bluuebunny/crossref_metadata_2025_split huggingface dataset:
Did Angiodysplasia Associated with Heyde's Syndrome Disappear Spontaneously?: A case report
Authors: Li Rui , Zhang Jiechun , Ji Shuliang , Shi Jiaxi , Qing Lijin , Wu Wei 
Published: March 2022

Abstract
Background: Heyde’s syndrome can be easily overlooked or misjudged in clinical practice because it shares common clinical manifestations with multiple diseases and because of the limited accuracy of the corresponding examinations for diagnosing Heyde’s triad. Moreover, aortic valve replacement is often delayed in these patients because of the contradiction between anticoagulation and haemostasis. Herein, we present a rare case of atypical Heyde's syndrome. The patient's severe intermittent gastrointestinal bleeding was not completely cured even after a local enterectomy. In the absence of direct evidence of acquired von Willebrand syndrome (AVWS) or angiodysplasia, her long-standing gastrointestinal bleeding was finally stopped after receiving transcatheter aortic valve implantation (TAVI).Case presentation: A 64-year-old female suffered from refractory gastrointestinal bleeding and exertional dyspnoea. Local enterectomy was performed owing to persistent haemorrhage and repeated transfusions; subsequently, histological examination revealed angiodysplasia. Heyde's syndrome was not suspected until 3 years later, at which time the patient started bleeding again and was also found to have severe aortic valve stenosis on echocardiography. TAVI was consequently performed when the patient was in a stable condition even there was no evidence of angiodysplasia and AVWS at that time. The post-procedure and follow-up were uneventful.Conclusions: The visible characteristics of angiodysplasia or a shortage of HMWM-vWFs should not be indispensable for the clinical diagnosis of Heyde's syndrome. Enterectomy could be a bridging therapy for aortic valve replacement in patients with severe haemorrhage, and TAVI may benefit high-risk patients who are stable.
Correlation Between the TERT rs2736100 A/C Polymorphism and Susceptibility to Interstitial Lung Diseases: An Ethnicity-Based Meta-Analysis Study
Authors: Cui Beibei , Chen Yuehong , Ma Xinyue , Yin Geng , Xie Qibing 
Published: March 2022

Abstract
Objective: This meta-analysis aimed to investigate the correlation between the TERT rs2736100 A/C polymorphism and susceptibility to interstitial lung diseases (ILD).Methods: The systematic review was registered in PROSPERO. OVID MEDLINE, OVID EMBASE, and Web of Science electronic databases were searched. The meta-analysis effect size was estimated using either incidence with 95% confidence intervals (CIs) or odds ratio (OR). A funnel plot was used to assess the risk of publication bias.Results：A total of 12 eligible studies were finally analyzed, involving 5272 cases and 14767 controls. The frequency of rs2736100_C was lower in patients with ILD (38.4% vs 47.3%). Asian population had a lower rs2736100_C frequency than European population (32.8% vs 40%). A significant association between rs2736100_A and ILD was identified (p<0.00001, OR 1.36, 95% CI 1.24-1.48). The incidence of ILD increased with rs2736100_A in both European population (p<0.00001, OR 1.34, 95% CI 1.27-1.41) and Asian population (p=0.008, OR 1.42, 95% CI 1.10-1.85). Among six idiopathic pulmonary fibrosis (IPF) studies, rs2736100_A was associated with the incidence of IPF (p<0.00001, OR 1.53, 95% CI 1.27-1.85). All genetic models, including recessive, dominant, and additive models, showed increased risk of IPF was associated with rs2736100_A. Rs2736100_A was not associated with the incidence ofconnective tissue disease associated ILD (CTD-ILD) (p=0.75, OR 1.07, 95% CI 0.71-1.61).Conclusion: This study demonstrated the frequency of rs2736100_A was lower in Asian population. We confirmed the TERT rs2736100 A/C polymorphism was a strong risk factor for IPF, but not for CTD-ILD. Whether the incidence of CTD-ILD increases with the TERT rs2736100 A/C polymorphism needs to be evaluated in the future.
Determination of Level of Nicotine in Some Commercial cigarettes Available in Ethiopia using UV-Vis spectrometer
Authors: Yesgat Derejaw Woldemariam 
Published: March 2022

