bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cluster formation between an oxadiazole derivative with metal nanoclusters (Ag/Au/Cu), graphene quantum dot sheets, SERS studies and solvent effects
Authors: Al-Otaibi Jamelah S , Mary Y.Sheena , Mary Y.Shyma , Trivedi Ravi , Chakrabory Brahmananda , Thomas Renjith 
Published: April 2022

Abstract
Interaction of an oxadiazole derivative with Ag/Au/Cu and graphene quantum dots with different solvents is reported theoretically. The adsorption energy is maximum for Cu6 cluster and minimum for Ag6 cluster. The asymmetric charge redistribution between DPMO and M6s produces an improvement in dipole moment values. The decrease in energy gaps of complexes increased conductivity and metal clusters can be used as a drug sensor. The solvation energies are higher negative in solvent media than in the gaseous media, indicating an enhancement in solvent medium’s stability. Wavefunction studies show that there exist significant noncovalent interactions between metal clusters and oxadiazole that facilitates cluster formation. DPMO is found to form stable clusters with graphene which is evident from the enhancement of Raman activity of the system through SERS also enabling it for sensing DPMO in a mixture.
Etiologic, microbiologic, clinical and outcome characteristics of febrile neutropenia in children with malignancy
Authors: Solgun Huseyin Avni , Turk Zeynep Gunal , Bayram Cengiz , Aycicek Ali 
Published: April 2022

Abstract
Background
The purpose of this study is to examine the clinical, laboratory findings and treatments of pediatric patients who were followed up in our clinical institue for malignancy and those who had febrile neutropenia (FEN) attacks.
Material and Methods
55 patients between the ages of 1–17 and 83 episodes of FEN who were followed up and treated for cancer in our Pedaitric Hematology- Oncology clinic between January 2019 and June 2019 were examined cross-sectionally. Patients with absolute neutrophil count (ANC) < 500 / mm³ were included in the study. Febrile neutropenia was diagnosed in neutropenic patients with a single measurement of body temperature ≥ 38.3˚C (101˚F) or ≥ 38.0˚C (100.4˚F) for a period of 1 hour. The demographic information of the patients, laboratory and physical examination findings, FEN risk groups, the most recent chemotherapy protocols, fever and neutropenia durations, fever foci, culture reproduction, and treatments started were recorded in a standard form.
Results
Among 83 FEN attacks included in the study, 28 of the patients were female (34%) and 55 were male (66%). The median age was 5.5 (range 1.1–16.7) years. Sixty-six (80%) of the patients were being followed up for leukemia and 17 (20%) for solid tumors. The average number of attacks per patient was 1.4 (1–4) attacks. According to the risk classification of febrile neutropenia, 80 attacks (96%) were classified as high risk, and 3 attacks (4%) as low risk. Mean duration of fever was in patients with leukemia and in patients with solid tumors. Mean duration of neutropenia was significantly higher versus 1.9 ± 1.3 days to 1.9 ± 1.1 days in patients with leukemia than those treated for solid tumors (p˂0.05). The most common microorganism was Coagulase negative staphylococcus. Galactomannan was positive in one patient and Enterobacter growth was detected in the blood culture.
Conclusion
Febrile neutropenia is among the important causes of mortality in cancer patients. Most of the attacks in our study were high-risk FEN attacks. The reason for this may be duration of active chemotherapy, prelonged neutropenia periods, remission status of cancer, and delays related to hospitalization of patients. Fever focus is often not detected in FEN attacks, the most common fever focus in our study was mucositis. The most common gram-positive microorganisms were found in the blood culture of our patients. This study is important in terms of creating up-to-date algorithms in the treatment and management in FEN patients. However, the results to be obtained by conducting studies with more patients will be more beneficial in this sense.
Clinical characteristics and laboratory data of pre-eclampsia patients with and without in vitro fertilization embryo transfer treatment
Authors: Li Liman , Liu Yuan , Zhou Weijie , Jiang Shiling , Liu Ying , Feng Ting , Zhou Rong , Li Hong 
Published: April 2022

