bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics of Microbiota in Three Backslopped Liquid Sourdoughs That Were Triggered with the Same Starter Strains
Authors: Tolu Valentina , Fraumene Cristina , Carboni Angela , Loddo Antonio , Sanna Manuela , Fois Simonetta , Roggio Tonina , Catzeddu Pasquale 
Published: October 2022

The preparation of sourdough may include the use of starter microorganisms to address the fermentation process toward specific conditions. The aim of this work was to study the dynamics of the microbial ecosystem in three liquid sourdoughs (SD1, SD2 and SD3) triggered with the same microbial strains. Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum), Saccharomyces cerevisiae and Candida lambica strains were inoculated as starters, and sourdoughs were differentiated for the fermentation conditions and for the method of starter inoculation. The analyses were performed on the three sourdoughs propagated in the laboratory for 22 days and on the sample SD1, which was transferred to a bakery and refreshed over many months. The dynamics of microbial communities were studied by plate-count analysis and metataxonomic approach. The acidity of sourdough was evaluated over time. Metataxonomic analysis highlighted a large heterogeneity of fungi microbiota in all sourdough preparations, many of them probably originated from the flour, being pathogens of plants. Few yeast species were found, and S. cerevisiae was plentiful but did not predominate over the other species, whereas the C. lambica species decreased over time and then disappeared in all preparations. The bacterial microbiota was less heterogeneous than the fungi microbiota; the species L. plantarum, Leuconostoc citreum and Levilactobacillus brevis (formerly known as Lactobacillus brevis) were always present in all sourdoughs, whereas Fructilactobacillus sanfranciscensis (formerly known as Lactobacillus sanfranciscensis) became the dominant species in bakery-propagated SD1 and in SD2 at the end of the propagation period.
Gene Regulatory Network of the Noncoding RNA Qrr5 Involved in the Cytotoxicity of Vibrio parahaemolyticus during Infection
Authors: Zha Fei , Pang Rui , Huang Shixuan , Zhang Jumei , Wang Juan , Chen Moutong , Xue Liang , Ye Qinghua , Wu Shi , Yang Meiyan , Gu Qihui , Ding Yu , Zhang Hao , Wu Qingping 
Published: October 2022

Small non-coding RNAs (sRNAs) in bacteria are important regulatory molecules for controlling virulence. In Vibrio spp., Qrr sRNAs are critical for quorum-sensing pathways and regulating the release of some virulence factors. However, the detailed role of Qrr sRNAs in the virulence of Vibrio parahaemolyticus remains poorly understood. In this study, we identified a Vibrio sRNA Qrr5 that positively regulates cytotoxicity and adherence in Caco-2 cells by primarily regulating the T3SS1 gene cluster. A number of 185, 586, 355, and 74 differentially expressed genes (DEGs) detected at 0, 2, 4, and 6 h post-infection, respectively, were mainly associated with ABC transporters and two-component system pathways. The DEGs exhibited a dynamic change in expression at various time points post-infection owing to the deletion of Qrr5. Accordingly, 17 related genes were identified in the co-expression network, and their interaction with Qrr5 was determined based on weighted co-expression network analysis during infection. Taken together, our results provide a comprehensive transcriptome profile of V. parahaemolyticus during infection in Caco-2 cells.
An Overview of Genus Malachra L.—Ethnobotany, Phytochemistry, and Pharmacological Activity
Authors: Cervantes-Ceballos Leonor , Sánchez-Hoyos Jorge , Sanchez-Hoyos Fredys , Torres-Niño Erick , Mercado-Camargo Jairo , Echeverry-Gómez Amparo , Jotty Arroyo Karick , del Olmo-Fernández Esther , Gómez-Estrada Harold 
Published: October 2022

