bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carboxymethyl cellulose/shellac composite loaded with pomegranate extract and jojoba oil as anti-mycotic and anti-mycotoxigenic food packaging materials
Authors: Mohamed Salah A. A. , Farouk Amr , Abdel-Razek Adel G. , Nashy El-Shahat , El-Sakhawy Mohamed , Badr Ahmed Noah 
Published: January 2025

AbstractFood commodities, including mycotoxins naturally produced from toxigenic fungi (pre- or post-harvest), are particularly vulnerable to contamination. The study intended to use unique bioactive composites loaded with antimicrobial constituents for food packaging. Three composite types are based on carboxymethyl cellulose/shellac (CMC/SH) and loaded with pomegranate extract (POE) with or without jojoba oil (JOE) at various concentrations. An enhancement was recorded for tensile strength and elongation at break and burst properties of the composites, where the results point out the amelioration of flexibility and elasticity with E9 (0.3/3 mg/mL of POE/JO). Moreover, E10 (0.3/1 of POE/JOE) content had higher phenolic and flavonoids, with significant antioxidants and the best antimicrobial and anti-mycotoxigenic activity. Six higher antimicrobial composites were chosen for corn seed coating applications in a simulated experiment of toxigenic fungal contamination, where the results recommend E10 as the best formula for packaging application. The E10 was characterized for emulsion stability, particle size, zeta potential, pH, PDI, and acidity that were recorded at 88.16 ± 2.87%, 54.81 nm, 38.74 mV, 6.34 ± 0.54, 31.12 ± 1.02, and 6.02 ± 0.34 mg/L, respectively. The in-silico study revealed that ellagic acid and hesperidin in POE extract, erucic and oleic acids in JOE, and shellac had the highest binding free energies against the vital enzymes involved in bactericidal/bacteriostatic effects and the aflatoxin bio synthetic mechanism.
Unveiling the potential anticancer activity of Spirulina maxima extract-nanoemulsion through in vitro and in vivo studies
Authors: Hussein Mohammed Yasser , Nasr Merna , Emad Veronia , Maged Julie , George Portia , Emad Amina , Badr Abeer Mahmoud , El-Naggar Mehrez E. , Abdo Sayeda M. , Hussein Jihan 
Published: January 2025

AbstractBeing the second leading cause of death globally, cancer has been a long-standing and rapidly evolving focus of biomedical research and practice in the world. Recently, there has been growing interest in cyanobacteria. This focus is particularly evident in developing innovative anticancer treatments to reduce reliance on traditional chemotherapy. This study investigates the anticancer potential of the Spirulina maxima extract nanoemulsion (SMNE) technique to improve the delivery, stability, and solubility of the S. maxima extract (SME). SMNE, prepared in three concentrations (SMNEC1, SMNEC2, SMNEC3), was characterized and confirmed to successfully load SME into silica-coated nanoparticles. Cytotoxicity tests on HepG2 and MCF-7 cell lines revealed a significant reduction in cell viability after 48-hour SMNE treatment, with IC50 values of 1488 µg/mL and 1721.936 µg/mL, respectively. SMNE also demonstrated efficacy in inhibiting tumor growth in mice with Ehrlich ascites carcinoma, normalizing alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, and reducing oxidative stress markers such as catalase (CAT) and malondialdehyde (MDA). Histopathological examination showed that SMNEC3-treated groups had almost normal liver architecture. Additionally, SMNE downregulated oncogenic miR-221-3p and miR-222-3p, activating cancer suppression genes p27 and PTEN. The study concludes that SMNE, with its anti-inflammatory and antioxidant properties and ability to modulate key miRNAs, enhances SME delivery and shows promise as an effective cancer treatment.
Two-stage multi-objective framework for optimal operation of modern distribution network considering demand response program
Authors: Elshenawy Mohamed R. , Mohamed Abdalla , Ali A. A. , Mosa Magdi A. 
Published: January 2025

