bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐cell transcriptomic atlas of different endometriosis indicating that an interaction between endometriosis‐associated mesothelial cells (EAMCs) and ectopic stromal cells may influence progesterone resistance
Authors: Hou Shengdi , Zhang Jing , Zhang Zhiqiang , Qu Hong , Li Shuhong , Jiang Ying , Liu Chongdong 
Published: February 2025

AbstractBackgroundEndometriosis is a hormone‐dependent disease, which can usually be divided into peritoneal endometriosis (PEM), deep‐infiltrating endometriosis (DIE) and ovarian endometriosis (OEM). Although the three pathologic types are essentially the same disease, they differ in pathological manifestations, molecular features, pain symptoms and hormonal responsiveness. However, there is limited literature focusing on the differences among these types. In this study, we employed single‐cell RNA sequencing (scRNA‐seq) to profile the transcriptome of each type using surgical biopsy samples obtained from six patients. We aimed to explore and elucidate the variations among these different types of endometriosis.ResultsWe identified five major cell types and 44 subpopulations, including the presence of mesothelial cells in all pathological types, including OEM. Furthermore, we characterised the variations in cell types across different pathological types by employing enrichment analysis to assess functions and pathways. Notably, our findings reveal distinct levels of epithelial–mesenchymal transition (EMT) processes experienced by mesothelial cells within the microenvironment of endometriotic lesions. Through ligand–receptor analysis and referencing relevant literature, we propose that mesothelial cells exert an influence on progesterone resistance in stromal cells through intercellular communication mediated by the FN1‐AKT pathway.ConclusionsOur study comprehensively characterises the heterogeneity of the different pathologic types of endometriosis and offers valuable insights into the underlying mechanisms contributing to variations in progesterone resistance across the three subtypes.Key points
Single‐cell RNA (ScRNA) atlas across types of endometriosis is established.
Mesothelial cells are founded in ovarian endometriosis.
Endometriosis‐associated mesothelial cells (EAMCs) experience various level of epithelial–mesenchymal transition (EMT) process in different subtypes.
EAMCs may exert an influence on progesterone resistance in stromal cells through intercellular communication mediated by the FN1‐AKT pathway.
Demographic effects of sanitary policies on European vulture population dynamics: A retrospective modeling approach
Authors: Colomer MªÀngels , Margalida Antoni 
Published: February 2025

AbstractThe prediction of population responses to environmental changes, including the effects of different management scenarios, is a useful tool and a necessary contributor to improving conservation decisions. Empirical datasets based on long‐term monitoring studies are essential to assess the robustness of retrospective modeling predictions on biodiversity. These allow checks on the performance of modeling projections and enable improvements to be made to future models, based on the errors detected. Here, we assess the performance of our earlier model to assess the impact of vulture food shortages caused by sanitary regulations on the population dynamics of Spanish vultures during the past decade (2009–2019). This model forecasts the population trends of three vulture species (griffon, Egyptian, and bearded vultures) in Spain (home to 90% of the European vulture population) under various food shortage scenarios. We show that it underestimated bearded and griffon vulture population numbers and overestimated Egyptian vultures. The model suggested that the most plausible food shortage scenario involved an approximate 50% reduction of livestock carcass availability in the ecosystem compared with the previous situation without sanitary carcass removal. However, the observed annual population growth for the period 2009–2019 (7.8% for griffon vulture, 2.4% for Egyptian vulture, and 3.5% for bearded vulture) showed that food shortages had little impact on vulture population dynamics. After assessing the robustness of the model, we developed a new model with updated demographic parameters and foraging movements under different hypothetical food shortage scenarios for the period 2019–2029. This model forecasts annual population increases of about 3.6% for the bearded vulture, 3.7% for the Egyptian vulture, and 1.1% for the Griffon vulture. Our findings suggest that food shortages due to the implementation of sanitary policies resulted in only a moderate impact on vulture population growth, probably thanks to the supplementary feeding network which provided alternative food. Also important was the availability of alternative food sources (intensive farms, landfills) that were used more regularly than expected. We discuss the computational performance of our modeling approach and its management consequences to improve future conservation measures for these threatened species, which provide essential ecosystem services.
Discovery of miRNAs unique to actively transcribed erythroparvovirus infection in heart failure patients
Authors: Aleshcheva Ganna , Salih Sara , Baumeier Christian , Escher Felicitas , Bock C. Thomas , Schultheiss Heinz‐Peter 
Published: February 2025

