bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Efficacy of Metarhizium anisopliae (Metchnikoff) Sorokin against Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) under in vitro conditions”
Authors: Raypuriya Neelesh , SB Das , AK Bhowmick , V Pandey 
Published: August 2022

Abstract
BackgroundThe Helicoverpa armigera, Hubner (Lepidoptera: Noctuidae), is the most important economic pest. In the larval stage, the pest feeds on tomato fruits and seriously damages the fruits. Therefore, to reduce the overuse of chemical pesticides in tomato crops, microbial control is a key ingredient for sustainable crop production. Most toxic substances produced by microbial pathogens have been identified as peptides, but they are very different in terms of structure, toxicity and specificity. Therefore, the objective of the present investigation was to determine the bioefficacy of the entomopathogenic fungus, 12 Metarhizium anisopliae (Metchnikoff) Sorokin, isolates against 2nd instar larvae of the tomato fruit borer Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) under in vitro conditions. ResultsIn vitro studies an bioefficacy of 12 Metarhizium anisopliae (Metschnikoff) (Sorokin) isolates (M1, M2, M3, M4, M5, M6, M7, M8, M9, M10, M11 and M12) at four different concentrations of 1x1010, 1x109, 1x108, 1x107 against 2nd instar Helicoverpa armigera (Hubner) larvae revealed that all the strains were pathogenic to the target pest at all spore concentrations. However, among them isolates M3 and M7, were most effective and recorded 95 and 85% larval mortality with LT50 value of 47.82 and 56.75 hours at the lowest spore concentration (1x1010 spores ml-1) and LC50 values of 5.06x105 and 2.29x107 spore ml−1, respectively. The lowest mortality (62.50%) was recorded in isolate M4 at 5 days after inoculation. ConclusionThese findings suggested that the adverse effect of most virulent M. anisopliae isolates M3 and M7 were compatible and most virulent to the target pest and that the simultaneous use may be helpful for managing H. armigera, 2nd instar larvae.
Maternal transmission bias of inherited genetic variants in severe early-onset obesity
Authors: de Pieri Francesc Bou , Serra-Juhé Clara , Martín-Rivada Álvaro , Martos-Moreno Gabriel , Argente Jesus , Perez-Jurado Luis 
Published: September 2022

Abstract
Background:
Obesity is highly influenced by genetics, with stronger genetic component in severe early-onset obesity (EOO). Heterozygous rare sequence variants (RSVs) affecting genes of the leptin-melanocortin pathway and hypothalamic function cause EOO, with variable expressivity and incomplete penetrance. We aimed to define whether and how parental inheritance could influence expressivity and penetrance.
Methods:
Observational study, systematic review and meta-analysis of reported inherited likely pathogenic heterozygous RSVs associated to non-syndromic EOO in 17 candidate genes was performed. Parental phenotype and proband’s birthweight were obtained when available. Child-feeding practices in transmitting and non-transmitting mothers were compared in a subsample using a validated questionnaire.
Results:
We identified 38 studies reporting 216 children with EEO and inherited heterozygous RSVs in candidate genes. A significant maternal over-transmission was observed (61.6%, 1.66:1, p = 0.0004), higher for RSVs inherited from severely obese parents (84%, 5.2:1, p = 0.0005). Patients with maternally inherited RSVs had an increased birthweight compared with patients with non-maternally inherited RSVs. Transmitting mothers manifest lower perceived responsibility for child feeding behavior when compared to non-transmitting mothers of either obese or non-obese children.
Conclusions:
A maternal over-transmission bias of inherited dominant RSVs disrupting genes of the leptin-melanocortin pathway contributes to severe EOO. Thus, synergistic effects of RSVs present in both, transmitting mothers and their children, can explain the more severe phenotype in the children. Prenatal factors during gestation and postnatal deregulation of eating behavior due to maternal carelessness may contribute to this bias. Identification of this risk factor may be useful for early intervention to prevent long term consequences of severe EOO.
The mitochondrial genomes of Crispatotrochus rubescens and Crispatotrochus rugosus (Hexacorallia; Scleractinia): new insights on the phylogeny of the family Caryophylliidae.
Authors: Vaga Claudia Francesca , Seiblitz Isabela Galvao de Lossio , Capel Katia Cristina Cruz , Kitahara Marcelo Visentini 
Published: August 2022

