bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of the prognostic value of CD3 and CD8 cell densities analogous to the Immunoscore® by stage and location of colorectal cancer: an independent patient cohort study
Authors: Alwers Elizabeth , Kather Jakob N , Kloor Matthias , Brobeil Alexander , Tagscherer Katrin E , Roth Wilfried , Echle Amelie , Amitay Efrat L , Chang‐Claude Jenny , Brenner Hermann , Hoffmeister Michael 
Published: November 2022

AbstractIn addition to the traditional staging system in colorectal cancer (CRC), the Immunoscore® has been proposed to characterize the level of immune infiltration in tumor tissue and as a potential prognostic marker. The aim of this study was to examine and validate associations of an immune cell score analogous to the Immunoscore® with established molecular tumor markers and with CRC patient survival in a routine setting. Patients from a population‐based cohort study with available CRC tumor tissue blocks were included in this analysis. CD3+ and CD8+ tumor infiltrating lymphocytes in the tumor center and invasive margin were determined in stained tumor tissue slides. Based on the T‐cell density in each region, an immune cell score closely analogous to the concept of the Immunoscore® was calculated and tumors categorized into IS‐low, IS‐intermediate, or IS‐high. Logistic regression models were used to assess associations between clinicopathological characteristics with the immune cell score, and Cox proportional hazards models to analyze associations with cancer‐specific, relapse‐free, and overall survival. From 1,535 patients with CRC, 411 (27%) had IS‐high tumors. Microsatellite instability (MSI‐high) was strongly associated with higher immune cell score levels (p < 0.001). Stage I–III patients with IS‐high had better CRC‐specific and relapse‐free survival compared to patients with IS‐low (hazard ratio [HR] = 0.42 [0.27–0.66] and HR = 0.45 [0.31–0.67], respectively). Patients with microsatellite stable (MSS) tumors and IS‐high had better survival (HRCSS = 0.60 [0.42–0.88]) compared to MSS/IS‐low patients. In this population‐based cohort of CRC patients, the immune cell score was significantly associated with better patient survival. It was a similarly strong prognostic marker in patients with MSI‐high tumors and in the larger group of patients with MSS tumors. Additionally, this study showed that it is possible to implement an analogous immune cell score approach and validate the Immunoscore® using open source software in an academic setting. Thus, the Immunoscore® could be useful to improve the traditional staging system in colon and rectal cancer used in clinical practice.
Acute and long‐lasting effects of adolescent fluoxetine exposure on feeding behavior in Sprague–Dawley rats
Authors: Castro Alexis L. , Frankot Michelle , Moran Timothy H. , Iñiguez Sergio D. , Treesukosol Yada 
Published: November 2022

AbstractThe antidepressant medication fluoxetine (FLX) is frequently prescribed for the management of mood‐related illnesses in the adolescent population—yet its long‐term neurobehavioral consequences are not understood. To investigate how juvenile FLX exposure influences feeding behavior in adulthood, we conducted two experiments. In Experiment 1, adolescent male and female Sprague–Dawley rats were administered with 20 mg/kg/day FLX (postnatal day [PND] 35–49) and exposed to a binge access paradigm in adulthood (PND72+) to evaluate potential alterations for sweetened‐fat preference. No long‐term FLX‐induced differences in preference for sweetened fat versus chow, nor total caloric intake, were noted; however, females displayed higher preference for sweetened fat compared to males. In Experiment 2, PND35 male rats received FLX (PND35–49) and were exposed to chronic variable stress (CVS) in adulthood (PND74–88). During treatment, FLX decreased body weight and intake (meal size), but not total meal number. Also, no differences in meal pattern parameters were observed after FLX completion. Likewise, no differences in meal pattern parameters to a palatable diet (45% fat, 17% sucrose) presented from PND74 to PND88, even after CVS, were observed. Our findings indicate that juvenile FLX reduces body weight gain acutely via reduced meal size intake; however, no long‐term changes in ad libitum feeding behavior or binge access to a palatable stimulus are evident.
Climax forest has a higher soil bacterial diversity but lower soil nutrient contents than degraded forests in temperate northern China
Authors: Sui Xin , Li Mengsha , Frey Beat , Wang Mingyu , Weng Xiaohong , Wang Xin , Chen Fuyuan , Li Xianda , Du Zhong , Yang Libin , Li Mai‐He 
Published: November 2022

