bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polydopamine-assisted in situ growth of AgNPs on face masks for the detection of pesticide based on surface-enhanced Raman scattering spectroscopy
Authors: Wang Tongtong , Zhang Qijia , Li Jia , Xu Guangda , Guo Na , Song Peng , Xia Lixin 
Published: April 2022

Abstract
Thiram is used as a fungicide and insecticide in agriculture to prevent white rot and anthracnose, but it also produces certain pesticide residues that endanger human health. Polydopamine (PDA) is an adhesive polymer that functionalizes almost all chemical materials, having been inspired by the adhesive properties of catechol and amines in mussel adhesive protein. In this work, we have fabricated a highly sensitive face mask (FM)-based surface-enhanced Raman scattering (SERS) substrate by depositing silver nanoparticles (AgNPs), using a PDA layer as an interface to promote adhesion. Face masks are currently ubiquitous, and are simple and easy to obtain, and this approach can be regarded as waste utilization. FM/PDA/AgNPs can detect Nile Blue A (NBA) probe molecules at concentrations as low as 1.0 × 10− 9 M, and permit measurements with sensitivity and uniformity. The Raman substrate can be used to swab the surface of fruit to detect thiram at concentrations as low as 1.0 × 10− 7 M, and the reproducibility has been assessed. The simple utilization of this flexible SERS substrate holds promise for its practical application in the field of pesticide control.
Research on the configuration paths of Sustainable Marine Eco-Efficiency: Based on fuzzy-set qualitative comparative analysis of 11 coastal areas in China
Authors: Zhenyu Huang 
Published: April 2022

Abstract
Studying the sustainable configuration path of marine ecological efficiency (MEE) is significant for China's efforts to build a marine power and achieve high-quality development of the marine economy. The existing studies have deficiencies in explaining the complex interaction among multiple land-based influence factors on MEE. Based on the Technology-Organization-Environment (TOE) framework and combined with the characteristics of government behavior under the background of the Chinese system, this study puts forward an integrated analysis framework to understand the differences in MEE among different coastal areas. Taking 11 coastal areas in China as cases, the fuzzy-set qualitative comparative analysis (fsQCA) method is used for configuration analysis, and the findings are as follows: (1) Conditions derived from technology, organization, and environment cannot, on their own, constitute the necessary conditions for high or low MEE, indicating that a single condition has limited explanatory power on MEE.(2) There are three configuration paths for high MEE: a path of development based on innovation, a path of ecological industry based on unique geographical location, and a path of development based on political pressure. Among them, the path of development based on innovation plays a more important role in improving MEE and has more value in making effective policy. (3) There are two ways to have a low MEE: one with a lot of innovation elements and another with a lot of environmental regulation and political pressure on the technology. These two paths imply that the lack of green technology capability is an important conditional variable for low MEE. The findings of this study will help people understand MEE's long-term development path in a more rational way and give them useful practical advice.
Auction-based location-aware task offloading strategy in edge cloud environment
Authors: Pei Cui , Fan Guisheng , Yu Huiqun , Yue Yiming 
Published: April 2022

Abstract
With the advent of 5G high-bandwidth and low-latency era, mobile data traffic has exploded. Most of the current researches on task offloading in edge cloud environments mainly focuses on minimizing latency, energy consumption and cost.But problems such as resource supply and demand relationship in the actual cloud market, participants’ malicious competition behavior and task mobility will affect the actual offloading results, and the security of the offloading process cannot be guaranteed. Therefore , an Auction Based Location-aware Task Offloading (ABLTO) strategy is proposed, which uses the combined double auction model to meet users’ needs for various types of resources. Then the buyer sorts according to its bid density, and the seller is ranked according to the authenticity of its bid and the distance from the buyer, and incentive/punishment measures are set to encourage the seller to deliver in good faith. The auction is deployed in the smart contract to ensure the authenticity and reliability of the process. Finally, the authenticity, fairness and economic benefits of the strategy are verified through simulation experiments.
Survival of Lactobacillus reuteri UBLRu-87 during passage through the unique in vitro gut model system
Authors: Ahire J. J. , Mokashe N. U. , Kashikar M. S. , Madempudi R. S. 
Published: April 2022

