bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of the pseudoparticle system for standardized assessments of neutralizing antibodies against hepatitis C virus
Authors: Chumbe Ana , Urbanowicz Richard A. , Sliepen Kwinten , Koekkoek Sylvie M. , Molenkamp Richard , Tarr Alexander W. , Ball Jonathan K. , Schinkel Janke , van Gils Marit J. 
Published: November 2022

A better understanding of the antibody response during natural infection and the effect on disease progression and reinfection is necessary for the development of a protective hepatitis C virus (HCV) vaccine. The HCV pseudoparticle (HCVpp) system enables the study of viral entry and inhibition by antibody neutralization. A robust and comparable neutralization assay is crucial for the development and evaluation of experimental vaccines.
With the aim of optimizing the HCVpp–murine leukaemia virus (MLV) system, we tested the neutralization of HCVpp-harbouring E1E2 from 21 HCV isolates representing 6 different genotypes by several monoclonal antibodies (mAbs). HCVpps are generated by expressing functional envelope glycoproteins (E1E2) onto pseudoparticles derived from env-deleted MLV. Adjustments of E1E2, gag–pol and luciferase plasmid ratios resulted in increased yields for most HCVpps and recovery of one non-infectious HCVpp. We simplified and improved the protocol to achieve higher signal/noise ratios and minimized the amount of HCVpps and mAbs needed for the detection of neutralization. Using our optimized protocol, we demonstrated comparable results to previously reported data with both diluted and freeze–thawed HCVpps.
In conclusion, we successfully established a simplified and reproducible HCVpp neutralization protocol for studying a wide range of HCV variants. This simplified protocol provides highly consistent results and could be easily adopted by others to evaluate precious biological material. This will contribute to a better understanding of the antibody response during natural infection and help evaluate experimental HCV vaccines.
Immunohistochemical Staining Characteristics of Well Differentiated Invasive Ductal Carcinoma Using the ADH5 Cocktail (CK5/14, P63, and CK7/18): a Potential Interpretative Pitfall
Authors: Al-Refai Reham , Bendari Ahmed , Morrar Doaa , Sham Sunder , Hajiyeva Sabina 
Published: November 2022

AbstractContextIn our practice, an antibody cocktail ADH5 (CK5/14, p63, and CK7/18) helps with diagnostic challenges such as identifying microinvasion and foci of invasive carcinoma, differentiating atypical ductal hyperplasia from hyperplasia of the usual type and distinguishing basal phenotypes in triple-negative carcinomas. However, the ADH5 cocktail does have pitfalls and caveats.ObjectiveTo describe our experience with the ADH5 cocktail of antibodies in breast pathology.Data sourcesInstitutional knowledge and literature search comprise our data sources.ConclusionThe unexpected staining pattern of ADH5 in well-differentiated invasive ductal carcinomas can be challenging to interpret in these lesions with low-grade cytology. This occurrence, when using a multiplex stain, can confuse, and users should be aware of this potential pitfall.
Prevalence of Herbal Medicine use among Pregnant Women Attending Antenatal care at Jimma University Medical Center, Jimma, Southwest Ethiopia
Authors: Shuma Firafan , Bahiru Damisie , Erko Dugasa , Tekassa Tamirat 
Published: November 2022

AbstractBackgroundThe use of herbal medicine during pregnancy for different indications now a day becomes common across globally. Its use is increasing, especially in developing countries. It is estimated that 65%-80% of the population use herbal productsObjectivesThis study aimed to assess the prevalence of herbal medicine use among pregnant women attending Antenatal care at Jimma University medical center, Southwestern Ethiopia.MethodsA cross-sectional study design was employed to conduct the study. Data was collected by data collectors using data abstraction formats, then entered and analyzed using SPSS version 26.0. Frequency and percentage were computed as summary statistics.ResultsA total of 341 pregnant women attending antenatal care were enrolled in the study, of which 331 participated in the study. Out of 331 pregnant women who participated in the study, 3.93% of them practiced self-medication with herbal medicine during their current pregnancy.ConclusionsThe most frequently practiced herbal medicine among pregnant women attending antenatal care at JUMC were Ginger and Damakese with the most common clinical indication of vomiting and headache respectively. The source of information for the participant’s herbal medicine use were mostly family and friends.
Plasmodium falciparum withpfhrp2/3deletion not detected in a 2018-2021 malaria longitudinal cohort study in Kinshasa Province, Democratic Republic of the Congo
Authors: François Ruthly , Kashamuka Melchior Mwandagalirwa , Banek Kristin , Bala Joseph A. , Nkalani Marthe , Kihuma Georges , Atibu Joseph , Mahilu Georges E. , Thwai Kyaw L. , Assefa Ashenafi , Bailey Jeffrey A. , Dinglasan Rhoel R. , Juliano Jonathan J. , Tshefu Antoinette , Parr Jonathan B. 
Published: November 2022

