bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictors and prognosis of PCI-related myocardial injury in chronic total occlusion
Authors: Kong Tianzhong , Dai Xintong , Luan Bo , Zhang Xiaojiao , Hou Aijie , Wang Yong 
Published: May 2022

Abstract
Background: Periprocedural myocardial injury (PMI) is associated with major adverse cardiovascular events (MACE) after percutaneous coronary intervention (PCI). However, its incidence and prognostic impact in chronic total occlusion (CTO) PCI remains unclear. Method: To evaluate the predictors and prognostic impact of PMI following PCI in patients with CTO. This study included 132 patients; of them, 8 in whom procedure failed were excluded. Thus, a total of 124 patients in whom procedure was successful were divided into the PMI group (n=42) and the non-PMI group (n=82) according to their c-TnI levels measured hourly after procedure. By comparing the baseline data and angiographic characteristics of the two groups, we analyzed PMI predictors and the correlation between PMI and in-hospital major adverse cardiac events. Results: Overall, PMI occurred in 42 patients (33.9%). Comparing with non-PMI group, PMI group had more diabetes (54.8% vs 31.7%,P=0.013) and dyslipidemia (54.8% vs 13.4%, P＜0.001). Also, there were significant differences between the two groups in left ventricular ejection fraction(43.2±7.2vs47.2±8.0，P=0.027), prior myocardial infarction(54.8%vs43.1%，P=0.020), prior PCI(57.1%vs22.0%，P＜0.001) and prior CABG(14.3%vs2.4%，P=0.011). In angiographic characteristic, patients with PMI had more calcified lesions (52.4%vs24.4%，P=0.002) and were more likely to have multivessel artery disease (71.4%vs35.4%，P＜0.001). In addition, patients in the PMI group had higher J-CTO scores (3.3±1.0vs1.9±0.5，P＜0.001) and were more likely to have wire-crossing difficulties (64.3%vs37.8%，P=0.005), require the use of retrograde approach (38.1%vs7.3%，P＜0.001) and have procedural complications (19.0%vs2.4%，P=0.003). In the multivariate analysis, multivessel artery disease (odd ratio [OR]，4.347；confidence interval [CI]，1.601-11.809；P=0.004), retrograde approach (OR，4.036；CI，1.162-14.020；P=0.028) and the presence of procedural complications (a composite of iatrogenic coronary artery dissection/haematoma/perforation or cardiac tamponade)(OR，16.480；CI，2.515-107.987；P=0.003) were predictors of PMI. Conclusion: The incidence of PMI in CTO patients after PCI was about 33%. Multivessel artery disease, retrograde approach, and the presence of procedural complications were predictors of PMI after CTO-PCI. Patients who develop PMI tend to have a poorer clinical prognosis and more in-hospital MACE than those who do not develop PMI.
Association of plasma brain-derived neurotrophic factor levels and frailty in community- dwelling older adults: The Korean Frailty and Aging Cohort Study
Authors: Roh Eun , Hwang Soon Young , Song Eyun , Park Min Jeong , Yoo Hye Jin , Baik Sei Hyun , Kim Miji , Won Chang Won , Choi Kyung Mook 
Published: May 2022

Abstract
Brain-derived neurotrophic factor (BDNF), an exercise-induced neurotrophin, is an important factor in memory consolidation and cognitive function. This study evaluates the association between plasma BDNF levels and frailty in community-dwelling older adults. Plasma BDNF levels were analyzed in a total of 302 individuals aged 70–84 years from the Korean Frailty and Aging Cohort Study. There were 30 (9.9%) participants with frailty. They were older and had a higher prevalence of dementia and depression than those without frailty. There were no differences in the proportion of male sex between the frail and non-frail groups. Plasma BDNF levels were significantly lower in participants with frailty than in those without frailty. The presence of frailty was significantly associated with plasma BDNF levels (odds ratio 0.508, 95% confidence interval 0.304–0.849) as well as age, hemoglobin, and the presence of dementia, and depression. After adjustment for confounding factors, the significant association between plasma BDNF and frailty was maintained (0.495, 0.281–0.874). This association remained consistent after exclusion of individuals with dementia, depression, stroke, diabetes, and osteoporosis. Plasma BDNF levels were significantly associated with frailty in community-dwelling older adults. Our study may suggest the possible role of BDNF as a novel biomarker of frailty.
Design and Analysis of Hybrid Dispersion Compensation Techniques for 32×40Gbps DWDM Optical Transmission Systems
Authors: GUPTA RAJKUMAR , MEENA M. L. 
Published: May 2022

