bluuebunny/crossref_metadata_2025_split huggingface dataset:
Divergent Hydrological Responses to Forest Expansion in Dry and Wet Basins of China: Implications for Future Afforestation Planning
Authors: Xue Baolin , A Yinglan , Wang Guoqiang , Helman David , Sun Ge , Tao Shengli , Liu Tingxi , Yan Denghua , Zhao Tongtiegang , Zhang Hongbo , Chen Lihua , Sun Wenchao , Xiao Jingfeng 
Published: May 2022

AbstractAfforestation to control soil erosion has been implemented throughout China over the past few decades. The long‐term hydrological effects, such as total water yield and baseflow, of this large‐scale anthropogenic activity remain unclear. Using six decades of hydrologic observations and remote sensing data, we explore the hydrological responses to forest expansion in four basins with contrasting climates across China. No significant change in runoff was found for the period 1970–2012 for the cold and dry Hailar River Basin in northeastern China. However, both forest expansion and reduced precipitation contributed to the runoff reduction after afforestation since the late 1990s. Similarly, afforestation and drying climate since the mid‐1990s induced a significant decrease in runoff for the Weihe River Basin in semi‐arid northwestern China. In contrast, the two wet basins in the humid southern China, Ganjiang River Basin and Dongjiang River Basin, showed insignificant changes in total runoff during their study periods. However, the baseflow in the winter dry seasons in these two watersheds significantly increased since the 1950s. Our results highlight the long‐term variable effects of forest expansion and local climatic variability on basin hydrology in different climatic regions. This study suggests that landuse change in the humid study watersheds did not cause dramatic change in river flow and that region‐specific afforestation policy should be considered to deal with forestation‐water quantity trade‐off. Conclusions from this study can help improve decision‐making for ecological restoration policies and water resource management in China and other countries where intensive afforestation efforts are taking place.
Climatic Influences on Summer Use of Winter Precipitation by Trees
Authors: Goldsmith Gregory R. , Allen Scott T. , Braun Sabine , Siegwolf Rolf T. W. , Kirchner James W. 
Published: May 2022

AbstractTrees in seasonal climates may use water originating from both winter and summer precipitation. However, the seasonal origins of water used by trees have not been systematically studied. We used stable isotopes of water to compare the seasonal origins of water found in three common tree species across 24 Swiss forest sites sampled in two different years. Water from winter precipitation was observed in trees at most sites, even at the peak of summer, although the relative representation of seasonal sources differed by species. However, the representation of winter precipitation in trees decreased with site mean annual precipitation in both years; additionally, it was generally lower in the cooler and wetter year. Together, these relationships show that precipitation amount influenced the seasonal origin of water taken up by trees across both time and space. These results suggest higher turnover of the plant‐available soil‐water pool in wetter sites and wetter years.
Sand Transport on Titan: A Sticky Problem
Authors: Lorenz Ralph D. 
Published: May 2022

AbstractSaltation modeling by Comola et al. (2022, https://doi.org/10.1029/2022GL097913) considering a higher interparticle cohesion suggests smaller sand size on Titan than previously assumed, with possibly frequent sand transport. These predictions are testable by the Dragonfly mission presently under development.
Loss of KDM4B impairs osteogenic differentiation of OMSCs and promotes oral bone aging
Authors: Deng Peng , Chang Insoon , Wang Jiongke , Badreldin Amr A. , Li Xiyao , Yu Bo , Wang Cun-Yu 
Published: May 2022

AbstractAging of craniofacial skeleton significantly impairs the repair and regeneration of trauma-induced bony defects, and complicates dental treatment outcomes. Age-related alveolar bone loss could be attributed to decreased progenitor pool through senescence, imbalance in bone metabolism and bone-fat ratio. Mesenchymal stem cells isolated from oral bones (OMSCs) have distinct lineage propensities and characteristics compared to MSCs from long bones, and are more suited for craniofacial regeneration. However, the effect of epigenetic modifications regulating OMSC differentiation and senescence in aging has not yet been investigated. In this study, we found that the histone demethylase KDM4B plays an essential role in regulating the osteogenesis of OMSCs and oral bone aging. Loss of KDM4B in OMSCs leads to inhibition of osteogenesis. Moreover, KDM4B loss promoted adipogenesis and OMSC senescence which further impairs bone-fat balance in the mandible. Together, our data suggest that KDM4B may underpin the molecular mechanisms of OMSC fate determination and alveolar bone homeostasis in skeletal aging, and present as a promising therapeutic target for addressing craniofacial skeletal defects associated with age-related deteriorations.
Physics and applications of Raman distributed optical fiber sensing
Authors: Li Jian , Zhang Mingjiang 
Published: May 2022

