bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of HIS-based intervention on patient education process and patient satisfaction with nurses' education
Authors: Toulabi Tahereh , Mohammadipour Fatemeh 
Published: June 2022

Abstract
Objective
This quasi-experimental study (before and after intervention) was designed to determine the impact of hospital information system-based intervention on the patient education process and patient satisfaction in cardiac and cardiac intensive care units.

Results
Each nurse was observed at the time of patient education on average on eight shifts (total of 256 shifts), and at last 1350 computerized reports before and after the intervention were analyzed and 150 patient satisfaction with nurses' education questionnaires were completed before and after the intervention.
After the intervention, the patient education scores were significantly improved (p < 0.001). In addition, the results of a survey of patients about the level of satisfaction with the quality of patient education showed a significant increase compared to before the intervention (p < 0.001).
The ability to easily, completely, and quickly edit and record the provided education, eased the process of patient education and documentation.
A method to correct for the influence of bovine serum albumin-associated vitamin D metabolites in protein extracts from neonatal dried blood spots
Authors: Boelt Sanne Grundvad , Plana-Ripoll Oleguer , Albiñana Clara , Vilhjálmsson Bjarni , McGrath John J. , Cohen Arieh S. 
Published: June 2022

Abstract
Objective
We developed an assay to measure the concentration of 25 hydroxyvitamin D2 and D3 in protein extracts derived from stored neonatal dried blood spots. During this study, we postulated that these samples had been contaminated with exogenous vitamin D metabolites because of the addition of bovine serum albumin (BSA) as part of an extraction step undertaken 7 years earlier. The aim of the current study was to develop methods in order to adjust for this contamination.

Results
We identified between-plate variations in 25 hydroxyvitamin D2 and D3 concentrations which suggested the presence of three different BSA batches. Based on repeat extraction (without the addition of BSA) and testing of 395 samples, we developed models to correct for the exogenous 25 hydroxyvitamin D2 and D3. The regression models were Diff25OHD3 = − 8.2 + 1.8* Diff25OHD2 for low contamination, Diff25OHD3 = 23.8 + 1.7* Diff25OHD2 for middle contamination, and Diff25OHD3 = 14.3 + 3.0* Diff25OHD2 for high contamination. After these corrections, the three subsamples had comparable distributions within the expected range for both 25 hydroxyvitamin D2 and D3.
Horizontal transfer of a plasmid possessing mcr-1 marked with a single nucleotide mutation between Escherichia coli isolates from community residents
Authors: Yamamoto Yoshimasa , Higashi Ayano , Ikawa Kanoko , Hoang Hoa Thi Thanh , Yamaguchi Takahiro , Kawahara Ryuji , Noguchi Hideki , Nguyen Thang Nam , Khong Diep Thi , Tran Hoa Thi 
Published: June 2022

Abstract
Objectives
The widespread dissemination of phenotypic colistin-resistant (COR) bacteria in the community threatens public health. The horizontal gene transfer of the mobile colistin resistance gene via plasmids is thought to be one of the main mechanisms for dissemination. However, genotypic evidence to prove this in community settings is limited. This study used genome analysis to demonstrate the direct horizontal colistin resistance gene transfer via plasmids in isolates from the community.

Results
A total of 19 isolates of COR Escherichia coli from stool specimens of 23 residents from seven households in the Vietnamese community were assessed in this study. The whole-genome sequence data of isolates were acquired using a combination of DNBSEQ short-reads and Nanopore long-read sequencing. Analysis of genomic data was performed using online tools such as Geneious. Analysis of the genomic information of COR E. coli isolates revealed that the isolates from two residents of different households had a similar IncP1 plasmid possessing mcr-1.1, marked with a single nucleotide mutation at the same position. The study provided direct evidence to prove that mcr was horizontally transmitted among bacteria in community residents.
Preventing sexism and sexual harassment in medical schools by using Theater of the Oppressed as an interactive and reflexive tool
Authors: Lüthi Emmanuelle , Pichonnaz Lauriane , Schwarz Joëlle , Morier-Genoud Pascal , Dayer Caroline , Rrustemi Ilire , Schilter Léa , Berney Alexandre , John Caroline , Dubois Julie , Rodondi Pierre-Yves , Clair Carole 
Published: June 2022

Abstract
Objectives
Among the measures taken to combat sexism and sexual harassment, prevention courses for medical students are one possibility. We aimed to describe the process of implementing a training course on the prevention of sexism and sexual harassment for medical students in two Swiss medical schools by using the Theater of the Oppressed as an interactive and reflexive tool within the course. The purpose of this theater was to give the students the opportunity to express themselves and to collectively look for and discuss ways to combat and escape from oppressive situations.

