bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of propolis supplementation on irritable bowel syndrome with constipation (IBS‐C) and mixed (IBS‐M) stool pattern: A randomized, double‐blind clinical trial
Authors: Miryan Mahsa , Soleimani Davood , Alavinejad Pejman , Abbaspour Mohammadreza , Ostadrahimi Alireza 
Published: April 2022

AbstractBackgroundRecent evidence indicates that propolis can modulate gastrointestinal (GI) function. This trial aims to assess the efficacy of propolis supplementation on the severity of irritable bowel syndrome (IBS) symptoms.MethodsThis clinical trial was conducted on 56 subjects with IBS diagnosed by Rome IV criteria. Eligible subjects were randomly assigned to receive either 900 mg/day of propolis or matching placebo tablets for 6 weeks. The IBS symptom severity scale (IBS‐SSS) was used to evaluate IBS severity in five clinically applicable items.ResultsAfter adjusting anxiety scores, a significant reduction was observed in the overall score of IBS symptoms (−98.27 ± 105.44), the severity of abdominal pain (−24.75 ± 28.66), and the frequency of abdominal pain (−2.24 ± 3.51) with propolis treatment as compared to placebo (p‐value < .05). Patients in the propolis group were 6.22 times more likely to experience improvement in IBS symptoms than those in the placebo group (95% CI: 1.14–33.9; p‐value: .035). There was no significant change in anthropometric measurements and dietary intakes in both groups (p‐value > .05).ConclusionsOur results showed that propolis supplementation might have a beneficial effect on constipation subtype of IBS (IBS‐C) and mixed subtype of IBS (IBS‐M) severity by reducing the severity and frequency of abdominal pain in patients with irritable bowel syndrome (IBS).
Evolution of nonenzymatic browning during the simulated Msalais‐production process in models of grape juice
Authors: Zhang Rui‐li , Zhang Meng‐Meng , Pu Yun‐Feng , Zhu Li‐Xia 
Published: April 2022

AbstractMsalais is a wine fermented from boiled grape juice. Nonenzymatic browning (NEB) greatly affects the quality of Msalais, but to date its mechanism has not been systematically analyzed. In the current study, the evolution of NEB during Msalais production was investigated using models of grape juice. 5‐Hydroxymethylfurfural (5‐HMF), browning index (BI), yellowness index (YI), sample absorbance at 420 (A420), and b* increased during heating, with a clear transition point at 110 min. The Maillard reaction (MR) was the major contributor to NEB. Vitamin C (VC) facilitated NEB in the late stage of heating. During heating that lasted over 130 min, glucose contributed to NEB more than fructose, while the reverse was true for heating lasting less than 130 min. Proline (Pro) was the most important amino acid in facilitating NEB. BI and A420 decreased during fermentation, while increasing slightly during wine storage. In conclusion, this study identified the evolution of NEB during the Msalais process, which will facilitate the control of traditional Msalais production for improved wine quality.
Effect of color protection treatment on the browning and enzyme activity of Lentinus edodes during processing
Authors: Lin Tong , Zhou Zhiguo , Xing Chunmiao , Zhou Jiahui , Fan Gongjian , Xie Chunyan 
Published: April 2022

AbstractFresh Lentinus edodes (L. edodes) are prone to browning (including enzymatic and nonenzymatic browning), which affects their quality and leads to economic losses during later processing. This study explored various effective color protection methods (color protection reagent and/or blanching) for inhibiting the browning of L. edodes. First, a single‐factor experiment and a response surface method were used to optimize the concentration of the color retention reagent. The compound color retention reagent (comprising 0.1% phytic acid, 0.8% sodium citrate, and 0.5% d‐sodium erythorbate) had the smallest total color difference (ΔE) value, suggesting that the compound color reagent had a better inhibiting effect than the single agent. Following this, the blanching conditions were studied; the polyphenol oxidase (PPO) activity was the lowest when the blanching temperature was 90°C and blanching time 180 s, indicating that browning is likely to be minimal. Finally, comparing the oxidase activity and total color difference (ΔE) revealed that combining the two color protection methods inhibits browning better than using a single method (color protection reagent or blanching). In addition, the polysaccharide and vitamin C (VC) contents of L. edodes under optimal color protection conditions were determined, which were 0.96 and 2.54 g/100 g fresh weight (FW), respectively. The results demonstrated that this color protection method effectively inhibits browning, reduces the nutritional loss, and improves the quality of L. edodes.
Estimating survival after salvage surgery for recurrent salivary gland cancers: Systematic review
Authors: Mannelli Giuditta , Comini Lara V. , Sacchetto Andrea , Santoro Roberto , Spinelli Giuseppe , Bonomo Pierluigi , Desideri Isacco , Bossi Paolo , Orlandi Ester , Alderotti Giammarco , Franchi Alessandro , Palomba Annarita , Eccher Albino , Marchioni Daniele , Nocini Riccardo , Piazza Cesare , Molteni Gabriele 
Published: April 2022

