bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of tool flank wear effect on system stability prediction in milling of Ti-6AI-4V thin-walled workpiece
Authors: Ma Junjin , Li Yunfei , Zhang Dinghua , Zhao Bo , Yan Xinhong , Pang Xiaoyan 
Published: May 2022

Abstract
Excessive tool wear can shorten tool life and cause low machining surface quality and efficiency in milling of Ti-6AI-4V thin-walled workpieces. To overcome the effect of time-varying tool flank wear on system stability prediction ignored by conventional method, a system stability prediction model considering tool flank wear effect in milling of Ti-6AI-4V thin-walled workpiece is proposed. In this work, the tool flank wear region is discretized into differential elements, then, the friction effect and process damping effect caused by extruding function between tool and workpiece are analyzed, and a time-varying milling force model is established. In this process, the relationship of cutting tool flank wear band width VB and section radius difference NB is determined, and the indentation volume between tool and workpiece is iteratively calculated, which is used to investigate process damping. After, time-varying milling force coefficients are derived considering different tool flank wear status. Then, in modal space, the evolutionary process of stability lobe diagrams considering tool flank wear effect is determined. Subsequently, to effectively predict system stability, tool flank wear curves and dynamic cutting force coefficients are calibrated by slot milling, and cutting force prediction values considering tool flank wear effect and experimental values in feed direction and normal direction are 7.3% and 12.1%, respectively. Finally, a series of machining tests are conducted to verify the effectiveness of tool flank wear on machining stability in milling of Ti-6AI-4V thin-walled workpieces to some extent, and the experimental results show that the system stability prediction accuracy of the proposed method is improved by 23.8% compared with that using conventional method.
A Prediction Model for Worsening Diabetic Retinopathy after Panretinal Photocoagulation
Authors: Li Jinglan , Li Xuanlong , Lei Mingxing , Li Wanyue , Chen Wenqian , Ma Tianju , Gao Yi , Ye Zi , Li Zhaohui 
Published: May 2022

Abstract
Background
Diabetic retinopathy (DR) as one of the severe complications of diabetes mellitus is the leading cause of blindness in the working age worldwide. While DR still has a high risk of progression after panretinal photocoagulation (PRP) treatment. Hence, this study aims to assess the risk factors and established a model for predicting worsening diabetic retinopathy (DR-worsening) within 5 years after PRP.
Methods
Patients who were diagnosed with severe non-proliferative diabetic retinopathy or proliferative diabetic retinopathy and treated with PRP were included and randomly assigned to either a training or validation cohort. The multivariate logistic regression analysis was used to screen potential risk factors in the training cohort. Then the model was developed and validated using discrimination and calibration.
Results
A total of 271 patients were included, and 56.46% of patients had an outcome of DR-worsening. In the training cohort (n = 135), age (odds ratio (OR) = 0.91, 95% confidence interval (CI) 0.85–0.96), baseline best corrected visual acuity (OR = 0.11, 95% CI 0.01–0.87), diabetic nephropathy (OR = 16.83, 95% CI 1.92–147.50) and hyperlipidemia (OR = 5.53, 95% CI 1.25–24.57) were screened out as the independent risk factors, which were incorporated into the predictive model. The area under the receiver operating characteristic curve and the calibration slope in the training and validation cohort were 0.78, 0.99 (95% CI 0.62–1.35), and 0.79, 1.01 (95% CI 0.54–1.49), respectively. Then, two risk groups were developed, and the actual probability in the low-risk and high-risk groups were 37.42% and 85.19%, respectively.
Conclusions
We developed a model to predict the risk of DR-worsening after PRP treatment within 5 years, which can be used as a rapid risk assessment system and remind us to pay attention to the associated risk factors.
Regulating SQSTM1/P62 and Wnt/β-catenin signaling of EMT to constrain TNBC invasiveness.
Authors: Shome Rajib , Sen Plaboni , Ghosh Siddhartha Sankar 
Published: May 2022

