bluuebunny/crossref_metadata_2025_split huggingface dataset:
Staphylococcus aureus overcomes anaerobe-derived short-chain fatty acid stress via FadX and the CodY regulon
Authors: Fletcher Joshua R. , Villareal Alex R. , Penningroth Mitchell , Hunter Ryan C. 
Published: February 2022

AbstractChronic rhinosinusitis (CRS) is characterized by immune dysfunction, mucus hypersecretion, and persistent infection of the paranasal sinuses. While Staphylococcus aureus is a primary CRS pathogen, recent sequence-based surveys have found increased relative abundances of anaerobic bacteria, suggesting that S. aureus may experience altered metabolic landscapes in CRS relative to healthy airways. To test this possibility, we characterized the growth kinetics and transcriptome of S. aureus in supernatants of the abundant CRS anaerobe Fusobacterium nucleatum. While growth was initially delayed, S. aureus ultimately grew to similar levels as in the control medium. The transcriptome was significantly affected by F. nucleatum metabolites, with the agr quorum sensing system notably repressed. Conversely, expression of fadX, encoding a putative propionate coA-transferase, was significantly increased, leading to our hypothesis that short chain fatty acids (SCFAs) produced by F. nucleatum could mediate S. aureus growth behavior and gene expression. Supplementation with propionate and butyrate, but not acetate, recapitulated delayed growth phenotypes observed in F. nucleatum supernatants. A fadX mutant was found to be more sensitive than wild type to propionate, suggesting a role for FadX in the S. aureus SCFA stress response. Interestingly, spontaneous resistance to butyrate, but not propionate, was frequently observed. Whole genome sequencing and targeted mutagenesis identified codY mutants as resistant to butyrate inhibition. Together, these data show that S. aureus physiology is dependent on its co-colonizing microbiota and metabolites they exchange, and indicate that propionate and butyrate may act on different targets in S. aureus to suppress its growth.ImportanceS. aureus is an important CRS pathogen, yet is found in the upper airways of 30-50% of people without complications. The presence of strict and facultative anaerobic bacteria in CRS sinuses has recently spurred research into bacterial interactions and how they influence S. aureus physiology and pathogenesis. We show here that propionate and butyrate produced by one such CRS anaerobe, F. nucleatum, alter the growth and gene expression of S. aureus. We show that fadX is important for S. aureus to resist propionate stress, and that the CodY regulon mediates growth in inhibitory concentrations of butyrate. This work highlights the possible complexity of S. aureus-anaerobe interactions, and implicates membrane stress as a possible mechanism influencing S. aureus behavior in CRS sinuses.
Construction of a High-Density Genetic Map Based on Large-Scale Marker Development in Coix lacryma-jobi L. Using Specifific-Locus Amplifified Fragment Sequencing (SLAF-seq)
Authors: Yang Chenglong , Ban Xiuwen , Zhou Mingqiang , Zhou Yu , Luo Kai , Yang Xiaoyu , Li Zhifang , Liu Fanzhi , Li Qing , Luo Yahong , Zhou Xiang , Lei Jing , Long Peilin , Wang Jian , Guo Jianchun 
Published: February 2022

AbstractCoix lacryma-jobi L. is one of the most important economical and medicine corn. In this study, a high genetic linkage map has been constructed for Coix lacryma-jobi L. from a cross F2 community of “Qianyi NO.2” × “Wenyi NO.2” and their parents through high-throughput sequencing and specfic-locus amplified fragment (SLAF) library construction. After pre-processing, 325.49 Gb of raw data containing 1,628 M reads were obtained. A total of 22,944 high-quality SLAFs were detected, among which 3,952 SLAFs and 3,646 of the polymorphic markers could meet the requirements for construction of a genetic linkage map. The integrated map contains 3,605 high quality SLAFs, which are grouped to 10 genetic linkage groups. The total length of the linkage map is 1,620.39 cM with an average distance of 0.45 cM and 360.5 markers in average of per linkage group. This work will provide an important molecular biology basis for investigating their characteristics, gene cloning, molecular marker-assisted breeding, functional genomics and so on for Job’s tears.
Zika virus replicates in the vagina of mice with intact interferon signaling
Authors: Lopez Cesar A. , Dulson Sarah J. , Lazear Helen M. 
Published: February 2022