Results
This collaborative, innovative, and interactive implementation showed that different forms of a training course can be implemented with similar objectives in an adaptable and transferable manner. The interactive and reflexive Theater of the Oppressed was an appropriate option to reach the objectives. Courses were based on identifying and acting on concrete problematic situations by focusing on individual, collective, and institutional resources. Students reported a high level of satisfaction.
dsSurvival: Privacy preserving survival models for federated individual patient meta-analysis in DataSHIELD
Authors: Banerjee Soumya , Sofack Ghislain N. , Papakonstantinou Thodoris , Avraam Demetris , Burton Paul , Zöller Daniela , Bishop Tom R. P. 
Published: June 2022

AbstractObjectiveAchieving sufficient statistical power in a survival analysis usually requires large amounts of data from different sites. Sensitivity of individual-level data, ethical and practical considerations regarding data sharing across institutions could be a potential challenge for achieving this added power. Hence we implemented a federated meta-analysis approach of survival models in DataSHIELD, where only anonymous aggregated data are shared across institutions, while simultaneously allowing for exploratory, interactive modelling. In this case, meta-analysis techniques to combine analysis results from each site are a solution, but an analytic workflow involving local analysis undertaken at individual studies hinders exploration. Thus, the aim is to provide a framework for performing meta-analysis of Cox regression models across institutions without manual analysis steps for the data providers.ResultsWe introduce a package (dsSurvival) which allows privacy preserving meta-analysis of survival models, including the calculation of hazard ratios. Our tool can be of great use in biomedical research where there is a need for building survival models and there are privacy concerns about sharing data.
Acute ischemic stroke: a rare complication of hump-nosed pit viper (Hypnale spp.) bite: a case report
Authors: Namal Rathnayaka R. M. M. K. , Nishanthi Ranathunga P. E. A. , Kularatne S. A. M. , Jayasinghe Sanath 
Published: June 2022

Abstract
Background
Hump-nosed pit viper is a medically important deadly venomous snake in Sri Lanka and is the commonest cause of venomous snakebites in the country. It frequently causes local effects and systemic manifestations such as acute kidney injury and coagulopathy that occur in less than 10% of all bites. This also includes some atypical presentations such as thrombotic microangiopathy and myocardial infarction. Currently, no antivenom is available for hump-nosed pit viper bites in Sri Lanka, and patients are managed with supportive treatment. This case illustrates an acute ischemic stroke following a hump-nosed viper bite, which is the second case in the literature.

Case presentation
A 71-year-old a Sinhalese male patient presented with left-sided hemiparesis with mouth deviation on day 2 of hump-nosed viper (Hypnale spp.) bite on the right foot. Non-contrast computed tomography of brain showed right ischemic stroke in internal capsule. He was given antiplatelets and statins and continued supportive treatment including limb physiotherapy and speech therapy. He recovered completely and was discharged on day 4 with clinic follow-up.

Conclusions
Physicians should be aware that ischemic cerebral infarcts may occur following hump-nosed pit viper bites.
A reduced level of the long non-coding RNA SNHG8 activates the NF-kappaB pathway by releasing functional HIF-1alpha in a hypoxic inflammatory microenvironment
Authors: Wang Chenxin , Yang Qiaolin , Han Yineng , Liu Hao , Wang Yue , Huang Yiping , Zheng Yunfei , Li Weiran 
Published: June 2022

Abstract
Background
A series of biochemical responses, including hypoxia and aseptic inflammation, occur in periodontal ligament cells (PDLCs) during periodontal tissue remodeling of orthodontic tooth movement (OTM). However, the role of long non-coding RNA (lncRNA) in these responses is still largely unknown. We investigated the role of the lncRNA SNHG8 in hypoxic and inflammatory responses during OTM and explored the underlying mechanisms.

Methods
The expression pattern of SNHG8, and hypoxic and inflammatory responses under compressive force were analyzed by qRT-PCR, immunohistochemistry, and western blotting, in vivo and in vitro. The effect of overexpression or knockdown of SNHG8 on the nuclear factor-kappaB (NF-κB) pathway was evaluated. RNA sequencing was performed for mechanistic analysis. The interaction between SNHG8 and hypoxia-inducible factor (HIF)-1α was studied using catRAPID, RNA immunoprecipitation, and RNA pulldown assays. The effect of the SNHG8–HIF-1α interaction on the NF-κB pathway was determined by western blotting.

