bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNAs ENST 00000499459 and TCONS_00004989 enhance asthma progression in children with house dust mite-induced allergic asthma
Authors: Zhang Xu-De , Feng Shao-Jie , Zhang Xi-Yan , Liu Jing-Jing , Liu Feng-Xia 
Published: July 2022

Abstract
Background
Long noncoding RNAs (lncRNAs) have been extensively reported to play critical roles in disease pathogenesis, especially in the field of cancer. However, there has been limited research on the role of lncRNAs in the pathogenesis of pediatric allergic asthma.
Methods
High-throughput sequencing analysis was performed to identify differentially expressed mRNAs and lncRNAs in peripheral blood mononuclear cells (PBMCs) from 3 children with allergic asthma and 3 matched healthy controls. Bioinformatics analysis was used to select candidate lncRNAs and mRNAs that may be involved in the pathogenesis of asthma. Candidate lncRNAs were used for validation in a larger sample of asthma patients and healthy controls. Finally, lncRNAs and molecular pathways associated with the pathogenesis of allergic asthma were identified by competing endogenous RNA (ceRNA) analysis.
Results
Five differentially expressed lncRNAs (DE lncRNAs) were identified after high-throughput sequencing and verified by real-time PCR. LncRNAs ENST0000631797, TCONS_00004989 and ENST00000499459 were verified to have significant differences in expression among the PBMC samples from 15 AAS patients and 15 healthy controls. ENST00000499459/DIXDC1 was finally identified as a marker molecule of allergic asthma after comprehensive ceRNA network analysis.
Conclusion
ENST00000499459 and TCONS_00004989 are potential biomarkers in children with house dust mite-induced allergic asthma.
The role of platelet count in predicting out-field occurrence of hepatocellular carcinoma patients receiving stereotactic body radiation therapy
Authors: Jia Jun , Sun Jing , Li Dong , He Wei-ping , Zhang Dan , Li Wen-gang , Duan Xue-zhang 
Published: July 2022

Abstract
Background
To explore the value of the pretreatment platelet (PLT) count in patients with naïve small hepatocellular carcinoma (HCC) treated with Cyber-Knife stereotactic body radiation therapy (CK-SBRT).
Methods
A total of 4637 consecutive HCC patients whose single lesion with a diameter ≤ 5 cm were treated with CK-SBRT in our center. After screening, survival outcomes were analyzed in 490 enrolled patients, and radiation-induced liver disease (RILD) was analyzed in 435 patients.
Results
A lower pretreatment PLT count was found to be associated with worse overall survival rates (HR 0.991, 95% CI 0.987–0.995, p < 0.001*), progression-free survival rates (HR 0.996, 95% CI 0.993–0.998, p < 0.001*), lower distant metastasis-free survival rates (HR 0.993, 95%CI 0.991–0.996, p < 0.001*) and higher occurrence rates of RILD (HR 0.983, 95% CI 0.973–0.993, p = 0.001*).
Conclusions
The pretreatment platelet count should be given more attention before CK-SBRT in HCC patients, and the platelet could predict out-field recurrence and radiation induced liver injury.
The sperm-interacting proteome in bovine oviduct fluid: temporal and spatial resolution
Authors: Mahé Coline , Lavigne Régis , Com Emmanuelle , Pineau Charles , Zlotkowska Aleksandra Maria , Guillaume Tsikis , Mermillod Pascal , Schoen Jennifer , Saint-Dizier Marie 
Published: July 2022

