bluuebunny/crossref_metadata_2025_split huggingface dataset:
Larval movement of Anticarsia gemmatalis (Lepidoptera: Erebidae) on vegetative and reproductive stage non-Bt soybean
Authors: Ongaratto Sabrina , Baldin Edson L. L. , Hunt Thomas E. , Luhr Nicole , Heier Kory R. , Robinson Emily A. 
Published: March 2022

Abstract
Integrated pest management (IPM) and insect resistance management (IRM) in a cropping system demands a comprehensive understanding of insect behavior, including larval movement. We evaluated the larval movement of velvetbean caterpillar, Anticarsia gemmatalis (Hübner), in a non-Bt soybean cultivar. Third to fifth instars were infested in different combinations in vegetative and reproductive soybean stages, and on-plant movement was characterized across select time points. The dispersion among plants in different positions and orientations was assessed by releasing 24-h-old larvae on the central plant of field plots. Results indicated that A. gemmatalis larvae are consistent in their movement during the day, regardless of plant section or leaf position. Over the time period of assessment, larvae gradually moved down and left the plant. Although there was not significant difference in the distance traveled by A. gemmatalis between orientations and positions, long distances were covered indicating that this species has nondirectional movement. This study provides relevant information regarding the movement of the velvetbean caterpillar, which can be used to improve insecticide spraying efficacy, improve probabilistic model predictive ability, and help design insect resistance management strategies.
Cooperative Boosting Performance of Halide Perovskite Solar Cells between Photoelectric and Thermoelectric Effect
Authors: Fu Ping , Lu Ruixue , Haque Md Azimul , You Enming , Li Hui , Chen Ruotian , Han Yaoyao , Yin Yanfeng , Qin Wei , Fan Fengtao , Wu Kaifeng , Baran Derya , Li Can 
Published: March 2022

Abstract
The sufficient utilization of longer-wavelength infrared light is a long-sought goal for efficient perovskite solar cells (PSCs) since they convert into heat easily and destroy the device stability. Establishing thermoelectric effect on the same PSCs is supposed to be an efficient way to convert heat into electricity. However, whether thermoelectric effect exists during the photovoltaic process has never been concerned. Herein, we report that the FAP-based PSCs operated under temperature differences (ΔT=15 oC) afford a champion PCE of 25.74%, higher than 22.84% with ΔT=0 oC, along with an improved Voc from 1.17 V to 1.23 V. It’s demonstrated that integrating photoelectric and thermoelectric effects can magnify the solar-to-electricity conversion. The critical factors to success are utilizing adequately photocarrier in parallel with regulating the intrinsic carrier directional transport and facilitating the charge carriers’ collection. The work shed light on the synergistic contribution of photoelectric and thermoelectric effect to efficient PSCs.
Electric cookstove adoption and energy attitudes in rural Namibia: The role of youth and sustainability education in advancing household energy
Authors: Lindgren Samantha 
Published: March 2022

Abstract
Efficient cookstoves are popular solutions in international energy development for their presence in multiple Sustainable Development Goals. However, adoption of these devices remains a challenge. Among the barriers known to limit adoption is effective household communication, yet rarely are all stakeholders purposefully engaged in activities that support uptake and sustained use. The findings of two studies conducted in rural Namibia indicate that youth-oriented sustainability education is an effective communication tool in cooking energy development, and has the potential to shift attitudes and behaviours, within households and across communities. Households with children who participated in sustainability education programming were significantly more likely to prefer efficient fuels and be less committed to biomass cookstoves than similar families without such children. Further, a longitudinal study of children who attended the programming indicates that their preferences about common cooking fuels shifted toward sustainable ones, and that these changes persist over time.
Incidence of Leber hereditary optic neuropathy in 2019 in Japan: A second nationwide questionnaire survey
Authors: Takano Fumio , Ueda Kaori , Godefrooij Daniel A. , Yamagami Akiko , Ishikawa Hiroto , Chuman Hideki , Ishikawa Hitoshi , Ikeda Yasuhiro , Sakamoto Taiji , Nakamura Makoto 
Published: March 2022

