bluuebunny/crossref_metadata_2025_split huggingface dataset:
The nucleoporin NUP160 and NUP96 regulate nucleocytoplasmic export of mRNAs and participate in ethylene signaling and response in Arabidopsis
Authors: Nie Yuanyuan , Li Yang , Liu Menghui , Ma Binran , Sui Xinying , Chen Jiacai , Yu Yanchong , Dong Chun-Hai 
Published: December 2022

Abstract
The two-way transport of mRNAs between the nucleus and cytoplasm are controlled by the nuclear pore complex (NPC). In higher plants, the NPC contains at least 30 nucleoporins. The Arabidopsis nucleoporins are involved in various biological processes such as pathogen interaction, nodulation, cold response, flowering, and hormone signaling. However, little is known about the regulatory functions of the nucleoporin NUP160 and NUP96 in ethylene signaling pathway. In the present study, we provided data showing that the Arabidopsis nucleoporin NUP160 and NUP96 participate in ethylene signaling-related mRNAs nucleocytoplasmic transport. The Arabidopsis nucleoporin mutants (nup160, nup96-1, nup96-2) exhibited enhanced ethylene sensitivity. Nuclear qRT-PCR analysis and poly(A)-mRNA in situ hybridization showed thatthe nucleoporin mutants affected the nucleocytoplasmic transport of all the examined mRNAs, including the ethylene signaling-related mRNAs such as ETR2, ERS1, ERS2, EIN4, CTR1, EIN2, and EIN3. Transcriptome analysis of the nucleoporin mutants provided clues suggesting that the nucleoporin NUP160 and NUP96 may participate ethylene signaling via various molecular mechanisms. These observations significantly advance our understanding of the regulatory mechanisms of nucleoporin proteins in ethylene signaling and ethylene response.
How fine root turnover functions during mangrove root zone expansion and affects belowground carbon processes
Authors: Lin Qiulian , Chen L.Z. , Zhang Jialin , Wang Liangchen , Yu Xinyue , Guo Qiang 
Published: December 2022

Abstract
Background and aims
Fine roots help to maintain sediment volume and resist soil compaction in mangroves. In addition, fine root turnover contributes to belowground carbon stocks. This study focused on root zone dynamics, and aimed to quantify the composition of live and dead fine roots and analyze their functions during root zone expansion and belowground carbon accumulation.
Methods
Shallow surface elevation tables for measuring root zone expansion were set up in Dongzhaigang Bay of Hainan Province, China; root cores and in-growth bags for measuring fine root biomass and turnover rates were used in four typical mangrove forests.
Results
Fine root biomass contributed over 60% to belowground roots, and was mainly composed of up to 69.25% dead fine roots. Fine root productivity ranged from 1.97 to 5.30 t ha− 1 yr− 1 and did not showed significantly difference among these forests. Fine root turnover rates ranged from 6–14% per year within the four forests, showing the fastest in Bruguiera forest, followed by Kandelia forest, Sonneratia plantation and Rhizophora forest. Root zone expansion rates ranged from 0.55 to 1.28 mm yr− 1, and was positively related to live fine root biomass within the upper 50 cm layer of sediment in the four forests types (R2 = 0.625, p = 0.0022).
Conclusions
Live fine root biomass took up less than 30.75% of belowground biomass, but remarkably supported 62.5% of root zone expansion in mangroves. Turnover rates of fine roots significantly contributed to the highly dynamic changes in the carbon processes of subsurface sediment.
A new special femoral neck osteotomy method for application in the surgical treatment of patients with ankylosing spondylitis and severe thoracolumbar kyphosis combined with hip flexion contracture
Authors: Yang Xin , Wang Qiwei , Meng Zhicao , Liu Heng , Wu Hao , Juma Talante , Wang Yu , Cao Yongping 
Published: December 2022

