bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATF2 loss promotes tumor invasion in colorectal cancer cells via upregulation of cancer driver TROP2
Authors: Huebner Kerstin , Erlenbach-Wuensch Katharina , Prochazka Jan , Sheraj Ilir , Hampel Chuanpit , Mrazkova Blanka , Michalcikova Tereza , Tureckova Jolana , Iatsiuk Veronika , Weissmann Anne , Ferrazzi Fulvia , Kunze Philipp , Nalli Enise , Sammer Elisabeth , Gehring Annemarie , Cheema Marie M. , Eckstein Markus , Paap Eva-Maria , Soederberg Agnes , Fischer Corinna , Paul Sushmita , Mahadevan Vijayalakshmi , Ndreshkjana Benardina , Meier Melanie A. , Muehlich Susanne , Geppert Carol I. , Merkel Susanne , Grutzmann Robert , Roehe Adriana , Banerjee Sreeparna , Hartmann Arndt , Sedlacek Radislav , Schneider-Stock Regine 
Published: July 2022

AbstractIn cancer, the activating transcription factor 2 (ATF2) has pleiotropic functions in cellular responses to growth stimuli, damage, or inflammation. Due to only limited studies, the significance of ATF2 in colorectal cancer (CRC) is not well understood. We report that low ATF2 levels correlated with worse prognosis and tumor aggressiveness in CRC patients. NanoString gene expression and ChIP analysis confirmed trophoblast cell surface antigen 2 (TROP2) as a novel inhibitory ATF2 target gene. This inverse correlation was further observed in primary human tumor tissues. Immunostainings revealed that high intratumoral heterogeneity for ATF2 and TROP2 expression was sustained also in liver metastasis. Mechanistically, our in vitro data of CRISPR/Cas9-generated ATF2 knockout (KO) clones revealed that high TROP2 levels were critical for cell de-adhesion and increased cell migration without triggering EMT. TROP2 was enriched in filopodia and displaced Paxillin from adherens junctions. In vivo imaging, micro-computer tomography, and immunostainings verified that an ATF2KO/TROP2high status triggered tumor invasiveness in in vivo mouse and chicken xenograft models. In silico analysis provided direct support that ATF2low/TROP2high expression status defined high-risk CRC patients. Finally, our data demonstrate that ATF2 acts as a tumor suppressor by inhibiting the cancer driver TROP2. Therapeutic TROP2 targeting might prevent particularly the first steps in metastasis, i.e., the de-adhesion and invasion of colon cancer cells.
MiR-30c-1-3p targets matrix metalloproteinase 9 involved in the rupture of abdominal aortic aneurysms
Authors: Yang Lin , Sui Hong-Gang , Wang Meng-Meng , Li Jia-Yin , He Xiao-Feng , Li Jing-Yuan , Wang Xiao-Zeng 
Published: July 2022

Abstract
Abdominal aortic aneurysm (AAA) can be fatal if ruptured, but there is no predictive biomarker. Our aim was to evaluate the prognostic potential of microRNAs (miRNAs/miRs) in an AAA mouse model and patients with unruptured AAA (URAAA) and ruptured AAA (RAAA). Among the 64 miRNAs differentially expressed in mice with AAA compared to control, miR-30c-1-3p, miR-432-3p, miR-3154, and miR-379-5p had high homology with human miRNAs. MiR-30c-1-3p plasma levels were significantly lower in patients with RAAA than in those with URAAA or control and tended to negatively correlate with the maximum aortic diameter (r =  −0.3153, P = 0.06109). MiR-30c-1-3p targeted matrix metalloproteinase (MMP)-9 mRNA through the coding region and downregulated its expression in vitro. MMP-9 plasma concentrations were significantly higher in the RAAA group than in the URAAA group (P < 0.001) and were negatively associated with miR-30c-1-3p levels (r =  −0.3671, P = 0.01981) and positively–with the maximal aortic diameter (r = 0.6251, P < 0.0001). The optimal cutoff values for MMP-9 expression and the maximal aortic diameter were 461.08 ng/ml and 55.95 mm, with areas under the curve of 0.816 and 0.844, respectively. Our results indicate that plasma levels of miR-30c-1-3p and MMP-9 may be candidate biomarkers of AAA progression.

Key messages

Downregulation of miR-30c-1-3p expression and upregulation of its potential target MMP-9 are predictors of the devastation of AAA.

