bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole cells of recombinant CYP153A6-E. coli as biocatalyst for regioselective hydroxylation of monoterpenes
Authors: Cannazza Pietro , Rabuffetti Marco , Donzella Silvia , De Vitis Valerio , Contente Martina L. , de Oliveira Maria da Conceição Ferreira , de Mattos Marcos C. , Barbosa Francisco G. , de Souza Oliveira Ricardo Pinheiro , Pinto Andrea , Molinari Francesco , Romano Diego 
Published: April 2022

AbstractOptimized recombinant whole cells of E. coli bearing CYP153A6 were employed for catalyzing the hydroxylation of different monoterpene derivatives. In most cases, high selectivity was observed with exclusive hydroxylation of the allylic methyl group bound to the aliphatic ring. In the case of (R)- and (S)-carvone, hydroxylation occurred also on the other allylic methyl group, although to a lesser extent. Biotransformations carried out in fed-batch mode on (S)-limonene and α-terpineol showed that recombinant whole cells retained activity for at least 24 h, allowing for the recovery of 3.25 mg mL−1 of (S)-perillyl alcohol and 5.45 mg mL−1 of 7-hydroxy-α-terpineol, respectively.
Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma
Authors: Wang Xinmiao , Li Rui , Wu Luping , Chen Yang , Liu Shaopeng , Zhao Hui , Wang Yifan , Wang Lin , Shao Zhe 
Published: April 2022

Abstract
Background
Epigenetic reprogramming is involved in multiple steps of human cancer evolution and is mediated by a variety of chromatin-modifying enzymes. Specifically, the histone lysine methyltransferase KMT2D is among the most frequently mutated genes in oral squamous cell carcinoma (OSCC). However, the mechanisms by which KMT2D affects the development of OSCC remain unclear.

Results
In the present study, we found that the expression of KMT2D was elevated in OSCC compared to paracancerous specimens and was correlated with a more advanced tumor grade. More importantly, knockdown of KMT2D impaired their reconstitution in patient-derived organoids and decreased the expression of CD133 and β-catenin in OSCC cells. In in vitro and in vivo models, knockdown of KMT2D reduced the colony formation, migration and invasion abilities of OSCC cells and delayed tumor growth. Mechanistically, the dual-luciferase reporter and co-immunoprecipitation assays in two individual OSCC cell lines indicated that KMT2D may cooperate with MEF2A to promote the transcription activity of CTNNB1, thereby enhancing WNT signaling.

Conclusion
The upregulation of KMT2D contributes to stem-like properties in OSCC cells by sustaining the MEF2A-mediated transcriptional activity of CTNNB1.
Ultrasound screening protocol for osteochondrosis at selected predilection sites in thoroughbred yearlings
Authors: Hoey Seamus , O’Sullivan Jeremiah , Byrne Jennifer , Devine Sinead , Toomey William , McAllister Hester , Skelly Cliona 
Published: April 2022

AbstractBackgroundOsteochondrosis is a common condition of young horses where there is a failure of endochondral ossification, usually at predisposed sites. The estimated prevalence of osteochondrosis is 33–44%, with radiographic screening of yearlings being used to identify lesions. Radiography has two major limitations: poor sensitivity in detecting cartilaginous lesions and secondly, the exposure of the horse and personnel to ionising radiation. Ultrasonography allows imaging of the articular cartilage and subchondral bone margins and has been shown to be more sensitive in identifying osteochondrosis lesions. However, the ultrasonographic technique for examining joints is operator dependant, resulting in highly variable examinations, thus affecting its reliability and reproducibility as a screening test.ResultsA prospective observational clinical population study was undertaken involving twenty-two clinically normal weanling thoroughbred horses on-farm, describing a detailed protocol of the ultrasonographic examination technique for on-farm screening of common sites of osteochondral disease in the young horse, namely the carpal, metacarpophalangeal, stifle, tarsal and metatarsophalangeal joints.ConclusionTwo veterinary practitioners used the technique to illustrate the repeatability of the protocol. The step-by-step protocol provides a valuable, reliable, repeatable technique for veterinary professionals performing screening ultrasound in the field.
On the periodic boundary value problems for fractional nonautonomous differential equations with non-instantaneous impulses
Authors: Wang Xue , Zhu Bo 
Published: April 2022

