bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mir-23b down-regulates the expression of target gene of acetaldehyde dehydrogenase 1a1 and increases the sensitivity of cervical cancer stem cells to cisplatin
Authors: Tao Yongbing , Mao Fuyun , Gu Weihong , Wu Ling , Guo Jing , Xu Ping 
Published: January 2022

Purpose: To study the effect of miR-23b on the expression of the target gene of acetaldehyde dehydrogenase 1A1 (ALDH1A1), and cisplatin (CDDP) susceptibility of cervical carcinoma stem cells.
Methods: Human cervical cancer cell line Hela cells were cultured in vitro, and miR-23b mimic and negative control were transfected into the cells using lipofectamine method. The growth of the two groups of cells was determined using growth curve method, and their proliferation measured using plate clone formation. The influence of treatments on the sensitivity of the cells to CDDP was assayed using MTT method. The mRNA expression of ALDH1A1 in Hela cells was assayed using real-time quantitative polymerase hain reation (PCR), while its protein expression was assayed by Western blot.
Results: The levels of expressions of ALDH1A1 protein and mRNA in the miR-23b overexpression group were significantly lower than those in the control group (p < 0.05). The sensitivities of Hela cells to CDDP in the ALDH1A1 inhibition group and the control group were dose-dependent to some extent, but cell inhibition in ALDH1A1 inhibition group markedly increased, relative to control when the CDDP dose was 0.1 ppc (p < 0.01).
Conclusion: Up-regulating the expression of miR-23b significantly inhibits the growth and proliferation of cervical cancer cells, and increases their sensitivity to CDDP via down-regulation of the expression of the target gene for ALDH1A1. Therefore, during cervical carcinoma treatment, increasing the level of miR-23b may produce a chemotherapeutic effect.
Keywords: MiR-23b, Acetaldehyde dehydrogenase 1A1, Cervical cancer, Cisplatin, Sensitivity
Serum Sestrin2 and PlGF levels in pregnant women with pre-eclampsia and their correlations with the severity of the disease: A case-control study
Authors: Li Lijun , Sun Zhen , Liu Shasha , Cui Hongyan 
Published: January 2022

Purpose: Sestrin2, a metabolic regulator with antioxidant activity, might have some certain predictability for the occurrence and severity of pre-eclampsia (PE). The purpose of this work was to explore the levels of serum Sestrin2 and PlGF in pregnant women with PE and their correlation with the severity index of the disease.Methods:This was a retrospective case-control study of pregnant women with PE who planned to give birth in our hospital from 05/2017 to 05/2019. Pearson correlation was used to analyze Sestrin2 and PlGF levels with PE severity. Receiver operating characteristic (ROC) curves were used to evaluate the predictive value of Sestrin2 and PlGF for PE.Results: There were 52 women in the control group, 46 in the mild PE group, and 36 in the severe PE group. As the severity of PE increased, the median levels of Sestrin2 increased (8.1, 9.8, and 11.8 ng/ml), and the levels of PlGF decreased (156, 117, and 67 pg/ml) in the three groups of women (all P<0.05).The levels of Sestrin2 and PlGF were strongly correlated with mean arterial pressure,proteinuria, newborn birth weight and gestational weeks at delivery (all P<0.001). Sestrin2 and PlGF had high efficiency of diagnosing PE (cut-off: 8.90 ng/ml, Area Under Curve [AUC]=0.979; cut-off: 122.50 pg/ml, AUC=0.963). Additionally, Sestrin2 and PlGF showed high value of severity prediction (cut-off: 11.15 ng/ml, AUC=0.857; cut-off: 68.50 pg/ml, AUC=0.837).Conclusion: Sestrin2 and PlGF are correlated with the severity of PE. Both Sestrin2 and PlGF had high value for PE diagnosis and severity prediction.
Keywords: pre-eclampsia; Sestrin2; placental growth factor; oxidative stress; severity
Studies of the pharmacokinetics of morroniside and loganin in rat after oral administration of raw and wine-processed Corni fructus by UPLC-QqQ-MS/MS
Authors: Wang Wei , Wang Xuechun , Zhang Qiang , Jia Ru , Du Chunjie , Ju Chengguo 
Published: January 2022

