bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intracellular BAPTA directly inhibits PFKFB3, thereby impeding mTORC1-driven Mcl-1 translation and killing Mcl-1-addicted cancer cells
Authors: Sneyers Flore , Kerkhofs Martijn , Welkenhuyzen Kirsten , Shemy Ahmed , Voet Arnout , Eelen Guy , Dewerchin Mieke , Tait Stephen , Ghesquière Bart , Bootman Martin , Bultynck Geert , Speelman-Rooms Femke 
Published: December 2022

Abstract
Intracellular Ca2+ signals control several physiological and pathophysiological processes. The main tool to chelate intracellular Ca2+ is intracellular BAPTA (BAPTAi), usually introduced into cells as a membrane-permeant acetoxymethyl ester (BAPTA-AM). We previously demonstrated that BAPTAi enhanced apoptosis induced by venetoclax, a Bcl-2 antagonist, in diffuse large B-cell lymphoma (DLBCL). These findings implied a novel interplay between intracellular Ca2+ signaling and anti-apoptotic Bcl-2 function. Hence, we set out to identify the underlying mechanisms by which BAPTAi enhances cell death in B-cell cancers. In this study, we observed that BAPTAi alone induced apoptosis in lymphoma cell models that were highly sensitive to S63845, an Mcl-1 antagonist. BAPTAi provoked a rapid decline in Mcl-1 protein levels by inhibiting mTORC1-driven MCL-1 translation. Overexpression of nondegradable Mcl-1 rescued BAPTAi-induced cell death. We further examined how BAPTAi diminished mTORC1 activity and found that BAPTAi impaired glycolysis by directly inhibiting 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) activity, an up to now unkown effect of BAPTAi. All aforementioned effects of BAPTAi were also elicited by a BAPTAi analog with low affinity for Ca2+. Thus, our work reveals PFKFB3 inhibition as an unappreciated Ca2+-independent mechanism by which BAPTAi impairs cellular metabolism and ultimately the survival of Mcl-1-dependent cancer cells. Our work has two important implications. First, direct inhibition of PFKFB3 emerged as a key regulator of mTORC1 activity and a promising target in the treatment of Mcl-1-dependent cancers. Second, cellular effects caused by BAPTAi are not necessarily related to Ca2+ signaling. Our data support the need for a reassessment of the role of Ca2+ in cellular processes when findings were based on the use of BAPTAi.
Decoupling of land use net carbon flux, economic growth, and population change in China
Authors: Huang Xianke , Huang Yujie , Li Ruiliang , Cheng Wei , Su Yang , Li Feng , Du XianXiang 
Published: December 2022

Abstract
In the process of China’s modernization, promoting the sustainable development of resource-based cities is a major strategic issue and it has now also become a worldwide issue. This study uses the coupling model to validate the coupling relationship between China’s land-use net carbon flux and economic growth and population change during 2009–2017. The study for the first time draws the conclusion that the coupling degree among the three is getting lower, the correlation is gradually weaker, and the independent relationship is becoming more and more prominent. Utilizing the Tapio decoupling model, we obtained the weak decoupling conclusion that the economic growth rate is higher than the growth rate of the land-use net carbon flux, while negative decoupling of sprawl where the rate of population growth is less than the rate of net land use carbon flux growth.
Decadal Indian Ocean influence on the ENSO-Indian summer monsoon teleconnection is mostly apparent
Authors: Bódai Tamás , Aneesh Sundaresan , Lee June-Yi , Lee Sun-Seon 
Published: December 2022

