bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of an Inverted Synthetic Control Method to Estimate Effects of Recent Drug Overdose Good Samaritan Laws, Overall and by Black/White Race/Ethnicity
Authors: Townsend Tarlise N , Hamilton Leah K , Rivera-Aguirre Ariadne , Davis Corey S , Pamplin John R , Kline David , Rudolph Kara E , Cerdá Magdalena 
Published: July 2022

AbstractOverdose Good Samaritan laws (GSLs) aim to reduce mortality by providing limited legal protections when a bystander to a possible drug overdose summons help. Most research into the impact of these laws is dated or potentially confounded by coenacted naloxone access laws. Lack of awareness and trust in GSL protections, as well as fear of police involvement and legal repercussions, remain key deterrents to help-seeking. These barriers may be unequally distributed by race/ethnicity due to racist policing and drug policies, potentially producing racial/ethnic disparities in the effectiveness of GSLs for reducing overdose mortality. We used 2015–2019 vital statistics data to estimate the effect of recent GSLs on overdose mortality, overall (8 states) and by Black/White race/ethnicity (4 states). Given GSLs’ near ubiquity, few unexposed states were available for comparison. Therefore, we generated an “inverted” synthetic control method (SCM) to compare overdose mortality in new-GSL states with that in states that had GSLs throughout the analytical period. The estimated relationships between GSLs and overdose mortality, both overall and stratified by Black/White race/ethnicity, were consistent with chance. An absence of effect could result from insufficient protection provided by the laws, insufficient awareness of them, and/or reticence to summon help not addressable by legal protections. The inverted SCM may be useful for evaluating other widespread policies.
Comparison of 2 different inhaled epoprostenol dosing strategies for acute respiratory distress syndrome in critically ill adults: Weight-based vs fixed-dose administration
Authors: Buckley Mitchell S , Mendez Angel , Radosevich John J , Agarwal Sumit K , MacLaren Robert 
Published: July 2022

AbstractPurposeInhaled epoprostenol (iEPO) is a viable, temporizing option for acute respiratory distress syndrome (ARDS), although the optimal iEPO dosing strategy remains inconclusive. The purpose of this study was to evaluate oxygenation and ventilation parameters in a comparison of weight-based and fixed-dose iEPO in adult patients with moderate-to-severe ARDS.MethodsA retrospective cohort study was conducted at 2 academic medical centers in adult intensive care unit (ICU) patients administered either fixed-dose or weight-based iEPO for moderate-to-severe ARDS. The primary endpoint was the highest recorded change in the ratio of arterial oxygen partial pressure to fractional inspired oxygen (PaO2/FiO2) within 4 hours of baseline. Secondary analyses compared responder rates within 4 hours of initiation, oxygenation and ventilation parameters, in-hospital mortality rates, mechanical ventilation duration, length of stay (ICU and hospital), and tracheostomy rates between the study groups.ResultsA total of 294 patients were included, n = 194 with 100 (34.0%) and 194 (66.0%) in the weight-based and fixed-dose iEPO groups, respectively. The mean (SD) change in the highest recorded PaO2/FiO2 value from baseline up to 4 hours after initiation in the fixed-dose and weight-based groups was 81.1 (106.0) and 41.0 (72.5) mm Hg, respectively (P = 0.0015). The responder rate at 4 hours after iEPO initiation was significantly higher in the fixed-dose group (69.9%) than in the weight-based group (30.1%) (P = 0.02). The only predictor of response was fixed-dose administration (odds ratio, 3.28; 95% confidence interval, 1.6-6.7; P = 0.0012). Clinical outcomes were comparable between the groups.ConclusionFixed-dose iEPO was associated with significantly higher response rates then weight-based iEPO during the first 4 hours of therapy. Fixed-dose iEPO is a more convenient strategy than weight-based approaches.
Histamine elimination by a coupling reaction of fungal amine oxidase and bacterial aldehyde oxidase
Authors: Usui Masakatsu , Kubota Hikari , Ishihara Mizuki , Matsuki Haruka , Kawabe Shinya , Sugiura Yoshimasa , Kataoka Naoya , Matsushita Kazunobu , Ano Yoshitaka , Akakabe Yoshihiko , Hours Roque A , Yakushi Toshiharu , Adachi Osao 
Published: July 2022

