bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mnx1 Induces Leukemia Transformation Through Altering Histone Methylation in a Model of Pediatric Acute Myeloid Leukemia with t(7;12)(q36;p13)
Authors: Waraky Ahmed , Östlund Anders , Nilsson Tina , Weichenan Dieter , Lutsik Pavlo , Bähr Marion , Hey Joschka , Adamsson Jenni , Morsy Mohammad , Li Susann , Fogelstrand Linda , Plass Christoph , Palmqvist Lars 
Published: September 2022

Abstract
Certain subtypes of acute myeloid leukemia (AML) in children have still inferior outcome. One of these AML subtypes has a translocation t(7;12)(q36;p13), always leading to high expression of MNX1 and often to MNX1::ETV6 fusion expression. Here we identified the transforming event in this AML and possible ways to target them. Only MNX1 was able to induce AML in mice, and this was observed using hematopoietic stem and progenitor cells derived from fetal origin but not from adult bone marrow. The restriction in the transforming capacity to cells from fetal liver origin is in concordance with the fact that t(7;12)(q36;p13) AML is mostly restricted to infants. Ectopic expression of MNX1 led to increase of H3K4me1, H3K4me2 and H3K4me3, reduction in H3K27me3, accompanied with changes in genome-wide chromatin accessibility and genome expression, likely mediated through MNX1 interaction with the methionine cycle and different methyltransferases. MNX1 expression resulted in increased DNA damage, depletion of the Lin-/Sca1+/c-Kit+ population and skewing toward the myeloid lineage. These effects, together with leukemia development, was prevented by the S-adenosylmethionine analog Sinefungin. In conclusion, we have shown the importance of MNX1 in leukemia development in AML with t(7;12), supporting a rationale for targeting MNX1 and downstream pathways.
CT-Based Deep Learning Enables Chemotherapy Response Prediction for Pancreatic Ductal Adenocarcinoma: A Pilot Study
Authors: Kanda Taishu , Wakiya Taiichi , Ishido Keinosuke , Kimura Norihisa , Nagase Hayato , Yoshida Eri , Nakagawa Junichi , Matsuzaka Masashi , Niioka Takenori , Sasaki Yoshihiro , Hakamada Kenichi 
Published: September 2022

Abstract
Background
Predicting response to chemotherapy can lead to its optimization. Furthermore, prediction using a noninvasive approach is clearly preferable to an invasive approach. This study aimed to predict in vitro chemosensitivity assay results by combining computed tomography (CT) images and deep learning (DL) to optimize chemotherapy for pancreatic ductal adenocarcinoma (PDAC)
Methods
We collected the dataset of preoperative CT images and the histoculture drug response assay (HDRA) of 33 patients undergoing surgery for PDAC at our facility. We trimmed small patches from the entire tumor area, using the data augmentation technique, and obtained 10,730 patches. We established various prediction labels for 5-fluorouracil (FU), gemcitabine (GEM), and paclitaxel (PTX). We built a predictive model using a residual convolutional neural network and used 3-fold cross-validation.
Results
Of the 33 patients, effective response to FU, GEM, and PTX by HDRA was observed in 19 (57.6%), 11 (33.3%), and 23 (88.5%) patients, respectively. The average accuracy and the area under the receiver operating characteristic curve (AUC) of the model for predicting the effective response to FU were 93.4% and 0.979, respectively. In the prediction of GEM, the models demonstrated high accuracy (92.8%) and AUC (0.969). Likewise, the model for predicting response to PTX had a high performance (accuracy 95.9%, AUC 0.979).
Conclusions
Our CT-patch-based DL model exhibited high predictive performance in projecting HDRA results. Our study suggests that the DL approach could possibly provide a noninvasive means for the optimization of chemotherapy.
The cause of discoid meniscus: a genomic exploring research
Authors: Lin Jianping , Zhang Jian , Zhou Gang , Wu Yongchang , Wang Benchao 
Published: September 2022

