bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paraburkholderia sp. GD17 improves tomato plant growth and resistance to Botrytis cinerea-induced disease
Authors: Gu Anqi , Zhao Danni , Liu Huaqiang , Fu Wei , Li Guangzhe , Hao Lin 
Published: November 2022

Abstract
Background and aims
The plant growth promoting rhizobacteria have been repeatedly addressed in improving plant growth and resistance against pathogens. This study explored the role of Paraburkholderia sp. GD17 in improving tomato plant growth and resistance to Botrytis cinerea.
Methods
Tomato roots were inoculated with GD17 strain, and then the leaves of well-colonized plants were infected with B. cinerea. Physiological and biochemical parameters, and gene expression were analyzed.
Results
In the absence of B. cinerea, GD17 efficiently improved plant growth, and increased photosynthetic efficiency. In the presence of B. cinerea, GD17-bacterized plants exhibited an enhanced resistance, as indicated by 67% of disease index in non-bacterized plants, while by 24% in bacterized ones. In response to B. cinerea, the defense reaction was reinforced in bacterized plants, as shown by enhanced antioxidative capacity and mitigated oxidative damage, as well as increased PR gene expression in bacterized plants compared with control. Photosynthesis was inhibited by B. cinerea, while it was substantially attenuated in bacterized plants. In the presence of B. cinerea, contents of soluble sugar significantly increased in non-bacterized plants, while it was controlled in bacterized plants. The carbohydrate catabolism-related genes, including starch degradation, photorespiration, and pentose phosphate pathway, generally presented a higher expression in bacterized plants under B. cinerea attack.
Conclusions
GD17 strain improved tomato plant growth by increasing the photosynthetic efficiency. GD17 enhanced plant resistance against B. cinerea-induced disease by increasing defense and alleviating oxidative damage. Additionally, GD17 optimized the trade-off between plant growth and defense by strengthening carbohydrate metabolic regulation.
Novel multiphase loop reactor with improved aeration enables foam-free rhamnolipid production by Pseudomonas putida
Authors: von Campenhausen Maximilian , Demling Philipp , Bongartz Patrick , Scheele Alexander , Tiso Till , Wessling Matthias , Blank Lars M. , Jupke Andreas 
Published: November 2022

Abstract
The novel multiphase loop reactor is a modified airlift reactor with an internal loop enabling continuous in situ liquid-liquid extraction. In this study, the reactor is applied for a microbial production of biosurfactants. The obligate aerobic bacterium Pseudomonas putida KT2440 was engineered for rhamnolipid production. Rhamnolipids are biosurfactants with strong foaming capabilities making cultivations in an aerated stirred tank fermenter challenging. The continuous removal of rhamnolipids via in situ liquid-liquid extraction can remedy this foam challenge, and thereby supports long-term cultivation and production. The initially designed multiphase loop reactor had an oxygen transfer rate, which was too low to meet the oxygen demand of the whole-cell biocatalyst, resulting in inefficient growth and production. A re-design of the sparger via 3D-printing enabled a raise in oxygen supply allowed rhamnolipid production at key performance indicators that matched stirred-tank reactor cultivations, but with the advantage of enabling continuous cultivation in the future. Concluding, we present the successful use of the multiphase loop reactor for rhamnolipid synthesis, highlighting its potential to become a new platform technology for intensified bioprocessing.
Impervious surface Mapping and its spatial-temporal evolution analysis in the Yellow River Delta over the last three decades using Google Earth Engine
Authors: Liu Jiantao , Li Yexiang , Zhang Yan , Feng Quanlong , Shi Tongguang , Zhang Dong , Liu Pudong 
Published: November 2022

Abstract
The unique geographical location of the land-sea transition makes the ecological environment of the Yellow River Delta very fragile and vulnerable to human activities. As one of the characteristics of anthropogenic activities, monitoring the spatiotemporal changes of impervious surface is of great significance to the protection of the ecological environment in the Yellow River Delta (YRD). Based on the Landsat historical images and computing resources provided by Google Earth Engine (GEE), an impervious surface area (ISA) mapping method was developed through combining spectral, texture features and random forest algorithm, and subsequently was applied to generate the spatiotemporal distribution data of ISA of the YRD for 1992, 1998, 2004, 2010, 2016 and 2021. The experimental results demonstrated that the proposed method achieved satisfactory accuracy, with an average overall accuracy of 92.23% and an average Kappa coefficient of 0.9090. Through further time-series analysis of ISA, it found that the area of ISA in the YRD increased from the initial 394.87 km2 to 1081.74 km2 during study periods, and the annual growth rate broke through new highs, ranging from the initial 1.01 km2/year to 67.87 km2/year. According to the research results, urban development activities in the region should be strictly restricted in order to protect the ecological environment of the Yellow River Delta.
Tumor Area Positivity (TAP) score of programmed death-ligand 1 (PD-L1): a novel visual estimation method for combined tumor cell and immune cell scoring
Authors: Liu Chunyan , Fang Fang , Kong Ying , ElGabry Ehab 
Published: November 2022