Abstract
In this article, the proposed modal evaluates the performance of 32×40Gb/s dense wavelength division multiplex (DWDM) optical transmission systems through hybrid dispersion compensation techniques which are Dispersion-Compensation-Fiber (DCF), DCF-FBG (Fiber Braggs Grating), OPC (Optical-Phase-Conjugation)-DCF, and OPC-DCF-FBG using Gaussian, Non-Return-to-Zero (NRZ) and Return-to-Zero (RZ) pulse generators. Performance of proposed modal is evaluated through single-mode fiber (SMF) length between 100-500km with an optimized length of DCF/FBG for low-cost optical transmission systems. The performance of the designed model is evaluated in terms of quality-factor (Qfactor), bit-error-rate (BER), eye diagrams, and cost analysis for all proposed combinations of dispersion compensation techniques. The quality factor and BER are also evaluated at different input power levels. It is found that the hybrid combination of OPC-DCF-FBG techniques having RZ pulse-generator gives the best performance with low cost for 500km long-haul optical transmission systems.
Association of Lipid, Inflammatory and Metabolic Biomarkers With Age at Onset for Incident Cardiovascular Disease
Authors: Tian Xue , Zuo Yingting , Chen Shuohua , Zhang Yijun , Zhang Xiaoli , Xu Qin , Luo Yanxia , Wu Shouling , Wang Anxin 
Published: May 2022

Abstract
Background: Risk profiles for premature cardiovascular disease (CVD) are unclear. This study aimed to examine baseline risk profiles for incident CVD by age at onset in Chinese population. Methods: A total of 97,841 participants without CVD were enrolled from the Kailuan cohort study. Four age groups were examined (<55, 55 to <65, 65 to <75 and ≥75 years) for CVD onset. Risk profiles included clinical, lipid, metabolic, and inflammatory risk factors and biomarkers. Results: Of the clinical factors, diabetes was associated with the highest relative risk for incident CVD in participants younger than 55 years (hazard ratio [HR], 4.12; 95% confidence interval [CI], 3.51-4.84). Risk that were also noted for CVD onset in participants younger than 55 years included hypertension, metabolism syndrome, overweight or obese, dyslipidemia, and smoking. Among the biomarkers, insulin resistance measured by triglyceride-glucose index had the highest HR (1.42; 95% CI, 1.35-1.49) for CVD onset in participants younger than 55 years. In comparison, weaker but significant associations with CVD in participants younger than 55 years were noted for most lipids, metabolic biomarkers, and inflammatory biomarkers. Most risk factors and biomarkers had associations that attenuated with increasing age at onset. Some biomarkers had similar CVD age association, while a few had no association with CVD onset at any age. Conclusions: These findings showed that diabetes and insulin resistance, in addition to hypertension, metabolism syndrome, overweight or obese, dyslipidemia, and smoking, appeared to be the strongest risk factors for premature onset of CVD, and most risk factors had attenuated relative rates at older ages.
Sex differences in Outcomes After Mechanical Thrombectomy for Acute Ischemic Stroke: Real-World Analysis and Literature Review
Authors: Bai Xuesong , Cao Wenbo , Feng Yao , Yu Fan , Fu Zhaolin , Li Wei , Xu Xin , Tian Qiuyue , Guo Xiaofan , Wang Xue , Wang Tao , Sha Araman , Yang Bin , Chen Yanfei , Wang Yabing , Chen Jian , Dmytriw Adam A. , Regenhardt Robert W. , Chen Fei , Ma Qingfeng , Ma Yan , Lu Jie , Gao Peng , Jiao Liqun 
Published: May 2022

