bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clonally Expanded B Cells in Multiple Sclerosis Bind EBV EBNA1 and GlialCAM
Authors: Lanz Tobias V , Brewer R Camille , Ho Peggy P , Jude Kevin M , Fernandez Daniel , Moon Jae-Seung , Fernandes Ricardo A , Gomez Alejandro M , Nadj Gabriel-Stefan , Bartley Christopher M , Schubert Ryan D , Hawes Isobel A , Vazquez Sara E , Iyer Manasi , Zuchero J Bradley , Teegen Bianca , Dunn Jeffrey E , Lock Christopher B , Kipp Lucas B , Cotham Victoria C , Ueberheide Beatrix M , Aftab Blake T , Anderson Mark S , DeRisi Joseph L , Wilson Michael R , Bashford-Rogers Rachael JM , Platten Michael , Garcia K Christopher , Steinman Lawrence , Robinson William H 
Published: January 2022

Abstract

Multiple sclerosis (MS) is a heterogenous autoimmune disease in which autoreactive lymphocytes attack the myelin sheath of the central nervous system (CNS). B lymphocytes in the cerebrospinal fluid (CSF) of MS patients contribute to inflammation and secrete oligoclonal immunoglobulins. Epstein-Barr virus (EBV) infection has been linked to MS epidemiologically, but its pathological role remains unclear. Here we demonstrate high-affinity molecular mimicry between the EBV transcription factor EBNA1 and the CNS protein GlialCAM, and provide structural and in-vivo functional evidence for its relevance. A cross-reactive CSF-derived antibody was initially identified by single-cell sequencing of the paired-chain B cell repertoire of MS blood and CSF, followed by protein microarray-based testing of recombinantly expressed CSF-derived antibodies against MS-associated viruses. Sequence analysis, affinity measurements, and the crystal structure of the EBNA1-peptide epitope in complex with the autoreactive Fab fragment allowed for tracking the development of the naïve EBNA1-restricted antibody to a mature EBNA1/GlialCAM cross-reactive antibody. Molecular mimicry is facilitated by a post-translational modification of GlialCAM. EBNA1 immunization exacerbates the mouse model of MS and anti-EBNA1/GlialCAM antibodies are prevalent in MS patients. Our results provide a mechanistic link for the association between MS and EBV, and could guide the development of novel MS therapies.
Identification of stressors and quantification of stress among veterinary students in India using a cross-sectional questionnaire survey
Authors: Grakh Kushal , Jadhav Vijay Jayawant , Panwar Diksha , Khurana Rajesh , Bangar Yogesh Chanderkant , Pandita Sakshi , Yadav Dheeraj 
Published: January 2022

Abstract
As an intensive professional training program veterinary education presents high academic and non-academic stressors to students. Identifying the stressors responsible and measuring the stress among veterinary students can prove useful to design any coping strategies to safeguard the mental health of students. The study aimed to identify the responsible stressors, measure the level of stress caused by each kind of stressor, the relation of stress with variables such as gender and family income. For this, 44 items based cross-sectional questionnaire survey was designed and distributed among veterinary students of India. On statistical analysis, the questionnaire yielded high internal consistency. More than 95% of students were found to be under stress. Maximum students reported the highest stress due to academic-related stressors. Female students reported high overall stress, academic stress, and intrapersonal and interpersonal-related stress than male students. Students with lower family incomes experienced more overall stress as well as family responsibilities-related stress. Gender, family income, academic stressors, intrapersonal, interpersonal, and conflict-related stressors, and social activities-related stressors positively predicted the overall stress among the students and thus these factors should be given special emphasis while designing any stress combating or reducing measures for veterinary students of India.
The effect of adding natural materials waste on the mechanical properties and water absorption of epoxy composite using grey relations analysis
Authors: Khalaf Ahmad A. , Abed Salwa A. , Alkhfaji Saad Sami , Al-Obaidi Mudhar A. , Hanon Muammel M. 
Published: January 2022

