bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Characteristics, Co-Mutations, and Treatment Outcomes in Advanced Non-Small-Cell Lung Cancer Patients With the BRAF-V600E Mutation
Authors: Qu Jingjing , Shen Qian , Li Yuping , Kalyani Farhin Shaheed , Liu Li , Zhou Jianya , Zhou Jianying 
Published: June 2022

BackgroundLimited treatment outcome data is available for advanced non-small cell lung cancer (NSCLC) patients with BRAF V600E mutations. In this multicenter study, we describe therapeutic options and survival outcomes for patients with mutated BRAF V600E.MethodThis was a retrospective study in which BRAF V600E-mutated advanced NSCLC patients were retrospectively recruited between January 2015 and December 2021 and had their clinical characteristics, co-mutations, and treatment efficacy assessed.ResultsFifty-three patients with BRAF V600E-mutant advanced NSCLC were included in the study, of which 64.2% were non-smokers, and the BRAF V600E mutation was more prevalent in men (52.8%). In addition, 96.2% of the patients had adenocarcinoma, and most (96.2%) received first-line therapy (23.5% anti-BRAF), with a progression-free survival (PFS) and overall survival (OS) of 10.0 [95% confidence interval (CI): 1.5–36.0 months] and 24.0 months [95% CI: 3.0–53.0 months], respectively. Twenty-three patients (43.4%) received second-line treatment (39.1% anti-BRAF), and PFS and OS were 5.0 [95% CI: 1.0–21.0 months] and 13.0 months [95% CI: 1.5–26.0 months], respectively. BRAF and MEK-targeted therapy (dabrafenib plus trametinib) produced longer PFS compared with that of chemotherapy with or without bevacizumab as a first-line (NA vs. 4.0 months, P = 0.025) or second-line therapy (6.0 vs. 4.6 months, P = 0.017). NSCLC patients harboring driver oncogene mutations such as BRAF V600E, EGFR, or ALK should be treated using targeted therapies. Concurrent TP53 mutations were the most common, affecting 11.3% (n = 6) of the patients, followed by EGFR 19 Del (n = 5). Patients with concurrent mutations had shorter PFS (9.0 vs. 10.0 months, P = 0.875) and OS (14.0 vs. 15.0 months, P = 0.555) than those without these mutations.ConclusionThese results suggest that combined BRAF- and MEK-targeted therapy is effective in BRAF V600E-mutated advanced NSCLC patients. Dabrafenib and trametinib re-challenge is also an option for patients with BRAF V600E-mutated NSCLC.
Mechanism of Neural Regeneration Induced by Natural Product LY01 in the 5×FAD Mouse Model of Alzheimer’s Disease
Authors: Li Xiao-Wan , Lu Yang-Yang , Zhang Shu-Yao , Sai Ning-Ning , Fan Yu-Yan , Cheng Yong , Liu Qing-Shan 
Published: June 2022

Background: A sharp decline in neural regeneration in patients with Alzheimer’s disease (AD) exacerbates the decline of cognition and memory. It is of great significance to screen for innovative drugs that promote endogenous neural regeneration. Cytisine N-methylene-(5,7,4′-trihydroxy)-isoflavone (LY01) is a new compound isolated from the Chinese herbal medicine Sophora alopecuroides with both isoflavone and alkaloid characteristic structures. Its pharmacological effects are worth studying.Objective: This study was designed to determine whether LY01 delays the cognitive and memory decline in the early stage of AD and whether this effect of LY01 is related to promoting neural regeneration.Methods: Eight-week-old 5×Familial Alzheimer’s Disease (5×FAD) mice were used as disease models of early AD. Three doses of LY01 administered in two courses (2 and 5 weeks) of treatment were tested. Cognition, memory, and anxiety-like behaviors in mice were evaluated by the Morris water maze, fear conditioning, and open field experiments. Regeneration of neurons in the mouse hippocampus was observed using immunofluorescence staining. The effect of LY01 on cell regeneration was also demonstrated using a series of tests on primary cultured neurons, astrocytes, and neural stem cells (NSCs). In addition, flow cytometry and transcriptome sequencing were carried out to preliminarily explored the mechanisms.Results: We found that LY01 reduced the decline of cognition and memory in the early stage of 5×FAD mice. This effect was related to the proliferation of astrocytes, the proliferation and migration of NSCs, and increases in the number of new cells and neural precursor cells in the dentate gyrus area of 5×FAD mice. This phenomenon could be observed both in 2-week-old female and 5-week-old male LY01-treated 5×FAD mice. The neuronal regeneration induced by LY01 was related to the regulation of the extracellular matrix and associated receptors, and effects on the S phase of the cell cycle.Conclusion: LY01 increases the proliferation of NSCs and astrocytes and the number of neural precursor cells in the hippocampus, resulting in neural regeneration in 5×FAD mice by acting on the extracellular matrix and associated receptors and regulating the S phase of the cell cycle. This provides a new idea for the early intervention and treatment of AD.
A Novel Quantitative Arousal-Associated EEG-Metric to Predict Severity of Respiratory Distress in Obstructive Sleep Apnea Patients
Authors: S Malatantis-Ewert , K Bahr , H Ding , Ludwig Katharina , N Koirala , T Huppertz , H Gouveris , M Muthuraman 
Published: June 2022

