bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA CASC7 acts as a tumor suppressor in bladder cancer through modulating miR-103a-5p/TFPI2 axis.
Authors: XIAO JIANHUA , NING JIN-ZHUO , RUAN YUAN , CHENG FAN 
Published: September 2022

Abstract

Background
It was demonstrated that aberrant expression of long noncoding RNAs (LncRNAs) exerts regulatory effects on several malignancies. The lncRNA CASC7 effect on bladder cancer (BC) was not demonstrated. CASC7 role in BC was investigated in the current study.
Material and Methods
CASC7 expression was investigated. Using a luciferase reporter test, the link between CASC7, miR-103a-5p, and TFPI2 was confirmed. CCK-8 and flow cytometry assay were utilized for detecting apoptosis and cell viability. Transwell and wound healing tests were utilized for evaluating the invasion and migration cell capabilities. For analyzing the TFPI2, p-GSK-3β, β-catenin, and GSK-3β protein level, western blot was utilized.
Results
CASC7 expression greatly decreased in BC cell lines and clinical specimens. In function loss and gain assays, CASC7 prevents migration, proliferation, and invasion of the cell and induces cell death in BC cells, in accordance with research. In addition, CASC7 has been reported to enhance TFPI2 expression and negatively regulate the miR-103a-5p expression. Consequently, TFPI2 as a downstream target of miR-103a-5p and miR-103a-5p as a direct target of CASC7 were investigated. Moreover, over-expression of CASC7 markedly reduced the p-GSK-3β, β-catenin, expression. In contrast, the knockdown of CASC7 had the opposite effect.
Conclusion
Our findings showed that BC CASC7 functions as a tumor suppressor via miR-103a-5p and TFPI2. Its regulatory impact is related to the miR-103a-5p/TFPI2/Wnt/ β-catenin signaling pathway. In the BC treatment, CASC7 can serve as a biomarker or therapeutic target.
An efficient, eco-friendly combined technique of matrix solid-phase dispersion (MSPD)-dispersive liquid–liquid micro-extraction (DLLME) coupled with gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS) for determining polybrominated diphenyl ethers (PBDEs) in vegetables
Authors: Xu Sijie , Wang Junxia , Deng Dengxian , Wang Xuedong , Zhang Zhanen 
Published: September 2022

Abstract
A novel method of MSPD combined with DLLME for detecting 7 PBDEs in vegetables was developed by GC-MS/MS quantitation. Firstly, the freeze-dried vegetables were ground and blended with complex sorbents, and packed into a homemade column. PBDEs were eluted with only small amounts of organic solvent, collected and concentrated. Afterward, DLLME procedure was proceeded through addition of 5 mL of ultrapure water to the conical centrifuge tube for purification and pre-concentration. The optimal conditions in the MSPD and DLLME procedures were evaluated using single factor optimization. The results shown that sample weight was 0.2 g, a mixture of C18 and PSA was used as a composite sorbent with a dispersion ratio of 1:4, the elution solvent was a mixture of 8 mL n-hexane-dichloromethane (1:1,v/v) in MSPD process. After that, DLLME procedure was used 500 µl acetonitrile as dispersant and 35 µl carbon tetrachloride as extractant. The developed method showed a good linearity (R2 > 0.9992) between 1 and 1000 ng·g− 1 for all compounds, and matrix effects between − 3.3% and − 18.2%. Limits of detection (LODs) were between 0.08 and 1.00 ng·g− 1. Furthermore, the average recoveries of PBDEs were between 82.9% and 113.8% for all compounds, except for BDE 183 (between 58.5% and 82.5%) with the RSD of lower than 5.2%. This method proved to be saved-solvent, low-cost, eco-friendly, and efficient for the quantitative determination of PBDEs in actual vegetable samples under optimal conditions.
Common Genetic Abnormalities and Phenotypic Scoring in Saudi Patients with Acute B-Lymphoblastic Leukemia
Authors: Alenazi Maha , Alzahrani Faisal , Msmar Amir H. Msmar , Hassan Fathelrahman 
Published: September 2022

