bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics of Methylation of CpG Sites Associated With Systemic Lupus Erythematosus Subtypes in a Longitudinal Cohort
Authors: Lanata Cristina M. , Nititham Joanne , Taylor Kimberly E. , Solomon Olivia , Chung Sharon A. , Blazer Ashira , Trupin Laura , Katz Patricia , Dall'Era Maria , Yazdany Jinoos , Sirota Marina , Barcellos Lisa F. , Criswell Lindsey A. 
Published: May 2022

ObjectiveFindings from cross‐sectional studies have revealed associations between DNA methylation and systemic lupus erythematosus (SLE) outcomes. This study was undertaken to investigate the dynamics of DNA methylation by examining participants from an SLE longitudinal cohort using samples collected at 2 time points.MethodsA total of 101 participants from the California Lupus Epidemiology Study were included in our analysis. DNA was extracted from blood samples collected at the time of enrolment in the cohort and samples collected after 2 years and was analyzed using Illumina EPIC BeadChip kit. Paired t‐tests were used to identify genome‐wide changes which included 256 CpG sites previously found to be associated with SLE subtypes. Linear mixed models were developed to understand the relationship between DNA methylation and disease activity, medication use, and sample cell‐type proportions, adjusted for age, sex, and genetic principal components.ResultsThe majority of CpGs that were previously determined to be associated with SLE subtypes remained stable over 2 years (185 CpGs [72.3%]; t‐test false discovery rate >0.05). Compared to background genome‐wide methylation, there was an enrichment of SLE subtype–associated CpGs that changed over time (27.7% versus 0.34%). Changes in cell‐type proportions were associated with changes at 67 CpGs (P < 2.70 × 10−5), and 15 CpGs had at least 1 significant association with immunosuppressant use.ConclusionIn this longitudinal SLE cohort, we identified a subset of SLE subtype–associated CpGs that remained stable over time and may be useful as biomarkers of disease subtypes. Another subset of SLE subtype–associated CpGs changed at a higher proportion compared to the genome‐wide methylome. Additional studies are needed to understand the etiology and impact of these changes on methylation of SLE‐associated CpGs.
Machine Learning for the Identification of a Common Signature for Anti–SSA/Ro 60 Antibody Expression Across Autoimmune Diseases
Authors: Foulquier Nathan , Le Dantec Christelle , Bettacchioli Eleonore , Jamin Christophe , Alarcón‐Riquelme Marta E. , Pers Jacques‐Olivier 
Published: May 2022

ObjectiveAnti‐Ro autoantibodies are among the most frequently detected extractable nuclear antigen autoantibodies, mainly associated with primary Sjögren's syndrome (SS), systemic lupus erythematosus (SLE), and undifferentiated connective tissue disease (UCTD). This study was undertaken to determine if there is a common signature for all patients expressing anti–Ro 60 autoantibodies regardless of their disease phenotype.MethodsUsing high‐throughput multiomics data collected from the cross‐sectional cohort in the PRECISE Systemic Autoimmune Diseases (PRECISESADS) study Innovative Medicines Initiative (IMI) project (genetic, epigenomic, and transcriptomic data, combined with flow cytometry data, multiplexed cytokines, classic serology, and clinical data), we used machine learning to assess the integrated molecular profiling of 520 anti–Ro 60+ patients compared to 511 anti–Ro 60– patients with primary SS, patients with SLE, and patients with UCTD, and 279 healthy controls.ResultsThe selected clinical features for RNA‐Seq, DNA methylation, and genome‐wide association study data allowed for a clear distinction between anti–Ro 60+ and anti–Ro 60– patients. The different features selected using machine learning from the anti–Ro 60+ patients constituted specific signatures when compared to anti–Ro 60– patients and healthy controls. Remarkably, the transcript Z score of 3 genes (ATP10A, MX1, and PARP14), presenting with overexpression associated with hypomethylation and genetic variation and independently identified using the Boruta algorithm, was clearly higher in anti–Ro 60+ patients compared to anti–Ro 60– patients regardless of disease type. Our findings demonstrated that these signatures, enriched in interferon‐stimulated genes, were also found in anti–Ro 60+ patients with rheumatoid arthritis and those with systemic sclerosis and remained stable over time and were not affected by treatment.ConclusionAnti–Ro 60+ patients present with a specific inflammatory signature regardless of their disease type, suggesting that a dual therapeutic approach targeting both Ro‐associated RNAs and anti–Ro 60 autoantibodies should be considered.
Modulation of Slow Magnetic Relaxation in Gd(III)‐Tetrahalosemiquinonate Complexes
Authors: Dunstan Maja A. , Brown Dominic S. , Sorace Lorenzo , Mole Richard A. , Boskovic Colette 
Published: May 2022

