bluuebunny/crossref_metadata_2025_split huggingface dataset:
The regulation of autophagy by the MiR-199a-5p/p62 axis was a potential mechanism of small cell lung cancer cisplatin resistance
Authors: Li Tiezhi , Zhang Helin , Wang Zhichao , Gao Shaolin , Zhang Xu , Zhu Haiyong , Wang Na , Li Honglin 
Published: February 2022

Abstract
Background Autophagy has been found to be involved in the Multidrug resistance (MDR) of cancers, but whether it is associated with resistance of small cell lung cancer (SCLC) has not been studied. Here, we hypothesized that a potential autophagy-regulating miRNA, miR-199a-5p, regulated cisplatin-resistant SCLC. Methods We validated the MDR of H446/EP using CCK-8 and LDH. We tested the binding of miR-199a-5p to p62 using the Dual-Luciferase assay and validated the association of miR-199a-5p and p62 in SCLC samples. We overexpressed (OE) and knocked down (KD) miR-199a-5p in H446 and H446/EP and determined the expression of miR-199a-5p, autophagy-related proteins, and the formation of autophagolysosomes using QPCR, western blotting, and MDC staining respectively. These results were validated in an orthotopic H446 mouse model of SCLC. Results H446/EP was resistant to cisplatin, etoposide, paclitexal, epirubicin, irinotecan, and vinorelbine. Exposure of cisplatin at 5 μg/ml for 24 hours increased LC3II/LC3I, ATG5, p62, and the formation of autophagolysosomes in H446 cells, but not in H446/EP cells. The expression of miR-199a-5p was up-regulated in H446/EP compared to H446. MiR-199a-5p directly targeted the p62 gene. The expression of miR-199a-5p and p62 were correlated in SCLC samples. In H446 and H69PR, the OE of miR-199a-5p increased LC3II/LC3I, p62, and the formation of autophagolysosomes, but not ATG5, while the KD of miR-199a-5p decreased p62, but did not affect LC3II/LC3I, ATG5, and the formation of autophagolysosomes. In H446/EP, the OE of miR-199a-5p decreased p62 only. These results were generally consistent to results in the animal tumor samples. Conclusions The regulation of autophagy by the MiR-199a-5p/p62 axis was a potential mechanism of small cell lung cancer cisplatin resistance.
Improvement of the algorithm for diagnostics of postcholecystectomy syndrome taking into account functional and organic changes in the area of the large duodenal nipple
Authors: Veligotsky Nikolai , Aleksanian Karyna , Arutyunov Sergei , Gorbulitch Alexandr 
Published: February 2022

The aim. Improving the algorithm for diagnosing patients with postcholecystectomy syndrome (PCS), taking into account functional and organic changes in the major duodenal papilla (MDP).
Materials and methods. 208 patients with PCS were examined. Laparoscopic cholecystectomy (CE) was performed in 172 patients in history, open CE was performed in 36 patients. 47 patients had obstructive jaundice, 84 had signs of biliary hypertension without an increase in bilirubin, 77 had no signs of biliary hypertension and an increase in bilirubin.
The following instrumental research methods were used: abdominal organs ultrasound examination (AO USE), duodenoscopy, endoscopic retrograde cholangiopancreatography (ERCP), computed tomography (CT). In the main group, to study the morphofunctional changes in MDP, duodenoscopy with parietal impedancemetry, ultrasound of the hepatobiliary zone with a choleretic load were used.
Results. In the main group, 34 patients underwent ultrasound of the hepatobiliary zone and Vater's nipple area with a choleretic breakfast according to Boyden according to the method of Grigoriev P.Ya. in our modification, 21 patients had an expansion of the choledochus by 2 mm. At the same time, taking meverin led to a decrease in the diameter of the choledochus, which indicated functional changes in the MDP. In 13 patients, the expansion of the choledochus, observed when taking a choleretic breakfast, persisted with the use of antispasmodics, which indicated the presence of an organic pathology of MDP. Duodenoscopy with impedancemetry was performed in 41 patients: 20 patients had 760–820 Ohm, which indicated the presence of functional changes, 12 patients had inflammatory changes in the MDP and 703–760 Ohm were detected, 9 patients with cicatricial fibrous changes had indicators impedance measurement 640–703 Ohm. The use of ultrasound with a choleretic breakfast and duodenoscopy with parietal impedancemetry in patients of the main group made it possible to identify functional and organic (inflammatory, fibrous) changes in the MDP, which made it possible to develop a therapeutic algorithm using both conservative therapy and the use of various options for transpapillary endoscopic interventions.
Conclusions. Conducting an in-depth diagnosis of morpho-functional changes in MDP using parietal impedancemetry of MDP and ultrasound of the hepatobiliary zone with choleretic load allows us to identify both functional and morphological changes in MDP, which makes it possible to develop a therapeutic algorithm using drug therapy and transpapillary endoscopic techniques.
Identification and assessment of antimicrobial resistance bacteria in a hemodialysis water treatment system
Authors: Chaoui Laila , Chouati Taha , Zalegh Imane , Mhand Rajaa Ait , Mellouki Fouad , Rhallabi Naima 
Published: February 2022

