bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bulbar projecting subcortical GABAergic neurons send collateral branches extensively and selectively to primary olfactory cortical regions
Authors: Hook Chelsea , Puche Adam C. 
Published: December 2022

AbstractCircuit operations of the olfactory bulb are modulated by higher order projections from multiple regions, many of which are themselves targets of bulbar output. Multiple glutamatergic regions project to the olfactory bulb, including the anterior olfactory nucleus (AON), prefrontal cortex (PFC), piriform cortex (PC), entorhinal cortex (EC), and tenia tecta (TT). In contrast, only one region provides GABAergic projections to the bulb. These GABA neurons are located in the horizontal limb of the diagonal band of Broca extending posteriorly through the magnocellular preoptic nucleus to the nucleus of the lateral olfactory bulb. However, it was unclear whether bulbar projecting GABAergic neurons collaterallize projecting to other brain regions. To address this, we mapped collateral projections from bulbar projecting GABAergic neurons using intersectional strategies of viral and traditional tract tracers. This approach revealed bulbar projecting GABAergic neurons show remarkable specificity targeting other primary olfactory cortical regions exhibiting abundant collateral projections into the accessory olfactory bulb, AON, PFC, PC, and TT. The only "nonolfactory" region receiving collateral projections was sparse connectivity to the medial prefrontal orbital cortex. This suggests that basal forebrain inhibitory feedback also modulates glutamatergic feedback areas that are themselves prominent bulbar projection regions. Thus, inhibitory feedback may be simultaneously modulating both synaptic processing of olfactory information in the bulb and associational processing of olfactory information from primary olfactory cortex. We hypothesize that these olfactory GABAergic feedback neurons are a regulator of the entire olfactory system.
Ultrawide Hydrazine Concentration Monitoring Sensor Comprising Ir−Ni Nanoparticles Decorated with Multi‐Walled Carbon Nanotubes in On‐Site Alkaline Fuel Cell Operation
Authors: Nde Dieudonne Tanue , Park Jihyeon , Lee Sang Hyuk , Lee Jaeyoung , Lee Hye Jin 
Published: December 2022

AbstractA highly sensitive amperometric hydrazine monitoring sensor offering an ultrawide dynamic range of 5 μM to 1 M in alkaline media (e. g., 1 M KOH) was developed via co‐electrodepositing iridium‐nickel alloy nanoparticles (NPs) functionalized with multi‐walled carbon nanotubes (Ir−Ni−MWCNTs) on a disposable screen‐printed carbon electrode. The synergistic interaction of MWCNTs with Ir−Ni alloy NPs resulted in enlarged active surface area, rapid electron transfer, and alkaline media stability with an onset potential of −0.12 V (vs. Ag/AgCl) toward hydrazine oxidation. A limit of detection for hydrazine was 0.81 μM with guaranteed reproducibility, repeatability, and storage stability alongside a superb selectivity toward ethanolamine, urea, dopamine, NaBH4, NH4OH, NaNO2, and Na2CO3. The sensor was finally applied to on‐site monitoring of the carbon‐free hydrazine concentration at the anode and cathode of a hydrazine fuel cell, providing more insight into the hydrazine oxidation process during cell operation.
Pyrene‐Based D‐A Molecules as Efficient Heterogeneous Catalysts for Visible‐Light‐Induced Aerobic Organic Transformations
Authors: Jiang Zhihui , Sun Wenhao , Yang Zhenyan , Pan Hui , Tang Zubing , Shi Wei , Xiang Yonggang , Yan Dingce , Teng Huailong 
Published: December 2022

AbstractIn this work, an efficient visible light promoted aerobic dehydro‐coupling of amines, oxidation of thioethers and hydroxylation of arylboronic acids under benign conditions by using pyrene‐based donor‐acceptor (D‐A) conjugated organic molecules was described. Donor‐acceptor structure influences their π‐conjugation and band gap a lot, and thereby enhances their visible light absorption ability, single electron transfer and oxidative behaviors. Alkynyl units in PS‐IV play a crucial role in the catalyst which could serve as electron transferring bridge to strengthen electron delocalization, thus facilitating the single electron transfer from photosensitizer to substrates, and making it an efficient ⋅O2− generator. While PS‐III without alkynyl units tends to produce 1O2. Therefore, these molecules can serve as efficient catalysts for different kinds of visible‐light‐induced aerobic organic reactions. More importantly, the simply structured molecule is insoluble and stable in various solvents, and thus could be recycled as heterogeneous catalyst for many rounds with slight catalytic activity degradation. Besides, large scale (1 mol) reaction of benzylamine coupling proceeded smoothly under the standard conditions.
Detection and Semi‐Quantification of Lipids on High‐Performance Thin‐Layer Chromatography Plate Using Ceric Ammonium Molybdate Staining
Authors: Asressu Kesatebrhan Haile , Zhang Qibin 
Published: December 2022

