bluuebunny/crossref_metadata_2025_split huggingface dataset:
Herbal melanin modulates PGE2 and IL-6 gastroprotective markers through COX-2 and TLR4 signaling in the gastric cancer cell line AGS
Authors: El-Obeid Adila , Maashi Yahya , Alatar Ghada , Aljudayi Modhi , Al-Eidi Hamad , AlGaith Nouf , Khan Altaf Husain , Hassib Adil , Matou-Nasri Sabine 
Published: May 2022

Abstract
We reported a gastric anti-ulcerogenic effect of the Nigella sativa (L.)-derived herbal melanin (HM) in rat models. However, the molecular mechanisms underlying the HM gastroprotective effect remain unknown. Cyclooxygenase-2 (COX-2)-catalyzed prostaglandin E2 (PGE2) and toll-like receptor 4 (TLR4)-mediated interleukin-6 (IL-6) production and secretion play major roles in gastric mucosal protection. In the current study, we investigated the effect of HM on TLR4, COX-2, and on gastroprotective markers PGE2 and IL-6 expression, using the human gastric cancer cell line AGS. Bacterial lipopolysaccharide (LPS), a well-known inducer of gastroprotective marker expression, was used as a positive control. After cell exposure to HM and LPS, the cell lysates and supernatants were tested for COX-2, PGE2, and IL-6 production and secretion using Western blot technology and an enzyme-linked immunosorbent assay (ELISA), respectively. Immuno-cyto-fluorescence staining was applied for COX-2 cellular localization and RT-qPCR for monitoring the gene expression levels of TLR4, COX-2, and mucin 4, an important gastroprotective glycoprotein. In this study, we showed that HM upregulated its main receptor TLR4 gene and protein expression. HM increased, in a dose- and time-dependent manner, the secretion of PGE2 and the expression of COX-2 mRNA and protein, which was detected in the nucleus, cytoplasm and predominantly at the intercellular junctions. In addition, HM enhanced IL-6 production and secretion, and upregulated the mucin 4 gene expression, the hallmarks of gastroprotection. To check whether HM-induced PGE2 and IL-6 through TLR4 signaling and COX-2 generated, AGS cells were pre-treated with a TLR4 signaling inhibitor TAK242 and the COX-2 inhibitor NS-398. An inhibition of HM-induced COX-2, PGE2 and IL-6 production was observed in TAK242 and NS-398-pre-treated AGS cells, confirming the role of TLR4 signaling and of COX-2 generated in the possible HM gastroprotective effects. In conclusion, our results showed that HM enhances TLR4/COX-2-mediated secretion of gastroprotective markers PGE2 and IL-6, and upregulates mucin 4 gene expression in the human gastric cancer cell line AGS.
Comparative environmental RNA and DNA metabarcoding analysis for ecological surveys of river algae and arthropods
Authors: Miyata Kaede , Inoue Yasuaki , Amano Yuto , Nishioka Tohru , Nagaike Tomohisa , Kawaguchi Takamitsu , Morita Osamu , Yamane Masayuki , Honda Hiroshi 
Published: May 2022

Abstract
Environmental DNA (eDNA) metabarcoding is widely used for species analysis, while the use of environmental RNA (eRNA) metabarcoding is more limited. We conducted comparative eDNA/eRNA metabarcoding of the algae and arthropods (aquatic insects) in water samples from Naka River, Japan to evaluate their potential for biological monitoring and water quality assessment. Both methods detected various algae and arthropod species, however, their compositions were remarkably different from those in traditional field surveys (TFSs), indicating a low sensitivity. For algae, the species composition derived from eDNA and eRNA metabarcoding was equivalent. While TFSs focus on attached algae, metabarcoding analysis theoretically detects both planktonic and attached algae. A recently expanded genomic database for aquatic insects significantly contributed to the sensitivity and positive predictivity for arthropods. While the sensitivity of eRNA was lower than that of eDNA, the positive predictivity of eRNA was higher. The eRNA of terrestrial arthropods indicated extremely high or low read numbers when compared with eDNA, suggesting that eRNA could be an effective indicator of false positives. Arthropod eDNA/eRNA metabarcoding analysis enabled water quality estimates from TFSs. The eRNA of algae and arthropods could thus be used to evaluate biodiversity and water quality and provide insights from ecological surveys.
Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
Authors: Mon Maria Laura , de Villegas Rubén Marrero Díaz , Campos Eleonora , Soria Marcelo A. , Talia Paola M. 
Published: May 2022

