bluuebunny/crossref_metadata_2025_split huggingface dataset:
Water Oxidation by a Neoteric Dinuclear Mn(II) Electrocatalyst in Aqueous Medium
Authors: Raj Manaswini , Padhi Sumanta Kumar 
Published: May 2022

AbstractThe employment of manganese complexes towards electrocatalytic water oxidation has gained immense interest among the scientific communities. In this present study, the reaction of Mn(CH3COO)2⋅4H2O with the novel ligand (2‐([2,2′:6′,2“‐terpyridin]‐4′‐yl)quinoline‐8‐ol) (8hq‐tpy) affords a dinuclear manganese complex, [MnII(8hq‐tpy)(H2O)]2(PF6)2 (Mn 1). Various spectroscopic techniques have been implemented for characterization purposes, and Mn 1 is employed as electrocatalysts towards water oxidation catalysis in the aqueous medium. Electrocatalytic responses in the aqueous medium at pH 6.0 lead to the electrodeposition of heterogeneous species (MnOX) which enables the oxidation event at an applied potential of 1.24 V vs. NHE with O2 liberation of 50.2 μmol/L. The XPS analysis performed after executing bulk electrolysis, provide evidence for the generation of MnIV=O as the active species formed by the catalyst precursor complex Mn 1, contributing to O2 evolution during the electrocatalytic water oxidation process.
Experimental modeling and optimization of melanin production by Aureobasidium pullulansNBRC 100716 in carrot peel extract
Authors: Müjdeci Gamze Nur 
Published: May 2022

AbstractThe objective of this study was to optimize the fermentation parameters by using Response Surface Methodology for increasing melanin production in carrot peel extract by Aureobasidium pullulans NBRC 100716. The optimum conditions for melanin production by A. pullulans NBRC 100716 in carrot peel extract were determined as; the initial pH of the fermentation medium: 5.9, temperature: 29.9°C, agitation rate: 120 rpm, and fermentation time: 15 days. The estimated intracellular (Y1), extracellular (Y2), and total (Y3) melanin concentrations in optimum conditions were 2.44 ± 0.05, 1.95 ± 0.47, and 4.22 ± 0.74 g/L, respectively. As a result of confirmation experiments, the observed values of Y1, Y2, and Y3 were 2.23, 1.83, and 4.06 g/L, respectively. The optimization of melanin production conditions by A. pullulans NBRC 100716 caused an almost one‐fold increase in total melanin concentration. Besides, using carrot peel extract as an inexpensive substrate source for the production of high value‐added melanin, the optimization of the fermentation conditions is beneficial for the production of this product.
Data‐driven modelling of joint debris flow release susceptibility and connectivity
Authors: Steger Stefan , Scorpio Vittoria , Comiti Francesco , Cavalli Marco 
Published: May 2022

AbstractIn mountain basins, sediment supply to the fluvial system occurs mainly through episodic geomorphic processes—such as debris flows and other landslide types—whose effectiveness is strongly influenced by the structural connectivity within a catchment. This paper presents a novel data‐driven approach to identify and map areas that are simultaneously susceptible to debris flow initiation and structurally connected to the main channel network (i.e. relevant sediment source areas for predicting and mitigating flood hazards in the river channels). The presented approach comprises: (i) the visual interpretation, delineation, mapping and classification of event‐specific connected and disconnected debris flow areas in three catchments of the Italian Alps; (ii) the development of data‐driven debris flow release susceptibility models that are combined with quantitatively classified index of connectivity (IC) maps; and (iii) a thorough evaluation of the approach, including an assessment of its spatial transferability across the catchments. The main results show: (i) quantitative IC thresholds to discriminate connected from disconnected debris flow release areas; (ii) statistically well‐performing and geomorphically plausible debris flow release susceptibility models for the three basins; (iii) diverse joint debris flow connectivity–susceptibility maps that allow identifying zones which are differently relevant in terms of debris flow connectivity. This work also highlights the spatial transferability of the approach, associated benefits and potential drawbacks, as well as the utmost importance of a thorough combined quantitative and qualitative (i.e. geomorphic plausibility) evaluation of the underlying results. The proposed approach is rather simple and requires few basic input data, and can therefore be applied over vast areas (e.g. to support regional‐scale hazard assessments or sediment management plans).
Strong selective environments determine evolutionary outcome in time-dependent fitness seascapes
Authors: Cairns Johannes , Borse Florian , Mononen Tommi , Hiltunen Teppo , Mustonen Ville 
Published: May 2022

