bluuebunny/crossref_metadata_2025_split huggingface dataset:
School and community driven dengue vector control and monitoring in Myanmar: Study protocol for a cluster randomized controlled trial
Authors: Overgaard Hans J , Linn Nay Yi Yi , Kyaw Aye Mon Mon , Braack Leo , Tin Myo Win , Bastien Sheri , Velde Fiona Vande , Echaubard Pierre , Zaw Win , Mukaka Mavuto , Maude Richard J 
Published: July 2022

Abstract
BackgroundDengue is the most common and widespread mosquito-borne arboviral disease globally estimated to cause >390 million infections and >20,000 deaths annually. There are no effective vaccines or preventive drugs. Control of dengue transmission relies primarily on mosquito vector control. Although most vector control methods currently used by national dengue control programs may temporarily reduce mosquito populations, there is little evidence that they affect transmission. There is an urgent need for innovative, participatory, effective and locally adapted approaches for sustainable vector control and monitoring in which students can be particularly relevant contributors and to demonstrate a clear link between vector reduction and dengue transmission reduction, using tools that are inexpensive and easy to use by local communities in a sustainable manner.MethodsHere we describe a cluster randomized controlled trial to be conducted in 46 school catchment areas in two townships in Yangon, Myanmar. The outcome measures are dengue cases confirmed by rapid diagnostic test in the townships, dengue incidence in schools, entomological indices, knowledge, attitudes and practice, behavior and engagement.Discussion The trial involves middle school students that positions them to become actors in dengue knowledge transfer to their communities and take a leadership role in the delivery of vector control interventions and monitoring methods. Following this rationale, we believe that students can become change agents of decentralized vector surveillance and agents of sustainable disease control in line with recent new paradigms in integrated and participatory vector surveillance and control. This provides an opportunity to operationalize transdisciplinary research towards sustainable health development.Trial registrationISRCTN78254298, Registered 31 May 2022, https://www.isrctn.com/ISRCTN78254298Sponsor: Norwegian University of Life Sciences (NMBU)
Temperature impacts on residential electricity consumption in Anhui, China: Does the electricity price matter?
Authors: Li Lanlan , Song Xinpei , Li Jingjing , Li Ke , Jiao Jianling 
Published: July 2022

Abstract
Global warming leads to the problem of climate adaptability, which makes residents' electricity consumption behavior more sensitive to temperature. Understanding the shape of the temperature–electricity consumption response curve is helpful for planning power investment and production, and to facilitate a green and low-carbon transformation of the power system. Using data regarding electricity consumption in nearly 20,000 households from seven cities in Anhui Province, China, from 2016–2017, this study examined the response of residential electricity consumption to temperature. The results show that although residential electricity consumption was proportional to the average temperature, the variation range of the residential electricity consumption was not the same in each percentile range of temperature. In particular, under the possible influence of the electricity price and weather factor, the residential electricity consumption temperature response curve has a "V"-shape when the average temperature is over 30°C. The heterogeneity analysis shows that the temperature and electricity response curve have strong fluctuations under the change of the time-of-use (TOU) pricing policy. This implies that the price policy helps to regulate the power consumption temperature response curve and thus has an impact on the power load.
The importance of understanding the local community’s attitude toward nature conservation
Authors: Obradović Sanja , Stojanović Vladimir , Milić Dubravka 
Published: July 2022

Abstract
Protected areas are an important cornerstone in trying to halt habitat and species loss, but it is generally accepted that conservation policies must also favor the local community as an integral part of these areas. This study reveals the importance of understanding local residents' perception of protected areas’ impacts on their livelihoods and development. It analyzes the perceptions of 612 respondents living in 15 settlements surrounding two protected areas in Serbia. The findings reveal that the locals have a very positive attitude about the impact of protected areas through benefits and protection of resources. They have a very strong sense of belonging to the place and are willing to contribute to protection, but they feel excluded, which is worrisome. A different approach of management that would respect the rights and the existence of the local people is required to prevent conflicts and foster mutual respect and benefit-sharing for everyone.
Experimental study on the cytocompatibility of milling surface of Poly-ether-ether-ketone (PEEK)
Authors: Liu Xinrui , Lei Qian 
Published: July 2022

