bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine
Authors: Chen Yuanting , Huang Huacong , Li Gaoxing , Yu Jianyu , Fang Fuchun , Qiu Wei 
Published: January 2022

AbstractStem cells transplantation is the main method of tissue engineering regeneration treatment, the viability and therapeutic efficiency are limited. Scaffold materials also play an important role in tissue engineering, whereas there are still many limitations, such as rejection and toxic side effects caused by scaffold materials. Cell sheet engineering is a scaffold-free tissue technology, which avoids the side effects of traditional scaffolds and maximizes the function of stem cells. It is increasingly being used in the field of tissue regenerative medicine. Dental-derived mesenchymal stem cells (DMSCs) are multipotent cells that exist in various dental tissues and can be used in stem cell-based therapy, which is impactful in regenerative medicine. Emerging evidences show that cell sheets derived from DMSCs have better effects in the field of regenerative medicine applications. Extracellular matrix (ECM) is the main component of cell sheets, which is a dynamic repository of signalling biological molecules and has a variety of biological functions and may play an important role in the application of cell sheets. In this review, we summarized the application status, mechanisms that sheets and ECM may play and future prospect of DMSC sheets on regeneration medicine.
Matrix vesicles from dental follicle cells improve alveolar bone regeneration via activation of the PLC/PKC/MAPK pathway
Authors: Yi Genzheng , Zhang Siyuan , Ma Yue , Yang Xueting , Huo Fangjun , Chen Yan , Yang Bo , Tian Weidong 
Published: January 2022

AbstractBackgroundThe regeneration of bone loss that occurs after periodontal diseases is a significant challenge in clinical dentistry. Extracellular vesicles (EVs)-based cell-free regenerative therapies represent a promising alternative for traditional treatments. Developmental biology suggests matrix vesicles (MVs), a subtype of EVs, contain mineralizing-related biomolecules and play an important role in osteogenesis. Thus, we explore the therapeutic benefits and expect to find an optimized strategy for MV application.MethodsHealthy human dental follicle cells (DFCs) were cultured with the osteogenic medium to generate MVs. Media MVs (MMVs) were isolated from culture supernatant, and collagenase-released MVs (CRMVs) were acquired from collagenase-digested cell suspension. We compared the biological features of the two MVs and investigated their induction of cell proliferation, migration, mineralization, and the modulation of osteogenic genes expression. Furthermore, we investigated the long-term regenerative capacity of MMVs and CRMVs in an alveolar bone defect rat model.ResultsWe found that both DFC-derived MMVs and CRMVs effectively improved the proliferation, migration, and osteogenic differentiation of DFCs. Notably, CRMVs showed better bone regeneration capabilities. Compared to MMVs, CRMVs-induced DFCs exhibited increased synthesis of osteogenic marker proteins including ALP, OCN, OPN, and MMP-2. In the treatment of murine alveolar bone defects, CRMV-loaded collagen scaffold brought more significant therapeutic outcomes with less unhealing areas and more mature bone tissues in comparison with MMVs and acquired the effects resembling DFCs-based treatment. Furthermore, the western blotting results demonstrated the activation of the PLC/PKC/MAPK pathway in CRMVs-induced DFCs, while this cascade was inhibited by MMVs.ConclusionsIn summary, our findings revealed a novel cell-free regenerative therapy for repairing alveolar bone defects by specific MV subtypes and suggest that PLC/PKC/MAPK pathways contribute to MVs-mediated alveolar bone regeneration.
LINC01119 negatively regulates osteogenic differentiation of mesenchymal stem cells via the Wnt pathway by targeting FZD4
Authors: Gao Hongwei , Dong Hui , Zheng Jiachun , Jiang Xufeng , Gong Mingzhi , Hu Le , He Jinshan , Wang Yongxiang 
Published: January 2022

Abstract
Background
Mesenchymal stem cells (MSCs) can differentiate into diverse cell types under specific conditions. Dysfunction in the osteogenic differentiation of MSCs can result in bone metabolism-related diseases, including osteoporosis. Accumulating evidence has revealed that long non-coding RNA (lncRNAs) play critical regulatory roles during MSC differentiation.

Methods
In the present study, we identified an evolutionarily conserved lncRNA expressed during the osteogenic differentiation of MSCs, which we termed LINC01119. We first identified LINC01119 as a negative regulator of the osteogenic differentiation of MSCs.

