bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitrites, Nitrates, and Cardiovascular Outcomes: Are We Living “La Vie en Rose” With Pink Processed Meats?
Authors: Srour Bernard , Chazelas Eloi , Fezeu Léopold K. , Javaux Guillaume , Pierre Fabrice , Huybrechts Inge , Hercberg Serge , Deschasaux‐Tanguy Mélanie , Kesse‐Guyot Emmanuelle , Touvier Mathilde 
Published: December 2022

Background
Nitrates and nitrites are used as food additives in processed meats. They are also commonly ingested from water and several foods. Several short‐term clinical studies suggested beneficial effects of dietary nitrates on blood pressure, while deleterious effects on oxidative damage have been suggested in some experimental studies. However, there is a lack of evidence from longitudinal epidemiological studies linking foods and water‐originated and additives‐originated nitrites and nitrates, separately, to hypertension and cardiovascular diseases risk. We aimed to study these associations in a large population‐based cohort.

Methods and Results

Overall, 106 288 adults from the French NutriNet‐Santé cohort (2009–2022) were included. Associations between nitrites and nitrates intakes and hypertension and cardiovascular disease risks were assessed using multi‐adjusted Cox proportional hazard models. During follow‐up, 3810 incident cases of hypertension and 2075 cases of cardiovascular diseases were ascertained. Participants with higher intakes of additives‐originated nitrites (sodium nitrite in particular [European code e250]) had a higher hypertension risk compared with nonconsumers (hazard ratio, 1.19 [95% CI, 1.08–1.32],
P
=0.001, and 1.19 [95% CI, 1.08–1.32],
P
=0.002), respectively. No association was detected between foods and water‐originated nitrites, or nitrates with hypertension risk (all
P
values >0.3). We found no association between nitrites or nitrates and risks of cardiovascular diseases (all
P
values >0.2).

Conclusions
These results do not support a protective role of nitrites or nitrates in cardiovascular health. Instead, they suggest a positive association between nitrites from food additives and hypertension risk, which needs confirmation in other large‐scale studies. These findings provide new evidence in the context of current discussions about updating regulations on the use of nitrites as food additives.
International Law and the Provocations of the Digital: The 2021 Annual Kirby Lecture in International Law
Authors: Johns Fleur 
Published: December 2022

Abstract
Based in Montevideo, and most recently valued at US$5 billion, the payments platform DLocal enables companies such as Booking.com, Amazon and Uber to transact in local currencies in 29 countries. It specializes in the “emerging economies” of Latin America, the Asia Pacific, the Middle East and North Africa. Among international lawyers, however, Montevideo is best known for another form of international infrastructure. That is the 1933 Montevideo Convention, or at least its first article, standardising the template of modern statehood. At the time, as scholars of international legal history have shown, this amounted to a radical reformatting of the fundamentals of international law, driven by semi-peripheral states, as part of a widespread effort of reconstructive codification after the Great War. Today, DLocal’s Montevideo is emblematic of a very different kind of international legal reformatting now underway. A digital logic, and associated circuits of value and aggregations of power, are becoming embedded—even predominant—in many of international law’s most routine operations. To shed light on this phenomenon, this lecture revisits each component of the Montevideo Convention’s well-known formula for statehood—permanent population; defined territory; government; and the conduct of international relations. Taking efforts of so-called digital humanitarianism as illustrative, it examines how each of these Montevideo properties is being rerouted and recomposed digitally, often in tension with an analog logic characteristic of law. And in the ensuing dislocations sometimes in evidence between analog and digital aspects of international legal work, it identifies some possibilities for collective reworking.
Can Polar Stratospheric Clouds Explain Arctic Amplification?
Authors: Dutta Deepashree , Sherwood Steven C. , Jucker Martin , Gupta Alex Sen , Meissner Katrin J. 
Published: December 2022

