bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of 98 SNP markers in Japanese anchovy, Engraulis japonicus
Authors: Song Chenghui , Liu Yang , Wang Congcong 
Published: December 2022

Abstract
The Japanese anchovy, Engraulis japonicus, is an important economic fish that is distributed in the northwest Pacific Ocean. The effective assessment and management of the Engraulis japonicus fishery requires reliable information regarding its population’s genetic structure. The recent development of double digest restriction-site associated DNA sequencing (ddRAD-Seq) methods may contribute to the discovery of SNPs and the assessment of genetic structure. In our study, 98 single nucleotide polymorphism (SNP) markers were developed using ddRAD-Seq. The observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.3333 to 0.8000 and 0.2778–0.5000, respectively. The polymorphism information content (PIC) ranged from 0.239198 to 0.375. All loci have been substantiated to follow the Hardy–Weinberg equilibrium. These novel polymorphic SNP markers will play an important role in the genetic research of E.japonicus, which will be beneficial to the development and utilization of E.japonicus resources.
Significance of oxidative stress and antioxidant capacity (d-ROMs and BAP) tests as biomarkers of premature ovarian insufficiency: a case-control study
Authors: Kakinuma Kaoru , Kakinuma Toshiyuki 
Published: December 2022

Abstract
Background: Premature ovarian insufficiency (POI) is a condition that causes secondary amenorrhea due to ovarian hypofunction at an early stage (before the age of 40). Early follicular depletion results in not only increased morbidity of lifestyle diseases such as osteoporosis, myocardial infarction, and cerebral infarction due to low estrogen levels but also intractable infertility, thereby significantly reducing women’s quality of life. Given the continuum in weakened ovarian function, progressing from incipient ovarian failure (IOF) totransitional ovarian failure (TOF) and further to POI, it is necessary to develop biomarkers for predicting POI. There is a continuum of weakened ovarian function IOF, TOF, and transition to POI when it progresses.
In contrast, it has been suggested that mitochondrial function in eggs and cytotoxicity caused by accompanying reactive oxygen are factors that contribute to decreased remaining follicle count and egg quality owing to ovarian hypofunction.
By comprehensively evaluating the oxidative stress state in IOF and POI from both oxidative stress (diacron-reactive oxygen metabolites [d-ROMs]) test and antioxidant capacity (biological antioxidant potential [BAP]), this study aims to explore their possibilities as biomarkers for early detection of POI.
Methods: Among women under the age of 40 who visited our hospital from January 2021 to June 2022, we recruited 11 women in the POI group and 11 women in the IOF group, in addition to normal women of the same age in the control group, and measured their plasma d-ROMs and BAP.
Results: d-ROMs of the IOF and POI groups were both significantly higher than those of the control group (p < 0.001). No significant difference was found between the three groups in BAP. Oxidase stress indexes (d-ROMs/BAP x 100) of the IOF and POI groups were significantly higher than those of the control group (p < 0.001).
Conclusion: Oxidative stress (d-ROMs and oxidative stress index) levels of the IOF and POI groups were significantly higher than those of the control group, suggesting that the evaluation of the oxidative stress state is useful as an indicator for the early detection of POI.
“There’s absolutely no downside to this, I mean except community opposition:” A qualitative study of the acceptability of vending machines for harm reduction
Authors: Stewart Rebecca , Nicholas Cardamone , Loscalzo Emily , French Rachel , Lovelace Collin , Mowenn Winna Koe , Tahrini Ali , Lalley-Chareczko Linden , Brady Kathleen , Mandell David 
Published: December 2022

Abstract
Background:
Vending machines for harm reduction (VMHR) are an innovative approach to deliver life-saving materials, information, and treatment for hard-to-reach populations, particularly for persons who inject drugs. The current study explores stakeholders’ perspectives on the feasibility and acceptability of VMHR in Philadelphia.
Methods:
From October 2021 to February 2022, we conducted 31 semi-structured interviews with potential end-users, staff, and leadership at a local federally qualified health center, and community members. Trained coders extracted themes from interview transcripts across four key domains: materials and logistics, location, access, and community introduction.
Results:
All stakeholder groups endorsed using VMHR to provide supplies for wound care, fentanyl test strips, naloxone, and materials to connect individuals to treatment and other services. Providing syringes and medications for opioid use disorder elicited a broader range of acceptability. Stakeholders held varying views on the location and placement of the machines, but almost all agreed that a machine should be placed in the highest drug use areas. Stakeholders suggested several strategies to introduce and gain community acceptance of VMHR, including community education, one-on-one conversations with community members, and coupling the machine with safe disposal of syringes and information to link individuals to treatment.
Conclusions:
Stakeholders were generally receptive to VMHR. The current study findings are consistent with qualitative analyses from outside of the United States and contribute new ideas regarding the anticipated community response and best methods for introducing these machines to a community. With thoughtful planning and design, VMHR could be a feasible and acceptable modality to reduce death and disease transmission associated with the opioid and HIV epidemics in cities like Philadelphia.
Advancements in Hepatocellular Carcinoma: Potential Preclinical Drugs and their Future
Authors: Baytas Sultan Nacak , Butt Noor-ul-Huda 
Published: December 2022

