bluuebunny/crossref_metadata_2025_split huggingface dataset:
ISSLS prize in clinical science 2022: accelerated disc degeneration after pubertal growth spurt differentiates adults with low back pain from their asymptomatic peers
Authors: Aavikko Anni , Lohman Martina , Ristolainen Leena , Kautiainen Hannu , Österman Kalevi , Schlenzka Dietrich , Lund Teija 
Published: March 2022

Abstract
Purpose
In this prospective observational cohort study, the development of lumbar intervertebral discs (LIVD) on magnetic resonance imaging (MRI) was investigated from childhood to adulthood with emphasis on the possible association of disc degeneration (DD) to low back pain (LBP).

Methods
In 2021, 89 subjects who were enrolled in 1994 in a longitudinal study with lumbar spine MRI at ages 8, 11 and 18 were invited to participate in a long-term follow-up comprising a clinical examination, selected patient-reported outcome measures and a lumbar spine MRI. We assessed all MRIs (three lowest LIVDs) with the Pfirrmann summary score, and the ratio of signal intensity of nucleus pulposus to signal intensity of cerebrospinal fluid (SINDL). We further analyzed whether disc changes at any age were associated with self-reported LBP at age 34.

Results
Of the 48 subjects in the follow-up, 35 reported LBP at age 34. The Pfirrmann summary score significantly increased with age (p < 0.001). Subjects reporting LBP at age 34 demonstrated statistically significantly higher summary scores at age 18 and 34 compared to asymptomatic subjects (p = 0.004 at age 18, and p = 0.039 at age 34). SINDL significantly decreased with age (p < 0.001 for all levels separately), but no significant differences between subjects with or without LBP at age 34 were noticed.

Conclusion
Subjects with LBP at age 34 had more widespread or severe DD already at age 18 compared to those without LBP.
Influence of perioperative SARS-CoV-2 infection on mortality in orthopaedic inpatients with surgically treated traumatic fractures
Authors: Granqvist Mathias , Hedberg Pontus , Nauclér Pontus , Enocson Anders 
Published: March 2022

Abstract
Background
SARS-CoV-2 has had an extensive influence on orthopaedic surgery practice and has been associated with an increased risk of mortality. There is limited evidence of how this pertains to acute orthopaedic surgery with inpatient care.

Methods
A retrospective cohort study on traumatic fracture patients requiring inpatient care between February 25, 2020 and March 25, 2021 was conducted. Patients were grouped by perioperative SARS-CoV-2 infection, defined as a positive SARS-CoV-2 test from 7 days before to 7 days after orthopaedic surgery, and compared using linear regression and Cox proportional hazards model for primary outcome 30-day mortality and secondary outcome hospital length of stay.

Results
In total, 5174 adults with a length of stay ≥ 48 h and an orthopaedic procedure due to a registered traumatic fracture were admitted from February 25, 2020 and discharged before March 26, 2021. Among the 5174 patients, 65% (3340/5174) were female, 22% (1146/5174) were 60–74 years and 56% (2897/5174) were 75 years or older. In total, 144 (3%) had a perioperative SARS-CoV-2 infection. Perioperative SARS-CoV-2 infection was associated with an increased 30-day mortality (aOR 4.19 [95% CI 2.67–6.43], p < 0.001). The median (IQR) length of stay after surgery was 13 days (IQR 6–21) for patients with, and 7 days (IQR 2–13) for patients without, perioperative SARS-CoV-2 infection.

Conclusions
Perioperative SARS-CoV-2 infection increased 30-day mortality risk and hospital length of stay for traumatic fracture patients requiring inpatient surgical care. Pre- and postoperative infection were both associated with similar increases in mortality risk.
5-aminolevulinic acid and sodium fluorescein in IV ventricle ependymoma surgery: preliminary experience comparing the two techniques
Authors: Boschi Andrea , Lastrucci Giancarlo , Pisano Antonio , Becattini Eleonora , Buccoliero Annamaria , Della Puppa Alessandro 
Published: March 2022

Abstract
Purpose
The aim of this study is to compare the use of 5-aminolevulinic acid (5-ALA) and sodium fluorescein (SF) in IV ventricular ependymoma (IVEP) surgical resection.

