bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary Meat, Trimethylamine N-Oxide-Related Metabolites, and Incident Cardiovascular Disease Among Older Adults: The Cardiovascular Health Study
Authors: Wang Meng , Wang Zeneng , Lee Yujin , Lai Heidi T.M. , de Oliveira Otto Marcia C. , Lemaitre Rozenn N. , Fretts Amanda , Sotoodehnia Nona , Budoff Matthew , DiDonato Joseph A. , McKnight Barbara , Tang W.H. Wilson , Psaty Bruce M. , Siscovick David S. , Hazen Stanley L. , Mozaffarian Dariush 
Published: August 2022

Background:
Effects of animal source foods (ASF) on atherosclerotic cardiovascular disease (ASCVD) and underlying mechanisms remain controversial. We investigated prospective associations of different ASF with incident ASCVD and potential mediation by gut microbiota-generated trimethylamine N-oxide, its L-carnitine-derived intermediates γ-butyrobetaine and crotonobetaine, and traditional ASCVD risk pathways.

Methods:
Among 3931 participants from a community-based US cohort aged 65+ years, ASF intakes and trimethylamine N-oxide-related metabolites were measured serially over time. Incident ASCVD (myocardial infarction, fatal coronary heart disease, stroke, other atherosclerotic death) was adjudicated over 12.5 years median follow-up. Cox proportional hazards models with time-varying exposures and covariates examined ASF-ASCVD associations; and additive hazard models, mediation proportions by different risk pathways.

Results:
After multivariable-adjustment, higher intakes of unprocessed red meat, total meat, and total ASF associated with higher ASCVD risk, with hazard ratios (95% CI) per interquintile range of 1.15 (1.01–1.30), 1.22 (1.07–1.39), and 1.18 (1.03–1.34), respectively. Trimethylamine N-oxide-related metabolites together significantly mediated these associations, with mediation proportions (95% CI) of 10.6% (1.0–114.5), 7.8% (1.0–32.7), and 9.2% (2.2–44.5), respectively. Processed meat intake associated with a nonsignificant trend toward higher ASCVD (1.11 [0.98–1.25]); intakes of fish, poultry, and eggs were not significantly associated. Among other risk pathways, blood glucose, insulin, and C-reactive protein, but not blood pressure or blood cholesterol, each significantly mediated the total meat-ASCVD association.

Conclusions:
In this large, community-based cohort, higher meat intake associated with incident ASCVD, partly mediated by microbiota-derived metabolites of L-carnitine, abundant in red meat. These novel findings support biochemical links between dietary meat, gut microbiome pathways, and ASCVD.
Clinical Correlates and Outcomes of Methamphetamine‐Associated Cardiovascular Diseases in Hospitalized Patients in California
Authors: Curran Lara , Nah Gregory , Marcus Gregory M. , Tseng Zian , Crawford Michael H. , Parikh Nisha I. 
Published: August 2022

Background
Methamphetamine misuse affects 27 million people worldwide and is associated with cardiovascular disease (CVD); however, risk factors for CVD among users have not been well studied.

Methods and Results
We studied hospitalized patients in California, captured by the Healthcare Cost and Utilization Project database, between 2005 and 2011. We studied the association between methamphetamine use and CVD (pulmonary hypertension, heart failure, stroke, and myocardial infarction). Among 20 249 026 persons in the Healthcare Cost and Utilization Project, 66 199 used methamphetamines (median follow‐up 4.58 years). Those who used were more likely younger (33 years versus 45 years), male (63.3% versus 44.4%), smoked, misused alcohol, and had depression and anxiety compared with nonusers. Methamphetamine use was associated with the development of heart failure (hazard ratio [HR], 1.53 [95% CI, 1.45–1.62]) and pulmonary hypertension (HR, 1.42 [95% CI, 1.26–1.60]). Among users, male sex (HR, 1.73 [95% CI, 1.37–2.18]) was associated with myocardial infarction. Chronic kidney disease (HR, 2.38 [95% CI, 1.74–3.25]) and hypertension (HR, 2.26 [95% CI, 2.03–2.51]) were strong risk factors for CVD among users. When compared with nonuse, methamphetamine use was associated with a 32% significant increase in CVD, alcohol abuse with a 28% increase, and cocaine use with a 47% increase in CVD.

