bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of a lipid metabolism disorder model in ApoEb mutant zebrafish
Authors: Hu Yang-Xi , You Hong-Min , Zhu Rong-Fang , Liang Yu-Lai , Li Fang-Fang , Qin Yong-Wen , Zhao Xian-Xian , Liang Chun , Jing Qing 
Published: June 2022

Abstract
Background and aims: ApoEb is a zebrafish homologous to mammalian ApoE, whose deficiency would lead to lipid metabolism disorders (LMDs) like atherosclerosis. We attempted to knock out the zebrafish ApoEb, then establish a zebrafish model with LMD.Methods: ApoEb was knocked out using CRISPR/Cas9 system, and the accumulation of lipids were confirmed by Oil Red O staining, confocal imaging, and lipid measurements. The lipid-lowering effects of simvastatin (SIM), ezetimibe (EZE) and Xuezhikang (XZK), an extract derived from red yeast rice, were evaluated through in vivo imaging in zebrafish larvae.Results: In ApoEb mutant, significant vascular lipid deposition occurred, and lipid measurement performed in whole-body homogenate of larvae and adult plasma showed significantly increased lipid levels. SIM, EZE and XZK apparently relieved the hyperlipidemia in ApoEb mutants, and XZK had a significant inhibitory effect on the recruitment of neutrophils and macrophages.Conclusions: In this study, a LMD model has been established in ApoEb mutant zebrafish. We suggest that this versatile model could be applied in studying hypercholesterolemia and related vascular pathology in the context of early atherosclerosis as well as the physiological function of ApoE.
Scalable and CMOS compatible hardware authentication using silicon photonic physical unclonable functions
Authors: Tarik Farhan Bin , Famili Azadeh , Lao Yingjie , Ryckman Judson D. 
Published: June 2022

Abstract
We demonstrate the uniqueness, unclonability and secure authentication of N = 56 physical unclonable functions (PUFs) realized from silicon photonic moiré quasicrystal interferometers (QCIs). Compared to prior photonic-PUF demonstrations typically limited in scale to only a handful of unique devices and on the order of 10 false authentication attempts, this work examines > 103 inter-device comparisons and false authentication attempts. Device fabrication is divided across two separate fabrication facilities, allowing for cross-fab analysis and emulation of a malicious foundry with exact knowledge of the PUF photonic circuit design and process. Our analysis also compares cross-correlation based authentication to the traditional Hamming distance method and experimentally demonstrates an authentication error rate AER = 0%, false authentication rate FAR = 0%, and an estimated probability of cloning (POC) below 10− 30. This work validates the potential scalability of integrated photonic-PUFs which can attractively leverage mature wafer-scale manufacturing and automated contact-free optical probing. Such structures show promise for authenticating hardware in the untrusted supply chain or augmenting conventional electronic-PUFs to enhance system security.
Generation mechanism of surface micro-texture in axial ultrasonic vibration-assisted milling(AUVAM)
Authors: Zhang Zongbo , Liu Wengang , Chen Xintong , Zhang Yu , Xu Chunling , Wang Kai , Wang Wei , Jiang Xin 
Published: June 2022

Abstract
Although axial ultrasonic vibration-assisted milling (AUVAM) is promising for rapid and economical fabrication of 3D micro-texture on various surfaces, it has been a challenge to obtain micro-texture with small feature size and large height simultaneously. The mechanism and major influence factors for the formation of micro-texture by this method were explored in this study. It was found that the generation of micro-texture was mainly caused by cutting and extrusion from the flank face of the milling tool under ultrasonic vibration. Therefore, a novel 3D tool model that considered the relief angle, end cutting edge angle and the blade profile was established in simulation analysis. The good agreement between simulation and experimental results demonstrated that the influence of the flank face on the micro-texture was mainly attributed to the relief angle of the milling tool. It was the first time to report that both the height and the profile shape of the micro-texture unit were affected by the overlap and extrusion between the flank surface and the micro-texture. But this influence diminished with the increase of spindle speed. 3D sinusoid-shaped micro-texture with minimum width of 20 μm and height of 2 μm (fully reproduced the ultrasonic amplitude) was realized with the relief angle of 40o and spindle speed of 4000 rpm. Other typical textures of weave, shell, scale and corrugation were also presented to show the effective regulation of texture patterns in AUVAM. This work provides both theoretical and practical basis for such a low-cost, efficient and controllable 3D micro-texture preparation method.
Divergent subgenome evolution in the allotetraploid frog Xenopus calcaratus
Authors: Knytl Martin , Fornaini Nicola R. , Bergelová Barbora , Gvoždík Václav , Černohorská Halina , Kubíčková Svatava , Fokam Eric B. , Evans Ben J. , Krylov Vladimír 
Published: June 2022

