bluuebunny/crossref_metadata_2025_split huggingface dataset:
A tradeoff between acoustic and linguistic feature encoding in spoken language comprehension
Authors: Tezcan Filiz , Weissbart Hugo , Martin Andrea E. 
Published: August 2022

ABSTRACTWhen we comprehend language from speech, the phase of the neural response aligns with particular features of the speech input, resulting in a phenomenon referred to as neural tracking. In recent years, a large body of work has demonstrated the tracking of the acoustic envelope and abstract linguistic units at the phoneme and word levels, and beyond. However, the degree to which speech tracking is driven by acoustic edges of the signal, or by internally-generated linguistic units, or by the interplay of both, remains contentious. In this study, we used naturalistic story-listening to investigate (1) whether phoneme-level features are tracked over and above acoustic edges, (2) whether word entropy, which can reflect sentence- and discourse-level constraints, impacted the encoding of acoustic and phoneme-level features, and (3) whether the tracking of acoustic edges was enhanced or suppressed during comprehension of a first language (Dutch) compared to a statistically-familiar but uncomprehended language (French). We first show that encoding models with phoneme-level linguistic features, in addition to acoustic features, uncovered an increased neural tracking response; this signal was further amplified in a comprehended language, putatively reflecting the transformation of acoustic features into internally-generated phoneme-level representations. Phonemes were tracked more strongly in a comprehended language, suggesting that language comprehension functions as a neural filter over acoustic edges of the speech signal as it transforms sensory signals into abstract linguistic units. We then show that word entropy enhances neural tracking of both acoustic and phonemic features when sentence- and discourse-context are less constraining. When language was not comprehended, acoustic features, but not phonemic ones, were more strongly modulated, but in contrast, when a first language is comprehended, phoneme features are more strongly modulated. Taken together, our findings highlight the flexible modulation of acoustic, and phonemic features by sentence and discourse-level constraint in language comprehension, and document the neural transformation from speech perception to language comprehension, consistent with an account of language processing as a neural filter from sensory to abstract representations.
Human fetal cerebellar cell atlas informs medulloblastoma origin and oncogenesis
Authors: Luo Zaili , Xia Mingyang , Shi Wei , Zhao Chuntao , Wang Jiajia , Xin Dazhuan , Dong Xinran , Xiong Yu , Zhang Feng , Berry Kalen , Ogurek Sean , Liu Xuezhao , Rao Rohit , Xing Rui , Wu Lai Man Natalie , Cui Siying , Xu Lingli , Lin Yifeng , Ma Wenkun , Tian Shuaiwei , Xie Qi , Zhang Li , Xin Mei , Wang Xiaotao , Yue Feng , Zheng Haizi , Liu Yaping , Stevenson Charles B. , de Blank Peter , Perentesis John P. , Gilbertson Richard J. , Ma Jie , Li Hao , Zhou Wenhao , Taylor Michael D. , Lu Q. Richard 
Published: August 2022

AbstractMedulloblastoma (MB) is the most common malignant childhood brain tumor1,2, yet the origin of the most aggressive subgroup-3 form remains elusive, impeding development of effective targeted treatments. Previous analyses of mouse cerebella3,4or human counterparts from frozen tissue nuclei5have not fully defined the compositional heterogeneity of MBs. Here, we undertook an unprecedented single-cell profiling of freshly-isolated human fetal cerebella to establish a reference map delineating hierarchical cellular states in MBs. We identified a unique transitional cerebellar progenitor connecting neural stem cells to neuronal lineages in developing fetal cerebella. Intersectional analysis revealed that the transitional progenitors were enriched in aggressive MB subgroups, including group-3 and metastatic tumors. Single-cell multi-omics revealed underlying regulatory networks in the transitional progenitor populations, including transcriptional determinants HNRNPH1 and SOX11, which are correlated with clinical prognosis in group-3 MBs. Genomic and Hi-C profiling identifiedde novolong-range chromatin-loops juxtaposing HNRNPH1/SOX11-targeted super-enhancers tocis-regulatory elements of MYC, an oncogenic driver for group-3 MBs. Targeting the transitional progenitor regulators inhibited MYC expression and MYC-driven group-3 MB growth. Our integrated single-cell atlases of human fetal cerebella and MBs reveal potential cell populations predisposed to transformation and regulatory circuitries underlying tumor cell states and oncogenesis, highlighting hitherto unrecognized transitional progenitor intermediates predictive of disease prognosis and potential therapeutic vulnerabilities.
The C-type lectin domain of CD62P (P-selectin) is an integrin ligand
Authors: Takada Yoko K , Takada Yoshikazu 
Published: August 2022

