bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated bioinformatic analysis of dysregulated microRNA‐mRNA co‐expression network in ovarian endometriosis
Authors: Liu Yong , Fan Linyuan , Jin Lingge , Lu Chang , Li Ting , Zhang Zhan , Xie Chengmao , Li Shenghui , Zhang Yudi , Ren Jian , Lu Dan 
Published: July 2022

AbstractIntroductionOvarian endometriosis is a frequently occurring gynecological disease with large socioeconomic impact. Accumulating evidence has suggested that aberrant miRNA‐mRNA interactions are involved in the pathogenesis and progression of ovarian endometriosis. This study aims to identify key miRNAs in ovarian endometriosis by using integrated bioinformatic analysis of a dysregulated miRNA‐mRNA co‐expression network.Material and methodsExpression profiling of miRNA and mRNA in three normal endometria and five pairs of ectopic/eutopic endometria from patients with ovarian endometriosis was determined by high‐throughput sequencing techniques. The data were then integrated with the public sequencing datasets (GSE105764 and GSE105765) using a non‐biased approach and a miRNA‐mRNA co‐expression regulatory network was constructed by in‐depth bioinformatic analysis.ResultsThe constructed miRNA‐mRNA network included 87 functionally DEMs, 482 target mRNAs and 1850 paired miRNA‐mRNA regulatory interactions. Specifically, five miRNAs (miR‐141‐3p, miR‐363‐3p, miR‐577, miR‐767‐5p, miR‐96‐5p) were gradually decreased and two miRNAs (miR‐493‐5p, miR‐592) were gradually increased from normal endometria to eutopic endometria, and then ectopic endometria tissues. Importantly, miR‐141‐3p, miR‐363‐3p and miR‐96‐5p belonged to the miR‐200 family, miR‐106a‐363 cluster and miR‐183/96/182 cluster, respectively. Their target mRNAs were mainly associated with cell adhesion, locomotion and binding, which are suggested to play vital regulatory roles in the pathogenesis of ovarian endometriosis.ConclusionsIntegrated bioinformatic analysis of the miRNA‐mRNA co‐expression network defines the crucial roles of the miR‐200 family, miR‐106a‐363 cluster and miR‐183/96/182 cluster in the pathogenesis of ovarian endometriosis. Further in‐depth functional studies are needed to unveil the molecular mechanisms of these miRNAs, and may provide clues for the optimization of therapeutic strategies for ovarian endometriosis.
The Report of the Van Fleet Missionas an intended blueprint for American grand strategy in the Asia‐Pacific during the early Cold War
Authors: Jo Kyu‐hyun 
Published: July 2022

AbstractTheVan Fleet Mission's Reporthas long received much attention in Korea and Japan as a controversial document stating the United States' position on the Dokdo/Takeshima dispute. However, linking theReportonly with Dokdo/Takeshima is missing the forest for the trees. TheVan Fleet Report's genuine importance with regard to the document's intention is that it was an intended blueprint for an American grand strategy in the Asia‐Pacific. Its central objective was to organize an anti‐communist bloc with Japan as its core and use it to counter the expansionist tendency of Chinese communism toward the Asia‐Pacific and strengthen Korea, Japan, and the Philippines military capabilities.
The horizontal S‐shaped relationship between corporate social responsibility and financial performance: The moderating effects of firm size and industry dynamism
Authors: Wang Kewen , Qiao Yuanbo 
Published: July 2022

AbstractThe relationship between corporate social responsibility (CSR) and corporate financial performance (CFP) is inconclusive. Building on CSR power and stakeholder power arguments, slack resource theory, and agency theory, this study constructs a three‐stage model to compare CSR benefits and costs. This comparison indicates a horizontal S‐shaped CSR‐CFP relationship. Using a sample including 27,628 firm‐year observations of Chinese listed firms from 2010 to 2020, we find evidence for this horizontal S‐shaped CSR‐CFP relationship, which reveals that the S curve is more pronounced for smaller firms and for firms operating in more dynamic industries. Our findings contribute to the CSR‐CFP literature, CSR power and stakeholder power arguments, and the evaluation of the moderating effects on the S curve. Accordingly, these findings highlight key implications for decision‐makers and policymakers.
Drug repositioning of tranilast to sensitize a cancer therapy by targeting cancer‐associated fibroblast
Authors: Ochi Kosuke , Suzawa Ken , Thu Yin Min , Takatsu Fumiaki , Tsudaka Shimpei , Zhu Yidan , Nakata Kentaro , Takeda Tatsuaki , Shien Kazuhiko , Yamamoto Hiromasa , Okazaki Mikio , Sugimoto Seiichiro , Shien Tadahiko , Okamoto Yoshiharu , Tomida Shuta , Toyooka Shinichi 
Published: July 2022

