bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term culture of patient-derived cardiac organoids recapitulated Duchenne muscular dystrophy cardiomyopathy and disease progression
Authors: Marini Vittoria , Marino Fabiola , Aliberti Flaminia , Giarratana Nefele , Pozzo Enrico , Duelen Robin , Cortés Calabuig Álvaro , La Rovere Rita , Vervliet Tim , Torella Daniele , Bultynck Geert , Sampaolesi Maurilio , Chai Yoke Chin 
Published: August 2022

Duchenne Muscular Dystrophy (DMD) is an X-linked neuromuscular disease which to date is incurable. The major cause of death is dilated cardiomyopathy however, its pathogenesis is unclear as existing cellular and animal models do not fully recapitulate the human disease phenotypes. In this study, we generated cardiac organoids from patient-derived induced pluripotent stem cells (DMD-COs) and isogenic-corrected controls (DMD-Iso-COs) and studied if DMD-related cardiomyopathy and disease progression occur in the organoids upon long-term culture (up to 93 days). Histological analysis showed that DMD-COs lack initial proliferative capacity, displayed a progressive loss of sarcoglycan localization and high stress in endoplasmic reticulum. Additionally, cardiomyocyte deterioration, fibrosis and aberrant adipogenesis were observed in DMD-COs over time. RNA sequencing analysis confirmed a distinct transcriptomic profile in DMD-COs which was associated with functional enrichment in hypertrophy/dilated cardiomyopathy, arrhythmia, adipogenesis and fibrosis pathways. Moreover, five miRNAs were identified to be crucial in this dysregulated gene network. In conclusion, we generated patient-derived cardiac organoid model that displayed DMD-related cardiomyopathy and disease progression phenotypes in long-term culture. We envision the feasibility to develop a more complex, realistic and reliable in vitro 3D human cardiac-mimics to study DMD-related cardiomyopathies.
Semiconductor quantum dots for photodynamic therapy: Recent advances
Authors: Uprety Bhawna , Abrahamse Heidi 
Published: August 2022

Photodynamic therapy is a promising cancer treatment that induces apoptosis as a result of the interactions between light and a photosensitizing drug. Lately, the emergence of biocompatible nanoparticles has revolutionized the prospects of photodynamic therapy (PDT) in clinical trials. Consequently, a lot of research is now being focused on developing non-toxic, biocompatible nanoparticle-based photosensitizers for effective cancer treatments using PDT. In this regard, semiconducting quantum dots have shown encouraging results. Quantum dots are artificial semiconducting nanocrystals with distinct chemical and physical properties. Their optical properties can be fine-tuned by varying their size, which usually ranges from 1 to 10 nm. They present many advantages over conventional photosensitizers, mainly their emission properties can be manipulated within the near IR region as opposed to the visible region by the former. Consequently, low intensity light can be used to penetrate deeper tissues owing to low scattering in the near IR region. Recently, successful reports on imaging and PDT of cancer using carbon (carbon, graphene based) and metallic (Cd based) based quantum dots are promising. This review aims to summarize the development and the status quo of quantum dots for cancer treatment.
Associations between triglyceride-glucose index and different hypertension subtypes: A population-based study in China
Authors: Cai Qian , Xing Cathleen Y. , Zhu Jiang , Wang Ying , Lu Fanghong , Peng Jie 
Published: August 2022

BackgroundAbnormal glycolipid metabolism plays a crucial role in hypertension. While an elevated triglyceride-glucose (TyG) index has been recognized as a risk factor for developing hypertension, the associations between the TyG index and different hypertension subtypes, namely, isolated systolic hypertension (ISH), isolated diastolic hypertension (IDH), and systolic-diastolic hypertension (SDH), remain unclear. This study was designed to investigate the associations between the TyG index and hypertension subtypes in a general Chinese population.Materials and methodsIn a sample of 16,793 participants from Shandong Province, China, multivariate logistic regression analyses were performed to examine the associations between the TyG index and different hypertension subtypes. Loess smooth curves were fitted to visualize the trends. Stratified analyses were conducted to further assess the potential interactions in the associations between the TyG index and different hypertension subtypes.ResultsA higher TyG index was associated with an increased odds of having IDH (OR = 2.94, 95% CI: 1.66–5.23) and SDH (OR = 1.82, 95% CI: 1.33–2.49), whereas no apparent relationship was observed between TyG index and ISH. With respect to sex, the effect of TyG index on having IDH and SDH was significant in women, but not in men. Participants with lower lipid profiles and glucose levels demonstrated a stronger strength of association between the TyG index and IDH as compared with the TyG index-SDH association. Stratified analysis showed that participants with a higher TyG index were more than 3 times more likely to have IDH and SDH among persons aged 18–42 years. Significant interactions were observed between TyG index and sex, age, and high-density lipoprotein cholesterol (HDL-C) in the SDH group, and a significant interaction was also found between TyG index and body mass index (BMI) in the ISH group.ConclusionTriglyceride-glucose index may potentially serve as a novel indicator for IDH and SDH. Our findings could also inform the development and implementation of targeted screening for hypertension.
Combining electrocardiographic criteria for predicting acute total left main coronary artery occlusion
Authors: Liu Chunwei , Yang Fan , Hu Yuecheng , Zhang Jingxia , Li Ximing , Guo Zhigang , Liu Yin , Cong Hongliang 
Published: August 2022

