bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preventable risk factors for type 2 diabetes can be detected using noninvasive spontaneous electroretinogram signals
Authors: Imm Ramsés Noguez , Muñoz-Benitez Julio , Medina Diego , Barcenas Everardo , Molero-Castillo Guillermo , Reyes-Ortega Pamela , Hughes-Cano Jorge Armando , Medrano-Gracia Leticia , Miranda-Anaya Manuel , Rojas-Piloni Gerardo , Quiroz-Mercado Hugo , Hernández-Zimbrón Luis Fernando , Fajardo-Cruz Elisa Denisse , Ferreyra-Severo Ezequiel , García-Franco Renata , Mijangos Juan Fernando Rubio , López-Star Ellery , García-Roa Marlon , Lansingh Van Charles , Thébault Stéphanie C. 
Published: November 2022

Abstract
Given the ever-increasing prevalence of type 2 diabetes and obesity, the pressure on global healthcare is expected to be colossal, especially in terms of blindness. Electroretinogram (ERG) has long been perceived as a first-use technique for diagnosing eye diseases, and some studies suggested its use for preventable risk factors of type 2 diabetes and thereby diabetic retinopathy (DR). Here, we show that in a non-evoked mode, ERG signals contain spontaneous oscillations that predict disease cases in rodent models of obesity and in people with overweight, obesity, and metabolic syndrome but not yet diabetes, using one single random forest-based model. Classification performance was both internally and externally validated, and correlation analysis showed that the spontaneous oscillations of the non-evoked ERG are altered before oscillatory potentials, which are the current gold-standard for early DR. Principal component and discriminant analysis suggested that the slow frequency (0.4–0.7 Hz) components are the main discriminators for our predictive model. In addition, we established that the optimal conditions to record these informative signals, are 5-minute duration recordings under daylight conditions, using any ERG sensors, including ones working with portative, non-mydriatic devices. Our study provides an early warning system with promising applications for prevention, monitoring and even the development of new therapies against type 2 diabetes.
Novel nanocellulose - microcrystalline cellulose complex with hierarchical structure reinforcing polylactic acid as 3D printing materials
Authors: Long Haibo , Mo Jianbin , Yang Feiwen , Xiao Jialin , Zhang Shuting , Xao Dingshu , Guan Litao , Zheng Wenxu , Gu Jin , Zhou Wuyi , Wei Yen , Dong Xianming 
Published: November 2022

Abstract
A novel nanocellulose - microcrystalline cellulose complex (NCMC) with special hierarchical structure has been prepared by grafting nanocellulose (NC) onto the surface of microcrystalline cellulose (MCC) via different silane coupling agents in this paper. The cellulose complex was easier to separate and purify from the solution in the preparation process than nanocellulose due to its large size, and was used to reinforce polylactic acid (PLA) as 3D printing materials through a melt-blending process. The results showed that NCMCs prepared by different silane coupling agents had different surface morphologies. Nanocelluloses formed a network structure on the surface of KH-550 treated NCMC, while a spherical structure wrapped by nanocellulose was formed on the surface of KH-560 treated NCMC, and KH-570 treated NCMC was the nanocellulose network with a small amount of irregular silica impurities. The mechanical properties, crystallinity of polymer and thermal stability of NCMC/PLA composites are obviously improved by adding a small amount of NCMC. Compared to pure PLA, the tensile strength, bending strength and elongation at break of the NCMC/PLA composites were increased by 40.3%, 84.1% and 34.3%, respectively. It is because NCMC had higher specific surface area and more complex surface structure than MCC, that led to more thorough grafting reaction on polylactic acid during high temperature extrusion blending process. The DSC results also showed that a small amount of NCMC could promote the crystallization of PLA as 3D printing materials. Besides, NCMC/PLA composites did not block the printing nozzle in continuous 3D printing, and the printed products had better appearances. The obtained materials are environmentally friendly, renewable and ideal for 3D printing.
High intestinal vascular permeability in a murine model for Hirschsprung’s disease; implications for postoperative Hirschsprung-associated enterocolitis
Authors: Suda Kazuto , Yamada Shunsuke , Miyahara Katsumi , Fujiwara Naho , Kosaka Seitaro , Abe Kumpei , Seo Shogo , Nakamura Shinji , Lane Geoffrey J. , Yamataka Atsuyuki 
Published: November 2022

