bluuebunny/crossref_metadata_2025_split huggingface dataset:
Childhood pertussis is still here: An Asian city's perspectives
Authors: Hon Kam Lun E. , Li Yee Ming Jennifer , Leung Alexander K. C. , Wong Alex H. C. , Leung Karen Ka Yan , Ng Daniel K. K. 
Published: January 2022

AbstractIntroductionPertussis, or whooping cough, is a highly contagious respiratory infection that is caused by the bacterium Bordetella pertussis. It is one of the most common causes of death in childhood. It is also a frequent cause of chronic cough in children, adolescents, and adults.MethodsGlobal and Hong Kong perspectives of childhood pertussis were described.ResultsHong Kong has prided herself in the city's childhood immunization program. There appear to be no major outbreaks of pertussis since the 1960s. Nevertheless, pediatricians may see isolated cases of pertussis or pertussis‐like cases from time to time. Occasionally, infants are severely affected with apneas and managed with ventilator supports in the PICU. Outbreaks of the notifiable disease continue to occur despite a reasonable surveillance system and vaccination program in Hong Kong. Vaccination of mothers, adolescents, and adults are efficacious methods to further reduce the risks of pertussis. Macrolides remain efficacious antibiotics especially used early during the infectious phase. Infants with pertussis may require intensive care support and morbidity is high.ConclusionsPhysicians should be reminded from time to time that outbreaks of pertussis still exist in Hong Kong and in many cities globally.
Redefining the coenzyme A transferase superfamily with a large set of manually annotated proteins
Authors: Hackmann Timothy J. 
Published: January 2022

AbstractThe coenzyme A (CoA) transferases are a superfamily of proteins central to the metabolism of acetyl‐CoA and other CoA thioesters. They are diverse group, catalyzing over a 100 biochemical reactions and spanning all three domains of life. A deeply rooted idea, proposed two decades ago, is these enzymes fall into three families (I, II, and III). Here we find they fall into different families, which we achieve by analyzing all CoA transferases characterized to date. We manually annotated 94 CoA transferases with functional information (including rates of catalysis for 208 reactions) from 97 publications. This represents all enzymes we could find in the primary literature, and it is double the number annotated in four protein databases (BRENDA, KEGG, MetaCyc, UniProt). We found family I transferases are not closely related to each other in terms of sequence, structure, and reactions catalyzed. This family is not even monophyletic. These problems are solved by regrouping the three families into six, including one family with many non‐CoA transferases. The problem (and solution) became apparent only by analyzing our large set of manually annotated proteins. It would have been missed if we had used the small number of proteins annotated in UniProt and other databases. Our work is important to understanding the biology of CoA transferases. It also warns investigators doing phylogenetic analyses of proteins to go beyond information in databases.
Model‐based event‐triggered control of singularly perturbed system with dual event‐triggering mechanism
Authors: Bhandari Manisha , Fulwani Deepak , Gupta Rajeev 
Published: January 2022

AbstractIn this article, a new dual event‐triggering mechanism is proposed to implement a distributed model‐based event‐triggered control for a singularly perturbed system (SPS) having structured state space uncertainty. The proposed approach significantly reduces the amount of necessary information exchanged to control the system. Dual event‐triggering mechanism allows the measurements of slow and fast states to be transmitted asynchronously at different rates which is more effective due to the two time‐scale nature of SPSs. Approximate slow and fast systems of the nominal plant are used as the model to reduce the computational demand. The proposed control strategy guarantees that the system states converge to an adjustable region around the equilibrium point and the inter‐event intervals are lower‐bounded by a positive constant. A numerical simulation example is used to illustrate the theoretical results of the article.
Patchiness in flow refugia use by macroinvertebrates following an artificial flood pulse
Authors: Mathers Kate L. , Robinson Christopher T. , Weber Christine 
Published: January 2022

