bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of extracorporeal shock wave therapy on large neurogenic heterotopic ossification in a patient with pontine hemorrhage: A case report and literature review
Authors: Kim Youngmin , Lee Sook Joung , Choi Eunseok , Lee Sangjee , Lee Jungsoo , Park Eunjin 
Published: October 2022

Rationale:
Heterotopic ossification (HO), an ectopic bone formation in soft tissue around the joint, is a complication observed in stroke patients. HO around the hip joint causes a reduction in the functional ability of patients by generating pain and limiting range of motion (ROM). In addition, it results in impaired mobility, ultimately affecting quality of life and increasing the mortality of patients. Extracorporeal shock wave therapy (ESWT) has demonstrated efficacy in treating soft tissue inflammation and has been used to reduce patients’ pain in HO. However, almost none of the studies reported degradation in the size of HO on images obtained before and after ESWT application.

Patient concerns and Diagnosis:
We report a case of a 36-year-old man who developed HO around both hip joints 3 months after bilateral pontine hemorrhage.

Interventions:
Seven months after HO development, ESWT was administered to the area of HO every other day for a total of 10 sessions.

Outcomes:
Immediately following treatment, the ROM of both hip joints increased. Thus the patient was able to maintain a sitting posture without having to be bound to the wheelchair. In addition, the tolerable sitting time before groaning increased from less than ten minutes to almost 60 minutes by the end of all ESWT sessions. Unlike other previous reports, a diminished HO size was confirmed by comparing plain X-rays and bone scans obtained before and after treatment sessions.

Lessons:
In this case, we report an objective size reduction in HO in radiologic findings after applying ESWT to both hips. ESWT is a safe, easy-to-apply, and noninvasive modality. We would like to emphasize the use of ESWT as a treatment option for HO to decrease the extent of HO, as well as to improve pain, spasticity and function in patients with stroke.
Challenging Metastatic Melanoma to the Lung With p40 Expression: A Diagnostic Pitfall, Case Report
Authors: Thierry Sixte , Juberthie Bernard , Gyenes Cezar , Dramais-Marcel Dominique , Forest Fabien 
Published: October 2022

p40 immunohistochemistry is a cornerstone of histopathological examination for non-small cell lung carcinoma. p40 is an isoform of p63 and is reported to be highly specific for the diagnosis of squamous cell carcinoma. Very rare pitfalls are reported for this antibody, and p40 is typically negative in melanoma. A 66-year-old patient was admitted for multiple hemorrhagic brain tumors evocative of secondary tumors. On imaging, a 26 mm lung tumor was detected, and a biopsy of the lung tumor was performed. The tumor was stained by melanic markers and diffusely stained by p40 and p63. Molecular analysis found a somatic p.Asn581Ser (c.1742A>G) point mutation in exon 15 of BRAF and a p.Arg80Ter (c.238C>T) germline variant of CDKN2A, a predisposing mutation to melanoma. This case report highlights the importance of clinical, pathologic, and molecular correlation.
Design and Use of a Pair of Computer-Designed Surgical Templates in Reduction of a Comminuted Zygomatic Arch Fracture
Authors: Lin Yuxin , Zhou Haihua , Lv Kun , Li Zhi , Li Zubing 
Published: October 2022

Background:
Isolated depressed zygomatic arch fractures are often treated with closed reduction. Reduction is usually performed through the Gillies approach or Keen approach. comminuted zygomatic arch fractures generally require open reduction and fixation to achieve good results. This article describes how to use a pair of surgical templates to assist in the fixation of comminuted zygomatic arch fractures with absorbable plates.

Methods:
A pair of computer-designed surgical templates were applied to restore the main part of zygomatic arch. Placing a surgical template on the medial side of the zygomatic arch can provide a supporting force and improves the stability of the reduced bone fragments. The lateral template of zygomatic arch limits the excessive uplift of bone fragments.

