bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical significance and potential regulatory mechanism of overexpression of pituitary tumor-transforming gene transcription factor in bladder cancer
Authors: Li Sheng-Hua , Li Jian-Di , Farah Abdirahman Ahmed , Huang Zhi-Guang , Zhai Gao-Qiang , Wang Rui-Gong , Liu Jia-Lin , Wang Qin-Jie , Zhang Guan-Lan , Lei Zi-Long , Dang Yi-Wu 
Published: April 2022

Abstract
BackgroundPituitary tumor transforming gene-1 (PTTG1) transcription factor is identified carcinogenic and associated with tumor invasiveness, but its roles in bladder cancer (BLCA) remains obscure. This research is intended to analyze the aberrant expression and clinical significance of PTTG1 in BLCA, to explore the relationship between PTTG1 and tumor microenvironment characteristics, and to predict its potential transcriptional activity in BLAC tissue.MethodsWe compared the expression discrepancy of PTTG1 mRNA in BLCA and normal bladder tissue, using the BLCA transcriptomic datasets from GEO, ArrayExpress, TCGA, and GTEx. In-house immunohistochemical staining was implemented to determine the PTTG1 protein intensity. The prognostic value of PTTG1 was evaluated using the Kaplan-Meier Plotter. CRISPR screen data was utilized to estimate the effect PTTG1 interference has on BLCA cell lines. We predicted the immune cells abundance in BLCA tumor microenvironment using the microenvironment cell populations-counter and ESTIMATE algorithms. Single cell sequencing data was applied to identify the major cell types in BLCA cancer, and the dynamics of BLCA progression was revealed using pseudotime analysis. PTTG1 target genes were predicted by CistromeDB.ResultsThe elevated expression level of PTTG1 was confirmed in 1037 BLCA samples compared with 127 non-BLCA samples, with a standard mean difference value of 1.04. Higher PTTG1 expression status exhibited poorer BLCA prognosis. Moreover, the PTTG1 Chronos genetic effect scores were negative, indicating PTTG1 silence may inhibit the proliferation and survival of BLCA cells. With PTTG1 mRNA expression level increasing, higher natural killer, cytotoxic lymphocyte, and monocyte lineage cells infiltration levels were observed. A total of four candidate targets containing CHEK2, OCIAD2, UBE2L3 and ZNF367 were determined ultimately.ConclusionsPTTG1 mRNA over-expression may become a potential biomarker for BLCA prognosis. Additionally, PTTG1 may correlate with BLCA tumor microenvironment and exert transcriptional activity by targeting CHEK2, OCIAD2, UBE2L3, and ZNF367 in BLCA tissue.
Treatment of VLCAD deficient patient fibroblasts with peroxisome-proliferator activated receptor δ agonist improves cellular bioenergetics
Authors: D'Annibale Olivia , Phua Yu Leng , Land Clinton Van't , Karunanidhi Anuradha , Dorenbaum Alejandro , Mohsen Al-Walid , Vockley Jerry 
Published: April 2022

Abstract
Very long chain acyl-CoA dehydrogenase deficiency (VLCADD) is an autosomal recessive disease that prevents the body from utilizing long chain fatty acids for energy, most needed during stress and fasting. Symptoms can appear from infancy through childhood and adolescence or early adulthood, and include hypoglycemia, recurrent rhabdomyolysis, myopathy, hepatopathy, and cardiomyopathy. REN001 is a peroxisome proliferator activated receptor delta (PPARδ) agonist that modulates gene expression of fatty acid β-oxidation enzymes and oxidative phosphorylation proteins. VLCADD fibroblasts responded differently to REN001 based on genotype. All cells had statistically significant increases in ACADVL gene expression. Small increases in VLCAD protein and enzyme activity were observed and were cell line and dose dependent. Cellular bioenergetics improved in all REN001 treated fibroblasts as demonstrated by oxygen consumption rate and ATP production. VLCADD fibroblasts containing missense mutations responded better to REN001 treatment than one containing a duplication mutation in ACADVL. REN001 treated VLCADD fibroblasts results in an increase in VLCAD protein, enzyme activity, and a decrease in cellular stress. These results establish REN001 as a potential therapy for VLCADD as enhanced expression may provide therapeutic increase in total VLCAD activity but suggests the need to mutation specific treatment augmented by other treatment measures.
Carbohydrate ProLectin-M, a Galectin-3 Antagonist, Blocks SARS-CoV-2 Activity
Authors: SIGAMANI ALBEN , Chen-Walden Hana , Pahan Jayeeta , Miller Michelle C. , Mayo Kevin H , Platt David 
Published: April 2022

