bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANALYSIS OF MARKETING MANAGEMENT IN INCREASING SAVING PRODUCTS IN KSPPS BMT NU, MLANDINGAN BRANCH, SITUBONDO
Authors: Rizal Sofian Syaiful , Dewi Mamik Sari 
Published: June 2022

Marketing management is the key to the continuity of an organization’s business so that it runs according to its function. The development of financial institutions, both banks, and non-banks, will encourage financial institutions always to optimize their marketing activities. Analyzing the market and its environment to find out how big the opportunity is to seize the market and how big the threat is that must be faced. This article aims to find out how marketing management is in improving products at the BMT NU Mlandingan branch. This study uses a descriptive qualitative method by conducting structured interviews in the form of questions related to obtaining relevant informants from customers and employees of the BMT NU Mlandingan Branch. Marketing management process by doing market segmentation, target market, determination, positioning, and differentiation. Savings and financing products are good and increasing from the intensity of increasing the number of customers with the reason that customers are easier and understand the advantages of savings and financing products at the malandingan branch of BMT NU. The findings in this study indicate that promotions can increase the number of customers in a faster time and reach a wider audience using existing media. Keywords: Marketing management, Products, BMT
In vivo Assessment of Localised Corneal Biomechanical Deterioration With Keratoconus Progression
Authors: Lopes Bernardo T. , Padmanabhan Prema , Eliasy Ashkan , Zhang Haixia , Abass Ahmed , Elsheikh Ahmed 
Published: June 2022

Purpose: To evaluate the regional corneal biomechanical deterioration with keratoconus (KC) progression as measured by the Stress-Strain Index (SSI) maps.Methods: The preoperative examinations of 29 progressive KC cases that were submitted to corneal cross-linking (CXL) were evaluated. The examinations included the tomography and the SSI measured by the Pentacam HR and the Corvis ST (Oculus, Wetzlar, Germany), respectively. The results were recorded twice, the latter of which was at the last visit before the CXL procedure. The patient-specific SSI maps were built, using data at each examination, based on finite element modelling and employing inverse analysis to represent the regional variation of biomechanical stiffness across the cornea.Results: All cases presented significant shape progression (above the 95% CI of repeatability) in anterior and posterior curvatures and minimum thickness. The overall corneal stiffness as measured by the SSI within the central 8 mm-diameter area underwent slight but significant reductions from the first to the last examination (−0.02 ± 0.02, range: −0.09 to 0, p &lt; 0.001). In all 29 cases, the reduction in stiffness was localised and concentred in the area inside the keratoconus cone. The SSI values inside the cone were significantly lower in the last examination (by 0.15 ± 0.09, range: −0.42 to −0.01, p &lt; 0.001), while the SSI outside the cone presented minimal, non-significant variations (0 ± 0.01, range: −0.04 to 0.01, p = 0.999).Conclusion: It has been observed through the SSI maps that the regional deterioration in stiffness was concerted inside the area of pathology, while only mild non-significant alterations were observed outside the area of pathology.
Metabolic Engineering of Bacillus amyloliquefaciens to Efficiently Synthesize L-Ornithine From Inulin
Authors: Zhu Yifan , Hu Yi , Yan Yifan , Du Shanshan , Pan Fei , Li Sha , Xu Hong , Luo Zhengshan 
Published: June 2022

Bacillus amyloliquefaciens is the dominant strain used to produce γ-polyglutamic acid from inulin, a non-grain raw material. B. amyloliquefaciens has a highly efficient tricarboxylic acid cycle metabolic flux and glutamate synthesis ability. These features confer great potential for the synthesis of glutamate derivatives. However, it is challenging to efficiently convert high levels of glutamate to a particular glutamate derivative. Here, we conducted a systematic study on the biosynthesis of L-ornithine by B. amyloliquefaciens using inulin. First, the polyglutamate synthase gene pgsBCA of B. amyloliquefaciens NB was knocked out to hinder polyglutamate synthesis, resulting in the accumulation of intracellular glutamate and ATP. Second, a modular engineering strategy was applied to coordinate the degradation pathway, precursor competition pathway, and L-ornithine synthesis pathway to prompt high levels of intracellular precursor glutamate for l-ornithine synthesis. In addition, the high-efficiency L-ornithine transporter was further screened and overexpressed to reduce the feedback inhibition of L-ornithine on the synthesis pathway. Combining these strategies with further fermentation optimizations, we achieved a final L-ornithine titer of 31.3 g/L from inulin. Overall, these strategies hold great potential for strengthening microbial synthesis of high value-added products derived from glutamate.
Synergistic Regulation of S-Vacancy of MoS2-Based Materials for Highly Efficient Electrocatalytic Hydrogen Evolution
Authors: Li Xiao-Yun , Zhu Shao-Ju , Wang Yi-Long , Lian Tian , Yang Xiao-yu , Ye Cui-Fang , Li Yu , Su Bao-Lian , Chen Li-Hua 
Published: June 2022