Abstract
Purpose:
The prevalence of cells with mesenchymal features is one of the well-known characteristics of triple negative breast cancers (TNBCs); where cells have undergone epithelial to mesenchymal transition (EMT) and are found to be poorly differentiated, giving rise to cancer stem cells (CSCs). Wnt/β-catenin and Wnt/PCP signaling pathways are prominent contributor of EMT, stemness and CSC properties of TNBC. SQSTM1/P62 cooperates with the components of the Wnt/PCP signaling pathway and is critically involved at the interface of autophagy and EMT. Understanding these regulatory links proves to be an instrumental approach to prohibit progression of TNBC.
Methods:
siRNA targeting SQSTM1/P62 and inhibitor of Wnt/β-catenin (FH535) in conjunction was used to explore molecular modification of EMT and stemness markers. Flow cytometric assays reveled CSC and apoptotic population whereas migration and invasion assay assetred alteration of the metastatic property. Genes and proteins involved were studied at molecular level employing confocal microscopy, western blotting and qRT-PCR.
Results:
Although SQSTM1/P62 is not crucial for cell survival, cytotoxicity assay revealed synergistic interaction between the siRNA/inhibitor. Modulation of these important pathways helped in reduction of expression of genes and proteins contributing to CSC properties. Co-treatment reduced the colony formation and self-renewal properties. Furthermore, invasion assay revealed 5.22-fold downregulations of the metastatic property. Gene and protein expression analysis revealed the induction of EMT to MET. Moreover, co-treatment resulted in inactivation of non-canonical Wnt VANGL2-JNK signaling axis and depletion of p-AKT and p-STAT-3.
Conclusions:
The synergistic impact of inhibition of SQSTM1/P62 and Wnt/β-catenin signaling facilitates the development of a potential theraputic regimen for TNBC.
Prediction of the structural interface between fibroblast growth factor23 and Burosumab using alanine scanning and molecular docking
Authors: Kanhasut Karnpob , Tharakaraman Kannan , Ruchirawat Mathuros , Satayavivad Jutamaad , Fuangthong Mayuree , Sasisekharan Ram 
Published: May 2022

Abstract
Burosumab, an FGF23 targeting monoclonal antibody, was approved by the FDA in 2018 for use in children and adults with X-linked hypophosphatemia (or XLH). While several clinical studies have demonstrated the long-term safety and efficacy of Burosumab, the molecular basis of FGF23-Burosumab interaction which underpins its mechanism of action remains unknown. In this study, we first employed molecular docking combined with alanine scanning of epitope and paratope to predict a model of FGF23-Burosumab interaction. Then, we used the model to understand the species-species cross-reactivity of Burosumab and to reverse engineer mouse FGF23 with 'back to human' mutations to bind Burosumab. Finally, we redesigned the CDRs with two mutations to engineer an affinity enhanced variant of the antibody. Our study provides insights into the FGF23-Burosumab interaction and demonstrates that alanine-scanning coupled with molecular docking can be used to optimize antibody candidates (e.g., structure-guided affinity maturation) for therapeutic use.
Nano pyrite root treatment in conjunction with soil application of goat droppings boost onion yield and anthocyanin and flavonoids content: A nano-organic farming model towards sustainability
Authors: Jangir Himanshi , Kaler Brijesh , Srivast Gaurav , Das Mainak 
Published: May 2022

Abstract
Agrochemical-intensive farming uses synthetic fertilizers, insecticides, pesticides, and weedicides. It holds the most prominent place in modern agriculture This reliance on synthetic agrochemicals has resulted in the declining health of the environment and living species. To restore the ecological balance, we need to explore strategies to minimize agrochemicals’ use without compromising agricultural production. Nano-formulations help lower dosages of agrochemicals, leading to the emergence of nano-agriculture. Yet the critical challenge is how we could exploit the power of nanomaterials to selectively improve the metabolic performance of crop plants. Here we have achieved the same by root treatment of the onion crop and then growing them in the presence of organic goat manure. In two field trials in consecutive years, we report an increase in yield through root treatment with nano-pyrite (FeS2) plus soil application of goat dropping (Test) as compared to the use of goat dropping alone (Control). The total bio-mass (bulb + leaf) weight of the test sample was 4.75 kg (n = 86), while control samples weighed 3.5 kg (n = 86). The total bulb weight for the control and test was 2.6 kg and 3.6 kg, respectively. We observed a yield-boosting effect of root treatment with nano-pyrite in onion crops. There is a significant increase in the anthocyanin content in test bulbs and flavonol content in test leaves. We have integrated two types of farming systems: organic farming and nano-agriculture, resulting in a hybrid form; nano-organic farming to bolster the metabolic fitness of the onion (Allium cepa) to achieve sustainable food production.
Understanding Variation in Oleic Acid Content of High-Oleic Virginia-type Peanut
Authors: Andres Ryan , Dunne Jeffrey Colin 
Published: May 2022