AbstractTo improve the inadequate reliability of the grid that has led to a worsening energy crisis and environmental issues, comprehensive research on new clean renewable energy and efficient, cost-effective, and eco-friendly energy management technologies is essential. This requires the creation of advanced energy management systems to enhance system reliability and optimize efficiency. Demand-side energy management systems are a superior solution for multiple reasons. Firstly, they empower consumers to actively oversee and regulate their energy consumption, resulting in substantial cost savings by minimizing usage during peak hours and enhancing overall efficiency. The Demand Response Program (DRP) and optimal power sharing have gained significant attention to provide technical and economic benefits, while they require an efficient operation framework. Therefore, a two-stage framework is proposed for multi-objective operation of a distribution network with several generation resources. The first stage applies DRP to maximize the distribution network operator’s (DNO) profit by optimizing common incentive rate for all consumers participate in DRP and an individual curtailed power for each consumer. In addition to an individual incentive rate for each consumer participates in DRP which is a new solution in the field of demand side management. The second stage achieves optimal power sharing among generation resources, while considering multiple objectives and incorporating the modified load of the first stage. The multi-objective problem is formulated to reduce energy losses, voltage deviation, total operational cost, gas emissions, and maximize the voltage stability index. The problem is optimized using a combination of the technique for order of preference by similarity to ideal solution (TOPSIS) and the elephant herding optimization (EHO) technique. The framework is validated using a modified IEEE 33-bus that incorporates photovoltaic system, diesel generators, and wind generation system. The proposed framework based on an individual incentive rate DRP provides superior response compared to common incentive rate DRP which reduces the total energy losses by 38.13%, reduces the total generation cost by 9.468%, and reduces the emission by 5.9%.
Polycystic ovary syndrome negatively affects sexual function and lower urinary tract symptoms in syrian women: a case-control study
Authors: Alshiekh Ali , Hadakie Rana , Al Kurdi M Fadi , sukkar Luna , Alhalabi Marwan , Hamed Hamoud 
Published: January 2025

AbstractPolycystic ovary syndrome (PCOS) is the most prevalent endocrine disorder in women of reproductive age worldwide, and its related features like obesity, mental health issues and hyperandrogenism may contribute to inadequately investigated health problems such as sexual dysfunction (SD) and lower urinary tract symptoms (LUTS). Therefore, this study examined the impact of PCOS on sexual function (SF) and lower urinary tract in Syrian women by recruiting a total of 178 women of reproductive age, of whom 88 were diagnosed with PCOS according to the Rotterdam criteria and 90 without PCOS were considered as the control group. Female sexual function index (FSFI) and Bristol Female Lower Urinary Tract Symptom Questionnaire (BFLUTS) were used to assess SF and LUTS respectively. PCOS group had higher SD prevalence compared to control group (65.9% vs 48.9%, p = 0.016), and BMI showed an inverse correlation with the total FSFI score in PCOS group (p = 0.027, r = -0.235). Furthermore, PCOS group exhibited significantly lower scores in orgasm and satisfaction subdomains. Additionally, PCOS patients had significantly higher total BFLUTS score compared to control group (median 8 vs 5, p = 0.025). Thus, PCOS may be related to SD and LUTS, highlighting the importance of evaluating SF and urinary symptoms in PCOS patients.
Data-driven strategies to improve nitrogen use efficiency of rice farming in South Asia
Authors: Coggins Sam , McDonald Andrew J. , Silva João Vasco , Urfels Anton , Nayak Hari Sankar , Sherpa Sonam Rinchen , Jat Mangi Lal , Jat Hanuman Sahay , Krupnik Tim , Kumar Virender , Malik Ram. K. , Sapkota Tek B. , Nayak Amaresh Kumar , Craufurd Peter 
Published: January 2025

Abstract
Increasing nitrogen use efficiency (NUE) in agricultural production mitigates climate change, limits water pollution and reduces fertilizer subsidy costs. Nevertheless, strategies for increasing NUE without jeopardizing food security are uncertain in globally important cropping systems. Here we analyse a novel dataset of more than 31,000 farmer fields spanning the Terai of Nepal, Bangladesh’s floodplains and four major rice-producing regions of India. Results indicate that 55% of rice farmers overuse nitrogen fertilizer, and hence the region could save 18 kg of nitrogen per hectare without compromising rice yield. Disincentivizing this excess nitrogen application presents the most impactful pathway for increasing NUE. Addressing yield constraints unrelated to crop nutrition can also improve NUE, most promisingly through earlier transplanting and improving water management, and this secondary pathway was overlooked in the IPCC’s 2022 report on climate change mitigation. Combining nitrogen input reduction with changes to agronomic management could increase rice production in South Asia by 8% while reducing environmental pollution from nitrogen fertilizer, measured as nitrogen surplus, by 36%. Even so, opportunities to improve NUE vary within South Asia, which necessitates sub-regional strategies for sustainable nitrogen management.
RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and aggravates liver tumorigenesis
Authors: Zhang Fengwu , Xiong Xiufang , Li Zhijian , Wang Haibo , Wang Weilin , Zhao Yongchao , Sun Yi 
Published: January 2025

