bluuebunny/crossref_metadata_2025_split huggingface dataset:
Induction of beige‐like adipocyte markers and functions in 3T3‐L1 cells by Clk1 and PKCβII inhibitory molecules
Authors: Patel Achintya , Dobbins Tradd , Kong Xiaoyuan , Patel Rehka , Carter Gay , Harding Linette , Sparks Robert P. , Patel Niketa A. , Cooper Denise R. 
Published: July 2022

AbstractExcessive dietary intake of fat results in its storage in white adipose tissue (WAT). Energy expenditure through lipid oxidation occurs in brown adipose tissue (BAT). Certain WAT depots can undergo a change termed beiging where markers that BAT express are induced. Little is known about signalling pathways inducing beiging. Here, inhibition of a signalling pathway regulating alternative pre‐mRNA splicing is involved in adipocyte beiging. Clk1/2/4 kinases regulate splicing by phosphorylating factors that process pre‐mRNA. Clk1 inhibition by TG003 results in beige‐like adipocytes highly expressing PGC1α and UCP1. SiRNA for Clk1, 2 and 4, demonstrated that Clk1 depletion increased UCP1 and PGC1α expression, whereas Clk2/4 siRNA did not. TG003‐treated adipocytes contained fewer lipid droplets, are smaller, and contain more mitochondria, resulting in proton leak increases. Additionally, inhibition of PKCβII activity, a splice variant regulated by Clk1, increased beiging. PGC1α is a substrate for both Clk1 and PKCβII kinases, and we surmised that inhibition of PGC1α phosphorylation resulted in beiging of adipocytes. We show that TG003 binds Clk1 more than Clk2/4 through direct binding, and PGC1α binds to Clk1 at a site close to TG003. Furthermore, we show that TG003 is highly specific for Clk1 across hundreds of kinases in our activity screen. Hence, Clk1 inhibition becomes a target for induction of beige adipocytes.
Deep brain stimulation for Parkinson's disease
Authors: Hariz Marwan , Blomstedt Patric 
Published: July 2022

AbstractParkinson's disease (PD) is a progressive neurodegenerative illness with both motor and nonmotor symptoms. Deep brain stimulation (DBS) is an established safe neurosurgical symptomatic therapy for eligible patients with advanced disease in whom medical treatment fails to provide adequate symptom control and good quality of life, or in whom dopaminergic medications induce severe side effects such as dyskinesias. DBS can be tailored to the patient's symptoms and targeted to various nodes along the basal ganglia–thalamus circuitry, which mediates the various symptoms of the illness; DBS in the thalamus is most efficient for tremors, and DBS in the pallidum most efficient for rigidity and dyskinesias, whereas DBS in the subthalamic nucleus (STN) can treat both tremors, akinesia, rigidity and dyskinesias, and allows for decrease in doses of medications even in patients with advanced stages of the disease, which makes it the preferred target for DBS. However, DBS in the STN assumes that the patient is not too old, with no cognitive decline or relevant depression, and does not exhibit severe and medically resistant axial symptoms such as balance and gait disturbances, and falls. Dysarthria is the most common side effect of DBS, regardless of the brain target. DBS has a long‐lasting effect on appendicular symptoms, but with progression of disease, nondopaminergic axial features become less responsive to DBS. DBS for PD is highly specialised; to enable adequate selection and follow‐up of patients, DBS requires dedicated multidisciplinary teams of movement disorder neurologists, functional neurosurgeons, specialised DBS nurses and neuropsychologists.
Evaluation of public health nurse‐led health counseling programs for the prevention of cardiovascular diseases in Amagasaki City, Japan
Authors: Noguchi Midori 
Published: July 2022

