bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pollination by the hoverflyEristalinus aeneus(Diptera: Syrphidae) in two hybrid seed crops: celery and fennel (Apiaceae)
Authors: Sánchez M. , Belliure B. , Montserrat M. , Gil J. , Velásquez Y. 
Published: June 2022

AbstractHybrid seed production often relies on managed pollinators whose use in isolation cages can pose challenges. Here, we evaluated the pollination ability of the novel managed pollinatorEristalinus aeneus(Diptera: Syrphidae) in hybrid celery and fennel seed crops. During trials performed in 2019–2020 in Cordoba, Spain, we compared seed production with hoverflies released at high and low densities (40–20 ind./m2), and without released pollinators. In celery, we included a treatment withLucilia sericata(Diptera: Calliphoridae), a blowfly used for seed production (375 ind./m2). The production of celery seeds whenE. aeneuswere released at high density was significantly higher (+49%) than without released pollinators, for both sterile and fertile plant genotypes; no significant differences were found between the two hoverfly densities and the blowfly treatments. In fennel, seed yield of fertile plants with high density of hoverflies was higher than with low density (+149%), or without released pollinators (+168%); whereas for sterile plants no differences were found between treatments. In both crops and plant genotypes, the highest pollen adhesion to stigmas (number of pollen grains and proportion of flowers with pollen adhered in two stigmas) was obtained with hoverflies at high density. In celery, pollen adhesion was higher with high density of hoverflies than with blowflies for both plant genotypes, despite the considerably lower numbers of released hoverflies. Our results suggest thatE. aeneusis a more efficient pollinator of celery thanL. sericata, and that it has the potential to effectively pollinate other hybrid Apiaceae seed crops like fennel.
The incidence of Orientia tsutsugamushi infection in rural South India
Authors: Devamani Carol S. , Prakash John A. J. , Alexander Neal , Stenos John , Schmidt Wolf-Peter 
Published: June 2022

Abstract
Scrub typhus is a common bacterial infection in Asia caused by Orientia tsutsugamushi. This serological cohort study estimated the incidence of infection in a rural population in South India. Participants were enrolled through systematic sampling in 46 villages at baseline, and revisited the following year. Blood samples were tested for IgG antibodies using ELISA, followed by indirect immunofluorescence assays (IFA) in those positive for ELISA at both rounds. A case was defined as sero-conversion (ELISA), or at least a 4-fold titre increase (IFA), between the two time points. In addition to crude incidence rate estimates, we used piecewise linear rates across calendar months, with rates proportional to the monthly incidence of local hospital cases to address seasonality and unequal follow-up times. Of 402 participants, 61.7% were female. The mean age was 46.7 years, (range 13–88). 21 participants showed evidence for serological infection. The estimated incidence was 4.4 per 100 person-years (95% CI 2.8–6.7). The piecewise linear rates approach resulted in a similar estimate of 4.6 per 100 person years (95% CI 2.9–6.9). Considering previous estimates of symptomatic scrub typhus incidence in the same study population, only about 2–5% of infections may result in clinically relevant disease.
Evidence of steep declines in the heavily traded Javan White-eyeZosterops flavusfrom repeated standardised surveys
Authors: van Balen S. (Bas) , Saryanthi Ria , Marsden Stuart 
Published: June 2022

SummaryMany Indonesian, and in particular Javan, birds are suspected to have declined dramatically as a result of unsustainable trapping for the cagebird trade, but quantitative evidence of the scale of declines is lacking for the great majority of species. We conducted field surveys of the heavily traded Javan White-eyeZosterops flavusat 19 key sites in 2018–2019 matching the methods and personnel used in baseline surveys done around 10 years earlier. Overall numbers counted were 84% lower in the later survey, and while more white-eyes were recorded at three sites in 2018–2019, there was a significant decline in numbers across all sites. The three sites with highest numbers in 2006–2019 (502 birds counted) had 22 individuals counted in 2018–2019, but there was no overall trend for ‘declines’ to be greater at sites that held more birds originally. Declines in white-eyes were much steeper than those of several lesser-traded bird species at the sites, suggesting that trapping has been a more important driver of declines than habitat changes such as conversion of mangrove to shrimp ponds. Small numbers of white-eyes were recorded at several previously unvisited sites, but we suggest that the species, on Java at least, has shown declines in the region of 80% over the last 10 years. Although since 2018 Javan White-eye is legally protected, we urge that this protection is extended to all white-eye species, because of their similarity.
Algorithmic Differentiation for Sensitivity Analysis in Cloud Microphysics
Authors: Hieronymus M. , Baumgartner M. , Miltenberger A. , Brinkmann A. 
Published: June 2022