Abstract
Purpose
Intestinal vascular permeability (VP) in a murine model for Hirschsprung’s disease (HD) and postoperative Hirschsprung-associated enterocolitis (HAEC) were investigated.
Methods
Intestinal VP was determined using a Miles assay using 1% Evans blue injected into a superficial temporal vein of newborn endothelin receptor-B KO HD model (KO) and syngeneic wild-type (WT) mice (n=5, respectively). Extravasated Evans blue in normoganglionic ileum (Ng-I), normoganglionic proximal colon (Ng-PC) and aganglionic distal colon (Ag-DC) was quantified by absorbance at 620nm. Quantitative polymerase chain reaction (qPCR) for Vascular Endothelial Growth Factor A (VEGF-A), VEGF-B, CDH5, SELE and CD31, and immunofluorescence for CD31 were performed.
Results
VP was significantly higher in Ng-I, Ng-PC, and Ag-DC from KO than WT (p<0.01, p<0.05, and p<0.05, respectively). qPCR demonstrated upregulated VEGF-A in Ng-I and Ag-DC, VEGF-B in Ng-I, and SELE in Ng-I and Ng-PC (p<0.05, p<0.05, p<0.05, p<0.01 and p<0.05, respectively), and downregulated CDH5 in Ng-I and Ng-PC from KO (p<0.05, respectively). Expression of CD31 mRNA in Ng-I and Ag-DC from KO was significantly higher on qPCR (p<0.05) but differences on immunofluorescence were not significant.
Conclusions
VP may be etiologic for postoperative HAEC throughout the intestinal tract even after excision of aganglionic bowel.
A protocol for a randomised controlled trial exploring human milk, nutrition, growth, and breastfeeding rates at discharge: the Hummingbird study
Authors: Chmelova Kristina , Berrington Janet , Shenker Natalie , Zalewski Stefan , Rankin Judith , Embleton Nicholas 
Published: November 2022

Abstract
Background
Mother’s own breast milk (MOM) is the optimal nutrition for preterm infants as it reduces the incidence of key neonatal morbidities and improves long-term outcomes. However, MOM shortfall is common and either preterm formula (PF) or pasteurised donor human milk (DHM) may be used, although practice varies widely. Limited data suggest that the use of DHM may impact on maternal beliefs and behaviours and may therefore impact on breastfeeding rates. The aim of this pilot study is to determine if the duration of DHM exposure impacts on breastfeeding rates, and maternal breastfeeding self-efficacy.
Methods
The Human Milk, Nutrition, Growth, and Breastfeeding Rates at Discharge (Hummingbird) study is a feasibility and pilot, non-blinded, randomised controlled trial (RCT) with a contemporaneous qualitative evaluation. Babies born at less than 33 weeks gestation or with birth weight <1500 grams whose mothers intend to provide MOM are randomly assigned to either control arm (DHM used to make up shortfall until full feeds, then PF used thereafter) or intervention arm (DHM used to make up shortfall until 36 weeks corrected age or discharge if sooner). The primary outcome is breastfeeding rates at discharge. Secondary outcomes include growth, key neonatal morbidities, length of stay, breastfeeding self-efficacy and postnatal depression using validated questionnaires. Qualitative interviews using a topic guide will explore perceptions around use of DHM and will be analysed using thematic analysis.
Discussion
The provision of DHM is a complex intervention as it impacts on infant health and disease, as well as impacting on maternal and healthcare staff belief and behaviour. This is the first RCT to investigate the impact of DHM duration on breastfeeding rates and maternal self-efficacy.
Trial registration: Trial was registered prospectively on 4th May 2021 (ISRCTN 57339063).
Phytoremediation: An effective technique for domestic wastewater treatment in developing countries
Authors: Harne Kailash , Joshi Himanshu 
Published: November 2022

