bluuebunny/crossref_metadata_2025_split huggingface dataset:
miRNA‐encapsulated abiotic materials and biovectors for cutaneous and oral wound healing: Biogenesis, mechanisms, and delivery nanocarriers
Authors: Deka Dey Asmita , Yousefiasl Satar , Kumar Arun , Dabbagh Moghaddam Farnaz , Rahimmanesh Ilnaz , Samandari Mohamadmahdi , Jamwal Sumit , Maleki Aziz , Mohammadi Abbas , Rabiee Navid , Cláudia Paiva‐Santos Ana , Tamayol Ali , Sharifi Esmaeel , Makvandi Pooyan 
Published: May 2022

AbstractMicroRNAs (miRNAs) as therapeutic agents have attracted increasing interest in the past decade owing to their significant effectiveness in treating a wide array of ailments. These polymerases II‐derived noncoding RNAs act through post‐transcriptional controlling of different proteins and their allied pathways. Like other areas of medicine, researchers have utilized miRNAs for managing acute and chronic wounds. The increase in the number of patients suffering from either under‐healing or over‐healing wound demonstrates the limited efficacy of the current wound healing strategies and dictates the demands for simpler approaches with greater efficacy. Various miRNA can be designed to induce pathway beneficial for wound healing. However, the proper design of miRNA and its delivery system for wound healing applications are still challenging due to their limited stability and intracellular delivery. Therefore, new miRNAs are required to be identified and their delivery strategy needs to be optimized. In this review, we discuss the diverse roles of miRNAs in various stages of wound healing and provide an insight on the most recent findings in the nanotechnology and biomaterials field, which might offer opportunities for the development of new strategies for this chronic condition. We also highlight the advances in biomaterials and delivery systems, emphasizing their challenges and resolutions for miRNA‐based wound healing. We further review various biovectors (e.g., adenovirus and lentivirus) and abiotic materials such as organic and inorganic nanomaterials, along with dendrimers and scaffolds, as the delivery systems for miRNA‐based wound healing. Finally, challenges and opportunities for translation of miRNA‐based strategies into clinical applications are discussed.
Immediate implant and socket preservation using sticky bone and leukocyte‐platelet‐rich fibrin in the anterior maxilla: A 3‐year case report
Authors: Cirmeni Marco , Fedele Ottavio , Giammarinaro Enrica , Marconcini Simone , Covani Ugo , Caso Guerino 
Published: May 2022

AbstractIntroductionImmediate implant in the esthetic area is a challenging procedure. Chronic infection often contributes to extensive bone loss and acute infection often precludes a safe, clean surgery. However, it is quite common to come across failing teeth in the esthetic area, where the patient often demands a faster rehabilitation.Case PresentationIn this clinical case, a fractured upper central incisor was replaced by an immediate implant, and the perimetrical area was filled with heterologous bone blended with patient‐derived leukocyte‐ and platelet‐rich fibrin (L‐PRF) in the form of sticky bone. A Maryland bridge was bonded to the adjacent teeth with the intent to guarantee minimum esthetic to the patient. After 5 months, the implant was connected to a definitive abutment and crown. The facial and interdental soft tissue was maintained with appreciable success after 2 years. The pre‐treatment and 2‐year‐ post‐treatment computed tomography scans revealed marginal bone preservation.ConclusionThe use of sticky bone and L‐PRF in immediate implant sites helps prevent hard and soft‐tissue collapse and may favor faster and sounder healing.Key pointsWhy is this case new information?

It provides support to the efficacy of immediate implant placement with simultaneous use of L‐PRF.What are the keys to successful management of this case?

Having L‐PRF supporting wound healing.What are the primary limitations to success in this case?