ABSTRACT
Histamine (HIST) and other biogenic amines found in fish and fishery products accumulated by the action of bacterial amino acid decarboxylase cannot be decomposed and eliminated by heating or other chemical methods. A simple method for HIST elimination is proposed by a coupling reaction of the fungal amine oxidase (FAO) and bacterial aldehyde oxidase (ALOX) of acetic acid bacteria. As a model reaction, FAO oxidized benzylamine to benzaldehyde, which in turn was oxidized spontaneously to benzoic acid with ALOX. Likely, in HIST elimination, FAO coupled well with ALOX to produce imidazole 4-acetic acid from HIST with an apparent yield of 100%. Imidazole 4-acetaldehyde was not detected in the reaction mixture. In the absence of ALOX, the coupling reaction was incomplete given a number of unidentified substances in the reaction mixture. The proposed coupling enzymatic method may be highly effective to eliminate toxic amines from fish and fishery products.
A Novel Representation and Prediction Initiative for Underground Water by Using Deep Learning Technique of Remote Sensing Images
Authors: Sureshkumar Veluguri , Somarajadikshitar Rajasomashekar , Beeram B Sarala 
Published: July 2022

Abstract
This paper intends to introduce a novel groundwater prediction model by inducing the novel hydro indices that are not yet popular in earlier techniques. As per the proposed work, statistical features like mean, median, skewness and kurtosis are estimated. Moreover, the vegetation index includes simple ratio, normalized difference vegetation index, Kauth–Thomas Tasseled cap transformation and infrared index transformation. Furthermore, a novel hydro index is formulated by combining the statistical model function with the vegetation index. Subsequently, the detection process is carried out by ensemble technique, which includes the classifiers like random forest (RF), neural network (NN), support vector machine (SVM) and deep belief network (DBN). The final predicted result is attained from DBN. The performance of the adopted model is computed to the existing models with respect to certain measures. At learning rate 50, the maximum accuracy of the proposed model is 45.65, 34.78, 58.70, 72.83, 18.48 and 23.91% better than the existing models like SVM, RF, convolutional neural network, K-nearest neighbors, NN and artificial neural network, respectively.
The Lambeth Articles (1595) and the Doctrinal Stance of the Church of England
Authors: Tyacke Nicholas 
Published: July 2022

Abstract
The Lambeth Articles have always bulked large in debates about the Calvinist tenor of sixteenth-century English Protestantism. But in a recent article, Debora Shuger has raised the stakes dramatically. Not content to deny that the Lambeth Articles were ‘Calvinist’, she claims that they were the work of a group of ‘non-Calvinist divines’, specially commissioned by Archbishop Whitgift to produce a ‘consensus document’ designed to put an end to the predestinarian controversy which had broken out at Cambridge University. Paradoxically, Shuger combines this with the view that the Lambeth Articles were nevertheless suppressed, on the orders of Queen Elizabeth. It follows that the idea of a subsequent ‘rise of English Arminianism’ is fundamentally flawed. The present piece argues that the Lambeth Articles in their final form (a) emanated from a meeting of the Court of High Commission at Lambeth, presided over by Whitgift himself, Bishop Richard Fletcher of London, and Richard Vaughan, the bishop-elect of Bangor, all of them Calvinists; (b) are best understood as speaking the language of sublapsarian Calvinism; and (c) were not suppressed but continued to be regarded as interpretative guidelines until the 1620s. The 1620s, however, witnessed a dramatic renversement when, unlike the 1590s, Calvinism was indeed suppressed by royal command and at the hands of a new breed of anti-Calvinist divines. Nevertheless, via the Irish Articles of 1615, the Lambeth Articles were to attain an afterlife as part of the Westminster Confession of Faith promulgated in 1648.
Premature translation of the Drosophila zygotic genome activator Zelda is not sufficient to precociously activate gene expression
Authors: Larson Elizabeth D , Komori Hideyuki , Fitzpatrick Zoe A , Krabbenhoft Samuel D , Lee Cheng-Yu , Harrison Melissa 
Published: July 2022

