bluuebunny/crossref_metadata_2025_split huggingface dataset:
The complete chloroplast genome sequences of Delavaya toxocarpa and Harpullia cupanioides (Dodonaeoideae, Sapindaceae)
Authors: Cao Limin , Wang Zhixin , Jiang Weimin , Cao Ming 
Published: April 2022

Abstract
The complete chloroplast (cp) genomes of Delavaya toxocarpa and Harpullia cupanioides were reported here. In D. toxocarpa, the total genome size was 161,325 bp in length, consisting of two IRs (26, 698 bp) separated by the LSC (88, 698 bp) and SSC (19, 231 bp) regions. The genome contained 131 genes, including 85 protein-coding genes, 37 tRNA genes and 8 rRNA genes respectively. The GC-content of the complete chloroplast genome was 37.5%. As to H. cupanioides, the complete chloroplast genome was 159, 776 bp in length, consisting of two IRs (26, 938 bp) separated by the LSC (87, 804 bp) and SSC (18, 096 bp) regions. The genome contained 131 genes, including 85 protein-coding genes, 37 tRNA genes and 8 rRNA genes respectively. The GC-content of the complete chloroplast genome was 37.8%. A phylogenetic analysis indicated that D. toxocarpa was closely related to Koelreuteria, and H. cupanioides was close with Dodonaea viscosa. This study will be useful for the phylogenetic position determination as well as future phylogenetic study of Delavaya toxocarpa and H. cupanioides.
Clinical application of next-generation sequencing-based monitoring of minimal residual disease in childhood acute lymphoblastic leukemia
Authors: Mai Huirong , Li Qin , Wang Guobing , Wang Ying , Liu Shilin , Tang Xue , Chen Fen , Zhou Guichi , Liu Yi , Li Tonghui , Wang Lulu , Wang Chunyan , Wen Feiqiu , Liu Sixi 
Published: April 2022

Abstract
Background: Next-generation sequencing (NGS) is an emerging technology that can comprehensively assess the diversity of the immune system. We explored the feasibility of NGS in detecting minimal residual disease (MRD) in childhood acute lymphoblastic leukemia (ALL) based on immunoglobulin and T cell receptor.Methods: Bone marrow samples were collected pre- and post-treatment with pediatric ALL admitted to Shenzhen Children's Hospital from February 1st, 2020 to January 31st, 2021. We analyzed the MRD detected by NGS, multiparametric flow cytometry (MFC) and real-time quantitative PCR (RQ-PCR), and analyzed risk factors of positive NGS-MRD at the end of B-ALL induction chemotherapy.Results: A total of paired 236 bone marrow samples were collected from 64 children with ALL (58 B-ALL and 6 T-ALL). The decrease in the clonal rearrangement frequency of IGH, IGK, and IGL was generally consistent after treatment. Positive MRD was detected in 57.5% (77/134) of B-ALL and 80% (12/15) of T-ALL by NGS after chemotherapy, which was higher than those detected by MFC and RQ-PCR. In B-ALL patients, MRD results detected by NGS were consistent with MFC（r = 0.708, p < 0.001）and RQ-PCR（r = 0.618, p < 0.001）. At the end of induction, NGS-MRD of 40.4% B-ALL was >0.01% and multivariate analysis indicated that ≧2 clonal rearrangement sequences before treatment were an independent factor of negative NGS-MRD. Conclusions: NGS is more sensitive than MFC and RQ-PCR for MRD measurement. B-ALL children with ≧2 clonal rearrangements detected by NGS before treatment are difficult to switch to negative MRD after chemotherapy.
Association between Genetic Risk Score and Tri-ponderal Mass Index Growth Trajectories among Different Dietary Consumption Adolescents in a Prospective Taiwanese Cohort
Authors: Wu Yi-Fan , Chien Kuo-Liong , Chen Yang-Ching 
Published: April 2022