Respiratory arousals (RA) on polysomnography (PSG) are an important predictor of obstructive sleep apnea (OSA) disease severity. Additionally, recent reports suggest that more global indices of desaturation such as the hypoxic burden, namely the area under the curve (AUC) of the oxygen saturation (SaO2) PSG trace may better depict the desaturation burden in OSA. Here we investigated possible associations between a new metric, namely the AUC of the respiratory arousal electroencephalographic (EEG) recording, and already established parameters as the apnea/hypopnea index (AHI), arousal index and hypoxic burden in patients with OSA. In this data-driven study, polysomnographic data from 102 patients with OSAS were assessed (32 female; 70 male; mean value of age: 52 years; mean value of Body-Mass-Index-BMI: 31 kg/m2). The marked arousals from the pooled EEG signal (C3 and C4) were smoothed and the AUC was estimated. We used a support vector regressor (SVR) analysis to predict AHI, arousal index and hypoxic burden as captured by the PSG. The SVR with the arousal-AUC metric could quite reliably predict the AHI with a high correlation coefficient (0,58 in the training set, 0,65 in the testing set and 0,64 overall), as well as the hypoxic burden (0,62 in the training set, 0,58 in the testing set and 0,59 overall) and the arousal index (0,58 in the training set, 0,67 in the testing set and 0,66 overall). This novel arousal-AUC metric may predict AHI, hypoxic burden and arousal index with a quite high correlation coefficient and therefore could be used as an additional quantitative surrogate marker in the description of obstructive sleep apnea disease severity.
Not all Forms of Muscle Hypertonia Worsen With Fatigue: A Pilot Study in Para Swimmers
Authors: Puce Luca , Bragazzi Nicola Luigi , Currà Antonio , Marinelli Lucio , Mori Laura , Cotellessa Filippo , Chamari Karim , Ponzano Marta , Samanipour Mohammad Hossein , Nikolaidis Pantelis T. , Biz Carlo , Ruggieri Pietro , Trompetto Carlo 
Published: June 2022

In hypertonic muscles of patients with upper motor neuron syndrome (UMNS), investigation with surface electromyography (EMG) with the muscle in a shortened position and during passive muscle stretch allows to identify two patterns underlying hypertonia: spasticity and spastic dystonia. We recently observed in Para swimmers that the effect of fatigue on hypertonia can be different from subject to subject. Our goal was, therefore, to understand whether this divergent behavior may depend on the specific EMG pattern underlying hypertonia. We investigated eight UMNS Para swimmers (five men, mean age 23.25 ± 3.28 years), affected by cerebral palsy, who presented muscle hypertonia of knee flexors and extensors. Muscle tone was rated using the Modified Ashworth Scale (MAS). EMG patterns were investigated in rectus femoris (RF) and biceps femoris (BF) before and after two fatiguing motor tasks of increasing intensity. Before the fatiguing tasks, two subjects (#2 and 7) had spasticity and one subject (#5) had spastic dystonia in both RF and BF. Two subjects (#3 and 4) showed spasticity in RF and spastic dystonia in BF, whereas one subject (#1) had spasticity in RF and no EMG activity in BF. The remaining two subjects (#6 and 8) had spastic dystonia in RF and no EMG activity in BF. In all the 16 examined muscles, these EMG patterns persisted after the fatiguing tasks. Spastic dystonia increased (p &lt; 0.05), while spasticity did not change (p &gt; 0.05). MAS scores increased only in the muscles affected by spastic dystonia. Among the phenomena possibly underlying hypertonia, only spastic dystonia is fatigue-dependent. Technical staff and medical classifiers should be aware of this specificity, because, in athletes with spastic dystonia, intense and prolonged motor activity could negatively affect competitive performance, creating a situation of unfairness among Para athletes belonging to the same sports class.
Cannabinoids in the Treatment of Cannabis Use Disorder: Systematic Review of Randomized Controlled Trials
Authors: Vuilleumier Caroline , Scherbaum Norbert , Bonnet Udo , Roser Patrik 
Published: June 2022

