bluuebunny/crossref_metadata_2025_split huggingface dataset:
The red seaweed Asparagopsis taxiformis genome and integrative -omics analysis
Authors: Zhao M , Campbell AH , ZP Patwary , Wang T , Lang T , Webb J , GC Zuccarello , Wegner A , Heyne D , McKinnie L , Pascelli C , Satoh N , Shoguchi E , NA Paul , SF Cummins 
Published: November 2022

Abstract
Seaweeds (marine macroalgae) are crucial to the functioning of healthy coastal ecosystems and global biogeochemical cycles, and sometimes provide novel solutions to help mitigate climate change. The red seaweed Asparagopsis taxiformis (Bonnemaisoniaceae, Rhodophyta) produces bioactive natural products that, when fed to cattle and sheep, can eradicate methane emissions from these livestock. However, in order to cultivate enough A. taxiformis to have a meaningful impact on global greenhouse gas emissions, we need to improve our understanding of the biology of this new crop. In this study, we used a domesticated diploid sporophyte (> 1.5 years in culture, with relatively low microbial diversity) to establish a high-quality draft nuclear genome for A. taxiformis from Queensland, Australia. The A. taxiformis lineage was confirmed as Lineage 6 (L6) based upon phylogenetic analysis (Cox2-3 spacer). The genome of A. taxiformis (L6) was 142 Mb in size with approximately 11,000 protein-coding genes, including those associated with secondary metabolism, photosynthesis and defence, and the assembly contained 70.67% repeat regions. Based on protein domain analysis, the most prominent lineage-specific duplications belonged to those containing WD repeat proteins, as well as bestrophin and N6_N4_Mtase domain proteins. Cultured (domesticated) A. taxiformis (L6) sporophytes contained 4-times more bromoform (the key anti-methanogenic natural product) compared to wild sporophytes. To obtain information regarding associated molecular differences, the genome was used as a reference to explore differential gene expression related to environment. Cultured sporophytes demonstrated an enrichment of regulatory factors (kinases, transcription factors), whereas wild sporophytes were enriched with defence and stress-related genes, including those involved in protein folding (heat shock proteins) and halogenated metabolite production. Wild sporophytes also expressed a relatively high level of novel secreted proteins, with similarity to collagen-alpha proteins (termed rhodophyte collagen-alpha-like proteins, RCAPs). Proteomic investigation of the genome of cultured sporophytes, resulting in the identification of over 400 proteins, including RCAPs, as well as numerous enzymes and phycobiliproteins, which will facilitate future functional characterisation. In summary, as the most comprehensive genomic resource for any Asparagopsis species, this resource provides a gateway for seaweed researchers to fast-track the development and production of Asparagopsis to meet demand by agriculture and do so with economic and environmental agility.
A Novel Prognostic Risk Model for Necroptosis-associated Genes in Acute Lymphoblastic Leukemia
Authors: Liu Yueyao , Gou Lin , Ma Zhi-gui 
Published: November 2022

Abstract
Background
Acute lymphoblastic leukemia (ALL) is a hematological malignancy with high heterogeneity that develops in both children and adults. Necroptosis is a newly recognized form of cell death. Nevertheless, the prognostic significance and functions of necroptosis-related genes (NRGs) in ALL have not received sufficient attention. This study aimed to evaluate the association between NRGs levels and ALL prognosis using publicly available datasets.
Methods
RNA-sequencing (RNA-seq) data and clinical information for ALL patients were obtained from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database. Univariate Cox and LASSO regression analyses were applied to select prognosis-related genes for the construction of a prognostic risk-scoring model. Internal and external validation proved the model efficient. Furthermore, we explored the correlation of the prognostic risk model with immune infiltration and drug resistance.
Results
A total of 7 necroptosis-related genes (NRGs), namely BIRC2, PKP3, MERTK, KL, ESR2, TLE6, and TET2, were used to construct a survival prognostic model. The model exhibited excellent performance in the TARGET cohort and validation group and had good prediction accuracy in screening out high-risk ALL patients. Functional analysis showed that tumor immune microenvironment and drug resistance differed between high- and low-risk groups.
Conclusion
Our NRGs risk score model is a promising strategy for the prediction of ALL patients’ prognosis.
Planting density is a key driver of sorghum yield gaps and variability in smallholder farms of northern Nigeria: Results of an integrated panel survey and crop cutting experiment
Authors: Adam Adam Muhammad , Jibrin Jibrin Mohammed , Shehu Bello Muhammad , Samndi Ayuba Musa , Mustapha Aishat Ayobami , Ajeigbe Hakeem Ayinde , Akinseye Folorunso M. 
Published: November 2022

