bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bring it on: Top five antimicrobial stewardship challenges in transplant infectious diseases and practical strategies to address them
Authors: So Miranda , Tsai Helen , Swaminathan Neeraja , Bartash Rachel 
Published: April 2022

AbstractAntimicrobial therapies are essential tools for transplant recipients who are at high risk for infectious complications. However, judicious use of antimicrobials is critical to preventing the development of antimicrobial resistance. Treatment of multidrug-resistant organisms is challenging and potentially leads to therapies with higher toxicities, intravenous access, and intensive drug monitoring for interactions. Antimicrobial stewardship programs are crucial in the prevention of antimicrobial resistance, though balancing these strategies with the need for early and frequent antibiotic therapy in these immunocompromised patients can be challenging. In this review, we summarize 5 frequently encountered transplant infectious disease stewardship challenges, and we suggest strategies to improve practices for each clinical syndrome. These 5 challenging areas are: asymptomatic bacteriuria in kidney transplant recipients, febrile neutropenia in hematopoietic stem cell transplantation, antifungal prophylaxis in liver and lung transplantation, treatment of left-ventricular assist device infections, andClostridioides difficileinfection in solid-organ and hematopoietic stem-cell transplant recipients. Common themes contributing to these challenges include limited data specific to transplant patients, shortcomings in diagnostic testing, and uncertainties in pharmacotherapy.
Association between protein intake and lean body mass in a group of Masters Athletes
Authors: Stanzione Joseph R. , Boullata Joseph I. , Bruneau Michael L. , Volpe Stella L. 
Published: April 2022

Abstract
Recommendations for protein intake are based on total body weight; however, these recommendations do not consider lean body mass (LBM). The purpose of the present study was to identify the average protein intake in g/kg LBM in a group of healthy Masters Athletes (≥26 years of age, exercising ≥2 d/week). Data were obtained from a cross-sectional study. Body weight (kg), height (cm) and LBM via dual-energy X-ray absorptiometry were measured. Dietary intake was measured using a 2005 Block Food Frequency Questionnaire. The average energy intake, the percent energy from protein and the average protein intake in g/kg LBM were calculated. Differences between protein intake and the US Recommended Dietary Allowance (US RDA) (0⋅8 g/kg body weight) were determined. Alpha levels were set a priori to P < 0⋅05. A total of 176 participants (94 women, 82 men; 39 ± 11 years of age; body mass index: 24⋅6 ± 3⋅4 kg/m2) were analysed. The average energy intake, the percent protein energy and the average protein intake were 7996⋅9 ± 110⋅9 kilojoules (kJ)/d (1,910⋅4 ± 26⋅5 kcal), 15⋅5 ± 2⋅6 % and 1⋅43 ± 0⋅53 g/kg LBM, respectively. No differences existed between women and men for protein intake/kg LBM. Both sexes had significantly higher protein intakes than the US RDA (P < 0⋅001). We identified the average protein intake (g/kg LBM) in healthy Masters Athletes that may contribute to evolving perspectives on the determination of protein needs. The present study helps establish the relationship between protein intake and LBM so that we may further increase our accuracy when developing future protein recommendations.
Drawing a Line in the Sand
Authors: Wodak Josh 
Published: April 2022

What conservation could possibly become commensurate with the rates of human-induced biophysical change unfolding at the advent to the Sixth Extinction Event? Any such conservation would require time-critical interventions into both ecosystems and evolution itself, for these interventions would also require domains of risk and ethics that shatter normative understandings of conservation. Yet a line appears to have been drawn in the sand against such experimental conservation. Holding the line will retain conservation practices that are null and void against the extinction debt facing multitudes of species. Crossing the line would invoke scales of bioengineering that appear abhorrent to normative morality. This article explores the question of whether this line in the sand could, and should, be crossed through a detailed case study of current and proposed conservation for endangered Chelonia mydas sea turtles on Raine Island, a small coral cay on the Great Barrier Reef in Australia. Chelonia mydas and Raine Island are presented as synecdoche for conservation across diverse species across the world because turtles are among the most endangered of all reptiles and Raine Island is the largest and most important rookery in the world for this species. With such lines disappearing under the rising seas, the article contemplates the unthinkable questions that our current situation demands we ask, and perhaps even try to answer.
Self-perceptions of ageing and perceived health status: the mediating role of cognitive functioning and physical activity
Authors: Fernández-Jiménez Carolina , Dumitrache Cristina G. , Rubio Laura , Ruiz-Montero Pedro J. 
Published: April 2022