Abstract
Background
Determination of programmed death-ligand 1 (PD-L1) protein expression level in tumor cells and tumor-associated immune cells is critical for identifying patients eligible for immunotherapy. PD-L1 manual scoring algorithms can generally be divided into two categories: cell counting and visual estimation. Cell counting can be time-consuming and is not in sync with pathology practice, which classically uses a Gestalt approach based on pattern recognition and visual estimation. In this study, we introduce the Tumor Area Positivity (TAP) score, which is a novel, straightforward method for scoring tumor cells and immune cells together using visual estimation.
Methods
To demonstrate the reproducibility of TAP scoring among pathologists, between- and within-reader precision studies were performed both within (internal) and outside (external) of our organization. We also compared the TAP score to the Combined Positive Score (CPS), which is based on cell counting, for concordance and time efficacy.
Results
The average positive agreement, average negative agreement, and overall percent agreement between and within readers were all above 85% for both internal and combined external reader precision studies. TAP score had high concordance rate at 5% cutoff compared with CPS at cutoff 1: positive percent agreement, negative percent agreement, and overall percent agreement were all above 85%.
Conclusions
Our study showed the TAP scoring method to be straightforward, significantly less time-consuming, and highly reproducible with a high concordance rate between TAP score and CPS.
Machine Learning Based Low-Complexity Channel State Information Estimation
Authors: Meng Juan , Wei Ziping , Zhang Yang , Li Bin , Zhao Chenglin 
Published: November 2022

Abstract
In 5G communications, the acquisition of accurate channel state information (CSI) is of great importance to the hybrid beamforming of millimeter wave (mmWave) massive multiple-input multiple-output (MIMO) system. In classical mmWave MIMO channel estimation methods, the exploitation of inherent sparse or low-rank structures has demonstrated to improve the performance. However, most high-accurate CSI estimators incur a high computational complexity and require the prior channel information, which hence present the major challenges in the practical deployment. In this work, we leverage machine learning to design the low-complexity and high-performance channel estimator. To be specific, we first formulate the CSI estimation, in the case of sparse structure, as one classical least absolute shrinkage and selection operator (LASSO) problem. In order to reduce the time complexity of existing compressed sensing (CS) methods, we then approximate the original optimization problem to another one, by imposing the other low-rank constraint that was barely considered by CS. We thus solve this new approximated problem and attain the near-optimal solution of the original problem. One new method excludes any prior channel information, and greatly improves the estimation performance, which only incurs a low time complexity. Simulation results demonstrate the superiority of our proposed method both in the estimation accuracy and time complexity.
Metal uptake from iron ore mine tailings by perennial ryegrass (Lolium perenne L.) is higher after wheat straw than wheat straw biochar amendment
Authors: Sarathchandra Sajeevee S. , Rengel Zed , Solaiman Zakaria M. 
Published: November 2022

