bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti‐MRSA and clot lysis activities of Pestalotiopsis microspora isolated from Dillenia pentagyna Roxb
Authors: Chowdhury Sandip , Ghosh Suvranil , Gond Surendra K. 
Published: August 2022

AbstractThere is an urgent call to search for novel natural compounds against developing multidrug‐resistant microorganisms. The present work focuses on the characterization of a plant‐associated fungus having bioactivity against methicillin‐resistant Staphylococcus aureus (MRSA) strains. A fungal strain P31 was isolated from bark of Dillenia pentagyna and identified as Pestalotiopsis microspora. The maximum anti‐MRSA activity was observed from extract of P31 grown in sabouraud dextrose broth. The minimum inhibitory concentrations (MIC) values of P31 extract were 14 μg/ml for methicillin‐sensitive S. aureus (MSSA) and 32 μg/ml for MRSA strain, respectively. A crude P31 extract showed strong bactericidal activity by killing all treated MRSA cells within 24 h of treatment at their respective MIC value. A scanning electron microscopic study visualized morphological damage of MRSA cells. The membrane permeability of P31 extract‐treated MRSA cells gradually increased which caused release of internal cytoplasmic nucleic acids, proteins and potassium ions (K+) from cells suggesting cell lysis or leakage from cells. A very low concentration of P31 extract was able to inhibit biofilm formed by MRSA cells. Thin layer chromatographic separation followed by gas chromatography‐mass spectrometry analysis of the P31 extract revealed a number of antimicrobial compounds along with an anti‐MRSA compound 2,4‐di‐tert‐butylphenol. In addition, the P31 extract also showed in‐vitro human blood clot lysis activity at various concentrations. The clot lysis activity of P31 extract was found maximum at 500 µg/ml. These findings suggest that fungal isolate P31 has potential as a source of anti‐MRSA compounds useful in staph infections.
The Genetic Landscape of Complex Childhood‐Onset Hyperkinetic Movement Disorders
Authors: Pérez‐Dueñas Belén , Gorman Kathleen , Marcé‐Grau Anna , Ortigoza‐Escobar Juan D. , Macaya Alfons , Danti Federica R. , Barwick Katy , Papandreou Apostolos , Ng Joanne , Meyer Esther , Mohammad Shekeeb S. , Smith Martin , Muntoni Francesco , Munot Pinki , Uusimaa Johanna , Vieira Päivi , Sheridan Eammon , Guerrini Renzo , Cobben Jan , Yilmaz Sanem , De Grandis Elisa , Dale Russell C. , Pons Roser , Peall Kathryn J. , Leuzzi Vincenzo , Kurian Manju A. 
Published: August 2022

ABSTRACTBackground and ObjectiveThe objective of this study was to better delineate the genetic landscape and key clinical characteristics of complex, early‐onset, monogenic hyperkinetic movement disorders.MethodsPatients were recruited from 14 international centers. Participating clinicians completed standardized proformas capturing demographic, clinical, and genetic data. Two pediatric movement disorder experts reviewed available video footage, classifying hyperkinetic movements according to published criteria.ResultsOne hundred forty patients with pathogenic variants in 17 different genes (ADCY5, ATP1A3, DDC, DHPR, FOXG1, GCH1, GNAO1, KMT2B, MICU1, NKX2.1, PDE10A, PTPS, SGCE, SLC2A1, SLC6A3, SPR, and TH) were identified. In the majority, hyperkinetic movements were generalized (77%), with most patients (69%) manifesting combined motor semiologies. Parkinsonism‐dystonia was characteristic of primary neurotransmitter disorders (DDC, DHPR, PTPS, SLC6A3, SPR, TH); chorea predominated in ADCY5‐, ATP1A3‐, FOXG1‐, NKX2.1‐, SLC2A1‐, GNAO1‐, and PDE10A‐related disorders; and stereotypies were a prominent feature in FOXG1‐ and GNAO1‐related disease. Those with generalized hyperkinetic movements had an earlier disease onset than those with focal/segmental distribution (2.5 ± 0.3 vs. 4.7 ± 0.7 years; P = 0.007). Patients with developmental delay also presented with hyperkinetic movements earlier than those with normal neurodevelopment (1.5 ± 2.9 vs. 4.7 ± 3.8 years; P < 0.001). Effective disease‐specific therapies included dopaminergic agents for neurotransmitters disorders, ketogenic diet for glucose transporter deficiency, and deep brain stimulation for SGCE‐, KMT2B‐, and GNAO1‐related hyperkinesia.ConclusionsThis study highlights the complex phenotypes observed in children with genetic hyperkinetic movement disorders that can lead to diagnostic difficulty. We provide a comprehensive analysis of motor semiology to guide physicians in the genetic investigation of these patients, to facilitate early diagnosis, precision medicine treatments, and genetic counseling. © 2022 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society
A multi‐model approach to predict efficacious clinical dose for an anti‐TGF‐β antibody (GC2008) in the treatment of osteogenesis imperfecta
Authors: Mavroudis Panteleimon D. , Pillai Nikhil , Wang Qingping , Pouzin Clemence , Greene Benjamin , Fretland Jennifer 
Published: August 2022

