bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cortical–hippocampal coupling during manifold exploration in motor cortex
Authors: Kim Jaekyung , Joshi Abhilasha , Frank Loren , Ganguly Karunesh 
Published: December 2022

AbstractSystems consolidation—a process for long-term memory stabilization—has been hypothesized to occur in two stages1–4. Whereas new memories require the hippocampus5–9, they become integrated into cortical networks over time10–12, making them independent of the hippocampus. How hippocampal–cortical dialogue precisely evolves during this and how cortical representations change in concert is unknown. Here, we use a skill learning task13,14 to monitor the dynamics of cross-area coupling during non-rapid eye movement sleep along with changes in primary motor cortex (M1) representational stability. Our results indicate that precise cross-area coupling between hippocampus, prefrontal cortex and M1 can demarcate two distinct stages of processing. We specifically find that each animal demonstrates a sharp increase in prefrontal cortex and M1 sleep slow oscillation coupling with stabilization of performance. This sharp increase then predicts a drop in hippocampal sharp-wave ripple (SWR)–M1 slow oscillation coupling—suggesting feedback to inform hippocampal disengagement and transition to a second stage. Notably, the first stage shows significant increases in hippocampal SWR–M1 slow oscillation coupling in the post-training sleep and is closely associated with rapid learning and variability of the M1 low-dimensional manifold. Strikingly, even after consolidation, inducing new manifold exploration by changing task parameters re-engages hippocampal–M1 coupling. We thus find evidence for dynamic hippocampal–cortical dialogue associated with manifold exploration during learning and adaptation.
Marine phytoplankton community data and corresponding environmental properties from eastern Norway, 1896–2020
Authors: Lundsør Elisabeth , Lømsland Evy Rigmor , Johnsen Torbjørn Martin , Engesmo Anette , King Andrew Luke , Staalstrøm Andrè , Norli Marit , Magnusson Jan , Sørensen Kai , Edvardsen Bente , Eikrem Wenche 
Published: December 2022

AbstractTime series are essential for studying the long-term effects of human impact and climatic changes on the natural environment. Although data exist, no long-term phytoplankton dataset for the Norwegian coastal area has been compiled and made publicly available in a standardised format. Here we report on a compilation of phytoplankton data from inner Oslofjorden going back more than a century. The database contains 605 sampling events from 1896 to 2020, and environmental data has also been provided when available. Although the sampling frequency has varied over time, the high taxonomic quality and relatively similar methodology make it very useful. For the last 15 years (2006–2020), the sampling frequency has been almost monthly throughout the year. This dataset can be used for time series analysis to understand community structure and changes over time. It can also be used to study common taxa’ responses to environmental variables and changes, seasonal or annual species diversity and be useful for developing ecological indicators.
Arbitrary entanglement of three qubits via linear optics
Authors: Blasiak Pawel , Borsuk Ewa , Markiewicz Marcin 
Published: December 2022

AbstractWe present a linear-optical scheme for generating an arbitrary state of three qubits. It requires only three independent particles in the input and post-selection of the coincidence type at the output. The success probability of the protocol is equal for any desired state. Furthermore, the optical design remains insensitive to particle statistics (bosons, fermions or anyons). This approach builds upon the no-touching paradigm, which demonstrates the utility of particle indistinguishability as a resource of entanglement for practical applications.
A comparative screening of laccase-mediator systems by white-rot fungi laccases for biocatalytic benzyl alcohol oxidation
Authors: Marino Ivana , Pignataro Eugenia , Danzi Donatella , Cellini Francesco , Cardellicchio Cosimo , Biundo Antonino , Pisano Isabella , Capozzi Maria Annunziata M. 
Published: December 2022