Abstract
Mine tailings often contain high concentrations of metals that may lead to serious environmental concerns. Phytoremediation by suitable plant species, coupled with organic amendments, represents an intriguing way of improving the quality of mine tailings. We conducted a glasshouse pot study to assess the impact of biochar derived from wheat straw (BC) and wheat straw (WS) at different application rates (w/w: 0%, 1%, 2%, 5%, 10%) in enhancing physicochemical and biological attributes of iron ore mine tailings and promoting perennial ryegrass (Lolium perenne L.) growth and mediating uptake of metals (Co, Cu, Fe, Mn, Zn, Cr, Ni, Pb) and As. The pH of iron mine tailings (6.62 with 0% amendment) significantly increased pH to 8.01 in BC 10% but decreased to 5.33 in WS 10%. However, electrical conductivity, total carbon, total nitrogen and cation exchange capacity increased significantly with application of either amendment. Improvement in physicochemical parameters of tailings by amending with WS showed a significant increase in plant growth than BC amendment. Shoot accumulation of As and Pb by perennial ryegrass was low (below detection limit), whereas that of Co, Cu, Fe, Mn, Zn, Cr and Ni increased with an increase in both BC and WS application. Microbial biomass carbon and respiration were enhanced more in the WS than in BC treatments. This study suggests wheat straw behaved more efficiently than biochar for this iron ore mine tailings.
Bridge symptoms between parenting styles and proximal psychological risk factors associated with adolescent suicide: a network analysis
Authors: Ou Wenwen , Yang Yumeng , Chen Yafei , Li Yunjing , Yang Siqi , Lu Yimei , Li Liang , Huang Mei , Ma Mohan , Lv Guanyi , Zhao Xiaotian , Qing Yaqi , Ju Yumeng , Zhang Yan 
Published: November 2022

Abstract
Background
Parenting styles and the associated proximal psychological factors are suggested to increase suicidal risks in adolescents. However, how the two risky factors interact and confer risks on adolescent suicide remains unclear. Herein, we used a network approach to investigate their interrelationship and explore whether the network properties predict adolescent suicide.
Methods
Self-report questionnaires were completed by 1,171 students aged 12–16. Network analyses were performed by Gaussian graphical models estimating the adolescent psychosocial network structure of parenting styles and psychological variables including depression, anxiety, affective lability, rumination, and resilience. Furthermore, we re-examined the network by adding a variable of active suicidal thoughts. Moreover, we conducted linear regressions to examine the predictive utility of bridge symptoms for adolescent suicide.
Results
Resilience, Afraid, Rumination, Concentration, and affective lability (Anger) had the highest bridge strengths in the adolescent psychosocial network. Among the identified bridge symptoms, Resilience was negatively correlated with active suicidal thoughts (regularized edge weights = -0.181, bootstrapped 95% CIs: [-0.043, -0.155]), whereas affective lability (from Anxiety to Depression, Anger), Rumination, and Afraid were positively correlated with active suicidal thoughts, with edge weights (bootstrapped 95% CIs) ranging from 0.057 (0.001, 0.112) to 0.081(0.026, 0.136). Regression analysis showed that bridge strength was significantly correlated with active suicidal thoughts (R2 = 0.432, P = 0.001).
Conclusion
Negative parenting styles may drive and maintain active suicidal thoughts by modifying the key proximal psychological variables. Our findings highlight the important role of bridge symptoms, which may serve as potential prevention and intervention targets for adolescent suicide.
EBV-positive intravascular large B-cell lymphoma of the small intestine: a case report and literature review
Authors: Pan Chenglong , Yao Yanfei , Ma xiaoling , Wang Chunyan 
Published: November 2022

Abstract
Background: Intravascular large B-cell lymphoma is a rare lymphoma that causes symptoms in the central nervous system, skin, and bone marrow.
Case presentation: We report a 75-year-old man who was admitted to the hospital with abdominal pain for four hours. Physical examination revealed abdominal pain and mottled skin. Laboratory tests revealed thrombocytopenia and elevated levels of lactate dehydrogenase.Abdominal CT examination showed thickening, edema, and necrosis of the small intestine wall. Surgical excision of the necrotic small bowel revealed numerous small rounds, consistent, atypical lymphocytes in the mesenteric vein. These cells were positive for CD20, CD79a, CD10, and BCL-2, and were positive for Epstein Barr virus-encoded small RNA (EBER) by in situ hybridization. The patient was diagnosed with IVLBCL and died of MODS after a week of hospitalization without chemotherapy.
Conclusions: IVLBCL that occurs in the small intestine and even in the gastrointestinal tract is an exceedingly rare disease with insidious onset, rapid disease progression and poor prognosis. Fully understanding its clinicopathological features is helpful to understand the disease, make an early diagnosis, and prevent the rapid deterioration of the disease.
Arctic sea ice loss drives increasing Arctic wind speeds with combined impact on surface roughness and boundary layer stability
Authors: DuVivier Alice K. , Holland Marika M. , Vavrus Stephen J. , Landrum Laura , Shields Christine A. , Thaker Rudradutt 
Published: November 2022

