bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of polycarbodiimide chain extender on thermal stability and mechanical properties of biobased poly(lactic acid)/natural rubber blown films
Authors: Akaradechakul Kornchanok , Chanthot Peerapong , Kerddonfag Noppadon , Pattamaprom Cattaleeya 
Published: March 2022

This is the first study investigating how polycarbodiimide (PCDI) delays thermal degradation of poly(lactic acid)/natural rubber blends (PLA/NR) during the preparation of PLA/NR masterbatches (MB) and PLA/MB compounds for the film blowing process. The PCDI concentration used in this work was from 0 to 1 wt%. The torque plastograms, FTIR, and 1H-NMR spectra indicated the successful chain extending reaction between the PCDI carbodiimide group and the PLA carboxyl group, and there was no reaction with NR domains. The reaction increased the PLA/MB compounds’ viscosity and improved processability and mechanical properties of PLA/MB blown films. The optimal PCDI content for the highest melt strength, film blowing stability, and mechanical properties was at 0.25 wt% PCDI. Undesirable gel spots were observed in the PLA/MB blown film at PCDI concentrations higher than 0.5 wt%.
Efficacy and safety of CD19-specific CAR T cell–based therapy in B-cell acute lymphoblastic leukemia patients with CNSL
Authors: Qi Yuekun , Zhao Mingfeng , Hu Yongxian , Wang Ying , Li Ping , Cao Jiang , Shi Ming , Tan Jiaqi , Zhang Meng , Xiao Xia , Xia Jieyun , Ma Sha , Qiao Jianlin , Yan Zhiling , Li Hujun , Pan Bin , Sang Wei , Li Depeng , Li Zhenyu , Zhou Jianfeng , Huang He , Liang Aibin , Zheng Junnian , Xu Kailin 
Published: March 2022

Abstract
Few studies have described chimeric antigen receptor (CAR) T-cell therapy for patients with B-cell acute lymphoblastic leukemia (B-ALL) with central nervous system leukemia (CNSL) because of concerns regarding poor response and treatment-related neurotoxicity. Our study included 48 patients with relapsed/refractory B-ALL with CNSL to evaluate the efficacy and safety of CD19-specific CAR T cell–based therapy. The infusion resulted in an overall response rate of 87.5% (95% confidence interval [CI], 75.3-94.1) in bone marrow (BM) disease and remission rate of 85.4% (95% CI, 72.8-92.8) in CNSL. With a median follow-up of 11.5 months (range, 1.3-33.3), the median event-free survival was 8.7 months (95% CI, 3.7-18.8), and the median overall survival was 16.0 months (95% CI, 13.5-20.1). The cumulative incidences of relapse in BM and CNS diseases were 31.1% and 11.3%, respectively, at 12 months (P = .040). The treatment was generally well tolerated, with 9 patients (18.8%) experiencing grade ≥3 cytokine release syndrome. Grade 3 to 4 neurotoxic events, which developed in 11 patients (22.9%), were associated with a higher preinfusion disease burden in CNS and were effectively controlled under intensive management. Our results suggest that CD19-specific CAR T cell–based therapy can induce similar high response rates in both BM and CNS diseases. The duration of remission in CNSL was longer than that in BM disease. CD19 CAR T-cell therapy may provide a potential treatment option for previously excluded patients with CNSL, with manageable neurotoxicity. The clinical trials were registered at www.clinicaltrials.gov as #NCT02782351 and www.chictr.org.cn as #ChiCTR-OPN-16008526.
Functional mapping of PHF6 complexes in chromatin remodeling, replication dynamics, and DNA repair
Authors: Alvarez Silvia , da Silva Almeida Ana C. , Albero Robert , Biswas Mayukh , Barreto-Galvez Angelica , Gunning Thomas S. , Shaikh Anam , Aparicio Tomas , Wendorff Agnieszka , Piovan Erich , Van Vlierberghe Pieter , Gygi Steven , Gautier Jean , Madireddy Advaitha , A. Ferrando Adolfo 
Published: March 2022