AbstractFlow refugia, locations that maintain substrate stability and low hydraulic stress during periods of high flow, can ensure riverine resilience in the face of increasing hydrological unpredictability. Despite their known importance, they have been overlooked in recent years with work on drought refugia currently seeing greater attention. Moreover, research on the role of flow refugia during artificial flood pulses in regulated rivers, where flood disturbances are no longer part of the hydrograph, is essentially absent. Here, we compared flow refugia for benthic macroinvertebrates among six habitats (main channel, side channel, riffle, margin, lentic including a floodplain pond, and inundated floodplain) within four different sites in response to an artificial flood pulse. We found that the grain‐size distribution and macroinvertebrate community composition changed at each site following the flood. Macroinvertebrate assemblages became longitudinally homogeneous, but within‐site beta diversity and taxa richness remained temporally stable following the flood pulse, suggesting the presence of flow refugia. In this respect, margin, inundated floodplain and lentic (a floodplain pond) habitats provided important flow refugia locations, particularly for the mobile mayfly Rhithrogena sp. In contrast, low substrate stability in riffle and side channels resulted in limited refugia potential for most taxa. Refuge use was however patchy with high levels of intra‐habitat variability being evident for Rhithrogena sp. and the amphipod Gammarus fossarum in margin and side channel habitats. Further work is required to advance our knowledge of flow refugia in rivers with differing flow regimes to enable their integration into management and restoration schemes.
CarbonIron Electron Transport Channels in Porphyrin–Graphene Complex for ppb‐Level Room Temperature NO Gas Sensing
Authors: Gao Yixun , Wang Jianqiang , Feng Yancong , Cao Nengjie , Li Hao , de Rooij Nicolaas Frans , Umar Ahmad , French Paddy J. , Wang Yao , Zhou Guofu 
Published: January 2022

AbstractIt is a great challenge to develop efficient room‐temperature sensing materials and sensors for nitric oxide (NO) gas, which is a biomarker molecule used in the monitoring of inflammatory respiratory diseases. Herein, Hemin (Fe (III)‐protoporphyrin IX) is introduced into the nitrogen‐doped reduced graphene oxide (N‐rGO) to obtain a novel sensing material HNG‐ethanol. Detailed XPS spectra and DFT calculations confirm the formation of carbon–iron bonds in HNG‐ethanol during synthesis process, which act as electron transport channels from graphene to Hemin. Owing to this unique chemical structure, HNG‐ethanol exhibits superior gas sensing properties toward NO gas (Ra/Rg = 3.05, 20 ppm) with a practical limit of detection (LOD) of 500 ppb and reliable repeatability (over 5 cycles). The HNG‐ethanol sensor also possesses high selectivity against other exhaled gases, high humidity resistance, and stability (less than 3% decrease over 30 days). In addition, a deep understanding of the gas sensing mechanisms is proposed for the first time in this work, which is instructive to the community for fabricating sensing materials based on graphene‐iron derivatives in the future.
Landslide detection using deep learning and object-based image analysis
Authors: Ghorbanzadeh Omid , Shahabi Hejar , Crivellari Alessandro , Homayouni Saeid , Blaschke Thomas , Ghamisi Pedram 
Published: January 2022

AbstractRecent landslide detection studies have focused on pixel-based deep learning (DL) approaches. In contrast, intuitive annotation of landslides from satellite imagery is based on distinct features rather than individual pixels. This study examines the feasibility of the integration framework of a DL model with rule-based object-based image analysis (OBIA) to detect landslides. First, we designed a ResU-Net model and then trained and tested it in the Sentinel-2 imagery. Then we developed a simple rule-based OBIA with only four rulesets, applying it first to the original image dataset and then to the same dataset plus the resulting ResU-Net heatmap. The value of each pixel in the heatmap refers to the probability that the pixel belongs to either landslide or non-landslide classes. Thus, we evaluate three scenarios: ResU-Net, OBIA, and ResU-Net-OBIA. The landslide detection maps from three different classification scenarios were compared against a manual landslide inventory map using thematic accuracy assessment metrics: precision, recall, and f1-score. Our experiments in the testing area showed that the proposed integration framework yields f1-score values 8 and 22 percentage points higher than those of the ResU-Net and OBIA approaches, respectively.
Effects of PM2.5 and its constituents on hemoglobin during the third trimester in pregnant women
Authors: Xie Guilan , Yue Jie , Yang Wenfang , Yang Liren , Xu Mengmeng , Sun Landi , Zhang Boxing , Guo Leqian , Chung Mei Chun 
Published: January 2022

