bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical characteristics and gene mutation analysis of a family with hereditary spastic paraplegia type 11
Authors: Luo Aidi , Xu Zucai , Liao Shusheng 
Published: November 2022

Abstract
Background：Autosomal recessive spastic paraplegia with thinning of corpus callosum (ARHSP-TCC) is a complex hereditary spastic paraplegia. Spastic Paraplegia gene 11(SPG11) is the most common ARHSP-TCC. The study of SPG11 in China is small in scale, and only a few gene mutations have been reported .
Case Presentation:We reported a family with ARHSP-TCC. The proband presented spastic gait and cognitive impairment. Brain MRI showed thinning of corpus callosum. The proband's brother shows only a spastic gait. A detailed history, physical examination, and supplementary examination were performed to rule out the acquired cause of spastic paraplegia. In order to identify pathogenic mutations, we used target sequence capture sequencing technology to detect HSP-related genes in family members, combined with Sanger sequencing verification, and found two new complex heterozygous mutations of SPG11: c.6738_6739insT and c.5934_5935insTAACCTGGAA. The codon of Glu2247 amino acid changed to stop codon (p.Glu2247Ter) and that of Val1979 amino acid changed to stop codon (p.Val1979ter), respectively. Bioinformatics analysis predicted that these mutations would result in a loss of protein function.
Conclusions: We found two novel complex heterozygous mutations of SPG11 : c.6738_6739insT and c.5934_5935insTAACCTGGAA，which enriches the phenotype of SPG11 mutations.
Low Concentration PAMAM Dendrimer Coated Superparamagnetic Iron Oxide Nanoparticles – Potentials in MR Imaging and Theranostics
Authors: Lotey Navjeet Kaur , Chaughule Ramesh , Pednekar Suhas 
Published: November 2022

Abstract
MRI is a highly sensitive, non-invasive diagnostic tools which reveals information from within deep layers of tissues. Contrast agents are used to enhance the image contrast to detect otherwise difficult to identify morphological anomalies. There is need and scope to improvise the contrast properties and biocompatibility quotient of the existing agents. In this research we’ve explored dendrimer coated SPIONs as highly effective contrast agents with excellent in-vitro biocompatibility. They exhibit significant contrast in T2 weighted images in very low concentrations which is studied using a clinical MR imager. It was concluded that although SPIONs alone exhibit the highest contrast efficacy, the coating with dendrimers improvises their biocompatibility and dispersion even at very low concentrations.
Can 2-hour Indirect Calorimetry Measurement Accurately Predict 24-hour Energy Expenditure in Critically Ill Surgical Patients?
Authors: Lichter Yael , Sold Oded , Angel Yoel , Nizri Eran , Gerstenhaber Fabian , Oz Amir Gal , Stavi Dekel , Nini Asaph , Singer Pierre , Goder Noam 
Published: November 2022

Abstract
Background
Measuring energy expenditure (EE) by indirect calorimetry (IC) has become the gold standard tool for critically ill patients in order to define energy targets and tailor nutrition. Debate remains as to the optimal duration of measurements, or the optimal time of day in which to perform IC.
Methods
In this retrospective observational study, we analyzed results of daily continuous IC in 270 mechanically ventilated, critically ill patients, admitted to the surgical intensive care unit (SICU) in a tertiary medical center, and compared measurements performed at different hours of the day.
Results
A total of 51,448 IC hours was recorded, with an average 24-hour EE of 1523 ± 443 kcal/day. Night shift (00:00–8:00) was found to have a significantly lower EE measurements (mean 1498 kcal/day, 95% CI 1445–1551), than afternoon (16:00–00:00, mean 1526 kcal/day 95% CI 1473–1577) and morning (8:00–16:00, mean 1539 kcal/day, 95% CI 1483–1594) measurements (p < 0.001 for all). The bihourly time frame which most closely resembled the daily mean was 18:00–19:59, with a mean of 1523 ± 434 kcal/day. Daily EE measurements of the continuous IC at days 3–7 of admission showed a trend towards a daily increase in 24-hour EE, but the difference was not statistically significant (p = 0.077).
Conclusions
Periodic measurements of EE can differ slightly when performed in various hours of the day, but the error range is small and may not necessarily have a clinical impact. When continuous IC is not available, a 2-hour EE measurement between 18:00–19:59 can serve as a reasonable alternative.
A study on integrated management of life-responsive data to secure social safety net for the vulnerable class
Authors: won anna , Lee Ji-Ae , Hong wonhwa 
Published: November 2022

