bluuebunny/crossref_metadata_2025_split huggingface dataset:
An examination of public support for 35 nutrition interventions across seven countries
Authors: Pettigrew Simone , Booth Leon , Dunford Elizabeth , Scapin Tailane , Webster Jacqui , Wu Jason , Tian Maoyi , Praveen D. , Sacks Gary 
Published: September 2022

Abstract
Background
Public support for evidence-based nutrition interventions can be an important determinant of government willingness to develop and implement such interventions. The aim of this study was to assess support for a broad range of nutrition interventions across seven countries: Australia, Canada, China, India, New Zealand, the United Kingdom, and the United States. Assessed interventions included those relating to food availability, affordability, reformulation, labelling, and promotion.

Methods
Approximately 1000 adults per country (total n = 7559) completed an online survey assessing support for 35 nutrition interventions/policies. ANOVA analyses were used to identify differences between countries on overall levels of support and by intervention category. Multiple regression analyses assessed demographic and diet-related factors associated with higher levels of support across the total sample and by country.

Results
Substantial levels of public support were found for the assessed interventions across the seven countries and five intervention categories. The highest levels were found in India (Mean across all interventions of 4.16 (standard deviation (SD) 0.65) on a 5-point scale) and the lowest in the United States (Mean = 3.48, SD = 0.83). Support was strongest for interventions involving food labelling (Mean = 4.20, SD = 0.79) and food reformulation (Mean = 4.17, SD = 0.87), and weakest for fiscal interventions (Mean = 3.52, SD = 1.06). Consumer characteristics associated with stronger support were higher self-rated health, higher educational attainment, female sex, older age, and perceptions of consuming a healthy diet.

Conclusion
The results indicate substantial support for a large range of nutrition interventions across the assessed countries, and hence governments could potentially be more proactive in developing and implementing such initiatives.
The ryanodine receptor mutational characteristics and its indication for cancer prognosis
Authors: Wang Fenglin , Yu Jingbo , Lin Ping , Sigalas Charalampos , Zhang Shibo , Gong Yuan , Sitsapesan Rebecca , Song Lele 
Published: September 2022

AbstractCa2+ signaling is altered substantially in many cancers. The ryanodine receptors (RYRs) are among the key ion channels in Ca2+ signaling. This study aimed to establish the mutational profile of RYR in cancers and investigate the correlation between RYR alterations and cancer phenotypes. The somatic mutation and clinical data of 11,000 cancer patients across 33 cancer types was downloaded from The Cancer Genome Atlas (TCGA) database. Subsequent data processing was performed with corresponding packages of the R software. Mutational profile was analyzed and its correlation with tumor mutational burden (TMB), patient prognosis, age and smoking status was analyzed and compared. All three RYR isoforms exhibited random mutational distribution without hotspot mutations when all cancers were analyzed together. The number of mutations in RYR2 (2388 mutations) far overweight that of RYR1 (1439 mutations) and RYR3 (1573 mutations). Linear correlation was observed between cumulative TMB and cumulative number of mutations for all RYR isoforms. Patients with RYR mutations exhibited significantly higher TMB than those without RYR mutations for most cancer types. Strong correlation was also revealed in the average number of mutations per person between pairs of RYR isoforms. No stratification of patient overall survival (OS) by mutational status was found for all three RYR isoforms when all cancers were analyzed together, however, significant stratification of OS by RYR mutations was revealed in several individual cancers, most strikingly in LUAD (P = 0.0067, RYR1), BLCA (P = 0.00071, RYR2), LUSC (P = 0.036, RYR2) and KIRC (P = 0.0042, RYR3). Furthermore, RYR mutations were correlated with higher age, higher smoking history grading and higher number of pack years. Characteristic mutation profile of RYRs in cancers has been revealed for the first time. RYR mutations were correlated with TMB, age, smoking status and capable of stratifying the prognosis of patients in several cancer types.
Bis-pharmacophore of cinnamaldehyde-clubbed thiosemicarbazones as potent carbonic anhydrase-II inhibitors
Authors: Rasool Asif , Batool Zahra , Khan Majid , Halim Sobia Ahsan , Shafiq Zahid , Temirak Ahmed , Salem Mohamed A. , Ali Tarik E. , Khan Ajmal , Al-Harrasi Ahmed 
Published: September 2022

