bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nationwide Survey on Communication Skills (CS) Training of Physicians in China and its Role in Actual Challenges of Patient-Physician-Relationship
Authors: Junfeng Du , Mayer Gwendolyn , Posenato Elisabetta , Hummel Svenja , Zafar Ali , Bärnighausen Till , Schultz Jobst-Hendrik 
Published: May 2022

Abstract
Background
The Chinese healthcare system is affected by frequent disputes between physicians and patients. Although recent reforms have contributed towards improving the patient-physician relationship, distrust in physicians is still high. Communication skills (CS) training of physicians holds the key to improving patient confidence and diffusing stressful situations. This nationwide survey reports on the status of CS training in medical education in China, and the experiences and attitudes of physicians towards CS training.
Methods
A cross-sectional survey was conducted at medical institutions across China. A questionnaire developed for this study included the status of CS training, current aspects of patient-physician relationships, perceived own CS and patient-centeredness with Likert-scaled items from 1 (most negative) to 6 (most positive). Physicians’ attitude towards CS training was measured with the Communication Skills Attitude Scale (CSAS) and its subscales PAS (Positive Attitude Scale) and NAS (Negative Attitude Scale). Data were analyzed descriptively and for group differences between the hospital level and operating vs. non-operating physicians. Binary logistic regression analysis was done to find associations explaining the occurrence of verbal and physical attacks and the role of CS attitudes.
Results
Out of 1.080 questionnaires, 772 physicians met inclusion criteria. A total of 466/772 participants (60.4%) had received at least one CS training during their career. The participants rated the current situation related to patient-physician relationship in China as highly stressful (mean = 4.52, SD = 1.26, 95% CI: 4.43–4.60), experiencing verbal attacks in the past three years once a year in 372/772 cases (48.2%) and physical attacks 111/772 times (14.4%). The mean PAS was 62.96 (SD = 7.63, 95% CI: 62.41–63.47) indicating a high positive attitude towards CS training. Being female was associated with an increased risk of verbal attacks (OR = 1.51, 95% CI: 1.01–2.25) while working in a tertiary hospital and showing high levels of PAS decreased this risk (OR = 0.62, 95% CI: 0.43–0.89, and OR = 0.95, 95% CI: 0.93–0.98). Having received a previous training decreased the odds of physical attacks (OR = 0.54, 95% CI: 0.35–0.83).
Conclusions
A majority of Chinese physicians were trained in CS and would value further training. Our results highlight that CS training is likely to promote patient-centered communication and reduce attacks against physicians. Both of these effects are to improve the patient-physician-relationship in the long run. More CS training should be offered to Chinese physicians, especially at secondary- and primary-care hospitals, where such practices remain infrequent.
WITHDRAWN: Transcriptome analysis reveals hepatotoxicity in zebrafish induced by cyhalofop-butyl
Authors: Liu Tingting , Zhou Tao , Yang Yang 
Published: May 2022

Abstract

Cyhalofop-butyl is a highly effective aryloxyphenoxypropionate herbicide and widely used for weed control in paddy fields. With the increasing residue of herbicides, it poses a threat to the survival of aquatic organisms. Here, we evaluated the effect of cyhalofop-butyl on zebrafish to explore its potential hepatotoxic mechanism. The results showed that cyhalofop-butyl (0.1, 0.2 and 0.4 mg/L) induced hepatocyte degeneration, vacuolation and necrosis of larvae after embryonic exposure for 4 days and caused liver atrophy after exposure for 5 days. Meanwhile, the activities of enzymes related to liver function named alanine transaminase (ALT), aspartate transaminase (AST) were significantly increased by 0.2 mg/L cyhalofop-butyl and higher. And the contents of triglyceride (TG) involved in lipid metabolism was significantly decreased by 0.4 mg/L cyhalofop-buty. The effects of cyhalofop-butyl on zebrafish larvae were further demonstrated by GO (Gene Ontology) and KEGG pathway analysis. Cyhalofop-butyl (0.1, 0.2, 0.4 mg/L) altered the expression of 116, 154, 397 genes in liver，these genes are mainly enriched in metabolism (such as lipid metabolism, amino acid metabolism), immune system (Toll-like receptor signaling pathway) and endocrine system (PPAR signaling pathway). Furthermore, the expression of key genes related to liver development combined with RNA-Seq results, indicated that cyhalofop-butyl might damage the liver development of zebrafish larvae and cause metabolic disorders. To sum up, our research results reveal the physiological and molecular responses of zebrafish liver to cyhalofop-butyl and provide new insights for further studying the mechanism of cyhalofop-butyl toxicity to aquatic organisms.
Metabolic effects of dopamine agonists treatment among patients with prolactinomas.
Authors: Kabootari Maryam , Shirmohammadli Habibeh , Golgiri Fatemeh , Mosalamiaghili Seyedarad , Khajavi Alireza , Akbari Hamideh 
Published: May 2022

