bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNAs 7/17/155 As A Potential Breast Tumor Stemness miRNA Cluster: Detection of Breast Cancer Grade Specific miRNA-mRNA Interaction Network Inspiration from Mammary Gland Development
Authors: Khodayari Saeed , Khodayari Hamid , Jallali Haniyeh , Saeedi Elnaz , Faryabi Ali , Yeganeh Meghdad , Mohammadnejad Ahad , Razavi Amirnader Emami , Dashtkoohi Mohammad , Hadjilooei Farimah , Shirkoohi Reza , Omranipour Ramesh , Nayernia Karim , Mahmoodzadeh Habibollah 
Published: October 2022

AbstractBackground The process of breast tumor dedifferentiation is complex and unclear. The mechanism represents the origin of the genesis and development of high-grade breast stem cells. It seems that microRNAs have crucial regulatory functions in this complicated phenomenon. The main objective of this study is to identify a potential "breast tumor stemness miRNA cluster" using an in silico strategy and qRT-PCR validation guided by the molecular pattern of mammary gland development (MGD). Methods Microarray databases GEO and ArrayExpress were used to determine mRNA and microRNA expression in different grades of breast carcinoma (BC). Differential gene expression of mRNA (GSE29044) and miRNA (GSE4566) in three grades of BC was analyzed using GEO2R compared with normal tissue. The enrichment results revealed MGD -associated mechanisms and target mRNAs. Using the BC database, the interaction between target mRNAs and significantly altered miRNAs (PV ≤ 0.05) in each BC grade was found by miRNet. After confirming our results using the GSE26659 data, the expression of the target miRNAs in tissue samples (24 BC, 17 normal tissues) was examined by real-time PCR. miRwalk and the STRING database discovered the miRNAs of interest and mRNA networks. Results The MGD stages of puberty, pregnancy and lactation, and mammary gland epithelial development were significantly involved in the upregulated genes of GI and GII tumors. No significant upregulated MGD mechanisms were detected in GIII BC. In silico analysis revealed that miRs 7/17/155 had an upregulation pattern and miR-26a had a downregulation pattern. qPCR showed that the miRNAs 7/17/155 were significantly upregulated in GIII tumors (PV ≤ 0.05), while there were no notable changes in miR-26a. EGFR was the central node regulated by the miR 7/17/155 intermediate mRNA cluster. Conclusions Our results suggest that microRNAs 7/17/155 may be a potential cluster associated with formation of breast tumor stemness. This cluster can be used for the breast cancer dedifferentiation therapy or molecular classification of mammary tumor differentiation status.
Integrating External Factors and Technology Acceptance Model to Understand Scholar Intention and Use of Recommendation System for Course Selection.
Authors: Zameer Zameer Gulzar , Fathima Fatima Amer Jid Almahri , Afrah Afrah Fathima 
Published: October 2022

Abstract
There has been a huge increase in e-learning courses, and the Recommendation systems (RSs) are useful tools for narrowing down the course options and expose students to the courses that suit their needs. The majority of the research related to recommendation systems focus on effectiveness rather than factors influencing its acceptability, and in practice, user satisfaction cannot be explained by accuracy alone. This study will consider course recommendation system and investigate the acceptance of the courses suggested by the recommender system (RS) to scholar’s or research students based on their learning needs which in turn will help to find out why some individuals accept new technology while others resist. Therefore, research scholars (n = 150) who willingly engaged in this study were asked to use the RS and complete a questionnaire also based on their experience as part of a self-administrated longitudinal survey. This study assessed external factors such as perceived availability, relevance, and experience, which were not explained by the Technology Acceptance Model (TAM). The results confirm that the extended TAM provides a valuable theoretical model, which helps to understand scholar’s acceptance of RS, and other factors positively that affect the original variables of TAM. Hence, a new modified TAM that incorporates three external factors has been offered. The considerable value calculated for Cronbach’s alpha confirms that the results are valid and reliable. The observed results will help recommendation system developers to maximize user experience because the implications of this research effort are critical for instructors, scholar’s, and institutions as well.
Variation in the local grey mullet populations (Mugil cephalus) on the western Pacific fringe
Authors: Kuo Chien-Hsien , Lee Sin-Che , Du Shin-Yi , Huang Chao-Shen , Lin Hung-Du 
Published: October 2022

