bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pomegranate juice and punicalagin-mediated chemoprevention of hepatocellular carcinogenesis via regulating miR-21 and NF-κB-p65 in a rat model
Authors: Hussein Aya M. , El-Beih Nadia M. , Swellam Menha , El-Hussieny Enas A. 
Published: November 2022

Abstract
Background
Hepatocellular carcinoma (HCC) is the most common neoplasm among primary liver malignancies, accounting for 70%–85% of total liver cancer cases worldwide. It is also the second-leading cause of cancer-related death worldwide. Recent research has investigated naturally occurring products high in polyphenolic compounds in the regression and prevention of HCC. This study investigated the chemoprevention effects of pomegranate juice (PJ) and punicalagin (PCG) against diethylnitrosamine (DENA)-induced hepatocarcinogenesis in male albino rats.

Methods
Animals were randomized into six groups and treated for 11 weeks as follows: group 1 was a negative control group, group 2 was treated orally with 10 mL PJ per kilogram body weight (kg bw), group 3 was treated orally with 18.5 mg PCG/kg bw, and groups 4–6 were injected with an intraperitoneal dose of DENA (50 mg/kg bw) weekly beginning in the third week. Group 4 was a HCC control (DENA-treated group), group 5 was HCC + PJ, and group 6 was HCC + PCG.

Results
PJ antagonized DENA-induced elevations of ALAT, TNF-α, NF-κB-p65, GST, MDA, and NO and restored total protein, IL-10, SOD, and CAT levels. Moreover, PJ resulted in downregulation of miR-21, Bcl-2, and Bcl-XL and an upregulation of caspase-3 and Bax mRNA expressions. These chemoprevention effects of PJ also alleviated the hepatic preneoplastic lesions induced by DENA. Although PCG treatment induced some modulation in DENA-treated rats, it did not show potent chemoprevention activity and induced some side effects.

Conclusion
Both of PJ and PCG downregulated miR-21 expression and triggered apoptosis. However, PJ was more effective than pure PCG in alleviating the hepatic antioxidant defense state and the inflammatory status. So, PJ was superior in prevention of DENA-induced hepatocellular carcinogenesis in rats than pure PCG.

Graphical Abstract
Trends in evolution of the Triatomini tribe (Hemiptera, Triatominae): reproductive incompatibility between four species of geniculatus clade
Authors: dos Reis Yago Visinho , de Oliveira Jader , Madeira Fernanda Fernandez , Ravazi Amanda , de Oliveira Ana Beatriz Bortolozo , de Mello Denis Vinicius , Campos Fabricio Ferreira , de Azeredo-Oliveira Maria Tercília Vilela , da Rosa João Aristeu , Galvão Cleber , Alevi Kaio Cesar Chaboli 
Published: November 2022

Abstract
Background
The geniculatus clade, composed by the rufotuberculatus, lignarius, geniculatus and megistus groups, relates evolutionarily the species of the genus Panstrongylus and Nesotriatoma. Several studies have shown that triatomine hybrids can play an important role in the transmission of Chagas disease. Natural hybrids between species of the geniculatus clade have never been reported to our knowledge. Thus, carrying out experimental crosses between species of the geniculatus clade can help to elucidate the taxonomic issues as well as contribute to the epidemiological knowledge of this group.

Methods
Experimental crosses were carried out between species of the megistus and lignarius groups to evaluate the reproductive compatibility between them. A phylogenetic reconstruction was also performed with data available in GenBank for the species of the geniculatus clade to show the relationships among the crossed species.

Results
Phylogenetic analysis grouped the species of the geniculatus clade into four groups, as previously reported. In the interspecific crosses performed there was no hatching of eggs, demonstrating the presence of prezygotic barriers between the crossed species and confirming their specific status.

Conclusions
In contrast to the other groups of the Triatomini tribe, as well as the Rhodniini, there are prezygotic barriers that prevent the formation of hybrids between species of the megistus and lignarius groups. Thus, the geniculatus clade may represent an important evolutionary model for Triatominae, highlighting the need for further studies with greater sample efforts for this clade (grouping the 17 species of Panstrongylus and the three of Nesotriatoma).

