bluuebunny/crossref_metadata_2025_split huggingface dataset:
The science of child and adolescent mental health in Greece: a nationwide systematic review of the literature
Authors: Koumoula Anastasia , Marchionatti Lauro Estivalete , Caye Arthur , Karagiorga Vasiliki Eirini , Balikou Panagiota , Lontou Katerina , Arkoulaki Vicky , Simioni André , Serdari Aspasia , Kotsis Konstantinos , Basta Maria , Kapsimali Efi , Mitropoulou Andromachi , Klavdianou Nikanthi , Zeleni Domna , Mitroulaki Sotiria , Botzaki Anna , Gerostergios Giorgos , Samiotakis Giorgos , Moschos Giorgos , Giannopoulou Ioanna , Papanikolaou Katerina , Aggeli Katerina , Scarmeas Nikolaos , Koulouvaris Panagiotis , Emanuele Jill , Schuster Kenneth , Karyotaki Eirini , Kalikow Lily , Pronoiti Katerina , Gosmann Natan Pereira , Schafer Julia Luiza , Merikangas Kathleen R. , Szatmari Peter , Cuijpers Pim , Georgiades Katholiki , Milham Michael P. , Corcoran Mimi , Burke Sarah , Koplewicz Harold , Salum Giovanni Abrahão 
Published: October 2022

AbstractBackgroundEvidence-based information is essential to delivering effective mental health care, yet the extent and accessibility of the scientific literature are critical barriers for professionals and policymakers. To map the necessities and make validated resources accessible, we undertook a comprehensive analysis of scientific evidence on child and adolescent mental health in Greece.MethodsThis systematic review encompasses three research topics related to the mental health of children and adolescents in Greece: prevalence estimates, assessment instruments, and interventions. We searched Pubmed, Web of Science, PsycINFO, Google Scholar, and IATPOTEK from inception to December 16th, 2021. We included studies assessing the prevalence of conditions, reporting data on assessment tools, and experimental interventions. For each area, manuals informed data extraction and the methodological quality was ascertained using validated tools. This review was registered in protocols.io [68583].OutcomesWe included 104 studies reporting 533 prevalence estimates, 223 studies informing data on 261 assessment instruments, and 34 intervention studies. We report the prevalence of conditions according to regions within the country. A repository of locally validated instruments and their psychometrics were compiled. An overview of interventions provided data on their effectiveness. The outcomes are made available in an interactive resource on-line [https://camhi.gr/en/systematic-review-tables/].InterpretationScientific evidence on child and adolescent mental health in Greece has been cataloged and appraised. This timely and accessible compendium of up-to-date evidence offers valuable resources for clinical practice and policy making in Greece and may encourage similar assessments in other countries.FundingThe Stavros Niarchos Foundation.
Metastability as a neuromechanistic biomarker of schizophrenia pathology
Authors: Hancock Fran , Rosas Fernando E. , McCutcheon Robert A. , Cabral Joana , Dipasquale Ottavia , Turkheimer Federico E. 
Published: October 2022

AbstractThe disconnection hypothesis of schizophrenia proposes that symptoms of the disorder arise as a result of aberrant functional integration between segregated areas of the brain. The concept of metastability characterizes the coexistence of competing tendencies for functional integration and functional segregation in the brain and is therefore well suited for the study of schizophrenia. In this study we investigate metastability as a neuromechanistic biomarker of schizophrenia pathology, including a demonstration of reliability and face validity.Group-level discrimination, individual-level classification, and pathophysiological relevance were assessed using two independent case-control studies of schizophrenia, the Human Connectome Project Early Psychosis (HCPEP) study (controlsn=82, non-affective psychosisn=53) and the Cobre study (controlsn=71, casesn=59). In this work we introduce a new framework that uses Leading Eigenvector Dynamic Analysis (LEiDA) to capture specific features of dynamic functional connectivity and then implements a novel approach to estimate metastability. We used non-parametric testing to evaluate group-level differences and a naïve Bayes classifier to discriminate cases from controls.Our results show that our new approach is capable of discriminating cases from controls with elevated effect sizes relative to published literature, reflected in an up to 76% area under the curve (AUC) in out-of-sample classification analyses. Furthermore, our analyses demonstrated that patients with early psychosis exhibit intermittent disconnectivity of subcortical regions with frontal cortex and cerebellar regions, introducing new insights about the mechanistic bases of these conditions.Overall, these findings demonstrate reliability and face validity of metastability as a neuromechanistic biomarker of schizophrenia pathology.
Diabetes and Cognitive Health in India: A Nationally Representative Survey of Adults Aged 45 Years and Older
Authors: Flood David , Green Hunter , Gross Alden L. , Kobayashi Lindsay C. , Levine Deborah A. , Lee Jinkook , Wixom Caroline R. , Langa Kenneth M. 
Published: October 2022

