bluuebunny/crossref_metadata_2025_split huggingface dataset:
Qualitative interviews with physicians: Overcoming barriers to access to bring telehealth to older adults during COVID-19
Authors: Li Melinda M. , Strauss Daniel H. , Chen Kevin , Davoodi Natalie M. , Joerg Lucie , Jimenez Frances , Goldberg Elizabeth M. 
Published: August 2022

Introduction While the COVID-19 pandemic increased telehealth uptake, concerns surrounding access to telehealth visits emerged for older adults, especially those that are under-resourced, rural, and non-English speaking. We aimed to identify strategies employed by physicians to improve access to medical care via telehealth for older adults during the pandemic. Methods Using purposeful sampling, we recruited physicians for 30-minute semi-structured interviews. The interviews focused mainly on telehealth’s usability for older patients, and specific strategies physicians employed to facilitate access to care for older adults with varied needs and skills. Interviews were coded using NVivo software and analyzed using framework matrix analysis. Results Of 48 physicians (geriatrician n = 18, primary care n=15, emergency n=15) interviewed, median age was 37 (interquartile range 34-45), and 27 (56%) were women. Our data suggests that older adults who were under-resourced, rural, or non-English speaking faced greater challenges in using telehealth. Physicians addressed these barriers with strategies tailored to individual patient needs and practice setting resources. Strategies included employing auditory augmentation and speech modifications to enhance understanding, using caregivers and home services to overcome inexperience with navigating technology, device distribution programs for those without equipment access, and real-time interpreter technologies. Physicians reported that telehealth was successful in reducing transportation barriers, whether due to distance or mobility impairments, especially among older adult populations and reducing “no-show” appointment rates. Conclusions Adoption of strategies to overcome technological and systemic barriers may improve equitable access to age-friendly telehealth for older adult patients. Physicians reported optimism that telehealth could be used to expand access to medical care for older adults with the right technological infrastructure, payment models, and support. Although challenges remain, they may be overcome with additional technical training for clinicians and patients, investment in telehealth infrastructure and broadband internet, and policy changes to promote telehealth access for this diverse patient population.
FDI flow in Energy Sector among BCIM, BIMSTEC+1 and ASEAN+4 sub-regional alignments
Authors: Banerjee Kallal 
Published: August 2022

FDI is the most dynamic component for boosting trade among different sub-regional blocs. It may be considered under Mode 3 of the GATS agreement. This study documents various restrictions under market access and national treatments under mode 3 as committed by different member countries, among three regional trading blocs to improving the energy sector. A large portion of trade is being facilitated through strong linkage between service trade and FDI flow among countries or regions. ASEAN countries among South East Asia played an important role in attracting foreign direct investments for the economic development and growth in Energy Sector. Trade barriers, particularly commitment barriers of importing country, have a strong negative impact on smooth flow of investments across regions. This research also reviews the purposes of FDI among regional countries and explores the deepest and strong relationships among regional members to explore a possible strategic relationship for formation of Regional Trade Agreement (RTA).
Psychosocial Problems Associated with Vitiligo: A Correlational Study on Vitiligo Patients
Authors: Ejaz Hira , Hassan Zia Mubashar , Hafiz Beenish , Khan Iqbal Ahmad , Javeria Sadaf 
Published: August 2022

Psychosocial problems influence the mental health as well as daily living of individuals. Vitiligo is one of those diseases which are strongly associated with psychosocial problems among its victim. Individuals with this disease are found to experience marriage delay, workplace discomfort, anxiety, depression, and remained to fail to cope with their skin condition. To determine psychosocial problems among vitiligo cases and to evaluate the role of psychotherapy in case management of vitiligo patients, this study employed a quantitative correlational study design. Data was collected from skin clinics in Islamabad from 15th December 2017 to 15th April 20018. A sample of 100 respondents including 60 cases and 40 controls was selected through convenient sampling technique. Questionnaires on self-esteem, depression, and Dermatology life quality index (DLQI) were used for data collection. Descriptive and inferential analysis was performed for data analysis. Pearson correlation between vitiligo and psychosocial problems was significant at the p-value 0.01 level (2-tailed). Study results showed that vitiligo has a significant relationship with depression, self-esteem, and impaired quality of life among cases. Regression analysis among Vitiligo and psychosocial problems was significant with a p-value < .001. Moderation regression analysis between vitiligo effects and psychotherapy was not found significant. Vitiligo has been found associated with depression, low self-esteem, and impaired quality of life. However, the proposed effect of psychotherapy has not been confirmed. Therefore, the study suggests further research to evaluate the role of psychotherapy to manage psychosocial problems of vitiligo cases.
Keywords: Psychosocial, problems, associated, correlational study, vitiligo patients.
UDZIAŁ KOMÓREK IMMUNOLOGICZNYCH W PATOGENEZIE ORBITOPATII GRAVESA
Authors: Król Anna 
Published: August 2022

