bluuebunny/crossref_metadata_2025_split huggingface dataset:
A MR/PA Dual-Modality Imaging for quantitative evaluation of tumor vascular and immune microenvironment
Authors: Xu Nan , Wu Dan , Gao Jingyan , Jiang Huabei , Li Qinqing , Bao Shasha , Luo Yueyuan , Zhou Qiuyue , Liao Chengde , Yang Jun 
Published: November 2022

Abstract
Backgroud:
Tumor radiotherapy combined with immunotherapy for solid tumors has been proposed, but tumor vascular structure abnormalities and immune microenvironment often affect the therapeutic effect of tumor, and there are few reports on multimodal imaging markers in the study of tumor therapeutic response. The purpose of this study was to evaluate the dynamic monitoring of tumor blood vessels and microenvironment induced by radiotherapy by magnetic resonance imaging /photoacoustic imaging (MR/PA) imaging, and to explore the therapeutic effect of tumor radiotherapy combined with PD-L1 immunocheckpoint inhibitor.
Methods
The tumor-bearing mice (TBM) were randomly allocated to six groups: the low-dose group, high-dose group, low-dose + PD-L1 group, high-dose + PD-L1 group, anti-PD-L1 group and control group. Animals received 2 Gy/14 Fx (low-dose group) or 8 Gy/3 Fx (high-dose group) radiation and the combination treatment groups were given an anti-PD-L1 antibody for two consecutive weeks. MR/PA imaging was used to noninvasively evaluate the response of breast cancer model to different doses of radiotherapy, combined with histopathological techniques to observe the changes of tumor vessels and microenvironment.
Results
The inhibitory effect of high-dose radiotherapy on tumors was significantly greater than that of low-dose radiotherapy, with the MR images revealing that the signal intensity (SI) decreased significantly (p༜0.05). Compared with those in the other groups, the tumor vascular density decreased significantly, and the vascular maturity index (VMI) increased significantly in the low-dose group (p༜0.05). The PA images showed that the deoxyhemoglobin (HbR) and total hemoglobin (HbT) levels decreased and the SO2 level increased after radiation treatment. In addition, the high-dose group had an increased number of tumor-infiltrating lymphocytes (CD4+ T and CD8+ T cells) and natural killer (NK) cells and increased PD-L1 expression in the tumors (p༜0.05). The combination of radiotherapy and immunotherapy increased the survival rate of the mice, and a regimen of an 8 Gy dose of radiation combined with immunotherapy inhibited tumor growth and increased the survival rate of the mice to a greater degree than the 2 Gy radiation dose with immunotherapy combination (p = 0. 002).
Conclusion
Differential fractionation radiotherapy doses exert different effects on tumor vascular remodeling and the immune microenvironment, and MR/PA can be used to evaluate tumor vascular remodeling after radiotherapy, which is of great significance for the clinical applications of radiotherapy combined with immunotherapy.
Chitosan/Graphene Oxide Nanocomposite Coatings on Mg alloy: Corrosion and Biocompatibility Properties
Authors: Montazeri Arash , Hamghavandi Mohammad Ranjbar , Nezhadfard Mahla Sadat , Kari Ali Yeganeh 
Published: November 2022

Abstract
In the present study, optimal conditions for the fabrication of chitosan (CS)/graphene oxide (GO) nanocomposite coatings were evaluated by the pulse electrodeposition process (PED) on Mg–2wt%Zn scaffolds. The size distribution of CS, GO, and nanocomposite coatings were evaluated using dynamic light scattering (DLS). The coatings microstructure and morphologies were investigated by the Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV- visible), Raman spectroscopy, thermogravimetric analysis (TGA), derivative thermal gravimetric (DTG), differential thermal analysis (DTA), and scanning electron microscopy (SEM). Taguchi statistical method was used to optimize the effect of PED parameters, including peak current density (CD) and duty cycle (DC) as well as GO nanosheets content (1,2, and 3wt%). Results showed that optimal coatings were produced under the conditions of 2 wt% GO, CD = 20 mA/cm 2, DC = 0.5 and pH = 5. The process's time, temperature, and frequency were 20 min, 37oC, and 1000 Hz. The biocompatibility of coatings was assessed by in vitro test. The results of cell viability and adhesion of MG63 cells on optimal coating are promising for application in bone tissue engineering.
Adsorption Studies of Methylene Blue And Malachite Green Dyes From Aqueous Solution Using Calix[4]arene – Polyurethane
Authors: Ishak Syahirah , Rosly Nor Zida , Abdullah Abdul Halim , Ahmad Shahrul Ainliah Alang 
Published: November 2022