AbstractThe impact of fitness landscape features on evolutionary outcomes has attracted considerable interest in recent decades. However, evolution often occurs under time-dependent selection in so-called fitness seascapes where the landscape is under flux. Fitness seascapes are an inherent feature of natural environments, where the landscape changes owing both to the intrinsic fitness consequences of previous adaptations and extrinsic changes in selected traits caused by new environments. The complexity of such seascapes may curb the predictability of evolution. However, empirical efforts to test this question using a comprehensive set of regimes are lacking. Here, we employed an in vitro microbial model system to investigate differences in evolutionary outcomes between time-invariant and time-dependent environments, including all possible temporal permutations, with three subinhibitory antimicrobials and a viral parasite (phage) as selective agents. Expectedly, time-invariant environments caused stronger directional selection for resistances compared to time-dependent environments. Intriguingly, however, multidrug resistance outcomes in both cases were largely driven by two strong selective agents (rifampicin and phage) out of four agents in total. These agents either caused cross-resistance or obscured the phenotypic effect of other resistance mutations, modulating the evolutionary outcome overall in time-invariant environments and as a function of exposure epoch in time-dependent environments. This suggests that identifying strong selective agents and their pleiotropic effects is critical for predicting evolution in fitness seascapes, with ramifications for evolutionarily informed strategies to mitigate drug resistance evolution.
Directly pricing VIX futures with observable dynamic jumps based on high‐frequency VIX
Authors: Jiang Gongyue , Qiao Gaoxiu , Ma Feng , Wang Lu 
Published: May 2022

AbstractThis paper proposes to study volatility index (VIX) futures pricing by directly modeling the logarithmic VIX while incorporating observable dynamic jumps of the VIX, which are derived based on VIX high‐frequency data. The impacts of several different interday and intraday jump tests for VIX futures prices are investigated. We obtain the analytical expression by deducing the forward iteration relations of the lagged logarithm VIX, as well as the conditional variance and jump intensity, and use the maximum likelihood method to estimate the parameters under the risk‐neutral measure. The empirical results prove the superiority of our newly proposed model (especially the model based on the LM jump test), which indicates that considering the heteroscedasticity effect of conditional variance, introducing VIX high‐frequency data information and separating realized jump variation from the realized variance are very important to obtain much more accurate VIX futures pricing.
A club model of nature-smart agriculture for biodiversity, climate, and productivity enhancements
Authors: Omer Amani 
Published: May 2022

Abstract
This article considers horizontal and vertical intensification interactions between environmental conservation and agricultural production to investigate a top–down partnership in agriculture. It addresses the issues underlying the challenge of food security. It explores a nature-based landscape approach to agriculture management that simultaneously ensures food security and safeguards the natural environment. The economic theory of clubs is applied to extend farm-level sustainable agricultural intensification and conservation practices to encompass interconnected activities within the entire landscape. A theoretical ecological-economic club model is used to explore how biodiversity and climate-related environmental goods and agricultural products are coproduced by private producers in agroecosystems of any spatial scale. The model considers any given agroecosystem as a club producing two goods: private economic output and public ecological output. It also proposes a dual environmental incentive approach to promote participants' individual and collaborative efforts. The model derives (a) the social conditions for the optimal application and allocation of resources for producing sustainable agricultural and ecological outputs and (b) the producer's incentive conditions for adequate levels of individual and collaborative conservation activities. Thus, the study identifies conditions that optimize horizontal and vertical agri-environmental farming intensification while preventing crowding out voluntary conservation activities. The model presented here can set a theoretical “benchmark” model to address real-life deviations comparatively. A practical proposition derived from the analysis points to the possibility that farm-scale conservation (of biodiversity and climate-related attributes), when extended to the landscape level, can optimally enhance the interconnected goals of agri-environmental farming. Thus, a data-driving methodology is described to outline a practical approach to the agricultural transition toward landscape conservation, a practical guide for policymakers and regulators who need to develop and provide tailor-made support to agricultural producer members of the club. Integr Environ Assess Manag 2023;19:412–421. © 2022 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
Dysbiosis of gut microbiota and intestinal damage in mice induced by a single intravenous exposure to CdTe quantum dots at low concentration
Authors: Li Xiaohui , Wei Huan , Hu Yun , Lv Yangfan , Weng Lixing , Teng Zhaogang , Yuwen Lihui , Wang Lianhui 
Published: May 2022