Abstract
The Plant Homeodomain 6 gene (PHF6) encodes a nucleolar and chromatin-associated leukemia tumor suppressor with proposed roles in transcription regulation. However, specific molecular mechanisms controlled by PHF6 remain rudimentarily understood. Here we show that PHF6 engages multiple nucleosome remodeling protein complexes, including nucleosome remodeling and deacetylase, SWI/SNF and ISWI factors, the replication machinery and DNA repair proteins. Moreover, after DNA damage, PHF6 localizes to sites of DNA injury, and its loss impairs the resolution of DNA breaks, with consequent accumulation of single- and double-strand DNA lesions. Native chromatin immunoprecipitation sequencing analyses show that PHF6 specifically associates with difficult-to-replicate heterochromatin at satellite DNA regions enriched in histone H3 lysine 9 trimethyl marks, and single-molecule locus-specific analyses identify PHF6 as an important regulator of genomic stability at fragile sites. These results extend our understanding of the molecular mechanisms controlling hematopoietic stem cell homeostasis and leukemia transformation by placing PHF6 at the crossroads of chromatin remodeling, replicative fork dynamics, and DNA repair.
Randomized controlled trial of all-inside and standard single-bundle anterior cruciate ligament reconstruction with functional, MRI-based graft maturity and patient-reported outcome measures
Authors: Lin Rubing , Zhong Qiuwen , Wu Xiao , Cui Lei , Huang Rong , Deng Qianhua , Zuo Jianwei , Jiang Changqing , Li Wei 
Published: March 2022

Abstract
Background
All-inside anterior cruciate ligament reconstruction (ACLR) is a novel technique that has gained attention due to its minimally invasive and graft-saving properties. However, studies comparing MRI-based graft maturity between all-inside and standard ACLR are lacking.

Purpose
This study focused on the functional, knee laxity, and MRI-based graft maturity characteristics of all-inside and standard single-bundle ACLR.

Study Design
Randomized controlled trial (RCT).

Methods
Fifty-four patients were randomly assigned to an all-inside reconstruction group (n = 27) or standard reconstruction group (n = 27). Using the same rehabilitation strategy. The Tegner, International Knee Documentation Committee, and Lysholm scores were recorded at postoperative months 3, 6, and 12 to assess functional recovery. MRI was conducted to measure the signal/noise quotient (SNQ) of the intra-articular graft to assess the maturity. A higher SNQ indicates lower graft maturity. Knee laxity was assessed using GNRB arthrometer at the postoperative month 12.

Results
The graft SNQ of the all-inside group was significantly higher than that of the standard group at postoperative month 6 (p < 0.05). There was no statistical difference in graft SNQ between the two groups at postoperative months 3 and 12 (p > 0.05). Both groups exhibited the highest SNQ in the middle region of the graft, followed by the proximal region, and the distal region. Functional scores improved significantly for both groups and had no statistical difference (p > 0.05). The knee laxity was higher in the all-inside group (p < 0.05) at postoperative month 12. There was no correlation between the functional scores and graft maturity in both groups (p > 0.05).

Conclusions
All-inside and standard single-bundle ACLR show good functional outcomes; however, knee laxity was relatively higher in the all-inside ACLR group than in the standard ACLR group. Moreover, both techniques exhibited poor maturity in the middle graft region and the best in the distal region. Graft maturity with all-inside ACLR is inferior to that with standard ACLR in the early postoperative stages. There is no correlation between knee function and graft maturity.

Trial registration
Clinical trial registration numbers: ChiCTR1800018543.
Date of registration: 09/23/2018.
A focus group interview with health professionals: establishing efficient transition care plan for older adult patients in Korea
Authors: Park Chan Mi , Han Seung Jun , Lee Jae Hyun , Lim Jin , do Moon Sung , Moon Hongran , Lee Seo-Young , Kim Hyeanji , Jang Il-Young , Jung Hee-Won 
Published: March 2022

Abstract
Background
Although transition care planning can affect the functional status and quality of life after acute hospitalization in older adults, little is known on problems associated with discharge planning in acute care hospitals in Korea. We aimed to investigate barriers and possible solutions on transfer planning of complex older patients in this study.

Methods
We used focus group interviews with the application of framework analysis. Twelve physicians providing inpatient care from 6 different institutions in Korea participated in the interview. Facilitating questions were extracted from 2 roundtable meetings prior to the primary interview. From transcribed verbatim, themes were constructed from corresponding remarks by participants.

Results
We revealed two main domains of the barrier, which included multiple subdomains for each of them. The first domain was a patient factor barrier, a composite of misperception of medical providers’ intentions, incomprehension of the healthcare system, and communication failure between the caregivers or decision-makers. The second domain, institutional factors included different fee structures across the different levels of care, high barrier to accessing health service in tertiary hospitals or to be referred to, the hardship of communication between institutions, and insufficient subacute rehabilitation service across the country.

