bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a MET-targeted single-chain antibody fragment as an anti-oncogene targeted therapy for breast cancer
Authors: Vafaei Rana , Khaki Zohreh , Salehi Malihe , Jalili Neda , Esmailinejad Mohammad Reza , Muhammadnajad Ahad , Nassiri Seyed Mahdi , Vajhi Alireza , Kalbolandi Shima Moradi , Mirzaei Roya , Farahmand Leila 
Published: November 2022

AbstractThe usage of monoclonal antibodies (mAbs), as a matter associated with the biopharmaceutical industry, is increasingly growing. Harmonious with this concept, we designed the exquisitely modeled anti-MET scFv against breast cancer by gene cloning, and expression using a bacterial host. Herein, we developed a recombinant scFv against MET and examined its preclinical efficacy for the reduction of tumor growth, invasiveness and angiogenesis in vitro and in vivo. Expressed anti-MET scFv demonstrated high binding capacity (48.8%) toward MET-overexpressing cancer cells. The IC50 value of anti-MET scFv against MET-positive human breast cancer cell line (MDA-MB-435) was 11.4 nM whereas this value was measured as 47.01 nM in MET-negative cell line BT-483. Similar concentrations could also effectively induce apoptosis in MDA-MB-435 cancer cells. Moreover, this antibody fragment could reduce migration and invasion in MDA-MB-435 cells. Grafted breast tumors in Balb/c mice showed significant tumor growth suppression as well as reduction of blood-supply in response to recombinant anti-MET treatment. Histopathology and immunohistochemical assessments revealed higher rate of response to therapy. In our study, we designed and synthetized a novel anti-MET scFv which could effectively suppress MET-overexpressing breast cancer tumors.
Implementing an Immunotherapy Toxicity (IOTOX) GI Service Improves Outcomes in Patients with Immune Mediated Diarrhea and Colitis
Authors: Saji Alice , Chopra Maneera , Jacob Jake , Altan Mehmet , Alhalabi Omar , Shah Amishi Yogesh , Qiao Wei , Wang Yinghong , Thomas Anusha 
Published: November 2022

Abstract
Purpose
Immune checkpoint inhibitors (ICIs) have revolutionized cancer therapy but can lead to GI toxicity, termed immune mediated diarrhea and colitis (IMDC). Standardization of IMDC management and early GI consultation is imperative to control symptoms and prevent delays in cancer care. Therefore, we implemented an inpatient algorithm and a focused IOTOX GI service to measure outcomes.
Methods
Patients who received ICIs and were hospitalized with severe IMDC were grouped into a pre-interventional cohort in 2017, followed by implementation of the standardized algorithm in 2018, and then a post-interventional cohort of patients in 2019. Clinical data and patient outcomes were compared using univariate and multivariate analysis to determine the morbidity,, and overall survival.
Results
Our sample comprised 126 hospitalized patients with IMDC, with 59 patients in the pre-interventional 2017 cohort, and 67 patients in the post-interventional 2019 cohort. We found no significant differences in the clinical severity of IMDC symptoms between the two cohorts (p = 1.03) or median time from ICI exposure to development of IMDC (p = 0.495 respectively). After implementing the standardized algorithm, we observed higher rates of GI consultation (p < 0.001) in the post-treatment group. Patients in the post-treatment cohort showed decreased time to clinical remission (4 vs 10 days, p = 0.046), higher rate of GI follow up after hospital discharge (p = 0.038), fewer hospital re-admissions (p = 0.002), and significantly fewer recurrences of IMDC symptoms (p = 0.002). Overall survival was significantly higher for at least 2 years in patients who followed with GI post-discharge compared to those without follow up (p = 0.003).
Conclusion
Prompt GI consultation and monitoring of IMDC using a regimented approach can provide efficacious management, decrease time to clinical remission of symptoms, decrease re-admissions to the hospital, and improve overall patient outcomes.
Reforestation of grey mangroves (Avicennia marina) along the northern coasts of the Persian Gulf
Authors: Farshid Zahra , Balef Reshad Moradi , Zendehboudi Tuba , Dehghan Niloofar , Mohajer Fatemeh , Kalbi Siavash , Hashemi Alireza , Afshar Alireza , Bafghi Tabandeh Heidari , Banshi Haniyeh , Tamadon Amin 
Published: November 2022

