bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gambaran Asupan Makanan dan Status Gizi pada Ibu Hamil di Huntara Kelurahan Petobo Kota Palu
Authors: Kusumawati Dwi Erma , Nurwidianti Nurwidianti , Hafid Fahmi 
Published: June 2022

Introduction: Sufficient nutrient intake is vital for fetal growth and development during pregnancy. Stable food security supports adequate food consumption. Natural disaster that struck Palu City in 2018 had a negative influence on food security. Food intake and nutritional status among pregnant women in temporary housing in Petobo sub-district, South Palu sub-district are described in this study. Methods: The research method used in this study is descriptive. All pregnant women in temporary shelters were included in the population and samples, which totally 30 pregnant women. Data collected including MUAC and Food Recall for 24 hours over two non-consecutive days. The study results showed that the average intake was 1,370 kcal of energy, 232.7 grams of carbohydrates, 38.6 grams of protein, and 28.4 grams of fat. The majority for energy and macronutrient consumption are below 80% of the Indonesian RDA 2019. The average of Vitamin A 575.6 mcg, vitamin C 33.3 mg, vitamin B6 0.9 mg, calcium 679.5 mg, and zinc 4.2 mg are below 80% of the Indonesian RDA 2019. Only intake of Vitamin B9 and Iron is sufficient with an average intake of 511.3 mcg and 42.3 mg. The nutritional status of all pregnant women are not at risk of CED. In conclusion, intake of calories, macronutrient, and micronutrient are classified as inadequate except for vitamin B9 and Iron, and all pregnant women is not at risk of CED. It is suggested the diversify of diet by eating more locally grown foods and doing enough exercise.

ABSTRAK
Pendahuluan: Asupan zat gizi pada saat ibu hamil di perlukan untuk pertumbuhan dan perkembangan janin. Asupan makanan yang cukup di dukung adanya kestabilan ketahanan pangan. Bencana alam yang terjadi di kota palu pada tahun 2018 berdampak pada ketidakstabilan ketahanan pangan. penelitian ini menggambarkan asupan makanan dan status gizi pada ibu hamil di hunian sementara kelurahan Petobo kecamatan Palu Selatan. Metode: Penelitian ini menggunakan jenis penelitian deskriptif. Populasi dan sampel adalah semua ibu hamil yang berada di hunian sementara berjumlah 30 ibu hamil. Pengumpulan data berupa LILA dan Recal 24 jam selama 2 hari tidak berurutan. Hasil penelitian menunjukkan rata-rata asupan energi 1.370 kkal, karbohidrat 232,7 gr, protein 38,6 gr dan lemak 28,4 gr. sebagian besar asupan energi dan zat gizi makro masih <80% dari AKG. Asupan vitamin A 575,6 mcg, vitamin C 33,3 mg, vitamin B6 0,9 mg, kalsium 679,5 mg dan zink 4,2 mg masih < 80% dari AKG. Hanya asupan Vitamin B9 dan fe cukup dengan rerata asupan 511,3 mcg dan 42,3 mg serta Status gizi semua ibu hamil tidak berisiko KEK. Kesimpulan bahwa semua asupan energi, zat gizi makro dan sebagian besar dari zat gizi mikro berkategori kurang. Asupan vitamin B9 dan Fe ibu hamil cukup dengan status gizi semua ibu hamil tidak berisiko KEK. Disarankan untuk tingkatkan keanekaragaman makanan dari pangan lokal dan lakukan aktivitas fisik yang cukup.
The Difference of Gut Microbiota and Their Correlations With Urinary Organic Acids Between Autistic Children With and Without Atopic Dermatitis
Authors: Hong Ru-ping , Hou Yue-ying , Xu Xin-jie , Lang Ji-dong , Jin Yun-feng , Zeng Xiao-feng , Zhang Xuan , Tian Geng , You Xin 
Published: June 2022

