bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zinc-finger protein 518 plays a crucial role in pericentromeric heterochromatin formation by linking satellite DNA to heterochromatin
Authors: Ohta Shinya , Ohzeki Jun-Ichirou , Sato Nobuko , Noma Ken-Ichi , Masumoto Hiroshi 
Published: September 2022

SummaryAneuploidy is caused by chromosomal missegregation and is frequently observed in cancers and hematological diseases. Pericentromeric heterochromatin formation is essential for proper chromosome segregation; however, it is unclear how heterochromatin is targeted to pericentromeres. In this study, we investigated the involvement of two homologous zinc-finger proteins (ZNF518A and ZNF518B) in heterochromatin formation at pericentromeres by determining the cellular localization of ZNF518, centromeric proteins such as CENP-A and CENP-B, and heterochromatin factors, including HP1 and histone H3K9 trimethylation. The results showed that various segments in ZNF518 interact with CENP-B, HP1, and G9a histone H3K9 methyltransferase. In conclusion, we identified a novel mechanism underlying pericentromeric heterochromatin formation mediated by ZNF518.
Time to budbreak is not enough: cold hardiness evaluation is necessary in dormancy and spring phenology studies
Authors: North Michael G. , Kovaleski Al P. 
Published: September 2022

SummaryDormancy of buds is an important phase in the life cycle of perennial plants growing in environments where unsuitable growth conditions occur seasonally. In regions where low temperature defines these unsuitable conditions, the attainment of cold hardiness is also required to survive. The end of the dormant period culminates in budbreak and flower emergence, or spring phenology, one of the most appreciated and studied phenological events. Despite this, we have a limited physiological and molecular understanding of dormancy, which has negatively affected our ability to model budbreak. Here we highlight the importance of including cold hardiness in studies that typically only characterize time to budbreak. We show how different temperature treatments may lead to increases in cold hardiness, and by doing so also (inadvertently) increase time to budbreak. Therefore, erroneous interpretations of data may occur by not phenotyping cold hardiness. Changes in cold hardiness were very likely present in previous experiments to study dormancy, especially when those included below freezing temperature treatments. Separating the effects between chilling accumulation and cold acclimation in future studies will be essential for increasing our understanding of dormancy and spring phenology in plants.
The structure, catalytic mechanism, and inhibitor identification of phosphatidylinositol remodeling MBOAT7
Authors: Wang Kun , Lee Chia-Wei , Sui Xuewu , Kim Siyoung , Wang Shuhui , Higgs Aidan B , Baublis Aaron J , Voth Gregory A , Liao Maofu , Walther Tobias C , Farese Robert V 
Published: September 2022

AbstractCells remodel glycerophospholipid acyl chains via the Lands cycle to adjust membrane properties. Membrane-bound O-acyltransferase (MBOAT) 7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA. MBOAT7 mutations cause brain developmental disorders, and reduced expression is linked to fatty liver disease. Further, increased MBOAT7 expression is linked to hepatocellular and renal cancers. The mechanistic basis of MBOAT7 catalysis and substrate selectivity are unknown. Here, we report the structure and a model for the catalytic mechanism of human MBOAT7. Arachidonyl-CoA and lyso-PI access the catalytic center through a twisted tunnel from the cytosol and lumenal sides, respectively. N-Terminal residues on the ER lumenal side determine phospholipid headgroup selectivity: swapping them between MBOATs 1, 5, and 7 converts enzyme specificity for different lyso-phospholipids. Finally, the MBOAT7 structure and virtual screening enabled identification of small-molecule inhibitors that may serve as lead compounds for pharmacologic development.
HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography
Authors: Li Shuoguo , Jia Xing , Niu Tongxin , Zhang Xiaoyun , Qi Chen , Xu Wei , Deng Hongyu , Sun Fei , Ji Gang 
Published: September 2022

ABSTRACTCryo-focused ion beam (cryo-FIB) milling technology has been developed for the fabrication of cryo-lamella of frozen native specimens for study by in situ cryo-electron tomography (cryo-ET). However, the precision of the target of interest is still one of the major bottlenecks limiting application. Here, we developed a new cryo-correlative light and electron microscopy (cryo-CLEM) system named HOPE-SIM by incorporating a 3D structured illumination fluorescence microscopy (SIM) system and an upgraded high-vacuum stage to achieve efficiently targeted cryo-FIB. With the 3D super resolution of cryo-SIM as well as our cryo-CLEM software, 3D-View, the correlation precision of targeting region of interest can reach to 150 nm enough for the subsequent cryo-lamella fabrication. We successfully utilized the HOPE-SIM system to prepare cryo-lamellae targeting mitochondria, centrosomes of HeLa cells and herpesvirus assembly compartment of infected BHK-21 cells, which suggests the high potency of the HOPE-SIM system for future in situ cryo-ET workflows.
Semantic and population analysis of the genetic targets related to COVID-19 and its association with genes and diseases
Authors: Papageorgiou Louis , Papakonstantinou Eleni , Diakou Io , Pierouli Katerina , Dragoumani Konstantina , Bacopoulou Flora , Chrousos George P , Eliopoulos Elias , Vlachakis Dimitrios 
Published: September 2022

