bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term outcomes at 24- and 36-month follow-up in the intervention arm of the randomized controlled trial of Prompt Mental Health Care
Authors: Smith Otto R. F. , Sæther Solbjørg M. M. , Haug Ellen , Knapstad Marit 
Published: September 2022

Abstract
Background
Whether long-term symptom improvement is maintained after treatment in services such as the Norwegian Prompt Mental Health Care (PMHC) and the English Improving Access to Psychological Therapies is not yet known. In this prospective study, we investigate whether improvements observed at 6-month follow-up are maintained at 24- and 36-month follow-up among clients who received PMHC.

Method
Data from the treatment arm of the randomized controlled trial of PMHC were used (n = 459). The main outcomes were (reliable) recovery rate and symptoms of depression (PHQ-9) and anxiety (GAD-7). Primary outcome data at 24- and 36-months follow-up were available for 47% and 39% of participants, respectively. Secondary outcomes were work participation, functional status, health-related quality of life, and positive mental well-being. Sensitivity analyses with regard to missing data assumptions were conducted for the primary continuous outcomes.

Results
Improvements were maintained at 24- and 36-month follow-up for symptoms of depression and anxiety, (reliable) recovery rate, and health-related quality of life. Small linear improvements since 6-month follow-up were observed for work participation, functional status, and positive mental well-being. Sensitivity analyses did not substantially alter the findings for symptoms of depression and anxiety mentioned above.

Conclusions
Our findings support the long-term effectiveness of PMHC, but results should be interpreted with caution due to lacking follow-up data at 24- and 36-month in the control group, and substantial attrition.
Production of surfactant-stable keratinase from Bacillus cereus YQ15 and its application as detergent additive
Authors: Zhang Rong-Xian , Wu Zhong-Wei , Cui Hai-Yang , Chai Ying-Nan , Hua Cheng-Wei , Wang Peng , Li Lan , Yang Tian-You 
Published: September 2022

Abstract
Background
With the growing concern for the environment, there are trends that bio-utilization of keratinous waste by keratinases could ease the heavy burden of keratinous waste from the poultry processing and leather industry. Especially surfactant-stable keratinases are beneficial for the detergent industry. Therefore, the production of keratinase by Bacillus cereus YQ15 was improved; the characterization and use of keratinase in detergent were also studied.

Results
A novel alkaline keratinase-producing bacterium YQ15 was isolated from feather keratin-rich soil and was identified as Bacillus cereus. Based on the improvement of medium components and culture conditions, the maximum keratinase activity (925 U/mL) was obtained after 36 h of cultivation under conditions of 35 °C and 160 rpm. Moreover, it was observed that the optimal reacting temperature and pH of the keratinase are 60 °C and 10.0, respectively; the activity was severely inhibited by PMSF and EDTA. On the contrary, the keratinase showed remarkable stability in the existence of the various surfactants, including SDS, Tween 20, Tween 60, Tween 80, and Triton X-100. Especially, 5% of Tween 20 and Tween 60 increased the activity by 100% and 60%, respectively. Furtherly, the keratinase revealed high efficiency in removing blood stains.

Conclusion
The excellent compatibility with commercial detergents and the high washing efficiency of removing blood stains suggested its suitability for potential application as a bio-detergent additive.
The hypoxia-driven crosstalk between tumor and tumor-associated macrophages: mechanisms and clinical treatment strategies
Authors: Bai Ruixue , Li Yunong , Jian Lingyan , Yang Yuehui , Zhao Lin , Wei Minjie 
Published: September 2022

