bluuebunny/crossref_metadata_2025_split huggingface dataset:
UBXN2A suppresses the Rictor-mTORC2 signaling pathway, an established tumorigenic pathway in human colorectal cancer
Authors: Rezvani Khosrow , Sane Sanam , Sriniva Rekha , Potts Rashaun , Eikanger Morgan , Zagirova Diana , Freeling Jessica , Reihe Casey , Antony Ryan , Gupta Brij , Lynch Douglas , Bleeker Jonathan , Turaihi Hassan , Pillatzki Angela , Zhou Wei , Luo Xu , Agany Diing , Zohim Etienne , Humphrey Lisa , Black Adrian 
Published: July 2022

Abstract
The mTORC2 pathway plays a critical role in promoting tumor progression in human colorectal cancer (CRC). The regulatory mechanisms for this signaling pathway are only partially understood. We previously identified UBXN2A as a novel tumor suppressor protein in CRCs and hypothesized that UBXN2A suppresses the mTORC2 pathway, thereby inhibiting CRC growth and metastasis. We first used murine models to show that haploinsufficiency of UBXN2A significantly increases colon tumorigenesis. Induction of UBXN2A reduces AKT phosphorylation downstream of the mTORC2 pathway, which is essential for a plethora of cellular processes, including cell migration. Meanwhile, mTORC1 activities remain unchanged in the presence of UBXN2A. Mechanistic studies revealed that UBXN2A targets Rictor protein, a key component of the mTORC2 complex, for 26S proteasomal degradation. A set of genetic and pharmacological studies showed that UBXN2A regulates cell proliferation, apoptosis, migration, and colon cancer stem cells (CSCs) in CRC. CRC patients with a high level of UBXN2A have significantly better survival, and high-grade CRC tissues exhibit decreased UBXN2A protein expression. UBXN2A induction in tumor organoids suppresses the mTORC2 pathway. These findings provide new insights into the functions of an ubiquitin-like protein by inhibiting a dominant oncogenic pathway in CRC.
Geometry-driven migration efficiency of minimal cell clusters
Authors: Vercruysse Eléonore , Brückner David , Gómez-González Manuel , Luciano Marine , Kalukula Yohalie , Rossetti Leone , Trepat Xavier , Hannezo Edouard , Gabriele Sylvain 
Published: July 2022

Abstract

The directed migration of epithelial cell collectives through coordinated movements is key to many physiological and pathological processes and is increasingly well understood at the level of large confluent monolayers. Numerous migration processes rely on the migration of small groups of polarized epithelial clusters and their responses to external geometries. However, the interplay of cell-cell interactions and external boundaries in small cell clusters remain poorly understood. To tackle this, we used micropatterned stripes to investigate the migration of small epithelial clusters with well-defined geometries. Here we show that their migration efficiency is strongly dependent on the contact geometry, and in particular whether cell-cell contacts are parallel or perpendicular to the direction of migration. By systematically screening possible interaction mechanisms in a minimal active matter model, we show that a combination of velocity and polarity alignment with contact inhibition of locomotion captures the experimental data, which we then validated via force and intracellular stress measurements, as well as perturbations. Altogether, our results highlight the importance of geometry in defining the migration properties of cell clusters, providing a conceptual framework to extract interaction rules from how active systems interact with physical boundaries.
RNAi-mediated plant sterol modification: a novel strategy for controlling pest caterpillars
Authors: Chen Ivy W. , Grebenok Robert J. , Zhao Cheng , He Li , Lei Jiaxin , Ji Rui , Hernandez Nicolo , Yuan Joshua S. , Koiwa Hisashi , Behmer Spencer T. , Zhu-Salzman Keyan 
Published: July 2022

Abstract
All eukaryotes use sterols as structural components in cell membranes and as precursors for key hormones. However, arthropods are unique among eukaryotes because they are unable to synthesize sterols de novo and must acquire sterols through their diet. Cholesterol is the dominant sterol in most insects, but because plants contain little cholesterol, most insect herbivores convert phytosterols to cholesterol. However, metabolic constraints limit which phytosterols insect herbivores can convert to cholesterol. In this study we used RNA interference to modify the sterol profile of Arabidopsis thaliana to generate phytosterols that would be problematic for insect herbivores. We did this by silencing the Δ8-Δ7-sterol isomerase gene (HYD1) that is responsible for the conversion of Δ8-sterols into Δ7-sterols. Sterol production decreased in Arabidopsis lines with > 95% transcript reduction and half of the total sterol profile in these plants showed a Δ8 configuration. These sterol-modified lines exhibited normal growth, but caterpillars (Plutella xylostella) reared on these plants showed reduced growth and survival, while adults showed decreased reproductive output. Sterol analysis of the pupae reared on sterol-modified plants suggests these negative effects are a function of reduced cholesterol levels and the accumulation of Δ8-sterols. Finally, we used our collective performance and reproduction data to model the effects of sterol-modified plants at the population level. We show that population numbers are significantly reduced, and generation time extended, after only two generations. Our results suggest that modifying plant sterols has real potential for management of pest caterpillars, especially multivoltine species.
The High-Voltage Level Shifter with dV/dt noises Shielding
Authors: Cao Jianwen , Zhou Zekun , Zhang Bo 
Published: July 2022