Abstract:
Hepatocellular carcinoma (HCC) is one of the foremost causes of tumor-affiliated demises globally.
The HCC treatment has undergone numerous developments in terms of both drug and non-drug treatments. The
United States Food and Drug Administration (FDA) has authorized the usage of a variety of drugs for the treatment
of HCC in recent years, involving multi-kinase inhibitors (lenvatinib, regorafenib, ramucirumab, and
cabozantinib), immune checkpoint inhibitors (ICIs) (pembrolizumab and nivolumab), and combination therapies
like atezolizumab along with bevacizumab. There are currently over a thousand ongoing clinical and preclinical
studies for novel HCC drugs, which portrays a competent setting in the field. This review discusses the
i. FDA-approved HCC drugs, their molecular targets, safety profiles, and potential disadvantages; ii. The intrial
agents/drugs, their molecular targets, and possible benefits compared to alternatives, and iii. The current
and future status of potential preclinical drugs with novel therapeutic targets for HCC. Consequently, existing
drug treatments and novel strategies with their balanced consumption could ensure a promising future for a universal
remedy of HCC in the near future.
Personalized Help-Seeking Web Application for Chinese-Speaking International University Students: Development and Usability Study
Authors: Choi Isabella , Mestroni Gemma , Hunt Caroline , Glozier Nick 
Published: December 2022

Background
The mental health of international students is a growing concern for education providers, students, and their families. Chinese international students have low rates of help seeking owing to language, stigma, and mental health literacy barriers. Web-based help-seeking interventions may improve the rate of help seeking among Chinese international students.

Objective
This study aimed to describe the development of a mental well-being web app providing personalized feedback and tailored psychoeducation and resources to support help seeking among international university students whose first language is Chinese and test the web application’s uptake and engagement.

Methods
The bilingual MindYourHead web application contains 6 in-app assessments for various areas of mental health, and users are provided with personalized feedback on symptom severity, psychoeducation tailored to the person’s symptoms and information about relevant interventions, and tailored links to external resources and mental health services. A feasibility study was conducted within a school at the University of Sydney to examine the uptake and engagement of the web application among Chinese international students and any demographic characteristics or help-seeking attitudes or intentions that were associated with its engagement.

Results
A total of 130 Chinese international students signed up on the web application. There was an uptake of 13.4% (122/908) in the schools’ Chinese student enrollment. Most participants (76/130, 58.5%) preferred to use the web application in Chinese and used informal but not formal help for their mental health. There was considerable attrition owing to a design issue, and only 46 students gained access to the full content of the web application. Of these, 67% (31/46) of participants completed 1 or more of the in-app mental well-being assessments. The most commonly engaged in-app assessments were distress (23/31, 74%), stress (17/31, 55%), and sleep (15/31, 48%), with the majority scoring within the moderate- or high-risk level of the score range. In total, 10% (9/81) of the completed in-app assessments led to clicks to external resources or services. No demographic or help-seeking intentions or attitudes were associated with web-application engagement.

Conclusions
There were promising levels of demand, uptake, and engagement with the MindYourHead web application. The web application appears to attract students who wished to access mental health information in their native language, those who had poor mental health in the past but relied on informal support, and those who were at moderate or high risk of poor mental well-being. Further research is required to explore ways to improve uptake and engagement and to test the efficacy of the web application on Chinese international students’ mental health literacy, stigma, and help seeking.
The Current Knowledge, Attitudes, and Practices of the Neglected Methodology of Web-Based Questionnaires Among Chinese Health Workers: Web-Based Questionnaire Study
Authors: Fang Heping , Lv Yuxin , Chen Lin , Zhang Xuan , Hu Yan 
Published: December 2022

Background
Web-based questionnaire (WBQ) surveys are popular, but the quality of reporting WBQ survey research is uneven and unsatisfactory worldwide. Education and training on WBQ methodology may be necessary. However, the current knowledge, attitudes, and practices (KAP) of its methodology remain unknown.

