bluuebunny/crossref_metadata_2025_split huggingface dataset:
P. ae induced necroptosis signaling and NLRP3 inflammasome cross-talking in epithelium facilitates acute lung injury
Authors: Li Haoyang , Guan Jieying , Chen Jiaqian , Sun Weimin , He Andong , Chen Honglv , Wen Yuhuan , Chen Qile , Xie Shiyun , Tao Ailin , Yan Jie 
Published: April 2022

Abstract
Pseudomonas aeruginosa induced acute lung injury is such a serious risk to public health throughout the world, but the pathological regulation remains unclear. Here, we reported that P. ae mediated epithelial necroptosis leads to subsequent immune responses and plays an important role in pulmonary pathological process. Pharmacological and genomic ablation of necroptosis signaling ameliorate P. ae mediated acute lung injury and pulmonary inflammation. Our results further proved NLRP3 inflammasome to involve in P. ae induced ALI. Mechanism investigation revealed the cross-talking patterns between inflammasome activation and necroptosis signaling pathway that MLKL-dependent necroptosis signaling promotes the change of mitochondrial membrane potential for the release of reactive oxygen species (ROS), which is the important trigger for functional inflammasome activation. Furthermore, antioxidants such as Mito-TEMPO was confirmed to significantly restrain inflammasome activation in epithelial cells, resulting in a reduction in P. ae induced ALI and pulmonary inflammation. Taken together, our findings revealed that necroptosis-triggered NLRP3 inflammasome in epithelium plays a key role in P. ae mediated injury, which could be a potential therapeutic target for acute lung injury.
Methanol fixed feeder layers altered the pluripotency and metabolism of bovine pluripotent stem cells
Authors: Xu Wenqiang , Hao Ruifeng , Wang Jing , Gao Lingna , Han Xuejie , Li Chen , Zhang Hui , Li Xueling 
Published: April 2022

Abstract
The pluripotency maintenance of pluripotent stem cells (PSCs) cultured in vitro requires the suitable microenvironment, which is commonly provided by the feeder layer. However, the preparation of feeder layer is time consuming and labor exhaustive. More importantly, the feeder cells treated with mitomycin or gamma-ray irradiation brings heterologous contamination to stem cells. The feeder-free PSC cultures are associated with high costs because of the requirement for additional supplements and special media. In this study, we characterize the pluripotency and metabolic status of bovine ESCs-F7 (classic bESCs lines, abbreviated as F7), which were cultured on methanol fixed mouse embryonic fibroblasts (MT-MEFs) or mitomycin C treated MEFs (MC-MEFs). MT-MEFs could be reused several times and were highly resistant to digestive enzymes. The relative expression levels of pluripotenct markers were different between F7 cultured on MT-MEFs (marked as MT-F7) and those cultured on the MC-MEFs (MC-F7). The long-term cultured MT-F7 cells formed embryoid bodies in vitro, showing the ability to differentiate into endodermal, ectodermal, and mesodermal germ layers like MC-F7. RNA-sequencing analysis showed that the replacement of the feeder layer from MC-MEFs to MT-MEFs lead to a novel steady transition of the F7, which included alteration of the expression patterns of genes that regulate pluripotency and metabolism. Further, the long-term cultured bovine expanded pluripotent stem cells (bEPSCs) on MT-MEFs (MT-bEPSCs) formed classical colonies, maintained pluripotency, and demonstrated elevated level of metabolic activity. In conclusion, this study demonstrated that methanol-fixed MEFs were efficient feeder layer that maintain the unique pluripotency and distinctive metabolic characteristics of the bPSCs cultured in vitro.
Assessment of the thermal degradation kinetics of fresh green coconut husk (Cocus nucifera L.) powder and after methyl levulinate production
Authors: Silva Poliana Pinheiro da , Silva Leonete Cristina de Araújo Ferreira Medeiros , Neto Eduardo Lins de Barros , Nascimento Paula Fabiane Pinheiro do , Melo Ricardo Paulo Fonseca , Lopes Francisco Wendell Bezerra , Duarte Lindemberg de Jesus Nogueira 
Published: April 2022

