bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the genetic diversity of H5 avian influenza in the UK 2020-2022
Authors: Byrne Alexander MP , James Joe , Mollett Benjamin C , Meyer Stephanie M , Lewis Thomas , Czepiel Magdalena , Seekings Amanda H , Mahmood Sahar , Thomas Saumya S , Ross Craig S , Byrne Dominic JF , McMenamy Michael J , Bailie Valerie , Lemon Ken , Hansen Rowena DE , Falchieri Marco , Lewis Nicola S , Reid Scott M , Brown Ian H , Banyard Ashley C 
Published: December 2022

AbstractSince 2020, the UK and Europe, have experienced annual epizootics of high pathogenicity avian influenza virus (HPAIV). The first during autumn/winter 2020/21 involved the detected with six H5Nx subtypes although H5N8 HPAIV dominated in the UK. Whilst genetic assessment of the H5N8 HPAIVs within the UK demonstrated relative homogeneity, there was a background of other genotypes circulating at a lower degree with different neuraminidase and internal genes. Following a small number of summer detections of H5N1 in wild birds over the summer of 2021, autumn/winter 2021/22 saw another European H5 HPAIV epizootic, that has dwarfed the prior epizootic. This second epizootic was dominated almost exclusively by H5N1 HPAIV, although six distinct genotypes were defined. We have used genetic analysis to evaluate the emergence of different genotypes and proposed reassortment events that have been observed. The existing data suggests that the H5N1 circulating in Europe during late 2020, continued to circulate in wild birds throughout 2021, with minimal adaptation, but has then gone on to reassort with AIVs in the wild bird population. We have undertaken an in-depth genetic assessment of H5 HPAIVs detected in the UK, over the last two winter seasons and demonstrate the utility of in-depth genetic analyses in defining the diversity of H5 HPAIVs circulating in avian species, the potential for zoonotic risk and whether incidents of lateral spread can be defined over independent incursion of infection from wild birds. Key supporting data for mitigation activities.ImportanceHigh pathogenicity avian influenza virus (HPAIV) outbreaks devastate avian species across all sectors having both economic and ecological impacts through mortalities in poultry and wild birds, respectively. These viruses can also represent a significant zoonotic risk. Since 2020, the UK has experienced two successive outbreaks of H5 HPAIV. Whilst H5N8 HPAIV was predominant during the 2020/21 outbreak, other H5 subtypes were also detected. The following year there was a shift in subtype dominance to H5N1 HPAIV, but multiple H5N1 genotypes were detected. Through thorough utilisation of whole-genome sequencing, it was possible to track and characterise the genetic evolution of these H5 HPAIVs in UK poultry and wild birds. This has enabled us to assess the risk posed by these viruses at the poultry:wild bird and the avian:human interface and to investigate potential lateral spread between infected premises, a key factor in understanding threat to the commercial sector.
Contextual and temporal regulation of fear memory consolidation in the basolateral amygdala complex
Authors: Leake Jessica , Cardona Luisa Saavedra , Westbrook R. Frederick , Holmes Nathan M. 
Published: December 2022

AbstractIt is widely accepted that fear memories are consolidated through protein synthesis-dependent changes in the basolateral amygdala complex (BLA). However, recent studies show that protein synthesis isnotrequired to consolidate the memory of a new dangerous experience when it is similar to a prior experience. Here, we examined whether the protein synthesis requirement for consolidation of the new experience varies with its spatial and temporal distance from the prior experience. In each experiment, rats were conditioned to fear a stimulus (S1, e.g., light) across its pairings with shock in stage 1; and a second stimulus (S2, e.g., tone) that preceded additional S1-shock pairings (S2-S1-shock) in stage 2. The latter stage was followed by a BLA infusion of a protein synthesis inhibitor, cycloheximide or vehicle. Finally, rats were tested for fear to S2. Critically, protein synthesis in the BLA wasnotrequired to consolidate fear to S2 when the two training stages occurred 48 hours apart and in the same context;wasrequired when the two training stages were separated by a 14-day delay or occurred in different contexts; but wasagain notrequired when rats were re-exposed to S1 or shock after the delay or in the different context. Thus, protein synthesis in the BLA is not always required to consolidate a new fear memory. Instead, this requirement is determined by the degree of similarity between present and past experiences, the time and place in which those experiences occur, as well as reminders of the past experience.Significance StatementProtein synthesis in the basolateral amygdala complex (BLA) isnotrequired to consolidate the memory of a new dangerous experience when it is similar to a prior experience. This study is significant in showing that: 1) when the new, similar experience occurs after a delay or in a different context, the protein synthesis requirement for its consolidation is reinstated; and 2) the effects of the delay and context shift are reversed by reminding animals of their prior experience. Thus, the neural mechanisms underlying memory consolidation are dynamically regulated by similarity/dissimilarity between present and past experiences, the time and place in which those experiences occur, as well as reminders of the past experience.
Cytokinin synthesis and export from symbiotic root nodules coordinates shoot growth with nitrogen fixation
Authors: Chen Yumeng , Liu Jie , Lin Jieshun , Roswanjaya Yuda Purwana , Nadzieja Marcin , Buron Flavien , Kohlen Wouter , Geisler Markus , Stougaard Jens , Reid Dugald 
Published: December 2022

