bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative profiling of DNA 6mA at single-base resolution using NAME-seq
Authors: Li Xuwen , Zhang Zijian , Yin Jiekai , Luo Xinlong , Wang Yinsheng , Wu Tao 
Published: November 2022

Abstract
DNA N6-methyladenine (6mA) is prokaryotes' most prevalent type of DNA methylation. Recently, 6mA has been identified in eukaryotic genomes, but the prevalence of 6mA in eukaryotes has been debated due to the limitations of current 6mA genomic profiling and quantification methods. To solve the challenges, we develop a chemical-based sequencing method, Nitrite-assisted Amino MEthylation sequencing (NAME-seq), for genomic profiling and single-base quantification of DNA N6-adenine methylation rate. NAME-seq combines nitrite conversion of 6mA to nitrosylated-6mA (6mA-NO) with Klenow fragment (3'→5' exo−) mediated DNA synthesis to induce the 6mA-to-T transversion specifically. We apply NAME-seq to bacterial and eukaryotic genomes; NAME-seq can accurately map 6mA and quantify the ratio at single-base resolution. Moreover, we show that NAME-seq can be applied with 6mA-MeDIP-seq to map 6mA in the human genome and improve accuracy. In summary, NAME-seq is an efficient method for quantitative 6mA mapping at single-base resolution.
Analysis of the causes of pedestrian-vehicle traffic accidents based on Bayesian networks
Authors: Yang Jianjun , Lai Yu , Shi Hongbo , Chen Yimeng 
Published: November 2022

Abstract
With the continuous improvement of people's living standards, the number of cars has also increased dramatically. While cars are convenient for people to travel, they also lead to increasingly serious traffic safety problems. For this reason, this paper uses the fault tree and Bayesian network methods to conduct an in-depth study on the causes of pedestrian-vehicle traffic accidents from three aspects: people, vehicle, road and the environment. In this paper, the occurrence of pedestrian-vehicle traffic accidents is divided into 29 basic events. The basic events of each of the 381 pedestrian-vehicle traffic accidents were Classified by 0–1. A fault tree model leading to pedestrian-vehicle traffic accidents is established, which is then transformed into a Bayesian network model, and Bayesian network inference, sensitivity analysis is performed with the help of Netica software. Our results suggest that illegal crossing of traffic lanes, speeding, rainy day, slippery road, braking is not timely, visual impairment are the main causes of pedestrian-vehicle traffic accidents. These results can not only provide a reference for transportation technology, but also provide a basis for government legislation.
Effects of simulated gut pH environment on gut bacterial pheromone production of Dendroctonus valens (LeConte)
Authors: Zhao Yu , Koski Tuuli-Marjaana , Li Huiping , Sun Jianghua , Cao Qingjie 
Published: November 2022

Abstract
Bark beetles are economically and ecologically important insect group, which aggregation behavior and thus host colonization success depended on pheromone-mediated communication. For some species, such as for the major invasive forest pest in China, red turpentine beetle (Dendroctonus valens), gut microbiota participates in pheromone production by converting tree monoterpenes into pheromone products. However, how variation in gut microenvironment, such as pH, affects the gut microbial composition, and consequently pheromone production, is unknown. In this study, we fed wild caught D. valens with three different pH mediums (main host diet with natural pH of 4.7; a mildly acidic diet with pH 6 mimicking the beetle’s gut pH; and highly acidic diet with pH 4), and measured their effects on the gut pH, gut bacterial community and production of the main aggregation and anti-aggregation pheromone (verbenone). We further tested the verbenone production capacity of two gut bacterial isolates in different pH environments (pH 6 and 4). Compared to natural state or main host diet, feeding on less acidic diet (pH 6) diluted the gut’s acidity, whereas feeding on highly acidic diet (pH 4) enhanced it. Both changes in gut pH reduced the abundance of dominant bacterial genera, resulting in degrease in verbenone production. Similarly, the highest pheromone conversion rate of the bacterial isolates was observed in pH mimicking the acidity in beetle gut. Taken together, these results indicate that changes in gut pH can affect gut microbiota composition and pheromone production, and may therefore have the potential to affect host colonization behavior.
Atomic-Scale Observation of Stacking Domain Structures in a Semiconducting Van Der Waals Ferrovalley Material VAgP2Se6
Authors: Miao Leixin , Lee Seng Huat , He Jingyang , Gopalan Venkatraman , Mao Zhiqiang , Alem Nasim 
Published: November 2022