AbstractProduction of value-added compounds from waste materials is of utmost importance for the development of a sustainable society especially regarding their use as catalysts in industrially relevant synthetic reactions. Herein, we show the production of laccases from four white-rot fungi, which were grown on agricultural residues, specifically Trametes versicolor 11269, Pleurotus ostreatus 1020, Panus tigrinus 707 and Lentinula edodes SC-495. The produced laccases were tested on a laccase-mediator system (LMS) for the biocatalytic oxidation of the model substrate benzyl alcohol into benzaldehyde. The LMS was carried out in the presence both of tetrahydrofuran as co-solvent and of the mediator 2,2,6,6-tetramethyl-1-piperidinyloxyl (TEMPO) due to its high redox potential and its ability to perform the oxidation. Tolerance studies showed that the dialyzed solutions were able to tolerate 1% (99:1 v/v) of co-solvent, whereas a concentration of 10% v/v had a detrimental activity. Performances in the biocatalytic oxidation of laccase solutions from different purification steps were compared. Similar conversion was observed for laccase in dialysis (raw) and gel filtration (GF) product versus commercial T. versicolor laccase. The latter oxidized almost 99% of substrate while the other laccase solutions were able to reach a conversion from 91% for the laccase solution from P. tigrinus 707 after dialysis, to 50% for the laccase solution from P. ostreatus 1020 after gel filtration. This work highlights the potential of unpurified laccase solutions to be used as catalysts in synthetic reactions.
Characterization of Boeremia exigua causing stem necrotic lesions on Luobuma in northwest China
Authors: Lan Yanru , Duan Tingyu 
Published: December 2022

AbstractLuobuma (Apocynum venetum, Poacynum pictum, and P. hendersonni) are perennial herbs widely used in the textile and medical industries and ecological restoration. In the summer of 2020, reddish-brown or off-white sunken shape necrotic lesions were observed on the stems and shoots of seven Luobuma ecotypes grown in the field in Yuzhong County, Gansu province of China, which is a limiting factor that affects the growth, function and application of Luobuma. To make clear whether the new symptoms were caused by a novel pathogen, a combined research in field and greenhouse was conducted. Based on the morphological and molecular analysis results, the pathogen causing the necrotic lesions was identified as Boeremia exigua var. rhapontica. The incidence and disease index of the seven ecotypes in the field ranged from 11.49 to 33.68% and 6.63 to 23.01, respectively, from 2020 to 2021. The results showed that the disease severity gradually increased with the growing season. According to the pathogenicity analysis of the eight ecotypes in the greenhouse, the ecotypes Pp-BMK and Pp-BMH were susceptible, while ecotype Pp-BMQ was resistant to Boeremia exigua var. rhapontica infection. Thus, the present study provides a theoretical basis for preventing and controlling the stem and leaf necrotic lesions disease on Luobuma by planting resistant varieties/ecotypes. To our knowledge, this is the first report of stem necrotic lesions and leaf spots on Luobuma caused by B. exigua var. rhapontica.
Effect of social support on anxiety of medical staff one year after COVID-19 outbreak: a moderated mediating model
Authors: Wang Bin , Zhong Xiao , Fu Haojie , Hu Ruilin , He Mengting , Zhang Guanzi 
Published: December 2022

AbstractOne year after the outbreak of COVID-19, medical staff are facing high anxiety due to multiple work stresses. Social support has become a protective factor for healthcare workers' anxiety symptoms, but the exact mechanism of action is not yet known. Based on the buffering model, this study aims to explore the impact and mechanisms of action of healthcare workers' perception of social support (PSS) on anxiety symptoms in the context of the epidemic and to further explore how the emotional characteristics of risk perception (ECRP) and resilience play their influence. To this end, this study measured 839 healthcare workers using an online questionnaire from 4 February to 1 March 2021. The results found that PSS among healthcare workers negatively predicted anxiety symptoms. ECRP partially mediated the relationship between PSS and anxiety symptoms, and resilience moderated the first half of the pathway in the model of PSS through ECRP on anxiety symptoms. The emotional characteristics of risk perception of COVID-19 in individuals with high resilience decrease significantly with the increase of PSS, while this change is not significant in individuals with low resilience.
The development of audio–visual temporal precision precedes its rapid recalibration
Authors: Han Shui’er , Chen Yi-Chuan , Maurer Daphne , Shore David I. , Lewis Terri L. , Stanley Brendan M. , Alais David 
Published: December 2022

