bluuebunny/crossref_metadata_2025_split huggingface dataset:
An isolated crested newt population in Dutch coastal dunes: distribution relict or introduction?
Authors: de Brouwer Jurian , Helder Bas , France James , de Visser Manon C. , Struijk Richard P.J.H. , Wielstra Ben 
Published: December 2022

Abstract
Isolated distribution patches may represent local remnants of a formerly wider range or could have originated by human-mediated expansion beyond the natural range. Distinguishing between these two scenarios is not always straightforward. Northern crested newts (Triturus cristatus) in the Dutch coastal dunes are disconnected from the main species range by over 40 kilometres and whether they have been present historically is unclear. We genotyped crested newts from throughout the Netherlands for an mtDNA marker to determine the provenance of the coastal dune population. Because a closely related species, the Italian crested newt (T. carnifex), has an introduction history in the Netherlands, we also screened eight nuclear DNA SNP markers diagnostic for T. cristatus vs. T. carnifex. The crested newts from the coastal dunes carry a single T. cristatus mtDNA haplotype that naturally occurs in the south, but not the east, of the Netherlands. Therefore, we cannot distinguish if the population represents a natural distribution relict or is derived from an introduction. We find no evidence of genetic admixture with T. carnifex in the coastal dunes, but such admixture is apparent at another Dutch locality (far removed from a previously known genetically admixed population). Our study illustrates how difficult it can be to determine the origin of isolated populations.
Path Modification and Adjustments in Individual Behaviour? Evidence from Female Employment after the German Reunification
Authors: Fechter Charlotte 
Published: December 2022

Abstract
This paper is concerned with the question, how individuals behave during periods of institutional re-designs in welfare states. To understand behaviour after an institutional path modification, this article collects evidence on employment characteristics after the German reunification. East German women show to experience care activities for the first time later compared to West German women. Younger cohorts stay in care activities for a shorter period of time. Developments imply, that preferences have changed for women from both regions, proposing new forms of female employment standardisation in today’s Germany. At the same time, divergences from male employment characteristics are evident, implying rising gender inequality. Individual behaviour seems to be adaptive to new social processes proposed by institutions despite of former cultural beliefs.
Global Lagrangian Tracking of Continental Precipitation Recycling, Footprints, and Cascades
Authors: Cheng Tat Fan , Lu Mengqian 
Published: December 2022

Abstract
This study addresses how moisture in continental precipitation is recycled globally from a moisture-tracking perspective. Using a state-of-the-art three-dimensional Lagrangian model and optimized water accounting diagnostics, we complete a 40-yr (1971–2010) tracking of moisture from continental precipitation in the present-day climate. Climatologically, we conclude that 62% of continental precipitation stems from evapotranspiration through Lagrangian tracking—a measure is known as the global “continental precipitation recycling (CPR)” ratio. The result bridges the long-standing gap between the explicit (i.e., moisture trajectory–based) and implicit (i.e., water budget–based) estimates of the global CPR ratio in the literature. On the 1° grid scale, nonlocal terrestrial sources dominate precipitation in almost 70% of the land areas, most prominent in the continental interior. The CPR ratio consistently exhibits a contrasting seasonality between the mid-to-high latitudes and monsoon regions worldwide, from which two kinds of moisture source–regulated hydroclimate are generalized. Given the importance of CPR, we identify terrestrial source hotspots for continental precipitation that deserve careful governance. Using the backward “WaterSip” and a newly proposed forward “WaterDrip” diagnostics, we conceptualize and quantify two important types of cascading moisture recycling (CMR), whereby the moisture from an earlier source precipitates over an intermediate source and then re-evaporates to sustain precipitation farther downwind. The watershed-scale CMR metrics uncover the interdependence among the regional water cycles. Findings here advance the scientific understanding of the atmospheric water cycle as seen through Lagrangian tracking, and offer insights into transboundary watershed management and land-use planning to improve freshwater sustainability.