AbstractThere is evidence that perceived health status is relevant for older adults' wellbeing, and at the same time perceived health status can be influenced by self-perceptions of ageing. There is a lack of studies on the relationship between these variables. The aim of this study was to explore attitudes towards ageing and self-perceptions of ageing among Spanish middle-aged and older people, and to identify possible mediators in the relationship between self-perceptions of ageing on perceived health status. The sample comprised 1,124 individuals from 50 to 98 years old (mean = 64.84, standard deviation = 10.12) from the Ageing in Spain Longitudinal Study database, Pilot Survey (ELES-PS). Almost 70 per cent of the participants stated that old age begins at a specific chronological age and half of them considered that society treats older people with indifference. Self-perceptions of ageing, physical activity, cognitive functioning and age were significant predictors, and together these variables explained 15.9 per cent in the variance of perceived health status. The multiple mediation analysis revealed that self-perceptions of ageing predicted cognitive functioning (B = 0.848, p < 0.01) and physical activity (B = 2.9046, p < 0.001), and mediated the association between self-perceptions of ageing and perceived health status (B = 0.1144, standard error = 0.0147, p < 0.01). Results from this study highlight the importance of perceptions and attitudes towards ageing for older adults' health and wellbeing, thus this study contributes to a better understanding of how these variables are related. In this sense, results from this study can be useful for designing interventions for fostering wellbeing. Addressing negative self-perceptions of ageing and negative attitudes towards ageing can be particularly useful because they are associated with more pessimistic expectancies about the ageing process.
Spatial meta-analysis of the occurrence and distribution of tsetse-transmitted animal trypanosomiasis in Cameroon over the last 30 years
Authors: Sevidzem Silas Lendzele , Koumba Aubin Armel , Mavoungou Jacques François , Windsor Peter Andrew 
Published: April 2022

Abstract
In Cameroon, >90% of cattle are considered exposed to African animal trypanosomiasis (AAT) infection, with the presence of tsetse rendering cattle husbandry as a very difficult proposition. A systematic review of data on AAT and tsetse from 1990 to 2021 was conducted to develop a national atlas. The review identified 74 relevant scientific documents, with three pathogenic Trypanosoma species (Trypanosoma vivax, T. congolense and T. brucei s.l.) most frequently identified as causing AAT. Trypanosoma grayi, T. theileri, T. simiae and the human African trypanosomiasis causative agent T. brucei gambiense were also identified in a wide range of hosts. The tsetse fly fauna of Cameroon comprises nine species, with Glossina palpalis palpalis and G. fuscipes fuscipes the most widely distributed following their identification in seven and five of the 10 regions, respectively. Two species, Glossina nigrofusca and G. pallicera pallicera appeared to be rare and were restricted to both forest and protected areas. The presence of AAT is associated with the presence of tsetse in the livestock–human–wildlife interface of Cameroon. AAT occurs beyond the tsetse belts of the country where mechanical vectors are abundant. This study provides AAT and tsetse maps to support ongoing interventions in Cameroon.
Development of a millimeter-wave transparent antenna inside a headlamp for automotive radar application
Authors: Hamid Sofian , Heberling Dirk , Junghähnel Manuela , Preussner Thomas , Gretzki Patrick , Pongratz Ludwig , Hördemann Christian , Gillner Arnold 
Published: April 2022