AbstractAnemia has been a public health issue evoking global concern, and the low hemoglobin (Hb) concentration links to adverse pregnancy outcomes. However, the associations of PM2.5 and its constituents with Hb and anemia in pregnant women remain unclear. In this retrospective birth cohort study, 7932 pregnant women who delivered in the First Affiliated Hospital of Xi’an Jiaotong University from 2015 to 2018 were included. The Hb during the third trimester in pregnant women was assessed before delivery. PM2.5 and its constituents (BC, NH4+, NO3−, OM, SO42−, and Dust) during pregnancy were retrieved from the V4.CH.03 product constructed by the Atmospheric Composition Analysis Group. Generalized linear regression model was applied to investigate the effects of PM2.5 and its constituents on Hb and anemia during the third trimester in pregnant women. The means and standard deviations of PM2.5, BC, NH4+, NO3−, OM, SO42−, and Dust were 69.56 (15.24), 10.02 (2.72), 8.11 (1.77), 14.96 (5.42), 15.36 (4.11), 10.08 (1.20), and 10.98 (1.85) μg/m3, respectively. Per IQR increase (μg/m3) of PM2.5, BC, NO3−, and OM linked to − 0.75 (− 1.50, − 0.01), − 0.85 (− 1.65, − 0.04), − 0.79 (− 1.56, − 0.03), and − 0.73 (− 1.44, − 0.03) g/L decrease of Hb during the third trimester in multiparous pregnant women, but not for NH4+, SO42−, Dust, and primiparous pregnant women. PM2.5 and its constituents had no significant association with anemia, except for Dust (OR: 0.90, 95% CI: 0.82, 0.99, per IQR increase) in primiparous pregnant women. Besides, SO42− was of lag effects on Hb and anemia in multiparous pregnant women. Moreover, non-linear associations were found among PM2.5 and its constituents, Hb, and anemia. Therefore, exposure to PM2.5 and some constituents of PM2.5 was associated with reduced Hb level during the third trimester in multiparous pregnant women. Related departments and pregnant women should take targeted actions to eliminate the detrimental effects of PM2.5 and its constituents on pregnancy outcomes.
SOX2 knockdown with siRNA reverses cisplatin resistance in NSCLC by regulating APE1 signaling
Authors: Chen Tai-yu , Zhou Ji , Li Peng-cheng , Tang Chun-han , Xu Ke , Li Tao , Ren Tao 
Published: January 2022

AbstractSOX2 is related to drug resistance in many types of cancer, including lung cancer. Herein, we investigated the role of SOX2 and its regulatory signaling in cisplatin-treated non-small-cell lung cancer (NSCLC). The effects of SOX2 on cell viability, proliferation, and apoptosis were evaluated in vitro. Western blotting, real-time quantitative PCR, immunohistochemistry, and luciferase reporter assays were used to investigate the underlying mechanism. Kaplan–Meier survival analysis and the log-rank test were used to assess the relationship between SOX2 expression and patient survival. A549/CDDP cells had marked resistance to cisplatin and stronger colony formation ability than A549 cells. The expression of SOX2 protein or mRNA in A549/CDDP was higher than that in A549. Knockdown of SOX2 in A549/CDDP-induced apoptosis by inhibiting colony formation and decreasing viability, but overexpression of SOX2 reversed these effects. Interestingly, Genomatix software predicted that the APE1 promoter has some SOX2 binding sites, while the SOX2 promoter has no APE1 binding sites. Furthermore, luciferase reporter assays proved that SOX2 could bind the promoter of APE1 in 293T cells. We further verified that SOX2 expression was not affected by shAPE1 in A549/CDDP. As expected, colony formation was obviously inhibited and apoptosis was strongly enhanced in A549/CDDP treated with SOX2 siSOX2 alone or combined with CDDP compared with control cells. Meaningfully, patients with low expression of SOX2, and even including its regulating APE1, survived longer than those with high expression of SOX2, and APE1. siSOX2 overcomes cisplatin resistance by regulating APE1 signaling, providing a new target for overcoming cisplatin resistance in NSCLC.
Non-clinical Pharmacology of YTX-7739: a Clinical Stage Stearoyl-CoA Desaturase Inhibitor Being Developed for Parkinson’s Disease
Authors: Tardiff Daniel F. , Lucas Matthew , Wrona Iwona , Chang Belle , Chung Chee Yeun , Le Bourdonnec Bertrand , Rhodes Kenneth J. , Scannevin Robert H. 
Published: January 2022