Significance Statement
Water is crucial for human civilization. There has been a century-long discussion on the moisture sources of continental precipitation. Using Lagrangian tracking, we show that 62% of continental precipitation stems from evapotranspiration, closing the gap with the budget-based estimate. Terrestrial sources dominate precipitation in 70% of Earth’s surface, especially in the interior of South America, Africa, and Eurasia. Global monsoon regions and the mid-to-high latitudes share a completely different source-regulated hydroclimate. Terrestrial source hotspots for continental precipitation that deserve conservation are identified. Two types of processes that formulate a cascade of regional water cycles are proposed and evaluated. Findings here advance the understanding of the origin of continental precipitation, offering insights into water and land management for freshwater sustainability.
Enhanced ENSO-Unrelated Summer Variability in the Indo–Western Pacific under Global Warming
Authors: Wang Chuan-Yang , Zheng Xiao-Tong , Xie Shang-Ping 
Published: December 2022

Abstract
El Niño–Southern Oscillation (ENSO) is an important but not the only source of interannual variability over the Indo–western Pacific. Non-ENSO forced variability in the region has received recent attention because of the implications for rainy-season prediction. Using a 35-member CESM1 Large Ensemble (CESM-LE) and 30 CMIP6 models, this study shows that the ensemble means project intensified interannual variability for precipitation, low-level winds, and sea level pressure under global warming, associated with the enhanced large-scale anomalous anticyclone (AAC) over the tropical northwestern (NW) Pacific after the ENSO signal is removed. A decomposition based on the column water vapor budget reveals that enhanced precipitation variability is due to the increased background specific humidity. The resultant anomalous diabatic heating intensifies the AAC, which further strengthens the precipitation anomalies. Over the tropical NW Pacific, the wind-induced evaporative cooling on the southeastern flank of the AAC is countered by the increased shortwave radiation due to the strengthened precipitation reduction. Tropospheric temperature anomalies in the ensemble means show no significant change, suggesting no apparent change of the interbasin positive feedback between the AAC and northern Indian Ocean SST. Intermodel analysis based on CMIP6 reveals that models with a larger increase in ENSO-unrelated precipitation variability over the NW Pacific are associated with stronger background warming in the eastern equatorial Pacific, due to the modulated Walker and Hadley circulations.
Effect of high temperature on mechanical properties and porosity of carbon fiber/epoxy composites
Authors: Wen Junjie , Wu Yi , Hou Xiao , Yan Mi , Xiao Yachao 
Published: December 2022

In this study, the mechanical behavior and porosity of a T700/epoxy composite material under high temperature conditions were investigated. The 0°/90° compression, tensile, interlaminar, and in-plane shear experiments of composite samples were performed at temperatures from 23°C to 170°C. The true strain–stress curves of composite samples were obtained. The cross section and fracture regions of the tested samples were analyzed by Scanning Electron Microscopy and X-ray tomography to understand the microstructure property relationships. It was observed that the mechanical performance of carbon fiber/epoxy composites generally decreased with increasing temperature. By comparing the fracture morphology, it was found that the fracture mechanism of the composites changed from interfacial debonding to matrix failure with increasing temperature. Three-dimensional visualization of porosities after mechanical experiments under high temperature conditions was reconstituted from X-ray tomography scan images. The results showed that the porosity fraction of the tested samples under high temperature conditions was generally higher than that under lower temperature conditions. Moreover, for the 90° tensile test, the diameter of the pores was mainly located at 4–12 μm and was almost constant along different distances to the failure edge. Meanwhile, compared with the tensile test, larger size of pores in samples of 90° compression tests were observed.
Expanding the Spectrum of Perioral Myogenic Tumors in Pediatric Patients: An SRF::NCOA2 Fused Perivascular Tumor of the Philtrum
Authors: Slack Jonathan C. , Hollowell Monica L. , Khouri Kimberly S. , Church Alanna J. , Ganske Ingrid M. , Delano Sophia , Al-Ibraheemi Alyaa 
Published: December 2022