Abstract
Following fertilization, the unified germ cells rapidly transition to a totipotent embryo. Maternally deposited mRNAs encode the proteins necessary for this reprogramming as the zygotic genome remains transcriptionally quiescent during the initial stages of development. The transcription factors required to activate the zygotic genome are among these maternally deposited mRNAs and are robustly translated following fertilization. In Drosophila, the mRNA encoding Zelda, the major activator of the zygotic genome, is not translated until 1 h after fertilization. Here we demonstrate that zelda translation is repressed in the early embryo by the TRIM-NHL protein Brain tumor (BRAT). BRAT also regulates Zelda levels in the larval neuroblast lineage. In the embryo, BRAT-mediated translational repression is regulated by the Pan Gu kinase, which is triggered by egg activation. The Pan Gu kinase phosphorylates translational regulators, suggesting that Pan Gu kinase activity alleviates translational repression of zelda by BRAT and coupling translation of zelda with that of other regulators of early embryonic development. Using the premature translation of zelda in embryos lacking BRAT activity, we showed that early translation of a zygotic genome activator is not sufficient to drive precocious gene expression. Instead, Zelda-target genes showed increased expression at the time they are normally activated. We propose that transition through early development requires the integration of multiple processes, including the slowing of the nuclear division cycle and activation of the zygotic genome. These processes are coordinately controlled by Pan Gu kinase-mediated regulation of translation.
The PAICE suite reveals circadian posttranscriptional timing of noncoding RNAs and spliceosome components in Mus musculus macrophages
Authors: Buel Sharleen M , Debopadhaya Shayom , De los Santos Hannah , Edwards Kaelyn M , David Alexandra M , Dao Uyen H , Bennett Kristin P , Hurley Jennifer M 
Published: July 2022

Abstract
Circadian rhythms broadly regulate physiological functions by tuning oscillations in the levels of mRNAs and proteins to the 24-h day/night cycle. Globally assessing which mRNAs and proteins are timed by the clock necessitates accurate recognition of oscillations in RNA and protein data, particularly in large omics data sets. Tools that employ fixed-amplitude models have previously been used to positive effect. However, the recognition of amplitude change in circadian oscillations required a new generation of analytical software to enhance the identification of these oscillations. To address this gap, we created the Pipeline for Amplitude Integration of Circadian Exploration suite. Here, we demonstrate the Pipeline for Amplitude Integration of Circadian Exploration suite’s increased utility to detect circadian trends through the joint modeling of the Mus musculus macrophage transcriptome and proteome. Our enhanced detection confirmed extensive circadian posttranscriptional regulation in macrophages but highlighted that some of the reported discrepancy between mRNA and protein oscillations was due to noise in data. We further applied the Pipeline for Amplitude Integration of Circadian Exploration suite to investigate the circadian timing of noncoding RNAs, documenting extensive circadian timing of long noncoding RNAs and small nuclear RNAs, which control the recognition of mRNA in the spliceosome complex. By tracking oscillating spliceosome complex proteins using the PAICE suite, we noted that the clock broadly regulates the spliceosome, particularly the major spliceosome complex. As most of the above-noted rhythms had damped amplitude changes in their oscillations, this work highlights the importance of the PAICE suite in the thorough enumeration of oscillations in omics-scale datasets.
The genome of Dioscorea zingiberensis sheds light on the biosynthesis, origin and evolution of the medicinally important diosgenin saponins
Authors: Li Yi , Tan Chao , Li Zihao , Guo Jingzhe , Li Song , Chen Xin , Wang Chen , Dai Xiaokang , Yang Huan , Song Wei , Hou Lixiu , Xu Jiali , Tong Ziyu , Xu Anran , Yuan Xincheng , Wang Weipeng , Yang Qingyong , Chen Lingling , Sun Zongyi , Wang Kai , Pan Bo , Chen Jianghua , Bao Yinghua , Liu Faguang , Qi Xiaoquan , Gang David R , Wen Jun , Li Jiaru 
Published: July 2022

