bluuebunny/crossref_metadata_2025_split huggingface dataset:
c-Myc-activated miR-3682-3p aggravates the metastasis and stemness in hepatocellular carcinoma cells by regulating PTEN/PI3K/AKT signaling
Authors: Zhang Yu , Cai Hua , Wu Ming-Hao , Zhu Dan-Hua , Wang Xiang-Yang , Chen Zhi-Yuan , Yang Li , Liu Peng , Liu Zhan 
Published: July 2022

Abstract
Objective: To elusive the underlying mechanism and functions of miR-3682-3p in hepatocellular carcinoma (HCC).Methods: Thirty pairs of tumor tissues and adjacent tissues were obtained from HCC patients. mRNA and protein expressions were detected by quantitative real-time PCR and western blot, respectively. The migration and invasion were measured using transwell or wound healing assays. Dual luciferase and ChIP assays were utilized to detect gene interactions.Results: miR-3682-3p was highly expressed in HCC tissues and cell lines. Silencing of miR-3682-3p prevented cell migration and invasion, and induced the enhancement of E-cadherin expression and the decreased levels of N-cadherin, Vimentin and Snail as well as expressions of SOX2, OCT4 and Bmi1, restraining metastasis and stemness of HCC in vitro. Mechanistically, miR-3682-3p was positively activated by c-Myc, and could directly target PTEN to activate PI3K/AKT pathway. In addition, inhibition of PTEN weakened the anti-metastatic and anti-stemness effects of miR-3682-3p downregulation in HCC cells. Conclusion: From these findings, we concluded that miR-3682-3p promoted HCC metastasis and stemness through PTEN/PI3K/AKT signaling, implying that miR-3682-3p might be a promising target for HCC clinical treatment.
Enhanced Expression of RAGE AXIS is Associated with Severity of COVID-19 in Patients with Comorbidities
Authors: Khan Gulnaz , Sohail Fadieleh A. , Khan Bushra , Rafi Sidra , Nasir Shumail , Waraich Rizwana Sanaullah 
Published: July 2022

Abstract
There is a limited understanding of molecular and cellular events that derive disease progression in patients with COVID-19. Receptor for Advanced Glycation End Products (RAGE) is hyperactive in development and complications of several diseases by mediating oxidative stress and inflammation in the body. The present study aims to explore activation of RAGE signaling in patients infected with SARS-CoV-2 with preexisting comorbidities. Enhanced levels of ligands of RAGE including AGEs, S100, and HMGB-1 were observed in Covid 19 patients with severe diseases, however, their level was significantly higher in COVID-19 patients with comorbidties as compared to COVID-19 patients without comorbidties. The Expression of RAGE in parallel to ligands accumulation, was significantly increased in patients with severe disease and comorbidities as compared to COVID-19 patients with sever disease without comorbidities. The expression of downstream effectors of RAGE including STAT-3 and NF-kB were also enahnced and their activity was increaed in COVID-19 patients with comorbisdities. Levels of inflammatory and oxidative stress biomarkers were markeldy in COVID-19 patients with comorbidties as compared to COVID-19 patients without comorbidties. We conclude that upregulated RAGE axis is favorable to worsen the severity of the SARS-CoV-2 infection in patients with preexisting comorbidities and partly explain inflammatory and oxidative stress strom in severe COVID-19 patietnts.
Renal insufficiency and magnesium deficiency hinder the formation of biologically active cholecalciferol
Authors: Rothen Jean-Pierre , Rutishauser Jonas , Arnet Isabelle , Allemann Samuel 
Published: July 2022

Abstract
Background: Vitamin D is synthesized in the skin or supplied. Cholecalciferol is hydroxylated in the liver to 25(OH) vitamin D [25D]. 25D is further hydroxylated in the kidney to 1,25(OH) vitamin D [1,25D]. Catabolism occurs by further hydroxylation. Magnesium is a cofactor of all involved hydroxylases. Aim: To investigate the association between serum levels of 1,25D and serum magnesium concentrations, and renal function.Method: Laboratory values measured in an outpatient cohort over 2 years were analysed. Results: Renal function and magnesium level did not influence 25D values. Mean serum 1,25D values decreased from 106.5 ± 44.3 pmol/l in individuals with normal renal function to 51.7 ± 18.9 pmol/l in those with severe renal insufficiency (p<0.01). A weak positive correlation was observed between 1,25D and eGFR (r=0.317), and between 1,25D and serum magnesium (r=0.217). Conclusion: Impaired renal function and low magnesium serum levels are slightly associated with low 1,25D concentrations. Measuring 25D, but not 1,25D, may overestimate the patient’s vitamin D status. In patients with renal insufficiency adequate magnesium supply should be ensured.
Impact of HLA Class I Antigen, Killer Ig-like Receptor and FCGR3A Genotypes in Breast Cancer Susceptibility and Tumor stage
Authors: Canossi Angelica , Aureli Anna , Beato Tiziana Del , Novelli Giorgio , Buonomo Oreste , Rossi Piero , Venditti Adriano , Papola Franco , Sconocchia Giuseppe 
Published: July 2022

