bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Vacuolar Transporter OsNRAMP2 Mediates Fe Remobilization During Germination and Affects Cd Distribution to Rice Grain
Authors: Chang Jia-Dong , Xie Yun , Zhang Huanhuan , Zhang Shurui , Zhao Fangjie 
Published: January 2022

Abstract
Background and aims Iron (Fe) deficiency in plants is a common problem affecting agricultural production. Cadmium (Cd) is a toxic metal that can be taken up and transported within plants by transporters for divalent metals including Fe(II). The present study aims to investigate the functions of OsNRAMP2 (Natural Resistance-Associated Macrophage Protein 2) in the remobilization and distribution of Fe and Cd in rice. Methods The expression pattern of OsNRAMP2 was determined by quantitative real-time PCR and pOsNRAMP2:GUS assay. Knockout mutants of OsNRAMP2 were generated by using CRISPR/Cas9 gene editing. Localization of Fe in the vacuolar globoids of germinating seeds was imaged by high-resolution transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy. Distributions of Fe and Cd between different plant tissues were investigated in hydroponic and soil pot experiments. Results OsNRAMP2 was mainly expressed in the embryo of germinating seeds, roots, leaf sheaths and leaf blades. OsNRAMP2 was localized at the tonoplast. Knockout of OsNRAMP2 delayed seed germination and produced chlorotic seedling leaves. Remobilization of Fe stored in the protein storage vacuoles in the scutellum of germinating seeds was restricted in osnramp2 mutants compared with wild type. Expression of genes related to Fe uptake was enhanced in the seedlings of osnramp2 mutants. Knockout of OsNRAMP2 significantly decreased the distribution of Cd, but not Fe, from leaves and straws to rice grains. Conclusions OsNRAMP2 plays an important role in remobilizing vacuolar Fe during seed germination and affects translocation of Cd from vegetative tissues to rice grains.
WITHDRAWN: Relative Frequency of Islet Autoimmunity in Children and Adolescents with Autoimmune Thyroid Disease
Authors: Putarek Natasa Rojnic , Krnic Nevena , Knezevic-Cuca Jadranka , Kusec Vesna , Baretic Maja , Dumic Miroslav 
Published: January 2022

Abstract
The present study aims to investigate islet autoimmunity and susceptibility to type 1 diabetes (T1D) in children/adolescents with autoimmune thyroid disease (AITD), and family members of AITD patients with islet autoimmunity. Islet-cell cytoplasmic, glutamic-acid decarboxylase and tyrosine-phosphatase autoantibodies were measured in 161 AITD patients [(127 with autoimmune thyroiditis (AT); 34 with Graves’ disease (GD)], 20 family members of AITD patients with islet autoimmunity, and 155 age-matched controls. Islet autoimmunity was found in 10.6% of AITD patients, significantly more frequent than in controls (1.9%; p=0.002). Higher prevalence of islet autoantibodies was found in females with AITD (p=0.011) but not in males (p=0.16) as well as in AT (p=0.013) but not GD patients (p=0.19), compared to corresponding controls. Two or three islet autoantibodies were found concurrently in six AITD patients with islet autoimmunity. They all developed T1D and had significantly higher islet autoantibodies titers (p=0.01) compared to AITD patients with single islet autoantibodies but normal glucose metabolism. T1D was found in 3.7% of AITD patients compared to 0.2% in age-matched, general Croatian population. Islet autoantibodies were found in 5/20 family members of AITD patients with islet autoimmunity, among which two developed T1D. None of the controls was positive to more than one islet autoantibodies or developed T1D. Conclusion: Children/adolescents with AITD (particularly females and patients with AT) represent a risk group for islet autoimmunity and T1D, as well as family members of AITD patients with positive islet autoantibodies, but last observation must be examined in a larger number of patients.
Storage Method of Multi-Channel Lidar Data Based on Tree Structure
Authors: Chen Hao , Gao Fei , Zhu Qingsong , Yan Qing , Hua Dengxin , Stanič Samo 
Published: January 2022

