bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANALISA KEWAJIBAN PAJAK PPN UNTUK MENILAI KEPATUHAN PAJAK PADA SALAH SATU BADAN NASIONAL MILIK PEMERINTAH
Authors: . Hasanudin 
Published: December 2022

Penelitian dilakukan di salah satu badan pemerintah, sebuah badan atau lembaga dalam bidang penyelesaian sengketa. Penelitian ini dilakukan guna menguji kesesuaian penerapan dan pelaporan kewajiban PPN. Data dikumpulkan melalui wawancara, dokumentasi dan studi kepustakaan untuk kemudian dianalisa dengan menggunakan metode deskriptif kualitatif. Analisa meliputi Pencatatan, Perhitungan dan Penyetoran Pajak penjualan, selanjutnya dalam hal pengisian SPT Masa Pajak Pertambahan Nilai sampai dengan pelaporannya. Adapun hasil yang diperoleh menunjukkan bahwa penerapan Pajak Pertambahan Nilai dan pelaporannya secara keseluruhan telah sesuai dengan aturan yang berlaku yaitu UU Nomor 42 Tahun 2009, akan tetapi ada kelalaian dalam penyetoran dan pelaporan yang terlambat pada periode tertentu sehingga bisa mempengaruhi kepatuhan Wajib Pajak. Hal ini disebabkan karena minimnya pemahaman dan ketelitian dalam sistem penerapan dan meningkatkan kesadaran dalam pelaporan Pajak Pertambahan.
Kata kunci: Penerapan dan Pelaporan, Pajak Pertambahan Nilai, Tingkat Kepatuhan Wajib
Penerapan Augmented Reality untuk Informasi Denah Kampus Institut Teknologi Garut
Authors: Latifah Ayu , Satria Eri , Hermawan Ari 
Published: December 2022

Penempatan berbagai ruangan yang terdapat di-kampus Institut Teknologi Garut tersusun dengan rapih dengan jumlah ruangan yang tidak sedikit, dengan terbatasnya media informasi alokasi penempatan ruangan, penulis memutuskan mengangkat masalah informasi alokasi ruang agar dapat di-visualisasikan ke dalam aplikasi android dan dapat ditampilkan secara virtual pada smartphone android. Penelitian ini bertujuan membuat kampus virtual dan dapat di-eksplor oleh user dengan menggunakan smartphone android. Penelitian ini menggunakan metodologi Research and Development (RnD) dalam tahapan pembuatan aplikasi dan menerapkan pengembangan ADDIE dengan tahapan analyze, design, develop, implement dan evaluate teknologi augmented reality dan dipadukan dengan sowftware Unity 3D dalam proses pembuatan aplikasi. Penelitian ini menghasilkan aplikasi kampus virtual yang dapat digunakan oleh civitas akademik maupun mahasiswa dalam mencari alokasi ruangan yang dicari dengan menggunakan smartphone android, adapun fitur utama dalam aplikasi ini adalah meng-eksplorasi bagian-bagian kampus. Diharapkan aplikasi yang dibuat ini dapat berlanjut dengan pengembangan berbagai fitur baru dalam proses eksplorasi maupun fitur teleportasi untuk mempermudah user dalam mencari ruangan sesuai nama ruangan tersebut.
Analisa Algoritma Association Rule pada Calon Mahasiswa Program Magister di masa Pandemi dengan Metode FP-Growth
Authors: M.hafizh  , Gushelmi  , Triana Novita  , Selvi Zola Fenia  , Emil Naf’an  
Published: December 2022

Pada masa sulit ini (pandemi) berdampak juga pada dunia pendidikan tekusus pada mereka yang ingin melanjutkan pendidikan tapi terhalang dan hilang semangat di karenakan kondisi pandemi.sehingga hampir setiap kampus merasakan sedikitnya mahasiswa baru yang mendaftar.karena hal tersebut sangat di butuhkan analisa yang mampu memberikan acuan dalam menyusun strategi dalam meningkatkan kembali jumlah orang yang mendaftar.slaah satu cara yang dapat dilaukan adalal dengan teknik dataminig, Adapun teknik yang digunakan dalam hal ini adalah Algoritma Association Rule metode FP-Growth. FP-Growth adalah salah satu alternatif algoritma yang dapat digunakan untuk menentukan himpunan data yang paling sering muncul (frequent itemset) dalam sekumpulan data. Algoritma FP-Growth merupakan pengembangan dari algoritma Apriori. Sedangkan di dalam algoritma FP-Growth tidak dilakukan generate candidate karena FP-Growth menggunakan konsep pembangunan tree dalam pencarian frequent itemset. Penelitian dilakukan dengan menganalisa fariabel data calon mahasiswa yang nantinya akan menghasilakn rule yang berguna sebagai acuan dalam mengambil keputusan.
EFFECT OF HYGIENE SANITATION AND PROMOTION ON PURCHASE DECISIONS AT FAVELA SUNSET CAFE BOGOR
Authors: Ishak Riani P. , Rabbania Faisal 
Published: December 2022

