bluuebunny/crossref_metadata_2025_split huggingface dataset:
The clinical impact of IKZF1 mutation in acute myeloid leukemia
Authors: Zhang Xiang , Huang Aijie , Liu Lixia , Qin Jiayue , Wang Chengcheng , Yang Min , LOU Yinjun , Wang Lei , Ni Xiong , Hu Xiaoxia , Tang Gu-sheng , Zhang Mengmeng , Cao Shanbo , Mao Liping , Qian Jiejing , Xu Weilai , wei Ju-Ying , Xu Gaixiang , Meng Haitao , Mai Wenyuan , Yang Chunmei , Zhu Hong-Hu , Tong Hong , Yang Jianmin , Yu Wenjuan , Wang Jianmin , Jin Jie 
Published: May 2022

Abstract
The genetic heterogeneity generates great challenges in the understanding and treating acute myeloid leukemia (AML), while the knowledge about IKZF1 mutation in AML was extremely limited. Previously, we described the distribution pattern of IKZF1 mutation in AML, but its clinical impact remained undefined due to limited cases. Herein, we devoted to answer this question in one relatively large cohort. Among 530 newly diagnosed AML patients, we identified 28 IKZF1 mutations in 22 patients (22/530, 4.15%). IKZF1-mutated patients presented one young median morbid age (P=0.027) and the high frequency of mixed lineage leukemia involvement (P=0.012). In AML, IKZF1 mutation showed significant co-occurrences with CEBPA (P<0.001), SF3B1 (P=0.001), or CSF3R (P=0.008) mutation. Though IKZF1-mutated AML was more classified into the intermediate-risky group (P=0.005), it showed one inferior complete remission rate (P=0.041). Furthermore, AML with high variant allele frequency of IKZF1 mutation showed one relatively shorter overall survival duration (P=0.047), and it was one independent factor for the increased risk of death (hazard ratio, 3.14; 95% CI, 1.27-7.75; P=0.013). In subgroup analysis, our results showed that IKZF1 mutation conferred one poor therapeutic response and prognosis for SF3B1-mutated AML (P=0.002). Therefore, IKZF1 mutation exhibited one unique distribution mode and essential prognostic role in AML.
Tislelizumab in combination with gemcitabine plus cisplatin chemotherapy as first-line adjuvant treatment for locally advanced or metastatic bladder cancer: a retrospective study
Authors: Ren Xiang , Tian Yiqun , Wang Zhixian , Wang Jing , Li Xing , Yin Yisheng , Chen Ruibao , Zhan Ying , Zeng Xiaoyong 
Published: May 2022

Abstract
Background: Combining immune checkpoint inhibitors (ICIs) with chemotherapy can synergistically improve antitumor activity and are generally well tolerated. Recently, the efficacy and safety of combination therapy has been demonstrated for many cancers, including urothelial carcinomas. The aim of this retrospective pilot study was to evaluate the efficacy and safety of tislelizumab plus chemotherapy as first-line adjuvant treatment for locally advanced or metastatic bladder cancer.Methods: We conducted a retrospective analysis of 31 patients with locally advanced or metastatic bladder cancer from December 2020 to January 2022 with an Eastern Cooperative Oncology Group performance status (ECOG) of 0/1. Of the 31 patients, 14 patients received tislelizumab (200 mg i.v. every 3 weeks, Q3W) plus 21-day cycles of chemotherapy (gemcitabine, 1000 mg/m² i.v. on days 1 and 8 of each cycle + cisplatin, 70 mg/m² i.v. on day 2 of each cycle) (TGC) treatment and 17 patients received gemcitabine plus cisplatin chemotherapy (GC) treatment. All patients treated with bladder cytoreductive surgery and were treated for four 21-day cycles until disease progression or intolerable treatment-related adverse events (TRAEs). The objective progression-free survival (PFS), overall survival (OS), overall response rate (ORR), disease control rate (DCR), clinical benefit rate (CBR) and TRAEs were recorded and reviewed. Results: As of the cut-off date (March 25, 2022), PFS, OS, ORR, DCR, CBR and TRAEs were evaluated in 14 patients receiving combination therapy and 17 patients in the chemotherapy alone group. The median PFS was 36.0 [95% confidence interval (CI), 33.1-38.9] weeks in the TGC group and 29.0 (95% CI, 25.4-32.6) weeks in the GC group [hazard ratio (HR), 0.15 (95% CI, 0.04-0.55)]. In the GC group, the median OS was 48.0 (95% CI, 39.7-56.3) weeks; the median OS was not yet mature for the TGC group [HR, 0.26 (95% CI, 0.07-0.94)]. Treatment with TGC resulted in improved DCR (TGC, 71.4%; GC, 65.0%) and CBR (TGC, 64.3%; GC, 52.9%) compared with GC. However, although higher incidences of grade ≥3 TRAEs were observed with TGC compared with GC (35.7% versus 23.5%), the difference was not statistically significant (p = 0.47).Conclusion: This study suggested that TGC provided survivors of locally advanced or metastatic bladder cancer with encouraging antitumor activity and was generally well tolerated.
Impact of PD-L1 gene polymorphism and its interaction with cooking with solid fuel exposure upon susceptibility to tuberculosis
Authors: Tang Kun , Zhong Hua , Wang Qiaozhi , Li Zihao , Wu Chunli , An Rongjing , Lin Ying , Wang Jing , Wang Mian , Tan Hongzhuan , Chen Lizhang , Chen Mengshi 
Published: May 2022

