bluuebunny/crossref_metadata_2025_split huggingface dataset:
NLRP3 licenses NLRP11 for inflammasome activation in human macrophages
Authors: Gangopadhyay Anu , Devi Savita , Tenguria Shivendra , Carriere Jessica , Nguyen Huyen , Jäger Elisabeth , Khatri Hemisha , Chu Lan H. , Ratsimandresy Rojo A. , Dorfleutner Andrea , Stehlik Christian 
Published: May 2022

AbstractIntracellular sensing of stress and danger signals initiates inflammatory innate immune responses by triggering inflammasome assembly, caspase-1 activation and pyroptotic cell death as well as the release of interleukin 1β (IL-1β), IL-18 and danger signals. NLRP3 broadly senses infectious patterns and sterile danger signals, resulting in the tightly coordinated and regulated assembly of the NLRP3 inflammasome, but the precise mechanisms are incompletely understood. Here, we identified NLRP11 as an essential component of the NLRP3 inflammasome in human macrophages. NLRP11 interacted with NLRP3 and ASC, and deletion of NLRP11 specifically prevented NLRP3 inflammasome activation by preventing inflammasome assembly, NLRP3 and ASC polymerization, caspase-1 activation, pyroptosis and cytokine release but did not affect other inflammasomes. Restored expression of NLRP11, but not NLRP11 lacking the PYRIN domain (PYD), restored inflammasome activation. NLRP11 was also necessary for inflammasome responses driven by NLRP3 mutations that cause cryopyrin-associated periodic syndrome (CAPS). Because NLRP11 is not expressed in mice, our observations emphasize the specific complexity of inflammasome regulation in humans.
Proteomic and transcriptomic profiles of human urothelial cancer cells with histone deacetylase 5 overexpression
Authors: Jaguva Vasudevan Ananda Ayyappan , Hoffmann Michèle J. , Poschmann Gereon , Petzsch Patrick , Wiek Constanze , Stühler Kai , Köhrer Karl , Schulz Wolfgang A. , Niegisch Günter 
Published: May 2022

AbstractUrothelial carcinoma (UC) of the urinary bladder is a prevalent cancer worldwide. Because histone deacetylases (HDACs) are important factors in cancer, targeting these epigenetic regulators is considered an attractive strategy to develop novel anticancer drugs. Whereas HDAC1 and HDAC2 promote UC, HDAC5 is often downregulated and only weakly expressed in UC cell lines, suggesting a tumor-suppressive function. We studied the effect of stable lentiviral-mediated HDAC5 overexpression in four UC cell lines with different phenotypes (RT112, VM-Cub-1, SW1710, and UM-UC-3, each with vector controls). In particular, comprehensive proteomics and RNA-seq transcriptomics analyses were performed on the four cell line pairs, which are described here. For comparison, the immortalized benign urothelial cell line HBLAK was included. These datasets will be a useful resource for researchers studying UC, and especially the influence of HDAC5 on epithelial-mesenchymal transition (EMT). Moreover, these data will inform studies on HDAC5 as a less studied member of the HDAC family in other cell types and diseases, especially fibrosis.
Perceptions and behavior of clinical researchers and research support staff regarding data FAIRification
Authors: Kersloot Martijn G. , Abu-Hanna Ameen , Cornet Ronald , Arts Derk L. 
Published: May 2022

AbstractThe FAIR Data Principles are being rapidly adopted by many research institutes and funders worldwide. This study aimed to assess the awareness and attitudes of clinical researchers and research support staff regarding data FAIRification. A questionnaire was distributed to researchers and support staff in six Dutch University Medical Centers and Electronic Data Capture platform users. 164 researchers and 21 support staff members completed the questionnaire. 62.8% of the researchers and 81.0% of the support staff are currently undertaking at least some effort to achieve any aspect of FAIR, 11.0% and 23.8%, respectively, address all aspects. Only 46.6% of the researchers add metadata to their datasets, 39.7% add metadata to data elements, and 35.9% deposit their data in a repository. 94.7% of the researchers are aware of the usefulness of their data being FAIR for others and 89.3% are, given the right resources and support, willing to FAIRify their data. Institutions and funders should, therefore, develop FAIRification training and tools and should (financially) support researchers and staff throughout the process.
Multi sequence average templates for aging and neurodegenerative disease populations
Authors: Dadar Mahsa , Camicioli Richard , Duchesne Simon 
Published: May 2022

