bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of vitamin D among male adolescents and young adults hospitalized with eating disorders
Authors: Nagata Jason M. , Grandis Anna , Bojorquez-Ramirez Paola , Nguyen Anthony , Downey Amanda E. , Ganson Kyle T. , Patel Khushi P. , Machen Vanessa I. , Buckelew Sara M. , Garber Andrea K. 
Published: July 2022

Abstract
Purpose
Medical complications of eating disorders in males are understudied compared to females, as is the case of vitamin D deficiency. The aim of this study was to assess vitamin D levels among male and female adolescents and young adults hospitalized for medical complications of eating disorders.

Methods
We retrospectively reviewed electronic medical records of patients aged 9–25 years (N = 565) admitted to the University of California, San Francisco Eating Disorders Program for medical instability, between May 2012 and August 2020. Serum vitamin D (25-hydroxy) level was assessed at admission as was history of prior calcium, vitamin D, or multivitamin supplementation. Linear regression was used to assess factors associated with vitamin D levels.

Results
A total of 93 males and 472 females met eligibility criteria (age 15.5 ± 2.8, 58.8% anorexia nervosa; admission body mass index 17.6 ± 2.91). Among male participants, 44.1% had 25-hydroxyvitamin D levels < 30 ng/mL, 18.3% had 25-hydroxyvitamin D levels < 20 ng/mL, and 8.6% had 25-hydroxyvitamin D levels < 12 ng/mL. There were no significant differences in 25-hydroxyvitamin D levels in males compared to females, except that a lower proportion (1.9%) of female participants had 25-hydroxyvitamin D levels < 12 ng/mL (p = 0.001). Only 3.2% of males reported calcium or vitamin D-specific supplementation prior to hospital admission, while 8.6% reported taking multivitamins. White race, prior calcium/vitamin D supplementation, and higher calcium levels were associated with higher vitamin D levels on admission.

Conclusions
Nearly half of patients admitted to the hospital for malnutrition secondary to eating disorders presented with low 25-hydroxyvitamin D levels; males were more likely than females to have severe vitamin D deficiency. These findings support vitamin D assessment as part of the routine medical/nutritional evaluation for hospitalized eating disorder patients, with particular attention on male populations.
Protective effects of coconut water against the intraperitoneal infused carbon tetrachloride-induced toxicity—evaluations of biochemical, haematological and histopathological profiles in rats
Authors: Emmanuel Okezie , Elekwa Ifeanyichukwu , Paul-Joseph Chidiebere , Ude Victor C. , Egedeuzu Ozioma G. , Ijioma Solomon N. , Amachaghi Victor Obioma , Uche-Ikonne Chikezie , Ugbogu Eziuche A. 
Published: July 2022

Abstract
Background
Coconut water is a relished beverage traditionally used as a remedy for childhood diarrhoea and gastroenteritis. It can be given to an individual who has hangover or suspected to have ingested a toxic substance. In this study, we evaluated the protective effect of coconut water against carbon tetrachloride (CCl4)-induced toxicity in rats.

Results
Administration of coconut water decreased significantly albumin, aspartate transaminase (AST), alanine transaminase (ALT), urea, creatinine, bicarbonate (HCO3−), total cholesterol, triglycerides, low-density lipoprotein, very low-density lipoprotein and malondialdehyde (MDA) levels compared to the non-pretreated group. Furthermore, high-density lipoprotein, glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) increased significantly (p < 0.05) in coconut water-pretreated groups compared to the negative control. There were no observed pathological changes in the coconut water-pretreated groups, but slight enlargement of the central veins and tubules was evident when compared to the negative control.

