bluuebunny/crossref_metadata_2025_split huggingface dataset:
Augmentation Mammoplasty With External Volume Expander–Assisted Autologous Fat Grafting in 305 Asian Patients
Authors: Gao Qiuni , Liu Chengsheng , Qi Zuoliang , Zhai Peiming , Qi Jun , Yang Zhenyu , Hu Yuling , Yuan Xihang 
Published: February 2022

Abstract

Background
External volume expander (EVE)-assisted autologous fat grafting is suitable for breast augmentation, but no large sample study in Asia has confirmed this method.

Objectives
The authors reported their experience and outcomes in augmentation mammoplasty with EVE-assisted autologous fat grafting.

Methods
A retrospective study was conducted in 305 female patients who underwent augmentation mammoplasty with EVE-assisted fat grafting between September 2012 and December 2020. Doctors utilized Crisalix (Crisalix S.A., Lausanne, Switzerland) for 3-dimensional (3D) imaging acquisition to measure the increase in breast volume to evaluate doctor satisfaction. The Preoperative Satisfaction with Breast and BREAST-Q questionnaires were employed to assess patients’ preoperative and postoperative satisfaction, respectively.

Results
The 305 female patients were aged 18 to 50 years (mean, 35.9 years). Among them, 68.52% were “very satisfied,” 18.69% were “somewhat satisfied,” 11.15% were “somewhat dissatisfied,” and 1.64% were “very dissatisfied” based on BREAST-Q analysis, whereas 100% were dissatisfied according to the Preoperative Satisfaction with Breast questionnaire. Doctors employed Crisalix to measure the increase in breast volume to evaluate doctor satisfaction. The results showed 76.01% had an increase in breast volume of 150 to 250 mL or &gt;250 mL and were “satisfied” and “very satisfied,” respectively, 21.64% had an increase of 50 to 149 mL and were “somewhat satisfied,” and 2.30% had an increase &lt;50 mL and were “dissatisfied.” There were no complications, such as obvious fat liquefaction, infection, or fat embolism.

Conclusions
Augmentation mammoplasty with EVE-assisted fat grafting is effective and satisfying in China. Crisalix for 3D imaging acquisition is convenient and effective in measuring breast volume.

Level of Evidence: 4
TSAFinder: exhaustive tumor-specific antigen detection with RNAseq
Authors: Sharpnack Michael F , Johnson Travis S , Chalkley Robert , Han Zhi , Carbone David , Huang Kun , He Kai 
Published: February 2022

Abstract

Motivation
Tumor-specific antigen (TSA) identification in human cancer predicts response to immunotherapy and provides targets for cancer vaccine and adoptive T-cell therapies with curative potential, and TSAs that are highly expressed at the RNA level are more likely to be presented on major histocompatibility complex (MHC)-I. Direct measurements of the RNA expression of peptides would allow for generalized prediction of TSAs. Human leukocyte antigen (HLA)-I genotypes were predicted with seq2HLA. RNA sequencing (RNAseq) fastq files were translated into all possible peptides of length 8–11, and peptides with high and low expressions in the tumor and control samples, respectively, were tested for their MHC-I binding potential with netMHCpan-4.0.

Results
A novel pipeline for TSA prediction from RNAseq was used to predict all possible unique peptides size 8–11 on previously published murine and human lung and lymphoma tumors and validated on matched tumor and control lung adenocarcinoma (LUAD) samples. We show that neoantigens predicted by exomeSeq are typically poorly expressed at the RNA level, and a fraction is expressed in matched normal samples. TSAs presented in the proteomics data have higher RNA abundance and lower MHC-I binding percentile, and these attributes are used to discover high confidence TSAs within the validation cohort. Finally, a subset of these high confidence TSAs is expressed in a majority of LUAD tumors and represents attractive vaccine targets.

Availability and implementation
The datasets were derived from sources in the public domain as follows: TSAFinder is open-source software written in python and R. It is licensed under CC-BY-NC-SA and can be downloaded at https://github.com/RNAseqTSA.