AbstractUnderstanding population genetic structures is crucial for planning and implementing conservation programmes to preserve species’ adaptive and evolutionary potential and thus ensure their long-term persistence. The grey mullet (Mugil cephalus) isa globally distributed coastal fish. Its populations in waters surrounding Taiwan on the western Pacific fringe are divided into at least two stocks (migratory and residential), but questions remain regarding their genetic divergence and gene flow. To cast more light on this, allozyme variations at 21 presumptive gene loci of 1,217 adult grey mullets from 15 localities in Japan, Taiwan and mainland China, and four gene loci from 1,470 juveniles from three localities in Taiwan were used to investigate patterns of genetic variation. The mean expected heterozygosity (He) was 0.128—ranging from 0.031 (Matsu) to 0.442 (Kaoping)—and the mean observed heterozygosity (Ho) was 0.086—ranging from 0.017 (Kaohsiung) to 0.215 (Kaoping). Both AMOVA and the high overall meanFSTof 0.252 indicated enormous genetic differentiation among populations and the positive mean value of FISwas 0.328, indicating a deficiency of heterozygotes. PCA analysis indicated that the samples ofM. cephaluscould be split into four groups and STRUCTURE analysis showed that all individuals were grouped into three genetic clusters. Results of mutation-drift equilibrium tests did not suggest that the populations experienced any recent genetic bottleneck. Results from all localities in the present investigation showed that significant change in the GPI-A genotype frequencies with latitudes—e.g., an increase inGPI-A * 135/135homozygote frequencies andGPI- A * 100/100frequencies were highly correlated with latitudinal cline. T All migratory populations with the GPI-A genotype were almost exclusively theGPI-A * 100/100homozygote. During the life history ofM. cephalus, theGPI-A * 100/135heterozygote frequency significantly decreases with age. Based on these data, we suggested that each GPI-A genotype represents trait combinations of higher fitness in some portions of the environment. Furthermore, the genotypic frequencies change in accordance with life stages, suggesting that selection occurs throughout the life span.
Predictors of cancer rehabilitation medicine referral and utilization among cancer survivors
Authors: Brick Rachelle , Natori Akina , Moreno Patricia , Molinares Diana , Koru-Sengul Tulay , Penedo Frank 
Published: October 2022

Abstract
Purpose
Cancer survivors experience high rates of physical inactivity that often go unaddressed. The My Wellness Check program (MWC) is an EHR-integrated screening and referral system that includes surveillance of physical activity and triage to cancer rehabilitation medicine services. This study examined assessment of physical activity and subsequent referrals to cancer rehabilitation medicine.
Methods
A secondary analysis was performed for survivors who completed the MWC between April 2021 and January 2022. Univariable and multivariable logistic regression modeled determinants of qualification for a physical activity referral and provider completion of referral to cancer rehabilitation medicine. Referral was based on the Moving through Cancer questionnaire. Adjusted odds ratios (aOR) and corresponding 95% confidence intervals (95% CI) were calculated.
Results
There were 1,174 survivors who completed the physical activity survey, of which 46% (n = 540) qualified for a physical activity referral. After controlling for group differences, qualification for a referral included moderate-severe physical dysfunction (aOR: 1.815; 95% CI: 1.177, 2.798), and Hispanic or Latino ethnicity (aOR: 0.744; CI: 0.574, 0.963). Only 31% (n = 168) received a completed physician referral to cancer rehabilitation medicine following screening. No patient-level factors were significantly associated with receiving a completed physician referral. Following referral, 8% (n = 13) utilized cancer rehabilitation medicine services.
Conclusions
Patient-level and clinical factors may predict qualification for physical activity referrals; however, they don’t appear to predict referral completion to cancer rehabilitation medicine services. Future research should focus on potential provider- and organization-level factors that interact and influence referral to and utilization of cancer rehabilitation medicine services.
Serum Klotho and Cognitive Functioning in Older Adults Aged 60 and 79: The National Health and Nutrition Examination Survey 2011 to 2014
Authors: Ge Song , Dong Fanghong , Xu Li , Tian Chong , Yang Chih-Hsiang , Wei Jingkai 
Published: October 2022