Abstract
Diosgenin saponins isolated from Dioscorea species such as D. zingiberensis exhibit a broad spectrum of pharmacological activities. Diosgenin, the aglycone of diosgenin saponins, is an important starting material for the production of steroidal drugs. However, how plants produce diosgenin saponins and the origin and evolution of the diosgenin saponin biosynthetic pathway remain a mystery. Here we report a high-quality, 629-Mb genome of D. zingiberensis anchored on 10 chromosomes with 30 322 protein-coding genes. We reveal that diosgenin is synthesized in leaves (‘source’), then converted into diosgenin saponins, and finally transported to rhizomes (‘sink’) for storage in plants. By evaluating the distribution and evolutionary patterns of diosgenin saponins in Dioscorea species, we find that diosgenin saponin-containing may be an ancestral trait in Dioscorea and is selectively retained. The results of comparative genomic analysis indicate that tandem duplication coupled with a whole-genome duplication event provided key evolutionary resources for the diosgenin saponin biosynthetic pathway in the D. zingiberensis genome. Furthermore, comparative transcriptome and metabolite analysis among 13 Dioscorea species suggests that specific gene expression patterns of pathway genes promote the differential evolution of the diosgenin saponin biosynthetic pathway in Dioscorea species. Our study provides important insights and valuable resources for further understanding the biosynthesis, evolution, and utilization of plant specialized metabolites such as diosgenin saponins.
The compressive study of energy security prospects in India through Solar Power
Authors: Prakash Om , Ahmad Asim , Kumar Anil , Chatterjee Rajeshwari , Sharma Shubham , Alayi Reza , Monfared Hossein 
Published: July 2022

Abstract
Energy security depends upon the supply and demand of electricity. Power supply volatility is a challenge in India power sector. India is mainly dependent on coal, which is not a viable long-term option. Hence the future of energy supply in India is renewable energy, and solar energy is the most prominent and reliable source of renewable energy. The solar power plant is classified as a low, medium, and high temperature based solar power plant. The low- temperature solar power plant, the collectors, are unglazed plates. In contract, medium-temperature collectors are flat plates, and high-temperature collectors concentrate sunlight using mirrors and lenses, achieving temperature and pressure up to 3000 °C and 20 bar. This article discusses the existing total energy scenario, current solar energy developments, supportive solar energy policies, and solar energy prospects. The tactics, availability, perspectives, potential, and successes of solar energy in India are discussed in this study. The authors compared the barriers to solar energy acceptance and examined the topic of social dissimilarity from a broad theoretical perspective. The study offers policymakers at the federal and state levels, as well as investors, a perspective on issues that may lead to investments in this area. The study states that solar energy could meet above 50% of electricity sector demand in India in 2040. This study determined that solar energy incidence in India is around 5000 trillion KWh (kilowatt-hours) each year. The solar energy accessible in a single year surpasses the energy output from the petroleum derivative. The average energy from the solar power plant is 0.30 kWh per m2 equal to the 1400–1800 peak rated capacity. India has many solar power facilities, making it one of the top producers of renewable energy power.
Drop-in assessment of plug-in R404A refrigeration equipment using low-global warming potential mixtures
Authors: Devecioğlu Atilla G , Oruç Vedat 
Published: July 2022

Abstract
This study analyses the performance of plug-in refrigeration equipment, which has the condenser on the system and delivers heat to the indoor medium, using mixtures with low-global warming potential (GWP). The theoretical drop-in replacement of R404A that is widely used in the plug-in refrigeration equipment, is investigated utilizing refrigerants with GWP&lt;150 such as R454C, R455A, R457A and R465A. In the study, four cases of evaporation temperatures (-25°C, -15°C, -5°C and 0°C) and two cases of condenser temperatures (30°C and 40°C) were considered for freezer and chiller modes of the equipment. According to the obtained results, the power consumption of R404A is determined to be higher than other refrigerants for both studied condenser temperatures. The mass flow rate circulating in the system decreases using R404A’s alternatives. Furthermore, the highest COP is reached with R457A which has a greater COP than R404A about by 8.5%. The compressor discharge pressures of alternative refrigerants are smaller compared to R404A as well. The reduction in CO2 emission is also presented in terms of total equivalent warming impact (TEWI) computations. The conducted analysis points out that it will be possible to use directly the investigated mixtures (especially R457A and R455A are most promising ones) in plug-in refrigeration devices as alternatives to R404A without performing any constructional modification in the system.
Prototype Pathogen Approach for Vaccine and Monoclonal Antibody Development: A Critical Component of the NIAID Plan for Pandemic Preparedness
Authors: Cassetti M Cristina , Pierson Theodore C , Patterson L Jean , Bok Karin , DeRocco Amanda J , Deschamps Anne M , Graham Barney S , Erbelding Emily J , Fauci Anthony S 
Published: July 2022