Abstract
The Arctic is undergoing a pronounced and rapid transformation in response to changing greenhouse gases, including reduction in both sea ice spatial extent and sea ice thickness and projected increase in near-surface winds. Other studies have suggested that the increasing winds may be driven by changing surface roughness and/or stability, but the coupled relationship between the changing winds and changing sea ice in different regions and seasons is not yet well understood. This study disentangles ways in which changing surface conditions affect the changing winds regionally and seasonally. We analyze 50 experiments from the Community Earth System Model version 2 Large Ensemble (CESM2-LE) as well as 5 experiments using CESM2 in which sea ice roughness has been decreased to be equivalent to what it would be over the smoother, open ocean (SMOOTH). We find that with a smoother surface there is a small reduction in the surface momentum flux, leading to faster mean wind speeds and slower mean ice speeds. In SMOOTH, the reduction in surface roughness due to ice loss is negligible, resulting in weaker increasing trends in near-surface winds, particularly in Autumn and Winter. There are no significant coupled impacts of surface roughness change on the sea ice mean state or loss trend. Because both the CESM2-LE and SMOOTH experience statistically indistinguishable sea ice loss trends that impact near-surface atmospheric temperatures, the experiments have very similar changes in atmospheric stability which also influences the near-surface winds. Therefore, in CESM2-LE, the near-surface increasing wind speed trends result from both decreases in surface roughness and reduction in near-surface atmospheric stability that are the result of sea ice loss.
Direct transcatheter aortic valve implantation (TAVI) decreases silent cerebral infarction when compared to routine balloon valvuloplasty
Authors: ARSLAN Uğur , Erdoğan Güney , Yenerçağ Mustafa , Aksan Gökhan , Uçar Melisa , Görgün Selim , Akpınar Çetin Kürşat , Öztürk Onur , Yontar Osman Can , Karagöz Ahmet 
Published: November 2022

Abstract
Purpose: Silent cerebral infarctions (SCI), as determined by neuron-specific enolase (NSE) elevations, may develop after the transcatheter aortic valve implantation (TAVI) procedure. Our aim in this study was to compare the SCI rates between patients who underwent routine pre-dilatation balloon aortic valvuloplasty (pre-BAV) and patients who underwent direct TAVI without pre-BAV.
Methods: A total of 139 consecutive patients who underwent TAVI in a single center using the self-expandable Evolut-R valve (Medtronic, Minneapolis, Minnesota, USA) were included in the study. The first 70 patients were included in the pre-BAV group, and the last 69 patients were included in the direct TAVI group. SCI was detected by serum NSE measurements performed at baseline and 12 h after the TAVI. New NSE elevations > 12 ng/mL after the procedure were counted as SCI. In addition, SCI was scanned by MRI (magnetic resonance imaging) in eligible patients.
Results: TAVI procedure was successful in all of the study population. Post-dilatation rates were higher in the direct TAVI group. Post-TAVI NSE positivity (SCI) was higher in the routine pre-BAV group (55(78.6%) vs. 43(62.3%) patients, p=0.036) and NSE levels were also higher in this group (26.8 ± 15.0 vs. 20.5 ± 14.8 ng/ml, p=0.015). SCI with MRI was found to be significantly higher in the pre-BAV group than direct TAVI group (39(55.1%) vs. 31(44.9%) patients). The presence of atrial fibrillation and diabetes mellitus (DM), total cusp calcification volume, calcification at arcus aorta, routine pre-BAV and failure at first try of the prosthetic valve implantation were significantly higher in SCI (+) group. In the multivariate analysis, presence of DM, total cusp calcification volume, calcification at arcus aorta, routine pre-BAV and failure at first try of the prosthetic valve implantation were significantly associated with new SCI development.
Conclusions: Direct TAVI procedure without pre-dilation seems to be an effective method and avoidance of pre-dilation decreases the risk of SCI development in patients undergoing TAVI with a self-expandable valve.
The Electrostatic and Non-Electrostatic Interaction Affect on Acidity Constants of Bromocresol Purple in Aqueous Ethanolic Media
Authors: Hemdan Sokaina Saad , Gebali Asma M. AL , Ali Fatma Khairallah 
Published: November 2022