Abstract

BackgroundCaryophylliidae is one of the most diverse scleractinian families, however it was recovered as polyphyletic in multiple molecular studies. Recently, the mitochondrial gene order was proposed as a character for a taxonomic revision of the family. Here we describe the first mitogenome of the caryophylliid genusCrispatotrochus, whose phylogenetic position remains uncertain.Methods and resultsThe complete mitochondrial genomes ofCrispatotrochus rubescensandCrispatotrochus rugosuswere sequenced, assembled, and annotated. The two mitogenomes are identical and circular, have a length of 16,536 bp, a GC content of 35.9%, and contain 13 protein-coding genes, 2 ribosomal RNAs and 2 transfer RNAs. Both species have a transposition of a three-genes block –cob,nad2, andnad6– similarly to a group of caryophylliid genera that were recovered as monophyletic and include the type species (Caryophyllia) of the family. The phylogenetic analyses recoveredCrispatotrochuswithin the clade that presents the gene rearrangement and specifically as sister taxa of the genusCaryophyllia, a result consistent with previous studies and the similar gross morphology of the two genera.ConclusionsWe determined the mitochondrial genomes of the genusCrispatotrochusto investigate their relations within Scleractinia. Results from this study provide insights on the phylogenetic position of the genus and corroborate that the mitochondrial gene order could be used as taxonomic character for the family Caryophylliidae.
Geometry-Aware View ReconstructionNetwork for Light Field ImageCompression
Authors: Zhang Youzhi , Wan Lifei , Mao Yifan , Huang Xinpeng , Liu Deyang 
Published: September 2022

Abstract
Light Field (LF) imaging empowers many attractive applications by simultaneously recording spatial and angular information of light rays. In order to meet the challenges of LF storage and transmission, many view reconstruction-based LF compression methods are put forward. However, occlusion issue and under-exploitation of LF rich structure information limit the view reconstruction qualities, which further influence LF compression efficiency. In order to alleviate these problems, in this paper, we propose a geometry-aware view reconstruction network for LF compression. In our method, only sparsely-sampled LF views are encoded, which are further used as priors to reconstruct the un-sampled LF views at the decoder side. The proposed reconstruction process contains two stages including geometry-aware reconstruction and texture refinement. The geometry-aware reconstruction stage utilizes a multi-stream framework, which can fully explore LF spatial-angular, location and geometry information. The texture refinement stage can adequately fuse such rich LF information to further improve LF reconstruction quality. Comprehensive experimental results validate the superiority of the proposed method. The rate-distortion performance and the perceptual quality of reconstructed views further demonstrate that the proposed method can save more bitrate while increasing LF reconstruction quality.
Experimental study on compression-shear fracture evolution process of locked segment of rock-like based on multivariate parameters
Authors: Jiang Tong , Wan Li , Huang Kun , He Tianle , Liu Chen , Zhang ChaoLi , Li Longfei 
Published: September 2022

Abstract
Locked segment of rock-like material specimens with different brittleness index were prepared by combining materials in different ratios. Compression-shear tests of rock-like material specimens with different brittleness degree were carried out by acoustic emission and Laser Doppler Vibrometer measurement. The fracture evolution process of the specimen was divided into three stages through the displacement-time curve: compaction stage and elastic deformation stage, constant deformation stage and accelerated deformation stage. The natural frequency responded obviously at the boundary point of the constant deformation stage and accelerated deformation stage, the point of natural frequency steeply dropped corresponds to the starting point of constant deformation stage, which is defined as the damage point. The point of substantial increase in the natural frequency corresponds to the starting point of accelerated deformation stage, which is defined as expansion point. There were obvious critical instability information before the failure of specimens: The natural frequency showed rise, steep drop and stable jitter; The ringing count rate and energy rate increased near the critical instability point; The b-value continued to decrease at a low level before the peak strength. Tensile failure was the main failure mode of the three specimens with different brittleness degree, the tensile failure degree increased with the increase of brittleness degree. The energy released by the specimen at failure moment increased with the increase of brittleness degree, and the b-value decreased with the increase of brittleness degree. The degree of brittleness is one of the important indexes to study the locked segment.
SARS-CoV-2 S protein triggers lung and intestinal epithelial cell damage via TGF-β/Smad2/3-mediated inflammatory cytokine production
Authors: huang Chao , hua-shi huang , weixuan huang , haosheng liu , shen weizeng 
Published: September 2022

