bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Implementation of a Pilot Transition Preparation Intervention for Young Adults With Type 1 Diabetes in an Integrated Healthcare Setting
Authors: Caccavale Laura Jean , LaRose Jessica Gokee , Mazzeo Suzanne E , Bean Melanie K 
Published: November 2022

AbstractObjectiveTo evaluate the feasibility, acceptability, and preliminary efficacy of a multisystem transition preparation intervention, SHIFT, for young adults (YAs) with type 1 diabetes (T1D).MethodsA single-arm, clinic-based pilot was conducted with 25 YAs with T1D (M age = 18.9 ± 1.0 years; 80% female), their parents (n = 25), and their providers (n = 10). Young adults and parents participated in a 6-month intervention designed to enhance transition readiness and independent diabetes management. Providers viewed a video module highlighting their role preparing YAs for transition and received individualized reports of YA’s goals and transition readiness. Intervention feasibility (i.e., recruitment, retention, and engagement) and acceptability (e.g., program satisfaction) were assessed. Assessments of transition readiness, diabetes engagement, hemoglobin A1c (HbA1c), and related psychosocial variables were conducted at baseline, post-intervention (6 m), and follow-up (9 m). Paired t-tests examined 0–6 m and 0–9 m changes in study constructs.ResultsSHIFT was feasible, evidenced by recruitment (100% of sample recruited in 4 m), retention (100% at 6 m), and YA session attendance (100%). Program satisfaction was high for YAs, parents, and providers (9.12 ± 1.40, 8.79 ± 1.56, 8.20 ± 1.30, respectively, [out of 10]). Significant improvements (with effect sizes ranging from small to medium) were observed in parent and YA-reported transition readiness at 6 and 9 m (ps&lt;.05) and diabetes engagement at 9 m (ps&lt;.05). Although based on limited data due to COVID-19-related disruptions, a potential reduction in HbA1c was also observed.ConclusionFindings support the feasibility, acceptability, and preliminary efficacy of SHIFT (although limited by the single arm design and homogeneous sample), and suggest a larger randomized controlled trial is warranted.
N(Omega)-nitro-l-arginine methyl ester potentiates lidocaine analgesic and anaesthetic effect in rats
Authors: Chou An-Kuo , Chen Yu-Wen 
Published: November 2022

Abstract

Objectives
The purpose of the experiment was to study the effect of L-NAME (N(Omega)-nitro-L-arginine methyl ester) and its cotreatment with lidocaine on the spinal block and infiltrative cutaneous analgesia.

Methods
The quality of cutaneous analgesia was examined by the block of the cutaneous trunci muscle reflexes following needle stimuli in the rat. Spinal anaesthetic potency was assessed by measuring three neurobehavioral examinations of nociceptive, proprioceptive and motor function following intrathecal injection in the rat.

Key findings
L-NAME (0.6, 6 and 60 nmol) when cotreatment with lidocaine (ED50) produced dose-related cutaneous analgesia. Coadministration of L-NAME (0.6 μmol) with lidocaine intensified (P &lt; 0.01) and prolonged (P &lt; 0.001) cutaneous analgesia, whereas subcutaneous L-NAME (0.6 μmol) and saline did not provoke cutaneous analgesic effects. Adding L-NAME (2.5 μmol) to lidocaine intrathecally prolonged spinal sensory and motor block (P &lt; 0.01), while intrathecal L-NAME (2.5 μmol) or 5% dextrose (vehicle) produced no spinal block.

Conclusions
L-NAME at 60 nmol (the minimum effective dose) increases and prolongs the effect of cutaneous analgesia of lidocaine. L-NANE at an ineffective dose potentiates lidocaine analgesic and anaesthetic effects.
Cold gas mass measurements for the era of large optical spectroscopic surveys
Authors: Scholte Dirk , Saintonge Amélie 
Published: November 2022