ABSTRACTBackgroundFew studies have assessed the relationship between diabetes and cognitive health outside of high-income countries. We described this relationship in India, a middle-income country of 1.4 billion people.MethodsData were analyzed from 58,027 adults aged ≥45 years in the baseline wave of the nationally representative Longitudinal Aging Study in India, from 2017 to 2019. Outcomes were scaled cognitive scores (mean of 0 and standard deviation [SD] of 1) and cognitive impairment, defined as a cognitive score 1.5 standard deviations or below the age- and education-matched mean. Diabetes was defined as a self-report of a prior diabetes diagnosis made by a health professional or having a measured hemoglobin A1c ≥6.5% at the time of the LASI survey. To describe the cross-sectional relationship between cognitive health and diabetes, we constructed survey-weighted multivariable linear and logistic regression models controlling for prespecified covariates, including age, sex, sociodemographic characteristics, and rural versus urban residence.ResultsIn age- and sex-adjusted models, people with diabetes had cognitive scores that were 0.25 SD higher (95% CI: 0.23 to 0.27) and had a 1.3% (95% CI: 0.7 to 1.8) lower prevalence of cognitive impairment than people without diabetes. Differences persisted even when adjusting for demographic, socioeconomic, and geographic characteristics. Rural versus urban residence modified the relationships of diabetes with cognitive score (P = 0.001) and cognitive impairment (P = 0.004). In fully adjusted models, rural respondents with diabetes had 0.05 SD (95% CI: 0.03 to 0.07) greater cognitive scores and 1.7% (95% CI: 0.9 to 2.5) lower prevalence of cognitive impairment than those without diabetes. In urban areas, respondents with and without diabetes had similar cognitive scores and prevalence of cognitive impairment.ConclusionUrban-rural health differences, the nutrition transition, and social conditions likely influence the observed cross-sectional relationship between diabetes and cognitive dysfunction in India, leading to different associations than previously reported in many high-income countries.FundingNational Institutes of Health, USA and the Ministry and of Health and Family Welfare, Government of India
Multi-omic profiling of the developing human cerebral cortex at the single cell level
Authors: Zhu Kaiyi , Bendl Jaroslav , Rahman Samir , Vicari James M. , Coleman Claire , Clarence Tereza , Latouche Ovaun , Tsankova Nadejda M. , Li Aiqun , Brennand Kristen J. , Lee Donghoon , Yuan Guo-Cheng , Fullard John F. , Roussos Panos 
Published: October 2022

AbstractThe cellular complexity of the human brain is established via dynamic changes in gene expression throughout development that is mediated, in part, by the spatiotemporal activity of cis-regulatory elements. We simultaneously profiled gene expression and chromatin accessibility in 45,549 cortical nuclei across 6 broad developmental time-points from fetus to adult. We identified cell-type specific domains in which chromatin accessibility is highly correlated with gene expression. Differentiation pseudotime trajectory analysis indicates that chromatin accessibility at cis-regulatory elements precedes transcription and that dynamic changes in chromatin structure play a critical role in neuronal lineage commitment. In addition, we mapped cell-type and temporally specific genetic loci implicated in neuropsychiatric traits, including schizophrenia and bipolar disorder. Together, our results describe the complex regulation of cell composition at critical stages in lineage determination, serve as a developmental blueprint of the human brain and shed light on the impact of spatiotemporal alterations in gene expression on neuropsychiatric disease.One-Sentence SummarySimultaneous profiling of gene expression and chromatin accessibility in single nuclei from 6 developmental time-points sheds light on cell fate determination in the human cerebral cortex and on the molecular basis of neuropsychiatric disease.
Cortical gradients during naturalistic processing are hierarchical and modality-specific
Authors: Samara Ahmad , Eilbott Jeffrey , Margulies Daniel S. , Xu Ting , Vanderwal Tamara 
Published: October 2022

