bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface preparation effects on anodization and corrosion resistance of pure titanium grade 2
Authors: Buddhasuwan Panisara , Khamwannah Jirapon , Lothongkum Gobboon 
Published: February 2022

Abstract
A layer of TiO2 nanotubes is widely used as a surface application on Titanium (Ti) implants because the nanotubes help promote cell adhesion and provide better cell response than the metal surface. Although several studies have reported the relationship between TiO2 nanotubes and cell osseointegration rate, the corrosion rate of the TiO2 nanotube layer under the circumstance of usage has yet to be fully evaluated. In this work, we have studied the effect of surface preparation conditions on the corrosion behavior of a TiO2 nanotube layer on a pure Ti substrate. The surface of the titanium substrate was prepared by different methods, followed by one-step anodization to obtain a TiO2 nanotube array. A surface roughness non-contact tester and field emission scanning electron microscope were used to characterize the surface roughness and surface morphology of the as-received, mechanically polished, and chemically polished substrate both pre and post anodization. The corrosion behavior of the prepared substrates was also investigated in Phosphate Buffered Saline solution using potentiodynamic polarization technique. The results suggest that surface roughness at the nanoscale of the prepared surfaces has no relationship with corrosion resistance. However, after anodization, the corrosion rate has a good relationship with the standard deviation of inner tube/pore diameter.
Effects of laser and electron beam welding on the mechanical properties of bake hardening sheets
Authors: Özkul İskender , Şehirli Servet , Akkurt Adnan 
Published: February 2022

Abstract
Bake hardening sheets are frequently used materials in the automotive industry. The unique feature of these materials like hardening with the paint baking process is convenient for the manufacturer. Also, the bonding technique is a common issue for assembly lines. With new technologies regarding the welding process after the 1990s, the concept of welding has changed. In this study, the electron beam and laser beam sources, which are still considered new, are used for welding 260 BH bake hardening sheets used in the automotive industry as galvanized and non-galvanized. The characterization analysis of the mechanical behavior of the bonded materials was conducted. The macroscopic and microscopic structural modifications, as well as residual stresses, were measured, and they were compared with each other. From the experiments, very effective results have been found. The results were analyzed in detail from different perspectives in terms of material properties and types of weldment processes.
Multi-energy X-ray CT and data-constrained modeling of shale 3D microstructure
Authors: Li Jianli , Yang Yu-Shuang , Wang Haipeng , Yu Lingjie , Liu Keyu , Ma Jie , Wei Yadong 
Published: February 2022

Abstract
Three Longmaxi Formation shale samples from different well depths were analyzed for their total organic carbon (TOC) content and mineral phases. Synchrotron-based multi-energy X-ray computed tomography (CT) slices have been acquired for these samples. Deviations of the sample center away from the harmonic trajectory during X-ray CT imaging were corrected to improve the accuracy of CT reconstructed tomographic slices. The three-dimensional (3D) distribution of porosity and mineral in the samples was derived using the data-constrained modeling (DCM) method. The equivalent spherical diameters of connected pore-organics clusters and the connection probabilities were calculated to evaluate the spatial agglomeration and the spatial correlation of pore-organics in the samples. Numerical results showed that the distribution of the connected regions size and the connection probabilities of pore-organics for three samples have similar characteristics. The connection probabilities versus the distance follow exponential law. The connection probability appeared to be positively correlated to the number of pore-organics connected clusters when the distance between the voxels is shorter than 10 μm. Comparing samples from the three well depths, both the numbers of connected regions and connection probabilities of the samples from a deeper well are higher. The approach would be applicable for structural characterization of other similar materials.
Platform Configuration: A Longitudinal Study and Conceptualization of a Legacy News Publisher’s Platform-Related Innovation Practices
Authors: Chua Sherwin , Westlund Oscar 
Published: February 2022

Abstract

Purpose
Publishers are innovating their practices in the face of global platform companies’ growing dominance on journalism. This study examines how publishers innovate their editorially oriented activities vis-à-vis third-party platforms with respect to six stages of news production. In doing so, this article introduces and advances platform configuration as a conceptual framework.

Design/methodology/approach
This five-year longitudinal case study of a Singaporean legacy news publisher uses a mix-method qualitative approach. It includes in-depth interviews with 35 staff, newsroom observations and close monitoring of the publisher’s website and apps.