ABSTRACT
Gas plays an important role in many processes in galaxy formation and evolution, but quantifying the importance of gas has been hindered by the challenge to measure gas masses for large samples of galaxies. Data sets of direct atomic and molecular gas measurements are sufficient to establish simple scaling relations, but often not large enough to quantify three-parameter relations, or second-order dependences. As an alternative approach, we derive here indirect cold gas measurements from optical emission lines using photoionization models for galaxies in the Sloan Digital Sky Survey (SDSS) main galaxy sample and the PHANGS-MUSE survey. We calibrate the gas surface density measurements using xCOLD GASS and PHANGS-ALMA molecular gas measurements to ensure that our measurements are reliable. We demonstrate the importance of taking into account the scale dependence of the relation between optical depth (τV) and gas surface density (Σgas) and provide a general prescription to estimate Σgas from τV, metallicity, and the dust-to-metal ratio, at any arbitrary physical resolution. To demonstrate that the indirect cold gas masses are accurate enough to quantify the role of gas in galaxy evolution, we study the mass–metallicity relation of SDSS galaxies and show that as a third parameter gas mass is better than star formation rate at reducing the scatter of the relation, as predicted by models and simulations.
In situdetection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
Authors: Jovanović Marić Jovana , Kolarević Stoimir , Đorđević Jelena , Sunjog Karolina , Nikolić Ivan , Marić Ana , Ilić Marija , Simonović Predrag , Alygizakis Nikiforos , Ng Kelsey , Oswald Peter , Slobodnik Jaroslav , Žegura Bojana , Vuković-Gačić Branka , Paunović Momir , Kračun-Kolarević Margareta 
Published: November 2022

AbstractEnvironmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
Decoding vesicle-based precision oncology in gliomas
Authors: Batool Syeda Maheen , Hsia Tiffaney , Khanna Sirena K , Gamblin Austin S , Rosenfeld Yulia , You Dong Gil , Carter Bob S , Balaj Leonora 
Published: November 2022

Abstract
Extracellular vesicles (EVs) represent a valuable tool in liquid biopsy with tremendous clinical potential in diagnosis, prognosis, and therapeutic monitoring of gliomas. Compared to tissue biopsy, EV-based liquid biopsy is a low-cost, minimally invasive method that can provide information on tumor dynamics before, during, and after treatment. Tumor-derived EVs circulating in biofluids carry a complex cargo of molecular biomarkers, including DNA, RNA, and proteins, which can be indicative of tumor growth and progression. Here, we briefly review current commercial and noncommercial methods for the isolation, quantification, and biochemical characterization of plasma EVs from patients with glioma, touching on whole EV analysis, mutation detection techniques, and genomic and proteomic profiling. We review notable advantages and disadvantages of plasma EV isolation and analytical methods, and we conclude with a discussion on clinical translational opportunities and key challenges associated with the future implementation of EV-based liquid biopsy for glioma treatment.
Prediagnostic biomarkers for early detection of glioma—using case–control studies from cohorts as study approach
Authors: Wu Wendy Yi-Ying , Dahlin Anna M , Wibom Carl , Björkblom Benny , Melin Beatrice 
Published: November 2022

Abstract

Background
Understanding the trajectory and development of disease is important and the knowledge can be used to find novel targets for therapy and new diagnostic tools for early diagnosis.

Methods
Large cohorts from different parts of the world are unique assets for research as they have systematically collected plasma and DNA over long-time periods in healthy individuals, sometimes even with repeated samples. Over time, the population in the cohort are diagnosed with many different diseases, including brain tumors.

Results
Recent studies have detected genetic variants that are associated with increased risk of glioblastoma and lower grade gliomas specifically. The impact for genetic markers to predict disease in a healthy population has been deemed low, and a relevant question is if the genetic variants for glioma are associated with risk of disease or partly consist of genes associated to survival. Both metabolite and protein spectra are currently being explored for early detection of cancer.

