bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparaison du pouvoir pathogène de trois souches de <em>Mycoplasma capricolum</em> pour la chèvre et le chevreau nouveau-né
Authors: Taoudi A. , Karib H. , Johnson D.W. , Fassi Fehri M.M. 
Published: August 2022

Deux essais d’infection expérimentale de caprins par Mycoplasma (M.) capricolum ont été conduits. Des caprins adultes, infectés par voie intrabronchique à la dose de 109 CFU/ml, à l’aide d’une souche d’origine ovine (O12), ont montré des troubles respiratoires discrets et transitoires ; ils ont excrété le germe pendant les 5 semaines qu’a duré l’observation et ont développé une séroconversion, décelée dès la première semaine par un test ELISA. Des chèvres, allaitant leurs produits au premier mois, ont été réparties en 3 lots et inoculées à la dose de 2.107 CFU/ml dans le trayon gauche, le premier lot à l’aide de la souche O12, le second à l’aide d’une souche marocaine caprine et le dernier par une souche australienne caprine. Une mammite sévère a été observée dans tous les lots, avec répercussion sur l’état général sauf dans le lot infecté par la souche O12. L’infection s’est transmise par la tétée aux nouveau-nés dont elle a entraîné la mort en 6 à 12 jours sauf dans le cas d’un chevreau âgé d’un mois au moment de son infection par la souche O12. Ces résultats suggèrent que :
- les caprins adultes opposent une assez bonne résistance à l’infection par M. capricolum, par voie intrabronchique ;
- il y aurait une variabilité dans la virulence des souches de M. capricolum, la souche O12 étant moins virulente, aussi bien pour les chèvres que pour les chevreaux.
Catanionic Surfactants for Improving Oil Production in Carbonate Reservoirs
Authors: Xu Limin , Han Ming , Cao Dongqing , Fuseni Alhasan 
Published: August 2022

Summary
This paper presents the development of catanionic surfactants composed of cationic and anionic surfactants to make them high-performance products for chemical flooding in high-temperature and high-salinity carbonate reservoirs. The objective of this study is to optimize the surfactant chemistry by mixing oppositely charged anionic surfactants and cationic surfactants (CASs), which results in significant synergistic effects in interfacial properties due to electrostatic attraction to improve oil production at the given harsh conditions. The optimal mixing surfactant ratios were determined according to the brine-surfactant compatibility, microemulsion phase behavior, and the interfacial tension (IFT) between oil and surfactant solutions in high-salinity brine at 90°C. Comprehensive performance of the catanionic surfactants was evaluated, including adsorption of the surfactants onto the carbonate rocks and the long-term stability at 95°C. The coreflooding displacement experiments were performed using carbonate core plugs at 95°C to evaluate the potential of the optimal catanionic surfactant in improving oil recovery. Three catanionic surfactants with good compatibility were developed in this study. It appeared that the synergistic effect between the mixing surfactants was enhanced with increasing temperature. Although the IFT of the individual surfactants with crude oil was between 10−1 and 100 mN/m, a significant IFT reduction in the magnitude of 10−2 to 10−3 mN/m was observed by mixing the selected anionic surfactants and CASs. A salinity scan showed that the IFT values maintained a value of 10−2 mN/m in a wide salinity range, which demonstrated the effectiveness of the catanionic surfactant. In microemulsion phase behavior studies, the developed catanionic surfactant solution in the presence of crude oil exhibited Winsor Type III emulsions. The static adsorption quantities of the catanionic surfactants were lower than the values of the individual surfactants. All these indicated the feasibility of catanionic surfactants for their applications in the harsh reservoir conditions. The results of coreflooding displacement tests demonstrated significant oil recovery improvement beyond waterflooding. This work provides an efficient way to get surfactant formulations by mixing oppositely charged surfactants to obtain high performance in improving oil production under harsh conditions.
Cognitive Flexibility and Emotion Regulation as Transdiagnostic Mechanisms of Psychopathology in Clinically-Referred Youths
Authors: Coenye Jakke , Verbeken Sandra , Braet Jolien , Braet Caroline , Moens Ellen , Goossens Lien 
Published: August 2022

