bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of a Novel Naphtiridine‐based Covalent Triazine Framework for Carbon Dioxide Capture and Storage Applications
Authors: Tuci Giulia , Pugliesi Matteo , Rossin Andrea , Pham‐Huu Cuong , Berretti Enrico , Giambastiani Giuliano 
Published: November 2022

AbstractCovalent triazine frameworks (CTFs) represent a sub‐class of Porous Organic Polymers showing high chemical/thermal stability, large permanent porosity and intrinsically high N‐content. All these features make them ideal candidates for Carbon Capture and Storage (CCS) applications. Herein, a novel CTF has been prepared under ionothermal conditions through cyclotrimerization of a newly designed dicyano‐building block with high N/C ratio (2,6‐Dicyano‐1,5‐naphthyridine, 4). The as prepared CTF‐Napht showed remarkable carbon dioxide uptake at ambient conditions, with a loading of 3.93 mmol/g (17.3 wt. %) at 1 bar and 298 K that outperforms many benchmark CTF systems from the literature. The ad‐hoc designed dicyano building unit together with a judicious choice of synthetic conditions have imparted to CTF‐Napht an outstanding affinity towards CO2 confirmed by its isosteric heat of adsorption (Qst) as high as 39.6 KJ/mol that ranks among the highest reported so far for CTF‐based polymers.
Efficient Pathogen Capture and Sensing Promoted by Dynamic Deformable Nanointerfaces
Authors: Cao Ying , Wu Na , Li Hui‐Da , Xue Jing‐Wen , Wang Rui , Yang Ting , Wang Jian‐Hua 
Published: November 2022

AbstractThe M13 bacteriophage (M13 phage) has emerged as an attractive bionanomaterial due to its chemistry/gene modifiable feature and unique structures. Herein, a dynamic deformable nanointerface is fabricated taking advantage of the unique feature of the M13 phage for ultrasensitive detection of pathogens. PIII proteins at the tip of the M13 phage are genetically modified to display 6His peptide for site‐specific anchoring onto Ni‐NTA microbeads, whereas pVIII proteins along the side of the M13 phage are orderly arranged with thousands of aptamers and their complementary strands (c‐apt). The flexible M13 nanofibers with rich recognition sites act as octopus tentacles, resulting in a 19‐fold improvement in the capture affinity toward the target. The competitive binding of the target pathogen releases c‐apts and initiates rolling circle amplification (RCA). The sway motion of M13 nanofibers accelerates the diffusion of c‐apts, thus promoting RCA efficiency. Benefiting from the strengthened capture ability toward the target and the accelerated RCA process, three‐orders of magnitude improvement in the sensitivity is achieved, with a detection limit of 8 cfu mL−1 for Staphylococcus aureus. The promoted capture ability and assay performance highlights the essential role of the deformable feature of the engineered interface. This may provide inspiration for the construction of more efficient reaction interfaces.
Constructing Janus Microsphere Membranes for Particulate Matter Filtration, Directional Water Vapor Transfer, and High‐Efficiency Broad‐Spectrum Sterilization
Authors: Deng Tong , Chen Yifu , Liu Yanbo , Shang Zeren , Gong Junbo 
Published: November 2022

AbstractCommercial masks have significant drawbacks, including low water vapor transmission efficiency and limited ability to inhibit harmful microorganisms, whereas in this contribution, a series of Janus microsphere membranes are developed with hierarchical structures by quenching and crystallizing 12‐hydroxystearic acid and halicin layer‐by‐layer on a polypropylene non‐woven fabric, laminating them with hydrophilic cotton fibers in a one‐pot process, and further demonstrate the potential of this composite system as masks. Through further optimization, excellent superhydrophobic/superhydrophilic properties (contact angle 157.1°/0°), superior filtering effects (93.54% for PM2.5 and 98.35% for PM10), with a low‐filtration resistance (57 Pa) and a quality factor of up to 0.072 Pa−1 are achieved, all better than that of commercial N95 masks. In addition, the membrane allows for the directional transport of water vapor from the inside out, increasing the water vapor transmission rate by more than 20% compared with the monolayer hydrophobic microsphere membrane. It also has a bactericidal capacity of over 99.9999% against Escherichia coli and is tested for robustness and stability in various extreme environments. This work may shed light on designing novel filter media with versatile functions, meanwhile, the materials can also be used in protective equipment against the new coronavirus.
Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip
Authors: Kalyana Sundaram Ramalingam Venkat , Bera Manindra , Coleman Jeff , Weerakkody Jonathan S. , Krishnakumar Shyam S. , Ramakrishnan Sathish 
Published: November 2022

