bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age and Injury Size Influence the Magnitude of Fecal Dysbiosis in Adult Burn Patients
Authors: Dyamenahalli Kiran , Choy Kevin , Frank Daniel N , Najarro Kevin , Boe Devin , Colborn Kathryn L , Idrovo Juan-Pablo , Wagner Anne L , Wiktor Arek J , Afshar Majid , Burnham Ellen L , McMahan Rachel H , Kovacs Elizabeth J 
Published: January 2022

Abstract
Clinical studies have demonstrated that age 50 years or older is an independent risk factor associated with poor prognosis after burn injury, the second leading cause of traumatic injuries in the aged population. While mechanisms driving age-dependent postburn mortality are perplexing, changes in the intestinal microbiome, may contribute to the heightened, dysregulated systemic response seen in aging burn patients. The fecal microbiome from 22 patients admitted to a verified burn center from July 2018 to February 2019 was stratified based on the age of 50 years and total burn surface area (TBSA) size of ≥10%. Significant differences (P = .014) in overall microbiota community composition (ie, beta diversity) were measured across the four patient groups: young &lt;10% TBSA, young ≥10% TBSA, older &lt;10% TBSA, and older ≥10% TBSA. Differences in beta diversity were driven by %TBSA (P = .013) and trended with age (P = .087). Alpha diversity components, richness, evenness, and Shannon diversity were measured. We observed significant differences in bacterial species evenness (P = .0023) and Shannon diversity (P = .0033) between the groups. There were significant correlations between individual bacterial species and levels of short-chain fatty acids. Specifically, levels of fecal butyrate correlated with the presence of Enterobacteriaceae, an opportunistic gut pathogen, when elevated in burn patients lead to worsen outcomes. Overall, our findings reveal that age-specific changes in the fecal microbiome following burn injuries may contribute to immune system dysregulation in patients with varying TBSA burns and potentially lead to worsened clinical outcomes with heightened morbidity and mortality.
miR-31, miR-155, and miR-221 Expression Profiles and Their Association With Graft Skin Tolerance in a Syngeneic vs Allogeneic Murine Skin Transplantation Model
Authors: Mandujano-Tinoco Edna Ayerim , González-García Francisco , Salgado Rosa M , Abarca-Buis René Fernando , Sanchez-Lopez José Manuel , Carranza-Castro Pilar Hazel , Padilla Luis , Krötzsch Edgar 
Published: January 2022

Abstract
Grafting is the preferred treatment for severe skin burns. Frequently, allogeneic tissue is the only transient option for wound coverage, but their use risks damage to surrounding tissues. MicroRNAs have been associated with acute rejection of different tissues/organs. In this study, we analyzed the expression of miR-31, miR-155, and miR-221 and associate it with graft tolerance or rejection using a murine full-thickness skin transplantation model. Recipient animals for the syngeneic and allogeneic groups were BALB/c and C57BL/6 mice, respectively; donor tissues were obtained from BALB/c mice. After 7 days posttransplantation (DPT), the recipient skin and grafts in the syngeneic group maintained most of their structural characteristics and transforming growth factor (TGF)-β1 and TGF-β3 expression. Allografts were rejected early (Banff grades II and IV at 3 and 7 DPT, respectively), showing damage to the skin architecture and alteration of TGF-β3 distribution. miRNAs skin expression changed in both mouse strains; miR-31 expression increased in the recipient skin of syngeneic grafts relative to that of allogeneic grafts at 3 and 7 DPT (P &lt; .05 and P &lt; .01, respectively); miR-221 expression increased in the same grafts at 7 DPT (P &lt; .05). The only significant difference between donor tissues was observed for miR-155 expression at 7 DPT which was associated with necrotic tissue. Only miR-31 and miR-221 levels were increased in the blood of BALB/c mice that received syngeneic grafts after 7 DPT. Our data suggest that local and systemic miR-31 and miR-221 overexpression are associated with graft tolerance.
Consumption of Supplemental Spring Protein Feeds by Western Honey Bee (Hymenoptera: Apidae) Colonies: Effects on Colony Growth and Pollination Potential
Authors: Hoover Shelley E , Ovinge Lynae P , Kearns Jeffery D 
Published: January 2022

