bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromosome-Level Genomes Reveal the Genetic Basis of Descending Dysploidy and Sex Determination in Morus Plants
Authors: Xia Zhongqiang , Dai Xuelei , Fan Wei , Liu Changying , Zhang Meirong , Bian Peipei , Zhou Yuping , Li Liang , Zhu Baozhong , Liu Shuman , Li Zhengang , Wang Xiling , Yu Maode , Xiang Zhonghuai , Jiang Yu , Zhao Aichun 
Published: August 2022

Abstract
Multiple plant lineages have independently evolved sex chromosomes and variable karyotypes to maintain their sessile lifestyles through constant biological innovation. Morus notabilis, a dioecious mulberry species, has the fewest chromosomes among Morus spp., but the genetic basis of sex determination and karyotype evolution in this species has not been identified. In this study, three high-quality genome assemblies were generated for Morus spp. [including dioecious M. notabilis (male and female) and Morus yunnanensis (female)] with genome sizes of 301–329 Mb and were grouped into six pseudochromosomes. Using a combination of genomic approaches, we found that the putative ancestral karyotype of Morus species was close to 14 protochromosomes, and that several chromosome fusion events resulted in descending dysploidy (2n = 2x = 12). We also characterized a ∼ 6.2-Mb sex-determining region on chromosome 3. Four potential male-specific genes, a partially duplicated DNA helicase gene (named MSDH) and three Ty3_Gypsy long terminal repeat retrotransposons (named MSTG1/2/3), were identified in the Y-linked area and considered to be strong candidate genes for sex determination or differentiation. Population genomic analysis showed that Guangdong accessions in China were genetically similar to Japanese accessions of mulberry. In addition, genomic areas containing selective sweeps that distinguish domesticated mulberry from wild populations in terms of flowering and disease resistance were identified. Our study provides an important genetic resource for sex identification research and molecular breeding in mulberry.
Perturbations of graphs for Newton maps I: bounded hyperbolic components
Authors: GAO YAN , NIE HONGMING 
Published: August 2022

AbstractWe consider graphs consisting of finitely many internal rays for degenerating Newton maps and state a convergence result. As an application, we prove that a hyperbolic component in the moduli space of quartic Newton maps is bounded if and only if every element has degree
$2$
on the immediate basin of each root. This provides the first complete description of bounded hyperbolic components in a complex two-dimensional moduli space.
The effects of international judges’ personal characteristics on their judging
Authors: Maučec Gregor , Dothan Shai 
Published: August 2022

AbstractThis Symposium Issue looks at how personal traits of international judges matter in their judging. The articles selected shed light on the ways that international judges’ personality, that is, their character differences and personal backgrounds, shape, control, or modify their conduct and their rulings. The articles in the Symposium reveal that individual international judges have different personal attributes and identities that affect: (i) different aspects of judicial reasoning, problem-solving and case management; (ii) the ways judges interact, deliberate and affect each other in collectively deciding cases; and (iii) the outcomes of international trials. This Symposium, therefore, looks beyond the traditional ‘legal formalistic’ understanding (prevalent also among international judges themselves) that judges – as impartial, rational, and mechanical decision-makers – simply apply existing, recognized rules or principles of law to the facts and questions before a court in the context of concrete cases without regard to any personal leanings and biases. It examines how and to what extent judges at international courts make their choices conditioned on their personal identity characteristics, and when such characteristics exhibit greater or lesser effects on their decision-making.
Landscape response to deformation in the Sabalan area, NW Iran: Inferred from quantitative morphological and structural analysis
Authors: SABER Reza , CAGLAYAN Ayse , ISIK Veysel 
Published: August 2022