AbstractIt is desirable to quickly check the composition of lipids in small size samples, but achieving this is challenging using the existing staining methods. Herein, a highly sensitive and semi‐quantitative method is developed for analysis of lipid samples with ceric ammonium molybdate (CAM) staining. The CAM detection method is systematically evaluated with a wide range of lipid classes including phospholipids, sphingolipids, glycerolipids, fatty acids (FA), and sterols, demonstrating high sensitivity, stability, and overall efficiency. Additionally, CAM staining provides a clean yellow background in high performance thin‐layer chromatography (HPTLC) which facilitates quantification of lipids using image processing software. Lipids can be stained with CAM reagent regardless of their head group types, position of the carbon–carbon double bonds, geometric isomerism, and the variation in the length of FA chain, but staining is mostly affected by the degree of unsaturation of the FA backbone. The mechanism of the CAM staining of lipids is proposed on principles of the reduction–oxidation reaction, in which Mo(VI) oxidizes the unsaturated lipids into carbonyl compounds on the HPTLC plate upon heating, while itself being reduced to Mo(IV). This method is applied for the separation, identification, and quantification of lipid extracts from porcine brain.Practical applications: The CAM staining method that is developed in this work shows a high sensitivity for diverse lipid classes following HPTLC separation. HPTLC with CAM staining is a promising method for quick assessment of the identity and quantity of diverse lipid classes in lipid extracts of small size biological samples.
Ruthenium Catalyzed Oxidative Cleavage of High Oleic Sunflower Oil: Considerations Regarding the Synthesis of a Fully Biobased Triacid
Authors: Santos Correa Luis , Meier Michael A. R. 
Published: December 2022

AbstractTricarboxylic acids are molecules of interest for the synthesis of highly cross‐linked polymers, for instance, for the curing of epoxy resins. Herein, a synthesis route to a novel high oleic sunflower oil based triacid is described by applying a ruthenium catalyzed oxidative cleavage of its double bonds. A statistical concept is devised for the prediction of the yields of mono‐, di‐, and trifunctional derivatives that can be formed from high oleic sunflower oil, depending on the overall conversion of double bonds into this functional group and the overall oleic acid content of the used oil. This concept proved to be highly useful for the explanation of seemingly moderate triacid yields, which are inherently dependent on the unsaturated fatty acid content of the used oil. All obtained sunflower oil based polyacids are fully analyzed by attenuated total reflection infrared spectroscopy (ATR‐IR), electrospray ionization mass spectrometry (ESI‐MS), 1H, 13C, and quantitative 31P nuclear magnetic resonance (NMR) spectroscopy. In addition, a more sustainable purification procedure is developed to obtain a polymerizable mixture of polyacids containing more than 2.0 carboxylic acids per molecule in average.Practical applications: Tricarboxylic acids are valuable monomers for the synthesis of cross‐linked polymers. The herein reported procedure represents a hitherto unknown synthesis route towards a new triacid and polyacid mixture directly from high oleic sunflower oil.
Antagonistic effect of kale soluble dietary fiber and kale flavonoids, fails to alleviate colitis
Authors: Wang Hui , Huang Xiaojun , Xia Shengkun , Chen Xiaomin , Chen Chunhua , Zhang Yanli , Xiao Jianbo , Nie Shaoping 
Published: December 2022