AbstractThe water treatment process is a vital factor for hemodialysis (HD) patients. This study aimed to assess the degree of contamination of HD water by bacteria at the HD center of Mohammedia, Morocco, in addition to evaluating the antimicrobial resistance of isolated bacteria. Fifty-four water samples were taken, the appropriate cultures were used to isolate the pathogenic bacteria, which were identified biochemically and molecularly by 16S RNA sequencing. Their susceptibility to antimicrobial drugs was determined by the disk diffusion method. Approximately 5.5% of water samples were above the norm. The isolated bacteria that colonized the HD systems were mostly Gram-negative bacilli, such as Stenotrophomonas maltophilia, Pseudomonas spp., and Burkholderia cepacian. Results of the antibiotics test showed remarkable resistance levels. Among Pseudomonas spp. and S. maltophilia, 10 strains were classified as multidrug-resistant (MDR), and 4 as extensively drug-resistant (XDR). The diversity of bacterial strains isolated in the water used for HD treatments, and their worrying resistance levels pose a significant risk to patients. For these reasons, an urgent need for periodic microbiological monitoring of water after each treatment step must be applied, and the treatment process should also be optimized.
The Radicular Cyst in a Child: Case Report
Authors: Roci Bexhet , Dimitrovski Oliver , Ambarkova Vesna 
Published: February 2022

The most commonly occurring odontogenic cysts of the jaws are radicular cysts.
Causes of jaw cysts are different, numerous and depend on the type of cyst. The basic precondition for the occurrence of a cystic lesion is the previous presence of epithelial tissue at the site of future development of the cystic formation.
Radicular cysts are one of the most common odontogenic cysts and arise from epithelial so-called Malassez - these residues in the periodontal tissue, as a consequence of inflammation of the pulp. They can occur at any age, and are equally prevalent in both sexes. The aim of this paper is to present the clinical symptoms and treatment of a radicular cyst in a 12-year-old child.
The treatment of radicular cysts in most cases is surgical. The possibilities for such treatment and the choice of surgical method depend on the size of the cyst, its location, and the pathological formation.

The present article reported a case of a 12-year-old male with the chief complaint of pain on the right lower back tooth region. The radiographic examination revealed the presence of a well-defined radiolucency surrounded by a corticated border between mandibular central incisive. The case was managed by complete enucleation under local anesthesia without extraction of any teeth.
We chose enucleation (cystectomy) as a surgical procedure as the method of choice, because postoperative complications are the rarest, and the best healing is also provided. Аfter one month of surgery treatment, complete wound healing was recorded at the clinical control at the Department for oral surgery.
El tema gastronómico en el aula ELE
Authors: Arroyo Martínez Laura 
Published: February 2022

El presente artículo se incluye dentro del campo de investigación del análisis de materiales de la enseñanza-aprendizaje de Español como Lengua Extranjera (EELE). Consideramos esencial poner de relieve la importancia que estos trabajos tienen para alcanzar una adecuada práctica docente. En el marco empírico de este artículo se han seleccionado cuatro manuales muy populares y difundidos en la ELE en diversas instituciones y países. Dentro de los contenidos socioculturales que incluyen, se analiza particularmente cómo se abordan los contenidos gastronómicos, cómo se describen y cómo se ejercitan en el aula, según el modelo propuesto por Fernández López (2008).
Carências epistemológicas da temática fome no Currículo Paulista
Authors: Santos Paula Rodrigues de Oliveira , Silva Antonio Fernando Gouvêa da 
Published: February 2022