Abstract
This paper proposes a HV (high-voltage) level shifter to shield the dV/dt noise. The main mechanism is to determine whether there is dV/dt noise through signal changes. When there is the dV/dt noise, the proposed level shifter’s output is locked by the dV/dt noise shielding circuit. So, the proposed level shifter has infinite dV/dt immunity, which is not affected by the supply voltage, and processes. In normal operation, the proposed level shifter can still maintain high response speed by using the auxiliary circuit. Therefore, the proposed HV level shifter cannot only realize dV/dt noise shielding, but also have a fast response speed. In the 0.5µm BCD process, the proposed level shifter is simulated, realizing the ±250V/ns dV/dt noises shielding function and less than 1.5ns delay time under the 400V HV power supply.
Effect of Hybrid Fiber Reinforcements on the Mechanical and Ductility Performance of HPC Incorporating Micro Silica and Alccofine-1203 Prabath, N V N a , and Ramadoss, P b
Authors: N PRABATH N V , P RAMADOSS 
Published: July 2022

Abstract
This study investigates the effect of fiber volume fractions of single and hybrid fibers on the mechanical and ductility performance of high-performance concrete (HPC) with w/b ratios of 0.38 and 0.28, and 20% silica fume replacement. Macro steel fiber volume fractions (Vf = 0.5, 1 & 1.5%) and micro polypropylene fiber volume fractions (Vf = 0.25, 0.5 & 1%) and alccofine-1203 as an additive were used in this study. For high performance fiber reinforced concrete (HPFRC) and high performance hybrid fiber reinforced concrete (HP-HyFRC) mixes, compressive strength, modulus of rupture (MOR) and elastic modulus were evaluated. Test results of HPSFRC show improvement in 28-day compressive strength by 15.4% and in MOR by 44.66% at steel fiber volume fraction, for HPPFRC, improvement in compressive strength and in MOR the improvement is moderate at polypropylene fiber volume fraction, Vf = 1%. Stress-strain behavior of HPFRC and HP-HyFRC was studied and modulus of elasticity was obtained in the range of 33.72-44.26 GPa and 33.72-45.21 GPa, respectively. Engineering properties were improved as a result of synergetic effect between steel and polypropylene fibers. Effect of addition of alccofine-1203 on the mechanical performance of HPC was examined, and the optimum dosage as 8% was observed. HPSFRC improved the compressive toughness and ductility considerably, which is evidenced from stress-stain behavior, and in HP-HyFRC, this improvement is high due to the synergetic effect between both the fibers. Statistical models for the estimation of compressive strength ratios/ flexural strength ratios of HPSFRC/ HPPFRC were developed. The predicted values are in good agreement with the experimental values of earlier researchers. Relationship between 28-day flexural and compressive strengths of HPSFRC/ HPPFRC was developed with AAE= 4.32%. Multiple linear regression model with R= 0.98 was developed by Response surface method for the prediction of compressive strength of HP-HyFRC mixes and validated.
BE-UJI hand function activity set for the evaluation of the healthy and pathological hand
Authors: Jarque-Bou Néstor J. , Gracia-Ibáñez Verónica , Vergara Margarita , Sancho-Bru Joaquín L. 
Published: July 2022