AbstractDevelopment of symbiotic root nodules is a cytokinin-dependent process that is critical to nitrogen acquisition in legumes. The extent and manner in which root nodules contribute to whole-plant cytokinin and nitrogen supply signalling is unknown.Using a combination of genetic, biochemical and physiological approaches, we characterised the role of cytokinin synthesis, export and perception in coordination of symbiotic nodule development and shoot growth in the legumeLotus japonicus.LjPup1encodes a plasma membrane localised cytokinin exporter with isopentenyladenine (iP) andtrans-Zeatin (tZ) export capacity.LjPup1shows a distinct nodule-specific expression pattern with greatest transcript levels detected in mature nodules. Mutants accumulate more isopentenyladenine riboside (iPR) in nodule tissues and demonstrate hallmarks of reduced cytokinin signalling. Despite normal nodule numbers and function, shoot growth is markedly reduced inLjpup1mutants, as well as in mutants impaired in tZ biosynthesis.We found symbiotic root nodules contribute to shoot growth via export of active cytokinins. A cytokinin exporter in the purine permease family thus contributes to long-distance cytokinin homeostasis regulating plant development.
Spatial single-cell mass spectrometry defines zonation of the hepatocyte proteome
Authors: Rosenberger Florian A. , Thielert Marvin , Strauss Maximilian T. , Ammar Constantin , Mädler Sophia C. , Schweizer Lisa , Metousis Andreas , Skowronek Patricia , Wahle Maria , Gote-Schniering Janine , Semenova Anna , Schiller Herbert B. , Rodriguez Edwin , Nordmann Thierry M. , Mund Andreas , Mann Matthias 
Published: December 2022

AbstractSingle-cell proteomics by mass spectrometry (MS) is emerging as a powerful and unbiased method for the characterization of biological heterogeneity. So far, it has been limited to cultured cells, whereas an expansion of the method to complex tissues would greatly enhance biological insights. Here we describe single-cell Deep Visual Proteomics (scDVP), a technology that integrates high-content imaging, laser microdissection and multiplexed MS. scDVP resolves the context-dependent, spatial proteome of murine hepatocytes at a current depth of 1,700 proteins from a slice of a cell. Half of the proteome was differentially regulated in a spatial manner, with protein levels changing dramatically in proximity to the central vein. We applied machine learning to proteome classes and images, which subsequently inferred the spatial proteome from imaging data alone. scDVP is applicable to healthy and diseased tissues and complements other spatial proteomics or spatial omics technologies.
Visual homogeneity computations in the brain enable solving property-based visual tasks
Authors: Jacob Georgin , Pramod R. T. , Arun S. P. 
Published: December 2022

