bluuebunny/crossref_metadata_2025_split huggingface dataset:
The mechanism of Tubificidae in a sludge reduction system with Cr(Ⅵ) stress: an environmentally friendly scavenger
Authors: Lou Juqing , Xu Hui , Jin Hao , Cao Yongqing , Wang Ruyi 
Published: November 2022

Abstract
The treatment of heavy metals in sewage treatment systems has gained more attention with the increase in heavy metal hazards. Tubificidae in sludge reduction has been widely studied; however, little is known about the effect of Tubificidae in the treatment of Cr-containing wastewater. In this study, the mechanism of worms in the sludge reduction system with Cr stress was studied. Predation experiments by worms in a Cr-containing sludge reduction system were conducted to investigate the changes in enzyme activities in the worms under different concentrations of Cr, and the distribution of Cr in the worm sludge reduction reactor was analyzed. The kinetic model of uptake and elimination of Cr in worms was established. The results of SOD, POD and CAT activities indicated that worms had a certain detoxification effect on Cr(Ⅵ). After 28 days of worm predation experiments, the Cr concentrations in worms, sludge and worm feces increased first and then decreased with exposure time, and the proportion of total Cr and Cr(Ⅵ) in the sludge decreased from 71.98% and 42.7–29.18% and 6.82%, respectively. The detoxification mechanism of the worms could be activated with Cr stress, and 63.22% of the Cr(VI) was converted into Cr(III) by the worms. It can be seen that Tubificidae could be considered a good scavenger of environmental Cr(Ⅵ). The hyperbolic model fits the process of Cr uptake and elimination well and can be used as a predictive tool for worm accumulation.
Evaluation of Push-pull technology for pest and soil fertility management on maize in north western Ethiopia.
Authors: Abate Mesfin , Atnafu Getenet , Alemu Berhanu , Molla Abiyot , Gebremariam Gizaw , Alemneh Yentila , Taddese Messeret 
Published: November 2022

Abstract
Aims
The aims of the study were (i) to evaluate the effectiveness of the push-pull technology against stemborer and striga infestation, (ii) to investigate the impact of the push-pull technology on improving grain yield, and (ii) to assess effect of the push-pull technology on soil fertility.
Methods
The study was conducted in 2017 and 2018 cropping seasons in 3 districts in north western Ethiopia. Three farmers from each district were randomly selected for the study. Each farmer had a set of two treatments (plots): a push-pull and maize monocrop treatments. Data were collected on percentage of maize plants damaged by stemborers, number of emerged striga plants, plant height, grain yield, available phosphorus (P), available potassium (K), total nitrogen (TN), organic carbon (OC), organic matter (OM) and bulk density (BD).
Results
There were significant reduction in stemborer damage (2.8%) and striga count (4.1 Striga plants/m2) in the push-pull compared to maize monocrop plots (15.4% and 21.8 striga plants/m2, respectively). Maize plant height (2.34 m) and grain yield (5.3 t/ha) were significantly higher in the push-pull plots as compared to sole crop (1.9 m and 3.0 t/ha, respectively). Similarly, there were significantly higher P (20.06 mg/kg soil), K (406.86 mg/kg soil), TN (2.5 g/kg soil), OC (42.9 g/kg soil), OM (73.8 g/kg soil) and BD (0.92 g/cm3) levels rating from moderate to high fertility status in the push-pull as compared to monocrop plots ( 11.17 mg/kg soil, 347.93 mg/kg soil, 1.6 g/kg soil, 29.8 g/kg soil, 0.95 g/cm3 and 51.2 g/kg soil, respectively) which is rated from low to moderate soil fertility level. Moreover, BD was significantly lower in PPT (0.92 g/cm3) than in MC (0.95 g/cm3) plots.
Conclusions
Therefore it can be concluded that push pull technology is better in improving soil fertility status which results in better grain yield.
Continuing professional development opportunities for Australian endorsed for scheduled medicines podiatrists - what’s out there and is it accessible, relevant, and meaningful? A cross-sectional survey.
Authors: Martin Saraid E. , Graham Kristin , Banwell Helen A. , Johnson Jacinta L. 
Published: November 2022