Abstract
Cytocompatibility of implant material is dependent on the processed surface topography. This study aimed to characterize the milling surface topography features and to assess the cytotoxicity of PEEK processed using end milling technology. Based on the Taguchi method, end milling experiments have been conducted on PEEK to characterize surface topographies induced by various milling conditions. Machined surface defects and 3-Dimensional surface topography parameters such as surface average height Sa, the ten-point height of surface Sz, Skewness Ssk, and Kurtosis Sku were characterized. The relationships between the cutting technological parameters and surface topography parameters were established by developing a linear regression model. On the other hand, the effect of the machined surface topography on the viability of fibroblasts was investigated by using cells from a mouse fibroblast L929 and the agar overlay test, MTT test. The experimental result indicated that feed marks, scratch marks, budlike protuberances, the adhered surface particles are the main forms of the surface defect in the end milling of PEEK. The axial and radial depths of cut are the two most critical factors to affect surface topography parameters. Agar overlay tests produced no evidence of cell damage caused by PEEK, and a conclusion can be drawn that PEEK has no cytotoxicity to L929 cell. MTT test results indicated that milling-induced surface topography features exerted specific toxicity on L929 cells, and a statistical difference between the cell’s viability of the test samples is observable. By correlation analysis, the correlations order of the viability of fibroblasts L929 with the machined surface roughness parameters were Ssk, Sa, Sku and Sz in large to small. It is concluded that end milling processing significantly affects surface topography which in turn influences the L929 fibroblast cellular response. A significant decrease of L929 viability started with the surface roughness of Sa = 1.18 μm, and surface roughness characterized by Ssk = 0.011 (namely, Ssk closed to zero) also allowed to achieve the highest possible cells viability.
The Long Shadow of History? The Impact of Colonial Labor Institutions on Economic Development in Peru
Authors: Abad Leticia Arroyo , Maurer Noel 
Published: July 2022

Abstract

How long is the shadow of history in explaining economic outcomes? We examine a canonical case of forced labor: the mita and the encomienda in colonial Peru. The mita was a labor draft designed to provide workers for mines, churches, and public works in colonial Peru and Bolivia. The encomienda granted Spanish conquistadores the right to extract resources from indigenous peoples. We examine the impact of forced labor using a dataset of 500 indigenous settlements scattered across modern-day Peru. We find that forced labor gravely impacted the Peruvian communities subjected to it, but the effects dissipated before the end of the colonial period (1532-1811). We test for a possible "reversal of fortune" in the postcolonial period by looking at education and access to land for the 19th and 20th centuries and find no significant differences. The results hold when we examine the mita and encomienda separately. While forced labor mattered, its effects did not persist. It appears the shadow of history was not that long after all.
Geographic transitions of domestic cats in urban areas through animal adoption centers and the implications for population dynamics
Authors: Flockhart D. T. Tyler , Moore Sophie M. , Decker Samuel T. , Kilgour R. Julia 
Published: July 2022

Abstract
Animal shelters address animal welfare in communities through the intake and outcome of companion animals, but these efforts affect population dynamics of companion animals based on the distance animals are moved and the factors that underlie intake and outcome. Using data from an animal shelter in Washington, D.C., we analyzed cat intakes and outcomes based on geographic and socioeconomic factors. Most intakes were stray cats (59%) and cats relinquished by owners (38%) whereas most outcomes were adoptions (84%). The highest number of intakes were in high development, low-income neighborhoods whereas the lowest number of intakes were in low development, high-income neighborhoods. The highest number of outcomes were to high-income neighborhoods and there was a trend towards more outcomes in neighborhoods further from the shelter. Cats returned to the shelter were more likely to originate nearer the shelter compared to relinquishment. Stray intakes were less common, and return to shelter were more common, in high-income, high development areas. Seized cats originated from low-income neighborhoods. Relative to adoptions, the proportion of returned to owner outcomes was higher in low-income neighborhoods that were closer to the shelter as well as high-income neighborhoods that were distant the shelter. Our results highlight the factors underlying cat intakes and outcomes in shelters that ultimately determine where, when, and how animals are moved across urban areas; these factors must be considered when developing cat population management plans to reach animal welfare and societal goals.
Optimization of Array System Configuration Using the Smoothed-Pad Algorithm
Authors: Batubara Mario , Manik Timbul , Sitompul Peberlin , Lathif Muthofa 
Published: July 2022