Abstract
Background: Spermatozoa interact with oviduct secretions before fertilization in vivo but the molecular players of this dialog and underlying dynamics remain largely unknown. Our objectives were to identify an exhaustive list of sperm-interacting proteins (SIPs) in the bovine oviduct fluid and to evaluate the impact of the oviduct anatomical region (isthmus vs. ampulla) and time relative to ovulation (pre-ovulatory vs. post-ovulatory) on SIP number and abundance. Methods: Pools of oviduct fluid (OF) from the pre-ovulatory ampulla, pre-ovulatory isthmus, post-ovulatory ampulla, and post-ovulatory isthmus in the side of ovulation were collected from the slaughterhouse. Frozen-thawed bull sperm were incubated with OF or phosphate-buffered saline (control) for 60 min at 38.5°C (4 replicates per condition). After protein extraction and digestion, sperm and OF samples were analyzed by nanoLC-MS/MS and label-free protein quantification. Results: A quantitative comparison between proteins identified in sperm and OF samples (2,333 and 2,471 proteins, respectively) allowed for the identification of 245 SIPs. The highest number (187) were found in the pre-ovulatory isthmus, i.e. time and place of the sperm reservoir. In total, 41 SIPs (17%) were differentially abundant between stages in a given region or between regions at a given stage and 76 SIPs (31%) were identified in only one region × stage condition. Functional analysis of SIPs predicted roles in cell response to stress, regulation of cell motility, fertilization, and early embryo development. Conclusion: This study provides a comprehensive list of SIPs in the bovine female genital tract and evidences dynamic spatio-temporal changes in sperm-oviduct interactions around ovulation time. Moreover, these data provide protein candidates to improve sperm conservation and in vitro fertilization media.
Effectiveness of Horizontal Sub-drain to Increase Slope Stability with Vertical Cracks and Weathering Soil in Areas with High Rainfall Intensity
Authors: sari Putu tantri Kumala , Mochtar Indrasurya B. , Chaiyaput Salisa 
Published: July 2022

Abstract
A landslide occurred a few meters from the foot of the power plant tower in a hilly area in the Tulakan District, Pacitan, East Java Province, Indonesia. This incidence occurred after the area was flooded with a cumulative intensity of more than 1000 mm in one month and a maximum daily rainfall above 300 mm. Previous reports considered horizontal sub-drain cost-effective to reduce the groundwater level and the impact of rainwater seepage into the soil on slope stability. Therefore, this study aims to determine the effectiveness of horizontal sub-drain as an alternative for managing groundwater and rainwater that seeps into the soil to increase the slope factor of safety. The effect of real-time rainfall for 30 days before the landslide, hydraulic conductivity, changes in soil parameters due to cracking and weathering, and the existing groundwater level are also considered. The analyses were carried out using the coupled program SEEP/W for transient seepage and SLOPE/W with finite element method and limit equilibrium. The result showed that horizontal sub-drain increases the slope factor of safety from critical limit to more than 1.1 in 30 days of rainfall in high ground water level condition. The hydraulic conductivity parameters of the soil, the location of the horizontal sub-drain, as well as the cracked and weathered conditions in the soil also affect the effectiveness. In low-level ground water conditions, the duration of the rain and the intensity of the rain also greatly affect the effectiveness of the horizontal sub-drain as well as the number of cracks. In this condition, the safety factor on the slope with the horizontal sub-drain can increase the safety factor from less than one to more than one thus the slope becomes more stable. The results of this study also show that the effectiveness of the horizontal sub-drain is very sensitive to many things, therefore in its planning it is necessary to review many aspects more carefully.
Staple Food Diversity From Palembang, Indonesia
Authors: NILAWATI NURUL SALASA , TANZIHA IKEU , SUKANDAR DADANG , PALUPI ENY 
Published: July 2022

Abstract
This study aimed to examine recipes, processing methods, and nutritional contents of staple food diversity from Palembang, Sumatera – Indonesia which is easy to be developed and has the potential for improving the food and nutrition security of the community. Data had been collected by interviewing 48 people from Gandus District, Palembang City. Analysis of nutritional value was carried out to see the nutritional content of the food. A total of 9 traditional Palembang staple foods have been explored and evaluated. The most preferred and frequently consumed food was pempek lenjer (75%), followed by pempek lenjer, pempek adaan, and pistel , 47.9, 25, and 2.1%, respectively. The highest carbohydrate content was pempek lenjer and pempek pistel , 30.28 and 30.36 g respectively. Based on this study, it was revealed that some traditional foods from Palembang indeed enable to be consumed as a source of carbohydrates alternatives. These foods also have a quality nutrient profile that might support the food and nutrition security of the community.
Enhance energy supply of largemouth bass (Micropterus salmoides) in gills during acute hypoxia exposure
Authors: Liu Qiao , Wang Hong , Ge Jiayu , Luo Jie , He Kuo , Yan Haoxiao , Zhang Xin , Tahir Rabia , Luo Wei , Li Zhiqiong , Yang Song , Zhao Liulan 
Published: July 2022

