bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surveillance of Enterobacter cloacae complex colonization and comparative analysis of different typing methods on a neonatal intensive care unit in Germany
Authors: Wendel Andreas F. , Peter Daniel , Mattner Frauke , Weiss Michael , Hoppenz Marc , Wolf Sophia , Bader Baris , Peter Silke , Liese Jan 
Published: April 2022

Abstract
Background
Enterobacter cloacae complex is a group of common opportunistic pathogens on neonatal intensive care units. Active microbiological screening to guide empirical antimicrobial treatment or to detect transmission events is recommended in high-risk preterm neonates. A rise in colonization with E. cloacae complex was observed in a German perinatal centre. The aim of this study was to evaluate the performance of different typing techniques using whole genome sequencing (WGS) as a reference.

Methods
Enterobacter cloacae complex isolates from clinical and screening specimens with an epidemiological link to the neonatal intensive care units were further assessed. Identification and antibiotic susceptibility testing was performed by a combination of VITEK2 (bioMérieux) and MALDI-TOF (Bruker Daltonics), followed by RAPD/rep-PCR and PFGE (XbaI). Retrospectively, all isolates were analyzed by Fourier-transform infrared (FTIR) spectroscopy (IR Biotyper, Bruker Daltonics). Whole genome sequencing with SNP-based clustering was used as the reference method. Furthermore, resistome analysis, sequence type and species identification were derived from the WGS data. Transmission analysis was based on epidemiological and typing data.

Results
Between September 2017 and March 2018 32 mostly preterm neonates were found to be colonized with E. cloacae complex and 32 isolates from 24 patients were available for further typing. RAPD/rep-PCR and PFGE showed good concordance with WGS whereas FTIR displayed mediocre results [adjusted rand index (ARI) = 0.436]. A polyclonal increase and two dominant and overlapping clonal clusters of two different E. hormaechei subspecies were detected. Overall, four different species were identified. Genotyping confirmed third-generation cephalosporin resistance development in isolates of the same patient. During the six-month period several infection prevention interventions were performed and no E. cloacae complex isolates were observed during the following months.

Conclusions
Interpretation of the microbiological results alone to detect transmission events is often challenging and bacterial typing is of utmost importance to implement targeted infection control measures in an epidemic occurrence of E. cloacae complex. WGS is the most discriminatory method. However, traditional methods such as PFGE or RAPD/rep-PCR can provide reliable and quicker results in many settings. Furthermore, research is needed to quickly identify E. cloacae complex to the species level in the microbiological laboratory.
18F-FDG-PET/CT can be used to predict distant metastasis in hypopharyngeal squamous cell carcinoma
Authors: Suzuki Shinsuke , Toyoma Satoshi , Abe Tomoe , Endo Tentaro , Kouga Teppei , Kaswasaki Yohei , Yamada Takechiyo 
Published: April 2022

Background Hypopharyngeal squamous cell carcinoma (HPSCC) has a high rate of distant metastasis, resulting in poor prognosis. The role of the maximum standardized uptake value (SUVmax), which was assessed via pretreatment 18-fluorodeoxyglucose positron emission tomography (FDG-PET), and computed tomography (CT) was examined, for predicting distant metastasis and survival. Methods This study included 121 patients who underwent pretreatment FDG-PET/CT scanning and subsequent treatment for HPSCC. The SUVmax was measured via FDG-PET/CT. A receiver operating characteristic (ROC) curve analysis was used to determine whether the SUVmax was a predictor of distant metastasis and to select the best cutoff value. Univariate and multivariate Cox hazard regression analyses were used in identifying associations between the SUVmax and other clinicopathological factors with distant metastasis-free survival. Results Distant metastases were identified in 33 patients during the median follow-up of 24 months after treatment. The ROC curve analysis determined that SUVmax was predictive of distant metastasis and identified a SUVmax of 13.9 as the best potential cutoff value. The univariate analysis showed that T and N classification, clinical stage, and SUVmax were significantly related to distant metastasis. However, in multivariate analysis, an SUVmax ≥ 13.9 was the only independent predictor of distant metastasis. Patients with high SUVmax values displayed significantly shorter distant metastasis-free survival and overall survival. Conclusions SUVmax determined via pretreatment FDG-PET/CT is useful for predicting distant metastasis, distant metastasis-free survival, and overall survival in patients with HPSCC. Graphical Abstract
Redistribution of critical drugs in shortage during the first wave of COVID-19 in France: from operating theaters to intensive care units
Authors: Chapuis Claire , Collomp Rémy , Albaladejo Laura , Terrisse Hugo , Honoré Stéphane , Bosson Jean-Luc , Bedouch Pierrick , Albaladejo Pierre 
Published: April 2022

