bluuebunny/crossref_metadata_2025_split huggingface dataset:
A parsimonious and comprehensive screening tool for adult health behaviors
Authors: Kuo Ya‐Wen , Chang Shu‐Hung , Huang Cheng‐Hsien , Chen Mei‐Yen 
Published: April 2022

AbstractBackgroundA practical screening tool for early detection of unhealthy behaviors among adults to provide tailored care can have an important role in public health nursing.PurposeTo develop a screening tool with valid psychometric properties for adult health behaviors (AHBs).MethodsA cross‐sectional study was conducted between March 2018 and September 2019 in central‐southern Taiwan. The AHBs development included qualitative and quantitative measurement of items based on literature reviews and expert opinion and exploration and confirmatory factor analysis.ResultsA total of 765 community adult participants: 377 women and 388 men, with a mean age of 39.3 (SD = 10.6), ranging from 20 to 64 years completed the AHBs screening tool. Analysis of the 23‐items AHBs scale identified six dimensions – stress management, physical activity, health responsibility, life appreciation, healthy eating, and oral hygiene accounting for 67.3% of the variance. Total scale scores were significantly associated with a criterion variable of life satisfaction.ConclusionThe AHBs scale is a suitable screening tool that can be used to identify adults’ unhealthy behaviors early, which is useful for public health nurses to conduct a regular assessment and initiate individualized health programs.
Acting to teach communication skills to nurses
Authors: del Vecchio Alexis , Moschella Phillip Charles , Lanham Janice Garrison , Zavertnik Jean Ellen 
Published: April 2022

AbstractBackgroundInterpersonal and communication skills are core competencies for nursing students. Empathetic, patient‐centred communication improves patient outcomes and the care experience. Nursing trainees have reported a lack of preparation and confidence in communication and interpersonal skills with patients and members of the health care team. Acting‐based hands‐on training may provide a novel approach to develop communication in nursing students.ApproachThe corresponding author worked as a professional actor before pursuing a medical career. He created an acting‐based workshop, inspired by classic acting exercises taught in drama conservatories for decades, to develop core communication and interpersonal skills for health care professionals. The course creator and an instructor with no acting background each facilitated the workshops. The initiative was taught to over 200 preclinical baccalaureate freshmen and sophomore nursing students as part of their clinical skills courses at one institution.EvaluationParticipants were asked to rate their self‐efficacy for skills developed in the workshop using a 5‐point Likert scale. A 4 or 5 rating was considered agreement. Most participants agreed the workshop developed their skills of self‐awareness, observation, teamwork, flexibility, nonverbal and verbal communication, trust, mindfulness, body language awareness, active listening, and sensitivity to emotions expressed by others.ImplicationsAn acting‐based teaching intervention is efficacious in instilling core communication and interpersonal skills to preclinical nursing students based on participants' self‐efficacy ratings. This innovative way to teaching communication provides students with an experiential environment conducive to learning. Similar ratings between sessions suggest that health professions educators with no formal acting training can successfully teach this course.
Identifying Phased Mutations and Complex Rearrangements in Human Prostate Cancer Cell Lines through Linked-Read Whole-Genome Sequencing
Authors: Pham Minh-Tam , Gupta Anuj , Gupta Harshath , Vaghasia Ajay , Skaist Alyza , Garrison McKinzie A. , Coulter Jonathan B. , Haffner Michael C. , Zheng S. Lilly , Xu Jianfeng , DeStefano Shields Christina , Isaacs William B. , Wheelan Sarah J. , Nelson William G. , Yegnasubramanian Srinivasan 
Published: April 2022

Abstract

A limited number of cell lines have fueled the majority of preclinical prostate cancer research, but their genomes remain incompletely characterized. Here, we utilized whole-genome linked-read sequencing for comprehensive characterization of phased mutations and rearrangements in the most commonly used cell lines in prostate cancer research including PC3, LNCaP, DU145, CWR22Rv1, VCaP, LAPC4, MDA-PCa-2b, RWPE-1, and four derivative castrate-resistant (CR) cell lines LNCaP_Abl, LNCaP_C42b, VCaP-CR, and LAPC4-CR. Phasing of mutations allowed determination of “gene-level haplotype” to assess whether genes harbored heterozygous mutations in one or both alleles. Phased structural variant analysis allowed identification of complex rearrangement chains consistent with chromothripsis and chromoplexy. In addition, comparison of parental and derivative CR lines revealed previously known and novel genomic alterations associated with the CR phenotype.