AbstractMagnetic resonance image (MRI) processing pipelines use average templates to enable standardization of individual MRIs in a common space. MNI-ICBM152 is currently used as the standard template by most MRI processing tools. However, MNI-ICBM152 represents an average of 152 healthy young adult brains and is vastly different from brains of patients with neurodegenerative diseases. In those populations, extensive atrophy might cause inevitable registration errors when using an average template of young healthy individuals for standardization. Disease-specific templates that represent the anatomical characteristics of the populations can reduce such errors and improve downstream driven estimates. We present multi-sequence average templates for Alzheimer’s Dementia (AD), Fronto-temporal Dementia (FTD), Lewy Body Dementia (LBD), Mild Cognitive Impairment (MCI), cognitively intact and impaired Parkinson’s Disease patients (PD-CIE and PD-CI, respectively), individuals with Subjective Cognitive Impairment (SCI), AD with vascular contribution (V-AD), Vascular Mild Cognitive Impairment (V-MCI), Cognitively Intact Elderly (CIE) individuals, and a human phantom. We also provide separate templates for males and females to allow better representation of the diseases in each sex group.
Spatial and temporal data to study residential heat decarbonisation pathways in England and Wales
Authors: Canet Alexandre , Qadrdan Meysam , Jenkins Nick , Wu Jianzhong 
Published: May 2022

AbstractThe decarbonisation of residential heating is crucial if the net-zero target in the United Kingdom is going to be achieved. This paper describes methods to produce data to quantify the impacts of residential heat decarbonisation on the energy supply infrastructure across England and Wales. For the year 2018, annual heat demand for a range of dwellings was estimated for almost 35,000 local areas (known as Lower Layer Super Output Areas: LSOAs). Energy savings through implementing the potential energy efficiency measures and the indicative costs of the energy efficiency measures were quantified. Profiles were synthesised for heat production and energy demand of selected heating technologies using average daily temperature and data from trial projects. These profiles were created to study the impacts of different types of heating technology in each LSOA under user-defined heat decarbonisation pathways. Data describing the dwelling stock, heating technologies, annual heat demand for each LSOA, indicative costs of energy efficiency improvements for each local authority and the profiles for each technology were created.
Multi-cultural cities reduce disadvantages in recognizing naturalistic images of other-race faces: evidence from a novel face learning task
Authors: Zhou Xiaomei , Mondloch Catherine J. , Chien Sarina Hui-Lin , Moulson Margaret C. 
Published: May 2022

AbstractPeople often find it more difficult to recognize other- than own-race faces. This other-race effect is robust across numerous ethnic groups. Yet, it remains unclear how this effect changes in people who live in a multiracial environment, and in immigrants whose lifetime perceptual experience changes over time. In the present study, we developed a novel face recognition test that approximates face recognition in the real world. We tested five groups of White and East Asian adults (n = 120) living in racially homogeneous versus heterogeneous cities and East Asians who immigrated to a multiracial city between infancy and adulthood. Multiracial cities reduce the other-race effect. The magnitude of the other-race effect changes as a function of experience, mirroring the racial diversity in perceivers’ living environment. Our study highlights the challenge of forming reliable face representations across naturalistic facial variability and suggests a facilitative role of multiracial environments in eliminating the other-race effect.
The erythrocyte membrane properties of beta thalassaemia heterozygotes and their consequences for Plasmodium falciparum invasion
Authors: Introini Viola , Marin-Menendez Alejandro , Nettesheim Guilherme , Lin Yen-Chun , Kariuki Silvia N. , Smith Adrian L. , Jean Letitia , Brewin John N. , Rees David C. , Cicuta Pietro , Rayner Julian C. , Penman Bridget S. 
Published: May 2022

