bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of 25-hydroxyvitamin D (25(OH)D) levels before and during the COVID-19 pandemic: a cross-sectional study and trend analysis involving 86,772 samples
Authors: Arayici Mehmet Emin , Uysal Sila Ovgu Korkut , Leblebici Asim , Akcali Zeynep , Edizer Deniz Tuna , Kabul Seher , Cimrin Dilek , Ellidokuz Ender Berat 
Published: November 2022

Abstract
Purpose
The aim of this paper was to evaluate the change in 25-hidroxyvitamin D (25(OH)D) levels before and during the COVID-19 pandemic.
Methods
In this retrospective, cross-sectional and methodological study included 86,772 patients (18–75 years) samples who were admitted to the University Hospital for various reasons and whose 25(OH)D levels were measured in the biochemistry unit between 2019–2020 and 2020–2021 (before and during the COVID-19 outbreak). A time series analysis of monthly averages for 25(OH)D was performed. For the purpose of seasonal study, the mean levels of 25(OH)D are grouped by years. Data were modeled in terms of 25(OH)D levels using the MATLAB Curve Fitting Toolbox.
Results
There was no significant difference between the sexes according to 25(OH)D levels (p > .05). 25(OH)D levels were significantly higher in the summer months and lower in the winter months (p < .001). When comparing the spring months, 25(OH)D levels in 2020 were found to be significantly lower than in 2019 (p < .001); on the contrary, when examined based on the summer, autumn, and winter months, 25(OH)D levels increased in 2020 compared to 2019 (p < .001). In the estimates curve obtained with an error margin of 11% in the time series analysis, it was estimated that the 25(OH)D averages after the pandemic would be similar to those before the pandemic.
Conclusion
Restrictions, partial or complete closures, and curfews can significantly affect individuals' 25(OH)D levels during the COVID-19 outbreak. Evidence-based public health policies regarding vitamin D, especially during pandemic and closure periods, have critical importance.
The level of headache disability among different population samples in Kuwait and its relationship to cognitive distortions
Authors: Haggag Abdelmotaleb Abdelkader 
Published: November 2022

Abstract
Headache disorder is spreading at a high rate all over the world. And it is not only painful, but also disabling. Headache disorders impose a significant burden on patients including significant personal suffering, deterioration in quality of life, financial cost, and damage to family life, social life and employment. This research aimed to know the level of headache disability among different population samples in Kuwait and its relationship to cognitive distortions. An Internet-based cross-sectional study was conducted where the participants from different population samples in Kuwait were targeted using total population sampling. 450 participants from Kuwaiti society were recruited, between September4 and October28, 2022. Participants completed two self-reported scales. The scales included The Henry Ford Hospital headache disability inventory (HDI):and The Cognitive Distortions Questionnaire (CD-Quest). The results reveal that there is low level of headache disability among participants. There were differences between participants with high headache disability and participants with low headache disability in the total score of CD-Quest and its dimensions. There was a positive correlation between headache disability and the total score of CD-Quest and most of its dimensions. The groups most suffering from headache disability were: high -income peoples, residents, participants aged 25-40 years, females, marrieds, patients with chronic diseases, participants with post-graduate qualifications.
COVID-19 related changes in eating disorder pathology, emotional and binge eating and need for care: a systematic review with frequentist and Bayesian meta-analyses
Authors: Molendijk Marc , Guzel Âmine , Mutlu Naz Lâl 
Published: November 2022

