bluuebunny/crossref_metadata_2025_split huggingface dataset:
Social networks respond to a disease challenge in calves
Authors: Burke Katharine C. , do Nascimento-Emond Sarah , Hixson Catherine L. , Miller-Cushon Emily K. 
Published: June 2022

AbstractChanges in network position and behavioral interactions have been linked with infectious disease in social animals. Here, we investigate the effects of an experimental disease challenge on social network centrality of group-housed Holstein bull dairy calves. Within group-housed pens (6/group) calves were randomly assigned to either a previously developed challenge model, involving inoculation with Mannheimia haemolytia (n = 12 calves; 3 calves/group) or a control involving only saline (n = 12 calves; 3 calves/group). Continuous behavioral data were recorded from video on pre-treatment baseline day and for 24 h following inoculation to describe social lying frequency and duration and all active social contact between calves. Mixed-model analysis revealed that changes in network position were related to the challenge. Compared to controls, challenged calves had reduced centrality and connectedness, baseline to challenge day. On challenge day, challenged calves were less central in the directed social contact networks (lower degree, strength and eigenvector centrality), and initiated contact (higher out-degree) with more penmates, compared to healthy calves. This finding suggests that giving rather than receiving affiliative social contact may be more beneficial for challenged calves. This is the first study demonstrating that changes in social network position coincide with an experimental challenge of a respiratory pathogen in calves.
A combination of scanning electron microscopy and broad argon ion beam milling provides intact structure of secondary tissues in woody plants
Authors: Hatano Tomohiro , Nakaba Satoshi , Horikawa Yoshiki , Funada Ryo 
Published: June 2022

AbstractThe secondary tissues of woody plants consist of fragile cells and rigid cell walls. However, the structures are easily damaged during mechanical cross-sectioning for electron microscopy analysis. Broad argon ion beam (BIB) milling is commonly employed for scanning electron microscopy (SEM) of hard materials to generate a large and distortion-free cross-section. However, BIB milling has rarely been used in plant science. In the present study, SEM combined with BIB milling was validated as an accurate tool for structural observation of secondary woody tissues of two samples, living pine (Pinus densiflora) and high-density oak wood (Quercus phillyraeoides), and compared with classical microtome cross-sectioning. The BIB milling method does not require epoxy resin embedding because of prior chemical fixation and critical point drying of the sample, thus producing a three-dimensional image. The results showed that xylem structures were well-preserved in their natural state in the BIB-milled cross-section compared with the microtome cross-section. The observations using SEM combined with BIB milling were useful for wide-area imaging of both hard and soft plant tissues, which are difficult to observe with transmitted electron microscopy because it is difficult to obtain sections of such tissues, particularly those of fragile reaction woods.
Genetic architecture of band neutrophil fraction in Iceland
Authors: Oskarsson Gudjon R. , Magnusson Magnus K. , Oddsson Asmundur , Jensson Brynjar O. , Fridriksdottir Run , Arnadottir Gudny A. , Katrinardottir Hildigunnur , Rognvaldsson Solvi , Halldorsson Gisli H. , Sveinbjornsson Gardar , Ivarsdottir Erna V. , Stefansdottir Lilja , Ferkingstad Egil , Norland Kristjan , Tragante Vinicius , Saemundsdottir Jona , Jonasdottir Aslaug , Jonasdottir Adalbjorg , Sigurjonsdottir Svanhvit , Petursdottir Karen O. , Davidsson Olafur B. , Rafnar Thorunn , Holm Hilma , Olafsson Isleifur , Onundarson Pall T. , Vidarsson Brynjar , Sigurdardottir Olof , Masson Gisli , Gudbjartsson Daniel F. , Jonsdottir Ingileif , Norddahl Gudmundur L. , Thorsteinsdottir Unnur , Sulem Patrick , Stefansson Kari 
Published: June 2022