Abstract
Background
Non-medical prescribing is a valuable strategy to enable equitable access to medications in the context of the increasing demands on health services globally. Australian podiatrists have been able to seek endorsement for scheduled medicines (ESM) for over a decade. This project investigates the perceptions and habits of these podiatrists in meeting the extra CPD requirements associated with their ESM status.
Methods
Australian ESM podiatrists completed an anonymous, online survey capturing demographics; CPD engagement; and self-reflections of CPD activities.
Results
Twenty percent (n = 33) of all Australian ESM registered podiatrists (N = 167) responded to the survey (18 female; mean ESM status 5 years, range 0–28 years). For the previous registration period, 88% (n = 29) completed the mandatory CPD hours, with 65% (n = 20) reporting they had not completed CPD learning goals prior, only retrospectively to this period. Over 80% identified their last ESM CPD activity as accessible, affordable, and could recommend to colleagues. Conversely, 50% or less agreed the activity increased confidence; changed their practice; improved communication skills; or enabled networking. Most respondents (81%, n = 27) indicated improvements should be made to the content, relevance, accessibility, and meaningfulness of CPD. These findings were supported by the open-ended responses.
Conclusions
Our findings suggest ESM podiatrists engage in CPD that is accessible rather than learning goal driven. Concerningly, CPD activities resulted in low translation of learnings to practice. This brings in to question the value of mandatory CPD systems based on minimum hours, rather than meaningfulness.
The Effect of Diurnal Light Cycles on Biohydrogen Production in a Thermosiphon Photobioreactor
Authors: Bosman Catharine Elizabeth , Wyk Petrie van , Pott Robert William McClelland , Bradshaw Steven Martin 
Published: November 2022

Abstract
Hydrogen production via microbial photofermentation shows great promise as a method for sustainable hydrogen production; however, operating costs associated with photofermentative hydrogen production need to be reduced. Costs can be reduced using a passive circulation system like the thermosiphon photobioreactor, and by operating it under natural sunlight. In this study, an automated system was implemented to investigate the effect of diurnal light cycles on the hydrogen productivity and growth of Rhodospeudomonas palustris and on the operation of a thermosiphon photobioreactor, under controlled conditions. Diurnal light cycles, simulating daylight times, were found to reduce hydrogen production in the thermosiphon photobioreactor demonstrating a low production rate of 0.007 mol·m−3·h−1 (± 0.0002 mol·m−3·h−1) as compared to 0.087 mol·m−3·h−1 (± 0.014 mol·m−3·h−1) under continuous illumination. Glycerol consumption as well as hydrogen yield also decreased under diurnal light cycles. Nonetheless, hydrogen production in a thermosiphon photobioreactor under outdoor conditions was demonstrated as possible avenue for further investigation.
Differential expression of Tet family genes and their potential role in regulating skeletal muscle development of Siniperca chuatsi
Authors: Cheng Jia , Zhu Xin , Meng Yangyang , Chen Congyi , Zeng Wei , Pan Yaxiong , Chu Wuying , Zhang Jianshe 
Published: November 2022

Abstract
DNA methylation and demethylation are crucial epigenetic modification and regulation for animal development, and their dynamic changes may affect skeletal muscle development. The ten-eleven translocation (Tet) family proteins are demethylases which are involved in the dynamic changes of DNA methylation. However, the expression pattern of Tet family genes and their role in myogenesis in fish remains unclear. In this study, the temporal and spatial expression profiles of Tet1, Tet2 and Tet3 were assayed with RT-qPCR techniques in Chinese perch, Siniperca chuatsi. The obtained data showed that the expressions of three Tet family genes were differentially expressed at different development stages. Tet1 was expressed low at blastula stage, but highly expressed at gastrula stage, then remained low until hatching. The expressions of Tet2 and Tet3 were significantly increased at late gastrula and kept high expression before hatching stage. At the spatial level, the Tet1 expression was highest in gill tissue, moderate level in brain and slow muscle. Tet2 was similar to that of Tet1 except that it was expressed at a lower level in slow muscle, and Tet3 exhibited a higher expression level in gill and brain, a moderate level in fast muscle. Cosinor analysis turned out that the expression of Tet1 and Tet2 displayed a significant daily rhythm in fast muscle, but Tet3 did not show daily rhythmicity. Inhibiting the activity of Tet1/2 proteins by injecting Bobcat339 significantly reduced the expression of MyoD and MRF4, but not MyoG and Myf5, by which leads to the increase of the number of satellite cells and proliferating myoblasts. Together, the results suggest that Tet1/2 may target to MyoD and MRF4 resulted in DNA demethylation and promote their expression, and therefore stimulate myoblast differentiation.
Physical exercises for premenstrual syndrome in senior high school students: A prospective study
Authors: Chen Zhuo , Imai Kazuhiro , Zhou Xiao 
Published: November 2022

