bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of adjuvant application of corticosteroid and ozone after ultrasound-guided puncture and lavage for the treatment of rotator cuff calcifific tendinitis: study protocol for a non-inferiority randomized controlled trial
Authors: Dong Jing , Zhang Lan , Jia Haibin , Zhu Yuanjiang , Xiang Rui , Li Peiyu 
Published: December 2022

Abstract
Background: Steroid injection after percutaneous irrigation of calcific deposits is a common method for treatment of rotator cuff calcifific tendinitis(RCCT). However, steroid may prevent calcification resorption and cause potential irreversible damage to tendons. Recent studies have confirmed the positive effects of ozone injection in shoulder tendinopathies,but the application in RCCT has not been reported.Thus, our study aims to evaluate the non-inferiority of ozone versus steroid injection.
Methods: This is a prospective, randomized, parallel control and non-inferiority trial. A total of 100 patients with unilateral symptomatic RCCT will be enrolled and randomised in a 1:1 ratio to two groups: ultrasound-guided injection with ozone or corticosteroid.The primary outcome is the numeric rating scale for pain(NRS) at 1 week and 3 months following the procedure. Secondary outcomes include multi- dimensional evaluation of shoulder disability and quality of life improvement,degree of calcification absorption after treatment and the number of multiple treatments.
Discussion: The results of this study will provide short-term and long-term evidence for ozone treatment of RCCT in relieving pain or improving shoulder function.
Trial registration: Chinese Clinical Trial Registry ChiCTR2200063469.Registered on 7 September 2022.
Effects of remote ischemic conditioning on conditioned pain modulation and cardiac autonomic modulation in women with knee osteoarthritis: placebo-controlled randomized clinical trial protocol
Authors: Biral Taíse Mendes , Cavina Allysiê Priscilla de Souza , Junior Eduardo Pizzo , Filho Carlos Alberto Toledo Teixeira , Vanderlei Franciele Marques 
Published: December 2022

Abstract
Background

It is estimated that over 240 million people worldwide have osteoarthritis, which is a major contributor to chronic pain and central changes in pain processing, including endogenous pain modulation. The autonomic nervous system plays a crucial role in the pain regulatory process. One of the main mechanisms of remote ischemic conditioning is neuronal signaling from the preconditioned extremity to the heart. This study aims to analyze the acute effect of remote ischemic conditioning on local pain, conditioned pain modulation and cardiac autonomic control in women with knee osteoarthritis and to see if there is a correlation between them.

Methods

Randomized, placebo-controlled, double-blind clinical trial. Participants will be divided into: i) remote ischemic conditioning in which an adapted cuff will be positioned in the proximal region of the thigh of the lower limb with the greatest complaint of knee pain and a protocol consisting of four cycles of total ischemia, followed immediately by four cycles of five minutes of vascular reperfusion, totaling 40 minutes, will be performed; ii) placebo remote ischemic conditioning that differs from the previous one in relation to ischemia of 10 mmHg. The main outcomes evaluated will be the conditioned pain modulation in which the test stimulus will be performed through the pressure pain threshold and the conditioning stimulus through the cold pressor test and cardiac autonomic modulation through heart rate variability. Comparisons will be performed using generalized linear mixed models fitted to the data. For correlation, the Pearson or Spearman test will be used depending on the normality of the data. All analyzes will assume a significance level of p < 0.05.

Discussion

the results of this study are believed to show a new perspective for the interaction between pain processing and cardiovascular systems; in addition to providing the therapist and patient with better conditions to ensure greater cardiovascular safety in the use of the intervention and trigger knowledge of an acute response for future chronic intervention strategies that aim to be used in the clinical environment as another strategy that can help, within the multimodal approach, for the management of knee osteoarthritis.
Trial registration number: ClinicalTrials.gov, (NCT05059652). Registered on 27 September 2021; Last Update 19 April 2022.
Optimization of absenteeism indicators for a face recognition based syndromic surveillance system: a longitudinal study, China, September 2021 to June 2022
Authors: Wang Wei Ye , Huang Xiao Liu , Yang Zhen 
Published: December 2022