Implications:
This study therefore comprehensively characterized phased genomic alterations in the commonly used prostate cancer cell lines, providing a useful resource for future prostate cancer research.
Inhibitor of the Nuclear Transport Protein XPO1 Enhances the Anticancer Efficacy of KRAS G12C Inhibitors in Preclinical Models of KRAS G12C–Mutant Cancers
Authors: Khan Husain Yar , Nagasaka Misako , Li Yiwei , Aboukameel Amro , Uddin Md. Hafiz , Sexton Rachel , Bannoura Sahar , Mzannar Yousef , Al-Hallak Mohammed Najeeb , Kim Steve , Beydoun Rafic , Landesman Yosef , Mamdani Hirva , Uprety Dipesh , Philip Philip A. , Mohammad Ramzi M. , Shields Anthony F. , Azmi Asfar S. 
Published: April 2022

The identification of molecules that can bind covalently to KRAS G12C and lock it in an inactive GDP-bound conformation has opened the door to targeting KRAS G12C selectively. These agents have shown promise in preclinical tumor models and clinical trials. FDA has recently granted approval to sotorasib for KRAS G12C–mutated non–small cell lung cancer (NSCLC). However, patients receiving these agents as monotherapy generally develop drug resistance over time. This necessitates the development of multi-targeted approaches that can potentially sensitize tumors to KRAS inhibitors. We generated KRAS G12C inhibitor–resistant cell lines and observed that they exhibit sensitivity toward selinexor, a selective inhibitor of nuclear export protein exportin 1 (XPO1), as a single agent. KRAS G12C inhibitors in combination with selinexor suppressed the proliferation of KRAS G12C–mutant cancer cell lines in a synergistic manner. Moreover, combined treatment of selinexor with KRAS G12C inhibitors resulted in enhanced spheroid disintegration, reduction in the number and size of colonies formed by G12C-mutant cancer cells. Mechanistically, the combination of selinexor with KRAS G12C inhibitors suppressed cell growth signaling and downregulated the expression of cell-cycle markers, KRAS and NF-κB as well as increased nuclear accumulation of tumor suppressor protein Rb. In an in vivo KRAS G12C cell-derived xenograft model, oral administration of a combination of selinexor and sotorasib was demonstrated to reduce tumor burden and enhance survival. In conclusion, we have shown that the nuclear transport protein XPO1 inhibitor can enhance the anticancer activity of KRAS G12C inhibitors in preclinical cancer models.Significance:In this study, combining nuclear transport inhibitor selinexor with KRAS G12C inhibitors has resulted in potent antitumor effects in preclinical cancer models. This can be an effective combination therapy for patients with cancer that do not respond or develop resistance to KRAS G12C inhibitor treatment.
MFN2 Prevents Neointimal Hyperplasia in Vein Grafts via Destabilizing PFK1
Authors: Tang Yuanjun , Jia Yiting , Fan Linwei , Liu Han , Zhou Yuan , Wang Miao , Liu Yuefeng , Zhu Juanjuan , Pang Wei , Zhou Jing 
Published: April 2022

Background:
Mechanical forces play crucial roles in neointimal hyperplasia after vein grafting; yet, our understanding of their influences on vascular smooth muscle cell (VSMC) activation remains rudimentary.

Methods:
A cuff mouse model was used to study vein graft hyperplasia. Fifteen percent to 1 Hz uniaxial cyclic stretch (arterial strain), 5% to 1 Hz uniaxial cyclic stretch or a static condition (venous strain) were applied to the cultured VSMCs. Metabolomics analysis, cell proliferation and migration assays, immunoblotting, co-immunoprecipitation, mutagenesis, pull-down and surface plasmon resonance assays were employed to elucidate the potential molecular mechanisms.