Abstract
Gills are the location of gas exchange and also the first target organ of fish response for environmental stress. As a multifunctional organ, its energy supply, when faced with insufficient dissolved oxygen in the water, remain unclear. In this study, largemouth bass were subjected to hypoxia stress (1.2 mg/L) for 24 h and 12 h reoxygenation (R12) to evaluate energy supply strategy of gills. Under hypoxia, gills respond firstly and rapidly by increasing respiratory rate and changing gene expression. Transcriptome data showed that 2026 differential expressed genes (DGEs) were detected in the early stage of hypoxia exposure, including genes related to oxygen sensor and transport. KEGG functional analysis of DEGs revealed that the glycolysis/gluconeogenesis pathway was enriched across all the sampling points (0, 4, 8, 12, 24h, R12). The gene expression and enzyme activity of three rate-limiting enzymes (hexokinase, phosphofructokinase-6, pyruvate kinase) in glycolysis pathway were significantly increased. The increase of glycolysis products pyruvate and lactic acid, as well as the number of mitochondria, suggest the enhancement of aerobic and anaerobic metabolism of glucose. These results suggest that gill of largemouth bass enhanced energy supply during acute exposure to hypoxia stress.
Visible-light-responsive Ag/TiO2/PVA nanocomposite for photocatalytic degradation of Cr, Ni, Zn, and Cu heavy metal ions
Authors: Dehghani Mohammadtaha , Delnavaz Mohammad 
Published: July 2022

Abstract
The rapid growth of industrialization has led to the uncontrolled pollution of the environment, and rapid action is needed. This study synthesized Ag/TiO2/polyvinyl alcohol (PVA) nano photocatalyst for promising light-derived photocatalytic removal of heavy metal ions. The design of experiment (DOE) was used to study the effect of important factors (pH, reaction time, and photocatalyst dosage) to maximize the final performance of the photocatalyst. In the optimized condition, the Ag/TiO2/PVA nano-photocatalyst removed more than 94% of Cr6+ in 180 minutes, and the efficiency was more than 70% for Cu2+, Zn2+, and Ni2+ metal ions. The adsorption of the heavy metal ions on the photocatalyst was described well with the Langmuir isotherm, while the pseudo-second-order linear kinetic model fitted with the experimental data. The nano-photocatalyst's stability was confirmed after maintaining its performance for five successive runs. The enhanced photocatalytic activity for the heavy metal ions removal can be attributed to the presence of metallic silver nanoparticles (electron transfer and plasmonic fields mechanisms) and PVA, which delayed the recombination of electron-hole. The synthesized ternary Ag/TiO2/PVA nano-photocatalyst showed promising performance for the elimination of heavy metal ions and can be used for environmental remediation purposes.
Attenuating the virulence of the superbug Staphylococcus aureus by sodium bicarbonate, dexamethasone, and ascorbic acid
Authors: Saleh Moustafa M. , Yousef Nehal , Shafik Shokri M. , Abbas Hisham A. 
Published: July 2022

Abstract
Background: Infections affecting neonates caused by Staphylococcus aureus are widespread in healthcare facilities; hence, novel strategies are needed to fight this pathogen. Targeting virulence factors necessary to induce host damage and disease is a new tactic for antimicrobial therapy with several benefits which could lead to decreased resistance. The effect of FDA-approved drugs (sodium bicarbonate, dexamethasone, and ascorbic acid) with sub-inhibitory concentration (1/8 MIC) was evaluated for their capability to hinder virulence factors production in Staphylococcus aureus phenotypically. Furthermore, their effects were estimated genotypically using qRT‑PCR.Results: Sub-inhibitory concentrations (1/8 MIC) of sodium bicarbonate, dexamethasone, and ascorbic acid reduced the production of Staphylococcus aureus virulence factors, including biofilm formation, staphyloxanthin, proteases, and hemolysin production, as well as resistance to oxidative stress. At the molecular level, qRT-PCR was used to assess the relative expression levels of CrtM, SigB, SarA, AgrA, hla, fnbA, and icaA genes regulating virulence factors production and showed a significant reduction in the relative expression levels of all the tested genes. Conclusions: In summary, the current findings reveal that sodium bicarbonate, dexamethasone, and ascorbic acid have strong anti-virulence effects against multi-drug resistant Staphylococcus aureus. Thus, suggesting the use of sodium bicarbonate, dexamethasone, and ascorbic acid for fighting Staphylococcus aureus, and also suggesting that they might be used to treat infections caused by multi-drug resistant Staphylococcus aureus in place of or in addition to conventional antimicrobials.
Slow and incomplete recovery of gut microbiome in Clostridioides difficile patients treated with metronidazole
Authors: Antharam Vijay C , Mesbah Zhubene , Zori Andreas , Kozlov Andrew , Zhao Lisa , Santos Janelle , Li Eric , Wang Gary P. 
Published: July 2022

