bluuebunny/crossref_metadata_2025_split huggingface dataset:
Family socioeconomic status and provincial‐level economic, educational, and health‐related factors as predictors of present‐ and future‐oriented subjective wellbeing in junior high school students in China
Authors: Wu Xiaojing , Gai Xiaosong , Xu Lili , Liu Fangqing , Wang Hong , Kou Hongyang 
Published: February 2022

AbstractIntroductionPrevious studies have examined family socioeconomic status (SES) and regional‐level factors that predict adolescents' present subjective wellbeing (SWB). However, as adolescents' SWB tends to be future‐oriented, this study examined the relationships between family SES and provincial‐level economic, educational, and health‐related factors and adolescents' present‐ and future‐oriented SWB.MethodsThe sample includes 17,341 12‐ to 17‐year‐old adolescents (Mage = 13.86; SDage = 0.79; 9056 girls and 8285 boys) from 31 different provinces of China. Multilevel modeling was used to analyze the data at two levels.ResultsThe findings showed that family SES (Level‐1) was positively correlated with present life satisfaction (LS‐P), present positive affect (PPA), hopeful future expectations (HFE), and positive affect toward future life (FPA), but negatively correlated with present negative affect (PNA) and negative affect toward future life (FNA). Provincial‐level (Level‐2) years of education per capita, average life expectancy, and human development index (HDI) were positively associated with LS‐P, PPA, FPA, and HFE, and negatively associated with PNA; only average life expectancy was negatively associated with FNA. There was no association between gross domestic product (GDP) per capita and SWB. Simple slope analyses demonstrated that, in provinces with relatively less or short years of education per capita, GDP per capita, average life expectancy, or HDI, the correlations between family SES and present‐ and future‐oriented negative affect were stronger.ConclusionsThe present‐ and future‐oriented SWB of adolescents from families with low SES in underdeveloped areas was relatively poor. More psychologically focused education activities are needed for these adolescents.
CircRNA_0008194 functions as a ceRNA to promote invasion of hepatocellular carcinoma via inhibiting miR‐190a/AHNAK signaling pathway
Authors: Liu Wei , Pan Ying , Zhu Hengbo , Zhou Yi , Zhang Hui , Liu Liu , Liu Qing , Ji Guozhong 
Published: February 2022

AbstractBackgroundHepatitis B virus infection was identified as the main risk factor of hepatocellular carcinoma (HCC) in China, which induced a high morbidity and mortality. In recent years, circRNAs were reported involving in the oncogenesis and development of multiple malignant tumors.MethodBioinformatical analysis has been employed to predict the relevant circRNA with AHNAK. The loss of function and gain of function have been used by knocking‐down circRNA through the shRNA technology while overexpressing through lentivirus infection. Dual‐luciferase reporter assay was used to detect circRNA binding to miRNA and target genes. We further used immunoprecipitation technique to detect the binding ability between non‐coding RNAs.ResultsIn this study, according to the previous report, we mainly focused on AHNAK, which has been confirmed as an oncogene involving in the metastasis of HCC. Bioinformatics analysis showed that circ_0008194 could be spliced by AHNAK. In this study, the abnormal upregulated circ_0008194 in tumor tissues was detected. The positive correlation between circ_0008194 and AHNAK was also confirmed. Through knockdown and overexpression of circ_0008194, we conducted in vitro functional studies. We found circ_0008194 could induce the invasion of cells in vitro. Mechanically, circ_0008194 presented the binding ability with miR‐190a causing the suppression of miR‐190a expression, causing the competitive inhibition of AHNAK, resulting in the promotion of EMT.ConclusionOur results suggested that circ_0008194 may act as a sponge to adsorb miR‐190a, thereby promoting the expression of AHNAK and promoting the metastasis of liver cancer tumors.
A method comparison of three immunoassays for detection of neutralizing antibodies against SARS‐CoV‐2 receptor‐binding domain in individuals with adenovirus type‐5‐vectored COVID‐19 vaccination
Authors: Chen Hui , Yu Wanwan , Gao Xiaojiao , Jiang Weijun , Li Xiaojun , Liu Guorui , Yang Yang 
Published: February 2022

