bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impaired SERCA2a phosphorylation causes diabetic cardiomyopathy through impinging on cardiac contractility and precursor protein processing
Authors: Quan Chao , Zhu Sangsang , Wang Ruizhen , Chen Jiamou , Chen Qiaoli , Li Min , Su Shu , Du Qian , Liu Minjun , Wang Hong-Yu , Chen Shuai 
Published: July 2022

Abstract
Diabetic cardiomyopathy (DCM) is currently a progressive and nonstoppable complication in type 2 diabetic patients. Metabolic insults and insulin resistance are involved in its pathogenesis; however, the underlying mechanisms are still not clearly understood. Here we show that calcium dysregulation can be both a cause and a consequence of cardiac insulin resistance that leads to DCM. A western diet induces the development of DCM through at least three phases in mice, among which an early phase depends on impaired Thr484-phosphorylation of sarcoplasmic/endoplasmic reticulum calcium ATPase 2a (SERCA2a) elicited by insulin resistance. Mutation of SERCA2a-Thr484 to a nonphosphorylatable alanine delays calcium re-uptake into the sarcoplasmic reticulum in the cardiomyocytes and decreases cardiac function at the baseline. Importantly, this mutation blunts the early phase of DCM, but has no effect on disease progression in the following phases. Interestingly, impairment of sarcoplasmic reticulum calcium re-uptake caused by the SERCA2a-Thr484 mutation inhibited processing of insulin receptor precursor through FURIN convertase, resulting in cardiac insulin resistance. Collectively, these data reveal a bidirectional relationship between insulin resistance and impairment of calcium homeostasis, which may underlie the early pathogenesis of DCM. Our findings have therapeutic implications for early intervention of DCM.
Four bright eclipsing binaries with γ Doradus pulsating components: CM Lac, MZ Lac, RX Dra, and V2077 Cyg
Authors: Southworth John , Van Reeth Timothy 
Published: July 2022

ABSTRACT
The study of pulsating stars in eclipsing binaries holds the promise of combining two different ways of measuring the physical properties of a star to obtain improved constraints on stellar theory. Gravity (g) mode pulsations such as those found in γ Doradus stars can be used to probe rotational profiles, mixing, and magnetic fields. Until recently few γ Doradus stars in eclipsing binaries were known. We have discovered g-mode pulsations in four detached eclipsing binary systems from light curves obtained by the Transiting Exoplanet Survey Satellite (TESS) and present an analysis of their eclipses and pulsational characteristics. We find unresolved g-mode pulsations at frequencies 1–1.5 d−1 in CM Lac, and measure the masses and radii of the component stars from the TESS data and published radial velocities. MZ Lac shows a much richer frequency spectrum, including pressure modes and tidally excited g-modes. RX Dra is in the northern continuous viewing zone of TESS so has a light-curve covering a full year, but shows relatively few pulsation frequencies. For V2077 Cyg we formally measure four pulsation frequencies, but the available data are inadequate to properly resolve the g-mode pulsations. V2077 Cyg also shows total eclipses, with which we obtain the first measurement of the surface gravity of the faint secondary star. All four systems are bright and good candidates for detailed study. Further TESS observations are scheduled for all four systems, with much improved temporal baselines in the cases of RX Dra and V2077 Cyg.
Cross-platform analysis reveals cellular and molecular landscape of glioblastoma invasion
Authors: Chen Ann T , Xiao Yang , Tang Xiangjun , Baqri Mehdi , Gao Xingchun , Reschke Melanie , Sheu Wendy C , Long Gretchen , Zhou Yu , Deng Gang , Zhang Shenqi , Deng Yanxiang , Bai Zhiliang , Kim Dongjoo , Huttner Anita , Kunes Russell , Günel Murat , Moliterno Jennifer , Saltzman W Mark , Fan Rong , Zhou Jiangbing 
Published: July 2022

