bluuebunny/crossref_metadata_2025_split huggingface dataset:
The hydrogenosome of Trichomonas vaginalis
Authors: Tachezy Jan , Makki Abhijith , Hrdý Ivan 
Published: May 2022

AbstractThis review is dedicated to the 50th anniversary of the discovery of hydrogenosomes by Miklós Müller and Donald Lindmark, which we will celebrate the following year. It was a long journey from the first observation of enigmatic rows of granules in trichomonads at the end of the 19th century to their first biochemical characterization in 1973. The key experiments by Müller and Lindmark revealed that the isolated granules contain hydrogen‐producing hydrogenase, similar to some anaerobic bacteria—a discovery that gave birth to the field of hydrogenosomes. It is also important to acknowledge the parallel work of the team of Apolena Čerkasovová, Jiří Čerkasov, and Jaroslav Kulda, who demonstrated that these granules, similar to mitochondria, produce ATP. However, the evolutionary origin of hydrogenosomes remained enigmatic until the turn of the millennium, when it was finally accepted that hydrogenosomes and mitochondria evolved from a common ancestor. After a historical introduction, the review provides an overview of hydrogenosome biogenesis, hydrogenosomal protein import, and the relationship between the peculiar structure of membrane translocases and its low inner membrane potential due to the lack of respiratory complexes. Next, it summarizes the current state of knowledge on energy metabolism, the oxygen defense system, and iron/sulfur cluster assembly.
Effects of dietary nucleotides on growth performance, immune response, intestinal morphology and disease resistance of juvenile largemouth bass, Micropterus salmoides
Authors: Chen Xiao‐chun , Huang Xiao‐quan , Tang Yi‐wen , Zhang Lei , Lin Feng 
Published: May 2022

AbstractAn 8 week feeding trial was carried out to investigate the effects of dietary nucleotides on growth performance, intestinal morphology, immune response and disease resistance of juvenile largemouth bass, Micropterus salmoides. Five grades of dietary nucleotide levels were designed as 0, 0.2, 0.4, 0.8 and 1.2 g kg−1, respectively. Each group had 3 replicates, with 40 fish in each replicate. After the feeding experiment, 15 fish from each tank were infected with Aeromonas hydrophila for 14 days. The results indicated that fish fed the diets containing 0.4, 0.8 and 1.2 g kg−1 nucleotides had higher growth performance and feed utilization than those fed the control diet. Nonetheless, there were no significant differences in survival between all the groups, although fish fed the diets with all‐level nucleotides obtained higher survival than those fed the control diet. Dietary nucleotides significantly affected the superoxide dismutase, acid phosphatase and catalase activities in serum but not the malondialdehyde content. Fish fed the 0.4 g kg−1 nucleotide diets had the highest fold height, enterocyte height and muscular layer thickness significantly. The average mortality of largemouth bass infected with A. hydrophila was significantly influenced by dietary nucleotides. The mortality was significantly higher in the control group (91.11%) and 0.02% nucleotide group (73.11%) followed by the other groups and lowest in the 0.8 g kg−1 nucleotide group. In summary, dietary 0.4–0.8 g kg−1 nucleotides promoted growth performance, enhanced immunity and improved intestinal morphology and disease resistance of largemouth bass.
Relative validity and reproducibility of the CyFFQ semiquantitative food frequency questionnaire for assessing dietary intake in Cypriot adults
Authors: Philippou Elena , Demetriou Christiana A. , Loucaides George , Solomonidou Nastazia , Critselis Elena , Polykarpou Maria , Sioulis Spyros , Hadjisavvas Andreas , Kyriacou Kyriacos 
Published: May 2022

