bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D reconstructed brain images reveal the possibility of the ogg1 gene to suppress the irradiation-induced apoptosis in embryonic brain in medaka (Oryzias latipes)
Authors: Yasuda Takako , Li Duolin , Sha Erge , Kakimoto Fumitaka , Mitani Hiroshi , Yamamoto Hiroshi , Ishikawa-Fujiwara Tomoko , Todo Takeshi , Oda Shoji 
Published: February 2022

Abstract
The accumulation of oxidative DNA lesions in neurons is associated with neurodegenerative disorders and diseases. Ogg1 (8-oxoG DNA glycosylase-1) is a primary repair enzyme to excise 7,8-dihydro-8-oxoguanine (8-oxoG), the most frequent mutagenic base lesion produced by oxidative DNA damage. We have developed ogg1-deficient medaka by screening with a high resolution melting (HRM) assay in Targeting-Induced Local Lesions In Genomes (TILLING) library. In this study, we identified that ogg1-deficient embryos have smaller brains than wild-type during the period of embryogenesis and larvae under normal conditions. To reveal the function of ogg1 when brain injury occurs during embryogenesis, we examined the induction of apoptosis in brains after exposure to gamma-rays with 10 Gy (137Cs, 7.3 Gy/min.) at 24 h post-irradiation both in wild-type and ogg1-deficient embryos. By acridine orange (AO) assay, clustered apoptosis in irradiated ogg1-deficient embryonic brains were distributed in a similar manner to those of irradiated wild-type embryos. To evaluate possible differences of gamma-ray induced apoptosis in both types of embryonic brains, we constructed 3D images of the whole brain based on serial histological sections. This analysis identified that the clustered apoptotic volume was about 3 times higher in brain of irradiated ogg1-deficient embryos (n = 3) compared to wild-type embryos (n = 3) (P = 0.04), suggesting that irradiation-induced apoptosis in medaka embryonic brain can be suppressed in the presence of functional ogg1. Collectively, reconstruction of 3D images can be a powerful approach to reveal slight differences in apoptosis induction post-irradiation.
Consistency Between Thyrotropin Receptor Antibody (TRAb) and Thyroid-Stimulating Antibody (TSAb) Levels in Patients with Graves Disease
Authors: Huang Youyuan , Jin Bo , Huang Yucheng , Dong Aimei 
Published: February 2022

Abstract

Objective
To investigate the consistency between thyrotropin receptor antibody (TRAb) and thyroid-stimulating antibody (TSAb) levels in patients with Graves disease (GD).

Methods
We performed a cross-sectional observational study to recruit eligible patients with GD who visited the outpatient endocrinology clinic for the purpose of evaluating the consistency between their TRAb and TSAb levels. Our cohort included 28 men and 99 women.

Results
The median levels of TRAb and TSAb were 5.65 IU/L and 3.76 IU/L, respectively, in the enrolled patients with GD. The levels of TRAb (5.03 vs 8.42 IU/L; P = .008) and TSAb (2.69 vs 5.37 IU/L; P = .008) in patients with adequate thyroid regulation were all lower than those in patients with inadequate thyroid regulation.

Conclusions
Although TRAb is closely related to TSAb, we observed high heterogeneity of TRAb due to relatively low consistency between the levels of the 2 antibodies.
Direct visualization of pre-protamine 2 detects protamine assembly failures and predicts ICSI success
Authors: Rezaei-Gazik Maryam , Vargas Alexandra , Amiri-Yekta Amir , Vitte Anne-Laure , Akbari Arvand , Barral Sophie , Esmaeili Vahid , Chuffart Florent , Sadighi-Gilani Mohammad Ali , Couté Yohann , Eftekhari-Yazdi Poopak , Khochbin Saadi , Rousseaux Sophie , Totonchi Mehdi 
Published: February 2022

