bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trimester specific determinants of Pregnancy loss among women in Reproductive age group from India -Evidence from National Family Health Survey (2015-16)
Authors: Das Milan , Singh Mayank , Garg Priyanka , Rizvi Ali Abbas 
Published: April 2022

Abstract
Background: Abortions are the most common causes of pregnancy loss among women of reproductive age. In a country like India, there is not enough effort being done to address this problem.Methods: The National Family Health Survey (2015-16) was used in this study, and bivariate and multivariate logistic regression analysis were used. The GIS map is used to understand the spatial distribution of abortions.Results: Our results show that the rates of abortions rise with women's age, education, and place of residency, according to our findings. Muslim women have a lower risk of abortion than Hindu women. The rich women have a higher chance of having an abortion than the poor women. The socioeconomic characteristics were also linked to the loss of pregnancy.Conclusions: Abortion care for women that is both affordable and well-informed can improve women's reproductive health. In the backward districts, the government should strengthen the use of contraceptives.
Sustainable production of hydrogen with high purity from methanol and water at low temperatures
Authors: Zhang Sai , Liu Yuxuan , Zhang Mingkai , Ma Yuanyuan , Hu Jun , Qu Yongquan 
Published: April 2022

Abstract
Carbon neutrality initiative has stimulated the development of the sustainable methodologies for hydrogen generation and safe storage. Aqueous-phase reforming methanol and H2O (APRM) has attracted the particular interests for their high gravimetric density and easy availability. Thus, to efficiently release hydrogen and significantly suppress CO generation at low temperatures without any additives is the sustainable pursuit of APRM. Herein, we demonstrate that the dual-active sites of Pt single-atoms and frustrated Lewis pairs (FLPs) on porous nanorods of CeO2 enable the efficient additive-free H2 generation with a low CO generation (0.027 %) through APRM at 120 °C. Mechanism investigations illustrate that Pt single-atoms and Lewis acidic sites cooperatively promote the activation of methanol. With the help of a spontaneous water dissociation on FLPs, Pt single-atoms exhibit a significantly improved reforming of *CO to promote H2 production and suppress CO generation. This finding provides a promising path towards the flexible hydrogen utilizations.
Artificial neural network as a tool for estimation of the higher heating value of miscanthus based on ultimate analysis
Authors: Brandić Ivan , Pezo Lato , Bilandžija Nikola , Peter Anamarija , Šurić Jona , Voća Neven 
Published: April 2022

Abstract
Miscanthus is a perennial energy crop that produces high yields and has the potential to be converted into energy. The ultimate analysis determines the composition of the biomass and the energy value in terms of the higher heating value (HHV), which is the most important parameter in determining the quality of the fuel. In this study, an artificial neural network (ANN) model based on the principle of supervised learning was developed to predict the HHV of miscanthus biomass. The developed ANN model was compared with the models of predictive correlations (suggested from the literature) and the accuracy of the developed model was determined by the correlation coefficient. The paper presents data from 192 miscanthus biomass samples based on ultimate analysis and HHV. The developed model showed good properties and the possibility of prediction with high accuracy (r2 = 0.975). The paper proves the possibility of using ANN models in practical application in determining fuel properties of biomass energy crops and greater accuracy in predicting HHV than the correlation models offered.
Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome
Authors: Song Yali , Ye Wenting , Xie Tingting , Yang Yi , Luo Xiaoqing , Zhou Lili 
Published: April 2022