ABSTRACTThe geological and tectonic background of the Sabalan area in NW Iran and its present-day surface processes make it ideal for examining the effects of tectonic processes in shaping the Earth's crust. As a result of the intense distribution of pre-Quaternary and Quaternary structures (e.g., faults, joints and folds), most of the drainage basins in the southern and central parts of the study area have developed under tectonic-dominated conditions, whereas the effects of erosional processes are greater in the north and east. An evaluation of the geomorphic indices using the index of active tectonics (IAT) and analytical hierarchy process (AHP) methods shows that the AHP results are more reliable than the IAT results and are coherent with the geological and structural conditions of the study area. The geomorphic results are highly consistent with the intensity and distribution of fractures. The majority of fractures have developed in a NW–SE direction, indicating antithetic R′ Riedel fractures to the main NE-SW-trending faults. However, a significant number of the fractures in the study area are NNE–SSW- and NE–SW-oriented R and P fractures and NNW–SSE-oriented tension fractures. Palaeostress analysis of the fault data shows at least two faulting events in the pre-Quaternary and Quaternary, respectively. The pre-Quaternary NNW–SSE-striking dextral strike-slip faults experienced post-Eocene 25–30° clockwise rotation and re-activated as NE–SW-striking sinistral faults during the Quaternary. Although seismic activity is currently low, the consistency of our results with the regional stress data show that the study area is still tectonically active.
Impact of celebrity disclosure on mental health-related stigma
Authors: Gronholm Petra C. , Thornicroft Graham 
Published: August 2022

Abstract
Mental health stigma and discrimination are global problems, and their reduction is recognised as an important public health priority. Involving celebrities in stigma reduction is increasingly common. This Editorial considers the impact of celebrity disclosure on mental health-related stigma; that is, whether and how a famous person openly speaking about their experience of mental health conditions can reduce stigma. Potential explanations for how celebrity mental health disclosures can influence mental health-related knowledge, attitudes and behaviours are outlined, followed by an overview of evidence on how celebrity disclosure operates to reduce stigma. Considering the available evidence, we provide a number of conclusions and recommendations for how celebrities can effectively be involved in anti-stigma efforts, and what considerations this requires. It is fair to say that celebrity disclosures can support stigma-reduction efforts through increasing the public's awareness of mental health, modelling behaviour and generating openness on speaking about mental health problems, and on seeking help when needed. However, whether celebrity disclosure achieves changes in mental health stigma-related knowledge, attitudes and behaviours depends on the extent to which there is a match between the attributes of the famous person, the content shared in their disclosure narrative and the intended audience of the message. Further research is needed on all these questions to better understand how to successfully utilise the potentially huge power of celebrity disclosure in large-scale anti-stigma efforts.
Elastic Anisotropy of Lizardite at Subduction Zone Conditions
Authors: Deng Xin , Luo Chenxing , Wentzcovitch Renata M. , Abers Geoffrey A. , Wu Zhongqing 
Published: August 2022

AbstractSubduction zones transport water into Earth's deep interior through slab subduction. Serpentine minerals, the primary hydration product of ultramafic peridotite, are abundant in most subduction zones. Characterization of their high‐temperature elasticity, particularly their anisotropy, will help us better estimate the extent of mantle serpentinization and the Earth's deep water cycle. Lizardite, the low‐temperature polymorph of serpentine, is stable under the P‐T conditions of cold subduction slabs (<260°C at 2 GPa), and its high‐temperature elasticity remains unknown. Here we report ab initio elasticity and acoustic wave velocities of lizardite at P‐T conditions of subduction zones. Our static results agree with previous studies. Its high‐temperature velocities are much higher than previous experimental‐based lizardite estimates with chrysotile but closer to antigorite velocities. The elastic anisotropy of lizardite is much larger than that of antigorite and could better account for the observed large shear‐wave splitting in some cold slabs such as Tonga.
Differential microRNA expression analyses across two brain regions in Alzheimer’s disease
Authors: Dobricic Valerija , Schilling Marcel , Schulz Jessica , Zhu Ling-Shuang , Zhou Chao-Wen , Fuß Janina , Franzenburg Sören , Zhu Ling-Qiang , Parkkinen Laura , Lill Christina M. , Bertram Lars 
Published: August 2022

