bluuebunny/crossref_metadata_2025_split huggingface dataset:
Addiktológiai pszichológia Magyarországon: az elmúlt 30 év áttekintése
Authors: Demetrovics Zsolt , Rácz József 
Published: February 2022

A tanulmány célja az addiktológiai szakterület elmúlt harminc-negyven évének áttekintése, a legfontosabb fejlemények, kutatási területek és eredmények összefoglaló bemutatása. A szerzők az addiktológiai pszichológia történetében három fázist azonosítanak. A 80-as évek kezdeti törekvéseit a 90-es évek közepétől a szakterületjelentős megerősödése és hazai kibontakozása követte, míg a 2000-es évek második felétől már a nemzetközi jelenlét a meghatározó. A történeti összefoglalót követően a szerzők az egyes meghatározó kutatócsoportokat és kutatási irányokat mutatják be, majd a legfontosabb szakmai közéleti fejleményeket ismertetik.The current review aims to summarise the history of the past thirty to forty years of the addiction field in Hungary and present a summary of the major developments, research fields, and scientific results. The authors identify three phases in the history of Hungarian addiction science. The initial aspirations of the 1980s were followed by a significant strengthening and development of the field.from the mid-1990s. This, however, mainly happened within Hungary, while in the next decade, from the second half of the 2000s, the international presence of the Hungarian addiction research field became more significant. Following the historical summary, the authors present the major research teams and research directions and describe the main policy developments.
SÍNDROME INFLAMATÓRIA MULTISSISTÊMICA ASSOCIADA AO SARS-CoV-2 EM CRIANÇAS E ADOLESCENTES: UMA SÉRIE DE CASOS
Authors: Albuquerque Wania Martins Freitas , Oliveira Alessandra Ribeiro Ventura 
Published: February 2022

A COVID-19, doença causada pelo SARS-CoV-2 é considerada uma crise global de saúde pública, iniciou-se em março de 2020. De acordo com as publicações iniciais, as crianças eram poupadas da COVID-19 grave, mas relatos recentes de crianças desenvolvendo a síndrome inflamatória multissistêmica, associada ao SARS-CoV-2 (MIS-C), começaram a ser publicados. O mecanismo da MIS-C ainda não está claro. É uma doença recente em crianças e adolescentes, mas sabe-se que tem uma apresentação clínica muito semelhante a outras condições inflamatórias pediátricas como a síndrome de Kawasaki, Kawasaki incompleto, síndrome do choque tóxico, sepse bacteriana e síndrome de ativação macrofágica. Neste estudo, os autores objetivam descrever as evidências clínicas e epidemiológicas encontradas sobre essa síndrome e sua associação com SARS-CoV-2, através da descrição de 7 casos de pacientes com MIS-C, acompanhados em uma unidade pediátrica. Foram descritos fatores clínicos, achados laboratoriais e tratamento para todos os pacientes e utilizados os critérios para a MIS-C, estabelecidos pela Organização Mundial de Saúde (OMS). Todos os 7 pacientes apresentaram febre, que variou de 4 a 10 dias. 4 pacientes evoluíram com choque hipotensivo. Rush cutâneo, conjuntivite, sintomas respiratórios e edema de extremidades ocorreram em 3 pacientes. Odinofagia esteve presente em dois casos. Entre os sintomas neurológicos, somente 1 paciente evoluiu com confusão mental. O estudo relatou a experiência de uma unidade de pediatria no seguimento de 7 pacientes diagnosticados com MIS-C, sendo 6 pacientes com evidências de infecção anterior por SARS-CoV-2 e sintomatologia diversa. Esses resultados apoiam esta síndrome como uma entidade clínica potencialmente impulsionada por uma resposta imunológica desordenada após infecção por SARS-CoV-2. Dados epidemiológicos e clínicos adicionais podem esclarecer a prevalência da MIS-C e sua associação com SARS-CoV-2.
Potencial reproductivo de la hembra y parámetros productivos del lechón lactante de cerdo pelón mexicano.
Authors: Angel-Hernández Arturo , García-Munguía Carlos  Alberto , García-  Munguía Alberto Margarito , Gutiérrez-Chávez Abner  Josué , Valencia-Posadas Mauricio , García-Munguía Argelia , Segura-Correa José Candelario 
Published: February 2022