Results
The NF-κB pathway was activated, and HIF-1α release was stabilized, in PDLCs under compressive force as well as in OTM model rats. The SNHG8 level markedly decreased both in vivo and in vitro. Overexpression of SNHG8 decreased the expression levels of inflammatory cytokines, the phosphorylation of p65, and the degradation of IκBα in PDLCs, whereas knockdown of SNHG8 reversed these effects. Mechanically, RNA sequencing showed that differentially expressed genes were enriched in cellular response to hypoxia after SNHG8 overexpression. SNHG8 binds to HIF-1α, thus preventing HIF-1 from activating downstream genes, including those related to the NF-κB pathway.

Conclusion
SNHG8 binds to HIF-1α. During OTM, the expression of SNHG8 dramatically decreased, releasing free functional HIF-1α and activating the downstream NF-κB pathway. These data suggest a novel lncRNA-regulated mechanism during periodontal tissue remodeling in OTM.
Modeling hypertrophic cardiomyopathy with human cardiomyocytes derived from induced pluripotent stem cells
Authors: Li Jiangtao , Feng Xin , Wei Xiang 
Published: June 2022

AbstractOne of the obstacles in studying the pathogenesis of hypertrophic cardiomyopathy (HCM) is the poor availability of myocardial tissue samples at the early stages of disease development. This has been addressed by the advent of induced pluripotent stem cells (iPSCs), which allow us to differentiate patient-derived iPSCs into cardiomyocytes (iPSC-CMs) in vitro. In this review, we summarize different approaches to establishing iPSC models and the application of genome editing techniques in iPSC. Because iPSC-CMs cultured at the present stage are immature in structure and function, researchers have attempted several methods to mature iPSC-CMs, such as prolonged culture duration, and mechanical and electrical stimulation. Currently, many researchers have established iPSC-CM models of HCM and employed diverse methods for performing measurements of cellular morphology, contractility, electrophysiological property, calcium handling, mitochondrial function, and metabolism. Here, we review published results in humans to date within the growing field of iPSC-CM models of HCM. Although there is no unified consensus, preliminary results suggest that this approach to modeling disease would provide important insights into our understanding of HCM pathogenesis and facilitate drug development and safety testing.
Adipose-derived stromal/stem cells are verified to be potential seed candidates for bio-root regeneration in three-dimensional culture
Authors: Yuan Yu , Zhang Xiaonan , Zhan Yuzhen , Tang Song , Deng Pingmeng , Wang Zhenxiang , Li Jie 
Published: June 2022

Abstract
Background
Bio-root regeneration is a promising treatment for tooth loss. It has been reported that dental-derived stem cells are effective seed cells for bio-root construction, but further applications are limited by their few sources. Human adipose tissues have a wide range of sources and numerous studies have confirmed the ability of adipose-derived stromal/stem cells (ASCs) in regenerative medicine. In the current study, the odontogenic capacities of ASCs were compared with dental-derived stem cells including dental follicle cells (DFCs), and stem cells from human exfoliated deciduous teeth (SHEDs).

Methods
The biological characteristics of ASCs, DFCs, and SHEDs were explored in vitro. Two-dimensional (2D) and three-dimensional (3D) cultures were compared in vitro. Odontogenic characteristics of porcine-treated dentin matrix (pTDM) induced cells under a 3D microenvironment in vitro were compared. The complexes (cell/pTDM) were transplanted subcutaneously into nude mice to verify regenerative potential. RNA sequencing (RNA-seq) was used to explore molecular mechanisms of different seed cells in bio-root regeneration.

Results
3D culture was more efficient in constructing bio-root complexes. ASCs exhibited good biological characteristics similar to dental-derived stem cells in vitro. Besides, pTDM induced ASCs presented odontogenic ability similar to dental-derived stem cells. Furthermore, 3D cultured ASCs/pTDM complex promoted regeneration of dentin-like, pulp-like, and periodontal fiber-like tissues in vivo. Analysis indicated that PI3K-Akt, VEGF signaling pathways may play key roles in the process of inducing ASCs odontogenic differentiation by pTDM.

