bluuebunny/crossref_metadata_2025_split huggingface dataset:
Road traffic density and recurrent asthma emergency department visits among Medicaid enrollees in New York State 2005-2015
Authors: Insaf Tabassum Z , Adeyeye Temilayo , Wargo Catherine , Wagner Victoria , Proj Anisa , McCauley Susan , Matson Jacqueline 
Published: January 2022

Abstract
Background

Environmental exposures such as traffic may contribute to asthma morbidity including recurrent emergency department (ED) visits. However, these associations are often confounded by socioeconomic status and health care access.
Objective

This study aims to assess the association between traffic density and recurrence of asthma ED visits in the New York State (NYS) Medicaid population between 2005 and 2015.
Methods

The primary outcome of interest was a recurrent asthma ED visit within 1-year of index visit. Traffic densities (weighted for truck traffic) were spatially linked based on home addresses. Bivariate and multivariate logistic regression analyses were conducted to identify factors predicting recurrent asthma ED visits.
Results

In a multivariate model, Medicaid recipients living within 300-meters of a high traffic density area were at a statistically significant risk of a recurrent asthma ED visit compared to those in a low traffic density area (OR=1.31; 95% CI:1.24 – 1.38). Additionally, being male (OR=1.24; 95% CI:1.20 – 1.27), being on cash assistance (OR=1.27; 95% CI:1.24 – 1.31) and receiving supplemental security income (OR=1.23; 95% CI:1.18 – 1.27) was associated with a higher risk of recurrent asthma ED visit. Black non-Hispanics (OR=1.20; 95% CI:1.10 – 1.31), Hispanics (OR=1.52; 95% CI:1.46 – 1.59) and those with race listed as “Other” (OR=1.58; 95% CI:1.48 – 1.68) had higher risk of recurrent asthma ED visits as compared to White non-Hispanics.
Conclusion

We observed significant persistent disparities in asthma morbidity related to traffic exposure and race/ethnicity in a low-income population. Our findings suggest that even within a primarily low-income study population, socioeconomic differences persist. These differences in susceptibility in the extremely low-income group may not be apparent in health studies that use being on Medicaid as a proxy for low income
Effects of process parameters and geometry on dimensional accuracy and surface quality of thin strut heart valve frames manufactured by laser powder bed fusion
Authors: Zhao Xiao , Liang Anqi , Bellin Matteo , Bressloff Neil W. 
Published: January 2022

Abstract

Laser powder bed fusion (LPBF) is one of the most popular metal additive manufacturing technologies, which has found its applications in high-value sectors such as aerospace and biomedical devices. Some recent studies on the LPBF of stents have demonstrated its feasibility in the fabrication in thin strut structures including heart valve frames, as used in transcatheter aortic valve implantation (TAVI) for the treatment of severe aortic stenosis. The state of the art method of laser cutting TAVI frame limits the scope for novel concepts which are made possible by additive manufacturing. However, the surface quality and dimensional accuracy of LPBF parts are lower than that produced by laser cutting. To start the development of new TAVI concepts, the feasibility of manufacturing thin frames by LPBF has been investigated based the SAPIEN 3 frame by Edwards Lifesciences. In this study, simplified frames with strut size from 0.3 mm to 0.5 mm have been successfully manufactured. The effects of strut size, strut angle, laser power and scan speed on the dimensional accuracy and surface quality were systemically studied. In addition, a representative SAPIEN 3 frame was manufactured and assessed with high resolution X-ray scans. Good overall dimensional accuracy and low porosity was obtained for the SAPIEN 3 frame. However, inclined struts were found to have relatively low dimensional accuracy and poor surface quality.
Nitrogen Use Efficiency of Early Open-Pollinated Maize Varieties and their F1 Hybrids for Tolerance to Low Soil Nitrogen Stress
Authors: Ige S. A , Bello O. B. , Mahamood J. , Afolabi M 
Published: January 2022