AbstractDysregulation of microRNAs (miRNAs) is involved in the pathogenesis of neurodegenerative diseases, including Alzheimer’s disease (AD). Hitherto, sample sizes from differential miRNA expression studies in AD are exceedingly small aggravating any biological inference. To overcome this limitation, we investigated six candidate miRNAs in a large collection of brain samples. Brain tissue was derived from superior temporal gyrus (STG) and entorhinal cortex (EC) from 99 AD patients and 91 controls. MiRNA expression was examined by qPCR (STG) or small RNA sequencing (EC). Brain region-dependent differential miRNA expression was investigated in a transgenic AD mouse model using qPCR and FISH. Total RNA sequencing was used to assess differential expression of miRNA target genes. MiR-129-5p, miR-132-5p, and miR-138-5p were significantly downregulated in AD vs. controls both in STG and EC, while miR-125b-5p and miR-501-3p showed no evidence for differential expression in this dataset. In addition, miR-195-5p was significantly upregulated in EC but not STG in AD patients. The brain region-specific pattern of miR-195-5p expression was corroborated in vivo in transgenic AD mice. Total RNA sequencing identified several novel and functionally interesting target genes of these miRNAs involved in synaptic transmission (GABRB1), the immune-system response (HCFC2) or AD-associated differential methylation (SLC16A3). Using two different methods (qPCR and small RNA-seq) in two separate brain regions in 190 individuals we more than doubled the available sample size for most miRNAs tested. Differential gene expression analyses confirm the likely involvement of miR-129-5p, miR-132-5p, miR-138-5p, and miR-195-5p in AD pathogenesis and highlight several novel potentially relevant target mRNAs.
Enhanced ocean oxygenation during Cenozoic warm periods
Authors: Auderset Alexandra , Moretti Simone , Taphorn Björn , Ebner Pia-Rebecca , Kast Emma , Wang Xingchen T. , Schiebel Ralf , Sigman Daniel M. , Haug Gerald H. , Martínez-García Alfredo 
Published: August 2022

AbstractDissolved oxygen (O2) is essential for most ocean ecosystems, fuelling organisms’ respiration and facilitating the cycling of carbon and nutrients. Oxygen measurements have been interpreted to indicate that the ocean’s oxygen-deficient zones (ODZs) are expanding under global warming1,2. However, models provide an unclear picture of future ODZ change in both the near term and the long term3–6. The paleoclimate record can help explore the possible range of ODZ changes in warmer-than-modern periods. Here we use foraminifera-bound nitrogen (N) isotopes to show that water-column denitrification in the eastern tropical North Pacific was greatly reduced during the Middle Miocene Climatic Optimum (MMCO) and the Early Eocene Climatic Optimum (EECO). Because denitrification is restricted to oxygen-poor waters, our results indicate that, in these two Cenozoic periods of sustained warmth, ODZs were contracted, not expanded. ODZ contraction may have arisen from a decrease in upwelling-fuelled biological productivity in the tropical Pacific, which would have reduced oxygen demand in the subsurface. Alternatively, invigoration of deep-water ventilation by the Southern Ocean may have weakened the ocean’s ‘biological carbon pump’, which would have increased deep-ocean oxygen. The mechanism at play would have determined whether the ODZ contractions occurred in step with the warming or took centuries or millennia to develop. Thus, although our results from the Cenozoic do not necessarily apply to the near-term future, they might imply that global warming may eventually cause ODZ contraction.
Non-viral, specifically targeted CAR-T cells achieve high safety and efficacy in B-NHL
Authors: Zhang Jiqin , Hu Yongxian , Yang Jiaxuan , Li Wei , Zhang Mingming , Wang Qingcan , Zhang Linjie , Wei Guoqing , Tian Yue , Zhao Kui , Chen Ang , Tan Binghe , Cui Jiazhen , Li Deqi , Li Yi , Qi Yalei , Wang Dongrui , Wu Yuxuan , Li Dali , Du Bing , Liu Mingyao , Huang He 
Published: August 2022

AbstractRecently, chimeric antigen receptor (CAR)-T cell therapy has shown great promise in treating haematological malignancies1–7. However, CAR-T cell therapy currently has several limitations8–12. Here we successfully developed a two-in-one approach to generate non-viral, gene-specific targeted CAR-T cells through CRISPR–Cas9. Using the optimized protocol, we demonstrated feasibility in a preclinical study by inserting an anti-CD19 CAR cassette into the AAVS1 safe-harbour locus. Furthermore, an innovative type of anti-CD19 CAR-T cell with PD1 integration was developed and showed superior ability to eradicate tumour cells in xenograft models. In adoptive therapy for relapsed/refractory aggressive B cell non-Hodgkin lymphoma (ClinicalTrials.gov, NCT04213469), we observed a high rate (87.5%) of complete remission and durable responses without serious adverse events in eight patients. Notably, these enhanced CAR-T cells were effective even at a low infusion dose and with a low percentage of CAR+ cells. Single-cell analysis showed that the electroporation method resulted in a high percentage of memory T cells in infusion products, and PD1 interference enhanced anti-tumour immune functions, further validating the advantages of non-viral, PD1-integrated CAR-T cells. Collectively, our results demonstrate the high safety and efficacy of non-viral, gene-specific integrated CAR-T cells, thus providing an innovative technology for CAR-T cell therapy.
Promoting pedestrian ecomobility in Riyadh City for sustainable urban development
Authors: Katar Ihab 
Published: August 2022

