bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between the Neutrophil-To-Lymphocyte Ratio and Diabetes Secondary to Exocrine Pancreatic Disorders
Authors: Chen Guanhua , Tan Chunlu , Liu Xubao , Chen Yonghua 
Published: July 2022

BackgroundDiabetes mellitus among patients with exocrine pancreatic disorders is commonly known to be associated with chronic inflammation, including chronic pancreatitis and pancreatic ductal adenocarcinoma (PDAC). The neutrophil-to-lymphocyte ratio (NLR) is a novel marker that indicates the presence of various chronic inflammatory diseases, including type 2 diabetes (T2DM). However, no studies have examined the relationship between the NLR value and diabetes secondary to exocrine pancreatic disorders.AimTo determine whether the NLR value is associated with diabetes secondary to exocrine pancreatic disorders.MethodsThe medical data of subjects with confirmed pancreatic disease who were admitted to the Department of Pancreatic Surgery of our institution from August 2017 to October 2021 were obtained from the database and retrospectively analyzed. Anthropometric measures, laboratory data, including HbA1c, fasting insulin, and fasting C-peptide levels and the inflammatory index (white blood cell count, NLR, platelet-to-lymphocyte ration, monocyte-to-lymphocyte ratio) were recorded. The NLR is the ratio of neutrophils to lymphocytes. A homeostasis model (HOMA-B and HOMA-IR) was used to measure beta-cell dysfunction and insulin resistance.ResultsThe NLR values of the diabetes secondary to exocrine pancreatic disorders group were significantly higher than those of the nondiabetic group (P=0.001). In multivariate logistic regression, after adjusting for covariates, high NLR values were found to be an independent risk factor for diabetes secondary to exocrine pancreatic disorders (OR: 1.37, 95% CI: 1.138-1.649, P=0.001). According to Spearman correlation analysis, the NLR was significantly correlated with fasting plasma glucose levels (P&lt;0.0001) and HOMA2-IR values (P=0.02).ConclusionThe NLR inflammation marker was significantly higher in subjects with diabetes secondary to exocrine pancreatic disorders and was associated with insulin resistance. NLR values may be reliable predictive markers for diabetes among patients with exocrine pancreatic disorders.
Relative viability proxy of airborne prokaryotic microorganisms at the Southeastern Mediterranean coastal Sea
Authors: Rahav E. , Paytan A. , Herut B. 
Published: July 2022

The atmosphere plays a fundamental role in transporting airborne prokaryotes across the oceans and land. Despite the harsh atmospheric conditions, a considerable fraction of the airborne prokaryotic microorganisms survive the journey and remain viable upon deposition, and can affect the receiving environment. Here, we provide the first estimate of potential viability proxy for airborne prokaryotic cells at the Southeastern Mediterranean coast in 22 events during 2015, representing marine and terrestrial air-mass trajectories and a significant dust storm event. This was assessed using sequence amplicons of the small subunit ribosomal RNA gene (SSU rRNA) jointly with other complementary measurements. To estimate the relative viability in our dataset we used the ratio between the abundance of the bacterial SSU rRNA transcripts in a given sampling date and the lowest measured value (23.7.2015) as a measure of a relative viability proxy. The abundance of prokaryotes SSU rRNA transcripts ranged from ∼500 to 11,000 copies m3, with ∼2-fold higher relative viability proxy in marine-origin aerosols than predominantly terrestrial atmospheric trajectories. The relative viability proxy of prokaryotes was low during the peak of an intense and prolonged dust storm, and increased by ∼1.5-fold in the subsequent days representing background conditions (&lt;1700 ng Al m−3). Furthermore, we show that anthropogenic/toxic trace-metals (Cu/Al, Pb/Al) negatively correlates with potentially viable airborne prokaryotes in marine trajectory aerosols, whereas mineral dust load (Al, Fe proxy) positively affect their potential viability proxy. This may suggest that airborne prokaryotes associated to marine trajectories benefit from a particle-associate lifestyle, enabling relatively higher humidity and supply of nutrients attributed to mineral dust particles.
Co- and Post-seismic Deformation Mechanisms of the 2020 Mw6.0 Jiashi Earthquake in Xinjiang (China), Revealed by Sentinel-1 InSAR Observations
Authors: Wang T. , Zhu S. N. , Yang C. S. , Wei Y. J. , Zhao C.Y. , Hou H.C. 
Published: July 2022

