bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGARUH DAYA TARIK WISATA, AKSESIBILITAS DAN FASILITAS TERHADAP KEPUTUSAN BERKUNJUNG WISATAWAN KE PANTAI SIANGAU KABUPATEN BANGKA BARAT
Authors: Ardiansyah Koko , Sumar  , Nugroho Ari Agung 
Published: April 2022

The relatively low number of tourists in Siangau Beach motivated the present writer to analyze the effects of Attractions, Accessibility and Facilities on Touristâ€™s Decisions to Visit Siangau Beach in West Bangka Regency. This research uses quantitative approach with a total sample of 95 respondents, who were seleted using non-probability sampling. The independent variables in this research are attraction, accessibility and facilities, while the dependent variable is decision to visit. The data we analyzed using multiple linear regression with t-test, F test and R2. The multiple regression analysis model obtained was Y= -3,209 + 0,605+ 0,580Â + 0,426; The research results indicate that partially, tourist attraction, accessibility and facilities have positive and significant effect on touristsâ€™ decisions to visit Saingau Beach in West Bangka Regency. Meanwhile the F-test results indicates that attraction, accessibility and facilities simultaneously have positive and significant effect on touristsâ€™ decision to visit Siangau Beach in West Bangka Regency. Coefficient of determination (R2) test results indicates that the research model can explain 58,6% of touristsâ€™ decisions to visit Siangau Beach, while 41,4% was affected by other variables outside of the research, wich can be further investigated..Â Keywords: Attraction, Accessibility, Facilities, Decision to Visit
Penerapan E-Filing Dalam Meningkatkan Kesadaran Hukum Wajib Pajak Orang Pribadi (Studi Kantor Pelayanan Pajak Pratama Madiun)
Authors: Pradhana Angga pramodya , Subadi  , Taufiq Yuli Purnama  , Zulfa Rahima Cahyasari  
Published: April 2022

Abstract--The purpose of this study is to determine the application of e-filing as a means of increasing legal awareness for taxpayers, especially individuals and to find out the supporting factors and obstacles in implementing e-filing for the Madiun area. In this study, the researcher used empirical methods, namely data obtained directly through field research with primary data sources, secondary data sources and tertiary data sources and data collection techniques carried out by observation, interviews, document studies to informants using qualitative descriptive data analysis. The results of this study indicate that the implementation of e-filing at the Pelayanan Pajak Pratama (KPPP) Madiun Office is going well and has increased almost every year, with many benefits that make it easier for KPP and taxpayers, especially during this pandemic, although there are some shortcomings, especially in terms of socialization about e-filing and the down system that occurs in its implementation. However, the other stages and contents have been carried out well, especially in the implementation of e-filing. There are 2 (two) benefits in this research, namely practical and theoretical, practically to develop legal knowledge and add references for readers, theoretically in KPPP Madiun can be used as input in order to increase the application of e-filing in mandatory legal awareness taxes to be a good and practical solution in reporting the Annual SPT.
Keywords: e-filing, Taxpayer, Kantor Pelayanan Pajak Pratama Madiun.
Rapid Manufacturing of Highly Cytotoxic Clinical-Grade SARS-CoV-2-specific T Cell Products Covering SARS-CoV-2 and Its Variants for Adoptive T Cell Therapy
Authors: Bonifacius Agnes , Tischer-Zimmermann Sabine , Santamorena Maria Michela , Mausberg Philip , Schenk Josephine , Koch Stephanie , Barnstorf-Brandes Johanna , Gödecke Nina , Martens Jörg , Goudeva Lilia , Verboom Murielle , Wittig Jana , Maecker-Kolhoff Britta , Baurmann Herrad , Clark Caren , Brauns Olaf , Simon Martina , Lang Peter , Cornely Oliver A. , Hallek Michael , Blasczyk Rainer , Seiferling Dominic , Köhler Philipp , Eiz-Vesper Britta 
Published: April 2022

