bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extraperitoneal tissue retraction technique: An effective assistant of extraperitoneal pure single-port robotic-assisted radical prostatectomy with the da Vinci Si surgical system
Authors: Ju Guanqun , Wang Zhijun , Shi Jiazi , Xu Weidong , Zhang Zongqin , Yin Lei , Xu Dongliang , Ren Shancheng 
Published: October 2022

ObjectiveThe limitations of tissue retraction and the amount of surgical working space have a great impact on extraperitoneal single-port robotic-assisted radical prostatectomy (sp-RARP) with the multiport robotic surgical system. We used an extraperitoneal tissue retraction technique to achieve tissue exposure and working space expansion. This study evaluated the safety, feasibility, and efficacy of the extraperitoneal tissue retraction technique in extraperitoneal pure sp-RARP with the da Vinci Si surgical system.MethodsData from 42 patients were analyzed retrospectively from December 2018 to February 2020. The extraperitoneal tissue retraction technique was not used in 20 patients (group I) and was used in 22 patients (group II). Preoperative, intraoperative, and postoperative data were collected. The oncological and functional data during late follow-up were recorded.ResultsAll patients successfully underwent extraperitoneal pure sp-RARP. No patients required conversion to a multiport surgery or placement of additional assistant ports. The two groups were similar regarding baseline features. The median operation time in group I was significantly longer than that in group II (P &lt; 0.001). The estimated blood loss volume in group I was significantly higher than that in group II (P &lt; 0.001). There were no serious complications in either group. There were four cases of peritoneal tears in group I and none in group II (P = 0.043). The surgical margin and lymph nodes were negative in both groups. The oncological and functional outcomes were similar between the two groups 6 months after the procedure.ConclusionsThe extraperitoneal tissue retraction technique is safe and feasible. The technique promotes tissue exposure and expands the surgical working space, which is important for achieving extraperitoneal pure sp-RARP with the da Vinci Si surgical system, especially for beginners. The short-term oncological and functional outcomes were within acceptable ranges. The long-term effects of this technique need further evaluation.
The Impact of the COVID-19 Pandemic on Tuberculosis Case Notification and Treatment Outcomes in Eswatini
Authors: Masina Hloniphile Victory , Lin I-Feng , Chien Li-Yin 
Published: October 2022

Objectives: We investigated the impact of COVID-19 on tuberculosis (TB) case notification and treatment outcomes in Eswatini.Methods: A comparative retrospective cohort study was conducted using TB data from eight facilities. An interrupted time series analysis, using segmented Poisson regression was done to assess the impact of COVID-19 on TB case notification comparing period before (December 2018-February 2020, n = 1,560) and during the pandemic (March 2020–May 2021, n = 840). Case notification was defined as number of TB cases registered in the TB treatment register. Treatment outcomes was result assigned to patients at the end of treatment according to WHO rules.Results: There was a significant decrease in TB case notification (IRR 0.71, 95% CI: 0.60–0.83) and a significant increase in death rate among registrants during the pandemic (21.3%) compared to pre-pandemic (10.8%, p &lt; 0.01). Logistic regression indicated higher odds of unfavorable outcomes (death, lost-to-follow-up, and not evaluated) during the pandemic than pre-pandemic (aOR 2.91, 95% CI: 2.17–3.89).Conclusion: COVID-19 negatively impacted TB services in Eswatini. Eswatini should invest in strategies to safe-guard the health system against similar pandemics.
Semicarbazide-Sensitive Amine Oxidase (SSAO) and Lysyl Oxidase (LOX) Association in Rat Aortic Vascular Smooth Muscle Cells
Authors: Manasieva Vesna , Thakur Shori , Lione Lisa A. , Patel Jessal , Baydoun Anwar , Skamarauskas John 
Published: October 2022

