bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integration of Transplanted Choroidal Plexus Epithelial Cells with Oxygen Glucose Deprived Organotypic Spinal Cord Slices
Authors: Liu Jing-Jie , Ding Xiao-Yan , Xiang Li , Huang Sheng-Li 
Published: March 2022

Abstract
This study was undertaken to evaluate the integration of transplanted choroidal plexus epithelial cells with oxygen glucose deprived (OGD) organotypic spinal cord slices. Organotypic spinal cord slices were normally cultured for 14 days, then were OGD for 60 min. They were divided into transplantation group and non-transplantation group. The choroidal plexus epithelial cells, which were cultured for 6–7 days, were labeled by 1,1’-dioctadecyl-3,3,3’,3’-tetramethyl-indocarbocyanineperchlorate(CM-Dil), and were identified by transthyretin (TTR) in immunocytochemistry. They were adjusted to the density of 0.5–1×107/ml. 2µl cells suspension were transplanted into OGD spinal cord slices in transplantation group. Subsequently, the slices were normally cultured for 3 days (NT 3 d group), 7 days (NT 7 d group) and 14 days (NT 14 d group) respectively. Accordingly, in non-transplantation group, 2µl basal medium was dripped to the OGD organotypic spinal cord slices, then the slices were normally cultured for 3 days (N 3 d group), 7 days (N 7 d group) and 14 days (N 14 d group). After 3 days and 7 days of transplantation, the level of LDH in the culture medium was tested by Elisa, and the ratio of caspase 3 active/ caspase 3 was tested by western blotting. After 14 days of transplantation, the differentiations into neurons and astrocytes, and the synapses were identified by immunofluorescence histochemistry. At the same time, the ratios of cell differentiations and synapses in new system, and the changes of MAPK signaling pathway were tested by western blotting. Our result shows that the choroidal plexus epithelial cells were well labeled by CM-Dil and were immune-stained by TTR in immunocytochemistry. The transplantation cells could differentiate into neurons and astrocytes, and there were some synaptophysin positive vesicles between transplantation cells and OGD organotypic spinal cord slices. The level of LDH and the ratio of caspase 3 active/ caspase 3 in the NT 7 d group was significantly lower than which in the N 7 d group (P < 0.05), but there was of no significance between NT 3 d group and N 3 d group. The levels of GFAP, TUB-III and synaptophysin in the NT 14 d group were significantly higher than which in the N 14 d group (P < 0.05). The ratios of p-JNK/JNK and p-P38/P38 in the NT 14 d group were significantly lower than which in the N 14 d group (P < 0.05). But the ratios of p-ERK/ERK in the NT 14 d group were significantly higher than which in the N 14 d group (P < 0.05). The transplanted choroidal plexus epithelial cells can integrate with OGD organotypic spinal cord slices into a new system.
Association between change in heart rate over years and life span: Paris Prospective 1, Whitehall 1, and Framingham studies
Authors: Gaye Bamba , Valentin Eugenie , Xanthakis Vanessa , Perier Marie-Cecile , Celermajer David , Shipley Martin , Marijon Eloi , Song Rebecca , Empana Jean-Philippe , Ramachandran Vasan , Jouven Xavier 
Published: March 2022

Abstract
We examined the association between resting heart rate and life span using linear regression in the Paris Prospective Study I, the Whitehall I Study, and the Framingham Heart Study. We used Cox proportional hazards regression to relate change in heart rate over years to mortality risk. We confirmed the inverse relation between resting heart rate and life span, observed a shift to the right (an increase) in life span over the past 250 years in humans compared to other mammals, and observed that higher resting heart rate was associated with higher risk of mortality after adjustment for standard risk factors.
Tilapia Invasion Impacts Trophic Position And Resource Use Of Commercially Harvested Piscivorous Fish In The Large Subtropical Pearl River
Authors: Shuai Fangmin , Li Jie , Lek Sovan 
Published: March 2022