ABSTRACTZika virus (ZIKV) is unusual among flaviviruses in its ability to spread between humans through sexual contact, as well as by mosquitoes. Sexual transmission has the potential to change the epidemiology and geographic range of ZIKV compared to mosquito-borne transmission and potentially could produce distinct clinical manifestations, so it is important to understand the host mechanisms that control susceptibility to sexually transmitted ZIKV. ZIKV replicates poorly in wild-type mice following subcutaneous inoculation, so most ZIKV pathogenesis studies use mice lacking IFN-αβ signaling (e.g.Ifnar1-/-). However, we found that wild-type mice support ZIKV replication following intravaginal infection, although the infection remained localized to the lower female reproductive tract. Vaginal replication was not a unique property of ZIKV, as other flaviviruses that generally are restricted in wild-type mice also were able to replicate in the vagina. Vaginal ZIKV infection required a high-progesterone state (pregnancy or pre-treatment with depot medroxyprogesterone acetate (DMPA)), identifying a key role for hormonal status in susceptibility to vaginal infection. Progesterone-mediated susceptibility did not appear to result from a compromised epithelial barrier, blunted antiviral gene induction, or changes in vaginal leukocyte populations, leaving open the mechanism by which progesterone confers susceptibility to vaginal ZIKV infection. Progesterone treatment is a key component of mouse vaginal infection models for herpes simplex virus andChlamydia, but the mechanisms by which DMPA increases susceptibility to those pathogens also remain poorly defined. Understanding how progesterone mediates susceptibility to ZIKV vaginal infection may provide insights into host mechanisms influencing susceptibility to diverse sexually transmitted pathogens.IMPORTANCEZika virus (ZIKV) is transmitted by mosquitoes, similarly to other flaviviruses. However, ZIKV is unusual in its ability also to spread through sexual transmission. We found that ZIKV was able to replicate in the vaginas of wild-type mice, even though these mice do not support ZIKV replication by other routes, suggesting that the vagina is particularly susceptible to ZIKV infection. Vaginal susceptibility was dependent on a high progesterone state, which is a common feature of mouse vaginal infection models for other pathogens, through mechanisms that have remained poorly defined. Understanding how progesterone mediates susceptibility to ZIKV vaginal infection may provide insights into host mechanisms that influence susceptibility to diverse sexually transmitted pathogens.
Tackling the integration challenge between environmental strategy and environmental management accounting
Authors: Gunarathne Nuwan , Lee Ki-Hoon , Hitigala Kaluarachchilage Pubudu K. 
Published: February 2022

PurposeThe research debate on the direct relationship between environmental strategy and environmental management accounting (EMA) is quite popular; however, integration challenges between these two factors still persist at the firm level. This paper seeks to adopt the contingency theory perspective to examine how EMA implementation varies across organizations with different intensity levels of environmental management strategy implementation (i.e. environmental management maturity, EMM).Design/methodology/approachThe paper uses a web-based survey, designed and administered to public listed companies and members of three industry chambers in Sri Lanka.FindingsThis study finds that EMA implementation is significantly different among organizations at varying EMM stages. Further, it is observed that organizations at higher stages of EMM use significantly greater domain-based EMA tools and EMA for functional purposes. Therefore, the results show that when organizations progress from reactive to proactive environmental strategies, the EMA evolves to encapsulate and diversify to deal with more-sophisticated environmental management activities.Originality/valueThis is the first study to provide cross-sectional evidence on the relationship between the application of EMA tools and functional uses and the intensity of the environmental strategy pursuance (or EMM). It also proposes a multi-item comprehensive measurement tool for EMA implementation.
A method of customer valuation score and implementation for marketing strategy
Authors: Huang Li , Liu Matthew Tingchi , Song Xi , Yen Jerome 
Published: February 2022

PurposeThis study aims to introduce a compelling customer value score method (CVSM), which is applicable for different product categories, and elaborates customer values in three components (direct economic value, depth of direct economic value and breadth of the indirect economic value) throughout three stages of customer journey.Design/methodology/approachThis study collected data from the Internet-shopping platforms, namely Taobao and T-Mall from 2019 to 2020 with particular focus on three product categories: lipstick (fast-moving consumer goods), mobile phones (durable goods) and alcohol (a hybrid of the other two product types) from 37 selected firms. The CVSM employs an entropy-based multiple criteria analysis, of which the weight of each indictor is not fixed artificially, but computed by the entropy-based method that calculates informative differences among the indicators (profit, revenue, positive reviews, search index and likes and favorites).FindingsThe result shows that product categories and market status have a moderation effect on three components in customer values. The findings suggested marketing strategies for different consumer goods, where the fast-moving consumer goods like lipstick should focus on the pre-purchase stage while the durable goods should emphasize post-purchase stage when the market is rapidly changing.Originality/valueThe study brings new insights to Kumar’s customer value theory by integrating product categories and the market status, revealing that three components of customer values differ in their contributions to the whole customer values. This paper further contributed managerial suggestions for marketers with regards to three stages of customer journey.
Storytelling in the communication of dark tourism
Authors: Tercia Christiana , Teichert Thorsten , Sirad Dini Anggraeni , Murniadi Krishnamurti 
Published: February 2022

Purpose
This study aims to tap into the storytelling’s effects of evoking personal and historical memories and their emotions on travelers’ intention to visit dark tourism sites.