Abstract
In this study, the survival of Lactobacillus reuteri UBLRu-87 during passage through the unique in vitro gut model system was investigated. The viability of strain with and without glucose in GIT was determined by using pour plate and flow cytometer analysis. Lactic acid (D- and L-) production and residual glucose levels were measured as indicators of metabolic activity. In results, as per the plate count method, bacterial survival remained 100 % at 2 h of stomach phase (with and without glucose). Later at 3 h of intestinal phase, it reduced to 52 % with glucose and 100 % without glucose. At 24 h of colonic incubation, viability increased to 143 %, where bacteria utilized ~ 54% of glucose and produced 7.73 ± 0.70 mmol/L L-lactic acid and 0.22 ± 0.01 mmol/L D-lactic acid. In the absence of glucose, strain count was increased from zero to 4.95 ± 0.10 log10 CFU/mL at 24 h of colon incubation. In flow cytometer analysis, the survival of strain at 2 h of stomach phase was 109 % with glucose and 105 % without glucose. Later, it reduced to 74 % at 3 h of the intestinal phase (with and without glucose). At the end of the 24 h colonic incubation, the bacterial viability was increased to 111 % with glucose and 108 % without glucose. In conclusion, under harsh intestinal conditions without glucose, UBLRu-87 cells lose their ability to grow and multiply, but keep a viable but non-culturable (VBNC) state. After favorable conditions in colon, strain VBNC state resuscitate to culturable state. Besides this, glucose favors strain protection and viability enhancing effects during passage of strain throughout the GIT.
Rhein Promotes the Proliferation of Keratinocytes by Targeting Oestrogen Receptors for Skin Ulcer Treatment
Authors: Xu Ning , Chen Yuanran , Guo Dongjie , Deng Yu , Guo Wanjun , Liu Xin , Wang Yi , Lu Hanzhi , Zhu Jianyong , Li Fulun 
Published: April 2022

Abstract
Background
The Sheng-ji Hua-yu (SJHY) formula is a quite effective Traditional Chinese Medicines (TCM) in the treatment of delayed diabetic wounds. Previous research has shown that the SJHY formula has significant anti-inflammatory and wound-healing effects, but the precise mechanism remains unknown. The purpose of this study was to evaluate the effects of rhein, a compound extracted from SJHY formula, in keratinocytes and to investigate the underlying mechanisms.
Methods
Microscale thermophoresis (MST) technology was used to confirm that rhein binds directly to oestrogen receptors (ERs). Rhein was then used to treat keratinocytes in vitro. Cell cycle and proliferation analysis, Real-time polymerase chain reaction (RT-PCR) and Western-blot were conducted.
Results
Rhein increased the proportion of cells in the S phase of the cell cycle and promoted keratinocyte proliferation. ICI 182780, an ER inhibitor, was also used to treat keratinocytes. The expression of c-myc mRNA and protein induced by rhein was antagonized by ICI 182780, indicating that this induction is ER dependent. Intervention with ICI 182780 had no effect on the upregulation of FosB and JunD, indicating that activator protein 1 (AP-1) members (FosB and JunD) are involved in rhein-induced c-myc mRNA and protein expression but does not require the ER.
Conclusion
The present study found that rhein stimulates keratinocyte proliferation by activating the oestrogen signalling pathway via the oestrogen receptor, which induces the expression of c-myc in collaboration with FosB and JunD, thereby accelerating the process of re-epithelialization.
Use of ketamine in patients with refractory severe asthma exacerbations: systematic review of prospective studies.
Authors: Via Luigi La , Sanfilippo Filippo , Cuttone Giuseppe , Dezio Veronica , Falcone Monica , Brancati Serena , Crimi Claudia , Astuto Marinella 
Published: April 2022

