bluuebunny/crossref_metadata_2025_split huggingface dataset:
Obstructive Sleep Apnoea in Pregnant Women with Chronic Hypertension: A Retrospective Study
Authors: Wang Xiaoyi , Wang Xuexin , Gao Hong 
Published: November 2022

Abstract
Objectives: The primary objective of this study was to determine the incidence of obstructive sleep apnoea (OSA) in pregnant women with chronic hypertension. The secondary objectives were to define the risk factors and assess the maternal-foetal consequences in this population.
Methods: This was a single-centre, retrospective study. The participants were pregnant women with chronic hypertension in the Department of Internal Medicine of Beijing Obstetrics and Gynaecology Hospital, Capital Medical University, between January 2019 and November 2020. Overnight polysomnography (PSG) was performed to diagnose OSA. A total of 99 pregnant women with chronic hypertension who underwent PSG for the first time were included. We reviewed the medical records and collected baseline data, obstetrics, and neonatal information. IBM SPSS Statistics version 25.0 was used for data analysis.
Results: Of the 99 women with chronic hypertension, 63 (63.6%) were diagnosed with OSA, including 41 with mild OSA, 14 with moderate OSA, and eight with severe OSA. Comparing the two groups of chronic hypertensive pregnant women with OSA and those without OSA, the OSA group had higher mean pre-pregnancy body mass index (BMI, 30.68±5.19 vs 27.11±5.22, P=0.001), higher rate of gestational diabetes mellitus (GDM, 38.1% vs 13.9%, P=0.011), a higher induction rate (33.3% vs 11.1%, P=0.014), higher vaginal delivery rate (33.9% vs 13.3%, P=0.034), and a lower caesarean section rate (86.1% vs 66.7%, P=0.034). No significant differences were found in the other evaluated indicators.
Conclusion: The incidence of OSA in pregnant women with chronic hypertension was high in this study. A higher pre-pregnancy BMI is a risk factor for OSA in this population. Pregnant women with chronic hypertension and OSA had a higher risk of developing GDM but a lower rate of caesarean section.
1-nm linewidth room temperature single-photon source from optical microcavity-embedded CsPbI3 perovskite quantum dots
Authors: Farrow Tristan , Dhawan Amit , Marshall Ashley , Ghorbal Alexander , Son Wonmin , Snaith Henry , Smith Jason , Taylor Robert 
Published: November 2022

Abstract
Ultra-narrow bandwidth single-photon sources operating at room temperature are of vital importance for viable optical quantum technologies at scale, including quantum key distribution, cloud-based quantum information processing networks, and quantum metrology. Here we show a room-temperature single-photon source generating polarised photons at a rate of 5MHz based on an inorganic CsPbI3 perovskite quantum dot embedded in a tunable open optical microcavity. When coupled to the mode of the optical cavity, the quantum dot room-temperature emission becomes single-mode and the spectrum narrows down to just ∼ 1 nm. The excellent directionality of the optical modes enables high single-mode collection at RT, offering promising performance for photonic and quantum technology applications. We measure 94% single-photon purity under pulsed and continuous-wave (CW) excitation.
Job preference of preventive medicine students during COVID-19 pandemic,a Discrete Choice Experiment Survey in eastern China
Authors: tian zhuang , zhai min , li hongmin 
Published: November 2022