Purpose: To study the pharmacokinetics of morroniside (MR) and loganin (LG) in rats after oral administration of raw and wine-processed Corni fructus by UPLC-QqQ-MS/MS.
Methods: Arctiin (AT) was used as internal standard, and the plasma or tissue samples were extracted twice using ethyl acetate. Electrospray ionization (ESI) negative ion mode was used, and the multiple reaction monitoring mode (MRM) was set in acquisition mode. The extraction and detection method is supported by selectivity, sensitivity, precision, accuracy, stability, extraction, recovery and matrix effect. The non-atrioventricular model of das2.0 software was used to calculate the pharmacokinetic parameters.
Results: The absorption rate of MR (PTmax=0.092) and LG (PTmax=0.092) in Corni Fructus after wine-processing was faster in rats. The mean residence time was longer, and distribution of MR (PMRT0~t = 0.294) and LG (PMRT0~t = 0.000) in wine-processed Corni Fructus group increased in liver and kidneys.
Conclusion: The proposed method has been successfully validated and is suitable for studying the pharmacokinetics of the two analytes in rats.
Anticancer effect of 7-hydroxycoumarin in cisplatin-resistant ovarian cancer cell is mediated via apoptosis induction, caspase activation and cell cycle arrest at G2M phase
Authors: Wang Hongmei , Wang Yina 
Published: January 2022

Purpose: To investigate the anticancer effects of 7-hydroxycoumarin against cisplatin-resistant ovarian cancer cell line, and the underlying mechanism(s).
Methods: Cell proliferation was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The 4’,6-diamidino-2-phenylindole (DAPI) and acridine orange/ethidium bromide (AO/EB) dual staining methods were used for measuring cell apoptosis in terms of DNA damage. Flow cytometry was used for analysis of mitosis of cancer cells, while protein expression levels were assayed with western blotting.
Results: The 7-hydroxycoumarin preferentially inhibited the proliferation of the ovarian cancer cells, but had significantly less prominent effects on normal cells (p < 0.05). The decrease in cell proliferation was due to induction of cell apoptosis via caspase-linked apoptotic pathway. Treatment with 7- hdoxycoumarin further led to the arrest of cancer cell cycle at G2/M stage (p < 0.05) via down-regulation of the expressions of regulatory proteins that promote mitotic entry.
Conclusion: 7-Hydroxycoumarin exerts significant anticancer effect against cisplatin-resistant ovarian cancer cells via decrease in cell proliferation, induction of apoptosis and mitotic cell cycle arrest. Thus, the compound could emerge as a vital lead molecule in the treatment of cisplatin-resistant type of human ovarian cancer.
MOŽNOSTI ŘEŠENÍ SVOZNĚ ROZVOZNÍ ÚLOHY S HETEROGENNÍM VOZOVÝM PARKEM
Authors: Brázdová Markéta 
Published: January 2022

Příspěvek se zabývá problematikou svozně rozvozních úloh s heterogenním vozovým parkem. Svozně rozvozní úlohy představují složitý optimalizační problém obvykle s mnoha omezujícími podmínkami. Pro úlohu s vozovým parkem zahrnujícím různé typy vozidel charakterizovaných dílčími parametry předkládá článek tři základní varianty řešení úlohy. První možností je seřazení vozidel podle jednoho jejich parametru, druhou možností je rozdělení vozidel do skupin tak, aby všechny parametry vozidel ve skupině byly shodné. Třetí varianta, která je také z hlediska optimalizačního nejvýhodnější, je kombinace třídění vozidel do skupin se současným zohledněním přípustnosti vozidla pro objednávku.
OVLIVŇUJE CENOVÁ KOTVA V DOPRAVĚ VŠECHNY GENERACE STEJNOU MĚROU?
Authors: Pojkarová Kateřina 
Published: January 2022

