bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sleep Deprivation among Health-Care Workers: A Cross-sectional Study at the University of Gondar Comprehensive Specialized Hospital
Authors: Getu Ayechew Adera , Tesfaye Winta , Yeshaw Yigizie , Dagnew Baye , Addis Yohannes 
Published: April 2022

Abstract
Background: Sleep disturbance is a common problem among health care workers and it has been associated with poor clinical services including medical errors. Objective: This study aimed to determine the prevalence of sleep deprivation and identify factors impacting among health care workers at the University of Gondar Comprehensive Specialized Hospital, Gondar, Ethiopia. Methods: Health care workers were approached to subjectively assessed their sleep quality. Study participants completed the Pittsburgh Sleep Quality Index (PSQI), and they reported demographic, working departments, and behavioral information. Poor sleep quality was defined as a PSQI score >5. Multiple binary logistic regression was used to estimate the strength of the association between risk factors and sleep deprivations. P value ≤ 0.05 was used to declare statistically significant. Results: A total of 418 surveys were completed. The mean age of the study participants was 30.7 years (SD ± 6.3). The overall prevalence of poor sleepers was 58.9% [95% CI (54.2, 63.6%)]. Being female [AOR=1.9, 95%CI (1.2, 2.9)], working in night shifts [AOR=5.7, 95%CI (2.3, 14.3), physically inactive [AOR=2.08(1.2-3.6)], chewing khat [AOR = 3.1, 95% CI (1.2, 7.6)], and the presence of depressive symptoms [AOR = 2.6, 95% CI (1.3, 6.8)] were significantly associated with sleep deprivation. Conclusion: Based on the PSQI scoring, the prevalence of sleep deprivation among health care workers was high. Night shiftwork schedule, lack of regular physical activity, chewing khat, and the presence of depressive symptoms were associated with sleep deprivation. This research suggests sleep interventions for health care workers.
Two-body freefall time in a matter dominant expanding universe
Authors: Xu Sean 
Published: April 2022

Abstract
Predicting freefall time is a major problem in galactic dynamics. The relaxation time of collisionless dark matter, which heavily influences galaxy evolution, is closely related to its freefall time. Calculating freefall time using Kepler’s Third Law does not consider the effects of the universe’s expansion. This paper proposes a new formula for calculating freefall time in an expanding background. The derivation of the formula starts from the governing equations of an N-body system in an expanding background. Two-body simulations were run using equations of motion with constant damping (to represent the effects of expansion) derived from the N-body equations. More than 5,200 different two-body systems with different separation distances, mass, and damping were simulated. The simulated freefall time was compared with good agreement against the proposed formula. The results demonstrate that two-body freefall time is dependent on the time at which collapse began. The earlier it began, the longer the freefall time. A weak gravitational attraction, caused by the small mass of the system or the great distances involved, exacerbates this effect. The galaxy that started to form earlier is expected to take longer to form. A better understanding of freefall time can lead to greater insight into galaxies’ historical and future evolution.
Intraoperative abobotulinumtoxinA alleviates pain after surgery and improves general wellness in a translational animal model
Authors: Cornet Sylvie , Carré Denis , Limana Lorenzo , Castel David , Meilin Sigal , Horn Ron , Pons Laurent , Evans Steven , Lezmi Stephane , Kalinichev Mikhail 
Published: April 2022

Abstract
Pain after surgery remains a significant healthcare challenge. Here, activity of abobotulinumtoxinA (aboBoNT-A, Dysport®) was assessed in a post-surgical pain model in pigs. Full-skin-muscle incision and retraction surgery on the lower flank was followed by intradermal injections of either aboBoNT-A (100, 200, or 400 U/pig), vehicle (saline), or wound infiltration of liposomal extended-release bupivacaine. Animals were assessed for mechanical sensitivity, distress behaviors, latency to approach the investigator for 5 days following surgery. Immunohistochemical analyses of total and cleaved synaptosomal-associated protein-25 kD (SNAP-25) and pain-related biomarkers were performed in the aboBoNT-A-400 U and saline groups. At Day 1, partial reversion of mechanical allodynia was achieved in aboBoNT-A groups with full reversion from Day 3. Reduced distress and normalized approaching responses were observed with aboBoNT-A from 6 h post-surgery. Bupivacaine reversed mechanical allodynia for 24 h after surgery but did not affect distress or approaching responses. Cleaved SNAP-25 was absent in the skin and dorsal root ganglia but present in the ipsilateral dorsal horn of aboBoNT-A-injected animals. A decrease in some pain-related biomarkers was seen with aboBoNT-A versus saline. Clinical investigation to support the use of aboBoNT-A as a tolerable and efficacious analgesic drug for post-surgical pain, is warranted.
Cortisol Response to Psychosocial Stress in Coronary Artery Disease Patients: The Role of Mental Distress, Fatigue and Quality of Life
Authors: Gecaite-Stonciene Julija , Hughes Brian M. , Kazukauskiene Nijole , Bunevicius Adomas , Burkauskas Julius , Neverauskas Julius , Bellani Marcella , Mickuviene Narseta 
Published: April 2022