Choroba Gravesa i Basedowa (GD; ang. Graves’ disease) jest chorobą autoimmunologiczną powodującą nadprodukcję hormonów tarczycy, która skutkuje powstawaniem zaburzeń metabolicznych. Orbitopatia Gravesa (GO; ang. Graves’ orbitopathy) jest najczęstszym pozatarczycowym objawem GD. Obejmuje ona zapalenie i obrzęk mięśni gałki ocznej, jak również wzrost objętości tkanki łącznej w obrębie oczodołów. Analiza tkanek pobranych od pacjentów dotkniętych GO pozwoliła dostrzec szczególną rolę fibroblastów oczodołowych, limfocytów T oraz receptorów dla tyreotropiny (TSHR) i insulinopodobnego czynnika wzrostu 1 (IGF1R) w rozwoju tej patologii. Powstający naciek zapalny i nagromadzenie glikozaminoglikanów prowadzi do rozwoju obrzęku i włóknienia tkanek okołogałkowych. Niestety, pomimo postępu w rozumieniu tego patomechanizmu, GO ciągle pozostaje jednym z najtrudniejszych problemów endokrynologii klinicznej. W tej publikacji dokonano przeglądu badań dotyczących immunologicznych aspektów GO i omówiono rolę komórek immunologicznych w patogenezie choroby.
CHROMATOGRAFICZNE METODY IZOLACJI I IDENTYFIKACJI FLAWONOIDÓW I SAPONIN
Authors: Machowski Michał , Kaliszewska Dorota , Kiss Anna 
Published: August 2022

W ciągu kilku ostatnich lat liczne nowe metody chromatograficzne stały się dostępne w analizie chemicznej flawonoidów i saponin. Metody te nie tylko skracają czas rozdziału tych związków, lecz umożliwiają izolację wcześniej nieznanych lub niestabilnych składników ekstraktów surowców roślinnych. Flawonoidy i saponiny są głównymi bioaktywnymi związkami roślin, posiadającymi właściwości przeciwutleniające, przeciwbakteryjne i owadobójcze. Przedstawiony przegląd literaturowy omawia chromatograficzne metody izolacji i identyfikacji flawonoidów i saponin.
Evolution of Helicobacter spp: variability of virulence factors and their relationship to pathogenicity
Authors: Prada Carlos F. , Casadiego Maria A. , Freire Caio CM 
Published: August 2022

Background
Virulence factors (VF) are bacteria-associated molecules that assist to colonize the host at the cellular level. Bacterial virulence is highly dynamic and specific pathogens have a broad array of VFs. The genus Helicobacter is gram-negative, microaerobic, flagellated, and mucus-inhabiting bacteria associated with gastrointestinal inflammation. To investigate about their pathogenicity, several Helicobacter species have been characterized and sequenced. Since the variability and possible origin of VF in the genus are not clear, our goal was to perform a comparative analysis of Helicobacter species in order to investigate VF variability and their evolutionary origin.

Methods
The complete genomes of 22 Helicobacter species available in NCBI were analyzed, using computational tools. We identifyed gain and loss events in VF genes, which were categorized in seven functional groups to determine their most parsimonious evolutionary origin. After verifying the annotation of all VF genes, a phylogeny from conserved VF organized by Helicobacter species according to gastric Helicobacter species (GHS) or enterohepatic (EHS) classification was obtained.