Results:
RNA-sequencing in vein grafts and the controls identified changes in metabolic pathways and downregulation of mitochondrial protein MFN2 (mitofusin 2) in the vein grafts. Exposure of VSMCs to 15% stretch resulted in MFN2 downregulation, mitochondrial fragmentation, metabolic shift from mitochondrial oxidative phosphorylation to glycolysis, and cell proliferation and migration, as compared with that to a static condition or 5% stretch. Metabolomics analysis indicated an increased generation of fructose 1,6-bisphosphate, an intermediate in the glycolytic pathway converted by PFK1 (phosphofructokinase 1) from fructose-6-phosphate, in cells exposed to 15% stretch. Mechanistic study revealed that MFN2 physically interacts through its C-terminus with PFK1. MFN2 knockdown or exposure of cells to 15% stretch promoted stabilization of PFK1, likely through interfering the association between PFK1 and the E3 ubiquitin ligase TRIM21 (E3 ubiquitin ligase tripartite motif [TRIM]-containing protein 21), thus, decreasing the ubiquitin-protease-dependent PFK1 degradation. In addition, study of mechanotransduction utilizing pharmaceutical inhibition indicated that the MFN2 downregulation by 15% stretch was dependent on inactivation of the SP1 (specificity protein 1) and activation of the JNK (c-Jun N-terminal kinase) and ROCK (Rho-associated protein kinase). Adenovirus-mediated MFN2 overexpression or pharmaceutical inhibition of PFK1 suppressed the 15% stretch-induced VSMC proliferation and migration and alleviated neointimal hyperplasia in vein grafts.

Conclusions:
MFN2 is a mechanoresponsive protein that interacts with PFK1 to mediate PFK1 degradation and therefore suppresses glycolysis in VSMCs.
A Preliminary Examination of Warm Season Precipitation Displacement Errors in the Upper Midwest in the HRRRE and HREF Ensembles
Authors: Kiel Benjamin M. , Gallus William A. , Franz Kristie J. , Erickson Nathan 
Published: April 2022

Abstract
Increased operational use of convection-allowing models and ensembles offers substantial improvements for some aspects of convective weather forecasting; however, errors in quantitative precipitation forecasts (QPFs) from these models, especially those related to incorrect placement of heavy rainfall systems, limit their usefulness as an input into hydrological models. To improve understanding of QPF location errors, this study quantifies the displacement errors for the centroids of both 0–18-h accumulated rainfall and rainfall in the first hour after initiation of precipitation systems in both the High-Resolution Rapid Refresh Ensemble (HRRRE) and the High-Resolution Ensemble Forecast (HREF) for 30 events in the 2018 warm season. Ensemble member QPFs are compared to quantitative precipitation estimates (QPEs) obtained from the North Central River Forecast Center (NCRFC). HRRRE is found to have less spread in centroid locations than HREF, and both HRRRE and HREF 0–18-h QPF accumulations have less spread than the 1-h QPF accumulation when the precipitation event initiates. Furthermore, QPF centroids are most often displaced to the west in HRRRE for both 0–18-h QPF accumulation and the 1-h QPF accumulation when the precipitation event initiates. The 0–18-h QPF accumulation displacement errors can be reduced when adjustments are made to the forecasted position based upon displacement errors present in the first hour of precipitation, but only when the adjustments are a function of the intercardinal quadrant in which the initial hour QPF centroid was displaced.
A Comparative Analysis of Hospital Triage Systems in the Geriatric Adult Trauma Patients: A Quality Improvement Pilot Study
Authors: Saberian Sepehr M. , Chester Daniel J. , Udobi Kahdi F. , Childs Ed W. , Danner Omar K. , Sola Richard 
Published: April 2022

Background The objective of our study is to compare the predicted hospital admission disposition based on the level of risk as determined by the modified Trauma-Specific Frailty Index (mTSFI) score with those determined by arbitrary decisions made based on the Emergency Severity Index (ESI) severity level. Methods We surveyed 100 trauma patients ages 50 and older, admitted to a level 1 trauma center between April 2019 and July 2019. We retrospectively reviewed the hospital admission disposition of each patient under the ESI, which was then compared to the mTSFI-predicted hospital admission disposition. The mTSFI scores were calculated by surveying each patient. Statistical analysis was performed to identify any statistical significance of concordance and discordance when comparing the mTSFI and ESI. Results The average age was 57.6 ± 4.2 years old in the non-geriatric group vs 76.3 ± 7.3 years old in the geriatric group. There was a male predominance in both groups (61% vs 69.5%). The mTSFI identified a higher percentage of triage discordance in the non-geriatric group (73%) compared to the geriatric cohort (53%) (95% difference CI, [39.6-40], P = .05). Discussion Non-geriatric patients have higher recorded rate of frailty than previously recognized and screening should begin at age 50, not 65. The mTSFI may be an effective tool to appropriately triage adult trauma patients at increased risk due to frailty and may reduce in-hospital complications.
Vascular Surgery Research in the Coronavirus Disease 2019 Pandemic: A Sex-Based Bibliometric Analysis
Authors: Ma Xiya , Vervoort Dominique , Babar Maryam Salma , Luc Jessica GY , Drudi Laura M 
Published: April 2022