BackgroundPrediction of left main artery (LM) occlusion may contribute to the administration of early reperfusion. We sought to identify electrocardiographic (ECG) features associated with acute total LM occlusion and explore the relationship between ECG features and collateral circulation.MethodsWe retrospectively studied ECGs in 84 consecutive patients with LM occlusion between January 2001 and April 2022. The ECG findings in these patients were compared with those in 468 consecutive patients with LM subtotal occlusion and non-LM occlusion.ResultsThree main ECG patterns were described according to the characteristics of ST elevation (STE) in LM occlusion: ST-segment elevation myocardial infarction (STEMI), STE in aVR with diffuse ST depression, and STE in both aVR and aVL. These ECG patterns were associated with different collateral filling territories. One-third STEMI in LM occlusion showed STE in the precordial leads including V1, while 2/3 STEMI showed STE in the precordial leads from V2 to V5 without STE in V1. The following ECG characteristics predicted LM occlusion: STE in both aVR and aVL; STE in I, aVL, and V2–V5 without V1; left anterior fascicular block (LAFB); right bundle branch block (RBBB) + LAFB; and prolongation of the QRS interval. The incidences of STE in aVR and STE in aVR and V1 were higher in LM subtotal occlusion than in LM occlusion. The combination of two different STE criteria (STE in aVR and aVL and STE in I, aVL, V2-V5 without V1) predicted LM occlusion with 62% sensitivity and 95% specificity. The combination of the STE criteria and fascicular block criteria (LAFB and LAFB + RBBB) further improved the specificity to 99% but reduced the sensitivity to 39%.ConclusionThe combination of STE criteria predicted LM occlusion with high specificity and moderate sensitivity, and the addition of fascicular block criteria further improved the specificity with some loss of sensitivity.
Deep learning assessment of left ventricular hypertrophy based on electrocardiogram
Authors: Zhao Xiaoli , Huang Guifang , Wu Lin , Wang Min , He Xuemin , Wang Jyun-Rong , Zhou Bin , Liu Yong , Lin Yesheng , Liu Dinghui , Yu Xianguan , Liang Suzhen , Tian Borui , Liu Linxiao , Chen Yanming , Qiu Shuhong , Xie Xujing , Han Lanqing , Qian Xiaoxian 
Published: August 2022

BackgroundCurrent electrocardiogram (ECG) criteria of left ventricular hypertrophy (LVH) have low sensitivity. Deep learning (DL) techniques have been widely used to detect cardiac diseases due to its ability of automatic feature extraction of ECG. However, DL was rarely applied in LVH diagnosis. Our study aimed to construct a DL model for rapid and effective detection of LVH using 12-lead ECG.MethodsWe built a DL model based on convolutional neural network-long short-term memory (CNN-LSTM) to detect LVH using 12-lead ECG. The echocardiogram and ECG of 1,863 patients obtained within 1 week after hospital admission were analyzed. Patients were evenly allocated into 3 sets at 3:1:1 ratio: the training set (n = 1,120), the validation set (n = 371) and the test set 1 (n = 372). In addition, we recruited 453 hospitalized patients into the internal test set 2. Different DL model of each subgroup was developed according to gender and relative wall thickness (RWT).ResultsThe LVH was predicted by the CNN-LSTM model with an area under the curve (AUC) of 0.62 (sensitivity 68%, specificity 57%) in the test set 1, which outperformed Cornell voltage criteria (AUC: 0.57, sensitivity 48%, specificity 72%) and Sokolow-Lyon voltage (AUC: 0.51, sensitivity 14%, specificity 96%). In the internal test set 2, the CNN-LSTM model had a stable performance in predicting LVH with an AUC of 0.59 (sensitivity 65%, specificity 57%). In the subgroup analysis, the CNN-LSTM model predicted LVH by 12-lead ECG with an AUC of 0.66 (sensitivity 72%, specificity 60%) for male patients, which performed better than that for female patients (AUC: 0.59, sensitivity 50%, specificity 71%).ConclusionOur study established a CNN-LSTM model to diagnose LVH by 12-lead ECG with higher sensitivity than current ECG diagnostic criteria. This CNN-LSTM model may be a simple and effective screening tool of LVH.
The clinical outcomes of reni-angiotensin system inhibitors for patients after transcatheter aortic valve replacement: A systematic review and meta-analysis
Authors: Wang Shuai , Lin Xiaoxiao , Guan Yihong , Huang Jinyu 
Published: August 2022

