bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE EFFECT OF SOME ATTRACTIVE MEDIA ON THE NUMBER OF MARRIAGE PARTNERS, EGGS WEIGHT AND LIFETIME OF BLACK SOLDIER FLY (Hermetia illucens L.)
Authors: Lamin Syafrina , Abrar Arfan , Arwinsyah Arwinsyah , Novita Sipahutar Agnes 
Published: July 2022

The black soldier fly (BSF) Hermetia illucens L.(Diptera:Stratiomyidae) is widely used in biorecycling organic waste has gained huge populatarity in different industrial. And commercial sector becouse of its excellent potential to treat organic waste and high biomass production. The obstacle experienced during the rearing process was that a small number of eggs were obtained, therefore efforts were made to increase the black soldier fly population by providing bait in the form of attractants. Media bait technique in the form of several media with a specific and pungent aroma, so that it will lure BSF females to mate, lay eggs, extend life time. The present study deal to investigation of different number of mating pairs, eggs weight dan lifetime as function respon of different lure media.This study used a completely randomized design (CRD) with (1) cassava tape, (2) Fermented bran (3) rotten pineapple (4) rotten banana media with each treatment repeated 6 times with the variables observed were the number of mating pairs of fly (per day), egg weight, and life span of imago. each attractant medium had an effect on the number of mating females, the weight of the eggs laid and the length of life of BSF. The best medium was rotten pineapple media (number of mating pairs 55 pairs of BSF, and egg weight laid 0.007 per day) while the worst medium was fermented bran media with a long life (10.9 days)
Elective aortic arch surgery: cerebral perfusion flows and transient neurological dysfunctions
Authors: Berardi Marianna , Di Marco Luca , Leone Alessandro , Coppola Giuditta , Gliozzi Gregorio , Zanzico Federica , Brillanti Giorgia , Pacini Davide 
Published: July 2022

Aims
Selective antegrade cerebral perfusion technique is a method of cerebral protection used worldwide during aortic arch surgery. This study was designed to identify a potential correlation between perfusion flows and the development of postoperative transient neurological dysfunctions.

Methods
From January 2015 to May 2020, 175 patients underwent elective surgical replacement of the aortic arch using selective antegrade cerebral perfusion at the Cardiac Surgery Unit of Sant’Orsola Hospital in Bologna. Considering that patients who developed a permanent neurological dysfunction and those who died before a possible evaluation of neurological status were excluded, the study population included 160 patients. The perfusion flows were collected and analyzed. Univariate and multivariate analyses were performed to identify the statistical risk factors involved in the onset of transient neurological dysfunctions.

Results
The study population was divided into two groups: 138 patients (86.3%) without and 22 (13.8%) with postoperative transient neurological complications. Among the intra-operative parameters collected in the study, the univariate analysis showed that the indexed medium perfusion flow of selective antegrade cerebral perfusion was significantly lower in the transient neurological dysfunctions group (11.63 ± 2.41 ml/kg/min vs 12.62 ± 2.39 ml/kg/min, P-value = 0.03). The multivariate logistic regression analysis showed that the female gender (P = 0.004, OR = 4.816, IC = 1.636–14.174) was predictor of transient neurological dysfunctions.

Conclusion
The results of the study showed that lower perfusion flows seem to be related to a higher probability of developing transient neurological dysfunctions. However, the analysis of a wider population is required to confirm these preliminary data.
Modified Haller index is inversely correlated with true positive exercise stress echocardiographic results
Authors: Sonaglioni Andrea , Nicolosi Gian Luigi , Rigamonti Elisabetta , Lombardo Michele 
Published: July 2022

Aims
The influence of chest wall shape on exercise stress echocardiography (ESE) results has been poorly investigated. We aimed at evaluating the main predictors of true positive (TP)-ESE in a population of subjects with suspected coronary artery disease (CAD), categorized according to chest wall conformation, assessed by modified Haller index (MHI, chest transverse diameter over the distance between sternum and spine).

Methods
All consecutive patients with suspected CAD referred to our EchoLab for performing ESE between September 2011 and October 2021 were retrospectively enrolled. Preliminary assessment of both pretest probability (PTP) and MHI was performed. All patients with positive ESE underwent coronary angiography. Obstructive CAD was diagnosed by ≥70% stenosis in any epicardial coronary artery. Outcome was TP ESE result.

