bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitral paravalvular leak 3D printing from 3D-transesophageal echocardiography – proof of concept and preparation flowchart
Authors: Jędrzejek Marek , Kozłowski Michał , Jadczyk Tomasz , Peszek-Przybyła Ewa , Pysz Piotr , Wojakowski Wojciech , Smolka Grzegorz 
Published: June 2022

Abstract
Paravalvular leaks (PVLs) can be detected in almost 15% of patients after mitral valve prosthesis implantation. This complication can result in congestive heart failure and hemolysis. Despite advancements in non-invasive imaging, percutaneous closure of PVLs is not always successful. Therefore, efforts are made to improve treatment outcomes by using 3D-printed models of defects as pre-procedural support for interventional cardiologists. Retrospectively, 3D-TEE recordings of 8 patients with clinically significant mitral PVLs were analysed. Qlab Software was used to export DICOM images of each PVL channel, including surrounding tissue. Image segmentation was performed in 3D Slicer, a free, open-source software package used for imaging research. Models were printed to actual size with the polyjet Stratasys Objet 30 printer with a transparent, rigid material. Duration of model preparation and printing, as well as total cost were calculated. Mean total time of model preparation was 430,5 ± 196 min. 3D-printing from 3D-TEE is technically feasible. Both shape and location of PVLs are preserved during model preparation and printing. It remains to be tested if 3D-printing would improve outcomes of percutaneous PVL closure.
Regulation of human trophoblast gene expression by endogenous retroviruses
Authors: Branco Miguel , Frost Jennifer , Amante Samuele , Okae Hiroaki , Jones Eleri , Ashley Brogan , Lewis Rohan , Cleal Jane , Arima Takahiro , Maffucci Tania , Caley Matthew 
Published: June 2022

Abstract
The placenta is a fast-evolving organ with large morphological and histological differences across eutherians, but the genetic changes driving placental evolution have not been fully elucidated. Transposable elements, through their capacity to quickly generate genetic variation and affect host gene regulation, may have helped to define species-specific trophoblast gene expression programmes. Here, we assessed the contribution of transposable elements to human trophoblast gene expression as enhancers or promoters. Using epigenomic data from primary human trophoblast and trophoblast stem cell lines, we identified multiple endogenous retrovirus families with regulatory potential that lie close to genes with preferential expression in trophoblast. These largely primate-specific elements are associated with inter-species gene expression differences, and are bound by transcription factors with key roles in placental development. Using genetic editing we demonstrated that several elements act as transcriptional enhancers of important placental genes, such as CSF1R and PSG5. We also identified an LTR10A element that regulates ENG expression, affecting secretion of soluble ENG, with potential implications for preeclampsia. Our data show that transposons have made important contributions to human trophoblast gene regulation, and suggest that their activity may affect pregnancy outcomes.
Global research trends on diseases in the elderly population: a visualization analysis
Authors: Zhao Lei , Zhao Min 
Published: June 2022

Abstract
Background
Currently, populations around the world are rapidly ageing. Hence, the number of scientific publications on diseases in the elderly population (aged 60 and over) has been expanding.
Objective
This study aimed to use bibliometric analysis method to illustrate the global research trends over the past 20 years and to summarize the research hotspots that are likely to be addressed in future studies.
Methods
A literature search for medical publications on diseases in the elderly population in the period from 2000 to 2021 was conducted in the Web of Science Core Collection on April 20, 2022. Visualization analysis was performed using VOSviewer software (version 1.6.16).
Results
A total of 12,108 publications of the period from 2000 to 2021 were retrieved. The annual publication output grew gradually from 177 in 2000 to 1,236 in 2021. Articles (n = 10,264, 84.77%) were the most common type of documents. The USA issued the largest number of publications (n = 3,632, 30.00%) and had the most citations (citations = 141,230), with the United States Department of Health and Human Services making the largest contribution (n = 1,904, 15.73%). The Lancet had the most citations (citations = 28,548). A total of 545 meaningful topics were identified and further classified into 5 different clusters. The keywords ‘COVID-19’, ‘muscle mass’, ‘depression’ and ‘non-alcoholic fatty liver disease’ have drawn substantial attention in recent years, indicating that these areas will be the centre of attention in future studies on the elderly population.
Conclusions
This study provides an unbiased visualization analysis of this topic in a scale-efficient manner and includes directions for further research.
Non-covalent immobilized sultone based protic ionic liquids on nano silica sulfuric acid as novel heterogeneous catalysts for the efficient synthesis of tetrazolopyrimidines
Authors: Taib Layla A. , Keshavarz Mosadegh 
Published: June 2022