The genus Malachra L. belongs to the family Malvaceae. It includes herbs or subshrubs of nine accepted species with approximately thirty synonyms, and it has been widely used in community folk medicine to treat health problems including inflammation, nasal obstruction, leishmaniasis, malaria, childbirth, kidney disorders, fever, respiratory tract diseases, among others. From the genus Malachra L., flavonoids, steroids, triterpenes, anthocyanins, leucoanthocyanins, saponins, carbohydrates, phenols, glycosides, and alkaloids have been isolated and identified. Some pharmacological reports have indicated that the genus has antidiarrheal, antiepileptic, antiulcerogenic, antioxidant, anticonvulsant, antiviral, anticancer, antibacterial, anthelmintic, and hepatoprotective properties. However, there have been limited studies of bioactive molecules with pharmacological and biological activities associated with Malachra alceifolia Jacq., Malachra capitata (L.) L., Malachra fasciata Jacq., Malachra radiata (L.) L., Malachra ruderalis Gürke., Malachra rudis Benth., Malachra helodes Mart., Malachra urens Poit. ex Ledeb. & Alderstam., and Malachra officinalis Klotzsch. In this review, we consider the conservation of these species to save the ancestral knowledge of their traditional use in populations, and their pharmacological potential for future studies in search of alternatives for solutions to diseases in humans and animals and tools for the design and search of potential bioactive compounds against infectious and non-infectious agents.
Chemical Composition and Cytotoxic Activity of Extracts from Carpesium divaricatum: In Vitro- versus Field-Grown Plants
Authors: Malarz Janusz , Galanty Agnieszka , Stojakowska Anna 
Published: October 2022

Carpesium divaricatum Sieb. & Zucc. is a plant species rich in terpenoids of anti-inflammatory and cytotoxic activity, especially germacranolides of potential medicinal value. The present study describes in vitro multiplication of C. divaricatum, analysis of active constituents in the multiple shoots, and assessment of cytotoxic activities of extracts prepared from in vitro- and field-grown plants. The plant extracts were evaluated for cytotoxicity using two melanoma cell lines (HTB140 and A375); human keratinocytes (HaCaT); two colon cancer cell lines (Caco2 and HT29); human hepatocellular carcinoma cells (HepG2); two lines of prostate cancer cells (DU145 and PC3) and prostate epithelial cells (PNT2). Chemical compositions of the assayed extracts were analyzed by HPLC/DAD, in reference to isolated compounds. Maximum of 4.07 ± 1.61 shoots regenerated from a nodal explant of C. divaricatum, cultivated in a liquid MS medium supplemented with thidiazuron (1 μM). In vitro grown shoots and plantlets of C. divaricatum accumulated terpenoids that are known as active constituents of the intact plant. Cytotoxic activity of the extracts prepared from the in vitro cultured plants was like that demonstrated by the extracts prepared from field-grown plants and seemed to be more selective than cytotoxicities of the individual germacranolides.
Pertumbuhan Ekonomi Sumatra Barat Ditinjau dari Pendapatan Asli Daerah dan Dana Alokasi Umum dengan belanja Daerah sebagai Pemediasi
Authors: Rhamedia Hartika 
Published: October 2022

Penelitian ini bertujuan untuk mengetahui pengaruh Pendapatan Asli Daerah, Dana Alokasi Umum terhadap Pertumbuhan Ekonomi dengan Belanja Daerah sebagai variabel intervening di pemerintahan kabupaten / kota Provinsi Sumatera Barat. Jenis data yang dikumpulkan merupakan data sekunder yang diperoleh dari website BPS. Jumlah sampel yang digunakan sebanyak 95 dari daerah Kabupaten/Kota di Provinsi Sumatera Barat. Metode analisis data dan pengujian hipotesis menggunakan analisis statistik deskriptif, analisis regresi jalur. Hasil penelitian ini menunjukkan bahwa Pendapatan Asli Daerah (PAD), berpengaruh signifikan terhadap Belanja Daerah (BD) pada Provinsi Sumatera Barat. Dana Alokasi Umum (DAU), berpengaruh signifikan terhadap Belanja Daerah (BD) pada Provinsi Sumatera. Pendapatan Asli Daerah (PAD berpengaruh signifikan terhadap Pertumbuhan Ekonomi (PDRB) pada Provinsi Sumatera Barat. Dana Alokasi Umum (DAU), berpengaruh signifikan terhadap Pertumbuhan Ekonomi (PDRB) pada Provinsi Sumatera Barat. Belanja Daerah (BD) berpengaruh signifikan terhadap Pertumbuhan Ekonomi (PDRB) pada Provinsi Sumatera Barat. Pendapatan Asli Daerah (PAD), berpengaruh signifikan terhadap Pertumbuhan Ekonomi (PDRB) melalui Belanja Daerah (BD) sebagai Variabel Intervening pada Provinsi Sumatera. Dana Alokasi Umum (DAU), tidak signifikan terhadap Pertumbuhan Ekonomi (PDRB) melalui Belanja Daerah (BD) sebagai Variabel Intervening pada Provinsi Sumatera Barat Tahun Anggaran 2017-2021.
HUBUNGAN PERILAKU WARGA DI RUMAH DENGAN KONDISI LINGKUNGAN TEMPAT TINGGAL SEBAGAI FAKTOR PENYEBAB DEMAM BERDARAH DENGUE DI KELURAHAN KOTA UNENG KECAMATAN ALOK KABUPATEN SIKKA
Authors: Da Cunha Maria Febrina Carola , Samin Mikael , Rahmawati Arfita 
Published: October 2022