Recently, there has been a tendency for scientific studies to deal with natural materials as fillers and reinforcement for polymer composites, which are used in many different applications due to their environmentally friendly properties when compared to synthetic materials. The current study aims to preserve the environment by dealing with natural materials and their influence on the mechanical properties and water absorption property of the polymer composites. In this study, epoxy composites were produced from local natural sourced non-hazardous raw natural materials using grey relational analysis (GRG). The materials used for fabrication include micro-filler of pollen palm 50 μm, seashell 75 μm and epoxy resin. Nine different composites were prepared using pollen palm and seashell as reinforcement material by varying the wt % of the micro-filler. Rule of the mixture was used for formulation and wt % of (0.5, 1 and 1.5) % reinforcement and 99.5, 99 and 98.5 % epoxy (binder) were used for composites. Grey relational analysis was conducted in order to scale the multi-response performance to a single response. The results indicate that optimum performance can be achieved with the addition of 1.5 wt % micro-filler of seashell, which achieved the first rank, while the second rank achieved by 0.5 wt % micro-filler of palm pollen and seashell when compared to other composites. The addition of micro-fillers has improved greatly the mechanical properties of epoxy composites. The loading of micro-fillers has influenced the water absorption property of composites based epoxy in ascending order
Possibilities of colecalciferol therapy for tension type headache combined with hypovitaminosis D
Authors: Koloskova A. A. , Vorob’eva O. V. 
Published: January 2022

Introduction. Modern studies indicate the therapeutic effect of vitamin D (VD) in chronic pain conditions, but there is no data on the use of VD in chronic tension type headache (CTTH) treatment.Objective: comparative evaluation of the effectiveness of various options for preventive treatment of CTTH: the use of colecalciferol, standard therapy of the disease and a combination of these methods.Materials and methods. 125 women with CTTH and hypovitaminosis D were treated in the study. The frequency, duration and intensity of headache (HF, HD and HI, respectively), HIT index, frequency of analgesics taking, pericranial muscle dysfunction were evaluated. The VD level was measured by the concentration of 25-hydroxyvitamin D [25(OH)D]. The patients received differentiated therapy for 16 weeks: group 1 – colecalciferol, group 2 – amitriptyline, group 3 – a combination of these medicines. Results. In group 1, HF, HD, HIT-index and the frequency of analgesics taking decreased, all p ≤ 0.001. In groups 2 and 3, the values of all CTTH-parameters decreased, all p < 0.05. HF, HIT-index and the frequency of analgesics taking in group 3 decreased more than in group 2, all p >< 0.017. In women who had VD deficiency before treatment, the decrease in HF from the initial one was 36% in group 1, 55% in group 2, and 74% in group 3. In patients who had VD deficiency before treatment, the effectiveness of therapy in group 3 was higher than in group 2: 74% vs. 55%, respectively, p >< 0.001.Conclusions. Treatment of hypovitaminosis D in women with CTTH is accompanied by a decrease in HF, the effect of headache on the quality of life of patients and the frequency of taking analgesics. In CTTH and VD deficiency normalization of 25(OH)D level contributes to higher efficiency of standard therapy.
Asthenia and fatigue in hyperammonemia: etiopathogenesis and methods of correction
Authors: Plotnikova E. Yu. , Sinkova M. N. , Isakov L. K. 
Published: January 2022

Asthenia and fatigue are the most common syndromes in patients with liver disease, which significantly affects their quality of life. The prevalence of fatigue in chronic liver diseases is from 50% to 85%. While some progress has been made in understanding the processes that can cause fatigue in general, the underlying causes of fatigue associated with liver disease remain not well understood. In particular, many data suggest that fatigue associated with liver disease likely results from changes in neurotransmission in the brain against the background of hyperammonemia. Hyperammonemia is a metabolic state characterized by an increased level of ammonia, a nitrogen-containing compound. The present review describes hyperammonemia, which is likely important in the pathogenesis of fatigue associated with liver disease. Ammonia is a potent neurotoxin, its elevated blood levels can cause neurological signs and symptoms that can be acute or chronic, depending on the underlying pathology. Hyperammonemia should be recognized early, and immediately treated to prevent the development of life-threatening complications, such as, swelling of the brain and coma. The article gives pathophysiological mechanisms of influence of hyperammonemia on state of psychovegetative status of patients with liver diseases, also lists basic principles of treatment. A significant part of the article is devoted to L-ornithine-L-aspartate, which is effective in asthenia and fatigue to reduce the level of hyperammonemia through a variety of well-studied mechanisms in chronic liver diseases.
Assessment of the temporomandibular joint condition using two-dimensional ultrasound scanning and doppler ultrasonography methods in patients with chronic inflammatory periodontal diseases
Authors: Chapanova E. M. , Ikonnikova M. A. , Ikonnikov G. G. , Ermolev S. N. , Yanushevich O. O. , Merzhoev I. O. , Khitrov A. N. , Gryzunova G. K. , Chereshneva Y. V. , Ferzauli A. N. , Gatagazheva Z. M. , Tachieva B. I. 
Published: January 2022

