bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self-Care Practice and Associated Factors Among Patients with Type 2 Diabetes Mellitus at a Referral Hospital in Northern Ethiopia – a Mixed Methods Study
Authors: Zewdie Segenet , Moges Getachew , Andargie Assefa , Habte Bruck Messele 
Published: March 2022

Abstract
Introduction: Diabetes self-care is important to maintain optimal glycemic control and prevent debilitating and costly complications. Diabetes self-care may be improved through the identification of individual and group barriers to regimen adherence and subsequent development of specific plans to overcome these barriers. This study assessed the self-care practice and associated factors among type 2 diabetes patients attending their treatment at Dessie Referral Hospital, Dessie, North-Eastern Ethiopia. Methods: The study employed parallel mixed methods design which followed a cross-sectional interview and in-depth interview methods respectively from September to October 2019. The collected data were subjected to descriptive and inferential analysis for the quantitative part and a thematic analysis for the qualitative part. The protocol for this study was ethically approved (CMHS/013/519/12). Results: A total of 328 type 2 diabetes patients participated in the study of which 50.3% reported good self-care practice. Being in the age category of 60–69 years old (AOR = 0.334, 95% CI (0.135, 0.951)), being ≥ 70 years old (AOR = 0.359, 95% CI (0.135,0.951)), having complications (AOR = 1.956, 95% CI (1.172, 3.262)), having co-morbidity (AOR = 0.443, 95%CI (0.262, 0.749)) and diabetes education (AOR = 2.684, 95%CI (1.633,4.412) were significantly associated with good diabetes self-care. Accessibility, social support, knowledge and beliefs and diabetes related morbidities were identified as barriers to diabetes self-care by the patients. Conclusion: The findings from this study revealed that only half of the type 2 diabetes patients who participated in this study reported good self-care practice. Different factors including diabetes education were significantly associated with good diabetes self-care according to the quantitative study. This was supported by the findings from the qualitative part and thus the recommendation to strengthen diabetes health education to patients and their families.
Different Responses of Banana Classical AGP Genes and Cell Wall AGP Components to Low-temperature between Chilling Sensitive and Tolerant Cultivars
Authors: Liu Jing , Meng Jian , Chen Houbin , Li Xiaoquan , Su Zuxiang , Chen Chengjie , Ning Tong , He Zhenting , Dai Longyu , Xu Chunxiang 
Published: March 2022

Abstract
Classical arabinogalactan proteins (AGPs) belong to glycosylphosphatidylinositol-anchored proteins, which are proved to be involved in signaling and cell wall metabolism upon stresses. However, rare information is available on the roles of classical AGPs in low temperature (LT) tolerance. Cultivation of banana in tropical and subtropical region is seriously threatened by LT stress. In the present study, 17 classical MaAGPs and nine MbAGPs in banana A and B genome were identified and characterized, respectively. Great diversity was present among different classical MaAGP/MbAGP members while five members (AGP3/6/11/13/14) showed 100% identity between these two gene families. We further investigated different responses of classical AGPs to LT between a chilling sensitive (CS) and tolerant (CT) banana cultivars. In addition, different changes in the temporal and spatial distribution of cell wall AGP components under LTs between these two cultivars were compared using immunofluorescence labeling. Seven classical MbAGPs were upregulated by LT(s) in the CT cultivar. Classical MaAGP4/6 was induced by LT(s) in both cultivars while MaAGP1/2/9/16/17 only in the CT cultivar. Moreover, these genes showed significantly higher transcription abundance in the CT cultivar than the CS one under LT(s) except classical MaAGP4. Similar results were observed with the epitopes of JIM13 and LM2 antibodies. The antigens of these antibodies and classical MaAGP1/2/6/9/16/17 might be related to LT tolerance of banana. These results provide additional information about plant classical AGPs and their involvement in LT tolerance, as well as their potential as candidate genes to be targeted when breeding CT banana.
miR-517b-3p promotes the progression of portal vein tumor thrombus via activating Wnt/β-catenin signaling pathway in Hepatocellular carcinoma.
Authors: Ke Rui-Sheng , Huang Kun-Zhai , Bao De-sheng , Yang Jing-Rui , Wang Hua-xiang , Lv Li-Zhi , Jiang Yi , Liu Zhao-Hui , Zhang Fu-Xing 
Published: March 2022

