bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growth of exclusively breastfed small for gestational age term infants in the first six months of life: a prospective cohort study
Authors: Nurani Neti , Wibowo Tunjung , Susilowati Rina , Hastuti Janatin , Julia Madarina , Van Weissenbruch Mirjam M. 
Published: February 2022

AbstractBackgroundCompared to their appropriate-for-gestational-age (AGA) peers, small-for-gestational-age (SGA) infants are prone to growth deficits. As the first 6 months of exclusive breastfeeding is generally recommended, it is essential to understand how this intervention might impact SGA infants’ growth. This study aims to assess growth of exclusively breastfed SGA term infants in the first 6 months of life.MethodsA prospective cohort study was conducted on term infants born in Dr. Sardjito General Hospital and two private hospitals in Yogyakarta, Indonesia. SGA was defined as birth weight less than the 10th percentile according to Fenton criteria. Weight, length, and head circumference (HC) were measured at birth and monthly until 6 months old.ResultsA total of 39 AGA and 17 SGA term infants who were exclusively breastfed in their first 6 months were included and followed. In SGA compared to AGA, birth weight, length, and HC (mean ± SD) were significantly lower (p < 0.001). During the first 6 months, the SGAs grew in weight and length in parallel with the AGAs. At sixth months of age, the weight and length (mean ± SD) of the SGAs were significantly lower compared to the AGAs (p < 0.001). However, HC (mean ± SD) of SGAs grew significantly faster than the AGAs (p < 0.005). At sixth months of age, there were no significant differences in HC between the two groups (p = 0.824).ConclusionsIn the first 6 months, exclusively breastfed SGA term infants, in contrast to weight and length, only show catch up growth in HC, leading to HC comparable to their AGA peers at the age of 6 months.
Cell-free culture supernatants of Lactobacillus spp. and Pediococcus spp. inhibit growth of pathogenic Escherichia coli isolated from pigs in Thailand
Authors: Kaewchomphunuch Thotsapol , Charoenpichitnunt Thunyathorn , Thongbaiyai Varissara , Ngamwongsatit Natharin , Kaeoket Kampon 
Published: February 2022

Abstract
Background
Pathogenic Escherichia coli (E. coli) is an important causative agent for infectious diseases in pigs and causes significant economic loss. The global concern of antimicrobial resistance of bacteria raises awareness of the alternative ways of using antimicrobial peptides (AMPs). The study was aimed to identify and test the efficacy of AMPs from Lactobacillus spp. against the growth of pathogenic E. coli isolated from pigs in Thailand. Briefly, cell-free culture supernatants (CFCS) from 3 strains of lactic acid bacteria (LAB) consisting of Lactobacillus acidophilus (strain KMP), Lactobacillus plantarum (strain KMP), and Pediococcus pentosaceus (strain KMP) were tested against pathogenic E. coli via agar well diffusion assay in quadruplicates. The presence of a zone of inhibition (ZOI) around wells was evaluated at different incubation time. Acid and bile tolerance test was performed for bacterial viability in acid and bile salt conditions. In addition, LAB cross-streaking assay was evaluated for antagonist activity.

Results
The study showed that CFCS from L. acidophilus KMP, L. plantarum KMP, and P. pentosaceus KMP could inhibit the growth of pathogenic E. coli isolated from pigs in a time-dependent manner. To exemplify, the ZOI of L. plantarum KMP against E. coli (ETEC) at 8, 10, 12, 14, and 16 h incubation, were 26.6 ± 1.1, 24.9 ± 1.9, 22.5 ± 2.4, 20.3 ± 2.9, and 17.9 ± 3.3 mm, respectively. The ZOI was significantly different between 8, 10, 12, 14 h incubation, and the ZOI of the CFCS from L. plantarum KMP was larger than others (P-value < 0.05). Furthermore, L. acidophilus KMP, L. plantarum KMP, and P. pentosaceus KMP showed viability in pH 3.0, 0.3, and 0.5% (w/v) bile salt concentration. They exhibited no antagonist activity among each other.