AbstractObjectiveDetecting neutralizing antibodies targeting receptor‐binding domain (RBD) is important for the assessment of humoral protection and vaccine efficacy after vaccination. We compared the performance of three surrogate immunoassays for detection of neutralizing antibodies targeting RBD.MethodsWe analyzed 115 serum samples obtained from individuals with Ad5‐vectored COVID‐19 vaccination using two competitive enzyme‐linked immunosorbent assays (Wantai BioPharm and Synthgene Medical Technology) and one competitive chemiluminescence assay (YHLO Biotech). Performance evaluation and methodology comparison were performed according to the Clinical and Laboratory Standards Institute related guidelines.ResultsThe precision met the manufacturers’ statements. The linear range of the WANTAI was 0.0625–0.545 U/ml and the YHLO was 0.260–242.4 U/ml. The WANTAI’s limit of blank (LoB) and limit of detection (LoD) were 0.03 and 0.06 U/ml, respectively. The YHLO’s LoB and LoD were 0.048 and 0.211 U/ml, respectively. The correlations of semi‐quantitative results of Synthgene with quantitative results of YHLO (ρ = 0.566) and WANTAI (ρ = 0.512) were medium. For YHLO and WANTAI, there was a good agreement (0.62) and a strong correlation (ρ = 0.931). Passing–Bablok analysis and Bland‐Altman plot showed a positive bias (112.3%) of the YHLO compared to the WANTAI. The exclusion of samples >50 U/ml did not decrease bias.ConclusionThese findings contribute to a deeper understanding of surrogate viral neutralization assays and provide useful data for future comparison studies.
Dose‐dependent alkaloid sequestration and N‐methylation of decahydroquinoline in poison frogs
Authors: Jeckel Adriana M. , Bolton Sarah K. , Waters Katherine R. , Antoniazzi Marta M. , Jared Carlos , Matsumura Kunihiro , Nishikawa Keisuke , Morimoto Yoshiki , Grant Taran , Saporito Ralph A. 
Published: February 2022

AbstractSequestration of chemical defenses from dietary sources is dependent on the availability of compounds in the environment and the mechanism of sequestration. Previous experiments have shown that sequestration efficiency varies among alkaloids in poison frogs, but little is known about the underlying mechanism. The aim of this study was to quantify the extent to which alkaloid sequestration and modification are dependent on alkaloid availability and/or sequestration mechanism. To do this, we administered different doses of histrionicotoxin (HTX) 235A and decahydroquinoline (DHQ) to captive‐bred Adelphobates galactonotus and measured alkaloid quantity in muscle, kidney, liver, and feces. HTX 235A and DHQ were detected in all organs, whereas only DHQ was present in trace amounts in feces. For both liver and skin, the quantity of alkaloid accumulated increased at higher doses for both alkaloids. Accumulation efficiency in the skin increased at higher doses for HTX 235A but remained constant for DHQ. In contrast, the efficiency of HTX 235A accumulation in the liver was inversely related to dose and a similar, albeit statistically nonsignificant, pattern was observed for DHQ. We identified and quantified the N‐methylation of DHQ in A. galactonotus, which represents a previously unknown example of alkaloid modification in poison frogs. Our study suggests that variation in alkaloid composition among individuals and species can result from differences in sequestration efficiency related to the type and amount of alkaloids available in the environment.
Circulating B10 regulatory cells are decreased in severe and critical COVID-19
Authors: Cervantes-Díaz Rodrigo , Sosa-Hernández Váctor A , Romero-Ramírez Sandra , Torres-Ruiz Jiram , Pérez-Fragoso Alfredo , Meza-Sánchez David E , Gómez-Martín Diana , Maravillas-Montero José L 
Published: February 2022

AbstractThe contribution of B cells in COVID-19 pathogenesis, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Since one of their most relevant functional roles includes their immune-suppressive mechanisms, we decided to evaluate one of the most recognized human B regulatory subpopulations: the IL-10+ B10 cells, during COVID-19 onset. After stimulation of PBMCs for IL-10 induction, we employed multiparametric flow cytometry to determine B10 frequencies in severe and critical COVID-19 patients and then correlated those with clinical and laboratory parameters. Compared with healthy individuals, we detected a significant reduction in the B10 subset in both patient groups, which correlates with some inflammatory parameters that define the disease severity. This evidence suggests an aberrant role of B10 cells in immune responses against SARS-CoV-2 that needs to be further explained.
Cloning and characterization of phosphoglucose isomerase in Lentinula edodes
Authors: Wang Yunxiao , Chen Juhong , Han Jing , Yang Zhengyan , Zhu Jing , Ren Ang , Shi Liang , Yu Hanshou , Zhao Mingwen 
Published: February 2022

