bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compression Therapy for HIV-Associated Kaposi Sarcoma Leg Lymphedema: Results of the Kenyan Improvised Compression for Kaposi Sarcoma Randomized Controlled Trial
Authors: Chang Aileen Y. , Karwa Rakhi , Odhiambo Haji , Were Phelix , Fletcher Sara L. , Tonui Edith C. , Kohn Michael A. , Lee Jeannette , Chang Di , Lensing Shelly , Namaemba Diana Flora , Busakhala Naftali , Kiprono Samson K. , Maurer Toby , Goodrich Suzanne , Pastakia Sonak D. 
Published: January 2022

PURPOSE Evaluate the effectiveness of compression while receiving chemotherapy compared with chemotherapy alone in the treatment of HIV-associated Kaposi sarcoma (KS) lymphedema. METHODS A randomized controlled trial was conducted in a single oncology clinic in western Kenya ( NCT03404297 ). A computer-generated randomization schedule was used to allocate treatment arms. Randomized block design was used for stratification by lymphedema stage. Participants were HIV positive adults age ≥ 18 years on antiretroviral therapy with biopsy-proven KS associated with leg lymphedema and being initiated on chemotherapy. The intervention was 10 weeks of weekly clinic-based application of two-component paste compression bandages. The primary outcome was change in the Lower Extremity Lymphedema Index (LELI) score from week 0 to week 14. The secondary outcomes were change in the Lymphedema Quality of Life measure (LYMQOL) and change in the European Organisation for Research and Treatment of Cancer (EORTC) QLQ-C30 score from week 0 to week 14. Blinded outcome assessments were conducted. RESULTS Of 30 participants randomly assigned, 25 eligible patients (chemotherapy [control], n = 13; compression plus chemotherapy [intervention], n = 12) returned at week 14. Change in LELI, LYMQOL, and EORTC QLQ-C30 scores between week 14 and week 0 did not significantly differ by arm. The mean (standard deviation) change in LELI score was –25.9 (34.6) for the control arm compared with –13.3 (29.5) for the intervention arm, P = .340. The difference (95% CI) in the change in LELI score was –12.6 (–39.3 to 14.1). CONCLUSION Future studies evaluating a 14-week change in LELI for KS lymphedema should assume a standard deviation of approximately 30. Lessons learned from this pilot trial should inform the development of a larger, multicenter trial to evaluate the effectiveness of compression for KS lymphedema.
Universal Germline Panel Testing for Individuals With Pheochromocytoma and Paraganglioma Produces High Diagnostic Yield
Authors: Horton Carolyn , LaDuca Holly , Deckman Ashley , Durda Kate , Jackson Michelle , Richardson Marcy E , Tian Yuan , Yussuf Amal , Jasperson Kory , Else Tobias 
Published: January 2022

Abstract

Background
Practice guidelines to identify individuals with hereditary pheochromocytomas and paragangliomas (PPGLs) advocate for sequential gene testing strategy guided by specific clinical features and predate the routine use of multigene panel testing (MGPT).

Objective
To describe results of MGPT for hereditary PPGL in a clinically and ancestrally diverse cohort.

Setting
Commercial laboratory based in the United States.

Methods
Clinical data and test results were retrospectively reviewed in 1727 individuals who had targeted MGPT from August 2013 through December 2019 because of a suspicion of hereditary PPGL.

Results
Overall, 27.5% of individuals had a pathogenic or likely pathogenic variant (PV), 9.0% had a variant of uncertain significance, and 63.1% had a negative result. Most PVs were identified in SDHB (40.4%), followed by SDHD (21.1%), SDHA (10.1%), VHL (7.8%), SDHC (6.7%), RET (3.7%), and MAX (3.6%). PVs in FH, MEN1, NF1, SDHAF2, and TMEM127 collectively accounted for 6.5% of PVs. Clinical predictors of a PV included extra-adrenal location, early age of onset, multiple tumors, and positive family history of PPGL. Individuals with extra-adrenal PGL and a positive family history were the most likely to have a PV (85.9%). Restricting genetic testing to SDHB/C/D misses one-third (32.8%) of individuals with PVs.

