bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomics of the Skin Secretion of Pulchrana glandulosa (Anura:
Ranidae) (Boulenger, 1882), Langkawi Island, Kedah, Peninsular Malaysia
Authors: Shahrudin Shahriza , Ong Dasi , Ismail Mohd Nazri 
Published: December 2022

Background:
The advancement of proteomics studies leads to various benefits in research.
However, in Malaysia, proteomics studies are still in their early stage.

Objective:
Four adult Pulchrana glandulosa individuals were collected from the stream of Temurun
Waterfall for their proteomics information.

Methods:
These frogs’ skin secretions were collected, extracted, and analysed for their protein antimicrobial
peptide compounds and biomedical potentials using liquid chromatography-mass spectrometry.

Results:
Forty-six proteins had been identified from the skin secretion of this species. They contained
unreviewed proteins, enzymes, AMPs, receptors, regulatory, transport, hormone, and developmental
proteins. In addition, 11 AMP had been identified, consisting of esculentin-2, brevinin 1, and other
AMPs.

Conclusion:
These proteins and AMPs have a wide range of biomedical importance, such as antimicrobials,
anti-tumour, anti-cancerous, anti-viral, wound healing, anti-inflammation, anti-ageing and
maintaining homeostasis.
Gene Expression Network and Circ_0008012 Promote Progression in
MLL/AF4 Positive Acute Lymphoblastic Leukemia
Authors: Tang Yan-Lai , Su Jia-Yin , Luo Jie-Si , Zhang Li-Dan , Zheng Li-Min , Liang Cong , Wang Li-Na , Li Yu , Fan Zhong , Huang Dan-Ping , Sun Panpan , Luo Zhenhua , Qi Ning Hao , Lan Jing-Jing , Zhang Xiao-Li , Huang Li-Bin , Luo Xue-Qun 
Published: December 2022

Background:
Acute lymphoblastic leukemia with MLL/AF4 rearrangement remains a
major hurdle to improving outcomes. Gene network and circRNAs have been found to participate
in tumorigenesis, while their roles in leukemia still need to be explored. Recent patents have
shown that circRNAs exhibit the markers for the children ALL, although the target and related
mechanism remain to be elucidated.

Objective:
This study aims to explore the possible targets and mechanisms of ALL with MLLAF4
rearrangement.

Methods:
We first generated a gene network focusing on MLL-AF4 rearrangement. Cell viability
was determined with Cell Counting Kit-8 assay. The cell apoptosis was tested by the Annexin
V/PI assay. The RNA-protein complexes were analyzed by qRT-PCR, and the pathway proteins
were analyzed by western blot.

Results:
This gene network was associated with biological processes, such as nucleic acid metabolism
and immunity, indicating its key role in inflammation. We found that circ_0008012 was upregulated
in MLL/AF4 ALL cells and regulated cell proliferation and apoptosis. Further computed
simulation and RIP showed that IKKβ was the strongest protein in the NF-κB pathway binding
with circ_0008012. As a result, possible regulation of circ_0008012 is suggested by binding IKKβ
in the IKKα:IKKβ:IKKγ compound, which then phosphorylates IκB and activates NF-
κB:p65:p300 compound in cell nucleus, thereby leading to leukemia.

Conclusion:
We identified a gene network for MLL/AF4 ALL. Moreover, circ_0008012 may be a
therapeutic target for this subtype of ALL.
Arnicolide D Inhibits Oxidative Stress-induced Breast Cancer Cell Growth and
Invasion through Apoptosis, Ferroptosis, and Parthanatos
Authors: Wen Wei , Jin Ke , Che Ying , Du Lin-Yao , Wang Li-Na 
Published: December 2022

Background:
Breast cancer is the most common malignant tumor in women, and its pathogenesis is very
complicated. More and more studies have found that Traditional Chinese Medicine plays an important role in tumor
prevention.

Objective:
To investigate the mechanism of arnicolide D isolated from Centipeda minima in breast cancer.

Methods:
Cell Counting Kit-8 (CCK-8), western blot, RT-qPCR, ELISA, flow cytometry, and Transwell were used to
detect the effect of arnicolide D on the biological function of breast cancer cells.