Abstract
Objectives
Klotho is found to be associated with better cognitive outcomes in selected small sample of aged population. We aimed to examine the association of serum klotho with cognitive function among older adults using a nationally representative sample of the U.S.
Method
A total of 2,173 older adults aged 60–79 years in the National Health and Nutrition Examination Survey from 2011 to 2014 were included. Klotho was measured and quantified in laboratory. Cognitive function was measured using the Consortium to Establish a Registry for Alzheimer’s Disease Word Learning subtest [CERAD-WL] immediate and delayed memory, the Animal Fluency test [AFT], the Digit Symbol Substitution Test [DSST]). Test-specific and global cognition z-scores were calculated based on means and standard deviations. Multivariable linear regression models were applied to examine the association of quartiles of serum klotho with test-specific and global cognition z-scores. Subgroup analysis was conducted by sex.
Results
Serum klotho level in the lowest quartile was associated with lower z-score for DSST (beta (β)=-0.13, 95% confidence interval (CI): -0.25, -0.01). For subgroup analysis, serum klotho level in the lowest quartile was associated with lower z-scores for DSST (β=-0.16, 95% CI: -0.32, -0.003) and global cognition (β=-0.14, 95% CI: -0.28, -0.01) among women. No significant association was found among men.
Conclusions
Low serum klotho was associated with poorer cognitive function, particularly among women. Future studies are expected to examine the longitudinal associations between klotho levels and cognitive outcomes.
Probabilistic Seismic Risk Assessment Framework: Case study Adapazari, Turkey
Authors: Sianko Ilya , Ozdemir Zuhal , Hajirasouliha Iman , Pilakoutas Kypros 
Published: October 2022

Abstract
While earthquakes can have a devastating impact on the economic growth and social welfare of earthquake prone regions, probabilistic seismic risk assessment can be employed to assess and mitigate such risks from future destructive events. In a previous study (Sianko, 2020), a probabilistic seismic hazard analysis (PSHA) tool based on the Monte-Carlo (MC) approach, was developed to predict the seismic hazard for high seismicity areas. In this study, a seismic risk assessment framework is developed incorporating the previously developed PSHA tool, with vulnerability functions based on various damage criteria, exposures and casualty models. Epistemic uncertainty is addressed using logic trees and distribution functions. The developed seismic risk assessment framework can estimate human and economic losses for particular return periods using an event-based stochastic procedure. The framework is applied to a case study area, the city of Adapazari in Turkey. Seismic risk assessment is carried out for different return periods to identify the most vulnerable areas of the city. The verification of the developed seismic risk framework is performed by comparing the predicted seismic losses to those observed during the 1999 Kocaeli earthquake that severely affected the city of Adapazari. The results of the study indicate that while overall predictions for extensive and complete damage states demonstrate strong correlation with the observed data, accurate risk predictions at the district level are not achievable without microzonation studies.
Perception and usage of short-term prednisone and prednisolone in dogs
Authors: Gober Margaret , Hillier Andrew 
Published: October 2022

Abstract
Background: Corticosteroids are widely used with a broad level of comfort in the hands of most veterinarians. With this level of comfort there may be complacency and underestimation of the impact the side effects of corticosteroids may have on a pet and pet owner.
Objective: The objective of this clinical study was to understand the experience and perceptions of an owner using anti-inflammatory doses of oral prednisolone and prednisone for up to 14 days. We hypothesized dogs receiving anti-inflammatory doses of prednisone and prednisolone would experience much greater rates of side effects by day 14 then reported in current literature.
Animals: There were 45 dogs initially enrolled in the study, with 33 dogs completing the study.
Results: At each study point, 31 owners provided results. On day 5, 74% (23/31) reported at least 1 change in their dog’s behavior including polyuria, polydipsia, polyphagia, polypnea and/or increased vocalization, with 11 individuals (35%) reporting these changes greatly increased. On day 14, 90% of owners (28/31) reported at least 1 change in their dog’s behavior including polyuria, polydipsia, polyphagia, and/or polypnea as the most common changes noted. Overall, 61% (19/31) of owners reported an increase in filling of the water bowl over baseline and one-third (11/31) of pet owners reported cleaning up urinary accidents for pets who had been continent prior to the start of the study. Pet owner steroid satisfaction remained high through day 14 at 4.5/5 (1= very unsatisfied, 5= very satisfied).
Conclusion: This study highlights the impact short term anti-inflammatory doses of prednisone or prednisolone have on dog behaviour and confirms the hypothesis that prednisone or prednisolone usage changes the behaviour of dogs included in this study as soon as day 5 post-treatment. By day 14, the majority of dogs experienced one or more behaviour changes, with polyuria and polydipsia most commonly reported. Adverse events were noted regardless of starting dosage or regimen. Although most pet owners expressed satisfaction with steroid treatment due to its high efficacy, 70% would select a more costly treatment if that treatment had fewer side effects.
The Effect of Empowerment Program to Reduce Sugar Consumption Based on the Multi-Theory Model on Body Mass Index and Abdominal Obesity in Iranian Women
Authors: Joveini Hamid , Sharifi Nader , Meymary Batool Kalate , Mehri Ali , Shahrabadi Reza , Rahmanian Vahid , Hashemian Masoumeh 
Published: October 2022