AbstractCD62P (P-selectin) is confined to the inside of platelets and endothelial cells, and is translocated to the surface upon activation of platelets or endothelial cells. In current models, CD62P recognizes sialyl-Lewis X on PSGL-1 and mediates rapid rolling of leukocyte over vascular surfaces during the initial steps in inflammation. Docking simulation using integrin αvβ3 as a target predicted that the C-type lectin domain of CD62P is a potential integrin ligand. It has not been tested if CD62P binds to integrins. Here we describe that the lectin domain of CD62P specifically bound to soluble integrins αvβ3, αIIbβ3, α4β1 and α5β1. Known inhibitors of CD62P-PSGL-1 interaction did not suppress the binding of the lectin domain to integrins. We found that the R16E/K17E mutation in the predicted integrin-binding interface of the lectin domain strongly inhibited CD62P binding to αIIbβ3 and αvβ3 in 1 mM Mn2+. R16E/K17E is outside of the glycan binding site. Mutating Glu-88 to Asp (the E88D mutation) in the lectin domain, which is known to strongly disrupt glycan binding, only slightly affected integrin binding, indicating that glycan binding and integrin binding sites are distinct. Also, the lectin domain of CD62P supported cell adhesion in a cation-dependent manner. CD62P-integrin interaction is potentially important since integrins are widely expressed compared to PSGL-1, which is limited to leukocytes. These findings indicate that CD62P-integrin interaction plays potentially important role in a wide variety of cell-cell interaction in addition to CD62P-glycan interaction.
Differential protein expression of GABA A receptor alpha 1 subunit in rat spermatozoa associated with proteomic analysis in testis following methamphetamine administration
Authors: Kaewman Paweena , Nudmamud-Thanoi Sutisa , Thongleart Jitnapar , Charoenlappanit Sawanya , Roytrakul Sittiruk , Thanoi Samur 
Published: August 2022

AbstractMethamphetamine (METH) can induce spermatogenesis impairment, testicular apoptosis, and abnormal sperm quality. It also promotes changes in the expression of receptors for sex hormones and neurotransmitters, including GABA receptors in the testis. Proteomic assessment focusing on proteins involved in the calcium signalling pathway in the testis can facilitate diagnostic factors contributing to testicular and sperm functions, especially those related to spermatogenesis and fertilisation. In this study, we proposed to determine the localization and differential expression of GABA A receptor alpha 1 subunit (GABA A-α1) in the spermatozoa of METH-administrated rats. The differential proteomic profile of the testis was also observed by focusing on proteins in the KEGG pathways belonging to the calcium signalling pathway. There were 212 differentially expressed proteins in the rat testis, based on the cut-off value of 1.2-fold change. Most of those proteins, 13 proteins, were classified in the calcium signalling pathway, including 4 down-regulated and 9 up-regulated proteins. An immunolocalization study of the GABA A-α1 receptor revealed its localization in the equatorial segment of the acrosome in the rat spermatozoa, a region involved in acrosome reaction. An increase in GABA A-α1 receptor in rat spermatozoa was correlated with an increase in abnormal sperm motility and morphology after methamphetamine exposure. All our findings demonstrate that METH influences intracellular calcium homeostasis by acting through the calcium signalling pathway-associated proteins. Moreover, it might disrupt ion homeostasis in sperm through the GABA A-α1 receptor, triggering a change in intracellular calcium and chloride ions. These changes may cause abnormalities in spermatogenesis, testicular apoptosis, and sperm quality impairment.
Cryo-EM complex structure of active GPR75 with a nanobody
Authors: Lv Zilin , He Yuntong , Xiang Yuning , Li Jing , Zhang Shuhao , Meng Fanhao , Lan Baoliang , Guo Hanbo , He Dong , Wang Yanxia , Zhao Huimin , Zhuo Wei , Liu Yujie , Liu Xiangyu , Ni Xiaodan , Heng Jie 
Published: August 2022