AbstractGiven that hypoxia is a persistent physiological feature of many different solid tumors and a key driver for cancer malignancy, it is thought to be a major target in cancer treatment recently. Tumor-associated macrophages (TAMs) are the most abundant immune cells in the tumor microenvironment (TME), which have a large impact on tumor development and immunotherapy. TAMs massively accumulate within hypoxic tumor regions. TAMs and hypoxia represent a deadly combination because hypoxia has been suggested to induce a pro-tumorigenic macrophage phenotype. Hypoxia not only directly affects macrophage polarization, but it also has an indirect effect by altering the communication between tumor cells and macrophages. For example, hypoxia can influence the expression of chemokines and exosomes, both of which have profound impacts on the recipient cells. Recently, it has been demonstrated that the intricate interaction between cancer cells and TAMs in the hypoxic TME is relevant to poor prognosis and increased tumor malignancy. However, there are no comprehensive literature reviews on the molecular mechanisms underlying the hypoxia-mediated communication between tumor cells and TAMs. Therefore, this review has the aim to collect all recently available data on this topic and provide insights for developing novel therapeutic strategies for reducing the effects of hypoxia.
Systematic review of accelerometer-based methods for 24-h physical behavior assessment in young children (0–5 years old)
Authors: Lettink Annelinde , Altenburg Teatske M. , Arts Jelle , van Hees Vincent T. , Chinapaw Mai J. M. 
Published: September 2022

Abstract
Background
Accurate accelerometer-based methods are required for assessment of 24-h physical behavior in young children. We aimed to summarize evidence on measurement properties of accelerometer-based methods for assessing 24-h physical behavior in young children.

Methods
We searched PubMed (MEDLINE) up to June 2021 for studies evaluating reliability or validity of accelerometer-based methods for assessing physical activity (PA), sedentary behavior (SB), or sleep in 0–5-year-olds. Studies using a subjective comparison measure or an accelerometer-based device that did not directly output time series data were excluded. We developed a Checklist for Assessing the Methodological Quality of studies using Accelerometer-based Methods (CAMQAM) inspired by COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN).

Results
Sixty-two studies were included, examining conventional cut-point-based methods or multi-parameter methods. For infants (0—12 months), several multi-parameter methods proved valid for classifying SB and PA. From three months of age, methods were valid for identifying sleep. In toddlers (1—3 years), cut-points appeared valid for distinguishing SB and light PA (LPA) from moderate-to-vigorous PA (MVPA). One multi-parameter method distinguished toddler specific SB. For sleep, no studies were found in toddlers. In preschoolers (3—5 years), valid hip and wrist cut-points for assessing SB, LPA, MVPA, and wrist cut-points for sleep were identified. Several multi-parameter methods proved valid for identifying SB, LPA, and MVPA, and sleep.
Despite promising results of multi-parameter methods, few models were open-source. While most studies used a single device or axis to measure physical behavior, more promising results were found when combining data derived from different sensor placements or multiple axes.

Conclusions
Up to age three, valid cut-points to assess 24-h physical behavior were lacking, while multi-parameter methods proved valid for distinguishing some waking behaviors. For preschoolers, valid cut-points and algorithms were identified for all physical behaviors. Overall, we recommend more high-quality studies evaluating 24-h accelerometer data from multiple sensor placements and axes for physical behavior assessment. Standardized protocols focusing on including well-defined physical behaviors in different settings representative for children’s developmental stage are required. Using our CAMQAM checklist may further improve methodological study quality.

PROSPERO Registration number
CRD42020184751.
A humanized 4-1BB-targeting agonistic antibody exerts potent antitumor activity in colorectal cancer without systemic toxicity
Authors: Cheng Lian-sheng , Cheng Yong-feng , Liu Wen-ting , Shen Aolin , Zhang Dayan , Xu Tingjuan , Yin Wu , Cheng Min , Ma Xiaopeng , Wang Fengrong , Zhao Qun , Zeng Xiaoli , Zhang Yan , Shen Guodong 
Published: September 2022

Abstract
Background
Colorectal cancer (CRC) is one of the most common malignancies and the patient survival rate remains unacceptably low. The anti-programmed cell death-1 (PD-1)/programmed cell death ligand 1 (PD-L1) antibody-based immune checkpoint inhibitors have been added to CRC treatment regimens, however, only a fraction of patients benefits. As an important co-stimulatory molecule, 4-1BB/CD137 is mainly expressed on the surface of immune cells including T and natural killer (NK) cells. Several agonistic molecules targeting 4-1BB have been clinically unsuccessful due to systemic toxicity or weak antitumor effects. We generated a humanized anti-4-1BB IgG4 antibody, HuB6, directed against a unique epitope and hypothesized that it would promote antitumor immunity with high safety.