Design/methodology/approach
An experimental study was performed. The authors created a story centered on dark tourism as their stimulus. The respondents received two stories in the form of printed ads. The presence and absence of a story character manipulated the stimulus. In addition to the experimental factors, four measurement constructs were included in the model: evoked historical memory, evoked personal memory, evoked emotion and intention to visit.

Findings
The results show that evoking historical and personal memories leads to traveler intention to visit the dark tourism sites whether or not the character is present or absent in the story. This study also reveals that only evoked personal memory positively affects individuals’ travel by evoking emotion. Furthermore, evoked historical memories also directly impact the evoke emotion, but only when the character is absent in the story.

Research limitations/implications
This study has three limitations. First, the measurement of emotion in this study only refers to a general measurement and does not specify between negative and positive emotions. Second, the story in the current study only focuses on one example of a natural disaster. Third, this study only used students to represent Generation Z respondents, so it would be interesting if future research compared the results across different generations.

Practical implications
The use of a reflective narrative in storytelling can be one of the options. Marketers should be cautious when using a character when it comes to dark tourism as it might have a boomerang effect, making the destination becoming unattractive to travelers, particularly, if the story tells more about the historical side of dark tourism. Managers of tourist destinations can leverage past visitors to be brand ambassadors of a place since humans share knowledge and experiences through stories and anecdotes. These personal touches can lend the personal aspects of past visitors to current ones, which can evoke memories better than an official message from a tourism board.

Originality/value
This research investigates the role of storytelling in eliciting travelers’ memories and emotional responses and how this response eventually influences their intention to visit a dark-based destination.
Thermo-hydraulic and entropy generation investigation of nano-encapsulated phase change material (NEPCM) slurry in hybrid wavy microchannel
Authors: Doshi Shivam , Kashyap Gopal , Tiwari Nishant 
Published: February 2022

PurposeThis study aims to capture the heat transfer and entropy generation characteristics of temperature-dependent nano-encapsulated phase change material (NEPCM) slurry in a hybrid wavy microchannel. In addition, the effect of substrate material combined with NEPCM slurry on conjugate heat transfer condition is captured for different microchannel heat sinks.Design/methodology/approachA novel “hybrid wavy microchannel” is proposed to enhance the overall heat transfer and reduce the pressure drop by combining wavy and raccoon geometry. NEPCM–water slurry is implied in the hybrid wavy, conventional wavy and raccoon microchannel. A user-defined function (UDF) is used to observe the effect of phase-change of paraffin material in thermophysical properties of NEPCM–water nanofluid. All three (hybrid, wavy, raccoon) microchannels are engraved on a rectangular substrate of 1.8 mm width (ωs) and 30 mm length (L), respectively. For hybrid, wavy and raccoon microchannel, waviness (γ) of 0.067 is selected for the investigation.FindingsThe result shows that NEPCM particle presence reduces the fluid domain temperature. The thermal performance of proposed Heat sink 2 is found better than the Heat sink 1. The effect of the geometrical modification, wall thermal conductivity, different volumetric concentrations of nanoparticles (ϕ ∼ 1 – 5%) and Reynolds number (Re ∼ 100 – 500) on thermodynamic irreversibility is also observed. Additionally, the effect of thermal and frictional entropy generation is reduced with a combination of NEPCM slurry and higher conductive material for all heat sinks.Practical implicationsA combination of NEPCM slurry with laminar flow microchannel cooling system emerged as a better alternative over other cooling techniques for higher power density devices such as microprocessors, electronic radar systems, aerospace applications, semiconductors, power electronics in modern electronic vehicles, high power lasers, etc.Originality/valueThe phase-change process of the NEPCM slurry is tracked under conjugate heat transfer in a hybrid wavy microchannel. Furthermore, the phase-change process of NEPCM slurry is captured with different heat sink materials (SS316, silicon and copper) under conjugate heat transfer situation for different heat sinks and concentrations (ϕ ∼ 1–5) of NEPCM.
Sustainable entrepreneurs: what drives them to engage in different crowdfunding types?
Authors: Yacoub Ghassan , Mitra Paulami , Ratinho Tiago , Fatalot Faustine 
Published: February 2022