Abstract
Nicotine is a psychoactive chemical found in in cigarette. Level of nicotine in different cigarette brand are depends on different factor. Nicotine reacts with alkaline potassium permanganate to form a product having maximum absorbance (λmax) at 610 nm. This observation made use of in a UV-vis spectroscopic determination of level of nicotine content in some commercial cigarettes. The content of variety of cigarettes brands studied were found to be as follows: Marlboro = 16.4 ± 1.25 mg/g, Nyala premium = 12.27 ± 1.46mg/g, Rothmans = 11.27 ± 1.35mg/g, Gisila = 11.0 ± 1.56 mg/g, Ghamdane = 9.67 ± 1.30 mg/g, Nyalla = 8.93 ± 0.35 mg/g, Royal = 8.89 ± 0.25mg/g, Delight = 8.47 ± 0.45 mg/g, finally Kent cigarette were found to have lowest nicotine level which is 6.73 ± 0.87mg/g. the result of this research also confirmed that the nicotine content of Ethiopian cigarette brands (13.19 mg/cigarettes) was a little bit smaller than level of nicotine in imported brands (15.30mg/cigarette). However, level of nicotine in Ethiopian cigarette brands (10.17 ± 16mg/g) have almost equivalent nicotine level when expressed in milligram per gram with imported cigarette brands (10. 59 ± 3.49mg/g).
FCHO1 Is a Prognostic Biomarker of Colon Adenocarcinoma and Associated with Immune Infiltration and Glycolysis
Authors: Cheng Fei , Yuan Lebin , Wu Zhao , li Xiaodong , Shen Wei 
Published: March 2022

Abstract
Background
Colon adenocarcinoma (COAD) is a common digestive tract tumor and the molecular mechanism is very complicated. Overexpression of FCHO1 plays the role of oncogene or tumor suppressor gene in the process of tumorigenesis and development. However, possible mechanisms of FCHO1 in COAD remains unknown.
Methods
Online database Oncomine, TIMER and TCGA were used to clarify the expression level of FCHO1 in COAD. Using receiver operating characteristic (ROC) curve and GEIPA database to evaluate the prognostic value of FCHO1 in COAD. Then, STRING and GeneMANIA database were used to construct the protein-protein interaction network. The GO/KEGG enrichment analysis were performed by Using Funrich. Co-expression genes of FCHO1 were acquired by Linkedomics database, GSEA analysis was used to explore the possible pathway in co-expression genes of FCHO1. The correlation between FCHO1 expression and hypoxia-related genes, glycolysis-related genes and immune infiltrates was analyzed using the TIMER, Starbase and TCGA cohort.
Results
Our results revealed that high expression level of FCHO1 was significantly increased in COAD tissues than normal tissue. High expression of FCHO1 in COAD predicted worse survival, including OS (HR = 1.8 p = 0.0022), DFS (HR = 1.6 p = 0.043). GSEA analysis showed that co-expression genes were significantly linked with MicroRNAs in cancer, Oxidative phosphorylation, Cell cycle, Notch signaling pathway, VEGF signaling pathway and p53 signaling pathway. Further, the result revealed that hypoxia-related genes (NFKB1, VEGFB) was simultaneously positively correlated with FCHO1 expression in TIMER, Starbase database. Glycolysis-related genes (HK1, G6PD and SLC2A1) was positively associated with FCHO1 expression in TIMER, Starbase database. At the same time, PDCD1 and LAG3 expression, which as immune checkpoint, were positively correlated with FCHO1 expression.
Conclusion
Collectively, FCHO1 may act as a promising diagnostic and prognostic biomarker and correlated with hypoxia, glycolysis and immune infiltration in COAD.
Removal of Contaminant in Electroplating Waste Water and Its Toxic Effect Using Biosynthesized Silver Nanoparticles
Authors: Shittu Oluwatosin , Gara Theresa Yebo , Onyinye Ihebunna 
Published: March 2022