AimsThe objective of our systematic reviews and meta-analysis is to evaluate the clinical outcomes of RAS inhibitors for patients after TAVR.Methods and resultsWe performed a comprehensive search for Embase, Pubmed, and Cochrane databases from inception to May 1, 2022. The analysis of all outcomes was performed using the random-effects model. In total, 7 articles with a total of 32,585 patients (RAS inhibitor,N= 14,871; Controls,N= 17,714) were included in our study. There was a significantly lower rates of all-cause mortality (RR = 0.76, 95%Cl = 0.68 to 0.86,P&lt; 0.01), cardiovascular death (RR = 0.66, 95%Cl = 0.59–0.74,P&lt; 0.01) and HF readmission (RR = 0.87, 95%Cl = 0.80–0.94,P&lt; 0.01) in patients with RAS inhibitors compared with controls. Patients with RAS inhibitors also had lower rates of all-cause mortality (RR = 0.82, 95%Cl = 0.76–0.89,P&lt; 0.01) and cardiovascular death (RR = 0.73, 95%Cl, 0.62–0.85,P&lt; 0.01) after propensity matching.ConclusionsIn conclusion, our systematic reviews and meta-analysis demonstrated that RAS inhibitors could improve the clinical outcomes for patients after TAVR. Further large and high-quality trials should be conducted to support the use of RAS inhibitors for patients after TAVR.
Redistribution of adipose tissue is associated with left atrial remodeling and dysfunction in patients with atrial fibrillation
Authors: Chen Qian , Chen Xiuzhen , Wang Jiafu , Zhong Junlin , Zhang Hui , Wu Bingyuan , Zheng Zhenda , Xie Xujing , Zhu Jieming , Tang Xixiang , Li Suhua 
Published: August 2022

ObjectiveAdipose tissue is recognized as a crucial regulator of atrial fibrillation (AF). However, the effect of epicardial adipose tissue (EAT) on the pathophysiology of AF might be different from that of other adipose tissues. The purpose of this study was to explore the distribution features of different adipose tissues in AF patients and their relationships with left atrial (LA) remodeling and function.MethodsA total of 205 participants (including 112 AF and 93 non-AF patients) were recruited. Color doppler ultrasound was used to measure the thickness of subcutaneous, extraperitoneal, and intra-abdominal adipose tissue. Cardiac CT scan was performed to measure the mean thickness of EAT surrounding the whole heart (total-EAT) and specific regions, including left atrium (LA-EAT), left ventricle, right ventricle, interventricular groove, and atrioventricular groove. LA anatomical remodeling and function were measured by echocardiography, while electrical remodeling was evaluated by P-wave duration and dispersion using Electrocardiography (obtained after cardioversion or ablation in AF patients). Relationship between the thickness of different adipose tissues and LA remodeling and function was analyzed.ResultsThe thickness of subcutaneous, extraperitoneal, and intra-abdominal adipose tissue was similar between AF and non-AF patients, and had no or only weak association with LA remodeling and dysfunction. However, compared to non-AF participants, total-EAT thickness significantly increased in both paroxysmal and persistent AF patients (non-AF vs. paroxysmal AF vs. persistent AF: 6.31 ± 0.63 mm vs. 6.76 ± 0.79 mm vs. 7.01 ± 1.18 mm, P &lt; 0.001), which was positively correlated with the LA size and P-wave duration and dispersion, and negatively correlated with LA ejection fraction and peak strain rate. More interestingly, EAT thickness in AF patients did not increase uniformly in different regions of the heart. Compared to EAT surrounding the other regions, LA-EAT was found to accumulate more greatly, and had a closer relationship to LA remodeling and dysfunction. Multivariate logistic regression analysis also showed that LA-EAT was significantly correlated with the presence of AF (OR = 4.781; 95% CI 2.589–8.831, P &lt; 0.001).ConclusionRather than other adipose tissues, accumulation and redistribution of EAT, especially surrounding the LA, is associated with LA remodeling and dysfunction in AF patients.
Enhanced understanding of changes in tropical cyclones’ landfall frequency over mainland China
Authors: Li Guangxin , Li Qinglan , Zhao Wei , Zhou Guanbo , Qian Qifeng , Qian Chuanhai , He Lunkai 
Published: August 2022