Abstract
Objective
The COVID-19 pandemic has been a leading cause of stress and feelings of loss of control, both of which have been related to Eating Disorder (ED) pathology onset and symptom deterioration. To estimate the magnitude of changes in the prevalence rates of, and indicators for, ED psychopathology in the face of the COVID-19 pandemic.
Method
Pre-registered systematic review with frequentist and Bayesian meta-analyses. Searches for eligible studies were performed in PubMed, Web of Science and pre-print servers until March 18 2022.
Results
Our searches yielded 39 eligible studies on a total of 4.639.947 subjects. Rates of diagnosed and self-reported ED’s and ED symptoms significantly increased in the face of the pandemic. ED symptoms and severity, and emotional and binge eating were reported more often during the pandemic. The need for ED related care increased concordantly. The strength of significant associations was small-to-moderate in most cases. Findings of frequentist and Bayesian meta-analysis converged. Moderators of the substantial between-study heterogeneity were not detected and there was little evidence for the existence of publication bias.
Conclusions
Altogether, our results suggest that the COVID-19 pandemic is associated with a wide spread negative effect on ED pathology. The development of online prevention and intervention programs for EDs during stressful times like a pandemic is encouraged. A limitation is that results may be prone to biases, amongst others, self-report bias.
Preregistration
Prospero [https://www.crd.york.ac.uk/prospero] ID: CRD42022316105
Evaluating the efficacy of human dental pulp stem cells and scaffold combination for bone regeneration in animal models: A systematic review and meta-analysis
Authors: Namjoynik Amin , Islam Md Asiful , Islam Mohammad 
Published: November 2022

Abstract
Introduction:
Human adult dental pulp stem cells (hDPSC) and stem cells from human exfoliated deciduous teeth (SHED) hold promise in bone regeneration for their easy accessibility, high proliferation rate, self-renewal and osteogenic differentiation capacity. Various organic and inorganic scaffold materials were pre-seeded with human dental pulp stem cells in animals with promising outcomes in new bone formation. Yet, the clinical trial for bone regeneration using dental pulp stem cells is still in infancy. Thus, the aim of this systematic review and meta-analysis is to synthesise the evidence of the efficacy of human dental pulp stem cells and the scaffold combination for bone regeneration in animal bone defect models.
Methodology:
This study was registered in PROSPERO (CRD2021274976) and PRISMA guideline was followed to include the relevant full-text papers using exclusion and inclusion criteria. Data were extracted for the systematic review. Quality assessment and the risk of bias of the included papers were also carried out using CAMARADES tool. Quantitative bone regeneration data of the experimental (Scaffold + hDPSC/SHED) and the control (Scaffold only) groups were also extracted for meta-analysis.
Results
49 papers were included for systematic review and only 27 of them were qualified for meta-analysis. 90% of the included papers were assessed as medium to low risk. In the meta-analysis, qualified studies were grouped by the unit of bone regeneration measurement. Overall, bone regeneration was significantly higher (p < 0.0001) in experimental group (scaffold + hDPSC/SHED) compared to the control group (scaffold only) (SMD: 1.863, 95% CI: 1.121–2.605). However, the effect is almost entirely driven by the % bone formation group (SMD: 3.929, 95% CI: 2.612–5.246) while % BV/TV (SMD: 2.693, 95% CI: -0.001-5.388) shows a marginal effect and both bone mineral density (mg/cm3) and new bone formation (mm2) showed no effects. The funnel plot exhibits no obvious asymmetry representing a lack of remarkable publication bias. Sensitivity analysis also indicated that the results generated in this meta-analysis are robust and reliable.
Conclusion
This is the first synthesised evidence that showed that irrespective of the types or composition of the scaffolds, DPSCs/SHED and scaffold combination enhanced bone regeneration highly significantly compared to the cell-free scaffold. So, dental pulp stem cells would be a promising tool for treating various bone diseases, and more clinical trials need to be conducted to evaluate the effectiveness of dental pulp stem cell-based therapies.
Application of neural network approach for modelling COD reduction from real refinery effluent by electrocoagulation
Authors: Madi Nor el houda , Chabani Malika , Bouafia-Chergui Souad , Zier Taha , Rechidi Youcef 
Published: November 2022