AbstractOsteogenesis imperfecta (OI) is a heterogeneous group of inherited bone dysplasias characterized by reduced skeletal mass and bone fragility. Although the primary manifestation of the disease involves the skeleton, OI is a generalized connective tissue disorder that requires a multidisciplinary treatment approach. Recent studies indicate that application of a transforming growth factor beta (TGF‐β) neutralizing antibody increased bone volume fraction (BVF) and strength in an OI mouse model and improved bone mineral density (BMD) in a small cohort of patients with OI. In this work, we have developed a multitiered quantitative pharmacology approach to predict human efficacious dose of a new anti‐TGF‐β antibody drug candidate (GC2008). This method aims to translate GC2008 pharmacokinetic/pharmacodynamic (PK/PD) relationship in patients, using a number of appropriate mathematical models and available preclinical and clinical data. Compartmental PK linked with an indirect PD effect model was used to characterize both pre‐clinical and clinical PK/PD data and predict a GC2008 dose that would significantly increase BMD or BVF in patients with OI. Furthermore, a physiologically‐based pharmacokinetic model incorporating GC2008 and the body's physiological properties was developed and used to predict a GC2008 dose that would decrease the TGF‐β level in bone to that of healthy individuals. By using multiple models, we aim to reveal information for different aspects of OI disease that will ultimately lead to a more informed dose projection of GC2008 in humans. The different modeling efforts predicted a similar range of pharmacologically relevant doses in patients with OI providing an informed approach for an early clinical dose setting.
Toward the Light‐Operated Superconducting Devices: Circularly Polarized Radiation Manipulates the Current‐Carrying States in Superconducting Rings
Authors: Croitoru Mihail D. , Mironov Sergey V. , Lounis Brahim , Buzdin Alexander I. 
Published: August 2022

AbstractThe ability to rapidly control and manipulate superconducting states is one of the great challenges of modern condensed matter physics. Circularly polarized radiation interacting with a superconducting condensate acts as an effective magnetic field that can generate supercurrents and DC magnetic moments through the inverse Faraday effect (IFE). Using the time‐dependent Ginzburg–Landau (TDGL) equation formalism, the current‐carrying states of a small superconducting ring illuminated by such radiation is calculated. Numerical simulations demonstrate the possibility to on‐demand switch between current‐carrying states in the superconductor by controlling the helicity of the electromagnetic field polarization. This result opens the way to all‐optical operation of superconducting devices.
Improved in situ analysis of lead isotopes in low‐Pb melt inclusions using laser ablation–multi‐collector–inductively coupled plasma–mass spectrometry
Authors: Yu Hongxia , Zhang Yinhui , Liu Xijun , Hong Lubing , Zhang Le , Xu Jifeng , Ren Zhongyuan , Deng Faliang 
Published: August 2022