A fome na contemporaneidade não pode ser compreendida como resultado de um fenômeno natural e inevitável, mas sua gênese se dá em decisões sociais, fazendo parte de um projeto de sociedade pautada pela desigualdade. Portanto, envolve opções políticas para sua superação e, nesse sentido, uma educação dialógica e problematizadora poderá auxiliar na transformação dessas condições desumanizadoras. O currículo, como espaço de construção de significados, pode contribuir para a práxis de conscientização social no que tange a essa questão ou para perpetuar o problema. A educação é um ato político, portanto “o que ensinar” e “para quem” possui uma intencionalidade intrínseca, ligada à reprodução ou transformação da realidade social desigual, além disso não se pode desvincular a teoria da prática, visto que de nada adianta um discurso progressista visando a transformação social rumo à igualdade e justiça, se essa não estiver vinculada a prática. Para tanto, a educação popular freireana está orientada para posicionar-se ao lado dos oprimidos para sua conscientização e emancipação. Para tanto, buscou-se analisar o Currículo Paulista de acordo com três categorias freireanas: politicidade, historicidade e práxis autêntica, no sentido de contribuir para a abordagem da temática fome em uma perspectiva crítica. Utilizou-se a abordagem qualitativa pautada no materialismo histórico-dialético e análise do discurso de Fairclough (2001). Os resultados iniciais indicam que o Currículo Paulista enfatiza atender os interesses neoliberais e uma prática reprodutora e perpetuadora das desigualdades.
PESQUISA COMO MEIO DE FORMAÇÃO PERMANENTE
Authors: Guimaraes Ana Lídia Felippe , Sucupira da C. Lins Maria Judith 
Published: February 2022

O presente texto é o recorte da pesquisa realizada pelo Programa de Pós-Graduação em Educação – FE/UFRJ numa unidade escolar da Rede Municipal da Cidade do Rio de Janeiro. O sujeito da pesquisa para esse trabalho é uma professora de educação física. O objetivo é identificar a relevância da pesquisa empírica na formação e ação pedagógica no processo de inclusão da pessoa com deficiência. O objeto de estudo é o tema Ética na inclusão escolar. Para tal, a pesquisa-ação por meio da Escuta Sensível (1985) com análise de conteúdo à luz de Bardin (1977) foi a metodologia seguida. Um ponto crucial, no que tange aos resultados, foi a transformação da ação pedagógica da professora e o reconhecimento da transversalidade Ética frente ao processo de inclusão dos estudantes com deficiência.
COVID-19–Related Multisystem Inflammatory Syndrome in Children Presenting With New-Onset Type 1 Diabetes in Severe Ketoacidosis: A Case Series
Authors: Aly Hanan H. , Fouda Eman M. , Kotby Alyaa A. , Magdy Sondos M. , Rezk Ahmed R. , Nasef Marwa W.A. 
Published: February 2022

OBJECTIVE
To report and describe cases of children presenting with coronavirus disease 2019 (COVID-19)-related multisystem inflammatory syndrome in children (MIS-C) with new-onset type 1 diabetes mellitus (T1DM) in severe diabetic ketoacidosis (DKA).

RESEARCH DESIGN AND METHODS
This prospective observational study was conducted to characterize children with COVID-19–related MIS-C and new-onset T1DM who were in DKA. MIS-C was diagnosed if Centers for Disease Control and Prevention and World Health Organization criteria were fulfilled.

RESULTS
Six cases were identified. The patients were critically ill and in nonfluid responsive shock (combined hypovolemic and cardiogenic or distributive shock). All had cardiac involvement. One patient had a Kawasaki shock-like presentation. All needed aggressive treatment with careful monitoring of fluid balance (because of associated cardiac dysfunction), early institution of vasoactive/inotropic supports, and use of methylprednisolone and intravenous immunoglobulins. The latter are better administered after DKA resolution to avoid undue volume overload and fluid shifts while the patients are in DKA.