Abstract
In the tropical savanna of Africa where low soil nitrogen (N) is a major constraint to maize production, the development of early maturing maize tolerance to low-N and drought is very essential. Field trials were conducted at the Lower Niger River Basin Development Authority, Ilorin, Nigeria on a Typic Paleustalf soil to assess the response of ten early drought-tolerant open-pollinated varieties (OPVs) and their F1 hybrids to low-N fertilizer conditions. The trials were set up in a split-plot arrangement with three N fertilizer levels (0, 45 and, 90 kg N ha−1) as the main plot and the genotypes as sub-plot. Each sub-plot within N level was four-row, laid out in a randomized complete block design of four replications. Planting was carried out on 22nd July, 2018 and 20th July, 2019. There were significant interactions between the genotypes and N level for all characters, with increased variation as the level of N decreased. The maize grain yield advantage was 1.72 t ha−1 and 1.95 t ha−1 for OPVs and hybrids, respectively; with applied 90 kg N ha−1 over no N. Nitrogen use efficiency (NUE) was optimum at 45 kg N ha−1 in all the genotypes. Grain yield and NUE correlated positively with all the traits measured except days to 50% silking. Kernel number was most severely reduced by N stress but had a positive and highly significant correlation with grain yield at all N-rates. The hybrids Dmr-Esr Y Cif 2 × Ak 95 Dmr-Esrw, Ev Dt-Y 2000 Str C0 × Ak 95 Dmr-Esrw, and Tze Comp 4 C2 × Tze Comp 3 C2 had superior grain yield and NUE. This suggests that selecting for tolerance to drought may simultaneously confer tolerance to low-N stress.
Identification of a novel splicing mutation and genotype-phenotype correlations in rare PLS3-related osteoporosis
Authors: Wu Zhichong , Feng Zhenhua , Zhu Xiufen , Dai Zhicheng , Min Kaixing , Qiu Yong , Yi Long , Xu Leilei , Zhu Zezhang 
Published: January 2022

Abstract
Background

X-linked early-onset osteoporosis, caused by mutations in plastin3 (PLS3), is an extremely rare disease characterized by low bone mineral density (BMD) and recurrent osteoporotic fractures. There is limited information on genetic and phenotypic spectrum, as well as genotype-phenotype correlations of the disease. Moreover, whether decreased PLS3 levels were also involved in osteoporosis among subjects without PLS3 pathogenic mutations remains unknown.
Results

We identified a novel splicing mutation (c.892-2A>G) which led to the skipping of exon 9 in a family with X-linked early-onset osteoporosis. Scoliosis represents a new phenotype in the patients harboring PLS3 mutations, which may be corrected by brace treatment. Genotype-phenotype analysis reveals that there was no significant difference in BMD z-scores between different types of reported mutations including this study (p = 0.5). There is a marginally significant negative correlation between age and BMD z-score (p = 0.059, r = - 0.30). The conditions of osteoporosis in all patients were improved after bisphosphonates therapy, with mean BMD z-score increased from -2.9 to -0.57 (p < 0.0001). Serum PLS3 levels in adolescents and adults without PLS3 pathogenic mutations but representing osteoporosis were also evaluated, while no association was found between bone mineral density and PLS3 levels (p > 0.05).
Conclusions

Our findings expanded the mutation and phenotype spectrum of the rare disease and highlights the importance of early diagnosis and early treatment with bisphosphonates. More reports of cases with PLS3 mutation and function studies of the gene are warranted to understand genotype-phenotype correlations.
Colorectal Cancer Metastases In The Reproductive Tract – A Systematic Review
Authors: Alfred Joshua , Wyatt James , Anuar Amir , Williams Ruby , Titley-Wilson Gabrielle , Ahmed Shakil , Altaf Kiran 
Published: January 2022

Abstract
BACKGROUND: Colorectal cancer metastases are common and usually affect the liver, lungs, peritoneum or bone. However, unusual cases of metastatic disease within the reproductive tracts of both male and female patients have been described. This review aims to collate and summarise the available data to aid clinical decision making and patient counselling. METHODS: Pubmed, MEDLINE and the Cochrane databases were searched from inception till November 2020 for case reports and cases series describing original data on colorectal cancer metastases within the reproductive tract. RESULTS: 29,216 results were filtered down to 89 studies for inclusion. 43 and 46 related to the male and female reproductive tracts respectively, with individual patient outcomes reported for 48 males and 461 females. Genital metastases were associated more with rectal cancers whilst ovarian and testicular tumours more commonly derived from colonic primaries. Treatment was with curative intent for the minority of male patients (32%) with an associated poor prognosis (4% 5-year overall survival). Female reproductive tract metastases with ovarian lesions in particular were more likely to receive curative intent treatment (63%) and superior long-term survival (17% 5-year overall survival)CONCLUSIONS: Reproductive system colorectal cancer metastases are rare with limited cases reported. They are associated with advanced primary tumours and disseminated disease with an associated poor prognosis.
Sex and APOE Genotype Alter the Basal and Induced Inflammatory State of Primary Microglia from APOE Targeted Replacement Mice
Authors: Mhatre-Winters Isha , Eid Aseel , Han Yoonhee , Tieu Kim , Richardson Jason R. 
Published: January 2022