Abstract
Background: Clostridioides difficile infection (CDI) causes 3 million cases of diarrhea and colitis in the US annually. Although antibiotic therapy is effective in suppressing C. difficile, up to 30% of treated patients experience recurrences. Recovery of gut microbiota may be important for sustained resolution of C. difficile diarrhea and achieve clinical cure. However, the extent and the dynamics of microbiome recovery following C. difficile therapy remains poorly understood. Methods: Fecal samples from 25 C. difficile patients treated with metronidazole and 14 healthy controls were collected prospectively over 3 months and analyzed using 16S rRNA sequencing and bioinformatic methods. Results: 19 of 25 patients (76%) achieved clinical cure and 6 (24%) developed recurrent CDI. Analysis of 448 longitudinal fecal samples revealed that the recovery of gut microbiome in patients who achieved clinical cure was slow and incomplete. In most patients, microbiome recovery was accompanied by increased diversity, richness and abundance of Lachnospiraceae and Ruminococcaceae. However, the microbiome composition and structure in most patients did not resemble that of healthy controls at the end of the study. Microbiome functions associated with amino acid and nitrogen metabolism were enriched during CDI, but not at clinical cure, suggesting their role in CDI. In patients who developed recurrent CDI, the recovery of gut microbiome was variable and individualized.Conclusions: Resolution of C. difficile diarrhea and clinical cure is characterized by slow but incomplete recovery of Lachnospiraceae and Ruminococcaceae. Microbiome functions associated with amino acid and nitrogen metabolism may play a role in C. difficile pathogenesis.
Coral Reproduction in a Changing Climate
Authors: Bouwmeester Jessica , Daly Jonathan , Zuchowicz Nikolas , Lager Claire , Henley E. Michael , Quinn Mariko , Hagedorn Mary 
Published: July 2022

Abstract
Coral reefs worldwide are at risk due to climate change. Coral bleaching is becoming increasingly common and corals that survive bleaching events can suffer from temporary reproductive failure for several years. While water temperature is a key driver in causing coral bleaching, other environmental factors are involved, such as solar radiation. We investigated the individual and combined effects of temperature, photosynthetically active radiation (PAR), and ultraviolet radiation (UVR) on the spawning patterns and reproductive physiology of the Hawaiʻian mushroom coral Lobactis scutaria, using long-term experiments in aquaria. We examined effects on spawning timing, fertilisation success, and gamete physiology. Both warmer temperatures and filtering UVR altered the timing of spawning. Warmer temperatures caused a drop in fertilisation success. Warmer temperatures and higher PAR both negatively affected sperm and egg physiology. Thermal stress from global climate change will need to be adequately addressed to ensure the survival of reef-building corals in their natural environment throughout the next century and beyond. Until then, reproduction is likely to be increasingly impaired. On a regional scale, some damage related to photo-oxidative stress may be partially mitigated through shading reefs or specific corals, to reduce bleaching, minimise the loss of coral fitness, and maintain coral reproduction.
Contribution of coronal vertebral and IVD wedging to Cobb angle changes in Adolescent Idiopathic Scoliosis during growth
Authors: Cheung Wing Ki , Cheung Jason Pui Yin 
Published: July 2022

Abstract
BackgroundVertebral and intervertebral disc (IVD) wedging are often seen in patients with adolescent idiopathic scoliosis (AIS). However, the relationship between wedging and curve progression, and the change of wedging before bracing to final weaning is unknown. The aim of this study was to investigate the pattern and sequence of vertebral and IVD wedging development, and to determine the relationship between the change of wedging and curve progression in AIS during growth.MethodsThis was a prospective study of 32 AIS females with right-sided thoracic curves and/or left-sided lumbar curves who completed brace treatment. They were classified into progression and non-progression groups. Vertebral and IVD wedging were calculated for each spinal segment. The wedging pattern was first identified and then used to determine the sequence of wedging development. Percentage change in the sum of wedging during growth was calculated and compared.ResultsThe sum of vertebral wedging for both groups was 2.4° to 8.7° more than that of IVD wedging in the thoracic spine but 8.7° to 17.7° less in the lumbar spine. Out of the 20 curves assessed, 5 thoracic curves and 1 lumbar curve developed vertebral wedging before IVD wedging, and 3 thoracic curves and 4 lumbar curves had the opposite pattern. The progression group had larger increases in sum of vertebral (40%) and IVD (28.6%) wedging as compared to the non-progression group (both 16.7%). A significant difference in wedging between the first and the latest visits was found in the progression group only (p<0.05).ConclusionPattern and sequence of vertebral and IVD wedging were related to the location of the curve rather than the presence of curve progression. Progressed curves were associated with increasing wedging during growth.
Exploring facilitators and barriers to self-management engagement of Chinese people with type 2 diabetes mellitus and poor blood glucose control: A descriptive qualitative study
Authors: LIU YUAN , Jiang Jiajia , You Wenjun , Gong Dandan , Ma Xiaoqing , Wu Min , Li Feng 
Published: July 2022