AbstractUnderstanding cortical topographic organization and how it supports complex perceptual and cognitive processes is a fundamental question in neuroscience. Previous work has characterized functional gradients that demonstrate large-scale principles of cortical organization. How these gradients are modulated by rich ecological stimuli remains unknown. Here, we utilize naturalistic stimuli via movie-fMRI to assess macroscale functional organization. We identify principal movie gradients that delineate separate hierarchies anchored in sensorimotor, visual, and auditory/language areas. At the opposite/heteromodal end of these perception-to-cognition axes, we find a more central role for the frontoparietal network along with the default network. Even across different movie stimuli, movie gradients demonstrated good reliability, suggesting that these hierarchies reflect a brain state common across different naturalistic conditions. The relative position of brain areas within movie gradients showed stronger and more numerous correlations with cognitive behavioral scores compared to resting state gradients. Together, these findings provide an ecologically valid representation of the principles underlying cortical organization while the brain is active and engaged in multimodal, dynamic perceptual and cognitive processing.HighlightsMovie-fMRI reveals novel, more granular principles of hierarchical cortical organizationTop movie gradients delineate three separate perception-to-cognition hierarchiesA distinctive third gradient in movie-watching is anchored by auditory/language regionsGradient scores demonstrate good reliability even across different movie stimuliMovie gradients yield stronger correlations with behavior relative to resting state gradients
Decolonizing WASH Research: Results of a qualitative study and consensus-building process to develop principles for increasing equity in WASH Research
Authors: Lue J’Anna-Mare , Bah Salamata , Grant Kaelah , Lee Justine , Nzekele Leila , Tidwell James B 
Published: October 2022

AbstractThere have long been critiques of colonial legacies influencing global health. In recent years with growing public awareness of unjust systems, a new wave of calls for anti-racist initiatives and decolonization of the sector has emerged. This study examined research inequities in the water, sanitation, and hygiene (WASH) sector, centering the perspectives of researchers from low- and middle-income countries (LMICs), to catalyze collective action in a sub-sector of global health.Nineteen semi-structured interviews were conducted with researchers of different backgrounds regarding nationality, gender, and research experience. Researchers from eight countries were asked about their experiences and direct observations of discrimination across various stages of the research process. Five interviews were conducted with key WASH research funders to assess perceptions of obstacles faced by LMIC researchers, successes achieved, and challenges faced by these organizations when working towards more equitable research processes within the WASH sector.The results were analyzed using an emergent framework that categorized experiences based on power differentials and abuse of power; structural barriers due to organizational policies; institutional and individual indifference; othering speech, action, and practices; and context-specific discrimination. The Socio-Ecological Model was also combined with this framework to identify the types of actors and level of coordination needed to address these issues. Respondents were often reluctant to describe actions as discriminatory unless there was clear intent. Researchers who worked in both LMICs and HICs at different career stages were particularly aware of discrimination.Ensuring pro-equity authorship and funding practices were identified as two significant actions to catalyze change within the sector. Sector-wide efforts must center LMIC voices when identifying research questions, conducting research, and dissemination. Individuals, organizations, and the entire WASH sector must examine how they participate in upholding inequitable systems of power to begin to dismantle the system through the intentional yielding of power and resources.
The death toll of armed conflict and food insecurity in north-east Nigeria, 2016-2019: a statistical study
Authors: Checchi Francesco , Gimma Amy , Jarvis Christopher I , van Zandvoort Kevin , Warsame Abdihamid 
Published: October 2022