AbstractObjectiveWe evaluated whether Amagasaki health counseling programs led by public health nurses (PHNs) are contribute to improving cardiovascular disease (CVD) risk factors and CVD mortality among adult residents.Design and sampleThis cross‐sectional study used a before‐and‐after comparative design. We evaluated data from health checkups and mortality before and after Amagasaki health promotion programs.MeasurementsPopulation levels of CVD risk factors body mass index (BMI), waist circumference, systolic and diastolic blood pressure, fasting blood glucose, hemoglobin A1c, low‐density lipoprotein (LDL) cholesterol, high‐density lipoprotein (HDL) cholesterol, Triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid, γ‐glut amyl trans peptidase (γ‐GTP), serum creatinine, estimated glomerular filtration rate (eGFR) were compared between 2008 and 2012 when the intervention with the Amagasaki health counseling program started from 2008. In addition, standardized mortality ratio (SMR) of acute myocardial infarction (AMI) were compared between 2003–2007 and 2008–2012.ResultsMean value of most CVD risk factors improved and the SMR for AMI declined, which could be attributable in part to the effort of intensive health education and counseling.
Mutational landscape of primary breast angiosarcoma with repeated resection and recurrence over a 15‐year period: A case report
Authors: Teruyama Fumiya , Kuno Akihiro , Murata Yoshihiko , Nakagawa Tomoki , Shiba‐Ishii Aya , Yuguchi Shu , Noguchi Masayuki 
Published: July 2022

AbstractAngiosarcoma is a rare malignant tumor derived from vascular endothelial cells and has a poor prognosis. We have experienced a case of multiple breast angiosarcoma for which multiple resections had been performed during the course of its progression over a period of more than 15 years, allowing comprehensive genetic mutation analysis. Somatic mutations in several cancer‐related genes were detected, but no previously reported driver gene mutations of angiosarcoma were evident. Several germline mutations associated with malignancy, such as single nucleotide polymorphisms in Fibroblast Growth Factor Receptor 4 (FGFR4) (p.Gly388Arg, rs351855), Kinase Insert Domain Receptor (KDR) (Gln472His, rs1870377) and tumor protein p53 (TP53) (p.Pro72Arg, rs1042522) were detected. Common signatures and genetic mutations were scarce in the tumor samples subjected to genetic mutational analysis. These findings suggested that this case was very probably a multiprimary angiosarcoma.
less-than-human academia
Authors: Kallio Kirsi Pauliina , Häkli Jouni , Riding James 
Published: July 2022

The editorial discusses the impacts of the global pandemic to human agency, through the authors self-reflection of academic work during the past two years accompanied two philosophical perspectives. First, we return to Chris Philo’s (2017) conception of ‘less-than-human geographies’, and secondly, to Helmuth Plessner’s (2019) conception of the sociality of human embodiment. What these perspectives have helped us to see is that people, including academic communities, need embodied encounters to fully experience ourselves and others as humans – humans whose exceptionality is not about superiority over non-human nature but eccentricity that offers us possibilities to avoid inhumanity. In conclusion we note the value of embodied encounters as a constitutive aspec of humane social life, particularly in the current times of war in Europe.
Identification Method for Material Constants in Johnson–Cook Model Associated With Strain-Rate and Temperature Sensitivities Using Ball Impact and Indentation Tests
Authors: Ito Kiyohiro 
Published: July 2022

Abstract
The Johnson–Cook (JC) flow stress model can simply express the strain-rate and temperature dependencies of the flow stress. We previously proposed a concept of a simple identification method for material constants in the JC model associated with the strain-rate and temperature sensitivities (JC parameters C and m) at high-strain rates. However, several issues still exist in our method for practical use. In this study, we modified our method to identify both the JC parameters C and m. The fundamental concept was formulated based on the requirement that the normalized indentation depths obtained from the ball impact and instrumented ball indentation tests are equal to each other. In addition, the conversion factor was derived to transfer from the impact velocity to the strain rate. The ball indentation and impact analyses based on a finite element method were conducted to verify the established method. The analysis results obtained under idealized conditions demonstrated that C and m can be accurately identified using the established method, even at exceeding 104 s−1. Changes in the radius of the impactor are more effective in obtaining different strain rates than changes in the impact velocity because the identification accuracy can be ensured at lower than 200 m/s.
Broadband Electromechanical Diode: Acoustic Non-Reciprocity in Weakly Nonlinear Metamaterials With Electromechanical Resonators
Authors: Bukhari Mohammad , Barry Oumar 
Published: July 2022

