bluuebunny/crossref_metadata_2025_split huggingface dataset:
Percutaneous and Arthroscopically Assisted Osteosynthesis for Displaced Calcaneal Fractures with Different Calcaneal Talar Facets: A Retrospective Study
Authors: Xia Zhang-rong , Liu Xin-yue , Zhang Lei , Li Bing-kun , Tang Xiao-gao , Chen Jun-yao , Xiong Ji-xiang , Zhou Xin 
Published: June 2022

Abstract
Background: Percutaneous and Arthroscopically Assisted Osteosynthesis (PAAO) has been reported as beneficial for calcaneal fractures because of its visibility and minimal invasiveness. But reduction effect of it is not satisfactory, for some severe displaced intra-articular fractures (DIACFs). As calcaneal fractures are frequently related to displacement of calcaneal talar facets (CTF), the purpose of this study was to explore whether the effect of PAAO is different, for calcaneus with different CTF after fractures. This can offer direction and help for clinical practice.Methods: A total of 117 patients (129 fractures) treated with PAAO were included and classified by their CTF in this study. Radiological parameters, including Bohler angle and height of calcaneus, and patient-reported outcomes, including the American Orthopedic Foot and Ankle Society (AOFAS) scores and Visual Analog Scale (VAS) were collected before surgery. The same results after surgery were analyzed and compared between different types.Results: Based on the CTF, calcaneal fractures were divided into five types ( 45 Type I, 31 Type II, 23 Type III, 18 Type IV, and 12 Type V). As for Bohler angle, Type III was smaller than Type I, II, IV and V (all P < 0.05), and Type V was larger than Type I, II, III and IV (all P < 0.05) at three time points of postoperative follow-up. The height of calcaneus of Type III was lower than that of Type I, II, IV and V (all P < 0.05), and Type V was higher than Type I, II, III and IV (all P < 0.05). There was no significant difference in AOFAS scores of five types (all P > 0.05). VAS scores were comparable between Type III (3.1) and Type I (2.2) and V (1.8)(all P < 0.05).Conclusions: The treatment effect of PAAO on DIACFs varied from different types of CTF. Therefor, PAAO can be given priority when it comes to Type V of CTF, but careful consideration should be given to whether to choose this method for Type III.
Comprehensive analysis of the transcriptome-wide m6A methylome in high-glucose and TNF-α-induced HUVECs by MeRIP sequencing
Authors: Shan Li , Wei Liang-Bing , Zhang Wei , Jiang Xiao-Xue , Fang Zhao-Hui , Gao Jia-Rong 
Published: June 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
AdipoRon engages microglia to analgesia through the AdipoR1-AMPK pathway in SNI mice
Authors: Fang Qian , Wang Yaping , Liu Xinli , Shi Yu , Chen Jiali , Zhan Hongrui , Zeng Yanyan , Wu Wen 
Published: June 2022

Abstract
Background: Microglia-associated neuroinflammation plays a crucial role in the initiation and development of neuropathic pain (NeuP). AdipoRon is an analog of adiponectin, which can activate the adiponectin receptors and exhibit anti-inflammatory property in various diseases. Adenosine monophosphate-activated protein kinase (AMPK) is the main downstream target of adiponectin receptor 1 (AdipoR1). Activation of AdipoR1 promotes the conversion of AMPK to phosphorylated AMPK (p-AMPK). The AdipoR1/AMPK pathway is involved in the regulation of inflammation. And therapies targeting this pathway are beneficial for a variety of diseases. This study aimed to investigate whether AdipoRon could alleviate NeuP by inhibiting the expression of microglia-derived tumor necrosis factor-alpha (TNF-α) through the AdipoR1/AMPK pathway.Methods: In vivo, the spared nerve injury (SNI) model was established in mice to simulate NeuP. Using the Von Frey test to detect the effect of AdipoRon on mechanical paw withdrawal threshold. Western Blot was performed to detected the effects of AdipoRon on the expression of AdipoR1, AMPK, p-AMPK and TNF-α. Immunofluorescence was performed to observed the effects of AdipoRon on spinal microglia. In vitro, using lipopolysaccharide (LPS) to induce inflammatory responses in BV2 cells. The effect of AdipoRon on cell proliferation was detected by CCK-8. Using qPCR to examined the effects of AdipoRon on the expression of TNF-α and polarization markers in LPS-treated BV2 cells. And the effect of AdipoRon on the AdipoR1/AMPK pathway was confirmed by Western Blot.Results: Intraperitoneal injection of AdipoRon alleviated mechanical pain in SNI mice. Moreover, the application of AdipoRon reduced expression of TNF-α and the number of activated microglia in the ipsilateral spinal cord. Then, AdipoRon decreased the protein level of AdipoR1 and increased the protein level of p-AMPK in the ipsilateral spinal cord. In vitro, AdipoRon inhibited BV2 cell proliferation and reversed LPS-induced TNF-α expression and polarization imbalance. Furthermore, AdipoRon abrogated the LPS-induced increase in AdipoR1 expression and decrease in p-AMPK expression in BV2 cells.Conclusions: Our results suggest that AdipoRon alleviates NeuP by inhibiting the expression of microglia-derived TNF-α through the AdipoR1/AMPK pathway.
Assessment of thermal damage in polymethyl methacrylates using quasi-static components of ultrasonic waves
Authors: Li Weibin , Jiang Chang , Xiao Jun , Xu Caibin , Deng Mingxi 
Published: June 2022