Abstract
Background. Hand kinematics during hand function tests based on the performance of activities of daily living (ADL) can provide objective data to determine patients’ functional loss. However, they are rarely used during clinical evaluation due to their long duration. Starting from the 20 tasks of the Sollerman Hand Function Test (SHFT), we propose to identify a smaller set of ADLs that provides similar kinematic information to the original full set, in terms of synergies, ranges of motion and velocities. Methods. We used an iterative method with the kinematics of 16 joints of the hand while performing the 20 ADLs of the SHFT. For each subject, the ADLs were ordered according to their influence on the synergies obtained by means of principal component analysis, and the minimum number of ADL that represented the original kinematic synergies (maximum angle of 30 degrees between synergies) and keeping the ranges of joint movements (85% of the original ones) were selected for each subject. Then, the set of the most frequently selected ADLs was verified to be representative of the SHFT ADLs in terms of motion strategies, ranges of motion and joint velocities, considering healthy subjects and Hand Osteoarthritis patients.Results. A set of 10 tasks, the BE-UJI activity set, was identified, ensuring a certain bounded synergy similarity (maximum mean angle between synergies of 25.5 degrees), functional joint ranges (maximum differences of 10 degrees) and joint velocities (maximum differences of 15 degrees per second). Tasks obtained are: Pick up coins from purses, lift wooden cubes, pick put nuts and turn them, write with pen, cut with a knife, lift telephone, unscrew lid of jars and pour water from a cup, a jar and a Pure-Pak. These activities warranty the use of the seven most common hand-grips in ADL.Conclusion. The BE-UJI activity set for the hand function assessment could be used in clinics as an alternative to the SHFT, reducing the time of the assessment while allowing clinicians to obtain objective kinematic data of motor strategies, range of motion and joint velocities used by patients.
HIF-1α-induced up-regulated miR-322 forms a feedback loop by targeting Smurf2 and Smad7 to activate Smad3/β-catenin/HIF-1α, thereby improving myocardial ischemia-reperfusion injury
Authors: Dong Wei , Dong Chen , Zhu Jianbing , Zheng Yaofu , Weng Junfei , Liu Leilei , Ruan Yang , Fang Xu , Chen Jin , Liu Wenyu , Peng Xiaoping , Chen Xuanying 
Published: July 2022

Abstract
Background: Myocardial ischemia/reperfusion injury (MIRI) is a major cause of heart failure after myocardial infarction. It has been reported that miR-322 is involved in MIRI progression, while the molecular mechanism of miR-322 in regulating MIRI progression needs to be further probed.Methods: MIRI cell model was established by oxygen‐glucose deprivation/reoxygenation (OGD/R). Cell viability was assessed using MTS assay. Flow cytometry and TUNEL staining were employed to analyze cell apoptosis. In addition, the interactions between miR-322, Smad7/Smurf2, HIF-1α and β-catenin were verified by dual-luciferase reporter gene assay.Results: Our results displayed that miR-322 was significantly downregulated in OGD/R-treated H9c2 cells, and its overexpression resulted in increased cell viability and reduced the apoptosis. Smurf2 and Smad7 were identified as the direct targets of miR-322. Smad7 knockdown or Smurf2 knockdown increased OGD/R-treated H9c2 cell viability and suppressed the apoptosis. Meanwhile, miR-322 mimics abolished the mitigating effect of Smad7 or Smurf2 overexpression on MIRI. In addition, the Smad3/β-catenin pathway was identified as the downstream pathway of Smurf2/Smad7. Moreover, it was found that HIF-1α interacted with the miR-322 promoter, and β-catenin interacted with the HIF-1α promoter to form a loop.Conclusion: HIF-1α-induced up-regulated miR-322 activated the Smad3/β-catenin pathway by targeting Smurf2 and Smad7 to improve MIRI; meanwhile, β-catenin/ HIF-1α formed a positive feedback loop to continuously improve MIRI.
Clinical manifestations and vision-related quality of life in pediatric uveitis
Authors: Li Yiran , Yang Liu 
Published: July 2022

Abstract
Background
To analyze clinical manifestations, visual ability and quality of life in pediatric uveitis and to explore factors affecting visual ability and quality of life.
Methods
This cross-sectional study included 40 patients with pediatric uveitis in the database of Ophthalmology of Peking University First Hospital. All patients completed Cardiff visual ability questionnaire for children (CVAQC) and pediatric quality of life inventory measurement models (PedsQL4.0).
Results
A total of 40 cases (68 eyes) with pediatric uveitis were included in this study. Better visual acuity in better eye was predictive of a lower score of CVAQC, education and distance vision. Better visual acuity in worse eye was predictive of a lower score of CVAQC and distance vision. Better scores of CVAQC was predictive of a lower score of PedsQL4.0, physical health, psychosocial health and school functioning.
Conclusions
Health-related quality of life is related with vision ability in pediatric uveitis.
An intranasal influenza virus-vectored vaccine blocks SARS-CoV-2 replication in respiratory tissues of mice and hamsters
Authors: Deng Shaofeng , Liu Ying , Tam Rachel , Chen Pin , Zhang Jinxia , Mok Bobo , Long Teng , Kukic Anja , Zhou Runhong , Xu Haoran , Song Wenjun , Chan Jasper , To Kelvin Kai-Wang , Chen Zhiwei , Wang Pui , Yuen Kwok-Yung , Chen Honglin 
Published: July 2022