Abstract
Adequate nutrition is required to support productive honey bee colonies, therefore beekeepers supplement colonies with additional protein at targeted time points. We tested the effects of commercially available protein feeds in spring, in advance of colonies being used for hybrid canola pollination. The feed treatments across the three-year study included the following patty types: Global 15% pollen, Global 0% pollen, Bee Pollen-Ate, FeedBee, and Healthy Bees, as well as an unsupplemented control in year two of the study only. The amount of feed consumed varied among colonies, treatments, date, and year. Similarly, there were also differences in feed efficiency (bees reared per gram of feed consumed), likely due to the relative availability of external forage sources to supplement the feed provided. Unsupplemented colonies were able to rear less brood, and subsequently had fewer adult bees than supplemented colonies, in an apiary where pollen was not abundant. Differences in consumption among treatments often failed to translate in to differences in amount of brood reared or subsequent adult population. All the protein feed treatments contained all ten amino acids essential to honey bees, however lysine and arginine were below the optimal proportion required for growth in all patties except the FeedBee patty. The amount of protein and amount and types of sugars and fats in the products also varied among product type and batch. The results of this study demonstrate a benefit to supplementary spring protein feeding to increase honey bee colony populations in advance of a summer pollination market.
Survey of malignant pleural mesothelioma treatment in Japan: Patterns of practice and clinical outcomes in tomotherapy facilities
Authors: Nakanishi-Imai Mikiko , Murai Taro , Onishi Masahiro , Mouri Atsuto , Komiyama Takafumi , Omura Motoko , Kudo Shigehiro , Miyamoto Akihiko , Hoshino Masaru , Ogawa Shinichi , Ohashi Shizuko , Koizumi Masahiko , Omagari Junichi , Mayahara Hiroshi , Karasawa Katsuyuki , Okumura Toshiyuki , Shibamoto Yuta 
Published: January 2022

Abstract
We conducted a nationwide survey of tomotherapy for malignant pleural mesothelioma (MPM) in Japan. Fifty-six facilities were surveyed and data on 31 patients treated curatively between 2008 and 2017 were collected from 14 facilities. Twenty patients received hemithorax irradiation after extrapleural pneumonectomy (EPP) (first group). Five patients received irradiation without EPP (second group), while six received salvage radiotherapy for local recurrence (salvage group). Among the seven patients not undergoing EPP, five (four in the second group and one in the salvage group) were treated with lung sparing pleural irradiation (LSPI) and two with irradiation to visible tumors. Two-year overall survival (OS) rates in the first and second groups were 33% and 60%, respectively (median, 13 vs 30 months, P = 0.82). In the first and second groups, 2-year local control (LC) rates were 53 and 67%, respectively (P = 0.54) and 2-year progression-free survival (PFS) rates were 16% and 60%, respectively (P = 0.07). Distant metastases occurred in 15 patients in the first group and three in the second group. In the salvage group, the median OS was 18 months. Recurrence was observed in the irradiated volume in four patients. The contralateral lung dose was higher in LSPI than in hemithorax irradiation plans (mean, 11.0 ± 2.2 vs 6.1 ± 3.1 Gy, P = 0.002). Grade 3 or 5 lung toxicity was observed in two patients receiving EPP and hemithorax irradiation, but not in those undergoing LSPI. In conclusion, outcomes of EPP and hemithorax irradiation were not satisfactory, whereas LSPI appeared promising and encouraging.
The Association Between Interpersonal Violence and Unstable Housing Among Veterans
Authors: Hargrave Anita S , Kimberg Leigh , Machtinger Edward L , Kushel Margot B , Cohen Beth E 
Published: January 2022

ABSTRACT

Background
Despite programs to address housing for Veterans, they continue to be at high risk of unstable housing. Interpersonal violence is also highly prevalent among Veterans and may contribute to unstable housing. Our study aimed to determine whether interpersonal violence was associated with unstable housing among Veterans, and how this association was influenced by common co-occurring conditions such as substance use and mental illness.