AbstractAs the world is giving climate change a higher priority, several Coalitions are working on meeting a clean and green environment (SDG 13), focusing on new streets’ design in total urban development. A previous study discussed the pedestrian mobility status in Riyadh city, with attention to sustainable mobility, considering the pedestrians’ traveling patterns that use their cars rather than public transportation. This paper investigates the Ecomobility that seeks to promote the integration of passenging, cycling, wheeling, and walking. This approach will provide better health (SDG 3), clean air, mobility and accessibility, noise avoidance, greenhouse gas emission reduction, individual cost savings, and energy efficiency (SDG 11). It analyzes the pedestrians’ traveling modes in Riyadh city through a case study of the Prince Sultan University’s (PSU) community, then evaluates the pedestrians’ environment quality in the campus surroundings. Analysis used a web-based survey referred to the PSU people, including Students, Faculty, and Staff. On the other hand, the pedestrian’s environment quality was evaluated on-site built on well-constructed criteria. The assessment’s results addressed the factors influencing pedestrian choices and underlined the barriers to transformation to Ecomobility. They revealed that around 73% of the respondents expressed willingness to transform to ecomobility modes. About 52% of the survey sample preferred the public bus option. For the non-motorized options, students preferred cycling and wheeling modes, while faculty members and staff preferred walking. Based on conclusions, recommendations are proposed to foster pedestrian ecomobility for sustainable urban development in Riyadh city.
The importance of visual control and biomechanics in the regulation of gesture-speech synchrony for an individual deprived of proprioceptive feedback of body position.
Authors: Pouw Wim , Harrison Steven J. , Dixon James A. 
Published: August 2022

AbstractDo communicative actions such as gestures fundamentally differ in their control mechanisms from other actions? Evidence for such fundamental differences comes from a classic gesture-speech coordination experiment performed with a person (IW) with deafferentation (McNeill, 2005). Although IW has lost both his primary source of information about body position (i.e., proprioception) and discriminative touch from the neck down, his gesture-speech coordination has been reported to be largely unaffected, even if his vision is blocked. This is surprising because, without vision, his object-directed actions almost completely break down. We examine the hypothesis that IW’s gesture-speech coordination is supported by the biomechanical effects of gesturing on head posture and speech. We find that when vision is blocked, there are micro-scale increases in gesture-speech timing variability, consistent with IW’s reported experience that gesturing is difficult without vision. Supporting the hypothesis that IW exploits biomechanical consequences of the act of gesturing, we find that: (1) gestures with larger physical impulses co-occur with greater head movement, (2) gesture-speech synchrony relates to larger gesture-concurrent head movements (i.e. for bimanual gestures), (3) when vision is blocked, gestures generate more physical impulse, and (4) moments of acoustic prominence couple more with peaks of physical impulse when vision is blocked. It can be concluded that IW’s gesturing ability is not based on a specialized language-based feedforward control as originally concluded from previous research, but is still dependent on a varied means of recurrent feedback from the body.
Genetic pattern and demographic history of cutlassfish (Trichiurus nanhaiensis) in South China Sea by the influence of Pleistocene climatic oscillations
Authors: Gu Sui , Yan Yun-Rong , Yi Mu-Rong , Luo Zhi-Sen , Wen Hui , Jiang Chang-Ping , Lin Hung-Du , He Xiong-Bo 
Published: August 2022

