bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of combined oral contraceptive pills on angiogenesis in endometriotic lesions
Authors: Antonis Siampalis , Efthymia Papakonstantinou , Maria Keramida , Eleftherios Panteris , Sotiris Kalogeropoulos , Neoklis Georgopoulos , Fuminori Taniguchi , George Adonakis , Tasuku Harada , Apostolos Kaponis 
Published: February 2025

Abstract

Purpose
Neoangiogenesis is necessary for adhesion and invasion of endometriotic lesions. We hypothesize that by blocking angiogenetic pathways we can suppress endometriosis. Oral contraceptive pills (OCs) are routinely used in endometriosis to suppress symptoms of the disease. In the current study, we attempt to evaluate the effects of OCs on various angiogenetic factors in women with endometriosis.

Methods
Sixty women with endometriosis were randomly divided into two groups. Group A consisted of 30 women who received OCs in a cyclical manner for 3 months before surgery and group B of 30 women who did not. Biopsy specimens of ovarian endometrioma were collected. We used qRT-PCR to study the mRNA expression levels of VEGF, TF, PAR-2, SP1, and FGF1.

Results
The levels of mRNA of all angiogenic factors were found to be elevated in women who received OCs compared with women who did not. This difference was statistically significant for VEGF, TF, FGF1, SP1 (p < 0.001), and PAR-2 (p = 0.046).

Conclusion
OC administration does not inhibit neoangiogenesis in endometriotic lesions; on the contrary, angiogenetic pathways might be upregulated.
Machine learning approach for predicting the compressive strength of biomedical waste ash in concrete: a sustainability approach
Authors: Kumar Rakesh , Karthik S. , Kumar Abhishek , Tantri Adithya , Shahaji  , Sathvik S. 
Published: February 2025

Abstract
This study investigates the effects of strength and durability of concrete for different water-cement ratios, aggregate contents, and partial replacement of biomedical waste ash at 5%, 10%, 15%, 20%, and 25% by weight of cement. At 7, 14, and 28 days, the control mix showed inferior mechanical properties, particularly compressive strength, compared to concrete mixtures containing Biomedical Waste Ash (BWA). The replacement of cement by 5% and 10% increased the compressive strength but it is decreasing from 15%. Additionally, BWA modified concrete demonstrated a slower water absorption rate and minimal weight loss under acid test curing conditions, indicating enhanced durability. The economic and environmental benefits of incorporating biomedical waste into concrete promote sustainable construction practices. Using three machine learning approaches—K-Nearest Neighbors (KNN), Random Forest (RF), and CatBoost—the compressive strength of concrete with biomedical waste ash was simulated. Cement, biomedical waste, water absorption, slump, and the water-to-cement ratio were key input variables. Among the models tested, the RF model emerged as the most accurate, with a predictive performance of R2 = 0.9945 and RMSE = 0.7080. Its unparalleled reliability, consistency, and accuracy in predicting compressive strength make it a top choice for this task.
Decoding urban heritage: a multi-dimensional analysis framework applied to Assiut’s historic core
Authors: Refaat Ahmed Mohamed 
Published: February 2025

Abstract
One of the challenges confronting urban conservation is the inclusion of heritage areas in the development process. While often seen as constraints, heritage areas represent promising opportunities to enhance city character and quality. Nevertheless, the inclusion of heritage areas in the development process is intricate, requiring an extensive analysis of the urban form of heritage areas to facilitate the inclusion process. From this stance, this paper proposes a comprehensive analytical framework for identifying the features and characteristics of heritage areas, using Assiut City in Egypt as a case study. The proposed framework addresses four interrelated dimensions of urban design: the morphological, the functional, the perceptual, and the social. Space syntax methodology, mainly axial mapping, is employed to analyze the urban pattern of Assiut’s Historic Core to disclose its spatial configuration and morphological features. The author will use Survey and field observation to address the heritage area’s functional, perceptual, and social features (33 respondents and five interviewees). Thus, this paper utilizes a mixed-methods approach, combining quantitative and qualitative techniques to provide a holistic understanding of the urban heritage area. Implications of the study are discussed in terms of the importance of including heritage areas in the development process and how the proposed analytical framework can contribute to revealing the features of urban areas in general and heritage areas in particular. Thus, policymakers and urban planners can map out the areas of improvement and development fostering inclusive, well-integrated urban regeneration and urban development of our cities.
The relationship between commodity diversification and the adoption of technological innovations for Southeast beef cattle producers
Authors: Berg Nikolas L. , Thayer Anastasia W. , De Figueiredo Silva Felipe , Vassalos Michael , Wang Zuyi , Smith Nathan B. 
Published: February 2025