AbstractRecurrent salivary gland carcinomas (RSCs) are poorly characterized and their clinical features and treatment options have not yet been fully described. The goal of this study was to analyze the therapeutic strategies and oncological outcomes of RSC patients through a literature review analysis. This systematic review was performed according to the PRISMA statements. Inclusion criteria for the systematic review were based on the population, intervention, comparison, and outcomes according to (PICO) framework. Two thousand seven hundred and four records were selected and 1817 recurrences were studied. Three hundred and sixty‐five patients underwent salvage surgery (20.1%) and their 5‐year mortality rate, overall survival and disease‐free survival were 35%, 70%, and 42%, respectively. RSCs are aggressive neoplasms with a high rate of distant metastases (28.9%). Salvage surgery can be considered in patients with limited local and/or regional recurrences, even in case of single distant relapse, appearing within the first 3 years of follow‐up.
Relationship between NAFLD and coronary artery disease: A Mendelian randomization study
Authors: Ren Zhewen , Simons Pomme I. H. G. , Wesselius Anke , Stehouwer Coen D. A. , Brouwers Martijn C. G. J. 
Published: April 2022

Background and Aims:
There is an ongoing debate on whether NAFLD is an active contributor or an innocent bystander in the pathogenesis of coronary artery disease (CAD). The aim of the present study was to assess the causal relationship between NAFLD and CAD.

Approach and Results:
We performed two‐sample Mendelian randomization (MR) analyses using summary‐level data to assess the association between genetically predicted NAFLD (i.e., chronically elevated serum alanine aminotransferase levels [cALT], imaging‐based and biopsy‐confirmed NAFLD) and risk of CAD. Analyses were repeated after exclusion of NAFLD susceptibility genes that are associated with impaired VLDL secretion.
Inverse‐variance weighted MR analyses showed a statistically significant association between genetically predicted cALT and risk of CAD (OR: 1.116, 95% CI: 1.039, 1.199), but not for the other NAFLD‐related traits (OR: 1.046, 95% CI: 0.764, 1.433 and OR: 1.014, 95% CI: 0.968, 1.062 for imaging‐based and biopsy‐confirmed NAFLD, respectively). MR‐Egger regression revealed a statistically significant intercept, indicative of directional pleiotropy, for all traits. Repeat analyses after exclusion of genes associated with impaired VLDL secretion showed consistent associations between genetically predicted NAFLD and CAD for all traits (i.e., cALT [OR: 1.203, 95% CI: 1.113, 1.300]), imaging‐based (OR: 2.149, 95% CI: 1.276, 3.620) and biopsy‐confirmed NAFLD (OR: 1.113, 95% CI: 1.041, 1.189), which persisted when more stringent biopsy‐confirmed NAFLD criteria were used (OR: 1.154, 95% CI: 1.043, 1.278) or when more stringent MR methods were applied. MR‐Egger regression did not show a statistically significant intercept.

Conclusion:
The two‐sample MR analyses showed a robust association between genetically predicted NAFLD and CAD after exclusion of genetic variants that are implicated in impaired VLDL secretion.
The short‐run and long‐run effects of trade openness on financial development: Some panel evidence for Europe
Authors: Caporale Guglielmo Maria , Sova Anamaria Diana , Sova Robert 
Published: April 2022

AbstractThis paper analyses the short‐ and long‐run effects of trade openness on financial development in a panel including data on 35 European countries over the period 2001–2019. For this purpose, it uses the pooled mean group (PMG) estimator for dynamic panels developed by Pesaran et al. (Journal of the American Statistical Association, 1999;94:621). The results differ depending on the income, governance and financial development level of the countries considered. In particular, it appears that in the middle‐income countries trade openness tends to strengthen financial development in the long run but to have an adverse effect in the short run. By contrast, in the case of high‐income countries with better institutions and a higher level of financial development, there is a positive and significant impact in the short run. Some policy implications of these findings are drawn.
Involvement of blood lncRNA UCA1 in sepsis development and prognosis, and its correlation with multiple inflammatory cytokines
Authors: Wang Jingmei , Feng Qiang , Wu Yiping , Wang Haiyan 
Published: April 2022

