bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surgical and Functional Outcomes on Omitting Cortical Renorrhaphy During Robot-Assisted Versus Laparoscopic Partial Nephrectomy: A Match-Paired Analysis
Authors: Kubota Masashi , Yamasaki Toshinari , Murata Shiori , Abe Yohei , Tohi Yoichiro , Mine Yuta , Hagimoto Hiroki , Kokubun Hidetoshi , Suzuki Issei , Tsutsumi Naofumi , Inoue Koji , Kawakita Mutsushi 
Published: January 2022

Abstract
Objectives

To assess surgical and functional outcomes in comparison of cortical renorrhaphy omitting, robot-assisted partial nephrectomy (CRO-RAPN), and laparoscopic partial nephrectomy (CRO-LPN).
Methods

Between July 2012 and June 2020, patients with localized clinical T1-2 renal masses who underwent CRO-RAPN or CRO-LPN were reviewed. The outcomes of the CRO-RAPN and CRO-LPN groups were compared using propensity score matching. Trifecta was defined as negative surgical margin, less than 25 minutes of warm ischemic time, and no complications of Clavien-Dindo grade III or more until three months postoperatively. Preservation rate of the estimated glomerular filtration rate (eGFR) was evaluated at six months postoperatively.
Results

A total of 291 patients, including 210 patients who underwent CRO-RAPN and 81 patients who underwent CRO-LPN, were included, and matched pairs of 150 patients were analyzed. The CRO-RAPN group was associated with a significantly shorter warm ischemic time (13 min vs 20 min, P < 0.001), shorter total operation time (162 min vs 212 min, P < 0.001), less estimated blood loss (40 mL vs 119 mL, P = 0.002), lower incidence of overall complications (3% vs 16%, P = 0.001), higher preservation rate of eGFR at six months postoperatively (93% vs 89%, P = 0.003), and higher trifecta achievement rate (84% vs 64%, P = 0.004) than the CRO-LPN group.
Conclusions

CRO-RAPN contributes to a shorter warm ischemic time, less blood loss, fewer complications, and preservation of renal function and makes it feasible to achieve a higher rate of trifecta compared to CRO-LPN.
Investigation on the Needs of Preoperative Health Education of Parents, Whose Children Undergoing Day Surgery
Authors: LIAO Rong-ji , ZHANG Mei-xue 
Published: January 2022

Abstract
BackgroundTo explore the needs of preoperative health education of parents，whose children undergoing day surgery, and to provide reference for nurses to carry out health education. MethodsThe parents whose children undergoing day surgery in Guangzhou Women and Children,s medical center from January 2021 to February 2021 were selected as the research objects. The needs of parents for preoperative health education were investigated by questionnaire, and the needs and satisfaction of parents for the timing, content, ways and push methods of preoperative health education were observed. Results591 questionnaires were distributed and 578 were recovered, with a recovery rate of 97.80%. The results showed that the parents had the highest demand for preoperative health education when they made an appointment for day surgery. The most desired content of health education was admission procedure, and the most desired way to receive health education was through video. The most acceptable push mode was through WeChat official account. Parents' satisfaction with the current health education was 94.64%. ConclusionsThe content of preoperative health education should be formulated according to the needs of parents, whose children undergoing day surgery. The time of providing health education should be moved forward. Health education videos should be given first, combined with a variety of health education methods, and health education videos should be pushed through WeChat official account or WeChat video number, so as to ensure the smooth development of children's day surgery and enhanced recovery after surgery.
Deconvolving The Effects of Coastal Erosion and Sea-Level Rise in Assessing Shoreline Retreat Along Semi-Arid Urban Coasts
Authors: Amrouni Oula , Heggy Essam , Hzami Abderraouf 
Published: January 2022