Graphical Abstract
Apolipoprotein E imbalance in the cerebrospinal fluid of Alzheimer’s disease patients
Authors: Lennol Matthew Paul , Sánchez-Domínguez Irene , Cuchillo-Ibañez Inmaculada , Camporesi Elena , Brinkmalm Gunnar , Alcolea Daniel , Fortea Juan , Lleó Alberto , Soria Guadalupe , Aguado Fernando , Zetterberg Henrik , Blennow Kaj , Sáez-Valero Javier 
Published: November 2022

Abstract
Objective
The purpose of this study was to examine the levels of cerebrospinal fluid (CSF) apolipoprotein E (apoE) species in Alzheimer’s disease (AD) patients.

Methods
We analyzed two CSF cohorts of AD and control individuals expressing different APOE genotypes. Moreover, CSF samples from the TgF344-AD rat model were included. Samples were run in native- and SDS-PAGE under reducing or non-reducing conditions (with or without β-mercaptoethanol). Immunoprecipitation combined with mass spectrometry or western blotting analyses served to assess the identity of apoE complexes.

Results
In TgF344-AD rats expressing a unique apoE variant resembling human apoE4, a ~35-kDa apoE monomer was identified, increasing at 16.5 months compared with wild-types. In humans, apoE isoforms form disulfide-linked dimers in CSF, except apoE4, which lacks a cysteine residue. Thus, controls showed a decrease in the apoE dimer/monomer quotient in the APOE ε3/ε4 group compared with ε3/ε3 by native electrophoresis. A major contribution of dimers was found in APOE ε3/ε4 AD cases, and, unexpectedly, dimers were also found in ε4/ε4 AD cases. Under reducing conditions, two apoE monomeric glycoforms at 36 kDa and at 34 kDa were found in all human samples. In AD patients, the amount of the 34-kDa species increased, while the 36-kDa/34-kDa quotient was lower compared with controls. Interestingly, under reducing conditions, a ~100-kDa apoE complex, the identity of which was confirmed by mass spectrometry, also appeared in human AD individuals across all APOE genotypes, suggesting the occurrence of aberrantly resistant apoE aggregates. A second independent cohort of CSF samples validated these results.

Conclusion
These results indicate that despite the increase in total apoE content the apoE protein is altered in AD CSF, suggesting that function may be compromised.
Presumed Bietti crystalline dystrophy with optic nerve head drusen: a case report
Authors: Bazvand Fatemeh , Asadi Khameneh Esmaeil 
Published: November 2022

Abstract
Background
Bietti crystalline dystrophy is primarily a retinal dystrophy caused by a CYP4V2 mutation and typically presents with crystalline retinal deposits in the posterior fundus.

Case presentation
We present the case of an otherwise healthy 39-year-old Iranian woman with no family history of ocular disease who suffered with progressive vision loss that had started 2 years prior to presentation. Ocular examination revealed blurry optic nerve head margin and diffuse retinal crystalline deposit in both eyes. Spectral domain optical coherence tomography images showed retinal crystals, located mostly in outer retinal layers, with some areas of outer retinal tubulation and attenuation of outer retinal layers. Crystalline deposits were better visualized on near-infrared images as hyperreflective spots. Fundus autofluorescence images showed hyperautofluorescence areas on optic nerve head consistent with optic nerve head drusen and large hypoautofluorescence areas in posterior retina consistent with retinal pigment epithelium atrophy. Cystinosis was ruled out by blood testing.

Conclusion
Bietti crystalline dystrophy may be associated with optic nerve head drusen.
A full digital workflow to prefabricate an implant-supported interim restoration: case report and a novel technique
Authors: Fu Yu , Yin Cuilan , Li Shiqi , Li Dan , Mo Anchun 
Published: November 2022

AbstractThis article describes a case with a full digital procedure to prefabricate an implant-supported interim restoration based on the preoperative digital implant planning. A fully guided surgical template is designed and printed, and then an interim restoration is fabricated based on the planned implant position through a dental computer-aided design (CAD) software. Once the implant was placed at the predetermined position through the fully guided surgical guide, the prefabricated interim restoration could be inserted immediately after the surgery, which can guide the healing of the soft tissue and enhance the esthetic outcomes. This novel technique eliminates the conventional impression making to insert an implant-supported interim restoration immediately after the implant placement surgery, which can guide the healing of the soft tissue, minimize the chairside time and optimize the clinical workflow.
Retrospective clinical study of mandible fractures
Authors: Cha Seungjin , Park Gaeun , Lee Baek-Soo , Kwon Yong-Dae , Choi Byung-Joon , Lee Jung-Woo , Jung Junho , Ohe Jooyoung 
Published: November 2022

Abstract
Background
As society becomes more complex, the incidence of mandibular fractures is increasing. This study aimed to analyze the incidence and type and identify etiological factors of mandibular fractures to use them in future treatments.