Abstract
Given that PD-L1 is a crucial immune checkpoint in regulating T cell responses, the aim of this study was to explore the impact of PD-L1 gene polymorphism and its interaction with cooking with solid fuel on susceptibility to tuberculosis (TB) in Chinese Han populations. A total of 503 TB patients and 494 healthy controls were enrolled in this case-control study. Mass spectrometry (MS) technology was applied to genotype rs2297136 and rs4143815 of PD-L1 genes. The associations between SNPs and TB were assessed using unconditional logistic regression analysis. Marginal structural linear odds models were used to estimate the gene-environment interactions. Compared with genotype CC, genotypes GG and CG + GG at rs4143815 locus were significantly associated with susceptibility to TB (OR: 3.074 and 1.506, respectively, P < 0.05). However, no statistical association was found between rs2297136 SNP and TB risk. Moreover, the relative excess risk of interaction (RERI) between rs4143815 of the PD-L1 gene and cooking with solid fuel was 2.365 (95% CI 1.922,2.809), suggesting positive interactions with TB susceptibility. In conclusion, the rs4143815 polymorphism of the PD-L1 gene was associated with susceptibility to TB in Chinese Han populations. There were significantly positive interactions between rs4143815 and cooking with solid fuel.
Acneiform eruptions with combination targeted cancer therapy in colorectal cancer patients
Authors: Ibraheim Marina K , Lo Jonathan , Gupta Rohit , Parseghian Christine , Patel Anisha B 
Published: May 2022

Abstract
Purpose: Epidermal growth factor receptor inhibitors (EGFRI) can be used with pathway inhibitors, including mitogen-activated protein kinase kinase inhibitors (MEKIs), BRAF inhibitors (BRAFIs), and checkpoint inhibitors such as programmed death-ligand 1 (PD-L1) and programmed cell death protein 1 (PD-1) to treat colorectal cancer. These can precipitate treatment-resistant acneiform eruptions, prompting dose modification or discontinuation. Predicting the likelihood of severe rash development and crafting effective treatments may promote adherence to life-saving chemotherapy. Methods: An Institutional Review Board approved retrospective chart review of patients with colorectal cancer treated with EGFRI or MEKI in combination with HER2, BRAF, PI3K, or checkpoint inhibitors between January 1, 2016 and January 1, 2020 was performed. Surrogates for rash severity were investigated, including lower extremity involvement, utilization of oral steroids or retinoids, dose modification, and incidence of superinfection. Results: Of 122 patients treated with combination therapy, 105 developed a rash, and 87 developed an acneiform eruption. Common combinations included MEKI/PD-LI, EGFRI/MEKI, and MEKI/PD-1I. Patients treated with EGFRI/MEKI developed the most severe rashes (p=0.02). Lower extremity involvement was more frequent with EGFRI/MEKI compared to alternative combinations (p=0.05). Drug holiday correlated with all rash severity surrogates, including rash grade, lower extremity involvement, oral steroid or retinoid use, and incidence of superinfection. Use of oral steroids or retinoids was associated with development of superinfection (p=0.002). Prophylactic tetracycline use did not impact rash severity or rash incidence. Conclusion: This is the first descriptive analysis to characterize acneiform eruptions for patients with colorectal cancer on combination cancer therapy. Approximately 85% of patients developed a cutaneous toxicity with, what appears, to be synergistic effects of EGFRI and MEKI combination therapy causing the most severe eruptions. Superinfection rate correlated to systemic therapy use beyond oral tetracyclines. Further investigation into the utility of prophylactic oral tetracyclines in this population is needed.
Preparation of Environmentally Friendly Method of zinc oxide (ZnO) nanoparticles from osmium gratissimum (Damaakasee) plant leaf extracts and its antibacterial Activities
Authors: Saka Abel , Jule Leta Tesfaye , Gudata Lamessa , Seeivasan Venkatesh , N Nagaprasad , Ramaswamy Krishnaraj 
Published: May 2022