Background: Perivascular tumors, which include myopericytoma and myofibroma, are rare benign soft tissue neoplasms composed of perivascular smooth muscle cells. Most demonstrate characteristic morphology and are readily diagnosed. However, a recently identified hypercellular subset shows atypical histologic features and harbor unique SRF gene fusions. These cellular perivascular tumors can mimic other more common sarcomas with myogenic differentiation. Methods: Clinical, radiological, morphological, immunohistochemical, and molecular findings were reviewed. Results: A slow-growing, fluctuant mass was noted within the philtrum at 16 months. Ultrasonography revealed a well-circumscribed cystic hypoechoic lesion. A small (1.0 cm), tan, well-circumscribed soft-tissue mass was excised after continued growth. Histologically, the encapsulated tumor was hypercellular and composed of spindle cells with predominantly-storiform architecture, focal perivascular condensation, dilated branching thin-walled vessels, increased mitoses, and a smooth muscle immunophenotype. An SRF::NCOA2 fusion was identified. Conclusion: We report the first case of an SRF-rearranged cellular myopericytoma in the perioral region in a young child. This case expands the differential diagnosis of perioral soft tissue tumors with myogenic differentiation. We highlight key clinical, pathological, and molecular features. As we illustrate, these rare tumors pose a considerable diagnostic challenge, and risk misdiagnosis as sarcoma, most notably spindle cell rhabdomyosarcoma.
Community pharmacists’ perceptions of the CARD (Comfort Ask Relax Distract) system for use during vaccinations
Authors: Gudzak Victoria , Freedman Tamlyn , Logeman Charlotte , Bucci Lucie M. , Taddio Anna 
Published: December 2022

Introduction: We explored Canadian community pharmacists’ perceptions of the CARD (Comfort Ask Relax Distract) system, a vaccine delivery framework that integrates evidence-based interventions that reduce immunization stress-related responses (ISRRs). The objective was to introduce CARD to pharmacists and obtain their feedback to guide future implementation efforts for community pharmacy-based vaccinations. Methods: Eighteen pharmacists across all provinces participated in one-on-one moderated virtual interviews. A semistructured interview guide was used to guide the discussion and included questions about experiences with coronavirus disease 2019 (COVID-19) vaccine administration and perceptions of CARD as a framework for vaccination delivery in the pharmacy. In this report, we focus on feedback about CARD. Interviews were audio-recorded and transcribed verbatim and deductively coded using the Consolidated Framework for Implementation Research (CFIR). Results: The study was conducted from May 28 to August 16, 2021. Pharmacists’ responses were categorized in 3 CFIR domains: intervention characteristics, inner setting and characteristics of individuals. Overall, pharmacists were receptive towards CARD and thought it filled a current gap in practice, particularly for childhood vaccinations. Pharmacists reported learning techniques to make vaccinations more comfortable for their clients. They provided feedback on how specific interventions could be adapted for their practice (e.g., dissemination of client-directed education via appointment booking portals, provision of distraction items in waiting and vaccinating spaces). Discussion: This study provided preliminary evidence that pharmacists found CARD acceptable and appropriate for community pharmacy-based vaccinations. The data collected from this study were used to inform a small-scale implementation project with CARD during COVID-19 vaccine administration in children aged 5 to 11 years.
Anti-FVIII antibodies in Black and White hemophilia A subjects: do F8 haplotypes play a role?
Authors: Pratt Kathleen P. , Gunasekera Devi , Vir Pooja , Tan Siyuan , Pierce Glenn F. , Olsen Cara , Butenas Saulius , Mann Kenneth G. 
Published: December 2022

Abstract
The most common complication in hemophilia A (HA) treatment, affecting 25% to 30% of patients with severe HA, is the development of alloimmune inhibitors that foreclose the ability of infused factor VIII (FVIII) to participate in coagulation. Inhibitors confer significant pathology on affected individuals and present major complexities in their management. Inhibitors are more common in African American patients, and it has been hypothesized that this is a consequence of haplotype (H)-treatment product mismatch. F8 haplotypes H1 to H5 are defined by nonsynonymous single-nucleotide polymorphisms encoding sequence variations at FVIII residues 1241, 2238, and 484. Haplotypes H2 to H5 are more prevalent in individuals with Black African ancestry, whereas 80% to 90% of the White population has the H1 haplotype. This study used an established multiplex fluorescence immunoassay to determine anti-FVIII antibody titers in plasma from 394 individuals with HA (188 Black, 206 White), measuring their binding to recombinant full-length H1 and H2 and B-domain–deleted (BDD) H1/H2, H3/H5, and H4 FVIII proteins. Inhibitor titers were determined using a chromogenic assay and linear B-cell epitopes characterized using peptide microarrays. FVIII-reactive antibodies were readily detected in most individuals with HA, with higher titers in those with a current inhibitor, as expected. Neither total nor inhibitory antibody titers correlated with F8 haplotype mismatches, and peptides with D1241E and M2238V polymorphisms did not comprise linear B-cell epitopes. Interestingly, compared with the full-length FVIII products, the BDD-FVIII proteins were markedly more reactive with plasma antibodies. The stronger immunoreactivity of BDD-FVIII suggests that B-domain removal might expose novel B-cell epitopes, perhaps through conformational rearrangements of FVIII domains.
JAK/STAT blockade reverses the malignant phenotype of Hodgkin and Reed-Sternberg cells
Authors: Fernández Sara , Solórzano Jose L. , Díaz Eva , Menéndez Victoria , Maestre Lorena , Palacios Sara , López Mar , Colmenero Argentina , Estévez Mónica , Montalbán Carlos , Martínez Ángel , Roncador Giovanna , García Juan F. 
Published: December 2022

