bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of the prognostic role and mutational characteristics of m6A-related genes in lung adenocacinoma
Authors: Chen Jing-Hua , Chu Xiang-Peng , Chen Zi-Hao , Chen Bi-Jun , Jiang Shao-Kun , Rosell Rafael , Zhong Wen-Zhao 
Published: October 2022

Abstract
Background
Lung adenocarcinoma (LUAD) is an invasive disease, which originates from small airway epithelial cells or alveolar type II cells. N6-methyladenosine (M6A) RNA methylation is involved in diverse biological processes. Studies have shown that m6A RNA methylation abnormalities play an important role in the pathogenesis of many human diseases, including cancer. The present study explores the prognostic role and mutational characteristics of m6A-related genes in LUAD.
Methods
RNA-seq and somatic mutation data of Genomic Data Commons and The Cancer Genome Atlas (GDC TCGA)-LUAD were downloaded from UCSC Xena for comprehensive analysis. M6A-related genes were selected from the content of RNA m6A modification in cancer. M6A genes were further screened by comparing the significance and survival of m6A genes between the normal group and the tumor group and analyzing the relationship between m6A genes and LUAD patients.
Results
GDC LUAD data were downloaded from UCSC Xena public database, and the gene expression data of 19 m6A methylation regulators in LUAD were extracted for differential expression levels. Then, the mutation of m6A genes was analyzed based on the single nucleotide polymorphism data of UCSC Xena. To further confirm the prognostic genes, univariate Cox regression and the Kaplan Meier method were used to determine the relationship between their expression level and the overall survival time in the TCGA database. Finally, HNRNPC, IGF2BP1, and IGF2BP3 genes were established for subsequent analysis. Correlation analyses showed that the three genes were correlated with clinical features and immune cell infiltration. Enrichment analysis revealed that HNRNPC was mainly enriched in ribonucleoprotein complex biogenesis, IGF2BP1 in the mitotic cell cycle checkpoint, and IGF2BP3 in the nuclear division pathway.
Conclusions
Our study identified novel immune-related prognostic markers of LUAD. Moreover, the potential mechanisms of prognostic markers in regulating the etiology of LUAD were investigated. These findings enrich our understanding of the relationships between immune and LUAD and may provide new insights into the treatment of LUAD patients.
Greening the financial regulation:Case of credit limit optimization for renewable energy
Authors: HIMRI Hicham , SAHNOUN Kacem , BAZZI Mehdi , Alaoui AbdelKader Ouatik El 
Published: October 2022

Abstract
The sense of urgency surrounding climate change is increasing over time, pushing the banking system to effectively align sustainable financial rules and project financing with the Paris Agreement’s Goals. Central banking lies on a set of instruments that address financial market functioning and financial stability at both the micro- and macroprudential levels. These instruments range from awareness to regulation and supervision. Our contribution consists in implementing one of these instruments, namely, credit limits, by employing mathematical modeling to optimize fund allocation in favor of the renewable energy sector, which is regarded as one of the least polluting. Our numerical application for a simulated bank portfolio has assisted in channeling a higher proportion of loans into renewable energy at the expense of other sectors that pose a high financial and environmental risk. Our findings have policy implications for both investors and policymakers.
Inter-pregnancy Interval Following Missed Abortion and the Risk for Preterm Birth
Authors: Bachar Gal , Abboud Yousef , Farago Naama , Justman Naphtali , Khatib Nizar , Zipori Yaniv , Weiner Zeev , Vitner Dana 
Published: October 2022