Cleanliness and sanitation are the most important things that must be considered to ensure that the products served to customers are safe and free from various viruses and bacteria. These issues, Hygiene Sanitation, and promotion must be considered by food and beverage entrepreneurs to increase the interest of buyers. The purpose of this study was to determine whether or not Hygiene Sanitation and Promotion influenced the purchase decisions at Favela Sunset Cafe Paledang, Bogor. The research method used is quantitative research. The data analysis used in this study consisted of multiple linear regression analysis with SPSS V 20.0. The result of the study concluded that the Effect of Hygiene Sanitation and Promotion on Purchase Decisions at Favela Sunset Cafe Paledang Bogor had a positive and significant influence.
Effect of sphincter of Oddi dysfunction on the abundance of biliary microbiota (biliary microecology) in patients with common bile duct stones
Authors: Liu Linxun , Zhao Zhanxue , Hou Xiaofan , Wu Jindu 
Published: December 2022

ObjectiveBiliary calculi, a common benign disease of the gastrointestinal tract, are affected by multiple factors, including diet, lifestyle, living environment, and personal and genetic background. Its occurrence is believed to be related to a change in biliary microbiota. Approximately 10%–20% of symptomatic patients with cholecystolithiasis have choledocholithiasis, resulting in infection, abdominal pain, jaundice, and biliary pancreatitis. This study aimed to determine whether a dysfunction in the sphincter of Oddi, which controls the outflow of bile and separates the bile duct from the intestine, leads to a change in biliary microbiota and the occurrence of biliary calculi.MethodsForty patients with cholecystolithiasis and choledocholithiasis were prospectively recruited. Bile specimens were obtained, and biliary pressure was measured during and after surgery. The collected specimens were analyzed with 16S rRNA gene to characterize the biliary microbiota. The risk factors of common bile duct calculi were analyzed numerically combined with the pressure in the sphincter of Oddi.ResultsDifferent biliary microbiota were found in all cases. Patients with sphincter of Oddi dysfunction had significantly increased biliary microbiota as well as significantly higher level of systemic inflammation than patients with normal sphincter of Oddi.ConclusionsThe systemic inflammatory response of patients with sphincter of Oddi dysfunction is more severe, and their microbial community significantly differs from that of patients with normal sphincter of Oddi, which makes biliary tract infection more likely; furthermore, the biliary tract of patients with sphincter of Oddi dysfunction has more gallstone-related bacterial communities.
Circulating fibroblast activation protein α is reduced in acute ischemic stroke
Authors: Sieweke Jan-Thorben , Grosse Gerrit M. , Weissenborn Karin , Derda Anselm A. , Biber Saskia , Bauersachs Johann , Bavendiek Udo , Tillmanns Jochen 
Published: December 2022