Abstract
Species invasions pose a serious threat to native biodiversity and ecosystems. However, quantifying the impacts of invasive species has proven problematic. In this study, we quantified the trophic changes in freshwater food webs invaded by tilapia, using an extensive stable isotope dataset to compare uninvaded and invaded rivers downstream of the Pearl River, China. The trophic position of the widely distributed and locally economically important piscivorous culter fish (Erythroculter recurviceps), mandarin fish (Siniperca kneri), and catfish (Pelteobagrus fulvidraco and Pelteobagrus vachelli) decreased significantly in the invaded river compared to the uninvaded river. Our analysis indicated that the decrease in tropic position of these piscivorous fishes reflected a major reduction in the proportion of prey fish biomass as a result of tilapia invasion. Stable isotope mixing models (SIMMs) indicated that small fish in the diet of culter fish from the reference river (32.7% small fish, 17% zooplankton) were replaced by lower trophic level zooplankton prey in the invaded river (35.7% zooplankton, 25.4% small fish), due to the presence of tilapia. Small fish in the diet of mandarin fish in the reference river (46.2% small fish, 10.5% aquatic insects) was replaced by lower trophic level aquatic insect prey in the invaded river (20.3% aquatic insects, 29.9% small fish). Fish eggs in the diet of catfish from the reference river (25.0% fish eggs, 25.2% aquatic insects) were replaced by aquatic insects at a lower trophic level in the invaded river (43.5% aquatic insects, 4.8% fish eggs). The results of this study contributed to a growing body of evidence, showing that tilapia could modify trophic interactions, which had severe consequences in invaded ecosystems.
Performances of Mini Parasep SF stool concentrator kit , Kato-Katz and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis among residents in an endemic community in northeast Thailand
Authors: Kopolrat Kulthida Y. , Singthong Seri , Khuntikeo Narong , Loilome Watcharin , Worasith Chanika , Homwong Chutima , Wangboon Chompunoot , Yasaka Patiwat , Eamudomkarn Chatanun , Pitaksakulrat Opal , Tonkhamhak Krisnakorn , Paeyo Arunee , Crellen Thomas , Sithithaworn Jiraporn , Sithithaworn Paiboon 
Published: March 2022

Abstract
Background
Control and elimination of the liver fluke (Opisthorchis viverrini) is a primary preventive strategy against cholangiocarcinoma in Southeast Asia. A sensitive parasitological diagnostic method is required to facilitate such a surveillance and control program. In this study, we evaluated the performance of Mini Parasep SF stool concentrator kit (stool kit) in comparison with Kato-Katz (KK) and the quantitative formalin-ethyl acetate concentration technique (FECT) for detection of O. viverrini and coexisted parasitic infections.
Methods
A cross-sectional survey of parasitic infection of residents age <15 years old in a community in Kalasin province, northeast Thailand was conducted in 2018. Fecal samples were collected and screened by KK method and a subset of samples was examined by the stool kit and FECT methods. The results were analyzed for prevalence of parasitic infections and diagnostic performances of methods for qualitative and quantitative detection of helminthiasis.
Results
The initial survey of parasitic infection determined by the KK method (n=567) showed the prevalence of O. viverrini was 32.63%, followed by Taenia 2.65%, echinostomes 1.76%, hookworms 1.41%, Trichuris trichiura 0.53%, and Strongyloides stercoralis 0.53%. Within a subset of samples tested with multiple diagnostics (n=150), the detection rates of O. viverrini by the stool kit, FECT, and KK methods were 27.3%, 30.7%, and 28.7%, respectively. The diagnostic sensitivity for opisthorchiasis were similar for FECT (75.4%) and KK (70.5%) and was lower for the stool kit (67.2%, p<0.05). For other parasitic infections, FECT and stool kit performed better than KK methods particularly in detecting minute intestinal fluke (MIF), S. stercoralis, and coinfections. The intensity of O. viverrini infection (fecal egg counts) by the stool kit had significant positive correlations with KK as well as FECT (p<0.05).
Conclusions
As the stool kit is simple to use and showed a comparable performance to FECT, it may serve as an alternative method of fecal examination for screening of helminthiasis including opisthorchiasis.
Structural Operational Integrity - The Comprehensive Quality Characteristic of Structure
Authors: He Yuting 
Published: March 2022

