bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microtubule Associated Protein 4 Phosphorylation-Induced Epithelial-to-Mesenchymal Transition of Podocyte Leads to Proteinuria in Diabetic Nephropathy
Authors: Li Lingfei , Feng Yanhai , Zhang Junhui , Zhang Qiong , Ren Jun , Sun Cheng , Li Shujing , Lei Xia , Luo Gaoxing , Hu Jiongyu , Huang Yuesheng 
Published: January 2022

Abstract
Background

Diabetic nephropathy (DN) involves various structural and functional changes because of chronic glycemic assault and kidney failure. Proteinuria is an early clinical manifestation of DN, but the associated pathogenesis remains elusive. This study aimed to investigate the role of microtubule associated protein 4 (MAP4) phosphorylation (p-MAP4) in proteinuria in DN and its possible mechanisms.
Methods

In this study, the urine samples of diabetic patients and kidney tissues of streptozotocin (STZ)-induced diabetic mice were obtained to detect changes of microtubule associated protein 4 (MAP4) phosphorylation. A murine model of hyperphosphorylated MAP4 was established to examine the effect of MAP4 phosphorylation in DN. Podocyte was applied to explore changes of kidney phenotypes and potential mechanisms with multiple methods.
Results

Our results demonstrated elevated content of p-MAP4 in diabetic patients’ urine samples, and increased kidney p-MAP4 in streptozocin (STZ)-induced diabetic mice. Moreover, p-MAP4 triggered proteinuria with aging in mice, and induced epithelial-to-mesenchymal transition (EMT) and apoptosis in podocytes. Additionally, p-MAP4 mice were much more susceptible to STZ treatment and showed robust DN pathology as compared to wild-type mice. In vitro study revealed high glucose (HG) triggered elevation of p-MAP4, rearrangement of microtubules and F-actin filaments with enhanced cell permeability, accompanied with dedifferentiation and apoptosis of podocytes. These effects were significantly reinforced by MAP4 hyperphosphorylation, and were rectified by MAP4 dephosphorylation. Notably, pretreatment of p38/MAPK inhibitor SB203580 reinstated all HG-induced pathological alterations.
Conclusions

The findings indicated a novel role for p-MAP4 in causing proteinuria in DN. Our results indicated the therapeutic potential of MAP4 in protecting against proteinuria and related diseases.
Emerging And Legacy PFAS And Cytokine Homeostasis In Women of Childbearing Age
Authors: Nian Min , Zhou Wei , Feng Yan , Wang Yan , Chen Qian , Zhang Jun 
Published: January 2022

Abstract
The effect of legacy and emerging per- and polyfluoroalkyl substances (PFAS) on cytokine homeostasis in human remains poorly understood. This study aimed to evaluate the associations between legacy and emerging PFAS and cytokine profiles, and identify the main contributors to the disturbance of cytokine homeostasis. We quantified 21 PFAS in 198 Chinese women of childbearing age from 2015 to 2016. 13 cytokines were measured using the Meso Scale Discovery U-PLEX and V-PLEX platforms. The associations between PFAS exposure and cytokine levels were assessed using multiple linear regression (single-exposure), and Bayesian kernel machine regression (BKMR) models (PFAS mixture exposure). In single PFAS models, legacy and alternative PFAS were positively associated with Th1 and Treg cytokines, and negatively associated with Th2 and Th17 cytokines. For instance, each ln-unit increase in 6:2 chlorinated perfluoroalkyl ether sulfonic acid (6:2 Cl-PFESA), perfluorooctanoic acid (PFOA), and perfluorooctane sulfonate (PFOS) was associated with a decrease in IL-10 by -0.228 (95% CI: -0.336, -0.120), -0.153 (95% CI: -0.277, -0.030), and -0.174 (95% CI: -0.339, -0.010), respectively. The BKMR model showed a significantly positive association of PFAS mixture with TGF-β and a negative association with IL-10. Overall, these results indicate that both legacy and emerging PFAS may affect the homeostasis of cytokines.
The Relationship Between the Meaning of Life, Psychological Well-Being, Self-Care, and Social Capital, with Depression and Death Anxiety in the Elderly Living in Nursing Homes: The Mediating Role of Loneliness
Authors: Afrashteh Majid Yousefi , Majzoobi Mohammad Reza , Janjani Parisa , Forstmeier Simon 
Published: January 2022