Abstract
Background
Tension in the supply of highly consumed drugs for patients with COVID-19 (propofol, midazolam, curares) led the French government to set up a centralized supply of hospitals with distribution based on the number of resuscitation beds in March 2020. The French Societies of Clinical Pharmacy and of Anesthesia and Critical Care aimed to evaluate the changes in total needs and the distribution between anesthesia and critical care activities (CCU), to prepare resumed surgical activity.

Methods
National declarative survey among pharmacists, via an online form (SurveyMonkey®), was conducted in April and May 2020. The analysis focused on quantities dispensed during the whole year 2019, and March and April of year 2019 and 2020 for the drugs subject to quota, and on their distribution in CCU and operating theaters.

Results
For the 358 establishments (47% public, 53% private), dispensations in CCU in March 2020 compared to March 2019 increased, respectively: propofol (+81%), midazolam (+125%), cisatracurium (+311%), atracurium (+138%), rocuronium (+119%); and decreased for anaesthesia: propofol (−27%), midazolam (-10%), cisatracurium (−19%), atracurium (−27%), rocuronium (+16%).

Conclusions
Variation of dispensations between CCU and others was directly related to the increase of COVID patients in CCU and the decrease in surgical activity. Each establishment could receive up to five or six different presentations and concentrations, leading to a major risk of medication error. This collaborative national survey provided accurate data on the drugs’ usual consumption. This work emphasized the need for a strong collaboration between pharmacists and anesthesiologists and intensive care physicians. It was further used by the Health Ministry to adjust the drug distribution.
Extending mixed embeddedness to a multi-dimensional concept of transnational entrepreneurship
Authors: Yamamura Sakura , Lassalle Paul 
Published: April 2022

AbstractMigrant entrepreneurship in times of transnational migration go beyond locally serving markets and increasingly operate transnationally. The mixed embeddedness by Kloosterman and Rath has become the main concept to analyze such migrants’ entrepreneurship as it accounts for the multiple embeddedness of entrepreneurs in the variety of social and institutional contexts at multiple levels. This concept, however, does not yet accommodate the transnational dimensions of migrant entrepreneurship, which is still rather nascent in entrepreneurship research. Transnationalism is multi-dimensional in its nature as pointed out by migration researchers as Vertovec, and transnational migrants’ embeddedness appears to go beyond the notion of being simply dually embedded in two locations but rather should be conceived as being in one larger transnational field—though such aspects are not conceptually accounted in the mixed embeddedness approach. Taking this as a starting point, we propose to analyze the conditions that allow migrant entrepreneurs to engage in transnational activities. Our proposed framework bases on empirical research with 36 Polish entrepreneurs in the EU labor market, by drawing their opportunities from different levels and contexts of transnationalism. Following the research question on which levels and dimensions of embeddedness in the transnational field contribute to transnational entrepreneurship, we develop a novel refined framework of mixed embeddedness to analyze transnational entrepreneurship. It clarifies the entrepreneurial context by analytically and systematically subdividing components across dimensions (political, social and economic), and rearranging institutional elements and structures in each dimension according to respective levels (macro, meso, and micro). Emphasizing the role of conditions at the meso-level, the novel analytical framework better incorporates the multi-dimensionality and multi-levelling of transnational entrepreneurial activities of migrants. This model can be used as a tool for future comparative analyses of migrant entrepreneurship in different transnational contexts, it also contributes to the concretization of the transnational nature of transnational migrant entrepreneurship.
A novel simple traumatic brain injury mouse model
Authors: Chen Chen , Hou Jiawei , Lu Junfeng , Zhu Zeyu , Yang Yang , Peng Weijia , Pi Rongbiao 
Published: April 2022

Abstract
Background
Traumatic brain injury, one of the leading causes of death in adults under 40 years of age in the world, is frequently caused by mechanical shock, resulting in diffuse neuronal damage and long-term cognitive dysfunction. Many existing TBI animal models revival with expensive equipment or special room are needed or the processes of operations are complex and not easy to be widely used. Therefore, a simpler TBI model needs to be designed.

