bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of cobalt (III) Schiff base complexes on in-vitro anti-proliferative, oxidative stress and gene expression analysis in HeLa and HepG2 cell lines
Authors: Balakrishnan Gowdhami , R.T.V Vimala , Rajmohamed Mohamed Asik , Balakrishnan Karthiyayini , Subramanian Ambika , Yesaiyan Manojkumar , Thiyagarajan Ramesh , Balamuthu Kadalmani 
Published: April 2022

Abstract
The development of new medicines for the successful treatment of cervical and liver malignancies is critical in order to address the disadvantages of current chemotherapeutics, such as increased resistance. Metal ion-based chemical complexes have recently emerged as a prominent method for cancer therapy. As a result, the study aims to create novel anticancer medicines based on leads acquired through combinatorial chemistry of metal complexes. Cobalt (III) Schiff base trans- [Co(salen)(DA)2](ClO4) (complex 1) and trans-[Co (salophen)(DA)2](ClO4) (complex 2)] where, the salen and salopen were N, N′-bis(salicylidene) ethylenediamine, and N, N′-bis(salicylidene)-1,2- phenylenediamine, DA: dodecylamine) were synthesized as an alternative to the existing drugs, and their cytotoxic effect were evaluated against human cervical (HeLa) and liver cancer cell lines (HepG2) using MTT viability assay, and apoptotic morphological staining which including Acridine Orange/Ethidium Bromide (AO/EB), Hoechst 33528, Annexin V-Cy3 assay, JC-1 staining Comet assay, and reactive oxygen species (ROS) assay. Further the apoptosis was confirmed with immunocytochemistry and real-time reverse transcription-polymerase chain reaction (RT-PCR). The results indicated that cobalt (III) complexes reduced the viability of the HeLa and HepG2 cell lines at their half minimum lethal doses, with cell death induced mainly via apoptotic pathway. Hence, the cobalt complexes 1 and 2 could be act as an effective drug for cervical and liver cancers.
Do biomedical researchers differ in their perceptions of plagiarism across Europe? Findings from an online survey among leading universities
Authors: Yi Nannan , Nemery Benoit , Dierickx Kris 
Published: April 2022

Abstract
Background
Existing research on perceptions of plagiarism and cultural influences mainly focuses on comparisons between the Western World and the Eastern World. However, possible differences within the Western World have hardly been assessed, especially among biomedical academics. The authors compared perceptions of plagiarism among European biomedical researchers who participated in an online survey .
Methods
The present work is based on the data collected in a previous online survey done in 2018 among biomedical researchers working in leading European and Chinese universities. Respondents based in Europe were grouped into three geographical regions (northern Europe, southern Europe and northwestern Europe) and their responses were analyzed using logistic regression analysis with adjustments for demographic factors.
Results
Data were available from 810 respondents (265 northern Europe, 101 southern Europe, 444 northwestern Europe). In addition to their generally similar responses, different perceptions of plagiarism were observed among respondents in the three European regions. The Nordic respondents identified the most types of practices as plagiarism and were the most sensitive to Copying text with crediting the source, without quotation marks [aORN|S 1.64 (95% CI 1.02;2.62), aORN|NW 1.48 (95% CI 1.08;2.02)]. Respondents in northwestern Europe were the least sensitive to unattributed recycling of one’s own dissertation/thesis, by recycling parts of it [aORN|NW 3.12(95% CI 2.24;4.35), aORS|NW 2.22(95% CI 1.38;3.59)] or summarizing it [aORN|NW 2.48(95% CI 1.77;3.48), aORS|NW 1.99(95% CI 1.22;3.22)], but more successful than their southern counterparts in identifying plagiarism of text(aORS|NW 0.18, 95% CI 0.04;0.80), plagiarism of image(aORS|NW 0.26, 95% CI 0.10;0.67), and other practices. The southern respondents were the most sensitive to recycling of previously rejected research proposal [aORN|S 0.34 (95% CI 0.16;0.68), aORS|NW 3.12 (95% CI 1.62;6.03)] and least to plagiarism of image [aORN|S 3.23 (95% CI 1.22;8.60), aORS|NW 0.26 (95% CI 0.10;0.67)], plagiarism of online source [aORN|S 5.21 (95% CI 1.55;17.56), aORS|NW 0.26 (95% CI 0.09;0.74)].
Conclusions
In spite of a general similar response pattern, the present study indicates different perceptions of plagiarism among European biomedical researchers. These intra-European differences should be considered when addressing plagiarism.
Clinicopathological Significance of Cancer Stem Cell-associated ACOT12 Expression in Hepatocellular Carcinoma
Authors: Chen Li , Zou Weijie , Xu Jiachen , Zhang Lei , Jiang Xiaoqing , Ni Caifang 
Published: April 2022