Abstract
Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disease related to infertility in women. IVF-ET is the effective way to treat infertility in PCOS patients. But poor oocytes quality of PCOS patients results in low pregnancy rate and the underlying mechanism is unknown. Klotho, an anti-aging factor, was reported up-regulated in PCOS and related to excessive androgens. However, the role of Klotho in poor oocytes quality of PCOS has not been clarified. Method: We enrolled 72 PCOS and 70 healthy participants who underwent IVF-ET and the follicular fluid was collected. In addition, granulosa cells from 15 patients underwent IVF-ET were collected. The level Klotho in follicular fluid was tested by ELISA. The mRNA level of Caspase-3, Caspase-9, IL-6, TNF, PCNA and GDF9 in granulosa cell were detected by qRT-PCR.Results: Compared with the control group, the level of Klotho, Caspase-3, Caspase-9, IL-6 and TNF in PCOS group was significantly higher, especially in the group of PCOS with hyperandrogenism, while the level of PCNA and GDF9 was down-regulated. Correlation analysis shown that level of Klotho in follicular fluid of PCOS group was negatively correlated with the number of mature oocytes. Similarly, we divided the PCOS group into two groups according to the median of Klotho level and found that the number of mature oocytes is significantly lower in the group of high level of Klotho. In addition, the Klotho level in follicular fluid was also positively correlated with serum testosterone, LH, LH/FSH, menstrual cycle and number of total antral follicles in the group of PCOS. Conclusions: These data suggested that Klotho may act as a new biomarker evaluating the quality of oocytes in patients with PCOS, indicating the severity of PCOS and the outcome of IVF in PCOS patients.
Complete genome sequence and phylogenetic analysis of a novel orthonairovirus from ticks in China
Authors: Li Hangyuan , Shao Jianwei , Pei Mingchao , Cao Chong , Sun Mingfei , Zhang Xu 
Published: April 2022

Abstract
The complete genome sequence of a novel tick carried orthonairovirus, Meihua Mountain virus (MHMV), is presented for the first time. With unbiased high-throughput sequencing and Sanger sequencing, a novel Nairobi sheep disease (NSD) genogroup orthonairovirus, MHMV, was identified in bloodsucking ticks collected from cattle and wild boar in Fujian province, Southeastern China. Genomic architecture of MHMV shares typical features with orthonairoviruses. Phylogenetic analyses suggested that MHMV is closely related to Hazara virus and is clustered into the NSD genogroup of the genus Orthonairovirus. The RNA-dependent RNA polymerase (RdRp), glycoprotein precursor (GPC), and nucleoprotein (N) of MHMV share 62.3–83.5%, 37.1–66.1%, and 53.4–77.3% amino acid identity with other NSD genogroup viruses, respectively, representing a novel species. The overall pooled prevalence of MHMV in ticks was 2.54% (22/134 tick pools). This study presented the first report of tick-carried orthonairovirus in Fujian province and highlighted the broad geographic distribution and high genetic diversity of orthonairoviruses in China.
Contrasting the diversity patterns and processes of microbial community assembly in water and sediment in Lake Wuchang, China
Authors: Yang Deguo , Li Xuemei , Meng Zihao , Chen Kang , Hu Feifei , Liu Lu , Zhu Tingbing 
Published: April 2022

Abstract
Background: Microorganisms are an integral component of lake ecosystems, as they play crucial roles in material circulation and energy flow. Understanding the distribution of bacterial communities, their mechanisms of formation, and the ecological functions of such communities in lakes is essential for the diagnosis and management of lake ecosystems.Results: We explored the diversity patterns and processes of microbial community assembly in the water and sediment of Lake Wuchang (China) using high-throughput sequencing of 16S rRNA gene amplicons on the Illumina platform. The results revealed a higher microbial α-diversity in the sediment than in water (P < 0.01), and the most common bacterial phyla in the water column were Proteobacteria (average 39.19%), Cyanobacteria (24.12%), and Actinobacteria (15.73%), while the dominant bacterial phylum in the sediment was Proteobacteria (38.90%), followed by Acidobacteria (9.84%), Chloroflexi (8.93%), and Nitrospirae (8.61%). Through functional annotation, functions related to phototrophy and nitrogen metabolism primarily occurred in the water column and sediment, respectively. Unconstrained principal coordinates analysis (PCoA) showed that in water samples, the microbial communities from different seasons were divided into three groups, while samples collected from sediment revealed no such dispersion. These results were supported by the analysis of similarities, as the difference between season groups in water was greater than between groups in sediment (R = 0.759, p = 0.001 and R = −0.012, p = 0.488, respectively). According to Pearson correlation analysis, different environmental variables showed significant roles in shaping the microbial communities, as water temperature, dissolved oxygen, water depth, total nitrogen, ammonium, and nitrite were key factors for microbial community structure in the water column, while TN in sediment, conductivity, and organic matter were key factors in sediment. However, we found that stochastic processes (the value of |βNTI| < 2) dominated community assembly in both the water column and sediment of Lake Wuchang. Conclusions: This study represents one attempt to explore the microbial diversity patterns and the community assembly mechanisms in lake ecosystems and the influences of sampling scale. The results will provide a foundation for microbial development and utilization in lake water column and sediment under the circumstances of increasing tendency of lake ecological fishery.
The white star apple (Chrysophyllum albidum G. Don), a revealed potential pan tropical forest food species in the context of climate and global changes
Authors: GANGLO Jean Cossi 
Published: April 2022

