bluuebunny/crossref_metadata_2025_split huggingface dataset:
Degree of Gelatination on Ag-Nanoparticles to Inactivate Multi-drug
Resistant Bacterial Biofilm Isolated from Sewage Treatment Plant
Authors: Girigoswami Agnishwar , Saraswathi Nagaraj , Girigoswami Koyeli , Divya Kurunchi Chellapathi , Kumar Subbaraj Gowtham 
Published: May 2022

Introduction:
Overuse and improper dosage of antibiotics have generated antimicrobial
resistance (AMR) worldwide. Pseudomonas aeruginosa (PA), a well-known bacterial strain can establish
MDR leading to a variety of infections in humans. Furthermore, these PA strains hold the ability to
form biofilms by generating extracellular polymeric substances on the surface of medical tools and
critical care units. To supersede the infectious effect of MDR organisms, silver nanoparticles have been
known to be the choice.

Materials and Methods:
Hence, the present study concentrates on the engineering of varying concentrations
of gelatin-based polymeric hydrogel embedded with silver nanoparticles (G-AgNPs) for controlled
bactericidal activity against MDR PA biofilms. Biofilms formation by MDR PA was confirmed
microscopically and spectroscopy was taken as a tool to characterize and analyze the efficacy at every
stage of experiments.

Results:
When MDR PA biofilms were treated with G-AgNPs prepared with 5 % gelatin concentration
(AgNP3), they exhibited superior bactericidal activity. Furthermore, a dose-dependent study showed
that 800 nM of AgNP3 could inhibit the growth of MDR PA.

Conclusion:
Hence it can be concluded that silver nanoparticles synthesized in the presence of 5%
gelatin can act as a bactericidal agent in the inactivation of MDR PA biofilms, thereby controlling the
infections caused by these biofilms.
Wound Healing Properties of Pelargonium Graveolens L'Hér Extract
Lipogel: In-Vivo Evaluation in an Animal Burn Model
Authors: Ebrahimnejad Pedram , Farmoudeh Ali , Asgarirad Hossein , Tehrani Bardia Berenji , Azad bakht Mohammad , Davoodi Ali , Rezaeiroshan Anahita , Hosseini Seyyedeh Saba 
Published: May 2022

Background:
Pelargonium graveolens L'Hér has traditionally been used to reduce skin inflammation,
and recent studies have confirmed antioxidant compounds in the plant's extract. The present
study aimed to prepare a lipogel formulation from P. graveolens hydroalcoholic extract and evaluate
its efficacy on the wound healing process in an animal model.

Material and Methods:
The aerial part extract of P. graveolens was prepared through percolation. Additionally,
plastibase was prepared by mixing 5% of low-molecular-weight polyethylene with hot mineral
oil (130°C). The extract (5%) was levigated in the mineral oil (5-15%) and dispersed in the cooled
plastibase. The physical properties of the lipogel, thermal stability, and microbial limits were tested.
Further, the effect of the lipogel in the wound healing rate was examined among male Wistar rats, and
skin tissue samples were assessed histologically.

Results and Discussion:
The results represented the best rheological and thermal stability characteristics
in the formulation with 5% mineral oil (as the levigator). The lipogel-treated group had the least
burn area compared to the silver sulfadiazine and negative control groups (p<0.05). The microscopic
examination of tissue samples revealed increased collagen fiber production and maturation and significantly
also faster epithelial repair among lipogel-treated rats than in the other two groups(p<0.05).

Conclusion:
The results indicated the significant therapeutic effects of P. graveolens lipogelon burn
healing. The suitable physicochemical properties and the low lipogel production cost facilitate further
scale-up studies.
Nasal Caffeine Thermo-Sensitive In Situ Gel for Enhanced Cognition
after Sleep-Deprivation
Authors: Ma Shan , Li Liqin , Du Lina , Zhu Siqing , Zhang Yuanyuan , Li Qi , Pang Lulu , Ma Jinqiu , Wang Chunqing , Zhang Shouguo , Jin Yiguang , Wang Xiu 
Published: May 2022

Background:
Caffeine abundant in coffee has a strong excitation effect on the central nerve
system (CNS).

Methods:
To combat the adverse effects of sleep deprivation on physical and mental health, this article
designed a new nasal temperature-sensitive gel loaded with caffeine, whose effects of awakening and
improving cognition in sleep-deprived rats were evaluated.

