bluuebunny/crossref_metadata_2025_split huggingface dataset:
A 10-year retrospective analysis ofToxoplasma gondiiqPCR screening in allogeneic hematopoietic stem cell transplantation recipients
Authors: Xhaard Alienor , Villate Alban , Hamane Samia , Michonneau David , Menotti Jean , Robin Marie , Sicre de Fontbrune Flore , Dhédin Nathalie , Peffault de la Tour Régis , Socié Gérard , Bretagne Stéphane 
Published: June 2022

AbstractWeekly bloodToxoplasma gondiiDNA screening using real-time quantitative polymerase chain reaction (qPCR) has been implemented in all allogeneic hematopoietic stem cell transplantation (alloHSCT) recipients at our hospital. We retrospectively analyzed the consequences of a positive blood qPCR in the management ofToxoplasmainfection (TI) and disease (TD).From 2011 to 2020, 52 (4.13%) of 1 257 alloHSCT recipients had at least one positive qPCR, 45 (3.5%) with TI and seven (0.56%) with TD (central nervous system involvement). Forty-four patients were qPCR-positive before day 100, 30 without and 14 with anti-Toxoplasmaprophylaxis. Twenty-five of them (56.8%) started or continued prophylactic dosage treatment: all became qPCR-negative, including 20 (80%) receiving only prophylactic dosage treatment. Twenty-four of them (54.5%) received non-prophylactic dosage treatment: qPCR became negative in 22/24 (91.7%), while TI contributed to death in two cases. Six of the eight patients diagnosed after D100 were breakthrough TI or TD. No death was attributable to TI or TD.qPCRkinetics available for 24 patients increased until anti-Toxoplasmatreatment began, then decreased with all treatment regimens.Clinical follow-up and qPCR monitoring with quantification of the parasitic load appears a reasonable strategy to avoid TD and to use minimal effective dosage of anti-Toxoplasmatreatments.
Inflammasome activation and pulmonary viral loads define two distinct clinical outcomes in COVID-19
Authors: de Sá Keyla S.G. , Amaral Luana A. , Caetano Camila C.S. , Becerra Amanda , Batah Sabrina S. , de Oliveira Isadora M. , Lopes Letícia S. , Almeida Leticia , Oliveira Samuel , Wada Danilo Tadao , Koenigkam-Santos Marcel , Martins Ronaldo B. , Rosales Roberta R. C. , Arruda Eurico , Fabro Alexandre T , Zamboni Dario S. 
Published: June 2022

AbstractCOVID-19 has affected more than half a billion people worldwide, with more than 6.3 million deaths, but the pathophysiological mechanisms involved in lethal cases and the host determinants that determine the different clinical outcomes are still unclear. In this study, we assessed lung autopsies of 47 COVID-19 patients and examined the inflammatory profiles, viral loads, and inflammasome activation. Additionally, we correlated these factors with the patient’s clinical and histopathological conditions. Robust inflammasome activation, mediated by macrophages and endothelial cells, was detected in the lungs of lethal cases of SARS-CoV-2. An analysis of gene expression allowed for the classification of COVID-19 patients into two different clusters. Cluster 1 died with higher viral loads and exhibited a reduced inflammatory profile than Cluster 2. Illness time, mechanical ventilation time, pulmonary fibrosis, respiratory functions, histopathological status, thrombosis, viral loads and inflammasome activation significantly differed between the two clusters. Our data demonstrated two distinct profiles in lethal cases of COVID-19, thus indicating that the balance of viral replication and inflammasome-mediated pulmonary inflammation led to different clinical outcomes. We provide important information to understand clinical variations in severe COVID-19, a process that is critical for decisions between immune-mediated or antiviral-mediated therapies for the treatment of critical cases of COVID-19.
The most common human ADAR1p150 Zα domain mutation P193A is well tolerated in mice and does not activate the integrated stress response pathway
Authors: Liang Zhen , Taylor Scott , Goradia Ankita , Heraud-Farlow Jacki , Walkley Carl R 
Published: June 2022