Low or excessively high concentration of S-vacancy (CS-vacancy) is disadvantageous for the hydrogen evolution reaction (HER) activity of MoS2-based materials. Additionally, alkaline water electrolysis is most likely to be utilized in the industry. Consequently, it is of great importance for fine-tuning CS-vacancy to significantly improve alkaline hydrogen evolution. Herein, we have developed a one-step Ru doping coupled to compositing with CoS2 strategy to precisely regulate CS-vacancy of MoS2-based materials for highly efficient HER. In our strategy, Ru doping favors the heterogeneous nucleation and growth of CoS2, which leads to a high crystallinity of Ru-doped CoS2 (Ru-CoS2) and rich heterogeneous interfaces between Ru-CoS2 and Ru-doped MoS2-x (Ru-MoS2-x). This facilitates the electron transfer from Ru-CoS2 to Ru-MoS2-x, thereby increasing CS-vacancy of MoS2-based materials. Additionally, the electron injection effect increases gradually with an increase in the mass of Co precursor (mCo), which implies more S2- leaching from MoS2 at higher mCo. Subsequently, CS-vacancy of the as-synthesized samples is precisely regulated by the synergistic engineering of Ru doping and compositing with CoS2. At CS-vacancy = 17.1%, a balance between the intrinsic activity and the number of exposed Mo atoms (EMAs) to boost highly active EMAs should be realized. Therefore, the typical samples demonstrate excellent alkaline HER activity, such as a low overpotential of 170 mV at 100 mA cm−2 and a TOF of 4.29 s−1 at -0.2 V. Our results show promise for important applications in the fields of electrocatalysis or energy conversion.
Study of the Effect of the Driving Force on the Kinetics of CO2 Hydrate Growth in Coal Particles
Authors: Chuanhai Liu , Ran Chen , Baoyong Zhang , Qiang Wu , Qiang Zhang , Qiong WU 
Published: June 2022

Coal and gas outbursts are geological disasters occurring in the process of coal mining that can cause serious casualties and economic losses, among which CH4 is the main component of coal mine gas. However, there are still many coal seams around the world that are dominated by CO2. Although the frequency of CO2 gas outburst accidents is relatively low, CO2 outbursts are very violent, notably difficult to control and highly dangerous. The application of hydration curing technology to reduce the pressure and gradient of CO2 gas in the coal can effectively reduce the occurrence of coal and CO2 outburst. Accordingly, in this paper, experimental studies on the growth kinetics of CO2 hydrate with three driving forces (2, 2.5, 3 MPa) were carried out under four different coal particle sizes (C1: 0.425–0.850 mm, C2: 0.250–0.425 mm, C3: 0.180–0.250 mm, C4: 0–0.180 mm) to obtain kinetic parameters such as gas consumption, growth rate, and heat of decomposition during the synthesis of CO2 hydrate. The results show that the hydrate nucleation time in the same particle size system does not follow the same decreasing trend with increasing driving force. Gas consumption of CO2 hydrates in the same particle size system increased with increasing driving force, and there exists a critical value regarding the effect of the driving force on CO2 hydrate generation in coal particles with the particle size. Under the same temperature conditions, increasing the driving force in the particle size system could increase the CO2 hydrate growth rate. With decreasing coal particle size and increasing driving force, the promoting effect gradually exceed the inhibiting effect, which promote CO2 hydrate formation. Through linear fitting, an equation of the average growth rate of CO2 hydrates versus the driving force for the C1-C4 systems is fitted to provide a reference to predict the average CO2 hydrate growth rate. In the same medium, with increasing driving force, more heat is required for complete decomposition, which remains relatively stable, and the heat of decomposition of CO2 hydrates is the highest in the C1 medium, indicating that the presence of CO2 hydrates in the C1 system represents the most stable state.
Unfolded Protein Response–Related Signature Associates With the Immune Microenvironment and Prognostic Prediction in Osteosarcoma
Authors: Zhang Zhao , Liu Xincheng , Cheng Debin , Dang Jingyi , Mi Zhenzhou , Shi Yubo , Wang Lei , Fan Hongbin 
Published: June 2022

