bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tertiary lymphoid structures critical for prognosis in endometrial cancer patients
Authors: Horeweg Nanda , Workel Hagma H. , Loiero Dominik , Church David N. , Vermij Lisa , Léon-Castillo Alicia , Krog Ricki T. , de Boer Stephanie M. , Nout Remi A. , Powell Melanie E. , Mileshkin Linda R. , MacKay Helen , Leary Alexandra , Singh Naveena , Jürgenliemk-Schulz Ina M. , Smit Vincent T. H. B. M. , Creutzberg Carien L. , Koelzer Viktor H. , Nijman Hans W. , Bosse Tjalling , de Bruyn Marco ,   TransPORTEC consortium, Horeweg Nanda , Church David N. , de Boer Stephanie M. , Nout Remi A. , Powell Melanie E. , Mileshkin Linda R. , MacKay Helen , Leary Alexandra , Singh Naveena , Creutzberg Carien L. , Nijman Hans W. , Bosse Tjalling , de Bruyn Marco 
Published: March 2022

AbstractB-cells play a key role in cancer suppression, particularly when aggregated in tertiary lymphoid structures (TLS). Here, we investigate the role of B-cells and TLS in endometrial cancer (EC). Single cell RNA-sequencing of B-cells shows presence of naïve B-cells, cycling/germinal center B-cells and antibody-secreting cells. Differential gene expression analysis shows association of TLS with L1CAM overexpression. Immunohistochemistry and co-immunofluorescence show L1CAM expression in mature TLS, independent of L1CAM expression in the tumor. Using L1CAM as a marker, 378 of the 411 molecularly classified ECs from the PORTEC-3 biobank are evaluated, TLS are found in 19%. L1CAM expressing TLS are most common in mismatch-repair deficient (29/127, 23%) and polymerase-epsilon mutant EC (24/47, 51%). Multivariable Cox regression analysis shows strong favorable prognostic impact of TLS, independent of clinicopathological and molecular factors. Our data suggests a pivotal role of TLS in outcome of EC patients, and establishes L1CAM as a simple biomarker.
Spinal cord injury impairs cardiac function due to impaired bulbospinal sympathetic control
Authors: Fossey Mary P. M. , Balthazaar Shane J. T. , Squair Jordan W. , Williams Alexandra M. , Poormasjedi-Meibod Malihe-Sadat , Nightingale Tom E. , Erskine Erin , Hayes Brian , Ahmadian Mehdi , Jackson Garett S. , Hunter Diana V. , Currie Katharine D. , Tsang Teresa S. M. , Walter Matthias , Little Jonathan P. , Ramer Matt S. , Krassioukov Andrei V. , West Christopher R. 
Published: March 2022

AbstractSpinal cord injury chronically alters cardiac structure and function and is associated with increased odds for cardiovascular disease. Here, we investigate the cardiac consequences of spinal cord injury on the acute-to-chronic continuum, and the contribution of altered bulbospinal sympathetic control to the decline in cardiac function following spinal cord injury. By combining experimental rat models of spinal cord injury with prospective clinical studies, we demonstrate that spinal cord injury causes a rapid and sustained reduction in left ventricular contractile function that precedes structural changes. In rodents, we experimentally demonstrate that this decline in left ventricular contractile function following spinal cord injury is underpinned by interrupted bulbospinal sympathetic control. In humans, we find that activation of the sympathetic circuitry below the level of spinal cord injury causes an immediate increase in systolic function. Our findings highlight the importance for early interventions to mitigate the cardiac functional decline following spinal cord injury.
Magnetically tightened form-stable phase change materials with modular assembly and geometric conformality features
Authors: Lu Yongyu , Yu Dehai , Dong Haoxuan , Lv Jinran , Wang Lichen , Zhou He , Li Zhen , Liu Jing , He Zhizhu 
Published: March 2022