RationaleIn situ Pb isotope analyses of tiny melt inclusions using laser ablation–multi‐collector–inductively coupled plasma–mass spectrometry (LA–MC–ICP–MS) are crucial for exploring the origins of mafic lavas. However, quantitative use of this technique with low‐Pb (<10 ppm) melt inclusions is difficult due to their low 204Pb content and 204Hg interference.MethodsPb isotopic ratios of various reference glasses and olivine‐hosted melt inclusions were determined using LA–MC–ICP–MS. Multiple ion counters were used to simultaneously determine signal intensities of all Pb isotopes and 202Hg. An Hg signal‐removal smoothing device reduced its signal in the gas blank by >80%. Instrumental mass bias was corrected using the standard–sample bracketing method.ResultsWith 24–90 μm diameter laser spots, 2–4 Hz repetition rates, and 2.5–4 J cm−2 energy fluence, the analytical precisions of 20xPb/204Pb ratios (x = 6, 7, 8) for standards BHVO‐2G, ML3B‐G, NIST 614, NKT‐1G, T1‐G, GOR132‐G, and StHs6/80‐G were <1.0% (2RSD) when 208Pb signals >100 000 cps. The Wangjiadashan melt inclusions have 206Pb/204Pb = 17.14–18.44, 207Pb/204Pb = 15.28–15.66, and 208Pb/204Pb = 37.12–38.68.ConclusionsThe described method improves the precision and accuracy of in situ Pb isotope analysis in low‐Pb melt inclusions using LA–MC–ICP–MS. The Pb isotopic compositions of the Wangjiadashan melt inclusions indicate the coexistence of LoMu and EMII+young HIMU components in the mantle source of weakly alkaline basalts.
Controlling the Mobility of Ionic Liquids in the Nanopores of MOFs by Adjusting the Pore Size: From Conduction Collapse by Mutual Pore Blocking to Unhindered Ion Transport
Authors: Zhang Zejun , Liu Modan , Li Chun , Wenzel Wolfgang , Heinke Lars 
Published: August 2022

AbstractIonic liquids (ILs) in nanoporous confinement are the core of many supercapacitors and batteries, where the mobility of the nanoconfined ILs is crucial. Here, by combining experiments based on impedance spectroscopy with molecular dynamics simulations, the mobility of a prototype IL in the nanopores of an isoreticular metal‐organic framework (MOF)‐series with different pore sizes is explored, where an external electric field is applied. It has been found that the conduction behavior changes tremendously depend on the pore size. For small‐pore apertures, the IL cations and anions cannot pass the pore window simultaneously, causing the ions to mutually block the pores. This results in a strong concentration dependence of the ionic conduction, where the conduction drops by two orders of magnitude when filling the pores. For large‐pore MOFs, the mutual hindrance of the ions in the pores is small, causing only a small concentration dependence. The cutoff between the large‐pore and small‐pore behavior is approximately the size of a cation‐anion‐dimer and increasing the pore diameter by only 0.2 nm changes the conduction behavior fundamentally. This study shows that the pore aperture size has a substantial effect on the mobility of ions in nanoporous confinement and has to be carefully optimized for realizing highly‐mobile nanoconfined ILs.
Interlayer Spacing Regulation of Molybdenum Selenide Promotes Electrocatalytic Hydrogen Evolution in Alkaline Media
Authors: Zhang LiLi , Shen Shijie , Zhang Jitang , Lin Zhiping , Wang Zongpeng , Zhang Qinghua , Zhong Wenwu , Zhu Liu , Wu Guangfeng 
Published: August 2022

AbstractThe construction of heterostructures is a versatile tactic to enhance catalytic activity. However, it is still elusive to realize the modulation of the interlayer spacing in this way to further improve the performance. Here, strong interfacial coupling between CoSe2 and MoSe2 by constructing CoSe2/MoSe2 heterostructures is achieved. The interlayer spacing of MoSe2 is compressed by 0.3 Å. The enhanced charge transfer is validated by X‐ray absorption spectroscopy and X‐ray photoelectron spectroscopy. Coupled with the morphology of hollow microtubes, which can facilitate the exposure of active sites, CoSe2/MoSe2 heterostructures reported here exhibit high activity (119 mV at 10 mA cm−2) and excellent stability with small degradation after 50 h operation, surpassing other analogous powdered electrocatalysts. This work sheds light on the importance of tuning the interlayer spacing to improve electrocatalytic activity.
Metal‐Terpyridine Assembled Functional Materials for Electrochromic, Catalytic and Environmental Applications
Authors: Ranjan Jena Satya , Mandal Tanmoy , Choudhury Joyanta 
Published: August 2022