AbstractMalaria parasites such as Plasmodium falciparum have exerted formidable selective pressures on the human genome. Of the human genetic variants associated with malaria protection, beta thalassaemia (a haemoglobinopathy) was the earliest to be associated with malaria prevalence. However, the malaria protective properties of beta thalassaemic erythrocytes remain unclear. Here we studied the mechanics and surface protein expression of beta thalassaemia heterozygous erythrocytes, measured their susceptibility to P. falciparum invasion, and calculated the energy required for merozoites to invade them. We found invasion-relevant differences in beta thalassaemic cells versus matched controls, specifically: elevated membrane tension, reduced bending modulus, and higher levels of expression of the major invasion receptor basigin. However, these differences acted in opposition to each other with respect to their likely impact on invasion, and overall we did not observe beta thalassaemic cells to have lower P. falciparum invasion efficiency for any of the strains tested.
Children with autism spectrum disorder show atypical electroencephalographic response to processing contextual incongruencies
Authors: Márquez-García Amparo V. , Vakorin Vasily A. , Kozhemiako Nataliia , Magnuson Justine R. , Iarocci Grace , Ribary Urs , Moreno Sylvain , Doesburg Sam M. 
Published: May 2022

AbstractChildren with autism spectrum disorder (ASD) experience difficulties with social communication, making it challenging to interpret contextual information that aids in accurately interpreting language. To investigate how the brain processes the contextual information and how this is different in ASD, we compared event-related potentials (ERPs) in response to processing visual and auditory congruent and incongruent information. Two groups of children participated in the study: 37 typically developing children and 15 children with ASD (age range = 6 to 12). We applied a language task involving auditory sentences describing congruent or incongruent images. We investigated two ERP components associated with language processing: the N400 and P600. Our results showed how children with ASD present significant differences in their neural responses in comparison with the TD group, even when their reaction times and correct trials are not significantly different from the TD group.
Massive-mode polarization entangled biphoton frequency comb
Authors: Yamazaki Tomohiro , Ikuta Rikizo , Kobayashi Toshiki , Miki Shigehito , China Fumihiro , Terai Hirotaka , Imoto Nobuyuki , Yamamoto Takashi 
Published: May 2022

AbstractA frequency-multiplexed entangled photon pair and a high-dimensional hyperentangled photon pair are useful to realize a high-capacity quantum communication. A biphoton frequency comb (BFC) with entanglement can be used to prepare both states. We demonstrate polarization entangled BFCs with over 1400 frequency modes, which is approximately two orders of magnitude larger than those of earlier entangled BFCs, by placing a singly resonant periodically poled LiNbO3 waveguide resonator within a Sagnac loop. The BFCs are demonstrated by measuring the joint spectral intensity, cross-correlation, and autocorrelation. Moreover, the polarization entanglement at representative groups of frequency modes is verified by quantum state tomography, where each fidelity is over 0.7. The efficient generation of a massive-mode entangled BFC is expected to accelerate the increase of capacity in quantum communication.
Differences in composition of interdigital skin microbiota predict sheep and feet that develop footrot
Authors: Clifton Rachel , Monaghan Emma M. , Green Martin J. , Purdy Kevin J. , Green Laura E. 
Published: May 2022