Vascular smooth muscle cells (VSMCs) are the main stromal cells in the medial layer of the vascular wall. These cells produce the extracellular matrix (ECM) and are involved in many pathological changes in the vascular wall. Semicarbazide-sensitive amine oxidase (SSAO) and lysyl oxidase (LOX) are vascular enzymes associated with the development of atherosclerosis. In the vascular smooth muscle cells, increased SSAO activity elevates reactive oxygen species (ROS) and induces VSMCs death; increased LOX induces chemotaxis through hydrogen peroxide dependent mechanisms; and decreased LOX contributes to endothelial dysfunction. This study investigates the relationship between SSAO and LOX in VSMCs by studying their activity, protein, and mRNA levels during VSMCs passaging and after silencing the LOX gene, while using their respective substrates and inhibitors. At the basal level, LOX activity decreased with passage and its protein expression was maintained between passages. βAPN abolished LOX activity (** p < 0.01 for 8 vs. 3 and * p < 0.05 for 5 vs. 8) and had no effect on LOX protein and mRNA levels. MDL72527 reduced LOX activity at passage 3 and 5 (## p < 0.01) and had no effect on LOX protein, and mRNA expression. At the basal level, SSAO activity also decreased with passage, and its protein expression was maintained between passages. MDL72527 abolished SSAO activity (**** p < 0.0001 for 8 vs. 3 and * p < 0.05 for 5 vs. 8), VAP-1 expression at passage 5 (** p < 0.01) and 8 (**** p < 0.0001), and Aoc3 mRNA levels at passage 8 (* p < 0.05). βAPN inhibited SSAO activity (**** p < 0.0001 for 5 vs. 3 and 8 vs. 3 and * p < 0.05 for 5 vs. 8), VAP-1 expression at passage 3 (* p < 0.05), and Aoc3 mRNA levels at passage 3 (* p < 0.05). Knockdown of the LOX gene (**** p < 0.0001 for Si6 vs. Sictrl and *** p < 0.001 for Si8 vs. Sictrl) and LOX protein (** p < 0.01 for Si6 and Si8 vs. Sictrl) in VSMCs at passage 3 resulted in a reduction in Aoc3 mRNA (#### p < 0.0001 for Si6 vs. Sictrl and ### p < 0.001 for Si8 vs. Sictrl) and VAP-1 protein (# p < 0.05 for Si8 vs. Sictrl). These novel findings demonstrate a passage dependent decrease in LOX activity and increase in SSAO activity in rat aortic VSMCs and show an association between both enzymes in early passage rat aortic VSMCs, where LOX was identified as a regulator of SSAO activity, protein, and mRNA expression.
AVALIAÇÃO DA RESISTÊNCIA AO MANCHAMENTO DE RESINAS COMPOSTAS EXPOSTAS À SUBSTÂNCIA CORANTE
Authors: Quoos Macedo Karen Camila , Borges Tássia Silvana , Oliveira Danilo Flamini 
Published: October 2022

A utilização de resinas compostas no dia a dia clínico vem ganhando cada vez mais espaço, principalmente com a grande popularidade das facetas em resinas composta. Esses materiais necessitam possuir propriedades básicas para utilização, a exemplo: estabilidade de cor, insolubilidade, biocompatibilidade. Estes compósitos podem sofrer alterações de cor ao longo do tempo, por fatores intrínsecos ou extrínsecos. Dentre os fatores extrínsecos, a absorção de pigmentos da alimentação. O objetivo deste estudo foi verificar a suscetibilidade ao machamento de três marcas distintas de resina composta frente ao açafrão, corante amplamente utilizado na região Norte. Para o desenvolvimento da metodologia foram utilizados 60 corpos de prova, sendo 20 de cada resina selecionada. Para cada resina, 10 corpos de prova foram imersos em água destilada e os outros 10 em solução contendo açafrão (n=10). As resinas selecionadas para obtenção dos corpos de prova foram a Opallis (FGM); Charisma Classic (Kulzer); Filtek Z350 (3M ESPE). As leituras iniciais de cor realizaram-se em todos os corpos de prova imediatamente após tratamento dos corpos e imersão em água destilada por 24 horas. A leitura subsequente se deu após 15 dias para cada resina submetida à imersão em solução de açafrão. As variações de coloração foram novamente levadas para mensuração por meio de aparelho espectrofotrômetro, porém não foi possível devido alto manchamento e consistência de cor fora do padrão. Dessa maneira, os resultados foram comparados por meio da fotografia, que mostrou que a estabilidade de cor das resinas estudadas sofreu influência do corante utilizado. Todas as resinas sofreram pigmentação de maneira equivalente de acordo com a sua cor inicial.
THE MENTAL ORCHESTRATES THE INFORMATION ACTIVITY OF THE BRAIN: FROM PHYSICAL EXISTENCE TO MENTALLY REALIZED BEING
Authors: Soloviov O. , Litvinova O. 
Published: October 2022