Abstract
“Decadal influence” on the El Nin ̃o–Southern Oscillation-Indian summer monsoon (ENSO-ISM) teleconnection has been much studied but with plurality and ambiguity about the concept of influence. Here we provide formal definitions of the apparent influence of a specific factor on the teleconnection, as correlation coefficients, which enable us to test them as null-hypotheses. Using the recently released Community Earth System Model v2 Large Ensemble data, we show that a 50% chance for the detection of the apparent Indian Ocean influence under stationary conditions might take 2000 years of data. However, we find that this influence is mostly apparent indeed, as it originates from fluctuations of the decadal apparent moving window – as opposed to stationary climatological – ENSO variability, which causally influences an Indian Ocean Dipole-like apparent mean state. We also show that unattributed so-called “decadal influence”, defined as a deviation from a linear regression model of the teleconnection as a null-hypothesis, cannot be detected in 20th c. observations in South Asia even regionally. However, as a new insight, we find our new simple influence concept as a significant correlation coefficient to be possibly consistent with such a null-hypothesis.
The Role of Communities and Leadership in Ending Female Genital Mutilation in Tanzania: an Exploratory Cross-sectional Qualitative Study in Tanga
Authors: Mkuwa Serafina , Sempeho Jane , Kimbute Omari , Mushy Stella Emmanuel , Ndjovu Anthony , Mfaume Juhudi , Ngalesoni Frida 
Published: December 2022

Abstract
Background
Female genital mutilation (FGM) is one of the diehard cultures in the Mediterranean and sub-Saharan Africa. The act involves chopping off part of the female genitals in varying degrees depending on the society. The motive behind this practice includes reducing female sexual desire and as a sign of maturation. The current study explored the roles of community members and leaders in the fight against FGM; and the reasons for continuing the practice in some societies.
Method: We did an exploratory cross-sectional qualitative study between June – July 2020 in six purposively selected villages from Kilindi and Handeni districts in Tanga that were part of the ten years of implementation of the project. Ethnic leaders and village members aged 19 years and above were purposively selected. Due to the sensitive nature of the study, FGDs were conducted separately between men and women. In addition, we did the inductive thematic analysis.
Results: Four main themes emerged from the analysis; (1) history of FGM and reasons behind persistent FGM practices, (2) Challenges to abandonment of FGM, (3) strategies to be used to eradicate FGM, and (4) Key change agents in ending FGM. It was reported that the FGM practice was inherited from elders years ago and is believed to reduce women's sexual desire when the husband travels away for a long time. Some societies still practice FGM but secretly because for them marrying an uncircumcised girl is a curse as the husband and children will die. Some older women still practice FGM as they still hold the ancient culture. Constant communication with community leaders, seniors, and the young generation on complications of FGM will fasten efforts toward eradicating FGM practice.
Conclusion
There are sporadic cases done secretly associated with FGM practice since the ant-FGM campaign, so this should be the reason to continue with the campaign. Winning the tribal/ethnic leaders can facilitate better achievement in the fight against FGM. In addition, social diffusion with inter-tribe marriages was also singled out as one of the factors that will make FGM practice unfamiliar to the communities in the future.
Statistical analysis of the landslides triggered by the 2021 SW Chelgard earthquake (ML=6) using an automatic linear regression (LINEAR) and artificial neural network (ANN) model based on controlling parameters
Authors: Vanani Ali Asghar Ghaedi , Eslami Mohamad , Ghiasi Yusof , Keyvani Forooz 
Published: December 2022

Abstract

This study uses automatic linear regression (LINEAR) and artificial neural network (ANN) models to statistically analyze the area of landslides triggered by the 2021 SW Chelgard earthquake (ML = 6) based on controlling parameters. We recorded and mapped the number of 632 landslides into four groups (based on the Hungr et al. 2014): rock avalanche-rock fall, debris avalanche-flow, rock slump, and slide earth flow-soil slump using field observation, satellite images, and remote sensing method (before and after the earthquake). The results revealed that most landslides are related to debris avalanche-flow, rock avalanche, and slide earth flow under the disruption influence of slope structures in limestone and shale units and water absorption after the earthquake in young alluviums and terraces. The spatial distribution of landslides showed that the highest values of the landslide area percentage (LAP%) and of the landslide number density (LND, N/km2) occurred in the northern part of the fault on the hanging wall. The ANN models with R2 = 0.60-0.75 provided more accurate predictions of landslide area (LA, m2) than the LINEAR models, with R2 = 0.40-0.60 using multiple parameters. The elevation and slope were found to be the most influential parameters on the rock slump and the debris avalanche using ANN and LINEAR models. Aspect and elevation are the most important parameters for rock avalanches and rockfalls. The sliding earth flow and soil slump are most affected by the slope and elevation parameters. The peak ground acceleration (PGA) and the distance from the epicenter exhibited more effects on the LA than the intensity of Arias (Ia) and the distance from the rupture surface. Thus, the separation of seismic landslides using the classification of Hungr et al. (2014) can be helpful for predicting the LA more accurately and understanding the failure mechanism better.
State of E-Governance at Urban Local Government in Bangladesh: A Paradigm of Rajshahi City Corporation
Authors: Hasan Sabbir , Ullah S.M. Akram 
Published: December 2022