Methods
Veterans in the Mind Your Heart Study (N = 741) completed survey data on history of interpersonal violence and access to housing in the prior year. Interpersonal violence was defined as experiencing sexual violence, physical violence, or mugging/physical attack using the Brief Trauma Questionnaire. Multivariable models examined associations between interpersonal violence and unstable housing. Primary models were adjusted for age and sex. Potential explanatory factors were added in subsequent models, including marital status, education, income, substance use disorder, PTSD, and other mental illness.

Results
Veterans who had experienced interpersonal violence had almost twice the odds of unstable housing after adjustment for age and sex (AOR 1.9, 95% CI 1.2–3.0). This association was attenuated in the fully adjusted model including substance use, PTSD, and other mental illness, illustrating the interdependence of these factors (AOR 1.5, 95% CI 0.91–2.5). Subtypes of interpersonal violence were individually associated with increased odds of unstable housing after adjustment for age and sex (physical abuse AOR 1.7, 95% CI 1.2–2.5; mugging/physical attack AOR 1.8, 95% CI 1.2–2.7; sexual violence AOR 1.4, 95% CI 0.89–2.2), but were no longer significant in the fully adjusted model.

Conclusions
Previous experiences of interpersonal violence were associated with unstable housing among Veterans. Substance use, PTSD, and other mental illness played an important role in this relationship—highlighting the potential to improve health outcomes through trauma informed approaches that address mental health, substance use, and housing concurrently.
Testing modified (Horndeski) gravity by combining intrinsic galaxy alignments with cosmic shear
Authors: Reischke Robert , Bosca Victor , Tugendhat Tim , Schäfer Björn Malte 
Published: January 2022

ABSTRACT
We study the impact of modified gravity of the Horndeski class on intrinsic shape correlations in cosmic shear surveys. As these correlations intrinsic alignments are caused by tidal gravitational fields acting on galaxies as a collection of massive non-relativistic test particles, they are only sensitive to the gravitational potential, which forms in conjunction with the curvature perturbation. In contrast, the cosmic shear signal probes the sum of these two, i.e. both Bardeen potentials. Combining these probes therefore constitutes a test of gravity, derived from a single measurement. Focusing on linear scales and alignments of elliptical galaxies, we study the impact on inference of the braiding $\hat{\alpha }_{\rm B}$ and the time evolution of the Planck mass $\hat{\alpha }_{\rm M}$ by treating IAs as a genuine signal contributing to the overall ellipticity correlation. We find that for euclid, IAs can help to improve constraints on modified gravity of the Horndeski-class by 10 per cent if the alignment parameter needed for the linear alignment model is provided by simulations. If, however, the IA needs to be self-calibrated, all of the sensitivity is put into the inference of the alignment strength D since there is a very strong correlation with the evolution of the Planck mass. Thus, diminishing the benefit of IA for probing modified gravitational theories. While this paper shows results mainly for modified gravity parameters, similar deductions can be drawn for the investigation of anisotropic stresses, parametrized modifications to the Poisson equation, the phenomenology of gravitational slip and to breaking degeneracies in a standard cosmology.
Extremely massive disc galaxies in the nearby Universe form through gas-rich minor mergers
Authors: Jackson R A , Kaviraj S , Martin G , Devriendt J E G , Noakes-Kettel E A , Silk J , Ogle P , Dubois Y 
Published: January 2022

ABSTRACT
In our hierarchical structure-formation paradigm, the observed morphological evolution of massive galaxies – from rotationally supported discs to dispersion-dominated spheroids – is largely explained via galaxy merging. However, since mergers are likely to destroy discs, and the most massive galaxies have the richest merger histories, it is surprising that any discs exist at all at the highest stellar masses. Recent theoretical work by our group has used a cosmological, hydrodynamical simulation to suggest that extremely massive (M* &gt; 1011.4 M⊙) discs form primarily via minor mergers between spheroids and gas-rich satellites, which create new rotational stellar components and leave discs as remnants. Here, we use UV-optical and H i data of massive galaxies, from the Sloan Digital Sky Survey, Galaxy Evolution Explorer, Dark Energy Camera Legacy Survey (DECaLS), and Arecibo Legacy Fast ALFA surveys, to test these theoretical predictions. Observed massive discs account for ∼13 per cent of massive galaxies, in good agreement with theory (∼11 per cent). ∼64 per cent of the observed massive discs exhibit tidal features, which are likely to indicate recent minor mergers, in the deep DECaLS images (compared to ∼60 per cent in their simulated counterparts). The incidence of these features is at least four times higher than in low-mass discs, suggesting that, as predicted, minor mergers play a significant (and outsized) role in the formation of these systems. The empirical star formation rates agree well with theoretical predictions and, for a small galaxy sample with H i detections, the H i masses and fractions are consistent with the range predicted by the simulation. The good agreement between theory and observations indicates that extremely massive discs are indeed remnants of recent minor mergers between spheroids and gas-rich satellites.
Mechanisms of Cre recombinase synaptic complex assembly and activation illuminated by Cryo-EM
Authors: Stachowski Kye , Norris Andrew S , Potter Devante , Wysocki Vicki H , Foster Mark P 
Published: January 2022