Summary
Stearoyl-CoA desaturase (SCD) is a potential therapeutic target for Parkinson’s and related neurodegenerative diseases. SCD inhibition ameliorates neuronal toxicity caused by aberrant α-synuclein, a lipid-binding protein implicated in Parkinson’s disease. Its inhibition depletes monounsaturated fatty acids, which may modulate α-synuclein conformations and membrane interactions. Herein, we characterize the pharmacokinetic and pharmacodynamic properties of YTX-7739, a clinical-stage SCD inhibitor. Administration of YTX-7739 to rats and monkeys for 15 days caused a dose-dependent increase in YTX-7739 concentrations that were well-tolerated and associated with concentration-dependent reductions in the fatty acid desaturation index (FADI), the ratio of monounsaturated to saturated fatty acids. An approximate 50% maximal reduction in the carbon-16 desaturation index was observed in the brain, with comparable responses in the plasma and skin. A study with a diet supplemented in SCD products indicates that changes in brain C16 desaturation were due to local SCD inhibition, rather than to changes in systemic fatty acids that reach the brain. Assessment of pharmacodynamic response onset and reversibility kinetics indicated that approximately 7 days of dosing were required to achieve maximal responses, which persisted for at least 2 days after cessation of dosing. YTX-7739 thus achieved sufficient concentrations in the brain to inhibit SCD and produce pharmacodynamic responses that were well-tolerated in rats and monkeys. These results provide a framework for evaluating YTX-7739 pharmacology clinically as a disease-modifying therapy to treat synucleinopathies.
The contribution of fisheries and aquaculture to the global protein supply
Authors: Boyd Claude E. , McNevin Aaron A. , Davis Robert P. 
Published: January 2022

AbstractThe contribution of aquatic animal protein to the global, animal-source protein supply and the relative importance of aquaculture to capture fisheries in supplying this protein is relevant in assessments and decisions related to the future of aquatic food production and its security. Meat of terrestrial animals, milk, and eggs resulted in 76,966 Kt crude protein compared with 13,950 Kt or 15.3% from aquatic animals in 2018.While aquaculture produced a greater tonnage of aquatic animals, capture fisheries resulted in 7,135 Kt crude protein while aquaculture yielded 6,815 Kt. Capture fisheries production has not increased in the past two decades, and aquaculture production must increase to assure the growing demand for fisheries products by a larger and more affluent population. We estimated based onstatus quoconsumption, that aquaculture production would need to increase from 82,087 Kt in 2018 to 129,000 Kt by 2050 to meet the demand of the greater population. About two-thirds of finfish and crustacean production by aquaculture is feed-based, and feeds for these species include fishmeal and fish oil as ingredients. Aquaculture feeds require a major portion of the global supply of fishmeal and fish oil. An estimated 71.0% of fishmeal and 73.9% of fish oil are made from the catch with the rest coming from aquatic animal processing waste. The catch of small, pelagic fish from the ocean is not predicted to increase in the future. Aquaculture should reduce its fishmeal and oil use to lessen its dependency on small wild fish important to the integrity of marine food webs and food security for the poor in many coastal areas. Fishmeal and fish oil shortages for use in aquaculture feed will result in a limit on production in the future if goals to lessen their use in feeds are not met.
Novel Dithiolene Nickel Complex Catalysts for Electrochemical Hydrogen Evolution Reaction for Hydrogen Production in Nonaqueous and Aqueous Solutions
Authors: Niu Zhicheng , Yang Lemin , Xiao Yongxin , Xue Min , Zhou Jiarong , Zhang Lei , Zhang Jiujun , Wilkinson David P. , Ni Chunlin 
Published: January 2022

AbstractThree molecular catalysts based on mononuclear nickel(II) complexes with square planar geometries, [BzPy]2[Ni(mnt)2] (1), [BzPy]2[Ni(i-mnt)2] (2), and [BzPy]2[Ni(tdas)2] (3) (BzPy = benzyl pyridinium) are synthesized by the reaction of NiCl2∙6H2O, [BzPy]Br, and Na2(mnt)/Na2(i-mnt)/Na2(tdas) (mnt = 1,2-dicyanoethylene-1,2-dithiolate for (1), i-mnt = 2,2-dicyanoethylene-1,1-dithiolate for (2), and tdas = 1,2,5-thiadiazole-3,4-dithiolate for (3)), respectively. The structures and compositions of these three catalysts are characterized by XRD, elemental analysis, FT-IR, and ESI-MS. The electrochemical properties and the corresponding catalytic activities of these three catalysts are studied by cyclic voltammetry. The controlled-potential electrolysis with gas chromatography analysis confirms the hydrogen production with a turnover frequency (TOF) of 116.89, 165.51, and 189.16 moles of H2per mole of catalyst per hour at a potential of − 0.99 V (versus SHE) in acetonitrile solutions containing the catalysts, respectively. In a neutral buffer solution, these three molecular catalysts exhibit a TOF of 411.85, 488.76, and 555.06 mol of H2per mole of catalyst per hour at a potential of − 0.49 V (versus SHE), respectively, indicating that Complex3constitutes the better active catalyst than Complexes1and2. For fundamental understanding, a catalytic HER mechanism is also proposed.Graphical abstract
Evidence of glucose absorption in a neoformed intestine
Authors: Tuveri Massimiliano , Paiella Salvatore , Boschi Federico , Luchini Claudio , Perri Giampaolo , Gasparini Clizia , Aresta Alex , Scarpa Aldo , Salvia Roberto , Bassi Claudio 
Published: January 2022