AbstractPhase change materials have attracted significant attention due to their promising applications in many fields like solar energy and chip cooling. However, they suffer leakage during the phase transition process and have relatively low thermal conductivity. Here, through introducing hard magnetic particles, we synthesize a kind of magnetically tightened form-stable phase change materials. They achieve multifunctions such as leakage-proof, dynamic assembly, and morphological reconfiguration, presenting superior high thermal (increasing of 1400–1600%) and electrical (>104 S/m) conductivity, and prominent compressive strength, respectively. Furthermore, free-standing temperature control and high-performance thermal and electric conversion systems based on these materials are developed. This work suggests an efficient way toward exploiting a smart phase change material for thermal management of electronics and low-grade waste heat utilization.
CEP128 is involved in spermatogenesis in humans and mice
Authors: Zhang Xueguang , Wang Lingbo , Ma Yongyi , Wang Yan , Liu Hongqian , Liu Mohan , Qin Lang , Li Jinghong , Jiang Chuan , Zhang Xiaojian , Shan Xudong , Liu Yuliang , Li Jinsong , Li Yaqian , Zheng Rui , Sun Yongkang , Sun Jianfeng , Leng Xiangyou , Liang Yan , Zhang Feng , Jiang Xiaohui , Yang Yihong , Shen Ying 
Published: March 2022

AbstractCentrosomal proteins are necessary components of the centrosome, a conserved eukaryotic organelle essential to the reproductive process. However, few centrosomal proteins have been genetically linked to fertility. Herein we identify a homozygous missense variant of CEP128 (c.665 G > A [p.R222Q]) in two infertile males. Remarkably, male homozygous knock-in mice harboring the orthologous CEP128R222Q variant show anomalies in sperm morphology, count, and motility. Moreover, Cep128 knock-out mice manifest male infertility associated with disrupted sperm quality. We observe defective sperm flagella in both homozygous Cep128 KO and KI mice; the cilia development in other organs is normal—suggesting that CEP128 variants predominantly affected the ciliogenesis in the testes. Mechanistically, CEP128 is involved in male reproduction via regulating the expression of genes and/or the phosphorylation of TGF-β/BMP-signalling members during spermatogenesis. Altogether, our findings unveil a crucial role for CEP128 in male fertility and provide important insights into the functions of centrosomal proteins in reproductive biology.
Mechanically interlocked networks cross-linked by a molecular necklace
Authors: Zhang Zhaoming , Zhao Jun , Guo Zhewen , Zhang Hao , Pan Hui , Wu Qian , You Wei , Yu Wei , Yan Xuzhou 
Published: March 2022

AbstractMolecular necklaces have attracted much research attention due to their unique topological structures. Although numerous molecular necklaces with exquisite structures have been constructed, it remains a major challenge to exploit the functions and applications associated with their fascinating architectural and dynamic characteristics. Herein, we report a class of mechanically interlocked networks (MINs) cross-linked by a molecular necklace, in which multiple crown ethers are threaded on a hexagonal metallacyclic framework to furnish a cross-linker with delicate interlocked structures. The molecular necklace cross-linker possesses multiple peculiar advantages: multivalent interactions and rigid metallacycle framework guarantee robust features of MINs while the motion and dissociation of the interlocked structures bring in notable mechanical adaptivity. Moreover, the MINs could respond to the stimuli of K+and Br−, which lead to the dethreading of crown ether and even the complete decomposition of molecular necklace, respectively, showing abundant active properties. These findings demonstrate the untapped potential of molecular necklaces as cross-linkers and open the door to extend their advanced applications in intelligent supramolecular materials.
Precision drugging of the MAPK pathway in head and neck cancer
Authors: Ngan Hoi-Lam , Law Chun-Ho , Choi Yannie Chung Yan , Chan Jenny Yu-Sum , Lui Vivian Wai Yan 
Published: March 2022

AbstractThe mitogen-activating protein kinase (MAPK) pathway is central for cell proliferation, differentiation, and senescence. In human, germline defects of the pathway contribute to developmental and congenital head and neck disorders. Nearly 1/5 of head and neck squamous cell carcinoma (HNSCC) harbors MAPK pathway mutations, which are largely activating mutations. Yet, previous approaches targeting the MAPK pathway in HNSCC were futile. Most recent clinical evidences reveal remarkable, or even exceptional pharmacologic vulnerabilities of MAPK1-mutated, HRAS-mutated, KRAS-germline altered, as well as BRAF-mutated HNSCC patients with various targeted therapies, uncovering diverse opportunities for precision drugging this pathway at multiple “genetically condemned” nodes. Further, recent patient tumor omics unveil novel effects of MAPK aberrations on direct induction of CD8+ T cell recruitment into the HNSCC microenvironment, providing evidences for future investigation of precision immunotherapy for this large subset of patients. MAPK pathway-mutated HNSCC should warrant precision therapy assessments in vigorous manners.
Systematic literature review of schizophrenia clinical practice guidelines on acute and maintenance management with antipsychotics
Authors: Correll Christoph U. , Martin Amber , Patel Charmi , Benson Carmela , Goulding Rebecca , Kern-Sliwa Jennifer , Joshi Kruti , Schiller Emma , Kim Edward 
Published: March 2022