On 19 January 2020, an Mw6.0 earthquake occurred in Jiashi County, Xinjiang, China. This earthquake is a strong earthquake that occurred in the Kepingtage Belt. The monitoring and inversion of the co-seismic and post-earthquake will help further understand the geometry and movement properties of this tectonic belt. In this study, Sentinel-1A images were used to analyze the deformation of co-seismic and post-seismic events. The Okada elastic dislocation model was used to invert the geometric parameters of the fault and co-seismic slip distribution. The results showed that the maximum uplift and maximum subsidence deformations from the ascending images were 55 and 45 mm, respectively. The maximum uplift and subsidence deformations from the descending images were 62 and 28 mm, respectively. The inversion results show that the earthquake was induced by a fault with a length of 23.5 km, width of 4.7 km, and depth of 7.2 km. This earthquake was a typical dip-slip event. The distributed inversion results of post-earthquake deformation show that the maximum co-seismic slip and maximum post-seismic slip are located on the same fault plane, mainly distributed on the edge of the co-seismic fault, between the two faults.
Coprecipitation of Organic Matter, Phosphate With Iron: Implications for Internal Loadings of Phosphorus in Algae-Dominated and Macrophyte-Dominated Lakes
Authors: Dai JiaRu , Zhang QiaoYing , Liu JingJing , Wen ShuaiLong , Zhang YuFeng , He Ding , Du YingXun 
Published: July 2022

Coprecipitation with iron (Fe) plays an essential role in the biogeochemical cycles of organic carbon (OC) and phosphorus (P) in lakes. The sources and composition of organic matter (OM) mediate its association with iron, which could thus influence the immobilization of phosphorus. In this study, water-soluble organic matter from the sediments of two typical states of shallow lakes, macrophyte-dominated zones (M-WSOM) and algae-dominated zones (A-WSOM), was extracted, and the ternary coprecipitation of WSOM, phosphate with Fe(III), was investigated. The ternary coprecipitation process was enhanced with increasing Fe(III) or decreasing pH value. It was found that pH of 6.5 was more favorable for coprecipitation than a pH of 7.5 or 8.5. At pH 6.5, the complexation between WSOM and Fe(III) occurred at the low Fe(III) inputs, while the coprecipitation of phosphate, WSOM with Fe(III) took place when Fe(III) inputs reached 40 μM. The presence of A-WSOM showed stronger inhibition on the coprecipitation of phosphate than that by M-WSOM. The formed ternary coprecipitates with A-WSOM had lower C/Fe ratios (0.13–2.78) than those with M-WSOM (1.28–4.05), which was because A-WSOM had lower aromaticity than M-WSOM. In addition, more functional groups in A-WSOM could complex with Fe(III), resulting in less immobilization of OC and P during the coprecipitation of A-WSOM, phosphate, and Fe(III). Our results demonstrated that in algae-dominated zones, more phosphorus remained soluble during the ternary coprecipitation, which could perform positive feedback on the growth of phytoplankton and provide a novel explanation for the difficulty in restoring eutrophic lakes.
Insights Into miRNA-mRNA Regulatory Mechanisms of Cold Adaptation in Gymnocypris eckloni: Ubiquitin-Mediated Proteolysis Is Pivotal for Adaptive Energy Metabolism
Authors: Nie Miaomiao , Ni Weilin , Wang Lihan , Gao Qiang , Liu Dan , Tian Fei , Wang Zhenji , Zhang Cunfang , Qi Delin 
Published: July 2022

This study aimed to understand cold stress adaptations mechanism in fish. Thus, the transcriptional response to cold conditions in Gymnocypris eckloni was evaluated using RNA-seq and microRNA (miRNA)-seq analyses. Low-temperature (LT) group G. eckloni was cultivated outdoors in waters cooled to 2–4°C for 3 weeks, while individuals in the control temperature (CT) group were exposed to 14–16°C. Significantly different responses were observed in both mRNA and miRNA expression profiles, with more mRNAs (1,833 and 1,869 mRNAs were up- and downregulated, respectively) and fewer miRNAs (15 and 6 were up- and downregulated, respectively) observed in the LT group individuals relative to the CT group individuals. A miRNA-mRNA network involved in the regulation of G. eckloni responses to cold stress was constructed; this network included ubiquitin-mediated proteolysis, protein processing, and oxidative phosphorylation. These results provided new insights into mechanisms of cold tolerance by fish, including decreased metabolic activity in addition to proteolysis.
WDR36 Safeguards Self-Renewal and Pluripotency of Human Extended Pluripotent Stem Cells
Authors: An Shiyu , Yao Dan , Zhang Wenyi , Sun Hao , Yu Tianyi , Jia Ruizhe , Yang Yang 
Published: July 2022

