bluuebunny/crossref_metadata_2025_split huggingface dataset:
PEZO-1 and TRP-4 mechanosensors are involved in mating behavior inCaenorhabditis elegans
Authors: Brugman Katherine I. , Susoy Vladislav , Whittaker Allyson J. , Palma Wilber , Nava Stephanie , Samuel Aravinthan D.T. , Sternberg Paul W. 
Published: September 2022

ABSTRACTMale mating inCaenorhabditis elegansis a complex behavior with a strong mechanosensory component.C. eleganshas several characterized mechanotransducer proteins, but few have been shown to contribute to mating. Here, we investigated the roles of PEZO-1, a piezo channel, and TRP-4, a mechanotransducing TRPN channel, in male mating behavior. We show thatpezo-1is expressed in several male-specific neurons with known roles in mating. We show that, among other neurons,trp-4is expressed in the PCA sensory neuron, which monitors relative sliding between the male and the hermaphrodite and inhibits neurons involved in vulva detection. Mutations in both genes compromise many steps of mating, including initial response to the hermaphrodite, scanning, turning, and vulva detection. We performed pan-neuronal imaging during mating between freely-moving mutant males and hermaphrodites. Bothpezo-1andtrp-4mutants showed spurious activation of the sensory neurons involved in vulva detection. Intrp-4mutants, this spurious activation might be caused by PCA failure to inhibit vulva-detecting neurons during scanning. Indeed, we show that without functional TRP-4, PCA fails to detect the relative sliding between the male and hermaphrodite. Cell-specific TRP-4 expression restores PCA’s mechanosensory function. Our results demonstrate new roles for both PEZO-1 and TRP-4 mechanotransducers inC. elegansmating behavior.
Laboratory rearing alters activity and sleep patterns in the olive fruit fly
Authors: Terzidou Anastasia M. , Koveos Dimitrios S. , Papadopoulos Nikos T. , Carey James R. , Kouloussis Nikos A. 
Published: September 2022

AbstractOlive fruit flies,Bactrocera oleae(Diptera: Tephritidae) that are laboratory reared in artificial diet are essential for the genetic control techniques for this pest. However, the colony’s laboratory adaptation can affect their quality. We used the Locomotor Activity Monitor (LAM25, Trikinetics, MA, USA) to track the activity and sleep patterns of adult olive fruit flies reared as immatures in olives (F2-F3 generation) and in artificial diet (>300 generations). Counts of beam breaks caused by the adult fly activity were used as an estimation of its locomotor activity levels during the light and dark period. A longer than five minutes period of adults’ inactivity during the dark period was considered as one sleep episode. Activity levels and sleep parameters were found to be dependent on sex, mating status and rearing history. In virgin flies reared in olives, males were more active than females and increased their locomotor activity towards the end of the light period. Mating decreased the locomotor activity levels of males, but not of females olive-reared flies. Laboratory flies reared in artificial diet had lower locomotor activity levels during the light period and more sleep episodes of shorter duration compared to flies reared in olives. We describe the diurnal locomotor activity patterns ofB. oleaeadults reared in olive fruits and in artificial diet. We discuss how locomotor activity and sleep pattern differences may affect the laboratory flies’ ability to compete with wild males in the field.
Research on the impact of emergencies on the poultry market integration in China
Authors: Liu Tingting , Tang Shufen 
Published: September 2022

PurposeImproving the agricultural products market integration is conducive to developing provincial comparative advantage, optimization of agricultural and industrial organization and enhanced competitiveness. The relationship between the emergencies and the agricultural products market integration in the production and consumption provinces is of great significance for stabilizing market prices and improving the efficiency of agricultural resource allocation.Design/methodology/approachThe authors reviewed the literature on the market integration of agricultural products. Then, they adopted a two-way fixed effect model to investigate the impact of emergencies on the poultry market integration in the production and consumption provinces in China.FindingsHighly pathogenic avian influenza (HPAI) caused abnormal fluctuations in the poultry market price and decreased the poultry market integration. The negative impact of HPAI on poultry market integration was strengthened in the main production provinces and weakened in the main consumption provinces.Originality/valueThis is the first study that applies empirical analysis to identify the emergencies’ impact on the poultry market integration considering production and consumption characteristics. The results indicate that the impact of avian influenza is more serious in production provinces than in consumption provinces. Due to the heterogeneity of production and consumption provinces, the government implements precise compensation policies to resume production quickly after the disaster. It can be conductive to market integration and promote the development of agricultural products market.
Multidisciplinary optimization of electromagnetic linear actuators for direct-drive systems with a combination of static and dynamic performance
Authors: Chi Xuwen , Tan Cao , Li Bo , Lu Jiayu , Gu Chaofan , Fu Changzhong 
Published: September 2022

Purpose
The purpose of this paper is to solve the common problems that traditional optimization methods cannot fully improve the performance of electromagnetic linear actuators (EMLAs).