BackgroundFibroblast activation protein α (FAP), a membrane glycoprotein with dipeptidyl-peptidase and collagenase properties, is expressed in atherosclerotic plaques and remodeling of the extracellular matrix based on fibrosis. Fibrosis is a main contributor of atrial cardiomyopathies. In acute MI, circulating FAP is associated with outcome. Here, we investigated the correlation of circulating FAP to echocardiographic parameters of atrial remodeling and neurological impairment in acute ischemic stroke.MethodsCirculating FAP plasma concentrations were determined by ELISA in 47 patients with acute stroke and 22 control patients without stroke. Echocardiography was performed in all participants. Laboratory analysis, National Institutes of Health Stroke Scale (NIHSS) scoring and prolonged Holter-ECG-monitoring were performed in all stroke patients.ResultsPatients with acute stroke had lower circulating FAP concentrations than the control cohort (92 ± 24 vs. 106 ± 22 ng/mL, P &lt; 0.001). There was no difference between the circulating FAP concentration comparing stroke due to atrial fibrillation, embolic stroke of undetermined source (ESUS) or atherosclerotic origin. Septal atrial conduction time (sPA-TDI) and left atrial (LA) volume index to tissue Doppler velocity (LAVI/a‘) representing echocardiographic parameters of LA remodeling did not correlate with FAP concentrations (sPA-TDI: r = 0.123, p = 0.31; LAVI/a‘: r = 0.183, p = 0.132). Stroke severity as assessed by NIHSS inversely correlated with circulating FAP (r = −0.318, p = 0.04). FAP concentration had a fair accuracy for identifying stroke in the receiver operating characteristic (ROC) analysis (AUC = 0.710, 95% CI: 0.577–0.843). A FAP concentration of 101 ng/mL discriminated between presence and absence of stroke with a sensitivity of 72% and a specificity of 77%. Lower circulating FAP concentration was associated with cardio-cerebro-vascular events within 12 months after admission.ConclusionsOur study is the first to associate FAP with echocardiographic parameters of LA-remodeling and function. FAP did not correlate with sPA-TDI and LAVI/a‘. However, FAP was associated with stroke, neurological impairment, and cardio-cerebral events within 12 months. Therefore, FAP might enable individualized risk stratification in ischemic stroke.
CVD risk in non-albuminuric chronic kidney disease in hypertensive, non-diabetic subjects: A post-hoc analysis from SPRINT
Authors: Sheng Chang-Sheng , Wang Dan , Yuan Jiangzi , Cheng Yi , Sun Siming , Yang Yulin , Miao Ya , Wang Weiming , Tian Jingyan , Bloomgarden Zachary T. 
Published: December 2022

IntroductionThe risks associated with non-albuminuric chronic kidney disease (CKD) have been investigated in diabetes mellitus but not in hypertensive patients. The objective of this study was to investigate the risks associated with non-albuminuric CKD in treated hypertensive patients in the Systolic Blood Pressure Intervention Trial (SPRINT) population.MethodsBased on baseline albuminuria status (urine albumin/creatinine ratio [UACR], ≥30 or &lt;30 mg/g) and the levels of estimated glomerular filtration rate ([eGFR], ≥60, 45–59, or &lt;45 mL/min/1.73 m2), participants were classified into six subgroups to assess the risks associated with the primary outcome and mortality. The primary composite outcome was myocardial infarction, other acute coronary syndromes, stroke, heart failure, or mortality from cardiovascular causes.ResultsDuring a median follow-up of 3.26 years in 8,866 hypertensive patients, there were 352 deaths and 547 participants with the primary outcome. In adjusted Cox regression analysis using non-CKD and non-albuminuria (eGFR ≥60 mL/min/1.73 m2 combined with UACR &lt;30 mg/g) as reference, albuminuria whether combined with CKD or not, showed significantly higher risk of both primary outcome and all-cause mortality in the total population. Whereas, non-albuminuria only combined with eGFR &lt;45 mL/min/1.73 m2 showed significantly higher risk of both primary outcome and all-cause mortality in the intensive-therapy group.DiscussionNon-albuminuric CKD did have higher risk of all-cause and CVD mortality only if the eGFR &lt;45 mL/min/1.73 m2. Increased albuminuria conferred higher risk of primary outcome and all-cause mortality irrespective the levels of eGFR.Clinical trial registrationClinicalTrials.gov, number: NCT01206062.
Identification of B cell marker genes based on single-cell sequencing to establish a prognostic model and identify immune infiltration in osteosarcoma
Authors: Zhang Zhongmin , Zhang Jin , Duan Yuansheng , Li Xuesong , Pan Jie , Wang Guowen , Shen Bin 
Published: December 2022

BackgroundTumor-infiltrating B cells play a crucial role in the promotion or inhibition of tumor development. However, the role of B cells in osteosarcoma remains largely unknown. The aim of this study was to investigate the effect of B cells on the prognosis and immunity infiltration of osteosarcoma.MethodsMarker genes of B cells were identified based on the single-cell sequencing results of osteosarcoma in the GEO database. The prognostic model was established by the TCGA database and verified by the GEO data. The divergence in immune infiltration between the low-risk and high-risk groups was then compared according to the established prognostic model. Finally, the differential genes in the low-risk and high-risk groups were enriched and analyzed.ResultsA total of 261 B cell marker genes was obtained by single-cell sequencing and a prognostic model of 4 B cell marker genes was established based on TCGA data. The model was found to have a good prediction performance in the TCGA and GEO data. A remarkable difference in immune infiltration between the low-risk and high-risk groups was also observed. The obtained results were verified by enrichment analysis.ConclusionIn summary, a prognostic model with good predictive performance was established that revealed the indispensable role of B cells in the development of osteosarcoma. This model also provides a predictive index and a novel therapeutic target for immunotherapy for clinical patients.
Endoscopic transpapillary drainage for walled-off pancreatic necrosis with complete main pancreatic duct disruption by metallic stent placement: A retrospective study
Authors: Meng Yajie , Ding Jiewen , Tian Chuan , Wang Min , Tang Kejiang 
Published: December 2022

