bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment and Molecular Modeling Study of Cyclodextrin-Based Synergistic Enantioseparation Systems with Three New Amino Acid Chiral Ionic Liquids as Additives in Capillary Electrophoresis
Authors: Liu Jie , Zhang Jian , Zhu Dongyang , Zhu Xinqi , Du Yingxiang , Ma Xiaofei , Feng Zijie , Sun Xiaodong , Xu Hui 
Published: June 2022

Abstract
Chiral ionic liquids (CILs) have attracted more and more attention due to their superior performance as chiral additives in capillary electrophoresis. In this work, based on the cyclodextrin (CD) derivatives and three new amino acid CILs (trifluoroacetate-L-Hydroxyproline, nitric acid-L-Hydroxyproline and trifluoroacetate-L-threonine), the new synergistic systems were established for chiral drug separation. In contrast to the traditional single glucosyl-β-CD (Glu-β-CD) separation system, the CIL/Glu-β-CD synergistic systems achieved improved resolution of three model drug racemates. Some experimental variables, such as CIL concentration, Glu-β-CD concentration, buffer pH, applied voltage, and the type and proportion of organic modifier, were optimized in the trifluoroacetate-L-Hydroxyproline/Glu-β-CD synergistic system. In addition, the recognition process in the synergistic system was studied through the molecular modeling method.
Prognostic impact of carcinoembryonic antigen in 1822 surgically treated esophageal squamous cell carcinoma: multi-institutional study of the Japan Esophageal Society
Authors: Suzuki Takashi , Yajima Satoshi , Okamura Akihiko , Yoshida Naoya , Taniyama Yusuke , Murakami Kentaro , Ohkura Yu , Nakajima Yasuaki , Yagi Koichi , Fukuda Takashi , Ogawa Ryo , Hoshino Isamu , Kunisaki Chikara , Narumiya Kosuke , Tsubosa Yasuhiro , Yamada Kazuhiko , Shimada Hideaki ,   Japan Esophageal Society Promotion Research: p53 Antibody Multicenter Research Group
Published: June 2022

Abstract

Background
Previous studies have evaluated the clinicopathological significance of carcinoembryonic antigen (CEA) of esophageal cancer in relatively small numbers of patients. Therefore, this study aimed to clarify the prognostic significance of CEA in 1822 patients with esophageal squamous cell carcinoma (SCC).

Methods
Based on the Japanese Esophageal Society nationwide multi-institutional retrospective study, a total of 1,748 surgically treated ESCC from 15 hospitals were enrolled to evaluate prognostic impact of preoperative CEA values. Among them, 605 patients were categorized to up-front surgery group, and 1,217 patients were categorized to neoadjuvant therapy group. The CEA threshold for positivity was 3.7 ng/ml. The clinicopathological and prognostic impact of CEA was evaluated by univariate and multivariate analysis in each treatment modality groups.

Results
In total, the CEA positive rate was 25.8% (470/1822). CEA-positive status was significantly associated with distant metastasis (P = 0.004) but not associated with other factors. CEA-positive status was associated with poor overall survival (P &lt; 0.001) in univariate analysis as well as multivariate analysis (P = 0.003).

Conclusions
CEA was an independent prognostic determinant of overall survival in esophageal SCC. Based on the subgroup analysis, regardless of the treatment modality, patients with high pretreatment CEA showed poor overall survival.
Extracellular ST6GAL1 regulates monocyte–macrophage development and survival
Authors: Rusiniak Michael E , Punch Patrick R , Hait Nitai C , Maiti Aparna , Burns Robert T , Chapla Digantkumar , Moremen Kelley W , Zhao Peng , Wells Lance , Hoffmeister Karin , Lau Joseph T Y 
Published: June 2022