AbstractBackgroundArmed conflict, compounded by displacement and food insecurity, has affected Adamawa, Borno and Yobe states of northeast Nigeria (population ≈ 12 million) since 2009. The crisis escalated in 2013-2014 and featured a delayed humanitarian response. We wished to estimate the death rate and toll attributable to the crisis. Data availability constraints restricted estimation to the period 2016 to 2019.MethodsWe did a small-area estimation statistical analysis of previously collected data, stratified by local government area and month. We fitted a mixed effects model to household mortality data collected as part of 70 ground surveys implemented by humanitarian actors within the study area and period. Model predictors, selected from a wider candidate list, included livelihood typology, the price of staple cereal, vaccination geo-coverage and the presence of humanitarian actors. To project accurate death tolls, we adjusted population denominators based on internal and refugee displacements. We used the model and population estimates to project mortality under observed conditions and under different assumptions of counterfactual conditions, had there been no crisis, with the difference between observed and counterfactual providing excess mortality.ResultsCrude and under 5 years death rates were highly elevated across most ground surveys, with net negative household migration. Between April 2016 and December 2019, we projected that 490,000 deaths (230,000 children under 5y) died in excess of the counterfactual non-crisis level; this death toll ranged from medians of 90,000 to 550,000 depending on the counterfactual assumptions chosen, specifically whether to consider a Nigeria-wide price inflation phase as inherently part of the crisis in the north-east. Crude and under 5 years death rates were two to three times higher than counterfactual levels, and highest in 2016-2017.DiscussionThis may be the first crisis-wide estimate of mortality attributable to the crisis in north-east Nigeria. Our findings do not reflect acute emergency periods before 2016 and the situation in neighbouring countries affected by the conflict. Sensitivity analysis suggests that results for Borno state, where conflict has been most intense, are subject to under-estimation due to data not representing hard-to-access areas. Further studies to document mortality in this and other crises are needed to guide decision-making and memorialise their human toll.
Improvedin situsequencing for high-resolution targeted spatial transcriptomic analysis in tissue sections
Authors: Tang Xinbin , Chen Jiayu , Zhang Xinya , Liu Xuzhu , Xie Zhaoxiang , Wei Kaipeng , Qiu Jianlong , Ma Weiyan , Lin Chen , Ke Rongqin 
Published: October 2022

ABSTRACTSpatial transcriptomics enables the study of localization-indexed gene expression activity in tissues, providing the transcriptional landscape that in turn indicates the potential regulatory networks of gene expression.In situsequencing (ISS) is a targeted spatial transcriptomic technique, which is based on padlock probe and rolling circle amplification combined with next-generation sequencing chemistry, for highly multiplexedin situgene expression profiling. Here, we present improvedin situsequencing (IISS) that exploits a new probing and barcoding approach, combined with advanced image analysis pipeline for high-resolution targeted spatial gene expression profiling. We developed an improved combinatorial probe anchor ligation chemistry using a 2-base encoding strategy for barcode interrogation. The new encoding strategy resulted in higher signal intensity as well as improved specificity forin situsequencing, while maintaining a streamlined analysis pipeline for targeted spatial transcriptomics. We showed that IISS can be applied to both fresh frozen tissue and formalin-fixed paraffin embedded tissue sections for single cell level spatial gene expression analysis, based on which the developmental trajectory and cell-cell communication networks can also be constructed. In conclusion, our method is a versatile molecular tool for targeted spatial transcriptomic analysis.
Legionellapara-effectors target chromatin and promote bacterial replication
Authors: Schator Daniel , Mondino Sonia , Berthelet Jérémy , Di Silvestre Cristina , Ben Assaya Mathilde , Rusniok Christophe , Rodrigues-Lima Fernando , Wehenkel Annemarie , Buchrieser Carmen , Rolando Monica 
Published: October 2022