AbstractAlthough there has been enormous progress in the last half-century in the drug discovery targeting obesity and associated co-morbidities, the clinical treatment of obesity remains tremendously challenging. GPR75 is an orphan receptor and is suggested to be a potential novel target for the control of obesity and related metabolic disorders. Inhibition of the GPR75 signaling pathway by small molecules, antibodies, or genetic manipulations may provide a therapeutic strategy for obesity. Here, we report the active-like Cryo-EM structure of human GPR75 with an intracellular nanobody, which reveals the receptor activation mechanism. The extensive interaction network required to achieve the active structure helps explain the allosteric coupling between the orthosteric pocket and the G-protein coupling domain. The well-defined orthosteric ligand binding pocket of human GPR75 provides a structural basis for anti-obesity drug discovery.
Modeling the development of cortical responses in primate dorsal (“where”) pathway to optic flow using hierarchical neural field models
Authors: Gundavarapu Anila , Chakravarthy V Srinivasa 
Published: August 2022

ABSTRACTAlthough there is a plethora of modelling literature dedicated to the object recognition processes of the ventral (“what”) pathway of primate visual systems, modelling studies on the motion sensitive regions like the Medial superior temporal area (MST) of the dorsal (“where”) pathway are relatively scarce. Neurons in the MST area of the macaque monkey respond selectively to different types of optic flow sequences such as radial and rotational flows. We present three models that are designed to simulate the computation of optic flow performed by the MST neurons in primates. The first two models are each composed of 3 stages: the first stage comprises the Direction Selective Mosaic Network (DSMN), the second stage comprises the Cell Plane Network (CPNW) or the Hebbian Network (HBNW) and the third stage comprises the optic flow network (OF). The three stages roughly correspond to V1-MT-MST areas respectively in the primate motion pathway. Both these models are trained stage by stage using a biologically plausible variation of Hebbian learning. On the other hand, model-3 consists of the Velocity Selective Mosaic Network (VSMN) followed by a convolutional neural network (CNN) which is trained using supervised backpropagation algorithm. We created various dot configurations that can move in translational, radial, and rotational trajectories to make training and test set. The simulation results show that, while neurons in model-1 and model-2 could account for MSTd cell properties found neurobiologically, model-3 neuron responses are consistent with the idea of functional hierarchy in the macaque motion pathway. These results also suggest that the deep learning models could offer a computationally elegant and biologically plausible solution to simulate the development of cortical responses of the primate motion pathway.
A new haplotype-resolved turkey genome to enable turkey genetics and genomics research
Authors: Barros Carolina P. , Derks Martijn F.L. , Mohr Jeff , Wood Benjamin , Crooijmans Richard P.M.A. , Megens Hendrik-Jan , Bink Marco C.A.M. , Groenen Martien A.M. 
Published: August 2022

BackgroundThe domesticated turkey (Meleagris gallopavo) is a species of significant agricultural importance and is the second largest contributor, behind broiler chickens, to world poultry meat production. The previous genome is of draft quality and partly based on the chicken (Gallus gallus) genome. A high-quality reference genome of Meleagris gallopavo is essential for turkey genomics and genetics research and the breeding industry.ResultsBy adopting the trio-binning approach, we were able to assemble a high-quality chromosome-level F1 assembly and two parental haplotype assemblies, leveraging long-read technologies and genomewide chromatin interaction data (Hi-C). These assemblies cover 35 chromosomes in a single scaffold and show improved genome completeness and continuity. The three assemblies are of higher quality than the previous draft quality assembly and comparable to the current chicken assemblies (GRCg6a and GRCg7). Comparative analyses reveal a large inversion of around 19 Mbp on the Z chromosome not found in other Galliformes. Structural variation between the parent haplotypes were identified in genes involved in growth providing new target genes for breeding.ConclusionsCollectively, we present a new high quality chromosome level turkey genome, which will significantly contribute to turkey and avian genomics research and benefit the turkey breeding industry.
The pharmacokinetics and pharmacodynamics of 4-methylumbelliferone and its glucuronide metabolite in mice
Authors: Nagy Nadine , Kaber Gernot , Haddock Naomi L. , Hargil Aviv , Rajadas Jayakumar , Malhotra Sanjay V. , Unger Marc A. , Frymoyer Adam R. , Bollyky Paul L. 
Published: August 2022

