bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paracandidimonas lacteus sp. nov., a urea-utilizing bacterium isolated from landfill
Authors: Zhang Chu , Pu Qian , Ying Junjie , Chen Ying , Li Chengze , Wu Hangtao , Wang Binjie , Zhang Yuhan , Zeng Guohong , Li Ou , Hu xiufang , Sun Cong 
Published: January 2022

Abstract
A urea-utilizing bacterium, designated Q2-2T, was isolated from landfill. Cells of strain Q2-2T were Gramstain-negative, aerobic, short-rod bacteria. Strain Q2-2T was observed to grow at a temperature range of 15-37℃ (optimum 30℃), a pH range of 5.5-9.5 (optimum pH 8.0) and 0-4% (w/v) NaCl (optimum 1%). The major respiratory quinone detected was Q-8, and the polar lipids identified were mainly diphosphatidyl glycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, and phosphatidyl glycerol. Based on the 16S rRNA gene sequence, strain Q2-2T had the highest similarity with Paracandidimonas caeni 24T (97.99%), Pusillimonas soli MJ07T (97.54%), Parapusillimonas granuli Ch07T (97.27%), Pusillimonas ginsengisoli DCY25T (97.09%) and Paracandidimonas soli IMT-305T (96.39%). The ANI values between strain Q2-2T and the above related type strains were 71.02%, 73.52%, 74.32%, 74.59% and 72.29%, respectively. The DNA G + C content of strain Q2-2T was 61.1%. Therefore, strain Q2-2T represents a novel species of the genus Paracandidimonas, for which the name Paracandidimonas lacteus sp. nov. (type strain Q2-2T= CGMCC 1.19179T =JCM 34906T) is proposed.
Association of Next Generation Sequencing and Routine Testing With Clinical Outcomes of Advanced Non-small Cell Lung Cancer Patients in a Real-world Setting
Authors: Song Zhengbo , Wang Wenxian , Si Jinfei , Zhang Xiaoyan , Ma Tonghui , Lou Guangyuan , Shi Zhiyong , Shao Lan , Zhang Yiping 
Published: January 2022

Abstract
Purpose Next generation sequencing (NGS) has been used frequently in advanced non-small cell lung cancer (NSCLC) patients. Here, we investigated the treatment selections and clinical benefits of NGS technologies for patients with advanced lung cancer in a real-world setting.Methods This retrospective study enrolled patients with advanced NSCLC who received genetic testing. Patients received either NGS or routine testing (EGFR and/or ALK and/or ROS1 only). Clinical outcomes were compared between patients received NGS and those undergoing routine testing. Propensity score analysis was used to prevent survival bias associated with patient characteristics. Overall survival (OS) was the primary outcome. Results Of 6,451 patients with advanced NSCLC, 5,666 (87.8%) patients underwent routine testing (RT) and 785 (12.2%) were tested by NGS. The median OS was 22.6 months and the 5-year survival rate was 22.89%. Comparison of OS showed no difference according to propensity score analysis (24.8 months vs 24 months, P=0.533). With uncommon mutations, propensity score analysis showed that 1 and 2-year survival rate was significantly higher in the NGS testing group than in the routine testing group. This could be due to the higher rate of patients receiving targeted therapy (68.0% vs. 53.6%; P=0.042) and off-label drugs (28.10% vs. 11.40%; P=0.002).Conclusion NGS was not independently with a significant benefit comparing with RT in most advanced NSCLC patients. NGS could provide clinical value for rare mutations that were not available in RT, and the future benefits of NGS in immunotherapy need to be further explored.
KCNQ1-deficient and KCNQ1-mutant human embryonic stem cell-derived cardiomyocytes for modeling QT prolongation
Authors: Song Yuanxiu , Guo Tianwei , Jiang Youxu , Zhu Min , Wang Hongyue , Lu Wenjing , Jiang Mengqi , Lan Feng , Cui Ming 
Published: January 2022

