bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic clock and methylation studies in marsupials: opossums, Tasmanian devils, kangaroos, and wallabies
Authors: Horvath Steve , Haghani Amin , Zoller Joseph A. , Raj Ken , Sinha Ishani , Robeck Todd R. , Black Pete , Couzens Aidan , Lau Clive , Manoyan Meghety , Ruiz Yadiamaris Aviles , Talbott Annais , Belov Katherine , Hogg Carolyn J. , Sears Karen E. 
Published: April 2022

AbstractThe opossum (Monodelphis domestica), with its sequenced genome, ease of laboratory care and experimental manipulation, and unique biology, is the most used laboratory marsupial. Using the mammalian methylation array, we generated DNA methylation data from n = 100 opossum samples from the ear, liver, and tail. We contrasted postnatal development and later aging effects in the opossum methylome with those in mouse (Mus musculus, C57BL/6 J strain) and other marsupial species such as Tasmanian devil, kangaroos, and wallabies. While the opossum methylome is similar to that of mouse during postnatal development, it is distinct from that shared by other mammals when it comes to the age-related gain of methylation at target sites of polycomb repressive complex 2. Our immunohistochemical staining results provide additional support for the hypothesis that PRC2 activity increases with later aging in mouse tissues but remains constant in opossum tissues. We present several epigenetic clocks for opossums that are distinguished by their compatibility with tissue type (pan-tissue and blood clock) and species (opossum and human). Two dual-species human-opossum pan-tissue clocks accurately measure chronological age and relative age, respectively. The human-opossum epigenetic clocks are expected to provide a significant boost to the attractiveness of opossum as a biological model. Additional epigenetic clocks for Tasmanian devil, red kangaroos and other species of the genus Macropus may aid species conservation efforts.
Sensitivity of buildings’ carbon footprint to electricity decarbonization: a life cycle–based multi-objective optimization approach
Authors: Kiss Benedek , Szalay Zsuzsa 
Published: April 2022

Abstract
Purpose
Life cycle assessment (LCA) is a widely used method for the evaluation of buildings’ environmental impacts, but these analyses contain high levels of uncertainty. Decarbonization of electricity production is key to reach climate goals, influencing all sectors including construction The objective of this paper is to study the sensitivity of the environmentally optimum building design solution to a changing electricity mix to assist decision-making.

Methods
In this paper, multi-objective optimization was applied to minimize the life cycle global warming potential and life cycle costs of a building at the same time, using dynamic energy simulation and LCA. The variables include building envelope parameters such as window ratio, insulation type and thickness in a typical new multi-family apartment building heated with a heat pump. A static, largely fossil-based electricity mix and a dynamic, gradually decarbonizing alternative are considered, as well as two electricity price increase scenarios. New metrics have been introduced to explore the results and describe the Pareto-optimal solutions, for example the improvement potential to contextualize the achievements through the optimization.

Results and discussion
The results show that, with the current electricity mix, building envelope optimization can improve the design by 18% on average in terms of the life cycle greenhouse gas emissions compared to typical new designs and by 10% with a dynamic mix in Hungary. With today’s static electricity mix, the optimization proved that the minimum energy efficiency requirements in force are close to cost optimality. However, from an environmental point of view, much higher insulation thicknesses have been shown as justified (U-values of less than 0.1 W/m2K).

Conclusions
Optimization of building design based on LCA is still not widely applied and the effect of a decarbonizing electricity mix on the optimum has not been studied before. The results of the paper demonstrate the importance of this question. Optimal solutions are different depending on the applied mix but an energy-efficient building envelope remains important: today’s cost-optimal building envelope proves to be both cost- and environmentally optimal in the long run.
An effect factor approach for quantifying the impact of plastic additives on aquatic biota in life cycle assessment
Authors: Tang Yibo , Mankaa Rose Nangah , Traverso Marzia 
Published: April 2022

Abstract
Purpose
Plastic pervades now almost every aspect of our daily lives, but this prosperity has led to an increasing amount of plastic debris, which is now widespread in the oceans and represents a serious threat to biota. However, there is a general lack of consideration regarding marine plastic impacts in life cycle assessment (LCA). This paper presents a preliminary approach to facilitate the characterization of chemical impacts related to marine plastic within the LCA framework.