Objective
We investigated the KAP of WBQ methodology among Chinese health workers for the first time to clarify the possible reasons for the unsatisfactory reporting quality of WBQ survey research from China’s experience, aiming to provide a basis for improvement.

Methods
We developed a structured WBQ based on the current recommendations and knowledge and investigated 458 health workers from June 7 to July 6, 2022. A total of 381 valid questionnaires were analyzed after data processing. We defined 50% and 75% as “qualified” and “satisfactory” in knowledge and practice topics to describe the results and analyzed the basic characteristics of the participants who had difficulties in conducting WBQ survey research.

Results
A total of 215 (56.4%) participants had used WBQs for investigation, mostly more than 2 times (88.3%), but only 95 (44.2%) of them had ever received methodological training. A total of 134 (62.3%) users believed that WBQs were practical, but 126 (58.6%) had doubts about the reliability of the results. Most of the knowledge and practice topics did not reach a satisfactory level, and some even did not reach a qualified level. A total of 95 (44.2%)-136 (63.3%) of the users had reported difficulties in conducting WBQ survey research, and different participants could have different difficulties according to their characteristics. In addition, 191 (88.8%) users believed training was necessary.

Conclusions
We found that Chinese health workers seriously underestimated and neglected the importance of the WBQ methodology, which may be an important reason for the reduced reporting quality of WBQ survey research. Medical educators need to strengthen methodological training on WBQs, which may help to improve the quality of WBQ survey research.
Effect of Peat Water Mix with Borehole Water on Mortality of African Catfish
Authors: Ardianor Ardianor , Handayani Tutwuri , Yusuf Noor Syarifuddin , Elvince Rosana 
Published: December 2022

Generally, cultured fish are suitable in media with a pH of around 7, like mountainous river and borehole water. However, peat water is known with low pH. It is not suitable for fish culture, especially for fingerlings. This study was conducted to examine the survival of African catfish fingerling (Clarias gariepinus) in the mixture of peat and borehole water with different pHs. Survival test of African catfish as subject applied bioassay method, LC50-96 hours. Peat water as the agent was arranged for 11 concentration levels of 0% - 100% with no replication and placed in plastic basins with 20 liters each. Catfish fingerlings were used for subject fish (size 10-15 cm) and 10 individuals in each basin. Water quality, mortality, and behavior of fish were observed. Results showed that the survival ability of African catfish on peat water, the LC50-96 hours was 63.8%, with 95% CI between 53.3% and 76.4% in concentration. Behavioral response of fish fingerling exposed to peat water ≥ 40% in the beginning 2 hours was frequently swimming up to surface. Lethal responses fingerlings were frequently taking up oxygen to the water surface, horizontally swimming, and moving vertically before death. After death, the fish ran out of mucus, and the skin looked peeling and bruising.
Influence of In-stream Ecosystem Restoration Techniques on the Fish Ecology of the River Nabongo in Eastern Uganda
Authors: Turyahabwe Remigio , Nabalegwa Muhamud Wambedde , Asaba Joyfred , Mulabbi Andrew 
Published: December 2022

The study was the first in Uganda to assess the responses of fish community assemblages to introduced woody debris structures in a tropical river in Eastern Uganda. For comparison purposes, two different woody debris structures (simple and complex) were introduced in river Nabongo, and their effect on fish assemblage and feeding was established based on experiments conducted in two heterogeneous stream environments (a pool and a riffle). Results showed that sampling plots treated with restoration structures registered higher fish species richness, diversity, and abundance than sampling plots without restoration structures (control plots) at each site. The study (experiment) applied a stratified sampling design which used purposive identification of a pool and a riffle in River Nabongo Catchment. Fish were captured using a drift net, an electro-fishing gear, and a hand net. Data were analysed using a one-way ANOVA generated from STATA version 14. At the pool site, total fish density varied significantly from plot to plot (P<0.05) but was highest in the complex structures with 64±1.08 fishes/m2 and lowest with 24±0.82 fishes/m2 in untreated plots. K-factor did not vary significantly in untreated plots at the pool site but significantly differed from treated plots at P<0.05. The relative abundance of fish species at the pool site was highest in the complex structures with 40.7±0.66% and 21.5±0.42% before structures but was least in the control plot, varying significantly from plot to plot at P<0.05. It was concluded that woody debris restoration is an effective stream restoration technique. Fish individuals, trophic groups, and taxa more densely colonised sampling plots that had structures than those that did not have structures.
HUBUNGAN ORIENTASI PERILAKU PEMIMPIN TERHADAP EMPLOYEE ENGAGEMENT DI KANTOR SUCOFINDO CABANG JAMBI DENGAN ‘TRUST’ SEBAGAI VARIABEL MEDIATOR
Authors: Huda Nurul , Rosita Sry 
Published: December 2022