Abstract
This study describes the recycling of green coconut husk powder (CHP) aimed at the sustainable production of methyl levulinate. The preliminary step involved thermogravimetric analysis of green (CHP) and residues after synthesis of methyl levulinate with the catalysts aluminum sulfate (CHP-AS) and titanium dioxide (CHP-TD), based on a compositional analysis, kinetic degradation and determination of activation energy using the Ozawa-Flynn-Wall (OWF) method. Thermogravimetric analyses showed significant differences between the main constituents of CHP, CHP-AS and CHP-TD residues: hemicellulose (18.18, 9.78 and 12.17%), cellulose (44.65, 26.18 and 44.23%) and lignin (20.09, 19.89 and 19.58%), respectively. The methyl levulinate concentration obtained by the reaction between CHP and aluminum sulfate was 16.53 g.L− 1, due to the participation of hemicellulose and cellulose. The results showed that the activation energies calculated using the OFW method were 142 kJ.mol− 1 (CHP), 125 kJ.mol− 1 (CHP-AS) and 180 kJ.mol− 1 (CHP-TD).
Non–Polynomial Quintic Spline Method for solving the generalized regularized long wave (GRLW) equation
Authors: Hammad D. A. , Semary Mourad S. , Khattab Ahmed G. 
Published: April 2022

Abstract
In this paper, a new technique for solving the generalized regularized long wave (GRLW) equation is investigated. This technique depends on a combination of the non–polynomial quintic spline method (NPQSM) with the finite difference approximation and Crank–Nicolson scheme. The stability analysis of the present method is concluded using the Von–Neumann method. Also, the most popular three invariants of motion of GRLW equation are obtained for the given problems and their numerical results are compared with the analytical values. In addition, a comparison between the present method and the previous methods and the error norms L2 and L∞ are presented to show the accuracy of the suggested method.
Electron liquid state in the spin-1/2 anisotropic Kondo lattice
Authors: Karnaukhov Igor N. 
Published: April 2022

Abstract
In the framework of the mean field approach, we provide analytical and numerical solution of the spin- 1/2 anisotropic Kondo lattice for arbitrary dimension at half filling. Nontrivial solution for the amplitude of the field opens a gap in the fermion spectrum of an electron liquid in which local moments on the lattice sites are realized. The ground state in the insulator state is determined by a static Z2 field of local moments, which forms the lattice with a double cell, conduction electrons move in this field. Due to hybridization between electron states a large Fermi surface is formed in the Kondo lattice. A gap in the quasi-particle spectrum is calculated depending on the magnitudes of exchange integrals for the simple lattices with different dimension. The proposed approach is also valid for describing the Kondo lattice with weak anisotropy of the exchange interaction, which makes it possible to study the behavior of the spin- 1/2 Kondo lattice with an isotropic exchange interaction
The impact of teat and bottle design on nipple confusion: a double-blind randomized controlled trial
Authors: Chanprapaph Pharuhas , Sutchritpongsa Sureelak , Rojmahamongkol Pat , Chuchoang Siripan , Arthan Jenjira , Komoltri Chulaluk 
Published: April 2022

Abstract
Background: Nipple confusion has been reported in some infants who receive both breastfeeding and bottle feeding at early stage. Two types of nipple confusion including breast refusal (type A) and bottle refusal (type B) are described. Breast refusal may decrease maternal breast stimulation and result in low breast milk production while bottle refusal could create stress and prevent mothers from resuming their normal lives. This study aimed to explore whether the design of teat and bottle could influence the nipple confusion incidence, infant growth, feeding type and infant behaviors.Method: A double-blind randomized controlled trial study has been conducted during June 2016 and September 2019. Forty normal eligible exclusively breastfed newborn-mother dyads with given informed consent were prospectively enrolled for 8 weeks of mixed feeding due to maternal return to work or study. They were randomly allocated into group A (Pigeon, Peristaltic PlusTM nipple, SS size) and group B (Philips, Avent NaturalTM Slow Flow nipple, SS size). All data including feeding type, milk intake, nipple confusion rate and infant behavior were collected for statistical analysis.Result: Between 21 pairs of group A and 19 pairs of group B, nipple confusion was increasing from 22.5% to 50% during the study. Initially, bottle refusal was found 22.5% while breast refusal was more detected (32.5%) lately. Infant growth and development including weight, length, and head circumference were comparable in both groups. Infants in group A seemed to have lower breast refusal rate than infants in group B (28.6% vs 36.8%). Nevertheless, the bottle refusal rate was rather higher in group A than group B (23.8% vs 10.5%). The earlier the mixed feeding (< 5 weeks), the higher the nipple confusion rate (15% vs 7.5% at 4 weeks; 15% vs 35% at 12 weeks). Infant aerophagia, crying, abnormal latch-on and milk aspiration could be observed in both groups with no statistical difference while constipation was only observed in group B (5.3-15.8 %).Conclusion: Nipple confusion could be found in exclusive breastfed infants after switching to mixed feeding. In Phillips’ group, nipple confusion rate was about 2 times higher than Pigeon’s group at 4 weeks, then, the rate was increasing to approximately 50% in both groups by 12 weeks. Aerophagia was more observed in Pigeon’s group while constipation was commonly found in Philips’ group. Early infant mixed feeding before 5 weeks could cause the higher nipple confusion rate and should be discouraged. As each product has both pros and cons, it has to be thoughtful to choose the proper design to achieve the infant’s need.
The antiviral action of resveratrol against Zika virus through the regulation of interferon responses by HMGB1
Authors: Chin Kim-Ling , Zainal Nurhafiza , Sam Sing-Sin , AbuBakar Sazaly 
Published: April 2022

