bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multicentric evaluation of the variability of digital morphology performances also respect to the reference methods by optical microscopy
Authors: Da Rin Giorgio , Seghezzi Michela , Padoan Andrea , Pajola Rachele , Bengiamo Anna , Di Fabio Anna Maria , Dima Francesco , Fanelli Alessandra , Francione Sara , Germagnoli Luca , Lorubbio Maria , Marzoni Alessandro , Pipitone Silvia , Rolla Roberta , Bagorria Vaca Maria del Carmen , Bartolini Andrea , Bonato Lisa , Sciacovelli Laura , Buoro Sabrina ,   Italian Working Group on Diagnostic Hematology of the Italian Society of Clinical Chemistry and Clinical Molecular Biology (WGDH‐SIBioC)
Published: August 2022

AbstractIntroductionDespite the important diagnostic role of peripheral blood morphology, cell classification is subjective. Automated image‐processing systems (AIS) provide more accurate and objective morphological evaluation. The aims of this multicenter study were the evaluation of the intra and inter‐laboratory variation between different AIS in cell pre‐classification and after reclassification, compared with manual optical microscopy, the reference method.MethodsSix peripheral blood samples were included in this study, for each sample, 70 May‐Grunwald and Giemsa stained PB smears were prepared from each specimen and 10 slides were delivered to the seven laboratories involved. Smears were processed by both optical microscopy (OM) and AIS. In addition, the assessment times of both methods were recorded.ResultsWithin‐laboratory Reproducibility ranged between 4.76% and 153.78%; between‐laboratory Precision ranged between 2.10% and 82.2%, while Total Imprecision ranged between 5.21% and 20.60%. The relative Bland Altman bias ranged between −0.01% and 20.60%. The mean of assessment times were 326 ± 110 s and 191 ± 68 s for AIS post reclassification and OM, respectively.ConclusionsAIS can be helpful when the number of cell counted are low and can give advantages in terms of efficiency, objectivity and time saving in the morphological analysis of blood cells. They can also help in the interpretation of some morphological features and can serve as learning and investigation tools.
The inactivation efficacy of plasma-activated acetic acid against Salmonella Typhimurium cells and biofilm
Authors: Kang Taemin , Yim Donggyun , Baek Ki Ho , Lee Yee Eun , Kim Hyun-Jun , Jo Cheorun 
Published: August 2022

Abstract

Aim
This study aimed to examine the inactivation efficacy of plasma-activated acetic acid (PAAA) against Salmonella Typhimurium cells and biofilm and elucidate underlying chemical inactivation pathway.

Methods and Results
PAAA was prepared by discharging plasma to 20 ml of 0.2% (v/v) acetic acid (AA) for 20 min (2.2 kHz and 8.4 kVpp). The count of cells and biofilms decreased by 5.71 log CFU ml−1 and 4 log CFU/cm2 after 10 min of treatment with 0.2% PAAA and 0.4% PAAA compared with control group (without any treatment), respectively. In 0.2% PAAA, the concentrations of hydrogen peroxide (H2O2) and nitrate anions were directly proportional to the plasma discharge time, whilst nitrite anion (NO2−) was not detected. However, the pH values of both 0.2% PAAA and plasma-activated water were inversely proportional to the plasma discharge time. Treatment with catalase, L-histidine, D-mannitol and sodium azide inhibited the antibacterial activity of PAAA.

Conclusion
H2O2, singlet oxygen, hydroxyl radical and NO2− are involved in the generation and decomposition of peroxynitrous acid generated from PAAA functioned as intermediate agent, which could diffuse through cell membranes of bacteria and induce cell injury.

Significance and Impact of Study
This study provides the understanding of efficacy and selectivity of PAAA which could be a novel decontamination agent.
Minding the matrix: The importance of inoculum suspensions on finger transfer efficiency of virus
Authors: Abney Sarah E. , Wilson Amanda M. , Ijaz M. Khalid , McKinney Julie , Reynolds Kelly A. , Gerba Charles P. 
Published: August 2022

Abstract

Aims
The aim of this study was to determine how the transfer efficiency of MS-2 coliphage from the toilet seat to hands and fingertip to lip differs according to the suspension of the inoculum.

