bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barrier Perturbation in Porcine Peyer’s Patches by Tumor Necrosis Factor is Associated With a Dysregulation of Claudins
Authors: Droessler Linda , Cornelius Valeria , Boehm Elisa , Stein Laura , Brunner Nora , Amasheh Salah 
Published: May 2022

The proinflammatory cytokine tumor necrosis factor (TNF) has been described as one of the main mediators of intestinal inflammatory diseases, affecting the composition of tight junction (TJ) proteins and leading to a disruption of the epithelial barrier. An intact intestinal barrier is mandatory, because the follicle-associated epithelium of Peyer’s patches represents the first defense line of the intestinal immune system and ensures a controlled uptake of antigens from the gut lumen. In the current study, we have analyzed the detailed effects of TNF on the follicle-associated epithelium of porcine Peyer’s patches by applying the Ussing chamber technique. Epithelial tissue specimens of Peyer’s patches and the surrounding villus epithelium were mounted into conventional Ussing chambers and incubated with TNF for 10 h. The transepithelial resistance, representing epithelial barrier function of the tissue, was recorded. A reduction of transepithelial resistance was detected after 8 h in Peyer’s patch tissue specimens, whereas the villus epithelium was not significantly affected by TNF. Subsequent molecular analysis of TJ protein expression revealed a marked decrease of claudin-1 and -4, and an increase of claudin-2. In neighboring villus epithelium, no significant changes in the expression of TJ proteins could be shown. A strong increase of TNF receptor-2 (TNFR-2) could also be detected in Peyer’s patches, in agreement with the major role of this receptor in Peyer’s patches. Our findings were in accordance with changes detected by confocal laser scanning immunofluorescence microscopy. The regulation of TNF effects via myosin light chain kinase (MLCK) was analyzed in blocking experiments. Our detailed analysis is the first to show that TNF affects the barrier function of the follicle-associated epithelium of porcine Peyer’s patches but has no effects on the villus epithelium. These findings reveal not only the basic differences of epithelial barrier function between the two structures, but also the significance of Peyer’s patches as a primary mucosal immune defense.
The Impact of Prepartum Depression and Birth Experience on Postpartum Mother-Infant Bonding: A Longitudinal Path Analysis
Authors: Eitenmüller Pia , Köhler Siegmund , Hirsch Oliver , Christiansen Hanna 
Published: May 2022

IntroductionNegative effects of impaired postpartum mother-infant-bonding on mental health of mothers, their newborn children and subsequent child development are well documented. Previous research demonstrated an association between a negative birth experience and postpartum mental health affecting postpartum mother-infant bonding. This study investigates the extent to which prepartum depression and birth experience influence the postpartum mental health of mothers and their bonding toward their newborns, and whether these influences differ according to parity and self-reported prior mental health problems.MethodThree hundred and fifty-four women (18-43 years;M= 30.13,SD= 5.10) filled in the Edinburgh Postnatal Depression Scale (EPDS), the Maternal-Fetal Attachment Scale (MFAS), Salmon's Item List (SIL) assessing the birth experience, and the Postpartum Bonding Questionnaire (PBQ) at pre- and postpartum; they were also asked about birth complications and parity status.ResultsPrimipara reported significantly more birth complications (p= 0.048), with path analysis confirming this result (p&lt; 0.001). Birth complications were associated with a more negative rating of the overall birth experience (p&lt; 0.001). Mothers with self-reported prior mental health problems had higher prepartum depression scores (p&lt; 0.001) but did not differ in other variables from mothers without prior self-reported mental health problems. Differences in depression scores between mothers with self-reported prior mental health problems and those without vanished at postpartum assessment (p&gt; 0.05). Path-analysis highlighted the key role of postpartum depression, which was the only significant predictor of postpartum impairment in maternal-child bonding (p&lt; 0.001). Birth experience and prepartum depression scores exerted an indirect effect on postpartum maternal-child bonding, mediated by postpartum depression.DiscussionThe present study demonstrates the relevance of prepartum mental health of expectant mothers, especially of those who self-report prior mental health problems. The results support that reducing mental health problems of pregnant mothers might contribute to a more positive birth experience and potentially reduce postpartum depressive symptoms. As postpartum depression is associated with impaired parent-child bonding, such targeted interventions could promote child development. Group differences between primiparous and multiparous mothers suggest that the birth experience may be an influential factor for postpartum mental health.
Comparison of Effects of Mothers' and Mozart's Lullabies on Physiological Responses, Feeding Volume, and Body Weight of Premature Infants in NICU
Authors: Shin Hyo-Jin , Park Jooyeon , Oh Hye-Kyung , Kim Nahyun 
Published: May 2022

