bluuebunny/crossref_metadata_2025_split huggingface dataset:
Women with polycystic ovary syndrome need consultation support from the health care providers: A Qualitative Study
Authors: Kazemi Ashraf , Farajzadegan Ziba , Salehi Mehrdad , ZareMobini Fatemeh 
Published: August 2022

Abstract
Background
Despite the fact that women with polycystic ovary syndrome (PCOS) experience many psychological problems, the need of these women to planning appropriate to improve their mental health seems to be ignored. Therefore, this study will conduct to exploring needs of women's with PCOS using a qualitative approach in Iranian sociocultural context.
Methods
Five women with PCOS and seven health care providers participated in individual semi-structured interviews. Sampling was purposive and continued until data saturation was reached. Data analysis was based on conventional content analysis. The validity of the data was verified through measures including credibility, transferability, dependability, confirmability and authenticity.
Results
Data analysis yielded 3 main categories and 7 sub categories including: (1) Need to reassurance and consultation by health care providers (The positive effect of health care providers counseling on the patient's psychological, Importance of receiving confidence from the health care providers that the disease can be controlled); (2) providing counseling services to improve patient awareness and stress management (Patient stress due to receiving insufficient information about the disease and its complications, the health care provider neglects to provide the patient with comprehensive information on how to manage and treat the disease and (3) the importance of Providing consulting services by a multidisciplinary team (The health care provider neglect of patients' psychological health, proposing the establishment of a specialized place to receive care by a multidisciplinary team, the positive effect of providing services as by a multidisciplinary team to improve the patient's psychological condition).
Conclusion
Controlling the psychological problems of woman with PCOS needs the consultation support of health care providers and comprehensive and long-term planning by a multidisciplinary team to promote the mental health of these women.
A Scalable Cybersecurity Framework for Anomaly Detection in User Behaviour
Authors: Folino Gianluigi , Godano Carla Otranto , Pisani Francesco Sergio 
Published: August 2022

Abstract
Nowadays, the speed of the user and application logs is so quick that it is almost impossible to analyse them in real-time without using scalable systems and platforms. In cybersecurity, human behaviour is responsible directly or indirectly for the most common attacks (i.e., ransomware and phishing). To monitor user behaviour, it is necessary to process fast user logs coming from different and heterogeneous sources, having part of the data or some entire sources missing. A scalable framework based on the Elastic Stack (ELK) to process and store log data from different users and applications is proposed for this aim. This system generates an ensemble of models to classify user behaviour and detect anomalies in real-time. The scalability of the system is guaranteed by the ELK-based software architecture, running on top of a Kubernetes platform. In addition, a distributed evolutionary algorithm is used to classify the users by exploiting their digital footprints derived from many data sources. Experiments conducted on two real-life datasets verify the approach's goodness in detecting anomalies in the user behaviour, coping with missing data and lowering the number of false alarms.
The influence of freezing on the nail withdrawal capacity of Norway Spruce and European Larch wood
Authors: Gašparík Miroslav , Karami Elham , Kytka Tomáš , Das Sumanta , Houska Tomáš 
Published: August 2022

Abstract
Environmental conditions and temperature oscillations can influence wood properties. Accordingly, the performance of wood connections such as nails could be affected by freezing and heating temperature. Thus, in the present work, the impact of freezing/heating temperatures on the nail withdrawal power of Norway Spruce (Picea abies (L.) H. Karst.) and European Larch (Larix decidua Mill.) has been studied. Three types of the wood nail; smooth, helically threaded, and annularly threaded were studied, and two different freezing heating steps have been implemented (-15°C/70°C and − 25°C/70°C). Withdrawal capacity was distinguished on two various anatomical directions; radial and tangential surfaces. The freezing could cause a considerable destructive impact on the nail withdrawal capacity of both wood species. The highest drop has found for smooth nail (A) for larch wood in the tangential direction with 73% and 69% decrease and radial direction, with 70% and 63%, at temperatures − 15°C/70°C and − 25°C/70°C, respectively. Furthermore, spruce wood exhibited a meaningful reduction for both tangential (69% and 63%) and radial (54% and 56%) directions and smooth nail (A), at temperatures − 15°C/70°C and − 25°C/70°C, respectively. The highest reduction was found for annularly threaded nails (C).
Association of a genetic variant in Angiopoietin-like 3 with HDL-C and risk of cardiovascular disease: MASHAD cohort study over 10 years
Authors: Aghasizadeh Malihe , Hoseini Asieh Ahmadi , Sahebi Reza , Kazemi Tooba , Zare-Feyzabadi Reza , Esmaily Habibollah , Samadi Sara , Avan Amir , Ferns Gordon A. , Khosravi Saeede , Ghazizadeh Hamideh , Miri-Moghaddam Ebrahim , Ghayour-Mobarhan Majid 
Published: August 2022