Abstract
We have developed an intranasal vaccine candidate based on a live attenuated influenza virus (LAIV) with a deleted NS1 gene that encodes cell surface expression of the receptor-binding-domain (RBD) of the SARS-CoV-2 spike protein, designated DelNS1-RBD4N-DAF. Immune responses and protection against virus challenge following intranasal administration of DelNS1-RBD4N-DAF vaccines were analyzed in mice and compared with intramuscular injection of the BioNTech BNT162b2 mRNA vaccine in hamsters. DelNS1-RBD4N-DAF LAIVs induced high levels of neutralizing antibodies against various SARS-CoV-2 variants in mice and hamsters and stimulated robust T cell responses in mice. Notably, vaccination with DelNS1-RBD4N-DAF LAIVs, but not BNT162b2 mRNA, blocked replication of SARS-CoV-2 variants, including Delta and Omicron BA.2, in the respiratory tissues of animals. The DelNS1-RBD4N-DAF LAIV system warrants further evaluation in humans for the control of SARS-CoV-2 transmission and, more significantly, for creating dual function vaccines against both influenza and COVID-19 for use in annual vaccination strategies.
Alzheimer’s Disease Risk Factors: APOE ε4 Allele and Traumatic Brain Injuries
Authors: Kim Dohyun 
Published: July 2022

Abstract
Alzheimer's disease (AD) is a neurodegenerative disorder affecting millions of individuals worldwide. Understanding of the risk factors for Alzheimer's is in its preliminary stages, but researchers have recently made several advancements in searching for treatments for this damaging disorder. This paper aims to garner greater insight into two potential risk factors: APOE ε4 allele and Traumatic Brain injuries (TBIs), specifically their leverage on disease diagnosis through metrics measuring hallmarks of the disease: beta-amyloid plaques and neurofibrillary tangles. Data from the Allen Brain Institute was analyzed through statistical tests that included chi-squared, bar charts, stacked bar charts, two-way ANOVAs, and t-tests to deduce these risk factors' mechanisms in impacting Alzheimer's. From the analyses, it could be understood that the APOE ε4 allele is a significant genetic predisposition for this disease, although a causal relationship could not be determined. On the other hand, TBIs were found to play an additive role in the development of Alzheimer's, which was less significant than the APOE ε4 allele's role since there was a higher correlation between TBIs and non-Alzheimer's dementia, specifically neurofibrillary tangle predominant dementia (NFTPD). Analysis of risk factors provides a means for optimizing therapy by identifying and allocating resources more efficiently to those most vulnerable, as well as improved drug delivery through treatments that target biological pathways which initiate specific biomarkers formation.
WITHDRAWN: Effects of different factors on the heat conduction properties of graphene using molecular dynamics and electron microscopy
Authors: Chen Junjie 
Published: July 2022

Abstract
Understanding the physics behind the thermal transport at the nanoscale is critical to continued advances in the development and applications of graphene-based composite materials with enhanced thermal conductivity. However, the thermal transport characteristics of graphene have not yet been fully understood. This study relates to the physical factors limiting heat conduction in graphene. Computational simulations are performed using molecular dynamics to investigate the heat conduction properties of graphene. The effects of grain dimensions and edge states on the thermal conductivity of graphene are investigated. The intrinsic thermal conductivity of graphene is determined. The mechanism of phonon transport in graphene is discussed. The objective of this study is to investigate the effect of grain dimensions and edge states on the heat conduction properties of graphene so as to provide a more comprehensive understanding of the nanoscale thermal transport phenomena occurring within graphene. Particular focus is placed on the physical factors limiting heat conduction in graphene. The results indicated that graphene is of huge interest not only for physics and chemistry but also for other natural sciences. Graphene provides a bridge between materials science and some areas of fundamental physics. Graphene can be considered as a model system for studying two-dimensional physics and chemistry in general. Electrons in graphene have a very high mobility, which means that graphene could possibly be used in electronic applications. Electrons in graphene interact very strongly with photons in the visible light frequencies. Graphene has very high thermal conductivity and, therefore, could be used to remove heat from electronic circuits. Phonon transport within the monolayer graphene ribbon is not fully diffusive, and the thermal transport is dominated by the mechanism of diffusive-ballistic heat conduction. The edge termination states can significantly affect the thermal conductivity. The edge termination states can be tailored to provide desirable thermal properties. The phonon transport properties are enhanced and the probability of phonon-boundary scattering is reduced with substantially smooth edges. Irregularly shaped edges can lead to performance degradation.
Microplastic Fragments from Poultry Entrails in Wet Markets from South Caloocan, Philippines
Authors: Leon Lance Isaac D. , Bautista Isabela Marie R. , Deza Anne Gwyneth M. , Kok Jake Fernando F. , Mundo Erickson F. Del , VinceCruz-Abeledo Chona Camille 
Published: July 2022

