bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abnormal functional connectivity of the nucleus accumbens subregions mediates the association between anhedonia and major depressive disorder
Authors: Hu Yanqin , Zhao Chaoqi , Zhao Houfeng , Qiao Juan 
Published: December 2022

Abstract
Background: The nucleus accumbens (Nac) is a crucial brain region in the pathophysiology of MDD patients with anhedonia. However, the relationship between functional imaging characteristics of Nac subregions and anhedonia remains unclear. Thus, this study aimed to investigate the role of functional connectivity of the Nac subregions between MDD and anhedonia.
Methods: We conducted resting-state functional magnetic resonance imaging (fMRI) to measure the resting-state functional connectivity (rsFC) of Nac subregions in 55 MDD patients and 30 healthy controls (HC). A two-sample t-test was performed to obtain the different brain regions with Nac subregions between groups respectively. Then correlation analyses were carried out to investigate the relationships between the aberrant rsFC of Nac subregions and the severity of anhedonia. Furthermore, we constructed a mediation model to explain the role of the aberrant rsFC of Nac subregions between MDD and the severity of anhedonia.
Results: The results show that decreased rsFC of Nac subregions with regions of the prefrontal cortex, insula, lingual, and visual association cortex in MDD patients compared with the HC group. In the MDD group, the rsFC of the right Nac shell-like subregions with middle frontal gyrus (MFG)/superior frontal gyrus (SFG) was correlated with consummatory anhedonia; and the rsFC of the Nac core-like subdivisions with inferior frontal gyrus/insula and lingual/visual association cortex was correlated with anticipatory anhedonia. More importantly, the functional alterations of the Nac subregions mediated the association between anhedonia and depression.
Conclusions: The present findings suggest the functional alteration of the Nac subregions mediates the association between MDD and anhedonia, which provides evidence for the hypothesis that MDD has different neurobiological underpinnings of reward systems from HC.
Effects of probiotic and yeast extract supplementation on oxidative stress, inflammatory response, and growth in weaning Saanen kids
Authors: Kazemi Sajjad , Hajimohammadi Ali , Mirzaei Ahmadreza , Nazifi Saeed 
Published: December 2022

Abstract
To determine the effects of supplementation of probiotics and yeast extract on the metabolic, immune, and oxidative status of the animals during weaning stress, 40 Sannen goat kids were randomly allocated to 4 experimental treatments of probiotic, yeast cell wall extract, probiotic + Yeast extract, and control at 21 days before the expected weaning time. Four blood samples were collected at 21 days pre-weaning, 2 days post-weaning (weaning time), 7 days post-weaning, and 21 post-weaning. Average concentration of Triiodothyronine, thyroxine, total protein, albumin, globulin, blood urea nitrogen, total antioxidant capacity (TAC), serum adenosine deaminase, nitric oxide (NO), Ferritin, glucose, cortisol, triglyceride, NEFA, BHBA, and body-weight was measured. Cortisol concentration (P = 0.02) increased at the weaning time compared to the third sampling time. Treatment tends to (P = 0.10) affect the cortisol concentration in animals treated with probiotics. Concentration of TAC was higher at weaning time compared to the third (P < 0.01) and fourth (P < 0.01) sampling times. Concentration of NO was (P < 0.01) higher in the third sampling time compared to the first sampling time. Treatment affect the concentration of NEFA (P < 0.01) and BHBA (P < 0.01), but sampling time did not affect the NEFA (P = 0.92) and BHBA (P = 0.90) concentration. Increase in TAC and NO at weaning time may indicate an increase in the oxidative processes. Decrease in NEFA and BHBA concentrations may indicate an improvement in animals' metabolic status due to probiotics and yeast extract administration.
Effectiveness of Manual Therapy as an Adjunct to Care as Normal in Female Chronic Migraine Patients. A Randomised Controlled Trial
Authors: Odell James , Branney Jonathan , Hunnisett Adrian 
Published: December 2022