Supplementary information
Supplementary data are available at Bioinformatics online.
Type-2 cGMP-dependent protein kinase suppresses proliferation and carcinogenesis in the colon epithelium
Authors: Islam Bianca N , Sharman Sarah K , Hou Yali , Wang Rui , Ashby Justin , Li Honglin , Liu Kebin , Vega Kenneth J , Browning Darren D 
Published: February 2022

Abstract
A large body of evidence has demonstrated that cyclic-guanosine monophosphate (cGMP), signaling has anti-tumor effects that might be used for colon cancer prevention. The tumor-suppressive mechanism and the signaling components downstream of cGMP remain largely unknown. The present study has characterized the expression of cGMP-dependent protein kinases (PKG1, PKG2) in normal and cancerous tissue from human colon. PKG1 was detected in both normal and tumor tissue, where it localized exclusively to the lamina propria and stroma (respectively). In contrast, PKG2 localized specifically to the epithelium where its expression decreased markedly in tumors compared to matched normal tissue. Neither PKG isoform was detected at the RNA or protein level in established colon cancer cell lines. To test for a potential tumor-suppressor role of PKG2 in the colon epithelium, Prkg2 knockout (KO) mice were subjected to azoxymethane/dextran sulfate-sodium (AOM/DSS) treatment. PKG2 deficiency was associated with crypt hyperplasia (Ki67) and almost twice the number of polyps per mouse as wild-type (WT) siblings. In vitro culture of mouse colon epithelium as organoids confirmed that PKG2 was the only isoform expressed, and it was detected in both proliferating and differentiating epithelial compartments. Colon organoids derived from Prkg2 KO mice proliferated more rapidly and exhibited a reduced ability to differentiate compared to WT controls. Taken together our results highlight PKG2 as the central target of cGMP in the colon, where it suppresses carcinogenesis by controlling proliferation in an epithelial-cell intrinsic manner.
Performance comparison of mono and polycrystalline silicon solar photovoltaic modules under tropical wet and dry climatic conditions in east-central India
Authors: Baghel Niranjan Singh , Chander Nikhil 
Published: February 2022

Abstract
This work focuses on the performance comparison of monocrystalline and polycrystalline Si solar photovoltaic (SPV) modules under tropical wet and dry climatic conditions in east-central India (21.16° N 81.65° E, Raipur, Chhattisgarh). This study would help to select the SPV module for system installation in the east-central part of the country. For comparative analysis, we used performance ratio (PR) and efficiency as figures of merit. The plane-of-array (POA) irradiance was used to determine the efficiency of the modules. The decomposition and transposition models calculated the POA values from the measured global horizontal irradiance. The data were analysed systematically for 6 months in the non-rainy season, from October 2020 to March 2021. Special attention was given to solar irradiance, ambient temperature and module temperature—the parameters that affect the performance of PV modules. The month of October showed the highest variation in irradiance and temperature. The highest average module temperatures (51–52°C) were observed in October–November, while the lowest average module temperatures (34°C for mono-Si and 36°C for poly-Si) were observed in December. The highest value of average monthly POA irradiance (568 W/m2) was observed in February and the lowest (483 W/m2) in December. The results showed that the monocrystalline SPV module performed better than the polycrystalline module under all weather conditions. The maximum observed values of mono-Si and poly-Si panel PRs were 0.89 and 0.86, respectively, in December. Thermal losses were higher with higher module temperatures in October and November, and lower in December due to lower temperatures. The energy yield was calculated from the measured data and compared with PVSyst simulations.
Transition to cleaner cooking energy in Ghana
Authors: Bofah Richard Osei , Appiah-Konadu Paul , Ngwu Franklin Nnaemeka 
Published: February 2022

Abstract
Progress towards the Sustainable Development Goal 7 and other related goals hinges on increased access to clean energy alternatives for all people irrespective of where they live. However, most developing countries including Ghana still rely largely on traditional biomass as the main source of household energy as a result of a myriad of challenges. From the foregoing, the present study uses the Ghana Living Standard Survey 7 (GLSS 7) household data and the multinomial logit regression model to analyse the factors that determine the transition to cleaner cooking energy in Ghana. The analysis shows that the main determinants of household energy choice in Ghana are education, household dwelling type, household size, employment and income group. Whereas education, modern housing, paid employment and higher income increase the adoption of cleaner energy, a higher dependency ratio and employment in the informal sector increase the likelihood of using unclean energy. Increased access to education and the adoption of policies to improve housing conditions, employment and incomes are recommended to encourage the adoption of cleaner energy alternatives.
Analysis of routes for electrochemical conversion of CO2 to methanol
Authors: Li Pingping , Gong Siqi , Li Chufu , Liu Zhien 
Published: February 2022