BackgroundThe prevalence of cannabis use and cannabis use disorders (CUD) has significantly increased over time. However, there are no approved pharmacological treatments for CUD. The aim of this study was to determine the efficacy and safety of various medical cannabinoids in the treatment of CUD.MethodsWe conducted a systematic review of randomized controlled trials which evaluated the therapeutic potential of medical cannabinoids in individuals with CUD and summarized the main study outcomes in terms of cannabis use, abstinence, withdrawal symptoms, craving, retention in treatment and adverse events.ResultsWe identified eight trials with a total of 667 study participants. Dronabinol reduced cannabis withdrawal symptoms whereas nabiximols, cannabidiol and PF-04457845, a fatty acid amide inhibitor, also reduced cannabis use and improved abstinence, compared to placebo. Nabilone failed to demonstrate efficacy in the treatment of CUD. All medications were well-tolerated.ConclusionsCannabinoid receptor agonists, i.e., dronabinol and nabilone, showed only limited or no therapeutic potential in the treatment of CUD. In contrast, modulators of endocannabinoid activity, i.e., nabiximols, cannabidiol and PF-04457845, demonstrated broader efficacy which covered almost all aspects of CUD. Endocannabinoid modulation appears to be a promising treatment approach in CUD, but the evidence to support this strategy is still small and future research in this direction is needed.
Prevalence and Unmet Need for Mental Healthcare of Major Depressive Disorder in Community-Dwelling Chinese People Living With Vision Disability
Authors: Zhong Bao-Liang , Xu Yan-Min , Li Yi 
Published: June 2022

ObjectiveMental health services have been recognized as an essential part of the comprehensive eye care services, but data regarding the mental health of people living with vision disability (PLwVD) remain very limited. This study examined the epidemiology of major depressive disorder (MDD) among Chinese PLwVD, as well as their perceived needs for and utilization of mental health services.MethodsBy using stratified cluster sampling method, a total of 1,753 PLwVD were successfully recruited from 73 urban communities and 169 rural villages in Wuhan, China, and interviewed with the Mini-international Neuropsychiatric Interview 5.0. Standardized questions were used to measure perceived mental healthcare needs and use of mental health services of PLwVD with MDD.ResultsThe one-month prevalence of MDD was 24.4%. Among the PLwVD with MDD, 26.0% perceived needs for mental healthcare and only 1.2% sought treatment from mental health specialists for their emotional problems. Factors associated with MDD were middle age [vs. young adulthood, odds ratio (OR) = 1.96, P &lt; 0.001], older adulthood (vs. young adulthood, OR = 1.79, P = 0.004), being never-married (vs. married, remarried, and cohabiting, OR = 1.96, P &lt; 0.001), being separated, divorced, and widowed (vs. married, remarried, and cohabiting, OR = 12.30, P &lt; 0.001), a low level of objective social support (vs. high, OR = 1.83, P &lt; 0.001), currently drinking (OR = 1.81, P &lt; 0.001), having childhood-onset eye conditions (OR = 1.89, P = 0.005), and having difficulties in performing daily activities (OR = 2.78, P &lt; 0.001).ConclusionsChinese PLwVD are at high risk for MDD and have a high level of unmet need for mental healthcare. Public strategies are warranted to improve the mental health literacy of PLwVD and make the mental health services available, accessible, and affordable for PLwVD.
Mediating Effects of Adolescent Physical Activity, Self-Rated Health and Family Income
Authors: Wang Meng , Shen Xi , Deng Lamei , Yu Feng , Lou Yin , Liu JunJi , Huang Yibing 
Published: June 2022

