bluuebunny/crossref_metadata_2025_split huggingface dataset:
Solvent accessibility of E1α and E1β residues with known missense mutations causing pyruvate dehydrogenase complex (PDC) deficiency: Impact on PDC‐E1 structure and function
Authors: Ducich Nicole H. , Mears Jason A. , Bedoyan Jirair K. 
Published: January 2022

AbstractPyruvate dehydrogenase complex deficiency is a major cause of primary lactic acidemia resulting in high morbidity and mortality, with limited therapeutic options. PDHA1 mutations are responsible for >82% of cases. The E1 component of PDC is a symmetric dimer of heterodimers (αβ/α′β′) encoded by PDHA1 and PDHB. We measured solvent accessibility surface area (SASA), utilized nearest‐neighbor analysis, incorporated sequence changes using mutagenesis tool in PyMOL, and performed molecular modeling with SWISS‐MODEL, to investigate the impact of residues with disease‐causing missense variants (DMVs) on E1 structure and function. We reviewed 166 and 13 genetically resolved cases due to PDHA1 and PDHB, respectively, from variant databases. We expanded on 102 E1α and 13 E1β nonduplicate DMVs. DMVs of E1α Arg112‐Arg224 stretch (exons 5‐7) and of E1α Arg residues constituted 40% and 39% of cases, respectively, with invariant Arg349 accounting for 22% of arginine replacements. SASA analysis showed that 86% and 84% of residues with nonduplicate DMVs of E1α and E1β, respectively, are solvent inaccessible (“buried”). Furthermore, 30% of E1α buried residues with DMVs are deleterious through perturbation of subunit‐subunit interface contact (SSIC), with 73% located in the Arg112‐Arg224 stretch. E1α Arg349 represented 74% of buried E1α Arg residues involved in SSIC. Structural perturbations resulting from residue replacements in some matched neighboring pairs of amino acids on different subunits involved in SSIC at 2.9‐4.0 Å interatomic distance apart, exhibit similar clinical phenotype. Collectively, this work provides insight for future target‐based advanced molecular modeling studies, with implications for development of novel therapeutics for specific recurrent DMVs of E1α.
Logotherapy infused family therapy (LIFT): A systematic review of clinical interventions for family counselors
Authors: Dukes Allison T. , Gutierrez Daniel , Goshorn Jeremy R. 
Published: January 2022

AbstractLogotherapy's tenets show considerable therapeutic potential for couple and family therapy. Using the PRISMA systematic review method, authors searched PsycNet, ProQuest, and PubMed in July 2020 for logotherapeutic family interventions. Authors compiled a list of strategies for practitioners and discuss results and their implications for logotherapeutic family interventions.
The relationship between mindfulness and multicultural counseling competence among mental health practitioners
Authors: Ivers Nathaniel N. , Johnson David A. 
Published: January 2022

AbstractWe examined the relationship between dispositional mindfulness, mindfulness practices, and multicultural counseling competence (MCC) among mental health practitioners. Mindfulness Observe and Nonjudge related positively to MCC Knowledge and MCC Awareness. Nonreact and Act Aware were negatively associated with MCC Awareness. Frequency of mindfulness practices related negatively to MCC Awareness.
Astaxanthin improves the shelf‐life of tilapia fillets stored under refrigeration
Authors: Aracati Mayumi Fernanda , Rodrigues Leticia Franchin , de Oliveira Susana Luporini , Rodrigues Romário Alves , Conde Gabriel , Cavalcanti Erika Nayara Freire , Borba Hirasilva , Charlie‐Silva Ives , Fernandes Dayanne Carla , Eto Silas Fernandes , de Andrade Belo Marco Antonio 
Published: January 2022

