bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plural forms and differentiation strategies in the agri‐food sector: Evidence from the U.S. wine industry
Authors: Miranda Bruno V. , Ross Brent , Franken Jason , Gómez Miguel 
Published: April 2022

AbstractThis article investigates how measurement ability, trust, and the pursuit of differentiation strategies influence the adoption of plural forms in an agri‐food system. We analyze a unique dataset of 314 winegrape sourcing choices from 74 wineries located in five U.S. states, investigating how the pursuit of differentiation influences the likelihood of adoption of a plural form—that is, the simultaneous adoption of a formal arrangement (i.e., vertical integration or long‐term formal contract) and an informal arrangement (i.e., informal contracts or spot‐market transactions). Consistent with previous studies, measurement ability and the existence of trust are both associated with a higher likelihood of adoption of informal arrangements in comparison to formal arrangements. Moreover, we show that the likelihood of adoption of a plural form increases as the differentiation potential of a winegrape variety also increases. Finally, we find that the use of plural forms is associated with a reduction in the importance of trust in the relationships with external suppliers. This study is important because it sheds light on the role of plural forms in the pursuit of competitive advantage in an agri‐food system. Overall, it suggests that the current interpretations of the idea of “vertical coordination” may be enlarged to encompass a greater deal of organizational diversity.
Sequential Nucleophilic Addition and Regioselective Ring‐Expansion Reactions of α‐Diazophosphonates with Isatins: Access to 4‐Phosphonylated‐3‐hydroxyquinolin‐2(1H)‐ones
Authors: Zhao Nannan , Bai Yan , Liu Yujia , Xiao Shan , Wang Jihui , Wang Lei 
Published: April 2022

AbstractA sequential protocol of α‐diazophosphonates with isatins to access a series of α‐diazo‐β‐hydroxyphosphonate derivatives via the inorganic base catalysis was reported. The resulting α‐diazo‐β‐hydroxyphosphonates could then be readily transformed to 4‐phosphonylated‐3‐hydroxyquinolin‐2(1H)‐ones with moderate to excellent yields through a catalyst‐free regioselective ring‐expansion rearrangement. Control experiment demonstrates that intramolecular cyclization pathway is more reasonable for the ring‐expansion process. In addition, a benzo[b]thiophene‐derived isatin featured with the inhibition of SARS‐CoV Mpro was also suitable for this transformation and generated the corresponding scaffolds with potential anti‐virus activities for further development.
Transfer of Axial Chirality to the Nanoscale Endows Carbon Nanodots with Circularly Polarized Luminescence
Authors: Di Noja Simone , Amato Francesco , Zinna Francesco , Di Bari Lorenzo , Ragazzon Giulio , Prato Maurizio 
Published: April 2022

AbstractWe report the synthesis, purification and characterization of chiral carbon nanodots starting from atropoisomeric precursors. The obtained atropoisomeric carbon nanodots are soluble in organic solvents and have good thermal stability, which are desirable features for technological applications. The synthetic protocol is robust, as it supports a number of variations in terms of molecular doping agents. Remarkably, the combination of axially chiral precursors and 1,4‐benzoquinone as doping agent results in green‐emissive carbon dots displaying circularly polarized luminescence. Dissymmetry factors of |3.5|×10−4 are obtained in solution, without the need of any additional element of chirality. Introducing axial chirality expands the strategies available to tailor the properties of carbon nanodots, paving the way for carbon nanoparticles that combine good processability in organic solvents with engineered advanced chiroptical properties.
Malarial PI4K inhibitor induced diaphragmatic hernias in rat: Potential link with mammalian kinase inhibition
Authors: Demarta‐Gatsi Claudia , Donini Cristina , Duffy James , Sadler Claire , Stewart Jane , Barber Jane A. , Tornesi Belen 
Published: April 2022