Methods
The antigen binding specificity, affinity and activity of HuB6 were determined by enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR), biolayer interferometry (BLI) and flow cytometry. The antitumor effects were evaluated in humanized mice bearing syngeneic tumors, and possible toxicity was evaluated in humanized mice and cynomolgus monkeys.

Results
HuB6 showed high specificity and affinity for a binding epitope distinct from those of other known 4-1BB agonists, including utomilumab and urelumab, and induced CD8 + T, CD4 + T and NK cell stimulation dependent on Fcγ receptor (FcγR) crosslinking. HuB6 inhibited CRC tumor growth in a dose-dependent manner, and the antitumor effect was similar with urelumab and utomilumab in humanized mouse models of syngeneic CRC. Furthermore, HuB6 combined with an anti-PD-L1 antibody significantly inhibited CRC growth in vivo. Additionally, HuB6 induced antitumor immune memory in tumor model mice rechallenged with 4 × 106 tumor cells. Toxicology data for humanized 4-1BB mice and cynomolgus monkeys showed that HuB6 could be tolerated up to a 180 mg/kg dose without systemic toxicity.

Conclusions
This study demonstrated that HuB6 should be a suitable candidate for further clinical development and a potential agent for CRC immunotherapy.
Breastfeeding initiation, duration, and experiences of mothers of late preterm twins: a mixed-methods study
Authors: Jonsdottir Rakel B. , Flacking Renée , Jonsdottir Helga 
Published: September 2022

AbstractBackgroundTwins and late preterm (LPT) infants are at an increased risk of being breastfed to a lesser extent than term singletons. This study aimed to describe the initiation and duration of any and exclusive breastfeeding at the breast for mothers of LPT twins and term twins during the first 4 months and to explore the breastfeeding experiences of mothers of LPT twins.MethodsA sequential two-sample quantitative–qualitative explanatory mixed-methods design was used. The quantitative data were derived from a longitudinal cohort study in which 22 mothers of LPT twins and 41 mothers of term twins answered questionnaires at one and four months after birth (2015–2017). The qualitative data were obtained from semi-structured interviews with 14 mothers of LPT twins (2020–2021), based on results from the quantitative study and literature. Analysis included descriptive statistics of quantitative data and deductive content analysis of the qualitative data, followed by condensation and synthesis.ResultsAll mothers of LPT twins (100%) and most mothers of term twins (96%) initiated breastfeeding. There was no difference in any breastfeeding during the first week at home (98% versus 95%) and at 1 month (88% versus 85%). However, at 4 months, the difference was significant (44% versus 75%). The qualitative data highlighted that mothers of LPT twins experienced breastfeeding as complex and strenuous. Key factors influencing mothers’ experiences and decisions were their infants’ immature breastfeeding behaviors requiring them to express breast milk alongside breastfeeding, the burden of following task-oriented feeding regimes, and the lack of guidance from healthcare professionals. As a result, mothers started to question the worth of their breastfeeding efforts, leading to changes in breastfeeding management with diverse results. Support from fathers and grandparents positively influenced sustained breastfeeding.ConclusionsMothers of LPT twins want to breastfeed, but they face many challenges in breastfeeding during the first month, leading to more LPT twins’ mothers than term twins’ mothers ceasing breastfeeding during the following months. To promote and safeguard breastfeeding in this vulnerable group, care must be differentiated from routine term infant services, and healthcare professionals need to receive proper education and training.
Prevalence of lung atelectasis in sedated dogs examined with computed tomography
Authors: Reimegård Elin , Lee Helena T. Nyman , Westgren Frida 
Published: September 2022