AbstractBACKGROUNDAstaxanthin, classified as a xanthophyll, has antioxidant properties about 500 times greater than α‐tocopherols and ten times greater than β‐carotenes. Based on the antioxidant activity of this carotenoid, this study aimed to evaluate the shelf‐life of tilapia fillets (Oreochromis niloticus) fed with astaxanthin, by determining the microbiological quality (colimetry, counts of mesophilic and psychrotrophic microorganisms), physicochemical analyses (colorimetry, pH, thiobarbituric acid reactive substances (TBARS)) and sensory analysis.RESULTSTilapia supplemented with astaxanthin presented a reduction in the counts of microorganisms (mesophiles and psychrotrophics) and lower lipid oxidation index (TBARS), when compared to fillets of control fish. Colorimetric changes of fillet degradation were observed, associated with increased pH during storage, as well as loss of brightness and texture in addition to worsening of appearance and odor. These deteriorating changes were minimized using astaxanthin.CONCLUSIONOur results demonstrate the beneficial performance of astaxanthin in the shelf‐life of tilapia fillets stored under refrigeration. Therefore, dietary supplementation with astaxanthin (100 and 200 mg kg−1 of feed) improves the microbiological and physicochemical quality of tilapia fillets during 50 days of shelf‐life. © 2022 Society of Chemical Industry.
COVID‐19 and the Otolaryngology Residency Match: Rising Incidence of Home Matches
Authors: Bernstein Jeffrey D. , Harmon Matthew , Watson Deborah 
Published: January 2022

Objectives/HypothesisTo quantify the effect of the coronavirus disease 2019 (COVID‐19) pandemic upon the 2020 to 2021 residency match for Otolaryngology‐Head and Neck Surgery (OHNS).Study DesignRetrospective cohort design.MethodsResidency match outcomes for all applicants to our institution during 2020 to 2021 were collected from the National Residency Matching Program including medical school of origin and matched program. Matches were categorized as to home‐program, within‐region, or out‐of‐region and sorted by US geographic region. Matches from the 2020 to 2021 cycle were compared to those from 2019 to 2020, as well as averages and trends from match cycles 2016 to 2020. Statistical analysis included descriptive statistics and chi‐square testing.ResultsDuring 2020 to 2021, there were 436 applicants to our single OHNS program. From 2019–2020 to 2020–2021, the match rate decreased significantly for groups studied, including: All applicants (72.0% [268/372] to 64.7% [282/436]; P = .025); all US MD Senior applicants (76.5% [254/332] to 68.9% [262/380]; P = .024); and US MD Seniors specifically without a home program (77.5% [31/40] to 56.4% [22/39]; P = .046). The match rate for US MD Seniors with a home program did not change significantly (76.4% [223/292] to 70.4% [240/341]; P = .09). From 2019–2020 to 2020–2021, the proportion of US MD seniors who matched to home‐program increased significantly (22.0% [49/223] to 30.0% [72/240]; P = .05).ConclusionThe COVID‐19 pandemic saw high volumes of OHNS applicants with an overall decreased rate of matching compared to previous years. These changes particularly affected applicants without home programs. Home‐program matching increased significantly, likely as a consequence of the limitations placed on in‐person away experiences including interviews. Laryngoscope, 132:1934–1938, 2022
Liming and co‐application of water treatment residuals with biosolids for conditioning sandy soils
Authors: Ribeiro Pablo Lacerda , Bamberg Adilson Luís , Pereira Ivan dos Santos , Monteiro Alex Becker , Potes Mariana da Luz , de Lima Cláudia Liane Rodrigues 
Published: January 2022

AbstractApplication of water treatment residuals (WTR) and biosolids (BS) might ameliorate fragile soils. However, studies integrating impacts of waste preparation methods such as liming and grinding associated with co‐application approaches for soil conditioning are lacking. The present study aimed to evaluate the feasibility of agricultural use of WTR as well as their co‐application with BS to ameliorate a sandy soil through assessing impacts on selected soil quality indicators and on nutrient and potentially toxic element concentration in plant tissue in addition to the biomass yield of annual crops. The following treatments were evaluated: non‐amended soil (control); drying plus grinding and liming WTR; WTR co‐application with BS at 3:1 ratio; and a soil amendment commercially available in Brazil. All treatments were soil incorporated in the 0.0–0.2 m layer at 30 Mg ha−1 dose (dry mass). Selected soil physical, hydraulic, and chemical parameters were determined. Furthermore, nutrients and potentially toxic elements concentration in plant tissue as well as the agronomic performance of maize and ryegrass were measured. The co‐application of WTR and BS increased the soil content of P, Zn, and Cu in the 0.0–0.1 m layer. The concentration of these nutrients also was greater in ryegrass tissue and promoted the biomass yield in 51%. WTR did not increase contaminants and did not decrease the P content in soil and in plant tissue, independently of drying plus grinding and liming. Considering the conditions investigated in our study, WTR/BS co‐application was environmentally safe and effective in ameliorating soil quality.
Impact of crop establishment and residue management on soil properties and productivity in rice‐fallow ecosystems in India
Authors: Kumar Rakesh , Mishra Janki Sharan , Naik Sushanta Kumar , Mondal Surajit , Meena Ram Swaroop , Kumar Saurabh , Dubey Abhishek Kumar , Makarana Govind , Jha Bal Krishna , Mali Santosh Sambhaji , Biswas Ashis Kumar , Choudhary Arbind Kumar , Hans Hansraj , Dubey Rachana , Kumar Santosh , Sundaram Prem Kumar , Raman Rohan Kumar , Monobrullah Mohammad , Kumar Sanjeev , Kumar Ujjwal , Bhatt Bhagwati Prasad 
Published: January 2022