Results
Gain and loss analysis of VF orthologous in Helicobacter ssp revealed the most possible evolutionary origin for each gene set. Microevolutionary events in urease and flagella genes were detected during the evolution of the genus. Our results pointed that acquisition of ureases and adherence genes and deletion of cytotoxins in some lineages, as well as variation in VF genes copy number, would be related to host adaptation during evolution of the Helicobacter genus. Our findings provided new insights about the genetic differences between GHS and EHS and their relationship with pathogenicity.
Morphology and cytochemical patterns of peripheral blood cells of tiger frog (Rana rugulosa)
Authors: Chen Xianxian , Wu Yu , Huang Lixin , Cao Xue , Hanif Misbah , Peng Fei , Wu Xiaobing , Zhang Shengzhou 
Published: August 2022

BackgroundTiger frog (Rana rugulosa) is a national second-class protected amphibian species in China with an important ecological and economic value. In recent years, due to excessive human hunting, pollution and habitat loss, the wild population of tiger frog has declined sharply. To protect wildlife resources, the artificial breeding of tiger frogs has rapidly developed in China. Diseases are increasing and spreading among tiger frogs due to the increasing scale of artificial farming. The blood examination is the most straightforward and less invasive technique to evaluate the animal health condition. Thus, it is essential to obtain the normal hematological indicators of tiger frogs. The objective of this study was to investigate the morphometry, microstructure and cytochemical patterns of peripheral blood cells in tiger frogs.MethodsThe number of blood cells in tiger frogs was counted on a blood count board, and the cell sizes were measured by a micrometer under light microscope. The morphology and classification of blood cells were studied by Wright-Giemsa staining, and the cytochemical pateerns was investigated by various cytochemical staining including periodic acid-Schiff (PAS), Sudan black B (SBB), peroxidase (POX), alkaline phosphatase (AKP), acid phosphatase (ACP), chloroacetic acid AS-D naphthol esterase (CAE) andα-naphthol acetate esterase (ANAE) staining.ResultsBesides erythrocytes and thrombocytes, five types of leukocytes were identified in tiger frogs: neutrophils, eosinophils, basophils, lymphocytes and monocytes. The mean erythrocyte, leukocyte and thrombocyte counts were 1.33 ± 0.15 million/mm3, 3.73 ± 0.04 × 104/mm3and 1.7 ± 0.01 × 104/mm3, respectively. Small lymphocytes were the most abundant leukocytes, followed by large lymphocytes, Neutrophils, eosinophils and monocytes, basophils were the fewest. Eosinophils were strongly positive for PAS, positive for SBB, POX, ACP, CAE, ANAE, while weakly positive for AKP staining; basophils were strongly positive for PAS, ACP, positive for SBB, CAE, weakly positive for ANAE, negative for AKP, POX staining; neutrophils were strongly positive for ACP, SBB, positive for PAS, POX, weakly positive for AKP, CAE and ANAE staining; monocytes were positive for PAS, SBB, ANAE, weakly positive for ACP, AKP, POX, CAE staining; large lymphocytes and thrombocytes were positive for PAS, ACP, weakly positive for ANAE, while negative for SBB, POX, AKP, CAE; small lymphocytes were similar to large lymphocytes, except for strongly positive for PAS and ACP staining.ConclusionsThe blood cell types and morphology of tiger frogs were generally similar to those of other amphibians, while their cytochemical patterns had some notable species specificity.Our study could enrich the knowledge of peripheral blood cell morphology and cytochemistry in amphibians, and provide baseline data for health condition evaluation and disease diagnosis of tiger frogs.
Etiology and Age Distribution of Syncope in Delta Area Patients
Authors: Saleh Moataz Ragab , Bayomy Mohamed , Elhefnawy Sozan Bayoumy , El-Amrousy Mahmoud Zaki 
Published: August 2022

Background: Syncope is a Total Loss of Consciousness (TLOC) due to cerebral hypo-perfusion, characterized by a rapid onset, short duration, and spontaneous complete recovery. It shares many clinical features with other disorders; it therefore presents in many differential diagnoses. This group of disorders is labelled TLOC. The aim of the present study was to determine the etiologies and the age distribution of patients with syncope in Delta area.
Methods: This study included thirty patients who had at least one episode of syncope. All patients in this study were subjected to history taking, clinical examination, laboratory investigations, standard 12-lead Electrocardiogram (ECG), Resting Transthoracic Echocardiography (TTE). Some patients were subjected to Prolonged ECG monitoring (Holter, Event, ILR), Brain computed tomography (CT) and Magnetic resonance imaging (MRI), Tilt table test and Electrophysiology study (EP study).
Results: Most common ECG rhythm was normal sinus rhythm, complete heart block, left ventricular hypertrophy, Slow Atrial fibrillation (AF), Left bundle branch block (LBBB), ventricular tachycardia. The most common diagnosis of syncope was vasovagal syncope, idiopathic syncope then cardiac syncope. Incidence of idiopathic syncope in populations under 40 years was significantly higher than in populations over 40 years, meanwhile regarding cardiac-related etiology for syncope in patients under age 40 was significantly lower than those patients over age 40.
Conclusion: For age distribution, the incidence of idiopathic syncope in populations under 40 years was significantly higher than in populations over 40 years, meanwhile cardiac-related etiology for syncope in patients under age 40 was significantly lower than those patients over age 40.
The Influence of River Filtration Water on the Nutritional Content of Red Spinach Plants (Amaranthus tricolor L)
Authors: Hartini Eko , Aryani Lenci , Khoironi Adian , Isworo Slamet 
Published: August 2022

