bluuebunny/crossref_metadata_2025_split huggingface dataset:
Childhood maltreatment is not associated with atopic dermatitis in adults: results from a cross‐sectional population‐based cohort study
Authors: Piontek K. , Ittermann T. , Wiesmann U. , Arnold A. , Grabe H.J. , Völzke H. , Apfelbacher C. 
Published: August 2022

AbstractBackgroundChildhood maltreatment (CM) is related to poor physical and mental health outcomes in adults. Knowledge on the impact of CM on skin diseases is limited, and no study has previously addressed the association of CM with atopic dermatitis (AD) in adult age.ObjectivesTo analyse the prevalence of CM in individuals with physician‐diagnosed AD, and to examine the relationship between different types of CM with physician‐diagnosed AD in a general population sample of German adults.MethodsData from 2973 participants from the cross‐sectional population‐based Study of Health in Pomerania (SHIP) TREND‐0 were analysed (aged 20 to 83 years; 51.4% female). We administered the Childhood Trauma Questionnaire (CTQ) assessing emotional, physical and sexual abuse, and emotional and physical neglect. AD was diagnosed by dermatologists in a standardized clinical examination. We conducted logistic regression analyses adjusted for age, sex and school education to investigate the association of CM types with AD.ResultsAmong all individuals with AD, 20.6% reported to have experienced at least one type of moderate or severe CM. Emotional and physical neglect were the most frequently reported CM types. Overall, the prevalence of CM types among individuals with AD did not differ from those among individuals without AD. We found no association of CM type with AD.ConclusionsThis is the first study investigating the association of CM with AD in adults. CM was common in the present general population sample, emphasizing that CM is an important public health problem. Our findings suggest that CM is not a risk factor for AD. It might be hypothesized that AD severity is a crucial outcome, and that CM history is a factor with impact on disease severity and course rather than a risk factor for the development of AD. Longitudinal studies are required to address this question.
Transcriptional regulation of glucose transporters in human oral squamous cell carcinoma cells
Authors: Paolini Rita , Moore Caroline , Matthyssen Tamara , Cirillo Nicola , McCullough Michael , Farah Camile S. , Botha Heinrich , Yap Tami , Celentano Antonio 
Published: August 2022

AbstractThe increased glucose uptake observed in cancer cells is mediated by glucose transporters (GLUTs), a class of transmembrane proteins that facilitate the transport of glucose and other substrates across the plasma membrane. Despite the important role of glucose in the pathophysiology of oral squamous cell carcinoma (OSCC), there is very limited data regarding the expression of GLUTs in normal or malignant cells from the oral mucosa. We analysed the messenger RNA (mRNA) expression of all 14 GLUTs in two OSCC (H357/H400) and one non‐malignant oral keratinocyte (OKF6) cell line using a quantitative polymerase chain reaction. GLUT expression was evaluated at baseline and after treatment with two specific GLUT inhibitors, namely, BAY876 (GLUT1) and WZB117 (GLUT1, GLUT3 and GLUT4). Here, we show that GLUT1, GLUT3, GLUT4, GLUT5, GLUT6, GLUT8, GLUT12 and GLUT13 transcripts were measurably expressed in all cell lines while GLUT2, GLUT7, GLUT9, GLUT11 and GLUT14 were not expressed. GLUT10 was only found in H357. In the presence of BAY876 and WZB117, OSCC cells exhibited significant alterations in the transcriptional profile of GLUTs. In particular, we observed distinct proliferation‐dependent changes of mRNAs to GLUT1, GLUT3, GLUT4, GLUT5 and GLUT6 in response to selective GLUT inhibitors. In summary, we documented for the first time the expression of GLUT5, GLUT6 and GLUT12 in normal and malignant oral keratinocytes. Whilst regulation of GLUT transcripts was cell line and inhibitor specific, GLUT3 was consistently upregulated in actively proliferating OSCC cell lines, but not in OKF6, regardless of the inhibitor used, suggesting that modulation of this transporter may act as one of the primary compensation mechanisms for OSCC cells upon inhibition of glucose uptake.
Nutritional Quality of School Breakfasts: An Experience Based on Improvements of the School Food Program in Buenos Aires
Authors: Zapata María E. , Buffarini Romina , Rovirosa Alicia , Pérez Martín Joaquín , Tomé Joaquín , Carmuega Esteban 
Published: August 2022

