bluuebunny/crossref_metadata_2025_split huggingface dataset:
Change in left atrioventricular coupling index to predict incident atrial fibrillation: the multi-ethnic study of atherosclerosis (MESA)
Authors: Pezel T , Ambale Venkatesh B , Quinaglia T , Heckbert S , Kato Y , De Vasconcellos H , Wu C , Post W , Henry P , Bluemke D , Lima J 
Published: February 2022

Abstract

Funding Acknowledgements
Type of funding sources: None.

BACKGROUND
Left atrial (LA) and left ventricular (LV) structural and functional parameters have independent prognostic values as predictors of atrial fibrillation (AF).

PURPOSE
To investigate the prognostic value of a left atrioventricular coupling index (LACI) and average annualized change in LACI measured by cardiac MRI to predict incident AF in a population-based sample from the Multi-Ethnic Study of Atherosclerosis (MESA).

METHODS
In a secondary analysis of the prospective Multi-Ethnic Study of Atherosclerosis (MESA) study, 1,911 study participants, free of clinically recognized AF and cardiovascular disease at baseline, had LACI assessed with cardiac MRI at baseline (Exam 1, 2000-2002), and ten years later (Exam 5, 2010-2012). LACI was defined as the ratio of LA to LV end-diastolic volumes. Univariable and multivariable Cox proportional hazard models were used to evaluate the associations of LACI and average annualized change in LACI (ΔLACI) with incident AF.

RESULTS
Among the 1,911 participants (mean age 59 ± 9 years and 907 men), 87 incident AF events occurred over 3.9 ± 0.9 years following the second imaging (Exam 5). After adjustment for traditional risk factors, greater LACI and ΔLACI were independently associated with AF (HR 1.69, 95% CI[1.46-1.96] and HR 1.71, 95% CI[1.50-1.94], respectively; both p&lt;.001). Adjusted models for LACI and ΔLACI showed improvement in model discrimination compared to currently used AF risk score (CHARGE-AF score) model (AUC: 0.78 vs. 0.74, and AUC: 0.80 vs. 0.74, both p&lt;.001); and to the final model including individual LA or LV parameters for predicting AF incidence (AUC: 0.78 vs. 0.76, and AUC: 0.80 vs. 0.78, both p&lt;.001).

CONCLUSIONS
Atrioventricular coupling (LACI) and coupling change (ΔLACI) were strong predictors for AF in a multi-ethnic population. Both had incremental prognostic value for predicting AF over traditional risk factors, and superior discrimination compared to the CHARGE-AF score and to individual LA or LV parameters.
ClinicalTrials.gov Identifier: NCT00005487 Abstract Figure. Kaplan-Meier curves by change in LACI Abstract Figure. Kaplan-Meier curves by ΔLACI and LACI
Short-term coronary risk assessment in the elderly population
Authors: Zagatina A , Kalinina E , Shmatov D 
Published: February 2022

Abstract

Funding Acknowledgements
Type of funding sources: None.
Background. Atherosclerosis and coronary artery disease (CAD) are a common condition and cause of death in the elderly population. There are difficulties with risk assessment in the elderly as the of their symptomatic status can be challenging due to neuromuscular weakness, physical deconditioning or neurological, orthopaedic, peripheral vascular, or respiratory limitations. Non-invasive coronary artery velocity assessment by Doppler method at rest could be helpful in the elderly population.

Aim
To evaluate the prognostic role of coronary artery ultrasound assessment in a non-selected elderly population in everyday clinical practice. Methods: 145 patients, aged ≥75years (99 women; 80 ± 4 years), formed the study group. Left coronary artery flows were scanned in addition to conventional echocardiography. During a median follow-up of 26 months, 16 deaths and 2 non-fatal MI occurred. Results: In multivariable analysis, maximal coronary velocity was the only independent predictor for mortality (HR: 1.02, 95%, CI: 1.01-1.04, p &lt; 0.0005), and for mortality/MI (HR: 1.02, 95%, CI: 1.01-1.03, p &lt; 0.0001). The value of 110 cm/s maximal coronary flow velocity in the proximal segments of left arteries was the best predictor for death, sensitivity 50%, specificity 90%, p &lt; 0.005. The annual mortality rate was 16.6% persons/year for patients with elevated coronary flow velocity ≥110 cm/s. The value 81 cm/s was the best predictor for death/MI, sensitivity 61%, specificity 80%, p &lt; 0.0005; annual mortality rate was 11.2% persons/year for patients with elevated coronary flow velocity ≥81 cm/s (p &lt; 0.0001).