ObjectivesThe purpose of this study was to compare the effects of mothers' and Mozart's lullabies on physiological parameters, feeding volume, and body weight of premature infants in a neonatal intensive care unit (NICU).MethodsThis study used a non-equivalent control group, non-synchronized design as a quasi-experimental study. Two intervention groups (recorded mother's lullaby and Mozart's lullaby) and a control group were formed from a total of 65 premature infants: a mother's lullaby group of 22 infants, a Mozart's lullaby group of 22 infants, and a control group of 21 infants not provided with any lullabies. Their physiological parameters included heart rate, blood pressure, respiratory rate, and O2 saturation. The infants' feeding volume and body weight were measured as indicators related to the growth of premature infants. The mother's and Mozart's lullabies were played on a speaker in an incubator for 15 min for 7 consecutive days per group.ResultsThere were significant differences in the mean difference before and after intervention in neonatal heart rate (χ2 = 45.03, P &lt; 0.001), systolic pressure (F = 43.29, P &lt; 0.001), diastolic pressure (χ2 = 33.01, P &lt; 0.001), respiratory rate (F = 76.06, P &lt; 0.001), and O2 saturation (χ2 = 40.82, P &lt; 0.001) between the three groups. The mean differences of both mother's and Mozart's lullaby groups were significantly higher than those of the routine care group in all physiological parameters, and those of the mother's lullaby group was significantly higher when compared with the Mozart's lullaby group. In repeated-measures ANOVA, there was a significant interaction between time and group in feeding volume (F = 2.46, P = 0.041). However, body weight did not significantly differ in an interaction between time and group (F = 1.75, P = 0.151).ConclusionThis study showed beneficial effects of mother's lullaby and Mozart's lullaby on physiological parameters. Especially, the mother's lullaby was found to significantly improve all physiological parameters and feeding volume of premature infants in the NICU compared to Mozart's lullaby group. Therefore, we recommend the regular integration of the mother's lullaby into supportive care of premature infants in the NICU, as this intervention highlights the need for mothers to participate in their care.Trial RegistrationClinicalTrials.gov, identifier: KCT0004842 (https://cris.nih.go.kr).
Failure to Launch: The Endocrine Disruptor Screening Program at the U.S. Environmental Protection Agency
Authors: Maffini Maricel V. , Vandenberg Laura N. 
Published: May 2022

It has been 25 years since the U.S. Congress passed the Food Quality Protection Act of 1996, an amendment to the Food Drug and Cosmetic Act, which mandated that the US Environmental Protection Agency (EPA) test all pesticide chemicals used in food for endocrine disruption. Soon after the law passed, EPA established the Endocrine Disruptor Screening and Testing Advisory Committee (EDSTAC) to provide recommendations to the agency on how its Endocrine Disruptor Screening Program (EDSP) should work. Among them, the committee recommended that EDSP screening should 1) evaluate both human and ecological effects; 2) test for disruption of the estrogen, androgen, and thyroid systems; 3) evaluate pesticide and non-pesticide chemicals; and 4) implement a tiered approach. EPA adopted the recommendations and the EDSP was created in 1998. To date, the EPA has yet to fully implement the law; in other words, it has failed to test all pesticide chemicals for endocrine disruption. Of the small number that have been tested, not a single pesticide chemical has been determined to be an endocrine disruptor, and no regulatory actions have been taken. Here, we review the missed opportunities EPA had to make the EDSP a functional and effective program aimed at protecting human health and the environment. Two reports by the EPA’s Office of Inspector General from 2011 to 2021 provide the framework for our discussion.
Serological Variety and Antimicrobial Resistance in Salmonella Isolated from Reptiles
Authors: Merkevičienė Lina , Butrimaitė-Ambrozevičienė Česlova , Paškevičius Gerardas , Pikūnienė Alma , Virgailis Marius , Dailidavičienė Jurgita , Daukšienė Agila , Šiugždinienė Rita , Ruzauskas Modestas 
Published: May 2022

