bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polyphenols Profile and Enzyme Inhibitory Properties of Blumea lacera (Burm. f.) DC.: A Potential Candidate against Obesity, Aging, and Skin Disorder
Authors: Swaraz A. M. , Sultana Fariha , Shahin Ahmed Khondoker , Satter Mohammed A. , Hossain Hemayet , Raihan Obayed , Brishti Afrina , Khalil Ibrahim , Hua Gan Siew 
Published: August 2022

AbstractBlumea lacera (Burm. f.) DC. is attracting scientific interest due to the diverse biological activities of its various parts and its use in folk medicine. The present study was undertaken to investigate the tissue‐specific differential expression pattern of its total bioactive compounds. The study was further extended to whole plant phenolics profiling, in vitro enzyme inhibition activities, followed by in silico enzyme inhibition analysis to assess its potential as herbal medicine. The amount of total phenolics in different tissues was followed in decreasing order as old leaf, flower bud, root, young leaf, flower, old stem, and young stem, while that for the flavonoids was old leaf, root, young leaf, flower bud, flower, young stem, and old stem. This study identified rosmarinic acid, quercetin, and kaempferol in this plant for the first time. The solvent extracts demonstrated strong inhibition of lipase and tyrosinase activity, along with varying degrees of inhibition of acetylcholinesterase and butyrylcholinesterase activity. Among the detected compounds, ten displayed strong in silico binding affinities with the tested enzymes. The findings provide a new insight into further investigation of the medicinal potential of this species against obesity, neurological disorders, and aberrant skin color.
Selective Acylation of Proteins at Gly and Lys in His Tags
Authors: Jensen Knud J. , Thygesen Mikkel B. , Sørensen Kasper K. , Wu Shunliang , Treiberg Tuule , Schoffelen Sanne 
Published: August 2022

AbstractThe chemical modification of proteins is of great importance in chemical biology, biotechnology, and for the production of modified biopharmaceuticals, as it enables introduction of fluorophores, biotin, half‐life extending moieties, and more. We have developed two methods that use poly‐His sequences to direct the highly selective acylation of proteins, either at the N‐terminus or at a specific Lys residue. For the former, we used an N‐terminal Gly‐His6 segment (Gly‐His tag) that directed acylation of the N‐terminal Nα‐amine with 4‐methoxyphenyl esters, resulting in stable conjugates. Next, we developed the peptide sequences Hisn‐Lys‐Hism (Lys‐His tags) that direct the acylation of the designated Lys Nϵ‐amine under mild conditions and with high selectivity over native Lys residues. Both the Gly‐His and Lys‐His tags maintain the capacity for immobilized metal ion affinity chromatography. We have demonstrated the robustness of these methods by attaching different moieties such as azides, fluorophores, and biotin to different proteins, including antibodies.
Developments in Downstream Butanol Separation from Acetone, Butanol, and Ethanol Fermentation
Authors: Gonçalves Bruno José Arcanjo , Figueiredo Kátia Cecília de Souza 
Published: August 2022

AbstractBiobutanol is known for its potential as a biofuel and for having various other industrial applications. However, technical and economic constraints still restrict its commercial application. Biobutanol is produced via acetone, butanol, and ethanol (ABE) fermentation, in which acetone and ethanol are also produced, mainly by Clostridium sp.; however, it is toxic to the cells. Efforts have been made to improve the process by separating butanol from the fermentation broth. This work presents an overview of the main processes reported in ABE separation in the literature, focusing on the pervaporation approach. Some of the latest developments in the field are stated, aiming at comparing the separation processes.
Palladium‐Catalyzed Enantioselective Cyclization of 1,6‐Enynes through Intramolecular Chlorine Transfer as a Novel Strategy for Asymmetric Halopalladation
Authors: Tang Junlong , Zhang Liren , Wu Wanqing , Yang Shaorong , Jiang Huanfeng 
Published: August 2022

AbstractPalladium‐catalyzed enantioselective cyclization of enynes has contributed significantly to the construction of chiral cyclic molecules. In contrast, the catalytic asymmetric cyclization involving halopalladation remains an unresolved challenge with the inevitable disturbance of the halide ions. Herein, an intramolecular chlorine transfer strategy is used to accomplish the enantioselective chloropalladation cyclization of 1,6‐enynes. This reaction provides a redox‐neutral approach to a variety of chiral α‐chloromethylene‐γ‐butyrolactones with excellent E selectivity and enantioselectivity. The precisely controlled coordination of palladium with both the in situ generated nucleophilic species and the monodentate phosphoramidite ligand is crucial for enantioselectivity.
Research on extraction of Cr(III) by D2EHPA/n‐octanol/sulphonated kerosene
Authors: He Lantao , Zhou Yujie , Zhu Zijun , Luo Jianhong 
Published: August 2022