Conclusion
Doppler coronary flow velocity scanning during routine echocardiography is a feasible and valuable tool for assessment of short-term prognosis in elderly patients. Abstract Figure.
Left atrial strain reduction in acute myocarditis and its association with incident atrial fibrillation
Authors: Pastore MC , Degiovanni A , Spinoni E , De Zan G , Carassia C , Patti G 
Published: February 2022

Abstract

Funding Acknowledgements
Type of funding sources: None.

Background
The diagnostic and prognostic evaluation of acute myocarditis remains still challenging. Particularly, acute atrial involvement could be underdiagnosed due to its limited evaluation by cardiac magnetic resonance (CMR) and the lack of sensitive basic echocardiographic indices.

Purpose
Our aim was to assess left atrial strain in a cohort of patients with diagnosis of acute myocarditis, and its correlation with incident cardiovascular events at follow up.

Methods
30 patients with acute myocarditis diagnosed by CMR, performed within one week from admission, according to Lake-Louise criteria were retrospectively included. Patients with poor acoustic window or missing data related to hospitalization or follow-up were excluded. Clinical characteristics, laboratory examinations, transthoracic echocardiography data were collected. Speckle tracking analysis was performed offline on the echocardiographic records. Follow up data were obtained via electronical records or phone-calls. Clinical endpoints were the development of all-cause or cardiovascular death, cardiovascular hospitalization (including heart failure, major arrhythmias, acute coronary syndromes), atrial fibrillation or ventricular arrhythmias onset.

Results
The study cohort, composed of 30 patients with acute myocarditis (mean age 38 ±15 years, 33% (n = 10) female), showed raised C-reactive protein and cardiac troponin at admission, beside a mild reduction of left ventricular ejection fraction (Fig.1). Left ventricular strain was preserved in the majority of patients (57%, n = 17) or mildly reduced, while left atrial strain was significantly reduced (Table 1). At CMR, 57% (n = 17) of patients presented myocardial edema and 70% (n = 21) presented late gadolinium enhancement. Over a mean follow up of 2.3 ± 1.9 years, 5 patients had hospitalizations for cardiovascular reasons, one of whom for heart failure, 3 patients developed atrial fibrillation, 5 patients developed ventricular arrhythmias. Patients with cardiovascular events showed lower left atrial strain than those without events (Fig.2); global atrial reservoir strain reached a statistically significant difference in patients with incident atrial fibrillation vs those without (p = 0.02).

Conclusions
our findings suggest that patients with acute myocarditis may have a subtle atrial involvement which could be detected by speckle tracking echocardiography. Moreover, lower values of left atrial strain may characterize patients at higher risk of incident atrial fibrillation during follow-up. Abstract Figure. Fig.1 Abstract Figure. Fig.2
Left ventricular apical aneurysm in Fabry disease: implications for clinical significance and risk stratification
Authors: Chang HC , Kuo L , Niu DM , Yu WC 
Published: February 2022

Abstract

Funding Acknowledgements
Type of funding sources: None.

Background
More than 50% of patients with Fabry disease (FD) present with cardiac phenotype, and left ventricular hypertrophy (LVH) is the most frequent manifestation. Among FD patients with LVH, a rare and novel presentation of left ventricular apical aneurysm (LVAA) has been reported.

Purpose
This study aimed to investigate the clinical incidence of LVAA and its impacts on prognosis among FD patients.

Methods
We retrospectively analyzed 268 FD patients at a tertiary medical center between January 2003 to September 2020. Two patients with ischemic LVAA were excluded. LVH and LVAA were confirmed either by echocardiography (Figure 1A) or cardiovascular magnetic resonance imaging (Figure 1B). The primary endpoints were a composite of heart failure (HF) hospitalization, sustained ventricular tachycardia (VT), stroke, and death.

