bluuebunny/crossref_metadata_2025_split huggingface dataset:
The immunosenescence and lipotoxicity in thyrotoxicosis mice
Authors: Feng Qin 
Published: January 2022

Abstract
COVID-19 is a worldwide outbreak now, and it is found to be age-related. Immunosenescence may be a predisposing and severe factor for COVID-19. Besides, many infectious diseases in clinic are age-related, and elderly patients have longer hospitalization and worse prognosis. Therefore, finding suitable aging models is of great significance for fighting aging related diseases and promoting the prognosis of elderly patients. In this study, the relationship between thyrotoxicosis and aging was investigated by routine detection and serum metabonomics in mice. The results of routine blood test and flow cytometry showed significant decrease in neutrophils, lymphocytes, CD4+/CD8+ and CD4+IFN-γ + lymphocytes in thyrotoxicosis mice. Biochemical examination combined with serum metabolomics analysis showed that serious disorder of lipid metabolism may be one of the causes of immunosenescence, including lower cholesterol levels, lower levels of VD and bile acids, high level of glucocorticoids, triglycerides, free fatty acids, Sphingolipids and decrease of Docosanoids, especially DPA. This study proves that thyrotoxicosis mice are an accelerated aging model. In present study, the main performance is immunosenescence, which may be due to lipotoxicity, suggesting that the immunosenescence state can be adjusted by improving lipotoxicity, whether anti thyroxine or not. However, there are other manifestations of thyroid toxicity mouse model simulating aging, such as organ aging, which need to continue to be studied by means of system biology to provide more comprehensive evidence.
Critical SWOT analysis of fresh fruit handling practices by traders in Uganda’s Capital City. A rapid cross-sectional study
Authors: Nakayiza Suzan , Walekhwa Abel Wilson , Tumwine Gabriel , Muleme James , Musewa Angella 
Published: January 2022

Abstract
Poor fruit handling practices causes physical damage to fruits and exposes them to pathogenic microbial contamination with Salmonella spp, E. coli and Vibrio spp. These contribute to food borne illnesses such as Salmonellosis, Shigellosis, Cholera, E. coli O157:H7 infection Campylobacteriosis among others. An estimated 14 percent of all diseases registered at health centers are food borne related in Uganda making this a public health concern. The economic burden of foodborne diseases was estimated at about 300 million United States Dollars in 2016. A rapid cross-sectional study was conducted using a SWOT framework to understand the fruit handling practices by traders in one of the markets in Uganda’s Capital City (Kampala). Our study showed that there existed some strengths such as Presence of an established market leadership and presence of organized registered and unregistered trade and social groups. Furthermore, there existed weaknesses that needed immediate attention such as (a) lack of clean water, (b) selling fruits on dirty market floor which exposes consumers to contaminated fruits, (c) disposal of spoilt fruits in open waste areas contaminates the soil, clogs drainage channels, creates a bad smell in the market and attracts rodents to feed on disposed fruits. We therefore recommend, establishing a ‘One Health’ market task force made up of traders, suppliers, farmers, consumers, local council, public health scientists and Kampala Capital City Authority (KCCA) that can work with Ministry of Local Governments and Ministry of Health to train traders to implement appropriate fruit handling practices as detailed in KCCA market laws. The One Health task force can work with Ministry of Science and Technology to design and develop tools such as insulated fruit crates, raised fruit stands, and closed waste bins that could be used by market traders to improve fruit hygiene practices. The One health market taskforce will be in position to link with private international organizations such WASH international to draft proposals to provision for free clean water for the market which could improve fruit hygiene and market sanitation.
Vesical Gossypiboma with Urethral Migration Causing Acute Urinary Retention: A Case Report and Literature Review
Authors: Gebreselassie Kaleab Habtemichael , Mummed Ferid Ousman , Gebrehiwot Fitsum Gebreegziabher , Adam Mubarek Bargicho , Adam Ramzi Yesuf , Issack Feysel Hassen 
Published: January 2022

Abstract
Introduction:

Vesical gossypiboma is a retained surgical sponge in the urinary bladder following abdominal or pelvic surgery. It is probably under-reported due to medico-legal issues. It usually poses a diagnostic challenge to clinicians. A high index of suspicion is essential to make a timely diagnosis.
Case Presentation:

We present a case of vesical gossypiboma in a 64-year-old male patient who presented with a history of persistent lower urinary tract symptoms (LUTS) and acute urinary retention following trans-vesical prostatectomy. The urinary retention was relieved by a suprapubic cystostomy. On cystoscopic examination, a large whitish spongiform structure in the urethra and bladder was revealed. A gentle attempt of cystoscopic removal was failed. At exploration, a retained surgical sponge was revealed and removed from the bladder. Postoperatively, the patient reported improvement of symptoms and was passing urine normally.
Clinical Discussion:

Vesical gossypiboma is relatively rare as very few isolated cases have been reported, and its migration through the urethra is even rarer. Patients with intravesical gossypiboma may manifest with either acute or chronic symptoms due to complications. Gossypiboma is an avoidable condition, and its occurrence can be prevented by thorough exploration of the surgical site and count of surgical materials at the end of the procedure.
Conclusion

Vesical gossypiboma should be considered as a differential diagnosis in patients with persistent LUTS following open bladder or prostate surgery. Open surgical removal is one of the feasible treatment options. But prevention is a preferable solution.
Identification of Multi-omics Biomarkers and Construction of the Novel Prognostic Model for Hepatocellular Carcinoma
Authors: Yan Xiaokai , Xiao Chiying , Yue Kunyan , Chen Min , Zhou Hang , Liu Xiao 
Published: January 2022

Abstract
Background: Change in the genome plays a crucial role in cancerogenesis and many biomarkers can be used as effective prognostic indicators in diverse tumors. Currently, although many studies have constructed some predictive models for hepatocellular carcinoma (HCC) based on molecular signatures, the performance of which is unsatisfactory. To fill this shortcoming, we hope to construct a novel and accurate prognostic model with multi-omics data to guide prognostic assessments of HCC. Methods: The TCGA training set was used to identify crucial biomarkers and construct single-omic prognostic models through difference analysis, univariate Cox, and LASSO/stepwise Cox analysis. Then the performances of single-omic models were evaluated and validated through survival analysis, Harrell’s concordance index (C-index), and receiver operating characteristic (ROC) curve, in the TCGA test set and external cohorts. Besides, a comprehensive model based on multi-omics data was constructed via multiple Cox analysis, and the performance of which was evaluated in the TCGA training set and TCGA test set. Results: We identified 16 key mRNAs, 20 key lncRNAs, 5 key miRNAs, 5 key CNV genes, and 7 key SNPs which were significantly associated with the prognosis of HCC, and constructed 5 single-omic models which showed relatively good performance in prognostic prediction with c-index ranged from 0.63 to 0.75 in the TCGA training set and test set. Besides, we validated the mRNA model and the SNP model in two independent external datasets respectively, and good discriminating abilities were observed through survival analysis (P < 0.05). Moreover, the multi-omics model based on mRNA, lncRNA, miRNA, CNV, and SNP information presented a quite strong predictive ability with c-index over 0.80 and all AUC values at 1,3,5-years more than 0.84.Conclusion: In this study, we identified many biomarkers that may help study underlying carcinogenesis mechanisms in HCC, and constructed five single-omic models and an integrated multi-omics model that may provide effective and reliable guides for prognosis assessment and treatment decision-making.
Children with Strabismus and Amblyopia Presented Abnormal Spontaneous Brain Activities Measured through Fractional Amplitude of Low-frequency Fluctuation (fALFF)
Authors: Shi Yi-Dan , Liu Li-Qi , Liang Rong-Bin , Ge Qian-Min , Li Qiu-Yu , Pan Yi-Cong , Shu Hui-Ye , Zhang Li-Juan , Shao Yi 
Published: January 2022

