bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inversion of the satellite observations of the tidally induced magnetic field in terms of 3-D upper-mantle electrical conductivity: method and synthetic tests
Authors: Šachl L , Velímský J , Fullea J , Martinec Z 
Published: January 2022

SUMMARY
The interaction of the oceanic tidal flow with the Earth’s main magnetic field provides a powerful natural source of electromagnetic (EM) energy suitable for suboceanic upper-mantle electrical conductivity sounding. In this paper, we have developed and tested a new frequency-domain, spherical harmonic-finite element approach to the inverse problem of global EM induction. It is set up for an effective inversion of satellite-observed tidally induced magnetic field in terms of 3-D structure of the electrical conductivity in the suboceanic upper mantle. Before proceeding to the inversion of Swarm-derived models of tidal magnetic signatures, we have performed a series of parametric studies, using the 3-D conductivity model WINTERC-e as a testbed. The WINTERC-e model has been derived using state-of-the-art laboratory conductivity measurements of mantle minerals, and thermal and compositional model of the lithosphere and upper mantle WINTERC-G. The latter model is based on the inversion of global surface waveforms, satellite gravity and gradiometry measurements, surface elevation and heat flow data in a thermodynamically self-consistent framework. Therefore, the WINTERC-e model, independent of any EM data, represents an ideal target for synthetic tests of the 3-D EM inversion. We tested the impact of the truncation degree of the spherical-harmonic expansion of the M2 tidal signal, the effect of random noise in synthetic data and inclusion of the N2 and O1 tidal constituents on the ability to recover the suboceanic upper-mantle conductivity structure. We demonstrate that with suitable regularization we can successfully reconstruct the 3-D upper-mantle conductivity beneath world oceans. In the ideal noise-free case, the correlation coefficient between the target and recovered conductivity is greater than 0.8 in the 150–270 km depth range.
French validation of the e-PROSCeSS questionnaire: stakeholder perceptions of the health promoting sports club
Authors: Johnson Stacey , Vuillemin Anne , Epstein Jonathan , Geidne Susanna , Donaldson Alex , Tezier Benjamin , Kokko Sami , Van Hoye Aurélie 
Published: January 2022

Summary
The health promoting sports club describes the development of the settings-based approach in sports clubs. Based on this model, a questionnaire was developed to measure health promotion perceptions in sports clubs (e-PROSCeSS). The objective of this study was to assess the psychometric properties of a French version of the e-PROSCeSS measurement tool. The questionnaire includes three scales measuring stakeholder’s perceptions of club (macro), managers (meso) and coaches (micro) activities toward promoting health. Five steps were undertaken to assess perceptions. First, scales were translated into French. Second, each item’s content clarity was tested in three populations: managers, coaches, sports participants. Third, descriptive statistics were analyzed for each scale. Fourth, confirmatory factorial analysis was used to select items for each level. Predictive validity found positive relationships between health promotion perceptions and managing/coaching positions, and negative relationships with drop-out intentions. Positive relationships were found between sports participants’ perceptions of health promotion and their self-rated performance and quality of life, while negative relationships were detected with drop-out intentions. The French e-PROSCeSS subscales showed good psychometric properties in measuring health determinants at multiple levels. However, the tool did not measure health promotion as an overarching concept that questions its applicability in the sports club setting.
E3 ligase Smurf1 protects against misfolded SOD1 in neuronal cells by promoting its K63 ubiquitylation and aggresome formation
Authors: Dong Lei , Liu Liqun , Li Yang , Li Wenxuan , Zhou Liying , Xia Qin 
Published: January 2022