ObjectiveTo investigate and analyse the situation and relationship between adolescent physical activity and self-assessment of health to provide a reference for adolescent physical activity research.MethodsA questionnaire was used to investigate the physical activity and self-rated health of 1,804 adolescents aged 14–18 years.ResultsThere was a significant relationship between adolescents' physical activity and self-rated health. The coefficient was 0.109 (P &lt; 0.01) in urban areas and 0.127 (P &lt; 0.01) in rural areas. At the same time, it was found that when family income was used as the intermediary variable between physical activity and self-rated health, the intermediary effect was 0.12 (P &lt; 0.01), and the intermediary effect accounted for 25.97%.ConclusionAdolescent obesity, physical activity, smoking, wellbeing and physical activity can affect adolescents' self-rated health status. At the same time, it is also found that adolescents' family income is an intermediary variable between physical activity and self-rated health.Suggestions(1) Increase the methods of sports venues, sports organizations and sports activities, improve the possibility of teenagers participating in physical activities, and improve teenagers' self-rated health; (2) There is a large gap between the physical activity and self-rated health of urban and rural adolescents. Increasing the guidance of physical activity of adolescents in rural areas promotes the balance of self-rated health of urban and rural adolescents. (3) Family income is the intermediary variable of teenagers' physical activities and self-rated health. Reducing family expenditure through financial transfer payments or reducing taxes and fees can increase the level of teenagers' physical and mental health.
Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study
Authors: Merino-Ribas Ana , Araujo Ricardo , Pereira Luciano , Campos Joana , Barreiros Luísa , Segundo Marcela A. , Silva Nádia , Costa Carolina F. F. A. , Quelhas-Santos Janete , Trindade Fábio , Falcão-Pires Inês , Alencastre Ines , Dumitrescu Ioana Bancu , Sampaio-Maia Benedita 
Published: June 2022

Vascular calcification (VC) is a frequent condition in chronic kidney disease (CKD) and a well-established risk factor for the development of cardiovascular disease (CVD). Gut dysbiosis may contribute to CVD and inflammation in CKD patients. Nonetheless, the role of gut and blood microbiomes in CKD-associated VC remains unknown. Therefore, this pilot study aimed to explore the link between gut and blood microbiomes and VC in CKD patients on peritoneal dialysis (CKD-PD). Our results showed relative changes in specific taxa between CKD-PD patients with and without VC, namely Coprobacter, Coprococcus 3, Lactobacillus, and Eubacterium eligens group in the gut, and Cutibacterium, Pajaroellobacter, Devosia, Hyphomicrobium, and Pelomonas in the blood. An association between VC and all-cause mortality risk in CKD-PD patients was also observed, and patients with higher mortality risk corroborate the changes of Eubacterium eligens in the gut and Devosia genus in the blood. Although we did not find differences in uremic toxins, intestinal translocation markers, and inflammatory parameters among CKD-PD patients with and without VC, soluble CD14 (sCD14), a nonspecific marker of monocyte activation, positively correlated with VC severity. Therefore, gut Eubacterium eligens group, blood Devosia, and circulating sCD14 should be further explored as biomarkers for VC, CVD, and mortality risk in CKD.
IL-10 Gene Polymorphisms and IL-10 Serum Levels in Patients with Multiple Sclerosis in Lithuania
Authors: Masilionyte Ugne , Gedvilaite Greta , Kaikaryte Kriste , Vilkeviciute Alvita , Kriauciuniene Loresa , Glebauskiene Brigita , Balnyte Renata , Liutkeviciene Rasa 
Published: June 2022

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system with features of demyelination and axonal degeneration at a young age. Genetic factors may play an important role in the development of multiple sclerosis. (1) Objective: To investigate IL-10 rs1800871, rs1800872, rs1800896, and IL-10 serum levels in patients with multiple sclerosis. (2) Methods: Our study included patients with multiple sclerosis (n = 127) and healthy volunteers (n = 195). The subjects’ DNA was extracted from peripheral blood leukocytes and genotyped by real-time polymerase chain reaction. The results were analyzed using the program “IBM SPSS Statistics 27.0”. (3) Results: The IL-10 SNPs were analyzed between the MS and control groups; however, no statistically significant results were found. The serum levels of IL-10 in the groups of MS and healthy subjects were not statistically significantly different (median (IQR): 0.828 (1.533) vs. 0.756 (0.528), p = 0.872). (4) Conclusions: IL-10 rs1800871, rs1800872, and rs1800896 and serum IL-10 levels are not likely to be associated with MS development. However, individuals carrying the rare haplotypes of rs1800871, rs1800872, and rs1800896 were associated with increased odds of MS (p = 0.006).
Multicomponent Electrocatalytic Selective Approach to Unsymmetrical Spiro[furo[3,2-c]pyran-2,5′-pyrimidine] Scaffold under a Column Chromatography-Free Protocol at Room Temperature
Authors: Ryzhkova Yuliya E. , Elinson Michail N. , Vereshchagin Anatoly N. , Karpenko Kirill A. , Ryzhkov Fedor V. , Ushakov Ivan E. , Egorov Mikhail P. 
Published: June 2022