AbstractCellular plasma membranes, in their role as gatekeepers to the external environment, host numerous protein assemblies and lipid domains that manage the movement of molecules into and out of cells, regulate electric potential, and direct cell signaling. The ability to investigate these roles on the bilayer at a single‐molecule level in a controlled, in vitro environment while preserving lipid and protein architectures will provide deeper insights into how the plasma membrane works. A tunable silicon microarray platform that supports stable, planar, and asymmetric suspended lipid membranes (SLIM) using synthetic and native plasma membrane vesicles for single‐molecule fluorescence investigations is developed. Essentially, a “plasma membrane‐on‐a‐chip” system that preserves lipid asymmetry and protein orientation is created. By harnessing the combined potential of this platform with total internal reflection fluorescence (TIRF) microscopy, the authors are able to visualize protein complexes with single‐molecule precision. This technology has widespread applications in biological processes that happen at the cellular membranes and will further the knowledge of lipid and protein assemblies.
A validated square‐wave voltammetric method for simultaneous determination of two insecticide metabolites: 3,5,6‐trichloro‐2‐pyridinol and malathion diacid and their binding constants with human serum albumin
Authors: Abo El‐Maali Nagwa , Shafea Naglaa Sayed , Mahmoud Hamada Mohamed , Nassar Hossam Fathy 
Published: November 2022

AbstractA proposed validated square‐wave voltammetric method and high‐performance liquid chromatography, as a comparative method, are applied to measure simultaneously two metabolites‐ of current use pesticides‐ namely 3,5,6‐trichloro‐2‐pyridinol and malathion dicarboxylic acid. Method validation and quality control parameters are studied. Method detection limits are found to be 0.043 and 0.061 μg/ml for 3,5,6‐trichloro‐2‐pyridinol and malathion dicarboxylic acid, respectively whereas the practical quantification limits are found to be 0.135 and 0.193 μg/ml (voltammetry), 0.05 and 0.1 μg/ml (high‐performance liquid chromatography) for 3,5,6‐trichloro‐2‐pyridinol and malathion dicarboxylic acid, respectively. The application of the proposed method for the analysis of water samples collected from different sources has been assessed. The interaction of these metabolites with human serum albumin was studied, a 1:1 association complex was formed and their binding constants were evaluated through the calculation to be 0.30 × 102/M for 3,5,6‐trichloro‐2‐pyridinol and 8.414 × 103/M for malathion dicarboxylic acid. The low value of the binding of 3,5,6‐trichloro‐2‐pyridinol metabolite indicates that this metabolite is more likely to be more toxic than the other metabolite.
Validity aspects in measuring evolution acceptance: Evidence from surveys of preservice biology teachers and creationists
Authors: Beniermann Anna , Moormann Alexandra , Fiedler Daniela 
Published: November 2022