Abstract
An increasing amount of reports sound the alarm about our youngsters mental health state. Research on transdiagnostic mechanisms is needed. Recently, attention has been drawn to the role of cognitive flexibility (CF; the ability to adapt thoughts and behavior to contextual changes), as a possible risk factor for psychopathological development, yet research among children and adolescents is scarce. We hypothesize both direct and indirect links whereby (mal)adaptive emotion regulation strategies might mediate this relationship. Results of this study with 192 clinically-referred children and adolescents (65% female; mean age = 12.65; SD = 2.99) indicated that CF was related to both internalizing and externalizing symptoms. However, this relationship was not mediated by adaptive or maladaptive emotion regulation strategies. Implications for theory, clinical practice and future research are discussed.
FloraBERT: cross-species transfer learning withattention-based neural networks for geneexpression prediction
Authors: Levy Benjamin , Xu Zihao , Zhao Liyang , Kremling Karl , Altman Ross , Wong Phoebe , Tanner Chris 
Published: August 2022

Abstract
Recent work in applying deep learning models has demonstrated that endophenotypes, such as RNA transcript abundance, can be predicted from an organism's regulatory DNA. However, due to the vast amount of labelled data required to train previous types of deep learning models, this work has been constrained to species with large amounts of data labelled for a particular task. Here, we present FloraBERT, a transfer-learning based deep learning model that is able to improve predictions of gene expression in a single target species, and it does so by exploiting cross-species genomic information in the form of genome assemblies from all of plantae. FloraBERT significantly outperforms simple bag-of-k-mers baseline models and achieves comparable performance to prior work that concerns less complex species. Furthermore, investigation of the learned parameters of FloraBERT reveals that the training process encodes biologically salient information, such as taxonomic similarity between species and positional relevance of nucleotides within a promoter. To facilitate future research, we have made the source code and model weights publicly available on https://github.com/benlevyx/florabert.
Vam6 upregulated by lactic acid inhibits anti-tumor effects of intratumoral iNKT cells via modulating AMPK/mTOR pathways
Authors: Zhang Huimin , Bai Shiyu , Wu Qielan , Zhu Shasha , Zhang Yuwei , Chen Xuran , Pan Jun , Li Shuhang , Yue Ting , Xu Linfeng , Xie Di , Tian Chenxi , Li Xiang , Hou Junjie , Wang Lu , Fu Sicheng , Xue Yanhong , Jiang Amin , Li Dong , Xu Tao , Tian Zhigang , Zhou Rongbin , Bai Li 
Published: August 2022

Abstract
Activation of mTORC1 is essential for anti-tumor function of iNKT cells. The mechanisms underlying impaired mTORC1 activation in intratumoral iNKT cells remain unclear. Here, we showed that lactic acid from tumor cells increased Vam6 expression in iNKT cells which led to impaired mTORC1 activation and IFN-γ production. Mechanistically, Vam6 in iNKT cells was essential for Rab7a-Vam6-AMPK complex formation and thus for recruitment of AMPK to lysosome to activate AMPK, a negative regulator of mTORC1. Additionally, Vam6 relieved inhibitory effect of VDAC1 on Rab7a-Vam6-AMPK complex formation at mitochondria-lysosome contact site. Given the key roles of lactic acid-increased Vam6 in promoting AMPK activation in intratumoral iNKT cells, reducing Vam6 expression significantly enhanced the mTORC1 activation in intratumoral iNKT cells as well as their anti-tumor efficacy. Together, we propose Vam6 as a target for iNKT cell-based immunotherapy.
Genetic Etiology in Patients Diagnosed With Congenital Hypothroidism With New Generation Sequencing: a Single Center Experience
Authors: kaplan emel hatun aytaç , mermer serdar 
Published: August 2022