ABSTRACTBACKGROUNDSince 2016, the School Food Program in the Buenos Aires Province, Argentina, implemented a nutritional regulatory framework (NRF) and varying administration systems (AS). Here, we examined the association between the interventions (only NRF and NRF + AS) and breakfast nutritional and food indicators between 2016 and 2019.METHODSData collected from the Survey of the School Food Program, 2016‐2019, were analyzed. The dependent variables were the energy, macro‐and micronutrients, milk/yogurt, and fruit quantities in school breakfasts. The independent variables were the school's status: (a) control group, (b) Intervention 1 (only NRF), and (c) Intervention 2 (NRF + AS). We preformed crude and adjusted linear regressions with robust variances.RESULTSWe evaluated 4,060 schools (control group: 24%; Intervention 1: 39%; Intervention 2: 37%). Only vitamin A levels increased after Intervention 1, and almost all indicators (80%) showed better values when applying Intervention 2.SCHOOL HEALTH POLICY IMPLICATIONSNutritional and food indicator improvements were associated with the implementation of the NRF and AS, emphasizing the significance of design and management of school food public policies, along with the amount of investment/territorial coverage.CONCLUSIONSOur findings highlight the importance of school food and nutrition policies with a potential impact on improving the nutritional status of children.
Transepithelial channels for leukocytes in the junctional epithelium
Authors: Oh Changseok , Kim Hyun Jung , Kim Hyun‐Man 
Published: August 2022

AbstractBackground and ObjectivesThe junctional epithelium (JE) has been recognized as a defensive organ rich in polymorphonuclear leukocytes (PMNs). However, the migration of PMNs through the JE has not been clearly documented. For mucosal defense, PMNs migrate outwards over the epithelium to defend the intestinal or respiratory tract on the epithelial surface. With this background, the present study investigated whether there is any structural evidence showing the transepithelial migration of PMNs through the JE in gingival mucosa.MethodsThree‐dimensional modeling of gingiva surrounding mouse molars at varying ages was performed by array tomography. Images of the serial sections for array tomography at the 800 nm thickness were obtained using back scattered electron (BSE) detector equipped in the field‐emission scanning electron microscopy (FESEM). Expressions of neutrophil marker or CD47 were immunohistochemically examined on the frozen sections.ResultsArray tomography using FESEM and 3‐dimensional modeling clearly showed that a system of epithelial channels developed between keratinocytes and generally ran along the long axis of the JE. Most PMNs were found inside the channels, rather than being scattered throughout the JE. The channels could be traced from the base of the JE to the bottom of the gingival sulcus, although some channels were fragmented and interrupted with short intercellular gaps.ConclusionsThese findings suggest that the JE may be an organ for transepithelial migration of PMNs to the bottom of the gingival sulcus through epithelial channels, as occurs in the epithelial lining of other organs such as the intestinal or respiratory tract.
Temporomandibular joint articular disc position and shape in skeletal Class III
Authors: Tagawa Daniella Torres , Franco Alexandre de Albuquerque , Puchnick Andrea , Wolosker Angela Maria Borri , Florez Bruna Maluza , Dominguez Gladys Cristina , Yamashita Helio Kiitiro , Cevidanes Lucia Helena Soares , Aidar Luis Antonio de Arruda , Junior Henrique Carrete 
Published: August 2022

AbstractObjectiveTo compare the position and shape of the temporomandibular joint (TMJ) articular disc among the sagittal and vertical skeletal patterns in Angle Class III, Class III subdivision malocclusion and normal occlusion. The null hypothesis was that there was no difference in disc position and shape in different (1) malocclusions and (2) skeletal patterns.MethodsThis cross‐sectional observational study evaluated 105 patients divided into 3 groups: Class III (33, 9.39 ± 1.96 years), Class III subdivision (45, 9.51 ± 1.59 years) and a normal occlusion (27, 10.24 ± 0.87 years) was included as healthy control. Severity of the maxilla‐mandibular anteroposterior discrepancy and vertical facial pattern were determined using 2D cephalometry, and the position and shape of the articular discs were evaluated in magnetic resonance images. Statistical parametric and non‐parametric tests and Kappa analysis for intra‐observer and inter‐observer assessment were used (p ≤ .05).ResultsSignificant between‐group differences were found in articular disc position. In the normal occlusion group, all the articular discs were well positioned. In Class III and Class III subdivision, the discs were displaced in 30.3% and 12.2% of the TMJs, respectively. Sagittal and vertical skeletal patterns did not affect the findings significantly. The Class III subdivision malocclusion group is probably different from the other groups, showing 97.7% of biconcave discs in both TMJs.ConclusionThe longitudinal follow‐up of this sample becomes relevant as the two groups with malocclusion in the pre‐peak phase of pubertal growth showed differences in the prevalence of displacement and form of the articular disc, with no association with their vertical facial characteristics.
3D Killer Turn Angle in Transtibial Posterior Cruciate Ligament Reconstruction Is Determined by the Graft Turning Angle both in the Sagittal and Coronal Planes
Authors: Jia Gengxin , Tang Yuchen , Liu Zhongcheng , Peng Bo , Da Lijun , Yang Jun , Liu Xiaolong , Ma Ming , Han Hua , Wu Meng , Geng Bin , Xia Yayi , Teng Yuanjun 
Published: August 2022