Abstract
Background and purpose
The aim of this study was to explore the effectiveness of manual therapy as an adjunctive treatment to usual care in females severely affected with chronic migraine.
Methods
This was a pragmatic, randomised controlled trial (RCT) in a tertiary headache clinic in the UK. Sixty-four female participants with severe chronic migraine were randomised into two groups: Onabotulinum A (Botox TM) and Botox TM with manual therapy. The Botox TM was given to both groups at the start on a 12-week schedule. The manual therapy was a combination of mobilisation, manipulation and soft tissue release technique and was given 5 times over 12 weeks. The RCT consisted of 3 months intervention with primary outcome measures taken at the end of the study period.
The primary outcome was the between-group difference in change scores using the Headache Impact Test (HIT6). Secondary outcomes were Patient Global Impression of Change (PGIC) and responder rates.
Results
The primary outcome favoured the use of manual therapy as an adjunct to usual tertiary care over usual tertiary care (BotoxTM)alone with a significant difference in between-group HIT6 change scores (p=0.006, d=0.51) The manual therapy group also had significantly more responders based on HIT6 (p=0.006) and PGIC (p= 0.002) outcomes.
Conclusions
This study has found that manual therapy is an effective adjunct to the usual tertiary care (BotoxTM) of chronic migraine in women.
Trial Registration
The trial received a favourable opinion from the UK Health Research Authority (IRAS 228901) and is registered at ClinicalTrials.gov.number NCT03395457. Registered 1st March 2018.
Comprehensive analysis of intercellular communication in thermogenic adipose niche
Authors: Shamsi Farnaz , Zheng Rongbin , Ho Li-Lun , Chen Kaifu , Tseng Yu-Hua 
Published: December 2022

Abstract
Brown adipose tissue (BAT) is responsible for regulating body temperature through adaptive thermogenesis. Cold exposure remodels BAT by increasing the number and function of brown adipocytes, expanding the network of blood vessels and sympathetic nerves, and changing the makeup and function of immune cells. Such synergistic adaptation requires extensive intercellular crosstalk in adipose niche to coordinate their responses. To understand the mechanisms of intercellular communication, we applied the CellChat algorithm to single-cell transcriptomic data of mouse BAT. We constructed an integrative network of ligand-receptor interactome in BAT and identified the major signaling input and output of each cell type. By comparing the ligand-receptor interactions in BAT of mice housed at different temperatures, we found that cold exposure enhances the interactions among the major cell types in BAT, including adipocytes, adipocyte progenitors, lymphatic and vascular endothelial cells, myelinated and non-myelinated Schwann cells, and immune cells. These interactions regulate the remodeling of the extracellular matrix, inflammatory response, angiogenesis, and neurite growth. Together, our integrative analysis of intercellular communications in BAT and their dynamic regulation establishes a holistic understanding of the mechanisms involved in BAT thermogenesis. The resources presented in this study provide a valuable platform for investigations of BAT development and thermogenesis.
Predictive factors of fetal congenital heart block in anti-SS-A antibody-positive pregnant women
Authors: Heshiki Chiaki , Nagai Yutaka , Miyagi Miki , Oku Akira , Kanemura Akari , Kanamine Chihiro , Doi Syoko , Nakasone Tadaharu , Izumi Yuki , Nakano Yuko , Shimabukuro Atsuya , Yamashita Kaoru , Sunagawa Sorahiro , Sakumoto Kaoru , Aoki Yoichi 
Published: December 2022

Abstract
Background
Identifying predictive factors of fetal congenital heart block (CHB) in pregnant women with positive anti-SS-A antibodies is important to manage the cases properly.
Methods
We retrospectively reviewed the medical records of 59 anti-SS-A antibody-positive women undergoing perinatal management at our hospital from January 2011 to November 2021. Univariate and multivariate analyses were performed to investigate predictive factors for the development of fetal CHB.
Results
Of 59 women with positive anti-SS-A antibodies, 9 had fetal CHB. In univariate analysis, fetal CHB was associated with diagnosing connective tissue disease (OR 0.109, p = .009) and positive anti-SS-A antibodies after conception (OR 31.333, p = .0002). In multivariate analysis, diagnosing connective tissue disease was a protective factor (OR 0.049, p = .025), and positive anti-SS-A antibody after conception was a risk factor (OR 41.738, p = .004). However, other maternal clinical characteristics did not influence the development of fetal CHB.
Conclusions
In pregnancies of anti-SS-A antibody-positive women, diagnosing connective tissue disease was an independent protective factor for fetal CHB, and positive anti-SS-A antibody after conception was an independent risk factor.
Clustering pattern and evolution characteristic of microRNAs in grass carp (Ctenopharyngodon Idella)
Authors: Niu Huiqin , Pang Yifan , Xie Lingli , Yu Qiaozhen , Shen Yubang , Li Jiale , Xu Xiaoyan 
Published: December 2022