Methods
In this retrospective study, six patients with IVEP were enrolled. Gender ratio 2:1 male to female, with mean age 38.9 years old. A 5-ALA oral dose of 20 mg/kg and a SF intravenous dose of 2 mg/kg were administered. Telo-velar approach, operative microscope, and intraoperative monitoring were used in all the operations. We retrospectively compared the two fluorescence techniques at four steps during the surgical procedure: step 1: exposure of the tumor; step 2: dissection of the lesion from the cerebellum; step 3: assessment of the tumor borders and differentiation from normal tissue at the base of implants; and step 4: evaluation of possible residual tissue in the surgical cavity.

Results
At the first step, the ependymomas resulted well delineated by both fluorescent agents. In this step, 5-ALA was particularly helpful in the case of recurrent ependymoma. At step 2, 5-ALA provided a better identification of the ependymoma boundaries and distinction from cerebellum hemispheres than SF. In steps 3 and 4, SF was really helpful to detect tumor tissue.

Conclusion
According to our experience, fluorescence-guided surgery of IVEP with 5-ALA and SF is useful to maximize surgical resection with less risk of brainstem injury. Both fluorescence techniques are helpful in different steps of IVEP resection. However, further studies are needed to confirm our preliminary data.
A discrete-to-continuum model of protein complexes
Authors: Mariano Paolo Maria , Bacci Marco 
Published: March 2022

AbstractOn the basis of a tensor representation of protein shape, obtained by an affine decomposition of residue velocity, we show how to identify actions at continuum scale for both single proteins and their complexes in terms of power equivalence. The approach constructs and justifies a continuum modeling of protein complexes, which avoids a direct, atomistic-based, simulation of the whole complex, rather it focuses (in a statistical sense) on a single protein and its interactions with the neighbors. In the resulting setting we also prove the existence of equilibrium configurations (native states) under large strains.
Chemical characterisation is rough: the impact of topography and measurement parameters on energy-dispersive X-ray spectroscopy in biominerals
Authors: Shirley Bryan , Jarochowska Emilia 
Published: March 2022

AbstractEnergy dispersive X-ray microscopy (EDX) is a widely available, inexpensive method of characterizing the in-situ elemental composition of samples in Earth and life sciences. Common protocols and textbooks focussing on material sciences address EDX analysis of metallic samples that can be polished perfectly, whereas geoscientists often investigate specimens with prominent topography and composed of light, difficult to resolve elements. This is further compounded by the scarcity of literature surrounding the methodology of SEM–EDX in the field of palaeontology, leading to common misinterpretations and artefacts during data acquisition. Here, the common errors in elemental composition obtained with EDX arising from surface topography and from parameters subject to user decisions are quantified. As a model, fossil bioapatite (conodonts) and abiotic Durango apatite are used. It is shown that even microscale topography can distort measured composition by up to 34%, whereas topographic features such as tilt with respect to the electron beam lead to differences of up to 85%. Working distance was not the most important parameter affecting the results and led to differences in composition of up to 13%, whereas the choice of standard and its levelling with the sample surface led to inaccuracy reaching 33%. EDX results can be also affected by beam damage and the effects of acceleration voltage on sample acquisition and resolution are quantified. An estimate is provided of the severity of errors associated with samples which cannot satisfy preparation requirements for EDX fully, such as holotypes, and with user decisions. Using a palaeontological example, recommendations are offered for the best parameters and the relative importance of error sources are assessed.
Predicting spatially heterogeneous invasive spread: Pyracantha angustifolia invading a dry Andean valley in northern Argentina
Authors: Plenderleith Fiona A. , Irrazabal Valentina A. , Burslem David F. R. P. , Travis Justin M. J. , Powell Priscila Ana 
Published: March 2022