AbstractOver the past decades, a large body of research has examined students' magnitudes of evolution acceptance and related measurement issues resulting in questions concerning instruments' validity and operationalization. Until now, several studies investigated validity aspects of often‐used evolution acceptance instruments and came to diverging conclusions about instruments' scores comparability. Within the last years, religious identity was identified as a significant predictor for magnitudes of evolution acceptance. However, religious identity can also point to validity issues if aspects of the content under investigation are interpreted differently based on specific religious identity. Additionally, self‐identified creationists could serve as a source of validity evidence due to the assumption that creationists should score lower on an evolution acceptance instrument than groups with more scientifically adequate views. Thus, we aim to provide additional validity evidence for often‐used evolution acceptance instruments within a European context (i.e., Germany) by comparing two groups of particular interest for research in science education: preservice biology teachers and self‐identified creationists. Exploring evidence based on (1) internal structure, (2) relationships with other variables, and (3) test content provides arguments for test interpretations' validity. A total of 286 persons (206 preservice biology teachers and 81 self‐identified creationists) participated in a survey comprised of six often‐used evolution acceptance measures (i.e., MATE, GAENE, I‐SEA, ATEVO, Gallup question, and 100 point questions). Overall, our findings indicate that the six applied instruments demonstrate differences concerning their evidence for sound interpretation. In particular, the DIF and dimensionality analyses lead to the assumption that the GAENE may be multidimensional other than theoretically expected. In general, the multi‐item measures were stronger related to each other than to the general public polls. Using an instrument that differentiates between microevolution and macroevolution is advisable for creationist samples or samples with a remarkable share of creationists' views.
The role and mechanism of unfolded protein response signaling pathway in methylmercury‐induced apoptosis of mouse spermatocytes germ cell‐2 cells
Authors: Zhang Xiayu , Hao Huifang , Ma Kai , Pang Huan , Li Xinyue , Tian Tiantian , Hou Shanshan , Ning Xiaofan , Wu Hao , Hou Qiaohong , Li Meng , Sun Yunxiang , Song Xiuling , Jin Minghua 
Published: November 2022

AbstractThe study aimed to explore the role and mechanism of unfolded protein response (UPR) in methylmercury (MeHg)‐induced Mouse Spermatocytes (GC‐2spd[ts]) apoptosis. Methods such as MTT, flow cytometry, and Western Blot were used to evaluate the cell viability, membrane potential (MMP), reactive oxygen species (ROS), calcium ion (Ca2+), rate of cell apoptosis, and the expression of apoptosis‐related and UPR‐related protein. The results showed that with the increase of MeHg concentration, cell viability and MMP decreased, ROS, Ca2+, rate of cell apoptosis, and the expression of apoptosis‐related protein and UPR‐related protein increased. To further explore the effect of ROS‐induced oxidative damage on it, the ROS inhibitor N‐acetyl‐L‐cysteine (NAC) was used. The effects of MeHg on germ cell (GC‐2) cells were partially inhibited after NAC pretreatment. Our present study proved that MeHg might induce cell apoptosis by activating the UPR signaling pathway in GC‐2 cells and affect normal reproductive function.
Leaking the IPCC: A question of responsibility?
Authors: Hartz Friederike 
Published: November 2022

AbstractIn August 2021, while the world was grappling with the release of the IPCC WGI report, a group of activist scientists called Scientist Rebellion leaked parts of the Working Group III contribution to the Sixth Assessment Report (AR6) prior to intergovernmental approval. Although Scientist Rebellion are not the first to leak an IPCC report, they are the most vocal leaker with a particular political agenda: to generate disruptive climate action by curtailing the carefully orchestrated intergovernmental process of the IPCC. I take this case of science in activism involving the IPCC interface as an example to examine the increasingly intricate relationship between science, activism and responsibility. The salient sense of urgency around climate action, the growing prevalence of the climate crisis narrative in both public and scientific spheres, and the increased policy‐relevance of science‐policy interfaces put strong pressures on (climate) scientists that need to be disentangled to be understood without premature judgment. I show that the leak is symptomatic of the novel responsibility to act(ivism) scientists are increasingly confronted with and I highlight some of the tensions that come with this responsibility. Emphasizing the centrality of the question of responsibility in, and of, science, I discuss the (ir)responsibility of leaking IPCC draft materials. I end on a call for more interdisciplinary attentiveness to the nexus of science, activism and responsibility and the cases in which they become entangled.This article is categorized under:
The Social Status of Climate Change Knowledge > Climate Science and Social Movements
The Social Status of Climate Change Knowledge > Knowledge and Practice
The Social Status of Climate Change Knowledge > Climate Science and Decision Making
Grease in the Nucleus: Insights into the Dynamic Life of Nuclear Membranes
Authors: Anand Deepak , Chaudhuri Arunima 
Published: November 2022

