bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diabetes impairs endothelial cell metabolic reprogramming and angiogenic responses to hypoxia, which are rescued by reconstituted high-density lipoproteins
Authors: Primer Khalia R. , Tan Joanne T.M. , Psaltis Peter J. , Bursill Christina 
Published: June 2022

Abstract
Aims
Diabetes-impaired angiogenic responses to ischemia contribute to vascular complications such as chronic wounds and a poorer prognosis post-myocardial infarction. Diabetes disrupts mechanisms involved in endothelial cell (EC) adaptation to hypoxia, which is essential for angiogenesis. The pyruvate dehydrogenase kinase 4 (PDK4)/pyruvate dehydrogenase complex (PDC) axis is an oxygen-conserving mechanism that preserves EC function in hypoxia, but its role in diabetes-impaired angiogenesis is unknown. We hypothesised that regulation of the PDK4/PDC axis is impaired by diabetes, important in angiogenesis and rescued by pro-angiogenic reconstituted high-density lipoproteins (rHDL).
Methods
In diabetic mice, PBS and rHDL were topically applied daily to full-thickness wounds. Wound PDK4 and phosphorylated PDC (pPDC) were measured by qPCR, western blotting and ELISA. Laser Doppler imaging measured wound blood flow reperfusion and immunohistochemistry assessed CD31+ neovessels. In vitro, human coronary artery ECs were preincubated with rHDL, exposed to high glucose (25Mm) and hypoxia (1.2% O2). Matrigel tubulogenesis and Boyden chambers assessed angiogenic functions, and the Seahorse Bioanalyser assessed cellular respiration. PDK4 was knocked down by siRNA, and chromatin immunoprecipitation determined transcription factor binding to the PDK4 promoter.
Results
Diabetes impaired the early induction of wound PDK4 and pPDC post-wound ischemia. Topical rHDL rescued this, enhancing PDK4 (68%, P < 0.05) and pPDC (165%, P < 0.01) levels. This was associated with increased wound neovascularization (62%, P < 0.05) and closure (154%, P < 0.0001). In vitro, PDK4 and pPDC levels were markedly increased in ECs exposed to hypoxia (65%, 70% respectively, P < 0.05). High glucose prevented further step-wise inductions, and caused an aberrant increase in mitochondrial respiration (19%, P < 0.05). Importantly, rHDL increased PDK4 mRNA levels and pPDC two-fold, returning mitochondrial respiration and EC angiogenic functions to normal glucose levels. PDK4 siRNA knockdown attenuated the proangiogenic effects of rHDL. rHDL appears to mediate these effects by increasing the activity of forkhead box O1 (FOXO1). We also show FOXO1 binds to the PDK4 promoter, which is enhanced by rHDL.
Conclusion
The PDK4/PDC axis response to hypoxia is important for angiogenesis and impaired by diabetes, which can be corrected by rHDL. These findings identify a new mechanism for the proangiogenic effects of rHDL in diabetes and have implications for its development as an agent that accelerates tissue regeneration.
The Impact of Early Adversity and Educational Attainment on Genetic and Brain Morphological Predictors of Intelligence
Authors: Donohoe Gary , Corley Emma , Fahey Laura , Fitzgerald Joan , Holleran Laurena , Walton Esther , Morris Derek 
Published: June 2022

Abstract
Intelligence is a strong predictor of occupational achievement, quality of life and physical health. While variation in intelligence is strongly heritable and has been robustly associated with early environment and brain morphology, little is known about how these factors combine and interact to explain this variation in intelligence. To address this, we modelled the relationship between common genetic variation, grey matter volume, early life adversity and education and intelligence in a UK Biobank sample of 16,383 individuals using structural equation modelling. We tested the hypotheses that total grey matter volume would mediate the association between genetics and intelligence, and that early life adversity and educational attainment would moderate this relationship. Common genetic variation was estimated using a genome-wide polygenic score (PGS) of intelligence. Consistent with previous studies, we found that grey matter volume was a significant mediator of the relationship between PGS and intelligence, but only when early life adversity was included in the model. We further found that higher educational attainment in turn moderated the effects of adversity. These findings suggest an important interaction between genetic and environmental factors in the development of intellectual functioning.
Assessment of Knowledge, Attitude and Practices on birth preparedness and complication readiness among pregnant women attending antenatal follow up at Mizan-Tepi University Teaching Hospital, Southwest Ethiopia
Authors: Mekonnen Olantu , Alelign Zewditu , Adugnaw Emebet , Neges Ketemaw 
Published: June 2022