Abstract
Background
Surgeons often experience difficulty operating on patients with ankylosing spondylitis who have thoracolumbar kyphosis combined with hip fusion. Femoral osteotomy is used when performing pedicle subtraction osteotomy (PSO) and total hip arthroplasty (THA). We evaluate the effect of a new special femoral neck osteotomy (FNO) method in the surgical treatment of patients with ankylosing spondylitis and severe thoracolumbar kyphosis combined with hip flexion contracture.
Methods
We included 5 male patients with 9 bone-fused hips who underwent the new femoral neck osteotomy from October 2021 to March 2022. We designed the saw blade to be manipulated from the lateral femoral neck base to the inferior part of the femoral head, keeping Pauwels’ angle less than 30° on the coronal plane. On the transverse plane, the angle between the saw blade and the coronal plane was more than 15°. On the sagittal plane, the saw blade cut through the femoral neck.
Results
The average operation time and blood loss of FNO, the average interval between FNO and THA were showed. The average angle of the trunk and lower limb (ATL) was 36.33 ± 16.36° pre-FNO, 82.89 ± 13.51° post-FNO and 175.22 ± 3.42° post-THA. The average visual analogue scale (VAS) scores was 0 pre-FNO, 5 ± 1.58 post-FNO and 2.6 ± 0.55 post-THA. The average Harris hip score (HHS) was 43.56 ± 1.59 preoperatively and 83.89 ± 2.21 postoperatively. The average hip extension range of motion (ROM) was 23.89 ± 12.69° pre-FNO, -22.67 ± 14.18° post-FNO and − 3.33 ± 2.50 post-THA°. The average hip flexion ROM was 23.89 ± 12.69° pre-FNO, 35.56 ± 12.11° post-FNO and 104.44 ± 5.27° post-THA. The differences among them were significant (P < 0.05). Only one hip (11.11%) was dislocated after FNO.
Conclusions
We developed a new special FNO, which can provide osteotomy with a certain degree of stability and greater ease for performing PSO and THA.
Factors associated with professional identity of rehabilitation therapy students in China:A cross-sectional study
Authors: Zheng Yan-xue , Tao Huan 
Published: December 2022

Abstract
Objective To investigate the current situation and factors influencing the professional identity of rehabilitation therapy students through questionnaires, and to provide reference for improving students' professional identity and proposing effective coping strategies.
Methods A questionnaire survey was conducted on 189 rehabilitation students in a university in Gansu Province using general information questionnaire and professional identity scale; SPSS26.0 software was used to analyze the data statistically.
ResultsThe total score of professional identity of rehabilitation therapy students was (84.76±12.30), and the scores of each dimension were from highest to lowest: affective>cognitive>behavioral>appropriateness.
Conclusion The professional identity of rehabilitation therapy students is at a moderate to high level, and the influencing factors include students' education and the way they choose their profession.
Clinicopathological characteristics and prognosis of pregnancy-associated breast cancer: a multicenter retrospective study from the central China
Authors: Liu Chenguang , Liu Shiyang , Yu Tianyao , Wu Xinhong , Zheng Weihong , Li Shuyu , Yao Tian , Gui Zhangwei , Zhang Lin 
Published: December 2022

Abstract
Purpose: This study aimed to compare the demographic and clinical characteristics of pregnancy-associated breast cancer (PABC) and non-PABC patients, and to analyze the prognostic factors.
Methods: PABC patients ≤ 40 years old who were treated in three large tertiary institutions from January 1, 2016 to August 1, 2020 were included. The population and tumor characteristics of the patients were collected, and their survival were followed up. For each case, a non-PABC patient was matched as a control.
Results: A total of 69 PABC patients and 69 non-PABC patients were included in the study. Compared with the non-PABC group, the clinical stage of PABC patients was later. In the univariate and multivariate analysis of all patients with breast cancer, it was found that the prognosis of PBAC patients was worse than that of controls and pregnancy correlation was not an independent factor affecting the prognosis of breast cancer. Moreover, there was no significant difference in clinicopathological characteristics between pregnancy and postpartum breast cancer. In the analysis of prognostic factors of PABC patients, we found that gestational weight gain (GWG) and vascular invasion were important factors affecting disease-free survival (DFS) of PABC patients, while oral contraceptive and clinical stage were important factors affecting their overall survival (OS).
Conclusions: The prognosis of PABC patients was worse. However, pregnancy correlation was not an independent factor affecting the prognosis of breast cancer patients. Vascular invasion and inadequate or excessive gestational weight gain were important factors affecting recurrence and metastasis in PABC patients. Generally speaking, it is necessary to pay more attention to weight gain during pregnancy.
Surface Properties of Molds for Powder Injection Molding and Their Effect on Feedstock Moldability and Mold Adhesion
Authors: Demers Vincent , Kurusu Rafael S. , Gholami Mohsen , Demarquette Nicole R. 
Published: December 2022