AbstractBackgroundImproved treatment of glioblastoma (GBM) needs to address tumor invasion, a hallmark of the disease that remains poorly understood. In this study, we profiled GBM invasion through integrative analysis of histological and single-cell RNA sequencing (scRNA-seq) data from 10 patients.MethodsHuman histology samples, patient-derived xenograft mouse histology samples, and scRNA-seq data were collected from 10 GBM patients. Tumor invasion was characterized and quantified at the phenotypic level using hematoxylin and eosin and Ki-67 histology stains. Crystallin alpha B (CRYAB) and CD44 were identified as regulators of tumor invasion from scRNA-seq transcriptomic data and validated in vitro, in vivo, and in a mouse GBM resection model.ResultsAt the cellular level, we found that invasive GBM are less dense and proliferative than their non-invasive counterparts. At the molecular level, we identified unique transcriptomic features that significantly contribute to GBM invasion. Specifically, we found that CRYAB significantly contributes to postoperative recurrence and is highly co-expressed with CD44 in invasive GBM samples.ConclusionsCollectively, our analysis identifies differentially expressed features between invasive and nodular GBM, and describes a novel relationship between CRYAB and CD44 that contributes to tumor invasiveness, establishing a cellular and molecular landscape of GBM invasion.
Discovery of a new supergiant fast X-ray transient MAXI J0709−159 associated with the Be star LY Canis Majoris
Authors: Sugizaki Mutsumi , Mihara Tatehiro , Kobayashi Kohei , Negoro Hitoshi , Shidatsu Megumi , Pike Sean N , Iwakiri Wataru , Urabe Sota , Serino Motoko , Kawai Nobuyuki , Nakajima Motoki , Kennea Jamie A , Liu Zhu 
Published: July 2022

Abstract
We report on the discovery of a new supergiant fast X-ray transient (SFXT), MAXI J0709−159, and its identification with LY CMa (also known as HD 54786). On 2022 January 25, a new flaring X-ray object, named MAXI J0709−159, was detected by the Monitor of All-sky X-ray Image (MAXI). Two flaring activities were observed in two scans ∼3 hr apart, where the 2–10 keV flux reached 5 × 10−9 erg cm−2 s−1. During the period, the source exhibited a large spectral change, suggesting that the absorption column density NH increased from 1022 to 1023 cm−2. A NuSTAR follow-up observation on January 29 identified a new X-ray source with a flux of 6 × 10−13 erg cm−2 s−1 at a position consistent with LY CMa, which has been identified as a B supergiant as well as a Be star, located at a 3 kpc distance. The observed X-ray activity, characterized by short (≲several hours) duration, rapid (≲ a few seconds) variabilities accompanied by spectral changes, and a large luminosity swing (1032–1037 erg s−1), agree with those of SFXTs. On the other hand, optical spectroscopic observations of LY CMa reveal a broad Hα emission line, which may indicate the existence of a Be circumstellar disk. These results suggest that the optical companion, LY CMa, certainly has a complex circumstellar medium including dense clumps.
Psychometric Properties of the Patient-Reported Outcome Measures for People With Ulnar Nerve Entrapment at the Elbow: A Systematic Review
Authors: Dabbagh Armaghan , Saeidi Sahar , MacDermid Joy C 
Published: July 2022

Abstract

Objective
The objective of this study was to systematically review, critically appraise, and synthesize evidence on the measurement properties of patient-reported outcome measures (PROMs) for people with ulnar nerve entrapment at the elbow (UNE).

Methods
CINAHL, MEDLINE, Embase, and Scopus were searched for studies reporting on the psychometric properties of the PROMs used to assess pain, disability, and quality of life of people with UNE. Relevant data were extracted based on the Consensus-Based Standards for the Selection of Health Measurement Instruments 2018 checklist. Two authors independently rated the quality and risk of bias of the studies. A qualitative synthesis was performed according to the Consensus-Based Standards for the Selection of Health Measurement Instruments guidelines. The results were categorized based on the identified PROMs, and all the psychometric properties were summarized and presented.

Results
Eight included studies had a quality score of very good or higher. Among all the included PROMs, the Patient-Rated Ulnar Nerve Evaluation (PRUNE) was comprehensively evaluated in 4 studies and had high reliability, appropriate correlation to the physical component summary score of the 36-Item Short-Form Health Survey (r = −0.68), excellent content (cognitive interviews), structural (factor analysis) and discriminative validities, and high responsiveness. The Disabilities of the Arm, Shoulder and Hand Questionnaire was assessed in 5 studies and had appropriate properties, showing a high correlation with the 8-Item Short-Form Health Survey (r = 0.77) and PRUNE (r = 0.75) and moderate responsiveness.