Abstract
Cre recombinase selectively recognizes DNA and prevents non-specific DNA cleavage through an orchestrated series of assembly intermediates. Cre recombines two loxP DNA sequences featuring a pair of palindromic recombinase binding elements and an asymmetric spacer region, by assembly of a tetrameric synaptic complex, cleavage of an opposing pair of strands, and formation of a Holliday junction intermediate. We used Cre and loxP variants to isolate the monomeric Cre-loxP (54 kDa), dimeric Cre2-loxP (110 kDa), and tetrameric Cre4-loxP2 assembly intermediates, and determined their structures using cryo-EM to resolutions of 3.9, 4.5 and 3.2 Å, respectively. Progressive and asymmetric bending of the spacer region along the assembly pathway enables formation of increasingly intimate interfaces between Cre protomers and illuminates the structural bases of biased loxP strand cleavage order and half-the-sites activity. Application of 3D variability analysis to the tetramer data reveals constrained conformational sampling along the pathway between protomer activation and Holliday junction isomerization. These findings underscore the importance of protein and DNA flexibility in Cre-mediated site selection, controlled activation of alternating protomers, the basis for biased strand cleavage order, and recombination efficiency. Such considerations may advance development of site-specific recombinases for use in gene editing applications.
Monoclonal Antibody Therapy for COVID-19: A Public Health Perspective From Arkansas
Authors: Kothari Atul , Woodland Borella Elizabeth , Smith Michelle R 
Published: January 2022

Abstract
COVID-19 monoclonal antibodies revolutionized the treatment for eligible patients who have tested positive for SARS CoV-2 infection in an ambulatory setting. In this short report, we describe our experience assisting in the distribution of monoclonal antibodies in Arkansas during the summer surge of the delta variant.
A DE1 BINDING FACTOR 1–GLABRA2 module regulates rhamnogalacturonan I biosynthesis in Arabidopsis seed coat mucilage
Authors: Xu Yan , Wang Yiping , Du Jinge , Pei Shengqiang , Guo Shuaiqiang , Hao Ruili , Wang Dian , Zhou Gongke , Li Shengjun , O’Neill Malcolm , Hu Ruibo , Kong Yingzhen 
Published: January 2022

Abstract
The mucilage surrounding hydrated Arabidopsis thaliana seeds is a specialized extracellular matrix composed mainly of the pectic polysaccharide rhamnogalacturonan I (RG-I). Although, several genes responsible for RG-I biosynthesis have been identified, the transcriptional regulatory mechanisms controlling RG-I production remain largely unknown. Here we report that the trihelix transcription factor DE1 BINDING FACTOR 1 (DF1) is a key regulator of mucilage RG-I biosynthesis. RG-I biosynthesis is significantly reduced in loss-of-function mutants of DF1. DF1 physically interacts with GLABRA2 (GL2) and both proteins transcriptionally regulate the expression of the RG-I biosynthesis genes MUCILAGE MODIFIED 4 (MUM4) and GALACTURONOSYLTRANSFERASE-LIKE5 (GATL5). Through chromatin immunoprecipitation-quantitative PCR and transcriptional activation assays, we uncover a cooperative mechanism of the DF1–GL2 module in activating MUM4 and GATL5 expression, in which DF1 binds to the promoters of MUM4 and GATL5 through interacting with GL2 and facilitates the transcriptional activity of GL2. The expression of DF1 and GL2 is directly regulated by TRANSPARENT TESTA GLABRA2 (TTG2) and, in turn, DF1 directly represses the expression of TTG2. Taken together, our data reveal that the transcriptional regulation of mucilage RG-I biosynthesis involves a regulatory module, comprising DF1, GL2, and TTG2.
From crisis to reform? Exploring three post-COVID pathways
Authors: Boin Arjen , ‘t Hart Paul 
Published: January 2022