Results:
It was found that the caffeine thermo-sensitive in situ gel (TSG) stayed in the nasal cavity for a
longer time and increased the contact time between the drugs and the nasal mucosa, which made it possible
for caffeine TSG to exert a lasting effect. Secondly, compared with sleep-deprived rats, those administrated
with caffeine TSG were more responsive in behavioral experiments. Moreover, the antipentobarbital
test proved that caffeine TSG could prolong the sleep latency and shorten the sleep time.
Furthermore, caffeine TSG could significantly restore the cognitive ability by ameliorating neuronal cell
injuries by upregulating brain-derived neurotrophic factor (BDNF) levels.

Conclusion:
Generally, caffeine TSG could quickly exert the efficacy of enhancing cognition and wakefulness,
and overcome the drawbacks of frequent medications. It can potentially be used for the treatment
of psychiatric disorders, such as dementia, Parkinson and Alzheimer’s disease.
Graphite Oxide as a Catalyst for Dehydration of Alcohols
Authors: Xu Liang , Yan Peng , Zhao Guangzhou , An Pengsheng 
Published: May 2022

Background:
The methods of dehydration of alcohols frequently suffer from the following
drawbacks: high reaction temperature, toxic catalysts, high catalyst loading, and difficulty to remove catalysts.
Therefore, the development of a new catalyst for the dehydration of alcohols is still of importance.

Methods:
Graphite oxide is used as a catalyst for dehydration of alcohols.

Results:
Graphite oxide is used as a catalyst for dehydrating tertiary and secondary alcohols to the corresponding
alkenes as well as dehydrating primary alcohols and diols to the corresponding ethers and cycloethers
in moderate to excellent conversion rates and good selectivity.

Conclusion:
In these reactions, GO prepared by the improved Hummers method showed high catalytic
activity. As an efficient catalyst, GO is easily available, cheap, weakly acidic with low toxicity, and well
tolerant to various functional groups.
Adsorption Properties and Quantum Molecular Descriptors of the Anticancer
Drug Cytophosphane on the Armchair Single-Walled Carbon
Nanotubes: A DFT Study
Authors: Felegari Zahra , Hamedani Shahla 
Published: May 2022

Abstract:
In the current work, the adsorption of cytophosphane (cytophosphane is a chemotherapeutic drug and is used to treat several specific autoimmune diseases and malignant processes) on the (5,5) SWCNT was studied using density functional theory (DFT) calculations in terms of geometry, energy gap, charge transfer, molecular electrostatic potential surface, and density of state analysis. The behavior of the binding properties and the electronic structures revealed that the cytophosphane molecule could be adsorbed on the SWCNT by the adsorption energy of approximately -100.3 kcal mol-1. Also, it was found that the electronic properties of the SWCNT are very sensitive to the presence of cytophosphane molecules so that the energy gap of the nanotube is changed about 61% after the adsorption process. Based on calculated results, the SWCNT is expected to be suitable as a drug carrier for the delivery of cytophosphane drug.
Bioinformatic Screening of Compounds from Iranian Lamiaceae Family Members against SARS-CoV-2 Spike Protein
Authors: Alibakhshi Abbas , Gharibi Shima , Ahangarzadeh Shahrzad , Yarian Fatemeh 
Published: May 2022

Background:COVID-19 (coronavirus disease 2019) is still a major challenge worldwide. The disease is caused by binding the coronavirus to ACE2 receptors on lung cells, infecting the cells and triggering the onset of symptoms. The prevention of such a binding in which the virus is eventually unable to enter the cell could be a promising therapeutic approach.Methods:In this in silico study, 306 compounds of Lamiaceae family native in Iran (native Mints) were retrieved from several databases as 3D structures, and after that molecular docking and virtual screening, the compounds with inhibitory potential were selected in terms of free energy binding against the spike protein of the virus. The pharmacokinetic profile of selected compounds was evaluated, and by molecular dynamic simulation and MM/PBSA, four compounds were further assessed for binding affinities against the receptor-binding domain of the spike.Results:The results showed the Catechin gallate and Perovskone B from Stachys and Salvia genus generated a stronger binding affinity, and therefore could act as potential inhibitory compounds of RBD of the SARS-CoV-2 spike protein.Conclusion:This study revealed that some members of the Lamiaceae family could be employed to inhibit SARS-CoV-2 activity through interaction with spike protein and therefore could be used for further investigation in vitro and in vivo.
Design and Prediction of ADME/Tox Properties of Novel Magnolol Derivatives
as Anticancer Agents for NSCLC Using 3D-QSAR, Molecular Docking,
MOLCAD and MM-GBSA Studies
Authors: Daoui Ossama , Elkhattabi Souad , Chtita Samir 
Published: May 2022

Introduction:
In this work, we used several molecular modeling techniques to design new
molecules for the treatment of non-small cell lung cancer (NSCLC).

