bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elevated blood pressure in youth in pediatric weight management programs in the Pediatric Obesity Weight Evaluation Registry (POWER)
Authors: Binns Helen J. , Joseph Madeline , Ariza Adolfo J. , Cuda Suzanne E. , Skinner Asheley C. , Xu Haolin , Tucker Jared M. , Hampl Sarah E. , Santos Melissa , Mayo Shawyntee , King Eileen C. , Kirk Shelley ,   the POWER Work Group
Published: January 2022

AbstractBlood pressure (BP) assessment and management are important aspects of care for youth with obesity. This study evaluates data of youth with obesity seeking care at 35 pediatric weight management (PWM) programs enrolled in the Pediatric Obesity Weight Evaluation Registry (POWER). Data obtained at a first clinical visit for youth aged 3–17 years were evaluated to: (1) assess prevalence of BP above the normal range (high BP); and (2) identify characteristics associated with having high BP status. Weight status was evaluated using percentage of the 95th percentile for body mass index (%BMIp95); %BMIp95 was used to group youth by obesity class (class 1, 100% to < 120% %BMIp95; class 2, 120% to < 140% %BMIp95; class 3, ≥140% %BMIp95; class 2 and class 3 are considered severe obesity). Logistic regression evaluated associations with high BP. Data of 7943 patients were analyzed. Patients were: mean 11.7 (SD 3.3) years; 54% female; 19% Black non‐Hispanic, 32% Hispanic, 39% White non‐Hispanic; mean %BMIp95 137% (SD 25). Overall, 48.9% had high BP at the baseline visit, including 60.0% of youth with class 3 obesity, 45.9% with class 2 obesity, and 37.7% with class 1 obesity. Having high BP was positively associated with severe obesity, older age (15–17 years), and being male. Nearly half of treatment‐seeking youth with obesity presented for PWM care with high BP making assessment and management of BP a key area of focus for PWM programs.
Adenosine/adenosine type 1 receptor signaling pathway did not play dominant roles on the influence of sodium–glucose cotransporter 2 inhibitor in the kidney of bovine serum albumin‐overloaded streptozotocin‐induced diabetic mice
Authors: Shimada Keiji , Kanasaki Keizo , Kato Makoto , Ogura Yoshio , Takagaki Yuta , Monno Itaru , Hirai Taro , Kitada Munehiro , Koya Daisuke 
Published: January 2022

AbstractAims/IntroductionSodium–glucose cotransporter 2 inhibitors (SGLT2i) have been shown to display excellent renoprotective effects in diabetic kidney disease with macroalbuminuria/proteinuria. Regarding the renoprotective mechanism of SGLT2i, a sophisticated hypothesis was made by explaining the suppression of glomerular hypertension/hyperfiltration through the adenosine/adenosine type 1 receptor (A1R) signaling‐mediated restoration of the tubuloglomerular feedback mechanism; however, how such A1R signaling is relevant for renoprotection by SGLT2i in diabetic kidney disease with proteinuria has not been elucidated.Materials and MethodsStreptozotocin‐induced diabetic CD‐1 mice were injected with bovine serum albumin (BSA) and treated with SGLT2i in the presence/absence of A1R inhibitor administration.ResultsWe found that the influences of SGLT2i are essentially independent of the activation of A1R signaling in the kidney of BSA‐overloaded streptozotocin‐induced diabetic mice. BSA‐overloaded diabetic mice showed the trend of kidney damage with higher glomerular filtration rate (GFR) and the significant induction of fibrogenic genes, such as transforming growth factor‐β2 and collagen type III. SGLT2i TA‐1887 suppressed diabetes‐induced GFR in BSA‐overloaded diabetic mice was associated with the significant suppression of transforming growth factor‐β2 and collagen type III; A1R‐specific inhibitor 8‐cyclopentyl‐1,3‐dipropylxanthine did not cancel the effects of TA‐1887 on either GFR or associated gene levels. Both TA‐1887 and 8‐cyclopentyl‐1,3‐dipropylxanthine‐treated BSA‐overloaded diabetic mice showed suppressed glycated hemoglobin levels associated with the increased food intake. When analyzing the association among histological evaluation, GFR and potential fibrogenic gene levels, each group of mice showed distinct correlation patterns.ConclusionsA1R signaling activation was not the dominant mechanism on the influence of SGLT2i in the kidney of BSA‐overloaded diabetic mice.
Identification of three clinical neurofibromatosis 1 subtypes: Latent class analysis of a series of 1351 patients
Authors: Bergqvist C. , Fertitta L. , Ezzedine K. , Jannic A. , Zehou O. , Ferkal S. , Combemale P. , Barbarot S. , Mazereeuw‐Hautier J. , Sbidian E. , Wolkenstein P. ,   NF France
Published: January 2022