Abstract
Background: Blood glucose is poorly controlled and self-management engagement is inadequate in Chinese people with type 2 diabetes. This study aimed to explore facilitators and barriers to self-management engagement of Chinese people with poorly controlled type 2 diabetes.Methods: Patients wit poorly controlled type 2 diabetes were recruited from National Metabolic Management Center. Purposive sampling method was used for recruitment. Semi-structured interview and thematic analysis was used for data collection and analysis. Results: 26 semi-structured interviews were conducted. Poor blood glucose increased awareness of susceptibility to complications, which motivated self-management engagement. However, emotional burdens could be concomitant. General knowledge about lifestyle management was useless for successful lifestyle management engagement. Unhealthy habits impeded lifestyle management engagement. Temporary remission of hyperglycemia and no perceived physical symptoms interfered engagement of medication therapy and regular blood glucose monitoring. Family and work environment could impact self-management engagement. Accessibility to reliable diabetes-related information could also influence self-management engagement.Conclusions: Increasing patients’ awareness of poor blood glucose status and susceptibility to complications is essential. However, concomitant emotional burdens could be concomitant. Formulating customized lifestyle management plans should be considered to replace providing general knowledge. Behavior change technologies should integrate in current Chinese diabetes services. Diabetes educators should emphasize the natural progression of diabetes and the importance of medication therapy continuity. Furthermore, the value of blood glucose monitoring should be clarified. Policies on building diabetes-friendly family and work environments should be considered. Building a channel that provides reliable diabetes-related information and is accessible in routine life is essential.
Physiotherapists’ Knowledge of and Adherence to Evidence-Based Practice Guidelines and Recommendations for Ankle Sprains Management: a Cross-Sectional Study
Authors: Caffini Giulia , Battista Simone , Raschi Andrea , Testa Marco 
Published: July 2022

Abstract
Background
Lateral ankle sprain (LAS) is a common and burdensome injuries. However, the quality of its management is scant. Nowadays, physiotherapy management of musculoskeletal diseases seems to be generally not based on research evidence. Studies that investigated the knowledge-to-practice gap in LAS management are yet to be carried out. Therefore, this research investigated physiotherapists’ knowledge of and adherence to LAS Clinical Practice Guidelines (CPGs) and recommendations.
Methods
A cross-sectional study based on an online survey built on the LAS CPGs 2013 updated in 2018 and in 2021 and the recommendations of the 2018 Consensus Statement. The survey was structured in three sections. The first section collected demographic data. Second section showed two clinical cases (with positive and negative Ottawa Ankle Rules (OAR), respectively). The participants indicated which treatments they would adopt to manage them. Participants were classified as ‘following’, ‘partially following’, ‘partially not following’ and ‘not following’ the CPGs and recommendations. In the third section, participants expressed their agreement with different CPG and recommendation statements through a 1 (completely disagree) to 5 (completely agree) scale. We defined a ≥ 70% agreement with a statement as consensus.
Results
In total, 483 physiotherapists (age: 34 ± 10; female 38%, male 61.5%, other 0.5%) answered the survey: 85% completed the first two sections, 76% completed all three sections. In a case of acute LAS with negative OAR, 4% of the participants were considering as ‘following’ recommended treatments, 68% as ‘partially following’, 23% as ‘partially not following’, and 5% as ‘not following’. In a case of acute LAS with positive OAR, 37% were considered ‘following’ recommended treatments, 35% as ‘partially following’, and 28% as ‘not following’. In section III, the consensus was achieved for 73% of the statements. These statements dealt with assessments choices, such as the need to use the OAR, and with treatments choices.
Conclusion
This study showed that although there is a good knowledge about first-line recommended treatments, a better use of CPGs and recommendations should be fostered among physiotherapists. Our results identify an evidence-to-practice gap in LAS management, which may lead to non-evidence-based practice behaviours.