Abstract
Recent attention has been given to acoustic non-reciprocity in metamaterials with nonlinearity. However, the study of asymmetric wave propagation has been limited to mechanical diodes only. Prior works on electromechanical rectifiers or diodes using passive mechanisms are rare in the literature. This problem is investigated here by analytically and numerically studying a combination of nonlinear and linear metamaterials coupled with electromechanical resonators. The nonlinearity of the system stems from the chain in one case and from the electromechanical resonator in another. The method of multiple scales is used to obtain analytical expressions for the dispersion curves. Numerical examples show potential for wider operation range of electromechanical diode, considerable harvested power, and significant frequency shift. The observed frequency shift is demonstrated using spectro-spatial analyses and it is used to construct an electromechanical diode to guide the wave to propagate in one direction only. This only allows signal sensing for waves propagating in one direction and rejects signals in any other direction. The performance of this electromechanical diode is evaluated using the transmission ratio and the asymmetric ratio for a transient input signal. Design guidelines are provided to obtain the best electromechanical diode performance. The presented analyses show high asymmetry ratio for directional-biased wave propagation in the medium-wavelength limit for the case of nonlinear chain. Indeed, the present asymmetric and transmission ratios are higher than those reported in the literature for a mechanical diode. The operation frequencies can also be broadened to the long-wavelength limit frequencies using the resonator nonlinearity.
Hungry bone syndrome like presentation following single-dose denosumab for hypercalcaemia secondary to sarcoidosis with IgA nephropathy
Authors: Nachankar Amit , Katyal Amit , Bansal Naresh , Bishnoi Alka 
Published: July 2022

A woman in her mid-50s with IgA nephropathy, sarcoidosis and steroid-induced diabetes mellitus presented with generalised paraesthesia and spontaneous tetany. She had received denosumab 60 mg subcutaneously 8 weeks previously for parathyroid hormone independent hypercalcaemia.At admission, she had severe hypocalcaemia (5 mg/dL), hypophosphataemia (1.9 mg/dL), hypomagnesaemia (1.4 mg/dL) and elevated serum creatinine (1.48 mg/dL) with prolonged QTc (corrected QT interval) on electrocardiograph. She initially received intravenous calcium and magnesium followed by oral calcium carbonate and calcitriol. Her prednisolone dose was tapered to 5 mg/day. Evaluation showed secondary hyperparathyroidism (1474 pg/mL) and elevated 1,25-dihydroxy vitamin D (195 pg/mL). After 1 week of oral calcium carbonate (3000 mg/day) and calcitriol (1.5 µg/day), she achieved normocalcaemia (8.1 mg/dL).To conclude, denosumab for hypercalcaemia with renal insufficiency causes prolonged severe symptomatic hypocalcaemia and hypophosphataemia mimicking hungry bone syndrome. It is important to periodically monitor for hypocalcaemia after denosumab.
Effects of biophilic virtual reality on cognitive function of patients undergoing laparoscopic surgery: study protocol for a sham randomised controlled trial
Authors: Zhu Binbin , Zhou Yanling , Weng Qiuyan , Luo Wenjun , He Xiuchao , Jin Lufei , Wang Qinwen , Gao Bin , Harb Ahmad Khaled Ahmad , Wang Jianhua 
Published: July 2022

