bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dextran Sulfate Inhibits Angiogenesis and Invasion of Gastric Cancer by
Interfering with M2-type Macrophages Polarization
Authors: Guo Jiaxin , Xu Yuanyi , Huang Yunning , Li Zhaojun , Ma Qian , Li Mengqi , Zhao Yuan , Li Bing , Tao Yuejia 
Published: July 2022

Purpose:
To explore the effect of dextran sulfate (DS) on the angiogenesis, invasion, and
migration of gastric cancer cells by interfering with the polarization of M2-type macrophages.

Methods:
The infiltration of M2-type macrophages and microvascular density in gastric cancer and
paracancerous tissues were analyzed using immunohistochemistry and immunofluorescence. The effects
of DS on M2-type macrophages and the angiogenesis in metastatic tumors were investigated in
the nude mice intraperitoneal metastasis model using immunohistochemistry and western blot. The differentiation
and polarization of macrophages, immunocytochemistry, western blot, ELISA, and
transwell migration assay were used to evaluate the effect of DS on the polarization of macrophages,
immunocytochemistry, western blot, ELISA, and transwell migration assay were used to evaluate the
effect of DS on the polarization and recruitment capacity of macrophages. Immunocytofluorescence,
tube formation assay, transwell invasion assay, wound healing assay, and western blot were used to
investigate the effect of DS on the angiogenesis, invasion, and migration-promoting phenotype of M2-
type macrophage in a co-culture system of macrophages and gastric cancer cells.

Results:
The infiltration of M2-type macrophages and the microvascular density were highly expressed
and positively correlated in the human gastric cancer tissue. DS can significantly inhibit the intraperitoneal
metastases of gastric cancer in nude mice, and reduce the infiltration of M2-type macrophages
and the angiogenesis in intraperitoneal metastatic tumors. Moreover, DS can prevent the polarization of
M0-type macrophages to M2 type, reduce the expression of M2-type macrophage markers (CD206,
CD163, IL-10, and Arg-1), down-regulate the IL-6-STAT3 pathway, and inhibit the recruitment capability
of M2-type macrophages. Finally, the co-culture experiment showed that DS significantly reduced
the enhancing effects of M2-type macrophages on the angiogenesis, invasion, and migration of
gastric cancer cells, as well as down-regulated the related expressions of proteins (VEGF, N-cadherin,
MMP-2 and Vimentin) in gastric cancer cells.

Conclusion:
DS can reduce the infiltration of M2-type macrophages and the microvascular density in
intraperitoneal metastases of gastric cancer in nude mice, and inhibit the angiogenesis, invasion, and
migration of gastric cancer cells by interfering with the polarization of M2-type macrophages through
repression of the IL-6/STAT3 signaling pathway.
Traditional and Novel Computer-Aided Drug Design (CADD) Approaches in the Anticancer Drug Discovery Process
Authors: Campos Maira Rubi Segura , del Carmen Quintal Bojórquez Nidia 
Published: July 2022

Background:In the last decade, cancer has been a leading cause of death worldwide. Despite the impressive progress in cancer therapy, firsthand treatments are not selective to cancer cells and cause serious toxicity. Thus, the design and development of selective and innovative small molecule drugs is of great interest, particularly through in silico tools.Objective:The aim of this review is to analyze different subsections of computer-aided drug design [CADD] in the process of discovering anticancer drugs.Methods:Articles from the 2008-2021 timeframe were analyzed and based on the relevance of the information and the JCR of its journal of precedence, were selected to be included in this review.Results:The information collected in this study highlights the main traditional and novel CADD approaches used in anticancer drug discovery, its sub-segments, and some applied examples. Throughout this review, the potential use of CADD in drug research and discovery, particularly in the field of oncology, is evident due to the many advantages it presents.Conclusions:CADD approaches play a significant role in the drug development process since they allow a better administration of resources with successful results and a promising future market and clinical wise.
Genetic Analysis of HIV-1 vpr Sequences from HIV-Infected Older
Patients on Long-Term Antiretroviral Therapy
Authors: Ahmad Nafees , Love Maria , Samora Luiza , Barker Danae , Zukosky Priya , Kummet Nathan , Ahmad Aasim , Bernhardt Dana , Tripathi Meghna , Klotz Stephen 
Published: July 2022

