bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oscilopatología en Trastornos del Espectro Autista
Authors: Morales Rojas Daniela Alexandra 
Published: January 2022

El lenguaje como vehículo del desarrollo humano incide de importante manera en los procesos sociales, que figuran de forma determinante en el establecimiento de condiciones de vida y bienestar óptimas. Estudios modernos han encontrado que las habilidades que hacen del lenguaje una actividad de compleja integración multisensorial están determinadas por los ritmos de oscilación de las diferentes ondas cerebrales descritas desde las neurociencias. En la literatura se ha descrito un patrón anormal de oscilaciones, responsable de las deficiencias comunicativas en individuos con Trastornos del Espectro Autista y que junto con sus déficits sociales dan como resultado un perfil típicamente característico. Las ondas cerebrales demuestran tener incidencia en la manera en que se regulan los estados mentales que posibilitan tareas de alta demanda cognitiva como el aprendizaje, la comunicación y la comprensión del discurso, dificultades generalizadas en esta población. Los hallazgos aquí presentados, producto de una juiciosa revisión bibliográfica, se caracterizan dentro de la fonoaudiología haciendo posible aterrizar los procesos de intervención a las necesidades lingüísticas y cognitivas específicas de estos individuos, valiéndose de mecanismos biológicos y dando como posible resultado, procedimientos de mayor relevancia y eficacia, que mejoren la calidad de vida de los usuarios y motiven, además, a los profesionales en fonoaudiología y área afines, a ampliar su conocimiento en este interesante campo.
Caracterización Y Tipos De Errores Articulatorios En Pacientes Con Labio Y Paladar Hendido Bogotá( Colombia)
Authors: Jara Moreno Yasmir Brisel , Sarmiento Viasus Olga Isabel 
Published: January 2022

Introducción: La condición de labio y paladar hendido (LPH) es una malformación abundante al nivel mundial; en Colombia la presentan 16 por cada 10000 nacidos vivos, resultando mayormente en Trastorno del habla por presencia de Articulaciones compensatorias.
Objetivo: Caracterizar a la población colombiana residentes en Bogotá respecto a errores articulatorios y fonemas afectados por el antecedente de LPH.
Materiales y métodos: Se incluyeron 108 historias clínicas de pacientes evaluados en el año 2016 tomando datos de valoración fonoaudiológica y adaptación de Universal Parameters for Reporting Speech Outcome in individuals with Cleft Palate.
Resultados: El 75% de la población requiere terapia de habla predominando la presencia de oclusión glotal y distorsión de los fonemas dentoalveolares oclusivo /t/ y fricativo /s/. El 41,66% de ellos presenta fistula e hipernasalidad. El desempeño del sexo femenino es significativamente inferior. Una mayor presencia de errores en los grupos de: PH y rango de edad de 7 a 36 años.
Conclusiones: Los profesionales de fonoaudiología deben centrarse en la intervención temprana de personas con LPH para lograr un adecuado desempeño en deglución, audición, lenguaje y habla para así disminuir secuelas y repercusiones en su calidad de vida.
Fundamentos y Método de las Revisiones Sistemáticas
Authors: Berra Silvina 
Published: January 2022

La práctica profesional requiere el manejo de la evidencia científica actualizada sobre los asuntos en los que a diario se deciden diagnósticos o intervenciones. Las revisiones sistemáticas (RS) son síntesis cualitativas y, a veces, cuantitativas sobre la evidencia disponible respecto de un tema puntual. Este trabajo contextualiza su origen en el movimiento de la medicina basada en la evidencia y las define como un tipo de investigación orientada a buscar, evaluar y sintetizar conocimiento científico previamente producido. A fin de comprender la importancia de la exhaustividad y la rigurosidad del método, se distingue una RS de una revisión narrativa. El método de RS se presenta en una serie de fases o pasos que abarca desde el planteo de una pregunta de investigación, pasando por las estrategias de búsqueda y técnicas e instrumentos para la evaluación de calidad, extracción y análisis de datos, la posibilidad de un metanálisis y, finalmente la interpretación de resultados. Dentro de los criterios de evaluación de la evidencia científica, un pilar fundamental de este tipo de investigación, se explican la validez interna, la precisión y la validez externa de los resultados como componentes de la calidad, y la cantidad y la consistencia de la evidencia científica, a partir de las cuales se pueden establecer las recomendaciones.
Clasificación Del Nivel De Riesgo Por Errores Funcionales de la Deglución (CRAF)
Authors: Vargas Garcia Miguel Antonio , Cely Arboleda Angelica , Suarez Juliza Tatiana , Vélez Gutiérrez Diego Alejandro 
Published: January 2022