AbstractBacteria are a crucial component of forest soil biodiversity and play an important role in numerous ecosystem processes. Here, we studied the patterns of soil bacterial community diversity and structure in a climax forest (Larix gmelinii; LG) compared with those in degraded forest ecosystems of four forest vegetation types (BD, Betula dahurica; BP, Betula platyphylla; QM, Quercus mongolica; and LGQM, a mixed coniferous–broadleaved forest composed of Larix gmelinii and Quercus mongolica) in the Heilongjiang Zhongyangzhan Black‐billed Capercaillie Nature Reserve in northern China, using Illumina MiSeq sequencing of 16 S rRNA genes. Soil physicochemical properties (pH, soil organic carbon = SOC, total nitrogen = TN, carbon/nitrogen = C/N, total phosphorous = TP, available nitrogen = AN, available phosphorous = AP) differed significantly (p < .05) among the five forests. SOC, C/N, TP, AN, and AP were highest in QM, whereas SOC was lowest in LGQM. Soil pH was lowest in BD and highest in LGQM. α diversity was highest in LG and lowest in QM. The soil bacterial community composition in the climax forest was significantly different from that in the four degraded forests (p < .05). The dominant bacterial phyla were Acidobacteria, Proteobacteria, Verrucomicrobia, Bacteroidetes, Actinobacteria, Gemmatimonadetes, Firmicutes, Chloroflexi, and Rokubacteria. The highest relative abundances of these phyla were: 30.7% for Acidobacteria in LGQM, 42.6% for Proteobacteria in LG, 17.6% for Verrucomicrobia in BD, 5.5% for Firmicutes in BP, and 6.9% for Actinobacteria in QM. The dominant bacterial genera across the five forest vegetation types were Bryobacter and some poorly characterized taxa (e.g., Candidatus_Udaeobacter and Candidatus_Solibacter). Redundancy analysis indicated that SOC, C/N, TP, AN, and AP were the main soil physicochemical properties that shaped the bacterial communities. Our study revealed distinct bacterial diversity and composition in the climax forest compared with values in degraded forests, suggesting that the biotic and abiotic factors associated with climax ecosystems play an important role in shaping soil bacterial community structure and thus biogeochemical functions. The results of this study contribute to a deeper understanding and better predictions of the network among belowground systems and of the functions and services of degraded forests compared with climax ecosystems.
COI metabarcoding of large benthic Foraminifera: Method validation for application in ecological studies
Authors: Girard Elsa B. , Macher Jan‐Niklas , Jompa Jamaluddin , Renema Willem 
Published: November 2022

AbstractMonitoring community composition of Foraminifera (single‐celled marine protists) provides valuable insights into environmental conditions in marine ecosystems. Despite the efficiency of environmental DNA (eDNA) and bulk‐sample DNA (bulk‐DNA) metabarcoding to assess the presence of multiple taxa, this has not been straightforward for Foraminifera partially due to the high genetic variability in widely used ribosomal markers. Here, we test the correctness in retrieving foraminiferal communities by metabarcoding of mock communities, bulk‐DNA from coral reef sediment samples, and eDNA from their associated ethanol preservative using the recently sequenced cytochrome c oxidase subunit 1 (COI) marker. To assess the detection success, we compared our results with large benthic foraminiferal communities previously reported from the same sampling sites. Results from our mock communities demonstrate that all species were detected in two mock communities and all but one in the remaining four. Technical replicates were highly similar in number of reads for each assigned ASV in both the mock communities and bulk‐DNA samples. Bulk‐DNA showed a significantly higher species richness than their associated eDNA samples, and also detected additional species to what was already reported at the specific sites. Our study confirms that metabarcoding using the foraminiferal COI marker adequately retrieves the diversity and community composition of both the mock communities and the bulk‐DNA samples. With its decreased variability compared with the commonly used nuclear 18 S rRNA, the COI marker renders bulk‐DNA metabarcoding a powerful tool to assess foraminiferal community composition under the condition that the reference database is adequate to the target taxa.
A data set for pollinator diversity and their interactions with plants in the Pacific Northwest
Authors: Guzman Laura Melissa , Kelly Tyler , Elle Elizabeth 
Published: November 2022