Abstract
Background: Single-nucleotide polymorphisms (SNPs) in various genetic loci are associated with childhood obesity; however, their influence on adolescent growth patterns has rarely been explored. This study investigated whether genetic variants could predict tri-ponderal mass index (TMI)-derived growth trajectories and the interaction between genetic and dietary factors.Methods: We conducted Taiwan Puberty Longitudinal Study, a prospective cohort that recruited 1,135 children since 2018. Anthropometric measurements were recorded every three months, while dietary nutrition assessment and biological sampling for genotyping were collected during the first visit. TMI growth trajectory groups were identified using growth mixture modeling. A multinomial logistic regression model for different growth trajectories was used to examine the effect of candidate SNPs, and the most related SNPs were used to establish the genetic risk score. We then explored the effect of the genetic risk score in subgroup analysis according to dietary calories and different dietary consumption patterns.Results: Three TMI-based growth trajectory groups were identified among adolescents. The “increased weight” trajectory group accounted for approximately 9.7% of the participants. FTO/rs7206790 was associated with the increased weight growth trajectory after adjusting for the baseline TMI and other correlated covariates (odds ratio: 2.13, 95% confidence interval: 1.08–4.21). We generated the genetic risk score by using 4 SNPs (FTO/rs7206790, ADCY9/rs2531995, TFAP2B/rs4715210, and TMEM18/rs6548238) and selected the threshold of 10 points to define risk categories. There were 11.66% and 3.24% of participants belonged to the increased weight trajectory in high- and low-risk groups, respectively; and the predictive ability of the genetic risk score was notable among low calories intake participants (odds ratio: 1.90, 95% confidence interval: 1.18-3.05 vs. odds ratio: 1.17, 95% confidence interval: 0.78-1.75 in high calories intake group).Conclusion: Our results offer a new perspective on the genetic and dietary basis of changes in adolescent obesity status. Individualized interventions for obesity prevention may be considered among high-risk children.
Assessment of learning a new skill using non-linear and spectral features of EEG
Authors: Goshvarpour Ateke , Jalaly Fateme 
Published: April 2022

Abstract
The goal of this paper was to assess the impact of learning a new skill on several electroencephalographic (EEG) measurements. In addition, it intended to examine the active brain regions associated with learning. To this effect, changes in brain activity pre- and post-learning task was investigated using EEG power spectral density (PSD) and nonlinear features. The evaluated task was learning to type using the Colemak keyboard layout in a twelve-lesson training. 10 participants with a mean age of 29.3 ± 5.7 were included in the experiment. For the first time, Fractal dimension, approximate entropy, and Lyapunov exponents were extracted from a 9-channel EEG signal to characterize brain dynamics. In addition, the PSD of various EEG rhythms was estimated. To identify statistically significant changes in the EEG characteristics, as well as to determine the prominent channels, the t-test was performed. Our results showed maximum EEG power in Beta and Gamma waves; however, the most differences in the power distribution belonged to the Beta band. Among non-linear features, entropy showed a significant difference in 70% of the EEG channels. This has mainly occurred in the F3, Fz, C3, Cz, POz, and P4 electrodes. Taken together, the results of this study pave the way to evaluate brain dynamics and actively involved areas pre- and post-learning tasks.
Effects of an enriched environment on the intestinal mucosal barrier and brain gut axis in colorectal cancer model rats
Authors: Liu Dun , Wu Xian-yi , Huang Si-ting , Yu Xin-yuan , Wu Yu-xuan 
Published: April 2022