Abstract
Climate models underestimate the magnitude of Arctic warming in past warm climates, like the early Cretaceous and Paleogene periods, implying that certain physical processes might be missing or poorly represented. Previous studies suggest that a large increase in wintertime Arctic polar stratospheric clouds (PSCs) might have promoted Arctic amplification through additional greenhouse warming. High methane concentrations in warm climates might have increased stratospheric water vapor providing favorable conditions for PSCs. However, methane concentrations in past warm climates are extremely uncertain. Here, we revisit the PSC hypothesis by exploring PSC changes under very high methane levels, 4× preindustrial carbon dioxide, and strong polar-amplified surface warming, using a whole-atmosphere model with fully interactive chemistry. We find that with polar-amplified warming there is a large increase in Arctic outgoing longwave radiation (OLR) that reduces as the methane concentration is increased. PSCs increase monotonically with methane concentration. A large radiative cooling and an increase in water vapor in the stratosphere increases Arctic PSCs, which follow a power law with respect to relative humidity. Using a two-way partial radiative perturbation technique, we show that the OLR reduction due to PSCs is similar to the direct radiative forcing of methane for high methane levels. Thus, we find that PSCs could play an important role in Arctic warming in a warmer-than-present-day climate, but only if methane levels were higher than suggested by previous modeling studies for past warm climates.
Structural and Environmental Characteristics of Western Baiu Frontal Depressions
Authors: Tochimoto Eigo , Yanase Wataru 
Published: December 2022

Abstract
Baiu frontal depressions (BFDs) are the major cyclones that form and develop along baiu fronts. The structure and environment of BFDs are examined using the Japanese 55-year Reanalysis dataset. BFDs detected automatically were categorized into four groups based on the region where the BFDs peaked: the East China Sea (ES), off the south coast of the Japanese islands (SC), the Yellow Sea (YS), and the Sea of Japan (SJ). A BFD-centered composite analysis demonstrates that, for YS-BFDs and SJ-BFDs, compared to ES-BFDs and SC-BFDs, the strength of upper-level troughs is greater, and the upper-level troughs are closer, suggesting that the effect of upper-level troughs on BFD development is greater. BFDs for all categories are located in the region of cyclonic horizontal shear and have a horizontal trough running from southwest to northeast, favoring barotropic energy conversion. The BFD-centered composite analysis of the energy conversion terms demonstrates that the barotropic energy conversion of mean kinetic energy due to the environmental cyclonic horizontal shear to eddy kinetic energy contributes to BFD development, and the conversion of eddy available potential energy, which is generated mainly by the diabatic heating, is the major contributor to eddy kinetic energy.
Using a condom or glove to improve pelvic exam visualization: A guide
Authors: Palsgaard Peggy , Yerrabelli Rahul S , Hojati Ashkhan , Whitmore Elsa 
Published: December 2022

Objectives: Pelvic exams are a cornerstone of gynecological care, necessary for both regular screenings and diagnostics. The collapse of lateral vaginal walls during a pelvic exam is a frequently encountered problem in clinical practice. Methods: Practitioners often utilize tools found in a typical clinical setting to counter this issue, such as a condom or glove over the speculum to prevent the lateral vaginal walls collapsing inward and obscuring cervical views. Results: These techniques have been passed down from mentor to mentee over the years, though scarcely described in literature. This article aims to provide instructions on how to use these two methods in clinical practice to improve pelvic exams for the practitioner and the patient. Conclusion: Utilizing a condom or glove to prevent lateral vaginal wall collapse has the potential to improve pelvic exams for both practitioners and patients.
Scientists benefit greatly from K-12 partnerships: the Panama Research Experiences for Teachers project
Authors: MacFadden Bruce J. , Vargas Vergara Claudia , Davey Bradford T. 
Published: December 2022