AbstractCancer‐associated fibroblasts (CAFs) are a major component of the tumor microenvironment that mediate resistance of cancer cells to anticancer drugs. Tranilast is an antiallergic drug that suppresses the release of cytokines from various inflammatory cells. In this study, we investigated the inhibitory effect of tranilast on the interactions between non–small cell lung cancer (NSCLC) cells and the CAFs in the tumor microenvironment. Three EGFR‐mutant NSCLC cell lines, two KRAS‐mutant cell lines, and three CAFs derived from NSCLC patients were used. To mimic the tumor microenvironment, the NSCLC cells were cocultured with the CAFs in vitro, and the molecular profiles and sensitivity to molecular targeted therapy were assessed. Crosstalk between NSCLC cells and CAFs induced multiple biological effects on the NSCLC cells both in vivo and in vitro, including activation of the STAT3 signaling pathway, promotion of xenograft tumor growth, induction of epithelial‐mesenchymal transition (EMT), and acquisition of resistance to molecular‐targeted therapy, including EGFR‐mutant NSCLC cells to osimertinib and of KRAS‐mutant NSCLC cells to selumetinib. Treatment with tranilast led to inhibition of IL‐6 secretion from the CAFs, which, in turn, resulted in inhibition of CAF‐induced phospho‐STAT3 upregulation. Tranilast also inhibited CAF‐induced EMT in the NSCLC cells. Finally, combined administration of tranilast with molecular‐targeted therapy reversed the CAF‐mediated resistance of the NSCLC cells to the molecular‐targeted drugs, both in vitro and in vivo. Our results showed that combined administration of tranilast with molecular‐targeted therapy is a possible new treatment strategy to overcome drug resistance caused by cancer‐CAF interaction.
The effectiveness of moral disengagement and social norms as anti‐bullying components: A randomized controlled trial
Authors: Tolmatcheff Chloé , Galand Benoit , Roskam Isabelle , Veenstra René 
Published: July 2022

AbstractThis three‐armed randomized controlled trial examined how moral disengagement and social norms account for change in bullying behavior and their potential as targets of anti‐bullying components within separate interventions among 1200 French‐speaking Belgian elementary students (48% boys, 9–12 year‐olds, 57 classes, nine schools) during 2018–2019 (no ethnicity data available). Mediation analysis revealed that students' moral disengagement successfully decreased (β = −.46), which, in turn, reduced both bullying (β = .33) and outsider behaviors (β = .20), and increased defending (β = −.10). Intervening on social norms decreased bullying (β = −.18), but not through the perceived injunctive class norm as intended. Guidelines to open the “black box” of anti‐bullying programs and determine the cost‐effectiveness ratio of their components are provided.
Protective role of TLR9‐induced macrophage/microglia phagocytosis after experimental intracerebral hemorrhage in mice
Authors: Wei Jialiang , Dai Shuhui , Pu Chen , Luo Peng , Yang Yuefan , Jiang Xiaofan , Li Xia , Lin Wei , Fei Zhou 
Published: July 2022

AbstractIntroductionIntracerebral hemorrhage (ICH) causes devastating morbidity and mortality, and studies have shown that the toxic components of hematomas play key roles in brain damage after ICH. Recent studies have found that TLR9 participates in regulating the phagocytosis of peripheral macrophages. The current study examined the role of TLR9 in macrophage/microglial (M/M) function after ICH.MethodsRAW264.7 (macrophage), BV2 (microglia), and HT22# (neurons) cell lines were transfected with lentivirus for TLR9 overexpression. Whole blood from C57BL/6 or EGFPTg/+ mice was infused for phagocytosis and injury experiments, and brusatol was used for the experiments. Intraperitoneal injection of the TLR9 agonist ODN1826 or control ODN2138 was performed on days 1, 3, 5, 7, and 28 after ICH to study the effects of TLR9 in mice. In addition, clodronate was coinjected in M/M elimination experiments. The brains were collected for histological and protein experiments at different time points after ICH induction. Cellular and histological methods were used to measure hematoma/iron residual, M/Ms variation, neural injury, and brain tissue loss. Behavioral tests were performed premodeling and on days 1, 3, 7, and 28 post‐ICH.ResultsOverexpression of TLR9 facilitated M/M phagocytosis and protected neurons from blood‐derived hazards in vitro. Furthermore, ODN1826 boosted M/M activation and phagocytic function, facilitated hematoma/iron resolution, reduced brain injury, and improved neurological function recovery in ICH mice, which were abolished by clodronate injection. The experimental results indicated that the Nrf2/CD204 pathway participated in TLR9‐induced M/M phagocytosis after ICH.ConclusionOur study suggests a protective role for TLR9‐enhanced M/M phagocytosis via the Nrf2/CD204 pathway after ICH. Our findings may serve as potential targets for ICH treatment.
The roles of polymer relaxation phenomena, aqueous dye solubility and the physical properties of water in the mechanism of adsorption of a disperse dye on poly(ethylene terephthalate) fibres: Part 3 physical properties of water and water‐derived fibre properties
Authors: Burkinshaw Stephen M. , Liu Kangsheng 
Published: July 2022