Abstract
BackgroundPre-eclampsia affects around 2-10% pregnancies in the world. In vitro fertilization embryo transfer (IVF) pregnancies are associated with a 2.7-fold risk of pre-eclampsia. MethodsIn our retrospective study, 20 pre-eclampsia patients with IVF treatment (PE with IVF group) and 20 matched pre-eclampsia controls with spontaneous conception (PE group) were enrolled. We also collected several fresh decidua tissue and cord blood specimens to determine functional changes of innate immune cells at maternal-fetal interface using flow cytometry assays. ResultsThere were no obvious differences in pregnancy outcomes between 2 groups. However, maternal prealbumin (PA) level was significantly decreased while creatinine (Cr) and uric acid (UA) levels were significantly elevated in PE with IVF group. Although levels of maternal alkaline phosphatase (ALP) and cystatin C (CysC) did not differ between 2 groups, they were both abnormally elevated, exceeding the normal reference range. Moreover, significant elevations of IFN-γ and TNF-α secretions were observed in both decidual NK cells and γδT cells of pre-eclampsia patients with IVF treatment. In cord blood, productions of IFN-γ and TNF-α were only increased in the NK cells of pre-eclampsia patients with IVF treatment. ConclusionThe close surveillance for clinical features of this special pre-eclampsia population is strongly warranted.
Bioinformatic analysis of SERINC2 with the prognosis and immune infiltration of stomach adenocarcinomas
Authors: Yu Haixia , Qiaoyun Ling , Chi Zhai , Fang Xianjun 
Published: April 2022

Abstract
Serine Incorporator 2 (SERINC2) is a member of SERINC family, which can incorporate serine into membrane lipids and contributes to the biosynthesis of membrane lipids. SERINC2 has been reported to be associated with tumor progression. However, the expression and function of SERINC2 in stomach adenocarcinomas (STAD) is still unclear. TIMER and GEPIA Database analysis confirmed the high expression of SERINC2 in STAD. Kaplan-Meier Plotter Database analysis showed that STAD patients with high expression level of SERINC2 had low post-progression survival, progression free survival and overall survival. However, gene alterations of SERINC2 have no significant influence on the prognosis of STAD. Moreover, we found that SERINC2 expression was negatively correlated with infiltrating levels of CD4+ and CD8+ T cells, macrophages, neutrophils and dendritic cells in STAD via TIMER. Further analysis from the LinkedOmics database suggested that the co-expressed genes of SERINC2 were enriched in the metabolic process and immune response. Three hub genes, S100A14, RAB25 and FXYD3, were identified through protein-protein network. Among the hub genes, RAB25 was also highly expressed and negatively associated with the survival of STAD patients. These results suggest that SERINC2 is highly expressed in STAD, leading to a poor prognosis and low infiltrating levels of immune cells. The function of SERINC in STAD may be associated with metabolic regulation and RAB25. Therefore, we suggest that it can be an effective biomarker for the diagnosis of STAD.
NDRG2 inhibits glycolysis in liver cancer cells is accompanied by the regulation of SIRT1
Authors: Xin Benge , cao wei 
Published: April 2022

Abstract
NDRG2 is a tumor suppressor, highly expressed in normal tissues but down-regulated in many cancers. This enzyme was involved in glycolysis by regulating glycolytic enzymes in ccRCC and colorectal cancer. However, the mechanism of NDRG2 regulating glycolysis is still not clear, and the function of NDRG2 in liver tumor glycolysis is completely unknown. In the present study, we first demonstrated the low expression of NDRG2 in liver tumor tissues and found that the survival rate of patients was negatively correlated with the expression of NDRG2. Subsequently, based on NDRG2 overexpressed and knockdown cell lines, NDRG2 was shown to have the ability to inhibit glycolysis in liver cancer cells. Finally, SIRT1, a deacetylase that plays important role in glycolysis regulation, is negatively regulated by NDRG2 in liver tumors. Our data enrich the understanding of the role of NDRG2 in tumor growth and improve the mechanism by which NDRG2 regulates glycolysis.
Comparison of short-term outcomes of single-incision versus multi-port laparoscopic surgery for descending colon cancer: a propensity score-matched analysis
Authors: Tei Mitsuyoshi , Suzuki Yozo , Sueda Toshinori , Iwamoto Kazuya , Naito Atsushi , Nomura Masatoshi , Yoshikawa Yukihiro , Ohtsuka Masahisa , Imasato Mitsunobu , Mizushima Tsunekazu , Akamatsu Hiroki 
Published: April 2022