Background:
Many HIV-infected individuals have achieved undetectable viral load and
increased CD4 T cell counts due to the success of antiretroviral therapy (ART). However, HIV persists
in resting T cells, monocytes/macrophages and other quiescent cells. Furthermore, the HIV-1
vpr accessory gene may play an important role in the persistence of HIV in these infected patients.

Objectives:
Therefore, we characterized the HIV-1 vpr gene from PBMC DNA of 14 HIV-infected
older patients on long-term ART with mostly undetectable viral load and increased CD4 T cell
counts.

Methods:
Peripheral blood mononuclear cells (PBMC) were isolated from 14 HIV-infected individuals,
followed by extraction of genomic DNA, amplification of HIV-1 vpr gene by polymerase
chain reaction (PCR), cloning of vpr gene in TOPO vector and characterization of correct size
recombinant inserts containing vpr genes. An average of 13 clones were sequenced from each patient,
followed by sequence analysis by bioinformatic tools.

Results:
Phylogenetic analysis of 182 vpr sequences demonstrated that the vpr sequences of each
patient were well separated and discriminated from other patients’ sequences and formed distinct
clusters. The vpr sequences showed a low degree of viral heterogeneity, lower estimates of genetic
diversity and about half of the patients’ sequences were under positive selection pressure. While the
majority of the Vpr deduced amino acid sequences from most patients contained intact open reading
frames, several sequences, mostly from two patients, had stop codons. Numerous patient-specific
and common amino acid motifs were found in deduced Vpr sequences. The functional domains required
for Vpr activity, including virion incorporation, nuclear import of pre-integration complex
and cell cycle arrest, were generally conserved in most Vpr sequences. Several of the known cytotoxic
T-lymphocytes (CTL) epitopes in Vpr showed variation in our patients’ sequences.

Conclusion:
In summary, a low degree of genetic variability, conservation of functional domains
and variations in CTL epitopes were the features of vpr sequences from the 14 HIV-infected older
patients with controlled viremia on long-term ART.
Cognitive Behavior Therapy untuk mengurangi perilaku melukai diri pada kasus depresi
Authors: Esti Paramitayani  
Published: July 2022

Depression is a mood disorder characterized by the absence of hope, prolonged sadness, excessive feelings of helplessness, inability to make decisions, inability to concentrate, loss of enthusiasm for life, feelings of tension and a desire to end life or hurt oneself. The purpose of this study was to see whether self-injury behavior in depression could be reduced after giving Cognitive Behavioral Therapy. The subject is twenty-three years old woman; suffer from depression and often self-harm. The assessments used were clinical interviews, observation, Beck Depression Inventory, graphic tests, Wechsler Adult Intelligence Scale, Thematic Apperception Test, and WHODAS. Frequency self -injury behavior subject was three times a week average. The intervention given is Cognitive Behavioral Therapy to reduce the frequency of self-injury by 10 sessions. The result shows that Cognitive Behavioral Therapy is effective in reducing self-injury behavior and can reduce negative thinking in people with depression.
Somos um milhão de mulheres
Authors: Lopes Natânia , Lopes Giovana 
Published: July 2022

