bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gait Alterations Associated With Mild Unilateral Knee Pain During Walking: a Machine Learning Approach Using Wearable Sensors
Authors: Bacon Kathryn L. , Felson David T. , Jafarzadeh S. Reza , Kolachalama Vijaya B. , Hausdorff Jeffrey M. , Gazit Eran , Segal Neil A. , Lewis Cora E. , Nevitt Michael C. , Kumar Deepak 
Published: July 2022

Abstract
Gait alterations in those with mild unilateral knee pain during walking may provide clues to modifiable alterations that affect progression of knee pain and osteoarthritis(OA). To examine this, we applied machine learning (ML) approaches to gait data from wearable sensors in a large observational knee OA cohort, the Multicenter Osteoarthritis (MOST) study. Participants completed a 20-meter walk test wearing sensors on their trunk and ankles. Parameters describing spatiotemporal features of gait and symmetry, variability and complexity were extracted. We used an ensemble ML technique (“super learning”) to identify gait variables in our cross-sectional data associated with the presence/absence of unilateral knee pain. We then used logistic regression to determine the association of selected gait variables with odds of mild knee pain.Of 2066 participants (mean age 63.6 [SD: 10.4] years, 56% female), 21.3% had mild unilateral pain while walking. Gait parameters selected in the ML process as influential included step regularity, sample entropy, gait speed, and amplitude dominant frequency, among others. In adjusted cross-sectional analyses, lower levels of step regularity (i.e., greater gait variability) and lower sample entropy(i.e., lower gait complexity) were associated with increased likelihood of unilateral mild pain while walking [aOR=0.80 (0.64-1.00) and aOR=0.79(0.66-0.95), respectively].
Comparison of Retinal Nerve Fiber Layer Thickness and Bruch’s Membrane Opening Minimum Rim Width Thinning Rate in Open-Angle Glaucoma
Authors: Park Donghee , Park Sung Pyo , Na Kyeong Ik 
Published: July 2022

Abstract
This study aimed to compare the rate of thinning between retinal nerve fiber layer thickness (RNFLT) and Bruch’s membrane opening minimum rim width (BMO-MRW) in open-angle glaucoma (OAG) according to glaucoma severity. We retrospectively reviewed subjects with a total of 111 eyes with OAG that had undergone optical coherence tomography more than four times during more than three years of follow-up. The subjects were divided into three groups based on the mean deviation (MD) of the baseline visual field test: high MD (MD > -2 dB), medium MD (-2 dB ≥ MD > -6 dB), and low MD (-6 dB ≥ MD > -12 dB) groups. A linear mixed model was employed to compare the rate of thinning between RNFLT and BMO-MRW among the three groups. The rate of RNFLT thinning was fastest in the inferotemporal sector in all three groups. The rate of BMO-MRW thinning was fastest in the inferotemporal sector of the high MD group and the superotemporal sector of the other two groups. Among the three groups, the rate of RNFLT thinning was not significantly different in the global sector and all sectors except the nasal sector. The rate of BMO-MRW thinning in the inferotemporal sector showed no significant difference, but that in the superotemporal sector was faster in the medium MD and low MD groups than in the high MD group. The fastest rate of RNFLT thinning was consistently observed in the inferotemporal sector, but BMO-MRW showed a change in the fastest thinning sector from inferotemporal to superotemporal, with increasing severity in early to moderate OAG. The difference in the changes in the two parameters may help understand the pathogenesis of glaucoma and predict its progression.
Riluzole partially restores RNA polymerase III complex assembly in cells expressing the leukodystrophy-causative variant POLR3B R103H
Authors: Pinard Maxime , Dastpeyman Samaneh , Poitras Christian , Bernard Geneviève , Gauthier Marie-Soleil , Coulombe Benoit 
Published: July 2022

Abstract
The mechanism of assembly of RNA polymerase III (Pol III), the 17-subunit enzyme that synthesizes tRNAs, 5S rRNA, and other small-nuclear (sn) RNAs in eukaryotes, is not clearly understood. The recent discovery of the HSP90 co-chaperone PAQosome (Particle for Arrangement of Quaternary structure) revealed a function for this machinery in the biogenesis of nuclear RNA polymerases. However, the connection between Pol III subunits and the PAQosome during the assembly process remains unexplored. Here, we report the development of a mass spectrometry-based assay that allows the characterization of Pol III assembly. This assay was used to dissect the stages of Pol III assembly, to start defining the function of the PAQosome in this process, to dissect the assembly defects driven by the leukodystrophy-causative R103H substitution in POLR3B, and to discover that riluzole, an FDA-approved drug for alleviation of ALS symptoms, partly corrects these assembly defects. Together, these results shed new light on the mechanism and regulation of human nuclear Pol III biogenesis.
The Circadian Clock is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke
Authors: Zhang Yuxing , Zhao Xin , Guo Chun , Zhang Ying , Zeng Fukang , Yin Qian , Li Zhong , Shao Le , Zhou Desheng , Liu Lijuan 
Published: July 2022

