bluuebunny/crossref_metadata_2025_split huggingface dataset:
No effect of resveratrol on fatty acid oxidation or exercise capacity in patients with fatty acid oxidation disorders: A randomized clinical cross‐over trial
Authors: Storgaard Jesper H. , Løkken Nicoline , Madsen Karen L. , Voermans Nicol C. , Laforêt Pascal , Nadaj‐Pakleza Aleksandra , Tard Céline , van Hall Gerrit , Vissing John , Ørngreen Mette C. 
Published: January 2022

AbstractThe objective was to investigate whether resveratrol (RSV) can improve exercise capacity in patients with fatty acid oxidation (FAO) disorders. The study was a randomized, double‐blind, cross‐over trial. Nine patients with very long‐chain acyl‐CoA dehydrogenase (VLCAD) deficiency or carnitine palmitoyl transferase (CPT) II deficiency were randomized to receive either 8 weeks of 1000 mg day−1 RSV or placebo (P) followed by a 4‐weeks wash‐out period and subsequently 8 weeks of the opposite treatment. Primary outcome measures were heart rate and FAO as measured via stable isotope technique during constant workload exercise. Secondary outcome measures included fat and glucose metabolism; perceived exertion; as well as subjective measures of energy expenditure, fatigue, and daily function. Eight participants completed the trial. Heart rate did not differ at the end of exercise after treatment with RSV vs placebo (P = .063). Rate of oxidation of palmitate at end of exercise was not different with 1.5 ± 0.8 (RSV) vs 1.3 ± 0.6 (P) μmol kg−1 min−1 (P = .109). Secondary outcomes did not change except for increased plasma glycerol and decreased plasma glucose levels at the end of exercise after treatment with RSV vs placebo. A daily dose of 1000 mg resveratrol does not improve exercise capacity or FAO during exercise in patients with CPTII or VLCAD deficiencies.
Effects of dietary supplementation with multispecies probiotics on intestinal epithelial development and growth performance of neonatal calves challenged with Escherichia coli K99
Authors: Wu Yan‐yan , Nie Cun‐xi , Xu Chunsheng , Luo Rui‐qing , Chen Hong‐li , Niu Jun‐li , Bai Xue , Zhang Wenju 
Published: January 2022

AbstractBACKGROUNDProbiotics exhibit antibiotic properties and are capable of treating certain bacterial infections, including diarrhea. Therefore, the aim of this study is to investigate the effects of dietary supplementation with multispecies probiotic (MSP) on diarrhea, average daily gain (ADG) and intestinal development of neonatal calves challenged with Escherichia coli K99.RESULTSThirty‐six neonatal Holstein calves were randomly assigned to three treatment groups. After E. coli K99 challenge, calves in the control (C) and MSP treatment groups had significantly higher ADG and feed efficiency, and significantly lower fecal scores than those of calves in the diarrhea (D) group. The mean time of diarrhea resolution was 4.5 and 3.1 days for calves in the D and MSP treatment groups, respectively. Furthermore, the structures of the various segments (duodenum, jejunum and ileum) of the small intestine of the calves, activities of several small intestinal enzymes, and expression of several energy metabolism‐related genes in the small intestine segments were significantly affected by MSP treatments.CONCLUSIONDietary supplementation of MSP had a positive effect in treating calf diarrhea; it improved ADG and feed efficiency and promoted development of the small intestine. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Association of genomic profiles and survival in early onset and screening‐age colorectal cancer patients with liver metastases resected over 15 years
Authors: Narayan Raja R. , Aveson Victoria G. , Chou Joanne F. , Walch Henry S. , Sanchez‐Vega Francisco , Santos Fernandes Gustavo Dos , Balachandran Vinod P. , D'Angelica Michael I. , Drebin Jeffrey A. , Jarnagin William R. , Wei Alice C. , Cercek Andrea , Gönen Mithat , Schultz Nikolaus , Kingham T. Peter 
Published: January 2022