Abstract
In the context of peak carbon and carbon neutrality, the utilization of CO2 has attracted attention with the aim of reducing carbon emissions by converting CO2 into high-value chemicals or energy. Methanol (MeOH), which is both a hydrogen and a carbon carrier, is considered the most promising among the CO2-conversion products. This paper focus on routes for electrochemical conversion of CO2 to MeOH using green power and green hydrogen to achieve negative CO2 emissions. Three feasible technical routes for electrochemical conversion of CO2 to MeOH are proposed in this paper: Route 1, electrolysis of water to H2 and hydrogenation of CO2 to MeOH; Route 2, electrochemical reduction of CO2 to MeOH; and Route 3, co-electrolysis of CO2–H2O to syngas and synthesis of MeOH from syngas. Techno-economic assessments of the three routes are conducted using technical maturity surveys, system simulations and cost analyses to provide reference data for route selection for CO2 conversion to MeOH in China. Compared with the other routes, Route 1 is advantageous in terms of technical maturity and commercial application prospects. Although Route 1 is presently economically unviable, it is expected to achieve profitability and commercial application in the future with decreases in the cost of renewable power and continuous development of water-electrolysis technology.
A roadmap to parathyroidectomy for kidney transplant candidates
Authors: Cianciolo Giuseppe , Tondolo Francesco , Barbuto Simona , Angelini Andrea , Ferrara Francesca , Iacovella Francesca , Raimondi Concettina , La Manna Gaetano , Serra Carla , De Molo Chiara , Cavicchi Ottavio , Piccin Ottavio , D'Alessio Pasquale , De Pasquale Loredana , Felisati Giovanni , Ciceri Paola , Galassi Andrea , Cozzolino Mario 
Published: February 2022

ABSTRACTChronic kidney disease mineral and bone disorder may persist after successful kidney transplantation. Persistent hyperparathyroidism has been identified in up to 80% of patients throughout the first year after kidney transplantation. International guidelines lack strict recommendations about the management of persistent hyperparathyroidism. However, it is associated with adverse graft and patient outcomes, including higher fracture risk and an increased risk of all-cause mortality and allograft loss. Secondary hyperparathyroidism may be treated medically (vitamin D, phosphate binders and calcimimetics) or surgically (parathyroidectomy). Guideline recommendations suggest medical therapy first but do not clarify optimal parathyroid hormone targets or indications and timing of parathyroidectomy. There are no clear guidelines or long-term studies about the impact of hyperparathyroidism therapy. Parathyroidectomy is more effective than medical treatment, although it is associated with increased short-term risks. Ideally parathyroidectomy should be performed before kidney transplantation to prevent persistent hyperparathyroidism and improve graft outcomes. We now propose a roadmap for the management of secondary hyperparathyroidism in patients eligible for kidney transplantation that includes the indications and timing (pre- or post-kidney transplantation) of parathyroidectomy, the evaluation of parathyroid gland size and the integration of parathyroid gland size in the decision-making process by a multidisciplinary team of nephrologists, radiologists and surgeons.
Total transfemoral access in a new off-the-shelf thoracoabdominal inner-branched endograft
Authors: Andic Mateja , Mustafi Migdat , Schlensak Christian , Lescan Mario 
Published: February 2022