Abstract
Understanding the drivers of crop yields and variability is fundamental to closing yield gaps and ensuring global food security, especially in smallholder farming systems. This study identified the major socio-economic, soil and crop management factors contributing to sorghum yield variability and yield gaps in the sorghum belt of northern Nigeria. Data on socio-economic indicators, agronomic practices, soil fertility characteristics and yields were collected through integrated agronomic panel and crop cut surveys. These surveys were conducted in two agroecological zones (i.e., Sudan and Sahel savanna zones), the sorghum production belt in Nigeria, among 144 sorghum-producing households using a multistage balanced acceptance sampling procedure. Our results showed that sorghum productivity was relatively higher (43% more) in Sudan compared to the Sahel agroecological zone. Additionally, planting density was the main factor in reducing sorghum productivity across the two zones, with a 68% and 40% yield reduction for the Sudan and Sahel Savanna agroecological zones, respectively. This suggests innovative technologies that promote optimal planting density should be encouraged in farmer extension services to close the persistent sorghum yield gaps under smallholder farming conditions in the two agroecological zones.
Relations between state discrimination and state exclusion
Authors: Chen Jun , Qiao XIao-Yan 
Published: November 2022

Abstract
Consider a quantum system prepared in a state chosen from a known set, the aim of quantum state discrimination is to perform a measurement on the system which can determine that a subset of the possible preparation procedures have been prepared. However, the aim of quantum state exclusion is to perform a measurement on the system which can conclusively rule that a subset of the possible preparation procedures have not taken place. For classical states, probabilities of error in discrimination and exclusion are always equal. For quantum states, we point out that probabilities of error in discrimination and exclusion are still equal in two-state ensembles, however they are no longer equal in multiple-state ensembles. More specifically, the error-probability of discrimination is not less than the error-probability of exclusion. We also show that the error-probability of exclusion is continuous which is similar with the error-probability of discrimination.
A novel home-use culture mechanism for identifying microbial load in urine samples
Authors: Pattnaik Siddharth , Singh Monika , Sahai Riya 
Published: November 2022

Abstract
Diagnosing a urinary tract infection (UTI) is typically a clinical procedure involving multiple steps. The need to perform a test depends on the presence of relevant symptoms. Given the current pandemic situation, visiting a clinic may not be a preferable choice for many users. Many vulnerable groups of patients, namely, males with certain predispositions and pregnant women, may not present with symptoms of UTI, which could give rise to a more complicated situation. Microbial culture provides a definitive diagnosis for the presence of an infection. A home-use culture kit can serve this purpose; however, to our knowledge, no such kit exists. Here, we present a feasibility study of an affordable and easy-to-use home-based setup for quantifying bacterial load in a urine. Teaser A home-use system for monitoring progression and management of UTIs.
Categorizing Molecular Features Of Venom Toxins Using Bioinformatics Tools.
Authors: Shinde Pratiksha 
Published: November 2022

Abstract
Poisonous organisms are represented in many taxa, including kingdom Animalia. During evolution, animals have
developed special organs for production and injection of venoms. Animal venoms are complex mixtures,
compositions of which depend on species producing venom. The most known and studied poisonous terrestrial
animals are snakes, scorpions and snails. Venomous animals produce a myriad of important pharmacological
components. The individual components, or venoms (toxins), are used in ion channel and receptor studies, drug
discovery, and formulation of insecticides. Knowing the key clinical applications of venom, the present investigation
was carried out to understand the molecular basis of venom toxins of animals like snake (L-amino acid oxidases),
cone snail (Contulakin-G) and scorpion (Chlorotoxin) by retrieving the protein sequence information, deducing
various physicochemical properties, predicting secondary structural elements, homology modelling and depicting
the potent antigenic regions using various bioinformatics tools and soft-wares. Because of their remarkable
molecular diversity, venoms are key, albeit challenging, resource for pharmacological discovery that contribute to
the development of drugs that act as anti-tumor agents, heart stimulants and therapies for neurological diseases.
Venom-informatics is a systematic bioinformatics approach in which classified, consolidated and cleaned venom
data are stored into repositories and integrated with advanced bioinformatics tools for the analysis of structure and
function of toxins. Venom-informatics complements experimental studies and helps reduce the number of essential
experiments.
Inhibition of the membrane repair protein annexin-A2 prevents tumour invasion and metastasis
Authors: Gounou Céline , Bouvet Flora , d'Agata Léna , Derieppe Marie-Alix , Rouyer Lucile , Bouton Léa , Mélane Mailys , Chapeau Dorian , Harté Etienne , Martineau Julie , Prouzet-Mauleon Valerie , Tan Sisareuth , Souleyreau Wilfried , Saltel Frederic , Argoul Francoise , Siegfried Geraldine , Khatib Abdel-Majid , Brisson Alain , Iggo Richard , Bouter Anthony 
Published: November 2022