Abstract
Ischemic stroke is a major public health problem in China. Angiogenesis plays an essential role in the rehabilitation of ischemic brain injury. Recent studies demonstrated that the circadian clock is involved in the process of ischemic stroke, however, the exact mechanism of the circadian clock in regulating angiogenesis after cerebral infarction remains unclear. In the present study, we determined that environmental circadian disruption (ECD) could increase the stroke severity and impair the ability of angiogenesis in a transient middle cerebral artery occlusion (MCAO) rat model, by detecting the rat’s infarct volume, neurological test, and western blot of angiogenesis-related protein, including vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2). We further report that the core circadian clock, aryl hydrocarbon receptor nuclear translocator-like 1 (BMAL1, also known as ARNTL1), plays an irreplaceable role in angiogenesis. Overexpression of BMAL1 promotes tube forming, migration, and wound healing capacity, and upregulate the VEGF and Notch pathway protein level. While this kind of promoting effect is reversed by the Notch pathway inhibitor, DAPT, according to the results of angiogenesis capacity and VEGF pathway protein level. In conclusion, our study reveals the intervention of ECD in angiogenesis in ischemic stroke and further identifies the exact mechanism by which BMAL1 regulates angiogenesis through the VEGF-Notch1 pathway.
Identify Types of Cars from Free-View Video Images Based on Machine Learning
Authors: Xin Liu , Ajdani Mahdi 
Published: July 2022

Abstract
Urban traffic monitoring systems have played an important role in improving transportation in recent years and have led to an increase in access to such systems in most major cities around the world. Automatic analysis of traffic situation and intelligent and optimal control based on the analysis is a new and important solution in these systems. Creating an expert system that can detect and classify vehicles on roads and highways can help control traffic, monitor traffic statistics, identify offending drivers, and more. In this study, a vehicle identification system and their classification into two categories of light and heavy vehicles was performed. One of the main problems of recognizing cars on the road is that it may be taken from different angles of the car, so it is difficult to detect. To solve this problem, a dynamic pattern matching method is used to be able to in the situation. Recognize different vehicles. We have used a component-based method to classify vehicles. This method helps to improve the problem of image obstruction to some extent. The results obtained in comparison with other methods have a good result.
Exposure to family planning messages and contraceptive use among women of reproductive age in Sub-Saharan Africa: A cross-sectional program impact evaluation study
Authors: Dwomoh Duah , Amuasi Susan Ama , Amoah Emefa Modey , Gborgbortsi Winfred , Tetteh John 
Published: July 2022

Abstract
Many women of reproductive age in sub Saharan Africa are not utilizing any contraceptive method which is contributing to the high burden of maternal mortality. This study determined the prevalence, trends, and the impact of exposure to family planning messages (FPM) on contraceptive use (CU) among women of reproductive age in sub-Saharan Africa (SSA). We utilized the most recent data from demographic and health surveys across 26 SSA countries between 2013 and 2019. We assessed the prevalence and trends and quantified the impact of exposure to FPM on contraceptive use using augmented inverse probability weighting with regression adjustment. Sensitivity analysis of the impact estimate was conducted using endogenous treatment effect models, inverse probability weighting, and propensity score with nearest-neighbor matching techniques. The study involved 328,386 women of reproductive age. The overall prevalence of CU and the percentage of women of reproductive age in SSA exposed to FPM were 31.1% [95% CI: 30.6–31.5] and 38.9% [95% CI: 38.8–39.4] respectively. Exposure to FPM increased CU by 7.1 percentage points (pp) [95% CI = 6.7, 7.4] among women of reproductive age in SSA. The impact of FPM on CU was highest in Central Africa [6.7 pp; 95% CI: [5.7–7.7] and lowest in Southern Africa [2.2 pp; 95% CI: [1.3-3.0]. There was a marginal decline in the impact estimate among adolescents (estimate = 6.0 pp [95% CI = 5.0, 8.0]). Exposure to FPM has contributed to an increase in CU among women of reproductive age. Programs that are geared towards intensifying exposure to FPM through traditional media in addition to exploring avenues for appropriate use of electronic media remain critical.
ITGA9, a target of miR-146a-3p, inhibits proliferation and migration of dermal microvascular endothelial cells in psoriasis
Authors: Hou Hui , Li Jiao , Wang Juanjuan , Zhou Ling , Li Junqin , Liang Jiannan , Yin Guohua , Li Xinhua , Cheng Yueai , Zhang Kaiming 
Published: July 2022

