bluuebunny/crossref_metadata_2025_split huggingface dataset:
MO732: Circulating Omentin-1 and Subclinical Atherosclerosis in Chronic Haemodialysis Patients: A Pilot Study
Authors: Presta Pierangela , Bolignano Davide , Coppolino Giuseppe , Crugliano Giuseppina , Arena Roberta , Ruosi Federico , Tripodi Loredana , Carullo Nazareno , Zicarelli Mariateresa , Comi Alessandro , Comi Nicolino , Cianfrone Paola , Andreucci Michele 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Chronic haemodialysis (HD) patients are notoriously at high risk for cardiovascular mortality and morbidity. Omentin-1 (OME-1) is an adipocytokine produced by adipose tissue, whose levels are altered in various dysmetabolic conditions, such as obesity and type 2 diabetes mellitus. Beyond this, nowadays there is evidence indicating that this cytokine plays a crucial role in the genesis and progression of systemic atherosclerosis since it may inhibit plaque formation. With this background in mind, we run a pilot investigation to evaluate the possible correlation between circulating OME-1 levels and mean intimal thickness (IMT) in a small, homogeneous cohort of patients treated by HD (‘HD patients’).

METHOD
From a source cohort of 45 HD patients, we selected 27 individuals suitable to be enrolled. These patients had a dialysis vintage &gt; 6 mo., were on a regular 4 h/3 times week HD regimen and had stable clinical conditions. OME-1 levels were measured in their blood by the ELISA together with common laboratory and clinical parameters before starting a mid-week HD session. All the patients then underwent carotid Doppler ultrasound for IMT measurement. A total of 18 healthy subjects were the controls.

RESULTS
In the whole cohort, mean IMT values were 0.75 ± 0.12 mm. However, 8/27 patients (29.6%) presented a pathological IMT (&gt;1 mm). Circulating OME-1 levels in the whole HD cohort were increased as compared with controls [763 (367–1423) versus 371 (228–868) ng/mL; P = 0.03]. However, HD patients with pathological IMT presented, on average, lower OME-1 levels as compared with others [483.25 (168.7–1743) versus 1155 (286–2324); P = 0.05]. In ROC analyses, OME-1 values ≤ 840 ng/mL held an 83.3 sensitivity and 66.7 specificity (AUC 0.716, 95% confidence interval 0.506–0.946) in identifying HD patients with pathological carotid thickening.

CONCLUSION
OME-1 may play a direct role in the atherosclerosis process also in HD patients. Further studies on larger and more heterogeneous HD cohorts are needed to confirm the usefulness of OME-1 plasma levels as potential biomarkers for diagnosing subclinical atherosclerosis and for stratifying the risk of atherosclerosis progression in high-CV risk in HD patients.
MO734: High Serum Oxalate Concentration Associated with Coronary Artery Calcification and Lanthanum Carbonate in Hemodialysis Patients
Authors: Nishizawa Yoko , Hirasawa Eriko , Tosaka Mai , Hosoda Yumi , Horimoto Ai , Omae Kiyotsugu , Nitta Kosaku , Ogawa Tetsuya 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Oxalate is an organic acid found in abundance in plants and can also be synthesized as a terminal metabolite by the organic acid metabolism systems in humans. As a major excretion pathway of oxalate is urine, serum oxalate (SOx) concentration elevates in end-stage renal disease (ESRD) patients and causes calcium oxalate deposition to tissues. We previously reported that calcium oxalate might be one of the major components of coronary artery calcification (CAC) in ESRD patients; however, the association between SOx and CAC is unknown. This study aimed to demonstrate the relationship between SOx and CAC in haemodialysis patients.

METHOD
A total of 77 patients undergoing maintenance haemodialysis at a single facility who underwent an atherosclerosis check-up from 2011 to 2012 were enrolled and measured SOx retrospectively in 2021. Of those, 17 extremely outlying SOx patients were excluded, and 60 patients including 42 males were analysed. The median age was 64 (35–85) years, and median dialysis duration was 87.5 (17.1–410) months. Gender, Agastston's CAC score, major artery calcification volume, laboratory data and medications around the atherosclerosis check-up date and new-onset cardiovascular disease (CVD) events and deaths during the 10-year observation period were recorded. A CVD event is defined as an admission due to non-fatal myocardial infarction, coronary artery disease or heart failure. SOx level was measured by the commercial colorimetric oxalate assay kit, and the normal SOx level was 181 µmol/L.