Results
Of 266 FD patients, there were 105 (39.5%) patients had LVH, and 11 (10.3%) of them had LVAAs (age 67.5 ± 9.5 years, 8 males [72.7%]). After a mean follow-up of 49.3 ± 34.8 months, 8 patients experienced primary endpoints, including 5 (45.5%) HF hospitalizations, 3 (27.3%) VT, 1 (9.1%) stroke, and 4 (36.4%) deaths. The risk for composite adverse events was significantly higher in patients with LVAA compared with those without (8 [72.7%] vs 17 [18.1%]), leading to significantly lower event-free survival in patients with LVAA (Log-Rank P &lt; 0.001, Figure 2). The presence of LVAA was independently associated with an increased risk of composite adverse events (hazard ratio: 3.59; confidence interval: 1.30-9.91, P = 0.01) after adjusting for age, gender, advanced HF, renal function, dyslipidemia, atrial fibrillation, left ventricular ejection fraction of &lt;40%, average E/e’, and LV mass index.
Conclusions
LVAA presents in around 10% of patients with Fabry cardiomyopathy and is strongly associated with an increased risk of adverse events. The identification of this phenotype would be useful to identify high-risk patients with Fabry cardiomyopathy, among whom more aggressive treatments may be considered. Abstract Figure. Apical aneurysm in Fabry cardiomyopathy Abstract Figure. KM curve of composite adverse events
Machine-learning score using stress CMR for death prediction in patients with suspected or known CAD
Authors: Pezel T , Sanguineti F , Garot P , Unterseeh T , Champagne S , Toupin S , Morisset S , Hovasse T , Garot J 
Published: February 2022

Abstract

Funding Acknowledgements
Type of funding sources: None.

BACKGROUND
In patients with suspected or known coronary artery disease (CAD), traditional prognostic risk assessment is based upon a limited selection of clinical and imaging findings. Machine learning (ML) methods can take into account a greater number and complexity of variables.

PURPOSE
To investigate the feasibility and accuracy of ML using stress CMR and clinical data to predict 10-year all-cause mortality in patients with suspected or known CAD, and compared its performance to existing clinical or CMR scores.

METHODS
Between 2008 and 2018, a retrospective cohort study with a median follow-up of 6.0 years (interquartile range: 5.0-8.0) included all consecutive patients referred for stress CMR. Twenty-three clinical and 11 stress CMR parameters were evaluated. Machine learning involved automated feature selection by random survival forest, model building with a multiple fractional polynomial algorithm, and 5 repetitions of 10-fold stratified cross-validation. The primary outcome was all-cause death based on the electronic National Death Registry.

RESULTS
Of 31,752 consecutive patients (mean age 63.7 ± 12.1 years and 65.7% males), 2,679 (8.4%) died with 206,453 patient-years of follow-up. ML score (ranging 0 to 10 points) exhibited a higher area-under-the-curve compared with C-CMR-10-score, ESC-score, QRISK3-score, FRS and stress CMR data alone for prediction of 10-year all-cause mortality (ML: 0.76 vs. C-CMR-10-score: 0.68, ESC-score: 0.66, QRISK3-score: 0.64, FRS: 0.63, extent of inducible ischemia: 0.66, extent of LGE: 0.65, all p &lt; 0.001).

CONCLUSIONS
The ML score including clinical and stress CMR data exhibited a higher prognostic value to predict 10-year death compared with all traditional clinical or CMR scores. Abstract Figure. Random survival Forest: ML score Abstract Figure. Prognostic Value of ML score
Multimodality imaging in the evaluation of left ventricle myocardial deformation determined by edema and scar in acute myocarditis
Authors: Angelini G , Bonanni M , Paiocchi VL , Leo LA , Schlossbauer S , Pasotti E , Pedrazzini G , Valgimigli M , Favale S , Forleo C , Pavon AG , Faletra F 
Published: February 2022

Abstract

Funding Acknowledgements
Type of funding sources: None.

INTRODUCTION
acute myocarditis (aMY) is characterized by the presence of edema and myocardial scar detected by cardiovascular magnetic resonance (CMR).
Aim of our study is to investigate the diagnostic value of two-dimensional (2D) speckle tracking echocardiography and Cardiovascular Magnetic Resonance (CMR) feature tracking (FT) in detecting edema and myocardial scar in aMY.

METHODS
all consecutive patients with clinically suspected aMY were enrolled in our study. Inclusion criteria were: 1) new ECG abnormalities 2) myocardial cytolysis markers and 3) absence of angiographically detectable coronary artery disease. Exclusion criteria included poor cine image quality caused by respiratory motion and arrhythmia. All patients underwent transthoracic echocardiography and cardiac function was evaluated by a comprehensive assessment of LV function, including 2D speckle-tracking echocardiography (2D STE). CMR was performed in all patients in a 3T scanner. Extension of edema and myocardial scar were respectively evaluated on T2 mapping and on Late Gadolinium Enhancement sequences considering numbers of segments involved according to 17-segment model (AHA). FT analysis of the left ventricle (LV) was performed using the Tissue Tracking Module to obtain LV strain data.

