bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic effect of plasma-activated medium and novel indirubin derivatives on human skin cancer cells by activation of the AhR pathway
Authors: Rebl Henrike , Sawade Marie , Hein Martin , Bergemann Claudia , Wende Manuela , Lalk Michael , Langer Peter , Emmert Steffen , Nebe Barbara 
Published: February 2022

AbstractDue to the increasing number of human skin cancers and the limited effectiveness of therapies, research into innovative therapeutic approaches is of enormous clinical interest. In recent years, the use of cold atmospheric pressure plasma has become increasingly important as anti-cancer therapy. The combination of plasma with small molecules offers the potential of an effective, tumour-specific, targeted therapy. The synthesised glycosylated and non glycosylated thia-analogous indirubin derivatives KD87 and KD88, respectively, were first to be investigated for their pharmaceutical efficacy in comparison with Indirubin-3'-monoxime (I3M) on human melanoma (A375) and squamous cell carcinoma (A431) cells. In combinatorial studies with plasma-activated medium (PAM) and KD87 we determined significantly decreased cell viability and cell adhesion. Cell cycle analyses revealed a marked G2/M arrest by PAM and a clear apoptotic effect by the glycosylated indirubin derivative KD87 in both cell lines and thus a synergistic anti-cancer effect. I3M had a pro-apoptotic effect only in A431 cells, so we hypothesize a different mode of action of the indirubin derivatives in the two skin cancer cells, possibly due to a different level of the aryl hydrocarbon receptor and an activation of this pathway by nuclear translocation of this receptor and subsequent activation of gene expression.
Development of transgenic Daphnia magna for visualizing homology-directed repair of DNA
Authors: Fatimah Rizky Mutiara , Adhitama Nikko , Kato Yasuhiko , Watanabe Hajime 
Published: February 2022

AbstractIn the crustacean Daphnia magna, studying homology-directed repair (HDR) is important to understand genome maintenance during parthenogenesis, effects of environmental toxicants on the genome, and improvement of HDR-mediated genome editing. Here we developed a transgenic D. magna that expresses green fluorescence protein (GFP) upon HDR occurrence. We utilized the previously established reporter plasmid named DR-GFP that has a mutated eGFP gene (SceGFP) and the tandemly located donor GFP gene fragment (iGFP). Upon double-strand break (DSB) introduction on SceGFP, the iGFP gene fragment acts as the HDR template and restores functional eGFP expression. We customized this reporter plasmid to allow bicistronic expression of the mCherry gene under the control of the D. magna EF1α-1 promoter/enhancer. By CRISPR/Cas-mediated knock-in of this plasmid via non-homologous joining, we generated the transgenic D. magna that expresses mCherry ubiquitously, suggesting that the DR-GFP reporter gene is expressed in most cells. Introducing DSB on the SceGFP resulted in eGFP expression and this HDR event could be detected by fluorescence, genomic PCR, and quantitative reverse-transcription PCR, suggesting this line could be used for evaluating HDR. The established reporter line might expand our understanding of the HDR mechanism and also improve the HDR-based gene-editing system in this species.
Intrinsic signal optoretinography of dark adaptation kinetics
Authors: Kim Tae-Hoon , Ding Jie , Yao Xincheng 
Published: February 2022

AbstractDelayed dark adaptation due to impaired rod photoreceptor homeostasis has been reported as the earliest symptom of eye diseases such as age-related macular degeneration, diabetic retinopathy, and retinitis pigmentosa. Objective measurement of dark adaptation can facilitate early diagnosis to enable prompt intervention to prevent vision loss. However, there is a lack of noninvasive methods capable of spatiotemporal monitoring of photoreceptor changes during dark adaptation. Here we demonstrate functional optical coherence tomography (OCT) for in vivo intrinsic signal optoretinography (ORG) of dark adaptation kinetics in the C57BL/6J mouse retina. Functional OCT revealed a shortening of the outer retina, a rearrangement of the cone and rod photoreceptor interdigitation zone, and a reduction in intrinsic signal amplitude at the photoreceptor inner segment ellipsoid (ISe). A strong positive correlation between the outer retinal shortening and ISe intensity reduction was also confirmed. Functional OCT of dark adaptation kinetics promises an objective method for rapid ORG assessment of physiological integrity of retinal photoreceptors.
Developing a lifestyle intervention program for overweight or obese preconception, pregnant and postpartum women using qualitative methods
Authors: Ku Chee Wai , Leow Shu Hui , Ong Lay See , Erwin Christina , Ong Isabella , Ng Xiang Wen , Tan Jacinth J. X. , Yap Fabian , Chan Jerry Kok Yen , Loy See Ling 
Published: February 2022

