bluuebunny/crossref_metadata_2025_split huggingface dataset:
Demographic and psychosocial correlates of measurement error and reactivity bias in a 4-d image-based mobile food record among adults with overweight and obesity
Authors: Whitton Clare , Healy Janelle D. , Dhaliwal Satvinder S. , Shoneye Charlene , Harray Amelia J. , Mullan Barbara A. , McVeigh Joanne A. , Boushey Carol J. , Kerr Deborah A. 
Published: May 2022

AbstractImproving dietary reporting among people living with obesity is challenging as many factors influence reporting accuracy. Reactive Reporting may occur in response to dietary recording, but little is known about how image-based methods influence this process. Using a 4-d image-based mobile food record (mFRTM), this study aimed to identify demographic and psychosocial correlates of measurement error and reactivity bias, among adults with BMI 25–40 kg/m2. Participants (n 155, aged 18–65 years) completed psychosocial questionnaires and kept a 4-d mFRTM. Energy expenditure (EE) was estimated using ≥ 4 d of hip-worn accelerometer data, and energy intake (EI) was measured using mFRTM. EI:EE ratios were calculated, and participants in the highest tertile were considered to have Plausible Intakes. Negative changes in EI according to regression slopes indicated Reactive Reporting. Mean EI was 72 % (sd = 21) of estimated EE. Among participants with Plausible Intakes, mean EI was 96 % (sd = 13) of estimated EE. Higher BMI (OR 0·81, 95 % CI 0·72, 0·92) and greater need for social approval (OR 0·31, 95 % CI 0·10, 0·96) were associated with lower likelihood of Plausible Intakes. Estimated EI decreased by 3 % per d of recording (interquartile range − 14 %,6 %) among all participants. The EI of Reactive Reporters (n 52) decreased by 17 %/d (interquartile range − 23 %,–13 %). A history of weight loss (> 10 kg) (OR 3·4, 95 % CI 1·5, 7·8) and higher percentage of daily energy from protein (OR 1·1, 95 % CI 1·0, 1·2) were associated with greater odds of Reactive Reporting. Identification of reactivity to measurement, as well as Plausible Intakes, is recommended in community-dwelling studies to highlight and address sources of bias.
Italian Field Hospital Experience in Mozambique: Report of Ordinary Activities in an Extraordinary Context
Authors: Sacchetto Daniela , Raviolo Mario , Lovesio Silvia , Salio Flavio , Hubloue Ives , Ragazzoni Luca 
Published: May 2022

AbstractOn March 15, 2019, Cyclone Idai made landfall near the port city of Beira in central Mozambique causing significant casualties and serious damage to infrastructure. The Emergency Medical Team Type 2 – Italy Regione Piemonte (EMT2-ITA) was deployed approximately two weeks after the disaster to support the country in need, providing essential medical and surgical care.The EMT2-ITA staff was composed of 77 team members including two rotations and integrating local staff. A total of 1,121 patients (1,183 triage admissions) were treated during the 27 days of field hospital activity; among all the admissions, only few cases (17; 1%) were directly or indirectly attributed to the disaster event. Only three cases of cholera were confirmed and transferred to one of the treatment centers set up in Beira. The EMT2-ITA performed a total of 62 surgical operations (orthopedic, gynecological, general, and plastic surgery), of which more than one-half were elective procedures.The objective of this manuscript is to report the mission of the EMT2-ITA in Mozambique, raising interesting points of discussion regarding the impact of timing on the mission outcomes, the operational and clinical activities in the field hospital, and the great importance to integrate local staff into the team.
Confirmation of glufosinate-resistant Palmer amaranth and response to other herbicides
Authors: Priess Grant L , Norsworthy Jason K , Godara Navdeep , Mauromoustakos Andy , Butts Thomas R , Roberts Trenton L , Barber Tom 
Published: May 2022