Methods
A literature review was carried out first to summarize the current state of research on the impact assessment of marine plastic. In recent years, efforts have been made to develop LCA-compliant indicators and models that address the impact of marine littering, entanglement, and ingestion. The toxicity of plastic additives to marine biota is currently a less understood impact pathway and also the focus of this study. Relevant ecotoxicity data were collected from scientific literature for a subsequent additive-specific effect factor (EF) development, which was conducted based on the USEtox approach. Extrapolation factors used for the data conversion were also extracted from reliable sources.

Results and discussion
EFs were calculated for six commonly used additives to quantify their toxicity impacts on aquatic species. Triclosan shows an extremely high level of toxicity, while bisphenol A and bisphenol F are considered less toxic according to the results. Apart from additive-specific EFs, a generic EF was also generated, along with the species sensitivity distribution (SSD) illustrating the gathered data used to calculate this EF. Further ecotoxicity data are expected to expand the coverage of additives and species for deriving more robust EFs. In addition, a better understanding of the interactive effect between polymers and additives needs to be developed.

Conclusions
This preliminary work provides a first step towards including the impact of plastic-associated chemicals in LCA. Although the toxicity of different additives to aquatic biota may vary significantly, it is recommended to consider additives within the impact assessment of marine plastic. The generic EF can be used, together with a future EF for adsorbed environmental pollutants, to fill a gap in the characterization of plastic-related impacts in LCA.
Co-occurrence probability of water balance elements in a mountain catchment on the example of the upper Nysa Kłodzka River
Authors: Perz Adam , Sobkowiak Leszek , Wrzesiński Dariusz 
Published: April 2022

AbstractConditions of the formation of key elements of the water balance, such as precipitation and runoff, and relations between them in the mountain catchment area are very complicated, conditioned both by the climatic factor and the physiographic characteristics of the catchment area. The aim of the study is to determine relations between precipitation and runoff in the Kłodzka Valley (KV) located in mountain areas of south-western Poland. Analyzes were based on precipitation in KV and discharges of the Nysa Kłodzka River and its tributaries, recorded in hydrological years 1974–2013. The bivariate Archimedean copulas were used to describe the degree of synchronicity between these variables. The study area shows a considerable variability in the conditions of transformation of precipitation into runoff. It is conditioned both by the pluvial regime and the physical-geographical characteristics of the catchment area. As a result, sub-catchments with diversified hydrological activity and their role in the formation of water resources of the entire KV were identified. Among them, the Biała Lądecka River sub-catchment was found to be the most hydrologically active, and the sub-catchment of Bystrzyca Dusznicka River the most inert, despite e.g. quite similar synchronicity of precipitation compared to the average precipitation in KV. At the same time, the KV rivers are characterized by different types of runoff regime and characteristic of the water balance structure. The methodology presented can be useful in determining dependencies between selected elements of the water balance and evaluation of water resources availability in source areas of mountain rivers.
3D subsoil reconstruction of a mud volcano in central Sicily by means of geophysical surveys
Authors: Grassi Sabrina , De Guidi Giorgio , Patti Graziano , Brighenti Fabio , Carnemolla Francesco , Imposa Sebastiano 
Published: April 2022

AbstractThe upwelling of fluids, subject to overpressure, along with discontinuities in the subsoil, causes the formation of geological structures known as mud volcanoes. These structures, very widespread in the world and in some cases located near inhabited centers, can represent a considerable risk factor for the population, as they can give rise to paroxysmal eruptions, even very violent. The assessment of the characteristics and structure of the subsoil of the areas affected by this phenomenon can prove to be a useful tool for risk mitigation. Non-invasive geophysical surveys were carried out in the area of the active cone of the Santa Barbara mud volcano in order to obtain a 3D characterization of the subsoil. Through the processing and integration of data derived from active and passive seismic surveys was obtained, a 3D model showing the seismostratigraphic subsoil structure. The electrical resistivity tomography surveys provided results comparable to those obtained from seismic surveys and supplied information on the perimetral areas of the mud volcano. The information obtained is useful to study the boundary conditions that influence short-scale activity. Furthermore, this study seeks to evaluate the possibility of using the proposed methodological approach for monitoring the variations that occur over time in the areas affected by mud volcanoes phenomenon.
Die Bedeutung des lokalen Kontexts für die soziale Teilhabe geflüchteter Frauen – Ländliche Räume als Potenzial?
Authors: Manahl Caroline 
Published: April 2022