AbstractBackgroundThis study explores whether genomic profiles of colorectal liver metastasis (CRLM) patients with early onset (EO, < 50 years old) and screening age (SA) primary diagnosis are associated with overall survival (OS).MethodsAll patients undergoing hepatectomy between 2002 and 2017 were identified and tumor specimens with next‐generation sequencing data were cataloged. Gene and signaling‐level alterations were checked for association with OS from primary diagnosis accommodating for left‐truncated survival.ResultsOf 1822 patients, 333 were sequenced–127 (38%) EO‐CRLM and 206 (62%) SA‐CRLM patients. More aggressive features presented in EO‐CRLM patients–synchronous metastatic presentation (83% vs. 75%,p < 0.001) and primary node‐positive disease (71% vs. 61%,p < 0.001). The median OS from primary diagnosis was 11.8 years (95% confidence interval = 7.94‐NA). Five‐year OS did not differ by age (p = 0.702). On multivariable analysis, alteredAPC(EO‐CRLM: [hazard ratio [HR] = 0.37,p = 0.018] vs. SA‐CRLM:[HR = 0.61,p = 0.260]),BRAF(EO‐CRLM:[HR = 4.38,p = 0.007] vs. SA‐CRLM:[HR = 4.78,p = 0.032]), and RAS‐TP53(EO‐CRLM:[HR = 2.82,p = 0.011] vs. SA‐CRLM:[HR = 2.35,p = 0.003]) associated with OS.ConclusionsDespite bearing more aggressive features, EO‐CRLM patients had similar genomic profiles and survival as SA‐CRLM patients. Better performance status in younger patients leading to increased treatment tolerance may partly explain this. As screening and treatment strategies from older patients are applied to younger patients, genomic predictors of biology identified historically in older cohorts could apply to EO patients.
Transcervical inflatable mediastinoscopic esophagectomy versus thoracoscopic esophagectomy for local early‐ and intermediate‐stage esophageal squamous cell carcinoma: A propensity score‐matched analysis
Authors: Chen Zihao , Huang Kenan , Wei Rongqiang , Liu Chengdong , Fang Yunhao , Wu Bin , Xu Zhifei , Ding Xinyu , Tang Hua 
Published: January 2022

AbstractBackground and objectiveTranscervical inflatable mediastinoscopic esophagectomy (TIME) is a novel method of minimally invasive esophagectomy (MIE) for esophageal cancer. However, whether TIME is effective and feasible as conventional MIE remains unclear. This study aimed to evaluate the efficacy of TIME by comparing it with thoracoscopic esophagectomy (TE).MethodsSurgical outcomes and relapse‐free survival (RFS) rates of patients with local early‐ or intermediate‐stage thoracic esophageal squamous cell carcinoma that underwent TIME or TE from January 2017 to December 2019 were analyzed in this retrospective study. Propensity score matching was used to control the confounding factors.ResultsThe mean operation time in TIME was shorter than that in TE (p < 0.05). Patients in the TIME group achieved postoperative ambulation earlier than those in the TE group (p < 0.05). The rate of pulmonary complications was lower in TIME than in TE (p < 0.05). The number of lymph nodes harvested during surgery and the RFS rates of two groups did not have significant differences.ConclusionTIME may be a feasible and safe method to treat local early‐ and intermediate‐stage thoracic esophageal squamous cell carcinoma effectively and it could be a supplementary surgical method of TE for patients with poor pulmonary function or cannot undergo TE.
Clinical endpoints of needle‐free jet injector treatment: An in depth understanding of immediate skin responses
Authors: Bik Liora , van Doorn Martijn B. A. , Boeijink Neill , Wennekers Medelyn , Meesters Arne A. , Bloemen Peter , Haedersdal Merete , Wolkerstorfer Albert 
Published: January 2022

AbstractObjectivesNeedle‐free jet injectors have been used in dermatological practice for many years. However, predefined clinical endpoints that guide physicians to choose optimal device settings have not been clearly defined. Here, we evaluate immediate skin responses as clinical endpoints for needle‐free jet injector treatments.MethodsWe injected methylene blue in ex vivo human skin using an electronically‐controllable pneumatic injector (EPI; 3–6 bar, 50–130 µl; n = 63), and a spring‐loaded jet injector (SLI) with fixed settings (100 µl; n = 9). We measured the immediate skin papule (3D‐camera), residual surface fluid (pipette), dermal dye distribution by estimating depth and width, and subcutaneous dye deposition.ResultsEPI with 4 bar and 100 µl resulted in the largest skin papule of 48.7 mm3 (35.4–62.6 mm3) and widest dermal distribution of 8.0 mm (5.5–9.0 mm) compared to EPI with 6 bar and 100 µl (p < 0.001, p = 0.018, respectively). The skin papule volume showed a significant moderate to high positive correlation with the width and depth of dye distribution in the dermis (rs = 0.63, rs = 0.58, respectively; p < 0.001 for both correlations). SLI showed high variability for all outcome measures. Finally, a trend was observed that a small skin papule (≤7 mm) and little residual surface fluid (≤10% of injection volume) were warning signs for subcutaneous deposition.ConclusionsThe immediate skin papule and residual surface fluid correspond with dermal drug deposition and are relevant clinical endpoints for needle‐free jet injector treatments in dermatological practice.
AAPM Task Group Report 290: Respiratory motion management for particle therapy
Authors: Li Heng , Dong Lei , Bert Christoph , Chang Joe , Flampouri Stella , Jee Kyung‐Wook , Lin Liyong , Moyers Michael , Mori Shinichiro , Rottmann Joerg , Tryggestad Erik , Vedam Sastry 
Published: January 2022