Abstract
Background
A considerable fraction of miRNAs are highly conserved, and certain miRNAs correspond to genomic clusters. The clustering of microRNAs can be advantageous, possibly by allowing coordinated expression. However, little is known about the evolutionary forces responsible for the loss and acquisition of microRNA and microRNA clusters.
Results
The results demonstrated that several novel miRNAs arose throughout grass carp evolution. Duplication and de novo production were critical strategies for miRNA cluster formation. Duplicates accounted for a smaller fraction of the expansion in the grass carp miRNA than de novo creation. Clustered miRNAs are more conserved and change slower, whereas unique miRNAs usually have high evolution rates and low expression levels. The expression level of miRNA expression in clusters is strongly correlated.
Conclusions
This study examines the genomic distribution, evolutionary background, and expression regulation of grass carp microRNAs. Our findings provide novel insights into the genesis and development of microRNA clusters in teleost.
Effect of Preoperative Pupil Offset on Corneal Higher-Order Aberrations after Femtosecond Laser-Assisted In Situ Keratomileusis
Authors: Liu Zhanglin , Zhao Yang , Sun Shengshu , Wu Yuan , Wang Guiqin , Zhao Shaozhen , Huang Yue 
Published: December 2022

Abstract
Background
This study aimed to investigate the relationship between multiple higher-order aberrations (HOAs) subgroups and pupil offset, as well as to analyze the factors affecting postoperative corneal HOAs in patients with different degrees of refractive errors.
Methods
We enrolled 160 patients (316 eyes) aged ≥ 18 years who had undergone femtosecond laser-assisted in situ keratomileusis (FS-LASIK) treatment. Based on the relationship between the preoperative pupil offset and the postoperative ΔHOAs, all patients were divided into two groups: group I (pupil offset ≤ 0.20 mm) and group II (pupil offset > 0.20 mm). All of the eyes had low to high myopia with or without astigmatism (manifest refraction spherical equivalent (MRSE) < -10.00 D). Uncorrected distance visual acuity, corrected distance visual acuity, MRSE, pupil offset, central corneal thickness, corneal HOAs, vertical coma (Z3 − 1), horizontal coma (Z31), spherical aberration (Z40), trefoil 0° (Z33), and trefoil 30° (Z3 − 3) over a 6 mm diameter central corneal zone diameter were evaluated preoperatively and at 1 and 3 months postoperatively.
Results
Our result revealed significant differences in postoperative corneal total root mean square (RMS) HOAs, RMS vertical coma, RMS horizontal coma, RMS spherical aberration, and RMS trefoil 30° between group I and group Ⅱ. ΔMRSE was found to be an effective factor for ΔRMS HOAs (R2 = 0.383), ΔRMS horizontal coma (R2 = 0.205), and ΔRMS spherical aberration (R2 = 0.397). In group II, multiple linear regression analysis revealed a significant correlation between preoperative pupillary offset and Δtotal RMS HOAs (R2 = 0.461), ΔRMS horizontal coma (R2 = 0.040), and ΔRMS trefoil 30°(R2 = 0.089). The ΔRMS vertical coma effect factor is the Y-component, and the factor influencing ΔRMS spherical aberration was ΔMRSE (R2 = 0.256).
Conclusion
A small pupil offset was associated with a lower induction of postoperative corneal HOAs. Efforts to optimize centration are critical for improving surgical outcomes in patients with FS-LASIK.
Bloom succession and nitrogen dynamics during snowmelt in a mid-order montane river
Authors: Valett Maurice , de Lima Rafael Feijó , Peipoch Marc , Engstrom Royce 
Published: December 2022

Abstract
The Upper Clark Fork River, Montana, a system with a recent history of remediation and restoration from historic mine contamination, was studied to assess succession of riverine periphyton and biogeochemical characteristics following snowmelt runoff and into summer baseflow conditions. Using an historical twenty-year database and three recent field seasons, algal abundance and growth forms were tracked along with physicochemical characteristics, nitrogen (N) and phosphorous (P) dynamics, and rates of Nfixation at three sites. During snowmelt recession, major blooms of the filamentous green algae Cladophora occurred across all sites, commensurate with declines in dissolved inorganic N. Atomic N:P ratios suggest successional transitions from P- to N-limitation associated with mid-season senescence of Cladophora and development of a secondary bloom of N-fixing algae, dominated by Nostoc cf.pruniforme. Rates of N-fixation increased upon Cladophora senescence to a maximal value of 5.80 mg N/m2/h before decreasing again to background levels at the end of the growing season. Results suggest successional transitions from green algal blooms driven by allochthonous N supply to stages dominated by cyanobacteria and autochthonous N production. Results have implications for continuing river restoration as mediation of anthropogenic N inputs may enhance successional transition to secondary blooms dominated by cyanobacterial forms.
Identification and Prognostic Analysis of Ferroptosis-related Genes to Predict The Progression of Lung Squamous Cell Carcinoma
Authors: Guo Di , Feng Yonghai , Liu Peijie , Yang Shanshan , Zhao Wenfei , Li Hongyun 
Published: December 2022

