bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving HIV pre-exposure prophylaxis (PrEP) adherence and retention in care: Recommendation development from a national PrEP programme
Authors: MacDonald Jennifer , Estcourt Claudia S , Flowers Paul , Nandwani Rak , Frankis Jamie , Young Ingrid , Clutterbuck Dan , Dalrymple Jenny , McDaid Lisa , Steedman Nicola , Saunders John 
Published: August 2022

AbstractIntroductionHIV pre-exposure prophylaxis (PrEP) is a key component of HIV combination prevention. Effective prevention needs people to adhere to PrEP during periods of risk and remain in care. However, relevant models of care are under-researched. Using data from the first two years of Scotland’s PrEP programme, we explored barriers and facilitators to PrEP adherence and retention in care and systematically developed evidence-based, theoretically-informed recommendations to enhance future adherence and retention.MethodsWe conducted semi-structured interviews and focus groups (09/2018-07/2019) with geographically and demographically diverse patients who were either using/declined/stopped or had been assessed as ineligible for PrEP (n=39), healthcare professionals involved in PrEP provision (n= 54), non-governmental organisation service users (n=9) and staff (n=15) across Scotland. We used thematic analysis to map key barriers and facilitators to priority areas that could enhance adherence and retention in care. Next, we used analytic tools from implementation science (Theoretical Domains Framework, Intervention Functions, Behaviour Change Technique Taxonomy, APEASE criteria) and expert opinion to systematically generate recommendations to enhance future PrEP adherence and retention in care.ResultsBarriers and facilitators to adherence and retention in care were diverse and multi-layered. Barriers included perceived complexity of event-based dosing, the tendency for users to stop PrEP before seeking professional support, troublesome side-effects, limited flexibility in the settings, timings, and nature of appointments for follow up, enduring PrEP-related stigma and emerging stigmas around not using PrEP. Facilitators included flexible appointment scheduling, reminders, and processes to follow up non-attenders. We generated 25 wide-ranging but specific recommendations for key stakeholders, for example, emphasising the benefits of PrEP reviews and providing appointments flexibly within individualised PrEP care; using clinic systems to remind/recall PrEP users for review; supporting PrEP conversations among sexual partners; clear guidance on event-based dosing; encouraging/commitment to good PrEP citizenship; and detailed discussion on managing side-effects and care/coping planning activities.ConclusionsPrEP adherence and retention in care is challenging for many people. Such challenges reduce the benefits of PrEP at individual and population levels. Our findings identify and provide solutions to where and how collaborative interventions across public health, clinical, and community practice could address these challenges.
“It’s a helluva journey”: A qualitative study of patient and clinician experiences of nausea and vomiting syndromes and functional dyspepsia
Authors: Sebaratnam Gabrielle , Law Mikaela , Broadbent Elizabeth , Gharibans Armen A , Andrews Christopher N , Daker Charlotte , O’Grady Greg , Calder Stefan , Keane Celia 
Published: August 2022

AbstractBackgroundChronic gastroduodenal disorders including chronic nausea and vomiting syndrome, gastroparesis, and functional dyspepsia, are challenging to diagnose and manage. The diagnostic and treatment pathways for these disorders are complex, costly and overlap substantially; however, experiences of this pathway have not been thoroughly investigated. This study therefore aimed to explore clinician and patient perspectives on the current clinical pathway.MethodsSemi-structured interviews were conducted between June 2020 and June 2022 with 11 patients with chronic nausea and vomiting syndrome alone or with functional dyspepsia (based on Rome IV criteria) and nine gastroenterologists who treat these conditions. Interviews were recorded, transcribed, and thematically analyzed using an iterative, inductive approach.ResultsFive key patient themes were identified: (1) the impacts of their chronic gastroduodenal symptoms, (2) the complexity of the clinical journey, (3) their interactions with healthcare providers, (4) the need for advocacy, and (5) their experience of treatments. Five key clinician themes were also identified: (1) these conditions were seen as clinically complex, (2) there is an uncertain and variable clinical pathway, (3) the nuance of investigations, (4) these conditions were difficult to therapeutically manage, and (5) there are barriers to developing a therapeutic relationship.ConclusionsFindings indicate that both patients and clinicians are dissatisfied with the current clinical care pathways for nausea and vomiting syndromes and functional dyspepsia. Recommendations included the development of more clinically relevant and discriminant tests, standardization of the diagnostic journey, and the adoption of a multidisciplinary approach to diagnosis and treatment.
Cytoplasmic contractile injection systems mediate cell death inStreptomyces
Authors: Casu Bastien , Sallmen Joseph W. , Schlimpert Susan , Pilhofer Martin 
Published: August 2022