Methods and Results
Hands were sampled after lifting a toilet seat which was inoculated with MS-2 on the underneath side. MS-2 was suspended in a spectrum of proteinaceous and non-proteinaceous solutions. Transfer efficiencies were greatest with the ASTM tripartite soil load (3.02% ± 4.03) and lowest with phosphate-buffered saline (PBS) (1.10% ± 0.81) for hand-to-toilet seat contacts. Finger-to-lip transfer rates were significantly different (p &lt; 0.05) depending on suspension matrix, with PBS yielding the highest transfer (52.53% ± 4.48%) and tryptose soy broth (TSB) the lowest (23.15% ± 24.27%). Quantitative microbial risk assessment was used to estimate the probability of infection from adenovirus and norovirus from finger contact with a toilet seat.

Conclusions
The greatest transfer as well as the largest variation of transfer were measured for finger-to-lip contacts as opposed to toilet seat-to-finger contacts. These factors influence the estimation of the probability of infection from micro-activity, that is, toilet seat adjustment.

Significance and Impact
Viruses may be transferred from various human excreta with differing transfer efficiencies, depending on the protein content.
Value creation and the circular economy: A tale of three externalities
Authors: Figge Frank , Thorpe Andrea Stevenson , Manzhynski Siarhei 
Published: August 2022

AbstractBy using resources more efficiently, resource users help to overcome the inherent resource scarcity on “spaceship earth.” One strategy in this context is to close resource loops and to use resources circularly. With fewer resources wasted, a more circular use of resources should also increase the efficiency of resource use and create more value. However, when resource users aim for a greater degree of efficiency, inadvertently they might contribute to resources being used less rather than more circularly and, consequently, less instead of more efficiently. We show how to assess the value that is created by the efficient use of resources for the case of linear and circular resource use. This allows us to identify three distinct types of positive externalities related to the circular use of resources: (1) systemic static externalities; (2) idiosyncratic dynamic externalities; and (3) systemic dynamic externalities. We describe how the value created by these externalities can be assessed and argue that they need to be considered when evaluating environmental resource use.
Microstructural evolution during short heat treatment of constrained groove pressed Cu‐5%Zn alloy
Authors: Yadav Prabhat Chand , Sarvesha R. , Guruprasad T. S. 
Published: August 2022

AbstractSevere plastic deformation (SPD) is a widely used technique to obtain superior material properties specially mechanical properties. Constrained groove pressing (CGP) is found to be the most attractive SPD technique for the deformation of sheets and plates. However, this technique results in microstructural inhomogeneity during processing. The microstructural inhomogeneity can be alleviated by employing a thermal cycle, which assists in controlled recovery and recrystallisation. The current work focuses on, the microstructure evolution of one pass as‐deform CGP sample followed by a short heat‐treated (SHT). A correlative imaging technique of transmission Kikuchi diffraction (TKD) and transmission electron microscopy (TEM) showed the presence of dislocation cell structure in an as‐deformed condition. The short heat treatment resulted in the transformation of the dislocation cell wall to high‐angle boundaries, with a further increase in heat‐treatment time resulting in grain growth.
Lipid profile, apolipoproteins A‐1 and B in owl monkeys (Aotus infulatus) in captivity
Authors: da Silva Wellington Bandeira , Teixeira André Luís de Souza , Oliveira Karol Guimarães , Gonzaga Camila Nunes , de Souza Aline Moreira , Almosny Nádia Regina Pereira 
Published: August 2022

AbstractBackgroundOwl monkeys (Aotus infulatus) are frequently affected by heart diseases and, as in humans, dyslipidemia is one of the predisposing factors for adverse cardiovascular events. In view of this, the study of the lipid profile and plasma apolipoproteins can contribute to the clinical management of this neotropical primate species.MethodsLipid profile as well as A‐1 and B apolipoprotein values were analyzed in 60 owl monkeys, studying their relationship with body biometry and the presence of cardiac alterations.ResultsAnimals suspected of having heart disease did not show significant differences (p < .05) in terms of biometry or in relation to lipid profile and apolipoproteins A‐1 and B values; however, higher values of LDL and ApoB and ApoB/ApoA‐1 were observed in this group.ConclusionsThis study is the first to describe the lipid profile and apolipoprotein values in owl monkeys, and further work will be needed to better elucidate the worthiness of LDL, ApoB, and the ApoB/ApoA‐1 ratio in this primate species.
A model to determine at‐home restrictions for cats after treatment of hyperthyroidism with radioiodine
Authors: Peak K. , Alonzi C. , Gower L. , Walker D. , Johnson B. 
Published: August 2022