Abstract
Introduction: B-acute lymphoblastic leukemia (B-ALL) is a heterogenous disease that has diverse immature cell phenotypes early detection of high-risk groups. The aim of this study was to predictive scoring system using a fully standardized Euroflow eight-color panel.
Methods:The expression of the different CD markers involved in the B-ALL Euroflow panel was investigated by measuring their positivity, percentage, and median fluorescence intensity.
Results: CD9, CD123, and TdT were used to predict TCF3PBX1 with 80% SN and 100% SP. CD20 and CD66 were used to predict hypoploidy with 63% SN and 100% SP. As a result, no useful discriminative scoring system was developed.
Conclusion: Four scoring systems were proposed for the prediction of the most common cytogenetic abnormalities of Saudi B-ALL
Computerized Diagnosis of Polycystic Ovary Syndrome Using Machine Learning and Swarm Intelligence Techniques
Authors: R Subha , R Nayana B , Radhakrishnan Rekha , P Sumalatha 
Published: September 2022

Abstract
Polycystic Ovary Syndrome (PCOS) is one of the profound causes of infertility in women. Early detection, and treatment is essential in improving the prognosis in patients. The current conditions of fertility in India are skeptical, wherein women are at higher risk. PCOS is one of the major causes of infertility and scales upto 20% of women population in India. This requires a timely and accurate diagnosis which can be accomplished by developing automated diagnosing models. Having noted that the data to be dealt with consists of both clinical and non-clinical inputs, the effective information alone needs to be considered for the diagnosis. This necessitates an intelligent selection of features before diagnosing. Thus, swarm intelligence (SI) for feature selection and machine learning for classification is considered to develop a robust and efficient diagnostic model to detect PCOS condition. Initially, optimal features are selected using statistical approaches namely, correlation and Chi Square test and exhaustive search procedure by recursive elimination. Further, the SI algorithms, Particle Swarm Optimization (PSO) and Flashing firefly (FF) are attempted to identify the optimal number and feasible combination of features. Random forest classifier has been used in the ML model for classification. A comparative analysis of the results is discussed and validated based on the parameters accuracy of training and testing, precision, recall, F1-score, and AUC-ROC. The results reveal that ML models with different feature selection algorithms give best performance for different feature dimensions and the model with PSO based feature selection gives the highest performance with minimum feature size. Also PSO based algorithm evades the problem of redundancy in the feature subset.
Incidence and risk factors of postoperative hyperamylasemia and pancreatitis following total knee arthroplasty: a retrospective study
Authors: Liu Song , Li Fangguo , Hu Wei , Yang Qihao , Zhang Chi , Wang Zhao 
Published: September 2022

Abstract
Background
Postoperative hyperamylasemia and pancreatitis are recognized complications after abdominal and spinal surgeries. The aim of this study is to investigate the incidence and identify risk factors for postoperative hyperamylasemia and pancreatitis following total knee arthroplasty.
Methods
170 patients undergoing total knee arthroplasty were retrospectively identified from our database from January 2017 to January 2021. Patients were divided into normal and hyperamylasemia groups based on the presence of serum amylase level within or greater than the normal range. Postoperative pancreatitis was defined as serum amylase level greater than five times above the normal range. Patient demographics, perioperative parameters were investigated with student t test, chi square test and multivariate logistic regression analysis.
Results
43 patients (25.3%) exhibited postoperative hyperamylasemia while eight patients (4.7%) developed postoperative pancreatitis. One patient (0.6%) was designated as having clinical pancreatitis. More patients with Hypertriglyceridemia (HTG) were noted in hyperamylasemia group (P = 0.009) compared with normal group. Hyperamylasemia group showed higher preoperative serum amylase (74.95 vs 55.62 IU/L, P < 0.001), higher IBL (117.67 vs 77.01 mL, P = 0.040) and longer surgical duration (132.98 vs 107.01 min, P = 0.041). Multivariate logistic analysis revealed that HTG (OR = 0.189, P = 0.006), preoperative serum amylase (OR = 1.042, P < 0.001) and IBL (OR = 1.004, P = 0.022) were independent risk factors for postoperative hyperamylasemia.
Conclusions
A significant percentage of patients developed hyperamylasemia after total knee arthroplasty. Patients with HTG, higher preoperative serum amylase and higher IBL had an increased risk of developing postoperative hyperamylasemia and pancreatitis.
The effect of different iron sources on hemoglobin and myoglobin synthesis, and the key genes related to iron metabolism in skeletal muscle in the weanling piglets
Authors: Xu Qilong , Fan Jiajun , Gu Dianchao , Shi Bingzhao , Wang Dan , Zhu Huiling , Liu Jinsong , Liu Yulan , Kang Ping 
Published: September 2022