AbstractThe time period before, during and after pregnancy represents a unique opportunity for interventions to cultivate sustained healthy lifestyle behaviors to improve the metabolic health of mothers and their offspring. However, the success of a lifestyle intervention is dependent on uptake and continued compliance. To identify enablers and barriers towards engagement with a lifestyle intervention, thematic analysis of 15 in-depth interviews with overweight or obese women in the preconception, pregnancy or postpartum periods was undertaken, using the integrated-Promoting Action on Research Implementation in Health Services framework as a guide to systematically chart factors influencing adoption of a novel lifestyle intervention. Barrier factors include time constraints, poor baseline knowledge, family culture, food accessibility, and lack of relevant data sources. Enabling factors were motivation to be healthy for themselves and their offspring, family and social support, a holistic delivery platform providing desired information delivered at appropriate times, regular feedback, goal setting, and nudges. From the findings of this study, we propose components of an idealized lifestyle intervention including (i) taking a holistic life-course approach to education, (ii) using mobile health platforms to reduce barriers, provide personalized feedback and promote goal-setting, and (iii) health nudges to cultivate sustained lifestyle habits.
Engineered resistance and risk assessment associated with insecticidal and weeds resistant transgenic cotton using wister rat model
Authors: Iqbal Adnan , Ali Muhammad Azam , Ahmed Shafique , Hassan Samina , Shahid Naila , Azam Saira , Rao Abdul Qayyum , Ali Qurban , Shahid Ahmad Ali 
Published: February 2022

AbstractStacking multiple genes into cotton crop to cop up multiple biotic stresses such as insects and weeds is a promising tool to save crop from losses. Transgenic cotton variety, VH-289, with double Bt and cp4EPSPS genes under the control of 35S promoter was used for the expression analyses and biosafety studies. The transgenic cotton plants were screened through PCR amplification of fragments, 1.7 kb for Cry1Ac, 582 bp for Cry2A and 250 bp for cp4EPSPS; which confirmed the presence of all genes transformed in transgenic cotton. The Cry1Ac + Cry2A and cp4EPSPS proteins were quantified through ELISA in transgenic cotton plants. The Glyphosate assay performed by spraying 1900 mL per acre of glyphosate Roundup further confirmed complete survival of transgenic cotton plants as compared to the non-transgenic cotton plants and all weeds. Similarly, insect infestation data determined that almost 99% insect mortality was observed in controlled field grown transgenic cotton plants as compared to the non-transgenic control plants. Evaluation of effect of temperature and soil nutrients availability on transgene expression in cotton plants was done at two different cotton growing regions, Multan and Lahore, Pakistan and results suggested that despite of higher temperature in Multan field, an increased level of Cry and cp4EPSPS proteins was recorded due to higher soil organic matter availability compared to Lahore field. Before commercialization of any transgenic variety its biosafety study is mandatory so, a 90 days biosafety study of the transgenic cotton plants with 40% transgenic cottonseeds in standard diet showed no harmful effect on wister rat model when studied for liver function, renal function and serum electrolyte.
Association between height loss and cardiovascular disease in the Korean elderly
Authors: Choi Soo Jung , Lee Rugyeom , Na Yewon , Hwang In Cheol , Jung Jaehun 
Published: February 2022

AbstractShorter people are at risk for cardiovascular disease (CVD), but data remain limited. This study sought to determine whether height loss is associated with an increased incidence of CVD. From the Korean National Health Insurance Service—Senior database (2002–2015), data of 134,952 individuals with available information on height loss was obtained. Height loss as percentages was measured 3–5 years from the baseline height. To assess hazard ratios for CVD incidence, multivariable Cox proportional hazard regression models were used before and after applying propensity score matching. The unmatched cohort consisted of 109,546 participants without height loss (< 1%): 20,208 participants with 1–2% height loss, and 5126 participants with ≥ 2% height loss. During a median follow-up period of 6.5 years (interquartile range, 3.7–8.5 years), 21,921 were newly diagnosed with CVD. Adults with height loss of > 2% had a greater risk of incident CVD than those with no height loss. This finding was statistically significant both in the original- and propensity score-matched cohorts. The increased risk for ischemic stroke was significant in the male subgroups, in line with degree of height loss. Overall, height loss is associated with an increased risk of subsequent ischemic stroke in Korean men.
Exploration of autoantibody responses in canine diabetes using protein arrays
Authors: O’Kell Allison L. , Shome Mahasish , Qiu Ji , Williams Stacy , Chung Yunro , LaBaer Joshua , Atkinson Mark A. , Wasserfall Clive 
Published: February 2022

