bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promoting community-based surveillance of economically important invasive species in lower-income economies: a case study of the suckermouth catfish (Pterygoplichthys pardalis) in Bangladesh
Authors: Mamun SM Abdullah Al , Hossain Mohammad Sorowar , Capps Krista 
Published: August 2022

Abstract
The distribution of invasive species in lower-income economies is relatively poorly understood, and many countries do not actively curate a list of species invasions or have programs to monitor or mitigate the effects of non-native species within their borders. Methods leveraging new technologies and expanding access to the internet may offer the opportunity to support low-cost and large-scale monitoring efforts. Here, we demonstrate how the use of online news coverage can support initial attempts to map the distribution of an economically-important, non-naïve freshwater fish in Bangladesh (Ptergoplichthys sp.). By initiating new collaborations between the scientific and online journalism communities, governments and organizations may be able to enhance their abilities to map the distribution of non-native species and develop new programs to support public awareness of species invasion at relatively low cost.
Left-sided Valvular Heart Disease and Survival in Out-of-Hospital Cardiac Arrest: A Nationwide Registry-Based Study
Authors: Dejby Ellen , Bhatt Deepak L. , Skoglund Kristofer , Rawshani Aidin , Omerovic Elmir , Redfors Björn , Myredal Anna , Petursson Petur , Angerås Oskar , Gustafsson Arvid , Isaksén Daniella , Herlitz Johan , Rawshani Araz 
Published: August 2022

Abstract
Background
Survival in left-sided valvular heart disease (VHD; aortic stenosis [AS], aortic regurgitation [AR], mitral stenosis [MS], mitral regurgitation [MR]) in out-of-hospital cardiac arrest (OHCA) is unknown.
Methods
We studied all cases of OHCA in the Swedish Registry for Cardiopulmonary Resuscitation. Association between VHD and survival was studied using logistic regression, gradient boosting and Cox regression. We studied time to cardiac arrest, comorbidities, survival, and cerebral performance category (CPC) score.
Results
We included 55,615 patients; 1948 with AS, 384 AR, 17 MS, and 704 with MR. Time from VHD diagnosis to cardiac arrest was 3.7 years in AS, 4.5 years in AR and 4.1 years in MR. ROSC occurred in 28% with AS, 33% with AR, 36% with MR and 35% without VHD. Survival at 30 days was 5.2%, 10.4%, 9.2%, 11.4% in AS, AR, MR and without VHD, respectively. There were no survivors in people with AS presenting with asystole or PEA. CPC scores did not differ in those with VHD compared with no VHD. Odds ratio (OR) for MR and AR showed no difference in survival, while AS displayed OR 0.58 (95% CI 0.46–0.72), vs no VHD.
Conclusion
AS is associated with halved survival in OHCA, while AR and MR do not affect survival. Survivors with AS have neurological outcomes comparable to patients without VHD.
Cancer subclone detection based on DNA copy number in single cell and spatial omic sequencing data
Authors: Zhang Nancy , Wu Chi-Yun , Sathe Anuja , Rong Jiazhen , Hess Paul , Lau Billy , Grimes Susan M , Ji Hanlee 
Published: August 2022

Abstract
In cancer, somatic mutations such as copy number alterations (CNAs) accumulate during disease progression and lead to functional intra-tumor heterogeneity that can influence the efficacy of cancer therapy. Therefore, studying the functional characteristics and spatial distribution of genetically distinct subclones is crucial to the understanding of tumor evolution and the design of cancer treatment. Here, we present Clonalscope, a method for subclone detection using copy number profiles that can be applied to spatial transcriptomics (ST) data and data from single-cell sequencing platforms such as scRNA-seq and scATAC-seq. Clonalscope implements a nested Chinese restaurant process to identify de novo subclones within one or multiple samples from the same patient. Clonalscope incorporates prior information from paired whole-genome or whole-exome sequencing (WGS/WES) data to achieve more reliable subclone detection and malignant cell labeling. On scRNA-seq and scATAC-seq data from four gastrointestinal tumor samples, Clonalscope successfully labeled malignant cells and identified genetically different subclones, which were validated in detail using matched scDNA-seq data. On ST data from a squamous cell carcinoma and two invasive ductal carcinoma samples, Clonalscope successfully labelled malignant spots, traced subclones between associated datasets, and identified spatially segregated subclones expressing genes associated with drug resistance and survival.
Subthalamic beta bursts correlate with dopamine-dependent motor symptoms in 106 Parkinson's patients
Authors: Lofredi Roxanne , Okudzhava Liana , Irmen Friederike , Brücke Christof , Huebl Julius , Krauss Joachim , Schneider Gerd-Helge , Faust Katharina , Neumann Wolf-Julian , Kühn Andrea 
Published: August 2022