Abstract
The emergence of industries in developing countries is a rising challenge where the generation of undesirable liquid wastes containing pollutants are discharged into water bodies without adequate treatment. This research investigated the removal of contaminant in electroplating wastewater and its toxic effect using biosynthesized silver nanoparticles. Fresh leaves of Piliostigma thonningii were used to synthesize silver nanoparticles (Ag NPs). Dynamic light scattering (DLS), transmission electron microscope (TEM) and fourier transform infrared (FTIR) were used to characterize the produced Ag NPs. The electroplating wastewater was treated using green synthesized silver nanoparticle and subjected to physicochemical analysis using standard methods. The sub-chronic toxicity studies on the blood and liver tissues were carried out. At various conditions of concentration, temperature, pH and time, TEM showed the biosynthesized SNPs (1, 2, 3, and 4) to exhibit different particle size at 50 nm spherical shapes. FTIR revealed the presence of functional groups; -OH, -C = C, -C = O, -C-H and -NO. At different conditions, the SNPs demonstrated at the ability to remove pollutants in the electroplating wastewater with SNP4 displaying a higher affinity. In the toxicity evaluation of SNPs treated wastewater in rats, the heamatological parameters showed no significant influence on some parameters compared to the control group. However, the histopathological examination revealed toxic effects on the liver sections. It can be concluded that the biosynthesized Ag NPs contained functional groups that acts as stabilizing and reducing agent during the synthesis of Ag NPs had the ability to remove contaminant from electroplating wastewater.
Subjective well-being among medical staff: capturing mental health outcomes using the Warwick-Edinburgh Mental Well-Being Scale
Authors: Zhu Jianing , Dong Aishu , Huang Jing , Guo Wenjian 
Published: March 2022

Abstract
Background: There is growing awareness of the importance of subjective well-being (SWB) in the general population and specific groups. The mental health of medical staff influences the quality of their treatment. We performed this study to explore the SWB of medical staff using the Warwick-Edinburgh Mental Well-Being Scale (WEMWBS).Methods: In this cross-sectional study, we surveyed medical staff at several hospitals in China. We recorded demographics and the WEMWBS from a cross-sectional online multicenter survey from May 15 to July 15, 2020. Univariate analysis and structural equation modeling were used to identify factors correlated with the SWB among medical staff.Results: A total of 572 respondents completed the questionnaires. The mean age was 34.18 ± 6.36 years (21 to 55 years). The mean WEMWBS was 38.47 ± 13.23 (standard deviation = 13.23; 95% confidence interval 37.45 to 39.61). The factors influencing SWB were age, marital status, religious status, job type, daily working hours, number of night shifts per week, daily fruit and vegetable consumption, weekly exercise duration, self-reported personality type, general health status, and health status compared with one year before (P＜0.05).Conclusions: The medical staff's SWB level was lower than occupational groups. These data provide a baseline for future research in this area, especially for promoting SWB. More attention should be paid to improving SWB among medical staff.
Comments on "Informational laws of genome structures"
Authors: Andrade-Restrepo Martín , Alvarez Carlos É. 
Published: March 2022