Findings
This study offers three key findings about the publisher’s platform configuration. First, multidirectionality: the publisher simultaneously leveraged on platforms’ capacities (building platform presence), while also reducing dependence on them (platform counterbalancing). Second, specificity: the publisher added, removed and/or modified editorially oriented activities with respect to the six stages of news production. Third, commitment: the publisher calibrated its commitment to specific activities oriented towards either building platform presence and/or platform counterbalancing.

Practical implications
This article introduces a 2 × 2 platform configuration matrix that classifies and explains how and why publishers engage in platform configuration.

Theoretical and social implications
Scholars can draw on platform configuration to study and advance theorizing on the evolving publisher-platform interrelationship. Platform configuration is useful for understanding how publishers reconcile their innovation practices and strategize their commitment to news activities in relation to platforms with broader journalistic and financial objectives.

Originality/value
This is the first study that introduces and advances the concept of platform configuration with regard to publishers’ innovation practices. Both the platform configuration concept and matrix allow researchers to classify and operationalize future longitudinal and short-term studies into the publisher-platform dynamic.
Modeling analysis of microenvironment of 3D cell mechanics based on machine vision
Authors: Zhang Yuejin , Ye Mengqiu , Wang Juan , Li Guanghui , Zhong Meiling , Zhan Aiyun 
Published: February 2022

Abstract
Aiming at the problem of poor construction accuracy of the cellular three-dimensional (3D) mechanical microenvironment, this article studies the cellular 3D mechanical microenvironment based on machine vision. The gelatin methacrylate microgel column was prepared by NIH/3T3 mouse fibroblast and precursor solution of gelatin methacrylate microgel. The gelatin methacrylate microgel array with magnetic end was adopted. The external magnetic field was used to load microgel array and build 3D mechanics microenvironment model. The deformed pictures of hydrogel under magnetic field were obtained by fluorescence microscope. The scanning electron microscope was used to characterize the pore structure of gelatin methacrylate hydrogel. The pictures obtained by machine vision method were used to calculate the deformed parameters of sample. The machine vision method adopted the discrete cosine transform for autofocus, and then used the image analysis and processing technology to identify and estimate the cell motion parameters. After getting the cell motion parameters, Comsol multiphysics (COMSOL) multiphysics multifield coupling finite element analysis software was adopted. The correlative numerical simulation method and gel deformed simulation method were used to obtain the mechanical changes of cells in the 3D mechanical microenvironment. Experimental results show that the modulus of gelatin methacrylate microgel is changed significantly during the tensile loading. The tensile strain and the cell spreading area are nonlinearly related. The increase in stiffness of the hydrogel substrate helps to promote cell proliferation to a certain extent.
Efficient data aggregation technique for medical wireless body sensor networks
Authors: Belhaj Mohamed Mbarka , Meddeb-Makhlouf Amel , Fakhfakh Ahmed , Kanoun Olfa 
Published: February 2022

AbstractA central issue in Wireless Body Sensor Networks (WBSNs) is the large amount of measurement data for monitoring vital parameters, which need to be continuously measured, immediately processed and timely transmitted. This requires a big storage space and computing effort leading to a high-power consumption. Reducing the amount of transmitted data contributes significantly to an extension of the sensor operation time. In this contribution, we focus exactly at this aspect. We propose a data aggregation method based on Artificial Neural Networks (ANN) combining multiple physiological signals, which are the ElectroCardioGram (ECG), ElectroMyoGram (EMG) and Blood Pressure (BP), in one signal before transmission. The simulation and implementation results reveal a reduction of energy consumption to 87.32 %, ensuring a high accuracy level (80.53 %) and a relatively execution time (48.47 ms).
Reynolds stress tensor and velocity measurements in technical flows by means of magnetic resonance velocimetry
Authors: John Kristine , Wüstenhagen Carolin , Schmidt Simon , Schmitter Sebastian , Bruschewski Martin , Grundmann Sven 
Published: February 2022