Results:
Arnicolide D promoted reactive oxygen species (ROS) production and induced a decrease in mitochondrial
membrane potential in breast cancer cells, thereby inhibiting cell viability and increasing lactate dehydrogenase (LDH)
release. Arnicolide D activated the classical apoptosis pathway to induce cell apoptosis; it significantly promoted
PARP-1 expression, enhanced the nuclear translocation of apoptosis-inducing factor (AIF), and reduced the expression
of AIF in mitochondria, indicating that it can induce the occurrence of parthanatos in a ROS dependent manner. In
addition, arnicolide D down-regulated glutathione peroxidase 4 (GPX4) expression and increased the accumulation of
Fe2+ and malondialdehyde (MDA), thereby activating ferroptosis. Apoptosis inhibitor, ferroptosis inhibitor, PARP
inhibitor, PARP-1 siRNA, AIF siRNA and GPX4 overexpression vector significantly attenuated the inhibitory effect of
arnicolide D on cell viability and reduced LDH release, which indicates that arnicolide D inhibits breast cancer cell
growth by inducing apoptosis, parthanatos and ferroptosis. Arnicolide D also reduced breast cancer cell invasion and
inhibited the expression of matrix metallopeptidase (MMP)-2 and MMP-9.

Conclusion:
Arnicolide D can activate a variety of cell death modes by inducing oxidative stress, thereby inhibiting
the growth and invasion of breast cancer cells, indicating that arnicolide D has a good anti-tumor effect.
Assessment of Learning Approaches of Saudi Dental Students Using the Revised Two-Factor Study Process Questionnaire: A Cross-Sectional Study
Authors: Alahmari Fatemah , Basudan Amani , Shaheen Marwa 
Published: December 2022

Purpose:
The aim of this study was to assess the learning approaches of undergraduate dental students in Saudi Arabia.

Methods:
This was a cross-sectional study in which an electronic questionnaire using the Biggs Revised two-factor Study Process Questionnaire (R-SPQ-2F) was completed by 222 undergraduate dental students from 1st year to 5th year. R-SPQ-2F contains 20 items to measure learning approaches through a structural model contrasting deep and surface learning.

Results:
The mean value of the deep approach was higher among 4th- and 5th-year students than among 1st-, 2nd-, and 3rd-year students. The mean values of the surface approach in male subjects and subjects with lower grade point averages were statistically significantly higher than those of the deep approach.

Conclusion:
This study highlights that dental students have a greater tendency to adopt the surface approach in their preclinical years and in the 3rd year when they experience a transition to clinical training. A deep approach to learning was mostly adopted among 4th- and 5th-year dental students. The surface approach was higher among male than female students. Students who used a deep approach had higher academic achievement than students who used a surface approach.
Portland Cement: An Overview as a Root Repair Material: Applications and Various Modifications
Authors: Shahi Shahriar , Fakhri Elaheh , Dizaj Solmaz Maleki , Salatin Sara , Sharifi Simin , Rahimi Saeed 
Published: December 2022

Background:
Portland cement has promisingly been utilized for the reconstruction of root perforation and bone defects, although its key drawbacks, including low mechanical properties and radiopacity as well as long setting time, necessitate pragmatic modifications.

Objective:
The main objective of this review was an overview of portland cement as a root repair material, its applications and various modifications.

Methods:
The electronic search of the literature was done on the Pubmed and Google Scholar databases with the keywords of Portland cement, carbon nanotube, graphene oxide, MTA, pulp capping, and root repair material.

Results:
The first part of this paper presents the data published in the literature on applications of Portland cement in endodontic situations, including vital pulp therapy, root perforation repair, root canal filling and root-end filling following apical endodontic surgery. This bioactive endodontic cement has shown promising success rates compared to mineral trioxide aggregate (MTA), however, considerable modifications are required in order to improve its clinical performance and expand its application scope as a root repair material. Hence, nano-reinforcements (graphene oxide, carbon nanotube, silica and hydroxyapatite) and extensive chemical modifications incorporated into Portland cement composition to produce innovative bio-dental materials with superior rheological properties have been discussed. Moreover, the current knowledge of the microstructure, mechanical properties and durability of nanomaterial-incorporated cement has been summarized. Ultimately, this article outlines the main points of animal and clinical studies on resin-modified Portland cement (TheraCal) as a pulp capping material and suggests further investigations prior to marketing authorization.