Abstract
Purpose: Single-incision laparoscopic surgery (SILS) for descending colon cancer (DCC) is a technically challenging procedure with unclear clinical impact. The aim of this study was to evaluate the short-term outcomes of SILS for DCC compared with multi-port laparoscopic surgery (MPLS).Methods: We retrospectively analyzed 137 consecutive patients with stage I–III DCC who underwent SILS or MPLS at two high-volume multidisciplinary tertiary hospitals between April 2008 and December 2018, using propensity score-matched analysis.Results: After propensity score-matching, we enrolled 88 patients (n=44 in each group). Median follow-up period was 48 months. The completion rate for SILS was 98.1% in the overall cohort and 97.7% in the matched cohort. One patient was converted to open surgery in the MPLS group. Compared with the MPLS group, the SILS group showed significantly less blood loss and a greater number of harvested lymph nodes in both the entire patient cohort and the matched cohort. Morbidity rates did not differ significantly between groups before and after matching. R0 resection was achieved in the entire patient cohort. In terms of the incidence of recurrence, both groups were similar before and after matching. No significant differences were found between groups in terms of 3-year disease-free and overall survival rates in either the entire patient cohort or the matched cohort.Conclusions: SILS appears safe and feasible and can provide satisfactory oncological outcomes for selected patients with DCC.
Biallelic Mismatch Repair Deficiency – a rare and trouble genetic syndrome
Authors: Ramos Diana , Brandão Catarina , Sousa Cátia , Ribeiro Mário Dinis 
Published: April 2022

Abstract
Biallelic Mismatch Repair Deficiency (BMMRD) is a rare autosomal recessive disorder characterized by numerous cancers presenting as early as the first decade of life. Biallelic germline mutations in one of four mismatch repair (MMR) genes (MLH1, MSH2, MSH6, or PMS2) cause this devastating disease. Given the rarity of the syndrome, often-asymptomatic tumors and overlap with neurofibromatosis-1, diagnosis is frequently unrecognized or delayed. A high degree of clinical awareness is needed to identify new cases. Immunohistochemical assessment of MMR protein expression and analysis of microsatellite instability are the first tools with which to initiate the study of this syndrome in solid malignancies. MMR immunohistochemical shows a hallmark pattern with absence of staining in both neoplastic and non-neoplastic cells for the biallelic mutated gene.We present a unique case of a young boy diagnosed with invasive colon adenocarcinoma and brain tumor, with classical BMMRD features, found to have biallelic pathogenic PMS2 mutations.
Identification of a hub gene in hepatocellular carcinoma induced by nonalcoholic fatty liver disease using bioinformatics analysis
Authors: Li Juan , Wu Chutian , Liu Xiongxiu , Long Linjing , Zhong Lixian , Lai Junlong , Lin Junqin , Li Yuting , Zeng Qiuting , Tang Shaohui 
Published: April 2022

Abstract
Background: Hepatocellular carcinoma (HCC) is the fourth most common cause of cancer mortality worldwide and nonalcoholic fatty liver disease (NAFLD)-related HCC is related to poorer survival than viral hepatitis-related HCC. However, the pathogenetic process of NAFLD to HCC is still poorly understood. Material and methods: A series of bioinformatics analyses were performed in two public datasets (GSE164760, GSE37031), The Cancer Genome Atlas (TCGA) database, and the International Cancer Genome Consortium (ICGC) database to explore potential hub genes in NAFLD-related HCC. Results: Weighted Gene Co-Expression Network Analysis (WGCNA) and LASSO logistic regression were performed to filter out 8 hub genes, then TMEM126A was selected for further analysis for its better diagnostic, stratification, and prognostic value in HCC. The results of Gene Set Enrichment Analysis (GSEA) indicated that TMEM126A was highly enriched in lipid metabolism. Next, TMEM126A was significantly correlated with the fatty acid biosynthesis and oxidation pathways. Further validation in other datasets confirmed our results. Conclusion: TMEM126A is firstly screened out for a hub gene in the pathogenesis of NAFLD-related HCC. Although further experiments are supposed to verify these results, we provide useful and novel information to explore the underlying mechanism in NAFLD-related HCC.
Maternal hemodynamics during labor epidural analgesia with and without adrenaline: a secondary analysis of a randomized trial
Authors: Haidl Felix , Tronstad Christian , Rosseland Leiv Arne , Dahl Vegard 
Published: April 2022