Autism is a kind of biologically based neurodevelopmental condition, and the coexistence of atopic dermatitis (AD) is not uncommon. Given that the gut microbiota plays an important role in the development of both diseases, we aimed to explore the differences of gut microbiota and their correlations with urinary organic acids between autistic children with and without AD. We enrolled 61 autistic children including 36 with AD and 25 without AD. The gut microbiota was sequenced by metagenomic shotgun sequencing, and the diversity, compositions, and functional pathways were analyzed further. Urinary organic acids were assayed by gas chromatography–mass spectrometry, and univariate/multivariate analyses were applied. Spearman correlation analysis was conducted to explore their relationships. In our study, AD individuals had more prominent gastrointestinal disorders. The alpha diversity of the gut microbiota was lower in the AD group. LEfSe analysis showed a higher abundance of Anaerostipes caccae, Eubacterium hallii, and Bifidobacterium bifidum in AD individuals, with Akkermansia muciniphila, Roseburia intestinalis, Haemophilus parainfluenzae, and Rothia mucilaginosa in controls. Meanwhile, functional profiles showed that the pathway of lipid metabolism had a higher proportion in the AD group, and the pathway of xenobiotics biodegradation was abundant in controls. Among urinary organic acids, adipic acid, 3-hydroxyglutaric acid, tartaric acid, homovanillic acid, 2-hydroxyphenylacetic acid, aconitic acid, and 2-hydroxyhippuric acid were richer in the AD group. However, only adipic acid remained significant in the multivariate analysis (OR = 1.513, 95% CI [1.042, 2.198], P = 0.030). In the correlation analysis, Roseburia intestinalis had a negative correlation with aconitic acid (r = -0.14, P = 0.02), and the latter was positively correlated with adipic acid (r = 0.41, P = 0.006). Besides, the pathway of xenobiotics biodegradation seems to inversely correlate with adipic acid (r = -0.42, P = 0.18). The gut microbiota plays an important role in the development of AD in autistic children, and more well-designed studies are warranted to explore the underlying mechanism.
Long-Term Mortality After TAVI for Bicuspid vs. Tricuspid Aortic Stenosis: A Propensity-Matched Multicentre Cohort Study
Authors: Gasecka Aleksandra , Walczewski Michał , Witkowski Adam , Dabrowski Maciej , Huczek Zenon , Wilimski Radosław , Ochała Andrzej , Parma Radosław , Scisło Piotr , Rymuza Bartosz , Zbroński Karol , Szwed Piotr , Grygier Marek , Olasińska-Wiśniewska Anna , Jagielak Dariusz , Targoński Radosław , Opolski Grzegorz , Kochman Janusz 
Published: June 2022

ObjectivesPatients with bicuspid aortic valve (BAV) stenosis were excluded from the pivotal trials of transcatheter aortic valve implantation (TAVI). We compared the in-hospital and long-term outcomes between patients undergoing TAVI for bicuspid and tricuspid aortic valve (TAV) stenosis.MethodsWe performed a retrospective registry-based analysis on patients who underwent TAVI for BAV and TAV at five different centers between January 2009 and August 2017. The primary outcome was long-term all-cause mortality. Secondary outcomes were in-hospital mortality, procedural complications, and valve performance.ResultsOf 1,451 consecutive patients who underwent TAVI, two propensity-matched cohorts consisting of 130 patients with BAV and 390 patients with TAV were analyzed. All-cause mortality was comparable in both groups up to 10 years following TAVI (HR 1.09, 95% CI: 0.77–1.51). Device success and in-hospital mortality were comparable between the groups (96 vs. 95%, p = 0.554 and 2.3 vs. 2.1%, p = 0.863, respectively). Incidence of procedural complications was similar in both groups, with a trend toward a higher rate of stroke in patients with BAV (5 vs. 2%, p = 0.078). Incidence of moderate or severe paravalvular leak (PVL) at discharge was comparable in both groups (2 vs. 2%, p = 0.846). Among patients with BAV, all-cause mortality was similar in self-expanding and balloon-expandable prostheses (HR 1.02, 95% CI: 0.52–1.99) and lower in new-generation devices compared to old-generation valves (HR 0.27, 95% CI 0.12–0.62).ConclusionPatients who had undergone TAVI for BAV had comparable mortality to patients with TAV up to 10 years after the procedure. The device success, in-hospital mortality, procedural complications, and PVL rate were comparable between the groups. The high rate of neurological complications (5%) in patients with BAV warrants further investigation.
Can Outcomes of a Chat-Based Suicide Prevention Helpline Be Improved by Training Counselors in Motivational Interviewing? A Non-randomized Controlled Trial
Authors: Janssen Wilco , Raak Jeroen van , Lucht Yannick van der , Ballegooijen Wouter van , Mérelle Saskia 
Published: June 2022