Abstract
The acetyl-CoA pool has been identified using various cell measures in studies related to cell development and mitosis. It is suggested that acetyl-CoA elevates the level of histones at certain qualities of cell development. The relationship between ACOT12 and hepatocellular carcinoma (HCC) is unclear. Here, data on ACOT12 expression were obtained from HPA, GEPIA, TCGA portal, and UALCAN web repositories. Clinicopathological significance of ACOT12 was examined using Kaplan-Meier analysis, OncoLnc, TCGA portal and PROGgeneV2. The association between ACOT12 with cancer stem cells was examined based on GEO datasets GSE111802 and GSE7053. The link between ACOT12 and microRNAs and genes that modulate survival was determined based on Cytoscape, GEPIA, and TargetScan. These analyses revealed that relative to normal liver and paracancerous tissues, ACOT12 expression was downregulated in HCC and favorable liver tumors at both mRNA and protein levels. HCC patients with lower ACOT12 expression exhibited poorer overall and disease-free survival. Additionally, ACOT12 influenced HCC. In particular, low ACOT12 expression was related to the following groups of patients: poorly differentiated HCC, vascular invasion positive, sorafenib untreated, with alcohol consumption and hepatitis infection of HCC. The intersection of differentially expressed genes (DEGs) was examined to assess the underlying mechanism. This prompted the distinguishing proof of five possibly associated genes-GC, KNG1, DNAJC28, CUX2 and KMO. A network containing ACOT12 miR-7, miR-9, and miR-374b was constructed. Taken together, our findings indicate that ACOT12 is decreased in HCC and it might mediate HCC progression via stem cell-related genes.
Long-term physical impairments in survivors of COVID-19-associated ARDS compared to classic ARDS. A Two-Center Study
Authors: Piva Simone , Pozzi Matteo , Bellani Giacomo , Peli Elena , Gitti Nicola , Lucchini Alberto , Bertoni Michele , Goffi Alberto , Marshall John C. , Calza Stefano , Rasulo Francesco A. , Foti Giuseppe , Latronico Nicola 
Published: April 2022

Abstract

We carried out a prospective and retrospective case series study to compare physical outcome performance with an in-person evaluation of 248 COVID-related ARDS (CARDS) patients and 48 classic ARDS patients. At 6 months, patients with classic ARDS compared to CARDS had lower MRCss, handgrip dynamometry, and 6 Minutes Walk Test. Fatigue was more frequently reported by patients with classic ARDS. At 12 months, patients in both groups partially regained physical performances, and the differences in measured variables between classic ARDS and CARDS remained constant over time. Reasons for these differences are likely multifactorial and require further investigations.
Morphometric evaluation of the lacrimal sac fossa in Anatolian population using multidetector computed tomography
Authors: Erdem Huseyin , Pehlivan Umur Anil , Dogan Nazli Totik , Cevik Yigit , Safak Nazire Kilic , Boyan Neslihan , Oguz Ozkan 
Published: April 2022

Abstract
Purpose: To investigate the morphometric characteristics of the lacrimal sac fossa in Anatolian population using multidetector computed tomography (MDCT) images in surgical aspect.Methods: A total of 77 cranial MDCT scans were evaluated. Maximum thickness of the maxillary and lacrimal bones; midpoint thickness, and the distance between anterior and posterior lacrimal crests were measured at the upper, middle, and lower axial planes. The angle between the lacrimal bone and the sagittal plane was measured at the middle plane. The length of the lacrimal sac fossa was calculated.Results: The mean maximum thickness of the maxillary bone at the upper, middle and lower planes were: 3.58 mm, 4.27 mm, and 5.81 mm; the midpoint thickness at each plane were: 2.76 mm, 2.51 mm, and 1.48 mm; the lacrimal bone thickness at each plane were: 0.72 mm, 0.65 mm, and 0.66 mm; the distance between anterior and posterior lacrimal crests at each plane were: 5.96 mm, 8.1 mm, and 4.63 mm; the angle between the lacrimal bone and the sagittal plane in males and females at the middle plane were: 132.73° and 131.46°; the length of the lacrimal sac fossa in males and females were: 15.82 mm and 14.02 mm, respectively.Conclusion: In Anatolian population, bone thicknesses of the lacrimal sac fossa were higher in males, while the angle between the lacrimal bone and the sagittal plane was higher in females. This study indicates that osteotomies can be easily performed in DCR interventions in Anatolian population.
Is there a Clinical Difference in Pediatric Congenital Cholesteatoma according to Age?
Authors: Shin Chol Ho , Kang Woo Seok , Park Hong Ju , Chung Jong Woo , Ahn Joong Ho 
Published: April 2022