Abstract
This study was carried out to investigate the effect of different iron sources on hemoglobin and myoglobin (Mb) synthesis, and the mRNA expression of the key genes related to iron metabolism in skeletal muscle in the piglets. Forty eight piglets (Duroc × Large White × Landrace, 9.39 ± 1.55 kg, 40 ± 2 d) were assigned into four treatments including control group, a basal diet without iron supplemented in mineral premix; ferrous sulfate (FeSO4) group, the basal diet supplemented with FeSO4 to provide 100 mg supplemental Fe/kg dry matter (DM); ferrous glycinate (Fe-Gly) group, the basal diet supplemented with Fe-Gly to provide 80 mg supplemental Fe/kg DM; amino acid-Fe(II)-chelator complexes group, the basal diet supplemented with amino acid-Fe(II)-chelator complexes to provide 30 mg supplemental Fe/kg DM. There were six pens for each treatment, and each pen had two piglets. The experiment was lasted for 28 days. The results showed that different iron source supplementation increased the mean corpuscular hemoglobin in blood and Mb concentration in muscles (P < 0.05). FeSO4 and Fe-Gly supplementation increased the mean corpuscular volume and mean cell hemoglobin concentration in blood (P < 0.05). In addition, different iron source increased hephaestin expression in longissimus dorsi muscle (P < 0.1), decreased transferrin receptor 1 expression in longissimus dorsi muscle (P < 0.05), and increased lipocalin 2 expression in gastrocnemius muscle, respectively (P < 0.05). In summary, iron supplementation has positive effectiveness in myoglobin biosynthesis and can mobilize iron in muscles into the circulation regardless of iron sources.
Dynesys versus Dynamic Hybrid System in the treatment of Multi-Segmental Lumbar Degenerative Disease: a retrospective study
Authors: Zhang Xin , Xiao Xiao , Wang Hongyu , Wang Song , Yang Dazhi , Peng Songlin 
Published: September 2022

Abstract
Background

Of all the spinal diseases, lumbar degenerative disease (LDD) is a common and frequently-occurring disease and often develops into multi-segmental LDD over time. When multi-segment LDD occurs in middle-aged people who love sports, dynamic internal fixation, which can preserve the range of motion (ROM) and prevent adjacent segment degeneration (ASD), is more suitable for such people than interbody fusion. Nowadays, researchers have mainly focused on comparing the effectiveness of hybrid fixation versus rigid fusion, or Dynesys fixation versus rigid fusion, whereas Dynesys fixation and hybrid fixation have not been adequately compared in multi-segmental LDD in terms of clinical and radiographic evaluations.

Methods

We included 35 patients with multi-segmental LDD from January 2015 to August 2019, divided into Dynesys (only used Dynesys system, n = 22) and Hybrid (used Dynesys system and an intervertebral cage, n = 13) groups. Clinical outcomes were evaluated using perioperative data, Oswestry Disability Index (ODI), and Visual Analogue Scale (VAS). Radiologic evaluations included lumbar spine X-ray, MRI, and CT. Furthermore, different complications were analyzed.

Results

At the last follow-up, ODI and VAS of each group were significantly improved (p < 0.05), and the ROM of operating segments decreased. However, Dynesys group preserved a larger extent of ROM than that of Hybrid group at the final follow-up (p < 0.05). ROM of the upper adjacent segment was increased in both groups (p < 0.05), while the disc heights were significantly decreased at the final follow-up than those one-week post-surgery (p < 0.05). Besides, Dynesys group had a more obvious decrease in the disc height of dynamic segments than Hybrid group (p < 0.05). No significant difference existed in complications between both groups (p > 0. 05).

