bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conduta de Autolesão não Suicida em Adolescentes
Authors: Da Silva Marcelo Gonçalves , Pereira Ana Carla Tamisari , Roques  Dauzacker Robson Adriani , Maciel Garcia Eloy Aparecido , Fonseca Wanaline , Bernal Jaqueline , Santos Viviane Silva , Luzi Sandra Lopes 
Published: July 2022

A autolesão não suicida na adolescência tem recebido atenção de pesquisadores em virtudedo aumento de casos relatados nas escolas e mídias sociais, porém, o tema ainda é pouco focalizado na produção científica. Por tanto, este estudo tem objetivo de investigar, quais são as características, nos aspectos modos de conduta, sociodemográficos e psicológicos de pessoas, sem psicopatologia, que apresentam conduta autolesiva não suicida. Trata-se de um estudo qualitativo realizado através de estudos publicados nas bases de dados Scientific Eletronic Library (SciELO), Portal de Periódicos Eletrônicos de Psicologia (PePSIC) e no Catálogo de Teses e Dissertações, utilizando os descritores: autolesão, automutilação, adolescência, em diversas combinações de artigos de pesquisas de campo com o acesso liberado entre os anos de 2014 a 2019. Discute-se que a autolesão em adolescentes precisa ser compreendida como sendo configurada a partir dos condicionantes histórico-sociais que permeiam a experiência do que é ser adolescente na sociedade atual e torna-se importante que o psicólogo escolar atue considerando as conjunturas presentes na atualidade e que sua ação se volte para finalidades transformadoras. A população sem psicopatologia que apresenta autolesão não suicida são pessoas que se cortam na superfície da pele, podendo variar, na fase da adolescência com idades entre dez e dezoito anos, de classe baixa e média, morando com um dos progenitores, vivenciam fortes conflitos e emoções adversas e se lesionam no intuído de se sentirem melhor.
Evaluation of the relationship between Doppler predictors with human papillomavirus types, smear and cervical biopsy results
Authors: TURHAN ÇAKIR Anıl , ARSLAN Aydan 
Published: July 2022

Objectives: The study aims to investigate whether there is any difference between human papillomavirus (HPV) types, smear results, and colposcopic biopsy results in terms of iliac, uterine, and cervical artery pulsatility and resistive index values in high-risk HPV positive patients.Methods: Iliac, uterine, and cervical artery pulsatility and resistive index values were determined by pelvic Doppler ultrasonography in patients who applied for high-risk HPV positivity and underwent colposcopyguided cervical biopsy. Results: There was no difference between HPV types and Pap-smear results and the pulsatility and resistive indices of the iliac artery, uterine artery, and cervical artery. It was observed that the mean cervical artery pulsatility index of the patients whose colposcopic cervical biopsy result was cervical intraepithelial neoplasia (CIN) 1 was 1.61 ± 0.43 and the cervical artery pulsatility index of the patients with CIN 2-3 was 1.15 ± 0.28, and a statistically significant difference was found between them (p = 0.038). There was no difference between other Doppler indices and colposcopic cervical biopsy results. Conclusions: Doppler indices such as cervical artery pulsatility index may be helpful in the evaluation of cervical cancer precursor lesions.
PHYTOCHEMICAL AND MICROBIOLOGICAL ASPECTS OF THE <i>NIGELLA SATIVA</i> L. HERBS STUDY
Authors: Mubinov A. R. , Kurkin V. A. , Avdeeva E. V. , Kolpakova S. D. , Zhestkov A. V. 
Published: July 2022