Abstract
The van der Waals (vdW) ferrovalley materials show spontaneous valley polarization without external tuning by simultaneously possessing the valley degree of freedom and the broken time-reversal symmetry but are extremely rare. The ferrovalley crystals with spin-orbital coupling are predicted to generate novel quantum phenomena in two-dimensional thin layers, such as the anomalous valley Hall effect. However, due to the reduced symmetry, a potential issue in vdW ferrovalley materials is their complex domain structures. Here, we report domain structure studies in a semiconducting vdW ferrovalley material VAgP2Se6. Our studies reveal clear evidence of second harmonic generation (SHG) response and magnetic hysteresis loop in the bulk VAgP2Se6 crystals suggesting spontaneously broken inversion and time-reversal symmetry. We image its domain structures directly at atomic resolution using aberration-corrected scanning transmission electron microscopy (AC-STEM). Six variants of domains stacking along the c-axis are observed and can be well understood via group theory analysis. The domain structures of vdW materials can create naturally occurring heterostructures that could alter the electronic structures and may lead to topological interfacial states but have not been explored extensively. Our findings open the possibilities for observing and understanding exotic quantum phenomena in vdW ferrovalley materials by engineering the domain structures.
Comprehensive Analysis Uncovers Prognostic and Immunogenic Characteristics of Cellular Senescence for Gliomas
Authors: Sun Zhaohui , Wang Zerong , Zheng Xiaonan 
Published: November 2022

Abstract
Background
Cellular senescence is considered to be an important correlate of tumorigenesis and progression, but the correlation between cellular senescence and immune infiltration of tumors remains unclear in glioma. The prognostic value of cellular senescence in gliomas with immune infiltration needs further investigation.
Methods
We obtained data from TCGA for GBM patients and LGG patients, followed by screening these genes by LASSO-COX based on genes associated with cellular senescence obtained from CellAge thereby obtaining survival-related signature genes, followed by KM analysis, ROC analysis, PCA analysis and immunostaining profiles to verify the risk score as a prognostic indicator of independence and plotting bar lines, and exploring the biological pathways associated with the high-risk group by GSEA analysis. The signature was also validated by combining the genetic information obtained from the China Glioma Genome Atlas (CGGA) database for GBM patients and LGG patients.
Results
We constructed a prognostic signature for five cellular senescence-related genes. They were CENPA, IGFBP-5, TNFSF13, PATZ1 & CDK6. The independence of the risk score as a prognostic indicator was validated by KM analysis, ROC analysis, PCA analysis, and immunohistochemical results. The prognosis of glioma patients was established from a plotted nomogram. We then found that the high-risk group was significantly enriched for pathways in the cell cycle, nuclear division regulation, CD40 signalling pathway and p53 signalling pathway by GSEA analysis. ssGSEA results indicated that the high-risk group was associated with tumor-infiltrating immune cells, including MDSCs, macrophages and Tregs.
Conclusions
We analyzed the clinical significance of different risk groups on glioma prognosis and the role in the immune landscape by constructing an independent prognostic signature based on cellular senescence correlation, which may help to develop personalized immunotherapy strategies for oncologists.
Development and validation of a clinical model to estimate the probability of frailty in older emergency patients
Authors: Li Junyu , Wang Guodong , Liu Huizhen , Shang Na , Wang Na , Guo Shu-Bin 
Published: November 2022