Abstract
Background: Pregnancy in general and labor in particular is associated with changes in maternal hemodynamic parameters such as increased cardiac output and heart rate, with peaks during uterine contractions. Adrenaline may be added to labor epidural solutions to enhance efficiency, but the hemodynamic fluctuations may increase. The aim of this study was to compare the hemodynamic changes of epidural drug solution with or without adrenaline 2 µg.ml-1and to provide pilot data for a larger study.Methods:Forty-one nulliparous laboring women requesting epidural analgesia were randomized to epidural solution of bupivacaine 1mg.ml-1, fentanyl 2 µg.ml-1 with or without adrenaline 2 µg.ml-1. The participants were monitored with the Nexfin CC continuous non-invasive blood pressure and cardiac output monitor. The primary outcomes were changes in peak systolic blood pressure and cardiac output at uterine contraction within 30 minutes after epidural activation. The effect of adrenaline was tested statistically by a linear mixed effects model of the outcome variables’ dependency on time, adrenaline and their interaction. Results:The addition of adrenaline to the solution had no statistically significant effect on the temporal changes in peak systolic blood pressure (mean change 0.23 mmHg.min-1 95% CI [-0.17; 0.64] p=0.26), peak cardiac output (mean change 0.0029 l.min-1.min-1 95 % CI [-0.026; 0.032] 0.84), or heart rate (mean change 0.015 beats.min-1 .min-1 95 % CI [-0.25; 0.28] p=0.91). Conclusion:The addition of adrenaline 2 µg.ml-1to the epidural solution does not seem to have clinically significant influences on the maternal hemodynamics during labor.Trial registration:This trial was registered at clinicaltrials.gov (NCT00685672) May 28th 2008.
Expression of the Fab enzymes (Fab I and Fab Z) from Plasmodium falciparum after exposure to Artemisia afra plant extracts and drugs screening
Authors: Kane Ndeye Fatou , Kyama Mutinda Cleophas , Nganga Joseph Kangethe , Hassanali Ahmed , Diallo Mouhamadou , Kimani Francis 
Published: April 2022

Abstract
The emergence and spread of drug resistance of the malaria parasite to the main treatment emphasize the need to develop new antimalarial drugs. In this context, the fatty acid biosynthesis (FAS_II) pathway of the malaria parasite is one of the ideal targets due to its crucial role in parasite survival. In this study, we report the expression and the affinity binding of Fab_I and Fab_Z after exposure of the parasite with different extracts of the Artemisia afra. The parasites were exposed for 2 days to different extracts. Gene expression was done to determine the level of expression of the fab enzymes after treatments. A GCMS was run to determine the different compounds of the plant extracts followed by a virtual screening between the fab enzymes and the active compounds using Pyrex. The results showed different expression patterns of the Fab enzymes. Fab_I was downregulated in the W2 and D6 strains by the ethanolic extract but was increased by Hexane and DCM extracts. A different expression pattern was observed for Fab_Z. it was all upregulated except in the D6 strain when exposed to the ethanolic and hexane extracts. Virtual screening showed affinity with many compounds. Hits compounds with high binding energy were detected. 11alphaHydroxyprogesterone and Aspidospermidin-17-ol were found to have high binding energy with Fab_I respectively (-10.7 kcal/mol; -10.2kcal/mol). Fab_Z shows also high affinity with 11alpha-Hydroxyprogesterone (-10kcal/mol) and Thiourea (-8.4 Kcal/mol). These results demonstrate the potential of A. afra as a big source of new antimalarial drugs.
Mathematical model for predicting the usage of the transmission ratio of a wheel skidder
Authors: Михайлов О.А. , Андронов А.В. , Тарадин Г.С. , Козьмин С.Ф. , Зверев И.А. 
Published: April 2022

Трелевочный трактор является одним из важнейших видов лесосечной техники, требующей грамотного подхода к процессу проектирования, который учитывает особенности условий эксплуатации данного вида техники. Одной из самых нагруженных частей колесных скиддеров является трансмиссия, которая испытывает значительные нагрузки в процессе эксплуатации. Для регистрации и измерения значений величин этих нагрузок в зависимости от передачи, на которой осуществляется процесс трелевки пачки деревьев, проведены испытания трелевочного трактора ТЛК-1, проходившие на полигоне-волоке Онежского тракторного завода. В результате испытаний определены значения крутящих моментов на полуосях и карданном валу скиддера на различных передачах, а также время использования каждой из передач. Полученные в ходе испытаний данные обработаны с помощью методов стохастического математического моделирования. В результате получена математическая модель прогнозирования использования передаточного числа трансмиссии колесного трелевочного трактора, оснащенного гидродинамической передачей. Данная методика позволяет на стадии проектирования оценить правильность выбора передаточного ряда трансмиссии.
The skidder is one of the most important types of forestry it requires a competent approach to the design process, which of this type of equipment is one of the most important types of forestry machinery. One of the most loaded part of wheeled skidders is the transmission, which it experiences significant loads in the process of operation. To record and to measure the values of these loads depending on the gear, on which the process of skidding a pack of trees is carried out. Which the process of skidding a pack of trees is carried out, were carried out tests of the skidder TLK-1, which took place on the firing ground Onega tractor plant. As a result of tests were determined values of torques on axles and cardan shaft of the skidder on different gears, as well as the time of using each of the gears. The data obtained during the tests were processed using the methods of stochastic mathematical modeling. As a result, a mathematical model mathematical model to predict the usage of gear ratio of a wheeled skidder transmission equipped with hydrodynamic transmission. This methodology allows to estimate the correctness of the choice of the transmission ratio at the design stage. Design stage to assess the correctness of the choice of the transmission gear ratio.
Soil and vegetation complex of the «Dubki» Park in St. Petersburg, Russia
Authors: Ковязин В.Ф. , Иванова Е.А. 
Published: April 2022