ABSTRACTMost visual tasks involve looking for specific object features. But we also often perform property-based tasks where we look for specific property in an image, such as finding an odd item, deciding if two items are same, or if an object has symmetry. How do we solve such tasks? These tasks do not fit into standard models of decision making because their underlying feature space and decision process is unclear. Using well-known principles governing multiple object representations, we show that displays with repeating elements can be distinguished from heterogeneous displays using a property we define as visual homogeneity. In behavior, visual homogeneity predicted response times on visual search, same-different and symmetry tasks. Brain imaging during visual search and symmetry tasks revealed that visual homogeneity was localized to a region in the object-selective cortex. Thus, property-based visual tasks are solved in a localized region in the brain by computing visual homogeneity.SIGNIFICANCE STATEMENTMost visual tasks involve looking for specific features, like finding a face in a crowd. But we also often look for a particular image property – such as finding an odd item, deciding if two items are same, or judging if an object is symmetric. How does our brain solve these disparate tasks? Here, we show that these tasks can all be solved using a simple computation over object representations in higher visual cortex, which we define as visual homogeneity.
Characterizing top-down microcircuitry of complex human behavior across different levels of the visual hierarchy
Authors: Dowdle Logan , Ghose Geoffrey , Moeller Steen , Ugurbil Kamil , Yacoub Essa , Vizioli Luca 
Published: December 2022

AbstractFMRI has become a key tool for human neuroscience. At ultra-high field (=> 7 Tesla) it is possible to acquire images with submillimeter spatial precision, which allows examinations of mesoscale functional organization. Studying the brain at this scale does not come without its challenges, however. To tackle some of these challenges, we propose an approach that builds upon task modulations to identical visual stimuli and the simultaneous imaging of distal areas of varying complexity across the cortical hierarchy. Specifically, we record BOLD responses elicited by face stimuli during a stimulus relevant and a stimulus irrelevant task across cortical depths in V1, Occipital Face (OFA) and Fusiform Face area (FFA). We observed that task-related top-down modulations were larger in the inner compared to the outer layers of V1; and in the outer compared to the inner layers in the FFA. Our findings are consistent with animal reports of feedback exchange between deeper and superficial layers and with the notion of apical dendritic amplification as a key mechanism of conscious perception. Our approach showcases the potential of “laminar-fMRI” to explore large scale network activity and represents a promising step towards characterizing laminar functional profiles in humans for complex, cognitively meaningful, and socially relevant stimuli such as faces.
Cultural signatures of punctuated environmental change: measuring the effects of population-wide stress on song distribution in dark-eyed juncos and song sparrows
Authors: Snyder Kate T. , Sellers Maria L. , Creanza Nicole 
Published: December 2022

AbstractSevere weather events can dramatically alter a species’ evolutionary trajectory. Previous landmark studies on populations in transiently hostile environments have measured traits that are under direct selection during those events. However, phenotype shifts that are not inherently adaptive to the fluctuating environment may also occur. Stress, especially during development, can cause important phenotypic changes in individuals, including impaired learning. Thus, learned behaviors, such as birdsong, may exhibit unique evolutionary dynamics as a result of widespread environmental stress. We hypothesize that ecosystem-level stressors may cause population-level changes to birdsong as entire cohorts experience developmental stress, learn songs imperfectly, and become the song tutors for future generations. In 2016, an unprecedented drought affected western New York State, a hotspot for community-science-generated birdsong recordings, and presented a unique opportunity to test our hypothesis through a natural experiment. We analyzed publicly available community-science birdsong recordings of two species,Junco hyemalisandMelospiza melodia, recorded between 2006-2020 in the drought-affected region and two control regions. We found that population-level song features had changed in the species with more complex songs (song sparrow) in the drought area after 2016, but not in the control area or in the species with a simple song (dark-eyed junco), implying that stress-induced deficits may disproportionately affect song traits that are more difficult to learn. These results demonstrate that environmental events can drive population-level trait evolution due to disruption in learning, with potentially important implications for speciation.
Apixaban following discharge in hospitalised adults with COVID-19: Preliminary results from a multicentre, open-label, randomised controlled platform clinical trial
Authors: Toshner Mark R , Gamble Carrol , Baillie J Kenneth , Best Ashley , Bedson Emma , Bradley Judy , Calvert Melanie , Davies Elin Haf , Docherty Annemarie B , Gkioni Efstathia , Hughes Dyfrig A , Jaki Thomas , Jenkins R Gisli , Jones Ashley , Landray Martin J , Mant Jonathan , McAuley Daniel F , Openshaw Peter JM , Richards Duncan , Wicks Paul ,   HEAL-COVID Collaboration, Summers Charlotte 
Published: December 2022