La disfagia es conocida como la dificultad o incapacidad a la hora de la ingesta de alimentos, la cual es analizada desde diferentes puntos ya que puede ser generada por factores anatómicos, sean estos de nacimiento o adquiridos, causas psicológicas o funcionales. El quehacer profesional en disfagia permite clasificar la severidad de la disfagia, pero no la calificación del riesgo de los distintos errores funcionales durante la ingesta. A razón de ello, este escrito propone clasificar el nivel de riesgo por errores funcionales de la deglución.
La investigación se planteó desde una naturaleza prospectiva con relación de validez, a través del método Delphi, mediante la cual se postuló una lista de errores funcionales de la deglución para ser validada por 6 jueces expertos en el área. Se realizó una propuesta en una matriz, que les permitió evaluar los distintos errores funcionales divididos en las fases deglutorias y los niveles de riesgo para repercutir en disfagia, según los ítems de suficiencia, relevancia, coherencia y claridad. Se obtuvo una lista de 38 errores validados, los cuales se fundamentan como riesgo, dado que al materializarse fomentan en la secuencia deglutoria una mayor probabilidad de penetración/aspiración de alimento hacia vía aérea. El consenso de la clasificación de estos errores según el riesgo, será una herramienta útil para profesionales del área. Se concluye, a través de la diferencia mínima significativa (LSD) de Fisher para medias, una lista de riesgos por errores funcionales de la deglución, en orden descendente según la severidad del riesgo que representa el error en la ingesta.
Deletion of Asrgl1 Leads to Photoreceptor Degeneration in Mice
Authors: Zhou Yu , Tian Wanli , Jiang Xiaoyan , Yang Huining , Jiang Zhilin , Li Xiao , Jiang Dan , Sun Kuanxiang , Yang Yeming , Liu Wenjing , Zhu Xianjun 
Published: January 2022

The asparaginase and isoaspartyl peptidase 1 (ASRGL1) is an L-asparaginase and beta-aspartyl peptidase enzyme that may be involved in the formation of L-aspartate, a neurotransmitter that can operate as an excitatory neurotransmitter in some brain regions. Although variants in ASRGL1 have been reported in retinitis pigmentosa (RP) patients, the in vivo functions and mechanisms of ASRGL in RP remains unknown due to the lack of suitable disease models. To explore the role of ASRGL in RP, we generated an Asrgl1 knockout mouse model (Asrgl1 KO) using the CRISPR/Cas9 technique. Asrgl1 ablation in mice led to an attenuated electroretinogram (ERG) response around 8 months. The thickness of the outer nuclei layer (ONL) started to decrease around 9 months in Asrgl1 KO mice and gradually intensified at 12 and 15 months. Immunostaining revealed thinner inner segment (IS) and thinner outer segment (OS) as well as the progressive degeneration of rod and cone cells in Asrgl1 KO mice. One hundred forty-nine transcriptional differentially expressed genes (DEGs) were found by RNA-seq in Asrgl1 KO retina. These DEGs were linked to a number of biological processes that were considerably enriched, including gastrointestinal disease and organismal injury and abnormalities. By analysis of canonical pathways, glucocorticoid receptor signaling was the most significant canonical pathway altered in Asrgl1 KO retina. Several molecules, including NFE2L2, IL-4, Foxp3, and Fos, were in the central nodes of the interaction network in Asrgl1 KO retina. In summary, our study provided a knockout mouse model for a better understanding of the molecular mechanism for ASRGL1-related RP.
Highly Efficient One-Step Protein Immobilization on Polymer Membranes Supported by Response Surface Methodology
Authors: Schmidt Martin , Abdul Latif Amira , Prager Andrea , Gläser Roger , Schulze Agnes 
Published: January 2022