Abstract
Histone-to-protamine transition is an essential step in the generation of fully functional spermatozoa in various mammalian species. In human and mouse, one of the two protamine-encoding genes produces a precursor pre-protamine 2 (pre-PRM2) protein, which is then processed and assembled. Here, we design an original approach based on the generation of pre-PRM2-specific antibodies to visualize the unprocessed pre-PRM2 by microscopy, flow cytometry and immunoblotting. Using mouse models with characterized failures in histone-to-protamine replacement, we show that pre-PRM2 retention is tightly linked to impaired nucleosome disassembly. Additionally, in elongating/condensing spermatids, we observe that pre-PRM2 and transition protein are co-expressed spatiotemporally, and their physical interaction suggests that these proteins act simultaneously rather than successively during histone replacement. By using our anti-human pre-PRM2 antibody, we also measured pre-PRM2 retention rates in the spermatozoa from 49 men of a series of infertile couples undergoing ICSI, which shed new light on the debated relation between pre-PRM2 retention and sperm parameters. Finally, by monitoring 2-pronuclei embryo formation following ICSI, we evaluated the fertilization ability of the sperm in these 49 patients. Our results suggest that the extent of pre-PRM2 retention in sperm, rather than pre-PRM2 accumulation per se, is associated with fertilization failure. Hence, anti-pre-PRM2 antibodies are valuable tools that could be used in routine monitoring of sperm parameters in fertility clinics, as well as in experimental research programmes to better understand the obscure process of histone-to-protamine transition.
Calcific tendinitis of the shoulder induced by an mRNA vaccine for COVID-19: A case report
Authors: Nakajima Koji , Miyata Akira , Kato So , Oshima Yasushi , Tanaka Sakae 
Published: February 2022

ABSTRACT
Coronavirus disease 2019 (COVID-19) vaccines have been widely used and have been shown to be effective in combating the pandemic. However, various side effects have been reported following vaccination. For instance, a condition called ‘shoulder injury related to vaccine administration’ (SIRVA) is characterized by shoulder pain and limited range of motion after intramuscular injection of a vaccine into the deltoid muscle of the shoulder. Despite an increase in SIRVA cases, the exact incidence of the disease is unclear, and there are a few reports of SIRVA about the COVID-19 vaccine. Here, we report a rare case of an 83-year-old woman who was diagnosed with calcification in her left shoulder 1 year ago and developed calcific tendinitis after receiving an mRNA vaccine for COVID-19 (Pfizer-BioNTech). Radiographs showed calcification of the supraspinatus tendon, and magnetic resonance images showed continuous inflammatory findings from the subdeltoid bursa to the subacromial bursa. We treated the patient with celecoxib and acetaminophen, and she recovered after about 2 months. In order to prevent SIRVA, the presence of shoulder joint disease should be carefully asked during a pre-vaccination assessment. The puncture point should be chosen with the median point of the deltoid muscle or the anterior–posterior axillary line as landmarks because the more cephalad the puncture position, the greater the chance of causing SIRVA.
Tobacco Couponing: A Systematic Review of Exposures and Effects on Tobacco Initiation and Cessation
Authors: Liber Alex C , Sánchez-Romero Luz María , Cadham Christopher J , Yuan Zhe , Li Yameng , Oh Hayoung , Cook Steven , Warner Kenneth E , Henriksen Lisa , Mistry Ritesh , Meza Rafael , Fleischer Nancy L , Levy David T 
Published: February 2022

Abstract

Introduction
Tobacco couponing continues to be part of contemporary tobacco marketing in the United States. We performed a systematic review of the evidence of tobacco product coupon receipt and redemption to inform regulation.

Aims and Methods
We searched EMBASE OVID and Medline databases for observational (cross-sectional and longitudinal) studies that examined the prevalence of tobacco coupon receipt and coupon redemption across different subpopulations, as well as studies of the association between coupon receipt and redemption with tobacco initiation and cessation at follow-up. We extracted unadjusted and adjusted odds ratios for the associations between coupon exposure (receipt, redemption) and tobacco use outcomes (initiation, cessation) and assessed each studies’ potential risk of bias.

Results
Twenty-seven studies met the criteria for inclusion. Of 60 observations extracted, 37 measured coupon receipt, nine measured coupon redemption, eight assessed tobacco use initiation, and six assessed cessation. Tobacco product coupon receipt and redemption tended to be more prevalent among younger adults, women, lower education individuals, members of sexual and gender minorities, and more frequent tobacco users. Coupon receipt at baseline was associated with greater initiation. Coupon receipt and redemption at baseline were associated with lower cessation at follow-up among tobacco users. Results in high-quality studies did not generally differ from all studies.

Conclusions
Tobacco product coupon receipt and redemption are often more prevalent among price-sensitive subpopulations. Most concerning, our results suggest coupon receipt may be associated with higher tobacco initiation and lower tobacco cessation. Couponing thereby increases the toll of tobacco use and could prove to be a viable public health policy intervention point.