Downregulation of miR-30c-1-3p expression and its downstream impact on MMP-9 have a potential on predicting the development and rupture of AAA.
Parenthood and lower risk of suicide in women and men: the total Swedish population followed across adulthood
Authors: Sörberg Wallin Alma , Sjöqvist Hugo , Dehara Marina , Wells Michael B. , Dykxhoorn Jennifer , Kosidou Kyriaki , Dalman Christina 
Published: July 2022

Abstract
Background
Previous studies suggest a protective effect of parenthood on suicide, but little is known about how the association may change across the lifespan, or in relation to sex, marital status or occurrence of psychiatric disorders.

Methods
We followed a cohort of over 5 million Swedish women and men, from 1991 to 2011, up to max. age 75, for death by suicide using national registers. Information on childbirths/adoptions, potential confounders and modifying factors were obtained from national registers. We assessed the associations between parenthood and suicide across adulthood using within time-stratified Cox regression models, with parenthood as a time-dependent exposure.

Results
Parents had a lower risk of suicide than non-parents across the lifespan, after adjusting for sociodemographic factors. The association was most pronounced in young adults, especially young women, but attenuated with increasing age and converged between sexes in older age groups. The lower risk of suicide over the life course was similar whether parents were married, unmarried or divorced, apart from married men; among them, parents only had a lower risk above age 55. The lower risk in parents was also evident in people with a history of psychiatric hospitalizations, but disappeared from age 55 in this population.

Conclusion
The lower risk of suicide was present in both parents, was most pronounced in young adulthood and weakened with increasing age. Our results are consistent with a plausible mechanism where feelings of responsibility and connectedness are protective against suicide in parents.
Chest examinations in children with real-time magnetic resonance imaging: first clinical experience
Authors: Hirsch Franz Wolfgang , Sorge Ina , Voit Dirk , Frahm Jens , Prenzel Freerk , Wachowiak Robin , Anders Rebecca , Roth Christian , Gräfe Daniel 
Published: July 2022

Abstract
Background
Real-time magnetic resonance imaging (MRI) based on a fast low-angle shot technique 2.0 (FLASH 2.0) is highly effective against artifacts caused due to the bulk and pulmonary and cardiac motions of the patient. However, to date, there are no reports on the application of this innovative technique to pediatric lung MRI.

Objective
This study aimed to identify the limits of resolution and image quality of real-time lung MRI in children and to assess the types and minimal size of lesions with these new sequences.

Materials and methods
In this retrospective study, pathological lung findings in 87 children were classified into 6 subgroups, as detected on conventional MRI: metastases and tumors, consolidation, scars, hyperinflation, interstitial pathology and bronchiectasis. Subsequently, the findings were grouped according to size (4–6 mm, 7–9 mm and ≥ 10 mm) and evaluated for visual delineation of the findings (0 = not visible, 1 = hardly visible and 2 = well visualized).

Results
Real-time MRI allows for diagnostic, artifact-free thorax images to be obtained, regardless of patient movements. The delineation of findings strongly correlates with the size of the pathology. Metastases, consolidation and scars were visible at 100% when larger than 9 mm. In the 7–9 mm subgroup, the visibility was 83% for metastases, 88% for consolidation and 100% for scars in T2/T1 weighting. Though often visible, smaller pathological lesions of 4–6 mm in size did not regularly meet the expected diagnostic confidence: The visibility of metastases was 18%, consolidation was 64% and scars was 71%. Diffuse interstitial lung changes and hyperinflation, known as “MR-minus pathologies,” were not accessible to real-time MRI.

Conclusion
The method provides motion robust images of the lung and thorax. However, the lower sensitivity for small lung lesions is a major limitation for routine use of this technique. Currently, the method is adequate for diagnosing inflammatory lung diseases, atelectasis, effusions and lung scarring in children with irregular breathing patterns or bulk motion on sedation-free MRI. A medium-term goal is to improve the diagnostic accuracy of small nodules and interstitial lesions.
Sensory processing and adaptive behavior in Phelan-McDermid syndrome: a cross-sectional study
Authors: Serrada-Tejeda Sergio , Cuadrado María-Luz , Martínez-Piédrola Rosa Mª , Máximo-Bocanegra Nuria , Sánchez-Herrera-Baeza Patricia , Camacho-Montaño Lucía Rocío , Pérez-de-Heredia-Torres Marta 
Published: July 2022