AbstractIncorporating lanthanoid(III)‐radical magnetic exchange coupling is a possible route to improving the performance of lanthanoid (Ln) single‐molecule magnets (SMMs), molecular materials that exhibit slow relaxation and low temperature quantum tunnelling of the magnetization. Complexes of Gd(III) can conveniently be used as model systems to study the Ln‐radical exchange coupling, thanks to the absence of the orbital angular momentum that is present for many Ln(III) ions. Two new Gd(III)‐radical compounds of formula [Gd(18‐c‐6)X4SQ(NO3)].I3 (18‐c‐6=18‐crown‐6, X4SQ⋅−=tetrahalo‐1,2‐semiquinonate, 1: X=Cl, 2: X=Br) have been synthesized, and the presence of the dioxolene ligand in its semiquinonate form confirmed by X‐ray crystallography, UV‐Visible‐NIR spectroscopy and voltammetry. Static magnetometry and EPR spectroscopy indicate differences in the low temperature magnetic properties of the two compounds, with antiferromagnetic exchange coupling of JGd‐SQ∼−2.0 cm−1 (Hex=−2JGd‐SQ(SGdSSQ)) determined by data fitting. Interestingly, compound 1 exhibits slow magnetic relaxation in applied magnetic fields while 2 relaxes much faster, pointing to the major role of packing effects in modulating slow relaxation of the magnetization.
Recent Advances in Aptamer‐Based Nanopore Sensing at Single‐Molecule Resolution
Authors: Lv Pengrui , Zhang Wenxin , Yang Yongyi , Gao Huilin , Li Shuang , Tan Cherie S. , Ming Dong 
Published: May 2022

AbstractAptamers as the recognition element of the stochastic nanopore sensors have been under intense investigation. This paper reviews recent research advances in aptamer‐based nanopore sensing techniques, including the classification and selection of nanopores (biological nanopores, solid‐state nanopores, and nanopipettes), different strategies of aptamer‐based nanopore sensing, and their values and outlook for applications in areas such as environmental analysis, precision diagnosis, pharmaceutical industry, and security. Furthermore, the single‐molecule nanopore sensors have been applied to reveal the aptamer‐target interactions, such as recognition orientation, binding sites, and conformational heterogeneity dissociation kinetics at the single‐molecule level. In this review, recent research efforts to develop aptamer‐based single‐molecule nanopore sensors with high selectivity and sensitivity are highlighted, and some perspectives are drawn.
Rongalite as a Sulfone Source: Sulfonylation of para‐Quinone Methides and Alkyl/allyl Halides
Authors: Kale Someshwar B. , Jori Popat K. , Das Utpal 
Published: May 2022

AbstractA one‐pot three‐component reaction using p‐quinone methides, rongalite and alkyl/allyl halides has been described. The corresponding unsymmetrical sulfones were obtained in good yields under mild reaction conditions in the absence of any metal, base or any other additive.
Management of urachal cancer in pregnancy: A systematic review
Authors: Chew Kenneth K. Y. , Quah Samantha , Boot Madison R. , Sowter Steven 
Published: May 2022

AbstractUrachal cancer is a rare non‐urothelial malignancy that involves the urachus, often occurring at the junction of the urachal ligament and the bladder dome. It accounts for less than 1% of all bladder tumours. Cancer during pregnancy is rare, with the incidence of all cancers in pregnancy estimated to be 25–27 per 100 000 pregnancies. Urachal cancer in pregnancy is an even rarer phenomenon, with only a handful of case reports published to date. After a systematic review, only five cases have been reported in the English literature. We aim to review the cases presented in the literature and to examine the outcomes of the management of urachal cancer in pregnancy to date.
One‐pot sulfa‐Michael addition reactions of disulfides using a pyridine‐borane complex under blue light irradiation
Authors: Park Changhee , Lee Sunggi 
Published: May 2022

AbstractWe report a one‐pot sulfa‐Michael addition reaction using a disulfide and a pyridine‐borane complex under blue light irradiation. This novel synthetic approach has a broad substrate scope and a high functional group tolerance. Mechanistic studies suggest that sequential radical and ionic processes provide a practical solution for constructing carbon–sulfur bonds.
ESG controversies and bank risk taking
Authors: Galletta Simona , Mazzù Sebastiano 
Published: May 2022