Having sufficient bone apical to the existing socket and at adjacent teeth.
Videoconferencing versus face‐to‐face psychotherapy: Insights from patients and psychotherapists about comparability of therapeutic alliance, empathy and treatment characteristics
Authors: Leuchtenberg Sophie , Gromer Daniel , Käthner Ivo 
Published: May 2022

AbstractObjectiveVideoconferencing psychotherapy (VCT) is an effective treatment option. Yet, it is unclear whether a similar therapeutic alliance as in conventional face‐to‐face psychotherapy (F2F) can be achieved, since previous studies yielded mixed results. Furthermore, surveys about the attitudes towards VCT amongst patients have been missing until now. The current study gathered opinions from patients and psychotherapists about the perceived comparability of VCT and F2F regarding contextual factors and treatment characteristics, focusing on therapeutic alliance and empathy.MethodAn online survey amongst patients (N = 189) and practitioners (N = 57) taking part in cognitive behavioural therapy was conducted after the first lockdown in Germany due to the COVID‐19 pandemic and a resulting transition from F2F to VCT for most participants.ResultsWhile patients experienced therapeutic alliance and empathy as comparable, psychotherapists indicated advantages of F2F. Both groups indicated advantages of F2F for the therapy contents and expressed advantages of VCT for flexibility regarding location and time. More than half of the participants expressed a preference for a combination of analogue and digital therapy.ConclusionThe perceived disadvantages of VCT can be addressed, for example, with training programmes for psychotherapists targeting communication in VCT and adapting established psychotherapy methods to a digital format to further improve VCT.
Beyond one size fits all? An experimental study of the effects of stage‐specific interventions to promote ecological online food shopping
Authors: Berger Verena , Burkhalter Linda 
Published: May 2022

AbstractEncouraging people to consume sustainably is more important than ever to tackle climate change. In the area of nutrition, we focused on understanding how social norm inspired treatments can effectively be applied to promote sustainable purchasing without restricting choice. Many intervention studies in this area have applied ‘one size fits all’‐measures, ignoring the target group's context and psycho‐social preconditions. To examine the effectiveness of tailored interventions, we tested the impact on purchasing decisions of four treatments. The treatments were developed based on the four stages of behavioural change that conceptualise behavioural change as a transition through a sequence of stages: predecision, preaction, action, and postaction. In an online experiment (N = 855), these treatments (shopping assistant, success story, commitment, and feedback) were integrated into a true‐to‐the‐original online food shop and socio‐psychological constructs were collected using a downstream questionnaire. The results of a regression analysis showed that there are differences in the effectiveness of the treatments on participants' purchasing decisions. The feedback treatment turned out to be a particularly effective measure for encouraging large numbers of people at different stages of behavioural change to select greener products. In line with theory, the impact of several socio‐psychological variables designed to encourage more eco‐friendly purchases increased from stage to stage. The results may motivate online food shop providers to create customer experiences that promote eco‐friendly consumption. At the same time, it should also encourage other researchers working in this field to develop effective measures that support the achievement of sustainability goals.
Immobilization Method to Separate Microalgae Biomass for Fatty Acid Methyl Ester Production
Authors: Sepian Noor Raihana Abu , Yasin Nur Hidayah Mat , Ramesh Nagaarasan 
Published: May 2022

AbstractAn immobilization method for simplified separation of cultured cells and their products from the growth media was developed. The growth rates of both immobilized and free cells of the microalga Chlorella vulgaris were compared. The free and immobilized cells reached nearly identical cell densities. The reported immobilization strategy uses a combination of matrices (sodium alginate (SA), calcium alginate (CA), and sodium carboxymethyl cellulose (CMC)) at different matrix/microalgae volumetric ratios of 0.3:1 and 1:1. The microalgae in the SACACMC/Mc (0.3:1) beads achieved the highest cell density. The cells immobilized in SACACMC/Mc (0.3:1) gave the highest lipid yield, as compared to the cells immobilized in SA. Pore size and membrane thickness analysis as well as surface images of SACACMC/Mc (0.3:1) showed that the mixed matrix had a unique structure favoring lipid production.
Influence of Flange Structure Parameters on Contact Pressure of the New‐Type Wedge Ring Gasket
Authors: Xu Wenbin 
Published: May 2022