Abstract

The current study aims to investigate the meaning of life, psychological well-being, self-care, and social capital, with depression and death anxiety in the elderly living in nursing homes through the mediating role of loneliness. The statistical population included all the elderly aged at least 60 years living in Tehran, Qazvin and Zanjan, Iran in 2020, among whom 489 (273 men and 216 women) were selected using convenience sampling method. Participants filled out Steger’s Meaning of Life, Ryff and Singer’s Psychological Well-Being Scale, Söderhamn et al.’s Self-Care Ability, Nahapiet and Ghoshal’s Social capital, Beck’s depression, Templer’s Death Anxiety, Russell et al.’s Loneliness questionnaires. The results indicated that there is a significant relationship between the meaning of life, psychological well-being, self-care, and social capital, with depression through the mediating role of loneliness. Besides, there was a significant relationship between the meaning of life, psychological well-being, self-care, and social capital, with death anxiety through the mediating role of loneliness. Moreover, the results of path analysis showed that the hypothesized model of the current study has an excellent fit in the study sample.
Land-sea Thermal Contrast in Relation With Summer Monsoon Onset Over the Chao Phraya River Basin
Authors: Yamada Tomohito J. , Shrivastava Sourabh , Kato Ryosuke 
Published: January 2022

Abstract
An earlier onset of the Southeast Asian summer monsoon (SAM) was observed over the Chao Phraya River basin in Thailand using Thai Meteorological Department (TMD)-derived high-resolution merged rainfall from 1981 to 2016. As the SAM is precipitous, its variability depends on many local and global factors, such as thermal conditions over the Bay of Bengal (BoB) and Tibetan Plateau (TbT). Despite tremendous studies in the past, the role of thermal heat contrast over SAM is still not fully understood. Using the observation and reanalysis datasets, it was found that the absolute value of total heat over the BoB was higher. However, the interannual variability in total heat is higher over the TbT. Significant changes in surface temperature (±1.5°C), air thickness (±20 meters) and geopotential height found over the TbT were associated with early (late) SAM onset. The results also suggested that the significant changes in air thickness were influenced by the surface temperature difference over the TbT, and the changes in the integrated apparent heat source and integrated apparent moisture sink were up to ± 100 Wm−2, which resulted in stronger (weaker) convective activities over the BoB and mainland of the Indochina Peninsula during early (late) SAM onset. At the intraseasonal timescale, the instance MJO found over the Indian Ocean and Western Hemisphere at 4 to 10 days span during early SAM onset. An opposite scenario is found for a late SAM onset years with MJO location over Western Pacific and Maritime continent.
Analysis On Ecological Characteristics of Mississippian Coral Reefs in Langping, Guangxi
Authors: Yang Dayong , Chang Honglun , Liu Xiao , Wan Peng , Shen Liming 
Published: January 2022

Abstract
Several Late Viséan-Serpukhovian coral reefs were identified in Langping, Tianlin. To further understand of environment that was suitable for the development of reef-building communities and the construction of coral reefs in Langping, in this paper, part of the reef-building environmental and the ecological characteristics of coral reefs then were recovered by analyzing the development settings, palaeogeography, sedimentation of reefs, the response to hydrodynamic conditions of reef-building corals, effects of disturbance and non-reef-building organism on reef communities, and the influence of coral morphology on reef development. The sedimentary environment of Langping in Late Viséan-Serpukhovian is considered to be suitable for the development of benthic communities. The current appearance of reefs is determined by both coral populations ecological characteristics and reef-building environment.
Striatal Syntaixin 1A Plays a Protective Role Against Iminodipropionitrile Induced Tic Disorder Through Interaction with Dopamine Transporter
Authors: Liu Xiumei , Wang Xueming , Zhang Xiaoling , Cao Aihua 
Published: January 2022