Abstract
BackgroundThe slowly activated delayed-rectifier potassium current (IKs) composed of KCNQ1 is one of the main currents in the process of repolarization. KCNQ1 mutation can lead to the occurrence of long-QT syndrome type 1 (LQT1). IKs does not participate in repolarization in mice; thus, no good model for the mechanism research and drug screening of LQT1 is currently available. In this study, we established a KCNQ1-deficient human cardiomyocyte (CM) model and performed a series of microelectrode array (MEA) detection using KCNQ1-mutant CMs constructed in other studies to explore the pathogenic mechanism of KCNQ1 deletion and mutation, and conduct drug screening.MethodKCNQ1 was knocked out in human embryonic stem cell (hESC) H9 line using the CRISPR/cas9 system. KCNQ1-deficient and KCNQ1-mutant hESCs were differentiated into CMs using a chemically defined differentiation protocol. Subsequently, high-throughput MEA analysis and drug intervention were performed to characterize the electrophysiological characteristics of KCNQ1-deficient and KCNQ1-mutant CMs.ResultsIn high-throughput MEA analysis, the electric field potential and action potential durations in KCNQ1-deficient CMs were significantly longer than those in wild-type CMs. KCNQ1-deficient CMs also showed an irregular rhythm. Furthermore, KCNQ1-deficient and KCNQ1-mutant CMs have different responses to different drug treatments, reflecting the differences in their pathogenic mechanisms.ConclusionWe established a human CM model with KCNQ1 deficiency, which showed prolonged QT interval and irregular heart rhythm. Simultaneously, we used various drugs to treat KCNQ1-deficient and KCNQ1-mutant CMs. The three models showed different responses to drugs. These models can be used as important tools for studying different pathogenic mechanisms of KCNQ1 mutation and the relationship between genotype and phenotype of KCNQ1, facilitating drug development.
Determinants of implanon discontinuation among women who ever used implanon in Shashamane district, West Arsi zone, Southern Ethiopia: Case-control study
Authors: Daba Sintayehu Gabisa , Gemechu Bikila Lencha , Ahmed Junayde Abdurahamen , Gedefa Lalisa Kebebe , Guda Henok Girma , Bankere Assefa Washo 
Published: January 2022

Abstract
Background: Despite tremendous work has been done on demand creation, capacity building and ensuring the logistics of Implanon; Implanon discontinuation rate remained high in Ethiopia. Risk factor of implanon discontinuation varies across different settings according to sociodemographic variations. However, there is a dearth of stronger evidences on those factors in the country and the study area in particular. Therefore, this study was designed to identify the determinants of Implanon discontinuation in Shashamane District, West Arsi Zone, Southern Ethiopia.Methods: A Community based unmatched case-control study was carried out in Shashamane district from April 12 to May 18, 2021 among 88 cases and 176 controls selected using systematic random sampling technique. A pretested, structured questionnaire with face to face interview was used. The collected data were entered using the EPI-INFO7.2.2 and analysed using Statistical Package for the Social Science (SPSS) version 25.0. Bi-variable and multivariable logistic regression analysis was performed and P-value of less than 0.05 was used to declare significance. Crude and adjusted odds ratio with respective confidence interval of 95% was used to show strength of association between variables.Result: The odds of Implanon discontinuation were more among women who can`t read and write [AOR:3.09(1.20- 8.00)], had < 4 living children [AOR:2.47(1.20-5.08)], no history of abortion [AOR:2.84(1.25-6.46)], not ever used contraceptive before Implanon [AOR:2.14(1.02-4.49)], counselled for <15 minutes [AOR:2.47(1.29-4.70)], had no discussion with her partner [AOR:2.88(1.42-5.84)], experiencing side effects after insertion [AOR:0.35(0.17-0.71)] as compared to their counterparts. Conclusion: This study found out that women educational status, number of living children, history of abortion, ever use of any contraceptive, duration of counselling, discussion with partner, and side effects were determinants of Implanon discontinuation. Thus, ensuring counselling before insertion of implanon, managing side effects would improve retention of implanon use. In addition, implanon discontinuation intervention should target partners, new acceptors and those with no formal education.
An Immune Related Gene Signature from Chemokines and Chemokine Receptors to Predict Tumor Prognosis and Immune Microenvironment Alteration in Lower-Grade Glioma (LGG) Based on Large-Scale Transcriptome Profile Analyses
Authors: Qi Chunxiao , Lei Lei , Li Ningning , Ou Shaowu 
Published: January 2022