The climatological characteristics and interannual variations of tropical cyclones (TCs) making landfall in mainland China during the peak TC seasons (July–October) from 1980 to 2020 are examined using the China Meteorological Administration (CMA) best-track dataset. There were 270 TCs landfalling in mainland China during the study period, with 226 TCs landfalling in South China (SC) and 44 TCs landfalling in East China (EC). During 1980–2020, the number of TCs affecting mainland China gradually decreased, although the trend is not significant. The number of TCs impacting SC is experiencing a significant decrease, while the number of TC affecting EC is stable. Based on the change-point analysis, the TC landfall frequency in mainland China and SC incurred significant decreases in 1995/1996 and 1996/1997, respectively. The significant reduction in the number of landfalling TCs over SC and the insignificant reduction in the TC landfall frequency over mainland China are mainly due to the great reduction in the TC formation frequency over the western North Pacific (WNP). Meanwhile, the yearly mean of TCs’ landfalling latitudes is moving northward slightly, possibly linked to the slight poleward shift of their genesis locations. A large area of negative anomalies in the lower-tropospheric absolute vorticity and positive variations in the vertical wind shear (VWS) over the tropical WNP are possibly responsible for the reduction in TC genesis over the WNP. Moreover, the apparent opposite anomalies of the two variables over the region north of 20°N and south of 20°N might contribute to the slight poleward shift of genesis locations of landfalling TCs during the 41 years. The variations in the large-scale steering flow are favorable for more TCs moving northwestward and making landfall in EC than before. Meanwhile, the decrease in TC landfall frequency over mainland China is found to be significantly correlated to the pronounced warming over the tropical Indian Ocean.
Comprehensive evaluation of vegetation responses to meteorological drought from both linear and nonlinear perspectives
Authors: Zhou Zhaoqiang , Ding Yibo , Fu Qiang , Wang Can , Wang Yao , Cai Hejiang , Liu Suning , Shi Haiyun 
Published: August 2022

The frequent occurrence of drought events in recent years has caused significant changes in plant biodiversity. Understanding vegetation dynamics and their responses to climate change is of great significance to reveal the behaviour mechanism of terrestrial ecosystems. In this study, NDVI and SIF were used to evaluate the dynamic changes of vegetation in the Pearl River Basin (PRB). The relationship between vegetation and meteorological drought in the PRB was evaluated from both linear and nonlinear perspectives, and the difference of vegetation response to meteorological drought in different land types was revealed. Cross wavelet analysis was used to explore the teleconnection factors (e.g., large-scale climate patterns and solar activity) that may affect the relationship between meteorological drought and vegetation dynamics. The results show that 1) from 2001 to 2019, the vegetation cover and photosynthetic capacity of the PRB both showed increasing trends, with changing rates of 0.055/10a and 0.036/10a, respectively; 2) compared with NDVI, the relationship between SIF and meteorological drought was closer; 3) the vegetation response time (VRT) obtained based on NDVI was mainly 4–5 months, which was slightly longer than that based on SIF (mainly 3–4 months); 4) the VRT of woody vegetation (mainly 3–4 months) was longer than that of herbaceous vegetation (mainly 4–5 months); and 5) vegetation had significant positive correlations with the El Niño Southern Oscillation (ENSO) and sunspots but a significant negative correlation with the Pacific Decadal Oscillation (PDO). Compared with sunspots, the ENSO and the PDO were more closely related to the response relationship between meteorological drought and vegetation. The outcomes of this study can help reveal the relationship between vegetation dynamics and climate change under the background of global warming and provide a new perspective for studying the relationship between drought and vegetation.
Comparative efficacy and safety of glucose-lowering drugs in children and adolescents with type 2 diabetes: A systematic review and network meta-analysis
Authors: Wu Sijia , He Yina , Wu Yutong , Ji Yiman , Hou Lei , Liu Xinhui , Ge Yilei , Yu Yuanyuan , Yu Yifan , Wei Yun , Qian Fengtong , Luo Qingxin , Feng Yue , Feng Yiping , Wang Jiongjiong , Huo Meiling , Li Hongkai , Xue Fuzhong , Liu Yunxia 
Published: August 2022