Abstract
Background: Discoid meniscus is a congenital structural variation of the meniscus. But, its pathogenesis is still unknown. This study aims to find the related genes of this disease through genomics and bioinformatics methods.
Methods and Material: In this study, we recruited 10 patients who were diagnosed with discoid meniscus. DNA were extracted peripheral venous blood from patients respectively, and were broken to fragments about 250-300bp. DNA fragments accepted repairing, connecting, purifying and PCR. The whole exon sequencing performed at these fragments via sequencing platform. The data which output by the platform were dealt with some bioinformatic tools. Last, the genetic and protein damage prediction were put down to variant.
Statistical analysis used: Mann-Whitney U test, and the statistical significance is P<0.05, and 95% confidence was calculated.
Results: The discrepancy of the counts between male and female group is inconspicuous(P<0.05). Based on bioinformatic analysis, we found 9 pathological gene (AFF1, KCTD1, NRF1, PADI4, FLNB, SYNE1, MYO9A, COL11A2 and COL2A1). Although
10 common variants were founded in this study, 7 variants exert subtle on protein. The most harmful sites belong to AFF1, KCTD1, NRF1, PADI4, FLNB, SYNE1, MYO9A.
Conclusions: We screened a total of 9 suspected pathogenic genes. we supposed that the formation of the discoid meniscus is not caused by a single gene mutation.
Thermoelectric properties of topological chains coupled to a quantum dot
Authors: Figueira M S , Continentino Mucio A , Lima A C P , Ribeiro R C Bento , Deus Fernanda , Correa J H 
Published: September 2022

Abstract
Topological one-dimensional superconductors can sustain zero energy modes protected by different kinds of symmetries in their extremities. Observing these excitations in the form of Majorana fermions is one of the most intensive quests in condensed matter physics. We are interested in another class of one-dimensional topological systems in this work, namely topological insulators. Which present symmetry-protected end modes with robust properties and do not require the low temperatures necessary for topological superconductivity. We consider a device in the form of a single electron transistor coupled to the simplest kind of topological insulators, namely chains of atoms with hybridized sp orbitals. We study the thermoelectric properties of the device in the trivial, non-trivial topological phases and at the quantum topological transition of the chains. We show that the device's electrical conductance and the Wiedemann-Franz ratio at the topological transition have universal values at very low temperatures. The conductance and thermopower of the device with diatomic sp-chains, at their topological transition, give direct evidence of fractional charges in the system. The former has an anomalous low-temperature behavior, attaining a universal value that is a consequence of the double degeneracy of the system due to the presence of zero energy modes. On the other hand, the system can be tuned to exhibit high values of the thermoelectric figure of merit and the power factor at high temperatures.
Sub-lethal doses of chemotherapeutic agents induce senescence in T cells and upregulation of PD-1 expression
Authors: Kasamatsu Tetsuhiro , Awata-Shiraiwa Maaya , Ishihara Rei , Murakami Yuki , Masuda Yuta , Gotoh Nanami , Oda Tsukasa , Yokohama Akihiko , Matsumura Ikuko , Handa Hiroshi , Tsukamoto Norifumi , Murakami Hirokazu , Saitoh Takayuki 
Published: September 2022

Abstract
Cellular senescence refers to a pause in the cell cycle, usually in response to internal and/or external stress, including telomere dysfunction, abnormal cellular growth, and DNA damage. Several chemotherapeutic drugs, such as melphalan (MEL) and doxorubicin (DXR), induce cellular senescence in cancer cells. However, it is not clear whether these drugs induce senescence in immune cells. We evaluated the induction of cellular senescence in T cells were derived from human peripheral blood mononuclear cells (PBMNCs) in healthy donors using sub-lethal doses of chemotherapeutic agents. The PBMNCs were kept overnight in RPMI 1640 medium with 2% phytohemagglutinin and 10% fetal bovine serum and then cultured in RPMI 1640 with 20 ng/mL IL-2 and sub-lethal doses of chemotherapeutic drugs (2 µM MEL and 50 nM DXR) for 48 h. Sub-lethal doses of chemotherapeutic agents induced phenotypes associated with senescence, such as the formation of γH2AX nuclear foci, cell proliferation arrest, and induction of senescence-associated beta-galactosidase (SA-β-Gal) activity, (control vs. MEL, DXR; median mean fluorescence intensity (MFI) 1883 (1130–2163) vs. 2233 (1385–2254), 2406.5 (1377–3119), respectively) in T cells. IL6 and SPP1 mRNA, which are senescence-associated secretory phenotype (SASP) factors, were significantly upregulated by sublethal doses of MEL and DXR compared to the control (P = 0.043 and 0.018, respectively). Moreover, sub-lethal doses of chemotherapeutic agents significantly enhanced the expression of programmed death 1 (PD-1) on CD3 + CD4 + and CD3 + CD8 + T cells compared to the control (CD4 + T cells; P = 0.043, 0.043, and 0.043, respectively, CD8 + T cells; P = 0.043, 0.043, and 0.043, respectively). Our results suggest that sub-lethal doses of chemotherapeutic agents induce senescence in T cells and tumor immunosuppression by upregulating PD-1 expression on T cells.
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
Authors: Khoshnami Maryam , Zare Bahar , Mardani-Mehrabad Hamideh , Rakhshandehroo Farshad , Baghery Mohammad Amin , Malboobi Mohammad Ali 
Published: September 2022