ObjectiveTo examine whether the outcomes of a chat-based suicide-prevention helpline could be improved by training counselors in motivational interviewing (MI).MethodsIn a pre- and post-test design, visitors of a chat-based suicide prevention helpline received either the Five-Phase Model (treatment as usual [TAU]) or MI. They completed a pre- and post-chat questionnaire on several suicide-related risk factors. Linear mixed modeling was used to estimate the effect of the condition. Furthermore, the treatment proficiency of newly trained counselors was assessed using MI-Scope.ResultsA total of 756 visitors and 55 counselors were included in this study. The visitors showed an improvement in suicidal ideation and psychological risk factors after a chat conversation. However, there were no significant differences between the MI and TAU conditions (β = 0.03, 95% CI [−0.23–0.30], p = 0.80). The treatment integrity indices showed that the counselors mostly used MI-consistent techniques but were unable to strategically employ these techniques to evoke enough change talk.ConclusionsMI and TAU led to comparable outcomes in a chat-based suicide prevention helpline. The effectiveness of MI might improve by intensifying or improving the training of counselors, keeping the process of engaging more concise or offering visitors multiple sessions of MI.
Evolution Characteristics and Mechanism of the Load/Unload Response Ratio Based on Strain Observation Before the Jiuzhaigou MS7.0 Earthquake
Authors: Yue Chong , Ji Ping , Wang Yali , Yu Huaizhong , Cui Jin , Yu Chen , Ma Yuchuan 
Published: June 2022

Strain observation is the most intuitive observation equipment to monitor stress change in the crust. Strain equipment near the epicenter area of the Jiuzhaigou earthquake (JZGEQ) provides a lot of reliable pre-seismic and post-seismic data for the study of stress change. In this study, the load/unload response ratio (LURR) method is used to study the stress state of rocks by calculating the ratio of strain observation during the loading phase and unloading phase. Results show that the LURR method based on strain observation is an effective method to describe the dynamic change of the constitutive relationship of the rocks in the crust. Different from the strain observation, the LURR anomaly evolution process is more continuous regardless of the time series curve or spatial and temporal distribution characteristics. The LURR curves of different stations begin to increase above 1.0 gradually from 6 months to 1 year prior to the occurrence of the JZGEQ, reaching the maximum value in 1–3 months before the JZGEQ, and subsequently return to a low level. The maximum value of the LURR anomalies decreases with the distance from the epicenters. At the same time, the spatial and temporal evolution characteristics of the LURR anomalies show us the process of “extension—enhance—weaken” in the epicenter of JZGEQ and its peripheral area prior to the earthquake. The concentration areas of the aforementioned LURR anomalies are all distributed in the pre-seismic normal stress-loading zone, which indicates that the faults are in the process of decoupling, and microfracture may exist in the stage of rock dilatancy.
Hypovitaminosis D Is Prevalent in Patients With Renal AL Amyloidosis and Associated With Renal Outcome
Authors: Muchtar Eli , Drake Matthew T. , Leung Nelson , Dispenzieri Angela , Lacy Martha Q. , Buadi Francis K. , Dingli David , Hayman Suzanne R. , Kapoor Prashant , Hwa Yi Lisa , Fonder Amie , Hobbs Miriam , Gonsalves Wilson , Kourelis Taxiarchis V. , Warsame Rahma , Russell Stephen , Go Ronald S. , Binder Moritz , Kyle Robert A. , Rajkumar S. Vincent , Kumar Shaji K. , Gertz Morie A. 
Published: June 2022