AbstractPollinator populations have declined substantially in recent years. The resulting loss in pollination services has both ecological and economic consequences, including reductions in plant diversity and crop production and lower food security. Data sets that identify pollinators and their plant hosts are of the utmost importance for the light can shed on the main causes of pollinator declines. Here we present a data set that contains 67,954 individual pollinator records. The data were collected across the Pacific Northwest, primarily focused in British Columbia (Canada), with 182 individual sites over 11 years, between 2005 and 2017. This data set comprises multiple studies that aimed to collect information on pollinator abundance, diversity, and their interactions with plants. Overall, the data set includes 937 morphospecies (of which 482 were identified to the species level) of pollinators across 105 families, including data for bees, wasps, butterflies, moths, and flies. We also present information on the interactions of these species, with 473 species of plants. The data set is being released for noncommercial use only. Credits should be given to this paper (i.e., proper citation).
Orthogonal Arylation of a Diene‐Sulfonamide Using Cationic Transition Metal Catalysts
Authors: Sand Patrick , Schmidt Bernd 
Published: November 2022

AbstractThe regioselectivity of two mechanistically distinct alkenylation reactions catalyzed by in situ‐formed cationic transition metal complexes was studied using N‐allyl‐N‐phenylethenesulfonamide as a model compound. Orthogonal selectivity was observed for the Ru‐catalyzed C−H‐activating alkenylation with acetanilides, which occurs preferentially at the electron deficient double bond, and for a Pd‐catalyzed Heck‐type coupling with arene diazonium salts, which occurs preferentially at the more electron rich double bond of the N‐allyl substituent.
Quantitative assessment confirms deep proteome analysis by integrative top–down proteomics
Authors: Carbonara Katrina , Padula Matthew P. , Coorssen Jens R. 
Published: November 2022

AbstractThe goal of integrative top–down proteomics (i.e., two‐dimensional gel electrophoresis [2DE] coupled with liquid chromatography and tandem mass spectrometry [LC/MS/MS]) is a routine analytical approach that fully addresses the breadth and depth of proteomes. To accomplish this, there should be no addition, removal, or modification to any constituent proteoforms. To address two‐decade old claims of protein losses during front–end proteome resolution using 2DE, here we tested an alternate rehydration method for immobilized pH gradient strips prior to isoelectric focusing (IEF; i.e., faceup compared to facedown) and quantitatively assessed losses during the front–end of 2DE (rehydration and IEF). Using a well‐established high‐resolution, quantitative 2DE protocol, there were no detectable proteoform losses using the alternate faceup rehydration method. Although there is a <0.25% total loss of proteoforms during standard facedown rehydration, it is insignificant in terms of having any effect on overall proteome resolution (i.e., total spot count and total spot signal). This report is another milestone in integrative top–down proteomics, disproving long‐held dogma in the field and confirming that quantitative front–end 2DE/LC/MS/MS is currently the only method to broadly and deeply analyze proteomes by resolving their constituent proteoforms.
Sol‐gel route synthesis of high energy density Li [Li0.2Ni0.3Mn0.7] O2 cathode with controlled structure, morphology and enhanced electrochemical performance
Authors: Prettencia Leonard Joseph , Soundarrajan Elumalai , Roselin Ranjitha Mathiarasu , Kalaivani Raman , Raghu Subashchandrabose 
Published: November 2022

AbstractThe desire for long driving range and low cost of electric vehicles necessitates the use of superior rechargeable lithium batteries. These batteries with enhanced energy density addresses the demand for cutting‐edge cathode materials which can deliver amplified voltage and capacity. Lithium‐rich manganese is one among such promising cathodes for lithium‐ion batteries. In this work, three different organic acids, including oxalic (OX), tartaric (TA) and ascorbic (AS) acids were used to synthesis Li [Li0.2Ni0.3Mn0.7] O2 (LNMO) materials with three unique microstructures. Physicochemical and electrochemical characterization techniques were used to investigate a range of properties. Electrochemical investigations demonstrated regulated morphology‐enhanced electronic conductivity, increased energy density and prolonged cycle behavior. Among the three samples, AS‐LNMO unveiled a capacity of 308.02 mhAg−1 nearing the value of theoretical capacity. Whereas, TA‐LNMO exhibited a remarkable stability even after 200 cycles with capacity retention of 99.3%. With specific discharge capacities of 308.02, 278, 252, 228 and 212 mAhg−1 at 0.1C, 0.2C, 0.5C, 1C and 2C respectively, AS‐LNMO exhibited superior rate capability. Collectively, this research offers valuable insights in using complexing agents which positively impacts the morphology and electrochemical performance of LNMOs in upcoming lithium‐ion batteries.
Novel low‐grade renal spindle cell neoplasm with HEY1::NCOA2 fusion that is distinct from mesenchymal chondrosarcoma
Authors: Miller Timothy Isaac , Mantilla Jose G. , Wang Wenjing , Liu Yajuan J. , Tretiakova Maria 
Published: November 2022