Abstract
Purpose: Based on fMRI technology, we explored whether children with strabismus and amblyopia (SA) showed significant change in fractional amplitude of low-frequency fluctuation (fALFF) values in specific brain regions compared with healthy controls, and whether this change could point to the clinical manifestations and pathogenesis of children with strabismus to a certain extent.Methods: We enrolled 23 children with SA and same number matched healthy control in the ophthalmology department of the First Affiliated Hospital of Nanchang University, and the whole brain was scanned by rs-fMRI. The fALFF value of each brain area was derived to examine whether there is a statistical difference in the two groups. Meanwhile, ROC curve was made in a view to evaluate whether this difference proves useful as a diagnostic index. Finally, analyze whether changes in the fALFF value of some specific brain regions are related to clinical manifestations.Results: report to HCs children with SA presented a decreased fALFF values in left temporal pole: the superior temporal gyrus, right middle temporal gyrus, right superior frontal gyrus, right supplementary motor area. Meanwhile, they also showed higher fALFF values in specific brain areas, which included left precentral gyrus, left inferior Parietal, left Precuneus.Conclusion: Children with SA showed abnormal fALFF values in different brain regions. Most of these regions were allocated to the visual formation pathway. The eye movement-related pathway or other visual-related pathways, suggesting the pathological mechanism of the patient.
LncRNA-KCNQ1OT1 Promotes the Odontoblastic Differentiation of Dental Pulp Stem Cells via Regulating miR-153-3p/RUNX2 Axis
Authors: Lu Xiaohui , Zhang Jiawen , Lu Yuanzhou , Xing Jing , Lian Min , Feng Guijuan , Huang Dan , Wang Chenfei , Shen Nimei , Feng Xingmei 
Published: January 2022

Abstract
Background and Objective: Long non-coding RNAs (LncRNAs) play a key role in the odontoblastic differentiation. This study aimed to explore the role of LncRNA-KCNQ1OT1 in the odontoblastic differentiation of human dental pulp stem cells (DPSCs) and its possible mechanism. Methods: The expression of LncRNA-KCNQ1OT1, miR-153-3p, RUNX2 in the odontoblastic differentiation was detected by qRT-PCR. Interaction between LncRNA-KCNQ1OT1 and miR-153-3p and interaction between miR-153-3p and RUNX2 were detected by dual-luciferase assay. The cell viability of DPSCs was detected by cell counting kit-8 (CCK-8), and the effect of LncRNA-KCNQ1OT1 and miR-153-3p on the odontoblastic differentiation of DPSCs was observed by alizarin red staining, alkaline phosphatase (ALP) activity assay and Western blot for RUNX2, DSPP, DMP-1. Results: During odontoblastic differentiation of DPSCs, the expression of LncRNA-KCNQ1OT1 increased, miR-153-3p expression decreased, and RUNX2 expression increased. Dual-luciferase assay showed that LncRNA-KCNQ1OT1 sponges miR-153-3p and miR-153-3p targets on RUNX2. After LncRNA-KCNQ1OT1 and miR-153-3p expressions of DPSCs were changed, the cell viability was not notably changed, but the odontoblastic differentiation was notably changed which was confirmed with alizarin red staining, ALP activity and Western blot for RUNX2, DSPP, DMP-1. Conclusion: LncRNA-KCNQ1OT1 promotes the odontoblastic differentiation of DPSCs via regulating miR-153-3p/RUNX2 axis, which may provide a therapeutic clue for odontogenesis.
SER PROFESSOR NA PERSPECTIVA INCLUSIVA: SABERES, FAZERES E COMPROMISSOS EM UMA TRÍADE FORMATIVA
Authors: Paz Cláudia Terra do Nascimento , Wisch Tasia Fernanda 
Published: January 2022

Este estudo reflexivo foi construído com o intuito de problematizar a docência no contexto da educação brasileira, tratando especificamente de professores que atuam na sala de aula regular e que se encontram preocupados em qualificar suas práticas e que, ao mesmo tempo, estão buscando possibilidades para a concretização de uma educação verdadeiramente inclusiva. Para tanto, propomos a refletir sobre os saberes, os fazeres e os compromissos dos professores que atuam na perspectiva inclusiva, trazendo à tona a importância da formação docente para a discussão. Fundamentadas pelos estudos de Tardif (2002), López (2016) e Arroyo (2013, 2014) nossas reflexões estão assentadas no conceito de professor inclusivo, o qual é por nós entendido como aquele professional de que assume como protagonista de sua ação docente e dos processos de aprendizagem de todos seus estudantes. Nessa direção, estruturamos nossas reflexões a partir de uma proposição de uma tríade formativa, a qual pressupõe a relação entre saberes, fazeres e compromissos, interligados, compondo a construção permanente do profissional inclusivo. Defende-se que cada elemento da tríade possui uma interdependência dos demais, da mesma forma que todos eles dependem da formação permanente e da experiência de cada professor, sendo esses processos individuais e coletivos ao mesmo tempo.
THE CAPITAL OF THE OTTOMAN EMPIRE IN EUROPE: BELGRADE ON THE FRONTIER (SERHAD) CITY (1521-1789)
Authors: ÖZKAN Selim Hilmi 
Published: January 2022