Conclusions
Through the interview, physicians in the field recognized barriers to a smooth transition care process from tertiary level hospitals to community care, especially for older adults. Participants emphasized both the patients and hospital sides of adjustment on behaviors, communication, and greater attention for the individuals during the transition period.
Rasmussen’s encephalitis is characterized by relatively lower production of IFN-β and activated cytotoxic T cell upon herpes viruses infection
Authors: Wang Yi-Song , Liu Dong , Wang Xin , Luo Qiao-Li , Ding Ling , Fan Dong-Ying , Cai Qi-Liang , Tang Chong-Yang , Yang Wei , Guan Yu-Guang , Li Tian-Fu , Wang Pei-Gang , Luan Guo-Ming , An Jing 
Published: March 2022

Abstract
Background
The etiology of Rasmussen's encephalitis (RE), a rare chronic neurological disorder characterized by CD8+ T cell infiltration and unihemispheric brain atrophy, is still unknown. Various human herpes viruses (HHVs) have been detected in RE brain, but their contribution to RE pathogenesis is unclear.

Methods
HHVs infection and relevant immune response were compared among brain tissues from RE, temporal lobe epilepsy (TLE) and traumatic brain injury (TBI) patients. Viral antigen or genome, CD8+ T cells, microglia and innate immunity molecules were analyzed by immunohistochemical staining, DNA dot blot assay or immunofluorescence double staining. Cytokines were measured by multiplex flow cytometry. Cell apoptosis was visualized by TUNEL staining. Viral infection, immune response and the severity of unihemispheric atrophy were subjected to correlation analysis.

Results
Antigens of various HHVs were prevalent in RE and TLE brains, and the cumulative viral score of HHVs positively correlated with the unihemispheric atrophy in RE patients. CD8+ T cells infiltration were observed in both RE and TLE brains and showed co-localization with HHV antigens, but their activation, as revealed by Granzyme B (GZMB) release and apoptosis, was found only in RE. In comparison to TLE, RE brain tissues contained higher level of inflammatory cytokines, but the interferon-β level, which was negatively correlated with cumulative viral score, was relatively lower. In line with this, the DNA sensor STING and IFI16, rather than other innate immunity signaling molecules, were insufficiently activated in RE.

Conclusions
Compared with TBI, both RE and TLE had prevalently HHV infection and immune response in brain tissues. However, in comparison to TLE, RE showed insufficient activation of antiviral innate immunity but overactivation of cytotoxic T cells. Our results show the relatively lower level of antiviral innate immunity and overactivation of cytotoxic T cells in RE cases upon HHV infection, the overactivated T cells might be a compensate to the innate immunity but the causative evidence is lack in our study and need more investigation in the future.
Moderate phenotype of a congenital myasthenic syndrome type 19 caused by mutation of the COL13A1 gene: a case report
Authors: Kediha Mohamed Islam , Tazir Meriem , Sternberg Damien , Eymard Bruno , Alipacha Lamia 
Published: March 2022

Abstract
Background
Congenital myasthenic syndromes caused by mutations in the COL13A1 gene are very rare and have a phenotype described as severe. We present the first case of congenital myasthenic syndrome described in Algeria and the Maghreb with a new mutation of this gene.

Case presentation
We present an 8-year-old Algerian female patient, who presented with a moderate phenotype with bilateral ptosis that fluctuates during the day and has occurred since birth. During the investigation, and despite the very probable congenital origin, we ruled out other diagnoses that could induce pathology of the neuromuscular junction. The genetic study confirmed our diagnosis suspicion by highlighting a new mutation in the COL13A1 gene.

Conclusion
We report a case with a mutation of the Col13A1 gene, reported in the Maghreb (North Africa), and whose phenotype is moderate compared with the majority of cases found in the literature.
Poly-l-lysine-caused cell adhesion induces pyroptosis in THP-1 monocytes
Authors: Yu Chaoping , Zhao Wei , Duan Chujun , Xie Jiangang , Yin Wen 
Published: March 2022

Abstract
Pyroptosis is a kind of cell necrosis mediated by inflammasomes. The caspase 1-induced cleavage of gasdermin D (GSDMD) is a canonical pathway to cause membrane pores and eventually cell pyroptosis. Poly-l-lysine (PLL) is widely used to enhance cell adhesion during experiments. Human THP-1 cells are a typical cell line used to study pyroptosis due to their monocytic and macrophage-like characteristics. However, it was found that THP-1 cells seeded on the PLL-coated slides died. To figure out the reason, we observed the morphology of THP-1 cells on PLL-coated slides, which showed obvious pore forming on the cell membranes and cell swelling. The indicated pyroptosis-related protein expression was evaluated and it showed that the conventional caspase-1 pathway of pyroptosis was activated through the NLRP3 inflammasome in THP-1 monocytes on the PLL-coated slides. Hence, PLL-guided cell adhesion induces cell pyroptosis in THP-1 monocytes, which calls for THP-1 dominant studies of pyroptosis to avoid the use of PLL-coated slides or PLL-related drugs.
circ-CFH promotes the development of HCC by regulating cell proliferation, apoptosis, migration, invasion, and glycolysis through the miR-377-3p/RNF38 axis
Authors: Chen Zengyin , Du Juan , Yang Chen , Si Guangju , Chen Yuxin 
Published: March 2022