Conclusion

Dynesys and hybrid fixations can preserve ROM of stabilized segments. Dynesys fixation can preserve a larger extent of ROM than that of Hybrid fixation. However, hybrid fixation can better preserve the disc height of the non-fusion level. Overall, both surgical procedures can be employed as effective treatments for middle-aged and physically active patients with multi-segmental LDD.
Effect of Cryothermic and Radiofrequency Cox-Maze IV Ablation on Atrial Size and Function Assessed by 3D Echocardiography, A Randomized Trial. To Freeze or to Burn
Authors: Boano Gabriella , Vanky Farkas , Aneq Meriam Åström 
Published: September 2022

Abstract
BACKGROUND
Cox-maze procedures are safe methods of restoring sinus rhythm (SR) in patients with atrial fibrillation (AF), and are often performed concomitant with mitral valve (MV) surgery. Atrial linear scars are achieved using Cryothermy (Cryo) or radiofrequency (RF) techniques. It is unclear how these energy sources differ in terms of effects on late left atrial (LA) reverse remodeling. We used 3-dimensional echocardiography (3DE) to compare the impact of Cryo and RFprocedures on LA size and function one year after Cox-maze IV ablation concomitant with MV surgery.
METHODS
Seventy-two patients with MV disease and AF were randomized to Cryo (n = 35) or RF (n = 37) ablation. Another 33 patients were enrolled according to the protocol without ablation (NoMaze). All patients underwent echocardiogram before and one year after surgery. Between-group differences were evaluated using the Student’s paired t-test. Fisher’s analysis was used for categorical data.
RESULTS
Forty-two ablated patients recovered sinus rhythm (SR) one year post-surgery, and had comparable left and right systolic ventricular function and a mean LA size reduction of 23% (LA volume index decreased from 66 ± 24 to 54 ± 13 ml/m2; p = 0.004). The 3DE extracted reservoir and booster function were higher after RF (37 ± 10% vs. 26 ± 6%; p < 0.001) than Cryo ablation (18 ± 9 vs. 7 ± 4%; p < 0.001), while passive conduit function was comparable between groups (24 ± 11 vs. 20 ± 8%; p = 0.17). Compared to maze groups, patients with restored SR without ablation (7 patients) had similar LA size reduction but more preserved systolic atrial function. The level of LAVI reduction depended on AF duration. Up to 30% reduction in 60% of patients with documented AF less than 6 months compared to 11% in those with AF for more than 5 years.
CONCLUSIONS
SR restoration after mitral surgery and maze resulted in LA size reduction, irrespective of the applied energy source. Using 3DE, we demonstrated that compared to RF, Cryo produced an extended ablation area that implies LA structural remodeling affecting LA systolic function. Reduced reverse size remodeling was associated with longer AF history.
TRIAL REGISTRATION
DOI 10.1186/ISRCTN14454361.
FY-3E GNOS-R: a new illustration of global soil moisture monitoring
Authors: Yang Guanglin , Du Xiaoyong , Huang Lingyong , Bai Weihua , Sun Yueqiang , Xia Junming , Yin Cong , Huang Feixiong , Wu Xuerui , Liu Yan , Zhang Peng , Hu Xinqing , Sun Ling , Qi Chengli , Zhang Xiaoxin , Wang Jinsong 
Published: September 2022

Abstract
The Fengyun-3E (FY-3E), which is the first early-morning-orbit meteorological satellite, was launched by China on July 10, 2021. One of its 11 payloads is the GNSS Occultation Sounder II (GNOS-II), which is a combination of Global Navigation Satellite System (GNSS) Radio Occultation and GNSS reflectometry (GNSS-R) sensor. An important scientific goal of GNOS reflectometry (GNOS-R) is soil moisture detection. Different from previous GNSS-R missions, such as CYGNSS (Cyclone Global Navigation Satellite System), the coverage of FY-3E GNOS-R is pan-global (±85◦ latitude), providing an opportunity for global soil moisture estimation. Owing to data volume limitations, soil moisture retrieval using artificial intelligence is not employed; instead, soil moisture estimation is based on physical scattering models. The LAnd surface GNSS Reflection Simulator (LAGRS) is a spaceborne GNSS-R simulator designed specifically for FY-3E GNOS-R, which provides corresponding theoretical values of surface reflectivity. To ensure the accuracy of soil moisture estimation, the retrieval process includes not only calibration but also removal of surface roughness and vegetation effects. Using the LAGRS model, we obtained the calibration factor and roughness–vegetation factor, and with the mask of Fresnel reflectivity, we achieved soil moisture retrieval. During the entire retrieval process, The Soil Moisture Active Passive (SMAP) ancillary global soil moisture data were treated as reference values. A reasonable degree of consistency was found between the FY-3E GNOS-R soil moisture retrievals and the SMAP data (correlation coefficient: 0.9599, root mean square error: 0.0483 cm3/cm3). This study represents the first illustration of global soil moisture retrieval based on physical models using FY-3E GNOS-R.
A Dodgson-Hare Synthesis
Authors: Green-Armytage James 
Published: September 2022

