bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-30c-5p targets SIRT1 to promote apoptosis in ovarian granulosa cells
Authors: Zhou Lifei , Zheng Bo , Luo Yan , Zhang Pingping , Dai Fangfang , Zhang mingming , Li Yali 
Published: January 2025

Abstract

Background: Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder characterized by abnormal follicular development and is the leading cause of infertility among women of reproductive age. miR-30c-5p, a newly discovered microRNA (miRNA), plays a crucial role in numerous pathological conditions, such as malignancies, reproductive dysfunctions, and metabolic irregularities. However, the role of miR-30c-5p in PCOS and the apoptosis of granulosa cells (GCs) is not fully understood.
Research methods: GCs were isolated from the follicular fluid of 23 patients with PCOS and 21 normal controls. The expression of miR-30c-5p and Sirtuin 1 (SIRT1) was detected using quantitative real-time polymerase chain reaction (qRT-PCR). A Spearman correlation analysis was conducted to evaluate the relationship between the expression levels of these markers. Proliferation and apoptosis in human granulosa tumor cell lines (KGN) were analyzed following the overexpression or underexpression of miR-30c-5p, utilizing the Cell Counting Kit-8, flow cytometry, and western blot techniques. TargetScan was used to identify potential targets of miR-30c-5p, which were subsequently validated through qRT-PCR, western blot, and a dual luciferase reporter gene assay.
Results: The results of our study suggest that the levels of miR-30c-5p aresignificantly elevated in individuals with PCOS compared with the control group. Conversely, we observed a significant decrease in the expression of SIRT1 in patients with PCOS compared with the control group. Moreover, miR-30c-5p expression was negatively correlated with SIRT1. The upregulation of miR-30c-5p suppressed the expression of SIRT1 and Bcl-2, inhibited GC proliferation, and increased the expression of Bax.
Conclusion: Our findings suggest that miR-30c-5p inhibits the proliferation of KGN cells by targeting SIRT1. Thus, this study enhances the current understanding of the mechanisms underlying apoptosis in PCOS and GCs.
Exploratory Study on Motivational Factors in Recreational Sports: A Gender and Region-based Analysis of Students at Vietnam National University, Ho Chi Minh City
Authors: Ha Minh Diu , Dao Chanh Thuc 
Published: January 2025

Introduction
The importance of recreational sports for overall human development is widely acknowledged. These activities provide numerous physical, mental, and social benefits, particularly for students. Problem Statement: While the general benefits of recreational sports are recognized, less is understood about the specific motivational factors driving student participation, particularly concerning the influence of gender and regional background. This study addresses this gap by investigating how these factors shape students' motivation to engage in recreational sports.

Methods
This study focuses on understanding the motivational factors influencing recreational sports participation among students at Vietnam National University - Ho Chi Minh City (VNU-HCMC), specifically examining the role of gender and regional background. Material and Methods: The research involved a sample of 918 students (455 males and 463 females) aged 18-22 from VNU-HCMC. Motivation was measured using the standardized ATPA-D questionnaire.

Results
The findings revealed that health, enjoyment, and social interaction are the three primary motivators for students' participation in recreational sports. However, significant differences were observed among subgroups. Male students prioritized social interaction, while female students placed higher importance on health benefits. Interestingly, aesthetics was a more significant motivator for female students from the North compared to those from the South and Central regions. The competitive aspect was more appealing to male students from the South. This trend was mirrored among female students, with those from the South and Central regions showing greater interest in competition than those from the North. Discussion: Despite the observed gender and region-based differences, the study also highlights similarities in recreational sports participation motives among young people across Vietnam. This suggests that regional background is not the sole determinant of recreational sports choices among VNU-HCMC students.

Conclusion
The study provides valuable insights into the diverse motivational factors influencing recreational sports participation among students. Understanding these factors, particularly the nuances related to gender and regional background is crucial for promoting and developing inclusive and engaging recreational sports programs.
Assessment of Reliability and Validity of Celiac Disease–Related YouTube Videos: Content Analysis
Authors: Polat Yunus Halil , Cankurtaran Rasim Eren 
Published: January 2025

Background
YouTube is an increasingly used platform for medical information. However, the reliability and validity of health-related information on celiac disease (CD) on YouTube have not been determined.