Abstract
Background: Sex and APOE4 genotype are significant risk factors for Alzheimer’s disease (AD), with females demonstrating increased risk that is modulated by APOE genotype. However, the mechanism(s) responsible for this interaction are still a matter of debate. Neuroinflammation represents a plausible mechanism for this interaction, but most experimental studies have not incorporated both sex and APOE genotype. Here, we sought to assess the response of sex-specific APOE3 and APOE4 primary microglia (PMG) in comparison to traditional mixed sex cultures. Methods: Mixed sex and sex-specific PMG were generated from neonatal targeted replacement APOE3 and APOE4 pups. Purity over 98% was consistently obtained for cultures and the presence or absence of the Sry (Sex-determining region Y) gene by qPCR was used to determine male-specific microglia. PMG were treated with 10 ng/mL or a combination of lipopolysaccharide (LPS) and interferon-gamma (IFNg). Pro- and anti-inflammatory cytokine profiles were assessed by quantitative RT-PCR and multiplex MSD assays.Results: Mixed sex APOE4 PMG were found to have higher basal mRNA expression of pro-inflammatory cytokines including TNFa, and iNOS compared to APOE3 PMG which was accompanied by increased levels of secreted TNFa, but not nitric oxide. In sex-specific cultures, basal expression of IL1b, TNFa, IL6, and iNOS was 2-3 fold higher in APOE4 female PMG compared to APOE4 males, with both being higher compared to APOE3 cells. Similar results were found for protein levels of these cytokines. Following an inflammatory stimulus, the expression of pro-inflammatory cytokines was upregulated in the order E4 female > E4 male > E3 female > E3 male in sex-specific cultures. Similar results were found with secreted cytokine levels.Conclusions: These data indicate that the APOE4 genotype and female sex, together contribute to a greater inflammatory response in PMG isolated from targeted replacement APOE mice. These data are consistent with clinical data and sex-specific PMG may provide a platform for exploring mechanisms of genotype and sex differences in AD-related to neuroinflammation and neurodegeneration.
Incidence of Graft Detachment Compared Between Two Techniques of Ultrathin Descemet’s Stripping Automated Endothelial Keratoplasty: A Case Series.
Authors: Feuerman Oren Mark , Caretti Luigi , Agresta Antonio , Monterosso Cristina , Verzola Giacomo 
Published: January 2022

Abstract
Purpose
To evaluate the incidence of rebubbling for graft detachment in two different surgical techniques and postoperative management of Ultrathin Descemet’s Stripping Automated Endothelial Keratoplasty (UT-DSAEK).
Methods
A Longitudinal retrospective and prospective case series of 50 eyes operated between November 1st, 2017 and November 1st, 2020. Graft detachment and consequent rebubbling incidence were registered and reviewed in two groups of consecutive patients with Fuchs endothelial dystrophy. The the two surgical techniques differed in diameter of Descemetorhexis and presence or absence of preoperative inferior Nd:YAG laser iridotomy. Main outcome measures were incidence of rebubbling in 30 days postoperative period and incidence of ocular hypertention.
Results
Incidence of graft detachment and consequent rebubbling procedure are lower among eyes who underwent iridotomy and had a larger diameter Descemetorhexis than graft diameter. Intraocular pressure resulted higher among patients who did not undergo preoperative inferior Nd:YAG laser iridotomy.
Conclusions
Preoperative lower iridotomy and less postoperative eye manipulation are associated with less graft dislocation following Ultrathin Descemet’s Stripping Automated Endothelial Keratoplasty.
m6A-mediated upregulation of LINC01578 promotes the progression of glioma by modulating the miR-6893-3p/TRIM14 axis
Authors: Wu Xinwei , Yang Hui , Zhu Xiaolong , Zhong Min , Zhang Mengying , Lv Kun 
Published: January 2022