Abstract
The identification of clinical pathological role of genes regulating NK cell function, including HLA, KIR and FCGR3A, in breast cancer (BC) may be a challenge. The objective of this study was to determine whether these genes have an impact on the BC susceptibility and progression. In this study, 47 BC Italian patientswere genotyped by PCR-SSP typing (KIR) and PCR-SBT method (HLA-C and FCGR3A). HLA-C allele analysis showed that the HLA-C*07:02:01 (p = 0.04, OR = 4.867) and HLA-C*04:01:01 alleles (p = 0.04 OR = 2.364) were predisposing to tumor progression, and instead the HLA-C*05:01:01 allele was protective against disease (p = 0.019 OR = 0.087). In addition, we highlighted a significant reduction of the KIR2DS4ins in BC patients (p = 0.022) and an increased coexistence of KIR2DL1 and KIR2DS1 genes in advanced BC patients compared to early stages (p = 0.002). The concurrent lack of KIR2DL2 and KIR2DS4 genes in the presence of HLA-C2 alleles was significantly associated with increased susceptibility to BC (p = 0.012 OR = 5.020) or with lymph node involvement (p = 0.008 OR = 6.375). We identified different combinations of the FCGR3A-48/158 haplotypes and KIR genes in BC patients compared to controls. Our study provides evidence that the combined analysis of these biomarkers might help predict genetic risk scores for tailored screening of BC patients.
Bone Scintigraphy Outperformed Anatomic Images in Frostbite Injuries, a Case Report
Authors: RONCHI BRUNELA , Peña Gustavo Agustin , Albertti Carla 
Published: July 2022

Abstract
Frostbite is a localized cold thermal injury, as a result of tissue exposure to temperatures below freezing point for a prolonged period of time .The spectrum of injury is broad, thus frostbite injuries may have deleterious effects with the possibility of losing part or whole extremities. We aim to present the case of a 38 years-old male patient evaluated with multiphase technetium-99m-methylene diphosphonate bone scintigraphy. This methodology may accelerate clinical management of frostbite injuries because it provides precise clinical-imaging correlation by determining the extent of injury and can accurately predict the level of amputation if required.
Low bit error rate quantum key distribution based on integrated silicon photonics
Authors: Zhen Zhen , Jin Si-Yue , Jie Ren , Xu Xing-Sheng 
Published: July 2022

Abstract
Quantum communication has extremely high security and is the development direction of future communication networks. In this paper, an on-chip path, off-chip polarization encoded quantum key distribution chip is designed and fabricated using advanced silicon photonics fabrication technology. Using a special two-dimensional grating, we can convert light of different path states into corresponding polarization states and propagate in free space. The performance of the basic optics, waveguides, gratings, and Mach-Zehnder interferometers on the chip is tested. In the demonstrated quantum key distribution experiment, the quantum bit error rate is as low as 1.08%. It lays the foundation for the future development of advanced integrated quantum information.
Reoperation Frequency after Transverse Preputial Island Flap Urethroplasty in Treating of Severe Hypospadias: A Single Center Study
Authors: Li Jiayi , Liu Pei , Yang Zhenzhen , Wang Xinyu , Fan Songqiao , Li Zonghan , Song Hongcheng , Zhang Weiping 
Published: July 2022