Abstract

Calix[4]arene-polyurethane (C4PU) polymer was used as an alternative adsorbent for the adsorption of methylene blue (MB) and malachite green (MG) dyes, from the aqueous solution. C4PU was synthesised by reacting p-tert-butyl calix[4]arene with hexamethylene diisocyanate (HMDI) as the cross-linking agent. A preliminary study was carried out to determine the best ratio of calix[4arene and HMDI in the adsorption of dyes and C4PU-4 shows a better adsorption compared to the synthesized C4PU in other ratios. The polymer was characterised by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), Brunauer-Emmett-Teller (BET) analysis and point of zero charge (pHPZC). The isotherms and kinetics of the adsorption of MB and MG were studied under a range of experimental conditions, including pH, adsorbent dosage, initial dye concentration, and contact time. The adsorption was determined by the adsorption percentage of MB and MG dyes from the solution. The adsorption process for both dyes is best described by the Langmuir isotherm model, and it follows the pseudo-second order kinetic model, with the maximum adsorption capacity (qmax) of MB and MG, respectively, was found to be 1.619 mg.g− 1 and 2.192 mg.g− 1.
Effect of melatonin on steroidogenesis-related enzyme expression and testosterone synthesis following CoCl 2 -induced hypoxia in TM3 Leydig cells
Authors: Karimi Shokooh , Jalili Cyrus , Mansouri Kamran , Bahremand Fariborz , Gholami Mohammad Reza 
Published: November 2022

AbstractHypoxia exerts detrimental effects on steroidogenesis and male fertility. Melatonin, a neuroendocrine hormone, modulates steroid production in mammals through its membrane receptors. This study examined the effects of melatonin treatment on steroidogenesis dysfunction and testosterone impairment following CoCl2-induced hypoxia in TM3 Leydig cells. The results indicated that CoCl2 treatment caused HIF1α overexpression in these cells. Moreover, CoCl2 treatment of TM3 Leydig cells led to considerable downregulation of StAR, 3β-HSD and GATA-4 as well as melatonin membrane receptors MT1 and MT2 mRNA/protein expression coupled with testosterone content repression in the cell culture medium. Melatonin administration inhibited HIF1α mRNA/protein expression, but had no significant effect on StAR, 3β-HSD, GATA-4, MT1 mRNA/protein expression or the testosterone level in the cell culture medium. Melatonin reduced the decrease in MT2 gene and protein expression following CoCl2-induced hypoxia in TM3 Leydig cells.
Governance of National Parks influenced by Local Community Participation: China's Potatso National Park
Authors: Ma Ting , Jia Lizhi , Zhong Linsheng , Gong Xinyu , Wei Yu 
Published: November 2022

Abstract
As a governance strategy, community participation is to implement the coordinated development of communities and protected areas. Therefore, the present study explored the community’s participation in the construction of Potatso National Park by means of a mixed-method approach that is derived from Ostrom’s (2007) socio-ecological systems and IUCN framework, calibrated to local residents’ self-assessments about the outcomes of community engagement on national park governance. From the six dimensions of ecological environment, livelihood assets, high-quality ecological products, management efficiency, social order and satisfaction that characterize the governance outcomes of protected areas, residents' attitudes and views on community participation in national park governance were analyzed. The results show that ecological environment has a positive impact on high-quality ecological products, on the contrary, livelihood assets are not significant; in addition, management efficiency has a significant positive impact on the ecological environment, livelihood assets, high-quality ecological products and social order. We concluded that community participation has a certain direct and indirect effect on the governance outcomes of Potatso National Park. It is suggested to continue to improve the community participation mechanism of residents from the two aspects of residents' subjective psychology and ecological compensation mechanism improvement. The transformation from the tourism development model to the national park is still in the process, and there existing quite a few problems. In the future, it is necessary to further realize the functions of national parks through institutional innovation, community participation capacity building, and the introduction of community institution participation.
Targeted next generation sequencing panel based screening and diagnosis of bone marrow failure cohort reveals diverse genomic landscape of pathogenic germline variants
Authors: BHATIA PRATEEK , Palla Swetha , Senguttuvan Gnanamani , Chhabra Prashant , Singh Minu , Khadwal Alka , Jain Arihant , Sharma Pankaj , Thakur Rozy , Sreedharanu Sreejesh , Bansal Deepak , Jain Richa , Peyam Srinivasan , Mohapatra Sonali , Jindal Ankur , Suri Deepti , Das Reena , Varma Neelam , Malhotra Pankaj , Trehan Amita 
Published: November 2022