The article is devoted to the question of the functional specificity of the mental (psyche, subjective) during the informational activity of neural networks of the brain, which have a physiological (by and large, physical) nature. The understanding of such specificity of the mental is based on the awareness of the inability of physical laws to support the biological and/or social expediency of the brain's informational activity, which it actually carries out. It is also shown that such an idea of the mental, taking into account the features that are revealed here due to its functional and, hence, causal inclusion in the processing of information in the brain, should change the conceptual foundations of psychology and make its practical applications more understandable.
It was found that the key aspect of psychic activity is the ability of living beings to evaluate anything subjectively, that is, qualitatively (not quantitatively, like a computer). Therefore, subjective assessment is interpreted as an information processing operator in the brain, which ensures the integration of information for the purpose of creating biologically/socially appropriate mental models of the future relations of a living being with the surrounding reality. These mentally active models of the future (goals, plans, ideas) "orchestrate" (determine) the motor acts of a living being based on past experience integrated with subjective assessments in order to change the "world for the better". The functional specificity of the human psyche is also revealed, for which two new terms are introduced, namely, mono-valued psyche and multi-valued psyche. This makes it possible to describe the "mechanism" of human freedom, which unfolds, "contrary" to classical physical ideas, on the background of physical laws and through the activity of the brain. The introduced concepts make it possible to characterize a mentally active person as a living being that most effectively demonstrates causality-from-within as a factor of informational resistance to physical entropy.
COPING STRATEGIES AND MAINTAINING PRODUCTIVITY IN STRESSFUL SITUATIONS
Authors: Kuzikova S. , Shcherbak T. 
Published: October 2022

The article is devoted to the analysis of the results of theoretical and empirical research of students’ coping strategies and the connection of coping with the preservation of productivity and high functionality in stressful situations (in relation to the results of diagnosis of students’ procrastination). Theoretical analysis of the specifics of the effectiveness of coping strategies is presented. The empirical study involved 66 students of 2-4 courses. Diagnosis of coping tension was performed using the Ways of Coping Questionnaire, WCQ (R. Lazarus) and "Tuckman General Procrastination Scale (adapted by T. L. Kryukova)". The results of an empirical study of students' coping have been analyzed. It has been found that the most often behavioral strategies to which students apply are: planning (57.5%) and positive reassessment (56.6%); the search for social support, acceptance of responsibility and self-control are equally pronounced (31.8%); distancing, avoidance, and confrontational coping are less common. The severity and dominance of coping strategies in groups of respondents with different levels of procrastination have been analyzed. According to the results, the most developed behavioral strategies for students with low procrastination are positive reassessment and planning (the latter is more often represented by the dominant strategy). Among the group with a medium level of procrastination, these two copings are also equally pronounced. However, there is a lack of tension in other, active behavioral strategies. Among people with a high level of procrastination, the statistical predominance of one or another coping is not recorded, we widely observe the severity of only one or two strategies, or low tension of all presented copings. After analyzing the dominant copings of people with low procrastination, a hypothesis was formulated about the possible inverse correlation between the severity of positive revaluation and procrastination, the test was performed by mathematical data processing (Spearman's correlation analysis). No significant correlations were found, so the hypothesis was not confirmed. The results of empirical research can be used both to develop recommendations, developmental and educational activities aimed at preventing the decline in productivity and efficiency, and to highlight the understudied topic of the links between coping strategies and the phenomenon of procrastination.
FEATURES OF COPING STRATEGIES IN MEN AND WOMEN DURING THE MARTIAL LAW
Authors: Myshko N. , Titova T. , Teslenko M. , Udina N. 
Published: October 2022