Abstract
Chrysophyllum albidum is a forest food tree species of the Sapotaceae family bearing large berries of nutrition, sanitary, and commercial values in many African countries. It naturally grows in lowland rain forest and is widely distributed in West, Central, and East Africa. Because of its socio-economic importance, C. albidum is threatened at least by human pressure. However, we do neither know at which extent climate change can impact its distribution, nor whether it is possible to introduce the species in other tropical regions. In order to resolve our concerns we decided to model the spatial distribution of the species. We then used the SDM package for data modelling in R to compare predictive performances of algorithms among the most common used: three machine learning algorithms (MaxEnt, Boosted Regression Trees, and Random Forests) and three regression algorithms (Generalized Linear Model, Generalized Additive Models, and Multivariate Adaptive Regression Spline). We performed model transfers in tropical Asia and Latin America. At the scale of Africa, predictions with respect to Maxent, under Africlim (scenarios rcp 4.5 and rcp 8.5, horizon 2055) and MIROCES2L (scenarios SSP245 and SSP585, horizon 2060) showed that the favorable areas of C. albidum will extend mostly in West, East, Central, and Southern Africa as well as in East Madagascar. As opposed to Maxent, in Africa, the predictions of BRT and RF were unrealistic with respect to the known ecology of C. albidum. All the algorithms were consistent in predicting a successful introduction of C. albidum in Latin America, both at present and in the future. Predictions of spatial distribution of C. albidum at present and in future under MIROCES2L, scenarios SSP245 and SSP585, horizon 2060 were coherent and even complementary between BRT and RF and showed that the species can be successfully introduced in tropical Asia.
Prognostic value of pericardial effusion in patients with STEMI treated with a pharmacoinvasive or a primary percutaneous intervention: From an experience of a tertiary center
Authors: Kiris Tuncay , Esin Fatma kayaltı , Avci Eyüp , Celik Aykan , Özkan Emel , Bayata Serdar , Karaca Mustafa 
Published: April 2022

Abstract
Background: The long-term prognostic relevance of pericardial effusion (PE) in ST-segment elevation myocardial infarction (STEMI) patients treated with a pharmacoinvasive or a primary percutaneous intervention (PCI ) strategy are not well established. The aim of this study was to investigate the prognostic impact of pericardial effusion in these patients.Methods: A total of 860 STEMI patients underwent a pharmacoinvasive or a primary PCI strategy were enrolled in this study. Echocardiographic, demographic, and laboratory data were collected. All-cause mortality was the primary end-point.Results: PE was present in 51 patients (5.9%). The patients with PE was higher long-term mortality rate compared with those did not (41% vs 27%, p = 0.0025). However, both multivarite analysis (HR:1.243, 95%CI: 0.765-2.022, p = 0.380) and propencity matching scores (40% vs 28%, p = 0.205) showed that there was no relationship between PE and long-term mortality.Conclusion: The presence of PE was not independent predictor of long-term mortality in STEMI patients treated with a pharmacoinvasive or a PCI.
Mesenchymal Stem Cells Derived from Umbilical Cord Blood Having Excellent Stemness Properties with Therapeutic Benefits - a New Era in Cancer Treatment
Authors: Ray Suman Kumar , Mukherjee Sukhes 
Published: April 2022

