bluuebunny/crossref_metadata_2025_split huggingface dataset:
The PER3 rs772027021 SNP induces pigmentation phenotypes of dyschromatosis universalis hereditaria
Authors: Chen Hongyu , Yang Pingping , Yang Dan , Wang Dongsheng , Lu Mao , Li Yadong , Zhong Zhiqiang , Zhang Jing , Zeng Zhen , Liu Zhi , Zeng Xiaohua , Jia Xu , Xing Qinghe , Zhou Ding'an 
Published: September 2022

Abstract
Dyschromatosis universalis hereditaria (DUH) is a pigmentary genodermatosis characterized by a mixture of hyperpigmented and hypopigmented macules distributed randomly over the body. Although Sterile Alpha motif- and SH3 domain-containing protein 1 ( SASH1 ) and ATP-binding cassette subfamily B, member 6 ( ABCB6 ) have been identified as causative genes for this disorder, some cases involve unknown pathogenic genes. In this study, whole-exome sequencing, data analysis and Sanger sequencing were utilized for a four-generation extended Chinese family with DUH. A single-nucleotide polymorphism (SNP) (c. 517C>T (p. P173S ), rs772027021) variant in exon 5 of Period Circadian Regulator 3 ( PER3 ) (NM_001289861) was detected in each affected individual of the DUH family; the c. 517C>T SNP of PER3 ( PER3 rs772027021 SNP) and a novel mutation in exon 14 of SASH1 (c. 1574C>G (p.T525R)) were both found in the proband. The affected individuals carrying PER3 rs772027021 SNP in this family demonstrated mild pigmented phenotypes compared to those of the proband carrying PER3 rs772027021 SNP and SASH1 T525R mutation. Increased melanin synthesis was induced by PER3 rs772027021 SNP in the melanocytes of affected epithelial tissues. Mutated SASH1 or PER3 rs772027021 SNP alone or cooperation of mutation of SASH1 and PER3 rs772027021 SNP synergistically led to increased melanin synthesis and enhanced proliferation of melanoma cells in vitro. We also phenotypically characterized a commercially available zebrafish mutant line harboring the PER3 rs772027021 SNP to induce melanocyte proliferation in vivo . Our results are the first to reveal that this PER3 SNP may be pathogenic for a novel DUH subtype with mild hyperpigmented and/or hypopigmented phenotypes and that mutation of SASH1 and PER3 cooperatively promotes hyperpigmentation phenotypes.
Machine Learning Prediction Models for Different Stages of Non-small Cell Lung Cancer Based on Tongue and Tumor Marker
Authors: Shi Yu-lin , Wang Hao , Yao Xing-hua , Li Jun , Liu Jia-yi , Chen Yuan , Liu Ling-shuang , Xu Jia-tuo 
Published: September 2022

Abstract
Objective
To analyze the tongue feature of NSCLC at different stages, as well as the correlation between tongue feature and tumor marker, and investigate the feasibility of establishing prediction models for NSCLC at different stages based on tongue feature and tumor marker.
Methods
Tongue images were collected from non-advanced NSCLC patients (n = 109) and advanced NSCLC patients (n = 110), analyzed the tongue images to obtain tongue feature, and analyzed the correlation between tongue feature and tumor marker in different stages of NSCLC. On this basis, five classifiers, logistic regression, SVM, random forest, naive bayes, and neural network, were used to establish prediction models for different stages of NSCLC based on tongue feature and tumor marker.
Results
There were statistically significant differences in tongue feature between the non-advanced and advanced NSCLC groups. The number of index with statistically significant correlations between tongue feature and tumor marker in the advanced NSCLC group was significantly higher than in the non-advanced NSCLC group, and the correlations were stronger. The classifier of neural network based on the tongue feature & tumor marker & baseline data can well predict NSCLC at different stages, the accuracy rates of the five classifiers neural network, random forest, logistic regression, SVM, and naive bayes were 79.69%, 75.00%, 72.81%, 74.06%, 76.56%, and the ROCs were 0.8639, 0.8325, 0.8147, 0.8127, and 0.7969, respectively.
Conclusions
There were statistically significant differences in tongue feature between different stages of NSCLC, with advanced NSCLC tongue feature being more closely correlated with tumor marker. To some extent, tongue feature, tumor marker, and baseline data could be combined to predict NSCLC at different stages. This study established a new methodological reference for the diagnosis of NSCLC at different stages, but more research with a larger sample size was still required in the future.
Rapid declines in lionfish catches in the Saba Bank lobster and snapper trap fisheries, Dutch Caribbean
Authors: Debrot Adolphe , Brunel Thomas , Izioka Ayumi Kuramae 
Published: September 2022

