bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of blood total immunoglobulin E and eosinophils with radiological features of bronchiectasis
Authors: Ren Jiaqi , Chen Ai , Wang Jun , Chang Chun , Wang Juan , Sun Lina , Sun Yongchang Yongchang 
Published: November 2022

Abstract
Background: Our study aimed to investigate whether serum total IgE and blood eosinophils, were associated with radiological features of bronchiectasis in a Chinese cohort.
Methods: We retrospectively enrolled bronchiectasis patients who visited Peking University Third Hospital from Jan 1st, 2012 to Oct 7th, 2021. The clinical, laboratory and chest CT characteristics were analyzed in association with serum total IgE level and blood eosinophil count.
Results: A total of 125 bronchiectasis patients were enrolled, with 50.4% (63/125) female, and a mean age of 62.4±14.11 years. The median serum total IgE level and blood eosinophil count were 47.7 (19.8, 123.0) KU/L and 140 (90, 230) cells/µl, respectively. In patients with a higher than normal (normal range, 0-60 KU/L) total IgE (43.2%, n=54), more lobes were involved (4 (3, 5) vs 3 (2, 4), p=0.008), and mucus plugs were more common (25.9% vs 9.9%, p=0.017) on HRCT, as compared to those with a normal level of total IgE. The higher IgE group was more likely to have bilateral involvement (p=0.059), and had numerically higher Smith and Bhalla scores, but the differences were not statistically significant. In patients with an eosinophil count ≥150 cells/µl (49.6%, n=62), the number of lobes involved was greater (4 (3, 5) vs 3 (2, 4), p=0.015), and the Smith and Bhalla scores were higher (9 (5, 12) vs 6 (3, 9), p=0.009, 7 (5, 11) vs 5 (3, 9), p=0.036). The Smith score was correlated positively with the eosinophil count (r=0.207, p=0.020). Fractional exhaled nitric oxide (FeNO) was correlated with total IgE (r=0.404, p=0.001) and eosinophil count (r=0.310, p=0.014).
Conclusions: Our study demonstrated that serum total IgE and the blood eosinophil count were associated with the radiological extent and severity of bronchiectasis, necessitating further investigation on the role of T2 inflammation in structural abnormalities of this heterogeneous disease.
An Environmental Financial System for Accelerating the Development of Climate-friendly Products and Services
Authors: Pazos Jay 
Published: November 2022

Abstract
Global warming is becoming an existential problem, and solving it will require the broadest participation from society. One significant force available to society is the capacity of markets to redirect capital resources to solve problems. Here we propose an Environmental Financial System (EFS) to abate climate change that consists of eight principles, five stakeholders and three binding equations that define and make the system operational. EFS helps climate change abatement by developing a novel methodology for calculating the climate-friendliness of companies and finding an optimal portfolio of climate-friendly companies. The system frames the solution in ways that make it universally available, robust and easy to comprehend. EFS has two objectives: first, to supply funds to climate-friendly companies, and second, to communicate in a manner that incentivises actions and nudges patrons to modify their behaviour towards climate-friendly products, services, lifestyles and habits.
Development of a labelled-LFIA coupled with LAMP for the rapid and specific detection of B. melitensis targeting BMEI1661 gene
Authors: Ashmi Marcia , Kumar Bablu , . Sanjana , . Abhishek , Kumar Deepak , Singh Praveen 
Published: November 2022

Abstract
The primary cause of Brucellosis in sheep, goats, human and other animal species is B. melitensis. Despite being widely acknowledged as the gold standard method, the isolation and identification of B. melitensis cannot currently meet the criteria for early diagnostic strategies. Conventional PCR methods and immunological assays can detect B. melitensis, but their use in basic laboratories is constrained by the need for sophisticated instruments. A prompt and accurate diagnosis is essential to prevent the spread of infection to human and animals. The aim of this study was to set up a rapid and specific point of care diagnostic test for the detection of B. melitensis, which could be used routinely. Hence, a Loop mediated isothermal amplification (LAMP) coupled with lateral flow immunoassay (LFIA) was optimized for the specific detection of B. melitensis. The LAMP primers were tagged to generate product labelled with digoxigenin and biotin and the labelled LAMP amplicons were detected using LFIA with streptavidin-gold nanoparticle as a capture reagent. The assembly of gold nanoparticle at the test and control line yielded a characteristic red colour band. The sensitivity of B. melitensis LAMP-LFIA assay was 12.1fg. The assay was 100 fold more sensitive than conventional PCR and was in accordance with RT-PCR. The assay did not exhibit cross reactivity with the non-Brucella pathogens examined in this study or other Brucella species. The assay can be performed in a water bath at 65°C within 60 min and can detect upto12.1fg of genomic DNA isolated from the organism and 102 CFU/ml of B. melitensis in the spiked sample. Thus there is a great deal of potential for this assay to be utilised as a rapid field test for screening different secretions and excretions in suspected animals or their products which can tremendously reduce chances of disease transmission to both animal and human.
Integrated analysis of miRNA-mRNA regulatory network and functional verification of miR-338-3p in coronary heart disease
Authors: Qi Jie , Han Wenqi , Zhong Nier , Gou Qiling , Sun Chaofeng 
Published: November 2022