Abstract
The source of astronomical objects, in general, is a very far distance from the observation instrument so that the transmitted signal is received very small and is at risk of being disturbed by other sources around the receiving system. Therefore, the concept of multiple receivers with array interferometry configuration arrays is used to improve the quality of the source signal detected by the receiving system. The position of each receiving system, known as the antenna pad, determines the quality performance of the received signal output. The antenna positions are arranged in a discrete grid system so that there is a vacancy between antenna pads. In this paper, a computational technique is present to overcome the region’s emptiness between one antenna to its neighbor. As an initial result, the integrating of each position with its neighboring pads with some of its smoothing functions is optimal in filling the void region and maximizing the u-v coverage.
Single-Cell RNA-seq Uncovers Dynamic Processes Orchestrated by RNA-Binding Protein DDX43 in Chromatin Remodeling during Spermiogenesis
Authors: Zheng Ke , Tan Huanhuan , Wang Weixu , Zhou Chongjin , Wang Yanfeng , Zhang Shu , Yang Pinglan , Guo Rui , Chen Wei , Ye Lan , Cui Yiqiang , Ni Ting 
Published: July 2022

Abstract
Mammalian spermatogenesis shows prominent chromatin and transcriptomic switches with delicate morphological and functional alterations in germ cells, but it is unclear how such dynamics are controlled. Here we identify RNA helicase DDX43 as an essential regulator of the chromatin remodeling process during spermiogenesis. Testis-specific Ddx43 knockout mice show male infertility with defective histone-to-protamine replacement and post-meiotic chromatin condensation defects. In addition, the loss of its ATP hydrolysis activity by a missense mutation in the ATPase motif replicates the infertility phenotype in global Ddx43 knockout mice. Single-cell RNA sequencing (scRNA-seq) analyses of germ cells depleted of Ddx43 or expressing the Ddx43 ATPase-dead mutant reveals that DDX43 regulates dynamic RNA regulatory processes that underlie spermatid chromatin remodeling and differentiation. Transcriptomic profiling focusing on early-stage spermatids combined with enhanced crosslinking immunoprecipitation and sequencing (eCLIP-seq) further identify Elfn2 as DDX43-targeted hub gene. Elfn2 knockdown in testis leads to a phenotype similar to Ddx43 knockout or Ddx43 ATPase-dead knock-in. These findings illustrate an essential role for DDX43 in post-meiotic chromatin remodeling and highlight the single-cell-based strategy for a refined dissection of cell-state-specific regulation of germline development.
Synthesis and Application of Fe3O4@Graphene Oxide-Thiobarbituric Acid Based Binary Composite for Selective Removal of Cd(II) from Industrial Wastewater
Authors: Islam Aminul , JAVED HINA 
Published: July 2022

Abstract
In this work, graphene oxide was functionalized with 2-thiobarbituric acid to form a binary composite (Fe3O4@GO-Th) and employed for the selective removal of potentially toxic Cd(II) via batch method. The synthesized composite was well characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Raman spectroscopy. The maximum capacity obtained at pH 5.6, contact time 12 min and successfully eluted by 1 M HCl. The R2 value and six statistical error analysis including residual root-mean-square error, sum of the absolute errors, composite-functional error, average-relative error, etc. defined Freundlich as the best fit isotherm model for Cd(II) adsorption. Moreover, kinetics studies demonstrated pseudo second-order as the best fit model favouring chemisorption behaviour while thermodynamic witnessed the process as endothermic and thermodynamically favourable with spontaneous behaviour. Additionally, Fe3O4@GO-Th showed preferential affinity for Cd(II) in a binary mixture of Cd(II) with other concomitant ions. The practical utility of the synthesized Fe3O4@GO-Th was explored in industrial effluents resulting > 96% removal efficiency for Cd(II) from battery, electroplating, tannery wastewater and tap water.
Transgenic construction and functional miRNA analysis identify the role of miR-7 in prostate cancer suppression
Authors: Xu Bin , Wang Can , Li Wenchao , Hu Qiang , Feng Ninghan , Liu Chun hui , Shi Naipeng , Chen Shuqiu , Chen Ming , Guan Han , You Zonghao 
Published: July 2022