AbstractAimsmiRNAs, small non‐coding RNAs, play key roles in gene regulation, cell differentiation and tissue development. They influence viral infection outcomes by directly interacting with viral genomes or modifying the host microenvironment. This study demonstrates miRNAs' ability to selectively suppress transcriptionally active erythroparvovirus, highlighting their potential in antiviral therapies.Methods and resultsSeventy‐five endomyocardial biopsy (EMB) specimens from patients with unexplained heart failure were analysed. The samples included 19 with dilated cardiomyopathy and inflammation (DCMi), 12 with dilated cardiomyopathy (DCM), 25 with inflammation and active erythroparvovirus infection, 13 with active erythroparvovirus infection only and 6 from undiagnosed patients as controls. miRNA expression was measured using TaqMan assays. miR‐98, miR‐222, miR‐106b and miR‐197 were significantly upregulated in patients with transcriptionally active erythroparvovirus infection, independent of inflammation (P < 0.005). These miRNAs differentiated these patients from all other groups with over 90% specificity.ConclusionsThese specific miRNAs offer a novel diagnostic tool for active erythroparvovirus infections and hold promise as therapeutic targets, providing safer alternatives to traditional antiviral treatments.
A N3‐geminal Al/P‐based Intramolecular Lewis Pair: Synthesis, Characterization and Reactivity
Authors: Sun Xiong , Xie Zhuoyi , Zou Lianghua 
Published: February 2025

Frustrated Lewis pairs (FLPs) play a crucial role in main‐group chemistry due to the diverse reactivities towards small molecules and extensive applications in organic transformations. The intramolecular FLP systems known in the literature are mainly consisted of boron‐containing Lewis acids and the linkers are almost exclusively based on carbon. Herein, the nitrogen‐linked geminal Al/P Lewis pair 1 was readily prepared by salt metathesis reaction between AlBr3 and N[CH2CH2NLi‐PtBu2]3 in toluene at room temperature. Further studies confirmed that 1 exhibited typical FLP‐type reactivity towards heterocumulenes, isocyanide and transition metal precursors. Single‐crystal X‐ray diffraction analysis revealed that all the addition products contained a penta‐coordinated aluminum atom which was less common in FLP chemistry. This study demonstrated that the resulting Al/P‐based system represented an efficient alternative class of FLPs and enriched the linker motifs of intramolecular FLPs.
Automated and Parallel Amide Synthesis
Authors: Jiang Tuo , Coin Guillaume , Bordi Samuele , Nichols Paula L. , Bode Jeffrey W. , Wanner Benedikt M. 
Published: February 2025

AbstractAmide bonds are ubiquitous in molecules of interest such as pharmaceuticals, natural products, and agrochemicals. Over the last decades, significant efforts have been directed at developing efficient methods for the formation of this class of chemical bonds. Herein, we disclose a robust, user‐friendly approach for the efficient and rapid parallel synthesis of amide‐containing compounds, in good yield and purity. Our approach utilizes automated synthesis technologies and pre‐packed capsules containing all the necessary materials for the reaction and work‐up. Following manual addition of the amine and carboxylic acid, the reaction and product isolation is achieved automatically in a standardized, integrated manner. This methodology tolerates a wide diversity of carboxylic acids and amines (or amine salts). To accelerate discovery and rapidly create amide‐containing compound libraries, the method was miniaturized into an automated parallel synthesis format, using 96‐well plate kits, which could be beneficial to medicinal chemists.
The Effect of a Single Session Rubber Hand Illusion on Pressure Pain Is Not Long‐Lasting
Authors: Ceylan Hatice , Acet Nagihan , Günendi Zafer 
Published: February 2025