ObjectivesFelinehyperthyroidism is the most common endocrine disease of older cats and radioiodine is considered to be the gold standard treatment. Isolation periods following treatment vary depending on both individual treatment facilities and the relevant legislation of the country; therefore, there is no recognised standardised protocol defining the length of isolation. This work describes how our institution validated that its owner restrictions met dose constraints by using a model of iodine retention to calculate the required duration and nature of owner restrictions.Materials and MethodsThe retained radioactivity of cats at the point of discharge was used to simulate the radiation dose to owners in the 90 days following release. The model created was used to calculate the minimum duration of isolation for a range of administered activities and owner restrictions.ResultsUsing the model, it was found that when injected with the maximum dose used, 222 MBq radioiodine, it was possible to release cats after 14 days of isolation and keep owner doses below 0.30 mSv (whole‐body effective dose constraint for a single radiation source) with some restrictions. It was possible to release after 23 days with no restrictions.Clinical SignificanceThe present study provides clinicians with a consistent and verified method in which they can calculate the isolation periods for radioiodine‐treated cats.
A multi‐omics framework reveals strawberry flavor genes and their regulatory elements
Authors: Fan Zhen , Tieman Denise M. , Knapp Steven J. , Zerbe Philipp , Famula Randi , Barbey Christopher R. , Folta Kevin M. , Amadeu Rodrigo R. , Lee Manbo , Oh Youngjae , Lee Seonghee , Whitaker Vance M. 
Published: August 2022

Summary

Flavor is essential to consumer preference of foods and is an increasing focus of plant breeding programs. In fruit crops, identifying genes underlying volatile organic compounds has great promise to accelerate flavor improvement, but polyploidy and heterozygosity in many species have slowed progress.

Here we use octoploid cultivated strawberry to demonstrate how genomic heterozygosity, transcriptomic intricacy and fruit metabolomic diversity can be treated as strengths and leveraged to uncover fruit flavor genes and their regulatory elements.

Multi‐omics datasets were generated including an expression quantitative trait loci map with 196 diverse breeding lines, haplotype‐phased genomes of a highly‐flavored breeding selection, a genome‐wide structural variant map using five haplotypes, and volatile genome‐wide association study (GWAS) with > 300 individuals. Overlaying regulatory elements, structural variants and GWAS‐linked allele‐specific expression of numerous genes to variation in volatile compounds important to flavor. In one example, the functional role of anthranilate synthase alpha subunit 1 in methyl anthranilate biosynthesis was supported via fruit transient gene expression assays.

These results demonstrate a framework for flavor gene discovery in fruit crops and a pathway to molecular breeding of cultivars with complex and desirable flavor.
Aviation psychology: Applied methods and techniques, by Ioana V.Koglbauer, and SonjaBiede‐Straussberger (Eds.) Hogrefe Publishing, 2021, 168 pages, $45.00 paperback
Authors: Pearson Jonathan 
Published: August 2022

AbstractThe field of aviation consists of many different organizations, operations, and functions. Psychologists working in design and implementation, various aspects of aviation, have built up vast knowledge bases and techniques for best practices within their silos; however, there is no shared, common, knowledge base for these disparate fields within aviation. This book serves to provide an overview of psychological methods and tools used in various areas of research, implementation, and design within aviation fields.
The role of foreign body response in peri‐implantitis: What is the evidence?
Authors: Ivanovski Sašo , Bartold Peter Mark , Huang Yu‐Sheng 
Published: August 2022

AbstractHistorically, there has been broad consensus that osseointegration represents a homeostasis between a titanium dental implant and the surrounding bone, and that the crestal bone loss characteristic of peri‐implantitis is a plaque‐induced inflammatory process. However, this notion has been challenged over the past decade by proponents of a theory that considers osseointegration an inflammatory process characterized by a foreign body reaction and peri‐implant bone loss as an exacerbation of this inflammatory response. A key difference in these two schools of thought is the perception of the relative importance of dental plaque in the pathogenesis of crestal bone loss around implants, with obvious implications for treatment. This review investigates the evidence for a persistent foreign body reaction at osseointegrated dental implants and its possible role in crestal bone loss characteristic of peri‐implantitis. Further, the role of implant‐related material release within the surrounding tissue, particularly titanium particles and corrosion by‐products, in the establishment and progression in peri‐implantitis is explored. While it is acknowledged that these issues require further investigation, the available evidence suggests that osseointegration is a state of homeostasis between the titanium implant and surrounding tissues, with little evidence that a persistent foreign body reaction is responsible for peri‐implant bone loss after osseointegration is established. Further, there is a lack of evidence for a unidirectional causative role of corrosion by‐products and titanium particles as possible non–plaque related factors in the etiology of peri‐implantitis.
Putting Galileo in his Place: Geographical Origins and the Rhetoric of Scholarly Credibility☆
Authors: Post Anna‐Luna 
Published: August 2022