Introduction The COVID-19 pandemic has disrupted vascular surgery services globally and its impact on researchers has illustrated disproportionate barriers for female researchers. We assessed the pandemic’s consequences on bibliometric trends in vascular surgery and vascular medicine throughout the pandemic. Methods A scoping review was performed using the PubMed/MEDLINE, Scopus, and EMBASE databases from January to December 2020 to identify articles related to COVID-19 and vascular surgery or vascular medicine. Articles only describing cardiac or neurovascular care were excluded. The scoping review was performed according to the PRISMA-ScR guidelines. Bibliometric data were extracted and analyzed. Results Four hundred and fourteen articles were identified, including 125 (30.2%) original articles, 42 (10.1%) review papers, 105 (25.4%) case reports, 27 (6.5%) editorials and commentaries, 94 (22.7%) letters and correspondences, and 21 (5.1%) conference abstracts. The 5 most common countries of study or discussion were all high-income countries. English was the predominant (n = 393, 94.9%) language. Funding was reported for 5.1% (n = 21) of articles. In the first 6 months, 17.6% (n = 30) of first authors and 10.6% (n = 18) of last authors were female, while the last 6 months saw an increase in representation to 30.6% (n = 74) and 15.6% (n = 38) for first and last author, respectively. Conclusion The pandemic caused a rapid surge in vascular publications related to COVID-19. Female authors remain underrepresented in vascular research and the share in female authorship has dropped early in the pandemic, but rose after the end of the first wave. High-income countries remain overrepresented in research productivity, alluding to important disparities in COVID-19-related literature.
Serum Albumin to Creatinine Ratio and Short-Term Clinical Outcomes in Patients With ST-Elevation Myocardial Infarction
Authors: Turkyilmaz Erdem , Ozkalayci Flora , Birdal Oğuzhan , Karagoz Ali , Tanboga Ibrahim H. , Tanalp Ali Cevat , Oduncu Vecih 
Published: April 2022

There is a lack of evidence regarding the short-term predictive value of serum albumin to creatinine ratio (sACR) in patients with ST-segment elevation myocardial infarction (STEMI). This study aims to investigate the relationship between sACR and short-term outcomes in these patients. We retrospectively enrolled 3057 patients with STEMI who underwent primary percutaneous coronary interventions (PCI) (median age was 58 years, and 74.3% were male). In-hospital mortality occurred in 114 (3.7%) patients. Contrast-induced nephropathy (CIN) was reported in 381 (12.4%) patients. During a 30-day follow-up, stent thrombosis (ST) occurred in 28 (.9%) patients and 30-day death in 147 (4.8%) patients. Multivariable logistic regression analysis reported that sACR was inversely associated with 30-day mortality (adjusted odds ratio (aOR): .51, 95% confidence interval (CI) .31–.82, P < .001). The sACR was also inversely associated with in-hospital mortality (aOR: .71, 95% CI .56–.90, P = .009), CIN (aOR: .60, 95% CI .52–.68, P < .001), congestive heart failure (CHF) (aOR: .64, 95% CI .47–.87, P = .007), and ST (aOR .61, 95% CI .41–.92, P = .001) at 30 days. Our findings suggest that sACR is inversely associated with short-term clinical outcomes in patients with STEMI after PCI.
Age Alone Is Not a Barrier to Concurrent Chemoradiotherapy for Advanced Head and Neck Cancer
Authors: Sexton Gerard P. , Walsh Paul , Moriarty Frank , O’Neill James Paul 
Published: April 2022