Abstract
Every ecosystem supports the lives of living things by providing direct and indirect benefits and services. Among these areas, mangrove forests are one of the most fertile ecosystems in the intertidal zones. These natural forests are one of the coastal ecosystems that not only provide a source of food for humans and animals, but also play a major role in protecting and stabilizing coastlines and controlling climate. Mangroves are a breeding ground and a safe habitat for a variety of marine vertebrates and invertebrates. Increasing gray mangrove forests, Avicenna marina along the north coasts of the Persian Gulf was performed. This article explains the experienced methods of propagation and foresting of this species. For the first time in the Persian Gulf, we reported a five-year evaluation of reforestation method of grey mangrove. Reforestation in the highest latitude of natural forests in the Persian Gulf did not have any side effects on growing of gray mangrove in the selected sites. As a result, increasing the area of ​​gray mangrove forests in the Persian Gulf improves the ecosystems of the tidal area. Increasing the chances of fishing and improving the economic conditions of the local communities around this bay will be some of the results of mangrove forestation. Furthermore, this is the first report of a simple method to train all the steps of grey mangrove reforestation.
Creation Techniques and Styles of Chinese Piano Works Supported by Edge Cloud Computing Technology
Authors: Liu Jingyuan 
Published: November 2022

Abstract

The purpose is to explore the application of the deep learning (DL) algorithm and edge cloud computing technology in the creation and innovation of Chinese piano music, understand the development history of Chinese piano music, the evolution of creative techniques, style characteristics, and innovation. Firstly, on the basis of the combination of traditional and modern techniques, using scientific research methods such as surveys and documents, a preliminary discussion and arrangement of a large number of piano music works are carried out from the three aspects of creation characteristics, techniques, and styles. Secondly, according to the characteristics of edge cloud computing technology, the application and embodiment of this technology in Chinese piano music creation and innovation are explained. Moreover, the DL algorithm is further introduced and improved to implement the piano music creation technique and style recognition model based on the Convolution, Long-short Term Memory, and Deep Neural Network (CLDNN) algorithm. Finally, the performance of the constructed model is analyzed through comparative experiments. The results reveal that by sorting relevant literature, the five major periods of Chinese piano music experience and a large number of classic piano repertoires can be clearly sorted out. It can be understood that piano music creation techniques and style changes will be an endless process, requiring more arduous learning by composers and theorists. Further analysis of the constructed model denotes that the recognition accuracy of the proposed model algorithm reaches 91.33%, which is obviously better than other algorithms. Therefore, the integration of the DL algorithm and other methods has certain research significance for exploring the creative techniques and style innovation of Chinese piano works.
Sensitivity of renal cell carcinoma to cuproptosis and cuproptosis related genes FDX1 combined with DLAT as an immunological and prognostic biomarker
Authors: Li Hongfang , Zhu Neng , Shi Yaning , Liu Qiaofa , Gu Jia , Qin Li 
Published: November 2022

Abstract
Background
Targeting cuproptosis is being considered as a novel and promising therapeutic strategy for the prevention of tumors. Nonetheless, the potential role of cuproptosis and its related genes in renal cell carcinoma (RCC) remains elusive.
Methods
Cuproptosis differential genes (CDGs) in clear cell renal cell carcinoma (ccRCC) were extracted by GSE53757 dataset and cuproptosis-related genes. The role of CDGs was comprehensively analyzed by multiple public databases. CDGs expression in clinical samples was verified by western blot assay. Cuproptosis inducer elesclomol was utilized to explore the sensitivity of ccRCC to cuproptosis. Drug targets of CDGs were predicted and validated through DrugBank database and molecular docking, respectively.
Results
The results found that FDX and DLAT exhibited significantly low expression in ccRCC tissues, which was validated in clinical samples. FDX1 and DLAT acted as independent prognostic factors for poor survival in ccRCC patients and showed a strong positive correlation. Functional analysis of differentially expressed genes that were positively or negatively correlated with both FDX1 and DLAT in ccRCC demonstrated that acetyl-CoA biosynthetic process and acetyl-CoA metabolic process were remarkably affected. The methylation levels and sites of FDX1 and DLAT genes were dramatically correlated with overall survival (OS) in ccRCC patients. FDX1 and DLAT expression was strongly correlated with immune infiltration and immune checkpoints. Importantly, elesclomol significantly induced cell death in 786-O and A498 cells, and the expression of FDX1 and DLAT proteins was also markedly up-regulated. Docking results indicate that mitotane, adicicol and dihydrolipoic acid fit reasonably well in the FDX1 and DLAT ligand binding pockets, which may be potential drug targets for FDX1 and DLAT genes.
Conclusion
The present study implies for the first time the sensitivity of ccRCC to cuproptosis and the combination of targeted FDX1 and DLAT may be a novel therapeutic strategy to induce cuproptosis in ccRCC.
Different selection criteria may relax competition for denning sites between expanding and endemic predators on the low-Arctic tundra
Authors: Moizan Audrey , Rodrigues Chloe Warret , Roth James D. 
Published: November 2022