Abstract
In 1876, Charles Dodgson (better known as Lewis Carroll) proposed a committee election procedure that chooses the Condorcet winner when one exists and otherwise eliminates candidates outside the Smith set, then allows for re-votes until a Condorcet winner emerges. The present paper discusses Dodgson’s work in the context of strategic election behavior and suggests a “Dodgson-Hare” method, a variation on Dodgson’s procedure, for use in public elections. This method allows for candidate withdrawal and employs Hare’s plurality-loser-elimination method to resolve persistent cycles. Given plausible assumptions about how candidates decide whether to withdraw when there is a cycle, Dodgson-Hare outperforms Hare, Condorcet-Hare, and 12 other voting rules in a series of spatial-model simulations that count how often each rule is vulnerable to coalitional manipulation. In the case of a one-dimensional spatial model, all coalitional voting strategies that are possible under Condorcet-Hare can be undone in Dodgson-Hare, by the withdrawal of candidates who have incentive to withdraw.
JEL Classifications: D71, D72
Body dissatisfaction and social anxiety among adolescents: A moderated mediation model of feeling of inferiority, family cohesion and friendship quality
Authors: Dou Qianqian , Chang Ruosong , Xu Hui 
Published: September 2022

Abstract
Previous research has documented that body dissatisfaction is positively related to social anxiety. However, little is known about the mediating and moderating mechanisms underlying this relation. Based on ecological systems theory, this study aimed to examine the chain mediation effect of friendship quality and family cohesion in the relationship between body dissatisfaction and social anxiety among adolescents and whether this mediating process was moderated by feeling of inferiority. A total of 972 students (mean age = 15.77 years, SD = 0.856) were enrolled in this study. All participants completed questionnaires regarding adolescent body dissatisfaction, feeling of inferiority, family cohesion, friendship quality and social anxiety. Our study found that body dissatisfaction was positively associated with social anxiety. Feeling of inferiority and family cohesion were found to play a chain-mediated role in the relation between body dissatisfaction and social anxiety. In addition, friendship quality plays a moderating role in the relationship between body dissatisfaction and feeling of inferiority. According to ecological systems theory, it suggested that family cohesion and friendship quality, as important elements in the microsystem of adolescents, interacted with each other, and interacted with the core parts of the ecosystem, such as body dissatisfaction and feeling of inferiority. These effects in turn have an impact on the individual's psychological characteristics-social anxiety. These findings show lights on how body dissatisfaction is associated with social anxiety in adolescents.
Moral distress in psychiatric nurses in Covid-19 crisis
Authors: tavakol nahid , molazem zahra , rakhshan mahnaz , asemani omid , bagheri shahpar 
Published: September 2022

Abstract
Background: The COVID-19 epidemic has put heavy pressure on nurses. Psychiatric nurses are also exposed to moral distress due to the special conditions of psychiatric patients and patient’s lack of cooperation in observing health protocols.
This study has been conducted to explore and describe factors that caused moral distress in psychiatric nurses during the COVID-19 epidemic.
Method: This qualitative study with a conventional content analysis approach involved 12 nurses at Shiraz University of Medical Sciences in Iran. This study was conducted in the winter of 2021. Data collection was performed by unstructured interviews, data analysis was performed based on the five steps of Graneheim and Lundman.
Results: From the data analysis, 252 initial codes were extracted, which were obtained by continuous comparison and integration of subcategories of 4 main categories including emotional responses, relational factors, Institutional factors, and management factors caused by Moral Distress in psychiatric nurses.
Conclusion: new dimensions of the causes of moral distress associated with the COVID-19 pandemic are discovered. Managers and planners should equip psychiatric hospitals with isolation facilities and Personal Protection Equipment for patients and nurses. Strengthening the ethical climate by improving communication skills and individual nursing empowerment to prevent moral distress is recommended.