AbstractRaman distributed optical fiber sensing has been demonstrated to be a mature and versatile scheme that presents great flexibility and effectivity for the distributed temperature measurement of a wide range of engineering applications over other established techniques. The past decades have witnessed its rapid development and extensive applicability ranging from scientific researches to industrial manufacturing. However, there are four theoretical or technical bottlenecks in traditional Raman distributed optical fiber sensing: (i) The difference in the Raman optical attenuation, a low signal-to-noise ratio (SNR) of the system and the fixed error of the Raman demodulation equation restrict the temperature measurement accuracy of the system. {ii) The sensing distance and spatial resolution cannot be reconciled. (iii) There is a contradiction between the SNR and measurement time of the system. (iv) Raman distributed optical fiber sensing cannot perform dual-parameter detection. Based on the above theoretical and technical bottlenecks, advances in performance enhancements and typical applications of Raman distributed optical fiber sensing are reviewed in this paper. Integration of this optical system technology with knowledge based, that is, demodulation technology etc. can further the performance and accuracy of these systems.
A biosensing system using a multiparameter nonlinear dynamic analysis of cardiomyocyte beating for drug-induced arrhythmia recognition
Authors: Wang Hao , Wu Yue , Zou Quchao , Yang Wenjian , Xu Zhongyuan , Dong Hao , Zhu Zhijing , Wang Depeng , Wang Tianxing , Hu Ning , Zhang Diming 
Published: May 2022

AbstractCardiovascular disease is the number one cause of death in humans. Therefore, cardiotoxicity is one of the most important adverse effects assessed by arrhythmia recognition in drug development. Recently, cell-based techniques developed for arrhythmia recognition primarily employ linear methods such as time-domain analysis that detect and compare individual waveforms and thus fall short in some applications that require automated and efficient arrhythmia recognition from large datasets. We carried out the first report to develop a biosensing system that integrated impedance measurement and multiparameter nonlinear dynamic algorithm (MNDA) analysis for drug-induced arrhythmia recognition and classification. The biosensing system cultured cardiomyocytes as physiologically relevant models, used interdigitated electrodes to detect the mechanical beating of the cardiomyocytes, and employed MNDA analysis to recognize drug-induced arrhythmia from the cardiomyocyte beating recording. The best performing MNDA parameter, approximate entropy, enabled the system to recognize the appearance of sertindole- and norepinephrine-induced arrhythmia in the recording. The MNDA reconstruction in phase space enabled the system to classify the different arrhythmias and quantify the severity of arrhythmia. This new biosensing system utilizing MNDA provides a promising and alternative method for drug-induced arrhythmia recognition and classification in cardiological and pharmaceutical applications.
Longitudinal trajectory of response to electroconvulsive therapy associated with transient immune response & white matter alteration post-stimulation
Authors: Andreou Blake , Reid Benjamin , Lyall Amanda E. , Cetin-Karayumak Suheyla , Kubicki Antoni , Espinoza Randall , Kruse Jennifer , Narr Katherine L. , Kubicki Marek 
Published: May 2022

AbstractResearch suggests electroconvulsive therapy (ECT) induces an acute neuroinflammatory response and changes in white matter (WM) structural connectivity. However, whether these processes are related, either to each other or to eventual treatment outcomes, has yet to be determined. We examined the relationship between levels of peripheral pro-inflammatory cytokines and diffusion imaging-indexed changes in WM microstructure in individuals with treatment-resistant depression (TRD) who underwent ECT. Forty-two patients were assessed at baseline, after their second ECT (T2), and after completion of ECT (T3). A Montgomery Åsberg Depression Rating Scale improvement of >50% post-ECT defined ECT-responders (n = 19) from non-responders (n = 23). Thirty-four controls were also examined. Tissue-specific fractional anisotropy (FAt) was estimated using diffusion imaging data and the Free-Water method in 17 WM tracts. Inflammatory panels were evaluated from peripheral blood. Cytokines were examined to characterize the association between potential ECT-induced changes in an inflammatory state and WM microstructure. Longitudinal trajectories of both measures were also examined separately for ECT-responders and non-responders. Patients exhibited elevated Interleukin-8 (IL-8) levels at baseline compared to controls. In patients, correlations between IL-8 and FAt changes from baseline to T2 were significant in the positive direction in the right superior longitudinal fasciculus (R-SLF) and right cingulum (R-CB) (psig = 0.003). In these tracts, linear mixed-effects models revealed that trajectories of IL-8 and FAt were significantly positively correlated across all time points in responders, but not non-responders (R-CB-p = .001; R-SLF-p = 0.008). Our results suggest that response to ECT in TRD may be mediated by IL-8 and WM microstructure.
Circular RNA circVAMP3 promotes aerobic glycolysis and proliferation by regulating LDHA in renal cell carcinoma
Authors: Li Jun , Zhang Qian , Guan Yupeng , Liao Dingzhun , Jiang Donggen , Xiong Haiyun , Zhan Hengji , Pang Jun 
Published: May 2022