Results and Discussion:
The operation was performed according to the predetermined procedure. Postoperative computed tomography showed satisfactory reduction effect. In conclusion, with the aid of surgical templates, the reduction and fixation of comminuted zygomatic arch fractures can be more easily performed using absorbable plates.
Total Resection of Cerebellopontine Angle Meningioma via Presigmoid Transmastoid Approach: An Otologist’s Perspective
Authors: Guo Fang , Zhang De-Jun , Fu Ze-Ming , Guo Ying-Yuan , Wan Yi-Ning , Tang Na , Guan Guo-Fang 
Published: October 2022

Purpose:
Cerebellopontine angle meningiomas (CPAMs) are benign tumors that arise from the dura mater of the petrosal surface of the temporal bone, lateral to the trigeminal nerve. This study aimed to describe 1 case of CPAMs violating the mastoid and highlight the unique superiority of the presigmoid transmastoid approach for this type of CPAMs from an otologist’s perspective.

Methods:
One case of specific CPAMs treated by total resection via presigmoid transmastoid approach in otomicrosurgery was described.

Results:
A patient was referred for the left intracranial space-occupying lesion found in physical examination. Surgical resection via presigmoid transmastoid approach was performed and there was no sign of recurrence of tumor 2 years after the operation.

Conclusions:
Presigmoid transmastoid approach in otomicrosurgery is suitable for CPAMs invading the mastoid. It is suggested that neurosurgeons and ear surgeons should comprehensively analyze the type and extent of the tumor and flexibly adopt surgical methods to ensure it is the best for patients.
Basement Membrane Changes of Myofiber and Fibrosis in Sternocleidomastoid Muscle of Congenital Muscular Torticollis
Authors: Gao Futang , Zeng Shuaidan , Tang Shengping , Ning Pei , Qiu Xin , Cai Ting , Tang Gen , Xiong Zhu 
Published: October 2022

Objective:
To investigate the correlation between the features of basement membranes (BMs) of residual myofibers in the sternocleidomastoid muscle (SCM) and fibrosis of congenital muscular torticollis (CMT) in children, and to evaluate the relationship between BM changes and appearance of CMT.

Materials and Methods:
We reviewed the CMT patients from 2017 to 2018 and performed pathological studies. Forty resected specimens were stained by hematoxylin and eosin and Masson collagen staining. Immunohistochemical staining of collagen IV and laminin was also performed. Five adductor muscle specimens from patients with developmental dysplasia of the hip were used as the control group.

Results:
Hematoxylin and eosin staining revealed apparent interstitial fibrosis around residual myofibers in lesion specimens. However, the severity of fibrosis differed within the same samples. The average percent area of fibrous tissue in affected SCMs and controls were different significantly. Immunohistochemical staining of collagen IV and laminin showed these proteins were mostly expressed in the BM and vascular wall of affected SCM. However, BMs and myofibers from three different areas within the same SCM tissue exhibited significant differences in proteins expression.

Conclusions:
Therefore, the defective BMs are associated with myofiber and mesenchyme fibrosis in patients with CMT, which is crucial for understanding the histopathology of SCM.
Long-Term Effect of Intermediate Rhinoplasty in Unilateral Complete Cleft Lip Nasal Deformity
Authors: Park Joseph Kyu-hyung , Koo Hee Tae , Choi Kyeong Beom , Kim Sukwha , Chung Jee Hyeok 
Published: October 2022

Background:
Intermediate rhinoplasties are performed during preschool to reduce the patients’ psychosocial burden. At our institution, limited dissection of the cartilages followed by suspension and interdomal sutures was performed through an alar rim incision on the cleft side to minimize the risk of iatrogenic nasal growth restriction. However, the long-term outcomes of “limited intermediate rhinoplasty” through skeletal growth are uncertain.

Materials and Methods:
A retrospective review of all unilateral complete cleft lip and/or palate patients who underwent definitive rhinoplasty was performed. To avoid the confounding effect of primary rhinoplasty, only the patients who did not receive primary rhinoplasty were included in the analysis. The maneuvers performed during definitive rhinoplasty were analyzed and compared between patients who underwent intermediate rhinoplasty and those who did not.