Abstract
Cancer cells are exposed to major compressive and shearing forces during invasion and metastasis, leading to extensive plasma membrane damage. To survive this mechanical stress, they need to repair membrane injury efficiently. Targeting the membrane repair machinery is thus potentially a new way to prevent invasion and metastasis. We show here that annexin-A2 (ANXA2) is required for membrane repair in MDA-MB-231 cells, a highly invasive triple-negative breast cancer cell line. Mechanistically, we show by fluorescence and electron microscopy that cells fail to reseal membrane damaged by shear stress when ANXA2 is silenced or the protein is inhibited with neutralizing antibody. Silencing of ANXA2 has no effect on proliferation in vitro, and even accelerates migration in wound healing assays, but reduces tumor cell dissemination in both mice and zebrafish. We show that high expression of ANXA2 predicts poor prognosis in high-grade lung, ovarian, gastric and breast cancers. We expect that inhibiting membrane repair will be particularly effective in these aggressive, poor prognosis tumors because they rely on the membrane repair machinery to survive membrane damage during tumor invasion and metastasis. This could be achieved either with monoclonal anti-ANXA2 antibodies, which have been shown to inhibit metastasis of MDA-MB-231 cells, or with small molecule drugs.
Strategy for Procalcitonin-Controlled Antimicrobial Therapy in the COVID-19 Pandemic
Authors: Zaytsev A. A. , Dronov I. A. , Kondratieva T. V. 
Published: November 2022

The objective of this review is to optimize the use of antibiotic therapy for COVID-19 based on the published and our own data. The decision to prescribe antibiotic therapy in case of secondary bacterial pneumonia associated with SARS-CoV-2 should be based on a comprehensive assessment of the results of clinical, laboratory and instrumental examination including the elevated level of procalcitonin (more than 0,5 ng/ml).To achieve this objective, 48 publications were analyzed.
Bioleached laterite nano iron catalyst (BLaNFeCs)-based Fenton's degradation of selective dyes in water
Authors: Shivaswamy Bhaskar , Manu Basavaraj , Sreenivasa M. Y. 
Published: November 2022

Abstract
Iron nanocatalyst for its potential application as Fenton's catalyst for the degradation of methylene blue dye was synthesized with the fruit extract of Citrus maxima using bioleached laterite iron as a precursor. Synthesized iron particles were characterized suitably and their catalytic role in the degradation of methylene blue and rhodamine B by Fenton's oxidation was evaluated. The synthesized nanocatalyst exhibits heterogeneous catalytic properties in the degradation of methylene blue and rhodamine B with a degradation efficiency of 93.6 and 91.3%, respectively. Observed rate constants are consistent with the increase in catalyst dosage as it speeds up the reaction. The degradation of methylene blue and rhodamine B follows a pseudo-first-order reaction with a linear fit. Reusability studies confirm the reduction in the catalytic efficiency of the synthesized iron nanoparticles after five consecutive cycles.
Gonopterodendron sarmientoi Lorentz ex Griseb (Palo santo): evaluación del crecimiento para fines maderables
Authors: Chifarelli Vanina , Senilliani, María Gracia , Longhi-Santos Tomaz , Moglia Juana Graciela , Gimenez Ana María 
Published: November 2022

Gonopterodendron sarmientoi Lorentz ex Griseb (Zigophyllaceae) es una especie endémica del noroeste argentino, incluida en el Apéndice II de la Convención Internacional sobre el Tráfico de Especies Amenazadas de Fauna y Flora Silvestres. Esta investigación busca determinar el ciclo de corta basado en la edad de Gonopterodendron sarmientoi, estimada a partir de la medición de los anillos de crecimiento, aplicando una ecuación de crecimiento de diámetro según el sitio. Se trabajo´en 4 sitios según gradiente climatatico de precipitación, seleccionando 35 árboles dado sus limtaciones y restricciones en cuanto su extracción contempadas por la ley. La ecuación general y la obtenida por sitios (Las Lomitas, Ingeniero G. Juárez, Las Hacheras y Rivadavia), se ajustaron por regresión con modelos lineales mixtos. Las Lomitas es la localidad de mayor incremento anual con un espesor promedio de 3.4 mm, seguido por Las Hacheras 3.08 mm, Rivadavia 2.68 mm e Ingeniero G. Juárez 2.19 mm. El espesor medio obtenido es de 3,03 mm. Las edades de corte encontradas en orden decreciente fueron Las Lomitas con 81 años, Rivadavia con 59 años, Ingeniero G. Juárez con 52 años y Las Hacheras con 41 años. Se calculó una rotación promedio de acuerdo con todas las localidades en el rango de 41 años con un tiempo de tránsito de 12 años. A partir de establecer el turno de corta y el tiempo de rotación de una especie amenazada, se podrá lograr un manejo forestal sostenible y sustentable.
PROSES KREATIF TRANSFORMASI WADIAN DADAS PADA RITUAL PENYEMBUHAN DAYAK MA’ANYAN KE BENTUK KARYA TARI HYANG DADAS
Authors: Ramadani Rifkyanoor 
Published: November 2022

