bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long Noncoding RNA DLGAP1-AS2 Promotes Tumorigenesis and Metastasis by Regulating the Trim21/ELOA/LHPP Signaling Axis in Colorectal Cancer
Authors: Wang Xue , Zhao Jing , Cheng Han , Li Jiuming , Chen Ying , Cui Kaisa , Tian Lu , Zhang Jia , Li Chaoqun , Sun Shengbai , Feng Yuyang , Yao Surui , Bian Zehua , Huang Shenglin , Fei Bojian , Huang Zhaohui 
Published: January 2022

Abstract
Background: Long noncoding RNAs (lncRNAs) have driven research focused on their effects as oncogenes or tumor suppressors involved in carcinogenesis. However, the functions and mechanisms of most lncRNAs in colorectal cancer (CRC) remain unclear. Methods: The expression of DLGAP1-AS2 was assessed by quantitative RT-PCR in multiple CRC cohorts. The impacts of DLGAP1-AS2 on CRC growth and metastasis were evaluated by a series of in vitro and in vivo assays. Furthermore, the underlying mechanism of DLGAP1-AS2 in CRC was revealed by RNA pull down, RNA immunoprecipitation, RNA sequencing, luciferase assays, chromatin immunoprecipitation, and rescue experiments. Results: We discovered that DLGAP1-AS2promoted CRC tumorigenesis and metastasis by physically interacting with Elongin A (ELOA) and inhibiting its protein stability by promoting tripartite motif containing 21(Trim21)-mediated ubiquitination modification and degradation of ELOA. In particular, we revealed that DLGAP1-AS2decreases phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) expression by inhibiting ELOA-mediated transcriptional activating of LHPP and thus blocking LHPP-dependent suppression of the AKT signaling pathway. In addition, we also demonstrated that DLGAP1-AS2 was bound and stabilized by cleavage and polyadenylation specificity factor (CPSF2) and cleavage stimulation factor (CSTF3). Conclusions: The discovery of DLGAP1-AS2, a promising prognostic biomarker, reveals a new dimension into the molecular pathogenesis of CRC and provides a prospective treatment target for this disease. Keywords: colorectal cancer, DLGAP1-AS2, ELOA, Trim21, LHPP
In Silico Screening and Pharmacological Evaluation of Fluoroquinolones: Lead-Optimization Studies
Authors: Vuppala Srimai , Kim Jaeyoung , Choi Ji Myung , Joo Bo Sun , Jang Joonkyung 
Published: January 2022

Abstract
Fluoroquinolones are broad-spectrum antibiotics regularly used to treat eyes, urinary tracts, and respiratory infections. Transcriptional regulator (TcaR) enzyme plays an important role in the formation of biofilms in Staphylococcus epidermidis. These biofilms are important for the protection of bacteria from the host immune system. By using computer-aided drug design techniques, we investigate the molecular interactions and pharmacological properties of selected fluoroquinolones on Staphylococcus epidermidis TcaR. We identified the hit molecules through molecular docking and pharmacological evaluation of the selected dataset on TcaR. The hit molecules were used as the leads for further optimization studies to design novel pharmacophore analogs against Staphylococcus epidermidis TcaR. The newly designed lead-optimized molecules have shown good binding energies and fitness scores with improved pharmacological properties.
Designing an emotional presence in virtual education
Authors: Jamebozorg Zahra 
Published: January 2022

Abstract
Emotional presence includes emotional response and perception of presence in the virtual learning environment. This study aimed to investigate the components of students' emotional perception and teacher's scheme for emotional response and determine a meaningful relationship between these variables. The quasi-experimental research method was utilized. The statistical population of the study included Tehran University of Medical Sciences students. Questionnaires were used to collect data. The PEYDE questionnaire was used to determine emotional response and perception. The validity and reliability of the questionnaire were estimated. The students were randomly divided into two experimental and two control groups based on the name list. The findings revealed a strong link between teachers' emotional responses and students' perceptions as two dimensions of emotional presence in virtual learning. There is a good fit of the measurement model and compatibility between teachers' and students' perceptions of the virtual learning environment. Therefore, the measurement model in the experimental group has a good fit. The value obtained from the model showed that the value of T is more than 1.96, so the researcher's hypothesis is confirmed in the experimental group at a confidence level of 0.95. Furthermore, because the value obtained Q2 is more than zero and positive, it can be concluded that the model has predictive power. Therefore, focusing on emotional presence is essential for a web-based exploratory community. Teachers' designed emotional response is significantly correlated with students' emotional perception and management in the virtual learning environment.
Managing Peer Support Workers in Swedish Mental Health Services – A Leadership Perspective on Implementation and Sustainability
Authors: Grim Katarina , Bergmark Magnus , Argentzell Elisabeth , Rosenberg David 
Published: January 2022