AbstractBackgroundMMV390048 is an aminopyridine plasmodial PI4K inhibitor, selected as a Plasmodium blood‐stage schizonticide for a next generation of malaria treatments to overcome resistance to current therapies. MMV390048 showed an acceptable preclinical safety profile and progressed up to Phase 2a clinical trials. However, embryofetal studies revealed adverse developmental toxicity signals, including diaphragmatic hernias and cardiovascular malformations in rats but not rabbits.MethodsIn vivo exposures of free plasma concentrations of compound in rats were assessed in relation to in vitro human kinase inhibition by MMV390048, using the ADP‐Glo™ Kinase Assay.ResultsWe demonstrate a potential link between the malformations seen in the embryofetal developmental (EFD) studies and inhibition of the mammalian PI4Kβ paralogue, as well as inhibition of the off‐target kinases MAP4K4 and MINK1. PI3Kγ may also play a role in the embryofetal toxicity as its in vitro inhibition is covered by in vivo exposure. The exposures in the rabbit embryofetal development studies did not reach concentrations likely to cause PI4K inhibition. Overall, we hypothesize that the in vivo malformations observed could be due to inhibition of the PI4K target in combination with the off‐targets, MAP4K4 and MINK1. However, these relationships are by association and not mechanistically proven.ConclusionsDeciphering if the EFD effects are dependent on PI4K inhibition, and/or via inhibition of other off‐target kinases will require the generation of novel, more potent, and more specific PI4K inhibitors.
Micelle‐enhanced spectrofluorimetric method for the rapid determination of bronchodilator terbutaline and its prodrug bambuterol: application for content uniformity test
Authors: Nour‐Eldeen Deena A. , Omar Mahmoud A. , Mohamed Salwa Kh. , Mohamed Abobakr A. 
Published: April 2022

AbstractA novel, simple and sensitive spectrofluorimetric approach for determination of terbutaline sulphate (TER) and its prodrug bambuterol (BAM) in their pure and pharmaceutical dosage forms was developed. The suggested approach depends on enhancing the native fluorescence of either TER or BAM at 315 and 297.2 nm after excitation at 277 and 259 nm, respectively, using sodium dodecyl sulphate (SDS) as a micellar medium. In the presence of 0.7% w/v SDS, ~1.38‐fold and 1.18‐fold enhancement is achieved in the relative fluorescence intensity (RFI) of TER and BAM, respectively. The fluorescence–concentration curves were rectilinear over the concentration range 0.8–16 μg ml−1, with detection limits (LOD) of 0.252 and 0.26 (μg ml−1), quantitation limits (LOQ) of 0.76 and 0.79 (μg ml−1), determination coefficients (r2) of 0.9981, and slopes of 45.92 and 10.44 for TER and BAM, respectively. The suggested approach was validated in accordance with International Council for Harmonisation criteria and was effectively applied in the analysis of the studied drugs in their commercial tablets. The high sensitivity of the proposed approach allows its application in evaluating the content uniformity testing of the studied drugs in their tablets through using the official United States Pharmacopeia criteria. Statistical analogies of the findings with that of the reported methods showed really good harmony and indicated no major differences in precision and accuracy.
Gulo gene locus, a new gene editing locus for mammalian cells
Authors: Habu Toshiyuki , Ishikawa Honoka , Kim Jiyeong 
Published: April 2022

AbstractTransgene technology is valuable and helpful in many fields, including basic medical and biological research, biotechnology, and therapy. Recent advances in targeting technology accelerate the production of transgenic plants and animals and the application for gene therapy. To develop the technology, we examine the utility as the new safe harbor locus, l‐gulono‐γ‐lactone oxidase (Gulo) locus in human and mice. We performed experiments in vitro and in vivo knockout and knockin mouse and cell lines to validate their applicability using these loci. The Gulo locus might be good candidates for safe harbor loci for transgenic research.
Visible Light‐Driven Carboxylation of Olefins by Using 2D Metal‐Free Covalent Organic Framework as Intrinsic Photocatalyst: A Sustainable Approach for CO2 Utilization
Authors: Sarkar Priyanka , Das Anjan , Ghosh Swarbhanu , Manirul Islam Sk. 
Published: April 2022

