bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D object tracker for sports events
Authors: Volkova M. A. , Romanov M. P. , Bychkov A. M. 
Published: October 2022

Objectives. Sports events are currently among the most promising areas for the application of tracking systems. In most cases, such systems are designed to track moving objects in a two-dimensional plane, e.g., players on the field, as well as to identify them by various features. However, as new sports such as drone racing are developed, the problem of determining the position of an object in a three-dimensional coordinate system becomes relevant. The aim of the present work was to develop algorithms and software for a method to perform 3D tracking of moving objects, regardless of the data segmentation technique, and to test this method to estimate the tracking quality.Methods. A method for matching information on the speed and position of objects was selected based on a review and analysis of contemporary tracking methods.Results. The structure of a set of algorithms comprising software for a moving-object tracker for sports events is proposed. Experimental studies were performed on the publicly available APIDIS dataset, where a MOTA metric of 0.858 was obtained. The flight of an FPV quadcopter along a track was also tracked according to the proposed dataset; the 3D path of the drone flight was reconstructed using the tracker data.Conclusions. The results of the experimental studies, which demonstrated the feasibility of using the proposed method to track a quadcopter flight trajectory in a three-dimensional world coordinate system, is also showed that the method is suitable for tracking objects at sports events.
Technology for the creation of ferroelectric regular domain structures using interfering elastic waves
Authors: Krutov V. V. , Sigov A. S. 
Published: October 2022

Objectives. In many laboratories around the world, work is underway in the field of domain engineering of ferroelectrics. For a number of years, RTU MIREA has been conducting research on the creation of a high-performance technology for the formation of ferroelectric photonic and phononic crystals. The technology is characterized by a short duration of the technological cycle and provides the necessary depth of spatially periodic domain inversion. The key element of the technology is the combined effect of a uniform electric field and interfering high-frequency elastic waves that create a temperature grating. The technology is universal in relation to ferroelectrics of varying degrees of acoustic transparency, which is achieved by using highly dissipative liquid electrodes of a certain thickness. In this case, the energy of elastic waves practically does not penetrate into the ferroelectric, so the manifestation of undesirable effects is excluded. The purpose of this review article is to analyze the results of work carried out at RTU MIREA in the field of technology for the formation of ferroelectric regular domain structures (RDSs) during the period from 2008 to the present.Methods. Provisions of the theory of propagation, refraction and interference of elastic waves in condensed media are used, in particular, the Newtonian model of a liquid as applied to shear waves, as well as computer simulation. When considering the main stages of the Double Pulse heterothermal technology for the formation of RDSs, methods of analysis and synthesis were applied.Results. The possibility of forming not only micro-, but also submicron RDSs is shown. Recommendations are given on the choice of the type and specific properties of liquid electrodes, the angles between the direction of propagation of interfering waves, and their frequency. It is shown, in particular, that the use of highly dissipative ionic liquids as liquid electrodes creates favorable conditions for the formation of an RDS with a short period at room temperature. Thus, on shear waves with electrodes based on LiPF6-PC at a frequency of 300 MHz, RDS with a period of about 2 цт can be created. The main technological parameters are determined both for the case of the action of longitudinal elastic waves and for the case of shear waves with horizontal polarization. The results are applicable to ferroelectrics such as lithium niobate, potassium titanyl phosphate, and lead zirconate titanate.Conclusions. The proposed and studied methods are focused on the mass production of devices based on RDSs, in particular, on the manufacturing of optical parametric oscillators, acoustoelectronic devices, as well as terahertz wave generators and second harmonic oscillators. The technology has a short duration of the technological cycle, comparable to the polarization switching time in the used ferroelectric.
Factibilidad de una empresa comercializadora de artículos ortopédicos en la provincia de Cañar- Ecuador
Authors: Parra Quizhpi Luis Marcos , Ormaza Andrade Jorge Edwin 
Published: October 2022