Abstract
Background and objective Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continues to ravage the world. Despite many prevention and control measures, morbidity and mortality have not decreased due to SARS-CoV-2-induced organ damage, which occur via unknown mechanisms. Methods Primary Human small intestinal mucosa epithelial cells (HSIMECs), human colonic epithelial cells (HCoEpiCs), and human type II alveolar epithelial cells (hTIIAECs) were treated with recombinant SARS-CoV-2 S protein for 48 h. Cell morphology, permeability, and viability were detected. The expression of cysteinyl aspartate specific proteinase 3 (caspase 3) and B-cell lymphoma-2 (Bcl-2) was examined using Western blotting. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the levels of inflammatory cytokines in the supernatant. Apoptosis was observed using a Hoechst 33258 Staining Kit. SB431542 and BAY11-7082 were used to inhibit transforming growth factor-β (TGF-β)/suppressor of mothers against decapentaplegics (Smads) and the inhibitor of kappa B kinase (IKK)/nuclear factor-κB (NF-κB) pathways, respectively. Results S protein produced no obvious changes in morphology, but decreased cell viability and increased permeability were observed in a concentration- and time-dependent manner compared to the control (P<0.05). Apoptosis occurred with increased caspase 3 and decreased Bcl-2 (P<0.05). S protein stimulated a disordered secretion of cytokines, including interleukin (IL)-6, IL-10, tumour necrosis factor α (TNF-α), and IL-13 (P<0.05). Suppression of TGF-β/Smad3, but not the IKK/NF-κB, pathway relieved the damage to colon cells caused by the S protein in HSIMECs and HCoEpiCs and inhibited apoptosis mediated by TNF-α and granulocyte-macrophage colony-stimulating factor (GM-CSF) in hTIIAECs. Conclusion The SARS-CoV-2 S protein damaged intestine and lung cells, which was associated with cytokine production and the induction of apoptosis mediated by the TGF-β/Smad3, but not the NF-κB, pathway.
MetaGenePipe: An Automated, Portable Pipeline for Contig-based Functional and Taxonomic Analysis
Authors: Shaban Babak , Quiroga Maria del Mar , Turnbull Robert , Tescari Edoardo , Cao Kim-Anh Lê , Verbruggen Heroen 
Published: September 2022

Abstract

MetaGenePipe (MGP) is an efficient, flexible, portable, and scalable metagenomics pipeline that uses performant bioinformatics software suites and genomic databases to create an accurate taxonomic and functional characterization of the prokaryotic fraction of sequenced microbiomes. Written in the Workflow Definition Language (WDL), MGP produces output that is useful in its default form, or that can be used for further downstream analysis. MGP is a pipeline-development best practice tool which uses Singularity for containerization and includes a setup script that downloads the necessary databases for setup. The source code for MGP is freely available and is distributed under the Apache 2.0 license.
Computational Investigation of Functional Water Molecules Upon GPCR Bound to G protein or Arrestin
Authors: Hu Jiaqi , Sun Xianqiang , Kang Zhengzhong , Cheng Jianxin 
Published: August 2022

Abstract
G protein-coupled receptors (GPCRs) are membrane proteins, which constitutes the largest family of drug targets. The activated GPCR can bound either G protein or Arrestin to accomplish its activation. Water molecules were reported to play an intriguing role in GPCR activation. Nevertheless, reported studies are focused in the hydrophobic helical bundle region. How water molecules function upon GPCR bound either G protein or Arrestin is rarely studied. To address this issue, we carried out computational studies on water molecules in both GPCR/G protein complexes and GPCR/Arrestin complexes. Using the inhomogeneous fluid theory (IFT), hydration sites of GPCRs in G protein or Arrestin binding state were located and their functions were comprehensively analyzed. In the interaction surface of GPCR-G protein/Arrestin, a lot of water molecules were found. In addition, we found that the number of water molecules on the interaction surface of GPCR-G protein/Arrestin system is highly associated with the insertion depth of the α5-helix and “Finger Loop”. We observed that water molecules near the interaction surface of GPCR-G protein/Arrestin exhibit great differences. Most G protein-related structures attract more function water molecules than Arrestin-associated structures. The G protein-related GPCRs show more potent binding water molecules and water-mediated hydrogen-bond compared to Arrestin complexes. Moreover, a small amount of water molecules is observed in the NPxxY region, while a large number of water molecules are in the orthosteric pocket and form rich interaction networks. Our results provide a comprehensive and deep understanding on the hydration sites in GPCRs and may have important implications for GPCR-targeted drug design with functional selectivity.
WITHDRAWN: The role of cannabis clinics in the health system: A qualitative study of physicians’ views in New Zealand
Authors: Withanarachchie Vinuli , Rychert Marta , Wilkins Chris 
Published: August 2022