Mnoho publikací bylo napsáno o působení kognitivních klamů při rozhodování, ať už je tím klamem paradox volby, efekt volavky, haló efekt nebo klam dostupnosti. Tento článek se zaměřuje na efekt kotvy neboli vtisknutí. Lidé mají tendenci nechat se ovlivnit v podstatě jakýmkoli číslem, které uvidí před rozhodováním o ceně zboží a jeho nákupu. Otázkou zůstává, zda se všechny generační skupiny nechají ovlivnit stejným způsobem, nebo jestli jsou různé generace různě citlivé na působení kotvy. Tato otázka byla soustředěna na oblasti rozhodování o ceně letenky, kde proběhl průzkum mezi různými generacemi. Nejen na základě analýzy rozptylu pak bylo možné konstatovat, že mladší generace jsou na vliv kotvy citlivější než ty starší.
INVESTIGASI EKSTRAK BAHAN ALAM SEBAGAI INHIBITOR KOROSI HIJAU PADA BAJA MENGGUNAKAN TEORI FUNGSIONAL KERAPATAN
Authors: Akrom Muhamad 
Published: January 2022

Salah satu material yang ketahanannya terhadap korosi rendah adalah baja ketika berinteraksi dengan lingkungan korosif. Penggunaan green inhibitor mampu memberikan kinerja penghambatan korosi yang baik dengan efisiensi inhibisi yang tinggi pada baja. Green inhibitor yang dalam struktur senyawanya mengandung gugus heteroatom (seperti O, N, S, P) dan cincin aromatik efisien digunakan sebagai inhibitor korosi pada baja. Makalah ini memberikan tinjauan komparatif penting bagi pengembangan green inhibitor ekstrak bahan alam pada baja. Kajian DFT pada level atomik berdasarkan orbital molekuler, parameter kuantum kimia, dan karakteristik adsorpsi menunjukkan hasil yang sesuai dengan hasil eksperimen. Distribusi kerapatan elektron melalui plot Frontier Molecular Orbitals (FMO) menggambarkan prediksi situs aktif melalui distribusi daerah HOMO-LUMO molekul inhibitor yang berinteraksi dengan permukaan baja. Untuk mendapatkan korelasi antara sifat elektronik molekul inhibitor dengan potensi penghambatan (inhibisi) korosi, kalkulasi parameter kimia kuantum seperti potensial ionisasi (I), afinitas elektron (A), kekerasan global (η), elektronegativitas absolut (χ), kelembutan global (σ), fraksi elektron yang ditransfer (ΔN), elektrofilisitas global (ɷ) dan donasi balik elektron (ΔEback-donation) menunjukkan reaktivitas molekul inhibitor yang berpotensi sangat baik untuk dapat berinteraksi dan berikatan kuat dengan permukaan logam, sehingga berpotensi menghasilkan efisiensi inhibisi yang tinggi. Mekanisme inhibisi korosi dapat melalui adsorpsi kimia dan/atau adsorpsi fisika dengan membentuk senyawa kompleks antara molekul inhibitor dengan permukaan baja untuk melindungi dari lingkungan korosif. Pengembangan kajian ke depan harus dapat menampilkan mekanisme interaksi dan inhibisi dari molekul inhibitor secara lebih detail dan sistematis pada level atomik pada beberapa permukaan logam seperti Fe, Al, Cu, dan lainnya.
FREQUENCY OF COMMON FACTORS INFLUENCING THE GRAFT UPTAKE IN MYRINGOPLASTY AMONG THE TERTIARY CARE HOSPITAL KPK
Authors: Khan Azam , Orakzai Khalil Ahmed , Noor Allah , Khan Usman , Khan Sohail , Ullah Mubassir , Ullah Ubaid , Tanveer Tanveer 
Published: January 2022

