bluuebunny/crossref_metadata_2025_split huggingface dataset:
A non-invasive multipoint product temperature measurement for pharmaceutical lyophilization
Authors: Jiang Xiaofan , Kazarin Petr , Sinanis Michael D. , Darwish Ahmad , Raghunathan Nithin , Alexeenko Alina , Peroulis Dimitrios 
Published: July 2022

AbstractMonitoring product temperature during lyophilization is critical, especially during the process development stage, as the final product may be jeopardized if its process temperature exceeds a threshold value. Also, in-situ temperature monitoring of the product gives the capability of creating an optimized closed-loop lyophilization process. While conventional thermocouples can track product temperature, they are invasive, limited to a single-point measurement, and can significantly alter the freezing and drying behavior of the product in the monitored vial. This work has developed a new methodology that combines non-invasive temperature monitoring and comprehensive modeling. It allows the accurate reconstruction of the complete temperature profile of the product inside the vial during the lyophilization process. The proposed methodology is experimentally validated by combining the sensors’ wirelessly collected data with the advanced multiphysics simulations. The flexible wireless multi-point temperature sensing probe is produced using micro-manufacturing techniques and attached outside the vial, allowing for accurate extraction of the product temperature.
Reprocessed magnetorheological elastomers with reduced carbon footprint and their piezoresistive properties
Authors: Munteanu A. , Ronzova A. , Kutalkova E. , Drohsler P. , Moucka R. , Kracalik M. , Bilek O. , Mazlan S. A. , Sedlacik M. 
Published: July 2022

AbstractDespite the vast amount of studies based on magnetorheological elastomers (MREs), a very limited number of investigations have been initiated on their reprocessing. This paper presents a new type of recyclable MRE which is composed of thermoplastic polyurethane (TPU) and carbonyl iron particles (CI). The chosen TPU can be processed using injection moulding (IM), followed by several reprocessing cycles while preserving its properties. Numerous types of injection moulded and reprocessed MREs have been prepared for various particle concentrations. The effect of thermo-mechanical degradation on the recycled MREs has been investigated while simulating the reprocessing procedure. An apparent decrease in molecular weight was observed for all the examined matrices during the reprocessing cycles. These changes are attributed to the intermolecular bonding between the hydroxyl groups on the surface of the CI particles and the matrix which is studied in depth. The effect of reprocessing and the presence of magnetic particles is evaluated via tensile test, magnetorheology and piezoresistivity. These characterization techniques prove that the properties of our MREs are preserved at an acceptable level despite using 100% of recyclates while in real applications only up to 30% of recycled material is generally used.
Key regulatory pathways, microRNAs, and target genes participate in adventitious root formation of Acer rubrum L
Authors: Zhu Wenpeng , Zhang Manyu , Li Jianyi , Zhao Hewen , Zhang Kezhong , Ge Wei 
Published: July 2022

AbstractRed maple (Acer rubrumL.) is a type of colorful ornamental tree with great economic value. Because this tree is difficult to root under natural conditions and the seedling survival rate is low, vegetative propagation methods are often used. Because the formation of adventitious roots (ARs) is essential for the asexual propagation ofA. rubrum, it is necessary to investigate the molecular regulatory mechanisms of AR formation inA. rubrum. To address this knowledge gap, we sequenced the transcriptome and small RNAs (sRNAs) of theA. rubrumvariety ‘Autumn Fantasy’ using high-throughput sequencing and explored changes in gene and microRNA (miRNA) expression in response to exogenous auxin treatment. We identified 82,468 differentially expressed genes (DEGs) between the treated and untreated ARs, as well as 48 known and 95 novel miRNAs. We also identified 172 target genes of the known miRNAs using degradome sequencing. Two key regulatory pathways (ubiquitin mediated proteolysis and plant hormone signal transduction),Ar-miR160aand the target geneauxin response factor 10(ArARF10) were selected based on KEGG pathway and cluster analyses. We further investigated the expression patterns and regulatory roles ofArARF10through subcellular localization, transcriptional activation, plant transformation, qRT-PCR analysis, and GUS staining. Experiments overexpressingArARF10andAr-miR160a, indicated thatArARF10promoted AR formation, whileAr-miR160ainhibited AR formation. Transcription factors (TFs) and miRNAs related to auxin regulation that promote AR formation inA. rubrumwere identified. Differential expression patterns indicated theAr-miR160a-ArARF10interaction might play a significant role in the regulation of AR formation inA. rubrum. Our study provided new insights into mechanisms underlying the regulation of AR formation inA. rubrum.
Neonatal outcomes in women with preterm premature rupture of membranes at periviable gestational age
Authors: Herzlich Jacky , Mangel Laurence , Halperin Ariel , Lubin Daniel , Marom Ronella 
Published: July 2022