O ensaio Somos Um Milhão de Mulheres é um fragmento de um projeto fotográfico maior, intitulado O Que Você Não Vê,a prostituição vista por nós mesmas, que contou com 16 fotógrafas, trabalhadoras sexuais de vários locais e nichos do mercado da prostituição na cidade do Rio de Janeiro. Foi um projeto artístico e documental desenvolvido no âmbito da pesquisa "Os Impactos dos Mega Eventos Esportivos nos Mercados do Sexo no Rio de Janeiro", que contou com financiamento da FAPERJ durante os anos das Olímpiadas e Para-Olimpíadas na cidade. Através das narrativas das trabalhadoras sexuais e em diálogo e colaboração do movimento brasileiro de prostitutas, procurou-se documentar a relação entre o Estado e seus projetos higienistas orientados por pânicos morais ligados à atividade prostitucional e as formas criativas pelas quais os atores ocupam a cidade, reiventam os espaços, se deslocam e criam, efetivamente, lugar. O ensaio ora apresentado investe numa das possibilidades abertas pelo projeto fotográfico maior do qual faz parte e em diálogo com a pesquisa da qual ele é fruto, oculta as identidades individuais das fotógrafas, indexadas a cada uma das imagens, para fazer emergir uma autoria coletiva de um conjunto de fotografias que é, ele mesmo e em conjunto, um acontecimento.
Three-dimensional assessment of palatal area changes after posterior crossbite correction with tooth-borne and tooth bone–borne rapid maxillary expansion: A randomized controlled trial with 5-year follow-up
Authors: Malmvind Damir , Golež Aljaž , Magnuson Anders , Ovsenik Maja , Bazargani Farhan 
Published: July 2022

ABSTRACT

Objectives
To assess and compare the three-dimensional treatment changes in palatal surface area and volume using either tooth-borne (TB) or tooth bone–borne (TBB) rapid maxillary expanders and to evaluate the long-term effects of the two devices and the incidence of the relapse between the groups.

Materials and Methods
A total of 52 consecutive patients who met the eligibility criteria were recruited and allocated to either the TB group, mean age 9.3 years (standard deviation [SD], 1.3), or the TBB group, mean age 9.5 years (SD, 1.2). Study casts were taken before, directly after, 1 year after, and 5 years after expansion. Study casts were digitized, superimposed, and evaluated. Participants were randomly allocated in blocks of different sizes using the concealed allocation principle in a 1:1 ratio.

Results
Changes in palatal volume, palatal surface area, and palatal projection area within and between the groups up to 5 years after expansion followed the same pattern and did not show any statistically significant differences between the groups. Relapse was seen in 15% of the patients. It seemed that open-bite and a Class III growth pattern could be assumed as prognosis-deteriorating factors in regard to stability of the treatment.

Conclusions
There were no significant differences between the TB and TBB groups in palatal volume, palatal shell area, or palatal projection area directly after expansion or at 1 year and 5 years after expansion, which implies that the two devices gave rise to the same immediate and long-term outcomes.
Barnehagelærarstudentars refleksjonar over barns fleirsanselege utforsking i naturfaglege samtaler
Authors: Fagerbakke Inga Margrethe 
Published: July 2022

Artikkelen presenterer ein casestudie av tre studentar sine transkriberte samtaler om naturfaglege emne med barnehagebarn i alderen 2,5–4 år, og deira refleksjonar rundt desse. Formålet med artikkelen er å belysa korleis studentane stiller seg til barnas fleirsanselege utforsking som ein del av samtala. Funna viser at studentane, gjennom videoanalyse av seg sjølve, blir meir merksame på korleis dei kunne ha fylgt opp fleire av sanseinntrykka til barna og fått dei inn i samtalene. Studentane trekkjer fram manglande bruk av tid, at dei skiftar fokus og at dei stiller feil spørsmål, som forklaringar på kvifor dei ikkje lukkast. I dei tilfella der dei har lukkast, peikar dei på det å la barnet sansa som ein suksessfaktor. Funna viser at produktive spørsmål som handlar om å observera nøye, er ein måte å gripa fatt i barns fleirsanselege utforsking på, men at det ofte blir brukt hypotesedannande spørsmål i samtalene med barna, utan at dette bidrar til meir utforsking i samtalene.
English abstract
Pre-service kindergarten teachers’ reflections over children’s multisensory exploration in scientific dialogues
The article presents a case study of three students’ transcribed conversations with and reflections on scientific conversations with kindergarten children between 2.5–4 years. The purpose of the article is to explain how the students position themselves to the children's multisensory exploration as part of the conversation. The findings show that the students, through video analysis of themselves, became more aware of how they could have followed up more of the children’s sensory impressions, and brought it into the conversations. Students point to their lack of use of time, shift of focus, and asking the wrong questions, as explanations for why they are failing. In those cases where they have succeeded, it is pointed out to let the child sense, as a success factor. The findings show that productive questions that are about observing carefully are a way of tackling children’s multisensory exploration, but that hypothesis-forming questions are often used with the children, without this contributing to more exploration in the conversation.
Computer Vision for Identifying and Classifying Green Coffee Beans: A Review
Authors: Ligar Bonang Waspadadi 
Published: July 2022