Conclusion
Our data demonstrate a high diagnostic yield in individuals with and without established risk factors, a low inconclusive result rate, and a substantial contribution to diagnostic yield from rare genes. These findings support universal testing of all individuals with PPGL and the use of concurrent MGPT as the ideal platform.
A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease
Authors: Li Zhao-Feng , Cui Lei , Jin Mi-Mi , Hu Dong-Yan , Hou Xiao-Gang , Liu Shu-Shu , Zhang Xiong , Zhu Jian-Hong 
Published: January 2022

ABSTRACT
Parkinson's disease (PD) is associated with α-synuclein-based Lewy body pathology, which has been difficult to observe in conventional two-dimensional (2D) cell culture and even in animal models. We herein aimed to develop a three-dimensional (3D) cellular model of PD to recapitulate the α-synuclein pathologies. All-trans-retinoic acid-differentiated human SH-SY5Y cells and Matrigel were optimized for 3D construction. The 3D cultured cells displayed higher tyrosine hydroxylase expression than 2D cells and improved dopaminergic-like phenotypes, as suggested by RNA-sequencing analyses. Multiple forms of α-synuclein, including monomer, and low- and high-molecular mass oligomers, were differentially present in the 2D and 3D cells, but mostly remained unchanged upon N-methyl-4-phenyl pyridine or rotenone treatment. Phosphorylated α-synuclein was accumulated, and detergent-insoluble α-synuclein fraction was observed, in the neurotoxin-treated 3D cells. Importantly, Lewy body-like inclusions were captured in the 3D system, including proteinase K-resistant α-synuclein aggregates, ubiquitin aggregation, and β-amyloid and β-sheet protein deposition. The study provides a unique and convenient 3D model of PD that recapitulates critical α-synuclein pathologies and should be useful in multiple PD-associated applications.
Diverse mechanisms regulate contractile ring assembly for cytokinesis in the two-cell Caenorhabditis elegans embryo
Authors: Ozugergin Imge , Mastronardi Karina , Law Chris , Piekny Alisa 
Published: January 2022

ABSTRACT
Cytokinesis occurs at the end of mitosis as a result of the ingression of a contractile ring that cleaves the daughter cells. The core machinery regulating this crucial process is conserved among metazoans. Multiple pathways control ring assembly, but their contribution in different cell types is not known. We found that in the Caenorhabditis elegans embryo, AB and P1 cells fated to be somatic tissue and germline, respectively, have different cytokinesis kinetics supported by distinct myosin levels and organization. Through perturbation of RhoA or polarity regulators and the generation of tetraploid strains, we found that ring assembly is controlled by multiple fate-dependent factors that include myosin levels, and mechanisms that respond to cell size. Active Ran coordinates ring position with the segregating chromatids in HeLa cells by forming an inverse gradient with importins that control the cortical recruitment of anillin. We found that the Ran pathway regulates anillin in AB cells but functions differently in P1 cells. We propose that ring assembly delays in P1 cells caused by low myosin and Ran signaling coordinate the timing of ring closure with their somatic neighbors.
This article has an associated First Person interview with the first author of the paper.
Label2label: training a neural network to selectively restore cellular structures in fluorescence microscopy
Authors: Kölln Lisa Sophie , Salem Omar , Valli Jessica , Hansen Carsten Gram , McConnell Gail 
Published: January 2022

ABSTRACT
Immunofluorescence microscopy is routinely used to visualise the spatial distribution of proteins that dictates their cellular function. However, unspecific antibody binding often results in high cytosolic background signals, decreasing the image contrast of a target structure. Recently, convolutional neural networks (CNNs) were successfully employed for image restoration in immunofluorescence microscopy, but current methods cannot correct for those background signals. We report a new method that trains a CNN to reduce unspecific signals in immunofluorescence images; we name this method label2label (L2L). In L2L, a CNN is trained with image pairs of two non-identical labels that target the same cellular structure. We show that after L2L training a network predicts images with significantly increased contrast of a target structure, which is further improved after implementing a multiscale structural similarity loss function. Here, our results suggest that sample differences in the training data decrease hallucination effects that are observed with other methods. We further assess the performance of a cycle generative adversarial network, and show that a CNN can be trained to separate structures in superposed immunofluorescence images of two targets.
Underweight and associated factors among children under five years in North Shewa Zone, Oromia, Ethiopia: An observational community-based study
Authors: Yazew Tamiru 
Published: January 2022

