bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ozone control as a novel method to improve health-promoting bioactive compounds in red leaf lettuce (Lactuca sativa L.)
Authors: Lee Jin-Hui , Goto Eiji 
Published: December 2022

In this study, we determined the short-term effects of ozone exposure on the growth and accumulation of bioactive compounds in red lettuce leaves grown in a controlled environment plant factory with artificial light, also known as a vertical farm. During cultivation, twenty-day-old lettuce (Lactuca sativa L. var. Redfire) seedlings were exposed to 100 and 200 ppb of ozone concentrations for 72 h. To find out how plants react to ozone and light, complex treatments were done with light and ozone concentrations (100 ppb; 16 h and 200 ppb; 24 h). Ozone treatment with 100 ppb did not show any significant difference in shoot fresh weight compared to that of the control, but the plants exposed to the 200 ppb treatment showed a significant reduction in fresh weight by 1.3 fold compared to the control. The expression of most genes in lettuce plants exposed to 100 and 200 ppb of ozone increased rapidly after 0.5 h and showed a decreasing trend after reaching a peak. Even when exposed to a uniform ozone concentration, the pattern of accumulating bioactive compounds such as total phenolics, antioxidant capacity and total flavonoids varied based on leaf age. At a concentration of 200 ppb, a greater accumulation was found in the third (older) leaf than in the fourth leaf (younger). The anthocyanin of lettuce plants subjected to 100 and 200 ppb concentrations increased continuously for 48 h. Our results suggest that ozone control is a novel method that can effectively increase the accumulation of bioactive compounds in lettuce in a plant factory.
CRISPR/Cas9-induced modification of the conservative promoter region of VRN-A1 alters the heading time of hexaploid bread wheat
Authors: Miroshnichenko Dmitry , Timerbaev Vadim , Klementyeva Anna , Pushin Alexander , Sidorova Tatiana , Litvinov Dmitry , Nazarova Lubov , Shulga Olga , Divashuk Mikhail , Karlov Gennady , Salina Elena , Dolgov Sergey 
Published: December 2022

In cereals, the vernalization-related gene network plays an important role in regulating the transition from the vegetative to the reproductive phase to ensure optimal reproduction in a temperate climate. In hexaploid bread wheat (Triticum aestivum L.), the spring growth habit is associated with the presence of at least one dominant locus of VERNALIZATION 1 gene (VRN-1), which usually differs from recessive alleles due to mutations in the regulatory sequences of the promoter or/and the first intron. VRN-1 gene is a key regulator of floral initiation; various combinations of dominant and recessive alleles, especially VRN-A1 homeologs, determine the differences in the timing of wheat heading/flowering. In the present study, we attempt to expand the types of VRN-A1 alleles using CRISPR/Cas9 targeted modification of the promoter sequence. Several mono- and biallelic changes were achieved within the 125-117 bp upstream sequence of the start codon of the recessive vrn-A1 gene in plants of semi-winter cv. ‘Chinese Spring’. New mutations stably inherited in subsequent progenies and transgene-free homozygous plants carrying novel VRN-A1 variants were generated. Minor changes in the promoter sequence, such as 1–4 nucleotide insertions/deletions, had no effect on the heading time of plants, whereas the CRISPR/Cas9-mediated 8 bp deletion between −125 and −117 bp of the vrn-A1 promoter shortened the time of head emergence by up to 2-3 days. Such a growth habit was consistently observed in homozygous mutant plants under nonvernalized cultivation using different long day regimes (16, 18, or 22 h), whereas the cold treatment (from two weeks and more) completely leveled the effect of the 8 bp deletion. Importantly, comparison with wild-type plants showed that the implemented alteration has no negative effects on main yield characteristics. Our results demonstrate the potential to manipulate the heading time of wheat through targeted editing of the VRN-A1 gene promoter sequence on an otherwise unchanged genetic background.
Perceived overall injustice and organizational deviance—Mediating effect of anger and moderating effect of moral disengagement
Authors: Qin Ge , Zhang Lihua 
Published: December 2022