Abstract
Objectives
Most instruments to measure frailty are impractical to operationalize in emergency department (ED), due to the busy work of the staff and the acute presentations of the patients. The current work aimed to develop and validate a clinical model by combining clinical data and biomarkers available in the ED to evaluate the probability of frailty among older emergency patients.
Design:
A cross-sectional research.
Setting and participants:
A total of 273 adults aged 65 years and older who visited the ED of a university-affiliated hospital in Beijing, China during the period from January 2021 to September 2021 were included.
Measurements:
Fried's frailty phenotype was employed for assessing frailty. Clinical data and blood biomarkers were collected within 24 hours of admission. Clinical data included demographic characteristics, chronic diseases, main causes of admission and nutrition risk screening (NRS) 2002 score. Blood biomarkers included hallmarks of inflammation, nutrition and metabolism, coagulation and other biomarkers such as alanine transaminase (ALT), aspartate transaminase (AST), N-terminal pro-brain natriuretic peptide (NT-pro-BNP), and creatinine.
Results
Totally 151 patients were frail. Eight variables were included in the diagnosis model: nutritional risk, prior ischemic heart disease (IHD), age > 77 years, ALT ≤ 9.2 U/L, albumin ≤ 31.9 g/L, homocysteine > 18 umol/L, 25-OH vitamin D ≤ 10.89 ng/mL, and NT-pro-BNP > 2070 ng/L. Through the area under receiver operating characteristics curve (AUC) (0.855), leave-one-out cross-validation (AUC = 0.828), as well as Hosmer-Lemeshow test (P = 0.615), the validity of the diagnosis model was verified. The clinical diagnosis model was converted into a scale score with a total score of 10. The AUC of the diagnostic score for predicting frailty was 0.851 (95% confidence interval: 0.806–0.895, P< 0.001) and the cutoff value was 4.5 points with the Youden index of 0.579. Following adjustment for gender and main causes of admission, the diagnostic score was an independent predictor for 28-day mortality, with a hazard ratio of 1.486 and a 95% confidence interval of 1.224–1.804 (P < 0.001).
Conclusion
In the current work, a clinical diagnosis model to estimate the probability of frailty in older emergency patients was developed using clinical data and biomarkers generally readily available in the ED. This diagnosis model may facilitate targeted intervention and risk stratification in the EDs.
The Role and Place of Nitroxidergic Regulation of The Endothelial System in the Pathogenesis of Acute Lung Abscess
Authors: Okhunov A.O. , Bobokulova Sh.A. 
Published: November 2022

Abstract
Background. Purulent-destructive lung diseases remain a priority among the causes of generalized infection and death. The key to the progression of infection in acute lung abscesses may be associated with impaired barrier-filtration function of this organ, which is based on endothelial dysfunction.
Methods. The experiments were carried out on 32 Chinchilla rabbits, in which the model of acute lung abscess was reproduced. Investigated in blood samples at the entrance and exit from the lungs, such indicators as nitrates, nitrites, peroxynitrite, NO-synthase and von Willebrand factor.
Conclusion. Nitric oxide produced because of iNOS activation is intended for non-specific protection of the body against a wide range of pathogenic agents, inhibits platelet aggregation and improves local blood circulation. However, these changes do not occur. The main role in this direction is assigned to peroxynitrite, which, due to its pathogenicity, worsens the already process associated with endothelial dysfunction. The nature of the changes in the parameters of the nitroxidergic regulation of the endothelial system in the lungs has a staging: compensated and decompensated. All this is of a natural nature, based on certain relationships between the indicators of the nitroxidergic regulation system.
Experiencia de un Sistema de Universidad Abierta y a Distancia en Medicina Veterinaria
Authors: Tellez Reyes Retana Eduardo Ramon 
Published: November 2022