Implications
A systematic review was conducted of the scientific literature about the receipt, redemption, and effects on tobacco initiation and cessation of tobacco product couponing. This review found that tobacco coupons are more often received by price-sensitive persons and these coupons serve to increase tobacco initiation and decrease tobacco cessation. Policy efforts to address these consequences may help curb tobacco’s harms and address health inequities.
Predictors of Treatment Success After Periprosthetic Joint Infection: 24-Month Follow up From a Multicenter Prospective Observational Cohort Study of 653 Patients
Authors: Davis Joshua S , Metcalf Sarah , Clark Benjamin , Robinson J Owen , Huggan Paul , Luey Chris , McBride Stephen , Aboltins Craig , Nelson Renjy , Campbell David , Solomon L Bogdan , Schneider Kellie , Loewenthal Mark R , Yates Piers , Athan Eugene , Cooper Darcie , Rad Babak , Allworth Tony , Reid Alistair , Read Kerry , Leung Peter , Sud Archana , Nagendra Vana , Chean Roy , Lemoh Chris , Mutalima Nora , Tran Ton , Grimwade Kate , Sehu Marjoree , Looke David , Torda Adrienne , Aung Thi , Graves Steven , Paterson David L , Manning Laurens 
Published: February 2022

Abstract

Background
Periprosthetic joint infection (PJI) is a devastating condition and there is a lack of evidence to guide its management. We hypothesized that treatment success is independently associated with modifiable variables in surgical and antibiotic management.

Methods
The is a prospective, observational study at 27 hospitals across Australia and New Zealand. Newly diagnosed large joint PJIs were eligible. Data were collected at baseline and at 3, 12, and 24 months. The main outcome measures at 24 months were clinical cure (defined as all of the following: alive, absence of clinical or microbiological evidence of infection, and not requiring ongoing antibiotic therapy) and treatment success (clinical cure plus index prosthesis still in place).

Results
Twenty-four-month outcome data were available for 653 patients. Overall, 449 patients (69%) experienced clinical cure and 350 (54%) had treatment success. The most common treatment strategy was debridement and implant retention (DAIR), with success rates highest in early postimplant infections (119 of 160, 74%) and lower in late acute (132 of 267, 49%) and chronic (63 of 142, 44%) infections. Selected comorbidities, knee joint, and Staphylococcus aureus infections were independently associated with treatment failure, but antibiotic choice and duration (including rifampicin use) and extent of debridement were not.

Conclusions
Treatment success in PJI is associated with (1) selecting the appropriate treatment strategy and (2) nonmodifiable patient and infection factors. Interdisciplinary decision making that matches an individual patient to an appropriate management strategy is a critical step for PJI management. Randomized controlled trials are needed to determine the role of rifampicin in patients managed with DAIR and the optimal surgical strategy for late-acute PJI.
Controlling trapping, overgrazing and invasive vegetation is key to saving Java’s last population of the Black-winged Myna
Authors: Squires Thomas M , Collar Nigel J , Devenish Christian , Owen Andrew , Pratiwi Arif , Winarni Nurul L , Marsden Stuart J 
Published: February 2022

AbstractThe Black-winged Myna (Acridotheres melanopterus) is an Endangered passerine endemic to the islands of Java and Bali, Indonesia. Illegal trapping to supply the cage-bird trade has led to its near-total extinction, with the global population estimated to number fewer than 100 individuals. We estimated the current range and population size of the species at Baluran National Park, which supports Java’s last known population, and used species distribution modeling to evaluate potential suitability of currently unoccupied areas across the park to identify priorities for management intervention. We estimate that the Black-winged Myna population numbers 179 individuals (95% CI: 111–288; density: 14.3 ± 3.5 individuals km–2) and that its current range is 12.3 km2. Our model indicated that some 72 km2 of the park (30% of total area) has potentially suitable habitat for the species, and we infer that the principal cause for the disparity between its current and potential range is trapping, compounded by savanna loss and degradation due to illegal domestic cattle grazing and the spread of invasive thorny acacia (Vachellia nilotica). The partial clearance of acacia in recent years appears to have assisted a modest population recovery by the myna. Its further population growth and range expansion in Baluran will depend on effective management of illegal poaching, further clearance of acacia, and easing domestic cattle grazing pressure on areas of savanna, particularly through engagement with communities living inside the park. Any actions that increase the size of the Black-winged Myna population are likely to benefit other threatened savanna-dependent wildlife in the park, notably banteng (Bos javanicus) and Green Peafowl (Pavo muticus). While our models and recommendations may be applicable to other protected areas in Java, and indeed other threatened myna species, trapping and habitat change may have site-specific dimensions, especially outside of protected areas, and thus demand local bespoke solutions.
Photosystem I light-harvesting proteins regulate photosynthetic electron transfer and hydrogen production
Authors: Ho Thi Thu Hoai , Schwier Chris , Elman Tamar , Fleuter Vera , Zinzius Karen , Scholz Martin , Yacoby Iftach , Buchert Felix , Hippler Michael 
Published: February 2022