Abstract
Background: Even though it has been demonstrated that peer support contributes to positive outcomes for service users, organizational implementation issues remain. The aim of the current study was to examine an implementation of peer support from the perspective of managers in order to develop knowledge of factors influencing sustainability of this initiative.Methods: Eighteen managers were interviewed in connection with the introduction of peer support in sixteen mental health settings. The interviews were transcribed and analyzed utilizing inductive as well as deductive approaches where, in the latter, a theoretical framework was applied in order to identify critical implementation components at the levels of organization, team and continuous support. Results: The results suggested that managers were predominantly positive in their evaluation of peer support as a recovery-oriented and user-friendly addition to their services, but noted developmental issues regarding role, professional identity, supervision and financing in relation to other traditional personnel. Managers also reflected on the fact that broader commitment at higher levels of the organizational leadership was largely lacking in relation to implementing user involvement in the organization as a whole. The involvement of the user movement, especially with regard to training and supervision helped prepare staff and support peer workers, yet there was some apprehension attached to the critical scrutiny that this ‘outsider’ perspective might imply. Conclusions: The results confirm previously noted uncertainties regarding peer support as an integrated component of mental health systems. While offering clues as to the factors that would further define and integrate peer support, this study also highlights a number of challenges regarding role, identity and connection to the user movement, that need to be considered by managers before assuming that peer support should be implemented with the same approach as other interventions. The findings suggest that the implementation of peer support requires a comprehensive shift in perspectives, power dynamics and culture. Simultaneously, peer support is highlighted as a catalyst for progress towards increasingly recovery-oriented care-giving cultures.
The Climate Impact of High Seas Shipping
Authors: Wang Shouyang , Li Yuze , Jia Peng , Jiang Shangrong , Li Haijiang , Kuang Haibo , Hong Yongmiao , Zhao Xueting , Guan Dabo 
Published: January 2022

Abstract
Strict carbon emission regulations are set with respect to countries’ territorial seas or exclusive economic zone shipping activities to meet their climate change commitment under the Paris Agreement. However, no shipping carbon mitigation policies are proposed for the world high sea regions, which results in carbon intensive shipping activities on high seas. In this paper, we propose a Geographic-based Emission Estimation Model (GEEM) to estimate shipping GHG emission patterns on high sea regions. The results indicate that annual shipping CO2-e emissions on high seas reach 211.60 million metric tonnes in 2019, accounting for about one-third of all shipping emissions globally and exceeding annual GHG emissions of countries such as Spain. The average emission from shipping activities on high seas is growing at approximately 7.26% per year, which far surpasses the global shipping emission growth rate of 2.23%. We propose the implementation policies on each high seas region with respect to the main emission driver of each high seas identified from our results. Our policy evaluation results show that carbon mitigation policies could reduce 25.46 and 54.36 million tonnes CO2-e in the primary intervention stage and overall intervention stage respectively, with 12.09% and 25.81% reduction rates in comparison to the 2019 annual high seas shipping GHG emission.
Omicron: a chimera of two early SARS-CoV-2 lineages
Authors: Xiong Chenglong , Liu Xiuliang , Xiong Jiasheng , Sun Zhong , Hu Wei , Hu Jingjing , Wang Yuqian , Li Kunyu , Thilakavathy Karuppiah , Chen Mingquan , Zhao Qi , Feng Yi , Jiang Qingwu 
Published: January 2022