Abstract
The SARS-COV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) virus binds to human lectins to gain entry into cells to replicate. Blocking the virus’s entry using a complex polysaccharide component of pectin [α (1–6)- D-mannopyranose termed “ProLectin M” and Rhamnogalacturonan-II (RG-II)] that has an effect on viral replication as a therapeutic tool and a safe alternative to existing anti-viral therapies. Little is known about antagonizing galectin-3 viral-blocking activity by inhibiting viral entry into cells and subsequent viral replication, and its impact on the course of infection. Here, we investigated the effect of these non-cytotoxic polysaccharides on Vero cells infected with SARS-CoV-2 virus and demonstrated a dose-dependent reduction in viral load over a 48-hour incubation period with the virus. A pilot clinical study in five patients with laboratory-confirmed COVID-19 disease were treated with an oral formulation of α(1–6)-D-mannopyranose (ProLectin M). All patients achieved complete disease remission with zero hospitalisation or need for oxygen support. Moreover, viral load was significantly lowered within 2 days of drug administration. On the viral envelope, glycans often play a crucial role in enabling transmission of the pathogen and/or entry into its susceptible target cells. In this regard, our NMR spectroscopic studies showed on the molecular level that ProLectin M binds relatively strongly to galectin-3, supporting the idea of an antagonist effect on the lectin. Overall, our study demonstrates that blocking the viral envelope glycans can compromise entry of the virus into susceptible target cells that may translate into a positive clinical effect on the course of infection.
The role of tetradecane in the identification of host plants by the pest bugs Apolygus lucorum and Adelphocoris suturalis
Authors: Yin Haichen , Li Wenjing , Xu Min , Xu Dong , Wan Peng 
Published: April 2022

Abstract
Apolygus lucorum and Adelphocoris suturalis are considered serious pests to many cultures in China. Safe alternatives are needed to manage these mirid pests. The current study tested the olfactory responses of mirid bugs to cotton leaves, Phaseolus vulgaris pods, and its ethanol extracts. The results showed that P. vulgaris pods extracts were attractive to mirid bugs, predominantly female A. lucorum. Seven compounds from volatiles of the extract were selected to measure electrophysiological (EAG) responses of mirid bugs. Tetradecane, 2-propyl-1-pentanol, and dodecanal showed strong EAG responses in mirid bugs and, thus, used for field experiments. The results exhibited that the tetradecane showed a significantly higher attraction than other attractants with 30.33 ± 2.19 mirid bugs trapped during seven days. Lab trials showed a significant female mirid bug attraction towards tetradecane. The selected-response rates were above 60%, and it was the most attractive to females A. lucorum at the concentration of 1.5 mg/mL. Among seven tetradecane derivatives, tetradecane and tetradecanoic acid were the best attractants to A. lucorum and A. suturalis at specific concentrations. GCMS detection showed that tetradecane was present in the volatiles of 10 common hosts, and the difference of relative content was significant. The presence of tetradecane could modulate the responses of mirid bugs towards host plants suggesting that tetradecane plays a vital role in the olfactory selection of mirid bugs, while host recognition of these pests depends on many kinds of sensory cues
A changing home: a cross-sectional study on environmental degradation, resettlement and psychological distress in a Western German coal mining region
Authors: Krüger Theresa , Kraus Thomas , Kaifie Andrea 
Published: April 2022