Abstract
For almost eight decades, productivity in the United States agricultural sector has substantially increased, in large extent due to the adoption of technological innovations. Despite the increased utilization of technology, questions remain regarding which producers are more likely to adopt a greater number of technological innovations. This research seeks to understand how commodity diversification strategies, farm characteristics, producer perceptions of risk, conservation, information sources, climate adaptation, and producer demographic characteristics are associated with technology adoption among beef cattle producers in the Southeast United States. Utilizing data from an online survey and an Ordered Probit model, we show that beef cattle producers who also produce fruit have an increased probability of adopting a greater number of technologies. The opposite effect is found for other commodities such as vegetables, row crops, and other livestock. Policy recommendations are also discussed.
Situations in which caregivers and patients are likely to collude: Perspectives from caregivers of advanced cancer patients in Bangladesh
Authors: Biswas Jheelam , Khanam Salma Ahsan , Tauhid Md. Shamsudduha , Sarker Shima Rani , Afsar Nahid , Islam Nashid 
Published: February 2025

Abstract

Objectives
This study aimed to explore situations where caregivers and patients are likely to collude, from the perspective of caregivers of advanced cancer patients in Bangladesh.

Methods
This study took place in 2 different tertiary care hospitals in Bangladesh. The study design included both quantitative and qualitative components. In this study, we focused on situations in which caregivers choose not to disclose the truth, regardless of their patients’ desire to know it. This may include instances of mutual withholding or cases of deliberate withholding by caregivers, even when patients express a desire to know the truth. While collusion may occur in some instances, not all situations qualify as collusion; nonetheless, all of these situations were broadly considered as collusion-prone. The intensity of enactment was assessed using the “Caregiver Collusion Questionnaire,” and in-depth exploration of collusion-prone situations was conducted through key person interviews with the caregivers.

Results
The intensity of enactment was medium to high among two-thirds (83.1%) of caregivers. This intensity was significantly associated with the caregivers’ relationship with the patients and their intention to disclose the truth (p < 0.01). Higher intensity of enactment has been observed among the children who are tending to their terminally ill parents. Four major themes regarding collusion-prone situation were generated by the qualitative analysis: (1) Reasons for nondisclosure; (2) Time of disclosure; (3) Selective disclosure; and (4) Discloser of truth.

Significance of Results
The nature of collusion-prone situations is shaped by culture and social values. By gradually and indirectly addressing these situations, healthcare professionals can assist families in navigating through difficult conversations and ensure that the patient’s wishes and values are respected.
Quantifying Biodiversity's Present and Future: Current Potentials and SSP‐RCP‐Driven Land Use Impacts
Authors: Cheng Yilin , Liu Hongxi , Du Jizeng , Yi Yujun 
Published: February 2025