Abstract
Small GTPase RHEB is a well-known mTORC1 activator, whereas neddylation modifies cullins and non-cullin substrates to regulate their activity, subcellular localization and stability. Whether and how RHEB is subjected to neddylation modification remains unknown. Here, we report that RHEB is a substrate of NEDD8-conjugating E2 enzyme UBE2F. In cell culture, UBE2F depletion inactivates mTORC1, inhibiting cell cycle progression, cell growth and inducing autophagy. Mechanistically, UBE2F cooperates with E3 ligase SAG in neddylation of RHEB at K169 to enhance its lysosome localization and GTP-binding affinity. Furthermore, liver-specific Ube2f knockout attenuates steatosis and tumorigenesis induced by Pten loss in an mTORC1-dependent manner, suggesting a causal role of UBE2F in liver tumorigenesis. Finally, UBE2F expression levels and mTORC1 activity correlate with patient survival in hepatocellular carcinoma. Collectively, our study identifies RHEB as neddylation substrate of the UBE2F-SAG axis, and highlights the UBE2F-SAG axis as a potential target for the treatment of non-alcoholic fatty liver disease and hepatocellular carcinoma.
Two FAM134B isoforms differentially regulate ER dynamics during myogenesis
Authors: Buonomo Viviana , Lohachova Kateryna , Reggio Alessio , Cano-Franco Sara , Cillo Michele , Santorelli Lucia , Venditti Rossella , Polishchuk Elena , Peluso Ivana , Brunello Lorene , Cirillo Carmine , Petrosino Sara , Silva Malan , De Cegli Rossella , Di Bartolomeo Sabrina , Gargioli Cesare , Swuec Paolo , Cortese Mirko , Stolz Alexandra , Bhaskara Ramachandra M , Grumati Paolo 
Published: January 2025

Abstract
Endoplasmic reticulum (ER) plasticity and ER-phagy are intertwined processes essential for maintaining ER dynamics. We investigated the interplay between two isoforms of the ER-phagy receptor FAM134B in regulating ER remodeling in differentiating myoblasts. During myogenesis, the canonical FAM134B1 is degraded, while its isoform FAM134B2 is transcriptionally upregulated. The switch, favoring FAM134B2, is an important regulator of ER morphology during myogenesis. FAM134B2 partial reticulon homology domain, with its rigid conformational characteristics, enables efficient ER reshaping. FAM134B2 action increases in the active phase of differentiation leading to ER restructuring via ER-phagy, which then reverts to physiological levels when myotubes are mature and the ER is reorganized. Knocking out both FAM134B isoforms in myotubes results in an aberrant proteome landscape and the formation of dilated ER structures, both of which are rescued by FAM134B2 re-expression. Our results underscore how the fine-tuning of FAM134B isoforms and ER-phagy orchestrate the ER dynamics during myogenesis providing insights into the molecular mechanisms governing ER homeostasis in muscle cells.
A multiplex method for rapidly identifying viral protease inhibitors
Authors: Hong Seo Jung , Resnick Samuel J , Iketani Sho , Cha Ji Won , Albert Benjamin Alexander , Fazekas Christopher T , Chang Ching-Wen , Liu Hengrui , Dagan Shlomi , Abagyan Michael R , Fajtová Pavla , Culbertson Bruce , Brace Brooklyn , Reddem Eswar R , Forouhar Farhad , Glickman J Fraser , Balkovec James M , Stockwell Brent R , Shapiro Lawrence , O’Donoghue Anthony J , Sabo Yosef , Freundlich Joel S , Ho David D , Chavez Alejandro 
Published: January 2025

Abstract
With current treatments addressing only a fraction of pathogens and new viral threats constantly evolving, there is a critical need to expand our existing therapeutic arsenal. To speed the rate of discovery and better prepare against future threats, we establish a high-throughput platform capable of screening compounds against 40 diverse viral proteases simultaneously. This multiplex approach is enabled by using cellular biosensors of viral protease activity combined with DNA-barcoding technology, as well as several design innovations that increase assay sensitivity and correct for plate-to-plate variation. Among >100,000 compound-target interactions explored within our initial screen, a series of broad-acting inhibitors against coronavirus proteases were uncovered and validated through orthogonal assays. A medicinal chemistry campaign was performed to improve one of the inhibitor’s potency while maintaining its broad activity. This work highlights the power of multiplex screening to efficiently explore chemical space at a fraction of the time and costs of previous approaches.
Macrophage-induced enteric neurodegeneration leads to motility impairment during gut inflammation
Authors: Breßer Mona , Siemens Kevin D , Schneider Linda , Lunnebach Jonah E , Leven Patrick , Glowka Tim R , Oberländer Kristin , De Domenico Elena , Schultze Joachim L , Schmidt Joachim , Kalff Jörg C , Schneider Anja , Wehner Sven , Schneider Reiner 
Published: January 2025