Design/methodology/approach
In this paper, a multidisciplinary optimization (MDO) method based on the non-dominated sorting genetic algorithm-II (NSGA-II) algorithm was proposed. An electromagnetic-mechanical coupled actuator analysis model of EMLAs was established, and the coupling relationship between static/dynamic performance of the actuator was analyzed. Suitable optimization variables were designed based on fuzzy grayscale theory to address the incompleteness of the actuator data and the uncertainty of the coupling relationship. A multiobjective genetic algorithm was used to obtain the optimal solution set of Pareto with the maximum electromagnetic force, electromagnetic force fluctuation rate, time constant and efficiency as the optimization objectives, the final optimization results were then obtained through a multicriteria decision-making method.

Findings
The experimental results show that the maximum electromagnetic force, electromagnetic force fluctuation rate, time constants and efficiency are improved by 18.1%, 38.5%, 8.5% and 12%, respectively. Compared with single-discipline optimization, the effectiveness of the multidiscipline optimization method was verified.

Originality/value
This paper proposes a MDO method for EMLAs that takes into account static/dynamic performance, the proposed method is also applicable to the design and analysis of various electromagnetic actuators.
Aging all over the place: a multidisciplinary framework that considers place and life trajectories of older adults within their communities
Authors: Levasseur Melanie , Naud Daniel , Presse Nancy , Delli-Colli Nathalie , Boissy Patrick , Cossette Benoît , Couturier Yves , Cadieux Genesse Julien 
Published: September 2022

PurposeThis conceptual paper aims to describe aging all over the place (AAOP), a federative framework for action, research and policy that considers older adults’ diverse experiences of place and life trajectories, along with person-centered care.Design/methodology/approachThe framework was developed through group discussions, followed by an appraisal of aging models and validation during workshops with experts, including older adults.FindingsEvery residential setting and location where older adults go should be considered a “place,” flexible and adaptable enough so that agingin placebecomes agingall over the place. Health-care professionals, policymakers and researchers are encouraged to collaborate around four axes: biopsychosocial health and empowerment; welcoming, caring, mobilized and supportive community; spatiotemporal life and care trajectories; and out-of-home care and services. When consulted, a Seniors Committee showed appreciation for flexible person-centered care, recognition of life transitions and care trajectories and meaningfulness of the name.Social implicationsPopulation aging and the pandemic call for intersectoral actions and for stakeholders beyond health care to act as community leaders. AAOP provides opportunities to connect environmental determinants of health and person-centered care.Originality/valueBuilding on the introduction of an ecological experience of aging, AAOP broadens the concept of care as well as the political and research agenda by greater integration of community and clinical actions. AAOP also endeavors to avoid patronizing older adults and to engage society in strengthening circles of benevolence surrounding older adults, regardless of their residential setting. AAOP’s applicability is evidenced by existing projects that share its approach.
Sport for confidence: a collaborative programme of physical activity, sport and exercise for people with Young Onset Dementia
Authors: Tilki Mary , Curran Charlotte , Burton Liz , Barrett Lyndsey 
Published: September 2022

Purpose
This paper aims to showcase an innovative programme of physical activity and sport to enhance the health and well-being of people with Young Onset Dementia (YOD). It is hoped that this will inspire similar collaborations between day and activity centres and exercise/sports facilities.

Design/methodology/approach
A case study of a collaborative programme between Peaceful Place, a day service for people with YOD and Sport for Confidence in Essex. It draws upon the observations and reflective accounts of those implementing the programme.

Findings
People with YOD chose and engaged in a range of physical activities and sports, resulting in increased fitness, flexibility and mobility. There were improvements in memory, cognition for some and reductions in anxiety and depression for most. The main benefits were fun, enjoyment, confidence, a sense of achievement, self-esteem, improved social interaction and wider relationships. Participants were also motivated to take more exercise, get outdoors and try new challenges and experiences.

