bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of a high-fat meal on inflammatory and endothelial injury biomarkers in accordance with adiposity status: a cross-sectional study
Authors: de Souza Maria das Graças Coelho , Maranhão Priscila Alves , Panazzolo Diogo Guarnieri , Nogueira Neto José Firmino , Bouskela Eliete , Kraemer-Aguiar Luiz Guilherme 
Published: October 2022

Abstract
Background
It is known that consuming a high-fat meal (HFM) induces microvascular dysfunction (MD) in eutrophic women and aggravates it in those with obesity. Our purpose was to investigate if the MD observed after a single HFM intake is caused by endothelial damage or increased inflammatory state, both determined by blood biomarkers.

Methods
Nineteen women with obesity (BMI 30-34.9 kg/m2) and 18 eutrophic ones (BMI 20.0-24.9 kg/m2) were enrolled into two groups: Obese (OBG) and Control (CG), respectively. Blood samples were collected at five-time points: before (fasting state) and 30, 60, 120, and 180 min after HFM intake to determine levels of adipokines (adiponectin, leptin), non-esterified fatty acid (NEFA), inflammatory [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6)] and endothelium damage [soluble E-selectin, soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble intercellular adhesion molecule-1 (sICAM-1), plasminogen activator inhibitor-1 (PAI-1)] biomarkers.

Results
Levels of soluble E-selectin, leptin, and PAI-1 were higher in OBG at all-time points (P < 0.05) compared to CG. In the fasting state, OBG had higher levels of NEFA compared to CG (P < 0.05). In intra-group analysis, no significant change in the levels of circulating inflammatory and endothelial injury biomarkers was observed after HFM intake, independently of the group.

Conclusion
Our findings suggest that women with obesity have an increased pro-inflammatory state and more significant endothelial injury compared to eutrophic ones. However, the consumption of a HFM was not sufficient to change circulating levels of inflammatory and endothelial injury biomarkers in either group.

Registration number for clinical trials:
NCT01692327.
Effect of high-intensity interval training on aerobic capacity and fatigue among patients with prostate cancer: a meta-analysis
Authors: Chang Ming , Wang Junguo , Hashim Hairul A. , Xie Shihao , Malik Adam A. 
Published: October 2022

Abstract
Objective
This study focused on evaluating whether high-intensity interval training (HIIT) had an effect on aerobic capacity and fatigue among patients with prostate cancer (PCa) and exploring its effect on the immune system of PCa patients.

Methods
To investigate the potential effect of HIIT on patients with prostate cancer, a meta-analysis was carried out. From January 2012 to August 2022, studies that met predefined criteria were searched in the Scopus, PubMed, Web of Science, and EBSCO databases. Analysis of the standardized mean differences was performed using Review Manager 5.4.1 software with a 95% confidence interval.

Results
This review examined a total of 6 articles. There were 215 male patients with PCa involved, and the mean age was 64.4 years. According to the results of the meta-analysis, the HIIT group (n = 63) had greater VO2peak (P<0.01) than the control group (CON) (n = 52) (P = 0.30, I2 = 19% in the heterogeneity test; MD, 1.39 [0.50, 2.27]). Moreover, fatigue was significantly different (P<0.01) between the HIIT (n = 62) and CON (n = 61) groups (P = 0.78, I2 = 0% in the heterogeneity test; SMD, −0.52 [−0.88, −0.16]). Furthermore, among PCa patients, HIIT showed higher efficacy (P < 0.01) in decreasing PSA than the CON regimen (P=0.22, I2 = 34% in the heterogeneity test; MD, −1.13 [−1.91, −0.34]).

Conclusions
HIIT improves aerobic capacity, fatigue, and PSA levels among PCa patients but does not significantly affect IL-6 or TNF-α content. Therefore, HIIT may be a novel and potent intervention scheme for PCa patients.
First genetic report of Ixodes kashmiricus and associated Rickettsia sp.
Authors: Numan Muhammad , Islam Nabeela , Adnan Muhammad , Zaman Safi Sher , Chitimia-Dobler Lidia , Labruna Marcelo B. , Ali Abid 
Published: October 2022

Abstract
Background
Hard ticks (Ixodidae) are hematophagous ectoparasites that transmit various pathogens to a variety of hosts including humans. Transhumant herds have been involved in the spread of ticks and associated Rickettsia spp., and studies on this neglected topic have been unexplored in many regions including Pakistan. This study aimed to investigate ticks infesting transhumant herds of sheep (Ovis aries) and goats (Capra hircus) in district Shangla, Khyber Pakhtunkhwa, Pakistan.