Abstract
Aim: Congenital hypothyroidism (CH) is the most common endocrine disorder of the newborn; however, it is seen in every 3000-4000 births (1). The identification of genetic mutations is vital for the continuation of treatment, especially in patients with unidentified dysgenesis and etiology. The present study aims to contribute to the literature sharing the patients of CH with mutations and show the benefit it will provide to patients in follow-up.Method: A total of 52 patients (47 families) diagnosed with CH were examined in the study. Age at diagnosis, TSH and fT4 levels at diagnosis, and thyroid volumes based on age were evaluated. 32 target gens (GLIS3, TSHB, THRA, PAX8, NKX2-5, NKX2-1, FOXE1, TSHR, SLC5A5, SLC26A4, TG, TPO, DUOX2, DUOXA2, IYD, SLC26A7, DUOX1, ZNF607, SLC6A4, DIO1, DIO2, DIO3, TTR, GNAS, TRH, ALB, POR, TRHR, PHEX, SLC16A2, SERPINA7, IGSF1) identified in the etiology of CD by next-generation sequencing (NGS) were investigated. The pathogenicity of the variants was evaluated using the American College of Medical Genetics (ACGM) guidelines, inheritance pattern and published evidence.Results: 29 (55%) of the patients were male. The rate of dysgenesis was 19.2%. Genetic studies were performed in all patients, regardless of transient-permanent hypothyroidism. The identification frequency of mutation was 55% (n=29, 33 different variants). The members of the same family had similar variants. There were 2 patients with a similar variant without being from the same family (patient 13,14,15:TPO K47R and patient 17,18:NKX2-1 A93P variant). There were 21 patients (40.3%) in whom dyshormonogenesis was shown. The most common mutation was DUOX2 (20%). The second most common mutations were mutations in TPO and TG genes (15%, 15%). The ACGM classification was made. 41.1% of these mutations were VUS, 26.4% were pathogenic, 23.5% were likely benign, and 11.7% were likely pathogenic. 73.5% heterozygous, 17.6% homozygous, and 8.9% combined heterozygous mutations were identified based on their zygosities. There were mutant variants in two different genes in siz patients and in three different genes in one patient. One of the two siblings (patient 23,24) with SLC5A5 mutation had goiter and one had hypoplasic thyroid tissue. Three frameshift mutations were detected.Conclusion: This method shed light on the etiology of 55% of the study patients with CH, and it was effective in the treatment decision of the two patients. The frequency of VUS was high. The presence of multiple genes and multiple variants in the same patient is a promising result of the study. Moreover, the present study reveals the gene profile of patients with CH in Turkey.
A market sharing mechanism for watershed ecological compensation
Authors: Deng Menghua , Chen Junfei 
Published: August 2022

Abstract
This study establishes a market sharing mechanism for watershed ecological compensation between an upstream and downstream government based on the game-theoretical approach. It is found that, in this transboundary market sharing mechanism, the upstream government is encouraged to make more effort to protect the watershed ecosystem. As a result, a market-sharing compensation mechanism can bring Pareto improvements to all stakeholders. Furthermore, the influences of several key factors on the performance of the market sharing mechanism are investigated, which provides theoretical guidance for establishing and implementing such a mechanism in practice.
The role of attached bacteria in the formation ofMicrocystiscolony in Chentaizi River
Authors: Dai Wei , Ruan Weilin , Bi Xiangdong , Zhang Dajuan 
Published: August 2022

AbstractTo further understand the role of attached bacteria in the formation of Microcystis colonies, we conducted a field investigation in Chentaizi River in Tianjin, China, which frequently suffers Microcystis blooms in summer. The results showed the average cell density of Microcystis was 2.31 × 107 cell/L from July 19 to July 27, 2021. Free-living and attached bacteria communities shared similar phylum diversity, but the abundance changed obviously. The colony size of Microcystis and attached bacterial number in the colony showed an increasing trend during the whole sampling period. There was a significant positive correlation between Microcystis colonial size and attached bacterial density (P &lt; 0.01), indicating attached bacteria could contribute the colony formation of Microcystis. The genus composition in attached bacterial community varied in colonies of different sizes. The relative abundance of Acinetobacter, Cloacibacterium, Sphingobacterium, and Ralstonia in &gt;90 μm colonies were significantly higher than those in 8–20 μm and 20–90 μm colonies (P &lt; 0.05). These genera might have positive effects on the colony formation of Microcystis during sampling.
Cloning, Expression, and Functional Analysis of the Full-Length cDNA of
Acetyl-CoA C-acetyltransferase (AACT) Genes Related to Terpenoid
Synthesis in Platycodon grandiflorus
Authors: Liu Mengli , Yu Hanwen , Li Jing , Dong Nan , Chen Bowen , Xu Rui , Wu Junxian , Chang Xiangwei , Wang Jutao , Peng Huasheng , Zha Liangping , Gui Shuangying 
Published: August 2022

Abstract:
Platycodon grandiflorus is a well-known and widely distributed traditional herbal
medicine and functional food in Asia, with triterpenoids as the main bioactive component in its roots.
Acetyl-CoA C-acetyltransferase (AACT) is the initiation enzyme in the mevalonate pathway and
plays an important role in the biosynthesis of terpenoids.

Objective:
The objective of this study was to clone and identify the PgAACT function in P.
grandiflorus.

