bluuebunny/crossref_metadata_2025_split huggingface dataset:
Submergence tolerance in immature stages of the stalk‐eyed fly Sphyracephala detrahens (Diptera: Diopsidae)
Authors: Kudo Ayumi 
Published: November 2022

AbstractSphyracephala detrahens (Walker, 1860) (Diptera: Diopsidae) inhabits the riparian zones of streams and rivers. Because of the limited dispersal ability of S. detrahens during egg, larval, and pupal stages, immature individuals are at risk of being submerged by floodwater after heavy rain. In this study, I evaluated the submergence tolerances of immatures of S. detrahens by comparing them to immatures of Drosophila melanogaster, which also feed on rotten fruits but are not restricted to the riparian zone. The results showed that S. detrahens eggs were susceptible to desiccation, but more than 80% of eggs hatched after full submergence. Later instar larvae were more resistant to full submergence than earlier instar larvae. The duration of submergence causing 50% pupation (PD50) in the first, second, and third‐instar larvae of S. detrahens were 15.88, 58.46, and 91.74 h, respectively. The PD50 of the third‐instar larvae of D. melanogaster was 20.01 h. Third‐instar S. detrahens larvae continued to develop in water for a longer duration than D. melanogaster larvae of the same instar. In the pupal stages, late pupae tended to remain afloat longer than early pupae. The duration of submergence causing 50% emergence (ED50) of adults from early and late pupae were 40.70 and 104.74 h, respectively. In the larval and pupal stages, individuals in the later developmental phases tended to be more tolerant to full submergence. The submergence tolerance of the immature stages of S. detrahens may reflect adaptation to an environment with fluctuating water levels.
Efficient Photoreactivation of Solar UV‐InjuredMetarhizium robertsii by Rad1 and Rad10 Linked to DNA Photorepair‐Required Proteins
Authors: Zhang Yi‐Lu , Peng Han , Ying Sheng‐Hua , Feng Ming‐Guang 
Published: November 2022

AbstractNucleotide excision repair (NER) of ultraviolet (UV)‐induced DNA lesions known as cyclobutane pyrimidine dimer (CPD) and (6–4)‐pyrimidine‐pyrimidone (6‐4PP) photoproducts depends on the activities of multiple anti‐UV radiation (RAD) proteins in budding yeast. However, NER remains poorly known in filamentous fungi, whose DNA lesions are photorepaired by one or two photolyases, namely CPD‐specific Phr1 and/or 6‐4PP‐specific Phr2. Previously, the white collar proteins WC1 and WC2 were proven to regulate expressions of phr2 and phr1 and photorepair 6‐4PP and CDP DNA lesions, respectively, in Metarhizium robertsii, a filamentous entomopathogenic‐phytoendophytic fungus. We report here high activities of orthologous Rad1 and Rad10 in 5‐h photoreactivation of UVB‐injured or UVB‐inactivated conidia but a severely compromised capability of their reactivating those conidia via 24‐h dark incubation in M. robertsii. The null mutants of rad1 and rad10 were much more compromised in conidial UVB resistance and photoreactivation capability than the previous null mutants of phr1, phr2, wc1 and wc2. Multiple protein–protein (Rad1‐Rad10, Rad1‐WC2, Rad10‐Phr1, WC1‐Phr1/2 and WC2‐Phr1/2) interactions detected suggest direct/indirect links of Rad1 and Rad10 to Phr1/2 and WC1/2 and an importance of the links for their photoreactivation activities. Conclusively, Rad1 and Rad10 photoreactivate UVB‐impaired M. robertsii through their interactions with the DNA photorepair‐required proteins.
Intraspecific variation in morphology of spiny pollen grains along an altitudinal gradient in an insect‐pollinated shrub
Authors: Hasegawa T. M. , Itagaki T. , Sakai S. 
Published: November 2022