Results
One thousand two hundred and seventy-five consecutive patients (64.9 ± 13.0 years) entered the study. Subjects with concave-shaped chest wall (MHI > 2.5) (10.7%) and those with normal chest shape (MHI ≤ 2.5) (89.3%) were separately analyzed. PTP was similar in both groups (21.8 ± 13.2 vs. 23.5 ± 13.3%, P = 0.15). One hundred and seventy patients were diagnosed with positive ESE: 129 (75.9%) had obstructive CAD (TP), whereas the remaining 41 (24.1%) had no CAD. Only 2.3% of TP ESE was detected in subjects with MHI >2.5. PTP [odds ratio (OR) 1.03, 95% confidence interval (CI) 1.01–1.04], dyslipidemia (OR 4.37, 95% CI 2.81–6.80), dyssynergy in the left anterior descending territory (OR 8.21, 95% CI 5.07–13.3) were linearly correlated with TP ESE, whereas MHI >2.5 (OR 0.25, 95% CI 0.14–0.67) was inversely associated with outcome

Conclusions
Subjects with MHI >2.5 have low prevalence of TP ESE, regardless of PTP. Preliminary MHI assessment may reduce overestimation of PTP of CAD.
Outcome after surgical embolectomy for acute pulmonary embolism
Authors: Panholzer Bernd , Gravert Hanna , Borzikowsky Christoph , Huenges Katharina , Schoettler Jan , Schoeneich Felix , Attmann Tim , Haneya Assad , Frank Derk , Cremer Jochen , Grothusen Christina 
Published: July 2022

Aims
Patients with pulmonary embolism (PE) and contraindications for or failed thrombolysis are at the highest risk for PE-related fatal events. These patients may benefit from surgical embolectomy, but data concerning this approach are still limited.

Methods
The method used here was retrospective data analysis of 103 patients who underwent surgical embolectomy from 2002 to 2020 at our department.

Results
Mean age was 58.4 (±15.1) years. Fifty-eight (56.3%) patients had undergone recent surgery; the surgery was tumor associated in 32 (31.1%) cases. Thirty (29.1%) patients had to be resuscitated due to PE, and 13 (12.6%) patients underwent thrombolysis prior to pulmonary embolectomy. Fifteen (14.5%) patients were placed on extra corporeal membrane oxygenation (ECMO) peri-operatively. Five patients (4.9%) died intra-operatively. Neurological symptoms occurred in four patients (3.9%). Thirty-day mortality was 23.3% (n = 24). Re-thoracotomy due to bleeding was necessary in 12 (11.6%) patients. This parameter was also identified as an independent risk factor for mortality.

Conclusion
Surgical pulmonary embolectomy resulted in survival of the majority of patients with PE and contraindications for or failed thrombolysis. Given the excessive mortality when left untreated, an operative approach should become a routine part of discussions concerning alternative treatment options for these patients.
BIM Engineering Management Oriented to Curve Equation Model
Authors: Shen Fei , Ma Qiang , Salama Mohamed 
Published: July 2022

Abstract
This article uses the curve equation model to describe the initial value problem of differential equations in BIM project management. A new set of rolling curve-solving models is established for step-aligning in BIM project management. Based on the premise that the differential equation can be solved numerically, we appropriately simplify or set the required relational functions in the equation. Finally, we use mathematical software to numerically solve the differential equation and obtain the discrete function of the rolling curve. The research shows that the accuracy of the step flatness and the width of the stepped groove formed by the rolling curve of the solution in this paper is better than the original solution.
Bullous pemphigoid in India: Review of cases registered in an autoimmune bullous disease clinic
Authors: De Dipankar , Kaushik Akanksha , Handa Sanjeev , Mahajan Rahul , Chatterjee Debajyoti , Saikia Biman , Saikia Uma Nahar , Radotra Bishan Dass , Minz Ranjana W 
Published: July 2022

Background
Information on bullous pemphigoid in an Indian context is scarce.

Aim
To report clinico-demographic profile, associated comorbidities and prescription pattern of bullous pemphigoid patients in India.

Methods
This was a retrospective study, where past records of all bullous pemphigoid patients diagnosed and treated between November 2013 and October 2019 were accessed and analysed. Patients having a compatible clinical presentation with either histopathological and/or direct immunofluorescence evidence of bullous pemphigoid were included.