ZusammenfassungIn rezenten Forschungsarbeiten zu Zuwanderung in ländlichen Räumen wird ruralen Gebieten ein hohes Potenzial für Eingliederungsprozesse zugeschrieben. Besonders der Aufbau sozialer Beziehungen soll Zugewanderten in solchen Gebieten leichter gelingen und wiederum für andere Bereiche von Integration förderlich sein (z. B. für die Suche nach Wohnraum bzw. nach einer Beschäftigung). Dieser Beitrag überprüft, ob sich solche positiven Effekte ländlicher Gebiete im Vergleich zu städtischen Räumen am Beispiel geflüchteter Frauen in Vorarlberg nachweisen lassen. Dazu wird eine bi- und multivariate Analyse von Befragungsdaten durchgeführt, die in der praktischen Integrationsarbeit in Vorarlberg erhoben wurden. Dabei zeigt sich, dass geflüchtete Frauen, die in ruralen Kontexten wohnen, seltener am Erwerbsleben und an sozialen Beziehungen in ihrem lokalen Umfeld teilhaben als geflüchtete Frauen im städtischen Raum. Zudem wird ersichtlich, dass Nachbarschaften, ehrenamtliche Helfer*innen und lokale Austausch- und Begegnungsangebote wichtige Gelegenheitsstrukturen für geflüchtete Frauen sind, um Kontakte aufzubauen. Nachbarschaften in städtischen Gebieten scheinen hierfür allerdings bessere Rahmenbedingungen zu bieten als Nachbarschaften im ländlichen Raum und auch der Zugang zu Begegnungsangeboten scheint geflüchteten Frauen in der Stadt eher zu gelingen.
On Peaceful Political Relations Between Two in Luce Irigaray’s Work
Authors: Carter Jennifer 
Published: April 2022

AbstractPractical political relations according to Luce Irigaray ground the possibilities for emerging to a new political epoch. She articulates that in order to move toward a more peaceful and emancipated politics, philosophers must focus more on subject-subject relations as opposed to subject-object relations. This in turn promotes the possibility of relating to a naturally and culturally different other. She also elaborates how an emancipated politics demands initially and primarily grounding subjectivity in the two, rather than in individuality or collectivity. This is in contrast to classical liberalism which conceives of a social contract between individual agents who relate through mediations of property. Her philosophy conceives of refounding politics not only in the sphere of public government, but also in the traditionally private domestic sphere, which must become politically awakened. She articulates ways of refounding the family and the couple mediated first by relations between two, and liberating the family from its primary role as the material ground of society. Irigaray suggests that political relations at all levels must be rooted in difference rather than sameness, starting with recognizing sexual difference. Establishing such conditions involves creating civil protections and rights, especially for women, and establishing contexts nurturing to desire, including cultivating sensitivity and attending to sensible differences. Liberating contemporary politics from competitiveness and violence, and providing favorable conditions for peace, demands first attending to cultivating difference and fostering relations between two.
Blood Pressure and Spot Sign in Spontaneous Supratentorial Subcortical Intracerebral Hemorrhage
Authors: Falcone Joseph A. , Lopez Alex , Stradling Dana , Yu Wengui , Chen Jefferson W. 
Published: April 2022

Abstract
Background
Spontaneous intracerebral hemorrhage is a potentially devastating cause of brain injury, often occurring secondary to hypertension. Contrast extravasation on computed tomography angiography (CTA), known as the spot sign, has been shown to predict hematoma expansion and worse outcomes. Although hypertension has been associated with an increased rate of the spot sign being present, the relationship between spot sign and blood pressure has not been fully explored.

Methods
We retrospectively analyzed data from 134 patients (40 women and 94 men, mean age 62.3 ± 15.73 years) presenting to a tertiary academic medical center with spontaneous supratentorial subcortical intracerebral hemorrhage from 1/1/2018 to 1/4/2021.

Results
A spot sign was demonstrated in images of 18 patients (13.43%) and correlated with a higher intracerebral hemorrhage score (2.61 ± 1.42 vs. 1.31 ± 1.25, p = 0.002), larger hematoma volume (53.49cm3 ± 32.08 vs. 23.45cm3 ± 25.65, p = 0.001), lower Glasgow Coma Scale on arrival (9.06 ± 4.56 vs. 11.74 ± 3.65, p = 0.027), increased risk of hematoma expansion (16.67% vs. 5.26%, p = 0.042), and need for surgical intervention (66.67% vs. 15.52%, p < 0.001). We did not see a correlation with age, sex, or underlying comorbidities. The presence of spot sign correlated with higher modified Rankin scores at discharge (4.94 ± 1.00 vs. 3.92 ± 1.64, p < 0.001). We saw significantly higher systolic blood pressure at the time of CTA in patients with a spot sign (184 mm Hg ± 43.11 vs. 153 mm Hg ± 36.99, p = 0.009) and the highest recorded blood pressure (p = 0.019), although not blood pressure on arrival (p = 0.081). Performing CTA early in the process of blood pressure lowering was associated with a spot sign (p < 0.001).

