bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of hospitalized patients with severe pneumonia caused by COVID-19 and influenza A (H7N9 and H1N1): A retrospective study from a designated hospital
Authors: Gu Binbin , Yao Lin , Zhu Xinyun , Tang Peijun , Chen Cheng 
Published: December 2022

Abstract
Considerable attention has been focused on the clinical features of coronavirus disease 2019 (COVID-19), but it is also important for clinicians to differentiate it from influenza virus infections. In the present study, the rate of coexisting disease was lower in the severe COVID-19 group than in the influenza A group (p = 0.003). Radiologically, severe COVID-19 patients had fewer instances of pleural effusion (p < 0.001). Clinically, severe COVID-19 patients had relatively better disease severity scores, less secondary bacterial infections, shorter times to beginning absorption on computed tomography, but longer durations of viral shedding from the time of admission (p < 0.05). Although the more severe influenza A patients required noninvasive respiratory support, these two groups ultimately yielded comparable mortalities. Based on the multiple logistic regression analysis, severe COVID-19 infection was associated with a lower risk of severe acute respiratory distress syndrome [odds ratio (OR) 1.016, 95% [confidence interval (CI)] 1.001–1.032, p = 0.041] and a better pneumonia severity index (OR 0.945, 95% [CI] 0.905−0.986, p = 0.009); however, these patients exhibited longer durations of viral shedding (OR 1.192, 95% [CI] 1.047−1.357, p = 0.008) than patients with severe influenza A infection. In conclusion, the conditions of severe influenza A patients appeared to be more critical than that of severe COVID-19 patients. However, relatively lower mortalities of these two severe cases are expected in the context of sufficient medical supplies.
Local existence and blowing up phenomena for a class of non-autonomous partial functional differential equations with infinite delay
Authors: Diop Mamadou Abdoul , Ezzinbi Khalil , Kyelem Bila Adolphe 
Published: December 2022

Abstract
In this work, we study a class of abstract non-autonomous partial functional differential equations with infinite delay. Our main results concern the local existence of the mild solution which can blow up at the finite time. The unbounded operators associated to the non-autonomous system are assumed to be stable family which generates C
0-semigroups while the nonlinear part is supposed to be continuous. Under Lipschitz condition on the nonlinear term of the equation, we prove the existence and uniqueness of the mild solution. For illustration, we provide an example for some reaction-diffusion non-autonomous partial functional differential equations involving infinite delay.
Experimental investigation into paper dust formation during knife edge cutting on a laboratory scale
Authors: Lunewski Johannes , Schmidt Eberhard 
Published: December 2022

AbstractEspecially in the field of paper and print post-processing on an industrial scale, continuous cutting operations generate paper dust. Despite preventive measures like dedusting units, a high paper web velocity induces the release of particulate dust. As a result, the particles settle and accumulate on machine components and in the surrounding area. Resuspended particles around a potential ignition source represent a fire hazard. The prevention of such fire incidents requires frequent servicing and maintenance. However, the shutdown time on industrial scale applications remains cost-intensive.In this research, a cutting plotter executes reproducible knife edge cutting sequences to identify significant parameters affecting the paper dust formation on a macro and micro scale. For this purpose, an extraction system collects the exposed particles along the cutting line. The applied methods include gravimetric determination of the dust mass and dynamic image analysis to characterise particle size and shape. Final results indicate a significant influence of the blade wear condition.
New perspectives on fertility in transwomen with regard to spermatogonial stem cells
Authors: Dabel Jennifer , Schneider Florian , Wistuba Joachim , Kliesch Sabine , Schlatt Stefan , Neuhaus Nina 
Published: December 2022

Objective
Germ cells of transwomen are affected by gender-affirming hormone therapy (GAHT). Fertility will be lost after surgical intervention; thereby, fertility preservation becomes an increasingly imortant topic. This study investigated if the absolute number of spermatogonia in transwomen is comparable at the time of gender-affirming surgery (GAS) to that in pre-pubertal boys.

Methods
We carried out a retrospective study of testicular tissues from 25 selected subjects, which had undergone a comparable sex hormone therapy regimen using cyproterone acetate (10 or 12.5 mg) and estrogens. As controls, testicular biopsies of five cisgender adult men (aged 35–48 years) and five pre-/pubertal boys (5–14 years) were included. Testicular tissues were immunohistochemically stained for MAGE A4-positive cells, the most advanced germ cell type. The number of spermatogonia per area was assessed. Clinical values and serum hormone values for FSH, LH, testosterone, free testosterone, estradiol and prolactin were determined on the day of GAS for correlation analyses.