Abstract
Since its introduction in the Western Atlantic more than 30 years ago, the lionfish (Pterois volitans/miles complex) has spread throughout the Gulf of Mexico and the Caribbean having massive and unprecedented ecological impacts. This invasion is among the most studied marine fish invasions but very little is still really known about the population dynamics of the species and the factors ultimately governing its abundance. We here document a large population crash for the lionfish following its rapid increase in abundance on the Saba Bank following its appearance in 2010. In doing so we document the third case of apparent local population boom-bust event for the Greater Caribbean, and the first for the Eastern Caribbean. We also document gradual increases in the mean size of lionfish of the Saba Bank that coincided with the increase and subsequent decline in lionfish abundance. Contrary to the previously documented epizootic disease outbreak associated with the population crash observed in the Gulf of Mexico we were unable to find any signs of the occurrence of epizootic disease. We suggest that the population crash on the Saba Bank might have been due to non-local causes, such as reduced reproductive output in distant larval source populations. Boom-bust dynamics are often witnessed in biological invasions and have critical implications for both understanding and managing invasive species. While the underlying cause for the boom-bust event we document remains unknown, our work helps improve our understanding of this most serious biological invasion.
Secure Spatial Modulation Based on Two-demisonal Generalized Weighted Fractional Fourier Transform Encryption
Authors: Huang Yongxin , Sha Xuejun , Fang Xiaojie , Song Ge 
Published: September 2022

Abstract
In this paper, a two-dimensional generalized weighted fractional Fourier transform (2DGWFRFT) and constellation scrambling (CS) based secure spatial modulation (SM) scheme, called 2DGWFRFT-CS-SM, is proposed to enhance the physical layer security (PLS) of the wireless communication system. The proposed scheme is executed by two steps. In the first step, 2DGWFRFT is implemented as the security kernel for PLS provision. In the second step the parameters of 2DGWFRFT, regarded as the encryption core, control the antenna number rotation to further enhance the security of the SM modulation symbol. Both the 2DGWFRFT signal generation strategy and the SM system including the transfer process have been elaborated to depict the security mechanism of the proposed scheme. Moreover, we give a key extraction algorithm utilized to generate the parameters, which can help scramble the antenna serial number. From the perspective of signal characteristics, the uniqueness of variation in the constellation has been investigated to outperform other cryptography-based encryption algorithms. Finally, the secrecy rates and the energy efficiency of our proposed scheme are theoretically analyzed and evaluated via Monte Carlo simulations, demonstrating that the proposed scheme can achieve a much higher secrecy capacity than artificial noise schemes without requiring additional jamming power consumption and myriad costs of hardware.
Hyaluronic acid injection to coronary sulcus of the penis for the treatment of premature ejaculation
Authors: Kewei CHEN , LI Qing , Xu Tao , Zhang Xiaowei 
Published: September 2022

Abstract
Hyaluronic acid (HA) injection has become a burgeoning method to treat premature ejaculation (PE). In this study, we proposed a modified technique: injecting hyaluronic acid around coronary sulcus to treat premature ejaculation with lowered complications. To improve the current injectable technique to decrease the complications of hyaluronic acid injection in penis while achieving similar effects. A total of 85 patients who had HA injection from January 2018 to December 2019 were analyzed retrospectively in our study. 31 patients received injection at glans penis and 54 patients received injection around coronary sulcus. Intravaginal ejaculation latency time (IELT) was mainly measured to estimate the efficacy and the severity of complications was assessed between two groups. The mean intravaginal ejaculation latency time increased from 123.0±37.28s to 483.5±123.4s at 1 month after intervention, and dropped to 332.7±78.50s and 278.8±67.67s at 3-month follow-up and 6-month follow-up, respectively. In the group of injecting at glans penis, the incidence of complications is 25.8%; it is 1.9% in the group of injecting around coronary sulcus. No severe complication was reported in both groups. modified technique of injecting around coronary sulcus decreases complications and it has the potential to become a new injectable technique for treating premature ejaculation.
Effect of past Chlamydophila pneumoniae infection on the clinical course of COVID-19: A retrospective cohort study
Authors: Horiuchi Hiroshi , Utada Shusuke , Shinomiya Yoshie , Sogo Azusa , Miyagawa Takao , Niida Shoko , Okano Hiromu , Suzuki Naoya , Otsuka Tsuyoshi , Miyazaki Hiroshi , Furuya Ryosuke 
Published: September 2022