Abstract
The current global epidemiology of COVID-19 is now characterized by the emergence and rapid spread of the SARS-CoV-2 Omicron variant on a global scale1,2. Despite the variant’s prompt predominance, there remain knowledge gaps in its origin and evolution history3–6. Here, we show that Omicron lineage SARS-CoV-2 is characterized by the feature of chimera. It was generated by genomic recombination of two early PANGO lineages of SARS-CoV-2. In the recombination event, strains with medium or high circulating intensity like SARS-CoV-2/human/USA/COR-21-434196/2021 belonging to PANGO lineage BA.1 provided the fundamental genome and served as the major parents, while the rare lineage strains like SARS-CoV-2/human/IRN/Ir-3/2019 belonging to B.35, as the minor parents, hybridized their genomic fractions into the major genomes at position 21593-23118nt. This recombination event results in 22 amino acid residue substitutions for the variant of Omicron, including 16 in the pivotal RBD of the spike protein. These substitutions have led to some subtle variations in the spatial structure and the affinity to hACE2 receptor of the spike protein7,8, thereby raising concerns about the effectiveness of available vaccines and antibody therapeutics9–12. The global spread and explosive growth of the SARS-CoV-2 in human population increase opportunities for future recombination13–15.
Gaseous Plasma's Time-behavior on a Plate Motion Damping with Time Influenced by a Non-Uniform Non-Stationary Electric Field.
Authors: Wahid Taha Zakaraia Abdel , Hadouda M. K. 
Published: January 2022

Abstract
The research subject is the kinetic and irreversible thermodynamic behavior of gaseous plasma (GP) flow limited by a moving rigid flat plate (RFP). The effects of an unsteady nonlinear applied electric field (NLAEF) were examined on the GP. To explore gas dynamics with the electron velocity distribution function (EVDF), researchers have concentrated on the Bhatnagar-Gross-Krook (BGK)–model of the kinetic Boltzmann equation (BE). An analytical solution was found using the moment method (MM), traveling wave, and shooting method. As illustrated, mean velocity, shear stress, and electromagnetic fields all founded play essential roles. An interesting comparison between the non-equilibrium EVDF and the equilibrium EVDF is made carefully with 3-Dimensional graphics in various time values. We found that the system goes to an equilibrium state (ES) with time compatible with Le Chatelier's principles. The relations between the various macroscopic variables of the GP are studied. The irreversible non-equilibrium thermodynamics (NT) properties of the system are presented. Entropy and entropy generation are derived, and their behavior is investigated. The essence of entropy, the degree of internal chaos of a system, is gradually described with the advent of statistical physics and information theory. Cybernetics, probability theory, life science, and astrophysics are just a few of the domains where it is functional. According to our findings, NLAEF has a strong effect on GP. Compared to the effect of the nonlinear applied magnetic field (NLAMF), it causes it to vary and disturb substantially. To save the ES for a GP, we should employ NLAMF rather than NLAEF in the GP management procedure. The importance of this research stems from its wide applications in domains such as physics, electrical engineering, micro-electro-mechanical systems (MEMS), and nano-electro-mechanical systems (NEMS) technologies as industrial and commercial sectors.
Practice and associated factors of traditional uvulectomy among caregivers having children less than 5 years old in South Gondar Zone, Amhara Region, Ethiopia, 2020; a triangulated cross-sectional study.
Authors: Yirdaw Berhanu Wale , Gobeza Mengistu Berhanu , Tsegay Netsanet 
Published: January 2022

