bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulatory effect of sodium butyrate on anticancer activity of abemaciclib in MDA-MB- 231 human breast cancer cells
Authors: Elnozahi Neveen A. , ELAziz Esraa A. Abd , Helmy Maged W. , Bistawroos Azza E. 
Published: May 2022

Abstract
Triple negative is the most aggressive subtype of breast cancer with its treatment limited only to chemotherapy and radiotherapy. The effect of abemaciclib and /or sodium butyrate on MDA-MB-231 triple negative breast cancer cells was investigated. Using MTT assay, IC50 for the growth inhibitory effect of abemaciclib, sodium butyrate, and their combination were 14.55 µM, 7.08 mM, 3.743 mM, respectively. The combination showed a synergistic interaction with a decrease in IC50 to 2.55 µM of abemaciclib and 3.74 mM of butyrate. Three replica of four groups of cancer cells were treated for 48hr with IC50 of either abemaciclib or butyrate or their high or low doses combinations. A fifth group was treated with complete medium and served as control. Cell migration, protein expression level of cyclinD1, E2F2 transcription factor, phosphorylated AKT, nuclear factor kabba B (NF-κB), cyclin dependent kinase-2 (CDK2), retinoblastoma (Rb), p16INK4a, p53 and mRNA level of CDK2, p16INK4a and p53 were assessed in all treated groups. Combination treatment of abemaciclib and butyrate showed a significant attenuation of cell metastasis. The combination treatment was associated with a decrease in E2F2, CDK2, and NF-κB protein level together with an attenuation in AKT phosphorylation level. The combination also showed an elevation in Rb, p16INK4a, together with a reversal of DNA hypo-methylated state. Although abemaciclib monotherapy failed to alter CD1 or P53 levels, the combination significantly reduced CD1 level together with an increase in P53 level. In conclusion, combining butyrate with abemaciclib augmented its antiproliferative and antimetastatic effect and induced apoptotic activity.
Multiple-pinhole collimators improve intra- and between-rater agreement and the certainty of the visual interpretation in dopamine transporter SPECT
Authors: Mathies Franziska , Apostolova Ivayla , Dierck Lena , Jacobi Janin , Kuen Katja , Sauer Markus , Schenk Michael , Klutmann Susanne , Forgács Attila , Buchert Ralph 
Published: May 2022

Abstract
Background: Multiple-pinhole (MPH) collimators improve the resolution-sensitivity tradeoff compared to parallel-hole collimators. This study evaluated the impact of MPH collimators on intra- and between-rater agreement, and on the certainty of visual interpretation in dopamine transporter (DAT)-SPECT.Methods: The study included 71 patients (62.1±12.7y). Two SPECT acquisitions were performed in randomized order after a single injection of 182±9MBq 123I-FP-CIT, one with MPH and one with low-energy-high-resolution-high-sensitivity (LEHRHS) collimators. MPH projections were reconstructed with an iterative 3d Monte Carlo algorithm. LEHRHS projections were reconstructed with filtered backprojection (FBP) or with ordered-subsets-expectation-maximization and resolution recovery (OSEM). Images were visually evaluated twice by three independent raters with respect to presence/absence of Parkinson-typical reduction of striatal 123I-FP-CIT uptake using a Likert 6-score (-3=clearly normal, …, 3=clearly reduced). In case of intra-rater discrepancy, an intra-rater consensus was obtained. Intra- and between-rater agreement with respect to the Likert score (full score and dichotomized score) were characterized by Cohen’s kappa. Results: Intra-rater kappa of visual scoring of MPH/LEHRHS-OSEM/LEHRHS-FBP images was 0.84±0.12/0.73±0.06/0.73±0.08 (full score, mean of three raters) and 1.00±0.00/0.96±0.04/0.97±0.03 (dichotomized score). Between-rater kappa of visual scoring (intra-rater consensus) of MPH/LEHRHS-OSEM/LEHRHS-FBP images was 0.70±0.06/0.63±0.08/0.48±0.05 (full score, mean of three pairs of raters) and 1.00±0.00/0.92±0.04/0.90±0.06 (dichotomized score). There was a decrease of (negative) Likert scores in normal DAT-SPECT by 0.87±0.18 points from the LEHRHS-OSEM to the MPH setting. The (positive) Likert scores of reduced DAT-SPECT did not change on average.Conclusions: MPH collimators improve intra- and between-rater agreement as well as the certainty of the visual interpretation of DAT SPECT.
Statin Use and Delirium Risk: An Updated Systematic Review and Meta-Analysis
Authors: Chang Ya-Hui , Wang Jian-Ying , Peng Tzu-Rong , Lian Jia-Haur , Lee Ming-Chia , Chen Hui-Ming 
Published: May 2022