Results:
A total of 60 Korean patients (27 female and 33 male) underwent definitive rhinoplasty at the average age of 20.6 years old (17.1–25.5). Forty-three (71.6%) patients previously underwent intermediate rhinoplasty. A combination of 6 maneuvers was performed based on the deformity of each subunit (alar medialization, interdomal with suspension sutures, nostril sill depression correction, septoplasty, osteotomy, and hump rasping). The average number of maneuvers performed during definitive rhinoplasty was significantly higher in the intermediate group (3.31 versus 2.1, P=0.012). Alar medialization and nostril sill depression correction were more frequently performed in the intermediate group, while the frequencies of other maneuvers were not statistically different.

Conclusion:
While intermediate rhinoplasty improves the patients’ psychosocial well-being, the effects of “limited intermediate rhinoplasty” manipulating only the cartilages do not seem to last until skeletal maturity. A more comprehensive dissection allowing the release of the lower lateral cartilage in the hinge area along with septoplasty may be more effective in providing longer-lasting effects.
Application of a Multiplicative Cascade Model to Detect the Early Signs of SARS-CoV-2 Infection Using Heart Rate Data
Authors: Heath Rachel 
Published: October 2022

AbstractBackgroundWrist-worn devices can keep track of a person’s daily health status, including those likely to become infected with the SARS-CoV-2 virus. Technological solutions using mobile devices are being developed to predict the time course of COVID-19.ObjectiveIn this proof-of-concept study, we use heart rate data to detect the first sign of infection in people who have been diagnosed with COVID-19 and to monitor the time-course of the illness.MethodsThe heart-rate data were analysed using a multiplicative cascade driven by a Gaussian process. This provides two parameters, mean and standard deviation, which when combined with similar parameters estimated from control series, provide a Health Index.ResultsFor 90% of 31 cases, the Health Index tracked COVID-19 infection with the virus and subsequent recovery. The first-sign of COVID-19 was detected on average nine days before symptoms were reported.ConclusionsEarly detection of COVID-19 may lead to a reduction in the spread of the virus. The Heath Index’s potential use for the early detection of complications arising from Long COVID would be an important innovation.
Transcription, structure, and organoids translate time across the lifespan of humans and great apes
Authors: Charvet Christine J. , Ofori Kwadwo , Falcone Carmen , Rigby-Dames Brier A. 
Published: October 2022

AbstractHow the neural structures supporting human cognition develop and arose in evolution is an enduring question of interest. Yet, we still lack appropriate procedures to align ages across primates, and this lacuna has hindered progress in understanding the evolution of biological programs. We generated a dataset of unprecedented size consisting of 573 time points from abrupt and gradual changes in behavior, anatomy, and transcription across human and 8 non-human primate species. We included time points from diverse human populations to capture within-species variation in the generation of cross-species age alignments. We also extracted corresponding ages from organoids. The identification of corresponding ages across the lifespan of 8 primate species, including apes (e.g., orangutans, gorillas) and monkeys (i.e., marmosets, macaques) reveal that some biological pathways are extended in humans compared with some non-human primates. Particularly, the human lifespan is unusually extended relative to studied nonhuman primates demonstrating that very old age is a phase of life in humans that does not map to other studied primate species. More generally, our work prompts a reevaluation in the choice of a model system to understand aging given very old age in humans is a period of life with a clear counterpart in great apes.Significance StatementWhat is special about the duration of human development and aging has been an enduring source of interest. A significant hurdle in identifying which biological programs are unusually extended in humans is the lack of standardized approaches with which to align ages across species. We harnessed temporal variation in behavior, transcription, and anatomy to align ages across the lifespan of primates. These data reveal which biological programs are conserved, and which are modified. Harnessing time points across scales of study guides the choice of model systems to understand disease progression, and can be used to enhance care of great apes, many of which are critically endangered.
Chromosome-level genome assembly ofTorreya grandisprovides insights into the origin and evolution of gymnosperm-specific sciadonic acid biosynthesis
Authors: Lou Heqiang , Song Lili , Li Xiaolong , Chen Weijie , Gao Yadi , Zheng Shan , Fei Zhangjun , Sun Xuepeng , Wu Jiasheng 
Published: October 2022