Abstract
Zika virus (ZIKV) infection is a public health concern and currently there is no specific therapeutic or approved vaccine. Resveratrol (RESV), a natural antiviral compound, has been shown to possess antiviral properties, but the mechanisms of action against ZIKV remain unknown. This study aimed to investigate the role of the high mobility group box 1 protein (HMGB1) in the underlying anti-ZIKV mechanisms of RESV. HMGB1 protein expression and ZIKV replication in both RESV-treated wild-type (WT) and HMGB1-knockdown (shHMGB1) Huh7 cells were analyzed using ELISA, immunofluorescence assay, immunoblot assay, focus-forming assay and qRT-PCR. HMGB1’s role was explored by evaluating the changes in the type-1 interferon (IFN) response genes using qRT-PCR and immunoblot assays. The treatment of ZIKV-infected WT Huh7 cells with RESV significantly reduced ZIKV titers by >90% (P < 0.001) and inhibited ZIKV-induced HMGB1 translocation (P < 0.001), resulting in nuclear HMGB1 accumulation. Compared to WT Huh7 cells, shHMGB1 Huh7 cells without RESV treatment showed a significant increase in the infectious virus titers and RNA with a maximum rise of 74% (P < 0.001) and 65% (P < 0.01), respectively. RESV treatment of ZIKV-infected WT Huh7 cells significantly increased the MxA (one of the classical interferon-stimulated genes, ISGs) and IFN-β levels (p < 0.05). The treatment of infected shHMGB1 Huh7 cells with RESV showed a less effective antiviral response (P > 0.05) and did not cause changes in the expressions of MxA and IFN-β. Findings presented here suggest that RESV possesses therapeutic activity against ZIKV infection and the mechanism of action is mainly attributed to HMGB1 nuclear retention, which could upregulate the type-1 IFN and ISGs.
A Study of Dose Painting Guided by Three-Dimensional Arterial Spin Labeling for Non-Enhancing Low-Grade Gliomas During Radiotherapy
Authors: Zhu Zihong , Gong Guanzhong , Wang Lizhen , Su Ya , Lu Jie , Yin Yong 
Published: April 2022

Abstract
Background: The purpose of this study was to investigate the feasibility and dosimetric characteristics of dose painting for non-enhancing low-grade gliomas (NE-LGGs) guided by three-dimensional arterial spin labeling (3D-ASL). Methods: Eighteen patients with NE-LGGs were enrolled. 3D-ASL, T2 fluid-attenuated inversion recovery (T2 Flair) and contrast-enhanced T1-weighted magnetic resonance images were obtained. The gross tumor volume (GTV) was delineated on the T2 Flair. The hyper-perfusion region of the GTV (GTV-ASL) was determined by 3D-ASL, and the GTV-SUB was obtained by subtracting the GTV-ASL from the GTV. The clinical target volume (CTV) was created by isotropically expanding the GTV by 1cm. The planning target volume (PTV), PTV-ASL were obtained by expanding the external margins of the CTV, GTV-ASL, respectively. PTV-SUB was generated by subtracting PTV-ASL from PTV. Three plans were generated for each patient: a conventional plan (plan1) without dose escalation delivering 95%-110% of 45-60Gy in 1.8–2Gy fractions to the PTV and two dose-painted plans (plan2 and plan3) with dose escalating by 10%-20% (range, 50-72Gy) to the PTV-ASL based on plan1. The plan3 was obtained from plan2 without the maximum dose constraint. The dosimetric differences among the three plans were compared. Results: The volume ratio of the PTV-ASL to the PTV was (23.49±11.94) % (Z=-3.724, P=0.000). Compared with plan 1, D2%, D98% and Dmean of PTV-ASL increased by 14.67%,16.17% and 14.31% in plan2 and 19.84%,15.52% and14.27% in plan3, respectively (P<0.05); the D2% of the PTV, PTV-SUB increased by 11.89% and 8.34% in plan2, 15.89% and 8.49% in plan3, respectively (P<0.05). The PTV coverages were comparable among the three plans (P>0.05). In plan2 and plan3, the conformity indexes decreased by 18.60% and 12.79%; while the homogeneity index increased by 1.43 and 2 times (P<0.05). Compared with plan1, the D0.1cc of brain stem and Dmax of optic chiasma were slightly increased in plan 2 and plan 3, and the absolute doses met the dose constraint. The doses of the other organs at risk (OARs) were similar among the three plans (P>0.05). Conclusions: The dose delivered to hyper-perfusion region derived from 3D-ASL can increased by 10-20% while respecting the constraints to the OAR, which provides a basis for future individualized and precise radiotherapy for NE-LGGs.
Sources and Biogeochemical Processing of Dissolved Organic Matter in the Middle Reach of the Yangtze River: Insights from Optical Properties and Nitrate Dual-Isotopes
Authors: Liao Jin , Huang Qinghui , Liu Guangning , Wang Ningtao , Qiu Zhipu , Hu Chaoyong , Spencer Robert G.M. 
Published: April 2022