Abstract
Pathologically increased beta power has been described as a biomarker for Parkinson’s disease (PD) and related to prolonged bursts of subthalamic beta synchronization. Here, we investigate the association between subthalamic beta dynamics and motor impairment in a cohort of 106 Parkinson’s patients in the ON- and OFF- medication state, suing two different methods of beta burst determination. We report a frequency-specific correlation of low beta power and burst duration with motor impairment OFF dopaminergic medication. Furthermore, reduction of power and burst duration correlated significantly with symptom alleviation through dopaminergic medication. Importantly, qualitatively similar results were yielded with two different methods of beta burst definition. Our findings validate the robustness of previous results on pathological changes in subcortical oscillations both in the frequency- as well as in the time-domain in the largest cohort of PD patients to date with important implications for next-generation adaptive deep brain stimulation control algorithms.
Tubular aryl hydrocarbon receptor upregulates EZH2 to promote cell senescence in cisplatin-induced acute kidney injury
Authors: Wen Li , Ren Qian , Du Xiaoyan , Yang Hongliu , Guo Fan , Ma Liang , Fu Ping 
Published: August 2022

Abstract
Acute kidney injury (AKI) is one of the serious clinical syndromes with high morbidity and mortality. Despite substantial progress in understanding of AKI mechanism, no drug therapy is available for treatment or prevention. In this study, we identified that a ligand-activated transcription factor aryl hydrocarbon receptor (AhR) was highly expressed in the kidneys of cisplatin-induced AKI mice or tubular epithelial TCMK-1 cells. Notably, the AhR inhibition by BAY2416964 and tubular conditional deletion both alleviated cisplatin-induced kidney injury and cellular senescence, which was indicated by senescence-associated β-galactosidase (SA-β-gal) activity, biomarker p53, p21, p16 protein expression, and secretory phenotype IL-1β, IL-6 and TNFα level. Mechanistically, the abnormal AhR expression was positively correlated with the elevated EZH2, a methyltransferase. In addition, AhR inhibition also could suppress the EZH2 expression in the cisplatin-injured kidneys. Furthermore, the ChIP assay displayed that EZH2 may indirectly interact with AhR promoter region by affecting H3K27me3. The direct recruitment between H3K27me3 and AhR promoter is higher in the kidneys of control than that of cisplatin mice, suggesting EZH2 could reversely regulate the AhR expression through weakening H3K27me3 transcriptional inhibition on AhR promoter. Collectively, the present study implicated that AhR and EZH2 have mutual regulation, which further accelerated tubular cellular senescence in cisplatin-induced AKI.
CENPF Knockdown Inhibits Adriamycin Chemoresistance in Triple Negative Breast Cancer mediated by Rb-E2F1 Axis
Authors: Wang Depeng , Xu Wei , Huang Minghua , Ma Wei , Liu Yulu , Zhou Xingchen , Yang Qingrui , Mu Kun 
Published: August 2022