Nigella sativa L. is a promising plant object, herbal medicinal raw materials of which can be comprehensively used for the development of drugs with an antimicrobial activity.The aim of the study was to screen and compare the antimicrobial activity of water-ethanolic extractions from the Nigella sativa L. herbs with a eucalyptus tincture of as a reference preparation.Materials and methods. Chromatograms of the extracts were obtained by thin layer chromatography in the system of chloroform – ethanol – water (26:16:3). The detection of adsorption zones was carried out in daylight, in the UV light at λ=254 nm and λ=365 nm, as well as by treatment with reagents – a 3% alcohol solution of aluminum chloride and a solution of diazobenzosulfonic acid in a 20% sodium carbonate solution. The next step was to determine the minimum inhibitory concentration by the method of double serial dilutions in Mueller-Hinton nutrient broth (Bio-Rad, USA). As test cultures, the strains of the American Type Culture Collection (ATCC) microorganisms were used: Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), as well as Candida albicans (a clinical strain). Simultaneously, an experiment to establish a “negative” control was carried out. For the comparative evaluation of the studied samples activity, its activity was compared with the reference preparation with a proven antimicrobial activity – a eucalyptus tincture.Results. For all water-ethanolic extractions and the Nigella sativa L. herb tincture, the adsorption zones characteristic of flavonoids with Rf1 = 0.28, Rf2 = 0.15, Rf3 = 0.11 were revealed, and under the action of an alcoholic solution of aluminum chloride, the fluorescence of the adsorption zones was also enhanced, which indicates the phenolic nature of these compounds.In the course of the study, it was found out that all water-ethanolic extractions from the Nigella sativa L. herbs have the greatest antimicrobial effect against the Pseudomonas aeruginosa strain. When compared with the reference preparation – a eucalyptus tincture, it was notified that the specified tincture of the Nigella sativa L. herbs has an advantage in the antimicrobial activity over the strain of Pseudomonas aeruginosa – the action at the 16-fold dilution vs the 4-fold dilution. The action on the Escherichia coli and Candida albicans strains is comparable for the both tinctures.Conclusion. The obtained results of phytochemical and microbiological analyses will be used as a rationale for the introduction of antimicrobial preparations based on the Nigella sativa herbs in medical and pharmaceutical practice.
No Difference in Conversion Rate to Hip Arthroplasty After Intramedullary Nail or Sliding Hip Screw for Extracapsular Hip Fractures
Authors: Pyrhönen Helmi-Sisko , Lagergren Johan , Wolf Olof , Bojan Alicja , Mukka Sebastian , Möller Michael , Rogmark Cecilia 
Published: July 2022

Background:
The widespread use of intramedullary nails (IMNs) compared with sliding hip screws (SHSs) in extracapsular hip fractures (AO/OTA 31-A1, 31-A2, 31-A3) has been questioned because of a higher complication rate, although the outcome might have improved through more recent implant designs and the learning curve. This study aimed to investigate if there is a difference with regard to the cumulative incidence of conversion to arthroplasty or any reoperation during the first 5 years after IMN or SHS fixation of extracapsular hip fractures.

Methods:
In this nationwide, observational cohort study, individuals who were ≥60 years of age and were registered in the Swedish Fracture Register (SFR) from 2012 to 2018 due to extracapsular fracture and were primarily treated with an IMN or SHS were followed in the SFR and the Swedish Arthroplasty Register (SAR) for a minimum of 1 year. The primary outcome was the cumulative incidence of conversion to arthroplasty (conversion rate). The secondary outcome was the cumulative incidence of all reoperations (reoperation rate). Both were calculated in a competing risk analysis during the first 5 years.

Results:
We included 19,604 individuals (70% women), with a median age of 85 years (range, 60 to 107 years). The 31-A2 fracture was most prevalent (52%), followed by the 31-A1 fracture (28%). No significant differences were seen in the 1-year conversion rate after IMN or SHS use (1.0% compared with 0.9% in the 31-A1 fractures, 1.7% compared with 1.3% in the 31-A2 fractures, and 1.3% compared with 1.5% in the 31-A3 fractures) or in the 1-year reoperation rate (1.9% compared with 1.9% in the type-A1 fractures, 3.4% compared with 2.5% in the type-A2 fractures, and 4.0% compared with 5.2% in the type-A3 fractures). Only in 31-A2 fractures were more reoperations seen after IMN use at 2 and 5 years (p < 0.05). The crude 1-year-mortality was 26.4% (5,178 of 19,604), without significant differences between implants.

Conclusions:
Considering conversion arthroplasty, IMNs and SHSs performed equally well in general. IMN use was associated with more reoperations than SHS use in 31-A2 fractures at 2 years. However, from a clinical perspective, the differences between the implants were small, in particular when considering the competing risk of dying.