AbstractHistidine-rich protein 2- (HRP2-) based rapid diagnostic tests (RDTs) are widely used to detectPlasmodium falciparumin sub-Saharan Africa. Reports of parasites withpfhrp2and/orpfhrp3 (pfhrp2/3)gene deletions in Africa raise concerns about the long-term viability of HRP2-based RDTs. We evaluated changes inpfhrp2/3deletion prevalence over time using a 2018-2021 longitudinal study of 1,635 enrolled individuals in Kinshasa Province, Democratic Republic of the Congo (DRC). Samples collected during biannual household visits with ≥ 100 parasites/μL by quantitative real-time PCR were genotyped using a multiplex real-time PCR assay. Among 2,726 P.falciparumPCR-positive samples collected from 993 participants during the study period, 1,267 (46.5%) were genotyped. Nopfhrp2/3deletions or mixedpfhrp2/3-intact and -deleted infections were identified in our study.Pfhrp2/3-deleted parasites were not detected in Kinshasa Province; ongoing use of HRP2-based RDTs is appropriate.
Population structure and genetic diversity ofSalmonellaTyphimurium in avian hosts
Authors: Fu Yezhi , M’ikanatha Nkuchia M. , Dudley Edward G. 
Published: November 2022

AbstractWithin-host evolution of bacterial pathogens can lead to host-associated variants of the same species or serovar. Identification and characterization of closely related variants from diverse host species are crucial to public health and host-pathogen adaptation research. However, the work remained largely underexplored at a strain level until the advent of whole-genome sequencing (WGS). Here, we performed WGS-based subtyping and analyses ofSalmonella entericaserovar Typhimurium (n= 787) from different wild birds across 18 countries over a 75-year period. We revealed seven avian host-associatedS.Typhimurium variants/lineages. These lineages emerged globally over short timescales and presented genetic features distinct fromS.Typhimurium lineages circulating among humans and domestic animals. We further showed that, in terms of virulence, host adaptation of these variants was driven by genome degradation. Our results provide a snapshot of the population structure and genetic diversity ofS.Typhimurium within avian hosts. We also demonstrate the value of WGS-based subtyping and analyses in unravelling closely related variants at the strain level.
KDM5A/B promotes HIV-1 latency and KDM5 inhibitors promote HIV-1 lytic reactivation
Authors: Li Tai-Wei , Zhou Dawei , Wu Zhenyu , Fiches Guillaume N. , Wang Xu , Park Youngmin , Jiang Wei , Ho Wen-Zhe , Badley Andrew D. , Santoso Netty G. , Qi Jun , Zhu Jian 
Published: November 2022

AbstractCombinational antiretroviral therapy (cART) effectively suppresses HIV-1 infection, replication, and pathogenesis in HIV-1 patients. However, the patient’s HIV-1 reservoir still cannot be eliminated by current cART or other therapies. One putative HIV-1 eradication strategy is “shock and kill”, which reactivates HIV-1 in latently-infected cells and induces their cytopathic effect or immune clearance to decrease the patients’ reservoir size. KDM5A and KDM5B act as the HIV-1 latency-promoting genes, decreasing the HIV-1 viral gene transcription and reactivation in infected cells. Depletion of KDM5 A/B by siRNA knockdown (KD) increases H3K4 trimethylation (H3K4me3) in HIV-1 Tat-mediated transactivation. We also found that the KDM5-specific inhibitor JQKD82 can increase H3K4me3 at the HIV-1 LTR region during HIV-1 reactivation and induce cytopathic effects. We applied the JQKD82 in combination with the non-canonical NF-κB activator AZD5582, which synergistically induced HIV-1 reactivation and cell apoptosis in HIV-1 infected cells. These results suggested that the KDM5 inhibition can be a putative HIV-1 latency-reversing strategy for the HIV-1 “shock and kill” eradication therapy.
A comparative study of neuroendocrine heterogeneity in SCLC and NBL
Authors: Cai Ling , DeBerardinis Ralph J. , Xie Yang , Minna John D. , Xiao Guanghua 
Published: November 2022