PurposeSustainable entrepreneurs face additional challenges in fundraising. Crowdfunding is emerging as a compelling alternative to traditional sources of capital with a growing body of research outlining its characteristics and discussing mainly its antecedents. Yet little is known about the crowdfunding behaviour of sustainable entrepreneurs. The purpose of this paper is to explore sustainable entrepreneurs' crowdfunding landscape namely the relation between motivation and different crowdfunding types.Design/methodology/approachBased on a unique dataset of French sustainable entrepreneurs, this paper adopts a qualitative case study approach by conducting in-depth interviews with the entrepreneurs. This was complemented by secondary data collected from company websites and crowdfunding platforms.FindingsThis study presents a conceptual framework that outlines and maps the drivers that lead sustainable entrepreneurs to engage with different crowdfunding types (donation, reward, mixed and equity) by unravelling a more granular understanding of the key and condition-based additional drivers within the context of sustainable entrepreneurial crowdfunding.Originality/valueEntrepreneurs are taking advantage of crowdfunding in order to raise funds. This manuscript offers empirical insights into the under-investigated question of what drives sustainable entrepreneurs to engage in crowdfunding and to what ends. This research also adds texture to prior findings about general motivations of entrepreneurs to seek crowdfunding. These insights inform research and practice on sustainable entrepreneurial crowdfunding, and entrepreneurial crowdfunding in general.
An exploration of politicized healthcare access for Syrian and Palestinian refugees in Jordan: a question of equity
Authors: Halsey Kayla , Alarood Salameh , Nawaiseh Mohammed , Mir Ghazala 
Published: February 2022

Purpose
Refugees commonly face inequitable access to health care services in their host country. This study aimed to identify factors influence refugee access to health services and to assess perceptions of barriers to health care for different refugee groups in Jordan.

Design/methodology/approach
In-depth interviews were combined with document analysis and analyzed using thematic and framework methods.

Findings
Findings highlighted inequitable access to health services between different refugee groups. Unlike Palestinian refugees from the West Bank, Palestinian refugees from Gaza faced financial barriers to access health care as a result of citizenship status, which affected their health insurance, referrals for health care and legal right to work. Syrian refugees similarly lacked Jordanian citizenship and health insurance and mainly depended on UNHCR for health services, though some were able to acquire work permits and pay for private care.

Originality/value
This study demonstrates a need for politically and economically appropriate policies to minimize the disparity of health care access among these refugee groups.
Does group size and blending matter? Impact of a digital mental health game implemented with refugees in various settings
Authors: Schuler Brittany R. , Raknes Solfrid 
Published: February 2022

Purpose
There is an urgent need to upscale accessible mental health (MH) interventions that address barriers to care among Syrian refugee adolescents. The Happy Helping Hand (HH) is an innovative, cognitive behavioral digital game designed to improve MH for adolescents across digital divides. This study aims to examine the impact of HH implemented among Syrian refugees who varied according to group size and face-to-face (F2F) versus digital contact.

Design/methodology/approach
This mixed-methods study took place in Central Beqaa, Lebanon, from September 2020 to February 2021. Nine groups of Syrian displaced adolescents (n = 125) aged 13–17 years (Mage = 13.6 years) were recruited from an education center, two orphanages and one informal settlement to participate in the ten-session HH program. The WHO-Five-Well-being Index (WHO5) is a validated measure used to evaluate HH impact on MH at baseline and directly postintervention.

Findings
Significant improvements were seen in WHO5 scores in F2F and digital settings from baseline to follow-up. At baseline, 28% reported normal well-being, which increased to 99% after HH participation. WHO5 scores changed from M = 59.4 at baseline, indicating depression, to M = 77.3 at follow-up, indicating normal well-being. Smaller groups with more F2F contact reported greater improvements in mean WHO5 than larger groups with less F2F contact. The greatest aggregate change in well-being was achieved when HH was implemented digitally in bigger groups.

Originality/value
Study results indicate that the HH game can improve well-being and MH for Syrian refugees. Importantly, results build on the base of evidence on digital MH interventions as promising tools on the way to ensure healthy lives and well-being for all.
A review of ontologies for augmented reality cultural heritage applications
Authors: Vlachos Apostolos , Perifanou Maria , Economides Anastasios A. 
Published: February 2022

PurposeThe purpose of this paper is to review ontologies and data models currently in use for augmented reality (AR) applications, in the cultural heritage (CH) domain, specifically in an urban environment. The aim is to see the current trends in ontologies and data models used and investigate their applications in real world scenarios. Some special cases of applications or ontologies are also discussed, as being interesting enough to merit special consideration.Design/methodology/approachA search using Google Scholar, Scopus, ScienceDirect and IEEE Xplore was done in order to find articles that describe ontologies and data models in AR CH applications. The authors identified the articles that analyze the use of ontologies and/or data models, as well as articles that were deemed to be of special interest.FindingsThis review found that CIDOC-CRM is the most popular ontology closely followed by Historical Context Ontology (HiCO). Also, a combination of current ontologies seems to be the most complete way to fully describe a CH object or site. A layered ontology model is suggested, which can be expanded according to the specific project.Originality/valueThis study provides an overview of ontologies and data models for AR CH applications in urban environments. There are several ontologies currently in use in the CH domain, with none having been universally adopted, while new ontologies or extensions to existing ones are being created, in the attempt to fully describe a CH object or site. Also, this study suggests a combination of popular ontologies in a multi-layer model.