Abstract
The multi-channel lidar has the characteristics of fast acquisition speed, large data volume, high dimension, and strong real-time storage, which makes it difficult to be met using the traditional lidar data storage methods. This paper presents a novel approach to store and convert the multi-channel lidar data by traversal method of the tree structure and binary code. In the proposed approach, a tree structure is constructed based on the multi-dimensional characteristics of multi-channel lidar data and the hierarchical relationship between them. The adjacency table storage structure data in the memory is used to generate the sub-tree of the multi-channel lidar data. The results show that the proposed tree structure approach can save the storage capacity and improve the retrieval speed, which can meet the needs of efficient storage and retrieval of multi-channel lidar data.
Critical Appraisal of Minimally Invasive Keyhole Surgery for Intracranial Meningioma
Authors: Thakur Jai Deep , Mallari Regin Jay , Corlin Alex , Yawitz Samantha , Eisenberg Amalia , Rhee John , Sivakumar Walavan , Krauss Howard , Martin Neil , Griffiths Chester , Barkhoudarian Garni , Kelly Daniel F. 
Published: January 2022

Abstract
Purpose

Minimally invasive meningioma removal through transcranial and endoscopic endonasal keyhole routes remain controversial. Herein we detail results of keyhole meningioma removal defined as use of a minimally invasive “retractorless” approach for which a traditional larger approach is often used instead.
Methods

Retrospective analysis from 2008-2021 of consecutive patients undergoing keyhole meningioma removal through one of six approaches: extent of resection, complications, endoscopy use, MRI FLAIR/T2 changes. Surgical goal was maximal safe removal including conservative(subtotal) removal for some invasive locations.
Results

Of 329 patients, keyhole approaches were utilized in 193(59%) patients (mean age 59±13; 30(15.5%) prior surgery) who underwent 213 operations; 205(96%) were skull base location. Approaches included: endoscopic endonasal(n=74,35%), supraorbital(n=73,34%), retromastoid(n=38,18%), mini-pterional(n=20,9%), suboccipital(n=4,2%), and contralateral transfalcine(n=4,2%). Primary outcomes: Gross total/near total(>90%) resection: 125(59%); (5% for petroclival, cavernous sinus/Meckel’s cave, spheno-cavernous locations vs 77% other locations). Complications included: permanent neurological worsening12(6%); CSF leak 2(1%); meningitis 2(1%); no patients sustained DVT, PE, MI, or 30-day mortality. Median LOS was 3 days with 94% discharged home and 96% with favorable 90-day KPS. Secondary outcomes: Small persistent FLAIR/T2 changes: 11(5.2%) patients. Endoscopy use: 87/139(63%) of craniotomies, facilitating additional tumor removal in 55%. Tumor progression was observed in 26(13%) patients(mean follow-up 42±36 months).
Conclusion

This analysis suggests keyhole meningioma removal can be associated with reasonable resection rates, low complication rates, short hospitalizations and high 90-day performance scores. Subtotal removal may be appropriate for invasive/adherent meningiomas to avoid complications. With careful patient selection and requisite experience, these approaches may be considered alternatives to traditional approaches.
Genital and Oral Microbiome and Behçet’s Disease Activity
Authors: Senusi Amal A. , Ogunkolade William , Sandionigi Anna , Fortune Farida 
Published: January 2022

Abstract
BackgroundThe aetiopathogeneses of Behçet’s Disease (BD) remains elusive with multifactorial genetic and epigenetic factors resulting in multisystemic disease. Oral and genital ulceration are common and influences disease outcome. We hypothesised that dysregulation of genital and oral microbial communities contributes to BD disease activity. 153 BD patients’ samples, 70 matched oral and genital (Female: Male, 58:12; mean age, 42±13.9: 39.3±10.3), 12 unmatched samples; 16s rRNA sequencing utilised and V1/V2 and V3/V4 regions analysed. BD outcomes: oral and genital ulcer severity and BD activity scores, Psychological and Social Well-being scales, Headache Impact Test-6 (HIT-6) were included. All the analyses were performed with R software. ResultsThe alpha and beta diversity had anatomical specificity, with significant differences between genital and oral samples; p values<0.05 irrespective of presence or absence of ulcers. Interestingly, in the genital area Bacteroidota were present (G_U: 29% - 10%) and (G_nU: 27% - 14%) compared to less than 1% oral area of V1/V2 and V3/V4. Proteobacteria were uniquely present with (O_U: 9%) and (O_nU: 12%) in oral, and less than 0.01% in genital area for V3/V4 region. Gender anatomical specific communities were noted: females with genital ulcers Gardnerella, Lactobacillus, Atopobium were significantly increased compared to than males, with V3/V4 analysis indicating that Lactobacillus and Gardnerella were significantly increased by 20 times in females than males (p-adj <0.05). In contrast Peptoniphilus and Corynebacterium were significantly increased in males than females. Streptococcus was significantly increased with oral ulceration, while Veillonella was significantly decreased in patients without oral ulceration. Colchicine had a significant effect on the bacterial abundance irrespective of the presence or absence of ulceration. In this cohort, the WSAS (Work and Social Adjustment Scale) values were higher in active disease. ConclusionOur results suggest that dysregulated microbial communities occur in BD. V1/V2 demonstrates that during episodes of ulceration the pathogenic bacteria genus Escherichia-Shigella appear in both oral and genital ulcers. V3/V4 outcomes show that ulceration in both regions is assigned to genus; Lachnospiraceae, Saccharimonidales, Coriobacteriales. Streptococcus is related to the presence of oral ulcers, while Veillonella is presence when patients are ulcers free may be a useful marker of disease regression.
Crossed Andreev Reflection in Zigzag Phosphorene Nanoribbon Based Ferromagnet/Superconductor/Ferromagnet Junctions
Authors: Li Ruigang , Chen Lei , Liu Jun-Feng , Wang Jun 
Published: January 2022