Abstract
Background: Public health workers are a crucial part of the health workforce especially during COVID-19 pandemic and play an important role in achieving universal health coverage and sustainable development goals. In China, public health human resources are in short supply, the distribution is unequal and the turnover rate is high. DCE was applied to investigate preventive medicine students’ preferred job choice criteria and to investigate the trends in the trade-offs by calculating the marginal rate of substitution between these criteria. This study identified the properties of the jobs primarily selected by preventive students, and aims to estimate the monetary value of each attribute.
Methods: Based on discussions and in-depth interviews with preventive medicine students’ focus groups and a literature review, we created a discrete choice experiment (DCE) that assessed how students’ stated preference for a certain choice was influenced by various job attributes: location, salary, bianzhi, career development opportunities, working environment and workload. We applied this DCE to preventive medicine students in eastern China using a brief structured questionnaire, conditional logit models were used to estimate the utility of each job’s attributes. Willingness to pay (WTP) was estimated as the ratio of the value of the coefficient of interest to the negative of the cost attribute.
Results:In total, 307 respondents completed the questionnaire, and 261 respondents who passed the internal consistency test were analyzed. All attributes were statistically significant. Career development opportunities and work location were most important to respondents. There was preference heterogeneity among respondents, e.g.,3-year medical education college’s students placed higher value on jobs with bianzhi compared to 5-year medical education college’s students. Furthermore, rural students’ willingness to pay is much lower than urban students.
Conclusions: The heterogeneity of attributes showed the complexity of job preferences. Both monetary and nonmonetary job characteristics significantly influenced the job preferences of preventive students in China. A more effective policy intervention to attract graduates to work in rural areas should consider both incentives on the job itself and the background of preventive school graduates.
Toxicity of acute or repeated exposures to particles from pyrotechnic smokes in normal human bronchial epithelial (NHBE) cells 3D culture
Authors: de Lagarde Violaine Martin , Chevalier Laurence , Méausoone Clémence , Cazier Fabrice , Dewaele Dorothée , Cazier-Dennin Francine , Janona Marion , Logie Cathy , Achard Sophie , André Véronique , Rogez-Florent Tiphaine , Monteil Christelle , Corbière Cécile 
Published: November 2022

Abstract
Background
Hexachloroethane (HC) based smokes and colored pyrotechnic smokes are widely used in the military field, as screening and signaling smokes, that are reported to cause pulmonary toxic effects in humans and in animal studies. Toxicity of pyrotechnic smokes might be caused by the inhalation of particles that composed the smoke and gives the desired pyrotechnic effect. In a previous study, we showed that smoke particles from a red signaling smoke (RSS) and from an HC obscuring smoke (HC-OS) have an intrinsic oxidative potential and induce an adaptive response in human small airway epithelial cells after an acute exposure. Thus, the aim of this study was to further explore the underlying mechanisms of toxicity linked to oxidative stress response of RSS and HC-OS particles, by using a 3D model of normal human bronchial epithelial cells (NHBE) cultured at the air-liquid interface (ALI).
Results
Acute exposure (24 h) to HC-OS particles induced a weak antioxidant response, characterized by increases of glutathione level and weak increase of one enzymatic antioxidant mRNA expression (NQO-1). Acute exposure to RSS particles induced oxidative stress characterized by production of reactive oxygen species (ROS), increases of glutathione level, increases mRNA expression of several enzymatic antioxidants (SOD-1, SOD-2, HO-1, NQO-1) and expression of NQO-1 protein as well as increase expression of IL-8 mRNA. We noticed that 24 h post-exposure to RSS particles, antioxidant response was still induced. Additionally, 24 h post-exposure to RSS particles revealed internalization of particles and morphological changes in 3D NHBE cells like loss of cilia and a cubic epithelium. Repeated exposures to RSS particles on 3D NHBE cells did not induce oxidative stress while cubic changing aspects of 3D NHBE cells were observed.
Conclusions
Thus, although further studies are needed to understand the mechanisms underlying these cubic changes, these results revealed differences in toxicity responses between the two types of particles, characterized by an RSS particles-induced oxidative stress as well as morphologic changes. Overall, this study provides a better overview of the toxic effects of pyrotechnic smoke particles whose toxic risks were very little studied.
Epidemiology and molecular characteristics of respiratory syncytial virus (RSV) among Italian community-dwelling adults, 2021/22 season
Authors: Panatto Donatella , Domnich Alexander , Lai Piero Luigi , Ogliastro Matilde , Bruzzone Bianca , Galli Cristina , Stefanelli Federica , Pariani Elena , Orsi Andrea , Icardi Giancarlo 
Published: November 2022