Abstract

Background: Unwelcome changes to familiar home environments can provoke emotional and psychological distress, known as ‘solastalgia’. In Germany, the world's largest producer of brown coal, environments are being degradaded and villages resettled until today, to make way for mine developments. These environmental alterations may lead to solastalgia, though research is scarce. We therefore investigated, to which extend open-pit mining pose risks for solastalgia and psychological disorders, such as depression, generalized anxiety and somatization, on local communities.Methods: A survey was carried out in June and July 2021 in the Rhenish mining region in Western Germany. Current and recently resettled residents of two open-pit mines (Garzweiler II and Hambach) were queried about perceived environmental stressors as well as personal, socioeconomic and health impacts of open-pit mining and resettlement, including feelings of change, place attachment and activities carried out in response. The questionnaire contained modules on depression, generalized anxiety and somatization of the Patient Health Questionnaire and items from the Environmental Distress Scale, including solastalgia.Results: A total of 620 participants responded to the survey, including n = 181 resettlers, n = 114 persons from resettlement threathened villages and n = 325 persons from villages not threatened by relocation near an open-pit mine. All groups self-reported high levels of psychological distress, around twice to 7.5 times above population average. Moderate to severe somatization levels were stated by 52.7% of respondents from resettlement threathened villages, which applied for only 28% of resettlers (p < .001). Highest symptom levels of generalized anxiety (45.4%) and depression (34.3%) were also found in participants threatened by relocation. Dust was the most frequent observed environmental hazard (up to 73%), followed by noise and increased traffic.Conclusion: The degradation and loss of home environments caused by open-pit mining are associated with an increased incidence of depressive, anxious and somatoform symptoms in local communities.
Lack of Association between Nonalcoholic Fatty Liver Disease and Intracerebral Hemorrhage: A Community-based Cohort Study
Authors: Wu Jianwei , Guo Jiahuan , Wang Anxin , Zhang Yijun , Wu Shouling , Zhao Xinquan 
Published: April 2022

Abstract
Background: This study aimed to investigate whether Nonalcoholic Fatty Liver Disease (NAFLD) and its severity predict future intracerebral hemorrhage (ICH) events.Methods: We used data from the Kailuan study. Participants free of history of stroke, myocardial infarction, cancer, other liver diseases or alcohol abuse were enrolled in this study. Abdominal ultrasonography was used to diagnose NAFLD and access its severity. We stratified the participants into different groups including nonfatty liver, mild, moderate and severe NAFLD. Multivariable Cox proportional hazards regression models, adjusted for potential covariates, were applied to estimate the Hazard ratios (HRs) and 95% CI of ICH events in the 11-year follow-up.Results: A total of 77,461 participants enrolled in our study. Among them, NAFLD was diagnosed in 23,890 (30.83%) participants, including 15,581 (20.11%) mild NAFLD, 6,839 (8.83%) moderate NAFLD and 1,470 (1.90%) severe NAFLD. We documented 692 ICH events during 848,579 person years of follow-up. Patients with more severe NAFLD tended to be older, had higher levels of BMI, higher proportions of hypertension, diabetes and other known risk factors of cerebrovascular disease. However, we did not find significant associations between NAFLD, its severity and incident ICH events. Relative to nonfatty liver participants, HRs for participants with mild NAFLD, moderate and severe NAFLD were 0.98 (95% CI, 0.80 to 1.20), 1.19 (95% CI, 0.92 to 1.54) and 1.29 (95% CI, 0.81 to 2.06), respectively. Conclusions: NAFLD and its severity appear not to be significantly associated with ICH after adjustment for potential risk factors.Trial registration: The Kailuan study was registered at International Clinical Trials Registry Platform (Unique identifier: ChiCTR-TNRC-11001489, date of registration: 24/08/2011).
Urbanization and Physical Activity in the Global Prospective Urban and Rural Epidemiology Study
Authors: Boakye Kwadwo , Bovbjerg Marit , Schuna John , Adam Branscum , Varma D Ravi , Ismail Rosnah , Barbarash Olga , Dominguez Juan , Altuntas Yuksel , Anjana Ranjit , Yusuf Rita , Kelishadi Roya , Jaramillo Patricio , Iqbal Romaina , Seron Pamela , Rosengren Annika , Poirier Paul , Lakshmi PVM , Khatib Rasha , Zatońska Katarzyna , Hu Bo , Yin Lu , Wang Chuangshi , Yeates Karen , Chifamba Jephat , Alhabib Khalid F , Avezum Álvaro , Das Antonio , Lear Scott , Yusuf Salim , Hystad Perry 
Published: April 2022