Methods
Our TBI model is an innovation of the modeling method through air guns shutting rubber bullets. A core facet is the application of our designed rubber bullet impact device. It could focus the hitting power to the fixed site of the brain, thus triggering a mild closed head injury. Moreover, the degree of damage can be adjusted by the times of shots.

Results
Our model induced blood-brain barrier leakage and diffused neuronal damage. Besides, it led to an increased level of Tau phosphorylation and resulted in cognitive dysfunction within several weeks post-injury.

Conclusion
Our TBI model is not only simple and time-saving but also can simulate mild brain injuries in clinical. It is suitable for exploring pathobiological mechanisms as well as a screening of potential therapies for TBI.
Advances in targeting central sensitization and brain plasticity in chronic pain
Authors: Bazzari Amjad H. , Bazzari Firas H. 
Published: April 2022

AbstractMaladaptation in sensory neural plasticity of nociceptive pathways is associated with various types of chronic pain through central sensitization and remodeling of brain connectivity. Within this context, extensive research has been conducted to evaluate the mechanisms and efficacy of certain non-pharmacological pain treatment modalities. These include neurostimulation, virtual reality, cognitive therapy and rehabilitation. Here, we summarize the involved mechanisms and review novel findings in relation to nociceptive desensitization and modulation of plasticity for the management of intractable chronic pain and prevention of acute-to-chronic pain transition.
Safety and efficacy of posterior vertebral column resection in complex pediatric deformities
Authors: Sorour Islam , Elhabashy Abdelrahman Magdy , Fayed Ahmed Abdelaziz 
Published: April 2022

Abstract
Background
Vertebral column resection (VCR) is a well-known technique used for correction of complex spinal deformities. VCR could be done through a posterior only approach (Pvcr), or a combined anteroposterior approach, with almost comparable results. Early studies of Pvcr have reported high rates of complications, while subsequent studies have reported a reasonable complication rate. In this study, the authors represent and evaluate the initial results of using the Pvcr technique to correct complex pediatric deformities.

Objective
To evaluate the safety and efficacy of performing Pvcr to correct complex pediatric deformities.

Methods
Retrospective cohort study of data was collected from the database of pediatric deformity patients who were operated for correction of their deformities using posterior instrumentation and Pvcr at a single institution from 2015 to 2019.

Results
Twenty-one pediatric patients with a mean age 15.2 ± 3.5 years were enrolled in this study. The mean follow-up period was 26.3 ± 3.1 months. The mean Cobb angle has been decreased significantly from 82.9 ± 23.9 degrees to 28.8 ± 14.2 immediately after correction (correction rate 66.9 ± 10.8%, p < 0.001) with slight increase to 30.2 ± 14.9 after 24 months of follow-up (correction loss 4.3 ± 3.1%). The mean kyphotic angle has decreased significantly from 74.1 ± 15.9 to 25.4 ± 4.5 immediately after correction (correction rate 65.4 ± 2.9%, p < 0.001) with slight increase to 26.7 ± 5.2 after 24 months of follow-up (correction loss 4.8 ± 3.5%). The mean estimated blood loss was 2816.7 ± 1441.5 ml. The mean operative time was 339 ± 84.3 min. Self-image domain (part of SRS-22 questionnaire) has significantly improved from a mean preoperative of 2.3 ± 0.5 to a mean postoperative of 3.9 ± 0.4 after 24 months of correction (p < 0.001). As regards complications, chest tubes were inserted in 17 cases (81%), one case (4.8%) had suffered from deep wound infection and temporary respiratory failure, while 3 cases (14.3%) had neurological deficits.

