bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complications of One-step Button Percutaneous Endoscopic Gastrostomy in Children
Authors: Jean-Bart Charlotte , Aumar Madeleine , Ley Delphine , Antoine Matthieu , Cailliau Emeline , Coopman Stéphanie , Guimber Dominique , Ganga Serge , Turck Dominique , Gottrand Frédéric 
Published: November 2022

Abstract
Purpose
To assess the complications of one-step button percutaneous endoscopic gastrostomy (B-PEG) and determine risk factors for developing stomal infections or gastropexy complications.
Methods
A retrospective study of 679 children who underwent a B-PEG procedure in a single tertiary care center over a 10-year period to December 2020 was conducted. Patient characteristics, early complications (occurring ≤7 days after the procedure), late complications (> 7 days after the procedure), and outcomes were collected from medical records. A list of potential risk factors, including age at procedure, prematurity, underlying neurological disease, and undernutrition, was determined a priori.
Results
At least 1 year of follow-up was available for 513 patients. Median follow-up duration was 2.8 years (interquartile range 1.0–4.9 years). Major complications were rare (< 2%), and no death was related to B-PEG. Early complications affected 15.9% of the study population, and 78.0% of children presented late complications. Development of granulation tissue was the most common complication followed in frequency by tube dislodgment and T-fastener complications. Only 24 patients (3.5%) presented stomal infections. Young age at the time of PEG placement (odds ratio (OR) 2.34 [1.03–5.30], p = .042) was a risk factor for developing peristomal infection. T-fastener migration occurred in 17.3% of children, and we found underlying neurological disease was a protective factor (OR 0.59 [0.37–0.92], p = .019).
Conclusion
B-PEG is a safe method and associated with a low rate of local infection. However, T-fasteners are associated with significant morbidity and require particular attention in young and premature infants.
Elevated levels of MMP12 sourced from macrophages are associated with poor prognosis in urothelial bladder cancer
Authors: Lord Martin , Kerzeli Iliana K , Türker Polat , Kostakis Alexandros , Malmström Per-Uno , Hemdan Tammer , Mezheyeuski Artur , Ward Douglas , Bryan Richard T , Segersten Ulrika , Mangsbo Sara M 
Published: November 2022

Abstract
Background
Urothelial bladder cancer is most frequently diagnosed at the non-muscle-invasive stage (NMIBC). However, recurrences and interventions for intermediate and high-risk NMIBC patients impact quality of life. Biomarkers for patient stratification could help to avoid unnecessary interventions whilst indicating aggressive measures when required.
Methods
In this study, immuno-oncology focused, multiplexed proximity extension assays were utilised to analyse plasma (n=90) and urine (n=40) samples from 90 newly-diagnosed and treatment-naïve bladder cancer patients. Public single-cell and bulk RNA-sequencing data from patient tumour tissues and murine OH-BBN-induced urothelial carcinomas were also explored.
Results
Plasma from muscle-invasive, urothelial bladder cancer patients displayed higher levels of MMP7 (p=0.028) and CCL23 (p=0.03) compared to NMIBC patients, whereas urine displayed higher levels of CD27 (p=0.044) and CD40 (p=0.04) in the NMIBC group by two-sided Wilcoxon rank-sum tests. Random forest survival and multivariable regression analyses identified increased MMP12 plasma levels as an independent marker (p<0.001) associated with shorter overall survival (HR=1.8, p<0.001, 95% CI:1.3-2.5); this finding was validated in an independent patient cohort. Single-cell transcriptomics analyses indicated tumour-infiltrating macrophages as a putative source of MMP12.
Conclusions
The measurable levels of tumour-localised, immune-cell-derived MMP12 in blood suggest MMP12 as an important, tumour-microenvironment-related biomarker that could complement histopathology-based risk stratification and represent a pharmacological target in urothelial bladder cancer.
Light intensity-dependent changes in the photochemistry of photosystem II, cell constituents, and lipid accumulation in the Wild-type (WT) and DCMU-tolerant mutant of green alga Scenedesmus vacuolatus
Authors: Gupta Neha , Yadav Nisha , Singh D.P , Pathak Vinayak. Vandan , Ahmad Shamshad 
Published: November 2022