La presente investigación se centra en analizar la conveniencia de implementar una casa comercializadora de artículos ortopédicos en la provincia de Cañar y la aceptación que tendría la misma. Con relación a su orientación metodológica su diseño fue exploratorio y descriptivo, a su vez transversal, no experimental, no probabilístico y con un enfoque cuantitativo, además se basó en la revisión sistemática de la literatura haciendo uso de las bases de datos científicas. Para la recopilación de datos se aplicó la técnica la encuesta y como instrumento el cuestionario, a una muestra de la Población Económicamente Activa (PEA) de Cañar, mediante el cual se observó que la empresa comercializadora de artículos ortopédicos es viable teniendo mayor aceptación en la ciudad de Azogues. Así también se propone el método de reconocer, oportunidad, tecnología, alianza y verificar (ROTAV) que aporta con fases a considerar en una idea de negocio, ofreciendo pautas a los empresarios o emprendedores mismo que ayudarán a potenciar el bien o servicio.
Shexiang Baoxin Pill (MUSKARDIA) reduces major adverse cardiovascular events in women with stable coronary artery disease: A subgroup analysis of a phase IV randomized clinical trial
Authors: Shi Haiming , Zhou Jingmin , Ma Changsheng , Ji Fusui , Wu Yang , Zhao Yulan , Qian Jun , Wang Xiaolong 
Published: October 2022

BackgroundA previous phase IV trial revealed sex as a potential effect modifier of MUSKARDIA efficacy in stable coronary artery disease (CAD).ObjectiveTo assess the clinical effect of MUSKARDIA as a supplemental treatment to optimal medical therapy (OMT) in stable CAD cases.MethodsThis study was a subgroup analysis of a multicenter, randomized, double-blinded, placebo-controlled phase IV clinical study. Eligible individuals underwent randomization to the oral MUSKARDIA and placebo groups and were treated for 24 months. All participants received OMT according to existing guidelines. The primary composite outcome was the major adverse cardiovascular event (MACE), included cardiovascular death, non-fatal myocardial infarction (MI), or non-fatal stroke. The secondary composite outcome encompassed all-cause mortality, non-fatal MI, non-fatal stroke, hospitalization for unstable angina and/or heart failure, and undergoing coronary procedure/surgery during treatment. Safety signals, especially cardiovascular adverse events (AEs), were analyzed.ResultsThe female subgroup included 776 participants (384 and 392 in the MUSKARDIA and placebo groups, respectively). The occurrence of the primary composite outcome was lower in the MUSKARDIA group compared with placebo-treated individuals (HR = 0.27, 95%CI: 0.09–0.83; P = 0.02), but the secondary composite outcome showed no significant difference (HR = 0.77, 95%CI: 0.47–1.25; P = 0.29). The MUSKARDIA group had reduced incidence of cardiovascular AEs compared with placebo-treated cases (2.9% vs. 5.6%).ConclusionAs a supplemental treatment to OMT, 24-month administration of MUSKARDIA is effective and safe in female stable CAD cases.Clinical trial registration[https://clinicaltrials.gov/], identifier [NCT01897805].
Tear function in patients with diabetes mellitus: A systematic review and meta-analysis
Authors: Kuo Yu-Kai , Shao Shih-Chieh , Lin Erh-Tsan , Pan Li-Yen , Yeung Ling , Sun Chi-Chin 
Published: October 2022

PurposeTo examine tear function in patients with diabetes mellitus (DM).DesignSystematic review and meta-analysis.MethodWe searched Embase and PubMed from database inception to March 16, 2022. We included observational studies that compared tear function between patients with and without DM. Tear function was measured using invasive tear breakup time (ITBUT) and Schirmer’s 1 test. Pooled results are presented as standard mean difference (SMD) with 95% confidence interval (CI) based on random-effects models.ResultsWe included 59 studies (7,234 eyes) comparing the tear function between patients with and without DM. This meta-analysis indicated that patients with DM had worse tear function than those without DM (ITBUT: SMD: −0.98, 95% CI: −1.27 to −0.69; Schirmer’s 1 test: SMD: −0.45, 95% CI: −0.64 to −0.26), and the results remained consistent in patients with different types of DM (e.g., type 1 DM and type 2 DM) and from different ethnic backgrounds (e.g., Asian vs. non-Asian). Patients with DM under poor glycemic control had worse tear function than those of the non-DM group (ITBUT: SMD: −1.26, 95% CI: −1.86 to −0.66; Schirmer’s 1 test: SMD: −0.25, 95% CI: −0.48 to −0.02), whereas there were no significant differences in tear function between patients with DM under optimal glycemic control and non-DM groups.ConclusionsWe found that patients with type 1 or type 2 DM had significantly reduced tear function. The level of tear function could be determined by glycemic control, and therefore, our findings suggest that glycemic control in patients with DM is critical for maintaining tear function.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero, identifier CRD42021250498.
Predicting the influence of Circ_0059706 expression on prognosis in patients with acute myeloid leukemia using classical statistics and machine learning
Authors: Ma Jichun , Wen Xiangmei , Xu Zijun , Xia Peihui , Jin Ye , Lin Jiang , Qian Jun 
Published: October 2022

