bluuebunny/crossref_metadata_2025_split huggingface dataset:
Youth Research in Community Settings: Inspiring Social Engagement Through Critical Pedagogy, Collaboration, and Arts-Based Research
Authors: Kreikemeier Alyssa 
Published: May 2022

Background/Context: This article shares qualitative findings from one arts-based youth research project. It offers insights for educators and researchers working in youth-driven contexts with an interest in critical pedagogy, engaged research methods, and youth media. Purpose and Research Questions: Youth artist-researchers produced their own media projects that explored themes of importance to them as part of a critical media literacy project. The class was designed around the research question: What role do narratives, representation, and media-making play in the identity construction of youth in an urban setting? Research Design: The arts-based research project, Through the Lens of Resistance, involved six youth artist-researchers who produced media artworks during the summer class and presented their work in community spaces. I led the class and collected data, serving as both teaching artist and lead researcher. The installations explored racialized violence, representation, and the political work of media. Youth researchers learned critical media theory and media-based qualitative methods from a Global Action Project curriculum over 4 weeks. In the final 2 weeks, youths worked individually or in groups to design an arts-based installation that involved media research. After the conclusion of the class, two artists continued to show one of the final projects in various community settings. Qualitative data shared here come from interviews, participant observation, and one focus group. Findings/Results: This article identifies the potential of qualitative methods that foreground critical pedagogy and art-making to generate youth community engagement and critical consciousness. It shares insights for educators and researchers working in youth-driven contexts. This article demonstrates that arts-based research grounded in critical pedagogy and community spaces offers a powerful vehicle for young people to develop critical consciousness as they grapple with social issues.
From dementia mindsets to emotions and behaviors: Predicting person-centered care in care professionals
Authors: Kunz Lena K , Scheibe Susanne , Wisse Barbara , Boerner Kathrin , Zemlin Claudia 
Published: May 2022

Background and Objective High-quality care standards for dementia care are increasingly based on person-centered care principles. To better understand facilitating factors of person-centered care this research focuses on individual characteristics of care professionals. Applying mindset theory to dementia care, we examined dementia mindsets (viewing dementia symptoms as either malleable or fixed) in care professionals. We tested whether there is a positive relationship between a malleable dementia mindset and person-centered care as well as a negative relationship between a fixed dementia mindset and person-centered care. Moreover, we examined whether care professionals’ emotional responses in care situations help explain associations between dementia mindsets and person-centered care. Research Design and Method In two cross-sectional studies, care professionals of long-term care facilities (total N = 370) completed a measure of dementia mindsets and reported their emotional and behavioral responses to five care scenarios. Regression and mediation analyses were performed. Findings The tested hypotheses were partially supported. A fixed dementia mindset predicted reported person-centered care negatively, while a malleable dementia mindset did not. Mediation analyses suggest that reduced negative emotions may underlie the association between a malleable mindset and reported person-centered care, while reduced positive emotions in care situations may underlie the association of a fixed mindset and reported person-centered care. Study 2 partially replicated these findings. A fixed mindset and positive emotional responses were the most robust predictors of reported person-centered care. Discussion and Implications This study extends knowledge on facilitators (positive emotional responses to care situations) and barriers (fixed dementia mindset) to person-centered care in care professionals working with persons with dementia. We discuss how dementia mindsets and emotional responses to care situations may be a fruitful target for trainings for care professionals.
Identification of condition-specific regulatory mechanisms in normal and cancerous human lung tissue
Authors: Hang Yuqing , Burns Josh , Shealy Benjamin T. , Pauly Rini , Ficklin Stephen P. , Feltus Frank A. 
Published: May 2022

Abstract
Background
Lung cancer is the leading cause of cancer death in both men and women. The most common lung cancer subtype is non-small cell lung carcinoma (NSCLC) comprising about 85% of all cases. NSCLC can be further divided into three subtypes: adenocarcinoma (LUAD), squamous cell carcinoma (LUSC), and large cell lung carcinoma. Specific genetic mutations and epigenetic aberrations play an important role in the developmental transition to a specific tumor subtype. The elucidation of normal lung versus lung tumor gene expression patterns and regulatory targets yields biomarker systems that discriminate lung phenotypes (i.e., biomarkers) and provide a foundation for the discovery of normal and aberrant gene regulatory mechanisms.