Objectives: Evaluation of the feasibility of SARS-CoV-2-specific T cell manufacturing for adoptive T cell transfer in COVID-19 patients at risk to develop severe disease.Methods: Antiviral SARS-CoV-2-specific T cells were detected in blood of convalescent COVID-19 patients following stimulation with PepTivator SARS-CoV-2 Select using Interferon-gamma Enzyme-Linked Immunospot (IFN-γ ELISpot), SARS-CoV-2 T Cell Analysis Kit (Whole Blood) and Cytokine Secretion Assay (CSA) and were characterized with respect to memory phenotype, activation state and cytotoxic potential by multicolor flow cytometry, quantitative real-time PCR and multiplex analyses. Clinical-grade SARS-CoV-2-specific T cell products were generated by stimulation with MACS GMP PepTivator SARS-CoV-2 Select using CliniMACS Prodigy and CliniMACS Cytokine Capture System (IFN-gamma) (CCS). Functionality of enriched T cells was investigated in cytotoxicity assays and by multiplex analysis of secreted cytotoxic molecules upon target recognition.Results: Donor screening via IFN-γ ELISpot allows for pre-selection of potential donors for generation of SARS-CoV-2-specific T cells. Antiviral T cells reactive against PepTivator SARS-CoV-2 Select could be magnetically enriched from peripheral blood of convalescent COVID-19 patients by small-scale CSA resembling the clinical-grade CCS manufacturing process and showed an activated and cytotoxic T cell phenotype. Four clinical-grade SARS-CoV-2-specific T cell products were successfully generated with sufficient cell numbers and purities comparable to those observed in donor pretesting via CSA. The T cells in the generated products were shown to be capable to replicate, specifically recognize and kill target cells in vitro and secrete cytotoxic molecules upon target recognition. Cell viability, total CD3+ cell number, proliferative capacity and cytotoxic potential remained stable throughout storage of up to 72 h after end of leukapheresis.Conclusion: Clinical-grade SARS-CoV-2-specific T cells are functional, have proliferative capacity and target-specific cytotoxic potential. Their function and phenotype remain stable for several days after enrichment. The adoptive transfer of partially matched, viable human SARS-CoV-2-specific T lymphocytes collected from convalescent individuals may provide the opportunity to support the immune system of COVID-19 patients at risk for severe disease.
Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes
Authors: Loginov Sergey V. , Fermie Job , Fokkema Jantina , Agronskaia Alexandra V. , De Heus Cilia , Blab Gerhard A. , Klumperman Judith , Gerritsen Hans C. , Liv Nalan 
Published: April 2022

Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology. Volume or three-dimensional (3D) correlative light and electron microscopy (volume-CLEM) holds unique potential to explore cellular physiology at high-resolution ultrastructural detail across cell volumes. However, the application of volume-CLEM is hampered by limitations in throughput and 3D correlation efficiency. In order to address these limitations, we describe a novel pipeline for volume-CLEM that provides high-precision (&lt;100 nm) registration between 3D fluorescence microscopy (FM) and 3D electron microscopy (EM) datasets with significantly increased throughput. Using multi-modal fiducial nanoparticles that remain fluorescent in epoxy resins and a 3D confocal fluorescence microscope integrated into a Focused Ion Beam Scanning Electron Microscope (FIB.SEM), our approach uses FM to target extremely small volumes of even single organelles for imaging in volume EM and obviates the need for post-correlation of big 3D datasets. We extend our targeted volume-CLEM approach to include live-cell imaging, adding information on the motility of intracellular membranes selected for volume-CLEM. We demonstrate the power of our approach by targeted imaging of rare and transient contact sites between the endoplasmic reticulum (ER) and lysosomes within hours rather than days. Our data suggest that extensive ER-lysosome and mitochondria-lysosome interactions restrict lysosome motility, highlighting the unique capabilities of our integrated CLEM pipeline for linking molecular dynamic data to high-resolution ultrastructural detail in 3D.
Aberrant Expression of Cardiac Troponin-T in Lung Cancer Tissues in Association With Pathological Severity
Authors: Tsuruda Toshihiro , Sato Yuichiro , Tomita Masaki , Tanaka Hiroyuki , Hatakeyama Kinta , Otsu Misa , Kawano Aya , Nagatomo Keiko , Yoshikawa Naoki , Ikeda Ryuji , Asada Yujiro , Kaikita Koichi 
Published: April 2022