ABSTRACT
Intraspecific variations in pollen morphological traits are poorly studied. Interspecific variations are often associated with pollination systems and pollinator types. Altitudinal environmental changes, which can influence local pollinator assemblages, provide opportunities to explore differentiation in pollen traits of a single species over short distances. The aim of this study is to examine intraspecific variations in pollen traits of an insect‐pollinated shrub, Weigela hortensis (Caprifoliaceae), along an altitudinal gradient.
Pollen spine phenotypes (length, number and density), pollen diameter, lipid mass (pollenkitt) around pollen grains, pollen production per flower and pollinator assemblages were compared at four sites at different altitudes.
Spine length and the spine length/diameter ratio of pollen grains were greater at higher altitudes but not correlated with flower or plant size. Spine number and density increased as flower size increased, and pollen lipid mass decreased as plant size increased. Bees were the predominant pollinators at low‐altitude sites whereas flies, specifically Oligoneura spp. (Acroceridae), increased in relative abundance with increasing altitude.
The results of this study suggest that the increase in spine length with altitude was the result of selection favouring longer spines at higher‐altitude sites and/or shorter spines at lower‐altitude sites. The altitudinal variation in selection pressure on spine length could reflect changes in local pollinator assemblages with altitude.
Behavioral consequences of intergroup sensitivity
Authors: Thürmer J. Lukas , McCrea Sean M. 
Published: November 2022

AbstractCommunication of criticism between groups is important to productive societal discourse, but may serve to inflame conflict. The defensive rejection of intergroup, relative to intragroup, criticism (intergroup sensitivity effect [ISE]) may contribute to such divides. The ISE has been observed in self‐report measures, but such measures are weak and biased predictors of actual (divisive) behavior. We review recent research demonstrating that the ISE does have costly behavioral consequences. We next consider the debate concerning whether the ISE reflects defense of a valued social identity or enforcement of a conversational norm. We therefore review recent evidence for an ISE among individuals lacking identification with the target of the criticism. Third, we examine how the ISE may affect a variety of behaviors relevant to societal divisions, including political reconciliation, environmental sustainability, and vaccination. Lastly, we outline future research that can clarify the underlying mechanisms of the ISE and promote conciliatory intergroup behavior.
Immature Vocalizations Simplify the Speech of Tseltal Mayan and U.S. Caregivers
Authors: Elmlinger Steven L. , Goldstein Michael H. , Casillas Marisa 
Published: November 2022

AbstractWhat is the function of immature vocalizing in early learning environments? Previous work on infants in the United States indicates that prelinguistic vocalizations elicit caregiver speech which is simplified in its linguistic structure. However, there is substantial cross‐cultural variation in the extent to which children's vocalizations elicit responses from caregivers. In the current study, we ask whether children's vocalizations elicit similar changes in their immediate caregivers’ speech structure across two cultural sites with differing perspectives on how to interact with infants and young children. Here, we compare Tseltal Mayan and U.S. caregivers’ verbal responses to their children's vocalizations. Similar to findings from U.S. dyads, we found that children from the Tseltal community regulate the statistical structure of caregivers’ speech simply by vocalizing. Following the interaction burst hypothesis, where clusters of child‐adult contingent response alternations facilitate learning from limited input, we reveal a stable source of information that may facilitate language learning within ongoing interaction.
Sinapis genomes provide insights into whole‐genome triplication and divergence patterns within tribe Brassiceae
Authors: Yang Taihua , Cai Bowei , Jia Zhibo , Wang Yu , Wang Jing , King Graham J , Ge Xianhong , Li Zaiyun 
Published: November 2022

SUMMARYSinapis alba and Sinapis arvensis are mustard crops within the Brassiceae tribe of the Brassicaceae family, and represent an important genetic resource for crop improvement. We performed the de novo assembly of Brassica nigra, S. alba, and S. arvensis, and conducted comparative genomics to investigate the pattern of genomic evolution since an ancient whole‐genome triplication event. Both Sinapis species retained evidence of the Brassiceae whole‐genome triplication approximately 20.5 million years ago (Mya), with subgenome dominance observed in gene density, gene expression, and selective constraint. While S. alba diverged from the ancestor of Brassica and Raphanus at approximately 12.5 Mya, the divergence time of S. arvensis and B. nigra was approximately 6.5 Mya. S. arvensis and B. nigra had greater collinearity compared with their relationship to either Brassica rapa or Brassica oleracea. Two chromosomes of S. alba (Sal03 and Sal08) were completely collinear with two ancestral chromosomes proposed in the Ancestral Crucifer Karyotype (ACK) genomic block model, the first time this has been observed in the Brassiceae. These results are consistent with S. alba representing a relatively ancient lineage of the species evolved from the common ancestor of tribe Brassiceae, and suggest that the phylogeny of the Brassica and Sinapis genera requires some revision. Our study provides new insights into the genome evolution and phylogenetic relationships of Brassiceae and provides genomic information for genetic improvement of these plants.
An observational study of the blood use in combat casualties of the French Armed Forces, 2013–2021
Authors: Py Nicolas , Pons Sandrine , Boye Matthieu , Martinez Thibault , Ausset Sylvain , Martinaud Christophe , Pasquier Pierre 
Published: November 2022