Conclusions
According to the results, the CFCS from LAB including L. acidophilus KMP, L. plantarum KMP and P. pentosaceus KMP can inhibit the growth of pathogenic E. coli, isolated from pigs in Thailand. The antimicrobial activity observed was incubation time dependent.
Pyrroline-5-Carboxylate Reductase 1: a novel target for sensitizing multiple myeloma cells to bortezomib by inhibition of PRAS40-mediated protein synthesis
Authors: Oudaert Inge , Satilmis Hatice , Vlummens Philip , De Brouwer Wouter , Maes Anke , Hose Dirk , De Bruyne Elke , Ghesquière Bart , Vanderkerken Karin , De Veirman Kim , Menu Eline 
Published: February 2022

Abstract
Background
Multiple myeloma (MM) remains an incurable cancer despite advances in therapy. Therefore, the search for new targets is still essential to uncover potential treatment strategies. Metabolic changes, induced by the hypoxic bone marrow, contribute to both MM cell survival and drug resistance. Pyrroline-5-carboxylate reductase 1 and 2 (PYCR1 and PYCR2) are two mitochondrial enzymes that facilitate the last step in the glutamine-to-proline conversion. Overexpression of PYCR1 is involved in progression of several cancers, however, its’ role in hematological cancers is unknown. In this study, we investigated whether PYCR affects MM viability, proliferation and response to bortezomib.

Methods
Correlation of PYCR1/2 with overall survival was investigated in the MMRF CoMMpass trial (653 patients). OPM-2 and RPMI-8226 MM cell lines were used to perform in vitro experiments. RPMI-8226 cells were supplemented with 13C-glutamine for 48 h in both normoxia and hypoxia (< 1% O2, by chamber) to perform a tracer study. PYCR1 was inhibited by siRNA or the small molecule inhibitor pargyline. Apoptosis was measured using Annexin V and 7-AAD staining, viability by CellTiterGlo assay and proliferation by BrdU incorporation. Differential protein expression was evaluated using Western Blot. The SUnSET method was used to measure protein synthesis. All in vitro experiments were performed in hypoxic conditions.

Results
We found that PYCR1 and PYCR2 mRNA expression correlated with an inferior overall survival. MM cells from relapsed/refractory patients express significantly higher levels of PYCR1 mRNA. In line with the strong expression of PYCR1, we performed a tracer study in RPMI-8226 cells, which revealed an increased conversion of 13C-glutamine to proline in hypoxia. PYCR1 inhibition reduced MM viability and proliferation and increased apoptosis. Mechanistically, we found that PYCR1 silencing reduced protein levels of p-PRAS40, p-mTOR, p-p70, p-S6, p-4EBP1 and p-eIF4E levels, suggesting a decrease in protein synthesis, which we also confirmed in vitro. Pargyline and siPYCR1 increased bortezomib-mediated apoptosis. Finally, combination therapy of pargyline with bortezomib reduced viability in CD138+ MM cells and reduced tumor burden in the murine 5TGM1 model compared to single agents.

Conclusions
This study identifies PYCR1 as a novel target in bortezomib-based combination therapies for MM.
High expression of TRIM24 predicts worse prognosis and promotes proliferation and metastasis of epithelial ovarian cancer
Authors: Zhang Liwei , Chen Hong , Ding Baijuan , Jiang Wei 
Published: February 2022

AbstractBackgroundTripartite Motif-Containing 24 (TRIM24) is a member of the tripartite motif family. TRIM24 is claimed aberrantly activated in a number of cancers, such as breast cancer, prostate cancer and lung cancer. However, the expression of TRIM24 in epithelial ovarian cancer (EOC) and its relationship with prognosis remain unclear. In this study, we investigated the expression pattern and underlying clinical significance of TRIM24 in EOC.ResultsData from Oncomine and immunohistochemistry of tissue samples demonstrated that TRIM24 expression was obviously elevated in ovarian carcinoma compared with normal ovary tissues. Elevated TRIM24 expression was closely correlated with serum CA-125 (P = 0.0294), metastasis (P = 0.0022), FIGO (International Federation of Gynecology and Obstetrics) stage (P = 0.0068) and Ki-67 level (P = 0.0395). Kaplan–Meier survival analysis found that TRIM24 expression increased inversely with the clinical prognosis of patients with EOC. Moreover, colony formation and CCK-8 assays showed that TRIM24 promoted EOC cell growth, and tumorigenic experiments in nude mice showed that TRIM24 knockdown inhibited tumor growth in vivo. The Spearman’s correlations revealed that the expression of TRIM24 was significantly correlated with levels of Ki-67 (P = 0.01), at a correlation coefficient of 0.517. Wound-healing and transwell migration assays demonstrated TRIM24 facilitated cell migration. Mechanism studies showed that TRIM24 could promote the phosphorylation level of Akt and the process of EMT.ConclusionOur results confirmed that TRIM24 could predict poor prognosis of EOC patients and promote tumor progression by regulating Akt pathway and EMT. TRIM24 may be used as a new prognostic marker for EOC and may provide a new strategy for targeted therapy of epithelial ovarian cancer.
Glucosylceramide synthase inhibition reduces ganglioside GM3 accumulation, alleviates amyloid neuropathology, and stabilizes remote contextual memory in a mouse model of Alzheimer’s disease
Authors: Dodge James C. , Tamsett Thomas J. , Treleaven Christopher M. , Taksir Tatyana V. , Piepenhagen Peter , Sardi S. Pablo , Cheng Seng H. , Shihabuddin Lamya S. 
Published: February 2022