RESULTS
52 patients were included in the study, mean age was 36± 17 years, three patients were female (6%). Mean LVEF was 56,2 ± 7,2 % and mean end diastolic volume index (EDVi) was 62,52 ± 19,02 ml/m2. 10 patients (19,2%) had impaired EF with mean values of 44,6 ± 6,15%. aMY was confirmed in all patients with the presence of myocardial edema and subepicardial late gadolinium enhancement (LGE). Inferior segments were involved in 28 patients (53,8%), lateral segment in 19 patients (36,5%), septal segments in 7 patients (13,5%) and anterior segments in 15 patients (28,8%). 2D STE LV GLS was -16.41± 5,47% while CMR-FT LV GLS was -19,07 ± 4,65%, showing a good agreement between the two methods (r = 0,5; p &lt; 0,001). The univariate analysis showed a significant correlation between 2D STE LV GLS and CMR-FT LV GLS with the extension of myocardial edema assessed by CMR (r= 0,43; p= 0,002 and r = 0.47; p = 0,002 respectively). The univariate analysis did not show a significant correlation between 2D STE LV and the extension of myocardial scar assessed by CMR (r= 0.2; p= 0.155) while a significant correlation was found between CMR-FT LV GLS and myocardial scar (r = 0.4; p = 0.01).

CONCLUSIONS
in patients with aMY, good correlation was found between CMR-FT and 2D STE in the evaluation of GLS. 2D STE LV GLS and CMR-FT LV GLS proved to have a good correlation with the extension of myocardial edema, while only CMR-FT LV GLS proved to be associated with myocardial scar extension.
Vessel-specific plaque features on coronary computed tomography angiography among patients of varying atherosclerotic cardiovascular disease risk
Authors: Bax A Maxim , Yoon Yeonyee E , Gianni Umberto , van Rosendael Alexander R , Lu Yao , Ma Xiaoyue , Goebel Benjamin P , Tantawy Sara W , Andreini Daniele , Budoff Matthew J , Cademartiri Filippo , Chinnaiyan Kavitha , Choi Jung Hyun , Conte Edoardo , de Araújo Gonçalves Pedro , Gottlieb Ilan , Hadamitzky Martin , Leipsic Jonathon A , Maffei Erica , Pontone Gianluca , Shin Sanghoon , Kim Yong Jin , Lee Byoung Kwon , Chun Eun Ju , Sung Ji Min , Lee Sang Eun , Berman Daniel S , Narula Jagat , Lin Fay Y , Chang Hyuk Jae , Shaw Leslee J ,   for the PARADIGM investigators
Published: February 2022

Abstract

Aims
The relationship between AtheroSclerotic CardioVascular Disease (ASCVD) risk and vessel-specific plaque evaluation using coronary computed tomography angiography (CCTA), focusing on plaque extent and composition, has not been examined. To evaluate differences in quantified plaque characteristics (using CCTA) between the three major coronary arteries [left anterior descending (LAD), right coronary (RCA), and left circumflex (LCx)] among subgroups of patients with varying ASCVD risk.

Methods and results
Patients were included from a prospective, international registry of consecutive patients who underwent CCTA for evaluation of coronary artery disease. ASCVD risk groups were &lt;7.5% (low), 7.5–20% (intermediate), and ≥20% (high). Among the ASCVD risk groups, the three coronary arteries were compared regarding quantified plaque volume and composition. Whole-heart plaque quantification was performed in 1340 patients (age 60 ± 9 years, 58% men). Across low, intermediate, and high ASCVD risk patients, the volume of plaque increased proportionally but was least in the LCx (7.4, 9.0, and 25.3 mm3, respectively) as compared with the RCA (19.3, 32.6, and 67.0 mm3, respectively, all P ≤ 0.006) and LAD (39.9, 60.8, and 93.3 mm3, respectively, all P &lt; 0.001). In each ASCVD risk group, the composition of plaque in the LCx exhibited the least necrotic core and fibrofatty plaque (P &lt; 0.05 vs. LAD and RCA).

Conclusion
Among patients with varying risk of ASCVD, plaque in the LCx is decidedly less and is comprised of less non-calcified plaque supporting prior evidence of the lower rates of acute coronary events in this vessel.
Pool-GWAS on reproductive dormancy in Drosophila simulans suggests a polygenic architecture
Authors: Lirakis Manolis , Nolte Viola , Schlötterer Christian 
Published: February 2022