SummaryBackgroundThe role of thromboprophylaxis in the post-acute phase of COVID-19 is uncertain due to conflicting results from randomised controlled trials and observational studies. We aimed to determine the effectiveness of post-hospital apixaban in reducing the rate of death and hospital readmission of hospitalised adults with COVID-19.MethodsHEAL COVID is an adaptive randomised open label multicentre platform trial recruiting participants from National Health Service Hospitals in the United Kingdom. Here we report the preliminary results of apixaban comparison of HEAL-COVID. Participants with a hospital admission related to confirmed COVID-19 and an expected date of discharge in the subsequent five days were randomised to either apixaban 2.5 mg twice daily or standard care (no anticoagulation) for 14 days. The primary outcome was hospital free survival at 12 months obtained through routine data sources. The trial was prospectively registered with ISRCTN (15851697) andClincialtrials.gov(NCT04801940).FindingsBetween 19 May 2021 and 21 November 2022, 402 participants from 109 sites were randomised to apixaban and 399 to standard care. Seven participants withdrew from the apixaban group and one from the standard care group. Analysis was undertaken on an intention-to-treat basis.The apixaban arm was stopped on the recommendation of the oversight committees following an interim analysis due to no indication of benefit. Of the 402 participants randomised to apixaban, 117 experienced death or rehospitalisation during a median follow-up of 344·5 days (IQR 125 to 365), and 123 participants receiving standard care experienced death or rehospitalisation during a median follow-up of 349 days (IQR 124 to 365). There was no statistical difference in the rate of death and rehospitalisation (HR: 0·96 99%CI 0·69-1·34; p=0·75). Three participants in the apixaban arm experienced clinically significant bleeding during treatment.InterpretationFourteen days of post-hospital anticoagulation with the direct oral anticoagulant apixaban did not reduce the rate of death or rehospitalisation of adults hospitalised with COVID-19. These data do not support the use of prophylactic post-hospital anticoagulation in adults with COVID-19.FundingHEAL-COVID is funded by the National Institute for Health and Care Research [NIHR133788] and the NIHR Cambridge Biomedical Research Centre [BRC-1215-20014*].
What counts, safety and security or hygiene? Suggestions on the reopening of major events under pandemic conditions in Germany
Authors: Schütte Patricia Magdalena , Schönefeld Malte , Schulte Yannic , Fiedrich Frank 
Published: December 2022

PurposeBetween 2020 and spring 2022, health safety was the new pressing concern among the risks at major events. It seemed that it – respectively hygiene as part of infection control – was as important as event safety if an event in Germany was to be approved. Problems aroused in terms of an equal implementation in practice. This article therefore addresses how safety and hygiene aspects interacted during event planning and implementation phases.Design/methodology/approachThe authors draw on qualitative data from a German research project. They use results from eleven semi-structured expert interviews and four field observations at major events. One guiding assumption in the content analysis is that there are major interrelations between event and health safety concepts, which become visible during planning and the implementation of event-related technical, organisational and personal measures.FindingsThe empirical data shows that hygiene is not perceived as an integral part of event safety, but rather as a disconnected pillar beside the “classical” event safety. This is reflected in an imbalanced attention as well as in separate, disintegrated concepts. This disconnectedness leaves room for unwanted interplays between event and health safety as well as potential legitimacy facades.Originality/valueMost studies to date focus on the effectiveness of hygiene concepts and impacts of COVID-19 on the event sector in general without taking a closer look at interactions between event safety and health safety.
Do populist parties in government produce unconstitutional policies? Evidence from Austria, 1980–2021
Authors: KÖNIG JASMIN SARAH , SWALVE TILKO 
Published: December 2022