Abstract
The high oleic trait in peanut is conditioned by loss-of-function mutations in a pair of homeologous enzymes and is well known to improve the shelf life of peanut products. As such, the trait is given high priority in current and future cultivars by the North Carolina State University peanut breeding program. For unknown reasons, high oleic cultivars and breeding lines intermittently failed to meet self-imposed thresholds for oleic acid content in internal testing. To determine why, a manual seed chipper, crude DNA isolation protocol, genotyping assays for both mutations, and a web-based SNP calling application were developed. The primary cause was determined to be contamination with normal oleic seeds resulting from inadequate screening protocols. In order to correct the problem, a faster screening method was acquired to accommodate a higher oleic acid threshold. Additionally, results showed the mutation in one homeolog is fixed in the program, dig date has no significant effect on oleic acid content, and minor modifiers segregating within the program explain 6% of the variation in oleic acid content.
Susceptibility of the Anopheles coustani complex to Plasmodium vivax and Plasmodium falciparum infection in Ethiopia
Authors: Tsegaye Arega , Demissew Assalif , Hawaria Dawit , Getachew Hallelujah , Habtamu Kassahun , Asale Abebe , Yan Guiyun , Yewhalaw Delenasaw 
Published: May 2022

Abstract
Background
Insecticide-based vector control interventions along with case management with artemisinin-based combination therapy (ACT) reduced malaria incidence and prevalence worldwide. The current control methods focus on the primary malaria vector, Anopheles gambiae s.l., however, the role of secondary and suspected vectors has been either sidelined or limited. Determining the susceptibility of secondary and suspected vector species to different parasites in time and space is important in malaria control and elimination programs. Thus, this study aimed at assessing the susceptibility of Anopheles gambiea s. l., An. coustani complex and An. pharoensis to Plasmodium vivax and P. falciparum infection in Ethiopia.
Methods
Larvae of Anopheles were collected from different breeding sites and reared to adult under controlled conditions. The adult female mosquitoes were identified to species as An. gambiea s.l, An. coustani complex and An. pharoensis. The three species were allowed to feed in parallel on the same infected blood with gametocytes drawn from P.vivax and P. falciparum gametocemic patients by Indirect Membrane Feeding Assays (IDMFA). Fed mosquitoes were kept under controlled laboratory conditions. After seven days, all survived mosquitoes were dissected to detect mid-gut oocyst using microscopy and enumeration. Data were analyzed using R statistical software package version 4.2.0.
Results
Out of a total 5,915 female Anopheles mosquitoes exposed to gametocyte infected blood, 2,106 (35.60%) mosquitoes fed successfully in the 32 independent infection experiments. There was a significant variation in feeding rates among An. gambiae s l, An. pharoensis and An. coustani complex (G = 48.43, p < 0.0001). All the three exposed Anopheles species were susceptible to P. vivax and P. falciparum infection. The proportion of infected mosquitoes following engorgement on an infected blood meal was significantly different among species (G = 6.49, p < 0.05). The median intensity of infection for An. coustani complex, An. gambiae s.l. and An. pharoensis was 1.16, 2.00 and 1.25, respectively. Even though, the proportion of infected mosquitoes significantly differed in intensity of infection, infection rate and mean oocysts among the species, gametocyte density was highly correlated to infection rate and mean oocyst of infection all tests (p < 0.001).
Conclusion
Anopheles coustani complex were susceptible to both P. vivax and P. falciparum infection. An effective malaria elimination program might include tools that target outdoor vector surveillance and control, which reduce focal malaria transmission.
Transverse Oscillations of Coronal Loops induced by eruptions of Magnetic Flux Tube and Plasmoid
Authors: K. Safna Banu , Maurya R. A. , P.T. Jain Jacob 
Published: May 2022

