bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic effects of the left atrial appendage exclusion (the heart hormone study)
Authors: Bartus Krzysztof , Elbey Mehmet A. , Kanuri Sri Harsha , Lee Randall , Litwinowicz Radoslaw , Natorska Joanna , Zabczyk Michal , Bartus Magdalena , Kapelak Boguslaw , Malecki Maciej T. , Lakkireddy Dhanunjaya 
Published: June 2022

AbstractIntroductionThe effect of epicardial left atrial appendage (LAA) occlusion therapy on lipid and glucose metabolism in atrial fibrillation (AF) patients over the long‐term follow‐up are unclear.MethodsIn a single‐center prospective observational study, 60 patients with longstanding persistent AF with cardiovascular risk factors had undergone an epicardial exclusion procedure. Anthropometric parameters and glucose, glycated hemoglobin (HbA1c), insulin, leptin, adiponectin, free fatty acids, beta‐hydroxybutyrate, and total cholesterol levels were evaluated on fasting at baseline before the procedure and compared with levels at 24 h, 7 days, 1, 3, 6, and 24 months follow the procedure.ResultsThe mean age of the patients was 67.5 ± 8.1. Insulin levels significantly increased at 7 days, 1, 3, 6, 12, and 24 months follow‐up. The leptin levels showed a significant increase in 6, 12, and 24 months when compared to baseline. Whereas the adiponectin levels showed a significant decrease at 3, 6, 12, and 24 months when compared to baseline levels. In patients with the epicardial procedure, when compared to baseline, glucose, glycated hemoglobin, total cholesterol, and beta‐hydroxybutyrate levels did not show any significant changes at baseline and 24 months follow‐up.ConclusionThe epicardial exclusion ligation in AF patients was associated with significant changes in insulin, leptin, and adiponectin over long follow‐up.
Pbrm1 intrinsically controls the development and effector differentiation of iNKT cells
Authors: Wang Xin , Lei Lei , Su Yanhong , Liu Jun , Yuan Ning , Gao Yang , Yang Xiaofeng , Sun Chenming , Ning Bin , Zhang Baojun 
Published: June 2022

AbstractUnder static condition, the pool size of peripheral invariant natural killer T (iNKT) cells is determined by their homeostatic proliferation, survival and thymic input. However, the underlying mechanism is not fully understood. In the present study, we found that the percentage and number of iNKT cells were significantly reduced in the spleen, but not in the thymus of mice with deletion of polybromo‐1 (Pbrm1) compared to wild type (WT) mice. Pbrm1 deletion did not affect iNKT cell proliferation and survival, instead significantly impaired their development from stage 1 to stage 2. Importantly, loss of Pbrm1 led to a dysfunction of RORγt expression and iNKT17 cell differentiation, but not iNKT1 and iNKT2 proportion. Collectively, our study reveals a novel mechanism of Pbrm1 controlling the peripheral size of iNKT cells through regulating their development and differentiation.
circRNA: Regulatory factors and potential therapeutic targets in inflammatory dermatoses
Authors: Liu Ruifeng , Zhang Luyao , Zhao Xincheng , Liu Jia , Chang Wenjuan , Zhou Ling , Zhang Kaiming 
Published: June 2022

AbstractThe skin is the largest organ of the human body and acts as the first line of defence against injury and infection. Skin diseases are among the most common health problems and are associated with a considerable burden that encompasses financial, physical and mental consequences for patients. Exploring the pathogenesis of skin diseases can provide insights into new treatment strategies. Inflammatory dermatoses account for a large proportion of dermatoses and have a great impact on the patients' body and quality of life. Therefore, it is important to study their pathogenesis and explore effective treatment. Circular RNAs (circRNAs) are a special type of RNA molecules that play important regulatory roles in several diseases and are involved in skin pathophysiological processes. This review summarizes the biogenesis, properties and functions of circRNAs as well as their roles in the pathogenesis of inflammatory dermatoses, including psoriasis, lupus erythematosus, atopic dermatitis, lichen planus and severe acne and their potential as therapeutic targets.
Immune responses to Vibrio vulnificus formalin‐killed vaccine and ghost vaccine in Scophthalmus maximus
Authors: Zhou Shun , Li Ying , Yi Jingyuan , Zheng Xujia , Huang Qing , Su Lin , Guo Baoshan , Yang Zongrui , Xiu Yunji 
Published: June 2022