CONCLUSIONS
Awareness of MIS-C coexistence with DKA at T1DM onset is crucial for rapid proper management.
Preliminary studies of bioactive compounds and color in Mexican read wines from Baja California region.
Authors: Mendez-Trujillo Vianey , Gonzalez Mendoza Daniel 
Published: February 2022

The total phenolic compounds, total anthocyanin content and antioxidant properties of ten commercially wines based on different grape cultivar from Baja California, Mexico were analyzed. The results showed that the content of phenolic compounds of Vitis vinifera cv. Petit Verdot and Petit Syrah wines had the most highest values of total phenolic content (1188.26   and 1148.13 mg GAE/L, respectively), with respect the another wines. However, wines elaborated with three different cultivars (Cabernet Sauvignon/Petit Verdot/Merlot), and wine from cultivar Petit Verdot presented the highest total flavonoids content (161.06   mg QE/L and 182.64 mg QE/L, respectively) and total anthocyanin content (286.38 mg ME/L and 2548.56 mg ME/L, respectively). In contrast only wine from three grape cultivars (Cabernet Sauvignon/Petit Verdot/Merlot), and wine from cultivar Gamay, exhibits the highest antioxidant activity among tested wines. Finally, these results suggest that wine elaborated with different cultivars (Cabernet Sauvignon/Petit Verdot/Merlot), is more beneficial to health than others red wines in terms of its higher antioxidant activity, total anthocyanin content and total flavonoid content
Indirect Tax Administration Efficiency of the Royal Malaysian Customs Department
Authors: Mutahar Nur Azhari , Abd Hamid Nadiah 
Published: February 2022

The method used to measure indirect tax administration efficiency is crucial; hence, this study will measure indirect tax administration efficiency using the cost-revenue ratio. Malaysia had experienced several changes related to its indirect tax system where the shift of Malaysia’s indirect tax regime is also linked to the efficiency of the indirect tax administration. The lack of empirical research on this matter urges the current study to determine the trend of indirect tax administration efficiency level under the RMCD. In this study, secondary data were obtained from RMCD Annual Report, Ministry of Finance’s Federal Government Estimated Revenue Report, Budget Report, and Treasury Annual Report to examine the trend of RMCD’s tax administration efficiency level for 7 years (2013-2019). Both administration costs and total tax revenue collection were found to affect the efficiency level of the Royal Malaysian Customs Department (RMCD); however, there is no strong evidence to claim that administration costs indeed influence the total indirect tax revenue collection and vice-versa. Research findings and initial inputs from the RMCD Annual Reports are analysed to discuss and provide recommendations for future research works. The research findings provide important information for the RMCD and policymakers in improvising indirect tax administration.
Issues and Challenges of SME’s Food Entrepreneurs in Dungun, Terengganu
Authors: Zakaria Zainuddin , Mohd Abd Majid Hayati Adilin , Ngali Norzaidah , Kamaludin Mohd Ariff 
Published: February 2022

This project is based on the concern of the university on the issue of small and medium entrepreneurs (SMEs) in the food processing industry. Among the issues faced by the SMEs are the lack of competitiveness and marketing effectiveness. They still have many shortcomings in the industry, especially in food hygiene, packaging, labelling as well as marketing. We are targeting at least 60% of small and medium-sized entrepreneurs, especially those involved in the processing industry, food packaging and food stall management. Previous studies have found that SMEs have constraints in proper handling, processing and knowledge that coincide with local and international standards. In addition, they are facing problems in acquiring HALAL and MESTI certification. A qualitative research approach was adopted with an exploratory, inductive research. The sampling method used in this research is purposive sampling since only respondents with the selected criteria will be considered as a respondent in the study. A minimum 20 entrepreneurs were selected based on the entrepreneurs list provided by Majlis Amanah Rakyat (MARA) of Dungun and Lembaga Terengganu Tengah (KETENGAH). They are involved in the food industry such as frozen food, dry chips, cakes, fruit juice etc. We believe this project can help them address the problems and challenges mentioned earlier as the proposed project is a holistic project that will help them to become competitive and quality-conscious entrepreneurs. This project will also help entrepreneurs and food stall operators to improve the quality of their food products. Without this project, we feel that the entrepreneurs will continue facing difficulty in terms of their ability to produce high-quality food products and penetrating new and larger local markets.
Serum cobalamin concentrations in dogs infected with canine parvoviral enteritis
Authors: Engelbrecht Monique , Botha Willem Jacobus , Pazzi Paolo , McClure Vanessa , Hooijberg Emma 
Published: February 2022

Abstract

OBJECTIVE
To compare the serum cobalamin concentrations in canine parvovirus (CPV)–infected dogs with those of healthy control dogs.