Abstract
Introduction: Traditional uvulectomy is widely practiced in Africa especially in sub-Saharan countries including Ethiopia. Studies conducted in different times and areas of the world have shown that the level of practice of uvulectomy and its associated factors were varied from country to country. Therefore, this study was carried out to assess the practice and associated factors of traditional uvulectomy among caregivers having children less than 5 years old in the South Gondar Zone.Objective: this study aimed to assess practice and associated factors of traditional uvulectomy among caregivers having children less than 5 years old in South Gondar Zone, Amhara Region, Ethiopia, 2020.Method: a community – based triangulated cross-sectional study was conducted on 644 participants. Quantitative data were collected from 634 participants using a structured interviewer-administered Amharic version questionnaire; it was entered into Epi Data and analyzed using SPSS. Qualitative data were collected from 10 participants via an in-depth interview and analyzed by OpenCode. Descriptive statistics were calculated and logistic regressions were fitted to declare statistical significance at p-value < 0.05 and 95% CI.Result: the prevalence of traditional uvulectomy in this study was 52.5% (95% CI, 48.6 – 56.3%). Moreover, lack of information [AOR = 2.975 (1.677 - 5.277)], perceived as uvula causes illness [AOR = 4.888 (2.954 - 8.086)], future intention or will perform [AOR = 4.188 (2.584 - 6.788)], perceived as traditional uvulectomy should not be eradicated [AOR = 1.893 (1.172 - 3.057)]), saw the previous good result [AOR = 9.396 (5.512 - 16.016)], health personnel hospitality problem [AOR = 5.922 (2.392 – 14.664)] and did not get cured by pharmacologic treatment [AOR = 3.918 (2.073, 7.405)] were significantly associated with traditional uvulectomy.Conclusion and recommendation: the prevalence of traditional uvulectomy was high. Lack of information, perceived as uvula causes illness, future intention to uvulectomy, perceived as traditional uvulectomy should not be eradicated, saw the previous good result, health personnel hospitality problem and did not get cured by pharmacologic treatment were the factors significantly associated with traditional uvula cutting. Therefore, special attention will be given to creating further awareness to the community at large and setting controlling mechanisms for the health care delivery system.
Determinants of neonatal near miss among neonates delivered in public hospitals in Shashemane city administration, Ethiopia, 2021 Facility based unmatched case control study
Authors: Guda Henok Girma 
Published: January 2022

Abstract
Background: Neonatal near miss is an event that nearly died between 0-28 days but survived by chance or good quality of care. The number of neonates who survived morbidities were approximately 5 times greater than those died. However, there is limited evidence that state the determinants of neonatal near miss in Ethiopia and particularly in Shashemane. So, this study attempted to identify determinants of Neonatal near miss among neonate delivered in public Hospitals in Shashemene, Oromia, Ethiopia.Methods: Facility based unmatched case-control study conducted from March, 22, 2021 to May 22, 2021. Structured and pretested questionnaires used for data collection. 104 cases were selected consecutively and 208 controls were selected by systematic random sampling by 2nd k during study period. For each near-miss case, two controls were selected. After data collection, data were checked for consistency, coded and entered by using EPI INFO 7 and exported to statistical package for social science for analysis by using binary logistic regression on the basis Odds ratio, 95% CI and p-value of less than 0.05. Variables with p < 0.25 in Bivariable analysis entered into a multivariable logistic regression model using the backward variable selection method.Result: The result show that mother age group between 20-34 had 0.12 less odds to experience neonatal near miss than age group below 20 years old. [AOR=0.12, 95%(CI=0.02-0.76)]. Neonates who were delivered by spontaneous vaginal delivery had 0.38 less odds of experiencing neonatal near miss than neonates delivered by instrumental assisted delivery and Cesarean section [AOR 0.38, 95% (CI= 0.22-0.68)]. Delivery followed by partograph had 0.25 less odds likely to develop neonatal near miss than those not followed by partograph [AOR=0.25 95% (CI,0.11-0.54)].Conclusion: Age of mother, gestational age, delivery mode and delivery followed by partograph were determinants of the neonatal near miss. So, women conceive at below 20 years of old, mother that will not give birth by spontaneous vaginal delivery should be advised of the greater risk of neonatal near miss. Health worker in delivery ward should use partograph for every delivery in both hospitals.
Research progress on meibomian gland dysfunction from 2011 to 2020: a bibliometric and visualized analysis
Authors: Liu Qian 
Published: January 2022