AbstractCanine diabetes has been considered a potential model of human type 1 diabetes (T1D), however the detection of autoantibodies common in humans with T1D in affected dogs is inconsistent. The aim of this study was to compare autoantibody responses in diabetic and healthy control dogs using a novel nucleic acid programmable protein array (NAPPA) platform. We performed a cross-sectional study of autoantibody profiles of 30 diabetic and 30 healthy control dogs of various breeds. Seventeen hundred human proteins related to the pancreas or diabetes were displayed on NAPPA arrays and interrogated with canine sera. The median normalized intensity (MNI) for each protein was calculated, and results were compared between groups to identify candidate autoantibodies. At a specificity of 90%, six autoantibodies had sensitivity greater than 10% (range 13–20%) for distinguishing diabetic and control groups. A combination of three antibodies (anti-KANK2, anti-GLI1, anti-SUMO2) resulted in a sensitivity of 37% (95% confidence interval (CI) 0.17–0.67%) at 90% specificity and an area under the receiver operating characteristics curve of 0.66 (95% CI 0.52–0.80). While this study does not provide conclusive support for autoimmunity as an underlying cause of diabetes in dogs, future studies should consider the use of canine specific proteins in larger numbers of dogs of breeds at high risk for diabetes.
Titanium dioxide particles frequently present in face masks intended for general use require regulatory control
Authors: Verleysen Eveline , Ledecq Marina , Siciliani Lisa , Cheyns Karlien , Vleminckx Christiane , Blaude Marie-Noelle , De Vos Sandra , Brassinne Frédéric , Van Steen Frederic , Nkenda Régis , Machiels Ronny , Waegeneers Nadia , Van Loco Joris , Mast Jan 
Published: February 2022

AbstractAlthough titanium dioxide (TiO2) is a suspected human carcinogen when inhaled, fiber-grade TiO2 (nano)particles were demonstrated in synthetic textile fibers of face masks intended for the general public. STEM-EDX analysis on sections of a variety of single use and reusable face masks visualized agglomerated near-spherical TiO2 particles in non-woven fabrics, polyester, polyamide and bi-component fibers. Median sizes of constituent particles ranged from 89 to 184 nm, implying an important fraction of nano-sized particles (< 100 nm). The total TiO2 mass determined by ICP-OES ranged from 791 to 152,345 µg per mask. The estimated TiO2 mass at the fiber surface ranged from 17 to 4394 µg, and systematically exceeded the acceptable exposure level to TiO2 by inhalation (3.6 µg), determined based on a scenario where face masks are worn intensively. No assumptions were made about the likelihood of the release of TiO2 particles itself, since direct measurement of release and inhalation uptake when face masks are worn could not be assessed. The importance of wearing face masks against COVID-19 is unquestionable. Even so, these results urge for in depth research of (nano)technology applications in textiles to avoid possible future consequences caused by a poorly regulated use and to implement regulatory standards phasing out or limiting the amount of TiO2 particles, following the safe-by-design principle.
Ultrahigh SERS activity of the TiO2@Ag nanostructure leveraged for accurately detecting CTCs in peripheral blood
Authors: Xu Yanping , Zhang Dinghu , Lin Jie , Wu Xiaoxia , Xu Xiawei , Akakuru Ozioma Udochukwu , Zhang Hao , Zhang Zhewei , Xie Yujiao , Wu Aiguo , Shao Guoliang 
Published: February 2022

Accurately recognizing CTCs in peripheral blood based on the TiO2@Ag–R6G–rBSA–FA SERS bioprobe is carefully verified by in situ immunofluorescence staining experiments, which directly supports the CTC detection accuracy of the SERS strategy.
Control and capacity planning for energy storage systems to enhance the stability of renewable generation under weak grids
Authors: Guo Zixuan , Zhang Xing , Li Ming , Wang Hanyu , Han Feng , Fu Xinxin , Wang Jilei 
Published: February 2022