Introduction. Currently, dentists are increasingly detecting changes in the temporomandibular joint in patients with chronic inflammatory periodontal disease.Aim of the study. To carry out a comprehensive dynamic assessment of the temporomandibular joint (TMJ) condition and the registration of regional blood flow using two-dimensional ultrasound scanning to improve the efficiency of diagnostics of inflammatory periodontal diseases.Materials and methods. The study included 2 groups of patients: group 1 (control) consisted of 20 volunteers aged 20–25; Group 2 consisted of 52 people aged 25–45 years with moderate chronic periodontitis. For TMJ ultrasound and Doppler ultrasound, a portable ultrasound scanner LogicScan 128 with an HL-10 linear ultrasound transducer with an operating frequency of 5 to 12 MHz was used.Results and discussion. During ultrasound examination of the temporomandibular joint and measuring the size of the joint space in patients with moderate chronic periodontitis in a state of relative physiological rest, the following values were obtained: in the anterior region – 2.3 ± 0.5 mm; in the upper section – 1.6 ± 0.6 mm; in the posterior section – 1.8 ± 0.3 mm. We also measured the area of the temporomandibular joint disc in various positions. According to ultrasound data, an increase in the size of the joint space from 12.2 to 16.1% and an increase in the area of the articular disc by 17.1 to 36.7% were found in patients with chronic periodontitis. When assessing the trajectory of the articular track, motion delay and joint wedging are determined. In addition, in the color Doppler mapping (CDM) mode, the speed and index indicators of Doppler ultrasonography of the external carotid and temporal arteries were calculated.Conclusions. Modern diagnostic methods of ultrasound and Doppler mapping, assessing the hemodynamics and functional state of the TMJ, allow early diagnosis of changes in order to prevent the development of TMJ disorders in patients with chronic inflammatory periodontal diseases.
Assessment of the vitamin d levels effect on the course of the first trimester of pregnancy
Authors: Kovalevа I. V. , Bakleicheva M. O. , Bespalova O. N. 
Published: January 2022

Introduction. The course of physiological pregnancy is provided by many complementary factors. Thus, a deficiency in one of the links of the metabolic network contributes to the development of an imbalance in the work of the whole organism, which ensures the growth and development of the embryo from the first days of gestation. It has been proven that vitamin D can act as an immune regulator during implantation, providing a protective effect in whole period of pregnancy.Aim. The aim of this study is assessed the features of the course of pregnancy in patients with different levels of vitamin D in the blood in the first trimester.Materials and methods. A prospective multicenter randomized study was conducted in the North-West region of the Russian Federation among 88 pregnant women in the first trimester of gestation (up to 13 weeks). All patients were divided into 3 groups depending on the initial level of vitamin D (group 1–14 women with a 25(OH)D < 10 ng/ml, group 2–62 pregnant women from 10 to 30ng/ml, group 3–12 pregnant women with a vitamin D content > 30 ng/ml).Results. In group 1.86% of patients with severe vitamin D deficiency were diagnosed with threatened miscarriage, which is significantly higher than in group 3 (85.7% and 33.3%, χ2 = 7.490, p = 0.007). At the same time, retrochorial hematoma in group 1 occurred 3.5 times more often than in group 3 (57.1% and 16.67%, respectively, χ2 = 4.473, p = 0.035). Subsequently, every 4th woman from the group with vitamin D deficiency gave birth earlier than expected, which was not observed among patients from group 3 (25%, 0%, χ2 = 1.231, p = 0.268).Conclusion. Prescribing cholecalciferol vitamin replacement therapy as part of complex preserving therapy for threatening miscarriage, followed by monitoring its blood level and deviating from normal parameters, contributing to a favorable course of pregnancy and improving perinatal outcomes.
Prediction of the transsphenoidal endoscopic adenomectomy results in patients with cushing’s disease
Authors: Kuritsyna N. V. , Tsoy U. A. , Cherebillo V. Yu. , Paltsev A. A. , Tsiberkin A. I. , Grineva E. N. 
Published: January 2022