Abstract
Background
Long noncoding RNAs (lncRNAs) play crucial roles in tumor progression and are dysregulated in glioma. However, the functional roles of lncRNAs in glioma remain largely unknown.
Methods
Using the TCGA and GEPIA2 databases, the overexpression of LINC01578 in glioma tissues was determined and further validated in glioma cell lines. The effects of LINC01578 on proliferation, migration, and invasion in glioma were detected by in vitro and in vivo experiments. RNA immunoprecipitation (RIP), dual luciferase reporter, RNA FISH, and RNA sequencing assays were carried out to elucidate the underlying molecular mechanisms regulated by LINC01578 in glioma. RIP and RT-qPCR was used to analyze the regulatory effect of N6-methyladenosine (m6A) on aberrantly expressed LINC01578.
Results
Highly expressed LINC01578 was identified in glioma tissues and was associated with poor prognosis in glioma patients. Functional assays showed that LINC01578 regulates glioma growth and metastasis in vitro and in vivo. Mechanistically, LINC01578 sponged miR-6893-3p to upregulate TRIM14 expression, thereby facilitating glioma progression. Furthermore, LINC01578 was upregulated in response to N6-methyladenosine modification, which was attributed to METTL3/YTHDF1-mediated RNA transcripts.
Conclusion
Our results reveal a novel m6A/LINC01578/miR-6893-3p/TRIM14 pathway for glioma progression and suggest LINC01578 as a novel prognostic indicator and therapeutic target for glioma.
Ultra-Low dose contrast using Transluminal Renal Angioplasty (ULTRA study)
Authors: Kawano Megumi , Suzuki Kenji , Fujimura Naoki , Endo Ayaka , Kato Ai , Fujii Kentaro , Komatsu Motoaki , Takahashi Toshiyuki , Ryuzaki Munekazu , Harada Hirohisa 
Published: January 2022

Abstract
BackgroundSevere atherosclerotic renal artery stenosis (ARAS) is a predictor of future cardiovascular events and should be managed effectively. This study aimed to validate the safety and efficacy of percutaneous transluminal renal angioplasty (PTRA) using low concentration digital subtraction angiography (LC-DSA) among patients with severe ARAS and advanced chronic kidney disease (CKD).MethodsThis prospective study conducted between August 2018 and August 2020. Patients with CKD stage 3b or worse and significant renal artery stenosis were included in this study and underwent PTRA with ultra-low dose contrast medium. The primary endpoint was a change in renal function, based on serum creatinine (sCr) levels.ResultsThirteen lesions of eleven patients underwent PTRA for ARAS with ultra-low dose contrast medium. Mean sCr level has significantly improved from 3.18 ± 1.8 mg/dL (before procedure) to 2.34 ± 1.17 mg/dL (one month after treatment) (p =0.039). The mean amount of used contrast was 8.8 ± 4.0 ml per vessel. Patients with renal artery stenosis >80%, peak systolic velocity >300 cm/m2, or CKD Stage 3b were analyzed. More severe stenosis and rapid deterioration of renal function before treatment were associated with improved kidney function. No cardiovascular and renal complications, such as stroke or contrast-induced nephropathy, were noted in any of the patients.ConclusionsPTRA with ultra-low dose contrast medium is safe and provides good results.Trial registration: Investigation for optimal protocol using diluted ionic contrast medium during angiography, UMIN000034174. Registered 26 September 2018, https://center6.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000038955
Evaluation of Expression CXCL8 Chemokine and Its Relationship With Oocyte Maturation and Embryo Quality in Intracytoplasmic Sperm Injection Method
Authors: Jenabi Maryam , Khodarahmi Parvin , Tafvizi Farzaneh , Bostanabad Saeed Zaker 
Published: January 2022

Abstract
BackgroundThe present study aimed to evaluate the expression of the chemokine CXCL8 in both mRNA and protein levels in the serum, follicular fluid (FF), and cumulus cells (CCs) and its relationship with oocyte maturation and embryo quality in women undergoing intracytoplasmic sperm injection (ICSI).MethodsA total of 87 women who underwent an ICSI cycle were evaluated in two groups, including the test group (female factor infertility) and the control group (fertile). In the serum, FF, and CCs, the protein and mRNA expression of CXCL8 were measured using immunosorbent assay and Real-Time PCR, respectively. The quality and quantity of the oocytes and embryos were assessed, and relationship of protein and mRNA CXCL8 was evaluated with oocyte maturation and embryo quality. ResultsThe level of protein and mRNA of CXCL8 was significantly higher in the serum, FF, and CCs in the test group than in the control group. In the test group, the expression of mRNA and protein of CXCL8 had a significant increase in FF and CCs compared to serum; also, there was a CXCL8 protein significant increase in FF compared to CCs. The count of oocytes obtained, MII oocytes, and the percentage of oocyte maturity significantly decreased in the test group. The expression of CXCL8 was inversely related to oocyte maturation, but no relationship was observed with embryo quality.ConclusionThe elevated concentrations of CXCL8 in the serum and FF seem to be a predictor as a potential non-invasive biomarker for the oocyte maturation outcome in women with female factor infertility.
Low-Carbohydrate Diets and Mortality in Older Chinese: A 15-Year Follow-Up of Guangzhou Biobank Cohort Study
Authors: Sun Ce , Zhang Wei Sen , Jiang Chao Qiang , Jin Ya Li , Deng Xue Qing , Woo Jean , Cheng Kar Keung , Lam Tai Hing , Thomas G Neil , Xu Lin 
Published: January 2022