Abstract
Objectives: Environmental enrichment (EE) involves introducing animals to novel, complex, and stimulating surroundings, which can protect the intestinal mucosal barrier and regulate brain–gut peptide expression. However, the effects of EE and a normal environment have not been compared in rats, and to what extent EE can protect the injured intestinal mucosal barrier is unknown. Herein, the effects of probiotics, EE, and joint intervention targeting the intestinal mucosal barrier and brain–gut axis were assessed in rats with colorectal cancer.Methods: Each rat was weighed, and rats were housed under four different conditions for 4 weeks: an enriched environment, colorectal cancer modeling, joint conditions and normal conditions. We used a rat model of 1,2-dimethylhydrazine (DMH)-induced colorectal cancer. The intestinal mucosal and serum levels of TNF-α, IL-6, IL-10, DAO, ATP, CRF, and occludin and bacterial translocation (BT) were measured, and the intestinal mucosal morphology was evaluated.Results: EE was beneficial for serum and intestinal mucosal cytokine, hypothalamic CRF, substance P, and occludin levels; villus length; and muscle thickness (P<0.05). There were no significant differences in villus width or BT between the enriched environment group and other groups (P>0.05).Conclusions: Colorectal cancer can damage the intestinal mucosa, but EE is conducive to protecting the intestinal mucosa in colorectal cancer and healthy conditions. EE is helpful in regulating brain–gut peptides. In future research, the effect of EE on the physical and biological barriers established by the intestinal mucosa and the mechanism of EE related to the intestinal mucosa should be explored.
HMSCs exosome-derived miR-199a-5p attenuates sulfur mustard-associated oxidative stress via the CAV1/NRF2 signaling pathway
Authors: Gong Chuchu , Xu Qingqiang , Zhang Xinkang , Mao Guanchao , Pei Zhipeng , Meng Wenqi , Cen Jinfeng , Gu Zhengyan , Liu Jihao , He Xiaowen , Sun Mingxue , Xiao Kai 
Published: April 2022

Abstract
Background: Sulfur mustard (SM) is a blister-producing chemical warfare agent which could lead to a cascade of systemic damage, especially severe acute lung injury. Oxidative stress is considered to be vital processes for the SM toxicity mechanism. We previously proved the therapeutic effect of exosomes derived from bone marrow mesenchymal stromal cells in promoting the repair of alveolar epithelial barrier and inhibiting apoptosis. However, the key functional components in exosomes and the underlying mechanisms have not been fully elaborated. This research shed light on the function of the key components of human umbilical cord mesenchymal stem cell-derived exosomes. Results: We noted that HMSCs-Ex showed better antioxidant abilities to attenuate SM-induced apoptosis. Furthermore, it was identified that HMSCs-Ex-derived miR-199a-5p played the vital role in reducing pneumonocyte oxidative stress. We observed that the overexpression of miR-199a-5p in HMSCs-Ex exerted protective effects against SM-induced oxidative injury by activating the NRF2 signaling pathway. Moreover, it was confirmed that Caveolin1 (CAV1) was the target gene regulated by miR-199a-5p. MiR-199a-HMSCs-Ex reduced CAV1 expression, leading to the upregulation of NRF2 and the activation of the NRF2 antioxidant signal pathway.Conclusions: MiR-199a-5p was one of the key molecules in HMSCs-Ex that attenuated SM-associated oxidative stress via regulating CAV1/NRF2 signaling pathway.
Numerical modelling, parametric analysis and design of UHPFRC beams exposed to fire
Authors: Simwanda Lenganji , Kahanji Charles , Ali Faris 
Published: April 2022

Abstract
A non-linear sequentially-coupled thermal-mechanical finite element (FE) paradigm that reasonably tracks the coupled fire-structure response of ultra-high performance fibre reinforced concrete (UHPFRC) beams subjected to the ISO 834 fire is established and presented. A fire resistance parameter study is conducted on UHPFRC beams via the developed FE paradigm, and then flexural based fire-structural assessment is demonstrated, as an avenue to substantiate the viability of numerical performance-based structural fire design The parameter study considered two variables, notably heating mechanism and load levels. Comparison of results revealed that the fire-structure response was more severe under the hydrocarbon than the ISO834 heating mechanism and hydrocarbon fire resistance ratings estimation were found to be 30 minutes lower than those determined using the ISO 834 fire. Predicted failure of the beams under the ISO 834 and hydrocarbon fires was observed at load ratios exceeding 0.6 and 0.4, respectively. By reposing on ACI544’s analytical model for UHPFRC beams in flexure, and numerically acquired heat-transfer response, the time-variant reduced nominal moment capacities of UHPFRC beams were computed. Failure predicted via computed residual moment capacities was somewhat consistent with that reckoned by load ratio parameter study.
The emerging role of anti-PD-1 antibody-based regimen in the treatment of extranodal NK/T-cell lymphoma-associated hemophagocytic lymphohistiocytosis
Authors: Yanxia He , Gao Yan , ping Liqin , He Haixia , Huang Cheng , Bai Bing , Wang Xiaoxiao , Li Zhi-Ming , Cai Qingqing , Huang Yuhua , Pan Xueyi , Zeng Wenbin , liu Yanan , Huang Huiqiang 
Published: April 2022