AbstractMetabolic dysfunction is seen in cancer cells where increased glycolysis provides energy for growth. Circular RNAs (circRNAs) are thought to assist in glucose metabolism and the switch to glycolysis. Through screening, we found that circVAMP3 was necessary for both glycolytic and proliferative activities in renal cell carcinoma (RCC). Furthermore, circVAMP3 expression was elevated in RCC patients in correspondence with TNM stage. Mechanistically, circVAMP3 was observed to interact directly with lactate dehydrogenase A (LDHA) and modulate its activity. The circVAMP3–LDHA interaction facilitated LDHA phosphorylation at tyrosine 10 (Y10) catalyzed by the upstream kinase fibroblast growth factor receptor type 1 (FGFR1). Therefore, this study reveals a novel molecular mechanism by which circVAMP3 promotes glycolysis and proliferation through regulating the enzymatic activity of glycolytic enzyme, suggesting that circVAMP3 may represent an RCC biomarker and treatment target.
DNA hypermethylation modification promotes the development of hepatocellular carcinoma by depressing the tumor suppressor gene ZNF334
Authors: Sun Dapeng , Gan Xiaojie , Liu Lei , Yang Yuan , Ding Dongyang , Li Wen , Jiang Junyao , Ding Wenbin , Zhao Linghao , Hou Guojun , Yu Jian , Wang Jie , Yang Fu , Yuan Shengxian , Zhou Weiping 
Published: May 2022

AbstractDNA methylation plays a pivotal role in the development and progression of tumors. However, studies focused on the dynamic changes of DNA methylation in the development of hepatocellular carcinoma (HCC) are rare. To systematically illustrate the dynamic DNA methylation alternation from premalignant to early-stage liver cancer with the same genetic background, this study enrolled 5 HBV-related patients preceded with liver cirrhosis, pathologically identified as early-stage HCC with dysplastic nodules. Liver fibrosis tissues, dysplastic nodules and early HCC tissues from these patients were used to measure DNA methylation. Here, we report significant differences in the DNA methylation spectrum among the three types of tissues. In the early stage of HCC, DNA hypermethylation of tumor suppressor genes is predominant. Additionally, DNA hypermethylation in the early stage of HCC changes the binding ability of transcription factor TP53 to the promoter of tumor suppressor gene ZNF334, and inhibits the expression of ZNF334 at the transcription level. Furthermore, through a series of in vivo and in vitro experiments, we have clarified the exacerbation effect of tumor suppressor gene ZNF334 deletion in the occurrence of HCC. Combined with clinical data, we found that the overall survival and relapse-free survival of patients with high ZNF334 expression are significantly longer. Thus, we partly elucidated a sequential alternation of DNA methylation modification during the occurrence of HCC, and clarified the biological function and regulatory mechanism of the tumor suppressor gene ZNF334, which is regulated by related DNA methylation sites. Our study provides a new target and clinical evidence for the early diagnosis and sheds light on the precise treatment of liver cancer.
Macrophage-mediated anti-tumor immunity against high-risk neuroblastoma
Authors: Tang Xao X. , Shimada Hiroyuki , Ikegaki Naohiko 
Published: May 2022