Abstract
The use of acoustic nonlinear response can be an effective method for the early-stage damage evaluation of materials. However, it is challenging to assess the thermal damage in highly attenuating materials, such as polymethyl methacrylates, by the measure of generated second harmonics with a double fundamental frequency of primary waves. Quasi-static component (QSC) generated by ultrasonic wave propagation in damaged structures, which combines the high sensitivity of acoustic nonlinearity and low attenuative effect, has a great potential for early-stage damage evaluation in highly attenuating materials. In this paper, an experimental approach for directly detecting the QSC pulse of an ultrasonic wave tone burst is proposed. While a high-frequency longitudinal wave transducer is used for exciting the primary ultrasonic wave tone burst, the co-propagating QSC pulse is captured using a low-frequency ultrasonic transducer. The phase-reversal technique is employed to enhance the signal-to-noise ratio of QSCs and to counteract the signals of primary longitudinal waves. Amplitude variations of QSCs under different thermal loadings are clearly illustrated in a repeatable manner, while those of linear ultrasonic features in the specimens are neglectable. Experimental results indicate that the measure of QSC generation of ultrasonic wave propagation can be a prospective alternative for evaluating thermal degradation of highly attenuating materials.
Accuracy and outcome of a handheld accelerometer-based navigation device in total knee arthroplasty (TKA)
Authors: Man Sheryl Lok-Chi , Chau Wai-Wang , Zheng Xieyang , Ong Michael Tim-Yun , Ho Kevin Ki-Wai 
Published: June 2022

Abstract
Background:Optimal alignment is crucial for long-term outcomes after Total knee arthroplasty (TKA). Computer-assisted surgical (CAS) techniques provide an alternative method of navigation, using a tracking device and arrays attached to bone to allow tracking of bone position in virtual space, avoiding the need for violation of intramedullary canal. The purpose of this study was to compare the accuracy of accelerometer-based KneeAlign 2 (KA2) navigation system against conventional methods for accurate positioning of femoral and tibial components in TKA.Methods:Thirty-seven and 86 consecutive cases of primary TKA were performed with either conventional or accelerometer-based navigation (ABN) system respectively. Accuracy of femoral and tibial component alignments were measured by femoral or tibial component varus/valgus angle respectively. Accuracy of tibial component alignment in the sagittal plane was measured by posterior slope of tibial component. Accuracy of conventional alignment and navigation system was assessed by measuring the difference between intraoperative goal and postoperative radiographic measurements of components.Results:No statistical difference was found in preoperative mechanical alignments of lower limb between the 2 groups. Both conventional alignment and navigation groups were able to achieve a neutral postoperative mechanical axis of the lower limb. The KA2 navigation group’s tibial component alignment in both the coronal and the sagittal planes were significantly more accurate comparing to the conventional alignment group, while there was no significant difference between the 2 groups in the femoral component alignment in the coronal plane. Mean posterior slope value within the accepted range was significantly achieved in navigation group (43%) than conventional group (16%) (p = 0.004).Conclusions:KA2 navigation was significantly more accurate than conventional alignment methods for optimal positioning of tibial component in both coronal and sagittal plane. KA2 navigation system was able to achieve such accuracy for tibial component in the sagittal plane. Both conventional alignment and KA2 navigation were able to achieve a neutral mechanical axis of lower limb, and satisfactory placement of femoral and tibial component placement in coronal plane. However, only KA2 navigation system was able to achieve such accuracy for tibial component in the sagittal plane.Trial registration: Not applicable
Towards phage therapy for acne vulgaris: Topical application in a mouse model of ‎Cutibacterium acnes-induced acne-like lesions
Authors: Rimon Amit , Rakov Chani , Lerer Vanda , Sheffer-Levi Sivan , Alkalky-Oren Sivan , Shlomov Tehila , Shasha Lihi , Lubin Ruthi , Jaber Nora , Wilensky Asaf , Coppenhagen-Glazer Shunit , Molho-Pessach Vered , Hazan Ronen 
Published: June 2022