Abstract
Allopolyploid genomes are divided into compartments called subgenomes that are derived from lower ploidy ancestors. In African clawed frogs of the subgenus Xenopus (genus Xenopus), allotetraploid species have two subgenomes (L and S) with morphologically distinct homoeologous chromosomes. In allotetraploid species of the sister subgenus Silurana, independently evolved subgenomes also exist, but their cytogenetics have not been investigated in detail. We used an allotetraploid species in Silurana—Xenopus calcaratus—to explore evolutionary dynamics of chromosome morphology and rearrangements. We find that the subgenomes of X. calcaratus have distinctive characteristics, with a more conserved a-subgenome resembling the closely related genome of the diploid species X. tropicalis, and a more rapidly evolving b-subgenome having more pronounced changes in chromosome structure, including diverged heterochromatic blocks, repetitive sequences, and deletion of a nucleolar secondary constriction. Basedon these cytogenetic differences, we propose a chromosome nomenclature for X. calcaratus that may apply to other allotetraploids in subgenus Silurana, depending on as yet unresolved details of theire volutionary origins. These findings highlight the potential for large-scale asymmetry in subgenome evolution following allopolyploidization.
Cystathionine γ-lyase mediates cell proliferation, migration, and invasion of nasopharyngeal carcinoma
Authors: Zhang Qian-Qian , Gao Ying-Ran , Zhang Yan-Xia , Jing Mi-Rong , Cai Chun-Bo , Wang Di , Qi Hui-Wen , Chen Hao-Jie , Li Tao , Li Yan-Zhang , Jiang Qi-Ying , Ji Xin-Ying , Wu Dongdong 
Published: June 2022

Abstract
Background
Nasopharyngeal carcinoma (NPC) is an epithelia-derived malignancy with a distinctive geographic distribution. Cystathionine γ-lyase (CSE) is involved in cancer development and progression. Nevertheless, the role of CSE in the growth of NPC is unknown.
Results
In this study, we found that CSE levels in human NPC cells were higher than those in normal nasopharyngeal cells. CSE overexpression enhanced the proliferative, migrative, and invasive abilities of NPC cells and CSE downregulation exerted reverse effects. Overexpression of CSE decreased the expressions of cytochrome C, cleaved caspase (cas)-3, cleaved cas-9, and cleaved poly-ADP-ribose polymerase, whereas CSE knockdown exhibited reverse effects. CSE overexpression decreased reactive oxygen species (ROS) levels and the expressions of phospho (p)-extracellular signal-regulated protein kinase 1/2, p-c-Jun N-terminal kinase, and p-p38, but promoted the expressions of p-phosphatidylinositol 3-kinase (PI3K), p-AKT, and p-mammalian target of rapamycin (mTOR), whereas CSE knockdown showed oppose effects. In addition, CSE overexpression promoted NPC xenograft tumor growth and CSE knockdown decreased tumor growth by modulating proliferation, angiogenesis, cell cycle, and apoptosis. Furthermore, DL-propargylglycine (an inhibitor of CSE) dose-dependently inhibited NPC cell growth via ROS-mediated mitogen-activated protein kinase (MAPK) and PI3K/AKT/mTOR pathways without significant toxicity.
Conclusions:
In conclusion, CSE could regulate the growth of NPC cells through ROS-mediated MAPK and PI3K/AKT/mTOR cascades. CSE might be a novel tumor marker for the diagnosis and prognosis of NPC. Novel donors/drugs that inhibit the expression/activity of CSE can be developed in the treatment of NPC.
Folic acid-Chitosan Coated Stylosin Nanostructured Lipid Carriers: Fabrication, In Vitro-In Vivo Assessment in Breast Malignant Cells
Authors: Sadeghi Soroush , Tabrizi Masoud Homayouni , Farhadi Amin 
Published: June 2022