Background: Osteosarcoma is a highly malignant bone tumor commonly occurring in adolescents with a poor 5-year survival rate. The unfolded protein response (UPR) can alleviate the accumulation of misfolded proteins to maintain homeostasis under endoplasmic reticulum stress. The UPR is linked to the occurrence, progression, and drug resistance of tumors. However, the function of UPR-related genes (UPRRGs) in disease progression and prognosis of osteosarcoma remains unclear.Methods: The mRNA expression profiling and corresponding clinical features of osteosarcoma were acquired from TARGET and GEO databases. Consensus clustering was conducted to confirm different UPRRG subtypes. Subsequently, we evaluated the prognosis and immune status of the different subtypes. Functional analysis of GO, GSEA, and GSVA was used to reveal the molecular mechanism between the subtypes. Finally, four genes (STC2, PREB, TSPYL2, and ATP6V0D1) were screened to construct and validate a risk signature to predict the prognosis of patients with osteosarcoma.Result: We identified two subtypes according to the UPRRG expression patterns. The subgroup with higher immune scores, lower tumor purity, and active immune status was linked to a better prognosis. Meanwhile, functional enrichment revealed that immune-related signaling pathways varied markedly in the two subtypes, suggesting that the UPR might influence the prognosis of osteosarcoma via influencing the immune microenvironment. Moreover, prognostic signature and nomogram models were developed based on UPRRGs, and the results showed that our model has an excellent performance in predicting the prognosis of osteosarcoma. qPCR analysis was also conducted to verify the expression levels of the four genes.Conclusion: We revealed the crucial contribution of UPRRGs in the immune microenvironment and prognostic prediction of osteosarcoma patients and provided new insights for targeted therapy and prognostic assessment of the disease.
Contribution of HLA and KIR Alleles to Systemic Sclerosis Susceptibility and Immunological and Clinical Disease Subtypes
Authors: Hanson Aimee L. , Sahhar Joanne , Ngian Gene-Siew , Roddy Janet , Walker Jennifer , Stevens Wendy , Nikpour Mandana , Assassi Shervin , Proudman Susanna , Mayes Maureen D. , Kenna Tony J. , Brown Matthew A. 
Published: June 2022

Systemic sclerosis (SSc) is an autoinflammatory, fibrotic condition of unknown aetiology. The presence of detectable autoantibodies against diverse nuclear antigens, as well as strong HLA associations with disease, suggest autoimmune involvement, however the links between endogenous and exogenous risk factors and SSc pathology remain undetermined. We have conducted a genetic analysis ofHLAinheritance in two independent and meta-analysed cohorts of 1,465 SSc cases and 13,273 controls, including stratified association analyses in clinical and autoantibody positive subgroups of disease. Additionally, we have used patient genotypes to impute gene dosages across theKIRlocus, encoding paired activating and inhibitory lymphocyte receptors for Class I HLA ligands, to conduct the largest analysis ofKIR-HLAepistatic interactions in SSc to date. We confirm previous Class II HLA associations with SSc risk and report a new Class I association with haplotypeHLA-B*44:03-HLA-C*16:01 at genome-wide significance (GWS). We further report statistically significantHLAassociations with clinical and serological subtypes of disease through direct case-case comparison, and report a new association ofHLA-DRB1*15:01, previously shown to bind topoisomerase-1 derived peptides, with anti-topoisomerase (ATA) positive disease. Finally, we identify genetic epistasis between KIRs and HLA class I ligands, suggesting genetic modulation of lymphocyte activation may further contribute to an individual’s underlying disease risk. Taken together, these findings support future functional investigation into endogenous immunological and environmental stimuli for disrupted immune tolerance in SSc.
Plasma CXCL14 as a Candidate Biomarker for the Diagnosis of Lung Cancer
Authors: Tian Peng-Fei , Ma Yu-Chen , Yue Dong-Sheng , Liang Fan , Li Chen-Guang , Chen Chen , Zhang Hua , Sun Xiao-Yan , Huang Wu-Hao , Zhang Zhen-Fa , Zhou Guang-Biao , Wang Gui-Zhen , Zhang Bin , Wang Chang-Li 
Published: June 2022