Practical implications
Given the predicted rise in people with YOD, this paper offers insight into ways of supporting them to be active physically and socially, thus, improving health, well-being and quality of life.

Originality/value
This paper is the account of professionals deeply involved in all stages of negotiating, consulting, developing, overcoming challenges and implementing the programme.
Brexit and Strand Three of the 1998 Agreement: The Three Faces of East‐West
Authors: Whitten Lisa Claire 
Published: September 2022

AbstractUnder Strand Three of the 1998 Belfast ‘Good Friday’ Agreement, institutions were set up to promote the ‘harmonious and mutually beneficial development’ of the ‘totality of relationships’ between the peoples and governments of Ireland and the UK, including its devolved administrations and Crown Dependencies. According to the text of the 1998 Agreement this ‘east‐west’ dimension was to have two elements with corresponding institutions: an intergovernmental one reflected in the British‐Irish Intergovernmental Conference (BIIC) and an interjurisdictional one reflected in the British‐Irish Council (BIC). These Strand Three institutions were designed to provide fora for, respectively, intergovernmental cooperation on ‘non‐devolved Northern Ireland matters’ in the case of the BIIC and information exchange and cooperation ‘on matters of mutual interest within the competence of the relevant Administrations’ in the case of the BIC.Nowhere in the 1998 Agreement text is the concept of ‘east‐west’ used to refer to relations between Northern Ireland and Great Britain. Yet, in the wake of Brexit, and in the midst of controversy over the implications of the Protocol on Ireland / Northern Ireland, relations between Great Britain and Northern Ireland (GB–NI) have been newly framed as ‘east‐west’. The creation of this new discursive face of ‘east‐west’ relations marks an important, but little discussed, impact of Brexit on the political and constitutional landscape of the UK and Ireland. Against this backdrop, this article considers the impacts of Brexit, and the Protocol, on three faces of ‘east‐west’ relations—the BIIC, the BIC and, newly, GB–NI—and discusses their implications for the future of Strand 3 institutions and the ‘totality of relationships’ they represent.
A joint likelihood estimator of relatedness and allele frequencies from a small sample of individuals
Authors: Wang Jinliang 
Published: September 2022

Abstract

As a key parameter in population genetics, relatedness has found wide applications in molecular ecology, evolutionary biology, conservation, forensics and in studies of human inheritable diseases. It is defined as the probability that two individuals share an allele due to recent common ancestry. Many estimators have been developed to estimate relatedness from genotype data. However, they are invariably biased when a sample is small or contains a high proportion of close relatives, because allele frequencies required for inferring relatedness are poorly estimated in both cases under the impracticable and yet indispensable assumption of a large sample of unrelated genotypes.

In this study, I develop a likelihood method to estimate relatedness and allele frequencies jointly from a sample of multilocus genotypes. I propose an expectation maximization (EM) algorithm to update allele frequencies and the nine condensed identical by descent (IBD) coefficients () of each pair of sampled individuals iteratively till convergence. Relatedness between and inbreeding coefficients of individuals is then calculated from the estimated nine IBD coefficients. The EM algorithm is also implemented in the reduced non‐inbreeding model () to estimate three condensed IBD coefficients () and relatedness.

Using simulated and empirical data, I show that the new method is much less biased and more accurate than previous methods, providing almost unbiased relatedness and inbreeding estimates, when the sampled individuals are few or/and contain many close relatives. The EM algorithm for the likelihood estimator is fast enough to handle a sample with thousands of individuals and millions of markers, thanks to the parallelization using openMP and MPI. The method is implemented in a software package, EMIBD9, that runs on all major computer platforms.