Abstract
Purpose: To evaluate the effect of cabergoline treatment on metabolic parameters including the Triglyceride-glucose (TyG) index in newly diagnosed patients with prolactinoma.Methods: 71 consecutive non-diabetic patients with prolactinoma were enrolled. Body mass index (BMI), blood pressure, and laboratory tests including TyG index were measured for each patient. Then, treatment with cabergoline was started for each patient and continued for 6 months. Anthropometric and laboratory tests were repeated every 3 months to evaluate the effects of cabergoline treatment. Results: At the baseline examination, the mean (SD) age, BMI, waist circumference (WC), systolic blood pressure (SBP), and diastolic blood pressure (DBP) of patients were 36.2 (10.5) years, 29.2 (5.0) kg/m2, 98.2 (13.7) cm, 115.3 (13.3) mmHg, and 71.4 (8.1) mmHg, respectively. Furthermore, men were older and had higher values for BMI, WC, SBP, and metabolic syndrome (MetS) prevalence compared to women. Cabergoline treatment significantly improved anthropometric and metabolic measures including BMI, WC, fasting plasma glucose, triglycerides, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, uric acid (only among women), TyG index, and hypogonadism, while no significant change was detected in SBP and DBP. No significant change was found in Mets syndrome prevalence among women whereas the declining trend from baseline to 3-month evaluation was significant among men. Conclusion: Short-term treatment with cabergoline can significantly improve cardiovascular risk factors except for blood pressure. Moreover, the TyG index as a surrogate marker of insulin resistance decreased significantly after the reduction of prolactin by treatment. Generally, results were similar among both genders.
In silico screening of Daphne tangutica Maxim plant for their bio-active phytochemicals as type 2 diabetes potential inhibitors
Authors: Nazir Hafiza Ayesha , Shafiq Nusrat , Arshad Uzma , Zameer Rabia , Yasmin Farah , Imtiaz Fazeelat , Rashid Maryam , Parveen Shagufta , Nisar Bushra , Khan Jallat 
Published: May 2022

Abstract
Ethnopharmacological relevance: Previously before the invention of virtual screening methods or techniques almost 80% of drugs were obtained from natural resources or compounds obtained from these sources. Plant species of the genus Daphne have historical background as a source of bioactive phytochemicals such as flavonoids including daphnodorins which have been found to possess significant chemotaxonomic value in drug discovery. Aim of the study: Their comprehensive pharmacological, phytochemical, biological, various catalytic activities and clinical uses have prompted us to conduct the optimization and structure based virtual screening of its potent analogues. The aim of this work is to investigate the use of daphnodorins analogues for the first time as anti-diabetic inhibitors on the basis of significant features by computational analysis.Materials and methods: A dataset of 38 compounds was selected from authentic database i.e., PubChem and ZINC database for computational analysis and quantitative structure activity relationship (QSAR). Optimized compounds were docked against various co-crystallized structures of inhibitors, antagonists and receptors were downloaded from PDB by implicating AutoDock Vina, Discovery studio 2020, PYMOL (Schrodinger). After performing molecular docking compounds of desired threshold were analyzed by ADMET studies through Swiss ADMET, ADMET SAR, ADMET lab and ProTox-II for investigating their drug-like nature. Elaboration of electronic structures of hit compounds was carried out using Gaussian 09W by intimating Density Functional Theory (DFT).Results: At significant phases of drug design approaches regular use of molecular docking has helped to promote the separation of important representatives from 38 pharmaceutically active compounds and 14 compounds were selected as lead compounds which were analyzed through ADMET studies for identifying their drug like properties and toxicity. Dataset of these 14 hit compounds was subjected for measuring significant parameters such as dipole moment, electronic energy, energy of Frontier Molecular Orbitals (FMO) and hardness of molecules by performing DFT as a result, one compound was selected as lead compound for anti-diabetic agent.
Association between maternal employment and the child's mental health: a systematic review with meta-analysis
Authors: Kopp Marie , Lindauer Marina , Garthus-Niegel Susan 
Published: May 2022

