bluuebunny/crossref_metadata_2025_split huggingface dataset:
A cross-scale analysis to understand and quantify effects of photosynthetic enhancement on crop growth and yield
Authors: Wu Alex , Brider Jason , Busch Florian A. , Chen Min , Chenu Karine , Clarke Victoria C. , Collins Brian , Ermakova Maria , Evans John R. , Farquhar Graham D. , Forster Britta , Furbank Robert T. , Gorszmann Michael , Hernandez Miguel A. , Long Benedict M. , Mclean Greg , Potgieter Andries , Price G. Dean , Sharwood Robert E. , Stower Michael , van Oosterom Erik , von Caemmerer Susanne , Whitney Spencer M. , Hammer Graeme L. 
Published: July 2022

AbstractPhotosynthetic manipulation provides new opportunities for enhancing crop yield. However, understanding and quantifying effectively how the seasonal growth and yield dynamics of target crops might be affected over a wide range of environments is limited. Using a state-of-the-art cross-scale model we predicted crop-level impacts of a broad list of promising photosynthesis manipulation strategies for C3 wheat and C4 sorghum. The manipulation targets have varying effects on the enzyme-limited (Ac) and electron transport-limited (Aj) rates of photosynthesis. In the top decile of seasonal outcomes, yield gains with the list of manipulations were predicted to be modest, ranging between 0 and 8%, depending on the crop type and manipulation. To achieve the higher yield gains, large increases in both Ac and Aj are needed. This could likely be achieved by stacking Rubisco function and electron transport chain enhancements or installing a full CO2 concentrating system. However, photosynthetic enhancement influences the timing and severity of water and nitrogen stress on the crop, confounding yield outcomes. Strategies enhancing Ac alone offers more consistent but smaller yield gains across environments, Aj enhancement alone offers higher gains but is undesirable in less favourable environments. Understanding and quantifying complex cross-scale interactions between photosynthesis and crop yield will challenge and stimulate photosynthesis and crop research.Summary StatementLeaf–canopy–crop prediction using a state-of-the-art cross-scale model improves understanding of how photosynthetic manipulation alters wheat and sorghum growth and yield dynamics. This generates novel insights for quantifying impacts of photosynthetic enhancement on crop yield across environments.
Contractile ring composition dictates kinetics ofin silicocontractility
Authors: Cortes Daniel B. , Nedelec Francois , Maddox Amy Shaub 
Published: July 2022

AbstractConstriction kinetics of the cytokinetic ring are expected to depend on dynamic adjustment of ring composition, but the impact of component abundance dynamics on ring constriction is understudied. Computational models generally assume that contractile networks maintain constant composition. To test how compositional dynamics affect constriction kinetics, we first measured F-actin, non-muscle myosin II, septin, and anillin duringC. eleganszygotic mitosis. A custom microfluidic device that positioned the cell with the division plane parallel to a light sheet allowed even illumination of the cytokinetic ring. Measured component abundances were implemented in an agent-based 3D model of a membrane-associated contractile ring. With constant network composition, constriction occurred with biologically unrealistic kinetics. Measured changes in component quantities elicited realistic constriction kinetics. Simulated networks were more sensitive to changes in motor and filament amounts, than that of crosslinkers and tethers. Our findings highlight the importance of network composition for actomyosin contraction kinetics.SummaryWe created a microfluidic device compatible with high numerical aperture light sheet microscopy to measure cytokinetic ring component abundance in the C. elegans zygote. Implementing measured dynamics into our three-dimensional agent-based model of a contractile ring elicited biologically realistic kinetics.
Evaluation of ERIC-PCR and MALDI-TOF as typing tools for multidrug resistant Klebsiella pneumoniae clinical isolates from a tertiary care center in India
Authors: Kundu Jyoti , Kansal Shubhangi , Rathore Shivali , Kaundal Meenakshi , Angrup Archana , Biswal Manisha , Walia Kamini , Ray Pallab 
Published: July 2022

AbstractBackground and AimMultidrug resistant Klebsiella pneumoniae is associated with nosocomial infections in both outbreak and non-outbreak situations. The study intends to evaluate the potential of enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), a genomic based typing and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) proteomic-based typing techniques for clonal relatedness among multidrug resistant Klebsiella pneumoniae isolates.MethodologyMultidrug resistant clinical isolates of Klebsiella pneumoniae (n =137) were collected from March 2019 to February 2020. Identification and protein-based phylogenetic analysis were performed by MALDI-TOF MS. Genomic typing was done by ERIC-PCR and analyzed by an online data analysis service (PyElph). Dice method with unweighted pair group method with arithmetic mean (UPGMA) program was used to compare the ERIC profiles. The samples were also evaluated by PCR for the presence of genes encoding carbapenemases, extended spectrum beta lactamases (ESBLs) and mobile colistin resistance-1 (mcr1).Result and ConclusionIsolates were typed into 40 ERIC types, and six groups by MALDI-TOF-MS. PCR-based analysis revealed that all the strains harbored two or more ESBL and carbapenemase genes. None of the isolates revealed the presence of the plasmid mediated mcr-1 gene for colistin resistance. The study presents ERIC based typing as more robust in comparison to MALDI-TOF for finding the clonal relatedness in epidemiological studies.
A phylogenomically informed five-order system for the closest relatives of land plants
Authors: Hess Sebastian , Williams Shelby K. , Busch Anna , Irisarri Iker , Delwiche Charles F. , de Vries Sophie , Darienko Tatyana , Roger Andrew J. , Archibald John M. , Buschmann Henrik , von Schwartzenberg Klaus , de Vries Jan 
Published: July 2022