Abstract
Aims: The aim of this study was to investigate the expression patterns and prognostic value of microRNA-517b-3p (miR-517b-3p) in hepatocellular carcinoma (HCC) patients with portal vein tumor thrombus (PVTT).Methods: Expression of miR-517b-3p in PVTT tissues and cells was estimated using qRT-PCR. The significance of miR-517b-3p were explored by Kaplan-Meier survival analysis, Cox regression assay and ROC analysis. Cell experiments were performed to examine the functional role of miR-517b-3p during progression of PVTT. The Biological process and Biological pathway analysis analyses were conducted through GSEA and FunRich. The protein-protein interaction (PPI) network of the DEGs was established through cBioPortal website. Results: miR-517b-3p upregulated in both PVTT tissues and cells compared with the controls. The upregulated miR-517b-3p served as a potential diagnostic biomarker to distinguish PVTT from PT and controls, associated with poor overall survival and acted as an independent prognostic factor. The cell proliferation, migration and invasion were proved to be enhanced by overexpression of miR-517b-3p. Wnt/β-catenin signaling was suppressed by miR-517b-3p knockdown and might be involved in progression of PVTT. Conclusion: miR-517b-3p may promote PVTT cell proliferation, migration and invasion via activating Wnt/β-catenin signaling pathway. miR-517b-3p overexpression in PVTT samples, and serves as a candidate diagnostic and prognostic biomarker in HCC patients with PVTT.
Alkaline Phosphatase to Albumin Ratio: A Novel Prognostic Biomarker for Hepatocellular Carcinoma after Microwave Ablation as the Initial Treatment
Authors: Chen Bin , An Tailai , Li Xinming , Sun Xinfeng , Sheng Cuiyun , Wang Yan , Xie Zonggui , Quan Xianyue 
Published: March 2022

Abstract
Background
As a new serum-based biomarker, albumin to alkaline phosphatase ratio (AAPR) has been studied among several cancers. However, alkaline phosphatase to albumin ratio (APAR) has not been investigated among hepatocellular carcinoma (HCC) patients undergoing microwave ablation (MWA) as the initial therapy.
Methods
228 HCC patients undergoing MWA as the initial treatment were included in this study. Firstly, receiver operating curve (ROC) analysis was performed to identify the optimal cutoff values for APAR and other biomarkers. Then, the associations between APAR and clinicopathological variables were assessed. Additionally, Kaplan-Meier analysis was accomplished to evaluate the impacts of APAR on prognosis of HCC patients. Ultimately, Cox regression analysis was performed to identify independent prognostic factors for HCC patients.
Results
It was initially revealed that the optimal cutoff value for APAR was 2.44 (P < 0.001). Then it was demonstrated that high APAR was significantly associated with tumor size (P = 0.043). Subsequently, through Kaplan-Meier analysis, it was revealed that high APAR was significantly associated with worse overall survival (OS, P < 0.001) and disease-free survival (DFS, P < 0.001). Ultimately, along with tumor size (P = 0.018, HR = 2.292, 95%CI: 1.152–4.561), high APAR (P < 0.001, HR = 5.881, 95%CI: 3.093–11.185) was demonstrated to be an independent predictive factor for overall survival (OS). Similarly, along with Child-Pugh grade (P = 0.001, HR = 3.467, 95%CI: 1.705–7.050), GAR (P = 0.004, HR = 2.152, 95%CI: 1.281–3.613), and number of tumors (P = 0.015, HR = 2.678, 95%CI: 1.213–5.917), high APAR (P = 0.032, HR = 1.572, 95%CI: 1.040–2.377) was demonstrated to be an independent predictive factor for disease-free survival (DFS).
Conclusion
APAR was a reliable biomarker predicting survival of HCC patients.
Gypenosides inhibits the growth and induces apoptosis of glioma cells by promoting autophagy
Authors: Liu HaiYun , Ji YuLong , Lv QiaoLi , Chen JunJun , Du Hong , Wu JinTao , Lin QianXia , Fang YongQing , Wang LiChong , Yu Kai , Lu TianZhu , Zhang Zhe , Tan ZiLong , Wang DaPeng , Chen ShuHui 
Published: March 2022