Abstract
Background:
Frequent developed resistance leads to early relapse and short survival in Triple-negative breast cancer (TNBC). Targeting DNA damage response (DDR) has become an effective strategy for overcoming TNBC chemoresistance. CENPF (centromere protein) is a key regulator of cell cycle progression, but its role in TNBC chemotherapy resistance remains unclear.
Methods:
We Used bioinformatics, immunohistochemical staining and real-time quantitative PCR analysis to explore the expression of CENPF in triple negative breast cancer tissues and cells. Then, we analyze the relationship between CENPF expression with clinicopathological parameters and survival prognosis of patients. The effects of CENPF on the proliferation, apoptosis, cycle, and drug sensitivity of triple negative breast cancer cells were detected in vitro. The chemoresistance mechanisms of CENPF in TNBC cells was evaluated by immunoblottin, immunofluorescence co-localization, and co-immunoprecipitation .
Results:
Here we found CENPF, highly expressed in TNBC, is associated with poor prognosis in patients receiving chemotherapy. And in vitro CENPF knockdown significantly increased adriamycin (ADR)-induced cytotoxicity in MDA-MB-231 and its ADR-resistant strains (MDA-MB-231/ADR). Then we demonstrated that CENPF targets Chk1-mediated G2/M phase arrest and binds to Rb for competing with E2F1 in TNBC. Collectively Considering crucial role of E2F1 in DNA damage response and DNA repair, a novel mechanism for CENPF to regulate Rb-E2F1 axis will provide new horizons to finally overcome chemotherapy resistance in TNBC.
Conclusion:
CENPF binds to Rb for competing with E2F1 and promotes Chk1 expression through the Rb/E2F1 axis to enhance chemoresistance to doxorubicin in TNBC.
Hybrid immunity at 6 months after SARS-CoV-2 exposure in individuals from the community treatment program
Authors: Chevaisrakul Parawee , Lumjiaktase Putthapoom , Kietdumrongwong Pongtorn , Chuatrisorn Ittiporn , Chatsangjaroen Pongsan , Phanuphak Nittaya 
Published: August 2022

Abstract
The death rate from SARS-CoV-2 infection (COVID-19) was lowered from 2021 to 2022, while the infection rate was increasing. Hybrid immunity, caused by vaccination and infection, including asymptomatic infection, may confer effective protection against death. We explored the combined effect of asymptomatic COVID-19 and hybrid immunity by studying T cell and antibody responses against SARS-CoV-2 in individuals treated in home healthcare service at 6 months after COVID-19.ResultsAsymptomatic COVID-19 was demonstrated in 24.4% of closed contacts. The levels of immunity were comparable between COVID-19 patients and close contacts. Anti RBD IgG against SARS-CoV-2 virus increased dose dependently to the numbers of vaccination, in contrast to T cell response that decreased at early period after vaccine booster.ConclusionsAsymptomatic COVID-19 may not contribute to immunity against SAR-CoV-2 developed in vaccinated close contacts. However, when designing vaccine strategies, T cell exhaustion after multiple vaccinations is a point of concern.
ANGPTL4 is a potential driver of HCV-induced peripheral insulin resistance
Authors: Gomes Diana , Sobolewski Cyril , Conzelmann Stéphanie , Schaer Tifany , Lefai Etienne , Alfaiate Dulce , Tseligka Eirini D. , Goossens Nicolas , Negro Francesco , Foti Michelangelo , Clément Sophie 
Published: August 2022

Abstract
Chronic hepatitis C (CHC) is associated with the development of metabolic disorders, including both hepatic and extra-hepatic insulin resistance (IR). Here, we aimed at identifying liver-derived factor(s) potentially inducing peripheral IR and uncovering the mechanisms whereby HCV can regulate the action of these factors. We found ANGPTL4 (Angiopoietin Like 4) mRNA expression levels to positively correlate with HCV RNA (r = 0.46, p < 0.03) and HOMA-IR score (r = 0.51, p = 0.01) in liver biopsies of lean CHC patients. Moreover, we observed an upregulation of ANGPTL4 expression in two models recapitulating HCV-induced peripheral IR, i.e. mice expressing core protein of HCV genotype 3a (HCV-3a core) in hepatocytes and hepatoma cells transduced with HCV-3a core. Treatment of differentiated myocytes with recombinant ANGPTL4 reduced insulin-induced Akt-Ser473 phosphorylation. In contrast, conditioned medium from ANGPTL4-KO hepatoma cells prevented muscle cells from HCV-3a core induced IR. Treatment of HCV-3a core expressing HepG2 cells with PPARγ antagonist resulted in a decrease of HCV-core induced ANGPTL4 upregulation. Together, our data identified ANGPTL4 as a potential driver of HCV-induced IR and may provide working hypotheses aimed at understanding the pathogenesis of IR in the setting of other chronic liver disorders.
Risk of Falls and Associated Factors Among Older Colombian Adults: A Secondary Sabe Analysis
Authors: Díaz-Vidal Diana , Tadger Philippe , Quino-Ávila Aura Cristina , Sandoval-Cuellar Carolina , Alfonso-Mora Margareth Lorena , Monsalve-Jaramillo Eliana 
Published: August 2022