AbstractBackgroundNeurofibromatosis 1 (NF1) is one of the most common inherited disorders characterized by mutations in the tumour suppressor gene NF1. Its clinical manifestations are highly variable and unpredictable. A specific NF1 mutation does not predict the severity or complications of the disease.ObjectiveThe objective of this study was to build an empirical classification scheme without any a priori hypotheses to identify the underlying NF1 subtypes that best explain the observed heterogeneity.MethodsWe performed latent class analysis (LCA) of 1351 consecutive NF1 patients aged >17 years seen between 2002 and 2014. Data and phenotypic features were collected prospectively on a standardized form.ResultsThe median age was 36.8 (17–81) years. A three‐class model showed the best fit: 706 (52%) belonged to the LC1 ‘Cutaneous neurofibromas’ class having preferentially cutaneous neurofibromas (99%), plexiform neurofibromas (63%) and blue‐red macules (29%); 593 (44%) belonged to the LC2 ‘Subcutaneous neurofibromas’ class characterized by the presence of at least 10 subcutaneous neurofibromas (21%) and a familial form (77%) and 52 (4%) belonged to the LC3 ‘Dysmorphic phenotype’ class characterized by dysmorphic features (78%) and learning difficulties (87%). Patients in LC1 had a higher likelihood of developing scoliosis (RR = 1.7, 95% confidence interval (CI) [1.2–2.4]). Patients in LC2 were more likely to be men (RR = 1.4, 95% CI [1.1–1.7]). Patients in LC3 were at higher risk of having an optic pathway glioma (RR = 4.8, 95% CI [1.9–11.8]) and epilepsy (RR = 4.5, 95% CI [1.8–11.6]).ConclusionOur findings invite the performance of a larger cohort study to test whether the various latent classes reflect different underlying genetic modifiers of these phenotypic traits.
Ridge gourd volatiles are attractive to gravid female melon fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae)
Authors: Shivaramu Subhash , Parepally Saravan Kumar , Chakravarthy Akshay Kumar , Pagadala Damodaram Kamala Jayanthi , Kempraj Vivek 
Published: January 2022

AbstractThe melon fruit fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae), is an important pest of cucurbits throughout India, South‐East Asia and Pacific islands. Attractants of female melon fly are of relevance as they could enhance the efficacy of current pest management practices. Previous studies have revealed several host fruits to be attractive to gravid female melon flies. However, studies on the chemical compounds responsible for their attraction are meagre. As an initial part of our study, we evaluated the response of gravid female melon flies to headspace volatiles of four different cucurbit fruits, namely bitter gourd, cucumber, ridge gourd and snake gourd. Electrophysiological and multiple‐choice olfactometer assays revealed that gravid melon flies significantly preferred ridge gourd volatiles over volatiles of other cucurbit fruits. Coupled gas chromatography‐electroantennogram detection (GC‐EAG) analysis of headspace volatiles of ridge gourd to gravid melon flies identified six EAG‐active compounds, namely α‐pinene, 1‐octen‐3‐ol, p‐cymene, p‐ethyl‐benzaldehyde, methyl salicylate and p‐cymen‐7‐ol. Individual EAG‐active compounds, except α‐pinene, were significantly attractive to gravid female flies. Moreover, a synthetic blend with the concentration and ratio of compounds as in the ridge gourd headspace samples was attractive to flies. Though, in a dual‐choice test, the preference of gravid melon flies to a synthetic blend was not significantly different from preference to ridge gourd volatile samples. Taken together, our results show that the synthetic blend of ridge gourd volatiles may be used to develop a potential lure for female melon fly, Z. cucurbitae.
Molecular analyses in wheat and Aegilops tauschii reveal a new orthologue of the leaf rust resistance gene Lr19 on chromosome 7DL of Ae. tauschii
Authors: Pourkhorshid Zahra , Dadkhodaie Ali , Shamloo‐Dashtpagerdi Roohollah 
Published: January 2022