Abstract
Biological approach of Zinc oxide (ZnO) Nano particles by using Ocimum gratissimum (Damaakasee) leaf extracts were deposited and galvanized at three different heats. Zinc-nitrate hexa-hydrate (ZnNO3.6H2O) is used as metallic precursor source. The UV-Vis absorbance crests at 3oo nm specify a blue alteration in exciton preoccupation. Scanning electron microscope (SEM) micrographs demonstrates the Nano particles to be poly disseminated and a widespread assortment of magnitudes with slighter Nano particles grouping round greater micro-meter sized ones with shrill ends. A galvanizing hotness of 200 ℃ occasioned in the sintering of Nano particles nonetheless Nano particles galvanizing at 150 ℃ had leaky shells signifying a conceivable in radar tenders. An x-ray diffraction (XRD) array reveals that the Zinc Oxide Nano particles have the hexagonal wurzite configuration and of worthy crystal shape. Attained crystals sizes greater than before with annealing temperature and were in the range 18.66 nm to 27. 26 nm and average size 23.4 nm. The photo-luminescence (PL) continuums indicates (2) emanation crests. The photo-luminescence (PL) crowning strengths were reliant on the galvanizing temperatures with the UV- radiation crests for greater galvanizing temperatures demonstrating a phantom shift near lower wave lengths. The anti-bacterial activity of Zinc Oxide Nano particles gained from Ocimum gratissimum leafs against Escherichia coli and staphylococcus aureus were considered. The anti-bacterial bustle also displays virtuous zone of reserve. Green approach method encouraging for Synthesization of ZnO Nano particles in anti-bacterial bustle and illustrate the same result. This consequence validates that the biological prepared Nanoparticles initiate to have therapeutic applications and is biological degradable in microbial.
Search and Processing of Holliday Junctions within Long DNA by Junction-Resolving Enzymes
Authors: Kaczmarczyk Artur , Déclais Anne-Cécile , Newton Matthew , Boulton Simon , Lilley David , Rueda David 
Published: May 2022

Abstract
Resolution of four-way Holliday junctions is a critical intermediate step of homologous recombination. DNA junctions must be processed by abundant junction-resolving endonucleases to cleave the covalent link between two chromosomes. Although the catalytic activity and interaction mode of endonucleases with Holliday junction have been characterized in great detail by biochemical and structural studies, it remains unclear how the enzymes find their substrate located within kilobase pairs of duplex DNA. Here, we present a complete single-molecule reaction trajectory for a junction-resolving enzyme finding and cleaving a Holliday junction. We employed correlative optical tweezers with confocal fluorescence microscopy to track a single dimer of endonuclease I on the DNA template in real-time. We observed that the enzyme binds remotely to the dsDNA and then undergoes 1D diffusion. Upon encountering the four-way junction, a catalytically impaired endonuclease I mutant remains bound at that point for long periods. An active enzyme, however, cleaves the junction after a few seconds. When an N-terminal truncated endonuclease mutant is used, the enzyme fails to diffuse along dsDNA and dissociates after short periods of time, indicating that DNA encirclement by the disordered N-terminus is a key requirement for 1D diffusion and efficient target localisation. Quantitative analysis of each of these stages revealed a comprehensive description of the facilitated diffusion mechanism. We show that the eukaryotic junction-resolving enzyme GEN1 also undergoes facilitated diffusion on dsDNA until it becomes located at a junction, so that the general resolution trajectory is likely to be applicable to most junction resolving enzymes.
Luteolin-7-O-glucoside Corrects the Disorder of Glucose Metabolism in Treating Respiratory syncytial virus Pneumonia
Authors: Li Wei-Feng , Chen Yan-Zhen , Ling Xiao-Yin , Sun Ya-Lei , Yuan Bing , Tao Jia-Lei 
Published: May 2022