AbstractSpecies in genusTorreyaare nut trees that produce dry fruits with a wide assortment of functions. Here, we report the 19-Gb chromosome-level genome assembly ofT. grandis.The genome is shaped by an ancient whole genome duplication and recurrent LTR retrotransposon bursts. Comparative genomic analyses reveal key genes involved in reproductive organ development, cell wall biosynthesis and seed storage. Two genes encoding a C18Δ9-elongase and a C20Δ5-desaturase are identified inT. grandisto be responsible for sciadonic acid biosynthesis and both are present in diverse plant lineages except angiosperms. We demonstrate that the histidine-rich boxes of the Δ5-desaturase are crucial for its catalytic activity. Methylome analysis reveals that methylation valleys of theT. grandisseed genome harbor genes associated with important seed activities, including cell wall and lipid biosynthesis. Moreover, seed development is accompanied by DNA methylation changes that possibly fuel energy production. This study provides important genomic resource for gymnosperms and unravels key enzymes for biosynthesis of sciadonic acid as a hallmark metabolite of gymnosperms.
Genome-wide association study meta-analysis supports association between MUC1 and ectopic pregnancy
Authors: Pujol Gualdo Natàlia ,   Estonian Biobank Research Team, Mägi Reedik , Laisk Triin 
Published: October 2022

AbstractEctopic pregnancy is an important cause of maternal morbidity and mortality worldwide. To better understand the genetic risk factors underlying this pregnancy complication, we conduct a GWAS meta-analysis and identify two genome-wide significant loci on chromosomes 1 (rs4971091, p=5.32×10−9) and 10 (rs11598956, p=2.41×10−8). Follow-up analyses propose MUC1, an epithelial glycoprotein with an important role in barrier function, as the most likely candidate for the association on chromosome 1. We also characterise the phenotypic and genetic correlations with other phenotypes, identifying a genetic correlation with smoking and diseases of the (genito)urinary and gastrointestinal system, and phenotypic correlations with various reproductive health diagnoses, reflecting the previously known epidemiological associations.
The extracellular matrix differentially directs myoblast motility in distinct forms of muscular dystrophy
Authors: Long Ashlee M. , Kwon Jason M. , Lee GaHyun , Reiser Nina. L. , Vaught Lauren. A , O’Brien Joseph G. , Page Patrick G.T. , Hadhazy Michele , Reynolds Joseph C. , Crosbie Rachelle H. , Demonbreun Alexis R. , McNally Elizabeth M. 
Published: October 2022

ABSTRACTExtracellular matrix (ECM) pathologic remodeling underlies many fibrotic disorders, including muscular dystrophy. Tissue decellularization removes cellular components while leaving behind ECM components. We generated “on-slide” decellularized tissue slices from genetically distinct dystrophic mouse models. The ECM of dystrophin-and sarcoglycan-deficient muscles had marked thrombospondin 4 deposition, while dysferlin-deficient muscle had excess decorin. Annexins A2 and A6 were present on all dystrophic decellularized ECMs, but annexin matrix deposition was excessive in dysferlin-deficient muscular dystrophy. Adeno-associated viral expression of annexin A6 specifically in muscle resulted in annexin A6 deposition throughout the ECM, indicating muscle as a source of this ECM protein. C2C12 myoblasts seeded onto decellularized matrices displayed differential myoblast mobility. Dystrophin-deficient decellularized matrices inhibited myoblast mobility while dysferlin-deficient decellularized matrices enhanced myoblast movement. Myoblasts treated with recombinant annexin A6 increased mobillity similar to that seen on dysferlin-deficient decellularized matrix. These findings demonstrate specific fibrotic signatures elicit effects on myoblast activity.TEASERFibrosis in muscular dystrophy has differential effects on myoblastsHIGHLIGHTSSpatial architecture and composition of the ECM differ across genetically distinct forms of muscular dystrophy, especially with respect to Annexin A6 protein depositionMatrix from dystrophin-mediated muscular dystrophy inhibits myoblast movementMatrix from dysferlin-deficient muscular dystrophy promotes myoblast motilityAnnexin A6 was sufficient to enhance myoblast motility
Lipid peroxidation increases membrane tension, Piezo1 gating and cation permeability to execute ferroptosis
Authors: Hirata Yusuke , Cai Ruiqi , Volchuk Allen , Steinberg Benjamin E. , Matsuzawa Atsushi , Grinstein Sergio , Freeman Spencer A. 
Published: October 2022