Abstract
Background: Birth preparedness and complication readiness is a strategy to promote the timely used of skilled maternal and neonatal care with a skilled birth attendant, place of delivery and preparation in case of emergency during childbirth. Birth preparedness and complication readiness is the process of preparing for normal birth and anticipating the actions needed in case of an emergency. About 300 million women in developing world affected by short-term or long-term illness, even death by pregnancy and childbirth. This is because insufficiency birth and emergency preparedness.Objective: To assess the knowledge, attitude and practice on birth preparedness and complication readiness among women attending the ANC service at Mizan-Taipei university teaching hospital in Mizan Aman town October 1-29, 2021.Methods: An institutional based cross-sectional study was conducted among pregnant women attending ANC at Mizan-Tepi university teaching hospital in Mizan Aman town from October 1-29,2021. The systematic random sampling technique was used and a total of 157 women was interviewed using both close ended and open ended questions. The data collected was analyzed by SPSS version 23 and was presented using tables, bar graphs and pie chart.Result: Data were obtained from 157 pregnant women yielding, a response rate of 100%. Taking into account that 113 (72%) were knowledgeable about danger sign during pregnancy, 110 (70.1%) during labor and 80 (51%) in the postpartum period, 50 (34.4%) of them were considered as had good practice and the 125 (79.6%) respondent has a favorable attitude towards BP& CR.Conclusion and recommendation: The study identified good comprehensive knowledge and attitude in preparation for birth and its complication in the study area. Community education about preparation for birth and its complication and empowerment of women through the expansion of educational opportunities are recommended for improving birth preparedness and consequently the effects of pregnancy related complications.
Effect of light irradiation on sugar, phenolics and GABA metabolism on postharvest grape (Vitis vinifera L.) during storage
Authors: Nassarawa Sanusi Shamsudeen , Luo Zisheng 
Published: June 2022

Abstract
The impact of red and blue lightemitting diodes (LEDs) irradiation on the sugar metabolism and γaminobutyric acid in postharvest table grapes stored at (4 ℃) for 21 d after harvest were explored. Grapes fruit were exposed to red and blue light irradiation at the same intensity (500 LUX). The findings revealed that red and blue light treatments enhanced TSS and TA content. Furthermore, redlight irradiation retained higher phenolics compound during storage than bluelight and control. Redlight radiation sustained higher levels of phenolics biosynthesis related enzymes, including PAL, TAL, C4H, and 4CL than bluelight irradiation and control. The results showed that red-light was extremely effective at increasing glucose, fructose, and sucrose levels, which were 32.19 g kg-1, 33. 18 g kg-1 and 2.95 g kg-1 than those in blue-light and control at the end of storage and enhancing the SS-synthesis and acid invertase, while blue light enhanced SS-cleavage. In addition, red-light irradiation increased GAD activity while red and blue light irradiation inhibited GABA-T activity by 22.14 U kg-1 and 20.71 U kg-1 at 21 d of storage, compared to control, leading to GABA accumulation. These findings suggest that red and blue-light will help in maintaining the quality of grape during postharvest storage.
Evaluation of clinical practice of extraperitoneal cesarean section
Authors: Ji Chao , Chen Meng , Qin Yichen 
Published: June 2022

Abstract
Objective: To evaluate the extraperitoneal cesarean section in current as routine elective operation.Methods: 461 primiparas were divided into extraperitoneal cesarean section group and transperitoneal cesarean section group according to the operation type randomly, but in a non-blinded manner. The outcome measures were amount of intraoperative blood loss, operation duration, postoperative gas passage time, postoperative pain, postoperative complication and neonatal outcome. Results: The operation duration of the extraperitoneal cesarean section group were much lower than transperitoneal cesarean section group (P<0.05). The birth weight of the extraperitoneal cesarean section group was higher, and the number of neonatal transfers was less (P<0.05). There were no differences in other maternal surgical or neonatal complications between groups.Conclusion: The extraperitoneal cesarean section can be safely and valuably performed as a routine procedure in the surgical delivery of primiparas, but must be performed by well-trained surgeons.
Positive predictive value estimates for noninvasive prenatal testing from data of a prenatal diagnosis laboratory and literature review
Authors: Liu Siping , Yang Fang , Chang Qingxian , Jia Bei , Xu Yushuang , Wu Ruifeng , Li Liyan , Chen Weishan , Yin Ailan , Huang Fodi , Feng Suxin , Li Fenxia 
Published: June 2022