Abstract
Background
To explore the research areas, hotspots, and progress of meibomian gland dysfunction through bibliometrics.
Methods
Related publications were retrieved from the Web of Science Core Collection from 2011 to 2020. VOSviewer1.6.16, Citespace.5.7.R2, and GraphPad Prism 8 were used to visualize the distribution of countries, research institutions, journals, authors, keywords, and annual publication numbers in this field.
Results
A total number of 716 relevant publications were retrieved. The United States and Keio University ranked the first among the countries and organizations with the most publications. Cornea, Investigative Ophthalmology & Visual Science, and Ocular Surface were the top three journals with the highest publication counts and citations. The authors who contributed to this topic mainly formed three clusters which manifested the research areas, and the extracted keywords mainly formed four clusters which manifested the hotspots were explored.
Conclusions
The research areas and hotspots of meibomian gland dysfunction were as follow: (1) Pathogenesis or potential etiology of meibomian gland dysfunction; (2) Diagnosis of meibomian gland dysfunction; (3) Therapy of meibomian gland dysfunction and the International Workshop`s dedication to it; (4) Epidemiology of meibomian gland dysfunction.
Treatment of calcified lumbar disc herniation by intervertebral foramen remolding a retrospective study
Authors: Chen bin , yuan ao lin , shen xin 
Published: January 2022

Abstract
Background： Percutaneous endoscopic lumbar discectomy is a new treatment method for lumbar disorders but remains controversial, similar to other minimally-invasive treatments for calcified lumbar discs. This study aimed to investigate the use of the percutaneous intervertebral foramen lens technology for secondary molding of the intervertebral foramen in the treatment of calcified lumbar discs.Methods： The study included 104 patients who were divided into two groups. Group A comprised 50 patients (age: 49.9±14.5 years, 30 [60%] females) with calcified lumbar disc herniation and group B comprised 54 patients (age: 53.2±15.3 years, 32 (59%) females) with non-calcified lumbar disc herniation diagnosed by computed tomography and magnetic resonance imaging. Patients underwent a percutaneous endoscopic lumbar discectomy at our hospital from January 1, 2017, to December 31, 2019. Demographic characteristics before the surgery and perioperative outcomes were retrospectively reviewed. The treatment outcome was analyzed using the numerical rating scale (NRS) score, Oswestry Disability Index (ODI) score, and modified Macnab criteria. Results： Patients in groups A and B showed significant improvement in both the NRS and ODI scores after the surgery and maintained relatively low ODI and NRS scores during subsequent follow-ups. In group A, 94% of patients rated the results as “excellent” or “good” according to the modified Macnab criteria at the 3-month follow-up. One patient developed neck pain during the surgery, which was diagnosed as spinal hypertension syndrome, and the surgery was suspended until the patient’s condition improved. In group B, 92.6% of patients rated the results as “excellent” or “good” according to the modified Macnab criteria at the 3-month follow-up. In both the groups, no patient reported any dural leak, infection, or other related complications.Conclusions: Our results indicate that transforaminal remolding is effective in the treatment of non-calcified lumbar disc herniations, with few intraoperative and postoperative complications and that secondary reconstruction of the intervertebral foramen under visual conditions using microscopic knife is an effective method for treating calcified lumbar disc herniation, with few intraoperative and postoperative complications.
Development of the T-ALLiPSC-based Therapeutic Cancer Vaccines for T-cell Acute Lymphoblastic Leukemia
Authors: Liu Tao , Li Zhu , Chen Xuemei , Liu Luning , Zhou Meiling , Zhou Guangqian 
Published: January 2022

Abstract
The T-cell acute lymphoblastic leukemia (T-ALL) is a kind of hematological malignancy in children. Despite the significant improvement in the cure rate of T-ALL upon treatment with chemotherapy regimens, steroids, and allotransplantation there are relapses. This study focuses on the tumor-specific therapeutic vaccines derived from the induced pluripotent stem cells (iPSC) to address the issue of T-ALL recurrence. Patient-derived tumor cells and healthy donor cells were reprogrammed into the iPSCs and the RNA-seq data of the T-ALL-iPSCs and H-iPSCs were analyzed. In vitro, the whole cell lysate antigens of iPSCs were prepared to induce the dendritic cells (DC) maturation, which in turn stimulated the tumor-specific T cells to kill the T-ALL tumor cells (Jurkat, CCRF-CEM, MOLT-4). The cytotoxic T lymphocyte (CTL) stimulated by the DC-loaded T-ALL-iPSC-derived antigens showed specific cytotoxicity against the T-ALL cells in vitro. In conclusion, the T-ALL-iPSC-based therapeutic cancer vaccine can elicit a specific anti-tumor effect on T-ALL.