Abstract

Climate warming is favoring the expansion of non-native species onto the Arctic tundra, where they may compete over resources with native species. In the harsh tundra conditions, sympatric red foxes (Vulpes vulpes) and Arctic foxes (Vulpes lagopus) may compete over denning sites, which are important for their reproduction and survival. We studied den selection by red and Arctic foxes in spring and summer, and their possible competition over this resource in an ecotone near Churchill, Manitoba, on the west coast of Hudson Bay, by examining patterns of den occupancy related to den characteristics and spacing patterns between neighbors. Based on 11 years of occupancy data for 42 tundra dens, we determined that red and Arctic foxes favored dens based on shelter quality in both spring and summer, rather than proximity of specific habitats (and thus specific prey). Mechanisms of den selection differed between species, which may promote co-existence, and areas of high den density were avoided by red foxes and preferred by Arctic foxes. We did not find evidence of exclusion of Arctic foxes by red foxes: spacing patterns showed that foxes spaced themselves based on their need for space, territoriality and food availability but not interference. In the current abiotic Arctic conditions, taiga species settling on the tundra could coexist with tundra endemics, at given density thresholds of both competitors. As Arctic conditions may become milder, an increase in newcomer abundance could disrupt the current balance that favors species coexistence.
Standard deviation of CT radiomic features among malignancies in each individual: Prognostic ability in lung cancer patients
Authors: Hongwei Si , Xinzhong Hao , Huiqin Xu , Shuqin Xue , Ruonan Wang , Li Li , Jianzhong Cao , Sijin Li 
Published: November 2022

Abstract
Purpose
It was reported that individual heterogeneity among malignancies (IHAM) might correlate well to the prognosis of lung cancer; however, seldom radiomic study is on this field. Standard deviation (SD) in statistics could scale average amount of variability of a variable; therefore, we used SD of CT feature (FeatureSD) among primary tumor and malignant lymph nodes (LNs) in an individual to represent IHAM, and its prognostic ability was explored.
Methods
The enrolled patients who had accepted PET/CT scans were selected from our previous study (ClinicalTrials.gov, NCT03648151). The patients had primary tumor and at least one LN, and standardized uptake value of LN higher than 2.0 and 2.5 were enrolled as the cohort 1 (n = 94) and 2 (n = 88), respectively. FeatureSD from the combined or thin-section CT were calculated among primary tumor and malignant LNs in each patient, and were separately selected by the survival XGBoost method. Finally, their prognostic ability was compared to the significant patient characteristics identified by the Cox regression.
Results
In the univariate and multivariate Cox analysis, surgery, target therapy, and TNM stage were significantly against OS in the both cohorts. In the survival XGBoost analysis of the thin-section CT dataset, none FeatureSD could be repeatablely ranked on the top list of the both cohorts. For the combined CT dataset, only one FeatureSD ranked in the top three of both cohorts, but the 3 significant factors in the Cox regression were not even on the list. Both in the cohort 1 and 2, C-index of the model composed of the 3 factors could be improved by integrating the continuous FeatureSD; furthermore, that of each factor was obviously lower than FeatureSD.
Conclusion
Standard deviation of CT features among malignant foci within an individual was a powerful prognostic factor in vivo for lung cancer patients.
A modified laparoscopic surgery for congenital duodenal membranes in neonates
Authors: Hao Chen , Xiaogang Sun , yingjun Wang , Huiming Zheng , Wang Ruoyi , Chen Weixiu 
Published: November 2022