AbstractBackgroundThe French Armed Forces conduct asymmetric warfare in the Sahara‐Sahel Strip. Casualties are treated with damage control resuscitation to the extent possible. Questions remain about the feasibility and sustainability of using blood for wider use in austere environments.MethodsWe performed a retrospective analysis of all French military trauma patients transfused after injury in overseas military operations in Sahel‐Saharan Strip, from the point of injury, until day 7, between January 11, 2013 to December 31, 2021.ResultsForty‐five patients were transfused. Twenty‐three (51%) of them required four red blood cells units (RBC) or more in the first 24H defining a severe hemorrhage. The median blood product consumption within the first 48 h, was 8 (IQR [3; 18]) units of blood products (BP) for all study population but up to 17 units (IQR [10; 27.5]) for the trauma patients with severe hemorrhage. Transfusion started at prehospital stage for 20 patients (45%) and included several blood products: French lyophilized plasma, RBCs, and whole blood. Patients with severe hemorrhage required a median of 2 [IQR 0; 34] further units of BP from day 3 to day 7 after injury. Eight patients died in theater, 4 with severe hemorrhage and these 4 used an average of 12 products at Role 1 and 2.ConclusionThe transfusion needs were predominant in the first 48 h after the injury but also continued throughout the first week for the most severe trauma patients. Importantly, our study involved a low‐intensity conflict, with a small number of injured combatants.
Integrated sequential fuzzy logic search models for simulating wastewater treatment plants missing influent parameters
Authors: Abunama Taher , Seyam Mohammed , Ansari Mozafar , Kumari Sheena , Bux Faizal 
Published: November 2022

AbstractAiming at achieving optimum operations of wastewater treatment plant (WWTP) processes, it is essential to develop accurate predictive models for expected influent pollutant loads. To overcome limited timespan data and model complexity, this study is devoted to propose an integration of inputs' sequential search with the adaptive neuro‐fuzzy inference system (ANFIS) for reducing the modelling complexity. The input data included nine influent parameters measured bi‐weekly for 12 years at a WWTP, South Africa (SA). The sequential search process was used for input optimization to select the most representative inputs in modelling four parameters. The obtained results indicated a strong correlation with R2 and NSE above 0.85 for the four targeted influent parameters. After validating the developed models using different criteria, the missing records were predicted throughout the study period. The integration of sequential search input optimization and ANFIS modelling was able to provide a high performance in modelling WWTP datasets.
Five-year follow-up of patients with knee osteoarthritis not eligible for total knee replacement: results from a randomised trial
Authors: Larsen Jesper Bie , Roos Ewa M , Laursen Mogens , Holden Sinead , Johansen Martin Nygaard , Rathleff Michael Skovdal , Arendt-Nielsen Lars , Rasmussen Sten , Skou Søren T 
Published: November 2022

ObjectivesThe main objective was to investigate 5-year outcomes in patients with knee osteoarthritis, randomised to one of two non-surgical treatments.SettingTwo outpatient clinics.ParticipantsAt baseline, 100 patients with radiographic and symptomatic knee osteoarthritis not found eligible for knee replacement (KR) were included. Main exclusion criteria were average score above 75 of the Knee injury and Osteoarthritis Outcome Score (KOOS) subscales pain, symptoms, function of daily living and quality of life; KOOS4and average knee pain the previous week greater than 60 mm on a 100 mm visual analogue scale.InterventionsPatients were randomised to supervised non-surgical treatment consisting of patient education, supervised exercise, weight loss, insoles, and pain medication (the MEDIC treatment) or written advice. The 12-week MEDIC treatment included patient education, neuromuscular exercise, insoles and a dietary weight loss programme and/or pain medication if needed and written advice consisted of two leaflets.Primary and secondary outcome measuresPrimary outcome was 5-year mean change for KOOS4. Secondary outcomes included KOOS subscales, self-reported health, usage of pain medication and self-reported physical activity.ResultsThirty-nine (78%) and 36 (72%) from the MEDIC and written advice groups responded at 5 years. There were no between-group differences in KOOS4(difference 5.3 (95% CI −1.5 to 12.1) or any secondary outcomes. However, the 95% CI included the minimal clinically important difference for the main outcome.Seventy-six percent of the MEDIC group and 66% of the written advice group experienced clinically important improvements in KOOS4.Fifteen patients (30%) from the MEDIC group and 17 (34%) from the written advice group received KR in the index knee. Undergoing KR did not result in a statistically significant greater improvement in KOOS4(difference 6.1 (95% CI −1.1 to 13.4).ConclusionsNo statistically significant differences between supervised non-surgical treatment and written advice were demonstrated at 5 years. Most patients experienced clinically important improvements, irrespective of initial treatment strategy or KR.Trial registration numberNCT01535001; ClinicalTrials.gov.
From guidelines to practice: A retrospective clinical cohort study investigating implementation of the early detection guidelines for cerebral palsy in a state-wide early intervention service
Authors: Davidson Sue-Anne , Ward Roslyn , Elliott Catherine , Harris Courtenay , Bear Natasha , Thornton Ashleigh , Salt Alison , Valentine Jane 
Published: November 2022

