bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sorting of mitochondrial and plastid heteroplasmy in Arabidopsis is extremely rapid and depends on MSH1 activity
Authors: Broz Amanda K , Keene Alexandra , Gyorfy Matheus Fernandes , Hodous Mychaela , Johnston Iain G , Sloan Daniel B 
Published: March 2022

AbstractThe fate of new mitochondrial and plastid mutations depends on their ability to persist and spread among the numerous organellar genome copies within a cell (heteroplasmy). The extent to which heteroplasmies are transmitted across generations or eliminated through genetic bottlenecks is not well understood in plants, in part because their low mutation rates make these variants so infrequent. Disruption of MutS Homolog 1 (MSH1), a gene involved in plant organellar DNA repair, results in numerous de novo point mutations, which we used to quantitatively track the inheritance of single nucleotide variants in mitochondrial and plastid genomes in Arabidopsis. We found that heteroplasmic sorting (the fixation or loss of a variant) was rapid for both organelles, greatly exceeding rates observed in animals. In msh1 mutants, plastid variants sorted faster than those in mitochondria and were typically fixed or lost within a single generation. Effective transmission bottleneck sizes for plastids and mitochondria were N ≈ 1 and 4, respectively. Restoring MSH1 function further increased the rate of heteroplasmic sorting in mitochondria (N ≈ 1.3), potentially due to its hypothesized role in promoting gene conversion as a mechanism of DNA repair, which is expected to homogenize genome copies within a cell. Heteroplasmic sorting also favored GC base pairs. Therefore, recombinational repair and gene conversion in plant organellar genomes can potentially accelerate the elimination of heteroplasmies and bias the outcome of this sorting process.Significance statementMitochondria and plastids play essential roles in eukaryotic life; thus, mutations in these organellar genomes can have severe consequences. In animals, early germline sequestration creates genetic “bottlenecks” providing cell-to-cell variance in mitochondrial mutations upon which selection can act. However, the dynamics of organellar mutations in plants and other organisms that lack early germline segregation remain unclear. Here, we show that sorting of mutations in plant organellar genomes proceeds very rapidly – much faster than in animals. In mitochondria, this process is accelerated by MSH1, a gene involved in recombination and repair of organellar genomes. This suggests that in plants, recombinational repair creates cell-to-cell variance in the frequency of organellar mutations, facilitating selection in the absence of a classical germline bottleneck.
CoCoA: Conditional Correlation Models with Association Size
Authors: Tu Danni , Mahony Bridget , Moore Tyler M. , Bertolero Maxwell A. , Alexander-Bloch Aaron F. , Gur Ruben , Bassett Dani S. , Satterthwaite Theodore D. , Raznahan Armin , Shinohara Russell T. 
Published: March 2022

SummaryMany scientific questions can be formulated as hypotheses about conditional correlations. For instance, in tests of cognitive and physical performance, the trade-off between speed and accuracy motivates study of the two variables together. A natural question is whether speed-accuracy coupling depends on other variables, such as sustained attention. Classical regression techniques, which posit models in terms of covariates and outcomes, are insufficient to investigate the effect of a third variable on the symmetric relationship between speed and accuracy. In response, we propose CoCoA (Conditional Correlation Model with Association Size), a likelihood-based statistical framework to estimate the conditional correlation between speed and accuracy as a function of additional variables. We propose novel measures of the association size, which are analogous to effect sizes on the correlation scale, while adjusting for confound variables. In simulation studies, we compare likelihood-based estimators of conditional correlation to semi-parametric estimators adapted from genome association studies, and find that the former achieves lower bias and variance under both ideal settings and model assumption misspecification. Using neurocognitive data from the Philadelphia Neurodevelopmental Cohort, we demonstrate that greater sustained attention is associated with stronger speed-accuracy coupling in a complex reasoning task while controlling for age. By highlighting conditional correlations as the outcome of interest, our model provides complementary insights to traditional regression modelling and partitioned correlation analyses.
CTLA-4 regulates PD-L1-PD-1 interactions via transendocytosis of CD80
Authors: Kennedy Alan , Robinson Max , Hinze Claudia , Waters Erin , Williams Cayman , Halliday Neil , Dovedi Simon , Sansom David M 
Published: March 2022