Methods:
For this purpose, we applied 3D-QSAR, molecular docking, MOLCAD, ADMET, and
MMGBSA studies to a series of 51 natural derivatives of magnolol.

Results:
The developed models showed excellent statistical results (R² = 0.90; Q² = 0.672; R²pred = 0.86)
for CoMFA and (R² = 0.82; Q² = 0.58; R2
pred = 0.78) CoMSIA. The design of eleven new molecules was
based on predictions derived from the 3D-QSAR model contour maps, molecular docking and MolCAD
analyses. In silico drug-like and ADMET properties studies led to the selection of four new molecules
designed as potential agents for NSCLC therapy. Molecular docking and MM-GBSA simulations of proposed
structures with EGFR-TKD (PDB code: 1M17) showed that ligands X10 and 30 attained better
stability in the 1M17 protein pocket compared to the Erlotinib ligand used as a reference.

Conclusion:
Incorporating all the molecular modelling techniques used in this work is conducive to the
design of new molecules derived from the 3-(4-aminobipyridin-1-yl)methyl structure of magnolol, a candidate
for drug design for the treatment of non-small cell lung cancer. Therefore, the molecular structures
(X10 and 30) can be proposed as a key to designing new drugs against NSCLC.
Systematic Review: Association of Pesticide Exposure and Child Wheeze
and Asthma
Authors: Gilden Robyn C. , Harris Ryan L. , Friedmann Erika J. , Han Myeunghee , Hackney Alisha J. , Olorunyemi Emmanuel , Spanier Adam J. 
Published: May 2022

Background:
The prevalence of wheeze and asthma has risen over recent decades for all age groups, especially children. These disorders can lead to decreased quality of life, missed school, urgent care and emergency department visits, hospitalizations, and increased health care costs. En-vironmental exposures, including pesticide exposure, are likely a contributing factor to this in-creased prevalence.

Objective:
To evaluate the association of pesticide exposure with childhood wheeze and asthma.

Methods:
We conducted a systematic review evaluating studies of pesticide exposure (measured objectively) and child respiratory outcomes. We searched PubMed, Embase (Elsevier), CINAHL (EBSCO), Scopus (Elsevier), Cochrane Database of Systematic Reviews (Wiley), and ClinicalTri-als.gov from 1988 - 2021. Main search keywords included “pesticides”, “insecticides”, “herbi-cides”, “respiratory”, “asthma” and “wheeze”.

Results:
Out of 5767 studies, 25 met the inclusion criteria; eight evaluated prenatal pesticide expo-sure (n=8407), twelve evaluated postnatal exposures (n= 50,488), and five evaluated pre-and post-natal exposures (n=20,919). Main pesticides investigated were dichlorodiphenyldichloroethylene (DDE) (14 studies) followed by organophosphates (7 studies). Primary methods of outcome as-sessment were questionnaire-based (84%), followed by spirometry (16%), registry data, and blood measures. Studies varied in the strength of evidence relating to study design and measures. Most studies (84%) reported a positive association of exposure with adverse child respiratory health.

Conclusion:
The studies suggest an association of pesticide exposure and childhood wheeze and asthma. The varying results and methods reinforce the need for more research and standardized ap-proaches to these studies to confirm the suggested association of pesticide exposure and childhood wheeze and asthma.
Persistent/Late Complications of COVID-19 in Affected Emergency Medical
Technicians: A Case Series and Brief Literature Review
Authors: Baratloo Alireza , Saberian Peyman , Pazooki Behshad , Shajari Zahra , Hasani-Sharamin Parisa , Jamshididana Mahnaz , Karimi Somayeh 
Published: May 2022

Background:
The present study aimed to assess the prevalence of persistent/late compli-cations after recovery from the acute phase of COVID-19 in emergency medical technicians (EMTs).