AbstractKale has been reported to have potential alleviating effects on colitis. However, the functional activities of kale dietary fibers and kale flavonoids (KF), as well as the effects of their combined use, were currently unknown. In this study, the efficacy of whole kale, kale soluble dietary fiber (KSDF), kale insoluble dietary fiber (KIDF), KF, mixture of KSDF and KF, and mixture of KIDF and KF on dextran sulfate sodium‐induced colitis mice was assessed to dissect which component(s) play the key role in alleviating colitis. The study demonstrated that administration of KSDF, KIDF, or KF resulted in various degrees of beneficial effects, including reduction of weight loss, colonic shortening, disease activity index score, and mRNA levels of inflammatory factors (TNF‐α, IL‐6, IL‐1β, and IL‐33), increase of mucin expression and suppression of intestinal g_Sutterella, g_Parabacteroides, f_Enterobacteriaceae relative abundance. However, 20% mouse mortality was found in group of KSDF combined with KF. Further analysis found that the combination antagonized the alleviating effects of both agents alone on colitis in mice. It is the first to report that there is antagonism between KSDF and KF in colitis, and the regulation of intestinal flora may be the mechanism. In addition, this study also showed that KSDF, KIDF, and KF alone had excellent effects to alleviate colitis. This study provides a new perspective and evidence to explore the effect of interaction among different food nutrients on intestinal inflammation.
Effect of auto‐exhaust pollution on leaf anatomy and micromorphology of Pistacia vera L.
Authors: Açar Mikail 
Published: December 2022

AbstractOne of the important causes of air pollution is automobile emissions Automobile exhausts can be toxic and harmful to plants. In light of this, this study aimed to examine the anatomical and micromorphological effects of auto exhaust pollution on Pistacia vera L. (Pistachio), an important agricultural plant. Pistachio leaflet samples taken from heavy traffic areas and rural areas were compared anatomically and micromorphologically. Also, the data were statistically evaluated. As a result of the study, it was seen that there are anatomical and micromorphological differences between the plant in the rural area and the plant in the heavy traffic area in the city center where air pollution and the number of cars are high. In particular, significant differences were detected in the epidermis surface and cuticle structure, trichome cover, stomatal characteristics, as well as mesophyll width, and epidermis anticlinal outer wall width. As a result, the study revealed that the agriculturally important P. vera is affected by pollution anatomically and micromorphologically.
Cell‐specific targeting of extracellular vesicles through engineering the glycocalyx
Authors: Zheng Wenyi , He Rui , Liang Xiuming , Roudi Samantha , Bost Jeremy , Coly Pierre‐Michael , van Niel Guillaume , Andaloussi Samir E. L. 
Published: December 2022

AbstractExtracellular vesicles (EVs) are promising carriers for the delivery of a variety of chemical and biological drugs. However, their efficacy is limited by the lack of cellular specificity. Available methods to improve the tissue specificity of EVs predominantly rely on surface display of proteins and peptides, largely overlooking the dense glycocalyx that constitutes the outermost layer of EVs. In the present study, we report a reconfigurable glycoengineering strategy that can endogenously display glycans of interest on EV surface. Briefly, EV producer cells are genetically engineered to co‐express a glycosylation domain (GD) inserted into the large extracellular loop of CD63 (a well‐studied EV scaffold protein) and fucosyltransferase VII (FUT7) or IX (FUT9), so that the engineered EVs display the glycan of interest. Through this strategy, we showcase surface display of two types of glycan ligands, sialyl Lewis X (sLeX) and Lewis X, on EVs and achieve high specificity towards activated endothelial cells and dendritic cells, respectively. Moreover, the endothelial cell‐targeting properties of sLeX‐EVs were combined with the intrinsic therapeutic effects of mesenchymal stem cells (MSCs), leading to enhanced attenuation of endothelial damage. In summary, this study presents a reconfigurable glycoengineering strategy to produce EVs with strong cellular specificity and highlights the glycocalyx as an exploitable trait for engineering EVs.
A failure in the medication delivery system—how disclosure and systems investigation improve patient safety
Authors: Lucas Scott R. , Pollak Ed , Makowski Charles 
Published: December 2022