Material and methods
Data were collected from 224 patients who visited the department of oral and maxillofacial surgery at the Kyung Hee Medical Center dental hospital during a 6-year period (2016 to 2021). A logistic regression model was used for data analysis.

Results
In a total of 224 patients, 362 fractures were appeared. The average age of the patients was 34.1 years, with the highest incidence in the 20s. And the ratio between male and female was 4.09:1. Symphysis fractures were the most prevalent of all patients (52.7%), followed by unilateral condyle (37.1%), angle (36.2%), bilateral condyle (9.4%), body (8%), and coronoid (2.2%). The most common cause of fracture was daily-life activity (57.6%), followed by violence (30.4%), traffic accidents (8.5%), and syncope (3.6%). Patients with symphysis fracture were at low risk (OR < 1) of angle, body, and unilateral condyle fractures. Similarly, patients with unilateral fracture were at low risk (OR < 1) of symphysis, angle, body, and others site fractures. In contrast, patient with bilateral condyle fracture were at high risk (OR > 1) of coronoid fractures. And younger patients were high risk of mandibular angle fractures.

Conclusion
Through this study, it was confirmed that etiological factors of mandibular fractures were like those of previous studies.
Mesenchymal stromal cells in the thymus
Authors: Nitta Takeshi 
Published: November 2022

AbstractThe microenvironment of the thymus is composed of a group of stromal cells that include endoderm-derived thymic epithelial cells (TECs) and mesenchymal stromal cells such as fibroblasts and serves as a site for the development of T cells. TECs are known to play an essential role in T cell differentiation and selection. Mesenchymal stromal cells have been less studied in terms of their immunological significance compared to TECs. Recently, new technologies have made it possible to identify and characterize mesenchymal stromal cells in the thymus, revealing their unique functions in thymic organogenesis and T cell development. This review outlines the current views on mesenchymal stromal cells in the thymus, particularly highlighting the newly discovered function of thymic fibroblasts in T cell repertoire selection.
High-throughput methods to identify male Cannabis sativa using various genotyping methods
Authors: Torres Anthony , Pauli Christopher , Givens Robert , Argyris Jason , Allen Keith , Monfort Amparo , Gaudino Reginald J. 
Published: November 2022

Abstract
Background
Cannabis sativa is a primarily dioecious angiosperm that exhibits sexual developmental plasticity. Developmental genes for staminate male flowers have yet to be elucidated; however, there are regions of male-associated DNA from Cannabis (MADC) that correlate with the formation of pollen producing staminate flowers. MADC2 is an example of a PCR-based genetic marker that has been shown to produce a 390-bp amplicon that correlates with the expression of male phenotypes. We demonstrate applications of a cost-effective high-throughput male genotyping assay and other genotyping applications of male identification in Cannabis sativa.

Methods
In this study, we assessed data from 8200 leaf samples analyzed for real-time quantitative polymerase chain reaction (qPCR) detection of MADC2 in a commercial testing application offered through Steep Hill Laboratories. Through validation, collaborative research projects, and follow-up retest analysis, we observed a > 98.5% accuracy of detection of MADC2 by qPCR. We also carried out assay development for high-resolution melting analysis (HRM), loop-mediated isothermal amplification (LAMP), and TwistDx recombinase amplification (RPA) assays using MADC2 for male identification.

Results
We demonstrate a robust high-throughput duplex TaqMan qPCR assay for identification of male-specific genomic signatures using a novel MADC2 qPCR probe. The qPCR cycle quotient (Cq) value representative of MADC2 detection in 3156 males and the detection of tissue control cannabinoid synthesis for 8200 samples and the absence of MADC2 detection in 5047 non-males demonstrate a robust high-throughput real-time genotyping assay for Cannabis. Furthermore, we also demonstrated the viability of using nearby regions to MADC2 with novel primers as alternative assays. Finally, we also show proof of concept of several additional commercially viable sex determination methodologies for Cannabis sativa.