Abstract
Objective: We analyzed the clinical characteristics and surgical outcomes of pediatric patients with congenital cholesteatoma of the middle ear according to age.Study Design: From August 1993 to January 2016, we retrospectively reviewed the records of 186 children (136 boys and 50 girls) less than 15 years of age with pathologically confirmed cholesteatoma. We divided the patients into three age groups (~3 years, 3~7 years, 7~15 years) and analyzed their differences in clinical variables. The audiologic outcomes and recurrence rate were also analyzed.Results: There were no significant differences in sex and side distribution among three age groups. However, there were significant differences in chief complaints, location of cholesteatoma in the middle ear, CT findings, operation methods, ossicular erosion, and type of cholesteatoma sac among three groups. In addition, older age, open type cholesteatoma, ossicular erosion, mastoid invasion of cholesteatoma increased the recurrence rate after surgery. However, despite higher preoperative air-bone gap in older children, hearing can be improved enough after proper surgery with ossicular reconstruction. Conclusion: Delayed detection of pediatric cholesteatoma can lead to extensive disease and the need for an aggressive operation, which can result in worse hearing outcomes and an increased recurrence risk. Physicians should familiarize themselves with the clinical characteristics of pediatric cholesteatoma, especially regarding the age distribution of this condition.
Abnormal SPR-mediated photocatalytic enhancement of Ag nanocubes covered by AgCl ultra-thin layer
Authors: Li Yanjiao , Alves Tiago V. , Liu Xiaoyun , Li Mai , Wang Chunrui , Wang Jiale 
Published: April 2022

Abstract
It was theoretically predicted that electric field intensities generated by surface plasmon resonance (SPR) around plasmonic metallic nanostructures could be reduced after covered by wide bandgap semiconductor, thus worsening the SPR-mediated photocatalytic activities. However, we found that the Ag nanocubes (Ag NCs) covered by AgCl demonstrated an abnormal SPR-mediated catalytic enhancement. Herein the PATP-to-DMAB oxidization was used as a model reaction to explore the photocatalytic conversions. It was observed that the Ag NCs covered by AgCl ultra-thin layer (AgCl@Ag NCs) presented better photocatalytic activities than those of individual Ag NCs under 633 nm excitation. To reveal the mechanism behind the superior activities of AgCl@Ag NCs, the PATP-to-DMAB conversions were also carried out in ambient argon (Ar), in which AgCl@Ag NCs demonstrated significantly higher conversions. These results could be interpreted based on the separation of electron-hole pairs in AgCl through SPR effect of Ag, where the electrons in the valence band of AgCl were excited to the empty states of Ag, leaving holes in the semiconductor and resulting in the oxidation of PATP to DMAB. Thus our findings may provide a novel way to enhance SPR-mediated photocatalytic activities on plasmonic metallic nanostructures by the cover of an ultra-thin layer of wide bandgap semiconductor.
OKDNet: Online Knowledge Distillation Network for Single Image Dehazing
Authors: Lan Yunwei , Cui Zhigao , Su Yanzhao , Wang Nian , Li Aihua , Zhang Wei , Li Qinghui , Zhong Xiao 
Published: April 2022