Methods
Of the 144 examined animals, 112 hosts (68 sheep and 44 goats) of transhumant herds were infested by 419 ticks of different life stages including nymphs (105; 25%), males (58; 14%) and females (256; 61%). For molecular analyses, DNA was extracted from 64 collected ticks and subjected to PCR for the amplification of tick 16S rDNA and ITS2 partial sequences and for the amplification of rickettsial gltA and ompA gene sequences.

Results
All tick specimens were identified as Ixodes kashmiricus based on morphological features. The obtained 16S rDNA and ITS2 sequences showed 95.7% and 95.3% identity, respectively, with Ixodes kazakstani reported from Kyrgyzstan. In the phylogenetic tree, the sequences clustered with members of the Ixodes ricinus species complex, including I. kazakstani and Ixodes apronophorus. Additionally, rickettsial gltA and ompA partial sequences were 99.7% identical to Rickettsia sp. endosymbiont of Ixodes spp. from Panama and Costa Rica and 99.2% with Rickettsia endosymbiont from the USA. Phylogenetically, the rickettsial gltA and ompA partial sequences from I. kashmiricus clustered with various haplotypes of Rickettsia endosymbiont, which were sister cladded to Rickettsia monacensis.

Conclusions
This is the first genetic report of I. kashmiricus and associated Rickettsia sp. Large-scale tick surveillance studies across the country are needed to investigate Ixodes ticks and associated pathogens.

Graphical Abstract
Planning of distributed renewable energy systems under uncertainty based on statistical machine learning
Authors: Fu Xueqian , Wu Xianping , Zhang Chunyu , Fan Shaoqian , Liu Nian 
Published: October 2022

AbstractThe development of distributed renewable energy, such as photovoltaic power and wind power generation, makes the energy system cleaner, and is of great significance in reducing carbon emissions. However, weather can affect distributed renewable energy power generation, and the uncertainty of output brings challenges to uncertainty planning for distributed renewable energy. Energy systems with high penetration of distributed renewable energy involve the high-dimensional, nonlinear dynamics of large-scale complex systems, and the optimal solution of the uncertainty model is a difficult problem. From the perspective of statistical machine learning, the theory of planning of distributed renewable energy systems under uncertainty is reviewed and some key technologies are put forward for applying advanced artificial intelligence to distributed renewable power uncertainty planning.
Algerian extra hard cheese of Klila: a review on the production method, and microbial, organoleptic, and nutritional properties
Authors: Benamara Rym Nouria , Benahmed Meryem , Ibri Kada , Moussa Boudjemaa Boumediene , Demarigny Yann 
Published: October 2022

AbstractThis paper reviews the traditional practice of Klila production, its microbiological, physicochemical and nutritional properties and aims to raise awareness of Klila. The main objective of this review is to highlight the typicality of Klila and to propose a form of labelling that could help promote it. The Klila is a traditional extra hard cheese from southern Algeria. Nomadic tribes produce it from goat, sheep, or cow milk. Curdled milk is obtained by spontaneous fermentation. It is churned to recover the butter. The co-product, a sour, fat-free milk: Lben, undergoes a moderate heat treatment to get a fresh cheese which, after a dehydration process, gives the Klila. The chemical composition of this product varies regarding fat content. It has a very low water content (less than 10%) and comes in very hard pieces of varying size and irregular shape. The dry matter, for instance, can vary from 33 to 95%. Water availability (Aw) varies from 0.32 to 0.467. Fat rate can vary a lot from region to region from 9.5 to 29.3 g/100 g of cheese. The protein rate also varies a lot: between 29.9 and 71.4 g/100 g of cheese. The lactose concentration can range within 1.2 to 2.4 g/100 mL. The microbiological quality of Klila depends mainly on the quality of the raw milk used and all hygienic factors that surround milk production. The physicochemical conditions that prevail inside Klila are not favourable for the microbial growth. The Klila is an example of adaptation of the nomads’ lifestyle in the Algerian Sahara who use it as a culinary adjunct for nutritional and therapeutic purposes. The Klila can be grinded to be put as ingredient in “Zrizri” dessert with dates and clarified butter (smen). Some people prefer to consume fresh Klila with dates and green tea.
IMproving PULmonary hypertension Screening by Echocardiography: IMPULSE
Authors: Slegg Oliver Graham , Willis James Alexander , Wilkinson Fiona , Sparey Joseph , Wild Christopher Basil , Rossdale Jennifer , Ross Robert Mackenzie , Pauling John D. , Carson Kevin , Kandan Sri Raveen , Oxborough David , Knight Daniel , Peacock Oliver James , Suntharalingam Jay , Coghlan John Gerard , Augustine Daniel Xavier 
Published: October 2022

Abstract
Background
The world symposium on pulmonary hypertension (PH) has proposed that PH be defined as a mean pulmonary artery pressure (mPAP) > 20 mmHg as assessed by right heart catheterisation (RHC). Transthoracic echocardiography (TTE) is an established screening tool used for suspected PH. International guidelines recommend a multi-parameter assessment of the TTE PH probability although effectiveness has not been established using real world data.