Abstract
Objective: To investigate the effect of sex on outcomes after mechanical thrombectomy (MT) for acute ischemic stroke (AIS) in a real-world setting.Methods: AIS patients receiving anterior circulation MT from our tertiary care academic institution were retrospectively reviewed and analyzed. Additionally, a literature review and meta-analysis regarding the impact of sex on MT outcomes was performed. Favorable outcome (90-day mRS 0-2), mortality, intracranial hemorrhage (ICH) and symptomatic ICH (sICH) were compared.Results: 1) From January 2019 to December 2021, a total of 347 AIS patients were recruited, including 126 females and 221 males. Before adjustment, females had a lower rate of favorable outcome (41.3% vs. 52.9%, P=0.037) and a higher rate of mortality (21.3% vs. 11.3%, P=0.867), but the difference was not significant after adjustment. ICH and sICH did not differ comparing females and males. 2) The systematic review and meta-analysis incorporated 8 eligible studies (3129 females and 3383 males), including our original data. A similar phenomenon was demonstrated; females were less likely to have a favorable outcome (OR 0.58, 95%CI 0.45-0.75, I2=77.3% ) and had a higher mortality (20.9% vs. 17.1%, OR 1.24, 95%CI 1.06-1.44, I2=12.4) before adjustment, but there were comparable outcomes after pooling adjusted data (favorable outcome: aOR 0.85, 95%CI 0.71-1.02, I2=63.8%; mortality: aOR 0.85, 95%CI 0.65-1.12, I2=33.6%). There was no significant difference in ICH and sICH.Conclusions: Females showed a worse functional outcome after MT for AIS in a real-world setting, but sex innately is not a predictor of outcome after adjustment by confounding factors.
Combination therapy with bevacizumab and CCR2 inhibitor for women with ovarian cancer: in vivo validation study
Authors: Zhai Tianyue , Mitamura Takashi , Wang Lei , Kubota Shimpei I. , Murakami Masaaki , Tanaka Shinya , Watari Hidemichi 
Published: May 2022

Abstract
Purpose: Anti-angiogenic therapy with bevacizumab (BEV) plays a critical role in the treatment of ovarian cancer. However, despite the initial response, most tumors become resistant to BEV. We examined whether a synergistic effect between BEV and CCR2 inhibitors occurs in patient-derived xenografts (PDXs).Method: We established three consecutive PDXs (first high-grade serous, second clear cell, and third high-grade serous carcinoma) using immunodeficient NOD/Shi-SCID IL-2Rγ KO Jic mice. We randomly assigned mice (n=15, 8, and 8) to receive BEV (10 mg/kg) or a combination of BEV (10 mg/kg) and the CCR2 inhibitor BMS CCR2 22 (low-dose: 20 mg/kg or high-dose: 40 mg/kg) (BEV/CCR2i). Results: Low-dose BEV/CCR2i demonstrated significant growth suppression in the first BEV-resistant PDX and third BEV-sensitive PDX compared with BEV (30.4% after the second cycle and 15.5% after the first cycle, respectively), and treatment cessation did not attenuate this effect. Tissue clearing and immunohistochemistry with an anti-α-SMA antibody suggested that angiogenesis on the tumor surface was suppressed in BEV/CCR2i compared with BEV. Immunohistochemistry of human CD31 demonstrated that the microvessel density originating from human ovarian cancer was significantly lower in BEV/CCR2i than in BEV. Although the second PDX did not respond to low-dose BEV/CCR2i after the fifth cycle, two additional cycles of high-dose BEV/CCR2i demonstrated significant growth suppression compared with BEV (28.3%) by inhibiting the CCR2B-MAPK pathway. Conclusions: BEV/CCR2i showed a sustained and antitumor immunity-independent synergistic effect in human ovarian cancer. This benefit is more significant in serous carcinoma than in clear cell carcinoma.
Development and Validation of a Multivariable Predictive nomogram for Diagnosis of Neonatal Bacterial Meningitis
Authors: Liu Jia , Zhang Shu-Heng , Liu Xiao-Chen , You Ting , Huang Dan , Li Lu-Quan 
Published: May 2022

Abstract
Purpose To develop and validate a multivariable predictive nomogram to diagnose neonatal bacterial meningitis (NBM).Methods A retrospective study was conducted and neonates with positive blood culture were included. Infants were divided into training and validation set. A predictive nomogram was established according to finding of univariate analysis and multivariate logistic regression. The diagnostic value was judged by receiver operating characteristic curves (ROC). ResultsAmong the 587 infants included finally, 177 of them developed NBM. Among training set (N=410), the incidence of abnormal temperature, seizure, bulging anterior fontanel were more common in NBM group (P<0.05). Higher of protein, WBC and lower glucose in cerebrospinal fluid (CSF) were found in NBM group (P<0.05). Bulging anterior fontanel, seizure, abnormal temperature, abnormal protein and WBC in CSF were considered as the independent predictors of NBM. A predictive nomogram was established, the C index was 0.89 and the sensitivity, specificity, positive predictive value, and negative predictive value were 63.4%, 94.3%, 83.3%, and 84.6% in internal validation.The model was validated in validation set (N=177). And the C index was 0.93, and the sensitivity, specificity, positive predictive value, and negative predictive value were 58.7%, 97.7%, 90.0%, and 87.1% in the validation set respectively in external validation. High score would increase the probability of NBM.ConclusionThe model including clinical manifestations had high potential diagnostic value in the prediction of NBM.
Rare but Marvelous Efficacy of Neoadjuvant Chemotherapy for Adenocarcinoma of Rectum: A Case Report
Authors: Zhao Zongxian , Yao Yuan , Wang Fusheng 
Published: May 2022

