bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a behavioural intervention for cognitive fatigability in multiple sclerosis: Protocol for a pilot and feasibility study
Authors: Walker Lisa A. S. , Berard Jason A. , Islam Tamanna , Pilutti Lara A. , Morrow Sarah A. , Finlayson Marcia 
Published: November 2022

BackgroundUp to 90% of people with multiple sclerosis (PwMS) subjectively report fatigue as one of their worst symptoms. Fatigability is an objectively measured component of fatigue. Cognitive fatigability (CF) is a breakdown in task performance following sustained cognitive effort. There is a paucity of interventions targeting CF in MS. The prior success of behavioural interventions at improving subjective fatigue suggests that their adaptation may yield similar results for CF. Given the relationship between CF, sleep quality, and mood, a behavioural intervention targeting these factors, such as cognitive behavioural therapy (CBT), is warranted. Given the multidimensional nature of fatigue, a multifaceted approach targeting lifestyle factors and coping (e.g., fatigue management education supplemented by CBT for insomnia and exercise) might prove efficacious.AimWe describe a protocol for a pilot feasibility study to design and implement a multi-dimensional behavioural intervention to improve CF in PwMS.MethodsStage 1: development of a multi-dimensional group-based videoconference-delivered behavioural intervention based on a previously successful fatigue management program for PwMS. A facilitator manual will be drafted. Course material will focus on four themes: body (sleep and physical activity), mood (impact of depression and anxiety), mind (cognitive contributions), and context (pacing and communication). Stage 2: a needs assessment survey will be completed by 100 PwMS for input on what factors are important contributors to their CF. Modifications will be made to the course material and manual. Stage 3: the facilitator-delivered intervention will include 20 PwMS. After baseline assessment, participants will attend weekly 70-min videoconference group sessions for 8 weeks, including homework assignments. Follow-up assessment will re-evaluate outcomes. Stage 4: analysis and dissemination of results. The primary outcome is improvement in CF. Additional feasibility outcomes will determine if a randomized control trial (RCT) is pursued. Stage 5: refine the intervention based on outcomes and feedback from participants. Determining which aspects participants felt were most effective will help inform RCT design.ConclusionThe long-term goal is to ensure that PwMS have access to effective interventions in real-world settings to improve quality of life and enhance their ability to participate in cognitively demanding activities that they enjoy.
Shifting to virtual breastfeeding counseling for low-income women in the US during COVID-19: A partner-engaged multimethod evaluation of program adaptations
Authors: Rhodes Elizabeth C. , Wilde LaPlant Helen , Zahid Mahrukh , Abuwala Nafeesa , Damio Grace , Crummett Carrianne , Surprenant Rebecca , Pérez-Escamilla Rafael 
Published: November 2022

BackgroundThe Breastfeeding Heritage and Pride program (BHP) provides evidence-based breastfeeding peer counseling to low-income women. Due to the COVID-19 pandemic, BHP shifted from delivering in-person and virtual services to providing only virtual services. Program adaptations can impact implementation success, which could influence program effectiveness. We documented program adaptations and explored their impacts on implementation outcomes, guided by the Model for Adaptation Design and Impact.MethodsThrough a community-clinical-academic partnership, we conducted in-depth interviews with 12 program implementers and peer counselors and conducted a rapid qualitative analysis. To efficiently capture information on adaptations over time, we collected and analyzed information from program meetings and extracted data from a program report. We then triangulated data from these multiple sources.ResultsPeer counselors received training on virtual service delivery and increased supportive supervision. They recruited women via phone instead of in hospitals, which was viewed as feasible. In-person counseling visits at hospitals and clients' homes were replaced with phone and video calls. Examples of changes to the content delivered included breastfeeding education in the context of the pandemic such as the latest COVID-related infant feeding guidance, provision of face masks, and more assistance with social and economic challenges. Although peer counselors increasingly adopted video calls as a substitute for in-person visits, they emphasized that in-person visits were better for relationship building, helping with breastfeeding problems like latching, and identifying barriers to breastfeeding in the home environment like limited familial support. While adaptations were reactive in that they were made in response to the unanticipated COVID-19 pandemic, most were made with clear goals and reasons such as to ensure the safety of peer counselors and clients while maintaining service delivery. Most adaptations were made through a systematic process based on program implementers' expertise and best practices for peer counseling and were largely but not fully consistent with BHP's core functions.DiscussionBHP was able to shift to virtual service delivery for continued provision of breastfeeding counseling during the pandemic. Overall, virtual services worked well but were less optimal for several aspects of counseling. Evaluations of program effectiveness of virtual services are still needed.
Sleep disturbance and problematic alcohol use: Examination of sex and race differences
Authors: Dzierzewski Joseph M. , Ravyts Scott G. , Martin Caitlin E. , Polak Kathryn M. , Nielson Spencer A. , Pomm David , Dillon Pamela , Moore Thomas B. , Thacker Leroy R. , Svikis Dace S. 
Published: November 2022