Abstract
Background: Asthma is a heterogeneous disease with wide range of symptoms. Severe asthma exacerbations (SAEs) are characterized by worsening symptoms and bronchospasm requiring emergency department visits. In addition to conventional strategies for SAEs (inhaled β-agonists, anticholinergics, and systemic corticosteroids), another pharmacological option is represented by ketamine. Objective: To explore the role of ketamine in refractory SAEs.Methods: We performed a systematic search on PubMed and EMBASE up to August 12th, 2021. We selected prospective studies only, and outcomes of interest were: oxygenation/respiratory parameters, clinical status, need for invasive ventilation and effects on weaning.Results: We included a total of seven studies, five being randomized controlled trials (RCTs, population range 44-92 patients). The two small prospective studies (n=10 and n=11) did not have a control group. Four studies focused on adults, and three enrolled a pediatric population. We found large heterogeneity regarding sample size, age and gender distribution, inclusion criteria (different severity scores, if any) and ketamine dosing (bolus and/or continuous infusion). Of the five RCTs, three compared ketamine to placebo, while one used fentanyl and the other aminophylline. The outcomes evaluated by the included studies were highly variable. Despite paucity of data and large heterogeneity, an overview of the included studies suggests absence of clear benefit produced by ketamine in patients with refractory SAE, and some signals towards side effects. Conclusions: Our systematic review does not support the use of ketamine in refractory SAE. A limited number of prospective studies with large heterogeneity was found. Well-designed multicenter RCT are desirable.
Comparison of the Effects of Digoxin and Bevacizumab on Oxygen-Induced Retinopathy Rat Model
Authors: Balbaba Mehmet , Güler Seda Duran , Ulaş Fatih , Yıldırım Hakan , Canleblebici Mehmet , İncili Canan Akdeniz 
Published: April 2022

Abstract
Purpose: To evaluate the effect of digoxin on oxygen-induced retinopathy (OIR) model and compare the results with bevacizumab treatment.Methods: Twenty-eight newborn Sprague-Dawley rats were randomly divided into four groups:control group, normoxia+intraperitoneal (ip) normal saline (NS); OIR group, OIR+ip NS; OIR+digoxin group, OIR+ip 0.1 mg/kg of digoxin; and OIR+bevacizumab group, OIR+ip 2.5 mg/kg of bevacizumab. The rats were exposed to 50% oxygen for 24 h, followed by 10% oxygen for 24 h, to induce OIR. This cycle was repeated seven times until 14 days postnatal (P). In all groups, single-dose injections were administered on P15th day. Histopathological and immunohistochemical examinations were performed at the end of the study. Results: The mean neovascular cell nuclei counts (NVCN) of the four groups were 9.00±3.16, 41.80±11.44, 19.38±2.20, and 16.00±2.62. The mean NVCN count was significantly reduced in the treatment groups compared to the OIR group (p<0.001). The mean NVCN count was similar between the treatment groups (p=0.078). In immunohistochemical staining, the immunoreactivity values of the vascular endothelial growth factor (VEGF) were 0.01±0.00, 1.65±0.30, 0.09±0.08, and 0.04±0.02, and the tumor necrosis factor alpha (TNF-α) values were 0.10±0.00, 1.12±0.18, 0.18±0.13, and 0.14±0.05. In the OIR group, VEGF and TNF-α immunoreactivity increased markedly compared to the control group (p<0.001). VEGF and TNF-α immunoreactivity of the treatment groups decreased significantly compared to the OIR group (p<0.001). VEGF and TNF-α immunoreactivity were similar between the treatment groups (VEGF:p=0.752; TNF-α:p=0.099).Conclusions: Retinal neovascularization was significantly suppressed by digoxin treatment, and this effect was comparable to bevacizumab in the OIR model.
New insights to estimate reproduction numbers along an epidemic
Authors: Sebastiani Giovanni , Spassiani Ilaria 
Published: April 2022

Abstract
In this paper, we focus on the problem of estimating the reproduction number Rt along an epidemic, as it represents the most used tool to study and control this phenomenon. In particular, we focus on two issues. First, when dealing with the standard estimator for Rt , we consider the use of positive test data as an alternative to the first symptoms ones, that are typically used. We study both theoretically and empirically the relationship between the two Rt sequences obtained. Second, we describe a new estimator for Rt that is not affected by the drawbacks due to the hypothesis of its local constancy assumed in the standard approach. We illustrate the results obtained by applying the proposed methodologies to both real SARS-CoV-2 Italian data, and two types of simulated ones. In both the two cases, we apply specific methods we have developed to reduce both sistematic and random errors affecting the data. Our results show that the Rt along an epidemic can be estimated with some advantages by using the positive test sequence, and that the estimator we propose outperforms the standard one. We hope that these new methodologies could help to study and control epidemics, and in particular the current SARS-CoV-2 pandemic.
Sulfate Erosion Investigation on FRP-confined Concrete in Cold Region
Authors: ji yongcheng , zou yunfei , li wei 
Published: April 2022