ObjectivesTo report on knowledge translation strategies and outcomes from the implementation of the early detection guidelines for cerebral palsy (CP) in a state-wide tertiary early intervention (EI) service and investigate the impact of social determinants on clinical services.DesignRetrospective longitudinal cohort study.SettingThe Western Australia tertiary paediatric EI service.ParticipantsEI clinicians, consumers and children using the EI service.Outcome measuresKnowledge translation strategies including consumer perspectives, clinician training and Communities of Practice (CoP) guided implementation. We measured changes in referral number and age, delivery of early detection and intervention following the implementation of the guidelines. Exposure to adverse childhood experiences (ACEs), appointment non-attendance (DNA) rates, remoteness and socioeconomic quintiles were used to measure social determinants of health using negative binomial (Incidence Rate Ratios, IRR) and logistic regression (Odds Ratios, ORs).ResultsTen consumers participated in Focus Groups, 100 clinicians were trained and 22 clinicians established a monthly CoP. Referrals increased fourfold to 511 children. Corrected gestational age at referral decreased from a median of 16.1 to 5.1 months (p<0.001) and at first appointment from 18.8 to 6.8 months (p<0.001). Children living in social disadvantage had the highest DNA risk (quintile 1 vs 5: IRR 2.2, 95% CI 1.1 to 4.6, p=0.037). Children exposed to ACEs had higher odds of living in social disadvantage (quintile 1 vs 5, OR=3.8, 95% CI 1.4 to 10.0, p=0.007). No significant association was found between remoteness and DNA rate or ACE score.ConclusionsImplementation strategies reduced referral age and improved the delivery of early detection assessments. Further investigation of the association between social disadvantage, DNA risk and ACE score is required in the development of a state-wide early detection network.
Impact of kinesiophobia on initiation of cardiac rehabilitation: a prospective cohort path analysis
Authors: Keessen Paul , Kan Kees-Jan , Ter Riet Gerben , Visser Bart , Jørstad Harald , Latour Corine , van Duijvenbode Ingrid , Scholte op Reimer Wilma 
Published: November 2022

ObjectivesTo identify factors associated with kinesiophobia (fear of movement) after cardiac hospitalisation and to assess the impact of kinesiophobia on cardiac rehabilitation (CR) initiation.DesignProspective cohort study.SettingAcademic Medical Centre, Department of Cardiology.ParticipantsWe performed a prospective cohort study in cardiac patients recruited at hospital discharge. In total, 149 patients (78.5% male) with a median age of 65 years were included, of which 82 (59%) were referred for CR.Primary and secondary outcome measuresWe assessed kinesiophobia with the Tampa Scale for Kinesiophobia (TSK). For this study, the total score was used (range 13–52). We assessed baseline factors (demographics, cardiac disease history, questionnaire data on anxiety, biopsychosocial complexity and self-efficacy) associated with kinesiophobia using linear regression with backward elimination. For linear regression, the standardised beta (β) was reported. Prospectively, the impact of kinesiophobia on probability of CR initiation, in the first 3 months after hospital discharge (subsample referred for CR), was assessed with logistic regression. For logistic regression, the OR was reported.ResultsModerate and severe levels of kinesiophobia were found in 22.8%. In the total sample, kinesiophobia was associated with cardiac anxiety (β=0.33, 95% CI: 0.19 to 0.48), social complexity (β=0.23, 95% CI: 0.06 to 0.39) and higher education (β=−0.18, 95% CI: −0.34 to −0.02). In those referred for CR, kinesiophobia was negatively associated with self-efficacy (β=−0.29, 95% CI: −0.47 to −0.12) and positively with cardiac anxiety (β=0.43, 95% CI: 0.24 to 0.62). Kinesiophobia decreased the probability of CR initiation (ORRange13–52 points=0.92, 95% CI: 0.85 to 0.99).ConclusionIn patients hospitalised for cardiovascular disease, kinesiophobia is associated with cardiac anxiety, social complexity, educational level and self-efficacy. Kinesiophobia decreased the likelihood of CR initiation with 8% per point on the TSK.
Health service use before and after dementia diagnosis: a retrospective matched case–control study
Authors: Kosteniuk Julie , Osman Beliz Açan , Osman Meric , Quail Jacqueline M , Islam Naorin , O'Connell Megan E , Kirk Andrew , Stewart Norma J , Morgan Debra 
Published: November 2022