Introduction. Transsphenoidal adenomectomy (TSA) is the method of choice in the treatment of Cushing’s disease (CD), but remission of hypercorticism cannot be achieved in all patients. The search for predictors of CD remission after TSA remains to be an important challenge in the endocrinology today.Aim. To study the preoperative and postoperative data of patients with CD to identify the predictors of hypercorticism remission after TSA.Materials and methods. 101 patients with confirmed CD after TSA were included. One year after operation all patients were examined for the presence of hypercorticism remission and divided into two groups: with CD remission and its absence. In both groups’ preoperative pituitary magnetic resonance imaging (MRI) data, the results of preoperative high dose dexamethasone suppression test (HDDST) and the results of serum cortisol collected in the morning 2–3 days after surgery were compared.Results. One year after TSA, CD remission was confirmed in 63 patients (62.4%), whereas in 38 patients (37.6%) hypercortisolism persisted. Favorable predictors of CD remission were: the adenoma size > 3 mm without the invasive growth according to pituitary MRI (specificity 82.4%, sensitivity 82.8%), serum cortisol suppression ≥ 74% in preoperative HDDST (specificity 81.5%, sensitivity 86.3%), morning serum cortisol level in 2–3 days after surgery ≤ 388 nmol/l (specificity 79.3%, sensitivity 97.4%).Conclusions. Pituitary MRI data, the results of preoperative HDDST and morning serum cortisol in the 2–3 days after surgery can be used as predictors of CD remission.
The role of non-drug methods of increasing adherence to the treatment of patients with arterial hypertension in a polyclinic
Authors: Simenyura S. S. , Sizova Zh. M. 
Published: January 2022

Introduction. Arterial hypertension (AH) is one of the important high-risk factors for the development of heart diseases, but its diagnosis and treatment does not require high technologies and substantial costs. However, the asymptomatic course of AH combined with a low level of medical literacy contributes to low adherence to treatment.Aim. To study the indirect impact of remote blood pressure monitoring (RBPM), the introduction of educational materials (EMs) in comparison with self-monitoring of blood pressure (SMBP) on adherence of patients with AH to outpatient treatment.Materials and methods. 60 subjects with an established fact of partial or complete refusal to use antihypertensive therapy were enrolled in the study and randomized into four equal and comparable groups: 1 – SMBP, 2 – RBPM, 3 – SMBP + EMs, 4 – RBPM + EMs. The impact of using RBPM was assessed by comparing blood pressure values in groups 1 and 2; the impact of EM – by comparing the results of questionnaire surveys in groups 1 and 2 and groups 3 and 4.Results and discussion. Additional training of patients with AH contributed to a twofold increase in adherence in using SCAD and RBPM. The average systolic blood pressure in groups 1 and 2 was 134 mm Hg, in groups 3 and 4 – 129 mm Hg; diastolic blood pressure in groups 1 and 2 – 78.5 mm Hg, in groups 3 and 4 – 77.5 mm Hg.Conclusion. The maximum impact on adherence to AH treatment can be achieved with the introduction of a set of measures. However, the introduction of RBPM has a number of limitations associated with the additional economic burden and the lack of an approved methodology. However, the provision of treatment and prevention facilities with EMs does not require additional development of methodology and is significantly cheaper than the cost of modern blood pressure monitors.
Intellectual decline in patients with schizophrenia with comorbid type ii diabetes mellitus and alcoholism
Authors: Kostyuk G. Р. , Cherepakhin D. I. , Aronov P. V. , Belskaya G. N. , Nikiforov I. A. 
Published: January 2022