AbstractFootrot has a major impact on health and productivity of sheep worldwide. The current paradigm for footrot pathogenesis is that physical damage to the interdigital skin (IDS) facilitates invasion of the essential pathogen Dichelobacter nodosus. The composition of the IDS microbiota is different in healthy and diseased feet, so an alternative hypothesis is that changes in the IDS microbiota facilitate footrot. We investigated the composition and diversity of the IDS microbiota of ten sheep, five that did develop footrot and five that did not (healthy) at weekly intervals for 20 weeks. The IDS microbiota was less diverse on sheep 2 + weeks before they developed footrot than on healthy sheep. This change could be explained by only seven of > 2000 bacterial taxa detected. The incubation period of footrot is 8–10 days, and there was a further reduction in microbial diversity on feet that developed footrot in that incubation period. We conclude that there are two stages of dysbiosis in footrot: the first predisposes sheep to footrot and the second occurs in feet during the incubation of footrot. These findings represent a step change in our understanding of the role of the IDS microbiota in footrot pathogenesis.
Effects of humic acid on Pb2+ adsorption onto polystyrene microplastics from spectroscopic analysis and site energy distribution analysis
Authors: Lu Xiaotian , Zeng Feng , Wei Shuyin , Gao Rui , Abdurahman Abliz , Wang Hao , Liang Weiqian 
Published: May 2022

AbstractMicroplastics (MPs), act as vectors of heavy metal pollutants in the environment, is of practical significance to study the adsorption process and mechanism on heavy metals. In this study, polystyrene microplastics (PSMPs) were used as model MPs to study the adsorption of Pb2+ on PSMPs and the effects of humic acid (HA) on the adsorption process. The results showed that HA promoted the adsorption of Pb2+ on PSMPs, and the higher the concentration of HA, the greater the adsorption of Pb2+. With the increase of pH value and decrease of ionic strength, the adsorption capacity of PSMPs for Pb2+ increased. The scanning electron microscope equipped with the energy dispersive spectroscope (SEM–EDS), fourier transform-infrared spectra (FT-IR) and X-ray photoelectron spectroscopy (XPS) analysis showed that Pb2+ could be adsorbed directly onto PSMPs and also indirectly by HA. The higher KSV values in the PSMPs-HA-Pb2+ system than PSMPs-HA system by fluorescence analysis of HA suggested that HA acted as a bridging role in the adsorption of Pb2+ on PSMPs. The site energy distribution analysis further revealed that HA increased the average site energy μ(E*) and its standard deviation σe* of PSMPs by introducing more adsorption sites, thus enhanced the adsorption affinity of PSMPs. This study provided more thoughts and insights into the adsorption behavior and mechanism of MPs for Pb2+ in aquatic environments.
A cross-sectional survey on attitudes of men who have sex with men towards anal self-examination for detection of anal syphilis
Authors: Aung Ei T. , Fairley Christopher K. , Ong Jason J. , Phillips Tiffany R. , Chen Marcus Y. , Tran Julien , Maddaford Kate , Rodriguez Elena R. , Chow Eric P. F. 
Published: May 2022

AbstractStudies suggest men who have sex with men (MSM) practising receptive anal sex are more likely to present with secondary syphilis, implying primary anorectal lesions are likely to be missed. If men could detect anorectal lesions in the primary stage by regular anal self-examination (ASE), transmission could be reduced by early diagnosis and treatment. We aimed to explore the attitudes of MSM on performing ASE to detect primary anorectal syphilis. An online anonymous cross-sectional survey among MSM over 18 years of age living in Australia, was conducted between July and November 2020 and recruitment was from a sexual health clinic and social media. A total of 568 MSM completed the survey (median age: 34 [IQR 27–45]): 32% (183) had previously performed ASE. Among 66% (374) who had never performed ASE, 68% (250) would consider performing ASE in the future with a preferred median frequency of 2 times per 4 weeks (IQR 1–4), whilst men who were already performing ASE were performing it at median 1 per 4 weeks (IQR 0.2–3). Almost two-thirds of MSM who had never performed ASE were willing to adopt ASE practice in the future. Studies are required to determine the effectiveness of ASE for syphilis detection.
An evaluation of Internet searches as a marker of trends in population mental health in the US
Authors: Vaidyanathan Uma , Sun Yuantong , Shekel Tomer , Chou Katherine , Galea Sandro , Gabrilovich Evgeniy , Wellenius Gregory A. 
Published: May 2022