AbstractThe development of a millimeter-wave transparent antenna integrated inside a headlamp for automotive radar application is presented. The antenna consists of two radiating elements: the primary and secondary ones. The primary antenna is the one that is fabricated on RF PCB material (e.g., patch, slot, sectoral horn) and connected directly to the transceiver chip, while the secondary antenna is made of optically transparent materials such as glass, but with a optical transparent electrically conductive coating, well known as transparent conductive oxide (TCO). This antenna is realized as a planar offset reflector to collimate and shape the incoming wave from the primary antenna. This reflector is designed based on the Fresnel theory and the reflectarray concept. The division of the primary and secondary antenna enables the placement of the radar module (that contains the primary antenna) at the base of the headlamp, and therefore it is concealed from the surroundings and hidden from the optical path of the light. The secondary antenna is inserted in the space between the headlamp cover and the light unit. The main challenge here is to provide a maximum on transparency in the visible range of the spectrum with a specially designed and laser-based generated microstructure for the resonant reflection of the radar wavelength. An antenna demonstrator has been fabricated, and together with the headlamp cover, the radiation pattern and realized gain are measured. We reported here the measurement results for several reflector designs and concluded that the headlamp cover gives minimal influence on the antenna performance.
Subsurface Eddy Facilitates Retention of Simulated Diel Vertical Migrators in a Biological Hotspot
Authors: Hudson K. , Oliver M. J. , Kohut J. , Cohen J. H. , Dinniman M. S. , Klinck J. M. , Reiss C. S. , Cutter G. R. , Statscewich H. , Bernard K. S. , Fraser W. 
Published: April 2022

AbstractDiel vertical migration (DVM) is common in zooplankton populations worldwide. Every day, zooplankton leave the productive surface ocean and migrate to deepwater to avoid visual predators and return to the surface at night to feed. This behavior may also help retain migrating zooplankton in biological hotspots. Compared to fast and variable surface currents, deep ocean currents are sluggish, and can be more consistent. The time spent in the subsurface layer is driven by day length and the depth of the surface mixed layer. A subsurface, recirculating eddy has recently been described in Palmer Deep Canyon (PDC), a submarine canyon in a biological hotspot located adjacent to the West Antarctic Peninsula. Circulation model simulations have shown that residence times of neutrally buoyant particles increase with depth within this feature. We hypothesize that DVM into the subsurface eddy increases local retention of migrating zooplankton in this feature and that shallow mixed layers and longer days increase residence times. We demonstrate that simulated vertically migrating zooplankton can have residence times on the order of 30 days over the canyon, which is five times greater than residence times of near‐surface, nonmigrating zooplankton within PDC and other adjacent coastal regions. The potential interaction of zooplankton with this subsurface feature may be important to the establishment of the biological hotspot around PDC by retaining food resources in the region. Acoustic field observations confirm the presence of vertical migrators in this region, suggesting that zooplankton retention due to the subsurface eddy is feasible.
Thermohydrological Impact of Forest Disturbances on Ecosystem‐Protected Permafrost
Authors: Stuenzi Simone Maria , Kruse Stefan , Boike Julia , Herzschuh Ulrike , Oehme Alexander , Pestryakova Luidmila A. , Westermann Sebastian , Langer Moritz 
Published: April 2022

AbstractBoreal forests cover over half of the global permafrost area and protect underlying permafrost. Boreal forest development, therefore, has an impact on permafrost evolution, especially under a warming climate. Forest disturbances and changing climate conditions cause vegetation shifts and potentially destabilize the carbon stored within the vegetation and permafrost. Disturbed permafrost‐forest ecosystems can develop into a dry or swampy bush‐ or grasslands, shift toward broadleaf‐ or evergreen needleleaf‐dominated forests, or recover to the pre‐disturbance state. An increase in the number and intensity of fires, as well as intensified logging activities, could lead to a partial or complete ecosystem and permafrost degradation. We study the impact of forest disturbances (logging, surface, and canopy fires) on the thermal and hydrological permafrost conditions and ecosystem resilience. We use a dynamic multilayer canopy‐permafrost model to simulate different scenarios at a study site in eastern Siberia. We implement expected mortality, defoliation, and ground surface changes and analyze the interplay between forest recovery and permafrost. We find that forest loss induces soil drying of up to 44%, leading to lower active layer thicknesses and abrupt or steady decline of a larch forest, depending on disturbance intensity. Only after surface fires, the most common disturbances, inducing low mortality rates, forests can recover and overpass pre‐disturbance leaf area index values. We find that the trajectory of larch forests after surface fires is dependent on the precipitation conditions in the years after the disturbance. Dryer years can drastically change the direction of the larch forest development within the studied period.
Decoy receptor 3 is involved in epidermal keratinocyte commitment to terminal differentiation via EGFR and PKC activation
Authors: Wu Nan-Lin , Huang Duen-Yi , Hsieh Shie-Liang , Dai Yang-Shia , Lin Wan-Wan 
Published: April 2022