Conclusions
Methamphetamine use has a similar magnitude of risk of CVD compared with alcohol and cocaine. Prevention and treatment could be focused on those with chronic kidney disease, hypertension, and mental health disorders.
Evaluation of CATCHVIEW Versus Standard Stent Retrievers for Endovascular Therapy: Results From the ETIS Registry
Authors: Maïer Benjamin , Blanc Raphaël , Labreuche Julien , Piotin Michel , Gory Benjamin , Bourcier Romain , Marnat Gaultier , Consoli Arturo , Lapergue Bertrand ,   the ETIS registry
Published: August 2022

Background
The CATCHVIEW device (Balt) is a last‐generation stent retriever designed for endovascular therapy. We aimed to compare the CATCHVIEW device with standard stent retrievers (SSRs) using propensity score methods and assess the noninferiority of CATCHVIEW versus SSRs.

Methods
We used the ETIS (Endovascular Treatment in Ischemic Stroke) multicenter (13 centers), prospective registry to compare clinical and radiological data of patients treated with CATCHVIEW or SSRs as a first‐line strategy. Procedural outcomes (successful, near‐perfect, perfect reperfusion after first‐line strategy, and at the end of endovascular therapy), clinical outcomes (modified Rankin Scale at 90 days), and safety outcomes (symptomatic intracranial hemorrhage, mortality at 90 days) were compared after propensity score matching. Noninferiority of CATCHVIEW was established if the prespecified lower bound of the 95% CI was >−10%.

Results

From March 2017 to March 2020, 171 patients treated first‐line by CATCHVIEW were matched to 617 patients treated first‐line by an SSR. In the propensity score–matched cohort, successful reperfusion after first‐line strategy was achieved in 73.9% and 76.2% in the CATCHVIEW and SSR groups, respectively (absolute difference, −2.3%; 1‐sided 95% CI, −7.0% to
∞
), demonstrating noninferiority of CATCHVIEW versus SSR. CATCHVIEW was associated with better near‐perfect (matched relative risk, 1.15; [95% CI, 1.00–1.31]) and perfect (matched relative risk, 1.26; [95% CI, 1.07–1.47]) reperfusion rates at the end of endovascular therapy and favorable 90‐day outcomes (matched relative risk, 1.27; [95% CI, 1.03–1.55]). Safety outcomes were comparable.

Conclusions
In this propensity score–matched cohort, we demonstrated the noninferiority of CATCHVIEW compared with SSR, as the first‐line strategy for successful reperfusion. CATCHVIEW achieved higher reperfusion rates at the end of endovascular therapy and higher rates of favorable outcomes at 90 days.
Bilateral Alterations in Isokinetic Strength and Knee Biomechanics During Side-Cutting 1 Year After Unilateral ACL Reconstruction
Authors: Shi Huijuan , Ren Shuang , Huang Hongshi , Liu Hui , Liang Zixuan , Yu Yuanyuan , Li Hanjun , Ao Yingfang 
Published: August 2022