ABSTRACTLegionella pneumophilareplicates intracellularly by secreting effectorsviaa type IV secretion system. One of these effectors is a eukaryotic methyltransferase (RomA) that methylates K14 of histone H3 (H3K14me3) to counteract host immune responses. However, it is not known howL. pneumophilainfection catalyses H3K14 methylation as this residue is usually acetylated. Here we show thatL. pneumophilasecretes a eukaryotic-like histone deacetylase (LphD) that specifically targets H3K14ac and works in synergy with RomA. Both effectors target host chromatin and bind the HBO1 histone acetyltransferase complex that acetylates H3K14. Full activity of RomA is dependent on the presence of LphD as H3K14 methylation levels are significantly decreased in a ΔlphDmutant. The dependency of these two chromatin-modifying effectors on each other is further substantiated by mutational and virulence assays revealing that the presence of only one of these two effectors impairs intracellular replication, while a double knockout (ΔlphDΔromA) can restore intracellular replication. Uniquely, we present evidence for “para-effectors”, an effector pair, that actively and coordinately modify host histones to hijack the host response. The identification of epigenetic marks modulated by pathogens opens new vistas for the development of innovative therapeutic strategies to counteract bacterial infection and strengthening host defences.
Opto-RhoGEFs: an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength
Authors: Mahlandt Eike K. , Martínez Sebastián Palacios , Arts Janine J. G. , Tol Simon , van Buul Jaap D. , Goedhart Joachim 
Published: October 2022

AbstractThe inner layer of blood vessels consists of endothelial cells, which form the physical barrier between blood and tissue. This vascular barrier is tightly regulated to allow the passage of essential molecules like oxygen, carbon-dioxide, water, ions, and nutrients. The vascular endothelial barrier is defined by cell-cell contacts through adherens and tight junctions. To further investigate the signaling in the endothelium that regulates vascular barrier strength, we focused on Rho GTPases, regulators of the actin cytoskeleton and known to control junction integrity. Rho GTPase signaling is confined in space and time. To manipulate the signaling in a temporal and spatial manner we applied optogenetics. Guanine exchange factor (GEF) domains from ITSN1, TIAM1 and p63RhoGEF, activating Cdc42, Rac and Rho respectively, were integrated into the optogenetic recruitment tool iLID. This tool allows for activation at the subcellular level in a reversible and non-invasive manner and thereby to recruit a GEF to local areas at the plasma membrane, enabling the local activation of specific Rho GTPases. The membrane tag of iLID was optimized and a HaloTag was applied to gain more flexibility for multiplex imaging. The resulting Opto-RhoGEFs were tested in an endothelial cell monolayer and demonstrated precise temporal control of vascular barrier strength by a cell-cell overlap-dependent, VE-cadherin-independent, mechanism. Furthermore, Opto-RhoGEFs enabled precise optogenetic control in endothelial cells over morphological features such as cell-size, -roundness, local extension, and cell contraction. In conclusion, we have optimized and applied the optogenetic iLID GEF recruitment tool i.e. Opto-RhoGEFs, to study the role of Rho GTPases in the vascular barrier of the endothelium and found that membrane protrusions at the junction region can rapidly increase barrier integrity independent of VE-cadherin.
Determining factors of continuance intention in mobile payment: fintech industry perspective
Authors: Laksamana Patria , Suharyanto Suharyanto , Cahaya Yohanes Ferry 
Published: October 2022

PurposeTo investigate consumer continuance intention in mobile payment in the financial technology (fintech) industry.Design/methodology/approachThis empirical study used an online survey with 673 responses from consumers, with structural equation modelling for data analysis.FindingsThe results revealed that trust, perceived usefulness, perceived ease of use, perceived risk and perceived security significantly affect consumer attitude. A positive impact on consumer attitude towards consumer engagement was also exposed.Research limitations/implicationsTrust, perceived usefulness, perceived ease of use, perceived risk and perceived security significantly affect consumer attitude. Hence, consumer attitude and consumer engagement have a positive influence on continuance intention.Practical implicationsThe study offers guidelines for decision makers to expand long-term engagement with consumers and enable continuance use of mobile payment services.Social implicationsThe findings will ultimately guide fintech firms in the implementation of a more secure macro financial system.Originality/valueThis study highlights the importance of consumer attitudes and engagement in mobile payment and extends the TAM model for more extensive technological advancements.