Abstract
Background: Respiratory syncytial virus (RSV) is a leading cause of acute respiratory infections worldwide. While historically RSV research has been focused on children, data on RSV infection in adults are limited. The goal of this study was to establish the prevalence of RSV in Italian adults and analyze its genetic variability during the 2021/22 winter season.
Methods: In this cross-sectional study, a random sample of naso-/oropharyngeal specimens from symptomatic adults seeking for SARS-CoV-2 molecular testing between December 2021 and March 2022 were tested for RSV and other respiratory pathogens by means of reverse-transcription polymerase chain reaction. RSV-positive samples were further molecularly characterized by sequence analysis.
Results: Of 1,213 samples tested, 1.6% (95% CI: 0.9–2.4%) were positive for RSV and subgroups A (44.4%) and B (55.6%) were identified in similar proportions. The epidemic peak occurred in December 2021, when the RSV prevalence was as high as 4.6% (95% CI: 2.2–8.3%). The prevalence of RSV detection was similar (p = 0.64) to that of influenza virus (1.9%). All RSV A and B strains belonged to the ON1 and BA genotypes, respectively. Most (72.2%) RSV-positive samples were also positive for other pathogens being SARS-CoV-2, Pneumococcus pneumoniae and rhinovirus the most frequent. RSV load was significantly higher among mono-infections than co-infections.
Conclusions: During the 2021/22 winter season, characterized by the predominant circulation of SARS-CoV-2 and some non-specific containment measures still in place, a substantial proportion of Italian adults tested positive for genetically diversified strains of both RSV subtypes. In view of the upcoming registration of vaccines, establishment of the National RSV surveillance system is urgently needed.
Identifying important microbial and genetic biomarkers for differentiating right- versus left-sided colorectal cancer using random forest models
Authors: Kolisnik Tyler , Sulit Arielle Kae , Schmeier Sebastian , Frizelle Frank , Purcell Rachel , Smith Adam , Silander Olin 
Published: November 2022

Abstract

Background:Colorectal cancer (CRC) is a heterogeneous disease, with subtypes that have different clinical behaviours and subsequent prognoses. There is a growing body of evidence suggesting that right-sided colorectal cancer (RCC) and left-sided colorectal cancer (LCC) also differ in treatment success and patient outcomes. Biomarkers that differentiate between RCC and LCC are not well-established. Here, we apply random forest (RF) machine learning methods to identify genomic or microbial biomarkers that differentiate RCC and LCC.Methods:RNA-seq expression data for 58,677 coding and non-coding human genes and count data for 28,557 human unmapped reads were obtained from 308 patient CRC tumour samples. We created three RF models for datasets of human genes-only, microbes-only, and genes-and-microbes combined. We used a permutation test to identify features of significant importance. Finally, we used differential expression (DE) and paired Wilcoxon-rank sum tests to associate features with a particular side.Results:RF model accuracy scores were 90%, 70%, and 87% with area under the curve values (AUC) of 0.9, 0.76, and 0.89 for the human genomic, microbial, and combined feature sets, respectively. 15 features were identified as significant in the model of genes-only, 54 microbes in the model of microbes-only, and 28 genes and 18 microbes in the model with genes-and-microbes combined.PRAC1expression was the most important feature for differentiating RCC and LCC in the genes-only model, withHOXB13,SPAG16,HOXC4, andRNLSalso playing a role.Ruminococcus gnavusandClostridium acetireducenswere the most important in the microbial-only model.MYOM3,HOXC4,Coprococcus eutactus,PRAC1, lncRNA AC012531.25,Ruminococcus gnavus,RNLS,HOXC6,SPAG16andFusobacterium nucleatumwere most important in the combined model.Conclusions:Many of the identified genes and microbes among all models have previously established associations with CRC. However, the ability of RF models to account for inter-feature relationships within the underlying decision trees may yield a more sensitive and biologically interconnected set of genomic and microbial biomarkers.
A novel molecular imaging probe 99m Tc-HYNIC-FAPI targeting cancer- associated fibroblasts
Authors: Jiang Yanghongyan , Tian Yaxin , Feng Bei , Zhao Tingting , Du Liang , Yu Xiaodong , Zhao Qian 
Published: November 2022