AbstractChromium is an important industrial raw material. As far as China is concerned, chromium is in great demand, which is dependent on imports and large emissions. So recovery of chromium has important economic and environmental protection value. The extraction and back extraction of Cr(III) from solution by the extractant diisooctyl phosphate (di‐2‐ethylhexylphosphoric acid [D2EHPA]) with n‐octanol as assistant and sulphonated kerosene as diluent was studied. The effects of saponification rate, phase ratio, temperature, condensation of extract, aging of extractant, and pH of aqueous phase on extraction equilibrium were discussed, and the formulation of extractant was optimized. The coordination number of the extraction reaction under specific conditions was discussed by the saturation capacity method. The extraction reaction kinetics were mathematically characterized by binary linear regression. The advantages and disadvantages of back extraction with H2SO4 or NaOH were compared. At last, a process to realize the recycling of extractant was obtained.
Noninvasive computed tomography derived fractional flow reserve simulation based on microvascular tree model reconstruction
Authors: Yu Long , He Wei , Qin Wang , Wang Keqiang , Guo Weifeng , Wang Shengzhang 
Published: August 2022

AbstractTo establish a novel method for noninvasive computed tomography derived fractional flow reserve (CT‐FFR) simulation based on microvascular tree model reconstruction and to evaluate the feasibility and diagnostic performance of the novel method in coronary artery disease compared with invasive fractional flow reserve (FFR). Twenty patients (20 vessels) who underwent coronary computed tomography angiography (CCTA) and invasive FFR were retrospectively studied. The anatomic epicardial coronary artery model was reconstructed based on CCTA image, and the microvascular tree model was simulated based on patient‐specific anatomical structures and physiological principles. Numerical simulation was subsequently performed using the CFD method with full consideration of the variation of viscosity in microvascular. Two patients with the FFR value of .80 were selected for adjusting the parameters of the model, while the remaining 18 patients were selected as a validation cohort. After simulation, CT‐FFR was compared with invasive FFR with a threshold of .80. Eleven (55%) patients had an abnormal FFR that was less than or equal to .80. Compared with invasive FFR, sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of CT‐FFR with an optimal threshold of .80 were 100%, 77.8%, 81.8%, 100%, 88.89%, respectively. There were a good correlation and consistency between CT‐FFR and invasive FFR. Time per patient of CT‐FFR analysis was less than 15 min. CT‐FFR based on microvascular tree model reconstruction is feasible with good diagnostic performance. It requires a short processing time with excellent accuracy. Large multicenter prospective studies are required for further demonstrating the diagnostic performance of this novel model in myocardium ischemia evaluation.
Tunable Naphthalimide/Cinnoline‐Fused (CinNapht) Hybrid Dyes for Fluorescence Imaging in Living Cells
Authors: Hoang Minh‐Duc , Savina Farah , Durand Philippe , Méallet‐Renault Pr. Rachel , Clavier Gilles , Chevalier Arnaud 
Published: August 2022

AbstractThis paper presents the synthesis, photophysical characterization and use for cell imaging of 14 fluorophores derived from a hybrid Naphthalimide/Cinnoline fused backbone called “CinNapht”. Photophysical properties of these fluorophores including absorbance, excitation and emission spectra have been recorded in CHCl3, DMSO and PBS+5 %BSA. They exhibit red emission associated to a large Stokes shift and fluorescence quantum yields up to 52 %. Theoretical calculations have been undertaken in order to provide elements of rationalization for a major part of the results. Some of these analogues have been tuned to enable imaging of cell organelles such as mitochondria, lysosome or lipid droplets. This study comes to confirm that CinNapht dyes can be considered as relevant alternatives to existing tools for cell imaging.
Manipulating adrenergic stress receptor signaling to enhance immunosuppression and prolong survival of vascularized composite tissue transplants
Authors: Kim Minhyung , Fisher Daniel T. , Bogner Paul N. , Sharma Umesh , Yu Han , Skitzki Joseph J. , Repasky Elizabeth A. 
Published: August 2022