Abstract
Introduction: Current study is a 4 year experience in diagnosis and screening of bone marrow failure cases using a targeted sequencing panel.
Methods: A total of 171 cases underwent targeted NGS and were categorized as suspected inherited bone marrow failure syndrome (IBMFS) group (106/62%) and idiopathic aplastic anemia (IAA) group (65/38%) based on clinical and laboratory criteria.
Results: 110 (64%) were pediatric (0-12 years) and 61 (36%) adolescent and adults (13-47 years). In suspected IBMFS group, 47 (44%) were positive for a pathogenic variation while in IAA group, 8 (12%) revealed a silent germline pathogenic variation. Whole exome sequencing performed in 15/61 suspected IBMFS group cases, revealed a clinically important variation in 3 (20%) cases. The targeted panel helped in establishing a diagnosis in 44% (27/61) of phenotypically heterogenous unclassified bone marrow failure syndrome cases and also led to amendment of clinical diagnosis in 5 (4.7%) of genotyped cases.
Conclusion: Overall targeted NGS panel yield was comparable to western data, however, this was achievable at 1/5th the cost, making it a cost-effective panel for resource constraint settings.
Evaluating the impact of pH on the growth and nutritional profile of Lemna minor L. (a sustainable alternative for Pakistan’s feed sector)
Authors: Ullah Hafiz , Gul Bakhtiar , Khan Haroon , Rehman Khushnood ur , Hameed Ishfaq , Zeb Umar , Roomi Sohaib , -E-Huma Zill 
Published: November 2022

Abstract
Pakistan's conventional feed supply is rapidly depleting, forcing the country to turn to non-conventional sustainable feed to meet the demand of the livestock and fishery sectors. The current study investigates the effect of pH on the nutritional composition of Lemna minor L., a freshwater macrophyte and a potential alternative feed. Fronds were grown in a pH range of 4–10 and replicated three times. The growth rate was highest (90 gm− 2 Day− 1) at pH 7 while lowest (40 gm− 2 Day− 1) at pH 4. Protein content dropped sharply at pH 4, but the highest (31g/100g) was found at pH 7 and 8. The lipid fraction was high at pH (9,10), while low in acidic. Carbohydrate was maximum (59.3 g/100g dw). A higher level of Ca (34, 37,38 mg/100g dw) was observed in pH 5 and 6 while low content (24 mg/100g dw) in the control group. In contrast, the Mg level slightly increased with increasing pH while the maximum concentration (32 mg/100g dw) was observed at pH10 and in the control group. High Fe moiety (936mg/100g) was found at pH 5 and 6. Elevated Mn (3.0mg/100g) was seen at a slightly acidic pH. The lowest level of Mn, 1.7 mg/100 and 2.0 mg/100 were recorded at pH 9 and 10 respectively. The highest fraction of Zn (0.08mg/100g) was observed in pH 6 and 7 while the lowest Zn came at pH 9. The study concluded that Lemna minor grows best between pH 6–10 under the agroclimatic conditions of Pakistan with optimal nutritional profile.
Halocynthiibacter halioticoli sp. nov., isolated from the abalone Haliotis discus hannai
Authors: Zhang Dan-Dan , Yue Yu-Yan , Zhang Meng-Di , Du Zong-Jun 
Published: November 2022