Abstract
Coronary heart disease is a cardiovascular disease with high morbidity and mortality. Although great progress has been made in treatment, the prognosis is still very poor. Therefore, this project aims to identify and screen potential diagnostic markers and therapeutic targets related to the progression of coronary heart disease. A total of 94 overlapping differentially expressed mRNAs and 73 differentially expressed miRNAs were identified by limma package from GSE20681, GSE12288, GSE49823 and GSE105449. Through a series of bioinformatics techniques and qPCR, we obtained 5 core miRNA-mRNA regulatory pairs, and selected miR-338-3p/RPS23 for functional analysis. Moreover, we found that RPS23 directly targets miR-338-3p by dual luciferase assay, western and qPCR. And the expression of miR-338-3p and RPS23 is negatively correlated. The AUC value of miR-338-3p is 0.847. Down-regulation of miR-338-3p can significantly inhibit the proliferation and migration of HUVEC. On the contrary, overexpression of miR-338-3p inhibited HUVEC cell apoptosis and promoted the proliferation and migration of HUVEC. In addition, overexpression of RPS23 can reverse the effect of miR-338-3p mimic on the proliferation activity of HUVECs. Overexpression of miR-338-3p significantly promotes the growth of HUVECs by down-regulating RPS23. In conclusion. The effect of miR-338-3p/RPS23 may be involved in the progression of coronary heart disease, and suggests that miR-338-3p may be a diagnostic biomarker and therapeutic target for coronary heart disease.
Analysis of risk factors for increased incidence of gallstone caused by hepatectomy: A retrospective case-control study
Authors: Zhou Jiangmin , Chen Lin , Zhang Zhiwei , Wu Biao 
Published: November 2022

Abstract
Background: An increased risk of gallstone has been observed in patients undergoing hepatectomy. This study attempted to analyze the risk factors of gallstone after hepatectomy.
Methods: From January 2013 to December 2016, Clinical data of 1452 eligible patients who underwent hepatectomy were consecutively reviewed. According to the imaging results including gallbladder ultrasound, CT, and MRI, all patients were divided into gallstone group and non-gallstone group. Univariate and multivariate logistic regressions were used to select indicators associated with gallstone formation among patients after hepatectomy.
Results: In the total sample of included patients, there were 341 patients with gallstone and 1147 patients without gallstone. The incidence of gallstones was (341/1452) 23.5%. The incidence of gallstones in primary liver cancer group was higher than benign liver tumor group (25.7% versus 18.9%, P=0.004). Univariate and multivariate logistic regression analysis showed that female gender, high body mass index (BMI), tumor located in S5 and severe postoperative complication were factors related to gallstone in patients with benign tumor of liver after hepatectomy. Besides, Child-Pugh B, low albumin, liver cirrhosis, and transcatheter arterial chemoembolization (TACE) after recurrence were factors related to gallstone in patients with primary liver cancer after hepatectomy.
Conclusions: Hepatectomy increased the risk of gallstone in both benign or malignant liver tumor, especially, when tumor was located in S5. TACE further increased the risk of gallstone in patients with postoperative recurrence.
Optical Multiparametric Bio-analyte Detection System: Design, Development and Noise Analysis
Authors: Basu Deeparati , Hossain Syed Minhaz , Das Jayoti 
Published: November 2022