AbstractDecoy receptor 3 (DcR3) is a soluble receptor for Fas ligand, LIGHT and TL1A, but it also exerts effector functions. Previously, we found that DcR3 is upregulated in the serum and lesional skin of patients with psoriasis and is upregulated by EGFR activation in proliferating primary human epidermal keratinocytes. However, the functional role of intracellular DcR3 in keratinocyte differentiation is still incompletely defined. Herein, primary cultured human epidermal keratinocytes were differentiated by phorbol 12-myristate 13-acetate (PMA) treatment, calcium treatment and cell confluence, which are three standard in vitro differentiation models. We found that the constitutive expression of the DcR3 gene and protein was progressively suppressed during terminal differentiation of keratinocytes. These changes were correlated with downregulation of EGFR activation during keratinocyte differentiation. EGFR inhibition by gefitinib further decreased confluence-induced suppression of DcR3 mRNA expression, and, vice versa, knocking down DcR3 expression attenuated EGFR and EGFR ligand expression as well as EGFR activation. Under conditions without a change in cell growth, DcR3 silencing reduced the expression of involucrin and transglutaminase 1 but enhanced the induction of the terminal differentiation markers keratin 10 and loricrin. Of note, DcR3 interacted with PKCα and PKCδ and enhanced PKC activity. In keratinocytes with PKCα and PKCδ silencing, differentiation markers were differentially affected. In conclusion, DcR3 expression in keratinocytes is regulated by EGFR and forms a positive feedback loop to orchestrate constitutive EGFR and PKC activity. During differentiation, DcR3 is downregulated and involved in modulating the pattern of terminal differentiation.
Structure and density of silicon carbide to 1.5 TPa and implications for extrasolar planets
Authors: Kim D. , Smith R. F. , Ocampo I. K. , Coppari F. , Marshall M. C. , Ginnane M. K. , Wicks J. K. , Tracy S. J. , Millot M. , Lazicki A. , Rygg J. R. , Eggert J. H. , Duffy T. S. 
Published: April 2022

AbstractThere has been considerable recent interest in the high-pressure behavior of silicon carbide, a potential major constituent of carbon-rich exoplanets. In this work, the atomic-level structure of SiC was determined through in situ X-ray diffraction under laser-driven ramp compression up to 1.5 TPa; stresses more than seven times greater than previous static and shock data. Here we show that the B1-type structure persists over this stress range and we have constrained its equation of state (EOS). Using this data we have determined the first experimentally based mass-radius curves for a hypothetical pure SiC planet. Interior structure models are constructed for planets consisting of a SiC-rich mantle and iron-rich core. Carbide planets are found to be ~10% less dense than corresponding terrestrial planets.
Peptidoglycan biosynthesis is driven by lipid transfer along enzyme-substrate affinity gradients
Authors: Oluwole Abraham O. , Corey Robin A. , Brown Chelsea M. , Hernández-Rocamora Victor M. , Stansfeld Phillip J. , Vollmer Waldemar , Bolla Jani R. , Robinson Carol V. 
Published: April 2022

AbstractMaintenance of bacterial cell shape and resistance to osmotic stress by the peptidoglycan (PG) renders PG biosynthetic enzymes and precursors attractive targets for combating bacterial infections. Here, by applying native mass spectrometry, we elucidate the effects of lipid substrates on the PG membrane enzymes MraY, MurG, and MurJ. We show that dimerization of MraY is coupled with binding of the carrier lipid substrate undecaprenyl phosphate (C55-P). Further, we demonstrate the use of native MS for biosynthetic reaction monitoring and find that the passage of substrates and products is controlled by the relative binding affinities of the different membrane enzymes. Overall, we provide a molecular view of how PG membrane enzymes convey lipid precursors through favourable binding events and highlight possible opportunities for intervention.
Data-driven capacity estimation of commercial lithium-ion batteries from voltage relaxation
Authors: Zhu Jiangong , Wang Yixiu , Huang Yuan , Bhushan Gopaluni R. , Cao Yankai , Heere Michael , Mühlbauer Martin J. , Mereacre Liuda , Dai Haifeng , Liu Xinhua , Senyshyn Anatoliy , Wei Xuezhe , Knapp Michael , Ehrenberg Helmut 
Published: April 2022