Extended pluripotent stem cells (EPS cells) have unlimited self-renewal ability and the potential to differentiate into mesodermal, ectodermal, and endodermal cells. Notably, in addition to developing the embryonic (Em) lineages, it can also make an effective contribution to extraembryonic (ExEm) lineages both in vitro and in vivo. However, multiple mysteries still remain about the underlying molecular mechanism of EPS cells’ maintenance and developmental potential. WDR36 (WD Repeat Domain 36), a protein of 105 kDa with 14 WD40 repeats, which may fold into two β-propellers, participates in 18sRNA synthesis and P53 stress response. Though WDR36 safeguards mouse early embryonic development, that is, homozygous knockout of WDR36 can result in embryonic lethality, what role does WDR36 plays in self-renewal and differentiation developmental potential of human EPS cells is still a subject of concern. Here, our findings suggested that the expression of WDR36 was downregulated during human hEPS cells lost self-renewal. Through constructing inducible knockdown or overexpressing WDR36-human EPS cell lines, we found that WDR36 knockdown disrupted self-renewal but promoted the mesodermal differentiation of human EPS cells; however, overexpressing of WDR36 had little effect. Additionally, P53 inhibition could reverse the effects of WDR36 knockdown, on both self-renewal maintenance and differentiation potential of human EPS cells. These data implied that WDR36 safeguards self-renewal and pluripotency of human EPS cells, which would extend our understanding of the molecular mechanisms of human EPS cells’ self-renewal and differentiation.
Cuproptosis-Related lncRNAs are Biomarkers of Prognosis and Immune Microenvironment in Head and Neck Squamous Cell Carcinoma
Authors: Yang Liuqing , Yu Jinling , Tao Lu , Huang Handan , Gao Ying , Yao Jingjing , Liu Zhihui 
Published: July 2022

Background: Cuproptosis is a new type of cell death that induces protein toxic stress and eventually leads to cell death. Hence, regulating cuproptosis in tumor cells is a new therapeutic approach. However, studies on cuproptosis-related long noncoding RNA (lncRNA) in head and neck squamous cell carcinoma (HNSC) have not been found. This study aimed to explore the cuproptosis-related lncRNAs prognostic marker and their relationship to immune microenvironment in HNSC by using bioinformatics methods.Methods: RNA sequencing, genomic mutations, and clinical data of TCGA_HNSC were downloaded from The Cancer Genome Atlas. HNSC patients were randomly assigned to either a training group or a validation cohort. The least absolute shrinkage and selection operator Cox regression and multivariate Cox regression models were used to determine the prognostic model in the training cohort, and its independent prognostic effect was further confirmed in the validation and entire cohorts.Results: Based on previous literature, we collected 19 genes associated with cuproptosis. Afterward, 783 cuproptosis-related lncRNAs were obtained through coexpression. Cox model revealed and constructed eight cuproptosis-related lncRNAs prognostic marker (AL132800.1, AC090587.1, AC079160.1, AC011462.4, AL157888.1, GRHL3-AS1, SNHG16, and AC021148.2). Patients were divided into high- and low-risk groups based on the median risk score. The Kaplan–Meier survival curve revealed that the overall survival between the high- and low-risk groups was statistically significant. The receiver operating characteristic curve and principal component analysis demonstrated the accurate prognostic ability of the model. Univariate and multivariate Cox regression analysis showed that risk score was an independent prognostic factor. In addition, we used multivariate Cox regression to establish a nomogram of the predictive power of prognostic markers. The tumor mutation burden showed significant differences between the high- and low-risk groups. HNSC patients in the high-risk group responded better to immunotherapy than those in the low-risk group. We also found that risk scores were significantly associated with drug sensitivity in HNSC.Conclusion: In summary, our study identified eight cuprotosis-related lncRNAs signature of HNSC as the prognostic predictor, which may be promising biomarkers for predicting the benefit of HNSC immunotherapy as well as drug sensitivity.
Case Report: Pulmonary sarcomatoid carcinoma complicating TP53 mutation treated successfully with Tislelizumab combined with Anlotinib—a case report
Authors: Li Yu-Feng , Zhao Xin-Fei , Tian Yue , Xiao Xin-Yao , Yan Cai-Yun , Shen Hua 
Published: July 2022