Abstract
Circular RNAs (circRNAs) have previously been confirmed to function as vital regulators in multiple human cancers, including hepatocellular carcinoma (HCC). This study aimed to clarify the role and underlying molecular mechanisms of circ-CFH in HCC. circ-CFH was overexpressed in HCC tissues and cells, and the downregulation of circ-CFH inhibited the development of HCC by repressing cell proliferation, migration, invasion, and glycolysis while enhancing apoptosis in vitro, as well as inhibited tumor growth in vivo. miR-377-3p was negatively regulated by circ-CFH, and silencing of miR-377-3p abolished circ-CFH knockdown-mediated effects on HCC cells. Moreover, overexpression of miR-377-3p could impede the HCC process by targeting RNF38. Mechanistically, the circ-CFH/miR-377-3p/RNF38 axis regulated the progression of HCC cells, which might provide new diagnostic markers for HCC.
Influence of biosynthesized silver nanoparticles using red alga Corallina elongata on broiler chicks’ performance
Authors: El-Abd Niamat M. , Hamouda Ragaa A. , Al-Shaikh Turki M. , Abdel-Hamid Marwa Salah 
Published: March 2022

Abstract
Poultry meat is a great source of protein and provides lots of nutrients such as iodine, iron, zinc, vitamins, and essential fatty acids that humans require. The positive applications of metal nanoparticles (NPs) in the diets of various poultry species were studied, in relation to their metabolic, antibacterial effects on digestion and regulation of bowel function. This study was carried out to test the effects of fabrication green silver nanoparticles (AgNPs) of Corallina elongata extract and/or coating NPs with acetic acid on performance, immune response parameters and micro-flora population in Ross broiler. Chicks’ drinking water was mixed with bio-AgNPs (1 mM) and coating NPs with acetic acid for 35 days. Fourier-transform infrared spectroscopy, electron dispersive spectroscopy (EDS) analysis, scanning electron microscopy, and high resolution transmission electron microscope were used to determine the partial physiochemical characterizations of bio-AgNPs and coating ones. EDS analysis was used to determine the presence of AgNP in meat. Results confirmed that coating NPs with acetic acid reduced percentage of the micro-flora population, which were detected by VITEK® 2 system (BioMérieux, France) and identified as Pseudomonas orizihabitain 4211210040000210 and Sphinogomonas paucimobilis 5201210040000210. EDS analysis of meat chicks confirmed disappearance of Ag metals. Coating biogenic AgNPs with acetic acid on modulated intestinal microbial populations of the Ross broiler may be safe, and could be used as alternative antibiotics or antibacterial agents besides their physiological performance in small intestines of broiler chicken.
Effect of post-heat treatment on fire retardant treated wood properties
Authors: Özkan Osman Emre , Temiz Ali , Tor Önder , Vurdu Hasan 
Published: March 2022

Abstract
In this study, Anatolian black pine (Pinus nigra subsp. pallasiana) was treated with fire retardants of 10, 20, and 30% aqueous solutions composed of di-ammonium phosphate (DAP), borax, boric acid, and glucose to determine the effectiveness of these chemicals in preventing the spread of fire. The treated wood was subjected to post-heat treatment at 120, 150, and 180 °C for 1 h to examine the effects of the post-heat treatment on pH changes, anti-swelling efficiency, water absorption, mechanical strength, accelerated weathering, leaching resistance, thermogravimetric analysis (TGA), UL-94 vertical burning test and crystallinity index. The post-heat treatment of fire-retardant treated wood reduced water intake and increased dimensional stability, while also improving mechanical and thermal properties in general. DAP/glucose complexes promote phosphorus fixation in wood and reduce leaching, resulting in long-term fire protection of wood in service. Because of these improvements in the post-heat treated wood by impregnated fire retardant, it can potentially be used as a reliable engineering material in a wide range of structural applications.
No association of the common Asian mitochondrial DNA haplogroups with lung cancer in East Indian population
Authors: Saha Tania , Bhowmick Bismoy , Sengupta Debmalya , Banerjee Souradeep , Mitra Ritabrata , Sarkar Abhijit , Chaudhuri Tamohan , Bhattacharjee Gautam , Nath Somsubhra , Roychoudhury Susanta , Sengupta Mainak 
Published: March 2022

Abstract

Objectives
Mitochondrial dysfunction has long been associated with the pathogenesis of lung cancer (LC). Mitochondrial DNA (mtDNA) haplogroups have been reported to modify the risk of LC in a few different populations; however, no study has been done among the Indians. Here, we explore the relationship between mtDNA haplogroups and LC in a representative eastern Indian sample set.