AbstractLineage plasticity has long been documented in both small cell lung cancer (SCLC) and neuroblastoma (NBL), two clinically distinct neuroendocrine (NE) cancers. In this study, we quantified the NE features of cancer as NE scores and performed a systematic comparison of SCLC and NBL. We found NBL and SCLC cell lines have highly similar molecular profiles and shared therapeutic sensitivity. In addition, NE heterogeneity was observed at both the inter- and intra-cell line levels. Surprisingly, we did not find a significant association between NE scores and overall survival in SCLC or NBL. We described many shared and unique NE score-associated features between SCLC and NBL, including dysregulation of Myc oncogenes, alterations in protein expression, metabolism, drug resistance, and selective gene dependencies. Our work establishes a reference for molecular changes and vulnerabilities associated with NE to non-NE transdifferentiation through mutual validation of SCLC and NBL samples.
The crisis of US neoliberalism and the risk of current economic stagflation
Authors: Ding Xiaoqin , Luo Zhihong 
Published: November 2022

PurposeSince the outbreak of COVID-19, tremendous changes have taken place in the US economy – the economic growth in the whole year of 2020 was negative, and though it enjoyed a significant rebound for the first half of 2021, the growth rate began to decline rapidly by the third quarter, and inflation suddenly rises rapidly, which after came the all-time highs of the “misery index” consisted of the inflation rate and unemployment rate. All signs indicate that the US economy will likely enter a “stagflation” crisis.Design/methodology/approachThis paper analyzes the institutional and social contradictions in the United States during the neoliberal era from the perspectives of domestic social structure of accumulation (SSA) and international SSA based on the SSA theory.FindingsThe current risk of stagflation in the US economy is a concentrated outbreak of the long-term accumulated contradictions in neoliberal SSA under the impact of the epidemic, which is the product of the irreconcilable contradictions inherent in the capitalist mode of production.Originality/valueBased on this analysis, the paper points out that with the deepening of the crisis, the neoliberal SSA is likely to end and a new SSA will be established gradually.
The centrality of a digital strategy for societal and business innovations
Authors: Walter Yoshija 
Published: November 2022

PurposeThe purpose of the present paper is to show that a clearly delineated digital strategy and an agile mindset is key for the successful adoption of digital innovation in society and business.Design/methodology/approachThis is a case study and critical discussion.FindingsIt was seen that both the business case (Netflix) and the societal case on the level of a country (El Salvador) lack a digital strategy, which initiated the innovation problems. However, Netflix proved to have an agile reactivity, which helped them to get back on track, which was not the case for El Salvador.Originality/valueThis discussion is relevant because the domain of “society” and the domain of “business” are often discussed as separated worlds. However, this paper intends to show that there are some logical dynamics underlying digital transformation in the case of digital innovation that apply to both of these domains.
Heat transfer characteristics of plate fin heat sink with longitudinal vortex generators
Authors: Santhanakrishnan Mani Sekaran , Tilford Timothy , Bailey Christopher 
Published: November 2022

Purpose
This study aims to provide an insight into the relationship between design parameters and thermal performance of plate fin heat sinks (PFHSs) incorporating longitudinal vortex generators (VGs) inside a PFHS channel.

Design/methodology/approach
A computational fluid dynamics model of a delta winglet pair VG mounted inside a PFHS geometry is detailed, and the model is validated by comparison with experimental data. The validated model is used to perform a virtual design of experiments study of the heat sink with bottom plate and vertical plate mounted VGs. Data from this study is used to regress a response surface enabling the influence of each of the assessed design variables on thermal performance and flow resistance to be determined.