AbstractHere, we report the synthesis, carbonic anhydrase-II (CA-II) inhibition and structure–activity relationship studies of cinnamaldehyde-clubbed thiosemicarbazones derivatives. The derivatives showed potent activities in the range of 10.3 ± 0.62–46.6 ± 0.62 µM. Among all the synthesized derivatives, compound 3n (IC50 = 10.3 ± 0.62 µM), 3g (IC50 = 12.1 ± 1.01 µM), and 3h (IC50 = 13.4 ± 0.52 µM) showed higher inhibitory activity as compared to the standard inhibitor, acetazolamide. Furthermore, molecular docking of all the active compounds was carried out to predict their behavior of molecular binding. The docking results indicate that the most active hit (3n) specifically mediate ionic interaction with the Zn ion in the active site of CA-II. Furthermore, the The199 and Thr200 support the binding of thiosemicarbazide moiety of 3n, while Gln 92 supports the interactions of all the compounds by hydrogen bonding. In addition to Gln92, few other residues including Asn62, Asn67, The199, and Thr200 play important role in the stabilization of these molecules in the active site by specifically providing H-bonds to the thiosemicarbazide moiety of compounds. The docking score of active hits are found in range of − 6.75 to − 4.42 kcal/mol, which indicates that the computational prediction correlates well with the in vitro results.
Reuse of phosphogypsum pretreated with water washing as aggregate for cemented backfill
Authors: Zhou Yanan , Li Xibing , Shi Ying , Zhu Quanqi , Du Jing 
Published: September 2022

AbstractPhosphogypsum (PG) is reused as aggregate in the cemented backfill, which effectively improves the PG reutilization efficiency. However, the massive impurities contained in aggregate PG would adversely affect the hydration of binder, and therefore deteriorate the strength development of backfill. This research starts with the feasibility study on pretreating PG with the water washing method. Based on the most economical principle of the water demand, the optimal conditions for washing PG were determined at a stirring time of 5 min and a solid–liquid ratio of 1:0.5. Then, the original and pretreated PG were made into the backfill. Compared to using the original PG, the backfill slurry using the pretreated PG had better fluidity performance, such as the lower slurry viscosity and the higher bleeding rate. Furthermore, with the pretreated aggregate PG, the backfill strength was significantly enhanced by more than 8 times. Finally, the environmental behavior of the cemented backfill was investigated. Using the pretreated PG as aggregate, concentrations of PO43− and F− in the bleeding water and backfill leachates could meet the Chinese standard for integrated wastewater discharge. The results extend the reuse of PG as aggregate in a more environmental-friendly way, meeting the needs for sustainable mines.
Pseudomonas aeruginosa persister cell formation upon antibiotic exposure in planktonic and biofilm state
Authors: Patel Hiral , Buchad Hasmatbanu , Gajjar Devarshi 
Published: September 2022

AbstractPersister cell (PC) is dormant, tolerant to antibiotics, and a transient reversible phenotype. These phenotypes are observed in P. aeruginosa and cause bacterial chronic infection as well as recurrence of biofilm-mediated infection. PC formation requires stringent response and toxin-antitoxin (TA) modules. This study shows the P. aeruginosa PC formation in planktonic and biofilm stages on ceftazidime, gentamicin, and ciprofloxacin treatments. The PC formation was studied using persister assay, flow cytometry using Redox Sensor Green, fluorescence as well as Confocal Laser Scanning Microscopy, and gene expression of stringent response and TA genes. In the planktonic stage, ceftazidime showed a high survival fraction, high redox activity, and elongation of cells was observed followed by ciprofloxacin and gentamicin treatment having redox activity and rod-shaped cells. The gene expression of stringent response and TA genes were upregulated on gentamicin followed by ceftazidime treatment and varied among the isolates. In the biofilm stage, gentamicin and ciprofloxacin showed the biphasic killing pattern, redox activity, gene expression level of stringent response and TA varied across the isolates. Ceftazidime treatment showed higher persister cells in planktonic growth while all three antibiotics were able to induce persister cell formation in the biofilm stage.
Care cascade of tuberculosis infection treatment for people living with HIV in the era of antiretroviral therapy scale-up
Authors: Lin Kuan-Yin , Yang Chia-Jui , Sun Hsin-Yun , Lee Yuan-Ti , Liou Bo-Huang , Hii Ing-Moi , Chen Tun-Chieh , Huang Sung-Hsi , Lee Chun-Yuan , Tsai Chin-Shiang , Lin Chi-Ying , Liu Chun-Eng , Chang Hsi-Yen , Cheng Chien-Yu , Lu Po-Liang , Hung Chien-Ching ,   Taiwan HIV Study Group, Huang Yu-Shan , Liu Wang-Da , Chen Guan-Jhou , Liu Wen-Chun , Su Yi-Ching , Wu Pei-Ying , Chen Ling-Ya , Zhang Jun-Yu , Tsai Mao-Song , Lin Chia-Chun , Lee Yu-Lin , Chen Yen-Hsu , Huang Yi-Chia , Ko Wen-Chien , Cheng Shu-Hsing , Chang Sui-Yuan , Wang Ning-Chi , Lin Te-Yu , Lee Yi-Chieh , Lin Shih-Ping , Hsieh Chia-Yin , Wang Hsiu-Wen , Ho Mao-Wang , Hung Tung-Che , Tsai Hung-Chin , Lin Hsi-Hsun , Lee Chen-Hsiang 
Published: September 2022