Abstract
Background
Gangliosides are highly enriched in the brain and are critical for its normal development and function. However, in some rare neurometabolic diseases, a deficiency in lysosomal ganglioside hydrolysis is pathogenic and leads to early-onset neurodegeneration, neuroinflammation, demyelination, and dementia. Increasing evidence also suggests that more subtle ganglioside accumulation contributes to the pathogenesis of more common neurological disorders including Alzheimer’s disease (AD). Notably, ganglioside GM3 levels are elevated in the brains of AD patients and in several mouse models of AD, and plasma GM3 levels positively correlate with disease severity in AD patients.

Methods
Tg2576 AD model mice were fed chow formulated with a small molecule inhibitor of glucosylceramide synthase (GCSi) to determine whether reducing glycosphingolipid synthesis affected aberrant GM3 accumulation, amyloid burden, and disease manifestations in cognitive impairment. GM3 was measured with LC-MS, amyloid burden with ELISA and amyloid red staining, and memory was assessed using the contextual fear chamber test.

Results
GCSi mitigated soluble Aβ42 accumulation in the brains of AD model mice when treatment was started prophylactically. Remarkably, GCSi treatment also reduced soluble Aβ42 levels and amyloid plaque burden in aged (i.e., 70 weeks old) AD mice with preexisting neuropathology. Our analysis of contextual memory in Tg2576 mice showed that impairments in remote (cortical-dependent) memory consolidation preceded deficits in short-term (hippocampal-dependent) contextual memory, which was consistent with soluble Aβ42 accumulation occurring more rapidly in the cortex of AD mice compared to the hippocampus. Notably, GCSi treatment significantly stabilized remote memory consolidation in AD mice—especially in mice with enhanced cognitive training. This finding was consistent with GCSi treatment lowering aberrant GM3 accumulation in the cortex of AD mice.

Conclusions
Collectively, our results indicate that glycosphingolipids regulated by GCS are important modulators of Aβ neuropathology and that glycosphingolipid homeostasis plays a critical role in the consolidation of remote memories.
MCGNet+: an improved motor imagery classification based on cosine similarity
Authors: Li Yan , Zhong Ning , Taniar David , Zhang Haolan 
Published: February 2022

AbstractIt has been a challenge for solving the motor imagery classification problem in the brain informatics area. Accuracy and efficiency are the major obstacles for motor imagery analysis in the past decades since the computational capability and algorithmic availability cannot satisfy complex brain signal analysis. In recent years, the rapid development of machine learning (ML) methods has empowered people to tackle the motor imagery classification problem with more efficient methods. Among various ML methods, the Graph neural networks (GNNs) method has shown its efficiency and accuracy in dealing with inter-related complex networks. The use of GNN provides new possibilities for feature extraction from brain structure connection. In this paper, we proposed a new model called MCGNet+, which improves the performance of our previous model MutualGraphNet. In this latest model, the mutual information of the input columns forms the initial adjacency matrix for the cosine similarity calculation between columns to generate a new adjacency matrix in each iteration. The dynamic adjacency matrix combined with the spatial temporal graph convolution network (ST-GCN) has better performance than the unchanged matrix model. The experimental results indicate that MCGNet+ is robust enough to learn the interpretable features and outperforms the current state-of-the-art methods.
Analysis of the variable factors affecting changes in the blood concentration of cyclosporine before and after transfusion of red blood cell concentrate
Authors: Uchida Masashi , Hanada Natsumi , Yamazaki Shingo , Takatsuka Hirokazu , Imai Chiaki , Utsumi Akari , Shiko Yuki , Kawasaki Yohei , Suzuki Takaaki , Ishii Itsuko 
Published: February 2022