Abstract
Sugar beet is an economically important crop and one of the major sources of sucrose. Beet necrotic yellow vein virus (BNYVV) and Beet severe curly top virus (BSCTV) are two widespread viruses in sugar beet that cause severe damage to its performance. Previously, we have successfully achieved resistance to BNVYY by introducing coat protein-based DNA sequence constructs inducing gene silencing into sugar beet. However, the RNA silencing-mediated resistance of plants to a specific virus can be potentially broken down by another one as a part of synergistic interactions. In this study, we assayed the efficiency of the induced resistance of transgenic events to BNYVV and BSCTV-Ir under single or mixed infections. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was observed in S3 events based on mild symptoms and low PCR amplification of the BSCTV-Ir coat protein sequence. Similarly, partial resistance to BSCTV-Ir was detected in the same transgenic plants under co-infection conditions. Based on the presented data, resistance to BNYVV was stable in almost all the transgenic plants co-infected with BSCTV-Ir, except for one event (S3-229) that was broken down. Overall, it seems that the co-infection with BNYVV and BSCTV-Ir does not affect the resistance of transgenic plants to both viruses. These findings demonstrated that RNA silencing-based resistance to BNYVV introduced in transgenic sugar beets of this study is very efficient and is not easily weakened after co-infection with a heterologous virus.
Suitability evaluation of land reclamation for cultivated land in coal- mining area based on catastrophe progression method
Authors: LI Ying-hui 
Published: September 2022

Abstract

The suitability of land reclamation as cultivated land in coal mining area was studied, and a synthetic evaluation model was put forward based on the catastrophe progression method (CPM). The classification of land reclamation suitability status was divided into four grades: suitable, moderately suitable, less suitable and unsuitable. On the basis of comprehensive consideration of three factors including land quality, soil nutrients and engineering suitability in the reclamation area, eleven parameters including terrain slope grade, effective thickness of soil layer, soil parent materials, soil contamination, organic content, alkali hydrolyzable nitrogen (N), available phosphorus (P), available potassium (K), ground collapse, land destruction extent, and conditions of irrigation and drainage were selected as evaluation indicators, and the classification standards of each evaluation indicator were determined. Using MATLAB software to generate 800 total samples (200 samples per level) between the arrays corresponding to each level of standards according to the principle of normal distribution, of which 600 were used as training samples to establish the catastrophe progression criterion, and 200 were used as test samples to verify the reliability of the criterion, and the criterion was verified by engineering examples. The suitability of land reclamation for cultivated land in a coal mining area in Shaodong county, China, was comprehensively evaluated by using the proposed model based on CPM. The research results have reference and guiding significance for the comprehensive evaluation of the suitability of land reclamation for cultivated land in coal mining areas.
Fatty infiltration of multifidus muscle independently increases osteoporotic vertebral compression fracture risk
Authors: Lee Dong Gyu , Bae Jae Hwa 
Published: September 2022

AbstractBackground Vertebral compression fractures decrease daily life activities and increase economic and social burdens. In addition, sarcopenia and back muscle atrophy influence osteoporotic vertebral compression fractures (OVCF). Therefore, this study aimed to evaluate the influence of the multifidus muscle on the OVCF. Methods We retrospectively recruited the study population based on the hospital database following the inclusion and exclusion criteria. The inclusion criteria were: 1) ≥ 60 years and 2) concurrent bone mineral density (BMD) and lumbar spine magnetic resonance imaging (MRI). The exclusion criteria were: 1) a history of lumbar spinal surgery, 2) lumbar spine metastasis, and 3) systemic diseases affecting bone density, including chronic renal failure and liver cirrhosis. The participants were divided into three groups based on lumbar spine BMD and OVCF. The control group underwent BMD and spinal MRI evaluation but not OVCF. Based on the T-score of lumbar spine BMD, the fracture groups with OVCF were divided into osteopenia and osteoporosis groups. Osteopenia BMD groups showed osteopenia T-scores of spinal BMD of over − 2.5. Osteoporosis BMD groups also showed osteoporosis T-scores of lumbar BMD of -2.5 and below. Results We included 120 patients who had visited our hospital. Based on spinal MRI, 75 participants were diagnosed with OVCF, and 45 were not. Age, BMD, and the psoas index significantly differed between the control and fracture groups. Moreover, fatty infiltration of the multifidus muscle indifferently affected the OVCF, with and without adjusting for other significant factors. Conclusions The severity of fatty infiltration of the multifidus muscle increases the risk of a spinal fracture. Therefore, preserving the quality of the spinal muscle and bone density is essential for preventing OVCF.
Successful management of an unusual brain abscess with multiple locations: case report and review of literature
Authors: An Yuan , Song Dengpan , Guo Fuyou 
Published: September 2022