AbstractPhotocatalytic metal‐free carboxylation of olefins offers an environment‐friendly approach for resolving the increasing energy issue as well as mitigating the dilemma caused by the greenhouse effect. Carboxylation of styrene and its derivatives by photocatalytic CO2 reduction reaction shows great potential for sustainable utilization of greenhouse gas CO2 into valuable chemicals. Herein, we have constructed a highly crystalline and thermally stable 2D porous covalent organic framework (COF) having very low band‐gap energy, 1.8 eV and it shows efficient photocatalytic activity towards the carboxylation of aryl‐alkenes in good yields in presence of p‐terphenyl as a co‐catalyst at ambient temperature under visible light irradiation and 1 atmospheric CO2 pressure which facilitates to overcome the current transition metal catalyzed approaches. In addition, TR‐OT COF is a proficient and highly selective catalyst with outstanding recyclability. This suggests that this COF material is a novel photocatalyst for CO2 reduction towards α, β‐carboxylation of styrene and its derivatives under 20 W light‐emitting diode (LED) light.
Materials Formed by Combining Inorganic Glasses and Metal‐Organic Frameworks
Authors: Chester Ashleigh M. , Castillo‐Blas Celia , Wondraczek Lothar , Keen David A. , Bennett Thomas D. 
Published: April 2022

AbstractHere, we propose the combination of glassy or crystalline metal‐organic frameworks (MOFs) with inorganic glasses to create novel hybrid composites and blends.The motivation behind this new composite approach is to improve the processability issues and mechanical performance of MOFs, whilst maintaining their ubiquitous properties. Herein, the precepts of successful composite formation and pairing of MOF and glass MOFs with inorganic glasses are presented. Focus is also given to the synthetic routes to such materials and the challenges anticipated in both their production and characterisation. Depending on their chemical nature, materials are classified as crystalline MOF‐glass composites and blends. Additionally, the potential properties and applications of these two classes of materials are considered, the key aim being the retention of beneficial properties of both components, whilst circumventing their respective drawbacks.
A New Look at Molecular and Electronic Structure of Homoleptic Diiron(II,II) Complexes with N,N‐Bidentate Ligands: Combined Experimental and Theoretical Study
Authors: Korona Krzesimir , Terlecki Michał , Justyniak Iwona , Magott Michał , Żukrowski Jan , Kornowicz Arkadiusz , Pinkowicz Dawid , Kubas Adam , Lewiński Janusz 
Published: April 2022

AbstractPaddlewheel‐type binuclear complexes featuring metal−metal bonding have been the subject of widespread interest due to fundamental concern in their electronic structures and potential applications. Here, we explore the molecular and electronic structures of diiron(II,II) complexes with N,N’‐diarylformamidinate ligands. While a paddlewheel‐type diiron(II,II) complex with N,N’‐diphenylformamidinate ligands (DPhF) exhibits the centrosymmetric [Fe2(μ‐DPhF)4] structure, a minor alteration in the ligand system, i. e., switching from phenyl to p‐tolyl N‐substituted formamidinate ligand (DTolF), resulted in the isolation of an unprecedented non‐centrosymmetric [Fe(μ‐DTolF)3Fe(κ2‐DTolF)] complex. Both complexes were characterized using single‐crystal X‐ray diffraction, magnetic measurements, 57Fe Mössbauer spectroscopy, and cyclic voltammetry along with high‐level ab‐initio calculations. The results provide a new view on a range of factors controlling the ground‐state electronic configuration and structural diversity of homoleptic diiron(II,II) complexes. Model calculations determined that the Mayer bond orders for Fe−Fe interactions are significantly lower than 1 and equal to 0.15 and 0.28 for [Fe2(μ‐DPhF)4] and [Fe(μ‐DTolF)3Fe(κ2‐DTolF)], respectively.
PROTOCOL: Occupational health and safety regulatory interventions to improve the work environment: An evidence and gap map of effectiveness studies
Authors: Bondebjerg Anja , Filges Trine , Pejtersen Jan H. , Viinholt Bjørn C. A. , Burr Hermann , Hasle Peter , Tompa Emile , Birkefoss Kirsten , Bengtsen Elizabeth 
Published: April 2022

AbstractThis is the protocol for a Campbell systematic review. The objectives are as follows: provide an overview of the existing evidence base by identifying available systematic reviews and primary effectiveness studies, identify clusters of evidence suitable for a systematic review and identify gaps in evidence where primary research is needed.
PROTOCOL: Systematic review of methods to reduce risk of bias in knowledge translation interventional studies in health‐related issues
Authors: Ahmadi Ayat , Yazdizadeh Bahareh , Doshmangir Leila , Majdzadeh Reza , Asghari Shabnam 
Published: April 2022