Abstract
Background
Computed tomography (CT) scanning of the lung is known to be a valuable tool when investigating lung pathology of the dog. During CT-scan the dog needs to be immobilized and general anesthesia has historically been considered as gold standard although being a more expensive and time-consuming alternative to sedation. Today, modern high speed multidetector CT-scanners offer new possibilities for sedation as an alternative. Both anesthesia and sedation can cause lung atelectasis, and this can be problematic when reading the CT-images since it potentially can masque or mimic lung pathology leading to misdiagnosis. The objective of this prospective analytic study was to investigate the prevalence of lung atelectasis and changes in lung attenuation over time in dogs that receive intravenous sedation and positioned in sternal recumbency.

Results
20 dogs without known lung pathology underwent three consecutive CT-scans of the lung; the first scan was initiated as soon as the dog was sufficiently sedated, the second scan approximately 5 min after the first one and the last scan after the dog’s orthopaedic scan was completed. The dogs received intravenous sedation in a combination of dexmedetomidine and butorphanol and were kept positioned in a strict sternal recumbency during sedation and exam. Each lung lobe was individually examined in an axial plane and measurements of dorsal, ventral, and mean lung attenuation were made. Atelectasis or areas with poorly aerated lung tissue were not detected as all parts of the lobes were normally aerated at all three scans. A statistically significant increase in lung attenuation between the first and the second scan (P = 0.03) and between the first and the third scan (P = 0.0004) was seen in the ventral part of the lobes.

Conclusions
This study indicates that CT-examination of the lungs can be performed on sedated dogs that are kept in a sternal recumbency without development of atelectasis. It also suggests that there is an early correlation between time and increase in lung attenuation.
Future of industry 5.0 in society: human-centric solutions, challenges and prospective research areas
Authors: Adel Amr 
Published: September 2022

AbstractIndustry 4.0 has been provided for the last 10 years to benefit the industry and the shortcomings; finally, the time for industry 5.0 has arrived. Smart factories are increasing the business productivity; therefore, industry 4.0 has limitations. In this paper, there is a discussion of the industry 5.0 opportunities as well as limitations and the future research prospects. Industry 5.0 is changing paradigm and brings the resolution since it will decrease emphasis on the technology and assume that the potential for progress is based on collaboration among the humans and machines. The industrial revolution is improving customer satisfaction by utilizing personalized products. In modern business with the paid technological developments, industry 5.0 is required for gaining competitive advantages as well as economic growth for the factory. The paper is aimed to analyze the potential applications of industry 5.0. At first, there is a discussion of the definitions of industry 5.0 and advanced technologies required in this industry revolution. There is also discussion of the applications enabled in industry 5.0 like healthcare, supply chain, production in manufacturing, cloud manufacturing, etc. The technologies discussed in this paper are big data analytics, Internet of Things, collaborative robots, Blockchain, digital twins and future 6G systems. The study also included difficulties and issues examined in this paper head to comprehend the issues caused by organizations among the robots and people in the assembly line.
Supplementing the early diet of broilers with soy protein concentrate can improve intestinal development and enhance short-chain fatty acid-producing microbes and short-chain fatty acids, especially butyric acid
Authors: Zhang Qianyun , Zhang Shan , Wu Shu , Madsen Marianne Hjøllund , Shi Shourong 
Published: September 2022

Abstract
Background
Research on nutrition in early-life commonly focuses on the maturation of the intestine because the intestinal system is crucial for ensuring continued growth. To explore the importance of early nutrition regulation in animals, soy protein concentrate (SPC) was added to the early diet of broilers to investigate its effects on amino acid digestibility, intestinal development, especially intestinal microorganisms, and broiler metabolites. A total of 192 one-day-old Arbor Acres (AA) male broilers were randomly assigned to two experimental treatments with 8 replicates of 12 birds. The control group was fed a basal diet (control), and the treatment group was fed a basal diet supplemented with 12% SPC (SPC12) during the first 10 d (starter phase). From d 11 to 21 (grower phase) and d 22 to 42 (finisher phase), a basal diet was fed to both treatment groups.

