bluuebunny/crossref_metadata_2025_split huggingface dataset:
RITA1 drives the growth of bladder cancer cells by recruiting TRIM25 to facilitate the proteasomal degradation of RBPJ
Authors: Tang Huancheng , Li Xiangdong , Jiang Lijuan , Liu Zefu , Chen Lei , Chen Jiawei , Deng Minhua , Zhou Fangjian , Zheng Xianchong , Liu Zhuowei 
Published: June 2022

AbstractBladder cancer (BC) is one of the most prevalent malignancies worldwide, but it lacks effective targeted therapy due to its elusive molecular mechanism. Therefore, it is important to further investigate the molecular mechanisms that mediate BC progression. By performing a tumor tissue–based gene microarray and shRNA library screening, we found that recombination signal binding protein for immunoglobulin kappa J region (RBPJ) interacting and tubulin associated 1 (RITA1) is crucial for the growth of BC cells. Moreover, RITA1 is aberrantly highly expressed in BC tissues and is also correlated with poor prognosis in patients with BC. Mechanistically, we determined that RITA1 recruits tripartite motif containing 25 (TRIM25) to ubiquitinate RBPJ to accelerate its degradation via proteasome, which leads to the transcriptional inhibition of Notch1 downstream targets. Our results suggest that aberrant high expression of RITA1 drives the growth of BC cells via the RITA1/TRIM25/RBPJ axis and RITA1 may serve as a promising therapeutic target for BC.
Antitumoral RNA‐targeted oligonucleotide therapeutics: The third pillar after small molecule inhibitors and antibodies
Authors: Taniguchi Hiroaki , Suzuki Yasunori , Imai Kohzoh , Adachi Yasushi 
Published: June 2022

AbstractOligonucleotide therapeutics, drugs consisting of 10–50 nucleotide‐long single‐ or double‐stranded DNA or RNA molecules that can bind to specific DNA or RNA sequences or proteins, include antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), microRNAs (miRNAs), aptamers, and decoys. These oligonucleotide therapeutics could potentially become the third pillar of drug development. In particular, ASOs and siRNAs are advanced tools that are widely used to silence gene expression. They are used in clinical trials, as they have high specificity for target mRNAs and non‐coding RNAs and limited toxicity. However, their clinical application remains challenging. Although chemotherapy has benefits, it has severe adverse effects in many patients. Therefore, new modalities for targeted molecular therapy against tumors, including oligonucleotide therapeutics, are required, and they should be compatible with diagnosis using next‐generation sequencing. This review provides an overview of the therapeutic uses of ASOs, siRNAs, and miRNAs in clinical studies on malignant tumors. Understanding previous research and development will help in developing novel oligonucleotide therapeutics against malignant tumors.
Chronic remote ischemic conditioning for symptomatic internal carotid or middle cerebral artery occlusion: A prospective cohort study
Authors: Li Sijie , Zhao Wenbo , Liu Guiyou , Ren Changhong , Meng Ran , Wang Yuan , Song Haiqing , Ma Qingfeng , Ding Yuchuan , Ji Xunming 
Published: June 2022

AbstractAimsRemote ischemic conditioning (RIC) has been demonstrated to reduce recurrent stroke in patients with intracranial artery stenosis. This study aimed to evaluate the effects of RIC in patients with the symptomatic internal carotid artery (ICA) or middle cerebral artery (MCA) occlusion.MethodsThis study is based on a high‐volume single‐center prospective cohort study in China, which included patients with symptomatic ICA or MCA occlusion with impaired hemodynamics and receiving chronic RIC. Clinical follow‐up visits were performed regularly, and cardio‐cerebrovascular events were assessed.ResultsIn total, 131 patients (68 with ICA occlusion and 63 with MCA occlusion; mean age, 52.6 ± 13.7 years; stroke, 73.5%; transient ischemic attack TIA, 26.5%) qualified for the analysis; the mean follow‐up period was 8.8 years (range, 3–14 years). The compliance of RIC was 95.6 ± 3.7%, and no associated severe adverse events happened. The annual risk of ischemic stroke and ischemic cerebrovascular events was 2.4% and 3.3%, respectively. The cumulative probabilities of ischemic cerebrovascular events and major adverse cardiovascular and cerebrovascular events were 32.8% and 44.8% at 14 years, respectively.ConclusionIn patients with symptomatic ICA or MCA occlusion with impaired hemodynamics, chronic RIC is well‐tolerated, and it appears to be associated with a low annual risk of ischemic stroke and cardio‐cerebrovascular events.
Chlorophylls, lutein and β‐carotene play great but different roles in colour of rapeseed oil
Authors: Yao Yingzheng , Xuan Pu , Xiong Wei , Zhao Ling , Xu Xia , Liang Qiang 
Published: June 2022