AbstractTo examine the outcomes of preterm infants born to women with preterm premature rupture of membranes (PPROM) at periviable gestational age. This is an observational retrospective cohort study analyzing data collected on singleton deliveries complicated by prolonged premature rupture of membranes occurring between 17 and 33 weeks of gestation. Neonatal outcomes including birth weight, Apgar score, retinopathy of prematurity, intraventricular hemorrhage, bronchopulmonary dysplasia, necrotizing enterocolitis, hearing impairment and mortality were evaluated. Ninety-four preterm infants who were born after a prolonged premature rupture of membranes of at least 7 days were included in the study. Median gestational week at onset of membrane rupture was 27.1 ± 4.2 weeks (range 17–33) and median latency period in days was 16 ± 21.8 (range 7–105). The cohort was stratified by gestational week (GW) at onset of PPROM (group 1: 17–23, group 2: 24–27, and group 3: 28–33). We found that the survival rate to discharge within neonates born after prolonged rupture of membrane at gestational week less than 24 weeks is 79.2% and 88.9% in group 2. These neonates did not show an increased rate of major morbidities compared to neonates born following membrane rupture at gestational week 24 to 27. We described a high survival rate to discharge without major morbidities following prolonged preterm membrane rupture of at least 7 days of latency before viability.
Multi-omics study identifies novel signatures of DNA/RNA, amino acid, peptide, and lipid metabolism by simulated diabetes on coronary endothelial cells
Authors: Moreno-Ulloa Aldo , Delgado-De la Herrán Hilda Carolina , Álvarez-Delgado Carolina , Mendoza-Porras Omar , Carballo-Castañeda Rommel A. , Donis-Maturano Luis , Villarreal Francisco 
Published: July 2022

AbstractCoronary artery endothelial cells (CAEC) exert an important role in the development of cardiovascular disease. Dysfunction of CAEC is associated with cardiovascular disease in subjects with type 2 diabetes mellitus (T2DM). However, comprehensive studies of the effects that a diabetic environment exerts on this cellular type are scarce. The present study characterized the molecular perturbations occurring on cultured bovine CAEC subjected to a prolonged diabetic environment (high glucose and high insulin). Changes at the metabolite and peptide level were assessed by Liquid Chromatography–Mass Spectrometry (LC–MS2) and chemoinformatics. The results were integrated with published LC–MS2-based quantitative proteomics on the same in vitro model. Our findings were consistent with reports on other endothelial cell types and identified novel signatures of DNA/RNA, amino acid, peptide, and lipid metabolism in cells under a diabetic environment. Manual data inspection revealed disturbances on tryptophan catabolism and biosynthesis of phenylalanine-based, glutathione-based, and proline-based peptide metabolites. Fluorescence microscopy detected an increase in binucleation in cells under treatment that also occurred when human CAEC were used. This multi-omics study identified particular molecular perturbations in an induced diabetic environment that could help unravel the mechanisms underlying the development of cardiovascular disease in subjects with T2DM.
Parental competition for the regulators of chromatin dynamics in mouse zygotes
Authors: Ooga Masatoshi , Inoue Rei , Kazama Kousuke , Wakayama Sayaka , Kamimura Satoshi , Wakayama Teruhiko 
Published: July 2022