AbstractK63-linked polyubiquitination of the neurodegenerative disease-associated misfolded protein copper-zinc superoxide dismutase 1 (SOD1) is associated with the formation of inclusion bodies. Highly expressed E3 ligase Smad ubiquitylation regulatory factor 1 (Smurf1) promotes cellular homeostasis through the enhanced capability of aggregate degradation. However, it is not well explored the role of Smurf1 in the dynamics of SOD1 aggresomes. In this study, we report that Smurf1 promotes the recruitment of SOD1 to form aggresomes. Mechanistically, Smurf1 interacts with mutant SOD1 to promote aggresome formation by modification of its K63-linked polyubiquitination. Moreover, overexpressed Smurf1 enhances mutant SOD1 aggresome formation and autophagic degradation to prevent cell death. Thus, our data suggest that Smurf1 plays an important role in attenuating protein misfolding-induced cell toxicity by both driving the sequestration of misfolded SOD1 into aggresomes and autophagic degradation.
Multiomics analyses reveal early metabolic imbalance and mitochondrial stress in neonatal photoreceptors leading to cell death in Pde6brd1/rd1 mouse model of retinal degeneration
Authors: Jiang Ke , Mondal Anupam Kumar , Adlakha Yogita K , Gumerson Jessica , Aponte Angel , Gieser Linn , Kim Jung-Woong , Boleda Alexis , Brooks Matthew J , Nellissery Jacob , Fox Donald A , Balaban Robert , Covian Raul , Swaroop Anand 
Published: January 2022

Abstract
Retinal diseases exhibit extensive genetic heterogeneity and complex etiology with varying onset and severity. Mutations in over 200 genes can lead to photoreceptor dysfunction and/or cell death in retinal neurodegeneration. To deduce molecular pathways that initiate and/or drive cell death, we adopted a temporal multiomics approach and examined molecular and cellular events in newborn and developing photoreceptors before the onset of degeneration in a widely-used Pde6brd1/rd1 (rd1) mouse, a model of autosomal recessive retinitis pigmentosa caused by PDE6B mutations. Transcriptome profiling of neonatal and developing rods from the rd1 retina revealed early downregulation of genes associated with anabolic pathways and energy metabolism. Quantitative proteomics of rd1 retina showed early changes in calcium signaling and oxidative phosphorylation, with specific partial bypass of complex I electron transfer, which precede the onset of cell death. Concurrently, we detected alterations in central carbon metabolism, including dysregulation of components associated with glycolysis, pentose phosphate and purine biosynthesis. Ex vivo assays of oxygen consumption and transmission electron microscopy validated early and progressive mitochondrial stress and abnormalities in mitochondrial structure and function of rd1 rods. These data uncover mitochondrial overactivation and related metabolic alterations as determinants of early pathology and implicate aberrant calcium signaling as an initiator of higher mitochondrial stress. Our studies thus provide a mechanistic framework with mitochondrial damage and metabolic disruptions as early drivers of photoreceptor cell death in retinal degeneration.
The renal inflammatory network of nephronophthisis
Authors: Quatredeniers Marceau , Bienaimé Frank , Ferri Giulia , Isnard Pierre , Porée Esther , Billot Katy , Birgy Eléonore , Mazloum Manal , Ceccarelli Salomé , Silbermann Flora , Braeg Simone , Nguyen-Khoa Thao , Salomon Rémi , Gubler Marie-Claire , Kuehn E Wolfgang , Saunier Sophie , Viau Amandine 
Published: January 2022

Abstract
Renal ciliopathies are the leading cause of inherited kidney failure. In autosomal dominant polycystic kidney disease (ADPKD), mutations in the ciliary gene PKD1 lead to the induction of CCL2, which promotes macrophage infiltration in the kidney. Whether or not mutations in genes involved in other renal ciliopathies also lead to immune cells recruitment is controversial. Through the parallel analysis of patients’ derived material and murine models, we investigated the inflammatory components of nephronophthisis (NPH), a rare renal ciliopathy affecting children and adults. Our results show that NPH mutations lead to kidney infiltration by neutrophils, macrophages and T cells. Contrary to ADPKD, this immune cell recruitment does not rely on the induction of CCL2 in mutated cells, which is dispensable for disease progression. Through an unbiased approach, we identified a set of inflammatory cytokines that are upregulated precociously and independently of CCL2 in murine models of NPH. The majority of these transcripts is also upregulated in NPH patient renal cells at a level exceeding those found in common non-immune chronic kidney diseases. This study reveals that inflammation is a central aspect in NPH and delineates a specific set of inflammatory mediators that likely regulates immune cell recruitment in response to NPH genes mutations.
Mathematical modeling of COVID-19 pandemic in the context of sub-Saharan Africa: a short-term forecasting in Cameroon and Gabon
Authors: Nkwayep C H , Bowong S , Tsanou B , Alaoui M A Aziz , Kurths J 
Published: January 2022