Abstract
Severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1) emerged 20 years ago, presaging a series of subsequent infectious disease epidemics of international concern. The recent emergence of SARS-CoV-2 has underscored the importance of targeted preparedness research to enable rapid countermeasure development during a crisis. In December 2021 the National Institute of Allergy and Infectious Diseases (NIAID), building upon the successful strategies developed during the SARS-CoV-2 response and to prepare for future pandemics, published a pandemic preparedness plan that outlined a research strategy focused on priority pathogens, technology platforms, and prototype pathogens. To accelerate the discovery, development, and evaluation of medical countermeasures against new or previously unknown pathogens of pandemic potential, we present here a strategy of research directed at select prototype pathogens. In this manner, leveraging a prototype pathogen approach may serve as a powerful cornerstone in biomedical research preparedness to protect public health from newly emerging and reemerging infectious diseases.
Sensitivity of Reverse Transcription Polymerase Chain Reaction Tests for Severe Acute Respiratory Syndrome Coronavirus 2 Through Time
Authors: Binny Rachelle N , Priest Patricia , French Nigel P , Parry Matthew , Lustig Audrey , Hendy Shaun C , Maclaren Oliver J , Ridings Kannan M , Steyn Nicholas , Vattiato Giorgia , Plank Michael J 
Published: July 2022

AbstractBackgroundReverse transcription polymerase chain reaction (RT-PCR) tests are the gold standard for detecting recent infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Reverse transcription PCR sensitivity varies over the course of an individual’s infection, related to changes in viral load. Differences in testing methods, and individual-level variables such as age, may also affect sensitivity.MethodsUsing data from New Zealand, we estimate the time-varying sensitivity of SARS-CoV-2 RT-PCR under varying temporal, biological, and demographic factors.ResultsSensitivity peaks 4–5 days postinfection at 92.7% (91.4%–94.0%) and remains over 88% between 5 and 14 days postinfection. After the peak, sensitivity declined more rapidly in vaccinated cases compared with unvaccinated, females compared with males, those aged under 40 compared with over 40s, and Pacific peoples compared with other ethnicities.ConclusionsReverse transcription PCR remains a sensitive technique and has been an effective tool in New Zealand’s border and postborder measures to control coronavirus disease 2019. Our results inform model parameters and decisions concerning routine testing frequency.
Spending Windfall (“Found”) Time on Hedonic versus Utilitarian Activities
Authors: Chung Jaeyeon (Jae) , Lee Leonard , Lehmann Donald R , Tsai Claire I 
Published: July 2022

AbstractConsumers often gain extra free time unexpectedly. Given the increasing time pressure that consumers experience in their daily lives, it is important to understand how they spend windfall (or unexpected) free time, which we term found time. In a series of five laboratory experiments and naturalistic field studies, we found that consumers spend more of their free time on hedonic activities than on utilitarian activities when they gain the time unexpectedly (i.e., found time), but not when they know about the free time in advance. This pattern occurs consistently regardless of whether consumers gain the time from canceled work-related or leisure activities. In addition, our studies uncovered perceived busyness as a ubiquitous yet unexplored moderator for the windfall gain literature: the inclination to allocate found time to hedonic consumption decreases when consumers perceive themselves to be busy at the moment. We discuss several potential accounts for the effect of unexpectedness on time expenditure, including a perceived fit between the nature of found time (a fun windfall gain) and hedonic consumption, need for justification, and planning.
Trace metals at the frontline of pathogen defence responses in non-hyperaccumulating plants
Authors: Morina Filis , Küpper Hendrik 
Published: July 2022

Abstract
Metal hyperaccumulation is an exclusive evolutionary trait contributing to efficient plant defence against biotic stress. The defence can be based on direct metal toxicity or the joint effects of accumulated metal and organic compounds, the latter being based on integrated signalling networks. While the role of metals in biotic stress defence of hyperaccumulators has been intensively studied, their role in the pathogen immunity of non-accumulator plants is far less understood. New findings show that in metal non-hyperaccumulating plants, localized hot spots of zinc, manganese, and iron increase plant immunity, while manipulation of nutrient availability may be used for priming against subsequent pathogen attack. Recent findings on the role of metals in plant–pathogen interactions are discussed considering the narrow line between deficiency and toxicity, host–pathogen nutrient competition and synergistic effects of simultaneous metal and biotic stress. We discuss the suitability of the direct-defence and joint-effects hypotheses in non-hyperaccumulating plants, and the involvement of metals as active centres of immunity-related enzymes. We also consider future challenges in revealing the mechanisms underlying metal-mediated plant immunity.