IntroductionVirtual reality (VR) is already being used for cognitive or emotional rehabilitation. However, its role in postoperative cognitive dysfunction (POCD) has not been fully recognised. Due to the lack of an effective postoperative follow-up system, the incidence of POCD in China is not clear, and although many drugs have been proposed to improve POCD in the animal study, their clinical applications are limited, while VR provides an innovative method to provide non-pharmacological management.Methods and designThis is a single-centre, randomised, double-blind, sham-controlled clinical trial. In this study, 600 patients over 55 years old undergoing laparoscopic surgery will be recruited. Participants will be randomly assigned to receive biophilic VR or sham VR (1:1 ratio), all patients have 20 min of exposure per day during the hospital stay. The primary outcome is the impact of VR on the incidence of POCD. Secondary outcomes include perioperative anxiety and instrumental activities of daily living. Changes in the performance of the neurocognitive batteries are measured by a local resident doctor. Serum samples will be collected on the day before surgery and 7 days after surgery.Ethics and disseminationThis trial has ethical approval from the Medical Ethics Committee of the Affiliated Hospital of Medical School of Ningbo University (KY20210302). The study is sponsored by Ningbo University and Ningbo Science and Technology Bureau. Contact: Dr. Mao Haijiao, Chair of the hospital medical Ethics committee (ndfylunli@126.com). Trial results will be submitted for publication in peer-reviewed journals, patient recruitment began in April 2021. Written informed consent is obtained for all participants. All information acquired will be disseminated via national or international conferences and published in peer-reviewed journals.Trial registration numberChiCTR2000040919.
Daily versus three-times-weekly azithromycin in Chinese patients with non-cystic fibrosis bronchiectasis: protocol for a prospective, open-label and randomised controlled trial
Authors: Mao Yanxiong , Chen Lan , He Ting , Li Jing , Zou Aiping , Li Feng , Chen Fei , Fan Bo , Ni Weihao , Xiao Wei , You Huimin , Fu Wenjiang 
Published: July 2022

IntroductionNon-cystic fibrosis bronchiectasis (NCFB) brought a heavy healthcare burden worldwide. Macrolide maintenance therapy was proved to be helpful in reducing exacerbation of NCFB. However, the optimal dosing regimens of macrolides have not been determined, and its efficacy in Chinese NCFB population has not been validated. This protocol describes a head-to-head clinical trial designed to compare the efficacy of two dosing regimens of azithromycin in Chinese NCFB population.Methods and analysisThis prospective, open-label and randomised controlled trial will be conducted in the First People’s Hospital of Jiashan, China. Eligible patients with high-resolution CT defined NCFB will be randomly divided into three groups, which will receive either 250 mg daily azithromycin, or 500 mg three-times-weekly azithromycin or no treatment for 6 months. They will be followed up for another 6 months without treatment. The primary outcome is the mean rate of protocol-defined pulmonary exacerbation at 6 months.Ethics and disseminationEthical approval was obtained from the First People’s Hospital of Jiashan Ethics Committee. The findings will be disseminated in peer-reviewed publications.Trial registration numberChiCTR2100052906.
Opinions on hypertension care and therapy adherence at the healthcare provider and healthcare system level: a qualitative study in the Hague, Netherlands
Authors: van Grondelle Saskia E , van Bruggen Sytske , Meijer Judith , van Duin Erik , Bots Michiel L , Rutten Guy , Vos Hedwig M M , Numans Mattijs E , Vos Rimke C 
Published: July 2022

ObjectivesHypertension is a common cause of cardiovascular morbidity and mortality. Although hypertension can be effectively controlled by blood pressure-lowering drugs, uncontrolled blood pressure is common despite use of these medications. One explanation is therapy non-adherence. Therapy non-adherence can be addressed at the individual level, the level of the healthcare provider and at the healthcare system level. Since the latter two levels are often overlooked, we wished to explore facilitators and barriers on each of these levels in relation to hypertension care for people with hypertension, with a specific focus on therapy adherence.DesignQualitative study using focus groups of healthcare providers. Data were analysed using the theoretical domains framework (TDF) and the behaviour change wheel.Setting and participantsParticipants were from a highly urbanised city environment (the Hague, Netherlands), and included nine primary care physicians, six practice nurses and five secondary care physicians involved in hypertension care.ResultsNine domains on the TDF were found to be relevant at the healthcare provider level (‘knowledge’, ‘physical, cognitive and interpersonal skills’, ‘memory, attention and decision processes’, ‘professional, social role and identity’, ‘optimism’, ‘beliefs about consequences’, ‘intention’, ‘emotion’ and ‘social influences’) and two domains (‘resources’ and ‘goals’) were found to be relevant at the system level. Facilitators for these domains were good interpersonal skills, paying attention to behavioural factors such as medication use, and the belief that treatment improves health outcomes. Barriers were related to time, interdisciplinary collaboration, technical and financial issues, availability of blood pressure devices and education of people with hypertension.ConclusionsThis study highlighted a need for better collaboration between primary and secondary care, for more team-based care including pharmacists and social workers, tools to improve interpersonal skills and more time for patient–healthcare provider communication.
Primary care experiences of providing mental healthcare for children in the USA during the COVID-19 pandemic: a qualitative study
Authors: Garbutt Jane , Dodd Sherry , Rook Shannon , Ericson Lauren , Sterkel Randall , Plax Katie 
Published: July 2022