AbstractThe role of clouds for radiative transfer, precipitation formation, and their interaction with atmospheric dynamics depends strongly on cloud microphysics. The parameterization of cloud microphysical processes in weather and climate models is a well‐known source of uncertainties. Hence, robust quantification of this uncertainty is mandatory. Sensitivity analysis to date has typically investigated only a few model parameters. We propose algorithmic differentiation (AD) as a tool to detect the magnitude and timing at which a model state variable is sensitive to any of the hundreds of uncertain model parameters in the cloud microphysics parameterization. AD increases the computational cost by roughly a third in our simulations. We explore this methodology as the example of warm conveyor belt trajectories, that is, air parcels rising rapidly from the planetary boundary layer to the upper troposphere in the vicinity of an extratropical cyclone. Based on the information of derivatives with respect to the uncertain parameters, the ten parameters contributing most to uncertainty are selected. These uncertain parameters are mostly related to the representation of hydrometeor diameter and fall velocity, the activation of cloud condensation nuclei, and heterogeneous freezing. We demonstrate the meaningfulness of the AD‐estimated sensitivities by comparing the AD results with ensemble simulations spawned at different points along the trajectories, where different parameter settings are used in the various ensemble members. The ranking of the most important parameters from these ensemble simulations is consistent with the results from AD. Thus, AD is a helpful tool for selecting parameters contributing most to cloud microphysics uncertainty.
Therapy response of glucocorticoid-refractory acute GVHD of the lower intestinal tract
Authors: Biavasco Francesca , Ihorst Gabriele , Wäsch Ralph , Wehr Claudia , Bertz Hartmut , Finke Jürgen , Zeiser Robert 
Published: June 2022

AbstractAcute graft-versus-host disease (aGVHD) is a major life-threatening complication of allogeneic hematopoietic cell transplantation. While most studies report therapy-response of aGVHD including a cumulative grade of skin, liver and intestinal tract manifestations, there is a lack of information specifically on lower gastrointestinal tract aGVHD (GI-GVHD) therapy-response, which is highly relevant in light of novel therapies that target intestinal regeneration such as IL-22, R-spondin or GLP-2. Here we retrospectively analyzed patients who developed GI-GVHD over a 6-year period. A total of 144 patients developed GI-GVHD and 82 (57%) were resistant to glucocorticoid-therapy (SR). The most commonly used second-line therapy was ruxolitinib (74%). Overall and complete response (CR) to ruxolitinib on day 28 were 44.5% and 13%, respectively. SR-GVHD patients experienced a lower 5-year overall survival (OS) (34.8 vs 53.3%,p = 0.0014) and higher incidence of 12-months non-relapse-mortality (39.2 vs 14.3%,p = 0.016) compared to glucocorticoid-sensitive patients. SR-GI-GVHD patients, that achieved a CR on day 28 after ruxolitinib start, experienced a higher OS compared to non-CR patients (p = 0.04). These findings indicate that therapy response of SR-GI-GVHD to different immunosuppressive approaches is still low, and that novel therapies specifically aiming at enhanced intestinal regeneration should be tested in clinical trials.
Intracontinental deformation of the Tianshan Orogen in response to India-Asia collision
Authors: Li Wei , Chen Yun , Yuan Xiaohui , Xiao Wenjiao , Windley Brian F. 
Published: June 2022

AbstractHow the continental lithosphere deforms far away from plate boundaries has been long debated. The Tianshan is a type-example of ongoing lithospheric deformation in an intracontinental setting. It formed during the Paleozoic accretion of the Altaids and was rejuvenated in the Cenozoic, which might be a far-field response to the India-Asia collision. Here we present seismic images of the lithosphere across the central Tianshan, which were constructed from receiver functions and Rayleigh wave dispersions along a N–S-trending linear seismic array. We observe an extensively deformed lithosphere in the Tianshan with inherited, structurally controlled brittle deformation in the shallow crust and plastic deformation near the Moho. We find that earlier multiple accretionary structures were preserved in the crust, which was deformed by pure-shear shortening in the south and thick-skinned tectonics in the north but was limitedly underthrusted by surrounding blocks. A balanced cross-section of Moho discontinuities supports the concept that intracontinental deformation in the Tianshan intensified synchronously with the direct contact between the underthrusting Indian slab and the Tarim Craton in the Late Miocene (~10 Ma). These findings provide a robust and unified seismic model for the Tianshan Orogen, and confirm that effective delivery of the India-Asia collision stress induced the rejuvenation of this intracontinental orogen.
Passive mode-locking and terahertz frequency comb generation in resonant-tunneling-diode oscillator
Authors: Hiraoka Tomoki , Inose Yuta , Arikawa Takashi , Ito Hiroshi , Tanaka Koichiro 
Published: June 2022