Abstract
Introduction: Intracranial abscesses at multiple locations are extremely rare and are associated with poor prognosis. High intracranial hypertension crisis and ventriculitis resulting from abscess rupture are fatal.
Case report:Here, we reported a 15-year male patient with uncommon intracranial abscess involving the bilateral frontal lobe, falx cerebral, left tentorium, left occipital, and temporal subdural locations. Successful treatment was achieved after three aspirations and subsequent systemic anti-infective drug administrations. This patient recovered uneventfully, and follow-up magnetic resonance imaging revealed that the lesions had almost completely disappeared.
Conclusion: This is an extremely rare case of brain abscess in multiple locations. Establishing a timely diagnosis, such as by several cultures of pus and genetic testing, is essential for the proper management of atypical cerebral abscesses.
Numerical analysis of mixed mode (I/II) stress intensity factors of single edge cracked/notched plates under different boundary conditions using strain energy approach
Authors: Shi Liang , Oyadiji S Olutunde 
Published: September 2022

Abstract

The stress intensity factor is a conventional parameter in Fracture Mechanics and there are some analytical solutions for many different fracture cases. This paper presents the mixed mode I/II stress intensity factors (SIF) of slanted cracks/notches under tension loading. A two-dimensional finite element analysis was employed using ABAQUS finite element program. Various crack lengths and angles were analysed. The effect of the different boundary conditions applied at the plate edge was also examined. Based on the literature survey, there is a lack of solution of SIFs of slanted cracks in plane stress plates. The strain energy approach (SEA) was adopted to analyse the notched cases since finite element software are not capable of computing the notched SIFs directly. By obtaining the strain energy in the FEA results, mode I and II SIFs can be calculated simply by using the SEA method. Moreover, the accuracy of this approach has been validated and compared with the available published results and shows an excellent agreement. The key contribution of the present paper is to evaluate the effect of the crack/notch length to plate width ratio on the mode I and II SIFs of a slant-edge-cracked/notched plate under different boundary conditions, i.e. pinned boundary condition and clamped boundary condition. Crack/notch length to plate width ratios from 0.1 to 0.9, slant angle from 0º to 45º, notch initial angle from 0º to 45º were considered. Series of new data of mode I and II SIFs are provided herein.
Characterization of circulating immune cells and correlation with Tie2/Angiopoietins level in well differentiated neuroendocrine gastroenteropancreatic tumors: a cross-sectional analysis
Authors: Sesti Franz , Puliani Giulia , Feola Tiziana , Campolo Federicas , Sciarra Francesca , Hasenmajer Valeria , Lenzi Andrea , Faggiano Antongiulio , Isidori Andrea Marcello , Venneri Mary Anna , Giannetta Elisa 
Published: September 2022

AbstractPurpose:The immune environment represents a new, but little explored, tool for understanding neuroendocrine neoplasms (NENs) behavior. An immunosuppressed microenvironment is hypothesized to promote NENs progression. A missing profiling of circulating leukocyte and peripheral blood mononuclear cell (PBMC) subpopulations would open new perspectives in the still limited diagnostic-therapeutic management of NENs.Methods: A cross-sectional case-control pilot study was performed recruiting 30 consecutive patients: 15 naïve to treatment, with histologically proven gastroenteropancreatic (GEP) neuroendocrine tumors (NETs) and 15 healthy controls (Ctrl), matched for age and sex. PBMC subpopulations were studied by flow cytometry. Soluble Tie2 (sTie2), Angiopoietin-1 (Ang-1), Angiopoietin-2 (Ang-2) were evaluated by ELISA.Results: Immune cell profiling revealed a significant lower CD3-CD56+ natural killer (NK) cell count in NETsversusCtrl (p=0.04). NK subset analysis showed a reduced relative count of CD56+CD16+ NK cells (p=0.002) in NETsversusCtrl. Patients with NET showed a higher percentage of CD14+CD16++non-classical monocytes (p=0.01), and a lower percentage of CD14+CD16+intermediate monocytes (p=0.04). A decrease in percentage (p=0.004) of CD4+ T-helper lymphocytes was found in NETs patients. Evaluation of cellular and serum angiopoietin pathway mediators revealed in NET patients a higher relative count of TEM (p<0.001), and high levels of Ang-1 (p=0.003) and Ang-2 (p=0.002).Conclusions: patients with GEP-NETs presented an immunosuppressed environment characterized by a low count of cytotoxic NK cells, a high count of anti-inflammatory non-classical monocytes, and a low count of T-helper lymphocytes. Higher levels of TEM and angiopoietins suggest a crosstalk between innate immunity and angiogenic pathways in NETs.
Association and interaction between metal aluminum and iron exposure and the risk of offspring’ congenital heart defects in northwestern China
Authors: Li Jing , Zhang Chunhua , Mao Baohong , Liu Qian , Wang Yanxia , Dai Zhirong , Yi Bin , Liu Qing 
Published: September 2022