Abstract
Background Considering the prevalence of overweight and abdominal obesity in middle-aged women, this study was conducted to determine the effect of empowerment program to reduce sugar consumption based on the MTM on BMI and abdominal obesity in women aged 30-60 in Joven.
Methods This quasi-experimental study (include descriptive and interventional sections) was conducted on the Joven city in Khorasan Razavi province in northeastern Iran from October 2020 to August 2021. Sampling was performed as a multi-stage cluster. After a descriptive study, 128 women with the conditions to enter the intervention section of the study were selected. In the control group, 63 people and in the intervention group, 65 people were eligible to enter the study.
The educational intervention was performed in five 60-minute sessions for groups of 12 people. The instruments included the demographic questionnaire, sugar consumption checklist and researcher-made questionnaire based on MTM constructs. Before the intervention, one, three and six months after the intervention, the questionnaire was completed by both intervention and control groups also measurement of waist circumference and BMI were performed using standard instruments. The obtained data were analyzed by SPSS 17.
Results After the educational intervention, there was a significant difference between the intervention and control groups in all the MTM constructs. Also, six months after the educational intervention, BMI, waist circumference and amount of consumption of sugary substances decreased significantly in the intervention group (p<0.05).
Conclusion This study showed the effectiveness of designing the educational intervention based on the MTM in reducing the consumption of sugary substances in women aged 30-60.
Incidence and severity of immune-related hepatitis after dual checkpoint therapy is linked to younger age independent of herpes virus immunity
Authors: Zhang Zhen , Rafei-Shamsabadi David , Lehr Saskia , Buettner Nico , Diehl Rebecca , Huzly Daniela , Pinato David James , Thimme Robert , Meiss Frank , Bengsch Bertram 
Published: October 2022

Abstract
Background and Aims:
Dual immune checkpoint blockade (ICB) therapy can result in immune-related-adverse events (irAE) such as ICB-hepatitis. An expansion of effector-memory (TEM) CD4 T cells associated with antiviral immunity against herpesviridae was implicated in ICB-hepatitis. Notably, these memory subsets are frequently associated with age. Here, we sought to understand baseline patient, immune and viral biomarkers associated with the development of ICB-hepatitis to identify currently lacking baseline predictors and test if an expansion of TEM or positive serology against herpesviridae can predict ICB-hepatitis.
Methods
A discovery (n = 39) and validation cohort (n = 67) of patients with advanced melanoma undergoing anti-PD-1&anti-CTLA4 combination therapy (total n = 106) were analyzed for baseline clinical characteristics, occurrence of irAE and oncological outcomes alongside serological status for CMV, EBV and HSV. Immune populations were profiled by high-parametric flow cytometry (n = 29).
Results
ICB-hepatitis occurred in 59% of patients within 100days; 35.9% developed severe (CTCAE 3–4) hepatitis. Incidence of ICB-hepatitis was higher in the younger (< 55y: 85.7%) compared to older ( > = 55y: 27.8%) age group (p = 0.0003), occured earlier in younger patients (p < 0.0001). The association of younger age with ICB-Hepatitis was also observed in the validation cohort (p = 0.0486). Incidence of ICB-hepatitis was also associated with additional non-hepatic irAE (p = 0.018), but neither positive IgG serostatus for CMV, EBV or HSV nor TEM subsets despite an association of T cell subsets with age.
Conclusion
Younger age more accurately predicts ICB-hepatitis after anti-PD-1&anti-CTLA4 checkpoint therapy at baseline compared to herpes virus serology or TEM subsets. Younger patients should be carefully monitored for the development of ICB-hepatitis.
Transcriptome Analysis Reveals Auto-Inflammatory Genes of Chronic Nonbacterial Osteomyelitis (CNO) Based on The Machine Learning
Authors: Fu Zhuodong , Wang Xingkai , Zou Linxuan , Zhang Zhe , Lu Ming , Zong Junwei , Wang Shouyu 
Published: October 2022