AbstractAlthough quantum dots (QDs) have shown great potential for various biomedical applications, their potential toxicity still needs to be comprehensively investigated. Previous studies showed that intravenous exposure of CdTe QDs at low concentration did not lead to obvious in vivo toxicity in the long term. However, the influence of CdTe QDs on the gut microbiota and the intestine is still unknown. Here, we explored whether single intravenous injection of CdTe QDs at low concentration can affect the gut microbiota and intestine of mice in short term. The results showed that CdTe QDs caused an imbalance of gut microbiota, especially the rapid increase in Lactobacillus on day 1 post‐treatment. Meanwhile, the intestine exhibited the promotion of oxidative stress, inflammatory response, and hemorrhaging on days 5 and 15. These results demonstrate that the gut microbiota and the intestine are very sensitive to the toxicity of low‐concentration CdTe QDs. This study provides further insight and method for the biosafety evaluation of nanomaterials.
MALT1 positively relates to Th17 cells, inflammation/activity degree, and its decrement along with treatment reflects TNF inhibitor response in ankylosing spondylitis patients
Authors: Yuan Jie , Xiang Lei , Wang Feng , Zhang Lin , Liu Gaozhan , Chang Xiuli , Zhang Anbing , Tao Ying 
Published: May 2022

AbstractBackgroundMucosa‐associated lymphoid tissue lymphoma translocation protein 1 (MALT1) facilitates CD4+ T‐cell differentiation, immune response, inflammation, and osteoclastogenesis. This study aimed to explore the relation between MALT1 and treatment efficacy to tumor necrosis factor inhibitor (TNFi) in ankylosing spondylitis (AS) patients.MethodsThis study recruited 73 AS patients underwent adalimumab treatment. Peripheral blood mononuclear cell (PBMC) was obtained at Week (W) 0, W4, W8, and W12 after treatment initiation; then, MALT1 was measured using RT‐qPCR. Furthermore, PBMC and serum at W0 were proposed to flow cytometry and ELISA for Th1 cells, Th17 cells, IFN‐γ, and IL‐17A levels measurement. Besides, 20 osteoarthritis patients and 20 healthy controls (HCs) were enrolled to detect MALT1.ResultsMucosa‐associated lymphoid tissue lymphoma translocation protein 1 expression was higher in AS patients compared with HCs (p < 0.001) and osteoarthritis patients (p < 0.001). Besides, MALT1 expression was positively linked with CRP (p = 0.002), BASDAI (p = 0.026), PGADA (p = 0.040), ASDASCRP (p = 0.028), Th17 cells (p = 0.020), and IL‐17A (p = 0.017) in AS patients, but did not relate to other clinical features, Th1 cells or IFN‐γ (all p>0.050). MALT1 was decreased along with treatment only in AS patients with ASAS40 response (p < 0.001), but not in those without ASAS40 response (p = 0.064). Notably, MALT1 expression was of no difference at W0 (p = 0.328), W4 (p = 0.280), and W8 (p = 0.080), but lower at W12 (p = 0.028) in AS patients with ASAS40 response compared with those without ASAS40 response.ConclusionMucosa‐associated lymphoid tissue lymphoma translocation protein 1 positively correlates with Th17 cells, inflammatory, and activity degree; meanwhile, its decrement along with treatment reflects the response to TNF inhibitor in AS patients.
Correlation of small nucleolar RNA host gene 16 with acute respiratory distress syndrome occurrence and prognosis in sepsis patients
Authors: Zhang Chengju , Huang Qinghe , He Fuyun 
Published: May 2022