Abstract
Background
Although smart attendance can only collect all-cause absenteeism currently, whichis conductive to the modernization of school-oriented syndromic surveillance system (SSS).It is of great significance to optimize indicators of all-cause absenteeism based on smart attendance.
Aim
To choose an appropriate time standard for absenteeism, and explore more effective indicators for the face-recognition-based SSS (FRSSS).
Methods
Two primary schools in Hangzhou, China participated in the pilot study. Grade 1-2 (DARL), 3-6 (DARH), and school-wide (DARX) all-cause-absenteeism reported by FRSSS, and all-cause (DARY) and sickness absenteeism (DARZ) reported by school physicians, were daily collected from September 1, 2021, to June 24, 2022, and these five indicators' effectiveness of epidemic detection were compared by correlations, time series, and control charts.
Results
The time standard of absenteeism was "≥ 24 hours" for DARY and DARZ, while "≥ one hour" for DARX, DARL and DARH. DARY and DARZ only were 32.6% and 25.2% of DARX. The correlation coefficient between DARY and DARZ was 0.843 (P<0.001) in school A and 0.933 (P<0.001) in school B. In school A, Yoden indexes of DARL, DARH, DARX, DARY and DARZ were 83.0%, 85.0%, 80.6%, 78.2% and 80.4%, respectively. In school B, Yoden indexes of these five indicators were 89.3%, 91.0%, 83.9%, 76.8% and 81.0%, respectively.
Conclusions
The effectiveness of outbreak detection for the smart attendance based indicators could be raised to a considerable level by setting reasonable time standard and adopting multi-level indicators. It is feasible and effective to popularize smart attendance in school-oriented SSSs.
Pre-Hospital Delays among Patients with Acute Coronary Syndrome in the Russian Federation: a multicentre prospective observational cohort study (the AMIR Study)
Authors: Bates Katie , Schirmer Henrik , Kontsevaya Anna , Bobrova Natalia , Leon David A. , McKee Martin 
Published: December 2022

Abstract
Objective To describe total pre-hospital delays (symptom onset to admission), patient delay (symptom onset to calling for help), and transport delay (calling for help to admission) experienced by patients with acute coronary syndrome (ACS) in Russia and to identify factors associated with longer delays.
Methods A prospective observational cohort study of ACS patients with myocardial infarction recruited in both PCI and non-PCI hospitals across 13 regions and multiple levels of the health system in Russia.
Results Median total pre-hospital delays in our study was 5.1 hours, median patient delay was 1.5 hours and median transport delay was 2.1 hours. Patient delay did not differ by age and sex, although having a STEMI and certain symptom presentations, including severe pain, reduced delays. Transport delays were markedly reduced in those transported by Emergency Medical Services (EMS) and taken directly to the hospital of definitive treatment (rather than one closer but unable to offer Percutaneous coronary intervention (PCI)).
Whilst transport delays were reduced in those using EMS, just half of patients using EMS had a transport delay of less than 2 hours (first medical contact to admission). Among all patients taken directly to hospital by EMS, 70% were STEMI patients. Of these STEMI patients, 78% had a transport delay of 2 hours or more. Among these patients, only 16.0% received thrombolysis in the ambulance. As expected, regional differences were apparent with all types of delay, with greatest variation found in transport delays.
Conclusions Delays are currently longer than the ESC guidelines for STEMI patients and other severe cases., Reducing patient delay will reduce overall delays. Transport delays are inevitable in many regions of Russia but better triage of patients, increased use of EMS, and early thrombolysis in EMS, particularly for STEMI patients facing excessive transport delays, will reduce delays and buy time before PCI.
The relationship between metabolic associated fatty liver disease and thyroid nodule: A Cross-sectional Study in China
Authors: Xiao Jun , Tian Shen , Wu Juan , Xu Zhou , Li Hao , Li Shu , Liang Xin-yu , Chen Yu-ling , Adhikari Vishnu Prasad , Song Jing-yu , Ma Chen-yu , She Rui-ling , Li Zhao-xing , Ran Liang , Kong Ling-quan 
Published: December 2022