Abstract
The purport of this study is to depict the present scenario of e-governance at urban local government in Bangladesh. Rajshahi City Corporation (RCC), as a leading municipality in initiating and applying e-governance, has introduced almost ten e-services. After a deep exploration on RCC, it reveals that EBRIS and SARMS both the e-governance projections of RCC are successfully implemented presently. E-tender, e-nothi, e-death registration and web portals are also fairly implemented. And e-revenue and e-application are on the way to implementation. Contrarily, e-waste management, sms-based information service and smart rajshahi app are not implemented yet. The most realistic findings of this research are the unimproved ICT infrastructure, lack of proper IT training of the e-service providers, lack of cooperativeness of e-service receivers, lack of e-literacy of e-service receivers, intervention of third party and demanding bribe for providing e-services, harassment by e-service providers, lack of efficiency of e-service providers and lack of awareness of e-service receivers about e-governance. As an empirical research, it uses both the qualitative and quantitative methods. This study is an endeavor to present an overall current status of e-governance in RCC.
Flood Impact Assessment on Agricultural and Municipal Areas Using Sentinel-1 and 2 Satellite Images(Case study: Kermanshah province)
Authors: Gord Sadaf , Mavaddat Maryam Hafezparast , Ghobadian Rasoul 
Published: December 2022

Abstract

Flood is one of the most destructive natural events that can be investigated as soon as possible to reduce the extent of flood damage. Floods have caused adverse impacts on agricultural lands and settlements. RS is one of the most widely applicable and fast methods in this way. In this study, the S1A, SAR data with VH descending pass and S2 during 01/03/2019 to 20/03/2019 and 25/03/2019 to 20/04/2019 were used for the before and the post-flood period in Kermanshah province. MNDWI and NDWI were applied to detect the water zones of S2 and were compared and validate the S1 images. The quality of radar images is better than optical in flood conditions where the weather is cloudy and rainy. MODIS, Hydrosheds and SRTM satellite images were used as different filters to detect land use, permanent water and areas with slope of more than 5%. The results showed that totally 36,849 ha of lands are affected by the flood. It’s containing 7073 and 4224 ha of agricultural and urban lands that are exposed to destruction in this period. The NDWI and MNDWI indices were calculated flooded area as 30,179 and 32,540 ha, respectively which are lower values compared to the results of the S1.
Analysis of the Epidemiological Burden of intestinal obstruction in China 1990- 2019 Based on the Big Data of Global Burden of Disease Study (GBD)
Authors: Wu Junhong , Hou Zhibing , Chen Jiong , Zhao Hu , Zhang Zaizhong , Wang Yu , Lin Chengzhi 
Published: December 2022

Abstract
Background:A comprehensive assessment of the burden of intestinal obstruction was important in determining prevention strategies for the disease. We presented the trends in disease burden and its epidemiological features in China from 1990 to 2019 in order to provide a reference for the prevention of intestinal obstruction.
Methods: The GBD 2019 database was searched to obtain relevant data. The data we use included the prevalent cases, prevalence, incident cases, incidence, DALYs, DALY rate, and SDI for global and China. We analyzed the epidemiological age-period-cohort trend of intestinal obstruction in China from 1990 to 2019, and explored the relationship between the incidence and DALY rate and SDI.
Result: The incidence of intestinal obstruction in China increased in the last 30 years from 1990 to 2019, but the increase was not significant and remained stable in recent years. Among them, the incidence of 10-14 years youth group was the lowest, the incidence of 1-4 years infants’ group was higher, and the 85-89 years age group had the highest incidence, while the DALY rate decreased significantly with the obviously rise in SDI levels.
Conclusion: The disease burden of intestinal obstruction in China decreased to some extent from 1990 to 2019, which was negatively correlated with the level of social development. However, the prevention of intestinal obstruction in the elderly, infants and children still needs to be stepped up.
Natural infestation of an anchor worm, Lernaea sp. in cage culture of Asian Seabass, Lates calcarifer juveniles and its control using an anti-parasitic drug, emamectin benzoate
Authors: Ramalingam Ananda Raja , Patil Prasanna Kumar , Avunje Sathesha , Kumaran Mariappan , Anand Periyakaruppan , Kondusamy Ambasankar , De Debasis , Jithendran Karingalakkandy Poochirian , Alavandi Shankar Vinayakarao , Vijayan Koyadan Kizhakedath 
Published: December 2022