AbstractIn this paper, we propose and analyse a compartmental model of COVID-19 to predict and control the outbreak. We first formulate a comprehensive mathematical model for the dynamical transmission of COVID-19 in the context of sub-Saharan Africa. We provide the basic properties of the model and compute the basic reproduction number $\mathcal {R}_0$ when the parameter values are constant. After, assuming continuous measurement of the weekly number of newly COVID-19 detected cases, newly deceased individuals and newly recovered individuals, the Ensemble of Kalman filter (EnKf) approach is used to estimate the unmeasured variables and unknown parameters, which are assumed to be time-dependent using real data of COVID-19. We calibrated the proposed model to fit the weekly data in Cameroon and Gabon before, during and after the lockdown. We present the forecasts of the current pandemic in these countries using the estimated parameter values and the estimated variables as initial conditions. During the estimation period, our findings suggest that $\mathcal {R}_0 \approx 1.8377 $ in Cameroon, while $\mathcal {R}_0 \approx 1.0379$ in Gabon meaning that the disease will not die out without any control measures in theses countries. Also, the number of undetected cases remains high in both countries, which could be the source of the new wave of COVID-19 pandemic. Short-term predictions firstly show that one can use the EnKf to predict the COVID-19 in Sub-Saharan Africa and that the second vague of the COVID-19 pandemic will still increase in the future in Gabon and in Cameroon. A comparison between the basic reproduction number from human individuals $\mathcal {R}_{0h}$ and from the SARS-CoV-2 in the environment $\mathcal {R}_{0v}$ has been done in Cameroon and Gabon. A comparative study during the estimation period shows that the transmissions from the free SARS-CoV-2 in the environment is greater than that from the infected individuals in Cameroon with $\mathcal {R}_{0h}$ = 0.05721 and $\mathcal {R}_{0v}$ = 1.78051. This imply that Cameroonian apply distancing measures between individual more than with the free SARS-CoV-2 in the environment. But, the opposite is observed in Gabon with $\mathcal {R}_{0h}$ = 0.63899 and $\mathcal {R}_{0v}$ = 0.39894. So, it is important to increase the awareness campaigns to reduce contacts from individual to individual in Gabon. However, long-term predictions reveal that the COVID-19 detected cases will play an important role in the spread of the disease. Further, we found that there is a necessity to increase timely the surveillance by using an awareness program and a detection process, and the eradication of the pandemic is highly dependent on the control measures taken by each government.
K-Theoretic Hall Algebras of Quivers with Potential as Hopf Algebras
Authors: Pădurariu Tudor 
Published: January 2022

AbstractPreprojective K-theoretic Hall algebras (KHAs), particular cases of KHAs of quivers with potential, are conjecturally positive halves of the Okounkov–Smirnov affine quantum algebras. It is thus natural to ask whether KHAs of quivers with potential are halves of a quantum group. For a symmetric quiver with potential satisfying a Künneth-type condition, we construct (positive and negative) extensions of its KHA, which are bialgebras. In particular, there are bialgebra extensions of preprojective KHAs and one can construct their Drinfeld double algebra.
Patient-reported outcomes as hospital performance measures: the challenge of confounding and how to handle it
Authors: Kristensen Pia Kjær , Johnsen Søren Paaske 
Published: January 2022

Abstract
It is highly appealing to use patient-reported outcomes (PROs) as hospital performance measures; however, so far, the attention to key methodological issues has been limited. One of the most critical challenges when comparing PRO-based performance measures across providers is to rule out confounding. In this paper, we explain confounding and why it matters when comparing across providers. Using examples from studies, we present potential strategies for dealing with confounding when using PRO data at an aggregated level. We aim to give clinicians an overview of how confounding can be addressed in both the design stage (restriction, matching, self-controlled design and propensity score) and the analysis stage (stratification, standardization and multivariable adjustment, including multilevel analysis) of a study. We also briefly discuss strategies for confounding control when data on important confounders are missing or unavailable.
Implementation of the EUCAST rapid antimicrobial susceptibility test (RAST) directly from positive blood culture bottles without the advanced identification systems
Authors: Tayşi Muhammet Rıdvan , Şentürk Gönül Çiçek , Çalişkan Elif , Öcal Duygu , Miroglu Gülşah , Şencan İrfan 
Published: January 2022

Abstract

Objectives
EUCAST published its recommendations for rapid antimicrobial susceptibility tests (RASTs) directly from positive signal blood culture (BC) bottles. The objective of the present study was to investigate the accuracy and applicability of the predicted RAST (p-RAST) method without using automated identification systems, and the effects of the results obtained with this method on the treatment decision of the clinician.

