bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel insight into the role of clusterin on intraocular pressure regulation by modifying actin polymerization and extracellular matrix remodeling in the trabecular meshwork
Authors: Soundararajan Avinash , Wang Ting , Ghag Sachin A. , Kang Min H. , Pattabiraman Padmanabhan P. 
Published: May 2022

AbstractThis study provides comprehensive mechanistic evidence for the role of clusterin, a stress‐response secretory chaperone protein, in the modulation of intraocular pressure (IOP) by regulating the trabecular meshwork (TM) actin cytoskeleton and the extracellular matrix (ECM). The pathological stressors on TM known to elevate IOP significantly lowered clusterin protein levels indicating stress‐related clusterin function loss. Small interfering RNA‐mediated clusterin loss in human TM cells in vitro induced actin polymerization and stabilization via protein kinase D1, serine/threonine‐protein kinase N2 (PRK2), and LIM kinase 1 (LIMK1), and the recruitment and activation of adhesome proteins including paxillin, vinculin, and integrin αV and β5. A complete loss of clusterin as seen in clusterin knockout mice (Clu‐/‐) led to significant IOP elevation at postnatal Day 70. Contrarily, constitutive clusterin expression using adenovirus (AdCLU) in HTM cells resulted in the loss of actin polymerization via decreased PRK2, and LIMK1 and negative regulation of integrin αV and β5. Furthermore, we found that AdCLU treatment in HTM cells significantly decreased the ECM protein expression and distribution by significantly increasing matrix metalloprotease 2 (MMP2) activity and lowering the levels of pro‐fibrotic proteins such as transforming growth factor‐β2 (TGFβ2), thrombospondin‐1 (TSP‐1), and plasminogen activator inhibitor‐1 (PAI‐1). Finally, we found that HTM cells supplemented with recombinant human clusterin attenuated the pro‐fibrotic effects of TGFβ2. For the first time this study demonstrates the importance of clusterin in the regulation of TM actin cytoskeleton ‐ ECM interactions and the maintenance of IOP, thus making clusterin an interesting target to reverse elevated IOP.
Recycling nutrients in the beef supply chain through circular manuresheds: Data to assess tradeoffs
Authors: Spiegal S. , Vendramini J. M. B. , Bittman S. , Silveira M. L. , Gifford C. , Rotz C. A. , Ragosta J. P. , Kleinman P. J. A. 
Published: May 2022

AbstractNutrient circularity can help supply chain participants meet sustainability targets. Across the segmented beef supply chain, opportunity exists to reinforce and introduce nutrient circularity by recycling surplus manure nutrients from cattle feedlots to lands where cattle feed is produced. We describe four datasets developed to evaluate options in U.S. and Canadian beef systems. The datasets delineate three “circular manuresheds,” each encompassing a hay‐grazing landscape where beef cattle are raised on grazingland and supplemented with hay grown nearby, and the distant feedlots where those cattle produce manure nutrients for potential import back to the hayfields. We selected the hay‐grazing landscapes of New Mexico, USA; Florida, USA; and western Canada (Manitoba, Saskatchewan, Alberta, British Columbia) because of their significant grazingland production and potential to substitute feedlot manure for commercial fertilizer on hayfields. In each circular manureshed, the manure nutrients from major feedlot destinations could supply a considerable proportion of the P used by hay for grazing cattle: 34% of the P requirements in New Mexico, 36% in Florida, and 6% in western Canada. The average distance to return the resource was 647 km for New Mexico, 1,884 km for Florida, and 1,587 km for western Canada. These magnitudes and distances suggest that the New Mexico circular manureshed may be the most economically viable in the current agri‐food system, but this reflects only part of a greater, multi‐factor assessment of tradeoffs. The circular manureshed concept provides a platform for simultaneous consideration of competing factors for sustainability via circularity.
Esophageal epithelial immunoglobulin G is an important marker for the diagnosis and management of pediatric eosinophilic esophagitis
Authors: Rizvi Syed Ahsan , Oriala Chukwuemeka , Irastorza Laura E , Bornstein Jeffrey , Li Shuan , Smadi Yamen 
Published: May 2022