Abstract
Although miR-7 suppresses the initiation and metastasis in cancers, including prostate cancer (PCa), little is known about its efficacy in the treatment of malignancy in vivo, especially in transgenic mouse models. Herein, to demonstrate the safety of in vivo miR-7 gene editing and its suppressive efficacy on p53-negative tumors, for the first time, a miR-7+ transgenic mouse model was constructed and hybridized with the TRAMP mice, whereby p53 was inhibited. miR-7 downregulated the glycolysis of PCa through the HIF pathway, thereby remodeling the acidic tumor macroenvironment, affecting histone lactylation and increasing T cell infiltration. miR-7 suppressed the PCa malignant behavior in LNCaP cells, prostatic primary tumor cells, and mice xenograft models. Moreover, in vivo miR-7 treatment prolonged the survival of mice with PCa and generated synergistic protective effects with PD-1 blockade due to the re-inflamed immune microenvironment. Furthermore, p53 acted as a transcriptional factor upregulating miR-7 expression, and therefore, the sensitization effects of exogenous miR-7 expression on PCa chemotherapeutic efficacy were independent of the p53 status. In summary, our findings highlighted a novel role of miR-7 in PCa. We describe herein, a promising target for the development of miRNA-based therapeutics, especially for PCa patients carrying p53 mutations.
Tailoring polymer electrolyte for high-voltage solid-state Li metal batteries working at ultra-low temperatures
Authors: Li Zhuo , Yu Rui , Weng Suting , Zhang Qinghua , Wang Xuefeng , Guo Xin 
Published: July 2022

Abstract
Stable operation of batteries at low temperatures is critical for applications in cold-climates; however, low-temperature operations are plagued by low ionic conductivity of electrolytes, slow interfacial kinetics and unstable solid–electrolyte interphases (SEIs). We report a polymer electrolyte with an ionic conductivity of 2.2⋅10− 4 S cm− 1 and an ionic transference number of 0.8 at − 20 °C; the electrolyte is synthesized by in-situ polymerizing 1,3,5-trioxane (TXE), 2-2-2-trifluoro-N, N-dimethylacetamide (FDMA), fluoroethylene carbonate (FEC) and lithium difluoro(oxalate)borate (LiDFOB). The polymer electrolyte enables a dual-layered SEI on the Li metal anode and strongly stabilizes the LiNi0.8Co0.1Mn0.1O2 cathode, thus defining fast interfacial charge-transfer at low temperatures. Consequently, the galvanic Li corrosion and the dendritic growth are suppressed, stable Li metal anode and LiNi0.8Co0.1Mn0.1O2 cathode are achieved, and the operation temperatures of polymer-based batteries are decreased to below − 30°C. From − 30°C to room temperature, the Li||LiNi0.8Co0.1Mn0.1O2 battery achieves long cycle lifetime and high capabilities. Even at − 20°C, the Li||LiNi0.8Co0.1Mn0.1O2 battery realizes > 75% (~ 151 mAh g− 1) of its room-temperature capacity, and presents stable cycling over 200 cycles. Outstandingly, the Li||LiNi0.8Co0.1Mn0.1O2 pouch cell successfully powers an electric fan at − 58.3°C.
LncRNA LNCGM1082-mediated NLRC4 activation resists against infection by intracellular bacteria
Authors: Yang Rongcun , Gao Yunhuan , Yang Yazheng , Wei Jianmei , Yue Jianmei , Wang Ya , Zhang Qianjing , Jin Mengli , Wang Rong , Yang Xiaorong , Zhang Junqi , Liu Xinqi , Zhang Yuan 
Published: July 2022