AbstractRecent advances in the field of tissue regeneration are offering promising therapeutic options for the treatment of short bowel syndrome. This study aimed to evaluate the glucose absorptive capacity of a neoformed intestine obtained from a biological scaffold in a rodent model and the steadiness of the engrafted segment area. Twenty-four male Sprague–Dawley rats were used for this study. Under anesthesia, a patch of biological material (2.2 × 1.5 cm) was engrafted in the anti-mesenteric border of the small bowels of 12 rats. Twelve rats were sham-operated. Animals were studied at 4, 8, and 10 months postengraftment. Functional and histological analyses were performed. The functional analysis was performed using an 18F-FDG analog as a probe and the results were acquired with an optical imager. The intensity of the fluorescent signal emitted by the neointestine was comparable with that emitted by the native intestine in all animals and was visible after injection in the preserved mesentery. The mean intestinal volume at time of engraftment and after 10 months was 4.08 cm3 (95% CI [3.58–4.58]) and 3.26 cm3 (CI 95% [3.23–3.29]), respectively, with a mean shrinkage of 17.3% (range 10.6–23.8%), without any evidence of stenosis. Morphological analysis revealed the progression of the biological material toward a neoformed intestine similar to the native intestine, especially at 8 and 10 months. In a rodent model, we demonstrated that a neointestine, obtained from a biological scaffold showed glucose absorption and a durable increase in diameter.
What Exactly is it that the Taiwan Greens Want? Extracting “Taiwan Subjectivity” from the Liberty Times Newspaper
Authors: Lynch Daniel C. , Yau Cody Wai-kwok 
Published: January 2022

AbstractOne source of the idea that Taiwan independence would be politically desirable is belief in the concept of “Taiwan subjectivity,” which indicates that Taiwan is not an appendage of China but instead an autonomous actor charting its own course – or trying to do so in the face of huge difficulties. The ruling (since 2016) Democratic Progressive Party (DPP) pledges fealty to the goal of ultimately realizing subjectivity but cannot aggressively pursue the agenda because of opposition from the People's Republic of China (PRC), the United States, and some in Taiwan itself. What might that agenda be? Using a Structural Topic Model, we excavate the subjectivity discourse as it developed from 2008 to 2020 in the mainstream DPP-supporting newspaper, the Liberty Times. We find fourteen topics associated with the concept, the most prevalent of which in recent years warn of threats to subjectivity's realization in the political and sociocultural spheres.
Doctors as Appointed Fiduciaries: A Supplemental Model for Medical Decision-Making
Authors: Davies Ben , Parker Joshua 
Published: January 2022

AbstractHow should we respond to patients who do not wish to take on the responsibility and burdens of making decisions about their own care? In this paper, we argue that existing models of decision-making in modern healthcare are ill-equipped to cope with such patients and should be supplemented by an “appointed fiduciary” model where decision-making authority is formally transferred to a medical professional. Healthcare decisions are often complex and for patients can come at time of vulnerability. While this does not undermine their capacity, it can be excessively burdensome. Most existing models of decision-making mandate that patients with capacity must retain ultimate responsibility for decisions. An appointed fiduciary model provides a formalized mechanism through which those few patients who wish to defer responsibility can hand over decision-making authority. By providing a formal structure for deferring to an appointed fiduciary, the confusions and risks of the informal transfers that can occur in practice are avoided. Finally, we note how appropriate governance and law can provide safeguards against risks to the welfare of patients and medical professionals.