AbstractThe degradation of agricultural land, both chemical and biological, is a big concern all over the world. Crop cultivation practices that are distinctive to each location have negative consequences for agricultural production. Therefore, there is an urgent need for better crop and soil management techniques. This experiment was conducted for 5 years (2016–2020) to identify the best crop establishment‐cum‐residue management (CERM) practices and suitable crops for succession after the rice harvest in rice‐fallow ecosystems of India. Experimentation was conducted in the split‐plot design and each treatment was replicated thrice, having CERM in main‐plot and post‐rainy season/winter crops in sub‐plot. All the CERM treatments had two variants, with crop residue (R+) and without crop residue (R−). Rice yields were markedly higher (19.1%–32.2%) in transplanted puddled rice with residue (TPR‐R+) over conventional‐till direct seeded rice (CTDSR) and zero‐till DSR (ZTDSR) treatments. However, TPR adversely impacted the performance of all the post‐rainy pulses/oilseed crops. Post‐rainy season crop yields under ZTDSR/CTDSR were 14.9%–45.8% higher leading to higher system productivity as compared to TPR. ZTDSR with residue retention showed higher soil aggregation. The practice of ZTDSR increased the soil dehydrogenase activity (46.2%), urease activity (29.8%), soil microbial biomass carbon (65.4), and fluorescein diacetate activity (9.0%) in the crop root zone with 3.3‐ and 4.47‐times higher earthworm population and earthworm biomass, respectively. The rice‐pulse system showed 1.74‐times more earthworm population over rice‐oilseed sequences. Total soil organic carbon (SOC) stock in ZTDSR was 21.8% higher over TPR in 0–45 cm soil profile. Thus, the adoption of ZTDSR practices and diversification of rice‐fallows with pulses crops is recommended for enhancing the crop productivity, increasing SOC stock, and improving soil biological properties in the rice‐fallow ecosystems of India.
Synechococcus silicon accumulation in oligotrophic oceans
Authors: Wei Yuqiu , Sun Jun , Li Liuyang , Cui Zhengguo 
Published: January 2022

AbstractSi accumulation by Synechococcus, small unicellular marine picocyanobacteria that dominate in nutrient‐depleted waters, is significant due to its potential to alter our understanding of the global Si cycle. To date, however, there are only a few studies on Si accumulation in individual Synechococcus cells. To determine the magnitude and variability of Synechococcus cellular Si content in oligotrophic oceans, we analyzed 1348 discrete cells collected from the understudied eastern Indian Ocean (EIO) and western Pacific Ocean (WPO). Synechococcus cellular Si quotas varied widely across both regions, ranging from 0 to 4651 amol Si cell−1. While highly variable in range, average Si quotas were ~ 2.5‐fold lower in Synechococcus cells from the EIO (172 ± 304 amol Si cell−1) than in those from the WPO (438 ± 737 amol Si cell−1). The estimated contributions of Synechococcus to total and picoplankton biogenic Si stocks were < 1–8% and 1–11%, respectively, suggesting that Synechococcus has a small but persistent regional contribution to biogenic Si stocks. Here, we also made a first‐order estimate of the Synechococcus biogenic Si stock for the global ocean, that is, 0.06–0.09 Tmol Si, accounting for 2–3% of the global ocean diatom Si stock. No significant effects of temperature and dissolved inorganic N and P on cellular Si quotas were observed within these oligotrophic ecosystems. However, salinity, ambient dissolved Si, and N/P ratio were closely correlated with the variable Si quotas with different relationships. In addition, Synechococcus cellular Si/P and Si/S ratios associated with physiological processes were significantly influenced by environmental and geographic variables. We thus suggest that the variance in cellular Si quotas may be indirectly driven by ambient variables via some unknown independent or strain‐dependent physiological processes.
Application of artificial neural network to estimate the quality of little auks' potential foraging grounds on Spitsbergen
Authors: Boehnke Rafał , Wichorowski Marcin , Trudnowska Emilia , Balazy Kaja , Jakubas Dariusz , Wold Anette , Wojczulanis‐Jakubas Katarzyna , Falk‐Petersen Stig , Kidawa Dorota , Hop Haakon , Błachowiak‐Samołyk Katarzyna 
Published: January 2022