AbstractDose uncertainty induced by respiratory motion remains a major concern for treating thoracic and abdominal lesions using particle beams. This Task Group report reviews the impact of tumor motion and dosimetric considerations in particle radiotherapy, current motion‐management techniques, and limitations for different particle‐beam delivery modes (i.e., passive scattering, uniform scanning, and pencil‐beam scanning). Furthermore, the report provides guidance and risk analysis for quality assurance of the motion‐management procedures to ensure consistency and accuracy, and discusses future development and emerging motion‐management strategies. This report supplements previously published AAPM report TG76, and considers aspects of motion management that are crucial to the accurate and safe delivery of particle‐beam therapy. To that end, this report produces general recommendations for commissioning and facility‐specific dosimetric characterization, motion assessment, treatment planning, active and passive motion‐management techniques, image guidance and related decision‐making, monitoring throughout therapy, and recommendations for vendors. Key among these recommendations are that: (1) facilities should perform thorough planning studies (using retrospective data) and develop standard operating procedures that address all aspects of therapy for any treatment site involving respiratory motion; (2) a risk‐based methodology should be adopted for quality management and ongoing process improvement.
Retracted: Monitoring Hatchery Broodstock Composition and Genetic Variation of Spring/Summer Chinook Salmon in the Columbia River Basin with Multigeneration Pedigrees
Authors: Horn Rebekah L. , Hess Maureen , Harmon Stephanie , Hess Jon , Delomas Thomas A. , Campbell Matthew R. , Narum Shawn 
Published: January 2022

Abstract
Hatchery production of Chinook Salmon Oncorhynchus tshawytscha in the Columbia River basin comprises most of the anadromous salmonid production in this region. Hatchery facilities and programs serve to mitigate for impacts to salmonids due to the construction and operation of hydropower dams and habitat impacts from development in addition to the conservation and restoration of natural populations. A genetic method referred to as parentage-based tagging (PBT) enables highly reliable detection of hatchery-origin fish and inference of multigeneration pedigrees. This study compiles 11 years of PBT data from nearly 125,000 interior stream-type Chinook Salmon from 24 spawning hatcheries located in tributaries of the mid- and upper Columbia River as well as the Salmon, Clearwater, and Grande Ronde River subbasins. Multigenerational pedigrees allowed investigation of the proportion of natural- and hatchery-origin broodstock (pNOB and pHOB, respectively) for each hatchery and enumeration of the scale of production between segregated and integrated programs. We then compared how pHOB and the scale of production influenced the number of stray fish observed, genetic diversity, relatedness, and age-class compositions within broodstocks. Over 91.0% of hatchery broodstock could be assigned back to their parents, and there was an overall rate of less than 1.0% of broodstock that were unintentionally incorporated into nonnatal hatchery programs. We evaluated 14 segregated programs with 0.0–10.0% pNOB, 7 intermediate programs (10.1–50.0% pNOB), and 3 integrated programs (&gt;50.0% pNOB). There was no correlation between the scale of production or pNOB with the level of genetic diversity, but as production size increased, so did the effective number of breeders. This study demonstrates the utility of PBT as a monitoring tool for hatchery broodstocks, and results suggest that segregated and integrated programs have tradeoffs that generally align with their intended broodstock management purpose of providing fish for harvest and/or fish for supplementation or reintroduction.
Genetic context of oncogenic drivers dictates vascular sarcoma development in aP2‐Cre mice
Authors: Hanna Jason A , Langdon Casey G , Garcia Matthew R , Benton Annaleigh , Lanman Nadia A , Finkelstein David , Rehg Jerold E , Hatley Mark E 
Published: January 2022