AbstractThe underlying mechanism for parental asymmetric chromatin dynamics is still unclear. To reveal this, we investigate chromatin dynamics in parthenogenetic, androgenic, and several types of male germ cells-fertilized zygotes. Here we illustrate that parental conflicting role mediates the regulation of chromatin dynamics. Sperm reduces chromatin dynamics in both parental pronuclei (PNs). During spermiogenesis, male germ cells acquire this reducing ability and its resistance. On the other hand, oocytes can increase chromatin dynamics. Notably, the oocytes-derived chromatin dynamics enhancing ability is dominant for the sperm-derived opposing one. This maternal enhancing ability is competed between parental pronuclei. Delayed fertilization timing is critical for this competition and compromises parental asymmetric chromatin dynamics and zygotic transcription. Together, parental competition for the maternal factor enhancing chromatin dynamics is a determinant to establish parental asymmetry, and paternal repressive effects have supporting roles to enhance asymmetry.
Carrier-envelope-phase measurement of sub-cycle UV pulses using angular photofragment distributions
Authors: Dong Xiao Xia , Liu Yan Rong , Kimberg Victor , Vendrell Oriol , Wu Yong , Wang Jian Guo , Chen Jing , Zhang Song Bin 
Published: July 2022

AbstractCarrier-envelope-phase (CEP) of sub-cycle ultraviolet (UV) pulse strongly influences the dynamics of quantum systems, but its characterization is not accessible experimentally. Here we investigate photodissociation of a diatomic molecule from its ground-rovibrational state in a linearly polarized weak sub-cycle UV pulse with a controlled CEP. The angular distribution of photofragments shows an asymmetric profile deviating from the well-known $${\cos }^{2}$$

cos

2

- or $${\sin }^{2}$$

sin

2

-like ones, which can be identified as a way to imprint CEP. We unveil that such an effect stems from the temporal neighboring rotational excitation by molecular permanent dipole interaction through the joint contributions between counter-rotating and rotating terms. This in turn, opens different pathways in photodissociation dynamics. Given that the temporal excitation between various states with close energies can be manipulated by CEP of sub-cycle UV pulses, our results pave ways for understanding and manipulating electron, nuclear and their joint dynamics with variation of CEP of attosecond pulses.
Synthetic Approaches to Diospongins: A Two Decade Journey
Authors: Tadiparthi Krishnaji , Chatterjee Sourav 
Published: July 2022

AbstractTetrahydropyran units having multiple stereogenic centers serve as excellent building blocks for various active pharmaceutical ingredients (APIs). In particular, the presence of the unique molecular architecture of the trisubstituted tetrahydropyran (THP) unit in diospongins enhances their biological activity due to multiple stereogenic centers and has attracted attention from the synthetic community over the last two decades. In this review, we discuss synthetic approaches to chiral and racemic forms of diospongins during the period 2006–2020 in chronological order.
Preoperative Chemoradiation versus Chemotherapy in Locally Advanced Resectable Esophageal Cancer: A Retrospective Study
Authors: Abbas Waseem , Pandit Archit , Goel Vineeta , Aggarwal Anjali , Acharya Rudra Prasad 
Published: July 2022

AbstractPreoperative chemotherapy and preoperative chemoradiation, both improve survival for locally advanced esophageal cancer proven in randomized trials and metanalysis. Limited data are available comparing these preoperative therapies especially in non-gastroesophageal junction squamous cell cancer of esophagus. In this retrospective analysis, 69 eligible patients of locally advanced esophageal cancer, who underwent preoperative chemoradiation followed by surgery or chemotherapy followed by surgery at our center were analyzed. The end points of study were overall survival, disease free survival, and histopathological response. Three weekly paclitaxel and carboplatin was used as neoadjuvant chemotherapy and weekly paclitaxel and carboplatin were used with radiation as per standard protocol. Median follow-up time was 35 months for surviving patients. Median overall survival was 44 months (95% CI 27.2–62.7) in chemoradiation group and it was not reached in the chemotherapy arm (p-value −0.832). The median disease-free survival for patients who underwent preoperative chemoradiation was 41 months and 34 months in preoperative chemotherapy group (p-value −0.812). Seven of 41 patients (17.1%) in chemoradiation group were circumferential resection margin (CRM) positive as compared with six of 28 (21.4%) in chemotherapy group (p = 0.650). A pathological complete response was seen in 13 (31.7%) patients in chemoradiation group and seven (21.4) patients in chemotherapy group. There was no survival advantage for preoperative chemoradiotherapy compared with preoperative chemotherapy in patients with predominant esophageal squamous cell carcinoma of locally advanced esophageal cancer. Despite the non-significant improvement from preoperative chemoradiation with respect to CRM positivity and pathological complete response rates, there was no difference in overall survival or disease-free survival.
The Impact of Anxiety and Depression on Academic Performance: A Cross-Sectional Study among Medical Students in Syria
Authors: Jamil Hasan , Alakkari Mohab , Al-Mahini Mohammed Saleem , Alsayid Muhammad , Al Jandali Omar 
Published: July 2022