AbstractSARS-CoV-2 is a coronavirus responsible for one of the most serious, modern worldwide pandemics, with lasting and multi-faceted effects. By late 2021, SARS-CoV-2 has infected more than 180 million people and has killed more than 3 million. The virus gains entrance to human cells through binding to ACE2 via its surface spike protein and causes a complex disease of the respiratory system, termed COVID-19. Vaccination efforts are being made to hinder the viral spread and therapeutics are currently under development. Towards this goal, scientific attention is shifting towards variants and SNPs that affect factors of the disease such as susceptibility and severity. This genomic grammar, tightly related to the dark part of our genome, can be explored through the use of modern methods such as natural language processing. We present a semantic analysis of SARS-CoV-2 related publications, which yielded a repertoire of SNPs, genes and disease ontologies. Population data from the 100Genomes Project were subsequently integrated into the pipeline. Data mining approaches of this scale have the potential to elucidate the complex interaction between COVID-19 pathogenesis and host genetic variation; the resulting knowledge can facilitate the management of high-risk groups and aid the efforts towards precision medicine.
Strategic stances and organizational performance: Are strategic performance measurement systems the missing link?
Authors: Aljuhmani Hasan Yousef , Ababneh Bashar , Emeagwali Lawrence , Elrehail Hamzah 
Published: September 2022

PurposeAlthough prior researchers have consistently established a significant relationship between different strategic stances and organizational performances across different research contexts, the mechanisms underlying this link remain unclear. This study attempts to fill this gap in the literature by testing the mediating effect of the use of strategic performance measurement systems (SPMS) on the relationship between strategic stances (prospector, defender, and reactor) and organizational performance in the public sector.Design/methodology/approachThis research is based on data collected by surveying 224 managers at public organizations in the Turkish Republic of Northern Cyprus (TRNC) and conducts an analysis using structural equation modeling (SEM).FindingsThe study findings show that prospector strategy is positively associated with organizational performance through the use of SPMS. The reactor strategy was negatively related to organizational performance through the use of SPMS. The defender strategy shows mixed results in terms of its effect on the use of SPMS and organizational performance.Research limitations/implicationsThe results obtained here provide strong evidence of the vitality of the use of SPMS for efficiency and effectiveness as a mediator between prospector strategy and organizational performance. To extend this position, future researchers could incorporate other contingent variables, such as structural autonomy, or use experimental design methods during economic austerity in the aftermath of the coronavirus disease 2019 (COVID-19) global pandemic.Originality/valueThis study represents an attempt to address public administration literature' general calls for grounded research that spells out to practitioners how different strategic stances are likely to affect the use of SPMS to achieve organizational performance levels in the public sector. The present study extends the public administration literature by examining the unexplored linkage of the use of SPMS through which strategic stances influence organizational performance in major public sector organizations.
Analyzing the strength of novelty and meaningfulness in astrotourism experiences: the mediating role of hedonism
Authors: Rodrigues Áurea , Loureiro Sandra Maria Correia 
Published: September 2022

Purpose
This study aims to explore the relationships among novelty, meaningfulness, hedonism and loyalty in the context of an astrotourism experience.

Design/methodology/approach
Data were collected using a convenience sample at an astrotourism event in Portugal. A self‐administered questionnaire was designed using established scales. In total, 296 usable responses were analyzed using partial least square (PLS)-structural equation modeling.

Findings
The result of the structural model shows that novelty and meaningfulness affect hedonism, which in turn influences loyalty. Novelty and meaningfulness contribute 62.8% to the variance in hedonism and 31.3% to the variability in loyalty.