Abstract
Gypenosides (Gyp), a Chinese medicine and an effective ingredient extracted from Gynostemma pentaphyllum, plays vital roles in the progression of various cancers. However, its biological functions and mechanisms in glioma remain unclear. We evaluated the anti-cancer mechanism of Gyp in glioma to determine its potential therapeutic value. In human glioma cells, we evaluated cell viability using MTS assays and cell mobility using Transwell invasion and scratch migration assays. Additionally, we assessed apoptosis using flow cytometry and Hoechst 33342 staining, and autophagy flow was detected by Fluorescence microscope. The levels of migration, apoptosis and autophagy-related protein were examinated by Western blot. Gyp inhibited the proliferation, migration and invasion of glioma cells by down-regulating the level of matrix metalloproteinase (MMP)-2 and MMP-9. Moreover, Gyp promoted apoptosis of glioma cells by inducing Bax and cleaved-caspase-9 activation and down-regulating Bcl-2. Notably, autophagy was activated by treating with Gyp in U251 and U87 cells through down-regulation of p62 protein and up-regulation of Beclin1 and LC3 II proteins. Interestingly, treatment with autophagy flux inhibitor Baf-A1 or 3-MA blocked the inhibitory effects of Gyp in glioma cells. Combined application of Gyp with Baf-A1 or 3-MA slightly increased cell death, suggesting that induction of autophagy impaired enzyme accumulation and contributed to the growth inhibition of glioma cells. In summary, Gyp suppressed the proliferation, migration and invasion and induced apoptosis of glioma cells by promoting autophagy, suggested the potential value of Gyp in the combined treatment of glioma.
Reduced performance of community bednets against pyrethroid-resistant Anopheles funestus and Anopheles gambiae, major malaria vectors in Cameroon
Authors: Yipmo Emilie S. L. , Tchouakui Magellan , Menze Benjamin D. , Mugenzi Leon M.J , Njiokou Flobert , Wondji Charles S. 
Published: March 2022

Abstract
Background: Long-lasting insecticidal nets are a vital tool in the fight against malaria vectors. However, their efficacy in the field can be impacted by several factors including patterns of usage, net age, resistance, and the delayed mortality effect that could influence malaria transmission. Here, we investigated the effectiveness of various brands of LLINs from markets and households in Cameroon, on pyrethroid-resistant mosquitoes and assessed their post-exposure effect.Methods: After quality control on susceptible lab strain, immediate and delayed mortality effects of LLINs (bought and collected from households in Elende in 2019) exposure were evaluated using standard WHO cone tests on Anopheles gambiae and Anopheles funestus populations collected from the Centre region of Cameroon. Alive female mosquitoes were genotyped for various resistance markers at different time-points post-exposure to evaluate the impact of insecticide resistance on the efficacy of bednets.Results and conclusion: High mortality rates were obtained with the laboratory susceptible strain when exposed to all pyrethroid-only brands of purchased nets (Olyset®Net, Super Net, PermaNet®2, Yorkool®, Royal Sentry®) (68.66 ± 8.35% to 93.33 ± 2.90%). However, low mortality was observed against wild An. funestus mosquitoes (0±0% to 28±6.7%) indicating a reduced performance of these nets against field mosquitoes. In addition, bednets collected from households showed reduced efficacy on the laboratory strain (19%- 66% mortality) and displayed a significant loss of efficacy against the local wild strains (mortality 0 ± 0% to 4 ± 2.6% for An. gambiae s.l. and 0 ± 0% to 8 ± 3.2% for An. funestus). However, compared to the unexposed group, mosquitoes exposed to bed nets showed a significantly reduced longevity indicating that the efficacy of these nets is not completely lost. CYP6P9a-RR and L119F-GSTe2 resistant mosquitoes had greater longevity after exposure to the Olyset net compared to their susceptible counterparts pointing the impact of resistance on bed nets efficacy and delay mortality. These findings present evidence of the actual impact of resistance on the quality and efficacy of LLINs in use in the community but also on the level of resistance of local vector populations to these nets highlighting the need to use new generation nets not relying only on pyrethroids.
Land use pattern optimization method in national territory spatial planning: A case study of Changzhi City, Shanxi Province
Authors: Wang Juan , Lv jiaqi , Zhang Wenping , Chen Tianqian , Yang Yang , Wu Jinjin 
Published: March 2022