AbstractNucleus is at the center stage of cellular drama orchestrated in the life of a cell and the nucleoplasm is surrounded by a double membranous compartment constituting the Nuclear membrane/envelope (NE) that separates it from the cytoplasm in nucleated cells. The initial understanding of the NE was that of a border security entity between the nucleus and the cytoplasm, separating gene regulation and transcription in the nucleus from translation in the cytoplasm. However, the discovery of a wide array of inherited diseases caused by mutations in genes encoding proteins that reside or interact with NE diverted the interest into deciphering the lipid-protein-rich environment of the NE. Today, the NE is considered a dynamic organelle which forms a functional linkage between the nucleus and the rest of the cell. The exposure of NE to constant mechanical constraints by its connectivity to the large polymer network of the lamina and chromatin on one side, and to the cytoskeleton on the other side results, in a variety of shape changes. We discuss two such deformation, the formation of nuclear blebs and nucleoplasmic reticulum (NER). Although the protein and the lipid composition of NE comprises a small fraction of the total lipid-protein load of the cell, the ability to define the lipid-protein composition of Inner nuclear membrane (INM) and Outer nuclear membrane (ONM) with precision is crucial for obtaining a deeper mechanistic understanding of their lipid-protein interaction and the various signaling pathways that are triggered by them. In addition, this allows us to further understand the direct and indirect roles of NE machinery in the chromosomal organization and gene regulation.
Graphical Abstract
Swallowing-related quality of life in children with oesophageal atresia: a national cohort study
Authors: Bergmann Sandra , Ritz Laura Antonia , Widenmann-Grolig Anke , Jechalke Stephan , von Schweinitz Dietrich , Hubertus Jochen , Lurz Eberhard 
Published: November 2022

AbstractSwallowing and feeding disorders are a major concern for children with oesophageal atresia (OA) after primary or staged OA repair. Primary OA repair is associated with higher rates of short-term complications in preterm infants with very low birth weight (VLBW) or extreme low birth weight (ELBW). On the other hand, primary repair may have the benefit of early commencement of oral feedings. We hypothesize that also in the medium-term, swallowing-related quality of life is better after primary oesophageal repair. We conducted a prospective cross-sectional study on swallowing quality in a national cohort of former VLBW and ELBW children with OA, using the structured paediatric swallowing quality of life (pedSWAL-QOL) questionnaire. Results were correlated with surgical approach and baseline clinical data. Principal component analysis of pedSWAL-QOL domains was performed. In total, 44 complete data sets of 78 children were available. The mean age of children was 8.5 years (SD = 7.4), and 23 children (52%) had primary OA repair. The overall median pedSWAL-QOL score was 2 (IQR = 0–3), representing a high swallowing-related quality of life, independent of surgical technique (p = 0.086). Children with a history of intracranial haemorrhage (ICH) (p = 0.002) and those with VACTERL association (p = 0.008) had significantly decreased enjoyment with eating. In addition, children with VACTERL association had problems to find suitable foods (p = 0.04). Conclusion: In this national cohort of VLBW and ELBW preterm-born children with OA, swallowing-related quality of life is good, mostly independent of initial surgery. Children with OA and ICH or VACTERL association may require more intense support with feeding.

What is Known:• Dysphagia, resembling feeding and swallowing disorders, is common in children and adults with repaired oesophageal atresia. Nevertheless, dysphagia in children with oesophageal atresia decreases with age.• Parents of younger children suffer from increased anxiety and fear regarding eating and swallowing abilities of their children.