AbstractBiodiversity, vital for ecosystem stability and human well‐being, faces threats from land use and climate change. Accurately predicting these effects is crucial for effective conservation. High emission development scenario is commonly viewed as the most detrimental to biodiversity. However, recent researches suggest a more complex relationship between development paths and biodiversity outcomes. Our study addresses this by using an emergy‐based approach to estimate current provincial‐level biodiversity potential and project future species richness losses (amphibians, mammals, and birds) across climate zones and provincial divisions under various SSP‐RCP scenarios for 2030, 2050, 2070, and 2090. The results revealed significant regional variations in China's biodiversity potential, with the highest in southwestern provinces. Future land use trends indicate increased construction land and barren alongside a decline in grasslands, leading to considerable habitat loss and fragmentation under various scenarios, stressing conservation needs. Future biodiversity loss follows the Hu line and climate zones, with significant decreases in the south and humid regions. Land‐use changes could reduce species richness by 1–6 per 10 km grid cell. High‐emission scenario SSP585 do not necessarily have the most detrimental effects on biodiversity and different scenarios require targeted focus on specific climatic zones and provinces. These findings underscore that different scenarios require targeted conservation efforts in specific regions sensitive to biodiversity loss. Our study provides a scientific foundation for these targeted efforts, ensuring that regions are prioritized under various future scenarios. This approach aids in developing effective conservation strategies amidst the complex interplay of land use dynamics and climate change.
Impact of Mid‐Pliocene Boundary Conditions on the Atlantic Meridional Overturning Circulation (AMOC)
Authors: Weiffenbach Julia E. , Chan Wing‐Le , Abe‐Ouchi Ayako , von der Heydt Anna S. 
Published: February 2025

AbstractWe use the coupled atmosphere‐ocean model MIROC4m to investigate the effect of mid‐Pliocene boundary conditions on the Atlantic Meridional Overturning Circulation (AMOC), studying the impact of increased , reduced ice sheets and altered orography and vegetation. We find that a higher concentration and smaller ice sheets both weaken the AMOC with respect to the pre‐industrial. The stronger mid‐Pliocene AMOC is therefore a consequence of mid‐Pliocene orography and vegetation, where the closed Arctic gateways are responsible for approximately 80% of the AMOC strengthening. The main mechanism for mid‐Pliocene AMOC strengthening is reduced transport of freshwater from the Arctic into the North Atlantic, enhanced by a decrease of surface freshwater flux into the high‐latitude North Atlantic.
Constraining 2.0 Ga Volcanism on the Moon via 40Ar/39Ar Dating of Chang'e‐5 Basalts
Authors: Su Fei , Zhang Xuhang , Li Youjuan , Jicha Brian R. , Joy Katherin H. , Li Qiuli , Chen Yi , Cai Shuhui , Liu Runchuan , Zhou Qin , Yang Saihong , Li Xianhua , Yang Liekun , Chen Wen , Li Junjie , Zhang Wanfeng , Qiu Huaning , He Huaiyu 
Published: February 2025

AbstractThe Chang'e‐5 landing site provides an important window into the Moon's late Eratosthenian period of volcanism at ∼2 Ga. Clarifying the Moon's history of volcanic activity using radioisotopic dating assists investigations of the evolution of the lunar surface as well as the Moon's internal dynamics. Recent chronological investigations of Chang'e‐5 basalts produced ages spanning ∼100 Ma, thereby inhibiting interpretation of the duration of volcanism recorded in the returned samples. We used microcomputed tomography and Back‐Scatter Electron imaging to characterize the structure and morphology of nine Chang'e‐5 basalt clasts. Several basalt clasts lack shock features and are interpreted to have not been significantly thermally disturbed. 40Ar/39Ar incremental heating produced well defined plateaus for four sub‐split samples that give a weighted mean age of 2,021 ± 17 Ma (2σ). These are among the youngest mare basalts to be dated thus far by the 40Ar/39Ar method and, when combined with most of the published Pb‐Pb ages for Chang'e‐5 basalts, define a single episode of mare volcanism at ∼2,021 Ma.
Investigating the Characteristics and Drivers of Slow Droughts and Flash Droughts: A Multi‐Temporal Scale Drought Identification Framework
Authors: Qi Zixuan , Ye Yuchen , Cai Yanpeng , Yuan Chaoxia , Xie Yulei , Cheng Guanhui , Zhang Pingping , Sun Lian , Wan Hang 
Published: February 2025