Abstract
Congenital heart disease (CHDs) is the leading cause of mortality from birth defects, affecting up to 1% of live births. However, there have been inconsistent results of the relationship between aluminum (Al) /iron (Fe) level and risk of CHDs. We performed a pair-matched case–control study and included 97 cases and 194 controls to investigate the association and interaction between Al/Fe exposure and the risk of CHDs in a birth cohort study conducted in Lanzhou, China. Compared to < 25th percentile of Al in CB, risk differences were significantly detected in > 75th percentile of Al in total CHDs (aOR = 2.826, 95% CI: 1.009–7.266) and isolated CHDs (aOR = 10.713, 95% CI: 1.017–112.851). Although a similar pattern was not detected in MB of Al and Fe in both MB and CB, a significant dose-effect was observed between the Al level and total CHDs in both MB and CB (Ptrend < 0.05). Compared to both lowest tertile of Al and Fe level in umbilical cord blood, the highest tertile was associated with an increased risk of CHDs, especially for multiple CHDs, septal defects and patent ductus arteriosus. Overall, our study suggests a significant association between pregnancy Al exposure (≥ 2.408 mg/L) and risk of CHDs, especially for septal defects, and high Al and Fe elicited a strong correlation on fetal heart development. Future studies are needed to elucidate the underlying mechanism.
Investigation of high-risk HPV infection in high-grade lesions of the cervix and cervical cancer
Authors: 席 敏 , Li Xiaoduo , Sun Guang , Zhang Hailong , Xie Nanzi , Zhou Qin , Xu Bin , Bao Lunmin , Hu Xiaopin , Jiang Hongmei , Zhang Yundong 
Published: September 2022

Abstract
Background
By understanding the diagnostic value of high-risk HPV (HR-HPV) types in high-grade cervical lesions and cervical cancer, we expect to provide a theoretical basis for more targeted cervical lesion diagnosis and vaccine coverage and nucleotide polymorphism in Anshun, Guizhou Province.
Methods
10,774 samples were collected from people who underwent HPV typing test during June 2020 to December 2021 in Anshun People’s Hospital, Guizhou Province. Sources included outpatients, inpatients and physical examination participants. Main types of infection and the extent of cervical lesions were analyzed by PCR-reverse dot blot (PCR-RDB), TCT tests, colposcopy, pathological sections and histochemical staining. Meanwhile, single nucleotide polymorphisms (SNP) of HPV52 and HPV58 were analyzed and phylogenetic trees of E6/E7 regions of HPV52 and HPV58 were established by MEGA11.
Results
In Anshun area of Guizhou province, single infection dominated in HR-HPV infections, and the positive rate was 20.36%. The main types were HPV52 (6.11%), HPV16 (3.59%), HPV58 (3.04%), HPV53 (2.31%), and HPV51 (1.95%). Combined TCT test, colposcopy, pathological section and histochemical staining revealed that Type 16, 18, 52 and 58 could cause cervical lesions.The results of pathological section revealed that the positive rates were 27.94% and 36.36% for Type 52 and 58, respectively, which were higher than that of Type 16 and 18. SNP and evolutionary tree analysis revealed that in HPV52, there were 7 single nucleotide mutation sites in E6 and 3 in E7; among them, new mutations were found which included A125T, A294Tand T416C in E6, and T666C in E7, and all HPV52 subtypes were distributed in the B lineage.Similarly, in HPV58, there were 3 single nucleotide mutations found in E6 and 8 found in E7, which were exclusively distributed in the A lineage.
Conclusions
The prevalence of HR-HPV in Anshun area of Guizhou Province was consistent with that in the whole country, which mainly included Type 16, 18, 52, and 58, but with new mutation sites.