AbstractIn a rising number of countries, populist parties participate in coalition governments. While there exists a consensus that populism is incompatible with core tenets of liberal democracy on a conceptual level, we know much less about whether or not the participation of populist parties in government constitutes a threat to liberal democracy in practice. We study the impact of populist parties in coalition governments using a novel dataset of more than 2000 laws that were under review at the Austrian Constitutional Court between 1980 and 2021. We provide evidence that the court did not find laws passed by governments that included a populist party unconstitutional more often than those passed by non‐populist governments. Our findings indicate that the Austrian Freedom Party did moderate its policy while in office. The results imply that it is necessary to distinguish policy positions in party manifestos and party rhetoric from policies actually implemented by governments.
Inhibition of spoilage bacteria on marinated chicken by essential oils under aerobic and vacuum packaging
Authors: Osaili Tareq M. , Al‐Nabulsi Anas A. , Hasan Fayeza , Dhanasekaran Dinesh K. , Ismail Leila Cheikh , Hashim Mona , Hasan Hayder , Ayyash Mutamed , Olaimat Amin , Hussain Ayman Z. S. , Darra Nada El , Savvaidis Ioannis N. , Obaid Reyad S. , Holley Richard 
Published: December 2022

Abstract“Chicken tawook” is a marinated boneless chicken entrée consumed in the Middle East. The aim of this study was to determine whether bioactive essential oil (EO) components carvacrol (CA), cinnamaldehyde (CI), and thymol (TH) would delay the growth of microorganisms causing tawook spoilage during aerobic (AP) or vacuum (VP) packed storage. The EOs at 1% and 2% were mixed individually with the marinade. The samples (10 g of chicken cubes with 1.2 g of marinade – with or without EOs) were stored in bags under AP and VP (Geryon®) for 7 days at 4 ± 1°C and abusive conditions (10 ± 1°C). Two control samples consisting of meat chunks and tawook without EO were used. The microflora numbers were greater at 10°C than at 4°C, and the marinade worked additively with AP against anaerobes, yeast and mold (Y & M) and lactic acid bacteria. It also worked additively with VP against aerobic bacteria recovered as Pseudomonas and the total plate count. EO components were observed to decrease microbial populations by a maximum of 4 to 6 log colony‐forming unit (CFU)/g depending on the type of microorganism. The combined mixture of marinade and 2% EO (CA, CI, and TH) resulted in the greatest reductions of all spoilage microorganisms at 10°C under AP on the last day of storage. Overall, VP was more effective (p < 0.05) than AP in controlling microorganisms at both 4 and 10°C. This study provides an affordable and natural alternative for extending product life.Practical ApplicationThe use of EOs in marinated chicken (tawook) is expected to help producers reduce spoilage and extend shelf‐life of the product when stored at refrigeration temperatures. EOs provide a cheaper alternative and are naturally sourced. Vacuum packaging will increase the shelf‐life of marinated chicken tawook and facilitate its storage and transportation.
Optimization of spray drying conditions for improved physical properties in the production of enzyme‐modified cheese powder
Authors: Salum Pelin , Berktas Serap , Bas Deniz , Cam Mustafa , Erbay Zafer 
Published: December 2022

AbstractEnzyme‐modified cheese (EMC), a cheese flavor additive with high‐fat content, is preferably produced in powder form because of its long shelf‐life and high industrial applicability. The physical properties of additives, especially with high‐fat contents, are very important for their industrial usage, and the spray drying process conditions substantially determine the physical properties of powders. In this study, optimization of the spray drying process during the production of EMC powder was performed to improve the powder physical properties. The process factors were inlet temperature, feed flow rate, and aspiration rate, while the responses were selected as drying yield, Carr index (CI), wettability, surface fat content, and browning index (BI). The optimum spray drying conditions were calculated as 150°C, 9.1 mL/min, and 28.4 m3/h for inlet temperature, feed flow rate, and aspiration rate, respectively. It has been determined that the spray drying conditions at low inlet temperature, medium feed flow, and aspiration rates in order to obtain improved powder physical properties should be preferred.Practical Application: Enzyme‐modified cheese (EMC) is a widely used product in the development of foods with cheese flavor, and EMC in powder form offers various advantages for industrial applications such as ease in storage and transportation, long shelf‐life, and product applicability, which mainly depend on powder physical properties. In powder production, spray drying is the principle process determining the powder physical properties, and optimization is essential for the desired physical properties. In this study, laboratory‐scale optimization of EMC powder production was carried out, data was provided for scale‐up studies, and the effects of processing conditions on powder physical properties were evaluated.