Objective
This study aimed to analyze the reliability and validity of CD-related YouTube videos.

Methods
On November 15, 2023, a search was performed on YouTube using the keyword “celiac disease.” This search resulted in a selection of videos, which were then reviewed by 2 separate evaluators for content, origin, and specific features. The evaluators assessed the reliability and quality of these videos using a modified DISCERN (mDISCERN) score, the Journal of the American Medical Association (JAMA) benchmark criteria score, the usefulness score, video power index (VPI), and the Global Quality Scale (GQS) score.

Results
In the analysis of 120 initially screened CD videos, 85 met the criteria for inclusion in the study after certain videos were excluded based on predefined criteria. While the duration of the videos uploaded by health care professionals was significantly longer than the other group (P=.009), it was concluded that the median scores for mDISCERN (4, IQR 4-5 vs 2, IQR 2-3; P<.001), GQS (4, IQR 4-5 vs 3, IQR 2-3; P<.001), JAMA (4, IQR 3-4 vs 2, IQR 2-3; P<.001), and usefulness (8, IQR 7-9 vs 6, IQR 3-6; P<.001) of the videos from this group were significantly higher than those from non–health care professionals. Video interaction parameters, including the median number of views, views per day, likes, dislikes, comments, and VPI, demonstrated no significant difference between the 2 groups.

Conclusions
This study showed that YouTube videos about CD vary significantly in reliability and quality depending on their source. Increasing the production of reliable videos by health care professionals may help to improve patient education and make YouTube a more reliable resource.
Unpacking Performance Factors of Innovation Systems and Studying Germany’s Attempt to Foster the Role of the Patient Through a Market Access Pathway for Digital Health Applications (DiGAs): Exploratory Mixed Methods Study
Authors: Gehder Sara , Goeldner Moritz 
Published: January 2025

Background
Health care innovation faces significant challenges, including system inertia and diverse stakeholders, making regulated market access pathways essential for facilitating the adoption of new technologies. The German Digital Healthcare Act, introduced in 2019, offers a model by enabling digital health applications (DiGAs) to be reimbursed by statutory health insurance, improving market access and patient empowerment. However, the factors influencing the success of these pathways in driving innovation remain unclear.

Objective
This study aims to identify the key performance factors of the innovation system shaped by the patient-relevant structural and procedural improvement (pSVV) pathway within the DiGA model. By examining how this pathway supports the entry of innovative digital health technologies, we seek to uncover the systemic dynamics that influence its effectiveness in fostering patient-centered digital health solutions.

Methods
This study, conducted from May 2023 to November 2024, used a mixed methods approach. A descriptive analysis assessed how DiGA manufacturers use positive health care effects, giving a market overview of the pSVV technology. A qualitative analysis using grounded theory and Gioia methodology provided insights into stakeholder perspectives, focusing on manufacturers and regulatory bodies. A functional-structural analysis examined how components of the innovation system, such as actors, institutions, interactions, and infrastructure, interact and impact the effectiveness of the pathway.

Results
The descriptive analysis showed that only 11 (20%) of the 56 DiGAs available in Germany used the pSVV pathway, with only 1 (2%) provisionally listed DiGA using pSVV as a primary end point; 6 of 9 (67%) pSVV key areas were used. The qualitative analysis revealed that manufacturers prioritize demonstrating medical benefits over pSVV due to evidence requirements and uncertainties around pSVV acceptance. Operational barriers hindered the adoption of pSVV, despite a positive reception among stakeholders. The systemic analysis identified key issues, including a lack of entrepreneurial focus on pSVV, limited regulatory experience, inadequate measurement methods, and entrenched practices prioritizing medical benefits, that hinder market formation and legitimacy.