Conclusions
The PRUNE was the most specific to UNE, had the most supporting studies and evidence of excellent reliability, validity, and responsiveness and therefore was the preferred PROM. The Disabilities of the Arm, Shoulder and Hand Questionnaire demonstrated more limited validation across 5 studies and had moderate responsiveness. Information on other included PROMs was limited in terms of psychometric properties.

Impact
This study provides insight into which PROM is potentially more suitable for measuring outcomes related to UNE.
COCOPLOT: COlor COllapsed PLOTting software Using colour to view 3D data as a 2D image
Authors: Druett Malcolm K , Pietrow Alexander G M , Vissers Gregal J M , Robustini Carolina , Calvo Flavio 
Published: July 2022

ABSTRACT
Most modern solar observatories deliver data products formatted as 3D spatiotemporal data cubes, that contain additional, higher dimensions with spectral and/or polarimetric information. This multidimensional complexity presents a major challenge when browsing for features of interest in several dimensions simultaneously. We developed the COlor COllapsed PLOTting (COCOPLOT) software as a quick-look and context image software, to convey spectral profile or time evolution from all the spatial pixels (x, y) in a 3D [nx, ny, nλ] or [nx, ny, nt] data cube as a single image, using colour. This can avoid the need to scan through many wavelengths, creating difference and composite images when searching for signals satisfying multiple criteria. Filters are generated for the red, green, and blue channels by selecting values of interest to highlight in each channel, and their weightings. These filters are combined with the data cube over the third dimension axis to produce an nx × ny × 3 cube displayed as one true colour image. Some use cases are presented for data from the Swedish 1-m Solar Telescope and Interface Region Imaging Spectrograph, including H α solar flare data, a comparison with k-means clustering for identifying asymmetries in the Ca ii K line and off-limb coronal rain in IRIS C ii slit-jaw images. These illustrate identification by colour alone using COCOPLOT of locations including line wing or central enhancement, broadening, wing absorption, and sites with intermittent flows or time-persistent features. COCOPLOT is publicly available in both idl and python.
Is Omega-3 Index necessary for fish oil supplements for CVD risk prevention?
Authors: Luo Chen , Chen Zhenyue 
Published: July 2022

Abstract
Several large prospective cohort studies demonstrated an association between higher cardiovascular disease (CVD) risk with low blood level of omega-3 fatty acids as well as low Omega-3 Index [<4% eicosapentaenoic acid (EPA)+ docosahexaenoic acid (DHA) to total fatty acids in red blood cell membrane]. However, randomized controlled trials of omega-3 fatty acids as either primary or secondary prevention have yielded controversial results. In this review, we summarize the evidence that supports or argues against the use omega-3 fatty acids, with a focus on the underlying mechanisms for the observed discrepancies (eg, differences in dosage, comparators and EPA levels or Omega-3 Index). Omega-3 Index is an independent risk factor for cardiovascular risk. The baseline Omega-3 Index can be used as a reference for whether and how much fish oil should be supplemented. To some degree, it can be used to explain why there are so much inconsistencies in clinical trials. Omega-3 Index could be a promising treatment target in clinical practice and in public health settings although there are still some barriers.
This review summarizes current evidences from both epidemiological studies and randomized controlled trials of omega-3 fatty acids as primary and secondary prevention of CVD, and aims to provide a comprehensive overview of fish oil supplements on risk for CVD, and Omega-3 Index as a tool to identify subjects at high risk as well as a treatment target in CVD prevention.
Bony Cartilaginous Graft in Unilateral Cleft Lip Rhinoplasty
Authors: Nguyen Chuong Dinh , Nguyen Tho Thi-Kieu , Tran Son Thiet , McDevitt Annette S. , Hodges John M. 
Published: July 2022