Penelitian ini bertujuan untuk mengidentifikasi hubungan antara orientasi perilaku pemimpin dan employee engagement pegawati PT.Sucofindo (Persero) Cabang Jambi melalui trust. Penelitian ini menggunakan metode penelitian yang bersifat deskriptif kuantitatif dengan menggunakan kuisioner sebagai teknik pengumpulan data dan software partial least square (PLS) sebagai alat analisis data. Berdasarkan hasil analisis yang dilakukan, terdapat hubungan yang positif dan signifikan antara orientasi perilaku pemimpin dengan employee engagement, orientasi perilaku pemimpin dengan trust, trust dengan employee engagement, dan hubungan yang positif dan signifikan juga ditemukan pada hubungan orientasi perilaku pemimpin dan employee engagement melalui trust. Hasil penelitian ini menunjukkan bahwa PT. Sucofindo (Persero) Cabang Jambi harus dapat selalu meningkatkan gaya kepimimpinan yang memiliki orientasi dalam berperilaku serta dapat meningkatkan trust pegawai terhadap pemimpin itu sendiri maupun organisasi secara umum.
Kata Kunci: Orientasi perilaku pemimpin, employee engagement, trust, PT. Sucofindo (Persero) Cabang Jambi
PENGARUH BUDAYA ORGANISASI TERHADAP KOMITMEN PEGAWAI MELALUI KEPUASAN KERJA PEGAWAI DI PT.SUCOFINDO (PERSERO) CABANG JAMBI
Authors: Nasution Akhmad Sulaiman , Edward Edward 
Published: December 2022

Penelitian ini bertujuan untuk mengidentifikasi hubungan antara budaya organisasi dan komitmen pegawai melalui kepuasan kerja pada pegawai PT. Sucofindo (Persero) Cabang Jambi. Jenis penelitian yang digunakan adalah penelitian kuantitatif. Data yang dianalisa diperoleh melalui kuesioner yang diberikan kepada 57 responden, kemudian akan diolah menggunakan software partial least square (PLS). Terdapat empat tahapan analisa ynag dilakukan, yaitu outer model, inner model dan pengujian hipotesis. Berdasarkan analisa yang dilakukan ditemukan bahwa terdapat hubungan yang positif dan signifikan antara budaya organisasi dan kepuasan kerja dengan nilai signifikan 0,003, budaya organisasi dan kepuasan kerja dengan komitmen pegawai nilai signifikan 0,003,kepuasan kerja dan komitmen dengan nilai signifikan 0,000 serta hubungan yang positif dan signifikan juga ditemukan pada hubungan budaya organisasi dan komitmen melalui kepuasan kerja dengan nilai signifikan 0,026. Hasil penelitian ini menunjukkan bahwa PT. Sucofindo (Persero) Cabang Jambi harus terus meningkatkan budaya kerja sehingga akan tercipta kepuasan kerja dan dengan sendirinya komitmen pegawai dapat terbentuk.
Kata Kunci : Budaya Organisasi, Kepuasan Kerja, komitmen pegawai
PENGARUH PROMOSI VISUAL, FASILITAS DAN DAYA TARIK WISATA TERHADAP MINAT BERKUNJUNG WISATAWAN DI CANDI MUARO JAMBI
Authors: Dahmiri Dahmiri , Bhayangkari Sylvia Kartika Wulan 
Published: December 2022

Objek wisata Candi Muaro Jambi menjadi salah satu keunggulan wisata di Kabupaten Muaro Jambi. Pemerintah daerah Kabupaten Muaro Jambi terus melakukan pengembangan terhadap kawasan Candi Muaro Jambi. Berbagai upaya yang dilakukan dalam pengembangan dari sisi promosi, peningkatan fasilitas dan daya tarik agar mampu meningkatkan minat wisatawan berkunjung. Tujuan dari penelitian ini adalah untuk menganalisis pengaruh promosi visual, fasilitas dan daya tarik wisata terhadap minat berkunjung wisatawan di Candi Muaro Jambi. Rancangan penelitian yang digunakan dalam penelitian ini adalah deskriptif dan statistik. Jumlah sampel dalam penelitian ini berjumlah 150 orang. Metode analisis statistik menggunakan regresi linier berganda, analisis koefisien determinasi, pengujian signifikansi simultan (uji F) dan pengujian signifikansi parsial (uji t). Hasil penelitian ini menunjukkan bahwa secara parsial variabel promosi visual, fasilitas dan daya tarik wisata memiliki pengaruh yang signifikan terhadap minat beberkunjung di Candi Muaro Jambi. Secara simultan variabel promosi visual, fasilitas dan daya tarik wisata memiliki pengaruh yang signifikan terhadap minat berkunjung di Candi Muaro Jambi.
Analisis Efisiensi Pemasaran Bawang Daun (Allium fistulosum. L) di Kecamatan Bandungan Kabupaten Semarang
Authors: Saragih Manna , Budiraharjo Kustopo , Ekowati Titik 
Published: December 2022