SUMMARYThe evolution of streptophytes had a profound impact on life on Earth. They brought forth those photosynthetic eukaryotes that today dominate the macroscopic flora: the land plants (Embryophyta) [1]. There is convincing evidence that the unicellular/filamentous Zygnematophyceae—and not the morphologically more elaborate Coleochaetophyceae or Charophyceae—are the closest algal relatives of land plants [2, 3, 4, 5, 6]. Despite the species richness (>4,000), wide distribution, and key evolutionary position of the zygnematophytes, their internal phylogeny remains largely unresolved [7, 8]. There are also putative zygnematophytes with interesting body plan modifications (e.g., filamentous growth) whose phylogenetic affiliations remain unknown. Here, we studied a filamentous green alga (strain MZCH580) from an Austrian peat bog with central or parietal chloroplasts that lack discernible pyrenoids. It representsMougeotiopsis calosporaPALLA, an enigmatic alga that was described more than 120 years ago [9], but never subjected to molecular analyses. We generated transcriptomic data ofM. calosporastrain MZCH580, and conducted comprehensive phylogenomic analyses (326 nuclear loci) for 46 taxonomically diverse zygnematophytes. Strain MZCH580 falls in a deep-branching zygnematophycean clade together with some unicellular species, and thus represents a formerly unknown zygnematophycean lineage with filamentous growth. Our well-supported phylogenomic tree lets us propose a new five-order system for the Zygnematophyceae, and provides evidence for at least five independent origins of true filamentous growth in the closest algal relatives of land plants. This phylogeny provides a robust and comprehensive framework for performing comparative analyses and inferring the evolution of cellular traits and body plans in the closest relatives of land plants.
Insect homolog of oxytocin/vasopressin associated with parenting of males but not females in a subsocial beetle
Authors: Potticary Ahva L. , Cunningham Christopher B. , McKinney Elizabeth C. , Moore Patricia J. , Belay Amsale T. , Moore Allen J. 
Published: July 2022

AbstractParental care is thought to evolve through modification of behavioral precursors, which predicts that the mechanistic changes occur in the genes underlying those traits. The duplicated gene system of oxytocin/vasopressin has been broadly co-opted across vertebrates to influence parenting, from a pre-duplication ancestral role in water balance. It remains unclear whether co-option of these genes for parenting is limited to vertebrates. Here, we experimentally tested for associations betweeninotocingene expression and water balance, parental acceptance of offspring, and active parenting in the subsocial beetleNicrophorus orbicollis, to test whether a single copy homologue,inotocin, has similarly been co-opted for parental care in a species with elaborate parenting. As expected,inotocinwas associated with water balance in both sexes.Inotocinexpression increased around sexual maturation in both males and females, although more clearly in males. Finally, we found that expression ofinotocinwas not associated with acceptance of larvae but was associated with a transition to male but not female parenting. Moreover, level of offspring provisioning behavior and gene expression were positively correlated in males but uncorrelated in females. Our results suggest a broad co-option of this system for parenting that may have existed prior to gene duplication, and that inotocin may be associated with flexibility in parenting behavior.ImpactSummaryOxytocin/vasopressin are amongst the most studied neuropeptides in vertebrates, influencing water balance, mating interactions, and most notably, social bonding. This gene pair evolved from a duplication in the vertebrate lineage of an ancestral vasopressin-like gene. Are the multiple social effects in vertebrates due to this duplication, or are social influences also ancestral? Here, we demonstrate that, in a biparental social beetle with a single copy, inotocin is associated with social interactions between fathers and offspring as well as being associated with the ancestral role of water balance in both males and females. In vertebrates, both oxytocin and vasopressin have been shown to impact social interactions in both sexes, although often showing sex-specificity in their action within species. Our results suggest that this system may have been co-opted for parenting prior to gene duplication and may facilitate flexibility in caring behavior.
IDENTIFICATION AND QUANTIFICATION OF MATRISOME PROTEINS OF MOUSE KIDNEYS
Authors: Rende Umut , Ahn Seong Beom , Adhikari Subash , Guller Anna 
Published: July 2022