RESULTS
The median SOx was 267 (221–563) µmol/L and new-onset CVD events, all-cause deaths occurred in 28 (47%) and 22 (37%) patients, respectively. In univariate analysis, SOx was associated with males (r = 0.27, P = 0.03), serum albumin (r = –0.25, P = 0.05), uric acid (r = 0.29, P = 0.02), phosphate (r = 0.26, P = 0.04), alkaline phosphatase (r = –0.28, P = 0.03), lanthanum carbonate (r = 0.32, P = 0.01), major artery calcification volume (r = 0.28, P = 0.03) and CAC score (r = 0.29, P = 0.02). In multivariate regression analysis, SOx was associated with lanthanum carbonate (F = 5.96, P = 0.02) and CAC score (F = 4.47, P = 0.03, Table 1). Receiver operating characteristic curve showed SOx = 269.5 µmol/L was the best cut-off for predicting CVD events. We divided subjects into two groups by this cut-off SOx value and revealed SOx was associated with new-onset CVD events (N = 60, P = 0.01) even after adjusted by age under 75 years (N = 52, P = 0.03) or both age under 75 years and CAC score under 1000 (N = 28, P = 0.04) by the Kaplan–Meier method (Fig. 1).

CONCLUSION
SOx was associated with CAC score, lanthanum carbonate and CVD events in ESRD patients undergoing haemodialysis.
MO744: Evaluation of Epithelial to Mesenchymal Transition in Peritoneal Functional Decline and Cardiovascular Disease
Authors: Maria Arriero Pais Eva , Sandoval Pilar , González Guadalupe , Albar Patricia , del Peso Gilsanz Gloria , Auxiliadora Bajo Rubio María , Selgas Rafael , López-Cabrera Manuel 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Prior results demonstrated preliminary in peritoneal dialysis (PD) patients that mesothelial cells are able to undergo mesothelial to mesenchymal transition (MMT) by differential genomic reprogramming, correlating with local damage of the peritoneum and a final ultrafiltration failure [1].
Therefore, our main goal is to find biomarkers with prediction and diagnosis value of all PD-induced MMT changes and CVD outcomes in PD effluents, biopsies or plasma from a longitudinal retrospective study of a European patient cohort of CKD, PD and HD, correlating them with cardiovascular disease (CVD), systemic inflammation, patient outcomes and animal models along with the IMPROVE-PD consortium.

METHOD
Analysis of EU biobanks: interrelation of MMT and CVD markers by qRT-PCR, immunohistochemistry, 3D-transwell models, citometry (Magpix) and echocardiography.
Statistical analysis and machine learning (MA): correlograms, PCA modelling, K-means clustering, decision trees.

RESULTS

CONCLUSION
Despite the rising awareness of the need for the improvement of the PD-MMT process, patients still carry a heavy burden of CVD and low survival rates of 5 years. IMPROVE-PD intends to combat them from different clinical and research fronts.
MO746: Marinobufagenin, Left Ventricular Geometry and Cardiac Dysfunction in End-Stage Kidney Disease Patients
Authors: Patella Gemma , Presta Pierangela , De Rosa Salvatore , Greco Marta , Crugliano Giuseppina , Sabatino Jolanda , Cianfrone Paola , Andreucci Michele , Indolfi Ciro , Foti Daniela , Coppolino Giuseppe , Bolignano Davide 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Left ventricular hypertrophy (LVH) is remarkably prevalent among end-stage kidney disease (ESKD) on chronic dialysis and has a strong prognostic value for adverse outcomes. In experimental models, the endogenous cardiotonic steroid Marinobufagenin (MBG) promotes cardiac hypertrophy and accelerates uremic cardiomyopathy. In this study, we investigated the possible relationships between MBG, LV geometry and cardiac dysfunction in the clinical setting of ESKD.

METHOD
Plasmatic MBG was measured in 46 prevalent ESKD patients (n = 30 HD, n = 16 PD) together with a thorough laboratory, clinical, bioimpedance and echocardiography assessment. Different patterns of LV geometry were defined by left ventricular mass index (LVMi) and ventricular morphology. Diastolic dysfunction was diagnosed by the ASE/EACVI criteria.