Abstract
Objective: Since 2011, noninvasive prenatal testing (NIPT) has undergone rapid expansion with both utilization and coverage. However, conclusive data regarding the clinical validity and utility of this testing tool, have lagged behind. Thus, there is a continued need to educate clinicians and patients about the current benefits and limitations to inform pre- and posttest counseling, pre/perinatal decision making, and medical risk assessment/management.Methods: A retrospective study among women referred for invasive prenatal diagnosis to confirm positive NIPT results between January 2017 and December 2020. Prenatal diagnosis testing were taken including karyotype, chromosomal microarray analysis (CMA) or CNV-seq. Positive predictive values (PPV) were calculated.Results: A total of 468 women were recruited. The PPVs for trisomy 21, 18, 13 were 86.1%, 57.8%, 25.0%, respectively. The PPV for RCAs and CNVs were 17.0%, 40.4% respectively. Detection of SCAs had PPV of 20% for monosomy X, 23.5% for 47,XXX, 68.8% for 47,XXY and 62.5% for 47,XYY. High-risk groups had a significant increase in the number of true positive cases compared to the low and moderate risk groups.Conclusion: T13, Monosomy X, RCA are associated with lower PPVs. Improvement of the cell-free fetal DNA screening technology and continued monitoring of its performance is important.
Basic influent sewage quality reflects sewershed characteristics in Tokyo city
Authors: Guo Zhongyu , Hatakeyama Takayuki , Yoshimura Chihiro , Wang Tingting , Hatano Yuta 
Published: June 2022

Abstract
Sewage comprises multifarious information on sewershed characteristics. For instance, influent sewage quality parameters (ISQPs) (e.g., total nitrogen (TN)) are being monitored regularly at all treatment plants. However, the relationship between ISQPs and sewershed characteristics is rarely investigated. Therefore, this study statistically investigated relationships between ISQPs and sewershed characteristics, covering demographic, social, and economic properties in Tokyo city as an example of a megacity. To this end, we collected ISQPs and sewershed characteristic data from 2015 to 2020 in 10 sewersheds in Tokyo city. By principal component analysis, spatial variability of ISQPs was aggregated into two principal components (89.8% contribution in total), indicating organics/nutrients and inorganic salts, respectively. Concentrations of organics/nutrients were significantly correlated with the population in sewersheds (daytime population density, family size, age distribution, etc.). Inorganic salts are significantly correlated with land cover ratios. Finally, a multiple regression model was developed for estimating the concentration of TN based on sewershed characteristics (R2=0.97). Scenario analysis using the regression model revealed that possible population movements in response to the coronavirus pandemic would substantially reduce the concentration of TN. These results indicate close relationships between ISQPs and sewershed characteristics and the potential applicability of big data of ISQPs to estimate sewershed characteristics and vice versa.
Determinants of bather hygiene in public swimming pools: a mixed-methods analysis of online discussion comments
Authors: Young Ian , Robillard Paige , Loeffler Pauline , Sekercioglu Fatih 
Published: June 2022

Abstract
Swimming in public pools can expose bathers to microbiological (e.g., Cryptosporidium) and chemical (e.g., disinfection by-product) hazards, which can lead to recreational water illness. Adequate hygienic behaviours among bathers are important to reduce these risks. However, prior studies have found low compliance with pre-swim showering and avoidance of urinating in pools. We conducted a mixed-methods analysis of online discussion comments to identify key determinants of these two behaviours. We identified relevant discussion threads on Reddit, Quora, and swimming forums. Identified comments were classified as having a positive or negative sentiment towards the behaviour, and they were thematically analyzed according to the Theoretical Domains Framework (TDF). We analyzed 986 comments from 45 threads; 49.8% of comments were classified as expressing a positive sentiment towards the behaviour. Positive sentiments were more commonly expressed towards pre-swim showering compared to avoidance of urinating in pools (60.1 vs. 47.1%). Eleven themes were identified across the following eight TDF domains: beliefs about consequences (n = 362 comments), social influences (n = 298), beliefs about capabilities (n = 240), social/professional role and identity (n = 144), knowledge (n = 138), emotions (n = 137), reinforcement (n = 78), and environmental context and resources (n = 33). Results can help to inform targeted educational and outreach strategies with bathers to encourage increased adoption of hygienic behaviours.
Carbapenemase-producing Aeromonas species isolated from the urban-impacted Akaki river in Ethiopia
Authors: Yitayew Berhanu , Woldeamanuel Yimtubezinash , Asrat Daniel , Aseffa Abraham , Olsson Per-Erik , Mihret Adane , Jass Jana 
Published: June 2022