Results
There were 96 bullous pemphigoid patients, with a male: female ratio of 1.6:1. The mean age at diagnosis was 62.5 ± 2.2 years, with mean duration of illness 27.5 ± 4.5 months before presentation. Comorbidities were present in 80 (83%) patients, with type 2 diabetes mellitus (38.5%), hypertension (36.4%) and neurological illness (16.7%) being the commonest ones. Clinically, blisters were the predominant presentation in 81 (84.4%) patients. The majority (87.5%) of patients showed a predominant eosinophilic infiltrate on histopathology. Direct immunofluorescence revealed immunoglobulin G deposits with complement C3 in 77 (80.2%) cases. The majority of patients (77.1%) were treated with oral prednisolone, either alone (11.5%) or in combination (65.6%) with other topical and systemic agents. Topical steroids were used in 29.1%, azathioprine in 28%, dapsone in 16.7% and omalizumab in 6.2% of patients.

Limitations
The study is retrospective. Immunofluorescence on salt split skin, direct immunofluorescence serration pattern analysis, and immunoblotting were not performed. Hence, there is a possibility that a few included cases were suffering from other subepidermal autoimmune bullous diseases like epidermolysis bullosa acquisita or anti-p200 pemphigoid.

Conclusion
Bullous pemphigoid patients in this study had a younger age of onset and showed male preponderance. Comorbidities like type 2 diabetes, hypertension and neurological disorders were frequent. Cutaneous blisters were the most frequent clinical presentation. Systemic corticosteroids comprised the mainstay of therapy.
Impaired wound healing following cranial vault reconstruction in a patient with an atypical phenotype of Marfan syndrome: A case report
Authors: Recker Matthew J. , Kronenwetter Nathaniel , Reynolds Renée M. , Sadler Laurie S. , Markiewicz Michael R. 
Published: July 2022

Background:
Marfan syndrome (MFS) is an autosomal dominant disorder of the connective tissues caused by mutations in the FBN1 gene which can result in widespread systemic involvement. Loeys-Dietz syndrome (LDS) is a related autosomal dominant disorder of connective tissue with widespread systemic involvement which has phenotypic overlap with MFS. LDS is caused by heterozygous pathogenic variants in six different genes, the most common of which involve transforming growth factor beta-receptor 1 or 2. While LDS is commonly associated with craniofacial manifestations, MFS is not typically characterized by craniosynostosis.

Case Description:
We present a 7-month-old female patient with MFS and metopic craniosynostosis with an unusual clinical presentation who underwent cranial vault reconstruction with fronto-orbital advancement and anterior cranial vault remodeling. Her course was complicated by impaired wound healing after surgery, requiring return to the operating room.

Conclusion:
Phenotypic overlap between genetic disorders can confound clinical diagnosis as illustrated in this case. Genetic testing can be highly valuable in the diagnosis of clinically variable disorders. Patients with MFS who undergo cranial surgery may be at increased risk for wound healing complications.
Calvarial angiomatous meningioma developed in the diploe
Authors: Sugiyama Hiroki , Tsutsumi Satoshi , Hashizume Akane , Kuroda Kiyotaka , Sugiyama Natsuki , Ueno Hideaki , Ishii Hisato 
Published: July 2022

Background:
Angiomatous meningioma is a rare subtype of meningiomas. To the best of our knowledge, there have been no reports of intradiploic angiomatous meningioma.

Case Description:
A 53-year-old previously healthy woman was diagnosed with a calvarial lesion during a brain checkup. Cerebral magnetic resonance imaging showed an intradiploic tumor, 11 × 14 × 12 mm, in the right parietal bone. It was an enhancing, lobular tumor presenting as isointensity on T1- and hyperintensity on T2-weighted sequences, with an intense enhancement of the adjacent dura mater. Computed tomography revealed bone erosion at the tumor site, extending predominantly into the inner side, and sclerotic changes in the surrounding bone. Total resection was performed. Microscopically, the tumor tissue comprised cells with low-grade meningioma and intervening prominent vasculatures, consistent with angiomatous meningioma.