Abstract
Microalgae utilize light energy for photosynthesis; however, variations in light intensities can have antagonistic and synergistic effects on its growth and cell constituents. In the present experimental study, photodynamic effects of light intensity on the growth, cell constituents, photochemistry of PS II, and lipid accumulation were studied in wild-type (WT) and DCMU-tolerant mutant strains of S. vacuolatus. Microalgal algal cells (WT and DCMU-tolerant mutant) were exposed to light intensities ranging from 10 to 100 µmol m− 2 s− 1 and cultured in a BG-11 nutrient growth medium. Overall results showed higher light intensity tolerance in the mutant strain (60 µmol m− 2 s− 1) than the WT (40 µmol m− 2 s− 1). The photosynthetic parameters derived from chlorophyll fluorescence induction kinetics (OJIP), non-photochemical quenching (NPQ), and Light curve (rETR) revealed better photosynthetic performance by the mutant strain than the WT under the high light stress. Thus, it was inferred that, unlike the WT, a better photosynthetic efficiency coupled with an improved photo-protection mechanism in the mutant strain at higher light intensities might be contributing to an enhanced level of cell constituent and lipid accumulation in the mutant strain.
Voxel-based Dosimetry Predicting Treatment Response and Related Toxicity in HCC Patients Treated with Resin-based Y90 Radioembolization: A prospective, single-arm study
Authors: Kokabi Nima , Arndt-Webster Linzi , Chen Bernard , Brandon David , Sethi Ila , Davarpanahfakhr Amir , Galt James , Elsayed Mohammad , Bercu Zachary , Cristescu Mircea , Kappadath S Cheenu , Schuster David M 
Published: November 2022

Abstract
Background:
There is an increasing body of evidence indicating Y90 dose thresholds for tumor response and treatment related toxicity. These thresholds are poorly studied in resin Y90 particularly in hepatocellular carcinoma (HCC).
Purpose:
To evaluate the efficacy of prospective voxel-based dosimetry for predicting treatment response and adverse events (AE’s) in patients with HCC undergoing resin-based Y90 radioembolization.
Materials and Methods:
This correlative study was based on a prospective single-arm clinical trial (NCT04172714) which evaluated the efficacy of low/scout (15 mCi) activity of resin-based Y90 for treatment planning. Partition model was used with the goal of tumor dose (TD) > 200 Gy and non-tumoral liver dose (NTLD) < 70 Gy for non-segmental therapies. Single compartment dose of 200 Gy was used for segmentectomies. Prescribed Y90 activity minus scout activity was administered for therapeutic Y90 followed by Y90-PET/CT. Sureplan® (MIM Software, Cleveland, OH) was used for dosimetry analysis. Treatment response was evaluated at 3 and 6 months. Receiver operating characteristic curve determined TD response threshold for objective (OR) and complete response (CR) as well as non-tumor liver dose (NTLD) threshold that predicted AE’s.
Results:
N = 30 patients were treated with 33 tumors (19 segmental and 14 non-segmental). One patient died before the first imaging and clinical follow-up was excluded from this analysis. Overall, 26(81%) of tumors had OR and 23(72%) had CR. Mean TD of 253 Gy predicted OR with 92% sensitivity and 83% specificity (area under the curve (AUC = 0.929, p < 0.001). Mean TD of 337 Gy predicted CR with 83% sensitivity and 89% specificity (AUC = 0.845, p < 0.001). Mean NTLD of 81Gy and 87 Gy predicted grade 3 AE’s with 100% sensitivity and 100% specificity in the non-segmental cohort at 3- and 6-month post Y90, respectively.
Conclusion:
In patients with HCC undergoing resin-based Y90, there are dose response and dose toxicity thresholds directly affecting outcomes.
Seasonal Monitoring of Microbial Activity Using Conventional Approaches in a Full-Scale Urban Biological Wastewater Treatment Plant
Authors: Demirbilek Deniz , İpek Ubeyde , Yetiş Ülkü 
Published: November 2022

