bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional insights from targeted imaging BACE1: the first near-infrared fluorescent probe for Alzheimer’s disease diagnosis
Authors: Bi Anyao , Wu Junyong , Huang Shuai , Li Yongjiang , Zheng Fan , Ding Jipeng , Dong Jie , Xiang Daxiong , Zeng Wenbin 
Published: December 2022

Abstract

Background
β-Secretase (BACE1) is the vital enzyme in the pathogenic processes of Alzheimer's disease (AD). However, the development of a powerful tool with sensitivity for BACE1 determination in vivo is a challenge.

Methods
A novel NIR fluorescent probe HBAE was synthetized from 2-hydroxy-3-methylbenzaldehyde and 2-amino-benzenethiol by 5 steps. The fluorescence mechanism in the ESIPT systems of HBAE probe was insighted with time-dependent density functional theory (TD-DFT) at the TDPBE0 level with the def2-TZVP approach. The corresponding docking between HBAE and BACE1 (PDB: 5I3Y) was performed through the ducking method by DOCK6.8. Then the BBB permeability of HBAE is verified by transwell orifice plate. 22-month-old male AD-model (5XFAD) mice and age-matched wild-type mice were employed to observe the brain kinetics by intravenous injection. Finally, Immunohistochemistry was performed on the AD brain section to reveal the levels of BACE1 in hippocampus and cortex areas and other regions in AD mice through the brain tissue slices by HBAE.

Results
The NIR fluorescent probe HBAE was successfully applied in imaging BACE1 in AD model mice. The capability of HBAE in reflecting different level of BACE1 was performed by the specific imaging of the hippocampus region.

Conclusions
We reported the first ESIPT near-infrared fluorescence probe HBAE for monitoring endogenous BACE1 in the AD live model mice, thus offering a versatile chemical tool for visualizing in the pathological processes of AD live brains. Remarkably, high resolution images showed the localization of red fluorescence stains in hippocampus of the AD brain. This study provides a promising way for functional insights from protein BACE1 in vivo.
Effective strategies against COVID-19 and the importance of infection sequelae
Authors: Khalife Jade 
Published: December 2022

AbstractCOVID-19 is a serious threat to human health and development. The acute burden of the pandemic includes more than 18.2 million deaths worldwide, and is unprecedented in modern times. This represents only a fraction of the total burden, as it excludes infection sequelae. An effective global strategic paradigm has been missing throughout the pandemic. The ‘flattening the curve’ approach neglected the importance of infection sequelae, and being centered on healthcare capacity was conceptually contrary to a people-centered health system. In March 2022, the World Health Organization revised its pandemic approach, importantly shifting emphasis away from managing transmission and towards prevention. Despite limitations, this now recognizes the role of infection sequelae, whose impact is becoming clearer in both variety and scale. Drawing on the foundational concepts of Sun Tzu and Carl von Clausewitz, most country approaches do not qualify as strategies, but rather as operational plans. They are also largely ineffective, neglecting infection sequelae, viral evolution dangers and other parameters. The purpose of this article is to summarize the evidence on COVID-19 infection sequelae, and alongside other contextual parameters use this to motivate that infection should be prevented. This is then used to answer the question: What is an effective strategy against COVID-19?
Peripheral facial nerve palsy in children: clinical manifestations, treatment and prognosis
Authors: Bilge Serap , Mert Gülen Gül , Hergüner M. Özlem , İncecik Faruk , Sürmelioğlu Özgür , Bilen Sevcan , Yılmaz Levent 
Published: December 2022

Abstract
Background
Sudden onset of unilateral weakness of the upper and lower muscles of one side of the face is defined as peripheral facial nerve palsy. Peripheral facial nerve palsy is often idiopathic and sometimes it could be due to infectious, traumatic, neoplastic, and immune causes. This study aimed to report the clinical manifestation, evaluation, and prognosis in children with peripheral facial nerve palsy.

Methods
57 children under 18 years of age diagnosed with peripheral facial nerve palsy at Çukurova University, Balcalı Hospital, between January 2018 and September 2021, were included in the study.

