bluuebunny/crossref_metadata_2025_split huggingface dataset:
An apical membrane complex for triggering rhoptry exocytosis and invasion in Toxoplasma
Authors: Sparvoli Daniela , Delabre Jason , Penarete‐Vargas Diana Marcela , Kumar Mageswaran Shrawan , Tsypin Lev M , Heckendorn Justine , Theveny Liam , Maynadier Marjorie , Mendonça Cova Marta , Berry‐Sterkers Laurence , Guérin Amandine , Dubremetz Jean‐François , Urbach Serge , Striepen Boris , Turkewitz Aaron P , Chang Yi‐Wei , Lebrun Maryse 
Published: October 2022

AbstractApicomplexan parasites possess secretory organelles called rhoptries that undergo regulated exocytosis upon contact with the host. This process is essential for the parasitic lifestyle of these pathogens and relies on an exocytic machinery sharing structural features and molecular components with free‐living ciliates. However, how the parasites coordinate exocytosis with host interaction is unknown. Here, we performed a Tetrahymena‐based transcriptomic screen to uncover novel exocytic factors in Ciliata and conserved in Apicomplexa. We identified membrane‐bound proteins, named CRMPs, forming part of a large complex essential for rhoptry secretion and invasion in Toxoplasma. Using cutting‐edge imaging tools, including expansion microscopy and cryo‐electron tomography, we show that, unlike previously described rhoptry exocytic factors, TgCRMPs are not required for the assembly of the rhoptry secretion machinery and only transiently associate with the exocytic site—prior to the invasion. CRMPs and their partners contain putative host cell‐binding domains, and CRMPa shares similarities with GPCR proteins. Collectively our data imply that the CRMP complex acts as a host–molecular sensor to ensure that rhoptry exocytosis occurs when the parasite contacts the host cell.
VALIDATION OF ASSESSMENT INSTRUMENTS FOR INTEGRATED SCIENCE LEARNING ON THE ABILITY OF STUDENT USING RASCH MODEL
Authors: Kristyasari Marantika Lia , Yamtinah Sri 
Published: October 2022

The Computerized Two Tier Multiple Choice (CTTMC) instrument has been created to mesure the bility of student in Integrated Science learning. This research was conducted to produce empirical evidence regarding the validity of the TTMC instrument using the Rasch Model analysis thorugh the Winstep program. A total of 283 junior high school students in the city of Surakarta were the research subjects. This assessment instrument consists of 20 questions. Statistical analysis was performed using the Rasch model. The results of the analysis show that in general, this assessment instrument can explain 40.7% of the variance that appears in each group of respondents. As many as 85% of the questions were declared fit and 15% of the questions still needed to be improved. Of the 283 participants, 13,4% had an ability misfit, 13,8% were included in the upper outlier, and 72,8% of the participants had an infit ability. Thus, that the assessment of Integrated Science SMP learning, in general, can be carried out using this instrument. How To Cite: Kristyasari, M.L., Yamtinah, S. (2022). Validation Of Assessment Instruments For Integrated Science Learning On The Ability Of Student Using Rasch Model. EDUSAINS, 14 (1) : 24-33.
DEVELOPMENT OF MEDIA CARD GAME VEROES (VERTEBRATES NEURO SYSTEM) FOR TRAINING REASONING SKILLS OF SMA STUDENTS ON NERVOUS SYSTEM MATERIALS
Authors: Nugroho Jefri , Ramli Murni 
Published: October 2022