AbstractContractile injection systems (CISs) are bacteriophage tail-like structures that mediate bacterial cell-cell interactions. While CISs are highly abundant across diverse bacterial phyla, representative gene clusters in Gram-positive organisms remain poorly studied.Here we characterize a CIS in the Gram-positive multicellular model organismStreptomyces coelicolorand show, that in contrast to most other CISs,S. coelicolorCIS (CISSc) mediate cell death in response to stress and impact cellular development. CISScare expressed in the cytoplasm of vegetative hyphae and not released into the medium. Our cryo-electron microscopy structure enabled the engineering of non-contractile and fluorescently tagged CISScassemblies. Cryo-electron tomography showed that CISSccontraction is linked to reduced cellular integrity. Fluorescence light microscopy furthermore revealed that CISSccontraction mediates cell death upon encountering different types of stress. Finally, the absence of functional CISSchad an impact on hyphal differentiation and secondary metabolite production.Our results provide new functional insights into CISs in Gram-positive organisms and a framework for studying novel intracellular roles, including regulated cell death and life cycle progression in multicellular bacteria.
Rapid proteostasis controls monolayer integrity of quiescent endothelium
Authors: Podieh Fabienne , Wensveen Roos , Overboom Max C. , Abbas Lotte , Majolée Jisca , Hordijk Peter L. 
Published: August 2022

SummaryEndothelial monolayer permeability is regulated by actin dynamics and vesicular traffic. Recently, ubiquitination was also implicated in the integrity of quiescent endothelium, as it differentially controls the localization and stability of adhesion- and signaling proteins. We found that inhibition of E1 ubiquitin ligases induces a rapid, reversible loss of integrity in quiescent, primary human endothelial monolayers, accompanied by increased F-actin stress fibers and the formation of intercellular gaps. Concomitantly, total protein and activity of the actin-regulating GTPase RhoB, but not its close homologue RhoA, increase ∼10-fold in 5-8 h. The depletion of RhoB, but not of RhoA, the inhibition of actin contractility and the inhibition of protein synthesis all significantly rescue the loss of cell-cell contact induced by E1 ligase inhibition. Our data suggest that in quiescent human endothelial cells, the continuous and fast turnover of short-lived proteins that negatively regulate cell-cell contact, is essential to preserve monolayer integrity.
Leishmania mexicanaPromotes Pain-reducing Metabolomic Reprogramming In Cutaneous Lesions
Authors: Volpedo Greta , Oljuskin Timur , Cox Blake , Mercado Yulian , Askwith Candice , Azodi Nazli , Gannavaram Sreenivas , Nakhasi Hira L. , Satoskar Abhay R. 
Published: August 2022