Abstract
The world is rapidly urbanizing, which may influence physical activity (PA) levels - although little evidence is available for low- and middle-income countries. We evaluated associations between urbanization and total PA, as well as work-, leisure-, home-, and transport-specific PA, for 138,206 adults (35-70 years) living in 698 communities across 22 countries within the Prospective Urban and Rural Epidemiology (PURE) study. The 1-week total PA long-form International PA Questionnaire (IPAQ) was administered at baseline (2003-2015) and we used satellite-derived population density and impervious surface area to quantify levels of urbanization for 5 and 10 years prior to PA measurements. Generalized linear mixed effects models were used to examine associations between urbanization measures and PA, controlling for individual, household and community factors. Higher community baseline levels of population density (-12.4%, 95% CI: -16.0%, -8.7% per IQR) and impervious surface area (-29.2%, 95% CI: -37.5%, -19.7% per IQR), as well as 5-year population density change (-17.2%, 95% CI: -25.7%, -7.7 per IQR) was associated with lower total PA. Important differences in the associations between urbanization metrics and PA were observed between PA domains, country income levels, urban and rural status, and gender. These findings provide new information on the complex associations between urbanization and PA.
The relationship between self-assessment living standard and mental health among the elderly in rural China: the mediating role of sleep quality
Authors: Ding Hong , Zhang Beibei , Wang Xianwen , Liu Song , Zhang Min , He Xinran , Zhu Weizheng 
Published: April 2022

Abstract
Objective: Psychological problems are the main cause of anxiety, depression and happiness reduction in the elderly. Mental health imbalance not only has a direct impact on physical health, but also has an indirect impact on social support, quality of life, and brings a huge burden of disease. The purpose of this study was to explore the relationship between self- assessment living standard and mental health and the mediating role of sleep quality among the elderly in rural areas of China. Methods: Using typical field sampling method, Mengcheng County, Anhui Province was selected as the investigation site, and a total of 1223 respondents were selected. With the help of questionnaires, face-to-face interviews were used to collect data. Self- assessment living standard can be divided into five subjective dimensions. Mental health was evaluated by 12 Items General Health Questionnaire (GHQ-12). Sleep quality was measured by Pittsburgh Sleep Quality Scale (PSQI). Bootstrap test was used for data analysis.Results: The results showed that 24.7% of the elderly had a tendency to have mental health problems, which was similar to 18.5%-24.47% of the general elderly population. 59.3% of the elderly had a general self-assessment living standard (average score was 2.89 ± 0.726). The average sleep quality score was (6.97 ± 4.066), and 2.5% of the respondents reported serious sleep problems. Elderly with low self- assessment living standards were more likely to report a higher propensity for psychological problems (β=0.420, P < 0.001) and poorer sleep quality (β= 0.608, P < 0.001) than elderly with high self- assessment living standards. Mental health of the elderly may be related to sleep quality (β= 0.117, P < 0.001). In addition, the effect of self- assessment living standard on mental health was significantly mediated by sleep quality (β= 0.071, P < 0.001).Conclusions: Mental health is associated with self-assessment living standard, with this association mediated by sleep quality. A reasonable mechanism needs to be established to improve self-assessment living standard and sleep quality.
Does CAMEL affect financial performance of banks in emerging economy? From post IFRS adoption audited financial statement.
Authors: Abebe Gobena 
Published: April 2022

Abstract
To sustain the development of the economy, the performance and health of banks has to be patterned and assessed periodically. This study aimed to examine the impact of CAMEL components on banks performance in Ethiopia. The distinctive feature of this study is use of data that is from post IFRS adoption audited financial statement and it ensure the reliability and accuracy of data. From total 22 banks, 13 were taken as sample of the study based on availability and accessibility of their data for the period 2018 to 2021 which is post IFRS adoption. Ordinary least square regression with their assumption has been employed to investigate the level of impact of independent variables (Capital adequacy, Assets quality, Management Efficiency, Earnings ability and Liquidity) on the dependent variable (ROA & ROE). The study found that Capital adequacy, Asset quality and management efficiency have statistically significant effect on performance measured by ROA and ROE. Earning ability and Liquidity has no significant effect on performance measured by ROA and ROE. So it is advisable for banks to give their attention to Capital adequacy, Asset quality and management efficiency to improve their performance
The techniques, short and long term outcomes of laparoscopic transverse colectomy comparing to laparoscopic hemicolectomy in mid-transverse colon cancer.
Authors: Sun Rui , Zhang Guannan , Sun Xiyu , Niu Beizhan , Zhou Jiaolin , Cong Lin , Qiu Huizhong , Lin Guole , Wu Bin , Xiao Yi 
Published: April 2022