Abstract
Background: The neoadjuvant chemotherapy (NACT) has been proved to reduce tumor early micro-metastatic focus, reduce tumor stage and increase probability of sparing sphincter, and even decrease risk of recurrence in locally advanced rectal cancer (LACR). Here, we reported a case of locally advanced rectal cancer that was rare extremely sensitive to NACT and reached pathological complete response (pCR) after only 3 courses of preoperative chemotherapy with CAPOX.Case Presentation: A married 60-year-old male was diagnosed with locally advanced rectal cancer (cT3N2). He achieved pathological complete response after three courses of preoperative neoadjuvant chemotherapy with CAPOX. There were no clinically significant adverse reactions during entire chemotherapy period. Until now (February, 2022), the patient was still alive without any signs or symptoms of tumor recurrence.Conclusion: In conclusion, we documented an extremely rare case of LARC that developed pCR after only preoperative 3 courses of CAPOX. In addition, we provided a recommendation to employ early genetic detection to guide preoperative chemotherapy protocol. We should select appropriate NACT regimens according to different genotype of LARC and evaluate the effect of chemotherapy in time.
Expeller-pressed pomegranate seed (Punica granatum L.) as a protein source for the production of antioxidant peptides
Authors: Rahimipanah Maryam , SadeghiMahoonak Alireza , Ghorbani Mohammad , Tabarestani Hoda Shahiri , Nabimeybodi Mohsen 
Published: May 2022

Abstract
Because of health concerns regarding synthetic antioxidants, natural antioxidant compounds are being considered by scientists. Bioactive peptides have been shown to have various physiological functions, such as antioxidative activity. Pomegranate seed protein, the by-product of the pomegranate seed oil industry, can be a good source of bioactive peptides. The optimum conditions for enzymatic hydrolysis of pomegranate seed protein with alcalase were determined using a response surface methodology. The influence of different temperatures (45-55 °C), times (30-180 min), and enzyme to substrate (E/S) ratio (1-3% w/w) on DPPH scavenging power and ferric reducing activity as the responses, were studied. Also, the degree of hydrolysis and the surface hydrophobicity of samples were determined. Moreover, using scanning electron microscopy and electrophoresis technique, microscopic structure and molecular weight of hydrolysate, were studied respectively. Alcalase-derived hydrolysates showed a DPPH scavenging activity (88 ± 0.97 %) and ferric reducing power (0.5 ± 0.83) at optimum conditions of hydrolysis (48.8°C, 97.5 min, E/S ratio 1.3%w/w). The degree of hydrolysis coincided with 36 ±1.2%. An increase in the surface hydrophobicity of the protein during hydrolysis confirmed the unfolding of the pomegranate seed protein structure. The presence of low-molecular-weight peptides was evidenced by the electrophoresis technique. As well as the SEM showed that protein fragments had been reduced to small sizes following enzymatic treatments. According to the results of this study, pomegranate seed protein hydrolysate can be considered a suitable source of antioxidants with an aggregate market value in food formulations
Prognostic impact of copy number alterations in pediatric B-cell precursor acute lymphoblastic leukemia: A collaborative data from two major oncology centers of North India
Authors: Gupta Sanjeev , Singh Minu , HC Pragna , Bakhshi Sameer , Trehan Amita , Gupta Ritu , Thakur Rozy , Gajendra Smeeta , Sharma Preity , Sreedharanu Sreejesh , Sachdeva Man Updesh Singh , Pushpam Deepam , Varma Neelam , Bansal Deepak , Jain Richa , peyam srinivasan , Moorman Anthony , Bhatia Prateek 
Published: May 2022