Abstract
In this study we report the biochemical and molecular structural characterization of a novel alkali-thermostable GH10 xylanase (Xyl10B) identified in termite gut microbiome by a shotgun metagenomic approach. This endoxylanase candidate (Xyl10B) was amplified, cloned, heterologously expressed in Escherichia coli and purified. Xyl10B was active in a broad range of temperature (37–60 ºC) and pH (4–10) with optimum activity at 50 ºC and pH 9. Moreover, it activity remains at more than 80% of its maximum at 50°C for 8h. In addition, Xyl10B showed to be halotolerant and stable in the presence of several ions and chemical reagents frequently used in the industry. These characteristics makes this enzyme an interesting candidate in the paper bleaching of pulp and paper industries, since this industry requires enzymes with no cellulase activity and thermo-alkaliphilic to resist the high temperature and alkaline pH reached in the procedures. On the other hand, the xylan hydrolytic products (lower xylooligosaccharides, xylose and xylobiose) liberated by the action of Xyl10B could be suitable for application as prebiotic and in the production of bioethanol. Thus, the enzyme may be a useful candidate for other industrial application as well.
Intrathecal CAR-NK cells infusion for isolated CNS relapse after allogeneic stem cell transplantation
Authors: YUAN Jing , WANG Zhen-zhen , REN Han-yun , WANG Fu-xu 
Published: May 2022

Abstract
A 24-year-old man with central nervous system (CNS) involvement of T-cell lineage acute lymphoblastic leukemia received sibling allogeneic stem cell transplantation (allo-SCT). He developed isolated CNS relapse early post-SCT, while high-dose systemic chemotherapy, intrathecal (IT) triple infusion and IT donor lymphocytes infusion (DLI) all demonstrated effectiveness. We performed IT umbilical cord blood-derived CAR-NK cells infusion. After infusion, detection of cytokines revealed that interferon-γ, interleukin-6 and interleukin-8 increased in CSF. He developed high fever, headache, nausea, vomiting and a spinal cord transection with incontinence in a short time, whereas the ptosis and blurred vision improved completely. The bone marrow remained encouragingly complete remission and complete donor chimerism over 9 months after IT CAR-NK infusion. In conclusion, IT CAR-NK cells infusion is a potentially feasible and effective option for patients with CNS relapse, with limited neurological toxicity.
Accelerating Syngas-to-Aromatic Conversion via Spontaneously Monodispersed Fe in ZnCr2O4 Spinel
Authors: Tian Guo , Liu Xinyan , Zhang Chenxi , Fan Xiaoyu , Xiong Hao , Chen Xiao , Li Wenzheng , Yan Binhang , Zhang Lan , Wang Ning , Peng Hong-Jie , Wei Fei 
Published: May 2022

Abstract
Spontaneous monodispersion of Fe species and their stabilization in reactive atmospheres remain a key challenge in catalytic syngas chemistry. In this study, we present a catalyst with spontaneously monodispersed Fe on ZnCr2O4, which shows remarkable performance in the syngas-to-aromatic reaction when coupled with a H-ZSM-5 zeolite. Monodispersed Fe increases the turnover frequency from 0.54 to 2.48 h−1 without sacrificing the record high selectivity total aromatic (80%–90%) at a single pass. This is enabled by the activation of CO and H2 at oxygen vacancy nearest to the isolated Fe site and the prevention of carbide formation. Atomic precise characterization and theoretical calculations shed light on the origin and implications of spontaneous Fe monodispersion.
Optimal timing of laparoscopic cholecystectomy after conservative therapy for acute cholecystitis
Authors: Enami Yuta , Aoki Takeshi , Tomioka Kodai , Hakozaki Tomoki , Hirai Takahito , Shibata Hideki , Saito Kazuhiko , Nagaishi Shodai , Takano Yojiro , Seki Junichi , Shimada Shoji , Nakahara Kenta , Takehara Yusuke , Mukai Shumpei , Sawada Naruhiko , Ishida Fumio , Kudo Shin-ei 
Published: May 2022