AbstractLeaf rust is one of the most devastating wheat diseases worldwide, to which many resistance genes have been successfully introgressed from wheat wild relatives. Though the Thinopyrum ponticum‐derived leaf rust resistance gene Lr19 is widely effective, and previous studies have shown its likely presence in Aegilops tauschii, no thorough investigation has been conducted to confirm this. The current study examined the presence of Lr19 in Ae. tauschii using molecular and bioinformatic analyses. Accordingly, the wheat cultivar Thatcher was used as a susceptible control, and the Lr19‐carrying genotypes; Thatcher + Lr19 (TcLr19) and Agatha were used as resistant controls. Phenotyping using pathotype CDHLQ accompanied with genotyping using two DNA markers verified previous results of the likely presence of an orthologue for Lr19 on chromosome 7DL of Ae. tauschii (AtLr19). Sequencing of the STS GB marker products from Ae. tauschii and TcLr19 showed 99% homology between the corresponding fragments, confirming the phenotyping and genotyping results. Both sequenced fragments matched to the melatonin biosynthesis gene, O‐methyltransferase‐2 (OMT2). A hierarchical gene network was reconstructed using all identified putative genes within a genomic region containing 2.5 cM upstream and downstream of OMT2. Results indicated that numerous important biotic stress‐responsive genes such as RPM1, RGA2, TRIUR3, BURP12 and myosin‐11, were located downstream of melatonin as a master regulator molecule through the OMT2 node. To our knowledge, this is the first report of the identification of an orthologue for Lr19 in Ae. tauschii, which provides insights into the possible regulatory route of this gene.
How social rejection expectations and depressive symptoms bi‐directionally predict each other – A cross‐lagged panel analysis
Authors: Kirchner Lukas , Schummer Steffen Erik , Krug Henning , Kube Tobias , Rief Winfried 
Published: January 2022

AbstractBackgroundAlthough research suggests that social rejection expectations play a crucial role in the development and maintenance of depressive symptoms, it is not clear whether such expectations are a risk factor for depression or rather a consequence thereof. The present study addressed this issue by investigating the time‐lagged bi‐directional effects of social rejection expectations and depressive symptoms.MethodsIn an online survey, participants (N = 347) completed measures of social rejection expectations, depressive symptoms, interpersonal competencies, and perceived social support at baseline and 2 months later. The relationships between the variables were examined using path models and cross‐lagged path analyses.ResultsCross‐lagged path analyses provided evidence for a substantial positive effect of social rejection expectations at baseline on depressive symptoms at follow‐up in addition to the reverse effect. A mediator analysis indicated that neither interpersonal competencies nor perceived social support mediated these bi‐directional effects.ConclusionThe current results demonstrate that social rejection expectations and depressive symptoms bi‐directionally predict each other. Thus, social rejection expectations appears to be both a risk factor for – and a symptom of – depression. In order to prevent a vicious circle of social rejection expectations and depressive symptoms, we recommend the early detection and treatment of social rejection expectations. Moreover, social rejection expectations should be specifically addressed in cognitive‐behavioural treatment of depression.
Should suppliers allow capacity transfers?
Authors: Khanjari Neda , Duenyas Izak , Iravani Seyed M.R. 
Published: January 2022

This research considers situations in which buyers pay to reserve their suppliers' capacity for future use. The study specifically explores whether suppliers should provide transfer rights, allowing buyers unable to use all of their reserved capacity to transfer the excess to another buyer, and whether they should charge a transfer fee. The study finds that, in most cases, the supplier maximizes financial outcomes when the buyer releasing the excess capacity keeps most of the retail‐level profit from the transfer and the supplier does not charge a transfer fee.
Key aspects of domestic cat spermatogenesis
Authors: Gobello Cristina 
Published: January 2022

AbstractIn mammals, spermatogenesis is a complex and cyclic process in which a spermatogonia turns into a highly differentiated cell: the spermatozoa. Spermatogenesis comprises proliferation of spermatogonia (spermatocytogenesis), meiosis of spermatocytes and finally differentiation of spermatids into spermatozoa (spermiogenesis). This review summarizes the current knowledge on domestic cat spermatogenesis including its physiology, development, efficiency and pathologies as well as their novel non‐invasive diagnostic methods. This information will provide a resource for further studies to achieve precise fundamental knowledge of key aspects that will facilitate breeding, management and contraception in this popular species.
Where and why do we lose women from the continuum of care in maternal health? A mixed‐methods study in Southern Benin
Authors: Gryseels Charlotte , Dossou Jean‐Paul , Vigan Armelle , Boyi Hounsou Christelle , Kanhonou Lydie , Benova Lenka , Delvaux Thérèse 
Published: January 2022