Abstract
The alarming vulnerability of low-lying sandy beaches to the acceleration of global sea level rise has been confirmed in the recent IPCC AR6 report. The situation is worsened by increasing coastal erosion, resulting in additional shoreline retreat of sandy beaches along several semi-arid urban coastal areas around the globe. The additional shoreline retreats from erosion are indicative of the rising imbalance in coastal sedimentary processes, which are a direct consequence of changes in precipitation patterns, urban growth, and change in land use. To quantify the magnitude and timescale of both coastal erosion and sea-level rise (SLR) in generating shoreline retreat of sandy beaches in semi-arid urban areas, we combine photogrammetric and statistical methods to measure and forecast the decadal evolution of these coastlines using two well-characterized sites that are hypothesized herein to be globally representative of these types of coasts undergoing rapid urban growth. We use multi-decadal shoreline positioning and land use classification surveys of the Southern California (SC, USA) and the Hammamet-North (HAM, Tunisia) beaches from aerial and orbital photogrammetric images, combined with the Digital Shoreline Analysis System, for the period from 1985 to 2018. Our results suggest that the current average shoreline retreat rates of sandy beaches range from -0.75 to -1.24 m/yr in SC and from -0.21 to -4.49 m/yr in HAM under similar aridity, land coverage and precipitation patterns. The observed decadal changes in shoreline positions along these semi-arid urban coastal areas are found to be accentuated by anthropogenic drivers associated with extensive urbanization, causing sediment imbalance at the coastline, adding up to the effect of the accelerating SLR. We assess that ~81% and 57% of the observed shoreline retreat was due to SLR, and 19% to 43% due to coastal erosion from urban growth along SC and HAM beaches, respectively. Using these measured rates, we establish a semi-empirical numerical model that combines urban growth and the observed shoreline retreat rate to forecast retreat rates through 2100 for both of our study areas, inferred herein to be representative of other global semi-arid urban coasts. Our model suggests that future average total shoreline retreat rates, accounting for both urban growth and SLR, range from -2 to -4 m/yr for SC and HAM sandy beaches, respectively, through 2100. The above suggests that if no mitigation is made, by 2100 the cumulative shoreline retreat in these urban areas could significantly exceed the Global Scale Assessment Model’s [46] cumulative projected average retreat of -30 m, confirming the alarming vulnerability of the semi-arid coastal urban areas that would need intensive and costly beach nourishment to control increasing shoreline erosion.
Rapid Differentiation From Human Induced Pluripotent Stem Cells Into Functional Oligodendrocytes Using Synthetic Modified Olig2 mRNA
Authors: Xu Jian , Wang Rui , Zhao Jingxin , He Fumei , Zhang Yidi , Xie Yuan , Shen Lan , Nie Yichu , Deng Wenbin 
Published: January 2022

Abstract
Background: Transcription factors (TFs) have been introduced to drive highly efficient differentiation of human induced pluripotent stem cells (hiPSCs) into lineage-specific oligodendrocytes (OLs). However, effective strategies currently rely mainly on genome-integrating viruses. To facilitate the translation of hiPSC-derived OLs into clinical practice, a synthetic modified messenger RNA (smRNA) reprogramming method that generates transgene-free OLs has been developed.Methods: An smRNA encoding Olig2, a key TF in OL development, with a defined phosphorylation site modification serine 147 replaced with alanine, Olig2S147A, was designed to reprogram hiPSCs into OLs. Proteomics were used to identify Olig2S147A-binding proteins that positively mediated of Olig2S147A-driven OL differentiation.Results: We demonstrated that repeated administration of the smRNA encoding Olig2 S147A led to higher and more stable protein expression. Using the single-mutant Olig2 smRNA with morphogens, we established a 6-day smRNA transfection protocol, and glial induction led to rapid NG2+ OL progenitor cell (OPC) generation (>70% purity) from hiPSC-derived neural progenitor cells (NPCs). The smRNA-induced NG2+ OPCs matured into functional OLs and myelinated nanofibers in vitro. Moreover, when transplanted into mice with cuprizone-induced demyelination, smRNA-induced OPCs promoted remyelination ex vivo. A proteomic analysis of Olig2-binding proteins indicated that the heat shock protein 70 (HSP70) complex bound Olig2. The HSP70 complex bound more strongly to Olig2 with the modified phosphorylation site than to wild type Olig2. VER-155008, an HSP70 complex antagonist, and ML346, an HSP70 complex agonist, inhibited and promoted Olig2 transcriptional activity and efficient OL generation, respectively.Conclusions: We present a very safe and efficient smRNA-driven strategy for hiPSC differentiation into OLs, which might be utilized for disease modeling, drug discovery, and/or therapeutic OPC/OL transplantation in neurodegenerative disease.
The Shortest Innovative Process of Black And Golden Garlic For Enhancing The S-Allylcysteine Content And Antioxidant Activity
Authors: Pakakaew Peeraporn , Phimolsiripol Yuthana , Taesuwan Siraphat , Kumphune Sarawut , Klangpetch Wannaporn , Utama-Ang Niramon 
Published: January 2022