Abstract
Background
Fibroblast activation protein (FAP) is higher expressed on cancer-associated fibroblasts (CAFs) in most malignant epithelial neoplasms, which is lower expressed in normal tissues. As a promising small molecular probe, FAP inhibitor (FAPI) shows the specific binding to FAP. This study aimed to explore a novel molecular probe 99mTc-HYNIC-FAPI targeting CAFs. The in vitro characteristics of the probe were also evaluated.
Methods
The FAPI targeting to FAP was designed, synthesized and conjugated with the chelator 6-hidrazinylnicotinic acid (HYNIC) for radiolabeling with 99mTc. The radiolabeling yield, radiochemical purity and stability were evaluated by Instant thin-layer chromatography (ITLC) and High performance liquid chromatography (HPLC). The binding and migration ability of the probe were assessed using the tumour cell line.
Results
The radiolabeling yield of 99mTc-HYNIC-FAPI were (97.29 ± 0.46) %. The radiochemical purity was more than 90% and keep stable until 6 h. In vitro experiments, the results indicated that the probe showed binding properties, and inhibited the migration ability of tumor cells.
Conclusion
The novel 99mTc-HYNIC-FAPI probe was successfully radiosynthesized and exhibited good radiochemical purity, stability and in vitro biding ability to tumor cells. The 99mTc-HYNIC-FAPI will be a promising SPECT/CT imaging probe.
Complete Chloroplast Genome of Shorea macrophylla (Engkabang): Structural Features, Comparative and Phylogenetic Analysis
Authors: Chew Ivy Yee Yen , Chung Hung Hui , Lim Leonard Whye Kit , Lau Melinda Mei Lin , Gan Han Ming , Wee Boon Siong , Sim Siong Fong 
Published: November 2022

Abstract

Shorea macrophylla belongs to the Shorea genus under the Dipterocarpaceae family. It is a woody tree that grows in the rainforest in Southeast Asia. The complete chloroplast (cp) genome sequence of S. macrophylla is reported here. The genomic size of S. macrophylla is 150,778 bp and it possesses a circular structure with conserved constitute regions of large single copy (LSC, 83,681 bp) and small single copy (SSC, 19,813 bp) regions, as well as a pair of inverted repeats with a length of 23,642 bp. It has 112 unique genes, including 78 protein-coding genes, 30 tRNA genes, and four rRNA genes. The genome exhibits a similar GC content, gene order, structure, and codon usage when compared to previously reported chloroplast genomes from other plant species. The chloroplast genome of S. macrophylla contained 262 SSRs, the most prevalent of which was A/T, followed by AAT/ATT. Furthermore, the sequences contain 43 long repeat sequences, practically most of them are forward or palindrome type long repeats. The genome structure of S. macrophylla was compared to the genomic structures of closely related species from the same family, and eight mutational hotspots were discovered. The phylogenetic analysis demonstrated a close relationship between Shorea and Parashorea species, indicating that Shorea is not monophyletic. The complete chloroplast genome sequence analysis of S. macrophylla reported in this paper will contribute to further studies in molecular identification, genetic diversity, and phylogenetic research.
Periventricular diffusion gradient of normal-appearing white matter and its transcriptional signatures in normal aging and multiple neurological diseases
Authors: Zhuo Zhizheng , Tian Decai , Duan Yunyun , Li Runzhi , Bai Yutong , Shi Yulu , Xu Xiaolu , Xu Siyao , Lv Shan , Cao Guanmei , Hu Geli , Xu Jun , Zhang Jianguo , Shi Fu-Dong , Chard Declan , Barkhof Frederik , Haller Sven , Zhang Xinghu , Liu Yaou 
Published: November 2022