Conclusions
The presence of spot sign correlates with larger hematomas, worse outcomes, and increased surgical intervention. There is a significant association between spot sign and systolic blood pressure at the time of CTA, with the highest systolic blood pressure being recorded prior to CTA. Although the role of intensive blood pressure management in spontaneous intracerebral hemorrhage remains a subject of debate, patients with a spot sign may be a subgroup that could benefit from this.
Cerebral Blood Flow and Oxygen Delivery in Aneurysmal Subarachnoid Hemorrhage: Relation to Neurointensive Care Targets
Authors: Svedung Wettervik Teodor , Engquist Henrik , Hånell Anders , Howells Timothy , Rostami Elham , Ronne-Engström Elisabeth , Lewén Anders , Enblad Per 
Published: April 2022

Abstract
Background
The primary aim was to determine to what extent continuously monitored neurointensive care unit (neuro-ICU) targets predict cerebral blood flow (CBF) and delivery of oxygen (CDO2) after aneurysmal subarachnoid hemorrhage. The secondary aim was to determine whether CBF and CDO2 were associated with clinical outcome.

Methods
In this observational study, patients with aneurysmal subarachnoid hemorrhage treated at the neuro-ICU in Uppsala, Sweden, from 2012 to 2020 with at least one xenon-enhanced computed tomography (Xe-CT) obtained within the first 14 days post ictus were included. CBF was measured with the Xe-CT and CDO2 was calculated based on CBF and arterial oxygen content. Regional cerebral hypoperfusion was defined as CBF < 20 mL/100 g/min, and poor CDO2 was defined as CDO2 < 3.8 mL O2/100 g/min. Neuro-ICU variables including intracranial pressure (ICP), pressure reactivity index, cerebral perfusion pressure (CPP), optimal CPP, and body temperature were assessed in association with the Xe-CT. The acute phase was divided into early phase (day 1–3) and vasospasm phase (day 4–14).

Results
Of 148 patients, 27 had underwent a Xe-CT only in the early phase, 74 only in the vasospasm phase, and 47 patients in both phases. The patients exhibited cerebral hypoperfusion and poor CDO2 for medians of 15% and 30%, respectively, of the cortical brain areas in each patient. In multiple regressions, higher body temperature was associated with higher CBF and CDO2 in the early phase. In a similar regression for the vasospasm phase, younger age and longer pulse transit time (lower peripheral resistance) correlated with higher CBF and CDO2, whereas lower hematocrit only correlated with higher CBF but not with CDO2. ICP, CPP, and pressure reactivity index exhibited no independent association with CBF and CDO2. R2 of these regressions were below 0.3. Lower CBF and CDO2 in the early phase correlated with poor outcome, but this only held true for CDO2 in multiple regressions.

Conclusions
Systemic and cerebral physiological variables exhibited a modest association with CBF and CDO2. Still, cerebral hypoperfusion and low CDO2 were common and low CDO2 was associated with poor outcome. Xe-CT imaging could be useful to help detect secondary brain injury not evident by high ICP and low CPP.
Sexualisierte Grenzverletzungen durch Lehrkräfte im Sportunterricht
Authors: Wagner Ingo , Knoke Carolin 
Published: April 2022