Abstract
An important mechanism of Tic disorder (TD) is dysfunction in the dopamine (DA) system. Our pilot observation found the expression of Syntaxin 1A (STX1A), a presynaptic SNARE complex, changed in the striatum of TD animals. The present study aimed to clarify the biological role of striatal STX1A in the pathological state of TD and the specific mechanism of its regulation of the dopaminergic system. The TD rat model was established using iminodipropionitrile (IDPN). Adenovirus was used to modulate the expression of STX1A and dopamine transporter (DAT) in vivo and vitro. Primary culture of striatal dopaminergic neurons was performed for in-vitro observation of the DA reuptake, CO-IP analysis of the interaction between STX1A and DAT. First, using immunofluorescence staining, Western blotting, and qPCR, we found that the IDPN induced TD model had reduced striatal STX1A expression. In vitro, the DA content in the supernatant was significantly lower in the STX1A overexpressed group, and the intracellular DA content was significantly higher. Overexpression of STX1A in vivo partially counteracts the IDPN-induced TD-like behaviors, including bite time and head shaking time. Meanwhile, in-vivo knockdown of STX1A can aggravates TD-like behaviors. Further, DAT was overexpressed in vivo, and the TD-like behavior was alleviated. Interestingly, overexpression of DAT in the striatum resulted in increased levels of STX1A. In order to clarify the interaction between DAT and STX1A, the CO-IP analysis was conducted based on the protein of purified striatal dopaminergic neurons. Compared to the IgG control, the blots of DAT and STX1A showed significant binding of each other. Striatal STX1A expression is decreased in TD development, and STX1A plays an anti-TD role possibly through interaction with DAT, which maintains the DA reuptake. The exorbitant DA signal caused by STX1A inhibition drives the pathological stereotyped behavior.
Flexible Minimally Invasive CARS Measurement Method with Tapered Optical Fiber Probe for Single-Cell Application
Authors: Wang Tong , Jiang Junfeng , Liu Kun , Wang Shuang , Niu Panpan , Liu Yize , Liu Tiegen 
Published: January 2022

Abstract
We proposed and demonstrated a flexible, endoscopic, and minimally invasive coherent anti-Raman Stokes scattering (CARS) measurement method for single-cell application, employing a tapered optical fiber probe. A few-mode fiber (FMF), whose generated four-wave mixing band is out of CARS signals, was selected to fabricate tapered optical fiber probes, deliver CARS excitation pulses, and collect CARS signals. The adiabatic tapered fiber probe with a diameter of 11.61 µm can focus CARS excitation lights without mismatch at the focal point. The measurements for proof-of-concept were made with methanol, ethanol, cyclohexane, and acetone injected into simulated cells. The experimental results show that the tapered optical fiber probe can detect carbon-hydrogen (C–H) bond-rich substances and their concentration. To our best knowledge, this optical fiber probe provides the minimum size among probes for detecting CARS signals. These results pave the way for minimally invasive live-cell detection in the future.
Construction of Nominal Ionospheric Gradient Using Satellite Pair Method Based on GNSS CORS Observation in Indonesia
Authors: supriadi slamet , Abidin Hasanuddin Zainal , Wijaya Dudy Darmawan , Abadi Prayitno , Saito Susumu , Prabowo Dwiko Unggul 
Published: January 2022

Abstract
Ground-Based Augmentation System (GBAS) is a GNSS augmentation system that meets International Civil Aviation Organization (ICAO) requirements to support precision approach and landing. GBAS is based on the local differential GNSS technique with reference stations located around the airport to provide necessary integrity and accuracy. The performance of the GBAS system can be affected by the gradient in the ionospheric delay between the aircraft and the reference stations. A nominal ionospheric gradient, which is bounded by a conservative error bound, is represented by a parameter σvig. σvig was commonly determined using station pair to GNSS Continuous Operating Reference Station (CORS) data. The station pair method is susceptible to doubling of receiver bias error and is not suitable with the CORS conditions in Indonesia. We propose a satellite pair method that is found to be more suitable for the CORS network over Indonesia which is centered in Java and Sumatra islands. The value of σvig (4.48 mm/km) is obtained using this method along with the preliminary results of a comparison of σvig from Java and Sumatra islands.
Demonstration of Dipole-Induced Transparency Using Mirrored Split-Ring Resonator Metasurface For Microwave Applications
Authors: VP Sarin , Raj Rohith K. , K Vasudevan 
Published: January 2022