This research was conducted because of the difficulties of students in learning the material on the nervous system. This research aims to produce Veroes Card Game learning media to train students' reasoning skills. This research is a mix method research carried out in two stages, namely the media preparation stage and the media feasibility test stage. The subjects used in the feasibility test of the media were public and islamic high school teachers in Sukoharjo regency and students majoring in biology and science education. The data collection technique is done by the feasibility test of the media using a questionnaire instrument. Data analysis of the feasibility test results were analyzed by Analysis of the Rasch Model using Winstep application. From the results of data analysis the instrument item reliability value obtained was 0.92, the respondent's reliability value was 0.89 and the Cronbach Alpha value was 0.93 so that it could be stated in very good category. The probability value of instrument items has a range of values above 5% (0.05) with three instrument items detected contain bias. Based on the research results, it can be stated that the Card Game Veroes media has met the criteria as a suitable learning medium to use in the learning process on the nervous system material.PENGEMBANGAN MEDIA CARD GAME VEROES (VERTEBRATES NEURO SYSTEM) UNTUK MELATIH REASONING SKILLS SISWA SMA PADA MATERI SISTEM SARAFAbstrakPenelitian ini dilaksanakan karena adanya kesulitan siswa dalam mempelajari materi sistem saraf. Penelitian bertujuan untuk menghasilkan media pembelajaran Card Game Veroes untuk melatih reasoning skills siswa. Penelitian ini merupakan penelitian mix method yang dilaksanakan dengan dua tahap, yaitu tahap penyusunan media dan tahap uji kelayakan media. Subjek yang digunakan dalam uji kelayakan media adalah Guru SMA/MA wilayah Kabupaten Sukoharjo dan mahasiswa jurusan pendidikan biologi dan IPA. Teknik pengumpulan data dilakukan dengan uji kelayakan terhadap media menggunakan instrumen angket. Analisis data hasil uji kelayakan dianalisis menggunakan Analisis Model Rasch dengan aplikasi Winstep. Dari hasil analisis data diperoleh nilai reliabilitas item instrumen sebesar 0,92, nilai realibilitas responden sebesar 0,89 dan nilai Alpha cronbach sebesar 0,93 sehingga dapat dinyatakan dalam kategori sangat bagus. Nilai probabilitas item instrumen memiliki kisaran nilai diatas 5% (0,05) dengan tiga item instrumen yang terdeteksi mengandung bias.Berdasarkan hasil penelitian, dapat dinyatakan media Card Game Veroes telah memenuhi kriteria sebagai media pembelajaran yang layak untuk digunakan dalam proses pembelajaran pada materi sistem saraf.How To Cite: Nugroho,, J., Ramli, M. (2022). Pengembangan Media Card Game VEROES (Vertebrates Neuro System) Untuk Melatih Reasoning Skills Siswa SMA Pada Materi Sistem Saraf. EDUSAINS, 14 (1) : 63-71
THE DEVELOPMENT OF A CHEMOENTREPRENURSHIP (CEP) ORIENTED COLLOID CHEMISTRY E-MODULE ASSISTED BY 3D PAGE FLIP PROFESSIONAL
Authors: Herdini Herdini , Erna Maria , Indah Nursya Alma 
Published: October 2022