Abstract
In this work, we have designed and developed a multiparametric optical detection system for bio-analyte measurement. Five interlinked optical parameters i.e. transmittance, reflectance, internal scattering, surface scattering and output power are measured simultaneously from transmitted and reflected spot images in MATLAB image processing environment. Also RGB channel information of images was studied to find further non-interlinked parameters for chromatic white light source. Detailed noise analysis of the system was performed to study the effect of individual component noise and finally a simple noise reduction method is presented. The system was tested with non-transparent and semi-transparent sensor samples for glucose sensing application and found to have quite sensitive performance inside a particular range of operation. The system was able to measure both real-time data and fixed time interval data for all the interlinked parameters simultaneously. Such sensitive, reliable and cost-effective instrument with multiparametric measurement feature has immense potential in optical biosensor field.
Improve Classification of Security Bug Reports using fasttext. A Machine Learning Based Approach
Authors: Alqahtani Sultan S. 
Published: November 2022

Abstract
Software developers must handle security bug reports (SBRs) before they are widely disclosed, and the system becomes vulnerable to hackers. Bug tracking systems may contain many securities-related reports which are unlabelled as SBRs. Therefore, finding unlabelled SBRs is a challenge to help security engineers identify these security issues fast and accurately. Although many methods have been proposed for classifying SBRs, challenging issues remain due to selecting an accurate and high-performance classification algorithm. This motivates us to tackle the challenges faced by the state-of-the-art SBRs classification methods by selecting a high-performance machine learning algorithm. Therefore, the main goal of this paper is to automate the process of determining which bug report can be labeled as SBR through the use of machine learning techniques. We first extracted 45,940 bug reports from publicly available datasets of five software repositories (e.g., the work of Peters et al. and Shu et al.). Second, we conducted a study on the classification of SBRs using machine learning, where we built a fasttext classifier. We then examined the accuracy of using fasttext in detecting SBRs. Our results show that fasttext can identify SBRs with an average F1 score of 0.81. Furthermore, we investigated the generalizability of identifying SBRs by applying cross-project validation, and our results show that the fasttext classifier achieves an average F1 value of 0.65. Data and results are available at https://github.com/isultane/fasttext_classifications.
Immune profiles in Bone marrow to predict induce therapy response for multiple myeloma patients
Authors: Zhaoyun Liu , Hongli Shen , Mei Han , Xianghong Zhao , Hui Liu , Kai Ding , Jia Song , Rong Fu 
Published: November 2022

Abstract
Purpose: To find bone marrow immune detection index which related to MM patients therapy.
Method: We analyzed ninety-three newly diagnosed MM patients retrospectively and their the stage of prognosis, the clinical and induced-therapy response datasets.
Result: In our study, Eight-color flow cytometry, a method commonly used to detect MM cell loads, was used to analyze seven bone marrow immune cell groups (B, NK, T, naive B, immature B, memory B, and plasmablast like cells). The percentage of B cells significantly decreased in stage III, compared with stage I patients, (P = 0.047, P = 0.038) according to the ISS and R-ISS. In the clinical characteristics, naive B cell numbers increased in the Hb(g/L)≥100 group, when compared with the Hb(g/L)< 100 group (P = 0.028). The immature B (P = 0.017), plasmablast like cell subgroups(P = 0.037) increased in MM patients, who achieved a very good partial response(VGPR), after four cycles of the new drug-based induced therapy. The ROC results indicated the combination of the seven immune subgroups had predictive values (AUC = 0.840; P < 0.0001).
Conclusion: comprehensively analyze seven bone marrow immune cell subgroups can be a useful approach for predicting the induce therapy response in MM patients.
A proposed novel genetic and epigenetic effects of Betulinic acid against breast cancer cells: in silico and in vitro study
Authors: Fadel Hewida H. , El-Wakeel Esraa , Donia Hanaa , El-Rahman Mohamed Abd , EL-Esseily Hadeer Adel , Sewedy Tarek El 
Published: November 2022