Conclusion:
It can be concluded that Portland cement has the potential to be used as an acceptable pulp capping material with the least complaints in the long term.
Microwave-Assisted Flow Chemistry for Green Synthesis and Other
Applications
Authors: Török Béla , Mooney Tara , Ilamanova Maysa 
Published: December 2022

Abstract:
Using combined microwave-assisted flow chemistry approaches is one of the most active
areas of microwave chemistry and green synthesis. Microwave-assisted organic synthesis
(MAOS) has contributed significantly to developing green synthetic methods, while flow chemistry
applications are quite popular in industrial chemistry. The combination of the two has farreaching
advantages. In early studies, the flow chemistry concept was applied in domestic microwave
ovens already indicating strong potential for future applications. The relatively small diameter
of the flow reactors can address the limited penetration depth of microwaves, which is a major
impediment in large-scale batch reactors. With the commercial availability of dedicated microwave
synthesizers with tunable frequencies and better temperature control, the possibilities to apply
flow synthesis grew even broader. The developments focus on several issues; the two major
ones are the design and application of reactors and catalysts. Common reactor types include microwave-
absorbing, such as silicon carbide, and microwave-transparent materials, such as borosilicate
glass, quartz, or Teflon, with the catalyst or solvent adjusted accordingly. Several heterogeneous
catalysts are considered strong microwave absorbers that can heat the reaction from inside the
reactor. Such materials include clays, zeolites, or supported metal catalysts. Here, the major advances
in design and applications and the benefits gained will be illustrated by synthesizing fine
chemicals, from organic compounds to nanoparticles and new materials.
Overview of Nutritional Knowledge of Pregnant Women about the First 1000 Days of Life
Authors: Gultom Abitmer , Patriawati Keswari Aji 
Published: December 2022

The First Thousand Days of Life (1000 HPK) is a golden period starting from 0-2 years, which is a critical period in brain growth due to a rapid increase in the development of cells in the brain that are very susceptible to damage. If the child suffers from malnutrition at this time, it cannot be guaranteed that further development will run normally. This study aims to describe the level of nutritional knowledge of pregnant women about the first 1000 days of life at the Duren Sawit Health Center in 2019. This study is descriptive. Sampling using a non-random sampling technique with purposive sampling type and obtained a total sample of 100 respondents. The results showed that the description of the nutritional knowledge of pregnant women about the first 1000 days of life at the Duren Sawit Health Center in 2019 showed that most pregnant women had sufficient knowledge of 72 people (72.0%), good knowledge of 13 people (13.0% ), and lack of understanding as many as 15 people (15.0%). For further research, it takes a long time and better measuring tools to increase the accuracy of the research results.
Keywords: Knowledge of Nutrition, Pregnant Women, First 1000 Days of Life
Relationship of Occupational Safety and Health Knowledge to The Occupation of Paramedical Occupational Accidents
Authors: Prihantini Nur Nunu , Hutagalung Patar , Novelyn Silphia 
Published: December 2022

Occupational health and safety is a fundamental issue. Work accidents, directly or indirectly, can cause losses to the company, among others, late completion of work, decreased productivity, and healing costs for employees. This study aims to determine the relationship between knowledge of occupational safety and health behavior with the occurrence of paramedic work accidents at UKI Hospital in 2018. This type of research is correlational, with a total of 30 samples. This study found a relationship between knowledge of occupational safety and health with attitudes and actions to prevent work accidents among nurses at UKI Hospital.
Keywords: Occupational Accident, Occupational Health and Safety, Paramedic.
The Relationship Between Sitting Attitude and Duration of Work with Low Back Pain Complaints Among Kalimantan Tengah Health Office Employees in 2019
Authors: Hendrika Wendy , Sitompul Yunita R.M.B , Petrus Gunadi 
Published: December 2022

Low back pain is an occupational disease that arises due to work relationships or is caused by work and work attitudes. Incorrect sitting posture and sitting for long periods are one of the most frequent risk factors. The study was conducted to determine the relationship between sitting posture and work duration with complaints of low back pain in-office employees. The research design used is descriptive-analytic with a cross-sectional approach. The number of samples was 70 employees of the Central Kalimantan Provincial Health Office using the total sampling technique. The research data was taken using a questionnaire. The results of respondents who complained of low back pain were 12 people (17.1%), and 9 of them related to the wrong sitting position (81.8%) with a p-value of 0.000, and the wrong sitting posture was 84 times greater for suffering from lower back pain compared to the correct sitting posture. The duration of work (long sitting) did not have a relationship with complaints of back pain (p = 0.974). There is a significant relationship between the respondent's sitting attitude to the incidence of low back pain, and there is no significant relationship between work duration (length of sitting) and the incidence of low back pain in employees at the Central Kalimantan Provincial Health Office.
Keywords: Work Duration, Sitting Length, Lower Back Pain, Sitting Position
Transdermal Patch: A Novel Approach for Transdermal Drug Delivery
Authors: Tiwari Chanchal , Choudhary Mahima , Malik Princy , JAISWAL PANKAJ KUMAR , Chauhan Reetu 
Published: December 2022