AbstractTesting and treatment of tuberculosis infection (TBI) are recommended for people living with HIV (PLWH). We aimed to evaluate the care cascade of TBI treatment among PLWH in the era of antiretroviral therapy (ART) scale-up. This retrospective study included adult PLWH undergoing interferon-gamma release assay (IGRA)-based TBI screening during 2019–2021. PLWH testing IGRA-positive were advised to receive directly-observed therapy for TBI after active TB disease was excluded. The care cascade was evaluated to identify barriers to TBI management. Among 7951 PLWH with a median age of 38 years and CD4 count of 616 cells/mm3, 420 (5.3%) tested positive and 38 (0.5%) indeterminate for IGRA. The TBI treatment initiation rate was 73.6% (309/420) and the completion rate was 91.9% (284/309). More than 80% of PLWH concurrently received short-course rifapentine-based regimens and integrase strand transfer inhibitor (InSTI)-containing ART. The main barrier to treatment initiation was physicians’ concerns and patients’ refusal (85.6%). The factors associated with treatment non-completion were older age, female, anti-HCV positivity, and higher plasma HIV RNA. Our observation of a high TBI completion rate among PLWH is mainly related to the introduction of short-course rifapentine-based regimens in the InSTI era, which can be the strategy to improve TBI treatment uptake.
Study on mechanical properties and fracture surface characteristics of argillaceous sandstone under different stress paths
Authors: Huang Tianzhu , Wang Lehua , Li Jianlin , Zhang Bingyi , Wang Xiaoping , Xu Xiaoliang 
Published: September 2022

AbstractTo study the differences in mechanical properties and failure characteristics of underground chambers surrounding rock under different stress conditions, triaxial loading and unloading tests were carried out on argillaceous sandstone. The three-dimensional topography parameters of the fracture surface were obtained by using high-precision three-dimensional topography scanning technology, including six height characteristic parameters and two texture parameters. Compared with the triaxial loading test, the strength, peak strain and residual strength of argillaceous sandstone with the same confining pressure under unloading conditions all decrease, and the stress–strain curve changes from ductility to brittleness. The Mogi–Coulomb strength criterion can better describe the strength properties of argillaceous sandstones than the Mohr–Coulomb and Drucker–Prager strength criteria. Under the unloading condition, the cohesion c decreased by 30.87% and the internal friction angle φ increased by 30.87% compared with the loading condition. The tensile cracks perpendicular to the unloading direction is formed during unloading, resulting in large roughness, dispersion and fluctuation of fracture surface.
Developing CIRdb as a catalog of natural genetic variation in the Canary Islanders
Authors: Díaz-de Usera Ana , Rubio-Rodríguez Luis A. , Muñoz-Barrera Adrián , Lorenzo-Salazar Jose M. , Guillen-Guio Beatriz , Jáspez David , Corrales Almudena , Íñigo-Campos Antonio , García-Olivares Víctor , Rodríguez Pérez María Del Cristo , Marcelino-Rodríguez Itahisa , Cabrera de León Antonio , González-Montelongo Rafaela , Flores Carlos 
Published: September 2022