Pulmonary sarcomatoid carcinoma (PSC) is a rare subtype of lung malignant tumor. Conventional chemotherapy has a suboptimal effectiveness. PSC has the characteristics of rapid disease progression and poor prognosis. We herein report a 56-year-old male patient with substantial smoking history was pathologically diagnosed as PSC, cT4N0M0 IIIA stage. Peripheral blood NGS showed TP53 mutation. The patient had poor tolerance to the first-line chemotherapy regimen “albumin paclitaxel + cisplatin,” but the severe anemia was significantly improved after 5 days of anti-angiogenic therapy with Anlotinib. At this time, the patient received anti-PD-1 immunotherapy with Tislelizumab. Half a month later, degree III liver injury occurred repeatedly. After excluding drug-induced liver injury, we found that HCV-RNA 3.10 × 105 IU/ml and suspended all anti-tumor therapy. After the start of anti-HCV treatment with Epclusa, the treatment of Tislelizumab combined with Anlotinib was restarted, and there was no liver injury after that. The patient received monthly maintenance therapy with Tislelizumab combined with Anlotinib to the present. The pulmonary lesions continued to decrease, and only one lung cavity is left. The patient has achieved clinical complete remission (CCR) with PSF over 20 months. Our findings suggest that Tislelizumab combined with Anlotinib may be a preferred strategy in PSC complicating TP53 mutation. Core tip: Immune-check point inhibitors (ICIs) have been reported for the treatment of PSC in a small number of case reports and retrospective analysis, but there are few reports of ICIs combined with anti-angiogenic drugs. This patient was diagnosed as locally advanced PSC complicated with TP53 mutation and hepatitis C. After 14 cycles of Tislelizumab combined with Anlotinib treatment (during the course of treatment, several courses were not treated on time for economic reasons, rather than adverse reactions), the patient has achieved CCR. III degree liver injury occurred during the treatment, and the liver function returned to normal range after anti-hepatitis C treatment, which did not affect the continued treatment of this regimen.
Changes in Th9 and Th17 lymphocytes and functional cytokines and their relationship with thyroid-stimulating hormone receptor antibodies at different stages of graves’ disease
Authors: Ren Xuan , Chen Hui 
Published: July 2022

ObjectiveGraves’ disease (GD) is an organ-specific autoimmune disease characterized by the production of thyroid-stimulating antibodies (TSAb). The newly discovered CD4+ T helper cells, Th9 and Th17 lymphocytes, have been confirmed to be closely associated with a variety of immune diseases. However, relationships with the onset and development of GD remain unclear. The purpose of this study was to investigate the roles of Th9 and Th17 in the pathogenesis and prognosis of GD.PatientsWe recruited 26 patients with newly diagnosed GD, 45 patients with GD in remission, and 20 healthy individuals.MeasurementsThyroid function and autoantibodies were evaluated using chemiluminescence immunoassays. Th9 and Th17 cells were analyzed using flow cytometry. The expression of Foxo1, IRF-4, RORc, IL-9, and IL-17 mRNA was examined using real-time PCR, and IL-9 and IL-17 protein levels were measured using enzyme-linked immunosorbent assay.ResultsTh9, Th17, and characteristic cytokines IL-9 and IL-17 in the GD-untreated group were significantly higher than those in the control and remission groups. The above indexes significantly decreased in the remission group, with the levels in the TRAb− remission group being similar to those in the normal group, while in the TRAb+ remission group, levels were differentially increased. TRAb titer was positively correlated with the levels of Th9, Th17, and their functional cytokines.ConclusionsTh9 and Th17 cells may be involved in the pathogenesis and disease outcome of GD, which could provide a new direction for developing immunotherapy for patients with GD.
Galectin-9 contributes to the pathogenesis of atopic dermatitis via T cell immunoglobulin mucin-3
Authors: Su Wenxing , Zhang Ji , Yang Shun , Tang Minhui , Shen Yu , Liu Cuiping , Ji Jiang , Maurer Marcus , Jiao Qingqing 
Published: July 2022