Abstract
Acne vulgaris is a common neutrophil-driven inflammatory skin disorder in which Cutibacterium acnes (C. acnes) plays a significant role. For decades antibiotics have been widely used to treat acne vulgaris, with the inevitable increase in bacterial antibiotic resistance.Phage therapy is a promising solution to the rising problem of antibiotic-resistant bacteria, utilizing viruses that specifically lyse bacteria.Here, we explored the feasibility of phage therapy against C. acnes. Combining eight novel phages we had isolated and commonly used antibiotics, 100% of clinically isolated C. acnes strains were eradicated.Using topical phage therapy in a C. acnes-induced acne-like lesions mouse model resulted in significantly superior clinical and histological scores. Moreover, the inflammatory response decreased as reflected by reducing the chemokine CXCL2, infiltration of neutrophils, and other inflammatory cytokines, compared to the untreated group.These results demonstrate the potential of phage therapy in acne vulgaris treatment, especially when antibiotic-resistant strains are involved.
Confocal Interferometric Scattering Microscopy Reveals 3D Nanoscopic Structure and Dynamics in Live Cells
Authors: Sandoghdar Vahid , Kueppers Michelle , Albrecht David , Kashkanova Anna , Luehr Jennifer 
Published: June 2022

Abstract
Bright-field light microscopy and related techniques continue to play a key role in life sciences because they provide a facile and label-free insight into biological specimen. However, lack of three-dimensional imaging and low sensitivity to nanoscopic features hamper their application in high-end quantitative studies. Here, we remedy these shortcomings by employing confocal interferometric scattering (iSCAT) microscopy. We demonstrate the performance of this label-free technique in a selection of case studies in live cells and benchmark our findings against simultaneously acquired fluorescence images. We reveal the nanometric topography of the nuclear envelope, quantify the dynamics of the endoplasmic reticulum, detect single microtubules, and map nanoscopic diffusion of clathrin-coated pits undergoing endocytosis. Furthermore, we introduce the combination of confocal and wide-field iSCAT modalities for simultaneous imaging of cellular structures and high-speed tracking of nanoscopic entities such as single SARS-CoV2 virions. Confocal iSCAT can be readily implemented as an additional contrast mechanism in existing laser scanning microscopes.
Seasonality and weather condition’s effect on human perception of the urban-rural transitional landscape
Authors: Półrolniczak Marek , Kolendowicz Leszek 
Published: June 2022

Abstract
Landscape perception research has been around for some time now; however, research into the impact of seasonally changing landscape characteristics with the simultaneous influence of the weather on such perception are rather rare. In an attempt to fill such a lacuna, basic eye-tracking metrics were calculated according to fixation and saccades for the whole tested landscape, while its areas of interest (AOIs) were established based on clustering methods. Moreover, the gaze pattern was analysed using the Voronoi cells method. To identify significant differences in landscape perception according to various weather and seasonal conditions, nonparametric tests were applied. The significant influence of both weather and seasonality is noticed, as well as their synergistic influence on the suburban landscape visual perception. Moreover, the results indicate a rather complex influence of the analysed types of weather in warmer and cooler seasons. Regardless of the weather type, seasonal changes cause greater visual span and shorter fixations in the warmer season. The fixations and saccades are shorter in the warmer season only in two AOI’s during positive weather, but are longer in negative weather during the colder season in most AOI’s. The main reasons for the influence of weather and seasonality on the visual perception include seasonal changes in the landscape, resulting in the appearance of the landscape from more urban to natural and vice versa (phenological changes) as well as lighting changes (due to seasonality and type of weather).
Robust Formation of Optimal Single Spheroids towards Cost-Effective In-Vitro 3-Dimensional Tumor Models
Authors: Habra Kinana , Pearson Joshua R.D. , McArdle Stéphanie E. B. 
Published: June 2022