AbstractNatural colourants have great effects on the colour of vegetable oil. A series of colourant–oil simulation systems were constructed to study the effects of chlorophyll, lutein and β‐carotene and their interactions on the colour of rapeseed oil. CIE L*a*b* and Lovibond RYBN colour codes were used to evaluate the colour of the simulation systems. There were good negative linear correlations between the concentrations of the three natural colourants and L* value in both single colourant–oil and two colourants–oil simulation systems. Chlorophyll had greater effect on lightness of rapeseed oil than lutein and β‐carotene. The contribution of the two carotenoids to the yellow colour was greater than that of chlorophyll. Lutein and β‐carotene contributed positively to the red colour of rapeseed oil. They had superposition effect on colour of rapeseed oil, and β‐carotene might contribute more to the colour than lutein. It provided a method to evaluate the contribution of colourant to the colour and a possibility to regulate oil colour in edible oil industry.
Concentration‐dependent response of soil parameters and functions to trifluoroacetic acid
Authors: Xu Baile , Alizray Rabea , Lammel Daniel R. , Riedel Sebastian , Rillig Matthias C. 
Published: June 2022

AbstractDespite growing environmental concerns about perfluoroalkyl and polyfluoroalkyl substances (PFAS) worldwide, the ultra‐short‐chain PFAS, for example, trifluoroacetic acid (TFA), have been largely neglected in the context of soil pollution. Given the persistence and increasing occurrence of TFA in the soil environment, accumulated TFA could impact soil properties and functions. Therefore, we investigated the effects of a wide range of concentrations of TFA (0.001–100 μg g−1) on chemical, physical and biological indicators of soil health, using a six‐week microcosm experiment. Our results showed that TFA treatments decreased soil pH, sulphate content, soil respiration, litter decomposition and bacterial abundance, and increased phosphate content (p < 0.05). These effects were observed for TFA concentrations ≥1 μg g−1. As expected, because of its strong acidity, TFA decreased soil pH, and this change likely contributed to effects on other soil parameters and functions, for example, reducing bacterial abundance and soluble nutrients. However, importantly, TFA clearly also affected soil parameters at concentrations at which soil pH was not changed. Soil aggregation, fungal abundance, and enzyme activities were not affected by TFA in this study. Considering the current reported environmental levels of TFA of <2.4 μg g−1, only litter decomposition would be affected under current field conditions. Our data also show that future accumulation of higher concentrations, or hotspots of TFA, will likely negatively affect soils with similar properties as our test soil. Thus, we demonstrated that microbial processes can be impacted by the accumulation of the ‘forever chemical’ TFA in the tested sandy, low organic matter soil.HighlightsTrifluoroacetic acid (TFA) decreased soil pH, respiration, litter decomposition and bacterial abundance.TFA affected soil soluble nutrients.TFA affected litter decomposition at 1 μg g−1with no change in soil pH.Effects of TFA on soil enzyme activity and aggregation and fungal abundance were negligible.
Anterior nucleus of the thalamus deep brain stimulation vs temporal lobe responsive neurostimulation for temporal lobe epilepsy
Authors: Yang Jimmy C. , Bullinger Katie L. , Dickey Adam S. , Karakis Ioannis , Alwaki Abdulrahman , Cabaniss Brian T. , Winkel Daniel , Rodriguez‐Ruiz Andres , Willie Jon T. , Gross Robert E. 
Published: June 2022