BackgroundAtopic dermatitis (AD), a common type 2 inflammatory disease, is driven by T helper (TH) 2/TH22polarization and cytokines.Galectin-9 (Gal-9),viaits receptor T cell immunoglobulin- and mucin-domain-containing molecule-3 (TIM-3), can promote TH2/TH22 immunity. The relevance of this in AD is largely unclear.ObjectivesTo characterize the role of TIM-3 and Gal-9 in the pathogenesis of AD and underlying mechanisms.MethodsWe assessed the expression of Gal-9 and TIM-3 in 30 AD patients, to compare them with those of 30 healthy controls (HC) and to explore possible links with disease features including AD activity (SCORAD), IgE levels, and circulating eosinophils and B cells. We also determined the effects of Gal-9 on T cells from the AD patients.ResultsOur AD patients had markedly higher levels of serum Gal-9 and circulating TIM-3-expressing TH1 and TH17 cells than HC. Gal-9 and TIM-3 were linked to high disease activity, IgE levels, and circulating eosinophils and/or B cells. The rates of circulating TIM-3-positive CD4+cells were positively correlated with rates of TH2/TH22 cells and negatively correlated with rates of TH1/TH17 cells. Gal-9 inhibited the proliferation and induced the apoptosis of T cells in patients with AD, especially in those with severe AD.ConclusionOur findings suggest thatGal-9,viaTIM-3, contributes to the pathogenesis of AD by augmenting TH2/TH22 polarization through the downregulation of TH1/TH17immunity. This makes Gal-9 and TIM-3 interesting to explore further, as possible drivers of disease and targets of novel AD treatment.
Hurricane and Typhoon Storm Wind Resolving NOAA NCEI Blended Sea Surface Wind (NBS) Product
Authors: Saha Korak , Zhang Huai-Min 
Published: July 2022

Improving forecasts of storms and hurricanes and their potential impacts is highly important to public safety, economic security, commerce, and community infrastructure. One key element of forecast improvement is more accurate and increased spatial–time coverage of observational data for model calibration, quality control and initialization, and/or data assimilation. The National Oceanic and Atmospheric Administration (NOAA) has been producing a global gridded 0.25° and 6-hourly sea surface winds product that has wide applications in marine transportation, marine ecosystem and fisheries, offshore winds, weather and ocean forecasts, and other areas. The NOAA National Centers for Environmental Information (NCEI) Blended Sea winds (NBS) v1.0 product is generated by blending observations from multiple sources (satellites), including scatterometers and microwave radiometers/imagers. However, these sensors do not provide accurate observations of intensive high-speed hurricane winds because their signals saturate in very high winds or degrade in the presence of rain. Recent advancements in satellite wind retrievals revealed that the L-band (1.42 GHz) instrument on the Soil Moisture Active Passive (SMAP) satellite and the AMSR2 All-Weather channel (~6.9 GHz) can provide accurate hurricane winds of up to 65 m/s (145 MPH) without being affected by rain; these data are incorporated in a new version of the Blended Sea Winds, NBS v2.0, using a multi-sensor data fusion technique based on random errors, enabling it to resolve very high winds, especially along the eyewalls of tropical cyclones and hurricanes. NBS v2.0 provides both a long-term record of 30+ years retrospectively since July 1987 and a near-real-time mode with 1-day latency.
Thermophysical Property Measurements of Refractory Oxide Melts With an Electrostatic Levitation Furnace in the International Space Station
Authors: Ishikawa Takehiko , Paradis Paul-François , Koyama Chihiro 
Published: July 2022

Due to their high melting temperatures and the risk of contamination from the crucibles, molten oxides which melting temperatures are above 2000 °C can hardly be processed using conventional methods. This explains that their thermophysical properties are very scarce. Containerless methods with gas flows have been developed and several thermophysical properties such as density, surface tension, and viscosity have been reported. However, the gas flow has detrimental side effects such as deformation of the sample and induction of internal flows in the molten sample, which affect the accuracy of the measurements. The electrostatic levitation furnace onboard the International Space Station (ISS-ELF), which utilizes the Coulomb force to levitate and melt samples in microgravity, has several advantages for thermophysical property measurements of refractory oxide melts. Levitation without a gas flow coupled to a reduced gravity environment minimizes the required levitation (positioning) force and reduces the deformation as well as the internal flow. This report briefly introduces the ISS-ELF facility and the thermophysical property measurement methods. The measured density, surface tension, and viscosity of molten Al2O3 are then presented and compared with the ones obtained by other methods. Finally, the measured data of refractory oxides whose melting temperatures are above 2,400 °C are summarized.