Abstract
Interaction of immune cells with the systemic environment is necessary for the coordinated development and execution of immune responses. Monocyte-macrophage lineage cells reside at the junction of innate and adaptive immunity. Previously we reported that the sialyltransferase ST6GAL1 in the extracellular milieu modulates B cell development and IgG production, granulocyte production, and attenuates acute airway inflammation to bacterial challenge in mouse models. Here, we report that extracellular ST6GAL1 also elicits profound responses in monocyte-macrophage lineage cells. We show that recombinant ST6GAL1 adheres to subsets of thioglycolate-elicited inflammatory cells in the mouse peritoneum and to cultured human monocyte THP-1 cells. Exposure of the inflammatory cells to recombinant ST6GAL1 elicited wholesale changes in the gene expression profile of primary mouse myeloid cells; most notable was the striking up-regulation of monocyte-macrophage and monocyte-derived dendritic cell development pathway signature genes and transcription factors PU.1, NFκB and their target genes, driving increased monocyte-macrophage population and survival ex vivo. In the cultured human monocyte cells, the essential cell surface receptor of the monocyte-macrophage lineage, the M-CSF receptor (M-CSF-R, Csfr1) was a target of extracellular ST6GAL1 catalytic activity. Extracellular ST6GAL1 activated the M-CSF-R and initiated intracellular signaling events, namely, the nuclear translocation of NFκB subunit p65, and phosphorylation of ERK 1/2 and AKT. The findings implicate extracellular ST6GAL1 in monocyte development by a mechanism initiated at the cell surface and support an emerging paradigm of an extracellular glycan-modifying enzyme as a central regulator coordinating immune hematopoietic cell development and function.
The critical role of the TB5 domain of fibrillin-1 in endochondral ossification
Authors: Delhon Laure , Mougin Zakaria , Jonquet Jérémie , Bibimbou Angélique , Dubail Johanne , Bou-Chaaya Cynthia , Goudin Nicolas , Le Goff Wilfried , Boileau Catherine , Cormier-Daire Valérie , Le Goff Carine 
Published: June 2022

Abstract
Mutations in the fibrillin-1 (FBN1) gene are responsible for the autosomal dominant form of geleophysic dysplasia (GD), which is characterized by short stature and extremities, thick skin and cardiovascular disease. All known FBN1 mutations in patients with GD are localized within the region encoding the transforming growth factor-β binding protein-like 5 (TB5) domain of this protein. Herein, we generated a knock-in mouse model, Fbn1Y1698C by introducing the p.Tyr1696Cys mutation from a patient with GD into the TB5 domain of murine Fbn1 to elucidate the specific role of this domain in endochondral ossification. We found that both Fbn1Y1698C/+ and Fbn1Y1698C/Y1698C mice exhibited a reduced stature reminiscent of the human GD phenotype. The Fbn1 point mutation introduced in these mice affected the growth plate formation owing to abnormal chondrocyte differentiation such that mutant chondrocytes failed to establish a dense microfibrillar network composed of FBN1. This original Fbn1 mutant mouse model offers new insight into the pathogenic events underlying GD. Our findings suggest that the etiology of GD involves the dysregulation of the extracellular matrix composed of an abnormal FBN1 microfibril network impacting the differentiation of the chondrocytes.
Revisiting nuclear hedging: ballistic missiles and the Iranian example
Authors: Hiim Henrik Stålhane 
Published: June 2022

Abstract
Technological shifts have made nascent nuclear arsenals more vulnerable. This article examines how this affects nuclear hedgers—that is, states deliberately seeking nuclear latency or the capability to develop the bomb. It argues that growing vulnerabilities provide hedgers with increasing incentives to acquire sophisticated ballistic missiles. Possession of missiles is crucial to limit the ‘window of vulnerability’ between a nuclear breakout decision and the attainment of a nuclear arsenal with deterrence value. The article illustrates this tendency through a case-study of the Iranian missile and rocket programs. It finds that missile acquisition has indeed been an integral part of Iran's hedging strategy, and that several of the systems it has acquired indicate an interest in nuclear weapons delivery. Furthermore, Iran's sophisticated missile arsenal significantly shortens its path to a credible nuclear deterrent. The article contributes to the proliferation literature, which has overlooked that the development of missiles is an increasingly important element of contemporary nuclear hedging. Its findings show that missiles increasingly need to be considered in scholarly assessments of states' nuclear latency and hedging, and in discussions about how difficult it is to acquire nuclear weapons. Moreover, the findings indicate that practitioners working to prevent proliferation should prioritize tracking missile programs.
Impact of the Early COVID-19 Pandemic on Burn Care: A Multi-National Study
Authors: Vasudevan Ananya , Bailey Hannah M , Sager Alan , Kazis Lewis E 
Published: June 2022