Conclusions
ASCs are potential seed cells for pTDM-induced bio-root regeneration, providing a basis for further research and application.

Graphical Abstract
Phosphatized adductor muscle remains in a Cenomanian limid bivalve from Villers-sur-Mer (France)
Authors: Klug Christian , Hüne Liane , Roth Rosemarie , Hautmann Michael 
Published: June 2022

AbstractSoft-tissue preservation in molluscs is generally rare, particularly in bivalves and gastropods. Here, we report a three-dimensionally preserved specimen of the limid Acesta clypeiformis from the Cenomanian of France that shows preservation of organic structures of the adductor muscles. Examination under UV-light revealed likely phosphatisation of organic remains, which was corroborated by EDX-analyses. We suggest that the parts of the adductor muscles that are very close to the attachment are particularly resistant to decay and thus may be preserved even under taphonomic conditions usually not favouring soft-tissue fossilisation.
Metabolic phenotyping of hand automatisms in mesial temporal lobe epilepsy
Authors: Mo Jiajie , Wang Yao , Zhang Jianguo , Cai Lixin , Liu Qingzhu , Hu Wenhan , Sang Lin , Zhang Chao , Wang Xiu , Shao Xiaoqiu , Zhang Kai 
Published: June 2022

AbstractPurposeHand automatisms (HA) are common clinical manifestations in mesial temporal lobe epilepsy. However, the location of the symptomatogenic zone (EZ) in HA as well as the networks involved, are still unclear. To have a better understanding of HA underlying mechanisms, we analyzed images from interictal [18F] fluorodeoxyglucose-positron emission tomography (FDG-PET) in patients with mesial temporal lobe epilepsy (mTLE).MethodsWe retrospectively recruited 79 mTLE patients and 18 healthy people that substituted the control group for the analysis. All patients underwent anterior temporal lobectomy and were seizure-free. Based on the semiology of the HA occurrence, the patients were divided into three subgroups: patients with unilateral HA (Uni-HA), with bilateral HA (Bil-HA) and without HA (None-HA). We performed the intergroup comparison analysis of the interictal FDG-PET images and compared the functional connectivity within metabolic communities.ResultsOur analysis showed that the metabolic patterns varied among the different groups. The Uni-HA subgroup had significant differences in the extratemporal lobe brain areas, mostly in the ipsilateral supplementary motor area (SMA) and middle cingulate cortex (MCC) when compared to the healthy control group. The Bil-HA subgroup demonstrated that the bilateral SMA and MCC areas were differentially affected, whereas in the None-HA subgroup the differences were evident in limited brain areas. The metabolic network involving HA showed a constrained network embedding the SMA and MCC brain regions. Furthermore, the increased metabolic synchronization between SMA and MCC was significantly correlated with HA.ConclusionThe metabolic pattern of HA was most conspicuous in SMA and MCC brain regions. Increased metabolic synchronization within SMA and MCC was considered as the major EZ of HA.
FANCC deficiency mediates microglial pyroptosis and secondary neuronal apoptosis in spinal cord contusion
Authors: Xia Mingjie , Li Xinyu , Ye Suhui , Zhang Qinyang , Zhao Tianyu , Li Rulin , Zhang Yanan , Xian Minghan , Li Tianqi , Li Haijun , Hong Xin , Zheng Shengnai , Qian Zhanyang , Yang Lei 
Published: June 2022