Abstract
In this paper, dipole-induced transparency in the microwave regime is proposed and verified using experimental and simulation studies. A single layer mirrored Split-Ring Resonator (SRR) metasurface array working under the H⊥excitation scenario is used to achieveout-of-phase electric dipole moments on the metasurface for a normal incident plane wave. The emergence of the transparency window is accompanied by the destructive interference between out-of-phase oscillating electric dipole moments on the metasurfaceand is verified in computations by studying the radar Cross Section in full-wave electromagnetic simulations.We used the multipole scattering theory to validate the results computationally. The coupling effects are studied numerically, and the emergence of the transparency window is studied experimentally using transmission measurements inside an anechoic chamber using a vector network analyzer.
Probability of Reaction Pathways of Amine With Epoxides in the Reagent Ratio of 1:1 and 1:2
Authors: Borysenko Iryna O. , Okovytyy Sergiy I. , Leszczynski Jerzy 
Published: January 2022

Abstract
The algorithm for generating and estimating the probability of possible reaction pathways for multichannel bimolecular interactions was used to predict the reaction products in the reagent ratio of 1:1 and 1:2. Here we have considered the possible reaction pathways of the reaction of amine ((1S,2S,4S)-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (1) with epoxides (2-((cyclohexyloxy)methyl)oxirane (2), 2-(phenoxymethyl)oxirane (3), (N-(oxiran-2-ylmethyl)-N-phenylbenzenesulfonamide 8) in order to explain experimental observed data, which indicate differences in the reactivity of glycidyl ethers and glycidylsulfonamide with framework amines. Based on the proposed algorithm [39], we have investigated the reaction in the reagent ratio of 1:1 and 1: 2. Calculated values of activation barriers indicate a low probability of formation of interaction products of amine (1) with epoxide (8) with a (1:2) reagent ratio due to steric hindrances in the reaction center.
Gastrointestinal Bleeding During Direct Oral Anticoagulants Therapy in Patients With Nonvalvular Atrial Fibrillation and Risk of Polypharmacy
Authors: Honda Taku , Abe Koichiro , Oda Minoru , Harada Fumito , Maruyama Kyohei , Aoyagi Hitoshi , Miura Ryo , Konno Kumiko , Arizumi Toshihiko , Asaoka Yoshinari , Kodashima Shinya , Kozuma Ken , Yamamoto Takatsugu , Tanaka Atsushi 
Published: January 2022

Abstract
Although concomitant medications have been raised as a factor affecting hemorrhage during direct oral anticoagulants (DOACs) therapy, details remain unelucidated. This study was conducted to clarify the relationship between concomitant medications with possible pharmacokinetic interactions and number of concomitant medications, and bleeding and embolism in patients with nonvalvular atrial fibrillation on DOACs. The subjects were 1,010 patients prescribed DOACs between April 2011 and June 2018. The study investigated their course between the first prescription and December 2018, including the presence or absence of clinically relevant bleeding, gastrointestinal bleeding (GIB), and major cardiovascular and cerebrovascular events (MACCE). Impacts of medications were evaluated by the general linear model with inverse probability-weighted propensity score. The observation period was 2,272 patient-years. The rate of bleeding was 4.7%/year, GIB was 2.8%/year, and MACCE was 2.0%/year. Taking 10 or more oral medications concurrently was a significant risk for GIB (hazard ratio, 2.046 [1.188–3.526]; p = 0.010). Nonsteroidal anti-inflammatory drugs (NSAIDs) was the only significant risk for GIB. Clinicians should be aware of gastrointestinal bleeding when using DOACs with patients taking more than 10 medications and/or NSAIDs.
In-depth cardiovascular and pulmonary assessments in children with multisystem inflammatory syndrome after SARS-CoV-2 infection: a case series study
Authors: Astley Camilla , Badue Maria Fernanda , Lima Marcos Santos , Buchpiguel Carlos Alberto , Carneiro Camila G. , Sapienza Marcelo Tatit , Leal Gabriela Nunes , Prado Danilo Marcelo Leite do , Peçanha Tiago , Sieczkowska Sofia Mendes , Matsuo Olivia Mari , Lindoso Livia , Marques Heloisa Helena , Silva Clovis Artur , Gualano Bruno 
Published: January 2022