Abstract
Activated sludge processes contains various groups of microorganisms with different metabolic properties, which are responsible for contaminants removal. Therefore, it is important to elucidate the general structure and functional properties of biomass in activated sludge processes. For this purpose, a full-scale domestic biological wastewater treatment plant in Tunceli (Turkey), Tunceli WWTP, was monitored to observe seasonal variations in process performance and biomass properties for a year. It was observed that nitrifying bacteria developed abundantly in the rainy and cool spring season as they were suppressed in summer because their large losses took placed due to an environment containing high alkalinity values. In September, aerobic heterotrophic, nitrify, denitrify, and anaerobic activities increased. It can be said that mature biomass contained young and active levels in an environment in which the sludge volume index (SVI) value increased to 196 mL/g. As a result of the improvement in the structural and functional properties of biomass, the nitrogen removal efficiency reached to 99%. Throughout whole study, the structural improvement observed in biomass reflected in its removal activity. The amount of biomass and removal activity decreased with the abundance of organic matter in the influent at the period in which biomass was closer to being categorized in the aged sludge class. The results showed that as the lowest mixed liquid volatile suspended solids (MLVSS) value of the year was 400 mg/L in November, MLVSS value reached the highest amount (1,400 mg/L) in December which aerobic heterotrophic activity accelerated with a decrease in organic matter level.
Posteroanterior scout view of pediatric patients using computed tomography: Validation using pediatric anthropomorphic phantoms
Authors: Arao Keiko , Masuda Takanori , Funama Yoshinori , Nakaura Takeshi , Sato Tomoyasu , Masuda Shouko , Oku Takayuki , Gotanda Rumi , Imaizumi Hiromasa , Arao Shinichi , Ono Atsushi , Hiratsuka Junichi , Awai Kazuo 
Published: November 2022

Abstract
Background: To compare the effect of obtaining the anteroposterior scout view (APSV) with that of posteroanterior scout view (PASV) on mammary gland dose in pediatric patients.
Methods: We used a pediatric anthropomorphic phantom with a 64 detector-row computed tomography (CT) scanner using the APSV or PASV protocol of the chest. A real-time skin dosimeter was placed on the scanner gantry and pasted onto the phantom surfaces of the right and left mammary glands. We compared the real-time skin dosimeter values in the right and left mammary glands between the APSV and PASV protocols at different tube voltages.
Results: Compared with APSV, it is possible to reduce the exposure dose by approximately 80% using PASV at different tube voltages (p < 0.05).
Conclusion: Obtaining the PASV was helpful in reducing the mammary gland dose by approximately 80% in pediatric patients than that of APSV without deteriorating the image quality of the main scan.
Exploration of KIR Genes and Hematological-related Diseases in Chinese Han Population: a Multi-center Retrospective Analysis
Authors: Li Ye-Mo , Li Yu-Xia , Hu Xiao-Zhuang , Li Dai-Yang , An Lin , Yuan Zhi-Yang , Liu Zhong-Liang , Du Ke-Ming , Zheng Zhong-Zheng 
Published: November 2022

Abstract
The function of natural killer (NK) cells has previously been implicated in hematopoietic-related diseases. Killer immunoglobulin-like receptors (KIR) play an important role in NK cells after hematopoietic stem cell transplantation. To explore the immunogenetic predisposition of hematopoiesis-related diseases, herein, a multi-center retrospective study in China was conducted, analyzing and comparing 2519 patients with hematopathy (mainly, acute lymphoblastic leukemia, acute myeloid leukemia, aplastic anemia, and myelodysplastic syndrome) to 18108 individuals without known pathology. Genotyping was performed by polymerase chain reaction with specific sequence primers (PCR-SSP). As a result, we discovered four genes including KIR2DL5 (OR: 0.74, 95% CI: 0.59–0.93; Pc = 0.0405), 2DS1 (OR: 0.74, 95% CI: 0.59–0.93; Pc = 0.0405), 2DS3 (OR: 0.58, 95% CI: 0.41–0.81; Pc = 0.0180), and 3DS1 (OR: 0.74, 95% CI: 0.58–0.94; Pc = 0.0405) to be protective factors that significantly reduce the risk of aplastic anemia. Our findings offer new approaches to immunotherapy for hematological-related diseases. As these therapies mature, they are promising to be used alone or in combination with current treatments to help to make hematopathy a manageable disease.
Biomarkers of GPC3, DCP and VEGF in Hepatocellular Carcinoma: Early Diagnostic and Prognostic Significance in the Chinese Population
Authors: Zhu Yangbo , Qin Jiayi , Ye Ziqi , Zhou Lin , Ling Qi 
Published: November 2022