Abstract
Magnetic Resonance Velocimetry (MRV), an imaging method based on Magnetic Resonance Imaging (MRI), enables the measurement of flow parameters such as the velocity and the Reynolds Stress Tensor (RST) in complex structures without optical or physical access to the flow field. Several previous studies investigated the application of MRV velocity measurement in technical flows and obtained results that agreed well with reference data. However, only a few studies have investigated RST measurements using MRV beyond medical applications, and even though the qualitative results were promising, further work is required to establish this method. This study demonstrates the application of two-dimensional three-component (2D3C) velocity and six-component (2D6C) RST measurements in the flow field behind the sudden expansion of a scaled replica of the FDA benchmark nozzle. Particle Image Velocimetry (PIV) data accessible from an interlaboratory study was used for comparison. Furthermore, two different orientations of the imaging plane were measured to investigate the effect of the imaging orientation on the results. The measurement uncertainty of the mean axial velocity is 1.2 % related to the bulk velocity. The RST results agree well with the PIV data, but quantitative deviations occur in the areas where the influence of systematic errors was expected. Comparing different imaging orientations demonstrates that the sequence design affects the quantitative results of the measurement.
Electrochemical quantification of biomarker myeloperoxidase
Authors: Hoyo Javier , Bassegoda Arnau , Tzanov Tzanko 
Published: February 2022

Abstract
Point of care testing (PoCT) devices permit precise and rapid detection of disease-related biomarkers contributing to an early disease diagnosis and administration of an appropriate treatment. The enzyme myeloperoxidase (MPO) is a relevant biomarker for infection and inflammation events assessment; however its direct electrochemical quantification is hindered by the limited accessibility to the iron atom in its active center. Herein, such hindrance of the MPO biomolecule is overcome using the redox mediator 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The charge involved in the electrochemical reduction of the MPO-oxidized ABTS is correlated with the concentration of MPO. The use of ABTS allowed for the electrochemical assessment of a wide range of MPO concentrations (10–1000 nM) including those reported for wound infections, chronic obstructive pulmonary disease and early adverse cardiac events. The developed electroanalytical approach is rapid and inexpensive, and thus suitable for implementation in PoCT devices.
Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding system
Authors: Liu Jiaxue , Bao Xiaoli , Kolesnik Irina , Jia Boyan , Yu Zihan , Xing Caiyun , Huang Jiawen , Gu Tingting , Shao Xiaotong , Kletskov Alexey , Kritchenkov Andreii S. , Potkin Vladimir , Li Wenliang 
Published: February 2022

Abstract
To increase the in vivo stability of cationic gene carriers and avoid the adverse effects of their positive charge, we synthesized a new shielding material by conjugating low molecular weight polyethylene glycol (PEG) to a hyaluronic acid (HA) core. The HA-PEG conjugate assembled with the positively charged complex, forming a protective layer through electrostatic interactions. DNA/polyetherimide/HA-PEG (DNA/PEI/HA-PEG) nanoparticles had higher stability than both DNA/polyethyleneimine (DNA/PEI) and DNA/PEI/HA complexes. Furthermore, DNA/PEI/HA-PEG nanoparticles also showed a diminished nonspecific response toward serum proteins in vivo. The in vivo transfection efficiency was also enhanced by the low cytotoxicity and the improved stability; therefore, this material might be promising for use in gene delivery applications.
Altered endometrial oestrogen-responsiveness and recurrent reproductive failure
Authors: Al-Lamee Hannan , Ellison Amy , Drury Josephine , Hill Christopher J , Drakeley Andrew J , Hapangama Dharani K , Tempest Nicola 
Published: February 2022

Graphical abstract

Abstract
Recurrent reproductive failure (RRF) encompasses recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL). These highly prevalent, distressing conditions have many unanswered questions regarding aetiology and management. Oestrogen receptor beta (ERβ) is the predominant oestrogen receptor expressed in the vascular endothelium of the endometrium during the window of implantation (WOI). The establishment of normal endometrial receptivity is integrally associated with progesterone receptor (PR). Therefore, we aimed to investigate whether women with RRF have clinical, type-specific endometrial aberrations of ERβ, PR and Ki-67 expression during the WOI. Thirty-eight endometrial biopsies were collected; 29 RRF (10 RIF, 9 recurrent loss of early pregnancy (RLEP) and 10 recurrent fetal loss (RFL)) and 9 fertile controls (FC). Within RIF, RLEP and RFL groups, the perivascular compartment showed significantly lower levels of ERβ vs FC (P  = 0.02, P  = 0.03 and P  = 0.01, respectively). Vascular endothelium also displayed significantly lower levels of ERβ within RIF and RFL cohorts vs FC (P  = 0.03 and P  = 0.003). The expression of Ki-67 was significantly lower within vascular endothelium of all RRF; RIF (P  = 0.02), RLEP (P  = 0.02) and RFL (P  <0.01). PR was significantly reduced (P  <0.001) in the perivascular area of women with RIF. These findings provide novel insights into biological correlates of clinical subtypes of RRF. The endometrium of women with RRF display significantly altered levels of ERβ, PR and Ki-67 during the WOI, furthering our understanding of the defective endometrial phenotype of women suffering from RRF, with possible impaired glandular function, angiogenesis and decidualisation.