AbstractBackground and AimOur primary aim was to describe the prevalence of immunoglobulin G (IgG) and its subclass IgG4 in immunohistochemistry staining in esophageal biopsy specimens of children with eosinophilic esophagitis (EoE) compared with that of specimens from children with gastroesophageal reflux disease (GERD).MethodsEsophageal biopsy specimens from children with EoE or GERD were stained prospectively for IgG and IgG4 antibodies. Subjects with EoE were divided into cohorts with either active EoE or EoE in remission. Active EoE cases were further divided into proton pump inhibitor responsive (PPI‐r) and PPI‐nonresponsive (PPI‐nr) subgroups. Demographic, clinical, and histologic data were compared among groups, including quantified IgG and IgG4 staining, peak eosinophil count, eosinophil‐derived neurotoxin levels, and EoE endoscopic reference score.ResultsSeventy‐nine children (aged 10.6 ± 5.6 years; 68% male) were enrolled. IgG‐positive cell counts were significantly elevated in those with active EoE (n = 29, 3 [interquartile range, IQR: 2–6]/high‐powered field [HPF]), compared with those having EoE remission (n = 25, 1 [IQR: 0–2]/HPF; P = 0.002) and those with GERD (n = 25, 0 [IQR: 0–0.25]/HPF, P = <0.0001). IgG‐positive cell counts were significantly higher in the PPI‐r (n = 15, 5 [IQR: 2.5–11]/HPF) subgroup, compared with the PPI‐nr subgroup (n = 11, 3 [IQR: 1.5–3.5]/HPF; P = 0.041) at baseline endoscopy.ConclusionInitial esophageal tissue biopsy specimens from pediatric subjects with active EoE showed a significant increase in IgG‐positive staining compared with tissue from subjects in EoE remission or with GERD. There was higher positivity of IgG‐stained cells in the PPI‐r subgroup compared with the PPI‐nr subgroup.
Expansion of granulocytic myeloid‐derived suppressor cells in patients with severe fever with thrombocytopenia syndrome
Authors: Du Juan , Li Xiao‐Kun , Peng Xue‐Fang , Xu Wen , Zhang Xiao‐Ai , Li Hao , Yang Tong , Yuan Chun , Chen Wei‐Wei , Li Chang , Lu Qing‐Bin , Liu Wei 
Published: May 2022

AbstractSevere fever with thrombocytopenia syndrome (SFTS), caused by novel bunyavirus (SFTSV), is a hemorrhagic fever with a high mortality rate of over 10%. We have previously shown that granulocytic myeloid‐derived suppressor cell (gMDSC) might affect arginine metabolism, which was associated with decreased platelet count and T lymphocyte dysfunction in this disease. The study was designed to investigate the expression of the gMDSCs subsets in SFTS patients, and to evaluate its association with disease severity. A prospective study was performed on 166 confirmed SFTSV infected patients. Sequential blood samples were collected during hospitalization and after recovery. SFTSV RNA was quantified by real‐time RT‐PCR. The gMDSCs and NK cells were determined by flow cytometry analysis, which were associated with disease severity. Elevation of the activated gMDSC was observed in SFTS patients at the acute phase, with a significantly higher level of gMDSC attained in 79 severe and 29 fatal SFTS patients than in the mild patients. The NK cells were depleted at the early infection and not restored to normal level until 4 months after the disease. The expansion of gMDSC was accompanied by the elevated expressions of CD3‐ζ of NK and Arginase‐1, in contrast with the decreased reactive oxygen species (ROS) in gMDSC. The levels of NK, CD3‐ζ of NK, viral load, and platelet count were significantly associated with the level of gMDSC. Expansion of gMDSC was demonstrated in SFTS, which was associated with severe disease and suppressed antiviral NK cell via other mechanisms than Arginase‐1 or ROS.
Infrared multiple‐angle incidence resolution spectrometry for vapor‐deposited amorphous water
Authors: Nagasawa Takumi , Numadate Naoki , Hama Tetsuya 
Published: May 2022