Conclusions
This study identifies key factors for effectively implementing innovation systems through regulated market access pathways, including content and format security, clearer framework specification, active innovation process management, and market formation stimulation. Addressing these factors can reduce uncertainties and promote wider adoption of digital health technologies. The findings highlight the need for future research on patient empowerment and the development of methodologies beyond traditional therapeutic outcomes.
Antioxidant Activity of Premna serratifolia Linn. Leaf Extracts: A Comprehensive Analysis Using Various Testing Methods
Authors: Muhaimin Muhaimin , Iskandar Yoppi , Hazrina Aghnia , Hirzan Rifanza , Khatib Alfi , Syahri Wilda 
Published: January 2025

Premna serratifolia Linn leaf possesses medicinal properties, useful in the treatment of cardiovascular diseases, skin diseases, inflammatory diseases, arthritis, rheumatism, anorexia, jaundice, intestinal worms, bad breath, lung infections, febrifuge, hypolipidemic, antioxidants, antidiabetic, CNS depressant and thalasemia treatment, among other things. The purpose of this study was to investigate the antioxidant effect of P. serratifolia Linn leaf from Indonesia. The leaves of P. serratifolia Linn were extracted with 95% ethanol and fractionated with n-hexane and ethyl acetate. The antioxidant activity was evaluated by various antioxidant assays, including 1-diphenyl-2-picrylhydrazyl (DPPH), 2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), nitric oxide scavenging and reducing power ability method. The extracts and fractions contained phytoconstituents such as alkaloid, flavonoid, phenolic, tanin, saponin, and steroid compounds, according to phytochemical analysis. The antioxidant activities were compared to standard antioxidant ascorbic acid. P. serratifolia Linn ethanol leaves extract showed a significant antioxidant activity in DPPH, ABTS, reducing power and nitric oxide scavenging methods compared others. The findings of the present study suggest that P. serratifolia Linn could be a potential source of natural antioxidant that could have greater importance as therapeutic agent in preventing or slowing oxidative stress related degenerative diseases.
Conceptual Metaphor of “Quality” in Charles & Keith’s Instagram Caption: A Cognitive Semantic Analysis
Authors: Silalahi Elisa Debora 
Published: January 2025

This research presents a cognitive semantics study in Charles Keith's Instagram caption. It is conducted to observe the conceptual metaphors of “quality” in Charles Keith's Instagram caption, including the types and image schema of each conceptual metaphor. The data source is Charles Keith's Instagram caption uploaded in January 2023 – June 2023. The research used a qualitative method with a cognitive semantics approach. Based on the analysis, there are 10 conceptual metaphors of “quality” classified into three types of conceptual metaphors: 5 structural metaphors, 2 orientational metaphors, and 3 ontological metaphors. Moreover, 3 metaphors belong to the image schema of identity, 3 metaphors belong to the image schema of force, 2 metaphors belong to the image schema of space, and 2 metaphors belong to the image schema of existence. Those conceptual metaphors are utilized to highlight the quality of Charles Keith products by portraying the products’ excellence.
FIQH MUAMALAH ANALYSIS OF DEFERRED FERTILIZER TRANSACTIONS IN BALUKANG DONGGALA
Authors: Sahrin Moh , Aisya Sitti , Kristiane Desy 
Published: January 2025