Abstract
Purpose: Findings on the association of statin use with delirium risk are inconsistent. We performed an up-to-date systematic review and meta-analysis to elucidate this association among critically ill and surgical patients.Materials and Methods: We searched PubMed, the Cochrane Library, and the EMBASE database, limiting the search to human patients and articles in English published until December 31, 2021. The odds ratio (OR) and 95% confidence interval (CI) were used to indicate the difference in the incidence of delirium between statin use and nonuse groups. A random-effects model was selected in the case of high heterogeneity of study populations. We used funnel plots, Egger’s test, Duval and Tweedie’s trim-and-fill approach, and the classic fail-safe N to assess publication bias.Findings: Of a total of 264 identified studies, 13 were selected for the qualitative review—4 RCTs and 9 observational cohort studies. Statin use was not associated with low delirium risk (pooled OR, 0·82; 95% CI, 0·64 to 1·04; p = 0·09). Substantial statistical heterogeneity was observed (I2, 90%). Visual inspection of the funnel plot of ORs from the studies revealed symmetry.Conclusions: Statin use is not associated with delirium risk. More comprehensive RCTs are required to confirm the results.
Effect of plasma welding current on heat source penetration ability of plasma-GMAW hybrid welding
Authors: Han Jiao , Han Yongquan , Sun Zhenbang , Hong Haitao 
Published: May 2022

Abstract
In the plasma-gas metal arc welding (GMAW) hybrid welding of aluminum alloy, the weld penetration under different plasma welding currents was compared. When the plasma welding current was lower than 150 A, the hybrid welding penetration was greater than the sum of the single plasma welding and the single GMAW penetration. When the plasma welding current reached 130 A, with the increase of the plasma welding current, the advantage of the weld penetration of the hybrid welding was gradually weakened compared with that of the single welding. The above change mechanism is revealed from the perspectives of heat source energy distribution and molten pool stress. The results show that when the plasma welding current is lower than 110 A, the area of the high-temperature region in the arc zone of GMAW in the hybrid welding increases compared with that in the single GMAW. The trend of arc energy transfer to the penetration direction increases under the action of arc pressure, arc shear force, electromagnetic force, and droplet impact. With the increase of plasma welding current, the high-temperature area of the GMAW arc first increases and then decreases. When the plasma current reaches 130 A, the effect of arc pressure, arc shear force, electromagnetic force, and droplet impact on increasing the penetration begins to weaken.
Characteristics of profiling the peripheral blood T‐cell receptor repertoire in patients with diffuse large B-cell lymphoma
Authors: Li Jing , Zhou Zhiyuan , Fu Xiaorui , Zhang Zhixin , Zhang Mingzhi 
Published: May 2022

Abstract
Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin’s lymphoma with strong heterogeneity and high invasiveness. The high-throughput sequencing of peripheral blood T cell receptor (TCR) can analyze the individual's immune response and directly reflect the immune status. This study aimed to detect the TCR β chain in peripheral blood of DLBCL patients and the control group, and also to analyze the characteristics of the TCR CDR3 immune repertoire of DLBCL patients before and after chemotherapy. We found that the diversity of the baseline TCR repertoire in DLBCL samples were significantly reduced than those in the control group, while there was no difference in diversity before and after chemotherapy. Patients with 53.5 years or older, stage III-IV, high IPI score and ECOG performance showed a limited TCR CDR3 repertoire. The baseline TCR diversity of patients who achieved complete remission (CR) was higher than that of the non-remission (Non-CR) group. Comparing the V gene and J gene of the CR group and the Non-CR group, it was found that the two Vβ genes have the potential to predict the therapeutic effect. In addition, standard first-line chemotherapy regimen caused changes in the frequency of T cell clones in DLBCL patients. The similarity index of CR group was lower, indicating that more changes in TCR clones occurred after chemotherapy. This study revealed the unique TCR CDR3 immune repertoire of DLBCL patients and its relationship with clinical characteristics. Dynamic monitoring of TCR diversity can more accurately predict clinical benefit.
Correlation between the outcome of vitrectomy for proliferative diabetic retinopathy and erythrocyte hematocrit level and platelet function
Authors: Sato Keiji , Kogo Jiro , Jujo Tatsuya , Sekine Reio , Uchiyama Naoto , Kakehashi Kota , Kawagoe Tatsukata , Tokuda Naoto , Kitaoka Yasushi , Takagi Hitoshi 
Published: May 2022

