A new WHO bottle bioassay method to assess the susceptibility of mosquito vectors to public health insecticides: results from a WHO-coordinated multi- centre study
Authors: Corbel Vincent , Kont Mara D. , Ahumada Martha Liliana , Andréo Laura , Bayili Bazoma , BAYILI Koama , Brooke Basil , Caballero Jesús A. Pinto , Lambert Ben C. , Churcher Thomas S. , Duchon Stephane , Etang Josiane , Flores Adriana E. , Gunasekaran Kasinathan , Juntarajumnong Waraporn , Kirby Matt , Davies Rachel , Lees Rosemary Susan , Lenhart Audrey , Lima José Bento Pereira , Martins Ademir J. , Müller Pie , N’Guessan Raphael , Ngufor Corine , Praulins Giorgio , Quinones Martha , Raghavendra Kamaraju , Rus Adanan Che , Samuel Michael , Verma Vaishali , Ying Koou Sin , Sungvornyothin Sungsit , Uragayala Sreehari , Velayudhan Raman , Yadav Rajpal S. 
Published: August 2022

Abstract
Background: The continued spread of insecticide resistance in mosquito vectors of malaria and arboviral diseases may lead to operational failure of insecticide-based interventions if resistance is not monitored and managed efficiently. This study aimed to develop and validate a new WHO glass bottle bioassay method as an alternative to the WHO standard insecticide tube test to monitor mosquito susceptibility to new public health insecticides with particular modes of action, physical properties or both.Methods: A multi-centre study involving 21 laboratories worldwide generated data on the susceptibility of seven mosquito species (i.e. Aedes aegypti, Ae. albopictus, Anopheles gambiae s.s., An. funestus, An. stephensi, An. minimus and An. albimanus) to seven public health insecticides in five classes, including pyrethroids (metofluthrin, prallethrin, and transfluthrin), neonicotinoids (clothianidin), pyrroles (chlorfenapyr), juvenile hormone mimics (pyriproxyfen) and butenolides (flupyradifurone) in glass bottle assays. The data were analysed using a Bayesian binomial model to determine the concentration–response curves for each insecticide–species combination and to assess the within-bioassay variability in the susceptibility endpoints, killing 50% and 99% of the population (LC50 and LC99) or inhibiting oviposition by 50% and 99% (OI50 and OI99) to measure mortality and the sterilizing effect, respectively. Results: Overall, about 200,000 mosquitoes were tested with the new bottle bioassay, and LC50 / LC99 or OI50 / OI99 values were determined for all insecticides. Variation was seen between laboratories in estimates for some mosquito species/insecticide combinations, while other test results were consistent. The variation was generally greater with transfluthrin and flupyradifurone than with other compounds tested, especially against Anopheles species. Overall, the mean within-bioassay variability in mortality and oviposition inhibition were <10% for most mosquito species-insecticide combinations. Conclusion. Our findings, based on the largest susceptibility dataset ever produced on mosquitoes, showed that the WHO bottle bioassay is adequate for evaluating mosquito susceptibility to new and promising public health insecticides currently deployed for vector control. The datasets presented in this study have been used recently by the WHO to establish 17 new insecticide discriminating concentrations (DCs) for either Aedes spp. or Anopheles spp. The bottle bioassay and DCs can now be widely used to monitor baseline insecticide susceptibility of wild populations of vectors of malaria and Aedes-borne diseases worldwide.