bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular and Anatomical Characterization of Parabrachial Neurons and Their Axonal Projections
Authors: Pauli Jordan L. , Chen Jane Y. , Basiri Marcus L. , Park Sekun , Carter Matthew E. , Sanz Elisendra , McKnight G. Stanley , Stuber Garret D. , Palmiter Richard D. 
Published: July 2022

AbstractThe parabrachial nucleus (PBN) is a major hub that receives sensory information from both internal and external environments. Specific populations of PBN neurons are involved in behaviors including food and water intake, pain sensation, breathing regulation, as well as learning and responding appropriately to threatening stimuli. However, it is unclear how many PBN neuron populations exist and how different behaviors may be encoded by unique signaling molecules or receptors. Here we provide a repository of data on the molecular identity, spatial location, and projection patterns on dozens of PBN neuron subclusters. Using single-cell RNA sequencing, we identified 21 subclusters of neurons in the PBN and neighboring regions. Multiplexed in situ hybridization showed many of these subclusters are localized to distinct PBN subregions. We also describe two major ascending pathways that innervate distinct brain regions by analyzing axonal projections in 21 Cre-driver lines of mice. These results are all publicly available for download and provide a foundation for further interrogation of PBN functions and connections.
Loop stacking organizes genome folding from TADs to chromosomes
Authors: Hafner Antonina , Park Minhee , Berger Scott E. , Nora Elphège P. , Boettiger Alistair N. 
Published: July 2022

AbstractWhile population level analyses reveal significant roles for CTCF and cohesin in mammalian genome organization, their contribution to chromatin structure and gene regulation at the single-cell level remain incompletely understood 1–4. Here, we use a super-resolution microscopy approach, Optical Reconstruction of Chromatin Architecture (ORCA) 5 to measure the effects of removal of CTCF or cohesin on genome folding across genomic scales. In untreated embryonic stem cells, we observe intricate, frequently stacked loops of chromatin which are largely dissolved upon cohesin removal. The loops compact chromatin at the < 3 Mb scale, increasing proximity between sequences not only within but also between TADs. We find multi-way contacts among loop anchors, preferentially at TAD borders, and these hubs largely dissolve upon CTCF degradation. CTCF-hubs bridge intervening TAD boundaries while keeping border distal regions from neighboring TADs apart outside the hub. Cohesin dependent loops at the < 3 Mb scale impede mixing at larger chromosomal scales through steric effects of loop stacking, dramatically reducing genomic cross-talk. Disruption of this ordered chromosomal structure led to increased cell-cell variability in gene expression, exceeding changes to average expression. Together our data revise the TAD-centric understanding of CTCF and cohesin, and provide a multi-scale, structural picture of how they organize the genome on the single-cell level through distinct contributions to loop stacking.
Senescence in yeast is associated with chromosome XII fragments rather than ribosomal DNA circle accumulation
Authors: Zylstra Andre , Hadj-Moussa Hanane , Horkai Dorottya , Whale Alex , Piguet Baptiste , Houseley Jonathan 
Published: July 2022

AbstractThe massive accumulation of extrachromosomal ribosomal DNA circles (ERCs) in yeast mother cells has been long cited as the primary driver of replicative ageing. ERCs arise through ribosomal DNA (rDNA) recombination and a wealth of genetic data connects rDNA instability events giving rise to ERCs with shortened lifespan and other ageing pathologies. However, we understand little about the molecular effects of ERC accumulation. Here we studied ageing in the presence and absence of ERCs, and unexpectedly found no evidence of gene expression differences that might indicate stress responses or metabolic feedback caused by ERCs. Neither did we observe any global change in the widespread disruption of gene expression that accompanies yeast ageing, altogether suggesting that ERCs are largely inert. Much of the differential gene expression that accompanies ageing in yeast was actually associated with markers of the Senescence Entry Point (SEP), showing that senescence rather than age underlies these changes. Cells passed the SEP irrespective of ERCs, but we found the SEP to be associated with copy number amplification of a region of chromosome XII between the rDNA and the telomere (ChrXIIr), which arises in aged cells due to rDNA instability but through a different mechanism to ERCs. Therefore, although rDNA copy number increases dramatically with age due to ERC accumulation, our findings implicate ChrXIIr, rather than ERCs, as the primary driver of senescence during budding yeast ageing.
A knowledge graph completion model integrating entity description and network structure
Authors: Yu Chuanming , Zhang Zhengang , An Lu , Li Gang 
Published: July 2022