Abstract
In current study, copy number alteration (CNA) status and CNA risk profiles of IKZF1plus, UK-ALL CNA risk groups and MRplus score, were evaluated for clinical and prognostic impact in a cohort of 493 B-ALL cases diagnosed and treated under ICICLE trial at two major oncology centres of Northern India. Overall CNA frequency was 59% with 60% of cases showing 2-loci deletion. CDKN2A/B deletion was the most common CNA (36.3%), while IKZF1 deletion and IKZF1plus profile were noted in 19.5% and 13.4% of cases respectively. IKZF1 deletions and other CNA risk profiles were all signifcantly associated with poor/high risk clinical and genetic profile parameters (p-<0.001). In addition, the 3-year OS, EFS was significantly poor with high RR of 38.6%, 46.5% and 35.2% for IKZF1 deletions, IKZF1plus profile and UK-ALL CNA –IR+PR risk group respectively (p-<0.001). Integrated evaluation of UK-ALL CNA risk profile with ICICLE trial risk stratification groups also revealed a worse OS, EFS and RR of 63.3%, 43.2% and 35.2% for combined ICICLE groups with CNA-IR+PR profile compared to CNA-GR profile (81.3%,65.0% and 21.0% ; p-<0.001). Hence, routine CNA testing in our setting is must to identify SR and IR cases likely to benefit from high risk treatment.
KAT8 promotes the resistance of human NSCLC cells to miR-7 intervention via the H4K16/PAX6 axis
Authors: Yang Jing , Zhao Xu , Li Dongmei , Tang Lin , Zheng Wen , Chen Longqinq , Chen Jing , Chen Chao , Qin Ming , Guo Mengmeng , Zhou Ya , Zhao Juanjuan , He Zhixu , Xu Lin 
Published: May 2022

Abstract
BackgroundAccumulating evidence has shown that microRNAs (miRNAs), one of the representative types of noncoding RNAs (ncRNAs), contribute to the development of non-small-cell lung cancer (NSCLC) and act as promising therapeutic targets in NSCLC. However, how to improve the efficiency of miRNA-based gene intervention against NSCLC remains largely unknown.MethodsA proliferation assay was used to assess the effect of miR-7 intervention on human NSCLC cells. RNA-seq was performed to analyze the global gene expression of miR-7-resistant cells. Mechanistically, coimmunoprecipitation (Co-IP), chromatin immunoprecipitation-polymerase chain reaction (ChIP-PCR) and western blotting were performed.ResultsUnder continuous intervention with miR-7, miR-7 levels in human NSCLC cells steadily increased, while its inhibition efficiency gradually decreased. The growth and metastatic capacity of miR-7-resistant human NSCLC cells displaying elevated activation of related signaling pathways were significantly enhanced in vitro and in vivo. Global gene analysis showed that the expression of paired box 6 (PAX6), a target molecule of miR-7, was obviously increased in these cells. Moreover, PAX6 silencing significantly impaired the aggressive behavior of miR-7-resistant human NSCLC cells, accompanied by decreased transduction of the PI3K/AKT pathway. Mechanistically, the histone acetyltransferase KAT8 regulated PAX6 expression via H4K16 acetylation, subsequently resulting in the activation of the PI3K/AKT pathway. Notably, PAX6 silencing significantly improved miR-7 intervention efficiency in human NSCLC cells in vitro and in vivo. Finally, the relatively higher miR-7 expression was correlated with PAX6 expression in clinical NSCLC tumors.ConclusionNSCLC cells acquired resistance to miR-7 intervention, orchestrated by KAT8 regulating PAX6 expression via H4K16 acetylation. Specifically, PAX6 silencing significantly improved miR-7 intervention efficiency in human NSCLC cells, thereby providing a feasible approach for miRNA-based intervention in NSCLC.
WITHDRAWN: Mental health and disability research priorities and capacity needs in Ghana: findings from a scoping review and research priority ranking survey.
Authors: Weobong Benedict , Ae-Ngibise Kenneth A. , Mwangi Grace , Sakyi Lionel , Lund Crick 
Published: May 2022

Abstract
Background
Identification of national research agendas for mental health and disability can be supported by well-designed research priority-setting studies. Few Low and Middle-Income Countries (LMICs) have undertaken such studies.
Methods
A mixed methods study comprising a scoping review, research priority ranking survey, and research capacity needs assessment survey was employed to identify mental health and disability research priorities in Ghana. Five expert pools were identified from online search and existing database on mental health civil society organisations/non-governmental organisations. The research priority ranking was completed in two stages, using the Child and Nutrition Research Initiative (CHNRI) method to identify priority questions in stage two. Both surveys were deployed online using google forms. Analysis for the ranking survey involved computing total scores from the CHNRI criteria and generating ranks for the research questions.
Results
68 experts (97% response rate), generated 94 and 92 questions for the short and long-term respectively. 40 experts (58% response rate) completed the ranking stage. The top 10 ranked research questions included: 4 questions addressing mental health systems; 2 questions on epidemiology; and 4 questions on interventions. All research questions were considered urgent and should be conducted in the immediate to short-term (0–5 years). Capacity for disability and mental health research is weak.
Conclusion
Our approach has generated an agenda for mental health and disability research priorities for Ghana and demonstrated that it is feasible to employ a systematic methodology for research priority setting that includes key parameters of context and research capacity.