Animals
45 dogs with CPV enteritis and 17 healthy age-matched control dogs.

Procedures
Infection was confirmed by visualization of CPV-2 through fecal electron microscopy. All dogs received supportive care. Serum samples taken at admission were used to determine cobalamin, C-reactive protein, and albumin concentrations.

Results
Serum cobalamin concentrations were significantly lower in the CPV-infected group (median [interquartile range], 173 pmol/L [< 111 to 722 pmol/L]) than in healthy control dogs (379 pmol/L [193 to > 738 pmol/L). There was no association between cobalamin concentration and C-reactive protein or albumin concentration.

Clinical Relevance
While hypocobalaminemia was common in CPV-infected dogs, the clinical relevance of this finding remains to be determined. Studies assessing markers of cellular cobalamin deficiency in dogs with CPV infection appear warranted.
Public Service Media in the Age of Social Networks
Authors: Glózer Rita 
Published: February 2022

Abstract
Digital transformation and the expansion of social networks have essentially modified journalistic practice, facing challenges concerning its essential operation and boundaries. The traditional business models have collapsed, young people’s media consumption habits have changed, and more non-professional journalists participate in news production. Social media platforms have an immense effect on journalistic work, especially journalists’ professional and ethical standards. Perhaps traditional public service media are the ones that stand the farthest away from participatory practices and platform logic, characterizing today’s media environment. What are the public media services’ opportunities to fulfil their commitment of authentically and objectively informing citizens, including young people, about public affairs? This study aims to picture the boundary work of journalism currently underway by reviewing the phenomena emerging on the border of journalism and social media. Additionally, the study attempts to answer what a successful social media strategy looks like in public service media by presenting the social media activities of “tagesschau”, the newsmagazine of ARD, a German public-service channel.
New Digital Cinema: How Platforms Are Changing the Audiovisual Industry
Authors: Capalbi Antonella , Fabbri Tommaso , Iervese Vittorio 
Published: February 2022

Abstract
Digitalization has been changing the methods of production, distribution, and use of written and audiovisual products, according to a process of “platforming”. Most audiovisual content is available via streaming platforms, through a lot of devices (PCs, tablets, smartphones, smart TVs, and videogame consoles), which have empowered the process of media convergence. Based on a theoretical framework related to the impact of the platforms on the ongoing process, we intend to analyse how platforms are changing the identity of the audiovisual industry and how they represent themselves online. In order to analyse the ongoing process, we intend to examine some platforms that could be considered as best practices in the audiovisual field: Netflix, MUBI, and Festival Scope. On the one hand, using these platforms as case studies will enable us to highlight some beneficial aspects of the ongoing process of digitalization and platforming: holding many gazes and points of view; catching market niches; building a peer-to-peer network. On the other hand, we intend to emphasize some risks connected to the intermediation of platforms in the audiovisual field in terms of economic, cultural, and social effects.
Beyond Netiquette: Digital Citizenship as Participation
Authors: Bakó Rozália Klára 
Published: February 2022

Abstract
Digital citizenship research has been on the agenda of scholars and practitioners since 1999 and has been trending since 2015. A plethora of approaches and definitions have been arising, with two major directions: a theoretical-methodological and a practical-educative. The present critical literature review is aimed at advocating for a more civic approach to the issue of digital citizenship and at presenting arguments in favour of a research agenda focused on the participatory components of digital life. Our desk research operated with both original studies and meta-analyses related to the concept of digital citizenship. While being technically savvy and well-behaved online is a key requirement for today’s netizen, becoming a citizen in virtual spaces requires more. Beyond netiquette, civic participation online is becoming a core competence.
Online Identities in Politics. Technological and Content-Based Approach
Authors: Szűts Zoltán 
Published: February 2022

Abstract
In this study, we examine a particular form of organizational identity, the issue of political organizational identity. We have chosen a technology and content-based approach and are looking for the answer to how technology influences identity formation, what role social media plays in shaping online political communities, and what characterizes a definable and strong online political identity. The impact of new communication technologies and media platforms has now reached all levels of society. It has influenced many aspects of everyday life, transformed politics, the economy, and culture, and has an impact on institutional identities as well. To identify the forces shaping institutional identity, we examine the role of digitalization, network technologies, and algorithms and the presence of social media and, finally, bring the Occupy Wall Street movement as an example of reshaping online identities.