bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comandos de produção textual:
Authors: Bedin Karime Torres , Costa-H¨übes Terezinha da Conceição 
Published: December 2022

RESUMO: Compreender a linguagem no ensino da produção de texto como forma de interação significa assumir uma conduta discursiva, que a considera na relação entre sujeitos situados sócio-historicamente. É essa compreensão que subjaz o Currículo dos Anos Iniciais (AMOP, 2007, 2015, 2020), ao orientar o ensino de Língua Portuguesa (LP), a partir do qual questionamo-nos: que elementos de uma proposta de escrita como forma de interação estão sendo garantidos em comandos escritos de produção textual? Assim, objetivamos, neste artigo, refletir sobre a escrita como forma de interação e sobre suas implicações na condução da atividade de produção textual. Para isso, olhamos para comandos de produção de texto elaborados por professores do 5º ano do Ensino Fundamental - Anos Iniciais, da região Oeste do Paraná, amparadas em autores como Geraldi (1997), Menegassi (2004), Costa-Hübes (2012), sem perder de vista escritos do Círculo de Bakhtin. As análises nos mostram que ainda são poucos os elementos da interação contemplados pelos professores na elaboração de comandos de produção de texto que antecedem a escrita do aluno.

PALAVRAS-CHAVE: Escrita como forma de interação; Pressupostos curriculares; Comandos de produção de texto; Análise Reflexiva.
Longitudinal in vivo biodistribution of nano and micro sized hydroxyapatite particles implanted in a bone defect
Authors: Liu Yang , Sebastian Sujeesh , Huang Jintian , Corbascio Tova , Engellau Jacob , Lidgren Lars , Tägil Magnus , Raina Deepak Bushan 
Published: December 2022

Hydroxyapatite (HA) has been widely used as a bone substitute and more recently as a carrier for local delivery of bone targeted drugs. Majority of the approved HA based biomaterials and drug carriers comprise of micrometer sized particulate HA (mHA) or granules and can therefore only be used for extracellular drug release. This shortcoming could be overcome with the use of cell penetrating HA nanoparticles (nHA) but a major concern with the clinical use of nHA is the lack of data on its in vivo biodistribution after implantation. In this study, we aimed to study the in vivo biodistribution of locally implanted nHA in a clinically relevant tibial void in rats and compare it with mHA or a combination of mHA and nHA. To enable in vivo tracking, HA particles were first labelled with 14C-zoledronic acid (14C-ZA), known to have a high binding affinity to HA. The labelled particles were then implanted in the animals and the radioactivity in the proximal tibia and vital organs was detected at various time points (Day 1, 7 and 28) post-implantation using scintillation counting. The local distribution of the particles in the bone was studied with micro-CT. We found that majority (&gt;99.9%) of the implanted HA particles, irrespective of the size, stayed locally at the implantation site even after 28 days and the findings were confirmed using micro-CT. Less than 0.1% radioactivity was observed in the kidney and the spleen at later time points of day 7 and 28. No pathological changes in any of the vital organs could be observed histologically. This is the first longitudinal in vivo HA biodistribution study showing that the local implantation of nHA particles in bone is safe and that nHA could potentially be used for localized drug delivery.
Evaluation of a rapid susceptibility test of polymyxin B by MALDI-TOF
Authors: Barth Patricia Orlandi , Volpato Fabiana Caroline Zempulski , Moreira Natália Kehl , Wink Priscila Lamb , de Souza Ândrea Celestino , Barth Afonso Luís 
Published: December 2022