ABSTRACTCutaneous leishmaniasis (CL) is characterized by extensive skin lesions associated with an aggressive inflammatory reaction. Despite the extensive inflammation, CL lesions are usually painless, indicating thatLeishmaniainfection may trigger anti-nociceptive activities in the infected tissues. To this date, the molecular mechanisms responsible for this clinical phenomenon have not been identified. Through an untargeted metabolomic analysis by mass spectrometry, we found enriched anti-nociceptive metabolic pathways in mice infected withLeishmania(L.)mexicana.In particular, endogenous purines were elevated at the lesion site during chronic infection, as well asin vitroin infected macrophages, compared to non-infected mice. These purines have known anti-inflammatory and analgesic properties by acting through adenosine receptors and inhibiting transient receptor potential channels of the vanilloid subtype 1 (TRPV1). Additionally, purine metabolites can promote interleukin (IL)-10 production, with a subsequent decrease in inflammation and pain sensitivity. We also found arachidonic acid metabolism enriched in the ear lesions compared to the non-infected controls. Arachidonic acid is a metabolite of anandamide (AEA) and 2-arachidonoylglycerol (2-AG). These endocannabinoids act on cannabinoid receptors 1 and 2 and TRPV1 channels to exert anti-inflammatory and analgesic effects. Our study provides the first evidence of metabolic pathways upregulated duringL. mexicanainfection that may mediate anti-nociceptive effects experienced by CL patients and identifies macrophages as a source of these metabolites.Graphical abstractL. mexicanainfection promotes the production of purines, as well as endocannabinoid mediators, which could act on different channels of dorsal root ganglia neuron to inhibit nociception.
Cellular profiling of a recently-evolved social behavior
Authors: Johnson Zachary V. , Hegarty Brianna E. , Gruenhagen George W. , Lancaster Tucker J. , McGrath Patrick T. , Streelman Jeffrey T. 
Published: August 2022

ABSTRACTSocial behaviors are essential for survival and reproduction and vary within and among species. We integrate single nucleus RNA-sequencing (snRNA-seq), comparative genomics, and automated behavior analysis to investigate a recently-evolved social “bower building” behavior in Lake Malawi cichlid fishes. We functionally profile telencephalic nuclei matched to 38 paired behaving/control individuals. Our data suggest bower behavior has evolved in part through divergence in a gene module selectively expressed in a subpopulation of glia lining the pallium. Downregulation of the module is associated with glial departure from quiescence and rebalancing of neuronal subpopulation proportions in the putative homologue of the hippocampus. We show further evidence that behavior-associated excitation of neuronal populations that project to the putative hippocampus mediate glial function and rebalancing. Our work suggests that bower behavior has evolved through changes in glia and region-specific neurogenesis, and more broadly shows how snRNA-seq can generate insight into uncharted behaviors and species.
Business model design and mass customization capability: is supply chain integration a missing link?
Authors: Cheng Jia , Zhao Songzheng , Feng Taiwen , Sheng Hongyan 
Published: August 2022

PurposeThis study aims to examine the impacts of the novelty-centered business model design (NBMD) and efficiency-centered business model design (EBMD) on mass customization capability (MCC), as well as the mediating role of supply chain integration.Design/methodology/approachUsing survey data from 277 Chinese manufacturing firms, we test the hypothesized relationships by conducting structural equation modeling.FindingsThe results indicate that both NBMD and EBMD have significantly positive impacts on product-oriented MCC and service-oriented MCC. In addition, three dimensions of supply chain integration play different mediating roles in the relationship between BMD and MCC. Specifically, relational integration partially mediates the impacts of NBMD and EBMD on service-oriented MCC, information integration partially mediates the impact of NBMD on product-oriented MCC and service-oriented MCC and operational integration partially mediates the impact of NBMD and EBMD on product-oriented MCC.Originality/valueThis study opens the “black box” in the relationship between business model design and MCC, which offers insights on the complex process of supply chain integration and considers business ecosystem for operational performance.
Predicting Romanian tourism e-WOM intentions using value and personality theories
Authors: Baber Hasnan , Pană Marius-Cristian , Fanea-Ivanovici Mina 
Published: August 2022