AbstractIn the absence of published information regarding the temperature dependency of water‐derived poly(ethylene terephthalate) fibre properties, the findings reported for the thermally regulated interactions between water and 100% amorphous poly(ethylene terephthalate) materials were interpreted from the perspective of the amorphous domains that reside within semi‐crystalline polyester textile fibres. This analysis suggests that the pronounced temperature dependent uptake of a commercial grade disperse dye on poly(ethylene terephthalate) fabric achieved during an aqueous dyeing process at temperatures between 30 and 130°C is the likely result of the combination of three separate, but inherently inter‐related, thermally activated phenomena, namely, polymer structural relaxation, in which polymer glass transition assumes a dominant role, dissolution of disperse dye in the aqueous dyebath, as well as various water–fibre interactions, in the guise of water sorption, water molecule diffusivity, water‐induced swelling and water‐induced plasticisation. Although thermally regulated macromolecular relaxation processes adopt the principal role in dye uptake, temperature dependent dye solubility and water‐derived fibre properties nevertheless likely provide crucially important supportive roles.
Separate and combined effect of visit‐to‐visit glycaemic variability and mean fasting blood glucose level on all‐cause mortality in patients with type 2 diabetes: A population‐based cohort study
Authors: Liu Yahang , Xu Huilin , Li Jun , Yang Yating , Zhang Jie , Liu Xiaoqin , Li Jiong , Yu Yongfu , Qin Guoyou 
Published: July 2022

AbstractAimsTo assess the independent and combined impacts of visit‐to‐visit fasting blood glucose variability (VVV‐FBG) and mean fasting blood glucose level (M‐FBG) on all‐cause mortality.Materials and methodsThis prospective cohort study included 48 843 Chinese patients with type 2 diabetes. Cox proportional hazards regression models were used to calculate hazard ratios (HRs) and 95% confidence intervals (CIs) to evaluate the association of VVV‐FBG and M‐FBG with all‐cause mortality. The potential nonlinear associations were examined using restricted cubic splines, and additive interaction was evaluated using relative excess risk due to interaction (RERI). Cox generalized additive models (CGAMs) and bivariate response surface models were further used to assess the combined effects of VVV‐FBG and M‐FBG.ResultsA total of 4087 deaths were observed during a median follow‐up of 6.99 years. Compared with patients with values at the 5th percentile of average real variability (ARV) and M‐FBG, we observed a 23% and 38% increased risk of premature deaths among those with values at the 95th percentile of ARV (HR 1.23, 95% CI 1.10, 1.37) and M‐FBG (HR 1.38, 95% CI 1.26, 1.51), respectively. The interaction between glycaemic variability (ARV) and M‐FBG was significant on both the additive scale (RERI 0.80 [0.29, 1.32]) and the multiplicative scale (HR 1.90 [1.10, 3.28]). High VVV‐FBG and high M‐FBG conferred the highest risk of all‐cause mortality (HR 1.89, 95% CI 1.64, 2.17), compared to low VVV‐FBG and low M‐FBG. The CGAMs showed significant synergistic effects between glycaemic variability and M‐FBG (P < 0.05). Moreover, a bivariate surface plot showed that risk of death increased more rapidly in type 2 diabetes patients with lower M‐FBG combined with lower VVV‐FBG.ConclusionsThe coexistence of high glycaemic variability and high glucose level might exacerbate the independent risk of premature mortality in type 2 diabetes patients, highlighting the importance of achieving normal and stable glucose levels simultaneously in the management of glucose.
Post‐fire recovery of soil microbial functions is promoted by plant growth
Authors: Garcia‐Pausas Jordi , Romanyà Joan , Casals Pere 
Published: July 2022