AbstractIn this paper, we investigate periodic boundary value problems for Caputo type fractional semilinear nonautonomous differential equations with non-instantaneous impulses. By using semigroup theory combined with the measure of noncompactness and some fixed point theorems, the existence of PC-mild solutions for the equations is established. At the end, an example is presented to illustrate the application of our main results.
Numerical modeling of reinjection and tracer transport in a shallow aquifer, Nesjavellir Geothermal System, Iceland
Authors: Gómez-Díaz Esteban , Scott Samuel , Ratouis Thomas , Newson Juliet 
Published: April 2022

AbstractReinjection of excess water from the power production process in the Nesjavellir geothermal field has increased the temperature of shallow groundwaters, posing a risk to cold water wells used for the power plant as well as the ecosystem in Lake Thingvellir. Here, we present a numerical model of fluid flow and heat transport in the shallow reinjection zone to elucidate the flow path of reinjected liquid and the impact of reinjection on the temperature of groundwaters. The permeability structure of the model is based on a 3D geological model of the area. The numerical simulation is calibrated against underground water temperature data measured between 1998 and 2018 and data from a tracer test performed in 2018–2019. The model reproduces the overall temperature field and shows how a high-permeability lava flow together with rift-parallel normal faults act as permeable channels controlling fluid transport. If injection continues, the temperature along the lava flow increases considerably and spreads vertically to much deeper levels, generating a narrow warm zone along the main fault. If shallow injection ceases, temperature drops rapidly at the surface, but decreases slowly around the reinjection zone over 20 years. The numerical model in this study allowed a better characterization of the fracture–matrix interface and the porosity of post-glacial lava flows, contributing to sustainable management of the geothermal resource and the surrounding environment.
Relationship between morality, happiness, and social support among elderly people
Authors: Ahmed Hanem Ahmed Abd El Khalek , Mohamed Bothina Elsayed Said 
Published: April 2022

Abstract
Background
Various factors influence the lives of elders, such as social support, which is critical in preventing physical, psychological, and social difficulties. This study aimed to investigate the relationship between morality, happiness, and social support among elderly people. A descriptive correlational design was used in carrying out this study. This study was conducted at the Geriatric Social Club in El-Qawmia; Zagzig City, Alsharkia Governorate. A purposeful sample of 235 elders was recruited for this study. Four tools were used to collect data: A structured interview questionnaire, Philadelphia Geriatric Center Morale Scale (interview version), Multidimensional Scale of Perceived Social Support, and Oxford Happiness Questionnaire.

Results
Results denoted that less than half of studied elders had moderate score as regards morale scale and perceived social support. As well, less than three-fifths of them had moderate score regards total happiness. There were statistically significant relations between morality and both of age, income, chronic diseases, and taking medication. Perceived social support was positively statistically significantly related with elders’ gender, marital status, chronic diseases, medication, and income. There were statistical significant relations between elderly happiness level and working condition, income, and with whom they live. Elders’ perceived social support was highly positively statistically correlated with happiness and morality.

Conclusions
It was concluded that these variables were positively correlated with each one, where increasing perceived social support and feeling of happiness associated with enhancing feeling of morality among elders. The perceived social support and happiness were the key co-existences of elders’ morality. Therefore, it is recommended to implement caring and training programs focusing on enhancing social support in order to develop morale among elders. The country should provide various kinds of assistance to the elderly, such as counseling services.
Distinct Neoadjuvant Chemotherapy Response and 5-Year Outcome in Patients With Estrogen Receptor–Positive, Human Epidermal Growth Factor Receptor 2–Negative Breast Tumors That Reclassify as Basal-Type by the 80-Gene Signature
Authors: Whitworth Pat W. , Beitsch Peter D. , Pellicane James V. , Baron Paul L. , Lee Laura A. , Dul Carrie L. , Murray Mary K. , Gittleman Mark A. , Budway Raye J. , Rahman Rakhshanda Layeequr , Kelemen Pond R. , Dooley William C. , Rock David T. , Cowan Kenneth H. , Lesnikoski Beth-Ann , Barone Julie L. , Ashikari Andrew Y. , Dupree Beth B. , Wang Shiyu , Menicucci Andrea R. , Yoder Erin B. , Finn Christine , Corcoran Kate , Blumencranz Lisa E. , Audeh William ,   NBRST Investigators Group
Published: April 2022