Coffee is widely consumed around the world, also considered one of the most important beverages today. Factors contributing to the quality of coffee beans such as color, texture, size, aroma, etc. and other processes along the production chain such as plant, roasting, and grinding. Those processes will be worthless if the quality of the coffee bean is low. It is important to only use the best quality coffee beans. Therefore, the challenge is to develop a system that uses computer vision to either identify high quality beans or classify them by their species to ease the effort needed by all actors in the supply chain. Providing information for end customers is a defining factor to push forward the coffee industry. This paper aims to review literatures within the topic of using computer vision for coffee beans. After reviewing a selected number of studies which corresponds with the topic chosen in our paper, computer vision techniques were used for two main reasons, identification and classification. Researches on this topic are still limited. Hence, it can be concluded that there are still plenty of room for study on this topic. This study also aims to help provide research material for future researchers.
UJI PENINGKATAN KONSENTRASI Metarhizium anisopliae (Metsch.) PADA MEDIA KOMPOS DALAM MENGENDALIKAN LARVA Oryctes rhinoceros L.
Authors: Fauzana Hafiz , Fadilla Miftahul 
Published: July 2022

The Rhinoceros beetle (Oryctes rhinoceros) is the main pest of palm oil (Elaeis guineensis Jacq.). Biological control using Metarhizium anisopliae fungus was effective in controlling O. rhinoceros. M. anisopliae fungus can be applied on palm oil empty bunches (POEB) which composted (Metankos). The research result in 2019 that a concentration of 50 g.l-1 M. anisopliae only kill 56% of O. rhinoceros larvae, so an increase in application concentration was tested. This research aimed to obtain the best concentration by increasing the concentration of M. anisopliae in POEB compost mixed with sawdust in controlling O. rhinoceros L. larvae. The research was conducted at The Plant Pest Laboratory Faculty of Agriculture University of Riau, to January until April 2021, using a completely randomized design (CRD), consist five treatment and four replications, concentration of M. anisopliae in compost was 55 g.l-1 water, 65 g.l-1 water, 75 g.l-1 water, 85 g.l-1 water, 95 g.l-1 water. The research showed that an increase in the concentration of M. anisopliae in compost of 75 g.l-1 water could be categorized as biopesticides because it caused an total mortality is 72,50 ± 5,00. The lethal concentrations 50% and 95% of M. ansiopliae in compost is 48 g.l-1 water approach to concentration 55 g.l-1 water and 140 g.l-1 water approach to concentration 95 g.l-1 water.
PENGARUH METODE DAN DOSIS APLIKASI VERMIKOMPOS PADA BUDIDAYA TANAMAN KAILAN (Brassica oleraceae L.) SECARA HIDROGANIK
Authors: Wisang Qonaiza Gilang , Sholihah Anis , Nurhidayati Nurhidayati 
Published: July 2022