AbstractBackgroundSepsis is a highly life‐threatening disease. Long non‐coding RNA urothelial carcinoma associated 1 (lncRNA UCA1) participates in the processes of inflammation and organ injury in several diseases, whereas its role in sepsis patients is still unclear. The aim was to explore the clinical value of lncRNA UCA1 in sepsis patients.MethodsOne hundred seventy‐four sepsis patients and 100 age and gender‐matched controls were enrolled. LncRNA UCA1 in peripheral blood mononuclear cell samples was examined, and the level of inflammatory cytokines in serum samples was assessed.ResultsLncRNA UCA1 was highly expressed in sepsis patients compared with controls. LncRNA UCA1 was positively correlated with tumor necrosis factor‐α, interleukin (IL)‐6, IL‐17, intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 in sepsis patients, while it was not correlated with these inflammatory cytokines in controls. lncRNA UCA1 upregulation was related to raised APACHE II score and SOFA score in sepsis patients. Moreover, lncRNA UCA1 was increased in sepsis deaths compared with sepsis survivors and was independently correlated with increased 28‐day sepsis mortality risk. Further receiver operating characteristic curves presented that lncRNA UCA1 had a good value to predict 28‐motality risk, while its combination with other independent factors (including age, history of chronic kidney disease, G+ bacterial infection, Fungus infection, C‐reactive protein, and APACHE II score) exerted a great predictive value for 28‐day mortality risk.ConclusionLncRNA UCA1 is upregulated and correlates with multiple pro‐inflammatory cytokines, terrible disease severity, and poor prognosis in sepsis patients.
Frequency histograms of three high‐sensitivity cardiac troponin assays in a reference population
Authors: Cho Hanwool , Kim Hyunjung , Lee Jehoon , Kim Soo‐Young , Lee Hae Kyung , Kwon Hi Jeong , Kim Yeongsic 
Published: April 2022

AbstractBackgroundCardiac troponin (cTn) values above the 99th percentile upper reference limit (URL) indicate myocardial injury. We established 99th percentile URLs for three high‐sensitivity cTn (hs‐cTn) assays (Beckman Coulter Access hs‐cTnI, Abbott STAT hs‐cTnI, and Roche Elecsys hs‐cTnT) using a healthy population in Korea.MethodsEach cTn value was measured by three assays and analyzed by dividing by gender and age.ResultsThe frequency histograms of log‐transformed cTn values for Beckman and Abbott assays exhibited a bell‐shaped distribution. The 99th percentile URLs were 9.8, 17.4, and 17.3 ng/L in the total population; 10.9/9.0, 18.9/17.0, and 18.9/17.7 ng/L in the male/female population (p < 0.001 for all three assays); and 11.2/7.2, 19.9/14.5, and 22.7/9.3 ng/L in the older/younger population (p < 0.001 for all three assays) for Beckman, Abbott, and Roche assays, respectively.ConclusionAmong the three assays, bell‐shaped distributions were observed in a frequency histogram of log‐transformed cTn values for healthy population in Beckman and Abbott assays. Also, our findings show that the 99th percentile URLs for cTn levels vary not only by gender but age.
Fluorescence evidence of annexin A6 translocation across membrane in model matrix vesicles during apatite formation
Authors: Wang Yubo , Weremiejczyk Liliana , Strzelecka‐Kiliszek Agnieszka , Maniti Ofelia , Amabile Veschi Ekeveliny , Bolean Mayte , Ramos Ana Paula , Ben Trad Layth , Magne David , Bandorowicz‐Pikula Joanna , Pikula Slawomir , Millán Jose Luis , Bottini Massimo , Goekjian Peter , Ciancaglini Pietro , Buchet René , Dou Wei Tao , Tian He , Mebarek Saïda , He Xiao P. , Granjon Thierry 
Published: April 2022