AbstractMolecular assembly induced by metal‐terpyridine‐based coordinative interactions has become an emergent research topic due to its ease of synthesis and diverse applications. This article highlights recent significant developments in the metal‐terpyridine‐based supramolecular architectures. At first, the design aspect of the molecular building blocks has been described, followed by elaboration on how the ligand backbone plays an important role for achieving different dimensionalities of the resulting assemblies which exhibit a wide range of potential applications. After that, we discussed different synthetic approaches for constructing these assemblies, and finally, we focused on their significant developments in three specific areas, viz., electrochromic materials, catalysis and a new application in wastewater treatment. In the field of electrochromic materials, these assemblies made important advancements in various aspects like sub‐second switching time (<1 s), low switching voltage (<1 V), increased switching stability (>10000 cycles), tuning of multiple colors by using multimetallic systems, fabrication of charge storing electrochromic devices, utilizing and storing solar energy etc. Similarly, the catalysis field witnessed application of the metal‐terpyridine assemblies in C−H monohalogenation, heterogeneous Suzuki‐Miyaura coupling, photocatalysis, reduction of carbon dioxide, etc. Finally, the environmental application of these coordination assemblies includes capturing Cr(VI) from waste water efficiently with high capture capacity, good recyclability, wide pH independency etc.
Di(2‐ethylhexyl) phthalate induced thyroid toxicity via endoplasmic reticulum stress: In vivo and in vitro study
Authors: Xu Qi , Zhao Tianyang , Ri Hyonju , Ye Jiaming , Zhao Weisen , Zhang Yuezhu , Ye Lin 
Published: August 2022

AbstractDi(2‐ethylhexyl) phthalate (DEHP) could induce thyroid injury but the mechanism was unclear. This study combined in vivo and in vitro experiments to clarify the mechanism. In vivo, the offspring of Sprague Dawley rats were gavaged with different doses of DEHP (5, 50, and 250 mg/[kg⋅d]) from in utero to 12 weeks‐old. Transcriptome sequencing was used to detect the mRNA expression profile of the offspring's thyroids. Differentially expressed genes were identified, followed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. In vitro, Nthy‐ori 3–1 cells were exposed to DEHP's metabolite mono (2‐ethylhexyl) phthalate (MEHP) to verify the pathway we found by KEGG analysis. The results indicated that DEHP could disorder the thyroid hormones. Compared with the offspring in control group, the mRNA levels of 656 genes were upregulated in the offspring exposed to 50 mg/(kg⋅d) DEHP. The upregulated genes were enriched in the pathway of “protein processing in the endoplasmic reticulum (ER).” It indicated that the ER stress might play significant role in the thyroid toxicity induced by DEHP. In vitro, the mitochondrial membrane potential (ΔΨm) level of cells was decreased while the reactive oxygen species level was increased after MEHP exposure. MEHP increased the intracellular Ca2+ level and induced ER stress. After ER stress was inhibited by the 4‐phenylbutyric acid, the thyroid toxicity caused by MEHP was alleviated. Taken together, our results indicated that DEHP could induce thyroid toxicity by activating ER stress.
Oesophageal obstruction caused by ingestion of rice chaff used as bedding material in four Japanese black calves
Authors: Takahashi Fumiaki , Kanno Chihiro , Maeda Yosuke , Sato Shogo , Takai Shinji 
Published: August 2022

AbstractFour suckling Japanese black calves developed oesophageal obstruction caused by ingestion of rice chaff used as bedding material. Farmers initially observed milk regurgitation from the nostrils in all four cases, whereas excessive salivation occurred in only one calf. Local veterinarians unsuccessfully attempted to remove the oesophageal obstruction using a gastric tube. Also, the four calves were given liquid nutrition or milk using gastric tube as primary care performed by the referring veterinarians. Due to the significant deterioration of general condition, mucosal hyperaemia, inability to or difficulty standing and marked dehydration, the calves were admitted to the University Hospital. Oesophageal obstruction consisting of rice chaff was confirmed in all cases by endoscopy. Chaff was removed from the oesophagus using an endoscope, and all cases quickly recovered. These cases highlight the danger of using rice chaff as bedding for suckling calves.
Storytelling Practice in Sectors of Education, Psychology, Communication, Marketing: A Narrative Review
Authors: di Furia Marco , Nicoli Nicholas , Akyar Özgür Yasar , Rossi Martina 
Published: August 2022