Background: The cultivation of red spinach in urban farming is expected to meet the needs of people in urban areas for vegetable consumption. It is feared that using contaminated river water for irrigation will reduce the nutritional value of spinach plants. The purpose of this study was to determine the effect of water filtration on the nutritional content of Red Spinach plants (Amaranthus tricolor L).
Methods: This is an experimental study with a post test control group design.
Results: The results showed that the quality of the filtered water was superior to river water and met the Indonesian Government Regulation Attachment VI of the National Water Quality Standard, making it suitable for crop irrigation. The highest water content found in leaves with water filtration treatment was 86.464%, and the highest protein content in leaves with a value of 0.934% in the filtration water treatment, and the best fat content in leaves with a concentration value of 0.0631% in the filtration water treatment. The Fe, Ca, Vit A, and Vit C content remained relatively constant across the three treatments.
Conclusion: Filtered water treatment yields superior results and is more likely to be used to replace river water.
Hypertension and Lifestyle Modifications: Beyond Controversy
Authors: Chaudhary Bhupendra , Chaudhary Ansh 
Published: August 2022

Traditional approaches to controlling the epidemic of blood pressure-related atherosclerotic cardiovascular disease (ASCVD) in people with hypertension have mostly focused on pharmacological therapy. Nonetheless, nonpharmacologic therapy, commonly known as lifestyle modification, plays an essential and rising role in addition to medication therapy. Specifically, non-pharmacol­ogic therapies can serve as initial therapy in Stage 1 hyperten­sive patients, facilitate medication step down or withdrawal in patients with well-controlled hypertension, prevent hyper­tension in high-risk populations, and reduce blood pressure in normotensive individuals and thereby lower their risk of ASCVD.
It particularly refers to life-style modifications i.e. therapeutic lifestyle changes (TLC) which include reducing dietary sodium, exercise for at least 30 min per day, five days per week; to have Dietary Approach to stop Hypertension (DASH) diet protocol and to achieve a weight loss goal of 4.5 kg or more by non-pharmacological measures such as increased physical activity, reduced salt intake, reduced fat intake, alcohol abstinence, smoking cessation, behavioural changes, yoga, meditation etc.
In fact, when we talk about controlling of hypertension, lifestyle modifications is always the first step of foremost importance.
REALIDADES E INCERTEZAS NO NASCIMENTO DE UMA NOVA ERA E A RESPOSTA DO PENSAMENTO PROGRESSISTA
Authors: Baiardi Amílcar 
Published: August 2022

Este artigo discute as certezas e as incertezas do processo de globalização, bem como o papel do pensamento contemporâneo de esquerda. Tenta-se através do mesmo entender as tendências das transições econômicas, sociais, políticas e culturais provocadas pela mudança tecnológica. O argumento principal segue uma abordagem evolucionista, focalizando as amplas consequências da mudança tecnológica e seus impactos sobre todos os setores da vida humana. Como resultado de sua difusão, grandes ajustamentos estruturais são esperados. Realities and Doubts in the Birth of a New Era and the Answer of Progressionist Thought Amilcar Baiardi This paper discusses the predictability and the unpredicability in the globalization process as well as the role of contemporary leftist thought. There is an attempt to understand the present trends of economic social, political and cultural transitions produced by technical change. The main argument follows an evolutionary approach, focusing, on the widespread consequences of intensifying technical change and their consequent impacts on all sectors of human life. As a result of their diffusion, major structural adjustments are expected. Publicação Online do Caderno CRH: http://www.cadernocrh.ufba.br
A MÁQUINA FABULATÓRIA DA MÍDIA E O RE-ENCANTAMENTO DA POLÍTICA: A ESTRUTURA MÍTICA DAS CAMPANHAS ELEITORAIS MAJORITÁRIAS
Authors: Vasconcelos Accioly Carvalho Rejane 
Published: August 2022