AbstractWhile in theory frowned upon, comments on the (regional) provenance of scholars frequently found their way into the scholarly debates of the Republic of Letters. This article uses early responses to Galileo Galilei's Sidereus Nuncius as a case study to explore various broader assumptions and associations underlying the use of such comments on provenance. Presenting a typology of these arguments, the article contends that provenance was used as a rather flexible marker of credibility, which allowed seventeenth‐century scholars to employ it to either advocate for, or against credibility. Scholars made full use of the rhetorical possibilities that origin‐based associations offered, demonstrating their flexible – if not to say opportunistic – attitude towards scholarly credibility in the process. This article furthermore investigates the motives Galileo's contemporaries had for invoking surreptitious boasts or slurs about provenance. In doing so, it illuminates a crucial mechanism informing the selection of specific arguments: competition on a personal, regional and national level.
Is the Omicron variant truly less virulent in solid organ transplant recipients?
Authors: Anjan Shweta , Khatri Akshay , Viotti Julia Bini , Cheung Teresa , Garcia Leopoldo A. Cordova , Simkins Jacques , Loebe Matthias , Phancao Anita , O'Brien Christopher B. , Sinha Neeraj , Ciancio Gaetano , Vianna Rodrigo M. , Andrews David , Abbo Lilian M. , Guerra Giselle , Natori Yoichiro 
Published: August 2022

AbstractSolid organ transplant (SOT) recipients are at high risk for severe disease with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. Emerging variants of concern have disproportionately affected this population. Data on severity and outcomes with the Omicron variant in SOT recipients are limited. Thus we conducted this single‐center, retrospective cohort study of SOT recipients diagnosed with SARS‐CoV‐2 infection from December 18, 2021 to January 18, 2022, when prevalence of the Omicron variant was more than 80%‐95% in the community. Univariate and multivariate logistic regression analysis was performed to identify risk factors for hospital admission. We identified 166 SOT patients: 112 (67.5%) kidney, 22 (13.3%) liver, 10 (6.0%) lung, seven (4.2%) heart, and 15 (9.0%) combined transplants. SARS‐CoV‐2 vaccine series was completed in 59 (35.5%) recipients. Ninety‐nine (59.6%) and 13 (7.8%) recipients received casirivimab/imdevimab and sotrovimab, respectively. Fifty‐three (32%) recipients required hospital admission, of which 19 (35.8%) required intensive care unit level of care. Median follow‐up was 50 (interquartile range, 25‐59) days, with mortality reported in six (3.6%) patients. Risk factors identified for hospital admission were African American race (p < .001, odds ratio [OR] 4.00, 95% confidence interval [CI] 1.84‐8.70), history of coronary artery disease (p = .031, OR 3.50, 95% CI 1.12‐10.87), and maintenance immunosuppression with corticosteroids (p = .048, OR 2.00, 95% CI 1.01‐4.00). In conclusion, contrary to that in the general population, we found a higher hospital admission rate in SOT recipients with omicron variant infection. Further studies to investigate the efficacy of newer treatments are necessary, even as outcomes continue to improve.
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The identification of the missing maternal genome of the allohexaploid camelina (Camelina sativa)
Authors: Mandáková Terezie , Lysak Martin A. 
Published: August 2022