ABSTRACTExtracellular matrix (ECM) is essential for tissue homeostasis. Understanding the matrisome (ECM proteome) composition and mechanisms of ECM control in health and disease is crucial for discovering therapeutic agents and diagnostic tools for inflammatory, fibrotic and cancerous conditions. The challenging obstacle in the ECM analysis is the need to optimise matrisome enrichment methods for different organs, diseases, and species. Currently, there is no optimized protocol nor a publicly available matrisome database for mouse kidneys. This limits the power of murine models in renal diseases and development research. In this study, we comparatively explored the matrisome of healthy C57BL/6 mice using two matrisome extraction methods, including the Millipore Compartment Fractionation (Method-1) and the Sequential Extraction (Method-2) approaches. We examined the efficiency of these methods in matrisome profiling by LC-MS/MS, protein identification and label-free quantification using MaxQuant. As a result of the study, 113 matrisome proteins were identified, including 22 proteins that have not been previously listed in the Matrisome Database (MD). Method-2 allowed identification and quantification of all core and ECM-associated matrisome proteins detected by Method-1 and additionally revealed more core matrisome and ECM-associated proteins. By characterisation of the murine renal matrisome enhanced by our methodological insights, this study provides critically important information for biological and medical kidney research.
Wastewater-Based Epidemiology for SARS-CoV-2 Biomarkers: Evaluation of Normalization Methods in Small and Large Communities in Southern Germany
Authors: Mitranescu Alexander , Uchaikina Anna , Kau Anna-Sonia , Stange Claudia , Ho Johannes , Tiehm Andreas , Wurzbacher Christian , E. Drewes Jörg 
Published: July 2022

AbstractIn the context of the COVID-19 pandemic, wastewater-based epidemiology (WBE) emerged as a useful tool to account for the prevalence of SARS-CoV-2 infections on a population scale. In this study we analyzed wastewater samples from three large (> 300,000 people served) and four small (< 25,000 people served) communities throughout southern Germany from August to December 2021, capturing the fourth infection wave in Germany dominated by the Delta variant (B.1.617.2). As dilution can skew the SARS-CoV-2 biomarker concentrations in wastewater, normalization to wastewater parameters can improve the relationship between SARS-CoV-2 biomarker data and clinical prevalence data. In this study, we investigated the suitability and performance of various normalization parameters. Influent flow data showed strong relationships to precipitation data; accordingly, flow-normalization reacted distinctly to precipitation events. Normalization by surrogate viruses CrAssphage and Pepper Mild Mottle Virus showed varying performance for different sampling sites. The best normalization performance was achieved with a mixed fecal indicator calculated from both surrogate viruses. Analyzing the temporal and spatial variation of normalization parameters proved to be useful to explain normalization performance. Overall, our findings indicate that the performance of surrogate viruses, flow and hydro-chemical data is site-specific. We recommend to test the suitability of normalization parameters individually for specific sewage systems.TOC Art
Continuing professional education and audit quality: evidence from an emerging market
Authors: Ocak Murat , Ozkan Serdar , Can Gökberk 
Published: July 2022

PurposeIn this paper, the authors examine the association between the amount of continuing professional education (CPE) hours per staff and audit quality in terms of discretionary accruals and audit opinion.Design/methodology/approachSeveral methodologies are adopted to test the hypotheses, including the ordinary least square (OLS) and logistic regression (Logistic). The authors also employ instrument variables regression with two least square (IVREG with 2SLS) and instrument variables probit model (IVProbit) to address the possible endogeneity and strengthen the validity of the main estimation results.FindingsThe main results show that there is a positive and significant relationship between CPE hours per staff and audit quality. As the authors grouped CPE into four areas (finance, auditing and accounting, tax, law and regulations and others) the results are more robust for the sub-sample “accounting and audit” and “others”. Moreover, the findings of this study suggest that CPE hours per staff do not affect audit quality significantly for Big4 audit firms compared to non-Big4 firms.Research limitations/implicationsThe sample size of the present study is quite small because the transparency reports of the audit firms in Turkey have been available since 2013 and the authors could not reach some auditor demographics at the individual level and some attributes at the audit firm level. Besides, some alternative audit quality measures, such as audit effort, audit fees are not employed because they are not disclosed.Originality/valueThis study contributes to the audit literature using Turkish audit firms. The authors believe that the setting of Turkey may yield interesting results because of the data it provides.
Effect of commitment on the link between organizational culture and international project performance: a comparison between China and Korea
Authors: Lin Yi-Hsin , Han Wenqing , Kim Chan Joong , Jiang Li , Xia Nini 
Published: July 2022