Abstract
Background: Privately-owned cannabis clinics have sprung up in many jurisdictions where medicinal cannabis has been legalised and provide an alternative pathway for patients who are unable or unwilling to access a prescription for cannabis-based medicinal products from their usual healthcare providers. Aims: This study aimed to explore physicians’ views on cannabis clinics, including their perceptions of the role of cannabis clinics play in the wider health system.Methods: A qualitative study using in-depth, semi-structured interviews with thirty-one physicians affiliated with private and community clinics in New Zealand (including cannabis clinicians, GPs, and specialist doctors). The interviews were conducted from July to December 2021. Data were transcribed and analysed using inductive thematic analysis. Results: Cannabis clinicians positioned themselves as (1) “service providers”, facilitating consumer access to cannabis prescriptions and products, and (2) “educators”, providing advice to patients and the wider physician community. While general practitioners and specialists (non-cannabis clinics) recognised the benefits of specialised cannabis clinics (i.e., knowledge of products and a non-judgmental environment), they questioned the limited evidence of clinical efficacy for cannabis, potential financial conflicts of interests of cannabis clinicians that may blur their clinical judgement, and the risk of compartmentalising patients’ healthcare.Conclusions: Our paper raises a number of challenges with attempting to integrate cannabis clinics into the wider health system.
Establishment of a CaCC-based cell model and method for high-throughput screening of M3 receptor drugs
Authors: Liu Xueying , Ju Xiaohong , Wu Mingda , Wang Ximin , Hong Qiyuan , Xing Wenzhu , Xu Meng , Hu Cheng , Hao Feng 
Published: August 2022

Abstract
Muscarinic acetylcholine receptor subtype 3 (M3 receptor) is a G Protein-Coupled Receptor (GPCR) that mediates many important physiological functions. Currently, no M3 receptor drugs with high specificity, high activity, and few side effects have been developed, and there is a lack of methods suitable for high-throughput screening of drugs with GPCRs. In this study, we established an efficient and sensitive drug cell screening model and method for targeting M3 receptors based on calcium-activated chloride channels (CaCCs). This screening model consists of Fischer rat thyroid follicular epithelial (FRT) cells that endogenously express M3 receptors, CaCCs, and the indicator YFP-H148Q/I152L. We verified that the model can sensitively detect changes in intracellular Ca2+ concentration using fluorescence quenching kinetics experiments, confirmed the screening function of the model by applying available M3 receptor drugs, and also evaluated the good performance of the model in high-throughput screening.
Missing Data Recovery in the e-health context based on Machine Learning models
Authors: Mahfoudhi Sami , Rahmany Ines , Freihat Mushira , Moulahi Tarek 
Published: August 2022

Abstract
Diabetes mellitus is a set of metabolic illnesses characterized by abnormally high blood sugar levels. In 2017, 8.8% of the world’s population had diabetes. By 2045, it is expected that this percentage will have risen to approximately 10%. Missing data, a prevalent problem even in a well-designed and controlled study, can have a major impact on the conclusions that can be derived from the available data. Missing data may decrease a study’s statistical validity and lead to erroneous results due to distorted estimations. In this study, we hypothesize that (a) replacing missing values using machine learning techniques rather than the mean value and group mean value and (b) using SVM kernel RBF classifier will result in the highest level of accuracy in comparison to traditional techniques such as DT, RF, NB, SVM, AdaBoost, and ANN. The classification results improved significantly when using regression to replace the missing values over the group median or the mean. This is a 10% improvement over previously developed strategies that have been reported in the literature.
Groundwater quality assessment for drinking, irrigation, and human health hazards with special emphasis on Nitrate pollution in Uddanam area, Srikakulam district of Andhra Pradesh, India
Authors: y vinod kumar , v gope naik , e balaji , g veeraswamy 
Published: September 2022