This study examines the practice of borrowing and lending fertilizer and rice seeds with an agreement to pay after the harvest in Balukang Village, Sojol District, Donggala Regency, as well as its perspective in Islamic commercial law (fiqh muamalah). The aim of this research is to identify this practice within the community and analyze it from the perspective of fiqh muamalah. The method used is a juridical-sociological approach, with data collection techniques including observation, documentation, and in-depth interviews. The findings show that this practice has become a tradition in Balukang Village, where fertilizer and seeds are provided at the beginning of the contract, with payment made after the harvest in the form of rice. The agreement is made orally and recorded by the creditor without a written contract. This practice is more accurately described as a sale agreement with deferred payment, although the community commonly refers to it as a borrowing and lending arrangement. From the perspective of fiqh muamalah, this practice is allowed because it has become a customary practice that does not harm either party and has been mutually agreed upon by both parties.
Abstrak
Penelitian ini membahas praktik utang piutang pupuk dan benih padi dengan perjanjian pembayaran setelah panen di Desa Balukang, Kecamatan Sojol, Kabupaten Donggala, serta perspektif fikih muamalah terhadap praktik tersebut. Tujuan penelitian ini adalah untuk mengidentifikasi praktik tersebut di masyarakat dan menganalisisnya dari sudut pandang fikih muamalah. Metode yang digunakan adalah pendekatan yuridis sosiologis, dengan teknik pengumpulan data berupa observasi, dokumentasi, dan wawancara mendalam. Hasil penelitian menunjukkan bahwa praktik ini telah menjadi kebiasaan di Desa Balukang, di mana pupuk dan benih diberikan pada awal akad dengan pembayaran setelah masa panen dalam bentuk beras. Perjanjian dilakukan secara lisan dan dicatat oleh kreditur tanpa adanya perjanjian tertulis. Praktik ini seharusnya dianggap sebagai akad jual beli dengan pembayaran ditangguhkan, meskipun masyarakat umumnya mengenalnya sebagai utang piutang. Dari perspektif fikih muamalah, praktik ini diperbolehkan karena sudah menjadi kebiasaan yang tidak merugikan pihak manapun dan telah disepakati oleh kedua belah pihak.
Implementasi Prinsip Ultimum Remedium Terhadap Keterlanjuran Kegiatan Perkebunan Kelapa Sawit di Dalam Kawasan Hutan Berdasarkan Undang-Undang Cipta Kerja
Authors: Syafira Almutahaliya  
Published: January 2025

The provision in Article 37 number 20 of the Cipta Kerja, regulates the Ultimum Remedium Principle against the continuation of oil palm plantation activities which is reflected in the insertion of Articles 110A and 110B between Article 110 and Article 111 of Law Number 18 of 2013 concerning Prevention and Eradication of Forest Destruction. This research will answer the problem of how the implementation of the Ultimum Remedium Principle against the continuation of oil palm plantation activities in forest areas based on the Job Creation Law and how are the obstacles in the Implementation of the Ultimum Remedium Principle against the continuation of oil palm plantation activities in forest areas by the West Sumatra Provincial Forestry Service. The research method used is empirical legal research using primary data obtained by interviewing the Forest Damage Control and Security Section in the Forest Protection and Conservation of Natural Resources and Ecosystems Division of the West Sumatra Provincial Office. Based on the results of the research, the application of the ultimum remedium principle to the continuation of oil palm plantation activities in forest areas is resolved by applying for a permit by paying forest resource provisions and reforestation funds to the state treasury. Then for everyone who lives around or in the forest area for five consecutive years, can be subject to exclusion or maximum subject to administrative sanctions in the form of temporary suspension of business activities, payment of administrative fines or government coercion. Then the obstacles to the implementation of the ultimum remedium principle against the continuation of oil palm plantation activities in forest areas are in the form of technical problems in the field such as, lack of public understanding, uncooperativeness of authorized officials, and misappropriation by law enforcement officers.
Psychotypes of the elderly in the course of interaction with social workers
Authors: Budyakova Tatyana Petrovna 
Published: January 2025

The subject of the research is the study of personality types in old age. The relevance of the work is determined by the need to identify and describe the personality types of older people who exercise their right to receive social assistance from the state. This is important because the effectiveness of social support for the elderly depends on the targeting of its provision. At the same time, only general types of elderly people are traditionally studied, without regard to the factor of an organized form of social work. There are no typologies of elderly people seeking help from government agencies designed for the purposes of social assistance and protection. Taking into account the identity of the client of social services is of paramount importance for the full satisfaction of the diverse needs of an elderly person, including respect, however, technologies for such accounting have not been developed. A focus group interview was used as the main research method. The focus group included psychologists working in the center of social protection of the population and other specialists of this service. A case method was associated with the main method. The materials obtained using the case method were used in the formulation of typologies of the elderly. The scientific novelty of the study consists in identifying and describing three types of personality typologies in old age. We are talking about universal, particular and situational typologies. It was found that for the purposes of customer service in social protection centers, situational typologies are most effective, taking into account the nature of the situation in which work with an elderly client takes place. Typologies were described for three typical situations of social interaction with the elderly: the provision of humanitarian assistance, the provision of social services at home and the organization of communication groups based on the center for social protection of the population. A special contribution of the author is to establish the need to take into account the type of behavior and, accordingly, the type of personality in old age, when interacting with older people.
The connection between esotericism and science, or how the second "parasitizes" the first. Science as a reproducible quantum entanglement of the real trajectory and trajectory of thinking
Authors: Strigin Mikhail Borisovich 
Published: January 2025

