bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production performance and rumen bacterial community structure of Hu sheep fed fermented spent mushroom substrate from Pleurotus eryngii
Authors: Huang Xiaoyun , Zhou Liuting , You Xiaofeng , Han Haidong , Chen Xinzhu , Huang Xiusheng 
Published: December 2022

Abstract
This study aimed to compare the slaughter performance, meat quality and rumen bacterial community structure of Hu sheep by adding raw materials( RL0), 0 (RL1), 15% (RL2), 30% (RL3) and 45% (RL4) fermented spent mushroom substrate from Pleurotus eryngii to the basal diet. The results showed that: (1) The pre-slaughter weight (PSW), carcass weight (CW) and slaughter rate (SR) of RL2 group were higher than others, but the differences between the groups were not significant (p > 0.05). (2) The contents of threonine, valerine, leucine, lysine, histidine, total essential amino acids, aspartic acid, serine, glutamic acid and arginine of the musculi longissimus thoracis in RL2 and RL3 groups were significantly higher than RL1 and RL4 (p < 0.05). (3) A total of 1202445 valid sequences were obtained from rumen of Hu sheep fed different amounts of fermented feed, and the valid sequences were clustered into 9824 Operational Taxonomic Units (OTUs). (4) α diversity analysis showed that the richness and diversity of rumen bacterial communities in Hu sheep in RL1, RL2, RL3 and RL4 groups were significantly higher than RL0 group (p < 0.05). β diversity analysis showed that the bacterial community structure was the most different between RL0 and RL3. (5) At the genus level, compared with RL1, the relative abundance of Christensenellaceae R-7 in RL3 group decreased significantly by 33.59%, the relative abundance of Prevotellaceae UCG001 in RL2, RL3 and RL4 decreased significantly by 50.41%, 62.24% and 49.17%, respectively, and the relative abundance of Ruminococcaceae NK4A214 in RL2 group increased significantly by 35.01% (p < 0.05). In summary, the addition of fermented Pleurotus eryngii mushroom bran to the basal diet of Hu sheep can significantly improve the slaughter performance, meat quality and rumen bacterial community diversity and abundance of Hu sheep.
In vitro comparative evaluation of polycaprolactone/polylactic acid copolymer and expanded polytetrafluoroethylene based Guided Tissue Regeneration membrane for bacterial adhesion
Authors: Sareen Shubham , Bey Afshan , Zia Afaf , Khan Fatima 
Published: December 2022

Abstract
Purpose: Earlier in vitro models have aimed to study the barrier function against the bacterial penetration across the GTR membranes. No such study is present in the literature which is aimed to quantify bacterial adhesion over the surface of the membrane irrespective of surface being examined.
Method: Sterile Resorbable PLA/PGA copolymer and Non resorbable Microporous eptfe based GTR membrane were used in the study. Both groups were subdivided into two subgroup (n=10) based on incubation period of 24 and 48 hours. Incubated in Todd Hewitt broth with streptococcus mutans, at each incubation time period the samples were vortexed and analysed for bacterial count (CFU) and also by scanning electron microscopy.
Result: Between PLA/PGA and ePTFE GTR membrane at 24 hrs incubation time period , there was a significant difference in terms of Colony forming units (W = 100.000, p = <0.001), with the median Colony forming units being highest in the PLA/PGA GTR membrane. Also at 48 hrs incubation time period, There was a significant difference in terms of Colony forming units (W = 100.000, p = <0.001), with the median Colony forming units being highest in the PLA/PGA gtr membrane.
Conclusion: Our findings depicts that ePTFE based GTR membrane showed significantly lesser bacterial adhesion on its surface as compared to PLA/PGA at both the incubation periods i.e. 24hrs and 48 hrs as shown by both spot test and SEM .
Seroprevalence of rubella hemagglutination inhibition and rubella-specific IgM antibodies and the significance of rubella-specific IgM antibody measurement in pregnant Japanese women: A retrospective study
Authors: Hiiragi Kazuya , Shindo Ryosuke , Shinoda Satoru , Okuda Mika , Miyagi Etsuko , Aoki Shigeru 
Published: December 2022