PURPOSE The 80-gene molecular subtyping signature (80-GS) reclassifies a proportion of immunohistochemistry (IHC)-defined luminal breast cancers (estrogen receptor–positive [ER+], human epidermal growth factor receptor 2–negative [HER2–]) as Basal-Type. We report the association of 80-GS reclassification with neoadjuvant treatment response and 5-year outcome in patients with breast cancer. METHODS Neoadjuvant Breast Registry Symphony Trial (NBRST; NCT01479101 ) is an observational, prospective study that included 1,069 patients with early-stage breast cancer age 18-90 years who received neoadjuvant therapy. Pathologic complete response (pCR) and 5-year distant metastasis-free survival (DMFS) and overall survival (OS) were assessed in 477 patients with IHC-defined ER+, HER2– tumors and in a reference group of 229 patients with IHC-defined triple-negative breast cancer (TNBC). RESULTS 80-GS reclassified 15% of ER+, HER2– tumors (n = 73) as Basal-Type (ER+/Basal), which had similar pCR compared with TNBC/Basal tumors (34% v 38%; P = .52), and significantly higher pCR than ER+/Luminal A (2%; P < .001) and ER+/Luminal B (6%; P < .001) tumors. The 5-year DMFS (%, [95% CI]) was significantly lower for patients with ER+/Basal tumors (66% [52.6 to 77.3]), compared with those with ER+/Luminal A tumors (92.3% [85.2 to 96.1]) and ER+/Luminal B tumors (73.5% [44.5 to 79.3]). Importantly, patients with ER+/Basal or TNBC/Basal tumors that had a pCR exhibited significantly improved DMFS and OS compared with those with residual disease. By contrast, patients with ER+/Luminal B tumors had comparable 5-year DMFS and OS whether or not they achieved pCR. CONCLUSION Significant differences in chemosensitivity and 5-year outcome suggest patients with ER+/Basal molecular subtype may benefit from neoadjuvant regimens optimized for patients with TNBC/Basal tumors compared with patients with ER+/Luminal subtype. These data highlight the importance of identifying this subset of patients to improve treatment planning and long-term survival.
Maternal and Fetal Genetic Variation in Vitamin D Metabolism and Umbilical Cord Blood 25-Hydroxyvitamin D
Authors: Moon Rebecca J , Cooke Laura D F , D’Angelo Stefania , Curtis Elizabeth M , Titcombe Philip , Davies Justin H , Godfrey Keith M , Cleal Jane K , Lewis Rohan M , Cooper Cyrus , Harvey Nicholas C 
Published: April 2022

Abstract

Context
Single nucleotide polymorphisms (SNPs) in vitamin D metabolism pathway genes are associated with circulating 25-hydroxyvitamin D (25(OH)D) in adults. Less is known about the relationships between mother and offspring SNPs and umbilical cord blood 25(OH)D.

Objective
(1) To undertake a meta-analysis of the relationships of maternal and offspring SNPs in the vitamin D metabolism pathway and cord blood 25(OH)D in pregnant women including novel data; and (2) to examine these relationships in women who received antenatal cholecalciferol supplementation in a clinical trial.

Methods
Novel data analysis from an observational mother–offspring cohort study (Southampton Women’s Survey) and the MAVIDOS double-blind, randomized, placebo-controlled trial of 1000 IU/day cholecalciferol supplementation in pregnancy, and an electronic literature search of published studies in PubMed up to 31 July 2021. Studies reporting associations between rs12785878 (DHCR7), rs10741657 (CYP2R1), rs6013897 (CYP24A1), or rs2282679 (GC) and cord blood 25(OH)D. One published study was included in addition to the novel data analysis. Associations between both maternal and offspring SNPs at rs2282679 (GC) and rs12785878 (DHCR7), and cord blood 25(OH)D were identified. When maternal genotype was adjusted for offspring genotype, and vice versa, there was persisting evidence for associations with maternal rs12785878 (β [95% CI] 1.6 nmol/L [0.3, 2.8] per common allele), and offspring rs2282679 (β 3.1 nmol/L ]2.0, 4.4] per common allele). Maternal and offspring SNPs at rs1074657 and rs613897 were not associated with cord blood 25(OH)D.