Results
The mean age of the children at the time of diagnosis was 9.6 ± 7, 4 years. Thirty-two (56.1%) of the patients were female and 25 (43.9%) were male. A total of 57 patients were diagnosed with peripheral facial nerve palsy and categorized into many groups by etiology: idiopathic Bell’s palsy in 27 (47.5%), infectious in 11 (19.2%), traumatic in 6 (10.5%), and others (due to congenital, immune, neoplastic, Melkersson–Rosenthal syndrome, drug toxicity, and iatrogenic causes) in 13 (22.8%). Forty-six of the children achieved full recovery under oral steroids within 1–7 months. Four patients with acute leukemia, myelodysplastic syndrome, Mobius syndrome and trauma did not recover and two patients (schwannoma, trauma) showed partial improvement. Five patients could not come to follow-up control.

Conclusion
Peripheral facial nerve palsy is a rare condition in children with different causes. It could be idiopathic, congenital, or due to infectious, traumatic, neoplastic, and immune reasons. So, when a child presents with facial palsy, a complete clinical history and a detailed clinical examination are recommended. Giving attention to the red flag is very important. Peripheral facial nerve palsy in children is considered to have a good prognosis.
Fracture resistance and flexural strength of endodontically treated teeth restored by different short fiber resin composites: a preclinical study
Authors: Gamal Wael , Abdou Ahmed , Salem Ghada A. 
Published: December 2022

Abstract
Aim
To evaluate the effect of using different short fiber-reinforced resin composites on fracture resistance and flexural strength of endodontically treated tooth and compare it with nano-filled resin composite.

Methods
Sixty human premolars were used for the fracture resistance test. Root canals were sequentially enlarged using a Pro-taper system from SX to F3 and obturated with Gutta-percha. Mesio-Occluso-Distal (MOD) cavities were prepared in all teeth. Teeth were then divided into 3 groups (n = 20 each) based on the type of resin composite. Group 1: Alert fiber-reinforced resin composite, Group 2: EverX Flow fiber-reinforced resin composite, and Group 3: Z350 nano-filled resin composite. Each group were subjected to a load till fracture using a universal testing machine to measure the fracture resistance. For the flexural strength test, 10 specimens from each material were prepared and 3- point bending tests were performed. The results of both tests were analyzed by using Weibull analysis.

Results
Teeth restored with Ever X fiber-reinforced resin composite conveyed the highest significant fracture resistance and flexural strength value when compared to the other two materials.

Conclusions
Short Fiber-reinforced resin composite can be considered a better choice for the restoration of MOD cavities in endodontically treated teeth.
Prognostic markers of post-stroke depression (PROMoSD): study protocol of a prospective single-center observational study on raphe hypoechogenicity as a predictor of post-stroke depression
Authors: Richter Daniel , Ebert Andreas , Mazul-Wach Lisa , Ruland Quirin , Charles-James Jeyanthan , Gold Ralf , Tsivgoulis Georgios , Juckel Georg , Krogias Christos 
Published: December 2022

Abstract
Introduction
Post-stroke depression (PSD) is an important and frequent non-motor complication after a stroke. As valid prediction of PSD occurrence is still not possible, the unselective use of preventive therapy in stroke patients has risen a questionable risk-to-benefit consideration. Therefore, there is a need to increase the prediction probability of PSD to identify patients at very high risk of a depressive complication who might benefit from preventive therapy. In this context, a brainstem raphe hypoechogenicity (BRH) in transcranial sonography (TCS) has previously been associated with depressive symptoms in a broad spectrum of diseases. BRH might therefore represent a valid maker of vulnerability for depressive symptoms that could be of interest in the risk assessment of PSD occurrence.

Methods
In the prognostic markers of post-stroke depression (PROMoSD) study, a prospective, observational, single-center, investigator-initiated study, we aim to include 100 patients with acute ischemic stroke (AIS). Besides data on clinical characteristics and baseline psychiatric assessment, we conduct a TCS examination to identify patients with BRH. The primary outcome is the incidence of PSD three months after inclusion, determined by a blinded investigator according to the fifth version of the Diagnostic and Statistical Manual of Mental Disorders (DSM-V) criteria.

Perspective
The results of PROMoSD will answer the question of whether screening of BRH after AIS improves the prediction of PSD occurrence. A positive result of this study could have direct consequences on psychiatric support after AIS by streamlining diagnostic and therapeutic algorithms.