IntroductionThis study is dedicated to exploring the influence of perceived overall injustice on employee anger and deviant behavior. Based on fairness heuristic theory and cognitive appraisal theory of emotion, a model was developed to investigate the relationship between perceived overall injustice, anger and organizational deviance. Based on social cognitive theory, the moderating role of moral disengagement was proposed.MethodsThe data were collected from three Chinese manufacturing corporations with a total effective sample size of 264. SPSS 26 and Mplus 8.3 were adopted to analyze data. Confirmatory factor analysis, descriptive statistics analysis and correlation results were illustrated. Hierarchical regression was used to test the model.ResultsStatistical results showed that there is a significant positive relationship among perceived overall injustice, anger and organizational deviance. The moderating effect of moral disengagement on the relationship between perceived overall injustice and organizational deviance is significant, while that on the relationship between anger and organizational deviance is insignificant.DiscussionThis study built a model to discuss the emotional and behavioral influences of perceived overall injustice. The findings suggest that individuals feel more anger as the level of perceived overall injustice increases, which thus lead to higher level of organizational deviance. Morally disengaged employees are more likely to engage in organizational deviance after being treated unfairly. However, the moderating effect of moral disengagement on the relationship of anger and organizational deviance was insignificant. The reason might be because anger is an aggressive emotion and individual experiencing anger may lead to impulsive behavior regardless of moral rules. Implications and limitations have been discussed.
Customer agility, market orientation, and brand image in the context of Chinese market
Authors: Junfeng Wang , Zesheng Yang , RuQiang Lai 
Published: December 2022

IntroductionCustomer agility (CA) and market orientation have been widely discussed in prior research. Also, the role of brand image in business making-decision has been emphasized. However, the current analysis lacks integrating the relationship among the three concepts. Thus, this study creatively creates a causal relationship model of CA, market orientation, and brand image.MethodsFinally, 289 valid samples from the managers in Chinese market was collected for further analysis by partial least squares structural equation modeling (PLS-SEM).ResultsThe results show that market orientation can positively affect CA (both customer-sensing capability and customer-responding capability), and CA will further have a positive influence on brand image. Also, market orientation has a direct impact on brand image. Additionally, CA (both customer-sensing capability and customer-responding capability) will mediate the impact of market orientation on brand image.DiscussionThe research has both theoretical and practical contributions. From the theoretical perspective, the results contribute to enriching the brand theory, strategy management, and marketing research. From the practical perspective, the investigation enlightens managers to pay attention to the roles of CA implementation and market orientation strategies in enhancing their brand image.
Body perceptions, occupations, eating attitudes, and behaviors emerged during the pandemic: An exploratory cluster analysis of eaters profiles
Authors: Monthuy-Blanc Johana , Corno Giulia , Bouchard Stéphane , St-Pierre Marie-Josée , Bourbeau Francisca , Mostefa-Kara Leïla , Therrien Émie , Rousseau Michel 
Published: December 2022

IntroductionCOVID-19 pandemic negatively impacted people’s mental and physical health. Three areas have been significantly impacted, among others: eating-related behaviors, occupational balance, and exposure to self-image due to videoconferencing. This study aims to explore and document eaters profiles that were reported during the pandemic in the general Canadian population using a holistic perspective, including body perceptions, attitudes, and eating behaviors (i.e., body image, behaviors, attitudes, and motivations regarding food), and occupations (i.e., physical activity and cooking).MethodsThis cross-sectional study was conducted from May to September 2020. Two hundred and seventy-three Canada’s residents, French speaking of 18 years of age and older, participated in an online survey on behaviors, attitudes, and motivations regarding food and eating as well as body image and occupations during the COVID-19 pandemic. A hierarchical cluster analysis was used to determine the eaters profiles. One-way ANOVA and Chi-square test were conducted to differentiate occupational characteristics between eaters profiles.ResultsThree distinctive profiles were found during the COVID-19 pandemic and could be placed on a continuum: the Congruent-driven eater is at the functional pole of the continuum, whereas the Incongruent-driven eater is at the dysfunctional pole of the eaters continuum. In the middle of the continuum, the Incongruent-perceptual eater is at a critical crossing point. Significant differences were reported between eaters profiles.DiscussionThe empirical results based on an eaters continuum conceptualization highlight the importance of understanding how people perceive their body to assess and promote food well-being.
Working toward a transdisciplinary approach to teaching and learning planetary health–A collective reflection
Authors: Dambre Cato , Strack Diaz Julia Gabriela , Orhan Rana , Montag Doreen , van der Zande Indira , Gallo Valentina 
Published: December 2022