Abstract
Background: Leber hereditary optic neuropathy (LHON) is an acute or subacute optic neuropathy that mainly affects young males. The first nationwide epidemiological survey of LHON was conducted in 2014 in Japan, and LHON was officially designated as a rare intractable disease by the Japanese government in 2015. We conducted a second survey of the annual incidence of LHON in 2019, and estimated the total number of patients with LHON in Japan.Results: A questionnaire was sent to 997 facilities accredited by the Japanese Ophthalmological Society and/or affiliated with the councilors of the Japanese Neuro-Ophthalmology Society. Responses were received from 791 facilities, with a recovery rate of 79%. Fifty-six new cases (49 males and 6 females) of LHON were reported from 35 institutions in 2019, with a median age of 28.5 for males and 49.5 years for females. The total number of new cases was calculated as 69 (62 were males and 7 were females, 95% confidence interval 55–83), and the total number of patients was estimated to be 2,491 (95% confidence interval: 1996–2986), suggesting a prevalence of LHON in Japan of 1:50000. Conclusion: The incidence of LHON in 2019 was lower than the estimate in 2014, whereas its prevalence may be similar to that reported in other countries. The accurate estimation of the incidence and prevalence of patients with LHON requires prospective registration.
Effect of referral by community health workers on uptake of intermittent preventive treatment of malaria in pregnancy in Ohaukwu, Ebonyi State Nigeria: An analysis of individual level data from the maternity record booklet.
Authors: Orji Bright , Bryce Emily , Odio Bartholomew , Onuoha Herbert , Njoku Elizabeth , Anoke Charity , Ugwa Emmanuel , Enne Joseph , Oniyire Adetiloye , Otolorin Emmanuel , Afolabi Kayode , Ogbulafor Nnenna C. , Oliveras Elizabeth 
Published: March 2022

Abstract
Background: The World Health Organization recommends a minimum of three doses of quality-assured sulfadoxine-pyrimethamine for intermittent preventive treatment of malaria during pregnancy (IPTp), in moderate to high malaria transmission areas in sub-Saharan Africa. This treatment prevents malaria in pregnancy contributing to reduced maternal and infant morbidity and mortality. Currently, coverage of IPTp lags behind coverage of antenatal care (ANC) visits; in Nigeria, 57% of women attended four or more ANC visits, whereas only 17% received the recommended three or more doses of IPTp. The innovative program aimed to close this gap by providing counseling on the benefits of comprehensive ANC, referral to ANC and community distribution of IPTp (C-IPTp), complementing IPTp at ANC. Methods: The data for this study were extracted from the maternity record books for pregnant women at 25 public health facilities in Ebonyi State, Nigeria. The outcome of interest was defined as the receipt of three or more doses of IPTp (IPTp3) and the primary covariate was referral to ANC by a community health worker for any visit. Descriptive statistics were reported and the results from the multi-level regressions are reported as adjusted odds and prevalence ratios with corresponding 95% confidence intervals. Results: Of the 936 women included in the analysis, 24.47% received the recommended three or more IPTp doses and 61.32% were referred by a community health worker (CHW) for at least one ANC visit. There was no difference in the average number of ANC visits between women who received C-IPTp and those who received IPTp exclusively at a facility. There were no maternal characteristics associated with CHW referral. Women who were referred by a CHW had 60% greater odds (95% CI: 1.08-2.38) of receiving IPTp3 than those who were never referred. Conclusion: The results indicate that CHW referrals conducted within a C-IPTp program increase IPTp uptake without disrupting ANC attendance and that CHWs applied the referral process equally. This strengthens the evidence base for C-IPTp scale-up, which could have a large impact in sub-Saharan Africa in mitigating existing health systems issues.
Quality control recommendations for RNASeq using FFPE samples based on pre-sequencing lab metrics and post-sequencing bioinformatics metrics
Authors: Liu Yuanhang , Bhagwate Aditya , Winham Stacey J , Stephens Melissa T , Harker Brent W , McDonough Samantha J , Stallings-Mann Melody L , Heinzen Ethan P , Vierkant Robert A , Hoskin Tanya L , Frost Marlene , Carter Jodi M , Pfrender Michael E. , Littlepage Laurie , Radisky Derek C , Cunningham Julie M , Degnim Amy C , Wang Chen 
Published: March 2022