Abstract
Understanding the sources and biogeochemical processing of dissolved organic matter (DOM) and nutrients within large rivers is vital to carbon cycling and water management. However, there remains great uncertainty regarding aquatic DOM composition and source proportioning resulting from spatial-temporal heterogeneity. In this study, multiple techniques were applied to track riverine DOM and nutrients over different flow regimes along the middle reach of the Yangtze River. Combined with nitrate dual isotopes, three different organic sources have been identified including terrestrial humic substances, “non-natural” terrestrial humic substances associated with the input of urban sewage, and tyrosine-like substances related to microbiological activities. The higher aromatic degree of DOM, higher \({{\delta }}^{18}{\text{O}}_{{\text{N}\text{O}}_{3}}\), and lighter \({{\delta }}^{15}{\text{N}}_{{\text{N}\text{O}}_{3}}\) signals in the rainy season suggested that soil erosion by intensive monsoon rainfall was the main factor for the release of carbon and nitrogen. During the dry season, nitrate from soil solution in the riparian zone with denitrification and inputs of urban sewage might be responsible for the enrichment of \({{\delta }}^{15}{\text{N}}_{{\text{N}\text{O}}_{3}}\) and higher tyrosine-like substances. At the spatial scale, the same terrestrial DOM was observed between the distal surface water in the Three Gorge Reservoir and the outlet, which was mainly caused by the density currents inducing water intrusion in the reservoir. An ever increasing proportion of tyrosine-like substances longitudinally indicated the cumulative intensity of human activities was becoming stronger along the mainstream. The study advances our evaluation of different carbon and nitrogen in sources in the Yangtze River and provides insights into the management of large monsoonal rivers.
Tracing the dispersal route of the invasive Japanese beetle Popillia japonica
Authors: Strangi Agostino , Paoli Francesco , Nardi Francesco , Shimizu Ken , Kimoto Troy , Iovinella Immacolata , Bosio Giovanni , Roversi Pio Federico , Carapelli Antonio , Marianelli Leonardo 
Published: April 2022

Abstract
The Japanese beetle, Popillia japonica, is a highly polyphagous Scarabeidae native to Japan that colonized North America and Azores in the last century and has recently invaded Italy and Switzerland. Considering its economic impact to the horticulture and turfgrass industries, this species was ranked within the EU priority pests list in 2019. In order to reconstruct the source of introductions of this pest, we investigated the genetic variability of P. japonica in its native and invaded areas worldwide by analyzing 9 microsatellite loci and two mitochondrial genes, COI and CytB. There are two populations of Japanese beetles, from a limited area within central Japan, that are likely the source of the North American population. Moreover, the microsatellite data and resulting phylogeographic reconstruction suggests that the two European populations originated from independent introductions from southeast (Azores) and northeastern (Italy and Switzerland) North America (1). (1) For simplicity, in this paper North America refers to Canada and USA
Layered Methods for Updating AI-compatible TCAMS in 5G/6G WSNs
Authors: Abbasi Mahdi , Vakilian Shobeir , Vakilian Shakoor , Khosravi Mohammad. R , Abdoli Hatam 
Published: April 2022