AbstractBackgroundVascularized composite tissue allotransplantation (VCA) to replace limbs or faces damaged beyond repair is now possible. The resulting clear benefit to quality of life is a compelling reason to attempt this complex procedure. Unfortunately, the high doses of immunosuppressive drugs required to protect this type of allograft result in significant morbidity and mortality giving rise to ethical concerns about performing this surgery in patients with non‐life‐threatening conditions. Here we tested whether we could suppress anti‐graft immune activity by using a safe β2‐adrenergic receptor (AR) agonist, terbutaline, to mimic the natural immune suppression generated by nervous system‐induced signalling through AR.MethodsA heterotopic hind limb transplantation model was used with C57BL/6 (H‐2b) as recipients and BALB/c (H‐2d) mice as donors. To test the modulation of the immune response, graft survival was investigated after daily intraperitoneal injection of β2‐AR agonist with and without tacrolimus. Analyses of immune compositions and quantification of pro‐inflammatory cytokines were performed to gauge functional immunomodulation. The contributions to allograft survival of β2‐AR signalling in donor and recipient tissue were investigated with β2‐AR−/− strains.ResultsTreatment with the β2‐AR agonist delayed VCA rejection, even with a subtherapeutic dose of tacrolimus. β2‐AR agonist decreased T‐cell infiltration into the transplanted grafts and decreased memory T‐cell populations in recipient's circulation. In addition, decreased levels of inflammatory cytokines (IFN‐γ, IL‐6, TNF‐α, CXCL‐1/10 and CCL3/4/5/7) were detected following β2‐AR agonist treatment, and there was a decreased expression of ICAM‐1 and vascular cell adhesion molecule‐1 in donor stromal cells.Conclusionsβ2‐AR agonist can be used safely to mimic the natural suppression of immune responses, which occurs during adrenergic stress‐signalling and thereby can be used in combination regimens to reduce the dose needed of toxic immunosuppressive drugs such as tacrolimus. This strategy can be further evaluated for feasibility in the clinic.
Daprodustat for anaemia in patients with heart failure and chronic kidney disease: A randomized controlled study
Authors: Iso Takashi , Matsue Yuya , Mizukami Akira , Tokano Takashi , Isoda Kikuo , Suwa Satoru , Miyauchi Katsumi , Yanagisawa Naotake , Okumura Yasuo , Minamino Tohru 
Published: August 2022

AbstractAimsHypoxia‐inducible factor‐prolyl hydroxylase (HIF‐PH) inhibitors have been developed for the treatment of renal anaemia; however, no study has evaluated the safety and efficacy of HIF‐PH inhibitors in patients with heart failure (HF). This study was designed to evaluate the safety and efficacy of daprodustat, a HIF‐PH inhibitor, in patients with HF and renal anaemia.Methods and resultsWe designed a pilot, multi‐centre, open‐label, randomized controlled study, in which 50 patients with HF complicated with chronic kidney disease and anaemia will be randomized 1:1 to either the daprodustat or control group at seven sites in Japan. Study entry requires New York Heart Association Class II HF symptoms or a history of hospitalization due to HF, an estimated glomerular filtration rate of <60 mL/min/1.73 m2, and a haemoglobin level of 7.5 to <11.0 g/dl. Patients randomized to the daprodustat group will be treated with oral daprodustat, and the dose will be uptitrated according to the changes in the haemoglobin level from previous visits. In this study, we will evaluate the impact of HIF‐PH inhibitors on cardiac function using advanced cardiovascular imaging modalities, including cardiac magnetic resonance imaging. The primary outcome is the haemoglobin level at 16 weeks of randomization, and all adverse events will be recorded and evaluated for any association with daprodustat treatment.ConclusionConsidering the hypothetical upside and downside of using HIF‐PH inhibitors in anaemic patients with HF and chronic kidney disease, and because there are virtually no safe and effective treatments for patients with anaemia not caused by iron deficiency, our study results will contribute significantly to this field.
Solution Structure and Relaxivity of Ln‐DOTXAZA Derivatives
Authors: Holzapfel Malte , Baldau Torben , Kerpa Svenja , Guadalupi Gianmarco , Qi Bing , Liu Yang , Parak Wolfgang J. , Maison Wolfgang 
Published: August 2022