PurposeIn recent years, knowledge graph completion has gained increasing research focus and shown significant improvements. However, most existing models only use the structures of knowledge graph triples when obtaining the entity and relationship representations. In contrast, the integration of the entity description and the knowledge graph network structure has been ignored. This paper aims to investigate how to leverage both the entity description and the network structure to enhance the knowledge graph completion with a high generalization ability among different datasets.Design/methodology/approachThe authors propose an entity-description augmented knowledge graph completion model (EDA-KGC), which incorporates the entity description and network structure. It consists of three modules, i.e. representation initialization, deep interaction and reasoning. The representation initialization module utilizes entity descriptions to obtain the pre-trained representation of entities. The deep interaction module acquires the features of the deep interaction between entities and relationships. The reasoning component performs matrix manipulations with the deep interaction feature vector and entity representation matrix, thus obtaining the probability distribution of target entities. The authors conduct intensive experiments on the FB15K, WN18, FB15K-237 and WN18RR data sets to validate the effect of the proposed model.FindingsThe experiments demonstrate that the proposed model outperforms the traditional structure-based knowledge graph completion model and the entity-description-enhanced knowledge graph completion model. The experiments also suggest that the model has greater feasibility in different scenarios such as sparse data, dynamic entities and limited training epochs. The study shows that the integration of entity description and network structure can significantly increase the effect of the knowledge graph completion task.Originality/valueThe research has a significant reference for completing the missing information in the knowledge graph and improving the application effect of the knowledge graph in information retrieval, question answering and other fields.
The impact of regional economic incentives on underwriters' market share in China
Authors: Gong Stephen , Shan Liwei , Yu Li 
Published: July 2022

PurposeTo examine whether and how the different levels of regional economic incentives would have an effect on underwriters' market share in general.Design/methodology/approachDrawing on Chinese IPO firms during the period 2006-2016, this study examines the impact of different levels of regional economic incentives on underwriters' market share.FindingsThe authors find that regional economic incentives have a positive impact on underwriters' market share and that local economic incentives have a significantly stronger impact than central economic incentives. Furthermore, the authors find that IPO firms with underwriters driven by regional economic incentives experience worse post-IPO performance than firms with underwriters driven by central economic incentives, which do not experience a significant decline in post-IPO performance.Originality/valueTaken together, the authors’ findings are consistent with the notion that performance assessment motivates officials at various levels of government to bring companies in their jurisdiction to the IPO market prematurely. In addition, the results indicate that central economic incentives play a significant role in driving China's macroeconomic development and market-oriented system reforms. As such, they are one of the major driving forces behind China's market-oriented system reforms.
Participant or spectator? Comprehending the sport sponsorship process from different perspectives
Authors: Koronios Konstantinos , Thrassou Alkis , Ntasis Lazaros , Sakka Georgia 
Published: July 2022

PurposeDespite the fact this is already known over how sports spectators interpret sponsorship content, less is known about participants’ sentiments toward sponsors, which are sometimes crucial to the survival of participation sporting events. Various researchers have examined at how spectators recognize or recall sponsors, but very few have explored it from the viewpoint of sport participants. This research portrays a shift in focus from spectator-based to participant-based marketing by studying the differences over how participants and spectators perceive sport sponsorship, recognizing the current knowledge gap regarding the process of sponsorship in participant-based sports. Sponsorship previous research has focused on the effectiveness of sport sponsorships utilizing variables such sponsor recognition, desire to sponsors’ products and anticipated sponsorship advantages (Koronios and Dimitropoulos, 2020). The examination of sport sponsorship from the standpoint of the sport spectator is something that many of the past studies have in common. The application of past research analysing sport sponsorship from a distinct perspective: that of the sport participant, distinguishes the present study. In addition, the present study explores the distinctions in sponsorship effectiveness between spectators and participants, a topic which hasn't been addressed at before in sponsorship literature.Design/methodology/approachThis study depicts a move away from spectator-based to participant-based marketing, utilizing contemporary implicit physiological variables to measure participants’ and spectators’ awareness of and attitude toward sponsors, and it proposes a model of their purchase intentions toward real sponsors of a martial event. A total of 1,332 questionnaires were collected and analysed.FindingsFactors such as sport involvement, social media use, sincerity and beliefs of sponsorship were found to have a significant effect on purchase intention of spectators and participants. And crucial differences in the significance of various antecedent factors between spectators and participants were detected in the formation of positive purchase and word-of-mouth intentions.Originality/valueThis paper gives prominence to contemporary methodological advances in the field of implicit social cognition and their relevance to sponsorship. This is the first sponsorship research which examines the effectiveness of sponsorship variables under the implicit–explicit variables dichotomy.
Post-pandemic assessment of online teaching and learning in higher business education
Authors: Chen Cindy , Landa Sabrina , Padilla Aivanna , Yur-Austin Jasmine 
Published: July 2022