Salmonella enterica is one of the best adapted bacterial pathogens causing infections in a wide variety of vertebrate species. The aim of this study was to investigate the prevalence of Salmonella in different reptile species and to evaluate their serological variety and patterns of antimicrobial resistance. In total, 97 samples from 25 wild and domesticated reptile species were investigated in Lithuania. Serological variety, as well as phenotypical and genotypical resistance to antimicrobials, were investigated. Fifty isolates of Salmonella were obtained from the ninety-seven tested samples (51.5%; 95% CI 41.2–61.2). A significantly higher prevalence of Salmonella was detected in domesticated individuals (61.3%; 95% CI 50.0–71.5) compared with wild ones (18.2%; 95% CI 7.3–38.5). All isolates belonged to a single species, Salmonella enterica. Results demonstrated that reptiles carry a large variety of Salmonella serovars. Thirty-four isolates (68%) of Salmonella were resistant to at least one antimicrobial drug. The most frequent resistance of the isolates was to streptomycin (26%), cefoxitin, gentamicin, tetracycline and chloramphenicol (16%). Genes encoding resistance to tetracyclines, aminoglycosides, sulphonamides and trimethoprim were detected. No integrons that are associated with horizontal gene transfer were found. Data obtained provided knowledge about the adaptation of Salmonella in reptiles. Healthy individuals, irrespective of their origin, often carry Salmonella, including multi-resistant strains. Due to its large serological diversity, zoonotic potential and antimicrobial resistance, Salmonella in reptiles poses a risk to other animals and humans.
Design, Synthesis, In Vitro Biological Activity Evaluation and Stabilized Nanostructured Lipid Carrier Formulation of Newly Synthesized Schiff Bases-Based TMP Moieties
Authors: Bukhari Syed Nasir Abbas , Zakaria Mohamed Y. , Munir Muhammad Usman , Ahmad Naveed , Elsherif Mervat A , Badr Rasha Emad , Hassan Ahmad Khalaf , Almaaty Ali H. Abu , Zaki Islam 
Published: May 2022

A series of novel Schiff bases-based TMP moieties have been designed and synthesized as potential anticancer agents. The target Schiff bases were screened for their cytotoxic activity against the MDA-MB-231 breast cancer cell line. Most of the tested molecules revealed good cytotoxic activity, especially compounds 4h, 4j and 5d. Being the most potent, compound 4h showed good tubulin polymerization inhibition activity as revealed by immunofluorescence analysis and ELISA assay. Additionally, compound 4h was screened for cell cycle disturbance and apoptosis induction. Pre-G1 apoptosis and cell growth halt at the G2/M phase were discovered to be caused by it. Moreover, compound 4h induced apoptosis via p53 and Bax activation, as well as reduced the level of Bcl-2. Additionally, the most potent compound 4h was lodged on nanostructured lipid carriers (NLCs). 23 full factorial design was involved to govern the influence of the fabrication variables on the in vitro characters of the casted NLCs. F3 was picked as the optimum formula exhibiting dominant desirability value 0.805, EE% 95.6 ± 2.4, PS 222.4 ±18.7, PDI 0.23 ± 0.05 and ZP −39.2 ± 3.9 Mv. Furthermore, F3 affirmed improved solubility and release over the drug suspension. In the comparative cytotoxic activity, F3 was capable of diminishing the IC50 by around 2.15 times for pure 4h, while nearly close to the IC50 of the reference drug. Thus, NLCs could be a potential platform for boosted antitumor activity.
Biorecovery of Agricultural Soil Impacted by Waste Motor Oil with Phaseolus vulgaris and Xanthobacter autotrophicus
Authors: Martínez Blanca Celeste Saucedo , Benavides Liliana Márquez , Santoyo Gustavo , Sánchez-Yáñez Juan Manuel 
Published: May 2022

Agricultural soil contamination by waste motor oil (WMO) is a worldwide environmental problem. The phytotoxicity of WMO hydrocarbons limits agricultural production; therefore, Mexican standard NOM-138-SEMARNAT/SSA1-2012 (NOM-138) establishes a maximum permissible limit of 4400 ppm for hydrocarbons in soil. The objectives of this study are to (a) biostimulate, (b) bioaugment, and (c) phytoremediate soil impacted by 60,000 ppm of WMO, to decrease it to a concentration lower than the maximum allowed by NOM-138. Soil contaminated with WMO was biostimulated, bioaugmented, and phytoremediated, and the response variables were WMO concentration, germination, phenology, and biomass of Phaseolus vulgaris. The experimental data were validated by Tukey HSD ANOVA. The maximum decrease in WMO was recorded in the soil biostimulated, bioaugmented, and phytoremediated by P. vulgaris from 60,000 ppm to 190 ppm, which was considerably lower than the maximum allowable limit of 4400 ppm of NOM-138 after five months. Biostimulation of WMO-impacted soil by detergent, mineral solution and bioaugmentation with Xanthobacter autotrophicus accelerated the reduction in WMO concentration, which allowed phytoremediation with P. vulgaris to oxidize aromatic hydrocarbons and recover WMO-impacted agricultural soil faster than other bioremediation strategies.
Affective Learning Strategy and Lecturers’ Exemplary: Do They Affect in Shaping Scholars’ Character?
Authors: Mujiyanto Mujiyanto 
Published: May 2022