Abstract
The surface energy of various mold materials for low-pressure powder injection molding was evaluated using values of contact angles (Owens-Wendt method), and correlated with the feedstock moldability and mold adhesion. The surface tension of the binder used to formulate a metallic-based feedstock was also measured in the molten state at a typical injection temperature using the pendant drop technique. Real-scale injection tests were performed into metallic and polymeric mold cavities to assess the feedstock moldability and its adhesion with the mold surfaces that were compared with theoretical predictions obtained from the surface energies values. The results confirmed that the adhesion was significantly affected by the interfacial energy between the mold and the binder - in this case, the metallic mold exhibited low adhesion as compared to the polymeric mold. It was finally demonstrated that the adhesion phenomenon is only related to the surface properties of the mold (i.e., they are not related to the solidification rate) - in this case, a gold-coated polymeric mold produced the moldability of a polymeric mold and the adhesion properties of a metallic mold, which translated into a high moldability potential, with no resulting adhesion of the feedstock with the mold.
Endoscopic third ventriculostomy for management of hydrocephalus associated with Chiari malformation type II in children
Authors: El-Ghandour Nasser M. F. , Salama Mohamed M , Ghoneim Mohamed Adel , Attia Ahmed M. 
Published: December 2022

Abstract
Background: Hydrocephalus is commonly associated with Chiari malformation (CM) particularly CM type II. The traditional treatment of hydrocephalus in these patients has been cerebrospinal fluid diversion by shunts. Endoscopic third ventriculostomy (ETV) has emerged as an alternative procedure in these patients.
Purpose: Assessment of the clinical and radiological outcomes of ETV in the management of hydrocephalus in children with CM II.
Methods: This is a prospective study conducted on 18 patients with CM II associated with hydrocephalus admitted to Cairo University hospitals between January 2020 and June 2021. These patients had been managed surgically by ETV. Clinical outcome was assessed based on improvement of manifestations of increased intracranial pressure while radiological outcome was based on the findings of postoperative computed tomography. In cases with early failure, serial lumbar puncture (LP) was performed for 2 days.
Results: ETV was performed as a secondary procedure in 4 cases. The overall success rate of the procedure was 72%, and its success rate as a secondary procedure was 100%. Serial LP was effective in decreasing early failure in 44.4% of cases. Radiological regression of hydrocephalic changes was detected in 50% of the cases.
Conclusion: ETV is an efficient and safe procedure in treatment of hydrocephalus in children with Chiari malformation II, particularly when performed as a secondary procedure. Serial LP following the procedure increases the success rate in patients with early failure.
Inhalable SARS-CoV-2 vaccines for single-dose dry-powder aerosol immunization and orchestrated mucosal/systemic immune responses
Authors: Ye Tong , Jiao Zhouguang , Li Xin , He Zhanlong , Li Yanyan , Yang Fengmei , Zhao Xin , Wang Youchun , Huang Weijin , Qin Meng , Feng Yingmei , Qiu Yefeng , Yang Wenhui , Hu Lingfei , Hu Yaling , Zhai Yu , Wang Erqiang , Yu Di , Wang Shuang , Yue Hua , Wang Hengliang , Zhu Li , Ma Guanghui , Wei Wei 
Published: December 2022

Abstract
The ongoing coronavirus disease pandemic has fostered major advances in vaccination technologies; however, there are urgent needs of mucosal immune responses and single-dose, non-invasive administration. Here, we develop a SARS-CoV-2 vaccine for single-dose, dry-powder aerosol inhalation that induces potent systemic and mucosal immune responses. Our vaccine encapsulates proteinaceous cholera toxin B subunit-assembled nanoparticles displaying the SARS-CoV-2 RBD antigen (R-CNP) within microcapsules of optimal aerodynamic size, and such unique nano-micro coupled structure supports efficient alveoli delivery, sustained R-CNP release, and antigen presenting cell uptake, which are favorable for invocation of immune responses. Moreover, our vaccine successfully induces robust serological IgG and secretory IgA production, collectively conferring effective protection from SARS-CoV-2 challenge (including pseudovirus and the authentic virus) in mice, hamsters, and non-human primates. Finally, we also demonstrate a “mosaic iteration” of our vaccine that co-displays ancestral and Omicron’s antigens, thus extending the breadth of antibody response against co-circulating strains and transmission of Omicron variant. These findings support our inhalable vaccine as a promising candidate to prevent SARS-CoV-2 infection, disease, and transmission.
Conventional and laser-assisted machining of laser-borided Monel 400 alloy
Authors: Przestacki Damian , Kukliński Mateusz , Bartkowska Aneta , Kieruj Piotr , Radek Norbert 
Published: December 2022