Abstract
Background
Although Chlamydophila pneumoniae (CP) is known to play a role in atherosclerosis and endothelial injury, its effect on the clinical course of coronavirus disease 2019 (COVID-19), which was also reported to be a vascular disease, remains unknown.
Methods
In this retrospective cohort study, we examined 76 COVID-19 patients and 44 bacterial pneumonia patients who visited a tertiary emergency center in Japan between April 1, 2021, and March 31, 2022. CP antibody levels, including IgM, IgG, and IgA, were measured.
Results
Among all patients, the CP IgA-positive rate was significantly associated with age (P = 0.002). Between the COVID-19 and non-COVID-19 groups, no difference in the positive rate for both CP IgG and IgA was observed (P = 1.00, and 0.51, respectively). However, in univariate analysis, age, proportion of males, and mortality were significantly higher in the IgA-positive group than in the IgA-negative group (60.35 vs. 76.33, P = 0.001; 59.6 vs. 95.2, P = 0.002; 11.5 vs. 33.3, P = 0.042, respectively). Age-adjusted and sex-adjusted logistic regression analyses revealed that IgA positivity was not significantly associated with higher mortality (odds ratio [OR], 0.36; 95% confidence interval [CI], 0.10–1.29; P = 0.12, and OR, 0.38; 95% CI, 0.11–1.30; P = 0.12, respectively).
Conclusion
Persistent infection with CP may be a contributing factor to the higher mortality of elderly male patients with COVID-19.
The impact of migration-related characteristics on the risk of TORCH infections among women of childbearing age: A population-based study in southern China
Authors: Li Rui , Han Lu , Xiong Wenxue , Wang Wenjuan , Fan Chaonan , Li Mingzhen , Liu Xiaohua , Ling Li 
Published: September 2022

Abstract
Background
TORCH infections are the most common prenatal infections causing congenital malformation and infant mortality, especially in developing countries. Migrant women might be vulnerable to TORCH infections, but little is known about the association between migration-related characteristics and TORCH infection risk. This study aimed to investigate the impact of migrant status, migration distance, and the spouse’s migrant status on the TORCH epidemic among women of childbearing age.
Methods
Based on the National Free Preconception Health Examination Project, we analyzed a representative dataset of TORCH infections among women of childbearing age (15–49 years old) in Guangdong Province of China (2014–2019, n = 2,451,297). The past and/or recent infection status of TORCH infections (Toxoplasma gondii [TOX], Cytomegalovirus [CMV], and Rubella virus [RV]) were identified. Demographic and migration-related characteristics were collected. We thoroughly assessed the prevalence of TORCH infections in both migrant and native women and estimated adjusted odd ratios (aOR) for migration-related characteristics using multivariable logistic regression after adjusting the other sociodemographic factors.
Results
Among all 2,451,297 participants, 443,725 (18.1%) were migrant women. Migrant women presented a lower risk of past TOX infection (aOR: 0.89, 0.88–0.91) suggesting a healthy migrant effect (HME), but a higher risk of recent TOX infection (aOR: 1.88, 1.77–1.99), past CMV infection (aOR: 1.26, 1.25–1.28) and RV infection in natural ways (aOR: 1.05, 1.04–1.06). Compared with intra-provincial migrants, inter-provincial migrants had a lower past TOX infection (aOR: 0.88, 0.85–0.91), but a higher risk of recent TOX infection (aOR: 1.16, 1.05–1.27) and RV infection (aOR: 1.33, 1.31–1.36). In addition, having a migrant spouse was associated with a higher risk for all types of infection.
Conclusions
This study reported the association of migrant status and migration distance with TORCH infections, although the significance and directionality of these associations varied between pathogens. The spouse’s migrant status further amplified the infection risk for all types of pathogens. Our findings suggested interventions for preventing the spread of CMV and RV infection and new acquisition of TOX infection for migrants in southern China, to narrow the native-migrant health inequity and decrease the incidence of prenatal infections and related adverse outcomes.
The lumbar spinal endplate lesions grades and association with lumbar disc disorders, and lumbar bone mineral density in a healthy Chinese cohort
Authors: Pei Jingzhe , Yu Aihong , Geng Jian , Liu Yandong , Wang Ling , Shi Jia , Zhou Fengyu , Zhang Tianyu , Huang Pengju , Cheng Xiaoguang 
Published: September 2022