Abstract
Background
Respiratory syncytial virus (RSV) pneumonia is the main causes of acute bronchiolitis in infants. Luteolin-7-O-glucoside (LUT-7G) is a natural flavonoid, which exists in a variety of plants and has a variety of biological activities. The purpose of this study was to reveal the therapeutic effect of LUT-7G on RSV pneumonia from the perspective of glucose metabolism.
Methods
Mice model of RSV pneumonia were constructed and administered LUT-7G intervention (60mg/kg·d− 1). The pathological injury of lung tissue was observed by HE staining. Real-time qPCR was used to detect inflammatory factors and RSV surface protein. Then the metabolites of glycolysis and tricarboxylic acid cycle (TCA) in lung tissue and serum of mice were detected by gas chromatography-mass spectrometry (GC-MS) to evaluate the regulatory effect of LUT-7G on glucose metabolism under virus infection.
Results
Pharmacodynamic studies show that LUT-7G could reduce the inflammatory injury of lung tissue by up-regulating IL-10 mRNA, reducing RSV G, F protein and pro-inflammatory factor TNF-α and TGF-β mRNA. Target metabonomics showed that RSV infection could up-regulate the levels of glycolysis and TCA metabolites in lung tissue and serum, while LUT-7G could improve the disorder of glucose metabolism and partially reverse the upregulation of glucose, lactate and malate in lung tissue.
Conclusion
LUT-7G can improve the pathological injury of lung tissue induced by RSV by regulating glucose metabolism and inhibiting inflammation.
A coumarin-azo derived colorimetric chemosensor for Hg 2+ detection in organic and aqueous media and its extended real-world applications
Authors: Battison Aidan , Schoeman Stiaan , Mama Neliswa 
Published: May 2022

Abstract
Pollution caused by the release of toxic heavy metals into the environment by industrial and farming processes has been regarded as a major problem worldwide. This has attracted a great deal of attention into restoration and remediation. Mercury is classified as a toxic heavy metal which has posed significant challenges to public and environmental health. To date, conventional methods for mercury detection rely on expensive, destructive, complex, and highly specialized methods. Evidently, there is a need to develop systems capable of easily identifying and quantifying mercury within the environment. In this way, organic-based colorimetric chemosensors are gaining increasing popularity due to their high sensitivity, selectivity, cost-effectiveness, ease of design, naked-eye, and on-site detection ability. The formation of coumarin-azo derivative AD1 was carried out by a conventional diazotization reaction with coumarin-amine 1c and N,N-dimethylaniline. Sensor AD1 displayed remarkable visual colour change upon mercury addition with appreciable selectivity and sensitivity. The detection limit was calculated as 0.24 µM. Additionally, the reversible nature of AD1 allowed for the construction of an IMPLICATION type logic gate and Molecular Keypad Lock. Chemosensor AD1 displayed further sensing applications in real-world water samples and towards on-site assay methods. Herein, we describe a coumarin-derived chemosensor bearing an azo (N = N) functionality for the colorimetric and quantitative determination of Hg2+ in organic and aqueous media.
Phytochemicals Quantification, TLC and Antimicrobial Assessment of the Leaves and Fruit Extracts of Lasimorpha Senegalensis (Schott) Araceae
Authors: BUNU SAMUEL JACOB , Miediegha Oyeintonbara , Nwankwo Ogechukwu L. , Ozor Martins O. 
Published: May 2022

Abstract
Background: Lasimorpha senegalensis is a perennial herbaceous plant that grows in marshy places and produces a clump of leaves from a short, thick, strongly stoloniferous rhizome. It's been used to treat gonorrhea and dysentery, among other ailments. The leaves can also be used to treat coughs and uneasiness and agitation in bigger dosages. It's also given to pregnant women to help them deliver more quickly. The study aimed to conduct phytochemical screening and evaluate the antimicrobial properties of Lasimorpha senegalensis. The leaves and fruit parts Lasimorpha senegalensis (Schott) Araceae were collected at Korokorosei community in Bayelsa State, air-dried, pulverized and extracted using dichloromethane, ethanol, methanol and water. Flavonoids, saponins, tannins, ketones, and cardiac glycosides were all found to be present. Preliminary thin layer chromatographic screening of the extracts was also done, as well as the antibacterial evaluation of the different fractions using the agar diffusion technique.Results: The phytochemical screening for tannins, cardiac glycoside, ketones, and flavonoids were positive. Inhibition zones for the aqueous fraction at 62.5, 125, 250 and 500 mg/ml for E. coli was 32, 22, 18 and 11 mm respectively, while 8, 9, 10 and 14 mm for Pseudomnas aeruginosa. For the methanolic fraction, at the above concentrations, 10, 11, 14 and 14 nm was observed as zones of inhibition on Escherichia coli, 14, 19, 15, and 20 nm for Pseudomnas aeruginosa, and 19, 20, 20, and 20 nm for Staphylococcus aureus, respectively. Furthermore, in the dichloromethane fraction, 13, 20, 19, and 15 nm were observed as zones of inhibition on Escherichia coli, 8, 10, 15, and 15 nm for Pseudomnas aeruginosa, and 20, 15, 20, and 18 nm for Staphylococcus aureus, respectively.Conclusion: It is evident, from the results obtained that the leaf portion of the plants can be used in the treatment of infections caused by E. col, Pseudomonas aeruginosa, and Staphylococcus aureus. With the high amounts of flavonoids observed, it can possibly be employed as an antioxidant and a cardioprotective.
Developing and validating an assessment tool on Knowledge, Attitude, and Practice of Central Line- Associated Bloodstream Infection of ICU Nurses
Authors: Dang Fangping , Gao Jing , Ding Nannan , Zhai Tiantian , Li Huiju , Ran Xingwu 
Published: May 2022