Dengan semakin tingginya tingkat kesadaran masyarakat tentang produk pangan sehat, mendorong berkembangnya budidaya tanaman sayuran secara hidroponik kultur substrat khususnya di wilayah perkotaan. Penggunaan pupuk organik pada sistem budidaya hidroponik masih jarang digunakan. Penelitian ini merupakan percobaan pot di rumah plastik yang menggunakan kultur substrat berupa campuran cocopeat, biochar sekam dan pasir dengan sumber nutrisi berasal dari pupuk vermikompos. Tujuan penelitian ini adalah untuk menjelaskan pengaruh metode aplikasi dan dosis vermikompos padat maupun vermikompos cair terhadap pertumbuhan, dan hasil tanaman kailan. Percobaan ini menggunakan Rancangan Acak Kelompok Faktorial dengan kontrol. Faktor I adalah cara aplikasi vermikompos terdiri dari tiga taraf yaitu M1= Vermikompos padat, M2= kombinasi vermikompos padat dan cair, M3 = Vermikompos cair. Faktor II adalah Dosis Vermikompos yang terdiri dari lima taraf yaitu V1= 100 gram, V2= 200 gram, V3= 300 gram, V4= 400 gram, dan V5= 500 gram per pot. Hasil penelitian menunjukkan bahwa aplikasi vermikompos padat pada dosis 300-500 g/pot memberikan rata-rata pertumbuhan tinggi tanaman (10,25 cm), jumlah daun (7,80) dan luas daun tanaman (295,73 cm2) yang nyata (P<0,05) lebih tinggi dibandingkan dengan perlakuan lainnya. Berat segar total biomassa dan berat segar hasil yang bernilai ekonomis tertinggi terdapat pada perlakuan vermikompos padat pada dosis 500 g/pot masing-masing sebesar 62,14 gram dan 58,33 g, tetapi tidak berbeda nyata dengan perlakuan control yaitu sebesar 66,54 gram dan 61,84 gram. Hasil penelitian ini menyarankan bahwa untk mendapatkan hasil kailan yang tinggi, penggunaan vermikompos padat dengan dosis 500 g/pot direkomendasikan pada budidaya tanaman kailan secara hidroganik.
Identifikasi Human Error Pada Proyek Konstruksi Menggunakan Metode SHERPA dan HEART Serta Perancangan Layout Lokasi Signsystem Visual Berdasarkan Bs-ISO 3864:2011
Authors: Ridwan Gucci Dinda Okta Dwiyanti 
Published: July 2022

Human error merupakan terjadinya kesalahan atau kegagalan dalam menyelesaikan suatu tugas pekerjaan sehingga menimbulkan gangguan terhadap jadwal operasi atau mengakibatkan kerusakan benda dan peralatan. Penelitian ini menggunakan dua metode identifikasi human error meliputi SHERPA dan HEART. kemudian, kajian ini melakukan perancangan layout sign system visual yang akan diletakan di lokasi human error terbesar. Perancangan layout ini menggunakan standar safety sign yaitu BS-ISO 3864:2011. BS-ISO 3864:2011 menetapkan standar internasional untuk tanda dan tanda keselamatan di tempat kerja dan fasilitas umum. Human error yang terjadi adalah operator memiliki alur kerja yang tidak searah sehingga alur kerja operator bolak-balik/berputar-putar, operator tidak memperhatikan bentuk batu yang diambil sehingga ukuran batu menjadi berbeda-beda, melakukan pemeriksaan terhadap ketebalan rumput yang diambil, jika tebal maka tanah bagian bawah rumput dapat di bersihkan terlebih dahulu. Implikasi pada kajian ini Apabila manusia dapat menjaga keselamatannya maka bahaya sebenarnya dapat di hindari oleh manusia dengan cara yang lebih waspada terhadap apapun yang ada di sekitar mereka sebelum bahaya itu terjadi. Human error merupakan terjadinya kesalahan atau kegagalan dalam menyelesaikan suatu tugas pekerjaan sehingga menimbulkan gangguan terhadap jadwal operasi atau mengakibatkan kerusakan benda dan peralatan. Penelitian ini menggunakan dua metode identifikasi human error meliputi SHERPA dan HEART. kemudian, kajian ini melakukan perancangan layout sign system visual yang akan diletakan di lokasi human error terbesar. Perancangan layout ini menggunakan standar safety sign yaitu BS-ISO 3864:2011. BS-ISO 3864:2011 menetapkan standar internasional untuk tanda dan tanda keselamatan di tempat kerja dan fasilitas umum. Human error yang terjadi adalah operator memiliki alur kerja yang tidak searah sehingga alur kerja operator bolak-balik/berputar-putar, operator tidak memperhatikan bentuk batu yang diambil sehingga ukuran batu menjadi berbeda-beda, melakukan pemeriksaan terhadap ketebalan rumput yang diambil, jika tebal maka tanah bagian bawah rumput dapat di bersihkan terlebih dahulu. Implikasi pada kajian ini Apabila manusia dapat menjaga keselamatannya maka bahaya sebenarnya dapat di hindari oleh manusia dengan cara yang lebih waspada terhadap apapun yang ada di sekitar mereka sebelum bahaya itu terjadi.
Analisis Kemampuan Abstraksi Matematis Siswa Sekolah Menengah Pertama Ditinjau Dari Gaya Belajar Tipe Kolb
Authors: Edimuslim Edimuslim 
Published: July 2022