Immobilization of proteins by covalent coupling to polymeric materials offers numerous excellent advantages for various applications, however, it is usually limited by coupling strategies, which are often too expensive or complex. In this study, an electron-beam-based process for covalent coupling of the model protein bovine serum albumin (BSA) onto polyvinylidene fluoride (PVDF) flat sheet membranes was investigated. Immobilization can be performed in a clean, fast, and continuous mode of operation without any additional chemicals involved. Using the Design of Experiments (DoE) approach, nine process factors were investigated for their influence on graft yield and homogeneity. The parameters could be reduced to only four highly significant factors: BSA concentration, impregnation method, impregnation time, and electron beam irradiation dose. Subsequently, optimization of the process was performed using the Response Surface Methodology (RSM). A one-step method was developed, resulting in a high BSA grafting yield of 955 mg m−2 and a relative standard deviation of 3.6%. High efficiency was demonstrated by reusing the impregnation solution five times consecutively without reducing the final BSA grafting yield. Comprehensive characterization was conducted by X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), and measurements of zeta potential, contact angle and surface free energy, as well as filtration performance. In addition, mechanical properties and morphology were examined using mercury porosimetry, tensile testing, and scanning electron microscopy (SEM).
Experimental Periodontitis Deteriorated Atherosclerosis Associated With Trimethylamine N-Oxide Metabolism in Mice
Authors: Xiao Lingling , Huang Lingyan , Zhou Xin , Zhao Dan , Wang Yan , Min Haiyan , Song Shiyu , Sun Weibin , Gao Qian , Hu Qingang , Xie Sijing 
Published: January 2022

BackgroundPeriodontitis is considered a risk factor for atherosclerosis, but the mechanism is not clear. It was reported that oral administration of Porphyromonas gingivalis altered the gut microbiota in mice. Gut dysbiosis and the intestinal metabolite trimethylamine N-oxide (TMAO) were verified to be associated with atherosclerosis. Therefore, the possible TMAO-related mechanism between periodontitis and atherosclerosis needs to be explored.MethodsExperimental periodontitis was established by oral administration of P. gingivalis for 2 months in ApoE−/− mice. Mouse hemi-mandibles were scanned using Micro-CT. Quantification of TMAO was performed using liquid chromatography–tandem mass spectrometry. Mouse feces were collected and the bacterial DNA was extracted, then the gut microbiota was analyzed using 16S rRNA genes. Atherosclerotic lesion areas were quantified. Livers, small intestines, and large intestines were analyzed for gene expression.ResultsAggravated atherosclerosis plaques were found in experimental periodontitis mice. Plasma TMAO, a pathogenic factor of atherosclerosis, was initially found to be increased in periodontitis mice. Changes in the composition and abundance of the intestinal microflora of periodontitis mice were found. Flavin monooxygenase 3 (FMO3), the catalyzing enzyme of TMAO in the liver, was significantly increased, accompanied by an increase of IL-6 in liver, the abnormal intestinal integrity and enhanced plasma LPS. The IL-6 and LPS were verified to be able to increase FMO3 in HepG2 cells.ConclusionOur research discovered that experimental periodontitis in ApoE−/− mice induced gut dysbiosis and an increase in TMAO. These results suggest a possible mechanism by which periodontitis may accelerate atherosclerosis by influencing the intestinal microbes and the metabolism, which were triggered by inflammation of the liver and intestine.
Is Partially Thrombosed False Lumen Really a Predictor for Adverse Events in Uncomplicated Type B Aortic Dissection: A Systematic Review and Meta-Analysis?
Authors: Wu Jinlin , Song Jian , Li Xin , Yang Jue , Yu Changjiang , Zhou Chenyu , Sun Tucheng , Fan Ruixin 
Published: January 2022