Abstract
Chemokines and receptors are well known for their roles in mediating immune cell trafficking and lymphoid development; however, whether they can determine prognosis and immune microenvironment alterations in lower-grade glioma (LGG) remains uncertain. In this study, we acquired transcriptomic data from the Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA) and Gene Expression Omnibus (GEO) databases using a series of bioinformatics methods to estimate the prognostic value and potential biological function of candidate genes. We identified a four-gene (CCR4, CX3CL1, CXCL10 and CXCL11) signature to compose a risk score model. Kaplan-Meier curves and ROC curves verified the performance of risk score model in the TCGA, CGGA and GSE16011 datasets. The prognostic signature showed fair accuracy for 1-, 3- and 5-year overall survival (OS). The TIMER, TIP and CIBERSORT algorithms revealed different immune cell infiltration levels between the high- and low-risk score groups. LGG patients with high-risk scores had more malignant phenotypes and higher immune checkpoints expression as well as presented immunotherapeutic resistance. Therefore, we comprehensively analyzed the important role of chemokines and receptors in LGG patients; created a novel immune risk score model that could effectively reflect the prognosis and immune microenvironment alteration and presented a valuable indicator for immunotherapy in LGG patients.
Remote phosphor-based extended white light source pumped by single blue LED for efficient lighting
Authors: Dubey Atul Kumar , Trivedi Shivam , Kumar Vikas , Chanda Puspita , Mehta Dalip Singh 
Published: January 2022

Abstract
We report the development of an LED-based extended white light source containing a small cylindrical acrylic diffuser rod coated with YAG: Ce+3 phosphor and excited by a single 1W blue LED. This design supports remote phosphor coating technique and thermally isolates both LED as well as the phosphor layer. In this work, the blue light generated from GaN based LED chip was waveguided throughout the cylindrical diffuser and properly excited the phosphor layer for photoluminescence uniformity. The gradient diffusing technique was adopted for optimal light distribution of scattering and waveguiding of blue photons. The process dealt with both specular transmittance and bidirectional transmission distribution function (BTDF) of the diffuser so that the waveguided blue photons could propagate longer distance inside the diffuser rod via total internal reflection mechanism with simultaneous scattering due to gradient diffusing surface structure. The blue photons were found nearer to yellow phosphor behaved like an integrated chip-based system in remote phosphor structure. The scattering of blue photons was used in the photoluminescence process which was uniform throughout the diffuser length due to the selective diffusing technique. Because of this arrangement, the backward fluorescence and back-reflection of blue photons to the LED chip from phosphor layer were reduced significantly, resulting in the improvement of the source's luminance properties. The optical properties were properly analyzed, and it was found suitable for the use of general-purpose illumination-based applications.
Evaluation of the predictive Performance of Regional and Global Ground Motion Predictive Models against Greek Recorded Strong Motion Data.
Authors: Sotiriadis Dimitrios , Margaris Basil 
Published: January 2022