Abstract
Phytoremediation in the form of constructed wetlands is an effective method of treatment of municipal as well as industrial wastewater. The efficacy of this method has already been tested in European and American countries and found to be the best method of wastewater treatment. Efforts have been made in the present work to check the efficacy of phytoremediation in the form of constructed wetlands under Indian climatic conditions. A pilot plant of constructed wetlands has been constructed on the premises of the Uttaranchal Jal Nigam Sewage treatment plant and municipal wastewater has been allowed to pass through the constructed wetland. It has been observed that BOD removal at 24 0C is governed by the equation Ce/Co = e-1.0042 x t and the removal rate constant of BOD has been observed to be 0.795 d-1 at 20 0C. It has been observed that the pilot-constructed wetland is bringing effluent BOD and TSS up to 20 mg/l and 30 mg/l respectively consistently for the duration under study. The values of hydraulic retention time, hydraulic loading, and organic loading for achieving this performance are found to be 1.75 days,13.22 cm/d, and 142.78 kg/ha. d respectively. Performance study of constructed wetland and the existing activated sludge treatment has shown that the percent removal for the parameters TSS, BOD, COD, and Fecal Coliforms is near to the same order except for TN and TP, where the activated sludge process unit shows higher removal than the constructed wetland.
Transition Relation Aware Self-Attention for Session-based Recommendation
Authors: Zhu Guanghui , Hou Haojun , Chen Jingfan , Yuan Chunfeng , Huang Yihua 
Published: November 2022

Abstract
Session-based recommendation is a challenging problem in real-world scenes, e.g., e-commerce, short video platforms, and music platforms,which aims to predict the next click action based on the anonymous session. Recently, graph neural networks (GNNs) have emerged as the state-of-the-art methods for session-based recommendation. However, we find that there exist some limitations in these methods. One is the important transition relations between items are not fully exploited. Another is the graph structure noise and long-range dependency problems when introducing GNNs in session-based recommendation. Considering these problems, we propose a novel approach based on self-attention mechanism for session-based recommendation, called Transition Relation Aware Self-Attention (TRASA). TRASA encodes the shortest path between items in a session graph through the gated recurrent unit as their transition relations. And uses a self-attention mechanism to explore the potential relations between items, which does not rely on the graph structure and can capture the long-range dependencies intrinsically. Besides, the transition relations are incorporated explicitly when computing the attention scores to keep the transition semantics. Extensive experiments on four real-word datasets demonstrate that TRASA outperforms the existing state-of-the-art methods consistently.
Language gene polymorphism pattern of Pan paniscus adds indirect evidence that Qinghai-Tibet plateau area was also a birth place of humankind: a potential milestone for both science and education
Authors: Xia Wei , Zhang Zhizhou 
Published: November 2022

Abstract
More and more evidences support that anthropoids in Africa originated from Asia, and why Homo genus emerged earlier in Africa than other continents remains enigmatic. One of the anthropoids closest to Homo is Pan paniscus (Bonobo), now inhabitating only south of Congo river. Language ability of Bonobo seems close to that of Homo sapiens, suggesting that it is evolutionarily only one or several steps away from humankind. In this study, 148 single nucleotide polymorphism (SNP) sites of 13 language genes were employed to roughly search for language gene polymorphism pattern (LGPP) of 113 whole genome sequences (including 59 ancient genomes ranging from 2000 BP to 120000 BP) from five continents (Africa, Asia, Europe, North America, and South America). It was found that the LGPP of Bonobo is highly close to several samples: a 7900y old ancient sub-Sahara African forager and three modern human people from Pakistan, Kenya and Congo. This result could be an indirect evidence for the following possibility: uplifting of Qinghai-Tibet plateau induced huge ecological challenge, thus promoting migration of ancient anthropoids around Pakistan area to Africa and evolution into Bonobo and a certain Homo genus that was highly similar to Bonobo. Later on the Homo genus migrated out of Africa and got a chance to reach Pakistan. This preliminary finding may have a potential to be a milestone in human evolution for both scientific research and cultural education.
Prevalence of multidrug-resistant Escherichia coli in household drinking water in rural Ghana
Authors: Kichana Elvis , Opare-Boafoa Maame Serwa , Bekoe Emmanuel Martin Obeng 
Published: November 2022