Abstract
Background
Cell proliferation, migration, and angiogenesis are aberrant in human dermal microvascular endothelial cells (HDMECs), resulting in abnormal endothelial function and microvascular dilation.
Objective
To explore the role of Integrin subunit alpha 9 (ITGA9) in proliferation and migration of dermal microvascular endothelial cells.
Methods
HDMECs were isolated from the skin of 6 psoriatic patients and 6 healthy controls. Expression levels of ITGA9 mRNA and protein were assessed with qRT-PCR and western blot, respectively, while miqRT-PCR was used to determine expression levels of miR-146a-3p. Cell proliferation and migration were assessed in human microvascular endothelial cell line (HMEC-1), following overexpression of either ITGA9 or miR-146a-3p, or co-transfection with miR-146a-3p-mimic and pLVX - ITGA9. Cell viability was detected by Cell Counting Kit-8 assay and 5ethynyl2′deoxyuridine (EdU) cell proliferation assay. Cell apoptosis was assessed, using annexin V-FITC/PI apoptosis detection kit, while cell migration was detected by wound healing and transwell assay.
Results
Expression levels of ITGA9 were significantly decreased in psoriatic HDMECs compared to normal controls. Moreover, expression levels of miR-146a-3p were higher in psoriatic HDMECs than in normal controls. Overexpression of miR-146a-3p lowered expression levels of ITGA9, accompanied by increased proliferation and migration of HMEC-1 in vitro. In contrast, overexpression of ITGA9 inhibited proliferation and migration of HMEC-1, while increasing expression levels of cdc42, ki67, focal adhesion kinase (FAK), c-Src tyrosine kinase (Src), RAC1 and RhoA.
Conclusion
ITGA9 can repress the proliferation and migration of HMEC-1, suggesting utility of ITGA9 as a potential therapeutic intervention for psoriasis.
Machine Learning and Artificial Intelligence in Education Research: A Comprehensive Overview of 22 Years of Research indexed in the Scopus Database
Authors: Okagbue Ekene Francis , Ezeachikulo Ujunwa Perpetua , Nwigwe Esther Onyinye , Juma Amina Abedi 
Published: July 2022

Abstract
Objectives:Machine Learning (ML) and Artificial Intelligence (AI) remain crucial in education research and pedagogical development. The study provides an overview of ML and AI in education and research.Methods:In order to explore the field of ML and AI, a bibliometric approach was employed to extract and analyze data from the Scopus database. Bibliometrics analysis tools, VOS Viewer, Bibliometrics, and R packages were used to analyze the retrieved data.Results:A total of 1138 documents retrieved were written by 2800 authors, and published in 635 journals from 2000 to 2022, with average citations per document of 5.984 from 62 countries.Conclusion:The study identified an interesting gap that the previously published articles did not address: the implications of ML and AI in classroom learning activities and other pedagogical-related programmes.
Stereological Evaluation of Volume Densities of Mature Ovarian Follicular Components of Hyperprolactin Sprague-Dawley Rats Treated With Immature Coconut Water
Authors: Falana Benedict Abiola , Bakare Adeola Airat , Samson Adeleke Opeyemi , Moyosore Afodun Adam 
Published: July 2022