Abstract
Purpose: Our study was aimed to depict and summarize the technique, short and long term outcomes of laparoscopic transverse colectomy in one single clinical center and compare with laparoscopic hemicolectomy.Methods: This was a retrospective cohort study of patients with mid-transverse colon cancer in one single clinical center from February 2012 to October 2020. The enrolled patients were divided into two groups undergoing laparoscopic transverse colectomy and laparoscopic hemicolectomy, respectively. The intraoperative, postoperative complications, oncological outcomes and functional outcomes were compared between the two groups. The primary endpoint was disease free survival (DFS).Results: The study enrolled 70 patients with 40 patients undergoing laparoscopic transverse colectomy and 30 patients undergoing hemicolectomy. The intraoperative accidental hemorrhage and multiple organ resection occurred similarly in the two groups. In transverse colectomy, caudal-to-cephalic approach was likely to harvest more lymph nodes although require more operation time than cephalic-to-caudal approach (23.1(14.3) versus 13.4(5.4) lymph nodes, P=0.004; 184.3(37.1)min versus 146.3(44.4)min, P=0.012). The laparoscopic transverse colectomy was marginally associated with lower incidence of overall postoperative complications and shorter postoperative hospital stay although without statistical significance (8(20.0%) versus 12(40.0%), P=0.067; 7(5-12) VS 7(5-18), P=0.060). The 3-year DFS showed no significant difference (3-year DFS 89.7% in transverse colectomy versus 89.9% in hemicolectomy, P=0.688) between the two groups. The alternating consistency of defecation occurred significantly less after transverse colectomy (15(51.7%) VS 20(80.0%), P=0.030). Conclusions: The laparoscopic transverse colectomy is technically, oncologically and functionally feasible for mid-transverse colon cancer. Performing the caudal-to-cephalic approach might be more advantageous in lymphadenectomy.
Therapist voices on a youth mental health pilot: Gender, sexual and cultural responsiveness and therapy modality
Authors: Mathieson Fiona , Garrett Sue , Stubbe Maria , Hilder Jo , Tester Rachel , Fedchuk Dasha , Dunlop Abby , Dowell Anthony 
Published: April 2022

Abstract
There is a need for mental health services for young people. A large pilot project was conducted in Wellington New Zealand for young people aged 18-25 and included an individual CBT-informed therapy component. Therapists were asked about their views using two surveys, individual interviews and focus groups at various stages of the life of the pilot.Therapists commented on cultural responsiveness, therapy (delivery, modality and duration) and working with LGBTQIA+ youth. Some therapists felt CBT was imposed on them and preferred to use therapy-modalities they were familiar with. Many therapists liked CBT for its client-centred approach and reported using CBT-informed approaches with many of their clients to good effect. Some therapists felt pressured by their workplace to see clients for fewer sessions than they needed. Therapists wanted to see a more culturally diverse workforce, to increase their cultural competence through training and to have more easily available cultural supervision. There was some acknowledgement of the importance of training therapists to work competently with LGBTQIA+ young people. Involving therapists in co-design of services from the outset will likely benefit future service development.
Water regulates the residence time of Benzamidine in Trypsin
Authors: Ansari Narjes , Rizzi Valerio , Parrinello Michele 
Published: April 2022

Abstract
We simulate with state-of-the-art enhanced sampling techniques the binding of Benzamidine to Trypsin which is a much studied and paradigmatic ligand-protein system. We use machine learning methods and in particular Time-lagged Independent Component Analysis to determine efficient collective coordinates. These coordinates are used to perform On-the-fly Probability Enhanced Sampling simulations, which we adapt to calculate also the ligand residence time. Our results, both static and dynamic, are in good agreement with experiments. We underline the role of water in the unbinding process and find that the presence of a water molecule located at the bottom of the binding pocket allows via a network of hydrogen bonds the ligand to be released into the solution. On a finer scale, even when unbinding is allowed, another water molecule further modulates the exit time.