Conclusions
Therefore, intake of coconut water may be protective against toxicity induced by CCl4 as its pretreatment elevated antioxidant parameters (GSH, SOD and CAT) and did not alter biochemical parameters in rats.
The small GTPase BcSec4 is involved in conidiophore development, membrane integrity, and autophagy in Botrytis cinerea
Authors: Wang Guanbo , Zou Jian , Wang Yadi , Liang Wenxing , Li Delong 
Published: July 2022

AbstractSmall GTPases of the Rab family play important roles in membrane trafficking and autophagy. Previous studies have shown that the small GTPase Sec4 is involved in vegetative growth, protein secretion, and pathogenicity of fungal phytopathogens. In this study, the Rab GTPase BcSec4, an ortholog of the yeast Sec4p protein, was characterized in Botrytis cinerea. The ΔBcSEC4 mutant produces many abnormal conidiophores; it is thus defective in conidiation and displays attenuated pathogenicity. Notably, the germination and penetration of conidia are unaffected in the ΔBcSEC4 mutant. Further studies showed that the secretion of extracellular proteins was altered in the ΔBcSEC4 mutant, and the mutant showed impaired autophagy and defects in membrane integrity. Taken together, these results suggest that BcSec4 is critical for conidiophore development, membrane integrity, pathogenicity, and autophagy.
Properties of neighborhood for certain classes associated with complex order and m-q-p-valent functions with higher order
Authors: Madian Samar 
Published: July 2022

AbstractIn this paper, by using q-calculus (Jackson’s q-derivative) $$D_{q,p}$$

D

q
,
p

we defined new operator $$D_{\lambda ,q,p}^{n}f^{(m)}(z)$$

D

λ
,
q
,
p

n

f

(
m
)

(
z
)

. After that, we used this operator to introduce two new subclasses of multivalent analytic functions with complex order. Also, we obtained coefficients estimates and consequent inclusion relationships involving the $$N_{j,\delta ,m}^{p,q}(f)$$

N

j
,
δ
,
m

p
,
q

(
f
)

-neighborhood of these classes
Effects of prebiotics in combination with probiotics on intestinal hydrolase activity, microbial population and immunological biomarkers in SD rats fed an AIN-93G diet
Authors: Kim Min-Jeong , Jeon Dong-Gyeong , Lim Yong , Jang Insurk 
Published: July 2022

Abstract
Background
Gastrointestinal microbiota, which comprises hundreds of different types of microbes, biologically plays crucial roles in the host’s health. Probiotics (PRO) did not always have a positive benefit on the host, depending on strains of microbes and the physiochemical properties of prebiotics (PRE), indicating that the properties of PRE in combination with PRO might have different effects on the gut ecology. The aim of this study was to assess the effects of insoluble or soluble PRE with PRO on intestinal digestive hydrolase, the fecal microbes, and immunological biomarkers in SD rats fed an AIN-93G diet.

Results
Forty, 8-week-old SD rats were randomly assigned to 4 groups with 10 replicates in each; cellulose (CELL), cellulose + probiotics (CELPRO), oatmeal (OATS), and oatmeal + probiotics (OATPRO) groups. After 4-week feeding trial, rats were treated with saline or lipopolysaccharide (LPS, 1 mg/kg) to examine the alleviating effects of PRO and PRE on immunological responses. There was a significant (p < 0.05) decrease in feed intake of rats fed the oatmeal supplemented diet without affecting growth performance. Blood triglyceride was significantly (p < 0.05) decreased in rats fed the oatmeal diet, and aspartate aminotransferase (AST) was significantly (p < 0.05) decreased in rats fed the PRO supplemented diet. Intestinal maltase, sucrose, and lactase activities were significantly (p < 0.05) higher in rats fed PRO compared with rats not fed PRO. Rats fed the oatmeal showed a significant (p < 0.01) increase in the fecal colony forming units (CFU) of Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae compared with those fed cellulose. LPS-treated rats fed PRO showed a significant (p < 0.05) increase in blood secretory immunoglobulin A (sIgA) compared with those not fed PRO. The LPS-treated rats fed PRO resulted in decreased (p < 0.05) blood IL-6 compared with those not fed PRO, indicating that a dietary PRO alleviated inflammatory response in LPS-treated rats.