AbstractCTLA-4 and PD-1 are key immune checkpoints that are targeted in the treatment of cancer. Recently it has emerged that there is a physical interaction between the ligands of these pathways (CD80 and PD-L1), which can prevent PD-L1 inhibitory functions. Since CTLA-4 captures and degrades its ligands via transendocytosis, we investigated how transendocytosis of CD80 is impacted by PD-L1 interaction. We find that transendocytosis of CD80 results in a time-dependent recovery of PD-L1 availability that correlates with CD80 removal. Moreover, CD80 transendocytosis is highly specific in that only CD80 is removed, and its heterodimeric PD-L1 partner remains on the APC. We found no evidence that CTLA-4 interactions with CD80 were inhibited by PD-L1, however efficient removal of CD80 required an intact CTLA-4 cytoplasmic domain, distinguishing this process from more general trogocytosis. We also show that simple binding of CTLA-4 to the CD80-PD-L1 heterodimer is insufficient to liberate PD-L1-PD-1 interactions, suggesting that transendocytosis of CD80 is required to effectively control PD-L1-PD-1 interactions.
Sparcl1/Hevin drives inflammatory and neuropathic pain through astrocyte and NMDA receptor signaling
Authors: Chen Gang , Luo Hao , Luo Xin , Singh Sandeep K. , Ramirez Juan J. , James Michael L. , Mathew Joseph P. , Berger Miles , Eroglu Cagla , Ji Ru-Rong 
Published: March 2022

AbstractHevin/Sparcl1 is an astrocyte-secreted protein and regulates synapse formation in the brain. Here we show that astrocytic hevin signaling plays a critical role in maintaining chronic pain. Compared to wild-type mice, hevin-null mice exhibited normal mechanical and heat sensitivity but reduced inflammatory pain. Interestingly, hevin is required for the maintenance of nerve injury-induced neuropathic pain (mechanical allodynia), and hevin-null mice have faster recovery than wild-type mice from neuropathic pain after nerve injury. Intrathecal injection of wild-type hevin but not a hevin mutant that is no longer synaptogenic was sufficient to induce persistent mechanical allodynia in naïve mice and further enhanced neuropathic pain in animals with nerve injury. In hevin-null mice with nerve injury, AAV-mediated re-expression of hevin, but not mutant hevin, in GFAP-expressing spinal cord astrocytes could reinstate neuropathic pain. Mechanistically, hevin is crucial for spinal cord NMDA receptor (NMDAR) signaling, as NMDA-induced mechanical allodynia and inward currents in spinal cord lamina II neurons is reduced in hevin-null mice. Hevin potentiated NMDA currents mediated by the GluN2B-containing NMDARs. Furthermore, intrathecal injection of a neutralizing antibody against hevin alleviated acute inflammatory pain and persistent neuropathic pain. Secreted hevin was detected in mouse cerebrospinal fluid (CSF) and nerve injury significantly increased CSF hevin abundance. Finally, neurosurgery caused rapid (< 10 hours) and substantial increases (~20 fold) in HEVIN levels in human CSF. Collectively, our findings support a critical role of hevin and astrocytes in the maintenance of chronic pain. Neutralizing of secreted hevin with monoclonal antibody may provide a new therapeutic strategy for treating chronic pain and NMDAR-medicated neurodegeneration.
Increasing restaurants' sales performance: linking suppliers and chefs' culinary knowledge
Authors: Wu Shu-Hua , Ku Edward C.S. , Wu Tung-Pao 
Published: March 2022

PurposeThis research aimed to analyse the relationships between chefs' knowledge, learning with suppliers and restaurant sales performance.Design/methodology/approachA performance model was formulated, and the hypotheses were evaluated using partial least squares (PLS). The research questionnaires were sent by post to the chefs who confirmed leading for product innovation in the restaurants and yielded 166 completed questionnaires.FindingsWhilst the chef is developing new products, restaurant operations should pay attention to the supplier's subsequent income convenience. Additionally, through the cooperation of chefs and suppliers, innovative and valuable products can improve restaurant performance and maintain a competitive advantage. Moreover, tacit knowledge sharing can become a chef's ability to learn and provide competitive advantages and benefits to restaurants.Originality/valueValuable products can be advantageous to restaurants and increase restaurants' operating performance. Moreover, the development of new products is important for restaurants' operations. Further, chefs will understand market trends and make the culinary innovation process closer to the market by learning from their colleagues and partners. Additionally, chefs prefer culinary creation to provide more tacit knowledge with suppliers.
Trends and patterns in pro-environmental behaviour research: a bibliometric review and research agenda
Authors: Farrukh Muhammad , Raza Ali , Mansoor Adil , Khan Muhammad Shahid , Lee Jason Wai Chow 
Published: March 2022

PurposeThe paper aims to analyse the current trends and set the future research agenda for pro-environmental behaviour (PEB) research.Design/methodology/approachThe bibliographic data on pro-environmental behaviour research have been extracted from the Scopus database.FindingsBased on the analysis of 2,173 publications, the study presents a performance overview of PEB research in the perspective of different aspects such as the most influential publications, authors, institutes and countries. Also, the study visualises the intellectual network by mapping bibliographic coupling (BC) and co-citation.Originality/valueThe study provides a holistic view of trends and future research directions for PEB research based on performance and science mapping, which is a unique contribution to the literature.
The perceived benefits of instructional coaching for teachers
Authors: Warnock Jennifer Marie , Gibson-Sweet Monica , van Nieuwerburgh Christian J. 
Published: March 2022