Abstract
This study concentrates on comparing effects of conventional and laser-assisted machining (LAM) of laser borided Monel 400 alloy. For this purpose, the shaft made from Monel 400 was covered with 200 µm thick boron layer and surface melted using diode laser. For determining the influence of laser beam scanning velocity on final microstructure, microhardness and depth of melting, four laser beam scanning velocities were set: 5, 6, 8 and 10 m/min. Obtained microstructures are typical for laser melted metal and are composed of fine crystals. Microhardness increased significantly due to enriching Monel 400 with boron and the level of this increase depends on laser beam scanning velocity carried out for the process. During both conventional and laser-assisted turning cutting forces were measured for comparison. Other compared parameters after carrying out these processes were: obtained surface roughness and tool’s life. It was found that it is possible to form laser borided surface of Monel 400 alloy by machining and using additional laser-assist has a positive impact on the efficiency of the process. The paper is the first description of effects obtained by laser assisted machining of laser borided surface.
Extrinsic Arterial Compression and Lower Extremity Ischemia After Iliac Vein Stent Placement: Case Report and Review of Literature
Authors: Elsayed Mohammad , Sarkar Debkumar , Zhao Ken , Bryce Yolanda , Friedman Adie 
Published: December 2022

Abstract
Background
Lower extremity ischemia due to extrinsic arterial compression by venous stent placement is a rare but increasingly recognized occurrence. Given the rise of complex venous interventions, awareness of this entity is becoming increasingly important to avoid serious complications.
Case Presentation
A 26-year-old with progressively enlarging pelvic sarcoma despite chemoradiation developed recurrent symptomatic right lower extremity deep venous thrombosis due to worsening mass effect on a previously placed right common iliac vein stent. This was treated with thrombectomy and stent revision, with extension of the right common iliac vein stent to the external iliac vein. During the immediate post-procedure period the patient developed symptoms of acute right lower extremity arterial ischemia including diminished pulses, pain, and motorsensory loss. Imaging confirmed extrinsic compression of the external iliac artery by the newly placed adjacent venous stent. The patient underwent stenting of the compressed artery with complete resolution of ischemic symptoms.
Conclusions
Awareness and early recognition of arterial ischemia following venous stent placement is important to prevent serious complication. Potential risk factors include patients with active pelvis malignancy, prior radiation, or scarring from surgery or other inflammatory processes. In cases of threatened limb, prompt treatment with arterial stenting is recommended. Further study is warranted to optimize detection and management of this complication.
Ibrutinib in combination with rituximab is highly effective in treatment of steroid refractory autoimmune hemolytic anemia
Authors: Nikitin Eugene , Kislova Maria , Morozov Dmitry , Belyakova Vera , Suvorova Anna , Sveshnikova Julia , Vyscub Galina , Matveeva Irina , Shirokova Maria , Shipaeva Anna , Klitochenko Tatyana , Dmitrieva Elena , Biderman Bella , Sudarikov Andrey , Obukhova Tatyana , Samoilova Olga , Kaplanov Kamil , Konstantinova Tatyana , Mayorova Olga , Poddubnaya Irina , Ptushkin Vadim 
Published: December 2022