AbstractScientist-teacher partnerships are highly beneficial to K-12 STEM education. While much is known about the benefits for teachers in these partnerships, the corresponding benefits for scientists are less well known. With emphasis on the scientists’ perspective, here we describe our NSF RET (Research Experiences for Teachers) project consisting of five successive cohorts from 2012 to 2016. Coincident with a “once-in-a-century” expansion of the Panama Canal, the science research focused on the paleontology, evolutionary biology, and geology of this region to better understand the ancient Neotropical biota related to the Great American Biotic Interchange (GABI). In the field, scientists and teachers worked together collecting fossils and geological samples. Back in the K-12 classrooms, lesson plans related to their experiences were implemented and the teachers hosted scientist role-model visits. More than 30 scientists and 44 teachers participated in this Panama “GABI RET” project. Using a new validated survey developed during this project and focus groups, we explored the impact of this project, and in particular the perceived benefits accrued by the scientists. Our study confirmed that scientists felt they improved their communication skills, had a better appreciation for the K-12 teaching professions, greatly enjoyed working with the teachers, considered them colleagues, and many wanted to continue K-12 outreach as part of their careers. Overall, scientists perceived that they greatly benefited from these partnerships. In addition to describing their activities, they had numerous recommendations for similar partnerships in the future. For example, these include: (1) having more teachers participate in multiple cohorts, (2) continued opportunities for teachers to be involved in professional meetings, (3) ongoing webinars and face-to-face engagement, and (4) more diversity of racial and ethnic backgrounds, subjects taught, and regions represented. Although this case study was focused on the GABI RET, our results also potentially inform other projects that involve scientists’ education and outreach activities.
Mental healthcare-seeking behavior of women in Bangladesh: content analysis of a social media platform
Authors: Koly Kamrun Nahar , Tasnim Zarin , Ahmed Sharmin , Saba Jobaida , Mahmood Rudbar , Farin Faria Tasnim , Choudhury Sabrina , Ashraf Mir Nabila , Hasan M. Tasdik , Oloniniyi Ibidunni , Modasser Rifath Binta , Reidpath Daniel D. 
Published: December 2022

Abstract
Background
Mental health remains a highly stigmatized area of healthcare, and people often conceal their concerns rather than seek assistance or treatment. The Women Support Initiative Forum (WSIF) is a social media platform established in 2018 to provide expert and peer-led psychosocial support services to women of all ages in Bangladesh. The anonymous nature of the forum means that mental health concerns can be aired without fear of identification.

Method
A content analysis was conducted on the anonymous posts retrieved from the WSIF platform between 8th March 2020 and 7th July 2022. Around 1457 posts were initially selected for analysis which was reduced to 1006 after removing duplicates and non-relevant posts, such as queries about the addresses of the doctors and other non-mental health-related issues. A thematic analysis of the data was conducted using an inductive approach.

Result
The 1006 posts generated four themes and nine sub-themes. All the women mentioned mental health symptoms (n = 1006; 100%). Most also mentioned reasons for seeking mental healthcare (n = 818; 81.31%), healthcare-seeking behavior (n = 667; 66.30%), and barriers to seeking mental healthcare (n = 552; 54.87%). The majority of women described symptoms of stress, depression, and anxiety-like symptoms, which were aggregated under common mental health conditions. Mental health symptoms were ascribed to various external influences, including marital relationship, intrafamilial abuse, and insecurities related to the COVID-19 pandemic. A large proportion of posts were related to women seeking information about mental healthcare services and service providers (psychologists or psychiatrists). The analysis found that most women did not obtain mental healthcare services despite their externalized mental health symptoms. The posts identified clear barriers to women accessing mental health services, including low mental health literacy, the stigma associated with mental healthcare-seeking behavior, and the poor availability of mental health care services.

Conclusion
The study revealed that raising mass awareness and designing culturally acceptable evidence-based interventions with multisectoral collaborations are crucial to ensuring better mental healthcare coverage for women in Bangladesh.
Elective egg freezing patients may benefit from increasing the maximal daily gonadotropin dose above 300IU
Authors: Orvieto Raoul , Aizer Adva , Saar-Ryss Bozhena , Marom-Haham Lilach , Noach-Hirsh Meirav , Haas Jigal , Nahum Ravit 
Published: December 2022

Abstract
Objective
Nowadays, patients attempting social/elective egg freezing has spread globally. Ovarian stimulation (OS) with high daily gonatotropin doses, are commonly offered to this group of patients, aiming to achieve the maximal oocytes cohort with minimum IVF cycle attempts. We aim to assess the IVF-ET outcome, and specifically the oocyte yield, of patients undergoing two successive IVF cycle attempts for elective egg freezing (EEF), and whether changing the daily gonadotropin dose in the second IVF cycle attempt, affect the outcome.