Abstract
Background: Studies on long-term effects of low-carbohydrate diet (LCD) on mortality accounting for quality and sources of carbohydrate are unclear. Hence, we examined the associations of LCDs with all-cause and cause-specific mortality in a population-based cohort study.Methods: Prospective study using data from Guangzhou Biobank Cohort Study (GBCS). 20,206 participants (13.8% diabetes) aged 50+ years were recruited from 2003-2006 and followed up until 19 April 2021.Overall, vegetable-based, and meat-based LCD scores were calculated based on the percentage of energy as total and subtypes of carbohydrate, fat and protein. The main outcomes were all-cause, cancer and CVD mortality. Cox regression were used to calculate hazard ratios (HRs) and 95% confidence intervals (CIs).Results: During 294,848 person-years of follow-up, 4,624 deaths occurred, including 3,661 and 963 deaths in participants without and with diabetes, respectively. In all participants, overall LCD score was not associated with all-cause and cause-specific mortality after multivariable adjustment. However, the adjusted HRs (95%CIs) of all-cause and CVD mortality for the highest, versus the lowest, quartiles of vegetable-based were 1.16 (1.05-1.27 Ptrend<0.001) and 1.39 (1.19-1.62 Ptrend<0.001), respectively. Meanwhile, we found that the adjusted HRs (95%CIs) of all-cause and CVD mortality for highest, versus lowest, quartile of meat-based LCD were 0.89 (0.81-0.97 Ptrend=0.007) and 0.81 (0.70-0.93 Ptrend=0.02), respectively. Similar associations were found in participants after excluding those with diabetes. In diabetic patients, there was no association between vegetable-based LCD or meat-based LCD scores and mortality. However, we found a positive association between the highest quartile of vegetable-based LCD score and CVD mortality comparing with the lowest quartile (HR 1.54, 95% CI, 1.11-2.14 Ptrend=0.003).Conclusions: Vegetable-based LCD score was positively and meat-based LCD score was negatively associated with all-cause and CVD mortality. Inconsistencies in the literature on the health effect of LCD may reflect the importance of the geographical context and age-related nutrient composition of the diet.
Development of genomic instability-associated long non-coding RNA signature: a prognostic risk model of clear cell renal cell carcinoma
Authors: Jiang Hua , Chen Ming 
Published: January 2022

Abstract
Background: Renal cell carcinoma is one of the most common cancers in the urinary system. Renal clear cell carcinoma is the most lethal of all pathological subtypes. Genomic instability was recently reported to be relative with occurrence and development of renal cells. The biological roles of long non-coding RNAs (lncRNAs) in tumorigenesis have been increasingly valued, and various lncRNAs were found to be oncogenes or cancer suppressors. Here, we identified a novel genomic instability-associated lncRNAs (GILncs) model for ccRCC patients to predict overall survival (OS).Methods: The Cancer Genome Atlas database was applied to obtain full transcriptome data, somatic mutation profiles and clinical characteristics. The differential expressed lncRNAs between genome-unstable (GU) like group and genome-stable (GS) like group were defined as GILncs, with |logFC| >1 and adjusted p value< 0.05 of false discovery rate. All samples were allocated into GU-like type and GS-like type based on expression of GILncs using hierarchical cluster analyses. A genomic instability-associated lncRNA signature (GILncSig) was constructed using parameters of included lncRNAs. Quantitative real-time PCR analysis was used to detect the expression of included lncRNAs in vitro. Validation of the risk model was performed by Kaplan–Meier (K-M) log-rank test, Time-dependent receiver operating characteristic curve analysis and multivariate Cox regression analysis.Results: 46 lncRNAs were identified as GILncs. LINC00460, AL139351.1 and AC156455.1 were employed for GILncSig calculation based on the results of multivariate Cox analysis. GILncSig was confirmed as an independent index for predicting OS and concerned with malignant progression of ccRCC. Additionally, the GILncSig Score presented better efficiency and accuracy than two lncRNA-related models published before.Conclusion: GILncSig Score was qualified as a critical indicator independent from other clinical factors for predicting prognostic risk of ccRCC patients.