AbstractMatrix vesicles (MVs) are 100–300 nm spherical structures released by mineralization competent cells to initiate formation of apatite, the mineral component in bones. Among proteins present in MVs, annexin A6 (AnxA6) is thought to be ubiquitously distributed in the MVs’ lumen, on the surface of the internal and external leaflets of the membrane and also inserted in the lipid bilayer. To determine the molecular mechanism(s) that lead to the different locations of AnxA6, we hypothesized the occurrence of a pH drop during the mineralization. Such a change would induce the AnxA6 protonation, which in turn, and because of its isoelectric point of 5.41, would change the protein hydrophobicity facilitating its insertion into the MVs’ bilayer. The various distributions of AnxA6 are likely to disturb membrane phospholipid organization. To examine this possibility, we used fluorescein as pH reporter, and established that pH decreased inside MVs during apatite formation. Then, 4‐(14‐phenyldibenzo[a,c]phenazin‐9(14H)‐yl)‐phenol, a vibration‐induced emission fluorescent probe, was used as a reporter of changes in membrane organization occurring with the varying mode of AnxA6 binding. Proteoliposomes containing AnxA6 and 1,2‐Dimyristoyl‐sn‐glycero‐3phosphocholine (DMPC) or 1,2‐Dimyristoyl‐sn‐glycero‐3phosphocholine: 1,2‐Dipalmitoyl‐sn‐glycero‐3‐phosphoserine (DMPC:DPPS 9:1), to mimic the external and internal MV membrane leaflet, respectively, served as biomimetic models to investigate the nature of AnxA6 binding. Addition of Anx6 to DMPC at pH 7.4 and 5.4, or DMPC:DPPS (9:1) at pH 7.4 induced a decrease in membrane fluidity, consistent with AnxA6 interactions with the bilayer surface. In contrast, AnxA6 addition to DMPC:DPPS (9:1) at pH 5.4 increased the fluidity of the membrane. This latest result was interpreted as reflecting the insertion of AnxA6 into the bilayer. Taken together, these findings point to a possible mechanism of AnxA6 translocation in MVs from the surface of the internal leaflet into the phospholipid bilayer stimulated upon acidification of the MVs’ lumen during formation of apatite.
The Janus face of development brokers across migration borders
Authors: Marabello Selenia 
Published: April 2022

AbstractEmploying an ethnographic approach, I analyse the agentive practices of a codevelopment project named Africoop, showing how it navigates the migration‐development field by blending egalitarianism rights‐based concerns with hierarchical political authority, foregrounding economic neoliberalism and translating development. Africoop advocated for migrants rights and opened up opportunities for social mobility and also reproduced gender asymmetries and power relations among Italian donors and Ghanaian recipients. By examining the organisational level of brokerage and the career trajectories of the main leaders, I unravel how social nets, aspirations and biographical paths are interwoven to reconfigure the brokers' representativeness and accountability across migration borders.
Serotonin 5‐HT7 receptor overexpression in the raphe nuclei area produces antidepressive effect and affects brain serotonin system in male mice
Authors: Rodnyy Alexander Ya. , Kondaurova Elena M. , Bazovkina Darya V. , Kulikova Elisabeth A. , Ilchibaeva Tatiana V. , Kovetskaya Alexandra I. , Baraboshkina Irina A. , Bazhenova Ekaterina Yu. , Popova Nina K. , Naumenko Vladimir S. 
Published: April 2022

AbstractHeterodimerization between 5‐HT7 and 5‐HT1A receptors seems to play an important role in the mechanism of depression and antidepressant drug action. It was suggested that the shift of the ratio between 5‐HT1A/5‐HT7 hetero‐ and 5‐HT1A/5‐HT1A homodimers in presynaptic neurons toward 5‐HT1A/5‐HT1A homodimers is one of the reasons of depression. Consequently, the artificial elevation of 5‐HT7 receptor number in presynaptic terminals might restore physiological homo‐/heterodimer ratio resulting in antidepressive effect. Here we showed that adeno‐associated virus (AAV)‐based 5‐HT7 receptor overexpression in the midbrain raphe nuclei area produced antidepressive effect in male mice of both C57Bl/6J and genetically predisposed to depressive‐like behavior ASC (antidepressant sensitive cataleptics) strains. These changes were accompanied by the elevation of 5‐HT7 receptor mRNA level in the frontal cortex of C57Bl/6J and its reduction in the hippocampus of ASC mice. The presence of engineered 5‐HT7 receptor in the midbrain of both mouse strains was further demonstrated. Importantly that 5‐HT7 receptor overexpression resulted in the reduction of 5‐HT1A receptor level in the membrane protein fraction from the midbrain samples of C57Bl/6J, but not ASC, mice. 5‐HT7 receptor overexpression caused an increase of 5‐HIAA/5‐HT ratio in the midbrain and the frontal cortex of C57Bl/6J and in all investigated brain structures of ASC mice. Thus, 5‐HT7 receptor overexpression in the raphe nuclei area affects brain 5‐HT system and causes antidepressive effect both in C57Bl/6J and in “depressive” ASC male mice. Obtained results indicate the involvement of 5‐HT7 receptor in the mechanisms underlying depressive behavior.
Altered basal ganglia infraslow oscillation and resting functional connectivity in complex regional pain syndrome
Authors: Lee Barbara , Di Pietro Flavia , Henderson Luke A. , Austin Paul J. 
Published: April 2022