Objective:This meta-analysis and systematic review investigated whether partial thrombosed false lumen was a predictor for adverse events in uncomplicated Type B aortic dissection (TBAD).Methods:We performed the current systematic review of the medical literature according to the 2009 Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. The Newcastle-Ottawa Scale was used to evaluate the quality of individual studies. Search terms based on the MEDLINE database included “type B aortic dissection,” “false lumen” and “thrombosis.” The primary outcomes included mortality, intervention, and aortic growth.Results:Six studies were included in this systematic review, with a total number of 692 patients, including 197 patency (28.5%), 214 partial thrombosis (30.9%), and 281 complete thrombosis (40.6%). Due to the insufficient data for quantitative analysis, we only conducted a scoping review for mortality and intervention. For aortic growth, we conducted a meta-analysis based on Standardized Mean Difference (SMD). The SMD of PT vs. P by random effect model was −0.05 (random effect model) [95% confidence interval (CI), −0.39 to 0.29]. The 95% CI crossed with the null line of 0, indicating no significant difference. The SMD was 0.37 (fixed effects model) (95% CI, 0.03–0.71) and 0.70 (fixed effects model) (95% CI, 0.37–1.04) for PT vs. CT, and P vs. CT, respectively.Conclusions:Current researches on partial thrombosis of TBAD are inconsistent. Partial thrombosis is not associated with a faster aortic growth rate. Until more solid evidence is available, we do not recommend partial thrombosis as a surgical indication or high-risk profile for TBAD.Systematic Review Registration: Unique Identifier: CRD42019121912.
Daily Variations in pCO2 and fCO2 in a Subtropical Urbanizing Lake
Authors: Yang Rongjie , Chen Yingying , Du Jie , Pei Xiangjun , Li Jinghua , Zou Zan , Song Huixing 
Published: January 2022

The transfer of CO2 from lakes to the atmosphere is a component of the global carbon equilibrium, while the quantification of the CO2 partial pressure (pCO2) is critical for exploring the contribution of freshwater CO2 emissions in the regional/global carbon budget. To investigate the daily variability of pCO2 and CO2 fluxes (fCO2), we conducted in situ biweekly pCO2 detection at 7:00, 10:00, 14:00, and 17:00 China Standard Time (CST) from Jan. to Sept. 2020 in the subtropical urbanizing Qinglonghu Lake in Chengdu, Sichuan, China. The pCO2 during the daytime varied greatly from 8.3 to 1,061.3 μatm, with an average of 137.7 μatm, while the average pCO2 (n = 11) clearly gradually decreased from 7:00 CST (204.9 ± 295.7 μatm) to 17:00 CST (93.5 ± 105.5 μatm). Similarly, the average fCO2 values were −19.3 (±27.5), −24.8 (±20.7), −29.2 (±9.1) and −30.4 (±10.7) mmol m2 h−1 at 7:00–17:00 CST, respectively. Further, we observed a negative correlation between pCO2 and water temperature and dissolved oxygen, but a positive correlation between pCO2 and total organic carbon and chlorophyll a. By a systematic overview of previously published data, we also discussed the differences and uncertainties in pCO2 and fCO2 estimates at regional and global scales. We therefore speculate that uncertainties may exist in the contributions of CO2 balance on lake surface in regional/global carbon budgets due to this daily pCO2 variation.
hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p
Authors: Yan Yu-Xiang , Xiao Huan-Bo , Lu Ya-Ke , Sun Yue , Wang Shuo , Dong Jing , Wu Li-Juan 
Published: January 2022

IntroductionChronic stress plays an important role in the development of type 2 diabetes (T2D). Circular RNAs (circRNAs) play significant roles in regulating the pathogenesis of diseases by regulating gene expression. The aim of the present study was to identify the association between hsa_circ_0111707 and stress-related T2D.MethodsThe present study was performed based on a three-part design. The association between hsa_circ_0111707 in peripheral blood mononuclear cells (PBMCs) and T2D and stress-related variables were assessed in a cross-sectional study. The causal relationship of hsa_circ_0111707 on T2D was further investigated in a nested case-control study. miR-144-3p as the miRNA target of hsa_circ_0111707 was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.ResultsThe relative expression of hsa_circ_0111707 was significantly lower in the T2D and impaired fasting glucose (IFG) cases in comparison with controls. The hsa_circ_0111707 expression was significantly negatively correlated with miR-144-3p expression and plasma cortisol concentration and positively correlated with NR3C1 expression. In addition, hsa_circ_0111707 expression was negatively correlated with scores of “demands at work” and “insecurity at work” of Copenhagen Psychosocial Questionnaire (COPSOQ). Decreased hsa_circ_0111707 expression was associated with increased risk of T2D development. Functional analysis demonstrated that hsa_circ_0111707 functions as a sponge for miR-144-3p.Conclusionhsa_circ_0111707 is associated with risk of T2D development via sponging miR-144-3p. hsa_circ_0111707 in PBMCs can be considered a potential biomarker of stress-related T2D.
Acromegaly: The Research and Practical Value of Noninvasive Hemodynamic Assessments via Impedance Cardiography
Authors: Jurek Agnieszka , Krzesiński Paweł , Gielerak Grzegorz , Witek Przemysław , Zieliński Grzegorz , Kazimierczak Anna , Wierzbowski Robert , Banak Małgorzata , Uziębło-Życzkowska Beata 
Published: January 2022