Abstract
We studied the transverse oscillations in hot coronal loops of an active region NOAA 12673 located at the west limb. Loop oscillations were associated with a plasmoid ejection from the same area. During the rising phase of the plasmoid, a magnetic flux tube was seen to be rising and bending towards the loop system that erupted before the plasmoid ejection. In addition to the plasmoid ejection, a large coronal mass ejection (CME) and a X8.2 flare was observed in the same active region for several hours (~7 hours). A follow-up shock wave from the flare site after the plasmoid ejection was possibly triggered by sudden momentum transfer towards the solar disc. It was found to be propagating across the entire solar disc with an average speed of ~1290 km s-1. The combined effects of the erupting flux tube, plasmoid ejection and the shock wave perturbed the loops from their equilibrium and set them in oscillations. We found different oscillations of fundamental mode in loops, fast decaying (with a period of 7.20 minutes and a damping time of ~16.00 minutes) and slow decaying (with a period of ~5.75 minutes and a damping time of ~30.78 minutes). The two different oscillations could be due to different loop lengths and plasma densities. These loops were found to be oscillating with a phase difference of ~33 degrees. This could be due to varying times of their excitation, possibly caused by the flux tube's eruption and the plasmoid ejection.
Antioxidant and Antimicrobial activity of Lycoperdon pyriforme fruiting body
Authors: Asfaw Melese Damtew 
Published: May 2022

Abstract
Lycoperdon pyriforme used in is traditional medicine by locals in different parts of Ethiopia. The study aimed to evaluate the antioxidant, antimicrobial and phytochemical constituents of the methanol extract of Lycoperdon pyriforme which may infer the antioxidant and antimicrobial activity of this fungi. DPPH radical scavenging, total phenol content assay and total flavonoid content assay were done to determine the antioxidant activity of the fungi extract. Agar disc diffusion method was used to determine the antimicrobial properties against S. aureus, B. subtilis, C. albicans, E. coli in a dose-dependent manner. The fungi extract was found to show significant antimicrobial and antioxidant properties. The presence of phenol and flavonoid compounds present in the fungi could be the reason behind the antioxidant and antimicrobial activity. The current study shows that Lycoperdon pyriforme could be a good source for preparation of antibacterial, antifungal and antioxidant extracts in future.
Neuroprotective and anti-inflammatory effects of Yingqiao San on epileptogenesis are mediated by inhibiting HMGB1 translocation and regulating the HMGB1/TLR4 signaling pathway
Authors: Chen Hanjiang , Zhang Xilian , Song Ying , Chen Zhaoyuan , Lu Yanli , Rong Ping , Ma Rong 
Published: May 2022

Abstract
Background: Epileptogenesis was the latency period of epilepsy. Neuronal damage and subsequent inflammatory response were the basic mechanisms of epileptogenesis. From now on, there was still no effective drugs to prevent the process of epileptogenesis. Yingqiao San, a famous classic prescription over 400 years in China, was widely used in sorts of infectious disease. In preclinical study of childhood epilepsy complicated with upper respiratory tract infections, we found that YQS can not only reduce the frequency of respiratory tract infections, but also decrease the frequency and duration time of seizures, and can alleviate sterile inflammation to some extent. HMGB1, an important late mediator in sterile inflammation, has been seem as a potential target of epileptogenesis. So we hypothesized that YQS had effect of antiepileptogenic, may be related to inhibit the translocation of HMGB1 and modulate neuroinflammation.Methods: The pilocarpine-induced status epileptic model was adopted to induce brain injury model in mice. Observation and sampling were performed at 7 days, 14 days and 28 days. The number of seizures, duration time of seizures, mortality rate and the appearance of brain were employed to determine the anti-seizure effects of YQS. The Nissl staining, FJB staining were employed to determine the neuroprotective of YQS. The double immunofluorescent staining were employed to observe inflammatory response and the location of HMGB1 in glia cells. The Western blot and immnuohistochemical staining were used to calculate the quantitative and qualitative of HMGB1 to detect the translocation of HMGB1 and the expression of HMGB1/TLR4 signaling pathway. Results: It was found that YQS can effectively decrease the frequency of seizures, shorten duration time of seizures, reduce the mortality of mice and alleviate brain edema. YQS can significantly attenuate neuronal damage and inhibit the activation and proliferation of microglia and astrocytes with time-dependent. Moreover, YQS can modulate the subsequent inflammatory response by inhibiting HMGB1 translocation and regulating the expression of HMGB1/TLR4 signaling pathway.Conclusions: YQS can prevent the process of epileptogensis. Its neuroprotective and anti-inflammatory effects on epileptogenesis may mediate by inhibiting HMGB1 translocation and modulating neuroinflammation. It can still be used as an adjunctive therapy for treatment of epilepsy.
Is sentinel lymph node biopsy without frozen section in early stage breast cancer sufficient in accordance with ACOSOG-Z0011? A retrospective review from King Chulalongkorn Memorial Hospital.
Authors: Treeratanapun Nattanan , Lerttiendamrong Bhoowit , Vacharathit Voranaddha , Tantiphlachiva Kasaya , Vongwattanakit Phuphat , Manasnayakorn Sopark , Vongsaisuwon Mawin 
Published: May 2022