IntroductionInfections caused by multidrug-resistant microorganisms have become increasingly common in hospital environments around the world. Gram-negative bacilli stands out among multidrug-resistant bacteria mostly due to the production of carbapenemase enzymes which lead to resistance to most β-lactam antibiotics including the carbapenems. As a consequence, polymyxins have been reintroduced in the clinic as a last resort to treat infections caused by Gram-negative bacilli resistant to carbapenems. However, the only reliable method to evaluate the susceptibility to polymyxins is the broth microdilution, a laborious and time-consuming technique. Among infections caused by multidrug-resistant bacteria, bloodstream infections are the most worrisome as they can lead to sepsis and septic shock with high mortality rates.ObjectiveConsidering the severity of sepsis and the need for a treatment guided for the susceptibility test in vitro, this work aimed to evaluate a rapid method of polymyxins susceptibility either from colonies grown on agar or directly from positive blood culture bottles using the technology of MALDI-TOF.MethodsThe method was based on the “direct on target microdroplets growth assay” (DOT-MGA) originally developed by Idelevich and collaborators with some modifications (Adapted DOT-MGA). Isolates of Enterobacterales and non-fermenting Gram-negative bacilli resistant to carbapenems were obtained from patients attending a tertiary care hospital in southern Brazil and tested as follows: 122 isolates from colonies grown on agar plates and 117 isolates directly from spiked positive blood cultures.ResultsThe adapted DOT-MGA presented 95 and 100% of categorical agreement considering the colonies grown on agar plates and directly from positive blood cultures, respectively.DiscussionThe adapted DOT-MGA test proved to be a reliable technique to evaluate the susceptibility to polymyxins to be used in microbiology laboratories with the MALDI-TOF equipment.
Tuberculous pleural effusion prediction using ant colony optimizer with grade-based search assisted support vector machine
Authors: Li Chengye , Hou Lingxian , Pan Jingye , Chen Huiling , Cai Xueding , Liang Guoxi 
Published: December 2022

IntroductionAlthough tuberculous pleural effusion (TBPE) is simply an inflammatory response of the pleura caused by tuberculosis infection, it can lead to pleural adhesions and cause sequelae of pleural thickening, which may severely affect the mobility of the chest cavity.MethodsIn this study, we propose bGACO-SVM, a model with good diagnostic power, for the adjunctive diagnosis of TBPE. The model is based on an enhanced continuous ant colony optimization (ACOR) with grade-based search technique (GACO) and support vector machine (SVM) for wrapped feature selection. In GACO, grade-based search greatly improves the convergence performance of the algorithm and the ability to avoid getting trapped in local optimization, which improves the classification capability of bGACO-SVM.ResultsTo test the performance of GACO, this work conducts comparative experiments between GACO and nine basic algorithms and nine state-of-the-art variants as well. Although the proposed GACO does not offer much advantage in terms of time complexity, the experimental results strongly demonstrate the core advantages of GACO. The accuracy of bGACO-predictive SVM was evaluated using existing datasets from the UCI and TBPE datasets.DiscussionIn the TBPE dataset trial, 147 TBPE patients were evaluated using the created bGACO-SVM model, showing that the bGACO-SVM method is an effective technique for accurately predicting TBPE.
Reconstruction after resection of C2 vertebral tumors: A comparative study of 3D-printed vertebral body versus titanium mesh
Authors: Hu Panpan , Du Suiyong , Wei Feng , Zhai Shuheng , Zhou Hua , Liu Xiaoguang , Liu Zhongjun 
Published: December 2022

BackgroundSurgical resection of C2 vertebral tumors is challenging owing to the complex anatomy of C2 vertebrae and the challenges to surgical exposure. Various surgical approaches are available, but some are associated with excessively high risks of complications. An additional challenge is reconstruction of the upper cervical spine following surgery. In the last decade, additive-manufacturing personalized artificial vertebral bodies (AVBs) have been introduced for the repair of large, irregular bony defects; however, their use and efficacy in upper cervical surgery have not been well addressed. Therefore, in this study, we compared instrumented fixation status between patients who underwent conventional titanium mesh reconstruction and those who underwent the same resection but with personalized AVBs.MethodsWe performed a retrospective comparative study and recruited a single-institution cohort of patients with C2 vertebral tumors. Clinical data and imaging findings were reviewed. Through data processing and comparative analysis, we described and discussed the feasibility and safety of surgical resection and the outcomes of hardware implants. The primary outcome of this study was instrumented fixation status.ResultsThe 31 recruited patients were divided into two groups. There were 13 patients in group A who underwent conventional titanium mesh reconstruction and 18 group B patients who underwent personalized AVBs. All patients underwent staged posterior and anterior surgical procedures. In the cohort, 9.7% achieved total en bloc resection of the tumor, while gross total resection was achieved in the remaining 90.3%. The perioperative complication and mortality rates were 45.2% and 6.5%, respectively. The occurrence of perioperative complications was related to the choice of anterior approach (p &lt; 0.05). Group A had a higher complication rate than group B (p &lt; 0.05). Four patients (4/13, 30.8%) developed hardware problems during the follow-up period; however, this rate was marginally higher than that of group B (1/18, 5.6%).ConclusionsTotal resection of C2 vertebral tumors was associated with a high risk of perioperative complications. The staged posterior and retropharyngeal approaches are better surgical strategies for C2 tumors. Personalized AVBs can provide a reliable reconstruction outcome, yet minor pitfalls remain that call for further modification.
Measuring the impact of the COVID-19 epidemic on university resumption and suggestions for countermeasures
Authors: Yin Shi , Ma Lijun , Dong Tong , Wang Ying 
Published: December 2022