Conclusions
Dietary oatmeal increased fecal microbes, and PRO supplement resulted in increased intestinal hydrolase and immune functions of the host, demonstrating that soluble PRE with supplemented with PRO could be a more bioactive combination of synbiotics in SD rats.
Comparative prevalence of different types of viral hepatitis in the district Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan
Authors: Khan Muhammad Ashraf 
Published: July 2022

AbstractBackgroundViral hepatitis causes both liver inflammation and damage and is a serious health problem.MethodsThe indoor data of different types of hepatitis were collected from the official records of the administration of District Head Quarter Hospital Dera Ismail Khan for 2 years (2020–2021).ResultsA total of 1193 cases of viral hepatitis during the study period were found including viral hepatitis C accounted for 547 (45.9%) cases, followed by the co-infection of viral hepatitis A and E 367 (30.8%) cases, and viral hepatitis B 279 (23.4%) cases. March showed the highest prevalence of hepatitis (33.2%), followed by February (10.2%). The co-infection of hepatitis A and E showed a relatively higher prevalence in 6 months from May to November except September including a peak in June (76.5%) during the study period. While viral hepatitis B demonstrated a comparatively higher percentage prevalence in both February and September with a peak in February (68%), and viral hepatitis C is dominant in January, March, and December with a peak in March (83.8%).ConclusionAll types of viral hepatitis showed variation in prevalence over months and monthly variation concerning peak prevalence exists among different types of hepatitis. Both viral hepatitis A and E demonstrated similar trend in relative prevalence in both 2020 and 2021. Nevertheless, viral hepatitis A and B showed monthly and seasonal variation in relatively prevalence in both years. The study help in adopting strategies for the prevention of viral hepatitis in the study area.
Individual and national financial impacts of informal caring for people with mental illness in Australia, projected to 2030
Authors: Schofield Deborah , Zeppel Melanie J. B. , Tanton Robert , Veerman Jacob Lennert , Kelly Simon J. , Passey Megan E. , Shrestha Rupendra N. 
Published: July 2022

Background
Mental illness has a significant impact not only on patients, but also on their carers’ capacity to work.

Aims
To estimate the costs associated with lost labour force participation due to the provision of informal care for people with mental illness in Australia, such as income loss for carers and lost tax revenue and increased welfare payments for government, from 2015 to 2030.

Method
The output data of a microsimulation model Care&WorkMOD were analysed to project the financial costs of informal care for people with mental illness, from 2015 to 2030. Care&WorkMOD is a population-representative microsimulation model of the Australian population aged between 15 and 64 years, built using the Australian Bureau of Statistics Surveys of Disability, Ageing and Carers data and the data from other population-representative microsimulation models.

Results
The total annual national loss of income for all carers due to caring for someone with mental illness was projected to rise from AU$451 million (£219.6 million) in 2015 to AU$645 million (£314 million) in 2030 in real terms. For the government, the total annual lost tax revenue was projected to rise from AU$121 million (£58.9 million) in 2015 to AU$170 million (£82.8 million) in 2030 and welfare payments to increase from AU$170 million (£82.8 million) to AU$220 million (£107 million) in 2030.

Conclusions
The costs associated with lost labour force participation due to the provision of informal care for people with mental illness are projected to increase for both carers and government, with a widening income gap between informal carers and employed non-carers, putting carers at risk of increased inequality.
Advance Care Planning in Patients With Metastatic Cancer: A Quality Improvement Initiative
Authors: Oppenheim Steven , Figlin Robert A. , Seferian Edward G. , Reed Margaret , Irwin Scott A. , Rosen Bradley T. 
Published: July 2022

PURPOSE: An initiative aimed to increase the rate of advance care planning (ACP) activities for outpatients with metastatic cancer, an essential step to achieving goal concordant care. METHODS: Patients with metastatic cancer were identified by International Classification of Diseases-10 coding and later by oncologists' electronic health record documentation of metastatic tumor status. ACP activities were defined as either an ACP note, Advance Directive, Physician Orders for Life-Sustaining Therapy (POLST), or a Palliative Medicine (PM) consultation within the prior year. From 2017 to 2020, the initiative screened more than 5,000 total unique cancer patients per year. PM consultants were embedded in tumor boards, oncology care team meetings, and shared oncology clinic space. Quarterly reports were sent to 60 oncologists at three cancer care sites with data of their percentage of ACP activities for patients with metastatic cancer compared with their peers. Oncologists' identities were initially blinded, but later unblinded. Oncologists also received a monthly list of patients with metastatic cancer without ACP activities. RESULTS: The rate of ACP activities for patients with metastatic cancer increased from a baseline of 37% in July 2017 to 57% by the end of 2020. PM consultations increased from 12% to 39% and ACP notes increased from 16% to 29% during the same interval. There was no change in Advance Directive (17%-20%) or POLST completion (7%-6%). CONCLUSION: ACP activities are an essential step to achieve goal concordant care, and this initiative successfully increased ACP activities for patients with metastatic cancer. However, given that the main source of increased ACP activities during this initiative was PM referrals, further progress will depend upon strengthening the oncology care teams' ACP skills and motivation for completion.
The Missing Mortality Advantage for European Immigrants to the United States in the Early Twentieth Century
Authors: Bakhtiari Elyas 
Published: July 2022