AbstractObjectiveBased on the promising results of randomized controlled trials, deep brain stimulation (DBS) and responsive neurostimulation (RNS) are used increasingly in the treatment of patients with drug‐resistant epilepsy. Drug‐resistant temporal lobe epilepsy (TLE) is an indication for either DBS of the anterior nucleus of the thalamus (ANT) or temporal lobe (TL) RNS, but there are no studies that directly compare the seizure benefits and adverse effects associated with these therapies in this patient population. We, therefore, examined all patients who underwent ANT‐DBS or TL‐RNS for drug‐resistant TLE at our center.MethodsWe performed a retrospective review of patients who were treated with either ANT‐DBS or TL‐RNS for drug‐resistant TLE with at least 12 months of follow‐up. Along with the clinical characteristics of each patient's epilepsy, seizure frequency was recorded throughout each patient's postoperative clinical course.ResultsTwenty‐six patients underwent ANT‐DBS implantation and 32 patients underwent TL‐RNS for drug‐resistant TLE. The epilepsy characteristics of both groups were similar. Patients who underwent ANT‐DBS demonstrated a median seizure reduction of 58% at 12–15 months, compared to a median seizure reduction of 70% at 12–15 months in patients treated with TL‐RNS (p > .05). The responder rate (percentage of patients with a 50% decrease or more in seizure frequency) was 54% for ANT‐DBS and 56% for TL‐RNS (p > .05). The incidence of complications and stimulation‐related side effects did not significantly differ between therapies.SignificanceWe demonstrate in our single‐center experience that patients with drug‐resistant TLE benefit similarly from either ANT‐DBS or TL‐RNS. Selection of either ANT‐DBS or TL‐RNS may, therefore, depend more heavily on patient and provider preference, as each has unique capabilities and configurations. Future studies will consider subgroup analyses to determine if specific patients have greater seizure frequency reduction from one form of neuromodulation strategy over another.
Genetic inhibition of collapsin response mediator protein‐2 phosphorylation ameliorates retinal ganglion cell death in normal‐tension glaucoma models
Authors: Brahma Musukha Mala , Takahashi Kazuya , Namekata Kazuhiko , Harada Takayuki , Goshima Yoshio , Ohshima Toshio 
Published: June 2022

AbstractGlaucoma is a neurodegenerative disorder caused by the death of retinal ganglion cells (RGCs). Elevated intraocular pressure (IOP) is a cause of glaucoma. However, glaucoma often develops with normal IOP and is known as normal‐tension glaucoma (NTG). Glutamate neurotoxicity is considered as one of the significant causes of NTG, resulting in excessive stimulation of retinal neurons via the N‐methyl‐D‐aspartate (NMDA) receptors. The present study examined the phosphorylation of collapsin response mediator protein‐2 (CRMP2), a protein that is abundantly expressed in neurons and involved in their development. In two mouse models, NMDA‐injection and glutamate/aspartate transporter (GLAST) mutant, CRMP2 phosphorylation at the cyclin‐dependent kinase‐5 (Cdk5) site was elevated in RGCs. We confirmed that the decrease in the number of RGCs and thickness of the inner retinal layer (IRL) could be suppressed after NMDA administration in CRMP2KI/KI mice with genetically inhibited CRMP2 phosphorylation. Next, we investigated GLAST heterozygotes (GLAST+/−) with CRMP2KI/KI (GLAST+/−;CRMP2KI/KI) and GLAST knockout (GLAST−/−) mice with CRMP2KI/KI (GLAST−/−;CRMP2KI/KI) mice and compared them with GLAST+/− and GLAST−/− mice. pCRMP2 (S522) inhibition significantly reduced RGC loss and IRL thinning. These results suggest that the inhibition of CRMP2 phosphorylation could be a novel strategy for treating NTG.
Comparison of non‐volatile degradation products formed from different vegetable oils during deep frying of French fries
Authors: Chen Jia , Zhang Lingyan , Zhao Peng , Wang Jiayun , Li Qi , Yu Xiuzhu 
Published: June 2022