Abstract
We study the crossed Andreev reflection in zigzag phosphorene nanoribbon based ferromagnet/superconductor/ferromagnet junction. Only edge states, which are entirely detached from the bulk gap, involved in the transport process. The perfect crossed Andreev reflection, with the maximal nonlocal conductance −2e 2 /h, is addressed by setting the chemical potentials of the leads properly. At this situation, the local Andreev reflection and the electron tunneling are completely eliminated, the incoming electrons can only be reflected as electrons or transmitted as holes, corresponding to the electron reflection and the crossed Andreev reflection respectively. The nonlocal conductance oscillates periodically with the length and the chemical potential of the superconductor. Our study shows that the phosphorene based junction can be used as the quantum device to generate entangled-electrons.
A Stochastic Model for Kala-azar Transmission Dynamics in Libo Kemkem, Ethiopia
Authors: Alemu Sewmehon Shimekaw 
Published: January 2022

Abstract
The objective of this paper is to analyse and demonstrate the dynamics of Kala-azar infected group using stochastic model, particularly using simple SIR model with python script over time. The model is used under a closed population with N = 100, transmission rate coefficient β = 0.09, recovery rate γ = 0.03 and initial condition I(0) = 1. In the paper it is discussed how the Kala-azar infected group behaves through simple SIR model. The paper is completed with stochastic SIR model simulation result and shows stochasticity of the dynamics of Kala-azar infected population over time. Fig. 2 below depicts continuous fluctuations which tells us the disease evolves with stochastic nature and shows random process.Subject: Infectious Disease, Global Health, Health Informatics and Statistical and Computational Physics
Safety and Immunogenicity of Mix-Match of Vaccines -Covishield and Covaxin – a Pilot Study
Authors: Attunuru Bhanu Prakash Reddy , Reddy Podduturi Naveenchander , Mitnala Sasikala , Deepika Gujjarlapudi , Veturi Sadhana Yelamanchili , Reddy Duvvur Nageshwar 
Published: January 2022

Abstract
This single-center prospective observational study was conducted to assess the safety and immunogenicity of combination vaccines AstraZeneca’s ChAdOx1-nCov-19 (Covishield in India) and inactivated whole virion BBV152 (Covaxin). A total of 330 unvaccinated healthy volunteers were screened for SARS-COV2 seropositivity. RT PCR tests were conducted for seronegative volunteers (n =44). They were randomly assigned to four groups and given either same or mixed vaccines at an interval of 4 weeks between the two doses. Mix and match of vaccines did not evoke any adverse events. Combination of vaccines elicited similar immune responses in 4 groups. They were further studied dividing into homologous and heterologous vaccine groups. In Conclusion, Combination vaccines are safe and immunogenic and heterologous vaccines elicit better immunogenic response.
Scyphocephalione a Isolated from the Stem Bark of Scyphocephalium Ochocoa (Myristicaceae) Attenuate Acute and Chronic Pains Through the Antiinflammatory Activity
Authors: Mbiantcha Marius , Feuya Raymond Tchouya Guy , Yousseu William Nana , Atsamo Donatien Albert , Foundikou Hibrahim , Lebibi Jacques , Zemo Franklin Gamo 
Published: January 2022