Abstract
The purpose of this study is to secure a social safety net such as the prevention of loneliness by targeting the elderly and single households for the vulnerable class in this study. In this regard, elements that are essential in basic life were defined as life-responsive information, and methods for data collection and linkage with the welfare system were sought. Life responsive data was defined as indoor motion detection and door open detection sensors based on the energy consumption data required by home appliances used on a daily basis. In addition, public data such as electric power companies were linked in order to link with the energy welfare system. As a result, various life-response information was collected according to the characteristics of each household, and the characteristics of an elderly single household were derived. In the future, in order to secure energy welfare and social safety net at the same time, it is thought that it will be possible to collect data accumulated over the four seasons and use it to secure a safety net for the elderly.
Assessment of common carp testis as an alternative to 17α Methyltestosterone for sex reversal of Nile Tilapia (Oreochromisniloticus)
Authors: Asad Farkhanda , Tasadaq Manahil , Jamal Rafia , Anwar Noshaba 
Published: November 2022

Abstract
The sex reversion technique in fish is widespread in many countries. The present study aimed to produce a monosex populations of all male Nile tilapia (Oreochromis spp.) using 17α-methyltestosterone (17α-MT) and common carp testes (as a source of natural androgen). The experiment was divided into two consecutive phases, the first was fry administration with negative control (without hormone) and positive control (with hormone)feed viz.,MT1:60mg/kg, MT2:70mg/kg (17α-MT),carp testis CT1:70% and CT2:80%for 30 days to reverse the sex of male fish and second phase was nursing the fingerlings for two months on control diet (32%CP).Two hundred fry of 4–5 days old were randomly stocked in five aquariums each with one replicate (20fry/aquarium)using completely randomized design (CRD). Results revealed a significant growth rate (P < 0.05) in control group. In case of proximal chemical composition of body meat, CT2 treatment showed maximum deposition of crude protein, crude fat, and ash whereas dry matter showed maximum in MT2 and CT1 treatments. The highest male proportion (95% ± 0.58%) was recorded at 70mg/kg MT, which was significantly higher (P < 0.05) than other groups.These results suggested that synthetic and natural androgen supplementation in the diet play a significant role in improving the growth performance and body composition. Furthermore, synthetic androgen compared to natural androgens played a significant role to induce sex inversion.
Factors Associated with Delayed Diagnosis of Crohn's Disease: A Systematic Review and Meta-Analysis
Authors: Chen Miao feng , Shao Rong , Wang Wenrui , Xie Jinping 
Published: November 2022

Abstract
BACKGROUND
Delayed diagnosis is a major barrier to the effective management of Crohn's disease (CD). Several studies have investigated factors responsible for delays in diagnosis, but no meta-analyses have systematically assessed the impact of these factors.
AIM
To assess the impact of various factors on the delayed diagnosis of CD.
METHODS
PubMed, EMBASE, and Web of Science databases were searched to identify observational studies published before April 2022 that assessed factors associated with delays in CD diagnosis. We excluded review articles, case reports, or commentaries without original data.We pooled effect sizes as odds ratios (OR) using random effects models for each risk factor examined in at least three distinct samples. We assessed study quality on the basis of the Newcastle-Ottawa Scale and examined between-study heterogeneity. A funnel plot was used for visual assessment of publication bias. The study protocol was registered with PROSPERO, CRD42022322251.
RESULTS
A total of 18 studies were included in the paper, covering 13 countries. The study sample consisted of 9,669 cases. Ileal CD (OR =1.46, 95% CI =1.21–1.76), smoking at the time of diagnosis (OR =1.19, 95% CI =1.02–1.38), and use of NSAIDs (OR =1.34, 95% CI =1.04–1.72) were significantly associated with a delay in CD diagnosis.
CONCLUSION
The findings suggest that ileal CD, use of NSAIDs, and smoking are risk factors for the delayed diagnosis of CD. Education of patients and primary care providers about these risk factors should be increased.
Application of silicon coupled with sulfate improve the drought tolerance in sunflower via modulating the morpho-physiological and antioxidants defense mechanisms
Authors: Nisar Muhammad , Aqeel Muhammad , Sattar Abdul , Sher Ahmad , Ijaz Muhammad , Ul-Allah Sami , Irfan Muhammad , Javaid Muhammad Mansoor , Rasheed Umair , Ali Muhammad 
Published: November 2022