AbstractThe absence of continuous, real-time mental health assessment has made it challenging to quantify the impacts of the COVID-19 pandemic on population mental health. We examined publicly available, anonymized, aggregated data on weekly trends in Google searches related to anxiety, depression, and suicidal ideation from 2018 to 2020 in the US. We correlated these trends with (1) emergency department (ED) visits for mental health problems and suicide attempts, and (2) surveys of self-reported symptoms of anxiety, depression, and mental health care use. Search queries related to anxiety, depression, and suicidal ideation decreased sharply around March 2020, returning to pre-pandemic levels by summer 2020. Searches related to depression were correlated with the proportion of individuals reporting receiving therapy (r = 0.73), taking medication (r = 0.62) and having unmet mental healthcare needs (r = 0.57) on US Census Household Pulse Survey and modestly correlated with rates of ED visits for mental health conditions. Results were similar when considering instead searches for anxiety. Searches for suicidal ideation did not correlate with external variables. These results suggest aggregated data on Internet searches can provide timely and continuous insights into population mental health and complement other existing tools in this domain.
Estimation of biological effect of Cu-64 radiopharmaceuticals with Geant4-DNA simulation
Authors: Kusumoto Tamon , Baba Kentaro , Hasegawa Sumitaka , Raffy Quentin , Kodaira Satoshi 
Published: May 2022

AbstractThe aim of this work is to estimate the biological effect of targeted radionuclide therapy using Cu-64, which is a well-known Auger electron emitter. To do so, we evaluate the absorbed dose of emitted particles from Cu-64 using the Geant4-DNA Monte Carlo simulation toolkit. The contribution of beta particles to the absorbed dose is higher than that of Auger electrons. The simulation result agrees with experimental ones evaluated using coumarin-3-carboxylic acid chemical dosimeter. The simulation result is also in good agreement with previous ones obtained using fluorescent nuclear track detector. From the results of present simulation (i.e., absorbed dose estimation) and previous biological experiments using two cell lines (i.e., evaluation of survival curves), we have estimated the relative biological effectiveness (RBE) of Cu-64 emitted particles on CHO wild-type cells and xrs5 cells. The RBE of xrs5 cells exposed to Cu-64 is almost equivalent to that with gamma rays and protons and C ions. This result indicates that the radiosensitivity of xrs5 cells is independent of LET. In comparison to this, the RBE on CHO wild-type cells exposed to Cu-64 is significantly higher than gamma rays and almost equivalent to that irradiated with C ions with a linear energy transfer of 70 keV/μm.
Stack effects in tall building fires: a case study of Taiwan old apartment fire
Authors: Huang HongSheng , Lin ChingYuan , Wang ShiuanCheng , Su ChungHwei , Chen LiPeng 
Published: May 2022

AbstractTainan, a city that prospered early in Taiwan, has a hot and humid atmosphere. Hence, the grilled doors in numerous old buildings for ventilation and lighting to conserve energy. This study analyzed a fire incident that occurred during the late night of March 17, 2019 in a 38-year-old dwelling, where three residents were severely covered with soot. The site investigation showed that eight staircases lead to the same basement, which apparently created a stack effect and a makeup air phenomenon. Numerical simulations have been performed in this study to reconstruct the fire scene, whose results were consistent with the actual fire scene. In particular, the results showed that some staircases in the fire were blackened by smoke, while others acted as makeup air inlets. The temperature at the households’ doors on all floors of Staircase 2, which was closest to the fire, exceeded 60 °C after four minutes. Furthermore, two immediately feasible improvement strategies according to the control volume theory of fluid mechanics were proposed in this study. Firstly, changing the grilled doors in the basement to a closed flat door style could effectively prevent smoke from flowing up in the staircases. Secondly, residents may consider closing the windows of the stairs at night to improve fire safety. The results showed that the chimney effect can be significantly reduced. These improvements could be a reference for other old dwellings to enhance their fire safety.