AbstractIn this research, Vibrio vulnificus formalin‐killed (FKCs) vaccine and ghost (VVGs) vaccine were successfully developed, and shown to prevent vibriosis of Scophthalmus maximus resulting from V. vulnificus. The antibody titre of FKCs and VVGs vaccine was 1: 28 and 1: 211. The RPS of FKCs and VVGs vaccine was 60% and 80%. In order to improve the understanding of vaccine protection mechanism, transcriptome data was used to analyse the immune response of S. maximus infected with V. vulnificus after vaccination with FKCs and VVGs vaccine. In the SmCon and SmIV groups, a series of innate immune‐related genes were upregulated (such as, TLR5, Tp12, AP‐1 and IL‐1β) or downregulated (such as, CASP6 and CASP8), which suggested that the immune protection mechanism induced by inactivated vaccine was similar to that of autoimmune response. In the SmIV and SmGho group, a number of innate and adaptive immune‐related genes (such as, STAT1, IFN‐γ and MHC Ia) were activated, in which the expression of these genes was higher in SmGho, and VVGs vaccine induced stronger innate and acquired immune responses. In conclusion, the results lay a foundation for further study on the molecular mechanisms of immune protection induced by VVGs vaccine and FKCs vaccine.
Efficacy of capnographic and bispectral index monitoring on trans‐oral therapeutic endoscopy: A prospective observational study
Authors: Hayashi Yukie , Hosoe Naoki , Takabayashi Kaoru , Limpias Kamiya Kenji J. L. , Tojo Anna , Sakurai Hinako , Kinoshita Satoshi , Sujino Tomohisa , Nakayama Atsushi , Kato Motohiko , Yahagi Naohisa , Ogata Haruhiko , Kanai Takanori 
Published: June 2022

AbstractBackground and AimOral therapeutic and invasive endoscopy requires deep sedation to reduce patient distress due to prolonged examination and procedures. The usefulness of capnography and bispectral index (BIS) monitoring in the early hypoxia detection in oral therapeutic and invasive endoscopy has yet to be evaluated. This study aimed to investigate the clinical impact of capnography and BIS monitoring on hypoxic events during oral therapeutic and invasive endoscopic procedures.MethodsThis is a prospective observational study. Trans‐oral non‐intubated therapeutic and/or invasive endoscopic procedures were performed with conventional monitoring (pulse oximetry, pulse, and blood pressure) as well as additional monitoring (BIS and end‐tidal CO2 concentration). Hypoxia is defined as oxygen saturation of <90% that lasts >15 s. The clinical impact of capnography and BIS monitoring on hypoxic events during oral therapeutic and invasive endoscopic procedures were investigated with the risk factors for hypoxia in each patient.ResultsPatients with hypoxia had significantly more apneas detected using capnography than other patients. The multivariate analysis revealed the detected apnea by capnography as an independent risk factor for hypoxia (odds ratio: 3.48[95% confidence interval: 1.24–9.78], P = 0.02). The BIS was not significantly different as a risk factor for hypoxia; however, per‐event analysis revealed significantly decreased BIS values over time in 3 min before hypoxic events.ConclusionsApnea detected by capnography was an independent predictor of hypoxia. The BIS value was not associated with hypoxia events; however, it showed a significant downward trend before hypoxia events.
Clinicopathologic association and prognostic impact of microcystic, elongated and fragmented pattern invasion, combined with tumor budding in endometrioid endometrial cancer
Authors: Qi Xiqin , Zhu Lun , Zhang Bei 
Published: June 2022