Abstract
Purpose
Anti-PD-1 antibody (anti-PD-1 mAb) showed favourable outcomes in some patients with relapsed/refractory (r/r) extranodal NK/T-cell lymphoma (ENKTL). However, the role of anti-PD-1 antibody in NK/T-cell lymphoma-associated hemophagocytic lymphohistiocytosis (NK/T-LAHS) remains unclear. Here, we evaluated the efficacy and toxicity of anti-PD-1 antibody-based treatment in patients with NK/T-LAHS.
Methods
The clinical data of 98 NK/T-LAHS patients diagnosed with NK/T-LAHS at Sun Yat-sen University Cancer Center and the First Affiliated Hospital of Guangdong Pharmaceutical University from May 2014 to November 2021were retrospectively analysed. All patients received anti-HLH (HLH-2004 [etoposide, dexamethasone, cyclosporine A] or DEP-based [liposomal doxorubicin, etoposide, methylprednisolone]) regimen and sequential anti-ENKTL chemotherapy (ChT) plus anti-PD-1 antibody or not.
Results
The overall response rate (ORR) of the anti-PD-1 mAb plus ChT regimens was higher than that of the ChT regimens (73.3% vs. 45.5%, P = 0.041). The toxicity of the anti-PD-1 mAb plus ChT regimens was tolerable. Except for higher rates of neutropenia, no significant difference in adverse events (AEs) was observed between the two groups. When the best ORR of anti-ENKTL was achieved, the median EBV DNA levels in patients who received anti-PD-1 mAb plus ChT were significantly lower than patients who received ChT only (878 copies/ml vs. 18600 copies/ml, P = 0.001). With a median follow-up of 26.6 months (range, 0-65.9 months), the median overall survival (mOS) was 3.5 months (95% CI: 2.3–4.7 months). Patients treated with anti-PD-1 mAb plus ChT experienced a longer mOS than those who received ChT only (5.2 months [95% CI: 2.5–7.8 months] vs. 1.5 months [95% CI: 0.5–2.6 months], P = 0.002). Cox multivariate analysis found that anti-PD-1 mAb was an independent prognostic factor for all NK/T-LAHS patients.
Conclusion
In conclusion, anti-PD-1 mAb combined with ChT regimens seemed to be associated with prolonged survival in NK/T-LAHS patients and may represent a potentially promising treatment strategy for this population.
Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. Professor Blaauw and genetic fidelity studies using SCOT markers
Authors: Verma Vipasha , Kumar Akhil , Chaudhary Priti , Chauhan Seema , Thakur Meenakshi , Bhargava Bhavya 
Published: April 2022