Results
Round spermatids were the most advanced germ cell type in 3 subjects, 5 had an arrest at spermatocyte stage, while 17 showed a spermatogonial arrest. On average, testicular tissues of transwomen contained 25.15 spermatogonia/mm3, a number that was significantly reduced compared to the two control groups (P < 0.01, adult 80.65 spermatogonia/mm3 and pre-/pubertal boys 78.55
spermatogonia/mm3). Linear regression analysis revealed that testes with higher weight and high LH contained more spermatogonia.

Conclusion
Irrespective of treatment dose or duration, spermatogenesis was impaired. Spermatogonial numbers were significantly reduced in transwomen compared to the control groups.

Lay summary
When transwomen go through treatment to confirm their gender, their germ cells are affected. They lose their fertility after surgery, so fertility preservation becomes an important topic. We carried out a study looking at tissue from testes of 25 people who had been through the same sex hormone therapy until surgery. Blood samples were also taken. As controls, samples were taken from the testes of cisgender boys and adult men. On average, the samples from the testes of transwomen contained a smaller number of early sperm cells compared to the two control groups. Regardless of the dose or length of hormone treatment, the fertility of transwomen was significantly reduced so that counseling about fertility preservation should be offered before hormone therapy.
Variabilidad morfológica/patogénica de Fusarium verticillioides en la Ciénega/Chapala, México y evaluación de técnicas de inoculación
Authors: Zácamo-Velázquez Norma Y. , Ireta-Moreno Javier , Salinas-Moreno Yolanda , Gómez-Rodríguez Víctor M. , Ramírez-Vega Humberto , Martínez-Loperena Raquel 
Published: December 2022

Introducción. La pudrición de mazorca en maíz causada por Fusarium verticillioides es una enfermedad de las más importantes en el mundo. En México ocasiona pérdidas de hasta 30 %. El incremento en los niveles de esta enfermedad en los últimos años requiere inspecciones en las zonas productoras para identificar las principales cepas que afectan el grano de maíz. Objetivo. Determinar la variabilidad morfológica y patogénica de aislados de F. verticillioides de muestras de maíz de la zona productora de maíz La Ciénega/Chapala, México, e identificar la mejor técnica de inoculación del patógeno en mazorcas. Materiales y métodos. Se obtuvieron veinte aislamientos de F. verticillioides de muestras de granos de maíz con síntomas de la enfermedad. Los aislamientos se usaron para inocular, mediante diferentes técnicas, mazorcas en desarrollo (R2) del maíz P-3057 cultivado en Tepatitlán, Jalisco en el ciclo Primavera/Verano 2019 bajo condiciones de temporal. Se evaluó la incidencia y severidad de la infección (SDI) de los aislamientos y la morfología microscópica de las cepas. Resultados. Los aislamientos de F. verticillioides difirieron en color de la colonia, tipo de micelio, abundancia y coloración del micelio vegetativo. La incidencia y severidad fueron estadísticamente diferentes entre los aislamientos de F. verticillioides y las técnicas de inoculación probadas, con interacción entre aislamientos y técnicas de inoculación. La incidencia de la enfermedad mostró valores de 82 y 95 % con las técnicas de inoculación por aspersión y del palillo, respectivamente. La SDI varió entre 8 % y 15,5 % con las técnicas de inoculación por aspersión y palillo, en ese orden. Conclusiones. Las cepas de F. verticillioides mostraron variabilidad para patogenicidad y severidad de la infección. La patogenicidad se relacionó con la técnica de inoculación utilizada. La técnica de inoculación del palillo fue la que presentó mayor incidencia y severidad de la infección.
The Hungarian sword of constitutional identity
Authors: Várnay Ernő 
Published: December 2022