AbstractAimAs a special invasive pattern seen in low‐grade endometrial carcinoma, microcystic, elongated and fragmented (MELF) pattern is related to lymph node metastasis. Tumor budding (TB) is another histological marker in many cancers associated with tumor aggressiveness. Herein, we evaluated the impact of MELF pattern combined with TB about clinicopathological features and prognosis in endometrioid endometrial cancer (EEC). To verify the relationship between the two morphological markers and microsatellite status in EEC, the primary mismatch repair (MMR) proteins were detected by immunohistochemistry.MethodsOne hundred and seventy‐two cases of ECC diagnosed between 2011 and 2016 were reviewed with a median follow up of 47.5 months. MELF pattern and TB were examined on all H&E‐stained slides. Primary MMR proteins (MLH1, MSH2, MSH6, and PMS2) were also detected.ResultsBased on MELF pattern and TB, 172 patients were divided into the following four groups: MELF(−)/TB(+) (n = 41), MELF(+)/TB(−) (n = 15), MELF(+)/TB(+) (n = 20), and MELF(−)/TB(−) (n = 96). Adverse pathological features were observed in the MELF(+)/TB(+) group: 70% presented deep muscular infiltration, 65% were lymphovascular space invasion, and 25% suffered lymph node metastasis. The proportion of MMR deficient in MELF(+)/TB(−) group was the highest (66.7%). The progression‐free survival (PFS) and overall survival (OS) among the four groups were significantly different. MELF(+)/TB(+) group showed the worst PFS and OS. As univariate and multivariate survival analyses revealed, the combination of MELF pattern and TB was confirmed as an independent predictor of poor prognosis.ConclusionsOur research demonstrates that MELF pattern combined with TB, as an independent predictor of adverse outcome, is associated with adverse pathological features, which facilitates better understanding of EEC tumor behavior and more precise prognosis without additional medical expense.
SPI1‐related protein inhibits cervical cancer cell progression and prevents macrophage cell migration
Authors: Jin Jing , Du Xin , Zhou Limin , Yao Dongmei , Zou Qian 
Published: June 2022

AbstractAimThe functions and molecular mechanisms of SPI1‐related protein (SPIB) were examined in cervical cancer (CC) cells.MethodsGenes related to miscarriage and prognosis in CC were identified by Kaplan–Meier and differential expression analysis, respectively. Cell proliferation, apoptosis, migration, and invasion were examined by cell counting kit‐8, flow cytometry, transwell migration, and transwell invasion assays, respectively. The potential functions and molecular mechanisms of SPIB in CC were speculated by gene set enrichment analysis (GSEA) analysis. The mRNA and protein levels of genes were examined by RT‐qPCR and western blot assays, respectively. The effect of SPIB on macrophage cells was tested by macrophage recruitment assay and bioinformatics analysis.ResultsA total of 753 dysregulated genes were identified in 88 TCGA CC samples with a history of one or more miscarriages versus 208 CC samples with no miscarriage history. Also, 91 genes related to CC prognosis were identified. SPIB, a gene related to both miscarriage and CC prognosis, inhibited Hela cell proliferation, migration, and invasion, and facilitated Hela cell apoptosis. GSEA analysis disclosed that SPIB might play vital roles in immunity, chemokine signaling pathway, and macrophage chemotaxis/activation in CC. Moreover, SPIB inhibited C‐X‐C motif chemokine ligand 8 (CXCL8), C‐C motif chemokine ligand 17 (CCL17), and C‐C motif chemokine ligand 25 (CCL25) expression in Hela cells, and SPIB overexpression in Hela cells hampered THP‐1 cell migration. Higher SPIB expression was associated with less M2 macrophage infiltration in CC.ConclusionsSPIB inhibited CC‐cell progression and hindered macrophage cell migration in CC.
Bur Choice When Removing Zirconia Restorations
Authors: Keeling Francis L. , Taft Robert M. , Haney Stephan J. 
Published: June 2022

AbstractPurposeRemoval of zirconia restorations can be challenging and time consuming, requiring dental burs with optimal cutting efficiency to minimize iatrogenic complications. Yet, there are very few burs marketed for this purpose and little evidence of their comparative benefit. This study evaluated one specialized bur and compared its cutting efficiency with three general‐purpose burs (one single use and two multiple uses).Materials and methodsThis study was designed to assess the cutting efficiency of 4 burs on a high‐strength zirconia substrate. One of these burs is a multiple‐use bur marketed for high‐strength ceramics (Komet 4ZR), another is a single‐use general‐purpose bur (Piranha 837.012 coarse), and two are multiple‐use general‐purpose burs (Brassler 6837.31.012C coarse and Komet 6837.314.012 coarse). The cutting efficiency of each bur was determined by measuring the time taken to perform serial 2 mm cuts into a block of zirconia. Ten burs in each group were tested with repeated cuts until the time taken for a 2 mm cut was twice that of the first 2 mm cut (50% loss of efficiency). This study was performed under reproducible conditions using a custom‐designed and fabricated rig assembly.ResultsThe results demonstrated a statistically significant difference in the cutting efficiency between bur types. The single‐use general‐purpose bur (Piranha 837.012) was the most efficient for all cut numbers p <2.2 × 10–16. The performance of all burs decreased with each cut, with cutting efficiency dependent on time point/cut number (p = 0.02). The rate of deterioration varied between burs, but this difference was not sufficient to be statistically significant.ConclusionsThis study demonstrated that a single‐use bur is more efficient than 3 multiple‐use burs. The cutting efficiency of a single‐use general‐purpose bur (Piranha 837.012 coarse) on Cerec Zirconia Mono L was statistically superior to that of multiple patient use burs in this study, including one marketed for use on high‐strength ceramics. Because single‐use burs are comparatively inexpensive and require no sterilization, they should be strongly considered for the removal of zirconia restorations.
Are early adopters willing to accept frozen, ready‐to‐cook mealworms as a food source?
Authors: Rovai Dominic , Amin Samir , Lesniauskas Ruta , Wilke Kristine , Garza Jeff , Lammert Amy 
Published: June 2022