Abstract
Introduction: Especially during infancy and early childhood the role of maternal employment for child mental health problems has been investigated. This systematic review with meta-analysis covers very recent evidence on the variables’ of interest association. Methods: A systematic literature search was conducted. Studies had to compare children 0–7 years of age dependent on their mothers’ employment status, working amount, employment duration, or timing. Behavior problems, prosocial behavior, and subordinate measures, e.g., aggression, constituted child mental health outcomes. Following qualitative syntheses of evidence for the outcomes under investigation respective meta-analyses were conducted, if possible. Results: Being employed was associated with more conduct problems but less internalizing behavior problems; full-time employment was linked to more externalizing behavior problems and more hyperactivity/inattention. Longer employment duration was related to less (internalizing) behavior problems and more prosocial behavior but also more externalizing behavior problems. Qualitative syntheses indicated early maternal return to work to be associated with more externalizing behavior problems and less prosocial behavior. Discussion: Whether maternal employment is associated with child mental health strongly depends on the operationalization of maternal employment and the outcome under investigation. Especially part-time employment, longer employment duration, and return to work only after the first year postpartum may be beneficial for child mental health. Practical implications pertain an expanded offer of family leave and to the endorsement of maternal employment after the first year postpartum under consideration of factors that may buffer the negative associations with full-time employment.
The effect of temperature field on the characteristics of carbon fiber reinforced thermoplastic composites in the laying and shaping process
Authors: Zhao Fuhai , Liu Zhiqiang , Chen Rifan , Hao Yi , Ma Zhihao 
Published: May 2022

Abstract
The temperature field during the automated lay-up of carbon fiber reinforced thermoplastic composites is investigated in this work. The heat transfer boundary conditions of the lay-up process are determined by establishing a mathematical model of the temperature field; the basic properties of the material are determined using CF/PEEK as the research object; the heat transfer parameters in the temperature field are analyzed to determine the thermal convection region affected by hot air and the radiated heat of the infrared lamp; and a finite element model of the temperature field is established.The FEM temperature field model was created to investigate heat transfer throughout the lay-up process, as well as the impact of mold heating, lay-up preheating, and varied heating temperatures on different lay-ups. The temperature change law of different lay-ups under different heating and preheating temperatures of hot air is analyzed; the online temperature measurement device is built, and the automatic lay-up experiment is carried out to analyze the heating performance of infrared lamp and hot air gun and to determine their relevant parameters.
Using the results of experiments and simulations, it has been determined that different mold temperatures are required for different lay-ups; preheating can effectively reduce the temperature gradient between lay-ups; and at a lay-up speed of 5mm/s, a preheating temperature of 200°C and a hot air temperature above 400°C can ensure that the critical temperature between the prepreg wire bundle and lay-ups reaches the melting point and can be laid up normally.
Research on Characteristics and Influencing Factors of the Spatial Distribution of the ICG in the Yellow River Basin of China
Authors: Nie Xin , xie Yong , xie Xiaoxiao , Zheng Lunxing 
Published: May 2022