AbstractIn its ‘refugee quota decision’ of 2016 the Hungarian Constitutional Court (HCC) ‘invented’ its competences of ultra vires, sovereignty and constitutional identity controls. The sword of constitutional identity (CI) has been forged against foreign – first of all – EU law. In the development of the new concept the interplay between the Government, the Government-dominated parliament and the Constitutional Court loyal to the Government seems to be evident. The textual analysis of the relevant HCC's decisions proves that the Hungarian Constitutional identity (HCI) contains legal acts in force – including the Fundamental Law (constitution) and the Founding treaties of the EU -, legal acts ‘not in force but valid’ and activities related to the fight for independence of the Hungarian State.As far as the nature of the HCI is concerned, the article demonstrates the strong relationship with sovereignty control, and the ‘historical constitution’ and emphasises the HCC's statement according to which the CI is not created by the constitution, it is merely acknowledges it.Given the large number of elements identified as part of the HCI, its openness to the inclusion of further elements, and the questionable nature of the HCI, the author submits that the concept is inappropriate for any meaningful constitutional review.The HCC – at least until now – despite being invited to do so, has refused to use the sword against EU secondary law and the judgment of the European Court of Justice, and avoided overt constitutional conflict. However, this does not mean that the HCC is ready to enter into sincere dialogue with the court in Luxembourg.
Ernő Dohnányi: A Discography of the Performer
Authors: Szabó Ferenc János 
Published: December 2022

AbstractThe performances of Ernő Dohnányi as pianist and conductor were preserved on numerous sound recordings. He was involved in the recording industry first in 1905, and his death was notoriously caused by a cold suffered in a recording studio in 1960. His interpretation is preserved on different audio media: piano rolls, 78rpm and Long Play discs, x-ray foils and reel-to-reel tapes. Although the number of his studio recordings, made for commercial purposes, is relatively small, the amount of live concert and home recordings, including the huge collection of unpublished recordings made in the USA between 1945 and 1960, expands it to a significant corpus of sound recordings. This article contains the complete discography of Ernő Dohnányi as a performer. The discography provides all available data of the studio and live recordings of Dohnányi, including the data of reissues (closing date: June 2022). It is preceded by an article in which Dohnányi's discography is analysed from several aspects. The analysis of the recorded repertoire sets the stage for further research on Dohnányi's interpretation; however, lost recordings are also reviewed. Dohnányi's controversial relationship to the technical media, and vice versa the recording firms changing interest in him as a performer, are also discussed in detail, involving several sources formerly unknown to Dohnányi research.
SOSIALISASI PROGRAM STICOM DAN PRAKTIK BUDIKDAMBER DI KAMPUNG MAKBUSUM, DISTRIK MAYAMUK, KABUPATEN SORONG
Authors: Ernawati Ernawati , Poltak Hendra , Ruzuqi Rezza , Abadi Agung Setia , Puspitasari Asthervina Widyastami , Hismayasari Intanurfemi Bacandra , Kadarusman Kadarusman , Ziliwu Boby W , Prakoso Bagas 
Published: December 2022

STICOM (Smart Tilapia Community) merupakan program yang digagas oleh prodi. Teknik Budidaya Perikanan Politeknik Kelautan dan Perikanan Sorong dengan bekerjasama dengan pihak Balai Benih Ikan Majaran. Kegiatan tersebut diharapkan meningkatkan kesadaran masyarakat tentang pemanfaatan lahan atau area sempit untuk berbudidaya ikan sekaligus sayur yang efektif dalam ember. Hal tersebut nantinya diharap juga dapat menambah penghasilan warga distrik mayamuk. Kegiatan dilaksanakan menggunakan metode sosialisasi STICOM dan praktik pembuatan BUDIKDAMBER (budidaya ikan dalam ember). Kegiatan sosialisasi mengundang warga distrik mayamuk. Setelah kegiatan tersebut dilakukan, selanjutnya dilakukan survey kepuasan terhadap kegiatan tersebut. Hasil yang didapatkan yakni masyarakat mayamuk merasa puas terhadap kegiatan yang telah dilakukan oleh tim dan berharap kegiatan tersebut dilakukan ulang guna dilakukan evaluasi kegiatan.
Effectiveness of neoton in patients with chronic heart failure depending on the left ventricular ejection fraction. Sub-analysis of the BYHEART study
Authors: Tereshchenko S. N. , Cheremisina I. A. , Safiullina A. A. 
Published: December 2022