AbstractBackgroundLong noncoding RNA small nucleolar RNA host gene 16 (lnc‐SNHG16) regulates sepsis‐induced acute lung injury and inflammation, which is involved in the pathophysiology of acute respiratory distress syndrome (ARDS). The present study intended to explore the role of lnc‐SNHG16 as a potential biomarker indicating ARDS risk, disease severity, inflammation, and mortality in sepsis.MethodsPeripheral blood mononuclear cell (PBMC) samples were collected from 160 sepsis patients within 24 hours after admission and 30 healthy controls (HCs). Then, lnc‐SNHG16 in PBMCs was detected by reverse transcription‐quantitative polymerase chain reaction. Sepsis patients were followed up until death or up to 28 days.Resultslnc‐SNHG16 was declined in sepsis patients compared with HCs (p < 0.001). The incidence of ARDS was 27.5% among sepsis patients; meanwhile, sepsis patients with ARDS had higher mortality than those without ARDS (p < 0.001). Furthermore, lnc‐SNHG16 was declined in sepsis patients with ARDS compared to those without ARDS (p < 0.001); besides, higher lnc‐SNHG16 was independently correlated with declined ARDS occurrence in sepsis patients (p = 0.001), while primary respiratory infection and higher CRP were independently correlated with elevated ARDS occurrence in sepsis patients (both p < 0.05). Moreover, a negative correlation was found in lnc‐SNHG16 with history of diabetes, history of chronic obstructive pulmonary disease, and APACHE II and SOFA scores (all p < 0.05). Additionally, lnc‐SNHG16 was declined in sepsis deaths compared with survivors (p = 0.002), while it was not independently linked with sepsis mortality.Conclusionlnc‐SNHG16 correlates with lower ARDS occurrence and better prognosis in sepsis patients.
Adapting narrative exposure therapy with a tribal community: A community‐based approach
Authors: Bedard‐Gilligan Michele , Kaysen Debra , Cordero Rebeca Marin , Huh David , Walker Denise , Kaiser‐Schauer Elisabeth , Robjant Katy , Saluskin Kathy , Pearson Cynthia 
Published: May 2022

AbstractObjectiveAmerican Indian (AI) individuals are at increased risk for present‐day trauma exposure and associated negative outcomes, as well as ongoing effects of intergenerational trauma exposure and adversity. However, few empirically supported treatments exist that are specifically tailored and/or tested with AI communities. This study describes the process of selecting, adapting, and implementing narrative exposure therapy (NET) with an AI community.MethodsA community and academic partnership was formed and worked together to make culturally mindful changes to NET to best fit the needs of the community. The partnership incorporated community leaders/Elders (n = 7), providers (n = 11), and participants seeking treatment (n = 50) to implement an iterative process of adapting and implementing the adapted form of NET.ResultsKey adaptions included addressing historical and intergenerational trauma, greater protections for confidentiality in a small community, and incorporation of cultural customs and traditions. Overall, the adapted form of NET was favorably received by the participants, and the implementation appeared to be feasible, with improved retention over past trials of adapted trauma‐focused treatments with this community and with highly positive satisfaction ratings and feedback.ConclusionsNET was shown to be an appropriate approach for this AI community and should be considered as a treatment option for other AI communities. Future work should consider strategies outlined in this adaption as well as following a similar process for working with AI communities to implement culturally appropriate interventions for trauma‐related symptoms.
Interleukin‐1β activated c‐FOS transcription factor binds preferentially to a specific allele of the matrix metalloproteinase‐13 promoter and increases susceptibility to endometriosis
Authors: Pandit Anuradha , Das Mahapatra Pramathes , Saha Priyanka , Srivastava Amit Kumar , Swarnakar Snehasikta 
Published: May 2022