AbstractClinical practice guidelines (CPGs) translate evidence into recommendations to improve patient care and outcomes. To provide an overview of schizophrenia CPGs, we conducted a systematic literature review of English-language CPGs and synthesized current recommendations for the acute and maintenance management with antipsychotics. Searches for schizophrenia CPGs were conducted in MEDLINE/Embase from 1/1/2004–12/19/2019 and in guideline websites until 06/01/2020. Of 19 CPGs, 17 (89.5%) commented on first-episode schizophrenia (FES), with all recommending antipsychotic monotherapy, but without agreement on preferred antipsychotic. Of 18 CPGs commenting on maintenance therapy, 10 (55.6%) made no recommendations on the appropriate maximum duration of maintenance therapy, noting instead individualization of care. Eighteen (94.7%) CPGs commented on long-acting injectable antipsychotics (LAIs), mainly in cases of nonadherence (77.8%), maintenance care (72.2%), or patient preference (66.7%), with 5 (27.8%) CPGs recommending LAIs for FES. For treatment-resistant schizophrenia, 15/15 CPGs recommended clozapine. Only 7/19 (38.8%) CPGs included a treatment algorithm.
Deep rTMS of the insula and prefrontal cortex in smokers with schizophrenia: Proof-of-concept study
Authors: Moeller Scott J. , Gil Roberto , Weinstein Jodi J. , Baumvoll Topaz , Wengler Kenneth , Fallon Natalka , Van Snellenberg Jared X. , Abeykoon Sameera , Perlman Greg , Williams John , Manu Lucian , Slifstein Mark , Cassidy Clifford M. , Martinez Diana M. , Abi-Dargham Anissa 
Published: March 2022

AbstractPatients with schizophrenia have a high prevalence of cigarette smoking and respond poorly to conventional treatments, highlighting the need for new therapies. We conducted a mechanistic, proof-of-concept study using bilateral deep repetitive transcranial magnetic stimulation (dTMS) of insular and prefrontal cortices at high frequency, using the specialized H4 coil. Feasibility of dTMS was tested for disruption of tobacco self-administration, insula target engagement, and insula circuit modulation, all of which were a priori outcomes of interest. Twenty patients completed the study, consisting of weekday dTMS sessions (randomization to active dTMS or sham; double-blind; 10 patients per group), a laboratory tobacco self-administration paradigm (pre/post assessments), and multimodal imaging (three MRI total sessions). Results showed that participants assigned to active dTMS were slower to initiate smoking their first cigarette compared with sham, consistent with smoking disruption. The imaging analyses did not reveal significant Time × Group interactions, but effects were in the anticipated directions. In arterial spin labeling analyses testing for target engagement, an overall decrease in insula blood flow, measured during a post-treatment MRI versus baseline, was numerically more pronounced in the active dTMS group than sham. In fMRI analyses, resting-state connectivity between the insula and default mode network showed a numerically greater change from baseline in the active dTMS group than sham, consistent with a functional change to insula circuits. Exploratory analyses further suggested a therapeutic effect of dTMS on symptoms of psychosis. These initial observations pave the way for future confirmatory studies of dTMS in smoking patients with schizophrenia.
Machine Learning algorithm unveils glutamatergic alterations in the post-mortem schizophrenia brain
Authors: De Rosa Arianna , Fontana Andrea , Nuzzo Tommaso , Garofalo Martina , Di Maio Anna , Punzo Daniela , Copetti Massimiliano , Bertolino Alessandro , Errico Francesco , Rampino Antonio , de Bartolomeis Andrea , Usiello Alessandro 
Published: March 2022