Results
We built condition-specific gene co-expression networks (csGCNs) for normal lung, LUAD, and LUSC conditions. Then, we integrated normal lung tissue-specific gene regulatory networks (tsGRNs) to elucidate control-target biomarker systems for normal and cancerous lung tissue. We characterized co-expressed gene edges, possibly under common regulatory control, for relevance in lung cancer.

Conclusions
Our approach demonstrates the ability to elucidate csGCN:tsGRN merged biomarker systems based on gene expression correlation and regulation. The biomarker systems we describe can be used to classify and further describe lung specimens. Our approach is generalizable and can be used to discover and interpret complex gene expression patterns for any condition or species.
A novel and sensitive DNA methylation marker for the urine-based liquid biopsies to detect bladder cancer
Authors: Deng Leihong , Chao Haichao , Deng Huanhuan , Yu Zhaojun , Zhao Rongsong , Huang Longwu , Gong Yun , Zhu Yueting , Wang Qingping , Li Feng , Liu Lirong , He Lei , Tang Zhimin , Liao Caizhi , Qi Yan , Wang Xianshu , Zeng Tao , Zou Hongzhi 
Published: May 2022

Abstract
Background
Better prognostic outcome is closely correlated with early detection of bladder cancer. Current non-invasive urianalysis relies on simultaneously testing multiple methylation markers to achieve relatively high accuracy. Therefore, we have developed an easy-to-use, convenient, and accurate single-target urine-based DNA methylation test for the malignancy.

Methods
By analyzing TCGA data, 344 candidate markers with 424 primer pairs and probe sets synthesized were systematically screened in cancer cell lines, paired tissue specimens, and urine sediments from bladder cancer patients and normal controls. The identified marker was further validated in large case-control cohorts. Wilcoxon rank sum tests and c2 tests were performed to compare methylation levels between case-control groups and correlate methylation levels with demographic and clinical characteristics. In addition, MSP, qMSP, RT-PCR, western blot analysis, and immunohistochemistry were performed to measure levels of DNA methylation, mRNA transcription, and protein expression in cancer cell lines and tissues.

Results
A top-performing DMRTA2 marker identified was tested in both discovery and validation sets, showing similar sensitivity and specificity for bladder cancer detection. Overall sensitivity in the aggregate set was 82.9%(179/216). The specificity, from a control group consisting of patients with lithangiuria, prostatoplasia, and prostatitis, is 92.5%(468/506). Notably, the methylation assay had the highest sensitivities for tumors at stages of T1(90.4%) and T2(95.0%) compared with Ta (63.0%), T3(81.8%), and T4(81.8%). Furthermore, the test showed admirable detection rate of 80.0%(24/30) for recurring cancers. While methylation was observed in 39/54(72.2%) urine samples from patients with carcinomas of renal pelvis and ureter, it was detected at extremely low rate of 6.0%(8/133) in kidney and prostate cancers. Compared with SV-HUC-1, the normal bladder epithelial cell line, DMRTA2 was hypermethylated in 8/9 bladder cancer cell lines, consistent with the results of MSP and qMSP, but not correlated with mRNA and protein expression levels in these cell lines. Similarly, DMRTA2 immunostaining was moderate in some tissues but weak in others. Further studies are needed to address functional implications of DMRTA2 hypermethylation.