Abstract
The activation of NLRC4 is a major host response against the infection by intracellular bacteria. However, there still remain challenges in understanding the activation upon sensing of diverse stimuli. We here found that lncRNA LNCGM1082 plays a critical role in the activation of NLRC4. LNCGM1082 in macrophages could affect maturation of interleukin (IL)-1β and pyroptotic cell death only after exposed to NLRC4 ligand. Similar to NLRC4-/- mice, LNCGM1082-/- mice were high sensitive to Salmonella typhimurium infection. LNCGM1082 deficiency in mouse or human macrophages had reduced IL-1β maturation and pyroptosis. Mechanistically, LNCGM1082 could induce the combination of PKCδ with NLRC4 in both mice and human. There was absence of binding of NLRC4 with PKCδ in LNCGM1082-/- macrophages. This lncRNA could be induced by Salmonella typhimurium through TLR5 in the macrophages of both mice and human. Thus, our data indicate that LNCGM1082 induced by TLR5 can mediate the binding of PKCδ with NLRC4 to cause the activation of NLRC4.
FFR-SSD: Feature Fusion and Reconstruction Single Shot Detector for Multi-scale Object Detection
Authors: Cheng Xu , Song Chen , Wang Zhixiang , Yu Zitong 
Published: July 2022

Abstract
Object detection is one of the most fundamental tasks toward image content understanding due to their wide applications in the real world. Although numerous algorithms have been proposed, implementing effective and efficient object detection is still very challenging for now, especially for the challenges in restricted situations of multi-size objects and limited semantic information. To solve these challenges, we propose a hierarchical feature fusion and reconstruction method called feature fusion and reconstruction single shot detector (FFR-SSD) for multi-scale object detection, which consists of a multi-branch feature enhancement module, a hierarchical feature weighting strategy and a feature map reconstruction module. First, we present a multi-scale visual attention model, which incorporates the channel-wise and space-wise information into the multi-branch feature enhancement module to improve the feature representation capacities. Second, a hierarchical feature map weighting mechanism is developed to fuse multi-layer feature maps, which contributes to describing the intact objects for the subsequent module. Third, to remove redundant feature information of the fused feature map, we present an effective feature map reconstruction module to encourage the model to focus on pivotal information of the fused feature map. Therefore, the overall contour information of the object is preserved for the shallow enhanced response map and the semantic feature information is enriched for the deep enhanced feature map. Numerous experiments on two public benchmark datasets show that the proposed method achieves significant improvements over the state of the arts.
FAM83H mutation analysis and its expression in rat tooth germs and human dental cells
Authors: Lu Xi , Yu Fang , Ju Yanqin , Yang Jun , Wei Xiaoling , zhao Shouliang , Chen Shuang , Liu Shangfeng 
Published: July 2022

Abstract
Background: Amelogenesis Imperfecta (AI) is a hereditary condition that affect the structure and appearance of dental enamel. It is of three main types, namely hypoplastic, hypomatured, and hypocalcified. Hypocalcified AI, the most severe of all, is generally caused by the mutation of FAM83H (family with sequence similarity 83 member H) gene. Here we aimed to identify the genetic mutations underlying hypocalcified AI in a Chinese family, and to detect the expression of FAM83H in rat tooth germs and human adult dental cells. Methods: A family with hypoplastic AI was recruited and mutational analysis on the candidate gene was performed by Sanger sequencing. Rat tooth germs and human dental cells were distracted and the expression of FAM83H was detected by RNA-seq.Results: Mutational analysis revealed one previously reported mutation (c. 1387C > T) in exon 5 of FAM83H. Clinical study revealed a slightly different phenotype from that previously reported. RNA-sequencing was demonstrated the expression of FAM83H in rat tooth germs and human adult dental cells. Conclusion: The present study identified a reported mutation of FAM83H with a slightly different phenotype, and revealed the FAM83H expression of various development stages of rat tooth germs and human adult dental cells.