RESULTS
MBG levels were significantly higher in ESKD patients than in healthy controls (0.89 ± 0.45 versus 0.59 ± 0.19 nnmol/L; P = 0.001) and more elevated in PD than in HD (1.09 ± 0.22 versus 0.78 ± 0.55 nnmol/L; P = 0.02). At univariate analysis, MBG was directly correlated with LVMi (R = 0.63; P &lt; 0.001), LAVi (R = 0.57; P &lt; 0.001), E/e’ (R = 0.57; P &lt; 0.001), RAVi (R = 0.42; P = 0.01) and NT-proBNP (R = 0.37; P = 0.01), whereas a significant inverse correlation was observed with extracellular volume at bioimpedance (R=–0.33; P = 0.02). At multivariate analyses, E/e’ (β=0.38; P = 0.009) and LVMi (β = 0.42; P = 0.02) remained the sole independent predictors of MBG. A statistically significant trend in MBG levels (p=0.01) was noticed across different patterns of LV geometry, with the highest values found in eccentric LVH (Fig. 1). MBG levels were higher in the presence of diastolic dysfunction (P = 0.01) and this substance displayed a remarkable diagnostic capacity in distinguish patients with normal LV geometry, LV hypertrophy and, particularly, eccentric LVH (AUC 0.888; P &lt; 0.0001) and diastolic dysfunction (AUC 0.79; p=0.001).

CONCLUSION
Deranged plasma MBG levels in ESKD patients on chronic dialysis may reflect alterations in LV structure and function. MBG may thus candidate as a novel biomarker for improving cardiac assessment in this high-risk population.
MO771: Haemodialysis Access Flow Measurement: Comparison of Ultrasound Dilution and Ultrafiltration Method on Nikisso DBB-EXA™ Dialysis Machine
Authors: Kosmadakis Georgios , Isnard Rouchon Myriam , Baudenon Julien , Deville Clemence , Enache Ioana , Gueret Claudine , Haskour Abraham , Necoara Aura , Rance Nicole 
Published: May 2022

Abstract

BACKGROUND AND AIMS
The regular measurement of the haemodialysis vascular access (VA) flow demonstrates a protective effect over thrombotic complications [1]. Transonic® Ultrasound dilution technique was first introduced in 1995 and progressively became a method of reference for recirculation and blood flow measurement on haemodialysis VA [2].
It is based on the technique of haemodilution with a certain volume of 0.9% NaCl and the measure of recirculation with inversed lines using the ultrasound technique. Consecutively the VA flow is estimated with a certain formula. Nikisso DBB-EXA™ dialysis machine has the capacity to measure the VA recirculation rate by photometry performing a controlled rise on ultrafiltration rate.
The aim of the present study is to compare the VA flow rate between the Transonic® Ultrasound dilution technique and the Nikisso DBB-EXA™ Ultrafiltration method.

METHOD
The primary outcome of the study is the association of VA flow measured by Transonic® and the Nikisso DBB-EXA™. During the first half of the same dialysis session, we measured the VA recirculation and flow rate twice with the two methods under a constant dialysis blood pump flow of 250–300 mL/min with the ultrafiltration and haemodiafiltration interrupted during the procedure after having reversed the dialysis lines.

RESULTS
The values are represented as mean ± SD. A total of 18 chronic haemodialysis patients (13 male: 5 female, mean age: 70 ± 14 years) participated in the study. They were haemodynamically stable without acute clinical conditions and were dialysed with a well-functioning arteriovenous fistula for at least 6 months. The mean value of recirculation with inverted dialysis lines for the Transonic® method was 20.79 ± 7.58% and for the Nikisso DBB-EXA™ was 24.43 ± 7.77. The mean values of VA blood flow with the Transonic® method were 1229 ± 588 mL/min and for the Nikisso DBB-EXA™ 1096 ± 490 mL/min. The measured values of the two methods presented a significant positive correlation (by Pearson) (R = 0.920, P &lt; 0.001) (Figure).

CONCLUSION
There is a significantly positive correlation concerning the VA flow measurement between the reference method of Transonic® and the Ultrafiltration method offered by the Nikisso DBB-EXA™. Larger studies are needed to confirm this correlation with an additional comparative analysis of the reproducibility of measurements.
MO772: Elevated C-Reactive Protein Level and Vascular Access Failure in End-Stage Renal Disease Patients: A Systematic Review and Meta-Analysis
Authors: Lai Yu , Fangning Wei , Liu Xusheng , Bao Kun , Qin Xindong 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Vascular access (VA) failure is common and can be fatal in hemodialysis (HD) patients with arteriovenous fistula (AVF) or arteriovenous graft (AVG). Most recent studies found that elevated C-reactive protein (CRP) level was associated with VA failure of AVF or AVG, although this is controversial, and have not been systematically reviewed.

METHOD
We systematically searched PubMed, Web of Science and Cochrane Library from inception until June 2021 for cohort and case-control studies on elevated CRP levels and VA failure in hemodialysis patients with AVF or AVG. We conducted a meta-analysis on the relative risk (RR) of VA failure and elevated CRP levels. An additional search of the Science Citation Index Expanded on the Web of Science and references lists were reviewed.