Abstract
Introduction: Falls among the elderly are involuntary events that have a negative impact on their health, necessitating identification of their risk. Objective: To determine which factors are associated with the risk of falling in older Colombian adults. Methods: This was a secondary analysis of the SABE-Colombia study of 18,049 older adults, where the risk of falling was determined using the Downton scale and its association was investigated with variables such as age, height, weight, weight loss, alcohol consumption (in days), years of tobacco use, calf circumference, Barthel Index, instrumental activities of daily living (IADL), signs and symptoms, fear of falling, and architectural barriers outside and inside the home. The Lasso method was used to perform multiple analyses on the selected variables. Results: Being independent in performing basic ADL, as measured by the Barthel Index [odds ratio (OR): 92%], the absence of dangers at home (OR: 98%), needing assistance for medication management (OR: 66%) and transportation (OR: 83%), having a weight above the average of 78.15 kg (OR: 99%), and fewer years as a smoker (OR: 99%) showed no positive association with the risk of falling. Conclusions: Age, back pain, calf circumference, difficulty sleeping, dizziness, fear of falling, needing assistance walking 400 m, shortness of breath while awake, and tiredness are the variables associated with the risk of falling.
Increased stride variability may predict lower leg overuse injuries among infantry soldiers
Authors: Nesterovica Darja , Vaivads Normunds , Stepens Ainars 
Published: August 2022

Abstract
Background A high incidence of lower leg overuse injury has been observed among military populations. Footwear usage could be promising in reducing the risk of injury from overuse of the lower extremities. The purpose of this study was to identify possible gait-related risk factors for lower leg overuse injuries when walking barefoot and in military boots. MethodsCases (n=32) were active-duty infantry soldiers from Latvian Land Forces with a history of overuse injury during the last 6 months of service before entering the study. During the study period, the cases recovered completely from the injury, did not report any functional limits, and were able to participate in all kinds of physical activities. Controls (n=32) of the same age and gender were free of injury in the same time period. Study participants were instructed to walk on a 5-meter walkway in the gait laboratory. Joint angle calculations and spatiotemporal gait parameters were evaluated for barefoot and shod conditions.Results Gait-related parameters during shod and barefoot conditions differ in both groups. The barefoot stride time and the stride time variability were statistically different between cases and controls. Conditional regression analysis showed that stride time variability during barefoot gait statistically significantly predicted the risk of lower leg overuse injury. ROC analysis showed an AUC of 0.77 (p<0.01; 95% CI 0.65-0.88), a sensitivity of 56%, and a specificity of 88%, with an optimal cut-off value for stride time variability of 1.95%.ConclusionsThe risk of injury was independent of the gait-related characteristics during shod gait. Stride time variability has low sensitivity, but if the variability value during the barefoot walk is less than 1.95%, it can be considered a negative risk factor for lower leg overuse injury among infantry soldiers.
Acupuncture efficacy for fibromyalgia in the framework of predictive, preventive, and personalized medicine: results of a pragmatic open-label study
Authors: Carlo Marco Di , Beci Giacomo , Cipolletta Edoardo , Salaffi Fausto 
Published: August 2022