AbstractThe ability of weed populations to evolve resistance to herbicides affects management strategies and the profitability of crop production. The objective of this research was to screen Palmer amaranth accessions from Arkansas for glufosinate resistance. Additional efforts focused on the effectiveness of various herbicides, across multiple sites of action (SOAs), on each putative-resistant accession. The three putative accessions were selected from 60 Palmer amaranth accessions collected in 2019 and 2020 and screened with to 0.5× and 1× rates of glufosinate. A dose-response experiment was conducted for glufosinate on accessions A2019, A2020, and B2020. The effectiveness of various preemergence- and postemergence-applied herbicides were evaluated on each accession. Resistance ratios of A2019, A2020, and B2020 to glufosinate ranged from 5.1 to 27.4 when comparing LD50 values to two susceptible accessions, thus all three accessions were resistant to glufosinate. All three accessions (A2019, A2020, and B2020) were found to have a reduction equal to or greater than 20 percentage points in mortality to at least one herbicide from five different SOAs equal to or greater than five sites of action. Herbicides from nine different SOAs controlled A2019 at least 20 percentage points less than the susceptible accessions, which points to a need for additional research to characterize the response of this accession.
Testing of the Seismic Gap Hypothesis in a Model With Realistic Earthquake Statistics
Authors: Petrillo G. , Rosso A. , Lippiello E. 
Published: May 2022

AbstractThe seismic gap hypothesis states that fault regions where no large earthquake has recently occurred, are more prone than others to host the next large earthquake. It can lead to the idea of immunity after local disaster which, notwithstanding it sounds reasonable, it has been frequently rejected by objective testing. More generally, the estimate of the occurrence probability of the next big shock on the basis of the time delay from the last earthquake still represents a big challenge. The problem is that this issue cannot be addressed only on the basis of historical catalogs which contain too few well documented big shocks, and decades of future observations appear necessary. On the other hand, recent results have shown that important insights can be obtained from the spatial organization of aftershocks and its relationship to the mainshock slip profile. Here, we address this issue by monitoring the stress evolution together with the occurrence of big shocks and their aftershocks in a model where the fault is described as an elastic interface embedded in a ductile medium. The model reproduces all relevant statistical features of earthquake occurrence and allows us to perform accurate testing of the seismic gap hypothesis and its consequences, particularly on the side of aftershock spatial patterns. We show that large earthquakes do not regularly repeat in time, but it is possible to achieve insights on the time until the next big shock from the percentage of aftershocks occurring inside the mainshock slip contour.
A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paper-based enzyme-linked immunosorbent assay
Authors: Lin Dong , Li Bowei , Fu Longwen , Qi Ji , Xia Chunlei , Zhang Yi , Chen Jiadong , Choo Jaebum , Chen Lingxin 
Published: May 2022

AbstractNitrocellulose (NC) membranes, as porous paper-like substrates with high protein-binding capabilities, are very popular in the field of point-of-care immunoassays. However, generating robust hydrophobic structures in NC membranes to fabricate microfluidic paper-based analytical devices (μPADs) remains a great challenge. At present, the main method relies on an expensive wax printer. In addition, NC membranes very easy to adhere during the printing process due to electrostatic adsorption. Herein, we developed a facile, fast and low-cost strategy to fabricate μPADs in NC membranes by screen-printing polyurethane acrylate (PUA) as a barrier material for defining flow channels and reaction zones. Moreover, hydrophobic barriers based on UV-curable PUA can resist various surfactant solutions and organic solvents that are generally used in immunoassays and biochemical reactions. To validate the feasibility of this PUA-based NC membrane for immunoassays in point-of-care testing (POCT), we further designed and assembled a rotational paper-based analytical device for implementing a multiplexed enzyme-linked immunosorbent assay (ELISA) in a simple manner. Using the proposed device under the optimal conditions, alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA) could be identified, with limits of detection of 136 pg/mL and 174 pg/mL, respectively, which are below the threshold values of these two cancer biomarkers for clinical diagnosis. We believe that this reliable device provides a promising platform for the diagnosis of disease based on ELISA or other related bioassays in limited settings or remote regions.
Heme cross-feeding can augment Staphylococcus aureus and Enterococcus faecalis dual species biofilms
Authors: Ch’ng Jun-Hong , Muthu Mugil , Chong Kelvin K L , Wong Jun Jie , Tan Casandra A Z , Koh Zachary J S , Lopez Daniel , Matysik Artur , Nair Zeus J , Barkham Timothy , Wang Yulan , Kline Kimberly A 
Published: May 2022