Discussion
In industrial applications, males are desirable for their more rapid growth and higher quality fiber quality, as well as their ability to pollinate female plants and produce grain. In medicinal applications, female cultivars are more desirable for their ability to produce large amounts of secondary metabolites, specifically the cannabinoids, terpenes, and flavonoids that have various medicinal and recreational properties. In previous studies, traditional PCR and non-high-throughput methods have been reported for the detection of male cannabis, and in our study, we present multiple methodologies that can be carried out in high-throughput commercial cannabis testing.

Conclusion
With these markers developed for high-throughput testing assays, the Cannabis industry will be able to easily screen and select for the desired sex of a given cultivar depending on the application.
Experimental study of bluntness effects on hypersonic boundary-layer transition over a slender cone using surface mounted pressure sensors
Authors: Huang Ranran , Cheng Jiangyi , Chen Jianqiang , Yuan Xianxu , Wu Jie 
Published: November 2022

AbstractIn this work, we studied the bluntness effect on the hypersonic boundary-layer transition over a slender cone at Mach 6 with interchangeable tips in a noisy Ludwieg tube tunnel before the so-called “transition reversal” phenomenon occurs. The evolution of instability waves is characterized using surface flush-mounted pressure sensors deployed along the streamwise direction within unit Reynolds number from 4E+ 6/m ≤ Reunit ≤ 10E+ 6/m, and the bluntness of the cone nose ranges from 0.1 mm to 5 mm. Power spectral density (PSD) of pressure fluctuation indicates that small nose bluntness (ReR ≤ 2000) has little influence on the evolution of instability waves along the hypersonic boundary-layer, whereas with a moderate nose size (2000 ≤ ReR ≤ 5000), the hypersonic boundary layer transition is delayed monotonically as the nose radius increases before the boundary-layer turns into fully laminar without instability waves. The delaying effect can be attributed to the increased entropy-layer swallowing distance with a large tip radius. Instability wave characterization reveals that the second mode instability wave plays a dominant role before the transition reversal happens. The quadratic phase locking of second mode instabilities can be identified by bispectral analysis, and it attenuates as the nose tip radius increases.
Genetic variation in toll-like receptor 4 gene with primary antiphospholipid syndrome susceptibility: a cohort of Egyptian patients
Authors: Mahdy Sara H. , Abd Elkader Nour M. , Kassim Nevine A. , ElHady Mostafa M. 
Published: November 2022

Abstract
Background
As toll-like receptor 4 (TLR4) plays important roles in cellular immunity and TLR4 polymorphisms have been shown to be associated with susceptibility to a range of diseases, the present study aimed to investigate the association between TLR4 gene polymorphisms and the incidence of primary antiphospholipid syndrome (PAPS).

Methods
Two TLR4 single nucleotide polymorphisms (rs4986790 and rs4986791) were assessed in 110 subjects of Egyptian ethnicity, including 65 female patients with PAPS and 45 matched healthy controls, using polymerase chain reaction-restriction fragment length polymorphism. Results were verified using automated sequencing.

Results
The homozygous wild-type (AA, aspartic acid) rs4986790 variant and (CC, threonine) rs4986791 variant were the predominant genotypes in the control and PAPS groups.

Conclusion
The results of this preliminary study of TLR4 gene variants among patients with PAPS in an Egyptian population found no association between the rs4986790 and rs4986791 variants and susceptibility to PAPS.
Cognitive impairment in different hemodialysis techniques
Authors: Abdelwahab Mahmoud Mohamed Abdelsalam , Sabri Youmna , Elkalla Ibrahim Hamdey Rashed , Nassar Mohammed Kamal , Abdel-Gawad Sara M. , Gomaa Zinab Abo Elfotouh 
Published: November 2022

Abstract
Background
Cognitive impairment is one of the most common, often untreated, comorbidities affecting patients with chronic kidney disease treated with hemodialysis. Increased mortality, poor compliance, depression, and poor quality of life were all linked to cognitive impairment in the hemodialysis population. The intradialytic exercise proved to be beneficial in improving patients’ quality of life, among other positive effects. Cooling dialysate has various positive effects, including improved patients’ mood and decreased hypotensive episodes during hemodialysis. The study aims to assess the effect of intradialytic exercise and cool hemodialysis on cognitive function in patients on hemodialysis.