Abstract
The aim of the present work is to evaluate the Ground Motion Prediction Models (GMPMs), applicable in active shallow crustal regions of Greece, for peak response parameters (PGA, PGV) and various ordinates of acceleration spectra (5% damping), to be implemented in Probabilistic Seismic Hazard Analyses (PSHA). The evaluation is data-driven, taking into account the most updated strong-motion dataset for Greece. According to the authors’ knowledge and literature review, such an effort has not been attempted before for region of interest, as the selection of GMPMs for PSHA was made based on qualitative criteria. The steps which were followed to fulfill such a goal include: a) pre-selection of regional, pan-European and global GMPMs, suitable for implementation in Greece; b) selection of appropriate data-driven scoring methods; c) scoring of pre-selected GMPMs for both peak response intensity measures and spectral acceleration values; d) final selection of GMPM suites and weighting. The final selection of GMPMs describes adequately the epistemic uncertainty of strong-motion in Greece and provides a useful tool for addressing a major component of PSHA studies, that is GMPM selection.
Evaluation of Post-tracheostomy`s Scars and its Impact on Persons Quality of Life: A Case Control Study
Authors: Shahparonyan Rafik G. , Poghosyan Anna , Minasyan Armen M. , Bablumyan Aren Yu. , Malkhasyan Irina E. , Matosyan Khachatur H. , Sargsyan Ani M. , Tadevosyan Artashes E. , Muradyan Armen A. 
Published: January 2022

Abstract
Background: Tracheostomy is one of the more commonly performed procedures in critically ill patients. Postoperative scarring is one of the bothersome sequelae of tracheostomies. Scars distorted the physical appearance, especially when found on the head and neck, which could cause a negative impact on quality of life. The aim of this study was to evaluate and assess the impact on quality of life of post-tracheostomy`s scars, depending on the method of tracheostomy.Methods: The prospective, single-center observational case-control study was conducted. One hundred and fifty-six persons with more than four months of post-tracheostomy surgical scars presence were observed using the Patient and Observer Scar Assessment Scale and Dermatology Life Quality index questionnaire. Persons were divided on two groups depending on method of performed tracheostomy, and duration of cannulated period was considered in both groups. Statistical analyses were performed by using SPSS ver. 16.0 (SPSS Inc., Chicago, IL, USA) and p-values of <0.05 were considered significant.Results: The patients who had a tracheostomic tube cannulation period of less than 15 days had better cosmetic results than those who had tracheostomic tubes for more than 15 days, regardless of the method of tracheostomy 6.64± 0.082 vs. 16.15± 0.096 (p<0.001) in surgical tracheostomy group and 7.26± 0.211 vs.14.17± 0.379 (p<0.05) in percutaneous dilatational group. The Dermatology Life Quality index scores have shown a mean value of 0.6 ±0.01, which means that post-tracheostomy scarring in the presented study, had no effect on the person's quality of life.Conclusions: Aesthetic outcomes of post-tracheostomy`s scars after the open surgical tracheostomy technique, performed using a minimally invasive approach, did not statistically differ from those of the percutaneous dilatational technique. Persons with a long duration of tracheostomic tube ventilation showed worse aesthetic outcomes compared with short-term tracheostomic cannulation, not dependent on the tracheostomy performing technique.The Dermatology Life Quality index have shown that post-tracheostomy`s scarring in the presented study had no effect on the person's quality of life.Trial registration: ISRCTN24668317 (IRB no.YSMU №7/18-19). Date of registration 23/04/2019
Transient Visual Disturbance is Associated With Disability And Suicidal Risk In Patients With Migraine Without Aura
Authors: Tsao Yu- Chien , Wang Yen-Feng , Fuh Jong-Ling , Chen Wei-Ta , Lai Kuan-Lin , Liu Hung-Yu , Wang Shuu-Jiun , Chen Shih-Pin 
Published: January 2022