SummaryADAR1 mediated A-to-I RNA editing is a self/non-self discrimination mechanism for cellular double stranded RNAs. ADAR mutations are one cause of Aicardi-Goutières Syndrome, an inherited paediatric encephalopathy, broadly classed as a “Type I interferonopathy”. The most common ADAR1 mutation is a proline 193 alanine (p.P193A) mutation, mapping to the ADAR1p150 isoform specific Zα domain. We report the development of an independent murine P195A knock-in mouse, homologous to the human P193A mutation. The Adar1P195A/P195A mice are largely normal and the mutation is well tolerated. Contrasting with previous reports when the P195A mutation was compounded with an ADAR1 null allele, the majority of mice have only a modest reduction in weaning weight and survived long-term. Severe runting and shortened survival of Adar1P195A/-animals are dependent on the parental genotype. The P195A mutation is well tolerated in vivo and the loss of MDA5 is sufficient to completely rescue the Adar1P195A/- mice.
Codistribution as an indicator of whole metacommunity response to environmental change
Authors: Terry J. Christopher D. , Langdon William , Rossberg Axel G. 
Published: June 2022

AbstractMetacommunity structure can be summarised by fitting joint species distribution models and partitioning the variance explained into environmental, spatial and codistribution components. Here we identify how these components respond through time with directed environmental change and propose this as an indicator of sustained directional pressure. Through simulations, we identify how declines in the codistribution component can diagnose ecological breakdown, while rises in environmental and spatial components may indicate losses in peripheral areas and dispersal limitations. We test the method in two well-studied systems. Butterflies are known to be strongly responding to climate change, and we show that over 21 years the codistribution component declines for butterfly communities in southern England. By contrast, birds in the same region are under less climate pressure and, despite high occupancy turnover, show minimal change in metacommunity structure. The approach has high potential to summarise and compare the impact of external drivers on whole communities.
Single-shot Volumetric Chemical Imaging by Mid-Infrared Photothermal Fourier Light Field Microscopy
Authors: Zhang Yi , Jia Danchen , Yang Qianwan , Xue Yujia , Tan Yuying , Guo Zhongyue , Zhang Meng , Tian Lei , Cheng Ji-Xin 
Published: June 2022

AbstractThree-dimensional molecular imaging of living organisms and cells plays a significant role in modern biology. Yet, current volumetric imaging modalities are largely fluorescence-based and thus lack chemical content information. Mid-infrared photothermal microscopy as a new chemical imaging technology provides infrared spectroscopic information at sub-micrometer spatial resolution. Here, by harnessing thermosensitive fluorescent dyes to sense the mid-infrared photothermal effect, we demonstrate mid-infrared photothermal Fourier light field (MIP-FLF) microscopy for single-shot volumetric infrared spectroscopic imaging at the speed of 8 volumes per second and sub-micron spatial resolution. Protein contents in bacteria and lipid droplets in living pancreatic cancer cells are visualized. Altered lipid metabolism in drug-resistant pancreatic cancer cells is observed with the MIP-FLF microscope.
Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
Authors: Fan Lusheng , Gao Bin , Xu Ye , Flynn Nora , Le Brandon , You Chenjiang , Li Shaofang , Achkar Natalia , Manavella Pablo A. , Yang Zhenbiao , Chen Xuemei 
Published: June 2022

AbstractMicroRNAs (miRNAs) play an essential role in plant growth and development, and as such, their biogenesis is fine-tuned via regulation of the core microprocessor components. Here, we report thatArabidopsisAAR2, a homolog of a U5 snRNP assembly factor in yeast and humans, not only acts in splicing but also promotes miRNA biogenesis. AAR2 interacts with the microprocessor component HYPONASTIC LEAVES1 (HYL1) in the cytoplasm, nucleus and dicing bodies. Inaar2mutants, abundance of nonphosphorylated HYL1, the active form of HYL1, and the number of HYL1-labeled dicing bodies are reduced. Primary miRNA (pri-miRNA) accumulation is compromised despite normal promoter activities ofMIRgenes inaar2mutants. RNA decay assays show that theaar2-1mutation leads to faster degradation of pri-miRNAs in a HYL1-dependent manner, which reveals a previously unknown and negative role of HYL1 in miRNA biogenesis. Taken together, our findings reveal a dual role of AAR2 in miRNA biogenesis and pre-mRNA splicing.SignificanceIn yeast and humans, AAR2 is involved in pre-mRNA splicing through regulating U5 snRNP assembly. This study shows thatArabidopsisAAR2 promotes microRNA (miRNA) accumulation in addition to its conserved role in pre-mRNA splicing. AAR2 is associated with the microprocessor component HYL1 and promotes its dephosphorylation to produce the active form in miRNA biogenesis. The study also reveals a previously unknown role of HYL1 in causing the degradation of the primary precursors to miRNAs (pri-miRNAs) and a role of AAR2 in protecting pri-miRNAs from HYL1-depedent degradation. Taken together, our findings provide new insights into the role of a conserved splicing factor in miRNA biogenesis in plants.
Antiviral T-cell Biofactory platform for SARS-CoV-2
Authors: Ssemadaali Marvin A. , Newmyer Sherri , Radhakrishnan Harikrishnan , Arredondo Juan , Javitz Harold S. , Dandekar Satya , Bhatnagar Parijat 
Published: June 2022