Abstract
This qualitative study documents and analyzes COVID-19’s impacts on burn care in twelve nations. Burn care saw valuable improvements in surgical techniques, skin grafting, and other acute treatments during the decades before the COVID-19 pandemic which increased severely burned patients’ survival rates and quality of life. Detailed interviews, conducted in the 12 nations reveal that the COVID-19 pandemic greatly affected the delivery of acute and rehabilitation services for burn patients. Resources have been suctioned away from non-COVID healthcare and burn care has not been spared. Acute, post-acute rehabilitation, and mental health services have all suffered. Weak preparation has deeply burdened health care services in most nations, resulting in lower access to care. Access problems have accelerated innovations like telehealth in many nations. The spread of misinformation through social and traditional media has contributed to the varied responses to COVID-19. This compounded problems in health care delivery. Burn care providers delivered services for survivors during extremely difficult circumstances by continuing to furnish acute and long-term services for patients with complex disease. Emphasis on future pandemic preparedness will be vital because they undermine all aspects of burn care and patient outcomes. As the COVID-19 pandemic continues to unfold around the world, it will be important to continue to monitor and analyze the adaptability of nations, health care systems, and burn care providers.
Impact of adherence to criteria on oncological outcomes of radical prostatectomy in patients opting for active surveillance: data from the PRIAS-JAPAN study
Authors: Tohi Yoichiro , Kato Takuma , Miyakawa Jimpei , Matsumoto Ryuji , Sasaki Hiroshi , Mitsuzuka Koji , Inokuchi Junichi , Matsumura Masafumi , Yokomizo Akira , Kinoshita Hidefumi , Hara Isao , Kawamura Norihiko , Hashimoto Kohei , Inoue Masaharu , Teishima Jun , Kanno Hidenori , Fukuhara Hiroshi , Maruyama Satoru , Sakamoto Shinichi , Saito Toshihiro , Kakehi Yoshiyuki , Sugimoto Mikio 
Published: June 2022

Abstract

Objectives
This study aimed to evaluate whether oncological outcomes of radical prostatectomy differ depending on adherence to the criteria in patients who opt for active surveillance.

Materials and methods
We retrospectively reviewed the data of 1035 patients enrolled in a prospective cohort of the PRIAS-JAPAN study. After applying the exclusion criteria, 136 of 162 patients were analyzed. Triggers for radical prostatectomy due to pathological reclassification on repeat biopsy were defined as on-criteria. Off-criteria triggers were defined as those other than on-criteria triggers. Unfavorable pathology on radical prostatectomy was defined as pathological ≥T3, ≥GS 4 + 3 and pathological N positivity. We compared the pathological findings on radical prostatectomy and prostate-specific antigen recurrence-free survival between the two groups. The off-criteria group included 35 patients (25.7%), half of whom received radical prostatectomy within 35 months.

Results
There were significant differences in median prostate-specific antigen before radical prostatectomy between the on-criteria and off-criteria groups (6.1 vs. 8.3 ng/ml, P = 0.007). The percentage of unfavorable pathologies on radical prostatectomy was lower in the off-criteria group than that in the on-criteria group (40.6 vs. 31.4%); however, the differences were not statistically significant (P = 0.421). No significant difference in prostate-specific antigen recurrence-free survival was observed between the groups during the postoperative follow-up period (median: 36 months) (log-rank P = 0.828).

Conclusions
Half of the off-criteria patients underwent radical prostatectomy within 3 years of beginning active surveillance, and their pathological findings were not worse than those of the on-criteria patients.
Performing the Self through Orientalizing the Kurds in Isabella Bird’s Journeys in Persia and Kurdistan
Authors: Ghaderi Farah , Heidari Himan 
Published: June 2022

Abstract
This article fleshes out the various ways Isabella Bird performs the self in her travel account, Journeys in Persia and Kurdistan (1891), mainly in her engagement with the Kurdish people. Deploying Judith Butler’s theory of performativity of gender, we argue that travel writing is empowering for Bird because it offers her a viable platform to perform a variety of selves through which she can voice her complicated and nuanced socio-political views and promote her image. Moreover, we contend that Bird’s representation of the Kurds and their region is informed by Orientalist ideology of the time as well as her own complex subject position. The fluidity of Bird’s identity, which is represented through performing a rich diversity of masculine and feminine selves in her account, exposes the constructed nature of gender. Bird not only undermines the prescribed gender boundaries of her time, but also demands the right for herself, as a woman writer, to be both caring and daring by playing the roles of a brave traveller, intellectual explorer, devoted Hakim, shrewd political analyst, religious commentator, and receptive ethnographer in Journeys.
New insights into the role of melatonin in photosynthesis
Authors: Yang Sijia , Zhao Yuqing , Qin Xiaolong , Ding Chunbang , Chen Yanger , Tang Zizhong , Huang Yan , Reiter Russel J , Yuan Shu , Yuan Ming 
Published: June 2022