Abstract
Abnormal periventricular gradient of normal-appearing white matter (NAWM) is a common WM signature of aging and multiple neurological diseases. Here, we investigated the periventricular gradient of NAWM and its association with brain-wide gene expression in middle-aged and elderly participants with Alzheimer's disease, Parkinson's disease, cerebral small vessel disease, and multiple sclerosis compared to young healthy controls (HC), using diffusion magnetic resonance imaging data. Using the normalized neurite density and orientation dispersion indices, we observed the abnormal periventricular diffusion gradients in elderly HC and those with diseases, and the gradients correlated with brain volumes and cognitive performances. Using gene expression data, we observed the periventricular diffusion gradient in a normal aging population and patients with multiple neurological disorders associated with transcriptional changes in endothelial, synaptic, and immunologic functions. We determined a periventricular diffusion gradient of NAWM in normal aging and multiple neurological diseases and revealed its possible transcriptional signatures.
Transcriptome profiling in ovarian cancer cells treated with platelets reveals that TGFBI as a novel prognostic indicator
Authors: Wang Hao , Xu Yin-hai , Guo Yi 
Published: November 2022

Abstract
Background
Ovarian cancer is a gynecologic malignancy with poor prognosis. Present prognostic models for ovarian cancer focus on clinico-pathological parameters, quantifiable prognostic biomarkers at molecular level are urgently needed. Platelets contribute to ovarian cancer progression, thus we aimed to search for new predictors in platelet-treated ovarian cancer cells.
Methods
Microarrays analysis was done with platelet-treated SKOV3 cells and controls (4 replicates in each group). Studies on ovarian cancer cells co-incubated with platelets were searched in the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified by R language. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment were conducted using online software Metascape. Venn diagram was generated to present common DEGs. Candidate genes were determined by protein-protein interaction (PPI) network, Cox proportional hazards model and Kaplan-Meier analysis. The functions of candidate genes were predicted using data from TCGA by R software, and validated by in vitro experiments.
Results
One dataset (GSE155546) met the inclusion criteria and were analyzed with our microarray data. A total of 4553 mRNAs were differentially expressed between the two groups from our own data, whereas 260 genes exhibited significantly differential expression in GSE155546. DEGs involved in extracellular matrix (ECM) organization and system development were found in both datasets. There were 88 overlapping genes between the two datasets. TGFBI was proved to be an independent adverse factor for ovarian cancer. In addition, high expression of AFT3 and CXCL1 showed worse prognosis in ovarian cancer, while IGFBP7 behaved as a protective predictor. Only increased expression of TGFBI led to significant decrease of overall survival (OS), progression-free survival (PFS) and post-progression survival (PPS), therefore TGFBI was selected as the candidate gene. Functionally, TGFBI was predicted to be significantly correlated with epithelial mesenchymal transition (EMT) markers, degradation of ECM, collagen formation and ECM-related genes. In vitro experiments demonstrated that TGFBI could affect the migration and invasiveness of ovarian cancer cells by regulation E-cadherin, Vimentin, N-cadherin and MMP2.
Conclusion
We found TGFBI as a novel prognostic indicator using platelet-treated ovarian cancer model. Functionally, TGFBI could promote ovarian cancer progression by EMT induction and ECM remodeling.
Understanding the Impact of Composition on the Geometry, Electronic Structure and Spectroscopic Profile of an Atomically Precise Gold-Phosphine Cluster
Authors: Pollard Nia , Clayborne Andre 
Published: November 2022

Abstract
Atomically precise nanochemistry relies on understanding the structure and properties of clusters with precise details at the atomic level. Here, we report on the effects of changing the atomic composition of the superatomic gold-gallium cluster [(PL3)8Au9MX2]2+ (L = Ph, Me, H; M = Al, Ga, In; X = F, Cl, Br) using density functional theory (DFT) based methods. By reducing the ligand from PPh3 to PMe3, the geometry illustrates no significant change in the symmetry of the gold core. Similarly, a change in post-transition metal atom or halide does not affect the structure. However, the change in post-transition metal atom does illustrate both blue and red shifts in the spectra by substituting the Al and In for Ga, respectively. The lowest unoccupied molecular orbital and HOMO-LUMO gap decreases as one substitutes the post-transition metal and halide atoms down the periodic table. These studies show the potential for incorporating other post-transition metal and halide atoms in atomically precise gold clusters.