AbstractHEY1‐NCOA2 fusion is most described in mesenchymal chondrosarcoma. This is the first case report of a primary renal spindle cell neoplasm of uncertain malignant potential with a HEY1::NCOA2 fusion identified by Fusionplex RNA‐sequencing that is histologically distinct from mesenchymal chondrosarcoma. The neoplasm was identified in a 33‐year‐old woman without significant past medical history who underwent partial nephrectomy for an incidentally discovered renal mass. The histologic features of the mass included spindle cells with variable cellularity and monotonous bland cytology forming vague fascicles and storiform architecture within a myxoedematous and collagenous stroma with areas of calcification. The morphologic and immunophenotypic features were not specific for any entity but were most similar to low‐grade fibromyxoid sarcoma. To date, the patient has not had recurrence, and the malignant potential of the neoplasm is uncertain.
Assessing the interaction effects of brain structure longitudinal changes and life environmental factors on depression and anxiety
Authors: Yang Xuena , Cheng Bolun , Yang Jian , Cheng Shiqiang , Pan Chuyu , Zhao Yijing , Zhang Huijie , Liu Li , Meng Peilin , Zhang Jingxi , Zhang Zhen , Li Chun'e , Chen Yujing , He Dan , Wen Yan , Jia Yumeng , Liu Huan , Zhang Feng 
Published: November 2022

AbstractDisrupted brain structures and several life environmental factors have been shown to influence depression and anxiety, but their interactions with anxiety and depression remain elusive. Genome‐wide association study datasets of 15 brain structure longitudinal changes (N = 15,640) were obtained from the published study. Genotype and phenotype‐related data of depression, anxiety, and life environmental factors (including smoking, alcohol drinking, coffee intake, maternal smoking, physical activity, vitamin D, insomnia, sleep duration, and family satisfaction) were collected from UK Biobank. We calculated the polygenic risk scores (PRS) of 15 brain structure changes and then conducted linear regression analyses to explore the interactions of brain structure changes and life environmental factors on depression and anxiety using 15 brain structure change‐related PRS, life environmental factors and interactions of them as instrumental variables, and depression score or anxiety score as outcomes. Sex stratification in all analyses was performed to reveal sex‐specific differences in the interactions. We found 14 shared interactions related to both depression and anxiety in total sample, such as alcohol drinking × cerebellum white matter 3 (WM; beta = −.003, p = .018 for depression; beta = −003, p = .008 for anxiety) and maternal smoking × nucleus accumbens 2 (beta = .088, p = .002 for depression; beta = .070, p = .008 for anxiety). We also observed sex‐specific differences in the interactions, for instance, alcohol drinking × cerebellum WM 3 was negatively associated with depression and anxiety in males (beta = −.004, p = .020 for depression; beta = −.005, p = .002 for anxiety). Our study results reveal the important interactions between brain structure changes and several life environmental factors on depression and anxiety, which may help to explore the pathogenesis of depression and anxiety.
Psychological distress as a predictor of the burden of care in family caregivers of COVID‐19 patients in Iran: A community‐based cross‐sectional study
Authors: Mirhosseini Seyedmohammad , Grimwood Samuel , Basirinezhad Mohammad Hasan , Montazeri Rasoul , Ebrahimi Hossein 
Published: November 2022