Abstract
We aimed to explore the relationship between cortisol response to psychosocial stress, mental distress, fatigue and HRQoL in individuals with coronary artery disease (CAD) after recent acute coronary syndrome (ACS). A cross-sectional study initially included 113 subjects (88% men, 53 ± 7 years) 1–3 weeks after ACS. Cortisol response was assessed by measuring salivary cortisol during Trier Social Stress Test. Mental distress was measured with Hospital Anxiety and Depression Scale, State-Trait Anxiety Inventory, and Type D Scale-14. Fatigue symptoms were evaluated using Multidimensional Fatigue Inventory 20-items, while Health related quality of life (HRQoL) was assessed with 36-Item Short Form Medical Outcome Questionnaire. After conducting multivariable linear regression analyses, diminished cortisol response during public speech was significantly associated with higher anxiety symptoms (β=-0.224; p = 0.035), while diminished cortisol response during pre-task preparation was significantly linked with the presence of Type D personality (β=-0.290; p = 0.006; β=-0.282; p = 0.008 respectively), even after controlling for confounders (i.e., sex, age, education, NYHA functional class, beta-blockers and baseline levels of cortisol measures). We found that mental distress, but not fatigue and HRQoL, was linked with blunted cortisol response during psychosocial stress, independently of potential covariates.
In silico identification of the rare-coding pathogenic mutations and structural modeling of human NNAT gene associated with Anorexia Nervosa
Authors: Azmi Muhammad Bilal , Naeem Unaiza , Saleem Arisha , Jawed Areesha , Usman Haroon , Qureshi Shamim Akhter , Azim Muhammad Kamran 
Published: April 2022

Abstract
Purpose Increase susceptibility towards Anorexia nervosa (AN) was reported with reduced levels of NNAT gene. We sought to investigate the most pathogenic rare-coding missense mutations (nsSNPs) of NNAT and their potential damaging impact on protein function through transcript level sequence and structure based in silico approaches. Methods Gene sequence, SNPs of NNAT was retrieved from public databases and the putative post-translational modification (PTM) sites were analyzed. Distinctive in silico algorithms were recruited for transcript level SNPs analyses and to characterized high risk rare-coding nsSNPs along with their impact on protein stability function. Ab initio 3D-modeling of wild-type, alternate model prediction for most deleterious nsSNP, validation and recognition of druggable binding pockets were also performed. AN 3D therapeutic compounds followed rule of drug-likeness were docked with most pathogenic variant of NNAT to estimate the drugs’ binding free energies. Results Conclusively, 10 transcripts (201–205) based nsSNPs from 3 rare-coding missense variants i.e., rs539681368, rs542858994, rs560845323 out of 840 exonic SNPs were identified. Transcript based functional impact analyses predicted rs539681368 (C30Y) from NNAT-204 as the high risk rare-coding pathogenic nsSNP, deviating protein functions. The 3D-modeling analysis of AN drugs’ binding energies indicated lowest binding free energy (ΔG) and significant inhibition constant (Ki) with mutant models C30Y. Conclusions Mutant model (C30Y) exhibiting significant drug binding affinity and the commonest interaction observed at the acetylation site K59. Thus, based on these findings, we concluded that the identified nsSNP may serve as potential targets for various studies, diagnosis and therapeutic interventions. Level of evidence No level of evidence
Placental macrophage (Hofbauer cell) response to viral and bacterial ligands is influenced by fetal sex
Authors: Pantazi Paschalia , Kaforou Myrsini , Tang Zhonghua , Abrahams Vikki , McArdle Andrew , Guller Seth , Holder Beth 
Published: April 2022