Introduction: Tympanoplasty refers to any operation involving reconstruction of the tympanic membrane and /or the ossicular chain. Myringoplasty is a tympanoplasty without ossicular reconstruction. Over the years many methods have been used for closing perforations. Myringoplasty was introduced by Berthold in 1878 but the modern era began only in 1950s with the work of Wullstein and Zoellner. The study aims to analyse the common factors which are predictive of success of myringoplasty in adult patients and to construct and validate a prognostic index that could be used as tool to predict the success of myringoplasty in adults.
Objectives: To determine the frequency of common factors influencing the graft uptake in myringoplasty.
Materials and Method: In this study, a total sample size was 376, using 4.08% proportion of fourth degree perineal tear, 95% confidence level and 2% margin of error under WHO software for sample size determination. Moreover, consecutive non probability sampling technique was used.
Results: The mean age was 40 years with standard deviation of ± 2.63. Sixty two percent of the patients were male while thirty eight percent patients were female. The success rate of myringoplasty was 90% while the failure rate was 15(10%) patients in which 4(25%) patients had medium perforation, 5(33%) patients had large perforation while 6(42%) patients had subtotal perforation. Regarding the causes of perforation among 15(10%) patients, 13(85%) patients had infection while only 2 patients had trauma.
Conclusion: The study concludes that infection (85%) was the most common cause of perforation followed by trauma (15%) in the graft uptake in myringoplasty.
An Individualized Algorithm to Predict Mortality in COVID-19 Pneumonia: a Machine Learning Based Study
Authors: Laino Maria Elena , Generali Elena , Tommasini Tobia , Angelotti Giovanni , Aghemo Alessio , Desai Antonio , Morandini Pierandrea , Stefanini Giulio , Lleo Ana , Voza Antonio , Savevski Victor 
Published: January 2022

IntroductionIdentifying SARS-CoV-2 patients at higher risk of mortality is crucial in the management of a pandemic. Artificial intelligence techniques allow to analyze big amount of data to find hidden patterns. We aimed to develop and validate a mortality score at admission for COVID-19 based on high-level machine learning.Material and methodsWe conducted a retrospective cohort study on hospitalized adults COVID-19 patients between March and December 2020. The primary outcome was in-hospital mortality. A machine learning approach on vital parameters, laboratory values, and demographic features was applied to develop different models. Then, a feature importance analysis was performed to reduce the number of variables included in the model, to develop a risk score with good overall performance, that was finally evaluated in terms of discrimination and calibration capabilities. All results underwent cross-validation.Results1,135 consecutive patients (median age 70 years, 64% males) were enrolled, 48 patients were excluded, the cohort was randomly divided in training (760) and test (327). During hospitalization, 251 (22%) patients died. After feature selection, the best performing classifier was random forest (AUC 0.88±0.03). Based on the relative importance of each variable, a pragmatic score was developed, showing good performances (AUC 0.85, ±0.025), and three levels were defined that correlated well with in-hospital mortality.ConclusionsMachine learning techniques were applied in order to develop an accurate in-hospital mortality risk score for COVID-19 based on ten variables. The application of the proposed score has utility in clinical settings to guide the management and prognostication of COVID-19 patients.
Perancangan Sistem Informasi Indeks KAMI
Authors: Burhan Muhammad Burhanur Rizki , Syazili Ahmad 
Published: January 2022