ObjectivesThis study investigated patterns in health service usage among older adults with dementia and matched controls over a 10-year span from 5 years before until 5 years after diagnosis.DesignPopulation-based retrospective matched case–control study.SettingAdministrative health data of individuals in Saskatchewan, Canada from 1 April 2008 to 31 March 2019.ParticipantsThe study included 2024 adults aged 65 years and older living in the community at the time of dementia diagnosis from 1 April 2013 to 31 March 2014, matched 1:1 to individuals without a dementia diagnosis on age group, sex, rural versus urban residence, geographical region and comorbidity.Outcome measuresFor each 5-year period before and after diagnosis, we examined usage of health services each year including family physician (FP) visits, specialist visits, hospital admissions, all-type prescription drug dispensations and short-term care admissions. We used negative binomial regression to estimate the effect of dementia on yearly average health service utilisation adjusting for sex, age group, rural versus urban residence, geographical region, 1 year prior health service use and comorbidity.ResultsAdjusted findings demonstrated that 5 years before diagnosis, usage of all health services except hospitalisation was lower among persons with dementia than persons without dementia (all p<0.001). After this point, differences in higher health service usage among persons with dementia compared to without dementia were greatest in the year before and year after diagnosis. In the year before diagnosis, specialist visits were 59.7% higher (p<0.001) and hospitalisations 90.5% higher (p<0.001). In the year after diagnosis, FP visits were 70.0% higher (p<0.001) and all-type drug prescriptions 29.1% higher (p<0.001).ConclusionsFindings suggest the year before and year after diagnosis offer multiple opportunities to implement quality supports. FPs are integral to dementia care and require effective resources to properly serve this population.
Modified Erasmus GBS Respiratory Insufficiency Score: a simplified clinical tool to predict the risk of mechanical ventilation in Guillain-Barré syndrome
Authors: Luijten Linda W G , Doets Alex Y , Arends Samuel , Dimachkie Mazen M , Gorson Kenneth C , Islam Badrul , Kolb Noah A , Kusunoki Susumu , Papri Nowshin , Waheed Waqar , Walgaard Christa , Yamagishi Yuko , Lingsma Hester , Jacobs Bart C 
Published: November 2022

BackgroundThis study aimed to determine the clinical and diagnostic factors associated with mechanical ventilation (MV) in Guillain-Barré syndrome (GBS) and to simplify the existing Erasmus GBS Respiratory Insufficiency Score (EGRIS) for predicting the risk of MV.MethodsData from the first 1500 patients included in the prospective International GBS Outcome Study (IGOS) were used. Patients were included across five continents. Patients <6 years and patients from Bangladesh were excluded. Univariable logistic and multivariable Cox regression were used to determine which prespecified clinical and diagnostic characteristics were associated with MV and to predict the risk of MV at multiple time points during disease course.Results1133 (76%) patients met the study criteria. Independent predictors of MV were a shorter time from onset of weakness until admission, the presence of bulbar palsy and weakness of neck flexion and hip flexion. The modified EGRIS (mEGRIS) was based on these factors and accurately predicts the risk of MV with an area under the curve (AUC) of 0.84 (0.80–0.88). We internally validated the model within the full IGOS cohort and within separate regional subgroups, which showed AUC values of 0.83 (0.81–0.88) and 0.85 (0.72–0.98), respectively.ConclusionsThe mEGRIS is a simple and accurate tool for predicting the risk of MV in GBS. Compared with the original model, the mEGRIS requires less information for predictions with equal accuracy, can be used to predict MV at multiple time points and is also applicable in less severely affected patients and GBS variants. Model performance was consistent across different regions.