AbstractBackground:Ferroptosis, an iron-dependent form of regulated cell death driven by excessive lipid peroxidation, has been implicated in the development and therapeutic responses of cancer. However, the role of ferroptosis-related gene profiles in LSCCremains largely unknown. This study aims to identify the prognostic roles of ferroptosis-related genes in LSCC.Methods:We analyzed sequencing data from the Cancer Genome Atlas and identified ferroptosis-related gene expressionbetween tumors and para‐tumors. We also assessed the prognostic role of these genes using Kaplan–Meier analysis and univariable and multivariable Cox proportional hazards regression model analyses. Immunological correlation, tumor stemness, and drug sensitivity and the transcriptional differences of HSPA5 were also analyzed in LSCC. Then, we investigated the expression of HSPA5 usingimmunohistochemistry in 100 patients with metastatic LSCC and assessed the clinical significance of these markers with different risk factors.Results:Of the 22 ferroptosis-related genes, the expression of HSPA5, HSPB1, GPX4, FANCD2, CISD1, FDFT1, NFE2L2, SLC1A5, RPL8, NCOA4, TFRC, and SLC7A11 was significantly increased in LSCC compared to adjacent tissues. However, only high expression of HSPA5 can predict progression-free survival (PFS) and disease-free survival time (DFS) in LSCC. Although HSPA5 was also significantly elevated in patients with lung adenocarcinoma, HSPA5 expression did not predict the prognosis of lung adenocarcinoma patients. Notably, higher expression of HSPA5 showed higher responses to chemotherapy but not immunotherapy. Interestingly, HSPA5 expression positively correlated with ferroptosis, cellular responses to hypoxia, tumor proliferation signature, G2M checkpoint, MYC targets, and TGFB. IHC analysis also confirm the increased expression of HSPA5 in metastatic LSCC patients in our cohort. And higher expression of HSPA5 in metastatic LSCC patients demonstrated shorter PFS and overall survival time (OS).Conclusions:This study shows that ferroptosis-related gene HSPA5 expression is a negative prognostic marker for LSCC.
Non-Operative Treatment for Simple Acute Appendicitis (NOTA) in Children during COVID -19 Era: New Lessons from the Pandemic
Authors: Alsaggaf Ameen , Owiwi Yazeed , Shalaby Mohamed , Ghallab Alaa , Zidan Mazen , Alawi Ahmed , Bustangi Nasir , Awad Mohammed , Bana Abdulelah , Zeair Saad Al , Afandi Ahmed , Basyouni Ahmed , Nasser Ibrahim Al , Raml Enas , Raboei Enaam 
Published: December 2022

Abstract
Background:

Coronavirus (COVID-19) is the newest pandemic disease. The surgical management of acute appendicitis was the gold standard, but new studies suggest the safety of antibiotics treatment alone. NOTA avoid surgery, risks of general anesthesia; long hospital stay and risk of corona virus exposure. We aim to study the safety, cost effectiveness and outcome of NOTA during Covid-19 pandemic and compare it to Single incision pediatric endo-surgery appendectomy (SIPESA)

Methods

A prospective cohort study for NOTA of patients from 6–12 years old in Covid-19 pandemic period from 1st April 2020 to 30th April 2021. Patients divided in 2 groups: Group S managed by SIPESA; group N managed by NOTA. Family education and assurance with detailed explanation was done for early detection of any complication and we continue monitoring of the patients until complete recovery.

Results

sixty patients were managed. Group S had 24 cases (40%), mean age 9.3 years. Group N had 36 cases (60%), mean age 9.1 years. six cases (17%) in group N were converted to surgical management in first 6 month of study. Mean length of hospital stay dropped from 72 hours to 18 hours. The mean cost dropped from 2736$/day to 400$/day. Mean psychological stress for the children improved from 4.4 in April to 2 in September. Mean follow up was 3.5 months.