Abstract

This study's goal is to evaluate the Uddanam region's groundwater quality for drinking, agricultural use, and potential health risks to people, with a focus on nitrates. From the research region, 50 groundwater samples were taken in both pre- and post-monsoons and analyzed for pH, EC, TDS, TH, TA, Ca2+, Mg2+, Na+, K+, CO3, HCO3−, Cl−, SO42−, NO3−, F−, and Si, among other physic-chemical parameters, TDS, total hardness, nitrates and silica are the highest ions among all chemical parameters. May's findings indicate that kidney illness is a result of higher above said parameters. In addition, the chemical data intensity is represented using spatial interpolation techniques in Arc GIS 10.1 software, and intensity maps of the research region are prepared. The irrigation metrics salt absorption ratio (SAR), Kelley's ratio (KR), and percent sodium (%Na) are all within the research area's allowed limits.In the study area, pre and post-monsoon nitrate total hazard index (children, men, and women) affected percentages are 94%, 53%, 37% and 94%, 51%, 29% respectively, whereas the dermal there is no significant effect on the human body. The Gibbs diagram shows that the disintegration of rock formations is responsible for the groundwater chemistry and is further supported by scatter plots. Chemical elements are generated in the study due to geology such as Khondalite and alluvial soil disintegration. The excess silica percentage may lead to affect kidney-born disease in the study area and hence adequate water treatment is required prior to use.
Analysis of the Meningiomas` hormonal profile in patients with Meningioma and Breast Cancer
Authors: Finger Guilherme , Prevedello Daniel M , Godoy Bruno Loyola , Carvalho Rodolfo Figueiredo , Pinto Luciana Wernersbach , Valverde Priscila , Souto Antonio Aversa do 
Published: August 2022

Abstract
Purpose
The objective of this study is to evaluate the meningioma`s hormone receptor expression in patients who presented synchronous or metachronous meningioma and breast cancer and compare its profile to the non-breast cancer associated meningiomas described in the literature.
Methods
The authors conducted a retrospective descriptive study evaluating all cases of synchronous and metachronous meningiomas and breast cancer from January 1st, 1990 to December 31st, 2020. The study was approved by the Ethics Review Board. Variables related to meningioma (age and year of diagnosis, histological grade and subtype, topography, the presence of progesterone and/or estrogen receptors) and to breast cancer (age and year of diagnosis, the histological grade and subtype, the presence of progesterone and/or estrogen receptors) were collected on the hospital`s electronic health records.
Results
A total of 12 cases were included in the study, being 2 (17%) synchronous and 10 (83%) metachronous. The mean age at meningioma diagnosis was 60.58 (± 10,99). The most frequent intracranial topography was convexity and sphenoid wing meningiomas (25% each). Ten patients (83%) had grade 1 meningiomas and 2 patients (16.6%) presented with grade 2 meningiomas. Eleven cases of meningiomas expressed progesterone receptors (91.67%) while 3 cases expressed estrogen receptors (25%). One patient did not express hormonal receptor.
Conclusion
The WHO histological analysis and steroid receptors profile did not seem to be different in the meningiomas included in this sample when compared to the description of no-breast cancer associated meningiomas.
Body stalk anomaly complicated by complex congenital heart disease: first-trimester ultrasonic diagnosis and fetal autopsy
Authors: Chen Shiyu , Dai Xiaohui , Liu Hanmin , Chen Jiao 
Published: August 2022

Abstract
Body stalk anomaly (BSA) is a relatively rare and severe congenital malformation. Most reported heart anomalies occurring in combination with BSA are ectopic hearts. We herein report a rare case of BSA complicated by complex congenital heart disease prenatally diagnosed in the first trimester by ultrasound and confirmed by autopsy. This case emphasizes that in addition to the typical anomalies, cardiac structural abnormalities are also worthy of attention in cases of BSA.