AbstractCurrent‐controlled inverters (CCIs), often used in renewable power generation, are prone to harmonic instability under weak grids with a low short‐circuit ratio (SCR). This paper proposes that this type of instability can be prevented by configuring part of the energy storage system (ESS) converters in the distribution network as voltage‐controlled inverters (VCIs). The main contributions of this paper are as follows: (1) A model of the CCI/VCI hybrid grid‐connected system is established, which can characterize not only the plant‐level stability, but also the unit‐level stability. (2) A rapid power control method for VCIs is proposed, which can improve the power control bandwidth of VCIs to tens of Hz to meet the rapidity requirements for ESS power control. (3) A capacity planning method is proposed, which can give the required minimum VCI/ESS capacity for a specific stability margin and SCR range. Analysis shows that as long as the ESS converters with a capacity of no less than 6% of the CCIs are configured as VCIs, large‐scale CCIs that are originally critically stable at SCR = 1.9 can operate stably under the very weak grid with SCR = 1.2. Finally, the proposed method is verified by simulations and experiments.
Micro- and millimeter-wave spectra of five conformers of cysteamine and their interstellar search
Authors: Song Wentao , Maris Assimo , Rivilla Víctor M. , Fortuna Francesca , Evangelisti Luca , Lv Dingding , Rodríguez-Almeida Lucas , Jiménez-Serra Izaskun , Martín-Pintado Jesús , Melandri Sonia 
Published: February 2022

Context. Cysteamine (NH2CH2CH2SH), a molecule of potential astrobiological interest, has not yet been detected in the interstellar medium. Furthermore, the sulfur- stituted isomer of ethanolamine (or 2-aminoethanol) has been recently detected in the molecular cloud G+0.693–0.027.
Aims. In order to conduct a new interstellar search for cysteamine in the molecular cloud G+0.693–0.027, its pure rotational spectrum needs to be investigated in the laboratory.
Methods. A pulsed-jet Fourier transform microwave spectrometer and a Stark-modulated free-jet millimeter-wave absorption spectrometer were used to measure the purely rotational spectrum of cysteamine in the range of 6.5-18 GHz (46.12–16.66 mm) and 59.6–120.0 GHz (5.03–2.72 mm), respectively. We used a deep spectral line survey toward the molecular cloud G+0.693-0.027 obtained with the IRAM 30 m and Yebes 40 m radiotelescopes to search for cysteamine.
Results. We assigned 815 rotational transition lines of five conformers (gGt, gGg, g′Gg, g′Gg′, and g'Gt) to fit the rotational constants, quartic centrifugal distortion constants, and the 14N nuclear quadrupole coupling constants. For four conformers (gGt, gGg, g′Gg, and g′Gg′), the 34S isotopologs were observed, and for two of them (gGg and g'Gg), the 13C and 15N isotopolog spectra were also detected; all in natural abundance. The five conformers of cysteamine were not detected toward the G+0.693–0.027 molecular cloud. We derived upper limits for their molecular abundances compared to molecular hydrogen of <(0.2–1.3) × 10–10. The relative abundances with respect to the oxygen counterpart ethanolamine, previously detected toward this cloud, are NH2CH2CH2OH/NH2CH2CH2SH > 0.8–5.3.
Fibro-ossärer Pseudotumor der ersten Kommissur – ein interessanter Fall
Authors: Fellmann Adrian , Calcagni Maurizio , Besmens Inga Swantje 
Published: February 2022

FallberichtEin 52-jähriger Patient stellte sich nach Zuweisung durch seinen Hausarzt aufgrund einer schmerzhaften, größenprogredienten Schwellung in der ersten Kommissur seiner linken adominanten Hand in der handchirurgischen Sprechstunde vor. Er gab an, die Schwellung vor fünf Wochen erstmals bemerkt zu haben und dass sie zunehmend schmerzhaft und funktionell einschränkend sei. Ein aktuell auslösendes Trauma wurde verneint; allerdings habe er sich vor drei Jahren bei einem Fahrradsturz im Bereich der betroffenen 1. Kommissur verletzt. Die Familienanamnese war bzgl. maligner Erkrankungen blande.
Erhöhtes Alter, kardiovaskuläre Nebenerkrankungen, COPD und Diabetes mellitus bedingen eine Übersterblichkeit in der septischen Unfallchirurgie
Authors: Maurer Elke , Wallmeier Vera , Reumann Marie , Ehnert Sabrina , Ihle Christoph , Schreiner Anna J , Flesch Ingo , Stollhof Laura Emine , Histing Tina , Nüssler Andreas K 
Published: February 2022