Comorbid conditions in general psychopathological practice need equally research in the field of psychiatry and narcology, as well as the development of issues of social practice in relation to mental patients. There is an opinion that comorbid mental pathologies are even more common than “pure” forms of diseases. In most cases of comorbid conditions, the medical community increasingly encounters clinical situations where “classic” symptoms and syndromes are deformed, mutually intertwined and, superimposed on the actual social situation of the patient, acquire an “unreal fancy character”.Schizophrenia remains one of the most urgent problems at the stage of modern psychiatry formation. Up to date there are 1.1% of men and 1.9% of women in the general population of patients. Schizophrenic spectrum disorders are often combined with a number of chronic pathologies that increase the negative impact on the neuro-cognitive sphere of a person. One of the main problems of modern urbanized society is type II diabetes and alcoholism. By increasing the negative impact on a person’s cognitive abilities, they accelerate the process of disintegration of personality and its social functioning. The intellectual level of patients with those chronic diseases that require patients to actively and consciously participate in the treatment process and social functioning can significantly affect the patient’s ability to learn, independently manage the disease, establish a high level of compliance and, as a result, the effectiveness of therapy. An attentive study of the issue of the state of intelligence of patients with comorbid pathology will lead to an improvement in the patient’s social adaptation, a more careful attitude to their somatic health and reduce the risk of disability of the able-bodied population.
Defluoridation performance comparison of aluminum hydroxides with different crystalline phases
Authors: Gai Wei-Zhuo , Zhang Shi-Hu , Yang Yang , Sun Kexi , Jia Hong , Deng Zhen-Yan 
Published: January 2022

Abstract
Aluminum hydroxide is an eye-catching and extensively researched adsorbent for fluoride removal and its defluoridation performance is closely related to the preparation method and crystalline phase. In this research, the defluoridation performances of aluminum hydroxides with different crystalline phases are compared and evaluated in terms of fluoride removal capacity, sensitivity to pH values and residual Al contents after defluoridation. It is found that the defluoridation performance of different aluminum hydroxides follows the order of boehmite &gt; bayerite &gt; gibbsite. The fluoride adsorption on aluminum hydroxides follows the pseudo-second-order kinetic model and Langmuir isotherm model, and the maximum defluoridation capacities of boehmite, bayerite and gibbsite are 42.08, 2.97 and 2.74 mg m−2, respectively. The pH values and FTIR analyses reveal that the ligand exchange between fluoride and surface hydroxyl groups is the fluoride removal mechanism. Different aluminum hydroxides have different surface hydroxyl group densities, which results in the different defluoridation capacities. This work provides a new idea to prepare aluminum hydroxide with outstanding defluoridation performance.
Face Proportions, and Analysis of Maxillary Anterior Teeth and Facial Proportions in a Thai Population
Authors: Wadud Asikul , Kitisubkanchana Jira , Santiwong Peerapong , Srithavaj M.L. Theerathavaj 
Published: January 2022

Background:
Esthetics is the primary consideration for patients looking for prosthetic treatment. Determining the facial types of Thai populations is important because most studies conducted in Caucasian populations mention their facial types, and describe the gender comparisons, and GP of the teeth.

Objectives:
This study compared facial proportions, analyzed the maxillary anterior teeth proportions and determined the relationships between teeth with facial measurements using the Golden Proportion (GP) and Golden Standard (GS) in a Thai population.

Methods:
A total of 200 students (18-35 years) with well-aligned maxillary anterior teeth participated in this study. Face heights and widths were measured from the face using digital calipers and faces were classified as narrow, average, and broad. Teeth and face proportions were compared with GP and GS. One-way ANOVA was performed to compare maxillary anterior teeth proportions and facial proportions with different face types (α= 0.05). Pearson’s correlation was used (α= 0.05) to determine the correlation between facial proportion and teeth dimension among different facial types.

Results:
The findings showed 82.50% narrow face, 13.50% average, and 4% broad face among Thai subjects. No GP or GS was found in the face and anterior teeth, but teeth and face proportions other than GP and GS were found. No significant correlation was found between the Width of the Central Incisor (WCI) with interpupillary distance and alar width.

Conclusion:
In this study, the GP was not found in the facial and teeth proportion. The data obtained from this study may help to provide guidelines for prosthetic and orthodontic management and help to select the proper size and form of the maxillary anterior teeth to achieve proper esthetic outcomes in the Thai population.