Abstract
Background: Tokyo Guidelines 2018 recommend early surgery for acute cholecystitis. However, some elective surgery cases depend on complications or facility conditions. In this study, we retrospectively analyzed the cases of elective surgery.Methods: This study included 345 patients who underwent laparoscopic cholecystectomy (LC) at our hospital between January 2019 and December 2020. Eighty-three patients underwent LC four days or more after conservative treatment. The elective LC cases were divided into two groups: Early group (4-90 days after onset, n=36) and Delayed group (91+ days after onset, n=31). Percutaneous transhepatic gallbladder drainage cases (n=16) were excluded.Results: The operative times were significantly shorter in the Delayed group (91.2 min, p=0.0108) than the Early group (117 min). However, the amount of blood loss was not significantly different between the two groups. Furthermore, the length of hospital stay was shorter in the Delayed group (3.4 days, p=0.0436) than in the Early group (5.9 days).There were no significant differences in the incidence of complications or the rate of conversion; however, the rates of these two factors were reduced in the Delay group compared to the Early group.Conclusions: If it has become challenging to perform urgent/early LC within three days, due to prior conservative treatment for acute cholecystitis, the operative time and length of hospital stay could be reduced by delaying LC for more than 90 days after the onset. In addition, postoperative complications would not occur, and the conversion rate may be reduced to a low level.
Toxic effect of metal doping on diatoms as probed by Broadband Terahertz Time-Domain Spectroscopy
Authors: Kumar Rohit , Paturzo Melania , Sardo Angela , Orefice Ida , Qiucheng Yu , Rubano Andrea , Paparo Domenico 
Published: May 2022

Abstract
The global marine environment is increasingly affected by human activities, causing climate change, eutrophication, and pollution. These factors influence the metabolic mechanisms of phytoplankton species, such as diatoms. Among the other pollutant agents, heavy metals can have dramatic effects on diatom wellbeing. Therefore, a detailed knowledge of the interaction of diatoms with metals is essential from both a fundamental and applicative point of view. The existing gap of knowledge on metal-diatom interactions may be bridged by applying complementary spectroscopic techniques. To this aim we assess Time-Domain Terahertz Spectroscopy as a tool for sensing diatoms in their natural environment: seawater. Despite the strong absorption of terahertz radiation in water, we show that diatoms can be sensed by probing the water absorption enhancement in the terahertz range caused by the water-diatom interaction. We reveal that the addition of metal dopants affects this absorption enhancement, thus enabling the monitoring of the toxic effects of metals on diatoms by means of Terahertz Spectroscopy. In particular, we demonstrate that this technique is able to detect the detrimental effects of heavy metals earlier than conventional methods as microscopy, enzymatic assays and molecular analyses aimed at assessing the overespression of genes involved in heavy metal-stress response.
How can podiatrists contribute to reablement for older people: a qualitative study of Australian podiatrists perspectives
Authors: Mulquiny Lachlan , Oakman Jodi 
Published: May 2022

Abstract
Background: The ageing of Australia’s population is placing significant pressure on health and social aged care services due to an increasing demand for services and a relative decrease in the healthcare workforce. Reablement has been introduced by the Australian Commonwealth Government and is aimed at increasing older people's independence to age in place and decrease dependency on aged care services. To date research on reablement practice has focussed on interventions from physiotherapists, occupational therapists, and nurses, with no data available on podiatrist involvement. The aim of this research was to explore how Australian podiatrists can align their practice with the reablement model in aged care. Methods: Using a qualitative approach, semi-structured interviews were conducted with podiatrists from a diverse range of clinical settings. An interview guide was utilised which focussed on podiatrists’ current clinical practice when working with older people and to discuss how they believed the reablement model might work in the podiatry discipline. Interviews were audio-recorded, transcribed verbatim, and analysed using Braun and Clarke's approach to thematic analysis.Results: 14 podiatrists were interviewed. Using thematic analysis, three themes emerged; (i) It’s not what I was taught, “I am just a podiatrist”, (ii) Reconciling practice with competing pressures, (ii) Funding influences on podiatry practice and reablement. Podiatrists revealed that they deliver their care through a biomedical model and lack knowledge and skills in the application of the reablement model of care. Rather than finding practice examples that demonstrate involvement by podiatrists in older peoples reablement, our analysis identified a series of systemic barriers which predominantly inhibit the implementation of reablement from the podiatry discipline. Conclusions: Australian podiatrists considered their role in reablement for older people was limited. Some factors to improve podiatrists’ involvement in older peoples reablement could be addressed through training of of podiatrists in the application of holistic models of care such as reablement. However, factors such as inadequate funding arrangements involve wider health system reform and are seemingly more intractable.
Does Financial Inclusion Promote Women Empowerment with Economic Growth: A Bibliometric Approach with databases (Scopus, WOS)
Authors: KUMAR SUSHIL , ANNU ANNU , TRIPATHI RAVINDRA 
Published: May 2022