Originality/value
This study deepens knowledge of the tourist behavior of astrotourists. Destinations and managers that develop astrotourism activities can also benefit from deeper knowledge about the emotional process of the astrotourism experience and its impact on behavioral loyalty.
The influence of higher learning institutions in developing the experience economy from the perspective of the urban community
Authors: Nor Azazi Noor Alyani , Shaed Maslina Mohammed , Samsurijan Mohamad Shaharudin , Ebekozien Andrew 
Published: September 2022

PurposeThe development of higher learning institutions (HLIs) is considered a strategy to trigger urban space development – and it is the economy in most developing countries. HLIs can develop and maintain pace with the experience economy in the current urban economy, particularly in the services sector. This paper seeks to evaluate the influence of HLIs on elements of the experience economy in the urban services sector in Bandar Baru Bangi (BBB), a knowledge-based city.Design/methodology/approachThe research adopted a purposive sampling technique and engaged 382 urban community respondents in BBB, Malaysia. The study used four elements (education, gastronomy, health, and the retail sectors) to assess the experience economy performance.FindingsThe results show that the local community is the “active users” of the services, and the active users have enjoyed the existence of the experience economy. Findings reveal a preference for education and health over gastronomy and retail sectors. Of these four sectors, the education sector experience had the most prominent effect, thereby showing that the higher learning institutions around this city served a major role in the sector development of urban services.Research limitations/implicationsThe research used a purposive sampling method and engaged 382 respondents in BBB, Malaysia. The restriction of the study area to BBB is a limitation component. Future studies should explore a large-scale investigation to evaluate better and validate the results.Practical implicationsThe research has shown that the city's higher education institutions have affected the development of the experience economy in the four sectors.Originality/valueThe study shows that the framework of the experience economy and the establishment of HLIs can stimulate the experience economy within the urban services sector.
Modeling entropy generation of magnetohydrodynamics flow of nanofluid in a porous medium: a review
Authors: Zahor Feda Abdalla , Jain Reema , Ali Ahmada Omar , Masanja Verdiana Grace 
Published: September 2022

PurposeThe purpose of this paper is to review previous research studies on mathematical models for entropy generation in the magnetohydrodynamics (MHD) flow of nanofluids. In addition, the influence of various parameters on the velocity profiles, temperature profiles and entropy generation was studied. Furthermore, the numerical methods used to solve the model equations were summarized. The underlying purpose was to understand the research gap and develop a research agenda.Design/methodology/approachThis paper reviews 141 journal articles published between 2010 and 2022 on topics related to mathematical models used to assess the impacts of various parameters on the entropy generation, heat transfer and velocity of the MHD flow of nanofluids.FindingsThis review clarifies the application of entropy generation mathematical models, identifies areas for future research and provides necessary information for future research in the development of efficient thermodynamic systems. It is hoped that this review paper can provide a basis for further research on the irreversibility of nanofluids flowing through different channels in the development of efficient thermodynamic systems.Originality/valueEntropy generation analysis and minimization constitute effective approaches for improving the performance of thermodynamic systems. A comprehensive review of the effects of various parameters on entropy generation was performed in this study.
The impact of entrepreneurial passion on entrepreneurial success and psychological well-being: a person-centered investigation
Authors: Chen Chunxiao , Zhang Jian , Tian Huirong , Bu Xing 
Published: September 2022

PurposeEntrepreneurial passion has important implications for entrepreneurial success and psychological well-being. But their connections are complicated by the fact that three entrepreneurial passions (passion for inventing, passion for founding and passion for developing) can be combined differently according to their level and shape difference. A variable-centered approach cannot explain their relationship very well, by only focusing on the level difference and ignoring the different combination of entrepreneurial passion in subpopulations. The purpose of this study is to explore the function of entrepreneurial passion on entrepreneurial success and psychological well-being from a person-cantered approach.Design/methodology/approachIn this study, the authors conducted latent profile analyses to identify different configurations of different entrepreneurial passions (passion for inventing, passion for founding, passion for developing) on two samples of Chinese students and entrepreneurs. Then in the sample of Chinese entrepreneurs, the authors utilized the DCON command in Mplus to provide comparisons among the profiles on entrepreneurial success and psychological well-being.FindingsBased on identity theory, the authors found four entrepreneurial passion profiles across two samples—fully passionate, action-driven, interest-driven and dispassionate. Furthermore, the authors found that fully passionate entrepreneurs showed the highest level of entrepreneurial success, followed by action-driven, and then by interest-driven and dispassionate showed the lowest level. Action-driven entrepreneurs experienced the highest level of psychological well-being, followed by fully passionate entrepreneurs, then by interest-driven and dispassionate.Originality/valueThe results provide new insights into the nature and influence of entrepreneurial passion on entrepreneurial success and psychological well-being from a person-centered perspective.
A bibliometric analysis of the elaboration likelihood model (ELM)
Authors: Srivastava Mukta , Saini Gordhan K. 
Published: September 2022

Purpose
The purpose of this study is to unpack the knowledge structure of the elaboration likelihood model (ELM), examine its applications in various domains and propose future research directions using bibliometric analysis.