ObjectivesDisrupted sleep is prevalent and related to problematic alcohol use. While sex and race disparities exist in both sleep disturbances and problematic alcohol use, whether the association between disrupted sleep and problematic alcohol use is similar across sex and race is unknown. The present study sought to examine sex and race invariance in the association between disrupted sleep and problematic alcohol use.MethodsSecondary analyses of baseline data from a randomized clinical trial targeting heavy drinking in primary care facilities. Participants completed four individual sleep questions (sleep quality, difficulty falling asleep, difficulty staying asleep, and sleep medication use), along with sex-specific measures of problematic alcohol use (i.e., CAGE and T-ACE). A structural equation model (SEM) was used to examine the association between a latent sleep disturbance construct and problematic alcohol use, as well as potential sex and race invariance of this association.ResultsParticipants included 1,448 adults (76.59% female, 76.73% Black, Mage = 44.78, SD = 12.35). The majority of the sample reported one or more sleep disturbance symptoms and 31.84% of participants screened positive for problematic alcohol use. Greater sleep disturbance was significantly associated with a greater risk of problematic alcohol use (β = 0.18, p &lt; 0.001), and did not differ by either sex or race.ConclusionsDisrupted sleep is associated with problematic alcohol use, across sex and race. Sleep interventions may hold promise as treatment augments in individuals with problematic alcohol use.
The evaluation of different types fecal bacteria products for the treatment of recurrent Clostridium difficile associated diarrhea: A systematic review and network meta-analysis
Authors: Yang Liping , Li Wenrui , Zhang Xianzhuo , Tian Jinhui , Ma Xiaojia , Han Lulu , Wei Huaping , Meng Wenbo 
Published: November 2022

PurposeTo determine the efficacy of different types of fecal microbiota transplantation for the treatment of recurrent clostridium difficile associated diarrhea (RCDAD).MethodsWe searched PubMed, Embase, The Cochrane Library, Web of Science, China Biomedical Medicine (CBM), China National Knowledge Infrastructure (CNKI) and WanFang database. We also tracked the references found in systematic reviews of RCDAD treated with fecal microbiota transplantation. We included randomized controlled trials (RCTs) comparing different types of fecal microbiota transplantation with other methods for the treatment of RCDAD. The search period was from the date of inception of this treatment method to January 16, 2022. Two reviewers independently screened the published literature, extracted the data and assessed the risk of bias. Systematic review and network meta-analysis were conducted using RevMan 5.4, Stata 16.0 and R 4.1.2 software.ResultsTen RCTs involving 765 patients were included in this network meta-analysis. The results showed that treatment with fresh fecal bacteria and frozen fecal bacteria were better than vancomycin, fresh vs. vancomycin [odds ratio, (OR) = 8.98, 95% confidence interval (95% CI) (1.84, 43.92)], frozen vs. vancomycin [OR = 7.44, 95% CI (1.39, 39.75)]. However, there were no statistically significant differences in cure rate [fresh vs. frozen: OR = 1.21, 95% CI (0.22, 6.77); fresh vs. lyophilized, OR = 1.95, 95% CI (0.20, 19.44); frozen vs. lyophilized, OR = 1.62, 95% CI (0.30, 8.85)]. The Surface Under the Cumulative Ranking (SUCRA) indicated that fresh fecal bacteria were the best treatment for RCDAD.ConclusionsFresh fecal bacteria are the best treatment of RCDAD, frozen fecal bacteria and lyophilized fecal bacteria can achieve the same effect. Fecal microbiota transplantation is worthy of clinical and commercial application.
Development and validation of a nomogram to predict postoperative delirium in type B aortic dissection patients underwent thoracic endovascular aortic repair
Authors: Huang Wanbing , Wu Qiansheng , Zhang Yufen , Tian Chong , Huang Haishan , Wang Hui , Mao Jing 
Published: November 2022