Abstract
Black garlic is a type of heat-treated garlic for which the traditional process is extremely simple but it is very time-consuming, taking more than one month. The purpose of this research was to reduce the processing time of black garlic. A novel processing including soaking and freezing was applied for the pre-treatment of the garlic. The effects of two temperatures (60 and 80 °C) and two relative humidities (65 and 80% RH) were investigated to find the optimum conditions for black garlic production. The optimum conditions for black garlic production were pre-treatment using CaCl2 soaking followed by freezing before being further incubated at 80 °C and 80% RH for 1 week. This optimal condition produced black garlic with high antioxidant activity as 5,390 and 25,421 mg Trolox/100 g dry weight for DPPH and ABTS assays, respectively. However, the incubation process at 60 °C and 65% RH provided golden garlic with the highest S-allylcysteine (SAC) content by about 1,772 mg/100 g dry weight. This novel process shortened the processing time of black garlic by about four times and provided a new processed garlic product called “golden garlic”.
Gamma Knife Radiosurgery Versus Transcatheter Arterial Chemoembolization for Hepatocellular Carcinoma With Portal Vein Tumor Thrombus: a Propensity Score Matching Study
Authors: Su Ke , Gu Tao , Xu Ke , Wang Jing , Liao Hongfei , Li Xueting , Wen Lianbin , Song Yanqiong , Zhong Jiaqi , He Bingsheng , Liu Xin , He Jie , Liu Yanlin , Li Qi , Feng Xunjie , Chen Siyu , Yang Binbin , Huang Weihong , Jin Hongping , Luo Xiaotong , Hu Teng , Chen Jiali , Wu Zhenying , Lu Simin , Zhang Jianwen , Rao Mingyue , Xie Yunchuan , Wang Jing , Zhu Xiaoning , Chen Lan , Li Bo , Su Song , Yang Xiaoli , Wang Juan , Zeng Hao , Wang Pan , Yan Min , Chen Xiaojing , He Kun , Han Yunwei 
Published: January 2022

Abstract
BackgroundThe optimal management for hepatocellular carcinoma (HCC) patients with portal vein tumor thrombus (PVTT) is unclear. This study aimed to investigate the efficacy of Gamma knife radiosurgery (GKR) versus transcatheter arterial chemoembolization (TACE) in HCC patients with PVTT. MethodsThis retrospective study included 572 HCC patients with PVTT (GKR, 207; TACE, 365). Propensity score matching (PSM) analysis identified 188 matched pairs of patients. The primary endpoint was overall survival (OS).ResultsBefore PSM, the GKR group demonstrated significantly median OS (mOS) than TACE group (17.4 vs. 8.0 months, p < 0.001). In the subgroup analysis, GKR was associated with significantly longer OS for patients with PVTT II-IV (17.5 vs. 8.2 months, p < 0.001; 19.3 vs. 7.4 months, p < 0.001; 14.5 vs. 6.6 months, p = 0.008, respectively) and comparable OS for patients with PVTT I. After PSM, the GKR group had also a longer OS (mOS) than TACE group (15.8 vs. 9.8 months, p < 0.001). In the subgroup analysis, the GKR group demonstrated superior mOS for patients with PVTT II-IV (all P < 0.05) and comparable OS for patients with PVTT I. ConclusionsGKR was associated better OS than TACE in HCC patients with PVTT, especially for patients with PVTT II-IV.
MLC1: A New Calcium-regulated Protein Conferring Calcium Dependence to Volume-regulated Anion Channels (VRAC) in Astrocytes.
Authors: Brignone Maria Stefania , Lanciotti Angela , Michelucci Antonio , Mallozzi Cinzia , Camerini Serena , Catacuzzeno Luigi , Sforna Luigi , Caramia Martino , D'Adamo Maria Cristina , Ceccarini Marina , Molinari Paola , Macioce Pompeo , Macchia Gianfranco , Petrucci Tamara Corinna , Pessia Mauro , Visentin Sergio , Ambrosini Elena 
Published: January 2022