AbstractGlobal climate change has altered the characteristics of conventional drought events, with an increasing number of Slow droughts (SD) rapidly transitioning into Flash droughts (FD). This study introduces a novel multi‐temporal scale drought identification framework (MTSDIF) that classifies historical agricultural drought events into three types: SD, FD, and Slow‐to‐Flash Drought (SFD). Based on the MTSDIF, the GLDAS‐Noah root zone soil moisture dataset was used to analyze the spatiotemporal characteristics, evolution, and driving factors of multi‐temporal scale droughts in China. Our study confirms the effectiveness of the proposed MTSDIF in classifying droughts with different onset speeds (SD, FD, and SFD). The results indicate that, from 1980 to 2020, the three types of drought events in China exhibited short‐term, medium‐term, and long‐term periodic oscillations. Before 2000, SD events were the predominant type of agricultural drought in China, but post‐2000, the areas affected by FD and SFD have been continuously expanding. Compared to SD, key meteorological elements influencing FD and SFD show anomalies exceeding 0.5 times the standard deviation. In the southeastern regions of China, areas with human‐impacted soils, leached soils, and incept soils exhibit a higher response frequency to FD. Sea surface temperature indices, including the interannual El Niño‐Southern Oscillation in the Pacific and interdecadal variations such as the +PDO and −AMO, significantly influence the occurrence of FD in the monsoon regions of China (p < 0.01). Together, the results highlight the necessity of understanding the disparities and consistencies in land‐atmosphere‐ocean mechanisms behind drought events with varying onset speeds.
Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathway
Authors: Wu Hai-qi , Qin Ren-cai , Li Wei-jie , Liu Jie-na , Deng Chong , Zheng Zi-han , Zheng Jing-peng , Liu Yu , Meng Yan-fang , Tang Chun , Tan Hong-mei , Duan Fang-fang , Tang Yuan , Xiao Fan , Lu Li-wei , Dai Xiao-yan , Ma Kong-yang 
Published: February 2025

Abstract
Multiple myeloma (MM) is a prevalent hematologic malignancy characterized by abnormal proliferation of cloned plasma cells. Given the aggressive nature and drug resistance of MM cells, identification of novel genes could provide valuable insights for treatment. In this study we performed machine learning in the RNA microarray data of purified myeloma plasma cell samples from five independent MM cohorts with 957 MM patients, and identified O-GlcNAcylation transferase (OGT) and cell division cycle 27 (CDC27) as the key prognostic genes for MM. We demonstrated a close link between OGT and CDC27 in MM cells by knockdown of OGT with siOGT, pharmacological inhibition of O-GlcNAcylation with OSMI-1 and pharmacological accumulation of O-GlcNAcylation with Thiamet G. Using mass spectrometry and immunoprecipitation, we identified the O-GlcNAcylated CDC27 protein as a key target protein that may be directly downregulated by OSMI-1 in MM.1S cells. We further revealed that O-GlcNAcylation maintained CDC27 protein stability by blocking the autophagy-lysosome pathway (ALP). Moreover, we demonstrated the enhanced antitumor efficacy of combined OSMI-1 and bortezomib (BTZ) treatment in MM cells both in vivo and in vitro. Thus, this study identifies a novel function of O-GlcNAcylation-related ALP in regulating CDC27 protein stability and a potential therapeutic strategy for treating MM.
Nuclear localization of BRCA1-associated protein 1 is important in suppressing hepatocellular carcinoma metastasis via CTCF and NRF1/OGT axis
Authors: Xie Xiaoyu , Tsui Yu-Man , Zhang Vanilla Xin , Yu Tiffany Ching-Yun , Husain Abdullah , Chiu Yung-Tuen , Tian Lu , Lee Eva , Lee Joyce Man-Fong , Ma Hoi-Tang , Ho Daniel Wai-Hung , Sze Karen Man-Fong , Ng Irene Oi-Lin 
Published: February 2025