RESULTS
Of 2974 reports identified, 24 studies met inclusion criteria, most with high quality. We included 24 studies in the VA failure systematic review and 15 studies in the meta-analysis. A total of 12 cohort studies providing data for 4011 patients and 3 case-control studies providing data for 473 patients were reflected in the present meta-analysis. The risk of VA failure was higher [RR:1.14, 95% confidence interval (95% CI): 1.07–1.22] in the subjects with elevated CRP levels than in those with normal levels.

CONCLUSION
Elevated CRP level was associated with a higher risk of VA failure in hemodialysis patients with AVF/AVG.
MO777: The Impact of Morphological and Haemodynamic Characteristics of Blood Vessels on the Arteriovenous Maturation
Authors: Gjorgjievski Nikola , Dzekova-Vidimliski Pavlina , Rambabova- Bushljetik Irena , Dejanov Petar , Gerasimovska Vesna , Selim Gjulsen , Stojanoska Ana , Petronijevic Dzvezdana , Janevski Zoran , Severova- Andreevska Galina , Nikolov Igor , Spasovski Goce 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Doppler Ultrasound (DUS) is a superior tool for the assessment of arteriovenous fistula (AVF) functionality. The aim of the study was to determine the association between morphological and haemodynamic characteristics of the blood vessels with AVF maturation.

METHOD
The study was prospective that analysed 80 patients with chronic kidney disease (CKD) stage 4/5 and CKD stage 5 on haemodialysis (CKD5D) with newly created radio cephalic AVF. Haemodynamics and morphological evaluation of blood vessels by DUS was performed in all patients. The diameter of the cephalic vein, radial and brachial artery was measured: before creation, on the first day (24-h after creation) and at 4 weeks. The blood flow (BF) rate of AVF was calculated by DUS on day 1, at 4 and 8 weeks. The successful maturation of AVF was defined with BF ≥ 600 mL/min.

RESULTS
The mean age of the patients was 59.9 ± 13.5 years, of which 46 (57.50%) were males. Successful AVF maturation at 4 weeks was 43.75% and 62.5% at 8 weeks. The results of six adjusted models in multivariate analyses showed BF rate on day 1 was a predictor for AVF maturation at 4 (P &lt;0.001) and 8 weeks (P &lt;0.001). The receiver operating characteristic (ROC) analyses showed that the highest sum of sensitivity and specificity for the BF on day 1 was 395 mL/min (sensitivity 0.714 and specificity 0.889) for the outcome at 4 weeks; and 344 mL/min (sensitivity 0.860 and specificity 0.867) for the outcome measured at 8 weeks. The diameter of the blood vessel was AVF significantly larger in patients with mature AVF compared with patients with non-mature AVF. Male gender was significantly associated with AVF maturation of AVF at fourth week (P &lt; 0.001) and at eighth week (P = 0.001) compared with the female gender.

CONCLUSION
Our study showed that BF rate of AVF on day 1 was a predictor for AVF maturation at 4 and 8 weeks. The diameter of blood vessels and male gender were statistically associated with AVF maturation.
MO822: Humoral Response and Breakthrough Infections With SARS-COV-2 B.1.617.2 Variant in Vaccinated Maintenance Hemodialysis Patients
Authors: Cohen Hagai Keren , Wand Ori , Nacasch Naomi , Shashar Moshe , Grupper Ayelet , Erez Daniel , Shitrit Pnina , Benchetrit Sydney 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Breakthrough COVID-19 may occur in vaccinated people and may result from declining vaccine effectiveness or highly transmittable SARS-CoV-2 variants, such as the B.167.2 (delta) variant. The recent emergence of the Omicron (B.1.1.529) variant has heightened this issue.
We investigated risk factors and outcomes for infection with the delta variant among vaccinated hemodialysis patients.

METHOD
Patients on maintenance hemodialysis who received two doses of the BNT162b2 (Pfizer-BioNTech) vaccine were categorized into the study group who developed COVID-19 and controls who did not in retrospective, observational and comparative study.
We compared risk factors for developing COVID-19 between two study groups and assessed clinical outcomes, including 30-day mortality rates.