Abstract
Background
Angiopoietin-like 3 (ANGPTL3)-deficiency due to loss-of-function variants are being reported to have a link with triglyceride (TG) level, and high-density lipoprotein cholesterol (HDL-C), and thereby affecting the risk of cardiovascular disease (CVD).
Objective
In the present study, we examined the association of rs10789117 in the ANGPTL 3 gene locus and the risk of CVD in the group of people who were part of the group of Mashhad-Stroke and Heart-Atherosclerotic-Disorders (MASHAD) cohort.
Method
1002 cases with/without CVD following 10 years follow-up were recruited from the MASHAD cohort, followed by DNA extraction and genotyping. The association of the variant with CVD even and lipid profile was assessed using Univariate and Multivariate analysis.
Result
our data showed that CVD patients after 10 years follow up with AC/CC genotypes have been related to higher risk of CVD events compared to AA genotype (OR = 1.43, 95%CI = 1.01–2.02, P = 0.041) as well as its C allele with CVD risk (OR = 1.32, 95%CI = 1.06–1.72, P value = 0.038). Moreover, we realized that significant association between the variant and serum TG and HDL-C.
Conclusion
We demonstrated the potential value of g.14079A > C in ANGPTL3 in CVD, indicating further investigations of the emerging biomarker in a multi-center setting as a risk stratification biomarker in cardiovascular disease.
Learning process analysis of robotic lateral pelvic lymph node dissection for local advanced rectal cancer: CUSUM-curve of 78 consecutive patients
Authors: Lei Xing , Zhang Lei , Qin Qian , Hu Chenhao , Shi Feiyu , Zhang Zhe , She Junjun 
Published: August 2022

Abstract

PurposeRobotic lateral lymph node dissection (LLND) has been suggested as a safe and feasible procedure for local advanced rectal cancer. However, no large case series has documented the learning curve for this technically demanding procedure.The aim of the present study was to evaluate the learning curve for robotic-assisted LLND for local advanced rectal cancer by a surgeon at a single institute.MethodsData on 78 consecutive patients who underwent robotic-LLND operation for rectal cancer were collected to evaluate surgical outcomes, postoperative urinary function changes and mid-term oncological outcomes. The learning curve was analyzed using the cumulative sum (CUSUM) method to assess changes in the unilateral LLND operative times across the case sequence.ResultsAmong the 78 patients, 52 patients underwent bilateral LLND and 28 patients underwent unilateral LLND. A total of 130 consecutive data were recorded. We arranged unilateral robotic-LLND operative times and calculated cumulative sum values, allowing differentiation of three phases: phase I (learning period), Cases 1–51; phase II (proficiency period), Cases 52–83; and phase III (mastery period), Cases 84–130. As the learning curve accumulated, the operation time and estimated blood loss of unilateral robotic-LLND decreased significantly with each phase (P＜0.05). At 12months after surgery, the International Prostatic Symptom Score of patients at phase III was significant lower than phase I (P＜0.05). No significant difference was observed in the overall survival, relapse-free survival and local recurrence among the three phases of the learning curve (P＞0.05).ConclusionThe CUSUM curve shows three phases in the learning and use of robotic-LLND which correspond to the phases of initial learning of the technique, proficiency and mastery. The data obtained suggest that the performance of robotic-LLND is safe in all of the three phases of the learning curve. And, the estimated learning curve for robotic assisted rectal-LLND is achieved after 51 cases.
Ramadan diurnal intermittent fasting is associated with significant plasma metabolomics changes in subjects with overweight and obese: A prospective cohort study
Authors: Faris MoezAlIslam , Madkour Mohamed , Bustanji Yasser Al , Semreen Mohammad , Giddey Alexander D , Soares Nelson C , Zeb Falak 
Published: August 2022

