bluuebunny/crossref_metadata_2025_split huggingface dataset:
Florida Invader: Cane Toad
Authors: Johnson Steve A. , McGarrity Monica E. 
Published: February 2022

Do you know how to tell the difference between a dangerous Cane Toad (a.k.a. “Bufo” Toad or Marine Toad) and a harmless native toad? This 2-page fact sheet will help you correctly identify and humanely euthanize this invasive toad that can kill pets. It was written by Steve Johnson and Monica McGarrity and published by the UF Department of Wildlife Ecology and Conservation, November 2010.
2010 PDF version unavailable, 2014 version included instead.
A biztonságtudatosítás pszichés gátjai: szubjektív veszély- és kontrollpercepció a digitális térben
Authors: Héder Klára 
Published: February 2022

Az információbiztonság-tudatosítás sikere össztársadalmi érdek. E hosszú távú folyamat eredményességének biztosításához mind a programok készítői oldalán, mind pedig a célcsoporti oldalon fontos, hogy megismerjük és kivédjük azokat a gátakat, amelyek a kimeneti eredményességet befolyásolhatják. A cikk bemutatja azokat a kérdéseket, amelyeket az információbiztonság-tudatosítás „technokrata megközelítése” vet fel. Bemutatja a képzések kialakítása mögött megbújó rejtett feltételezéseket, a felhasználói oldalon tapasztalható pszichés hárítások és ellenállási pontok mögött húzódó fontosabb okokat, a szubjektív veszély- és kontrollpercepció hatását a programokban való hosszú távú részvételi szándékra.
FAKTOR-FAKTOR YANG MEMENGARUHI BESARNYA ANGKA RUJUKAN PASIEN PESERTA BADAN PENYELENGGARA JAMINAN SOSIAL (BPJS) DI PUSKESMAS SINDANG BARANG KOTA BOGOR TAHUN 20202
Authors: Dwi Permata Elok , Khodijah Parinduri Siti , Fathimah Ratih 
Published: February 2022

Rujukan merupakan penyerahan tanggung jawab secara timbal balik atas timbulnya masalah atau kasus masalah kesehatan masyarakat, baik secara vertikal dalam arti satu strata sarana pelayanan kesehatan ke strata pelayanan kesehatan lainnya, maupun secara horizontal dalam arti antara sarana pelayanan kesehatan yang sama, kepada yang lebih kompeten, terjangkau, dan dilakukan secara rasional. Tujuan penelitian ini adalah untuk mengetahui hubungan antara faktor predisposisi, faktor penguat dan faktor pemungkin dengan besarnya rujukan di puskesmas Sindang Barang Kota Bogor. Jenis penelitian ini menggunakan metode penelitian kuantitatif dengan jenis rancangan Cross Sectional. Responden dipilih secara random sampling sebanyak 110 responden dengan kuesioner. Uji statistic yang digunakan adalah Chi Square. Hasil penelitian menunjukan bahwa tidak ada hubungan antara pengetahuan (p= 0,413), sarana dan SDM (p= 0,839), usia (p= 0,121), pendidikan (p= 0,520) dan jenis kelamin (p= 0,223). Sedangkan informasi tentang rujukan (p= 0,000) menunjukan bahwa ada hubungan yang signifikan dengan rujukan. Kesimpulan akhir peneliti ini adalah pasien yang kekurangan informasi tentang rujukan dapat mengakibatkan pasien tersebut cenderung lebih memilih mendapatkan layanan sekunder karena memiliki kepercayaan diri yang rendah dalam menacri pengobatan di puskesmas.
FAKTOR-FAKTOR PEMANFAATAN PELAYANAN KESEHATAN DI PUSKESMAS PARUNG SELAMA MASA PANDEMI COVID-19
Authors: Mardiana Nena , Chotimah Indira , Dwimawati Eny 
Published: February 2022