AbstractHerein we report the synthesis and structural analysis of tunable 1,4,7,10‐tetraazacyclododecane‐1,4,7,10‐tetraacetic acid (DOTA)‐derivatives bearing azidoethyl side chains. According to the number of azidoethyl side chains, the compounds are termed DOTXAZA (with X=1–4). All derivatives retain the well‐approved DOTA scaffold for metal complexation with 8 coordinating functionalities and are applicable to Cu‐catalyzed alkyne‐azide cycloaddition (CuAAC). They may thus be used to assemble new Gd‐based contrast agents (GBCAs) with tailored pharmacokinetic or MRI properties retaining the high stability of clinically used DOTA‐derived GBCAs. The complex geometry of all DOTXAZA derivatives was investigated in solution with NMR‐spectroscopy of the corresponding Eu‐complexes. A correlation of complex geometry with the longitudinal relaxivity of corresponding Gd‐complexes revealed an increase in relaxivity with increasing substitution number in the DOTXAZA series. Moreover, CuAAC‐modified DOTAZA‐derivatives with neutral, charged, and zwitterionic groups were prepared. Although of similar molecular mass, the corresponding Gd‐complexes revealed pronounced differences in relaxivities from r1=3.9–7.9 mm−1 s−1 in water at 1.5 T and 37 °C. Relaxivities of these complexes were found to be strongly dependent on side chain charge and are most likely determined by second‐sphere water effects.
Reduced‐order models for the seismic assessment of plan‐irregular low‐rise frame buildings
Authors: Ruggieri Sergio , Chatzidaki Akrivi , Vamvatsikos Dimitrios , Uva Giuseppina 
Published: August 2022

AbstractA procedure is presented for deriving low‐complexity structural models to predict the global response of asymmetric‐plan low‐rise frame buildings for purposes of class‐level assessment. As a compromise between employing a full‐scale multi‐degree‐of‐freedom structural model versus an equivalent single‐degree‐of‐freedom one, the challenge is to create an idealized 3D structure with few degrees‐of‐freedom that can match the inelastic response of a building for which full knowledge of geometrical and mechanical properties is available. Such a 3D reduced‐order model can offset the computational cost related to performing multiple nonlinear dynamic analyses within the framework of Performance‐Based Earthquake Engineering. To this goal, rules and equations are proposed for achieving equivalence among the linear and nonlinear properties (e.g. mass, stiffness, strength) of the building analysed and the related 3D reduced‐order model. The procedure is applied on a sample of 15 existing reinforced‐concrete frame school buildings, from the province of Foggia in Southern Italy, for which the full numerical models are available. Both calibrated and uncalibrated reduced‐order models are created, exploring the limitations of the proposed order‐reduction in a real‐life case study.
A critical review on the practical use of lithium cycle as an upfront nitrogen removal technology from natural gas
Authors: Omar Abdullah , Ibrahim Shadwa , Almomani Fares 
Published: August 2022

AbstractThe increase in energy demand as the world population grows, as well as the competition in the liquefied natural gas (LNG) market, force producers to work hard on developing cost‐effective production technologies. Upfront nitrogen removal (UNR) before the LNG plant's cold section is considered a promising option to save energy that would otherwise be wasted to cool down a large volume of unused nitrogen in the gas stream. In this study, the use of the lithium cycle (Li‐Cy) as a cost‐effective method for UNR is investigated. The Li‐Cy is compromised of three stages: lithium chemisorption of nitrogen (), hydrolysis of lithium nitride (), and electrowinning (Elec.‐w) of the final product to precipitate lithium metal for further reuse. The relevant chemistry, applicability, economic, and future challenges of Li‐Cy as a UNR technology from natural gas (NG) were explored and discussed. The main challenges that required further investigation to apply Li‐Cy to large‐scale applications were highlighted for future works. The literature review revealed that Li‐Cy can spontaneously remove nitrogen from NG even at low temperatures and produces ammonia as a valuable hydrogen storage material. The used lithium can be regenerated via and Elec.‐w and reused again many times. The cost of the Li‐Cy can be compensated by energy savings, the increase in production rate, and by selling the generated ammonia. Calculations showed that selling the produced ammonia from LNG plants with capacity in the range of 1–5 MTPA would not only offset the costs of Li‐Cy but would generate a net profit of $21MM to $103MM, respectively.
The superefficiency direction distance function and total factor energy efficiency: Evidence from the comprehensive and progressive agreement for trans‐pacific partnership (CPTPP) member countries
Authors: Feng Ying , Lu Ching‐Cheng , Lee An‐Guey , Tang Pao‐Yu , Yang An‐Chi , Zhang Leibao 
Published: August 2022