This study aims to determine the relationship between the behavior of residents at home and living environmental conditions as a factor causing dengue hemorrhagic fever seen from the behavior of residents at home that affects dengue hemorrhagic fever with environmental conditions where they live as a breeding ground for the aedes aegypti mosquito. The type of research used is descriptive quantitative research. Data collection techniques using questionnaires and observation. Respondents and places of observation in this study were 100 people and 100 houses in the Urban Village of Uneng. The positive analysis technique used in the study was univariate analysis to describe the behavioral variables of residents at home and mosquito population density with the formula for the positive proportion of houses with Aedes aegypty (HI) larvae, percentage of breeding sites with Aedes aegypty (CI) larvae, and number of breeding sites with Aedes aegypty larvae. positive for Aedes aegypti (BI) larvae and the number of houses that are not infected with larvae (ABJ). The results of the study indicate that there is a relationship between the behavior of residents at home and the condition of the living environment as a factor causing dengue hemorrhagic fever. This relationship is due to the dominant behavior of residents at home is the behavior of residents who influence the occurrence of dengue hemorrhagic fever against environmental conditions where the aedes aegypti mosquito larvae are positive. From this relationship, it was concluded that in Uneng City Village, it was very likely that residents at home could experience dengue hemorrhagic fever. This is based on the magnitude of the Bretau Index (BI) figure of 53% and the larva-free rate (ABJ) of 62%
Analysis of the effectiveness of the SIRS, qSOFA and MEWS scale for the sepsis identiﬁcation in oncohematological patients. Gematologiya i transfuziologiya
Authors: Lyanguzov A. V. , Luchinin A. S. 
Published: October 2022

Introduction. Sepsis is one of the main causes of high mortality in oncohematological patients. Sepsis is diagnosed using different scoring scales, but the accuracy of the diagnosis varies.Aim — to determine the effectiveness of SIRS, qSOFA and MEWS scales for the diagnosis of sepsis in oncohematological patients.Materials and methods. Study participants included 202 patients hospitalized in the intensive care unit with infectious complications — 112 (55 %) male, 90 (45 %) female. The median age was 57 years. The estimated predictors were the criteria of SIRS, qSOFA and MEWS scales and other significant indicators. The Boruta method was employed to select the predictors. A multiple binomial logistic regression model was used to determine the prognostic value of the predictors. The probability of sepsis was calculated based on the regression equation. The correlation between sepsis probability and the prognostic scales was assessed using correlation analysis (Kendall rank correlation coefficient).Results. Diagnoses were confirmed by scales in 95 of 202 (47 %) patients: SIRS — in 77 (81 %), qSOFA — in 31 (33 %), MEWS — in 65 (68 %). Of the 19 predictors initially included in the study, the final logistic regression model included 6: respiratory rate, heart rate, impaired urine output, systolic blood pressure, body temperature, and Glasgow coma scale level, which matched MEWS criteria. The median probability of sepsis was 0.38 (0.079–0.921). The results of the calculated probability of sepsis, according to the logistic regression model, correlated most closely with the score on the MEWS scale, to a lesser extent — with that on SIRS and qSOFA.Conclusion. The MEWS scale is a more suitable tool for the diagnosis of sepsis than SIRS and sofa in oncohematological patients.
Endothelial-platelet interaction in sepsis
Authors: Divakova Yu. V. , Koloskov A. V. 
Published: October 2022