AbstractThrough development, multisensory systems reach a balance between stability and flexibility: the systems integrate optimally cross-modal signals from the same events, while remaining adaptive to environmental changes. Is continuous intersensory recalibration required to shape optimal integration mechanisms, or does multisensory integration develop prior to recalibration? Here, we examined the development of multisensory integration and rapid recalibration in the temporal domain by re-analyzing published datasets for audio–visual, audio–tactile, and visual–tactile combinations. Results showed that children reach an adult level of precision in audio–visual simultaneity perception and show the first sign of rapid recalibration at 9 years of age. In contrast, there was very weak rapid recalibration for other cross-modal combinations at all ages, even when adult levels of temporal precision had developed. Thus, the development of audio–visual rapid recalibration appears to require the maturation of temporal precision. It may serve to accommodate distance-dependent travel time differences between light and sound.
The release of osteoclast-stimulating factors on supraphysiological loading by osteoprogenitors coincides with expression of genes associated with inflammation and cytoskeletal arrangement
Authors: Bratengeier Cornelia , Bakker Astrid D. , Liszka Aneta , Schilcher Jörg , Fahlgren Anna 
Published: December 2022

AbstractSupraphysiological loading induced by unstable orthopedic implants initiates osteoclast formation, which results in bone degradation. We aimed to investigate which mechanosensitive cells in the peri-implant environment produce osteoclast-stimulating factors and how the production of these factors is stimulated by supraphysiological loading. The release of osteoclast-stimulating factors by different types of isolated bone marrow-derived hematopoietic and mesenchymal stem cells from six osteoarthritic patients was analyzed after one hour of supraphysiological loading (3.0 ± 0.2 Pa, 1 Hz) by adding their conditioned medium to osteoclast precursors. Monocytes produced factors that enhanced osteoclastogenesis by 1.6 ± 0.07-fold and mesenchymal stem cells by 1.4 ± 0.07-fold. Medium from osteoprogenitors and pre-osteoblasts enhanced osteoclastogenesis by 1.3 ± 0.09-fold and 1.4 ± 0.03-fold, respectively, where medium from four patients elicited a response and two did not. Next generation sequencing analysis of osteoprogenitors revealed that genes encoding for inflammation-related pathways and cytoskeletal rearrangements were regulated differently between responders and non-responders. Our data suggest that released osteoclast-stimulating soluble factors by progenitor cells in the bone marrow after supraphysiological loading may be related to cytoskeletal arrangement in an inflammatory environment. This connection could be relevant to better understand the aseptic loosening process of orthopedic implants.
Investigating interactions between types of order in categorization
Authors: Mezzadri Giulia , Reynaud-Bouret Patricia , Laloë Thomas , Mathy Fabien 
Published: December 2022

AbstractThis study simultaneously manipulates within-category (rule-based vs. similarity-based), between-category (blocked vs. interleaved), and across-blocks (constant vs. variable) orders to investigate how different types of presentation order interact with one another. With regard to within-category orders, stimuli were presented either in a “rule plus exceptions” fashion (in the rule-based order) or by maximizing the similarity between contiguous examples (in the similarity-based order). As for the between-category manipulation, categories were either blocked (in the blocked order) or alternated (in the interleaved order). Finally, the sequence of stimuli was either repeated (in the constant order) or varied (in the variable order) across blocks. This research offers a novel approach through both an individual and concurrent analysis of the studied factors, with the investigation of across-blocks manipulations being unprecedented. We found a significant interaction between within-category and across-blocks orders, as well as between between-category and across-blocks orders. In particular, the combination similarity-based + variable orders was the most detrimental, whereas the combination blocked + constant was the most beneficial. We also found a main effect of across-blocks manipulation, with faster learning in the constant order as compared to the variable one. With regard to the classification of novel stimuli, learners in the rule-based and interleaved orders showed generalization patterns that were more consistent with a specific rule-based strategy, as compared to learners in the similarity-based and blocked orders, respectively. This study shows that different types of order can interact in a subtle fashion and thus should not be considered in isolation.
Design, synthesis, in vitro, in silico, and SAR studies of flavone analogs towards anti-dengue activity
Authors: Patigo Apinya , Hengphasatporn Kowit , Cao Van , Paunrat Wattamon , Vijara Natthanan , Chokmahasarn Thamonwan , Maitarad Phornphimon , Rungrotmongkol Thanyada , Shigeta Yasuteru , Boonyasuppayakorn Siwaporn , Khotavivattana Tanatorn 
Published: December 2022