Trial registration ClinicalTrials.gov identifier no. NCT05580198.
Clinical characterization of 72 patients with del(22)(q11.2q11.2) from different ethnic backgrounds
Authors: Méndez-Rosado Luis A. , de León-Ojeda Norma , García Alina , Sheth Frenny , Gaadi Asmaa , Bousfiha Ahmed Aziz , Lehlimi Mouna , Natiq Abdelhafid , Kurinnaia Oxana S. , Vorsanova Svetlana G. , Iourov Ivan , Huhle Dagmar , Liehr Thomas 
Published: December 2022

Abstract
Background
DiGeorge syndrome (DGS), caused by a deletion del(22)(q11.2q11.2), is the most frequently observed microdeletion syndrome. There is a vast clinical heterogeneity in DGS, and several studies suggested also heterogeneity of clinical signs and phenotypic appearance to be related to ethnic differences. Here, clinical characteristics of 72 patients with molecular diagnosed deletion del(22)(q11.2q11.2) derived from different countries from Europe, America, Africa, and Asia are summarized and compared.

Results
Unless ethnic differences, the expected major clinical signs were present in all cases. Frequent clinical manifestations found in this study were congenital heart disease with 68% (49/72), followed by dysmorphic features found in 61% (44/72); neurodevelopmental disorders were present in 43% (31/72) and thymus hypoplasia/aplasia in 32% (23/72). However, clinical features of the patients appeared/were recognized at different times during their lives. Within the group, under 2 years predominated heart disease, dysmorphic features, and hypocalcemia and/or hypoparathyroidism. In the group older than 2 years, the following combination of clinical findings was most frequent: dysmorphic features, congenital heart disease, intellectual disability, and immunological disorders. In the eight cases detected prenatally, abnormal sonographic findings were the major clinical signs (cardiovascular malformations and renal malformations).

Conclusions
Despite the heterogeneous nature of the sample analyzed, a number of clinical findings could be highlighted to be useful for the clinical delineation of this DGS. Interestingly, diagnostic indicators may vary depending on the age at diagnosis. Finally, apparent differences in DGS patients from different regions seem to be rather due to applied test systems than to real differences in patients from different ethnicities.
A rare case report of endovascular management of pediatric spinal arteriovenous malformation
Authors: Amaravadi Srikanth , Bhanu Kovilapu Uday , Maheshwari Saurabh , Dhagat Peeyush , Pandey Shweta , Menon Sumesh 
Published: December 2022

Abstract
Background
Spinal arteriovenous malformations (AVM) consist of a heterogeneous group of pathological vascular entities that affect the spinal cord parenchyma either directly or indirectly.

Case presentation
We present an unusual case of spinal arteriovenous malformation (type 2) in an 18-month-old girl who presented with weakness of both lower limbs and urinary incontinence. She was diagnosed with a spinal AVM with large intramedullary nidus and paraspinal extension which was managed with endovascular embolization.

Conclusion
Spinal cord AVM in children can be debilitating. If presented early, patient can be taken up for embolization, which is a relatively safe procedure with better neurological outcome. Our case illustrates the successful role of interventional radiology in the treatment of this rare condition.
Cytotoxic effects of Trachyspermum ammi and Ferula assafoetida on MCF-7 and MDA-MB-468 breast cancer cell lines
Authors: Moulazadeh Alireza , Ranjbar Razieh , Dakhili Ardestani Amin , Ranjbar Kamran , Farjadfar Akbar , Kouhpayeh Seyyed Amin , Abdollahi Abbas , Najafipour Sohrab 
Published: December 2022

Abstract
Background
The induction of oxidative stress is one of the most important cancer etiologies. Plant essential oils contain many effective antioxidant compounds in improving oxidative stress. In the present study, the pharmacological potential of Trachyspermum ammi essential oil (TAEO) and Ferula assafoetida essential oil (FAEO) was compared in oxidative stress improvement and cytotoxic effect. TAEO and FAEO were prepared by Clevenger apparatus, and the medicinal compounds in the essential oils were evaluated by GC–MS assay. The TAEO and FAEO were also evaluated as to their phenolic and flavonoid content, monovalent reducing power, and total radical scavenging activity, respectively, by Folin–Ciocalteu, aluminum chloride, FRAP, and DPPH methods. The cytotoxic effect of the TAEO and FAEO was evaluated by MTT assay on MCF-7 (ER+) and MDA-MB-468 (ER−) breast cancer cell lines.