Abstract
Objectives: To investigate the characteristics and clinical correlates of transient visual disturbances (TVDs) in patients with migraine without aura (MO).Background: Patients with MO frequently report TVD other than typical visual aura, but the clinical significance of the TVDs is not determined.Method: Patients with MO that attended our headache clinics were enrolled. Headache profiles, disability, comorbidities, lifetime suicidal ideation and attempts were acquired with structured questionnaires. A semi-structured visual phenomenon questionnaire was also used to assess the characteristics of TVDs. Headache specialists interviewed with the participants for the ascertainment of diagnosis and verified the questionnaires.Result: MO patients (n = 7,200, female/male = 3.56, mean age: 40.1 ± 13.4 years) were divided into 2 subgroups based on the presence (n=2,488) or absence of TVDs (n= 4,712). Patients with TVD had higher headache-related disability, more psychiatric comorbidities and were more photophobic. Suicidal ideation and attempts were more common in patients with TVD than in those without (ideation: OR = 1.92 [95% CI: 1.71−2.15], p < 0.001; attempt: OR = 2.23 [95% CI: 1.80−2.75], p < 0.001).Conclusion: Presence of transient visual disturbance may suggest a higher migraine-related disability, higher level of photophobia and higher suicidal ideation/attempts in MO patients.
Teratogenic Potential of Solenstemma argel Extract in Wistar Albino Rats
Authors: Mustafa Nazik M.E. , Satti Shahenaz , Osman Nafisa A. , A.Gameel Ahmed , El-hadiyah Tarig M. 
Published: January 2022

Abstract
The majority of people in Africa receive their basic health care through herbal treatments. Herbal medicine may negatively impact fetal development irreparably. This study examined the teratogenic potential of Solenstemma argel extract in pregnant rats. Pregnant rats were treated with Solenstemma argel from 7th to 16th day of gestation. The dosage used was 250 mg/kg, intraperitoneal.Solenstemma argel extract treated group showed fetal abnormalities appeared as body hemorrhage, limbs abnormalities and resorption of fetuses. These appears in 25% of the fetuses (P-value = 0.01) which is significantly differed from control group. Furthermore, histopathological findings of liver sections from fetuses of Solenstemma argel - treated mothers showed loose liver texture and hepatocytes hemorrhage.In this study, we conclude that the use Solenstemma argel extract during the organogenesis period in pregnant rats has the potential to cause teratogenic effects, as well as abnormalities in liver histopathology.
Kooperation von Elternhaus und Schule zum Thema ‹digitale Medien›
Authors: Bonanati Sabrina , Kurock Ricarda , Gruchel Nicole , Buhl Heike M. 
Published: January 2022

Die Kooperation zwischen Elternhaus und Schule kann Eltern in ihren Fähigkeiten zur Internetnutzung und -gestaltung sowie in der Wahl passender Medienerziehungsstrategien ihrer Kinder unterstützen. Voraussetzung dafür ist Wissen darüber, welche Kooperationsangebote Eltern von Schulen zum Thema digitale Medien erhalten. Die vorliegende Studie untersuchte anhand einer Befragung von N = 736 Schülerinnen und Schülern der fünften Klassenstufe und N = 426 ihrer Eltern, welche Informations- und Teilhabeangebote letztere erhielten und wie diese Angebote mit elterlichen Fähigkeiten zur Nutzung und Gestaltung des Internets sowie Medienerziehung zusammenhängen. Eltern erhielten nur zum Teil Informations- und Teilhabeangebote zum Thema ‹digitale Medien›. Eltern mit Migrationshintergrund und Eltern mit niedrigeren bis mittleren Bildungsabschlüssen berichteten aber, häufiger Angebote zu erhalten. Je häufiger Eltern Angebote erhielten, bei denen sie digitale Medien mit ihren Kindern zusammen ausprobieren und erproben konnten, umso geringer waren Fähigkeiten zur Internetnutzung. Dies kann mit der Passung der Angebote von Schulen zu den Bedürfnissen von Eltern zusammenhängen, aber auch ein Anzeichen dafür sein, dass gerade Eltern mit geringeren Fähigkeiten auf Kooperationsangebote von Schulen eingehen. Es wurden keine signifikanten Zusammenhänge zwischen Kooperationsangeboten und Medienerziehung gefunden. Ergebnisse können helfen, vermehrte Angebote für Eltern zu schaffen sowie adressatengerecht zu gestalten.
Gerenciamento de resultados e de capital por bancos latino-americanos com instrumentos financeiros
Authors: Alves de Carvalho José , César Gomes Mendonça Júlio , Júnior Maurício Soares de Faria , Alves Dantas José 
Published: January 2022