Abstract
Purpose: To investigate the effectiveness and safety of a modified laparoscopic surgery for neonatal duodenal membrane.
Methods: A total of 23 neonates with congenital duodenal membrane were selected from Feb 2012 to Feb 2022. The patients were divided into the control group receiving duodenal membranes resection or rhomboid anastomosis, and the observation group receiving laparoscopic membranes incision, longitudinal incision and transverse suture for the duodenum. The clinical data of all cases were retrospectively reviewed. The operative time, estimated blood loss, time to initial oral feeding, length of stay (LOS), anastomotic obstruction, and incision dehiscence were compared between the two groups.
Results: The control group contained 12 children, with average operative time of 133.6±12.8 min, an estimated blood loss of 9.8±2.7 ml, a period of 7.7±2.2 days to initial oral feeding, and an LOS of 12.9±3.8 days. Bilious vomiting was observed in one patient at 15 days after surgery and cured with conservative treatment, and incision dehiscence in two patients and cured by tension-reduced suture. The observation group contained 11 children, with an operative time of 137.7±12.7 min, an estimated blood loss of 7.5±1.9 ml, a period of 6.1±0.9 days to initial oral feeding was, and an LOS of 11.2±2.0 days. There were no conversion to laparotomy, intraoperative or incision complications. Bilious vomiting was observed in one patient at 9 days after surgery and cured with conservative treatment. The period to initial oral feeding and LOS in the observation group were lower than those in the control group (P <0.05). The incidence of incision infection in the control group was 16.7%. The patients were followed up for 3~36 months (mean 15.9 months), all showing normal development.
Conclusion: The modified laparoscopy is effective and safe to treat neonates with congenital duodenal membranes.
Study of Dilated Cardiomyopathy and Its Correlation to Age, Sex, Risk Factors, and Clinical Manifestations in Patients of Damascus Hospital.
Authors: Gherli Haia Marwan , Ahmad Aya , Shamieh Marwan , Alkharrat Leen 
Published: November 2022

Abstract
Background and Aim:
The purpose of this study was to the identification of clinical manifestations and risk factors for patients with dilated cardiomyopathy in order to reduce complications, save patients' lives, and support the health system in Syria.
Methods:
This retrospective observational cohort study was conducted at the department of cardiology at Damascus Hospital in Damascus, Syria. We identified 125 patients with a diagnosis of dilated cardiomyopathy between 2013 and 2022. Patients’ clinical data were documented using Google Forms, and it was analyzed using SPSS version 25.
Results:
The most common age group in our study was the ages between 51-69 years which constituted 42%. As for sex, males constituted 69% of the sample, while females were only 31%. About 55% of the sample were smokers, and only 14% of patients were alcohol consumers. Patients who were diagnosed with DMC accounted for 80% of the sample. Symptoms included dyspnea which was the most common symptom among patients, with a percentage of 85%. The only statistical correlation with dilated cardiomyopathy was diabetes, as the percentage of patients with dilated cardiomyopathy who were suffering from diabetes was 72%, compared to only 28% of those who suffer from diabetes and those who do not have dilated cardiomyopathy.
Conclusion:
DCM is a non-ischemic cardiomyopathy with structural and functional cardiomyopathy abnormalities. It is necessary not to neglect the family history of cardiac diseases due to its importance in diagnosing heart diseases especially in young patients.
Acteoside (Verbascoside): A Prospective Therapeutic Alternative against
Hepatocellular Carcinoma by Inhibiting the Expression of AXL, FGFR,
BRAF, TIE2 and RAF1 Targets
Authors: Kityania Sibashish , Nath Rajat , Nath Deepa , Patra Jayanta Kumar , Talukdar Anupam Das 
Published: November 2022

Aim:
Hepatocellular carcinoma (HCC) is the world's second leading cause of cancerrelated
mortality and the fifth most prevalent cancer overall. Several synthetic and plant-based
remedies are in practice to treat diverse liver disorders. Because of their minimal side effects and
protective characteristics, plant phenolics have the potential to become alternative therapeutics, replacing
currently existing HCC medications. The present study identifies the plant phenolics as
having the capacity to inhibit HCC with low side effects and cost efficiency.

