bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of a new potent and long-lasting single chain peptide agonist of RXFP1 in cells and in vivo translational models
Authors: Illiano Stephane , Poirier Bruno , Minoletti Claire , Pasquier Olivier , Riva Laurence , Chenede Xavier , Menguy Isabelle , Guillotel Michel , Prigent Philippe , Claire Stéphane Le , Gillot Florence , Thill Gilbert , Lo-Presti François , Corbier Alain , Bail Jean-Christophe Le , Grailhe Patrick , Monteagudo Edith , Ingenito Raffaele , Bianchi Elisabetta , Philippo Christophe , Duclos Olivier , Mallart Sergio , Bathgate Ross , Janiak Philip 
Published: July 2022

Abstract
Despite beneficial effects in acute heart failure, recombinant relaxin-2 full therapeutic potential has been hampered by its short half-life and the need for intravenous administration limiting its use to intensive care units. A multiparametric optimization of relaxin B-chain led to the identification of single chain lipidated peptide agonists of RXFP1 like SA10SC-RLX with subcutaneous bioavailability and extended half-life. SA10SC-RLX has sub nanomolar activity on cells expressing human RXFP1 and molecular modeling associated with study of different RXFP1 mutant were used to decipher the mechanism of SA10SC-RLX interaction with RXFP1. Telemetry was performed in rat where SA10SC-RLX was able to engage RXFP1 after subcutaneous administration and this, without tachyphylaxis after repeated dosing. Renal blood flow was then used as a translational model to evaluate RXFP1 activation. SA10SC-RLX increased renal blood flow and decreased renal vascular resistance in rats as reported with relaxin in humans. In conclusion, SA10SC-RLX mimics relaxin activity in in vitro and in vivo models of acute RXFP1 engagement. SA10SC-RLX represents a new class of long-lasting RXFP1 agonist, suitable for once daily subcutaneous administration in patients and potentially paving the way to new treatments for chronic fibrotic and cardiovascular diseases.
Monopole Directional Antenna Bioinspired in Elliptical Leaf with Golden Ratio for WLAN and 4G Applications
Authors: Froes Eduarda , Junior Paulo F Silva , Santana Ewaldo E. C. , Sousa Carlos M. , Cruz Carlos A. M. , Aquino Viviani S. , Castro Luis S. O. , Pinto Mauro S. S. , Silva Paulo H. F. 
Published: July 2022

Abstract
In this work, it is proposed the development a monopole directional antenna, bioinspired in elliptical leaf, with aperture by golden ratio, for 4G band application. The shape antenna is generated by Gielis formula, built in FR4 substrate, with apertures calculated by golden ratio, for increasing the antenna perimeter, and to turner the resonance frequency. To compare the results of the bioinspired monopole on the elliptical sheet, a square-shaped monopole antenna was designed, simulated and measured, the structures were designed in the MATLAB program and the simulations were performed in the Ansys program. In the results compared between the square monopole and the bioinspired antenna in the elliptical sheet, it can be seen that the measured bioinspired antenna, compared to the square monopole, presented a bandwidth reduction of 77.27%, a more compact structure, with a reduction of 98%, covering the wireless local area network, and long-time evolution 4G at 2.5 GHz. The proposed technique uses a reflector on the ground plane, to change the parameters of the monopole planar antenna, of omnidirectional radiation pattern to a directional, maintaining the characteristics of the broadband, half-power beamwidth great than 100º, with high current density, and similar gain of a directional antenna. From the results, it has been observed that the elliptical leaf monopole antenna shows broadband characteristics, with a half-power beamwidth of 128 degrees, wideband, the bandwidth of 500 MHz, a gain of 6.28 dBi, a current density of 13.01 A/m², and circular polarization.
An Attack-Resistant Localization Algorithm with Adaptive Weights for Range-Based Wireless Sensor Networks
Authors: Wang Miao , Liu Qingshan , Xu Chentao 
Published: July 2022

Abstract
Localization is a crucial problem for robot control in the wireless sensors network with malicious nodes. In this paper, an attack-resistant localization algorithm with adaptive weight is proposed to achieve an accurate localization without any prior information of malicious anchors. The core idea of the proposed algorithm is adjusting the weights of malicious anchors based on the estimation for the attack value of malicious anchors to eliminate the influence of malicious anchors. Meanwhile, the subfunction's gradient is used to avoid the local minimum of non-convex function based on the particularity of the positioning problem. Three kinds of simulations are conducted to illustrate that the proposed algorithm can achieve an accurate localization in different types of attacks. Furthermore, two comparisons with other two localization methods are presented to show the advantage of the proposed algorithm by simulations and experiments.
Motorized 3D ultrasound enhances internal jugular vein dimension measurement on the International Space Station
Authors: ARBEILLE Philippe , Patterson Courtney , Greaves Danielle , Robertson Andrew , Hughson Richard 
Published: July 2022