Abstract
Branched endovascular aneurysm repair (BEVAR) is increasingly being performed with the off-the-shelf devices. We evaluated the use of the wire-assisted sheath stabilization technique for the new inner branch-based (iBEVAR), off-the-shelf E-nside endograft in 2 patients. The cannulation of the 8 reno-visceral target vessels and the BSG implantation were successfully performed using the total transfemoral approach. Complications were not observed during the 3-month follow-up. The wire-assisted sheath stabilization technique was shown feasible in an off-the-shelf iBEVAR.
Feasibility of the SINERGIAPS (“Sinergias entre profesionales y pacientes para una Atención Primaria Segura”) intervention for improving patient safety in primary care
Authors: Serrano-Ripoll Maria J , Fiol-deRoque Maria A , Valderas José M , Zamanillo-Campos Rocío , Llobera Joan , de Labry Lima Antonio Olry , Pastor-Moreno Guadalupe , Ricci-Cabello Ignacio 
Published: February 2022

Abstract

Aim
The primary aim was to examine the feasibility of intervention delivery and of trial procedures. Secondary aims were to study the intervention uptake; its acceptability and perceived utility; and its potential to improve safety culture and avoidable hospital admissions.

Methods
We conducted a 3-month, single-arm feasibility study in 10 primary care (PC) centres in Spain. Centres received information regarding patients’ experiences of safety (through the Patient Reported Experiences and Outcomes of Safety in Primary Care [PREOS-PC] questionnaire), and were instructed to plan safety improvements based on that feedback. We used a bespoke online tool to recruit PC professionals, collect patient feedback, and deliver it to the centres, and to collect outcome data (patient safety culture [Medical Office Survey on Patient Safety Culture, MOSPSC questionnaire]). We measured recruitment and follow-up rates and intervention uptake (based on the number of safety improvement plans registered). We conducted semistructured interviews with 9 professionals to explore the intervention acceptability and perceived utility.

Results
Of 256 professionals invited, 120 (47%) agreed to participate, and 97 completed baseline and postintervention measures. Of 780 patients invited, 585 (75%) completed the PREOS-PC questionnaire. Five of 10 centres (50%) designed an improvement plan, providing 27 plans in total (range per centre, 1–14). The intervention was perceived as a novel strategy for improving safety, although the healthcare professionals identified several factors limiting its acceptability and utility: lack of feedback at the individual professional level; potentially unrepresentative sample of patients providing feedback; and number of educational materials deemed overwhelming.

Discussion
It is feasible to deliver the proposed intervention so long as the identified limitations are addressed.
The effects of plate interface rheology on subduction kinematics and dynamics
Authors: Behr Whitney M , Holt Adam F , Becker Thorsten W , Faccenna Claudio 
Published: February 2022

SUMMARY
Tectonic plate motions predominantly result from a balance between the potential energy change of the subducting slab and viscous dissipation in the mantle, bending lithosphere and slab–upper plate interface. A wide range of observations from active subduction zones and exhumed rocks suggest that subduction interface shear zone rheology is sensitive to the composition of subducting crustal material—for example, sediments versus mafic igneous oceanic crust. Here we use 2-D numerical models of dynamically consistent subduction to systematically investigate how subduction interface viscosity influences large-scale subduction kinematics and dynamics. Our model consists of an oceanic slab subducting beneath an overriding continental plate. The slab includes an oceanic crustal/weak layer that controls the rheology of the interface. We implement a range of slab and interface strengths and explore how the kinematics respond for an initial upper mantle slab stage, and subsequent quasi-steady-state ponding near a viscosity jump at the 660-km-discontinuity. If material properties are suitably averaged, our results confirm the effect of interface strength on plate motions as based on simplified viscous dissipation analysis: a ∼2 order of magnitude increase in interface viscosity can decrease convergence speeds by ∼1 order of magnitude. However, the full dynamic solutions show a range of interesting behaviour including an interplay between interface strength and overriding plate topography and an end-member weak interface-weak slab case that results in slab break-off/tearing. Additionally, for models with a spatially limited, weak sediment strip embedded in regular interface material, as might be expected for the subduction of different types of oceanic materials through Earth’s history, the transient response of enhanced rollback and subduction velocity is different for strong and weak slabs. Our work substantiates earlier suggestions as to the importance of the plate interface, and expands the range of quantifiable links between plate reorganizations, the nature of the incoming and overriding plate and the potential geological record.
Multi-omics approaches identify a key gene, PpTST1, for organic acid accumulation in peach
Authors: Wang Qi , Cao Ke , Cheng Lailiang , Li Yong , Guo Jian , Yang Xuanwen , Wang Jiao , Khan Irshad Ahmad , Zhu Gengrui , Fang Weichao , Chen Changwen , Wang Xinwei , Wu Jinlong , Xu Qiang , Wang Lirong 
Published: February 2022