Aim. To study the effectiveness of exogenous phosphocreatine (EPCr) therapy in patients with heart failure (HF) with reduced and mildly reduce dejection fraction (EF).Material and methods. The all-Russian prospective observational study BYHEART included 842 patients who underwent aEPCr therapy course. Before and after the course of EPCr therapy, the following studies were carried out: a questionnaire on the Minnesota Living with Heart Failure Questionnaire (MLHFQ) and the clinical assessment scale (CAS), transthoracic echocardiography with an assessment of the left ventricular EF, a 6-minute walk test, N-terminal pro-brain natriuretic peptide (NT-proBNP). All patients before the EPCr course received long-term optimal therapy for HF.Results. Data analysis was carried out for patients in groups with heart failure with reduced ejection fraction (HFrEF) and with mildly reduced ejection fraction (HFmrEF) within the group depending on the course of EPCr treatment: subgroup A — patients who received EPCr therapy less than 20 g/course and subgroup B — patients who received EPCr therapy ≥20 g/course. The results obtained demonstrate an increase in the quality of life and exercise tolerance, an improvement according to the CAS, a decrease in NT-proBNP levels, and an improvement in left ventricular systolic function in patients with both HFrEF and HFmrEF during the course of intravenous EPCr therapy.Conclusion. The use of EPCr is a promising tool in addition to optimal therapy in patients with HFrEF and HFmrEF.
NUMERICAL INVESTIGATION OF HVAF-SPRAYED Fe-BASED AMORPHOUS COATINGS
Authors: Wu Nianchu , Li Tingting , Lian Jingbao 
Published: December 2022

A numerical analysis was performed to predict the effect of the convergent section geometry of a gun nozzle on the high-velocity air-fuel (HVAF) thermal spray Fe-based amorphous coating (AC) process. A computational fluid dynamics model was applied to investigate the gas-flow field and the behavior of in-flight particles at nozzle entrance convergent section length ranging from 28 mm to 56.8 mm and different shapes of the Laval nozzle convergent section (a straight line and Vitosinski convergence curve). On the one hand, the change in the gas-flame flow characteristics for the Vitosinski curve shows a uniform and stable flame compared with the straight-line curve in the convergent section. The straight-line curve shape of the Laval nozzle convergent section has a higher particle temperature compared with the Vitosinski-curve shape of the Laval nozzle convergent section. The particle dwell time for the straight-line curve shape of the Laval nozzle convergent section is longer than that for the Vitosinski curve shape of the Laval nozzle convergent section. On the other hand, the nozzle entrance convergent section length obviously affects the particle temperature, and the particle dwell time increases with the increasing nozzle entrance convergent section length. By analyzing both the melt status of the particles and particle velocity, the optimal gun configuration (0.7 V) producing low-porosity coatings was predicted. These calculations were experimentally verified by producing a low-porosity (1.37 %) Fe-based AC, fabricated with HVAF using the predicted optimal gun configuration.
Un franciscano asturiano en Nueva España: fray Juan Agustín Morfi (1735-1783), Oviedo, IFESXVIII / Ediciones Trea (ACESXVIII, 2), 2018
Authors: Cadez Ortola Émilie 
Published: December 2022

Este trabajo presenta resultados parciales de la tesis doctoral Les mobilités intercontinentales dans le Royaume d’Espagne: fray Juan Agustín Morfi, franciscain asturien en Nouvelle-Espagne (1735-1783), defendida en la Universidad de Toulouse en 2017, y aspira a reordenar ciertos contenidos y aportar nuevos elementos que contribuyan al mejor conocimiento de la biografía y el perfil, tanto vital como intelectual, de Juan Agustín Morfi.
Identification of SNPs and InDels probably associated with the development of spontaneous tumors in radish (<i>Raphanus sativus</i> L.)
Authors: Kuznetsova Ksenia  A. , Dodueva Irina E. , Lutova Lyudmila A. 
Published: December 2022