Abstract
Background
Cases of congenital rubella syndrome are reported annually in Japan. Rubella-specific IgM antibody titers can show false positivity or remain positive for a long period. Furthermore, the seroprevalence of rubella hemagglutination inhibition (HI) and IgM antibodies is unknown, and the relevance of measuring rubella-specific IgM antibodies in asymptomatic individuals is unclear. Hence, this study aimed to assess the validity of rubella-specific IgM antibody titers as a diagnostic marker, by retrospectively assessing the prevalence of rubella HI and IgM antibodies among pregnant Japanese women. The study also aimed to estimate the incidence of rubella among rubella IgM antibody-positive pregnant women.
Methods
This single-center, retrospective study measured rubella HI and IgM antibody titers of pregnant Japanese women during prenatal visits from January 2007 to December 2020 at Yokohama City University Medical Center Perinatal Center for Maternity and Neonates. Rubella-specific IgM antibody titers were measured by enzyme immunoassay. Values > 1.2 and ≤ 1.2 were defined as positive and negative, respectively.
Results
A total of 14965 pregnant women were included in the study, of whom 186 (1.2%) were IgM-positive. One patient (0.5%) was clinically diagnosed with rubella. Of the IgM-positive patients, 136 (73.1%) had rubella HI antibody titers of < 1:256. The patient with clinically diagnosed rubella had a fever and skin rash and showed a high titer value for both rubella HI (1:2048) and rubella-specific IgM. The patient decided to terminate the pregnancy without a repeat blood examination. The correlation coefficient between rubella-specific IgM and rubella HI antibody titers was 0.2527 (p < 0.0001), indicating a weak positive correlation.
Conclusion
This study showed that almost all asymptomatic rubella-specific IgM-positive patients measured in the context of high rubella HI antibody titers were not acutely infected regardless of whether the rubella-specific IgM antibody titer was high or low.
Manual assembly and Human-Robot Collaboration in repetitive assembly processes: a structured comparison based on human-centered performances
Authors: Gervasi Riccardo , Capponi Matteo , Mastrogiacomo Luca , Franceschini Fiorenzo 
Published: December 2022

Abstract
Human-Robot Collaboration (HRC) represents an innovative solution able to enhance quality and adaptability of production processes. In a collaborative process, man and robot cooperate symbiotically. However, to fully exploit the benefits of HRC, human factors must be taken into account. A novel experimental setting involving a repetitive assembly process is presented to investigate the effects of prolonged HRC on user experience and performance. Each participant was involved in two 4-hours shifts: a manual assembly setting and a HRC assembly setting. The response variables collected in the study include the perceived workload, self-reported affective state, perceived body discomfort, physiological indicators of stress (heart rate variability and electrodermal activity), and process defectiveness. The experimental results showed reduced mental effort and fewer process defects in the HRC setting, highlighting how collaborative robotics can also support humans from a cognitive point of view.
Chronically ill Patients’ Perspectives on Support Services and Activities of Patient Organizations
Authors: Zigdon Avi , Eckhaus Eyal , Zigdon Ofek , Rosenfeld Michal 
Published: December 2022

AbstractBackground:Evidence suggests that Patient Organizations (POs) are an important support factor in chronically ill patients' coping with their illness. This support differs significantly in topic, type, and style from the physician support, and they complement each other. Also, the chronically ill Patients' perspective on the activities and services offered by patient organizations has yet to be evaluated. This study aimed to identify and map the services and activities of all types of non-profit POs from the general chronically ill patient's perspective.Methods:Nineteen services and activities of POs were sampled from the activities of patient organizations in Israel and from scientific literature and evaluated by chronically ill patients in Israel. Questionnaires were distributed among patients with chronic diseases (N=1395) using snowball sampling.Results: Exploratory factor analysis (EFA) was performed, followed by confirmatory factor analysis (CFA) for convergent and discriminant validity. Findings showed that twelve services and activities suggested by patient organizations were found to represent chronically ill patients' needs and categorized into three groups: Interpersonal support (five items), patients' rights (three items), and medical information (four items).CFA showed a good fit for the observed data. CFI = 0.98, NFI = 0.97, TLI = 0.96, RMSEA = 0.058.Conclusions:Patient organizations complement services and activities that are not available in health systems. However, patient organizations need to tailor their services and activities to the needs of chronically ill patients, to whom they provide the services, in a beneficial manner that will allow them to maximize their ability to better manage their disease.
An Overview of the Research Trends on Fiber-Reinforced Shotcrete for Construction Applications
Authors: Amin Muhammad Nasir , Al-Samman Ahmad Yahia , Ahmad Waqas , Khan Kaffayatullah , Al-Hashem Mohammed Najeeb , Sahar Umbreen-us , Mohamed Abdullah 
Published: December 2022