Background:Cleft rhinoplasty is a challenging form of nasal correction of both esthetic and functional deformities. The septal cartilage in many Asian patients are not sufficient and weak. Does a combination of the septal cartilage and the bony septum have both esthetic and functional benefits to secondary unilateral cleft rhinoplasty?Patients and Methods:Thirty patients with a unilateral cleft lip palate underwent open rhinoplasty from October 2018 to January 2021. After preserving a 10 mm L-strut, the posterior cartilaginous and bony septum were harvested as an integrative unit. The osteocartilaginous graft was used as a caudal septal extension graft and an extended spreader graft. Correcting the asymmetry of the tip and tip projection followed. The intraoperative harvested composite graft was analyzed. Acoustic rhinometry and the 3-dimensional anthropometric measurements of the external nose were assessed before and after surgery.Results:The osteocartilaginous unit was much larger than the cartilaginous part of this unit. The mean nasal tip height and the nasolabial angle increased significantly after surgery. The measurement of cross-sectional areas and volumes by acoustic rhinometry revealed that septorhinoplasty provided a significant increase in the function of both nasal cavities.Conclusions:This septal bony cartilaginous graft is effective for cleft lip nasal deformity when correcting the deviated septum, creating a supporting frame to correct the nasal tip asymmetry, improving function.
Multi-omics Analysis Identifying CD58 Plays Important Role on Prognosis of Colorectal Cancer
Authors: Wang Xuan 
Published: July 2022

AbstractThe immunotherapy is a programming therapy of cancer, so new immunotherapy target genes would be urgently demanded at present. Although The Cancer Genome Atlas Program (TCGA) provided more than 11,000 patients’ multi-omics data of more than 33 cancers, the heterogeneity of tumor tissue may still mask the immune-associated gene differential expression. In our study, to address the heterogeneity masked by the bulk RNA-Seq, TCGA data were first dividend into 26 types cancers by their high immune infiltration and low immune infiltration through single-sample gene set variation analysis to detect immune-associated gene, and single-cell RNA-Seq were than reanalyzed to prove the result we received in RNA-Seq. In order to verify our result in cell lines, a series of experiments were performed on two different microsatellite state cell lines: HCT116 (microsatellite instability high) and HT29 (microsatellite stability) because of colorectal cancer had its corresponding cell lines. Our results suggested that high expression levels of CD58, an immune-related gene, was masked by the bulk RNA-Seq and had a significant effect on the prognosis of patients with microsatellite instability high. The high expression of CD58 inhibited the proliferation and invasion of cancer cells in HCT116, which had highly unstable microsatellites, but had no effect on HT29, which had stable microsatellites. The high CD58 expression combined with microsatellite instability high statuses were the important molecular targets in the selection of immunotherapy methods in colorectal cancer. In short, our study provides new insight into detecting immune-associated gene in different cancer bulk RNA-Seq.
Understanding the state-dependent impact of species correlated responses on community sensitivity to perturbations
Authors: Medeiros Lucas P. , Saavedra Serguei 
Published: July 2022

AbstractUnderstanding how communities respond to perturbations requires us to consider not only changes in the abundance of individual species, but also correlated changes that can emerge through interspecific effects. However, our knowledge of this phenomenon is mostly constrained to populations at equilibrium, where interspecific effects are fixed. Here, we introduce a framework to disentangle the impact of species correlated responses on community sensitivity to perturbations when interspecific effects change over time due to non-equilibrium dynamics. We partition the volume expansion rate of perturbed abundances (community sensitivity) into contributions of individual species and of species correlations by converting the time-varying Jacobian matrix containing interspecific effects into a time-varying covariance matrix. Using population dynamics models, we demonstrate that species correlations change considerably across time and continuously alternate between reducing and having no impact on community sensitivity. Importantly, these alternating impacts depend on the abundance of particular species and can be detected even from noisy time series. We showcase our framework using two experimental predator-prey time series and find that the impact of species correlations is modulated by prey abundance—as theoretically expected. Our results provide new insights into how and when species interactions can dampen community sensitivity when abundances fluctuate over time.
Morphine Suppresses Peripheral Responses and Transforms Brain Myeloid Gene Expression to Favor Neuropathogenesis in SIV Infection
Authors: Fox Howard S. , Niu Meng , Morsey Brenda M. , Lamberty Benjamin G. , Emanuel Katy M. , Periyasamy Palsamy , Callen Shannon , Acharya Arpan , Kubik Gregory , Eudy James , Guda Chittibabu , Dyavar Shetty Ravi , Fletcher Courtney V. , Byrareddy Siddappa N. , Buch Shilpa 
Published: July 2022