”Universitas Bina Darma memiliki aset teknologi informasi, untuk mengelola aset teknologi informasi Universitas Bina Darma menggunakan standar Indeks KAMI, Universitas Bina Darma pernah mengikuti assessment Indeks KAMI oleh BSSN (Badan Siber dan Sandi Negara) dengan hasil yang belum memuaskan. Karena Universitas Bina Darma belum mempersiapkan dokumen-dokumen Indeks KAMI dengan matang, serta belum adanya sistem untuk mengelola dokumen tersebut. Untuk mengelola dokumen Indeks KAMI Universitas Bina Darma masih menggunakan cara manual dan belum menggunakan sistem, sehingga pengelolaan dokumen Indeks KAMI kurang efektif. Dari uraian diatas Universitas Bina Darma membutuhkan sebuah sistem yang dapat mengelola dokumen yang nantinya memudahkan Universitas Bina Darma mengetahui tingkat kesiapan dan kematangan keamanan informasi.”
Kata Kunci : Website Indeks KAMI.
Upaya Kepala Sekolah dalam Menunjang Profesionalisme Guru di MTs NW 02 Kembang Kerang
Authors: Hidayat Nur Anwar , Mashun Mashun , Munir Muhammad 
Published: January 2022

Penelitian ini bertujuan untuk mengetahui Upaya Kepala Sekolah dalam Menunjang Profesionalisme Guru di MTs NW 02 Kembang Kerang. Jenis penelitian yang digunakan adalah kualitatif dengan menggunakan teknik pengumpulan data berupa observasi, wawancara dan dokumentasi dengan menitik beratkan sumber data informan : Kepala sekolah, Wakil Kepala Sekolah, Staf Tata Usaha dan Guru untuk mengokohkan keabsahan data yang diperoleh.
Hasil penelitian ini mengungkapkan tiga temuan yaitu : (1) Sudah ada Upaya Kepala Sekolah dalam Menunjang Profesionalisme Guru di MTs NW 02 Kembang Kerang dengan mengirimkan guru untuk mengikuti pelatihan, memnerikan surat tugas dan sertifikat pendidikan (2) Sudah ada Langkah-langkah Kepala Sekolah dalam Menunjang Profesionalisme Guru di MTs NW 02 Kembang Kerang dengan membimbing, membina, bekerja sama, dan supervisi dalam Upaya Kepala Sekolah dalam Menunjang Profesionalisme Guru di MTs NW 02 Kembang Kerang (3) sudah ada usaha Kepala Sekolah untuk penyelsaian Kendala-kendala dalam Menunjang Profesionalisme Guru di MTs NW 02 Kembang Kerang.
Efektivitas Penerapan Iklan Digital Pada Era Marketing 4.0 Di Hotel Grand Mirage Resort Bali
Authors: Widahartana I Putu Esa , I Ketut Surata , I Made Rilo Elton 
Published: January 2022

Perkembangan industri mengalami evolusi dari pemasaran konvensional ke pemasaran berbasis digital, atau Marketing 4.0. Penggunaan media sosial sebagai alat promosi penting di zaman sekarang. Di industri hospitaliti, semakin banyak hotel mengembangkan profil mereka di media sosial secara intensif, di antaranya Grand Mirage Resort Bali. Pada awal tahun 2017 hotel telah selesai merenovasi dan rebranding wing baru mereka. Di waktu yang bersamaan, hotel memotong biaya promosi dan fokus untuk menggaet tamu milenial melalui pemasaran digital. Hasilnya, walaupun biaya promosi dipotong dan fokus pada promosi digital, promosi tetap berlangsung dengan baik dan cenderung mengalami peningkatan. Penelitian ini bertujuan untuk mengetahui efektivitas penerapan iklan digital di Grand Mirage Bali serta bagaimana meningkatkan efektivitas penerapan iklan digital di Grand Mirage Bali. Data penelitian dikumpulkan melalui kuesioner dan studi dokumentasi. Data yang sudah terkumpul dianalisis secara deskriptif menggunakan analisis AIDA.

Hasil penelitian menunjukkan (a) iklan digital yang ditampilkan oleh Hotel Grand Mirage Resort Bali sudah efektif dalam penerapannya sebagai media promosi penjualan, dan (b) strategi peningkatan efektivitas penerapan iklan digital di Grand Mirage Bali yaitu memperhatikan pesan yang disampaikan dalam iklan, menggunakan visual yang indah dan atraktif, memilih tema iklan yang sesuai dengan kondisi pasar, serta menggunakan iklan dan trik pemasaran seperti memberikan penawaran terbatas.