Abstract
The contribution of biofilms to virulence and as a barrier to treatment is well-established for Staphylococcus aureus and Enterococcus faecalis, both nosocomial pathogens frequently isolated from biofilm-associated infections. Despite frequent co-isolation, their interactions in biofilms have not been well-characterized. We report that in combination, these two species can give rise to augmented biofilms biomass that is dependent on the activation of E. faecalis aerobic respiration. In E. faecalis, respiration requires both exogenous heme to activate the cydAB-encoded heme-dependent cytochrome bd, and the availability of O2. We determined that the ABC transporter encoded by cydDC contributes to heme import. In dual species biofilms, S. aureus provides the heme to activate E. faecalis respiration. S. aureus mutants deficient in heme biosynthesis were unable to augment biofilms whereas heme alone is sufficient to augment E. faecalis mono-species biofilms. Our results demonstrate that S. aureus-derived heme, likely in the form of released hemoproteins, promotes E. faecalis biofilm formation, and that E. faecalis gelatinase activity facilitates heme extraction from hemoproteins. This interspecies interaction and metabolic cross-feeding may explain the frequent co-occurrence of these microbes in biofilm-associated infections.
The aspartyl protease DDI2 drives adaptation to proteasome inhibition in multiple myeloma
Authors: Op Mélanie , Ribeiro Sérgio T. , Chavarria Claire , De Gassart Aude , Zaffalon Léa , Martinon Fabio 
Published: May 2022

AbstractProteasome inhibitors, such as bortezomib, are first-line therapy against multiple myeloma (MM). Unfortunately, patients frequently become refractory to this treatment. The transcription factor NRF1 has been proposed to initiate an adaptation program that regulates proteasome levels. In the context of proteasome inhibition, the cytosolic protease DDI2 cleaves NRF1 to release an active fragment that translocates to the nucleus to promote the transcription of new proteasome subunits. However, the contribution of the DDI2-NRF1 pathway to bortezomib resistance is poorly understood. Here we show that upon prolonged bortezomib treatment, MM cells become resistant to proteasome inhibition by increasing the expression of DDI2 and consequently activation of NRF1. Furthermore, we found that many MM cells became more sensitive to proteasome impairment in the context of DDI2 deficiency. Mechanistically, we demonstrate that both the protease and the HDD domains of DDI2 are required to activate NRF1. Finally, we show that partial inhibition of the DDI2-protease domain with the antiviral drug nelfinavir increased bortezomib susceptibility in treated MM cells. Altogether, these findings define the DDI2-NRF1 pathway as an essential program contributing to proteasome inhibition responses and identifying DDI2 domains that could be targets of interest in bortezomib-treated MM patients.
NF-κB-inducing kinase (NIK) is activated in pancreatic β-cells but does not contribute to the development of diabetes
Authors: Xiao Peng , Takiishi Tatiana , Violato Natalia Moretti , Licata Giada , Dotta Francesco , Sebastiani Guido , Marselli Lorella , Singh Sumeet Pal , Sze Mozes , Van Loo Geert , Dejardin Emmanuel , Gurzov Esteban Nicolas , Cardozo Alessandra Kupper 
Published: May 2022

AbstractThe transcription factor nuclear factor-κB (NF-κB) has a key role in the pathogenesis of diabetes and its complications. Although activation of the canonical NF-κB pathway in β-cells is generally deleterious, little is known about the role of the non-canonical NF-κB signalling and its main regulator, the NF-κB-inducing kinase (NIK), on pancreatic β-cell survival and function. Previous studies based on models of NIK overexpression in pancreatic islet cells showed that NIK induced either spontaneous β-cell death due to islet inflammation or glucose intolerance during diet-induced obesity (DIO) in mice. Therefore, NIK has been proposed as a potential target for diabetes therapy. However, no clear studies showed whether inhibition of NIK improves diabetes development. Here we show that genetic silencing of NIK in pancreatic β-cells neither modifies diabetes incidence nor inflammatory responses in a mouse model of immune-mediated diabetes. Moreover, NIK silencing in DIO mice did not influence body weight gain, nor glucose metabolism. In vitro studies corroborated the in vivo findings in terms of β-cell survival, function, and downstream gene regulation. Taken together, our data suggest that NIK activation is dispensable for the development of diabetes.
Oxidative stress induces meiotic defects of oocytes in a mouse psoriasis model
Authors: Zhang ZhiQin , Tang ShouBin , Jiang YuYing , Long FangYuan , He Fang , Liu Jian , Gu ShouYong , Lu Yan , Yin ZhiQiang 
Published: May 2022