What is New:• Swallowing-related quality of life in former preterm children with oesophageal atresia is good, independent of initial surgical approach (primary vs. staged repair), even in very low birth weight or extreme low birth weight infants. • Children suffering from VACTERL association or intracranial haemorrhage show decreased enjoyment with eating.
What makes a student feel vital? Links between teacher-student relatedness and teachers’ engaging messages
Authors: Santana-Monagas Elisa , Núñez Juan L. , Loro Juan F. , Moreno-Murcia Juan A. , León Jaime 
Published: November 2022

AbstractRecent studies suggest that teacher messages can affect students’ well-being. Using a multilevel, variable, and person-centred approach, this study aimed to identify profiles of students according to their teachers’ use of engaging messages and analyse the relation among these profiles and teacher-student relatedness and students’ subjective vitality. A total of 1209 students participated in the study. At the student-level, profile analysis indicated the existence of four different profiles: the few messages profile, the autonomous motivational appeals profile, the loss-framed messages profile, and the gain-framed messages profile. At the teacher level, profile analysis indicated the existence of two profiles: the variant and the invariant profiles. Results showed that overall, at both levels of analysis, teachers’ engaging messages related with teacher-student relatedness (either positively or negatively) with clear differences among profiles. Moreover, also at both levels of analysis, teacher-student relatedness related with students’ subjective vitality. Main findings and implications for practice are discussed.
Numerical study of opposed zero-net-mass-flow jet-induced erythrocyte mechanoporation
Authors: Liu Xinyue , Ai Jinfang , Xie Jun , Hu Guohui 
Published: November 2022

AbstractWith the advantages of biosafety and efficiency, increasing attention has been paid to the devices for gene and macromolecular drug delivery based on mechanoporation. The transient pore formation on the cell membrane allows cargo transportation when the membrane areal strain is beyond the critical pore value and below the lysis tension threshold. Based on this principle, we propose a method to apply the proper fluid stress on cells moving in a microchannel under the action of zero-net-mass-flux (ZNMF) jets. In this study, an immersed finite element method (IFEM) is adopted to simulate the interaction between the cells and the fluid fields so as to investigate the cell movement and deformation in this mechanoporation system. To evaluate the efficiency of the cargo delivery, a pore integral is defined as the mean pore rate when the cell passes through the jet region. By analyzing the effects of the parameters, including the pressure gradient along the microchannel, the jet amplitude, and the jet frequency, on the pore integrals, a group of optimized parameters for cargo delivery efficiency are obtained. Additionally, the stability and safety of this system are analyzed in detail. These results are helpful in designing the mechanoporation devices and improving their efficiency of drug delivery.
Causal Influences of Same-Sex Attraction on Psychological Distress and Risky Sexual Behaviors: Evidence for Bidirectional Effects
Authors: Oginni Olakunle Ayokunmi , Lim Kai Xiang , Purves Kirstin Lee , Lu Yi , Johansson Ada , Jern Patrick , Rijsdijk Frühling Vesta 
Published: November 2022

AbstractAlthough health disparities among same-sex attracted compared to heterosexual individuals are typically explained by minority stress, there is limited evidence for a causal effect. This study investigated whether same-sex attraction was causally associated with psychological distress and risky sexual behavior using sociosexual behavior as a proxy. The sample comprised monozygotic and dizygotic twins and their non-twin siblings (n = 2036, 3780 and 2356, respectively) genotyped and assessed for same-sex attraction, psychological distress (anxiety and depressive symptoms), and risky sexual behavior. Causal influences were investigated with same-sex attraction as the predictor and psychological distress and risky sexual behavior as the outcomes in two separate Mendelian Randomization-Direction of Causation (MRDoC) models using OpenMx in R. The MRDoC model improves on the Mendelian Randomization and Direction of Causation twin models by allowing analyses of variables with similar genetic architectures, incorporating polygenic scores as instrumental variables and specifying pleiotropy and residual covariance. There were significant causal influences flowing from same-sex attraction to psychological distress and risky sexual behavior (standardized coefficients = 0.13 and 0.16; 95% CIs 0.03–0.23 and 0.08–0.25, respectively). Further analyses also demonstrated causal influences flowing from psychological distress and risky sexual behavior toward same-sex attraction. Causal influences from same-sex attraction to psychological distress and risky sexual behavior may reflect minority stress, which reinforces ongoing measures to minimize social disparities. Causal influences flowing in the opposite direction may reflect rejection sensitivity, stigma-inducing outcomes of risky sexual behavior, and recall bias; however, further research is required to specifically investigate these processes.