Abstract
Purpose Estrogen receptor (ER) and the Piwi-Like Protein 1 (PIWIL1) oncogene alter the expression and methylation of oncogenes and tumor suppressor genes. Potential cross-talk between ER and the methylated-DNA binding protein, Kaiso, and its downstream genes c-Myc and CDKN2A remains unexplored. Dual targeting of ER and PIWIL1 might be an excellent therapeutic approach.
Methods Initially, we used in silico molecular docking to identify potential blockers for ER and PIWIL1 among seven natural previously reported anticancer compounds. The best candidate was tested in vitro for its influence on KAISO expression by RT-PCR, promoter methylation of c-Myc and CDKN2A by methylation-specific PCR, cell cycle and apoptosis by flowcytometry, and cytotoxicity by MTT assay in the ER(+) MCF-7, and ER(−) MDA-231-MB breast cancer cell lines.
Results Betulinic acid (BA), docked ER-α and PIWIL1 perfectly at critical amino acids with the lowest binding energy. Moreover, ER and PIWIL1 docked Kaiso with root mean square deviation = 5.05 Å and 4.75 Å, respectively, at the critical amino acid Y537. Experimentally, BA caused a dose-dependent cytotoxicity in both MCF-7 and MDA-MB-231 cells (IC50= 21.3 ± 0.05 and 100 ± 0.34 µM), respectively. BA caused a significant dose-dependent G0/G1 cell cycle arrest, apoptosis induction, and upregulated Kaiso expression coincided with increased methylation of c-Myc and demethylation of CDKN2A promoter regions.
Conclusion: We are proposing a novel anticancer mechanism of action for BA via potential inhibition of Estrogen-ER-α and PIWIL1 signaling, modulation of KAISO expression and selective alterations in methylation state of c-Myc and CDKN2A key cancer-associated genes.
Socially Distanced: An Exploratory Study of the Relationships Between Delusional Ideation and Social Imagery Under the Lens of the COVID-19 Pandemic
Authors: Robertson Kennedy , Gold Ian , Veissiere Samuel , Robillard Rebecca , Solomonova Elizaveta 
Published: November 2022

Abstract
Purpose: Delusional ideation, defined as false beliefs and altered thinking, has long been attributed to anomalous/bizarre perceptual experiences and reasoning deficits. Recent studies, however, have challenged this view, suggesting instead that delusional ideation may have more of a social component to its phenomenology and underlying mechanisms. This study investigated associations between delusional ideation and factors of social imagery (felt presence, empathy, loneliness, and social anxiety) in healthy adults, using the COVID-19 pandemic as a natural social experiment rich in perceived threat and social isolation.
Methods: 2,200 participants completed the online “How are you coping? COVID-19 survey,” designed to measure the psychological, social, and financial impacts of the pandemic. Delusional ideation was assessed using the Peters Delusional Ideation (PDI) scale and felt presence experiences were assessed by the Other Experiences Questionnaire (OEQ). Other measures of social imagery included the Liebowitz Social Anxiety Scale (LSAS), the Interpersonal Reactivity Index (IRI) (empathy), and the UCLA Loneliness Scale.
Results: All aspects of social imagery were positively associated with delusional ideation. The strongest predictor of PDI score was felt presence (ß=.298, p<.001), followed by loneliness (ß=.222, p<.001), LSAS (social fear dimension) (ß=.135, p=.001), IRI (empathic concern scale) (ß=.101, p<.001), and IRI (empathizing with fictitious characters scale) (ß=.088, p<.001).
Conclusion: Results support the hypothesis that delusional ideation is sensitive to alterations in sociality, and delusions may involve social cognition for representing social agents. We propose that delusions and social imagery may share common mechanisms and increased propensity for imagining others may contribute to development of delusions.
Multi-Objective Based Route Selection Approach Using AOMDV in MANET
Authors: Saxena Madhvi , DUTTA SUBRATA , Neogy Sarmistha 
Published: November 2022

Abstract
Mobile ad hoc networks consist of mobile nodes in wireless mode. Because of ease deployment, low cost and easy communication, MANET has become one of the attractive research area in wireless communication. The challenging issues in MANET are limited energy of nodes, high mobility and dynamic topologies. These issues cause frequent link breakage due to this route discovery process reinitiated repeatedly. Many researchers gave their contribution for improving the existing protocols; still there are many scopes of improvements present. In this paper a novel routing algorithm MO-AOMDV (Multi Objective AOMDV) is proposed. In this algorithm route discovery and route selection is based on the quality of node and links, it denoted by G (V, L) where V present number of nodes and L present the link between nodes. In addition, every node has a local list where the remaining energy of node and list of neighbors is updated. It is developed and simulated in network simulator and the performance is compared with traditional AODV and AOMDV algorithm and comparing parameters are packet drop ratio, packet delivery ratio, and network lifetime.
Examination of a medial collateral ligament pie-crusting model by a finite element method
Authors: Matsuda Shogo , Hirakawa Masashi , Nagashima Yuu , Akase Hiroya , Kaku Nobuhiro , Tsumura Hiroshi 
Published: November 2022