AbstractThe current inhabitants of the Canary Islands have a unique genetic makeup in the European diversity landscape due to the existence of African footprints from recent admixture events, especially of North African components (> 20%). The underrepresentation of non-Europeans in genetic studies and the sizable North African ancestry, which is nearly absent from all existing catalogs of worldwide genetic diversity, justify the need to develop CIRdb, a population-specific reference catalog of natural genetic variation in the Canary Islanders. Based on array genotyping of the selected unrelated donors and comparisons against available datasets from European, sub-Saharan, and North African populations, we illustrate the intermediate genetic differentiation of Canary Islanders between Europeans and North Africans and the existence of within-population differences that are likely driven by genetic isolation. Here we describe the overall design and the methods that are being implemented to further develop CIRdb. This resource will help to strengthen the implementation of Precision Medicine in this population by contributing to increase the diversity in genetic studies. Among others, this will translate into improved ability to fine map disease genes and simplify the identification of causal variants and estimate the prevalence of unattended Mendelian diseases.
A study of green roof and impact on the temperature of buildings using integrated IoT system
Authors: Tseng Kuo-Hsiung , Chung Meng-Yun , Chen Li-Hsien , Chou Lu-An 
Published: September 2022

AbstractWith the rise of environmental consciousness and the evolution of circular cities, the Internet of things (IoT) has been combined with the concept of circular economies to promote the effective control of renewable energy and resources. In this study, a comprehensive IoT system containing front-end device applications, network layer innovations, and cloud platform integrations was used in civil engineering applications. This IoT architecture is presented as a development basis for constructing modular automatic monitoring devices and integrating circular city concepts with the IoT. According to the concept of circular city, green circulation and energy use are systematically integrated and called “green energy”. In addition, the green energy system can be divided into above-ground and underground. The above-ground part uses green roofs and solar panels for research and discussion. The composite solar green roofs of the two are called green roofs, and the comparison of their benefits is discussed on the spot. Narrowband IoT (NB-IoT) technologies were used in this study. The advantages of the developed system were analyzed using measured pH values, air temperatures, soil temperatures, and humidity. The results of this study indicate that constructing a green energy roof can decrease indoor temperatures by 1.5 °C and solar module temperatures by 1.6 °C while increasing power generation; thus, green energy roofs are suitable for tropical regions.
Micro-fragmented adipose tissue cellular composition varies by processing device and analytical method
Authors: Greenwood Valerie , Clausen Peter , Matuska Andrea M. 
Published: September 2022

AbstractAutologous adipose-derived biologics are of clinical interest based on accessibility of adipose tissue, a rich source of progenitor and immunomodulatory cells. Micro-fragmented adipose tissue (MFAT) preserves the cellular niche within intact extracellular matrix, potentially offering benefit over enzymatically-liberated stromal vascular fraction (SVF), however lack of standardized analyses complicate direct comparison of these products. In this study, MFAT from LipoGems® and AutoPose™ Restore systems, which utilize different washing and resizing methods, was analyzed for cellular content using different techniques. Flow cytometry was performed on SVF, with or without culture, and on the adherent cell population that naturally migrated from undigested MFAT. Cytokine release during culture was also assessed. SVF contained more diverse progenitor populations, while MFAT outgrowth contained lower cell concentrations of predominantly mesenchymal stromal cells (MSC). MSCs were significantly higher in MFAT from the AutoPose System for all analyses, with increased cytokine secretion characterized by high levels of anti-inflammatory and low to non-detectable inflammatory cytokines. These results demonstrate that cellularity depends on MFAT processing methods, and different techniques can be employed to evaluate graft cellularity. Comparisons of cell concentrations determined via these methods could be used to better interpret inter-study variability.
Identification and verification of CCNB1 as a potential prognostic biomarker by comprehensive analysis
Authors: Zeng Yinzhen , Fan Rong 
Published: September 2022

AbstractAs one of the most common types of pancreatic cancer, pancreatic ductal adenocarcinoma (PDAC) is highly invasive and lethal. This study aims to develop biomarkers and targets for the diagnosis and treatment of PDAC. Differentially expressed genes (DEGs) were screened via GEO2R, protein network was constructed through STRING and Cytoscape. Functional enrichment analysis was performed, followed by survival analysis and expression validation. A total of 115 DEGs were identified, including 108 upregulated and 7 downregulated genes. After enrichment, survival analysis, one potential gene, Cyclin B1 (CCNB1), was selected for further expression verification at the mRNA and protein level. Taker together, CCNB1 may act as a potential biomarker which provided new idea for elucidation of the pathogenesis of PDAC.
Silicon modifies C:N:P stoichiometry and improves the physiological efficiency and dry matter mass production of sorghum grown under nutritional sufficiency
Authors: de Carvalho Jonilson Santos , Frazão Joaquim José , de Mello Prado Renato , de Souza Júnior Jonas Pereira , Costa Milton Garcia 
Published: September 2022