PurposeCoaching for teachers has evolved as a form of professional development. The benefits of such interventions have been examined with regard to student, teacher and school outcomes, but the purpose of this paper is to present an investigation relating to the benefits of instructional coaching (Knight, 2006) to teachers as perceived by the teachers themselves.Design/methodology/approachThe study utilises a subjectivist approach with a constructivist paradigmatic stance (Morgan and Burrell, 1985). Eleven semi-structured, face-to-face interviews were conducted (King, 2019) with teachers at a secondary (high) school in the UK. The data were interpreted and analysed using thematic analysis (Braun and Clark, 2006).FindingsThe research suggests the following four benefits to the teachers who participated in the study, which aligned well with the literature review: improved relationships, awareness, reflectiveness and enhanced practice. A fifth perceived benefit reported by the participants was positive attitude, which captured a sense of increased confidence, motivation and positivity.Research limitations/implicationsFindings were based on the self-reported data of a small sample. Therefore it is not possible to generalise the findings but Thematic Analysis was used to understand perceptions and insights in a wider context (Ritchie and Lewis, 2003). This research builds on the literature focusing on perceived benefits to teachers. Findings generally support current theoretical understanding. The results of this exploration of the teacher perception of benefits of instructional coaching in one UK school contributes to the emerging literature about the benefits of coaching. The teacher perceived benefit of positive attitude does not appear to be identified in the literature.Practical implicationsIn practice, this study raised awareness of the possible benefits of coaching to educators. The authors recommend that educators, policymakers and educational leaders should further explore the potential benefits of coaching in education settings. Utilising teacher perception, the study further confirms coaching to be a useful way of supporting educators to enhance their practice.Originality/valueThis paper fulfils an identified need to gain a better understanding of teacher-perceived benefits of coaching. This understanding will support school leaders and policymakers who may be considering how to best leverage coaching in educational settings.
Study on flexural behavior of reinforced concrete beams strengthened with geopolymer adhesive bonded CFRP
Authors: Liu Jinliang , Yan Fangpu 
Published: March 2022

PurposeIn this paper, the effects of geopolymer adhesive, the number of CFRP layers and the width of pre-crack on the flexural performance of reinforced concrete beams strengthened with CFRP were studied, and the flexural capacity of strengthened beams was calculated theoretically.Design/methodology/approachReinforced concrete beams were strengthened with CFRP by geopolymer adhesive, and flexural load tests were conducted to observe the reinforcement effect. Based on the method of calculating the flexural capacity of reinforced concrete beams, a theoretical calculation model on the flexural capacity of reinforced concrete beams strengthened with geopolymer adhesive bonded CFRP was established.FindingsThe test data shown the flexural capacity of epoxy resin adhesive CFRP strengthened reinforced concrete beams is 7.76% higher than that geopolymer adhesive is used. The flexural capacity of reinforced concrete beams strengthened with three layers of CFRP is 1.86% higher than that two layers are adopted. The mean ratio of the test data and the calculation results of the flexural capacity is 0.973, and the mean square error is 0.008. It can be seen that the test data are in good agreement with the theoretical value.Originality/valueThis paper provides data support for the popularization and application of the new environment-friendly reinforcement technology, contributes to the cause of environmental protection, and provides a new method for strengthening reinforced concrete beams.
Influence of IT support on firms' cross-channel integration: the moderating role of institutional environment
Authors: Wu Xia , Li Yang , Liu Hefu , Zhang Kai 
Published: March 2022

PurposeUsing dynamic capability theory, this study investigates how information technology (IT) support affects firms' online and offline cross-channel integration (CCI). In addition, it applies institutional theory to examine how the relationships between IT support and CCI are moderated by firms' institutional environments.Design/methodology/approachA sample of 308 firms in China that conduct business in online and offline channels was empirically tested through hierarchical regression analysis.FindingsThe results showed two types of IT support facilitated CCI: IT support for strategy and IT support for process. The relationship between IT support for process and CCI was stronger than that between IT support for strategy and CCI. The results further indicated institutional environment (i.e. dysfunctional competition and government support) played differing roles in these effects, such that the relationship between IT support for strategy and CCI was significantly weakened by dysfunctional competition yet enhanced by government support. However, neither dysfunctional competition nor government support had a significant moderating role in the relationship between IT support for process and CCI.Originality/valueThis study identifies different IT support types as antecedents of CCI. It is also one of the earliest attempts to explore the influence of institutional environment on the relationship between IT support and CCI.
Impact of live commerce spillover effect on supply chain decisions
Authors: Fan Tijun , Wang Le , Song Yang 
Published: March 2022