Belgrade remained under the rule of many states, including Rome, Serbia, Hungary, and Bulgaria, until the Ottoman period. Since the city had long been an important crossroads, it was invaded many times. The fact that it was on the route to connect the Middle and West Europe with Asia Minor also played a key role in the invasion of Belgrade. Along with being on the way of important roads, Belgrade serves as a door for Europe. Known as the key to Europe, Belgrade could be conquered in 1521 by the Ottoman Empire although it was besieged many times. In the following years, Belgrade became one of the most strategic cities of the Ottoman Empire in the Balkans. Further, the city in the position of Captaincy of Danube remained significant in terms of its military, strategical and economical aspects until it was out of the Ottoman Empire’s hands.
This study is concerned with the importance of the city to the Ottoman Empire coupled with its economic and military importance, primarily using Ottoman archival documents. Although considerable research has been devoted to this topic, rather less attention has been paid to the political importance of the Belgrade City to the Ottoman Empire in the Europe. To this end, the current study specifically attempts to examine this issue.
Effect-based water quality assessment of rivers receiving discharges from legacy mines by using acute and chronic bioassays with two cladoceran species
Authors: Mano H. , Iwasaki Y. , Shinohara N. 
Published: January 2022

Abstract
Information about the ecotoxicological impacts of surface waters that receive discharges from legacy mines is valuable to infer the ecological impacts on natural environment for managing mine discharges. In this study, we investigated behavioural and reproductive responses of two cladoceran species Ceriodaphnia dubia and Daphnia magna to water samples collected from metal-contaminated and reference rivers near legacy mines in Japan. The toxicity identification evaluation (TIE) of water samples that caused D. magna immobility was conducted to evaluate the key metals causing acute toxicity. The results of our water quality assessment performed using two cladoceran species demonstrated modest to significant adverse effects on their behaviour and reproduction, suggesting the potential for ecotoxicological impacts on natural populations and communities at several contaminated sites that received mine drainage. The results of TIE of water samples that caused D. magna immobility indicated likely contributions of Zn and Cu. These results imply that effect-based water quality assessments such as ours can provide direct and unique evidence of the ecotoxicological impacts of metals in river waters, which will be useful for better understanding and predicting the ecological effects of these metals in the natural environment.
Desempenho escolar e diferentes papéis de participação no bullying: Um estudo transcultural
Authors: Zequinão Marcela Almeida , Medeiros Pâmella de , Silva Jorge Luiz da , Oliveira Lopes Luís , Pereira Beatriz Oliveira 
Published: January 2022

O objetivo deste estudo é comparar o desempenho escolar entre os diferentes papéis de participação no bullying em dois países (Brasil e Portugal). Consiste num estudo transcultural de corte transversal de cunho exploratório e amostragem intencional. 376 crianças e adolescentes (do 5º e 6º ano de escolas públicas e privadas) participaram deste estudo, sendo 197 de Portugal e 179 do Brasil. Os papéis de participação no bullying foram obtidos mediante a aplicação de uma escala sociométrica e o desempenho escolar foi avaliado pelas médias finais obtidas ao final do ano letivo de quatro disciplinas (ciências, inglês, matemática e português). Os dados foram analisados através da estatística descritiva e inferencial (teste Kruskal-Wallis e U de Mann-Whitney). Destaca-se que em Portugal as vítimas apresentaram melhor desempenho escolar. Já no Brasil não foram encontradas diferenças significativas. Os dados apontam que o desempenho escolar parece ser uma variável mais importante no estabelecimento dos papéis de participação no bullying em Portugal do que no Brasil, indicando que a relação entre essas variáveis não pode ser considerada característica padrão entre os envolvidos neste fenómeno em diferentes países.
Revisi taksonomi Bloom: Kognitif, afektif, dan psikomotorik
Authors: Nafiati Dewi Amaliah 
Published: January 2022