AbstractAs a sealing element, the new‐type wedge ring gasket required the usage of flanges to achieve sealing. On the basis of previous research, the influence of flange structural parameters on the contact pressure of the sealing surface of the new wedge ring gasket was studied by finite element analysis. The effective thickness of the flange had the greatest influence on the contact pressure of the main and slave sealing surfaces, followed by the thickness of the big end of the cone neck, the outer diameter of the flange, and the height of the flange neck. The flange structure parameters were optimized by an orthogonal experiment, and the maximum contact pressure of the sealing surface of the gasket was obtained under optimized conditions. The results provided a basis for the selection of structural parameters of the flange used with the new‐type wedge ring gasket.
Deep Eutectic Solvents and Layered Double Hydroxides in Solid‐Phase Extraction of Antidepressant Drugs
Authors: Khezeli Tahere , Daneshfar Ali 
Published: May 2022

AbstractFor the first time, an acidic deep eutectic solvent (DES) comprised of malonic acid and ChCl was used to complete dissolution of Co‐Al, Ni‐Al, and Cu‐Al layered double hydroxides (LDHs) as sorbents in the vortex‐assisted in‐situ solid‐phase extraction of doxepin and amitriptyline followed by high‐performance liquid chromatography‐ultraviolet (HPLC‐UV). Moreover, the ability of urea:ChCl and ethylene glycol:ChCl was investigated to dissolve the LDH sorbents. The extraction conditions were optimized in terms of vortex time, molar ratio of Co2+:Al3+, NaOH concentration, and volume of DES based on the Box‐Behnen design. The linearity, limit of detection, and coefficient of determination R2 for both analytes were assessed as well. Recoveries of more than 93.1 % with relative standard deviations of less than 5.3 % were achieved for spiking urine and pharmaceutical formulation samples.
Total calcaneal allograft reconstruction of an Ewing's sarcoma in a child: Outcome and review of the literature
Authors: Torner Ferran , Nuñez Jorge H. , Inarejos Clemente Emilio José , Garraus Moira , Suñol Mariona , Martínez Aníbal D. , Moreno David 
Published: May 2022

AbstractBackgroundEwing's sarcoma rarely presents in bones of the feet. Surgical management usually includes amputation. Limb sparing surgery is anecdotal.CaseWe report the case of a 13‐year‐old boy with an Ewing sarcoma in his calcaneus who had a calcaneal reconstruction with total calcaneus allograft after induction chemotherapy.ConclusionsAt 42 months of follow‐up our patient remains disease free and functionally intact. A review of the exceptional limb salvage procedure options for malignant calcaneus tumor was performed.
Hybrid Whale Tabu algorithm optimized convolutional neural network architecture for intrusion detection in big data
Authors: Ponmalar A. , Dhanakoti V. 
Published: May 2022

AbstractThe rate of internet traffic in the digital world has expanded fast due to technological improvement. Because of the large number of internet users, there is a large volume of network traffic, and it is the most common and challenging task to evaluate, process, and store the data. The intrusion detection systems enhance the performance of early attacks prediction and malicious attack detection. The exiting techniques met a few challenges in terms of feature selection, computational cost, accuracy, higher dimensionality, computational time and so forth. To solve these issues, we proposed a novel deep learning model for intrusion detection in big data. The data was collected from NSL‐KDD, KDD‐Cup99, and UNSW‐NB15 datasets. Initially, the big data framework with a testbed is set up for big data analytics and processing in which the required information from a large volume is extracted. The datasets are stored in the Hadoop Distributed File System, which reduces the number of latencies in the distributed process. Finally, the intrusion detection from big data is performed using convolutional neural network‐based Hybrid Whale Tabu Optimization algorithm and it effectively classifies whether the data is intrusion is non‐intrusion. The proposed method provided appropriate and superior experimental results using the state‐of‐art method with various performance measures such as accuracy, Matthews correlation coefficient, balanced accuracy, specificity, sensitivity, precision, and F‐measures.
SPICE synthesis of a solar cell model with irradiance and temperature evaluation
Authors: Zenteno‐Franco Samuel , Gonzalez‐Diaz Victor R. , Ambrosio‐Lazaro Roberto C. , Guerrero‐Castellanos J. Fermi , Mino‐Aguilar Gerardo 
Published: May 2022