AbstractNeuroblastoma is the most common extracranial childhood solid tumor. The majority of high-risk neuroblastoma is resistant/refractory to the current high intensity therapy. Neuroblastoma lacks classical HLA Class I expression and exhibits low mutation burden, allowing neuroblastoma cells to evade CD8+ T cell-mediated immunity. Neuroblastoma cells do not express PD-L1, and tumor-associated macrophages are the predominant PD-L1+ cells in the tumor. In this study, we performed gene expression profiling and survival analyses on large neuroblastoma datasets to address the prognostic effect of PD-L1 gene expression and the possible involvement of the SLAMF7 pathway in the anti-neuroblastoma immunity. High-level expression of PD-L1 was found significantly associated with better outcome of high-risk neuroblastoma patients; two populations of PD-1+ PD-L1+ macrophages could be present in high-risk tumors with PD-1/PD-L1 ratios, ≈1 and >1. Patients with the PD-1/PD-L1 ratio >1 tumor showed inferior survival. High-level co-expression of SLAMF7 and SH2D1B was significantly associated with better survival of the high-risk neuroblastoma patients. Together, this study supports the hypothesis that macrophages are important effector cells in the anti-high-risk neuroblastoma immunity, that PD-1 blockade therapy can be beneficial to the high-risk neuroblastoma subset with the PD-1/PD-L1 expression ratio >1, and that SLAMF7 is a new therapeutic target of high-risk neuroblastoma.
Inheritance through the cytoplasm
Authors: Camus M. Florencia , Alexander-Lawrie Bridie , Sharbrough Joel , Hurst Gregory D. D. 
Published: May 2022

AbstractMost heritable information in eukaryotic cells is encoded in the nuclear genome, with inheritance patterns following classic Mendelian segregation. Genomes residing in the cytoplasm, however, prove to be a peculiar exception to this rule. Cytoplasmic genetic elements are generally maternally inherited, although there are several exceptions where these are paternally, biparentally or doubly-uniparentally inherited. In this review, we examine the diversity and peculiarities of cytoplasmically inherited genomes, and the broad evolutionary consequences that non-Mendelian inheritance brings. We first explore the origins of vertical transmission and uniparental inheritance, before detailing the vast diversity of cytoplasmic inheritance systems across Eukaryota. We then describe the evolution of genomic organisation across lineages, how this process has been shaped by interactions with the nuclear genome and population genetics dynamics. Finally, we discuss how both nuclear and cytoplasmic genomes have evolved to co-inhabit the same host cell via one of the longest symbiotic processes, and all the opportunities for intergenomic conflict that arise due to divergence in inheritance patterns. In sum, we cannot understand the evolution of eukaryotes without understanding hereditary symbiosis.
Petermann ice shelf may not recover after a future breakup
Authors: Åkesson Henning , Morlighem Mathieu , Nilsson Johan , Stranne Christian , Jakobsson Martin 
Published: May 2022

AbstractFloating ice shelves buttress inland ice and curtail grounded-ice discharge. Climate warming causes melting and ultimately breakup of ice shelves, which could escalate ocean-bound ice discharge and thereby sea-level rise. Should ice shelves collapse, it is unclear whether they could recover, even if we meet the goals of the Paris Agreement. Here, we use a numerical ice-sheet model to determine if Petermann Ice Shelf in northwest Greenland can recover from a future breakup. Our experiments suggest that post-breakup recovery of confined ice shelves like Petermann’s is unlikely, unless iceberg calving is greatly reduced. Ice discharge from Petermann Glacier also remains up to 40% higher than today, even if the ocean cools below present-day temperatures. If this behaviour is not unique for Petermann, continued near-future ocean warming may push the ice shelves protecting Earth’s polar ice sheets into a new retreated high-discharge state which may be exceedingly difficult to recover from.
Colossal barocaloric effects with ultralow hysteresis in two-dimensional metal–halide perovskites
Authors: Seo Jinyoung , McGillicuddy Ryan D. , Slavney Adam H. , Zhang Selena , Ukani Rahil , Yakovenko Andrey A. , Zheng Shao-Liang , Mason Jarad A. 
Published: May 2022