Methods
Different combinations of six mtDNA SNPs, which define the major Asian mtDNA haplogroups M and N, and their sub-haplogroups D, G, M7, R, and F were genotyped via polymerase chain reaction (PCR) – restriction fragment length polymorphism (RFLP) – sequencing approach in 94 smoker LC patients and 100 healthy smoker controls from an eastern Indian cohort.

Results
The distribution of 7 mtDNA haplogroups did not show any significant differences between patients and controls (p<0.05). We did not find sub-haplogroup M7 in our study population.

Conclusions
Our study is the first to indicate that the major Asian mtDNA haplogroups have no significant (p<0.05) association with LC in East Indian population.
Türkiye’de enerji ithalatı ile cari açık ilişkisinin 2010-2019 dönemi için ampirik analizi
Authors: İnançlı Selim , Akı Aylin 
Published: March 2022

Son yüzyılda dünyada üretim faaliyetlerinin artması ile birlikte enerjiye olan talepte hızlı artış görülmüştür. Enerji arz kaynakları açısından yetersiz olan ülkeler enerji talebini karşılamak için enerji ithalatlarını arttırmıştır. Dünyada enerji talebinde ve döviz kurundaki artışlar enerji fiyatlarının yükselmesine sebep olmuş ve gelişme yolundaki ülkelerin enerji ithalatına daha fazla pay ayırmalarına yol açmıştır. Enerji arz kaynakları açısından yetersiz olan Türkiye, enerji ithalatına büyük oranda harcama yapmaktadır. Gelişme yolunda ülkelerde ve Türkiye’ de artan enerji ithalatı cari açığın en önemli nedenlerinden biri durumundadır. Bu makalede Türkiye’nin enerji ithalatının cari açık üzerine etkileri “1990-2019” dönemi yıllık verilerle ampirik olarak analiz edilmektedir. Enerji ithalatı ve cari açık arasındaki ilişkiler ADF, PP ve LS çift kırılmalı birim kök testleri, Hacker ve Hatemi-J simetrik ve zamanla değişen nedensellik testleriyle tahmin edilmektedir. Sonuçta 1999 ile 2008 ve 2010 ile 2019 yılları arasında enerji ithalatından cari açığa, 2000 ile 2009 yılları arasında ise cari açıktan enerji ithalatına doğru nedensellik ilişkisi olduğu tespit edilmiştir.
Bilinçli tüketici kavramına ilişkin algıların değerlendirilmesi: Bir metafor analizi çalışması
Authors: Cici Karaboğa Emine Nihan 
Published: March 2022

Bu çalışmanın amacı bilinçli tüketici kavramının tüketici zihnindeki algısının metaforlar üzerinden değerlendirilmesinin yapılmasıdır. Sürdürülebilir bir yaşam döngüsünün kurulması için anahtar kavramlardan biri olan bilinçli tüketicinin dolayısıyla bilinçli tüketim faaliyetinin tüketici konumunda olan bireyler tarafından nasıl anlamlandırdığını tespit edilmek amaçlanmıştır. Bu nedenle nitel araştırma desenlerinden olgu bilim yaklaşımının tercih edildiği bu çalışmada evrenin Türkiye olarak seçildiği 109 tüketiciye ulaşılmıştır. Tüketicilerden “Bilinçli tüketici……………………gibidir/benzer. Çünkü…………” cümlesini metaforlar ile tamamlanmaları ve nedeninin açıklanması istenmiş, elde edilen veriler içerik ve betimsel analiz yöntemleri kullanılarak analiz edilmiştir. Analiz edilen verilerdeki 75 metafor 5 farklı kategoriye ayrılırken bu kategoriler literatür bağlamında değerlendirilmiştir. Çalışmada en çok tercih edilen/tanımlanan metaforlar; “tasarruflu olmak, çevreye duyarlı olmak, işletme müdür ve kumbara” olarak tespit edilmiştir.