Penelitian ini bertujuan untuk mendeskripsikan level kemampuan abstraksi matematis pada materi geometri (kubus dan balok) ditinjau dari gaya belajar tipe Kolb yang dimiliki siswa, menelaah keterkaitan antara level kemampuan abstraksi matematis dengan gaya belajar tipe Kolb dan mengetahui kesalahan yang dilakukan siswa dalam menyelesaikan soal tes kemampuan abstraksi matematis berdasarkan kriteria Newman Error. Penelitian ini merupakan penelitian deskriptif dengan pendekatan kualitatif. Subjek penelitian ini diperoleh dengan melaksanakan tes gaya belajar menggunakan instrumen KLSI (Kolb Learning Style Inventory) dan soal tes kemampuan abstraksi matematis yang disusun berdasarkan level dan indicator abstraksi matematis. Hasil penelitian ini menunjukkan bahwa 1). Persentase keberadaan tipe gaya belajar Assimilator, Diverger, Accomodator dan Converger berturut-turut adalah 53,3%, 20%, 13,3% dan 13,3%. 2). Siswa dengan gaya belajar assimilator, diverger dan accommodator lebih banyak berada pada level 1 dan level 2 dengan kriteria transisi. Sedangkan siswa dengan gaya belajar converger mampu mencapai level 1, level 2 dan level 3 dengan kriteria lengkap. 3). terdapat keterkaitan antara level abstraksi matematis dengan gaya belajar tipe converger. 4). Jenis kesalahan yang ditemui Ketika siswa menyelesaikan soal abstraksi matematis diantaranya kategori Encoding error, transformation error dan process skill error.
Makna Syukur Dalam Al-Qur’an Pada Tradisi Babarit Di Kuningan
Authors: Hidayat Hamdan 
Published: July 2022

AbstractThis paper discusses the meaning of gratitude in the Qur'an in the Babarit Tradition in Kuningan. This study uses the history-social approach method, the results of the study show that applying a form of gratitude to the Babarit tradition in Kuningan, there is a uniqueness, namely Babarit is an implementation of gratitude which is packaged in the routine activities of the Sundanese community which then has cultural values as a Sundanese ethnic identity and also as a selling point for tourism attraction. in Kuningan. AbstrakTulisan ini membahas tentang makna syukur dalam al-Qur’an pada tradisi kabarit di Kuningan. Penelitian ini menggunakan metode pendekatan history-social. hasil dari penelitian menunjukkan bahwa mengaplikasikan bentuk rasa syukur pada tradisi Babarit di Kuningan terdapat sebuah keunikan, yaitu Babarit yang merupakan implementasi dari rasa syukur yang dikemas dalam kegiatan rutin masyarakat Sunda yang kemudian terdapat nilai budaya sebagai identitas suku Sunda dan juga sebagai nilai jual daya tarik pariwisata di Kuningan. Kata Kunci: Babarit, Syukur, Kuningan.