BackgroundDuring the COVID-19 pandemic, universities around the world had to find a balance between the need to resume classes and prevent the spread of the virus by ensuring the health of students. The purpose of our study was to effectively assess the overall risk of universities reopening during the COVID-19 epidemic.Design and methodsUsing the pressure–state–response model, we designed a risk evaluation method from a disaster management perspective. First, we performed a literature review to find the main factors affecting the virus spread. Second, we used the pressure–state–response to represent how the considered hazards acts and interacts before grouping them as disaster and vulnerability factors. Third, we assigned to all factors a risk function ranging from 1 to 4. Fourth, we modeled the risk indexes of disaster and of system vulnerability through simple and appropriate weights and combined them in an overall risk for the university resumption. Finally, we showed how the method works by evaluating the reopening of the Hebei Province University in 2022 and highlighted the resulting advice for reducing related risks.ResultsOur model included 20 risk factors, six representing exogenous hazards (disaster factors) that university can only monitor and 14 related to system vulnerability that can also control. Disaster factors included epidemic risk level of students' residence and the school's location, means of transportation back to school, size of the university population, the number of migrants on and off campus and express carrier infection. Vulnerability factors included student behaviors, routine campus activities and all the other actions the university can take to control the virus spread. The university of Baoding city (Hebei Province) showed a disaster risk of 1.880 and a vulnerability of 1.666 which combined provided a low risk of school resumption.ConclusionOur study judged the risks involved in resuming school and put forward specific countermeasures for reducing the risk levels. This not only protects public health security but also has some practical implications for improving the evaluation and rational decision-making abilities of all parties.
Predictors of fear of diabetes progression: A multi-center cross-sectional study for patients self-management and healthcare professions education
Authors: Wang Yanhao , Yu Qiuhua , Zeng Zihuan , Yuan Ruizhu , Wang Ruiding , Chen Jianli , Zhou Hengyu , Tang Jiao 
Published: December 2022

ObjectiveExcessive fear of progression can affect the mental health, social function, and wellbeing of patients with chronic diseases. This study investigated the fear of progression (FoP) and the socio-demographic and clinical predictors among patients with type 2 diabetes mellitus (T2DM).MethodThe present study is a multi-center cross-sectional study. Inpatients with T2DM were recruited by a multi-stage convenience sampling method from the department of endocrinology in 5 tertiary hospitals in Southwest China. 459 T2DM patients were consecutively enrolled. Socio-demographic, clinical data, and answers to the fear of progression questionnaire (FoP-Q) were collected.Results385 patients with complete data were eligible. The average score of FoP-Q-SF was 26.84 and 23.1% of patients reached the dysfunctional fear of progression criterion. The greatest fears were worrying about “disease progression,” “the adverse reactions of medication,” and “relying on strangers for activities of daily living.” Health education (P &lt; 0.001), age (P = 0.002), hypoglycemia history (P = 0.006), employment status (P = 0.025) and duration since being diagnosed with type 2 diabetes mellitus (P = 0.032) were the related factors of fear of progression.ConclusionEarly assessment of the fear of progression was imperative to identify dysfunctional fear of progression in patients with type 2 diabetes mellitus. Meanwhile, the meaning of these predictors for strengthening healthcare professions education and patients self-management might help healthcare givers timely perform related interventions and help patients reduce their fear of progression thus actively cooperate with T2DM treatments.
Impact of Coxiella burnetii vaccination on humoral immune response, vaginal shedding, and lamb mortality in naturally pre-infected sheep
Authors: Bauer Benjamin Ulrich , Schoneberg Clara , Herms Thea Louise , Kleinschmidt Sven , Runge Martin , Ganter Martin 
Published: December 2022