SummaryDeterioration of frying oil greatly affects its nutritional quality. This study sought to establish how oil type affected the evolution of non‐volatile degradation products in frying oils. Frying experiments were applied with three different commercial oils (palm oil, rapeseed oil, soybean oil) known to be used in French fries, and the formed degradation products in oils were determined by classical assays, Fourier transform infrared spectroscopy (FTIR) and 1H nuclear magnetic resonance (NMR). The FTIR demonstrated that the thermal stress may result in gradual alteration of cis fatty acids to trans fatty acids. The acyl groups, oxygenated compounds and 1,2‐diglycerides in samples were determined by 1H NMR. Soybean and rapeseed oils contained more oxygenated compounds than palm oil owing to higher polyunsaturated acyl group content. However, a contrary result was shown for 1,2‐diglycerides. Of note, the major aldehydes formed were n‐alkanals, (E,E)‐2,4‐alkadienals and (E)‐2‐alkenals; 4‐oxo‐alkanals were not detected in palm oil. Correlation analysis indicated that these aldehydes were positively correlated with the polymerisation and oxidation of frying oils (P < 0.01). The research provided new data for evaluating the suitability of these oils for food preparations.
Strain effects in epitaxial La‐doped SrSnO3 films on lattice‐matched PMN‐PT substrates
Authors: Li Kaifeng , Zhao Li , Gao Qiang , Lv Kai , Wang Haifeng , Liu Qinzhuang 
Published: June 2022

AbstractHere we report the effect of the strain states on the structure, optical and electrical transport properties of the La0.05Sr0.95SnO3 (LSSO) thin films grown epitaxially on (001)‐oriented 0.70 Pb(Mg1/3Nb2/3)O3‐0.30PbTiO3 (PMN‐PT) substrates by pulsed laser deposition. X‐ray diffraction results indicate that the films are fully strained up to at least 100 nm thickness, and the in‐plane compressive strain gradually releases in thicker films. High‐resolution transmission electron microscopy characterizations demonstrate that the LSSO films were grown coherently on PMN‐PT(001) substrates. With varying the thicknesses of the fully strained films from 20 to 100 nm, the electrical transport properties are improved significantly. A lowest room‐temperature resistivity of 1.88 mΩcm and the highest mobility of 28.1 cm2/Vs are obtained in the 100 nm film. The optical band gap determined from spectroscopic ellipsometry is found to increase from 4.58 to 4.88 eV with the film thicknesses varying from 20 to 500 nm. The results imply that the LSSO epitaxial films exhibit tunable electrical performances and optical band gaps through strain, which may have potential applications in optoelectrical devices.
Comparison of the efficacy of periodontal prognostic systems in predicting tooth loss
Authors: Saydzai Selai , Buontempo Zoe , Patel Pankti , Hasan Fatemah , Sun Chuanming , Akcalı Aliye , Lin Guo‐Hao , Donos Nikos , Nibali Luigi 
Published: June 2022

AbstractAimThe aim of this analysis was to assess how different tooth‐prognosis systems could predict tooth loss in a cohort of periodontitis patients followed up prospectively during supportive periodontal care (SPC).Materials and MethodsClinical and radiographic data of 97 patients undergoing regular SPC for 5 years were used to assign tooth prognosis using four different systems (McGuire & Nunn, 1996; Kwok & Caton, 2007; Graetz et al., 2011; Nibali et al., 2017). Three independent examiners assigned tooth prognosis using all four systems, following a calibration exercise. The association between prognostic categories and tooth loss was tested for each prognostic system separately and across prognostic systems.ResultsAll four systems showed good reproducibility and could identify teeth at higher risk of being lost during 5 years of SPC; the risk of tooth loss increased with the worsening of tooth‐prognosis category (p < .0001). Although specificity and negative predictive values were good, low sensitivity and positive predictive values were detected for all systems.ConclusionsPreviously published periodontal prognostic systems exhibited good reproducibility and predictive ability for tooth retention. However, low sensitivity was detected, with several teeth in the worst prognosis category being retained at 5 years. Some modifications in the number of categories and their definitions are suggested.
Numerical modeling and experimental validation of meat burgers and vegetarian patties cooking process with an innovative IR laser system
Authors: Schillaci Eugenio , Gràcia Alvar , Capellas Marta , Rigola Joaquim 
Published: June 2022