Conclusion:
Angiomatous meningioma should be considered as a differential diagnosis when an intradiploic tumor shows a lobular structure, intense enhancement of the adjacent dura mater, and sclerotic changes in the surrounding skull. These findings can support prompt tumor resection.
Spinal cord compression from cutaneous malignant peripheral nerve sheath tumor metastasis in a patient with neurofibromatosis Type 1
Authors: Calì Alessandro , Paolini Federica , Scalia Gianluca , Riolo Carmelo , Giuffrida Massimiliano , Iacopino Domenico Gerardo , Nicoletti Giovanni Federico 
Published: July 2022

Background:
Neurofibromatosis Type 1 (NF-1) and previous irradiation are two common risk factors that can result in malignant peripheral nerve sheath tumors (MPNSTs), extremely rare soft-tissue sarcomas. Here, a 63-year-old male with NF-1 presented with diffuse spinal metastases from a subcutaneous MPNST.

Case Description:
A 63-year-old male with NF-1 presented acutely with paraplegia and urinary incontinence. Both CT and MR studies of the thoracic-lumbosacral spine showed multiple metastases from a subcutaneous MPNST. In addition, the patient had a T12 vertebral body pathological fracture.

Conclusion:
Despite its aggressive behavior, some cases of MPNST can be managed with gross total resection and adjuvant radiotherapy. In addition, in the presence of multiple metastases, chemotherapy may play an additional, although questionable role.
Preschool Children Without 7-repeat DRD4 Gene More Likely to Develop Disorganized Attachment Style
Authors: Graffi Justin , Moss Ellen , Jolicoeur-Martineau Alexis , Moss Gal , Lecompte Vanessa , Pascuzzo Katherine , Babineau Vanessa , Gordon-Green Cathryn , Mileva-Seitz Viara , Minde Klaus , Sassi Roberto , Carrey Normand , Kennedy James , Gaudreau Helene , Levitan Robert , Meaney Michael , Wazana Ashley 
Published: July 2022

Background: A disorganized attachment style is characterized by fearful and disoriented behaviours when a child is separated from their caregiver. Its presence is an early predictor of the later development of psychopathology, including depression. This study seeks to determine the effects of one environmental factor - the incidence of low birth weight - and one genetic factor - the presence of the long (7-repeat) polymorphism of the dopamine D4 receptor (DRD4) gene - on the development of disorganized attachment between the child and the mother.
Methods: We sampled 251 mother-child dyads from the Maternal Adversity, Vulnerability and Neurodevelopment (MAVAN) project. We measured birth weight and DRD4 genotype, and assessed attachment style using modified separation-reunion procedure.
Results: Birth weight did not have a significant effect on disorganized attachment (b=-0.001, t(230)=0.006, p=0.910). Presence of DRD4 7-repeat polymorphism had a significant negative effect on presence of disorganized attachment style (b=-1.196, t(230)=0.411, p=0.004).
Conclusion: Children without the DRD4 7-repeat allele were more likely to display a disorganized attachment style compared to children with the DRD4 7-repeat allele. This indicates that the 7-repeat allele of the DRD4 gene may serve as a protective factor against disorganized attachment.
Fluorescence Imaging of Receptor Activator of Nuclear Factor Kappa-B Ligand-Mediated Calcium Oscillations in Osteoclasts
Authors: Stavnichuk Mariya , Sadvakassova Gulzhakhan , Komarova Svetlana 
Published: July 2022

Background: Numerous bone diseases are caused by abnormal activity of osteoclasts, cells responsible for physiological bone degradation. Understanding the mechanisms of osteoclast formation and activation is important for developing diagnostic tools and treatments for various bone diseases. Receptor activator of nuclear factor κB ligand (RANKL), a key osteoclastogenic cytokine, induces changes in intracellular Ca2+ con- centration ([Ca2+]i) that can be visualized and measured with a fluorescent Ca2+ binding dye. The objective of the study was to characterize the changes in [Ca2+]i induced by acute application of RANKL in osteoclast precursors.
Methods: We performed calcium imaging in osteoclast precursors generated from RAW 264.7 cells loaded with Fura-2 fluorescent dye using an inverted microscope, Nikon TE2000-U. Data was collected with Volocity software and analysed in Excel and MATLAB.
Results: In osteoclast precursors, RANKL induced oscillations in [Ca2+]i within 2 minutes of exposure. The main frequency of oscillations was approximately 37.7 mHz. However, no significant change in the mean level of intracellular Ca2+ was observed. Interestingly, when ATP was applied to RANKL-treated osteoclast precursors, it induced a long-lasting increase in [Ca2+]i compared to control cells.
Limitations: The limitations of our study included the small number of replicates and the short duration of fluorescence recording under each condition.
Conclusions: Short exposure of osteoclast precursors to RANKL not only induced oscillations in calcium con- centration, but also modulated cellular response to the subsequent application of ATP.
Loss-of-Function Analysis Elucidates Essential Roles of eIF4E Isoforms in Drosophila Spermatogenesis
Authors: Chen Liang 
Published: July 2022