BackgroundEffective biomarkers for early diagnosis of lung cancer are needed. Previous studies have indicated positive associations between abnormal circulating cytokines and the etiology of lung cancer.MethodsBlood samples were obtained from 286 patients with pretreatment lung cancer and 80 healthy volunteers. Circulating cytokine levels were detected with a Luminex assay and enzyme-linked immunosorbent assay (ELISA). Urine samples were obtained from 284 patients and 122 healthy volunteers. CXC chemokine ligand 14 (CXCL14) expression in tumors and nontumor regions of lung tissues from 133 lung cancer cases was detected by immunohistochemical (IHC) staining and immunofluorescence (IF) staining of formalin fixed paraffin-embedded (FFPE) tissues.ResultsCompared with healthy volunteers, a 65.7-fold increase was observed in the level of CXCL14 in the plasma of lung cancer patients, and a 1.7-fold increase was observed in the level of CXCL14 in the urine of lung cancer patients, achieving a 0.9464 AUC (area under the curve) value and a 0.6476 AUC value for differentiating between lung cancer patients and healthy volunteers, respectively. Stromal CXCL14 expression was significantly associated with advanced pathologic stage (P&lt;0.001), pathologic N stage (P&lt;0.001), and recurrence and metastasis (P=0.014). Moreover, multivariate analysis suggested stromal CXCL14 expression as an independent predictor of DFS and OS.ConclusionsOur study demonstrates that CXCL14 might serve as a potential diagnostic and prognostic biomarker in patients with lung cancer.ImpactCXCL14 might serve as a potential diagnostic and prognostic biomarker in patients with lung cancer.
Extracellular Volume Fraction Derived From Dual-Layer Spectral Detector Computed Tomography for Diagnosing Cervical Lymph Nodes Metastasis in Patients With Papillary Thyroid Cancer: A Preliminary Study
Authors: Zhou Yan , Geng Di , Su Guo-Yi , Chen Xing-Biao , Si Yan , Shen Mei-Ping , Xu Xiao-Quan , Wu Fei-Yun 
Published: June 2022

ObjectivesThe current study evaluates the performance of dual-energy computed tomography (DECT) derived extracellular volume (ECV) fraction based on dual-layer spectral detector CT for diagnosing cervical lymph nodes (LNs) metastasis from papillary thyroid cancer (PTC) and compares it with the value of ECV derived from conventional single-energy CT (SECT).MethodsOne hundred and fifty-seven cervical LNs (81 non-metastatic and 76 metastatic) were recruited. Among them, 59 cervical LNs (27 non-metastatic and 32 metastatic) were affected by cervical root artifact on the contrast-enhanced CT images in the arterial phase. Both the SECT-derived ECV fraction (ECVS) and the DECT-derived ECV fraction (ECVD) were calculated. A Pearson correlation coefficient and a Bland–Altman analysis were performed to evaluate the correlations between ECVD and ECVS. Receiver operator characteristic curves analysis and the Delong method were performed to assess and compare the diagnostic performance.ResultsECVD correlated significantly with ECVS (r = 0.925; p &lt;0.001) with a small bias (−0.6). Metastatic LNs showed significantly higher ECVD (42.41% vs 22.53%, p &lt;0.001) and ECVS (39.18% vs 25.45%, p &lt;0.001) than non-metastatic LNs. By setting an ECVD of 36.45% as the cut-off value, optimal diagnostic performance could be achieved (AUC = 0.813), which was comparable with that of ECVS (cut-off value = 34.99%; AUC = 0.793) (p = 0.265). For LNs affected by cervical root artifact, ECVD also showed favorable efficiency (AUC = 0.756), which was also comparable with that of ECVS (AUC = 0.716) (p = 0.244).ConclusionsECVD showed a significant correlation with ECVS. Compared with ECVS, ECVD showed comparable performance in diagnosing metastatic cervical LNs in PTC patients, even though the LNs were affected by cervical root artifacts on arterial phase CT.
18F-Fluorodeoxyglucose Positron Emission Tomography of Head and Neck Cancer: Location and HPV Specific Parameters for Potential Treatment Individualization
Authors: Zschaeck Sebastian , Weingärtner Julian , Lombardo Elia , Marschner Sebastian , Hajiyianni Marina , Beck Marcus , Zips Daniel , Li Yimin , Lin Qin , Amthauer Holger , Troost Esther G. C. , van den Hoff Jörg , Budach Volker , Kotzerke Jörg , Ferentinos Konstantinos , Karagiannis Efstratios , Kaul David , Gregoire Vincent , Holzgreve Adrien , Albert Nathalie L. , Nikulin Pavel , Bachmann Michael , Kopka Klaus , Krause Mechthild , Baumann Michael , Kazmierska Joanna , Cegla Paulina , Cholewinski Witold , Strouthos Iosif , Zöphel Klaus , Majchrzak Ewa , Landry Guillaume , Belka Claus , Stromberger Carmen , Hofheinz Frank 
Published: June 2022