BackgroundArterial hypertension (AH) that accompanies acromegaly (AC) may lead to cardiovascular dysfunction. Such consequences may be detected with impedance cardiography (ICG), which is a noninvasive method of hemodynamic assessment. Early detection of subclinical hemodynamic alterations in AC patients may be crucial for optimizing treatment and preventing cardiovascular remodeling. The purpose of this study was to identify the hemodynamic parameters of the cardiovascular system that differentiate patients with AC from those in the control group (CG), with a particular emphasis on potential targets for medical therapy.MethodsThis observational, prospective, clinical study involved a comparative analysis of 33 AC patients with no significant comorbidities and the controls selected via propensity score matching based on a set of baseline characteristics (age, sex, body mass index, mean blood pressure [MBP]), with comparable proportions of AH patients. The assessed hemodynamic parameters included the stroke volume index (SI), cardiac index, systemic vascular resistance index, velocity index (VI), acceleration index, Heather index (HI), and thoracic fluid content (TFC).ResultsBoth the AC group and the CG had well-controlled AH (mean blood pressure of 121/77 mmHg and 119/76 mmHg, respectively). In terms of baseline characteristics, the AC group was characterized by a higher hear rate and lower creatinine levels than the CG (76.2 bpm vs. 66.8 bpm [p = 0.001] and 0.755 mg/dL vs. 0.850 mg/dL [p = 0.035], respectively). ICG assessment of AC patients and CG patients showed the former to have higher heart rates (73.5 bpm vs. 65.2 bpm; p = 0.003), lower SI (43.8 mL/m2 vs. 53.4 mL/m2; p = 0.0001), lower VI (42.1 1/1000/s vs. 49.3 1/1000/s; p = 0.037), lower HI (8.49 Ohm/s2 vs. 13.4 Ohm/s2, p ≤ 0.0001), and higher thoracic fluid content (TFC) (38.4 1/kOhm vs. 28.1 1/kOhm; p ≤ 0.0001), respectively.ConclusionsEven with well-controlled hypertension, AC is associated with a high TFC, increased heart rate, and decreased indices of cardiac contractility. Hemodynamic changes in AC patients may be detected with the modern, noninvasive diagnostic tool, ICG.
Association Between Red Blood Cell Distribution Width and Thyroid Function
Authors: Zhou Guowei , Ai Yueqin , Guo Song , Chen Quan , Feng Xiao , Xu Kun , Wang Gaoyuan , Ma Chaoqun 
Published: January 2022

AimRed blood cell distribution width (RDW) is an important parameter with broad biological implications. However, the study investigating the association between RDW and thyroid function remains sparse and inconsistent. We aimed to investigate the association between RDW and thyroid function in the US population.MethodsA cross-sectional analysis was performed using the data from the National Health and Nutrition Examination Survey (NHANES) conducted from 2007 to 2010. The thyroid parameters investigated were mainly free triiodothyronine (fT3), free thyroxine (fT4), thyroid-stimulating hormone (TSH), antithyroglobulin antibody (TgAb), and antithyroperoxidase antibody (TPOAb). In the 6,895 adults aged 18 years or older, logistic regression modeling was applied to estimate the association between RDW quartiles and thyroid parameters. Smooth curve fittings and generalized additive models were then performed to address the nonlinear relationship.ResultsThe association between RDW and TSH followed a J-shaped curve, and a significant positive relationship existed in the 12.5%–17.5% range of RDW (β  = 0.350, 95% confidence interval (CI): 0.225 to 0.474), which was prominent in females. We further demonstrated a negative association (β = −0.018, 95% CI: −0.030 to −0.005) between RDW and fT3. Moreover, elevated RDW was more likely to be subclinical hypothyroidism. However, there was no obvious association between RDW and fT4.ConclusionThis study confirmed a significant association between RDW and TSH, and future studies are needed to elucidate the underlying mechanisms of the peculiar RDW-fT3 relationship. RDW may be a significant clinical marker of subclinical hypothyroidism.