AbstractA recent medication error at Vanderbilt University Medical Center contributed to the death of a patient. The ensuing criminal indictment of the administering nurse has shaken the medical community. This has led to clinical staff questioning whether they can disclose patient safety incidents without fear of criminal prosecution. However, because of the publicity of this case, hospitals can benefit from the lessons learned and mitigate the risk of this and similar events at their facilities. To uncover the most impactful and relevant safety recommendations, the Vanderbilt case is examined from a systems investigation perspective using the available public information gathered from media reports, the Tennessee Bureau of Investigation report, and Vanderbilt's corrective action plan submitted to CMS. We present an example of how hospitals can benefit from disclosure: Henry Ford Health used the Vanderbilt case study as part of its medication safety continuous improvement initiatives, which are underpinned by available medication safety recommendations from the Institute for Safe Medication Practices. Using this experience and the lessons learned from the Vanderbilt case, a proactive action plan is presented for hospitals nationwide to prevent the recurrence of this medication error. Without disclosure, these analyses and safety recommendations would not have been possible.
Whole‐genome RNA sequencing identifies distinct transcriptomic profiles in impingement cartilage between patients with femoroacetabular impingement and hip osteoarthritis
Authors: Kuhns Benjamin D. , Reuter John M. , Hansen Victoria L. , Soles Gillian L. , Jonason Jennifer H. , Ackert‐Bicknell Cheryl L. , Wu Chia‐Lung , Giordano Brian D. 
Published: December 2022

AbstractFemoroacetabular impingement (FAI) has a strong clinical association with the development of hip osteoarthritis (OA); however, the pathobiological mechanisms underlying the transition from focal impingement to global joint degeneration remain poorly understood. The purpose of this study is to use whole‐genome RNA sequencing to identify and subsequently validate differentially expressed genes (DEGs) in femoral head articular cartilage samples from patients with FAI and hip OA secondary to FAI. Thirty‐seven patients were included in the study with whole‐genome RNA sequencing performed on 10 gender‐matched patients in the FAI and OA cohorts and the remaining specimens were used for validation analyses. We identified a total of 3531 DEGs between the FAI and OA cohorts with multiple targets for genes implicated in canonical OA pathways. Quantitative reverse transcription‐polymerase chain reaction validation confirmed increased expression of FGF18 and WNT16 in the FAI samples, while there was increased expression of MMP13 and ADAMTS4 in the OA samples. Expression levels of FGF18 and WNT16 were also higher in FAI samples with mild cartilage damage compared to FAI samples with severe cartilage damage or OA cartilage. Our study further expands the knowledge regarding distinct genetic reprogramming in the cartilage between FAI and hip OA patients. We independently validated the results of the sequencing analysis and found increased expression of anabolic markers in patients with FAI and minimal histologic cartilage damage, suggesting that anabolic signaling may be increased in early FAI with a transition to catabolic and inflammatory gene expression as FAI progresses towards more severe hip OA. Clinical significance:Cam‐type FAI has a strong clinical association with hip OA; however, the cellular pathophysiology of disease progression remains poorly understood. Several previous studies have demonstrated increased expression of inflammatory markers in FAI cartilage samples, suggesting the involvement of these inflammatory pathways in the disease progression. Our study further expands the knowledge regarding distinct genetic reprogramming in the cartilage between FAI and hip OA patients. In addition to differences in inflammatory gene expression, we also identified differential expression in multiple pathways involved in hip OA progression.
Psychosocial impacts of the COVID‐19 pandemic on women with trauma histories: Study of Women's Health Across the Nation (SWAN)
Authors: Jakubowski Karen P. , Koffer Rachel E. , Matthews Karen A. , Burnett‐Bowie Sherri‐Ann M. , Derby Carol A. , Yu Elaine W. , Green Robin , Thurston Rebecca C. 
Published: December 2022

AbstractOlder adults, particularly those with trauma histories, may be vulnerable to adverse psychosocial outcomes during the COVID‐19 pandemic. We tested associations between prepandemic childhood abuse or intimate partner violence (IPV) and elevated depressive, anxiety, conflict, and sleep symptoms during the pandemic among aging women. Women (N = 582, age: 65–77 years) from three U.S. sites (Pittsburgh, Boston, Newark) of the longitudinal Study of Women's Health Across the Nation (SWAN) reported pandemic‐related psychosocial impacts from June 2020–March 2021. Prepandemic childhood abuse; physical/emotional IPV; social functioning; physical comorbidities; and depressive, anxiety, and sleep symptoms were drawn from SWAN assessments between 2009 and 2017. There were no measures of prepandemic conflict. In total, 47.7% and 35.3% of women, respectively, reported childhood abuse or IPV. Using logistic regression models adjusted for age; race/ethnicity; education; site; prepandemic social functioning and physical comorbidities; and, in respective models, prepandemic depressive, anxiety, or sleep symptoms, childhood abuse predicted elevated anxiety symptoms, OR = 1.67, 95% CI [1.10, 2.54]; household conflict, OR = 2.19, 95% CI [1.32, 3.61]; and nonhousehold family conflict, OR = 2.14, 95% CI [1.29, 3.55]. IPV predicted elevated sleep problems, OR = 1.63, 95% CI [1.07, 2.46], and household conflict, OR = 1.96, 95% CI [1.20, 3.21]. No associations emerged for depressive symptoms after adjusting for prepandemic depression. Aging women with interpersonal trauma histories reported worse anxiety, sleep, and conflict during the COVID‐19 pandemic than those without. Women's trauma histories and prepandemic symptoms are critical to understanding the psychosocial impacts of the pandemic.
An empirical examination of SMEs sustainable performance through lean manufacturing
Authors: Anwar Aliya , Jamil Khalid , Idrees Muhammad , Atif Muhammad , Ali Basharat 
Published: December 2022