BackgroundWalled-off pancreatic necrosis (WOPN) is a serious complication of acute necrotizing pancreatitis (ANP) and may lead to disruption of the main pancreatic duct (MPD). Endoscopic passive transpapillary drainage (PTD) is an effective method for treating MPD disruptions. However, WOPN with complete MPD disruption is usually accompanied by disconnected pancreatic duct syndrome (DPDS), especially with infected necrosis. Endoscopic PTD with a fully covered self-expanding metallic stent (FCSEMS) and a plastic stent placement may have the potential for future application in treating complete MPD disruption in patients with WOPN.MethodsPatients with WOPN caused by ANP were classified according to the 2012 Atlanta classification and definition. In all patients, ERCP was performed 2 times. First, 3 patients were diagnosed with complete MPD disruption by ERCP. At the time of diagnosis, a plastic pancreatic stent (7Fr) was placed. Second, they underwent endoscopic PTD for WOPN with complete MPD disruption in which an FCSEMS and plastic stent placement were the only access routes to the necrotic cavity.ResultsThe etiology of pancreatitis in these patients was of biliary, lipogenic, and alcoholic origin. The WOPN lesion size ranged from 6.5 to 10.2 cm in this study, and the type of WOPN was mixed in two cases and central in one case. The type of MPD disruption was complete in all three patients. The locations of disruption included the pancreatic body and head. The time from occurrence to the first ERCP was 18, 23, and 26 days, respectively. The main symptoms were abdominal pain, abdominal distention, fever, gastrointestinal obstruction, and/or weight loss. The three patients with symptomatic WOPN and MPD disruption underwent endoscopic PTD with FCSEMS and plastic pancreatic stent placement. Technical and therapeutic successes were achieved in 3/3 of patients. The mean time of stenting was 28–93 days. The clinical symptoms connected with WOPN and collection disappeared postoperatively in all three patients. During the follow-up period of 4–18 months, no patient developed collection recurrence or other complications, such as gastrointestinal bleeding or reinfection. All patients recovered uneventfully.ConclusionIn patients with WOPN with complete MPD disruption, endoscopic PTD with FCSEMSs and plastic stent placement may be an effective and safe method of treatment.
Diagnostic efficiency of metagenomic next-generation sequencing for suspected spinal tuberculosis in China: A multicenter prospective study
Authors: Li Yuan , Yao Xiao-wei , Tang Liang , Dong Wei-jie , Lan Ting-long , Fan Jun , Liu Feng-sheng , Qin Shi-bing 
Published: December 2022

BackgroundThe pathogens of suspected spinal tuberculosis (TB) include TB and non-TB bacteria. A rapid and effective diagnostic method that can detect TB and non-TB pathogens simultaneously remains lacking. Here, we used metagenomic next-generation sequencing (mNGS) to detect the pathogens in patients with suspected spinal TB.MethodsThe enrolled patients with suspected spinal TB were regrouped three times into patients with spinal infection and controls, patients with spinal TB and controls, and patients with non-TB spinal infection and controls. We tested the three groups separately by using mNGS and conventional detection methods.ResultsUltimately, 100 patients were included in this study. Pathogens were detected in 82 patients. Among the 82 patients, 37 had TB and 45 were infected with other bacteria. In patients with spinal infection, the sensitivity of the mNGS assay was higher than that of culture and pathological examination (p &lt; 0.001, p &lt; 0.001). The specificity of the mNGS assay was not statistically different from that of culture and pathological examination (p = 1.000, p = 1.000). In patients with spinal TB, no statistical difference was found between the sensitivity of the mNGS assay and that of Xpert and T-SPOT.TB (p = 1.000, p = 0.430). The sensitivity of the mNGS assay was higher than that of MGIT 960 culture and pathological examination (p &lt; 0.001, p = 0.006). The specificities of the mNGS assay, Xpert, MGIT 960 culture, and pathological examination were all 100%. The specificity of T-SPOT.TB (78.3%) was lower than that of the mNGS assay (100%; p &lt; 0.001). In patients with non-TB spinal infection, the sensitivity of the mNGS assay was higher than that of bacterial culture and pathological examination (p &lt; 0.001, p &lt; 0.001). The specificity of the mNGS assay was not statistically different from that of bacterial culture and pathological examination (p = 1.000, p = 1.000).ConclusionData presented here demonstrated that mNGS can detect TB and non-TB bacteria simultaneously, with high sensitivity, specificity and short detection time. Compared with conventional detection methods, mNGS is a more rapid and effective diagnostic tool for suspected spinal TB.
Construction and analysis of gene co-expression network in the pathogenic fungus Ustilago maydis
Authors: Soberanes-Gutiérrez Cinthia V. , Castillo-Jiménez Alfredo , Pérez-Rueda Ernesto , Galán-Vásquez Edgardo 
Published: December 2022