El objetivo de este podcast es compartir la experiencia y la responsabilidad que tuve para el diseño del Sistema de Universidad Abierta y Educación a Distancia (suayed), tanto en la Facultad de Medicina Veterinaria y Zootecnia (fvmz) como a nivel Universidad; y por qué además creo firmemente que el sistema efectivamente funciona para la capacitación y educación de profesionales que necesita el país.
Analisis Pengaruh Ekspor ke China terhadap pendapatan perkapita dan Penyerapan tenaga kerja di indonesia
Authors: Emilia Emilia , Nurjanah Rahma , Aminah Siti 
Published: November 2022

Penelitian ini bertujuan untuk menganalisis perkembangan Nilai Ekspor ke China ,Pendapatan perkapita dan Penyerapan tenaga kerja di indonesia, selama periode tahun 1993 sampai tahun 2014 dan menganalisis pengaruh Nilai Ekspor ke China terhadap Pendapatan perkapita dan Penyerapan tenaga kerja di Indonesiaselama periode tahun 1993 sampai 2014 Perkembangan nilai ekspor Indonesia ke china selama periode tahun 1993 sampai 2014 mengalami fluktuasi dari tahun ke tahun dengan tingkat perkembangan rata-rata selama periode tersebut adalah 15.30% sedangkan rata-rata nilai ekspor selama periode tersebut adalah 8.047,22 Juta US dollar,dalam kurun periode tersebut perkembangan ekspor tertinggi terjadi pada tahun 2011 yakni sebesar 46,18 dan tingkat perkembangan terendah atau mengalami penurunan tertinggi pada periode tersebut terjadi pada tahun 2014 yakni sebesar-22,10%. Selama periode tahun 1993 sampai 2014 nilai rata-rata pendapatan perkapita Indonesia adalah 15.058,04 ribu rupiah dan nilai rata-rata perkembangannya selama periode tersebut adalah 16,61%. selama periode tahun 1993 sampai tahun 2014 rata-rata jumlah tenaga yang bekerja di Indonesia adalah 95.482,95 dalam ribuan orang dengan rata-rata tingkat pertumbuhan adalah 1,79% dengan pertumbuhan tertinggi terjadi pada tahun 1996 sebesar 6,98% dan pertumbuhan terendah terjadi pada tahun 1995 sebesar -2,35%. Hasil Analisis regresi menunjukkan bahwa nilai ekspor ke china berpengaruh positif dan signifikan terhadap pendapatan perkapita dan penyerapan tenaga kerja di Indonesia

Kata Kunci :Nilai Ekspor ke china, Pendapatan perkapita dan Tenaga Kerja
Faktor-faktor yang mempengaruhi ketimpangan pendapatan melalui pertumbuhan ekonomi di Provinsi Jambi
Authors: Damanik Anggiat Mugabe , Zulgani Zulgani , Rosmeli Rosmeli 
Published: November 2022

This study aims to analyze the influence directly or indirectly the number of working population and investment to income inequality through economic growth in Jambi Province. The data used in this study is secondary data sourced from the Office of the Central Bureau of Statistics of Jambi Province. For data analysis tools using path analysis. Economic growth is a variable of mediation in the indirect effect of the working population and investment in income inequality. Based on the results of the analysis, it is known that (1) the number of working population has the insignificant effect on economic growth, investment has the positive and significant effect on economic growth. (2) The number of people working on a positive and significant impact on income inequality, otherwise investment, and economic growth has insignificant effect on income inequality.Keywords: Inequality of Income, The Number of Working Population, Investment, Economic Growth.
AbstrakPenelitian ini bertujuan untuk menganalisis pengaruh secara langsung maupun tidak langsung jumlah penduduk yang bekerja dan investasi terhadap ketimpangan pendapatan melalui pertumbuhan ekonomi di Provinsi Jambi. Data yang digunakan dalam penelitian ini adalah data sekunder yang bersumber dari kantor Badan Pusat Statistik Provinsi Jambi.Untuk alat analisis data menggunakan analisis jalur. Pertumbuhan ekonomi merupakan variabel mediasi dalam pengaruh tidak langsung jumlah penduduk yang bekerja dan investasi terhadap ketimpangan pendapatan. Berdasarkan hasil analisis dietahui bahwa (1) Jumlah penduduk yang bekerja tidak berpengaruh signifikan terhadap pertumbuhan ekonomi, Investasi berpengaruh positif dan signifikan terhaadap pertumbuhan ekonomi. (2) Jumlah penduduk yang bekerja berpengaruh positif dan signifikan terhadap ketimpangan pendapatan, sedangkan investasi dan pertumbuhan ekonomi tidak berpengaruh signifikan terhadap ketimpangan pendapatan. Kata kunci : Ketimpangan Pendapatan, Jumlah Penduduk yang Bekerja, Investasi, Pertumbuhan Ekonomi.
Efektivitas Layanan Informasi Menggunakan Pendekatan Islami Dalam Mengurangi Prokrastinasi Akademik Mahasiswa
Authors: Maharani Reizki 
Published: November 2022