AbstractPhelan-McDermid syndrome (PMS) is a genetic disorder caused by a mutation or deletion of the SHANK3 gene (chromosome 22q13.3), characterized by different sensory processing anomalies. The objective of this study is to expand and provide a detailed definition of the sensory profile of patients with PMS. The secondary objective was to examine the relationship between sensory patterns and adaptive behavior. A cross-sectional study was carried out among 51 Spanish patients with a confirmed genetic diagnosis of PMS. All the participants’ parents completed the Short Sensory Profile-Spanish (SSP-S) and the Adaptive Behavior Assessment System II (ABAS-II). Correlational, multiple regression and hierarchical cluster analyses were performed. An atypical sensory profile was identified in almost 75% of PMS patients. Definite differences were found among scores; nonetheless, sub-threshold values were observed in tactile sensitivity, underresponsive/seeks sensation, auditory filtering, and low energy/weak sensory categories. Conceptual, social, and practical domains, as well as the General Adaptive Composite (GAC) of the ABAS-II showed extremely low scores (i.e., <70). Significant correlations were found (p<0.005) between SSP-S scores and the conceptual, social, practical, and GAC index of the ABAS-II, whereby higher SSP-S scores were associated with better skills and higher adaptive performance. The cluster analysis indicated that the group with the largest mutation size (7.23 Mb) showed the greatest sensory processing difficulties and very low adaptive skills.Conclusions: Patients with PMS show an atypical sensory profile, which correlates with limitations in general adaptive behaviors.

What is Known:• PMS sensory processing difficulties were associated with a pattern of underresponsive/seeks sensation, low energy/weak, and tactile
hyporeactivity.• Sensory processing difficulties have been associated with limitations in the development of appropriate adaptive communication and
interaction behaviors.

What is New:• Sensory definite differences associated with tactile hyperreactivity, as well as significant effects of underresponsiveness/seeks sensation and auditory filtering categories on the adaptive abilities were found in SHANK3deletionpatients.• Cluster analysis suggests that smaller mutation sizes were related to better sensory processing and higher adaptive skills, while patients with larger deletion sizes have greater adaptive difficulties and worse sensory processing skills.
Dysphagia Management and Cervical Auscultation: Reliability and Validity Against FEES
Authors: Jaghbeer Mariam , Sutt Anna-Liisa , Bergström Liza 
Published: July 2022

AbstractThis study investigated the reliability and validity (sensitivity and specificity) of cervical auscultation (CA) using both swallow and pre-post swallow-respiratory sounds, as compared with Flexible Endoscopic Evaluation of Swallowing (FEES). With 103 swallow-respiratory sequences from 23 heterogenic patients, these swallows sounds were rated by eight CA-trained Speech-Language Pathologists (SLPs) to investigate: (1) if the swallow was safe (primary outcome); (2) patient dysphagia status; (3) the influence of liquid viscosity on CA accuracy (secondary outcomes). Primary outcome data showed high CA sensitivity (85.4%), and specificity (80.3%) with all consistencies for the safe measurement, with CA predictive values of $$>$$
>
90% to accurately detect unsafe swallows. Intra-rater reliability was good (Kappa $$=$$
=
0.65), inter rater reliability moderate (Kappa $$=$$
=
0.58). Secondary outcome measures showed high sensitivity (80.1%) to identify if a patient was dysphagic, low specificity (22.9%), and moderate correlation (rs$$=$$
=
0.62) with FEES. A difference across bolus viscosities identified that CA sensitivities (90.1%) and specificities ($$>$$
>
84.7%) for thin liquids were greater than for thick liquids (71.0–77.4% sensitivities, 74.0–81.3% specificities). Results demonstrate high validity and moderate-good reliability of CA-trained SLPs to determine swallow safety when compared with FEES. Data support the use of CA as an adjunct to the clinical swallow examination. CA should include pre-post respiratory sounds and requires specific training. Clinical implications: The authors advocate for holistic dysphagia management including instrumental assessment and ongoing CSE/review $$+$$
+
CA. Adding CA to the CSE/review does not replace instrumental assessment, nor should CA be used as a stand-alone tool.
Early thrombocytopenia is associated with an increased risk of mortality in patients with traumatic brain injury treated in the intensive care unit: a Finnish Intensive Care Consortium study
Authors: Lillemäe Kadri , Luostarinen Teemu , Reinikainen Matti , Bendel Stepani , Laitio Ruut , Hoppu Sanna , Ala-Kokko Tero , Niemi Tomi , Skrifvars Markus B. , Raj Rahul 
Published: July 2022

Abstract
Background
Coagulopathy after traumatic brain injury (TBI) is associated with poor prognosis.