AbstractBackgroundReview studies have reported on the low quality of study methodologies and poor reporting of knowledge translation (KT) interventional studies. This flaw cause the result of such studies to become misleading.ObjectivesThe present review is designed to evaluate the effect of methodological factors on the results of interventional studies that aimed to evaluate KT strategies at the policy level.Search MethodsBibliographic databases and grey literature databases will be searched. The retrieved studies will be recorded in Covidence. After screening titles and abstracts, the full texts of selected studies will be assessed against the inclusion criteria. Disagreements will be resolved through discussion or by consultation with a third author.Selection CriteriaPrimary studies are studies that aimed to estimate the efficacy of KT strategies to improve evidence‐informed policymaking. Study participants include policymakers and the intervention is a KT strategy. The main outcome is the desired changes in policy‐makers towards evidence‐informed decision‐making.Data Collection and AnalysisThe main effect sizes will be expressed as standard mean difference and its variance for the main efficacy outcome of KT strategies in primary studies. Forest plot meta‐analysis will be used to synthesize the effect of each group of KT strategies. The contribution of ROB to the efficacy of KT interventions will be assessed via Meta‐epidemiology analysis. The overall estimate will be calculated using inverse‐variance random‐effects meta‐analysis with a 95% confidence interval for the estimate.
Monosubstitution of 1H‐Imidazo[4,5‐f][1,10]phenanthroline Ligands Yields Maximum Luminescence Quantum Yield in Ruthenium Polypyridyl Complexes
Authors: Lämmle Martin , Bagemihl Benedikt , Nauroozi Djawed , Petermann Lydia , Pannwitz Andrea , Rau Sven 
Published: April 2022

AbstractSix different monoarylated N‐aryl‐1H‐imidazo[4,5‐f][1,10]phenanthroline (Rip) ligands and their corresponding ruthenium complexes [(tbbpy)2Ru(Rip)]2+ Ru(Rip) have been synthesized bearing different aryl substituents on the imidazole unit including electron donating and accepting groups. N,N’‐disubstitution on the imidazole unit yielded five new 1‐aryl‐3‐benzyl‐1H‐imidazo[4,5‐f][1,10]phenanthrolinium bromide (RR'ip)+ ligands and the corresponding ruthenium complexes [(tbbpy)2Ru(RR'ip)]3+ Ru(RR'ip). All complexes were characterized in terms of their structural, photophysical, photochemical and electrochemical properties. The photophysical data revealed a high luminescence quantum yield (21.6–22.8 %) for monoarylated Ru(Rip) complexes compared to [Ru(bpy)3]2+ (9.5 %) under inert conditions. Generating the imidazolium unit, yielding Ru(RR'ip)‐complexes lead to notable loss of quantum yields. Furthermore, we observed an enhanced photostability of Ru(RR'ip) complexes in the case of ortho substituted aryl groups.
Shikonin attenuates kidney tubular epithelial cells apoptosis, oxidative stress, and inflammatory response through nicotinamide adenine dinucleotide phosphate oxidase 4/PTEN pathway in acute kidney injury of sepsis model
Authors: Peng Yanqin , Li Yuan , Li Hao , Yu Junhua 
Published: April 2022