Abstract
Propose: Short inter-pregnancy interval (IPI), less than 18 months following live birth, has been associated with adverse pregnancy outcome. Our objective was to evaluate whether a short IPI following a medically treated missed abortion (MA) poses similar perinatal risks in a subsequent pregnancy.
Study Design: We performed a retrospective cohort study on women with MA, treated with Misoprostol (prostaglandin E1), and with a documented subsequent live pregnancy, at a single tertiary medical center (2010-2022). We excluded women who had surgical evacuation following failure of medical treatment. Women were allocated into two groups: IPI ≤ 18 months and IPI > 18 months. The primary outcome was the risk for a spontaneous preterm birth before 37 weeks of gestation in the consecutive pregnancy. Secondary outcomes included additional maternal and neonatal adverse outcome.
Results: We included 1,110 pregnancies: 430 (38.74%) women with IPI < 18 months and 680 (61.26%) women with IPI > 18 months. The characteristics of the two groups were not significantly different (Table 1), including Misoprostol number of doses.
The rate of spontaneous preterm birth < 37 and < 34 weeks’ gestation were significantly higher among women with a shorter IPI (16.28% vs. 7.06% and 6.74% vs. 5.0%, respectively, p<0.05). These women also had a higher risk for cesarean delivery (31.63% vs. 23.34%, p=0.005) and postpartum hemorrhage (PPH) (4.42% vs. 2.06%, p=0.029) compared to women with IPI > 18 months. No other significant differences were noted.
Conclusion: Short IPI (≤ 18 months) following a medical treatment for MA is associated with an increased risk of preterm birth, cesarean delivery, and PPH. Our findings may be particularly important for counseling women following missed abortion who desire another pregnancy.
Neurobehavioral and neurochemical basis of compulsive behavior: A 7T magnetic resonance spectroscopy study in humans
Authors: Biria Marjan , Banca Paula , Healy Mairead , Keser Engin , Sawiak Stephen , de Souza Ana Maria Frota Lisbôa Pereira , Marzuki Aleya , Sule Akeem , Ersche Karen , Robbins Trevor 
Published: October 2022

Abstract

There has been relatively little analysis of possible neurochemical correlates of compulsive behavior to illuminate its underlying neural mechanisms. We utilised 7-Tesla proton magnetic resonance spectroscopy (1H-MRS) to assess the balance of excitatory and inhibitory neurotransmission by measuring glutamate and GABA levels in anterior cingulate cortex and supplementary motor area (SMA) of healthy volunteers and patients with Obsessive-Compulsive Disorder (OCD). Within the SMA, trait and clinical measures of compulsive behavior were related to glutamate levels, whereas a behavioral index of habitual control correlated with the glutamate:GABA ratio. OCD patients additionally exhibited elevated glutamate levels and glutamate:GABA ratios in anterior cingulate cortex which also correlated with habitual control. This study highlights important underlying relationships between SMA mechanisms of habitual control relevant to compulsive behavior, common to the healthy sub-clinical and OCD populations. The results also demonstrate additional involvement of anterior cingulate in the balance between goal-directed and habitual responding in OCD.
Management Of Foreign Bodies In The Gastrointestinal Tract: A Retrospective Study Of 24 Cases
Authors: Guo Zhuang , Zhang Taotao , Xue Jiaming , Yang Huiliang , Zhang Xiangwen 
Published: October 2022

Abstract
Background: Foreign body ingestion is frequently encountered in clinical practice. However, few studies have focused on gastrointestinal foreign body ingestion. This study aims to analyze the location of gastrointestinal foreign body ingestion and treatment measures to report our experience in its management.
Methods: Data were collected from all patients with foreign body ingestion accepting management in our center from September 2016 to July 2022. The demographic data, type, location, clinical features, and management were reviewed and analyzed retrospectively.
Results: A total of 24 cases with foreign body ingestion underwent management. Jujube pit ingestion was the most common (16 cases). Most of the ingested foreign bodies were located in the intestine (21 cases), especially in the small intestine (7 cases). One case in the duodenum and one in the rectum underwent conservative management, 3 cases in the stomach and 2 cases in the intestine underwent endoscopic treatment, and 17 cases in the intestine underwent surgical intervention.
Conclusions: Ingested foreign body is usually secondary to unconscious accidental ingestion and is frequently caused by dietary habits. Foreign body ingestion in the GI tract usually happens in the small intestine with perforation-peritonitis. Once foreign body ingestion is diagnosed, it must be decided on therapeutic regimens based on the location of foreign body.
Effects of morality and reputation on risk-taking behaviors on behalf of robots
Authors: Chen Na , Zhai Yanan , Liu Xiaoyu , Hu Xueyan 
Published: October 2022