Methods
The RAST procedure was applied to positive BC samples between November 2020 and June 2021. The categorical results of the method were obtained by comparing the p-RAST results obtained at 4, 6 and 8 h of incubation according to predicted bacterial species with conventional methods and standard disc diffusion results. The effects of these results on the treatment decision of the clinician were evaluated retrospectively. The actual categorical results of the EUCAST RAST [standard RAST (s-RAST)] method were identified.

Results
The p-RAST and s-RAST results were analysed according to 145 and 111 isolates, respectively. The p-RAST total error rates were 3.0%, 3.1% and 2.8% at 4, 6 and 8 h of incubation, respectively, and the s-RAST total error rates were determined as 2.7%, 3.3% and 3.2%, respectively. With p-RAST’s results, it was observed that effective escalation was performed in the antimicrobial treatment for 45 patients, and effective de-escalation could be performed in 32 patients, but it was recommended not to perform de-escalation.

Conclusions
Even in a microbiology laboratory with limited facilities, reliable antimicrobial susceptibility test results can be obtained in a short time with the p-RAST method without using automated systems and antimicrobial choice can be guided in a shorter time.
Effect of topical berberine in murine cutaneous leishmaniasis lesions
Authors: Calvo Alba , Moreno Esther , Aldalur Irati , Sanmartín Carmen , Larrea Esther , González-Peñas Elena , Irache Juan Manuel , Espuelas Socorro 
Published: January 2022

Abstract

Objectives
More effective topical treatments remain an unmet need for the localized forms of cutaneous leishmaniasis (CL). The aim of this study was to evaluate the efficacy and safety of a topical berberine cream in BALB/c mice infected with Leishmania major parasites.

Methods
A cream containing 0.5% berberine-β-glycerophosphate salt and 2.5% menthol was prepared. Its physicochemical and stability properties were determined. The cream was evaluated for its capacity to reduce lesion size and parasitic load as well as to promote wound healing after twice-a-day administration for 35 days. Clinical biochemical profile was used for estimating off-target effects. In vitro time-to-kill curves in L. major-infected macrophages and skin and plasma pharmacokinetics were determined, aiming to establish pharmacokinetic/pharmacodynamic relationships.

Results
The cream was stable at 40°C for 3 months and at 4°C for at least 8 months. It was able to halt lesion progression in all treated mice. At the end of treatment, parasite load in the skin was reduced by 99.9% (4 log) and genes involved in the wound healing process were up-regulated compared with untreated mice.
The observed effects were higher than expected from in vitro time-to-kill kinetic and plasma berberine concentrations, which ranged between 0.07 and 0.22 μM.

Conclusions
The twice-a-day administration of a topical berberine cream was safe, able to stop parasite progression and improved the appearance of skin CL lesions. The relationship between drug plasma levels and in vivo effect was unclear.
Large-scale WGS of carbapenem-resistant Acinetobacter baumannii isolates reveals patterns of dissemination of ST clades associated with antibiotic resistance
Authors: Frenk Sammy , Temkin Elizabeth , Lurie-Weinberger Mor N. , Keren-Paz Alona , Rov Reut , Rakovitsky Nadya , Wullfhart Liat , Nutman Amir , Daikos George L. , Skiada Anna , Durante-Mangoni Emanuele , Dishon Benattar Yael , Bitterman Roni , Yahav Dafna , Daitch Vered , Bernardo Mariano , Iossa Domenico , Zusman Oren , Friberg Lena E. , Mouton Johan W. , Theuretzbacher Ursula , Leibovici Leonard , Geffen Yuval , Gershon Rina , Paul Mical , Carmeli Yehuda 
Published: January 2022

Abstract

Objectives
To describe the population genetics and antibiotic resistance gene distribution of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates causing infections in three Mediterranean countries.