Abstract
Monolayer cell cultures, while useful for basic in vitro studies, are not physiologically relevant. Spheroids, a complex 3-dimensional (3D) structure resemble in vivo tumor growth more closely thereby allowing results obtained with spheroids relating to proliferation, cell death, differentiation, metabolism, and various anti-tumor therapies to be more predictive of in vivo outcomes. The protocol herein presents a rapid and high throughput method for the generation of single spheroids using various cancer cell lines including (U87 MG, SEBTA-027, SF188) brain cancer cells, (DU-145, TRAMP-C1) prostate cancer cells, and (BT-549, Py230) breast cancer cells in a 96-round bottom well plates. The proposed method is associated with significantly low costs (ca. £1) per plate without the need for refining or transferring and homogeneous compact spheroid morphology was evidenced as early as 1 day after following this protocol. By using confocal microscopy and the IncuCyte live imaging system, proliferating cells were traced in the rim while dead cells were found to be located inside the core region of the spheroid. H&E staining of spheroid sections was utilized to investigate the tightness of the cell packaging and Western blotting analyses revealed that these spheroids adopted a stem cell-like phenotype. This method was also used to obtain EC50 of the anti-cancer dipeptide carnosine on U87 MG 3D culture. The affordable easy-to-follow 5 step-protocol allows for robust generation of various uniform spheroids which show 3D morphology characteristics.
New Suture Probe Canaloplasty Combined with Suprachoroidal Collagen Implantation
Authors: Haus Arno , Szurman Peter , Müller Lisa , Farese Gerardo , Seuthe Anna-Maria 
Published: June 2022

Abstract

Purpose To present a modified surgery technique of suture probe canaloplasty with a specially prepared monofilament 4.0 polypropylene suture combined with suprachoroidal drainage (ScD) and collagen sheet implantation for non-penetrating glaucoma surgery.MethodsProspective study with a twelve months follow-up.In order to improve suture probe canaloplasty with a more stable und smoother probing device, a standard 4/0 polypropylene suture (Prolene™ by Ethicon) (thickness: ca. 250µm) is cut and shaped with an ophthalmic knife (MANI® Crescent Knife, Mani Inc 8-3 Kiyohara Industrial Park, Utsunomiya, Tochigi 321-3231, Japan) to create a blunt end without sharp or compressed edges. Schlemm´s canal is prepared with the standard technique of canaloplasty with suprachoroidal drainage. Then instead of using the canaloplasty microcatheter or the previously published 6.0 double-helix suture - Schlemm’s canal is probed with the blunt ending 4/0 prolene suture. In case of successful 360° probing a doubled 10/0 polypropylene tension suture is thread through the 4/0 suture, which is afterwards pulled backwards and tied at its ends. Schlemm’s canal is hereby tensioned with these 10/0 sutures. A special collagen sheet (Ologen®) is placed in suprachoroidal space, and the scleral flap is firmly sewed.Results115 eyes were included in this prospective study. In 113 cases the Schlemm´s canal could completely be probed with the suture and canaloplasty with ScD and collagen sheet implantation succeeded. In two cases the intervention was transformed to 360° suture trabeculotomy due to an intraoperative cheese-wiring. Twelve months after successful new suture probe canaloplasty with ScD and Collagen Implantation the IOP had decreased by 37.1% (from 21.6±6.0 mmHg with 3.3 different IOP lowering eye drops to 13.5±3.5mmHg with 1.0 eye drops). 16 Patients did not achieve sufficient IOP levels and underwent 360° suture trabeculotomy during the follow-up. One patient had to be treated with further glaucoma surgery to achieve a sufficient IOP level.Complications were hyphema, postoperative IOP elevation and transient hypotony. No serious or sight-threatening complications occurred.ConclusionNew suture probe canaloplasty with ScD and collagen sheet implantation yields the opportunity to conduct a cost-effective canaloplasty easier and less complicated than with the previously described method with the twisted 6/0 suture. The safety profile and IOP lowering effect is comparable. In cases where complete probing fails, there is still the opportunity to switch to suture trabeculotomy over the majorly probed part of Schlemm´s canal. The pressure lowering effect of the deep sclerectomy with ScD and suprachoroidal collagen sheet implant seems to have an additional impact on the sufficient pressure lowering procedure.
Electron-phonon coupling in amphiboles contributes to the anomalous conductivity in subduction zones
Authors: Bernardini Simone , Ventura Giancarlo Della , Schlüter Jochen Jochen , Mihailova Boriana 
Published: June 2022