Dayak merupakan sebuah julukan kolektif yang diberikan oleh orang barat terhadap suku bangsa pribumi yang menghuni pulau Kalimantan (Borneo). Julukan tersebut terbentuk akibat adanya kesamaan tatanan sosial, aturan norma, adat istiadat, hingga pemukiman yang berbentuk rumah panjang. Dayak Ma’anyan merupakan salah satu sub suku Dayak ot danum tertua yang persebarannya di wilayah tengah dan selatan pulau kalimantan. Sama dengan suku bangsa lainnya, Dayak Ma’anyan juga mempunyai tatanan hidup bermasyarakat, kaidah norma adat, serta kebiasaan mereka sendiri yang sudah terikat dari zaman nenek moyang mereka. Masyarakat suku Dayak Ma’anyan tergolong sebagai masyarakat tradisi lisan, yang mana tradisi lisan pada masyarakat ini menjadi poros utama serta center dalam berkehidupan sosial. Upacara Ritus Balian Dadas merupakan sebuah ritual penyembuhan yang menjadi kebiasaan serta adat yang berkembang di lingkup Dayak Ma’anyan. Sebuah upacara yang mengkaitkan hubungan alam sadar dan alam ghaib menjadikan Ritual Balian Dadas sebagai salah satu cara untuk penyembuhan orang yang sedang sakit.Metode yang dilakukan pada penilitian ini ialah deskriptif kualitatif. Sebuah penelitian yang bersifat naturalistik untuk menangkat fenomena yang terjadi di lingkup sosial bermasyakat. Instrumen yang digunakan pada penelitian ini ialah peneliti sendiri. adapun sumber data pada penelitian ini ialah seorang kereografer karya tari hyang dadas, dan beberapa penari karya tari sersebut untuk melihat berbagai perspektif yang dapat ditarik simpulannya. Teknik yang digunakan pada penilitian ini ialah observasi, wawancara, serta dokumentasi.Seiring perubahan zaman ritual Balian Dadas dikembang menjadi sebuah karya seni pertunjukan sebagai salah satu bagian promosi parawisata daerah. Hyang Dadas merupakan salah satu contoh bentuk karya tari bersifat pertunjukan yang bercermin dan berimplementasi pada ritual Balian Dadas. Menggunakan penari laki-laki yang bertranformasi memiliki unsur feminisme dalam penyajian karya tari ini, membuat berbagai pandangan/persfektif berbeda, dan mempunyai eksistensi atau fenomenal bagi masyarakat Kalimantan tengah serta beberapa seniman di Indonesia. Kata Kunci: Ritual Penyembuhan, Dayak Ma’anyan, Hyang Dadas
Corn silage, a systematic review of the quality and yield in different regions around the world
Authors: García-Chávez Isidro , Meraz-Romero Edgar , Castelán-Ortega Octavio , Zaragoza-Esparza Joob , Osorio-Avalos Jorge , Robles-Jiménez Lizbeth Esmeralda , González-Ronquillo Manuel 
Published: November 2022