ObjectiveDuring the transtibial posterior cruciate ligament (PCL) reconstruction, surgeons commonly pay more attention to the graft turning angle in the sagittal plane (GASP), but the graft turning angle in the coronal plane (GACP) is always neglected. This study hypothesized that the three‐dimensional (3D) killer turn angle was determined by both the GASP and GACP, and aimed to quantitively analyze the effects of the GASP and GACP on the 3D killer turn angle.MethodsThis was an in‐vitro computer simulation study of transtibial PCL reconstruction using 3D knee models. Patients with knee injuries who were CT scanned were selected from the CT database (April 2019 to January 2021) at a local hospital for reviewing. A total of 60 3D knees were simulated based on the knees' CT data. The femoral and tibial PCL attachment were located on the 3D knee model using the Rhinoceros software. The tibial tunnels were simulated based on different GASP and GACP. The effects of the GASP and GACP on the 3D killer turn angle were quantitatively analyzed. One‐way analysis of variance was used to compare the outcomes in different groups. The regression analysis was performed to identify variables of the GASP and GACP which significantly affected 3D killer turn angle.ResultsThe 3D killer turn angle showed a significant proportional relationship not only with the GASP (r2 > 0.868, P < 0.001), but also with the GACP (r2 > 0.467, P < 0.001). Every 10° change of the GACP caused 2.8° to 4.4° change of the 3D killer turn angle, whereas every 10° change of the GASP caused 6.4° to 9.2° change of the 3D killer turn angle.ConclusionsThe 3D killer turn angle was significantly affected by both the GASP and GACP. During the transtibial PCL reconstruction, the proximal anterolateral tibial tunnel approach could increase the 3D killer turn angle more obviously compared with the most distal anteromedial tibial tunnel approach. To minimize the killer turn effect, both the GASP and GACP were required to be considered to increase.
Two novel CreERT2 transgenic mouse lines to study melanocytic cells in vivo
Authors: Stüfchen Isabel , Beckervordersandforth Ruth , Fischer Stefan , Kappelmann‐Fenzl Melanie , Bosserhoff Anja Katrin , Beyer Felix 
Published: August 2022

AbstractThe skin of adult mammals protects from radiation, physical and chemical insults. While melanocytes and melanocyte‐producing stem cells contribute to proper skin function in healthy organisms, dysfunction of these cells can lead to the generation of malignant melanoma—the deadliest type of skin cancer. Addressing cells of the melanocyte lineage in vivo represents a prerequisite for the understanding of melanoma on cellular level and the development of preventive and treatment strategies. Here, the inducible Cre‐loxP‐system has emerged as a promising tool to specifically target, monitor, and modulate cells in adult mice. Re‐analysis of existing sequencing data sets of melanocytic cells revealed that genes with a known function in neural cells, including neural stem cells (Aldh1L1 and Nestin), are also expressed in melanocytic cells. Therefore, in this study, we explored whether the promoter activity of Nestin and Aldh1L1 can serve to target cells of the melanocyte lineage using the inducible CreERT2‐loxP‐system. Using an immunohistochemical approach and different time points of analysis, we were able to map the melanocytic fate of recombined stem cells in the adult hair follicle of Nestin‐CreERT2 and Aldh1L1‐CreERT2 transgenic mice. Thus, we here present two new mouse models and propose their use to study and putatively modulate adult melanocytic cells in vivo.
Isolation and characterization of Streptomyces spp. from potato and peanut in Israel
Authors: Chalupowicz Laura , Tsror (Lahkim) Leah , Hazanovsky Marina , Erlich Orly , Reuven Michal , Dror Orit , Lebiush Sara , Manulis‐Sasson Shulamit 
Published: August 2022