Abstract
The relationship between robots and humans is becoming increasingly intimate and will continue to become an integral part of life, with robots working together with human beings. In teamwork, individual decision making for others is influenced by morality and reputation. However, the effect of individuals’ decision making for others in human-robot collaborative teams remains unclear, and individuals may have different degrees of consideration for morality and reputation when making decisions for robots or humans. This research conducted three experiments based on Gneezy and Potters' investment decision-making experimental paradigm and compared the influence mechanisms of morality and reputation on decision making for others in human teams and human-robot teams. Experiment 1 verifies that when the individual decision-making objects were robots and human beings, the decision making for others was different, When the decision object is a robot, the risk preference of the decision making for others’ behavior is higher .Experiment 2 verifies that morality and reputation are the influencing factors of the differences in the decision making for others. Experiment 3 proposes the mechanism of the difference between the decision making for others in a human team and a human-robot team. That is, in a human-robot team, reputation is a mediating variable of the influence of morality on the decision making for others, while in a human team, there is no such mediating effect. The research results show that people's decision making for others is influenced by their cooperation partner. When the cooperation partner is a robot, the risk preference of the decision making for others is higher because of the low constraint of social morality. However, if a good reputation is maintained for morality considerations, then the risk preference of the decision making for others will be reduced.
Effects of environmental exposures on the barely visible impact damage of flax fibre reinforced biocomposite and its glass fibre hybrids
Authors: Ahamed Muneer Ahmed Musthaq Musthaq , Dhakal Hom Nath , Zhang Zhongyi , Barouni Antigoni , Lupton Colin , Zahari Rizal Bin , Shibli Abdullah Al , Babaa Saleh Elkelani , Jiang Chulin 
Published: October 2022

Abstract
Exposure of biocomposites to various environmental conditions is a concern when used for structural and semi-structural outdoor applications. This study investigates the effect of different environmental exposures on the low-velocity impact damage behaviour of flax fibre reinforced epoxy composite and its glass/flax hybrids. Flax and flax/glass hybridised epoxy laminates were fabricated using the vacuum infusion technique. A drop-weight low-velocity impact tests were performed on composite laminates at 5 J of incident energy with sub-zero temperatures, i.e., at (-10°C and − 20°C) and room temperature (R.T.). In order to evaluate the induced damage at different temperatures, micro-computed tomography (µ-CT) and visual inspection techniques were employed. The experimental results show that at a sub-zero temperature of -20°C, the flax and hybrid glass/flax composites showed more brittle damage behaviour than at room temperature. Furthermore, these experimental results have shown that alternating hybrid glass/flax laminates exhibited noticeable transverse cracks at R.T. and − 10°C., with higher absorbed energy and maximum impact load-carrying ability arising from positive hybrid effects. Also, it is evidenced from the results that the hybrid approach can be a viable strategy for achieving improved impact performance of natural plant fibre reinforced composites (NPFRCs) when exposed to different environmental conditions.
Size effect in PbS Quantum Dots Luminescence
Authors: Grevtseva Irina , Chevychelova Tamara , Ovchinnikov Oleg , Smirnov Mikhail , Kondratenko Tamara , Khokhlov Vladimir , Zvyagin Andrey , Astashkina Marina 
Published: October 2022

Abstract
This paper presents new regularities of the size effect in IR luminescence of semiconductor colloidal PbS quantum dots, passivated with thioglycolic acid molecules (PbS/TGA QDs). It was found that decrease in the average PbS/TGA QDs size of the sample from 4.9 nm to 2.6 nm produces the luminescence peak shifts from 1350 nm to 865 nm. At that time, in the excitation spectrum the peak due to exciton absorption shifts from 1235 nm to 707 nm. The Stokes shift increases from 0.086 to 0.32 eV. The study of time-resolved luminescence showed that the luminescence decay is complex non-exponential it, that is determined by the statistical distribution of luminescence quenchers in PbS/TGA QDs over the ensemble. The complex of the obtained experimental results made it possible to conclude that the observed luminescence is determined by the radiative annihilation of exciton in PbS/TGA QDs.
Characterization and genome analysis of the novel phage VS1 infecting the pathogenic bacterium Vibrio splendidus of Apostichopus japonicus
Authors: Duan Xuemei , Jiang Liming , Guo Ming , Li Chenghua 
Published: October 2022