Abstract
Background: Glypican-3 (GPC3), des-γ-carboxyprothrombin (DCP) and vascular endothelial growth factor (VEGF) are potential biomarkers for the early diagnosis and prognosis of hepatocellular carcinoma (HCC), especially in AFP negative patients. However, their sensitivity and accuracy as biomarkers are still needed to be compared and verified by large clinical samples in Chinese population.
Methods: ELISA was used to evaluate the GPC3, DCP and VEGF in the serum of 136 HCC patients, 47 liver cirrhosis patients and 46 healthy controls. Meanwhile, the risk factors and mortality for HCC recurrence were also investigated to obtain some prognostic variables according to the histopathological evaluation and follow-up data.
Results: The GPC3 and VEGF serum levels were significantly sensitive and specific compared with those of AFP and DCP for the diagnosis of HCC based on cut-off values. Compared with VEGF, the maximum area under the curve (AUC) of serum GPC3 level indicated most accurately in the diagnosis of HCC. In addition, GPC3 level was increased in 50.6% (39 / 77) of HCC patients with negative serum AFP. The risk factors for recurrence were high serum levels of tumor biomarkers, including DCP (>350 mAu/ml) and VEGF (>112 pg/ml) which were significantly higher in patients with microvascular invasion (MVI) than in those without MVI (P<0.01). Moreover, the expression of VEGF in various types of tumor invasion was also significantly different (P < 0.05, type 3 vs. type 1).
Conclusions: Serum GPC3 and VEGF levels have the potential to be effective biomarkers for the early diagnosis of HCC, especially for these with normal serum AFP. Serum DCP and VEGF levels would be non-invasive prognostic biomarker substitute for tumor invasiveness and MVI, which were tightly correlated with postoperative recurrence of HCC.
Evolution of surface-borne vibrational communication in fungus-growing ant societies
Authors: Murakami Takahiro , Sakamoto Hironori , Higashi Seigo 
Published: November 2022

Abstract
In ants, chemical communication, through pheromones for example, was thought to be the main communication tool in ant societies; however, a major question is whether chemical substances alone can control highly differentiated social behaviours. In this study, we focused on the surface-borne vibrational signals of fungus-growing ants, key organisms in the Neotropical ecosystem, and conducted a detailed analysis of the signals. Our results indicate that in fungus-growing ants, vibrational communication is a much more frequent and critical factor than previously believed. The frequency of vibrational signal recordings from the ants were found to be significantly correlated with social structure complexity. Structural analysis indicated that both the area and number of slits in the stridulatory organs were significantly correlated with body size. The ability of leaf-cutting ants to maintain fungus gardens was significantly lower in the vibration-inhibited group than in the pheromone secretion-inhibited group. These results suggest that ants that have become more communicative may induce altruistic behaviours and create more complex societies. The findings of this study may be applicable not only to social evolution studies but also for effectively controlling ant behaviours.
Similarity Matching of Medical Question Based on Siamese Network
Authors: Li Qing , He Song 
Published: November 2022

Abstract
Background
With the rapid development of the medical industry and the gradual increase in people's awareness of their health, the use of the Internet for medical question and answer, to obtain more accurate medical answers. It is necessary to first calculate the similarity of the questions asked by users, which further matches professional medical answers.
Method
This paper focuses on building a bidirectional gated recurrent unit(BiGRU) deep learning model based on Siamese network for medical interrogative similarity matching, using Word2Vec word embedding tool for word vector processing of ethnic-medical corpus, and introducing an attention mechanism and convolutional neural network. Bidirectional gated recurrent unit extracts contextual semantic information and long-distance dependency features of interrogative sentences; introducing an attention mechanism can give higher weight to keywords in interrogative sentences and further improve the recognition of similar words in interrogative sentences; Convolutional neural network takes into account the local information of interrogative sentences and can capture local position invariance, allowing feature extraction for words of different granularity through convolutional operations; By comparing the Euclidean distance, cosine distance and Manhattan distance to calculate the spatial distance of medical interrogatives, the Manhattan distance produced the best similarity result.
Result
Based on the ethnic medical question dataset constructed in this paper, the accuracy and F1-score reached 97.24% and 97.98%, which is a significant improvement compared to several other models.
Conclusion
By comparing with other models, the model proposed in this paper has better performance and achieve accurate matching of similar semantic question data of ethnic medicine.
Autosomal recessive bestrophinopathy combined with neurofibromatosis type 1 in a patient
Authors: Zhao Bo , Chen Lian , Zhang Peng , He Ke , Lei Min , Zhang Juan 
Published: November 2022