Abstract

Parasitic infestations and their control programmes are one among the challenges to be considered the most significant in aquaculture. A parasitic infestation was studied elaborately in Asian Seabass, Lates calcarifer juveniles with clinical signs, post-mortem findings, morphological and molecular identifications. In addition, those fish were also treated with emamectin benzoate (EMB) @ 50 µg kg-1 of fish body weight (BW) d-1 for 10 consecutive days under the controlled wet lab facility by feeding through the medicated feed at 4% BW. Results showed that the parasitic prevalence, parasitic intensity (PI) and mortality were 45.5%, 8.17 ± 0.15 per fish and 40% over a period of one week in that existing cage culture. The parasite was identified as a crustacean bloodsucker, anchor worm Lernaea sp. and EMB was found to be 100% effective with significant reduction in PI over a period of 10 days with improved survival rate of 90% against the untreated group. Infested but treated group revealed substantial haematological improvement in parameters such as RBC, WBC, Hb, PCV, large lymphocytes, small lymphocytes and total lymphocytes (P < 0.01). Similarly, comparative histopathology of vital organs also revealed no discernible lesions between the healthy and treated fish juvenile as compared to that of infested untreated group. Hence, EMB can be used to control the Lernaea sp. infestation in Asian Seabass.
The feasibility and acceptability of an app-based cognitive strategy training programme for older people.
Authors: Tulliani Nikki , Bye Rosalind , Bissett Michelle , Coutts Samantha , Liu Karen 
Published: December 2022

Abstract

Background: Increasing numbers of people are living with mild cognitive impairment in later life and seeking therapy to maintain cognition to remain as independent as possible in daily life. Based on a review of literature, an app-based programme using perceptual-encoding strategies called Enhancing Memory in Daily Life (E-MinD Life) was developed. An expert panel reviewed the programme’s appropriateness for older people with and without mild cognitive impairment. As part of the design process, the feasibility and acceptability of the E-MinD Life programme was then assessed in relation to its use by healthy older adults, with findings informing the application of the programme to older people with mild cognitive impairment in the future.
Methods: Phase One: The E-MinD Life programme was reviewed by an expert panel of occupational therapists. Experts rated the programme on a Likert scale and answered open-ended questions in relation to feasibility, clarity, and relevancy. Phase Two involved field-testing the 9-week programme with a sample of nine healthy older people. Participants rated the acceptability of the programme on a Likert scale questionnaire. Data on recruitment rates and retention, adherence and duration of sessions was collected to determine feasibility of the programme. Responses to the Likert scale were analysed using descriptive statistics. Open-ended responses were categorised qualitatively using a constant comparative approach.
Results: Phase One: Experts indicated that the E-MinD Life programme was feasible and included relevant activities for community living. Although experts felt that an older user with MCI would be able to independently complete the programme, the qualitative analysis suggests formatting changes in future iterations of the programme to enhance visual clarity.
Phase Two: All participants completed the nine-week programme. The average number of self-administered sessions attempted over the nine-week period was 13.44 (SD=6.73) out of 18 scheduled sessions. Overall, most participants found the programme relevant, logical and easy to understand, and perceived it to be effective for functional cognitive problems.
Conclusion: The E-MinD Life programme shows promise for the inclusion into trial designs to determine the effectiveness of the cognitive strategy programme for older people with and without cognitive impairment.
Trial registration: NCT03430401 Registered 1 February 2018, https://clinicaltrials.gov/ct2/show/NCT03430401?term=karen+liu&draw=2&rank=1
Determination of water quality in the Western Mediterranean River basin in Türkiye by using macrophyte indexes
Authors: Kızılırmaklı Aybüke , Cabi Evren , Demir Ogün , Turan Nesibe 
Published: December 2022