Abstract
Anomalous high-conductivity layers (HCL) are typical of subduction zones, the largest recycling systems of our planet. Understanding the underlying physics of rock high conductivity has paramount implications for several planetary-scale processes, including the global water cycling, earthquake activity, and arc volcanism. Here we provide a direct proof for the activation of anisotropic electron-phonon excitations (small polarons) and delocalized H+ in riebeckite, a Fe-rich sodic amphibole typical of blue-schist metamorphic facies. This phenomenon occurs above 500 K, regardless of the oxygen fugacity, whereas the expulsion of H+ from the crystal surface occurs above 650 K only in the presence of exO2. Notably, the expected depth of activation of charge carriers, for modelled warm and cold subduction zones, fits the identified HCL providing the atomic-scale evidence for the observed anomalous electrical conductivity. This work thus gives new insights into the link between atomic-scale phenomena and Earth-scale processes interacting at convergent plate margins.
In vitro growth (IVG) of human ovarian follicles in frozen thawed ovarian cortex tissue culture supplemented with follicular fluid under hypoxic conditions.
Authors: Schallmoser Andreas , Einenkel Rebekka , Färber Cara , Sänger Nicole 
Published: June 2022

Abstract
Background: Despite its clinical success rates, transplantation after ovarian tissue cryopreservation (OTC) remains a matter of concern. Certain cancer subtypes may lead to the transfer of malignant cells when transplantation of affected ovarian tissue is conducted. IVG and subsequent isolation of vital follicles obtained from frozen thawed ovarian tissue for further in vitro maturation (IVM) would expand current fertility protection techniques while reducing the risk of retransplanting malignant cells.Methods: A total of 216 cortical biopsies from 3 patients were included in this study in 4 treatment groups. After freezing, thawing and 8 days of hypoxic tissue culture supplemented with different concentrations of human follicular fluid (HuFF) and follicle stimulating hormone (FSH), follicles were isolated enzymatically and stained with calcein in order to determine follicular viability. Numbers and size of vital follicles were assessed by fluorescence microscopy (Ti2, Nikon) and specified by computer assisted, semi-automated measurement (NIS software, Nikon). To estimate the effect of in vitro culture on apoptosis, tissue sections were stained for nicked DNA (TUNEL) prior and after tissue culture. Results: Analysing 3025 vital follicles, we observed significant differences [P <0.01] regarding follicle size when hypoxic tissue culture was supplemented with HuFF compared with the control group on day 1, individual follicles reached sizes >100µm. Conclusions: The results implicate that HuFF contains valuable factors contributing to significant IVG of follicles in human ovarian tissue and could be regarded as an additional tool in personalized fertility restoration prior to retransplantation of ovarian tissue.
Antigen-driven colonic inflammation is associated with development of dysplasia in primary sclerosing cholangitis
Authors: Jabri Bana , Shaw Dustin , Aguirre-Gamboa Raúl , Vieria Marcos , Gona Saideep , DiNardi Nicholas , Wang Anni , Dumaine Anne , Gederloos Jody , Earley Zachary , Meckel Katherine , Ciszewski Cezary , Castillo Anabella , Monroe Kelly , Torres Joana , Shah Shailja , Colombel Jean-Frederic , Itzkowitz Steven , Newberry Rodney , Cohen Russell , Rubin David , Cobey Sarah , Jonkers Iris H. , Weber Christopher , Pekow Joel , Wilson Patrick , Barreiro Luis 
Published: June 2022

Abstract
Primary sclerosing cholangitis (PSC) is an autoimmune-like disease of the bile ducts that co-occurs with inflammatory bowel disease (IBD) in almost 90% of cases. Colorectal cancer is a major complication of patients with both PSC and IBD, and these patients are at a much greater risk compared to patients with IBD without concomitant PSC. Combining flow cytometry, bulk and single cell transcriptomics, and T- and B-cell receptor repertoire analysis of right colon tissue from PSC, IBD, and healthy controls we identified a unique adaptive inflammatory transcriptional signature associated with greater risk and shorter time to dysplasia specifically in patients with PSC. This inflammatory signature is characterized by antigen-driven IL-17A+ Foxp3+ CD4 T-cell and IgG-secreting B-cell responses. These data suggest that the mechanisms of PSC and IBD dysplasia are distinct and provide molecular insights to guide prevention of colorectal cancer in PSC.