Radish (Raphanus sativus L.) is an agronomically important root crop belonging to the Brassicaceae family. A genetic collection of inbred radish lines, which was based on contrasting genetically determined traits including trait ability to develop tumors, was created at St. Petersburg State University in the middle of the 20th century [1]. Full genetics network controlling this trait still remains unclear [2], and elucidation of its mechanisms can help reveal the key regulators of systemic mechanisms that control cell proliferation and differentiation.
The purpose of this work is to identify single nucleotide polymorphisms (SNPs) and InDels in genes which are candidates for participation in the tumor development process within in the tumor-forming radish line compared to the non-tumor radish line.
We have assembled the genomes of two lines contrasting in the ability to tumorigenesis, annotated them, aligned sequences per assembly, identified candidate genes and differences in the structure of these genes in contrasting radish lines using bioinformatics tools. Bioinformatics data were confirmed by the sequencing by Sanger method.
As a result, in the tumor-forming radish line we identified 151 genes with InDels in their coding regions, which led to various variants of frameshift. Moreover, we detected 39 genes with single nucleotide substitutions (SNPs). According to the gene pathway enrichment analysis, the corresponding genes were classified into the several groups.
Thus, the data obtained will allow us to clarify in more detail the genetic control mechanisms of the tumor development in radish.
The work was supported by the Ministry of Science and Higher Education of the Russian Federation in accordance with the agreement No. 075-15-2022-322.
The <i>MtWOX</i> and <i>MtCLE</i> genes in the regulation of <i>Medicago truncatula</i> somatic embryogenesis
Authors: Efremova Elena  P. , Tvorogova Varvara  E. , Lutova Ludmila  A. 
Published: December 2022

Plant somatic cells can be reprogrammed into totipotent embryonic cells that are able to form differentiated embryos in a process called somatic embryogenesis (SE). SE can occur naturally in various plant species and it is widely used for clonal propagation, transformation and regeneration of different crops. This process is regulated by hormone treatment and many proteins, among which WUSCHEL-related homeobox (WOX) transcription factors are believed to play crucial roles. The WOX family is involved in the regulation of a wide range of key developmental programs in different plant organs and tissues. CLE peptides are well-known hormonal regulators of plant development. WOX and CLE genes can be related to each other through feedback regulatory loops.
Our previous studies have shown that MtWOX9-1 stimulates SE in Medicago truncatula [1] and overexpression of the MtWOX9-1 gene increases the expression level of the MtCLE08, MtCLE16, MtCLE18 genes in SE. In this study, we examine the overexpression effect of MtCLE08, 16, and 18 on the expression level of MtWOX9-1 gene and a number of other MtWOX genes, which were shown to change expression in SE according to the transcriptomic data. No significant impact of MtCLE overexpression on any MtWOX gene under study was found.
Our findings could be a helpful point for searching and studying new morphogenetic regulators controlling SE and could have a positive impact on plant biotechnology in improving the transformation and regeneration capacity for other legumes.
The research was supported by grant from the Russian Foundation for Basic Research No. 20-016-00124.
<i>Komagataella phaffii</i> yeast as a model organism in biotechnology and fundamental research
Authors: Rumyantsev Andrey M. , Sidorin Anton V. , Ianshina Tatiana M. , Petrova Kristina D. , Ishtuganova Valeriia V. , Sambuk Elena V. , Padkina Marina V. 
Published: December 2022

The choice of a model organism in biology is based on various factors, such as practical significance and ease of manipulation. Saccharomyces cerevisiae yeast is one of the most widely used and well-studied eukaryotic models. However, developments in NGS, proteomics, metabolomics and gene editing methods allow other species to become the object of fundamental research. A good example of such emerging model organisms is the yeast Komagataella phaffii.
K. phaffii belongs to a unique group of eukaryotic methylotrophs that can use methanol as the sole source of carbon and energy. On the other hand, K. phaffii seems to be more characteristic of the common ancient yeast ancestors than the rapidly evolving S. cerevisiae. Comparative studies between S. cerevisiae and K. phaffii will shed light on the mechanisms of evolution of metabolic pathways and regulatory systems. Such studies are accelerated by the practical importance of K. phaffii as a common microbial production host in biotechnology.
In our studies, we demonstrate that some amino acids greatly affect gene expression in K. phaffii [1]. Transcriptome analysis revealed drastic changes in gene expression when proline was present in the media. About 18.9% of total protein-coding genes were differentially expressed, including genes involved in methanol utilization [2]. Our results show that the unique methanol metabolism pathway is regulated not only by methanol, but also by other carbon sources. Therefore, our findings suggest that the regulation of methanol metabolism pathway is integrated into other cellular regulatory networks. Methanol metabolism, acquired by K. phaffii during evolution, is tightly associated with nitrogen an amino acid metabolism.