Background: Acute and chronic child undernutrition is a continuous problem in Ethiopia. Therefore, this study was initiated to compare the prevalence of underweight and its associated factors among children aged 6-23 months in the Kuyu district, North Shewa zone, Oromia, Ethiopia. Methods: An observational community-based study was conducted on 612 children (304 from household security and 308 from household food insecurity). A structured and standardize questionnaire was used in this study. Anthropometric measurements were generated using WHO standardize. Data was analyzed using SPSS version 20.0. Bivariate and multivariate logistic regression analysis was used to identify the independent variables associated with underweight (weight-for-age) among children in household food security and insecurity, a p value less than 0.05 with 95%CI was considered as statistically significant. Results: The results indicated that 30.9% [95%CI; 25.7, 36.2] and 36.7% [95% CI; 31.8, 42.5] of children were underweight for their age in household food security and insecurity. Low wealth status (AOR=3.2; 95%CI: 1.099, 9.275), poor dietary diets (AOR=5.2; 95%CI: 2.046, 13.27), and lack of breastfeeding for two years (AOR= 2.1; 95%CI= 1.78, 5, 42) were associated with underweight children in household food security. Whereas lack of antenatal care visits (AOR=0.52; 95%CI: 0.12, 0.68) and poor dietary diets (AOR=3.01; 95%CI= 2.1, 17.4) were other independent variables associated with underweight children in household food insecurity. Conclusions: This study established that there was a high prevalence of underweight in children from Oromia. Therefore, introducing household income generating activities are vital interventions in order to overcome the problem of undernutrition in this region.
Case Report: Digital analysis of occlusion with T-Scan Novus in occlusal splint treatment for a patient with bruxism
Authors: Shopova Dobromira , Bozhkova Tanya , Yordanova Svetlana , Yordanova Miroslava 
Published: January 2022

Bruxism is a disease with a multifactorial etiology. Its clinical manifestations are most often an unaesthetic smile with abraded tooth surfaces, temporomandibular disorders and muscle hyperactivity. Here we present a case of bruxism where proper articulation of the occlusal splint was performed using the T-scan Novus system. A patient with bruxism underwent treatment with stabilization splint made by 3D printer technology. Intraoral scanning was performed using Trios Color (3Shape, 2014), and the digital design was achieved using the 3Shape Dental system design - splint studio. Formlabs Form 2 printer with biocompatible resin Dental LT Clear Resin was used for printing. The T-Scan Novus system with software attached to it, version 9.1, was used for digital examination of the occlusion. A 2.7 mm thick occlusal splint was developed, and the software adapted the occlusion with antagonists. After adjustment with T-Scan Novus, a reduction in disocclusion time of the patient was achieved, which is a desired result in the treatment of bruxism. The position of the joint components was proven radiologically. The treatment of bruxism with splint therapy continues to be the main method of treatment. Using digital technology allows for more accurate constructions and precise balancing of occlusal relationships.
Demand Estimation Using Managerial Responses to Automated Price Recommendations
Authors: Garcia Daniel , Tolvanen Juha , Wagner Alexander K. 
Published: January 2022

We provide a new framework to identify demand elasticities in markets where managers rely on algorithmic recommendations for price setting and apply it to a data set containing bookings for a sample of midsized hotels in Europe. Using nonbinding algorithmic price recommendations and observed delay in price adjustments by decision makers, we demonstrate that a control-function approach, combined with state-of-the-art model-selection techniques, can be used to isolate exogenous price variation and identify demand elasticities across hotel room types and over time. We confirm these elasticity estimates with a difference-in-differences approach that leverages the same delays in price adjustments by decision makers. However, the difference-in-differences estimates are more noisy and only yield consistent estimates if data are pooled across hotels. We then apply our control-function approach to two classic questions in the dynamic pricing literature: the evolution of price elasticity of demand over and the effects of a transitory price change on future demand due to the presence of strategic buyers. Finally, we discuss how our empirical framework can be applied directly to other decision-making situations in which recommendation systems are used. This paper was accepted by Omar Besbes, revenue management and market analytics. Funding: D. Garcia acknowledges financial support from the Austrian Science Fund [Single Project “Understanding Consumer Search” FWF-P 30922]. Supplemental Material: The online appendix and data are available at https://doi.org/10.1287/mnsc.2021.4261 .
External Interfaces or Internal Processes? Market Positioning and Divergent Professionalization Paths in Young Ventures
Authors: DeSantola Alicia , Gulati Ranjay , Zhelyazkov Pavel I. 
Published: January 2022

