bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longevity of Trichuris trichiura infection in the human host
Authors: Akelew Yibeltal , Whitehead Bradley , Nejsum Peter 
Published: August 2022

In this research, we present evidence-based data for the longevity of Trichuris trichiura on a healthy volunteer who had ingested eggs of T. trichiura. Hence, T. trichiura lives in a human host for at least 6 years and 5 months. Concurrently, this study highlighted consideration for returning travellers and migrants.
Tissue-specific mRNA profiling of theBrassica napus–Sclerotinia sclerotioruminteraction uncovers novel regulators of plant immunity
Authors: Walker Philip L , Girard Ian J , Becker Michael G , Giesbrecht Shayna , Whyard Steve , Fernando W G Dilantha , de Kievit Teresa R , Belmonte Mark F 
Published: August 2022

AbstractWhite mold is caused by the fungal pathogen Sclerotinia sclerotiorum and leads to rapid and significant loss in plant yield. Among its many brassicaceous hosts, including Brassica napus (canola) and Arabidopsis, the response of individual tissue layers directly at the site of infection has yet to be explored. Using laser microdissection coupled with RNA sequencing, we profiled the epidermis, mesophyll, and vascular leaf tissue layers of B. napus in response to S. sclerotiorum. High-throughput tissue-specific mRNA sequencing increased the total number of detected transcripts compared with whole-leaf assessments and provided novel insight into the conserved and specific roles of ontogenetically distinct leaf tissue layers in response to infection. When subjected to pathogen infection, the epidermis, mesophyll, and vasculature activate both specific and shared gene sets. Putative defense genes identified through transcription factor network analysis were then screened for susceptibility against necrotrophic, hemi-biotrophic, and biotrophic pathogens. Arabidopsis deficient in PR5-like RECEPTOR KINASE (PR5K) mRNA levels were universally susceptible to all pathogens tested and were further characterized to identify putative interacting partners involved in the PR5K signaling pathway. Together, these data provide insight into the complexity of the plant defense response directly at the site of infection.
Essential Thrombocythemia and Post-Essential Thrombocythemia Myelofibrosis: Updates on Diagnosis, Clinical Aspects, and Management
Authors: Puglianini Omar Castaneda , Peker Deniz , Zhang Linsheng , Papadantonakis Nikolaos 
Published: August 2022

AbstractAlthough several decades have passed since the description of myeloproliferative neoplasms (MPN), many aspects of their pathophysiology have not been elucidated. In this review, we discuss the mutational landscape of patients with essential thrombocythemia (ET), prognostic scores and salient pathology, and clinical points. We discuss also the diagnostic challenges of differentiating ET from prefibrotic MF.We then focus on post-essential thrombocythemia myelofibrosis (post-ET MF), a rare subset of MPN that is usually studied in conjunction with post-polycythemia vera MF. The transition of ET to post-ET MF is not well studied on a molecular level, and we present available data. Patients with secondary MF could benefit from allogenic hematopoietic stem cell transplantation, and we present available data focusing on post-ET MF.
GECEM Project Database: A digital humanities solution to analyse complex historical realities in early modern China and Europe
Authors: Perez-Garcia Manuel , Diaz-Ordoñez Manuel 
Published: August 2022

AbstractThe GECEM Project Database stands out as a new Digital Humanities solution to accurately order and analyse the new historical Big Data gathered in Chinese and European historical archives. Traditional challenges such as capture, storage, analysis, data curation, searching, sharing, transfer, visualization, querying, updating, and information privacy are being tackled and solved within the design of this new multi-relational database. The implementation of this database has as its main innovative elements the capability of coding and cross-referring historical data in Chinese and Western languages. Thus, we present a new database as a digital solution to solve the long-lasting problem in historical research: the maximization and optimization of data collection when analysing historical sources and how to make use of a large amount of registers and information when developing case studies and work hypotheses for historical research. In this paper, we present a solution for data collection and analysis of probate inventories and trade records which is a relevant source when determining changes in patterns of consumption and global trade through the introduction of Chinese goods into Europe and vice versa during the early modern period.
Exposure Therapy for Child and Adolescent Anxiety and OCD
Authors: Whiteside Stephen P. , Ollendick Thomas H. , Biggs Bridget K. 
Published: August 2022