The development of a chemoentrepreneurship (CEP) oriented Colloidal Chemistry E-Module assisted by 3D Page Flip Professional (3D PFP) is important to with produce students teaching material products that are related to their daily life environment and can stimulate students to create economically valuable and independent products. . To produce a very valid E-Module product, the user response is very good and it looks very attractive, research and development (R&D) is carried out with a 4-D model (Define, Design, Develop and Disseminate). The results of the validity of material and media experts on the developed E-Module are 95.54% and 95.5%, respectively, with a very valid category. The results of the limited test, namely a one-on-one test on 3 students, obtained information that the E-Module needed to be revised. While the user test results from 3 chemistry teachers obtained an average questionnaire score of 94% with very good criteria. Then the results of a limited test of 30 students obtained an average score of 88% with very interesting criteria. The resulting CEP-oriented E-Module products assisted by 3D PFP are expected to increase students' entrepreneurial interest.PENGEMBANGAN E-MODUL KIMIA KOLOID BERORIENTASI CHEMOENTREPRENURSHIP (CEP) BERBANTUAN 3D PAGE FLIP PROFESSIONAL AbstrakPengembangan E-Modul Kimia Koloid berorientasi chemoentrepreneurship (CEP) berbantuan 3D Page Flip Professional (3D PFP) penting dilakukan untuk menghasilkan peserta didik produk bahan ajar yang berhubungan dengan lingkungan kehidupan sehari-hari dan dapat menstimulus peserta didik untuk membuat produk bernilai secara ekonomis dan mandiri. Untuk menghasilkan produk E-Modul yang sangat valid, respon pengguna sangat baik dan tampilannya sangat menarik dilakukan penelitian dan pengembangan (R&D) dengan model 4-D (Define, Design, Develop dan Disseminate). Hasil validitas ahli materi dan media terhadap E-Modul yang dikembangkan berturut-turut adalah 95,54% dan 95,5% dengan kategori sangat valid. Hasil uji terbatas yaitu uji satu-satu terhadap 3 orang peserta didik diperoleh informasi bahwa E-Modul perlu direvisi. Sedangkan hasil uji pengguna dari 3 orang guru kimia diperoleh skor rata-rata angket sebesar 94% dengan kriteria sangat baik. Kemudian hasil uji terbatas terhadap 30 orang peserta didik diperoleh skor rata-rata angket sebesar 88% dengan kriteria sangat menarik. Produk E-Modul berorentasi CEP berbantuan 3D PFP yang dihasilkan diharapkan dapat meningkatkan minat kewirausahaan peserta didik.How To Cite: Herdini, Erna, M., Indah, N.A. (2022). Pengembangan E-Modul Kimia Koloid Berorientasi Chemoentreprenurship (CEP) Berbantuan 3D Page Flip Professional. EDUSAINS, 14 (1) : 50-62.
Mensch und Tier, Sprache und Politik bei Aristoteles
Authors: Simon Attila 
Published: October 2022

AbstractThe paper aims to clarify connections among the terms designated in the title on the basis of a well-known passage of the Aristotelian corpus (Politics I 2. 1253a7–18). The first part of the argumentation unfolds mainly through conceptual explanations and conceptual distinctions, during which some relevant claims of Aristotleʼs other works (primarily the biological and logical writings) are also taken into consideration. The main question of this part is directed to the difference between man and other living beings. The difference, as it will appear, lies in the specific performance of human speech (logos), contrasted with the performance of the animal voice (phōnē). In the second part, the reconstruction of Aristotelian theory based on close textual reading is complemented by references to some later but theoretically insightful contexts, especially with regard to the field of politics in the narrower sense. Conceptions of social constructivism (Berger and Luckmann), philosophical anthropology (Gehlen), political philosophy (Arendt), and philosophical hermeneutics (Heidegger and Gadamer) will primarily come into play.
Relationship between adverse childhood experiences and problematic internet use among young adults: The role of the feeling of loneliness trajectory
Authors: Lin Wen-Hsu , Chiao Chi 
Published: October 2022

AbstractBackgrounds and aimsGiven problematic Internet usage's (PIU) negative impact on individual health, this study evaluates how adverse childhood experiences (ACEs) affect young adults' PIU and the possible underlying mechanism of the “feeling of loneliness” (FOL) trajectory.MethodsAnalyzing a retrospective cohort sample from the Taiwan Youth Project, 2,393 adolescents were interviewed from the average ages of 14–28. We constructed ACE in 2000 using six categories (e.g., abuse and low family socioeconomic status) and 5-item PIU in 2017 from Chen's Internet Addiction Scale. FOL trajectories measured eight times, at average ages 14, 16, 17, 18, 20, 22, 25, 28 years-old.ResultsOverall, 12.65% of the participants did not have ACEs, and 12.78% exhibited PIU. FOL trajectory analyses yielded three groups: “constant low” (reference group: 53.25%); “moderate decline” (36.81%); and “increasing” (9.94%). Regression models showed a dose–response association between ACE and young adults' PIU (adjusted odds ratio = 1.12; 95% confidence interval [CI] = 1.02–1.23) and the two risky loneliness groups (moderate decline: relative risk ratio [RRR] = 1.42, 95% CI = 1.32–1.54; increasing: RRR = 1.52, 95% CI = 1.37–1.71). Structural equation modeling further found that ACEs increase young adults' risk of being in the increasing group, and consequently, the risk of PIU.Discussion and conclusionsWe demonstrated that ACE may be associated with 1) adults' PIU, 2) FOL from adolescence to emerging adulthood, and 3) young adults' PIU through its association with FOL trajectories.
Non-destructive evaluation of steel rebars corrosion in concrete beams
Authors: Elmennaouy Fatima , Mouhat Ouadia 
Published: October 2022