ZusammenfassungSowohl für den Leistungs- als auch für den Breitensport haben Studien bereits Vorfälle sexualisierter Gewalt untersucht und für den Sport allgemein ein spezifisches Risikopotenzial festgestellt. Für den Sportunterricht in der Schule stellen jedoch insbesondere sexualisierte Grenzverletzungen noch ein Forschungsdesiderat dar. Anhand retrospektiver Leitfadeninterviews mit 64 ehemaligen Schüler*innen wird exploriert, welche Formen und Konstellationen sexualisierter Grenzverletzungen im Sportunterricht der Befragten durch Lehrkräfte aufgetreten sind und wie mit diesen Vorfällen umgegangen wurde. Als Ergebnisse berichteten die Befragten sexualisierte Grenzverletzungen in verschiedensten Formen, u.a. unerwünschte Berührungen, unangenehme körperliche Nähe, unangekündigtes Betreten der Umkleideräume oder auch unangemessene verbale Grenzüberschreitungen durch die Sportlehrkraft. Die betroffenen Schüler*innen befanden sich oftmals in Konstellationen ausschließlich weiblicher Gruppen oder auch speziell von der Lehrkraft ausgewählter Schülerinnen. Im Umgang mit Vorfällen fällt insbesondere ein Mangel an standardisierten Präventionsstrukturen in Bezug auf sexualisierte Grenzverletzungen im Sportunterricht auf. Außerdem beschrieben die Betroffenen oftmals ein mangelndes Bewusstsein für das Auftreten sexualisierter Grenzverletzungen. Deduzierte Möglichkeiten zur Optimierung dieser Problematik könnten eine Thematisierung sexualisierter Grenzverletzungen mit einer offenen Kommunikationskultur im Setting Schule, feste Ansprechpartner*innen für die Schüler*innen sowie eine Standardisierung der Interventionsmaßnahmen bei auftretenden Vorfällen sein, aber auch Sensibilisierungsmaßnahmen in der Aus- und Weiterbildung von Sportlehrkräften scheinen erforderlich.
Global expression profiling of CD10 + /CD19 + pre-B lymphoblasts from Hispanic B-ALL patients correlates with comparative TARGET database analysis
Authors: Castañeda-Partida Laura , Ocadiz-Delgado Rodolfo , Sánchez-López José Manuel , García-Villa Enrique , Peñaloza-González José Gabriel , Velázquez-Aviña Martha Margarita , Torres-Nava José Refugio , Martín-Trejo Jorge Alfonso , Solís-Labastida Karina , Guerra-Castillo Francisco Xavier , Bekker-Méndez Vilma Carolina , Rosales-García Víctor Hugo , Romero-Rodríguez Dámaris , Mojica-Espinoza Raúl , Mendez-Tenorio Alfonso , Ramírez-Calzada Crystel A. , Álvarez-Ríos Elízabeth , Mejía-Aranguré Juan Manuel , Gariglio Patricio 
Published: April 2022

AbstractMexico City has one of the highest incidences of acute lymphoblastic leukemia (ALL) globally, with patients showing low survival, and high relapse rates. To gain more insight into the molecular features of B-ALL in Mexican children, we isolated CD10 + /CD19 + precursor B lymphoblasts from four bone marrow and nine peripheral blood samples of B-ALL patients using a fluorescence-activated cell sorting protocol. The global gene expression profile (BM vs PB) revealed 136 differentially expressed genes; 62 were upregulated (45.6%) and 74 were downregulated (54.4%). Pearson’s correlation coefficient was calculated to determine the similarity between pre-B lymphoblast populations. We selected 26 highly significant genes and validated 21 by RT-qPCR (CNN3, STON2, CALN1, RUNX2, GADD45A, CDC45, CDC20, PLK1, AIDA, HCK, LY86, GPR65, PIK3CG, LILRB2, IL7R, TCL1A, DOCK1, HIST1H3G, PTPN14, CD72, and NT5E). The gene set enrichment analysis of the total expression matrix and the ingenuity pathway analysis of the 136 differentially expressed genes showed that the cell cycle was altered in the bone marrow with four overexpressed genes (PLK1, CDC20, CDC45, and GADD45A) and a low expression of IL7R and PIK3CG, which are involved in B cell differentiation. A comparative bioinformatics analysis of 15 bone marrow and 10 peripheral blood samples from Hispanic B-ALL patients collected by the TARGET program, corroborated the genes observed, except for PIK3CG. We conclude the Mexican and the Hispanic B-ALL patients studied present common driver alterations and histotype-specific mutations that could facilitate risk stratification and diagnostic accuracy and serve as potential therapeutic targets.
Autonomous Bus Pilot Project Testing and Demonstration using Light Rail Transit Track
Authors: Fujiwara Akimasa , Chikaraishi Makoto , Khan Diana , Ogawa Atsufumi , Suda Yoshihiro , Yamasaki Toshikazu , Nishino Takaharu , Namba Shutaro 
Published: April 2022

AbstractThe rapidly aging society of Japan requires a quality transportation system that provides new mobility services combining innovative technologies to include all residents. The present study carries out a pilot test of the world’s first connected public transport system between autonomous buses (AB) and the light rail transit (LRT). In this system, telecommunication between two modes enabled the bus to fully self-drive and pull in precisely at an LRT stop. Reporting a result of the pilot test in Hiroshima, this paper analyzes (a) the feasibility of the system based on a monitor survey and (b) public acceptance based on a resident survey of local residents.