Abstract
Vibrio splendidus is one of the common pathogens in the ocean and infects Apostichopus japonicus, Atlantic salmon and Crassostrea gigas, leading to a variety of diseases. In this study, a virulent phage, VS1, which infects V. splendidus, was isolated from aquaculture ponds in Dalian, China. Its genome annotation and characterization were detected. The complete genome of phage VS1 consists of a linear double-stranded DNA that is 248,270 bp in length with an average G + C content of 42.5% and 144 putative protein-coding genes, and 116 genes have known functions. There are 4 tail fiber genes in the positive and negative strands of the phage VS1 genome. The domains and crystal structure of the phage VS1 tail fibers were obtained from the Protein Data Bank and SMART (http://smart.embl.de) database. The bacteriostatic effect of phage VS1 on V. splendidus culture was 93.5 times. Comparative genomic and phylogenetic analyses indicate that phage VS1 is a novel phage. This study provides the genome characterization of the novel phage VS1 that infects V. splendidus.
Characterization and establishment of an immortalized rabbit ovary granulosa cell line for reproductive biology experiments
Authors: Bao Zhiyuan , Chen Yang , Li Jiali , Zhang Xiyu , Dai Yingying , Wang Fan , Cai Jiawei , Ren Yuyao , Zhao Bohao , Wu Xinsheng 
Published: October 2022

Abstract
Granulosa cells (GCs) are the key components of ovarian follicles for regulating oocyte maturation. communicating with oocytes through complex gap junctions and regulate the growth and development of oocytes. In addition to being considered to have great potential for human therapeutic model development and livestock breeding. Here, we transformed lentivirus-mediated simian virus 40 Large T (SV40LT) into primary rabbit granulosa cells (Pri RGCs) to establish an immortalized cell line. Morphologically, the immortalized RGCs (Im RGCs) were indistinguishable from the Pri RGCs, cell structure was intact following H&E staining. No significant difference in cell proliferation viability and growth between Im RGCs and Pri RGCs. Based on GCs-specific markers, the expression of FSHR, StAR, CYP11A1 and CYP17A1 were identified by PCR, immunofluorescence, and western blot analysis. ELISA shows that ImRGCs can synthesize steroid hormones normally. Karyotype analysis showed that the number of chromosomes remained normal during the process of infinite passage, soft-agar cloning experiment and nude mice tumorigenic experiment Indicates that the ImRGCs were not malignantly transformed. In conclusion, the immortalized granulosa cell line of rabbit follicle was established and can be used as a tool cell for future studies on biological research, animal husbandry and female reproductive disease.
Small Protein NBASP Encoded by lncRNA FAM201A Decreases Neuroblastoma Progression via FABP5
Authors: Ye Mujie , Gao Runnan , Chen Shiyu , Bai Jianan , Chen Jinhao , Lu Feiyu , Gu Danyang , Shi Xiaoting , Yu Ping , Tang Qiyun , Dong Kuiran 
Published: October 2022

Abstract
Increasing evidence indicates that long non-coding RNA (lncRNA) is one of the most important RNA regulators in the pathogenesis of neuroblastoma (NB). However, limited studies have addressed the possibility that lncRNAs possess the ability of encoding functional peptides, which could affect the initiation and development of tumors by participating in signal pathway transduction, immune regulation, and tumor
metabolism. Here, we found that downregulation of lncRNA FAM201A was associated with NB malignant progression and a poor prognosis. A variety of gain- and loss-of-function studies elucidated the anti-tumor effects of lncFAM201A on the
regulation of growth and metastasis of NB cells. Surprisingly, combined with database predictions and experimental verifications, we found that significantly down-regulating lncRNA FAM201A in NB identified the ability to encode the
tumor-suppressing micropeptide, NBASP, which negatively regulated the expression of FABP5. Mechanistically, our transcriptomics analyses confirmed that the regulatory role between NBASP and FABP5 was mediated by activation of the ERK
pathway. In conclusion, our findings revealed that NBASP encoded by lncRNA FAM201A played a tumor-suppressor role in NB carcinogenesis via down-regulating FABP5 to inactivate the ERK pathway. These results extended our understanding of the relationship of lncRNA-encoded functional peptides and plasticity of tumor progression.
Necessity of pathological examination of reflux esophagitis
Authors: Chen Qianqian , Linghu Enqiang , Zhu Wenya , Niu Shaoli , Li Yi , Li Jia 
Published: October 2022