Abstract
The present study aims at developing a non-destructive evaluation technique based on elastic wave scattering and the finite element method for the detection and evaluation of localized damage in rebar of reinforced concrete beams. Simulation of corrosion effect was taken for a tensile bar by creating a defect in this bar while the rest of the rebar and stirrups were kept unchanged. The study is based on comparison of propagation pattern of elastic waves in the presence and in the absence of defect. The proposed method was found capable to provide non-destructive evaluation of rebar corrosion in reinforced concrete beams, thus enabling diagnosis of reinforcement concretes structures attacked by corrosion. The obtained results can be further used to locate corroded areas.
Nanomaterials in diagnostics, imaging and delivery: Applications from COVID-19 to cancer
Authors: Bardhan Neelkanth 
Published: October 2022

Abstract
In the past two decades, the emergence of nanomaterials for biomedical applications has shown tremendous promise for changing the paradigm of all aspects of disease management. Nanomaterials are particularly attractive for being a modularly tunable system; with the ability to add functionality for early diagnostics, drug delivery, therapy, treatment and monitoring of patient response. In this review, a survey of the landscape of different classes of nanomaterials being developed for applications in diagnostics and imaging, as well as for the delivery of prophylactic vaccines and therapeutics such as small molecules and biologic drugs is undertaken; with a particular focus on COVID-19 diagnostics and vaccination. Work involving bio-templated nanomaterials for high-resolution imaging applications for early cancer detection, as well as for optimal cancer treatment efficacy, is discussed. The main challenges which need to be overcome from the standpoint of effective delivery and mitigating toxicity concerns are investigated. Subsequently, a section is included with resources for researchers and practitioners in nanomedicine, to help tailor their designs and formulations from a clinical perspective. Finally, three key areas for researchers to focus on are highlighted; to accelerate the development and clinical translation of these nanomaterials, thereby unleashing the true potential of nanomedicine in healthcare.

Graphical abstract
"Argel fingido y renegado de amor". Lope entre Garcilaso y Ariosto
Authors: Serés Guillermo 
Published: October 2022

Lope recrea metateatralmente los principales tópicos o arquetipos de la
literatura de su tiempo: el preso, el pastor, el peregrino, el náufrago de amor y el enloquecido de amor de raíz ariostesca, que, a su vez, convoca a otras figuras míticas afines, como las de Leandro o Ícaro, también muy caracterizadas. Una vez resuelto el enredo, hechos los pertinentes exámenes de conciencia y liberados de los insidiosos afectos y desviadas conductas, todos los personajes abandonan su disfraz -incluido el renegado Rosardo- y se disponen a amar rectamente.
Differentiation and subpopulation composition of VEGFR2+ cells in the blood and bone marrow in ischemic cardiomyopathy
Authors: Chumakova S. P. , Urazova O. I. , Shipulin V. M. , Denisenko O. A. , Kononova T. E. , Nevskaya K. V. , Andreev S. L. 
Published: October 2022