Abstract
Autoimmune hemolytic anemia (AIHA) and pure red cell aplasia (PRCA) are common complications of CLL. The optimal treatment of steroid refractory AIHA/PRCA is not well established. We conducted a multicenter study of ibrutinib and rituximab in patients with relapsed/refractory to steroids AIHA/PRCA and underlying CLL. Protocol included induction (ibrutinib 420 mg/day and rituximab, 9 weekly and 3 monthly infusions) and maintenance phase with ibrutinib until progression or unacceptable toxicity. Fifty patients were recruited (44 – warm type AIHA, 2 – cold type AIHA, 4 – PRCA). After the induction phase 34 patients (74%) have achieved complete response, and 10 (21,7%) partial response. Median time to hemoglobin normalization was 85 days. With regards to CLL response 9 (19%) patients have achieved CR, 2 (4%) patients – stabilization and 39 (78%) – PR. The median follow-up was 37.56 months. In AIHA group 2 patients had a relapse. Among 4 patients with PRCA 1 patient did not respond, and 1 patient had a relapse after CR, 2 remained in CR. The most common adverse events were neutropenia (62%), infections (72%) and gastrointestinal complications (54%). In conclusion ibrutinib in combination with rituximab is an active second-line treatment option for patients with relapsed or refractory AIHA/PRCA and underlying CLL.
Factors affecting utilization of intimate partner violence services among women with disabilities
Authors: Namatovu Fredinah , Lövgren Veronica , Wickman Kim 
Published: December 2022

AbstractBackground:In the past few years several reports have indicated a high prevalence of intimate partner violence (IPV) among women with disabilities (WWDs), but there is limited research on access and utilization of IPV services among this population.Methods:Using qualitative in-depth data obtained from WWDs, our current study soughtto identify factors that hinder WWDs from accessing and utilizing IPV services.Results:Findings from this study showed barriers at different levels. At the individual level, the identity category of disability, not knowing about certain forms of violence, and being better off silent were the main explanations for not using IPV services. The role of personal connections was the main factor that influenced the utilization of IPV services, at the interpersonal level while at theorganizational level, a lack of effective communication, power imbalances, inadequate resources, and failures to follow response standards were identified as the major barriers to using IPV services. Societal incompetence, as exemplified by treating violence as a taboo and the lack of regulations criminalizing psychological violence, was the main barrier at the societal level.Conclusions:Our findings suggest that improving access to IPV services requires interventions that address barriers at different societal levels. This should include early interventions that target WWDs, as well as others, to promote violence recognition among service providers, improve the quality of services, and establish policies and programs that are not only directed to women with disabilities but also to interpersonal, organizational, and societal levels.
Endoscopic band ligation is safe despite low platelet count and high INR
Authors: Pfisterer Nikolaus , Schwarz Michael , Jachs Mathias , Hartl Lukas , Putre Florian , Ritt Lukas , Mandorfer Mattias , Madl Christian , Trauner Michael , Reiberger Thomas 
Published: December 2022

Abstract
Background Prophylactic endoscopic band ligation (EBL) is used to prevent variceal bleeding in patients with liver cirrhosis. The association of thrombocytopenia, high INR (international normalized ratio) and liver dysfunction with the risk of procedure-related bleeding (PRB) remains debated and recommendations are controversial.
Methods We analyzed real-life data of cirrhotic patients undergoing elective EBL at two Viennese centers between Q1/2000-Q1/2018. PRB was defined as bleeding occurring within 30 days after EBL.
Results We included 626 patients undergoing a total of 1519 prophylactic EBL procedures (median 2 per patient). Sixteen (2.6%) of 626 patients experienced PRB after a median of 12.5 (IQR 17.3) days with no difference in characteristics and laboratory values between the two groups. The proportion of patients with platelets (PLT) <50 G/L or INR≥1.5 was similar in patients with vs. without PRB. A higher MELD showed a nonsignificant association with EBL-related bleeding risk (odds ratio, OR: 1.07; 95% confidence interval 95%CI 1.00-3.11, p=0.058). While bilirubin level was a significant predictor for PRB (OR: 1.10; 95%CI: 1.03-1.18), the presence of large varices (OR: 0.90 vs. small varices; 95%CI: 0.20-4.05), INR (OR: 0.50; 95%CI: 0.10-3.11), PLT (OR: 1.00; 95%CI: 1.00-1.01) and the use of nonselective betablockers (OR: 1.19; CI95%: 0.38-3.75) were not associated with PRB.
Conclusion EBL is safe and procedure-related bleedings are rare (2.6%) including in patients with thrombocytopenia <50 G/L or high INR≥1.5. Only high MELD, and especially high bilirubin seems to be linked to increased EBL-related bleeding.