AbstractThis study investigates how environmental, social, and governance controversies affect bank risk taking. By estimating a dynamic panel data model from 2011 to 2020, we find evidence that banks with fewer ESG controversies take less risk. Banks with a lower number of ESG controversies show their compliance with the implementation of ESG strategies to reduce risk, as evidenced by lower risk‐weighted assets and higher Z‐scores. The present study supports the recent guidelines on climate‐related and environmental risks published by the Basel Committee on Banking Supervision and the European Central Bank. Therefore, the main results strengthen the need for the integration not only of social and governance risks but also of climate‐related and environmental risks in banks' risk management framework.
High expression ofPI4K2Apredicted poor prognosis of colon adenocarcinoma (COAD) and correlated with immunity
Authors: Huang Xinkun , Cao Yang , Bao Peng , Zhu Bingye , Cheng Zhouyang 
Published: May 2022

AbstractBackgroundPI4K2A has been found to have a tumor‐promoting role in various solid tumors and be involved in various biological procedures. In this article, we aim to investigate the prognostic values of PI4K2A and provide new insights in colon adenocarcinoma (COAD).MethodsThe Cancer Genome Atlas (TCGA) database, Human Protein Atlas online database, and UALCAN database were used to analyze the expression of PI4K2A in COAD and the survival of patients. Univariate and multifactorial Cox regression analyses were used to assess the prognosis of PI4K2A on COAD. GSEA was used to explore PI4K2A‐related signaling pathways. In addition, the effect of PI4K2A on immune checkpoint inhibitors (ICIs) treatment was investigated by constructing a TIDE model and predicting the association between PI4K2A and anticancer drug sensitivity through the CellMiner database.ResultsIn the TCGA database, PI4K2A was highly expressed in COAD and the similar results were verified by qRT‐PCR. Survival analysis, utilizing Kaplan–Meier curves, revealed that COAD patients with high PI4K2A expression had a worse prognosis. In addition, PI4K2A expression was discovered to have been associated with T‐stage, N‐stage, and pathological stage by logistic analysis. Next, we utilized univariate and multifactorial Cox regression analyses to identify PI4K2A as an independent predictor. Additionally, GSEA analysis indicates that PI4K2A is enriched in MAPK signaling pathway, Toll‐like receptor signaling pathway, etc. In COAD, PI4K2A was remarkably associated with the tumor immune microenvironment. In addition, by constructing a TIDE model, we discovered that COAD patients in the PI4K2A low‐expression cohort were better treated with ICI. Finally, analysis of the CellMiner database predicted that PI4K2A was adversely correlated with the sensitivity of various anticancer drugs.ConclusionsOur study suggests that PI4K2A may be a potential predictor of poor prognosis in COAD and a potential biomarker for early diagnosis, prognosis, and treatment.
Clinical outcomes and predictors in patients with previous cardiac surgery undergoing mitral valve transcatheter edge‐to‐edge repair
Authors: De Felice Francesco , Paolucci Luca , Musto Carmine , Cifarelli Alberta , Grasso Carmelo , Tamburino Corrado , Adamo Marianna , Denti Paolo , Giordano Arturo , Bartorelli Antonio L. , Montorfano Matteo , Citro Rodolfo , Mongiardo Annalisa , Monteforte Ida , Maffeo Diego , Giannini Cristina , Crimi Gabriele , Tarantini Giuseppe , Rubbio Antonio P. , Bedogni Francesco 
Published: May 2022

AbstractBackgroundMitral‐valve transcatheter edge‐to‐edge repair (MV‐TEER) is recommended in patients with severe functional mitral regurgitation (FMR) and in those with degenerative mitral regurgitation (DMR) not eligible to traditional surgery. Patients with a history of previous cardiac surgery are considered at high risk for surgical reintervention, but data are lacking regarding procedural and clinical outcomes.Objectiveaim of this study was to assess the efficacy and clinical results of MV‐TEER in patients with previous cardiac surgery enrolled in the “multicentre Italian Society of Interventional Cardiology registry of transcatheter treatment of mitral valve regurgitation” (GIOTTO).MethodsPatients with previous coronary artery bypass grafting (CABG), surgical aortic valve replacement (AVR), or mitral valve repair (MVR) were included. Those with multiple or combined previous cardiac surgeries were excluded. Clinical follow‐up was performed at 30 days, 1 year, and 2 years. The primary endpoint was a composite of death or rehospitalization at 1‐ and 2‐year follow‐ups.ResultsA total of 330 patients enrolled in the GIOTTO registry were considered (CABG 77.9%, AVR 14.2%, and MVR 7.9%). Most patients showed FMR (66.9%), moderate reduction of left ventricular (LV) ejection fraction, and signs of LV dilation. Procedural and device successes were 94.8% and 97%. At 1 and 2 years, the composite endpoint occurred are 29.1% and 52.4%, respectively. The composite outcome rates were similar across the three subgroups of previous cardiac surgery (p = 0.928) and between the FMR and DMR subgroups (p = 0.850) at 2 years. In a multivariate analysis, residual mitral regurgitation (rMR) ≥2+ was the main predictor of adverse events at 1 year (hazard ratio: 1.54 [95% confidence interval, CI: 1.00–2.38]; p = 0.050). This association was confirmed at 2 years of Kaplan–Meier analysis (p = 0.001).ConclusionsMV‐TEER is effective in these patients, regardless of the subtype of previous cardiac surgery and the MR etiology. An rMR ≥2+ is independently associated with adverse outcomes at 1‐year follow‐up.
Development of a Prediction Model for Gas Hydrate Formation in Multiphase Pipelines by Artificial Intelligence
Authors: Sayani Jai Krishna Sahith , Sivabalan Vinayagam , Foo Khor Siak , Pedapati Srinivasa Rao , Lal Bhajan 
Published: May 2022