Abstract
Traditional 2D and motorized 3D ultrasound were used to quantify internal jugular vein volume following a 4-hour venoconstrictive thigh cuff countermeasure for attenuating cephalad venous congestion during spaceflight. The 2D and 3D ultrasound results were not congruent as 2D ultrasound is subject to errors including angle of investigation even when performed with remote guidance. Motorized 3D ultrasound provides quantitative data on astronaut fluid shifts on the International Space Station.
Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature
Authors: Lu Fengning , Ding Lulu , Lu Jian , Zhou Xiangshan , Liu Haifeng , Ren Yanna , Cai Menghao 
Published: July 2022

Abstract
The polar psychrotrophic fungus Geomyces sp. WNF-15A can produce high-quality natural red pigment for the potential use as edible pigment. However, it shows low-temperature dependent synthesis of red pigment, which limits its large-scale industrial applications for the difficult and high-cost bioprocess control. This study aims to develop transposon-mediated mutagenesis methods to generate mutants that are able to synthesize red pigment at normal temperature. Four transposable systems, including single and dual transposable systems, were established in this fungus based on the Minos from Drosophila hydei and the Restless from Tolypocladium inflatum. A total of 23 production-dominant mutants and 12 growth-dominant mutants were thus obtained by this strategy. At 14℃, the MPS1 mutant achieved the highest level of red pigment (OD520 of 43.3), which was 78.4% higher than the wild-type. Red pigment production of MPS1 also reached the highest level (OD520 of 29.7) among all the mutants at 20℃, which increased by 128.7% compared to the wild-type. Of note, 4 mutants (MPS1, MPS3, MPS4 and MPD1) successfully synthesized red pigment at 25℃, which broke the production repression limit in the wild-type under normal temperature. Generally, the transposition of the dual transposable systems of Minos and Restless were more efficient than their single transposable systems for mutagenesis in this fungus. However, the positive mutation ratios were similar between the dual and single transposable systems for either Minos or Restless. This study provides alternative tools for genetic mutagenesis breeding of fungi from extreme environments.
Methodology of the INVestigating traIning assoCiated blasT pAthology (INVICTA) Study
Authors: Roy Michael J. , Keyser David O. , Rowe Sheilah , Hernandez Rene S. , Dovel Marcia , Romero Holland , Lee Diana , Menezes Matthew , MA Elizabeth Magee , Lai Chen , Gill J Jessica , Metzger Elizabeth , Werner J. Kent , Brungart Douglas , Nathan Dominic , Carr Walter S. , Brooks Danielle J. 
Published: July 2022

Abstract
Background: Subconcussive blast exposure during military training has been the subject of both anecdotal concerns and reports in the medical literature, but prior studies have often been small and have used inconsistent methods. Methods: This paper presents the methodology employed in INVestigating traIning assoCiated blasT pAthology (INVICTA) to assess a wide range of aspects of brain function, including immediate and delayed recall, gait and balance, audiologic and oculomotor function, cerebral blood flow, brain electrical activity and neuroimaging and blood biomarkers. Results: A number of the methods employed in INVICTA are relatively easy to reproducibly utilize, and can be completed efficiently, while other measures require greater technical expertise, take longer to complete, or may have logistical challenges.Conclusions: This presentation of methods used to assess the impact of blast exposure on the brain is intended to facilitate greater uniformity of data collection in this setting, which would enable comparison between different types of blast exposure and environmental circumstances, as well as to facilitate meta-analyses and syntheses across studies.
Synthesis, characterization, and in vitro anticancer evaluation of 1,3-bistetrahydrofuran- 2yl-5-FU as a potential agent for pancreatic cancer
Authors: Ndemazie Nkafu Bechem , Inkoom Andriana , Ebesoh Dexter , Bulusu Raviteja , Frimpong Esther , Trevino Jose , Han Bo , Zhu Xue , Agyare Edward 
Published: July 2022