AbstractBackground ​Sunflower (Helianthus annuusL.) is one of the main oilseed crops as an essential dietary component used for human and animal feed. Due to changing climate scenarios, severe drought conditions adversely affect growth and productivity of sunflower. The current study was planned to investigate the potential of sole and combined application of silicon (Si) and sulfate (SO4) to improve sunflowers' morpho-physiological, yield and yield traits under water deficit conditions. Methods There were two factors in the experimental treatments; i) two levels of irrigation regimes, 80% water holding capacity (WHC) and 40% WHC and ii) control (Ck), water spray, 4.0 mM Si, 4.0 mM SO4and Si + SO4(4.0 mM + 4.0 mM). Drought stress was imposed at the 50% heading stage of sunflower. Results Drought stress reduced leaf chlorophyll pigments and relative water contents. In contrast, it diminished the growth and production of sunflower by reducing plant height, stem girth, head diameter, leaf area index, numbers of achene per head, 100-achene weight, biological yield and achene yield per plant. Results of the study indicated that Chl a, b, and a + b and relative water contents significantly increased with sole and combined application of Si and SO4under drought stress condition. Moreover; remarkably improved antioxidant activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) was recorded along with the accumulation of soluble proline and protein with the combined application of Si and SO4under drought stress. The combined application of Si and SO4improved the sunflower growth by enhancing plant height (17%), stem girth (17.66%), head diameter (18.15%), leaf area index (17.80%), number of achene per head (19.46%), 100- achene weight (10.70%), biological yield (17.12%) and achene yield per plant (17.21%). Conclusions The combined application of Si and SO4is the best option to alleviate the adversities of drought stress for the growth and development of sunflower.
Lower Attenuation and Higher Kurtosis of Coronary Artery Calcification Associated with Vulnerable Plaque – An Agatston Score Propensity-matched CT Radiomics study
Authors: Huang Eric , Lin Huey-Shyan , Chen Yi-Chun , Li Yi-He , Huang Yi-Luan , Ju Yu-Jeng , Yu Hsien-Chung , Kicska Gregory A. , Wu Ming-Ting 
Published: November 2022

Abstract
Background:
Coronary artery calcification (CAC) burden assessed by Agatston score (AS) is currently recommended to stratify patients at risk for future acute coronary syndrome (ACS). Besides the CAC burden, the biostructure of CAC may also play a vital role in the vulnerability of CAC, which CT radiomics could reveal. Propensity-score matching of the traditional risk factors and CAC burden between the ACS and asymptomatic groups could radically remove biases and allow exploration of characteristic features of CAC in ACS.
Methods:
We retrospectively identified 77 patients with ACS who had a CAC scan before percutaneous coronary intervention between 2016–2019. These 77 patients were one-to-two propensity-score matched for traditional risk factors of ACS and AS ranks to select 154 subjects from 2890 asymptomatic subjects. A validation cohort of 30 subjects was also enrolled. Radiomics features of each plaque were extracted and averaged in each person. Conditional logistic regression and area under curve analysis was used for statistical analysis.
Results:
A higher number of coronary segments involved, lower mean, median, first quartile, and standard deviation of attenuation, and increased kurtosis of attenuation of CAC were associated with the ACS group compared to the control group (p < 0.05 for all). Multivariable analysis showed that the lower median attenuation (OR = 0.969, p < 0.001) and higher Kurtosis (OR = 18.7, p < 0.001) were associated with the ACS group. The median attenuation and kurtosis significantly increase across AS ranks 1 to 4 (p = 0.001). The AUC of kurtosis (0.727) and median attenuation (0.66) were both significantly higher than that of the standard AS (AUC = 0.502) and the number of TRF (AUC = 0.537). The best cut-off of kurtosis at 2.74 yielded an accuracy of 74%, and the cut-off of median attenuation at 196 yielded an accuracy of 68%. The accuracy of kurtosis was 64%, and the accuracy of median attenuation was 55% in the validation cohort.
Conclusion:
After propensity-matching traditional risk factors and CAC burden, CT radiomics highlighted that lower median attenuation and higher kurtosis were the CAC characteristics of vulnerable plaques. These features improve the understanding of the biomechanics of CAC evolution and enhance the value of CAC scan in ACS risk assessment.
Debris and mud-flows runout assessment: a comparison among empirical geometric equations in the Giampilieri and Briga basins (east Sicily, Italy) affected by the event of 1st October 2009
Authors: Falconi Luca Maria , Moretti Lorenzo , Puglisi Claudio , Righini Gaia 
Published: November 2022