Abstract
Objective: Synthesis of Targeted Nanostructure Lipid Carriers for stylosin (STY-CFN-NPs) delivery to MCF-7 CellsMaterials and Methods: STY-CFN-NPs were formulated via the homogenization and ultra-sonication technique. After evaluating the amount of drug encapsulation and FA binding, toxicity effect of the STY and STY-CFN-NPs on MCF-7 cells was measured by MTT method. Cell cycle analysis, AO/PI staining and qPCR to assess the inducing of apoptosis as well as Tubo cancer cell inoculated mouse model for antitumor properties of STY-CFN-NPs were used.Results: Significant increases in nanoparticle size and changes in zeta potential were observed after FA-CS coating on nanoparticles. Slow release of the STY within 144 h as well as acceptable rate for STY encapsulation efficiency (92.4% and FA binding (52.5%) to the STY-CFN-NPs (PS: 66.26±3.02 nm, ZP: 29.54±1.01 mV and PDI: 0.32±0.01) was reported. STY-CFN-NPs exhibited higher toxicity compared to STY suspension and treatment with STY-CFN-NPs was lead to the increased apoptotic cells, stopped cells in SubG1 phase and also increased Caspase and Bax expression and decreased BCL-2 and BCL-XL expression in In vitro and decreased the size of murine tumors (54.57% in 16 days) in In vivo.Conclusion: The results showed STY-CFN-NPs has good potential for breast cancer management
Nestin as a marker of unipotent epithelial progenitor cells differentiate into outer root sheath keratinocytes in embryonic and adult hair follicles
Authors: Baba Yuta , Onishi-Sakamoto Saki , Ide Kaori , Nishifuji Koji 
Published: June 2022

Abstract
Nestin is an intermediate filament protein transiently expressed in neural stem/progenitor cells. We previously demonstrated that outer root sheath (ORS) keratinocytes of adult hair follicles (HFs) in mice descend from nestin-expressing cells, despite being an epithelial cell lineage. This study aimed to define the exact stage when nestin-expressing ORS stem/precursor cells or their descendants appear during HF morphogenesis, and whether they are also recognized in adult HFs. Using Nes-Cre/CAG-CAT-EGFP mice, in which enhanced green fluorescent protein (EGFP) is expressed following Cre-based recombination driven by the nestin promoter, we found that EGFP+ cells appeared in the epithelial layer of embryonic HFs as early as the peg stage. EGFP+ cells in hair pegs were positive for keratin 5, but not vimentin, Sox2, Sox10, or S100 alpha 6. Tracing of tamoxifen-induced EGFP+ cells in postnatal Nes-CreERT2/CAG-CAT-EGFP mice revealed labeling of some isthmus HF epithelial cells in the first anagen stage. EGFP+ cells in adult HFs were not immunolabeled for keratin 15, an HF multipotent stem cell marker. However, when hairs were depilated to induce the anagen stage after tamoxifen injection, the majority of ORS keratinocytes in depilation-induced anagen HFs were labeled for EGFP. Our findings indicate that nestin-expressing unipotent progenitor cells capable of differentiating into ORS keratinocytes are present in both the HF primordia and adult HFs.
Hyperspectral image super-resolution reconstruction based on image partition and detail enhancement
Authors: Xu Yinghao , Lv Yuchao , Zhu Xijun , Liu Sifan , Sun Yuanyuan , Wang Yimin 
Published: June 2022

Abstract
The hyperspectral image (HSI) super-resolution (SR) reconstruction has attracted much attention and been used widely in various study fields due to its low requirements on hardware in practice. However, the distribution of image information is uneven. And HSI is treated equally during the process of super-resolution reconstruction. It is time consuming and many details cannot be extracted specifically. In this paper, a new method named MSDESR (multilevel streams and detail enhancement) is proposed to reconstruct HSI according to its uneven distribution of spatial information. The MSDESR consists of a submap shunt block, a high-low frequency information extraction with detail enhancement block, and a partition image reconstruction block. Firstly, the submap shunk block is designed to pre-classify hyperspectral images. The images are divided into complex and simple parts according to the spatial information distribution of the reconstructed submap. Secondly, the multiscale Retinex with detail enhancement algorithm is constructed to purify high-frequency noise-contaminated and enhance the image details by separating the samples into high and low frequency information. Finally, branching networks of different complexities are designed to reconstruct the images with high credibility and clear content. In this paper, datasets of QUST-1, Pavia University and Chikusei are applied in the experiments. The results show that, the MSDER outperforms state-of-the-art CNN-based methods in terms of quantitative metrics and visual quality, with quantities of 4.18% and 9.35% in the SRE and MPSNR metrics, respectively. Overall, the MSDER performs well in hyperspectral image super-resolution reconstruction, which is time saving and preserves the details of spatial information.
Multicentre study on stress coping strategies and vaginal/caesarean birth opinions among medical students
Authors: Michalik Anna , Zdun-Ryzewska Agata , Pracowity Michalina , Olszewska Jolanta 
Published: June 2022