Abstract
BackgroundFormalin-fixed, paraffin-embedded (FFPE) tissues have many advantages for identification of risk biomarkers, including wide availability and potential for extended follow-up endpoints. However, RNA derived from archival FFPE samples has limited quality. Here we identified parameters that determine which FFPE samples have the potential for successful RNA extraction, library preparation, and generation of usable RNAseq data.MethodsWe optimized library preparation protocols designed for use with FFPE samples using seven FFPE and Fresh Frozen replicate pairs, and tested optimized protocols using a study set of 130 FFPE biopsies from women with benign breast disease. Metrics from RNA extraction and preparation procedures were collected and compared with bioinformatics sequencing summary statistics. Finally, a decision tree model was built to learn the relationship between pre-sequencing lab metrics and qc pass/fail status as determined by bioinformatics metrics.ResultsSamples that failed bioinformatics qc tended to have low median sample-wise correlation within the cohort (Spearman correlation < 0.75), low number of reads mapped to gene regions (< 25 million), or low number of detectable genes (11,400 # of detected genes with CPM > 2). The median RNA concentration and pre-capture library Qubit values for qc failed samples were 18.7 ng/ul and 1.68 ng/ul respectively, which were significantly lower than those of qc pass samples (40.8 ng/ul and 5.58 ng/ul). We built a decision tree model based on input RNA concentration, input library qubit values, and achieved an F score of 0.803 in predicting QC status (pass/fail) of FFPE samples. ConclusionsWe provide a bioinformatics quality control recommendation for FFPE samples from breast tissue by evaluating bioinformatic and sample metrics. Our results suggest a minimum concentration of 25 ng/ul FFPE-extracted RNA for library preparation and 1.8 ng/ul pre-capture library output to achieve adequate RNA-seq data for downstream bioinformatics analysis.
Responses and tolerance mechanisms of K. paniculate seedlings under lead stress: physiological indicators, morphological distribution, enrichment effects and microstructure
Authors: Chen Yonghua , Xie Tianzhi , Su Rongkui , Yao Haisong , Du Lu , Liu Jun 
Published: March 2022

Abstract
Phytoremediation could be an alternative strategy for lead (Pb) contamination, The physiological mechanisms of plant resistance to Pd contamination are of less concern. In this study, Different concentrations of Pb solutions were added to explore the growth of Koelreuteria paniculate (Sapindaceae). physiology and microstructure of K. paniculate were determined. The results showed that plant growth increased up to 123.8% and 112.7% relative to the control group when the lead concentration was 200 and 400 mg/L. In all treatments, most of the Pb accumulation reached 81.31%-86.69% in the roots and slightly lower concentrations in the above-ground leaves than in the stems. Transmission electron microscopy (TEM) showed that Pb accumulates in the inactive metabolic parts (cell walls and vesicles) in roots and stems, which may be the main mechanism for plants to reduce Pb biotoxicity. In addition, by Fourier transform infrared spectroscopy (FTIR), Pb stress increased the content of intracellular -OH and -COOH functional groups, including compounds such as organic acids, polysaccharides and proteins that bind to Pb, attenuating the toxicity to plants and enhancing their adaptation and tolerance to heavy metals.
Two SKAP2 modular organizations differently recognize SRC kinases depending on their activation status and localization
Authors: Levillayer Laurine , Cassonnet Patricia , Declercq Marion , Santos Mélanie Dos , Lebreton Louis , Danezi Katerina , Demeret Caroline , Sakuntabhai Anavaj , Jacob Yves , Bureau Jean-François 
Published: March 2022

Abstract
Background: The activation of SRC kinases by trans-phosphorylation raises the question of their interplay. This work addresses this question by studying the interactions of SRC kinases with the SRC Kinase Adaptor Phosphoprotein 2 (SKAP2) by combining a luciferase complementation assay and extensive site-directed mutagenesis. Results: SKAP2 interacts with SRC kinases through two different module organizations that have different affinities for the activated and non-activated kinase conformations. The DIM domain of SKAP2 is necessary and sufficient to bind to most activated SRC kinases. A second SKAP2 module organization for binding to SRC kinases at their steady state is composed of the DIM and SH3 domains as well as the linker located between the PH and the SH3 domains. Classical binding sites of the PH and SH3 domains and the tyrosines located inside linkers modulate these interactions. Analysis of HCK mutants supports this model and shows the role of two residues, Y390 and K7, on its degradation following activation. Conclusion: In this article, we show that SKAP2 have a modular architecture composed of 2 domains and one interdomain to interact with SRC kinases, which binding capacity of each module depends on their localization and activation. This work opens new perspectives on how activation of SRC kinases occurs, which could have new and interesting therapeutic consequences.
The Impact of Covid-19 Pandemic on Child Abuse and Neglect
Authors: Lupariello Francesco , Mattioda Giuliana , Lauria Barbara , Aguzzi Sonia , Castagno Emanuele , Bondone Claudia , Vella Giancarlo Di 
Published: March 2022