AbstractBackgroundTraumatic spinal cord injury (SCI)-induced neuroinflammation results in secondary neurological destruction and functional disorder. Previous findings showed that microglial pyroptosis plays a crucial role in neuroinflammation. Thus, it is necessary to conduct a comprehensive investigation of the mechanisms associated with post-SCI microglial pyroptosis. The Fanconi Anemia Group C complementation group gene (FANCC) has been previously reported to have an anti-inflammation effect; however, whether it can regulate microglial pyroptosis remains unknown. Therefore, we probed the mechanism associated with FANCC-mediated microglial pyroptosis and neuroinflammation in vitro and in vivo in SCI mice.MethodsMicroglial pyroptosis was assessed by western blotting (WB) and immunofluorescence (IF), whereas microglial-induced neuroinflammation was evaluated by WB, Enzyme-linked immunosorbent assays and IF. Besides, flow cytometry, TdT-mediated dUTP Nick-End Labeling staining and WB were employed to examine the level of neuronal apoptosis. Morphological changes in neurons were assessed by hematoxylin–eosin and Luxol Fast Blue staining. Finally, locomotor function rehabilitation was analyzed using the Basso Mouse Scale and Louisville Swim Scale.ResultsOverexpression of FANCC suppressed microglial pyroptosis via inhibiting p38/NLRP3 expression, which in turn reduced neuronal apoptosis. By contrast, knockdown of FANCC increased the degree of neuronal apoptosis by aggravating microglial pyroptosis. Besides, increased glial scar formation, severe myelin sheath destruction and poor axon outgrowth were observed in the mice transfected with short hairpin RNA of FANCC post SCI, which caused reduced locomotor function recovery.ConclusionsTaken together, a previously unknown role of FANCC was identified in SCI, where its deficiency led to microglia pyroptosis, neuronal apoptosis and neurological damage. Mechanistically, FANCC mediated microglia pyroptosis and the inflammatory response via regulating the p38/NLRP3 pathway.
A coherent accumulation detection method based on SA-DPT for highly manoeuvring targets
Authors: Pang Cunsuo , Hou Huiling , Guo Hualing 
Published: June 2022

AbstractThe emergence of highly manoeuvrable weak targets has led to serious degradation or even failure of traditional radar detection. In this paper, a coherent accumulation algorithm based on a combination of a scaling algorithm (SA) and a discrete polynomial-phase transform (DPT) is proposed, and the calculation burden and detection performance were evaluated. The algorithm first performs fewer speed parameter compensations based on an SA for the transmitted signal and selects the effective delay range of the target signal. Second, for the extracted echo signal, the DPT algorithm is used to estimate the target speed and acceleration. The proposed algorithm analyses the influence of the time delay range, the compensation speed and the delay unit on the detection performance and provides the improvement degree of the output SNR and the amount of complex multiplication. Finally, the experimental data verify the effectiveness of the proposed algorithm in terms of accumulation gain and parameter estimation. This method is for suboptimal estimation and requires much less computation than joint search methods, but it performs better than cross-correlation methods.
An improved secure designated server public key searchable encryption scheme with multi-ciphertext indistinguishability
Authors: Guo Junling , Han Lidong , Yang Guang , Liu Xuejiao , Tian Chengliang 
Published: June 2022

AbstractIn the cloud, users prefer to store their sensitive data in encrypted form. Searching keywords over encrypted data without loss of data confidentiality is an important issue. In 2004, Boneh et al. proposed the first public-key searchable encryption scheme which allows users to search by the private key. However, most existing public-key searchable encryption schemes are vulnerable to keyword guessing attack and can not satisfy multi-ciphertext indistinguishability. In this paper, we construct a secure designated server public-key searchable encryption based on Diffie-Hellman problem. Our security analysis shows that our proposed scheme can resist against keyword guessing attack and provide multi-ciphertext indistinguishability for any adversity. Furthermore, the proposed scheme can achieve multi-trapdoor privacy for external attackers. Moreover, the simulation results between our scheme and previous schemes demonstrate our new scheme is suitable for practical application.
Attribute reduction based scheduling algorithm with enhanced hybrid genetic algorithm and particle swarm optimization for optimal device selection
Authors: Nwogbaga Nweso Emmanuel , Latip Rohaya , Affendey Lilly Suriani , Rahiman Amir Rizaan Abdul 
Published: June 2022

AbstractThe applications of the Internet of Things in different areas and the resources that demand these applications are on the increase. However, the limitations of the IoT devices such as processing capability, storage, and energy are challenging. Computational offloading is introduced to ameliorate the limitations of mobile devices. Offloading heavy data size to a remote node introduces the problem of additional delay due to transmission. Therefore, in this paper, we proposed Dynamic tasks scheduling algorithm based on attribute reduction with an enhanced hybrid Genetic Algorithm and Particle Swarm Optimization for optimal device selection. The proposed method uses a rank accuracy estimation model to decide the rank-1 value to be applied for the decomposition. Then canonical Polyadic decomposition-based attribute reduction is applied to the offload-able task to reduce the data size. Enhance hybrid genetic algorithm and particle Swarm optimization are developed to select the optimal device in either fog or cloud. The proposed algorithm improved the response time, delay, number of offloaded tasks, throughput, and energy consumption of the IoT requests. The simulation is implemented with iFogSim and java programming language. The proposed method can be applied in smart cities, monitoring, health delivery, augmented reality, and gaming among others.