Background: Head and Neck Cancer (HNC) is associated with significant morbidity and mortality, especially when high stage disease is present. There exists a range of options for the management of locoregionally advanced HNC, though doubt remains as to the optimal strategy in the elderly population. Aims: To evaluate the benefits imparted by concurrent chemoradiotherapy (CCRT) to the elderly population of HNC patients in Ireland. Methods: A retrospective cohort study was conducted using 20 years of cancer registry data provided by the National Cancer Registry of Ireland. Cox multivariate regression analysis was applied to test for the benefits of CCRT in HNC. Results: Survival analysis showed an overall benefit to the use of CCRT in patients with advanced disease over 70 years, particularly when used for hypopharyngeal, oropharyngeal, and laryngeal malignancy. There was a benefit to cancer-specific but not all-cause mortality in those over 75 years, and no benefit was observed to the addition of chemotherapy in those over 80 years; only 8 patients over 80 received CCRT. There was no statistically significant difference in the benefits derived by those over 70 years relative to those under 70 years. Conclusion: CCRT confers significant survival benefits to appropriately selected elderly HNC patients and should therefore not be withheld solely on the basis of age.
Late Retropharyngeal and Parapharyngeal Abscess in Patients with a History of Anterior Cervical Discectomy and Fusion
Authors: Bivona Louis , Williamson Adrian , Emery Sanford E. , Stokes William A. 
Published: April 2022

Objective: Anterior cervical discectomy and fusion is a common procedure performed by spine surgeons with rare complications and high treatment success. Late presentation of retropharyngeal abscess in patients with a history of anterior cervical discectomy and fusion is rare but can have devastating consequences. There is a paucity of data to guide medical and surgical management of retropharyngeal abscess in these patients. Methods: We discuss 7 patients who presented to our institution with a late retropharyngeal abscess after having a history of anterior cervical discectomy and fusion. A review and description of the current literature regarding treatment and outcomes is described. Results: Seven patients presented to our institution with a retropharyngeal abscess ranging from 10 months to 7 years after undergoing anterior cervical discectomy and fusion. All patients received at least a 6-week course of appropriate intravenous antibiotics. Only one patient had their initial ACDF instrumentation removed at the time of presentation for the abscess. Four out of the 7 patients were treated with irrigation and debridement in addition to intravenous antibiotics, whereas 3 patients were treated with no surgery and intravenous antibiotics alone. All patients were asymptomatic at final follow up. Conclusions: Late retropharyngeal abscess after anterior cervical discectomy and fusion is a rare complication. Surgical management should be considered along with long term antibiotics. Removal of implants may not be necessary for infection resolution. Antibiotic treatment alone may be indicated for patients who are not septic, do not have airway compromise, or and can be considered for poor surgical candidates.
Translation, validation and exploration of the factor structure in the French version of the Posttraumatic Cognitions Inventory (PTCI)
Authors: Lebel Sarah , Dewar Michelle , Brillon Pascale 
Published: April 2022

Le Posttraumatic Cognitions Inventory (PTCI) is one of the most used instruments to assess posttraumatic cognitions. Since its release, many studies have tried to validate and translate this questionnaire, but they had difficulty to confirm its structure and then suggested alternatives. Faced with no consensus, a short version in nine statements was developed and showed good psychometric properties. To date, no French version of the PTCI has been validated, thereby preventing studies from investigating the role of posttraumatic cognitions in French speaking populations. Objectives In order to validate a French version of the PTCI, this study investigates two objectives using two French speaking samples: (1) test 10 factor structures identified in prior studies, and (2) assess the other psychometric properties of the best fitting factor structure. Method The PTCI was translated in French using a reverse translation method and administered to 202 university students and 114 aid workers. Suitability indexes of the appropriate factor structures previously identified in prior studies were examined. Internal consistency, correlations between subscales and convergent, divergent and discriminant validities in the most appropriate structure were evaluated. Results Results support that only Wells et al.'s short 9-item version of the PTCI and three factors shows excellent suitability indexes. This version also outlines an excellent internal consistency and solid convergent, divergent, and discriminant validities. Conclusions This study confirms the empirical validity, fidelity, and utility of Wells et al.'s short version of the PTCI. This is the first PTCI French validation, which is a major advantage when it comes to assess posttraumatic cognitions in French trauma victims.