Penurunan kunjungan pasien pada Puskesmas Parung pada tahun 2020 disebutkan sebanyak 70,65% masyarakat yang tidak memanfaatkan pelayanan kesehatatan. kapasitas puskesmas dalam memberikan layanan mengalami penurunan baik pada Upaya Kesehatan Masyarakat (UKM) dan upaya kesehatan perorangan (UKP). Metode yang digunakan merupakan metode penelitian kuantitatif dengan jumlah sampel sebanyak 130 responden menggunakan teknik accidental sampling. Instrumen yang digunakan dalam penelitian ini adalah kuesioner, data yang telah dikumpulkan selanjutnya diolah dan dianalisis dengan analisis data univariat, bivariat dan multivariat. Hasil penelitian menunjukkan bahwa sebanyak 130 responden diantaranya 27 responden (20,8%) jarang memanfaatkan pelayanan kesehatan dan 103 responden (79,2%) sering memanfaatkan pelayanan kesehatan. Berdasarkan uji bivariat dalam penelitian ini umur (p=0,635), pendidikan (p=0,047), pekerjaan (p=0,454), aksesibilitas (p=0,006) dan pelayanan dokter (p=0,016), Ada hubungan terhadap pemanfaatan pelayanan kesehatan berdasarkan uji multivariat pada variabel pendidikan, aksesibilitas dan pelayanan dokter. Berdasarkan uji multivariat variabel aksesibilitas mempunyai nilai Exp (B) sebesar 0,269 dan variabel pelayanan dokter mempunyai nilai Exp (B) 0,282, merupakan model terbaik untuk menentukan determinan terhadap pemanfaatan pelayanan kesehatan di Puskesmas Parung.Peneliti dapat mengambil kesimpulan bahwa tingkat pendidikan,aksesbilitas dan pelayanan dokter memiliki hubungan yang signifikan terhadap pemanfaatan pelayanan kesehatan yang mana pelayanan dokter memiliki pengaruh yang besar. Saran dari peneliti kepada Puskesmas Parung agar terus meningkatkan dan mempertahankan pelayanan kesehatan yang tersedia serta lebih memperhatikan protokol kesehatan
Analisis Tentang Pengembilan Keputusan Dalam Memilih Program Prodi Pendidikan Nonformal
Authors: Muthia Muthia , Zulkarnain Rufran 
Published: February 2022

Tujuan dari penelitian ini adalah untuk mengetahui seberapa besar pengaruh faktor orang tua, faktor teman sebaya, faktor citra perguruan tinggi, dan faktor prospek pekerjaan dalam pengambilan keputusan memilih program studi Pendidikan Nonformal FKIP Universitas Bengkulu. Jenis penelitian ini adalah Kuantitatif. Penelitian ini dilakukan dengan metode analisis deskriptif dan analisis faktor, sedangkan yang menjadi informan penelitian adalah mahasiswa Prodi Pendidikan Nonfromal FKIP UNIB angkatan 2017-2020 berjumlah 53 mahasiswa. Teknik pengumpulan data menggunakan kuesioner yang disebar pada mahsiswa PNF angkatan 2017-2020. Hasil penelitian menunjukkan bahwa Faktor orang tua memiliki tingkat pengaruh sebesar 70,26 % ,faktor teman sebaya memiliki tingkat pengaruh sebesar 54 %, faktor citra perguruan tinggi memiliki tingkat pengaruh sebesar 88,18%, dan faktor Prospek Pekerjaan dengan tingkat pengaruh sebesar 81,58 %.
Upaya Pemerintah Desa Dalam Penanggulangan Kenakalan Remaja Mengkosumsi Samsocid
Authors: Darwan Yogi , Rachmat Agus Zainal 
Published: February 2022

Penelitian ini bertujuan untuk mendapatkan informasi secara sistematis berkaitan dengan upaya Pemerintah Desa dalam penanggulangan kenakalan remaja mengkonsumsi Samcodin di Desa Padang Berangin. Metode yang digunakan Focus group discussion dengan mendapatkan hasil dari penelitian bahwa Upaya Pemerintah Desa Padang Berangin dalam penanggulangan kenakalan remaja dengan melaksanakan sosialisasi Hukum dan bahaya Narkotika supaya memberi pengetahuan kepada masyarakat desa terutama kalangan remaja dan membentuk Kader masyarakat guna membantu Pemerintah Desa Padang Berangin menanggulangi kenakalan remaja serta menghidupkan kembali organisasi kepemudaan yaitu karang taruna supaya membantu desa dan masyarakat dalam penanggulangan kenakalan remaja sesuai dengan fungsi karang taruna seperti meningkatkan nilai agama,ketakwaan dan akhlak mulia bagi remaja.
Case Series: Extracorporeal Shockwave Myocardial Revascularization Therapy Improves Ischemic Response, Functional Capacity, and Quality of Life in Indicated CABG-Stable Angina Pectoris Patients
Authors: Akbar Mohammad Rizki , Adiputro Dwi Laksono , Tiksnadi Badai Bhatara , Soeriadi Erwin Affandi , Hasan Melawati , Muttaqien Fauzan , Raharjo Pradana Pratomo , Nurazizah Eliza , Tarsidin Najmi Fauzan 
Published: February 2022