Findings
The results of this study show that the thermal hydraulic performances of a PFHS with bottom plate mounted VG and vertical plate fin mounted VG are, respectively, 1.12 and 1.17 times higher than the baseline PFHS. Further, the performance variation of the heat sink with VG, relative to delta winglet’s arrangement (common flow up and common flow down), trailing edge gap length and Reynolds number were also evaluated and reported.

Originality/value
For the first time, performance characteristics of delta winglet VGs mounted inside the PFHS are evaluated against different design variables and a polynomial regression model is developed. The developed regression model and computed results can be used to design high performance PFHSs mounted with delta winglet VGs.
Coping with the impact of Covid-19 pandemic on primary education: teachers' struggle (case study in the Province of Yogyakarta, Indonesia)
Authors: Munastiwi Erni , Murfi Ali , Sumarni Sri , Purnama Sigit , Naimah Naimah , Istiningsih Istiningsih , Arini Annisa Dian 
Published: November 2022

PurposeThe research aimed to explore the issues in the implementation of online education practice in elementary school, to study teachers' coping strategy to the online education issues and to evaluate teachers' problem-solving skill in online learning practice during the Covid-19 pandemic.Design/methodology/approachAn exploratory research focused on identifying the obstacles in teaching practice faced by elementary school teachers as well as their coping strategy with eight convenience sampled schools.FindingsOnline education practice faced unpreparedness and competency issues. Unpreparedness was found in terms of social, technical and cultural factors, while competency issue was related to online education competency and digital competency. Teachers’ struggle to cope with the issue in online education practice was focused on the performing conventional education in the online manner, suggesting teachers' lack of competency in encouraging learning success. Teachers neglected the development of students' readiness and competencies to engage in online learning. Moreover, teachers’ struggle had the least impact on the development of their online teaching competency and digital competency that are required for carrying out online teaching. In general, teachers' problem-solving skill was below the expected level. These findings suggested that improvement of teachers' competencies is important in order to cope with the issues such as in online education practice during Covid-19 pandemic and to face future challenges in education.Originality/valueThis study evaluated the gap between actual action and expected action of elementary school teachers in coping with the issues regarding online education practice.
Use of leader–member exchange theory to promote the project governance of construction project contractors
Authors: Wang Dedong , Chen Xiaofei 
Published: November 2022

PurposeIn temporary construction project organizations, general contractors need to strengthen control over subcontractors through such measures as supervision and coordination, and resource sharing. In the management process, the good implementation of relational contracts among the general contractor and subcontractors is affected by the quality of relationship between managers and followers. From the perspective of leader–member exchange (LMX) theory, this study explores the influence of LMX, which reflects the quality of relationship between superiors and subordinates, on relational contracts.Design/methodology/approachBy combining the longitudinal influence mechanism and organizational background of relational contracts in project organizations, this study constructed a multi-level structural equation model. The hypothesis is tested based on data collected from 213 respondents.FindingsThe findings of this study show that LMX has a positive influence on relational contracts and organizational identification in construction project organizations. Organizational identification has a positive effect on relational contracts and plays a mediating role between LMX and relational contracts. Power distance plays a moderating role on the influence of LMX on organizational identification.Originality/valueThis study explores the influence of LMX on relational contracts from a new perspective, which can help establish a high-quality relation of the general contractor and subcontractors in project organizations and enriches the longitudinal study of relational contracts in project organizations.
M&A performance in emerging markets: do they behave in unison or otherwise?
Authors: Kukreja Sakshi , Maheshwari Girish C. , Singh Archana 
Published: November 2022