Abstract
We investigated whether biomarkers such as red blood cell hematocrit (Hct), platelet count (PLT), mean platelet volume (MPV), and platelet distribution width (PDW) are useful as prognostic indicators of postoperative macular edema (ME) after vitrectomy for proliferative diabetic retinopathy (PDR). Forty-eight eyes of 43 patients with PDR who underwent vitrectomy between January 2018 and May 2020 were analyzed retrospectively. We divided them into two groups according to whether treatment was required for postoperative ME and compared the relationship between Hct, PLT, MPV, and PDW and the onset of postoperative ME. The group that received postoperative treatment (group T) comprised 13 eyes of 12 patients, and the group that did not (group N) comprised 35 eyes of 32 patients. The age (years) was 53.2 ± 9.9 in group T and 60.0 ± 11.4 in group N. When the Wilcoxon rank-sum test, Pearson chi-square test, and Fisher's exact test were performed for comparison between groups, significant differences were found in Hct (p = 0.04). Multivariate logistic regression analysis was performed, and a significant difference was found in Hct (< 0.01). These results suggest that Hct might be useful as a predictor of ME after PDR surgery.
3D printed laboratory equipment to measure bulk materials in extreme conditions
Authors: Diviš Jan , Hlosta Jakub , Žurovec David , Rozbroj Jiří , Kruszelnicka Weronika , Nečas Jan , Zegzulka Jiří 
Published: May 2022

Abstract
The development of 3D printing methods and of printing materials enabled users to print 3D parts on demand, which is crucial in fast prototyping of new equipment or resolving problems with laboratory equipment both on Erath and in the universe. Due to relatively new solutions in the field of 3D printing, there are few studies on the possibility of using printed elements in measuring devices. In order to fill the gap in this area, the aim of this study was to investigate the possibility of using instruments made by material extrusion 3D printing method for measurement of selected mechanical-physical properties of bulk materials. We explore the feasibility of measuring bulk material mechanical-physical properties when there are obstacles for printing original or modified measuring instruments in common practice, such as the lower weight of the measuring instruments, the ability to pre-print a set of laboratory measuring instruments or to print the set on the spot, to replace a damaged part with a new 3D printed part on-demand, the logistical unavailability, customization of the standardized tests for better understanding of the behaviour of the particulate materials, and cheaper manufacturing cost. To achieve the goals a series of experiments such as Schulze’s ring shear tests, Freeman’s FT4 shear tests, compressibility tests, and Flow Rate and Stability tests were performed with use of original aluminium or steel made instruments and 3D printed instruments from polylactic acid and acrylic styrene acrylonitrile materials, using lunar regolith simulants LHS-1 and LMS-1 produced by CLASS Exolith Lab as a sample material. The results obtained from tests with original and printed instruments were then compared. The compared values of the effective angle of internal friction, linearized angle of internal friction, flow function, cohesion, compressibility, basic flowability energy, flow rate index, and stability index showed applicability of the 3D printed measuring instruments.
Risk factors analysis for adverse short-term prognosis in patients with contrast-induced acute kidney injury: a retrospective study
Authors: Deng Yunzhen , He Yi , Zhuang Kaiting , Wen Yanlin , Chen Junxiang 
Published: May 2022

Abstract
Aims: We aimed to evaluate risk factors influencing adverse short-term outcomes in contrast-induced acute kidney injury (CI-AKI) patients after coronary angiography (CA) or percutaneous coronary intervention (PCI). Materials and methods: We retrospectively collected 64 consecutive CI-AKI patients after CA/PCI procedure from January 2014 to November 2019. The clinical outcomes were in-hospital mortality and persistent renal dysfunction (RD). Univariable and multivariable analyses were used to identify the risk factors for in-hospital mortality and persistent RD. Results: The incidence of in-hospital mortality was 7.8% in CI-AKI patients. After adjusting potential confounders, cardiogenic shock (OR=40.5, 95% CI, 4.147–395.494, P=0.001) was the independent risk factor for in-hospital mortality. Persistent RD occurred in 35 (59%) of survival patients. After adjusting potential confounders, eGFR (OR=3.553, 95% CI, 1.497–25.416, P=0.027), duration of procedure (OR=1.037, 95% CI, 1.002–1.073, P=0.038) and contrast media category (OR=7.189, 95% CI, 1.202–42.982, P=0.031) were independent risk factors for persistent RD. Conclusion: In-hospital mortality of CI-AKI patients was associated with severe systemic hemodynamic alteration. Patients with existing renal impairment before CA/PCI were more likely to develop persistent RD, while reducing CA/PCI procedure time and use of isotonic contrast media (IOCM) might help decreasing the risk.
Bone repair of implants surfaces in rats: histomorphometric analysis, scanning electron microscopy and energy dispersion X-ray spectroscopy. A Randomized study
Authors: Francisconi Giovanna Barbosa , Cervantes Lara Cristina Cunha , Tonini Karen Rawen , Sol Izabella , Ponzoni Daniela , Junior Idelmo Rangel Garcia 
Published: May 2022