Abstract
Background
Reproductive-aged women experience somatic and affective symptoms of premenstrual syndrome (PMS) which affect their daily life and work, but there are limited tracking data on senior high school-age female students.
Purpose
To investigate the characteristics and incidence of PMS, and to detect the relationship between physical exercise participation and PMS in female students of senior high school.
Method
A prospective study was conducted among senior high school female students aged 14–16 years. The participant was asked to finish two questionnaires. One questionnaire collected information about demographic data and PMS symptoms. PMS was recorded on a daily calendar named Daily Record of Severity of Problems (DRSP) per day. Another questionnaire was the physical exercises participation questionnaire, collecting times of physical education (PE) class participation, exercise times, exercise type participation in morning exercise and recess exercise, times of morning exercise, times of recess exercise per week, recess exercise time, and autonomous exercise participation of students. The data were prospectively recorded for three consecutive months. Odds ratio (OR) with 95% confidence intervals (CI) was performed for results of the multivariate logistic regression analysis model.
Result
233 participants completed the prospective study. 78 (33.5%) participants of all the participants experienced PMS. The incidence of the participants with mild PMS was 20.2% while moderate PMS was 11.6% and severe PMS was 1.7%. The most common somatic symptom was fatigue while the most common affective symptom was can't concentrate. Participants with PE classes less than 2 times per week were 4.43 times (OR: 4.43, 95% CI: 1.18–16.6, p < 0.05) more possible to experience PMS than those with 2 times PE classes per week.
Conclusions
PMS is a frequent phenomenon in senior high school female students. Female students with 2 times PE classes per week show a lower incidence of PMS. This study encouraged senior high school-age females to take more physical exercises per week and could be helpful to create a no-pharmacology coping strategy.
A Multicenter Study Using a Smartwatch, Smartphone, and Wearable Sensors to Assess Early Parkinson’s Disease: Baseline Results of the WATCH-PD Study
Authors: Adams Jamie , Kangarloo Tairmae , Tracey Brian , O'Donnell Patricio , Volfson Dmitri , Latzman Robert , Zach Neta , Alexander Robert , Bergethon Peter , Cosman Joshua , Anderson David , Best Allen , Kostrzebski Melissa , Auinger Peggy , Wilmot Peter , Pohlson Yvonne , Waddell Emma , Jensen-Roberts Stella , Gong Yishu , Kilambi Krishna Praneeth , Herrero Teresa Ruiz , Dorsey E. 
Published: November 2022

Abstract
Digital health technologies can provide continuous monitoring and objective, real world measures of Parkinson’s disease (PD), but have primarily been evaluated in small, single-site studies. In this 12-month, multicenter observational study, we evaluated whether a smartwatch and smartphone application could measure features of early PD. 82 individuals with early, untreated PD and 50 age-matched controls wore research-grade sensors, a smartwatch, and a smartphone while performing standardized assessments in clinic. At home, participants wore the smartwatch for seven days after each clinic visit and completed motor, speech and cognitive tasks on the smartphone every other week. Features derived from the devices, particularly arm swing, proportion of time with tremor, and finger tapping, differed significantly between individuals with early PD and age-matched controls and had variable correlation with traditional assessments. Longitudinal assessments will inform the value of these digital measures for use in future clinical trials.
Copper (II) complex of salicylate phenanthroline induces apoptosis of colorectal cancer cells including oxaliplatin-resistant cells
Authors: Liu Zixin , Fan Limei , Niu Dongqin , Chen Ming , Zhang Weiran , Xu Jinhua , Wang Dong 
Published: November 2022