AbstractAngiosarcomas are aggressive vascular sarcomas that arise from endothelial cells and have an extremely poor prognosis. Because of the rarity of angiosarcomas, knowledge of molecular drivers and optimized treatment strategies is lacking, highlighting the need for in vivo models to study the disease. Previously, we generated genetically engineered mouse models of angiosarcoma driven by aP2‐Cre‐mediated biallelic loss of Dicer1 or conditional activation of KrasG12D with Cdkn2a loss that histologically and genetically resemble human tumors. In the present study, we found that DICER1 functions as a potent tumor suppressor and its deletion, in combination with either KRASG12D expression or Cdkn2a loss, is associated with angiosarcoma development. Independent of the genetic driver, the mTOR pathway was activated in all murine angiosarcoma models. Direct activation of the mTOR pathway by conditional deletion of Tsc1 with aP2‐Cre resulted in tumors that resemble intermediate grade human kaposiform hemangioendotheliomas, indicating that mTOR activation was not sufficient to drive the malignant angiosarcoma phenotype. Genetic dissection of the spectrum of vascular tumors identified genes specifically regulated in the aggressive murine angiosarcomas that are also enriched in human angiosarcoma. The genetic dissection driving the transition across the malignant spectrum of endothelial sarcomas provides an opportunity to identify key determinants of the malignant phenotype, novel therapies for angiosarcoma, and novel in vivo models to further explore angiosarcoma pathogenesis. © 2022 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.
The multitargeted receptor tyrosine kinase inhibitor sunitinib induces resistance of HER2 positive breast cancer cells to trastuzumab-mediated ADCC
Authors: Guti Eliza , Regdon Zsolt , Sturniolo Isotta , Kiss Alexandra , Kovács Katalin , Demény Máté , Szöőr Árpád , Vereb György , Szöllősi János , Hegedűs Csaba , Polgár Zsuzsanna , Virág László 
Published: January 2022

AbstractDespite recent advances in the development of novel personalized therapies, breast cancer continues to challenge physicians with resistance to various advanced therapies. The anticancer action of the anti-HER2 antibody, trastuzumab, involves antibody-dependent cell-mediated cytotoxicity (ADCC) by natural killer (NK) cells. Here, we report a repurposing screen of 774 clinically used compounds on NK-cell + trastuzumab-induced killing of JIMT-1 breast cancer cells. Using a calcein-based high-content screening (HCS) assay for the image-based quantitation of ADCC that we have developed and optimized for this purpose, we have found that the multitargeted tyrosine kinase inhibitor sunitinib inhibits ADCC in this model. The cytoprotective effect of sunitinib was also confirmed with two other assays (lactate dehydrogenase release, and electric cell substrate impedance sensing, ECIS). The drug suppressed NK cell activation as indicated by reduced granzyme B deposition on to the target cells and inhibition of interferon-γ production by the NK cells. Moreover, sunitinib induced downregulation of HER2 on the target cells' surface, changed the morphology and increased adherence of the target cells. Moreover, sunitinib also triggered the autophagy pathway (speckled LC3b) as an additional potential underlying mechanism of the cytoprotective effect of the drug. Sunitinib-induced ADCC resistance has been confirmed in a 3D tumor model revealing the prevention of apoptotic cell death (Annexin V staining) in JIMT-1 spheroids co-incubated with NK cells and trastuzumab. In summary, our HCS assay may be suitable for the facile identification of ADCC boosting compounds. Our data urge caution concerning potential combinations of ADCC-based immunotherapies and sunitinib.
Curcumin sensitizes response to cytarabine in acute myeloid leukemia by regulating intestinal microbiota
Authors: Liu Junmin , Luo Wei , Chen Qiuru , Chen Xing , Zhou Gang , Sun Hongbo 
Published: January 2022

Abstract
Purpose
To address whether Curcumin has synergistic effect with cytarabine (Ara-C) in treating acute myeloid leukemia (AML).

Methods
A xenograft AML mouse model was established by injecting HL-60 cells into tail vein of mice to assess the function of Curcumin. Mononuclear cells (MNCs) isolated from AML mice and AML cell lines were used to examine the effect of Curcumin. Metagenomics and metabolomics were used to evaluate the alteration of intestinal microbiota and the change of metabolites in MNCs.