El objetivo de la investigación fue conocer el comportamiento y la habilidad materna del Cerdo Pelón Mexicano (CPM) mediante el análisis de parámetros reproductivos y productivos desde el parto al destete. Se evaluaron dos ecotipos de CPM provenientes de Huehuetla, Hidalgo (CPH) y de Atlequizayán, Puebla (CPP). Las variables por lechón fueron peso al nacimiento (PN) y peso al destete (PD). En los partos no se registraron distocias, ni muertes y presentaron anidación; el ecotipo CPP presentó el mayor número en los parámetros reproductivo que CPH. Las medias de los PN y PD fueron superiores para los lechones CPH que CPP. El comportamiento materno fue favorable, ya que no se presentaron muertes al nacimiento ni en la lactancia. En los parámetros productivos las hembras del CPP tuvieron camadas ligeramente de mayor tamaño en comparación con las CPH, mientras que los lechones de CPH presentaron un mayor peso.
Tipificación y caracterización del sistema de producción del cerdo criollo de la Región Centro, México
Authors: Angel-Hernández Arturo , García-Munguía Carlos Alberto , García- Munguía Alberto Margarito , Valencia-Posadas Mauricio , Hernández Ruiz Jesús , Velázquez-Madrazo Pablo Alfonso 
Published: February 2022

El objetivo fue realizar la caracterización fenotípica y el sistema deproducción del cerdo criollo presente en la región centro de la Sierra Madre Oriental, en los municipios de Huehuetla y San Bartolo Tutotepec (Hidalgo) y Atlequizayan, Ciudad de Cuetzalan y Zacapoaxtla (Puebla). La obtención de datos se efectuó de acuerdo con la técnica de entrevista estructurada, donde se incluyeron 55 productores dispuestos a participar y que contaran con cerdos con características de cerdo pelón mexicano y cuino. Se caracterizó la morfometría de los animales, se registró el peso corporal, altura (cruz y grupa) y largo (cuerpo, trompa y cabeza) de los individuos. Se realizó un análisis factorial por componentes principales, conglomerados y jerárquico. Para las variables no paramétricas se utilizó estadísticas descriptiva y análisis de varianza multifactorial. Se identificaron dos tipos de sistemas de producción de traspatio para la cría de Cerdo Criollo en la Región Centro de la Sierra Madre Oriental, el primero es para autoconsumo, el segundo se diferencia por la incorporación de mejoras en el manejo, en la forma de producción, enfoque expansivo. Además, los cerdos criollos de la región se encuentran diferenciados en su morfometría dependiendo de su lugar de origen.
Greater Trochanteric Fracture with Lesser Trochanter Extension
Authors: Yukata Kiminori , Uchiyama Yuya , Nishida Norihiro , Ohgi Junji , Sakai Takashi , Fujii Hiroshi 
Published: February 2022

Case:
Two patients (aged 71 and 82 years) presented with a greater trochanteric fracture with lesser trochanter extension. These cases were successfully treated by prophylactic osteosynthesis to prevent secondary intertrochanteric/cervical fracture and to facilitate an early return to daily life. We also clarified the mechanical strength of the area that escaped bone fracture using the patient-specific computed tomography–based finite element method (CT/FEM).

Conclusions:
The present fractured femurs were shown to halve the axial compression strength and had only one-sixth torsional strength in patient-specific CT/FEM. These data support prophylactic surgery to prevent the secondary fractures because of this injury.
Salvage of Bone Transport with the Induced Membrane Technique in an Open Tibia Fracture with a Segmental Defect
Authors: Taha Tarek A. 
Published: February 2022

Case:
A 29-year-old man with an open tibia fracture and a 15-cm bone defect was treated with irrigation, debridement, intramedullary nailing, soft-tissue flap, and bone transport with a frame over the nail. He developed osteomyelitis of his bone transport segment close to docking, which required bone resection and an induced membrane technique to salvage his transport segment and achieve union.