AbstractThe present study aims to investigate the feasibility of implementing the electrocoagulation (EC) process to treat Algiers refinery effluent. The electrocoagulation was performed by using scrap aluminum plate electrodes in monopolar-parallel mode. Several parameters, namely current density, reaction time, the electrolyte dose, and the initial chemical oxygen demand (COD) concentration were studied. The maximum removal of COD achieved was found to be 78.55%. Operating conditions at which maximum COD removal efficiencies were achieved at current density 8 mA/cm2, electrolyte dose 1 g/L, with 360 mg/L of initial COD concentration at working time of 40 min. An artificial neural network (ANN) was also utilized to determine predicted responses using neural networks for the 4-10-1 arrangement. The responses predicted by ANN were in alignment with the experimental results. The values of the determination coefficient (R2 = 0.978) and the root mean square error (RMSE = 21.28) showed good prediction results between the model and experimental data. Hence, the ANN model as a predictive tool has a great capacity to estimate the effect of operational parameters on the electrocoagulation process.
Convenient Synthetic Approaches for Novel Impurities of Propisochlor
Herbicide
Authors: Mohanta Prajna Parimita , Pati Hari Narayan , Behera Ajaya Kumar 
Published: November 2022

Abstract:
A facile and convenient synthesis of two new and one known impurities of Propisochlor,
2-chloro-N-(2-chloroethanoyl)-N-(2-ethyl-6-methylphenyl)-ethanamide, 2,2-dichloro-N-(2-
ethyl-6-methyl-phenyl)-N-(isopropxymethyl)-ethanamide and 2-chloro-N-(2-ethyl-6-methylphenyl)-
N-methylethanamide have been developed from the readily available 2-ethyl-6-
methylaniline precursor. All the synthesized impurities are characterized using 1H, 13C NMR and
mass spectra for their structural elucidation.
Fifty Years of Motor Vehicle Crashes in Saudi Arabia: A Way Forward
Authors: Bendak Salaheddine , Al-Shammari Naif , Kim In-Ju 
Published: November 2022

Introduction:This study involves an in-depth assessment of the state of traffic safety across the Kingdom of Saudi Arabia over the last fifty years.Methods:A comprehensive dataset of 59 published peer-reviewed journal articles, as well as 212 government reports and official statistics, was collated and critically assessed to determine major factors affecting traffic safety in the country.Results and Discussion:Results reveal that some traffic safety indicators are still showing worsening trends despite the great efforts to improve road safety in Saudi Arabia, while few other areas have either stabilized or started showing some improvements over recent years. Findings also show that human losses (estimated to be 4.7% among all mortalities) and economic losses (estimated to be as high as $16b annually) are significantly higher than developed and most developing countries. Contributing factors to the high losses include risky behaviors of drivers and road users, speeding, unrestrained seatbelts, use of handheld electronic devices and/or mobile phones, high motor vehicle ownership rate, and others.Conclusion:It can be concluded that various traffic safety indicators have been giving mixed signs in Saudi Arabia over the last fifty years, and a change in safety behavior among road users is needed along with new strategies to minimize motor vehicle crashes.
Paliperidone-benzamide Cocrystals: Preparation, Characterization, In
Vitro/In Vivo Evaluation
Authors: Rani Earle Radha , Radha Gadela Venkata 
Published: November 2022

Background:
The current investigation contributes to the development of novel Paliperidone
(PPD) co-crystals (CCs) using benzamide (BZ) as a conformer. The CCs were synthesized using the solvent
evaporation technique.

Methods:
The enhancement in solubility was studied by saturation solubility studies. Structural characterization
of CCs was performed by Fourier Transform Infra-Red Spectroscopy (FTIR), powder X-ray diffraction
(PXRD), Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM) and
Proton Nuclear Magnetic Resonance (1H- FT NMR) to verify CC formation.

Results:
CCs exhibited a higher aqueous solubility of 2.067±0.004mg/ml when compared to pure drug
0.473±0.012mg/ml. This designated aqueous solubility enhancement of CCs by 4.36 folds. In vitro dissolution
data of the CCs exhibited a drug release of 96.5±1.63% in 60min, while pure drug showed a poor
release of 37.8±1.76% in the same time period In vivo studies resulted in enhanced rate and extent of drug
absorption from CCs when compared to drug suspension.