Abstract
In this research work, a novel strategy for the heterogenization of sultone based acidic ionic liquids via their non-covalent immobilization on nano silica sulfuric acid is reported and the catalytic ability of the prepared heterogeneous catalysts for the synthesis of tetrazolopyrimidines derivatives is investigated. The physicochemical properties of the prepared catalysts were studied using thermal and spectroscopic techniques such as 1H and 13C NMR, FTIR, SEM, TEM, TGA, EDX and BET. In the following, tetrazolopyrimidine derivatives were synthesized in the presence of the prepared heterogeneous supported acidic ionic liquids on nano silica sulfuric acid in a three-component reaction of aromatic aldehydes, 5-aminotetrazole and active methylene compounds. The obtained results showed that the supported protic ionic liquids have better catalytic activity than their non-supported forms. Moreover, the recoverability, reusability and thermal stability of the acidic ionic liquids were also significantly improved by immobilization on nano silica sulfuric acid.
The Association Between Dietary Calcium Intake and Hyperuricemia Among Chinese Adults
Authors: Zhang Huanxiang , Gu Xia , Wang Shanjie , Sui Xiaofang , Qiu Hongbin , Zhang Yiying 
Published: June 2022

Abstract
Background
Previous studies demonstrated that dietary nutrients intake is associated with the risk of hyperuricemia. Calcium, acting as an essential nutrient in human body, therefore, may be associated with hyperuricemia. However, until now, no studies have independently assessed the association between dietary calcium intake.
Methods
7,606 participants aged 18–94 years from the China Health and Nutrition Survey (CHNS) in 2009 were extracted. Three consecutive 24 dietary recalls were used to assess all dietary intake. Multivariable logistic regression and restricted cubic spline (RCS) was performed to evaluate the relationship between dietary calcium intake and hyperuricemia after adjustment for confounders.
Results
The crude prevalence of hyperuricemia was 15.67% (20.51% for male, 11.66% for female). A positive association between dietary calcium intake and risk of hyperuricemia even in the fully adjusted model. Compared with the lowest quintile, the odds rations for hyperuricemia were 1.130 [95% confidence interval (CI): 0.885–1.441], 1.257 (95% CI: 0.987–1.600), 1.309 (95% CI: 1.020–1.680), and 1.552 (95% CI: 1.180–2.041) for the second to highest quintiles of dietary calcium intake, respectively (P for trend = 0.015), and a positive dose-response relationship was observed.
Conclusions
Higher dietary calcium intake is associated with an increased risk of hyperuricemia independent of some major confounding factors.
TAT-W61 peptide reduces the injury by inhibiting the binding S100b to the V-domain of Rage during ischemic stroke
Authors: Liang Jia , Li Hui , Ma Xiang-Yu , Liu Dan , Chen Yu-Ling , Feng Qian , Zhang Zhen , Wen Xiang-Ru , Wang Nan , Song Yuan-Jian 
Published: June 2022

Abstract
Ischemic stroke is a nervous system disease associated with high mortality and morbidity rates. Currently, there are no neuroprotective drugs for this disease. S100 calcium-binding protein b (S100b), which is abnormally expressed in the cerebrospinal fluid of patients, can bind to various other proteins and exacerbate the progression of stroke; receptor for advanced glycation end-products (Rage) is one of these proteins. To inhibit the occurrence of this interaction, a peptide (TAT-W61) based on the V-domain of S100b that interacts with Rage was produced. This study aimed to use TAT-W61 to reduce the levels of inflammation and apoptosis after ischemic stroke and to improve learning memory and motor dysfunction. In this study, S100b and Rage expression increased after ischemic stroke. Possible binding sites of S100b on Rage were mapped through molecular docking and polar–contact assays. In immunoprecipitation experiments, TAT-W61 inhibited the binding of S100b to Rage after ischemic stroke. TAT-W61 peptide, derived from Rage, could reduce the inflammatory and apoptotic response caused by ischemic stroke. It may also improve the infarct volume of the pathological injury and reduce the apoptotic rate. In particular, the TAT-W61 peptide could significantly improve learning, memory, and motor dysfunction in an ischemic stroke model. TAT-W61 peptide may help alleviate ischemic stroke injury.
Multi-resource potentiality and multi-hazard susceptibility assessments of the central west coast of India applying machine learning and geospatial techniques
Authors: PRASAD PANKAJ , Loveson Victor Joseph , Mandal Sourav , Chandra Priyankar , Kulimushi Luc Cimusa 
Published: June 2022

