bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluorescence immunoassay based on phage mimotope for nontoxic detection of Zearalenone in maize
Authors: Zhou Jingming , Wang Xueli , Li Yanghui , Chen Yumei , Liu Yankai , Liu Hongliang , Liang Chao , Zhu Xifang , Qi Yanhua , Wang Aiping 
Published: April 2022

AbstractZearalenone (ZEN) is one of the most common mycotoxin contaminants worldwide. In this study, a phage‐based direct competitive fluorescence immunosorbent assay (P‐dcFLISA) was developed for the detection of ZEN. In this P‐dcFLISA, phage mimotope was used to replace chemically synthesized antigens to improve the safety of experiments. Furthermore, ZnCdSe/ZnS (core/shell) quantum dots (QDs) as fluorescent labeling material replaced chromogenic substrate, which are used in traditional ELISA. The 50% inhibition value (IC50) of P‐dcFLISA was 0.301 ng/ml, which was approximately 12‐fold lower than that of phage‐based indirect competitive enzyme‐linked immunosorbent assay (P‐icELISA). The limit of detection (LOD) of P‐dcFLISA was 0.023 ng/ml and the detection range was 0.060–1.531 ng/ml. In the added recovery assay, recovery rate was 93.18–105.52%, with the coefficient variation (CV) ranging from 8.39% to 10.55%. The establishment of P‐icFLISA not only save time, but also avoid the use of mycotoxins in the detection process, providing a safe method for the detection of mycotoxins. In conclusion, phage‐based fluorescence immunoassay method has great potential application in ZEN detection and agricultural monitoring.
Comparison of coronary revascularization strategies in older adults presenting with acute coronary syndromes
Authors: Shah Ahmed Ijaz , Alabaster Amy , Dontsi Makdine , Rana Jamal S. , Solomon Matthew D. , Krishnaswami Ashok 
Published: April 2022

AbstractBackgroundThe optimal coronary revascularization strategy to maximize the patient‐centered outcome of days alive and out of hospital (DAOH), in multimorbid older (≥65‐years) adults after an acute coronary syndrome (ACS) is incompletely understood.MethodsUsing Kaiser Permanente Northern California Health Plan databases, we identified 3871 patients ≥65‐years presenting with ACS between 1/1/2010–3/1/2018 who underwent coronary revascularization with either coronary artery bypass grafting (CABG, N = 1575) or multivessel percutaneous coronary intervention (PCI, N = 2296). Selection bias was accounted for through propensity score modeling techniques and inverse probability of treatment weighting. Cox proportional hazards models were fit to evaluate the association of revascularization type with outcomes.Primary OutcomesAbsolute DAOH and the relative risk of achieving ≥90%DAOH during three time intervals.Secondary OutcomesAll‐cause mortality, recurrent MI, stroke, rehospitalization, repeat revascularization, and dialysis initiation.ResultsCABG (compared to PCI) was associated with greater absolute number of DAOH, significant after the first year (mean difference at 1‐year: +5.8 days, 95% confidence interval [CI], −1.6 to 13 days; 3‐years: +56 days, 95%CI, +25 to +88 days; 5‐years: + 131 days, 95%CI, +57 to +205 days). The relative risk of achieving ≥90% DAOH significantly favored CABG after the first year (1‐year:1.02, 95%CI, 0.98–1.05; 3‐years:1.06, 95%CI 1.002–1.11, 5‐years:1.12, 95%CI, 1.03–1.22), and was related to lower incidences of all‐cause mortality, repeat revascularization, rehospitalization, incident dialysis, and nonfatal MI with CABG.ConclusionsIn older adults with multivessel or left main coronary artery disease who presented with ACS, CABG, after the first year, was associated with a greater absolute number of DAOH—a geriatric and patient‐centered outcome, compared to PCI. CABG patients also had a higher probability of achieving ≥90%DAOH—with lower all‐cause mortality, recurrent MI, repeat revascularization, new dialysis, and rehospitalization rates. Future randomized trials should study the impact of optimal revascularization strategies on the quality of life of older adults with multimorbidity.
Structural abnormalities of retinal pigment epithelial cells in a light‐inducible, rhodopsin mutant mouse
Authors: Napoli Debora , Strettoi Enrica 
Published: April 2022