Abstract
In this paper, we expose possible flaws in some of the proofs of the mathematical results presented by Bonnici and Manca, 2016 and suggest some corrected proofs of two of their results. We show that the possibility of overlap between k-mers given a fixed value of k (minimum length such that all k-mers are hapaxes) was not taken into account in several steps of the proof in Lemma 2, which makes the theorem false. For this we show a counterexample to the Lemma. In addition, we correct the proofs of Lemma 1 and Proposition 3. In the former case, we explain why the argument presented is incomplete and provide a correct proof. In the case of the latter, we show with a counter example that their claim is incorrect without explicitly mentioning the correct ranges of k over which it holds. We also provide a more general proof which accounts for this correction. We remark that although some of these comments invalidate the mathematical basis of their results, there is a possibility that the associated error becomes small in the limit n>>k, hence allowing for the apparent agreement with the data and the simulations.
Based on variability parameters analysis of autonomic function in rats with Parkinson's disease induced by rotenone
Authors: Yu Yi , Gao Xudong , Wang Mengke , Lei Miaoqing , Liu Yilin , Chen Peiqi , Zhao Xuewei , Feng Yifan , Hu Heshun , Niu Yanxiang , Wang Chong , Shao Zhenpeng , Wu Yang , Zhu Yu , Mu Junlin , Zhao Zongya 
Published: March 2022

Abstract
Background: Parkinson's disease is a common neurodegenerative disease, and while early detection of cardiovascular symptoms may aid in early diagnosis, there are no definitive markers to assess cardiovascular autonomic damage in Parkinson's disease. Methods: Twenty Sprague Dawley rats were randomly assigned to the experimental (n=12) and control (n=8) groups, and a model of Parkinson's disease was created in the experimental group by stereotaxic injection of rotenone into the substantia nigra compacta and ventral tegmental area. Telemetry implantation was carried out after successful modeling. Equal dosages of saline were injected into control animals at the same places. In both groups of rats, ECG, blood pressure, core temperature, and activity were recorded using a Data Science International implantable physiological signal telemetry device. The time domain, frequency domain, and non-linear analysis were used to examine the blood pressure and heart rate variability of the two groups of rats. Results: The experimental group had higher Detrended Fluctuation Analysis (DFA) of blood pressure signals (systolic, diastolic, and mean arterial pressure), mean arterial pressure, normalized high frequency power (nHF) than the control rats (p<0.05). The experimental group had lower sample entropy (SampEn) of blood pressure signals (diastolic and mean arterial pressure), root mean squared successive differences (RMSSD), normalized lower frequency power (nLF) and total power than the control group (p<0.05). However, the standard deviation, coefficient of variation, and continuous variation in the linear analysis of continuous blood pressure signal between the two groups were not statistically significant (p>0.05). Conclusion: The rats in the rotenone model had significant autonomic dysfunction, and non-linear analysis approaches such as detrended fluctuation analysis and sample entropy were able to discern the diseased condition of the rats more sensitively while processing the continuous blood pressure data.
Exacerbation Frequency, COPD Symptoms, and Health-Related Quality of Life of COPD Patients According to Post-Bronchodilator FEV1 – A Post-hoc Analysis of Pooled Data from Two Cross-Sectional Studies
Authors: Chai Chee-Shee , Ng Diana-Leh-Ching , Mos Sumastika Bt , Ibrahim Muhammad Amin B , Tan Seng-Beng , Pang Yong-Kek , Liam Chong-Kin 
Published: March 2022