AbstractEndometriosis is a benign gynecological condition characterized by increased growth, inflammation, invasion, and angiogenesis, partly regulated by a class of enzymes called matrix metalloproteinases (MMPs). The importance of a few MMPs, e.g., MMP‐9, ‐3, and ‐7 has been studied in endometriosis progression. Although MMP‐13 plays an essential role in bone regeneration and cancer, no report has been found on the part of MMP‐13 and endometriosis progression. We found the upregulation of MMP‐13 expression and activity in patients having endometriosis in the eastern Indian population. In addition, the −77A/G polymorphism of the MMP13 promoter (rs: 2252070) is associated with regulating transcription and subsequent susceptibility to disease. In eastern Indian case‐control groups, the effect of the −77A/G single‐nucleotide polymorphism on MMP13 promoter activity and its relationship with endometriosis susceptibility was studied. The AG genotype was shown to be more predisposed to endometriosis risk than the GG genotype (p: 0.02; odds ratio [OR]: 1.65, 95% confidence interval [CI]: 1.10–2.49), also AG genotype was more frequent in late‐stage patients compared to early‐stage (p: 0.03, OR: 2.0, 95% CI: 1.09–3.66). Furthermore, the MMP13 gene levels were greater in AA compared to GG individuals. Additionally, MMP13 promoter‐reporter experiments in cultured endometrial epithelial cells and in silico analyses both demonstrated increased transcriptional activity near the G to A transition under basal/IL‐1β ‐induced/c‐FOS overexpressed condition. Overall, c‐FOS tighter binding to the A allele‐carrying promoter enhances MMP13 transcription, which is further amplified by IL‐1β due to increased c‐FOS phosphorylation, promoting MMP‐13 production and endometriosis risk.
A gain‐of‐function mutation in the ITPR1 gating domain causes male infertility in mice
Authors: Sun Bo , Ni Mingke , Tian Shanshan , Guo Wenting , Cai Shitian , Sondergaard Mads T. , Chen Yongxiang , Mu Yongxin , Estillore John P. , Wang Ruiwu , Chen Ju , Overgaard Michael T. , Fill Michael , Ramos‐Franco Josefina , Nyegaard Mette , Wayne Chen Sui Rong 
Published: May 2022

AbstractInositol 1,4,5‐trisphosphate receptor 1 (ITPR1) is an intracellular Ca2+ release channel critical for numerous cellular processes. Despite its ubiquitous physiological significance, ITPR1 mutations have thus far been linked to primarily movement disorders. Surprisingly, most disease‐associated ITPR1 mutations generate a loss of function. This leaves our understanding of ITPR1‐associated pathology oddly one‐sided, as little is known about the pathological consequences of ITPR1 gain of function (GOF). To this end, we generated an ITPR1 gating domain mutation (D2594K) that substantially enhanced the inositol trisphosphate (IP3)‐sensitivity of ITPR1, and a mouse model expressing this ITPR1‐D2594K+/− GOF mutation. We found that heterozygous ITPR1‐D2594K+/− mutant mice exhibited male infertility, azoospermia, and acrosome loss. Furthermore, we functionally characterized a human ITPR1 variant V494I identified in the UK Biobank database as potentially associated with disorders of the testis. We found that the ITPR1‐V494I variant significantly enhanced IP3‐induced Ca2+ release in HEK293 cells. Thus, ITPR1 hyperactivity may increase the risk of testicular dysfunction.
Interaction between mesenchymal stem cells and myoblasts in the context of facioscapulohumeral muscular dystrophy contributes to the disease phenotype
Authors: Kiseleva Ekaterina , Serbina Olesya , Karpukhina Anna , Mouly Vincent , Vassetzky Yegor S. 
Published: May 2022

AbstractFacioscapulohumeral muscular dystrophy (FSHD) is a genetic disease associated with ectopic expression of the DUX4 gene in skeletal muscle. Muscle degeneration in FSHD is accompanied by muscle tissue replacement with fat and connective tissue. Expression of DUX4 in myoblasts stimulates mesenchymal stem cells (MSC) migration via the CXCR4‐CXCL12 axis. MSCs participate in adipose and connective tissue formation and can contribute to fibrosis. Here we studied the interaction between myoblasts and MSCs and the consequences of this interaction in the FSHD context. We used cell motility assays and coculture of MSCs with myoblasts to study their mutual effects on cell migration, differentiation, proliferation, and extracellular matrix formation. The growth medium conditioned by FSHD myoblasts stimulated MSCs migration 1.6‐fold (p < 0.04) compared to nonconditioned medium. Blocking the CXCL12‐CXCR4 axis with the CXCR4 inhibitor (AMD3100) or neutralizing antibodies to CXCL12 abolished this effect. FSHD myoblasts stimulated MSC proliferation 1.5−2 times (p < 0.05) compared to control myoblasts, while the presence of MSCs impaired myoblast differentiation. Under inflammatory conditions, medium conditioned by FSHD myoblasts stimulated collagen secretion by MSCs 2.2‐fold as compared to the nonconditioned medium, p < 0.03. FSHD myoblasts attract MSCs via the CXCL12‐CXCR4 axis, stimulate MSC proliferation and collagen secretion by MSCs. Interaction between MSCs and FSHD myoblasts accounts for several important aspects of FSHD pathophysiology. The CXCL12‐CXCR4 axis may serve as a potential target to improve the state of the diseased muscles.