PurposeAcademic institutions with sufficient resources rapidly deployed virtual teaching technologies and training to minimize disruption following the Spring 2020 COVID-19 pandemic. This paper shares a College of Business experience to provide insights for administrators tasked with future online course scheduling decisions.Design/methodology/approachAn online questionnaire was administered in Fall (2020) to measure student perceptions of online learning using Likert-style questioning. The researchers used the results to build a structural equation model to differentiate perceptions between online course modalities and curriculum rigor (graduate/undergraduate, upper/lower divisions) and field of study (quantitative/qualitative, MBA/MS) factors.FindingsThe empirical findings support the notation that graduate and undergraduate learners exhibit different preferences of online modalities. The findings further demonstrate that curriculum rigor factors and field of study influence student satisfaction of online courses. The evidence also suggests varying dependence on instructor competency and technology effectiveness across asynchronous, hybrid, and synchronous modalities.Practical implicationsWhile this study is limited to the results of one higher education institution during a tumultuous period, as online education trends increase, the authors' methodology can be adapted and scaled to support post-pandemic administrative decision-making.Originality/valueThe research provides a new dimension on the perspectives of online learners through gathering perceptions in a timely student-centered survey administered during the emergency alternative modes of instruction. The research explores certain predictive factors to better align online modalities with learner satisfaction.
Emergency responders’ moral sensemaking: the influence of compartmentalization
Authors: Kalkman Jori Pascal , Kramer Eric-Hans 
Published: July 2022

PurposeEmergency organizations allocate specific tasks to responders in an attempt to resolve increasingly complex incidents. Many studies take a pragmatic perspective by studying how emergency organizations can more effectively compartmentalize response tasks. Yet, the effects of compartmentalization on responders' sensemaking of moral issues (i.e. moral sensemaking) has received almost no attention.Design/methodology/approachBased on existing research, the authors bring together different insights on the relation between compartmentalization and emergency responders’ sensemaking of moral issues.FindingsThe authors demonstrate that emergency organizations may undermine the moral sensemaking of responders through introducing moral blind spots and moral dissociation or, instead, enable moral sensemaking through enhancing moral agency and awareness. The authors argue that emergency organizations need to induce moral sense-discrediting among responders to enhance their moral sensemaking. Finally, the authors conclude with discussing two types of compartmentalizing tasks, functional concentration and the holographic metaphor, to show that the latter is most likely to enhance moral sensemaking among emergency responders.Originality/valueThis study introduces moral sensemaking to the emergency management literature and investigates how organizational design influences it.
Value stream mapping from the customer's perspective: expanding concepts, representations and key performance indicators based on a typical real case study
Authors: Guimarães Leonardo de Aragão , Jardim Eduardo Galvão Moura , Guimarães Marujo Lino 
Published: July 2022

Purpose
This study aims to improve the buying experience for both customers and providers by presenting a conceptual basis which seeks to expand the usual understanding, representation, mapping and measurements of the different value and non-value stages of a customer purchase journey (CPJ).

Design/methodology/approach
Inspired by the precepts of lean thinking, with emphasis on the value stream mapping method, the approach is based on an in-depth analysis of a real and typical e-commerce acquisition of an electronic customised product (a mobile phone) during the COVID-19 pandemic.

Findings
This study demonstrates different types of consumer stages, values and wastes for the CPJ. This allowed the development of a mathematical formulation – named customer journey engineering (CJE) – from which improvements of the different categories can be identified. Exemplifying with those whose implementations require no further efforts or costs, the following results could be readily obtained in the case studied: a reduction of 96 h of non-value activities, an improvement of approximately 15% of the established index for customer satisfaction and avoidance of loss worth US$50 for the analysed customer.

Research limitations/implications
The consistency and applicability of the qualitative and quantitative findings presented here should be examined further in other customer purchase scenarios, allowing enhancements of the CJE approach.

Originality/value
Regardless of the context in question, this investigation attempts to identify and precisely define any common universal elements, often overlooked, which constitute the structure of any CPJ and are crucial for its understanding and improvement.
Operational excellence through triple bottom line in emerging countries: a systematic review and conceptual model proposal in production research
Authors: Henriquez Rafael , Muñoz-Villamizar Andrés , Santos Javier 
Published: July 2022

Purpose
This research has the following specific objectives: provide an overview of the literature addressing operational excellence (OE) and OE in emerging countries; propose an integrative framework of OE attributes; and propose future research directions for the OE literature.

