bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding the importance of quality control and quality assurance in preclinical PET/CT imaging
Authors: McDougald Wendy A. , Mannheim Julia G. 
Published: October 2022

AbstractThe fundamental principle of experimental design is to ensure efficiency and efficacy of the performed experiments. Therefore, it behoves the researcher to gain knowledge of the technological equipment to be used. This should include an understanding of the instrument quality control and assurance requirements to avoid inadequate or spurious results due to instrumentation bias whilst improving reproducibility. Here, the important role of preclinical positron emission tomography/computed tomography and the scanner's required quality control and assurance is presented along with the suggested guidelines for quality control and assurance. There are a multitude of factors impeding the continuity and reproducibility of preclinical research data within a single laboratory as well as across laboratories. A more robust experimental design incorporating validation or accreditation of the scanner performance can reduce inconsistencies. Moreover, the well-being and welfare of the laboratory animals being imaged is prime justification for refining experimental designs to include verification of instrumentation quality control and assurance. Suboptimal scanner performance is not consistent with the 3R principle (Replacement, Reduction, and Refinement) and potentially subjects animals to unnecessary harm. Thus, quality assurance and control should be of paramount interest to any scientist conducting animal studies. For this reason, through this work, we intend to raise the awareness of researchers using PET/CT regarding quality control/quality assurance (QC/QA) guidelines and instil the importance of confirming that these are routinely followed. We introduce a basic understanding of the PET/CT scanner, present the purpose of QC/QA as well as provide evidence of imaging data biases caused by lack of QC/QA. This is shown through a review of the literature, QC/QA accepted standard protocols and our research. We also want to encourage researchers to have discussions with the PET/CT facilities manager and/or technicians to develop the optimal designed PET/CT experiment for obtaining their scientific objective. Additionally, this work provides an easy gateway to multiple resources not only for PET/CT knowledge but for guidelines and assistance in preclinical experimental design to enhance scientific integrity of the data and ensure animal welfare.
Validation of a new scoring approach of a child dietary questionnaire for use in early childhood among low-income, Latino populations
Authors: Adams Laura E. , Sommer Evan C. , Truesdale Kimberly P. , Barkin Shari L. , Heerman William J. 
Published: October 2022

Abstract
Background
Measuring diet quality in early childhood requires time-intensive and costly measurements (e.g., 24-hour diet recall) that are especially burdensome for low-income, minority populations. This study aimed to validate a new method for calculating overall diet quality among low-income, Latino preschoolers.

Methods
This study was an observational study using data from a randomized controlled trial. Participants included parents of Latino preschoolers who reported child diet quality at baseline, 4-month, 7-month, 12-month, and 13-month follow-up. At each timepoint parents responded to a 28-item child dietary questionnaire (CDQ), based on the National Health and Nutrition Examination Survey (NHANES) dietary module, which generated the number of times/day that a child ate each of 28 foods in the past month. These 28 items were then used to create a total standardized child diet quality index (possible range 0-100), using a percent of maximum method. Parents were asked to complete three 24-hour diet recalls at the 13-month follow-up, from which the 2015 Healthy Eating Index (HEI) was derived. Construct validity was evaluated by Spearman’s rank correlations between the new child diet quality index and the 2015 HEI at the 13-month follow-up. Test-retest reliability was assessed by intraclass correlation coefficients (ICC) for sequential pairs of time points.

Results
Among 71 eligible parent-child pairs, mean child age was 4.2 (SD = 0.8) years, 50.7% of children were female, and mean child body mass index (BMI) was 17.8 (SD = 2.0) kg/m2. Mean Child Diet Quality Index was 45.2 (SD = 3.2) and mean HEI was 68.4 (SD = 10.5). Child Diet Quality Index and HEI total scores were significantly correlated (r = 0.37; p = 0.001). Test-retest ICCs were statistically significant between all sequential pairs of time points.

Conclusion
The new approach for calculating a measure of overall diet quality from the previously-validated 28-item dietary questionnaire demonstrated modest construct validity. When time and resources are limited, this new measure of overall diet quality may be an appropriate choice among low-income, Latino preschoolers.