PurposeThe tourism industry has a huge potential, and the return to its state prior to the COVID-19 pandemic is a large stake for any economy. Tourism e-WOM is an important contributor towards attracting visitors and choosing tourism destinations. Drawing from the value and personality theories, the aim of this study is to investigate the factors determining e-WOM intentions in tourism.Design/methodology/approachBased on a sample of 469 responses collected online from a random sample of Romanians and using partial least squares structural equation modelling, the authors hereby analyze the influence of economic, emotional, social and altruistic values on e-WOM intentions, and the moderating effects of the big five personality traits (openness, conscientiousness, extraversion, agreeableness and neuroticism) on this relationship.FindingsThe results of the study revealed that except economic value, all the exogenous (emotional, social and altruistic) values have a positive influence on the e-WOM intentions. However, the study further suggested that the big five personality traits do not moderate the relationship between the value factors and e-WOM intentions.Research limitations/implicationsThe influence of perceived value will be useful for building a stout marketing strategy and to describe e-WOM behaviour among Romanians.Practical implicationsTourism service providers can promote their services by stressing the various types of value bestowed by their offers.Originality/valueAlthough inspired by existing research, the current study is original in that the model used has not yet been proposed before, all the more so for the particular case of the tourism industry.
Obesity, family units and social marketing intervention: evidence from Nigeria
Authors: Nwoba Arinze Christian , Mogaji Emmanuel , Zahoor Nadia , Donbesuur Francis , Alam Gazi Mahabubul 
Published: August 2022

PurposeBuilding on the social marketing theory, this study aims to examine the relationship between family units and obesity in Nigeria; and the social marketing interventions used to reduce and prevent obesity in the Nigerian society.Design/methodology/approachThis study adopted a semi-structured interview research design with 42 obese individuals in Nigeria.FindingsThe study findings show that the family unit an individual grows up in influences their consumption behaviour, which drives their obesity. The findings reveal that obese Nigerian citizens are willing to live a healthier lifestyle due to the direct and indirect medical costs associated with obesity. Furthermore, the findings disclose the social marketing interventions – local celebrity endorsements, healthy lifestyle promotions, reduced gym membership and affordable access to healthy foods and services – used to prevent and reduce the rising obesity rates in the Nigerian society.Research limitations/implicationsThe findings have important theoretical implication given the focus on consumption behaviour and obesity.Practical implicationsThe study findings provide an avenue to guide government officials, policymakers and social marketers in shaping their public policy and social marketing interventions to encourage healthier consumption and lifestyle behaviours among families and individuals in the Nigerian society.Originality/valueTo the best of the authors’ knowledge, this is the first research study to investigate how family units in the emerging market of sub-Saharan Africa drive obesity and the social marketing interventions used to reduce and prevent obesity. Theoretical and practical implications are discussed.
Effect of far-infrared fabrics on proliferation and invasion of breast cancer cells
Authors: Mu Yifei , Jin Zimin , Yan Yuxiu , Tao Jianwei 
Published: August 2022

PurposeIn order to study whether far-infrared fabrics can be used as a garment for breast cancer patients, or as an adjuvant rehabilitation underwear for breast cancer patients after postoperative radiotherapy and chemotherapy, to eliminate tissue edema. To explore the effect of different far-infrared fabrics on the proliferation and invasion of breast cancer cells as a basic in vitro study.Design/methodology/approachSix kinds of fabrics of the same specification with different far-infrared nanoparticles were selected. MCF7 and Bcap37 breast cancer cells were used to study the effect of far-infrared fabrics on cell proliferation and invasion. Six kinds of far-infrared fabrics were used to culture breast cancer cells and explore their effects on breast cancer cell growth and the difference between different far-infrared fabrics.FindingsIt is found that the far-infrared emissivity of six kinds of fabrics are different, among which tea carbon fabric is the highest, followed by volcanic fabric, graphene fabric and biomass graphene fabric are the lowest. The results show that the far-infrared fabrics can significantly inhibit the proliferation and invasion of breast cancer cells, the higher the far-infrared emissivity is, and the longer the time of far-infrared radiation, the more significant the inhibition effect is.Originality/valueFar-infrared fabrics can inhibit proliferation and invasion of breast cancer cells in vitro. Therefore, far-infrared fabrics can be used for adjuvant rehabilitation of breast cancer patients. This conclusion provides a basis for the application of far-infrared functional fabrics in the medical field. This conclusion provides a basis for the application of far-infrared functional fabrics in medical field.
An exposition of the constructive research approach: a tactical treatise for addressing methodological and practical issues in organisational research
Authors: Jones Ollie , Gold Jeff , Claxton Julia 
Published: August 2022

Purpose
This paper aims to provide an exposition of the constructive research approach (CRA) to show the potential utility of CRA in transcending or mitigating the methodological and practical issues involved in researching organisations.