Abstract
In this study, we determined the macrophyte diversity, ecological quality based on Macrophyte Biological Index for Rivers (IBMR), and the correlations between macrophytes and physicochemical variables in 17 rivers in the Western Mediterranean Basin in Türkiye. In addition to these, we performed hierarchical clustering analysis to determine similarities of stations according to the macrophyte diversity and physicochemical variables. The IBMR values ranged between 6 and 12. Consequently, we found that the basin is at the mesotrophic-eutrophic level. The high levels of biological oxygen demand, total phosphorus, and suspended solids associated with organic and physical pollution in most stations and the frequency of the emergent taxa support the IBMR results and trophic level of rivers. Also, we found that water bodies chemically similar are relatively floristically similar.
Long-term humoral response following simultaneous Delta and Omicron BA.1 co-infection
Authors: Saade Carla , Pozzetto Bruno , Novoa Melyssa Yaugel , Josset Laurence , Lina Bruno , Paul Stéphane , Bal Antonin , Assant Sophie 
Published: December 2022

Abstract
To provide insight into the long-term immune response following bivalent vaccines, we sampled vaccinated patients simultaneously co-infected with Delta and BA.1. We reported that simultaneous exposure to the Delta and BA.1 S protein does not confer an additional immune advantage compared to exposure to the Omicron BA.1 S protein alone.
Insomniacs show greater prefrontal activation during verbal fluency task compared to non-insomniacs: A functional near-infrared spectroscopy investigation of depression in patients
Authors: Xu HuaSen , Wang YuXing , Wang Yi Ming , Cao YaQi , Li PeiFan , Hu YongXue , Xia GuangYuan 
Published: December 2022

Abstract
Background: Previous studies have shown that insomnia affects human prefrontal function and that there are specific patterns of brain activation to counteract sleep and improve cognition; however, the effects of insomnia on the prefrontal cortex of MMD patients and the patterns of activation to counteract sleep in MMD patients remain unclear. The aim of this study was to examine it using functional near infrared spectroscopy.
Methods: Eighty depressed patients and 44 healthy controls were recruited in this study.fNIRS was used to assess the changes in the concentration of oxygenated hemoglobin([oxy-Hb])in the prefrontal cortex of all Participants during a verbal fluency task(VFT) and record the number of words created to assess cognitive ability. The Pittsburgh sleep quality index assessed the sleeping quality. Hamilton Rating Scale for Depression 24 item and Hamilton Rating Scale for Anxiety 14 item were used to assess the Severity of depression and anxiety.
Results: Comparing patients, the healthy control group had significantly higher [oxy-Hb] values in the bilateral prefrontal cortex during VFT than the MMD group. In the MMD group, the [oxy-Hb] values in all brain regions except the right DLPFC were significantly lower in the group without insomnia than in the group with insomnia， their VFT performance was significantly lower than in the group without insomnia and the healthy group. PSQI scores were positively correlated with [oxy-Hb] values in some left-brain regions, whereas HAMD and HAMA scores were not correlated with [oxy-Hb] values.
Conslusion: The PFC brain region was significantly less active during VFT in those with MDD than in healthy controls. All brain regions, except the right DLPFC, were significantly more active in MMD patients with insomnia than in those without insomnia, suggesting that sleep quality needs to be an important indicator in fNIRS screening. In addition, there was a positive correlation between the severity of insomnia in the left VLPFC and the level of activation, suggesting a role for the left brain region in the neurophysiology of overcoming sleepiness in MMD patients; these findings may provide new ideas for the treatment of MMD patients in the future.
Trial registration: Our experiment was registered in the China Clinical Trial Registry (registration number ChiCTR2200065622) on November 10.( The first patient was recruited in 10/11/2022.)