IntroductionExtracorporeal shockwave myocardial revascularization (ESMR) is included in the guidelines only for patients with refractory angina pectoris having no option for invasive revascularization. We intend to report a case series with ESMR therapy is indicated patients with coronary artery bypass grafting-stable angina pectoris (CABG-SAP) who refuse the surgery, irrespective of angina symptoms.MethodsWe review medical records of patients with SAP admitted to ESMR therapy in Dr. Hasan Sadikin General Hospital, Bandung, Indonesia from January 2018 to December 2019. Recorded variables at baseline and after therapy extracted, namely, (1) ischemic response, double product, and (2) functional capacity measured as metabolic equivalent (MET) using treadmill test; (3) six-minute walking test distance achieved; and (4) quality of life using SF-36 Questionnaire.ResultsA total of four indicated patients with CABG-SAP from 50 to 75 years old were included in this study. At baseline, one patient is CCS class I and two patients are CCS class II with SDS ranging from 3 to 17. Ischemic response improved in all the patients. The double product improved in patient 1 9,600–14,872 mm Hg × bpm, patient 2 9,460–10,640 mm Hg × bpm, and patient 4 17,220–20,480 mm Hg × bpm. The functional capacity improved in Patient 1 8.07–8.91 METs, patient 2 1.91–4.01 METs, patient 3 3.45–6.39 METs, and patient 4 3.9–4.43 METs. The 6-min walking distance improved in patient 1 540–570 m and patient 2 345–405 m. The CCS class, bodily pain, and general health domain scores improved in all patients.ConclusionESMR therapy might be beneficial for indicated patients with CABG-SAP to improve ischemic response, functional capacity, and physical component of quality of life.
Adipocytes-Derived Extracellular Vesicle-miR-26b Promotes Apoptosis of Cumulus Cells and Induces Polycystic Ovary Syndrome
Authors: Zhou Guannan , Gu Yuanyuan , Zhou Fangyue , Zhang Hongdao , Zhang Menglei , Zhang Ganrong , Wu Ligang , Hua Keqin , Ding Jingxin 
Published: February 2022

BackgroundPolycystic ovary syndrome (PCOS) is a refractory reproductive disease and also a kind of endocrine and metabolic disease. Adipocyte cells can produce a mass of extracellular vesicles and orchestrate the status of other types cells. The objective of this study was to determine the effects of adipocyte-derived extracellular vesicles-miR-26b on cumulus cells (CCs) and development of PCOS.MethodsThe crosstalk mediated by extracellular vesicle-miR-26b between adipocytes and CCs was determined in CC cells co-cultured with mature adipocytes or incubated with extracellular vesicle isolated from mature adipocytes. CCK-8 assay and flow cytometry were conducted in CCs treated with or without extracellular vesicles; microRNA (miRNA) sequencing was conducted for clarifying the key molecular. Hormone levels and ovary ovulation ability were conducted with animal experiment.ResultsThe results revealed that miR-26b was upregulated in extracellular vesicles derived from mature adipocytes. Adipocyte-derived extracellular vesicles inhibited viability and promoted apoptosis in CCs via targeting JAG1. Furthermore, extracellular vesicles derived from mature adipocyte disrupted the ovary ovulation and impaired the hormone levels.ConclusionsThese results identify a novel signaling pathway that adipocytes-derived extracellular vesicles-miR-26b promotes cell apoptosis in CCs and disrupted the ovary ovulation in the development of PCOS. The study indicates that adipose tissue-derived extracellular vesicles-miR-26b may play a key role in the PCOS and also provides insight into developing new therapeutic strategies for PCOS.
Systematic Understanding of Anti-Aging Effect of Coenzyme Q10 on Oocyte Through a Network Pharmacology Approach
Authors: Yang Liuqing , Wang Heng , Song SuJie , Xu Hongbin , Chen Yun , Tian Saisai , Zhang Yiqun , Zhang Qin 
Published: February 2022