AbstractTrichiurus nanhaiensis is one of the most important commercial fish species in the South China Sea. This study aimed to investigate the level of genetic variation and population genetic structure of T. nanhaiensis in the South China Sea for the first time, using 281 individuals collected from seven locations along the coast of mainland China, Taiwan, and Hainan Island. A high level of haplotype diversity and low nucleotide diversity were detected in the mitochondrial DNA cyt b gene and nuDNA RYR 3 gene. The overall expected heterozygosity (He = 0.693) among the seven populations ranged from 0.681 to 0.706 in microsatellite DNA data, which revealed high levels of genetic diversity. Significant genetic differentiation was found in Taidong populations in Taiwan, revealing the prevention of gene flow caused by the Kuroshio Current. Two major lineages based on the cyt b gene suggested that the Taiwan Strait acted as a geographic barrier for T. nanhaiensis during the glacier periods in the late Pleistocene. The Bayesian skyline plot also revealed that population demographic expansion of T. nanhaiensis was estimated to have occurred in 0.1 Mya. Our results indicated that all populations of T. nanhaiensis had experienced a recent genetic bottleneck following recent expansion based on ABC analysis.
Hand explorations are determined by the characteristics of the perceptual space of real-world materials from silk to sand
Authors: Dövencioǧlu Dicle N. , Üstün F. Seyhun , Doerschner Katja , Drewing Knut 
Published: August 2022

AbstractPerceiving mechanical properties of objects, i.e., how they react to physical forces, is a crucial ability in many aspects of life, from choosing an avocado to picking your clothes. There is, a wide variety of materials that differ substantially in their mechanical properties. For example, both, silk and sand deform and change shape in response to exploration forces, but each does so in very different ways. Studies show that the haptic perceptual space has multiple dimensions corresponding to the physical properties of textures, however in these experiments the range of materials or exploratory movements were restricted. Here we investigate the perceptual dimensionality in a large set of real materials in a free haptic exploration task. Thirty-two participants actively explored deformable and non-deformable materials with their hands and rated them on several attributes. Using the semantic differential technique, video analysis and linear classification, we found four haptic dimensions, each associated with a distinct set of hand and finger movements during active exploration. Taken together our findings suggest that the physical, particularly the mechanical, properties of a material systematically affect how it is explored on a much more fine-grained level than originally thought.
Lipase-mediated Baeyer–Villiger oxidation of benzylcyclopentanones in ester solvents and deep eutectic solvents
Authors: Mazur Marcelina , Janeczko Tomasz , Gładkowski Witold 
Published: August 2022

AbstractThis work presents the chemo-enzymatic Baeyer–Villiger oxidation of α-benzylcyclopentanones in ester solvents as well as deep eutectic solvents (DES). In the first part of the work the effect of selected reaction conditions on the reaction rate was determined. The oxidation process was most effective in ethyl acetate at 55 °C, with the use of lipase B from Candida antarctica immobilized on acrylic resin and UHP as oxidant. Ultimately, these preliminary studies prompted the development of an effective method for the implementation of lipase-mediated Baeyer–Villiger oxidation of benzylcyclopentanones in DES. The highest conversion was indicated when the oxidizing agent was a component of DESs (minimal DESs). The fastest conversion of ketones to lactones was observed in a mixture of choline chloride with urea hydrogen peroxide. In this case, after 3 days, the conversion of the ketones to lactones products exceeded 92% for all substrates. As a result, two new lactones were obtained and fully characterized by spectroscopic data.
Enhanced catalytic activity of MOF-74 via providing additional open metal sites for cyanosilylation of aldehydes
Authors: Jun Hyeji , Oh Sojin , Lee Gihyun , Oh Moonhyun 
Published: August 2022

AbstractThe preparation of metal-organic frameworks (MOFs) having many open metal sites is an excellent approach for the development of highly active MOF-based catalysts. Herein, well-defined rice-shaped MOF-74 microparticles having structural defects are prepared by incorporating two analogous organic linkers [2,5-dihydroxy-1,4-bezenedicarboxylic acid (DHBDC) and 2-hydroxy-1,4-benzenedicarboxylic acid (HBDC)] within the MOF-74 structure. The replacement of some of DHBDC in MOF-74 by HBDC causes the structural defects (excluding some of the bridged hydroxyl groups), and these structural defects provide the additional open metal sites within MOF-74. Finally, the additional open metal sites within MOF-74 result in the enhanced catalytic activity for the cyanosilylation of several aldehydes. A series of MOF-74s is prepared with various incorporated amounts of HBDC, and the optimum ratio between DHBDC and HBDC in MOF-74 to achieving the best catalytic performance is determined. In addition, the defected MOF-74 displays an excellent recyclability for the catalytic reaction.