AbstractPhosphoglucose isomerase (PGI) is a key enzyme that participates in polysaccharide synthesis, which is responsible for the interconversion of glucose‐6‐phosphate (G‐6‐P) and fructose‐6‐phosphate (F‐6‐P), but there is little research focusing on its role in fungi, especially in higher basidiomycetes. The pgi gene was cloned from Lentinula edodes and named lepgi. Then, the lepgi‐silenced strains were constructed by RNA interference. In this study, we found that lepgi‐silenced strains had significantly less biomass than the wild‐type (WT) strain. Furthermore, the extracellular polysaccharide (EPS) and intracellular polysaccharide (IPS) levels increased 1.5‐ to 3‐fold and 1.5‐fold, respectively, in lepgi‐silenced strains. Moreover, the cell wall integrity in the silenced strains was also altered, which might be due to changes in the compounds and structure of the cell wall. The results showed that compared to WT, silencing lepgi led to a significant decrease of approximately 40% in the β‐1,3‐glucan content, and there was a significant increase of 2‐3‐fold in the chitin content. These findings provide support for studying the biological functions of lepgi in L. edodes.
Para‐aortic lymph node dissection in left‐sided colorectal cancer: Risk factors, prognostic impact, and therapeutic value
Authors: Sun Yanwu , Yu Deng , Zhong Jingming , Lin Yu , Cheng Ningyi , Lin Huiming , Jiang Weizhong , Huang Ying , Chi Pan 
Published: February 2022

AbstractAimThis study aimed to clarify risk factors, prognostic impact, and the therapeutic value of para‐aortic lymph node (PALN) dissection in left‐sided colorectal cancer.MethodOne hundred and fifty‐four patients who underwent primary tumor resection and PALN dissection for left‐sided colorectal cancer were included. Logistic regression analysis was used to identify risk factors for PALN metastasis. Cox regression analysis was performed to identify risk factors for overall survival (OS).ResultsPALN metastasis was pathologically confirmed in 47 patients (30.5%). Postoperative complications occurred in 42 patients (27.3%). Pathological N stage (OR = 4.661, p = 0.034) and inferior mesenteric artery LNs metastasis (OR = 6.048, p = 0.003) remained to be independently associated with PALN metastasis, the 5‐year OS rate and median survival in patients with PALN metastasis was 37.7% and 24 months. Elevated preoperative serum CA19‐9 level (HR = 1.006, p = 0.007), number of positive LNs > 7 (HR = 7.263, p = 0.001), and mucinous adenocarcinoma or signet ring cell carcinoma (HR = 6.511, p = 0.001) were independently associated with OS in patients with PALN metastasis.ConclusionPALN dissection in addition to primary tumor resection have acceptable postoperative complications and may be oncologically beneficial in selected left‐sided colorectal cancer patients with clinically suspicious PALN metastasis.
Evidence‐based posttraumatic stress disorder treatment in a community sample: Military‐affiliated versus civilian patient outcomes
Authors: Jacoby Vanessa M. , Straud Casey L. , Bagley Jenna M. , Tyler Hannah , Baker Shelby N. , Denejkina Anna , Sippel Lauren M. , Kaya Robert , Rozek David C. , Fina Brooke A. , Dondanville Katherine A. ,   for the STRONG STAR Training Initiative
Published: February 2022