AbstractPsoriasis, an immune-mediated inflammatory disease, is associated with poor pregnancy outcomes. Emerging evidence indicates that these defects are likely attributed to compromised oocyte competence. Nevertheless, little is known about the underlying associated mechanisms between psoriasis and poor oocyte quality. In this study, we construct an imiquimod-induced chronic psoriasis-like mouse model to review the effects of psoriasis on oocyte quality. We discover that oocytes from psoriasis-like mice display spindle/chromosome disorganization, kinetochore-microtubule mis-attachment, and aneuploidy. Importantly, our results show that melatonin supplement in vitro and in vivo not only increases the rate of matured oocytes but also significantly attenuates oxidative stress and meiotic defects by restoring mitochondrial function in oocytes from psoriasis-like mice. Altogether, our data uncover the adverse effects of psoriasis symptoms on oocytes, and melatonin supplement ameliorates oxidative stress and meiotic defects of oocytes from psoriatic mice.
Structural transition and re-emergence of iron's total electron spin in (Mg,Fe)O at ultrahigh pressure
Authors: Hsu Han , Umemoto Koichiro 
Published: May 2022

AbstractFe-bearing MgO [(Mg1−xFex)O] is considered a major constituent of terrestrial exoplanets. Crystallizing in the B1 structure in the Earth’s lower mantle, (Mg1−xFex)O undergoes a high-spin (S = 2) to low-spin (S = 0) transition at ∼45 GPa, accompanied by anomalous changes of this mineral’s physical properties, while the intermediate-spin (S = 1) state has not been observed. In this work, we investigate (Mg1−xFex)O (x ≤ 0.25) up to 1.8 TPa via first-principles calculations. Our calculations indicate that (Mg1−xFex)O undergoes a simultaneous structural and spin transition at ∼0.6 TPa, from the B1 phase low-spin state to the B2 phase intermediate-spin state, with Fe’s total electron spin S re-emerging from 0 to 1 at ultrahigh pressure. Upon further compression, an intermediate-to-low spin transition occurs in the B2 phase. Depending on the Fe concentration (x), metal–insulator transition and rhombohedral distortions can also occur in the B2 phase. These results suggest that Fe and spin transition may affect planetary interiors over a vast pressure range.
SARS-CoV-2 transmission and impacts of unvaccinated-only screening in populations of mixed vaccination status
Authors: Bubar Kate M. , Middleton Casey E. , Bjorkman Kristen K. , Parker Roy , Larremore Daniel B. 
Published: May 2022

AbstractScreening programs that test only the unvaccinated population have been proposed and implemented to mitigate SARS-CoV-2 spread, implicitly assuming that the unvaccinated population drives transmission. To evaluate this premise and quantify the impact of unvaccinated-only screening programs, we introduce a model for SARS-CoV-2 transmission through which we explore a range of transmission rates, vaccine effectiveness scenarios, rates of prior infection, and screening programs. We find that, as vaccination rates increase, the proportion of transmission driven by the unvaccinated population decreases, such that most community spread is driven by vaccine-breakthrough infections once vaccine coverage exceeds 55% (omicron) or 80% (delta), points which shift lower as vaccine effectiveness wanes. Thus, we show that as vaccination rates increase, the transmission reductions associated with unvaccinated-only screening decline, identifying three distinct categories of impact on infections and hospitalizations. More broadly, these results demonstrate that effective unvaccinated-only screening depends on population immunity, vaccination rates, and variant.
Mitochondrial calcium uniporter stabilization preserves energetic homeostasis during Complex I impairment
Authors: Balderas Enrique , Eberhardt David R. , Lee Sandra , Pleinis John M. , Sommakia Salah , Balynas Anthony M. , Yin Xue , Parker Mitchell C. , Maguire Colin T. , Cho Scott , Szulik Marta W. , Bakhtina Anna , Bia Ryan D. , Friederich Marisa W. , Locke Timothy M. , Van Hove Johan L. K. , Drakos Stavros G. , Sancak Yasemin , Tristani-Firouzi Martin , Franklin Sarah , Rodan Aylin R. , Chaudhuri Dipayan 
Published: May 2022

AbstractCalcium entering mitochondria potently stimulates ATP synthesis. Increases in calcium preserve energy synthesis in cardiomyopathies caused by mitochondrial dysfunction, and occur due to enhanced activity of the mitochondrial calcium uniporter channel. The signaling mechanism that mediates this compensatory increase remains unknown. Here, we find that increases in the uniporter are due to impairment in Complex I of the electron transport chain. In normal physiology, Complex I promotes uniporter degradation via an interaction with the uniporter pore-forming subunit, a process we term Complex I-induced protein turnover. When Complex I dysfunction ensues, contact with the uniporter is inhibited, preventing degradation, and leading to a build-up in functional channels. Preventing uniporter activity leads to early demise in Complex I-deficient animals. Conversely, enhancing uniporter stability rescues survival and function in Complex I deficiency. Taken together, our data identify a fundamental pathway producing compensatory increases in calcium influx during Complex I impairment.
A truncated anti-CRISPR protein prevents spacer acquisition but not interference
Authors: Philippe Cécile , Morency Carlee , Plante Pier-Luc , Zufferey Edwige , Achigar Rodrigo , Tremblay Denise M. , Rousseau Geneviève M. , Goulet Adeline , Moineau Sylvain 
Published: May 2022