ABSTRACTBackgroundRubber hand illusion (RHI) is an experience that causes changes in body perception and awareness as a result of the integration of simultaneous perceived visual and tactile stimuli. After synchronous brush strokes with rubber and real hands, the person perceives the rubber hand as their own. RHI is known to alter pain perception. In this study, it was aimed to evaluate the effects of RHI on pressure pain threshold and continuity of this effect.MethodsTwenty‐three volunteers who developed RHI were included in our study and two conditions, illusion (synchronous) and control (asynchronous), were applied. The illusion condition was created by synchronous brush strokes, while the control condition was created by asynchronous brush application using different frequency and different finger areas in the same individuals. In both conditions, pressure pain threshold measurements with an algometer were performed at four times: baseline/1st measurement, during the brush stroke/2nd measurement, at the end of the brush stroke/3rd measurement and after the hand was removed from the environment/4th measurement.ResultsIt was shown that RHI increased the pressure‐pain threshold (p = 0.004) in healthy volunteers. Asynchronous brush strokes arranged as a control trial significantly decreased the pressure pain threshold (p = 0.002).ConclusionsIt was found that the threshold values that change during the brush strokes return to the initial state after the brush strokes are terminated and the rubber hand is removed from the environment so that the effect of the illusion does not last for a long time with a single session application.
Diagnostic yield of utilizing 24–72‐hour video electroencephalographic monitoring in the diagnosis of seizures presenting as paroxysmal events in resource‐limited settings
Authors: Nwogu Chukwudi , Nwaze Chukwuma , Avah Adaeze , Anyanwu Benjamin , Anyanwu Chinekwu 
Published: February 2025

AbstractObjectiveLong‐term video electroencephalogram monitoring (LTVEM) is a standard practice in epilepsy centers to diagnose and characterize paroxysmal events. With the lack of data on LTVEM in Africa, we aimed to determine the clinical yield of LTVEM performed for 24–72 h to diagnose seizures in an epilepsy center in Nigeria.MethodsThis was a retrospective review of all patients admitted to our Epilepsy Monitoring Unit (EMU) from September 2018 to September 2021, with monitoring lasting between 24 and 72 h. We reviewed the patients' seizure semiology, time to the first event, and final diagnosis. The frequency of seizures was classified as “daily” in patients with one or more seizures per day, “persistent” in patients with less than one seizure per day but at least once in 6 months, and “rare” in patients with less than one seizure in 6 months. Patients with unclear duration due to recent onset were classified as “undefined”.ResultsSeventy patients (34 males, 36 females) were included in our study. The mean age was 22.86 ± 18.00 years. The average duration of monitoring was 44.23 ± 16.16 h. Fifty‐seven patients (81.4%) were confirmed to have seizures. Thirteen patients experienced non‐epileptic events. Of these 13 patients, nine were diagnosed with psychogenic non‐epileptic spells (PNES). Two patients were diagnosed with essential myoclonus and two patients were diagnosed with syncope. The time to the first interictal epileptiform discharge was within 8 h. In the first 24 h, 56 of 57 patients had ictal and interictal discharge (98.2%). These included 100% daily seizures, 100% persistent seizures, and 100% undefined events. One rare seizure was observed within 48 h.SignificanceThe diagnostic yield of the LTVEM in well‐selected patients for seizures in this study is 81.4%. Most patients received a diagnosis within 48 h of monitoring, and we found that extending the study beyond 72 h may not offer significant additional benefits in diagnosing seizures in patients presenting with paroxysmal events.Plain Language SummaryThere is limited information about the usefulness of long‐term video electroencephalogram (EEG) monitoring in diagnosing seizures in Africa. Several conditions that resemble seizures (paroxysmal events) could be misdiagnosed, leading to inappropriate treatment. This study evaluated the effectiveness of 24‐ to 72‐h video EEG monitoring in diagnosing seizures at an epilepsy center in Nigeria. Among 70 patients, 81.4% were diagnosed with seizures, with most diagnoses made within 48 h. The findings suggest that video EEG performed within 48 h can significantly help distinguish seizures from other paroxysmal events, thereby contributing to better management and outcomes.
Impacts of Nonlinear Loads on the Power Quality of Solar Microgrids and Proposed Mitigation Strategies
Authors: Tweve Exaud , Kichonge Baraka , Kivevele Thomas 
Published: February 2025