Abstract
Abstract

In the treatment of cancer, patients that receive anti-cancer drugs such as Vincristine develop peripheral neuropathic pain. Scyphocephalione A is a new bioactive compound isolated from Scyphocephalium ochocoa (Myristicaceae), a medicinal plant traditionally used in African countries. Recently, an in vitro study has shown its anti-inflammatory and cytotoxic activities on MCF-7 cell line of mammary carcinoma. The purpose of the present study was to assess the in vitro anti-inflammatory and in vivo anti-nociceptive activities of Scyphocephalione A. In vitro tests were carried out on cyclooxygenase and 5-lipoxygenase activities, and on protein denaturation; while in vivo tests were performed on acute and chronic pain models. It was noticed that, Scyphocephalione A (1000 µg/ml), inhibits proteins denaturation, cyclooxygenase and 5-lipoxygenase activities respectively by 74.21%, 75.80% and 64.43%. The dose 50 mg/kg of Scyphocephalione A, inhibits acetic acid (63.43%, p<0.001) and formalin (42.12% (p<0.001) within first phase and 67.53% (p<0.001) within second phase)-induced pains. At the same dose, Scyphocephalione A significantly inhibited mechanical and heat hyperalgesia, as well as cold allodynia induced by vincristine. In addition, the compound restored haematological, biochemical and oxidative stress parameters which were altered following Vincristine administration. These results suggest that Scyphocephalione A is endowed with anti-inflammatory potential and antinociceptive properties. Therefore, Scyphocephalione A can be classified as a promising molecule for the management of peripheral neuropathic pain triggered by anti-cancer drug.
A Novel Approach to Charcoal Fine Waste: Sustainable Use as Filling of Polymeric Matrices
Authors: Delatorre Fabíola Martins , Cupertino Gabriela Fontes Mayrinck , Oliveira Michel Picanço , Gomes Felipe Silva , Profeti Luciene Paula Roberto , Profeti Demetrius , Júnior Mário Guimarães , Azevedo Márcia Giardinieri , Saloni Daniel , Dias Ananias Francisco 
Published: January 2022

Abstract
This study aims to evaluate charcoal fines as potential reinforcing agents in biocomposites. Charcoal has both high carbon content and surface area depending on the manufacturing temperatures. Charcoal is a common residue in the coal industry that we propose using it to reinforce filling agents in several matrices in order to add value to this residue. This study investigated charcoal fines when using three pyrolysis temperatures (400, 600, and 800°C) to identify the most suitable charcoal to be used as raw materials in producing carbon biocomposites. We evaluated apparent density, porosity, morphology, and immediate chemical composition, and then performed a Fourier-transform infrared spectroscopy (FTIR) and an X-ray photoelectron spectroscopy (XPS). Charcoal fines produced at 800°C showed promising results as a polymeric matrix filling due to their higher porosity (81.08%), fixed carbon content (96.77%), and hydrophobicity.
Health-Related Quality Of Life And Clinical Outcome After Radiotherapy of Patients With Intracranial Meningioma
Authors: Lisowski Dominik , Trömel Jannik , Lutyj Paul , Lewitzki Victor , Hartrampf Philipp E. , Polat Bülent , Flentje Michael , Tamihardja Jörg 
Published: January 2022

Abstract
Purpose: This retrospective, single-institutional study investigated long-term outcome, toxicity and health-related quality of life (HRQOL) in meningioma patients after radiotherapy. Methods: We analyzed the data of 119 patients who received radiotherapy at our department from 1997 to 2014 for intracranial WHO grade I-III meningioma. Fractionated stereotactic radiotherapy (FSRT), intensity modulated radiotherapy (IMRT) or radiosurgery radiation was applied using a linear accelerator. A median total dose of 54.7 Gy (IQR 54.0-60.0 Gy) was prescribed in median single doses of 1.8 Gy (IQR 1.8-1.9 Gy). Overall survival (OS) and local control (LC) rates were obtained. In addition, the European Organisation for Research and Treatment of Cancer (EORTC) Quality of Life Core questionnaire (QLQ-C30) and the EORTC Brain Cancer Module (QLQ-BN20) were completed for assessment of HRQOL. Results were compared with normal population and with already published data. Results: OS for the entire cohort was 89.6% at 5 years and 75.9% at 10 years. LC at 5 and 10 years was 82.4% and 73.4%, respectively. Local recurrence was observed in 22 patients (18.5%). CTCAE grade ≥3 acute and chronic toxicities were observed in seven patients (5.9%) and five patients (4.2%), respectively. Global health status was rated with a mean of 59.9 points (SD 22.3) on QLQ-C30. HRQOL was lower after radiotherapy in comparison to normal population and to meningioma patients treated by surgery alone. Conclusion: Radiotherapy resulted in very good long-term survival and tumor control rates with low rates of severe toxicities. Nevertheless, long-term HRQOL may be negatively affected by radiotherapy.
Longitudinal Follow-Up on the Growth Rates and Influence Factors of Preterm and Full-Term Infants in Urban Areas of Hubei Province: A Birth Cohort Study
Authors: Xiong Zhonggui , Sun Feimin , Xia Zeyuan 
Published: January 2022