Abstract
Exposure is the most important component of therapy for anxiety disorders and obsessive–compulsive disorder (OCD) in children and adolescents. Unfortunately, few clinicians offer this treatment, making it very difficult for youth and their families to seek effective care. In this installment of the Association for Behavioral and Cognitive Therapy’s Series on Implementation of Clinical Approaches, Whiteside, Ollendick, and Biggs deliver a succinct yet comprehensive guide to the use of exposure therapy with youth suffering from anxiety and OCD. Within the heart of this book, clinicians will discover a clear step-by-step model, illustrated with sample dialogue, for engaging their young patients in this most effective treatment. Detailed case examples bring to life the application of all forms of exposure (in vivo, imaginal, and interoceptive) to a wide range of anxiety and OCD presentations. Beyond teaching the mechanics for implementing exposure, the authors present a clinical model for understanding how exposure works, synthesizing the key issues from current competing theories. This model can enhance the ability of clinicians to apply exposure to new and more challenging presentations. In addition, the authors review common challenges to implementing exposure in real-world settings to assist clinicians in overcoming frequently encountered barriers. Clinicians can feel confident in the validity of the approach as the authors concisely review the foundation of history and research supporting exposure. Within this compact book, clinicians will find a comprehensive guide to build their expertise in the delivery of exposure therapy for children and adolescents suffering from anxiety disorders or OCD.
A census of post-AGB stars in Gaia DR3: evidence for a substantial population of Galactic post-RGB stars
Authors: Oudmaijer René D , Jones Emma R M , Vioque Miguel 
Published: August 2022

ABSTRACT
This paper presents the first census of Galactic post-asymptotic giant branch (post-AGB) stars in the Hertzsprung–Russell (HR) diagram. We combined Gaia DR3 parallax-based distances with extinction corrected integrated fluxes and derived luminosities for a sample of 185 stars that had been proposed to be post-AGB stars in the literature. The luminosities allow us to create an HR diagram containing the largest number of post-AGB candidate objects to date. A significant fraction of the objects fall outside the typical luminosity range as covered by theoretical evolutionary post-AGB tracks as well as observed for planetary nebula central stars. These include massive evolved supergiants and lower luminosity objects. Here, we highlight the fact that one-third of the post-AGB candidates are underluminous and we identify these with the recently recognized class of post-red giant branch objects thought to be the result of binary evolution.
Alteration of RNA modification signature in human sperm correlates with sperm motility
Authors: Guo Huanping , Shen Xipeng , Hu Hua , Zhou Peng , He Tong , Xia Lin , Tan Dongmei , Zhang Xi , Zhang Yunfang 
Published: August 2022

Abstract
RNA modifications, which are introduced post-transcriptionally, have recently been assigned pivotal roles in the regulation of spermatogenesis and embryonic development. However, the RNA modification landscape in human sperm is poorly characterized, hampering our understanding about the potential role played by RNA modification in sperm. Through our recently developed high-throughput RNA modification detection platform based on liquid chromatography with tandem mass spectroscopy, we are the first to have characterized the RNA modification signature in human sperm. The RNA modification signature was generated on the basis of 49 samples from participants, including 13 healthy controls, 21 patients with asthenozoospermia (AZS) and 15 patients with teratozoospermia (TZS). In total, we identified 13 types of RNA modification marks on the total RNA in sperm, and 16 types of RNA modification marks on sperm RNA fragments of different sizes. The levels of these RNA modifications on the RNA of patients with AZS or TZS were altered, compared to controls, especially on sperm RNA fragments &gt;80 nt. A few types of RNA modifications, such as m1G, m5C, m2G and m1A, showed clear co-expression patterns as well as high linear correlations with clinical sperm motility. In conclusion, we characterized the RNA modification signature of human sperm and identified its correlation with sperm motility, providing promising candidates for use in clinical sperm quality assessment and new research insights for exploring the underlying pathological mechanisms in human male infertility syndromes.
Alternative dietary protein sources to support healthy and active skeletal muscle aging
Authors: van der Heijden Ino , Monteyne Alistair J , Stephens Francis B , Wall Benjamin T 
Published: August 2022