Tujuan artikel ini adalah untuk melihat efektivitas layanan informasi menggunakan pendekatan islami untuk mengurangi prokrastinasi akademik pada mahasiswa. Kurangnya kemampuan mahasiswa dalam menyesuaikan diri di perguruan tinggi menyebabkan adanya mahasiswa yang mengalami permasalahan dibidang akademik. Salah satu permasalahan yang terjadi, khususnya dibidang akademik yaitu perilaku menunda-menunda pada mahasiswa. Kecenderungan mahasiswa untuk melakukan penundaan kegiatan dibidang akademik seperti belajar dan mengerjakan tugas menjadi salah satu penyebab terjadinya fenomena seperti meng copy paste tugas dari internet, mengambil tugas senior, dan tidak mengumpulkan tugas dikalangang mahasiswa.di dalam ajaran islam, perilaku menunda-nunda ini lebih dikenal dengan istilah dalam islam, kebiasaan menunda-nunda ini merupakan salah satu kebiasaan yang tidak disukai Allah SWT. Salah satu upaya yang dapat dilakukan untuk mengurangi prokrastinasi akademik pada mahasiwa yakni dengan memberikan layanan informasi menggunakan pendekatan islami berkaitan dengan prokrastinasi akademik kepada mahasiswa. Hasil penelitian yang telah dilakukan diketahui bahwa layanan informasi menggunakan pendekatan islami efektif dalam mengurangi prokrastinasi akademik mahasiwa.
HUBUNGAN ANTARA PENYESUAIAN DIRI SUAMI ISTRI DENGAN KEHARMONISAN KELUARGA DI DESA TANJUNG PERANAP
Authors: Herwin Herwin , Nurjanis Nurjanis 
Published: November 2022

Penelitian ini dilatar belakangi Oleh pasangan suami istri yang telah menikah yang dari awal memiliki latar belakang yang berbeda, agar hubungan pasangan suami istri dan hubungannya dengan keluarga baik dan selalu harmonis maka diperlukannya suatu hubungan yang baik pula antara hubungan suami isrti dan keluarga yang baik. Adapun masalah dari penelitian ini adalah apakah ada hubungan atau kaitannya antara hubungan penyesuaian diri suami istri dengan keharmonisan keluarga di desa tanjung peranap kecamatan tebing tinggi barat kabupaten kepulauan meranti dan adapun tujuan dilakukan penelitian ini yaitu mengetahui hubungan penyesuaian diri suami istri dengan keharmonisan keluarga di desa Tanjung Peranap Kecamatan Tebing Tinggi Barat Kabupaten Kepulauan Meranti. Adapun populasi pada penelitian ini adalah 214 ( dua ratus empat belas ) orang dan Total sampel berjumlah 54 ( lima puluh empat ) orang dan objek penelitian ini adalah pasangan suami istri yang usia pernikahannya 2-6 tahun kemudian data tersebut dianalisis dengan mengunakan korelasi product moment. Adapun teknik pengumpulan data dikumpul melalui angket, observasi, dan dokumentasi yang dianalisis secara kuantitatif. Berdasarkan metode di atas bahwa hubungan antara penyesuaian diri suami istri dengan keharmonisan keluarga di desa tanjung peranap kecamatan tebing tinggi barat kabupaten kepulauan meranti. Berdasarkan hasil analisis SPSS 17.0, diperoleh nilai koefisien korelasi sebesar 0,646 dengan tingkat probabilitas sig (2-tailed) adalah 0,000 <0,05 maka Ho ditolak dan Ha diterima. Berdasarkan nilai korelasi yang ada pada tabel menunjukkan hubungan yang kuat dengan hasil koefisien korelasi yang ada pada tabel sebesar 0,60- 0,799 hasil menunjukkan bahwa adanya hubungan antara penyesuaian suami istri dengan keharmonisan keluarga adalah kuat r=0,646 dengan tingkat probabilitas 0,000. Artinya hubungan antara variabel X dan Y signifikan.
KAMPANYE KOMUNIKASI JARINGAN KERJA PENYELAMAT HUTAN RIAU (JIKALAHARI) DALAM PENYELAMATAN HUTAN RIAU PASCA KEBAKARAN HUTAN DAN LAHAN TAHUN 2015
Authors: Pratama Reno Nanda , Sukaesih Atjih 
Published: November 2022