Results
The study showed no significant effect of both interventions on the patient’s cognitive functions. However, cooling dialysate showed an improvement in the severity of depressive symptoms by Beck’s Depression Inventory II (p = 0.02). On the other hand, Mini-International Neuropsychiatric Interview showed an increase of patients diagnosed with depression in the intradialytic exercise group.

Conclusions
Both interventions had no significant effect on the mean scores of the Montreal Cognitive Assessment, which are lower in our sample than in the general population (23.9–25). Potential implications include the importance of managing psychiatric comorbidities in patients on hemodialysis. Cooling dialysate can be adopted to alleviate depressive symptoms in patients on hemodialysis.
The role of dynamic contrast-enhanced CT in characterization of solitary solid pulmonary nodules
Authors: EL-Metwally Dina , EL-Bakry Magda Ali Hany , EL-Adalany Mohamed Ali , Adel Shaimaa , Mohsen Mohamed 
Published: November 2022

Abstract
Background
Incidental indeterminate solitary solid pulmonary nodule is a progressively common finding on CT worldwide. Once detected, there are a number of imaging modalities that can be done to help in nodule characterization and differentiating benign from malignant nodules. Through these imaging modalities, there are PET CT, SPECT and dynamic CE-CT. Dynamic CE-CT is a functional test that help in assessment of the vascularity of the nodule which reverb the degree of angiogenesis of that nodule so can help in differentiating benign from malignant pulmonary nodules. The purpose of this study was to evaluate the role of Dynamic CE-CT in characterization of solitary pulmonary nodules. Detect what are the important parameters on dynamic CE-CT to differentiate benign from malignant nodules and detect their cutoff values.

Results
The pre-enhancement value shows cutoff point of 26.50 HU with sensitivity 93.8% and specificity 75% with accuracy rate 90% in differentiating benign from malignant pulmonary nodules. Peak enhancement value (at 2 min) shows cutoff point of 40.00 HU with sensitivity 96.9% and specificity 87.5% with accuracy rate 95% in differentiating benign from malignant pulmonary nodules. Net enhancement value shows cutoff point of 19.00 HU with sensitivity 96.9% and specificity 87.5% with accuracy rate 95% in differentiating benign from malignant pulmonary nodules.

Conclusion
Dynamic CE-CT is a useful tool in differentiating benign from malignant pulmonary nodules. Peak and net enhancement values are important parameters with high sensitivity and specificity in differentiating benign from malignant pulmonary nodules.
A conceptual framework for understanding the intrinsic contestation of cultural heritage tourism in Chinese qiaoxiang
Authors: Jiang Ting 
Published: November 2022

AbstractCultural heritage in Chineseqiaoxiangis constructed where Chinese and foreign cultures gather, connecting overseas Chinese with their country of origin. However, conflicts concerning this type of heritage comes out frequently, such as house reconstruction clash, host-guest conflicts and destructive competition in heritage tourism. The economic and cultural duality of heritage is perceived as the source of intrinsic contestation in heritage tourism, and conflicts related to different types of heritage may take on different appearances and causes. Extant tourism studies have generalised cultural heritage in Chineseqiaoxiang, neglecting the unique characteristics of the diaspora and their corresponding influence on heritage protection and utilisation, that makes the reasons for these conflicts remain unclear. By answering the basic question of what heritage is, this research proposes an analytical framework to understand the intrinsic contestation of cultural heritage tourism in Chineseqiaoxiang. The paper points out that the contradiction between ‘ancestral root culture’ and modernity and between flow and stillness is the trigger for intrinsic contestation. The paper also summarises the core issues in conflicts that need further discussion by answering the questions of ‘what is heritage’, ‘whose heritage’ and ‘how to interpret heritage’. The core issues include heritage selection and identification, the commercialisation of ‘home’, heritage interpretation and so on.