Background: Various circular RNA (circRNA) molecules are abnormally expressed in acute myeloid leukemia (AML), and associated with disease occurrence and development, as well as patient prognosis. The roles of circ_0059706, a circRNA derived from ID1, in AML remain largely unclear.Results: Here, we reported circ_0059706 expression in de novo AML and its association with prognosis. We found that circ_0059706 expression was significantly lower in AML patients than in controls (p &lt; 0.001). Survival analysis of patients with AML divided into two groups according to high and low circ_0059706 expression showed that overall survival (OS) of patients with high circ_0059706 expression was significantly longer than that of those with low expression (p &lt; 0.05). Further, female patients with AML and those aged &gt;60 years old in the high circ_0059706 expression group had longer OS than male patients and those younger than 60 years. Multiple regression analysis showed that circ_0059706 was an independent factor-affecting prognosis of all patients with AML. To evaluate the prospects for application of circ_0059706 in machine learning predictions, we developed seven types of algorithm. The gradient boosting (GB) model exhibited higher performance in prediction of 1-year prognosis and 3-year prognosis, with AUROC 0.796 and 0.847. We analyzed the importance of variables and found that circ_0059706 expression level was the first important variables among all 26 factors included in the GB algorithm, suggesting the importance of circ_0059706 in prediction model. Further, overexpression of circ_0059706 inhibited cell growth and increased apoptosis of leukemia cells in vitro.Conclusion: These results provide evidence that high expression of circ_0059706 is propitious for patient prognosis and suggest circ_0059706 as a potential new biomarker for diagnosis and prognosis evaluation in AML, with high predictive value and good prospects for application in machine learning algorithms.
The beneficial effects of Lacticaseibacillus casei on the small intestine and colon of Swiss mice against the deleterious effects of 5-fluorouracil
Authors: Barbosa Stphannie Jamyla de Araújo , Oliveira Maisie Mitchele Barbosa , Ribeiro Susana Barbosa , de Medeiros Caroline Addison Carvalho Xavier , Lima Maria Laura de Souza , Guerra Gerlane Coelho Bernardo , de Araújo Júnior Raimundo Fernandes , de Sousa Junior Francisco Caninde , Martins Agnes Andrade , Paiva Daniel Felipe Fernandes , Andrade Raphael Victor Silva , Rebouças Conceição S. Martins , Brito Gerly Anne de Castro , Leitâo Renata Ferreira de Carvalho , de Araújo Aurigena Antunes 
Published: October 2022