BackgroundIn order to educate the next generation of leaders to work at reverting the damaging effects of the Anthropocene, there is an increasing need to incorporate more environmental-related aspects in all teaching programmes, including the health-related. Planetary health is a complex field which can benefit from a transdisciplinary pedagogical approach. The aim of this research was to evaluate an approach working toward transdisciplinarity applied to a course of Planetary Health taught at the Bachelor degree Global Responsibility &amp; Leadership of the University of Groningen through substantive feedback and reflections from the students.MethodsBy the end of the course, a focus group was conducted with the students inviting them to reflect on the different aspects of the pedagogical approach, evaluating their effectiveness. A thematic analysis was conducted on the transcribed focus group.ResultsThe students appreciated the added value of working toward a transdisciplinary approach and peer-to-peer learning and teaching adopted in the Planetary Health course, as a way of enhancing their learning experience. They pointed out the need of incorporating a transcultural approach into the transdisciplinary one, as a way not only to improve their learning experience, but also to enrich the transdisciplinarity itself.ConclusionIncorporating a process toward transdisciplinary and transcultural teaching of planetary health into undergraduate programmes was found to be of added value. The peer-to-peer horizontal learning opportunities were seen as a way for taking advantage of the collaborative, informal teaching and community building serving the overall scope of the course.
Night shifts, insomnia, anxiety, and depression among Chinese nurses during the COVID-19 pandemic remission period: A network approach
Authors: Peng Pu , Liang Mining , Wang Qian , Lu Lulu , Wu Qiuxia , Chen Qiongni 
Published: December 2022

BackgroundThe outbreak of the COVID-19 pandemic imposed a heavy workload on nurses with more frequent night shifts, which led to higher levels of insomnia, depression, and anxiety among nurses. The study aimed to describe the symptom-symptom interaction of depression, anxiety, and insomnia among nurses and to evaluate the impact of night shifts on mental distress via a network model.MethodsWe recruited 4,188 nurses from six hospitals in December 2020. We used the Insomnia Severity Index, Patient Health Questionnaire-9, and Generalized Anxiety Disorder Scale-7 to assess insomnia, depression, and anxiety, respectively. We used the gaussian graphical model to estimate the network. Index expected influence and bridge expected influence was adapted to identify the central and bridge symptoms within the network. We assessed the impact of night shifts on mental distress and compared the network structure based on COVID-19 frontline experience.ResultsThe prevalence of depression, anxiety, and insomnia was 59, 46, and 55%, respectively. Nurses with night shifts were at a higher risk for the three mental disorders. “Sleep maintenance” was the central symptom. “Fatigue,” “Motor,” “Restlessness,” and “Feeling afraid” were bridge symptoms. Night shifts were strongly associated with sleep onset trouble. COVID-19 frontline experience did not affect the network structure.Conclusion“Sleep maintenance,” “Fatigue,” “Motor,” and “Restlessness” were important in maintaining the symptom network of anxiety, depression, and insomnia in nurses. Further interventions should prioritize these symptoms.
Accurate classification of lung nodules on CT images using the TransUnet
Authors: Wang Hongfeng , Zhu Hai , Ding Lihua 
Published: December 2022