ABSTRACTThe integration of solar energy into microgrids poses significant challenges in maintaining power quality due to the variable nature of solar generation and the presence of nonlinear loads such as uninterruptible power supplies (UPS), variable frequency drives (VFDs), rectifiers, and power electronic components. Active power filters are often used to mitigate harmonics; however, these solutions are complex due to the use of control techniques like the synchronous reference frame and can introduce additional harmonic currents during compensation within electrical systems. This study investigates the effects of different nonlinear loads on the power quality of solar microgrids and explores the reduction of harmonics from VFDs through the application of passive harmonic filters (PHFs). The objective was achieved by measuring harmonics generated by nonlinear loads and simulating a modeled VFD circuit using MATLAB/Simulink R2021b software, considering the demand side. Analysis of current harmonics measurements at the point of common coupling revealed that the total harmonic current distortion (THCD) for UPS, VFDs, and rectifiers was 20.64%, 42.82%, and 22.59%, respectively, while the total harmonic voltage distortion (THVD) for UPS, VFDs, and rectifiers was 27.83%, 31.55%, and 29.16%, respectively. Moreover, the total losses caused by harmonic distortion from these nonlinear loads is 860 Watts. Among these loads, VFDs were the dominant source of harmonics. Therefore, the application of passive harmonic filters (PHFs) was proposed to minimize harmonics in solar microgrids, adhering to the standards set by the Institution of Electrical and Electronics Engineers (IEEE) and the International Electro‐Technical Commission (IEC). Simulation of the VFD revealed that the THCD was reduced by 89.4% and the THVD was minimized by 89.9% due to the connection of passive harmonic filters. These filters have the capability to minimize harmonics to within IEEE 519 standard limits.
Optimization of Coal Mining Machine Drum Structure and Microscopic Analysis of Coal Particle Flow
Authors: Lao Junming , Chen Zhi , Wang Xuecheng , Zhang Mengqi , Guo Jianfeng 
Published: February 2025

ABSTRACTTo solve the problems of low loading performance and insufficient design of the drum of thin‐seam coal mining machine, this paper proposes an innovative coal‐guiding drum design and systematically investigates the effects of different designs on the trajectory, flow structure, and loading efficiency of the coal particles from both macro‐ and microscopic perspectives. At the macroscopic level, the optimized design significantly improves the coal loading efficiency of coal particles by adding circular arc‐shaped coal guide plate and triangular coal guide plate. Simulation experiments show that the average mass flow rate of the coal‐guided drum is increased by 14.1%, and the mass flow rate under high load conditions is increased by 9.1%; with the increase of the rotational speed, the maximum increase of the loading efficiency is 16.96%. At the microscopic level, the optimized design changed the movement direction of coal particles, which led to a 15.85% increase in axial velocity and optimized the flow structure of coal particles. This paper provides a new theoretical basis and technical solution for improving the loading efficiency of thin‐seam coal‐mining machine, which has important practical significance and wide application potential.
Donepezil Reverses Alcohol‐Induced Changes in Hippocampal Neurogenic and Glial Responses Following Adolescent Intermittent Ethanol Exposure Into Adulthood in Female Rats
Authors: Nwachukwu Kala N. , Nelson James C. , Hill Kennedy M. , Clark Kennedy A. , Healey Kati , Swartzwelder H. Scott , Marshall S. Alex 
Published: February 2025