Abstract
Background: Routine biopsies are not recommended in the guidelines for RE patients, whereas upper gastrointestinal endoscopy combined with histopathological examination is necessary for RE patients with high-risk factors.
Methods: The medical records of 613 patients with RE who had undergone upper gastrointestinal endoscopy and biopsy for histopathological examination were collected. Relevant and risk factors for pathological outcomes were analyzed.
Result: The proportions of middle-aged and male participants were higher. The pathological outcome was related to age and endoscopic findings, but not sex. Age is a risk factor for intestinal metaplasia and is associated with esophagitis severity. Esophageal mucosa status, white moss, and hemostasis with adrenaline are relevant with LIN,only mucosal status is a risk factor. LA Grade is a significant risk factor for HIN. Mucosa status and white moss are both risk factors for erosive ulcer and pathological follow-up necessity.
Conclusion: Biopsy and pathological follow-up of cases with risk factors, especially endoscopic mucosal abnormalities, help screen for precancerous lesions and reduce misdiagnosis.
Application of feed-forward neural network approaches to ApplicationApplication of feed-forward neural network approaches to radiomics-based survival analysis in glioma patients radiomics-based survival analysis in glioma patients
Authors: Remigio Adrian Sabariaga , Franich Rick , Panettieri Vanessa 
Published: October 2022

Abstract
Radiomic features extracted from MR images, along with other clinical covariates, have been shown to facilitate glioma patient prognostication via survival analysis. In this study, we apply the DeepSurv and neural network-parameterized probability mass function (PMF-NN) models to the survival analysis of glioma patients using the age and extracted radiomic features from multiparametric MRIs in the Brain Tumor Segmentation Challenge 2018 (BraTS 2018) dataset. First order and texture features were calculated for the whole tumor and peritumoral brain zone (PBZ) regions in both T1-weighted contrast-enhanced (T1-wce) and T2-weighted fluid attenuated inversion recovery (T2-FLAIR) images. To the best of our knowledge, this work is the first to apply the DeepSurv and PMF-NN models to radiomics-based survival analysis of glioma patients. Whole tumor shape features were also calculated from the delineated masks of the whole tumor. Pearson correlation analysis showed age, 4 first-order, 10 shape, and 17 texture features are correlated with the overall survival of glioma patients. These correlated features were used as input for the developed DeepSurv and PMF-NN models. The best performing DeepSurv model in our study obtained a 1050-day integrated Brier score (IBS) and C-index of 0.122 and 0.670, respectively. For the best performing PMF-NN in terms of IBS, a 1050-day IBS of 0.125 and a C-index of 0.632 were obtained. Thus, our DeepSurv and PMF-NN models provide clinically informative predictions. Our developed models outperformed the Cox proportional hazards (CPH) with supervised principal component analysis (SPCA) from a separate study in terms of IBS.
[18F]-PSMA-1007-PET for evaluation of kidney function
Authors: Rassek Philipp , Schäfers Michael , Rahbar Kambiz , Backhaus Philipp 
Published: October 2022

Abstract
Purpose
Prostate-specific membrane antigen (PSMA) is present in the proximal tubule cells of the kidneys. This results in high renal tracer uptake in PSMA-PET, which may contain useful information on renal function. As part of the clinical evaluation for [177Lu]-PSMA therapies, patients undergo PSMA-PET and often additional [99mTc]-mercapto-acetyltriglycine (MAG3) scintigraphy to assess renal function. Aim of this study was to evaluate estimation of renal function with [18F]-PSMA-1007-PET/CT (PSMA-PET) by comparison to synchronous MAG3-scintigraphies.
Methods
We retrospectively investigated 73 prostate cancer patients with 93 synchronously available PSMA-PET/CT, MAG3-scintigraphies and serum creatinine. For determination of split renal function in PSMA-PET/CT, we evaluated the relative unilateral total renal PSMA uptake, i.e. SUVmean multiplied by the renal volume (SRFPSMA-TOTAL) and relative unilateral maximal standardized uptake value (SRFSUV). These were compared to MAG3 split renal function (SRFMAG3) using Pearson correlation and receiver operating characteristics analysis. For determination of global renal function, correlation of bilateral total renal PSMA uptake with MAG3 tubular excretion rate and serum creatinine was assessed.
Results
SRFMAG3 was strongly correlated with SRFPSMA-TOTAL (r= 0.872, p<0.001) and with SRFSUV (r=0.815, p<0.001). Relevant abnormalities of SRFMAG3 (unilateral renal function < 25 %) could be detected with sensitivities and specificities of 90% and 92% for SRFPSMA-TOTAL, and 80% and 95% for SRFSUV. Measures of absolute renal function were only weakly correlated with bilateral total renal PSMA uptake.
Conclusion
Renal [18F]-PSMA-1007 uptake allowed to quantify renal split function with good accuracy based on SRFPSMA-TOTAL or SRFSUV.