Background: Through transcriptional and post-transcriptional regulation, eukaryotic cells can control gene expression to moderate vital cell processes and induce morphological changes. In developmental biology, translation initiation is emerging as a key player in gene expression regulation. Translation initiation begins when eukaryotic initiation factor 4E (eIF4E) binds the 5’ mRNA cap to recruit other initiation factors. Eight eIF4E isoforms are present in Drosophila melanogaster. The canonical eIF4E-1 is involved in the translation of all genes and is a common target for translational regulation mechanisms. The activity of testis-specific eIF4Es in Drosophila are largely unclear, but recent evidence on eIF4E-3 suggests that the other isoforms may also possess distinct, essential functions in spermatogenesis.
Methods: Here we provide protein localization data and loss-of-function analysis to characterize eIF4E-4, eIF4E-5, and eIF4E-7.
Results: Single KD showed few phenotypes, while eIF4E-4/eIF4E-5 double knockdown males had severe de- fects in spermatogenesis. In eIF4E-5/eIF4E-7 double knockdowns, mutations manifested in multiple stages of severity.
Conclusions: The unique expression patterns and differential mutant phenotypes observed suggest that the testis-specific isoforms contain varying levels of functional redundancy. eIF4E-4 and eIF4E-5, which share close homology, appear to have overlapping roles in regulating germ cell division during early spermato- genesis. However, during spermatid individualization they seem to assume different functions. eIF4E-7 also appears to be involved in germ cell differentiation, but most likely in a separate mechanism due to the inability of other isoforms to compensate for its knockdown.
Studying a poxvirus gene capture model through recombination and reactivation
Authors: Johnson Katherine T. , Evans Dr. David H. 
Published: July 2022

Introduction: Vaccinia poxvirus (VACV) is a double stranded DNA virus that replicates in the cytoplasm of infected cells. Some VACV genes resemble homologs of host genes and appear to have been captured from the cell; however, since poxviruses are confined to the cytoplasm, researchers are unclear as to how these viruses acquire this homology (1). If a cellular mRNA was accidentally reverse transcribed into cDNA, which could occur during retrovirus co-infection, a poxvirus might be able to incorporate this sequence into its own genome through rare non-homologous (homology-independent) recombination.
Methods: We modeled this process using two different recombination systems and substituted a DNA encoding mycophenolic acid (MPA), a selectable marker, for the hypothetical non-homologous host cDNA. We prepared DNA constructs containing this marker along with 20 base pairs homologous to the 5’ and 3’ flanking regions of the VACV-encoded NotI restriction site. A construct without this flanking homology was also prepared. The “passive” recombination system used a helper poxvirus to reactivate VACV DNA; in contrast, VACV infected BSC40 cells were transfected with the construct in the “active” recombination system.
Results: The “passive” recombination system generated 105 PFU/mL of reactivated VACV; however, no recombinants containing the selectable marker were detected. The “active recombination” method generated 106 PFU/mL of total VACV and approximately 10 PFU/mL of recombinant virus for both homology containing and non-homology containing constructs.
Discussions: We were unable to determine the recombination frequency of the “passive system” because recombinant virus was not detected. Based on virus titers determined from plaque assays, we approximated the recombination frequency of the “active system” to be ≤ 10-5. We are currently cloning and sequencing viruses resulting from non-homologous recombination to determine where the MPA marker is located. Preliminary analysis of these types of clones (data not shown in this paper) suggests that the transfected DNAs are being incorporated into a diversity of sites, some located near the boundary of the VACV genome where the right terminal inverted repeat begins. In summary, our findings suggest that the recombination frequencies in both methods are very low and better methods of selection are needed to observe these rare events. Future studies of recombinant clones are needed to gain a better understanding of this non- homologous gene capture process.