BackgroundComputed tomography (CT) is an effective way to scan for lung cancer. The classification of lung nodules in CT screening is completely doctor dependent, which has drawbacks, including difficulty classifying tiny nodules, subjectivity, and high false-positive rates. In recent years, deep convolutional neural networks, a deep learning technology, have been shown to be effective in medical imaging diagnosis. Herein, we propose a deep convolutional neural network technique (TransUnet) to automatically classify lung nodules accurately.MethodsTransUnet consists of three parts: the transformer, the Unet, and global average pooling (GAP). The transformer encodes discriminative features via global self-attention modeling on CT image patches. The Unet, which collects context by constricting route, enables exact lunge nodule localization. The GAP categorizes CT images, assigning each sample a score. Python was employed to pre-process all CT images in the LIDI-IDRI, and the obtained 8,474 images (3,259 benign and 5,215 lung nodules) were used to evaluate the method's performance.ResultsThe accuracies of TransUnet in the training and testing sets were 87.90 and 84.62%. The sensitivity, specificity, and AUC of the proposed TransUnet on the testing dataset were 70.92, 93.17, and 0.862%, respectively (0.844–0.879). We also compared TransUnet to three well-known methods, which outperformed these methods.ConclusionThe experimental results on LIDI-IDRI demonstrated that the proposed TransUnet has a great performance in classifying lung nodules and has a great potential application in diagnosing lung cancer.
Impact of main residential locations on depressive symptoms among older adults in China: A Blinder–Oaxaca decomposition analysis
Authors: Cheng Yuqing , Yu Qiutong , Li Wei , Zuo Genyong 
Published: December 2022

BackgroundWith the development of urbanization in China, the scale of internal migration and the number of immigrants among older adults are increasing. This requires paying attention to the living conditions and environment of immigrants. Many studies note a gap in the prevalence of depressive symptoms among older adults living in different main residential locations. However, few studies have examined the extent to which main residential locations influence depressive symptoms among older adults. This study aims to quantify the effect of main residential locations on depressive symptoms.MethodsFor this study, we used data from the 2018 Chinese Longitudinal Health and Longevity Survey and randomly selected 8,210 individuals aged 65 years and older were from the community to determine the effect of main residential locations on depressive symptoms among older adults. We further used the Blinder–Oaxaca decomposition method to quantify the explanatory factors of depressive symptom gaps among older adults and to estimate the relative effect of individual characteristics on depressive symptoms.ResultsIn this study, we noted significant differences in depressive symptoms among older adults in different main residential locations. Rural–urban migrants had higher depressive symptom scores (7.164). According to the Blinder–Oaxaca decomposition analysis, the high proportion of the depressive symptom gap can be explained by years of education, income, and exercise among different main residential locations groups. In addition, in the main parts of the explained differences, the proportions of the limitation of activities of daily living (2.28, 0.46, and −52.11%) showed opposite effects, while their share in different main residential locations groups varied widely.ConclusionUrbanization has resulted in more rural people moving to urban areas in China; Rural–urban migrants have the highest prevalence of depressive symptoms, which needs attention. Thus, there is an urgent need to integrate the health insurance and pension policy for urban and rural residents. This study provides a basis for formulating health policies and promoting the mental health of older adults in China as well as in low- and middle-income countries.
The Legacy of the Pandemic Preparedness Regime: An Integrative Review
Authors: Bourrier Mathilde S. , Deml Michael J. 
Published: December 2022

Objectives: The global response to COVID-19 inherited a long history of preparedness features pertaining to various threats, including bioterrorism, (re)-emerging infectious diseases, and pandemics. We describe the evolution of pandemic preparedness frameworks, before and after the COVID-19 pandemic.Methods: We conducted an integrative literature review of publicly available documents, including grey and scientific literature, on pandemic preparedness frameworks. We relied on social science literature as a main source and used search keywords: pandemic preparedness, H1N1, COVID-19, “whole-of-society”/“whole-of-community.”Results: The H1N1 pandemic (2009–2010) tested pandemic preparedness frameworks. Lessons-learned reports concluded that the global H1N1 response were too strong and unnecessarily alarming. Such critiques, pandemic fatigue, and budgetary cuts post-2008 explain lack of preparedness for COVID-19. Critiques culminated in a shift towards a “whole-of-society” approach to health crises, although its uptake has not been ideal.Conclusion: Traditional preparedness regime limits arose again during the COVID-19 pandemic. The “whole-of-society” approach was not fully deployed in COVID-19 responses. A “whole-of-organizations” approach could be designed, ensuring that countries consider local organizations’ potential to partake in containing infectious disease and counter undesirable side-effects of non-pharmaceutical measures.
Flow around an Oscillating Cylinder at Low Reynolds Number with Forced Convection: Effect of Corner Radius and Reynolds Number
Authors: Sarout Yuvraj , Islam Md. , Fatt Yap , Janajreh Isam 
Published: December 2022