ObjectivePostoperative delirium (POD) is a common postoperative complication after cardiovascular surgery with adverse outcomes. No prediction tools are currently available for assessing POD in the type B aortic dissection (TBAD) population. The purposes of this study were to develop and validate a nomogram for predicting POD among TBAD patients who underwent thoracic endovascular aortic repair (TEVAR).MethodsThe retrospective cohort included 631 eligible TBAD patients who underwent TEVAR from January 2019 to July 2021. 434 patients included before 2021 were in the develop set; 197 others were in the independent validation set. Least absolute shrinkage and selection operator (LASSO) and logistic regression were applied to identify the most useful predictive variables for constructing the nomogram. Discrimination and the agreement of the model was assessed with the area under the receiver operating characteristic curve (AUC), Brier score and the Hosmer-Lemeshow goodness-of-fit test. The results were validated using a bootstrap resampling and the validation set.ResultsThe incidence rate of POD observed in the development and validation cohort were 15.0% and 14.2%, respectively. Seven independent risk factors, including age ≥60 years, syncope or coma, postoperative blood transfusion, atelectasis, estimated glomerular filtration rate (eGFR) &lt;80 ml/min/1.73 m2, albumin &lt;30 g/L, and neutrophil to lymphocyte ratio, were included in the nomogram. The model showed a good discrimination with an AUC of 0.819 (95% CI, 0.762–0.876) in the developed set, and adjusted to 0.797 (95% CI, 0.735–0.849) and 0.791 (95% CI, 0.700–0.881) in the internal validation set and the external validation, respectively. Favorable calibration of the nomogram was confirmed in both the development and validation cohorts.ConclusionThe nomogram based on seven readily available predictors has sufficient validity to identify POD risk in this population. This tool may facilitate targeted initiation of POD preventive intervention for healthcare providers.
Antimicrobial Properties of Zinc Oxide Nanoparticles Synthesized from Lavandula pubescens Shoot Methanol Extract
Authors: Yagoub Abu ElGasim A. , Al-Shammari Ghedeir M. , Al-Harbi Laila Naif , Subash-Babu Pandurangan , Elsayim Rasha , Mohammed Mohammed A. , Yahya Mohammed Abdo , Fattiny Sndos Z. A. 
Published: November 2022

We report on employing in vitro biosynthesized ZnO nanoparticles using L. pubescens shoot methanol extract (50 and 100 mg LP–ZnO NPs) to examine their antimicrobial efficacy against Pseudomonas aeruginosa (ATCC27853), Staphylococcus aureus (ATCC 29213), Aspergillus niger (ATCC 16404 NA), and Aspergillus terreus (TCC 10029). The formation and stability of the investigated ZnO nanoparticles were proven by transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), UV–vis spectroscopy, X-ray diffraction (XRD), and thermal gravimetric analysis (TGA). The ZnO nanoparticles were rod-shaped (width: 10.76–30.93 nm). The nanoparticles in dimethyl sulfoxide (DMSO) outperformed their water counterparts in terms of their zones of inhibition (ZIs) (marginal means of 12.5 and 8.19 mm, respectively) and minimum inhibition concentrations (MICs) (means of 4.40 and 8.54 mg/mL, respectively). The ZI means for S. aureus, P. aeruginosa, A. terreus, and A. niger were 10.50, 6.13, 12.5, and 11.5 mm, respectively. When treating S. aureus and P. aeruginosa, the ZI of the 50 mg LP–ZnO NPs in water was better (14 mm), with a lower MIC and lower minimum bactericidal/fungicide concentrations (MBC/MFC) (7.22 and 4.88 mg/mL, respectively) than the ZnO and control drugs. The SEM images showed cellular alterations in the surface shapes after the LP–ZnO-NP treatments. Biosynthesized LP–ZnO NPs could have beneficial antibacterial properties, which could allow for future contributions to the development of new antimicrobial drugs.
Dehydration Treatment Improves Ulmus glabra Dormant Bud Regeneration from Cryostorage
Authors: Välimäki Sakari , Rusanen Mari , Aronen Tuija 
Published: November 2022