Conclusion
Posterior vertebral column resection is considered a highly effective release procedure that aids in the correction of almost any type of complex pediatric deformities with a correction rate reaching 66.9 ± 10.8%. However, Pvcr is a challenging procedure with high estimated blood loss and risk of neurological deficits, so it must be done only by experienced spine surgeons in the presence of good anesthesia and neuromonitoring teams.
Fourier transform infrared spectral features of plant biomass components during cotton organ development and their biological implications
Authors: HE Zhongqi , LIU Yongliang , KIM Hee Jin , TEWOLDE Haile , ZHANG Hailin 
Published: April 2022

AbstractBackgroundThe majority of attenuated total reflection Fourier transform infrared (ATR FT-IR) investigations of cotton are focused on the fiber tissue for biological mechanisms and understanding of fiber development and maturity, but rarely on other cotton biomass components. This work examined in detail the ATR FT-IR spectral features of various cotton tissues/organs at reproductive and maturation stages, analyzed and discussed their biological implications.ResultsThe ATR FT-IR spectra of these tissues/organs were analyzed and compared with the focus on the lower wavenumber fingerprinting range. Six outstanding FT-IR bands at 1 730, 1 620, 1 525, 1 235, 1 050 and 895 cm−1represented the major C=O stretching, protein Amide I, Amide II, the O–H/N–H deformation, the total C–O–C stretching and the β-glycosidic linkage in celluloses, respectively, and impacted differently between these organs with the two growth stages. Furthermore, the band intensity at 1 620, 1 525, 1 235, and 1 050 cm−1were exclusively and significantly correlated to the levels of protein (Amide I bond), protein (Amide II bond), cellulose, and hemicellulose, respectively, whereas the band at 1 730 cm−1was negatively correlated with ash content.ConclusionsThe resulting observations indicated the capability of ATR FT-IR spectroscopy for monitoring changes, transportation, and accumulation of the major chemical components in these tissues over the cotton growth period. In other words, this spectral technology could be an effective tool for physiological, biochemical, and morphological research related to cotton biology and development.
Sudden cardiac death in heart failure with preserved ejection fraction: an updated review
Authors: Wu Shang-Ju , Hsieh Yu-Cheng 
Published: April 2022

AbstractDespite the advances in medical and device therapies for heart failure (HF), sudden cardiac death (SCD) remains a tremendous global burden in patients with HF. Among the risk factors for SCD, HF has the greatest impact. Previous studies focusing on patients with systolic dysfunction have found several predictive factors associated with SCD, leading to the subsequent development of strategies of primary prevention, like placement of implantable cardioverter-defibrillator (ICD) in high-risk patients. Although patients with HF with preserved ejection fraction (HFpEF) were less prone to SCD compared to patients with HF with reduced ejection fraction (HFrEF), patients with HFpEF did account for a significant proportion of all HF patients who encountered SCD. The cutoff value of left ventricular ejection fraction (LVEF) to define the subset of HF did not reach consensus until 2016 when the European Society of Cardiology proposed a new classification system by LVEF. There is a great unmet need in the field of SCD in HFpEF regarding risk stratification and appropriate device therapy with ICD implantation. In this article, we will approach SCD in HFpEF from HFrEF subsets. We also aim at clarifying the mechanisms, risk factors, and prevention of SCD in HFpEF.
Microwave pyrolysis of sludge: a review
Authors: Li Shaobai , Li Caixia , Shao Zhiguo 
Published: April 2022

AbstractAs an environmentally friendly energy recovery technology, microwave pyrolysis has huge development potential in sludge resource treatment. This paper comprehensively reviews the progress of microwave pyrolysis of sludge, focusing on the mechanisms and development status of microwave pyrolysis equipment. The effects of pyrolysis temperature, heating rate, microwave absorbers, sludge properties and catalysts on microwave pyrolysis efficiency and its products are also discussed. Finally, the differentiation compared with conventional pyrolysis is summarized. It is suggested that target products can be controlled directionally by changing the pyrolysis conditions and exploring the harmful products produced in the microwave pyrolysis process. Future research directions are proposed to help the subsequent extensive application of microwave pyrolysis technology in sludge treatment.
The challenges of the management of the incisional hernia in children and infants: a retrospective experience of a tertiary hospital
Authors: Khirallah Mohammad Gharieb , Elkhadrawy Osama Helmy , Eldessouki Nagi Ebrahim , Elgendy Ahmed 
Published: April 2022

Abstract
Background
Incisional hernia in children and infants represents a major complication following exploration. The condition is associated with prolonged hospital stay, readmission, and the need for another operation to treat the resulting incisional hernia. The incidence is variable worldwide. The leading causes are still indeterminate as well as the management strategy. We aimed at studying incisional hernia in children and infants at a tertiary level hospital as regards presenting symptoms, methods of management, and its impact on the quality of life of patients.