AbstractUnderstanding the drivers of invasive species spread is key to designing optimal management programmes for controlling them. Population models, parameterized from demographic and dispersal data, are useful for simulating invasion when long-term observations are lacking, which is particularly important when invasions are occurring rapidly. We aimed to understand how the spread ofPyracantha angustifolia(an invasive ornamental shrub) is influenced by heterogeneity in local conditions within a dry inter-montane valley in northwestern Argentina that is currently experiencing rapid urban expansion and concomitant reduction in cattle farming. Field surveys demonstrated that individuals growing in shrublands have a higher fecundity than those in grassland and rocky habitats. Furthermore, grazing pressure of cattle and horses substantially reduces fecundity and impacts the relationship between age and fecundity. We incorporated our field-estimated habitat and age-specific fecundities into both analytical integro-difference equation models and individual-based models to predict rates of spread across each habitat type in the presence or absence of grazing pressure. Results indicate that the rate of spread would be substantially higher in shrublands (by up to 33%) and highlight that grazing pressure can, through direct consumption of seeds by livestock, substantially depress the rate of spread (by up to 53%). These results suggest that shrubland areas in the valley are most vulnerable to invasion and that grazing may help to reduce the impact ofPyracantha.This suggests that a synergism between urban expansion and reduction in domestic livestock density contributes to the spread of an invasive species in this environment.
Predicting current and future distribution of Hovenia dulcis Thunb. (Rhamnaceae) worldwide
Authors: Bergamin Rodrigo Scarton , Gama Mafalda , Almerão Maurício , Hofmann Gabriel Selbach , Anastácio Pedro Manuel 
Published: March 2022

AbstractBiological invasions are increasingly recognized as one of the major threats to biodiversity. The Japanese raisin tree (Hovenia dulcis) is native to East Asia, however, in southeastern South America this species has become one of the most pervasive invaders. Hovenia dulcis has many biological characteristics that favor the process of invasion and few studies have indicated changes in the structure and composition of native plant communities where this species has become invader. Given the invasiveness shown in southeastern South America, our main goal was to identify the potentially suitable habitats for this invasive species at a global scale. In this sense, we modeled the potential distribution of H. dulcis along the terrestrial areas worldwide using an ensemble forecasting approach. Additionally, the percentage of overlapping biodiversity hotspot areas with the currently suitable areas for this species was calculated. Our results revealed that the current potential H. dulcis range is equivalent to 7.88% (12,719,365 km2) of the terrestrial area worldwide. For the future scenarios of climate change, the potential distribution area tends to have a small reduction. However, significant suitable areas were identified for H. dulcis range in the northern limits of the boreal distribution. Currently, around 17% of biodiversity hotspot areas overlap with the suitable areas for H. dulcis occurrence. In summary, given that the prevention is well-recognized as a more effective management action against invasive alien species, it is essential to implement policies to prevent H. dulcis introduction in suitable areas worldwide, as well as local population control, especially in biodiversity hotspots.
The alien invasive forest pathogen Heterobasidion irregulare is replacing the native Heterobasidion annosum
Authors: Garbelotto M. , Lione G. , Martiniuc A. V. , Gonthier P. 
Published: March 2022

AbstractInvasions by alien pathogens are a major threat to forest conservation. The North American fungal pathogen of conifers Heterobasidion irregulare, inadvertently introduced in Central Italy in the 1940s, has been spreading causing high mortality of Italian stone pine (Pinus pinea). While invading newfound niches, H. irregulare has established itself in the current range of the native congener H. annosum. The aims of this study were to determine whether in time: (I) H. irregulare populations may be increasing in size; (II) H. irregulare may be replacing H. annosum, rather than simply coexisting with it; and, (III) H. annosum may disappear in forests infested by H. irregulare. The presence, abundance and distribution of H. annosum and H. irregulare were assessed through an aerobiological assay replicated ten years apart in a forest in which both species have been coexisting. Replacement index (RI), Markov chains and geometric progressions were used to model the interspecific interaction between the two species and to assess the invasiveness of H. irregulare. Results showed that, in 10 years, the incidence of H. annosum dropped from 39.4 to 6.1%, while that of H. irregulare increased from 57.6 to 81.8%, with the alien pathogen replacing the native species (RI = 84.6%) and spreading at a maximum rate of 139 ha/year. Although our models show that the extinction of H. annosum may be unlikely, the ability of H. irregulare to replace it suggests the alien pathogen may also readily colonize those parts of Europe where H. annosum is more abundant than in Central Italy.
Ureaplasma-Driven Neonatal Neuroinflammation: Novel Insights from an Ovine Model
Authors: Silwedel Christine , Hütten Matthias C. , Speer Christian P. , Härtel Christoph , Haarmann Axel , Henrich Birgit , Tijssen Maud P. M. , Alnakhli Abdullah Ahmed , Spiller Owen B. , Schlegel Nicolas , Seidenspinner Silvia , Kramer Boris W. , Glaser Kirsten 
Published: March 2022