The conservation of genetic resources in cryocollections requires reliable protocols for the cryopreservation and the regeneration of the preserved material. With Ulmus glabra, the regeneration of thawed buds by in vitro organogenesis has suffered from low shoot growth and high contamination rates. The dehydration of the buds before cryopreservation improved the shoot growth rate and ameliorated the contamination rate of in vitro cultures initiated from thawed buds, although the degree of success varied depending on the donor tree.
Arabidopsis thaliana SHOOT MERISTEMLESS Substitutes for Medicago truncatula SINGLE LEAFLET1 to Form Complex Leaves and Petals
Authors: Pautot Véronique , Berbel Ana , Cayla Thibaud , Eschstruth Alexis , Adroher Bernard , Ratet Pascal , Madueño Francisco , Laufs Patrick 
Published: November 2022

LEAFY plant-specific transcription factors, which are key regulators of flower meristem identity and floral patterning, also contribute to meristem activity. Notably, in some legumes, LFY orthologs such as Medicago truncatula SINGLE LEAFLET (SGL1) are essential in maintaining an undifferentiated and proliferating fate required for leaflet formation. This function contrasts with most other species, in which leaf dissection depends on the reactivation of KNOTTED-like class I homeobox genes (KNOXI). KNOXI and SGL1 genes appear to induce leaf complexity through conserved downstream genes such as the meristematic and boundary CUP-SHAPED COTYLEDON genes. Here, we compare in M. truncatula the function of SGL1 with that of the Arabidopsis thaliana KNOXI gene, SHOOT MERISTEMLESS (AtSTM). Our data show that AtSTM can substitute for SGL1 to form complex leaves when ectopically expressed in M. truncatula. The shared function between AtSTM and SGL1 extended to the major contribution of SGL1 during floral development as ectopic AtSTM expression could promote floral organ identity gene expression in sgl1 flowers and restore sepal shape and petal formation. Together, our work reveals a function for AtSTM in floral organ identity and a higher level of interchangeability between meristematic and floral identity functions for the AtSTM and SGL1 transcription factors than previously thought.
Genome-Wide Identification and Expression Profiling of the SRS Gene Family in Melilotus albus Reveals Functions in Various Stress Conditions
Authors: Ma Biao , Nian Lili , Ain Noor ul , Liu Xuelu , Yang Yingbo , Zhu Xiaolin , Haider Fasih Ullah , Lv Ying , Bai Pengpeng , Zhang Xiaoning , Li Quanxi , Mao Zixuan , Xue Zongyang 
Published: November 2022

The plant-specific SHI-related sequence (SRS) family of transcription factors plays a vital role in growth regulation, plant development, phytohormone biosynthesis, and stress response. However, the genome-wide identification and role in the abiotic stress-related functions of the SRS gene family were not reported in white sweet clover (Melilotus albus). In this study, nine M. albus SRS genes (named MaSRS01-MaSRS09) were identified via a genome-wide search method. All nine genes were located on six out of eight chromosomes in the genome of M. albus and duplication analysis indicated eight segmentally duplicated genes in the MaSRS family. These MaSRS genes were classified into six groups based on their phylogenetic relationships. The gene structure and motif composition results indicated that MaSRS members in the same group contained analogous intron/exon and motif organizations. Further, promoter region analysis of MaSRS genes uncovered various growth, development, and stress-responsive cis-acting elements. Protein interaction networks showed that each gene has both functions of interacting with other genes and members within the family. Moreover, real-time quantitative PCR was also performed to verify the expression patterns of nine MaSRS genes in the leaves of M. albus. The results showed that nine MaSRSs were up- and down-regulated at different time points after various stress treatments, such as salinity, low-temperature, salicylic acid (SA), and methyl jasmonate (MeJA). This is the first systematic study of the M. albus SRS gene family, and it can serve as a strong foundation for further elucidation of the stress response and physiological improvement of the growth functions in M. albus.
Stipagrostis pennata (Trin.) De Winter Artificial Seed Production and Seedlings Multiplication in Temporary Immersion Bioreactors
Authors: Aghbolaghi Masoumeh Asadi , Dedicova Beata , Sharifzadeh Farzad , Omidi Mansoor , Egertsdotter Ulrika 
Published: November 2022