Results
LINC01119 knockdown markedly induced calcium deposition in bone marrow MSCs and promoted the osteogenic differentiation of MSCs. More importantly, we demonstrated the underlying molecular basis through which LINC01119 regulates osteogenesis via the Wnt pathway by targeting FZD4. Furthermore, we observed that transcription factor EBF3 could directly bind the promoter site of LINC01119.

Conclusions
We first explored the molecular regulatory mechanism of LINC01119 during the osteogenic differentiation of MSCs and revealed that LINC01119 negatively regulates osteogenesis through the Wnt pathway by targeting FZD4.
Factors predisposing women and children to indoor air pollution in rural villages, Western Kenya
Authors: Dida Gabriel O. , Lutta Patrick O. , Abuom Paul O. , Mestrovic Tomislav , Anyona Douglas N. 
Published: January 2022

Abstract
Background
Indoor air pollution (IAP) remains a major global public health hazard more so in developing countries where use of fossil fuels is still very common. However, despite the popularity of kerosene and fuelwood as energy sources among many households in the Sub-Saharan Africa, little is known about their health effects and the predisposing factors particularly on those with direct exposure. This study sought to relate indoor air pollution exposure to self-reported prevalence of respiratory outcomes including (sputum production, congestion, breathing difficulties, eye problems, fatigue, and headaches and wheezing) among women and children of Trans Nzoia County, in the rural villages of western Kenya.

Methods
In this cross-sectional survey, simple random technique was used to select 251 households from 14 villages. Households were the sampling units, while the woman of the household with/or in custody of a child aged less than 5 years old were the unit of analysis. A total of 251 women with/or in custody of a child aged less than 5 years old took part in the study. A structured questionnaire was used to collect information on cause and effects of IAP among women and children. Data was analyzed descriptively and inferentially. We used Poisson generalized linear models with IAP symptoms and indoor cooking as dependent variables and household profiles and other socio-demographics as independent variables to identify the factors that affect health outcome.

Results
Mean age of respondents was 36.49 years, (95% CI [35.5, 37.5]). Most (64.5%) houses were semi-permanent, with 58.6% having an average kitchen size (5.6 m2). Wood and kerosene were the most preferred fuel types for cooking (96.8%) and lighting (97.4%), respectively. Smoke from the wood was identified as the dominant (96.8%) source of IAP. Most women (92.0%) and children (95.4%) had coughs of varying intensities during the year, while 31.5% of the women reported wheezing. About 98% of them experienced fatigued and headaches. Use of wood fuel was associated with increased coughing (p = 0.03), phlegm (p = 0.02), wheezing (p = 0.04), eye problems (p = 0.03) and headaches (p = 0.01) among women and children in the previous 24 h. Education level, ventilation, main fuel source used in 24 h, indoor cooking and house type were significantly associated with IAP health effects (p ≤ 0.05).

Conclusions
Supporting the impoverished households and increasing their level of awareness on health-effects of IAP occasioned by use of biomass fuel while cooking indoors may be the first step in implementing a programme aimed at reducing exposure among rural households in Trans Nzoia County, in rural parts of Western Kenya.
Faecal microbiota transplantation reduces amounts of antibiotic resistance genes in patients with multidrug-resistant organisms
Authors: Hyun JongHoon , Lee Sang Kil , Cheon Jae Hee , Yong Dong Eun , Koh Hong , Kang Yun Koo , Kim Moo Hyun , Sohn Yujin , Cho Yunsuk , Baek Yae Jee , Kim Jung Ho , Ahn Jin Young , Jeong Su Jin , Yeom Joon Sup , Choi Jun Yong 
Published: January 2022

Abstract
Background
Multidrug-resistant organisms (MDROs) such as vancomycin-resistant enterococci (VRE) and carbapenemase-producing Enterobacteriaceae (CPE) are associated with prolonged hospitalisation, increased medical costs, and severe infections. Faecal microbiota transplantation (FMT) has emerged as an important strategy for decolonisation. This study aimed to evaluate the genetic response of MDROs to FMT.

Methods
A single-centre prospective study was conducted on patients infected with VRE, CPE, or VRE/CPE who underwent FMT between May 2018 and April 2019. Genetic response was assessed as the change in the expression of the resistance genes VanA, blaKPC, blaNDM, and blaOXA on days 1, 7, 14, and 28 by real-time reverse-transcription polymerase chain reaction.