Penjualan bawang daun di Kecamatan Bandungan memiliki marjin besar antara produsen dan konsumen. Penelitian ini bertujuan untuk mengidentifikasi saluran pemasaran bawang daun, menganalisis efisiensi pemasaran, serta menganalisis pengaruh harga jual produsen, harga beli konsumen, dan biaya pemasaran terhadap efisiensi pemasaran. Penelitian ini dilaksanakan pada bulan Januari hingga Februari 2022. Lokasi penelitian dilaksanakan di dua desa, yaitu Desa Mlilir dan Sidomukti. Lokasi penelitian ditentukan secara purposive. Metode penelitian menggunakan metode survei. Pengambilan sampel di tingkat petani menggunakan quota sampling berjumlah 40 orang dan pengambilan sampel lembaga pemasaran menggunakan snowball sampling berjumlah 26 orang. Metode analisis data menggunakan analisis deskriptif yaitu pola saluran pemasaran dan fungsi-fungsi pemasaran bawang daun, dan analisis kuantitatif yaitu perhitungan marjin pemasaran, farmer’s share, efisiensi pemasaran, uji beda one sample T-test, dan regresi linear berganda. Hasil penelitian menunjukkan terdapat empat saluran pemasaran bawang daun di Kecamatan Bandungan yaitu saluran 1 (petani - pedagang pengecer - konsumen), saluran 2 (petani - pedagang besar - pedagang pengecer - konsumen), saluran 3 (petani - pedagang pengepul - pedagang pengecer - konsumen), dan saluran 4 (petani - pedagang pengepul - pedagang besar - pedagang pengecer - konsumen). Berdasarkan marjin pemasaran dan farmer’s share, saluran yang paling efisien diperoleh pada saluran 1, masing-masing nilai marjin pemasaran dan farmer’s share sebesar Rp2.000 dan 83,33%. Diperoleh perbedaan yang signifikan antara efisiensi pemasaran dengan signifikan sebesar 0,000<0,05 dengan standar efisiensi pemasaran. Faktor harga di tingkat petani, harga di tingkat konsumen, dan biaya pemasaran berpengaruh secara serempak dan parsial terhadap efisiensi pemasaran dengan signifikan sebesar 0,000<0,05.
Pengaruh Sosial Ekonomi Penyuluh Terhadap Kinerja Penyuluh Pertanian di Kabupaten Donggala
Authors: Laepo Nursyanti Dj , Antara Made , Muis Abdul 
Published: December 2022

Peran penting penyuluh pertanian dalam pembangunan pertanian adalah sebagai agen perubahan, penyuluh merupakan ujung tombak yang langsung berhubungan dengan petani, Tujuan penelitian ini untuk mengetahui hubungan pengaruh sosial ekonomi penyuluh terhadap kinerja penyuluh pertanian di Kabupaten Donggala. Jumlah sampel dalam penelitian ini sebanyak 49 orang petugas penyuluh pertanian yang berstatus PNS. Penentuan sampel dilakukan dengan menggunakan teknik proportionate stratified random sampling (pengambilan sampel acak distrafikasi berimbang). Alat analisis data yang digunakan dalam penelitian ini menggunakan program SPSS versi 22.0. Hasil analisis deskriptif penilaian kinerja penyuluh pertanian yang bersatus PNS di Kabupaten Donggala berada pada kategori baik. Rataan skor kinerja penyuluh responden adalah 49,00% dan hasil analisis regresi linier berganda menggunakan fungsi Cobb-Douglas diperoleh bahwa faktor sosial ekonomi penyuluh yang terdiri atas umur, lama studi, masa kerja, jumlah tanggungan keluarga, jumlah pendapatan serta jarak tempat tinggal dengan wilayah kerja secara simultan berpengaruh signifikan terhadap kinerja petugas penyuluh pertanian di Kabupaten Donggala.