AbstractTo mitigate the age-related decline in skeletal muscle quantity and quality, and the associated negative health outcomes, it has been proposed that dietary protein recommendations for older adults should be increased alongside an active lifestyle and/or structured exercise training. Concomitantly, there are growing environmental concerns associated with the production of animal-based dietary protein sources. The question therefore arises as to where this dietary protein required for meeting the protein demands of the rapidly aging global population should (or could) be obtained. Various non-animal–derived protein sources possess favorable sustainability credentials, though much less is known (compared with animal-derived proteins) about their ability to influence muscle anabolism. It is also likely that the anabolic potential of various alternative protein sources varies markedly, with the majority of options remaining to be investigated. The purpose of this review was to thoroughly assess the current evidence base for the utility of alternative protein sources (plants, fungi, insects, algae, and lab-grown “meat”) to support muscle anabolism in (active) older adults. The solid existing data portfolio requires considerable expansion to encompass the strategic evaluation of the various types of dietary protein sources. Such data will ultimately be necessary to support desirable alterations and refinements in nutritional guidelines to support healthy and active aging, while concomitantly securing a sustainable food future.
Intertemporal planning with subjective uncertainty: anticipating your lazy, disorganized self
Authors: Chatterjee Kalyan , Krishna R Vijay , Sopher Barry 
Published: August 2022

Abstract
We investigate intertemporal planning problems as a way of gaining understanding of the characteristics of individual decision-makers and the choice options presented to them. A frequent simplifying assumption that is made in studies of this sort is that choice of options that yield lower monetary payments than other available options is suboptimal, but consideration of subjective uncertainty in fulfilling requirements to obtain future payments easily disposes of this notion. For example, if one chooses an option in which one pays zero interest for a year on a purchase but then fails to pay the item off before high interest charges kick in, this would be considered suboptimal, compared with paying the item off up front, or in some other fashion. The important point is that what makes an action optimal or suboptimal is often contingent on information that is essentially unobservable, specifically, the probability that one will fail to pay the item off in time. In the experiment, we make inferences about subjective uncertainty based on the choices one makes.
Mathematical Progress — On Maddy and Beyond
Authors: Weisgerber Simon 
Published: August 2022

AbstractA key question of the ‘maverick’ tradition of the philosophy of mathematical practice is addressed, namely what is mathematical progress. The investigation is based on an article by Penelope Maddy devoted to this topic in which she considers only contributions ‘of some mathematical importance’ as progress. With the help of a case study from contemporary mathematics, more precisely from tropical geometry, a few issues with her proposal are identified. Taking these issues into consideration, an alternative account of ‘mathematical importance’, broadly within the framework of progress Maddy offers, is developed with a special focus on mathematicians’ peer-review practice.
A gain-of-function allele of a DREB transcription factor gene ameliorates drought tolerance in wheat
Authors: Mei Fangming , Chen Bin , Du Linying , Li Shumin , Zhu Dehe , Chen Nan , Zhang Yifang , Li Fangfang , Wang Zhongxue , Cheng Xinxiu , Ding Li , Kang Zhensheng , Mao Hude 
Published: August 2022

AbstractDrought is a major environmental factor limiting wheat production worldwide. However, the genetic components underlying wheat drought tolerance are largely unknown. Here, we identify a DREB transcription factor gene (TaDTG6-B) by genome-wide association study that is tightly associated with drought tolerance in wheat. Candidate gene association analysis revealed that a 26-bp deletion in the TaDTG6-B coding region induces a gain-of-function for TaDTG6-BDel574, which exhibits stronger transcriptional activation, protein interactions, and binding activity to dehydration-responsive elements (DRE)/CRT cis-elements than the TaDTG6-BIn574 encoded by the allele lacking the deletion, thus conferring greater drought tolerance in wheat seedlings harboring this variant. Knockdown of TaDTG6-BDel574 transcripts attenuated drought tolerance in transgenic wheat, whereas its overexpression resulted in enhanced drought tolerance without accompanying phenotypic abnormalities. Furthermore, the introgression of the TaDTG6-BDel574 elite allele into drought-sensitive cultivars improved their drought tolerance, thus providing a valuable genetic resource for wheat breeding. We also identified 268 putative target genes that are directly bound and transcriptionally regulated by TaDTG6-BDel574. Further analysis showed that TaDTG6-BDel574 positively regulates TaPIF1 transcription to enhance wheat drought tolerance. These results describe the genetic basis and accompanying mechanism driving phenotypic variation in wheat drought tolerance, and provide a novel genetic resource for crop breeding programs.
Type one protein phosphatase regulates fixed-carbon starvation-induced autophagy in Arabidopsis
Authors: Wang Qiuling , Qin Qianqian , Su Meifei , Li Na , Zhang Jing , Liu Yang , Yan Longfeng , Hou Suiwen 
Published: August 2022