Abstract
The genetic basis of adaptation to different environments has been of long-standing interest to evolutionary biologists. Dormancy is a well-studied adaptation to facilitate overwintering. In Drosophila melanogaster, a moderate number of genes with large effects have been described, which suggests a simple genetic basis of dormancy. On the other hand, genome-wide scans for dormancy suggest a polygenic architecture in insects. In D. melanogaster, the analysis of the genetic architecture of dormancy is complicated by the presence of cosmopolitan inversions. Here, we performed a genome-wide scan to characterize the genetic basis of this ecologically extremely important trait in the sibling species of D. melanogaster, D. simulans that lacks cosmopolitan inversions. We performed Pool-GWAS in a South African D. simulans population for dormancy incidence at 2 temperature regimes (10 and 12°C, LD 10:14). We identified several genes with SNPs that showed a significant association with dormancy (P-value &lt; 1e-13), but the overall modest response suggests that dormancy is a polygenic trait with many loci of small effect. Our results shed light on controversies on reproductive dormancy in Drosophila and have important implications for the characterization of the genetic basis of this trait.
A chromosomal-level reference genome of the widely utilized Coccidioides posadasii laboratory strain “Silveira”
Authors: de Melo Teixeira Marcus , Stajich Jason E , Sahl Jason W , Thompson George R , Brem Rachel B , Dubin Claire A , Blackmon Austin V , Mead Heather L , Keim Paul , Barker Bridget M 
Published: February 2022

Abstract
Coccidioidomycosis is a common fungal disease that is endemic to arid and semi-arid regions of both American continents. Coccidioides immitis and Coccidioides posadasii are the etiological agents of the disease, also known as Valley Fever. For several decades, the C. posadasii strain Silveira has been used widely in vaccine studies, is the source strain for production of diagnostic antigens, and is a widely used experimental strain for functional studies. In 2009, the genome was sequenced using Sanger sequencing technology, and a draft assembly and annotation were made available. In this study, the genome of the Silveira strain was sequenced using single molecule real-time sequencing PacBio technology, assembled into chromosomal-level contigs, genotyped, and the genome was reannotated using sophisticated and curated in silico tools. This high-quality genome sequencing effort has improved our understanding of chromosomal structure, gene set annotation, and lays the groundwork for identification of structural variants (e.g. transversions, translocations, and copy number variants), assessment of gene gain and loss, and comparison of transposable elements in future phylogenetic and population genomics studies.
Effects of harvest date and growth stage on triticale forages in the southwest USA: kinetics of in vitro disappearance of fiber and dry matter
Authors: Coblentz Wayne K , Ottman Michael J 
Published: February 2022

AbstractRecently, there has been interest in including triticale (X Triticosecale Wittmack) within forage programs in the southwest USA. Our objectives were to evaluate in vitro disappearance kinetics of neutral detergent fiber (NDF) and dry matter (DM) for cultivars identified during 2019 as positively or negatively deviant from typical cultivars, based specifically on regressions of 48-h in vitro disappearance of NDF on growth stage (GRST). All NDF analyses included the use of heat-stable α-amylase and sodium sulfite, as well as correction for residual ash (asNDFom). Seven triticale cultivars were established on December 18, 2019 at the University of Arizona Maricopa Agricultural Center, located near Maricopa, AZ. Forage plots were arranged in a randomized complete block design with three complete blocks (replications), and then harvested on seven dates the following late-winter and spring (February 26, March 17, April 1, April 14, April 28, May 12, and May 26). Based on a linear model, GRST was highly variable among cultivars on March 17 (44 ± 10.6), April 1 (57 ± 12.1), April 14 (67 ± 8.9), and April 28 (79 ± 7.2) compared with other harvest dates (SD ≤ 1.7). For concentrations of asNDFom, all cultivars exhibited linear (P ≤ 0.042) and quadratic (P &lt; 0.001) polynomial contrasts in response to harvest date, and all cultivars except Merlin Max (P ≥ 0.063) exhibited at least one additional cubic or quartic effect (P ≤ 0.015). A contributing factor to the unique response by Merlin Max was the numerically greater maximum canopy height (145 ± 9.8 cm) compared with the mean of all cultivars (107 ± 17.7 cm), which also was associated with greater percentages of stem, as well as reduced percentages of DM partitioned within the grain head. Regressions of asNDFom disappearance after 30- or 48-h incubations on GRST indicated this was an effective independent variable (R2 ≥ 0.927), and responses were most often linear in nature. Generally, relationships for DM disappearance were quadratic, ostensibly due to the complicating effect of grain fill, but GRST was again an effective predictor variable with R2 statistics ≥ 0.852 for 12 of 14 combinations of cultivar and incubation time. Predicted percentages of digestible DM attributed to asNDFom disappearance were ≥50.3% through the fully flowered stage of growth, but digestible contributions from nonfiber components following the onset of grain fill profoundly affected overall DM digestibility among cultivars harvested at later GRST.