AbstractPotato common scab (CS) and peanut pod wart diseases cause substantial economic losses every year in Israel. In this comprehensive study on the Streptomyces spp. population, isolates were collected during 2004–2016 from potato tubers with symptoms grown in Israel, seed tubers imported from Europe and from peanut pods with symptoms grown in Israel. A total of 142 isolates were characterized by PCR using three primer sets (txtA, tomA and nec1 genes) and by three pathogenicity tests. Seven species were identified among the isolates, S. bottropensis, S. europaeiscabiei, S. griseus, S. scabiei, S. sampsonii, S. turgidiscabies, and S. venezuelae. S. europaeiscabiei was the most dominant among isolates from imported seed tubers, while S. scabiei and S. turgidiscabies were isolated from tubers grown in Israel. Isolates originating from peanuts were significantly more virulent than those from imported seed tubers, as determined by a pathogenicity assay on radish plants. The presence of pathogenic Streptomyces was detected by quantitative PCR (qPCR) in 289 soil samples collected from 31 commercial potato fields with CS history. Eighty percent to 100% of samples collected from fields with a high disease incidence on tubers (50%) gave positive results in qPCR, whereas in samples collected from fields with low CS incidence (2%–30%) the qPCR results were very variable. Potato seed lots imported from Europe during 2008–2021 were found contaminated with CS symptoms at different levels on visual examination. The results of this study indicate that Streptomyces species, in particular S. europaeiscabiei, were introduced to Israel through seed lots imported from Europe.
Phytolith‐occluded carbon in leaves of Dendrocalamus Ronganensis influenced by drought during growing season
Authors: Li Rencheng , Chen Xiaofang , Wen Mengdan , Vachula Richard S. , Tan Shuhui , Dong Haiyan , Zhou Lintong , Gu Zhitao , Xu Mengtong 
Published: August 2022

AbstractBeing an important carbon (C) sink, phytolith‐occluded carbon (PhytOC) has been investigated in various soil–plant systems. However, the effects of environmental factors (i.e., drought) on phytoliths, including altered deposition in plant tissues, morphological variation, and amounts of carbon occluded within phytoliths, are less studied. In this study, we analyzed the monthly variations of phytolith production and PhytOC in the leaves of Dendrocalamus ronganensis grown on a karst mountain in southwestern China during a drought year. This study thus sought to understand the effects of drought on phytolith formation, morphological variations and carbon sequestration within phytoliths in plants. Our results showed that the phytolith assemblages and PhytOC between new and old leaves differed significantly and varied with plant growth stages. The average PhytOC values of old leaves and tip leaves were 3.2% and 2.2%, respectively. In particular, both PhytOC and proportions of ELONGATE, BULLIFORM FLABELLATE, and STOMA phytoliths in tip leaves significantly decreased from September to January the following year because of drought effects. This study suggests that PhytOC in plants varies between phytolith morphotypes and is significantly affected by plant growth stage and hydrologic conditions. This indicates that we can improve the efficiency of phytolith carbon sequestration in plants by improving the soil water conditions required for plant growth.
The Procreation Asymmetry Destabilized: Analogs and Acting for People's Sake
Authors: Aaron Jonas H. 
Published: August 2022

ABSTRACTIs there a pro tanto moral reason to create a life merely because it would be good for the person living it? Proponents of the procreation asymmetry claim there is not. Defending this controversial no reason claim, some have suggested that it is well in line with other phenomena in the moral realm: there is no reason to give a promise merely because one would keep it, and there is no reason to procreate merely to increase the extent of justice in the world. Allegedly, some analogs extend so far as to support a unified theory of the no reason claim and the nonidentity thesis, that is, the view that of two persons leading lives of positive wellbeing, there is a reason to create the person with higher wellbeing. I dismantle the proposed analogs and show that they fail to meet various desiderata. Moreover, I refute Johann Frick's argument that the no reason claim follows from the assumption that reasons of beneficence are reasons to act for the sake of people. By criticizing attractive defenses for the no reason claim, I weaken its plausibility.
Women adjusting their sails: The role of motility in women's livelihood strategies in a fishing village in Tamil Nadu, India
Authors: Azmi Fazeeha , Lund Ragnhild 
Published: August 2022