Patients and methods
All women admitted to our IVF unit for social/EEF, who underwent 2 consecutive IVF cycle attempts, with only those who used in the first attempt a starting daily gonadotropin dose of 300 IU were included. Ovarian stimulation characteristics, duration of OS, number of retrieved oocytes, number of mature oocytes were assessed and compared between the 1st and the 2nd IVF cycle attempts, and between the different daily gonadotropin doses and the oocyte yields in the 2nd cycle attempt (increase, decrease or no change).

Main outcome measures
Oocytes and mature oocytes yield in the 2nd as compared to the 1st IVF cycle attempt.

Results
A reduced oocyte yield in the 2nd cycle attempt was observed in those who highly responded in the 1st attempt, regardless the daily dose in the 2nd cycle attempt (whether it was increased, no change and decreased). Moreover, the proportion of patients with same or more oocytes in the 2nd IVF cycle attempt was significantly lower in patients with high peak E2 levels, compared to those with peak E2 levels < 9175 pmol/L. Among patients with high peak E2 (> 9175 pmol/L), those who achieved a lower oocytes yield in the 2nd IVF cycle attempt had lower basal Day-3 FSH/LH ratio (1.5 + 0.5 vs 1.8 + 0.8, p < 0.03) and higher oocyte (range: 7–28, median:10; vs range: 2–15, median:7) and mature oocytes yields. With a cut-off of 9 oocytes, 78.8% of those with > 9 oocytes and 61.8% of those with < 9 oocytes will achieve lower/higher oocytes yield in the 2nd IVF cycle attempt, respectively.

Conclusions
Ovarian stimulation with high daily gonatotropin doses (300 IU) should be offered to patients attempting social/EEF. Moreover, in their 2nd IVF cycle attempt, those with high peak E2 (> 9175 pmol/L) in the 1st attempt, and basal Day-3 FSH/LH ratio < 1.5 and/or more than 9 oocytes retrieved, should receive same OS protocol with no change in the daily gonadotropin dose.
Docetaxel suppressed cell proliferation through Smad3/HIF-1α-mediated glycolysis in prostate cancer cells
Authors: Peng Junming , He Zhijun , Yuan Yeqing , Xie Jing , Zhou Yu , Guo Baochun , Guo Jinan 
Published: December 2022

Abstract
Background
Tumor glycolysis is a critical event for tumor progression. Docetaxel is widely used as a first-line drug for chemotherapy and shown to have a survival advantage. However, the role of docetaxel in tumor glycolysis remained poorly understood.

Methods
The effect of Docetaxel in tumor glycolysis and proliferation were performed by CCK-8, Western blotting, real-time PCR, glucose, and lactate detection and IHC. ChIP and luciferase assay were used to analyze the mechanism of Docetaxel on Smad3-mediated HIF-1α transactivity.

Results
In this study, we showed that docetaxel treatment led to a significant inhibition of cell proliferation in prostate cancer cells through PFKP-mediated glycolysis. Addition of lactate, a production of glycolysis, could reverse the inhibitory effect of docetaxel on cell proliferation. Further analysis has demonstrated that phosphorylation of Smad3 (Ser213) was drastically decreased in response to docetaxel stimulation, leading to reduce Smad3 nuclear translocation. Luciferase and Chromatin immunoprecipitation (ChIP) analysis revealed that docetaxel treatment inhibited the binding of Smad3 to the promoter of the HIF-1α gene, suppressing transcriptional activation of HIF-1α. Moreover, ectopic expression of Smad3 in prostate cancer cells could overcome the decreased HIF-1α expression and its target gene PFKP caused by docetaxel treatment. Most importantly, endogenous Smad3 regulated and interacted with HIF-1α, and this interaction was destroyed in response to docetaxel treatment. What’s more, both HIF-1α and PFKP expression were significantly reduced in prostate cancer received docetaxel treatment in vivo.

Conclusion
These findings extended the essential role of docetaxel and revealed that docetaxel inhibited cell proliferation by targeting Smad3/HIF-1α signaling-mediated tumor Warburg in prostate cancer cells.
Early and midterm outcomes of a bentall operation using an all-biological valved BioConduit™
Authors: Botea Roxana , Lavie-Badie Yoan , Goicea Alexandru , Porterie Jean , Marcheix Bertrand 
Published: December 2022

Abstract
Objectives
To analyze the midterm results of aortic root replacement using the valved, all biological, No React®, BioConduit™.