Methods:
This is a cross-sectional case-series study performed during the last quarter of 2020 in Tehran, Iran. All EMTs who had been diagnosed with COVID-19 were eligible. The researcher contacted the EMTs via telephone to determine any complications following their recovery. Those who suffered from any complication were referred to an internal specialist physician for a detailed history and physical examination. Based on the physician’s opinion, some paraclinical or clinical evaluations were requested to be performed.

Results:
Four hundred thirty-one confirmed cases and two deaths due to this disease were registered among the Tehran EMS center’s EMTs during the study period. Two hundred thirty-eight EMTs were contacted, and 22.7% of them had at least one persistent/late complication following recovery of the acute phase of COVID-19; of whom, 28 EMTs were visited by an internist and completed the tests. The final participants mentioned seventy-five persistent/late complications. Only one EMT had a residual lesion among those who underwent lung CT scans. There were also some pathologi-cal findings in the echocardiographic examination and spirometry.

Conclusion:
Our study showed that persistent/late-onset complications could likely accompany by COVID-19.
A Systematic Review of Instruments Measuring the Division of Care
Responsibilities between Children with Type 1 Diabetes and their Parents
Authors: Aalders Jori , Nefs Giesje , Hartman Esther , A. Nguyen Linh , Winterdijk Per , van Mil Edgar , Aanstoot Henk-Jan , Pouwer Frans 
Published: May 2022

Background:
The division of care responsibilities between parents and children with
type 1 diabetes, and an optimal transfer of responsibilities from parent to child over time are assumed
to be key for optimal diabetes outcomes during childhood and adolescence. However, an
overview of instruments assessing this division as well as their psychometric qualities is currently
lacking.

Objective:
The study aims to 1) identify all existing instruments, 2) evaluate their psychometric
properties, and 3) provide an overview of scoring methods.

Methods:
Pubmed and PsycINFO were searched using a priori-defined search string. Peerreviewed
studies in English using an instrument assessing the division of diabetes care responsibilities
between children (6-18 years) and parents were included. In total, 84 of 725 articles qualified,
covering 62 unique samples.

Results:
Thirteen questionnaires were identified. The Diabetes Family Responsibility Questionnaire
(DFRQ) was most frequently used across studies. Instructions, content and number of tasks,
response options, and scoring methods varied across questionnaires. Recent studies often adapted
questionnaires, contributing to the heterogeneity across measures. Overall, reporting and quality of
psychometric properties was suboptimal.

Conclusion:
The division of diabetes care responsibilities can be operationalized with various instruments,
each having its strengths and weaknesses but all with limited psychometric support. To
measure the division of diabetes care responsibilities more adequately, an updated version of the
popular DFRQ or a new scale needs to be developed and evaluated.
Traditional Use, Phytochemistry and Pharmacology of Ananas comosus
(L.) Merr.(Family Bromeliaceae): An Update
Authors: Kumar Virender , Dureja Harish , Garg Vandana 
Published: May 2022

Background:
Ananas comosus belongs to the family Bromeliaceae and commonly known as pineapple, anannas and anannaasa.

Objective:
The present review gives detailed information about synonyms, geographical distribution, taxonomical classification, cultivation, pharmacognostical and phytochemical profile as well as about therapeutic potential of Ananas comosus.

Methods:
For the preparation of manuscript, all the information was collected from scientific engines and databases (Web of Science, Scifinder, Google Scholar, Scopus, PubMed, Wiley Online Library, Taylor & Francis online) using different keywords.

Result:
Recent studies of A.comosus are based on traditional use, phytochemistry and pharmacology. The nutritional properties of the fruit are well known. The fruit contains various biological active phytoconstituents such as polyphenol compounds, volatile compound and carotenoids etc. which are therapeutically active. A.comosus is used for digestive issues, inflammation, in sea sickness and in a sore throat. Different studies on A.comosus have been conducted due to ethanopharmacological profile. It possesses antimalarial, antidiabetic, abortifacient, anticancer, antioxidant and antidiarrhoeal activity etc. Finally Industrial applications and ongoing clinical trials on pineapple are also summarized.