AbstractNatural compounds were used in the treatment of acute kidney injury (AKI) caused by sepsis. This study investigated the function of shikonin from the roots of Arnebia purpurea in sepsis‐induced AKI model. The target genes of shikonin were predicted by traditional Chinese medicine integrative database (TCMID). The markers of kidney injury, oxidative stress, and inflammatory factors were measured by enzyme‐linked immunosorbent assay (ELISA). The pathological changes of kidney tubules were assessed by Hematoxylin and Eosin staining. Apoptosis of kidney tubular epithelial cells (KTECs) was detected by the terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling. Protein expression was measured by western blot. Shikonin significantly improved kidney injury induced by cecal ligation and perforation (CLP). Besides, shikonin reduced KTECs apoptosis, malondialdehyde (MDA), reactive oxygen species (ROS), interleukin‐1β (IL‐1β), IL‐6, and tumor necrosis factor‐α (TNF‐α) levels, while augmented SOD and IL‐10 levels. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase4 (NOX4) was predicted a target gene of shikonin. The expression of NOX4 was significantly inhibited in shikonin‐treated group and the levels of phosphatidylinositol 3,4,5‐trisphosphate 3‐phosphate and dual specificity protein phosphate (PTEN) and p‐p65 were decreased, while level of p‐Akt was elevated. In vitro experiments, shikonin inhibited cell apoptosis, inflammatory, and ROS in human HK‐2 cells and rat TECs. Shikonin downregulated expression of NOX4, PTEN and p‐p65, and upregulated p‐AKT and Bcl‐2 expression in HK2 cells treated with lipopolysaccharide (LPS). Moreover, overexpression of NOX4 enhanced the effect of LPS on the expression level of PTEN, p‐p65, p‐AKT, and Bcl‐2, which was reversed by the addition of shikonin. Taken together, shikonin could improve sepsis‐induced AKI in rats, and attenuate the LPS induced KTECs apoptosis, oxidative stress, and inflammatory reaction via modulating NOX4/PTEN/AKT pathway.
Easy and convenient millimole‐scale synthesis of new, potential biomarkers for gamma‐hydroxybutyric acid (GHB) intake: Feasible for analytical laboratories
Authors: Steuer Christian , Quattrini Dario , Raeber Justine , Waser Philipp , Steuer Andrea E. 
Published: April 2022

AbstractNew biomarkers indicating the abuse of drugs and alcohol are still of major interest for clinical and forensic sciences. The endogenous neurotransmitter and approved drug, gamma‐hydroxybutyric acid (GHB), is often illegally used for drug‐facilitated crimes by spiking GHB into alcoholic beverages. Analytical detection windows of only 6 h in blood and 12 h in urine are often too short to provide reliable proof of GHB ingestion. Therefore, new biomarkers are needed to prove exogenous GHB administration. Previously, amino acid GHB conjugates were discovered in an untargeted metabolomics screening and fatty acid esters with GHB were recently discussed as promising biomarkers to enlarge the analytical detection time windows. However, the development of analytical methods is still slowed down since reference compounds for targeted screenings are still missing. In this paper, we describe simple procedures for the rapid synthesis and purification of amino acid GHB conjugates as well as fatty acid esters, which can be adopted in analytical and clinical/forensic laboratories. Structural characterization data, together with IR, 1H‐nuclear magnetic resonance (NMR), 13C‐NMR, high‐resolution mass spectra (MS), and MS/MS spectra in positive and negative ionization mode are reported for all obtained GHB conjugates and GHB conjugate precursors.
Food deprivation exposes sex‐specific trade‐offs between stress tolerance and life span in the copepod Tigriopus californicus
Authors: Li Ning , Flanagan Ben A. , Edmands Suzanne 
Published: April 2022

AbstractLong life is standardly assumed to be associated with high stress tolerance. Previous work shows that the copepod Tigriopus californicus breaks this rule, with longer life span under benign conditions found in males, the sex with lower stress tolerance. Here, we extended this previous work, raising animals from the same families in food‐replete conditions until adulthood and then transferring them to food‐limited conditions until all animals perished. As in previous work, survivorship under food‐replete conditions favored males. However, under food deprivation life span strongly favored females in all crosses. Compared to benign conditions, average life span under nutritional stress was reduced by 47% in males but only 32% in females. Further, the sex‐specific mitonuclear effects previously found under benign conditions were erased under food limited conditions. Results thus demonstrate that sex‐specific life span, including mitonuclear interactions, are highly dependent on nutritional environment.
Divergence in gut bacterial community between females and males in the wolf spider Pardosa astrigera
Authors: Gao Ying , Wu Pengfeng , Cui Shuyan , Ali Abid , Zheng Guo 
Published: April 2022

AbstractSex is one of the important factors affecting gut microbiota. As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males. However, how sex affects gut microbiota of spiders remains unclear. Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing. Results showed that the richness of bacterial microbiota in female spiders was significantly lower than in male spiders (p < .05). Besides, β‐diversity showed a significant difference between female and male spiders (p = .0270). The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05). The results also showed that amino acid and lipid metabolisms were significantly higher in female than in male spiders (p < .05), whereas glycan biosynthesis and metabolism were significantly higher in male than in female spiders (p < .05). Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.