AbstractObjectiveContinuum of care (CoC) in maternal health is built on evidence suggesting that the integration of effective interventions across pregnancy, childbirth, and the postnatal period leads to better perinatal health outcomes. We explored gaps along the CoC in maternal health in Benin.MethodsA mixed‐methods study triangulating results from a qualitative study in southern Benin with a quantitative analysis of Benin Demographic and Health Survey (BDHS) data on the use of services along the CoC was conducted.ResultsBenin Demographic and Health Survey analysis showed that although 89% of women reported at least one antenatal care (ANC) visit, only half initiated ANC in the first trimester and completed 4 or more visits. 85% reported facility‐based childbirth and 69% a postnatal check within 48 h after childbirth. Our qualitative study confirms early initiation of ANC and the transition from facility‐based childbirth to postnatal care are important gaps along the CoC and reveals late arrival at health facility for childbirth as an additional gap. These gaps interact with spiritual and alternative care practices that aim to safeguard pregnancy and prevent complications. Structural factors related to poverty and disrespectful care in health facilities compounded to limit the utilisation of formal healthcare.ConclusionsThe combined use of BDHS and qualitative data contributed to highlighting critical gaps along the maternal CoC. A lack of integration of spiritual or alternative aspects of care into biomedical services, as well as structural factors, impeded access to healthcare in Benin.
The timing of gamma irradiation and its effect on cell‐free and microvesicle‐bound hemoglobin. The BEST collaborative study
Authors: William Nishaka , Bicahlo Beatriz , Hansen Adele , de Korte Dirk , Acker Jason P. ,   Biomedical Excellence for Safer Transfusion (BEST) Collaborative
Published: January 2022

AbstractBackgroundGamma irradiation of red cell concentrates (RCCs) is regularly used to prevent transfusion‐associated graft‐versus‐host disease (TA‐GvHD) in at‐risk patients. While studies have indicated that irradiated RCCs exhibit increased hemolysis, there have been no efforts to differentiate between free‐ and microvesicle (MV)‐bound hemoglobin (Hb). As an increase in the proportion of free‐Hb in irradiated RCCs could alter vascular function, we sought to characterize differences in the state of extracellular Hb based on the timing of irradiation.Study Design and MethodsFour separate pools of seven CPD/SAGM leukoreduced RCCs were produced and split into four sets of seven identical units. The units from each set were subject to irradiation (25 Gy) at six different points during storage, with one unit serving as a nonirradiated control. All testing was performed immediately following unit expiry on day 43.ResultsThe earlier in storage that units were irradiated, the higher the hemolysis and the lower the proportion of MV‐bound Hb. Units irradiated earlier in storage (1–8 days post collection) additionally had lower membrane rigidity (KEI), lower mean corpuscular Hb concentrations (MCHC), and higher mean corpuscular fragility (MCF). Morphology indices, mean cell volume (MCV), mean corpuscular Hb (MCH), phosphatidylserine (PS) expression, as well as MV production and size did not however differ significantly between groups based on the timing of irradiation.ConclusionsOur findings indicate that irradiation timing can alter the state of extracellular Hb, with “early” irradiation promoting an increased proportion of cell‐free Hb as well as mechanical damage to the RBC membrane.
The novel c.634+4A>G splicing variant inRHCEresults in weak C and e antigen expression in a pregnant woman originated from Japan
Authors: Restivo Cynthia , Le Bras Myriam , Deguigne Pierre‐Antoine , Le Glatin Laurence , Guerry Christine , Férec Claude , Le Maréchal Cédric , Beloeil Rémi , Fichou Yann 
Published: January 2022