Abstract
Autophagy, a conserved pathway that carries out the bulk degradation of cytoplasmic material in eukaryotic cells, is critical for plant physiology and development. This process is tightly regulated by ATG13, a core component of the ATG1 kinase complex, which initiates autophagy. Although ATG13 is known to be dephosphorylated immediately after nutrient starvation, the phosphatase regulating this process is poorly understood. Here, we determined that the Arabidopsis (Arabidopsis thaliana) septuple mutant (topp-7m) and octuple mutant (topp-8m) of TYPE ONE PROTEIN PHOSPHATASE (TOPP) exhibited significantly reduced tolerance to fixed-carbon (C) starvation due to compromised autophagy activity. Genetic analysis placed TOPP upstream of autophagy. Interestingly, ATG13a was found to be an interactor of TOPP. TOPP directly dephosphorylated ATG13a in vitro and in vivo. We identified 18 phosphorylation sites in ATG13a by LC-MS. Phospho-dead ATG13a at these 18 sites significantly promoted autophagy and increased the tolerance of the atg13ab mutant to fixed-C starvation. The dephosphorylation of ATG13a facilitated ATG1a-ATG13a complex formation. Consistently, the recruitment of ATG13a for ATG1a was markedly inhibited in topp-7m-1. Finally, TOPP-controlled dephosphorylation of ATG13a boosted ATG1a phosphorylation. Taken together, our study reveals the crucial role of TOPP in regulating autophagy by stimulating the formation of the ATG1a-ATG13a complex by dephosphorylating ATG13a in Arabidopsis.
Antiphospholipid antibodies in patients with calcific aortic valve stenosis
Authors: Plunde Oscar , Svenungsson Elisabet , Ferrannini Giulia , Franco-Cereceda Anders , Bäck Magnus 
Published: August 2022

AbstractObjectivesThe antiphospholipid syndrome is defined by antiphospholipid antibodies (aPL) together with arterial and/or venous thromboembolism and/or obstetric morbidities. aPL are overrepresented in SLE and acute myocardial infarction, but it is unknown whether aPL are associated with calcific aortic valve stenosis (CAVS) in the general population. The prevalence of aPL and other SLE-associated autoantibodies and their impact on aortic valve transcriptomics were therefore determined.MethodsA total of 233 tricuspid CAVS cases (median age 74, 69% male) and an age- and sex-matched control population were included. aPL were measured as anti-cardiolipin and anti-β2Glycoprotein-I of IgG/M/A isotypes. Resilient, thickened and calcified aortic valve (AV) tissue derived from five aPL positive and five matched aPL negative CAVS patients undergoing surgical aortic valve replacement were analysed by microarrays.ResultsThe prevalence of positivity for any aPL (IgG/M/A) in patients with CAVS was 6.4% (95% CI 3.6% – 10.4%: n = 233). aPL IgG was significantly more prevalent in CAVS cases vs controls (4.6% vs 0.6%, P = 0.04). AV tissue from aPL IgG/IgM-positive patients was negatively enriched in pathways related to interferon signalling. One hundred differentially expressed genes could predict local AV CAVS progression with supervised machine learning algorithms.ConclusionsaPL IgG was more common in CAVS patients compared with matched controls and aPL positivity was associated with altered AV transcriptomics related to local disease progression and interferon pathways. Further studies should aim to establish aPL as a possible risk marker and/or causal factor for CAVS and could offer new precision therapeutic targets.
Method for reproducible automated bacterial cell culture and measurement
Authors: Ross David , Tonner Peter D , Vasilyeva Olga B 
Published: August 2022