AbstractRetinal pigment epithelium (RPE) is a specialized pigmented monolayer dedicated to retinal support and protection. Given the fact that photoreceptor outer segments are the primary energy resource of RPE metabolism, it follows that, when photoreceptor function is compromised, RPE cells are impaired and vice versa. In retinitis pigmentosa (RP), genetic mutations lead to a massive degeneration of photoreceptors but only few studies have addressed systematically the consequences of rod and cone death on RPE cells, which, among others, undergo an abnormal organization of tight junctions (TJs) and a compromised barrier function. The biological mechanisms driving these barrier reorganizations are largely unknown. Studies aimed at addressing general and mutation‐independent changes of the RPE in RP are relevant to reveal new pathogenic mechanisms of this heterogeneous family of diseases and prospectively develop effective therapeutic strategies. Here, we take advantage of a mouse model of RP in which retinal degeneration is spatially restricted to investigate a possible involvement of inflammatory responses in RPE remodeling. By immunostaining for Zona Occludens‐1 (ZO‐1), a structural and functional marker of TJs with pleiotropic functions, we found a partial rescue of TJs organization following local restoration of retinal organization, revealing that TJs structure can recover. Since lack of ZO‐1 from TJs can alter cell density, we counted RPE cells without finding any differences between degenerated and controls animals, indicating preservation of RPE cells. However, we found an increased number of immune cells adhering to the RPE apical surface and a spatial correlation with areas of abnormal ZO‐1 distribution. This suggests that inflammatory processes following photoreceptor degeneration can be responsible for TJs alterations during RP progression and deserve further investigation.
Origins ofantiminingresistance in the life of a grassroots American Indian leader: Prospects for Indigenizing psychobiography
Authors: Gone Joseph P. 
Published: April 2022

AbstractAmerican Indian communities have long been subject to environmental degradation, but successful “grassroots” struggles to end such exploitation are exceedingly rare. How is it that Joseph William Azure—my father and an unsung hero of social change—came to “notice” in 1985 that “our entire [reservation] mountain range was at risk” from destructive gold mining and, in response, to form “a small grassroots traditional society” that created “a lot of local and national publicity for our cause to save” these mountains? To address this question, I adopted and adapted the approaches and methods of psychobiography to trace shifts in his sense of self in response to midlife socialization into Indigenous traditional spirituality. In developing this brief account of his development as a social change agent, I propose that psychobiography may require “Indigenization” if it is to better represent American Indian lives. Specifically, some Indigenous life stories will perhaps require tellings that center on collective endeavors rather than individual ones, reconstruction of life experiences based on comparably limited (material) archives, deeper preservation of the conventions of orality, and curation by close kin rather than by “distanced” analysts.
Longitudinal bidirectional associations between personality and becoming a leader
Authors: Asselmann Eva , Holst Elke , Specht Jule 
Published: April 2022

AbstractObjectiveLeaders differ in their personalities from non‐leaders. However, when do these differences emerge? Are leaders “born to be leaders” or does their personality change in preparation for a leadership role and due to increasing leadership experience?MethodUsing data from the German Socio‐Economic Panel Study, we examined personality differences between leaders (N = 2683 leaders, women: n = 967; 36.04%) and non‐leaders (N = 33,663) as well as personality changes before and after becoming a leader.ResultsAlready in the years before starting a leadership position, leaders‐to‐be were more extraverted, open, emotionally stable, conscientious, and willing to take risks, felt to have greater control, and trusted others more than non‐leaders. Moreover, personality changed in emergent leaders: While approaching a leadership position, leaders‐to‐be (especially men) became gradually more extraverted, open, and willing to take risks and felt to have more control over their life. After becoming a leader, they became less extraverted, less willing to take risks, and less conscientious but gained self‐esteem.ConclusionsOur findings suggest that people are not simply “born to be leaders” but that their personalities change considerably in preparation for a leadership role and due to leadership experience. Some changes are transient, but others last for a long time.
Virtual health care for children: Parental willingness to adopt virtual health‐care technologies
Authors: Lawrence Joanna , Measey Mary‐Anne , Hoq Monsurul , Hiscock Harriet , Rhodes Anthea 
Published: April 2022