AbstractFlavone has recently been proved as a promising scaffold for the development of a novel drug against dengue fever, one of the major health threats globally. However, the structure–activity relationship study of flavones on the anti-dengue activity remains mostly limited to the natural-occuring analogs. Herein, 27 flavone analogs were successfully synthesized, of which 5 analogs (5e, 5h, 5o, 5q, and 5r) were novel. In total, 33 analogs bearing a diverse range of substituents were evaluated for their efficacy against DENV2-infected LLC/MK2 cells. The introduction of electron-withdrawing groups on ring B such as Br (5m) or NO2 (5n and 5q) enhanced the activity significantly. In particular, the tri-ester 5d and di-ester 5e exhibited low toxicity against normal cell, and exceptional DENV2 inhibition with the EC50 as low as 70 and 68 nM, respectively, which is over 300-fold more active compared to the original baicalein reference. The viral targets for these potent flavone analogs were predicted to be NS5 MTase and NS5 RdRp, as suggested by the likelihood ratios from the molecular docking study. The great binding interaction energy of 8-bromobaicalein (5f) confirms the anti-dengue activity at atomistic level. The physicochemical property of all the synthetic flavone analogs in this study were predicted to be within the acceptable range. Moreover, the QSAR model showed the strong correlation between the anti-dengue activity and the selected molecular descriptors. This study emphasizes the great potential of flavone as a core structure for further development as a novel anti-dengue agent in the future.
Evaluation of formaldehyde, particulate matters 2.5 and 10 emitted to a 3D printing workspace based on ventilation
Authors: Kim Taehun , Hong Dayeong , Moon Sojin , Kim Namkug 
Published: December 2022

AbstractRecently, the development of 3D printing (3DP) technology and its application in various fields have improved our quality of life. However, hazardous materials that affect the human body, such as formaldehyde and particulate matter (PM), are emitted into the air during 3DP. This study measured the formaldehyde, PM10, and PM2.5 emitted by 3DP with the ventilation operation using six materials in material extrusion (ME) and vat photopolymerization (VP) and compared them between the 3DP workspace and the control setting with test–retest validation by two researchers. The experiments were divided into four stages based on the 3DP and ventilation operation. A linear mixed model was used to analyze the mean differences and tendencies between the 3DP workspace and the control setting. The change as ventilation was switched from off to on was evaluated by calculating the area. The differences and tendencies were shown in the statistically significant differences from a post-hoc test (α = 0.0125) except for some cases. There was a significant difference in formaldehyde depending on the ventilation operation; however, only a minor difference in PM10, and PM2.5 was confirmed. The amount of formaldehyde exceeding the standard was measured in all materials during 3DP without ventilation. Therefore, it is recommended to operate ventilation systems.
A lower sleep regularity index (SRI) is associated with relapse in individuals with alcohol use disorder following inpatient treatment
Authors: Barb Jennifer J. , Brooks Alyssa T. , Kazmi Narjis , Yang Li , Chakravorty Subhajit , Wallen Gwenyth R. 
Published: December 2022

AbstractThe sleep regularity index (SRI) is used to measure an individual’s sleep/wake consistency over time. The SRI has been associated with certain health risks; to date, research investigating the relationship between the SRI and relapse in individuals with alcohol use disorder (AUD) is lacking. The aim of this work was to evaluate the SRI and relapse in individuals with AUD following inpatient treatment. Individuals with AUD (n = 77, mean age = 49.5 ± 10.86) were assessed for 28-days following discharge from an inpatient treatment program. Logistic regression was applied to examine the impact of SRI on relapse as the outcome variable of interest. Sleep quality was lower in individuals who relapsed compared to those who did not. Moreover, SRI scores were significantly worse in those who relapsed compared to those who did not. Over the entire patient cohort, lower weekly SRI scores were significantly correlated with longer weekly nap duration. Logistic regression model results indicated that the overall SRI was a significant predictor of relapse. The SRI represents a relevant aspect of sleep health and should be considered when assessing an individual’s sleeping patterns. Behavior based interventions related to the importance of individualized consistency in sleep and wake patterns may be particularly important for treatment seeking individuals with AUD not only during inpatient treatment, but also once these individuals have transitioned into their outpatient phase of recovery. These findings support the notion of SRI as a separate facet of sleep health worth investigating in at-risk, disease specific groups.
Purification of aquaculture effluent using Picralima nitida seeds
Authors: Igwegbe Chinenye Adaobi , Ovuoraye Prosper Eguono , Białowiec Andrzej , Okpala Charles Odilichukwu R. , Onukwuli Okechukwu Dominic , Dehghani Mohammad Hadi 
Published: December 2022