Abstract
Linear electron flow (LEF) and cyclic electron flow (CEF) compete for light-driven electrons transferred from the acceptor side of photosystem I (PSI). Under anoxic conditions, such highly reducing electrons also could be used for hydrogen (H2) production via electron transfer between ferredoxin and hydrogenase in the green alga Chlamydomonas reinhardtii. Partitioning between LEF and CEF is regulated through PROTON-GRADIENT REGULATION5 (PGR5). There is evidence that partitioning of electrons also could be mediated via PSI remodeling processes. This plasticity is linked to the dynamics of PSI-associated light-harvesting proteins (LHCAs) LHCA2 and LHCA9. These two unique light-harvesting proteins are distinct from all other LHCAs because they are loosely bound at the PSAL pole. Here, we investigated photosynthetic electron transfer and H2 production in single, double, and triple mutants deficient in PGR5, LHCA2, and LHCA9. Our data indicate that lhca2 and lhca9 mutants are efficient in photosynthetic electron transfer, that LHCA2 impacts the pgr5 phenotype, and that pgr5/lhca2 is a potent H2 photo-producer. In addition, pgr5/lhca2 and pgr5/lhca9 mutants displayed substantially different H2 photo-production kinetics. This indicates that the absence of LHCA2 or LHCA9 impacts H2 photo-production independently, despite both being attached at the PSAL pole, pointing to distinct regulatory capacities.
Order Flows and Financial Investor Impacts in Commodity Futures Markets
Authors: Ready Mark J , Ready Robert C 
Published: February 2022

Abstract
Using intraday data, we document statistically strong, but temporary, impacts of commodity index trade flows on commodity futures prices. We also examine the previously documented positive returns around the issuance of commodity-linked notes and find that these returns are an order of magnitude too large to be caused by the small trades necessary to hedge the notes. We provide new evidence that they are instead the result of endogenous issuance. Our results provide novel support for commodity financialization but highlight the importance of measuring the magnitude of financial investment, since even large financial flows have economically modest impacts on prices.
A multicentric validation study of a novel home sleep apnea test based on peripheral arterial tonometry
Authors: Van Pee Bart , Massie Frederik , Vits Steven , Dreesen Pauline , Klerkx Susie , Bijwadia Jagdeep , Verbraecken Johan , Bergmann Jeroen 
Published: February 2022

Abstract

Study Objectives
This paper reports on the multicentric validation of a novel FDA-cleared home sleep apnea test based on peripheral arterial tonometry (PAT HSAT).

Methods
One hundred sixty-seven participants suspected of having obstructive sleep apnea (OSA) were included in a multicentric cohort. All patients underwent simultaneous polysomnography (PSG) and PAT HSAT, and all PSG data were independently double scored using both the recommended 1A rule for hypopnea, requiring a 3% desaturation or arousal (3% Rule), and the acceptable 1B rule for hypopnea, requiring a 4% desaturation (4% Rule). The double-scoring of PSG enabled a comparison of the agreement between PAT HSAT and PSG to the inter-rater agreement of PSG. Clinical endpoint parameters were selected to evaluate the device’s ability to determine the OSA severity category. Finally, a correction for near-boundary apnea–hypopnea index values was proposed to adequately handle the inter-rater variability of the PSG benchmark.

Results
For both the 3% and the 4% Rules, most endpoint parameters showed a close agreement with PSG. The 4-way OSA severity categorization accuracy of PAT HSAT was strong, but nevertheless lower than the inter-rater agreement of PSG (70% vs 77% for the 3% Rule and 78% vs 81% for the 4% Rule).