AbstractOptical frequency combs in the terahertz frequency range are long-awaited frequency standards for spectroscopy of molecules and high-speed wireless communications. However, a terahertz frequency comb based on a low-cost, energy-efficient, and room-temperature-operating device remains unavailable especially in the frequency range of 0.1 to 3 THz. In this paper, we show that the resonant-tunneling-diode (RTD) oscillator can be passively mode-locked by optical feedback and generate a terahertz frequency comb. The standard deviation of the spacing between the comb lines, i.e., the repetition frequency, is reduced to less than 420 mHz by applying external bias modulation. A simulation model successfully reproduces the mode-locking behavior by including the nonlinear capacitance of RTD and multiple optical feedback. Since the mode-locked RTD oscillator is a simple semiconductor device that operates at room temperature and covers the frequency range of 0.1 to 2 THz (potentially up to 3 THz), it can be used as a frequency standard for future terahertz sensing and wireless communications.
Distinct nociception processing in the dysgranular and barrel regions of the mouse somatosensory cortex
Authors: Osaki Hironobu , Kanaya Moeko , Ueta Yoshifumi , Miyata Mariko 
Published: June 2022

AbstractNociception, a somatic discriminative aspect of pain, is, like touch, represented in the primary somatosensory cortex (S1), but the separation and interaction of the two modalities within S1 remain unclear. Here, we show spatially distinct tactile and nociceptive processing in the granular barrel field (BF) and adjacent dysgranular region (Dys) in mouse S1. Simultaneous recordings of the multiunit activity across subregions revealed that Dys neurons are more responsive to noxious input, whereas BF neurons prefer tactile input. At the single neuron level, nociceptive information is represented separately from the tactile information in Dys layer 2/3. In contrast, both modalities seem to converge on individual layer 5 neurons of each region, but to a different extent. Overall, these findings show layer-specific processing of nociceptive and tactile information between Dys and BF. We further demonstrated that Dys activity, but not BF activity, is critically involved in pain-like behavior. These findings provide new insights into the role of pain processing in S1.
Pervasive translation of circular RNAs driven by short IRES-like elements
Authors: Fan Xiaojuan , Yang Yun , Chen Chuyun , Wang Zefeng 
Published: June 2022

AbstractSome circular RNAs (circRNAs) were found to be translated through IRES-driven mechanism, however the scope and functions of circRNA translation are unclear because endogenous IRESs are rare. To determine the prevalence and mechanism of circRNA translation, we develop a cell-based system to screen random sequences and identify 97 overrepresented hexamers that drive cap-independent circRNA translation. These IRES-like short elements are significantly enriched in endogenous circRNAs and sufficient to drive circRNA translation. We further identify multiple trans-acting factors that bind these IRES-like elements to initiate translation. Using mass-spectrometry data, hundreds of circRNA-coded peptides are identified, most of which have low abundance due to rapid degradation. As judged by mass-spectrometry, 50% of translatable endogenous circRNAs undergo rolling circle translation, several of which are experimentally validated. Consistently, mutations of the IRES-like element in one circRNA reduce its translation. Collectively, our findings suggest a pervasive translation of circRNAs, providing profound implications in translation control.
A guided multiverse study of neuroimaging analyses
Authors: Dafflon Jessica , F. Da Costa Pedro , Váša František , Monti Ricardo Pio , Bzdok Danilo , Hellyer Peter J. , Turkheimer Federico , Smallwood Jonathan , Jones Emily , Leech Robert 
Published: June 2022