Study aims
To determine accuracy of the European Society of Cardiology (ESC) and British Society of Echocardiography (BSE) TTE probability algorithm in detecting PH in patients attending a UK PH centre. To identify echocardiographic markers and revised algorithms to improve the detection of PH in those with low/intermediate BSE/ESC TTE PH probability.

Methods
TTE followed by RHC (within 4 months after) was undertaken in patients for suspected but previously unconfirmed PH. BSE/ESC PH TTE probabilities were calculated alongside additional markers of right ventricular (RV) longitudinal and radial function, and RV diastolic function. A refined IMPULSE algorithm was devised and evaluated in patients with low and/or intermediate ESC/BSE TTE PH probability.

Results
Of 310 patients assessed, 236 (76%) had RHC-confirmed PH (average mPAP 42.8 ± 11.7). Sensitivity and specificity for detecting PH using the BSE/ESC recommendations was 89% and 68%, respectively. 36% of those with low BSE/ESC TTE probability had RHC-confirmed PH and BSE/ESC PH probability parameters did not differ amongst those with and without PH in the low probability group. Conversely, RV free wall longitudinal strain (RVFWLS) was lower in patients with vs. without PH in low BSE/ESC probability group (− 20.6 ± 4.1% vs − 23.8 ± 3.9%) (P < 0.02). Incorporating RVFWLS and TTE features of RV radial and diastolic function (RVFAC and IVRT) within the IMPULSE algorithm reduced false negatives in patients with low BSE/ESC PH probability by 29%. The IMPULSE algorithm had excellent specificity and positive predictive value in those with low (93%/80%, respectively) or intermediate (82%/86%, respectively) PH probability.

Conclusion
Existing TTE PH probability guidelines lack sensitivity to detect patients with milder haemodynamic forms of PH. Combining additional TTE makers assessing RV radial, longitudinal and diastolic function enhance identification of milder forms of PH, particularly in those who have a low BSE/ESC TTE PH probability.
Extension and dynamical phases in random walkers depositing and following chemical trails
Authors: Khuntia Subhashree Subhrasmita , Chaudhuri Abhishek , Chaudhuri Debasish 
Published: October 2022

Abstract
Active walker models have proved to be extremely effective in understanding the evolution of a large class of systems in biology like ant trail formation and pedestrian trails. We propose a simple model of a random walker which modifies its local environment that in turn influences the motion of the walker at a later time. We perform direct numerical simulations of the walker in a discrete lattice with the walker actively depositing a chemical which attracts the walker trajectory and also evaporates in time. We propose a method to look at the structural transitions of the trajectory using radius of gyration for finite time walks. The extension over a definite time window shows a non-monotonic change with the deposition rate characteristic of a coil-globule transition. At certain regions of the parameter space of the chemical deposition and evaporation rates, the extensions of the walker shows a re-entrant behavior. The dynamics, characterised by the mean-squared displacement, shows deviation from diffusive scaling at intermediate time scales, returning to diffusive behavior asymptotically. A mean-field theory captures the variation of the asymptotic diffusivity.
Spleen size in homozygous sickle cell disease: trends in a birth cohort using ultrasound
Authors: Walker Thomas M , Hambleton Ian R , Mason Karlene P , Serjeant Graham 
Published: October 2022