Aim. To identify disturbances of differentiation and subpopulation composition of VEGFR2+ cells in the blood and bone marrow associated with the features of the cytokine profile in the blood and bone marrow in patients with coronary artery disease (CAD) with and without ischemic cardiomyopathy (ICM).Materials and methods. The study included 74 patients with СAD with and without ICM (30 and 44 people, respectively) and 18 healthy donors. In all patients with СAD, peripheral blood sampling was performed immediately before coronary artery bypass grafting, and bone marrow samples were taken during the surgery via a sternal incision. In the healthy donors, only peripheral blood sampling was performed. In the bone marrow and blood samples, the number of VEGFR2+ cells (CD14+VEGFR2+ cells) and their immunophenotypes CD14++CD16-VEGFR2+, CD14++CD16+VEGFR2+, CD14+CD16++VEGFR2+, and CD14+CD16-VEGFR2+ was determined by flow cytometry. Using enzyme-linked immunosorbent assay, the levels of VЕGF-А, TNFα, M-CSF, and IL-13, as well as the content of MCP-1 (only in the blood) and the M-CSF / IL-13 ratio (only in the bone marrow) were determined.Results. The content of CD14+VEGFR2+ cells in the blood of CAD patients with and without ICM was higher than normal values due to the greater number of CD14++CD16-VEGFR2+, CD14++CD16+VEGFR2+, and CD14+CD16++VEGFR2+. In the bone marrow of the patients with ICM, the content of CD14++CD16-VEGFR2+, CD14+CD16++VEGFR2+, and CD14+CD16-VEGFR2+ was lower than in patients with CAD without ICM, and the number of CD14++CD16+VEGFR2+ cells corresponded to that in the controls. Regardless of the presence of ICM in CAD, a high concentration of TNFα and normal levels of VEGF-A and IL-13 were observed in the blood. In CAD without ICM, an excess of MCP-1 and deficiency of M-CSF were revealed in the blood. In the bone marrow, the levels of VEGF-A, TNFα, M-CSF, and IL-13 were comparable between the groups of patients against the background of a decrease in the M-CSF / IL-13 ratio in the patients with ICM.Conclusion. Unlike CAD without cardiomyopathy, in ICM, no excess of VEGFR2+ cells and MCP-1 in the blood is observed, which hinders active migration of CD14+CD16++VEGFR2+ cells from the myeloid tissue, and a decrease in the M-CSF / IL-13 ratio in the bone marrow disrupts differentiation of other forms of VEGFR2+ cells, preventing vascular repair.
Morphological changes in the heart and aorta of rats with diet-induced metabolic syndrome
Authors: Birulina J. G. , Ivanov V. V. , Buyko E. E. , Bykov V. V. , Dzyuman A. N. , Nosarev А. V. , Grigoreva A. V. , Gusakova S. V. 
Published: October 2022

Aim. To identify early morphological changes in the heart and aorta of rats with experimental metabolic syndrome induced by a high-fat and high-carbohydrate diet (HFHCD).Materials and methods. The study was carried out on male Wistar rats. The animals were divided into two groups: a control group (n = 10) and an experimental group (n = 10). The rats from the control group were fed with a standard laboratory diet. The rats from the experimental group received HFHCD for 12 weeks. Body weight, blood pressure (BP), and individual parameters of carbohydrate and lipid metabolism were assessed in the rats. A histologic examination of the heart and aorta in the animals was performed.Results. Feeding rats with HFHCD led to an increase in body weight, elevation of BP, obesity, hyperglycemia, and triglyceridemia. The histologic examination of the heart in the rats of the experimental group showed signs of vascular disease, lipomatosis, and focal myocardial degeneration. Lipid accumulation in the cells of the media, hyperplasia of adipocytes in the adventitia, and depletion and fragmentation of the elastic lamina were revealed in the aortic wall of the rats receiving HFHCD.Conclusion. The study indicated that HFHCD is an effective way to model metabolic syndrome. Structural disorders in the heart and aorta may be the mainstay for the development of cardiomyopathy and arterial hypertension in diet-induced metabolic syndrome.
Radionuclide methods in assessing pulmonary perfusion and ventilation in patients with connective tissue dysplasia
Authors: Vesnina Zh. V. , Anashbaev Zh. Zh. , Teteneva A. V. , Krivinogov N. G. , Bespalova I. D. , Sazonova S. I. , Serdyukov N. A. , Potapov K. V. 
Published: October 2022