Abstract
Background
Because limited assessment tool is available regarding knowledge, attitude, and practice (KAP) of Central-Line Associated Bloodstream Infection (CLABSI) of intensive care unit (ICU) nurses, we developed and validated an assessment tool on KAP of CLABSI of ICU Nurses.
Methods
Based on an extensive literature search, we initially developed this questionnaire. Pilot study were performed with the initial questionnaire. Then we evaluated the reliability and validity of the new questionnaire. We performed confirmatory factor analysis and exploratory factor analysis. The correlation coefficients were calculated. A test-retest was done to establish the reliability and to refine the items further.
Results
Initially, 54 items were formed in the questionnaire. The questionnaire was sent out twice and statistical analyses were made, reducing the number of items. We performed the pre-investigation and 255 participants responded to the questionnaire items. The overall content validity of the questionnaire was 0.95. The KMO value was 0.855, and the chi-square value of Bartlett's sphere test was 1479.609 (P˂0.05). After the principal component analysis extraction method and the maximum variance orthogonal rotation, four common factors were extracted, and the cumulative variance contribution rate was 60.052%. The knowledge, attitude, and practice dimensions and the overall Cronbach's coefficient of the questionnaire were 0.927, 0.778, 0.923, and 0.946, respectively. Two weeks later, the test-retest reliability was 0.931, 0.825, 0.934, and 0.945, respectively.
Conclusions
A assessment tool for ICU nurses about KAP of CLABSI was developed, which included three domains covering knowledge, attitude, and practice. The questionnaire’s validity, and reliability have been verified and can be used in clinical practice. The establishment of the tool will facilitate accurate monitoring for nursing managers to grasp the status quo about the KAP of CLABSI among ICU nurses so that nurses can be trained in a targeted manner.
Foveal morphology of retinal layers in 4 to 6-year-old children with history of retinopathy of prematurity: J-CREST study
Authors: Nishi Tomo , Ueda Tetsuo , Mizusawa Yuutaro , Shinomiya Kayo , Mitamura Yoshinori , Kimura Naoki , Gomi Fumi , Miki Akiko , Nakamura Makoto , Kinoshita Takamasa , Obata Shumpei , Ohji Masahito , Tsuji Takuya , Yoshida Shigeo , Ogata Nahoko 
Published: May 2022

Abstract
The most effective treatment for retinopathy of prematurity (ROP) is considered as the laser therapy. We compared the foveal morphology of the retina in eyes with a history of ROP with that of full-term children. This was a cross-sectional comparative study. Seventy-eight patients with a history of ROP, aged 4–6 years, were included. Among them, 45 underwent laser treatment for ROP. The clinical findings and retinal morphology in these patients were compared with that of 33 patients who had spontaneous regression of ROP and 30 age-matched full-term controls. All patients with ROP had 20/40 or better best-corrected visual acuity (BCVA). The foveal thickness was significantly thicker and foveal depression was significantly shallower in laser-treated ROP eyes than in regressed ROP eyes and controls. The outer nuclear layer was significantly thicker, and the inner segment (IS) of the photoreceptors and the inner retinal layer (IRL) were significantly longer in the laser-treated ROP eyes than that in the control eyes. In ROP patients and controls, BCVA was associated with the foveal depression, foveal depression was associated with the gestational age and IS thickness was associated with ROP stage. Our results suggest that prematurity and laser treatment affect the foveal morphology and BCVA.