BackgroundCardiac troponin-T (TNNT2) is exclusively present in cardiac muscle. Measurement of TNNT2 is used for diagnosing acute coronary syndrome. However, its expression may not be limited in myocardium. This study aimed at evaluating the expression of TNNT2 in neoplastic tissues.Methods and ResultsWe used paraffin-embedded blocks of 68 patients with lung cancer (age, 68 ± 11 years old; early-stage, 33; advance-stage, 35) at Miyazaki University Hospital, Japan between January 1, 2017, and March 31, 2019. We stained the slide sections with primary monoclonal antibody against TNNT2 protein, and assessed the frequency of positive staining, and its association with pathological severity. In addition, we examined whether TNNT2 gene is detected in lung cancer tissues of four patients using reverse transcription-polymerase chain reaction. Immunoreactivity for TNNT2 protein was present in the cytoplasm and nucleus of lung cancer cells. The frequency was 37% (25 of 68) in all patients and was irrespective of histologic type (six of 13, squamous cell carcinoma; 18 of 50, adenocarcinoma; 0 of 4, neuroendocrine cell carcinoma; 1 of 1, large cell carcinoma). The prevalence increased with pathological staging [9% (3 of 33) at early-stage (Stage 0–I); 63% (22 of 35) at advance-stage (Stage II–IV and recurrence)]. In addition, frequency of positive staining for TNNT2 increased with pleural (χ2 = 5.877, P = 0.015) and vascular (χ2 = 2.449, P = 0.118) invasions but decreased with lymphatic invasion (χ2 = 3.288, P = 0.070) in specimens performed surgical resection. Furthermore, TNNT2 mRNA was detected in the resected squamous cell carcinoma and adenocarcinoma tissues.ConclusionsOur data suggest the aberrant expression of TNNT2 in lung cancer and its prevalence increases with pathological severity.
Geochemical and Geological Characterization of Upper Permian Linghao Formation Shale in Nanpanjiang Basin, SW China
Authors: Gu Yifan , Cai Guangyin , Hu Dongfeng , Wei Zhihong , Liu Ruobing , Han Jing , Fan Zhiwei , Hao Jingyu , Jiang Yuqiang 
Published: April 2022

The Upper Permian Linghao Formation shale is the most potential shale gas exploration target in Nanpanjiang Basin. In this study, X-ray diffraction, field emission scanning electron microscopy, CH4 isothermal adsorption, and nuclear magnetic resonance cryoporometry are intergrated to reveal comprehensive characterization of Linghao Formation shale collected from a well in Nanpanjiang Basin. Results indicate that organic-rich shales developed in the Ling 1 member and the lower part of Ling 3 member. The organic-rich shales are predominantly characterized by kerogen type I, with a relatively highly mature to overmature status. The Ling 1 organic-rich shale mainly consists of mixed shale lithofacies, and the organic-rich shale in the lower part of Ling 3 is mainly composed of argillaceous shale. The pore volume in Ling 1 organic-rich shale is mainly contributed by 3- to 6-nm and 8- to 11-nm organic pores. The pore volume of Ling 3 organic-rich shale is mainly contributed by 2- to 3-nm and 4- to 11-nm organic pores. The organic pores between 3 and 10 nm also have a small contribution to the pore volume. The absolute adsorption gas content of Ling 1 and Ling 3 organic-rich shale is 1.21 m3/t and 1.64 m3/t, respectively. The absolute adsorption gas content of Ling 1 and Ling 3 organic-rich shale exceeds the minimum standard for commercial shale gas development in China (1.0 m3/t). According to the adsorption gas ratio of 50%, the total gas content of Ling 1 and Ling 3 organic-rich shale can reach 3.28 m3/t and 2.28 m3/t, respectively. It is suggested that the Upper Permian Linghao Formation shale in the Nanpanjiang Basin has a significant potential for shale gas exploration.
The Recent Relationships Between Andean Ice-Core Dust Record and Madeira River Suspended Sediments on the Wet Season
Authors: Reis Rafael S. dos , da Rocha Ribeiro Rafael , Delmonte Barbara , Ramirez Edson , Dani Norberto , Mayewski Paul A. , Simões Jefferson C. 
Published: April 2022