Result
Associations between both maternal and offspring SNPs at rs2282679 (GC) and rs12785878 (DHCR7), and cord blood 25(OH)D were identified. When maternal genotype was adjusted for offspring genotype, and vice versa, there was persisting evidence for associations with maternal rs12785878 (β [95% CI] 1.6 nmol/L [0.3, 2.8] per common allele), and offspring rs2282679 (β 3.1 nmol/L ]2.0, 4.4] per common allele). Maternal and offspring SNPs at rs1074657 and rs613897 were not associated with cord blood 25(OH)D.

Conclusion
Common genetic variation in the vitamin D metabolism pathway is associated with umbilical cord blood 25(OH)D.
Approaching Addiction
Authors: Sussman Steve , Wright Erika 
Published: April 2022

AbstractThis essay seeks to illuminate the shifting definitions of addiction over time by providing a brief, anecdotal history of addiction as well as a review of how it has been conceptualized by the American Psychiatric Association’s DSM classification system. While all addictions have in common the attempt to experience an appetitive effect (satiety), preoccupation, loss of control, and negative or undesired consequences, including physical danger, social complaints, or inability to carry on one’s life roles, the meaning of addiction has not always been consistent. By approaching addiction from the historical/anecdotal and the official/medical perspectives, this essay provides a framework for working toward a common understanding of this complex phenomenon.
The History of the Subject and the Subject of History
Authors: Eng David L. 
Published: April 2022

AbstractThis article explores unexamined links between psychic and political theories of trauma to investigate the constitution of victims deserving and undeserving of reparation as they emerge in the context of the Holocaust, Hiroshima, and the Nuremberg and Tokyo War Tribunals. While genocide and nuclear catastrophe oriented the world imagination toward the specter of planetary annihilation, the “final solution” and the atomic bombings also cleave from one another in significant ways. In the space of postwar Europe, the history of the Holocaust is settled: Nazis were perpetrators and Jews were victims. In contrast, in the space of postwar Asia, there was and continues to be little historical consensus as to who were the victims and who were the perpetrators. As such, this article investigates how the uneven distribution of trauma across different geopolitical spaces and times carves out a privileged zone of exhausted and victimized humanity, with significant implications for addressing the injuries of violated human beings in Europe and elsewhere. Throughout, this article examines how psychoanalytic approaches to the history of the traumatized subject supplement the subject of Cold War history in search of an impossible historical consensus.
Crystal Structures and Conducting Properties of Mott Insulator (BEDT-BDS)PF6: Selenium Substitution Effect in the Parent (BEDT-BDT)PF6
Authors: Kadoya Tomofumi , Shishido Masaharu , Sugiura Shiori , Higashino Toshiki , Tahara Keishiro , Kubo Kazuya , Sasaki Takahiko , Yamada Jun-ichi 
Published: April 2022

Abstract
A new organic donor, viz. benzo[1,2-g:4,5-g′]bis(seleno[2,3-b][1,4]dithiin) (BEDT-BDS), which is a selenium analog of the parent benzo[1,2-g:4,5-g′]bis(thieno[2,3-b][1,4]dithiin), has been synthesized. The degree of “zigzag-N-shaped” molecular bending of BEDT-BDS, which is caused by the selenium substitution, is greater than that of BEDT-BDT. The N-shaped molecular bending in a radical-cation salt (BEDT-BDS)PF6 changes the S⋯S contact distances between the stacked molecules, and thus, a quasi-one-dimensional electronic structure is formed. According to the compositional formula and temperature dependence of resistivity, the PF6 salt is revealed to be a half-filled Mott insulator.
Performance and preference of problem-based learning (PBL) and lecture-based classes among medical students of Nepal
Authors: Adhikari Yadav Saroj , Poudel Sangeeta , Pandey Oshna , Jaiswal Dhiraj Prasad , Malla Bhupendra Prakash , Thakur Brajesh Kumar , Gautam Swotantra , Pandey Sirish Raj 
Published: April 2022