Abstract
Classification is a fundamental processing task in advanced network systems. This technique is exploited in 5G/6G wireless sensors networks where flow-based processing of the internet packets is highly demanded by intelligent applications that analyze big volumes of data in a limited time. In this process, the input packets are classified into specific streams by matching to a set of filters. TCAM memory is used in hardware implementation of internet packets. However, due to the parallel search capabilities, this memory leads to an increase in the speed and drop of hardware bundles compared to other types of software bundles, but with the increase in the number of rules stored in its layers, the power required for searching, inserting and eliminating increases. Various architectures have been proposed to solve this problem, but none of them has proposed a plan to reduce power consumption while updating the rules in the TCAM memory. In this paper, two algorithms are presented for reducing power consumption during TCAM memory upgrades. The key idea in the proposed algorithms is the reduction in the search range as well as the number of displacements while inserting and deleting rules in TCAM. Implementation and evaluation of proposed methods represent a reduction of more than 50% of the number of visits to TCAM in both proposed algorithms, as well as reducing the update time in the second proposed algorithm compared to the first proposed algorithm which confirms the efficiency of both methods.
Biosensing firefly luciferin synthesis in bacteria reveals a cysteine-dependent quinone detoxification route in Coleoptera
Authors: de Souza Daniel Rangel , Silva Jaqueline R da , Moreira Ariele , Viviani Vadim R. 
Published: April 2022

Abstract
The evolutive origin of luciferin in bioluminescent beetles remains a mystery. Luciferin has cysteine and benzoquinone as precursors, and it can be spontaneously produced from these compounds under neutral buffer conditions. However, its biosynthetic pathway and alternative functions remain largely unveiled. We have set up a cellular bioluminescent sensing method for luciferin synthesis from cysteine and benzoquinone using E. coli and Pichia pastoris expressing the bright Amydetes vivianii firefly luciferase and P.termitilluminans click beetle luciferase. In the presence of D-cysteine and benzoquinone, intense bioluminescence is quickly by transformed E.coli, indicating the expected formation of D-luciferin. Starting with L-cysteine and benzoquinone, the bioluminescence is weaker and delayed in comparison with experiments starting with D-cysteine, indicating that bacteria expressing firefly luciferase first produce L-luciferin, and then racemize it to D-luciferin in the presence of endogenous bacterial esterases, CoA and firefly luciferase. Whereas p-benzoquinone is extremely toxic to bacteria (IC50 ~25 µM), in the presence of cysteine this toxicity is considerably reduced, maintaining cell viability even in higher concentrations of p-benzoquinone (3.6 mM). Transcriptional analysis of photogenic and non-photogenic tissues of fireflies, showed the presence of several gene products involved with the sclerotization/tanning pathways in the photogenic tissues, with the exception of enzymes involved with the last steps of these pathways, suggesting the possible accumulation of quinone intermediates in lanterns. These results and the high abundance of cysteine producing enzymes suggest that luciferin arose as a detoxification byproduct of the reaction of accumulated toxic quinone intermediates with cysteine during the sclerotization/tanning in Coleoptera.
Construction of a new automatic grading system for jaw bone mineral density level based on deep learning using cone beam computed tomography
Authors: Xiao Yanjun , Liang Qihui , Zhou Lin , He Xuezhi , Lv Lingfeng , Chen Jiang , Endian Su , Jianbin Guo , Wu Dong , Lin Lin 
Published: April 2022

Abstract
Objective To develop and verify an automatic classification method using artificial intelligence deep learning to determine the bone mineral density level of the implant site in oral implant surgery from radiographic data obtained from cone beam computed tomography (CBCT) images.Materials and Methods Seventy patients with mandibular dentition defects were scanned using CBCT. These Digital Imaging and Communications in Medicine (DICOM) data were cut into 605 training sets, and then the data were processed with data standardization, and the Hounsfiled Unit (HU) value level was determined as follows: Type 1, 1000–2000; type 2, 700–1000; type 3, 400–700; type 4, 100–400; and type 5, −200–100. Four trained dental implant physicians manually identified and classified the area of the jaw bone density level in the image using the software LabelMe. Then, with the assistance of the HU value generated by LabelMe, a physician with 20 years of clinical experience confirmed the labeling level. Finally, the HU mean values of various categories marked by dental implant physicians were compared to the mean values detected by the artificial intelligence model to assess the accuracy of artificial intelligence classification.Results After the model was trained on 605 training sets, the statistical results of the HU mean values of various categories in the dataset detected by the model were almost the same as the HU grading interval on the data annotation.Conclusion This new classification provides a more detailed solution to guide surgeons to adjust the drilling rate and tool selection during preoperative decision-making and intraoperative hole preparation for oral implant surgery.