Abstract
Crises are often viewed as catalysts for change. The coronavirus disease crisis is no exception. In many policy sectors, proponents of reform see this global crisis both as a justification and an enabler of necessary change. Policy scholars have paid ample attention to this crisis-reform thesis. Empirical research suggests that these proponents of crisis-induced change should not be too optimistic. The question remains why some crises give rise to reform whereas so many others do not. This paper focuses on one particular factor that crisis researchers have identified as important. Crisis research suggests that the outcome of the meaning-making process—the efforts to impose a dominant frame on a population—shapes the prospects of postcrisis change. The paper offers three ideal-typical framing scripts, which researchers can use to study postcrisis trajectories.
What has happened and what has not happened due to the coronavirus disease pandemic: a systemic perspective on policy change
Authors: Knill Christoph , Steinebach Yves 
Published: January 2022

Abstract
The societal and policy transformations associated with the coronavirus disease pandemic are currently subject of intense academic debate. In this paper, we contribute to this debate by adopting a systemic perspective on policy change, shedding light on the hidden and indirect crisis effects. Based on a comprehensive analysis of policy agenda developments in Germany, we find that the pandemic led to profound shifts in political attention across policy areas. We demonstrate that these agenda gains and losses per policy area vary by the extent to which the respective areas can be presented as relevant in managing the coronavirus disease crisis and its repercussions. Moreover, relying on the analysis of past four economic crises, we also find that there is limited potential for catching up dynamics after the crisis is over. Policy areas that lost agenda share during crisis are unlikely to make up for these losses by strong attention gains once the crisis is over. Crises have hence substantial, long-term and so far, neglected effects on policymaking in modern democracies.
Re-thinking the coronavirus pandemic as a policy punctuation: COVID-19 as a path-clearing policy accelerator
Authors: Hogan John , Howlett Michael , Murphy Mary 
Published: January 2022

AbstractThis article joins with others in this special issue to examine the evolution of our understanding of how the coronavirus disease (COVID)-19 pandemic impacted policy ideas and routines across a wide variety of sectors of government activity. Did policy ideas and routines transform as a result of the pandemic or were they merely a continuation of the status quo ante? If they did transform, are the transformations temporary in nature or likely to lead to significant, deep and permanent reform to existing policy paths and trajectories? As this article sets out, the literature on policy punctuations has evolved and helps us understand the impact of COVID-19 on policy-making but tends to conflate several distinct aspects of path trajectories and deviations under the general concept of “critical junctures” which muddy reflections and findings. Once the different possible types of punctuations have been clarified, however, the result is a set of concepts related to path creation and disruption—especially that of “path clearing”—which are better able to provide an explanation of the kinds of policy change to be expected to result from the impact of events such as the 2019 coronavirus pandemic.
Policy integration, problem-solving, and the coronavirus disease crisis: lessons for policy design
Authors: Maggetti Martino , Trein Philipp 
Published: January 2022

AbstractThe coronavirus disease pandemic has exposed differences in the capacity of governments around the world to integrate and coordinate different policy instruments into a coherent response. In this article, we conceptualize and empirically examine policy integration in responses to the coronavirus disease crisis in 35 countries. We then discuss how the interplay between restrictions, health protection, and economic policy has been articulated between, on the one hand, a policy design based on the complementarity of pro-public health and pro-economy measures, implying an integrated response, and, on the other, a policy design based on the perception of an inherent trade-off between the two. Finally, we discuss three implications from our analysis of policy integration against the coronavirus disease crisis for the post-COVID state: (a) the normalization and adaptation of integrated crisis responses; (b) the possible acceleration and “catching up” of problem-solving capacity as governments may use the crisis as an instance to put into place new social policies; and (c) policy integration as an accelerator of policy complexity and resistance against technocracy in the post-COVID state.