AimTo understand parental awareness and appetite for virtual health modalities, including asynchronous communication and remote monitoring. To understand which socio‐demographic characteristics are associated with greater awareness and appetite for virtual health.MethodsNationally representative survey of Australian parents, recruited via an online panel in February 2021 as part of the Royal Children's Hospital National Child Health Poll. Participants were randomly selected from the consumer panel.Results1981 (59.4% response rate) parents completed the survey. 39.9% were aware of virtual health care, defined by digital technology and medical devices to support remote monitoring. Higher levels of awareness were associated with being male, having previous teleconsultation experience, higher education attainment and employment. Most parents reported an appetite for asynchronous communication using an app or website to either provide information prior to a consult (65.9%), provide a photo of a rash or similar (61.7%) or receive medical advice (58.1%). Appetite for wearable devices was similar at 59.9%.ConclusionsWhilst awareness of virtual healthcare was low, appetite exists amongst Australian parents to use technology to support their child's health care. Health‐care models, which incorporate asynchronous communication or symptom monitoring through apps, are likely to be acceptable and offer an accessible and sustainable alternative to traditional face‐to‐face health care.
The multidimensionality of sleep in population‐based samples: a narrative review
Authors: van de Langenberg Sterre C. N. , Kocevska Desana , Luik Annemarie I. 
Published: April 2022

SummaryThe identification of optimal sleep duration recommendations for the general population has long been an important goal on the public health agenda, as both short and long sleep duration have been linked to unfavourable health outcomes. Yet, sleep is more than duration alone and can be described across multiple domains, such as timing, regularity, satisfaction, alertness, and efficiency. We reviewed observational population‐based studies that examined differences in age, sex, and origin across multiple dimensions of sleep. Reviewed literature suggests an increasing prevalence of insomnia symptoms, shorter and less deep sleep in old age. Overall, women report poorer sleep quality than men despite objective measures revealing shorter and more fragmented sleep in men. Minorities generally have poorer quantity and quality of sleep, but multi‐ethnic studies have reported mixed results regarding the subjective experience of sleep. In sum, effects of age, sex and origin differ across sleep dimensions, thereby suggesting that the multidimensionality of sleep and how these different aspects interact should be studied across individuals. Studies should include both self‐reported measures and objective assessments in diverse population‐based samples, as both aspects are important to understand sleep health in the general population. Data‐driven descriptions could provide researchers and clinicians with insights into how well individuals are sleeping and offer concrete targets for promotion of sleep health across the population.
Thromboelastography in obese horses with insulin dysregulation compared to healthy controls
Authors: Lovett Amy L. , Gilliam Lyndi L. , Sykes Benjamin W. , McFarlane Dianne 
Published: April 2022

AbstractBackgroundBoth obesity and metabolic syndrome are associated with hypercoagulability in people, increasing the risk of cardiovascular disease and thromboembolic events. Whether hypercoagulability exists in obese, insulin‐dysregulated horses is unknown.Hypothesis/ObjectivesTo determine if coagulation profiles differ between healthy horses and those with obesity and insulin dysregulation.AnimalsFifteen healthy horses (CON) and 15 obese, insulin‐dysregulated horses (OBID). Individuals were university or client owned.MethodsCase‐control study. Obesity was defined as a body condition score (BCS) ≥7.5/9 (modified Henneke scale). Insulin dysregulation status was assessed by an oral sugar test (OST). Kaolin‐thromboelastography and traditional coagulation variables were compared between groups. The direction and strength of the association between coagulation variables and BCS and OST results were determined using Spearman's correlation.ResultsThromboelastography variables MA (OBID: 69.5 ± 4.5 mm; CON: 64.8 ± 4.3 mm; P = .007) and G‐value (OBID: 11749 ± 2536 dyn/m2; CON: 9319 ± 1650 dyn/m2; P = .004) were higher in OBID compared to CON. Positive correlations between MA and BCS (R = 0.45, P = .01) and serum insulin (T0: R = 0.45, P = .01; T60: R = 0.39, P = .03), and G‐value and BCS (R = 0.46, P = .01), and serum insulin (T0: R = 0.48, P = .007; T60: R = 0.43, P = .02; T90: R = 0.38, P = .04) were present.Conclusions and Clinical ImportanceObese, insulin‐dysregulated horses are hypercoagulable compared to healthy controls.
The impact of COVID‐19 on aquaculture in China and recommended strategies for mitigating the impact
Authors: Yuan Yuan , Miao Weimin , Yuan Xinhua , Dai Yunyun , Yuan Yongming , Gong Yunchong 
Published: April 2022