BackgroundIntestinal mucositis is one of the most common and important side effects of 5-fluorouracil (5-FU). Currently, there are still no specific and effective protocols for its prevention and treatment. The aim of the present study was to evaluate the effect of oral administration of Lacticaseibacillus casei (L. casei) on the progression of 5-FU-induced intestinal mucositis. Methods: L. casei (1x109 CFU/ml) or saline was orally administered to Swiss mice, beginning 15 days before intestinal mucositis induction by single intraperitoneal 5-FU administration (450 mg/kg). Body weight, number of peripheral leukocytes and fecal lactic acid bacteria were monitored. After euthanasia, on day 18, tissue samples from colon and each small intestine segment were collected for histopathology. Jejunal tissues were collected and evaluated for iNOS and TNF-alpha immunoexpression, IL-1-beta, IL-6 and TNF-alpha levels, malonaldehyde (MDA) accumulation, invertase activity and factor nuclear kappa B (NFkB-P65) gene expression, toll like receptor-4 (TLR-4), mucin-2 (MUC-2), occludin and zonula occludens-1 (ZO-1).ResultsThe positive impact of L. casei on 5-FU-induced leukopenia was observed, but not on 5-FU-induced weight loss in mice. L. casei reduced 5-FU-induced inflammation in the colon and small intestine (p&lt;0.05). Decreased TNF-α, IL-1β, IL-6 (p&lt;0.05) and MDA (p&lt;0.05) levels, as well as decreased iNOS and TNF-alpha protein expressions (p&lt;0.05) were found in the jejunum from L casei group. In addition, L-casei down-regulated NFKB-P65 (p&lt;0.05) and TLR-4 (p&lt;0.05) gene expressions and up-regulated MUC-2 and mucosal barrier proteins occludin and ZO-1 gene expressions (p&lt;0.05). Furthermore, greater lactic acid bacteria population (p&lt;0.05) was found in the L. casei group when compared to control groups.ConclusionOral L. casei administration can protect the intestine of Swiss mice from 5-FU-induced intestinal mucositis, thus contributing to overall health.
Outcome predictors in COVID-19: An analysis of emergent systemic inflammation indices in Mexican population
Authors: Gutiérrez-Pérez Ilse Adriana , Buendía-Roldán Ivette , Pérez-Rubio Gloria , Chávez-Galán Leslie , Hernández-Zenteno Rafael de Jesus , Aguilar-Duran Hiram , Fricke-Galindo Ingrid , Zaragoza-García Oscar , Falfán-Valencia Ramcés , Guzmán-Guzmán Iris Paola 
Published: October 2022

IntroductionThe systemic viral disease caused by the SARS-CoV-2 called coronavirus disease 2019 (COVID-19) continues to be a public health problem worldwide.ObjectiveThis study is aimed to evaluate the association and predictive value of indices of systemic inflammation with severity and non-survival of COVID-19 in Mexican patients.Materials and MethodsA retrospective study was carried out on 807 subjects with a confirmed diagnosis of COVID-19. Clinical characteristics, acute respiratory distress syndrome (ARDS), severity according to PaO2/FiO2 ratio, invasive mechanical ventilation (IMV), and non-survival outcome were considered to assess the predictive value and the association of 11 systemic inflammatory indices derived from hematological parameters analyzed at the hospital admission of patients. The receiver operating characteristics curve was applied to determine the thresholds for 11 biomarkers, and their prognostic values were assessed via the Kaplan-Meier method.Results26% of the studied subjects showed COVID-19 severe (PaO2/FiO2 ratio ≤ 100), 82.4% required IMV, and 39.2% were non-survival. The indices NHL, NLR, RDW, dNLR, and SIRI displayed predictive values for severe COVID-19 and non-survival. NHL, SIRI, and NLR showed predictive value for IMV. The cut-off values for RDW (OR = 1.85, p &lt; 0.001), NHL (OR = 1.67, p = 0.004) and NLR (OR = 1.56, p = 0.012) were mainly associated with severe COVID-19. NHL (OR = 3.07, p &lt; 0.001), AISI (OR = 2.64, p &lt; 0.001) and SIRI (OR = 2.51, p &lt; 0.001) were associated with IMV support, while for non-survival the main indices associated were NHL (OR = 2.65, p &lt; 0.001), NLR (OR = 2.26, p &lt; 0.001), dNLR (OR = 1.92, p &lt; 0.001), SIRI (OR = 1.67, p = 0.002) and SII (OR = 1.50, p = 0.010). The patients with an RDW, PLR, NLR, dNLR, MLR, SII, and NHL above the cut-off had a survival probability of COVID-19 50% lower, with an estimated mean survival time of 40 days.ConclusionThe emergent systemic inflammation indices NHL, NLR, RDW, SII, and SIRI have a predictive power of severe COVID-19, IMV support, and low survival probability during hospitalization by COVID-19 in Mexican patients.
Improved chemical and isotopic labeling of biomembranes in Bacillus subtilis by leveraging CRISPRi inhibition of beta-ketoacyl-ACP synthase (fabF)
Authors: Nickels Jonathan D. , Bonifer Kyle S. , Tindall Rachel R. , Yahya Ahmad , Tan Luoxi , Do Changwoo , Davison Brian H. , Elkins James G. 
Published: October 2022