AbstractA prediction model is developed by means of artificial neural networks (ANNs) to determine the gas hydrate formation kinetics in multiphase gas dominant pipelines with crude oil. Experiments are conducted to determine the rate of formation and reaction kinetics of hydrates formation in multiphase systems. Based on the results, an artificial intelligence model is proposed to predict the gas hydrate formation rate in multiphase transmission pipelines. Two ANN models are suggested with single‐layer perceptron (SLP) and multilayer perceptron (MLP). The MLP shows more accurate prediction when compared to SLP. The models were predicted accurately with high prediction accuracy both for the pure and multiphase systems.
In Situ Copper Nanoparticles on Reduced Graphene Oxide (rGO/Cu) for Biphasic Hydrogen Evolution
Authors: Aslan Emre , Hatay Patir Imren 
Published: May 2022

AbstractGraphene‐based nanocomposites have attracted a tremendous attention, showing excellent performance in energy conversion applications, such as reduction of carbon dioxide and hydrogen evolution reaction (HER). Herein, a study on in situ generation Cu nanoparticles on reduced graphene oxide (rGO/Cu) by reducing simultaneously both graphene oxide and Cu2+ ions during the biphasic HER by organic sacrificial agent decamethylferrocene (DMFc) was reported. The in situ‐generated rGO/Cu catalyst was morphologically and structurally characterized by microscopic and spectroscopic techniques, respectively. The hydrogen evolution catalytic activity of rGO/Cu was investigated by using 4‐electrode voltammetry and two‐phase reaction methods. rGO/Cu nanocomposite catalyst displayed a better catalytic activity than the non‐catalyzed reaction and free‐Cu catalyst by enhancing the HER rate approximately 208‐ and 3‐times, respectively. The HER activity of rGO/Cu gave results comparable to the noble metallic and the other nanocomposite catalysts such as Pt, Pd, MoSx and their nanocomposites with carbon‐based materials.
Biolumineszenz – Teil 2: Maritime Biolumineszenz
Authors: Schramm Stefan , Weiß Dieter 
Published: May 2022

ZusammenfassungIm zweiten Teil der Serie befassen wir uns mit der maritimen Biolumineszenz. Wir erkunden die Tiefsee der Weltmeere mit ihren leuchtenden Quallen, wir begeben uns nach Japan an die Küste der Toyama‐Bay, wo jedes Jahr im späten Frühling Dinoflagellaten und Leuchtkrebse ein Schauspiel bereiten. Schließlich werden wir feststellen, dass selbst Bakterien in der Lage sind zu leuchten und diese Fähigkeit innerhalb von symbiotischen Beziehungen auch anderen Lebewesen zur Verfügung stellen. Wie bereits im ersten Teil stellen wir die Biolumineszenz der einzelnen Organismen in ihrem entdeckungs‐historischen, ökologischen und geographischen Kontext vor und beleuchten detailliert die Mechanismen der dabei ablaufenden chemischen Reaktionen.
Ein sicherer Kohlenhydratnachweis
Authors: Rautenstrauch Hanne , Rebenstorff Anne , Gudenschwager Steffen , Ruppersberg Klaus 
Published: May 2022