IntroductionSheep are considered to be one of the main reservoirs for Coxiella burnetii, a gram-negative bacterium with high zoonotic potential. Infected sheep shed tremendous amounts of the pathogen through birth products which caused human Q fever epidemics in several countries. Information about the impact of an inactivated C. burnetii Phase I vaccine on humoral immune response, vaginal shedding, and lamb mortality in naturally pre-infected sheep is scarce.MethodsTwo identically managed and naturally C. burnetii-infected sheep flocks were examined for two lambing seasons (2019 and 2020). One flock (VAC) received a primary vaccination against Q fever before mating and the second flock served as control (CTR). In each flock, one cohort of 100 ewes was included in follow-up investigations. Serum samples at eight different sampling dates were analyzed by C. burnetii phase-specific ELISAs to differentiate between the IgG Phase I and II responses. Vaginal swabs were collected within three days after parturition and examined by a C. burnetii real-time PCR (IS1111). Lamb losses were recorded to calculate lamb mortality parameters.ResultsAfter primary vaccination, almost all animals from cohort VAC showed a high IgG Phase I response up until the end of the study period. In cohort CTR, the seropositivity rate varied from 35.1% to 66.3%, and the Phase I and Phase II pattern showed an undulating trend with higher IgG Phase II activity during both lambing seasons. The number of vaginal shedders was significantly reduced in cohort VAC compared to cohort CTR during the lambing season in 2019 (p &lt; 0.0167). There was no significant difference of vaginal shedders in 2020. The total lamb losses were low in both cohorts during the two investigated lambing seasons (VAC 2019: 6.8%, 2020: 3.2%; CTR 2019: 1.4%, 2020: 2.7%).DiscussionNeither the C. burnetii vaccine nor the C. burnetii infection seem to have an impact on lamb mortality. Taken together, the inactivated C. burnetii Phase I vaccine induced a strong IgG Phase I antibody response in naturally pre-infected sheep. It might also reduce vaginal shedding in the short term but seems to have little beneficial impact on lamb mortality.
Biology, Genetic Diversity, and Ecology of Nitzschia acidoclinata Lange-Bertalot (Bacillariophyta)
Authors: Bagmet Veronika B. , Abdullin Shamil R. , Nikulin Arthur Yu. , Nikulin Vyacheslav Yu. , Gontcharov Andrey A. 
Published: December 2022

The diatom Nitzschia acidoclinata is a widespread eurybiontic alga. There is little information on its life cycle properties and cardinal points. To fill this gap, we analyzed six N. acidoclinata clones from a range of habitats in Asiatic Russia regarding their genetic diversity, morphology, morphometry, geography, and ecology. A comparison of 15 N. acidoclinata rbcL sequences sampled across its relatively wide distribution area and contrasting habitats revealed no distinct genotypes in the species. We demonstrated that the valve morphology, their length, and the sexual activity of the investigated clones varied depending on the phase of their life cycle. In this species, abrupt size reduction was observed. It was revealed that N. acidoclinata reproduced by pedogamy, and its auxosporulation was season-dependent and observed in spring and autumn only. The mating activity in our clones was detected only when the cell size was reduced to 9–22 µm in length. The available data on sexual reproduction in the genus Nitzschia suggest that neither clades nor subclades comprise pedogamous or anisogamous taxa at the same time. However, isogamy could occur in the same clade with either pedogamy or anisogamy. These data provide a fundamental basis for the development of N. acidoclinata mass cultivation and long-term maintenance in culture technologies.
Cryptococcus neoformans Causing Meningoencephalitis in Adults and a Child from Lima, Peru: Genotypic Diversity and Antifungal Susceptibility
Authors: Firacative Carolina , Zuluaga-Puerto Natalia , Guevara José 
Published: December 2022