Conclusions
Our data demonstrated that a single-target DNA methylation signature, mDMRTA2, could be highly effective to detect both primary and recurring bladder cancer via urine samples.
Trends in educational disparities in preventive behaviours, risk perception, perceived effectiveness and trust in the first year of the COVID-19 pandemic in Germany
Authors: Pförtner Timo-Kolja , Dohle Simone , Hower Kira Isabel 
Published: May 2022

Abstract
Background
Educational disparities in health and health behaviours have always been relevant in public health research and are particularly challenging in the context of the COVID-19 pandemic. First studies suggest that factors important for the containment of the COVID-19 pandemic, such as prevention behaviour, risk perception, perceived effectiveness of containment measures, and trust in authorities handling the pandemic, vary by educational status. This study builds on recent debate by examining trends in absolute and relative educational disparities in these factors in the first year of the COVID-19 pandemic in Germany.

Methods
Data stem from four waves of the GESIS Panel surveyed between March and October 2020 in Germany (15,902 observations from 4,690 individuals). Trends in absolute and relative disparities were examined for preventive behaviour, risk perception, perceived effectiveness of COVID-19 containment measures, and trust in individuals and institutions handling the COVID-19 pandemic by educational status using sex, age, residence, nationality, children under 16 living in household, family status, household size, the Big Five Inventory, and income class as control factors. Descriptive statistics as well as unadjusted and adjusted linear regression models and random effects models were performed.

Results
We observed an initially rising and then falling trend in preventive behaviour with consistent and significant absolute and relative disparities with a lower preventive behaviour among low educated individuals. Indication of a U-shaped trend with consistent significantly lower values among lower educated individuals was found for risk perception, whereas perceived effectiveness and trust decreased significantly over time but did not significantly vary by educational status.

Conclusions
Results indicate persistent educational disparities in preventive behaviour and risk perception and a general decline in perceived effectiveness and trust in the first year of the COVID-19 pandemic in Germany. To address this overall downward trend and existing disparities, comprehensive and strategic management is needed to communicate the risks of the pandemic and the benefits of COVID-19 containment measures. Both must be adapted to the different needs of educational groups in particular in order to overcome gaps in preventive behaviour and risk perception by educational status.
Successful management of a patient with ovarian ectopic pregnancy by the end of the first trimester: a case report
Authors: Kasraei Sara , Seifollahi Akram , Aghajani Faezeh , Nakhostin-Ansari Amin , Zarei Neda , Tehranian Afsaneh 
Published: May 2022

Abstract
Background
Among all ectopic pregnancies, between 0.5% and 3.5% are ovarian ectopic pregnancies, a potentially life-threatening condition when ruptured due to its serious potential for hemorrhaging. A majority of ovarian ectopic pregnancies are diagnosed by the 7th week of pregnancy when the patient becomes symptomatic, and ultrasound can be used to diagnose this condition.

Case presentation
We present the case of a 39-year-old Persian woman in the 12th week of gestation who presented with vaginal bleeding and abdominal pain and was diagnosed with ovarian ectopic pregnancy. Her notable laboratory finding was β-human chorionic gonadotropin > 15,000, which indicates definite pregnancy. Transvaginal ultrasound (TVS) revealed no evidence of intrauterine pregnancy, but a well-circumscribed gestational sac in the left ovary. The patient was successfully treated with resection of the gestational sac and partial left salpingo-oophorectomy. Histopathological studies confirmed the diagnosis of ovarian ectopic pregnancy.

Conclusion
The case emphasizes the ability of ovarian ectopic pregnancy to develop asymptomatically through the course of pregnancy and points to the necessity for high-quality prenatal care and the importance of determining the fetal site during pregnancy.
Favorable effect of enhanced recovery programs on post-discharge mortality: a French nationwide study
Authors: Slim Karem , Boudemaghe Thierry , Delaunay Laurent , Léger Lucas , Bizard Frédéric 
Published: May 2022

Abstract
Background
Enhanced recovery programs (ERPs) imply early discharge but few papers have assessed the effect of ERPs on post-discharge mortality (PDM).

Methods
A multicenter nationwide case control study based on administrative data was carried out between March and December 2019. Coding for every episode of care whether in the setting of ERP or not is mandatory for hospital funding (public or private). Twelve surgical specialties or procedures were included. The episodes of care coded with ERP were matched with those without ERP code for several factors such as the type of hospital (public or private), age, gender, month of discharge, and updated Charlson score. Ninety-day PDM was the main outcome.