AbstractSchizophrenia is a disorder of synaptic plasticity and aberrant connectivity in which a major dysfunction in glutamate synapse has been suggested. However, a multi-level approach tackling diverse clusters of interacting molecules of the glutamate signaling in schizophrenia is still lacking. We investigated in the post-mortem dorsolateral prefrontal cortex (DLPFC) and hippocampus of schizophrenia patients and non-psychiatric controls, the levels of neuroactive d- and l-amino acids (l-glutamate, d-serine, glycine, l-aspartate, d-aspartate) by HPLC. Moreover, by quantitative RT-PCR and western blotting we analyzed, respectively, the mRNA and protein levels of pre- and post-synaptic key molecules involved in the glutamatergic synapse functioning, including glutamate receptors (NMDA, AMPA, metabotropic), their interacting scaffolding proteins (PSD-95, Homer1b/c), plasma membrane and vesicular glutamate transporters (EAAT1, EAAT2, VGluT1, VGluT2), enzymes involved either in glutamate-dependent GABA neurotransmitter synthesis (GAD65 and 67), or in post-synaptic NMDA receptor-mediated signaling (CAMKIIα) and the pre-synaptic marker Synapsin-1. Univariable analyses revealed that none of the investigated molecules was differently represented in the post-mortem DLPFC and hippocampus of schizophrenia patients, compared with controls. Nonetheless, multivariable hypothesis-driven analyses revealed that the presence of schizophrenia was significantly affected by variations in neuroactive amino acid levels and glutamate-related synaptic elements. Furthermore, a Machine Learning hypothesis-free unveiled other discriminative clusters of molecules, one in the DLPFC and another in the hippocampus. Overall, while confirming a key role of glutamatergic synapse in the molecular pathophysiology of schizophrenia, we reported molecular signatures encompassing elements of the glutamate synapse able to discriminate patients with schizophrenia and normal individuals.
Flat-surface-assisted and self-regulated oxidation resistance of Cu(111)
Authors: Kim Su Jae , Kim Yong In , Lamichhane Bipin , Kim Young-Hoon , Lee Yousil , Cho Chae Ryong , Cheon Miyeon , Kim Jong Chan , Jeong Hu Young , Ha Taewoo , Kim Jungdae , Lee Young Hee , Kim Seong-Gon , Kim Young-Min , Jeong Se-Young 
Published: March 2022

AbstractOxidation can deteriorate the properties of copper that are critical for its use, particularly in the semiconductor industry and electro-optics applications1–7. This has prompted numerous studies exploring copper oxidation and possible passivation strategies8. In situ observations have, for example, shown that oxidation involves stepped surfaces: Cu2O growth occurs on flat surfaces as a result of Cu adatoms detaching from steps and diffusing across terraces9–11. But even though this mechanism explains why single-crystalline copper is more resistant to oxidation than polycrystalline copper, the fact that flat copper surfaces can be free of oxidation has not been explored further. Here we report the fabrication of copper thin films that are semi-permanently oxidation resistant because they consist of flat surfaces with only occasional mono-atomic steps. First-principles calculations confirm that mono-atomic step edges are as impervious to oxygen as flat surfaces and that surface adsorption of O atoms is suppressed once an oxygen face-centred cubic (fcc) surface site coverage of 50% has been reached. These combined effects explain the exceptional oxidation resistance of ultraflat Cu surfaces.
The Carbon Catalogue, carbon footprints of 866 commercial products from 8 industry sectors and 5 continents
Authors: Meinrenken Christoph J. , Chen Daniel , Esparza Ricardo A. , Iyer Venkat , Paridis Sally P. , Prasad Aruna , Whillas Erika 
Published: March 2022

AbstractProduct carbon footprints (PCFs) are playing an increasing role in decisions around sustainability for companies and consumers. Using data reported to CDP, we have previously built a dataset of 866 PCFs, from 145 companies, 30 industry groups, and 28 countries, showing trends of how upstream and downstream emissions vary by industry and how life cycle assessment (LCA) appears to aid companies in achieving steeper carbon reductions through improvements throughout a product’s value chain. Here, we present the greenhouse gas emissions and respective meta data for every product in this dataset. The Carbon Catalogue provides each product with name and description, PCF (in kg CO2e) and the respective LCA protocol/standard, product weight, as well as the name, industry, and country of incorporation of its manufacturer. For a subset of 421 products, the Carbon Catalogue further includes the PCF’s reported breakdown into two to nine separate stages of the product’s life cycle. For another subset of 250 products, the Carbon Catalogue includes how the respective PCFs changed and why the changes occurred.
A novel variant of the Calvin–Benson cycle bypassing fructose bisphosphate
Authors: Ohta Jun 
Published: March 2022