Abstract
Background.
Polish perinatal care is facing a high and increasing caesarean birth (CB) rate, which is at 43%. Stress coping strategies among professionals can be important factors in the decision-making for CB. Study aimed to identify opinions associated with a birth route and stress coping strategies.
Methods.
This cross-sectional, exploratory study included 748 Polish medical students and aimed to identify opinions associated with a birth route and stress coping strategies among medical students. A descriptive questionnaire was distributed online. Group comparisons were performed using Welch’s t-test for continuous data or the χ2 test for categorical data. Spearman’s ρ coefficient was used.
Results.
Statistically, first- and second-year students thought that every woman can choose vaginal birth (VB) or CB. Students in their final years of study considered that VB is more beneficial for women at low risk of VB-related complications. The respondents who reported that VB is safer, women recover faster after it, and women should not be able to choose VB/CB on their own exhibited statistically significantly lower helplessness levels and greater use of religion as a stress coping strategy. Those who were more helpless and less religious were also less convinced of the safety of VB, believed in faster recovery after CB and expressed that the woman herself should decide how to give birth.
Conclusion.
Stress coping strategies seem to be related to the understanding of the childbirth process and the set of attitudes connected with it. Helplessness and religiosity turned out to be the most important factors in this area. The connection between helplessness (stress coping strategy) and estimation of greater pain during VB than CB obtained in our research, is a phenomenon that may lead students with these characteristics to prefer and recommend CB in their future practice.
Estimating the health burden of road traffic injuries in Malawi using an individual based model
Authors: Smith Robert Manning , Cambiano Valentina , Colbourn Tim , Collins Joseph H , Graham Matthew , Jewell Britta , Lin Ines Li , Mangal Tara D , Manthalu Gerald , Mfutso-Bengo Joseph , Mnjowe Emmanuel , Mohan Sakshi , Ng’ambi Wingston , Phillips Andrew N , Revill Paul , She Bingling , Sundet Mads , Tamuri Asif , Twea Pakwanja D , Hallet Timothy B 
Published: June 2022

Abstract
BackgroundRoad traffic injuries are a significant cause of death and disability globally. However, in some countries the exact health burden caused by road traffic injuries is unknown. In Malawi, there is no central reporting mechanism for road traffic injuries and so the exact extent of the health burden caused by road traffic injuries is hard to determine. A limited number of models predict the incidence of death due to road traffic injury in Malawi. These estimates vary greatly, owing to differences in assumptions and so the health burden caused on the population by road traffic injuries remains unclear.MethodsWe use an individual based model and combine an epidemiological model of road traffic injuries with a health seeking behaviour and health system model. We provide a detailed representation of road traffic injuries in Malawi, from the onset of the injury through to the final health outcome. We also investigate the effects of an assumption made by other models, that multiple injuries do not contribute to health burden caused by road accidents.Results Our model estimates an overall average incidence of death between 23.5 to 29.8 deaths per 100,000 person years due to road traffic injuries and an average of 180,000 to 225,000 disability-adjusted life years (DALYs) per year between 2010 and 2020 in an estimated average population size of 1,364,000 over the 10-year period. Our estimated incidence of death falls within the range of other estimates currently available for Malawi, whereas our estimated number of DALYs is greater than the only other estimate available for Malawi, the GBD estimate predicting and average of 126,200 DALYs per year over the same time period. Our estimates, which account for multiple injuries, predict a 22-58% increase in overall health burden compared to the model ran as a single injury model.ConclusionsRoad traffic injuries are difficult to model with conventional modelling methods, owing to the numerous types of injuries that occur. Using an individual based model framework, we can provide a detailed representation of road traffic injuries. Our results indicate a higher health burden caused by road traffic injuries than previously estimated.
Fibromyalgia and Sexuality: A Qualitative Study in Brazilian Women
Authors: NISIHARA RENATO , QUADROS MANUELA , SPRICIGO DHAYANE , CREMONEZ LARA , MUTTI GABRIELA , KRUKLIS SOFIA , DIAS REJANE , SKARE THELMA , NONE NONE , TWO NONE ,   NONE NONE3
Published: June 2022