Results
The GC–MS analysis indicated that thymol and (E)-Sec-Butyl propenyl disulfide, respectively, were the highest components of TAEO and FAEO. The phenolic content (P < 0.0001), flavonoid content (P < 0.0001), reducing power (P < 0.0001), radical scavenging activity (P < 0.0001), and cytotoxic effect (P < 0.05) of TAEO were significantly higher than FAEO. The IC50 value of the cytotoxic effect of TAEO on MCF-7 and MDA-MB-468 after 72 h of incubation was, respectively, 192.5 ± 42.57 and 331.4 ± 7.24 ppm.

Conclusion
The cytotoxic effect of TAEO was more potent on the MCF-7 cell line, probably in an estrogen-dependent manner of cellular growth inhibition. It appears that TAEO has a high capacity for improving oxidative stress and inhibiting cell proliferation in breast cancer.
Dysregulation of intraovarian redox status and steroidogenesis pathway in letrozole-induced PCOS rat model: a possible modulatory role of l-Carnitine
Authors: Elmosalamy Sherif H. , Elleithy Ebtihal M. M. , Ahmed Zainab Sabry Othman , Rashad Maha M. , Ali Ghada E. , Hassan Neven H. 
Published: December 2022

Abstract
Background
Polycystic ovarian syndrome (PCOS) is a reproductive disorder associated with several endocrine and metabolic alterations. The mechanism underlying this syndrome is controversial. On the other hand, drugs used for the treatment are associated with several side effects and poor in controlling PCOS phenotype. l-Carnitine (LC) has been reported to have a significant regulatory function on the redox and metabolic status of female reproductive system. Nevertheless, its regulatory pathways to regulate PCOS are still under investigation. Therefore, this study aimed to evaluate the effects of LC on the steroidogenic pathways, oxidative stress markers and metabolic profile in letrozole (LTZ)-induced PCOS rat model.

Methods
For this aim, animals were divided into four groups (n = 6). Control group, untreated letrozole-induced PCOS group (1 mg/kg bwt) for 21 days, PCOS group treated with l-Carnitine (100 mg/kg bwt) for 14 days and PCOS group treated with clomiphene citrate (2 mg/kg bwt) for 14 days. Finally, body and ovarian weight, metabolic state(glucose and lipid profile), hormonal assays (testosterone, 17 β estradiol, LH and FSH levels), intraovarian relative gene expression (CYP17A1, StAR, CYP11A1 and CYP19A1 genes), ovarian redox state (malondialdehyde (MDA), reduced glutathione content (GSH) and catalase enzyme activity (CAT)) as well as serum total antioxidant capacity (TAC) were detected. Also, histomorphometric ovarian evaluation (number and diameter of cystic follicles, granulosa cell thickness and theca cell thickness) as well as immune expression of caspase-3 of granulosa cells of cystic follicles were determined.

Results
LC significantly improved ovarian redox state (GSH, MDA and CAT), steroidogenic pathways gene expression (CYP17A1, StAR, CYP11A1 and CYP19A1 genes), hormonal profile (Follicle stimulating hormone (FSH) and luteinizing hormone (LH), testosterone and estradiol), metabolic state (Glucose and lipid profile) histomorphometric alterations and decreased caspase 3 immune reaction of granulosa cells.

Conclusion
l-Carnitine supplementation can ameliorate the PCOS phenotype through its energetic, antioxidant and antiapoptotic functions as well as steroidogenesis regulatory role. This protocol could be modified to produce the best therapeutic benefits, and it could be regarded as a prospective therapeutic intervention for PCOS.
Traditional and novel diagnostic biomarkers for acute myocardial infarction
Authors: Khalil Husam 
Published: December 2022

Abstract
Background
Acute myocardial infarction (AMI) is a leading cause of death worldwide. The first hours of acute myocardial infarction are correlated with the highest risk of death. Therefore, early diagnosis of the infarction seriously affects the efficacy of the treatment administered to the patient. Misdiagnosing patients with chest pain often leads to inappropriate admission of them as acute myocardial infarction patients. The physical examination of the patient, the electrocardiogram, and the assessment of cardiac biomarkers all play an important role in the early diagnosis of acute ischemia, along with the patient's medical history.

Main body
The present review highlights a number of different biomarkers that are released and elevated in blood during an acute myocardial infarction.