Design/methodology/approach
To remain competitive in industry, many organizations are implementing OE initiatives. However, achieving OE in emerging markets has been a pressing challenge due to the scarce knowledge on how it works. Through a systematic review of the literature, this study aims to contribute to a deeper understanding of the concept of OE in emerging countries through the triple bottom line.

Findings
This study has found nine attributes of OE based on the three pillars of sustainability or triple bottom line (economic, environmental and social). Four of them were the most cited and served to build the proposed framework. The analysis concludes that most of the studies related to OE in emerging countries have been carried out in Asia. Finally, based on the results obtained from the literature review, six points of future research are proposed based on three research directions: OE in emerging countries, active participation by employees and culture generation and finally, research methods.

Originality/value
The gap that is filled in this study is that some authors have studied the application of OE in emerging countries, specifically on the Asian continent. In those cases, the literature is either oriented to describing OE from different perspectives or to its application in different companies, some of which are emerging countries. However, to the best of the authors’ knowledge, there is no study that describes the specific and complete aspects that make up a model of OE and which can show how this model can be applied in emerging countries.
Intensive distribution and sales promotion for improving customer-based brand equity (CBBE), re-purchase intention and word-of-mouth (WOM)
Authors: Langga Ande , Kusumawati Andriani , Alhabsji Taher 
Published: July 2022

PurposeInvestigating the influence of intensive distribution and sales promotion towards customer-based brand equity, repurchase intention and word-of-mouth (WOM) (study on Suzuki car owners in PT Surya Batara Mahkota Wilayah Nusa Tenggara Timur).Design/methodology/approachThe research was conducted in East Nusa Tenggara (NTT) and the analysis unit was customers of PT. Surya Batara Mahkota NTT (PT SBM NTT) as the owner of the Suzuki car. The population is 1,782 Suzuki car owners who bought their cars from PT SBM NTT, based on data from 2015. The sampling technique is the multi-stage area sampling.FindingsIncentives distribution had significant and positive influence towards brand equity and repurchase intention. Sales promotion had significant and positive influence towards word-of-mouth (WOM), but it did not have influence towards brand equity. Brand equity had significant influence towards repurchase intention and WOM. On the other hand, repurchase intention did not have influence towards WOM.Originality/valueThe originality of this study was that the researchers did not find a previous study that discussed the relationship between intensive distribution and repurchase intention, between sales promotion and WOM and between customer-based brand equity and WOM. Previous studies used different variables as determinants of positive WOM.
Generational cohorts and their predisposition toward entrepreneurship in an emerging economy
Authors: Arkorful Helen , Hilton Sam Kris , Awaah Fred 
Published: July 2022

PurposeThe study investigates the predisposition of generational cohorts toward entrepreneurship in an emerging economy as entrepreneurship has arguably become a panacea for unemployment and sustainable economic development.Design/methodology/approachThe study adopts descriptive and cross-sectional survey designs. The study also employs quantitative approach to collect the data from 1,000 workers in 20 selected private and public organizations in Ghana. The data were analyzed using descriptive statistics, correlation and hierarchical regression techniques.FindingsThe results reveal that baby boomers and Generation Z (Gen Z) have a higher predisposition toward entrepreneurship, while Generation X (Gen X) and Generation Y (Gen Y) have a lower predisposition toward entrepreneurship. However, the study found that baby boomers are more predisposed to entrepreneurship than all the generational cohorts. Furthermore, a generation may become entrepreneurs regardless of their gender. Finally, individuals with higher educational qualification (i.e. masters and doctorate) are more likely to become entrepreneurs in a given generation.Practical implicationsThe findings imply that entrepreneurial opportunities (such as ease of doing business, favorable business regulations, access to credit facilities, low interest rate, ease of registering business, start-up capital, etc.) should be created by government and its stakeholders to serve as stimuli for members of these generations (particularly baby boomers and Gen Z) to participate fully in entrepreneurial activities. In addition, the culture of “go to college and graduate with the expectation of government employment” and “job for life” should be discouraged to allow members of Gen X and Gen Y build up entrepreneurial mindsets.Originality/valueThis study contributes to generational cohorts and entrepreneurship literature by providing a perspective from the cultural and socio-economic background of an emerging economy. Additionally, this study demonstrates that irrespective of gender, one may become an entrepreneur and highly educated individuals tend be entrepreneurs.