Abstract

During the holy month of Ramadan, adult healthy Muslims are mandated to abstain from dawn to sunset, with free eating night hours that may extend up to 12 hours. The current work was designed to investigate the metabolomics changes incurred upon the observance of Ramadan diurnal intermittent fasting (RDIF). Twenty-five metabolically healthy participants with overweight and obesity (7 females and 18 males, with a mean age of 39.48 ± 10.0 years) were recruited for the study and were followed before and at the end of RDIF month. Dietary, anthropometric, biochemical, and physical activity assessments were performed before and at the end of the fasting month. The metabolomic assay was performed using liquid chromatography-mass spectrometry for the two-time points. Metabolomics assay revealed a significant reduction in a few metabolites. The analysis revealed that 27 metabolites differed significantly (P < 0.05) between pre-and post-RDIF. Among the differentially abundant metabolites, 23 showed a decrease with fasting, these included several amino acids such as aspartame, tryptophan, phenylalanine, histidine, and other metabolites including valeric acid, and cortisol. On the other hand, only four metabolites showed increased levels with RDIF including traumatic acid, 2-pyrrolidinone, PC(18:1(9Z)/18:1(9Z)), and L-sorbose. The MetaboAnalyst® platform reported that the top enriched metabolic pathways included: (1) histidine metabolism; (2) folate biosynthesis (3) phenylalanine, tyrosine, and tryptophan biosynthesis; (3) aminoacyl-tRNA biosynthesis; (3) caffeine metabolism (4) vitamin B6 metabolism; and several other pathways relating to lipid metabolisms such as arachidonic acid metabolism, glycerophospholipid metabolism, and linoleic acid metabolism. In conclusion, RDIF entails significant changes in various metabolic pathways that reflect different dietary and lifestyle behaviors practiced during the fasting month.
Study on modulation bandwidth of GaN-based micro-light-emitting diodes by adjusting quantum well structure
Authors: Yin Pan , Zhi Ting , Tao Tao , Liu Xiaoyan 
Published: August 2022

Abstract

GaN-based blue micro-light-emitting diodes (µ-LEDs) with different structures were designed, of which the effect of quantum well (QW) structure on modulation bandwidth was numerically explored. By using trapezoidal QWs, the quantum-confined Stark effect (QCSE) can be reduced, leading to an enhanced electron-hole wave function overlap, thereby increasing the recombination rate and reducing the differential carrier lifetime. In addition, the improved hole transport also creates favorable conditions for shortening the differential carrier lifetime. It is found that the − 3 dB modulation bandwidth of µ-LEDs with trapezoidal QWs was higher than those of ordinary ones. In this work, the − 3 dB modulation bandwidth of µ-LEDs can be raised to 1.13 GHz at 10 kA/cm2.
Plant species-specific basecaller improves actual accuracy of nanopore sequencing
Authors: Ferguson Scott , McLay Todd , Andrew Rose L. , Bruhl Jeremy J. , Schwessinger Benjamin , Borevitz Justin , Jones Ashley 
Published: August 2022

Abstract
Background
Long-read sequencing platforms offered by Oxford Nanopore Technologies (ONT) allow native DNA containing epigenetic modifications to be directly sequenced, but can be limited by lower per-base accuracies. A key step post-sequencing is basecalling, the process of converting raw electrical signals produced by the sequencing device into nucleotide sequences. This is challenging as current basecallers are primarily based on mixtures of model species for training. Here we utilise both ONT PromethION and higher accuracy PacBio Sequel II HiFi sequencing on two plants, Phebalium stellatum and Xanthorrhoea johnsonii, to train species-specific basecaller models with the aim of improving per-base accuracy. We investigate sequencing accuracies achieved by ONT basecallers and assess accuracy gains by training single-species and species-specific basecaller models. We also evaluate accuracy gains from ONT’s improved flowcells (R10.4, FLO-PRO112) and sequencing kits (SQK-LSK112). For the truth dataset for both model training and accuracy assessment, we developed highly accurate, contiguous diploid reference genomes with PacBio Sequel II HiFi reads.
Results
Basecalling with ONT Guppy 5 and 6 super-accurate gave almost identical results, attaining read accuracies of 91.96% and 94.15%. Guppy’s plant-specific model gave highly mixed results, attaining read accuracies of 91.47% and 96.18%. Species-specific basecalling models improved read accuracy, attaining 93.24% and 95.16% read accuracies. R10.4 sequencing kits also improve sequencing accuracy, attaining read accuracies of 95.46% (super-accurate) and 96.87% (species-specific).
Conclusions
The use of a single mixed-species basecaller model, such as ONT Guppy super-accurate, may be reducing the accuracy of nanopore sequencing, due to conflicting genome biology within the training dataset and study species. Training of single-species and genome-specific basecaller models improves read accuracy. Studies that aim to do large-scale long-read genotyping would primarily benefit from training their own basecalling models. Such studies could use sequencing accuracy gains and improving bioinformatics tools to improve study outcomes.
Integrative multi-omics analysis reveals the landscape of Cyclin-Dependent Kinase (CDK) family genes in pan-cancer
Authors: Wang Peng , Xie Mingjie , Yang Dongmei , Wang Feiran , Chen Erlin 
Published: August 2022