Background:
Hepatocellular carcinoma (HCC) is the leading cause of cancer-related mortality, despite
the proven effectiveness of screening programs for at-risk individuals, the majority of patients
have disease progression or tumor characteristics that preclude curative therapies at the time of diagnosis.
Acteoside (Verbascoside) is a naturally occurring phenylethanoid glycoside found
throughout the plant kingdom. Acteoside is a physiologically active chemical with the number of
pharmacological and protective effects against various liver illnesses.

Objectives:
Currently used HCC medications have a variety of side effects. Plant-based chemicals
offer the possibility of treating HCC with minimal side effects. The work is targeted to find the
best phytochemical (plant phenolic) lead molecule for future drug development research against
Hepatocellular carcinoma.

Methods:
The targets were selected based on an analysis of relevant literature, and the 3D structures
of the selected receptors were obtained in. pdb format from the RCSB-Protein data bank
(PDB, http://www.rscb.org/pdb). Based on a review of the literature, sixty plant secondary metabolites,
or plant phenolics, were selected. The ligand structures were obtained and downloaded in.sdf
format from the NCBI PubChem chemicals database (https://pubchem.ncbi.nlm.nih.gov/). Molecular
docking between the receptor and ligands was accomplished using the Molegro Virtual Docker
6.0 (MVD) software.

Results:
The target RAF1, BRAF chain 1, TIE2 chain 2 FGFR1, FGFR2, AXL, and FGFR4
showed the best binding effectiveness with acteoside compared to their respective positive control.
RET chain 1 and BRAF chain 2 acteoside showed prominent binding efficacy after Curcumin, and
Epigallocatechingallate, respectively, against positive control. Present findings clearly point towards
the potentiality of acteoside in inhibiting various HCC targets.

Conclusion:
Acteoside may be used as a prominent lead molecule in the future treatment of hepatic
cancer with its multifaceted binding efficiencies against various target proteins.
Daya Ovicidal Ekstrak Kulit Buah Muda (Calotropis procera) terhadap Haemonchus contortus secara in vitro
Authors: Wirawan I Gusti Komang Oka , Nurcahyo Wisnu , Prastowo Joko , . Kurniasih 
Published: November 2022

Penelitian ini bertujuan untuk mengetahui konsentrasi efektif ekstrak kulit buah muda Calotropis procera (C. procera) dalam menghambat perkembangan telur cacing Haemonchus contortus secara in-vitro. dari 0,2g/ml sediaan larutan ekstrak stok, albendazole konsentrasi 0,055%. Data hasil penelitian dianalisis secara deskriptif. Konsentrasi efektif ekstrak kulit buah muda C. procera (EKBMCP) dalam menghambat perkembangan daya tetas telur cacing Haemonchus contortus secarain-vitro adalah perlakuan EKBMCP konsentrasi 4,5% dengan daya hambat 88% sedangkan perlakuan EKBMCP konsentrasi 2,5% dan 3,5% daya hambatnya secara berturut-turut adalah 70,5% dan 81%.
Karakterisasi Antibodi Poliklonal terhadap Aflatoksin M1
Authors: Fusvita Angriani , Maryam Romsyah , Pribadi Eko Sugeng 
Published: November 2022

The aim of the research was characterized the polyclonal antibody at AFM for diagnostic reagens on track 1 and measure the levels of AFM in milk. The series of experiments are given a polyclonal antibody testing 1 dimetilaminobenzidin (DAB) by dot blot immunoassay (DBIA) so it will look brown color display on the positive control sample; purification and estimation antibody of AFM concentration with ammonium sulfate; 1 dialysis; fractionation using HiTrap Protein A HP column, measured using a spectrophotometer at a wavelength of 280 nm; and characterized of IgG using SDS-PAGE. DBIA test results showed a typical reaction betweenantigen AFM1-BSA with AFM1-BSA antibody to rabbit serum in the form of brown dots after addition of DAB substrate. The results of spectrophotometric against rabbit serum fractionation showed the type of IgG heavy chain.