AbstractIn this paper, a numerical model for the simulation of the cooking process of meat burgers and vegetarian patties by means of an IR laser beam is presented. The innovative cooking system is thought to be integrated within a 3‐D printer prototype where the food sample is placed on a rotating plate. The numerical model consists of two different tools. First, a simplified 0‐D model, aimed at studying the heat transfer processes between the food sample, the lower plate, and the surrounding environment. Second, a 3‐D finite‐volume algorithm, which is employed to simulate the temporal and spatial evolution of the temperature within the food sample, when subjected to a laser beam which follows a given path. In parallel, an experimental test campaign is carried out to measure the temperature and cooking losses evolution inside burgers and patties. The results of the experimental campaign on the evolution of the temperature inside meat burgers and vegetarian patties are used to validate the numerical method, showing a good agreement. Next, the numerical method is used to carry out a parametric analysis of the IR laser system, aimed at analyzing and optimizing cooking parameters as laser power, incidence time and reversal time.Practical ApplicationThe current practical application of the numerical model presented here consists on the development of 3D food printers' prototypes which incorporate an infrared laser cooking system. IR laser cooking is a new cooking method currently under research which requires an adequate experimental design and an intense experimental work. The numerical models presented here allows an effective estimation of spatial and temporal distribution of real cooking temperatures during an infrared (IR) laser cooking process, allowing the analysis of pasteurization temperatures needed to inactivate pathogenic bacteria. The numerical simulation tool can be used to optimize and develop the IR laser system by analyzing different parametric conditions, as the ray tracing function frequency (incidence time), the reversal time of the burger, and the laser power. Simulations are aimed at simplifying and guiding the experimental work, leading to time and material savings, and it would also help to determine the most significant variables involved in the cooking process. The present model can be integrated as a new application of the laser control software and can be used in a professional gastronomic environment to choose between different cooking degrees, to analyze the characteristics of the printed food, or to determine the appropriate cooking parameters required to ensure food safety. The ability to 3D print and cook in the same physical area may facilitate a better implementation of the 3DFP technology in different markets.
Integrated clinical pathways for lower limb orthopaedic surgeries: An updated systematic review
Authors: Zhou Huaqiong , Ngune Irene , Roberts Pam A. , Della Phillip R. 
Published: June 2022

AbstractObjectiveThe objective of the study was to comprehensively synthesise the components of integrated clinical pathways (ICPs) and post‐operative outcomes of patients undergone total hip and knee arthroplasty (THA & TKA) and hip fracture surgeries.BackgroundPrevious systematic reviews examined components and effectiveness of ICPs for lower limb joint replacement and hip fracture surgeries.Design and MethodsAn updated systematic review guided by the Whittemore and Knafl (2005) framework. Electronic databases, Ovid MEDLINE, EBSCOhost‐CINAHL, the Cochrane Reviews and Trails, EMBASE and PubMed, were searched from 2007 to 31 January 2021. Due to the heterogeneity of the methods and data collection tools of included studies, pooling of the quantitative data was not possible. Therefore, the included studies were synthesised and presented narratively under subthemes of arthroplasty and hip fracture surgeries. The PRISMA checklist for systematic reviews was used.ResultsTwenty‐four studies met selection criteria with 11 examined ICPs for hip fracture and 13 for the THA and TKA. Twenty‐one ICPs were reviewed, and 33 components were extracted. The most frequently included components for hip fracture subgroup were ‘discharge disposition arrangement’ and ‘dedicated personnel and resources’. ‘Exercise plan’ and ‘pain management’ were for the arthroplasty subgroup. A significant reduction in the length of stay and post‐operative complications were associated with the ICPs. Results were mixed for the effectiveness of ICPs in reducing unplanned hospital admissions, mortality rates, post‐operative complications and hospital costs.ConclusionThe number of ICP components varied across studies. This review could not recommend a one size‐fits‐all ICP that could be adapted for use for patients undergoing hip fracture and joint replacement surgeries.Relevance for clinical practiceThis review identified research evidence‐based components considered as essential for the inclusion in ICP’s for hip fracture and arthroplasty surgeries. Further research is suggested to determine the patient experience and healthcare providers’ acceptance of ICPs.
Localized labor flow networks in knowledge‐intensive industries
Authors: Lahdelma Tamás 
Published: June 2022

AbstractThis study examines the relationship between agglomeration and labor market matching by investigating the small‐scale sociospatial determinants of a network created by the within‐region mobility of employees across organizations. The local labor flow network is constructed on the basis of an establishment‐level matched employer–employee database covering selected knowledge‐intensive industries in the Helsinki–Uusimaa Region in Finland during the period 2001–2015. The results from the analysis of various tie formation processes reveal that the formation of a link by interorganizational employee mobility within an integrated labor market area is more likely between organizations geographically closer to each other, all else being equal. The findings highlight the importance of local density for the job search process.