Design/methodology/approach
The paper is a literature review, and resulting thematic discussion of methodological and practical issues involves in action research (AR) in organisations through the lens of the CRA approach.

Findings
The paper identifies that CRA has benefits in orientation to a practical outcome grounded in a theoretical domain but with leeway to facilitate creativity, which can also potentially improve the quality of the collaborative relationships. The centrality of the construction within the method provides a “vantage point” to manage the emic (inside) and etic (outside) positionality concerns of action researchers working within organisational settings.

Practical implications
CRA has multiple practical benefits for action researchers and their collaborators in terms of time, risk and collaborative commitment.

Originality/value
The paper develops a useful tactical framework for discussing the practical and methodological issues when considering AR in organisations and highlights how CRA can be used in wider organisational scholarship outside its roots in management accounting.
A review of integrated thinking research in developed and developing economies
Authors: Ecim Dusan , Maroun Warren 
Published: August 2022

PurposeThis paper assesses the state and development of integrated thinking research in selected developed and developing economies by providing a “stock taking” of the literature included in the Scopus Database based on filtered criteria.Design/methodology/approachA search was performed on the Scopus Database and all sources containing “integrated thinking” in the title, abstract, or keywords were extracted. A total of 98 sources from 2011–2021 are analysed. These are coded with the support of a content analysis and a bibliometric analysis to determine research objectives and methods, affiliations, the locus of enquiry and epistemological perspectives.FindingsThere is a steady increase in attention devoted to integrated thinking research. The earliest studies were concerned primarily with the type of extra-financial information being included in an organisation's reports. This has given way to studies concerned with the operationalisation of integrated thinking. The current research has predominantly focussed on non-positivist epistemologies at the macro- and meso-levels with limited research undertaken at organisational levels.Practical implicationsResearch on integrated thinking is particularly relevant in the context of developing economies where integrated thinking can be used as a means to value-creation, sustainable development and stakeholder inclusivity.Social implicationsThis paper provides a useful reference for practitioners, academics and journal editors interested in the development of integrated thinking research.Originality/valueThis paper highlights the need for more active research on integrated thinking and reporting in developing economies because much of what has been published to date comes from developed nations. This paper provides an overview of the state of integrated thinking research and presents important areas for future research.
A cloud-based collaborative ecosystem for the automation of BIM execution plan (BEP)
Authors: Abbas Muhammad Azeem , Ajayi Saheed O. , Oyegoke Adekunle Sabitu , Alaka Hafiz 
Published: August 2022

Purpose
Master information delivery plan (MIDP) is a key requirement for building information modelling (BIM) execution plan (BEP) that enlists all information deliverables in BIM-based project, containing information about what would be prepared, when, by who, as well as the procedures and protocols to be used. In a well-conceived BEP, the MIDP facilitates collaboration among stakeholders. However, current approaches to generating MIDP are manual, making it tedious, error-prone and inconsistent, thereby limiting some expected benefits of BIM implementation. The purpose of this study is to automate the MIDP and demonstrate a collaborative BIM system that overcomes the problems associated with the traditional approach.

Design/methodology/approach
A BIM cloud-based system (named Auto-BIMApp) involving naming that automated MIDP generation is presented. A participatory action research methodology involving academia and industry stakeholders is followed to design and validate the Auto-BIMApp.

Findings
A mixed-method experiment is conducted to compare the proposed automated generation of MIDP using Auto-BIMApp with the traditional practice of using spreadsheets. The quantitative results show over 500% increased work efficiency, with improved and error-free collaboration among team members through Auto-BIMApp. Moreover, the responses from the participants using Auto-BIMApp during the experiment shows positive feedback in term of ease of use and automated functionalities of the Auto-BIMApp.

Originality/value
The replacement of traditional practices to a complete automated collaborative system for the generation of MIDP, with substantial productivity improvement, brings novelty to the present research. The Auto-BIMApp involve multidimensional information, multiple platforms, multiple types and levels of users, and generates three different representations of MIDP.