Abstract
Background: Considering the continuing debate on the best technique to treat serve hypospadias, the reoperation frequency after Transverse Preputial Island Flap Urethroplasty (TPIFU) is lacking. In the present study, we reported our clinical outcomes of severe hypospadias treated with one-staged TPIFU and analyzed the operation frequency. Methods: We retrospectively analyzed the clinical data of severe hypospadias patients who underwent one-stage TPIFU from December 2018 to December 2019. A stepwise approach was used to manage the curvature. Severe hypospadias was defined as the residual curvature was > 30° after degloving. Urethroplasty complications included fistula, urethral stricture, and diverticulum. The short-term cure was identified as no complications occurring for 12 months after the date of last-time surgery. The reoperation rate and operation frequency of TPIFU were analyzed. Results: A total of 136 patients who underwent one-stage TPIFU were included in the study. The follow-up after primary urethroplasty ranged from 22 to 50 months. The median age at primary surgery was 22.5 months (range from 13 to 132 months). After primary TPIFU surgery, 53(39%) patients accepted reoperation handling with complications. Among them, 24 patients (17.6%) developed fistula, 17 patients (12.5%) developed urethral stricture and 11 patients (8.1%) developed diverticulum. After the second surgery, 5/17 patients remained fistula, 5/17 patients remained urethral stricture, and 7/17 patients remained diverticulum. Overall, 61% (85 patients) met the cured standard after the primary operation, and the two operations cure rate was 87.5% (119 patients). 91.2% (124 patients) were cured in three operations.Conclusions: The present study provides a novel perspective to evaluate the surgery technique through the surgery number to cure the patients, rather than simply comparing the complication rate. Our result demonstrated that the complication rates of primary TPIFU were relatively high, but the cure rate after two or three operations was acceptable.
Performance analysis of a queueing system based on working vacation with repairable fault in the P2P network
Authors: Wang Shunzhi , Ma Zhanyou , Wang Rong , Fang Wenming 
Published: July 2022

Abstract
Nowadays, P2P (Peer-to-Peer) absolutely d-\\ominates the Internet as a traffic in content distribution and sharing service, the issue of energy consumption has emerged as a hot issue. This paper combines the basic theoretical knowledge of queueing theory with the currently more popular hybrid P2P network structure model. In order to address the problem of wasted energy consumption generated by unnecessary online behavior of nodes, the working sleep mechanism for the serving nodes is introduced, and the synchronous multiple working vacation strategy is also adopted to decrease the system energy consumption. In the meantime, negative customer and feedback strategies are introduced. Then a queueing model with working vacation and repairable fault is built, which used to simulate the resource transmission process of P2P and to study the performance of P2P sharing system. The steady-state probability vector is derived by employing quasi-birth-and-death process (QBD) and matrix-geometric solution method. And the expressions for system performance indicators, such as the average delay, are obtained by employing Gauss-Seidel iteration method. Through numerical experiments, the performance indicators of the P2P resource sharing system are studied, which provides a theoretical basis to decrease online energy consumption of nodes in the P2P network system. And through employing Nash equilibrium and social optimal strategy, the value of the social maximum benefit under the social optimization is obtained.
Effect of cervical brace on postoperative axial symptoms in patients with Anterior Cervical Discectomy and Fusion
Authors: Yu Qunfei , Ren Ying , Wang Zhan , Xu Guoping , Ma Yaojing , Zhang Hua 
Published: July 2022

Abstract
Objective This study was intended to investigate the effect of cervical brace on postoperative axial symptoms in patients with single-segment anterior cervical discectomy and fusion (ACDF). Methods Retrospective analysis of patients who underwent anterior cervical single-segment ACDF from May 2020 to August 2021 was performed. Patients were divided into the cervical brace group (Group 1) and the non-cervical brace group (Group 2) according to whether to wear cervical brace in postoperative patients. The incidence of axial symptoms ,cervical mobility and postoperative quality of life were compared between the two groups. Results A total of 121 patients, 62 in the Group 1 and 59 in the Group 2. The general data of age, gender, BMI, smoking status, disease duration has no statistically differences. The incidence of axial symptoms at 1 month after surgery in the Group 2 was significantly lower. The cervical mobility in the Group 2 at 1 month after surgery were significantly higher. Conclusion The absence of brace after surgery in patients with single-segment ACDF can reduce the incidence of early postoperative axial symptoms, improve their quality of life, and facilitate the recovery of postoperative cervical mobility.
Meeting global challenges with coherent policies producing more researchers
Authors: Sanganyado Edmond , Siyawamwaya Margaret , Nkomo Simbarashe , Gwenzi Willis , Macherera Margaret , Banda Annabel , Teta Charles , Nunu Wilfred Njabulo , Murungweni Chrispen , Gandiwa Edson , Mashe Tapfumanei 
Published: July 2022