The paper shows that esotericism is the area of bifurcation of knowledge in which new trajectories of thinking are born. Further, in accordance with the principle of Occam's Razor, some of them are eliminated as not corresponding to the principle of least action. The rest move into the field of regular science. It is shown that our world is a system of nested resonators, which is consistent with Descartes' idea that the world is built from a hierarchy of vortices. This also explains the great importance of boundary conditions in mathematics, since the boundaries of the resonator define the shape of the vortex. At the same time, the discreteness of energy, which is a characteristic feature of all quantum processes, becomes clear, since only isolated structures can persistently exist in the resonator. The general wave function of an entity is proposed as the product of a number of wave functions corresponding to each scale of the Universe. A model is proposed in accordance with which knowledge becomes scientific when the trajectory of thinking and the real trajectory of the system correlate with each other. A model is proposed in accordance with which knowledge becomes scientific when the trajectory of thinking and the real trajectory of the system correlate with each other. This correlation is built using mathematical signs. Since both the real trajectory and the trajectory of thinking are cyclical, their synchronization indicates the quantum entanglement of the real and the symbolic. The Jungian concept of synchronicity is being clarified. At any moment, by explicating a sequence of signs, we reproduce reality. All particles are entangled with each other to varying degrees. Therefore, the task of the experimenter is not to create entanglement, but rather to disentangle the object of study so that it can be studied autonomously.
Lipodistrofia asociada a antirretrovirales en personas que viven con el VIH: Prevalencia y factores asociados
Authors: Flores Juárez Emmanuel , Palacios Macedo Andrés Méndez , Robles Aguirre Bernardo Adrián 
Published: January 2025

Objetivo: determinar la prevalencia de lipodistrofia y factores asociados en un grupo de personas que viven con el virus de inmunodeficiencia humana con tratamiento antirretroviral, a través de evaluación del estado de nutrición para la implementación de estrategias de intervención adecuadas que permitan la mejora de su condición de salud. Materiales y métodos: Estudio observacional con muestra de 30 personas de origen mexicano del sexo masculino atendidos en los servicios médicos de Infectología del HGR No. 1 del IMSS “Lic. Ignacio García Téllez” y CAPASITS. Se estableció el diagnóstico de lipodistrofia matemáticamente a través de la relación masa grasa tronco (kg) / miembros inferiores (kg) > 1.5. Resultados: Se encontró que el 87.5% de la población estudiada presentaba lipodistrofia, con una proporción media de grasa tronco a extremidades inferiores de 1.56. El análisis de regresión logística reveló que el nivel de actividad física fue el único factor predictor significativo de la lipodistrofia (W= 9.114, p=0.03, OR= 18; IC95%: 2.756-117.554). Además, se observaron diferencias significativas en los niveles de glucemia en ayunas y recuento de linfocitos T CD4+ entre individuos con y sin lipodistrofia. Sin embargo, no se encontraron diferencias significativas en los niveles de colesterol ni en las horas de sueño. Se estableció una relación moderada entre el recuento de CD4+ y la relación masa grasa tronco/piernas (p= 0.011). No se encontró una asociación significativa entre el tipo de terapia farmacológica y la presencia de lipodistrofia (p= 0.154). Estos hallazgos sugieren la importancia de la actividad física en la prevención de la lipodistrofia en pacientes bajo tratamiento antirretroviral. Conclusiones: Estos hallazgos subrayan la importancia de implementar estrategias de promoción de la actividad física para la prevención y manejo de la lipodistrofia en pacientes con VIH bajo tratamiento antirretroviral.