O objetivo central do texto é abordar as conexões entre política e estrutura das narrativas míticas nas condições de vigência de uma cultura e sociabilidade vigorosamente mediatizadas. A hipótese central é que a máquina enunciativa da mídia opera cada vez mais pelo aciconamento da dimensão fabulosa e fabulatória do imaginário popular e não por um ideal de objetividade de reportar-se apenas a fatos e acontecimentos que lhe são exteriores. A tendência de re-encantamento do mundo pela exarcebação do nosso tráfico com as imagens contraria, assim, a famosa tese de Weber do desencantamento do mundo moderno pela técnica. As campanhas políticas majoritárias são tomadas como momentos privilegiados de investimento simbólico forte e portanto mítico, na construção das imagens públicas dos protagonistas que se colocam no centro da disputa pelo voto. The Fabulatory Machine of the Media and the Re-enchantment of politics: The Mythical Structure of Electoral Campaigns Rejane Vasconcelos Accioly Carvalho The central aim of the text is to tackle the connections between politics and the structure of mythical narratives in which a vigorously mediatized culture and sociability are in force. The central hypothesis is that the enunciative machine of the media operates more and more through the activation of the fabulous and fabulatory dimension of the popular consciousness and not through an ideal of objectivity in which only the facts and events which are exterior to it are reported. The tendency towards a re-enchantment of the world through the amplification of our exchange of images would oppose in this way Weber's famous thesis of the disenchantment of the modern world by technicism. Political campaigns are taken as moments privileged by strong symbolic investment and therefore mythical, in the construction of the public images of the protagonists that place themselves in the centre of the dispute for votes. The campaign of Tasso Jereissati for the government of the State of Ceará in 1986 is analysed as a model example of the approximation of politics to fabulous narratives concerning the confrontation of the forces of good and evil, in which everybody can participate thanks to the media. Publicação Online do Caderno CRH: http://www.cadernocrh.ufba.br
Fine Affinity Discrimination by Yeast Surface Display and Flow Cytometry
Authors: Van Antwerp Jennifer J. , Wittrup K. Dane 
Published: July 2002

AbstractYeast surface display is a eucaryotic system for the directed evolution of protein binding and stability. For antibody affinity maturation, achievable single‐pass enrichment factors are a critical variable. Both reliable recovery of rare clones (yield) and effective differentiation between clones of only slightly improved affinity (purity) are paramount. To validate yeast display's purification potential, trial sorting experiments were performed. The D1.3 (anti‐hen egg lysozyme) single chain variable fragment antibody and a 2‐fold higher affinity mutant (M3) were each displayed on the surface of Saccharomyces cerevisiae. M3‐displaying cells were mixed into the D1.3‐displaying cells at a ratio of 1:1000. Cells were fluorescently labeled according to antigen equilibrium binding and then sorted using a flow cytometer. Single‐pass enrichment of M3‐displaying cells was 125‐fold (± 65‐fold). This level of performance is achievable because of the precision and reproducibility of optimal labeling conditions. This work further demonstrates the capability of yeast display for very fine discrimination between mutant clones of similar affinity. Because large improvements in affinity typically result from combinations of small changes, this capability to identify subtle improvements is essential for rapid affinity maturation.
Geographic and seasonal variability of resistance to insecticides in Cacopsylla pyri L. (Hom., Psyllidae)
Authors: Buès R. , Boudinhon L. , Toubon J. F. , D’Arcier F. Faivre 
Published: July 2002

Pear psylla (Cacopsylla pyri L.) resistance to insecticides was studied in adults using the topical application method. In the Avignon population, the winter forms tested (diapausing generation) showed a greater tolerance to insecticide than the summer forms. High resistance rates, ranging from 25‐ to 180‐fold, were observed for three organo‐phosphorous insecticides. Resistance rates were seven times lower for pyrethrinoids and amitraz and practically nil for carbamates. Inter‐population resistance rates, for an identical period, were different for the three organo‐phosphorous insecticides and for deltamethrin in two localities. However, it was difficult to make comparisons in time due to seasonal variations.Rates of synergism by S,S,S‐tributyl phosphorotrithioate (DEF) and piperonyl butoxide (PBO) were low when applied 4 h before the insecticide (0.3‐ to 3.3‐fold), except for deltamethrin with PBO (>60‐fold). The presence of mixed‐function oxidases in winter forms is discussed with regard to bibliographical data concerning another psylla species from North America (Psylla pyricola Foerster).