Abstract
Current studies pictured the enteric nervous system and macrophages as modulators of neuroimmune processes in the inflamed gut. Expanding this view, we investigated the impact of enteric neuron–macrophage interactions on postoperative trauma and subsequent motility disturbances, i.e., postoperative ileus. In the early postsurgical phase, we detected strong neuronal activation, followed by transcriptional and translational signatures indicating neuronal death and synaptic damage. Simultaneously, our study revealed neurodegenerative profiles in macrophage-specific transcriptomes after postoperative trauma. Validating the role of resident and monocyte-derived macrophages, we depleted macrophages by CSF-1R-antibodies and used CCR2−/− mice, known for reduced monocyte infiltration, in POI studies. Only CSF-1R-antibody-treated animals showed decreased neuronal death and lessened synaptic decay, emphasizing the significance of resident macrophages. In human gut samples taken early and late during abdominal surgery, we substantiated the mouse model data and found reactive and apoptotic neurons and dysregulation in synaptic genes, indicating a species’ overarching mechanism. Our study demonstrates that surgical trauma activates enteric neurons and induces neurodegeneration, mediated by resident macrophages, introducing neuroprotection as an option for faster recovery after surgery.
Structural-morphological insights into optimization of hydrothermally synthesized MoSe2 nanoflowers for improving supercapacitor applications
Authors: Yadav Poonam , Yadav Rohit , Pani Jitesh , Singh Ram Mehar , Singh Davender , Kumari Kusum , Borkar Hitesh , Gangwar Jitendra 
Published: January 2025

Present work reports an improved hydrothermal methodology for synthesis of MoSe2 nanoflowers (MNFs) at 210 °C (±10 °C). Maximum specific capacitance of 284.8 F g−1 at 5 mV s−1 scan rate indicating the MNFs as an electrode material for supercapacitors.
Anionic modulation induces molecular polarity in a three-component crown ether system
Authors: Liu Feiyan , Chen Guoyong , Liu Xiao , Jiang Ting , Guo Wenjing , Li Xiancai , Wei Zhenhong 
Published: January 2025

We synthesized two novel host-guest crown ether molecules [(PTFMA)(15-crown-5)ClO4] (1) and [(PTFMA)(15-crown-5)PF6] (2). Compound 1 transitioned from a non-centrosymmetric space group to a centrosymmetric one.
What Matters When Providing Conversational Recast Treatment? A Multilevel Modeling Analysis
Authors: Choi-Tucci Alexander , Sachs Alyssa , Burton Rebecca , Vance Rebecca , Plante Elena 
Published: January 2025

Purpose:
Conversational recasting treatment is generally effective. However, different versions of this treatment and different targets may yield different outcomes for children. Here, we directly compare multiple variations of conversational recasting to determine how modifications to delivery and target impact treatment outcomes.

Method:
Using Bayesian hierarchical linear mixed modeling, we compared outcome data from 141 children with developmental language disorder (DLD) across 10 versions of recast treatment compared with enhanced conversational recast treatment (a version that incorporates linguistic variability and attentional cues). We also compared data from a subset of 132 children treated for one of five morphological targets to determine the relative difficulty of learning each target.

Results:
Results suggest that seven of the 10 treatment conditions resulted in an average decrease in generalization probe performance relative to enhanced conversational recasting alone. These conditions resulted in probe performance that was between 47% and 84% worse than performance of children who received enhanced conversational recasting with no variations. The remaining three conditions were no better or worse than enhanced conversational recasting. One treatment target was easier to remediate, while the other four were no easier or harder to acquire through treatment.

Conclusions:
These results suggest that different variations of conversational recasting facilitate or diminish learning by children with DLD and that clinicians' attention to the form and structure of treatment delivery is necessary to implement best practice for this treatment method. In contrast, clinicians may have more flexibility when selecting targets.