Abstract
Immigrant populations typically have lower mortality rates and longer life expectancies than their nonimmigrant counterparts. This immigrant mortality advantage has been a recurrent finding in demographic and population health research focused on contemporary waves of immigration. However, historical data suggest that European immigrants to the United States in the early twentieth century had worse health and higher rates of mortality, yet it remains unclear why a mortality advantage was absent for immigrants during this period. This article combines Vital Statistics records and Lee–Carter mortality models to analyze mortality by nativity status for the U.S. White population from 1900 to 1960, examining variation by age, sex, time, and place. Contrary to contemporary expectations of a foreign-born mortality advantage, White immigrants had higher mortality rates in the early 1900s, with the largest foreign-born disadvantage among the youngest and oldest populations. Although foreign-born and U.S.-born White mortality rates trended toward convergence over time, the foreign-born mortality penalty remained into the 1950s. A decomposition analysis finds that immigrants' concentration in cities, which had higher rates of infectious disease mortality, accounted for nearly half of the nativity difference in 1900, and this place effect declined in subsequent decades. Additional evidence, such as a spike in mortality inequalities during the 1918 influenza pandemic, suggests that common explanations for the immigrant mortality advantage may be less influential in a context of high risk from infectious disease.
Respiratory syncytial virus disrupts the airway epithelial barrier by decreasing cortactin and destabilizing F-actin
Authors: Gao Nannan , Raduka Andjela , Rezaee Fariba 
Published: July 2022

ABSTRACTRespiratory syncytial virus (RSV) infection is the leading cause of acute lower respiratory tract infection in young children worldwide. Our group recently revealed that RSV infection disrupts the airway epithelial barrier in vitro and in vivo. However, the underlying molecular pathways were still elusive. Here, we report the critical roles of the filamentous actin (F-actin) network and actin-binding protein cortactin in RSV infection. We found that RSV infection causes F-actin depolymerization in 16HBE cells, and that stabilizing the F-actin network in infected cells reverses the epithelial barrier disruption. RSV infection also leads to significantly decreased cortactin in vitro and in vivo. Cortactin-knockout 16HBE cells presented barrier dysfunction, whereas overexpression of cortactin protected the epithelial barrier against RSV. The activity of Rap1 (which has Rap1A and Rap1B forms), one downstream target of cortactin, declined after RSV infection as well as in cortactin-knockout cells. Moreover, activating Rap1 attenuated RSV-induced epithelial barrier disruption. Our study proposes a key mechanism in which RSV disrupts the airway epithelial barrier via attenuating cortactin expression and destabilizing the F-actin network. The identified pathways will provide new targets for therapeutic intervention toward RSV-related disease.This article has an associated First Person interview with the first author of the paper.
Ontogeny can provide insight into the roles of natural and sexual selection in cricket cuticular hydrocarbon evolution
Authors: Simmons Leigh W. , Lovegrove Maxine , Du Xin (Bob) , Ren Yonglin , Thomas Melissa L. 
Published: July 2022