BackgroundMaternal oocyte aging is strongly contributing to age-related decline in female fertility. Coenzyme Q10 (CoQ10) exerts positive effects in improving aging-related deterioration of oocyte quality, but the exact mechanism is unclear.ObjectiveTo reveal the system-level mechanism of CoQ10’s anti-aging effect on oocytes based on network pharmacology.MethodsThis study adopted a systems network pharmacology approach, including target identification, data integration, network and module construction, bioinformatics analysis, molecular docking, and molecular dynamics simulation.ResultA total of 27 potential therapeutic targets were screened out. Seven hub targets (PPARA, CAT, MAPK14, SQSTM1, HMOX1, GRB2, and GSR) were identified. Functional and pathway enrichment analysis indicated that these 27 putative targets exerted therapeutic effects on oocyte aging by regulating signaling pathways (e.g., PPAR, TNF, apoptosis, necroptosisn, prolactin, and MAPK signaling pathway), and are involved oxidation-reduction process, mitochondrion, enzyme binding, reactive oxygen species metabolic process, ATP binding, among others. In addition, five densely linked functional modules revealed the potential mechanisms of CoQ10 in improving aging-related deterioration of oocyte quality are closely related to antioxidant, mitochondrial function enhancement, autophagy, anti-apoptosis, and immune and endocrine system regulation. The molecular docking study reveals that seven hub targets have a good binding affinity towards CoQ10, and molecular dynamics simulation confirms the stability of the interaction between the hub targets and the CoQ10 ligand.ConclusionThis network pharmacology study revealed the multiple mechanisms involved in the anti-aging effect of CoQ10 on oocytes. The molecular docking and molecular dynamics simulation provide evidence that CoQ10 may act on these hub targets to fight against oocytes aging.
Establishment of Reference Intervals for Thyroid-Associated Hormones Using refineR Algorithm in Chinese Population at High-Altitude Areas
Authors: Ma Chaochao , Zhong Jian , Zou Yutong , Liu Zhijuan , Li Honglei , Pang Jinrong , Liu Xiaoxing , Zejipuchi  , Tian Liping , Hou Li’an , Wang Danchen , Cheng Xinqi , Qiu Ling 
Published: February 2022

ObjectivesDiagnosis of thyroid disease among individuals dwelling at high altitude remains a challenge. Reference intervals (RIs) for thyroid-associated hormones among Tibetans living at various high altitudes were established to improve diagnosis.MethodsOne thousand two hundred eighty-one subjects were randomly recruited from Nyingchi, Shigatse/Lhasa, and Ali of Tibet. Thyroid-stimulating hormone (TSH), free triiodothyronine (FT3), and free thyroxine (FT4) were measured by the Cobas e601 electrochemiluminescence analyzer. We used multiple linear regression and variance component analysis to assess the effect of sex, age, and altitude on hormones. RIs were established by refineR algorithm and compared with those provided by the manufacturer.ResultsSerum TSH was significantly lower in males than in females, while FT3 and FT4 were higher in males. Both FT3 and FT4 decreased with increasing age. FT3 increased with altitude, while TSH and FT4 were less influenced by altitude. The RI for TSH was 0.764–5.784 μIU/ml, while for FT4, the RIs were 12.36–19.38 pmol/L in females and 14.84–20.18 pmol/L in males. The RIs for FT3 at Nyingchi, Shigatse/Lhasa, and Ali in females were 4.09–4.98, 4.31–5.45, and 4.82–5.58 pmol/L, while in males, the values were 4.82–5.41, 4.88–5.95, and 5.26–6.06 pmol/L, respectively. The obtained RIs for TSH and FT4 were generally higher, while that for FT3 was narrower than the RIs provided by Cobas.ConclusionsSpecific RIs were established for thyroid-associated hormones among Tibetans, which were significantly different from those provided by the manufacturer.
Evaluation of Marginal Land Potential and Analysis of Environmental Variables of Jerusalem Artichoke in Shaanxi Province, China
Authors: Zhao Danyun , Yin Fang , Ashraf Tariq , Yuan Zheng , Ye Linxiao 
Published: February 2022