AbstractSilicon (Si) may be involved in the modification of C:N:P stoichiometry and in physiological processes, increasing sorghum growth and grain production. The objective was to evaluate the effect of Si supply on C:N:P:Si stoichiometry, physiological response, growth, and grain production of sorghum. The experiment was carried out in pots with four concentrations of Si: 0; 1.2; 2.4; and 3.6 mmol L−1in a completely randomized design, with six replicates. Physiological attributes and dark green color index were measured and grain and biomass production were determined. Posteriorly, the plant material was ground to determine silicon (Si), carbon (C), nitrogen (N), and phosphorus (P) contents in order to analyze C:N:P:Si stoichiometry. C:Si and C:N ratios decreased at all Si concentrations applied (1.2, 2.4, and 3.6 mmol L−1) and in all plant parts studied, being lower at 3.6 mmol L−1. The lowest C:P ratios of leaves and roots were observed at 3.6 mmol L−1Si and the lowest C:P ratio of stems was observed at 1.2 mmol L−1Si. Si concentrations were not significant for the N:P ratio of leaves. The highest N:P ratio of stems was observed at 3.6 mmol L−1, while the lowest N:P ratio of roots was observed at 2.4 and 3.6 mmol L−1. Regardless of photosynthetic parameters, the application of 1.2 mmol L−1Si enhanced photosynthetic rate. The application of 2.4 and 3.6 mmol L−1enhanced stomatal conductance and dark green color index. The mass of 1000 grains was not influenced by Si applications, while Si applications at all concentrations studied (1.2, 2.4, and 3.6 mmol L−1) enhanced shoot and total dry matter, not affecting root dry matter and grain production. In conclusion, Si supply modifies C:N:P:Si stoichiometry and increases physiologic parameters, growth, development, and grain production in sorghum.
Sex differences in frailty of geriatric outpatients with type 2 diabetes mellitus: a multicentre cross-sectional study
Authors: Nguyen Huan Thanh , Nguyen An Huu , Le Phuong Thi My 
Published: September 2022

AbstractFrailty and type 2 diabetes mellitus (T2DM) can occur concurrently and are increasingly prevalent in older populations. There is a marked variability in frailty progression between men and women. This study aimed to investigate sex differences in the prevalence and factors associated with frailty in older outpatients with T2DM. This multicentre cross-sectional study included 638 outpatients (aged ≥ 60 years; median age 71 years [interquartile range, 66–77]; male, 55.5%) and was conducted from January 2019 to July 2020. Frailty was assessed using the Fried frailty phenotype. Factors associated with frailty were assessed using a logistic regression analysis. The overall frailty prevalence was 28.2% (men, 26.8%; women, 29.9%; P = 0.388). In the adjusted model, the factors associated with greater odds of being frail were older age (odds ratio [OR], 1.08; 95% confidence interval [CI], 1.05–1.11; P < 0.001) and body mass index (BMI) less than 20 kg/m2 (OR, 1.96; 95% CI, 1.16–3.32; P = 0.012). Higher education (OR, 0.64; 95% CI, 0.42–0.98; P = 0.041) and productive work (OR, 0.11; 95% CI, 0.03–0.36; P < 0.001) were protective factors against frailty. Frailty was associated with all four factors in women, but only with older age and productive work in men. Our study found that the prevalence of frailty in older outpatients with T2DM was 28.2%, though not significantly different between men and women. While older age and BMI less than 20 kg/m2 can increase the odds of frailty, and higher education and productive work can decrease the odds of frailty in women, only age and productive work were associated with frailty in men with T2DM.
Bioarchaeological investigations of the princely grave at Helmsdorf attesting to the violent death of an Early Bronze Age leader
Authors: Nicklisch Nicole , Ramsthaler Frank , Bunnefeld Jan-Heinrich , Schulz Georg , Friedrich Ronny , Alt Kurt W. , Meller Harald 
Published: September 2022

AbstractThe Helmsdorf “princely” tomb, excavated at the beginning of the twentieth century, is one of the most important archaeological discoveries dating from the Early Bronze Age in central Germany. In addition to the burial inventory, which points to an elevated social position of the deceased, a number of highly fragmented skeletal remains were preserved. Forensic anthropological investigation identified three distinctive bone defects, the surfaces of which were macromorphologically and microscopically examined in greater detail. Micro-CT analyses were also carried out. The results of all examinations suggested that the defects represented three perimortem injuries. The wound morphology was indicative of the use of a bladed weapon. The combination of injuries and their locations supported the assumption of a targeted use of force to kill. A comparison of Early Bronze Age weapons and tools with the bone lesions led to the identification of a type of weapon possibly used in the attack.