AbstractAquaculture effluent treatment is essential to eliminate the undesirable characteristics of water to ensure cleaner production and environmental sustainability. In an effort to develop green coagulant without compromising cost, this research investigated the feasibility of aquaculture effluent (AQEF) pollutant removal usingPicralima nitidaseeds extract (PNSC) and its bio-coagulation/adsorption kinetic characteristics with the substrate in water. The coagulative decrease was observed in terms of TD (turbidity), TSS (total suspended solids), COD (chemical oxygen demand), BOD (biochemical oxygen demand), and COLR (color) from AQEF. The active coagulant was extracted from the seeds and analyzed for its spectral and morphological characteristics through FTIR and SEM. The influence of PNSC dosage (0.10–0.50 g L−1), pH (2–10), settling time (0–60 min), and temperature (303–323 K) on the removal of contaminants were surveyed. The process kinetics of coagulation–flocculation were also explored. Maximal TD reduction of 90.35%, COD (82.11%), BOD (82.38%); TSS (88.84%), and COLR (65.77%) at 0.2 g PNSC L−1, pH 4, and 303 K was achieved. Analysis of variance (ANOVA) tests proved that pH, temperature, and settling time had a significant effect on pollutant removal. Results fitted Von Smoluchowski’s perikinetics theory at the optimum conditions, which gave R2 > 0.900. At perikinetics circumstances, the Kb (reaction rate) and $${t}_{f\frac{1}{2}}$$tf12 (half-life) correspond to 0.0635 Lg−1 min−1 and 1.9 min. More so, sorption results fitted the Lagergren over the Ho model. Additionally, the net cost of using PNSC to handle 1 L of AQEF (including electricity, material, and labor costs) was evaluated to be €4.81. Overall, the PNSC appears reliable and useful in pretreating AQEF for improved biodegradability and superior effluent quality.
Dynamics of rumen microbiome in sika deer (Cervus nippon yakushimae) from unique subtropical ecosystem in Yakushima Island, Japan
Authors: Eto Misaki , Yahara Tetsukazu , Kuroiwa Arika , Shioya Katsunori , Flores Gilberto E. , Hamamura Natsuko 
Published: December 2022

AbstractYaku sika deer (Cervus nippon yakushimae) are endemic to Yakushima Island, whose landscape covered with primary evergreen forest is recognized as a World Heritage Site. In this study, the rumen bacterial microbiota (RBM) of wild Yaku sika was characterized using high throughput sequencing of bacterial 16S rRNA genes combined with targeted cultivation and functional analyses. Comparative analyses of RBM datasets from other ruminant animals revealed distinct community structure among domesticated and wild ruminants. Wild Yaku sika RBM exhibited higher species richness than other sika deer (i.e. wild Ezo sika and domesticated sika deer), likely reflecting their dietary variations associated with unique ecosystem in the island. The Yaku sika RBM of high deer population density samples exhibited higher diversity and contained higher proportion of Firmicutes than those of lower density samples. Moreover, the highest abundance of tannase gene were observed in individuals from the highest population density area, consistent with the previous observation that Yaku sika in the high density areas expanded their feed to include tannin-rich unpalatable plants. This study indicated that RBM of unique wild Yaku sika contribute to the flexibility of dietary shift and thus maintaining nutritional status of Yaku sika under high density conditions.