A self-contained, covert, medicated adhesive patch known as a transdermal patch offers a practical mode of delivery for a range of skin and body problems. Multiple drug administration has several disadvantages including inconvenient administration, the risk of overdose, lack of patient compliance, and drug plasma level fluctuations. Transdermal medication delivery has emerged as a creative means of achieving systemic drug absorption at a predefined rate over an extended period. Its primary benefits are reduced dose frequency, avoiding first-pass metabolism by entering directly into the systemic circulation, suitability for elderly patients who cannot take pharmaceuticals orally, and ability to be self-administered with fewer adverse effects. This review covers general aspects like drug absorption pathways through the skin, the kinetics of drug absorption, different factors affecting the transdermal permeability, various types of transdermal patches, their components, and evaluation parameters. Additionally, some marketed transdermal patches and therapeutic applications of transdermal drug delivery systems have been discussed. Moreover, the article includes various generations of advancements in the transdermal drug delivery system and its future aspect.
Keywords- Transdermal patch, Permeability, Polymer Matrix, Rate Controlling Membrane, Permeation Enhancers.
Measurement of Plasma Fibrinogen and D-dimer Levels in Sudanese Hypertensive Patients at Khartoum state
Authors: Ebar Mohamed Hassan Osman , Ali Ahmed Gafer Ahmed , Adan Mohamed Qaje , Ahmed Albara , Isse Mohamed Osman Mohamed 
Published: December 2022

Background: Hypertension is a chronic elevation of blood pressure that, in the long-term, causes end organ damage and results in increased morbidity and mortality. Thrombosis often appears to complicate the course of patients with hypertension; thrombosis in some patients with hypertension could be developed to organ damage. The aim of this study was to measure fibrinogen and D-dimer level among sudanese patients with hypertension.
Materials and method: This was a case control study conducted at Khartoum state, Sudan during the period from May 2022 to August 2022 to measure fibrinogen and D-dimer levels in sudanese patients with hypertension. Blood samples were collected, 50 from the patients as case group, and 50 from normal healthy subjects as control group. A total of three ml of whole blood were collected from each participant in sodium citrated tube. The fibrinogen and D-dimer level measurement was done by using coagulometer (M1 coatron) and ichroma II device respectively.
Results: The results of this study revealed that when compared Fibrinogen level and D-dimer between cases and control group; the results revealed that fibrinogen level was significant increase in hypertensive patients with P-value of 0.000. Also, D-dimer was significant increase in hypertensive patients when compared with the results of control group, and showed significant association with P-value of 0.000. There was a negative correlation between hypertension and the gender, but the age showed positive correlation with hypertension and with D-dimer, where both fibrinogen and D-dimer showed positive correlation with the duration of hypertension.
Conclusion: This study concluded; when compared the fibrinogen level and D- dimer between cases and control group, there was significant increase in fibrinogen and D- dimer.
Keywords: Hypertension, fibrinogen level, D-dimer, coronary artery disease, ischemic heart disease, Cardiovascular disease, DIC, fibrin degradation products
Efficacy and Safety of Intermittent Fasting in People With Insulin-Treated Type 2 Diabetes (INTERFAST-2)—A Randomized Controlled Trial
Authors: Obermayer Anna , Tripolt Norbert J. , Pferschy Peter N. , Kojzar Harald , Aziz Faisal , Müller Alexander , Schauer Markus , Oulhaj Abderrahim , Aberer Felix , Sourij Caren , Habisch Hansjörg , Madl Tobias , Pieber Thomas , Obermayer-Pietsch Barbara , Stadlbauer Vanessa , Sourij Harald 
Published: December 2022

OBJECTIVE
To investigate the safety and feasibility of 3 nonconsecutive days of intermittent fasting (IF) per week over 12 weeks in participants with insulin-treated type 2 diabetes.

RESEARCH DESIGN AND METHODS
Forty-six people were randomized to an IF or control group. Dietary counseling and continuous glucose monitoring was provided. Coprimary end points were the change in HbA1c from baseline to 12 weeks and a composite end point (weight reduction ≥2%, insulin dose reduction ≥10%, and HbA1c reduction ≥3 mmol/mol).

RESULTS
The IF group showed a significant HbA1c reduction (−7.3 ± 12.0 mmol/mol) compared with the control group (0.1 ± 6.1 mmol/mol) over 12 weeks (P = 0.012). The coprimary end point was achieved by 8 people in the IF and none in the control group (P &lt; 0.001). No severe hypoglycemia occurred.