Objetivo: O objetivo do estudo foi avaliar se os bancos da América Latina utilizam os ganhos e perdas não realizados com instrumentos financeiros, registrados como outros resultados abrangentes, com o objetivo de gerenciamento de resultados e de capital regulatório.
Metodologia: Utilizando dados de 75 bancos de 13 países da América Latina, de 2008 ao primeiro trimestre de 2020, os testes empíricos compreenderam a estimação de modelo de dados em painel com efeitos fixos seccionais, seguindo Barth et al. (2017).
Resultados: As estimações realizadas revelaram não haver evidências empíricas de que esses bancos, de forma geral, utilizem ganhos e perdas não realizados com instrumentos financeiros para fins de gerenciamento de resultados. Por outro lado, os resultados empíricos revelam que os bancos com menores índices de capital (menor quintil) utilizam essas parcelas de ganhos e perdas com os instrumentos financeiros DPV ou VJORA, tanto como mecanismo de gerenciamento de resultados quanto de capital.
Contribuições do Estudo: O estudo contribui para o avanço da literatura contábil sobre gerenciamento de resultados, muitas vezes restritos ao uso dos accruals discricionários, ao avaliar a utilização da abordagem de gerenciamento de resultados e de capital por meio de atividades operacionais – no caso do gerenciamento de capital, por meio da redução dos ativos ponderados pelo risco (RWA).
Impacto da exclusão do ICMS da base de cálculo do PIS e da COFINS: estudo sobre a decisão do STF e seus efeitos em uma empresa do segmento alimentício
Authors: Andressa Zago Jéssica , Twardowski da Silva Letícia , Paulo Rigo Vitor 
Published: January 2022

Objetivo: O objetivo do estudo foi apresentar o impacto da decisão proferida pelo Supremo Tribunal Federal que exclui o ICMS da base de cálculo do PIS e da COFINS e demonstrar o montante dessas contribuições a serem restituídas por uma empresa do setor alimentício, enquadrada no regime do Lucro Real.
Metodologia: Utilizou-se uma pesquisa descritiva, aplicada a um estudo de caso com abordagem quantitativa dos dados. A composição dos valores e identificação do indébito foram feitos a partir da análise minuciosa dos documentos fiscais e contábeis da empresa, dos anos de 2012 até 2017.
Resultados: Verificou-se que a decisão do STF permite interpretações distintas quanto a sistemática de cálculo para ressarcimento de eventuais valores, portanto como ainda não há segurança jurídica quanto ao critério a ser utilizado, optou-se por demonstrar os valores pelos dois métodos, ou seja, excluindo o ICMS destacado e o ICMS recolhido. O resultado da pesquisa evidenciou que ambas as formas de cálculo se mostraram benéficas para a empresa, mesmo com a enorme discrepância identificada se confrontados os valores de indébito encontrados em cada critério de cálculo.
Contribuições do Estudo: O estudo traz uma contribuição significativa pois trata-se da decisão proferida pelo STF que exclui o ICMS da base de cálculo do PIS e COFINS, assunto esse que arrastava-se pelo STF há muitos anos, e que ainda se mostra confusa e permite interpretações distintas quanto ao método de cálculo do indébito. As decisões desarmonizadas e inseguras, os grandes valores que o governo necessita restituir às empresas e a amplitude que essa decisão tomou em âmbito nacional tornam o assunto relevante pois como mostrado no presente estudo, apesar dos resultados discrepantes entre as formas de apuração do valor a restituir/compensar, a empresa obtém expressiva vantagem tributária.