Abstract
Even in the 21st century, households in rural Ghana still rely on drinking water sources that are contaminated with pathogenic Escherichia coli, the consumption of which results in morbidity and mortality of children and adults. The present study sought to determine the prevalence and antimicrobial susceptibility profile of E. coli isolated from household drinking water. A total of 49 water samples were analyzed. E. coli was isolated and confirmed from the water samples using the spread plate and biochemical tests, respectively. The Kirby–Bauer method was used for antimicrobial susceptibility testing. E. coli was isolated from 79.6% of the water samples with a mean colony growth of 15 cfu/100 μl. The isolates were absolutely resistant to ceftazidime, cefixime, augmentin, and cefuroxime. In contrast, the isolates were most susceptible to ciprofloxacin, ofloxacin, gentamicin, and nitrofurantoin. Multidrug resistance was registered in 48.7% of the isolates. E. coli isolates from each water sample had a Multiple Antibiotic Resistance (MAR) index greater than 0.2, indicating increased use or misuse of antibiotics in the study area. This study revealed a high occurrence of multidrug-resistant E. coli and a record-high reduction in the efficacy of important antimicrobials.
Access to handwashing with soap facility: a post-sensitisation investigation of drivers
Authors: Limantol Andrew Manoba , Amoah Anthony 
Published: November 2022

Abstract
As part of measures to promote good hygiene, the United Nations International Children's Emergency Fund adopted affordable handwashing with soap facility (Tippy-Tap), and implemented sensitisation training for residents in the Binduri District of Ghana. In the context of good hygiene technology adoption, this study examines how poor communities have adopted and used Tippy-Tap over a period of 1 year. The study further identifies the associated post-sensitisation drivers of the Tippy-Tap. In addition to descriptive analysis, this study relies on post-sensitisation cross-sectional data and a logit regression with its marginal effects for the analysis. The results show that after the sensitisation programme, the adoption and use rate now stand at 90%, up from the baseline of 4%. The study provides evidence that female household-heads are more likely to adopt and use the Tippy-Tap relative to male household-heads. The study further reveals that being educated increases the probability of accessing the Tippy-Tap facility by about 23%. Subsequently, this study finds that personal, household, and community-level characteristics are the key drivers of the high adoption and use rate. In conclusion, the study finds evidence of a high adoption and use rate of the Tippy-Tap initiative after its sensitisation. The study recommends extensive sensitisation of hand hygiene using community-level social norms and practices.
Ubiquitous Existence of Cation-Proton Antiporter and its Structurefunction
Interplay: A Clinical Prospect
Authors: Dwivedi Manish , Mahendiran Sowdhamini 
Published: November 2022

Abstract:
Sodium, potassium, and protons are the most important ions for life on earth, and their homeostasis is crucially needed for the survival of cells. The biological cells have developed a system that regulates and maintains the integrity of the cells by facilitating the exchange of these ions. These systems include the specific type of ion transporter membrane proteins such as cation-proton antiporters. Cation proton antiporters induce the active transport of cations like Na+, K+ or Ca+ across the cell membrane in exchange for protons (H+) and make the organism able to survive in alkaline conditions, high or fluctuating pH, stressed temperature or osmolarity. The secondary transporter proteins exploit the properties of various specific structural components to carry out efficient active transport. Ec-NhaA crystal structure was resolved at acidic pH at which the protein is downregulated, which discloses the presence of 12 transmembrane (TM) helices. This structural fold, the “NhaA fold,” is speculated to contribute to the cation-binding site and conformational alterations during transport in various antiporters. Irrespective of the variation in the composition of amino acids and lengths of proteins, several other members of the CPA family, such as NmABST, PaNhaP, and MjNhaP1, share the common structural features of the Ec-NhaA. The present review elucidates the existence of CPAs throughout all the kingdoms and the structural intercorrelation with their function. The interplay in the structure-function of membrane transporter protein may be implemented to explore the plethora of biological events such as conformation, folding, ion binding and translocation etc.
Association Between ABCA1 R219K Variant and Alzheimer's Disease: An
Updated Meta-Analysis and Systematic Review
Authors: Zhao Jinrong , Wang Jinpei , Zhao Dong , Wang Lin , Luo Xiaoe 
Published: November 2022

Background:
Over a dozen studies have investigated the effect of the R219K variant in the
ATP-binding cassette transporter A1 (ABCA1) gene on the risk of Alzheimer's disease (AD), but the
results are conflicting.