Methods
Isolates were collected during the 2013–17 AIDA clinical trial in six hospitals in Israel, Greece and Italy. WGS, bioinformatic characterization and antibiotic resistance profiling were performed.

Results
In the 247 CRAB isolates characterized in this study, ST distribution varied by country: 29/31 (93.5%) Greek isolates, 34/41 (82.9%) Italian isolates and 70/175 (40.0%) Israeli isolates belonged to ST2. The identified ST2 isolates included eight distinct clades: 2C, 2D and 2H were significantly more common in Italy, while 2F was unique to Greece. The uncommon ST3 was not present among Greek isolates and constituted only 5/41 (12%) Italian isolates. On the other hand, it was much more common among Israeli isolates: 78/175 (44.6%) belonged to ST3. The vast majority of isolates, 240/247 (97.2%), were found to harbour acquired carbapenemases, primarily blaOXA-23. The chromosomal oxaAb (blaOXA-51-like) and ampC genes characteristic of this organism were also ubiquitous. Most (96.4%) ST3 isolates carried a broad-host-range plasmid IncP1α.

Conclusions
The geographical differences in CRAB populations support the theory that clonal spread of CRAB leads to endemicity in hospitals and regions. The close association between antibiotic resistance genes and clades, and between plasmids and STs, suggest that de novo creation of MDR A. baumannii is rare. The clustering of antibiotic resistance genes and plasmids that is unique to each clade/ST, and nearly uniform within clades/STs, suggests that horizontal transmission is rare but crucial to the clade’s/ST’s success.
Research Progress on Preparation, Mechanism, and Clinical Application of Nanofat
Authors: Ding Pengbing , Lu Enhang , Li Guan , Sun Yidan , Yang Wenhui , Zhao Zhenmin 
Published: January 2022

Abstract
Autologous adipose tissue is an ideal soft tissue filling material in theory, which has the advantages of easy access, comprehensive source, and high biocompatibility and is now widely used in clinical practice. Based on the above benefits of autologous fat, autologous fat grafting is an essential technique in plastic surgery. Conventional macrofat is used to improve structural changes after soft tissue damage or loss caused by various causes such as disease, trauma, or aging. Due to the large diameter of particles and to avoid serious complications such as fat embolism, blunt needles with larger diameters (2 mm) are required, making the macrofat grafting difficult to the deep dermis and subdermis. Nanofat grafting is a relatively new technology that has gained popularity in cosmetic surgery in recent years. Nanofat is produced by mechanical shuffling and filtration of microfat, which is harvested by liposuction. The harvesting and processing of nanofat are cost-effective as it does not require additional equipment or culture time. Unlike microfat, nanofat particles are too small to provide a notable volumizing effect. Studies have shown that nanofat contains abundant stromal vascular fraction cells and adipose-derived stem cells, which help reconstruct dermal support structures, such as collagen, and regenerate healthier, younger-looking skin. Moreover, the fluid consistency of nanofat allows application in tissue regeneration, such as scars, chronic wounds, and facial rejuvenation. This article reviews the current research progress on the preparation, mechanism, and clinical application of nanofat.
A topology optimization approach to structure design with self-supporting constraints in additive manufacturing
Authors: Wu Zijun , Xiao Renbin 
Published: January 2022

Abstract
The manufacturability model of complex structure is the premise of manufacturing, and it is necessary to consider material properties, structure design, manufacturing constraints, and so on. However, due to the inconsistent restrictions between design and manufacturing, it is not easy to obtain the manufacturable structure that matches its design performance using layer-wise manufacturing. This paper presents a topology optimization method for manufacturable form, which incorporates the self-supporting factors such as overhang angle and length based on the characteristics of the generic additive manufacturing process. The support relationship between the supporting and supported elements in self-supporting constraints is mapped to a cascade relationship between two adjacent layers. To avoid a low-density structure supporting multiple high-density ingredients, we establish a fabrication model using the smax and smin operators. Also, the sensitivity analysis and variable updating method are given under the Solid Isotropic Material with Penalization method. Furthermore, numerical examples are shown to validate the correctness and superiority of this proposed self-supporting structure design method.