Abstract
Research on fiber-reinforced shotcrete (FRS) for rock supports in tunneling and mining is increasing. This study used a novel approach based on scientometric analysis of FRS's bibliographic data to determine its essential components. The potential of manual review articles to link together previously isolated areas of the literature is limited. Knowledge mapping, co-citations, and co-occurrence analysis are the most challenging parts of current research. The information needed to complete the study was retrieved from the Scopus search engine. Important publishing sources, keyword analysis, writers that have the most contribution in terms of citations and publications, the most-cited articles, and the regions most actively engaged in FRS research were identified throughout the data review process. Moreover, the need for FRS and the major constraints associated with their usage were discussed. In addition to its applications in tunneling and mining, FRS can also be used for refractory linings, soil stabilization, structural restorations, and the construction of river barriers, swimming pools, shell structures, domes, and even buildings. The key barriers to employing FRS are the corrosion of fibers, high expense, and uniform fiber dispersion. Using recycled fibers, particularly from discarded tires, coating fibers with zinc/brass/copper, and employing the layer procedure, on the other hand, are superior approaches to addressing these issues. Moreover, with increased concerns about the sustainability of construction materials, efforts need to be carried out for the creation of sustainable FRS by using waste materials. The scientific description and quantitative assessment of regions and scientists might help academics build collaborative projects and disseminate new ideas and methods.
Milky strawberry-like cerebrospinal fluid secondary to hypertriglyceridemia a rare case report of hemorrhagic stroke
Authors: Elzain Mohammed Awad , Osman Mostafa , Othman Mohammed N. Al , Boukhari Rabab Abdulkarim T , Moussa Zaghloul Mahmoud , Alshahrani Saad Safar 
Published: December 2022

Abstract
INTRODUCTION: Disorders of lipid metabolism can lead to intracranial hemorrhages as a rare complication of the disease. To our knowledge only 4 case reports were found to have milky pinkish cerebrospinal fluid secondary to severe hypertriglyceridemia.
CASE DESCRIPTION: Forty years old Indian male, presented to the emergency department with severe headache, vomiting and decreased level of consciousness. His CT Brain showed intraventricular hemorrhage causing secondary hydrocephalus. His lab investigations revealed very high lipid profile specially triglycerides level (70.19 mmol/L). An external ventricular drain (EVD) was inserted and CSF was milky strawberry-like. CSF analysis revealed high lipid profile as well. Both CTA and DSA were unremarkable for any vascular malformations. The patient was successfully managed through a combined effort of neurosurgery, medical and ICU teams. His serum lipid profile came back to normal, his EVD was removed and a ventriculo-perotineal shunt was inserted.
CONCLUSION: The case illustrates the significant high risk for intracranial hemorrhages secondary to hyperlipidemia. Therefore, it may be suggested to obtain a CSF sample via lumbar puncture for example to measure the corresponding CSF lipid profile in patients with extremely high serum levels. This may possibly predict the associated high risk for these devastating intracranial hemorrhages. The guidelines for the serum level at which this has to be performed should be tailored based on multiple randomized multicenter clinical trials. The combined effort of the medical, neurosurgical and ICU teams is the cornerstone in the management of these special cases.
The coenzyme A precursor pantethine restrains sarcoma growth through promotion of type 1 immunity
Authors: Miallot Richard , Millet Virginie , ROGER Anais , Fenouil Romain , Tardivel Catherine , Martin Jean Charles , Shintu Laetitia , Berchard Paul , Lanza Juliane Souza , Malissen Bernard , Ugolini Sophie , Dutour Aurelie , Henri Sandrine , Finetti Pascal , Bertucci francois , Blay Jean-Yves , Galland Franck , Naquet Philippe 
Published: December 2022