Conclusions:
This report illustrates the combined use of bone transport and induced membrane technique to achieve union in a 15-cm tibial defect. It demonstrates the use of the Masquelet technique to salvage an infected bone transport segment.
Fatal Outcome After Reduction of a Superior Shoulder Dislocation, A Case Report and Literature Review
Authors: Valdivia Zúñiga Carlos Alberto , Fulvi Alessandro Nilo 
Published: February 2022

Case:
An 81-year-old woman presented a superior glenohumeral dislocation after a low-energy fall, with a mechanism of axial load while the shoulder was in adduction and extension. After successful closed reduction, the patient presented sudden symptoms of severe vascular injury with a protruding axillary hematoma and hypovolemic shock.

Conclusion:
Superior shoulder dislocations and vascular injuries are extremely rare among shoulder dislocations, especially after low-energy trauma. Vascular injuries may result in limb compromise and death. We highlight the importance of high clinical suspicion, particularly in superior dislocations, and the importance of prompt management of vascular complications to avoid catastrophic outcomes.
Fracture of the Entire Posterior Talar Process in an Adolescent Boy
Authors: Panagopoulos Andreas , Antzoulas Panagiotis , Tatarakis Dimitris , Konstantopoulou Anna , Tagaris Giorgos 
Published: February 2022

Case report:
Fractures of the talus in adolescents are uncommon and usually involve the talar body or neck. We report a rare fracture of the entire posterior talar process in a 14-year-old boy who fell during skateboarding. He was managed with open reduction and fixation with a headless screw. He gradually regained sports activities after 4 months and had no further complaints at his last follow-up evaluation, 18 months postoperatively.

Conclusion:
In posterior talar process fractures, operative treatment can provide a good functional outcome. Patients must be followed with serial radiographs because of the high incidence of nonunion and avascular necrosis.
Chronic Wear-Induced Bearing Dislocation in a Fixed-Bearing Unicompartmental Knee Arthroplasty
Authors: Kiran Manish , Kho James , Hepburn Elizabeth , Chakraverty Julian , Davies Hywel 
Published: February 2022

Case:
We present a case of late onset, bearing wear-induced failure of locking mechanism, resulting in bearing dislocation and rapidly progressive severe metallosis in a medial fixed-bearing (FB) unicompartmental knee arthroplasty (UKA).

Conclusion:
Bearing dislocation is a rare complication of chronic wear in FB UKA, and early recognition is essential to prevent metallic component articulation-induced metallosis and catastrophic failure.
Numerical Study on Particle Transport and Placement Behaviors of Ultralow Density Particles in Fracture-Vuggy Reservoirs
Authors: Zhang Tao , Gou Haoran , Mu Kefan , Guo Jianchun , Yang Ruoyu , Li Ming 
Published: February 2022

Summary
A solid/liquid two-phase flow numerical model based on the computational fluid dynamics-discrete element method (CFD-DEM) model was established to study the transport and settlement law of ultralow-density (ULD) particles during the waterdrive channel adjustment of the Tahe carbonate fractured-vuggy reservoir. The mass, momentum, and turbulence equations of the fluid phase were established in Euler coordinates, whereas the particle motion equations were established based on Newton’s second law. The interaction between the ULD particles was described using a soft sphere model, and the water and particle phases were bidirectionally coupled. Meanwhile, virtual experiments were conducted to calibrate the contact parameters of the particles, and parallel plate experiments were performed to validate the model. Using numerical simulations of particle transport behavior in fractures, the process and characteristics of particle transport and placement in fractures are demonstrated, which can be described by the settlement profile angle and equilibrium gap height. According to parameterized simulations, the change law of the settlement profile angle and equilibrium gap height with different parameters such as particle size, pump displacement, and fracture width are demonstrated, which is helpful for the prediction of migration and accumulation of ULD particles in fracture-vuggy reservoirs.
Factors Influencing Communication Quality for Successful Fertility Preservation Counselling for Adolescent and Young Adult Cancer Patients and Their Care Givers in Korea
Authors: Shin Yoo Sub , Park Mi Na , Hahn Seung Min , Kwon Seung Yeon , Ahn Won Kee , Lyu Chuhl Joo , Han Jung Woo 
Published: February 2022

Abstract
Purpose

Fertility preservation (FP) discussion is a crucial step for adolescent and young adult (AYA) cancer patients; however, it is relatively new to Asian countries. This study highlights the quality of communication during FP discussions in Korea.
Methods