This numerical study investigated the flow-induced vibration (FIV) on non-heated and heated cylinders with different normalized corner radii (r*) at different Reynolds numbers (Re). Four different values of r* were considered (i.e., 0 (square cylinder), 0.5, 0.75, and 1.0 (circular cylinder)) at three different Re: 100, 150, and 200 within the laminar regime. The cylinder constrained in the axial direction and oscillated transversally was considered for a fixed nondimensional cylinder mass (m*) of 10 and a reduced velocity (Ur) of 4.92. The effect of r* and Re could be seen in the vibration modes of cylinders. The two-dimensional incompressible Navier–Stokes and energy equations were solved together with Newton’s Second Law governing the motion of the cylinder with the help of a computational solver. Four different modes were observed in this study: Mode-I characterized by exceptionally low amplitude; Mode-II characterized by fluctuating amplitude known as hysteresis (beating); Mode-III characterized by high amplitude due to synchronization or lock-in; and Mode-IV characterized by the monotonic oscillation of fixed amplitude. For r* = 1, synchronization phenomenon/lock-in was observed. For the heated cylinder cases, due to the change in the normalized corner radius, a notable change in nondimensional vibrational amplitude A/D and the average Nusselt number Nuavg was seen. It was observed that A/D was higher when lock-in occurred (at Re = 100 and r* = 1), leading to a rise in Nuavg by 47.9% compared to Re = 100 and r* = 0. Due to the change in r*, a shifting phenomenon was observed at Re = 150, r* = 0.75 and Re = 200, r* = 1. A major change in Nuavg was observed from the circular cylinder to square cylinder at different Re. The beating phenomenon was observed at Re = 100 for r* = 0.75, which was similar to that occurring at Re = 150 and r* = 0.5, and those at Re = 200 and r* = 0. Heat transfer and wake structure parameters were found to be dependent on r* and Re.
Geometrization for Energy Levels of Isotropic Hyperfine Hamiltonian Block and Related Central Spin Problems for an Arbitrarily Complex Set of Spin-1/2 Nuclei
Authors: Stass Dmitri V. 
Published: December 2022

Description of interacting spin systems relies on understanding the spectral properties of the corresponding spin Hamiltonians. However, the eigenvalue problems arising here lead to algebraic problems too complex to be analytically tractable. This is already the case for the simplest nontrivial (Kmax−1) block for an isotropic hyperfine Hamiltonian for a radical with spin-12 nuclei, where n nuclei produce an n-th order algebraic equation with n independent parameters. Systems described by such blocks are now physically realizable, e.g., as radicals or radical pairs with polarized nuclear spins, appear as closed subensembles in more general radical settings, and have numerous counterparts in related central spin problems. We provide a simple geometrization of energy levels in this case: given n spin-12 nuclei with arbitrary positive couplings ai, take an n-dimensional hyper-ellipsoid with semiaxes ai, stretch it by a factor of n+1 along the spatial diagonal (1, 1, …, 1), read off the semiaxes of thus produced new hyper-ellipsoid qi, augment the set {qi} with q0=0, and obtain the sought n+1 energies as Ek=−12qk2+14∑iai. This procedure provides a way of seeing things that can only be solved numerically, giving a useful tool to gain insights that complement the numeric simulations usually inevitable here, and shows an intriguing connection to discrete Fourier transform and spectral properties of standard graphs.
A New Cryptic Species of the Genus Mychonastes (Chlorophyceae, Sphaeropleales)
Authors: Martynenko Nikita , Gusev Evgeniy , Kapustin Dmitry , Kulikovskiy Maxim 
Published: December 2022

A new species of green coccoid algae, Mychonastes hindakii sp. nov., was isolated from the River Moscow (Russia, Moscow). The taxon is described using morphological and molecular methods. Mychonastes hindakii sp. nov. belongs to the group of species of the genus Mychonastes with spherical single cells joined with mucilaginous, irregularly shaped stalks. A comparison of ITS2 rDNA sequences and its secondary structures combined with the compensatory base changes approach confirms the separation between Mychonastes hindakii and other species of the genus. Mychonastes hindakii sp. nov. represents a cryptic species that can only be reliably identified using molecular data.