Abstract
The failure of current chemotherapeutic agents for pancreatic cancer (PCa) makes it the most aggressive soft tissue tumor with a 5-year survival of slightly above 10% and is estimated to be the second leading cause of cancer death by 2030. Objective: The main aim was to synthesize, characterize and evaluate the in-vitro anticancer activity of 1,3-bistetrahydrofuran-2yl-5FU (MFU). Methods: MFU was synthesized by using 5-fluorouracil (5-FU) and tetrahydrofuran acetate, and characterized by nuclear magnetic resonance (NMR), micro-elemental analysis, high-performance liquid chromatography (HPLC), and liquid chromatography with mass spectrophotometry (LC-MS). MFU and Gemcitabine hydrochloride (GemHCl) were tested for antiproliferative activity against MiaPaca-2 and Panc-1 cell lines. Results: The half-minimum inhibitory concentration (IC50) of MFU was twice lower than that of GemHCl when used in both cell lines. MiaPaca-2 cells (MFU-IC50 =4.5±1.2μM vs. GemHCl-IC50=10.3±1.1μM); meanwhile similar trend was observed in Panc-1 cells (MFU-IC50=3.0 ±1μM vs. GemHCl-IC50=6.1±1.03μM). The MFU and GemHCl effects on 3D spheroids showed a similar trend (IC50-GemHCl=14.3±1.1μM vs. IC50-MFU =7.2±1.1μM) for MiaPaca-2 cells, and (IC50-GemHCl=16.3±1.1μM vs. IC50-MFU=9.2±1.1μM) for Panc-1 cells. MFU significantly inhibited clonogenic cell growth, and induced cell death via apoptosis. Cell cycle data showed mean PI for GemHCl (48.5 - 55.7) twice higher than MFU (24.7 to 27.9) for MiaPaca-2 cells, and similarly to Panc-1 cells. The in-vivo model showed intensely stained EGFR (stained brown) in all mice tumors, however, HER2 expression was less stained in MFU-treated tumors compared to GemHCl-treated tumors and controls. Conclusion: MFU was synthesized with high purity and may have potential anticancer activity against Pca.
Histone Deacetylase 1 inhibit primary hippocampal neuron dendritic growth and branching by regulation of neuronal MHC class Ⅰ molecules
Authors: Shen yuqing , Pei Wenqin , Sun Chen , Hu Yue , Li Ping , Shen Yi , Zhang Ying , Miao Fengqin , Zhang Jianqiong 
Published: July 2022

Abstract
Histone lysine deacetylase (HDACs) inhibitors are widely used as anti-cancer reagents and also as anti-neuropsychiatric reagents. Nonetheless, the cellular functions of different HDACs are found to be diverse and context-dependent from the results obtained from various HDAC inhibitors. Both HDAC1 and HDAC2 are expressed in hippocampal neurons at postnatal stage, but their functions could be overlapping or independently of one other. Major Histocompatibility Complex (MHC) class I molecules play significant roles in the process of establishment and modification of hippocampal synapses. However, the relationship between HDACs and MHC class Ⅰ molecules in the brain has not been reported. In this paper, the expressions of HDAC1 and HDAC2 is found out to be inversely correlated with those of MHC class Ⅰ molecules in the postnatal stages of mice hippocampus. Inhibition of both HDAC1 and HDAC2 by HDACs inhibitors upregulates MHC class Ⅰ molecules expression in primary cultured hippocampal neurons and this could be recapitulated by knockdown of HDAC1 using lentivirus delivery of HDAC1-specific shRNA. Growth and branching of dendrites in cultured hippocampal neurons are reduced by inhibition of HDAC1 and blocking with MHC class Ⅰ antibody can fully rescue the decreased dendritic growth in HDACs inhibitor treated neurons. Our data provide the first evidence that HDAC1 is a regulator that can shape the morphology of hippocampal neurons by modulating MHC class Ⅰ molecules.
Fibrinogen-like protein 2 prothrombinase is localized in platelets
Authors: Cherny Izhack , Hasin Pinhas , Philosoph Liatl Kalich , Shahal-Zimra Yael , Rabizadeh Esther 
Published: July 2022