AbstractUreaplasma species (spp.) are considered commensals of the adult genitourinary tract, but have been associated with chorioamnionitis, preterm birth, and invasive infections in neonates, including meningitis. Data on mechanisms involved in Ureaplasma-driven neuroinflammation are scarce. The present study addressed brain inflammatory responses in preterm lambs exposed to Ureaplasma parvum (UP) in utero. 7 days after intra-amniotic injection of UP (n = 10) or saline (n = 11), lambs were surgically delivered at gestational day 128–129. Expression of inflammatory markers was assessed in different brain regions using qRT-PCR and in cerebrospinal fluid (CSF) by multiplex immunoassay. CSF was analyzed for UP presence using ureB-based real-time PCR, and MRI scans documented cerebral white matter area and cortical folding. Cerebral tissue levels of atypical chemokine receptor (ACKR) 3, caspases 1-like, 2, 7, and C–X–C chemokine receptor (CXCR) 4 mRNA, as well as CSF interleukin-8 protein concentrations were significantly increased in UP-exposed lambs. UP presence in CSF was confirmed in one animal. Cortical folding and white matter area did not differ among groups. The present study confirms a role of caspases and the transmembrane receptors ACKR3 and CXCR4 in Ureaplasma-driven neuroinflammation. Enhanced caspase 1-like, 2, and 7 expression may reflect cell death. Increased ACKR3 and CXCR4 expression has been associated with inflammatory central nervous system (CNS) diseases and impaired blood–brain barrier function. According to these data and previous in vitro findings from our group, we speculate that Ureaplasma-induced caspase and receptor responses affect CNS barrier properties and thus facilitate neuroinflammation.
Terahertz Measurements on Subwavelength-Size Samples Down to the Tunneling Limit
Authors: Maluski D. , Cámara Mayorga I. , Hemberger J. , Grüninger M. 
Published: March 2022

AbstractFor terahertz spectroscopy on single crystals, the wavelength $$\lambda$$
λ
often is comparable to the size of the studied samples, emphasizing diffraction effects. Using a continuous-wave terahertz spectrometer in transmission geometry, we address the effect of the sample size on the achievable accuracy of the optical properties, focusing in particular on the phase data. We employ $$\alpha$$
α
-lactose monohydrate as a paradigmatic example and compare data that were measured using apertures with diameters D in the range from 10 to 0.2 mm. For small D, strong diffraction typically invalidates a quantitative analysis of the transmitted amplitude at low frequencies. The phase data, however, can be evaluated to lower frequency and show a more systematic dependence on D. For a quantitative analysis, we employ a waveguide picture for the description of small apertures with a cylindrical bore. For D as small as 0.2 mm, corresponding to 1/D = 50 cm$$^{-1}$$

-
1

, a circular waveguide does not support propagating waves below its cut-off frequency $$1/\lambda _c$$

1
/

λ
c

= $$\omega _c/2\pi c \approx 29$$

ω
c

/
2
π
c
≈
29

cm$$^{-1}$$

-
1

. Experimentally, we confirm this cut-off for cylindrical apertures with a thickness $$d_{\text {ap}}$$

d
ap

= 1 mm. Close to $$\omega _c$$

ω
c

, the measured phase velocity is an order of magnitude larger than c, the speed of light in vacuum. The cut-off is washed out if a sample is mounted on a thin aperture with a conical bore. In this case, the phase data of $$\alpha$$
α
-lactose monohydrate for D = 0.2 mm can quantitatively be described down to about 10 cm$$^{-1}$$

-
1

if the waveguide-like properties of the aperture are taken into account in the analysis.
Elastic 3D–2D Image Registration
Authors: Striewski Paul , Wirth Benedikt 
Published: March 2022

AbstractWe propose a method to nonrigidly align a three-dimensional (3D) volumetric image with a two-dimensional (2D) planar image representing a projection of the deformed volume. The application in mind comes from biological studies in which 2D intravital microscopy videos of living tissue are recorded, after which the tissue is excised and a more detailed 3D volume microscopy is performed. Coregistration of both data sets allows to combine the temporal (but 2D) information with more detailed spatial 3D information. Our approach is variational and uses a hyperelastic deformation regularization, as is appropriate for biological material. As a particular feature, the out-of-plane deformation is estimated based on the out-of-focus blur inside the 2D microscopy image. The approach becomes computationally feasible through the use of a coarse-to-fine optimization strategy and higher-order optimization methods.
Assessment of neonatal respiratory rate variability
Authors: Coleman Jesse , Ginsburg Amy Sarah , Macharia William M. , Ochieng Roseline , Chomba Dorothy , Zhou Guohai , Dunsmuir Dustin , Karlen Walter , Ansermino J. Mark 
Published: March 2022