Abstract
Microplastics (MPs) have been recorded in different ecosystems, posing the risk of disrupting natural processes in the environment and causing harm to organisms that ingest them. In the Philippines, MPs have been found on various coastlines, freshwater bodies, and both fresh and saltwater creatures. Furthermore, MPs have been observed to interact with human gut microbiota and pose toxic risks in mice guts. With this, the study aimed to examine the occurrence of MPs in the entrails of Gallus gallus domesticus, the domestic chicken, which is a staple food in the Philippines. Gizzards and intestines from samples collected in seven sites across South Caloocan, Philippines were isolated and digested with a 10% potassium hydroxide solution in a 60℃ oven overnight. The resulting filtrates were then scrutinised for microplastics with a compound microscope. Microplastic contamination was found in the intestines of chicken from five sites, and in the gizzards of chicken from three sites. An intensive literature review suggests that microplastics may have entered chickens directly from the food chain or through the contaminated feeds.
Environmental, Social and Governance (ESG): Mapeamento e Análise de Clusters
Authors: Ribeiro Thiago de Luca , Antônio de Lima Anderson 
Published: July 2022

Objetivo: O objetivo deste estudo foi mapear e analisar as principais correntes teóricas de base e de fronteira acerca da pesquisa internacional em Environmental, Social e Governance (ESG). Adicionalmente, também foram analisados quais autores, periódicos e países são mais ativos na pesquisa internacional de ESG.
Método: Foi efetuada uma análise bibliométrica focada em análises de cocitação e de acoplamento bibliográfico, com técnicas de análise fatorial exploratória e mapeamento gráfico através de clusterização. A amostra contemplou 405 artigos publicados até dezembro de 2021 e indexados no banco de dados Web of Science.
Resultados: Através da análise de cocitação, foram identificados 5 correntes de pesquisas, sendo a) Influência das Teorias Organizacionais na ESG; b) Competitividade organizacional; c) Impacto da ESG no desempenho financeiro; d) Responsabilidade Social Corporativa; e e) Teoria dos Stakeholders. Através da análise de pareamento, foram identificadas 6 correntes teóricas de fronteira, sendo: a) investimentos sustentáveis; b) Transparência e conselho administrativo; c) Efeitos da ESG; d) Relações entre desempenho financeiro e desempenho social corporativo; e) Relação entre acionistas e ESG; e f) Riscos e ESG.
Originalidade: Até onde se sabe, este é o estudo bibliométrico mais completo acerca da pesquisa em ESG, contemplando tanto as correntes teóricas de base quanto as de fronteira.
Contribuições teóricas/metodológicas: Foram identificadas neste trabalho 5 correntes teóricas que ajudaram a fundar e desenvolver a pesquisa em ESG como campo científico (histórico) e apontadas 6 correntes teóricas emergentes que ajudam a entender para onde está avançando a pesquisa em ESG na atualidade (fronteira).
Synthesis of Curcumin Derivatives (2.5-Bis((E)-4-Hydroxy-3-Methoxy Benzylidine) Cyclopenta-1-On) from Vanillin and Its Activity Test Against Α-Glucosidase Enzymes
Authors: Martha Rahma Diyan , Danar Danar , Retnosari Rini 
Published: July 2022

Synthesis of curcumin analogs (2.5-Bis((E)-4-Hydroxy-3-Methoxy Benzylidine) Cyclopenta-1-On) based on Claisen-Schmidt condensation using an acid catalyst and inhibition of glucosidase enzyme was carried out. The antidiabetic activity of curcumin analogs was carried out by the use of α-glucosidase enzyme from moldy rice (Oryza sativa). The curcumin analogs were synthesized by reacting the vanillin with cyclopentanone. The structure of all products was confirmed by FTIR, GC-MS, 1H-13C-NMR spectrometers, and the activity enzyme. The results showed the curcumin analogs were yielded in 52.27%, respectively as yellowish-green solid. The inhibition test of the α-glucosidase enzyme showed that the curcumin analogs were the potential to inhibit the α-glucosidase enzyme with an inhibition percentage of about 94.26% in 5 mM.