Abstract
Background: Oxaliplatin is one of the most effective chemotherapy drugs in the treatment of CRC. However, Oxaliplatin resistance still eventually develops, and the drug is associated with serious side effects. In this study, we examined anti-tumor activity and related molecular mechanism of copper (Ⅱ) complex of salicylate phenanthroline [Cu(sal)(phen)] in colorectal cancer (CRC) cells.
Methods: Cell viability was determined by MTS assay and cell proliferation was analyzed by colony formation assay. Cell apoptosis was measured by flowcytometry with Annexin V/PI staining. Reactive Oxygen Species and depolarization of mitochondrial membrane potential were measured with ROS kit and JC-1 kit, respectively. The anti-tumor effect of Cu(sal)(phen) in vivo was investigated using the HCT116 cell xenograft model.
Results: Cu(sal)(phen) inhibited the growth of colorectal cancer cell lines HCT116 and SW480. Compared to the chemotherapy drug oxaliplatin, Cu(sal)(phen) was more effective at inducing apoptosis, ROS production, and decreased mitochondrial membrane potential in two CRC cell lines HCT116 and SW480. Our western blot showed that the expression of the apoptosis-related proteins Bcl-2 and Survivin as well as upstream regulators p-JAK2 and p-STAT5 were significantly reduced in HCT116 and SW480 after treatment with Cu(sal)(phen). This suggests that Cu(sal)(phen) induces apoptosis of CRC cells via suppressing the JAK2/STAT5 signaling pathway. In vivo, Cu(sal)(phen) inhibited the growth of HCT116 xenografted tumor as compared with the control group. Immunohistochemical results showed that the expression levels of Bcl-2, Survivin, and Ki-67 in tumor tissues were decreased after Cu(sal)(phen) treatment.
Conclusions: Cu(sal)(phen) inhibits CRC cells effectively and may have the potential to be developed as a therapeutic drug for CRC
Experimental Investigation of soiling impact on PV Module Performance in Yanbu Al Sinaiyah, Saudi Arabia
Authors: AlZahrani Khaled S. 
Published: November 2022

Abstract
An area of growing attention is the justification of the environmental effects of clean energy technologies. For many years, photovoltaic modules have been employed extensively in both small- and large-scale applications. They are still not cost-effective compared to other power generation technologies, particularly in an industrial city and the harsh environmental conditions of the coastal desert areas. This study is directed to examine the effects of dust, sand grains, and soiling characterization on the efficiency of PV modules under different environmental conditions. PV modules of two technologies (Mono-Si and poly-Si), two different inclination angles (10° and 24°), and two different surface conditions (clean and soiled) are experimentally tested for concluding and comparing the performance change of PV modules. The main findings imply that, the overall losses of generated power due to soiling effect reached 24%. Mono-Si PV modules were found better than the Poly-Si PV modules under normal conditions, but Poly-Si PV modules were found better in handling the soiling impact. The 10° Inclination angle allows higher performance and better resistance to environmental conditions. Dust characterization study confirmed the effect of the geographical area on the quality and adhesion strength of the accumulated dust particles on the PV modules' surfaces.
Assessment of Comprehensiveness of Medicare Part B Provider-Level Datasets within Dermatology
Authors: Gronbeck Christian , Feng Hao 
Published: November 2022

Abstract
Publicly-available provider-level Medicare Part B datasets have been increasingly utilized for health services research in dermatology. Despite offering detailed insights, an important limitation of these datasets is suppression of services performed for < 11 Medicare beneficiaries at the level of each provider. This longitudinal review assesses the magnitude of this limitation by comparing service counts in provider-level Medicare datasets to those in aggregate datasets, ultimately identifying a concordance rate of 94.3% for dermatology services. However, facility-based visits (52.5%), inpatient evaluation & management visits (59.7%), phototherapy (62.9%), incision & drainage (61.1%), and nail procedures (38.0%) were less well represented in the provider-level datasets. Provider-level datasets are most suitable for assessing dermatology services in aggregate and among specific high-volume procedure groups but alternative datasets should be considered when investigating inpatient services, facility-based services (more common in certain states), or rarely-performed procedures.
Generation of V-point polarization singularity using single phase encoding with a spatial light modulator
Authors: Kumar Praveen , Srinivasa A , Omatsu Takashige 
Published: November 2022