Abstract
MLC1 is a membrane protein highly expressed by brain perivascular astrocytes. Mutations in the MLC1 gene account for megalencephalic leukoencephalopathy with subcortical cysts (MLC), an incurable leukodystrophy characterized by macrocephaly, brain edema and cysts, myelin vacuolation and astrocyte swelling, causing cognitive and motor dysfunctions. It has been demonstrated that MLC1 mutations affect the swelling-activated Cl - currents (I Cl,swell ) mediated by volume-regulated anion channel (VRAC) and the consequent regulatory volume decrease (RVD) and lead to abnormal activation of intracellular signaling pathways linked to inflammation/osmotic stress. Despite this knowledge, the MLC1 physiological role and MLC molecular pathogenesis are still elusive. Following the observations that Ca 2+ regulates all the MLC1-modulated processes and that intracellular Ca 2+ homeostasis is altered in MLC1-defective cells, we applied a multidisciplinary approach including biochemistry, molecular biology, video imaging, electrophysiology and proteomic techniques on cultured astrocytes to uncover new Ca 2+ -dependent signaling pathways controlling MLC1 function. Here, we revealed that MLC1 binds the Ca 2+ effector proteins calmodulin (CaM) and Ca 2+ /CaM-dependent protein kinase II (CaMKII) and, as result, changes its assembly, localization and functional properties in response to Ca 2+ changes. Noteworthy, CaM binding to the COOH terminal promotes MLC1 trafficking to the plasma membrane, while CaMKII phosphorylation of the NH 2 -terminal potentiates MLC1 activation of I Cl,swell . Overall, these results revealed that MLC1 is a Ca 2+ -regulated protein linking VRAC function and, possibly, volume regulation to Ca 2+ signaling in astrocytes. These findings open new avenues of investigations aimed at clarifying the abnormal molecular pathways underlying MLC and other diseases characterized by astrocyte swelling and brain edema.
The Effectiveness of Governmental Nonpharmaceutical Interventions Against COVID-19 On The Control of Seasonal Influenza Transmission: An Ecological Study
Authors: Qiu Zekai , Cao Zicheng , Zou Min , Tang Kang , Zhang Chi , Tang Jing , Zeng Jinfeng , Wang Yaqi , Sun Qianru , Wang Daoze , Du Xiangjun 
Published: January 2022

Abstract
Background: A range of strict nonpharmaceutical interventions (NPIs) had been implemented in many countries to combat the COVID-19 pandemic. These NPIs might also be effective in controlling the seasonal influenza virus, which share the same transmission path with SARS-CoV-2. The aim of this study is to evaluate the effect of different NPIs for control of seasonal influenza.Methods: Data on 14 NPIs implemented in 33 countries and corresponding data on influenza virologic surveillance were collected. The influenza suppression index was calculated as the difference between the influenza-positive rate during its decline period from 2019 to 2020 and that during influenza epidemic seasons in the previous 9 years. A machine learning model was developed by using extreme gradient boosting tree (XGBoost) regressor to fit NPI data and influenza suppression index. SHapley Additive exPlanations (SHAP) was used to characterize NPIs in suppressing influenza.Results: Gathering limitation contributed the most (37.60%) among all NPIs in suppressing influenza transmission in the 2019-2020 influenza season. The top three effective NPIs were gathering limitation, international travel restriction, and school closure. Regarding the three NPIs, their intensity threshold to generate effect were restrictions on the size of gatherings less than 1000 people, travel bans on all regions or total border closure, and closing only some categories of schools, respectively. There was a strong positive interaction effect between mask wearing requirement and gathering limitation, whereas merely implementing mask wearing requirement but ignoring other NPIs would dilute mask wearing requirement’s effectiveness in suppressing influenza.Conclusions: Gathering limitation, travel bans on all regions or total border closure, and closing some levels of schools are the most effective NPIs to suppress influenza transmission. Mask wearing requirement is advised to be combined with gathering limitation and other NPIs. Our findings could facilitate the precise control of future influenza epidemics and potential pandemics.
Metabolic Stress Index Including Mitochondrial Biomarker for Noninvasive Diagnosis of Hepatic Steatosis
Authors: Chang Jae Seung , Ahn Jhii-Hyun , Kang Seong Hee , Koh Sang-Baek , Kim Jang-Young , Baik Soon Koo , Huh Ji Hye , Lee Samuel S. , Kim Moon Young , Park Kyu-Sang 
Published: January 2022