Abstract
Objective: It was to explore the growth rates and influence factors of length and weight of preterm and full-term infants in urban areas of Hubei province to provide reference for child health and related clinical field.Methods: A birth cohort study was used to analyze the growth rates and influence factors of length and weight of preterm and full-term infants in urban areas of Hubei province by multivariate regression analysis.Results: The growth rates of length and weight of preterm infants were significantly lower than those of full-term infants from birth to 3 months (P < 0.05), and gradually caught up to the level of full-term infants after 3 months (P > 0.05). Meanwhile, there were some influence factors which had significant differences by the contribution to the growth rates of length and weight of preterm and full-term infants. It was proposed that the perinatal factors were attached more importance to the growth rates of preterm infants.Conclusion: This study was to investigate the growth rates and influence factors of length and weight of preterm and full-term infants, and to take effective intervention measures to promote growth and development of preterm infants in urban areas of Hubei province.
Intron Retention Coupled with Nonsense-Mediated Decay is Involved in Cellulase Biosynthesis in Cellulolytic Fungi
Authors: Gao Yichen , Pang Ai-Ping , Ma Leyao , Wang Haiyan , Durrani Samran , Li Bingzhi , Wu Fu-Gen , Lin Fengming 
Published: January 2022

Abstract
Background

Knowledge on regulatory networks associated with cellulase biosynthesis is prerequisite for exploitation of such regulatory systems in ehancing cellulase production with low cost. The biological functions of intron retention (IR) and nonsense-mediated mRNA decay (NMD) in filamentous fungi is lack of study, let alone their roles in cellulase biosynthesis.
Result

We found that major cellulase genes (cel7a, cel7b, and cel3a) exhibited concomitant decrease in IR rates and increase in their gene expression in T. reesei under cellulase-producing condition (cellulose and lactose) that was accompanied with a more active NMD pathway, as compared to non cellulase-producing condition (glucose). In the presence of the NMD pathway inhibitor that successfully repressed the NMD pathway, the mRNA levels of cellulase genes were sharply down-regulated, but the rates of IR in these genes were significantly up-regulated. Consistently, the cellulase activities were severely inhibited. In addition, the NMD pathway inhibitor caused the downregulated mRNA levels of two important genes of the target of rapamycin (TOR) pathway, trfkbp12 and trTOR1. The absence of gene trfkbp12 made the cellulase production in T. reesei more sensitive to the NMD pathway inhibitor.
Conclusion

All these findings suggest that the IR of cellulase genes regulates their own gene expression by coupling with the NMD pathway, which might involve the TOR pathway. Our results provide better understanding on intron retention, the NMD pathway, and cellulase production mechanism in filamentous fungi.
Combinatorial Design of the MAUT and PAMSSEM II Methods for Multiple Attributes Group Decision Making with Probabilistic Linguistic Information
Authors: Kong Qiaoyu , Wu Liangping 
Published: January 2022

Abstract
This paper considers the application of probabilistic linguistic term sets (PLTS) in multiple-attribute group decision-making (MAGDM) when the weights can’t be determined. First, as an improvement of the PROMETHEE method, the PAMSSEM method can not only handle missing evaluations, but also proposes a rejection threshold to calculate the overall consistency of the plan, so as to rank the plan more reasonably. At the same time, the MAUT uses the marginal utility function to reallocate the attribute values of the alternatives in the interval , and then calculate the total utility to sort them. Because the utility function is beneficial in expressing consumer satisfaction, we combine the MAUT method and PAMSSEM II method and apply it to solve decision-making problems under probabilistic linguistic environment. Secondly the coefficient of variation method, entropy method and analytic hierarchy process are used to calculate the weights in a combination. In the process of data processing, we use the transfer function to convert the PLTS into the hesitant probabilistic fuzzy set (HPFS) for calculation. Finally, the PL-MAUT-PAMSSEM II method, PROMRTHE method, TOPSIS method and ARAS method are compared with each other.