AbstractPressure-induced thermal changes in solids—barocaloric effects—can be used to drive cooling cycles that offer a promising alternative to traditional vapor-compression technologies. Efficient barocaloric cooling requires materials that undergo reversible phase transitions with large entropy changes, high sensitivity to hydrostatic pressure, and minimal hysteresis, the combination of which has been challenging to achieve in existing barocaloric materials. Here, we report a new mechanism for achieving colossal barocaloric effects that leverages the large volume and conformational entropy changes of hydrocarbon order–disorder transitions within the organic bilayers of select two-dimensional metal–halide perovskites. Significantly, we show how the confined nature of these order–disorder phase transitions and the synthetic tunability of layered perovskites can be leveraged to reduce phase transition hysteresis through careful control over the inorganic–organic interface. The combination of ultralow hysteresis and high pressure sensitivity leads to colossal reversible isothermal entropy changes (>200 J kg−1 K−1) at record-low pressures (<300 bar).
Double-tap gene drive uses iterative genome targeting to help overcome resistance alleles
Authors: Bishop Alena L. , López Del Amo Víctor , Okamoto Emily M. , Bodai Zsolt , Komor Alexis C. , Gantz Valentino M. 
Published: May 2022

AbstractHoming CRISPR gene drives could aid in curbing the spread of vector-borne diseases and controlling crop pest and invasive species populations due to an inheritance rate that surpasses Mendelian laws. However, this technology suffers from resistance alleles formed when the drive-induced DNA break is repaired by error-prone pathways, which creates mutations that disrupt the gRNA recognition sequence and prevent further gene-drive propagation. Here, we attempt to counteract this by encoding additional gRNAs that target the most commonly generated resistance alleles into the gene drive, allowing a second opportunity at gene-drive conversion. Our presented “double-tap” strategy improved drive efficiency by recycling resistance alleles. The double-tap drive also efficiently spreads in caged populations, outperforming the control drive. Overall, this double-tap strategy can be readily implemented in any CRISPR-based gene drive to improve performance, and similar approaches could benefit other systems suffering from low HDR frequencies, such as mammalian cells or mouse germline transformations.
Genetic and phylogenetic uncoupling of structure and function in human transmodal cortex
Authors: Valk Sofie L. , Xu Ting , Paquola Casey , Park Bo-yong , Bethlehem Richard A. I. , Vos de Wael Reinder , Royer Jessica , Masouleh Shahrzad Kharabian , Bayrak Şeyma , Kochunov Peter , Yeo B. T. Thomas , Margulies Daniel , Smallwood Jonathan , Eickhoff Simon B. , Bernhardt Boris C. 
Published: May 2022

AbstractBrain structure scaffolds intrinsic function, supporting cognition and ultimately behavioral flexibility. However, it remains unclear how a static, genetically controlled architecture supports flexible cognition and behavior. Here, we synthesize genetic, phylogenetic and cognitive analyses to understand how the macroscale organization of structure-function coupling across the cortex can inform its role in cognition. In humans, structure-function coupling was highest in regions of unimodal cortex and lowest in transmodal cortex, a pattern that was mirrored by a reduced alignment with heritable connectivity profiles. Structure-function uncoupling in macaques had a similar spatial distribution, but we observed an increased coupling between structure and function in association cortices relative to humans. Meta-analysis suggested regions with the least genetic control (low heritable correspondence and different across primates) are linked to social-cognition and autobiographical memory. Our findings suggest that genetic and evolutionary uncoupling of structure and function in different transmodal systems may support the emergence of complex forms of cognition.
Redox-homogeneous, gel electrolyte-embedded high-mass-loading cathodes for high-energy lithium metal batteries
Authors: Kim Jung-Hui , Kim Ju-Myung , Cho Seok-Kyu , Kim Nag-Young , Lee Sang-Young 
Published: May 2022

AbstractLithium metal batteries have higher theoretical energy than their Li-ion counterparts, where graphite is used at the anode. However, one of the main stumbling blocks in developing practical Li metal batteries is the lack of cathodes with high-mass-loading capable of delivering highly reversible redox reactions. To overcome this issue, here we report an electrode structure that incorporates a UV-cured non-aqueous gel electrolyte and a cathode where the LiNi0.8Co0.1Mn0.1O2 active material is contained in an electron-conductive matrix produced via simultaneous electrospinning and electrospraying. This peculiar structure prevents the solvent-drying-triggered non-uniform distribution of electrode components and shortens the time for cell aging while improving the overall redox homogeneity. Moreover, the electron-conductive matrix eliminates the use of the metal current collector. When a cathode with a mass loading of 60 mg cm−2 is coupled with a 100 µm thick Li metal electrode using additional non-aqueous fluorinated electrolyte solution in lab-scale pouch cell configuration, a specific energy and energy density of 321 Wh kg−1 and 772 Wh L−1 (based on the total mass of the cell), respectively, can be delivered in the initial cycle at 0.1 C (i.e., 1.2 mA cm−2) and 25 °C.