AbstractBackgroundAssessment of dietary intake is fundamental for evaluating the interrelationships between diet and disease. The present study aimed to develop and validate the semiquantitative Cypriot food frequency questionnaire (CyFFQ).MethodsA 171‐item paper‐and‐pencil semiquantitative interview‐administered FFQ was developed, including local foods and culturally specific meals commonly consumed among Cypriot adults. FFQ reproducibility was assessed by comparing the energy‐adjusted daily macro‐ and micronutrients intake at baseline (FFQ1) and 1 year later (FFQ2) using a Wilcoxon matched pairs signed rank test and intraclass correlation coefficients (ICCs) in a random sample of Cypriot adults. FFQ relative validity was evaluated by comparing the intake as estimated by FFQ2 with that obtained from the average of three 24‐h recalls taken over the year between FFQ1 and FFQ2. Associations between nutrient intakes estimated using FFQ2 and the 24‐h recalls were assessed using Spearman rank correlation and Bland–Altman plots were used to assess agreement between the FFQ and the 24‐h recalls.ResultsAmong eligible participants, 68 (78%) completed the study (44.1% males, aged 30.5–47.5 years). The energy‐adjusted intakes of macro‐ and micronutrients did not significantly differ between the two FFQs, excluding magnesium. The FFQ2 and the averaged 24‐h recalls were significantly correlated for most macro‐ and micronutrients. The median (interquartile) ICC for all macro‐ and micronutrients was 0.46 (0.38–0.52) (p < 0.05). Agreement was satisfactory (>30%) for most micro‐ and macronutrients. Bland–Altman plots also confirmed good agreement between the two methods.ConclusionsThe CyFFQ is a valid and reliable tool for assessing dietary consumption in Cypriot adults.
Protein interactome and cell‐type expression analyses reveal that cytoplasmic FMR1‐interacting protein 1 (CYFIP1), but not CYFIP2, associates with astrocytic focal adhesion
Authors: Ma Ruiying , Pang Kaifang , Kang Hyojin , Zhang Yinhua , Bang Geul , Park Sangwoo , Hwang Eunha , Ryu Jae Ryun , Kwon Yujin , Kang Hyae Rim , Jin Chunmei , Kim Yoonhee , Kim Su Yeon , Kwon Seok‐Kyu , Kim Doyoun , Sun Woong , Kim Jin Young , Han Kihoon 
Published: May 2022

AbstractThe two members of the cytoplasmic FMR1‐interacting protein family, CYFIP1 and CYFIP2, are evolutionarily conserved multifunctional proteins whose defects are associated with distinct types of brain disorders. Even with high sequence homology between CYFIP1 and CYFIP2, several lines of evidence indicate their different functions in the brain; however, the underlying mechanisms remain largely unknown. Here, we performed reciprocal immunoprecipitation experiments using CYFIP1‐2 × Myc and CYFIP2‐3 × Flag knock‐in mice and found that CYFIP1 and CYFIP2 are not significantly co‐immunoprecipitated with each other in the knock‐in brains compared with negative control wild‐type (WT) brains. Moreover, CYFIP1 and CYFIP2 showed different size distributions by size‐exclusion chromatography of WT mouse brains. Specifically, mass spectrometry‐based analysis of CYFIP1‐2 × Myc knock‐in brains identified 131 proteins in the CYFIP1 interactome. Comparison of the CYFIP1 interactome with the previously identified brain region‐ and age‐matched CYFIP2 interactome, consisting of 140 proteins, revealed only eight common proteins. Investigations using single‐cell RNA‐sequencing databases suggested non‐neuronal cell‐ and neuron‐enriched expression of Cyfip1 and Cyfip2, respectively. At the protein level, CYFIP1 was detected in both neurons and astrocytes, while CYFIP2 was detected only in neurons, suggesting the predominant expression of CYFIP1 in astrocytes. Bioinformatic characterization of the CYFIP1 interactome, and co‐expression analysis of Cyfip1 with astrocytic genes, commonly linked CYFIP1 with focal adhesion proteins. Immunocytochemical analysis and proximity ligation assay suggested partial co‐localization of CYFIP1 and focal adhesion proteins in cultured astrocytes. Together, these results suggest a CYFIP1‐specific association with astrocytic focal adhesion, which may contribute to the different brain functions and dysfunctions of CYFIP1 and CYFIP2.
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Cover Image for this issue: https://doi.org/10.1111/jnc.15410
Epidemiology of chronic inflammatory demyelinating polyradiculoneuropathy in The Netherlands
Authors: Broers Merel C. , de Wilde Marcel , Lingsma Hester F. , van der Lei Johan , Verhamme Katia M.C. , Jacobs Bart C. 
Published: May 2022

AbstractChronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is a rare but disabling disorder that often requires long‐term immunomodulatory treatment. Background incidence rates and prevalence and risk factors for developing CIDP are still poorly defined. In the current study, we used a longitudinal population‐based cohort study in The Netherlands to assess these rates and demographic factors and comorbidity associated with CIDP. We determined the incidence rate and prevalence of CIDP between 2008 and 2017 and the occurrence of potential risk factors in a retrospective Dutch cohort study using the Integrated Primary Care Information (IPCI) database. Cases were defined as CIDP if the diagnosis of CIDP was described in the electronic medical file. In a source population of 928 030 persons with a contributing follow‐up of 3 525 686 person‐years, we identified 65 patients diagnosed with CIDP. The overall incidence rate was 0.68 per 100 000 person‐years (95% CI 0.45‐0.99). The overall prevalence was 7.00 per 100 000 individuals (95% CI 5.41‐8.93). The overall incidence rate was higher in men compared to woman (IRR 3.00, 95% CI 1.27‐7.11), and higher in elderly of 50 years or older compared with people <50 years of age (IRR 17 95% CI 4‐73). Twenty percent of CIDP cases had DM and 9% a co‐existing other auto‐immune disease. These background rates are important to monitor changes in the frequency of CIDP following infectious disease outbreaks, identify potential risk factors, and to estimate the social and economic burden of CIDP.
Flexural Properties and Hardness of CAD‐CAM Denture Base Materials
Authors: Fouda Shaimaa M. , Gad Mohammed M. , Abualsaud Reem , Ellakany Passent , AlRumaih Hamad S. , Khan Soban Q. , Akhtar Sultan , al‐Qarni Faisal D. , Al‐Harbi Fahad A. 
Published: May 2022

AbstractPurposeTo compare flexural strength, elastic modulus, and surface hardness of computer aided design and computer aided manufacturing CAD‐CAM milled, 3D‐printed, and heat‐polymerized denture base resins.Materials and methodsA total of 120 specimens were fabricated from heat‐polymerized acrylic resin (HP), milled resin (Avadent and IvoCad), and 3D‐printed resin (ASIGA, FormLabs, and NextDent). The specimens were divided into 6 groups according to the type of denture base material (n = 20/material) (10/flexural properties and 10/hardness). Flexural strength and elastic modulus of the specimens were evaluated by 3‐point bending test and surface hardness by Vickers hardness test. To test flexural properties, the specimens were fabricated according to ISO 20795‐1:2013 standards (64 × 10 × 3.3 ± 0.2 mm). The dimensions for hardness test were 15 × 10 × 2.5 ± 0.2 mm. Scanning electron microscope was used to evaluate the surface morphology of the fractured specimens. The means and standard deviations were calculated, followed by one‐way ANOVA and Tukey post‐hoc test (α = 0.05).ResultsMilled resins showed significantly higher values for flexural strength, elastic modulus, and surface hardness, followed by HP and then 3D‐printed resins (p < 0.001). Within milled groups, flexural strength of AvaDent was significantly higher than IvoCad (p < 0.001), while elastic modulus and hardness didn't show significant difference. Within 3D‐printed resins, ASIGA showed the highest flexural strength and elastic modulus, insignificantly with FormLabs (p = 0.595) and significantly with NextDent (p = 0.008). ASIGA also showed significantly the highest hardness among the 3D‐printed groups. No significant difference was found between FormLabs and NextDent in flexural strength (p = 0.357), elastic modulus (p = 1.00), or surface hardness (p = 0.987).ConclusionCAD‐CAM milled resins had greater flexural properties and hardness compared to heat‐polymerized acrylic resin and 3D‐printed resins. Although 3D‐printed samples showed the lowest values of tested properties, the flexural strength and modulus were above clinically acceptable values.
Introversion and the frequency and intensity of daily uplifts and hassles
Authors: DeMeo Natasha N. , Smyth Joshua M. , Scott Stacey B. , Almeida David M. , Sliwinski Martin J. , Graham‐Engeland Jennifer E. 
Published: May 2022

AbstractObjectiveThere is reason to believe that introversion may relate to different patterns of negative and positive experiences in everyday life (“hassles” and “uplifts”), but there is little evidence for this based on reports made in daily life as events occur. We thus extend the literature by using data from ecological momentary assessments to examine whether introversion is associated with either the frequency or intensity of hassles and uplifts.MethodParticipants (N = 242) were community‐dwelling adults (63% Black, 24% Hispanic; ages 25‐65; 65% women) who completed baseline measures of personality and mental health, followed by reports of hassles and uplifts 5x/day for 14 days. We present associations between introversion and hassles/uplifts both with and without controlling for mood‐related factors (neuroticism, recent symptoms of depression, and anxiety).ResultsIntroversion was associated with reporting less frequent and less enjoyable uplifts, but not with overall hassle frequency or unpleasantness; exploratory analyses suggest associations with specific types of hassles.ConclusionsOur results expand understanding of the role of introversion in everyday experiences, suggesting an overall association between introversion and uplifts (but not hassles, broadly) in daily life. Better understanding of such connections may inform future research to determine mechanisms by which introversion relates to health.
The long and short of mistress relationships: Sex‐differentiated mate preferences reflect a compromise of mating ideals
Authors: Choy Bryan K. C. , Li Norman P. , Tan Kenneth 
Published: May 2022