Level of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
FIRST SYMBIOTIC ASSOCIATION BETWEEN HEDERELLOIDS AND RUGOSE CORALS (LATEST SILURIAN OF SAAREMAA, ESTONIA)
Authors: VINN OLEV , WILSON MARK A. , MADISON ANNA , KAZANTSEVA ELIZAVETA , TOOM URSULA 
Published: July 2022

ABSTRACTA phoronid-like hederelloid that formed a symbiotic association with the rugosan coral Entelophyllum has been found in the Pridoli Series of Estonia. The skeletons of the hederelloid and rugosan are partially intergrown. The hederelloid apertures are located at the margin of the rugosan calice. The hederelloid lophophore was likely placed between the tentacles of the coral polyp, indicating a positive or at least a neutral co-existence. In extant corals, polyps can be retracted into the calice either during the day or night. If the rugose coral was similar to modern corals in this respect, it may have helped in the co-existence with the hederelloid and allowed feeding at different times. It is possible that the hederelloid soft tissues were protected against smaller predators by the stinging cells of the rugosan tentacles. The history of symbiosis in hederelloids is similar to that of tentaculitoids, in which symbiotic relationships also appeared early in their evolution. To date, Entelophyllum is the only colonial rugosan known to have formed symbiotic associations during the Silurian.
TMBIM1 promotes EMT by stimulating autophagic degradation of E-cadherin via AMPK/mTOR/ULK1 axis in human gliomas
Authors: Gao Lun , Liu Junhui , Zhang Shenqi , Li Yong , Tan Yinqiu , Yang Ji’an , Cai Jiayang , Wang Long , Ye Zhang , Tong Shi’ao , Deng Gang , Cai Qiang , chen qianxue 
Published: July 2022

Abstract
Glioblastoma (GBM, WHO grade IV) is well known for its highly metastatic and recurrent and accounts for approximately 50% of all gliomas. Dysregulation of epithelial–mesenchymal transition (EMT) can lead to malignant progression of GBM. Therefore, it is urgently needed to illustrate the mechanisms by which molecular drivers the EMT in GBM. We found for the first time that Transmembrane BAX inhibitor motif-containing 1 (TMBIM1) was over-expressed in gliomas compared with non-tumor brain tissues and increased as glioma malignancy progressed. Patients with high TMBIM1 expression had shorter overall survival time than those with low TMBIM1 expression. Importantly, TMBIM1 obviously induced EMT and autophagy, and inhibition of autophagy could reverseTMBIM1-induced EMT both in vitro and in vivo assays. TMBIM1 induced EMT by downregulating E-cadherin expression, which was proceeded by inhibiting the autophagic degradation of E-cadherin. Inhibition of TMBIM1 dramatically decreased the expression of P-AMPKα Thr172 and P-ULK1 Ser317 in U87 and U251 cells, and upregulated the expression of P-mTOR at Ser2448. We revealed that TMBIM1 enhanced the EMT and promoted autophagic degradation of E-cadherin via AMPK/mTOR/ULK1 axis both in vitro and in vivo. Our study provides a novel mechanism for the regulation of EMT in the process of gliomas metastasis, indicating that suppression of TMBIM1 activity to attenuate autophagy may be a potential strategy for the treatment of gliomas.
Construction of Azaheterocycles via Pd-Catalyzed Migratory Cycloannulation Reaction of Unactivated Alkenes
Authors: Wang Jin-Ping , Song Shuo , Wu Yichen , Wang Peng 
Published: July 2022