AbstractThis study used the superefficiency direction distance function and total factor energy efficiency to evaluate the changes in energy efficiency and total factor energy efficiency of 11 member countries of the Comprehensive and Progressive Agreement for Trans‐Pacific Partnership (CPTPP) from 2013 to 2017. Based on the results, we consider the policy implications for CPTPP members, and suggest approaches to improve the direction and range of the difference variable for aalyzing energy efficiency performance. In the selection of variables, input variables include labor force, energy consumption, and capital formation; undesirable output variables include the domestic production gross, life expectancy, and PM2.5. The results show that Canada, Japan, and Mexico have the best performance in terms of energy superefficiency and total factor energy efficiency; in contrast, Chile, Malaysia, Peru, and Vietnam would benefit from improved energy efficiency. Energy efficiency can be improved by reducing labor and final energy consumption, increasing gross domestic product, and reducing PM2.5. However, simultaneously improving all three factors is challenging. Countries should first focus on appropriately adjusting energy policies, actively developing new energy use, improving production technology, avoiding waste, reducing PM2.5, and accelerating urban development to achieve optimal energy efficiency.
Congestion centric multi‐objective reptile search algorithm‐based clustering and routing in cognitive radio sensor network
Authors: Sunitha D. , Balmuri Kavitha Rani , de Prado Rocío Pérez , Divakarachari Parameshachari Bidare , Vijayarangan R. , Hemalatha K. L. 
Published: August 2022

AbstractIn recent trends, Cognitive Radio Sensor Networks (CRSNs) are investigated in‐depth and getting momentum in all types of applications. CRSN can make use of the underutilized frequency spectrum in a suitable manner. Due to the above‐mentioned advantage, the scholars have initiated to study of the domain of cognitive radio routing. Network congestion produces transmission delays and packet loss, as well as time and energy wasted on recovery. In order to fulfill the energy efficiency and network lifetime in CRSN, Congestion Centric Multi‐Objective Reptile Search Algorithm (CC‐MORSA)‐based Clustering and Routing are used. The main objective of proposed CC‐MORSA is to improve the lifetime by minimizing the distance among the designated Cluster Head nodes which creates the fitness function by multiple objectives like energy, distance, and load. This technique is appropriate for common sensor nodes in coordinated communications infrastructure and large networks. The simulation results are analyzed through MATALB in terms of remaining energy (999.5 J), average delay (0.36 s), Packet Delivery Ratio (99.8%), Energy Consumption (24.1 J), Throughput (0.98 Mbps), routing overhead (0.54), and Packet Loss Rate (0.2%). From the outcomes, it shows that the presented CC‐MORSA outperformed conventional Stability‐Aware Cluster‐based Routing and Drop Factor‐Based Energy Efficient Routing technique.
How the country context shapes firms' competitive repertoire complexity
Authors: Steinberg Philip J. , Hennig Jan C. , Oehmichen Jana , Heigermoser Judith 
Published: August 2022

AbstractResearch SummaryRecent research has shown that firms' ability to employ complex competitive repertoires can create long‐term competitive advantages. Since research on its determinants has focused on the firm level, we lack an understanding of how country‐level factors impact firms' implementation of complex competitive repertoires. Our cross‐country study addresses this gap by integrating a model of country‐level competitiveness factors with insights from the literature on competitive dynamics and portable governance. We argue that a country context with high‐quality competitiveness factors enables firms to implement complex competitive repertoires. In addition, we hypothesize that firms with foreign investors from countries with high‐quality competitiveness factors can partially compensate for low‐quality factors in firms' domestic context. We found support for our hypotheses in an unbalanced sample containing 1,340 firms from 32 countries.Managerial SummaryEmploying complex competitive repertoires (i.e., diverse and dynamic arrays of competitive actions), such as price reductions or new product introductions, can help firms outcompete their competition. We argue and empirically show that firms' domestic country context, specifically high‐quality governance, factor and demand conditions, related and supporting industries, and strong context for rivalry drive their ability to implement complex repertoires. Moreover, we find that ownership by foreign investors from favorable country backgrounds can partly compensate for firms' weak conditions at home by serving as enabling bridges. Managers who aim to improve their firms' repertoire complexity but are restricted by their domestic country context may consider attracting foreign investors from countries that have what their countries lack.