Abstract
Background

Neurofibromatosis type 1 (NF1) is a multisystem genetic disorder that may affect multiple systems of the body. Autosomal recessive bestrophinopathy (ARB) is a rare retinal dystrophy caused by autosomal recessively mutations in bestrophin 1 (BEST1) gene. So far, we have not retrieved any case report of the same patient with both NF1 and BEST1 gene mutations.

Case presentation

An 8-year-old female patient with café-au-lait spots, freckling on skin presented to our ophthalmology clinic for routine ophthalmological examination. Her best corrected visual acuity (BCVA) was 20/20 in both eyes. Slit-lamp examination of both eyes revealed few yellowish-brown dome-shaped Lisch nodules over the iris surface. Fundus examination was notable for bilateral confluent yellowish subretinal deposits at macula, few yellow flecks at temporal retina, and cup-to-disc ratio of 0.2. Optical coherence tomography (OCT) revealed subretinal fluid (SRF) involving the fovea, elongated photoreceptor outer segments and mild intraretinal fluid (IRF) at bilateral macula. Fundus autofluorescence demonstrated hyperfluorescence in the area corresponding to the subretinal deposits. Whole-exome sequencing and Sanger sequencing were used to investigate genetic mutation in the patient and her parents. A BEST1 gene heterozygous missense c.604C > T (p.Arg202Trp) was identified in the patient and her mother. Also, the patient carries an NF1 nonsense mutation c.6637C > T (p.Gln2213*) with the mosaic generalized phenotype. There were no visual impairments or obvious neurological, musculoskeletal, behavioral or other symptoms in this patient, so she was managed conservatively and advised to follow up regularly for a long time.

Conclusions

ARB and NF1, which are caused by two different pathogenic gene mutations, have rarely coexisted in the same patient. The discovery of pathogenic gene mutations may play a crucial role in more accurate diagnostics and genetic consultations for individuals and their families.
Calcium ions improve organic carbon stability in bauxite residue through alkaline minerals
Authors: Xue Shengguo , Zhang Yufei , Jiang Jun , Li Feng , Guo Xuyao , Zhu Feng , Chen Hualin , Wu Yujun 
Published: November 2022

Abstract
Purpose
The interaction of minerals and organic matter significantly affects the stability of organic carbon, which is key to the rehabilitation of bauxite residue. However, due to the microscopic structure of minerals and the chemical composition of dissolved organic matter (DOM), the interaction mechanism between alkaline minerals and DOM in the presence of calcium ions that are widely used as amendments is still poorly understood.
Methods
In this work, the adsorption mechanism of organic matter by sodalite and cancrinite in the presence of calcium ions was investigated by fourier transform infrared spectroscopy (FTIR), excitation-emission matrix fluorescence (EEMs), thermogravimetric analysis (TG-DSC) and atomic force microscope (AFM).
Results
The calcium ions can act as bridges to connect the negative sites on the mineral surface with the polysaccharide functional groups of organic matter, thus allowing the DOM to enter the pore channels of mineral structure to sequester organic carbon via ligand-exchange and/or electrostatic interactions. Furthermore, DOM located in the pore structure within cancrinite may be better protected due to physical isolation. The 3D view of the interacted minerals-organic matter assemblages provided visual evidence that the adsorption sites distributed on the surface of cancrinite increased by the action of calcium ions, with different adsorption capacity of individual sites for DOM.
Conclusions
The calcium ions enhance the ability of alkaline minerals to trap dissolved organic carbon, and the work highlighted the significance of organo-mineral associations in the microscopic mineral structure for the organic carbon sequestration in the revegetation process of bauxite residue disposal areas.