ABSTRACTThe twin pandemics of opioid abuse and HIV infection can have devastating effects on physiological systems, including on the brain. Our previous work found that morphine increased the viral reservoir in the brains of treated SIV-infected macaques. In this study, we investigated the interaction of morphine and SIV to identify novel host-specific targets using a multimodal approach. We probed systemic parameters and performed single-cell examination of the targets for infection in the brain, microglia and macrophages. Morphine treatment created an immunosuppressive environment, blunting initial responses to infection, which persisted during antiretroviral treatment. Antiretroviral drug concentrations and penetration into the cerebrospinal fluid and brain were unchanged by morphine treatment. Interestingly, the transcriptional signature of both microglia and brain macrophages was transformed to one of a neurodegenerative phenotype. Notably, the expression of osteopontin, a pleiotropic cytokine, was significantly elevated in microglia. This was especially notable in the white matter, which is also dually affected by HIV and opioids. Increased osteopontin expression was linked to numerous HIV neuropathogenic mechanisms, including those that can maintain a viral reservoir. The opioid morphine is detrimental to SIV/HIV infection, especially in the brain.
A mucin-regulated adhesin determines the intestinal biogeography and inflammatory character of a bacterial symbiont
Authors: Smith T. Jarrod , Sundarraman Deepika , Melancon Ellie , Desban Laura , Parthasarathy Raghuveer , Guillemin Karen 
Published: July 2022

SUMMARYIn a healthy gut, microbes are often aggregated with host mucus, yet the molecular basis for this organization and its impact on intestinal health are unclear. Mucus is a viscous physical barrier separating resident microbes from epithelia, but also provides glycan cues that regulate microbial behaviors. Using experimental evolution, we discovered a mucin-sensing pathway in an Aeromonas symbiont of zebrafish, Aer01. In response to the mucin-associated glycan N-acetylglucosamine, a sensor kinase regulates expression of a mucin-binding adhesin we named MbpA. When MbpA is disrupted, Aer01 colonizes to normal levels, but is largely planktonic and elicits increased intestinal inflammation, traits which are reversed by increasing cell surface MpbA. MbpA-like adhesins are common in human-associated bacteria and expression of an Akkermansia muciniphila MbpA-like adhesin in MbpA-deficient Aer01 restored lumenal aggregation and reversed its pro-inflammatory character. Our work demonstrates how resident bacteria use mucin glycans to modulate behaviors congruent with host health.
Epigenetic Modifier Kdm6a/Utx Controls the Specification of Hypothalamic Neuronal Subtypes in a Sex-Dependent Manner
Authors: Cabrera Zapata Lucas E. , Cambiasso María Julia , Arevalo Maria Angeles 
Published: July 2022

ABSTRACTKdm6a is an X chromosome-linked H3K27me2/3 demethylase that promotes chromatin accessibility and gene transcription and is critical for tissue/cell-specific differentiation. Previous results showed higher Kdm6a levels in XX than in XY hypothalamic neurons and a female-specific requirement for Kdm6a in mediating increased axogenesis before brain masculinization. Here we explored the sex-specific role of Kdm6a in the specification of neuronal subtypes in the developing hypothalamus. Hypothalamic neuronal cultures were established from sex-segregated E14 mouse embryos and transfected with siRNAs to knockdown Kdm6a expression (Kdm6a-KD). We evaluated by immunofluorescence the effect of Kdm6a-KD on Ngn3, a bHLH transcription factor regulating neuronal sub-specification in hypothalamus. Kdm6a-KD decreased Ngn3 expression in females but not in males, abolishing basal sex differences. Then, we analyzed Kdm6a-KD effect on Ascl1, Pomc, Npy, Sf1, Gad1 and Th expression by RT-qPCR. While Kdm6a-KD downregulated Ascl1 in both sexes equally, we found sex-specific results for Pomc, Npy and Th. Pomc and Th expressed higher in female than in male neurons and Kdm6a-KD reduced their levels only in females, while Npy expressed higher in male than in female neurons and Kdm6a-KD upregulated its expression only in females. Identical results were found by immunofluorescence for Pomc and Npy neuropeptides. Finally, using ChIP-qPCR we found higher H3K27me3 levels at Ngn3, Pomc and Npy promoters in male neurons, in line with Kdm6a higher expression and demethylase activity in females. These results indicate that Kdm6a plays a key sex-specific role in controlling the differentiation of neuronal populations regulating food intake and energy homeostasis.