Abstract
Objective
Cyclin-Dependent Kinases (CDKs) get widely involved in cancer development. However, A wealth of conflicting data raise the question of whether CDKs serve as oncogenes or cancer suppressors. Direct evidence from a same-batch cohort with matched multi-omics sequencing data is still lacking.
Methods
Here, we integrated multi-omics analysis to explore CDKs across multiple cancer types using data from The Cancer Genome Atlas (TCGA) database. First, we evaluated the expression levels of CDKs in pan-cancer. Second, we conducted copy number variants (CNV) and somatic mutation analysis of CDKs in pan-caner. Third, the biological functions of CDKs were obtained through pathway analysis. Finally, in order to explore effective drugs for tumors with obvious effects of CDKs, drug sensitivity analysis is also explored.
Results
To our surprise, CDKs are overexpressed in a wide range of cancers (39 upregulated, 12 downregulated cases), among which CDK1 is the top overexpressed gene (7 of 10 cancers). We next observed that CDK gene regions are heterozygously amplificated in cancer genomes. Further pathway analysis revealed that CDK genes cancer hallmarks such as apoptosis and cell cycle. CDK genes associate with 58 drug sensitivity including Trametinib and I-BET-762.
Conclusions
Taken together, our study identified a panel of clinically relevant CDK genes, defined the genomic/epigenomic/transcriptomic landscape of CDKs at a system level, and opened potential therapeutic opportunities for cancer patients.
Intensity, Frequency and Coverage of Hydro-Meteorological Droughts and Agriculture in the Semi-Arid Region of Maharashtra (India)
Authors: Todmal Rahul 
Published: August 2022

Abstract
The present investigation attempted to understand the intensity, frequency and spatial coverage of rainfall, runoff, groundwater and agricultural droughts in the semi-arid region of Maharashtra during 1981–2014. For this, the SPI, SRI, SWI and SCPI indices of probabilistic nature were applied. The linear regression, partial correlation and Student’s t-Test techniques were also used to evaluate inter-connections in hydro-meteorological and agricultural droughts. The hydrological deficiencies mimic the pattern of meteorological droughts in the study area with respect to coverage and intensity. The frequency of moderate hydro-meteorological droughts is observed to be high (once in 3 to 4 years). The study also highlighted that the frequency and intensity of hydrological droughts were increased during the post-1990 period which perhaps associated with anthropogenic interventions. Although the El Niño events resulted in below-average rainfall, runoff and groundwater level in the study area, other phenomena such as EQUINOO/IOD may have played an important role in the occurrence of major droughts in 1986, 2003 and 2012 (occur once in > 30 years). The hydro-meteorological droughts lead to agricultural droughts, as the rainfall and groundwater deficits significantly affected the rainfed as well as irrigated crops (productivity and cropped area). It is notably observed during the severe and region-wide droughts in 1985-86, 2002-03 and 2011-12. Furthermore, this investigation suggested that the study area is very likely to experience hydro-meteorological deficiencies in 2029-30, 2040 and 2050 along with a significant rise in temperature (by 1.05°C) which will exacerbate the water scarcity and agricultural distress in the future (up to 2050).
Manajemen Waka Kesiswaan dalam Mengoptimalkan Kegiatan Pramuka di SMP N 1 Boja
Authors: Faidah Nur , Fatkuroji Fatkuroji , Pamungkas Fanzal 
Published: August 2022