Zusammenfassung
Hintergrund Die konstante Überalterung der Bevölkerung in Deutschland führt u.a. zu einer Zunahme des durchschnittlichen Alters hospitalisierter Patienten. Hiermit einher gehen
eine reduzierte physiologische Reserve und ein reduzierter körpereigener Abwehrmechanismus und folglich eine gesteigerte Infekt- und Komplikationsanfälligkeit. Die Altersentwicklung der
septischen Unfallchirurgie ist im Vergleich zur Unfallchirurgie wenig erforscht. Zudem ist der Einfluss des Alters, verschiedener Vorerkrankungen, aber auch der des Alkohol- und
Nikotinkonsums auf die Mortalität in der septischen Unfallchirurgie unzureichend untersucht.
Methode 2014/15 (Exam1) wurden 345 Patienten der septischen Unfallchirurgie in die Studie eingeschlossen. 2017/18 (Exam2) erfolgte das 3-Jahres-Follow-up. Die Befragungen (Exam1
und 2) umfassten demografische Parameter, Nebenerkrankungen, die Medikamenteneinnahme, Alkohol- und Nikotinkonsum sowie verschiedene Parameter zur Morbidität. Hieraus wurde das
Sterberisiko in der septischen Unfallchirurgie in Abhängigkeit von den verschiedenen Risikofaktoren (Alter, Nebenerkrankungen, Medikamenteneinnahme und Noxen) berechnet. Zudem wurde die
Entwicklung des Patientenalters in der Unfallchirurgie sowie speziell in der septischen Unfallchirurgie zwischen 2010 und 2019 ausgewertet.
Ergebnisse 2014/15 (Exam1) wurden 345 Patienten in die Studie eingeschlossen. Hiervon konnten 2017/18 274 (79,4%) telefonisch erreicht werden. 36 (10,4%) lehnten eine Nachbefragung
ab. 20 (8,4%) der 238 verbleibenden Probanden waren bereits verstorben, 218 (63,2%) nahmen erneut teil. Zwischen 2010 (n = 492) und 2019 (n = 885) nahm die Patientenzahl der septischen
Unfallchirurgie in unserer Abteilung um 79,9% zu, wobei besonders der Anteil der unter 65-Jährigen anstieg. Ab dem 60. Lebensjahr stieg die Sterbewahrscheinlichkeit (60. Lj: 0,0377 vs. 70.
Lj: 0,1395) sprunghaft an; wobei in der septischen Unfallchirurgie die eines 60-Jährigen der Sterbewahrscheinlichkeit eines 80-Jährigen der Normalbevölkerung entsprach. Nikotin- (p = 0,93)
und Alkoholkonsum (p = 0,344) zeigten keinen signifikanten Einfluss auf die Sterblichkeit, während kardiale Vorerkrankungen (p = 0,01), die COPD (p = 0,01), der Diabetes mellitus
(p = 0,05) und die pAVK (p = 0,01) mit einer signifikant erhöhten Sterblichkeit einhergingen.
Schlussfolgerung Das Alter, kardiale Vorerkrankungen, aber auch die COPD, der Diabetes mellitus und die periphere arterielle Verschlusskrankheit gehen mit einer signifikant
erhöhten Sterblichkeit in der septischen Unfallchirurgie einher. Eine exponentielle Zunahme des Sterblichkeitsrisikos besteht zwischen dem 50. und 60. Lebensjahr, wobei das Risiko zu
versterben eines 60-jährigen Patienten der septischen Unfallchirurgie dem eines 80-jährigen aus der Normalbevölkerung entspricht.
Usefulness of endoscopic ultrasound in children with pancreatobiliary and gastrointestinal symptoms
Authors: Dalal Ankit , Kamat Nagesh , Patil Gaurav , Daftary Rajen , Maydeo Amit 
Published: February 2022

Abstract
Background and study aims Endoscopic ultrasound (EUS) is useful in diagnosing and treating childhood pancreatobiliary and gastrointestinal diseases. However, there are limited data on its effectiveness for various indications.
Patients and methods This was a retrospective analysis of prospectively collected data of patients who underwent EUS for upper gastrointestinal tract disorders from January 2018 to December 2020 to assess its indications, findings, interventions, and complications.
Results Ninety-two procedures were performed in 85 children, (70.5 % male; mean [SD] age 12.1 years [3.9] years) with a mean (SD) symptom duration of 1.1 (0.5) years. The procedures were technically successful in all patients. The primary indication for EUS was abdominal pain in 45(52.9%) and jaundice/cholangitis in 15 patients (17.6 %). General anesthesia was used in 12 (13 %) and TIVA in 80 patients (87 %). The most common diagnostic findings were choledocholithiasis in 21 (24.7 %) and cholelithiasis in 12 patients (14.1 %). Among interventions, EUS-guided cystogastrostomy for pancreatic pseudocyst was done in four patients (4.7 %), and EUS-guided rendezvous for failed ERCP in one patient (1.2 %) with cholangitis. There were no immediate post-procedural complications. Overall, EUS had a meaningful impact on the subsequent clinical management in 69 cases (81.2 %).
Conclusions EUS in the pediatric population is safe, effective, and has a meaningful impact in appropriately selected cases. It can act as a rescue in major therapeutic procedures, but adequate care should be taken at the procedural level and during anesthesia.