Purpose
To assess the prevalence and association with outcomes of early thrombocytopenia in patients with TBI treated in the intensive care unit (ICU).

Methods
This is a retrospective multicenter study of adult TBI patients admitted to ICUs during 2003–2019. Thrombocytopenia was defined as a platelet count < 100 × 109/L during the first day. The association between thrombocytopenia and hospital and 12-month mortality was tested using multivariable logistic regression, adjusting for markers of injury severity.

Results
Of 4419 patients, 530 (12%) had early thrombocytopenia. In patients with thrombocytopenia, hospital and 12-month mortality were 26% and 48%, respectively; in patients with a platelet count > 100 × 109/L, they were 9% and 22%, respectively. After adjusting for injury severity, a higher platelet count was associated with decreased odds of hospital mortality (OR 0.998 per unit, 95% CI 0.996–0.999) and 12-month mortality (OR 0.998 per unit, 95% CI 0.997–0.999) in patients with moderate-to-severe TBI. Compared to patients with a normal platelet count, patients with thrombocytopenia not receiving platelet transfusion had an increased risk of 12-month mortality (OR 2.2, 95% CI 1.6–3.0), whereas patients with thrombocytopenia receiving platelet transfusion did not (OR 1.0, 95% CI 0.6–1.7).

Conclusion
Early thrombocytopenia occurs in approximately one-tenth of patients with TBI treated in the ICU, and it is an independent risk factor for mortality in patients with moderate-to-severe TBI. Further research is necessary to determine whether this is modifiable by platelet transfusion.
Virtual reality application for real-time pedalling cadence estimation based on hip ROM tracking with inertial sensors: a pilot study
Authors: Rojo Ana , Raya Rafael , Moreno Juan C. 
Published: July 2022

AbstractVirtual reality (VR) applications on rehabilitation a home-base exercise experiences have boomed in the last decade. This is mainly because their entertainment capacity creates a sense of immersion in the users, which enhances adherence to their use. In addition, offering body-related visual feedback is a proven approach to the physical training towards a goal. Recent literature showed the exercise of pedalling has the potential to provide a high number of flexion and extension repetitions of the lower limb in reasonable therapeutic time periods to improve muscle activity, strength and balance in elders, but also motor improvements in patients with neurological injuries. The objective of this work is to present a low-cost wireless application in virtual reality (VR) for pedalling exercises. The platform developed consists of a VR headset and an inertial measurement unit (IMU). The VR headset processes the kinematic information of the IMU to estimate the cadence of the pedalling, while the IMU sensor tracks the angle of hip flexion/extension movement of the user. In order to confirm the suitability of this cadence estimation system, our approach is confronted with a cycling platform developed and validated in a previous study. In the present study, we carried out two repeated sessions with 13 subjects at 3 set speeds: slow (30 rpm), medium (60 rpm) and fast (90 rpm). The Spearman’s correlation (PC) between both systems for the 3 speeds and sessions shows high correlation values for low and medium speeds and moderate correlation for high speed. The SEM results for each system show low measurement error (about 1 cycle) for both systems at every target speed, except for the virtual cycling platform at the highest speed (SEM of VCP at 90 rpm = 3.24 cycles). The repeatability analysis based on ICC (3, 1) absolute agreement shows consistency in all measurements for both systems at high speed and also reflects the irregularity in measurements at low and medium speeds, where participants were less stable during testing due to entertainment from the VR system. All in all, it is concluded the validity of the cadence estimation system for pedalling exercises with low intensity. This development allows us to control the virtual environment by adapting the visual stimulus to cycling cadence. The proposed system can generate sensitive inputs to influence the user’s pedalling cadence.
A prosocial function of head-gaze aversion and head-cocking in common marmosets
Authors: Spadacenta Silvia , Dicke Peter W. , Thier Peter 
Published: July 2022