Abstract
Carbon reduction has been closely bound up with economy and social development. So, a higher demand for structural integrity is brought to meet the different requirements of equipment, ma-chines and infrastructures. For structures, one of their general quality characteristics such as reliability, safety, maintainability and so on, only reflects a certain aspect of quality characteristics. In this paper, After discussing equipment integrity which can be classified into equipment manufacture integrity, equipment storage integrity and equipment operational integrity along the life cycle time, from the perspective of performing and completing the functions and missions of structures, the putting forward process of structural operational integrity(SOI) is discussed, and the basic concept of structural operational integrity is shown: it is the attribute which exists when a structure is sound and unimpaired in full service or whole operational processes, with the statement that structural operational integrity can be used to describe the comprehensive quality characteristics of structures in their whole service or operational processes presented. Then, structural operational integrity is categorized into structural static operational integrity(SSOI) and structural dynamic operational integrity(SDOI), While definitions of them are given as follows: structural static operational integrity is the status which exists when a structure is sound and unimpaired in service or operational process, and structural dynamic operational integrity is the ability which exists when a structure is sound and unimpaired while providing the desired levels of structural durability, supportability, safety, performance, survivability and recoverability during service period or in operational processes. Furthermore, the characterizations of structural static operational integrity(SSOI) and structural dynamic operational integrity(SDOI) are presented，while the measurement methods of durability, supportability, safety, performance, survivability and recoverability of structures are discussed. Finally, it states that PHM technologies can improve structural operational integrity, and the optimum design method of structures based on structural operational integrity is described with an aircraft wing structure design as an example. With the employment of structural operational integrity, the structural design idea will be developed. It can help us get lighter, low life cycle cost, durable, fast recovery and more reliable structures. And structural operational integrity can be applied to other engineering structures such as building structures, bridge structures, infrastructure bodies, etc.
Development of a Quantitative Methylation-Specific Droplet Digital PCR Assay for Detecting Dickkopf-related Protein 3
Authors: ARAKI Kenji , KUROSAWA AI , KUMON HIROMI 
Published: March 2022

Abstract
Objective: The detection and monitoring of DNA methylation status in circulating tumor cell DNA (ctDNA) provides critical insights into cancer diagnosis and progression. The methylation status of the Dickkopf-related protein 3 (DKK3) promoter region is correlated with the metastasis and recurrence of multiple cancers. Thus, detecting the methylation status via non-invasive methods is essential for the diagnosis and prognosis of cancers. Using a droplet digital polymerase chain reaction approach, we have developed a highly sensitive and quantitative measurement of methylated and unmethylated DKK3 derived from circulating cell-free DNA (ccfDNA). Results: We confirmed the specificity of droplet digital methylation specific polymerase chain reaction (ddMSP). We selected the optimal bisulfite conversion method using commercially available kits. We validated the ddMSP analysis system by analyzing the methylation status of genomic DNA extracted from cultured mesothelioma cells and mesothelial cells. Our system quantified approximately 30 copies of cell-free DNA per 4 mL, which is sufficient for detecting ctDNA. Finally, we quantified methylated and unmethylated DKK3 copies in ccfDNA from 21 patients with malignant mesothelioma.
Urea Amendment Alleviated Morphological and Physiological Damages and Yield Loss of Winter Wheat Subjected to Cold Stress at Jointing Stage
Authors: Li Chunyan , Yang Jing , Zhu Min , Ding Jinfeng , Zhu Xinkai , Zhou Guisheng , Guo Wenshan 
Published: March 2022

Abstract
Cold stress in spring is an abiotic stress limiting the growth and productivity of winter wheat. A controlled pot experiment was done to explore the possibility of applying urea to alleviate the low temperature (3°C/−4°C, day/night)damage to wheat cultivar Yangmai 16 at jointing stage. Urea at different rates was applied at the 5th day after cold stress. Cold stress decreased grain yield and plant height. Compared with the unstressed control, the content of soluble sugar, proline, zeatin riboside (ZR), and abscisic acid (ABA) in the leaves was increased under longer cold stress. These above parameters in the stressed treatments without urea amendment were higher than those with urea amendment on the 10th day. The change in GA3 concentration was opposite to the concentrations of ABA and ZR. The decline in the concentrations of osmotic adjustment substances, balanced hormone concentrations, and increased grain number per ear and ear number were the main reasons for the increased grain yield after urea application. Remedial effects was enhanced with the higher urea remedial level under the same cold stress duration. Our study suggested that suitable urea remedial rates are recommended based on the freezing index in wheat to alleviate the impacts of low temperature on wheat production at jointing stage.
The four-drug fixed-dose combination reduced the loss to follow up from TB treatment in Brazil: additional evidence comparing two prospective cohorts at the same health centers
Authors: Oliveira Marina de Aguiar , Bento Joseane de Lima , Costa Ronaldo Rodrigues , Leite Isabel Cristina Gonçalves , Silva Marcio Roberto 
Published: March 2022