IntroductionBiological systems respond to environmental disturbances and a wide range of compounds through complex gene interaction networks. The enormous growth of experimental information obtained using large-scale genomic techniques such as microarrays and RNA sequencing led to the construction of a wide variety of gene co-expression networks in recent years. These networks allow the discovery of clusters of co-expressed genes that potentially work in the same process linking them to biological processes often of interest to industrial, medicinal, and academic research.MethodsIn this study, we built the gene co-expression network of Ustilago maydis from the gene expression data of 168 samples belonging to 19 series, which correspond to the GPL3681 platform deposited in the NCBI using WGCNA software. This network was analyzed to identify clusters of co-expressed genes, gene hubs and Gene Ontology terms. Additionally, we identified relevant modules through a hypergeometric approach based on a predicted set of transcription factors and virulence genes.Results and DiscussionWe identified 13 modules in the gene co-expression network of U. maydis. The TFs enriched in the modules of interest belong to the superfamilies of Nucleic acid-binding proteins, Winged helix DNA-binding, and Zn2/Cys6 DNA-binding. On the other hand, the modules enriched with virulence genes were classified into diseases related to corn smut, Invasive candidiasis, among others. Finally, a large number of hypothetical, a large number of hypothetical genes were identified as highly co-expressed with virulence genes, making them possible experimental targets.
The effect of Lactobacillus with prebiotics on KPC-2-producing Klebsiella pneumoniae
Authors: Tang Hung-Jen , Chen Chi-Chung , Lu Ying-Chen , Huang Hui-Ling , Chen Hung-Jui , Chuang Yin-Ching , Lai Chih-Cheng , Chao Chien-Ming 
Published: December 2022

ObjectivesThis study investigated the inhibitory effect ofLactobacillusspp. with prebiotics againstKlebsiella pneumoniaecarbapenemase-2 (KPC-2)-producingKlebsiella pneumoniaeusing bothin vitroexperiments and animal models.MethodsThirty-threeLactobacillusspp. strains were confirmed by 16S rDNA sequencing, and four different PFGE genotyped KPC-2-producingK. pneumoniaestrains were selected for investigation.In vitrostudies, including broth microdilution assays, changes in pH values in lactobacilli cultures with different prebiotics, time-kill tests ofLactobacillusspp. against KPC-2-producingK. pneumoniaeand furtherin vivo Lactobacillusalone or in combination with prebiotics against KPC-2-producingK. pneumoniaein an animal model, were performed.ResultsThe lower pH value of the cell-free supernatant was associated with a lower minimal inhibitory percentage of theLactobacillusstrain against KPC-2-producingK. pneumoniae.Furthermore, lactulose/isomalto-oligosaccharide/inulin and fructo-oligosaccharide can enhance the inhibitory effect of all 107CFU/mlLactobacillusstrains against KPC001. ThreeLactobacillusstrains (LYC1154, LYC1322, and LYC1511) that could be persistently detected in the stool were tested for their ability to reduce the amount of KPC001 in the feces individually or in combination. A significantly better effect in reducing the amount of KPC001 was observed for the combination of three differentLactobacillusspecies than for each of them alone. Furthermore, their inhibitory effect was enhanced after adding lactulose or isomalto-oligosaccharide (bothp &lt; 0.05).ConclusionThis study demonstrates the inhibitory effect of probioticLactobacillus, including LYC1154, LYC1322, and LYC1511, with prebiotics such as lactulose or isomalto-oligosaccharide against the colonization of KPC-2-producingK. pneumoniae.