AbstractThe availability of food for the zooplanktivorous seabirds, such as an endemic High Arctic alcid, the little auk (Alle alle) is essential for its population status and in consequence for fertilizing nutrient‐poor Svalbard tundra. Since the zooplankton composition and concentration vary over time and space on foraging grounds, it is challenging to monitor these changes and it could be facilitated by using original machine‐learning methods. We propose to use supervised artificial neural network (ANN) with back‐propagation algorithm, in which we rank each zooplankton taxonomical category (ZTC) based on its share in biomass of little auks' diet. The highest rank was assigned to the largest and most frequent prey, for example, fifth copepodid stage (CV) of Calanus glacialis (0.75), amphipod Apherusa glacialis (0.17) and females of Calanus hyperboreus (0.12). In order to qualify the potential foraging grounds for little auks, zooplankton samples were collected at sea, in the vicinity of five West Spitsbergen fjords characterized by different oceanographic conditions. Consequently, the southern and middle fjords of Spitsbergen were described as the best foraging grounds, while the lowest quality was designated to the northernmost locations. ANN was validated with independent long‐term monitoring dataset from Hornsund and 78% of stations were classified correctly, which indicates that the presented method is reliable for quick estimation of little auks' foraging grounds qualities. This research proposes a new, automated approach for potential foraging grounds classification and delivers an open‐access application that allows following and predicting changes in an emblematic Arctic predator–prey relationship (little auk‐zooplankton).
Higher Risk, Higher Reward? Self‐Reported Effects of Real‐World Cannabis Use in Parkinson's Disease
Authors: Holden Samantha K. , Domen Christopher H. , Sillau Stefan , Liu Ying , Leehey Maureen A. 
Published: January 2022

AbstractBackgroundDespite limited evidence, people with Parkinson's disease (PD) use cannabis for therapeutic purposes. Given barriers to performing randomized trials, exploring real‐world experiences with cannabis in PD is valuable.ObjectiveInvestigate the frequency and magnitude of symptomatic effects reported with cannabis use in PD.MethodsAn anonymous, 15‐question, web‐based survey was deployed on Fox Insight. Cannabis product types were defined (by relative tetrahydrocannabinol [THC] and cannabidiol [CBD] content) and respondents were asked to reference product labels. Questions focused on use patterns and subjective effects on 36 predefined symptoms (rated −2‐markedly worse to +2‐markedly better).Results1,881 people with PD responded (58.5% men; mean age 66.5; 50.5% <3 years of PD). 73.0% of respondents reported medicinal use, though 30.8% did not inform their doctor. 86.7% knew their type of cannabis product: 54.6% took higher CBD, 30.2% higher THC, and 15.2% took similar amounts of THC and CBD products. Most common use was oral administration, once daily, for less than six months. Frequent improvements were reported for pain, anxiety, agitation, and sleep (>50% of respondents, mean magnitude 1.28–1.51). Dry mouth, dizziness, and cognitive changes were common adverse effects (20.9%–30.8%, mean −1.13 to −1.21). Higher THC users reported more frequent improvements in depression, anxiety, and tremor, and more frequent worsening in dry mouth and bradykinesia than other product types.ConclusionsRespondents with PD reported using more CBD products, via oral administration, with mild subjective benefits primarily for sleep, pain, and mood. Higher THC products may be higher risk/higher reward for PD‐related symptoms.
Altered Cholinergic Innervation in De Novo Parkinson's Disease with and Without Cognitive Impairment
Authors: van der Zee Sygrid , Kanel Prabesh , Gerritsen Marleen J.J. , Boertien Jeffrey M. , Slomp Anne C. , Müller Martijn L.T.M. , Bohnen Nicolaas I. , Spikman Jacoba M. , van Laar Teus 
Published: January 2022