Conclusions

NOTA is safe and cost-effective. we recommend it especially during the COVID-19 pandemic.
Level of evidence: Cost Effectiveness Study: Level II
Alterations of Choroidal and Retinal Microvasculature in Heart Failure Patients with Reduced Ejection Fraction
Authors: Khalilipur Ehsan , Mahdizad Zahra , Molazadeh Negin , Faghihi Hooshang , Naderi Nasim , Bahar Mohammadreza Mehrabi , Firouzi Ata , Sadeghipour Parham , Maleki Majid , Shahgoli Sahel Soltani , Pour Elias Khalili , Riazi-Esfahani Hamid 
Published: December 2022

Abstract
This cross-sectional study designed to assess alterations of choroidal and retinal microvasculature in patients with Heart Failure with Reduced Ejection Fraction (HFrEF) and compare them with a normal age-matched population. Fifty-two eyes of 26 patients with HFrEF (left ventricular ejection fraction [LVEF] < 40%) and 64 eyes of 32 healthy individuals considered as the patient and the control groups, respectively. The whole image, foveal, parafoveal, and perifoveal age-adjusted mean superficial and deep capillary plexus VD showed statistically significant differences between the HFrEF group and the normal controls (P = 0.007, P = 0.000, P = 0.006, and P = 0.026, respectively). The patients with HFrEF had a significantly lower subfoveal choroidal thickness (SFCT) than the normal subjects (264 ± 82 vs 313 ± 72, respectively; P = 0.009), and the difference was still statistically significant after age adjustment (P = 0.004). Although choroidal vascularity index (CVI) was lower in the HFrEF group than in the control group, the difference was not statistically significant before and after age adjustment (73.45 ± 6.67 vs 75.77 ± 5.92, respectively; P = 0.118 and P = 0.098, respectively). In conclusion, in patients with HFrEF, we observed a reduction in retinal VD in both the superficial and deep capillary plexuses, as well as SFCT, but no significant change in CVI.
A case of severe infection of metacercariae from the liver of garfish Xenentodon cancila (Beloniformes: Belonidae): morphological and molecular evidence
Authors: Chaudhary Anshu , Singh Komal , Sharma Bindu , Singh Hridaya Shanker 
Published: December 2022

Abstract
Using only the morphological traits to describe trematode parasites are a poor indicator of their identification and validation. The current work uses molecular data to describe diplostomoid metacercariae supplemented with morphology found in freshwater garfish, Xenentodon cancila Ham., 1822 collected from River Ganga, of district Bijnour, Meerut region (Uttar Pradesh), India. The metacercariae were identified as Posthodiplostomum pandei (= Neascus pandei) collected from liver shows a high mass of cysts embedded and surrounded by partially damaged liver tissue. Partial DNA sequences of the 18S, internal transcribed spacers (ITS1-5.8S-ITS2) and 28S of nuclear ribosomal DNA were generated and compared with available sequences on the Genbank database. In the phylogenetic analysis of partial sequences from 18S, ITS cluster (ITS1-5.8S-ITS2) and 28S rDNA, P. pandei from India fell within the superfamily Diplostomoidea with other members of Posthodiplostomum also confirms its distinct status and was found close to other Indian species. In Indian region, along with morphology, many species described under Neascus-type metacercariae are awaiting for their validation to be supplemented with molecular data. Furthermore, the validity of few species of genus Posthodiplostomum is also discussed to synonymized with P. pandei in the present study.
Volterra Operator-Based Fixed-Time Adaptive Parameter Estimation for DC-DC Buck Converters without Current Sensors
Authors: Shi Shang , Lu Jiazhou , Min Huifang , Hu Yinlong 
Published: December 2022

Abstract
This paper investigates the problem of parameter estimation for DC-DC buck converter without current sensors. For the circuit, all the parameters of capacitance, inductance, resistance and input voltage are unknown. A novel Volterra operator-based fixed-time adaptive algorithm is proposed by using only the output voltage and control input signals. By selecting proper kernel function for the Volterra integral operator, the influence of the system initial values can be eliminated and the calculation of the derivative of the system output can also be avoided. Strict analysis shows that the proposed estimation algorithm can ensure the estimation errors converge to zero in a fixed time independent of the initial error values. Finally, simulation results with different initial values verify the advantages of the proposed algorithm.