Abstract
Objective
To perform an in-depth assessment of cardiovascular and pulmonary outcomes in a series of 5 post discharged multisystem inflammatory syndrome (MIS-C) survivors.
Methods
Data were collected ≅1.9 month after hospital discharge at a tertiary hospital in São Paulo, Brazil. All patients (7-18 years; 3 females) fulfilled the MIS-C diagnosis according to CDC. The battery of tests included: 13 N-ammonia PET-CT imaging, standard echocardiography, brachial flow-mediated dilation using a Doppler ultrasound, cardiopulmonary exercise test, and blood markers.
Results
Upon PET-CT scans, two patients exhibited severe perfusion defect developed in the left ventricular cavity suggesting extensive myocardial ischemia, and one patient showed persistent mild pericardial effusion. Other two patients had endothelial dysfunction. All patients exhibited abnormal cardiopulmonary reserve during exercise (e.g., low VO2peak). Three patients had abnormal values for D-dimer and fibrinogen.
Conclusion
This study reveals novel pathological findings in MIS-C patients, which may help optimize treatment protocols in this condition.
Identification of a Signature Predicting Prognosis of Breast Cancer Patients Through Integrative Analysis of Single-cell and Bulk RNA Sequencing Data
Authors: Chen Hanghang , Tian Tian , Luo Haihua , Li Jinming , Jiang Yong 
Published: January 2022

Abstract
Background: Due to the high heterogeneity, it is still a challenge to precisely predict the prognosis of breast cancer (BRCA) patients. This study aimed to explore the crucial genes in tumourigenesis and prognosis-related molecular characteristics to predict the prognosis of breast cancer through a comprehensive analysis of single cell and bulk RNA-sequencing data.Methods: Gene expression data and corresponding clinical data of breast cancer from The Cancer Genome Atlas (TCGA) and Gene Expression Ominibus (GEO) datasets were collected. First, differentially expressed genes (DEGs) among trajectories from 412 cells of 11 BRCA patients were identified though PCA, t-SNE and single cell trajectory analyses. Second, we further explore the DEGs between normal and tumour tissues from the TCGA database. Then the intersection DEGs were used to construct a signature and the 4 independent GEO datasets were merged to validate the signature. Finally, gene set enrichment analysis (GSEA) and a website TIDE was employed to compare the immune-related cells, pathways, scores of immune escape with riskscores of our signature.Results: A prognostic signature comprising 20 genes was developed to divide patients into high-risk and low-risk groups, and its prognostic performance was excellent in two independent patient cohorts (n=1053, 620). The high-risk group generally had lower levels of immune cell infiltration, lower activity of immune pathway activity and higher scores of immune escape than did the low-risk group. Conclusion: A 20-gene signature was successfully constructed to assess the prognosis of breast cancer patients. The signature was also found closely associated with the tumour immune microenvironment and immune escape.
A Feasibility Study for Usefulness of Preoperative Simulation of Medial Open-wedge High Tibial Osteotomy for Predicting Postoperative Realignment
Authors: Konda Shoji , Ishibashi Teruya , Tamaki Masashi , Sugamoto Kazuomi , Tomita Tetsuya 
Published: January 2022

Abstract
Three-dimensional preoperative surgical realignment simulation of medial open-wedge high tibial osteotomy (OWHTO), in which simplified as the rigid rotation around the hinge axis, has been performed to predict the postoperative change and to develop a patient specific instrument for accurate osteotomy. However, the realistic practicality of this extremely simplified simulation method has not been verified. The purpose of this study was to investigate the usefulness of realignment simulation, in which medial OWHTO is simplified as a rotation around a hinge axis, in comparison with a postoperative CT model. Three-dimensional surface model of the tibia and femur was created from preoperative computed-tomography (CT) images (preoperative model) of three patients. Sixty computer simulation models of the medial OWHTO in each patient were created by realignment simulation, in which medial OWHTO is simplified as the rigid rotation of proximal part of tibia relative to the distal part from 1 degree to 20 degrees around three type of hinge axes. The simulation models were compared with the actual postoperative model created from postoperative CT images to assess the reality of the simulation model. After the distal parts of the tibia between each simulation model and postoperative CT model were aligned by a surface registration, average surface distance between two models was calculated as an index representing the similarity of the simulation model to the postoperative model. The minimum average surface distance between the simulation and postoperative CT models were almost 1mm in each patient. The rotation angles at which the minimum average surface distance was represented were almost identical to the actual correction angles. Overlaying the simulation and the postoperative CT models, we found that the posterior tibial tilt and the axial rotation of the proximal tibia of the simulation model well represented that of the postoperative CT model as well as the valgus correction. Therefore, the realignment simulation of medial OWHTO simplified as the rigid rotation around the hinge axis can generate the realistic candidates of postoperative realignment that includes the actual postoperative realignment, suggesting the usefulness for the preoperative simulation method.