Objective:
We performed a systematic review and meta-analysis to comprehensively assess the association
between the ABCA1 R219K variant and the risk of AD.

Methods:
Studies included in the meta-analysis were obtained by searching PubMed, Web of Science
and AlzGene. Review Manager 5.4 was used for meta-analysis. Both the odds ratio (OR) and its 95%
confidence interval (CI) were used to evaluate the effect of ABCA1 R219K polymorphism on AD susceptibility.
Heterogeneity between the included studies was assessed using I2 statistics and Cochran Qtest.
Funnel plots were used to assess publication bias.

Results:
A total of 14 eligible studies involving 10084 subjects were retrieved from PubMed, Web of
Science and AlzGene. Meta-analysis results showed that R219K polymorphism was significantly associated
with a decreased risk of AD in Chinese under a recessive model (OR = 0.67; 95% CI = 0.51-
0.88; P = 0.004).

Conclusion:
The present meta-analysis indicated that the KK genotype of R219K polymorphism may
act as a protective factor for AD in the Chinese population. Additional studies with larger sample sizes
are needed to further confirm this association.
Effect of Maxillary Expansion Treatment Protocols On Maxillary Sinus Volume, Pharyngeal Airway Volume, and Hyoid Bone Position: A Prospective, Cone Beam Computed Tomography (CBCT) Study
Authors: Shetty Nirali , Nambiar Supriya , Desai Asavari , Ahmed Junaid 
Published: November 2022

Aim:This study aimed to investigate the changes in maxillary sinus volume, pharyngeal airway volume, and hyoid bone position in patients treated with Rapid Maxillary Expansion (RME) and Alternate Rapid Maxillary Expansion and Constriction (AltRaMEC) – Facemask therapy and compare between the groups.Materials and Methods:The records of 15 patients between the age group of 8-14 years with Class I and Class III malocclusion were collected, and the patients were assigned into two groups. The first group comprised of 8patients (4 girls and 4 boys) who were treated with Rapid Maxillary Expansion. The second group comprised 7 patients (5 boys and 2 girls) treated with expansion devices using Alt-RaMEC protocol followed by maxillary protraction using a facemask. Pre and post-treatment CBCT scans were obtained and analyzed for changes in the pharyngeal airway, maxillary sinus volume, and hyoid bone position. The maxillary sinus volume was calculated in 3 dimensions by measuring the craniocaudal height, anteroposterior depth, and mediolateral width. Measurements were taken before and after the procedure to compare the groups and between the groups. The data were analyzed using Paired t-test and an Independent t-test.Results:In the RME group, a significant increase in the total maxillary sinus volume (mean difference = 520±576.57mm3), especially in the craniocaudal height, was seen, but no changes were noted in the pharyngeal airway and hyoid position. The AltRAMEC group showed a statistically significant increase in the maxillary sinus volume (anteroposteriorly) and nasopharyngeal volume with a p-value <0.005. Comparing the maxillary sinus volume changes in RME and AltRAMEC groups showed a statistically significant increase in the volume of the maxillary sinuses in both groups, significantly higher in the RME group.Conclusion:RME and AltRAMEC protocol followed by facemask therapy caused a significant increase in the maxillary sinus volume, while no changes were noted in the nasopharynx, oropharynx, hypopharynx, and hyoid bone position. While comparing the treatment effects between the groups, RME showed a greater increase in maxillary sinus volume (p < 0.05), specifically in the anteroposterior dimension. Since there are no changes in airway volume and hyoid position with the skeletal expansion protocols, there will be no difference in nasal resistance to airflow.