AbstractAccurate capacity estimation is crucial for the reliable and safe operation of lithium-ion batteries. In particular, exploiting the relaxation voltage curve features could enable battery capacity estimation without additional cycling information. Here, we report the study of three datasets comprising 130 commercial lithium-ion cells cycled under various conditions to evaluate the capacity estimation approach. One dataset is collected for model building from batteries with LiNi0.86Co0.11Al0.03O2-based positive electrodes. The other two datasets, used for validation, are obtained from batteries with LiNi0.83Co0.11Mn0.07O2-based positive electrodes and batteries with the blend of Li(NiCoMn)O2- Li(NiCoAl)O2positive electrodes. Base models that use machine learning methods are employed to estimate the battery capacity using features derived from the relaxation voltage profiles. The best model achieves a root-mean-square error of 1.1% for the dataset used for the model building. A transfer learning model is then developed by adding a featured linear transformation to the base model. This extended model achieves a root-mean-square error of less than 1.7% on the datasets used for the model validation, indicating the successful applicability of the capacity estimation approach utilizing cell voltage relaxation.
Safety and serum distribution of anti-SARS-CoV-2 monoclonal antibody MAD0004J08 after intramuscular injection
Authors: Lanini Simone , Milleri Stefano , Andreano Emanuele , Nosari Sarah , Paciello Ida , Piccini Giulia , Gentili Alessandra , Phogat Adhuna , Hyseni Inesa , Leonardi Margherita , Torelli Alessandro , Montomoli Emanuele , Paolini Andrea , Frosini Andrea , Antinori Andrea , Nicastri Emanuele , Girardi Enrico , Plazzi Maria Maddalena , Ippolito Giuseppe , Vaia Francesco , Della Cioppa Giovanni , Rappuoli Rino 
Published: April 2022

AbstractThe emerging threat represented by SARS-CoV-2 variants, demands the development of therapies for better clinical management of COVID-19. MAD0004J08 is a potent Fc-engineered monoclonal antibody (mAb) able to neutralize in vitro all current SARS-CoV-2 variants of concern (VoCs) including the omicron variant even if with significantly reduced potency. Here we evaluated data obtained from the first 30 days of a phase 1 clinical study (EudraCT N.: 2020-005469-15 and ClinicalTrials.gov Identifier: NCT04932850). The primary endpoint evaluated the percentage of severe adverse events. Secondary endpoints evaluated pharmacokinetic and serum neutralization titers. A single dose administration of MAD0004J08 via intramuscular (i.m.) route is safe and well tolerated, resulting in rapid serum distribution and sera neutralizing titers higher than COVID-19 convalescent and vaccinated subjects. A single dose administration of MAD0004J08 is also sufficient to effectively neutralize major SARS-CoV-2 variants of concern (alpha, beta, gamma and delta). MAD0004J08 can be a major advancement in the prophylaxis and clinical management of COVID-19.
A cryptic pocket in Ebola VP35 allosterically controls RNA binding
Authors: Cruz Matthew A. , Frederick Thomas E. , Mallimadugula Upasana L. , Singh Sukrit , Vithani Neha , Zimmerman Maxwell I. , Porter Justin R. , Moeder Katelyn E. , Amarasinghe Gaya K. , Bowman Gregory R. 
Published: April 2022

AbstractProtein-protein and protein-nucleic acid interactions are often considered difficult drug targets because the surfaces involved lack obvious druggable pockets. Cryptic pockets could present opportunities for targeting these interactions, but identifying and exploiting these pockets remains challenging. Here, we apply a general pipeline for identifying cryptic pockets to the interferon inhibitory domain (IID) of Ebola virus viral protein 35 (VP35). VP35 plays multiple essential roles in Ebola’s replication cycle but lacks pockets that present obvious utility for drug design. Using adaptive sampling simulations and machine learning algorithms, we predict VP35 harbors a cryptic pocket that is allosterically coupled to a key dsRNA-binding interface. Thiol labeling experiments corroborate the predicted pocket and mutating the predicted allosteric network supports our model of allostery. Finally, covalent modifications that mimic drug binding allosterically disrupt dsRNA binding that is essential for immune evasion. Based on these results, we expect this pipeline will be applicable to other proteins.