Abstract:Mesenchymal stem cells (MSCs) are the most promising candidates for cellular therapies, and most therapeutic applications have focused on MSCs produced from adult bone marrow, despite mounting evidence that MSCs are present in a wide range of conditions. Umbilical cord blood (UCB) is a valuable source of hematopoietic stem cells, but its therapeutic potential extends beyond the hematopoietic component, which also suggests solid organ regenerative potential. With potential ranging from embryonic- like to lineage-committed progenitor cells, many different stems and progenitor cell populations have been postulated. MSC is currently inferred by numerous clinical applications for human UCB. As stem cell therapy kicks off some new research and these cells show such a boon to stem cell therapy, it is nevertheless characteristic that the prospect of UCB conservation is gaining momentum. Taken together, the experience described here shows that MSCs derived from UCB are seen as attractive therapeutic candidates for various human disorders including cancer. It is argued that a therapeutic stem cell transplant, using stem cells from UCB, provides a reliable repository of early precursor cells that can be useful in a large number of different conditions, considering issues of safety, availability, transplant methodology, rejection, and side effects. In particular, we focus on the concept of isolation and expansion, comparing the phenotype with MSC derived from the UCB, describing the ability to differentiate, and lastly, the therapeutic potential concerning stromal support, stemness characteristic, immune modulation, and cancer stem cell therapy. Thus it is an overview of the therapeutic application of UCB derived MSCs, with a special emphasis on cancer. Besides, the current evidence on the double-edged sword of MSCs in cancer treatment and the latest advances in UCB-derived MSC in cancer research will be discussed.
Synthesis and Antitumor Evaluation of Glutathione Responsive Self-Immolative
Disulphide Linked Camptothecin-Biotin Conjugate
Authors: Kaur Amardeep , Dhiman Shikha , Lee Hong Boon , Sharma Manu 
Published: April 2022

Background:
Camptothecin is a naturally occurring alkaloid obtained from the stem wood of the Chinese
tree, Camptotheca acuminata. It exerts pharmacological effects due to its ability to selectively inhibit the type-I topoisomerase
DNA nuclear enzyme. Several semisynthetic analogs of camptothecin have been synthesized to date possessing
antitumor activity.

Objective:
Camptothecin (CPT) is one of the most promising anticancer drugs but it produces various side effects
because of its non-selectivity towards cancer cells. To overcome these adverse effects, we synthesized biotin conjugate
of camptothecin, which was linked via a self-immolative disulfide linker (CPT-SS-Biotin).

Method:
Biotin conjugated camptothecin linked through a disulfide bond was synthesized following schemes, and the
structural characterization was carried out. The stability and drug release studies were performed in the presence of
glutathione (GSH) while in vitro studies were performed on 4T1 tumor cell lines. In vivo pharmacological investigation
was done using an antitumor Wistar rat model.

Results:
The stability and drug release studies were performed in the presence of glutathione (GSH), and CPT-SSBiotin
was found to be physiologically stable moiety and can only be cleaved in the presence of GSH to release free
CPT. The CPT-SS-Biotin showed higher toxicity in the biotin-overexpressing 4T1 tumor cell line with a lower IC50
value (8.44 μM) compared to camptothecin alone (IC50 > 30 μM). CPT-SS-Biotin also showed 10.6% higher cellular
uptake by cells in comparison to free camptothecin. The CPT-SS-Biotin was delivered to cells by binding to the biotin
receptors on the cell surface, followed by energy-dependent endocytosis and internalization to cause cellular toxicity.