AbstractInfrared multiple‐angle incidence resolution spectrometry (IR‐MAIRS) is a recently developed spectroscopic technique that combines oblique incidence transmission measurements and chemometrics (multivariate analysis) to obtain both pure in‐plane (IP) and out‐of‐plane (OP) vibration spectra for a thin sample. IR‐MAIRS is established for analyzing the molecular orientation of organic thin films at atmospheric pressure, but it should also be powerful for the structural characterization of vapor‐deposited thin samples prepared in a vacuum. The application of IR‐MAIRS to vapor‐deposited amorphous water is particularly interesting in the fields of physical and interstellar chemistry, because it is a representative model material for interstellar icy dust grains. We recently developed an experimental setup for in situ IR‐MAIRS under low‐temperature, ultrahigh‐vacuum conditions, which thus facilitates measurements of interstellar ice analogs such as vapor‐deposited amorphous water. This review considers the theoretical framework of IR‐MAIRS and our recent experimental results for vapor‐deposited amorphous water. We present spectroscopic signatures for the perpendicular orientation of dangling‐OH bonds for three‐coordinated water molecules at the surface of amorphous water at 90 K. The absolute absorption cross section of the three‐coordinated dangling‐OH bonds is quantitatively measured as 1.0 ± 0.2 × 10−18 cm2 at 3696 cm−1. As IR‐MAIRS can essentially be conducted using only a Fourier‐transform IR spectrometer and an angle‐controllable linear polarizer, it is a useful, low‐cost, and simple spectroscopic technique for studying laboratory analogs of interstellar ices including vapor‐deposited amorphous water.
Crystal structure, phase transitions and Raman scattering in the new non‐centrosymmetric [(C3H7)4N]3Bi3Br12 compound
Authors: Trigui Wala , Lhoste Jerome , Auguste Sandy , Oueslati Abderrazek 
Published: May 2022

AbstractA novel tri‐tetrapropylammonium dodeca bromobismuthate (III) compound has been synthesized and studied by single‐crystal X‐ray diffraction, differential scanning calorimetry, and Raman spectroscopy as a function of temperature. At room temperature, the title compound crystalizes in noncentrosymmetric Cc space group which belongs to monoclinic crystal system. The unit cell dimensions are a = 11.083(6) Å, b = 19.100(6) Å, c = 31.146(3) Å, β = 98.42(0)°, and V = 6522.69(9) Å3. The crystal package is provided by electrostatic interactions and hydrogen bonds (C–H … Br). Intermolecular interactions present in the grown single crystal were analyzed by Hirshfeld surface and 2D fingerprint plot. The differential scanning calorimetry reveals two reversible phase transitions at 297 and 430 K. The values of the transition entropies suggest that the first is of an order–disorder type, while the second appears as a displacive one. The Raman versus temperature studies show that the vibrational states of tetrapropylammonium cations change markedly through the phase transitions. The most important changes are observed for two lines at 1034 and 1449 cm−1 (at room temperature) issued from the νs(N–C–C) + δs(C–N–C) and δs (CH3) of the cations. The spectral characteristics of these lines are analyzed and consistently described in the framework of an order–disorder model for the phase transitions.
Comparative assessment of antioxidant and immunomodulatory properties of skimmed milk protein hydrolysates and their incorporation in beverage mix
Authors: Prakash Pavan Kumar , Eligar Sachin M , Prakruthi Manikumar , Jyothi Lakshmi Aswathanarayana setty 
Published: May 2022

AbstractBACKGROUNDMilk‐derived protein hydrolysates have generated a great deal of interest recently due to their numerous beneficial health effects. However, there are few comparative studies on protein hydrolysates from different dairy species, their production, characterization, and bioactivity. In the present study, skimmed milk from both major and minor dairy species was hydrolyzed with alcalase, and its protein profiles were studied using tricine polyacrylamide gel electrophoresis and reverse phase‐high protein liquid chromatography. The antioxidant and in vitro immunostimulatory properties were determined.RESULTSIron chelation activity was highest in hydrolysates of whey (25.00 ± 0.32 mmol L−1), casein (25.14 ± 0.34 mmol L−1), colostrum (24.52 ± 0.28 mmol L−1), and skimmed cattle milk (24.21 ± 0.26 mmol L−1 ). α,α‐Diphenyl‐β‐picrylhydrazyl scavenging and 2,2′‐azobis(2‐amidino‐propane) dihydrochloride activity was lowest in skimmed donkey milk protein hydrolysates (MPHs) (IC50: 5.37 ± 0.05 mg mL−1 and 151.59 ± 2.1 mg mL−1). Production of nitric oxide and phagocytosis activity in RAW 264.7 (murine macrophage cell line) was significantly higher among whey and buffalo skimmed milk protein hydrolysate‐treated groups as compared with the untreated group. The incorporation of whey protein hydrolysate and skimmed buffalo milk protein hydrolysate were sensorially acceptable at 10% level in beverage mix.CONCLUSIONThis study comparatively evaluates the antioxidative and immunomodulatory properties of different skimmed MPHs and their potential applications as ingredients in pediatric, geriatric, and other health‐promoting foods. © 2022 Society of Chemical Industry.
Recent application of artificial neural network in microwave drying of foods: a mini‐review
Authors: Yang Ran , Chen Jiajia 
Published: May 2022