Method:
The full-length sequence of PgAACT genes was isolated and cloned from P. grandiflorus
by polymerase chain reaction (PCR). The recombinant plasmid was constructed using the pET-32a
vector and expressed in E. coli Transetta (DE3) cells. Subcellular localization of AACT was
observed in the epidermal cells of N. tabacum. Quantitative reverse transcription-PCR (qRT-PCR)
was used to identify the PgAACT gene transcription levels. After MeJA treatment, the changes in
AACT gene expression were observed, and UHPLC-Q-Exactive Orbitrap MS/MS was used to detect
the changes in P. grandiflorus saponins.

Results:
In this study, two full-length cDNAs encoding AACT1 (PgAACT1) and AACT2
(PgAACT2) were isolated and cloned from P. grandiflorus. The deduced PgAACT1 and PgAACT2
proteins contain 408 and 416 amino acids, respectively. The recombinant vectors were constructed,
and the protein expression was improved by optimizing the reaction conditions. Sodium dodecyl
sulphate-polycrylamide gel electrophloresis and western blot analysis showed that the PgAACT
genes were successfully expressed, with molecular weights of the recombinant proteins of 61 and 63
kDa, respectively. Subcellular localization showed that the PgAACT genes were localized in the
cytoplasm. Tissue specificity analysis of P. grandiflorus from different habitats showed that
PgAACT genes were expressed in the roots, stems, and leaves. After MeJA treatment, the expression
level of PgAACT genes and the content of total saponins of P. grandiflorus were significantly
increased, suggesting that PgAACT genes play an important role in regulating plant defense systems.

Conclusion:
Cloning, expression, and functional analysis of PgAACT1 and PgAACT2 will be helpful
in understanding the role of these two genes in terpene biosynthesis.
Synthesis, Molecular Docking, c-Met Inhibitions of 2,2,2-Trichloroethylidene- cyclohexane-1, 3-dione Derivatives Together with their Application as Target SARS-CoV-2 main Protease (Mpro) and as Potential anti-COVID-19
Authors: Almutairi Fahad M. , Mohareb Rafat M. , Elfiky Abdo A. , Abdelaziz Mahmoud A. , Wardakhan Wagnat W. , Mohamed Mervat S. , Abdelhameed Ali S. 
Published: August 2022

Background:The lack of anti-COVID-19 treatment to date warrants urgent research into potential therapeutic targets. Virtual drug screening techniques enable the identification of novel compounds that target the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Main Protease (Mpro).Objective:The binding of the halogenated compounds to Mpro may inhibit the replication and transcription of SARS-CoV-2 and, ultimately, stop the viral life cycle. In times of dire need for anti- COVID-19 treatment, this study lays the groundwork for further experimental research to investigate these compounds' efficacy and potential medical uses to treat COVID-19.Method:New heterocyclic compounds were synthesized through the first reaction of cyclohexane- 1, 3-dione (1a) or dimedone (1b) with trichloroacetonitrile (2) to give the 2,2,2-trichloroethylidene) cyclohexane-1,3-dione derivatives 3a and 3b, respectively. The latter compounds underwent a series of heterocyclization reactions to produce biologically active compounds.Results:Novel compounds, including fused thiophene, pyrimidine and pyran derivatives, were synthesized and tested against human RNA N7-MTase (hRNMT) and selected viral N7-MTases such as SARS-CoV nsp14 and Vaccinia D1-D12 complex to evaluate their specificity and their molecular modeling was also studied in the aim of producing anti-covid-19 target molecules.Conclusion:The results showed that compounds 10a, 10b, 10c, 10e, 10f, 10g and 10h showed high % inhibitions against SARs-Covnsp 14. Whereas compounds 5a, 7a, 8b, 10a, 10b, 10c and 10i showed high inhibitions against hRNMT. This study explored the binding affinity of twenty-two halogenated compounds to the SARS-CoV-2 MPro and discovered fifteen compounds with higher binding affinity than Nelfinavir, of which three showed remarkable results. c-Met kinase inhibitions of 10a, 10f, 10g and 10h showed that all compounds exhibited higher inhibitions than the reference Foretinib.
The Roles and Regulation of Ferroptosis in Cancer Progression
Authors: yang Yu , Ma Te , Hu Ting , Ma Chengcheng , Tu Huiqing , Zhu Shunqin 
Published: August 2022