AbstractThe Calvin–Benson cycle (CB cycle) is quantitatively the most important metabolic pathway for CO2 fixation. In the canonical CB cycle, fructose 6-phosphate (F6P), fructose 1,6-bisphosphate (FBP), sedoheptulose 7-phosphate (S7P), and sedoheptulose 1,7-bisphosphate (SBP) appear as essential intermediates, where F6P is formed from FBP by the fructose 1,6-bisphosphatase (FBPase) reaction, and S7P is formed from SBP by the sedoheptulose 1,7-bisphosphatase (SBPase) reaction. Although the involvement of SBP and SBPase in the canonical CB cycle is consistent with the reported dependency of photosynthetic carbon metabolism on SBPase, the involvement of FBP and FBPase is not completely consistent with the reported FBP- or FBPase-related findings such as, although with a diminished growth rate, an Arabidopsis mutant lacking FBPase grew photoautotrophically in soil. Here, we show a novel variant of the CB cycle involving SBP, SBPase, and transaldolase, but neither FBP nor FBPase. This novel variant, named the S7P-removing transaldolase variant, bypasses FBP. This variant explains the FBP- or FBPase-related findings more easily than the canonical CB cycle as well as the dependency of photosynthetic carbon metabolism on SBPase and further suggests that co-overexpression of SBPase and transaldolase can be a strategy for enhancing photosynthetic carbon metabolism, which is important for the global environment.
Design and test of intelligent inspection and monitoring system for cotton bale storage based on RFID
Authors: Zhang Weipeng , Zhao Bo , Yang Qizhi , Zhou Liming , Jiang Hanlu , Niu Kang , Ding Jian 
Published: March 2022

AbstractTo solve the inspection problems in cotton storage, as well as the need for environmental monitoring in the process of modern cotton bale storage, an intelligent inspection and temperature and humidity intelligent monitoring system based on RFID cotton bale was developed by adopting RFID (Radio Frequency Identification) technology, wireless temperature and humidity real-time monitoring technology and handheld terminal intelligent inspection technology. The system was composed of RFID positioning inspection module and temperature and humidity real-time monitoring and transmission module. The artificial neural network (ANN) based on the particle swarm optimization (PSO) algorithm was used to process the monitoring data of the system by Gaussian filtering, and an accurate classification model of RSSI and label position was established. The test results showed that: Through the comparative analysis of the RFID indoor positioning algorithm, the positioning error of the PSO-ANN algorithm was small. In the actual cotton bale warehouse test, the relative error of positioning and monitoring for RFID cotton bale intelligent inspection and monitoring system was less than 6.7%, which effectively improved the working efficiency of inspection personnel and the security of cotton bale storage. The relative error of temperature and humidity was less than 8% and less than 7%, which could display the temperature and humidity information in real time and meet the real-time demand. This study improved the management personnel's effective positioning and inspection of the cotton bale, prevented the loss of cotton bale, reduced the deterioration probability of cotton bale, and effectively improved the storage management level of the cotton bale. It was of great practical significance to realize the networking, automation, and intelligence of cotton bale storage management.
Grasping learning, optimization, and knowledge transfer in the robotics field
Authors: Pozzi Luca , Gandolla Marta , Pura Filippo , Maccarini Marco , Pedrocchi Alessandra , Braghin Francesco , Piga Dario , Roveda Loris 
Published: March 2022

AbstractService robotics is a fast-developing sector, requiring embedded intelligence into robotic platforms to interact with the humans and the surrounding environment. One of the main challenges in the field is robust and versatile manipulation in everyday life activities. An appealing opportunity is to exploit compliant end-effectors to address the manipulation of deformable objects. However, the intrinsic compliance of such grippers results in increased difficulties in grasping control. Within the described context, this work addresses the problem of optimizing the grasping of deformable objects making use of a compliant, under-actuated, sensorless robotic hand. The main aim of the paper is, therefore, finding the best position and joint configuration for the mentioned robotic hand to grasp an unforeseen deformable object based on collected RGB image and partial point cloud. Due to the complex grasping dynamics, learning-from-simulations approaches (e.g., Reinforcement Learning) are not effective in the faced context. Thus, trial-and-error-based methodologies have to be exploited. In order to save resources, a samples-efficient approach has to be employed. Indeed, a Bayesian approach to address the optimization of the grasping strategy is proposed, enhancing it with transfer learning capabilities to exploit the acquired knowledge to grasp (partially) new objects. A PAL Robotics TIAGo (a mobile manipulator with a 7-degrees-of-freedom arm and an anthropomorphic underactuated compliant hand) has been used as a test platform, executing a pouring task while manipulating plastic (i.e., deformable) bottles. The sampling efficiency of the data-driven learning is shown, compared to an evenly spaced grid sampling of the input space. In addition, the generalization capability of the optimized model is tested (exploiting transfer learning) on a set of plastic bottles and other liquid containers, achieving a success rate of the 88%.