Abstract
Azahetereocycles constitute important structural components in many biologically active natural compounds and marketed drugs, and represent the most promising scaffolds in drug discovery. Accordingly, the development of efficient and general synthetic methods for the construction of diverse azaheterocycles is the major goal in synthetic chemistry. Although there have been notable advances in transition metal catalyzed formal [n+2] reaction with alkenes in which the cyclization normally occurred in the vicinal 1,2-position of those alkenes, significantly fewer migratory cycloannulation processes have been reported due to the low reactivity of the unactivated nonconjugated alkenes, unavoidable Heck-type byproducts, and the difficulties for controlling the cyclization position along the carbon chain of the alkenes during the metal migratory process. Herein, we report the efficient construction of a wide range of azaheterocycles via a Pd-catalyzed migratory cycloannulation strategy with unactivated alkenes by overcoming aforementioned limitations. This novel strategy enables the rapid construction of a series of 6-, 7- and 8-membered azaheterocycles in high efficiency, and features broad substrate scope, excellent functional group tolerance under redox-neutral conditions. The significance of this finding is demonstrated by the efficient synthesis of drug-like molecules with high step-economy. Preliminary mechanistic investigations reveal that this reaction underwent a sequentially migratory insertion to alkenes, metal migration process, and the aza-Michael addition to a quinone methide intermediate.
The identification of copy number variations in upland cotton using a high-density SNP array
Authors: Ma QingShan , Tao ZhiXin , Du Xiao , Moming Maimaiti , Ai XianTao 
Published: July 2022

Abstract
Background
Upland cotton (Gossypium hirsutum) is one crucial natural textile fiber and oilseed crop worldwide. Copy number variation (CNV) represents a form of genomic structural variation underlying phenotypic diversity. Understanding the genomic CNVs of Upland cotton is important for accelerating cotton breeding programs.Therefore,the present study generated a CNV regions (CNVRs) map in Upland cotton using the SNP array-based approach. The roles of CNVs identified were explored based on GO enrichment analysis of genes located in CNVRs.
Results
we used the Illumina Cotton SNP 70K BeadChip array for genome-wide detection of CNVs in 288 Upland cotton accessions. A total of 341 CNVRs were detected, representing approximately 2.0% of the entire cotton genome. Of these, 264 were gains, 55 were losses, 22 were events with gains and losses within the same region. The average length of CNVRs was 125.5kb and the median length was 80.4kb.The gene ontology (GO) enrichment analysis revealed that genes associated with metabolic process, catabolic process, multiple enzyme activities and transcription regulator activity were significantly overrepresented.
Conclusions
The findings that 90.3% of CNVRs harbored genes indicated the critical roles of CNVs in cotton. we identified two intriguing candidate biological process terms, polysaccharide metabolic process and catabolic process, related to cotton fiber development. Our research characterized the first landscape of CNVRs in cotton, providing an important resource for investigations of cotton genetic variability. It will facilitate further researches of molecular mechanisms underlying CNV-associated traits and improve our knowledge of genomic structure of cotton.
Increased Sample Size Proved More Insights for the Population Structure of Mediterranean Loggerhead Sea Turtles
Authors: Kaska Arzu , Koç Gizem , Sözbilen Dogan , Diryaq Salih , Glidan Ashraf , Elsowayeb Abd Alati , Saied Almokhtar , Margaritoulis Dimitris , Theodorou Panagiota , Rees Alan F. , Snape Robin , Broderick Annette , Godley Brendan , Beton Damla , Ozkan Meryem , Jr Imed , Ismail Manel Ben , Hmida Ahmed Ben , Badreddine Ali , Sacdanaku Enerit , Rae Vicky , Abdelwarith Mohamed Said , Naguib Nahla , Nakhla Lobna Ben , Limam Atef , Abderrahim Marwan , Sémelin Julien , Kaska Yakup 
Published: July 2022

Abstract
The loggerhead sea turtle (Caretta caretta), has the broadest distribution among sea turtle species in the Mediterranean and requires regional and international collaborations in addition to local efforts to better inform conservation actions. Molecular techniques are powerful tools to assess population dynamics at large scales, especially by determining the connectivity among different nesting and foraging sites, and genetic diversity. In this study, a large sample was collected synchronously in the nesting areas located in the north, south and east of the Mediterranean. Recently described nesting sites from Albania and other nesting sites represented by lower sample size were also included in order to fully assess the genetic composition of the region’s rookeries. Samples from 710 individuals were collected and the longer (815 bp) mtDNA D-loop fragment of these samples was amplified. We recorded 15 haplotypes, three of which were novel. In addition, our results show that some haplotypes, considered of Atlantic origin, have a wider dispersal in the Mediterranean than previously thought, albeit with low levels of representation. Our results, which also contribute to determining the likely origin of haplotypes that were previously known only from foraging sites, highlight the utility of broad-scale sampling, with increased sample number and longer mtDNA sequence to determine genetic diversity and connectivity. This study also demonstrates that it is important to continue to monitor the contribution of Atlantic origin haplotypes to the Mediterranean population, and the resident Mediterranean population, which is expected to expand its geographical range for reproduction with the effect of climate change and climate change in the long term. This work is important for, among other things, mixed stock analyses (MSA) that seek to localize the origin of stranded or accidentally caught sea turtles or those purposefully obtained from foraging sites to better understand the migratory distribution for conservation purposes.
Transgenerational herbivory effects on performance of clonal offspring of the invasive plant Alternanthera philoxeroides
Authors: Dong Bi-Cheng , Fu Qiu-Yue , Yu Cheng-Ling , Dong Ran , Shi Juan , Luo Fang-Li , Gao Jun-Qin , Yu Fei-Hai 
Published: July 2022