AbstractPosttraumatic stress disorder (PTSD) is a significant mental health issue among military service members and veterans. Although the U.S. Department of Veterans Affairs (VA) provides crucial resources for behavioral health care, many veterans seek mental health services through community clinics. Previous research illustrates that military and veteran patients benefit less from evidence‐based treatments (EBTs) for PTSD than civilians. However, most PTSD treatment outcome research on military and veteran populations is conducted in VA or military settings. Little is known about outcomes among military‐affiliated patients in community settings. The primary aim of this study was to directly compare civilian versus military‐affiliated patient outcomes on PTSD and depression symptoms using the PTSD Checklist for DSM‐5 (PCL‐5) and the nine‐item Patient Health Questionnaire (PHQ‐9) in a community setting. Participants (N = 502) included military‐affiliated (veteran, Guard/Reservist, active duty) and civilian patients who engaged in cognitive processing therapy (CPT) or prolonged exposure (PE) for PTSD in community clinics. Both groups demonstrated significant reductions on the PCL‐5, military‐affiliated: d = −0.91, civilian: d = ‐1.18; and PHQ‐9, military‐affiliated: d = ‐0.65, civilian: d = ‐0.88, following treatment. However, military‐affiliated patients demonstrated smaller posttreatment reductions on the PCL‐5, Mdiff = 5.75, p = .003, and PHQ‐9, Mdiff = 1.71, p = .011, compared to civilians. Results demonstrate that military‐affiliated patients benefit from EBTs for PTSD, albeit to a lesser degree than civilians, even in community settings. These findings also highlight the importance of future research on improving EBTs for military personnel with PTSD.
Remote silicate supply regulates spring phytoplankton bloom magnitude in the Gulf of Maine
Authors: Zang Zhengchen , Ji Rubao , Liu Yonggang , Chen Changsheng , Li Yun , Li Siqi , Davis Cabell S. 
Published: February 2022

AbstractSpring phytoplankton blooms in the Gulf of Maine (GoM) are sensitive to climate‐related local and remote forcing. Nutrient supply through the slope water intrusion has been viewed as critical in regulating the GoM spring blooms, with an assumption that nitrogen is the primary limiting nutrient. In recent years, this paradigm has been challenged, with silicate being recognized as another potential limiting nutrient, but the source of silicate and its associated water mass remain difficult to be determined. In this study, a time series of spring bloom magnitude was constructed using a self‐organizing map algorithm, and then correlated with the fluctuation of water composition in the deep Northeast Channel. The results reveal the importance of silicate supply from previously less‐recognized deep Scotian Shelf Water inflow. This study offers a new hypothesis for spring bloom regulation, providing a better understanding of mechanisms controlling the spring bloom magnitude in the GoM.
Analysis of one‐dimensional nonlinear rheological consolidation of saturated clay considering self‐weight stress and Swartzendruber's flow rule
Authors: Cui Penglu , Cao Wengui , Xu Zan , Wei Yunbo , Li Huixin 
Published: February 2022

AbstractConsidering the nonlinear rheological properties of saturated clay, the modified unified hardening (UH) constitutive relation was introduced to describe the nonlinear rheological deformation. Meanwhile, Swartzendruber's flow law was used to simulate the non‐Darcian flow of pore water in the progress of rheological consolidation. Consequently, a nonlinear rheological consolidation model (NRCM), for one‐dimensional consolidation, that simultaneously incorporates nonlinear stress‐strain‐time relationship, Swartzendruber's flow, self‐weight stress, and variable permeability coefficient has been developed here. The finite volume method (FVM) was utilized to solve the NRCM system, and its effectiveness was verified by comparing with the existing consolidation data. The parametric analysis results indicate that the soil's nonlinear rheological effect induced the excess pore water pressure (EPWP)’s accumulation in the initial loading. This phenomenon became more significant when considering the self‐weight stress or as the initial overconsolidated parameter, soil thickness, and seepage parameter increased. In contrast, it weakened with the increase of external loading. Additionally, considering the self‐weight stress slowed down the whole dissipation of EPWP in the initial period of loading. Nevertheless, it accelerated the nonlinear consolidation process in the middle and late loading stages.
Effects of grain size, solution salinity and pH on the electrical response of oil‐bearing carbonate sands
Authors: Deng Yaping , Qian Jiazhong , Luo Qiankun , Ma Haichun , Ma Lei , Xu Kaijian 
Published: February 2022