Abstract
Dutch Iris is a commercially important bulbous ornamental crop. Its high demand in global floriculture market necessitates the production of high-quality planting material. In the present investigation, the significance of meta-topolin (mT), a novel aromatic cytokinin, was studied on in vitro morphogenesis of Iris × hollandica Tub. cv. Professor Blaauw (Dutch Iris). Cytokinin mT (1.0 mg L− 1) as a better substitute to BAP (6-Benzylaminopurine) and Kn (Kinetin), has resulted in maximum shoot induction response (86.11 per cent) and increased emergence of micro shoots (4.95 shoots/explant with average shoot length of 5.65 cm) from twin scale explants. Photoperiod also affected the shoot induction, wherein initial 1-week (w) dark incubation significantly increased shoot induction response (94.44 per cent). Both meta-topolin and initial 1w dark incubation resulted in improved quality of regenerated shoots by reducing the incidence of necrosis and preventing vitrification. The collective consequence of cytokinin-auxin (1.0 mg L− 1 mT + 0.25 mg L− 1 NAA) showed considerably higher number of shoots (17.53) with mean shoot length (6.90 cm) and maximum number of bulbets (2.74). Positive effect of increased sucrose concentration (90 g L1) and paclobutrazol (5 mg L1) on in vitro bulblet formation and bulblet size was also observed. The superiority of mT over BAP was also found during in vitro rhizogenesis. Shoots derived from mT medium were healthy and long enough, thus showed better rooting response (63.3 %) on ½ MS medium + 0.5 mg L− 1 IAA after 4 weeks. The mT raised plantlets were acclimatized in a greenhouse and showed 89.6 % survival under ex vitro conditions The clonal fidelity of the in vitro regenerated plants was evaluated using Start Codon Targeted (SCoT) markers. Out of 36 primers, 13 primers showed clearly scorable monomorphic bands, thus displaying genetic uniformity among in vitro regenerated plantlets. This meta-topolin mediated protocol can be routinely used for the rapid large scale production of this valuable floriculture crop.
On sustainable self-curing self-compacting concrete using polyethylene glycol and manufactured sand
Authors: Bhojaraju Chandrasekhar , Balasubramanya Manjunath , Mousavi Seyed Sina , Bhagithimar Yajnheswaran 
Published: April 2022

Abstract
Self-compacting concrete (SCC) is commonly used when compaction is challenging, requiring special attention towards curing. Conventional curing techniques often fail in practice. Water evaporation is often reduced even when precise control measures are taken, but surface water on vertical structure elements remains a problem. To address these challenges, this study intends to determine the possibility of achieving self-curing self-compacting concrete (SCSCC). As curing agent plays a significant role in producing SCSCC, this study concentrates on assessing the impact of polyethylene glycol (PEG) addition at different rates of 0.5%, 1%, 1.5%, and 2% on the fresh, hardened, durability characteristics. Moreover, to improve the sustainability properties of SCSCC, manufactured sand (M-sand) obtained from rock crushing operations is used as a replacement for river sand. Generally, results indicate that using superplasticizer and M-sand is sufficient for achieving the required flowability for SCC mixtures without using specific fillers, promptly controlling bleeding and segregation, and maintaining the necessary compressive strength at all ages. The hardened properties of SCSCC were improved by increasing PEG content up to 1.5% with an optimal range of 0.5% superplasticizer. Also, results show that self-cured specimen cured with PEG has greater acid resistance as compared to the conventionally cured one.
Association between Nocturnal Enuresis and Decreased Body Height in Chinese School Children
Authors: Ma Jun , Mao Yi , Zhong Shaogen , Zhang Anyi , Zheng Xiangyu , Li Shenghui , Jin Xingming 
Published: April 2022