Abstract

Empirical/geometric methods rely on simple geometrical connections between some landslide parameters and the runout distance reached by the displaced material. Despite the extreme simplification of the dynamic of this landslide typology, those methods can provide useful information about the propagation of this shallow and fast landslide typology, joining reliability of the results with easiness of use.
The objective of this work is to compare the efficacy of different geometric relationships for the identification of the runout distances in a debris and mud-flows prone test area located in Sicily, southern Italy, where several events were analysed, and a consistent set of data was collected and processed.
Notwithstanding some uncertainties in the methodological approach and not negligible scattering between expected and observed runout distances, the use of such geometric approaches, together with the evaluation of kinematic parameters such as velocity and kinetic energy, can significantly boost the implementation of site-specific analyses for a more detailed definition of landslides susceptibility at local scale.
Targeted Vibration-Induced Necrosis in Cancer Cells using Microrobots
Authors: Mallick Sudipta , Sokolich Max , Rivas David , Das Sambeeta 
Published: November 2022

Abstract
Therapeutic delivery of anti-cancer drugs is a major goal of modern medicine. In particular, microrobots (MRs) have recently been studied for their ability to navigate difficult-to-reach regions in the human body to deliver therapeutics for microscopically localized interventions. However, the control of individual and swarms of MRs to precisely target localized cellular regions remains a significant challenge, preventing their applications as delivery systems in cancer research. In this study, magnetic MRs were used to target cancer cells and create localized magnetic oscillations which resulted in magnetolysis of cancer cells. The magnetic MRs were selectively steered towards Hepatocarcinoma cells (HepG2 cells) using our custom-built magnetic controller under a rotating magnetic field at different frequencies. After internalization of the microrobots by cancer cells, magnetic oscillation of varying dosages was applied to disrupt the internal structure of cancer cells which leads to subsequent cell death.
Puerperal sepsis: Bacterial profile, Antimicrobial susceptibility patterns and Associated factors at Asella Referral and Teaching Hospital, Central Ethiopia: Cross sectional study
Authors: Godana Abduselam Abbiso , Gashaw Mulatu , Abdulsemed Kedir Abdella , Adere Fikru , Gebrie Getenet Beyene 
Published: November 2022