1.0AbstractVaccines help reduce new infections, but interventions that can prevent the disease from transitioning to a severe stage are rather limited. Dysregulated IFN kinetics are mostly exploited by pathogenic viruses, including SARS-CoV-2. The clinical benefits of systemically infused IFN are, unfortunately, mired by undesired side effects. To address this situation, we engineered a T cell to synthesize interferons (IFNs) as antiviral proteins upon recognizing the virus envelop protein of SARS-CoV-2, i.e.,anti-SARS T-cell Biofactory. The T-cell Biofactory, capable of regulating the IFN expression with spatiotemporal resolution within the infected tissues, can mitigate these concerns. In this work, we determined the prophylactic and therapeutic effects of the type-I and type-III IFNs produced from the T-cell Biofactory against SARS-CoV-2 infection in host cells and investigated the expression profiles of ensuing IFN-stimulated genes (ISGs). To enable the translation of T-cell Biofactory as an effective antiviral countermeasure, we also investigated an irradiation dose that renders the T-cell Biofactory non-proliferative and thus non-oncogenic. The ongoing public health crisis motivated us to direct the T-cell Biofactory technology to target SARS-CoV-2. The T-cell Biofactory, based on T cells engineered with chimeric antigen receptors (CAR T cells), is a platform technology that can be rapidly re-engineered and become available for targeting any new pathogen.
Effect of educational intervention based on theory of planned behavior on aggression preventive behaviors in students
Authors: Khaleghi Ali Asghar , Jafarzadeh Saeedeh , Afzali Harsini Pooyan , Kashfi Seyyed Hannan , Mohammadkhah Fatemeh , Khani Jeihooni Ali 
Published: June 2022

Purpose
Aggressive behaviors are common among students. Given the importance of education in reducing aggressive behavior, this study aims to examine the influence of a theory of planned behavior (TPB)-based educational intervention on aggression-preventive behaviors in fifth-grade male students in Fasa city, Iran.

Design/methodology/approach
A total of 120 male students were randomly picked from several public schools in Fasa city, Iran, between 2018 and 2019. Assessments were completed before and three months after the intervention. Intervention consisted of eight 55–60-min training sessions based on the TPB’s processes and constructs.

Findings
Prior to the educational intervention, there was no significant difference between the two groups in terms of attitude, subjective norms, perceived behavioral control, intention to stop aggressiveness or to engage in aggression-preventive activities. However, three months following the intervention, the experimental group demonstrated a considerable rise in the aforementioned constructs (p > 0.001 in all cases).

Originality/value
The study’s findings demonstrated the efficacy of a TPB-based educational intervention on constructs of the TPB theory. Results suggest application of a TPB-based educational intervention can have a pro-social impact on the attitudes and behavior of elementary age boys.
Carbon emission reduction and green marketing decisions in a two-echelon low-carbon supply chain considering fairness concern
Authors: Sun Hongxia , Zhong Yang 
Published: June 2022

Purpose
The purpose of this paper is to investigate the impact of fairness concern on the optimal pricing, carbon emission reduction (CER), green marketing efforts (GME) and utility of supply chain members in a two-echelon low-carbon supply chain composed of one manufacturer and one retailer. First, three basic models that consider the manufacturer’s different attitudes toward the retailer’s fairness concern are constructed. The optimal decisions of these models are obtained. Second, these optimal solutions are compared, and the effects of some key parameters including fairness concern on the optimal decisions and utility are examined for the three models. Furthermore, the manufacturer may misestimate the retailer’s fairness concern; therefore, an extended model is proposed.