Abstract
Background: The relationship between metabolic associated fatty liver disease (MAFLD) and thyroid nodule (TN) is unknown, despite the obese individuals showed a greater risk of nonalcoholic fatty liver disease（NAFLD）and thyroid cancer compared to the normal BMI individuals. . This is a cross-sectional study examining the association between TN and MAFLD in healthy Chinese population undergoing simultaneous abdominal and thyroid ultrasonography.
Methods： A total of 78077 healthy individuals with complete biochemical, thyroid and liver ultrasonography data from the Quality Control Center of Health Examination in Chongqing, China were included in the study. TNs were classified into malignancy risk levels of 5 different categories in accordance with Thyroid Imaging Reporting and Data System(TI-RADS). We investigated the association between MAFLD and TNs by binomial logistic regression analyses，and multinomial logistic regression analyses were performed to test the independence of associations between TNs or different TI-RADS categories of TNs and MAFLD.
Findings: The prevalence of TN, MAFLD and combined TN with MAFLD were 30.4% (female: 36.7%, male: 26%), 26.9% (female: 13.4%, male: 36.4%) and 8.8% (female: 10.1%, male: 6.8%), respectively. The males with MAFLD had a higher risk of TN after adjustment for age, gender, blood pressure and some hepatic and renal function indicators, males with MAFLD had a higher risk of TN (OR=1.108 ,95%CI: 1.054-1.165，P<0.001)and TN of TI-RADS≥4A (OR=1.231 ,95%CI：1.158-1.308，P<0.001) compared to males without MAFLD; Furthermore, with stratification on the basis of BMI, only non-obese men with MAFLD had a higher risk of TN (OR=1.100 ,95%CI：1.016-1.191，P=0.019) and TN of TI-RADS≥4A (OR=1.213, 95%CI：1.099-1.340，P<0.001). However, no statistical risk of TN or TN of TI-RADS≥4A was observed in females with MAFLD.
Conclusion: The prevalence of MAFLD and TN is high in Chinese healthy population with difference between males and females; The association between TN or TN of TI-RADS≥4A and MAFLD is only observed in non-obese males.
Clinical and economic impact of pneumonia and acute respiratory infection related with influenza vaccination in diabetic patients or patients with chronic obstructive pulmonary disease in Colombia, 2013-2016
Authors: Dario Londoño , Fernando Valderrama José , Ludy Parada , Marco Acosta , Alejandra Taborda 
Published: December 2022

Abstract
Background
Influenza is a public health problem and has important outcomes in at-risk populations such as children, pregnant women, chronic patients, and the elderly. The influenza vaccine has demonstrated to be effective in these groups and is indicated in the Colombian health policy. Chronic patients with highly prevalent diseases in middle and late adulthood such as type 2 diabetes mellitus (DM) and chronic obstructive pulmonary disease (COPD) require special attention for influenza vaccination. The aim was: assess the frequency of hospitalizations and consultations related to respiratory diseases, as well as number of deaths due to pneumonia in subjects with DM or COPD, comparing those who received the influenza vaccine against those who did not receive it retrospectively over the years 2013 to 2016, and the economic impact thereof for the health system in Colombia.
Methodology:
An analysis of a retrospective cohort from 2013 to 2016 of diabetic or COPD patients was performed, assessing national information on annual vaccination coverage, and the vaccinated and unvaccinated populations were adjusted through a Propensity Score Matching (PSM) for each chronic disease, the frequency of hospitalizations, consultations due to respiratory (any etiology) and mortality rates for pneumonia. The main source of information was the Individual Health Services Provision Records (RIPS), vaccination data from the Expanded Program on Immunization (EPI) and mortality data from the National Administrative Department of Statistics (DANE). The process of analysis was validated by the Ministry of Health and Social Protection.
Results
There was a reduction of about 50% in the number of pneumonia hospitalizations of vaccinated chronic patients with DM and COPD compared to unvaccinated patients, which translates into lower economic impact costs, with potential savings of about USD $1.1 to $2.3 million for COPD, and between USD $265 thousand and USD $504 thousand for DM.
Conclusion
Vaccination against influenza in chronic patients with DM or COPD avoids hospitalizations and deaths from pneumonia, which translates into significant potential savings for the Colombian health system.
Southern Himalayas rainfall as a key driver of interannual variation of pre-monsoon aerosols over the Tibetan Plateau
Authors: Zhao Chun , Liu Weichen , Xu Mingyue , Feng Jiawang , Du Qiuyan , Gu Jun , Leung L. , Lau William 
Published: December 2022

Abstract
The Tibetan Plateau (TP) is one of the most climate sensitive regions around the world. Aerosols imported from adjacent regions reach its peak during the pre-monsoon season and play a vital role in the TP environment. However, the strong interannual variation in aerosols transported to the TP has not been fully understood. Here, we show that the interannual variability of pre-monsoon aerosols transported to the TP is influenced more by rainfall over the southern Himalayas than near-surface wind. Rainfall modulates fire events and biomass burning emissions and reduces aerosols over the TP by wet scavenging. Contrary to the role of wind in increasing aerosol transport, the positive correlation between wind and aerosols in the TP reported in previous studies is contributed by the negative interannual correlations between wind and rainfall and between rainfall and fire events over the southern Himalayas. This study highlights the co-variability of wind and rainfall and their confounding impacts on aerosols in the southern Himalayas and over the TP. With pre-monsoon rainfall projected to increase in adjacent regions of southern TP, aerosol transport to the TP may be mitigated in the future.
Synthesis of CaWO 4 as a photocatalyst for degradation of methylene blue and carmine under ultraviolet light irradiation
Authors: Yuan Cunyang , Sun Fuwei , Liu Haibo , Chen Tianhu , Chu Ziyang , Wang Hanling , Zou Xuehua , Zhai Peixun , Chen Dong 
Published: December 2022