Participants were AYA patients (n = 34) and their guardians (n = 34). We described clinical pathways for FP and surveyed details on the discussion characteristics and satisfaction scales during the FP discussions in Yonsei Cancer Center, Seoul, Korea. Quality of FP discussions and the degree of satisfaction with the discussions were measured on a scale of 1–7.
Results

Of the participants, two guardians did not answer the survey. All respondents reported high overall satisfaction; however, several factors were related to low satisfaction or information quality. Regarding the type of counselors, both respondent groups reported high overall satisfaction when their counselors were physicians rather than other types of care providers. Regarding information quality, guardians who were provided with both verbal and non-verbal communication tools (pamphlets, internet resources, or others) were more satisfied with the information quality than those who were provided with only verbal communication tools. Regarding the number of discussion sessions, more than one discussion session indicated improved understanding of the FP concept, higher communication, and information quality.
Conclusion

To improve the FP process for AYA cancer patients, we need to adjust the type of counselors, number of discussion sessions, and types of information. This will be the cornerstone of effective FP communications in Korea.
A Case of PMM2-CDG Caused by an A108V Mutation Associated With a Heterozygous 70 Kilobases Deletion
Authors: Lebredonchel Elodie , Riquet A. , Neut D. , Broly F. , Matthijs G. , Klein A. , Foulquier F. 
Published: February 2022

Abstract
Background: Congenital Disorders of Glycosylation (CDG) are a large group of inherited inborn errors of metabolism with more than 140 different CDG types reported to date (1). The first characterized is also the most common, PMM2-CDG, with an autosomal recessive transmission. The PMM2 gene is encoding a phosphomannomutase. Case report: We report the case of a French child, from healthy and unrelated parents, presenting congenital ataxia with hypotonia, hyperlaxity, inverted nipples and alteration of the liver function and coagulation parameters. Transferrin isoelectrofocusing revealed a typical type I CDG profile. Direct Sanger sequencing and quantitative PCR of PMM2 revealed a unique and novel genotype. On one allele, the patient was heterozygote with a known missense variant rs200203569 NM_000303.3(PMM2):c.323C>T, p.Ala108Val in exon 4. On the second allele, WGS confirmed the presence of a novel heterozygous 70453 bp deletion encompassing 6 exons of PMM2 at position chr16:8,897,826-8,968,278. Conclusion: As the maximum length of a PMM2 deletion reported by HGMS database is 28 kb, we identified the largest heterozygous deletion reported so far. The observation reveals the impact of a thorough diagnostic testing on genetic counselling: hasty conclusion of homozygosity in the case of a relatively rare variant found in an index patient with unrelated parents can be avoided using whole genome sequencing.
Repression of HIV-1 Reactivation Mediated By KRAB Fused CRISPR/dCas9 Proteins In Lymphoid And Myeloid Cell Models
Authors: Costa Lendel Correia da , Bomfim Larissa Maciel , Dittz Uilla Victoria Torres , Velozo Camila de Almeida , Cunha Rodrigo Delvecchio da , Tanuri Amilcar 
Published: February 2022