Abstract
Purpose: Nocturnal enuresis (NE) occurs in 4-18% of children aged ≥5 years, and is associated with sleep disorders, behavioral problems and impaired intellectual development. Whether NE is associated with delayed physical development remains unknown. The present work aimed to assess NE’s association with height in children. Methods: The current cross-sectional trial utilized data generated in a large-sample sleep survey. The final analysis included 21,258 children. Demographic and clinical data, including the frequency of bed-wetting, height and weight, were extracted from the database. Multivariate linear regression analysis, after adjustment for sex, age, ethnicity, sleep time, activity level, method of delivery and maternal age, was carried out to analyze the difference in height between children with NE and healthy controls for various age groups (5–6, 7–8, 9–10 and 11–12 years old). Factors independently predicting NE were identified by multivariable analysis. Results: The NE group had reduced height, higher proportion of males, lower activity level, lower household income and greater use of light during sleep in comparison with the control group. Height difference between NE cases and healthy controls increased with age, reaching 2-3 cm at 11-12 years of age, and was greater in girls than in boys aged up to 10 years. Conclusions: The negative effect of NE on children's height increases with age and is more pronounced in girls than in boys. Early treatment of NE might limit its detrimental effects on children's growth.
Investigation of Synthesis of Biogenic Hematite Nanocubes for environmental Assessment and its innovative Applications associated with Antibacterial, Antifungal, and Antioxidant activity
Authors: Das Debasmita , Ali Selim , Rajbanshi Biplab , Ray Samapika , Barman Sanjoy , Chouhan Divya , Haydar Salman , Mandal Palash , Roy Kanak , Roy Mahendra 
Published: April 2022

Abstract
Nanotechnology is a remarkable platform for water treatment and mitigation from microorganisms and organic dyes when compared to traditional treatment methods. This research work provides the solution to water pollution by magnetic nanoparticles (NPs) through a Fenton-like reaction at neutral pH and also the reusability of wastewater for wheat seeds growth. Benchmarking of obtained results revealed that the synthesized Fe2O3 nanoparticles (IONPs) degrade methylene blue (MB) dye only in 10 min (k1= 0.3387 min-1, R2 = 0.97 using Langmuir-Hinshelwood model and optimum conditions: 20 mM H2O2, pH 7, 0.22 g/L nanocatalyst, MB=10 mg/L) under dark condition. Fruit industry byproducts can be served as a reliable source of green capping and reducing agents for the synthesis of bio-inspired NPs. So, this unique study reports the synthesis of Fe2O3 NPs from lemon peel extract (LPE). The bio-inspired nanoparticles were characterized by Dynamic light scattering (DLS), UV-Visible spectroscopy, Zeta potential, and X-ray diffractometry (XRD).FESEM images clearly claimed for cubical nanostructure ranges from 40-70 nm, which is further confessed by XRD analysis (42 nm). DLS and Zeta potential described the stability of NPs. FTIR spectroscopy describes the interaction of NPs with different organic compounds of LPE. Antibacterial activity was investigated against gram-positive (Bacillus megaterium, Bacillus subtilis) and gram-negative (Escherichia coli, Salmonella typhimurium) aerobics, gram-positive cocci (Staphylococcus aureus) and antifungal activity were performed against Fusarium solani. Biosynthesized NPs show dose-dependent free radical scavenging activity with DPPH.
Tribological Behaviour Study of Zirconia Toughened Alumina Bio-inert Biomaterial Used in Total Hip Joint Implant
Authors: V SHARANRAJ , Ramesha C. M. , Kumar T. Anil , S Abhilash , Kumar Vasantha , R Chandan B , M Sadashiva 
Published: April 2022

Abstract
Zirconia Toughened Alumina (ZTA) is an advanced ceramic bio-inert biomaterial comprising of 80% Alumina and 20% Yttria stabilised Zirconia. These bio-inert biomaterial is used in the field of medical applications specifically in total hip joint due to its unique characteristics of minimum interaction with surrounding tissue and good compatibility. This paper presents the Triboligical behaviour or Wear behaviour of ZTA pin and ZTA Plate when subjected to wear test. Ducom Linear Reciprocating Tribometer was used for testing the wear in ZTA Pin and ZTA Plate specimens. The test results confirmed that the average wear rate of ZTA pin and ZTA plate specimen was minimum as 6.2498x10-9 g/Nm. The coefficient of friction of ZTA pin on ZTA plate was found to be 0.333, frictional force was found to be 6.666 N and operating temperature was 37 ± 0.2o C. The above parameters were within the admissible limits as per ASTM standards used for implant material. Hence Zirconia toughened Alumina Bio-inert material is agood candidate implant biomaterial for Total hip joint replacement and other medical applications.