Trial Registration
This reports presents observational data collected as a part of a clinical trial, which was registered on clinicaltrials.gov prior to participant enrollment (NCT03141151).
Absolute iodine concentration for dynamic perfusion imaging of the myocardium: improved detection of poststenotic ischaemic in a 3D-printed dynamic heart phantom
Authors: Hammel Johannes , Birnbacher Lorenz , Makowski Marcus R. , Pfeiffer Franz , Pfeiffer Daniela 
Published: October 2022

Abstract
Background
To investigate the detection capabilities of myocardial perfusion defects of dual-energy computed tomography (CT) technology using time-resolved iodine-based maps for functional assessment of coronary stenosis in a dynamic heart phantom.

Methods
An anatomical heart model was designed using a three-dimensional (3D) printing technique. The lumen of the right coronary artery was reduced to 25% of the original areal cross-section. Scans were acquired with a 64-slice dual-layer CT equipment using a perfusion protocol with 36 time points. For distinguishing haemodynamically affected from unaffected myocardial regions, conventional and spectral mean transit time (MTT) parameter maps were compared. A dose reduction technique was simulated by using a subset of time points of the time attenuation curves (TACs).

Results
The tracer kinetic modeling showed decreased errors on fit parameters from conventional to spectral TACs (42% reduction for A and 40% for λ). Three characteristic regions (highly, moderately, and not affected by the simulated stenosis) can be distinguished in all spectral perfusion maps. The best distinction was observed on MTT maps. An area under the curve (AUC) value of 1.00 for the voxel-wise differentiation of haemodynamically affected tissue was achieved versus a 0.89 AUC for conventional MTT maps. By temporal under-sampling, a dose reduction of approximately 78% from 19 to 4.3 mSv was achieved with a 0.96 AUC.

Conclusion
Dual-energy CT can provide time-resolved iodine density data, which enables the calculation of absolute quantitative perfusion maps with decreased fitting errors, improving the accuracy for poststenotic myocardial ischaemic detection in a 3D-printed heart phantom.
Fibromyalgia: epidemiology and risk factors, a population-based case-control study in Damascus, Syria
Authors: Alzabibi Mhd Amin , Shibani Mosa , Alsuliman Tamim , Ismail Hlma , alasaad Suja , Torbey André , Altorkmani Abdallah , Sawaf Bisher , Ayoub Rita , khalayli Naram , Kudsi Mayssoun 
Published: October 2022

Abstract
Background
Fibromyalgia is a chronic disease with a high burden. We aim to be the first to investigate the prevalence of fibromyalgia (FM) in Syria and assess its risk factors.

Methods
A self-reported questionnaire was distributed to the public to identify fibromyalgia patients using the American College of Rheumatology (ACR) 2010 modified criteria. Identified cases were matched using age with controls free from rheumatic disorders that were randomly sampled from the same population.

Results
Out of 2966 participants, 350 (11.8%) satisfied the diagnostic criteria. Of these, only 29 (8.2%) were previously diagnosed by a physician, 239 (68.3%) were females, and 69 (19.71%) were diagnosed with depression. Female sex (OR = 1.31), diagnosis of major depressive disorder (OR = 2.62), irritable bowel syndrome (OR = 1.8), and Restless legs syndrome (OR = 1.72) were associated with a higher likelihood of fibromyalgia.

Conclusion
Our study revealed one of the highest prevalence rates of fibromyalgia ever reported in the general population. Efforts must be intensified to increase awareness about this disease in Syrian society as well as among healthcare providers.
Functional rubber composites based on silica-silane reinforcement for green tire application: the state of the art
Authors: Neethirajan Jeevanandham , Parathodika Arshad Rahman , Hu Guo-Hua , Naskar Kinsuk 
Published: October 2022