Abstract
Bacterial and viral infections of the placenta are associated with inflammation and adverse pregnancy outcomes. Hofbauer cells (HBCs) are specialised fetal-origin macrophages in the placental villi and are proposed to protect the fetus from vertical transmission of pathogens; however, they are poorly understood. Here, we have performed quantitative proteomics on term HBCs under resting conditions and following exposure to bacterial and viral pathogen associated molecular patterns (PAMPs), and investigated the contribution of fetal sex to these responses. Resting HBCs expressed a plethora of proteins pertinent to macrophage function, including chemokines, cytokines, Toll-like receptors, and classical and non-classical major histocompatibility complex class I and II molecules. HBCs mounted divergent responses to bacterial versus viral PAMPs but exhibited protein expression changes suggestive of a switch towards a more pro-inflammatory phenotype. A comparison between male and female HBCs, showed that the latter mounted a much stronger and wider response. Sexual dimorphism in HBCs was primarily associated with lipid metabolism in males and cytoskeleton organisation in females. We provide a novel and comprehensive understanding regarding the phenotype of term placental macrophages and their sex-dependent responses to infectious triggers.
MiRNA-6870-3p regulates LPS-induced epicardial adipose tissue inflammatory genes via targeting Tollip-mediated JNK and NF-κB signaling in coronary artery disease
Authors: Wu JianGuo , Yang wenkai , Huang haozhong , Tang kai , Zheng Guang-Di 
Published: April 2022

Abstract
Objective: MiR-6870-3p acts as a crucial regulator of gene expression at the posttranscriptional level and participates in immune responses. However, the roles of miR-6870-3p and its target genes and their underlying mechanisms in the inflammatory responses of epicardial adipose tissues (EATs) are unknown.Methods: MiRNA microarray was used to collect miRNA expression profiles in EATs from five patients with coronary artery disease (CAD) and four individuals without CAD (n-CAD). Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to check the expression of miR-6870-3p in CAD and n-CAD EATs. The mRNA and protein expression levels of Tollip and the key genes of the TLR4 signaling pathway were examined by qRT-PCR and Western blot. The levels of inflammatory factors in the cell supernatant were measured by enzyme-linked immunosorbent assay. Bioinformatics algorithms were used to predict the target gene of miR-6870-3p, and the result was validated by dual-luciferase reporter assay. The protein expression levels of JNK and NF-κB were measured by Western blot.Resules:Our results showed that miR-6870-3p was remarkably higher in CAD EATs than in n-CAD EATs. MiR-6870-3p was positively correlated with TLR4, IL-6, JNK, NF-κB (p65), and TNF-α in CAD EAT samples. Lipopolysaccharide (LPS) treatment upregulated miR-6870-3p mRNA expression and downregulated Tollip mRNA and protein expression in macrophages. MiR-6870-3p upregulation also aggravated the production of proinflammatory cytokines, such as TNF-α and IL-6, when the macrophages were stimulated with LPS. The result of luciferase reporter assays confirmed that miR-6870-3p directly targets Tollip. Moreover, miR-6870-3p upregulation in macrophages resulted in the activation of the JNK/NF-κB pathway.Conclusion:Our study concluded that miR-6870-3p regulates human EAT inflammation by targeting Tollip-mediated JNK and NF-κB signaling events.
Revealing the Evolution of the Tumor Immune Microenvironment in Follicular Lymphoma Patients Progressing Within 24 Months Using Single-cell Imaging Mass Cytometry
Authors: Liu Long , Yu Xingxing , Li Zhifeng , He Xiaohua , Zha Jie , Lin Zhijuan , Hong Yan , Zheng Huijian , Lai Qian , Ding Kaiyang , Jia Xian , Fu Guo , Yu Haifeng , Yang Haiyan , Li Zhiming , Young Ken H. , Xu Bing 
Published: April 2022