PurposeWith the booming of live commerce, sellers provide products through not only their traditional channels but also the anchors who show products by live broadcast, forming a live commerce supply chain. In fact, such selling mode generates two effects: the live broadcast service of the anchor affects the return rate of products sold live (live commerce effect) and related products of the manufacturer sold via its own channel (live commerce spillover effect). In this paper, the authors investigate the impacts of both live commerce and live commerce spillover effect on the price decisions as well as the anchor's service effort.Design/methodology/approachThe authors establish a live commerce supply chain model where the manufacturer sells related products directly and by the anchor with a wholesale price contract. The manufacturer decides the price of product sold directly based on the anchor's broadcast effort since there exists the live commerce spillover effects. Backward induction is used to solve the Stackelberg game between the manufacturer and the anchor.FindingsThe results show that (1) the existence of the live commerce spillover effect brings more profit to the manufacturer while it reduces the anchor's profit. Moreover, the total profit of the live commerce supply chain first decreases and then increases as the intensity of the live commerce spillover effect improves. (2) The pricing of products sold directly by the manufacturer and sold through the anchor is nonmonotonic with respect to the live commerce spillover effect. (3) The increase in return cost always leads to an increase in the profit of the anchor, whether it is borne by the anchor or by the consumer. (4) If the baseline return probability is high, the anchor should increase her effort, thus securing more profit. However, the spillover effect of live commerce and the horizontal differences between products will discourage the anchor from increasing the live streaming service level.Originality/valueThe study proposes the live commerce supply chain model where the anchor balances the cost and benefit of her live broadcast effort, which lowers the consumers expected return possibility. In addition the live commerce spillover effect is introduced, reducing the expected return rate for the related products without live broadcast (in the direct channel). With the inter-influence of live commerce, the price competition between the live anchor and the manufacturer becomes more complex. By solving the typical live commerce game model, managerial insights are given for the decision makers among the live commerce supply chain.
Drivers of attitudes toward luxury and counterfeit products: the moderating role of interpersonal influence
Authors: Iyer Rajesh , Babin Barry J. , Eastman Jacqueline K. , Griffin Mitch 
Published: March 2022

PurposeThis study explores consumers' motivations to purchase luxury and counterfeit products using an international sample. In addition, it also examines the moderating role of interpersonal influence on this process. This study seeks to examine if the consumers who demand the highest quality express a preference for luxury goods over counterfeit goods.Design/methodology/approachSurvey research was employed to subjects from the USA, India, China and Russia. Responses from US and India consumers were collected using online software, whereas responses from China and Russia were collected with the help of a local market research firm.FindingsThe findings of the study indicate that consumers tend to show similar reactions based on the luxury and counterfeit consumption process examined here. In terms of interpersonal influence as a moderator, however, the study found it significantly impacts status seekers' attitude toward luxury and how a perfectionist shopper perceives counterfeit consumption.Originality/valueThis study is one of the first in the literature to empirically address both luxury and counterfeit consumption. Further it considers consumers from multiple countries with high levels of luxury good purchases.
Leveraging the balanced scorecard to reformulate the strategy of a performing arts theater: a stakeholders' perspective
Authors: Moores Sharon , Sayed Naqi , Lento Camillo , Wakil Gulraze 
Published: March 2022

PurposeThis study expands the performance management literature by developing a strategy map and balanced scorecard (BSC) for a large performing arts theater (PAT).Design/methodology/approachFirst, interviews with significant stakeholders identify key success factors (KSFs). Next, a survey is administered, and a structural model is employed to determine the importance of each KSF and their interdependent causal relationships within the PAT. Each KSF's controllability and room for improvement are also measured to facilitate implementation strategies.FindingsThe results reveal that the Financial Perspective plays a critical role in the PAT's success, while significant changes can be enacted by focusing on the Internal Processes Perspective. Regarding the individual KSF, the following emerge as the most critical: excellent reputation, attendance growth, increasing sponsorship and donation, and supporting the local arts community; however, PAT managers will have to be creative to enact change through these KSF as some are difficult to control or have little perceived room for improvement.Research limitations/implicationsThe data were collected prior to, or at the beginning of the coronavirus disease 2019 (COVID-19) pandemic. Post-pandemic priorities for the organization may have changed.Practical implicationsBy highlighting the relationships between different KSFs, this study provides PAT managers with a frame of reference for developing their BSC and performance metrics. It also offers PAT's managers a structured and adaptable approach for prioritizing their strategic choices and developing implementation plans for improved outcomes.Originality/valueThis study exemplifies the need for applied BSC studies in various sectors, including nonprofit organizations. Specifically, this study extends the performance management literature by providing an example of a large PAT's performance measures, the inter-relationships among KSF and the resulting strategy map. The results are significant because arts management is a unique discipline based upon a specific body of knowledge (Weinstein and Bukovinsky, 2009).