AbstractCRISPR-Cas systems in prokaryotic cells provide an adaptive immunity against invading nucleic acids. For example, phage infection leads to addition of new immunity (spacer acquisition) and DNA cleavage (interference) in the bacterial model species Streptococcus thermophilus, which primarily relies on Cas9-containing CRISPR-Cas systems. Phages can counteract this defense system through mutations in the targeted protospacers or by encoding anti-CRISPR proteins (ACRs) that block Cas9 interference activity. Here, we show that S. thermophilus can block ACR-containing phages when the CRISPR immunity specifically targets the acr gene. This in turn selects for phage mutants carrying a deletion within the acr gene. Remarkably, a truncated acrIIA allele, found in a wild-type virulent streptococcal phage, does not block the interference activity of Cas9 but still prevents the acquisition of new immunities, thereby providing an example of an ACR specifically inhibiting spacer acquisition.
Modulation of biological motion perception in humans by gravity
Authors: Wang Ying , Zhang Xue , Wang Chunhui , Huang Weifen , Xu Qian , Liu Dong , Zhou Wen , Chen Shanguang , Jiang Yi 
Published: May 2022

AbstractThe human visual perceptual system is highly sensitive to biological motion (BM) but less sensitive to its inverted counterpart. This perceptual inversion effect may stem from our selective sensitivity to gravity-constrained life motion signals and confer an adaptive advantage to creatures living on Earth. However, to what extent and how such selective sensitivity is shaped by the Earth’s gravitational field is heretofore unexplored. Taking advantage of a spaceflight experiment and its ground-based analog via 6° head-down tilt bed rest (HDTBR), we show that prolonged microgravity/HDTBR reduces the inversion effect in BM perception. No such change occurs for face perception, highlighting the particular role of gravity in regulating kinematic motion analysis. Moreover, the reduced BM inversion effect is associated with attenuated orientation-dependent neural responses to BM rather than general motion cues and correlated with strengthened functional connectivity between cortical regions dedicated to visual BM processing (i.e., pSTS) and vestibular gravity estimation (i.e., insula). These findings suggest that the neural computation of gravity may act as an embodied constraint, presumably implemented through visuo-vestibular interaction, to sustain the human brain’s selective tuning to life motion signals.
Poly(GR) and poly(GA) in cerebrospinal fluid as potential biomarkers for C9ORF72-ALS/FTD
Authors: Krishnan Gopinath , Raitcheva Denitza , Bartlett Daniel , Prudencio Mercedes , McKenna-Yasek Diane M. , Douthwright Catherine , Oskarsson Björn E. , Ladha Shafeeq , King Oliver D. , Barmada Sami J. , Miller Timothy M. , Bowser Robert , Watts Jonathan K. , Petrucelli Leonard , Brown Robert H. , Kankel Mark W. , Gao Fen-Biao 
Published: May 2022

AbstractGGGGCC repeat expansion inC9ORF72, which can be translated in both sense and antisense directions into five dipeptide repeat (DPR) proteins, including poly(GP), poly(GR), and poly(GA), is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Here we developed sensitive assays that can detect poly(GA) and poly(GR) in the cerebrospinal fluid (CSF) of patients withC9ORF72mutations. CSF poly(GA) and poly(GR) levels did not correlate with age at disease onset, disease duration, or rate of decline of ALS Functional Rating Scale, and the average levels of these DPR proteins were similar in symptomatic and pre-symptomatic patients withC9ORF72mutations. However, in a patient withC9ORF72-ALS who was treated with antisense oligonucleotide (ASO) targeting the aberrantC9ORF72transcript, CSF poly(GA) and poly(GR) levels decreased approximately 50% within 6 weeks, indicating they may serve as sensitive fluid-based biomarkers in studies directed against the production of GGGGCC repeat RNAs or DPR proteins.