Factors affecting students' character are very various. In this case, it needs a certain study that reveals several specific factors affecting students' character. The objectives of this study is to analyze the influence of affective learning strategies and Lecturers' Exemplary on shaping students' character. The research uses quantitative methods with multiple regression research. The research population was all students of the Buddhist Religious Education Study Program, Raden Wijaya State Buddhist college with the sample used was 33 students. Data collection techniques were carried out by questionnaires. The results of the analysis show that partially affective learning strategies have a significant positive effect in shaping students’ character with a value of sig (p) 0.05 or t obtained t table (6,485 2,045). There is a significant positive effect among lecturers’ exemplary in shaping students’ character with a value of sig (p) 0.05 or t obtained t table (5.566 2.045). Moreover, affective learning strategies and lecturers' exemplary influence simultaneously in shaping students’ character, it is because of the value of sig 0.00 (p) 0.05. Furthermore, the effect is amount to 94.2% and 5.8% is influenced by other variables that have not been studied. Impliciation of this research is that lecturers should keep their attitude, utterance, and behaviour especially in the class because the students tend to imitate their lecturers.
Rationale for the use of a novel structure device for mandible fractures fixation
Authors: Taraev A.Yu. , Ushakov R.V. 
Published: May 2022

The finite element method in the Ansys Workbench system was used to solve static problems of the theory of elasticity, simulating the stress-strain state of the “bone-fixing device” systems under the action of the chewing, temporal, lateral pterygoid, and medial pterygoid muscles. The stability of each of the considered fixing devices was quantitatively assessed, the relative displacements of bone fragments, equivalent stresses in the bone and fixing devices were calculated and compared, and the contact pressure between bone fragments was investigated.
It is shown that with the considered oblique fracture and the considered loads, the fixation device of a new type provides similar stability in comparison with the 3D plate. It was revealed that the fixation device of a new type, in comparison with the 3D plate, provides a higher contact pressure and a tighter contact between fragments. Equivalent stresses in the bone structures during the installation of the new type of fixation device were found to be lower than in similar bone structures during the installation of the 3D plate.
Dissolved Oxygen in the East Bolaang Mongondow Waters, North Sulawesi
Authors: Patty Simon I. , Rizqi Marenda Pandu , Huwae Rikardo 
Published: May 2022

Oxygen in seawater comes from the air through diffusion and photosynthesis. This study aims to determine the dissolved oxygen content, NAEC (Normal Atmospheric Equilibrium Concentration), and AOU (Apparent Oxygen Utilization) in East Bolaang Mongondow waters. The analysis showed that the dissolved oxygen content in the surface layer ranged from 6.01-7.05 mg/l; 5.91-6.98 mg/l (5 m depth); and 5.75-6.90 mg/l (near bottom depth). NAEC is closely related to water temperature and implicitly increases with depth. The highest NAEC value of 5.90 mg/l (t=28.0ºC) was found at the bottom depth, and the lowest NAEC of 5.63 mg/l (t=30.0ºC) was found at the surface layer. The dissolved oxygen saturation level of more than 100% and the average positive AOU value at 0.59-0.81 mg/l describe the amount of oxygen available in the surface layer.Keywords: Dissolved Oxygen; Normal Atmospheric Equilibrium Concentration (NAEC); Apparent Oxygen Utilization (AOU); East Bolaang Mongondow.AbstrakOksigen dalam air laut bersumber dari udara melalui proses difusi dan hasil fotosintesis. Penelitian ini bertujuan untuk mengetahui kandungan oksigen terlarut, NAEC (normal atmospheric equilibrium concentration) dan AOU (apparent oxygen utilization) di perairan Bolaang Mongondow Timur. Hasil analisis menunjukkan bahwa kandungan oksigen terlarut di lapisan permukaan berkisar antara 6,01-7,05 mg/l; kedalaman 5 meter 5,91-6,98 mg/l dan dekat dasar 5,75-6,90 mg/l. NAEC sangat berhubungan dengan suhu air dan secara implisit konsentrasinya akan semakin tinggi dengan bertambahnya kedalaman. Nilai NAEC tertinggi 5,90 mg/l (t=28,0ºC) ditemukan pada kedalaman dekat dasar dan NAEC terendah 5,63 mg/l (t=30,0ºC) ditemukan pada lapisan permukaan. Tingkat kejenuhan oksigen terlarut >100 % dan rata-rata nilai AOU positif (0.59-0.81 mg/l) menggambarkan banyaknya kandungan oksigen yang tersedia pada lapisan permukaan.Kata kunci: oksigen terlarut; normal atmospheric equilibrium concentration (NAEC); apparent oxygen utilization (AOU); Bolaang Mongondow Timur.
Biodiversidad de equinodermos en Bahías de Papanoa
Authors: Lechuga Granados Adriana , Arroyo Vega César 
Published: May 2022