AbstractLiterature suggests that edible insects are most likely to be accepted when they are presented in the nonvisible form, but is it the only way? This research examines the acceptability of whole, ready‐to‐cook mealworms among early adopters. An online questionnaire was developed and distributed without restriction via email, social media (Facebook) and in‐person referrals. Participants were assigned to predetermined clusters based on their willingness to adopt insects (n = 85 No‐thank‐you's, n = 85 Hideaways, n = 53 Daredevils, and n = 78 Peekaboo's), sent a follow‐up questionnaire which presented a video of frozen, ready‐to‐cook mealworms cooking, and asked their willingness to buy the product. Analysis of variance, Tukey's means comparisons, and principal component analysis maps were used to analyze the data. Early adopters (Daredevils–53% and Peekaboo's–38%), motivated by the novel experience and ease of preparation, would consume frozen, ready‐to‐cook mealworms in a stir‐fry, with vegetables, in a taco/burrito, or with rice.Practical ApplicationsThis work demonstrates the value of segmentation of consumers. By narrowing the target audience, this work suggests that frozen, ready‐to‐cook mealworms may be a viable product form for a niche segment of consumers. The adoption of mealworms in this form may be beneficial for entomophagy as a whole by increasing exposure and familiarity of insects as a food source. Potential benefits of this research include future product development and consumer evaluation opportunities to help better understand how to develop a product that consumers are willing to purchase in use as a substitute for traditional livestock proteins. Incremental shifts to purchasing edible insects in place of traditional livestock protein will have a positive sustainable impact on the food supply.
The Sleep Revolution project: the concept and objectives
Authors: Arnardottir Erna S. , Islind Anna Sigridur , Óskarsdóttir María , Ólafsdóttir Kristín A. , August Elias , Jónasdóttir Lára , Hrubos‐Strøm Harald , Saavedra Jose M. , Grote Ludger , Hedner Jan , Höskuldsson Sveinbjörn , Ágústsson Jón Skírnir , Jóhannsdóttir Kamilla Rún , McNicholas Walter T. , Pevernagie Dirk , Sund Reijo , Töyräs Juha , Leppänen Timo ,   Sleep Revolution
Published: June 2022

SummaryObstructive sleep apnea is linked to severe health consequences such as hypertension, daytime sleepiness, and cardiovascular disease. Nearly a billion people are estimated to have obstructive sleep apnea with a substantial economic burden. However, the current diagnostic parameter of obstructive sleep apnea, the apnea–hypopnea index, correlates poorly with related comorbidities and symptoms. Obstructive sleep apnea severity is measured by counting respiratory events, while other physiologically relevant consequences are ignored. Furthermore, as the clinical methods for analysing polysomnographic signals are outdated, laborious, and expensive, most patients with obstructive sleep apnea remain undiagnosed. Therefore, more personalised diagnostic approaches are urgently needed. The Sleep Revolution, funded by the European Union's Horizon 2020 Research and Innovation Programme, aims to tackle these shortcomings by developing machine learning tools to better estimate obstructive sleep apnea severity and phenotypes. This allows for improved personalised treatment options, including increased patient participation. Also, implementing these tools will alleviate the costs and increase the availability of sleep studies by decreasing manual scoring labour. Finally, the project aims to design a digital platform that functions as a bridge between researchers, patients, and clinicians, with an electronic sleep diary, objective cognitive tests, and questionnaires in a mobile application. These ambitious goals will be achieved through extensive collaboration between 39 centres, including expertise from sleep medicine, computer science, and industry and by utilising tens of thousands of retrospectively and prospectively collected sleep recordings. With the commitment of the European Sleep Research Society and Assembly of National Sleep Societies, the Sleep Revolution has the unique possibility to create new standardised guidelines for sleep medicine.
Past and recent farming degrades aquatic insect genetic diversity
Authors: Crossley Michael S. , Latimer Christopher E. , Kennedy Christina M. , Snyder William E. 
Published: June 2022