Abstract
The tumor microenvironment is a dynamic network of stromal, cancer and immune cells that interact and compete for resources. Mitochondria play an essential role in the control of metabolic plasticity and contribute to tumor progression and immune cell functionality. We previously identified the Vanin1 pathway as a tumor suppressor of sarcoma development via vitamin B5 and coenzyme A regeneration. Using an aggressive sarcoma cell line that lacks Vnn1 expression, we showed that administration of pantethine, a vitamin B5 precursor, impairs tumor growth in immunocompetent mice. Pantethine boosts anti-tumor type 1 immunity including polarization of myeloid and dendritic cells towards enhanced IFNγ-driven antigen presentation pathways and improved development of hypermetabolic effector CD8+ T cells endowed with potential anti-tumor activity. At later stages of treatment, the effect of pantethine is limited by the development of immune cell exhaustion. Nevertheless, its activity is comparable to that of anti-PD1 treatment in sensitive tumors. In humans, VNN1 expression correlates with improved survival and immune cell infiltration in soft tissue sarcomas but not osteosarcomas. Pantethine could be a potential therapeutic immunoadjuvant for the development of anti-tumor immunity.
Combined Gestational Age and Serum Fucose for Early Prediction of Risk for Bronchopulmonary Dysplasia in Premature Infants
Authors: Li Liangliang , Xu Shimin , Yin Xiangyun , Xi Hongmin , Yang Ping , Ma Lili , Zhang Lijuan , Li Xianghong 
Published: December 2022

Abstract
Objective:
Bronchopulmonary dysplasia (BPD) is the most complication of premature infants, accurate identification of at-risk infants and early intervention are key to improve outcomes. The objective of this study is to explore a multifactorial model to predict the risk of BPD.
Materials and methods:
A total of 148 neonates with gestational age ≤ 32 weeks were recruited. Clinical data and serum samples at 1week after birth were collected. Content of serum free monosaccharides and degraded monosaccharides were analyzed by high-performance liquid chromatography (HPLC). The logistic regression analysis was drawn to build models for the early diagnosis of BPD. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic value of different monosaccharides for BPD, and combined with clinical data to improve the diagnostic efficiency, which was calculated using the area under the curve (AUC).
Results:
Of the 148 eligible infants, 42 developed BPD (28.38%), which consist of 71.43% mild (30/42) and 28.57%moderate-severe (12/42) cases. The significant risk factors for BPD in the multivariate analysis of clinical data and serum monosaccharides were gestational age, birth-weight, the duration of invasive mechanical ventilation and non-invasive ventilation, patient ductus arteriosus (PDA) and pregnancy-induced hypertension, glucose (Glc-F), mannose (Man-F), fucose (Fuc-D), galactosamine (GalN-D), galactose (Gal-D), glucose (Glc-D) and mannose (Man-D). In addition, the concentration of Glc-D and Fuc-D inmoderate-severe BPD group were significantly lower than those in mild BPD group. The combination of gestational age and Fuc-D determined the risk of developing BPD, with an AUC value of 0.9964.
Conclusion:
The prediction model based on the gestational age and Fuc-D predicts the development of BPD with high sensitivity (95.24%) and specificity (93.24%) and might facilitate early intervention and improve outcome.
Does Switching Colchicine Preparations Have a Role in The Treatment of Familial Mediterranean Fever?
Authors: Öner Nimet , Çelikel Elif , Tekin Zahide Ekici , Güngörer Vildan , Kurt Tuba , Sezer Müge , Tekgöz Pakize Nilüfer , Karagöl Cüneyt , Coşkun Serkan , Kaplan Melike Mehveş , Polat Merve Cansu , Acar Banu Çelikel 
Published: December 2022

Abstract
Objective: The aim of this study was to evaluate the treatment response of compressed colchicine tablets in familial Mediterranean fever (FMF) patients with resistance or intolerance to coated colchicine. Another objective was to determine the demographic and clinical characteristics of the group that responded to compressed colchicine preparations.
Methods: In this retrospective study, the files of 1574 patients with FMF in our center were reviewed. Sixty-one patients who did not respond to coated colchicine and were switched to compressed colchicine treatment were evaluated. The number of attacks and the International Severity Score for FMF (ISSF) during the 6 months before and 3, 6, 9, 12, and 24 months after switching from coated colchicine to compressed colchicine were recorded.
Results: Of the 61 patients, 36 (59%) were boys, and 25 (41%) were girls. The mean age of symptom onset was 34±22 months. The mean age at diagnosis was 8.4±5.1 years. The mean age at the time of switching from coated colchicine to compressed colchicine was 11.8±3.5 years. Among 61 patients whose treatments were switched to compressed colchicine, 12 had intolerance, while 49 had uncontrolled attacks and persistent subclinical inflammation. The problems of 12 patients who were switched to compressed colchicine due to intolerance were resolved. Response to compressed colchicine was observed in 25/49 patients with uncontrolled attacks and persistent subclinical inflammation. The frequency of attacks decreased significantly at the 3rd and 6th months after switching from coated colchicine to compressed colchicine (p<0.05). The mean ISSF score of the patients in the last 3 months while receiving coated colchicine was 5.4±2.3. The mean ISSF scores of the patients at the 3rd and 6th months after switching to compressed colchicine were 3.2±2.1 and 2.6±1.7, respectively. Twenty-four of the 49 patients did not respond to compressed colchicine. Anakinra was added to the treatments of 14 of the 24 patients, and canakinumab was added to the treatments of 10. The frequency of attacks and ISSF scores of the patients who did not respond to compressed colchicine significantly decreased after the addition of IL-1-targeting drugs to their treatments. At the end of the two-year follow-up, 42 patients were tolerant to compressed colchicine, and 19 patients (4 anakinra, 15 canakinumab) were on compressed colchicine plus IL-1-targeting drugs.
Conclusions: Compressed colchicine was shown to be a useful treatment option before initiating biological agents in patients who were unresponsive to coated colchicine, especially those with side effects.
Morphologic analysis of the foveal avascular zone for prediction of postoperative visual function in advanced idiopathic epiretinal membrane
Authors: Kim Gee-Hyun , Hwang Bo-Een , Chun Heejeong , Kim Joo Young , Kim Rae Young , Kim Mirinae , Park Young-Geun , Park Young-Hoon 
Published: December 2022