México es el hábitat del 10% de especies de equinodermos en el planeta, el Pacífico Mexicano es importante ya que alberga un 52% de las especies registradas para México, además de especies endémicas que comparte con el Indo-Pacífico. El estado de Guerrero presenta 135 especies de equinodermos, mientras que Oaxaca 94 y Chiapas 15, siendo es el estado con mayor diversidad al presentar mayor número de especies en comparación con los estados del Pacifico Sur Mexicano, sin embargo, tanto sus áreas terrestres como acuáticas han sido poco estudiadas y especialmente en el grupo de los equinodermos. El objetivo del presente estudio es conocer la diversidad de especies de equinodermos que habitan en Bahías de Papanoa, Guerrero-México, comprende un total de once sitios de recolecta, cuenta con una compleja variedad de ambientes como arrecifes coralinos, fondos arenosos y rocosos en una longitud lineal aproximada de 15 km. Durante un año de muestreos mensuales, se utilizaron técnicas de captura directa, búsqueda de barrido, aleatorio y transecto, con buceo Scuba y libre, en inmersiones de los 0 a 20 metros de profundidad en un rango de temperatura del agua marina de 27 a 31°C. Se registran 55 especies de equinodermos, tales como: Ophiocoma sp, Phataria sp, Pentaceraster sp, Cucumaria sp, Astropyga sp, Meoma sp, Diadema sp, Afrocucumis ovulum, Pseudocnus californicus, Neocucumis veleronis y Holothuria imitans. La biodiversidad de equinodermos en la complejidad de sus diversos ambientes y sustratos en los 15 km de longitud estudiados en Bahías de Papanoa, señalan que esta localidad en el estado de Guerrero existe la posibilidad de nuevas especies, esta zona representa el 3% de litoral marino para todo el estado de Guerrero, alberga 34.5% de los equinodermos del Pacifico Sur Mexicano, y tan solo en Bahías de Papanoa se encuentra el 58.3% de especies respecto a las 96 especies de los ejemplares depositados en la Colección Nacional de Equinodermos (CNE-ICMyL-UNAM) para todo el estado de Guerrero. Con estos resultados, resulta prioritario dar continuidad a estudios sobre, taxonomía, distribución, ecología, sistemática molecular, diversidad, fisiología, recurso pesquero, entre otros, en esta importante zona del Pacifico Sur Mexicano.

Palabras clave: Echinodermata; Guerrero; Bahías de Papanoa.
Elucidation of electrical properties of undoped and Sb-induced Cu2SnS3 (CTS) thin films and degradation properties on CTS thin films and solar cells by intentional proton irradiation
Authors: Urata Nanami , Kanai Ayaka , Sugiyama Mutsumi 
Published: May 2022

Abstract
The fundamental electrical properties of undoped and Sb-induced Cu2SnS3 (CTS) thin films were evaluated. Furthermore, the relationship between defect properties during intentional degradation and thin film/solar cell properties was investigated. The carrier concentration decreased after Sb induction in the CTS film, and the resistivity increased by one order of magnitude. These values were independent of the Sb volume. These results imply that a small quantity of Sb atoms passivates the defects, such as Sb atoms at Sn or Cu sites that compensate for the intrinsic acceptors at Cu vacancies. In addition, the number of defects around the grain boundary tended to decrease with Sb induction because of passivation. The carrier concentration of the CTS layer remained unchanged following proton irradiation at 1 × 1014 cm−2. Furthermore, the number of defects increased, independent of the Sb induction.
A high sensitivity and strong stability miniaturized optically-pumped magnetometer
Authors: Wu Zinan , Zhang Yang , He Mengyang , Ren Bokang , Yue Huimin , Ou Zhonghua , Zhou Xiaojun , Liu Yong 
Published: May 2022

Abstract
In this paper, we focus on the phase noise in optically-pumped magnetometer systems and investigate its sources, and use dual-phase lock-in technique to solve the problem. The results show that the phase noise is maximum when the system operates under zero-magnetic-field condition, and the main source comes from the environment stray magnetic field. The dual-phase lock-in technique improves the phase noise rejection by a factor of 10 compared to the single-phase lock-in technique. The sensitivity of the system is 22 fT Hz−1/2 and the bandwidth is 158 Hz, which offers a favorable outlook for use in clinical settings.