Abstract
Microbial cell culture is one of the most commonly performed protocols for synthetic biology, and laboratories are increasingly using 96-well plates and laboratory automation systems for cell culture. Here, we describe a method for reproducible microbial culture using laboratory automation systems, including automated liquid handling, automated plate sealing and de-sealing, automated incubation and measurement of growing cultures. We discuss the key considerations that, in our experience, are important for reproducibility and present statistical analyses of data from 150 automated microbial growth experiments performed over 27 months using our automated method.
Androgen receptor splicing variant 7 (ARv7) promotes DNA damage response in prostate cancer cells
Authors: Luo Haoge , Liu Yanan , Li Yang , Zhang Chaoke , Yu Bingbing , Shao Chen 
Published: August 2022

AbstractIn the treatment of patients with locally advanced prostate cancer (PCa), androgen deprivation therapy (ADT) significantly enhances the efficacy of radiotherapy by weakening the DNA damage response (DDR) pathway. Recently, several studies have suggested that androgen receptor splicing variants (ARvs) may mediate a compensatory DDR pathway when canonical androgen receptor (AR) signaling is inhibited, thus contributing to the resistance of some patients to this combinational treatment. However, the specific roles of certain ARvs as well as the detailed mechanism of how ARvs regulate the DDR are not well understood. Here, we demonstrated that AR splicing variant 7 (ARv7), which is the most abundant form of ARvs, significantly promotes the DDR of PCa cells under severe DNA damage independent of its parental AR by using the ionizing radiation (IR) and doxorubicin (Dox)‐treated cell models. Mechanistically, ARv7 is sufficient to upregulate both the homologous recombination (HR) and the nonhomologous end joining (NHEJ) pathways by forming a positive regulatory loop with poly ADP‐ribose polymerase 1 (PARP1). Moreover, the presence of ARv7 impairs the synergistic effect between AR antagonists and poly ADP‐ribose polymerase (PARP) inhibitor, which has been recently shown to be a promising future treatment strategy for metastatic castration resistant prostate cancer (mCRPC). Combined, our data indicate that constitutively active ARv7 not only contributes to radioresistance after ADT, but may also serve as a potential predictive biomarker for assessing the efficacy of novel PARP inhibitor‐based therapy in PCa.
Interprofessional Education Innovation: Telehealth Objective Structured Clinical Examination With Family Nurse Practitioner and Pharmacy Students
Authors: Gilbert Bethany McNatt , Budisalich Kimberly , Morgan Tracie Clark 
Published: August 2022

Abstract
Interprofessional education (IPE) is required in the advanced nursing practice curriculum to promote collaboration between health care professionals. Time constraints, accessibility, and geographical locations are common barriers to overcome when considering implementing IPE experiences. Utilizing virtual modalities to develop these experiences can increase IPE opportunities. An innovative approach was taken to incorporate a telehealth Objective Structured Clinical Examination involving family nurse practitioner and pharmacy students from different academic institutions for an IPE virtual simulation. Faculty evaluated student performance based on competencies. Faculty and student feedback regarding the IPE experience was positive.
The effect of angiotensin receptor blockers and angiotensin-converting enzyme inhibitors on progression of gastric cancer: systematic review and meta-analysis
Authors: Mehrdad Seyed Ali , Mirzavi Farshad , Seyedi Seyed Mohammad Reza , Asoodeh Ahmad 
Published: August 2022

Angiotensin receptor blockers (ARB), as well as angiotensin-converting enzyme inhibitors (ACEI), are mostly used as therapy for hypertension and cardiovascular disease. However, they can increase the risk of cancer progression including gastric cancer. Here we aimed to analyze the assessment between ARB and ACEI on the progression of gastric cancer. Cochrane Library, PubMed and EMBASE were searched for articles and abstracts describing ARBs, ACEIs, and incidence of gastric cancer. Risk ratio, hazard ratio and 95% confidence interval (CI) were extracted from each outcome by using a random-effects model. Six studies met our inclusion criteria. These results demonstrated that there is a significant association between ARB with gastric cancer progression (risk ratio = 0.63; 95% CI, 0.5–0.7; P = 0.00; I

2
= 27.299; df (Q) = 2; Q-value = 2.75). However, there was not any link between ACEIs and gastric cancer development (risk ratio = 1.1; 95% CI, 0.92–1.31; P = 0.26; I

2
= 0.00; df (Q) = 3; Q-value = 1.26). All these findings indicated that using the ARBs has raised the progression of gastric cancer in these patients.