Abstract
A Gram-stain-negative, rod-shaped, glide, non-flagellated, and facultatively aerobic bacterial strain, designated as Z654T, was isolated from the gut of abalone Haliotis discus hannai from Rongcheng, Shandong province, China. Cells are 0.2–0.8 µm in width and 0.7–3.4 µm in length. Cells grew best at 30°C (range, 15–37°C), pH 7 (range, 6.0–8.5) and NaCl concentration of 2.0% (w/v) (range, 1–10%). According to the phylogenetic analysis of 16S rRNA gene sequence, the strain belongs to the genus Halocynthiibacter and the most close strain is Halocynthiibacter arcticus KCTC 42129T (97.12%). The genome size of strain Z654T was 3296250 bp and the DNA G + C content was 54.2 mol%. The average nucleotide identity (ANI) scores and digital DNA–DNA hybridization (dDDH) scores with H. arcticus KCTC 42129T was 70% and 14.6–18.2% respectively. The predominant quinone was Q-10 and the major fatty acids were C18:0, C18:1 ω7c 11-methyl and summed future 8. The polar lipids were consisted of phosphatidylcholine, phosphatidylglycerol, unidentified aminolipid and unidentifed lipids. Based on the phenotypic, phylogenetic and chemotaxonomic data, strain Z654T was considered to represent a novel species of the genus Halocynthiibacter, for which the name Halocynthiibacte halioticoli sp. nov., is proposed. The type strain is Z654T (= MCCC 1H00503T = KCTC 92003T).
Saccharomyces cerevisiae surface display of endolysin LysKB317 for control of bacterial contamination in corn ethanol fermentations
Authors: Lu Shao-Yeh , Liu Siqing , Patel Maulik , Glenzinski Kristina , Skory Christopher 
Published: November 2022

AbstractBackground Control of bacterial contamination in bioethanol fermentation facilities has traditionally relied on chemical-based products such as hop acids and the use of antibiotics. Recent emphasis on antibiotic stewardship has prompted new research into development of alternative approaches to microbial remediation strategies. We recently described a recombinant peptidoglycan hydrolase, endolysin LysKB317, that inhibitedLactobacillusstrains in corn mash fermentation. Here,Saccharomyces cerevisiaeEBY100 was used to anchor recombinant LysKB317 using cell surface display with the α-agglutinin proteins Aga1p-Aga2p. Results Immunostaining and confocal fluorescence were used for localization of the extracellular interface of the cells. Yeast surface expressed endolysin demonstrated an 83.8% decrease in bacterial cell counts compared to 9.5% decrease in control yeast. RecombinantS. cerevisiaeexpressing LysKB317 used for small-scale corn mash fermentation, when infected withLimosilactobacillus fermentum, showed the ability to proactively control bacterial infection for 72 hours with at least 1-log fold reduction. HPLC analysis of fermentation products showed improved ethanol concentration from 3.4% to at least 5.9% compared to infection-only control and reduced levels of lactic and acetic acid from 34.7 mM to 13.8 mM and 25.5 mM to 18.1 mM in that order. Conclusion In an optimized yeast surface display system, proactive treatment of bacterial contaminants by endolysin LysKB317 can improve fermentation efficiency in the presence ofL. fermentumcontamination.
Sodium thiosulfate ameliorates atopic dermatitis symptoms via inhibiting inflammatory infiltration and restoring skin barrier function
Authors: Zeng# Yilan , Wang# Dan , Liu Yuanhong , Tang Ziting , Zong Xiule , Li Xuemei , Yang Shengbo , Lu Jianyun 
Published: November 2022