Abstract
Sustainable Development Goal 9 encourages building resilient infrastructure, fostering innovation, and promoting sustainable infrastructure by significantly increasing the number of researchers. Using Zimbabwe as a typical developing country, we analyzed ten continental, five regional, five national, and 32 sectoral science policies on their commitment to increasing the number of researchers. Our results showed that while the African Union urged member states to increase their number of researchers, the Southern African Development Community and most government departments in Zimbabwe largely ignored this recommendation. Interestingly, the national science, technology, and innovation policies in Zimbabwe have adopted the African Union guidelines. Our results suggest a lack of coordination between government departments in Zimbabwe. We propose a policy framework for increasing the number of researchers that fosters multilevel and multisectoral policy coordination, provides a link between policymakers and researchers, and promotes regional integration.
The immune evasion ability of Delta variant is comparable to that of Beta variant in South Africa
Authors: He Daihai , Chen Boqiang , Zhao Shi , Stone Lewi 
Published: July 2022

Abstract
Background The high immune evasion ability of SARS-COV-2 Omicron variant surprised the world and appears to be far stronger than any previous variant. Previous to Omicron it has been difficult to assess and compare immune evasion ability of different variants, including the Beta and Delta variants, because of the relatively small numbers of reinfections and because of the problems in correctly identifying reinfections in the population. This has led to different claims appearing in the literature. Thus we find claims of both high and low immune evasion for the Beta variant. Some findings have suggested that the Beta variant has a higher immune evasion ability than the Delta variant in South Africa, and others that it has a lower ability. MethodIn this brief report, we re-analyse a unique dataset of variant-specific reinfection data and a simple model to correct for the infection attack rates of different variants.Result We find that a model with the Delta variant to have an equal or higher immune evasion ability than Beta variant is compatible with the data.ConclusionWe conclude that the immune evasion ability of Beta variant is not stronger than Delta variant, and indeed, the immune evasion abilities of both variants are weak in South Africa.
Larvae density, breeding habitat preference and insecticide susceptibility status of Anopheles gambiae (Diptera: Culicidae) in Assosa district, western Ethiopia
Authors: Ejeta Desta , Tefera Tadesse 
Published: July 2022

Abstract
Background
Malaria vectors control interventions mainly rely on the control of adults using indoor residual sprayings and long-lasting insecticidal nets in Ethiopia. However, the emergence and widespread of insecticide resistance in the primary malaria vector, Anopheles gambiae sinso lato challenges the malaria vector control efforts in Ethiopia. The aim of this study was to investigate species composition, abundance and insecticide resistance status of main malaria mosquitoes in Assosa district, Benishangul Gumuz, Western Ethiopia.
Methods
The study was conducted from July 2021 to June 2022. After identification of possible breeding sites, Anopheles mosquito larvae were collected using a standard dipper from varies breeding habitats. Susceptibility tests were conducted on wild population of female An. gambiae complex following World health organization standard susceptibility test procedure. Insecticide impregnated papers with deltamethrin (0.05%), alphacypermethrine (0.05%), propoxur (0.1%), and primiphos methyl (0.25%), were used for the study.
Result
Six major Anopheles mosquitoes’ larvae breeding habitats were identified in four villages, namely, drainage ditch, swamp, pond, animal footprint, sewerage and stagnant water. A total of 2051 Anopheles larvae were collected and identified. Out of all collected Anopheles larvae, 1884 larvae were reared and identified to four species (An. gambiae s.l., An. coustani, An. ziemanni and An. funestus). An. gambiae s.l. were found to be the dominant species (1732, 84.4%) species and the lowest An. funestus were collected (n = 17, 0.9%). The most productive habitats were stagnant water (n = 752; 39. 9%) with larvae/dip while sewerage were detected to be least productive (n = 71; 3.76%) for Anopheles mosquito larvae with mean density of 1.2 and 0.03 larvae/dip, respectively, (P < 0.05). The susceptibility result indicated that the population of An. gambiae s.l in the study area found resistant against alphacypermethrine, deltamethrin and permethrin 63%, 71% and 43% respectively. The result revealed that the vector is susceptible to pirimiphos-methyl and propoxur with 100% susceptibility rate.
Conclusion
Anopheles gambiae complex is the predominant malaria vector in Assosa District. Anopheles gambiae complex developed high level of resistance against most of insecticide classes. Pirmiphosmethyl and propoxur are effective insecticides for malaria vector control in Assosa district, Ethiopia.
Prediction of violence in schizophrenia using sMRI, based on machine learning algorithms
Authors: Yu Tao , Pei Wenzhi , Xu Chunyuan , Zhang Xulai , Deng Chenchen 
Published: July 2022