Abstract
Background:This study aimed to compare and correlate the post-bronchodilator forced expiratory volume in one second (PB-FEV1), exacerbation frequency, and scores of modified Medical Research Council (mMRC), COPD Assessment Tool (CAT), and St George’s Respiratory Questionnaire for COPD (SGRQ-c) of COPD patients. Methods:This was a post-hoc analysis of pooled data from two cross-sectional studies conducted from 1st June 2017 to 31st May 2018 at the respiratory clinic of University of Malaya Medical Center, and from 23rd January 2018 to 22nd January 2019 at the outpatient clinics of the Serian Divisional Hospital and Bau District Hospital in Malaysia, respectively. The parameters measured included PB-FEV1, exacerbation frequency, mMRC, CAT, and SGRQ-c. Descriptive, association and correlation statistics were used. Results:Of a total of 374 patients, the PB-FEV1 predicted was < 30% in 85 (22.7%), 30 – 49% in 142 (38.0%), 50 – 79% in 111 (29.7%), and ≥ 80% in 36 (9.6%) patients. Patients with PB-FEV1 < 30% predicted had significantly more COPD exacerbations than those with PB-FEV1 30 – 49% predicted (p < 0.001), 50 – 79% predicted (p < 0.001), and ≥ 80% predicted (p = 0.002). The scores of mMRC, CAT and SGRQ-c were not significantly higher in patients with more severe airflow limitation based on PB-FEV1 (p = 0.121 – 0.271). The PB-FEV1 predicted had significant small negative correlations with exacerbation frequency (r = - 0.182, p < 0.001), mMRC (r = - 0.121, p = 0.020), and SGRQ-c scores (r = - 0.114, p = 0.028). There was a moderate positive correlation between COPD exacerbation frequency and scores of mMRC, CAT, and SGRQ-c (r = 0.407 – 0.482, all p < 0.001). There were significant strong positive correlations between mMRC score with CAT (r = 0.727) and SGRQ-c scores (r = 0.847), and CAT score with SGRQ-c score (r = 0.851) (all p < 0.001). Conclusions:In COPD patients, different severity of airflow limitation was not associated with significant differences in the dyspnea symptom and HRQOL. Exacerbation was significantly more frequent in patients with very severe airflow limitation only. The correlation between airflow limitation with exacerbation, dyspnea symptom, and HRQOL was weak.
Combined administration of anisodamine and neostigmine reduced Dextran Sulfate Sodium-Induced Colitis through inducing autophagy and inhibiting inflammation
Authors: Kong Ni , Ke Ping 
Published: March 2022

Abstract
Background: Our Previous work demonstrated that combined treatment of ANI (anisodamine) and NEO (neostigmine) produced anti-shock effect and rescued acute lethal crush syndrome via activating α7 nicotinic acetylcholine receptor (α7nAChR). In this study, we attemptted to reveal the therapeutic effect and underlying mechanisms of ANI/NEO combination in dextran sulfate sodium (DSS)-induced colitis.Methods: The expression of autophagy related proteins was detected by Western blot and the autophagy flux was evaluated by confocal microscopy transfected with tandem fluorescent AdPlus-mCherry-GFP-LC3B plasmid. The inflammatory cytokines were measured by RT-PCR and ELISA. Intestinal inflammation was reflected by HE staining.Results: Combining treatment of ANI and NEO at a ratio of 500:1 alleviated the symptoms of DSS-induced mice colitis, reduced body weight loss, improved the disease activity index, enhanced colon length and alleviated colon inflammation. Treatment with ANI/NEO combination also enhanced autophagy both in colon from DSS-induced colitis mice and Caco-2 cells stimulated with LPS/DSS. In addition, ANI/NEO significantly inhibited INF-γ, TNF-α, IL-6, and IL-22 in colon tissues and decreased TNF-α, IL-1β and IL-6 mRNA levels in Caco-2 cells. These effects were attenuated by autophagy inhibitor 3-methyladenine (3-MA) and ATG5 siRNA. Further more, autophagy inhibitor 3-MA and α7nAChR antagonist methyllycaconitine (MLA) weakened the protective effect of ANI/NEO on colitis in DSS-induced mice.Conclusion: Collectively, these results demonstrate that ANI/NEO combination could produce therapeutic effects in DSS-induced mice colitis and this effect is mediated by autophagy and a7nAChR.
Multimolecular Characteristics and Role of BRCA1 Interacting Protein C-Terminal Helicase 1 (BRIP1) in Human Tumors: A Pan-Cancer Analysis
Authors: Wang Ruohuang , Wang Lin , Cui Xin , Wang Shun , Chen Ting , Niu Yanfang , Du Xiaoyun , Kong Jingwen , Zhang Jisheng , Jiang Yan 
Published: March 2022