Penelitian ini bertujuan untuk mengetahui bagaimana kampanye yang dilakukan jikalahari untuk melakukan penyelamatan hutan beserta segala komponennya pasca kebakaran hutan dan lahan tahun 2015. Teori yang penulis gunakan adalah teori kampanye model Leon Ostergaard yang mempunyai 3 tahapan untuk mendapatkan hasil kampanye, yakni. Pra kampanye, (identification of problem), kampanye (campaign content, public segmentation), pasca kampanye (evaluated), untuk mencari hasil yang mempengaruhi knowledge, attitudes, skills dan behavior. Metode penelitian menggunakan deskriptif kualitatif dan pengumpulan data dilakukan melalui wawancara, dokumentasi serta observasi partisipasi total. Hasil penelitian adalah jikalahari menggunakan analisis politik, fakta lapangan dalam menjalankan kampanye penyelamatan hutan. Aksi konfrontasi pada pihak berwenang dilakukan secara langsung jika tuntutan tidak dihiraukan.
EFEKTIVITAS PENGGUNAAN WEBSITE PADA HUMAS DI KEMENTERIAN AGAMA PROVINSI SUMATERA UTARA
Authors: Ramadayanti Febby , Sazali Hasan , Peranginangin Indira Fatra Deni 
Published: November 2022

Pada saat ini mau tidak mau seorang Humas harus mengikuti perkembangan zaman tersebut dengan menggunakan sebuah website. Website perusahaan pada kinerja humas akan semakin dipermudah, terutama dalam melakukan kegiatan komunikasi, promosi dan sosialisasi kepada khalayak (publik) baik internal maupun eksternal, sehingga pembentukan citra yang baik juga akan mudah dilakukan. Oleh karena itu adanya website lebih mempermudah siapa pun dan dimana pun mereka yang berada diluar kota atau pun jauh untuk mengakses dan mengetahui informasi mengenai Kementerian Agama Sumatera Utara. Tujuan dari penelitian ini adalah untuk mengetahui bagaimana efektivitas penggunaan website pada Humas di Kementerian Agama Sumatera Utara dalam hal kelebihan dan kendala penggunaannya. Hasil penelitian ini menunjukkan bahwa strategi yang digunakan oleh humas Kementerian Agama Sumatera Utara dalam penggunaan website berjalan dengan baik. Humas Kementerian Agama Sumatera Utara selalu membuat berita yang uptodate yang berisi tentang berita utama dan berita daerah. Kelebihan penggunaan website oleh Kementerian Agama Sumatera Utara antara lain: masyarakat bisa membagi data ataupun informasi kebijakan publik oleh Kementerian Agama Sumatera Utara dan website bisa digunakan untuk pencitraan Kementerian Agama Sumatera Utara.