AbstractSilica is the rubber industry’s most essential and cost-effective reinforcing filler after carbon black. The silica reinforcement mechanism with a non-polar elastomer is complicated by the presence of polar functional groups on the silica surface. This polar nature of silica causes filler-to-filler interaction by forming hydrogen bonds. Therefore, sizeable non-dispersed silica clusters remain in a non-polar rubber matrix. To avoid these strong filler-filler interactions and improve rubber/silica compatibility, the silica surface needs to be modified. This can be done using a coupling agent which has functional groups capable of linking both the rubber and silica. It has been discovered that when silica/silane coupling agents are present, the critical properties like rolling resistance and wet grip in the magic triangle of tire tread balance out better than carbon black formulations, bringing the system closer to the green tire goal. In this review article, the efforts made by both the rubber formulation development and chemistry to fully exploit the potential of silica/silane reinforcement for automotive tires are retrospected. Highlights on how compounding ingredients, process technology, functionalized elastomer, novel silanes, and the variant of silicas can enhance the magic triangle and silica-silane reaction mechanism are provided. In addition, the kinetics of silanization and measurements for the degree of silanization is also highlighted. Future research directions in this area are also touched upon. Hopefully, this review can stimulate future silica/silane scientific and technology developments for both academic and industrial-oriented requirements.
Surveillance and monitoring of early failing arteriovenous fistula using Doppler assessment in children on regular hemodialysis
Authors: Atwan Eman Fawzy , Awad Ahmad Sayed , Abdelrahman Safaa M. , Makar Samuel Helmy , Ghobashy Mohammed Hamadna Allah El 
Published: October 2022

Abstract
Background
A well-functioning vascular access is a mainstay to perform an efficient hemodialysis procedure. Limited lifespan of arteriovenous accesses is one of the major challenges of the current long-term hemodialysis therapy. Vascular access-related morbidity accounts for up to 50% of total dialysis patient’s costs. The pediatric vascular access clinical guidelines published by the National Kidney Foundation Kidney Disease Outcome Quality Initiative recommend establishing a surveillance strategy to detect access stenosis and direct patients for early intervention. The aim of this study was to test the role of using the color Doppler ultrasonography (CDU) as a surveillance method for arteriovenous fistulae in children on regular hemodialysis and its value for detecting the early vascular access failure or dysfunction to avoid access loss.

Results
During 10-month duration, we prospectively conducted surveillance for 30 patients on regular hemodialysis through arteriovenous fistulae (AVF) as a vascular access, clinical assessment of AVF and radiological examination using CDU of the upper limbs. Their ages were ranging from 2 to 18 years with 1:1 male: female ratio. Doppler indices were measured in the afferent arteries, at the site of anastomosis, and the draining veins. AVF stenosis was the highest among all the detected complications (n = 9, 30%), aneurysm and pseudoaneurysmal formation (n = 8, 26%) anastomoses diameter reduction in 16.6%, and it was correlated with the dialysis adequacy measured through the Kt/v. Other complications were thrombosis (n = 2, 6.7%), central venous stenosis (n = 2, 6.7%), steal syndrome (n = 1, 3.3%) and calcifications in (n = 1, 3.3%). In total, 26% of the patients have more than one malfunction. Only one case has access failure a month after CDU examination.

Conclusion
In conclusion, among pediatric patients CDU can detect early stenosis of AVF in children, which can affect the dialysis adequacy.
Spontaneous pregnancy rate after fallopian tube recanalization for unilateral obstruction with a patent contralateral tube
Authors: Fataftah Jehad , Tayyem Raed , Al Rshoud Firas , Al-Omari Mamoon 
Published: October 2022

AbstractBackgroundInfertility is a common sociomedical problem worldwide, affecting up to 15% of couples. Tubal obstruction is currently one of the most important causes of female infertility. This study was designed to determine the spontaneous pregnancy rate in patients undergoing unilateral transcervical fallopian tube recanalization, performed by an interventional radiologist, for proximal fallopian tube obstruction with a patent contralateral tube. The secondary objective was to analyze pregnancy rates in relation to the type and duration of infertility, patient age, and body mass index.ResultsThirty-eight patients with unilateral tube obstruction were included in this study. Transcervical fallopian tube recanalization was successfully performed in all cohorts. At the one-year follow-up; 13 women (34.2%) had become pregnant, nine (23.7%) delivered healthy babies, and four (10.5%) had miscarriages. The time interval between the application of the technique and spontaneous pregnancy was 1–12 months, with a mean of approximately 4 months. Multivariate analysis showed a significantly higher pregnancy rate in young (< 35 years), nonobese (BMI < 30 kg/m2) females with a history of primary infertility for < 5 years. Minor complications were noted in 27 patients (71%). No major complications were noted.ConclusionsOur study showed that transcervical fallopian tube recanalization of proximal fallopian tube obstruction with a patent contralateral tube increased the spontaneous pregnancy rate in cases of tubal factor infertility. Moreover, transcervical fallopian tube recanalization is recommended as a first-line treatment for women with unilateral proximal fallopian tube obstruction and a patent contralateral tube owing to the low risks associated.
Diagnostic utility of Bcl-2 immunohistochemical expression in pediatric functional bowel obstruction cases with ganglionated specimens
Authors: Mohamed Lobna Abd El Fattah , Hegazy Nedal Ahmed , Ghazal Faten Abd El Aziz , Zaki Ahmed Mohy El Din , Radwan Ahmed Bassiouny , Hakim Sarah Adel 
Published: October 2022