The article discusses the issue of coping behavior as a form of behavior that reflects an individual's readiness to solve life problems. What is especially relevant in times of trials from military threats in the country. This behavior is aimed at adapting to circumstances and involves the developed ability to use certain means to overcome emotional stress. When choosing active actions, the probability of eliminating the effect of stressors on a person increases. The theoretical analysis showed that coping strategies are necessary when a person gets into a crisis situation. Any crisis situation presupposes the presence of some objective circumstances and a certain attitude of a person to them, depending on the degree of their significance, which is accompanied by emotional and behavioral reactions of various nature and degree of intensity. The leading characteristics of a crisis situation are mental tension, significant experiences as a special internal work to overcome life events or traumas, a change in self-esteem and motivation, as well as a pronounced need for their correction and psychological support from the outside.
It was found that the motivational orientation of the individual determines the features of coping strategies in crisis situations. Thus, subjects with a pronounced motivation to avoid failure are more likely to use passive behavioral strategies in stressful situations - social support and avoidance, which reflects their weak efforts in solving any problem situation. And the subjects with a high level of motivation for success mainly use the strategy of finding a solution in stressful situations, aimed at solving the problem and adequately responding to the stressful situation. It was established that almost half of the researched use the strategy of finding a solution in stressful situations. That is, they have the highest level of development of adaptive coping mechanisms, tend to analyze stressful situations and find ways to solve them, have high confidence in their own resources to overcome stressful situations. The application of practical steps aimed at solving the situation significantly increases the constructiveness and effectiveness of overcoming it.
PSYCHOLOGICAL SPACE AND PERSONAL BOUNDARIES FORMATION AS A FACTOR OF STUDENTS’ PSYCHOLOGICAL WELL-BEING
Authors: Volynchuk O. 
Published: October 2022

The article presents the results of the study of students' psychological space, in particular the levels of sovereignty of personal space in terms of body, territory, things, habits, social ties and values, as well as signs of psychological boundaries: characteristics of their functions and thickness. It has been found that the students’ sovereignty of values is most formed, the sovereignty of things is also at a high level, and the sovereignty of the body is the least formed, which may indicate physical deprivation or at least a tendency to it.
The psychological well-being of students is studied. It is found that its parameters are characterized by an average level for most respondents, while the overall rate of psychological well-being is low for 2/3 of respondents.
There are direct and inverse relationships between the parameters of psychological well-being and the characteristics of students’ personal boundaries and space. It is found that the indicators of the sovereignty of psychological space with the characteristics of well-being are interrelated only directly, that is, the formed sovereignty is a condition of well-being feeling, and deprivation of space is a factor of ill-being. Signs of psychological boundaries are also mostly positively related to the well-being. It’s established that only the impermissible and restraining functions of the boundary correlate negatively with the psychological well-being.
PROFESSIONAL SELF-IDENTITY OF FUTURE PSYCHOLOGISTS IN INSTITUTIONS OF HIGHER EDUCATION
Authors: Peretiatko L. , Teslenko M. 
Published: October 2022

The article analyzes theoretical and empirical approaches to the study of psychological prerequisites for the development of professional self-identity. The peculiarities of the future psychologists motivational sphere development as a key component of professional self-identity, the psychological and pedagogical conditions of its formation are theoretically investigated; principles of studying self-identity among psychologists are presented.
Methodological principles and diagnostic methods of researching the peculiarities of the professional self-identity of future psychologists and its factors are methodologically substantiated. The results of an empirical study, which characterize the peculiarities of the professional self-identity of psychology students at various educational stages of professional mastery, are analyzed.
Desafios do acesso à saúde bucal: uma revisão integrativa da literatura
Authors: Rabello Ricardo Elias Duarte , Monteiro Ângela Xavier , Lemos Sônia Maria , Teixeira Elizabeth , Honorato Eduardo Jorge Sant’Ana 
Published: October 2022