AbstractComplex regional pain syndrome (CRPS) is a painful condition commonly accompanied by movement disturbances and often affects the upper limbs. The basal ganglia motor loop is central to movement, however, non‐motor basal ganglia loops are involved in pain, sensory integration, visual processing, cognition, and emotion. Systematic evaluation of each basal ganglia functional loop and its relation to motor and non‐motor disturbances in CRPS has not been investigated. We recruited 15 upper limb CRPS and 45 matched healthy control subjects. Using functional magnetic resonance imaging, infraslow oscillations (ISO) and resting‐state functional connectivity in motor and non‐motor basal ganglia loops were investigated using putamen and caudate seeds. Compared to controls, CRPS subjects displayed increased ISO power in the putamen contralateral to the CRPS affected limb, specifically, in contralateral putamen areas representing the supplementary motor area hand, motor hand, and motor tongue. Furthermore, compared to controls, CRPS subjects displayed increased resting connectivity between these putaminal areas as well as from the caudate body to cortical areas such as the primary motor cortex, supplementary and cingulate motor areas, parietal association areas, and the orbitofrontal cortex. These findings demonstrate changes in basal ganglia loop function in CRPS subjects and may underpin motor disturbances of CRPS.
Task management in decentralized autonomous organization
Authors: Zhao Xi , Ai Peilin , Lai Fujun , Luo Xin (Robert) , Benitez Jose 
Published: April 2022

AbstractIn the emerging platform economy, blockchain technologies are reshaping the digital economy. Moreover, disintermediation and decentralization have broken new ground for platform organizations and management mechanisms and instigated the concept of a DAO (Decentralized Autonomous Organization). Recent literature on operations management has called for further research on governance issues related to DAOs. In response to this call, we explore the relationship between DAO management efforts and platform performance in this study. Specifically, we propose and theoretically articulate decentralized voting tasks in DAOs as a new form of organizing. Harnessing both online and on‐chain data from seven sources, we empirically examine how voting task division, task allocation, reward distribution, and information provision affect platform performance in the context of MakerDAO (an Ethereum‐based stablecoin issuance platform). Our findings reveal that strategic decisions arrived at through voting have a positive impact on platform operational performance under certain conditions, whereas operational decisions resulting from voting have a negative impact. Moreover, we elucidate the moderating effects of voting task execution characteristics on the relationship between completed decision tasks and operational performance. These findings have important implications from both theoretical and practical perspectives. We also share all the raw data we use to promote the development of blockchain‐related empirical research.
Accurate screening of early‐stage lung cancer based on improved ResNeXt model combined with serum Raman spectroscopy
Authors: Leng Hongyong , Chen Cheng , Si Rumeng , Chen Chen , Qu Hanwen , Lv Xiaoyi 
Published: April 2022

AbstractScreening and diagnosis of early‐stage lung cancer is low worldwide, and when lung cancer progresses to late stage, it will greatly affect the treatment and survival time of patients. Therefore, the development of an affordable and accurate diagnostic technique is critical for lung cancer patients. In this study, we analyzed and verified the changes of substance composition in the serum of early‐stage lung cancer patients and proposed an improved ResNeXt model to achieve accurate classification of serum Raman spectra of lung cancer. The robustness of the model is improved by adding different decibels of Gaussian white noise and spectral offset to augment the data, trying to process the augmented data with various pre‐processing methods, and inputting the pre‐processed data into the improved ResNeXt model, the improved ResNeXt model still shows the optimal performance after sufficient comparison experiments with three other more advanced deep learning models. The experimental results show that the improved ResNeXt model is very suitable for the classification of early‐stage lung cancer serum Raman spectra and may provide a reference for future early screening studies of other cancers.