We explore how the initial market positioning of entrepreneurial ventures shapes how they professionalize over time, focusing specifically on the development of functional roles. In contrast to existing literature, which presumes a uniform march toward professionalization as ventures scale and complete developmental milestones, we advance a contingent perspective, distinguishing between the development of external interface functions (marketing & sales and customer development) and internal process functions (accounting, human resources, and finance). Specifically, we argue that positioning in an unconventional market space raises demand for external engagement that focuses ventures’ attention and resources toward developing external interface roles. At the same time, such unconventional ventures are less apt to elaborate their internal process roles relative to more conventional peers. We test these predictions using a novel longitudinal data set on the internal organizations of 3,748 U.S.-based entrepreneurial ventures. In contrast to common assumptions of convergent professionalization, our theory and findings advance the perspective that ventures pursue divergent professionalization paths based on their initial market positioning as they scale up. Funding: This research was generously funded in part by a Ewing Marion Kauffman Foundation Dissertation Fellowship awarded to the first author. Supplemental Material: The online appendix is available at https://doi.org/10.1287/orsc.2021.1561 .
Investigation on the Effect of Impeller Design Parameters on Performance of a Low Specific Speed Centrifugal Pump Using Taguchi Optimization Method
Authors: Ayremlouzadeh Hadi , Jafarmadar Samad , Amini Niaki Seyed Reza 
Published: January 2022

In order to investigate the effect of blade design on pump performance, a CFD analysis was carried out, and the results were compared with experimental performance data of a low specific speed radial pump, which presents a good agreement. After model verification, the effect of impeller geometrical parameters includes blade outlet angle, wrap angle, and width at the exit, was investigated on the pump’s performance. Moreover, these parameters were chosen on three levels using an L9 orthogonal standard array of the Taguchi optimization method. The efficient levels of variables were calculated using the analysis of variance (ANOVA) method. The results revealed that impeller width at exit and blade outlet angle is the most effective pump shaft power and efficiency parameters. To minimize power, the optimal levels are the outlet angle of 27∘∘, wrap angle of 150∘∘, and width at the exit of 9 mm. Further, an outlet angle of 23∘∘, a wrap angle of 155∘∘, and a width at the exit of 9 mm lead to maximum pump efficiency. According to the validation simulations, an increase of 2.4% inefficiency and a minimum power of 3.9KW were achieved. The Overall Evaluation Criteria (OEC) technique revealed that considering 23∘∘, 160∘∘, and 9 mm for outlet angle, wrap angle, and width at the exit, minimum shaft power, and maximum pump efficiency will be achieved. ANOVA introduced width at the exit as the most governing parameter of pump performance characteristics.
Balance y estado de la judicialización protectora como respuesta a la violencia estatal en contra de niños y niñas mapuche en 2020
Authors: Corvalán Cristopher , Molina Claudia , Nahuelcheo Pamela , Saavedra Sebastián , Vargas Ruth 
Published: January 2022

El presente artículo pretende visibilizar y reflexionar sobre las multiples violaciones de derechos humanos de que son víctimas los niños y niñas mapuche en contexto de pandemia en Wallmapu (sur de Chile) marcado por un estado de excepción (2020). Para tal efecto se recurrira a la estrategia de estudio de casos, lo que permitira delimitar y describir tres ámbitos del derecho en el que transcurren las estrategias jurídicas emancipatorias que buscan reparar, asegurar o ampliar los ámbitos de protección de los derechos de la niñez mapuche. Se focaliza el analisis al ámbito de los tribunales superiores de justicia a través de las acciones constitucionales de protección y amparo; al ámbito del derecho penal con el análisis de casos de niños y adolescentes mapuche vulnerados por acciones de agentes del Estado; y al ámbito del derecho de familia analizando la acción de un grupo de magistradas frente a la vulneración de derechos de niñez. Se concluye sobre los incipientes avances en materia de la protección de NNA mapuche y las dificultades para las cortes y tribunales en reconocer las violaciones de sus derechos, brindar protección o identificar y condenar los responsables.
Sources and Methodologies for Micro, Small and Medium Enterprises Study in Brazil
Authors: Dalla Costa Armando João , Alam Naijla Alves El 
Published: January 2022