AbstractForest fires can alter the biological properties of soils. There is increasing evidence that fires cause a shift in soil microbial communities, which play a central role in forest carbon and nutrient cycling. In this study, we evaluate the effect of soil heating on soil microbial functions. We hypothesised that fire reduces the catabolic functional diversity of soil, and that post‐fire plant growth enhances its recovery. To test this, we experimentally heated a forest soil at 200°C (T200) or 450°C (T450). Heated and unheated soils were then incubated in tubs with or without live grass (Lolium perenne L.). We determined the functional profiles by measuring the substrate‐induced respiration (SIR) using the Microresp™ technique and analysed nutrient availability at the end of the incubation. At both temperatures, soil heating altered the respiration responses to substrate additions and the catabolic functional diversity of soils. Functional diversity was initially reduced in T200 soils but recovered at the end of the incubation. In contrast, T450 soils initially maintained the catabolic functional diversity, but decreased at the end of the incubation. Heating‐induced nutrient availability stimulated the growth of grass, which in turn increased the response to several substrates and increased the functional diversity to values similar to the unheated controls. Our results suggest that fire‐driven alteration of soil microbial communities has consequences at a functional level, and that the recovery of plant communities enhances the recovery of soil microbial functions.Highlights
Soil experimental heating altered microbial functions and reduced soil functional diversity.
Soil heating also increased nutrient availability, enhancing plant growth.
Growth of plants promoted the recovery of soil functional diversity.
Post‐fire recovery of functional diversity may be related to the recovery of photosynthetic tissues.
Effects of foliar spraying of selenate at different time points on selenium concentration in wheat grains during grain filling period
Authors: Li Qiannan , Chen Menglin , Li Xinxin , Wang Youkai , Zhu Qing , Gao Songna , Wang Junpei , Wang Ying , Yu Feiyan , Wang Xugang , Huo Ke , Zhang Lianhe 
Published: July 2022

AbstractFoliar spraying of selenium (Se) during the wheat grain filling period can effectively increase grain Se concentration. However, it is unclear when foliar spraying Se can efficiently increase grain Se concentration. In this study, 0, 18.90, 37.80 and 56.70 g/ha of selenate were sprayed on the leaf blades on the 2nd and 9th day after grain filling, respectively, to investigate the effect of different spraying time points on Se concentration in aerial parts, especially the grains. The results indicated that Se concentrations in aerial parts increased with selenate levels at different time points. On the 7th day after spraying Se, Se concentrations in leaf blades, leaf sheaths, nodes, internodes and cobs sprayed with Se on the 2nd day after grain filling were significantly higher than those in corresponding parts sprayed with Se on the 9th day (p < 0.05). However, the Se concentrations of aerial parts on the 14th day after spraying Se and at grain maturity on the 2nd day after grain filling were significantly lower than those on the 7th day after spraying Se on the 9th day (p < 0.05). Furthermore, on the 7th day and 14th day after spraying Se and at grain maturity, grain Se concentrations sprayed with Se on the 2nd day after grain filling were significantly lower than those sprayed with Se on the 9th day (p < 0.05). These results suggested that spraying Se on the 9th day after grain filling was beneficial for increasing the Se concentration in aerial parts, especially grains. This study provides a theoretical basis for efficiently enhancing grain Se concentration and producing Se‐enriched bran and straw, which is of great significance for improving Se intake of livestock, enhancing livestock immunity, and promoting the development of animal husbandry.
Histopathological risk factors for malignancy in dermatomyositis
Authors: Pruessmann Wiebke , Kleinheinz Andreas , Zillikens Detlef , Rose Christian 
Published: July 2022

AimsTo identify possible histopathological risk factors for malignancy in skin biopsies of dermatomyositis patients.Methods and resultsWe analysed clinical metadata and studied 30 skin biopsies of 11 patients with and 12 patients without associated malignancy, who were treated in one secondary and one tertiary German medical centre between 2009 and 2022 and fulfilling the EULAR/ACR classification criteria for dermatomyositis. Specimens were categorized by malignancy status and evaluated based on haematoxylin and eosin (H&E), periodic acid Schiff (PAS), Alcian Blue, and anti‐CD123 immunohistochemistry stains. After correcting for multiple testing, biopsies of patients with cancer exhibited more severe basement membrane thickening (P < 0.05) and pigment incontinence (P < 0.05) compared to patients without tumour burden. Patients with numerous subepidermal melanophages had a more than 5‐times increased odds ratio to suffer from an internal malignancy (odds ratio [OR] 5.3, 95% confidence interval [CI] 1.3–54.2, P < 0.05). Furthermore, specimens of the malignancy group presented a distinct superficial distribution pattern of CD123+ plasmacytoid dendritic cells (PDCs, P < 0.01). Extravascular eosinophils were absent in all cases.ConclusionSeverity of basement membrane thickening, extent of pigment incontinence, and superficial distribution pattern of CD123+ PDCs could serve as useful histopathological indicators of risk for malignancy in dermatomyositis.
Recovery of the phenolic compounds from artificial cultivated Sanghuangporus vaninii using a green method and biological properties of phenolic extract in vitro
Authors: Zhang Yangyang , Lv Guoying , Song Tingting , Chen Chun , Zhang Zuofa , Cai Weiming 
Published: July 2022