Abstract
This paper explores the characteristics and influencing factors of the spatial distribution of 889 national intangible cultural heritage in the Yellow River basin of China based on ArcGIS spatial analysis and Geodetector. The results show that the distribution of national intangible cultural heritage items in the Yellow River basin is significantly different among provinces, and most of them are distributed in central and western regions. From east to west, the density of intangible heritage in the Yellow River basin presents a "triangular" pattern of "one area and three points". All kinds of intangible cultural heritage in the Yellow River basin generally show the characteristic of aggregated distribution. Traditional skills, traditional medicine, traditional theatre, traditional music, and folklore have high levels of agglomeration, and traditional dance, Quyi, folk literature, and traditional fine arts have relatively lower levels. The level of traditional sports, entertainment and acrobatics is the lowest. Among social and humanistic factors, highway density is the most influential indicator for the spatial distribution of national intangible cultural heritage in the Yellow River basin. The GDP, population density and urbanization level also have a great impact on the spatial distribution of national intangible cultural heritage in the Yellow River basin. Among geographical environment factors, the river system and topography have a certain effect on the spatial distribution of national intangible cultural heritage in the Yellow River basin. Based on those facts, this paper finally discusses the specific path to protect and develop intangible cultural heritage in the Yellow River basin under the background of the new era, in order to promote its creative protection and innovative development.
Distinguished prognostic value of tumor-infiltrating lymphocytes in schistosomal and non-schistosomal colorectal cancer
Authors: Wang Weixia , Zhang Yingyi , Liu Jican , Jing Hongyan , Lu Kui , Wang Limei , Zhu Ting , Xu Yanchao , Bu Dacheng , Cheng Meihong , Liu Jing , Shen Weidong , Yao Junxia , Huang Sinian 
Published: May 2022

Abstract
Aim: To investigate the relationship between schistosomiasis and tumour-infiltrating lymphocytes (TILs), and the prognostic value of the TILs in schistosomal colorectal cancer (CRC).Background: Although schistosomiasis has been basically eliminated, it has not been completely extinction in China and occasional outbreaks occur in Europe recently. The relationship between schistosomiasis and CRC is still obscure, and the inflammation based prognostic systems of schistosomal colorectal (SCRC) has rarely been reported.Methods: HE-stained sections of 351 CRC tumours, which were completely resected, were evaluated for density of total TILs (tTILs). Meanwhile, CD3+T lymphocytes and CD20+B lymphocytes were detected by immunochemistry. The relationship of these infiltrating immune cells with clinicopathological features, including schistosomiasis, and clinical outcomes were evaluated and the prognostic role of TILs in SCRC was explored.Results: Total TILs were negatively correlated with tumor size，pathological T stage, lymph node metastasis and number of tumor budding (p＜0.05). CD3+ T cell density was also inversely associated with tumor size, tumor budding and pathological T stage(p＜0.05). CD20+ B cells density was correlated with colonic perforin (p=0.034). In the whole cohort, multivariate analysis identified Schistosomiasis, tTILs and CD3 and CD20 as independent prognostic factors (p < 0.05). Patients were divided into two groups based on schistosomal infection status: Schistosomal CRC (SCRC set) and Non-schistosomal CRC (NSCRC set). Both tTILs (p=0.009) and CD3 (p=0.002) were independent prognostic factors in the NSCRC set. However, merely CD3 (p=0.0016) was independent predictor in the SCRC set. Conclusion: The prognostic roles of total TILs, and CD3+ T and CD20+ B cell were different in CRC patients with and without schistosomiasis, suggesting distinguished roles in the immune microenvironment in SCRC and NSCRC patients.
Development of microbial communities in biofilm and activated sludge in a hybrid reactor
Authors: Godzieba Martyna , Zubrowska-Sudol Monika , Walczak Justyna , Ciesielski Slawomir 
Published: May 2022