Objectives: To provide ultrasound baselines for spleen length in homozygous sickle cell disease (HbSS) and in normal controls with a HbAA genotype. Methods: The Jamaican cohort study identified 311 babies with HbSS and 246 matched HbAA controls during the screening of 100,000 consecutive deliveries in Kingston, Jamaica from 1973 to 1981. Ultrasonography commenced in 1988 when the youngest patients were aged 6 years at which time deaths, emigrations and default had reduced the numbers to 206 HbSS and 89 controls. It continued annually until 2000. Results: The spleen was visualized in all HbAA controls but in only 1103/2138 (52%) scans in HbSS. Where available, mean splenic lengths were significantly lower in HbSS (77–103 mm in males, 70–83 mm in females) compared to normal controls (89–101 mm in males, 86–95 mm in females). Assessed by statistical modelling after adjusting for body height, the splenic ratio (splenic length/body height) declined over the age range 12–20 years in HbSS, consistent with progressive splenic fibrosis. Genetic factors known to inhibit sickling, α thalassemia and fetal hemoglobin level (HbF) significantly reduced the decline in splenic ratio. Clinical splenomegaly was an insensitive measure of splenic enlargement as only 50% of patients aged 18 years and above with spleens measuring ≥150 mm on ultrasonography had palpable spleens. Conclusions: An age-related decline in splenic length occurred in HbSS and occurred more slowly with genetic factors known to inhibit sickling. The standards provided may be of value in assessing minor degrees of subclinical acute splenic sequestration. Advances in knowledge These are the first standards available for splenic length in HbSS. They may be useful in detecting red cell sequestration, not apparent from clinical splenomegaly and also provide a model for identifying factors inhibiting vaso-occlusion.
A prototype low-cost secondary standard calorimeter for reference dosimetry with ultra-high pulse dose rates
Authors: Bass Graham A , Shipley David R , Flynn Samuel F , Thomas Russell A S 
Published: October 2022

Objectives:
Ultra-high pulse dose rate modalities present significant dosimetry challenges for ionisation chambers due to significant ion recombination. Conversely, calorimeters are ideally suited to measure high dose, short duration dose deliveries and this work describes a simple calorimeter as an alternative dosemeter for use in the clinic.

Methods:
Calorimeters were constructed featuring a disc-shaped core and single sensing thermistor encased in a 3D-printed body shaped like a Roos ionisation chamber. The thermistor forms one arm of a DC Wheatstone bridge, connected to a standard DMM. The bridge-out-of-balance voltage was calibrated in terms of temperature. A graphite-core calorimeter was calibrated in terms of absorbed dose to water (J/kg) in Co-60 and conventional 6, 10 and 15 MV X-rays. Similarly, an aluminium-core calorimeter was calibrated in a conventional 20 MeV electron beam and tested in a research high dose per pulse 6 MeV electron beam.

Results:
Calorimeters were successfully calibrated in terms of absorbed dose to water in conventional radiotherapy beams at approximately 5 Gy/min with an estimated uncertainty of ±2–2.5% (k = 2), and performed similarly in a 6 MeV electron beam delivering approximately 180 Gy/s.

Conclusions:
A simple, low-cost calorimeter traceably calibrated to existing primary standards of absorbed dose could be used as a secondary standard for dosimetry for ultra-high pulse dose rates in the clinic.

Advances in knowledge:
Secondary standard calorimeters for routine measurements are not available commercially; this work presents the basis of a simple, low-cost solution for reference dosimetry for ultra-high pulse dose rate beams.
SARS-CoV-2 and the role of airborne transmission: a systematic review
Authors: Heneghan Carl J. , Spencer Elizabeth A. , Brassey Jon , Plüddemann Annette , Onakpoya Igho J. , Oke Jason L. , Evans David H. , Conly John M. , Jefferson Tom 
Published: October 2022

Background: Airborne transmission is the spread of an infectious agent caused by the dissemination of droplet nuclei (aerosols) that remain infectious when suspended in the air. We carried out a systematic review to identify, appraise and summarise the evidence from studies of the role of airborne transmission of SARS-CoV-2. Methods: We searched LitCovid, MedRxiv, Google Scholar and the WHO Covid-19 database from 1 February 2020 to 30 May 2022 and included studies on airborne transmission. Data were dual extracted, and we assessed quality using a modified QUADAS 2 risk of bias tool. Results: We included 128 primary studies and 29 reviews on airborne SARS-CoV-2. Of the 128 primary studies, 105 (82%) reported data on RT-PCR from air samples, 28 (22%) report cycle threshold values and 36 (28%) copies per sample volume. All primary studies were observational. The research often lacked standard methods, standard sampling sizes and reporting items. We found 69 descriptions of different air samplers deployed. Of the 80 in-hospital studies that reported binary RT-PCR tests, 362/3079 air samples from 75 studies conducted in hospital ward environments were positive (median 8%, IQR=0 to 23%); 23 studies reported 74/703 RT-PCR positive air samples in the ICU setting (median 17%, IQR=0% to 38%) Thirty-eight studies reported potential air transmission in the outdoors or in the community. Twenty-six studies attempted viral culture, none of which definitively demonstrated that replication-competent SARS-CoV-2 could be recovered in the air. Conclusion: SARS-CoV-2 RNA is detectable intermittently in the air in various settings. Standardized guidelines for conducting and reporting research on airborne transmission are needed. The lack of recoverable viral culture of SARS-CoV-2 from air samples prevents firm conclusions about the definitive role of airborne transmission in SARS-CoV-2.
Hypoxic preconditioning promotes survival of human adipose derived mesenchymal stem cell via expression of prosurvival and proangiogenic biomarkers
Authors: Suryawan I Gde Rurus , Pikir Budi Susetyo , Rantam Fedik Abdul , Ratri Anudya Kartika , Nugraha Ricardo Adrian 
Published: October 2022