AbstractObjectiveEvolved mate preferences have taken center stage in evolutionary psychology research, yet this literature has been fairly muted on mate preferences for extrapair partners. Here, we examined the mate preferences for mistress relationships (the traits that men prioritize in a mistress and mistresses prioritize in their male partners) and compared these preferences to those of short‐ and long‐term relationships.MethodIn two studies (NStudy 1a = 104, NStudy 1b = 191), we derived dimensions of mate preferences through exploratory factor analyses. In subsequent studies (NStudy 2 = 219, NStudy 3 = 101), we employed a budget allocation paradigm, where participants designed their ideal mates for different relationship types (short‐term, long‐term, and mistress relationships).ResultsWhereas men focused on fulfilling short‐term mating ideals (by prioritizing physical attractiveness) in a mistress relationship, women focused on fulfilling long‐term (but also some short‐term) mating ideals (prioritizing both physical attractiveness and social status) for a mistress relationship.ConclusionFindings indicate that mistress relationships reflect a compromise of men's and women's (conflicting) mating ideals and contribute to an understanding of relationships that are neither completely short‐ nor long‐term in nature.
Gender‐based cyberbullying: Understanding expected bystander behavior online
Authors: Herry Emily , Mulvey Kelly Lynn 
Published: May 2022

AbstractAs online access grows widespread, individuals may be increasingly subjected to cyberbullying, including cyber‐harassment targeting one's gender. Although bystander intervention can help stop bullying, little is known about factors that promote bystander intervention in instances of gender‐based cyberbullying. This study examines expected bystander responses to gender‐based cyberbullying with emerging adults from the United States (N = 373, Mage = 18.89, SD = .94). Hierarchical regressions demonstrated that women, those with prior experience as victims of gender‐based discrimination and those encountering male transgressors were more likely to expect that they would support a victim of gender‐based cyberbullying. Additionally, the better participants were at perceiving and understanding emotions, the less they expected that they would support the cyberbully and the more they expected they would support the victim of gender‐based cyberbullying.
Cost‐effectiveness of probiotics and fluoride varnish in caries prevention in preschool children
Authors: Rodriguez Gonzalo A. , Cabello Rodrigo A. , Borroni Catalina P. , Palacio Raul A. 
Published: May 2022

AbstractObjectivesThe purpose of this study was to evaluate the cost‐effectiveness of two preventive interventions aimed at increasing the proportion of caries‐free preschool children of low socioeconomic status using a decision analytic model.MethodsTwo scenarios were tested, one with a school milk program (SMP) and one without (non‐SMP). Fluoride varnish (FV) and a probiotic (PB) were compared to a do‐nothing alternative among children in public nurseries/schools over a 4‐year period. FV was applied biannually and a PB (Lactobacillus rhamnosus) added to milk powder prepared daily. A Markov decision tree model was utilized. Several sources of data were used to populate the model. Probabilistic and deterministic sensitivity analyses were performed, and a public provider perspective was used.ResultsIn the SMP scenario, PB was more effective and less costly than FV and, compared with do‐nothing, increased the proportion of caries‐free children by 14.5%, with a cost of USD 12.5 per child (June 2018). PB presented an incremental cost‐effectiveness ratio (ICER) or cost per extra caries‐free child of USD 86.2. In the non‐SMP scenario, both interventions were cost‐effective. FV (compared with do‐nothing) increased the percentage of caries‐free children by 8.3% with an ICER of USD 338.3 and PB (compared with FV) increased the effect by 6.2% with an ICER of USD 1400.2.ConclusionsThe findings showed that PB was most effective and less costly than FV in the SMP scenario only. This type of analysis and its results provide essential information for decision‐makers to improve the oral health of preschool children.
Integrative taxonomy and phylogeny of leafless Vanilla orchids from the South‐West Indian Ocean region reveal two new Malagasy species
Authors: Andriamihaja Cathucia F. , Botomanga Alemao , Misandeau Chantal , Ramarosandratana Aro V. , Grisoni Michel , Da Silva Denis , Pailler Thierry , Jeannoda Vololoniaina H. , Besse Pascale 
Published: May 2022