Lay summary
Recurrent reproductive failure (RRF) refers to a group of devastating conditions with many unanswered questions regarding their causes and treatment options. The lining of the womb, the endometrium, is primed and suitable for successful embryo implantation for a short time during the menstrual cycle; the window of implantation (WOI). Oestrogen is a key hormone that plays an important role in regulating the endometrium and its effects are exerted via two oestrogen receptor subtypes. Oestrogen receptor beta (ERβ) is the main oestrogen receptor present during the WOI. Progesterone receptor allows the other main hormone, progesterone, to influence the endometrial activity and Ki-67 reflects the proliferative activity of the cells within the endometrium. We investigated the expression of these markers in endometrial samples collected from women with RRF and proven fertility. We found that the endometrium of women with RRF has significantly lower levels of ERβ and Ki-67 during the WOI, possibly leading to unsuccessful pregnancies.
Lithuanian Education: Effective, Efficient, High Quality and Equitable?
Authors: Želvys Rimantas , Dukynaitė Rita , Stumbrienė Dovilė , Jakaitienė Audronė 
Published: February 2022

This paper discusses the problem of educational quality. The definition of quality is very broad; therefore, it is more convenient to use the term “educational effectiveness”. Effectiveness can be measured by choosing quantitative indicators that should reflect the level of student achievement as well as equity. It is also important to measure educational efficiency – the output in relation to the input. The current review presents key findings of research conducted in the domain of educational effectiveness and efficiency. The main conclusion of the research paper is that the currently existing mythology of educational quality should be replaced by evidence-based education policy.
Association of fluoroquinolone resistance and ESBL production in hypervirulent Klebsiella pneumoniae ST11 and ST893 in Iran
Authors: Talebzadeh Hamid , Mellali Hamid , Solgi Hamid 
Published: February 2022

Abstract
The spread of multidrug resistance in Klebsiella pneumoniae is a serious threat to the public health. In this study, the prevalence of fluoroquinolone resistance and virulence determinants among ESBL-producing K. pneumoniae isolates was investigated. A total of 50 third-generation cephalosporin resistant K. pneumoniae strains were collected from patients’ clinical cultures between September 1st, 2019 and February 30th, 2020. Clonal relatedness of clinical isolates was determined by multilocus sequence typing. All 50 isolates were multidrug-resistant (MDR) and carried at least one of the ESBL resistance determinants. The bla
CTX-M-15 gene was the major ESBL determinant found in K. pneumoniae (88%), followed by bla
SHV (86%) and bla
TEM (78%). PMQR was detected in 96% of the isolates and aac(6′)-Ib-cr was the most common (78%) as well as multiple mutations in gyrA (S83I, D87G) and parC (S80I) were found. Selected isolates were assigned to seven sequence types (STs) (ST11, ST893, ST147, ST16, ST377, ST13, and ST392). Overall, hypervirulent phenotypes were identiﬁed in 26 (52%) of the isolates. Among the 50 isolates, 28 (56%) were positive for ybt, 23 (46%) for rmpA, 17 (34%) for iroB, 15 (30%) for magA, 4 (8%) for alls and 3 (6%) for iucA genes. The K1 capsular type was the most prevalent (11/50; 22%) among isolates. The emergence of hypervirulent K. pneumoniae (hvKp) ST11 and ST893, which co-carried ESBL, PMQR determinants and different virulence genes has become a threat to the treatment of inpatients in the clinical setting.
Characteristics in the whole-genome sequence of Klebsiella pneumoniae ST147 from Turkey
Authors: Turumtay Halbay , Allam Mushal , Sandalli Aytül , Turumtay EMİNE AKYÜZ , Genç Hacer , Sandalli Cemal 
Published: February 2022