Abstract
Background: Despite antiretroviral treatment efficacy, it does not lead to the complete eradication of HIV infection. In addition, HIV-1 latency reactivation is a major challenge towards cure efforts. Two strategies to cure HIV-1 infection, the “shock and kill” is based on the eradication of the HIV-1 from the patient, and the “block and lock". The “Block and Lock” methodology aims to control HIV-1 latency reactivation, promoting a functional cure. The KRAB fused CRISPR/dCas9 (pdCas9KRAB) system was previously produced to control cell transcription. Based on this construct we developed a CRISPR RNAs (sgRNAs), to guide the pdCas9KRAB up to five different sites in HIV-1 provirus sites to block HIV-1 latency reactivation. This process was mediated by phorbol esters and HDAC inhibitors.Results: We found five sites in the HIV-1 provirus genome (LTR1-LTR5) that minimize CRISPR off-targets and transduced them in the lymphoid and myeloid HIV-1 latency models. One of the five sgRNAs (LTR5) which binds specifically in the HIV-1 LTR NFκB binding site was able to promote a robust repression of reactivation pattern in a HIV-1 latency lymphoid model stimulated with Phorbol 12-Myristate 13-Acetate (PMA) and Ingenol B (IngB), both potent protein kinase C (PKC) stimulators. Reactivation with HDAC inhibitors, such as SAHA and Panobinostat, showed the same strong inhibition of reactivation. Additionally, we observed a reduction of 100 times in HIV-1 RNA molecules, when reactivated IngB in myeloid HIV-1 latently infected U1 cells.Conclusion: Taken together, our results show that the KRAB fused CRISPR/dCas9 system can robustly prevent the HIV-1 latency reactivation process, mediated by PMA or IngB and SAHA or Panobinostat, both in myeloid and lymphoid HIV-1 latency. In addition, we demonstrated that KRAB repressor protein is crucial to reactivation resistance phenotype, and we also have shown some useful hotspots sequences in HIV-1 LTR to design sgRNAs.
Molecular Harvesting of Proteins with Electroporation In Vivo Facilitates the Profiling of Spatial Differential Protein Expression in Tumors
Authors: Vitkin Edward , Singh Amrita , Wise Julia , Ben-Elazar Shay , Yakhini Zohar , Golberg Alexander 
Published: February 2022

Abstract
Background. Excision tissue biopsy, while central to cancer treatment and precision medicine, presents risks to the patient and does not provide a sufficiently broad and faithful representation of the heterogeneity of sampled solid tumors. Methods. Here we introduce e-biopsy – a novel concept for molecular profiling of solid tumors using molecular extraction with electroporation. As e-biopsy provides access to the molecular composition of a solid tumor, it potentially facilitates tumor diagnostics without tissue resection. Furthermore, thanks to its less invasive characteristics, e-biopsy enables probing the solid tumor multiple times in several distinct locations in the same procedure, thereby enabling the spatial profiling of tumor molecular heterogeneity.Results. We demonstrate e-biopsy in vivo, using the 4T1 breast cancer model in mice to assess its performance, as well as the inferred spatial differential protein expression. In particular, we show that proteomic profiles obtained via e-biopsy in vivo distinguish the tumors from healthy tissue and reflect spatial tumor differential protein expression.Conclusions. E-biopsy provides a completely new molecular cartography modality for solid tumors, providing information that potentially enables more sensitive detection, at lesser risk, as well as more precise personalized medicine.
Root Hairs Matter at Field Scale for Maize Shoot Growth and Nutrient Uptake, But Root Trait Plasticity is Primarily Triggered by Texture and Drought
Authors: Vetterlein Doris , Phalempin Maxime , Lippold Eva , Schlüter Steffen , Schreiter Susanne , Ahmed Mutez , Carminati Andrea , Duddek Patrick , Jorda Helena , Bienert Patrick , Bienert Desiree , Tarkka Mika , Ganther Minh , Oburger Eva , Santangeli Michael , Javaux Mathieu , Vanderborgth Jan 
Published: February 2022

Abstract
Aims: Root hairs are important for uptake, especially for nutrients with low mobility in soils with high sorption capacity. Yet, this has rarely been demonstrated in the field during a whole growing season. Mutants with defective root hairs are expected to have lower nutrient uptake, unless they compensate with more root growth. Since root hairs can also contribute to the plant's water uptake their importance could change over the course of a growing season. Methods: The root hair mutant rth3 of Zea mays and the corresponding wild-type were grown for two years under field conditions on sand and loam.Results: Shoot growth and P and K uptake of the plants were promoted by the presence of hairs at all growth stages. Differences between genotypes were greater on loam than on sand until tassel emergence, because additional exploitation by hairs is more relevant in loam. Compensation for the absence of root hairs by increased root growth was not observed in absolute terms. The root to shoot ratio was higher for rth3 than for wild-type. Root traits showed high plasticity in response to texture, the most salient being a greater mean root diameter in sand, irrespective of genotype. The mechanism causing the increase in mean root diameter is still unknown. Root length density was higher in sand, which can be explained by a greater need for exploration than exploitation in this substrate. Conclusion: The greater investment in root growth on sand compared to loam is expected to alter the long-term carbon balance.