AbstractThe leafless Vanilla species complex from the South‐West Indian Ocean (SWIO) region has long been a taxonomic challenge, due to limited patterns of morphological differentiation and an absence of variation within chloroplast sequences. This complex includes seven known morphospecies: V. madagascariensis, V. bosseri, V. decaryana, and V. perrieri endemic to Madagascar, V. humblotii presumed as endemic to the Comoros Archipelago, but also present in Madagascar, V. roscheri from the East African coast, and V. phalaenopsis endemic to Seychelles. A previous population genetic study using microsatellite markers allowed us to distinguish, in addition to the five recognized Malagasy taxa, two other genetic clusters present in the East of the island. An integrative taxonomy approach was therefore conducted by combining microsatellite and morphological data used in the previous study with new data sets, and by adding ITS sequencing data, to validate the taxonomic level of these Malagasy genetic clusters and unravel phylogenetic relationships between SWIO species. As a result, based on phylogenetic, genotypic and morphological evidence, nine species were discriminated in the SWIO region, including seven in Madagascar, with two new eastern species. The leafless Vanilla group originated and diversified in Madagascar, from an ancestor of African descent, with three subsequent independent colonization events from Madagascar to the other territories of SWIO within the two main lineages (white versus yellow flower species). The new Malagasy species, V. allorgeae Andriamihaja & Pailler sp. nov., and V. atsinananensis Andriamihaja & Pailler sp. nov., are described and a new identification key is proposed.
Signal transducer and activator of transcription 3 cooperates with androgen receptor/cell cycle‐related kinase signalling pathway in the progression of hepatitis B virus infection and gender differences
Authors: Xu Yanhui , Qi Wenqian , Wang Xu , Zhang Yonggui , Han Liang , Shi Jingyi , Wang Guohua , Liu Jia , Duan Honglei , Cong Xianling , Zhao Ping , Zhou Changyu , Wang Jiangbin 
Published: May 2022

AbstractThe study aimed to investigate the role of androgen receptor (AR)/cell cycle‐related kinase (CCRK) signalling pathway in chronic hepatitis B virus (HBV) infection and gender differences, and the contribution of AR regulatory factor signal transducer and activator of transcription 3 (STAT3) in it. AR, CCRK, and phosphorylated STAT3 expressions in liver tissues of chronic HBV‐infected patients and non‐HBV controls were determined by western blot and compared between genders. The relationships of expression levels with serum HBV DNA levels, liver inflammation activity, and fibrosis score were analysed in chronic HBV‐infected patients. The relationships between expression levels of three proteins were also analysed. HBV‐infected patients had significantly higher expression levels of AR, CCRK, and p‐STAT3Tyr705 compared with controls (p < .01). The expression levels of AR, CCRK, and p‐STAT3Tyr705 in chronic HBV‐infected patients with severe inflammation were significantly higher than those with mild inflammation (p < .05). Expression levels in patients with heavier fibrosis (stage F4) were higher than in those with less fibrosis (stages F0–3) (p < .01). No gender differences were observed in AR, CCRK, and p‐STAT3Tyr705 levels in non‐HBV controls; higher levels were observed in HBV‐infected males than in HBV‐infected females (p < .05). AR, CCRK, and p‐STAT3Tyr705 levels in liver tissues positively correlated with each other (p < .0001) and with serum HBV DNA levels (p < .0001). In conclusion, in this study, we first found concordant over‐expression of AR, CCRK, and STAT3 in liver tissues of chronic HBV‐infected patients who have not yet developed HCC, significantly correlated with the severity of the disease and showed gender differences. STAT3 may be a potential therapeutic co‐target for chronic HBV infection.
Image, text and design: students' semiotic choices in nonfiction compositions
Authors: Moses Lindsey , Serafini Frank 
Published: May 2022

AbstractThis study investigates the ways in which first graders utilised a variety of semiotic resources when authoring nonfiction compositions during a nonfiction genre writing unit of study. Grounded in social semiotics and multimodality theories, the study uses a case study approach to explore the text, image and design resources used in students' multimodal compositions as a group followed by an in‐depth examination of two students' compositions. The data collection includes student compositions, video of classroom interactions and video recorded student composition/artefact elicitation interviews in which students discussed their composition process, authorial decision making and final composition. A multimodal approach to data analysis examines the semiotic resources used by the children, their meaning potential and purposes for inclusion in their nonfiction compositions. Findings suggest students drew upon a range of modalities, including visual images, graphic design features and movable text, in addition to written language to share their ideas and present their information through multimodal compositions. Implications include a need for curricular and instructional advancements with a greater focus on multimodal composing techniques to create inclusive spaces where students can more fully represent what they want to communicate.