Abstract
Constitutive activation of the JAK/STAT pathway is a common phenomenon in classic Hodgkin lymphoma (cHL). The clinical potential of anti-JAK/STAT therapy is being explored in early-stage clinical trials. Notwithstanding, very little information is available about the complex biological consequences of this blockade. Here, we investigated the effects of JAK/STAT pharmacological inhibition on cHL cell models using ruxolitinib, a JAK 1/2 inhibitor that induces apoptosis by concentration- and time-dependent mechanisms. An unbiased whole-transcriptome approach identified expression of the anti-GCSF receptor (CSF3R) as a potential surrogate biomarker of JAK/STAT overactivation. In addition, longitudinal gene expression analyses provided further mechanistic information about pertinent biological pathways involved, including 37 gene pathways distributed in 3 main clusters: cluster 1 was characterized by upregulation of the G2/M checkpoint and major histocompatibility complex-related clusters; 2 additional clusters (2 and 3) showed a progressive downregulation of the tumor-promoting inflammation signatures: JAK/STAT and interleukin 1 (IL-1)/IL-4/IL-13/IL-17. Together, our results confirm the therapeutic potential of JAK/STAT inhibitors in cHL, identify CSF3R as a new biomarker, and provide supporting genetic data and mechanistic understanding.
Impact of sewage water irrigation on Datura innoxia grown in sandy loam soil
Authors: Abeed Amany H. A. , Ali Mohammed , Eissa Mamdouh A. , Tammam Suzan A. 
Published: December 2022

AbstractBackgroundA potential solution for recycling and reusing the massively produced sewage water (SW) is to irrigate certain plants instead of highly cost recycling treatment. Although the extensive and irrational application of SW may cause environmental pollution thus, continual monitoring of the redox status of the receiver plant and the feedback on its growth under application becomes an emergent instance. The impact of SW, along with well water (WW) irrigation of medicinal plant,Datura innoxia, was monitored by some physio-biochemical indices.ResultsThe SW application amplified the growth, yield, minerals uptake, and quality ofD. innoxiaplants compared to the WW irrigated plants. The total chlorophyll, carotenoid, non-enzymatic antioxidants, viz. anthocyanin, flavonoids, phenolic compounds, and total alkaloids increased by 85, 38, 81, 50, 19, and 37%, respectively, above WW irrigated plants. The experiment terminated in enhanced leaf content of N, P, and K by 43, 118, and 48%, respectively. Moreover, stimulation of carbon and nitrogen metabolites in terms of proteins, soluble sugars, nitrate reductase (NR) activity, and nitric oxide (NO) content showed significant earliness in flowering time. The SW application improved not onlyDaturaplants’ quality but also soil quality. After four weeks of irrigation, the WW irrigated plants encountered nutrient deficiency-induced stress evidenced by the high level of proline, H2O2, and MDA as well as high enzyme capabilities. Application of SW for irrigation ofD. innoxiaplant showed the improvement of secondary metabolites regulating enzyme phenylalanine ammonia-lyase (PAL), restored proline content, and cell redox status reflecting high optimal condition for efficient cellular metabolism and performance along the experiment duration.ConclusionsThese evidences approved the benefits of practicing SW to improve the yield and quality ofD. innoxiaand the feasibility of generalization on multipurpose plants grown in poor soil.
The value of the ACEF II score in Chinese patients with elective and non-elective cardiac surgery
Authors: Mo Zhiming , Hu Penghua , Xie Zhiyong , Wu Yanhua , Li Zhilian , Fu Lei , Chen Yuanhan , Liang Xinling , Liang Huaban , Dong Wei 
Published: December 2022

Abstract
Objective
To evaluate the value of the ACEF II score in predicting postoperative hospital death and acute kidney injury requiring dialysis (AKI-D) in Chinese patients.