Abstract
Background: In 2021, increased implementation of the usage of permanent section (PS) without intraoperative frozen section (FS) for sentinel lymph node biopsy (SLNB) were evident worldwide. This pilot study conducted in Thailand will determine the re-operation rate in SLNB without FS technique. Method: we retrospectively reviewed 239 cases of SLNB without FS in King Chulalongkorn Memorial Hospital from April 2016 to April 2021. These patients were diagnosed with primary invasive breast cancer with clinical node negative. The clinical nodal status was assessed from physical examination. The re-operation rate was evaluated by the number of positive SLNs; equal or more than 3 nodal metastases were subjected to second surgical procedure. Result: Between April 2016 and April 2021, there are 239 patient who had undergone SLNB in accordance with ACOSOG Z0011 criteria with the use of PS alone. A total of 975 SLNs were removed from these 239 patients, with an average of 4.15 nodes per patient. Out of 239 patients, 21 (8.8%) and 6 (2.5%) had metastatic disease in 1 and 2 nodes, respectively. The remaining 212 (88.7%) patients had no nodal metastasis. None of the patients were subjected to a second surgical procedure.Conclusion: With a re-operation rate of 0%, we concluded that the implementation of SLNB with PS analysis alone in patients who satisfied the ACOSOG Z0011 criteria do not altered the standard of care offered by additional FS analysis. With cessation of intraoperative procedure, operation cost, operation time and anesthetic side effect are projected to decrease.
Preference Heterogeneity in Health Valuation: A Latent Class Analysis of the Peru EQ-5D-5L Values
Authors: Karim Suzana , Craig Benjamin M , Tejada Romina Arely , Augustovski Federico 
Published: May 2022

Abstract
Background
Preference heterogeneity in health valuation has become a topic of greater discussion among health technology assessment agencies. To better understand heterogeneity within a national population, valuation studies may identify latent groups that place different absolute and relative importance (i.e., scale and taste parameters) on the attributes of health profiles.
Objective
Using discrete choice responses from a Peruvian valuation study, we estimated EQ-5D-5L values on a quality-adjusted life-year (QALY) scale accounting for latent heterogeneity in scale and taste, as well as controlling heteroskedasticity at task level variation.
Method
We conducted a series of latent class analyses, each including the 20 main effects of the EQ-5D-5L and a power function that relaxes the constant proportionality assumption (i.e., discounting). Taste class membership was conditional on respondent-specific characteristics as well as their experience with the time trade-off tasks. Scale class membership was conditional on behavioral characteristics such as survey duration and self-stated difficulty level in understanding tasks. Each analysis allowed the scale factor to vary by task type and time (i.e., heteroskedasticity).
Results
The results indicate three taste classes: a quality-of-life oriented class (33.35%) that placed the highest value on levels of severity, a length-of-life oriented class (26.72%) that placed the highest value on life span, and a middle class (39.71%) with health attribute effects lower than the quality class and life span effect lower than the length-of-life oriented class. The EQ-5D-5L values ranged from − 2.11 to 0.86 (quality-of-life oriented class), from − 0.38 to 1.02 (middle class), and from 0.36 to 1.01 (length-of-life oriented class). The likelihood of being a member of the quality-of-life class was highly dependent on whether the respondent completed the time-tradeoff tasks (p-value < 0.001). The results also show two scale classes as well as heteroskedasticity within each scale class.
Conclusion
Accounting for taste and scale classes simultaneously improves understanding preference heterogeneity in health valuation. Future studies may confirm the differences in taste between classes in terms of the effect of quality of life and life span attributes. Furthermore, confirmatory evidence is needed on how behavioral variables captured within a study protocol may enhance analyses of preference heterogeneity.