Abstract
Background: Loss to follow up (LTFU) is an obstacle to improving tuberculosis (TB) indicators. The aim of this study was to evaluate the incidence of and risk factors for LTFU from TB treatment by comparison of two prospective cohorts enrolled at the same health centers in southern Brazil: patients who used RH-FDC + Z regimen (2007-2010) and 4-FDC regimen (2018-2019). Methods: Predictors of LTFU were investigated using univariate and multivariate multilevel Cox regression models. Results: Of the 296 cases included, 247 (83.5%) and 49 (16.5%) had cure and LTFU as outcomes, respectively. Patients treated in first period (RH-FDC + Z regimen) and those with substance abuse (drug addicts, alcoholism, and smokers) had a higher risk for LTFU from TB treatment (p < 0.05). Accordingly, the cases treated with RH-FDC + Z regimen showed a HR of 2.25 (95% CI: 1.03 - 4.94, p = 0.042). Conclusions: The main result of this research was the significant reduction in the LTFU from TB treatment after the consolidation of the 4-FDC in Brazil, strengthening the premise that the presentation of drugs in this way improves treatment adherence. Also, substance abuse should be investigated more carefully in TB patients and systematically recorded as risk factors for LTFU, to contribute to better outcomes. Although the TB treatment 4-FDC regimen analyzed is already in use, these new regimens should be implemented with simultaneous pharmacovigilance studies and pragmatic cohort or trial designs to simulate real-world clinical practice, as done by this study.
Down-regulated Expression Of CDK5RAP3 and UFM1 Suggests A Bad Prognosis In Gastric Cancer Patients Running title: CDK5RAP3 and UFM1 in gastric cancer
Authors: Lin Mi , Lian Ning-Zi , Cao Long-Long , Yoon Changhwan , Huang Chang-Ming , Zheng Chao-Hui , Li Ping , Xie Jian-Wei , Wang Jia-Bin , Lu Jun , Chen Qi-Yue , Li Ya-Han , Peng Zhu-Huai , Zhang Xiao-Yu , Mei Yi-Xian , Lin Jian-Xian 
Published: March 2022

Abstract
Purpose: The relationship between the CDK5RAP3 and UFM1 expression and the prolonged outcomes of patients who underwent gastric cancer (GC) surgery was investigated. Methods: Single-sample gene set enrichment analysis (ssGSEA), unsupervised clustering and other methods were used to verify the relationship between CDK5RAP3 and UFM1 in GC through public databases. Additionally, CDK5RAP3 and UFM1 expression in cancerous and paracancerous tissues of GC was analysed in the context of patient prognosis. Results: CDK5RAP3 and UFM1 expression was downregulated synchronously, the interaction was existed between the two proteins, and UFM1 and CDK5RAP3 expression was found to be inversely associated to AKT pathway activation. Prognostic analysis showed that the prognosis is poorer for low CDK5RAP3 and UFM1 patients, than for high CDK5RAP3 and/or UFM1 (p<0.001) patients, and this expression pattern was an independent predictor for overall survival of GC. Coexpression of CDK5RAP3 and UFM1 combined with TNM staging can improve the accuracy of prognosis prediction for patients (p <0.001). Conclusions: It is confirmed in our findings that a combination of CDK5RAP3 and UFM1 can produce a more precise prediction model for GC patients' survival.
Alien Advantage in Endozoochorous Seed Dispersal of Aquatic Plants
Authors: Lovas-Kiss Ádám , Navarro Maria José , Vincze Orsolya , Löki Viktor , Urgyán Renáta , Pallér Falicia Kapusi , Leeuwen Casper van , Green Andy J. , Lukács Balázs András 
Published: March 2022

Abstract
The expansion of alien plant species is of global concern, yet our understanding of their dispersal mechanisms is limited, especially regarding the role of migratory birds. We hypothesize that invasiveness of alien wetland plants might depend on specific adaptations that ensure high seed survival in the digestive tract of waterfowl, ensuring their efficient spread across sites. In a controlled feeding experiment, we compared the endozoochorous dispersal ability of twelve congeneric alien and native wetland plant species, with a particular focus on seed passage rate, gut retention time, germinability, and time to germination. Germination dynamics were also compared to those of control seeds. Finally, we test how seed traits explain the observed differences. Gut passage rate was higher and time to germination faster, but retention time and germinability were lower in alien than in native species. Controlling for seed traits did not alter these conclusions. Among control seeds, aliens germinated faster and their germinability was higher than in natives. Seed traits explained differences in germinability but not time to germination in control seeds. Our results indicate that endozoochory provides competitive advantage to alien plants by ensuring higher propagule pressure in new environments, potentially ensuring their quick spread.
Fault Diagnosis Based on Compressed Sensing of Multisource Data
Authors: You Xianglong , Deng Zhongwei , Zhang Kai , Li Jiacheng , Yuan Hang 
Published: March 2022