AbstractThe microwave‐assisted thermal process is a high‐efficiency drying method and is promising to be applied in the food industry. However, the prediction of the thermal treatment results from such a dynamic and complicated process can be difficult. Additionally, the determination of the optimal drying parameters, such as drying temperature, microwave power, and drying time for optimized performance can also be hard. Recently, extensive research has been focusing on the use of artificial neural network (ANN) models in the laboratory‐scale microwave drying processes and has shown the feasibility of such application. As a regression tool, the ANN models have been widely used in predicting drying performance; when integrated with additional optimizing algorithms, the ANN models could be used for drying parameter optimization; and when combined with real‐time measuring techniques (e.g. nuclear magnetic resonance), the ANN models could be used for monitoring and controlling the drying process in a dynamic sense. Future research could focus on testing the developed ANN models in industrial‐scale microwave drying processes and applying the ANN models in microwave drying kinetics research for optimizing the dynamic drying processes. © 2022 Society of Chemical Industry.
Agroclimatic zoning for bananas under climate change in Brazil
Authors: de Olanda Souza Gabriel Henrique , de Oliveira Aparecido Lucas Eduardo , de Lima Rafael Fausto , Torsoni Guilherme Botega , Chiquitto Alisson Gaspar , de Moraes Jose Reinaldo Cabral 
Published: May 2022

AbstractBACKGROUNDClimate change is the main cause of biotic and abiotic stresses in plants and affects yield. Therefore, we sought to carry out a study on future changes in the agroclimatic conditions of banana cultivation in Brazil. The current agroclimatic zoning was carried out with data obtained from the National Institute of Meteorology related to mean air temperature, annual rainfall, and soil texture data in Brazil. The global climate model BCC‐CSM1.1 (Beijing Climate Center‐Climate System Model, version 1.1), adopted by the Intergovernmental Panel on Climate Change, corresponding to Representative Concentration Pathways (RCPs) 2.6, 4.5, 6.0, and 8.5 for the period 2050 (2041–2060) and 2070 (2061–2080), obtained through the CHELSA V1.2 platform, was chosen for the climate projections of the Coupled Model Intercomparison Project 5. Matrix images at a depth of 5–15 cm, obtained through the product of the SoilGrids system, were used for the texture data. ArcGIS version 10.8 was used to construct the maps.RESULTSAreas favorable to the crop plantation were classified as suitable when air temperatureTAIRwas between 20 and 29 °C, annual rainfallRANNUALbetween 1200 and 1900 mm, and soil clay contentCSOILbetween 30 and 55%. Subsequently, the information was reclassified, summarizing the classes into preferential, recommended, little recommended, and not recommended. The current scenario shows a preferential class of 8.1%, recommended of 44.6%, little recommended of 47.1%, and not recommended of 0.1% for the Brazilian territory.CONCLUSIONThe results show no drastic changes in the total area regarding the classes, but there is a migration from these zones; that is, from tropical to subtropical and temperate regions. RCP 8.5–2070 (2061–2080) showed trends with negative impacts on arable areas for banana cultivation at the end of the century. © 2022 Society of Chemical Industry.
Kinetic degradation study for the first licensed anti‐influenza polymerase inhibitor, baloxavir marboxil, using high‐performance liquid chromatography‐mass spectrometry
Authors: Hafez Hani M. , Abdel‐Halim Mohammad , Hemdan Ahmed , Hammam Mennatallah A. 
Published: May 2022