AbstractWe carried out a preliminary investigation to study the impact of COVID‐19 on aquaculture in China and identify the strategies and measures that have been taken by the Chinese Government. The investigation involved questionnaire surveys designed for all stakeholders along the industrial chain, including grow‐out farmers, seed producers, fish processors, fish traders, and feed companies engaged in the catfish sector in Hubei Province and the tilapia sector in Guangdong Province during the strict period of control and after these control measures were lifted. We also attempted to summarize the government interventions and measures taken by different stakeholders along the value chain to minimize the damage caused by COVID‐19 and support the recovery of different sectors in the aquaculture industry. We found that due to delayed harvesting, fish stocks were held‐up in ponds and normal farming was interrupted. Farmers and traders were more severely impacted by the pandemic than other sectors. Furthermore, a series of strategies and measures are recommended to cope with the pandemic and other similar risks in the future. We expect that this study will provide good evidence for international societies to support the aquaculture industry in minimizing the impact of the pandemic and the rapid recovery of the industry in the post‐pandemic period.
A fibre and phenolic-rich flour from Isabel grape by-products with stimulatory effects on distinct probiotics and beneficial impacts on human colonic microbiota in vitro
Authors: Silva F.A. , de Souza E.L. , Queiroga R.C.R.E. , Voss G.B. , Pintado M.M.E. , Vasconcelos M.A.S. 
Published: April 2022

Abstract
This study evaluated the effects of a fibre and phenolic-rich flour (IGF) prepared from Isabel grape by-products on the growth and metabolism of different probiotics and distinct bacterial populations part of the human intestinal microbiota during an in vitro colonic fermentation. IGF was submitted to simulated gastrointestinal digestion before use in the experiments. IGF favoured the growth of the probiotics Lactobacillus acidophilus La-05, L. casei L-26 and Bifidobacterium lactis Bb-12, with viable counts of &gt;7 log CFU per ml, as well as caused decreases in pH values and increases in organic acid production in the growth medium during 48 h of cultivation. IGF increased the population of beneficial micro-organisms forming the human intestinal microbiota, particularly Lactobacillus spp., decreased the pH values, and increased the lactic acid and short-chain fatty acid (acetic, butyric and propionic acids) production during 24 h of in vitro colonic fermentation. These results indicate the potential prebiotic effects of IGF, which should represent a novel sustainable added-value ingredient with functional properties and gut-health benefits.
A case of chronic inflammatory demyelinating polyneuropathy following COVID‐19 vaccine
Authors: Katada Eiichi , Toyoda Takanari , Yamada Gohei , Morishima Akira , Matsukawa Noriyuki 
Published: April 2022

AbstractWe report a 44‐year‐old woman who presented with bilateral weakness of the hands and distal paresthesia of the arms on the next day of the second COVID‐19 vaccine, and gradually progressed ascending weakness of the arms and legs, and sensory ataxia beyond 2 months. She was diagnosed as a chronic inflammatory demyelinating polyneuropathy (CIDP) following COVID‐19 vaccine on the basis of clinical and electrophysiological findings. This is a first case diagnosed as a CIDP following COVID‐19 vaccine alone.
Guidewire‐assisted placement of water‐perfused esophageal high‐resolution manometry probe when gastric insertion fails: A single‐center experience
Authors: Delbaen Corinne , Gkolfakis Paraskevas , Devière Jacques , Blero Daniel , Louis Hubert 
Published: April 2022