Corn sialge (Zea mays L.) is the most widely used in the world in dairy cattle diets because of its higher biomass yields, palatability, homogeneous quality at harvest, and ease of silage due to its higher soluble sugar content. Studies were systematically searched to know the silage corn quality and yield from different countries concerning dry matter yield (t/ha), population density (plant density/ha), quantity of Dry Matter (DM), Crude Protein (CP), Neutral Detergent Fiber (NDF), Non-Fibrous Carbohydrates (NFC), Organic Matter (OM), DM Digestibility (DMD), Neutral Detergent Fiber Digestibility (NDFD), and milk production (kg) per hectare (kg milk/ha) using MILK2006®. Six clusters (CL, PROC CLUSTER) of corn silage were analyzed: CL1: “Starch,” which included DMD, DNDF, TDN1 x DM, Mega calories per kg of DM, and kg of milk/t DM; CL2: “ether extract” which included TDN1 x DM, Mcal/kg DM, and kg of milk/t DM; CL3: “DM,” composed only of DNDF; CL4: “Plant Density,” including Yield of DM/ha and Milk Production/ha; CL5: “NDF,” and CL6: “CP.” In conclusion, CL1 is characterized by a higher DMD, NDFD, CNF, and starch, which allow higher TDN1 x DM and an energy concentration (Mcal/kg DM) with higher milk production (kg milk/ha). CL2 with a higher ether extract (EE) allows higher TDN1 x DM and an energy concentration (Mcal/kg DM) having a higher milk production (kg milk/ha). The DM concentration increases in the whole plant because of maturity, affecting the starch content and NDFD; the DM content (> 35 g/100 g DM) mainly causes a decrease in the NDFD, while in a very early stage of DM harvest (< 25 g/100 g DM) a low ratio of starch:NDFD is present.
Vitamina D en gestantes de zonas extremas de latitud 40 SUR-NORTE: Una revisión para la matronería.
Authors: Urriola-Iturriaga Macarena , Hernozo-Tenore María José , Padilla-Cabezas Paulina , Vergara-Maldonado Cynthia 
Published: November 2022

El objetivo de esta investigación es analizar la deficiencia y la suplementación sugerida de vitamina D en la salud perinatal de gestantes residentes en zonas geográficas de latitud40°sur-norte según revisiones de las últimas tres décadas y relacionarlas con el rol de matronas(es).
La metodología utilizada fue una revisión sistemática cualitativa de estudios de texto completo, realizados en zonas geográficas mayores al paralelo 40°N y 40°S. Se utilizaron descriptores como: “deficiency”, “vitamin D”, “pregnancy”, “causes”, “perinatal outcomes” y “supplementation”, y sus respectivos en español para bases de datos validadas. Se tabularon matrices según PRISMA modificada.
Se obtuvieron 8 estudios en inglés pertenecientes solo al hemisferio norte, la mayoría recomendables desde la calidad de evidencia y que por ronda de expertas se relacionaron con el rol de la matronería. Los resultados arrojaron causas como: origen de la gestante, etnia, baja exposición solar, obesidad, nivel socioeconómico; riesgos perinatales: preclampsia, diabetes gestacional y parto prematuro; así como también diversas dosis de suplementación que van de los 200 a 4400 UI/día. No se encontraron estudios en gestantes del hemisferio sur o en referidos rol de matronería en dicho ámbito.
Como conclusión el déficit de vitamina D es un problema de salud pública, se deben considerar los determinantes sociales y el perfil epidemiológico de las gestantes, especialmente de zonas extremas, por su latitud, y para ello se sugieren investigaciones experimentales e incorporación de fortificación y suplementación como política pública.
KECERDASAN EMOSI DAN PERILAKU PROSOSIAL PADA PERAWAT
Authors: Aprilia Eka Dian , Mauritsatina Mauritsatina 
Published: November 2022

Saat menjalankan tugasnya, perawat dituntut harus menumbuhkan rasa saling menolong, mampu bekerja sama dan bersikap ramah yang merupakan wujud dari perilaku prososial. Salah satu faktor yang mempengaruhi perilaku prososial adalah kecerdasan emosi. Semakin tinggi kecerdasan emosi maka akan semakin tinggi perilaku prososial. Penelitian ini bertujuan untuk mengetahui apakah terdapat hubungan antara kecerdasan emosi dengan perilaku prososial pada perawat di Rumah Sakit X. Sampel dalam penelitian ini sebanyak 161 orang dengan menggunakan teknik pengambilan data incidental sampling. Penelitian ini menggunakan Skala Kecerdasan Emosi yang disusun oleh Mawarpury (2004) berdasarkan aspek yang disampaikan oleh Goleman (2000) yang kemudian dimodifikasi, skala ini terdiri atas 27 aitem pernyataan dan Skala Perilaku Prososial yang disusun oleh peneliti berdasarkan pada teori Eisenberg dan Mussen (1989), skala ini terdiri atas 27 aitem pernyataan. Hasil analisis data menggunakan teknik analisis pearson product-moment menunjukkan koefisien korelasi (r) sebesar 0,789 dengan nilai p = 0,000 (p < 0.05). Hasil analisa tersebut menunjukkan bahwa terdapat hubungan positif dan signifikan antara kecerdasan emosi dengan perilaku prososial pada perawat di Rumah Sakit X. Hasil analisa juga menunjukkan semakin tinggi kecerdasan emosi maka semakin tinggi perilaku prososial pada perawat di Rumah Sakit X.