Abstract
Background: Hyperprolactinaemia impairs ovarian structural and functional development thereby causing anovulation and infertility. Immature coconut water (ICW) contains β-sitosterol known to be involved in the synthesis of steroid hormones in-vivo. Volume density (VD) evaluation of the ovarian follicular components has been used to measure the pattern of ovarian development and also to provide information on the physiological status of the ovaries. Thus, this study aimed to determine the effect of immature coconut water (ICW) on VD of the antrum, granulosa cell and theca cell of mature follicles in hyperprolactin rats treated with ICW.Material and Methods: A total of fifty- five (55) cyclic female Sprague-Dawley rats weighing 145-170 g were used for this study. The animals were randomly divided into four experimental study groups. Group 1 received distilled water only while group 2 rats were treated with metoclopramide hydrochloride (MCH) at 0.2 mg/100 g body weight for 28 days to induce hyperprolactinaemia and this was withdrawn for 8, 16 and 28 days to check for recovery. The animals in group 3 were post-treated with ICW for 8, 16 and 28 days and group 4, animals were treated with ICW only for 8, 16 and 28 days.Result: There were significant decreases in the VD of granulosa cell in the withdrawal groups when compared with the control. The VD of all cell types in the post-treated groups were statistically comparable with the control and the groups treated with immature coconut water only.Conclusion: Findings from this present study indicate that ICW stimulates follicular growth in hyperprolactin and control rats in the same pattern.
Association Between Hypertension and Physical Findings of COVID-19 Infected Patients; A Clinical Retrospective Study
Authors: Aslanbeigi Fatemeh , Rahimi Habiballah , Malekipour Adeleh , Pahlevani Hamed , Najafizadeh Maedeh , Ksahani Hamed Haddad 
Published: July 2022

Abstract
Background
The sudden outbreak of coronavirus disease 2019 (covid-19), which originated from severe acute respiratory syndrome coronavirus 2 (SARS-COV2) infection, has recently become an international public health emergency. The aim of this study was to assess the clinical symptoms and physical findings in both hypertensive and non-hypertensive patients infected with covid-19.
Methods
Retrospective observational study with diagnosis of corona-induced pneumonia by laboratory-confirmed test was conducted on 280 consecutive unselected patients. The demographics, laboratory, and clinical findings data were extracted from the hospital registry database.
Results
Of our two hundred and eighty patients in the study, there were 70 men (47%) and the male-female ratio was 1.17:1 and 138 (50%) were older than 60 years (mean = 67.75), and also 50 in-hospital deaths occurred (mortality rate, 17%). totally 19(6.9%) were taking tobacco, opioid, and weed. There were no significant differences in the percentages of fever, cough, sputum production, gastrointestinal symptoms, muscle ache, and headache in the both hypertensive and non-hypertensive groups. The prevalence of underlying diseases was significantly higher in older patients in comparison with younger ones (p-value = 0 < 0.05), Clinical worsening was higher in patients with hypertension relative to patients with average blood pressure (P-value = 0 < 0.05).
Conclusion
The most common comorbidities in several reports was hypertension. People with high blood pressure are also slightly more likely to experience poor prognosis and even higher rate of mortality.in the other hand, hypertension may have associated with higher risk of severe or fatal COVID-19 disease.
Iron overload causes ferroptosis but not apoptosis in MO3.13 oligodendrocytes
Authors: Li Yinghui , Wang Bingjing , Yang Jiahua , Liu Rong , Xie Junxia , Wang Jun 
Published: July 2022

Abstract
Oligodendrocytes are the most iron-rich cells in the brain. Studies have shown that oligodendrocytes are very sensitive to oxidative stress, and iron overload is more likely to cause damage to oligodendrocytes. The purpose of this experiment was to investigate the damaging effect and mechanism of ferric ammonium citrate (FAC) on MO3.13 oligodendrocytes. In FAC treatment group, the intracellular iron concentration and intracellular reactive oxygen species were increased. There were no obvious changes in nucleus and chromatin, but increased mitochondrial membrane density, decreased mitochondrial cristae and mitochondrial length were observed. Glutathione peroxidase 4 (GPX4) expression was decreased, but the ratio of Bcl-2/Bax protein levels and cleaved caspase-3 expression did not change. Moreover, the iron chelator deferoxamine and the ferroptosis inhibitor ferrostatin-1 could inhibit the upregulation of GPX4 in MO3.13 oligodendrocytes induced by iron overload. Moreover, the phosphorylation level of p53 was not changed, while the ratio of protein expressions of p-Erk1/2/Erk1/2 were markedly increased. Taken together, our data suggest that iron overload induces ferroptosis but not apoptosis in oligodendrocytes. The mechanism may be related to mitogen-activated protein kinase pathway activation rather than p53 pathway activation.
Topological Mott Insulator at Quarter Filing in the Interacting Haldane Model
Authors: Phillips Philip , Feldman Benjamin , Mai Peizhi 
Published: July 2022