Abstract
PurposeThe study reports on the relationship among these main three keywords the financial inclusion, women empowerment, and economic growth. This study gives a pathway to a researcher in the field of this area to research with all three keywords together.Design/ Methodology/ ApproachFor the study, a Bibliometric approach has been adopted with the timespan 1997–2022 of 300 documents after extracting and cleaning the data from Scopus and Web of Science databases. ‘R’ studio and VOS viewer software have taken for the bibliometric analysis process. “The study made use of Biblioshiny, a web-based tool contained in the Bibliometrix package written in R (Ariaa and Cuccurullo, 2017)”. These are the user-friendly software for descriptive and network analysis regarding documents, authors, journals, institutions with thematic maps & three fields plot. To more exploration about the data, the paper also contains the Dimensions database’s data.Findings & ConclusionThe findings indicate about these three keywords are used frequently in this area of research but not together. Various studies found on financial inclusion and economic growth similarly women empowerment and economic development. Through this analysis, it has been found that it is also a point to research by taking all three keywords together and this study encourages the researcher to research to shed the light on issues that require additional exploration.JEL Classification: B26, E44, P43
m1A Regulator ALKBH1 and TRMT10B as Potential Prognostic Biomarkers and Related to Tumor Immune Microenvironment in Pancreatic Adenocarcinoma
Authors: Li Jiahao , Li Luohong , Huang Sike , Zhang Zhen 
Published: May 2022

Abstract
Background N1-methyladenosine (m1A) RNA methylation is crucial in the carcinogenesis and tumorous molecular pathogenesis. However, the detailed pathogenic mechanism of m1A regulators in pancreatic adenocarcinoma (PAAD) has not been comprehensively researched. Therefore, the purpose of this study was to investigate the role of m1A in PAAD prognosis and tumor microenvironment (TME).Methods The relationship between m1A regulators and the PAAD prognosis was analyzed with cBioportal database. RNA-seq transcriptome was obtained from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases for differential expression analysis. The role of m1A regulators in the PAAD TME was analyzed with TISIDB database and R software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed by The Database for Annotation, Visualization, and Integrated Discovery (DAVID).Results In PAAD, we discovered 10 m1A regulators correlated to poor prognosis and were all upregulated. While up-regulated ALKBH1 and TRMT10B were associated with a better prognosis in PAAD patients. Further research found that ALKBH1 and TRMT10B significantly upregulated the infiltrating level of immune cells, especially CD4+ T cells. Additionally, they also affected the expression of immunomodulators, including CD276, TMEM173, TNFSF9, etc. Finally, GO and KEGG enrichment analysis proved that ALKBH1 and TRMT10B influenced the PAAD development by regulating the RNA polymerase II.Conclusion Promoted expression of ALKBH1 and TRMT10B was significantly related with a better prognosis in PAAD, the possible mechanism of which might associate with the alternation of the TME. ALKBH1 and TRMT10B were potential prognosis biomarkers and possible immunotherapeutic targets for PAAD.
Role of Subjacent Plasmon Layer in Improving Photoelectrochemical Response of Metal Oxide Photoelectrodes
Authors: Srivas Anupam , Biswas Neeraj Kumar , Saxena Sakshi , Verma Anuradha , Srivast Abhishek , Dass Sahab 
Published: May 2022