Abstract
The understating of natural resources and hazards are very fundamental from spatial perspective to reduce resource losses and human lives. For this, the main aim of the current research is to synthesize the three natural resources (groundwater, wetland, and forest) into one multi-resource potentiality map and three natural hazards (flood, landslide, and shoreline erosion) into one multi-hazard susceptibility map. In order to achieve this goal, several inventories, geo-environmental factors, satellite bands and indices have been taken as input datasets. Random forest (RF) model has been considered for individual mapping (except shoreline erosion mapping) due to its high precision. For shoreline erosion mapping, digital shoreline analysis system (DSAS) model has been employed. The accuracies of the model have been determined from the area under receiver operating characteristic (AUROC) curve, producer accuracy (PA), and user accuracy (UA). RF model has more than 90% of prediction accuracy in different resource and hazard mapping whereas for the forest mapping PA and UA are 90% and 80%, respectively. The results of this study for multi-resource mapping show forest (24.05%) and groundwater (22.28%) as the major resources whereas flood (17.27%) is the most destructive in comparison to other hazards. The multi-resource and multi-hazard maps of the research area provide an important tool to land managers and policymakers for sustainable development and management.
Integrated Bioinformatics Analysis for Potential Target Genes of In-Stent Restenosis
Authors: Liu Chenxi , Kuang Yuanyuan , Liu Yubo , Peng Yi , Zhang Yinzhuang , Gao Haodong , Yang Xiangyu , Tang Jia , Ma Li , Ma Qilin 
Published: June 2022

Abstract
Background: In-stent restenosis (ISR) is a common complication after percutaneous coronary intervention (PCI), which greatly affects the patients after PCI. Here, we extracted the left internal mammary artery (LIMA) from 4 coronary artery bypass graft (CABG) patients with stable angina and 3-vessel coronary atherosclerotic lesions, implanted bare-metal stents (BMS) or paclitaxel drug-eluting stents (PES), and analyzed their tissue differentially expressed genes (DEGs) to explore the possible mechanism and pathway of ISR formation.Methods: To figure out the effects of BMS and PES on vascular tissue and find the potential target genes for ISR, we explored DEGs from GSE19136 by comparing LIMA tissues implanted two stents. All DEGs were divided into up-regulating group and down-regulating group and enriched respectively in Gene Ontology (GO) terms and Kyoto Encyclopedia of Genesand Genomes (KEGG). Gene set enrichment analysis (GSEA) was aimed at all gene expression data. Constructing the PPI network of two groups of DEGs, we identified several hub genes and predicted their regulating miRNAs and key transcription factors (TFs) regulators in the online database. Additionally, we used RT-PCR to validate hub genes expression in two models of endothelial (EC) inflammation and vascular smooth muscle (VSMC) proliferation, which simulated the mechanism of ISR.Results: The datasets had a total of 759 DEGs (log2|foldchange(FC)| > 1 and P value < 0.05) identified, including 389 up-regulated and 370 down-regulated DEGs, Bioinformatics analysis result shows relevant genes function and identified hub genes and their regulating factors. Finally, RT-PCR validated that there were significant differences in the expression of LIF, CENPE, KIF11, MMP3, MCM10, and ARHGAP11A in the constructed cell model of EC inflammation, while GTSE1, GINS2, RRM2 and CKAP2L in the model of VSMC proliferation (P value < 0.05).Conclusion: Here, using comprehensive bioinformatics analysis and functional enrichment, We found the potential target genes (LIF, CENPE, KIF11, MMP3, MCM10, ARHGAP11A, GTSE1, GINS2, RRM2 and CKAP2L) inherent ISR by comparing LIMA tissues implanted with different stents in GSE19136. We identified several potential genes, which are worthy of new exploration for the prevention of ISR in the future.
Quantify retinal structure in high-altitude residents with and without High altitude polycythemia
Authors: Ma Jinlan , Niu Haoyu , Han Changjing , Qu Yi 
Published: June 2022