AbstractBackground and AimsCoronavirus disease 2019 (COVID‐19) has profoundly affected millions worldwide, including family caregivers of COVID‐19 patients, in terms of caregiver burden and psychological distress. The present study aimed to evaluate the care burden and psychological distress among family caregivers, caring for a family member with COVID‐19.MethodsThe present study was performed with a cross‐sectional design in the Shahroud community, northeast Iran using the convenience sampling method. A total of 297 family caregivers of COVID‐19 patients were assessed using self‐report scales including the depression, anxiety, and stress scale‐21 and the Zarit burden interview to assess the symptoms of psychological distress (depression, anxiety, and stress) and caregiver burden. Data were analyzed using descriptive and inferential statistics (multivariate regression analysis).ResultsFactors such as self‐reported symptoms of depression (β = 0.920, p < 0.001) and anxiety (β = 0.712, p < 0.02) were significantly predicted care burden. Variables such as lower levels of education of the family member and caregivers and also the relationship between the caregiver and family member were additional predictive factors that aggravated the care burden.ConclusionCaregivers of a family member with COVID‐19 suffer significant levels of care burden that also results from psychological distress such as depression and anxiety. Therefore, appropriate psychoeducational interventions are highly recommended.
Longitudinal links between expressive flexibility and friendship quality in adolescence: The moderating effect of social anxiety
Authors: Wang Yingqian , Hawk Skyler T. , Branje Susan , Van Lissa Caspar J. 
Published: November 2022

AbstractIntroductionExpressive flexibility, or the ability to both up‐ and down‐regulate emotional expressions in social interactions, is thought as an indicator and a consequence of healthy interpersonal relationships. The present longitudinal study examined bidirectional associations between expressive flexibility and friendship quality in early adolescence. Since prior research found inconsistent results regarding the adaptiveness of expressive flexibility, which indicated the necessity to consider individual variability in the process, we further tested the potential moderating effect of social anxiety in the links from expressive flexibility to friendship quality.MethodsParticipants from two junior high schools in eastern China (N = 274; 50.4% female; Mage = 13.56) were surveyed at three time points with 6‐month intervals. Expressive flexibility, friendship quality, and social anxiety were all assessed via self‐reported scales.ResultsAccording to the cross‐lagged model results, friendship quality significantly predicted increased expressive flexibility over time. Conversely, the longitudinal association from expressive flexibility to friendship quality was not significant, but the interaction between expressive flexibility and social anxiety significantly predicted later friendship quality. Further analyses via the Johnson–Neyman technique revealed that expressive flexibility only positively predicted friendship quality for adolescents with lower levels of social anxiety.ConclusionOur results suggest that expressive flexibility is not always socially adaptive, so practical interventions that aim to improve youths' social adjustment via expressive flexibility training might need to consider the role of individual characteristics.
Surfactant administration in preterm babies (28–36 weeks) with respiratory distress syndrome: LISA versus InSurE, an open‐label randomized controlled trial
Authors: Mishra Aradhana , Joshi Amol , Londhe Atul , Deshmukh Laxmikant 
Published: November 2022

AbstractIntroductionINtubate‐SURfactant‐Extubate (InSurE) approach is traditional method of surfactant delivery in preterm neonates with respiratory distress syndrome (RDS). Newer, less invasive surfactant administration (LISA) techniques lessen the need for mechanical ventilation and its adverse consequences. Evidence on the favorable effects of LISA can't be extrapolated from developed to developing countries. Aim of study is to compare the effectiveness of InSurE and LISA.ObjectivesPrimary outcome was to find need of intubation and mechanical ventilation within 72 h of birth. Neonates were followed until discharge/death for adverse events and complications.Materials and MethodsOpen‐label randomized controlled trial (RCT) was conducted at tertiary neonatal intensive care unit. Preterm neonates with diagnosis of RDS were randomized in two groups (InSurE or LISA) to receive surfactant soon after birth. Nasal intermittent positive pressure ventilation (NIPPV) was used as primary mode of respiratory support.ResultsA total of 150 neonates were analyzed (75 in each group). Insignificant statistical difference was seen in the need for intubation and mechanical ventilation within 72 h of birth between the two groups (InSurE, 30 [40%] and LISA, 30 [40%], relative risk 1.0, 95% confidence interval 0.68–1.48). Twelve percent (n = 9, LISA group) and 14.6% (n = 11 InSurE group) had adverse events during the procedure. Also, we observed insignificant statistical difference in the rates of major complications or duration of respiratory support, hospital stay, and mortality.ConclusionLISA and InSurE are equally effectiSpontaneously breathing pretermve for surfactant administration in the treatment of RDS, when NIPPV is the primary mode of respiratory support. More RCTs are required to compare the efficacy and long‐term outcomes of LISA with InSurE.