PurposeThe purpose of this paper is to verify the mediating role of commitment between market-oriented organizational culture and international market performance, and to discuss the moderator effect of national institutional environment on this mediating role.Design/methodology/approachThe research design follows a mixed methodology, combining qualitative and quantitative data collection and analysis. In the first phase, research hypotheses are proposed based on a literature analysis. In the second phase, sample data are collected through interviews and questionnaires sent to domestic contractors in China and South Korea, and a validity analysis of the results is carried out. Correlation and regression analyses are then performed on the valid data to verify hypotheses to prove the existence and influence of mediating effects. Hayes PROCESS Macro is used on the regression results to test the mediating effect of commitment on international project performance and the moderation effect of institutional environment.FindingsThe results reveal that the commitment between partners has a mediating effect on the relationship between market culture and international project performance; however, no hierarchy culture is revealed. The mediating effect of commitment is regulated by the institutional environment.Research limitations/implicationsAlthough the reliability and validity of the questionnaire data in this study are in line with research standards, a larger sample size would improve the reliability of the results. Further, the interviewed samples are mainly from China and South Korea; large representative samples from additional countries, such as Japan, should be considered to gain a fuller understanding and more comprehensive results.Originality/valueBy emphasizing the differences between the two institutional environments of developing and developed countries in East Asia, a theoretical and empirical basis is provided. International construction enterprises in other countries can apply the findings to improve their international market performance in different institutional environments. The findings also provide an empirical reference that international construction enterprises in China and South Korea may use to adjust their organizational cultures and commitments to improve market performance.
Organizational spirituality: a literature review and research agenda
Authors: Vasconcelos Anselmo Ferreira 
Published: July 2022

Purpose
The purpose of this paper is to ascertain whether the organizational spirituality (OS) also called for spiritually-based organization (SBO) concept continues to be, in fact, a source of refreshing ideas to the organizational studies.

Design/methodology/approach
Regarding that OS is a theme that does not pertain to the scientific mainstream, the criteria to select valuable work were broader. On this basis, this literature review focused on a set of distinct sources: empirical and theoretical-conceptual papers (i.e. peer-reviewed), books, book chapters and doctoral dissertations. The material should be written in English and matched specifically the terms “spiritually-based organization,” “organizational spirituality” and “spiritual organization” in the websites of prominent scientific publications such as Google Scholar, EBSCO, Emerald, Sage and Elsevier. The premise was to portray an ample overview about what the scientific research has been yielded on this topic covering the period of 23 years (i.e. 1999–2022).

Findings
Data revealed that a large portion of the scholarly work of OS has been largely published in journals of modest factor impact or in books/book chapters. Such evidence indicates that OS definitively is not embraced by the scientific mainstream, despite the relevance of this topic. Further, it appears that the study, research and writings about SBOs, despite their positive effects and features, do not draw the attention of the majority of academic community. As a result, this field of knowledge has been characterized by scant work. This conclusion sounds somewhat astonishing considering that companies strongly driven by financial and economic concerns have proved not to be useful to humankind well-being and the planet.

Originality/value
This review sought to concentrate only on scholarly work that could bring something noteworthy to the debate of the OS concept. By the same token, other related concepts such as workplace spirituality and spirituality in the workplace, which have been properly explored, were not approached here. The author surmises that such path may account for somewhat the reduced number of work found about this topic, despite the fact this review drew on Google Scholar as well.
Hope, goal-commitment and -stress mediating between collaborative leadership, financial resources and performance
Authors: Chak Choiwai Maggie , Carminati Lara , Wilderom Celeste P.M. 
Published: July 2022

PurposeCombining the goal-setting and job demands-resources (JD-R) theories, we examine how two project resources, collaborative project leadership and financial project resources, enhance high project performance in community-academic health partnerships.Design/methodology/approachWith a sequential explanatory mixed-method research design, data were collected through a survey (N = 318) and semi-structured interviews (N = 21). A hypothesised three-path mediation model was tested using structural equation modelling with bootstrapping. Qualitative data were examined using thematic analysis.FindingsProject workers’ hope, goal-commitment and -stress: (1) fully mediate the hypothesised relationship between highly collaborative project leadership and high project performance; and (2) partially mediate the relationship between financial project resources and high project performance. The qualitative data corroborate and deepen these findings, revealing the crucial role of hope as a cognitive-motivational facilitator in project workers’ ability to cope with challenges.Practical implicationsProject leaders should promote project workers’ goal commitment, reduce their goal stress and boost project performance by securing financial project resources or reinforcing workers’ hope, e.g. by fostering collaborative project leadership.Originality/valueThe findings contribute to the project management and JD-R literature by considering the joint effects of project workers’ hope and two commonly studied project resources (collaborative project leadership and financial project resources) on high project performance. Moreover, we demonstrate the importance of the goal-setting and JD-R theories for understanding complex health-promotion projects connecting academic to community work.