Abstract
This report brings out the role of Au plasmons as the subjacent layer below TiO2 in PEC water splitting for hydrogen generation. Au was deposited on an ITO sheet using DC sputtering with 5 nm and 20 nm thickness and annealed at 300oC for 20 minutes in the N2 atmosphere. TiO2 in the form of the thin film was coated on the already deposited Au layer using a sol-gel spin coating method. Thin films were characterized using tools such as X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectroscopy, and optical absorption spectroscopy. The presence of Au as the subjacent layer in TiO2 shows enhanced absorption (visible region) due to the plasmonic properties displayed by Au nanoparticles. TiO2 with subjacent Au layer (5 nm thickness) was found to exhibit eight times higher photoresponse than the pristine sample at 1.0 V/SCE which can be ascribed to the plasmonic effect of gold nanoparticles extending absorption in the visible region of sunlight.
Extraovarian granulosa cell tumor with Call-Exner body-like microfollicular arising from ileum: a case report and review of literature
Authors: Zeng Rong , Huang Wei , Hu Peng , Guo Fang , Zhang Huifeng 
Published: May 2022

Abstract
Background. Granulosa cell tumors(GCTs) arising outside the ovary are extremely rare. Only 7 cases were reported previously. The clinical characteristics, surgery, therapy, prognosis, pathology and origin are unknown. This is the first case reported of extraovarian GCT arising from ileum. Case presentation: A 44-year-old nulliparous Chinese female presented with weight loss, anorexia and hiccups for 3 months. Surgeries revealed a 8 x7 x6cm, solid mass arising from distal ileum, separating from the adnexa and uterus of normal appearance. Postoperative pathology confirmed the primary extraovarian GCT of ileum. Only one month of disease-free after four cycles of postoperative chemotherapy was remained when metastatic tumor developed in liver. Conclusion. GCTs can arise from tissues other than ovary, may be with poor prognosis, and origin from mesenchymal tissues of embryologic genital ridge.
Exact Boolean matrix factorization based on concept lattice factors and its application in RBAC
Authors: Wu Qiang , Wang Tao , Xie Liping 
Published: May 2022

Abstract
Boolean matrix factorization methods provide representations of an object-variable data matrix by a product of two different matrices, one describing a relationship between objects and factors, and the other describing a relationship between factors and the original variables. The objective of this paper is to use indiscernibility matrix finding the matrices based on formal concept factors, and design an RBAC(Role Based Access Control) using formal concept analysis. For this purpose, we derive a formal context from the access control matrix that represents role-based access permission. We demonstrate the proposed method on an electronic medical record system of a hospital and some synthetic data, real world datasets. The analysis indicates that the proposed method can make the number of role mining relatively small and follows the RBAC constraints: static separation of duties and role hierarchy.
Small grains from Ryugu: handling and analysis pipeline for Infrared Synchrotron Microspectroscopy
Authors: Rubino Stefano , Dionnet Zélia , Aléon-Toppani Alice , Brunetto Rosario , Nakamura Tomoki , Baklouti Donia , Djouadi Zahia , Lantz Cateline , Mivumbi Obadias , Borondics Ferenc , Lefrançois Stephane , Sandt Christophe , Capitani Francesco , Héripré Eva , Troadec David , Matsumoto Megumi , Amano Kana , Morita Tomoyo , Yurimoto Hisayoshi , Noguchi Takaaki , Okazaki Ryuji , Yabuta Hikaru , Naraoka Hiroshi , Sakamoto Kanako , Tachibana Shogo , Watanabe Seiichiro , Tsuda Yuichi 
Published: May 2022

Abstract
Sample-return missions allow the study of materials collected directly from celestial bodies, unbiased by atmospheric entry effects and/or Earth alteration and contamination phenomena, using state-of-the-art techniques - but only if the collected material stays pristine. The scarcity of outer-space unaltered material recovered until now makes this material extremely precious. To maximize the scientific output of current and future sample-return missions, the scientific community needs to plan for ways of storing, handling, and measuring this precious material while preserving their pristine state for as long as the ‘invasiveness’ of measurements allows. In July 2021, as part of the Hayabusa2 (JAXA) “Stone” preliminary examination team, we received several microscopic particles from the asteroid Ryugu, with the goal of performing IR hyper-spectral imaging and IR micro-tomography studies. Here we describe the sample transfer, handling methods and analytical pipeline we implemented to study this very precious material while minimizing and surveilling their alteration history on Earth.