Abstract
Background
To assess retinal structural parameters in high-altitude (HA) residents with and without high altitude polycythemia (HAPC) and to elucidate the relationship between retinal structural parameters and hemoglobin (HGB).
Methods
This cross-sectional study included 55 HAPC patients and 52 healthy HA residents. Retinal structural parameters including retinal nerve fiber layer (RNFL) thickness, optic nerve head (ONH) parameters and retinal vessels diameter were acquired from spectral domain optical coherence tomography (SD-OCT) and ImageJ software, and compared between HAPC group versus healthy HA group. The associations between retinal parameters and HGB were assessed by Pearson correlation analyses.
Results
In comparison of HAPC group versus healthy HA group, RNFL thickness was significant thicker in the nasal quadrant of the optic disc in HAPC group (74.82 ± 14.4 VS. 66.06 ± 13.71µm, P = 0.002). Bigger rim area, bigger disc area and bigger cup volume were also observed in HAPC group (all P < 0.05). Moreover, retinal venous diameters (RVD) of all four quadrants dilated significantly (all P < 0.05) and retinal arterial diameters (RAD) dilated only in superior nasal quadrant in HAPC group (0.08 ± 0.01 VS. 0.07 ± 0.01mm, P = 0.002). No associations between HGB and retinal parameters were found (all P > 0.05).
Conclusions
long-term HA exposure secondary HAPC could result in slightly thickened RNFL, enlarged ONH and dilated retinal veins. However, increased blood viscosity caused by HAPC should not be responsible for those changes. This study deepens the understanding of the impact of HA environment on retina.
The Augmented Reality affective training increases the care communication skill and empathy
Authors: Nakazawa Atsushi , Iwamoto Miyuki , Kurazume Ryo , Nunoi Masato , Kobayashi Masaki , Honda Miwako 
Published: June 2022

Abstract
It is important for caregivers of people with dementia (PwD) to have good patient communication skills as it has been known to reduce the behavioral and psychological symptoms of dementia (BPSD) of PwD as well as caregiver burnout. However, acquiring such skills often requires one-on-one affective training which can be costly. In this study, we propose affective training using augmented reality (AR) for supporting the acquisition of such skills. The system uses a see-through AR glasses and a nursing training doll to train the user in both practical nursing skills and affective skills such as eye contact and patient communication. The experiment was conducted with 39 nursing students. The participants were assigned to either the Doll group, which only used a doll for training, or the AR group, which used both a doll and the AR system. The results showed that eye contact significantly increased and the face-to-face distance and angle decreased in the AR group, while the Doll group had no significant difference. In addition, the empathy score of the AR group significantly increased after the training. Upon analyzing the correlation between personality and changes of physical skills, we found a significant positive correlation between the improvement rate of eye contact and extraversion in the AR group. These results demonstrated that affective training using AR is effective for improving caregivers’ physical skills and their empathy for their patients. We believe that this system will be beneficial not only for dementia caregivers but for anyone looking to improve their general communication skills.
Role of Maraviroc and/or Rapamycin in Aging Liver Processes
Authors: Martinez Laura Perez , Romero Lourdes , Verdugo-Sivianes Eva M , Muñoz-Galvan Sandra , Rubio-Mediavilla Susana , Amiama Ana , Carnero Amancio , Blanco Jose Ramon 
Published: June 2022

Abstract
Background: Cellular senescence and low-grade inflammation favor the acceleration of aging. The liver is an essential metabolic organ because changes related to its function are related to age-related diseases. The objective of this study was to evaluate the effects of maraviroc (MVC) and/or rapamycin (RAPA) on liver tissue in an experimental model of aging in mice.Materials and methods: Eighty male homozygous IL10KO mice were randomly assigned to one of 4 groups (n= 20): i) IL10KO group (IL10KO); ii) IL10KO mice that received MVC in drinking water (MVC group), iii) IL10KO mice that received RAPA in drinking water (RAPA group), and iv) MVC-RAPA group that received MVC and RAPA in drinking water. Liver samples were analyzed. Gene expression quantification, western blotting and histopathological analyses were also performed.Results: The proinflammatory cytokines IL-6 and IL-18 were decreased in the MVC and MVC/RAPA groups, and TNF-α was decreased in all therapeutic groups. Galactosidase beta-1, a senescence biomarker, was also significantly reduced in all therapeutic groups, as were NK-kB1 and NF-kB2. In all groups, mTOR and CCL5 were significantly reduced. CCR5 expression was decreased in the MVC and MVC/RAPA groups.Conclusions: MVC and RAPA may protect against some factors involved in liver aging. More studies will be necessary to verify their clinical applications.
Groundwater Purification Using Gamma Irradiation under Oxidation and Reduction Conditions
Authors: El-Toony Mohamed M. , Rajab Ahmed H. , Eid Ghada A. , Maziad Nabial M. 
Published: June 2022