Aim. To perform a scintigraphic assessment of the bronchopulmonary system and pulmonary microcirculation in patients with connective tissue dysplasia.Materials and methods. The study included 31 male patients of conscription age with connective tissue dysplasia (CTD), diagnosed according to the 2019 clinical guidelines (average age (19.6 ± 2.6 years)), and 25 practically healthy individuals comparable in gender and age, who formed a control group. All patients underwent planar pulmonary ventilation – perfusion scintigraphy with determination of pulmonary alveolar – capillary permeability.Results. In patients with CTD, the apical to basal perfusion gradient (U/LQ) was on average 24% lower than in the control group (p = 0.046), and alveolar – capillary permeability was higher in both lungs, both at minute 10 and at minute 30.Conclusion. Static pulmonary ventilation – perfusion scintigraphy allows to identify functional disorders in patients with CTD at the preclinical stage: a decrease in the perfusion gradient on average by 24% compared with the control group and an increase in alveolar – capillary membrane permeability.
Lung cancer in patients with COPD and factors associated with reduced survival
Authors: Dobner S. Yu. , Fedosenko S. V. , Rodionov E. O. , Yarovoy N. D. , Petrov V. A. , Tuzikov S. A. , Starovoitova E. A. , Samykina I. A. 
Published: October 2022

Background. A combination of different types of lung cancer and chronic obstructive pulmonary disease (COPD) is very common. COPD, accompanied by ventilation disorders and, often, respiratory failure, is a significant additional risk factor for mortality in these patients. Identification of risk factors for mortality in patients with lung cancer and COPD can potentially be associated with better long-term outcomes.Materials and methods. Using a Cox regression model based on information about the outcome of the disease and life expectancy after treatment initiation, a survival analysis was performed with an assessment of the contribution of various clinical and anamnestic factors for a group of 118 COPD patients with primary diagnosed lung cancer. These patients received treatment at the Cancer Research Institute in Tomsk in 2013–2019.Results. The study included 118 patients (87.3% men and 12.7% women). Among them, 77.97% of patients were active or former smokers with smoking index (SI) ≥ 10 pack-years, and 22% of patients had never smoked or had SI < 10 pack-years but had other risk factors for COPD. Peripheral lung cancer was detected in 45.8% of cases. Squamous cell carcinoma was noted in 54.2% of cases, adenocarcinoma – in 34.7%, large cell carcinoma – in 1.7%, small cell carcinoma – in 5.9%, and carcinoid tumors – in 2.5% of cases. Patients were characterized by varying degrees of severity of ventilation disorders in accordance with the GOLD classification: stage 1 was observed in 44% of patients, stage 2 – in 38.1 % of patients, stage 3 – in 16.9 % of patients, and stage 4 – in one patient. Threeyear mortality was 28.12%.Conclusion. According to the results of the Cox regression analysis, factors that significantly reduced the survival rate of patients with lung cancer in combination with COPD were more severe stages in terms of the size of the primary tumor and its localization, the prevalence of metastasis (according to TNM classification), more severe dyspnea (mMRC scale), lower oxygen saturation values, atelectasis, and episodes of pneumonia, including paracancrotic pneumonia, over the previous 12 months. The presence of certain types of metastases, such as metastatic lesions of the pleura, adrenal glands, distant non-regional lymph nodes, and bones should also be noted as negative factors for survival. It is worth noting that surgical treatment of the primary tumor was associated with an increase in the survival rate in patients with lung cancer in combination with COPD.