AbstractBackgroundAltered cholinergic innervation plays a putative role in cognitive impairment in Parkinson's disease (PD) at least in advanced stages. Identification of the relationship between cognitive impairment and cholinergic innervation early in the disease will provide better insight into disease prognosis and possible early intervention.ObjectiveThe aim was to assess regional cholinergic innervation status in de novo patients with PD, with and without cognitive impairment.MethodsFifty‐seven newly diagnosed, treatment‐naive, PD patients (32 men, mean age 64.6 ± 8.2 years) and 10 healthy controls (5 men, mean age 54.6 ± 6.0 years) were included. All participants underwent cholinergic [18F]fluoroethoxybenzovesamicol positron emission tomography and detailed neuropsychological assessment. PD patients were classified as either cognitively normal (PD‐NC) or mild cognitive impairment (PD‐MCI). Whole brain voxel‐based group comparisons were performed.ResultsResults show bidirectional cholinergic innervation changes in PD. Both PD‐NC and PD‐MCI groups showed significant cortical cholinergic denervation compared to controls (P < 0.05, false discovery rate corrected), primarily in the posterior cortical regions. Higher‐than‐normal binding was most prominent in PD‐NC in both cortical and subcortical regions, including the cerebellum, cingulate cortex, putamen, gyrus rectus, hippocampus, and amygdala.ConclusionAltered cholinergic innervation is already present in de novo patients with PD. Posterior cortical cholinergic losses were present in all patients independent of cognitive status. Higher‐than‐normal binding in cerebellar, frontal, and subcortical regions in cognitively intact patients may reflect compensatory cholinergic upregulation in early‐stage PD. Limited or failing cholinergic upregulation may play an important role in early, clinically evident cognitive impairment in PD. © 2022 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society
Characterization of the sodium binding state in several food products by23Na nuclear magnetic resonance spectroscopy
Authors: El Sabbagh Nour , Bonny Jean‐Marie , Clerjon Sylvie , Chassain Carine , Pagés Guilhem 
Published: January 2022

AbstractIn food, salt has several key roles including conservative and food perception. For this latter, it is well‐known that the interaction of sodium with the food matrix modifies the consumer perception. It is then critical to characterize these interactions in various real foods. For this purpose, we exploited the information obtained on both single and double quantum23Na nuclear magnetic resonance (NMR) spectroscopies. All salted food samples studied showed strong interactions with the food matrix leading to quadrupolar interactions. However, for some of them, the single quantum analysis did not match the theoretical prediction. This was explained by the presence of another type of sodium population, which did not produce quadrupolar interactions. This finding is of critical importance to perform quantitative magnetic resonance imaging (MRI) and to understand the consumer salty taste perception.
T1D‐weighted ihMT imaging – Part I. Isolation of long‐ and short‐T1D components by T1D‐filtering
Authors: Hertanu Andreea , Soustelle Lucas , Le Troter Arnaud , Buron Julie , Le Priellec Julie , Carvalho Victor N. D. , Cayre Myriam , Durbec Pascale , Varma Gopal , Alsop David C. , Girard Olivier M. , Duhamel Guillaume 
Published: January 2022

AbstractPurposeTo identify T1D‐filtering methods, which can specifically isolate various ranges of T1D components as they may be sensitive to different microstructural properties.MethodsModified Bloch‐Provotorov equations describing a bi‐T1D component biophysical model were used to simulate the inhomogeneous magnetization transfer (ihMT) signal from ihMTRAGE sequences at high RF power and low duty‐cycle with different switching time values for the dual saturation experiment: Δt = 0.0, 0.8, 1.6, and 3.2 ms. Simulations were compared with experimental signals on the brain gray and white matter tissues of healthy mice at 7T.ResultsThe lengthening of Δt created ihMT high‐pass T1D‐filters, which efficiently eliminated the signal from T1D components shorter than 1 ms, while partially attenuating that of longer components (≥ 1 ms). Subtraction of ihMTR images obtained with Δt = 0.0 ms and Δt = 0.8 ms generated a new ihMT band‐pass T1D‐filter isolating short‐T1D components in the 100‐µs to 1‐ms range. Simulated ihMTR values in central nervous system tissues were confirmed experimentally.ConclusionLong‐ and short‐T1D components were successfully isolated with high RF power and low duty‐cycle ihMT filters in the healthy mouse brain. Future studies should investigate the various T1D‐range microstructural correlations in in vivo tissues.