Based on the maximum entropy modeling (Maxent) and ArcGIS tool, this study assessed the potential of marginal land and analyzed the impact of environmental variables for Jerusalem artichoke (Helianthus tuberosus L.) in Shaanxi Province, China. The results showed that the dominant land type used for the growth of Jerusalem artichoke was moderately dense grassland. Additionally, significant environmental variables of Jerusalem artichoke and their suitable range in Shaanxi Province were average slope (SLP, 0–5°C), average soil depth (DPT, 1.50–1.60 m), max temperature of the warmest month (Bio5, 30–31°C), annual mean temperature (Bio1, 16.5–18.0°C), precipitation of the wettest quarter (Bio16, 0.01–0.02 m), July solar radiation (SR7, 1.66–1.67 × 107 W/m2), precipitation seasonality (Bio15, 50–60%), precipitation of the driest quarter (Bio17, 0–0.005 m), and isothermality (Bio3, 265–275). Furthermore, the suitable area was mainly distributed in southern (mainly Hanzhong, Ankang, and Shangluo) and northern (mainly Yan’an and Yulin) parts of Shaanxi Province, covering around 8.81 × 1010 m2 and accounting for 42.8% of the total area of the Shaanxi Province. This study can provide a reference for the rational planting of Jerusalem artichoke in Shaanxi Province.
Comprehensive Genomic and Epigenomic Analyses on Transcriptomic Regulation in Stomach Adenocarcinoma
Authors: Chen Junxing , Liu Weinan , Du Jiabin , Wang Pengcheng , Wang Jintian , Ye Kai 
Published: February 2022

Background: DNA methylation (MET)–mediated transcriptomic disturbance and copy number variations (CNVs) exert a significant influence in stimulating the heterogeneous progression of stomach adenocarcinoma (STAD). Nevertheless, the relation of DNA MET with CNVs, together with its impact on tumor occurrence, is still unclear.Methods: The messenger RNA (mRNA) expression (EXP) profiles, DNA MET, and DNA copy numbers, together with STAD mutation data, were collected from the TCGA official data portal. We employed circular binary segmentation algorithm in “DNAcopy.” library of R package for mapping DNA CNV data at genetic level for all samples based on the segmented CNV data. Stable clusters of samples were recognized using negative matrix factorization cluster analysis based on 50 iterations and the “brunet” method using the MET-correlated (METcor) and CNV-correlated (CNVcor) genes. The R package “iCluster” method was utilized to comprehensively analyze the EXP, MET, and DNA CNV profiles.Results: A total of 313 STAD samples were isolated for checking DNA copy numbers and MET and for measuring EXP. In accordance with our results, we discovered obvious co-regulation of CNVcor genes and METcor counterparts. Apart from that, these genes were subject to multi-omics integration. Meanwhile, three subtypes of STAD were detected and confirmed based on independent data. Among them, the subtype with increased aggressiveness was related to decreased mutation frequencies of ARID1A, PIK3CA, ZFHX3, SPECC1, OBSCN, KMT2D, FSIP2, ZBTB20, TTN, and RANBP2, together with the abnormal levels of JPH3, KCNB1, and PLCXD3.Conclusion: According to the results, these aforementioned genes exerted crucial roles in the development of invasive STAD. Our findings on transcriptomic regulation genomically and epigenetically facilitate the understanding of the STAD pathology from different aspects, which help to develop efficient anti-STAD therapy.
Exploring the Common Mechanism of Fungal sRNA Transboundary Regulation of Plants Based on Ensemble Learning Methods
Authors: Chi Junxia , Zhang Hao , Zhang Tianyue , Zhao Enshuang , Zhao Tianheng , Zhao Hengyi , Yuan Shuai 
Published: February 2022