Background: PBL (problem based learning) is new active learning educational strategy that has been extensively tested and used in recent years. This study aims to compare students' understanding and knowledge retention when taught through PBL and lecture-based classes and compare PAHS students' perceptions of PBL and lectures in medical education. Methods: This is a cross-sectional study of medical students of a PBL based medical school in Nepal, a non-Western low-income country. Ethical approval was given by the institutional research committee of the Patan Academy of Health Sciences. Understanding and knowledge retention was assessed with 50 vignette-based multiple-choice questions, half of which were taught through PBL sessions, and the remaining half were taught in didactic lectures during basic science years of medical school. A separate pre-validated perception questionnaire was used to assess students' preferences regarding PBL and lectures. Results: Out of 107 students, 99 participated in the understanding and knowledge retention questionnaires and 107 completed perception questionnaires. Understanding and knowledge retention of students was found to be the same for topics taught by PBL and lectures, with median scores of 17 and s16, respectively. PBL were mostly preferred for the physiology (59.81%), pathology (51.40%) and pharmacology (53.27%) concepts, and lectures were mostly preferred for the anatomy (78.50%), biochemistry (45.79%), and microbiology (42.99%) topics. Students wanted the same concepts to be taught through both PBL and lectures, especially for anatomy. Conclusions: Understanding and knowledge retention is the same for topics taught by either PBL or lectures during the basic science years of undergraduate medical education. Students prefer PBL for physiology, pathology, and pharmacology-related concepts, conventional didactic lecture for physiology and microbiology, and a combination of lecture and PBL sessions for anatomy.
Evaluation of possible biological control of Fusarium concentricum sp. using plant extracts and antagonistic species of microbes in vitro
Authors: Hasan Mohammed Faruk , Islam Mohammed Asadul , Sikdar Biswanath 
Published: April 2022

Background: Fusarium concentricum is one of the most devastating fungi responsible for fruit and vegetable crops rot worldwide. The present study was designed to find an ecofriendly control measure for pathogenic Fusarium concentricum, using suitable bioagents. Methods: Medicinal plant extracts were evaluated or their antifungal activities against Fusarium concentricum using the poisoned food and serial dilution methods. Antagonistic potency of some nonpathogenic microbes was also assessed on Fusarium concentricum using the dual culture method. Results: Highest inhibition of growth of Fusarium concentricum was observed with 68.1% (0.389 mg per 90 mm Petri plate) of mycelia on Coccinia grandis plant leaf extract, in comparison to the control grown with 100.0% (1.22 mg/dish). The tested plants extract showed MIC values rages of 80-150 µg/ml on the isolated fungus. The highest inhibition of radial growth was observed using Trichoderma viride on Fusarium concentricum (46.01% inhibition). Conclusions: The findings of present study would be benevolent for antifungal drug development to control Fusarium concentricum causing fruit and vegetable rot.
Temporomandibular joints disorders (TMDs) prevalence and their relation to anxiety in dental students
Authors: Homeida Lujain , Felemban Emtenan , Kassar Wed , Ameen Mazen , Aldahlawi Salwa 
Published: April 2022

Background: Temporomandibular joint disorders (TMDs) are very common disorders affecting the population and causing pain. Researchers have reported a high prevalence of TMDs among university students due to increased distress. The aims of this study were to determine the frequency of TMDs in Umm al-Qura University (UQU) dental students using the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD), and to examine the relationship between anxiety, bruxism, and TMDs among those students. Methods: The DC/TMD pain screener questionnaire was completed by dental students. Students who reported TMD pain or had at least one positive TMD symptom were invited to the dental clinic for a full TMJ evaluation. History of bruxism was documented and an ultra-brief tool for detecting anxiety and depression (Patient Health Questionnaire-4 PHQ) was completed by all students. Results: A total of 240 students responded to the TMD pain screener in which 119 reported at least one TMJ symptom. Only 93 dental students presented to clinical examination in which 64.5% (n=60) of them had temporomandibular joint disorders. Disc displacement with reduction and local myalgia (38.7% & 32.25%, respectively) were the most frequent diagnosis. A total of 29% (n=27) of students had more than one diagnosis. Overall, 41 participants (44.09%) reported a positive response to the anxiety scale and (n=38) 40% of participants reported parafunctional habits. Both the history of bruxism and anxiety were significantly related to TMDs (P=0.0002) and also significantly higher in women of higher academic years (P≤0.01). Conclusions: This study found a high prevalence of TMDs among UQU dental students. Disc displacement with reduction was the most prevalent disorder. Bruxism and anxiety were associated with painful TMDs.