This article uncovers relevant sources and methodologies to gather knowledge about micro, small and medium enterprises (MSME) in Brazil. To achieve its purpose, the text presents sources and methodologies. This type of study is relatively neglected concerning MSMEs. Until this moment, not much data has been reported about these companies’ categories, and even less in advanced research. Among the most significant data and methodology of addressing MSMEs in the country, the text highlights the surveys conducted by the Brazilian Institute of Geography and Statistics (IBGE), the Brazilian Micro and Small Business Support Service (SEBRAE), and the international annual research Global Entrepreneurship Monitor (GEM) a study on the entrepreneurial activity, which Brazil is part for more than 20 years. The Brazilian institutions, as well as consulting for those companies, collect key data that posteriorly become research sources. Also, when explaining how institutions chose and treated the data, they own specific methodologies regarding those businesses in Brazil. The paper also points out the importance of constant law monitoring, adjustment, and technology incentives. This article's contribution is to present a research methodology on MSME in Brazil with relevant data.
Low-dose aspirin and incidence of lung carcinoma in patients with chronic obstructive pulmonary disease in Hong Kong: A cohort study
Authors: Yu Si-Yeung , Ip Mary Sau-Man , Li Xue , Cheung Ka-Shing , Ren Qing-Wen , Wu Mei-Zhen , Li Hang-Long , Wong Pui-Fai , Tse Hung-Fat , Yiu Kai-Hang 
Published: January 2022

Background
Evidence suggests that chronic obstructive pulmonary disease (COPD) is associated with a higher risk of lung carcinoma. Using a territory-wide clinical electronic medical records system, we investigated the association between low-dose aspirin use (≤160 mg) among patients with COPD and incidence of lung carcinoma and the corresponding risk of bleeding.

Methods and findings
This is a retrospective cohort study conducted utilizing Clinical Data Analysis Reporting System (CDARS), a territory-wide database developed by the Hong Kong Hospital Authority. Inverse probability of treatment weighting (IPTW) was used to balance baseline covariates between aspirin nonusers (35,049 patients) with new aspirin users (7,679 patients) among all eligible COPD patients from 2005 to 2018 attending any public hospitals. The median age of the cohort was 75.7 years (SD = 11.5), and 80.3% were male. Competing risk regression with Cox proportional hazards model were performed to estimate the subdistribution hazard ratio (SHR) of lung carcinoma with low-dose aspirin and the associated bleeding events. Of all eligible patients, 1,779 (4.2%, 1,526 and 253 among nonusers and users) were diagnosed with lung carcinoma over a median follow-up period of 2.6 years (interquartile range [IQR]: 1.4 to 4.8). Aspirin use was associated with a 25% lower risk of lung carcinoma (SHR = 0.75, 95% confidence interval [CI] 0.65 to 0.87, p = <0.001) and 26% decrease in lung carcinoma–related mortality (SHR = 0.74, 95% CI 0.64 to 0.86, p = <0.001). Subgroup analysis revealed that aspirin was beneficial for patients aged above or below 75 years, but was also beneficial among populations who were male, nondiabetic, and nonhypertensive. Aspirin use was not associated with an increased risk of upper gastrointestinal bleeding (UGIB) (SHR = 1.19, 95% CI 0.94 to 1.53, p = 0.16), but was associated with an increased risk of hemoptysis (SHR = 1.96, 95% CI 1.73 to 2.23, p < 0.001). The main limitations of the study were (i) that one group of patients may be more likely to seek additional medical attention, although this was partially mitigated by the use of propensity score analysis; and (ii) the observational nature of the study renders it unable to establish causality between aspirin use and lung carcinoma incidence.

Conclusions
In this study, we observed that low-dose aspirin use was associated with a lower risk of lung carcinoma and lung carcinoma–related mortality among COPD patients. While aspirin was not associated with an increased risk of UGIB, the risk of hemoptysis was elevated.