Conclusion:
In-vivo tumor suppression studies and in vitro cell line studies along with serological parameters and
histopathological studies showed that conjugate produced a high therapeutic effect and remarkably reduced toxic effects
in comparison to free CPT. The results suggested that biotinylation of camptothecin via disulfide linker can be a
safe and efficacious method in cancer therapeutics.
‘Convalescent Plasma’- An Effective Treatment Option to Prevent Emerging nCOVID-19- A Review
Authors: Ashique Sumel , Khatun Tahamina , Sahu Garima , Upadhyay Aakash , Adhana Archana , Kumar Shubneesh , Sandhu Navjot , Kumar Nitish 
Published: April 2022

Abstract:To date, no accepted therapy exists for treating the emerging infectious disease nCOVID-19 in a safe and effective way. Although various types of treatment options are under the developing phase around the world, among those, several studies concerning convalescent plasma (CP) from recovered patients reported promising effects against nCOVID-19 infected critically ill patients nowadays. The therapy showed very few adverse effects, and it helps to diminish the viral load when administered at an early stage of infection. Convalescent plasma appears to be safe for critically ill nCOVID-19 patients by decreasing serum viral loads, and most cases are virus negative after CP transfusion. The convalescent plasma can be good as it has already been utilized to treat previous pandemics like Ebola, influenza, and SARS-CoV infection. In this review article, we pointed out the background, rationality, mechanisms, and safety outcomes of CP to treat nCOVID-19 infected patients.
A study of physical fitness characteristics of junior wushu players depend on sex difference
Authors: Sukamti Endang Rini , Budiarti Ratna , Nugroho Wisnu , Miftachurochmah Yulvia , Azeem Kaukab 
Published: April 2022

Physical fitness was an activity or exercise that can accelerate regeneration when performing several exercises or training. This study aimed to examine the physical fitness characteristics of male and female junior wushu players, followed by determining physical performance differences between both groups. The analytical observational study method was implemented to conduct this study using a cross-sectional approach Fifty junior wushu players (male: n= 25, height= 147.78 ± 12.96 cm; female: n= 25, height= 147.78 ± 12.60 cm) participated in this study by conducting some physical fitness tests. The results showed significant differences in the indicators of 20-meters running with a P-value of 0.049, backbend with a P-value of 0.000, and vertical jump P-value of 0.021. Meanwhile, there was no significant difference between male and female wushu players in the T-test, push up, sit up, dan sit reach (P0,05). From the results, it can be said that male players have higher scores in terms of weight, height, speed, and power, while female players have higher scores in terms of flexibility. This study concluded that it can be taken into consideration to optimize training and testing procedures for junior wushu players.
The Acceptability of Adherence Support via Mobile Phones for Antituberculosis Treatment in South India: Exploratory Study
Authors: Jose Nisha K , Vaz Clint , Chai Peter R , Rodrigues Rashmi 
Published: April 2022

Background
India has the greatest burden of tuberculosis (TB). However, over 15% of the people on antitubercular therapy (ATT) in India are nonadherent. Several adherence monitoring techniques deployed in India to enhance ATT adherence have had modest effects. Increased adoption of mobile phones and other technologies pose potential solutions to measuring and intervening in ATT adherence. Several technology-based interventions around ATT adherence have been demonstrated in other countries.

Objective
The objective of our study was to understand the acceptance of mobile phone adherence supports for ATT using self-administered quantitative measures among patients with TB in South India.

Methods
This exploratory study was conducted at a TB treatment center (TTC) at a tertiary care center in Thrissur District, Kerala, India. We recruited 100 patients with TB on ATT using convenience sampling after obtaining written informed consent. Trained study staff administered the questionnaire in Malayalam, commonly spoken in Kerala, India. We used frequency, mean, median, and SD or IQR to describe the data.

Results
Of the 100 participants diagnosed with TB on ATT, 90% used mobile phones routinely, and 84% owned a mobile phone. Ninety-five percent of participants knew how to use the calling function, while 65% of them did not know how to use the SMS function on their mobile phone. Overall, 89% of the participants did not consider mobile phone–based ATT adherence interventions an intrusion in their privacy, and 93% did not fear stigma if the adherence reminder was received by someone else. Most (95%) of the study participants preferred mobile phone reminders instead of directly observed treatment, short-course. Voice calls (n=80, 80%) were the more preferred reminder modality than SMS reminders (n=5, 5%).

Conclusions
Mobile phones are likely an acceptable platform to deliver ATT adherence interventions among individuals with TB in South India. Preference of voice call reminders may inform the architecture of future adherence interventions surrounding ATT in South India.