IntroductionVitamin D deficiency is common, but no data have been reported on vitamin D levels in light chain (AL) amyloidosis.Patients and MethodsIn this exploratory study, stored serum samples from 173 patients with newly diagnosed AL amyloidosis were analyzed for vitamin studies which included 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D [1,25(OH)2D] and vitamin D binding protein (DBP). Measurements were made by liquid chromatography-tandem mass spectrometry. Kidney survival and overall survival (OS) were assessed in association to vitamin D status.ResultsCardiac and kidney involvement occurred in 69% and 63% of patients, respectively. 25(OH)D deficiency (&lt;20 ng/mL) was seen in 56.6% of the patients and was notably found among patients with heavy proteinuria (96%), hypoalbuminemia (84.3%) and morbidly obese patients (68.3%). Heavy proteinuria (&gt;5 gr/24-h) and vitamin D supplementation were independent predictors of 25(OH)D level on nominal multivariate regression analysis. 1,25(0H)2D deficiency was noted in 37.6% of patients and was independently associated with low eGFR and hypoalbuminemia. Progression to ESRD occurred in 23.7% of evaluable patients. Patients who progressed to ESRD had lower serum 25(OH)D and 1,25(OH)2D levels compared to those who did not progress to ESRD. On a multivariate analysis, severe 25(OH)D deficiency was an independent predictor of progression to ESRD as was renal stage, while 1,25(OH)2D deficiency was not.ConclusionsHypovitaminosis D is common in AL amyloidosis, particularly among patients with heavy proteinuria. Severe 25(OH)D deficiency at time of diagnosis predicts progression to ESRD.
Comparative Study of Impacts of Typical Bio-Inspired Optimization Algorithms on Source Inversion Performance
Authors: Mao Shushuai , Hu Feng , Lang Jianlei , Chen Tian , Cheng Shuiyuan 
Published: June 2022

Accurate identification of source information (i.e., source strength and location) is crucial for the air pollution control or effective accidental response. Optimization inversion based on bio-inspired algorithms (BIOs) is an effective method for estimating source information. However, the impacts of different BIOs and the shared parameter of population size in BIOs on source inversion performance have not been revealed. Here the source inversion performance (i.e., accuracy and robustness) of six typical BIOs [i.e., bacterial foraging optimization algorithm (BFO), chicken swarm optimization algorithm (CSO), differential evolution algorithm (DE), genetic algorithm (GA), particle swarm optimization (PSO), and seeker optimization algorithm (SOA)], and their population sizes are evaluated based on the Prairie Grass dataset which covering different atmospheric conditions (i.e., Pasquill stability classes A, B, C, D, E, and F). Results indicated the population size has substantial influence on source inversion. The accuracy of all BIOs in source strength fluctuated greatly when the population size was small, whereas, tended to be stable as the population size increased. Overall, the BFO had the best accuracy with lowest deviations (74.5% for source strength and 29.7 m for location parameter x0), whereas SOA had the best robustness for all source parameters. Atmospheric conditions indicated an obvious influence on the inversion performance of the BIOs. The BFO and CSO performed the best with the lowest deviations [137.5 and 26.7% for unstable conditions (A, B, and C) and stable condition (E), respectively], all algorithms are comparable (67.4 ± 2.1%) in neutral condition (D), and BFO and CSO had the comparable performances (23.2 and 24.3%) and performed better under extremely stable condition (F). This study enhances the understanding of the factors inﬂuencing source inversion and provides a reference for the selection of appropriate bio-inspired algorithms and the reasonable setting of population size parameter for source inversion in practical environmental management.
Revealing the Satellite DNA History in Psalidodon and Astyanax Characid Fish by Comparative Satellitomics
Authors: Goes Caio Augusto Gomes , dos Santos Rodrigo Zeni , Aguiar Weidy Rozendo Clemente , Alves Dálete Cássia Vieira , Silva Duílio Mazzoni Zerbinato de Andrade , Foresti Fausto , Oliveira Claudio , Utsunomia Ricardo , Porto-Foresti Fabio 
Published: June 2022