Abstract
This study aimed to characterize the newly formed bone tissue adjacent to implant surfaces histologically. The implants were previously analyzed by scanning electron microscopy and energy dispersion X-ray spectroscopy and installed in the right tibia of 75 rats divided into the: polished group, machined group and treated group. The animals were euthanized at 3, 7, 15, 21, and 40 postoperative days, and the tibias were histologically analyzed for bone/implant contact. Scanning electron microscopy of the surfaces showed topographic differences, with the smoothest and most regular surfaces in the polish group, followed by the surface of the machine group, while the treated group showed surfaces with the greatest roughness. The energy dispersion X-ray spectroscopy revealed surfaces without contamination and showed similar Titanium, Aluminum, and Vanadium contents in all groups. The implant osseointegration process showed healthy and vital bone with similar qualitative patterns in all groups. Bone cortilization occurred in contact with the implants’ titanium surfaces, even in the areas close to the tibial bone marrow. The peri-implant repair pattern was similar between all the surface types at all other timelines, with cortical bone formation in all the titanium surface of the three implant groups.
Multi-omics analyses reveal multimodal Fusobacterium DNA damage perturbations in colorectal cancer
Authors: Kataria Radhika , Quist Jelmar , Lee Sunjae , Megalios Anargyros , Sawyer Elinor , Hardiman Thomas , Liu Chang , Kelly Katherine , Portlock Theo , Alon-Maimon Tamar , Wan Jonathan , Bachrach Gilad , Shoaie Saeed , Grigoriadis Anita 
Published: May 2022

Abstract
Fusobacterium nucleatum is an opportunistic bacterial pathogen and oncogenic microbe. Using host sequencing data to systematically investigate Fusobacterium-host interactions in colorectal cancers (CRCs), we developed and orthogonally validated a multi-omics PAThogen CHaracterisation tool (PATCH) enabling gene-level annotations. In 1,020 CRC transcriptomes and 86 genomes from The Cancer Genome Atlas (TCGA), 9 Fusobacterium species were detected to gene-level resolution identifying carcinogenic virulence factors. PATCH uncovered the integration of Fusobacterium’s DNA into the host genome, specifically in non-coding regions and near Short Interspersed Elements. CRCs with integrated-Fusobacterium DNA revealed perturbations in host DNA damage response mechanisms, including deficiencies in chromosome segregation, increased microsatellite instability, elevated cytosolic DNA/RNA sensing signalling, and mutations in genes involved in oncogenic-related pathways. Fusobacterium-positive CRCs exhibited global LINE-1 hypomethylation, suggesting a potential integration path into non-coding regions. Our analytical approaches have delineated Fusobacterium’s multimodal contribution to disrupting genomic stability in CRC, which could facilitate tailored treatment options.
Advanced Finite Element Model for predicting surface integrity in high-speed turning of AA7075-T6 under dry and cryogenic conditions
Authors: Imbrogno S , Rotella Giovanna , Prete Antonio Del 
Published: May 2022

Abstract
The overall quality of a component, its mechanical performance and reliability during all its life cycle depends on several characteristics. The choice of the material plays an important role in satisfying the requirements and usually it makes the difference between failed and not failed products, however another significant impact in the material performances is characterised by the manufacturing process. Nowadays, the numerical simulation represents an important tool to quickly predict several scenario depending on process parameters changes leading to a more flexible manufacturing process and time-cost saving due to the reduced experimental tests. This work presents an innovative Finite Element Model (FEM) of high-speed turning of one of the most used aluminium alloys in aerospace field, namely the AA7075-T6. The developed simulation tool allowed to better investigate the metallurgical phenomena triggered by the vary process parameters tested and the cooling conditions used during the machining tests. A physics-based model to simulate the material behaviour has been developed and implemented via subroutine within the FEM software. The predictive capability of the model has been validated through experimental and numerical comparison of cutting forces, maximum temperature and the grain size changes.
Prognostic values and clinical relationship of AHSA1 in hepatocellular carcinoma
Authors: wang qiqi , wang changcheng 
Published: May 2022