Results
Of 420,031 patients in the database, 78,119 had an ERP code. Finally, 132,600 patients with 66,300 matched pairs were considered for the study. Overall, PDM was significantly reduced after ERPs: 0.075% vs 0.138% (p = 0.00042). Significant one-half and two-thirds reduction in PDM was observed respectively after hip arthroplasty (odds ratio 0.48 [95% CI 0.21–0.99]) and colectomy (odds ratio 0.36 [95% CI 0.16–0.74]).

Conclusion
The findings, based on a large database and a rigorous matching, strongly suggest that ERPs reduce PDM particularly after colectomy and hip arthroplasty. This is likely due to better post-operative care in ERPs.
Stepping up to meet the challenge of freezing of gait in Parkinson’s disease
Authors: Lewis Simon , Factor Stewart , Giladi Nir , Nieuwboer Alice , Nutt John , Hallett Mark 
Published: May 2022

AbstractThere has been a growing appreciation for freezing of gait as a disabling symptom that causes a significant burden in Parkinson’s disease. Previous research has highlighted some of the key components that underlie the phenomenon, but these reductionist approaches have yet to lead to a paradigm shift resulting in the development of novel treatment strategies. Addressing this issue will require greater integration of multi-modal data with complex computational modeling, but there are a number of critical aspects that need to be considered before embarking on such an approach. This paper highlights where the field needs to address current gaps and shortcomings including the standardization of definitions and measurement, phenomenology and pathophysiology, as well as considering what available data exist and how future studies should be constructed to achieve the greatest potential to better understand and treat this devastating symptom.
Examining classroom writing assessment literacy: a focus on in-service EFL teachers in Iran
Authors: Tayyebi Masoumeh , Abbasabady Mahmoud Moradi , Abbassian Gholam-Reza 
Published: May 2022

AbstractWriting assessment literacy (WAL) has received research attention over the past few years. This study aimed at investigating writing assessment knowledge of Iranian English language teachers along with their conceptions and practices of writing assessment based on Crusan et al.’s (Assessing Writing 28:43–56, 2016) study in order to have a better understanding of their current situation and to predict and accommodate for future writing assessment requirements. The study further aimed at examining how teachers’ knowledge, conception, and practice of writing assessment are influenced by contextual and experiential factors. To accomplish this goal, a test of writing assessment knowledge together with an adapted version of a questionnaire developed by Crusan et al. (Assessing Writing 28:43–56, 2016) for writing assessment conceptions and practice was distributed among 120 Iranian in-service teachers selected based on convenience sampling. The results of the study have shown inadequate levels of writing assessment knowledge for participating teachers. With regard to the conceptions of writing assessment, majority of the participants valued innovative assessment methods like portfolio and self/peer assessment methods although in practice, they rarely used these methods. The study revealed no impact of teaching experience and context on teachers’ writing assessment knowledge and practice. The findings of this study contribute to our current understanding of WAL development and provide a more accurate picture of writing assessment training needs of Iranian teachers and the development of more efficient teacher education courses.
Impact of retraction force magnitudes on mobility of maxillary canines: a split-mouth design
Authors: Albelasy Nehal F. , Abdelnaby Yasser L. 
Published: May 2022

Abstract
Objective
Prospective evaluation of the maxillary canine mobility during retraction using two different force levels over 5 months of retraction.

Materials and methods
Thirty patients indicated for maximum retraction of maxillary canines with age range of 14.7–18.9 years were included in the study. After complete leveling and alignment and immediately before canine retraction, the mobility of the maxillary canines was measured using the Periotest device and repeated monthly. A split-mouth design was applied where on the one side, the retraction force was 100 g, while on the other side 200 g of force. Four subgroups were investigated: A1 (R3 100 g), A2 (L3 200 g), B1 (R3 200 g) and B2 (L3 100 g). The total amount of canine retraction was measured for each side using the pre- and post-retraction dental casts.