Dust particle studies in ice cores from the tropical Andes provide important information about climate dynamics. We investigated dust concentrations from a 22.7 m ice-core recovered from the Quelccaya Ice Cap (QIC) in 2018, representing 14 years of snow accumulation. The dust seasonality signal was still preserved with homogenization of the record due to surface melting and percolation. Using a microparticle counter, we measured the dust concentration from 2 to 60 µm and divided the annual dust concentration into three distinct groups: fine particle percentage (FPP, 2–10 µm), coarse particle percentage (CPP, 10–20 μm), and giant particle percentage (GPP, 20–60 μm). Increased dust was associated with the warm stage of the Pacific Decadal Oscillation index (PDO) after 2013 with significant increases in FPP and a relative decrease in CPP and GPP. There was a positive correlation between PDO and FPP (r = 0.70, p-value &lt; 0.005). CPP and GPP were dominant during the mainly PDO cold phase (2003–2012). The FPP increase record occurs during the positive phase of PDO and snow accumulation decrease. We also revealed a potential link between QIC record and Madeira River during the wet season through two relationships: between QIC snow accumulation and runoff during transitional season, QIC dust, and suspended sediments during high-water discharge. The snow accumulation (during September-November) and runoff (during November-January) relationship present similar variability using a time-lag (60 days) while total dust and FPP group are associated with average suspended sediments concentration during February-April. Assessing dust record variability by distinct size groups can help to improve our knowledge of how the Pacific ocean influence dust record in the QIC. In addition, the association of snow accumulation and dust variability with dynamic changes in suspended sediments load and runoff in the Madeira River system demonstrates the potential for future investigation of linkages between QIC record and Amazon basin rivers.
Sex-Specific Differences in MicroRNA Expression During Human Fetal Lung Development
Authors: Lin Nancy W. , Liu Cuining , Yang Ivana V. , Maier Lisa A. , DeMeo Dawn L. , Wood Cheyret , Ye Shuyu , Cruse Margaret H. , Smith Vong L. , Vyhlidal Carrie A. , Kechris Katerina , Sharma Sunita 
Published: April 2022

Background: Sex-specific differences in fetal lung maturation have been well described; however, little is known about the sex-specific differences in microRNA (miRNA) expression during human fetal lung development. Interestingly, many adult chronic lung diseases also demonstrate sex-specific differences in prevalence. The developmental origins of health and disease hypothesis suggests that these sex-specific differences in fetal lung development may influence disease susceptibility later in life. In this study, we performed miRNA sequencing on human fetal lung tissue samples to investigate differential expression of miRNAs between males and females in the pseudoglandular stage of lung development. We hypothesized that differences in miRNA expression are present between sexes in early human lung development and may contribute to the sex-specific differences seen in pulmonary diseases later in life.Methods: RNA was isolated from human fetal lung tissue samples for miRNA sequencing. The count of each miRNA was modeled by sex using negative binomial regression models in DESeq2, adjusting for post-conception age, age2, smoke exposure, batch, and RUV factors. We tested for differential expression of miRNAs by sex, and for the presence of sex-by-age interactions to determine if miRNA expression levels by age were distinct between males and females.Results: miRNA expression profiles were generated on 298 samples (166 males and 132 females). Of the 809 miRNAs expressed in human fetal lung tissue during the pseudoglandular stage of lung development, we identified 93 autosomal miRNAs that were significantly differentially expressed by sex and 129 miRNAs with a sex-specific pattern of miRNA expression across the course of the pseudoglandular period.Conclusion: Our study demonstrates differential expression of numerous autosomal miRNAs between the male and female developing human lung. Additionally, the expression of some miRNAs are modified by age across the pseudoglandular stage in a sex-specific way. Some of these differences in miRNA expression may impact susceptibility to pulmonary disease later in life. Our results suggest that sex-specific miRNA expression during human lung development may be a potential mechanism to explain sex-specific differences in lung development and may impact subsequent disease susceptibility.
A Novel Inflammatory Response–Related Gene Signature Improves High-Risk Survival Prediction in Patients With Head and Neck Squamous Cell Carcinoma
Authors: Han Yanxun , Ding Zhao , Chen Bangjie , Liu Yuchen , Liu Yehai 
Published: April 2022