ObjectiveType 2 diabetes is more common in adults, but is becoming the major concern in children and adolescent recently. This study aimed to provide additional pharmaceutical management for children and adolescents with type 2 diabetes by assessing the efficacy and safety of several glucose-lowering drugs.MethodsSearches were performed in PubMed, Medline, Ovid, Cochrane Controlled Register of Trials (CENTRAL), and ClinicalTrials.gov that reported the efficacy and safety of drugs for children and adolescents with type 2 diabetes. Pooled effects were calculated by frequentist fixed effects network meta-analyses and additive network meta-analyses.ResultsA total of 12 trials assessing eight glucose-lowering drugs were included, which compose of seven trials with monotherapy and five trials with combination therapies. Network meta-analysis results showed compared to placebo, saxagliptin+metformin (mean difference (MD) -1.91% [-2.85%, -0.97%]), liraglutide+metformin (MD -1.45% [-1.65%, -1.26%]), and liraglutide (MD -0.90% [-1.35%, -0.45%]) were the top 3 drugs that significantly reduced hemoglobin A1c (HbA1c). Sitagliptin+metformin, dapagliflozin, exenatide-2mcg, linagliptin-5mg, metformin, exenatide-5/10mcg, glimepiride, and sitagliptin also showed significant reduction in HbA1c. There were no significant differences between treatments in the incidence of adverse events, except that liraglutide+metformin had significant adverse effect such as abdominal pain. In addition, dapagliflozin, sitagliptin+metformin, and saxagliptin+metformin showed better efficacy compared with FDA-approved drugs.ConclusionsThe top 10 treatments of type 2 diabetes in children and adolescents aged 10–17 years were saxagliptin+metformin, liraglutide+metformin, liraglutide, dapagliflozin, exenatide–2 mcg, sitagliptin+metformin, linagliptin–5 mg, linagliptin–1 mg, metformin, and exenatide–5/10 mcg.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=284897, identifier CRD42021284897.
Roux-en-Y gastric bypass alters intestinal glucose transport in the obese Zucker rat
Authors: Meng Qinghe , Culnan Derek M. , Ahmed Tamer , Sun Mingjie , Cooney Robert N. 
Published: August 2022

IntroductionThe gastrointestinal tract plays a major role in regulating glucose homeostasis and gut endocrine function. The current study examines the effects of Roux-en-Y gastric bypass (RYGB) on intestinal GLP-1, glucose transporter expression and function in the obese Zucker rat (ZR).MethodsTwo groups of ZRs were studied: RYGB and sham surgery pair-fed (PF) fed rats. Body weight and food intake were measured daily. On post-operative day (POD) 21, an oral glucose test (OGT) was performed, basal and 30-minute plasma, portal venous glucose and glucagon-like peptide-1 (GLP-1) levels were measured. In separate ZRs, the biliopancreatic, Roux limb (Roux) and common channel (CC) intestinal segments were harvested on POD 21.ResultsBody weight was decreased in the RYGB group. Basal and 30-minute OGT plasma and portal glucose levels were decreased after RYGB. Basal plasma GLP-1 levels were similar, while a 4.5-fold increase in GLP-1 level was observed in 30-minute after RYGB (vs. PF). The increase in basal and 30-minute portal venous GLP-1 levels after RYGB were accompanied by increased mRNA expressions of proglucagon and PC 1/3, GPR119 protein in the Roux and CC segments. mRNA and protein levels of FFAR2/3 were increased in Roux segment. RYGB decreased brush border glucose transport, transporter proteins (SGLT1 and GLUT2) and mRNA levels of Tas1R1/Tas1R3 and α-gustducin in the Roux and CC segments.ConclusionsReductions in intestinal glucose transport and enhanced post-prandial GLP-1 release were associated with increases in GRP119 and FFAR2/3 after RYGB in the ZR model. Post-RYGB reductions in the regulation of intestinal glucose transport and L cell receptors regulating GLP-1 secretion represent potential mechanisms for improved glycemic control.