Abstract
Germline mutations of the deubiquitinase BRCA1-associated protein 1 (BAP1) lead to the “BAP1 cancer syndrome” characterized by development of cancers. However, the role of BAP1 in hepatocellular carcinoma (HCC) is unclear. We found that BAP1 was upregulated at mRNA level in human HCCs and significantly correlated with a more aggressive tumour behaviour. Intriguingly, we observed cytoplasmic but no or minimal nuclear BAP1 in human HCC samples by immunohistochemistry. We observed that, while BAP1 protein was found mainly in the cytoplasm and less in the nuclei of HCC cell lines, BAP1 expression was predominantly nuclear in HepG2 cells, by cell fractionation and immunofluorescence analyses. Functionally, in the orthotopic liver injection mouse model, silencing the BAP1 predominant nuclear expression of HepG2 cells promoted intrahepatic tumor metastasis, with more frequent tumor microsatellite formation and venous invasion. With transcriptomic profiling, we identified RHOJ amongst the downregulated targets in HepG2 cells upon BAP1 knockdown. Subsequent overexpression of RHOJ suppressed cell migration in HCC cells, suggesting that BAP1 might upregulate RHOJ resulting in reduced cell migratory ability of HCC cells. Furthermore, we identified two transcription factors, CTCF and NRF1, which activated BAP1 transcription by binding to BAP1 promoter region. On the other hand, we uncovered that O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) physically bound to BAP1 in the nucleus, resulting in diminished stability of the nuclear BAP1. Intriguingly, OGT transcription was upregulated and was also under the control of CTCF and NRF1 in human HCC, acting as a negative regulator of BAP1. To summarize, this study uncovered the underlying mechanisms of the regulation of BAP1 and that loss of the nuclear localization of BAP1 protein contributed to enhanced cell migration in vitro and more aggressive tumor behavior in human HCCs.
Cyclic jetting enables microbubble-mediated drug delivery
Authors: Cattaneo Marco , Guerriero Giulia , Shakya Gazendra , Krattiger Lisa A. , G. Paganella Lorenza , Narciso Maria L. , Supponen Outi 
Published: February 2025

Abstract
The pursuit of targeted therapies capable of overcoming biological barriers, including the blood–brain barrier, has spurred the investigation of stimuli-responsive microagents that can improve therapeutic efficacy and reduce undesirable side effects. Intravenously administered, ultrasound-responsive microbubbles are promising agents with demonstrated potential in clinical trials, but the mechanism underlying drug absorption remains unclear. Here we show that ultrasound-driven single microbubbles puncture the cell membrane and induce drug uptake through stable cyclic microjets. Our theoretical models successfully reproduce the observed bubble and cell dynamic responses. We find that cyclic jets arise from shape instabilities, as opposed to classical inertial jets that are driven by pressure gradients, enabling microjet formation at mild ultrasound pressures below 100 kPa. We also establish a threshold for bubble radial expansion beyond which microjets form and facilitate cellular permeation and show that the stress generated by microjetting outperforms previously suggested mechanisms by at least an order of magnitude. Overall, this work elucidates the physics behind microbubble-mediated targeted drug delivery and provides the criteria for its effective and safe application.
A novel microfluidic approach to quantify pore-scale mineral dissolution in porous media
Authors: Musabbir Rahman Rafid , Niemur Elliott , Blois Gianluca , Kazemifar Farzan , Kim Myeongsub , Li Yaofa 
Published: February 2025

Abstract
Mineral dissolution in porous media coupled with single- and/or multi-phase flows is pervasive in natural and engineering systems. Dissolution modifies the physical, hydrological, and geochemical properties of the solid matrix, resulting in a complex coupling between local dissolution rate and pore-scale flow. The work reports a microfluidic approach that includes 2D reactive porous media and advanced pore flow diagnostics for the study of pore-scale dissolution in porous media with unprecedented details. The 2D microfluidic porous media, called micromodels, were fabricated in calcite by combining photolithography and wet etching, which not only offers precise control over the structural and chemical properties, but also facilitate unobstructed optical access to the pore flow, significantly improving over existing methods. We believe the work represents the first of its kind as it for the first time directly applies photolithography to calcite samples and demonstrates the use of particle image velocimetry to investigate chemical reactions in porous media. The preliminary results have revealed the crucial roles of local concentration gradients in mineral dissolution and call for reconsideration of many assumptions used in the current modeling tools, which paves the way for renewed fundamental understanding of reactive transport and improved modeling tools with better accuracy.