Background: Individuals with anterior cruciate ligament (ACL) reconstruction (ACLR) are a population that has a higher risk for ACL injury compared with the general population. To reduce the reinjury rate and improve the rehabilitation outcome after ACLR, risk factors for ACL injury have to be addressed. Purpose: To compare the knee biomechanics during side-cutting and isokinetic strength of the thigh muscle of the reconstructed leg with those of the contralateral leg and healthy controls and investigate the knee movement asymmetries in individuals with ACLR. Study Design: Controlled laboratory study. Methods: A total of 16 participants with ACLR (ACLR group; 11.8 ± 1.1 months after reconstruction) and 16 healthy controls (control group) were recruited. Landmark coordinates and ground-reaction forces during side-cutting and isokinetic strength of hamstring and quadriceps were collected. Two-way analysis of variance with the mixed design was performed to compare each dependent variable between groups and across legs. Results: The reconstructed leg had a significantly smaller knee flexion angle ( P = .004) and less quadriceps strength ( P = .003) than the contralateral leg. The knee extension moment and knee external rotation angle were decreased compared with both the contralateral leg ( P = .001, P = .003, respectively) and the healthy control leg ( P = .001, P = .001, respectively). The ACLR group showed greater knee abduction angles ( P = .004) and smaller knee external rotation moments ( P = .006) than the control group. The ACLR group also demonstrated greater asymmetries of knee flexion angle ( P = .015), knee external rotation angle ( P = .001), knee extension moment ( P = .013), knee abduction moment ( P = .001), and quadriceps strength ( P = .046) than the control group. Conclusion: Knee biomechanics in the leg with ACLR were altered mainly in the sagittal plane during side-cutting compared with the contralateral leg. The altered movement patterns between the ACLR and control groups were primarily observed in the frontal and transverse planes. The ACLR group also demonstrated greater asymmetries of sagittal knee movement and concentric quadriceps strength than the control group. Clinical Relevance: Individuals with ACLR showed different alterations in the reconstructed and contralateral leg compared with healthy controls. These results suggest that rehabilitation programs after ACLR should further focus on restoring the knee flexion angle and quadriceps strength. Injury prevention programs need to be further targeted in the altered movement patterns observed between the ACLR and the healthy groups.
Prevalence and Features of Maxillary Sinus Septa in Patients with Cleft Lip and Palate: Cone Beam Computed Tomography Imaging Technique
Authors: Paknahad Maryam , Zahedrozegar Samira , Babanouri Neda , Ajami Shabnam 
Published: August 2022

Objective The development of the maxillary sinus is different in patients with cleft lip and palate (CLP) compared to non-CLP individuals. To investigate the prevalence and features of maxillary sinus septa (MSS) in patients with CLP in comparison with the non-CLP population. Design Retrospective study. Intervention Cone beam computed tomography (CBCT) evaluation. Setting CLP center in Shiraz faculty of dentistry, Iran. Patients A total 306 sinuses (88 cleft and 218 noncleft) on 153 images (CLP group: n  =  66; control group: n  =  87) were examined to determine the prevalence of septa and characterize them. Main Outcome Measures Sinus septa were characterized according to height, orientation, angle, origin, and location. The chi-square test, Mann-Whitney U test, and Fisher's exact test were used for statistical analysis. Results The prevalence of septa was 28.9% and 32.1% in the CLP and control groups, respectively. No significant difference was found between the study groups in terms of prevalence, location, and orientation of MSS. The average height and angle of septa were significantly higher in the control group compared to the CLP group. Inferior origin was significantly more prevalent in the control group than in the CLP group ( P = .004). Conclusion There was no difference in the prevalence of MSS between patients with CLP and non-CLP individuals. However, certain features of the septa were different in patients with CLP.
Disruption of stem cell niche–confined R-spondin 3 expression leads to impaired hematopoiesis
Authors: Kurtova Antonina V. , Heinlein Melanie , Haas Simon , Velten Lars , Dijkgraaf Gerrit J. P. , Storm Elaine E. , Kljavin Noelyn M. , Boumahdi Soufiane , Himmels Patricia , Herault Aurelie , Mancini Andrew , Koeppen Hartmut , Dail Monique , Yan Qingxiang , Zhang Jianhuan , Koch Ute , Radtke Freddy , Modrusan Zora , Metcalfe Ciara , Piskol Robert , de Sauvage Frederic J. 
Published: August 2022

Abstract
Self-renewal and differentiation of stem and progenitor cells are tightly regulated to ensure tissue homeostasis. This regulation is enabled both remotely by systemic circulating cues, such as cytokines and hormones, and locally by various niche-confined factors. R-spondin 3 (RSPO3) is one of the most potent enhancers of Wnt signaling, and its expression is usually restricted to the stem cell niche where it provides localized enhancement of Wnt signaling to regulate stem cell expansion and differentiation. Disruption of this niche-confined expression can disturb proper tissue organization and lead to cancers. Here, we investigate the consequences of disrupting the niche-restricted expression of RSPO3 in various tissues, including the hematopoietic system. We show that normal Rspo3 expression is confined to the perivascular niche in the bone marrow. Induction of increased systemic levels of circulating RSPO3 outside of the niche results in prominent loss of early B-cell progenitors and anemia but surprisingly has no effect on hematopoietic stem cells. Using molecular, pharmacologic, and genetic approaches, we show that these RSPO3-induced hematopoietic phenotypes are Wnt and RSPO3 dependent and mediated through noncanonical Wnt signaling. Our study highlights a distinct role for a Wnt/RSPO3 signaling axis in the regulation of hematopoiesis, as well as possible challenges related to therapeutic use of RSPOs for regenerative medicine.
Evaluation of clonal hematopoiesis in pediatric ADA-SCID gene therapy participants
Authors: White Shanna L. , Lee Thomas D. , Toy Traci , Carroll Judith E. , Polsky Lilian , Campo Fernandez Beatriz , Davila Alejandra , Kohn Donald B. , Chang Vivian Y. 
Published: August 2022