Abstract:
Ferroptosis is an iron-dependent, nonapoptotic form of regulatory death and has received
extensive attention. Fenton reaction related to iron metabolism release high levels of Reactive Oxygen
Species (ROS), and the intracellular ROS content is closely related to various diseases; the iron
ion concentration in many diseased cells is also disordered. In this paper, the advances in ferroptosis
research are summarized, and the regulatory mechanisms of ferroptosis, including inducers and regulatory
protein of ferroptosis in cancer progression. We expect that this study will benefit the further
development of basic research and clinical application of ferroptosis for cancer treatment.
Interaction of Nanomaterials with Protein-Peptide
Authors: Jha Anupam Nath , Hazarika Zaved , Saikia Surovi 
Published: August 2022

Abstract:
Nanomaterials have undergone rapid development in the last few decades, galvanized by the versatility of their functional attributes and many inherent advantages over bulk materials. The state of art experimental techniques to synthesize nanoparticles (NPs) from varied sources, offers unprecedented opportunities for utilization and exploration of multifaceted biological activities. Such formulations demand preliminary understanding of the interaction between NPs and biomolecules. Most of these interactions are dependent on the external morphology of the NPs, like the shape, size, charge and surface chemistry. In addition, majority of the experimental techniques are limited to cellular level data, without the atomistic details of mechanisms that lead to these interactions. Consequently, recent studies have determined these atomistic events through in-silico techniques, which provides better understanding and integrative details of interactions between biomolecules and different NPs. Therefore, while delineating the protein-NPs interaction, it is imperative to define the consequences of nanomaterial’s introduction and derive data for formulation of better therapeutic interventions. This review briefly discusses varied types of NPs, their potential applications and interactions with peptide and proteins.
Efficacy and Safety of Alteplase on Treatment of Acute Single Small
Subcortical Infarction
Authors: Gao Yuan , Xu Yuming , Marcelinus Kilanga , Liu Hongbing , Zhang Ke , Zong Ce , Yang Hongxun , Song Bo 
Published: August 2022

Background:
Single small subcortical infarction (SSSI) is an isolated small infarction in the territory of perforating artery with a maximum diameter of 20 mm in axial diffusion-weighted imaging (DWI). About 20 to 30% of SSSI patients were reported to have early neurological deterioration (END) in the acute phase which brought adverse effects on long-term outcome. The effect of the alteplase on the outcome of SSSI, especially END and long-term outcome was ambiguous.

Objective:
To find out the efficacy and safety of intravenous recombinant tissue Plasminogen Activator(rt-PA) on long-term and short- outcomes of patients with SSSI as compared to patients who received standard medical care.

Method:
The patients were retrospectively screened from a stroke registry of the neurology department of 1st Affiliated Hospital of Zhengzhou University from January 2013 to December 2020.Based on treatment modality patients were dichotomized into alteplase and standard medical care group. To minimize confounding factors in subgroups, a propensity score matching analysis was done. The primary outcome was favorable functional outcome on 3 months after stroke onset defined by attaining score of ≤2 points on the modified Rankin scale (mRS), secondary outcome was prevention of occurrence of END defined as an increase of ≥2 points in total score or ≥1point on motor subunit in the National Institutes of Health Stroke Scale (NIHSS) score within 72 hour of symptoms onset, safety features were symptomatic intracranial hemorrhage (sICH) or death. Multivariate analysis was employed to find the efficacy and safety of alteplase on the treatment of SSSI.

Results:
A total of 717 patients with anterior circulation SSSI were selected, 132 were included in the final analysis. Forty-five patients were treated with alteplase within 4.5 hours and 87 with standard medical care and 44 pairs were successfully matched by propensity score. Pre-match data showed that alteplase thrombolysis group showed higher proportion of favorable outcomes at 3-month follow-up [ OR=0.315, 95%CI:0.106, 0.931, P=0.037], but did not reduce the incidence of END compared with the non-thrombolytic group [ OR=1.033, 95%CI :0.417,2.554, P=0.943]. Post-match data showed that the alteplase group also showed higher proportion of favorable outcomes at 3-month follow-up [ OR=0.247, 95%CI: 0.074, 0.830, P=0.024], however, it did not reduce the incidence of END compared with the non-thrombolytic group [ OR=1.241, 95%CI: 0.433,3.554, P=0.688]. There was one case of asymptomatic ICH in alteplase treated patients.

Conclusion:
Patients with SSSI in the anterior circulation are more likely to achieve 3 months favorable outcomes than those who were treated with standard medical care, however treatment with alteplase may not prevent occurrence of END.