AbstractFor most neuroimaging questions the range of possible analytic choices makes it unclear how to evaluate conclusions from any single analytic method. One possible way to address this issue is to evaluate all possible analyses using a multiverse approach, however, this can be computationally challenging and sequential analyses on the same data can compromise predictive power. Here, we establish how active learning on a low-dimensional space capturing the inter-relationships between pipelines can efficiently approximate the full spectrum of analyses. This approach balances the benefits of a multiverse analysis without incurring the cost on computational and predictive power. We illustrate this approach with two functional MRI datasets (predicting brain age and autism diagnosis) demonstrating how a multiverse of analyses can be efficiently navigated and mapped out using active learning. Furthermore, our presented approach not only identifies the subset of analysis techniques that are best able to predict age or classify individuals with autism spectrum disorder and healthy controls, but it also allows the relationships between analyses to be quantified.
Diverse mantle components with invariant oxygen isotopes in the 2021 Fagradalsfjall eruption, Iceland
Authors: Bindeman I. N. , Deegan F. M. , Troll V. R. , Thordarson T. , Höskuldsson Á. , Moreland W. M. , Zorn E. U. , Shevchenko A. V. , Walter T. R. 
Published: June 2022

AbstractThe basalts of the 2021 Fagradalsfjall eruption were the first erupted on the Reykjanes Peninsula in 781 years and offer a unique opportunity to determine the composition of the mantle underlying Iceland, in particular its oxygen isotope composition (δ18O values). The basalts show compositional variations in Zr/Y, Nb/Zr and Nb/Y values that span roughly half of the previously described range for Icelandic basaltic magmas and signal involvement of Icelandic plume (OIB) and Enriched Mid-Ocean Ridge Basalt (EMORB) in magma genesis. Here we show that Fagradalsfjall δ18O values are invariable (mean δ18O = 5.4 ± 0.3‰ 2 SD, N = 47) and indistinguishable from “normal” upper mantle, in contrast to significantly lower δ18O values reported for erupted materials elsewhere in Iceland (e.g., the 2014–2015 eruption at Holuhraun, Central Iceland). Thus, despite differing trace element characteristics, the melts that supplied the Fagradalsfjall eruption show no evidence for 18O-depleted mantle or interaction with low-δ18O crust and may therefore represent a useful mantle reference value in this part of the Icelandic plume system.
Rhythmic interactions between the mediodorsal thalamus and prefrontal cortex precede human visual perception
Authors: Griffiths Benjamin J. , Zaehle Tino , Repplinger Stefan , Schmitt Friedhelm C. , Voges Jürgen , Hanslmayr Simon , Staudigl Tobias 
Published: June 2022

AbstractThe thalamus is much more than a simple sensory relay. High-order thalamic nuclei, such as the mediodorsal thalamus, exert a profound influence over animal cognition. However, given the difficulty of directly recording from the thalamus in humans, next-to-nothing is known about thalamic and thalamocortical contributions to human cognition. To address this, we analysed simultaneously-recorded thalamic iEEG and whole-head MEG in six patients (plus MEG recordings from twelve healthy controls) as they completed a visual detection task. We observed that the phase of both ongoing mediodorsal thalamic and prefrontal low-frequency activity was predictive of perceptual performance. Critically however, mediodorsal thalamic activity mediated prefrontal contributions to perceptual performance. These results suggest that it is thalamocortical interactions, rather than cortical activity alone, that is predictive of upcoming perceptual performance and, more generally, highlights the importance of accounting for the thalamus when theorising about cortical contributions to human cognition.
WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage
Authors: Han Seong-Su , Wen Kuo-Kuang , García-Rubio María L. , Wold Marc S. , Aguilera Andrés , Niedzwiedz Wojciech , Vyas Yatin M. 
Published: June 2022

AbstractPerturbation in the replication-stress response (RSR) and DNA-damage response (DDR) causes genomic instability. Genomic instability occurs in Wiskott-Aldrich syndrome (WAS), a primary immunodeficiency disorder, yet the mechanism remains largely uncharacterized. Replication protein A (RPA), a single-strand DNA (ssDNA) binding protein, has key roles in the RSR and DDR. Here we show that human WAS-protein (WASp) modulates RPA functions at perturbed replication forks (RFs). Following genotoxic insult, WASp accumulates at RFs, associates with RPA, and promotes RPA:ssDNA complexation. WASp deficiency in human lymphocytes destabilizes RPA:ssDNA-complexes, impairs accumulation of RPA, ATR, ETAA1, and TOPBP1 at genotoxin-perturbed RFs, decreases CHK1 activation, and provokes global RF dysfunction.las17(yeastWAS-homolog)-deficientS. cerevisiaealso show decreased ScRPA accumulation at perturbed RFs, impaired DNA recombination, and increased frequency of DNA double-strand break (DSB)-induced single-strand annealing (SSA). Consequently, WASp (or Las17)-deficient cells show increased frequency of DSBs upon genotoxic insult. Our study reveals an evolutionarily conserved, essential role of WASp in the DNA stress-resolution pathway, such that WASp deficiency provokes RPA dysfunction-coupled genomic instability.
Scanning gradiometry with a single spin quantum magnetometer
Authors: Huxter W. S. , Palm M. L. , Davis M. L. , Welter P. , Lambert C.-H. , Trassin M. , Degen C. L. 
Published: June 2022