Abstract
Due to its advantages of high efficiency, simplicity of operation, and no secondary pollution, photocatalysis is considered as a promising method for wastewater treatment. Most synthesized photocatalysts are structure complex and costly. Thus, in this study, a novel CaWO4 sample was synthesized by a co-precipitation method in one step and proved as a preferable photocatalytic material by a series of characterization methods.
The synthesized CaWO4 can be used as a catalyst to degrade dyes as methylene blue (MB) and carmine (CR), under UV light without the addition of oxidants. The effects of water matrix, including pH value, solid-liquid ratio, light intensity, and initial concentration of pollutants on photocatalytic degradation were studied. According to the results of scavengers experiments, holes and hydroxyl radicals dominate the degradation of MB while hydroxyl radical and superoxide anion are mainly responsible for the degradation of CR. Furthermore, i-t curves demonstrate the CaWO4 has good photoelectric response capability. It means that photogenerated electrons generated on the surface of the CaWO4 can form electron hole pairs, thereby forming hydroxyl radical and superoxide anion to degrade dyes. In addition, the CaWO4 has good cycling performance in the process of degrading MB. Results indicate that minerals-based photocatalyst can be synthesized in one step by simple coprecipitation and exhibit good photocatalytic effect. It provides a new idea for the study of photocatalytic degradation of dyes by mineral-like materials.
Excessive mechanical stress mediated Piezo1 activation regulates lysosomal membrane permeabilization-induced chondrocyte apoptosis in mouse osteoarthritis model
Authors: He Zili , Yan Zijian , Zhang Yu , Xv Yitie , Chen Kongbin , Zhan Jiayu , Zhang Yingze 
Published: December 2022

Abstract
Excessive mechanical stress is closely associated with progression of osteoarthritis (OA). Pathological mechanical stress can lead to chondrocyte apoptosis and subsequent cartilage degeneration. Piezo1 is a recently identified mechanosensitive ion channel that mediates chondrocyte response to mechanical stress. Here, we investigated the potential role of Piezo1 in chondrocyte apoptosis and cartilage degeneration induced by excessive mechanical stress. The primary mouse chondrocytes were subjected to mechanical stretch stress for 0, 6, 12 and 24 h, respectively. Western blot, quantitative real-time polymerase chain reaction, immunofluorescence, lysosomes and acridine orange staining were applied to examine levels of Piezo1, lysosomal function, chondrocyte apoptosis and cartilage degeneration. In vivo studies, DMM mouse model was performed to explore the protective effects and underlying mechanisms of Piezo1 inhibition on chondrocyte apoptosis and articular cartilage degradation. Exposure of chondrocytes to excessive mechanical loading caused lysosomal membrane permeabilization (LMP), which increased chondrocyte apoptosis and cartilage degeneration. Moreover, Piezo1 was upregulated in mechanical stress treated chondrocytes, and Piezo1 inhibition by siRNA or pharmacological inhibitor attenuated LMP, chondrocyte apoptosis and cartilage degeneration. We also found that Piezo1 activated cPLA2 through the calcium signaling, and cPLA2 subsequently caused the occurrence of LMP. Finally, the DMM mouse model revealed that Piezo1 inhibition reduced cPLA2 induced LMP, thereby ameliorated the surgery induced chondrocyte apoptosis and cartilage degradation, and attenuated OA pathological process. In summary, our study indicates that activity of Piezo1 was the culprit in the excessive mechanical stress-induced LMP and cartilage degradation, and Piezo1 inhibition may be a promising strategy for OA treatment.
Efficiency of modified hydrodissection vs traditional method in age related cataract surgery: a randomized clinical trial
Authors: Liu Zhen , Chen Zeli , Lan Changjun , Liao Xuan 
Published: December 2022