Abstract
Background: Sepsis is a major cause of maternal death worldwide and caused by a variety of bacterial pathogens. In many developing countries including Ethiopia, the treatment of puerperal sepsis is based on empirical/syndromic that may promote antimicrobial resistance. Therefore the aim of this study was to investigate bacterial pathogens, their antimicrobial susceptibility patterns, and associated factors among women with suspected puerperal sepsis.
Methods: A cross-sectional study was conducted among puerperal sepsis suspected women attending Asella Referral and Teaching Hospital, from September 2020 to August 2021. A total of 174 study participants were enrolled. Sociodemographic and obstetric data of the participants were collected using a pretested structured questionnaire and checklist respectively. About 20 ml blood sample was collected from all study participants into BacT/ALERT® 3D blood culture bottles and incubated into BacT/ALERT® 3D automated blood culture system. Endocervical swab was also collected into Aime's transport media. Bacterial isolation and identification was done using standard bacteriological methods. Disc diffusion method was used to determine the antimicrobial susceptibility profiles of bacterial isolates. Data were entered into EpiData version 4.6 and transferred to SPSS version 25.0 for analysis.
Results: The overall positivity rate of bacterial isolates among puerperal sepsis suspected women was 48.9%. Out of these 87.1% of the isolates were Gram negative bacteria. The most common isolates were E. coli (54.1%) followed by Klebsiella spp. (23.5%) and S. aureus (10.6%). E. coli showed a higher resistance rate to Piperacillin (87%). Klebsiella spp. showed a higher resistance rate to Aztreonam (65%) and Ceftriaxone (65%). S. aureus showed a higher resistance rate to Trimethoprim-sulfamethoxazole (66.6%). In this study, 81.2% of the isolates were multi-drug resistant bacterial pathogens. Multivariate regression analysis showed no statistically significant association between sociodemographic, obstetrics factors, and having bacteria.
Conclusion: In this study the overall positivity rate in this study was 48.9%. E. coli, Klebsiella species, and S. aureus were the most common isolated bacteria. High numbers of multidrug-resistant bacterial isolates were identified. Our finding emphasizes the need for strengthening microbiology services for better management of patients.
The effects of restricted access to healthcare and education on vulnerable people: an analysis of the determinants of health and education outcomes during the COVID-19 pandemic
Authors: Gineikytė-Kanclerė Vaida , Klimavičiūtė Luka , Schito Marco 
Published: November 2022

Abstract
This study investigates how restrictions in healthcare access and school closures in European countries during the COVID-19 pandemic affected health and education outcomes among two groups of vulnerable people: the elderly and the youth. These groups are vulnerable in that they are more susceptible to social changes and less capable of adapting, either because of individual characteristics or pre-existing structural inequalities. We employ multi-level modelling using data from SHARE and Eurofound’s survey on living and working under COVID-19. The findings show that elderly people have, on average, perceived a worsening in general and mental health, especially among women and those to whom medical care was restricted. Students suffered the most when their households’ socio-economic status and digital divides presented significant barriers to adapting to online schooling. Our findings corroborate national-level studies from both within and outside the EU, particularly concerning the gender dimension, access to healthcare, pre-existing socio-economic inequalities, and digital divides. We conclude with some policy implications and the necessity to craft preventive policies to make the social system more resilient in responding to future crises.
Experimental and numerical investigations of cutting forces and chip formation during micro-cutting of Ti42Nb titanium alloy produced by laser-based powder bed fusion
Authors: BOUBAKER Houssemeddine BEN , Le-Coz Gael , Moufki abdelhadi , Nouari Mohammed , Laheurte Pascal 
Published: November 2022

Abstract
Ti-Nb alloys are high-profile candidates for the biomedical applications. However, because of poor surface integrity (i.e. residual stress and surface roughness), Ti-Nb implantable medical devices need to be machined in order to obtain functional surfaces finish. In this work, experimentaland numerical investigations are conducted to study the micro-cutting response of Ti42Nb titanium alloy produced by laser-based powder bed fusion. Experimental micro-cutting tests are carried out using precision turning lathe. Trials are performed with two cutting velocities of 60 m/min and 120 m/min and different feed rates, varying from 5 to 40μm/rev. For the numerical study, a porous crystal plasticity-based model is proposed to address the impact of anisotropy and microstructure heterogeneities of the polycrystalline material. The crystal plasticity-basedmodel is identified using strain–stress curves obtained from compression tests performed under two strain rates and a wide range of temperatures. Numerical micro-cutting simulations are performed in order to gain insight into the impact of microstructural features (i.e. crystallographic orientation and grain size) on the machinability of the alloy.According to the results, the effect of the strain rates and the temperature on the thermomechanical behavior of the Ti42Nb titanium alloy produced by laser-based powder bed fusion is correctly depicted. The model captured the strain localization on adiabatic shear band during compression tests. According to the micro-cutting simulations, the local variables such as temperature, damage and plastic deformation are strongly impacted by the crystallographic orientations and the grain size.In addition, depending on the crystallographic orientations, the chip morphology changes form continues, slightly segmented to largely segmented.