Abstract
Background: Violent behavior in patients with schizophrenia (SCZ) is a major social problem. The early identification of SCZ patients with violence can facilitate implementation of targeted intervention. Methods: A total of 57 male SCZ patients were recruited into this study. The general linear model was utilized to compare differences in structural magnetic resonance imaging (sMRI) including gray matter volume, cortical surface area, and cortical thickness between 30 SCZ patients who had exhibited violence and 27 SCZ patients without a history of violence. Based on machine learning algorithms, different sMRI features between groups were integrated into the prediction models for identification of SCZ patients with violence. Results: After controlling for the whole brain volume and age, the general linear model showed significant reductions in right bankssts thickness, inferior parietal thickness as well as left frontal pole volume in the patients with SCZ and violence relative to those without violence. Among seven machine learning algorithms, Support Vector Machine (SVM) have better performance in differentiating patients with violence from those without violence, with its balanced accuracy and area under curve (AUC) reaching 0.8231 and 0.841, respectively. Conclusions: Patients with SCZ who had a history of violence displayed reduced cortical thickness and volume in several brain regions. Based on machine learning algorithms, structural MRI features are useful to improve predictive ability of SCZ patients at particular risk of violence.
Characterization and identification of microRNA from Andrographis paniculata and its role in Cancer: A Cross Kingdom Approach
Authors: Motwani Harsha , Gadhavi Harshida , Mangukia Naman , Dixit Nandan , Rawal Rakesh M. , Patel Saumya K. , Solanki Hitesh A. 
Published: July 2022

Abstract
Background and Objective Andrographis paniculata belonging to Acanthaceae family is well-known potential hepatoprotective herb. The role of diet on human health and diseases including Hepatocellular Carcinoma has been demonstrated by innumerable studies which have also testified that plant-derived micro-RNAs, transferred to animals via oral consumption wherein it alters host gene expression and this provides the evidence for inter-species gene regulation. Herein, we aim to explore the role of predicted miRNAs of A. paniculata on human health.Methods and ResultsIn the present study, A. paniculata miRNAs have been predicted and their impact on human transcriptome is uncovered by employing computational approach. By implementing miRNA prediction on A. paniculata genomic data and plant miRNA sequences present in miRBase repository, we have identified 20 miRNAs which are confined to 16 miR families. 197 human target genes were predicted for the identified miRNAs, which were further subjected to Gene Ontology analysis and network analysis revealing their significant role in various biological processes and signaling pathways. The identified hub proteins HGF, NRP2 and CCND1 show their significant involvement in cancerous pathways including STAT3, MAPK, TGF-β, mTOR and VEGFR pathways. Deregulation of these pathways has been reported as oncogenic response involved in Hepatocellular Carcinoma, cell proliferation and metastasis.ConclusionThe findings of our study suggest potentially beneficiary role of A. paniculata miRNAs in regulating cancer via cross-species genetic regulation. Conclusively, our study sustains the promising theory of inter-species gene regulation.
Baicalin blocked colon cancer cell cycle and inhibited cell proliferation through upregulating miR-139-3p by targeting CDK16
Authors: Cai Rong , Zhou Yan-Ping , Li Yun-Hai , Zhang Jin-Jin , Hu Zuo-Wei 
Published: July 2022

Abstract
Background: Baicalin was reported to facilitate the apoptosis of colon cell and inhibit tumor growth in vivo. However, the specific mechanism remains largely unknown. Methods: Relative mRNA level was tested via qRT-PCR. Cell proliferation, viability, and cell cycle phases were evaluated using MTT, colony formation, and flow cytometry assays. Interaction of miR-139-3p/CDK16 was measured via dual-luciferase reporter assay. Results: Baicalin increased miR-139-3p expression, while decreased CDK16 levels, as well as blocked cell cycle and inhibited cell proliferation in colon cancer cells. miR-139-3p silencing or CDK16 overexpression abolished the inhibitory effects of baicalin on colon cancer proliferation. MiR-139-3p direct targeted and interacted with CDK16. The protective functions of miR-139-3p knockdown on tumor cells were abrogated by silencing CDK16.Conclusion: Our findings suggested that baicalin inhibited colon cancer growth through modulating miR-139-3p/CDK16 axis in vitro.