Abstract
Autologous stem cell transplant with gene therapy (ASCT-GT) provides curative therapy while reducing pretransplant immune-suppressive conditioning and eliminating posttransplant immune suppression. Clonal hematopoiesis of indeterminate potential (CHIP)–associated mutations increase and telomere lengths (TLs) shorten with natural aging and DNA damaging processes. It is possible that, if CHIP is present before ASCT-GT or mutagenesis occurs after busulfan exposure, the hematopoietic stem cells carrying these somatic variants may survive the conditioning chemotherapy and have a selective reconstitution advantage, increasing the risk of hematologic malignancy and overall mortality. Seventy-four peripheral blood samples (ranging from baseline to 120 months after ASCT-GT) from 10 pediatric participants who underwent ASCT-GT for adenosine deaminase–deficient severe combined immune deficiency (ADA-SCID) after reduced-intensity conditioning with busulfan and 16 healthy controls were analyzed for TL and CHIP. One participant had a significant decrease in TL. There were no CHIP-associated mutations identified by the next-generation sequencing in any of the ADA-SCID participants. This suggests that further studies are needed to determine the utility of germline analyses in revealing the underlying genetic risk of malignancy in participants who undergo gene therapy. Although these results are promising, larger scale studies are needed to corroborate the effect of ASCT-GT on TL and CHIP. This trial was registered at www.clinicaltrials.gov as #NCT00794508.
Effects of Covid-19 vaccination on different semen parameters
Authors: Abd Ziad H. , Muter Samir A. , Saeed Ruya Abdulhadi M. , Ammar Omar 
Published: August 2022

Abstract
Background
The possible effects of COVID-19 vaccines on reproductive health and male fertility in particular have been discussed intensely by the scientific community and the public since their introduction during the pandemic. On news outlets and social media platforms, many claims have been raised regarding the deleterious effects of COVID-19 vaccines on sperm quality without scientific evidence. In response to this emerging conflict, we designed this study to evaluate and assess the effect of the Pfizer-BioNTech mRNA COVID-19 vaccine on male fertility represented by the semen analysis parameters.

Results
Comparing the semen parameters of the participants before and after vaccination, no statistically significant effects on semen volume, pH or normal sperm concentration and morphology were shown. However, there were statistically significant differences on total sperm motility (P = 0.05) and progressive motility (P = 0.02). These differences are clinically insignificant given the fact that both readings before and after vaccination were within the normal ranges, according to the WHO manual guidelines for the examination and processing of human semen.

Conclusion
Our data suggest that the Pfizer-BioNTech mRNA COVID-19 vaccine has no deleterious effects on semen parameters.
Accuracy of non-invasive methods for assessing the progress of labor in the first stage: a systematic review and meta-analysis
Authors: Pan Wan-Lin , Chen Li-Li , Gau Meei-Ling 
Published: August 2022

Abstract
Background
The World Health Organization in recent years has emphasized reducing the possibility of unnecessary interventions in natural childbirth, but little is known about the accuracy of non-invasive methods when assessing the progress of labor. This paper presents a literature review to assess strategies that support non-invasive methods for labor during the first stage. It evaluates the available evidence to provide the most suitable assessments and predictions that objectively identify the progress of low-risk labor during the first stage of labor.