AbstractBackgroundBlind positioning of a high‐resolution manometry (HRM) probe across the esophagogastric junction (EGJ) is not always possible. We report our experience using guidewire‐assisted water‐perfused HRM probe insertion when the EGJ could not be traversed.MethodsRetrospective study analyzing the failure rate of EGJ insertion during HRM, and reporting a series of guidewire‐assisted procedures.Key ResultsAmong 2727 HRM procedures, the failure rate for traversing the EGJ was 2.7% (73 patients). The technique of guidewire‐assisted placement of the HRM probe was used in 25 patients; it was well‐tolerated and successful in all patients. No motility disorder was found in 6 patients. In four patients with previously diagnosed achalasia, achalasia subtype changed to type III in one patient. While a suspected motility disorder at barium esophagram and/or initial imperfect HRM tracing was confirmed in 10 patients, a new motility disorder was diagnosed in five patients using guidewire‐assisted placement of the HRM probe.Conclusions and InferencesIn cases of inability to traverse the EGJ, insertion of a water‐perfused HRM probe using an endoscopically‐placed nasogastric guidewire allows successful EGJ and esophageal peristalsis assessment. Although motility disorders are often suspected using alternative diagnostic modalities, guidewire‐assisted placement of HRM may be helpful for revealing them in patients where alternative diagnostic modalities are either unavailable or inconclusive.
An integrated high‐resolution mapping shows congruent biodiversity patterns of Fagales and Pinales
Authors: Lyu Lisha , Leugger Flurin , Hagen Oskar , Fopp Fabian , Boschman Lydian M. , Strijk Joeri Sergej , Albouy Camille , Karger Dirk N. , Brun Philipp , Wang Zhiheng , Zimmermann Niklaus E. , Pellissier Loïc 
Published: April 2022

Summary

The documentation of biodiversity distribution through species range identification is crucial for macroecology, biogeography, conservation, and restoration. However, for plants, species range maps remain scarce and often inaccurate.

We present a novel approach to map species ranges at a global scale, integrating polygon mapping and species distribution modelling (SDM). We develop a polygon mapping algorithm by considering distances and nestedness of occurrences. We further apply an SDM approach considering multiple modelling algorithms, complexity levels, and pseudo‐absence selections to map the species at a high spatial resolution and intersect it with the generated polygons.

We use this approach to construct range maps for all 1957 species of Fagales and Pinales with data compilated from multiple sources. We construct high‐resolution global species richness maps of these important plant clades, and document diversity hotspots for both clades in southern and south‐western China, Central America, and Borneo. We validate the approach with two representative genera, Quercus and Pinus, using previously published coarser range maps, and find good agreement.

By efficiently producing high‐resolution range maps, our mapping approach offers a new tool in the field of macroecology for studying global species distribution patterns and supporting ongoing conservation efforts.
Mathematics–gender stereotype endorsement influences mathematics anxiety, self‐concept, and performance differently in men and women
Authors: Rossi Serena , Xenidou‐Dervou Iro , Simsek Emine , Artemenko Christina , Daroczy Gabriella , Nuerk Hans‐Christoph , Cipora Krzysztof 
Published: April 2022

AbstractMathematics anxiety (MA) is negatively associated with mathematics performance. Although some aspects, such as mathematics self‐concept (M self‐concept), seem to modulate this association, the underlying mechanism is still unclear. In addition, the false gender stereotype that women are worse than men in mathematics can have a detrimental effect on women. The role that the endorsement of this stereotype (mathematics–gender stereotype (MGS) endorsement) can play may differ between men and women. In this study, we investigated how MA and mathematics self‐concept relate to arithmetic performance when considering one's MGS endorsement and gender in a large sample (n = 923) of university students. Using a structural equation modeling approach, we found that MA and mathematics self‐concept mediated the effect of MGS endorsement in both men and women. For women, MGS endorsement increased their MA level, while in men, it had the opposite effect (albeit weak). Specifically, in men, MGS endorsement influenced the level of the numerical components of MA, but, unlike women, it also positively influenced their mathematics self‐concept. Moreover, men and women perceived the questions included in the considered instruments differently, implying that the scores obtained in these questionnaires may not be directly comparable between genders, which has even broader theoretical and methodological implications for MA research.
In Defense of Constitutivism About Epistemic Normativity
Authors: Horst David 
Published: April 2022

AbstractEpistemic constitutivism(EC ) holds that the nature of believing is such that it gives rise to a standard of correctness and that other epistemic normative notions (e.g., reasons for belief) can be explained in terms of this standard. If defensible, this view promises an attractive and unifying account of epistemic normativity. However,ECfaces a forceful objection: that constitutive standards of correctness are never enough for generating normative reasons. This paper aims to defendECin the face of this objection. I do so in two steps. First, I dispute a crucial assumption underlying the case againstEC: that constitutive standards of correctness in general are ‘reason‐giving’ only if and because there is also apriorreason to comply with them. Second, I outline a strategy of howECcan meet the challenge of explaining what's special about the activity of believing such that, unlike other standard‐governed activities, it is capable of generating normative reasons.