Results
A retrospective study included all infants and children presented with incisional hernia. Patients were categorized in to two groups according to the age of presentation, group A patients younger than 2 years of age and group B patients older than 2 years. All data related to the first operation in addition to data related to repair of the incisional hernia were collected in special charts. The study included 67 patients. the median age in group A was 6.5 months vs 10.5 years in group B. Urgent presentation was found in 35.4% of cases in group A vs 19.44% of cases in group B. Tissue repair was used as the definitive management in 64.5% of cases in group A and in 52.7% of cases in group B.

Conclusion
Incisional hernia in infants and children is a major complication. The management is associated with prolonged hospital stay, financial burden, and deep impact on the quality of life. It is to some extent difficult to predict its onset.
Use of acetylsalicylic acid and agronomic performance of potatoes in Lima region
Authors: Contreras-Liza Sergio , Vargas-Luna Luis 
Published: April 2022

Abstract
Background
Salicylic acid participates in the regulation of the plant's response to a series of environmental stresses such as extreme temperatures, salinity, and oxidative condition of potato growth, so it is necessary to determine a safe application dosage for potato in field conditions.

Methods
The purpose of the research was to evaluate the effect of increasing doses of acetylsalicylic acid on the agronomic traits of two potato cultivars in Lima, Peru. In a field experiment, an RCBD with 3 replications was used under a split-plot arrangement in which five doses (0.0, 0.2, 0.4, 0.6, and 0.8 mM) of acetylsalicylic acid plus control with Biol (biostimulant) were assigned to plots, and two potato cultivars ('Perricholi' and 'Unica') were assigned to subplots. The average temperatures were between 15 and 21º C, the air relative humidity was between 61 and 73% and the soil was loam textural class, free of salts.

Results
No statistical differences were found for the number and weight of tubers and biomass due to the effect of the application of the treatments. The effects of doses of acetylsalicylic acid showed statistical differences for sprouting, vegetative vigor, and percentage of flowering; likewise, significant interactions (p < 0.05) were shown between potato cultivars and dose of acetylsalicylic acid for the number of stems per plant and percentage of flowering, which indicated a specific effect for the dose of acetylsalicylic acid in each potato cultivar. Statistical differences (p < 0.05) were found between the two potato cultivars for most of the characters studied.

Conclusion
No dose of the product significantly affected the potato yield in two potato cultivars however, the effect of the dosage of acetylsalicylic acid improved the performance of the crop in terms of sprouting in the field, vegetative vigor, a greater number of stems per plant as well as stimulation of flowering, with respect to control.
Suicidal vulnerability in older adults and the elderly: study based on risk variables
Authors: Sánchez-Teruel David , Robles-Bello María Auxiliadora , Sarhani-Robles Aziz 
Published: April 2022

BackgroundPredicting suicidal vulnerability based on previous risk factors remains a challenge for mental health professionals, especially in specific subpopulations.AimsThis study aimed to use structural equation modelling to assess which sociodemographic and clinical variables are most predictive and modulating of repeated self-injury or reattempts at suicide in older adults and the elderly with previous attempts.MethodWe obtained digital data for 619 people (N= 342; 55.3% women), aged 50–96 years (mean 71.2 years, s.d. 3.65), who presented to the emergency department with a repeated self-injury or suicide attempt. Data were collected from several public and private hospitals in southern Spain.ResultsThere were different sociodemographic and clinical profiles between people who repeat self-injury and those who reattempt suicide. In addition, we show that outcome variables may directly or indirectly modulate these behaviours.ConclusionsThe study findings provide only a limited insight into suicidal vulnerability in older people, and there is an urgent need for specific care protocols for the prevention of repeated self-injury or reattempts at suicide that are adapted to the psychosocial characteristics of this age group. There is also a need to improve social and health alert actions for older adults and the elderly who present with suicide risk profiles, and the presence of mental health professionals in hospital emergency departments should be improved.