Abstract
Fiber-reinforced polymer (FRP) confined concrete is regarded as an innovative and economical approach for structural repairation. Two typical materials (carbon fiber reinforced polymer (CFRP) and glass fiber reinforced polymer (GFRP)) are selected in this study to investigate the concrete strengthen effect in a severe environment. The resist ability of FRP-confined concrete is discussed when subjected to coupled erosion between sulfate erosion and freeze-thaw cycles. Electron microscopy examines concrete's surface and interior degradation during coupled erosion. The corrosion degree and principle of sodium sulfate are analyzed using pH, SEM electron microscope, and EDS energy spectrum. The axial compressive strength test is used to evaluate the reinforcement of the FRP-confined concrete column, and the stress-strain relationship for various FRP-confined techniques in a coupled erosion environment is obtained. The error analysis is performed to calibrate the experimental test result using four existed prediction models. All observations indicate that the deterioration process of FRP-confined concrete is complicated and dynamic under coupled effect. Sodium sulfate initially increases the initial strength of concrete. However, subsequent freeze-thaw cycles may aggravate concrete fractures, while sodium sulfate further degrades the strength of concrete through the cracking development. A precise numerical model is presented to simulate the stress-strain relationship, which is critical for the design and life cycle assessment of FRP-confined concrete.
Epigenetic genes alterations in metastatic solid tumors: results from the prospective precision medicine MOSCATO and MATCH-R trials
Authors: Postel-Vinay Sophie , Martin-Romano Patricia , Colmet-Daage Leo , Morel Daphne , Baldini Capucine , Verlingue Loic , Bahleda Rastiav , Gazzah Anas , Champiat Stephane , Varga Andreea , Michot Jean Marie , Ngo-Camus Maud , Nicotra Claudio , Marabelle Aurelien , Soria Jean Charles , Rouleau Etienne , Lacroix Ludovic , Hollebecque Antoine , Massard Christophe 
Published: April 2022

Abstract
Purpose: Although the role of epigenetic alterations in oncogenesis has been well studied, their prevalence in metastatic solid tumors is still poorly described. We therefore aimed at: (i) describing the presence of epigenetic gene alterations (EGA) - defined by an alteration in a gene encoding an epigenetic regulator; and (ii) evaluating their relationship with clinical characteristics and outcome in patients (pts) included in prospective molecular profiling trials.Patients and methods: On-purpose tumor biopsies from pts with metastatic solid tumors enrolled in the Gustave Roussy-sponsored MOSCATO (NCT01566019) and MATCHR (NCT02517892) trials were molecularly profiled using Whole Exome Sequencing (WES). Alterations in 176 epigenetic genes were assessed and classified as pathogenic variants (PV) or non-pathogenic variants by a molecular tumor board. Clinical characteristics and outcome were collected.Results: Between Dec 2011 and Oct 2016, WES was successfully performed in 292 pts presenting various solid tumors. We found 496 epigenetic gene alterations in 134 patients (49%), including 237 pathogenic variants in 86 patients; 63 tumor samples (47%) presented ≥3 EGAs. The median number of previous treatment lines was 3 (1 – 10). The most frequently altered genes were KMT2D and KMT2C (16% each), ARID1A and SETD2 (10% each) and KMT2A (8%).; 31% of EGA co-occurred with a driver gene alteration (p < 0.001). Outcome was not correlated with the presence of EGA.Conclusions: Epigenetic alterations occur frequently in metastatic solid tumors. With the current development of epigenetic modifiers, they increasingly represent actionable targets. Such genes should now be systematically analyzed in molecular profiling studies.
LC-MS proteomic profiling of trace FFPE samples in gastrointestinal cancer
Authors: Guo Chunmei , Li Lingling , Liu Hui , Ding Chen 
Published: April 2022