Abstract
Background
Functional disturbances of the gastrointestinal tract are caused by a number of neurodysplastic conditions, including diseases that are rarer than Hirschsprung’s disease (HSCR), such as ganglion cell immaturity and intestinal neuronal dysplasia (IND). Bcl-2 shows positive immunoreactivity in degenerative and immature ganglion cells (IGCs). This work evaluates the implication of the immunohistochemical expression of Bcl-2 in cases of pediatric intestinal pseudo-obstruction (IPO) with ganglionated specimens.
This 2-year prospective observational study was conducted from January 2017 to December 2019 on all intestinal specimens for cases referred from a pediatric surgery department with clinical data suggesting IPO (abdominal distension > 3 months, intermittent bilious vomiting, no radiological evidence of the transitional zone of HSCR or evidence of mechanical obstruction). The exclusion criteria were patients with IPO related to myopathic disorders, aganglionic biopsies, and specimens with inadequate tissue blocks. The same number of intestinal specimens of cases without IPO was used as a control group. All specimens underwent a histologic examination of ganglion cells and nerve bundles and were also evaluated for Bcl-2 immunohistochemical expression.

Results
Twenty-one specimens were analyzed, including six colonic resection specimens and 15 intestinal biopsies taken by full-thickness transanal biopsy (n = 12) or incisional biopsy from inadequately functioning stomas (n = 3). The mean ± standard deviation age of the patient cohort was 22 ± 7.4 months (range, 19–153 months), and there were 13 (61.9%) male patients. Bcl-2 protein was strongly positive in 57.1% of the cases, weakly positive in 19%, and negative in 23.8% as well as in all 21 control specimens. A highly significant correlation was observed between Bcl-2 expression and ganglion cell number (P < 0.001), where all hyperganglionic specimens showed strong positivity compared with the hypoganglionic and adequately ganglionic specimens. A positive association was also found between Bcl-2 expression and IND compared with other disorders of dysganglionosis (P = 0.04) and post-HSCR cases (P = 0.002).

Conclusion
Bcl-2 immunohistochemistry is a valuable tool to diagnose allied disorders of HSCR through its expression in IGCs, which are difficult to identify by conventional hematoxylin and eosin staining.
Informal Providers—Ground Realities in South Asian Association for Regional Cooperation Nations: Toward Better Cancer Primary Care: A Narrative Review
Authors: Nayak Prakash R. , Oswal Kunal , Pramesh Conjeevaram S. , Ranganathan Priya , Caduff Carlo , Sullivan Richard , Advani Shailesh , Kataria Ishu , Kalkonde Yogeshwar , Mohan Pavitra , Jain Yogesh , Purushotham Arnie 
Published: October 2022