Purpose18F-fluorodeoxyglucose positron emission tomography (FDG-PET) is utilized for staging and treatment planning of head and neck squamous cell carcinomas (HNSCC). Some older publications on the prognostic relevance showed inconclusive results, most probably due to small study sizes. This study evaluates the prognostic and potentially predictive value of FDG-PET in a large multi-center analysis.MethodsOriginal analysis of individual FDG-PET and patient data from 16 international centers (8 institutional datasets, 8 public repositories) with 1104 patients. All patients received curative intent radiotherapy/chemoradiation (CRT) and pre-treatment FDG-PET imaging. Primary tumors were semi-automatically delineated for calculation of SUVmax, SUVmean, metabolic tumor volume (MTV) and total lesion glycolysis (TLG). Cox regression analyses were performed for event-free survival (EFS), overall survival (OS), loco-regional control (LRC) and freedom from distant metastases (FFDM).ResultsFDG-PET parameters were associated with patient outcome in the whole cohort regarding clinical endpoints (EFS, OS, LRC, FFDM), in uni- and multivariate Cox regression analyses. Several previously published cut-off values were successfully validated. Subgroup analyses identified tumor- and human papillomavirus (HPV) specific parameters. In HPV positive oropharynx cancer (OPC) SUVmax was well suited to identify patients with excellent LRC for organ preservation. Patients with SUVmax of 14 or less were unlikely to develop loco-regional recurrence after definitive CRT. In contrast FDG PET parameters deliver only limited prognostic information in laryngeal cancer.ConclusionFDG-PET parameters bear considerable prognostic value in HNSCC and potential predictive value in subgroups of patients, especially regarding treatment de-intensification and organ-preservation. The potential predictive value needs further validation in appropriate control groups. Further research on advanced imaging approaches including radiomics or artificial intelligence methods should implement the identified cut-off values as benchmark routine imaging parameters.
Delayed Diagnosis and Multi-TKI Intolerance: A Case Report of CML Concurrent With COVID-19
Authors: Luan Chengxin , Wang Haixia , Zhou Junjie , Ma Xiaoyu , Long Zhangbiao , Cheng Xin , Chen Xiaowen , Xia Ruixiang , Ge Jian 
Published: June 2022

IntroductionThe hematological manifestations of corona virus disease 2019 (COVID-19) can confound the diagnosis and therapy of other diseases. In this paper, we firstly reported a case of chronic myeloid leukemia (CML) of delayed diagnosis and intolerance to tyrosine kinase inhibitors (TKIs) concurrent with COVID-19.Case PresentationA 56-year-old female was diagnosed as COVID-19 with no obvious leukocytosis [white blood cell (WBC), ≤17 × 109/L] or splenomegaly until ablation of the virus. Bone marrow aspiration was conducted to establish the diagnosis of CML. She accepted an adjusted dosage of imatinib initially and had to suspend it after myelosuppression (day 41). After hematopoietic therapy, imatinib was given again (day 62), but she was still non-tolerant, and nilotinib at 150 mg twice a day was prescribed from day 214. At just about 4 weeks later, nilotinib was discontinued due to myelosuppression. Then, it was reduced to 150 mg per day and was re-initiated (day 349), but she was still non-tolerant to it. Similarly, from day 398, flumatinib at 200 mg per day was tried, but she was non-tolerant. Her white blood cell or platelet count fluctuated markedly with poor therapeutic response. Considering that she was relatively tolerant and responsive to imatinib, the medication was re-initiated at 200 mg and reduced to 100 mg per day. Her follow-up revealed stable WBC and PLT counts. The latest BCR-ABL-210/ABL was decreased to 0.68% at about 6 months after imatinib was re-initiated, which means an improved response.ConclusionThe offset effect between CML and SARS-CoV-2 infection was supposed to be the underlying mechanism for the absence of leukocytosis or splenomegaly. The impact of immune network by SARS-CoV-2 preserved and disrupted the patient’s response to TKIs despite the virus’ ablation. We suggest that a continued elevation of basophils may be a useful indicator for CML concurrent with COVID-19, and individualized treatment with adjusted dosage and suitable type of TKIs should be considered to improve the patient’s health outcome.