Abstract
Background: Fibrinogen-like protein 2 (FGL2) is a serine protease capable of converting prothrombin into thrombin (i.e., prothrombinase activity) while bypassing the classic coagulation cascade. It has been reported to be expressed by mononuclear blood cells and endothelial cells. There are multiple reports that FGL2 supports tumor development and metastasis. However, in the blood, the origin or functional significance has not been established.Objective: To identify the origin of the FGL2 prothrombinase, a malignancy related enzyme, in peripheral blood cells.Methods: Peripheral blood samples were collected in complete blood count tubes. Blood cells and platelets were thoroughly washed to produce plasma-free samples. Procoagulant activity was measured in the samples' extracts using a thrombin generation test or an adjusted prothrombin time (PT) test in plasma deficient factor X. Results were further supported by confocal microscopy, immunoprecipitation, flow cytometry, enzyme-linked immunosorbent assays and specific inhibition assays. Results: The pro-coagulant activity of FGL2 was detected in purified platelets but not in white blood cells. FGL2 protein was readily detected in platelets. Quiescent platelets were shown to store FGL2 protein in an active form. Upon activation, platelets secreted the active FGL2 into the milieu. Conclusions: Active FGL2 prothrombinase is stored in platelets. This suggests the involvement of platelets in malignancies.
Comparative efficacy of ivermectin and fenbendazole against ancylostomiasis in dogs
Authors: Bhanjadeo Rojali Chandra , Patra Ramesh Chandra , Panda Dibya , Das Debiprasanna 
Published: July 2022

Abstract
The present experiment was carried ut to assess the comparative efficacy of ivermectin and fenbendazole individually for anthelmintic therapy for the hookworm infested dogs. Dogs presented to the Department of Veterinary Clinical Medicine or Veterinary Clinical Complex, Bhubaneswar were randomly screened for Ancylostoma caninum infection and the positive dogs were selected for the therapeutic trial Faecal samples were collected randomly from presented dogs immediately after defaecation or from the rectum directly using a faecal scoop. The collected sample was examined by floatation technique to detect the positive cases of Ancylostoma caninum infection. The dogs with normal clinical parameters and no eggs or ova in the faeces were included in in group 1 (n=12). Dogs with faecal sample positive for Ancylostoma caninum ova were recruited for the comparative study (n=24) which were grouped into two groups consisting of 12 dogs in each (group 2 and 3). Group 2 dogs (n=12) were treated with ivermectin at 200 µg/kg body weight once orally repeated after 15 days with proper supportive therapy each time. Group 3 (n=12) were treated with fenbendazole at 50 mg/kg body weight once orally repeated after 15 days with proper supportive therapy each time.Haematological examinations and serum biochemical tests were carried out in all groups each time on day 0, 15 and 30 of the experiment. The therapeutic efficacy of both the drugs was calculated on the basis of number of animals found free of Ancylostoma infection as determined by faecal sample examination and reduction in EPG count of the faeces of the group following the treatment. The reduction in eggs per gram (EPG) count on day 15 and day 30 was more significant in group 2 than group 3. The mean EPG count in fenbendazole-treated dogs reduced significantly to 24.17 + 11.44 on day 15 from day 0 level of 1650.00 + 247.25. The value again reduced from day 0 to become nil on day 30 .The 15th day after treatment, mean ± SE values of protein, albumin and globulin were changed to 5.63 ± 0.12, 2.64 ± 0.12 and 2.99 ± 0.15 g/dl, respectively. The 30th day after treatment the values were 6.23 ± 0.14, 3.20 ± 0.18 and 3.03 ± 0.21 g/dl, respectively. The total protein and albumin values were significantly changed by 15th day and 30th day at 1% level of significance as compared to that of day 0 in group 2 and 3 respectively.Following treatment with ivermectin, the 15th day haematological values increased significantly at 1% level (P<0.01) of significance. There was significant increase in the values at 1% level on the 30th day compared to day 0. The mean values were non-significantly comparable to the healthy control group except PCV and TEC on the 30th day.
Formation of subcellular compartments by condensation-prone protein OsJAZ2 in Oryza sativa and Nicotiana benthamiana leaf cells
Authors: Koja Yoshito , Joshima Yu , Yoritaka Yusuke , Arakawa Takuya , Go Haruka , Hakamata Nagisa , Kaseda Hinako , Hattori Tsukaho , Takeda Shin 
Published: July 2022