Studies have found that pathogenic fungi and plants have sRNA transboundary regulation mechanisms. However, no researchers have used computer methods to carry out comprehensive studies on whether there is a more remarkable similarity in the transboundary regulation of plants by pathogenic fungi. In this direction, high-throughput non-coding sRNA data of three types of fungi and fungi-infected plants for 72 h were obtained. These include the Magnaporthe, Magnaporthe oryzae infecting Oryza sativa, Botrytis cinerea, Botrytis cinerea infecting Solanum lycopersicum, Phytophthora infestans and Phytophthora infestans infecting Solanum tuberosum. Research on these data to explore the commonness of fungal sRNA transboundary regulation of plants. First, using the big data statistical analysis method, the sRNA whose expression level increased significantly after infection was found as the key sRNA for pathogenicity, including 355 species of Magnaporthe oryzae, 399 species of Botrytis cinerea, and 426 species of Phytophthora infestans. Secondly, the target prediction was performed on the key sRNAs of the above three fungi, and 96, 197, and 112 core nodes were screened out, respectively. After functional enrichment analysis, multiple GO and KEGG_Pathway were obtained. It is found that there are multiple identical GO and KEGG_Pathway that can participate in plant gene expression regulation, metabolism, and other life processes, thereby affecting plant growth, development, reproduction, and response to the external environment. Finally, the characteristics of key pathogenic sRNAs and some non-pathogenic sRNAs are mined and extracted. Five Ensemble learning algorithms of Gradient Boosting Decision Tree, Random Forest, Adaboost, XGBoost, and Light Gradient Boosting Machine are used to construct a binary classification prediction model on the data set. The five indicators of accuracy, recall, precision, F1 score, and AUC were used to compare and analyze the models with the best parameters obtained by training, and it was found that each model performed well. Among them, XGBoost performed very well in the five models, and the AUC of the validation set was 0.86, 0.93, and 0.90. Therefore, this model has a reference value for predicting other fungi’s key sRNAs that transboundary regulation of plants.
Placental Macrophage (Hofbauer Cell) Responses to Infection During Pregnancy: A Systematic Scoping Review
Authors: Fakonti Georgia , Pantazi Paschalia , Bokun Vladimir , Holder Beth 
Published: February 2022

BackgroundCongenital infection of the fetus via trans-placental passage of pathogens can result in severe morbidity and mortality. Even without transmission to the fetus, infection of the placenta itself is associated with pregnancy complications including pregnancy loss and preterm birth. Placental macrophages, also termed Hofbauer cells (HBCs), are fetal-origin macrophages residing in the placenta that are likely involved in responding to placental infection and protection of the developing fetus. As HBCs are the only immune cell present in the villous placenta, they represent one of the final opportunities for control of infection and prevention of passage to the developing fetus.Objective and RationaleThe objective of this review was to provide a systematic overview of the literature regarding HBC responses during infection in pregnancy, including responses to viral, bacterial, and parasitic pathogens.MethodsPubMed and Scopus were searched on May 20th, 2021, with no limit on publication date, to identify all papers that have studied placental macrophages/Hofbauer cells in the context of infection. The following search strategy was utilized: (hofbauer* OR “hofbauer cells” OR “hofbauer cell” OR “placental macrophage” OR “placental macrophages”) AND [infect* OR virus OR viral OR bacteri* OR parasite* OR pathogen* OR LPS OR “poly(i:c)” OR toxoplasm* OR microb* OR HIV)].Outcomes86 studies were identified for review. This included those that investigated HBCs in placentas from pregnancies complicated by maternal infection and in vitro studies investigating HBC responses to pathogens or Pathogen-Associated Molecular Patterns (PAMPs). HBCs can be infected by a variety of pathogens, and HBC hyperplasia was a common observation. HBCs respond to pathogen infection and PAMPs by altering their transcriptional, translational and secretion profiles. Co-culture investigations demonstrate that they can replicate and transmit pathogens to other cells. In other cases, they may eliminate the pathogen through a variety of mechanisms including phagocytosis, cytokine-mediated pathogen elimination, release of macrophage extracellular traps and HBC-antibody-mediated neutralization. HBC responses differ across gestation and may be influenced by pre-existing immunity. Clinical information, including gestational age at infection, gestational age of the samples, mode of sample collection and pregnancy outcome were missing for the majority of studies.