SummaryThis manuscript resumes the synthesis of a reliable electrical solar cell model in LTspice. The model improves correspondence with the physical I‐V and P‐V behavior, evaluating the temperature and irradiance dependence with simple approximations. A few electrical parameters into script lines synthesize the nonlinear branch relationship of a voltage‐controlled current source. The LTspice model includes new factors to improve the irradiance and temperature variation evaluation, which is uniform among solar cells.The results exhibit an average error of less than seven percent in an open‐source SPICE solution, similar to cumbersome computational efforts. The SPICE model enables the design of photovoltaic systems taking into account the temperature and irradiance factors with electrical components.
Heterogeneity and genomic loci of ubiquitous transgenic Cre reporter lines in zebrafish
Authors: Lalonde Robert L. , Kemmler Cassie L. , Riemslagh Fréderike W. , Aman Andrew J. , Kresoja‐Rakic Jelena , Moran Hannah R. , Nieuwenhuize Susan , Parichy David M. , Burger Alexa , Mosimann Christian 
Published: May 2022

AbstractBackgroundThe most‐common strategy for zebrafish Cre/lox‐mediated lineage labeling experiments combines ubiquitously expressed, lox‐based Switch reporter transgenes with tissue‐specific Cre or 4‐OH‐Tamoxifen‐inducible CreERT2 driver lines. Although numerous Cre driver lines have been produced, only a few broadly expressed Switch reporters exist in zebrafish and their generation by random transgene integration has been challenging due to position‐effect sensitivity of the lox‐flanked recombination cassettes. Here, we compare commonly used Switch reporter lines for their recombination efficiency and reporter expression pattern during zebrafish development.ResultsUsing different experimental setups, we show that ubi:Switch and hsp70l:Switch outperform current generations of the two additional Switch reporters actb2:BFP‐DsRed and actb2:Stop‐DsRed. Our comparisons also document preferential Cre‐dependent recombination of ubi:Switch and hsp70l:Switch in distinct zebrafish tissues at early developmental stages. To investigate what genomic features may influence Cre accessibility and lox recombination efficiency in highly functional Switch lines, we mapped these transgenes and charted chromatin dynamics at their integration sites.ConclusionsOur data documents the heterogeneity among lox‐based Switch transgenes toward informing suitable transgene selection for lineage labeling experiments. Our work further proposes that ubi:Switch and hsp70l:Switch define genomic integration sites suitable for universal transgene or switch reporter knock‐in in zebrafish.
Pelagic calcifiers face increased mortality and habitat loss with warming and ocean acidification
Authors: Bednaršek Nina , Carter Brendan R. , McCabe Ryan M. , Feely Richard A. , Howard Evan , Chavez Francisco P. , Elliott Meredith , Fisher Jennifer L. , Jahncke Jaime , Siegrist Zach 
Published: May 2022

AbstractGlobal change is impacting the oceans in an unprecedented way, and multiple lines of evidence suggest that species distributions are changing in space and time. There is increasing evidence that multiple environmental stressors act together to constrain species habitat more than expected from warming alone. Here, we conducted a comprehensive study of how temperature and aragonite saturation state act together to limit Limacina helicina, globally distributed pteropods that are ecologically important pelagic calcifiers and an indicator species for ocean change. We co‐validated three different approaches to evaluate the impact of ocean warming and acidification (OWA) on the survival and distribution of this species in the California Current Ecosystem. First, we used colocated physical, chemical, and biological data from three large‐scale west coast cruises and regional time series; second, we conducted multifactorial experimental incubations to evaluate how OWA impacts pteropod survival; and third, we validated the relationships we found against global distributions of pteropods and carbonate chemistry. OWA experimental work revealed mortality increases under OWA, while regional habitat suitability indices and global distributions of L. helicina suggest that a multi‐stressor framework is essential for understanding pteropod distributions. In California Current Ecosystem habitats, where pteropods are living close to their thermal maximum already, additional warming and acidification through unabated fossil fuel emissions (RCP 8.5) are expected to dramatically reduce habitat suitability.
Species traits explain public perceptions of human–bird interactions
Authors: Andrade Riley , Larson Kelli L. , Franklin Janet , Lerman Susannah B. , Bateman Heather L. , Warren Paige S. 
Published: May 2022