Design/methodology/approach
The study involves the bibliometric analysis of 279 articles retrieved from Scopus using VOSviewer and Biblioshiny. The authors used trend topic analysis, bibliographic coupling, cluster analysis and content analysis as the analytical methods.

Findings
The study revealed the most influential authors, journals, articles and countries in the domain. The keyword analyses showed that initially, the model was used in the field of “persuasion” and “persuasive communication”; however, in the past five years, it has been widely adopted in different areas such as “crowdsourcing,” “decision support systems” and “information systems.” Bibliographic coupling combined with content analysis resulted in seven clusters: ELM in marketing communications, ELM in information system research, ELM in the service context, ELM in advertising research, ELM and electronic word-of-mouth, ELM and trust in the online context and ELM in other contexts.

Originality/value
The study provides a bibliometric analysis of the ELM, offers useful future research directions to the scholars of different domains and highlights implications for managers.
The ‘Service Transformation Outline’ toolkit to assist in the design, comparison and replication of integrated care programmes
Authors: Rossi Mori Angelo 
Published: September 2022

PurposeThe author is introducing a toolkit that can assist stakeholders to design, compare and replicate integrated care programmes, by making explicit their view on the transformations of care and cure services, according to a structured template. The purpose of this paper is to address this issue.Design/methodology/approachThe toolkit is made of two elements: a classification and a template. The author adopted a step-wise approach of semantic modelling to work out three layers of a classification in the domain of integration needs related to care and cure services. The third layer consists of 23 non-overlapping classes that fully cover that semantic domain. The classes are used to build a template to elicit the stakeholder's standpoint about the transformations involved in the deployment of a programme. The result is the «Outline» of the programme.FindingsSo far, in eight years the author applied the toolkit to 100+ programmes either to design, simulate or evaluate them, either to compare them to similar ones in the same or in different jurisdictions, and we refined the description of the classes according to that experience.Research limitations/implicationsThe objective of the toolkit is not to provide solutions, but to stimulate reflections on the transformations involved in a programme and their practical consequences in a precise context. In fact, the Outlines cannot be generalized: they are conceived to reflect the perspective of the stakeholders and thus are intrinsically subjective; in addition, they must be contingent, as they must depend on the local context in the particular timeframe. In case of similar initiatives in other localities, the Outlines must be filled in again by the local stakeholders; however, a subsequent comparison could help to explore similarities and motivated differences.Practical implicationsIdeally the stakeholders should use the Outline of the actual transformations in a programme as a reference to mediate between the principles and the methodologies provided by the «Models» and «Conceptual Frameworks» in the literature (e.g. Chronic Care Model, Rainbow Model, Development Model of Integrated Care Patient-Centered Medical Home and Maturity Models) and the deeper studies using the specific tools developed in their disciplines (e.g. on information modelling, process modelling, cost–benefit analysis and health technology assessment). The toolkit could have its role also in a multi-annual roadmap made of a sequence of programmes to cope with the urgent challenges on ageing, social changes and technological evolution, in synergy with regulations, budget, context maturity, critical success factors and local priorities.Originality/valueThe author argues that the approach of the structured Outline is unique, as the scoring mechanism to assess the relevance of the transformation within each class on the overall change brought by the whole programme into the health systems.
Innovativeness and export competitiveness in the East African Community
Authors: Olyanga Anthony Moni , Shinyekwa Isaac M.B. , Ngoma Muhammed , Nkote Isaac Nabeta , Esemu Timothy , Kamya Moses 
Published: September 2022

PurposeThe purpose of this paper is to examine the influence of innovation indicators: Internet usage, patent rights, innovation in exporting countries and innovation in the importing country on the export competitiveness of firms in the East African Community (EAC).Design/methodology/approachThe study adopted the structural gravity model and the Poisson Pseudo Maximum Likelihood a nonlinear estimation method that was applied in STATA on balanced panel data from 2007 to 2018. Data were obtained from World Bank International Trade Center and World Bank development indicators.FindingsResults show that innovation in the importing country, innovation in the exporting country and patent rights of exports are positive and significant predictors of export competitiveness in developing countries. While Internet usage is an insignificant predictor in the EAC.Research limitations/implicationsThere is a need to examine the complicated nature of the EAC economy to further this study's findings.Practical implicationsExporting countries need to take deeper reforms as regards structural transformation to enable firms to integrate into the Global Value Chains (GVCs) to enable them to increase their productivity by reviewing the existing policies to match the changes in the market.Originality/valueThis study explains the complex dynamic interactions of technological innovation indicators in the EAC using quantitative data and that this interaction has an effect on the export competitiveness in import-oriented countries with less harmonization in their trade policies.