Methods
From 2017 to 2020, we prospectively followed 91 consecutive patients who underwent a Bentall procedure with a BioConduit™ valved graft in our institution. The primary outcomes were aortic bioprosthetic valve dysfunction and mortality according to Valve Academic Research Consortium 3 (VARC3).

Results
Mean age was 70 ± 10 years and 67 patients (74%) were men. Ascending aortic aneurysm (72%), aortic valve regurgitation (51%) or stenosis (20%) and acute endocarditis (14%) were the main indications for surgery. Seventy-four patients (81.3%) were followed up at 1 year. The perioperative mortality was 8% (n = 8), the early, 1 year, mortality was 2% (n = 2) and the midterm mortality, at 4 years of follow up, was 4% (n = 3). Ten patients fulfilled the criteria for hemodynamic valve deterioration at 1 year (13%) and 14 for a bioprosthetic valve failure during the entire follow-up (17%).

Conclusions
We are reporting early and midterm results of Bentall procedures with the all-biological, valved, No-React® BioConduit™. To our knowledge, this is the first study reporting an early and midterm unexpectedly high rate of non-structural prosthetic hemodynamic deterioration. The rate of endocarditis and atrioventricular disconnections remain similar to previous studies.
Response to commentary ‘long COVID-19/post-COVID condition in children: do we all speak the same language?
Authors: Trapani Gianfranco , Verlato Giuseppe , Bertino Enrico , Maiocco Giulia , Vesentini Roberta , Spadavecchia Alessia , Dessì Angelica , Fanos Vassilios 
Published: December 2022

AbstractHere we present the Authors’ answer to the Letter written by Dr. Garazzino and Colleagues with reference to our article “Long COVID-19 in children: an Italian cohort study”.
Flower-like meristem conditions and spatial constraints shape architecture of floral pseudanthia in Apioideae
Authors: Baczyński Jakub , Celep Ferhat , Spalik Krzysztof , Claßen-Bockhoff Regine 
Published: December 2022

Abstract
Background
Pseudanthia are multiflowered units that resemble single flowers, frequently by association with pseudocorollas formed by enlarged peripheral florets (ray flowers). Such resemblance is not only superficial, because numerous pseudanthia originate from peculiar reproductive meristems with flower-like characteristics, i.e. floral unit meristems (FUMs). Complex FUM-derived pseudanthia with ray flowers are especially common in Apiaceae, but our knowledge about their patterning is limited. In this paper, we aimed to investigate both the genetic and morphological basis of their development.

Results
We analysed umbel morphogenesis with SEM in six species representing four clades of Apiaceae subfamily Apioideae with independently acquired floral pseudanthia. Additionally, using in situ hybridization, we investigated expression patterns of LEAFY (LFY), UNUSUAL FLORAL ORGANS (UFO), and CYCLOIDEA (CYC) during umbel development in carrot (Daucus carota subsp. carota). Here, we show that initial differences in size and shape of umbel meristems influence the position of ray flower formation, whereas an interplay between peripheral promotion and spatial constraints in umbellet meristems take part in the establishment of specific patterns of zygomorphy in ray flowers of Apiaceae. This space-dependent patterning results from flower-like morphogenetic traits of the umbel which are also visible at the molecular level. Transcripts of DcLFY are uniformly distributed in the incipient umbel, umbellet and flower meristems, while DcCYC shows divergent expression in central and peripheral florets.

Conclusions
Our results indicate that umbels develop from determinate reproductive meristems with flower-like characteristics, which supports their recognition as floral units. The great architectural diversity and complexity of pseudanthia in Apiaceae can be explained by the unique conditions of FUMs—an interplay between expression of regulatory genes, specific spatio-temporal ontogenetic constraints and morphogenetic gradients arising during expansion and repetitive fractionation. Alongside Asteraceae, umbellifers constitute an interesting model for investigation of patterning in complex pseudanthia.