Abstract
Background
Atopic dermatitis (AD) is a common disease with a considerable impact on the affected individual’s quality of life and has limited treatment options. Sodium thiosulfate (STS) is a traditional medicine used in the rescue of cyanide poisoning, and some pruritus dermatosis. However, the exact efficacy and mechanism of its application with AD are not clear.
Patients and Methods:
We reviewed the records of patients with moderate to severe AD treated in the department of dermatology, the Third Xiangya Hospital, between January 2020 and July 2021. The change of Eczema Area and Severity Index (EASI), Scoring of Atopic Dermatitis index (SCORAD), Atopic Dermatitis Control Tool (ADCT), Patient-reported outcomes (PROs), skin barrier indexes and serum biochemical indicators were recorded.
Results
A total of 60 moderate to severe AD patients were enrolled, 20 in the STS 0.64g once daily + conventional therapy (STS QD) group, 20 in the STS 0.64g twice daily + conventional therapy (STS BID) group and 20 in the conventional therapy (control) group. Conventional therapy consisted of intravenous fluids of calcium, vitamin C and oral antihistamines rupatadine and bepotastine. Treatment with STS led to greater improvement with higher proportion of EASI50 and EASI75 and lower ADCT index compared to the control group. After treatment, greater improvement in PROs, skin barrier indexes were also observed in the STS treatment group than in the control group. To further study the underlying mechanism of STS, we analyzed the serum biochemical indicators. STS downregulated IgE by 4.12- and 7.26-folds (P = 0.0006 and P < 0.0001, respectively) and eosinophils by 2.24- and 5.28-folds (P = 0.0205 and P < 0.0001, respectively) in STS QD and STS BID group. In addition, STS downregulated interleukin-13(IL-13) by 2.86- and 3.16-folds (Both P < 0.0001) and interleukin-4 (IL-4) by 2.42- and 4.68-folds (Both P < 0.0001) in STS QD and STS BID group.
Conclusion
STS in combination with conventional therapy improves the signs and symptoms of AD by improving skin barrier function and downregulating concentrations of IgE, eosinophils and release of IL-4 and IL-13.
Functional Epiphora – An under-reported entity
Authors: Usmani Eiman , Shapira Yinon , Selva Dinesh 
Published: November 2022

Abstract

Purpose To determine the etiology of epiphora in a tertiary Australian lacrimal clinic and highlight the high proportion of ‘functional’ cases Methods Single-center retrospective review. Records of adult patients presenting to a tertiary lacrimal clinic from January 2011 to February 2021 with epiphora were reviewed. Patients underwent testing with syringing/probing and lacrimal imaging to reach a diagnosis of functional epiphora. Functional epiphora was diagnosed based on the exclusion of alternate causes of epiphora on clinical examination, patent lacrimal syringing, normal dacryocystography, and delay on dacryoscintigraphy Results 576 symptomatic eyes of 372 adult patients (mean 66.2 ± 15.5years, 63.4% females) with epiphora were evaluated for causes. Post-sac obstruction (stenosis/complete obstruction) and functional epiphora (non-anatomical delay) were the most common causes of presentations to the lacrimal clinic (26% each). Functional epiphora with post-sac delay was substantially more common than functional epiphora with pre-sac delay (89% versus 11%). In 16% of the cases, no cause for the epiphora was found while more than one cause (multifactorial) was present 11% of the time. Conclusion Functional epiphora was found to be as common as a nasolacrimal anatomical obstruction when lacrimal imaging is utilized.
Prevalence and associated factors of alcohol consumption among pregnant women attending antenatal care in a Rural District in Tete, Mozambique
Authors: Xavier Sancho Pedro , José Jorge Manuel , Cote Nelson Domingos , Xavi Ramim , Victor Audêncio 
Published: November 2022

Abstract
Background: Alcohol consumption during pregnancy is a significant public health and pregnancy-related problem, such as spontaneous abortion, stillbirth or low birth weight. According to the WHO, there is no safe amount of alcohol consumption during pregnancy. Studies show high rates of alcohol consumption during pregnancy in sub-Saharan African countries with a prevalence range (2.2-87%). This research aimed to determine the prevalence and associated factors of alcohol consumption among pregnant women attending antenatal care in a rural district of Tete, Mozambique.
Methods: A cross sectional survey was conducted between May and June 2022. The non-probability convenience method was applied for sample selection. Prevalence was determined using a 95% CI. binary logistic Regression was used to determine the factors associated with alcohol consumption. Data were analysed using statistical SPPS v26 and Stata.
Results: The prevalence of alcohol consumption during pregnancy was 85.9% (152/177; 95% CI: 80.7-91.0), which was associated with increasing age (OR= 8.86; 95% CI: 2.646-29.687; p-value = 0.000 and OR= 9.81; 95% CI: 2.262-42.576; p-value = 0.002) respectively. In addition, another associated factor was following some religion (OR= 0.07; 95% CI: 0.020-0.246; p-value = 0.000).
Conclusion: The survey significantly reveals a high prevalence of alcohol consumption among pregnant women attended in a antenatal care in Chitima. Maternal age and religion were the variables that have some significant association with alcohol consumption during pregnancy. Therefore, interventions for behavioural change to reduce alcohol consumption during pregnancy are needed, especially in all age groups.