Abstract
Background: The tumor immune microenvironment (TIME) is crucial for follicular lymphoma (FL) pathogenesis and progression. However, TIME evolution during progression of disease within 24 months (POD24) is unknown.Methods: Spatially-resolved and single-cell image mass cytometry with a panel of 36 meta-tagged antibodies was quantitatively used to dissect the TIME structure in 13 paired FLs at diagnosis and POD24. Results: Increased CD163− macrophages with PD-1 ligand upregulation and decreased CD8+T cells with upregulated LAG3 expression were observed in the follicles during POD24. Spatial interactions demonstrated that FL cells interacted more intimately with macrophages rather than Tregs and less with cytotoxic cells during POD24. Additionally, more CD8+T cells near FL cells interacted with macrophages but not Tregs during POD24. Notably, macrophages also cooperated with Tregs more frequently to simultaneously hijack the cytotoxic T-cells and protect FL cells, subsequently generating a more immunosuppressive environment in the follicles during POD24. Conclusions: FL cells reside in a more immune-compromised microenvironment, evading immune-cell attacks during POD24. Novel immunotherapeutic approaches harnessing LAG3, macrophages, and Tregs will be promising to overcome the poor outcomes of patients with FL POD24.
Whole-genome comparison of representatives of all variants of SARS-CoV-2, including subvariant BA.2 and the GKA clade
Authors: Suardana Ida Bagus Kade , Mahardika Bayu K. , Pharmawati Made , Sudipa Putu H. , Sari Tri Komala , Mahendra Nyoman B. , Mahardika Gusti N. 
Published: April 2022

Abstract
Since its discovery at the end of 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly evolved into many variants, including subvariant BA.2 and the GKA clade. Genomic clarification is needed for better management of the current pandemic as well as the possible re-emergence of novel variants. The sequence of the original strain Wuhan-Hu-1 and approximately 20 representatives of each variant were downloaded from GenBank and GISAID. Two representatives with no track of un-definitive nucleotides were selected. The sequences were aligned using Muscle. The location of insertion/deletion (indel) in the genome was mapped following the open reading frame (ORF) of Wuhan-Hu-1. Amino acid substitutions in all ORFs were analysed separately. Evolutionary analysis was inferred using maximum likelihood. There are two indel sites in ORF1AB, eight in spike, and one each in ORF3A, Matrix (MA), Nucleoprotein (NP), and the 3’-untranslated regions (3’UTR). Some indel sites are not unique, and some are variant specific. There are 10 residues shared by the Omicron, BA2, and GKA lineages. Three deletions in NP are unique to Omicron and BA.2; two substitutions in ORF1AB, four in spike, three in NP, and two in ORF7A and ORF8 were exclusive to the Delta and GPA clades. Delta, Omicron, and BA.2 share the same single residue spike. Three insertions in spike are unique for Omicron and GKA. Phylogenetic analysis shows that the Omicron, BA.2, and GKA clades share a common cluster that emerged from Delta. In conclusion, whole-genome comparison reveals indels and polymorphic amino acids specific to variants or sub-variants. We propose that the GKA clade is an Omicron subvariant. Finally, the higher transmissibility of BA.2 might be attributed to a 48-nucleotide deletion in the 3’UTR.
Management in Business Analytics: An Ego-Centric NetworkApproach
Authors: C Correa Juan , Lukas Arango , Hugo Díaz , Luis Zárate , Karim Kure , Carlos Huertas , Nicolas Gomez 
Published: April 2022

Abstract
Managers know well the utility of business analytics for decision-making purposes.Nonetheless, quantitative scrutiny on the link between management and businessanalytics remains unexplored. We address this gap by combining bibliometric datacollection techniques with text mining and ego-centric network analyses followingreproducible research standards. Results show that only 12.33% of a total of 381sampled documents from the Scopus database revealed an explicit link between theterm “management” with the bigram “business analytics” and the size the ego-centric network proved to be almost a quarter of the size of the extensive networkthat represents all conceptual keyword co-occurrences in the literature. We arguethat, in contrast to common wisdom, our results suggest that the link betweenbusiness analytics and management remains incipient, and this has essential teach-ing implications for business schools aiming at including business analytics in theircurricula.
Clinical determinants and prognostic significance of hypocapnia in acute heart failure.
Authors: Garus Mateusz , Zdanowicz Agata , Fudim Marat , Zymliński Robert , Niewiński Piotr , Paleczny Bartłomiej , Rosiek-Biegus Marta , Iwanek Gracjan , Ponikowski Piotr , Biegus Jan 
Published: April 2022