Abstract
Background Mitochondrial dysfunction with oxidative stress contributes to nonalcoholic fatty liver disease (NAFLD) progression. We investigated the steatosis predictive efficacy of a novel non-invasive diagnostic panel using metabolic stress biomarkers. Methods Altogether, 343 subjects who underwent magnetic resonance imaging-based liver examinations from a population-based general cohort, and 41 patients enrolled in a biopsy-evaluated NAFLD cohort, participated in the development and validation groups, respectively. Serologic stress biomarkers were quantitated by enzyme-linked immunosorbent assay. Results Multivariate regression showed that waist-to-hip ratio, fibroblast growth factor (FGF) 21, FGF19, adiponectin-to-leptin ratio, insulin, albumin, triglyceride, total-cholesterol, and alanine-aminotransferase were independent predictors of steatosis (rank-ordered by Wald). The area under receiver-operator characteristics curve [AUROC (95%CI)] of the metabolic stress index for steatosis (MSI-S) was 0.886 (0.85‒0.92) and 0.825 (0.69‒0.96) in development and validation groups, respectively. MSI-S had higher diagnostic accuracy (78.1%‒81.1%) than other steatosis indices. MSI-S notably differentiated steatosis severities, while other indices showed less discrimination. Conclusion MSI-S, as a novel non-invasive index, based on mitochondrial stress biomarker FGF21 effectively predicted steatosis. Furthermore, MSI-S may increase the population that could be excluded from further evaluation, reducing unnecessary invasive investigations more effectively than other indices.
First DNA Barcoding Based Record of Lysiosquillina Maculata (Fabricius, 1793) (Crustacea: Stomatopoda) From Chennai Coast, Tamil Nadu, India
Authors: C Babu , K Silambarasan , A Anrose , A Tiburtius 
Published: January 2022

Abstract
Taxonomic identification of mantis shrimp Lysiosquillina maculata through DNA barcoding analysis collected from Kasimedu fisheries harbour, Chennai coast, Tamil Nadu, India. The mitochondrial cytochrome oxidase sub unit I gene (mtcoI) with 650 bp region was sequenced for phylogenetic analysis. The present record, mitochondrial gene sequences were used to identify the mantis shrimp Lysiosquillina maculata. This is the first confirmed record of Indian waters and the mt COI sequence was deposited in GenBank. The neighbor joining method was used for phylogenetic analysis. The pair wise genetic distance calculated with 08 closely related species varied form 0.03-0.404%. Phylogenetic tree based on 13 protein coding genes shows that Lysiosquillina maculata has a closer phylogenetic relationship to Harpiosquilla harpax.
Parent Moral Distress From The Perspectives of Mothers With An Infant Previously Hospitalized in The NICU
Authors: Voultsos Polychronis , Deligianni Maria , Tsamadou Eftychia 
Published: January 2022

Abstract
BackgroundGiven that having an infant in the NICU is a morally stressful event for parents and preterm birth rates are increasing worldwide, parent moral distress is a matter of public health. While moral distress in the clinical context is already a widely explored phenomenon, the parent moral distress still remains a largely under-explored complex phenomenon. Methods This is a prospective qualitative study using semi-structured interviews of seventeen parents (mothers) who previously had an infant in the NICU. The study conducted between February 2021 and August 2021. A thematic analysis of the data was performed. Results While parents with infants in the NICU initially needed for having their decisions respected by physicians, they ultimately shew a tolerant attitude towards benevolent medical paternalism. However, many physician-related factors (i.e. uncertainty, discrepancy of opinion, operational behavior, lack of communicative or empathetic behavior, previous malpractice) facilitate parent moral distress. The important role of the family pediatrician in creating parent moral distress is highlighted. The same holds for family/social environment – related factors. Furthermore, the role of parent’s internal factors (especially spiritual/existential core values or beliefs) as well as the role of NICU environment-related factors (such as the image of a long-suffering infant, preventing mothers from providing their own milk for their infants and having a skin-to-skin contact with them) in creating parent moral distress are highlighted. Moral residue no more than mild was found. Moreover, parent moral schisma is emerged as a conceptually distinct from moral distress phenomenon. Last, the findings of this study confirm the relational account of parental moral distress offered by Mooney-Doyle and Ulrich. ConclusionsWhile many findings of the present study are in line with previous studies, our data analysis revealed findings which are little recognized in the available literature. Parents showed tolerant attitudes towards benevolent medical paternalism. No more than mild moral residue has been found. The most relevant categories of variables associated with parent moral distress were a) physician-related (various factors), b) parent-related, c) parent’s context (family or broader social)-related, and d) NICU environment-related. Furthermore, parent moral distress and parent moral schisma are conceptualized as conceptually distinct albeit strictly related or overlapping phenomena. The findings of this study support the relational account of parent moral distress.