Abstract
Land use pattern has always been affected by urban development, and its optimization is of great significance to high-quality urban development, but the land use structure in coal cities has been rarely explored. Changzhi City, as a central city in the southeast region of Shanxi Province, is a large coal province with a superior ecological environment. Under the background of land spatial planning and ecological civilization construction, the land use structure and ecological problems of this city have attracted great attention. This research aims to explore the land use structure optimization method and provide new ideas for the national territory spatial planning of Changzhi City. On the basis of the 2010–2018 land use data of Changzhi City, the current problems in land use are analyzed, the construction land intensiveness, the potential of cultivated land reserve resources, and the potential of rural construction land consolidation are evaluated, and GeoSOS-FLUS is used to justify the conversion of various land types, providing the focus and direction of land use structure optimization and layout adjustment. Results show that the spatial distribution of land in Changzhi City presents the “forest-farm-forest” characteristic, the construction land intensive utilization of Changzhi City is low, and the potential of rural construction land consolidation is high. In addition, the conversion probability of cultivated land in Tunliu district and the conversion probability of construction land close to Luzhou district are relatively higher, which can be used as key areas for the future development of Changzhi City. In the process of urban development, cultivated land protection and construction land demand should be balanced.
Observation and identification of autofluorescent urine crystals may be linked to a sign of urolithiasis
Authors: Chiang Huihua Kenny , Lin Syue-Liang , Chung Chen-Yuan , Chen Zih-Ting , Huang Chih-Chia , Li Yun-Zhen , Huang Eric Yi-Hsiu 
Published: March 2022

Abstract
Urolithiasis is a common disease of the urinary system. Its recurrence rate is high and may increase medical expenses. Urine stones are composed of urine crystals and other impurities. We discovered the existence of autofluorescence in some of the urine crystals, especially in urolithiasis patients. The fluorescent molecule existed in urine crystals was verified and identified. We have applied micro-Raman and fluorescence microscopy to classify the urine crystals, used confocal laser scanning microscopy (CLSM) to examine the 3D images and spectra of autofluorescence in crystals, used Fourier transform infrared spectroscopy (FTIR) and mass spectrometry (MS) to identify the type of fluorophore in the autofluorescent urine crystals in urine. Riboflavin was identified as one of the major fluorophores in these autofluorescent urine crystals. The prevalence rates of the autofluorescent crystals in urolithiasis patients and subjects without the history of urolithiasis were to gather statistics. We observed that 80% of urolithiasis patients had autofluorescent crystals. Contrastingly, such crystals existed in only 7% of subjects without the history of urolithiasis. The presence of autofluorescent urine crystals may be linked to a sign of urolithiasis.
Identification of hub genes in the subacute spinal cord injury in rats
Authors: Yan Lei , Fu Jiawei , Dong Xiong , Chen Baishen , Hong Hongxiang , Cui Zhiming 
Published: March 2022

Abstract
Background: Spinal cord injury (SCI) is a common trauma in clinical practices. Subacute SCI is mainly characterized by neuronal apoptosis, axonal demyelination, Wallerian degeneration, axonal remodeling, and glial scar formation. It has been discovered in recent years that inflammatory responses are particularly important in subacute SCI. However, the mechanisms mediating inflammation are not completely clear.Methods: The gene expression profiles of GSE20907, GSE45006, and GSE45550 were downloaded from the GEO database. The differentially expressed genes (DEGs) and weighted correlation network analysis (WGCNA) were performed using R software, and functional enrichment analysis and protein-protein interaction (PPI) network were performed using Metascape. Module analysis was performed using Cytoscape. Finally, the relative mRNA expression level of central genes was verified by RT-PCR.Results: A total of 206 candidate genes were identified, including 164 up-regulated genes and 42 down-regulated genes. The PPI network was evaluated, and the candidate genes enrichment results were mainly related to the production of tumor necrosis factors and innate immune regulatory response. Twelve core genes were identified, including 10 up-regulated genes and 2 down-regulated genes. Finally, seven hub genes with statistical significance in both the RT-PCR results and expression matrix were identified, namely Itgb1, Ptprc, Cd63, Lgals3, Vav1, Shc1, and Casp4. They are all related to the activation process of microglia.Conclusion: In this study, we identified the hub genes and signaling pathways involved in subacute SCI using bioinformatics methods, which may provide a molecular basis for the future treatment of SCI.
Clinical evaluation of subcutaneous emphysema occurred in dental treatments
Authors: Saito Yuki , Kawai Tadashi , Kogi Shintaro , Onodera Kei , Obara Mizuki , Takeda Kei , Komatsu Yuko , Chiba Toshimi , Yamada Hiroyuki 
Published: March 2022