Abstract
Carbapenemase-producing Aeromonas species are an emerging health threat. This study aimed to determine carbapenemase-mediated resistance among Aeromonas isolates from the Akaki river, Ethiopia during the dry and wet seasons in 2019–2020. Antimicrobial susceptibility to carbapenems and cephalosporins was determined and carbapenemase production was confirmed. Of 163 isolates, the majority were human pathogens Aeromonas caviae (62), Aeromonas hydrophila (33) and Aeromonas veronii (49). These isolates were resistant to carbapenem and cephalosporin antibiotics, with the highest resistance to cefotaxime 86 (59.7%), ertapenem 71 (49.3%) and imipenem 65 (45.1%). Resistance to carbapenem antibiotics varied between species, where most A. veronii 37 (75.5%) and A. hydrophila 28 (84.8%) were resistant to imipenem and all A.caviae were sensitive. A. veronii, A. caviae and A. hydrophila resistance to meropenem was 31 (63.3%), 3 (4.8%) and 19 (57.6%), respectively. Of isolates resistant to carbapenem, 82.1% A. hydrophila and 94.4% A. veronii were carbapenemase producers. Cephalosporin resistance also varied among the different species. The highest resistance to carbapenem antibiotics was in isolates collected during the wet season (p&lt;0.05); however, it was not consistent across all classes of antibiotics tested. The rivers in megacities could be reservoirs of carbapenemase-producing Aeromonas spp.
Risk assessment of non-point source pollution in karst reservoirs based on ‘source–sink’ landscape theory
Authors: Zhao Weiquan , Zhou Zhongfa , Zhao Zulun , Li Wei , Li Qiuhua 
Published: June 2022

Abstract
The quantitative identification of areas at risk for such pollution is conducive to allocating limited government funds to critical areas and the efficient and economical management of water environments. Here, the Baihua Lake watershed, an important drinking water source for Guiyang City, was taken as the study area. The location-weighted landscape contrast index (LCI) and non-point source pollution risk index (NSPRI) were developed based on the ‘source–sink’ landscape theory. The method takes into account the risk of pollution source formation and pollutant transport. A total of 348 natural sub-watersheds were used as assessment units by estimating the nitrogen and phosphorus pollution emission (absorption) potentials of different landscape types in the sub-watersheds and considering the influence of vegetation cover, distance from the reservoir, and slope in the transmission process, a quantitative assessment of Baihua Lake's pollution was carried out; the reliability of the method was verified by comparing the assessment results with measured water quality data and field surveys. The results indicate (1) 132 sub-watersheds (37.93%) dominated by source effects, mainly distributed in Yanshanhong Township, Yeya Township, and the Qinglong Subdistrict, with construction land and farmland as the main landscape types, and 216 sub-watersheds (62.07%) dominated by sink effects, mainly distributed in Zhanjie and Baihuahu Townships, with forests as the primary landscape type. (2) Additionally, 17 sub-watersheds (4.89%) show extremely high risk for non-point source pollution; these watersheds are mainly distributed in the Qinglong Subdistrict and mainly consist of urban residential areas and schools. These sub-watersheds discharge a large volume of sewage, which threatens the water quality of the upper reaches of Baihua Lake and must be managed. (3) The rivers corresponding to relatively high-risk, high-risk, and extremely high-risk sub-watersheds include the Dongmenqiao, Limu, Changchong, and Maixi Rivers.
The water audit mode of water environment and water ecology based on evaporation in industrial parks
Authors: Zhang X. L. , He W. T. , Li L. Y. , Wang Y. R. , Li X. J. , Li X. , Li H. Y. 
Published: June 2022