Abstract
Background Lumbar vertebral endplates lesions (LEPLs), as one of the etiology of low back pain (LBP) which is one of the most common causes of health care cost, have an unclear pathogenesis. Although it gradually regarded as the focus in recent years, almost all studies have focused on symptomatic people, not healthy people. Therefore, our study was designed to determine the prevalence and distribution patterns of LEPLs and their associations with lumbar disc herniation (LDH), lumbar disc degeneration (LDD), and lumbar vertebral volumetric bone mineral density (vBMD) in a health cohort. Methods Seven hundred and fifty healthy participants aged 20–60 years were enrolled in the study from a study on the degeneration of the spine and knee. In this observational study, a lumbar quantitative computed tomography (QCT) and MRI scan were performed among participants within 48 hours. T2-weighted sagittal lumbar MRI images for all included subjects were identified for LEPLs by two independent observers based on morphological and local characteristics. Lumbar vertebral vBMD was measured with QCT. The age, BMI, waistline, hipline, lumbar vBMD, LDD, and LDH were measured to investigate their associations with LEPLs.Results The prevalence was higher among the male subjects. 80% of endplates were recognition as no lesions with a significant difference between female (75.6%) and male subjects (83.4%) (p < 0.001). The most common lesions were “wavy/irregular” and “notched”, and “fracture” is most involved in L3-4 inferior endplate both in two genders. Lesions were found to be associated with LDH (≥2 levels： OR=6.859, P＜0.001； 1 level： OR=2.328， P=0.002 in men. OR=5.004, P＜0.001； OR=1.805， P= 0.014 in women) reference for non-LDH, and hipline in men(OR=1.123, P＜0.001). Weighted kappas of 0.66 and 0.87 were, respectively, assessed for the inter-and intra-observer reliabilities of the classification system. Conclusions Endplate lesions are also the common findings on lumbar MRIs in general people, particularly in men, and the presence, and advance from slightly to severely could be mainly attributed to LDH and men’s higher hipline.
Bacterial Outer Membrane Vesicle Based Versatile Nanosystem Boosts the Efferocytosis Blockade Triggered Tumor-Specific Immunity
Authors: Zhuang Wan-Ru , Wang Yunfeng , Nie Weidong , Lei Yao , Liang Chao , He Jiaqi , Zuo Liping , Huang Lili , Xie Hai-Yan 
Published: September 2022

Abstract
Efferocytosis inhibition is emerging as an attractive strategy for antitumor immune therapy because of the subsequent leak of abundant immunogenic contents. However, the practical efficacy is seriously impeded by the immunosuppressive tumor microenvironments. Here, we construct a versatile nanosystem that can not only inhibit the efferocytosis but also boost the following antitumor immunity. UNC2025 is loaded into the bacterial outer membrane vesicles (OMVs), which are then modified with maleimide (Mal). The prepared mU@OMVs effectively inhibits the efferocytosis by promoting the uptake while preventing the MerTK phosphorylation of tumor associated macrophages, and then captures the released TAAs through forming universal thioether bonds. The obtained in situ vaccine effectively transfers to LNs with by virtue of the intrinsic features of OMVs, and then provokes intense immune responses that can efficiently prevent the growth, metastasis and recurrence of tumors in mice, providing a novel strategy for cancer immunotherapy.
Trajectories of plant communities in Massachusetts, U.S.A. cranberry farms discontinued from agriculture
Authors: Neill Christopher , Pulak Anastasia , Miller Haley , Hoekstra Benjamin , Klionsky Sarah 
Published: September 2022