Abstract
There are numerous studies on enhancing plant resistance to stress using melatonin, but few studies about its effect on photosynthesis. Herein, we summarized the role of melatonin in photosynthesis. Melatonin regulates chlorophyll synthesis and degradation through the transcription of related genes and hormone signals. It protects photosynthetic proteins and maintains the photosynthetic process through improving the transcription of photosystem genes, activating the antioxidant system, and promoting the xanthophyll cycle. Melatonin potentially regulates plant stomatal movement through CAND2/PMTR1. Finally, it controls the photosynthetic carbon cycle by regulating the metabolism of sugar, the gluconeogenesis pathway, and the degradation and transport of transient starch.
miR156 regulates somatic embryogenesis by modulating starch accumulation in citrus
Authors: Feng Meng-Qi , Lu Meng-Di , Long Jian-Mei , Yin Zhao-Ping , Jiang Nan , Wang Peng-Bo , Liu Yue , Guo Wen-Wu , Wu Xiao-Meng 
Published: June 2022

Abstract
Somatic embryogenesis (SE) is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156-SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156-SPL module in SE remains unclear. In this study, we found that transcription factors CsAGL15 and CsFUS3 bind to the CsMIR156A promoter and activate its expression. Suppression of csi-miR156a function leads to up-regulation of four target genes, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (CsSPL) genes, and reduction of SE efficiency. In the short tandem target mimic (STTM)-miR156a overexpression callus (MIM156), the number of amyloplasts and starch content were significantly reduced, and genes involved in starch synthesis and transport were down-regulated. csi-miR172d was down-regulated, whereas the target genes, CsTOE1.1 and CsTOE1.2, which inhibit the expression of starch biosynthesis genes, were up-regulated. In our working model, CsAGL15 and CsFUS3 activate csi-miR156a, which represses CsSPLs and further regulates csi-miR172d and CsTOEs, thus altering starch accumulation in callus cells and regulating SE in citrus. This study elucidates the pathway of miR156-SPLs and miR172-TOEs-mediated regulation of SE, and provides new insights into enhancing SE capability in citrus.
Two homeologous MATE transporter genes,NtMATE21andNtMATE22, are involved in the modulation of plant growth and flavonol transport inNicotiana tabacum
Authors: Gani Umar , Nautiyal Abhishek Kumar , Kundan Maridul , Rout Biswaranjan , Pandey Ashutosh , Misra Prashant 
Published: June 2022

AbstractThe multidrug and toxic compound extrusion (MATE) protein family has been implicated in the transport of a diverse range of molecules, including specialized metabolites. In tobacco (Nicotiana tabacum), only a limited number of MATE transporters have been functionally characterized, and no MATE transporter has been studied in the context of flavonoid transport in this plant species so far. In the present study, we characterize two homeologous tobacco MATE genes, NtMATE21 and NtMATE22, and demonstrate their role in flavonol transport and in plant growth and development. The expression of these two genes was reported to be up-regulated in trichomes as compared with the trichome-free leaf. The transcript levels of NtMATE21 and NtMATE22 were found to be higher in flavonol overproducing tobacco transgenic lines as compared with wild type tobacco. The two transporters were demonstrated to be localized to the plasma membrane. Genetic manipulation of NtMATE21 and NtMATE22 led to altered growth phenotypes and modulated flavonol contents in N. tabacum. The β-glucuronidase and green fluorescent protein fusion transgenic lines of promoter regions suggested that NtMATE21 and NtMATE22 are exclusively expressed in the trichome heads in the leaf tissue and petals. Moreover, in a transient transactivation assay, NtMYB12, a flavonol-specific MYB transcription factor, was found to transactivate the expression of NtMATE21 and NtMATE22 genes. Together, our results strongly suggest the involvement of NtMATE21 and NtMATE22 in flavonol transport as well as in the regulation of plant growth and development.
On the energy dependence of the QPO phenomenon in the black hole system MAXI J1535-571
Authors: Garg Akash , Misra Ranjeev , Sen Somasri 
Published: June 2022