Исследование посвящено изучению почвенно-растительного комплекса памятника ландшафтной архитектуры XVIII века – парка «Дубки», расположенного на северном побережье Финского залива в Курортном районе Санкт-Петербурга, городе Сестрорецке. Парк создан по инициативе императора Петра I, который во время возвращения с Гангутской битвы сделал остановку среди дубовой рощи на берегу Финского залива. В настоящее время парк признан памятником культурно-исторического наследия, охраняется ЮНЕСКО. Летом 2021 г. на объекте изучены морфология почвы, видовое разнообразие хвойных, лиственных пород и кустарников. Морфология почвы изучалась по двум почвенным разрезам, заложенным в различных лесорастительных условиях. Видовой состав насаждений парков определялся по определителю растений [Киселева и др., 2020], с использованием морфологических признаков листьев и коры. С применением программных комплексов РГИС и AutoCAD рассчитаны площади земельных угодий парка под растительностью, под дорожками, искусственными водоемами, спортивными площадками и общая.
The research is devoted to the study of the soil and plant complex of the monument of landscape architecture of the XVIII century – the park «Dubki» in St. Petersburg, located on the northern coast of the Gulf of Finland in the Resort area of St. Petersburg, in the city of Sestroretsk. The park was created on the initiative of Emperor Peter the Great, who, during his return from the Battle of Gangut, made a stop among an oak grove on the shore of the Gulf of Finland. Currently, the park is recognized as a monument of cultural and historical heritage, protected by UNESCO. In the summer of 2021, the morphology of the soil, the species diversity of coniferous, deciduous species and shrubs were studied at the facility. The morphology of the soil was studied by 2 soil sections laid down in various forest growing conditions. The species composition of park plantings was determined by the plant determinant [Kiseleva et al., 2020], using morphological features of leaves and bark. With the use of the RGIS and AutoCAD software complexes, the areas of the park's land under vegetation, under paths, artificial reservoirs, sports grounds, etc. are calculated. Based on the results of the research, recommendations are given for maintaining the balance and sustainable development of the soil and plant complex and information on the development of the land cadastre of the park.
Fermented oat milk as a base for lactose-free sauce
Authors: Khrundin Dmitrii , Ponomarev Vsevolod , Yunusov Eduard 
Published: April 2022

Introduction. We studied the use of fermented oat milk to produce sauce and evaluated its properties. The research was motivated by the current demand for so called “plant milk” commonly perceived as an alternative to cow’s milk.
Study objects and methods. The experimental samples were produced from oats-based drinks (1.5 and 3.2% fat) fermented with starter cultures of lactic acid microorganisms following the guidelines for yoghurt production. Apple pectin was used as a thickener. Rheological studies were performed using an RM-1 rotational viscometer and a CT-2 texture analyzer according to the standard methods. Sensory evaluation was based on a scoring scale. Physicochemical parameters were determined according to generally accepted methods.
Results and discussion. Oat milk was fermented to produce a sauce base. Acid accumulation increased throughout fermentation up to 135–137°T. Apple pectin (3%) was added to stabilize the structure and ensure the desired consistency. Higher concentrations of pectin increased the hardness and adhesive strength of the samples from both 1.5 and 3.2% oat milk. The 1.5% sauce scored highest in the sensory evaluation. Its physicochemical indicators met the standard requirements for related fermented milk products. We found the best consistency indicators at a pectin concentration of 3%.
Conclusion. The new fermented sauce based on low fat oat milk (1.5% fat) had high consumer appeal as well as physicochemical, sensory, and rheological characteristics. The sauce can be used by people with lactose intolerance and vegetarians.