Cryptococcosis, caused predominantly by Cryptococcus neoformans, is a potentially fatal, opportunistic infection that commonly affects the central nervous system of immunocompromised patients. Globally, this mycosis is responsible for almost 20% of AIDS-related deaths, and in countries like Peru, its incidence remains high, mostly due to the annual increase in new cases of HIV infection. This study aimed to establish the genotypic diversity and antifungal susceptibility of C. neoformans isolates causing meningoencephalitis in 25 adults and a 9-year-old girl with HIV and other risk factors from Lima, Peru. To identify the genotype of the isolates, multilocus sequence typing was applied, and to establish the susceptibility of the isolates to six antifungals, a YeastOne® broth microdilution was used. From the isolates, 19 were identified as molecular type VNI, and seven as VNII, grouped in eight and three sequence types, respectively, which shows that the studied population was highly diverse. Most isolates were susceptible to all antifungals tested. However, VNI isolates were less susceptible to fluconazole, itraconazole and voriconazole than VNII isolates (p < 0.05). This study contributes data on the molecular epidemiology and the antifungal susceptibility profile of the most common etiological agent of cryptococcosis, highlighting a pediatric case, something which is rare among cryptococcal infection.
Genome-Wide Identification and Characterization of TCP Gene Family Members in Melastoma candidum
Authors: Li Hui , Wen Xiaoxia , Huang Xiong , Wei Mingke , Chen Hongpeng , Yu Yixun , Dai Seping 
Published: December 2022

It has been confirmed that the plant-specific Teosinte-branched 1/Cycloidea/Proliferating (TCP) gene family plays a pivotal role during plant growth and development. M. candidum is a native ornamental species and has a wide range of pharmacodynamic effects. However, there is still a lack of research on TCP’s role in controlling M. candidum’s development, abiotic stress responses and hormone metabolism. A comprehensive description of the TCP gene family in M. candidum is urgently needed. In this study, we used the HMMER search method in conjunction with the BLASTp method to identify the members of the TCP gene family, and a total of 35 TCP genes were identified. A domain analysis further confirmed that all 35 TCPs contained a TCP superfamily, a characteristic involved in dimerization and DNA binding that can be found in most genes from this gene family, suggesting that our identification was effective. As a result of the domain conservation analysis, the 35 TCP genes could be classified into two classes, TCP-P and TCP-C, based on the conservative regions of 55 and 59 amino acids, respectively. Gene-duplication analysis revealed that most TCP genes were present in duplication events that eventually led to TCP gene expansion in M. candidum. All the detected gene pairs had a Ka/Ks value of less than one, suggesting that purification selection is the most important factor that influences the evolution of TCP genes. Phylogenetic analysis of three species displayed the evolutionary relationship of TCP genes across different species and further confirmed our results. The real-time quantitative PCR (qRT-PCR) results showed that McTCP2a, McTCP7a, McTCP10, McTCP11, McTCP12a, McTCP13, McTCP16, McTCP17, McTCP18, McTCP20 and McTCP21 may be involved in leaf development; McTCP4a, McTCP1, McTCP14, McTCP17, McTCP18, McTCP20, McTCP22 and McTCP24 may be involved in flower development; and McTCP2a, McTCP3, McTCP5a, McTCP6, McTCP7a, McTCP9, McTCP11, McTCP14 and McTCP16 may be involved in seed development. Our results dissect the TCP gene family across the genome of M. candidum and provide valuable information for exploring TCP genes to promote molecular breeding and property improvement of M. candidum in the future.
An experimental Study Investigating the Effects on Brassica oleracea: Estuarine Seaweeds as Biostimulants in Seedling Development?
Authors: Mendes Madalena , Pacheco Diana , Cotas João , Bahcevandziev Kiril , Pereira Leonel 
Published: December 2022

Estuarine eutrophication due to the nutrient run-off from the agricultural fields encourages the establishment of several opportunistic seaweeds. These fast-growing seaweeds, considered an untapped resource, with a circular economy approach, can be employed as soil plant fertilizer. In agriculture, there is a global trend toward shifting from chemical-based conventional farming to sustainable agriculture. In this context, this study aimed to understand the biostimulant potential of the aqueous extracts from seaweeds harvested in Mondego estuary located on the Atlantic coast of Portugal, namely Ulva lactuca (Sea lettuce), Fucus ceranoides (Estuary Wrack) and Gracilaria gracilis (Slender Wart Weed), in kale (Brassica oleracea L.) seed germination and seedling development. The results showed that Gracilaria gracilis extract enhanced kale seed germination, presenting a higher seedlings weight (0.076 ± 0.004 g) and length (15.48 ± 0.59 cm), when compared with seedlings obtained in distilled water used as a control (weight = 0.059 ± 0.002 g; length = 13.10 ± 0.54 cm). Fucus ceranoides showed the lowest influence on seedling development (weight = 0.062 ± 0.002 g; length = 12.97 ± 0.59 cm). However, these results demonstrated that seaweed aqueous extracts can indeed enhance seed germination and seedling development.