Abstract
Background
Subcutaneous emphysema is an uncommon incident in dental treatments. This study was designed to investigate the subcutaneous emphysema cases occurred in dental treatments.
Methods
Eleven patients were diagnosed as a subcutaneous emphysema in our hospital from 2011 to 2021. These patients were evaluated for age, sex, medical history, origin tooth, dental procedure, cause, range, healing period, duration of antibiotic, and complication. And it was analyzed statistically whether the age, range, or dental procedure had influences for healing period.
Results
The average age was 48.0 ± 20.0 years. The incidence of subcutaneous emphysema was not related to age but was more common in females. Of the 11 patients, 4 had subcutaneous emphysema that had spread to the chest. It seemed that surgical procedure using an air turbine, or a handpiece is at greatest risk of subcutaneous emphysema. The average healing period, and duration of antibiotics was 8.18 ± 1.78 days, and 10.09 ± 4.30 days, respectively. The statistical results showed a significant difference in the healing period between presence and absence of some medical history.
Conclusions
This study suggests that subcutaneous emphysema is more frequent in surgical procedures using an air turbine or a handpiece, and that the presence or absence of some diseases affects the healing period.
PEMBUATAN PROFIL DESA GERBOSARI
Authors: Devi Laksmi Yustika , Hidayati Wahyu 
Published: March 2022

Profil desa dan kelurahan diperlukan untuk mengetahui gambaran potensi dan tingkat perkembangan desa dan kelurahan yang akurat, komprehensif dan integral seperti dinyatakan dalam Permendagri Nomor 12 Tahun 2007. Profil desa dan kelurahan merupakan gambaran menyeluruh tentang karakter desa dan kelurahan yang meliputi data dasar keluarga, potensi sumber daya alam, sumber daya manusia, kelembagaan, prasarana dan sarana serta perkembangan kemajuan dan permasalahan yang dihadapi desa dan kelurahan. Profil desa dan kelurahan harus dapat berfungsi sebagai sumber informasi potensi desa dan kelurahan. Oleh karena itu, data profil haruslah tersedia, lengkap dan akuntabel. Melalui kegiatan Pengabdian Kepada Masyarakat Sekolah Vokasi Tahun 2019, Departemen Ekonomika dan Bisnis SV UGM berusaha membantu Desa Gerbosari yang terletak di Kecamatan Samigaluh, Kabupaten Kulon Progo dalam upaya pembuatan profil desa. Berdasarkan identifikasi permasalahan, profil Desa Gerbosari terbaru telah memuat beberapa data dan informasi seperti yang dipersyaratkan dalam Permendagri Nomor 12 Tahun 2007, terutama untuk data potensi sumber daya alam. Namun, data yang lain seperti data dasar keluarga, sumber daya manusia, kelembagaan, prasarana dan sarana serta perkembangan kemajuan dan permasalahan yang dihadapi desa dan kelurahan masih belum lengkap. Oleh karena itu, tujuan dari kegiatan pengabdian ini adalah: 1) melakukan pembuatan profil Desa Gerbosari yang memuat data potensi desa secara lengkap dan 2) menyajikan data profil desa yang dapat diakses baik dalam versi cetak maupun elektronik. Luaran dari kegiatan pengabdian ini adalah dokumen profil desa dan video profil desa. Evaluasi dari kegiatan ini dilakukan secara langsung pada saat kegiatan pembuatan profil. Pembuatan profil desa dilakukan dengan feedback secara kontinu oleh Perangkat Desa Gerbosari. Kata Kunci: profil desa, kelengkapan data, potensi, perencanaan, pembangunan
Key Fail Factors as Innovation Strategies to be avoided: a comparative study between Mexican and American Social Impact Startup under COVID-19 pandemic times
Authors: Mejía-Trejo Juan , Gutiérrez José Sánchez , Patiño-Karam Juan Pablo 
Published: March 2022

Purpose: This study determines the key success and key fail factors (KSF-KFF) between Mexican and American social impact startups (SIS) in COVID-19 pandemic times, as the source of innovation strategies.
Methodology. It is based on an academic-empirical KSF-SIS framework questionnaire. The survey data was on 100 Mexican/300 American CEOs-SIS in Jan-Jun-2021. Covariance-Based Structural Equation Modeling (CB-SEM) determined the model's reliability/validity to confirm the KSF, and Fuzzy set Qualitative Comparative (fsQCA) to get the KFF.
Results. Five main factors that provide to the Mexican/American SIS innovation strategies for both KSF to be developed, and KFF to be avoided.
Originality. CB-SEM confirms the KSF to achieve the opposite condition, the KFF through fsQCA for Mexican/American SIS.