BackgroundThe increased demand for mental healthcare associated with the COVID-19 pandemic adds to the already high unmet mental health needs among paediatric patients, resulting in a declared ‘mental health emergency’. The study objective was to describe paediatric primary care providers (PCPs’) experience of meeting their patients’ needs for mental healthcare during the pandemic and to identify opportunities to optimise care.Methods19 paediatricians and 2 nurse practitioners completed a 30 min video interview in May 2021. Interviews were recorded and transcribed, and transcripts were analysed using consensual qualitative research methods.ResultsParticipants reported marked increases in patient mental health needs during the COVID-19 pandemic. These included new diagnoses of anxiety and depression and increased treatment needs for patients with a prior mental health diagnosis. They identified that the mental health needs of their patients were greater, more severe and more challenging to manage with the resources currently available. While they were frustrated with the lack of communication and support from their mental health colleagues, and felt isolated and overwhelmed, they approached the increased demand for mental healthcare with a growth mindset. This outlook included providing care, seeking help to improve their skills and engaging with local resources such as the Child Psychiatry Access Project (CPAP).ConclusionsOur findings suggest that urgent action is needed to better support paediatric PCPs to provide mental healthcare in our community. Providers identified an ongoing need for timely access to and effective communication with mental health providers to guide care in the medical home. This need could be addressed immediately by providing training for new and experienced clinicians, expanding the scope of CPAP programmes to include patient assessment and supporting implementation of integrated behavioural health programmes into the medical home.
Evaluation of intervertebral body implant performance using active surveillance of electronic health records
Authors: Frankenberger Edward A , Resnic Frederic S , Ssemaganda Henry , Robbins Susan , Dunbar Melissa R , Coplan Paul , Zhang Shumin , Bruno Cortney , Maltenfort Mitchell , Benedetti Jillian B , Matheny Michael E , Ghogawala Zoher 
Published: July 2022

ObjectivesTo assess the feasibility of using electronic health record (EHR) derived clinical data within an active surveillance setting to evaluate the safety of a novel intervertebral body implant (IVBI) stabilization device.DesignRetrospective, longitudinal observational cohort study comparing clinical outcomes for patients seen through 1 year following spinal fusion surgery.SettingLahey Health network, which includes academic tertiary hospitals, outpatient clinics, and independent provider offices in the New England region of the USA.ParticipantsAll spine surgery patients aged 18 or older who underwent thoracic or lumbar spinal arthrodesis surgeries were included.Main outcome measuresThe clinical outcomes of patients treated with the CONCORDE Bullet (CB) interbody spine system (DePuy) between April 2015 and December 2018 were compared with those patients receiving alternative spine stabilization interbody device implants. The primary endpoint was reoperation rate at 1 year, with secondary endpoints including the requirement for blood transfusion during index hospitalization, 1 year rate of any cause hospitalization, 1 year rate of surgical site infection, and mortality at 1 year.ResultsAmong the 606 patients undergoing thoracic or lumbar spinal fusion surgery during the study period, 136 received only the CB. In comparison with patients who did not receive the CB, no significant differences were found in the rate of reoperation at 1 year or the rates of secondary safety outcomes.ConclusionsData derived from the EHR can be successfully leveraged to assess the safety of IVBI devices, in this case demonstrating no significant differences in the rates of risk-adjusted safety endpoints between patients undergoing spinal surgery with the CB as compared with alternative spinal implants.