Background: Head and neck squamous cell carcinoma (HNSCC) is a highly prevalent and malignant tumor that is difficult to effectively prognosticate outcomes. Recent reports have suggested that inflammation is strongly related to tumor progression, and several biomarkers linked to inflammation have been demonstrated to be useful for making a prognosis. The goal of this research was to explore the relevance between the inflammatory-related genes and HNSCC prognosis.Methods: The clinical information and gene expression data of patients with HNSCC were acquired from publicly available data sources. A multigene prognostic signature model was constructed in The Cancer Genome Atlas and verified in the Gene Expression Omnibus database. According to the risk score calculated for each patient, they were divided into low- and high-risk groups based on the median. The Kaplan–Meier survival curve and receiver operating characteristic curve were applied to determine the prognostic value of the risk model. Further analysis identified the independent prognostic factors, and a prognostic nomogram was built. The relationship between tumor immune infiltration status and risk scores was investigated using Spearman correlation analysis. Finally, to confirm the expression of genes in HNSCC, quantitative real-time polymerase chain reaction (qRT-PCR) was performed.Results: A prognostic model consisting of 14 inflammatory-related genes was constructed. The samples with a high risk had an apparently shorter overall survival than those with a low risk. Independent prognostic analysis found that risk scores were a separate prognostic factor in HNSCC patients. Immune infiltration analysis suggested that the abundance of B cells, CD8 T cells, M2 macrophages, myeloid dendritic cells, and monocytes in the low-risk group was higher, while that of M0, M1 macrophages, and resting NK cells was obviously higher in the high-risk group. The risk scores were related to chemotherapeutic sensitivity and the expression of several immune checkpoint genes. Moreover, CCL22 and IL10 were significantly higher in HNSCC tissues, as determined by qRT-PCR.Conclusion: Taken together, we constructed a novel inflammatory response–related gene signature, which may be used to estimate outcomes for patients with HNSCC and may be developed into a powerful tool for forecasting the efficacy of immunotherapeutic and chemotherapeutic drugs for HNSCC.
Identification of Hub Genes in Idiopathic Pulmonary Fibrosis and NSCLC Progression:Evidence From Bioinformatics Analysis
Authors: Yao Yuanshan , Li Zheng , Gao Wen 
Published: April 2022

Background: Lung cancer is the most common comorbidity of idiopathic pulmonary fibrosis. Thus there is an urgent need for the research of IPF and carcinogenesisObjective: The objective of this study was to explore hub genes which are common in pulmonary fibrosis and lung cancer progression through bioinformatic analysis.Methods: All the analysis was performed in R software. Differentially expressed genes (DEGs) were explored by comparing gene expression profiles between IPF tissues and healthy lung tissues from GSE24206, GSE53845, GSE101286 and GSE110147 datasets. Venn Diagram analysis was used to identify the overlapping genes, while GO and KEGG pathway enrichment analysis were used to explore the biological functions of the DEGs using clusterprofiler package. Hub genes were identified by analyzing protein-protein interaction networks using Cytoscape software. Nomogram was constructed using the rms package. Tumor immune dysfunction and exclusion (TIDE) and Genomics of Drug Sensitivity in Cancer (GDSC) analysis was used to quantify the immunotherapy and chemotherapy sensitivity of non-small cell lung cancer (NSCLC) patients.Results:COL1A1, COL3A1, MMP1, POSTN1 and TIMP3 were identified as the top five hub genes. The five hub genes were used to construct a diagnostic nomogram that was validated in another IPF dataset. Since the hub genes were also associated with lung cancer progression, we found that the nomogram also had diagnostic value in NSCLC patients. These five genes achieved a statistically difference of overall survival in NSCLC patients (p &lt; 0.05). The expression of the five hub genes was mostly enriched in fibroblasts. Fibroblasts and the hub genes also showed significant ability to predict the susceptibility of NSCLC patients to chemotherapy and immunotherapy.Conclusion: We identified five hub genes as potential biomarkers of IPF and NSCLC progression. This finding may give insight into the underlying molecular mechanisms of IPF and lung cancer progression and provides potential targets for developing new therapeutic agents for IPF patients.