Abstract
Background The National Medical Unified Examination (NMUE) is a milestone in the life of medical students in Syria. The selection for residency programs depends mainly on the NMUE score, where competitive specialties require higher scores. Therefore, preparation for the NMUE might be a source of anxiety and depression. This study aims at evaluating the impact of anxiety and depression on the NMUE score. A secondary objective is to determine the effect of some factors (i.e., exercise, having breakfast, adequate sleep, and social media) on anxiety and depression.
Methods A cross-sectional study was conducted using an online questionnaire and included medical students who were preparing for the October 2019 NMUE exam. The Generalized Anxiety Disorder scale (GAD-7) and the Patient Health Questionnaire (PHQ-9) were used to screen for anxiety and depression, respectively. NMUE scores were obtained from the official score report. Demographics and other potential confounding factors, such as Cumulative Grade Point Average, were obtained through the questionnaire.
Results One hundred and thirty (n = 130) students participated in the study, 83 of them were women (63.8%). The prevalence of anxiety and depression were 59.2 and 58%, respectively, with no difference between men and women. Both anxiety and depression were negatively correlated with the NMUE score. However, this relationship did not persist after controlling for other important predictors through multiple regression. Only exercising was statically significant in reducing PHQ-9 scores. None of the studied factors were significant in reducing GAD-7 scores.
Conclusion Although participants with higher anxiety/depression had lower NMUE scores, this association does not imply causation. The high prevalence of anxiety and depression (approximately two-thirds of the participants) is concerning and may pose a great threat to students' well-being and adversely affect the quality of care provided by them as future health care professionals.
Exploring a mobile-based language learning intervention to improve English language acquisition and acculturation among
migrants in Australia
Authors: Hebbani Aparna , Mersiades Michael , Deshmukh Ameya 
Published: July 2022

Abstract
This exploratory Australian study tested a novel mobile-based language learning service intervention (via
WhatsApp) called ‘Chatloop’ with 31 newly arrived migrants. This intervention was offered in tandem with the traditional Adult
Migrant English Program (AMEP) classroom curriculum. A mixed methods approach was employed to trial the intervention of Chatloop
with AMEP students at two Queensland TAFE campuses. Quantitative data were gathered to measure changes in participants’ English
proficiency, and qualitative interviews gathered narratives about the experience of using Chatloop. Chatloop intervention group
participants perceived it to be an interactive learning service that significantly improved their reading and writing skills,
acculturation, and enhanced social connections. Being able to access Chatloop on their mobile device 24/7 was deemed to be an
added advantage as compared to traditional classroom learning; it provided an opportunity to learn more about Australian culture
and communication patterns, at their convenience. These findings suggest that such mobile interventions can be a valuable means of
enhancing migrant’s English language learning and warrants future research.
From pen to movable type
Authors: Hammerschmidt Christopher , Adam Dominique Leite 
Published: July 2022

Abstract
Typography is an essential foundation for graphic design and information design. The teaching of this subject aims to set background knowledge on aspects such as the production and use of letterforms. In this article, we present an approach to teaching typography based on information design as an instrument for understanding and analyze results of students’ production of print posters during classes. We designed an integrative activity that sought to make them experience different facets of typography, from its relation with calligraphy to the sculptural nature of printing types and digital vector drawing on the computer, with an active learning approach.
A GLOBULAR CLUSTER TOWARD M87 WITH A RADIAL VELOCITY < − 1000 km s−1: THE FIRST HYPERVELOCITY CLUSTER
Authors: Caldwell Nelson , Strader Jay , Romanowsky Aaron J. , Brodie Jean P. , Moore Ben , Diemand Jurg , Martizzi Davide 
Published: July 2022