Introduction. Endothelial cells and platelets are actively involved in pathogenetic processes in sepsis — an unregulated reaction of the host organism in response to infection, leading, on the one hand, to the development of prothrombotic, and on the other hand, to hemorrhagic readiness.Aim — to summarize the current information on the mechanisms of intercellular interaction between endotheliocytes and platelets in sepsis.Main findings. The development of multiple organ failure, which increases the likelihood of an unfavorable outcome of sepsis, is partly due to endothelial dysfunction, as well as the involvement of platelets in the pathogenetic process. Under physiological conditions, an anatomically and functionally intact endothelium is important to prevent microvascular thrombosis. Although platelets are most associated with hemostasis, they perform many other functions, including participation in inflammatory processes through complement activation, interaction with leukocytes and monocytes, participation in host defense against infection, and regulation of vascular tone. There is also abundant evidence that suggests that the processes regulating hemostasis evolved as a component of the inflammatory response to infection. Many of these interaction points occur on the surface of endothelial cells, linking these two cell types, endotheliocytes and platelets, in initiating and regulating blood clotting and inflammation. Various mechanisms may contribute to direct and indirect platelet activation in sepsis, including pathogen-induced platelet activation, pathogen- and inflammation-induced endothelial and leukocyte activation, and complement-mediated platelet activation.
Clinical guidelines for the management of patients with paroxysmal nocturnal hemoglobinuria
Authors: Savchenko V. G. , Lukina E. A. , Mikhaylova E. A. , Tsvetaeva N. V. , Latyshev V. D. , Lukina K. A. , Fidarova Z. T. , Galtseva I. V. , Dvirnik V. N. , Ptushkin V. V. , Afanasyev B. V. , Kulagin A. D. , Shilova E. R. , Maschan A. A. , Smetanina N. S. , Lugovskaya S. A. 
Published: October 2022

Introduction. Paroxysmal nocturnal hemoglobinuria (PNH) is a rare acquired clonal disease of the blood system characterized by intravascular hemolysis, bone marrow dysfunction and an increased risk of thrombotic and organ complications.Aim — to provide relevant clinical recommendations for the provision of medical care to adults and children with PNH.Basic information. Experts from the National Hematological Society association which is focused on the promotion of hematology, transfusiology and bone marrow transplantation along with experts from the public organization, National Society of Pediatric Hematologists and Oncologists, have developed current clinical recommendations for providing medical care to adults and children with PNH. The recommendations address in detail the issues of etiology, pathogenesis, epidemiology, and clinical manifestations of the disease. Special attention is paid to the diagnosis, differential diagnosis, and treatment of PNH based on the principles of evidence.
Practical Considerations for Engaging Youth With Lived And/or Living Experience of Substance Use as Youth Advisors and Co-Researchers
Authors: Turuba Roxanne , Irving Sarah , Turnbull Haley , Amarasekera Anurada , Howard Amanda Madeleine , Brockmann Violet , Tallon Corinne , Mathias Steve , Henderson Joanna , Barbic Skye 
Published: October 2022

Background Although youth participatory action research (YPAR) has been gaining popularity in the context of substance use research over the last decade, few studies report on how to apply these methods effectively with youth. To address this gap, this paper describes how we engaged youth as advisors and research assistants in a qualitative research study, as well as our key learnings and practical considerations for engaging youth with lived and/or living experience of substance use. Methods We applied YPAR methods by partnering with youth from across BC who have lived and/or living experience of substance use to form a project youth advisory that co-designed the research protocol and materials. Three members became youth research assistants to facilitate focus groups and qualitative interviews with other youth with lived/living experience and peer support workers who work with youth, and support with data analysis and the dissemination of research findings. Mid- and end-point surveys were distributed to evaluate and improve our youth engagement methods over the course of the study. Results The positive impact of engaging youth with lived/living experience over the course of the study was reflected by study participant responses to the quality and relevance of the focus group/interview questions and positive experiences with the youth facilitators. Youth partners also described developing new skills in research, group facilitation, leadership, communication, and professionalism. Lessons learned and considerations for engaging youth in substance use research included: an equitable hiring process; proper compensation; communication, transparency, and accountability; safe spaces; flexibility; and youth capacity building. Conclusion This study demonstrates varying ways of engaging youth with lived and/or living experience of substance in research and how youth contributions can improve the relevance, quality, and validity of the research. Lessons learned from this study can be applied by other researchers considering engaging youth with lived/living experience or other marginalized populations.
Design of a Random Number Generator for Stochastic Computing(SC)
Authors: Junsangsri Pilin , Lombardi Fabrizio 
Published: October 2022