Results
Twenty-nine patients received FMT, of which 26 (59.3%) were infected with VRE, 5 (11.1%) with CPE, and 8 (29.6%) with VRE/CPE. The mean duration of MDRO carriage before FMT was 71 days. Seventeen patients (63.0%) used antibiotics within a week of FMT. In a culture-dependent method, the expression of VanA and overall genes significantly decreased (p = 0.011 and p = 0.003 respectively). In a culture-independent method, VanA, blaNDM, and overall gene expression significantly decreased over time after FMT (p = 0.047, p = 0.048, p = 0.002, respectively). Similar results were confirmed following comparison between each time point in both the culture-dependent and -independent methods. Regression analysis did not reveal important factors underlying the genetic response after FMT. No adverse events were observed.

Conclusion
FMT in patients infected with MDROs downregulates the expression of resistance genes, especially VanA, and facilitates MDRO decolonisation.
A framework for research into continental ancestry groups of the UK Biobank
Authors: Constantinescu Andrei-Emil , Mitchell Ruth E. , Zheng Jie , Bull Caroline J. , Timpson Nicholas J. , Amulic Borko , Vincent Emma E. , Hughes David A. 
Published: January 2022

Abstract
Background
The UK Biobank is a large prospective cohort, based in the UK, that has deep phenotypic and genomic data on roughly a half a million individuals. Included in this resource are data on approximately 78,000 individuals with “non-white British ancestry.” While most epidemiology studies have focused predominantly on populations of European ancestry, there is an opportunity to contribute to the study of health and disease for a broader segment of the population by making use of the UK Biobank’s “non-white British ancestry” samples. Here, we present an empirical description of the continental ancestry and population structure among the individuals in this UK Biobank subset.

Results
Reference populations from the 1000 Genomes Project for Africa, Europe, East Asia, and South Asia were used to estimate ancestry for each individual. Those with at least 80% ancestry in one of these four continental ancestry groups were taken forward (N = 62,484). Principal component and K-means clustering analyses were used to identify and characterize population structure within each ancestry group. Of the approximately 78,000 individuals in the UK Biobank that are of “non-white British” ancestry, 50,685, 6653, 2782, and 2364 individuals were associated to the European, African, South Asian, and East Asian continental ancestry groups, respectively. Each continental ancestry group exhibits prominent population structure that is consistent with self-reported country of birth data and geography.

Conclusions
Methods outlined here provide an avenue to leverage UK Biobank’s deeply phenotyped data allowing researchers to maximize its potential in the study of health and disease in individuals of non-white British ancestry.
Predicting temperature and precipitation during the flood season based on teleconnection
Authors: Jung Jaewon , Kim Hung Soo 
Published: January 2022

AbstractIn recent years, the damages resulting from abnormal hydrometeorological climate have substantially increased over the world due to the climate variability and change. Especially, the flood damage has been severely occurred during the flood season almost every year in Korea. For an example, we had the localized heavy rainfalls for 54 days in flood season of 2020 and had huge property damage and loss of life. Therefore, the study needs to be conducted to improve the predictive power of seasonal time-scale forecasts spanning one to several months for the damage reduction and prevention. In this regard, this study aims to provide a priori predictions (several months ahead) of the climate variable at target sites with a statistical method based on teleconnection with global climatic conditions. Herein, the paradigm of the temperature and precipitation prediction in the Geum river basin in Korea is presented. The purposes of the study are also (1) to analyse the characteristics of summer temperatures and precipitation according to the occurrence of El Niño/La Niña and (2) to suggest a seasonal prediction model that can consider the effects of the occurrence of El Niño and La Niña during the flood season. The model is constructed by classifying the data period into El Niño, La Niña, and neutral status. Then we have shown that the prediction model improves the predictive power for the predictions of climate variables such as temperature and precipitation at mid-latitude stations which Korea is located. Therefore, this study demonstrates the possibility of improving the predictive power for forecasting temperature and precipitation by the prediction model considering climate variability.
Evolution of the magma plumbing system of Miyakejima volcano with periodic recharge of basaltic magmas
Authors: Geshi Nobuo , Oikawa Teruki , Weller Derek J. , Conway Chris E. 
Published: January 2022