Electrochemical synthesis suggested a mild, green and atom-efficient route to interesting and useful molecules, thus avoiding harsh chemical oxidizing and reducing agents used in traditional synthetic methods. Organic electrochemistry offers an excellent alternative to conventional methods of organic synthesis and creates a modern tool for carrying out organic synthesis, including cascade and multicomponent ones. In this research, a novel electrocatalytic multicomponent transformation was found: the electrochemical multicomponent assembly of arylaldehydes, N,N′-dimethylbarbituric acid and 4-hydroxy-6-methyl-2H-pyran-2-one in one pot reaction was carried out in alcohols in an undivided cell in the presence of alkali metal halides with the selective formation of substituted unsymmetrical 1′,3′,6-trimethyl-3-aryl-2′H,3H,4H-spiro[furo[3,2-c]pyran-2,5′-pyrimidine]-2′,4,4′,6′(1′H,3′H)-tetraones in 73–82% yields. This new electrocatalytic process is a selective, facile and efficient way to obtain spiro[furo[3,2-c]pyran-2,5′-pyrimidines]. According to screening molecular docking data using a self-made Python script in Flare, all synthesized compounds may be prominent for different medical applications, such as breast cancer, neurodegenerative diseases and treatments connected with urinary tract, bones and the cardiovascular system.
PENYULUHAN DAN SIMULASI PADA IBU HAMIL DALAM SELF HYPNOSIS MASA PANDEMI COVID 19 WILAYAH KERJA PUSKESMAS HUTABAGINDA TAHUN 2021
Authors: Tiruon Ritonga Paruhum , Hutabarat Naomi Isabella , Rismawaty Nainggolan Dimpu 
Published: June 2022

Self hypnosis adalah menghipnotis diri sendiri yang bagi ibu hamil berfungsi untuk mengurangi stress, kekhawatiran, kecemasan dalam menghadapi persalinan, mengurangi nyeri saat persalinan dan janin di dalam kandungan terhindar dari fetal distres dan meperbaiki letak lintang pada bayi. Tujuan kegiatan ini adalah memberikan pengetahuan ibu hamil untuk dapat melakukan self hypnosis. Manfaat kegiatan ini adalah peingkatan pengetahuan dan keterampilan dalam pemberian self hypnosis.
Sasaran adalah ibu hamil di wilayah kerja Puskesmas Hutabaginda Kecamatan Tarutung Kabupaten Tapanuli Utara. Metode pelaksanaan kegiatan pengabdian adalah melaksanakan pretest dan posttest untuk menilai tingkat kecemasan ibu hamil melalui instrumen Skala HARS, penyuluhan dan simulasi tentang self hypnosis. Hasil yang didapatkan setelah dilakukan self hypnosis selama 1 bulan bahwa ada perbedaan hasil pretest dan posttest yaitu cemas sedang dari 13 orang menjadi 5 orang, Cemas ringan dari 22 orang menjadi 17 orang dan tidak cemas dari 7 orang menjadi 16 orang. Semua ibu hamil berhasil melakukan self hypnosis. Penyuluhan dan diskusi tentang manfaat self hypnosis berjalan lancar dan ibu hamil sangat antusias dan bersemangat melakukan self hypnosis.
Calculation of rainwater harvest in greenhouses for semi-arid and continental climate zones
Authors: Baytorun Abdullah Nafi , Zaimoglu Zeynep , Erkurt Fatma Elçin , Balcı Behzat , Yeşiltaş Hasan Kıvanç 
Published: June 2022

In this study, it is aimed to determine the irrigation water required due to solar radiation in high technology greenhouses where soilless cultivation is carried out according to TS825 standards, and to determine the annual water consumption and storage capacity with the harvested rainwater. As a result of the calculations made for Turkey Mediterranean region, it has been determined that if 90% of the rainfall in the western Mediterranean region is harvested, 72% of the annual water consumption can be met, and 45% in the eastern Mediterranean region. In the inner regions where the terrestrial climate is dominant, 22%–32% of the annual water consumption can be met with 90% of the rain harvested depending on the amount of rainfall. The required storage volume in the western Mediterranean is 0.420 m3 .m-2, while it is 0.096 m3.m-2 in the eastern Mediterranean and 0.044 m3.m-2 in Kırşehir, where the continental climate prevails.
Forecasting the Sum of New College Students with Linear Regression Approach
Authors: Utami Yulia , Vinsensia Desi , Nissa Aura , Sulastri Sulastri 
Published: June 2022

Forecasting models are the result of developments in the field of science and technology that provide convenience in predicting future events. This paper aims to develop a linear regression model to predict the number of new students in the next year. The data to be used in this study is the total of students majoring in informatics engineering and information management during the last 5 years. Based on result obtained the number of student for department of Informatics Engineering is 198 people with a MAPE (Mean Absolute Percentage Error) score of 16.5%, and for the new students department of Informatic Management is 8 people with a MAPE score of 16.1%.