Abstract
Background.
Acupuncture is an effective therapy in chronic pain that can be recommended in patients with fibromyalgia (FM). However, the symptomatological expressiveness of FM is very wide, and to date, there is no ideal patient who is a suitable candidate for acupuncture therapy. In a high-prevalence disease such as FM, in the absence of well-codified treatment patterns, being able to apply the rationale of predictive, preventive and personalized medicine (PPPM/3PM) is crucial to avoid unnecessary expense and burden on patients. The aim of this study, in the context of PPPM/3PM, is to identify the predictive factors of failure to acupuncture in order to identify the ideal FM patient to be directed to such treatment.
Methods.
FM patients with severe symptoms refractory to standard drug therapy underwent eight weekly acupuncture sessions. Significant improvement, defined as a reduction of at least 30% of the Revised Fibromyalgia Impact Questionnaire (FIQR), was assessed at the end of the eight weeks (T1) of treatment and three months after the end of treatment (T2). Univariate analysis was conducted to identify predictors of significant improvement at T1 and T2. Variables, which resulted significantly associated with clinical improvement at univariate analysis, were included in multivariate models.
Results.
Analyses were conducted on 77 patients (9 males, 11.7%). At T1, significant improvement in FIQR was recorded in 34 out of 77 patients (44.2%). At T2, significant improvement in FIQR persisted in 16 out of 77 patients (20.8%). In multivariate analysis, predictive variables of treatment failure were tender point count (odds ratio [OR] = 0.49, 95% confidence interval [95% CI]: 0.28–0.86, p = 0.01) and pain magnification (OR = 0.68, 95% CI: 0.47–0.99, p = 0.04) assessed with Pain Catastrophizing Scale, at T1. At T2, the only predictive variable of treatment failure was concomitant duloxetine use (OR = 0.21, 95% CI: 0.05–0.95, p = 0.04).
Conclusion.
The novelties of this study are in the context of PPPM/3PM in terms of a predictive approach, through the identification of clinical characteristics of unfavorable response to acupuncture. By evaluating these variables, it is possible to implement a targeted and cost-effective tertiary prevention and plan personalized medical service in patients with FM.
Chlorpromazine, an inverse agonist of D1R-like, differentially targets voltage-gated calcium channels (CaV) subtypes in mPFC neurons
Authors: McCarthy Clara Inés , Mustafá Emilio Román , Cornejo María Paula , Yaneff Agustin , Rodriguez Silvia Susana , Perello Mario , Raingo Jesica 
Published: August 2022

Abstract
The dopamine receptor type 1 (D1R) and the dopamine receptor type 5 (D5R), which are often grouped as D1R-like due to their sequence and signaling similarities, exhibit high levels of constitutive activity. The molecular basis for this agonist-independent activation have been well characterized through biochemical and mutagenesis in vitro studies. In this regard, it was reported that many antipsychotic drugs act as inverse agonists of D1R-like constitutive activity. On the other hand, D1R is highly expressed in the medial prefrontal cortex (mPFC), a brain area with important functions such as working memory. Here, we studied the impact of D1R-like constitutive activity and chlorpromazine (CPZ), an antipsychotic drug and D1R-like inverse agonist, on various neuronal CaV conductances and we explored its effect on calcium-dependent neuronal functions in the mouse medial mPFC. Using ex vivo brain slices containing the mPFC and transfected HEK293T cells, we found that CPZ reduces CaV2.2 currents by occluding D1R-like constitutive activity, in agreement with a mechanism previously reported by our lab; whereas CPZ directly inhibits CaV1 currents in a D1R-like activity independent manner. In contrast, CPZ and D1R constitutive activity did not affect CaV2.1 or CaV3 currents. Finally, we found that CPZ reduces excitatory postsynaptic responses in mPFC neurons. Our results contribute to understanding CPZ molecular targets in neurons and describe a novel physiological consequence of CPZ non-canonical action as a D1R-like inverse agonist in the mouse brain.
Modern technology adoption and professional development of Lecturers
Authors: Uzorka Afam , Namara Shiellah , Olaniyan Ademola 
Published: August 2022

Abstract
As technology becomes increasingly integrated with education, research regarding the professional development needs of lecturers making the transition to working in a technology-mediated environment following technological advancement is paramount in improving learning experiences. This study was carried out to investigate the lecturers’ professional development needs with regard to technology. This qualitative study randomly selected a convenience sample of 130 faculty and administrators. Using an interview guide, the interviewers met with 89 participants. Data were transcribed and entered into a database for analysis. The findings reported are uses of technology and professional development initiatives. Most lecturers identify time as their greatest barrier. Recognizing that time is also their most valued asset, lecturers look for professional development opportunities designed to meet their needs, relevant to how they want to apply the technology, with trainers and support people who use principles of andragogy and constructivism when teaching, training, and communicating. The study shows that there is a need for those involved in planning and implementing professional development opportunities to consider the needs of administrators and lecturers, and principles of adult education and constructivism when planning and implementing professional development opportunities.