Supplemental Material:

https://doi.org/10.23641/asha.28055207
Short-Term Effects of Semi-Occluded Vocal Tract Therapy on the Phonation of Children With Vocal Fold Nodules: A Randomized Controlled Trial
Authors: Adriaansen Anke , Meerschman Iris , Van Lierde Kristiane , Claeys Sofie , Ma Estella P.-M. , Kissel Imke , Papeleu Tine , D'haeseleer Evelien 
Published: January 2025

Purpose:
The aim was to determine and compare the short-term effects of two intensive semi-occluded vocal tract (SOVT) programs, “straw phonation” (SP) and “resonant voice therapy” (RVT), on the phonation of children with vocal fold nodules.

Method:

A pretest–posttest randomized controlled study design was used. Thirty children aged 6–12 years were randomly assigned to the SP group (
n
= 11), RVT group (
n
= 11), or control group receiving indirect treatment (
n
= 8) for their voice problems. All participants received 11 hr of group voice therapy over four consecutive days. A multidimensional voice assessment consisting of both objective (dysphonia severity index and acoustic voice quality index) and subjective (pediatric voice handicap index and perceptual rating of overall severity) measures was performed pre- and posttherapy. Voice therapy effectiveness was evaluated using group-level analyses (linear mixed models) and individual-level analyses to investigate what proportion of participants changed to a clinically relevant degree.

Results:
Group-level analyses found no significant Time × Group interactions, indicating that the evolution over time did not differ among the three groups. Within-group effects of time showed a significant and equal improvement in dysphonia severity index in the SP and RVT groups and a significant improvement in perceptual rating of overall severity in the SP group. For dysphonia severity index, individual-level analyses showed that 36% and 45% of participants improved to a clinically relevant degree in the SP and RVT groups, respectively. For acoustic voice quality index, 38% improved to a clinically relevant degree in the SP group.

Conclusions:
Results suggest that short-term intensive SOVT programs may have a positive effect on voice quality and vocal capacities of children with vocal fold nodules. Participants seem to benefit more from a SP program than a RVT program.
Random Strip Peeling: A novel lightweight image encryption for IoT devices based on colour planes permutation
Authors: İnce Kenan , İnce Cemile , Hanbay Davut 
Published: January 2025

AbstractThis paper introduces a novel lightweight colour image encryption algorithm, specifically designed for resource‐constrained environments such as Internet of Things (IoT) devices. As IoT systems become increasingly prevalent, secure and efficient data transmission becomes crucial. The proposed algorithm addresses this need by offering a robust yet resource‐efficient solution for image encryption. Traditional image encryption relies on confusion and diffusion steps. These stages are generally implemented linearly, but this work introduces a new RSP (Random Strip Peeling) algorithm for the confusion step, which disrupts linearity in the lightweight category by using two different sequences generated by the 1D Tent Map with varying initial conditions. The diffusion stage then employs an XOR matrix generated by the Logistic Map. Different evaluation metrics, such as entropy analysis, key sensitivity, statistical and differential attacks resistance, and robustness analysis demonstrate the proposed algorithm's lightweight, robust, and efficient. The proposed encryption scheme achieved average metric values of 99.6056 for NPCR, 33.4397 for UACI, and 7.9914 for information entropy in the SIPI image dataset. It also exhibits a time complexity of for an image of size .
Travelling wave‐based fault detection and location in a real low‐voltage DC microgrid
Authors: Paruthiyil Sajay Krishnan , Bidram Ali , Aparicio Miguel Jimenez , Hernandez‐Alvidrez Javier , Dow Andrew R. R. , Reno Matthew J. , Bauer Daniel 
Published: January 2025

AbstractThis paper discusses a device‐level implementation of a travelling wave (TW) protection device (PD) designed for a real low‐voltage DC microgrid. The TWPD fault detection and location algorithm is executed on a commercial digital signal processor (DSP) board, involving signal sampling at 1 MHz via the DSP board's analog‐to‐digital converter (ADC). The analogue input card measures positive pole, negative pole and pole‐to‐pole voltages at the TWPD location. Upon a successful fault detection using a second‐order high‐pass filter, the voltage data is normalised and multi‐resolution analysis (MRA) is performed on a 128‐sample buffer around the TW arrival time. MRA employs the discrete wavelet transform (DWT) to capture high‐frequency voltage patterns, and then the Parseval's energy theorem quantifies these TW characteristics by computing the energy of reconstructed wavelet coefficients. These energy values per decomposed frequency band are the basis for training a random forest classifier that predicts fault location and type. The TWPD is fully implemented and connected to a real DC microgrid in Albuquerque, NM, USA, for validation, and results are shown for field tests verifying the performance under faults.