Abstract
Organic acid content in fruit is an important determinant of peach organoleptic quality, and undergoes considerable variations during development and maturation. However, its molecular mechanism remains largely unclear. In this study, an integrative approach of genome-wide association studies and comparative transcriptome analysis was applied to identify candidate genes involved in organic acid accumulation in peach. A key gene, PpTST1, encoding tonoplast sugar transporter, was identified and the genotype of PpTST1 with a single-base transversion (G1584T) in the third exon that leads to a single amino acid substitution (Q528H) was associated with a low level of organic acid content in peach. Overexpression of PpTST1His resulted in reduced organic acid content along with increased sugar content both in peach and tomato fruits, suggesting its dual function in sugar accumulation and organic acid content reduction. Two V-type proton ATPases interacted with PpTST1 in a yeast two-hybrid assay. In addition, the G1584T transversion appeared and gradually accumulated during domestication and improvement, which indicated that PpTST1 was under selection. The identification and characterization of PpTST1 would facilitate the improvement of peach fruit quality.
The circadian clock and cancer: links between circadian disruption and disease Pathology
Authors: Fekry Baharan , Eckel-Mahan Kristin 
Published: February 2022

Abstract
There is growing evidence that disruption of our 24-h clock increases our risk for acquiring several diseases and disorders. One of these diseases is cancer. While the mechanistic links between circadian clock disruption and cancer initiation or progression are an active area of study, significantly more work needs to be done to understand the molecular substrates involved. Of particular complexity remains the functions of the clock in individual cells during the process of transformation (cancer initiation) versus the functions of the clock in tumour-surrounding stroma in the process of tumour progression or metastasis. Indeed, the nexus of cellular circadian dynamics, metabolism and carcinogenesis is drawing more attention, and many new studies are now highlighting the critical role of circadian rhythms and clock proteins in cancer prevention. In this brief review, we cover some of the basic mechanisms reported to link circadian disruption and cancer at the level of gene expression and metabolism. We also review some of the human studies addressing circadian disruption and cancer incidence as well as some controlled laboratory studies connecting the two in pre-clinical models. Finally, we discuss the tremendous opportunity to use circadian approaches for future prevention and treatment in the context of cancer in specific organs.
Hanseniaspora uvarum Attracts Drosophila suzukii (Diptera: Drosophilidae) With High Specificity
Authors: Kleman Isabella , Rehermann Guillermo , Kwadha Charles A , Witzgall Peter , Becher Paul G 
Published: February 2022

Abstract
Since the early phase of the intercontinental dispersal of Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), fermentation baits have been used for monitoring. Self-made lures and commercial products are often based on wine and vinegar. From an ecological perspective, the formulation of these baits is expected to target especially vinegar flies associated with overripe fruit, such as Drosophila melanogaster (Meigen) (Diptera: Drosophilidae). Hanseniaspora uvarum (Niehaus) (Ascomycota: Saccharomyceta) is a yeast closely associated with D. suzukii and fruit, and furthermore attractive to the flies. Based on this relation, H. uvarum might represent a suitable substrate for the development of lures that are more specific than vinegar and wine. In the field, we therefore, compared H. uvarum to a commercial bait that was based on vinegar and wine with respect to the number of trapped D. suzukii relative to other drosophilids and arthropods. Trap captures were higher with the commercial bait but specificity for D. suzukii was greater with H. uvarum. Moreover, H. uvarum headspace extracts, as well as a synthetic blend of H. uvarum volatiles, were assayed for attraction of D suzukii in a wind tunnel and in the field. Headspace extracts and the synthetic blend induced strong upwind flight in the wind tunnel and confirmed attraction to H. uvarum volatiles. Furthermore, baited with H. uvarum headspace extract and a drowning solution of aqueous acetic acid and ethanol, 74% of field captured arthropods were D. suzukii. Our findings suggest that synthetic yeast headspace formulations might advance the development of more selective monitoring traps with reduced by-catch.