Results
The collected data were normally distributed. ANOVA test showed insignificant statistical difference in Periotest values (PTVs) among the four subgroups pre-retraction and monthly p > 0.05. However, each group showed a statistically significant difference in PTVs over the 5 months. The independent sample t test showed a statistical insignificant difference in PTVs between the 100 g and 200 g retraction force. Pearson correlation of the PTVs to the period of retraction was statistically significant p < 0.05 while being in significant to the retraction force p > 0.05.

Conclusion
Increasing the retraction force of maxillary canines up to 200 g of force does not significantly increase the teeth mobility during orthodontic treatment. There is a positive correlation between the PTVs and the duration of tooth movement regardless the magnitude of force.
Comprehensive analysis of DNA methylation for periodontitis
Authors: Zhao Zengbo , Wang Huimin , Li Xiaona , Hou Jingya , Yang Yuntian , Li Hexiang 
Published: May 2022

AbstractBackgroundPeriodontitis is an infectious disease, and a risk factor for peri-implantitis that could result in the implant loss. DNA methylation has an essential role in the etiology and pathogenesis of inflammatory disease. However, there is lack of study on methylation status of genes in periodontitis. This study sought to explore the gene methylation profiling microarray in periodontitis.MethodsThrough searching in the Gene Expression Omnibus database, a gene methylation profiling data set GSE173081 was identified, which included 12 periodontitis samples and 12 normal samples, respectively. Thereafter, the data of GSE173081 was downloaded and analyzed to determined differentially methylated genes (DMGs), which then were used to perform Gene Ontology analysis and pathway enrichment analyses through online database. In addition, the DMGs were applied to construct the protein–protein interaction (PPI) network information, predict the hub genes in pathology of periodontitis.ResultsIn total 668 DMGs were sorted and identified from the data set, which included 621 hypo-methylated genes and 47 hyper-methylated genes. Through the function and ontology analysis, these 668 genes are mainly classified into intracellular signaling pathway, cell components, cell–cell interaction, and cellular behaviors. The pathway analysis showed that the hypo-methylated genes were mostly enriched in the pathway of cGMP–PKG signaling pathway; RAF/MAP kinase; PI3K–Akt signaling pathway, while hyper-methylated genes were mostly enriched in the pathway of bacterial invasion of epithelial cells; sphingolipid signaling pathway and DCC mediated attractive signaling. The PPI network contained 630 nodes and 1790 interactions. Moreover, further analysis identified top 10 hub genes (APP; PAX6; LPAR1; WNT3A; BMP2; PI3KR2; GATA4; PLCB1; GATA6; CXCL12) as central nodes that are involved in the immune system and the inflammatory response.ConclusionsThis study provides comprehensive information of methylation status of genes to the revelation of periodontitis pathogenesis that may contribute to future research on periodontitis.
Online self-evaluation: the EFL writing skills in focus
Authors: Al-Mwzaiji Khaled Nasser Ali , Alzubi Ali Abbas Falah 
Published: May 2022

AbstractStudents need more independent roles to evaluate their learning performance, especially in the absence of teachers’ feedback due to some constraints such as technology and poor experience. This study investigates the potential of online self-evaluation on students’ writing skills in English as a foreign language (EFL). It examines the preparatory year (PY) level one students’ most improved EFL writing areas and perceptions when they use online self-evaluation in writing. In this quasi-experiment, two groups of PY EFL writing program students (N = 60) participated in the study for the first semester 2020–2021. A mixed-method design was applied: Self-monitoring checklists, self-evaluation questionnaires, achievement tests, and students’ portfolios. Findings revealed that most of the learners’ mistakes were in punctuation marks, capitalization, informal language, and subject-verb agreement. It was also perceived that learners were doing fine about where and how they should be. The study proved that the students’ self-evaluation and performance in writing correlated significantly; however, the effect size was low. Based on the research findings, it is recommended that training programs on strategy are to be boosted, especially in institutions where learners receive less feedback.