Abstract
Single image dehazing, as a key prerequisite of high-level computer vision tasks, catches more and more attentions. Traditional model-based methods recover haze-free images via atmospheric scattering model, which achieve favorable dehazing effect but endure artifacts, halos, and color distortion. By contrast, recent learning-based methods dehaze by a model-free way, which achieve better color fidelity but tend to acquire under-dehazed results due to lacking of knowledge guiding. To combine these merits, we propose a novel online knowledge distillation network for single image dehazing named OKDNet. Specifically, the proposed OKDNet firstly preprocesses hazy images and acquires abundant shared features by a multiscale network constructed with Attention Guided Residual Dense Blocks (AGRDBs). After that, these features are sent to different branches to generate two preliminary dehazed images via supervision training: one branch acquires dehazed images via the atmospheric scattering model; another branch directly establishes the mapping relationship between hazy images and clear images, which dehazes images by a model-free way. To effectively fuse useful information from these two branches and acquire a better dehazed results, we propose an efficient Feature Aggregation Block (FAB) consisted of multiple parallel convolutions with different receptive. Moreover, we adopt a one-stage knowledge distillation strategy named online knowledge distillation to joint optimization of our OKDNet. The proposed OKDNet achieves superior performance compared with state-of-the-art methods on both synthetic and real-world images with fewer model parameters.
The risk of COVID-19 can be predicted by a nomogram based on the m6A-related gene
Authors: Lu Lingling , Ao Xiulan , Li Yijing , Huang Jiaofeng , Liu Bang , Wu Liqing , Li Dongliang 
Published: April 2022

Abstract
The expression of m6A-related gene and its significance in COVID − 19 patients are still unknown. In the present study, we used the GSE177477 dataset of Gene Expression Omnibus to extract RNA-seq data of patients with COVID-19. The expression levels of 26 m6A-related genes between COVID-19 patients and healthy people were analyzed, thereby gaining 8 different expression genes. COVID-19 patients were classified into two categories, cluster A and cluster B, based on the expression level of the differential gene, which in this study happened to be the asymptomatic and symptomatic groups. In addition, differential expression of immune cells and the correlation with m6A-related genes was also examined in COVID-19 patients. Finally, 5 m6A-related disease characteristic genes, HNRNPA2B1, FTO, YTHDC1, HNRNPC, and WTAP, were screened by random forest and support vector machine algorithm. These five genes were applied to construct a nomogram model for predicting the risk of COVID-19. The calibration curve and decision curve analysis (DCA) show that the nomogram model is effective and has high net benefit. Then clinical impact curve analysis (CICA) revealed that the high-risk patients classified by the nomogram model had a high degree of coincidence with the actual positive patients. In summary, it has been found that m6A-related genes correlate with immune cells. The nomogram model effectively predicts the risk of COVID-19. And m6A-related genes may be associated with the presence or absence of symptoms in COVID-19 patients.
Soil microbes and associated extracellular enzymes play an integral role in nutrient dynamics in acidic and nutrient poor KwaZulu-Natal grassland ecosystem soils.
Authors: Magadlela Anathi , Ndabankulu Khululwa , Egbewale Samson , Tsvuura Zivanai 
Published: April 2022

Abstract
PurposeThis study investigated the effects of long-term phosphorus (P) fertilization and liming on soil microbe diversity and extracellular soil enzyme activities in acidic and nutrient-poor grassland soils in KwaZulu-Natal (KZN). MethodsExperimental soil samples were collected from the Veld Fertilizer Trials (VFT) initiated in 1951 at Ukulinga Research Farm in Pietermaritzburg, South Africa. P fertilizer and liming treatments comprised of superphosphate (336 kg. ha-1) applied once a year and dolomitic lime (2250 kg. ha-1) applied every 5 years (P+L), superphosphate (336 kg. ha-1) applied once a year (+P) and soils with no superphosphate and no dolomitic lime (-P-L). These soils were analyzed for their nutrient concentration, pH, total cation concentrations and exchange acidity, microflora and extracellular enzyme activities. ResultsSoil nutrition, pH, total cations and exchange acidity showed significant differences between the soil treatments. However, nitrogen (N) and organic carbon (C) concentrations showed no significant differences between the soil treatments. P-solubilizing, N-cycling and N-fixing microbial diversity varied among the different soil treatments. Various nutrient cycling bacteria were identified across soil treatments with the most abundant strains in the Pseudomonas genera. β-glucosaminidase enzyme activity was high in acidic soils compared to P-fertilized and limed soils. However, N reductase activity showed no significant differences between the soil treatments. Alkaline phosphatase showed increased activity in P-fertilized soils, whereas acid phosphatase showed increased activity in acidic soils. Conclusion:These experimental results show that soil microbes and soil enzymes play an integral role in nutrient dynamics in acidic and nutrient stressed grassland ecosystem soils.
Trehalose attenuates learning and memory impairments in aged rats via overexpression of miR- 181c
Authors: Shafiei Bentolhoda , Shabani Mohammad , Afgar Ali , Rajizadeh Mohammad Amin , Nazari-Robati Mahdieh 
Published: April 2022