This study shows allele frequencies and relatedness, although highly correlated and difficult to disentangle from each other when the only information available is a sample of multilocus genotypes, can be estimated jointly from genotype data of diallelic and multiallelic markers in a likelihood framework. The new method and software are especially useful for analysing small samples (such as ancient samples from museums, or samples from endangered species) and samples with a strong genetic structure.
Investigating the role of hand perspective in learning from procedural animations
Authors: Koning Bjorn B. de , Mok Katrina , Marcus Nadine , Ayres Paul 
Published: September 2022

AbstractBackgroundResearch indicates that animations presenting procedural instructions lead to better learning if the animation displays the procedural task from a first‐person perspective (over‐the‐shoulder) compared to a third‐person perspective (face‐to‐face).AimsThis study extends view‐perspective research by investigating whether the observation of human hands completing manipulative tasks in an animation are necessary or not.SampleSixty university students participated in the study.MethodParticipants studied two knot‐tying animations from a first‐person perspective showing hands, or a third‐person perspective showing hands, or a first‐person perspective without showing hands.ResultsResults showed that studying first‐person perspective animations resulted in higher performance on a knot‐tying task and recognition task (but not transfer task) than studying the third‐person perspective animations. The strongest effects were gained from the first‐person perspective animations showing hands, although comparable learning outcomes were often found with the no‐hands perspective animations. In addition, spatial ability was found to influence knot‐tying and recognition performance, while gender minimally interacted with performance in the different viewing perspective conditions.ConclusionsHand‐manipulative task are learned most optimally from animations when presented from a first‐person perspective, while it is not necessary to show the hands.
Alvocidib inhibits IRF4 expression via super‐enhancer suppression and adult T‐cell leukemia/lymphoma cell growth
Authors: Sakamoto Hikaru , Ando Koji , Imaizumi Yoshitaka , Mishima Hiroyuki , Kinoshita Akira , Kobayashi Yuji , Kitanosono Hideaki , Kato Takeharu , Sawayama Yasushi , Sato Shinya , Hata Tomoko , Nakashima Masahiro , Yoshiura Koh‐Ichiro , Miyazaki Yasushi 
Published: September 2022

AbstractAdult T‐cell leukemia/lymphoma (ATL) is an intractable hematological malignancy with extremely poor prognosis. Recent studies have revealed that super‐enhancers (SE) play important roles in controlling tumor‐specific gene expression and are potential therapeutic targets for neoplastic diseases including ATL. Cyclin‐dependent protein kinase (CDK) 9 is a component of a complex comprising transcription factors (TFs) that bind the SE region. Alvocidib is a CDK9 inhibitor that exerts antitumor activity by inhibiting RNA polymerase (Pol) II phosphorylation and suppressing SE‐mediated, tumor‐specific gene expression. The present study demonstrated that alvocidib inhibited the proliferation of ATL cell lines and tumor cells from patients with ATL. RNA sequencing (RNA‐Seq) and chromatin immunoprecipitation sequencing (ChIP‐Seq) disclosed that SE regulated IRF4 in the ATL cell lines. Previous studies showed that IRF4 suppression inhibited ATL cell proliferation. Hence, IRF4 is a putative alvocidib target in ATL therapy. The present study revealed that SE‐mediated IRF4 downregulation is a possible mechanism by which alvocidib inhibits ATL proliferation. Alvocidib also suppressed ATL in a mouse xenograft model. Hence, the present work demonstrated that alvocidib has therapeutic efficacy against ATL and partially elucidated its mode of action. It also showed that alvocidib is promising for the clinical treatment of ATL and perhaps other malignancies and neoplasms as well.
Cancerous pH‐responsive polycarboxybetaine‐coated lipid nanoparticle for smart delivery of siRNA against subcutaneous tumor model in mice
Authors: Sung Yi‐Jung , Guo Haochen , Ghasemizadeh Aria , Shen Xin , Chintrakulchai Wanphiwat , Kobayashi Motoaki , Toyoda Masahiro , Ogi Koichi , Michinishi Junya , Ohtake Tomoyuki , Matsui Makoto , Honda Yuto , Nomoto Takahiro , Takemoto Hiroyasu , Miura Yutaka , Nishiyama Nobuhiro 
Published: September 2022