Abstract
Objectives:
Chronic nonbacterial osteomyelitis (CNO) is an auto-inflammatory bone disorder. Since the origin and development of CNO involve many complex immune processes, resulting in delayed diagnosis and lack of effective treatment. Although bioinformatics analysis has been utilized to seek key genes and pathways of CNO, only a few bioinformatics studies that focus on CNO pathogenesis and mechanisms have been reported. This study aimed to identify key biomarkers that could serve as early diagnostic or therapeutic markers for CNO.
Methods:
Two RNA-seq datasets (GSE133378 and GSE187429) were obtained from the gene expression omnibus (GEO). Weighted gene co-expression network analysis (WGCNA) and differentially expressed genes (DEGs) analysis were conducted to identify the correlated genes associated with CNO. After that, the auto-inflammatory genes mostly associated with CNO were yielding based on the GeneCards database and the CNO prediction model, which was created by the LASSO machine learning algorithms. According to the receiver operating characteristic (ROC) curves, the accuracy of the model and auto-inflammatory genes was verified by utilizing external datasets (GSE7014). Eventually, we performed clustering analysis by ConsensusClusterPlus.
Results:
Totally, eighty CNO-related genes were identified, which were significantly enriched in the biological process of regulation of actin filament organization, cell-cell junction organization and gamma-catenin binding. The mainly enriched pathways were Adherens junction, Viral carcinogenesis and Systemic lupus erythematosus. Two auto-inflammatory genes with high expression in CNO samples were identified by combing an optimal machine learning algorithm (LASSO) with GeneCards database. The external validation dataset (GSE187429) was utilized for ROC analysis of prediction model and two genes, and the results have well validation efficiency. Then, we found that the expression of the two genes differed between clusters based on consensus clustering analysis. Finally, the ceRNA network of lncRNA and small molecule compounds of the two auto-inflammatory genes were predicted.
Conclusion:
Two auto-inflammatory genes, HCG18/has-mir-147a/UTS2/MPO axis and the signal pathways identified in this study can help us understand the molecular mechanism of CNO formation and provide candidate targets for the diagnosis and treatment of CNO.
Adherence to Baltic Sea diet and healthy Nordic dietary pattern and lower risk of age-related cataract: a case-control study from Iran
Authors: Rahmani Jamal , Nikkhah Mehrnaz , Clark Cain C. T. , Ghanavati Matin 
Published: October 2022

Abstract
Background
Age-related cataract (ARC) is a multifactorial and progressive disease that causes blindness globally. Dietary antioxidants like vitamins and carotenoids have been reported to attenuate oxidative stress the main cause of ARC. However, the association between adherence to dietary healthy dietary patterns and ARC has yet to be understood. Since, adherence to Nordic diet style measured by two indices including Healthy Nordic Food Index (HNFI) and Baltic Sea Dietary Score (BSDS) was associated to some chronic diseases, in this study we aimed to investigate the association between HNFI and BSDS and the odds of ARC in a case-control study, in Iran.
Methods
This hospital-based case-control study was conducted on 98 patients with cataract and 198 healthy controls selected based inclusion criteria. A valid 168-item food frequency questionnaire (FFQ) was used to assess dietary intake over last year. To investigate the association between HNFI and BSDS and risk of ARC, the logistic regression tests was performed.
Results
Individuals in case and control groups were significantly different in body mass index (BMI), history of diabetes, hypertension and family history of hypertension. The intake of vitamin C, vitamin A and vitamin D showed significant difference between two groups of study. The adherence to BSDS and HNFI were associated with lower risk of ARC in crude and adjusted models. After adjustment for potential cofounders, participants in the highest tertile of HNFI and BSDS (compared to the lowest tertile) had 99.1% and 74% lower ARC risk (OR = 0.09; 95% CI = 0.03–0.22 and OR = 0.26; 95% CI = 0.11–0.58, respectively).
Conclusion
Our results support previous findings on protective effects of dietary patterns rich in antioxidant on cataract. Adherence to Nordic eating style measured by BSDS and HNFI consist of fruits and root vegetables, whole grain, fish and health fats may reduce risk of ARC.