Abstract
With the development of the internet of things (IoT) and the application of 5G communication, fault diagnosis of massive multisource sensor data becomes more and more important. In this study, a compressed multisource sensor data-based fault diagnosis scheme is proposed, and its advantages include high data compression and fusion efficiency, low computational cost, and a fast online training sample updating rate. The method includes reference matrix construction, reference matrix compression and fusion, sparse vectors calculation, testing sample reconstruction, and quality evaluation. First, a reference matrix is constructed with labeled multisource sensor data, and each column in the matrix is composed of data samples collected from different sources. Then, the reference matrix is compressed using a measurement matrix, meanwhile, the multisource data samples are fused based on weighted summation during the compression. Later, sparse representation based on batch matching pursuit algorithm is conducted, in this step, the compressed testing sample is represented by the compressed reference matrix, and the output of the sparse representation is a sparse vector. After that, elements in the sparse vector corresponding to different patterns are retained exclusively while other elements are set to zero, respectively, and estimated testing samples are reconstructed with the compressed reference matrix and the processed sparse vector. Finally, based on reconstruction quality evaluation, the pattern of the testing sample is determined. Two cases are employed to validate the effectiveness of the proposed approach, including landfill gas power generator maintenance pattern recognition and multiple redundancy aileron actuator fault diagnosis, and the detection accuracy is 96.13% and 96.67%, respectively.
The Adoption Of Industrial Robots And Pollution Abatement In China
Authors: Wang Ying , Feng Jiahao 
Published: March 2022

Abstract
This paper explores the effects of the adoption of industrial robots on pollution abatement for Chinese firms. We find that industrial robots can significantly lower SO2 emission intensity. Panel quantile regression shows that industrial robots have greater effects on reducing SO2 emission intensity at low quantiles. Heterogeneity test shows that the reduction of SO2 emission intensity in labor- and technology-intensive industries benefits more from industrial robots. Our findings indicate that industrial robots play important roles in pollution abatement while more efficient policies should be implemented to promote the combination of intellectualization and green development for heavily polluting industries.JEL Classification: O33, Q53, Q58
Genetic Basis Underlying Tiller Angle in Rice (Oryza sativa L.) by Genome-wide Association Study
Authors: Bai Shaoxing , Hong Jun , Su Su , Li Zhikang , Wang Wensheng , Shi Jianxin , Liang Wanqi , Zhang Dabing 
Published: March 2022

Abstract
Rice tiller angle is a key agronomic trait determining rice grain yield. Several quantitative trait loci (QTLs) affecting rice tiller angle have been mapped in the past decades. little is known about the genetic base of tiller angle in rice, because rice tiller angle is a complex polygenic trait. In this study, we performed genome-wide association study (GWAS) on tiller angle in rice using a population of 164 japonica varieties derived from the 3K Rice Genomes Project (3K RGP). We detected a total of 18 QTLs using 1,135,519 single-nucleotide polymorphisms (SNP) based on three GWAS models (GLM, FastLMM and FarmCPU). Among them, two identified QTLs, qTA8.3 and qTA8.4, overlapped with PAY1 and TIG1, respectively, and additional 16 QTLs were identified for the first time. Combined with haplotype and expression analyses, we further revealed that PAY1 harbors one non-synonymous variation at its coding region, likely leading to variable tiller angle in the population, and that nature variations in the promoter of TIG1 significantly affect its expression, closely correlating with tiller angle phenotypes observed. Similarly, using qRT-PCR and haplotype analysis, we identified 1 and 7 candidate genes in qTA6.1 and qTA8.1 that were commonly detected by two GWAS models, respectively. In addition, we identified 3 more candidate genes in the remaining 14 novel QTLs after filtering by transcriptome analysis and qRT-PCR. In summary, this study provides new insights into the genetic architecture of rice tiller angle and candidate genes for rice breeding.