Revisi taksonomi Bloom menitikberatkan pada (1) perubahan aplikasi yang terdiri dari tiga bidang yaitu aplikasi bidang penyusunan kurikulum, aplikasi bidang instruksi pengajaran, aplikasi bidang assesment/ penilaian; dan (2) perubahan terminologi yang menekankan pada sub kategori sehingga penilaian menjadi lebih spesifik, mudah dalam menyusun penilaian pada kurikulum, serta mudah dalam menyusun instruksi pengajaran. Revisi taksonomi Bloom juga mengubah kata kunci operasional dari kata benda menjadi kata kerja dari level terendah sampai dengan level tertinggi.Terdapat perubahan yang sangat signifikan pada revisi taksonomi Bloom di domain kognitif yang terdiri dari dua dimensi yaitu dimensi pengetahuan dan dimensi proses kognitif. Dimensi pengetahuan berubah menjadi faktual, konseptual, prosedural, metakognisi. dimensi proses kognitif baru menjadi mengingat, memahami, mengaplikasikan, menganalisis, mengevaluasi, dan mencipta. Domain afektif meliputi rasa, nilai, apresiasi, antusiasme, motivasi, dan sikap tercermin pada perilaku/ attitude sehari-hari pada proses pembelajaran baik di dalam kelas maupun di luar kelas. Domain psikomotorik dirumuskan sebagai serangkaian kemampuan yang bersifat kongkrit dan abstrak. Bloom's taxonomic revision focuses on (1) application changes that consist of three fields, namely the application of curriculum development. application of teaching instruction area, application of assessment/ assessment field; and (2) changes in the terminology emphasizing sub-categories so that assessment becomes more specific, it is easy to arrange assessments in the curriculum, and easy to arrange instructions teaching. Revised Bloom's taxonomy also changed the operational keywords from words to verbs from the lowest level to the highest level. There is a very significant change in Bloom's revised taxonomy in the cognitive domain which consists of two dimensions, namely the dimension of knowledge and the dimension of cognitive processes. The dimension of knowledge changes into factual, conceptual, procedural metacognition. the dimension of the new cognitive process becomes remembering, understanding apply. analyze, evaluate and create. Affective domain includes taste, value, appreciation, enthusiasm, motivation. and attitudes are reflected in behavior everyday in the learning process both in the classroom and outside the classroom. Domain psychomotor formulated as a series of abilities that are concrete and abstract.
Using Health Concept Surveying to Elicit Usable Evidence: Case Studies of a Novel Evaluation Methodology
Authors: Mariakakis Alex , Karkar Ravi , Patel Shwetak N , Kientz Julie A , Fogarty James , Munson Sean A 
Published: January 2022

Background
Developers, designers, and researchers use rapid prototyping methods to project the adoption and acceptability of their health intervention technology (HIT) before the technology becomes mature enough to be deployed. Although these methods are useful for gathering feedback that advances the development of HITs, they rarely provide usable evidence that can contribute to our broader understanding of HITs.

Objective
In this research, we aim to develop and demonstrate a variation of vignette testing that supports developers and designers in evaluating early-stage HIT designs while generating usable evidence for the broader research community.

Methods
We proposed a method called health concept surveying for untangling the causal relationships that people develop around conceptual HITs. In health concept surveying, investigators gather reactions to design concepts through a scenario-based survey instrument. As the investigator manipulates characteristics related to their HIT, the survey instrument also measures proximal cognitive factors according to a health behavior change model to project how HIT design decisions may affect the adoption and acceptability of an HIT. Responses to the survey instrument were analyzed using path analysis to untangle the causal effects of these factors on the outcome variables.

Results
We demonstrated health concept surveying in 3 case studies of sensor-based health-screening apps. Our first study (N=54) showed that a wait time incentive could influence more people to go see a dermatologist after a positive test for skin cancer. Our second study (N=54), evaluating a similar application design, showed that although visual explanations of algorithmic decisions could increase participant trust in negative test results, the trust would not have been enough to affect people’s decision-making. Our third study (N=263) showed that people might prioritize test specificity or sensitivity depending on the nature of the medical condition.

Conclusions
Beyond the findings from our 3 case studies, our research uses the framing of the Health Belief Model to elicit and understand the intrinsic and extrinsic factors that may affect the adoption and acceptability of an HIT without having to build a working prototype. We have made our survey instrument publicly available so that others can leverage it for their own investigations.