AbstractIn the modern business world, manufacturing entities seek the modern way of production, which can help them to minimize the wastage of material. To minimize the wastage of materials in manufacturing entities, use different modern techniques, and one of the modern techniques is lean manufacturing. This study aims to explore the relationship between lean manufacturing and the sustainable performance (SP) of the firm. For this purpose, data were collected from SMEs working in Pakistan. Results elaborate that four lean manufacturing practices, that is, process and equipment, product design, supplier relationship, and customer relationship have a positive and significant impact on SP, but the impact of planning and control and HR practices could not be verified. Moreover, green supply chain management (GSCM) found a mediator between HR practices, product design, supplier relationships, customer relationships, and SP. Outcomes of this study provide the higher management of SMEs with a way forward to implement modern techniques within their organizations to minimize the wastage of material during the manufacturing process. Furthermore, this study will cover the deficiency in the literature about the mediating role of GSCM between lean manufacturing and sustainable performance.
On the generic behavior of the metric entropy, and related quantities, of uniformly continuous maps over Polish metric spaces
Authors: Carvalho Silas L. , Condori Alexander 
Published: December 2022

AbstractIn this work, we show that if f is a uniformly continuous map defined over a Polish metric space, then the set of f‐invariant measures with zero metric entropy is a set (in the weak topology). In particular, this set is generic if the set of f‐periodic measures is dense in the set of f‐invariant measures. This settles a conjecture posed by Sigmund (Trans. Amer. Math. Soc. 190 (1974), 285–299), which states that the metric entropy of an invariant measure of a topological dynamical system that satisfies the periodic specification property is typically zero. We also show that if X is compact and if f is an expansive or a Lipschitz map with a dense set of periodic measures, typically the lower correlation entropy for is equal to zero. Moreover, we show that if X is a compact metric space and if f is an expanding map with a dense set of periodic measures, then the set of invariant measures with packing dimension, upper rate of recurrence and upper quantitative waiting time indicator equal to zero is residual.
The weak Stokes problem associated with a flow through a profile cascade in Lr$L^r$‐framework
Authors: Neustupa Tomáš 
Published: December 2022

AbstractWe study the weak steady Stokes problem, associated with a flow of a Newtonian incompressible fluid through a spatially periodic profile cascade, in the ‐setup. The mathematical model used here is based on the reduction to one spatial period, represented by a bounded 2D domain Ω. The corresponding Stokes problem is formulated using three types of boundary conditions: the conditions of periodicity on the “lower” and “upper” parts of the boundary, the Dirichlet boundary conditions on the “inflow” and on the profile and an artificial “do nothing”‐type boundary condition on the “outflow.” Under appropriate assumptions on the given data, we prove the existence and uniqueness of a weak solution in and its continuous dependence on the data. We explain the sense in which the “do nothing” boundary condition on the “outflow” is satisfied.
Quantifying properties (K) and (μs$\mu ^{s}$)
Authors: Chen Dongyang , Kania Tomasz , Ruan Yingbin 
Published: December 2022

AbstractA Banach space X has property (K), whenever every weak* null sequence in the dual space admits a convex block subsequence so that as for every weakly null sequence in X; X has property if every weak* null sequence in admits a subsequence so that all of its subsequences are Cesàro convergent to 0 with respect to the Mackey topology. Both property and reflexivity (or even the Grothendieck property) imply property (K). In this paper, we propose natural ways for quantifying the aforementioned properties in the spirit of recent results concerning other familiar properties of Banach spaces.