AbstractBackgroundIn theRHblood group genes, molecular variants that alter antigen expression with potential clinical relevance are frequently identified and reported in the literature.Study Design and MethodsA pregnant woman in her first pregnancy, who originates from Japan, was typed by routine serological testing. TheRHCEgene was investigated to identify single nucleotide variants (SNVs) and/or structural variants by a commercial platform, Sanger sequencing, and quantitative multiplex PCR of short fluorescent fragments. The haplotypes were determined by sequencing PCR fragments generated from genomic DNA and subcloned into a plasmid vector. Effect on splicing was predicted by bioinformatics tools, including SpliceAI and the splicing module of Alamut. In parallel, functional analysis was carried out by a minigene splicing assay.ResultsA patient with no transfusion history was typed RH:1,2w,3,4,5w. An unreported single variant was identified inRHCEintron 4 at the heterozygous state: c.634+4A>G. Minigene splicing assay showed that this SNV decreases significantly the relative abundance of the full‐length transcript, in accordance with the predictions made by the Alamut tools, but not SpliceAI, suggesting expression of a normal RhCE protein.ConclusionOverall, the novelRHCE*02(c.634+4A>G)allele alters quantitatively, but not qualitatively, the expression of C and e in the RH blood group system, indicating that the patient is not at risk for alloimmunization and may safely receive C+e+ red blood cell units. This report illustrates the relevance of functional assays for the interpretation of rare variants and, specifically, how it may help guide transfusion management in patients.
The Pathomechanism of Spondylolytic Spondylolisthesis in Pediatric Lumbar Spines: In Vitro and Finite Element Assessments
Authors: Konz Regina J. , Goel Vijay K. , Grobler Leon J. , Grosland Nicole M. , Spratt Kevin F. , Scifert Jeffrey L. , Sairyo Koichi 
Published: January 2022

Abstract
Experimentally, it was determined that the weakest link for A-P shear force in the immature baboon lumbar spine with pars interarticularis defects was between the growth-plate and osseous end-plate, even with an injured disc. Immature baboon finite element models (FEM) as well as an immature human FEM were developed. Numerical results predicted that a thin pars will increase stresses at the pars, eventually leading to a pars defect. Furthermore, a fracture in the pars interarticularis results in substantially greater loads being carried by the growth-plates. Upon combination of the experimental and numerical aspects of this study, it is possible to predict the A-P shear force needed for spondylolisthesis to occur.
Measurement of Cartilage Fluid Pressurization in Confined Compression Cyclical Loading
Authors: Soltz Michael A. , Ateshian Gerard A. 
Published: January 2022

Abstract
In recent studies (Oloyede and Broom, 1991; Soltz and Ateshian, 1997, 1998) it has been demonstrated from experiment and theory that compressive stresses applied across the surface of articular cartilage are supported primarily by the hydrostatic pressurization of the interstitial fluid, under configurations of confined compression creep and stress-relaxation. This pressurization is sustained for several hundred seconds before it eventually subsides, suggesting a major role for interstitial fluid in protecting the solid collagen-protcoglycan matrix from excessive stresses. In the present study, cartilage fluid pressurization is investigated experimentally and theoretically under sinusoidal loading, since the mechanical environment of articular cartilage during daily living is often cyclical in nature. Investigators have demonstrated that this cyclical loading may cause a biosynthetic response of chondrocytes within the tissue, depending on the frequency and amplitude of the applied stresses or strains (e.g. Gray et al., 1988; Hall et al., 1991; Kim et al., 1995; Parkikinen et al., 1992; Sah et al., 1989). A theoretical solution for cyclical loading in confined compression has been obtained previously (Suh et al., 1995) using the biphasic theory (Mow et al., 1980) and this solution is employed here.
Effect of Pulsatile Flow on Monocytic Cell Adhesion to E-Selectin Coated Surfaces of a Three-Dimensional Perfusion Model
Authors: Hinds Monica Thacker , Jones Steven A. , Alevriadou B. Rita , Giddens Don P. 
Published: January 2022

Abstract
We report on effects of pulsatile flow on monocytic cell adhesion to the surface of a model in which flow characteristics thought to be important in atherogenesis are simulated. The surface was coated with E-selectin, and adhesion of activated monocytes was investigated under pulsatile flow conditions and compared with uncoated models and steady flow experiments. Coating the model with E-selectin increased the adhesion of cells in all regions in the vertical model. However, pulsatile flow with the same mean Reynolds number as for steady flow significantly reduced the number of adherent cells throughout the model.
Contraction Kinetics of Cell-Seeded Collagen Gels: Effects of Cell-Seeding Density
Authors: Awad Hani , Wu Ying , Butler David , Boivin Gregory 
Published: January 2022

Abstract
Advances in our knowledge and experience with mesenchymal stem cells (MSCs) and their potential use in repair and regeneration of skeletal tissue, such as tendon [1,7], cartilage [6] and bone [3], makes this multipotential cell an attractive choice for such transplantation therapies. Our search for appropriate cell-delivery scaffolds, however, continues. We have utilized collagen to construct cell-seeded implants for tendon repair because collagen is the most abundant protein in these tissues.