AbstractGaze aversion is a behavior adopted by several mammalian and non-mammalian species in response to eye contact, and is usually interpreted as a reaction to a perceived threat. Unlike many other primate species, common marmosets (Callithrix jacchus) are thought to have a high tolerance for direct gaze, barely exhibiting gaze avoidance towards conspecifics and humans. Here we show that this does not hold for marmosets interacting with a familiar experimenter who suddenly establishes eye contact in a playful interaction (peekaboo). Video footage synchronously recorded from the perspective of the marmoset and the experimenter showed that the monkeys consistently alternated between eye contact and head-gaze aversion, and that these responses were often preceded by head-cocking. We hypothesize that this behavioral strategy helps marmosets to temporarily disengage from emotionally overwhelming social stimulation due to sight of another individual’s face, in order to prepare for a new round of affiliative face-to-face interactions.
Response of a mesocarnivore community to a new food resource: recognition, exploitation, and interspecific competition
Authors: Sanglas Ariadna , Palomares Francisco 
Published: July 2022

AbstractMammalian carnivores with generalist feeding behaviour should trace and exploit new and predictable food resources quicker and more easily than more specialised species. On the other hand, if the new food resource is spatially and temporally predictable, interference or exploitation competition should arise among members of the carnivore guild, with the expectation that smaller species will not use the food resource or will change their foraging behaviour to avoid conflict with larger species. Here, we studied the response to a new food resource of a mammalian mesocarnivore community in south-western Iberian Peninsula. We installed artificial feeding points supplied with a novel food source and tracked them by camera trapping to investigate whether (1) the new artificial food resource was visited, recognised and exploited by the mesocarnivore guild species; (2) how frequently they used the food; and (3) in case of co-occurrence, if dominant species excluded or reduced the feeding options of subordinates. All target species except the badger recognised and exploited the novel food. More generalist species trended to visit feeding points more frequently and spent more time feeding than less generalist species, even though significant differences were not achieved in all cases. When co-occurring at the same feeding point, the arrival of larger species reduced either the visitation rates, feeding probability or time spent feeding of smaller species. Moreover, some smaller species showed a shift in their normal activity pattern at the feeding points when a larger competitor started to use the food source. Overall, we conclude that active avoidance combined with temporal segregation may help reduce agonistic interactions among competitors for shared resources.
Sex as a Moderator Between Parent Ratings of Executive Dysfunction and Social Difficulties in Children and Adolescents with Autism Spectrum Disorder
Authors: Torske Tonje , Nærland Terje , Quintana Daniel S. , Hypher Ruth Elizabeth , Kaale Anett , Høyland Anne Lise , Hope Sigrun , Johannessen Jarle , Øie Merete G. , Andreassen Ole A. 
Published: July 2022

AbstractGirls and boys might differ in autistic symptoms and associated cognitive difficulties such as executive function (EF). We investigated sex differences in the relationship between parent rated EF and autistic symptoms in 116 children and adolescents (25 girls) aged 5–19 years with an intelligence quotient above 70 and an autism spectrum disorder (ASD) diagnosis. They were rated with the behavior rating inventory of executive function (BRIEF) and the autism diagnostic interview revised (ADI-R). We found a positive association between EF and the ADI-R domains of reciprocal social interaction (p < 0.001) and communication (p = 0.001) in girls, while these relationships were small and non-significant in boys. Our results provide a greater understanding of the sex-specific characteristics of children and adolescents with ASD.
Agile methods in the German banking sector: some evidence on expectations, experiences and success factors
Authors: Brühl Volker 
Published: July 2022

AbstractThe importance of agile methods has increased in recent years, not only to manage IT projects but also to establish flexible and adaptive organisational structures, which are essential to deal with disruptive changes and build successful digital business strategies. This paper takes an industry-specific perspective by analysing the dissemination, objectives and relative popularity of agile frameworks in the German banking sector. The data provides insights into expectations and experiences associated with agile methods and indicates possible implementation hurdles and success factors. Our research provides the first comprehensive analysis of agile methods in the German banking sector. The comparison with a selected number of fintechs has revealed some differences between banks and fintechs. We found that almost all banks and fintechs apply agile methods in IT projects. However, fintechs have relatively more experience with agile methods than banks and use them more intensively. Scrum is the most relevant framework used in practice. Scaled agile frameworks are so far negligible in the German banking sector. Acceleration of projects is apparently the most important objective of deploying agile methods. In addition, agile methods can contribute to cost savings and lead to improved quality and innovation performance, though for banks it is evidently more challenging to reach their respective targets than for fintechs. Overall our findings suggest that German banks are still in a maturing process of becoming more agile and that there is room for an accelerated adoption of agile methods in general and scaled agile frameworks in particular.