Abstract
Microorganisms play a key role in biological wastewater treatment. The form in which biomass develops is related to the structure of its microbiome and determines the efficiency of organic compound conversion. However, the results of studies comparing the microbial communities in biofilm and activated sludge are often conflicting. Therefore, the objective of this study was to compare the composition and development of the bacterial communities in biofilm and activated sludge in a hybrid reactor. The Chao1 and Shannon biodiversity indices indicated that the biofilm bacterial community is richer and more diverse than the activated sludge community. The bacterial composition of the biofilm differed from that of the suspended biomass, although some core groups of bacteria (Proteobacteria, Bacteroidota, Actinobacteriota and Acidobacteriota) were highly abundant in both types of biomass. The biofilm was more diverse in terms of bacterial composition and a better environment for the development of nitrifiers (e.g., Nitrospira, Nitrosomonas) and phosphorus accumulating organisms (Candidatus Accumulibacter). Bacteria in the biofilm network had more connections with each other than those in the suspended biomass network. These results indicate that the genera present in the biofilm interact with each other to a greater degree than those in the suspended biomass.
Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural muscle
Authors: Melnikov Ivan Y. , Tyganov Sergey A. , Sharlo Kristina A. , Ulanova Anna D. , Vikhlyantsev Ivan M. , Mirzoev Timur M. , Shenkman Boris S. 
Published: May 2022

Abstract
In mammals, prolonged mechanical unloading results in a significant decrease in passive stiffness of postural muscles. The nature of this phenomenon remains unclear. The aim of the present study was to investigate possible causes for a reduction in rat soleus passive stiffness after 7 and 14 days of unloading (hindlimb suspension, HS). We hypothesized that HS-induced decrease in passive stiffness would be associated with calpain-dependent degradation of cytoskeletal proteins or a decrease in actomyosin interaction. Wistar rats were subjected to HS for 7 and 14 days with or without PD150606 (calpain inhibitor) treatment. Soleus muscles were subjected to biochemical studies and ex vivo measurements of passive tension with or without blebbistatin treatment (an inhibitor of actomyosin interactions). Passive tension of isolated soleus muscle significantly reduced after 7- and 14-day HS compared to control values. PD150606 treatment during 7- and 14-day HS induced an increase in alpha-actinin-2 and − 3, desmin contents compared to control, partly prevented a decrease in intact titin (T1) content and prevented a decrease in soleus passive tension. Incubation of soleus muscle with blebbistatin did not affect HS-induced reductions in specific passive tension in soleus muscle. Our study suggests that calpain-dependent breakdown of cytoskeletal proteins, but not a change in actomyosin interaction, significantly contributes to unloading-induced reductions in intrinsic passive stiffness of rat soleus muscle.
One-stage posterior surgery combined with anti-Brucella therapy in the management of lumbosacral brucellosis spondylitis: a retrospective study
Authors: Luan Haopeng , Liu Kai , Deng Xiaonan , Sheng Weibin , Maimaiti Maierdan , Guo Hailong , Li Huaqiang , Deng Qiang 
Published: May 2022

Abstract
Background
This study aimed to assess the clinical efficacy of one-stage posterior surgery combined with anti-Brucella therapy in the treatment of lumbosacral brucellosis spondylitis (LBS).
Methods
From June 2010 to June 2020, the Clinical and radiographic data of patients with LBS treated by one-stage posterior surgery combined with anti-Brucella therapy were retrospectively analyzed. The Visual Analogue Scale (VAS), Oswestry Disability Index (ODI), Frankel classification system, Japanese Orthopaedic Association (JOA) scale, and Bridwell grading system were utilized to evaluate the results of preoperative and postoperative recovery.
Results
A total of 55 patients who met the criteria were included in this study with a mean postoperative follow-up time of 2.6 ± 0.8 years. There were 40 males and 15 females with a mean age of 39.8 ± 14.7 years. The preoperative Brucella agglutination test was ≥ 1: 160 in all patients, but the blood culture was positive in 43 patients (78.1%). ESR and CRP returned to normal at 3 postoperative months, and a statistical difference was observed between the preoperative vs. three postoperative months (P < 0.05), and the preoperative vs. final follow-up (P < 0.05). VAS, ODI, and JOA scores of the preoperative were improved after surgery, there was a statistical difference in the comparison with three postoperative months, and final follow-up (P < 0.05). The satisfactory recovery of lower limb motor function was received in 53 patients (96.3%) after surgery. None of the infestation recurrences was observed.
Conclusions
One-stage posterior surgery combined with anti-brucella therapy is a practical method in the treatment of LBS with severe neurological compression and spine sagittal imbalance.