AbstractThe ongoing metabolic and microbicidal pathways that support and protect cellular life generate potentially damaging reactive oxygen species (ROS). To counteract damage, cells express peroxidases, antioxidant enzymes that catalyze the reduction of oxidized biomolecules. Glutathione peroxidase 4 (GPX4) is the major hydroperoxidase specifically responsible for reducing lipid peroxides; this homeostatic mechanism is essential and its inhibition causes a unique type of lytic cell death, ferroptosis. The mechanism(s) that lead to cell lysis in ferroptosis, however, are unclear. We report that the lipid peroxides formed during ferroptosis accumulate preferentially at the plasma membrane. Oxidation of surface membrane lipids increased tension on the plasma membrane and led to the activation of Piezo1 and TRP channels. Oxidized membranes thus became permeable to cations, ultimately leading to gain of cellular Na+and Ca2+concomitant with loss of K+. These effects were reduced by deletion of Piezo1 and completely inhibited by blocking cation channel conductance with ruthenium red or 2-aminoethoxydiphenyl borate (2-APB). We also found that the oxidation of lipids depressed the activity of the Na+/K+-ATPase, exacerbating the dissipation of monovalent cation gradients. Preventing the changes in cation content attenuated ferroptosis. Together, our study establishes that increased membrane permeability to cations is a critical step in the execution of ferroptosis and identifies Piezo1, TRP channels and the Na+/K+-ATPase as targets/effectors of this type of cell death.
Pulling back the curtain of environmental accountability: How boundaries shape environmental identities in the SKI industry
Authors: Gamble Edward , Caton Gary 
Published: October 2022

PurposeThis paper aims to explore the important role boundaries play in back-office framing of environmental engagement. This is of particular interest because it is not clear how organizations in an industry without standardized environmental reporting navigate their boundaries behind the scenes and why they engage with the environment the way they do. This element of their environmental identity offers important insights into the emergence of sustainability reporting.Design/methodology/approachGuided by Miles and Ringham (2019) the authors conduct an ethnography of the Montana ski industry. The ethnography includes extensive on-site observations at nine Montana ski areas and interviews with 16 ski area executives, two regulators and a land development executive.FindingsThe authors find three key boundaries – accountability structure, degree of regulatory burden and impact measurement approach – that shape the back-office economic and environmental framing of ski executives (Goffman, 1959, 1974). From these back-office frames the authors identify four front-office cultural performances – community ecosystem, quantitative ownership, approval seeking and advocacy platform – that represent the environmental engagement strategies at these resorts.Practical implicationsUnderstanding the relationships between boundaries and environmental engagement is an important step in developing appropriate industry-wide environmental accountability and sustainability expectations. The study’s findings extend to other industries that are both highly dependent on the environment and are in the early stages of developing environmental reporting standards.Originality/valueSki resorts operate in an industry that is impacted by changes in the natural environment. The authors chronicle the process by which boundaries lead to framing which leads to environmental engagement in this weather-dependent industry. The authors explain the process of environmental identity building, the result of which both precedes environmental reporting and puts such reporting into context. In this sense, the authors show how boundaries are set and maintained in the ski resort industry, and how fundamental these boundaries are to the development of individual companies' environmental engagement strategies.