ABSTRACTAdolescent intermittent ethanol (AIE) exposure leads to persisting increases in glial markers and significantly decreases the neurogenic niche in the dentate gyrus of the hippocampus. Our previous study indicated that donepezil (DZ), a cholinesterase inhibitor, can reverse the AIE effect of decreased doublecortin (DCX), a neurogenic marker, and increased cleaved caspase 3, a marker of apoptosis, in the dentate gyrus of male rats. However, to date, no studies have assessed the effects of DZ on AIE effects in females. The purpose of this study was to determine whether DZ can reverse neuroimmune, neurogenic, and neuronal death effects in adulthood after AIE in female rats. Adolescent female rats were given 14 doses of ethanol (5 g/kg) over 24 days by intragastric gavage. Seventeen days later, DZ (2.5 mg/kg, 1.88 mL/kg, i.g., in water) was then administered daily for 4 days prior to sacrifice. Immunohistochemical techniques were utilized to determine the effects of DZ on AIE‐induced changes in neurogenesis, cell death, glial, and neuroimmune markers. As expected, AIE decreased the neurogenic markers DCX, SOX2, and Ki‐67 in the dentate gyrus and also caused an increase in the glial markers GFAP and Iba‐1 in the hippocampus. The effects of AIE on neurogenic and glial markers were reversed by DZ treatment, but the reversal of AIE effects on glial markers was regionally specific within the hippocampus. Overall, these findings indicate that systemic DZ in adult female rats ameliorates the effects of AIE on neurogenesis, neuronal cell death, neuroimmune markers, and glial activation markers. Future studies will determine if DZ alters hippocampally driven behaviors, as well as the mechanisms underlying donepezil's effects.
Twenty Four‐Hour Movement Behaviours Research Among Australian Children and Adolescents: A Scoping Review
Authors: Hossian Mosharop , Nisar Mehwish , Mielke Gregore Iven , Khan Asaduzzaman 
Published: February 2025

ABSTRACTBackgroundAdherence to 24‐h movement behaviour (24‐h MB) guidelines, encompassing moderate‐to‐vigorous physical activity (MVPA), recreational screen time (ST) and sleep, is low among Australian children and adolescents, with poorly understood contributing factors. This review synthesised current evidence to identify areas requiring further exploration in this demographic.MethodsA systematic search in PubMed, Scopus, Web of Science, SportDiscus and CINAHL identified articles published from June 2016 and March 2024. Peer‐reviewed articles in English focusing on healthy school‐aged children and adolescents (5–17 years) addressing 24‐h MB guidelines, including those using compositional data analysis (CoDA), were included.ResultsTwenty‐three articles met the inclusion criteria. Most were of fair quality and used cross‐sectional designs and self‐reported measures. Adherence to 24‐h MB guidelines ranged from 2% to 22% in Australia. The included articles focused on prevalence (n = 12), correlates (n = 5), health outcomes (n = 13), academic performance (n = 4), and an intervention (n = 1). Factors negatively associated with adherence included lower socioeconomic status, transitions from primary to secondary school, and family history of diabetes. Positive outcomes associated with adherence included improved academic performance, quality of life, and physical and mental health. Important research gaps were identified, particularly regarding intervention studies and limited exploration of a causal relationship between adherence, contributing factors, and related outcomes. Compositional analyses (n = 10) suggested that reallocating time from sedentary behaviour to MVPA may improve fitness and academic performance, whereas increasing sedentary time may lower sleep efficiency.ConclusionsA comprehensive understanding of factors associated with adherence to 24‐h MB guidelines among Australian children and adolescents is lacking. More longitudinal and interventional studies using objective measures are needed to establish causality and provide a deeper understanding of adherence to 24‐h MB guidelines among children and adolescents in Australia.So WhatThis scoping review underscores the need for more longitudinal and intervention‐based research to explore causal relationships between adherence to 24‐h MB guidelines and potential benefits for health, academic performance, and quality of life in Australian children and adolescents.
Bank Capital Regulation and Derivatives Clearing
Authors: Acosta‐Smith Jonathan , Ferrara Gerardo , Rodriguez‐Tous Francesc 
Published: February 2025

ABSTRACTAs part of the post global financial crisis banking reforms, regulators introduced a leverage ratio requirement, a minimum capital requirement over a bank's total exposures. We assess the consequences of this requirement for derivative clearing services to clients, which creates exposures for the dealers, by exploiting its earlier introduction in the United Kingdom and detailed confidential transaction and portfolio data. UK dealers reduce their client clearing business following the introduction of the requirement, particularly dealers with lower capital positions and for longer‐term derivatives; they are also more likely to end client relationships. Capital regulation might indirectly limit access to clearing services.