ABSTRACTResearchers around the world have carried out numerous studies on the electrical response of oil‐contaminated silica sands. However, few studies consider the oil contamination in carbonate sands that exhibit quite different characteristics to silica sands. The purpose of this work is to study the influence of grain size (0.5–1.0, 1–3 and 3–5 mm), solution salinity (100, 10 and 1 Ω·m) and pH (4.58, 6.5 and 9.25) on the resistivity and saturation exponent of oil‐bearing carbonate sands. Experimental columns were packed with crushed limestone grains, solution and oil. Electrical resistivity measurements were subsequently performed on the packed columns. Grain size exhibits a complex effect on the electrical response of partially saturated limestone grains. Resistivity decreases slightly with increasing grain size when water saturation is greater than 0.4, while it shows an inconsistent trend when water saturation is lower than 0.4. The resulting saturation exponent n decreases slightly when grain size increases from 0.5–1 mm to 1–3 mm, while it shows a positive change in n values for grain size of 3–5 mm. Changes in solution salinity significantly influence the resistivity and saturation exponent of limestone grains, with higher solution salinity resulting in lower resistivity and higher saturation exponent. However, with solution pH increasing from 4.58 to 9.25, it shows very limited effect on the resistivity and saturation exponent of limestone grains. This can be attributed to the strong buffering ability of limestone. Effects of grain size, solution salinity and pH on the resistivity and saturation exponent of oil‐bearing carbonate sands were investigated. Resistivity is found to show a decreasing trend with increasing grain size from 0.5–1 mm to 3–5 mm above a certain saturation (e.g. Sw > 0.4), while it shows a rapid increase below a certain saturation (e.g., Sw0.4) for a larger grain size of 3–5 mm. Resistivity decreases and the saturation exponent increases with the increase of solution salinity for oil‐bearing carbonate sands. Resistivity and saturation exponent do not vary significantly with solution pH ranging from 4.58 to 9.25 for oil‐bearing carbonate sands.
A singularity‐free online neural network‐based sliding mode control of the fixed‐wing unmanned aerial vehicle optimal perching maneuver
Authors: Song Yansui , Tang Yong , Ma Bo , Xu Bin 
Published: February 2022

AbstractThe perching behavior of birds is very common in nature, but it is extraordinary for fixed‐wing unmanned aerial vehicles (UAVs), because of post‐stall maneuver. The main problems of the perching maneuver are weak control authority and strong model nonlinearity. This paper proposed a control method to realize the fixed‐wing perch maneuver. For the problem of weak control authority, an optimal trajectory is designed. And a feasible reference trajectory is given under the conditions of model dynamic characteristics and actuator constraints. For the problem of strong nonlinearity, a singularity‐free online neural network‐based sliding mode controller is designed. The simulation test shows that the proposed controller can ensure a reliable perching maneuver under the initial state deviation.
Pairwise joint modeling of clustered and high‐dimensional outcomes with covariate missingness in pediatric pneumonia care
Authors: Gachau Susan , Njagi Edmund Njeru , Molenberghs Geert , Owuor Nelson , Sarguta Rachel , English Mike , Ayieko Philip 
Published: February 2022

AbstractMultiple outcomes reflecting different aspects of routine care are a common phenomenon in health care research. A common approach of handling such outcomes is multiple univariate analyses, an approach which does not allow for answering research questions pertaining to joint inference. In this study, we sought to study associations among nine pediatric pneumonia care outcomes spanning assessment, diagnosis and treatment domains of care, while circumventing the computational challenge posed by their clustered and high‐dimensional nature and incompletely recorded covariates. We analyzed data from a cluster randomized trial conducted in 12 Kenyan hospitals. There were varying degrees of missingness in the covariates of interest, and these were multiply imputed using latent normal joint modeling. We used the pairwise joint modeling strategy to fit a correlated random effects joint model for the nine outcomes. This entailed fitting 36 bivariate generalized linear mixed models and deriving inference for the joint model using pseudo‐likelihood theory. We also analyzed the nine outcomes separately before and after multiple imputation. We observed joint effects of patient‐, clinician‐ and hospital‐level factors on pneumonia care indicators before and after multiple imputation of missing covariates. In both pairwise joint modeling and separate univariate analysis methods, enhanced audit and feedback improved documentation and adherence to recommended clinical guidelines over time in six and five pneumonia care indicators, respectively. Additionally, multiple imputation improved precision of parameter estimates compared to complete case analysis. The strength and direction of association among pneumonia outcomes varied within and across the three domains of pneumonia care
Young‐type interference in soft lepton scattering of diatomic homonuclear molecules
Authors: Barp Marcos V. , Arretche Felipe 
Published: February 2022

AbstractInterference patterns in the scattering of positrons and electrons by diatomic homonuclear molecules are ab initio calculated. Our results are compared to model potential calculations with incident particles in twisted and plane wave states. All calculations are obtained in the first Born approximation framework. The comparison of the elastic differential cross sections shows how an ab initio description of the electronic molecular structure influence the interference minima structure. The origin of such patterns is also discussed.