PurposeThe study aims to evaluate and compare the mergers and acquisitions (M&As) performance utilising a sample of deals originating from Brazil, Russia, India, China and South Africa (BRICS). In addition to nation-wise performance analysis, a further sub-sample analysis is conducted based on the target location (domestic and cross-border), development status (developed and emerging) and the acquired ownership stakes (majority and minority).Design/methodology/approachThe final sample of the study includes 7,105 deals announced between 2000 and 2019. M&A performance is proxied by the abnormal returns earned over the select event windows. Multiple parametric and non-parametric tests are employed for testing the robustness.FindingsThe results indicate significant performance differences across BRICS markets, with the highest and lowest abnormal returns reported for Chinese and Russian acquirers, respectively. The disaggregated analysis also affirms the performance differences for the select sub-samples.Research limitations/implicationsThe study highlights the need for acknowledging and expounding the differences in M&As across emerging markets. Further, the results of the study provide a possible explanation of the disagreement over the M&A performance results reported in the previous literature.Practical implicationsAcknowledging and understanding the potential performance differences based on location, ownership strategies and development status can aid executives in sharpening decision-making and also help general investors.Originality/valueThe study contributes by examining a comprehensive sample of deals across five major emerging economies, as against the majority of previous studies which have their results based on either single nation samples or have utilised only a sub-sample of domestic or foreign acquisitions.
Reflections on adolescent literacy as sociocultural practice
Authors: Agosto Denise E. 
Published: November 2022

Purpose
This paper aims to discuss the concept of “literacy” within the new literacy, new literacies and library and information science (LIS) discourses. It proposes widening the prevailing LIS conceptualization of adolescent literacy, which focuses largely on information literacy in academic settings, to a broader, information practice-based, sociocultural framing that encompasses the full range of adolescents’ everyday life contexts.

Design/methodology/approach
The author presents a literature review and personal reflection on a series of adolescent information activities to show the value of framing the LIS discourse on adolescent literacy within a broader sociocultural perspective.

Findings
Based on the discussion, the author proposes a framework for future investigations of adolescents’ literacy practices that views adolescent literacy as fundamentally social and communicative; multiformat; multicontextual; multigenerational; and culturally situated.

Originality/value
A broader sociocultural approach to the LIS information literacy discourse can lead to deeper understanding of the co-constructed and collaborative nature of adolescents’ new literacies practices. It can also enable stronger recognition of the impact of power and privilege on adolescent literacy practices. Finally, this essay shows the value of reflecting on adolescent information activities for challenging narrow views of literacy and highlights the social embeddedness of new literacies activities in adolescents’ everyday lives.
How do knowledge diversity and ego-network structures affect firms' sustainable innovation: evidence from alliance innovation networks of China's new energy industries
Authors: Zhao Yan , Qi Niannian , Li Luying , Li Zheng , Han Xiao , Xuan Ling 
Published: November 2022

PurposeFacing the global public health emergency (GPHE), the conflict of cultural differences and the imbalance of vital resources such as knowledge among different organizations are becoming more severe, which affects the enthusiasm and sustainability of firms' innovation heavily. It is an urgent problem to be solved for firms how to make use of internal knowledge and external power to help firms' sustainable innovation (FSI). Thus, the purpose of this study is to deeply analyze how firms' internal knowledge diversity (KD) and external ego-network structures [ego-network density (ED) and honest brokers (HB)] affect FSI, as well as how the ego-network structures (ED and HB) moderate the relationship between KD and FSI based on the perspective of the ego network.Design/methodology/approachBased on the data of the alliance innovation networks of China's new energy industries in 2009–2019, this study uses the social network analysis method and negative binomial regression model to explore the effect of KD and ego-network structures (ED and HB) on FSI, as well as the moderating effects of ego-network structures (ED and HB) on the relationship between KD and FSI based on the perspective of ego network.FindingsThis study finds that KD, ED and HB can boost FSI. Moreover, ED plays a negative moderating role in the relationship between KD and FSI. However, the negative moderating effect of HB on the relationship between KD and FSI is not significant.Research limitations/implicationsThis study presents fresh empirical evidence and new insights for firms on how to make full use of firms' internal KD and external ego-network structures to facilitate FSI.Originality/valueFirst, this study not only enriches the research on the consequences of KD but also expands our understanding of the knowledge-based view to some extent. Second, this study not only enriches the motivation research of the FSI based on the perspective of ego-network in the context of the GPHE but also expands the application scope of social network theory and sustainable innovation' theory in part. Third, this paper is a new attempt to apply social network theory and knowledge-based view at the same time.