The first licensed polymerase inhibitor, baloxavir marboxil was recently approved for the treatment of influenza A and B viruses. Furthermore, there is growing interest in testing the antiviral activity of baloxavir marboxil against Coronavirus. Despite its critical clinical value, there is no information on the degradation products, pathways, or kinetics of baloxavir marboxil under various stress conditions. In this study, a new high‐performance liquid chromatography‐ultraviolet detection method for accurately quantifying baloxavir marboxil in the presence of its degradation products was developed. A study of degradation kinetics revealed that acidic, thermal neutral, and photolytic degradation reactions have zero‐order kinetics, whereas basic and oxidative degradation reactions have first‐order kinetics. The structural characterization of baloxavir marboxil degradation products was performed by coupling the optimized high‐performance liquid chromatography method to the triple‐quadrupole tandem mass spectrometer. The proposed approach was validated according to the International Council for Harmonisation Q2 (R1) requirements for accuracy, precision, robustness, specificity, and linearity. The validated new method was successfully used to analyze baloxavir marboxil as raw material and its pharmaceutical dosage form, Xofluza.
Sociodemographic Factors and Health Care Utilization in Pediatric Chronic Suppurative Otitis Media
Authors: Ruffin William C. , Crouch Jacob B. , Burruss Clayton P. , Bush Matthew L. 
Published: May 2022

ObjectivesAccess to and utilization of perioperative healthcare may influence outcomes in patients with chronic suppurative otitis media (CSOM); however, the influencing factors are poorly understood. The objective of this study was to assess the association of sociodemographic factors with perioperative health care utilization for pediatric CSOM patients.MethodsWe conducted a review on pediatric CSOM patients (≤18 years old) who underwent tympanoplasty with/without mastoidectomy between 2010 and 2020. Sociodemographic data and clinical were collected on all participants. Univariate and multivariate logistic regression analysis was conducted to assess the association between these factors and perioperative care utilization.Results427 patients were included in the study. The primary factor associated with health care utilization was insurance status. Rural children were more likely to have Medicaid insurance (p = 0.048). For tympanoplasty patients, Medicaid patients have 1.66 higher odds of pre‐operative no‐shows (p = 0.01), 1.31 higher odds of post‐operative no‐shows (p = 0.02), and 59% lower odds of having a postoperative audiogram (p = 0.01), and 2.64 higher odds of being from a rural community (p = 0.02). For patients undergoing mastoidectomy, Medicaid patients have 1.25 higher odds of postoperative no‐shows (p = 0.01), 39% lower odds of having a revision procedure for ossicular reconstruction (p = 0.045). Appalachian rural mastoidectomy patients had 3.62 higher odds of having cholesteatoma (p = 0.05).ConclusionPediatric patients with CSOM who have Medicaid insurance, especially those who reside in rural regions, are at risk for lower perioperative hearing health care utilization. As these findings may impact care delivery and clinical outcomes, efforts should be focused on promoting utilization among these populations.Level of Evidence4 Laryngoscope, 133:700–705, 2023
Role of HER2 in Prognosis of Salivary Duct Carcinoma: A Systematic Review and Meta‐Analysis
Authors: Williams Christopher Y.K. , Townson Adam T. , Terry Nancy , Schmitt Nicole C. , Sharma Arun 
Published: May 2022

ObjectivesSalivary duct carcinoma (SDC) is a rare, aggressive malignancy with a poor prognosis. These tumors frequently stain positive for HER2/ErbB2, but data on the prognostic significance of HER2 status in SDC are mixed. We sought to determine whether HER2 status affects survival outcomes in SDC.MethodsPubMed, Embase, and Web of Science databases were searched from inception to October 2020. Eligibility was restricted to studies reporting HER2/ErbB2 overexpression in histologically confirmed de novo SDC or SDC ex pleomorphic adenoma, with corresponding overall (OS) and disease‐free (DFS) survival measures. Separate multivariable and univariable meta‐analyses were performed using random‐effects models. Statistical heterogeneity was estimated by Cochran's Q and I2 tests. Funnel plots were generated and Egger's test was used to assess for publication bias. The risk of bias was assessed with the Newcastle‐Ottawa Scale.ResultsOf 183 unique citations, 14 studies of 663 patients were included. Most included studies determined HER2 status according to ASCO/CAP guidelines. The univariable meta‐analysis did not reveal an effect between HER2 status and OS (HR 1.09, 95% CI 0.84–1.42). In the multivariable analysis, HER2 positivity was associated with a HR of 1.49 for OS (95% CI 0.96–2.30). Fewer studies reported data for DFS than OS, with no relationship between HER2 status and DFS found on multivariable or univariable meta‐analyses.ConclusionIn patients with salivary duct carcinoma, HER2 positivity was not found to be associated with worse overall survival. This information may be useful when counseling patients and considering treatment options. Laryngoscope, 133:476–484, 2023