AbstractThe impacts of urbanization on bird biodiversity depend on human–environment interactions that drive land management. Although a commonly studied group, less attention has been given to public perceptions of birds close to home, which can capture people's direct, everyday experiences with urban biodiversity. Here, we used ecological and social survey data collected in the metropolitan region of Phoenix, Arizona, USA, to determine how species traits are related to people's perceptions of local bird communities. We used a trait‐based approach to classify birds by attributes that may influence human–bird interactions, including color, size, foraging strata, diet, song, and cultural niche space based on popularity and geographic specificity. Our classification scheme using hierarchical clustering identified four trait categories, labeled as Metropolitan (gray, loud, seedeaters foraging low to ground), Familiar (yellow/brown generalist species commonly present in suburban areas), Distinctive (species with distinguishing appearance and song), and Hummingbird (hummingbird species, small and colorful). Strongly held beliefs about positive or negative traits were also more consistent than ambivalent ones. The belief that birds were colorful and unique to the regional desert environment was particularly important in fortifying perceptions. People largely perceived hummingbird species and birds with distinctive traits positively. Similarly, urban‐dwelling birds from the metropolitan trait group were related to negative perceptions, probably due to human–wildlife conflict. Differences arose across sociodemographics (including income, age, education, and Hispanic/Latinx identity), but explained a relatively low amount of variation in perceptions compared with the bird traits present in the neighborhood. Our results highlight how distinctive aesthetics, especially color and song, as well as traits related to foraging and diet drive perceptions. Increasing people's direct experiences with iconic species tied to the region and species with distinguishing attributes has the potential to improve public perceptions and strengthen support for broader conservation initiatives in and beyond urban ecosystems.
Expanding the temporal and spatial scales of environmental DNA research with autonomous sampling
Authors: Truelove Nathan K. , Patin Nastassia V. , Min Markus , Pitz Kathleen J. , Preston Chris M. , Yamahara Kevan M. , Zhang Yanwu , Raanan Ben Y. , Kieft Brian , Hobson Brett , Thompson Luke R. , Goodwin Kelly D. , Chavez Francisco P. 
Published: May 2022

AbstractEnvironmental DNA (eDNA) is an emerging and powerful method for use in marine research, conservation, and management, yet time‐ and resource‐intensive protocols limit the scale of implementation. Long‐range autonomous underwater vehicles equipped with autonomous environmental sample processors (LRAUV‐ESPs) provide a new means for scaling up marine eDNA sample collection and processing. Here, we used eDNA metabarcoding of four marker genes (mitochondrial 12S rRNA, bacterial and archaeal 16S rRNA, nuclear 18S rRNA, and mitochondrial COI), which encompass the diversity of marine species from microbes to vertebrates, to demonstrate the efficacy of an LRAUV‐ESP in sampling eDNA and assessing community structure in the Monterey Bay National Marine Sanctuary. The sequencing results from samples that were autonomously collected were comparable with those collected from a ship at similar locations, times, and depths, supporting previous results that found no significant differences using targeted qPCR. This study demonstrates the potential of equipping autonomous underwater vehicles with ESPs to greatly expand the scale of eDNA sample collection and processing and provide much needed information regarding the changing spatial and temporal patterns of marine biodiversity, especially in many data‐poor regions of the world's oceans.