Conclusion:
CCs formed between PPD and BZ present a novel approach in overcoming the hurdles in the
solubility of PPD that exhibits poor aqueous solubility.
A Cost-effectiveness Analysis of a Mobile Phone–Based Integrated HIV-Prevention Intervention Among Men Who Have Sex With Men in China: Economic Evaluation
Authors: Yun Ke , Yu Jiaming , Liu Changyang , Zhang Xinxin 
Published: November 2022

Background
Mobile phone–based digital interventions have been shown to be a promising strategy for HIV prevention among men who have sex with men (MSM).

Objective
This study aimed to evaluate the cost-effectiveness of a mobile phone–based digital intervention for HIV prevention among MSM in China from the perspective of a public health provider.

Methods
The cost-effectiveness of the mobile phone–based digital intervention was estimated for a hypothetical cohort of 10,000 HIV-negative MSM who were followed for 1 year. A model was developed with China-specific data to project the clinical impact and cost-effectiveness of two mobile phone–based digital strategies for HIV prevention among MSM. The intervention group received an integrated behavioral intervention that included 1) individualized HIV infection risk assessment, 2) recommendation of centers testing for HIV and other STIs, 3) free online order of condoms and HIV and syphilis self-test kits and 4) educational materials about HIV/AIDS. The control group was only given educational materials about HIV/AIDS. Outcomes of interest were the number of HIV infections among MSM averted by the intervention, intervention costs, cost per HIV infection averted by the mobile phone–based digital intervention, and quality-adjusted life-years (QALYs). Univariate and multivariate sensitivity analyses were also conducted to examine the robustness of the results.

Results
It is estimated that the intervention can prevent 48 MSM from becoming infected with HIV and can save 480 QALYs. The cost of preventing 1 case of HIV infection was US $2599.87, and the cost-utility ratio was less than 0. Sensitivity analysis showed that the cost-effectiveness of the mobile phone–based digital intervention was mainly impacted by the average number of sexual behaviors with each sexual partner. Additionally, the higher the HIV prevalence among MSM, the greater the benefit of the intervention.

Conclusions
Mobile phone–based digital interventions are a cost-effective HIV-prevention strategy for MSM and could be considered for promotion and application among high-risk MSM subgroups.
Karakterisasi Staphylococcus aureus Isolat Susu Sapi Perah Berdasar Keberadaan Protein-A pada Media Serum Soft Agar terhadap Aktivitas Fagositosis Secara In Vitro
Authors: Lestari Fajar Budi , Salasia Siti Isrina Oktavia 
Published: November 2022

Staphylococcus aureus merupakan salah satu bakteri penyebab utama mastitis. Protein-A berperan penting dalam adesi dan kolonisasi bakteri pada sel inang. Penelitian ini bertujuan untuk mengetahui hubungan aktivitas fagositosis S. aureus berdasarkan keberadaan protein-A pada media serum soft agar. Sebanyak 19 isolat S. aureus susu sapi perah asal Jawa Barat dan Jawa Tengah digunakan pada penelitian ini. Seluruh isolat tersebut direidentifikasi dengan dipupuk pada media plat agar darah (PAD), koloni bakteri kemudian diidentifikasi dengan pewarnaan Gram, uji mannitol salt agar (MSA), katalase dan uji koagulase. Karakterisasi S.aureusdilakukan dengan menanam bakteri pada media serum soft agar (SSA) yang mengandung serum kelinci untuk mengetahui keberadaan protein-A, kemudian dilakukan uji fagositosis dengan menggunakan sel polimorfonuklear. Dari 19 isolat tersebut seluruhnya teridentifikasi sebagai S. aureus yang ditunjukkan dengan Gram positif, sel berbentuk kokus bergerombol, mampu memfermentasi manitol pada media MSA, positif pada uji katalase, 15,79% sampel menunjukkan hasil koagulase negatif, sedangkan 84,21% menunjukkan hasil koagulase positif. Pertumbuhan pada media SSA menunjukkan hasil 12 isolat (63,16%) koloni berbentukkompak dan 7 isolat (36,84%) koloni berbentuk difus. Koloni kompak menunjukkan bakteri tersebut memiliki protein-A, koloni difus menunjukkan bakteri tersebut tidak memiliki protein-A atau memiliki protein-A tetapi tertutup oleh kapsul. Hasil uji fagositosis menunjukkan S. aureus yang memiliki protein-A lebih sedikit difagosit oleh leukosit polimorfonuklear (2,99 bakteri/sel) dari pada S. aureus yang tidak memiliki protein-A, atau mempunyai protein-A tetapi tertutup oleh kapsul (3,85 bakteri/sel). Staphylococcus aureus yang memiliki protein-A lebih patogen daripada S. aureus yang tidak memiliki protein-A. Isolat S. aureus asal Jawa Tengah lebih virulen dibandingkan isolat S. aureus asal Jawa Barat ditinjau dari sifat hemolisis, koagulase, dan protein-A.
Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer
Authors: Kristianingrum Yuli Purwandari , Sitarina Widyarini Sitarina Widyarini , Kurniasih Kurniasih , Bambang Sutrisno Bambang Sutrisno , Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu , Sugiyono Sugiyono 
Published: November 2022

Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected bytrimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brainwere observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell deathbetween control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day aftertreatment, especially in the hippocampus and cortex.
Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat
Authors: Hafid Muhammad Dusil , Anshary Hilal 
Published: November 2022

Anisakis (L3-stage) is a parasitic nematode commonly found in marine fish or squid serve as intermediate or paratenic host. The purpose of this research was to identify Anisakis larvae using PCR-RFLP and sequencing methods. A total of 30 individuals of each species: bullet tuna (Auxis rochei) and Indian scad(Decapterus russeli) were examined for presence of Anisakis spp. Fish samples caught by fishermen around Mamuju waters were purchased at a fish market. The fish was necropsied and the organs: liver, intestine wall, heart and muscle were removed and put on separated petri dishes and examined for parasites under a stereo microscope. Anisakis found was cleaned and fixed in 70% alcohol. Initial identification was based on ventriculus shape and presence of mucron to distinguish Anisakis type I and Anisakis type II. All Anisakis found belong to Anisakis type I. Ten Anisakis type I were isolated, cleaned and stored in microfuge tubes containing 70% alcohol. The parasite gDNA was extracted using Wizard genomic DNA extraction and purification kit (Promega).Species of Anisakis was determined by PCR-RFLP at ITS1-5.8S-ITS2 region using two restriction enzymes: Taq I and Hinf I and PCR-sequencing in mtDNA cox2 region. Analysis by PCR-RFLP showed that all Anisakis type Iexamined was in the same pattern as Anisakis typica. Sequencing in mtDNAcox-2 region and the phylogenetic analysis showed that all samples were in the same cluster as A. typica. Based on PCR-RFLP and sequencinganalysis, all Anisakis found in this study belong to A. typica.
Identifikasi Virus Avian Leukosis Sub Grup J pada Peternakan Ayam Petelur Komersial di Kabupaten Tangerang Tahun 2015
Authors: Hartawan Risza , Dharmayanti Ni Luh Putu Indi 
Published: November 2022

Avian leukosis virus (ALV) is one of causing agents for neoplastic syndrome in commercial chicken farms. There are six sub groups of ALV in chicken including A, B, C, D and E. However, the sub group J (ALV-J) is the most alarmed and anticipated because of its severe economic repercussion. The objective of this study was to identify the presence of ALV-J in the commercial chicken farms in Tangerang District. Two identification methods were utilized including antigen capture enzyme-linked immunosorbent assay (ac-ELISA) technique and nested reverse transcriptase polymerase chain reaction (nRT-PCR) test. As results, the ac-ELISA test detected that most of 150 serum samples from 5 commercial layer farms were positive for ALV in about 86,67%. On the other hand, none of the 60 cloacal swab and 60 albumen samples was positive for presence ALV. Subsequently, testing of 45 serum samples from 5 farms using nRT-PCR showed that in about 20% of these samples were positive of ALV-J. These evidences indicated the circulation of ALV-J with other sub groups in thecommercial layer farms in Tangerang District.