Abstract
Purpose
To investigate whether our modified hydrodissection is safe and efficient in age related cataract surgery.
Methods
A prospective, randomized, single-blind clinical study was performed in Chongqing Bishan Old Town Hospital from May 16 to August 31, 2022. Age related cataract patients without any other ocular disease were included. They were randomly allocated into modified hydrodissection and traditional hydrodissection groups. Hydrodissection was performed using modified or traditional method. It kept the same procedures as routine in incision, continuous circular capsulorhexis, phacoemulsification, irrigation-aspiration. Hydrodissection time and Oscar score are the main measurement to evaluate the efficiency of the modified method. Descemet membrane detachment, unstable anterior chamber and posterior capsular rupture were the main measurements to evaluate the safety.
Results
There were 156 patients (age:71.39 ± 7.17 years old; gender: 90 (57.7%) female, 66(42.3%) male) with age related cataract participating in this study. The hydrodissection time was 12.23 ± 1.84s and 18.72 ± 1.69s in the modified hydrodissection group and traditional group, respectively (P༜.001). 89.7% of eyes in modified hydrodissection group achieved Oscar score 5, which was achieved by only 75.6% eyes in traditional group (P = .033). There was no complication occurred in these patients.
Conclusions
This study demonstrated that the modified hydrodissection was as safe as traditional method but with more efficiency.
Direct use of gaseous and air-derived CO2 for amino acid biosynthesis in an ATP and NAD(P)H-free chemoenzymatic system
Authors: Liu Jianming , Zhang Han , Zeng An-Ping 
Published: December 2022

Abstract
Direct use of gaseous and air-derived CO2 for technical biosynthesis is highly desired, but elusive so far due to several obstacles including high energy (ATP, NADPH) demand, low thermodynamic driving force and limited biosynthesis rate. Here, we present an ATP and NAD(P)H-free chemoenzymatic system for amino acid biosynthesis from gaseous and air-derived CO2. It relies on a reengineered glycine cleavage system with the NAD(P)H-dependent L protein replaced by biocompatible chemical reduction of protein H with dithiothreitol. The latter provides a higher thermodynamic driving force, determines the reaction direction, and avoids protein polymerization of the rate-limiting enzyme carboxylase. Engineering of H protein to effectively release the lipoamide arm from a protected state further enhanced the system performance, achieving the synthesis of glycine, serine and pyruvate at g/L level from methanol and gaseous CO2. This work opens up the door for biosynthesis of amino acids and derived products directly from air.
Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission
Authors: Chen Pin , Lu Yanbing , He Binfeng , Xie Tao , Yan Chaolong , Liu Tengfei , Wu Silin , Yeh Yuyang , Li Zeyang , Huang Wei , Zhang Xiaobiao 
Published: December 2022

Abstract
Background and objective: The highly widespread and infiltrative nature of glioblastoma multiforme (GBM) makes complete surgical resection hard, causing high recurrence rate and poor patients’ prognosis. However, the mechanism underlying GBM migration and invasion is still unclear. In this study, we investigated the role of a Ras-related protein Rab32 on GBM and uncovered its underlying molecular and subcellular mechanisms that contributed to GBM aggressiveness.
Method: The correlation of Rab32 expression with patient prognosis and tumor grade was investigated by public dataset analysis and clinical specimen validation. The effect of Rab32 on migration and invasion of GBM had been evaluated using wound healing assay, cell invasion assay, as well as protein analysis upon Rab32 manipulations. Mitochondrial dynamics of cells upon Rab32 alterations were detected by immunofluorescence staining and western blotting. Both the subcutaneous and intracranial xenograft tumor model were utilized to evaluate the effect of Rab32 on GBM in vivo.
Results: The expression level of Rab32 is significantly elevated in the GBM, especially in the most malignant mesenchymal subtype, and is positively correlated with tumor pathological grade and poor prognosis. Knockdown of Rab32 attenuated the capability of GBM’s migration and invasion. It also suppressed the expression levels of invasion-related proteins (MMP2 and MMP9) as well as mesenchymal transition markers (N-cadherin, vimentin). Interestingly, Rab32 transported Drp1 to mitochondrial from the cytoplasm and modulated mitochondrial fission in an ERK1/2 signaling-dependent manner. Furthermore, silencing of Rab32 in vivo suppressed tumor malignancy via ERK/Drp1 axis.
Conclusion: Rab32 regulates ERK1/2/Drp1-dependent mitochondrial fission and causes mesenchymal transition, promoting migration and invasion of GBM. It serves as a novel therapeutic target for GBM, especially for the most malignant mesenchymal subtype.