CONCLUSIONS
IF is a safe and feasible dietary option to ameliorate glycemic control while reducing total daily insulin dose and body weight in insulin-treated people with type 2 diabetes.
High-fat diet-induced deSUMOylation of E4BP4 promotes lipid droplet biogenesis and liver steatosis in mice
Authors: Wang Sujuan , Yang Meichan , Li Pei , Sit Julian , Wong Audrey , Rodrigues Kyle , Lank Daniel , Zhang Deqiang , Zhang Kezhong , Yin Lei , Tong Xin 
Published: December 2022

<p>Dysregulated lipid droplet accumulation has been identified as one of the main contributors to liver steatosis during nonalcoholic fatty liver disease (NAFLD). However, the underlying molecular mechanisms for excessive lipid droplet formation in the liver remain largely unknown. In the current study, hepatic E4 promoter-binding protein 4 (E4BP4) plays a critical role in promoting lipid droplet formation and liver steatosis in a high-fat diet (HFD)-induced NAFLD mouse model. Hepatic <em>E4bp4</em> deficiency (<em>E4bp4-LKO</em>) protects mice from HFD-induced liver steatosis independently of obesity and insulin resistance. Our microarray study showed a markedly reduced expression of lipid droplet binding genes such as <em>Fsp27</em> in the liver of <em>E4bp4-LKO</em> mice. E4BP4 is both necessary and sufficient to activate <em>Fsp27</em> expression and lipid droplet formation in primary mouse hepatocytes. Overexpression of <em>Fsp27</em> increased lipid droplets and triglycerides in <em>E4bp4-LKO</em> primary mouse hepatocytes and restored hepatic steatosis in HFD-fed <em>E4bp4-LKO</em> mice. Mechanistically, E4BP4 enhances the transactivation of <em>Fsp27</em> by CREBH in hepatocytes. Furthermore, E4BP4 is modified by SUMOylation and HFD feeding induces deSUMOylation of hepatic E4BP4. SUMOylation of five lysine residues of E4BP4 is critical for the downregulation of <em>Fsp27 </em>and lipid droplets by the cAMP signaling in hepatocytes. Taken together, this study revealed that E4BP4 drives liver steatosis in HFD-fed mice through its regulation of lipid-droplet binding proteins. Our study also highlights the critical role of deSUMOylation of hepatic E4BP4 in promoting NAFLD. </p>
Pengembangan kawasan Linggai Park untuk mewujudkan program revitalisasi wisata Danau Maninjau
Authors: Syahril Syahril , Nabawi Rahmat Azis , Wulansari Rizky Ema , Alwifari M. Qari , Fitra Rizki , Preti Agia Kulkarni , Aditia Jefri , Aldio M. Fikhri 
Published: December 2022

Revitalisasi wisata Danau Maninjau perlu didukung agar dapat kembali menjadi destinasi wisatawan. Salah satu destinasi wisata yang dikembangkan oleh Pemerintahan Kabupaten Agam di Kawasan Danau Maninjau adalah Linggai Park. Oleh karena itu perlu sekali didukung dengan adanya kesadaran dan kepedulian dari masyarakat untuk dapat mengembangkan potensi wisata ini. Berdasarkan hasil obseravasi dan wawancara dengan beberapa orang wisatawan yang berkunjung ke Linggai park, perlu adanya wahana yang dapat menarik minat wisatawan untuk berkunjung. Berdasarkan hal tim Desa Wisata Edukatif dari Universitas Negeri Padang melaksakan kegiatan pengabdian yang dapat mengembangkan parawisata Linggai Park. Metode pelaksanaan kegiatan dilakukan dengan memberikan edukasi dan pelatihan pembuatan perahu wisata dari fiberglass. Kegiatan edukasi sadar dan peduli wisata diberikan kepada siswa SMP Negeri 2 Tanjung Raya. Kesadaran dan kepedulian dari generasi muda sangatlah penting, karena mereka dapat menjadi influencer dalam promosi wisata Linggai park. Kegiatan pelatihan pembuatan perahu wisata dari bahan fiberglass diberikan kepada pemuda Nagari Duo Koto. Perahu wisata dari bahan fiberglass dari hasil pelatihan dihibahkan kepada Nagari Duo Koto. Harapanya setelah pelatihan ini masyarakat dapat membuat perahu wisata secara mandiri.