Conclusions
This paper reported on a multitude of robust endpoint parameters, in particular OSA severity categorization accuracies, while also benchmarking clinical performances against double-scored PSG. This study demonstrated strong agreement of PAT HSAT with PSG. The results of this study also suggest that different brands of PAT HSAT may have distinct clinical performance characteristics.
Preventing postpartum insomnia by targeting maternal versus infant sleep: a protocol for a randomized controlled trial (the Study for Mother-Infant Sleep “SMILE”)
Authors: Quin Nina , Tikotzky Liat , Stafford Lesley , Fisher Jane , Bei Bei 
Published: February 2022

AbstractSymptoms of insomnia are common during the perinatal periods and are linked to adverse parent/infant outcomes. Theories on insomnia development (e.g. 3P model) suggest that significant sleep disruption (e.g. nighttime infant care) can precipitate, while unhelpful sleep-related cognitions/behaviors can perpetuate parental insomnia symptoms. This study aims to examine how two interventions, one addressing infant sleep as the precipitator, the other targeting maternal sleep-related cognitions/behaviors as the perpetuator, might prevent postpartum insomnia. Participants are 114 nulliparous females 26 to 32 weeks gestation, with self-reported insomnia symptoms (Insomnia Severity Index scores ≥ 8). Participants are randomized to one of three conditions and receive: (1) a “responsive bassinet” used until 6 months postpartum, designed to boost/consolidate infant sleep and target infant sleep as a precipitator of insomnia, (2) therapist-assisted cognitive behavioral therapy for insomnia, addressing unhelpful sleep-related cognitions/behaviors as perpetuators of insomnia, or (3) a sleep hygiene booklet (control condition). The primary outcome is maternal insomnia symptoms. Secondary outcomes include maternal sleep duration/quality, mental health (e.g. depression, anxiety), and wellbeing-related variables (e.g. sleep-related impairment). Outcomes are assessed using validated instruments at 26–32 and 35–36 weeks’ gestation, and 2, 6, and 12 months postpartum. This study adopts an early-intervention approach and longitudinally compares two distinct approaches to prevent postpartum insomnia in an at-risk population. If interventions are efficacious, findings will demonstrate how interventions targeting different mechanisms mitigate insomnia symptoms in perinatal populations. This will provide empirical evidence for future development of multi-component sleep intervention to improve mother-infant wellbeing.Clinical Trial Registration: The Study for Mother-Infant Sleep (The SMILE Project): reducing postpartum insomnia using an infant sleep intervention and a maternal sleep intervention in first-time mothers. https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=377927, Australian New Zealand Clinical Trials Registry: ACTRN12619001166167.
Space–time risk cluster and time trends of congenital syphilis in Brazil: an ecological study
Authors: Dalazen Chaiane Emília , de Souza Albert Schiaveto , Ribeiro Caique Jordan Nunes , Marques dos Santos Marquiony , Probst Livia Fernandes , Theobald Melina Raquel , De-Carli Alessandro Diogo 
Published: February 2022

Abstract

Background
Congenital syphilis (CS) is a problem of great concern for public health, especially in Brazil. The aim of this study was to analyse the time trends and the space–time dynamics of morbidity and mortality from CS in Brazil.

Methods
An ecological and time series study, which included all cases and deaths from CS recorded in a national Brazilian database from 2013 to 2019 was performed. Time trends in CS incidence and mortality were assessed using segmented linear regression. Univariate global and local Moran indices and space–time scan statistics were used in the space and space–time analyses.

Results
A total of 183 171 cases and 2401 deaths from CS were recorded in Brazil, with the highest number of cases being observed in the Southeast Region (n=82 612 [45.1%]). Only 21.1% of pregnant mothers with syphilis received adequate treatment. There was an upward trend in CS rates among mothers ages 20–29 y (average annual percent change [AAPC] 1.4 [95% confidence interval {CI} 1.0 to 1.7]) and with &lt;8 y of schooling (AAPC 6.6 [95% CI 5.3 to 7.9]). The primary space–time cluster involved 338 municipalities in the Southeast Region (relative risk 3.06, p&lt;0.001) and occurred between 2017 and 2019.

Conclusions
To reduce the trends in CS rates, it is necessary to develop actions to improve the quality of prenatal care and expand early diagnosis and adequate treatment of syphilis in pregnant women and their sexual partners, especially in groups with upward trends (mothers ages 20–29 y and &lt;8 y of schooling) and living in higher-risk regions (Southeast, North and Northeast).