Design/methodology/approach
The authors adopt the manufacturer-led Stackelberg game theoretic framework, where the manufacturer decides the wholesale price and CER level and, then, the retailer determines the retail price and GME.

Findings
The results show that fairness concern has a negative impact on the wholesale price, the level of CER and GME, and fairness concern are not always beneficial for maximizing utility, although it is related to whether the manufacturer pays attention to the retailer’s fairness concern. The manufacturer will gain more utility when considering the fairness concern of retailers than non-consideration. Overestimating or underestimating the fairness concern of the retailers does not lead to benefits for the manufacturer.

Research limitations/implications
This study has the following two limitations that need to be addressed in future research. First, the authors only consider the fairness concern of a single retailer but not peer-induced fairness among multiple competing retailers, which can be taken into account in future studies. Second, the demand function is linearly related to price, CER and GME. Because of the uncertainty of market information, the uncertainty demand function can be further considered.

Originality/value
This paper simultaneously considers the factors CER, GME and fairness concern. The utility function of the retailer is established according to taking the Nash bargaining solution as a fairness reference point, and four different models are constructed and compared.
Efficiency of temporal sensor data compression methods to reduce LoRa-based sensor node energy consumption
Authors: Väänänen Olli , Hämäläinen Timo 
Published: June 2022

Purpose
Minimizing the energy consumption in a wireless sensor node is important for lengthening the lifetime of a battery. Radio transmission is the most energy-consuming task in a wireless sensor node, and by compressing the sensor data in the online mode, it is possible to reduce the number of transmission periods. This study aims to demonstrate that temporal compression methods present an effective method for lengthening the lifetime of a battery-powered wireless sensor node.

Design/methodology/approach
In this study, the energy consumption of LoRa-based sensor node was evaluated and measured. The experiments were conducted with different LoRaWAN data rate parameters, with and without compression algorithms implemented to compress sensor data in the online mode. The effect of temporal compression algorithms on the overall energy consumption was measured.

Findings
Energy consumption was measured with different LoRaWAN spreading factors. The LoRaWAN transmission energy consumption significantly depends on the spreading factor used. The other significant factors affecting the LoRa-based sensor node energy consumption are the measurement interval and sleep mode current consumption. The results show that temporal compression algorithms are an effective method for reducing the energy consumption of a LoRa sensor node by reducing the number of LoRa transmission periods.

Originality/value
This paper presents with a practical case that it is possible to reduce the overall energy consumption of a wireless sensor node by compressing sensor data in online mode with simple temporal compression algorithms.
Carbon Isotope Stratigraphy of the Cambrian System in the Eastern Tarim Basin, China
Authors: WANG Huajian , SU Jin , WANG Xiaomei , YE Yuntao , WANG Yu , FANG Yu , ZHANG Shuichang 
Published: June 2022

AbstractThe ultra‐deep Cambrian System in the Tarim Basin is an important field for petroleum exploration, while fine division of the Cambrian strata remains controversial. In recent years, carbon isotope stratigraphy of the Cambrian System has been established and widely used. Here, we report an integrated profile of carbonate and organic carbon isotopic values (δ13Ccarb and δ13Corg) from cuttings of the Tadong2 Well in the eastern Tarim Basin. Three carbon isotope anomalies of BACE, ROECE and SPICE were recognized on the δ13Ccarb profile. Three apogees and a nadir on the δ13Corg profile and the onset of ROECE on the δ13Ccarb profile were suggested as boundaries of the present four series of the Cambrian System. Suggested boundaries are easily identifiable on the gamma logging profile and is consistent with the previous division scheme, based on biostratigraphic evidence in outcrop sections. Abnormal carbon cycle perturbations and organic carbon burials during the BACE and SPICE events might be related to the reduction and expansion of a huge dissolved organic carbon reservoir in the deep ocean of the ancient Tarim Basin.
Prevalence of early childhood obesity in Ireland: Differences over time, between sexes and across child growth criteria
Authors: Jabakhanji Samira Barbara , Boland Fiona , Ward Mark , Biesma Regien 
Published: June 2022