AbstractBackgroundThe blood concentration of cyclosporine (CyA) is frequently elevated following the transfusion of red blood cell concentrate (RCC) to patients after allogeneic hematopoietic stem cell transplantation (HSCT). The aim of this retrospective study was to identify the variable factors affecting changes in the blood concentration of CyA before and after transfusion of RCC.MethodsWe enrolled 105 patients (age, 5–66 years) who received both CyA and transfusion after HSCT. The ratio of the measurement after transfusion to the measurement before transfusion was calculated for the hematocrit and blood concentration/dose ratio of CyA (termed the HCT ratio and the CyA ratio, respectively).ResultsThe blood concentration/dose ratio of CyA was increased after transfusion compared with before transfusion (P < 0.001). The HCT ratio was significantly correlated with the CyA ratio (P = 0.23,P < 0.001). The HCT ratio, concomitant medication that could elevate CyA concentration after RCC transfusion, and difference in the alkaline phosphatase level between before and after transfusion (ΔALP) were explanatory variables associated with the variation in the CyA ratio. There was no correlation between the CyA concentration after transfusion and the change in the estimated glomerular filtration rate.ConclusionsA change in the blood concentration/dose ratio of CyA was found to be associated with a change in the HCT, concomitant medication that could elevate CyA concentration after RCC transfusion, and ALP levels. If the HCT level rises significantly after RCC transfusion, clinicians and pharmacists should pay attention to changes in the blood CyA concentration.
A complicated case of myocardial infarction with nonobstructive coronary arteries with an underlying pheochromocytoma: a case report
Authors: Jung Sodam , Kang In Sook 
Published: February 2022

Abstract
Background
The signs and symptoms of pheochromocytoma can imitate those of many other diseases, which may result in confusion. Therefore, diagnosing and treating secondary hypertension due to pheochromocytoma in deteriorating patients becomes challenging.

Case presentation
A 63-year-old female patient presented to the emergency room with severe and progressive nausea. The initial diagnosis was an acute myocardial infarction based on ST-segment depression on electrocardiogram and elevated cardiac markers. Elective coronary angiography revealed nonobstructive coronary arteries. However, she suffered from a complicated clinical course for several weeks during her life-or-death crisis. She was subsequently diagnosed with a cerebral hemorrhage and a pheochromocytoma. It is unclear whether her initial presentation was due to the neurogenic stunned myocardium caused by a cerebral hemorrhage or type 2 myocardial infarction caused by a pheochromocytoma, or both. However, this case showed the significance of accurately diagnosing and treating underlying causes in patients presenting with myocardial infarction with nonobstructive coronary arteries. Early diagnosis and treatment of the pheochromocytoma may have prevented the complications experienced by the patient.

Conclusions
A catecholamine surge and blood pressure fluctuation caused severe complications. When a patient presents with an unusual clinical presentation, secondary hypertension due to pheochromocytoma should be suspected.
Demographics, clinical features, and prognosis of rare lymphoepithelioma-like carcinoma across different anatomic sites
Authors: Zhai Xiaoqian , Liu Jiewei , Lu Donghao , Zhou Qinghua 
Published: February 2022

Abstract
Background
Lymphoepithelioma-like carcinoma (LELC) is an unusual histological malignancy type. Due to the rarity of this disease, we used the Surveillance, Epidemiology, and End Results (SEER) database to investigate comprehensively and systematically the prognosis factor of LELC.

Methods
We identified 2079 patients diagnosed with LELC during 1973–2015 from the SEER database. LELC was classified according to the tumor site. We analyzed the clinical characteristics and estimated the hazard ratio (HR) of overall mortality of LELC at each site.

Results
The nasopharynx was the most frequent site where LELC (58%) occurred. A large percentage of nasopharyngeal and pulmonary LELC patients were of Asian descent (44.5 and 32.56%, respectively). Furthermore, the majority of LELC patients were rather young when diagnosed. However, urinary bladder LELC and digestive system LELC (mean age: 69.03 and 68.05 years, respectively) were mainly to be found in older patients. Then according to Kaplan–Meier survival analysis, we found that patients with pulmonary LELC had worse survival. After adjusting for clinical tumor characteristics, pulmonary LELC patients were at increased risk of overall mortality compared with nasopharyngeal LELC either at the localized stage (HR 3.12, 95% confidence interval [CI], 1.55–6.26. P < 0.01) or at the regional stage (HR 1.72, 95% CI 1.03–2.88 P = 0.04).