Conclusions
We here present a focused review of studies of genetic variants, metabolomics, and proteomics studied in prediagnostic glioma samples and discuss their potential in early diagnostics.
Blood platelets as an RNA biomarker platform for neuro-oncological diseases
Authors: Post Edward , Sol Nik , Best Myron G , Wurdinger Thomas 
Published: November 2022

Abstract
Blood-based liquid biopsies are an upcoming approach for earlier cancer detection, diagnostics, prognostics, therapy-response prediction, and therapy monitoring, including in patients with tumors of the central nervous system. Among these, liquid biopsies are plasma-derived markers such as cell-free DNA, RNA and proteins, extracellular vesicles, circulating glioma cells, immune cells, and blood platelets. Blood platelets are involved in the local and systemic response to the presence of cancer, thereby sequestering and splicing RNAs, which may be clinically useful as blood-based biomarkers. In this review, we discuss the available literature regarding the role of blood platelets in gliomas and provide suggestions for future research efforts.
TGF-β3 in differentiation and function of Tph-like cells and its relevance to disease activity in patients with systemic lupus erythematosus
Authors: Shan Yu , Nakayamada Shingo , Nawata Aya , Yamagata Kaoru , Sonomoto Koshiro , Tanaka Hiroaki , Satoh-Kanda Yurie , Nguyen Mai-Phuong , Todoroki Yasuyuki , Nagayasu Atsushi , Ueno Masanobu , Kanda Ryuichiro , Fujita Yuya , Zhang Tong , Hao He , Zhou Jieqing , Ma Xiaoxue , Anan Junpei , Nguyen Anh Phuong , Tanaka Yoshiya 
Published: November 2022

Abstract

Objectives
T peripheral helper (Tph) cells have major roles in pathological processes in SLE. We sought to clarify the mechanisms of Tph cell differentiation and their relevance to clinical features in patients with SLE.

Method
Phenotypes and functions of Tph cell-related markers in human CD4+ T cells purified from volunteers or patients were analysed using flow cytometry and quantitative PCR. Renal biopsy specimens from patients with LN were probed by multicolour immunofluorescence staining.

Results
Among multiple cytokines, transforming growth factor (TGF)-β3 characteristically induced programmed cell death protein 1 (PD-1)hi musculoaponeurotic fibrosarcoma (MAF)+, IL-21+IL-10+ Tph-like cells with a marked upregulation of related genes including PDCD-1, MAF, SOX4 and CXCL13. The induction of Tph-like cells by TGF-β3 was suppressed by the neutralization of TGF-β type II receptor (TGF-βR2). TGF-β3-induced Tph-like cells efficiently promoted the differentiation of class-switch memory B cells into plasmocytes, resulting in enhanced antibody production. The proportion of Tph cells in the peripheral blood was significantly increased in patients with SLE than in healthy volunteers in concordance with disease activity and severity of organ manifestations such as LN. TGF-β3 was strongly expressed on macrophages, which was associated with the accumulation of CD4+ C-X-C chemokine receptor (CXCR5)-PD-1+ Tph cells, in the renal tissue of patients with active LN.

Conclusion
The induction of Tph-like cells by TGF-β3 mainly produced from tissue macrophages plays a pivotal role in the pathological processes of active LN by enhancing B-cell differentiation in patients with SLE.
Topical Ophthalmic Anesthetic Use for Sterile Corneal Abrasion in the Emergency Department
Authors: Black Alexander , Boswell Kathryn , Montenegro Daniel 
Published: November 2022

Objectives:
Investigate current treatment and prescription practices of topical ophthalmic anesthetic agents (TOAAs) in emergency departments (EDs) across the United States to understand how these encounters may affect ocular health.

Methods:
A six question survey regarding the use of topical ophthalmic anesthetics was completed by 73 emergency medicine (EM) physicians across 34 states through the American College of Emergency Physicians (ACEP) Emergency Medicine Practices Research Network (EMPRN).

Results:
The survey was completed by 73 EM physicians. The majority indicated treating eye-related complaints frequently and comfort using (TOAAs) for pain control in sterile corneal abrasion. Over half of respondents indicated never discharging a patient from the ED with a prescription for TOAAs. The consensus for patient instructions included offering a short course (<48 hr) of treatment. Furthermore, 96% of respondents indicated that they would not prescribe refills for TOAAs, mostly due to concern regarding corneal damage.