Penelitian ini bertujuan untuk mendeskripsikan manajemen waka kesiswaan dalam mengoptimalkan kegiatan pramuka di SMP N 1 Boja. Penelitian ini menggunakan jenis penelitian lapangan kualitatif. Dengan menggunakan teknik pengumpulan data melalui wawancara, observasi, dan dokumentasi. Kemudian untuk mengecek keabsahan data tersebut dilakukan melalui teknik triangulasi pengumpulan data. Rumusan masalah dalam penelitian ini meliputi: (1) bagaimana perencanaan waka kesiswaan dalam mengoptimalkan kegiatan pramuka di SMP N 1 Boja. (2) bagaimana pelaksanaan waka kesiswaan dalam mengoptimalkan kegiatan pramuka di SMP N 1 Boja. (3) bagaimana evaluasi waka kesiswaan dalam mengoptimalkan kegiatan pramuka di SMP N 1 Boja. Hasil penelitian menunjukkan bahwa: (1) Perencanaan manajemen kesiswaan dalam mengoptimalkan kegiatan pramuka di SMP N 1 Boja adalah mengadakan rapat bersama untuk membahas hal-hal seperti: penyusunan kegiatan, penjadwalan serta pengalokasian waktu kegiatan, dan sistem evaluasi peserta didik. (2) Pelaksanaan manajemen kesiswaan dalam mengoptimalkan kegiatan pramuka di SMP N 1 Boja berjalan baik. Penyusunan kegiatan sudah berjalan sesuai panduan pengembangan diri dan ekstrakurikuler, penjadwalan dan alokasi waktu kegiatan juga sudah sesuai dengan program pramuka, dan sistem evaluasinya menggunakan 3 jenis tes. (3) Evaluasi manajemen kesiswaan dalam mengoptimalkan kegiatan pramuka di SMP N 1 Boja berjalan optimal. Kegiatan pramuka memberi dampak positif sehingga dapat menambah pengetahuan dan keterampilan bagi siswa.
Semiquantitative microbiological risk assessment for water reuse in agriculture: a case study in Brazil
Authors: Damaceno Maria Gabriela de Souza , Cruvinel Karla Alcione da Silva , Santos Ana Silvia Pereira 
Published: August 2022

Abstract
Water reuse is a feasible alternative for non-potable and potable needs, e.g., irrigation. Nonetheless, this option is associated with environmental and public health risks. Therefore, a microbiological health risk assessment was carried out regarding the application of agricultural reuse of water in the neighborhood of Wastewater Treatment Plants (WWTP). This study was carried out in cities in the central region of Brazil, using a semiquantitative methodology. Escherichia coli density in treated wastewater was used as an input, which was obtained from the city wastewater utility. The same exposure scenario was defined for two crops – sugarcane and pasture – at the surroundings of four WWTP under study. As receptors, the following were adopted: farmers; water reuse transport workers; the local community; and sugarcane industry workers. The estimated risks for all groups were considered acceptable. Furthermore, such risks should be reduced to despicable (despicable risk should be understood as low risk) if improvements in the wastewater treatment system and more efficient configurations of barriers are adopted. It can be concluded that risk assessment clarifies the options for system-management, allowing for better informed decision-making and encouraging public confidence in the safe application of water reuse.
Fate, occurrence, and removal of estrogens in livestock wastewaters
Authors: Gomes Fernanda Bento Rosa , Fernandes Pedro Antônio Alves , Bottrel Sue Ellen Costa , Brandt Emanuel Manfred Freire , Pereira Renata de Oliveira 
Published: August 2022

Abstract
During the last decades, livestock and animal feeding operations have been expanded. In parallel, these activities are among the major sources of estrogens in the environment. Thus, considering the environmental and health risks associated with estrogenic compounds, this work reviews the fate, occurrence, and removal of free and conjugated E1, E2, and E3 in livestock wastewaters. A systematic literature review was carried out, and after applying the eligibility criteria, 66 peer-reviewed papers were selected. Results suggest high estrogen concentrations and, consequently, high estrogenic activity, especially in samples from swine farming. E1 and E2 are frequently found in wastewaters from bovine, swine, and other livestock effluents. Aerobic treatment processes were more efficient for estrogen removal, whereas anaerobic systems seem poorly effective. Removal efficiencies of estrogens and estrogenic activity of up to 90% were reported for constructed wetlands, advanced pond systems, trickling filters, membrane bioreactors, aerated and nitrifying reactors, combined air flotation, and vegetable oil capture processes. High concentrations found in wastewaters from livestock allied to the removal efficiencies reported for anaerobic processes (usually used to treat livestock wastewaters) evidence the importance of monitoring these compounds in environmental matrices.