Abstract
MicroRNAs have been recognized as important regulators of the aging process. Trehalose, a natural disaccharide, displays protective effects against neuronal impairment through several mechanisms. However, little is known about the interactive eﬀects of aging and trehalose on behavioral function and underlying miRNA expression patterns in the hippocampus of young and old rats. Male Wistar rats were divided into four groups. Two groups of aged (24 months) and young (4 months) rats were administered 2% trehalose solution for 30 days. Two other groups of aged and young rats received regular tap water. At the end of treatment, rats were assessed for cognitive behavior using the Morris water maze test. The expression level of miR-181c and mir-34c was also measured by qRT-PCR. We found that trehalose treatment reduced learning and memory impairment in old rats compared to control old animals (p<0.05). We also showed that the expression level of miR-181c was significantly increased in trehalose-treated rats (p<0.01). However, analysis of miR-34c expression level indicated no significant difference between trehalose-treated old rats and non-treated old animals (p>0.05). Our results indicated that trehalose treatment improved learning and memory function in aged rats by targeting miR-181c. Therefore, trehalose administration may provide a therapeutic strategy to ameliorate age-associated cognitive impairment.
Moyamoya disease-specific extracellular vesicle-derived microRNAs in the cerebrospinal fluid as revealed by comprehensive expression analysis through microRNA sequencing
Authors: Ota Shinji , Yokoyama Kinya , Kanamori Fumiaki , Mamiya Takashi , Uda Kenji , Araki Yoshio , Wakabayashi Toshihiko , Yoshikawa Kazuhiro , Saito Ryuta 
Published: April 2022

Abstract
We examined the specific changes that occur in the expression levels of extracellular vesicle (EV)-derived microRNAs (miRNAs) in intracranial cerebrospinal fluid (CSF) in moyamoya disease. Patients with arteriosclerotic cerebral ischemia were used as controls to eliminate the effects of cerebral ischemia. Comprehensive expression analysis of miRNAs extracted from EVs by next-generation sequencing (NGS) and validation by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed. Experiments were conducted on eight cases of moyamoya disease and four control cases. In the comprehensive miRNA expression analysis, 153 miRNAs were upregulated and 98 miRNAs were downregulated in moyamoya disease compared to the control (q-value < 0.05 and |log2 fold change| > 1). qRT-PCR performed on the four most valuable miRNAs (hsa-miR-421, hsa-miR-361-5p, hsa-miR-320a, and hsa-miR-29b-3p) associated with vascular lesions among the differentially expressed miRNAs gave the same results as miRNA sequencing. In GO analysis for the target genes, cytoplasmic stress granule was the most significant GO term. This study is the first comprehensive expression analysis of EV-derived miRNAs in the CSF of moyamoya disease patients using NGS. The miRNAs identified here may be related to the etiology and pathophysiology of moyamoya disease.
Case Study on Indigenous Rice Germplasm Conservation in Yuanjiang Based on Stakeholder Theory
Authors: Yi WEN , LIU Ying , Jiwei XU , Rong SUN , Huabin WANG , LIU Qingsong , Hai WANG , RuI XU , Yi LU 
Published: April 2022

Abstract
Native rice, which can grow at higher altitudes, contains high quality genes and genetic characteristics, but its distribution is worrying. Therefore, conservation of native rice is of great significance both from the perspective of germplasm resources conservation and economic value.This paper introduced the stakeholder theory, and based on the results of the three-party evolutionary game model involving local government, enterprises or cooperatives, and farmers, put forward suggestions for wild rice conservation in Yuanjiang County, and provided references for the conservation and sustainable utilization of agricultural biodiversity and genetic resources in food and agriculture in other areas.The results showed that in the short term, the protection of native rice is in urgent need of encouragement, support, publicity and guidance from local governments. There is a close relationship between government subsidies to enterprises and farmers and their enthusiasm for buying and planting native rice.When the subsidy coefficient of government to enterprises is small, enterprises tend to "not purchase". The higher the subsidy coefficient is, the faster the enterprises reach evolutionary stable state, but the faster the government and farmers reach evolutionary stable state slows down.The higher the subsidy coefficient, the faster the farmers, enterprises and cooperatives reach the evolutionary stable state, but the slower the government reaches the evolutionary stable state. When the government's subsidy coefficient to farmers is large, farmers may tend to "do not plant" and the government tends to "do not encourage".Therefore, the government should carefully weigh the subsidies given to enterprises, cooperatives and farmers, and take into account the local financial situation and the status quo of the restoration of native rice planting and in-situ protection.