Abstract
Purpose - Child abuse and neglect (CAN) is a worldwide issue due to its biological, psychological, and social effects. Research in this field is particularly useful to understand CAN aspects which are still unknown and to define updated intervention strategies. Until now, there are no clear indications about the possible effects determined by the SARS-CoV-2 pandemic on CAN. For these reasons, the authors of the present manuscript propose a study in which they compared quantitative and qualitative variables of pre-pandemic and pandemic cases evaluated at an Italian ambulatory specialized in diagnosis and management of CAN. The main aims were to: define if there was a reduction or an increase of cases referred for CAN during SARS-CoV-2 pandemic; evaluate if there were statistically significant differences of variables’ distributions between pre-pandemic and pandemic periods. Methods - The study included children 0 - 18 years of age who were referred to the specialized ambulatory during the first 13 months of the SARS-COV-2 pandemic in Italy: March 2020 - March 2021. As control group (Group B - GB), the authors chose children 0 - 18 years of age evaluated by the healthcare professionals of the same ambulatory from March 2018 to March 2019. Ages and main qualitative variables were compared between the two samples throughout statistical tests. Results - The analysis yielded no statistical differences for age distributions and for the most part of qualitative variables. The study pointed out a reduction of the distribution of the number of cases. Conclusions - In this study, the comparison of frequencies’ distribution of the most part of variables between pre and post pandemic samples did not differ. The most important result of the present analysis is that in the pandemic period the volume of observed CAN cases decreased. This allows us to confirm the indications of the scientific literature. We cannot exclude that the decrease was due to a negative impact of the pandemic on the child protection system. The afore-mentioned data will be useful to program corrective interventions, also for future pandemic and disasters.
Alterations in Mitochondria-Associated Endoplasmic Reticulum Membranes Under Oxidative Stress in R28 Cells
Authors: Yang Yuting , Wu Jihong , Lu Wei , Dai Yiqin , Zhang Youjia , Sun Xinghuai 
Published: March 2022

Abstract
This study aimed to investigate alterations in mitochondria-associated endoplasmic reticulum membranes (MAMs) under oxidative stress conditions in R28 retinal precursor cells. Hydrogen peroxide (H2O2) and hypoxia (0.2% oxygen) were used to induce oxidative stress in R28 cells. Cell viability, reactive oxygen species (ROS) levels, mitochondrial membrane potential, mitochondrial calcium concentration, MAMs, and autophagy were evaluated. Mitofusin-2 (MFN2), a protein that plays a role in the structural regulation of MAMs, was either upregulated or downregulated in R28 cells to further explore the potential role of MFN2 in modulating MAMs. Oxidative stress not only modulated MAMs but also induced mitochondrial membrane potential (MMP) and increased mitochondrial calcium concentration. H2O2 and hypoxia also induced oxidative stress-associated autophagy in R28 cells. Additionally, autophagy inhibition increased MAM signaling. MFN2 silencing increased MAM expression and induced MMP collapse under normal conditions, whereas MFN2 upregulation rescued hypoxia-induced MAM alterations and alleviated MMP collapse. However, Mfn2 overexpression further enhanced H2O2-induced MAMs and failed to rescue MMP collapse. Our study indicates that MAMs possibly serve as a membrane source for oxidative stress-associated autophagy in R28 cells. MFN2 appears to play a negative role in maintaining MAM biogenesis under hypoxic conditions; however, its role in H2O2-associated MAM dysfunction remains unclear.
A Nonextensive View on the COVID-19 in the World
Authors: Liu Yang , Yu Chen-Yue , Shen Ke-Ming 
Published: March 2022