SummaryModern research has demonstrated that Sanghuangporus vaninii (S. vaninii) has significant therapeutic value, and phenolic compounds are one of its most active ingredients. In this study, deep eutectic solvents (DES) were used to extract the phenolic components from S. vaninii using response surface methodology. The optimal conditions were obtained with a water content of 20%, a material‐to‐liquid ratio of 1:60 g mL−1, an extraction temperature of 70°C and an extraction time of 34 min. Under these conditions, the total phenolic content reached 9.71 ± 0.69 mg g−1, which was higher than the conventional method (4.23 ± 0.44 mg g−1). The phenolic extract was then purified by HPD‐100, and the purified phenolic extracts exhibited distinct antioxidant, antibacterial and hypoglycaemic activities. Twenty‐five phenolic compounds were identified using UPLC‐Triple‐TOF‐MS/MS. These data implied that the integrated action of the identified phenolic compounds had a decisive influence on the biological activity of the extracts, and this study furnished the basis for the utilisation of S. vaninii.
Nutritional composition and in vitro starch digestibility of Banku flour processed from cassava (Manihot esculenta Crantz) root and quality protein maize grains
Authors: Ogundele Gabriel F. , Adebayo Tesleem K. , Adeyanju Adeyemi A. , Bamidele Oluwaseun P. 
Published: July 2022

SummaryA significant public health problem in developing countries is protein energy malnutrition. The current study looked at the effect of incorporating high‐quality protein maize flour into blends of cassava root flour on nutrient composition, functional properties and sensory attributes of Banku flour. The Banku flour's proximate composition, functional properties, pasting qualities, in vitro starch digestibility, as well as sensory characteristics, were determined. The outcomes demonstrated that the addition of quality protein maize enhanced the protein content (from 5.96% to 13.11%), water absorption capacity (from 98.32% to 164.23%) and swelling capacity from (46.47% to 95.65%) of the flour. Also, peak viscosity, breakdown viscosity and final viscosity increased by 27.6%, 25% and 61.2 respectively. A rise in resistant starch and a decline in rapidly digestible starch were both observed in the in vitro starch digestibility of the Banku flour formulation that contained high‐quality protein maize flour. Overall, the Banku flour produced from a blend of high‐quality protein maize flour and cassava root flour outperformed the control sample. Consequently, Banku's nutritional quality can be enhanced by using high‐quality protein maize.
Reduction of the refining‐conching time of Peruvian dark chocolate: a case study
Authors: Valverde‐Ayllon Maria C. , Chire‐Fajardo Gabriela Cristina , Ureña‐Peralta Milber Oswaldo 
Published: July 2022

SummaryThe refining‐conching stage is crucial in the chocolate manufacturing process for the development of sensory and quality characteristics. However, this stage requires the longest amount of operation time. Thus, the objective of this research was to reduce the refining‐conching time of dark chocolate in a Macintyre refiner/conche in a Peruvian medium‐scale chocolate company. Physical properties of chocolate samples as particle size (by micrometer (PSM) and image analyis (PSIA)), flow behaviour (plastic viscosity (PV) and yield stress (YS)) were determined every 2 h during a total refining‐conching operation time of 20 h. After 12 h, no significant differences were found in those physical properties. Moisture content and a particle size distribution (PSD) percentile DV50 were also measured. Moisture content was measured at the beginning (0 h), at the end of the conventional (20 h) and at the reduced processing time (12 h). Additionally, a sensory evaluation was performed by a trained panel to compare chocolates obtained at a reduced (12 h) and conventional (20 h) refining‐conching times, and no significant differences were found (P < 0.05). These results represented a total reduction time of 8 h for the refining‐conching of dark chocolate.