Conclusions
In conclusion, we found that urinary bladder and digestive system LELCs mainly were diagnosed in old people and different from other LELCs. Pulmonary LELC patients might have a bad prognosis. The origination site may represent a predictive factor for determining survival in patients with LELC.
Policy Brief 7: Reducing the social acceptability of wildlife trafficking through behaviour change interventions
Authors: Kassa Saba , Costa Jacopo , Lugolobi Robert , Baez Camargo Claudia 
Published: February 2022

Behaviour change interventions aimed at reducing the social acceptability of wildlife trafficking are an important part of efforts to prevent wildlife crime. This policy brief summarises lessons learned about how to develop and frame effective messages in the context of these interventions, based on field work conducted in Uganda.
A key first step is to narrowly identify the right target audience. While a general public awareness campaign may have its merits, it may be more effective to focus on those identified as most vulnerable to participating in wildlife trafficking, namely young men, those that live around wildlife trafficking hotspots and those involved in trade.
Second, it appears most promising to formulate messages that challenge narrow utilitarian perceptions of wildlife by highlighting the hidden costs of trafficking and its negative impact on the economy and the environment. Messages that focus on legal risks should showcase successes in detection and sanctions, especially in a context in which impunity is perceived to be high. Other messages that seek to challenge the overvalued benefits of engaging in wildlife trafficking in relation to wealth and social status should be carefully nuanced to avoid rejection.
Third, how we frame such messages is equally important. The research suggests that appealing to social identity and highlighting personal consequences are the most promising frames to adopt.
Overall, practitioners are advised to develop and test messages and approaches that are personal and precise.
This report was funded by PMI IMPACT, a grant award initiative of Philip Morris International (PMI). In the performance of their research, the authors maintained full independence from PMI. The views and opinions expressed in this document are those of the authors and do not necessarily reflect the views of PMI. Neither PMI, nor any of its affiliates, nor any person acting on their behalf may be held responsible for any use which may be made of the information contained herein.
THE HISTORY OF ORTHODOXY IN THE ARTICLES OF THE «YEARBOOK» OF THE STATE MUSEUM OF THE HISTORY OF RELIGION AND ATHEISM OF THE ACADEMY OF SCIENCES OF THE USSR
Authors: Pyzikov Denis D. 
Published: February 2022

Nowadays the theme of the scientist’s place in the situation of ideological restrictions and censorship is still relevant. So, from this point of view, it is very important to speak about humanities in the USSR and separate propaganda and scientific research. Despite the politicization of the discourse, not all religious studies of the Soviet period were the product of agitation and propaganda of atheism. Materials on the history of Orthodoxy in the «Yearbook» of the Museum of the History of Religion and Atheism are considered as an example of a scientific approach.
The aim of this research is to determine the main topics and directions of research on the history of Orthodoxy in articles of periodical «Yearbook» of the Museum of the History of Religion and Atheism. Being an important part of the scientific potential of the St. Petersburg school of religious studies, the «Yearbook» covered topical issues on the history of religion in articles by the classics of Russian religious studies.
The author uses the method of analytical study of sources and the method of comparative historical analysis to study the problem.
As a result, we can conclude that, 1) depending on the object of research, the articles can be divided into thematic groups, 2) and the «Yearbook» itself was the personification of the creative and scientific work of the Museum of the History of Religion and Atheism of the USSR Academy of Sciences.
This suggests that we can look at Soviet religious studies through the wall of propaganda and discover science itself.
„O powrót muzealnych kapci” czyli wskazanie wybranych możliwości reagowania przez muzea na trendy zmian w środowisku naturalnym i otoczeniu kulturowym
Authors: Barańska Katarzyna 
Published: February 2022

Opublikowany w sierpniu 2021 Raport IPCC dotyczący przyszłości zmian klimatycznych nie pozostawiają wątpliwości, że wszyscy muszą podjąć starania o odwrócenie śmiertelnych trendów. Instytucje muzealne również są współodpowiedzialne za przyszłość planety , od tego zależy także i ich racja bytu. W tekście poruszony został zaledwie wycinek działań muzealnych, które są lub mogą być podejmowane w celu uczestnictwa w ratowaniu Ziemi. Dotyczą one ostatniego i wydawałoby się najmniej ważnego z ogniw działań muzealnych – sklepików i pamiątek tam oferowanych. Wskazane zostały sposoby myślenia i organizowania sprzedaży pamiątek oraz argumenty na rzecz przeciwdziałania nadmiernemu konsumpcjonizmowi, potraktowane to zostało jako realizacja misji i istoty muzealnictwa.