Abstract
Eukaryotic cells contain various membrane-less organelles, which are compartments consisting of proteinaceous condensates formed by phase separation. Such compartments are attractive for bioengineering and synthetic biology, because they can modify cellular function by the enrichment of molecules of interest and providing an orthogonal reaction system. This study reports that Oryza sativa JAZ2 protein (OsJAZ2) is an atypical jasmonate signalling regulator that can form large condensates in both the nucleus and cytosol of O. sativa cells. TIFY and Jas domains and low-complexity regions contribute to JAZ2 condensation, possibly by multivalent interaction, thus forming gel-like or solid compartments. Moreover, JAZ2 can also form liquid-like condensates when expressed in Nicotiana benthamiana cells. The recombinant JAZ2 fused to the green fluorescent protein (GFP) forms condensate in vitro, suggesting that the intermolecular interaction of JAZ2 molecules is a driving force for condensation. These results suggest the potential use of JAZ2 condensates to construct artificial membrane-less organelles in plant cells.
Impact of Rural Out-Migration on Technical Efficiency of Wheat and Teff Producers in Southern Ethiopia
Authors: Eshetu Fassil , Haji Jema , Ketema Mengistu , Mehare Abule 
Published: July 2022

Abstract

This study examined the impact of rural out-migration on the technical efficiency of producers using the new economics labor migration theory as a theoretical framework, and the multinomial endogenous switching regression as an analytical framework. Primary data were gathered from a random sample of 415 rural households in southern Ethiopia. Descriptive results showed that most of the migrants are young, better educated, unmarried, and male migrants. The mean technical efficiency of wheat and teff are 83 and 66 percent respectively. The average treatment effect on treated and untreated are − 5.51 and − 4.14 respectively for teff producers, and significant at 1 percent. The base heterogeneities for participation in rural-urban migration are 4.10 and 3.09 for wheat and teff producers respectively whereas the base heterogeneities for participation in international migration are 12.85 and 14.24 for wheat and teff producers respectively. Besides, the base heterogeneities for non-participation in international migration are 14.6 and 21.25 for wheat and teff producers respectively. Hence, rural out-migration significantly reduces crop technical efficiency, and the finding supports the lost labor hypothesis of the new economics labor migration theory. Promoting productivity, off-farm activity, and provision of public goods would reduce labor out-migration in southern Ethiopia.
Does digital financial inclusion reduce the risk of returning to poverty? Evidence from China Does digital financial inclusion reduce the risk of returning to poverty? Evidence from China
Authors: Xu Fang , Zhang Xiaoru , Zhou Di 
Published: July 2022

Abstract
Digital financial inclusion has become an important way to reduce poverty and prevent poverty return; however, few studies examine the relationship between digital financial inclusion measurement with poverty return governance. Based on data from the 2017 China Household Financial Survey, we construct a digital financial inclusion indicator for micro-households, and explore its impact on the risk of households returning to poverty and its mechanism of action. Our findings suggest that digital financial inclusion can reduce the risk of Chinese families returning to poverty, and that it has heterogeneous effects on families and regions with different characteristics. The main function is to improve household income level by promoting entrepreneurship and employment, and to improve risk resistance by enhancing household financial market participation and household asset allocation. Further analysis shows that digital financial inclusion has structural effects, nonlinear effects, and substitution effects with private lending in poverty governance. This paper has implications for understanding and improving the poverty governance effectiveness of digital financial inclusion.
Technological changes in the glazed wares of northern Tunisia in the transition from Fatimid to Zirid rule
Authors: Salinas Elena , Reynolds Paul , Pradell Trinitat 
Published: July 2022

Abstract
A representative selection of glazed ceramics recovered from medieval Islamic contexts excavated in the former Roman port of Utica (North Tunisia) has revealed new data about medieval glaze technologies in the central Mediterranean. Stratified sequences of pottery have established four main phases of occupation which span the mid-10th to the mid-11th centuries. The Utica assemblage demonstrates significant changes in the range of glazed wares in the Zirid period with respect to the late Fatimid period: whereas polychrome yellow-amber glazed ware, produced in the Kairouan region, was practically the only glazed ware supplied to Utica in the Fatimid period, an increasingly varied range of polychrome glazed wares was produced in various techniques in the late 10th century, early Zirid period. These new glazed ware types become dominant in the early 11th century. Up to six different glazing techniques have been identified, including transparent and opaque glazes with underglaze and overglaze decoration respectively. The absence of tin in all the glazed vessels sampled from Utica confirms the data obtained in a previous study from Bir Ftouha. Though it has been repeatedly stated that tin was used to produce the white background of Fatimid-Zirid glazed wares in Tunisia, this was achieved by adding quartz to the glaze. Some imports of glazed ceramics from Sicily and al-Andalus have been detected: their appearance in the early-mid 11th century is significant as seems to indicate a first stage in the re-activation of Mediterranean commerce, which increased in the course of the following centuries.