Eukaryotic genomes are usually enriched in repetitive DNA sequences, which can be classified as dispersed or tandemly repeated elements. Satellite DNAs are noncoding monomeric sequences organized in a head-to-tail fashion that are generally located on the subtelomeric and/or pericentromeric heterochromatin. In general, a single species incorporates a diverse group of satellite DNA families, which collection is called satellitome. Here, we characterized three new satellitomes from distinct characid fish (Psalidodon fasciatus, P. bockmanni, and Astyanax lacustris) using a combination of genomic, cytogenetic, and bioinformatic protocols. We also compared our data with the available satellitome of P. paranae. We described 57 satellite DNA (satDNA) families of P. fasciatus (80 variants), 50 of P. bockmanni (77 variants), and 33 of A. lacustris (54 variants). Our analyses demonstrated that several sequences were shared among the analyzed species, while some were restricted to two or three species. In total, we isolated 104 distinctive satDNA families present in the four species, of which 10 were shared among all four. Chromosome mapping revealed that the clustered satDNA was mainly located in the subtelomeric and pericentromeric areas. Although all Psalidodon species demonstrated the same pattern of clusterization of satDNA, the number of clusters per genome was variable, indicating a high dynamism of these sequences. In addition, our results expand the knowledge of the As51 satellite DNA family, revealing that P. bockmanni and P. paranae exhibited an abundant variant of 39 bp, while P. fasciatus showed a variant of 43 bp. The majority of satDNAs in the satellitomes analyzed here presented a common library repetitive sequence in Psalidodon and Astyanax, with abundance variations in each species, as expected for closely related groups. In addition, we concluded that the most abundant satDNA in Psalidodon (As51) passed through a diversification process in this group, resulting in new variants exclusive of Psalidodon.
Association of NKAPL rs1635 With Cognitive Function in Early-Onset Schizophrenia
Authors: Yang Yang , Su Yi , Wei Guiming , Kang Zhewei , Lu Zhe , Liao Yundan , Lu Tianlan , Yan Hao , Yue Weihua , Qin Ying , Zhang Yuyanan 
Published: June 2022