Conclusions:
Despite demonstrable concern regarding the use of TOAAs and risk of corneal damage, a minority of EM physicians continue to prescribe these agents in the treatment of sterile corneal abrasion, which poses risks to visual health. Ophthalmologists have begun to advocate for decreased availability of TOAAs in settings such as the ED.
Application of microfluidic chip technology to study the inhibitory effect of tetramethylpyrazine on platelet aggregation, activation, and phosphatidylserine exposure mediated by pathological high shear rate
Authors: Zhang Tiancong , Liu Ling , Huang Xiaojing , Gao Xuemei , Chen Dan , Huan Xuanrong , He Cui , Li Yuan 
Published: November 2022

Objective
In order to study the antithrombotic effect and mechanism of tetramethylpyrazine (TMA).

Methods
In this study, we developed a microfluidic chip model that can mimic normal arteries and stenotic arterial vessels, and studied the inhibitory effects of TMA on platelet aggregation, activation (P-selectin, GPIIb/IIIa, monocyte-platelet aggregates) and phosphatidyl serine (PS) exposure. In addition, we also investigated the effect of TMA on ADP and ristocetin-induced platelet aggregation by turbidimetry.

Results
The results showed that TMA significantly inhibited the platelet aggregation, activation and PS exposure induced by pathological high shear rate. Under static conditions, TMA can inhibit ADP and ristocetin-induced platelet aggregation.

Conclusion
The results indicated that TMA mainly inhibited platelet aggregation, activation and PS exposure by inhibiting the binding of von Willebrand factor (vWF) to the GPIb/IX/V complex, and partially inhibited platelet aggregation through the platelet P2Y12–ADP receptor pathway.
Early user experience and lessons learned using ultra-portable digital X-ray with computer-aided detection (DXR-CAD) products: A qualitative study from the perspective of healthcare providers
Authors: Qin Zhi Zhen , Barrett Rachael , del Mar Castro Maria , Zaidi Sarah , Codlin Andrew J. , Creswell Jacob , Denkinger Claudia M. 
Published: November 2022

AbstractBackgroundRecent technological and radiological advances have renewed interest in using X-rays to screen and triage people with tuberculosis (TB). The miniaturization of digital X-ray (DXR), combined with automatic interpretation using computer-aided detection (CAD) software can extend the reach of DXR screening interventions for TB. This qualitative study assessed early implementers’ experiences and lessons learned when using ultra-portable (UP) DXR systems integrated with CAD software to screen and triage TB.MethodsSemi-structured interviews were conducted with project staff and healthcare workers at six pilot sites. Transcripts were coded and analyzed using a framework approach. The themes that emerged were subsequently organized and presented using the Consolidated Framework for Implementation Research (CFIR).ResultsThere were 26 interviewees with varying roles: supervisory, clinicians, radiographers, and radiologists. Participants recognized the portability as the main advantage, but criticize that it involves several compromises on throughput, internet dependence, manoeuvrability and stability, as well as suitability for patients with larger body sizes. Furthermore, compared to use the hardware and software from the same supplier without digital health information system, complexity increases with interoperability between hardware and software and between different electronic health information systems. Currently, there is a limited capacity to implement these technologies, especially due to need for threshold selection, and lack of guidance on radiation protection suitable for UP DXR machines. Finally, the respondents stressed the importance of having protected means of sharing patient medical data, as well as comprehensive support and warranty plans.ConclusionStudy findings suggest that UP DXR with CAD were overall well received to decentralize radiological assessment for TB, however, the improved portability involved programmatic compromises. Main barriers to the uptake included the insufficient capacity and lack of guidance on radiation protection suitable for UP DXR.
Cost-effectiveness of short, oral treatment regimens for rifampicin resistant tuberculosis
Authors: Sweeney Sedona , Berry Catherine , Kazounis Emil , Motta Ilaria , Vassall Anna , Dodd Matthew , Fielding Katherine , Nyang’wa Bern-Thomas 
Published: November 2022