Abstract
Background: Medial collateral ligament (MCL) pie-crusting is implemented to balance the soft tissue when performing total knee arthroplasty. Although studies have been conducted on pie-crusting, a virtual MCL model has not been created so far, and pie-crusting has not been examined using the finite element method (FEM). This study investigates and validates MCL models using FEM.
Methods: We developed an MCL model for finite element analysis where 19 elastic bodies (fibers) were bundled, and an adhesive part (Young's modulus of 175 kPa) was set in the gap and attached to both ends of the bone. An additional model 2 was created with nine 1 mm × 0.1 mm cuts in the center of the elastic body. Model 3 was developed considering nine and ten staggered cuts (19 total cuts) at two different locations, with a gap of 20 mm. Models 4 to 8 had 28, 38, 47, 76, and 95 cuts performed similarly. Subsequently, these models were analyzed. The left end of the bone was fixed, and rightward forces of 80 N and 120 N were applied to the models.
Results: The model was extended by 0.34 and 0.50 mm for every ten punctures added.
Conclusions: Our results suggest what model is better suited for the mechanical analysis of pie-crusting.
Grain bran hydrolysates in the production of fruit distillates
Authors: Krikunova Ludmila , Meleshkina Elena , Vitol Irina , Dubinina Elena , Obodeeva Olga 
Published: November 2022

Currently, there is an urgent need for domestic fermentation activators based on low-cost secondary raw materials. We aimed to study the effect of microbial enzyme preparations with different action on the hydrolysis of proteins and phytin of grain bran to obtain fermentation activators that could become an alternative to imported ones.
We studied wheat and rye brans; microbial enzyme preparations with cytolytic, proteolytic, and phytase action; multi-enzyme compositions; and grain bran hydrolysates. Firstly, we determined the kinetic characteristics of enzyme preparations. Secondly, we evaluated their effectiveness in the hydrolysis of the brans. Thirdly, we developed multi-enzyme compositions. Finally, we determined the concentration of soluble forms of phosphorus and free amino acids in the hydrolysates.
We determined optimal temperature and pH values for the enzyme preparations. The multi-enzyme compositions contributed to a high accumulation of reducing substances, water-soluble protein, and phosphorus. The concentration of free amino acids in the hydrolysates obtained under the action of the bran’s own enzymes was about 20% higher in the wheat samples, compared to the rye samples. However, when using multi-enzyme compositions in addition to the bran’s own enzymes, the concentration of free amino acids was 1.5 times higher in the rye hydrolysates, compared to the wheat hydrolysates.
The use of multi-enzyme compositions under optimal conditions can double the content of phosphorus and free amino acids available for yeast, compared to the control. Our results can be used for further research into using grain bran hydrolysates as an alternative source of nitrogen and phosphorus nutrition for yeast at the fermentation stage of fruit distillate production.
Wastewater reclamation trends in Thailand
Authors: Kanchanapiya Premrudee , Tantisattayakul Thanapol 
Published: November 2022

Abstract
Thailand constantly faces the problem of water scarcity, resulting from an imbalance between available water supply and increasing water demand for economic and community expansion, as well as climate change. To address this shortage, wastewater reclamation is being planned and implemented throughout the country, along with a 20-year, long-term integrated water resource management plan. Significant opportunities from municipal wastewater treatment plants (WWTPs) are dependent on the following factors: the establishment of a reuse water framework and a tangible target for treated wastewater set by local government authorities; widespread recognition and adaptation of wastewater reuse measures in the agriculture, industry, tourism and service sectors regarding climate change and water stress; and the implementation of joint investment water reuse projects between private and government agencies. However, wastewater reclamation faces some significant challenges, specifically: the limitations of regulation and monitoring for specific reuse purposes; a lack of public confidence in the water quality; the limited commercial development of reclaimed wastewater research; and difficulties in self-sustaining business models through adapting circular economy principles. This study aims to provide an overview of the wastewater reclamation, present research trends, currently operating WWTPs as well as opportunities and challenges to speed up water reuse activities in Thailand.