Abstract
Retirement of cultivated croplands creates potential for ecosystem and wetland restoration, but vegetation and soil legacies of cropping influence the development of post-agriculture vegetation. In low-lying coastal watersheds of southeastern Massachusetts, cranberries (Vaccinium macrocarpon Aiton) are cultivated in commercial "bogs" largely on diked, leveled and sanded bog beds created from historic wetlands. Current low cranberry prices and expanding cranberry production elsewhere now increase the likelihood of cranberry farmland retirement. We quantified the trajectories of plant species richness and cover, and plant characteristics (life form, native or non-native, wetland or non-wetland) in a chronosequence of cranberry bog beds that spanned from cultivated bog beds to those retired from cropping and revegetated for 90 years with no post-cropping management. Species richness increased from active bog beds to 10-20 year-old bog beds and subsequently decreased. Post-retirement species richness was overwhelmingly dominated by native species. Shrub and tree richness and cover increased steadily over time. The richness of wetland, upland and facultative species all increased quickly after retirement and then declined in the oldest retired bog beds. The basal area and canopy cover of red maple (Acer rubrum) and pitch pine (Pinus rigida) increased over time. Vegetation followed a relatively predictable succession trajectory and the plant community after five to nine decades was predominantly forested and dominated by non-wetland plants. Encouragement of long-term persistence of greater diversity and cover of wetland plant species on retired cranberry farms will likely require active hydrological and soil modifications that decrease sand depth and raise water tables.
Splines finite element solver for one-dimensional time-dependent Maxwell’s equations via Fourier Transform Discretization
Authors: Addam Mohamed , Bouhamidi Abderrahman , BARKANI Imad EL 
Published: September 2022

Abstract
In this article, we solve the time-dependent Maxwell coupled equations in their one-dimensional version relatively to space-variable. We effectuate a variable reduction via Fourier transform to make the time variable as a frequency parameter easy and quickly to manage. A Galerkin variational method based on higher-order spline interpolations is used to approximate the solution relatively to the spacial variable. So, the state of existence of the solution, its uniqueness, and its regularity are studied and proved, and the study is also provided by an error estimate. Also, we use the critical Nyquist frequency to calculate numerically the solution of the Inverse Fourier Transform(IFT); and for all numerical computations, we consider several quadrature methods. We give some experiments to illustrate the success of such an approach. Finally, we apply the higher-order spline interpolants to solve the first kind of Fredholm integral equation and Pocklington’s integro-differential equation to treat the signal reconstruction inside the wire antennas
DFT calculation for graphene/hexagonal-boron-nitride nanoisland applicate in photonic device
Authors: Abed Hussein Hakim , Al-Seady Mohammed A. , Abduljalil Hayder M. , Hassan Saif M. , Hasim Ahmed , Kahaly Mousumi Upadhyay , Mohammed Rajaa 
Published: September 2022

Abstract
In the present study, density function theory (DFT) tool is used to compute structural, electronic and optical properties for hexagonal-boron-nitride (h-BN) nanosheet, perfect and defected (one carbon atom remove) graphene (G)/boron-nitride nanoisland. The basis set 6-31G is utilizing in a present study with hybrid function (B3LYP). Relaxation calculation is showing that all bond length between atoms are agreement with theoretical and experimental measurements, also the surface of all systems was remain in plane form. Energy gap calculation shows that a h-BN nanosheet has insulator nature. Doping h-BN by carbon (C) and defected (by remove one carbon atom) the energy gap is modifying and reduce from (6.006) to (3.464 and 1.569) eV, respectively. In the other hand, doped and defected mechanism enhance the conductivity of h-BN nanosheet. UV-Visible calculation is showing that h-BN absorbing in the ultra-violet region, doped and defected nanoisland were absorbed light in the visible region of electromagnetic radiation and shifted to long wavelength (Low energy). Fourier transformation-infrared radiation (FT-IR) calculations are showing that all active group are appearing like (C-C), (C-B), (C-N), (B-H) and (N-H) and approximately agreement with experimental measurements. Light harvesting efficiency (LHE) calculation is concluding that h-BN/G nanoisland have high ability to harvest light compared with h-BN nanosheet and defected h-BN/G nanoisland. Results show that higher occupied molecular orbital (HOMO) is being distributed above minimum conduction band (CBM) of titanium dioxide (TiO2), and lower unoccupied molecular orbital (LUMO) is being populate below iodine/ tri-iodine (I/I3) electrolyte. Meanwhile, all nanostructure is having ability to generate pair electron-hole. Finally, results show that the measurements are indicating that it is possible to apply the composites under study in the field of photonic devices especially in solar cell field.