RESULTS
A total of 25 cases of breakthrough SARS-CoV-2 infection were compared with 91 controls without. Breakthrough infection was associated with chronic immunosuppressive treatment, hematological malignancies and low antibody levels against SARS-CoV-2 spike protein (P = 0.001, 0.006 and 0.4, respectively). All COVID-19 cases occurred at least 5-months after vaccination and were caused by the B.1.617.2 variant in at least 23/25 cases. COVID-19 was categorized as severe or critical disease in 11/25 patients (44%) and 52% required hospitalization and COVID-19-directed treatment. The source of infection was nosocomial in 6/25 cases (24%), and healthcare-related in additional 3/25 (12%). Mortality rate was 20%, and overall mortality was significantly higher in subjects who developed COVID-19 than in controls (odds ratio for all-cause mortality 7.3, 95% confidence interval 1.6–33.2; P = 0.004).

CONCLUSION
Breakthrough COVID-19 with the B.1.617.2 variant can occur in vaccinated hemodialysis patients and is associated with immunosuppression and a weaker vaccine immune response. Infections may be nosocomial and result in significant morbidity and mortality.
MO830: Evaluation of Physical and Emotional Symptoms in Chronic Hemodialysis Patients
Authors: Akagun Tulin , Kavraz Tomar Ozdem , Usta Murat , Baylan Süleyman 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Hemodialysis patients experience physical and psychosocial symptoms caused by comorbid illnesses and the psychosocial impacts of living with end-stage renal disease. The Dialysis Symptom Index (DSI) is the most frequently used instrument to assess symptoms of end-stage renal disease. The aim of this study was to evaluate the frequency and severity of symptoms experienced in patients on maintanence hemodialysis.

METHOD
Data were collected using a face-to-face interview method with a dialysis symptom index. Laboratory tests were recorded from the patients’ file.

RESULTS
A total of 97 patients from our dialysis unit were enrolled. Clinical findings and laboratory findings were shown in Figures 1 and 2. Prevalance and severity of symptoms were shown in Figure 3.

CONCLUSION
Physical and emotional symptoms are prevalent, can be severe, and are correlated directly with impaired quality of life and depression in maintenance hemodialysis patients.
MO845: COVID-19 Sequels in Nephrology CKD Patients More Prone to Fatality
Authors: Likaj Erjola , Shehaj Larisa 
Published: May 2022

Abstract

BACKGROUND AND AIMS
This strange, prolonged, very exhausting and costly pandemic still is remaining in our world showing us that we are so weak, so unprotected, so fragile. It initiated and continued mostly like a respiratory syndrome but soon after it was seen that it has a systemic impact and consequences in our body. There were patients presented with hematuria, proteinuria and deteriorating of kidney function after the infection. Direct kidney involvement, cytokine storm, coagulopathy, rhabdomyolysis, antibiotic use, sepsis and haemodynamic instability then acute tubular necrosis, all of these factors contribute in this picture of kidney failure. Very difficult to treat and to rehabilitate a part of these patients are going through kidney replacement therapy and a part are dying.

METHODS
We studied a population of 77 patients that had passed the COVID-19 infection, confirmed by RT PCR. Males 58% and females 42%. Mean age was 67.1 years old, the BMI 29.6. Comorbidities were present in our cohort in 59.7% only one comorbidity and in 31.1% of patients two comorbidities. Mean time from COVID-19 infection was 56 days. There were CKD 70% patients and 30% non-CKD. Diagnoses of CKD patients were: hypertensive nephrosclerosis 49%, diabetic nephropathy 32%, chronic glomerulonephritis 9%, ADPKD 8% and transplanted patients with CAN 1%.

RESULTS
It resulted that our patients had higher D dimer (mean 3660 mcg/dL = 7-fold higher), PCR (mean 35 mg/dL, 7-fold higher) and ferritin (mean 634 ng/dL). Levels of lymphocytes remained lower (mean 13.9%). Proteinuria was found in 92% and hematuria was found in 88% of our patients. There were a significant correlation between need for KRT and diabetic nephropathy was p = 0.037 and also between need for KRT and proteinuria presence was P = 0.048. It was seen that higher the BUN levels lower the lymphocytes counts (P = 0.028) and lower the time post COVID lower the albumin levels (P = 0.007). It was seen that patients with CKD were more prone to need for haemodialysis treatment during the hospitalization and the mortality rate was higher in this group of patients.

CONCLUSION
COVID-19 infection and its short term sequels play an important role on morbidity and mortality of CKD patients. It was a decisive factor for KRT in these patients. CKD patients with their immunosuppression, several drugs used, anemia, hypertension, diabetes are more prone for post COVID-19 complications, hospitalizations, dialysis and then death. Being aware of this dangerous infection and being vaccinated are the mainstay and crucial things for being healthy and being alive.