Methods
A search for relevant literature was conducted using the electronic databases of PubMed, CINAHL, Web of Sciences, the Cochrane Library, Scopus, Medline (OVID), and CEPS, with publications up to November 2021. Records were screened against pre-specified inclusion/exclusion criteria and the potential papers from Google Scholar were examined to identify additional papers that may have been missed. The Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool was used to appraise the methodological quality of the included studies. The certainty of the evidence was assessed using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach. Two independent investigators extracted the review’s characteristics, and discrepancies were resolved by consensus. This review calculated individual and pooled sensitivity, specificity, and positive predictive values, which were exported to STATA (version 14; Stata Corp., College Station, TX) to represent the performance of diagnostic testing.

Results
Our search returned 2283 reports of which 13 fulfilled the inclusion criteria, accounting for 2594 women. The subjects were divided into groups according to the diagnostic tests used to assess the progress of their labor, including appearance assessment and sonographic imaging parameters (head perineum distance, HPD; angle of progression, AOP, and other parameters). HPD pooled sensitivity was 0.74 (0.65–0.82), and specificity was 0.77 (0.69–0.84). The pooled diagnostic odds ratio (DOR) was 8.21 (4.67–14.41) and 10.34 (5.02–21.27), respectively. The results of subgroup analysis showed that the summary sensitivity and specificity were of medium accuracy overall. The quality of evidence as assessed with GRADE was low.

Conclusion
Vaginal examination is an intrinsic element in the use of the partogram, while transperineal ultrasound can also be used as an auxiliary tool. However, the presence of publication bias within the parameters of ultrasound indicates that the diagnostic performance may be overestimated. Thus, randomized controlled trials or large-scale prospective cohort studies are necessary.
Effects of pulmonary fissure completeness on major outcomes in children after video-assisted thoracoscopic congenital lung malformation lobectomy
Authors: Huang Jin-Xi , Chen Qiang , Hong Song-Ming , Hong Jun-Jie , Cao Hua 
Published: August 2022

AbstractWe performed a single-centre retrospective analysis using data from databases that were prospectively maintained in our centre between January 2019 and September 2021. Patients were divided into two groups based on the degree of pulmonary fissure completeness (PFC), using the fissure development scoring system. Patients with grades 2 or 3 PFC were considered to have incomplete pulmonary fissures and were included in Group A, and patients with grades 0 and 1 were considered to have complete pulmonary fissures and were included in Group B. The differences in demographics, perioperative characteristics and clinic outcomes between the two groups were evaluated. Multivariate logistic regression analysis was performed. A total of 213 patients with congenital lung malformation (CLM) underwent video-assisted thoracoscopic lobectomy. There were 30 patients in Group A and 183 patients in Group B. Our data showed that compared with Group B, Group A had a higher incidence of complications, especially Clavien-Dindo grade II and grade III complications. The degree of PFC was significantly correlated with the length of chest tube drainage and postoperative hospital stay. Multivariate logistic regression analysis showed that the degree of PFC could be used to predict the incidence of postoperative complications.ConclusionsThe degree of PFC is a predictor of the incidence of complications after thoracoscopic lobectomy in children with CLM.
Mucocele: a rare complication following stapled haemorrhoidopexy
Authors: Wan Xing-Yang , Fu Yuan-Ji , Li Gui-Ming , Xiao Guo-Zhong , Guo Zhi-Wei , Ren Dong-Lin , Cao Bo , Lin Hong-Cheng 
Published: August 2022

Abstract
Background
Stapled haemorrhoidopexy (SH) has resulted in a unique collection of procedural complications with postoperative mucocele a particularly rare example. This study is designed to comprehensively describe the characteristics of rectal mucocele and discuss its pathogenesis following SH surgery.

Methods
A database of patients presenting with a rectal mucocele following an SH procedure was established and studied retrospectively.

Results
Seven patients (5 males; median age 32 years, range 20–75 years) were identified. All patients complained of variable anal discomfort with 5/7 presenting with inconstant anal pain, 2 with de novo evacuatory difficulty. These cases appeared at a median time of 6 months (range 2–84 months) after SH surgery.

Conclusion
Rectal Mucocele develops when mucosal fragments become embedded and isolated under the mucosa. It is a preventable complication of SH surgery by ensuring correct purse string placement prior to stapled haemorrhoid excision.