Abstract
Aim.
The aim of this research was to examine the prevalence of hyperventilation (defined by pCO2 value) among acute heart failure (AHF) patients and to link it with potential triggers and prognosis.
Methods.
AHF patients underwent multidirectional assessment including capillary blood gas examination on hospital admission and during hospitalization.
Results.
Out of 241 AHF patients, 57(24%) were assigned to low pCO2 group (pCO2 > 30mmHg) and 184 (76%) to normal pCO2 group (pCO2 > 30mmHg). Low pCO2 group had significantly lower HCO3- (22.3 ± 3.4 mmol/L vs 24.7 ± 2.9 mmol/L p < 0.0001) and significantly higher lactate level (2.53 ± 1.6 vs 2.14 ± 0.97 p = 0.03). No differences between groups were observed in respect to the following potential triggers of hyperventilation: hypoxia (sO2 92.5 ± 5.2 vs 92 ± 5.6, p = 0.57), infection (CRP 10.5[4.9–26.4] vs 7.15[3.45–17.35], p = 0.47), dyspnea severity (7.8 ± 2.3 vs 8.0 ± 2.3, p = 0.59) and pulmonary congestion (82.5% vs 89.1%, p = 0.19), respectively. Low pCO2 value was related to an increased one-year all-cause mortality hazard ratio (HR) (95%CI): 2.2 (1.3–3.6); p = 0.002 and risk of death and of rehospitalization for HF, HR (95%CI): 2.0 (1.3-3.0); p = 0.002.
Conclusion.
Hyperventilation is relatively frequent in AHF and is related to poor prognosis. Low pCO2 was not contingent on expected potential triggers of dyspnea but rather on tissue hypoperfusion.
Potentially Harmful Metals, Water Toxicity, Source Apportionment, and Potential Health Risk in Groundwater of Sheikhupura, Pakistan
Authors: Ullah Zahid , Ghani Junaid , Rashid Abdur , Ali Asmat , Iqbal Javed , Rehman Hamad Ur 
Published: April 2022

Abstract
The presence of potentially harmful metals (PHMs) in drinking groundwater is a common environmental problem in Pakistan. Ingestion of PHMs polluted water, can cause a variety of harmful health impacts in humans. However, in Pakistan, only a little level of attention is given to quantitative and qualitative evaluation of groundwater utilized for ingestion motive. Therefore, this research was aimed for the first time to evaluate the levels of PHMs in drinking groundwater sources of Sheikhupura, Pakistan. A total of (n=243) samples were collected and subjected to analyze PHMs concentration like arsenic (As), manganese (Mn), lead (Pb), zinc (Zn), copper (Cu), nickel (Ni), iron (Fe), along with pH, total dissolved solids (TDS), and electrical conductivity (EC). The results show the concentration of PHMs such as As (91%), Mn (14%), Pb (97%), Fe (45%), Zn (15%), Cu (0%), and Ni (0%), samples were beyond the WHO recommended values for PHMs in drinking water. Saturation indices results shows that the aquifers of the study area are saturated for lead hydroxide, zinc hydroxide, and goethite minerals, indicating that these minerals have a vital role in the contamination of groundwater sources of the study area. Principal component analysis (PCA) results with total variability of (60.154%) reveal that the groundwater sources of the study area are contaminated due to (30.9%) geogenic sources, (31.3%) anthropogenic sources, and (37.6%) of mixed type including both geogenic and anthropogenic sources. Health risk exposure assessment results predicted potential health risk on the population of the study area.
The complete chloroplast genome sequence of an Endangered orchid species Dendrobium huoshanense (Orchidaceae) and phylogenetic analysis with related species
Authors: Wang Bin , Chen Jun , Li Min 
Published: April 2022

Abstract
The family Orchidaceae, including many medicinal plants, is a famous ornamental plant. While, Dendrobium huoshanense is an endangered Orchidaceae species mainly distributed in Huoshan county, Anhui province, China. Here, using the program MITObim v1.7, we assembled the complete chloroplast genome sequence of D. huoshanense, which consists of 153,188 bp, and a pair of inverted repeats (IR) of 26,525 bp. The genome also includes one small and one large single-copy region (SSC and LSC) of 14,524 bp and 85,614 bp, respectively. GC content of the genome is 40.0%, and it contains 129 genes coding for 76 proteins. Codon usage analysis showed that a total of 23,448 codons were identified, and the simple-sequence repeat sequences mainly contain A/T mononucleotide. Phylogenetic analyses revealed that D. huoshanense belongs to the Dendrobium of the Orchidaceae family, which is in accordance with the traditional classification. The research finding in this paper will be beneficial for further investigations on Dendrobium huoshanense from the aspect of evolution, and chloroplast genetic engineering.