This article draws on research conducted in a fishing village in southern India to examine the ways in which middle‐aged women in a precarious socio‐economic position used mobility and immobility strategies to improve their livelihoods, both inside and outside of fisheries. Kaufmann et al.'s (2004) work on motility is applied to show how the mobility decisions and livelihood needs and plans of five women (occupying different socio‐economic positions) were influenced by socio‐economic structures. The study shows that the women's mobility decisions allowed them to improve their socio‐economic positions and reconfigure their gendered identities in the local village, outside the fishing sector. Motility (which encapsulates socio‐spatial mobility) empowered these women by allowing them to move out of the increasingly precarious small‐scale fisheries industry. We argue that the concept of motility provides a useful analytical framework for understanding mobility, and we use the women's narratives to exemplify this claim.
Set to fail: Affective dynamics in a resistance training program designed to reach muscle concentric failure
Authors: Bastos Vasco , Andrade Ana Jesus , Rodrigues Filipe , Monteiro Diogo , Cid Luis , Teixeira Diogo Santos 
Published: August 2022

Grounded in hedonic assumptions, evidence suggests that people tend to engage in activities they consider pleasurable and enjoyable, while trying to avoid pain and displeasure. This suggests that the dynamic between positive and negative affect can influence current behavior and the intentions to continue performing. Regarding resistance training (RT), research focusing on how to promote a better affective response is still scarce and much needed. Given existing limitations and theoretical suggestions, a RT program was developed and applied to recreational exercisers in a quasi‐experimental design aiming to (1) explore the affective response dynamic through an assessment after the last set of each exercise; and (2) analyze possible differences of preference and tolerance profiles in affective variables (core affect and enjoyment). For that purpose, 43 participants (21 male and 22 female; Mage = 34.69 ± 6.71 years; Mexperience = 8.32 ± 4.54 years; MBMI = 24.26 ± 2.64 kg/m2) accepted to participate in this study. Descriptive statistics, correlational, and group comparisons analyses were performed to provide evidence for proposed objectives. The present study showed that measures of affective valence/arousal applied immediately after a set represents a feasible and ecologically valid approach to tap core affect. Results presented evidence that recreationally trained exercisers in a common RT program would need a minimum of one measurement to assess the affective response. However, additional assessments could refine the understanding of exercise pleasurable experience. Results also suggest that exercisers with distinct profiles of preference/tolerance depicted differentiated patterns for the affective response, possibly justifying a distinct approach when promoting affective regulation.
Hospital‐level variation in rates of postpartum hemorrhage in California
Authors: Davis Rudolph , Guo Nan , Bentley Jason , Sie Lillian , Ansari Jessica , Bateman Brian , Main Elliot , Butwick Alexander J. 
Published: August 2022

AbstractBackgroundTo examine the extent of hospital‐level variation in risk‐adjusted rates of postpartum hemorrhage (PPH).Study design and MethodsWe performed a cross‐sectional study examining live births in 257 California hospitals between 2011 and 2015 using linked birth certificate and maternal discharge data. PPH was measured using International Classification of Diseases Codes version 9. Mixed‐effects logistic regression models were used to examine the presence and extent of hospital‐level variation in PPH before and after adjustment for patient‐level risk factors and select hospital characteristics (teaching status and annual delivery volume). Risk‐adjusted rates of PPH were estimated for each hospital. The extent of hospital variation was evaluated using the median odds ratio (MOR) and intraclass correlation coefficient (ICC).ResultsOur study cohort comprised 1,904,479 women who had a live birth delivery hospitalization at 247 hospitals. The median, lowest, and highest hospital‐specific rates of PPH were 3.48%, 0.54%, and 12.0%, respectively. Similar rates were observed after adjustment for patient and hospital factors (3.44%, 0.60%, and 11.48%). After adjustment, the proportion of the total variation in PPH rates attributable to the hospital was low, with a MOR of 2.02 (95% confidence interval [CI]: 1.89–2.15) and ICC of 14.3% (95% CI: 11.9%–16.3%).DiscussionWide variability exists in the rate of PPH across hospitals in California, not attributable to patient factors, hospital teaching status, and hospital annual delivery volume. Determining whether differences in hospital quality of care explain the unaccounted‐for variation in hospital‐level PPH rates should be a public health priority.