Conclusion:
This review would assist researchers in formulating a therapeutically active herbal remedy.
Rapid and Precise Discrimination between Pure and Adulterated
Commercial Indian Honey Brands using FTIR Spectroscopy and Principal
Component Analysis
Authors: Singh Bipin , Barman Sanmitra 
Published: May 2022

Background:
Four leading commercial Indian honey brands were investigated using
FTIR spectroscopy and principal component analysis for rapid and accurate differentiation of
pure, mildly adulterated, and highly adulterated honey brand samples. We examined the ability
of PCA on second derivative of FTIR data to descriminate between the pure and adulterated
commercial honey brands.

Methods:
This study is the first to investigate commercial Indian honey brands using FTIR and
PCA. We show that the top two principal components could easily descriminate between the
pure and adulteraated honey brands.

Results:
Hence such methods can investigate adulterations in bulk commercial honey brand
samples where sophisticated instrumentations and facilities are not available.

Conclusion:
Thus, the potential of FTIR and PCA can be further explored for detecting the presence
of adulterations in bulk honey samples without much cost and effort.
Reversed Potts Shunt Outcome in Suprasystemic Pulmonary Arterial
Hypertension: A Systematic Review and Meta-Analysis
Authors: Mendel Brian , Christianto Christianto , Angellia Phoniex , Holiyono Indra , Prakoso Radityo , Siagian Sisca Natalia 
Published: May 2022

Background:
Reversed Potts shunt has been a prospective approach to treat suprasystemic
pulmonary hypertension, particularly when medication treatment fails to reduce right ventricular
afterload.

Objective:
This meta-analysis aims to review the clinical, laboratory, and hemodynamic parameters
after a reversed Potts shunt in suprasystemic pulmonary hypertension patients.

Methods:
Six electronic databases were searched from the date of inception to August 2021, where
the obtained studies were evaluated according to the PRISMA statement. The effects of shunt creation
were evaluated by comparing preprocedural to postprocedural or follow-up parameters, expressed
as a mean difference of 99% confidence interval. Quality assessment was conducted using
the STROBE statement.

Results:
Seven studies suited the inclusion criteria which were included in this article. A reduction
in upper and lower limb oxygen saturation [Upper limb: St. Mean difference -0.55, 99% CI -1.25
to 0.15; P=0.04; I2=6%. Lower limb: St. Mean difference –4.45, 99% CI –7.37 to –1.52;
P<0.00001; I2=65%]. Reversed Potts shunt was shown to improve WHO functional class, 6-minute
walk distance, NTpro-BNP level, and hemodynamic parameters including tricuspid annular plane
systolic excursion, interventricular septal curvature, and end-diastolic right ventricle/left ventricle
ratio.

Conclusion:
Reversed Potts shunt cannot be said to be relatively safe, although it allows improvement
in the clinical and functional status in patients with suprasystemic PAH. Reversed Potts shunt
procedure may be the last resort for drug-resistant pulmonary hypertension as it is considered a
high-risk procedure performed on patients with extremely poor conditions.
A Review of Spectroscopic and Non-Spectroscopic Techniques for Diagnosing
Breast Cancer
Authors: Isabella S. Stella Jenifer , Sunitha K.A. , Arjunan Sridhar P. , Pesala Bala 
Published: May 2022

Abstract:
Malignancy is one of the consequent driving explanations behind death worldwide, and it is assessed at 9.6 million in 2018. Around 1 out of 6 deaths are the direct result of the malignancy. Clinicians state, that age and breast density are two natural elements resulting in the danger of cancer. The mortality rate brought about by malignant growth in low and focus pay countries is, for the most part, around 70%. Imaging techniques play a vital role in detection, staging, and help in guiding treatment decisions. This review paper presents a comprehensive survey involving a literature study about the evolution and efficacy of various breast cancer detection techniques. This work studies the various procedures of imaging techniques mammograms, ultrasound, MRI, PET, CT, Terahertz Spectroscopy, Raman Spectroscopy, Optical coherence Tomography, Mass spectroscopy, diffuse reflectance spectroscopy and Infrared Thermography. Since cancer is a complicated illness with diverse pathophysiology, numerous modifications of the fundamental detection approach employed in each of these modalities have been performed throughout the years to increase the detection efficiency. This paper covers basic preliminary result with FFPE breast cancer blocks of malignant and normal subjects using THz Techniques that lay as a proof of a concept to carry out further research.