AbstractHyaluronan (HA) is an extracellular matrix glycosaminoglycan, with important roles in chronic inflammation, cancer and autoimmunity. 4-methylumbelliferone (4-MU), a small molecule inhibitor of HA synthases, is widely used to study HAs interactions with the surrounding tissues and the immune cells. There is substantial experimental and therapeutic interest in using oral 4-MU to inhibit HA synthesis, but pharmacokinetic and pharmacodynamic data on treatment routes have been lacking. Moreover, it recently became clear that the main metabolite of 4-MU, 4-methlyumbelliferyl glucuronide (4-MUG), is bioactive. We therefore sought to define the pharmacokinetics and pharmacodynamics of 4-MU and its active metabolite 4-MUG in mice. Single dose mouse studies showed that 4-MU administered intravenously (i.v.) resulted in 100-fold higher 4-MU exposure compared to oral (p.o.) administration. The 4-MU ratio AUC i.v./AUC p.o. was 96/1. 4-MUG exposures were much higher than 4-MU exposures after both 4-MU i.v. and p.o. administration, but only small differences in 4-MUG exposure were seen after 4-MU i.v. versus p.o. administration. The 4-MUG metabolite was also administered as a single dose both i.v. and p.o. and showed a 25.9% bioavailability. Compared to 4-MUG p.o. dosing, 1.14 higher 4-MUG exposures were seen after 4-MU p.o. dosing. 4-MU exposure after 4-MUG p.o. administration was minimal but similar to 4-MU exposure after 4-MU p.o. administration. In mice treated for several weeks with 4-MU in chow, the 4-MU concentration immediately drops after treatment was stopped, whereas the 4-MUG concentration showed a peak 1 hour after treatment stop. In a build-up study, 4-MU and 4-MUG treatment in mice lead to a plateau of 4-MU concentration starting at 4 days post treatment start. These 4-MU and 4-MUG concentration findings in vivo will inform future clinical studies and experimental work with 4-MU.
Examining craniofacial variation among crispant and mutant zebrafish models of human skeletal diseases
Authors: Diamond Kelly M. , Burtner Abigail E. , Siddiqui Daanya , Alvarado Kurtis , Leake Sanford L. , Rolfe Sara , Zhang Chi , Kwon Ronald Y. , Maga A. Murat 
Published: August 2022

AbstractGenetic diseases affecting the skeletal system present with a wide range of symptoms that make diagnosis and treatment difficult. Genome-wide association and sequencing studies have identified genes linked to human skeletal diseases. Gene editing of zebrafish models allows researchers to further examine the link between genotype and phenotype, with the long-term goal of improving diagnosis and treatment. While current automated tools enable rapid and in-depth phenotyping of the axial skeleton, characterizing the effects of mutations on the craniofacial skeleton has been more challenging. The objective of this study was to evaluate a semi-automated screening tool can be used to quantify craniofacial variations in zebrafish models using four genes that have been associated with human skeletal diseases (meox1, plod2, sost, andwnt16) as test cases. We used traditional landmarks to ground truth our dataset and pseudolandmarks to quantify variation across the 3D cranial skeleton between the groups (somatic crispant, germline mutant, and control fish). The proposed pipeline identified variation between the crispant or mutant fish and control fish for four genes. Variation in phenotypes parallel human craniofacial symptoms for two of the four genes tested. This study demonstrates the potential as well as the limitations of our pipeline as a screening tool to examine multi-dimensional phenotypes associated with the zebrafish craniofacial skeleton.
Predicting turnover intention of indonesian millennials workforce in the manufacturing industry: a PLS-SEM approach
Authors: Ardi Romadhani , Anggraini Niken 
Published: August 2022

Purpose
This study aims to assess the factors that can affect the turnover intention of millennial talents working in the Indonesian manufacturing industry.