Abstract

This work aimed to the study effect of ethanol ratios on the acidity constants of bromocresol purple. The acidity constants of BCP were determined in binary mixtures of water with ethanol containing 0, 30, 40, 50, 60, and 70% (v/v) using spectrophotometric methods. The results indicate the dependence of acidity constants on the ratio of ethanol. The values of logKa1 and logKa2 were correlated with the macroscopic (relative permittivity, ε) and microscopic Kamlet-Taft parameters (α, β, and π*) of binary mixtures. The influence of solvent on the acidity constants was analyzed in terms of the KAT parameters in order to study the solvent-solute interaction. Multiple linear regression was used to find the contribution of the microscopic parameters containing α (acidity), π* (dipolarity/polarizability), and β (basicity). It was found that α and β were the most predominant descriptors. Also, a relationship with reciprocal relative permittivity was obtained based on Born’s model, showing the significance of specific solute-solvent interactions. Therefore, the hydrogen bonding interactions between solute and solvent components are mainly responsible for the change in acidity constants of bromocresol purple of binary mixtures.
Single versus double blastocyst transfer in first and second frozen-thawed embryo transfer cycle in advance-aged women: a two-center retrospective cohort study
Authors: Zhou Yuxi , Ji Hui , Zhang Mianqiu , Zhang Juanjuan , Li Xin , Zhang Junqiang , Ling Xiufeng , Chen Li , Zhao Chun 
Published: November 2022

Abstract
Background: The present evidence is deficient for the trade-offs between the pros and cons of single blastocyst transfer (SBT)versus double blastocyst transfer (DBT) in frozen-thawed embryo transfer cycles for women in advanced reproductive age, especially in the second cycle. The current study aimed to investigate the impact of transferred blastocyst numbers on pregnancy outcomes in the first and second embryo transfer for women ≥ 35 years.
Methods: This was a retrospective cohort study including 1284 frozen-thawed blastocyst transfer (FBT) cycles from two reproductive centers. We analyzed the pregnancy outcomes after SBT and DBT in the first and second FBT cycles. Moreover, stratified analysis was conducted by maternal age.
Results:In the first FBT cycle, the LBR was higher in DBT group than that in the SBT group [52.3% vs. 33.9%; adjusted odds ratio (aOR), 1.65; 95% confidence interval (CI), 1.26–2.15, P < 0.001]. However, the LBR of the DBT group showed no remarkable difference compared with that of the SBT group in the second cycle of FBT (44.3% vs. 33.3%; aOR, 1.30; 95% CI, 0.81–2.08; P = 0.271). Furthermore, stratified analysis by age showed a higher LBR for the DBT group than the SBT group in patients aged 38–42 years (43.1% vs. 33.9%; aOR, 2.27; 95% CI, 1.05–4.90; P = 0.036).
Conclusions: The present study demonstrated that the SBT regimen is the best choice for both, the first and second frozen-thawed embryo transfer cycles, for women aged 35–37 years. Additionally, the DBT regimen is still recommended to achieve a high LBR in women aged 38–42 years in the second FBT cycle. These findings may be beneficial for deciding the embryo transfer regimens in women of advanced reproductive age.
The finest particles in dust after sanding untreated and thermally modified spruce, oak and meranti wood
Authors: Sydor Maciej , Majka Jerzy , Hanincová Luďka , Kučerka Martin , Kminiak Richard , Krišťák Ľuboš , Pędzik Marta , Rogoziński Tomasz 
Published: November 2022

Abstract

Airborne wood dust causes health and safety hazards in the construction and furniture industry. The research verified whether the thermal modification affects the share of the finest dust particles, smaller than < 10 µm, in the wood dust. The experimental research included nine variants of materials (untreated and thermally modified – in 160°C by 3 h and in 220°C by 3 h – oak, spruce, and meranti wood). The dust was generated with a belt sander (P120 sandpaper, 14.5 m/s belt speed, and 0.65 N/cm2 pressure). The four fractions with particle sizes < 25, 25–80, 80–250, and > 250 µm, were assessed with sieves with aperture sizes of 25, 80, and 250 µm. The content and size distribution of the finest particles in the fractions were measured with laser particle sizer. The results suggest that: (1) both untreated and thermally modified oak wood dust show the higher share of the finest particles in comparison to spruce and meranti wood. (2) Dust from thermally modified oak and meranti has a lower content of harmful particle fractions than dust from untreated wood. The opposite was observed in the case with spruce wood because spruce dust has a low content of fine fractions and its particles have a more irregular elongated shape. (3) The study took into account the extreme temperatures used in the thermal modification of wood (160 and 220°C), then it can be assumed that the two statements mentioned above are valid in all intermediate temperatures.