Conclusions
Analysis of cardiac biomarkers has become the first-line diagnostic tool used in the diagnosis of acute myocardial infarction. Novel markers of acute myocardial infarction, when added to routinely used markers, can provide added value not only in the earlier detection of acute myocardial infarction but also in monitoring the clinical progress of the disease, predicting its consequences, evaluating its prognosis, detecting recurrence, and managing its treatment. This leads to a lower mortality rate associated with acute myocardial infarction.
cMyC, IMA, S100, and MicroRNAs can serve as markers of early diagnosis of acute myocardial infarction, whereas myeloperoxidase, sCD40L, PAPPA, and TNF-α can be used to monitor the clinical progress of the disease. In addition, H-FABP, GDF-15, F2 isoprostanes, and ST2 can serve as predictors of AMI complications and mortality. Copeptin, ST2, and SIRT can be useful as prognostic markers of acute myocardial infarction.
Progressive collapse analysis of prestressed concrete girder bridges using improved applied element method
Authors: Abdelaziz Mohamed Magdi , Gomaa Mohamed Sayed , El-Ghazaly Hany Ahmed 
Published: December 2022

AbstractIn this paper, the Improved Applied Element Method (IAEM), which was originally developed as an effective analysis technique for large-scale bonded and unbonded prestressed structures, is utilized to carry out failure modeling of prestressed bridges under different hazard loads. A typical prestressed concrete girder bridge is analyzed under two hazardous loading scenarios. The first one is applying a detonation charge located in the middle of the central span, while the second scenario is a sudden failure of a column representing a truck or a vessel colliding with the bridge pier. For both scenarios, the collapse analyses of the bridge structure after damage are explored. In addition, the mechanism and severity of damage in the bridge pier and deck are investigated. Both material and geometric nonlinearities are considered in the analysis. Moreover, it considers contact-impact, re-contact, and inertia effects, and hence, it can track the collapse stages of the structure as well as debris movement until the complete collapse of the structure. The results show a strong capability for simulating the total performance of the bridges from early the stages of loading until the total collapse, with a clear graphic representation of the collapse phenomena.
CanRisk-Prostate: A Comprehensive, Externally Validated Risk Model for the Prediction of Future Prostate Cancer
Authors: Nyberg Tommy , Brook Mark N. , Ficorella Lorenzo , Lee Andrew , Dennis Joe , Yang Xin , Wilcox Naomi , Dadaev Tokhir , Govindasami Koveela , Lush Michael , Leslie Goska , Lophatananon Artitaya , Muir Kenneth , Bancroft Elizabeth , Easton Douglas F. , Tischkowitz Marc , Kote-Jarai Zsofia , Eeles Rosalind , Antoniou Antonis C. 
Published: December 2022

PURPOSEProstate cancer (PCa) is highly heritable. No validated PCa risk model currently exists. We therefore sought to develop a genetic risk model that can provide personalized predicted PCa risks on the basis of known moderate- to high-risk pathogenic variants, low-risk common genetic variants, and explicit cancer family history, and to externally validate the model in an independent prospective cohort.MATERIALS AND METHODSWe developed a risk model using a kin-cohort comprising individuals from 16,633 PCa families ascertained in the United Kingdom from 1993 to 2017 from the UK Genetic Prostate Cancer Study, and complex segregation analysis adjusting for ascertainment. The model was externally validated in 170,850 unaffected men (7,624 incident PCas) recruited from 2006 to 2010 to the independent UK Biobank prospective cohort study.RESULTSThe most parsimonious model included the effects of pathogenic variants in BRCA2, HOXB13, and BRCA1, and a polygenic score on the basis of 268 common low-risk variants. Residual familial risk was modeled by a hypothetical recessively inherited variant and a polygenic component whose standard deviation decreased log-linearly with age. The model predicted familial risks that were consistent with those reported in previous observational studies. In the validation cohort, the model discriminated well between unaffected men and men with incident PCas within 5 years (C-index, 0.790; 95% CI, 0.783 to 0.797) and 10 years (C-index, 0.772; 95% CI, 0.768 to 0.777). The 50% of men with highest predicted risks captured 86.3% of PCa cases within 10 years.CONCLUSIONTo our knowledge, this is the first validated risk model offering personalized PCa risks. The model will assist in counseling men concerned about their risk and can facilitate future risk-stratified population screening approaches.