AbstractQuantum sensors based on spin defects in diamond have recently enabled detailed imaging of nanoscale magnetic patterns, such as chiral spin textures, two-dimensional ferromagnets, or superconducting vortices, based on a measurement of the static magnetic stray field. Here, we demonstrate a gradiometry technique that significantly enhances the measurement sensitivity of such static fields, leading to new opportunities in the imaging of weakly magnetic systems. Our method relies on the mechanical oscillation of a single nitrogen-vacancy center at the tip of a scanning diamond probe, which up-converts the local spatial gradients into ac magnetic fields enabling the use of sensitive ac quantum protocols. We show that gradiometry provides important advantages over static field imaging: (i) an order-of-magnitude better sensitivity, (ii) a more localized and sharper image, and (iii) a strong suppression of field drifts. We demonstrate the capabilities of gradiometry by imaging the nanotesla fields appearing above topographic defects and atomic steps in an antiferromagnet, direct currents in a graphene device, and para- and diamagnetic metals.
SARS-CoV-2 antibody trajectories after a single COVID-19 vaccination with and without prior infection
Authors: Wei Jia , Matthews Philippa C. , Stoesser Nicole , Diamond Ian , Studley Ruth , Rourke Emma , Cook Duncan , Bell John I. , Newton John N. , Farrar Jeremy , Howarth Alison , Marsden Brian D. , Hoosdally Sarah , Jones E. Yvonne , Stuart David I. , Crook Derrick W. , Peto Tim E. A. , Walker A. Sarah , Eyre David W. , Pouwels Koen B. ,   The COVID-19 Infection Survey team, Thomas Tina , Ayoubkhani Daniel , Black Russell , Felton Antonio , Crees Megan , Jones Joel , Lloyd Lina , Sutherland Esther , Pritchard Emma , Vihta Karina-Doris , Doherty George , Kavanagh James , Chau Kevin K. , Hatch Stephanie B. , Ebner Daniel , Ferreira Lucas Martins , Christott Thomas , Dejnirattisai Wanwisa , Mongkolsapaya Juthathip , Cameron Sarah , Tamblin-Hopper Phoebe , Wolna Magda , Brown Rachael , Cornall Richard , Screaton Gavin , Lythgoe Katrina , Bonsall David , Golubchik Tanya , Fryer Helen , Cox Stuart , Paddon Kevin , James Tim , House Thomas , Robotham Julie , Birrell Paul , Jordan Helena , Sheppard Tim , Athey Graham , Moody Dan , Curry Leigh , Brereton Pamela , Jarvis Ian , Godsmark Anna , Morris George , Mallick Bobby , Eeles Phil , Hay Jodie , VanSteenhouse Harper , Lee Jessica , White Sean , Evans Tim , Bloemberg Lisa , Allison Katie , Pandya Anouska , Davis Sophie , Conway David I. , MacLeod Margaret , Cunningham Chris 
Published: June 2022

AbstractGiven high SARS-CoV-2 incidence, coupled with slow and inequitable vaccine roll-out in many settings, there is a need for evidence to underpin optimum vaccine deployment, aiming to maximise global population immunity. We evaluate whether a single vaccination in individuals who have already been infected with SARS-CoV-2 generates similar initial and subsequent antibody responses to two vaccinations in those without prior infection. We compared anti-spike IgG antibody responses after a single vaccination with ChAdOx1, BNT162b2, or mRNA-1273 SARS-CoV-2 vaccines in the COVID-19 Infection Survey in the UK general population. In 100,849 adults median (50 (IQR: 37–63) years) receiving at least one vaccination, 13,404 (13.3%) had serological/PCR evidence of prior infection. Prior infection significantly boosted antibody responses, producing higher peak levels and/or longer half-lives after one dose of all three vaccines than those without prior infection receiving one or two vaccinations. In those with prior infection, the median time above the positivity threshold was >1 year after the first vaccination. Single-dose vaccination targeted to those previously infected may provide at least as good protection to two-dose vaccination among those without previous infection.