Design/methodology/approach
This work analyzes 115 responses from millennial talents working in the Indonesian manufacturing industry on the issue of turnover intention. Data were collected through an online questionnaire. The collected data were then tested using partial least square-structural equation modeling.

Findings
The findings suggest that competency development, work–life balance and meaningful work have a direct impact on the employee satisfaction of millennials in the Indonesian manufacturing industry. However, compensation and benefits have no significant effect on job satisfaction.

Research limitations/implications
This study has limitations in data collection, concentrated merely in the Jakarta Greater Area. Hence, it would potentially influence the generalization of the results.

Originality/value
This study reveals the potential lack of awareness within Indonesian manufacturing companies in providing a valuable compensation and benefits program for millennials.
Antecedents of customer mortgage shopping satisfaction: the mediating role of search intensity, evidence from the NSMO survey
Authors: Reddy N Srinivasa , Thanigan Jayanthi 
Published: August 2022

Purpose
The purpose of this paper is to examine the antecedents of customer satisfaction during mortgage purchases. Mortgage demand in the USA has reached an all-time high because of an increase in housing demand after COVID-19. Nonetheless, several customers are dissatisfied with their service providers. Customers who actively search the market gain more information about mortgage providers and use this information to define expectations for lenders. The only way there will be customer satisfaction is if lenders meet these expectations. Therefore, it is economically significant for mortgage lenders to discover the antecedents of mortgage satisfaction.

Design/methodology/approach
In this study, the partial least squares approach was used to test the hypothesis that satisfaction was influenced by objective knowledge, familiarity and search intensity among a sample of customers (n = 4,512) from the National Survey of Mortgage Originations who had purchased a mortgage in the USA between 2019 and 2020.

Findings
The results of structural modelling showed that familiarity (β = 0.23 and p = 0.01) with and knowledge (β = 0.16 and p = 0.01) of mortgages significantly affected consumer satisfaction during mortgage purchase. Search intensity (p = 0.01) mediated the relationship between knowledge, familiarity and satisfaction.

Research limitations/implications
The primary implication is that mortgage service providers should prioritise educating customers about the mortgage buying process on their websites and in person. So managers must actively assist clients in having realistic expectations. Second, mortgage companies should establish a presence on third-party mortgage comparison websites to ensure that customers actively consider alternatives, thereby increasing customer satisfaction.

Originality/value
This study is unique in being an exploratory study to examine the antecedents of mortgage satisfaction using a public data set. This study uniquely examines the National Survey of Mortgage Originations data set with partial least squares approach to examine underlying customer attitudes.
Making agency theory work for supply chain relationships: a systematic review across four disciplines
Authors: Matinheikki Juri , Kauppi Katri , Brandon–Jones Alistair , van Raaij Erik M. 
Published: August 2022

PurposeContemporary supply chain relationships inherently rely on delegation of work between organizations and, thus, are subject to agency problems for which a wide range of governance mechanisms exist. This review of agency theory (AT), across four distinct fields, explains the connection between governance mechanisms and supply chain relationship types.Design/methodology/approachThe study uses a systematic literature review (SLR) of articles using AT in a supply chain context from the operations and supply chain management, general management, marketing, and economics fields.FindingsThe authors categorize the governance mechanisms identified to create a typology of agency relationships in supply chains.Research limitations/implicationsThe developed typology provides parsimonious theory on different forms of supply chain agency relationships and takes a step towards a “supply chain-oriented agency theory” explaining and predicting relationship types and governance in supply chains. Furthermore, a future research agenda calls for more accurate measuring of agency costs, to examine residual gains alongside residual losses, to take a dual-sided perspective of agency relations and to adopt AT to examine more complex supply networks.Practical implicationsThe review provides a menu of governance mechanisms and describes situations under which these mechanisms could be deployed to guide managers when developing their supply chain relationships.Originality/valueThe first review to combine and elaborate views from four major disciplines using AT as a lens to supply chain relationships. Expanding the traditional set of governance mechanisms provides academics and practitioners with a bigger “menu” of options to consider.