Abstract
Background
The aberrant expression of BRIP1 was associated with several cancers, the panoramic picture of BRIP1 in human tumors is unclear. The purpose of this study is to explore the panoramic picture of expression of BRIP1 which was associated with several cancers.
Methods
Based on the data from TCGA, we utilized online databases to systematically analyze the multimolecular characteristics of BRIP1 in 33 human tumors.
Results
We observed prognosis-related differential BRIP1 expression between various carcinomas and the corresponding normal tissues. “Basal transcription factors”, “Homologous recombination”, “Nucleotide excision repair”, and DNA metabolism pathways may play a role in the functional mechanisms of BRIP1. Patients with uterine corpus endometrial carcinoma presented with the highest alteration frequency of BRIP1 (near 10%). Single nucleotide and copy number variation of BRIP1 were noticed in multiple cancer, and the expression of BRIP1 is significantly regulated by copy number variation in breast invasive carcinoma and lung squamous cell carcinoma. BRIP1 expression was negatively correlated to the methylation level in many human tumors, and the expression was associated with the activation of apoptosis, cell cycle, and DNA damage response, and inhibition of hormone ER and RNS/MARK signaling pathways. Moreover, a positive correlation was observed between BRIP1 expression and the immune infiltration level of cancer-associated fibroblasts and CD8 + T cells in lung adenocarcinoma.
Conclusion
Our pan-cancer analysis of BRIP1 provide valuable resource for understanding the characteristics of BRIP1 across human cancers.
Microbial contamination and antimicrobial susceptibility pattern of bacterial isolates from sorghum and millet derived local beverage (Obushera) consumed in Mbarara City, Uganda
Authors: Kiprotich Amos , Wangalwa Rapheal , Atwebembeire Jeninah , Kagoro-Rugunda Grace 
Published: March 2022

Abstract
The high consumption and unconventional manner in which locally derived sorghum and millet beverages (Obushera) are processed in Uganda prompted the need to establish microbial safety of the beverages. Microbial contamination and its variation with storage time and temperature, antibiotic-susceptibility of pathogenic bacterial isolates, and nutritional content of Obushera (n = 96) were determined. Handling and hygiene practices of processors and vendors of Obushera (n = 28) were also determined. Among the four categories of Obushera found in Mbarara city, Ekitiribita had the highest total viable counts (TVC) with a median of 4.62 log CFU/ml, followed by Obwenkiga and Obutiire with a median of 4.31 and 4.25 log CFU/ml, respectively. Microbial contaminants isolated included: Providencia spp.7(7.3%), Coagulase-positive Staphylococci 23(24%), Pseudomonas spp.3(3.1%), Enterobacter cloacae 12 (12.5%), Enterobacter aerogens 3 (3.1%), Proteus vulgaris 15(15.6%), Klebsiella spp. 13(13.5%), Streptococcus spp. 7(7.3%), and Escherichia coli 11 (11.5%). Saccharomyces cerevisiae (65.6%), Candida spp. (28.1%), Mucor spp. and Aspergillus niger accounted for 52.1% contamination of all samples. The above microbial contaminants persisted with storage except Providencia spp., Proteus vulgaris, Mucor spp. and Aspergillus niger. Microbial and nutritional analyses showed a positive significant correlation between microbial counts and amino acid, carbohydrates, reducing sugar concentration and pH in Obushera (p-value < 0.05). All isolated strains of bacteria were resistant to more than one family of antibiotics used. The review of hygiene and handling practices revealed that they did not conform to good manufacturing practices neither did they meet the East African Standards of < 2 log10 CFU/ml TVC. Overall, this study revealed that Obushera consumed in Mbarara City, Uganda, is a high-risk beverage due to the high rate of microbial contamination and antibiotic resistance.