<p> In most stochastic computing designs, the Stochastic Number Generator (SNG) circuit is of primary importance, but it may require considerable area due to its complexity. This paper proposes a new design for random number generators (RNGs) to be used in SNGs. The proposed RNG design exploits the randomness between each bit of data to generate larger sets of random numbers by concatenating the modules of the proposed customized linear feedback shift registers (LFSRs). To efficiently generate random data, a plane of RNGs (consisting of multiple modules) is proposed; a so-called sliding window approach is utilized for reading data in both directions; (horizontal and vertical). Therefore, sets of random numbers are generated by doubling these datasets and inverting the duplicated datasets. Flip-Flops (FFs) are then used to isolate the datasets and reduce the correlation among them. Compared to existing designs found in the technical literature, the proposed RNG design offers many advantages such as small area per RNG, low power operation, large number of datasets, flexibility and higher accuracy. When compared to the LFSR plane, the proposed RNG design improve power dissipation by 47.03%, area per RNG by 48.71% and the number of datasets by 1466%, the size of each random by 183,000% and the autocorrelation by 98.19%. The proposed design has been used in a stochastic multiplier and adder; for these circuits, the mean square error is reduced by 40.62% for the multiplier circuit, and 26.79% for the adder circuit. </p>
Impact of Channel Models on Performance Characterization of RIS-Assisted Wireless Systems
Authors: Jamali Vahid , Ghanem Walid. R. , Schober Robert , Poor H. Vincent 
Published: October 2022

<p>The performance characterization of communication systems assisted by large reconfigurable intelligent surfaces (RISs) significantly depends on the adopted models for the underlying channels. Under unrealistic channel models, the system performance may be over- or under-estimated which yields inaccurate conclusions for the system design. In this paper, we review five channel models that are chosen to progressively improve the modeling accuracy for large RISs. For each channel model, we highlight the underlying assumptions, its advantages, and its limitations. We compare the system performance under the aforementioned channel models using RIS configuration algorithms from the literature and a new scalable algorithm proposed in this paper specifically for the configuration of extremely large RISs.</p>
Machine Learning Based Thermal Evaluation for Vertically-Composed Fine-Grained 3D CMOS
Authors: Li Mingyu , Kulkarni Sourabh , Bhat Sachin , Moritz Csaba Andras 
Published: October 2022

<p>Thermal management in 3D integrated circuits is a critical challenge due to their high computational density. Heat dissipation paths from top circuit layers through bottom layers to substrate are heavily constraining heat extraction. Various thermal management frameworks have been proposed to address thermal issues in different granularities. All these frameworks require a thermal evaluation stage that characterizes the thermal profile of large designs with fast runtime. In this work, we present a machine learning based thermal evaluation method that predicts all standard cell temperatures based on features extracted from circuit CAD files. We have built thermal resistance networks for 10 benchmark circuits. We performed simulations to achieve the thermal data, and trained the thermal model with the data. The model is highly accurate and can identify all over-heated cells that need to be thermally-optimized. Runtime overhead is minimal. For a 435k-cell SPARC T2 core, the runtime for predicting all cell temperatures is as small as 3.12s, which is negligible compared to the runtime of other physical design stages. </p>
Blockchain MEV minimisation solution with price guarantee reward
Authors: Pillai Babu 
Published: October 2022

<p>Blockchain systems rely on miners to produce blocks, who solely build blocks for their financial benefit. Within the competitive market system, only correct blocks get accepted, which ensures security of the network. As financial applications migrate into the blockchain, miners in this privileged position have identified ways to extract additional revenues (known as MEV) that affect users and the overall security of the system. A possible direction for current research to resolve this exploitation is to separate the sequencing (building) and execution (mining/proposing) of transactions (PBS model). However, a weaker point of this model is the trust assumption between participants and the incentive model. This paper proposes a concept of separate incentive schemes for the work various participants conduct in their own interests.</p>