ABSTRACTThe often complex cocktails of hydrocarbon compounds found on the cuticles of insects can serve both naturally and sexually selected functions, contributing to an individual's ability to withstand water loss and attract mating partners. However, whether natural and sexual selection act synergistically or antagonistically on a species' cuticular hydrocarbon (CHC) profile remains unclear. Here, we examined the ontogeny of the CHC profile in a species of cricket, Teleogryllus oceanicus, while manipulating humidity during development. We predicted that juvenile crickets should produce only those compounds that contribute to desiccation resistance, while those compounds contributing specifically to male attractiveness should be produced only at sexual maturity. Further, if attractive CHCs come at a cost to desiccation resistance as predicted by some models of sexual selection, then males reared under low humidity should be constrained to invest less in attractive CHCs. Crickets reared under low humidity produced more long-chain methyl-branched alkanes, alkenes and alkadienes than did crickets reared under high humidity. The abundance of n-alkanes was unaffected by humidity treatment. Sexual dimorphism in the CHC profile was not apparent until adult emergence and became exaggerated 10 days after emergence, when crickets were sexually mature. Males produced more of the same compounds that were increased in both sexes under low humidity, but the humidity treatment did not interact with sex in determining CHC abundance. The data suggest that CHC profiles which protect crickets from desiccation might have synergistic effects on male attractiveness, as there was no evidence to suggest males trade-off a CHC profile produced in response to low humidity for one associated with sexual signalling.
Association between intestinal bacterial carriage, biomarkers of environmental enteric dysfunction, and stunting in rural Malawian children
Authors: Chaima David , Hart John , Pickering Harry , Burr Sarah , Maleta Kenneth , Kalua Khumbo , Bailey Robin , Holland Martin 
Published: July 2022

Background. Available data from murine studies suggest that intestinal bacteria may have a role in modulating growth phenotypes in the host. We investigated the prevalence of four gut bacteria known in murine models to impair growth (Bifidobacterium longum, Faecalibacterium prausnitzii, Dorea formicigenerans, and Akkermansia muciniphila), the level of fecal biomarkers of environmental enteric dysfunction (EED) and stunting in rural Malawian children. Methods. DNA and protein were extracted from fecal samples of rural Malawian children (aged 1-59 months) at a baseline cross-sectional survey in the Mangochi district of Malawi conducted within the framework of the Macrolides Oraux pour Réduire les Décès avec un Oeil sur la Résistance (MORDOR) trial. Intestinal carriage of bacteria was measured by PCR. Neopterin (NEO), myeloperoxidase (MPO), and alpha-1 antitrypsin (AAT), biomarkers of EED, were measured by an enzyme-linked immunosorbent assay (ELISA) test. Height-for-age Z (HAZ) score <-2 defined stunting. Tests of proportions and regression models were used to explore the relationship between bacterial carriage, EED, and stunting. Results. Fecal samples from 613 children were available for laboratory analyses. F. prausnitzii and D. formicigenerans were prevalent in over 70% of children while B. longum was the least prevalent. B. longum carriage in younger children was associated with elevated EED biomarkers. Two thirds of children had elevated NEO, 33% had elevated MPO, and 16% had elevated AAT. Stunting was found in 38%. No significant associations were found between EED biomarkers or intestinal bacteria carriage and stunting. Conclusion. Intestinal carriage of these four bacteria was not associated with stunting in Malawian children. Carriage was also not associated with EED, nor EED biomarker levels associated with stunting. Further factors acting in concert are necessary to impact EED, perturb growth, and alter gut bacterial carriage.
Identifying patterns of religiosity in adults from a large UK cohort using latent class analysis
Authors: Halstead Isaac , Heron Jon , Joinson Carol 
Published: July 2022

Background: Religiosity is a complex, multifaceted construct, comprising a variety of beliefs and behaviours. Much of the previous research that seeks to quantify religiosity has made use of variable-centred approaches, which place individuals on a continuum of religiosity. However, alternative approaches provide a way to examine different types of religiosity, represented by heterogeneous classes of religious (non)belief. The aim of this study was to apply a person-centred approach to understanding religious beliefs. Methods: The present study used latent class analysis to identify classes of belief and non-belief in mothers (n=12429) and their partners (n=9953) from the Avon Longitudinal Study of Parents and Children, a large cohort study based in the UK. For this, we used a range of religious belief indicators. We also examined a number of socioeconomic factors, to identify differences between classes, using logistic regression. Results: We identified four different classes of religiosity which we named Highly Religious, Moderately Religious, Agnostic, and Atheist, with similar configurations in both men and women. We also found that class membership was associated with several socioeconomic factors. Conclusions: The findings provide an insight into different patterns of religiosity in adults in the UK. These classes could be used as exposures in future studies of religiosity and how it relates to a variety of outcomes in both mothers, partners, and their offspring.