ABSTRACT
Previous analysis of AstroSat observations of the black hole system MAXI J1535-571 have revealed the presence of a strong Quasi-Periodic Oscillation (QPO) whose frequency is correlated with the high energy spectral index. Here, we fit the spectra as emitted from a truncated disc with an inner hot corona, study the QPO frequency dependence on other spectral parameters and model the energy-dependent r.m.s and time-lag of the QPO to identify the physical spectral parameters whose variation are responsible for the QPO. The QPO frequency is found to also correlate with the scattering fraction (i.e. the fraction of the soft photons Comptonized) and its dependence on the accretion rate and inner disc radii is consistent with it being the dynamical frequency. The time-lag between the hard and soft photons is negative for QPO frequency &gt;2.2 Hz and is positive for lesser values, making this the second black hole system to show this behaviour after GRS 1915+105. Modelling the energy-dependent time-lag and r.m.s requires correlated variation of the accretion rate, inner disc radii, and the coronal heating rate, with the latter having a time-lag compared to the other two for QPO frequencies less than &lt;2.2 Hz and which changes sign (i.e. the coronal heating variation precedes the accretion rate one) for higher values. The implications of the results are discussed.
Constraining baryonic feedback and cosmology with weak-lensing, X-ray, and kinematic Sunyaev–Zeldovich observations
Authors: Schneider Aurel , Giri Sambit K , Amodeo Stefania , Refregier Alexandre 
Published: June 2022

ABSTRACT
Modern weak-lensing observations are becoming increasingly sensitive to baryonic feedback processes which are still poorly understood. So far, this challenge has been faced either by imposing scale-cuts in the data or by modelling baryonic effects with simple, one-parameter models. In this paper, we rely on a more general, seven-parameter prescription of baryonic feedback effects, which is primarily motivated by observations and has been shown to agree with a plethora of hydrodynamical simulations. By combining weak-lensing data from the Kilo-Degree Survey (KiDS-1000) with observations of gas around galaxy clusters, we are able to constrain baryonic parameters and learn more about feedback and cosmology. In particular, we use cluster gas fractions from X-ray data and gas profiles from kinematic Sunyaev–Zeldovich (kSZ) observations to provide evidence for baryonic feedback that is stronger than predicted by most hydrodynamical simulations. In terms of the matter power spectrum, we report a beyond-per cent effect at wave-modes above k ∼ 0.1–0.45 $h\, {\rm Mpc}^{-1}$ and a maximum suppression of 12–33 per cent at k ∼ 7 $h\, {\rm Mpc}^{-1}$ (68 per cent confidence level). Regarding the combined parameter Σ8 = σ8(Ωm/0.3)0.58, we find the known tension with the Planck satellite data to be reduced from 3.8σ to 2.9σ once baryonic effects are fully included in the analysis pipeline. The tension is further decreased to 2.6σ when the weak-lensing data are combined with X-ray and kSZ observations. We conclude that, while baryonic feedback effects become more important in modern weak-lensing surveys, they are unlikely to act as the main culprit for the observed Σ8-tension.
CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity
Authors: Marino Nicole D , Pinilla-Redondo Rafael , Bondy-Denomy Joseph 
Published: June 2022

Abstract
CRISPR-Cas12a (Cpf1) is a bacterial RNA-guided nuclease that cuts double-stranded DNA (dsDNA) at sites specified by a CRISPR RNA (crRNA) guide. Additional activities have been ascribed to this enzyme in vitro: site-specific (cis) single-stranded DNA (ssDNA) cleavage and indiscriminate (trans) degradation of ssDNA, RNA, and dsDNA after activation by a complementary target. The ability of Cas12a to cleave nucleic acids indiscriminately has been harnessed for many applications, including diagnostics, but it remains unknown if it contributes to bacterial immunity. Here, we provide evidence that cleavage of ssDNA in cis or in trans by Cas12a is insufficient to impact immunity. Using LbCas12a expressed in either Pseudomonas aeruginosa or Escherichia coli, we observed that cleavage of dsDNA targets did not elicit cell death or dormancy, suggesting insignificant levels of collateral damage against host RNA or DNA. Canonical immunity against invasive dsDNA also had no impact on the replicative fitness of co-infecting dsDNA phage, ssDNA phage or plasmid in trans. Lastly, crRNAs complementary to invasive ssDNA did not provide protection, suggesting that ssDNA cleavage does not occur in vivo or is insignificant. Overall, these results suggest that CRISPR-Cas12a immunity predominantly occurs via canonical targeting of dsDNA, and that the other activities do not significantly impact infection outcomes.