AbstractChanges in digital storytelling are increasing the platforms for people in different professions such as business, family, healthcare, and research to communicate. Social movements through digital storytelling practices have increased over the past decade due to digital storytelling evolvement. Social activities through social media platforms have impacted freedom of communication, and continuous changes in digital storytelling will create more space for people to raise views. Other practices affected by the evolvement of digital storytelling include marketing, healthcare such as mental health of people, teaching, and research. Generally, digital storytelling changes over the past ten years have improved communication skills, enhanced interpersonal relationships, teamwork, and interprofessional collaboration globally.
On the Perceptions of Online Learning Due to COVID-19 Pandemic. Case Study: University of Foggia, Italy
Authors: del Gobbo Emiliano , Guarino Alfonso , Cafarelli Barbara , Grilli Luca , Limone Pierpaolo 
Published: August 2022

AbstractDue to COVID-19, higher education institutions all over the world transitioned to online learning. The sudden and forced transition to this new learning methodology pushed the Universities to rapidly adequate to the needs, upgrading their digital platforms to comply with the new requirements. In the same way, teachers had to adapt their teaching to fit the new medium’s potentials and limitations. The final receivers of this striking change, the students, had to adequate to the novelty approach, though this process has not been painless. Several difficulties, challenges and opportunities arose in this transition process for students, and the full digital class delivery also stressed them emotively. This study explores University of Foggia students’ perceptions of the emergency online learning. The factors analyzed involved their perception about the University implementation of the online class delivery, their consideration about the future of online learning and their emotional impact in attending courses with this methodology. Quantitative and qualitative data were collected from 3,140 participants. The findings present how students have globally appreciated online learning, but they mostly prefer a blended learning approach. Furthermore, by using segmentation variables, differences emerged among the participants’ groups, indicating online learning can have great potentials, but more complex and integrated approaches are needed to fulfill the different learning needs.
Non-operative vs. operative treatment for multiple rib fractures after blunt thoracic trauma: a multicenter prospective cohort study
Authors: Hoepelman Ruben J. , Beeres Frank. J. P. , Beks Reinier B. , Sweet Arthur A. R. , Ijpma Frank F. , Lansink Koen W. W. , van Wageningen Bas , Tromp Tjarda N. , Link Björn-Christian , van Veelen Nicole M. , Hoogendoorn Jochem. M. , de Jong Mirjam B. , van Baal Mark. C. P. , Leenen Luke P. H. , Groenwold Rolf H. H. , Houwert Roderick M. 
Published: August 2022

Abstract
Background
Patients with multiple rib fractures without a clinical flail chest are increasingly being treated with rib fixation; however, high-quality evidence to support this development is lacking.

Methods
We conducted a prospective multicenter observational study comparing rib fixation to non-operative treatment in all patients aged 18 years and older with computed tomography confirmed multiple rib fractures without a clinical flail chest. Three centers performed rib fixation as standard of care. For adequate comparison, the other three centers performed only non-operative treatment. As such clinical equipoise formed the basis for the comparison in this study. Patients were matched using propensity score matching.

Results
In total 927 patients with multiple rib fractures were included. In the three hospitals that performed rib fixation, 80 (14%) out of 591 patients underwent rib fixation. From the nonoperative centers, on average 71 patients were adequately matched to 71 rib fixation patients after propensity score matching. Rib fixation was associated with an increase in hospital length of stay (HLOS) of 4.9 days (95%CI 0.8–9.1, p = 0.02) and a decrease in quality of life (QoL) measured by the EQ5D questionnaire at 1 year of 0.1 (95% CI − 0.2–0.0, p = 0.035) compared to non-operative treatment. A subgroup analysis of patients who received operative care within 72 h showed a similar decrease in QoL. Up to 22 patients (28%) who underwent surgery experienced implant-related irritation.

Conclusions
We found no benefits and only detrimental effects associated with rib fixation. Based on these results, we do not recommend rib fixation as the standard of care for patients with multiple rib fractures.

Trial registration
Registered in the Netherlands Trial Register NTR6833 on 13/11/2017.