SummaryBackgroundVarious child growth criteria exist for monitoring overweight and obesity prevalence in young children.ObjectivesTo estimate early overweight and obesity prevalence in Ireland and compare the differences in prevalence across ages, growth criteria and sexes.MethodsLongitudinal body mass index data from the nationally representative Growing Up in Ireland infant cohort (n = 11 134) were categorized (‘under‐/normal weight’, ‘risk of overweight’, ‘overweight’, ‘obesity’) using the sex‐ and age‐specific International Obesity Task Force growth reference, World Health Organization growth standard and World Health Organization growth reference criteria. Differences in prevalences between criteria and sexes, and changes in each weight category and criterion across ages (9 months, 3 years, 5 years), were investigated.ResultsAcross criteria, 11%–40% of children had overweight or obesity at 9 months, 14%–46% at 3 years and 8%–32% at 5 years of age. Prevalence estimates were highest using the World Health Organization growth reference, followed by International Obesity Task Force estimates. Within each criterion, prevalence decreased significantly over time (p < 0.05). However, when combining both World Health Organization criteria, as recommended for population studies, prevalence increased, due to differences in definitions between them. Significantly more boys than girls had overweight/obesity using either World Health Organization criterion, which was reversed using the International Obesity Task Force growth reference.ConclusionsTo increase transparency and comparability, studies of childhood obesity need to consider differences in prevalence estimates across growth criteria. Effective prevention, intervention and policy‐making are needed to control Ireland's high overweight and obesity prevalence.
What are the odds of burnt‐out risk and leaving the job? Turnover intent consequences of worker burnout using a two sample New Zealand study
Authors: Haar Jarrod 
Published: June 2022

AbstractJob burnout is a pressing issue for organizations, and this study explores the new Burnout Assessment Tool (BAT), which provides a robust calculation of burnt‐out risk. Next, the odds of high turnover intentions from burnt‐out risk are calculated using two samples: (1) N = 709 employees and (2) N = 313 managers. Analysis shows the odds of burnt‐out risk are higher for managers (17%) than employees (8%). High burnt‐out risk in employees shows a 47% likelihood of high turnover intent versus 13% for employees with nonburnt‐out risk. High burnt‐out risk in managers shows a 51% likelihood of high turnover intent versus 12% for managers with nonburnt‐out risk. Furthermore, moderating effects of supervisor organizational embodiment were found to interact with burnt‐out risk for employees only, showing the highest turnover intent when embodiment is high, reflecting the potential backlash against the organization.
Perspectivas históricas de la Revista Internacional del Trabajo 1921‐2021: un siglo de investigación sobre el mundo del trabajo1
Authors: HOEHTKER Dorothea 
Published: June 2022

ResumenEn este artículo se analiza la historia de la Revista Internacional del Trabajo (RIT), una importante publicación periódica de la Organización Internacional del Trabajo (OIT) creada en 1921 conforme a lo dispuesto en el artículo 396 del Tratado de Versalles de 1919. Se estudia, desde diversas perspectivas, la transformación del antiguo boletín institucional polivalente en la moderna revista académica que es hoy, atendiendo a su trayectoria institucional, la función de los editores y los perfiles profesionales y académicos de los autores. Se investiga la contribución de la RIT a los debates académicos y de política y su importancia para la OIT, examinando desde una perspectiva histórica los contenidos, temas y ámbitos geográficos de los casi 3 000 artículos publicados hasta la fecha.
Defining Basque music: Charles Bordes' (1863–1909) musical regionalism and influence in the Basque Country and Paris
Authors: Odriozola Otamendi Asier 
Published: June 2022

AbstractThis paper analyses the influence of the French composer and musicologist Charles Bordes (1863–1909) on the definition of ‘Basque music’ both in the peninsular Basque Country and in Paris. On the one hand, it studies Bordes' ideological background, his vision of traditional Basque music and the acceptance of his shared musicological legacy in Basque regionalist and nationalist circles. On the other hand, it pays particular attention to the reception of his ideas in the Parisian musical milieu through the analysis of the criticism aroused by the premieres of two Basque‐themed operas: Ramuntcho (1908) and Chiquito (1909). This article frames Bordes' activity within the regionalist movement in France. However, it also draws attention to the transnational and ambivalent nature of regionalism, especially in cross‐border spaces, where regionalist discourses could take divergent forms according to the different nationalist agendas with which they were associated.