AbstractIntroductionCurrent options for treating tuberculosis (TB) that is resistant to rifampicin (RR-TB) are few, and regimens are often long and poorly tolerated. Following recent evidence from the TB-PRACTECAL trial countries are considering programmatic uptake of 6-month, all-oral treatment regimens.MethodsWe used a Markov model to estimate the incremental cost-effectiveness of three regimens containing bedaquiline, pretomanid and linezolid (BPaL) with and without moxifloxacin (BPaLM) or clofazimine (BPaLC) compared with the current mix of long and short standard of care (SOC) regimens to treat RR-TB from the provider perspective in India, Georgia, Philippines, and South Africa. We estimated total costs (2019 USD) and disability-adjusted life years (DALYs) over a 20-year time horizon. Costs and DALYs were discounted at 3% in the base case. Parameter uncertainty was tested with univariate and probabilistic sensitivity analysis.ResultsWe found that all three regimens would improve health outcomes and reduce costs compared with the current programmatic mix of long and short SOC regimens in all four countries. BPaL was the most cost-saving regimen in all countries, saving $112-$1,173 per person. BPaLM was the preferred regimen at a willingness to pay per DALY of 0.5 GDP per capita in all settings.ConclusionsOur findings indicate BPaL-based regimens are likely to be cost-saving and more effective than the current standard of care in a range of settings. Countries should consider programmatic uptake of BPaL-based regimens.
Drought effects of annual and long-term temperature and precipitation on mortality risk for 9 common European tree species
Authors: Neumair Matthias , Ankerst Donna P. , Potočić Nenad , Timmermann Volkmar , Ognjenović Mladen , Brandl Susanne , Falk Wolfgang 
Published: November 2022

AbstractRisk factors for natural tree mortality in managed forests, excluding wind and snow induced breakage, fires and thinning, can be difficult to identify due to correlated confounders of long- and short-term weather patterns with tree age. This study quantified the association of annual and long-term 30-year average temperature and precipitation effects on individual tree death across Europe from 2011 to 2020 for European beech, sessile and pedunculate oak, silver birch, black pine, Austrian oak, Scots pine, European hornbeam, and Norway spruce. For each species, logistic regression approaches for predicting annual mortality evaluated the influence of age, exposition and weather effects on individual tree death, while accounting for multi-collinearity of risk factors. For all species except sessile oak, higher 30-year-temperature averages were associated with higher odds of tree mortality. Effect size of other risk factors varied among species, with similar weather associations between Austrian and sessile oak on the one hand, and Scots pine, Norway spruce and pedunculate oak on the other hand. In particular, warmer winters reduced mortality for silver birch, sessile and Austrian oaks, while having the opposite association for the other species. Sessile oak was most robust against drought effects and could serve as an important tree species under climate change scenarios.
A potential function for the helicase Dbp5 in cytoplasmic quality control
Authors: Querl Luisa , Lu Yen-Yun , Beißel Christian , Krebber Heike 
Published: November 2022

ABSTRACTAccurate translation requires correct mRNAs with intact open reading frames. Cells eliminate defective transcripts to prevent mistranslation by three cytoplasmic mRNA quality control events termed nonsense-mediated decay (NMD), no-go decay (NGD) and non-stop decay (NSD). Translation termination on correct transcripts requires Dbp5 (human DDX19), which delivers eRF1 to the ribosomes and prevents an early contact of eRF1 with eRF3, precluding the immediate dissociation of both release factors and subsequent termination readthrough. Here, we report evidence for an influence of Dbp5 on NMD, as it delivers eRF1 also to PTC-containing transcripts. In contrast to regular translation termination and NMD, functional NGD and NSD require the eRF1-eRF3-like proteins Dom34-Hbs1. We suggest that Dbp5 delivers Dom34 to NGD and NSD substrates as well. However, in contrast to regular termination, it does not prevent an Hbs1 contact, but allows formation of a ternary Dom34-Hbs1-Dbp5 complex. The Dbp5-mediated delivery of Dom34-Hbs1 in NGD and NSD might rather shield and position the complex to prevent a premature contact of Dom34 and Rli1 to prevent inefficient splitting of the ribosomal subunits. Together, we have gathered evidence suggesting an important role of Dbp5 in cytoplasmic mRNA quality control.