Methods
This retrospective study included adult patients who underwent cardiopulmonary bypass open heart surgery between January 2010 and December 2015 at Guangdong Provincial People’s Hospital. ACEF II was evaluated to predict in-hospital death and AKI-D using the Hosmer–Lemeshow goodness of fit test for calibration and area under the receiver operating characteristic (ROC) curve for discrimination in non-elective and elective cardiac surgery.

Results
A total of 9748 patients were included. Among them, 1080 underwent non-elective surgery, and 8615 underwent elective surgery. Mortality was 1.8% (177/9748). In elective surgery, the area under the ROC (AUC) of the ACEF II score was 0.704 (95% CI: 0.648–0.759), similar to the ACEF score of 0.709 (95% CI: 0.654–0.763). In non-elective surgery, the AUC of the ACEF II score was 0.725 (95% CI: 0.663–0.787), higher than the ACEF score (AUC = 0.625, 95% CI: 0.553–0.697). The incidence of AKI-D was 3.5% (345/9748). The AUC of the ACEF II score was 0.718 (95% CI: 0.687–0.749), higher than the ACEF score (AUC = 0.626, 95% CI: 0.594–0.658).

Conclusion
ACEF and ACEF II have poor discrimination ability in predicting AKI-D in non-elective surgery. The ACEF II and ACEF scores have the same ability to predict in-hospital death in elective cardiac surgery, and the ACEF II score is better in non-elective surgery. The ACEF II score can be used to assess the risk of AKI-D in elective surgery in Chinese adults.
Circ_0004104 participates in the regulation of ox-LDL-induced endothelial cells injury via miR-942-5p/ROCK2 axis
Authors: Zhang Yuanyuan , Wang Shaojun , Guo Sicong , Zhang Xinzhong , Yang Chuan , Su Guangsheng , Wan Jiye 
Published: December 2022

Abstract
Background
Cardiovascular disease was the most common disease among the elderly with high morbidity and mortality. Circ_0004104 was demonstrated to be involved in the regulation of atherosclerosis.

Methods
Quantitative real-time polymerase chain reaction was employed to measure the expression of circ_0004104, miR-942-5p and Rho associated coiled-coil containing protein kinase 2 (ROCK2). Cell proliferation was tested by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Cell apoptosis was measured by flow cytometry, and tube formation assay was used to detect the angiogenesis ability of cells. Western blot assay was performed to assess protein levels. Enzyme‑linked immunosorbent assay was used to detect the release of IL-1β and TNF-α. The relationship between miR-942-5p and circ_0004104 or ROCK2 was identified by dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, and RNA pull-down assay.

Results
Oxidized low-density lipoprotein (ox-LDL) inhibited the proliferation of human umbilical vein endothelial cells (HUVECs) and promoted apoptosis in a dose-dependent manner. Circ_0004104 was increased in serum of atherosclerosis patients and ox-LDL-treated HUVECs, and silence of circ_0004104 promoted the proliferation of ox-LDL-exposed HUVECs and inhibited cell apoptosis. MiR-942-5p downregulation reversed si-circ_0004104-mediated influences in HUVECs upon ox-LDL exposure. ROCK2 was the target of miR-942-5p and circ_0004104 regulated the expression of ROCK2 through sponging miR-942-5p. ROCK2 abated the influences of miR-942-5p in ox-LDL-stimulated HUVECs. Circ_0004104 was increased in the exosomes derived from ox-LDL-exposed HUVECs, and the expression of circ_0004104 was promoted in HUVECs after stimulation with ox-LDL-treated HUVECs cells-derived exosomes.

Conclusion
Circ_0004104 downregulation receded ox-LDL-induced injury in HUVECs through miR-942-5p and ROCK2.