Abstract
Interactions between alien plants and the newly emerging enemies in introduced ranges may determine plant invasion success. However, little is known about whether herbivory-induced responses are transmitted across vegetative generations of plants, and whether epigenetic changes are involved during this process. In a greenhouse experiment, we examined the effects of current, parental and/or grand-parental herbivory by the generalist herbivore Spodoptera litura on the growth, physiology, and biomass allocation, and the status of DNA methylation of the first-, second- and/or third-generation clonal offspring of the invasive plant Alternanthera philoxeroides, which were derived from two types of root fragments with different branching orders (i.e., the primary- or secondary-root fragments of taproots). Parental herbivory promoted the growth of the second-generation plants sprouted from the secondary-root fragments, but had a neutral or negative effect on the growth of the second-generation plants from the primary-root fragments. The growth of the third-generation plants was significantly reduced by current herbivory, but not affected by grand-parental herbivory. The first-generation plants exhibited a higher level of DNA methylation when they were grazed by herbivores than when they were not, while neither the second-generation nor the third-generation plants showed the herbivory-mediated changes in DNA methylation. Overall, the herbivory-induced growth response within one generation may represent rapid acclimatization of A. philoxeroides to the unpredictable generalist herbivores in the introduced ranges. Herbivory-induced trans-generational effects may be transient for clonal offspring of A. philoxeroides, which can be influenced by the branching order of taproots, but be less characterized by DNA methylation.
Expression of DNA-repair genes and its relevance for DNA repair in peripheral immune cells of patients with posttraumatic stress disorder
Authors: Behnke Alexander , Mack Matthias , Fieres Judy , Christmann Markus , Bürkle Alexander , Moreno-Villanueva Maria , Kolassa Iris-Tatjana 
Published: July 2022

Abstract
Posttraumatic stress disorder (PTSD) involves elevated levels of cellular oxidative stress which jeopardizes the integrity of essential cell compartments. Previously, we demonstrated higher levels of DNA lesions in peripheral blood mononuclear cells (PBMCs) in PTSD. Retaining vital levels of DNA integrity requires cells to mobilize compensatory efforts in elevating their DNA-repair capacity. Accordingly, we hypothesized to find increased expression rates of the DNA-repair genes X-ray repair cross complementing 1 (XRCC1), poly (ADP-ribose) polymerase 1 (PARP1), and polymerase β (Polβ) in PBMCs of PTSD patients as compared to controls, leading to functionally relevant changes in DNA-repair kinetics. In a cohort of 14 refugees with PTSD and 15 without PTSD, we found significantly higher XRCC1 expression in PTSD patients than controls (U = 161.0, p = .009, Cohen’s r = .49), and positive correlations between the severity of PTSD symptoms and the expression of XRCC1 (rS = .57, p = .002) and PARP1 (rS = .43, p = .022). Higher XRCC1 (F = 2.39, p = .010, η²p = .10) and PARP1 (F = 2.15, p = .022, η²p = .09) expression accounted for slower repair of experimentally X-ray irradiation-induced DNA damage, highlighting the possible physiological relevance of altered DNA-repair gene expression in PTSD. Our study provides first evidence for a compensatory regulation of DNA-repair mechanisms in PTSD. We discuss the implications of increased DNA damage and altered DNA-repair mechanisms in immune senescence, premature aging, and increased physical morbidity in PTSD.