Quando focamos o acesso da população à saúde bucal na Atenção Primária à Saúde, ficamos diante de um grande desafio, que envolve garantir um serviço de qualidade, facilitado, integral e resolutivo. O objetivo deste estudo foi analisar a qualidade do acesso à saúde bucal na Atenção Primária à Saúde. Para isso, foi realizada, em outubro de 2020, uma revisão integrativa da literatura por meio de artigos publicados nos últimos cinco anos, disponíveis na Biblioteca Virtual da Saúde. A estratégia de busca retornou 118 artigos, desses 16 foram incluídos. As quatro categorias que emergiram da análise foram: Avaliação do acesso aos serviços odontológicos pela população e seus desafios; O impacto do acesso à saúde bucal na qualidade de vida das pessoas; Compreensão da relação do profissional com o acesso e O uso da agenda de atividades para a melhoria do acesso aos serviços de saúde bucal. Com essa análise, concluiu-se que ainda há muitos desafios a serem vencidos para que consigamos ter um acesso efetivo de qualidade à saúde bucal na APS.
Uma proposta metodológica para Análise do Discurso baseada na hermenêutica de Paul Ricoeur
Authors: Diniz Caetano da Providência Santos , Pimentel Adelma do Socorro Gonçalves 
Published: October 2022

Este estudo tem como objetivo apresentar uma proposta metodológica de análise do discurso baseada na fenomenologia hermenêutica de Paul Ricoeur. Discutiu-se seu conceito de interpretação e implicações para a análise do discurso. Não obstante Paul Ricoeur tenha enfatizado em a produção escrita, destacou-se as especificidades das narrativas orais produzidas nos contextos de pesquisa tendo por base as relações interpessoais entre colaboradores e pesquisadores e elaborou-se o instrumento “Fluxograma para Análise Fenomenológica do Discurso em Paul Ricoeur”, composto de quatro etapas, o recorte do texto, as marcas linguísticas, os sentidos vivenciais e a compreensão hermenêutica, com o objetivo de auxiliar o pesquisador na construção de sentidos tendo como base os discursos produzidos na interação com os participantes em pesquisas qualitativas.
A COL4A4-G394S Variant and Impaired Collagen IV Trimerization in a Patient with Mild Alport Syndrome
Authors: Kohler Jennefer , Omachi Kohei , Charu Vivek , Miner Jeffrey H. , Bhalla Vivek 
Published: October 2022

Key Points

Missense variants in COL4A genes can cause mild forms of Alport syndrome.Combining pathology and genetics with basic science can successfully determine the pathogenicity of variants of uncertain significance.Low-throughput, mechanistic approaches, applied broadly, may provide a critical next step for precision and personalized medicine.

Background
Missense variants in COL4A genes are often found in patients with an Alport syndrome–like presentation, but their pathogenicity is not always clear. We encountered a woman with microscopic hematuria and proteinuria at 33 years of age with a diagnosis of thin basement membrane disease who was approaching end stage kidney disease at 59 years of age. We hypothesized that this patient’s kidney disease was within the spectrum of Alport syndrome.

Methods
We used histologic, genetic, and biochemical approaches to investigate the mechanisms of kidney disease. By immunofluorescence, we investigated collagen IV chain composition of the glomerular basement membrane (GBM). We employed targeted sequencing to search for pathogenic variants in COL4A and other relevant genes. We utilized N- and C-terminal split NanoLuciferase assays to determine the effect of a novel COL4A4 variant of uncertain significance (VUS) on collagen IV heterotrimer formation in vitro. We transfected COL4A4 expression constructs with split NanoLuciferase fragment-fused COL4A3 and COL4A5 constructs into human embryonic kidney 293T cells. To assay for α3α4α5(IV) heterotrimer formation and secretion, we measured luminescence in cell lysates and culture supernatants from transfected cells.

Results
Immunostaining suggested that the collagen α3α4α5(IV) network was present throughout the patient’s GBMs. DNA sequencing revealed a novel homozygous VUS: COL4A4 c.1180G>A (p. Gly394Ser). In the C-terminal split luciferase-based α3α4α5(IV) heterotrimer formation assays, luminescence levels for G394S were comparable to WT, but in the N-terminal tag assays, the extracellular luminescence levels for G394S were decreased by approximately 50% compared with WT.

Conclusions
Our cell-based assay provides a platform to test COL4 VUS and shows that G394S impairs assembly of the α3α4α5(IV) N-terminus and subsequent trimer secretion. These data suggest that the COL4A4-G394S variant is pathogenic and causes an atypical mild form of autosomal recessive Alport syndrome.