Results
SPC reduced the pH value and acid-binding capacity of the starter diet (P < 0.05, d 10); SPC in the early diet enhanced the gizzard weight (P < 0.05, d 10 and d 42) and the ileum weight (P < 0.05, d 10) and decreased the weight and length of the jejunum (P < 0.05, d 10) and the relative length of the duodenum and jejunum (P < 0.05, d 10). At the same time, SPC enhanced villus height (P < 0.05, d 10) and muscle thickness in the jejunum and ileum (P < 0.05, d 10) and increased the number of goblet cells in the duodenum (P < 0.05, d 10). Meanwhile, SPC increased the Chao1 index and the ACE index (P < 0.05, d 10) and altered the composition of caecal microflora at d 10. SPC also increased the relative abundance of Alistipes, Anaerotruncus, Erysipelatoclostridium, Intestinimonas and Flavonifractor bacteria (P < 0.05, d 10). At the same time, the concentrations of caecal butyric acid and total short-chain fatty acids (SCFAs) were also increased in the SPC12 group (P < 0.05, d 10).

Conclusions
In summary, the results showed that supplementing the starter diet of broilers with SPC has a significant effect on the early development of the intestine and the microflora.

Graphical abstract
Simulative comparison of concepts for simultaneous control of heat flow and outlet temperature of heat exchangers for highly flexible use in the test facility “District LAB”
Authors: Lottis Dennis , Kallert Anna 
Published: September 2022

AbstractAn important measure for decarbonising the heating sector is the transformation of existing district heating (DH) systems into low-emission heating grids based on renewable heat sources. The test facility for DH applications “District LAB (D-LAB)” is currently being set up in order to support the transition by enabling the experimental investigation of various transformation measures. It consists mainly of a Flexible Heating Grid (FHG), which connects several decentralised Hardware-in-the-Loop (HIL) and is fed by a central generation plant. The HIL units in the FHG should be able to map almost any heat producer or consumer, so that a wide range of investigation scenarios is possible. In order to fulfil this requirement, the Heat Exchanger (HEx) that connects the HIL units with the FHG must extract or feed a defined heat flow from the grid and at the same time ensure a defined secondary-side outlet temperature. In a literature research conducted to the best of our knowledge, it turned out that in all technical applications only one outlet temperature is controlled. Therefore a suitable control concept had to be developed. This has been done by extensive simulative investigations, which are presented in this paper. Two control concepts, (I) “decoupled PID controller”, (II) “Characteristic Field (CF)-Based Control”, were self-developed and compared with each other. In order to represent a large spectrum of possible operating conditions with regard to the transformation of DH systems, 48 investigation scenarios based on target value steps were defined and used for the comparison. Subsequently, the average performance using the Root Mean Square Error (RMSE) as well as the time dependant step responses were examined. It turned out that, the CF-based concept appears to be more suitable for use in the D-LAB. It is conceivable that these results can also be transferred to other test facilities in which both, the heat flow and one outlet temperature need to be controlled.
Max-consensus protocol to determine the regulated node in distributed voltage regulation
Authors: Danner Dominik , de Meer Hermann 
Published: September 2022

AbstractLiterature that deals with optimal voltage regulation in the power distribution grid often neglects the impact of communication on their control strategies or makes hard assumptions about the communication network. This paper extends a nearly optimal reactive power allocation mechanisms for distributed renewable energy sources with communication considerations and proposes a max-consensus-based communication schema to determine the regulated node in a distributed manner. The proposed consensus mechanisms is validated by co-simulation of a multi-agent system, the IEEE 906 low voltage test feeder and a simple communication simulator to evaluate the impact of message delay and communication network topology.