SUMMARYHexaploid camelina (Camelina sativa; 2n = 6x = 40) is an important oilseed crop closely related to Arabidopsis. Compared to other polyploid crops, the origin of the three camelina subgenomes has begun to be unveiled only recently. While phylogenomic studies identified the diploid C. hispida (2n = 2x = 14) as the paternal genome of C. sativa, the maternal donor genome remained unknown. Because the chromosomes assigned to a putative maternal genome resembled those of diploid C. neglecta (2n = 12), a tetraploid C. neglecta‐like genome (2n = 4x = 26) was hypothesized to be the likely maternal ancestor of the hexaploid crop. Here we report the chromosome‐level structure of the predicted tetraploid Camelina genome identified among genotypes previously classified together as C. microcarpa and referred to here as C. intermedia. Detailed cytogenomic analysis of the tetraploid genome revealed high collinearity with two maternally inherited subgenomes of the hexaploid C. sativa. The identification of the missing donor tetraploid genome provides new insights into the reticulate evolutionary history of the Camelina polyploid complex and allows us to postulate a comprehensive evolutionary model for the genus. The herein elucidated origin of the C. sativa genome opens the door for subsequent genome modifications and resynthesis of the allohexaploid camelina genome.
Incidence and risk factors for intravenous immunoglobulin‐related hemolysis: A systematic review of clinical trial and real‐world populations
Authors: Cuesta Hillary , El Menyawi Ibrahim , Hubsch Alphonse , Hoefferer Liane , Mielke Orell , Gabriel Susie , Shebl Amgad 
Published: August 2022

AbstractBackgroundSevere hemolysis rarely occurs in patients receiving intravenous immunoglobulin (IVIG) therapy. A systematic review was performed to assess the incidence of IVIG‐related hemolysis and the impact of patient and product risk factors.Study Design and MethodsA systematic literature search for terms related to “IVIG products”, “hemolysis,” and “adverse events” was conducted in Embase for articles published between January 1, 2015, and May 31, 2021. Studies with no clinical datasets, no IVIG treatment, or where IVIG was used to treat hemolytic conditions were excluded. Of the 430 articles retrieved, 383 were excluded based on titles/abstracts and 14 were excluded after in‐depth review.ResultsIn total, 33 articles were analyzed and separated into observational studies (n = 16), clinical trials (n = 8), and case reports (n = 9). The incidence proportion for IVIG‐related hemolysis ranged from 0% to 19% in observational studies and 0%–21% in clinical trials. A higher incidence of IVIG‐related hemolysis was consistently reported in patients with blood groups A and AB. Hemolysis occurred more frequently in patients treated with IVIG for some conditions such as Kawasaki disease; however, this may be confounded by the high dose of IVIG therapy. IVIG‐related hemolysis incidence was lower in studies using IVIG products citing manufacturing processes to reduce isoagglutinin levels than products that did not.ConclusionThis analysis identified patient and product risk factors including blood group, IVIG dose, and IVIG manufacturing processes associated with elevated IVIG‐related hemolysis incidence.
Anatomic study of the canine bronchial tree using silicone casts, radiography, and CT
Authors: Scrivani Peter V. , Percival Aaron 
Published: August 2022

AbstractThis prospective anatomic study examined the canine lower airway lumen morphology by visual inspection, radiography, and CT of silicone casts of two dogs. Study results showed the lobar, bronchopulmonary segmental, and pulmonary lobular organization of the lungs grossly reflected in the hierarchically clustered branching structure of the bronchial tree. Study results also provided a basis for differentiating bronchial and bronchiolar patterns. Whereas images of silicone‐filled bronchial lumens produced large‐diameter, branching, tapering bands that radiated from the lung hilus, the pattern was different for small airways. Images of silicone‐filled bronchiole lumens produced small‐diameter, branching, tapering, nonradiating lines that created a speckled pattern resembling micronodules and, unexpectedly during radiography, small‐diameter rings with air‐filled lumens attributed to positive and negative summation shadows. Consequently, imaging patterns in dogs might be affected by pulmonary lobular morphology and might differentiate large and small airway diseases.
Avanzar al conocimiento científico: estado del arte del aprendizaje invertido
Authors: Araguz-Lara Virginia Nohemi , Acuña-Gamboa Luis Alan , Bonilla-Murillo Enrique 
Published: August 2022

Se revisa el estado del arte del aprendizaje invertido tomando como líneas de discusión las teorías, los diseños instruccionales y los modelos utilizados.Dicha revisión se realizó desde el método de análisis de contenido con apoyo de fichas de resumen, comentarios y de opinión. Los hallazgos indican que el diseño instructivo del modelo beneficia más a estudiantes con bajo rendimiento y que la configuración de la preparación previa influye significativamente en los procesos que ocurren en el aula. Se encuentran vacíos en el tema como la escasez de estudios longitudinales y a nivel institución, así como de diseño instruccional diferenciado y adaptativo.