ABSTRACT
We report the discovery of an object near M87 in the Virgo Cluster with an extraordinary blueshift of −1025 km s−1, offset from the systemic velocity by >2300 km s−1. Evaluation of photometric and spectroscopic data provides strong evidence that this object is a distant massive globular cluster, which we call HVGC-1 in analogy to Galactic hypervelocity stars. We consider but disfavor more exotic interpretations, such as a system of stars bound to a recoiling black hole. The odds of observing an outlier as extreme as HVGC-1 in a virialized distribution of intracluster objects are small; it appears more likely that the cluster was (or is being) ejected from Virgo following a three-body interaction. The nature of the interaction is unclear, and could involve either a subhalo or a binary supermassive black hole at the center of M87.
Temperature dependence studies of tissue-mimicking phantoms for ultra-wideband microwave breast tumor detection
Authors: Slanina T , Nguyen D H , Moll J , Krozer V 
Published: July 2022

Abstract
Microwave imaging (MWI) systems are being investigated for breast cancer diagnostics as an alternative to conventional x-ray mammography and breast ultrasound. This work aims at a next generation of tissue-mimicking phantoms modelling the temperature-dependent dielectric properties of breast tissue over a large frequency bandwidth. Such phantoms can be used to develop a novel kind of MWI systems that exploit the temperature-dependent permittivity of tissue as a natural contrast agent. Due to the higher water content in tumor tissue, a temperature increase leads to a different change in the complex permittivity compared to surrounding tissue. This will generate a tumor dominated scattering response when the overall tissue temperature increases by a few degrees, e.g. through the use of microwave hyperthermia systems. In that case a differential diagnostic image can be calculated between microwave measurements at reference (around 37 °C) and elevated temperature conditions. This work proposes the design and characterization of agar-oil-glycerin phantoms for fatty, glandular, skin and tumor tissue. The characterization includes measurements with an open-ended coaxial probe and a network analyzer for the frequency range from 50 MHz to 20 GHz in a temperature-controlled environment covering the temperature range from 25 °C to 46 °C. The phantoms show an unique temperature response over the considered frequency bandwidth leading to significant changes in the real and imaginary part of the complex permittivity. Comparative studies with porcine skin and fat tissue show a qualitative agreement.
A-01 Changes in Fixational Eye Movements (FEMs) Following Concussion
Authors: Albrecht T J , Mehmel B M , Eagle S R , Leonard B T , Marchetti G F , Zhang M , Reecher H M , Snyder V , Holland C L , Rossi E A , Collins M W , Kontos A P 
Published: July 2022

Abstract
Purpose: Identify changes in fixational eye movement (FEMs) outcomes following concussion and assess the association of FEMs outcomes with clinical outcomes including symptoms, neurocognitive, and vestibular/ocular motor. Methods: Participants included thirty-three individuals (13–27 years old; 54.5% female) diagnosed with concussion within the past 21 days. Concussion diagnosis was determined using current consensus guidelines. A tracking scanning laser ophthalmoscope (TSLO) was used to measure FEMS. Participants completed ImPACT, PCSS, and VOMS. Within-subjects changes in FEMS outcomes were calculated using paired-samples t-tests. A backwards stepwise linear regression to predict days to clearance was utilized with all FEMS outcomes as predictors. Results: MeanDriftH (slope of cumulative drift regression calculated horizontally; mean difference = 0.04; p = 0.03; Cohen’s d = 0.43) and drift proportion significantly changed over time (mean difference = 0.03; p = 0.03, d = 0.44). Average peak velocity of microsaccade (MsPkVel) predicted days to recovery (R = 0.26; p = 0.045; n = 24). MsPkVel and average duration of blink correlated with select reports of nausea on VOMS. Microsaccade frequency (r = −0.36; p = 0.04) correlated with NPC fogginess. Drift amplitude (DriftAmp) correlated with NPC distance (r = −0.41; p = 0.02) and VMS headache (r = 0.35; p = 0.04). MeanDriftH was negatively correlated with verbal memory (r = −0.44; p = 0.01), whereas DriftAmp was positively correlated (r = 0.50; p = 0.003). Bivariate contour ellipse area was correlated (r = 0.43; p = 0.01) with slower reaction time on ImPACT. Conclusions: FEMs outcomes demonstrated significant change and were useful in predicting recovery time following concussion. FEMs measurements may provide brief, objective data to augment current clinical assessment and inform prognosis for recovery. Future researchers should examine FEMS outcomes from pre- to post-concussion and explore age and sex differences.