Abstract
Objective
To investigate the expression status of the activator of Hsp90 ATPase homolog 1-like protein (AHSA1)in hepatocellular carcinoma༈HCC༉, further analyzed the relationship between AHSA1 expression level and prognosis and possible mechanism of action .
Methods
HCCDB, GEPIA and Oncomine databases were used to analyze the expression of AHSA1 mRNA in hepatocellular carcinoma and normal liver tissues. Download RNA-seq data and the clinical information of LIHC from the TCGA database, Univariate and multivariate Cox proportional hazards regression models and Kaplan–Meier plots were used to evaluate the prognostic value of AHSA1 in HCC. R software was used to construct the nomogram based on the expression level of AHSA1. The calibration curve was plotted to evaluate the consistency between the actual survival and the predicted survival. GO and KEGG gene set enrichment analysis revealed tumor-associated biological processes related to AHSA1. The TIMER2.0 and GFPIA database are used to evaluate the correlation between AHSAl and tumor immune infiltration. The correlation between AHSA1 and the expression of 8 glycolysis-related genes in HCC was studied through the GEPIA database. Screening of small molecule targeted drugs for AHSA1 through CMAP.
Results
The expression level of AHSA1 in patients with HCC was much higher than that in normal tissues༈P < 0.01). High expression of AHSA1 was associated with a worse prognosis of HCC compared low expression of AHSA1(P < 0.05). AHSA1 expression was an independent factor affecting overall survival༈HR = 1.970, p < 0.001༉. The association between AHSA1 gene expression and the risk of HCC was presented in a nomogram. The AUC of OS for 1、3 and 5 years is 0.721、0.711 and 0.725 respectively. The calibration chart shows that the predicted survival rate is in good agreement with the actual survival rate curve. GO and KEGG enrichment analysis showed that AHSAl can promote tumor progression by mediating neutrophil activation and participating in biological processes such as glycolysis and gluconeogenesis. The expression level of AHSA1 mRNA was positively associated with the degrees of immune infiltration by B cells, CD4 + T cells, regulatory T cells, macrophages, neutrophils and dendritic cells in HCC(P < 0.05). The expression of AHSA1 was significantly positively correlated with the expression of glycolysis-related genes. Etacrynic acid and Blebbistatin may be small molecule targeted drugs that can reverse the expression of AHSA1.
Conclusions
AHSA1 mRNA may be a potential oncogene in hepatocellular carcinoma, and its up-regulated expression plays an important role in the development and progression of hepatocellular carcinoma. High levels of AHSA1 mRNA expression may promote the immune infiltration of HCC and indicates the poor prognosis of HCC patients. AHSA1 might be a prognostic molecule and therapeutic target of hepatocellular carcinoma.
Heritability, productivity and economic profitability of The Biofloc Strain of Tilapia
Authors: Nugroho Estu , Kristanto Anang Hari , D Raden Roro Sri Pudji Sinarni , Gunadi Bambang , Robisalmi Adam , Pamungkas Wahyu , Setijaningsih Lies , Rifaldi Muhammad 
Published: May 2022

Abstract
The decreasing productivity and increasing environmental pressure on intensive tilapia culture in open waters, especially in floating net cages, have forced the development of a biofloc system for tilapia farming. This study was aimed to develop the superior tilapia strain suitable for the biofloc system, their productivity and economic profitability under commercial scale in the biofloc system. The selective breeding activity was carried out on 100 families reared and mated in hapa in the biofloc pond for three generations. Individual selection was conducted at the age of 6 months on the characters of standard length and body weight with the best 10% reference for formation of the next generation. After three generations, the offspring obtained from mass spawning were grown out in round-limen ponds and square-concrete ponds for productivity and profitability observations. The results showed that heritability values ​​of the biofloc tilapia strain were 0.4196 (standard length, SL) and 0.4898 (body weight, BW). The average response selection of BW and SL were 13.04% and 6.11%, respectively. Biomass gain was obtained between 19.29–28.83 kg/m3. The production multiply value was 8.07-13.59x. The average daily weight growth was between 2.8g-3.0g. The Benefit-Cost ratio valued from 1.81 to 2.71.