AbstractLipid nanoparticles (LNPs) have been commonly used as a vehicle for nucleic acids, such as small interfering RNA (siRNA); the surface modification of LNPs is one of the determinants of their delivery efficiency especially in systemic administration. However, the applications of siRNA‐encapsulated LNPs are limited due to a lack effective systems to deliver to solid tumors. Here, we report a smart surface modification using a charge‐switchable ethylenediamine‐based polycarboxybetaine for enhancing tumor accumulation via interaction with anionic tumorous tissue constituents due to selective switching to cationic charge in response to cancerous acidic pH. Our polycarboxybetaine‐modified LNP could enhance cellular uptake in cancerous pH, resulting in facilitated endosomal escape and gene knockdown efficiency. After systemic administration, the polycarboxybetaine‐modified LNP accomplished high tumor accumulation in SKOV3‐luc and CT 26 subcutaneous tumor models. The siPLK‐1‐encapsulated LNP thereby accomplished significant tumor growth inhibition. This study demonstrates a promising potential of the pH‐responsive polycarboxybetaine as a material for modifying the surface of LNPs for efficient nucleic acid delivery.
Multimodal striatal neuromarkers in distinguishing parkinsonian variant of multiple system atrophy from idiopathic Parkinson's disease
Authors: Pang Huize , Yu Ziyang , Yu Hongmei , Chang Miao , Cao Jibin , Li Yingmei , Guo Miaoran , Liu Yu , Cao Kaiqiang , Fan Guoguang 
Published: September 2022

AbstractAimsTo develop an automatic method of classification for parkinsonian variant of multiple system atrophy (MSA‐P) and Idiopathic Parkinson's disease (IPD) in early to moderately advanced stages based on multimodal striatal alterations and identify the striatal neuromarkers for distinction.Methods77 IPD and 75 MSA‐P patients underwent 3.0 T multimodal MRI comprising susceptibility‐weighted imaging, resting‐state functional magnetic resonance imaging, T1‐weighted imaging, and diffusion tensor imaging. Iron‐radiomic features, volumes, functional and diffusion scalars of bilateral 10 striatal subregions were calculated and provided to the support vector machine for classificationResultsA combination of iron‐radiomic features, function, diffusion, and volumetric measures optimally distinguished IPD and MSA‐P in the testing dataset (accuracy 0.911 and area under the receiver operating characteristic curves [AUC] 0.927). The diagnostic performance further improved when incorporating clinical variables into the multimodal model (accuracy 0.934 and AUC 0.953). The most crucial factor for classification was the functional activity of the left dorsolateral putamen.ConclusionThe machine learning algorithm applied to multimodal striatal dysfunction depicted dorsal striatum and supervening prefrontal lobe and cerebellar dysfunction through the frontostriatal and cerebello‐striatal connections and facilitated accurate classification between IPD and MSA‐P. The dorsolateral putamen was the most valuable neuromarker for the classification.
Prognosis of hospital‐acquired pneumonia/ventilator‐associated pneumonia with Stenotrophomonas maltophilia versus Klebsiella pneumoniae in intensive care unit: A retrospective cohort study
Authors: Chen Shuping , Zou Dongdong 
Published: September 2022

AbstractIntroductionWe collected data on ventilator‐associated pneumonia (VAP) and hospital‐acquired pneumonia (HAP) induced by Stenotrophomonas maltophilia (SM) and Klebsiella pneumoniae (KP) and compared differences between two bacteria in mortality, duration of ventilator use, length of hospital stay, and risk factors for infection.ObjectivesThis study aimed to evaluate the prognosis and to find risk factors of SM‐HAP/VAP versus KP‐HAP/VAP in the intensive care unit (ICU).MethodsThis retrospective cohort study included patients admitted to the ICU between June 2019 and June 2021 and diagnosed with SM‐HAP/VAP or KP‐HAP/VAP. The primary outcome was 28‐day mortality.ResultsNinety‐two HAP/VAP patients (48 with SM‐HAP/VAP and 44 with KP‐HAP/VAP) were included. The 28‐day mortality was 16.7% (8/48 patients) in SM‐HAP/VAP and 15.9% (7/44 patients) in KP‐HAP/VAP (P = 0.922). After adjustment for potential confounders, the hazard ratios for 28‐day mortality in SM‐HAP/VAP were 1.3 (95% CI:0.5–3.7), 1.0 (95% CI:0.4–3.0), 1.4 (95% CI:0.5–4.0), and 1.1 (95% CI:0.4–3.4), respectively.ConclusionSM‐HAP/VAP and KP‐HAP/VAP patients in ICU might have a similar prognosis in mortality, the total duration of the artificial airway and ventilator use, the total length of ICU stay, and hospital stay. The risk factors of SM‐HAP/VAP versus KP‐HAP/VAP might be the artificial airway, ventilator use, gastric tube placement, acid suppressant and antibiotics (especially carbapenem).