Kata Kunci: efektivitas, marketing 4.0, iklan digital, media sosial, website, AIDA
TRACES OF NATIONAL TURKIC CULTURE IN THE KHAKASS' ALYPTYG NYMAKHS
Authors: Маммадли Г. Й. 
Published: January 2022

В данной статье на анализе героических сказаний «Алтын Арыг» и «Книга моего отца Коркута» показано сопоставление с другими сказаниями тюрко - монгольских народов, подчёркнута схожесть типологически сходных явлений. Богатырские сказания хакасского народа отражают в себе историю народа, которая на долгие века сохранила их для будущего поколения всего тюркского мира. Изучение алыптыг нымахов, в частности свадебный обряд (выбор спутника жизни, испытания, девятидневный пиртой, расплетение шестидесяти косичек, заплетение одной косы и т. д.), традиционные сюжеты, действия главных героев показывают их тесную связь с эпосами других тюркских народов. Следы общетюркской культуры встречаются во всех сферах жизни героев героических сказаний. Мотив суженых имеет реальную историко - бытовую основу, восходящую к экзогамии, когда невесту брали из другого рода.
In this article, based on the analysis of "Altyn Aryg" and "The book of my father Korkut', the comparison with other legends of Turkic - Mongolian peoples is shown, and the similarity of typologically similar phenomena is emphasized. The Khakass people's heroic tales reflect the history of the people, which preserved them for the future generation of the entire Turkic world for many centuries to come. The study of alyptyg nymakhs, in particular, the wedding ceremony (choosing a life partner, trials, a nine - day feast - toi, unwinding sixty braids, braiding one braid, etc.), traditional plots and actions of the main characters show their close connection with eposes of other Turkic peoples. Traces of national Turkic culture are found in all spheres of life of heroes of heroic tales. The motif of the betrothed has a real historical and everyday basis, dating back to exogamy, when a bride was taken from another kin.
FOREST IN THE BASHKIRS' VIEW OF THE WORLD
Authors: Абсалямова Ю. А. 
Published: January 2022

В статье анализируются особенности восприятия лесного пространства башкирами. На основе языковых, фольклорных материалов сделана попытка раскрыть различные аспекты взаимоотношений лес - человек, образ леса в картине мира башкир. Как и в большинстве традиционных культур, в целом мифологический образ леса носит отрицательный характер. В фольклоре он часто описывается как тёмный, мрачный, неизвестный, таящий опасности, противопоставляясь обжитому и освоенному пространству селений. Лесной пандемониум также представлен в основном отрицательными персонажами. В целом образ леса в традиционной картине мира башкир предстаёт довольно неоднозначным. С одной стороны - это категория, связанная с потусторонним миром, неизведанная, «чужая» территория. С другой - лес издавна являлся источником различных благ - в виде строительного материала, пушнины, различных продуктов питания, укрывал от врагов.
The article analyzes the features of Bashkirs' perception of the forest space. On the basis of the materials of the epos, folklore, folk ideas, an attempt was made to reveal the various aspects of the relationship between forest and man, the image of the forest in Bashkirs' world view. As in most traditional cultures, the mythological image of the forest as a whole is negative. In folklore, it is often described as dark, gloomy, unknown, fraught with danger, being contrasted with the inhabited and developed space of the villages. The forest pandemonium is also represented mainly by negative characters. On the other hand, in the domestic perception forest is valued for the benefits derived from it: shelter, food, protection from enemies. In addition, Bashkirs, distinguished by a developed aesthetic perception and contemplative thinking, appreciated its beauty, which is also reflected in folklore. In general, the image of forest in the Bashkirs' traditional view of the world appears rather ambiguous. On the one hand, it is the category associated with the other world, unknown, «foreign» territory. On the other hand, the forest has long been a source of various benefits - in the form of construction materials, furs, various food products, and it sheltered them from enemies.