Results
Curcumin treatment sensitized response to Ara-C in MNCs of AML mice, but had no direct effect on AML cell lines. Metagenomics revealed an alteration of intestinal microbiota with Curcumin treatment, which contributes to sensitized response to Ara-C. Curcumin treatment led to enhanced intestinal intact to sensitize response to Ara-C in AML mice, through reducing mucus degrading bacteria. Metabolomics demonstrated that Curcumin treatment led to decreased cholesterol in MNCs of AML mice. Further study proved that Curcumin treatment resulted in inhibition of SQLE, a key enzyme of cholesterol biosynthesis, to increase sensitivity to Ara-C.

Conclusion
Curcumin sensitizes response to Ara-C through regulating microbiota, highlighting the importance of intestinal intact strengthening in chemoresistant therapy. Moreover, aiming at cholesterol synthesis is promising in AML treatment.
Single photon sources for quantum radiometry: a brief review about the current state-of-the-art
Authors: Kück Stefan , López Marco , Hofer Helmuth , Georgieva Hristina , Christinck Justus , Rodiek Beatrice , Porrovecchio Geiland , Šmid Marek , Götzinger Stephan , Becher Christoph , Fuchs Philipp , Lombardi Pietro , Toninelli Costanza , Trapuzzano Marco , Colautti Maja , Margheri Giancarlo , Degiovanni Ivo Pietro , Traina Paolo , Rodt Sven , Reitzenstein Stephan 
Published: January 2022

AbstractSingle-photon sources have a variety of applications. One of these is quantum radiometry, which is reported on in this paper in the form of an overview, specifically of the current state of the art in the application of deterministic single photon sources to the calibration of single photon detectors. To optimize single-photon sources for this purpose, extensive research is currently carried out at the European National Metrology Institutes (NMIs), in collaboration with partners from universities. Single-photon sources of different types are currently under investigation, including sources based on defect centres in (nano-)diamonds, on molecules and on semiconductor quantum dots. We will present, summarise, and compare the current results obtained at European NMIs for single-photon sources in terms of photon flux, single-photon purity, and spectral power distribution as well as the results of single-photon detector calibrations carried out with this type of light sources.
Association of sweetened carbonated beverage consumption during pregnancy and ADHD symptoms in the offspring: a study from the Norwegian Mother, Father and Child Cohort Study (MoBa)
Authors: Kvalvik Liv Grimstvedt , Klungsøyr Kari , Igland Jannicke , Caspersen Ida Henriette , Brantsæter Anne Lise , Solberg Berit Skretting , Hartman Catharina , Schweren Lizanne Johanna Stephanie , Larsson Henrik , Li Lin , Forthun Ingeborg , Johansson Stefan , Arias Vasquez Alejandro , Haavik Jan 
Published: January 2022

Abstract
Purpose
Intrauterine exposures influence offspring health and development. Here we investigated maternal intake of sweetened carbonated beverages (SCB) during pregnancy and its association with ADHD symptoms in the offspring.

Methods
This study was based on the Norwegian Mother, Father and Child Cohort Study (MoBa) and the Medical Birth Registry of Norway. Maternal diet mid-pregnancy was assessed using a food frequency questionnaire (FFQ). All mothers who responded to the FFQ and a questionnaire when their child was 8 years of age were included (n = 39,870). The exposure was defined as maternal intake (daily servings) of SCB, using no daily intake as reference. Outcome was offspring ADHD symptoms, evaluated as a continuous standardized ADHD score and as a binary outcome of six or more ADHD symptoms vs. five symptoms or less. Associations were analysed using log-binomial regression and linear mixed regression models with adjustment for covariates.

Results
The adjusted regression coefficients for the standardized ADHD offspring symptom score were 0.31 [95% confidence intervals (0.001, 0.62)] and 0.46 (0.15, 0.77) for maternal daily intake of ≥ 1 glasses of SCB, when the models included adjustments for total energy intake or energy intake from other sources than SCBs and sweet drinks, respectively. The corresponding adjusted relative risks were 1.16 (1.004, 1.34) and 1.21. (1.05, 1.39) for drinking ≥ 1 glasses daily.

Conclusion
In a large pregnancy cohort with offspring followed until 8 years of age, we found an association between maternal daily intake of SCB and offspring ADHD symptoms. These results suggest a weak positive relationship between prenatal exposure to SCB and offspring ADHD.