Assessing the structure of living microbial cell membranes is a challenging analytical goal. The cell membrane is defined by its transverse structure, an approximately 5 nm-thick selectively permeable bilayer that serves many important cellular functions. Compositionally complex, dynamic, and organized in both the transverse and lateral dimensions, understanding the cell membrane structure—and the role that structure plays in cellular function, communication, and environmental sensing is an active scientific effort. Previously, we have devised a novel isotopic labeling approach for membrane lipids to enable direct in vivo structural studies of the cell membrane in the Gram-positive bacterium, Bacillus subtilis, using small-angle neutron scattering. This was accomplished through a genetic inhibition of fatty acid (FA) degradation (ΔfadN) and a chemical inhibition of FA biosynthesis using cerulenin, an irreversible inhibitor of type II fatty acid synthases. Here, we improve upon the previous system by introducing a dCas9/sgRNA-fabF complex that blocks transcription of the essential fabF gene when under xylose induction. This leads to greater sensitivity to cerulenin in the mutant strain (JEBS102) and more robust cell growth when supplementary FAs are introduced to the culture medium. A subtle change in FA uptake is noted when compared to the prior labeling strategy. This is seen in the gas chromatography/mass spectrometry (GC/MS) data as a higher ratio of n16:0 to a15:0, and manifests in an apparent increase in the membrane thickness determined via neutron scattering. This represents an improved method of isotopic labeling for the cell membrane of Bacillus subtilis; enabling improved investigations of cellular uptake and utilization of FAs, cell membrane structure and organization as a phenotypic response to metabolic and environmental changes.
Construction of m6A-based prognosis signature and prediction for immune and anti-angiogenic response
Authors: Wang Xiang-Xu , Wu Li-Hong , Dou Qiong-Yi , Ai Liping , Lu Yajie , Deng Shi-Zhou , Liu Qing-Qing , Ji Hongchen , Zhang Hong-Mei 
Published: October 2022

Background: Increasing evidence illustrated that m6A regulator-mediated modification plays a crucial role in regulating tumor immune and angiogenesis microenvironment. And the combination of immune checkpoint inhibitor and anti-angiogenic therapy has been approved as new first-line therapy for advanced HCC. This study constructed a novel prognosis signature base on m6A-mediated modification and explored the related mechanism in predicting immune and anti-angiogenic responses.Methods: Gene expression profiles and clinical information were collected from TCGA and GEO. The ssGSEA, MCPCOUNT, and TIMER 2.0 algorithm was used to Estimation of immune cell infiltration. The IC50 of anti-angiogenic drugs in GDSC was calculated by the “pRRophetic” package. IMvigor210 cohort and Liu et al. cohort were used to validate the capability of immunotherapy response. Hepatocellular carcinoma single immune cells sequencing datasets GSE140228 were collected to present the expression landscapes of 5 hub genes in different sites and immune cell subpopulations of HCC patients.Results: Three m6A clusters with distinct immune and angiogenesis microenvironments were identified by consistent cluster analysis based on the expression of m6A regulators. We further constructed a 5-gene prognosis signature (termed as m6Asig-Score) which could predict both immune and anti-angiogenic responses. We illustrated that high m6Asig-Score is associated with poor prognosis, advanced TNM stage, and high TP53 mutation frequency. Besides, the m6Asig-Score was negatively associated with immune checkpoint inhibitors and anti-angiogenic drug response. We further found that two of the five m6Asig-Score inner genes, B2M and SMOX, were associated with immune cell infiltration, immune response, and the sensitivity to sorafenib, which were validated in two independent immunotherapy cohorts and the Genomics of Drug Sensitivity in Cancer (GDSC) database.Conclusion: We constructed a novel prognosis signature and identified B2M and SMOX for predicting immune and anti-angiogenic efficacy in HCC, which may guide the combined treatment strategies of immunotherapy and anti-angiogenic therapy in HCC.