Abstract
A liquid crystal spatial light modulator (SLM) can be used in various ways to produce vector vortices. Superposition of scalar vortices with orthogonal polarization is a common approach, while a more recent technique is to use dual-phase modulation. These approaches require modulation of at least two phase patterns with a SLM or multiple SLMs. In this paper, we propose a novel technique to produce vector-vortices by modulating orthogonal light components through a single phase pattern with a SLM. It does not require interferometric setups, and simplifies the generation of light beams with V-point polarization singularities. Because of compact and robustness of our experimental setup, it can be easily integrated to any device for applications of vector vortices.
FATTY ACID EXPORTER 1 enhances seed oil content in Brassica napus
Authors: Li Yuqing , Ali Usman , Cao Zhouxiao , Zeng Chenghao , Xiao Mengying , Wei Fang , Yao Xuan , Guo Liang , Lu Shaoping 
Published: November 2022

Abstract
FATTY ACID EXPORTER 1 (FAX1) is an initial transporter for fatty acid (FA), in charge of transporting FA from the inside of the plastid to the outside. Brassica napus (B. napus) has nineteen members in the FAX family, of which there are six FAX1 homologous genes. Here, we generated the BnaFAX1 CRISPR mutants (BnaA09.FAX1 and BnaC08.FAX1 were both edited) and overexpression (OE) plants of BnaA09.FAX1 in B. napus. The results showed that the FA content was increased by 0.6–0.9% in OE plant leaves, and the seed oil content was increased by 1.4–1.7% in OE lines, compared to WT. Meanwhile, the content of triacylglycerol, diacylglycerol and phosphatidylcholine was significantly increased in OE seeds. Moreover, seedling biomass and plant height of OE plants were increased compared to WT plants. However, the traits above had no significant difference between the mutants and WT. These results suggest that BnaA09.FAX1 plays a role in improving seed oil accumulation and plant growth, while the function of BnaFAX1 may be compensated by other homologous genes of BnaFAX1 and other BnaFAX genes in the mutants.
Positive Impacts of Leisure-Time Physical Activity on Cardiorespiratory Fitness, Co- Morbidity Level, Cardiovascular Health and Quality of Life among Midlife Adults: A Cross-sectional Study
Authors: Maruf Fatai Adesina , Ucheokoye Demelum Marylyn 
Published: November 2022

Abstract
Background
Regular physical activity (PA) improves general health and quality of life (QoL) of the general population. It is however not known if leisure-time PA (LTPA) behaviour will reduce co-morbidity and adiposity, and improve cardiorespiratory fitness and QoL in midlife men. This study explored the impacts of regular LTPA behaviour on co-morbidity, adiposity, cardiorespiratory fitness and QoL among Nigerian sports club members.
Methods
This cross-sectional study involved 174 age-matched male midlife adults: 87 engaging in LTPA (LTPA group) and 87 not engaging in LTPA (non-LTPA group). Information on age, body mass index (BMI), waist circumference (WC), maximal oxygen uptake (VO2max), resting heart rate (RHR), QoL and co-morbidity level was collected using standardized procedures. Data were summarized using mean and standard deviation, and explored using frequency and proportion. Independent t-test, Chi Square and Mann-Whitney U test were employed to determine the impacts of LTPA at 0.05 significance level.
Results
The LTPA group had lower co-morbidity score (p = 0.05) and RHR (p = 0.004), and higher QoL (p = 0.01) and VO2max (p = 0.003) than non-LTPA group. While heart disease (χ2 = 10.99; p = 0.01) and hypertension (χ2 = 15.24; p = 0.004) severity levels were associated with LTPA behaviour, hypertension (p = 0.01) was the only co-morbid condition that had a significantly lower score in the LTPA group than in the non-LTPA group.
Conclusions
Regular LTPA improves cardiovascular health, physical work capacity and QoL in mid-life men. Regular LTPA behaviour is recommended for cardiovascular health promotion, and improved physical work capacity and life satisfaction in midlife men.