AbstractAccurate measurement of respiratory rate (RR) in neonates is challenging due to high neonatal RR variability (RRV). There is growing evidence that RRV measurement could inform and guide neonatal care. We sought to quantify neonatal RRV during a clinical study in which we compared multiparameter continuous physiological monitoring (MCPM) devices. Measurements of capnography-recorded exhaled carbon dioxide across 60-s epochs were collected from neonates admitted to the neonatal unit at Aga Khan University-Nairobi hospital. Breaths were manually counted from capnograms and using an automated signal detection algorithm which also calculated mean and median RR for each epoch. Outcome measures were between- and within-neonate RRV, between- and within-epoch RRV, and 95% limits of agreement, bias, and root-mean-square deviation. Twenty-seven neonates were included, with 130 epochs analysed. Mean manual breath count (MBC) was 48 breaths per minute. Median RRV ranged from 11.5% (interquartile range (IQR) 6.8–18.9%) to 28.1% (IQR 23.5–36.7%). Bias and limits of agreement for MBC vs algorithm-derived breath count, MBC vs algorithm-derived median breath rate, MBC vs algorithm-derived mean breath rate were − 0.5 (− 2.7, 1.66), − 3.16 (− 12.12, 5.8), and − 3.99 (− 11.3, 3.32), respectively. The marked RRV highlights the challenge of performing accurate RR measurements in neonates. More research is required to optimize the use of RRV to improve care. When evaluating MCPM devices, accuracy thresholds should be less stringent in newborns due to increased RRV. Lastly, median RR, which discounts the impact of extreme outliers, may be more reflective of the underlying physiological control of breathing.
Associations Between Social Media Engagement and Vaccine Hesitancy
Authors: Al-Uqdah Lola , Franklin F. Abron , Chiu Chu-Chuan , Boyd Brianna N. 
Published: March 2022

AbstractThere has been a surge in vaccine hesitancy following the Coronavirus pandemic. This study measured the prevalence of and identified factors associated with vaccine hesitancy and social media use. An online survey was administered (n = 1050) between May and July 2021. Chi-square tests were used to examine bivariate associations with vaccine hesitancy (partially vaccinated and unvaccinated participants). Logistic regression was used to identify associations between social media use and vaccine hesitancy. Chi-square tests showed women (69.7% vs 28.2% men, padjusted = .002), African American participants (52.3% vs 17.8% white, padjusted < .001), high school diploma (54.4% vs 38.6% college degree, padjusted < .001), political unaffiliated (15.8% vs 14.5% republican, padjusted < .001), Muslim (10.0% vs 0% Jewish, padjusted < .001), and never married/single (53.9% vs 17.0% married, padjusted < .001) were more likely to be vaccine hesitant. Controlling for all demographic variables (age, race, gender, and education), more frequent use of social media for reading news was associated with lower vaccine hesitancy (OR 0.35, 99% CI 0.20, 0.63, p < 0.001). However, using social media as a source of vaccine information without any other trusted source (health department, doctor, CDC,) was associated with higher odds of being vaccine hesitant (OR 2.00, 99% CI 1.15, 3.46, p = 0.001). People who use social media without referencing trusted sources may be particularly vulnerable to disinformation or vaccine hesitant persons are more likely exposed to non-trusted social media sites as their only information source.
Stability monitoring of selected spike isotopic reference materials for isotope dilution mass spectrometry
Authors: Jakopič Rožle , Venchiarutti Célia , Hennessy Carmel , Kehoe Frances , Aregbe Yetunde 
Published: March 2022

AbstractThe provision of high quality spike isotopic reference materials is one of the objectives of the Joint Research Centre of the European Commission. They play an important part in measurements of nuclear materials for Nuclear Safeguards. Spike isotopic reference materials are prepared and certified according to the international standards. The assigned values for the isotope amount content and isotope ratios need to be verified at regular intervals. This is carried out by stability monitoring measurements. This paper will discuss the results of the stability assessment of some 242Pu and 243Am spike isotopic reference materials.