Abstract
With large-scale industrial parks developing, concern regarding high evaporation and its spillover impacts on water environment and water ecology has grown significantly in recent years. However, it is hard for the traditional water audit to meet the needs of the collaborative management of water environment and water ecology based on evaporation (3E), which requires a multi-dimensional evaluation. The purpose of this paper is to accurately evaluate industrial parks' impact considering the water life-cycle. This paper proposes a systematic 3E water audit method that contains a logical framework and a comprehensive evaluation index system with five dimensions and 25 indexes, then applies it to a typical iron and steel industrial park in China. The results presented the 3E audit score of the Chengsteel IP as 89.91, suggesting comprehensive performance is good. The score and grade of every index, especially the quantity of water evaporation per ton of steel, were provided. This paper indicates that the new method developed can systematically evaluate the impact of industrial parks. In addition, our analysis highlights the different management strategies and practices that relieve the impacts of the whole water-use process on legal, eco-environmental, economic, social, and technical dimensions.
Enhancement of E-Peroxone process with waste-tire carbon composite cathode for tinidazole degradation
Authors: Xu Anlin , Liu Wanqun , Chu Leping , Zhang Yunhai , He Yide , Zhang Yongjun 
Published: June 2022

Abstract
The cathode is the key component in the electro-peroxone process (E-Peroxone), which is popularly constructed with carbon materials. This study developed an innovative method to fabricate a cathode with waste-tire carbon (WTC) whose performance was evaluated for the degradation of tinidazole (TNZ), an antibiotic frequently detected in water. It was found that the addition of WTC in the cathode can significantly promote the yield of H2O2 and the current efficiency: around 2.7 times that of commercial carbon black at the same loading. The critical influencing factors were studied, including the current density, ozone concentration, initial pH value, chlorine ions and initial TNZ concentration. The scavenger tests demonstrated the possible involvement of •OH and . Some transformation products of TNZ were identified with UPLC-MS and the degradation pathway was proposed accordingly. These results demonstrated the potential of WTC for developing E-Peroxone cathodes.
Long Non-coding RNAs: Pivotal Epigenetic Regulators in Diabetic Retinopathy
Authors: Chen Peng , Song Zhaoxia , He Chang , Wen Jianping , Yang Jianli 
Published: June 2022

Abstract:
Diabetic retinopathy (DR) is a severe complication of diabetes; however, its mechanism is
not fully understood. Evidence has recently revealed that long non-coding RNAs (lncRNAs) are abnormally
expressed in DR, and lncRNAs may function as pivotal regulators. LncRNAs are able to
modulate gene expression at the epigenetic level by acting as scaffolds of histone modification complexes
and sponges of binding with microRNAs (miRNAs). LncRNAs are believed to be important
epigenetic regulators, which may become beneficial in the diagnosis and therapy of DR. However, the
mechanisms of lncRNAs in DR are still unclear. In this review, we summarize the possible functions
and mechanisms of lncRNAs in epigenetic regulation to target genes in the progression of DR.
Targeting Glioma Cells with Nutraceuticals: Therapeutic Effects Based on
Molecular Mechanisms, New Evidence and Perspectives
Authors: Salami Marziyeh , Salami Raziyeh , Aarabi Mohammad-Hossein , Mafi Alireza , Ghorbanhosseini Seyedeh Sara , Shafabakhsh Rana , Asemi Zatollah 
Published: June 2022

Abstract:
Gliomas are the most common malignant cancers of the brain that have unregulated proliferation
and are known as highly invasive tumors. Hence, their relapse rate is high, and the prognosis is
low. Despite remarkable advances in neuroimaging, neurosurgery, and radiation therapy, they, especially
glioblastoma, are highly resistant to treatments, including radiotherapy, surgery, and temozolomide
chemotherapy. The average survival rate for patients with malignant glioma is still less
than two years. Accordingly, the search for new treatment options has recently become an urgent
need. Today, a number of nutraceuticals have been considered because of their special role in inhibiting
the angiogenic process, metastasis, and apoptosis, resulting in the inhibition of tumor growth, including
glioma. Nutraceuticals can disrupt cancer cells by affecting different pathways. In fact, these
compounds can reduce the growth of cancer cells, inhibit their proliferation and angiogenesis, as well
as induce apoptosis in these cells and play an important role in various stages of treatment. One of the
key targets of nutraceuticals may be to regulate cellular signaling pathways, such as
PI3K/Akt/mTORC1, JAK/STAT, and GSK-3, or to exert their effects through other mechanisms, such
as cytokine receptors and inflammatory pathways, reactive oxygen species, and miRNAs. This review
refers to the results of recent studies and target molecules as well as signaling pathways affected by
some nutraceuticals in glioma cells. These studies indicated that clinical trials are imminent and new
approaches can be beneficial for patients.