This study was conducted to develop the protocol for artificial seed production of Stipagrostis pennata (Trin.) De Winter via somatic embryo encapsulation as well as test a temporary bioreactor system for germination and seedling growth. Embryogenic calli were encapsulated using sodium alginate and calcium chloride and then sowed in the Murashige and Skoog (MS) germination medium in in vitro cultures. The experiments were conducted as a factorial based on a completely randomized design with three replications. The treatments include three concentrations of sodium alginate (1.5%, 2.5%, and 3.5%), two ion exchange times (20 and 30 min), and two artificial seed germination media (hormone-free MS and MS supplemented with zeatin riboside and L-proline). Germination percentage and number of days needed until the beginning of germination were studied. The highest percentage of artificial seed germination was obtained when 2.5% sodium alginate was used for 30 min (ion exchange time) and when the seeds were placed on the MS germination medium supplemented with zeatin riboside and L-proline. The results of the analysis of variance in the temporary immersion bioreactor system showed that the main effects observed on the seedling growth were associated with different growth hormones in culture media and the number of feeding cycles. Experimental results also indicated that the total protein analyses of zygotic seedlings and seedlings originating from the synthetic seeds showed no statistically significant differences between these samples.
First Evidence of Co-Circulation of Emerging Leishmania martiniquensis, Leishmania orientalis, and Crithidia sp. in Culicoides Biting Midges (Diptera: Ceratopogonidae), the Putative Vectors for Autochthonous Transmission in Southern Thailand
Authors: Songumpai Nopporn , Promrangsee Chulaluk , Noopetch Preudtipong , Siriyasatien Padet , Preativatanyou Kanok 
Published: November 2022

Since 1996, autochthonous cases of emerging leishmaniasis caused by Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis have been more frequently reported, especially in the northern and southern parts of Thailand. However, the accurate identification of their natural vectors and reservoirs remains unconfirmed. Previous studies have suggested that these emerging parasites might be transmitted by other non-phlebotomine vectors. Herein, we speculated that Culicoides biting midges might act as the competent vectors responsible for autochthonous leishmaniasis in southern Thailand. In this research, 187 non-engorged, parous and gravid Culicoides females and 47 blood-engorged ones were trapped from the residences of two recently diagnosed visceral leishmaniasis patients in Sadao District and the unaffected site in Rattaphum District, Songkhla Province, southern Thailand. Species diversity and abundance of biting midges varied among the trapping sites. Using ITS1-PCR and BLASTn analysis, L. martiniquensis was predominantly detected in several Culicoides species, including C. peregrinus, C. oxystoma, C. mahasarakhamense, and C. huffi from the vicinity of patients’ houses; and in C. fordae and C. fulvus from the unaffected site. L. orientalis was also co-circulated in C. peregrinus and C. oxystoma caught near the second patient’s house. Additionally, Crithidia sp. were also detected using SSU rRNA-PCR across Culicoides spp. Host blood meal analysis of eight different Culicoides species from the unaffected site also revealed that all trapped Culicoides had fed on cows and goats, indicating the possible role of these mammalian species as reservoir hosts. Essentially, this study is the first entomological investigation, revealing the co-circulation of emerging trypanosomatids among several species of Culicoides biting midges and strongly supporting the potential role of this insect group as the main vectors responsible for the epidemiology of autochthonous leishmaniasis in southern Thailand.
Contribuições para a constituição da história do sindicalismo docente universitário na Bolívia
Authors: Beserra Paiva Luís Roberto , Bauer Carlos 
Published: November 2022

A Bolívia é o país da América Latina no qual os princípios da reforma universitária de Córdoba foram aplicados de forma mais efetiva. Tal fato deve-se não somente à ação da destacada vanguarda estudantil, e em menor medida dos docentes e trabalhadores administrativos, mas a circunstâncias históricas que destacamos em quatro marcos temporais: a aprovação dos princípios da autonomia universitária na década de 1930, inicialmente como lei referendada (1931) e posteriormente introduzida à Constituição (1938); a Revolução Boliviana de 1952 com a ampliação da participação estudantil no cogoverno; a Revolução Universitária de 1970-1971 com a incorporação de setores operários ao cogoverno; e o processo de redemocratização entre fins da década de 1970 e início de 1980 que institucionalizou em nível nacional o cogoverno paritário e a autonomia. A principal organização sindical docente universitária, a Confederação de Docentes Universitários (CUD), foi criada em 1983, quase simultaneamente às suas congêneres na Argentina e no Brasil, mas ao contrário das entidades destes países, a CUD surge da necessidade de institucionalização em nível nacional do cogoverno. Assim, a entidade boliviana apresenta uma dupla natureza: é parte da estrutura de autogestão universitária e ao mesmo tempo representa sindicalmente os docentes universitários bolivianos. Neste texto pretendemos abordar o processo de criação, estruturação e funcionamento da CUD e, em seguida, as particularidades do processo de contrarreforma universitária neoliberal vivenciado pelos docentes bolivianos a partir da década de 1990.