Abstract
Gamma irradiation was applied for the elimination of different pollutants in groundwater. Two techniques of irradiation have been studied. The first technique, irradiation-reduction, resulted in un-soluble metals forming aggregations, such as cadmium, mercury, and lead, which can be separated out of the solution. Different parameters, such as different hydroxyl scavengers and different gasses, were used for water de-aeration. Metal ion removal yields were 99.4%, 97.8%, and 96.9%, for Cd2+, Hg2+, and Pb2+ respectively at the best-studied conditions. The second technique was irradiation coupled with ozonization which performed organics degradation. The procedure can reduce the values of turbidity, chemical oxygen demand (COD), biological oxygen demand (BOD), total organic carbon (TOC), and total dissolved solids (TDS). The bacterial colony counts, and rotavirus, have diminished. A constant value of COD/BOD ratios at different purification steps was about 1.2. For the sake of conferment, this methodology of degradation of a simulated organic detergent and antibacterial such as triclosan has been studied. Degradation of triclosan, using reduction techniques, produced fragments dangerous to human health which were ensured by high values of COD and TOC. These fragments were completely degraded using the two techniques (reduction followed by oxidation) which were conducted to attain the least COD and TOC values. The two techniques of irradiation proved to be successful method for people highly sensitive to pollutants in water consumed.
Socio-behavioral correlates of pre-exposure prophylaxis use and correct adherence in men who have sex with men in West Africa
Authors: Eubanks August , Coulibaly Bakary , Keita Bintou Dembélé , Anoma Camille , Dah Ter Tiero Elias , Mensah Ephrem , Kaba Sékou , Lokrou Kpassou Julien , Ouedraogo Faïçal Rodrigue , Badjassim Alèda M. Fidèle , Maradan Gwenaëlle , Bourrelly Michel , Mora Marion , Riegel Lucas , Castro Daniela Rojas , Yaya Issifou , Spire Bruno , Laurent Christian , Group Luis Sagaon-Teyssier on behalf of the CohMSM-PrEP Study 
Published: June 2022

Abstract
BackgroundMultiple barriers compromise pre-exposure prophylaxis (PrEP) engagement (i.e., use and adherence) in men who have sex with men (MSM). In low/middle-income countries, little is known about PrEP engagement in this population. In West Africa, the CohMSM-PrEP study was one of the rare interventions providing PrEP to MSM. We estimated PrEP use and correct adherence rates in CohMSM-PrEP, together with associated factors.MethodsCohMSM-PrEP recruited MSM in four community-based clinics in Mali, Côte d’Ivoire, Burkina Faso, and Togo. Quarterly follow-up included collecting socio-behavioral data, and providing a comprehensive HIV prevention package, PrEP (daily or event-driven), and peer educator (PE)-led counselling (prevention and adherence). Multivariate generalized estimating equations (GEE) models were used to identify factors associated with self-reported i) PrEP use and ii) correct PrEP adherence during participants’ most recent anal intercourse.Results520 participants were retained for the present analysis. Median follow-up time was 12 months (IQR 6-21). Of the 2839 most recent anal intercourses declared over the follow-up period, PrEP use was self-reported for 1996 (70%), and correct PrEP adherence for 1461 (73%) of the latter. PrEP use was higher in participants who attended participating clinics outside of scheduled visits (adjusted odds ratio (aOR) [95% Confidence Interval, CI], p-value; 1.32 [1.01-1.71], 0.040), and in those who practiced condomless anal sex (1.86 [1.54-2.24], <0.001). Correct adherence was higher in those who often contacted PE outside of scheduled visits (2.16 [1.01-4.64], 0.047) and in participants who adopted receptive/versatile sexual positions with stable partners (1.36 [1.03-1.81], 0.030). Instead, it was lower in event-driven users with a difficult/very difficult financial situation (comfortable/just making ends meet & daily, 4.19 [2.56-6.86], <0.001; difficult/very difficult & daily, 6.47 [4.05-10.30], <0.001; comfortable/just making ends meet & event-driven, 1.63 [1.22-2.17], 0.001), and in participants who felt alone (0.76 [0.58-0.99], 0.042).ConclusionsCommunity-based clinic attendance and PE contact outside of scheduled visits were both associated with higher PrEP engagement. Some socially and economically marginalized participants struggled with adherence. Providing adherence support is crucial for PrEP effectiveness, especially in hostile sociocultural contexts. As PrEP scale-up continues in West Africa, we recommend using comprehensive community-based interventions to ensure adequate PrEP engagement.