PURPOSE South Asian Association for Regional Cooperation (SAARC) nations are a group of eight countries with low to medium Human Development Index values. They lack trained human resources in primary health care to achieve the WHO-stated goal of Universal Health Coverage. An unregulated service sector of informal health care providers (IPs) has been serving these underserved communities. The aim is to summarize the role of IPs in primary cancer care, compare quality with formal providers, quantify distribution in urban and rural settings, and present the socioeconomic milieu that sustains their existence. METHODS A narrative review of the published literature in English from January 2000 to December 2021 was performed using MeSH Terms Informal Health Care Provider/Informal Provider and Primary Health Care across databases such as Medline (PubMed), Google Scholar, and Cochrane database of systematic reviews, as well as World Bank, Center for Global Development, American Economic Review, Journal Storage, and Web of Science. In addition, citation lists from the primary articles, gray literature in English, and policy blogs were included. We present a descriptive overview of our findings as applicable to SAARC. RESULTS IPs across the rural landscape often comprise more than 75% of primary caregivers. They provide accessible and affordable, but often substandard quality of care. However, their network would be suitable for prompt cancer referrals. Care delivery and accountability correlate with prevalent standards of formal health care. CONCLUSION Acknowledgment and upskilling of IPs could be a cost-effective bridge toward universal health coverage and early cancer diagnosis in SAARC nations, whereas state capacity for training formal health care providers is ramped up simultaneously. This must be achieved without compromising investment in the critical resource of qualified doctors and allied health professionals who form the core of the rural public primary health care system.
Prognostic Value of FLT3-Internal Tandem Duplication Residual Disease in Acute Myeloid Leukemia
Authors: Grob Tim , Sanders Mathijs A. , Vonk Christian M. , Kavelaars Franҫois G. , Rijken Melissa , Hanekamp Diana W. , Gradowska Patrycja L. , Cloos Jacqueline , Fløisand Yngvar , van Marwijk Kooy Marinus , Manz Markus G. , Ossenkoppele Gert J. , Tick Lidwine W. , Havelange Violaine , Löwenberg Bob , Jongen-Lavrencic Mojca , Valk Peter J.M. 
Published: October 2022

PURPOSE The applicability of FLT3-internal tandem duplications ( FLT3-ITD) for assessing measurable residual disease (MRD) in acute myeloid leukemia (AML) in complete remission (CR) has been hampered by patient-specific duplications and potential instability of FLT3-ITD during relapse. Here, we comprehensively investigated the impact of next-generation sequencing (NGS)–based FLT3-ITD MRD detection on treatment outcome in a cohort of patients with newly diagnosed AML in relation to established prognostic factors at diagnosis and other MRD measurements, ie, mutant NPM1 and multiparameter flow cytometry. METHODS In 161 patients with de novo FLT3-ITD AML, NGS was performed at diagnosis and in CR after intensive remission induction treatment. FLT3-ITD MRD status was correlated with the cumulative incidence of relapse and overall survival (OS). RESULTS NGS-based FLT3-ITD MRD was present in 47 of 161 (29%) patients with AML. Presence of FLT3-ITD MRD was associated with increased risk of relapse (4-year cumulative incidence of relapse, 75% FLT3-ITD MRD v 33% no FLT3-ITD MRD; P < .001) and inferior OS (4-year OS, 31% FLT3-ITD MRD v 57% no FLT3-ITD MRD; P < .001). In multivariate analysis, detection of FLT3-ITD MRD in CR confers independent prognostic significance for relapse (hazard ratio, 3.55; P < .001) and OS (hazard ratio 2.51; P = .002). Strikingly, FLT3-ITD MRD exceeds the prognostic value of most generally accepted clinical and molecular prognostic factors, including the FLT3-ITD allelic ratio at diagnosis and MRD assessment by NGS-based mutant NPM1 detection or multiparameter flow cytometry. CONCLUSION NGS-based detection of FLT3-ITD MRD in CR identifies patients with AML with profound risk of relapse and death that outcompetes the significance of most established prognostic factors at diagnosis and during therapy, and furnishes support for FLT3-ITD as a clinically relevant biomarker for dynamic disease risk assessment in AML.
Sharing resources to advance translational research
Authors: Hooper Kirsty M. , Hmeljak Julija 
Published: October 2022

ABSTRACT
The publication of Resource articles is essential for the dissemination of novel, or substantially enhanced, tools, techniques, disease models, datasets and resources. By sharing knowledge and resources in a globally accessible manner, we can support human disease research to accelerate the translation of fundamental discoveries to effective treatments or diagnostics for diverse patient populations. To promote and encourage excellence in Resource articles, Disease Models & Mechanisms (DMM) is launching a new ‘Outstanding Resource Paper Prize’. To celebrate this, we highlight recent outstanding DMM Resource articles that have the ultimate goal of benefitting of human health.