Abstract
The study aimed to analyze antibiotic resistance determinants in a carbapenem-resistant Klebsiella pneumoniae by whole-genome sequencing (WGS). K. pneumoniae was isolated from a urine sample and it was characterized by 16S rDNA sequencing in Turkey. This strain was named as Kpn Rize-53-TR. Antimicrobial susceptibility testing was performed for seventeen antibiotics by VITEK-2 and the result was confirmed by MIC. The whole genome of isolate was sequenced by Illumina and was analysed by bioinformatic tools for MLST, replicon types, and antimicrobial resistance genes. The whole genome data was submitted to NCBI. The isolate was found to be resistant to all tested β-lactam antibiotics and the highest MIC values were found for piperacillin, piperacillin/tazobactam (≥128). No resistance to colistin and moderate susceptibility to amikacin and tetracycline was observed. The isolate carried 12 resistance genes belonging to 10 resistance classes; ere(A), fosA, oqxB, cmlA1, aac(a)-IIa, bla
KPC-2, bla
TEM-1A, bla
SHV-67, bla
CTX-M-15, bla
OXA-1-2-9. Mutations were detected in gyrA (83Y) and parC (80I) genes. Clonal subtype of the isolate was ST147, and it had wzi420 and wzc38 alleles. Its serotype was O3/O3a. The bla
KPC-2 was firstly found in both ST147 clonal group in Turkey and in serotype O3/O3a in the world. By plasmid replicon typing, five plasmids IncFII(K), Col(BS512), IncR, IncFIA(HI1) and IncFIB(pQil) were determined in Kpn Rize-53-TR and bla
KPC-2 was located on IncFII(K) plasmid. The presence of bla
KPC-2 on the plasmid with other resistance genes accelerates its own spread together with other resistance genes.
Evaluation of Anti-Aflatoxin M1 effects of heat-killed cells of Saccharomyces cerevisiae in Brazilian commercial yogurts
Authors: Pires Rogério C. , Portinari Marina R. P. , Moraes Giulia Z. , Khaneghah Amin Mousavi , Gonçalves Bruna L. , Rosim Roice E. , Oliveira Carlos A. F. , Corassin Carlos H. 
Published: February 2022

This study aimed to assess the aflatoxin M1 (AFM1) levels in 72 samples of yogurt from eight processing plants in São Paulo, Brazil, and the ability of heat-killed cells of Saccharomyces cerevisiae (1010 yeast cells/g) to reduce AFM1 (0.5 µg/kg) in experimental yogurts (n = 3). Analyses were conducted by high performance liquid chromatography (HPLC). Only seven samples (9.8%) had AFM1 at a mean level of 0.071 ± 0.08 µg/kg. S. cerevisiae efficiently reduced (P < 0.05) the AFM1 levels in spiked yogurts, with a maximum reduction of 46% after 30 days of storage. Further studies should investigate potential effects of S. cerevisiae on the sensory properties of yogurts.
Curriculum Implementation Facilitating and Hindering Factors: The Philippines Context
Authors: Palestina Randy L , Pangan Agripina D , Ancho Inero V 
Published: February 2022

This study focuses on the perceptions of Filipino teachers and school administrators of the different facilitating and hindering factors in curriculum implementations based on their practices and the challenges they have experienced. Using descriptive research employing quantitative design, 324 respondents were surveyed using self-made questionnaires anchored on Ecological System Theory (EST) by Bronfenbrenner (1979). The respondents from the grade school, junior high school, and senior high school expressed their agreement on the identified factors that facilitate and hinder curriculum implementation in the microsystem and chronosystem, while respondents from the college expressed their strong agreement on the two levels under the mesosystem and exosystem. At the macrosystem level, all of the respondents strongly agreed on all the items, but the highest level of agreement was evident with the respondents from the senior high school. A comparison of one-way ANOVA results revealed the respondents’ level of agreement on the facilitating and hindering factors of curriculum implementation based on the five levels of EST. Scrutiny on the facilitating and hindering factors in the curriculum implementation processes provides a springboard for leaders in the education sector to align programs and policies anchored on experiences and practices in the classroom along with research-based inputs.