Abstract
To investigate the preoperative morphology of the foveal avascular zone (FAZ) for prediction of the postoperative visual function in advanced idiopathic epiretinal membrane (ERM).
21 patients (21 eyes) with unilateral idiopathic ERM who underwent pars plana vitrectomy (PPV) with internal limiting membrane (ILM) peeling were included. Superficial FAZ was measured preoperatively in both eyes using optical coherence tomography angiography (OCTA). Area, perimeter, and circularity of FAZ were achieved, and their rationes between the ERM eyes and the contralateral eyes were calculated to analyze the degree of FAZ distortion in diseased eyes. The best-corrected visual acuity (BCVA) and central foveal thickness (CFT) were measured at baseline and more than 6 months after surgery. The correlations of the preoperative FAZ rationes with BCVA and CFT were assessed.
The FAZ in the eyes with ERM was significantly reduced, and the BCVA was significantly correlated with the area ratio (P = 0.004) and the perimeter ratio (P = 0.005) of the FAZ before surgery. Decimal BCVA changed from 0.326 ± 0.139 to 0.450 ± 0.202 (P = 0.058), and CFT reduced significantly (P = 0.001) after surgery. The preoperative FAZ area and perimeter rationes were significantly correlated with letter score gain (P = 0.001, P < 0.001), and the latter showed significant correlations with the postoperative BCVA (P = 0.031) and CFT reduction ratio (P = 0.008).
The preoperative FAZ perimeter ratio is the best predictor of functional and morphological prognosis after surgery in advanced idiopathic ERM.
Platelet-lymphocyte ratio predicts chemotherapy response and prognosis in patients with gastric cancer undergoing radical resection
Authors: Zeng Qingnuo , wang danfang , wang shilong , bai zilong , nie yuanhua , xu longwen , chang dongmin , zhang xin 
Published: December 2022

Abstract
Introduction

Amounting literatures have reported the significance of systemic inflammatory markers for evaluating tumor prognosis. But few studies have systematically compared their superiority and their impact on adjuvant chemotherapy.

Materials and Methods

We retrospectively enrolled 730 GC patients who underwent radical gastrectomy. Fibrinogen (FIB), platelet-lymphocyte ratio (PLR), systemic inflammation response index (SIRI), prognostic nutritional index (PNI), systemic immune-inflammation index (SII), neutrophil-lymphocyte ratio (NLR) and lymph node ratio (LNR) were grouped according to cutoff values. Their clinical significance for GC prognosis was determined by multivariate COX regression analysis in both overall and subgroups by PLR status. Cases were divided into four groups according to PLR status and adjuvant chemotherapy status and survival was compared between groups.

Results

Multivariate analysis showed that PLR was an independent prognostic factor for overall survival (OS) and disease-free survival (DFS) of GC patients. Adjuvant chemotherapy improved survival more significantly in patients with low PLR than that with high PLR. Among patients receiving adjuvant chemotherapy, low PLR was significantly associated with prolonged survival in TNM stage II, but not in TNM stage III.

Conclusion

Preoperative high PLR is an independent risk factor for GC patients undergoing radical gastrectomy and adversely affects the postoperative chemotherapy effect.