Abstract
While the recent advances in topology have led to a classification scheme for electronic bands described by the standard theory of metals, a similar scheme has not emerged for strongly correlated systems such as Mott insulators in which a partially filled band carries no current. By including interactions in the topologically non-trivial Haldane model, we show that a quarter-filled state emerges with a non-zero Chern number provided the interactions are sufficiently large. We establish this result first analytically by solving exactly a model in which interactions are local in momentum space. The exact same results obtain also for the Hubbard interaction, lending credence to the claim that both interactions lie in the same universality class. From the simulations with determinantal quantum Monte Carlo, we find that the spin structure at quarter filling is ferromagnetic for the topologically non-trivial case. Possible experimental realizations in cold-atom and solid state systems are discussed.
Fluoride exposure and systemic markers of inflammation in children and adolescents in the United States: NHANES, 2013-2016
Authors: Besten Pamela Kay Den , Uyghurturk Dawud Abduweli , Wells Christine 
Published: July 2022

Abstract
Background: Ingestion of fluoride in drinking water has been shown to result in increased cellular markers of inflammation in rodent models. However, the approximately 10x greater fluoride concentrations in drinking water required by rat and mouse models to obtain blood fluoride concentrations similar those of found in humans, has made the relevance of these animal studies difficult to assess. Increased white blood cell count is a marker of inflammation in humans, and therefore we used available 2013-2016 NHANES survey data, which includes both blood cell counts and plasma fluoride concentrations, to explore the relationship between fluoride and inflammation in humans.OBJECTIVE: To assess associations between plasma fluoride levels and blood cell markers of inflammation in a US population. METHODS: Multiple linear regressions to determine the association of blood cell counts and plasma fluoride were done using publicly available NHANES survey data from the 2013-2014 and 2015-2016 cycles. Plasma fluoride concentrations were available for children aged 6 to 19, and therefore this subpopulation was used for all analyses. Covariate predictors along with plasma fluoride were age, gender, and Body Mass Index (BMI). RESULTS: Plasma fluoride was significantly positively associated with water fluoride, total white blood cell count, segmented neutrophils, monocytes, and negatively associated with red blood cell count when adjusted for age, gender and BMI. CONCLUSION: Our finding that neutrophils and monocytes are associated with higher plasma fluoride in US children and adolescents is consistent with animal data showing fluoride related effects in increasing inflammation. These findings suggest the importance of further studies to assess potential mechanisms for these fluoride related effects in tissues and organs, such as the small intestine, liver and kidney, that are involved in absorption and filtration of ingested fluoride.
Treating children with physical disabilities: a video-based educational resource using simulated participants
Authors: KImmel Alexandra , Nozetz Erin , Salisbury Mary , Okanlami Oluwaferanmi , Talwalkar Jaideep , Martin Andrés 
Published: July 2022

Abstract

Introduction: Children with physical disabilities (CWPD) have historically experienced inadequate and insensitive care across medical settings. A lack of comfort and knowledge about CWPD is prevalent amongst healthcare provider trainees. We developed a new, readily distributable educational resource about CWPD for healthcare professions students and conducted a preliminary study to determine its efficacy in addressing student attitudes towards CWPD.Methods: We collaborated with a working group of stakeholders in the disability community to develop an educational resource for healthcare students. We developed 9 short video clips (with a cumulative duration of 27 minutes) of a primary care visit using simulated participants and embedded them into a 50-minute workshop. We conducted a preliminary study of the workshop for volunteer healthcare professions students using synchronous videoconferencing. Participating students completed assessments at baseline and after the workshop. Our primary outcome measure was change in the Attitudes to Disabled Persons - Original (ATDP-O) scale.Results: Forty-nine healthcare students participated in the training session: 29 (59%) from medicine, and 21 (41%) from physician assistant or nursing programs. The materials were easy to deliver virtually. The workshop resulted in measurable changes in attitudes regarding physical disabilities, with improvement in ATDP-O scores between baseline (M=31.2, SD=8.9) and endpoint (M=34.8, SD=10.1) scores (t(49)= 3.28, p = 0.002, Cohen’s d = 0.38). Conclusions: This video-based educational resource on CWPD is readily distributable and can be delivered virtually as a workshop. In our preliminary study, the workshop improved healthcare students’ perceptions and attitudes toward CWPDs. All materials are available to view, download, or adapt by end-use instructors.