AbstractDefining the variations in petrological characteristics of erupted magmas within a high-resolution chronostratigraphy provides a necessary framework for monitoring the long-term activity and eruption potential of an active volcano. Here, we investigate the evolution of the magmatic system of Miyakejima volcano, Japan, between the last two caldera-forming eruptions, at ~ 2.3 ka and AD 2000, based on new stratigraphic constraints, radiocarbon ages, and whole-rock geochemical data. The activity of Miyakejima during this interval can be divided into three magmatic periods based on cyclic whole-rock compositional trends. Period 1 spans the interval between ~ 2.3 ka and the 7th century, from the Hatchodaira eruption with caldera collapse to immediately before the Suoana–Kazahaya eruption. Period 2 spans the time period between the seventh century and the fourteenth century, from the Suoana–Kazahaya to the Sonei–bokujyo eruptions. Period 3 covers the period from the two major flank eruptions that occurred in the sixteenth century to the end of the twentieth century until the last caldera-collapse event in AD 2000. The eruption rate decreased from 0.5 km3 per 1000 years in Period 1 to ~ 0.2 km3 per 1000 years in Period 2 and 3. Recharge of primitive basaltic magmas into shallower crustal systems triggered extensive basaltic fissure eruptions at the beginning of each period. Progressively increasing whole-rock SiO2 contents of the hybrid magmas in subsequent eruptions indicates continuous fractional crystallization in small shallow magma chambers which formed at the start of each magmatic period. Intermittent injections of basaltic magma into shallow magma chambers induced magma mixing that caused eruption of hybrid basaltic andesite in each period. We suggest that some basaltic magmas formed isolated magma reservoirs at shallow depth, in which rapid fractionation was able to occur. Rupturing of these isolated magma storage regions filled with gas-rich evolved magma can lead to violent ejection of andesitic magmas, such as for the Suoana–Kazahaya eruption in the seventh century. Our results suggest two main scenarios of eruption for the basaltic magma system at Miyakejima and similar mafic volcanoes in the northern Izu–Bonin arc; (1) eruption of voluminous basaltic lavas after the recharge of primitive basaltic magmas into the shallow magmatic system, and (2) explosive fissure eruption by rupturing of isolated magma bodies filled with gas-rich evolved magmas.
Graphical Abstract
Unusual giant multilocular thymic cyst with mature teratoma including a carcinoid component in the mediastinum
Authors: Manabe Takehiko , Kajiyama Kenta , Iwanami Takashi , Hanagiri Takeshi , Sako Tatsuhiko 
Published: January 2022

Abstract
Background
Teratoma is the second most common mediastinal neoplasm, but malignant transformation in mature teratomas is uncommon, and cases of carcinoid tumor with teratoma are described in only a few studies. In addition, multilocular thymic cyst associated with mature mediastinal teratoma is also a rare entity. There have been no reports of case with the coexistence of these three pathological lesions.

Case presentation
The patient was a 24-year-old man who was referred to our hospital due to a 2-day history of left shoulder pain, a feeling of severe chest tightness and high fever. Pre-operative computed tomography (CT) showed a large, fluid-filled and well-demarcated multilocular cyst in the anterior to superior mediastinum measuring up to 12 cm in size. Contrast-enhanced CT also revealed that the tumor contained a solid component with slight contrast enhancement and spotty wall-thickening septation. Therefore, cystic thymoma, thymic cyst, cystic teratoma, or germ cell tumor with an inflammatory reaction were considered as differential diagnoses. The patient underwent tumor extirpation under median sternotomy. The pathological diagnosis was multilocular thymic cyst with mature teratoma including carcinoid tumor (Grade 2) in the mediastinum.

Conclusions
The relationship between thymic cyst, teratoma and carcinoid tumor is unclear at present; therefore, further research is needed to clarify the relationship between these entities. In this report, we present a case of multilocular thymic cyst with mature teratoma including a carcinoid component in the mediastinum that was detected by complete surgical resection.
The effect of initiation of urate-lowering treatment during a gout flare on the current episode: a meta-analysis of randomized controlled trials
Authors: Jia Ertao , Yao Xiaoling , Geng Hongling , Zhong Li , Xie Jingjing , Xiao Yuya , Jiang Yubao , Qiu Xia , Xiao Min , Zhang Yanying , Tang Dabin , Wei Jiaxin , Zhang Jianyong 
Published: January 2022

Abstract
Objective
The objective was to evaluate whether initiation of urate-lowering treatment (ULT) during an acute gout flare prolonged the current episode.