ZusammenfassungDie Molisch‐Probe ist ein schön anzusehender Kohlenhydratnachweis, welcher die Besonderheit aufweist, dass Pentosen von Hexosen sowie von Desoxyzuckern farblich unterschieden werden können. Der Einsatz von α‐Naphthol als Feststoff ist dabei als bedenklich anzusehen. Das Gefahrenpotenzial des Experiments kann durch einen verringerten Chemikalieneinsatz reduziert werden; außerdem kann entweder festes α‐Naphthol durch eine ethanolische Fertiglösung substituiert werden (Ersatzverfahren) oder alternativ der Naturstoff Carvacrol als Ersatzstoff verwendet werden. Unter Beachtung dieser Modifikationen ist die ‚neue‘ Molisch‐Reaktion (Carvacrol‐Reaktion) ein aussagekräftiger Schülerversuch, welcher mit einfachen Materialien umgesetzt werden kann.
SARAS: Secure resource allocation scheme for NOMA‐based device‐to‐device communication using coalition game and cognitive radio
Authors: Gupta Rajesh , Tanwar Sudeep 
Published: May 2022

SummaryDevice‐to‐device (D2D) is a fifth‐generation (5G) network's key technology which allows devices in proximity to communicate one‐to‐one without a base station (BS). Moreover, it ameliorates channel gain, communication latency, energy efficiency, and spectral efficiency. However, mitigating interference (D2D and cellular mobile users [CMUs]) and eavesdropper effect (at D2D links) is a prime concern in D2D communication. These issues can be resolved with an efficient allocation of radio resources. Cognitive radio (CR) and non‐orthogonal multiple access (NOMA) are viable solutions to resolve the aforementioned issues. Motivated by the aforementioned discussion, in this paper, we propose a joint CR and NOMA‐based scheme, that is, SARAS for secure resource allocation in D2D communication. It aims to maximize the overall sum rate and secrecy capacity of D2D users with assured quality of service (QoS) and quality of experience (QoE). CR is used for pairing between strong and weak D2D users (i.e., D2D pair) and NOMA's successive interference cancelation (SIC) technique helps to reduce the interference effect between cellular and D2D users. We used a coalition game for efficient resource utilization, which transfers D2D users from one coalition to another based on the preference order and channel conditions. This landed D2D users in the best coalition having favorable channel conditions. We have executed the proposed SARAS scheme over the MATLAB simulation tool, considering a varying number of D2D and CMUs. Simulation results show that the proposed scheme outperforms the traditional OFDMA, random, and nearest approaches concerning parameters such as sum rate and secrecy capacity.
Evaluation of advances in cortical development using model systems
Authors: Nano Patricia R. , Bhaduri Aparna 
Published: May 2022

AbstractCompared with that of even the closest primates, the human cortex displays a high degree of specialization and expansion that largely emerges developmentally. Although decades of research in the mouse and other model systems has revealed core tenets of cortical development that are well preserved across mammalian species, small deviations in transcription factor expression, novel cell types in primates and/or humans, and unique cortical architecture distinguish the human cortex. Importantly, many of the genes and signaling pathways thought to drive human‐specific cortical expansion also leave the brain vulnerable to disease, as the misregulation of these factors is highly correlated with neurodevelopmental and neuropsychiatric disorders. However, creating a comprehensive understanding of human‐specific cognition and disease remains challenging. Here, we review key stages of cortical development and highlight known or possible differences between model systems and the developing human brain. By identifying the developmental trajectories that may facilitate uniquely human traits, we highlight open questions in need of approaches to examine these processes in a human context and reveal translatable insights into human developmental disorders.
Dual‐detection approach for a charge variant analysis of monoclonal antibody combination products using imaged capillary isoelectric focusing
Authors: Candreva Jason , Esterman Abbie L. , Ge Derek , Patel Pritesh , Flagg Shannon C. , Das Tapan K. , Li Xue 
Published: May 2022

AbstractThe clinical benefits of treatments with a combination of two or more therapeutic monoclonal antibodies (mAbs) have emerged in recent years. Imaged capillary isoelectric focusing is a frequently used technology in the biopharmaceutical industry for charge variant analysis of protein therapeutics. However, with the wide concentration ranges of combination products, one component may fall within the linear detection range, whereas the other does not. Here, we report a novel methodology to explore charge variants of mAb mixtures using multiple detection techniques simultaneously. We use ultraviolet absorbance to monitor the charge variants of the high‐concentration component and native fluorescence (FL) to monitor the variants of the low‐concentration one. Charge variants of mixtures that span 40‐fold in ratio differences can be accurately quantified with this approach. In contrast to the conventional methods, it is not necessary to prepare and analyze two samples at different concentrations and combine the results for combination product testing. Additionally, the use of FL detection enables the charge variant analysis of highly potent/low abundant mAbs in a mixture. This methodology is more quality‐control friendly and efficient for the charge variant analysis of combination products with wide ratios.