Background: Contributing factors for improved survival of human adipose derived mesenchymal stem cells (h-AMSCs) cultured through hypoxia preconditioning, in example apoptosis inhibition involving BCL2 and HSP27 expression, trigger signal expression (VEGF), SCF expression, OCT-4 expression, and CD44+ expression. The objective if this study was to explain the mechanism and role of hypoxic preconditioning and the optimal duration of hypoxic preconditioning exposure to improve survival of h-AMSCs. Methods: An experimental laboratory explorative study (in vitro) with hypoxic preconditioning in h-AMSCs cultures. This research was conducted through four stages. First, isolation of h-AMSCs culture from adipose tissue of patients. Second, the characterization of h-AMSCs from adipose tissue by phenotype (flowcytometry) through CD44+, CD90+ and CD45-expression before being pre-conditioned for hypoxic treatment. Third, the hypoxic preconditioning in h-AMSCs culture (in vitro) was performed with an oxygen concentration of 1% for 24, 48 and 72 hours. Fourth, observation of survival from h-AMSCs culture was tested on the role of CD44+, VEGF, SCF, OCT-4, BCL2, HSP27 with Flowcytometry and apoptotic inhibition by Tunnel Assay method. Results: The result of regression test showed that time difference had an effect on VEGF expression (p<0.001;β=-0.482) and hypoxia condition also influenced VEGF expression (p<0.001;β=0.774). The result of path analysis showed that SCF had effect on OCT-4 expression (p<0.001; β=0.985). The regression test results showed that time effects on HSP27 expression (p<0.001; β=0.398) and hypoxia precondition also affects HSP27 expression (p<0.001; β=0.847). Pathway analysis showed that BCL2 expression inhibited apoptosis (p=0.030; β=-0.442) and HSP27 expression also inhibited apoptosis (p<0,001;β=-0.487). Conclusion: Hypoxic preconditioning of h-AMSC culture has proven to increase the expression of VEGF, SCF, OCT-4, and BCL2 and HSP27. This study demonstrated and explained the existence of a new mechanism of increased h-AMSC survival in cultures with hypoxic preconditioning (O2 1%) via VEGF, SCF, OCT-4, BCL2, and HSP 27.
Does Competition Benefit Complements? Evidence from Airlines and Hotels
Authors: Forbes Silke J. , Kosová Renáta 
Published: October 2022

We analyze how changes in the market structure of one industry—airlines—affect the performance of firms in a complementary industry—hotels—using an instrumental variables strategy to account for potential correlation between unobserved shocks to both markets. We find that more intense airline competition boosts hotel performance across all standard measures: price, occupancy rate, and revenue per available room. Spillovers vary across hotel quality and passenger type: Lower-quality branded hotels serving more price-sensitive travelers, most likely brought into the market because of more intense airline competition, benefit the most. However, performance spillovers do not translate into higher hotel entry. This paper was accepted by Joshua Gans, business strategy. Supplemental Material: The data files are available at https://doi.org/10.1287/mnsc.2022.4568 .
A Macrofinance Model for Option Prices: A Story of Rare Economic Events
Authors: Hasler Michael , Jeanneret Alexandre 
Published: October 2022

We propose a macrofinance model that rationalizes robust features in equity index option markets. When rare disasters are followed by economic recoveries, the slope of the implied volatility term structure is positive in good times but turns negative in bad times. Additionally, implied volatility decreases with moneyness in bad times (volatility skew), whereas the shape becomes a smile in good times in the presence of rare economic booms. Our theory contributes to understanding the dynamics of the implied volatility surface yet keeping standard asset-pricing moments realistic. This paper was accepted by Gustavo Manso, finance. Funding: The authors are grateful to HEC Montreal, the University of Texas at Dallas, and particularly to the Canadian Derivatives Institute for generous financial support. Supplemental Material: The online appendix and data are available at https://doi.org/10.1287/mnsc.2022.4587 .