Methods
A comprehensive search of MEDLINE and Web of Science databases was conducted from their inception to 15 March 2021. Five randomized controlled trials (RCTs) with 381 patients met the inclusion criteria. Standardized mean difference (SMD), odds ratio (OR), and 95% confidence interval (CI) were used for estimating the clinical efficacy of ULT in acute gout.

Results
There was no statistical difference in days to resolution (intent-to-treat analysis) (SMD, 0.68; 95% CI − 0.42 to 1.78; I2, 49%; p = 0.22), the pain visual analogue score (VAS) by day 10 (SMD, − 0.07; 95% CI − 0.30 to 0.16; I2, 0%; p = 0.53), C-reactive protein (CRP) from day 7 to 10 (SMD, − 1.14; 95% CI − 5.63 to 3.36; I2, 55%; p = 0.62), erythrocyte sedimentation rate (ESR) from day 7 to 10 (SMD, − 2.51; 95% CI − 5.46 to 0.45; I2, 0%; p = 0.10) and the recurrence of gout flares within 28–30 days (OR 0.78; 95% CI 0.29 to 2.09; I2, 0%; p = 0.62).

Conclusion
Initiation of ULT during an acute gout flare did not prolong the duration of the flare. However, larger sample size studies are needed to confirm this finding.
Trial registration number PROSPERO (CRD42021234581).
The nanocomposites designs of phytomolecules from medicinal and aromatic plants: promising anticancer-antiviral applications
Authors: Kaplan Ayse 
Published: January 2022

AbstractBackgroundNowadays, researchers are moving toward a herbal approach to cancer treatment because of the harmful effects of synthetic anti-tumor drugs. The evaluation of active compounds with plant origin may help in the remedy of human illnesses in the future. These active compounds have direct or indirect curative efficacies on difficult to cure diseases such as cancer. Investigation of nanoforms of these active compounds is one of the curious topics of the scientific community.Main bodySaffron and its components obtained fromCrocus sativa, essential oils obtained from lavender,Syzygium aromaticumcalled cloves andBeta vulgarisare known for their anticancer effects. Nano-drugs are designed to increase the anticancer activity of plant-derived drugs. Herbal extracts operate very great in the production of nanoparticles. The aim is to ensure that only the nano-drug is delivered to the tumor site. Furthermore, nanoparticles have hazardous effects when analyzed at elevated doses, but this issue can be doped together with plant extracts.Short conclusionsThe nanocomposites (graphene oxide, solid lipid nano and nanoemulsion) of phytomolecules obtained from saffron, clove, lavender and red beet may be effective in minimizing these toxic effects. In the near future, detecting the anticancer molecular mechanisms of these naturally derived compounds and nanocomposites could contribute to further cancer research. Apart from these, these compounds and its nanocomposites could have antiviral effects against today's threat covid-19 virus. Consequently, more promising anticancer and antiviral agents would be discovered.Graphical abstract
Relation between pulmonary function tests and ultrasonographic changes of asymptomatic anterior chest wall joints in rheumatoid arthritis patients
Authors: Mortada Mohammed A. , Abdelrahman Fatma Ibrahim , Abdul-Sattar Amal , Mansour Waleed 
Published: January 2022

Abstract
Background
Ultrasonography can detect different changes in anterior chest wall (ACW) joints in patients with rheumatoid arthritis (RA) even before being clinically manifested. Airways, pleura, lung parenchyma, and vascular compartment all may be attacked by RA. This study was aiming at detecting the relation between ultrasonographic changes of asymptomatic ACW joints and pulmonary function tests (PFTs) in patients with RA.

Results
US detected subclinical changes of ACW joints in (74.2%) of RA patients with significant difference between total US changes in RA (74.2%) and control (21.2%) (p < 0.001). MSJ ankylosing and erosions were highly associated with limited chest expansion in RA group (P <0.001). Restrictive PFTs were associated with SCJ synovitis (p < 0.05), SCJ PD activity (p < 0.05), SCJ erosions (0.02), and highly associated with MSJ ankylosing and erosions (p < 0.001).

Conclusions
This study demonstrated that ultrasonographic subclinical changes in ACW joints are associated with restrictive pattern by spirometry and limited chest expansion in RA patients.

Trial registration
ClinicalTrials.gov Identifier: NCT05119491. Registered 15 November 2021—retrospectively registered.