Abstract
Background: Early screening and diagnosis of gastrointestinal cancer would be benefit for patients’ prognosis. However, the complexity of cancer progression and the extreme trace amount of tissue samples in different stages have limited in portraying the characterization of early-stage cancer. Here, We disclosed the biological pathways and built an integrated proteomic map of trace FFPE samples in gastrointestinal cancer.Methods: In this study, a quantitative proteomic method with chromatography with mass spectrometry (LC-MS/MS) was used to analyse the different amounts of peptides between the trace duodenum and gastric cancers. Results: We performed a comprehensive proteomic landscape of trace formalin-fixed, paraffin-embedded (FFPE) samples with 5 different amounts of peptides (AOPs) in 40 gastrointestinal cancer samples. Proteomic analysis disclosed the different features of high/low proteins in duodenum and gastric cancers, and revealed the gradually decreased proteins number with the dilutions of AOP. In addition, we found the high coverage of identified proteins when the input of AOPs were no less than 0.5 µg. Proteomic methods represented the loss of tumor heterogeneity when the input of AOPs were no more than 0.5 µg. We also illustrated the dominant pathways in duodenum cancer and gastric cancer, and found the loss function when the input of AOPs was no more than 0.5 µg. Collectively, our study built an integrated proteomic map of trace FFPE samples in gastrointestinal cancer, and provided a valuable resource for clinical researches, especially for early-stage cancer.Conclusion: We disclosed the biological pathways of the trace samples, and revealed the high coverage (> 60%) of identified proteins when the input of AOPs was no less than 0.5 µg, keeping the primary functions of tissues. These findings provided a new insight in early-stage cancer in the clinic.
An Efficient Framework for Video Documentation of Bladder Lesions for Cystoscopy: A Proof-of-Concept Study
Authors: Eminaga Okyaz , Ge T. Jessie , Shkolyar Eugene , Laurie Mark A. , Lee Timothy J. , Hockman Lukas , Jia Xiao , Xing Lei , Liao Joseph C. 
Published: April 2022

Abstract
The identification of relevant frame sequences in an endoscopy video is labor-intensive and complicated by the poor data integrity of endoscopic information. We designed a surgeon-guided framework to efficiently manage the visual data from commonly performed bladder endoscopy (cystoscopy) videos. Screenshots of bladder lesions were captured during cystoscopy during transurethral resection of bladder tumor, then manually labeled according to case identification, date, lesion location, imaging modality, and pathology. The framework used the screenshot to search for and extract a corresponding 10-second video clip. Each video clip included a one-second space holder with a QR barcode informing the video content. The success of the framework was measured by the secondary use of these short clips and the reduction of storage volume required for video materials. From 86 cases, the framework successfully generated 249 video clips from 230 screenshots, with 14 erroneous video clips from 8 screenshots excluded. The HIPPA-compliant barcodes provided information of video contents with a 100% data completeness. A web-based educational gallery was curated with various diagnostic categories and annotated frame sequences. Compared with the unedited videos, the informative short video clips reduced the storage volume by 99.5%. In conclusion, our framework expedites the generation of visual contents with surgeon’s instruction for cystoscopy and potential incorporation of video data towards applications including clinical documentation, education, and research.
Light Absorptance of Algal Films for Photosynthetic Rate Determinations
Authors: RITCHIE Raymond J. , SMA-AIR Suhailar , RUNCIE John W. 
Published: April 2022

Abstract
A Reflectance Absorptance Transmittance (RAT) meter, developed for routine measurements of Abtλ nm on leaves, was used to measure absorptance of algal films mounted on glass fibre filter disks for use in PAM (Pulse Amplitude Modulation) fluorometry. Absorptance readings (Abtλ nm) are essential to quantitate the photosynthetic electron transport rate from PAM data and are also helpful if algal disks are used in an IRGA (InfraRed Gas Analyzer). Measurements were made on Synechococcus R-2 (Cyanophyceae), Acaryochloris marina (Chlorobacteria), Chlorella vulgaris (Chlorophyceae), Chaetoceros sp. (Bacillariophyceae) and Isochrysis sp. (Haptophyta) in blue (445 nm), green (525 nm) and red (625 nm) light. Plots of Abtλ vs. Chl a (mg Chl a m− 2) or Chl d for Acaryochloris (mg Chl d m− 2) of the algae followed exponential saturation curves (Abt%λ = Abt%λ, ∞ (1-e− k×Chl)) usually reaching asymptotes in the blue light used by blue-diode PAM machines. Synechococcus, Chlorella, Chaetoceros and Isochrysis Abt%445nm saturates ≈ 50–100 mg Chl a m− 2 (Synechococcus: Abt%445 nm, max = 77.3 ± 0.94, ± 95% conf. limits, Chlorella: Abt%445 nm, max = 77.0 ± 2.06, Chaetoceros: Abt%445 nm, max = 80.6 ± 1.87; Isochrysis: 83.2 ± 8.06 mg Chl a m− 2); Acaryochloris above about 100 mg Chl d m− 2. Asymptotic loading of cells was in general easily achieved in blue light in the case of Synechococcus, Chlorella and Acaryochloris but not in green, red or “white” light. It was difficult to achieve asymptotic loading for Chaetoceros and Isochrysis due to mucilaginous blockage of the filter disks.