Abstract

Sexuality is considered an integral part of being human and it is directly associated with quality of life. Any change among the factors that contribute to sexual function allow the sexual dysfuncton(SD) to occur. Fibromyalgia (FM) is a musculoskeletal rheumatic syndrome, manifested mainly by chronic generalized pain, fatigue and muscle stiffness, in which SD is highly prevalent. The association between these two entities is not completely understood, but may be related to FM symptoms and treatments. Despite the prevalence of SD in FM patients, little research on the subject is done and the topic is rarely addressed in clinical practice. We aimed to analyze the discourse of FM patients, emphasizing the personal experiences of each patient, in order to try to understand their perspectives about sexuality. This is a qualitative study, carried out through an online questionnaire, via the Google Forms platform, from 07/23/2020 to 08/21/2020. The answers were obtained from an open space for comments, which did not have a guiding question. The Content Analysis Method was used, that allowed the identification of 13 repetition patterns in the obtained answers. A total of 210 open responses were obtained. Among them, two categories were the most prevalent (1)- request for return on this research results or guidance and (2)- pain interfering with sexual function. We conclude that pain during sexual intercourse and education in the addressed topic were the leading topics identified in most responses.
Ce3+-induced fluorescence amplification of cooper nanoclusters based on aggregation-induced emission for specific sensing 2,6-pyridine dicarboxylic acid
Authors: Shen Jingxiang , Fan Zhefeng 
Published: June 2022

Abstract
A straightforward, cost-effective and biocompatible reduction approach was applied to fabricate soluble but non-luminous glutathione-stabilized copper nanocluster (GSH-CuNCs). Surprisingly, as high as 1×103 times fluorescence enhancement was acquired when Ce3+ was injected at an extremely low concentration of only 18 µM. Ce3+ outperformed other rare-earth metal ions in terms of inducing fluorescence amplification of the non-luminous GSH-CuNCs. Furthermore, Ce3+ was employed as a linker between GSH-CuNCs and target of 2,6-pyridine dicarboxylic acid (DPA), an excellent anthrax biomarker. As a result, the Ce3+/GSH-CuNCs ensemble was developed as a novel fluorescence probe based on aggregation-induced emission (AIE) mechanism to detect DPA in the "on-off" mode at a fast rapid. The fluorescence intensity of ultra-bright Ce3+/GSH-CuNCs was gradually quenched along with the progressive increase in DPA concentration, resulting from a stronger coordination ability between Ce3+ and DPA than Ce3+ and GSH, Ce3+ would fail to interact with GSH and detached from the surface of CuNCs. A good linear relationship from 0.3 µM to 18 µM was obtained under optimal conditions for DPA determination, with a limit of detection (LOD) of 0.19 µM. Additionally, its feasibility was successfully validated by testing actual water samples. This proposed method significantly promote the construction of ensemble type sensors in the form of metal-ion/metal nanoclusters based on AIE for measuring other analytes.
A Novel Responsive Sensor for Penicillium italicum Fruit Fungus Based on Mesoporous CaMn4O8-G-SiO2 Nanocomposite
Authors: Fatema Kamrun Nahar , Cho Kwang Youn , Jung Chong-Hun , Cho Ju Yong , Jang Won Kweon , Oh Won-Chun 
Published: June 2022

Abstract
While the mold that is blue-green Penicillium italicum is one of the most serious post-harvest plant infections, compromising the integrity of citrus and many other crops during storage and transportation, there is as yet no sensor for its detection on-site, or in the field. For the first time, we present the development of a novel CaMn4O8–G–SiO2 (CaMnGS) sensor to detect the presence of Penicillium italicum mold. CaMnGS was synthesized using a self-assembly technique in this study. CaMnGS sample demonstrated outstanding stability, high selectivity, and notable characteristics for Penicillium italicum fungus detection. For Penicillium italicum fungus sensing, the CaMnGS displayed a large linear range of (50–100) μL, and a low detection limit of 0.50 μL. Significantly, the CaMnGS sensor was capable of swiftly detecting the Penicillium italicum fungus in wastewater. The CaMnGS generated had no effect on cell survival and had tremendous potential for Penicillium italicum fungal sensing. The mesoporous CaMnGS sensor may also detect the presence or absence of the fungus Penicillium italicum. This method might be used to identify the fungus Penicillium italicum. This is also the first attempt to discuss the fabrication of an electrochemical sensor employing a mesoporous CaMnGS nanoparticle composite as a platform for the selective detection of Penicillium italicum.