AbstractRecent declines in once‐common species are triggering concern that an environmental crisis point has been reached. Yet, the lack of long abundance time series data for most species can make it difficult to attribute these changes to anthropogenic causes, and to separate them from normal cycles. Genetic diversity, on the other hand, is sensitive to past and recent environmental changes, and reflects a measure of a populations' potential to adapt to future stressors. Here, we consider whether patterns of genetic diversity among aquatic insects can be linked to historical and recent patterns of land use change. We collated mitochondrial cytochrome c oxidase subunit I (COI) variation for >700 aquatic insect species across the United States, where patterns of agricultural expansion and intensification have been documented since the 1800s. We found that genetic diversity was lowest in regions where cropland was historically (pre‐1950) most extensive, suggesting a legacy of past environmental harm. Genetic diversity further declined where cropland has since expanded, even after accounting for climate and sampling effects. Notably though, genetic diversity also appeared to rebound where cropland has diminished. Our study suggests that genetic diversity at the community level can be a powerful tool to infer potential population declines and rebounds over longer time spans than is typically possible with ecological data. For the aquatic insects that we considered, patterns of land use many decades ago appear to have left long‐lasting damage to genetic diversity that could threaten evolutionary responses to rapid global change.
Rationally trust, but emotionally? The roles of cognitive and affective trust in laypeople's acceptance of AI for preventive care operations
Authors: Kyung Nakyung , Kwon Hyeokkoo Eric 
Published: June 2022

AbstractArtificial intelligence (AI) is transforming healthcare operations. Nevertheless, particularly in the context of preventive care, little is known about how laypeople perceive and accept AI and change their behavior accordingly. Grounded in a solid theoretical framework of trust, this study bridges this gap by exploring individuals’ acceptance of AI‐based preventive health interventions and following health behavior change, which is critical for preventive care providers’ operational and business performance. Through a randomized field experiment with 15,000 users of a mobile health app complemented by a survey, we first show that the use and disclosure of AI in preventive health interventions improve their effectiveness. However, individuals are less likely to accept and achieve the health behavior change suggested by AI than when they receive similar interventions from health experts. We also observe that the effectiveness of AI‐based interventions can be improved by combining them with human expert opinions, increasing their algorithmic transparency, or emphasizing their genuine care and warmth. These results collectively suggest that, different from conventional technologies, AI's deficient affective trust, rather than comparable cognitive trust, play a decisive role in the acceptance of AI‐based preventive health interventions. This study sheds light on the literature on the role of new‐age information technologies in behavioral operations management, consumer marketing, and healthcare as well as the role of trust in technology acceptance. Valuable practical implications for more effective management of AI for preventive care operations and promotion of consumers’ health behavior are also provided.
DNA fragmentation of equine cumulus cells from Cumulus–Oocyte complexes submitted to vitrification and its relationship to the developmental competence of the oocyte
Authors: Ortiz Isabel , Dorado Jesús , Pereira Blasa , Diaz‐Jimenez María , Consuegra César , Gosalvez Jaime , Hidalgo Manuel 
Published: June 2022

AbstractThe objectives of this study were to evaluate the effect of vitrification on the DNA fragmentation rate of equine cumulus cells and to assess its relationship to oocyte in vitro maturation (IVM) after vitrification. Cumulus cells (CC) from 14 mares were recovered from COCs, previously submitted to vitrification (VIT) and IVM. The DNA fragmentation rate of the cumulus cells (CC‐DF) was assessed using a chromatin dispersion test. CC‐DF rates between vitrified and control COCs were statistically compared by Student’s t‐test. The rates of CC‐DF from control COCs were lower than in vitrified COCs. The percentage of CC‐DF was not significantly different (p > .05) between groups of COCs able to reach metaphase II (MII > 0) and those in which oocyte maturation was not achieved (MII = 0). In conclusion, vitrification has a deleterious effect on the DNA fragmentation of equine cumulus cells; however, this parameter cannot be used as a predictor for IVM success after COCs vitrification.