BACKGROUND: Schizophrenia is a severe mental disorder with high heritability, and cognitive dysfunction is one of the core features. Growing evidence suggests the genetic risk of schizophrenia may contribute to cognitive impairments. The variant rs1635 (nucleotide sequence: c.455C&gt;A; amino acid sequence: T152N) located on the (NFKB activating protein like) NKAPL gene confers risk for schizophrenia and might play a role in the neurodevelopmental process, which is particularly relevant to cognitive function. However, the relationship between rs1635 and cognitive function remains unclear.METHODS: A total of 130 patients with early-onset schizophrenia (EOS) and 300 patients with adult-onset schizophrenia (AOS) of Han Chinese were recruited and underwent neurocognitive tests by using the MATRICS Consensus Cognitive Battery (MCCB). The NKAPL rs1635 was genotyped by using DNA sequencing. The peripheral blood NKAPL mRNA expression level was examined in 152T or 152N carriers (n = 20) in EOS patients, by using the qRT-PCR. The phosphorylation level of NAKPL T152N polymorphism was detected by cell experiments. In utero electroporation of mouse embryos was examined to explore the effect of Nkapl on neuronal migration.RESULTS: Compared with rs1635 AA and AC carriers, CC (the CC genotype encodes the protein NKAPL-152T) carriers of EOS patients performed better in cognitive domain of speed of processing (t = 2.644, p = 0.009), trail making test (t = 2.221, p = 0.028) and category fluency (t = 2.578, p = 0.011). However, patients with AOS exhibited no significant differences in seven domains among the three genotype groups. There were no significant differences in cognitive performance between EOS and AOS. In EOS patients, NKAPL mRNA level in NKAPL-152N carriers is significantly lower than that of NKAPL-152T carriers. The phosphorylation level of NKAPL-152N is significantly decreased compared to NKAPL-152T. In utero electroporation showed that Nkapl deletion impairs the embryonic radial migration process.CONCLUSION: The present study found that NKAPL rs1635 was associated with cognitive impairments and peripheral blood mRNA expression level in EOS patients. The NKAPL full-length protein is required for embryonic cortical neuronal migration. The phosphorylation level of NKAPL-152N is significantly decreased. The NKAPL T152N may affect the NAKPL mRNA expression level and embryonic cortical neuronal migration by regulating the NAKPL protein phosphorylation. These data suggest that NKAPL rs1635 affects cognitive function by regulating early brain development in early-onset schizophrenia.
Milk Fat Globule Membrane Attenuates Acute Colitis and Secondary Liver Injury by Improving the Mucus Barrier and Regulating the Gut Microbiota
Authors: Wu Zhenhua , Liu Xiaoyi , Huang Shimeng , Li Tiantian , Zhang Xiangyu , Pang Jiaman , Zhao Junying , Chen Lijun , Zhang Bing , Wang Junjun , Han Dandan 
Published: June 2022

ObjectiveInflammatory bowel disease (IBD) often occurs along with extraintestinal manifestations, including hepatic injury. Milk fat globule membrane (MFGM) is an active substance with a potential anti-inflammation activity. However, its alleviated effect and mechanisms in IBD as well as the IBD-induced secondary liver injury are still unclear.MethodsC57BL/6J mice were administered with a 21-day oral gavage of MFGM, followed by 7 days of drinking water with 4% dextran sulfate sodium (DSS). Disease activity index (DAI), histological features, and cytokines of the colon and liver were evaluated. Then, RNA-seq of the colon and liver was conducted. The gut microbiota was assessed by analyzing 16S rRNA gene sequences, and finally the integrity and the function of the mucus barrier were evaluated by Alcian blue staining, real-time quantitative PCR, and ELISA.ResultsProphylactic MFGM treatment was effective against colitis to include effects in body weight loss, DAI score, colonic length, intestinal pathology, and histological score. Additionally, prophylactic MFGM decreased the levels of interleukin (IL)-1β, IL-6, and myeloperoxidase in colonic tissue, while it increased the IL-10 level. Moreover, the gene expressions of MUC2, MUC4, Reg3b, and Reg3g associated with the production of the molecular mediator of immune response, membrane invagination, and response to protozoan were strikingly upregulated when administered with MFGM. On the other hand, the beneficial effects of MFGM were related to the enriched abundance of genera such as Faccalibacumum and Roseburia in feces samples. Consistently, the administration of MFGM was also found to alleviate DSS-induced hepatic injury. Furthermore, the glutathione transferase activity pathway was enriched in the liver of MFGM-treated mice after DSS administration. Mechanistically, prophylactic MFGM enhanced the mucosal barrier by increasing the gene levels of Reg3b and Reg3g. Meanwhile, the alleviation of MFGM on liver injury was dependent on the reduced hepatic oxidative stress.ConclusionsMFGM attenuated colitis and hepatic injury by maintaining the mucosal barrier and bacterial community while inhibiting oxidative stress, which might be an effective therapy of hepatic injury secondary to IBD.