Abstract
This paper deals with a general Susceptible-Infectious-Removed (SIR) model for the coronavirus disease 2019 (COVID-19) within a non-extensive view. A time-dependent SIR model was modified when particularly regarding control and mitigation measures in response to the societal impacts of epidemics and pandemics. We validated all the theoretical results by fitting the derived q-distributions with data from the COVID-19 pandemic in the world. It was found that not all the changeable fit parameters are independent, some of which shared common properties, a result corroborated by our model prediction. Our modified SIR model was proved to be effective in fitting the COVID-19 epidemic distributions. The relative non-extensive parameter was strongly connected with the freedom of systems, which thus threw a light upon the prevention and treatment of disease next in the world.
Deep Learning for MYC binding site recognition
Authors: Fioresi R. , Demurtas P. , Perini G. 
Published: March 2022

Abstract
Motivation: The definition of the genome distribution of the Myc transcription factor is extremely important since it may help predict its transcriptional activity particularly in the context of cancer. Myc is among the most powerful oncogenes involved in the occurrence and development of more than 80% of different types of pediatric and adult cancers. Myc regulates thousands of genes which can be in part different, depending on the type of tissues and tumours. Myc distribution along the genome has been determined experimentally through chromatin immunoprecipitation This approach, although powerful, is very time consuming and cannot be routinely applied to tumours of individual patients. Thus, it becomes of paramount importance to develop in-silico tools that can effectively and rapidly predict its distribution on a given cell genome. New advanced computational tools (DeeperBind) can then be successfully employed to determine the function of Myc in a specific tumour, and may help to devise new directions and approaches to experiments first and personalized and more effective therapeutic treatments for a single patient later on. Results: The use of DeeperBind with DeepRAM on Colab platform can effectively predict the binding sites for the MYC factor with an accuracy above 0.96 AUC, when trained with multiple cell lines. The analysis of the filters in DeeperBind trained models shows, besides the consensus sequence CACGTG classically associated to the MYC factor, also the other consensus sequences G/C box or TGGGA, respectively bound by the SP1 and MIZ-1 transcription factors, which are known to mediate the MYC repressive response. Overall, our findings suggest a stronger sinergy between the machine learning tools as DeeperBind and biological experiments, which may reduce the time consuming experiments by providing a direction to guide them.
Activation of Swell1 in microglia suppresses neuroinflammation and reduces brain damage in ischemic stroke
Authors: Chen Baoyi , Xie Cong , Shi Tengrui , Yue Shiqin , Li Weiping , Huang Guodong , Zhang Yuan , Liu Wenlan 
Published: March 2022

Abstract
Background
Neuroinflammation and immune responses mediated by microglia are associated with brain injury after ischemic stroke, and it is novel that Cl− movement and Cl-sensitive signal pathways have been shown to be implicated in the survival and switch of inflammation patterns of immune cells. Swell1, one of the components of Volume-regulated Cl− currents (VRAC) is considered to be the main channels mediating Cl− efflux from nerves cells. However, the role of Swell11 in ischemia-induced inflammation and the effect of Swell1 on the inflammation patterns switch of microglia remain unknown.
Methods
Whole-cell patch clamp recordings was used to examine Swell1-mediated VRAC in BV2 cells. In vivo and vitro studies comparing EGFP and Swell1 conditional knock-in mice were performed to determine the role of microglia Swell1 on inflammation and brain injury after ischemic stroke. In vitro experiments to further explored the mechanism of microglia Swell1 in regulating neuroinflammation.
Results
Our data showed that knockdown or overexpression of Swell1 in cultured BV2 cells inhibited or increased hypotonic-activated Cl− currents, respectively, and these changes were completely blocked by the VRAC inhibitor DCPIB. Swell1 conditional knock-in mice promoted microglial survival in the lesion region, leading to reductions in neural cell apoptosis, infarction size and neurological deficits following transient middle cerebral artery occlusion (tMCAO). Using gene manipulating technique and pharmacological inhibitors, we further revealed that Swell1 opening led to SGK1 (a Cl−-sensitive kinase)-mediated activation of FOXO3a/CREB as well as WNK1 (another Cl−-sensitive kinase)-mediated SPAK/OSR1-CCCs activation, which promoted microglial survival and M2 polarization, thereby attenuating inflammation and ischemic brain injury.
Conclusion
Our results demonstrated that Swell1 is an essential component of microglia VRAC and its activation protects against ischemic brain injury through promoting microglial survival and M2 polarization, which provides new a new orientation for the treatment of ischemic stroke and other neurological diseases associated with inflammation.