AbstractThe characteristic lobulated nuclear morphology of granulocytes is partially determined by composition of nuclear envelope proteins. Abnormal nuclear morphology is primarily observed as an increased number of hypolobulated immature neutrophils, called band cells, during infection or in rare envelopathies like Pelger-Huët anomaly. To search for sequence variants affecting nuclear morphology of granulocytes, we performed a genome-wide association study using band neutrophil fraction from 88,101 Icelanders. We describe 13 sequence variants affecting band neutrophil fraction at nine loci. Five of the variants are at the Lamin B receptor (LBR) locus, encoding an inner nuclear membrane protein. Mutations in LBR are linked to Pelger-Huët anomaly. In addition, we identify cosegregation of a rare stop-gain sequence variant in LBR and Pelger Huët anomaly in an Icelandic eight generation pedigree, initially reported in 1963. Two of the other loci include genes which, like LBR, play a role in the nuclear membrane function and integrity. These GWAS results highlight the role proteins of the inner nuclear membrane have as important for neutrophil nuclear morphology.
In vivo enzymatic digestion of HRV 3C protease cleavage sites-containing proteins produced in a silkworm-baculovirus expression system
Authors: Xu Jian , Nakanishi Takafumi , Kato Tatsuya , Park Enoch Y. 
Published: June 2022

Abstract
Baculovirus expression vector system (BEVS) has been recognized as a potent protein expression system in engineering valuable enzymes and vaccines. Various fusion tags facilitate protein purification, leaving the potential risk to influence the target protein's biological activity negatively. It is of great interest to consider removing the additional tags using site-specific proteases, such as human rhinoviruses (HRV) 3C protease. The current study validated the cleavage activity of 3C protease in Escherichia coli and silkworm-BEVS systems by mixing the cell or fat body lysates of 3C protein and 3C site containing target protein in vitro. Further verification has been performed in the fat body lysate from co-expression of both constructs, showing remarkable cleavage efficiency in vivo silkworm larvae. We also achieved the glutathione-S-transferase (GST) tag-cleaved product of the VP15 protein from the White spot syndrome virus after purification, suggesting that we successfully established a coinfection-based recognition-and-reaction BEVS platform for the tag-free protein engineering.
Amidation of α-Amino Acids Using Dichloro(methyl)(3,3,3-trifluoropropyl)silane and Imidazole without Conventional Protection and Deprotection of α-Amino Group
Authors: Nobuta Tomoya , Morishita Honoka , Suto Yutaka , Yamagiwa Noriyuki 
Published: June 2022

AbstractAmidation of amino acids using dichloro(methyl)(3,3,3-trifluoropropyl)silane (MTFPSCl2) and imidazole is described. MTFPSCl2 activates the carboxy group and protects the α-amino group of amino acids. The amidation proceeded with 19 amino acids and 19 amines, including α-branched amines and anilines; the corresponding amino acid amides were synthesized in good-to-high yields (up to 96%) with low-to-no racemization.
Endoscopic Options for Moderate COPD, Chronic Bronchitis, and Uncontrolled Asthma
Authors: Herth Felix J.F. , Kontogianni Konstantina , Brock Judith 
Published: June 2022

AbstractUntil now, interventional therapies for patients with chronic obstructive pulmonary disease have been available in the form of lung volume reduction procedures as end-stage options. Currently, the range of indications is expanding to include earlier stages of the diseases. Lung denervation is available for moderate COPD, and patients with chronic bronchitis are being evaluated for endoscopic goblet cell ablation. Rheoplasty, metered spray cryo technique, and Karakoca resector balloon are used for this indication. But also, for patients with severe uncontrolled asthma, several techniques are available today. In addition to thermoplasty as a long-proven procedure, new and currently under investigation is the targeted lung denervation.Most of these techniques are currently being tested in large pivotal trials and it will soon become clear in which phenotype which technique will be used in the different forms and stages of obstructive diseases. The current paper presents the techniques and the currently available literature.
Isolated Headache as a Symptom of the Acute Coronary Syndrome in a Case following Myocardial Revascularization
Authors: Nanda Chinmaya , Mehta Yatin 
Published: June 2022

AbstractRetrosternal chest pain is the classical symptom of acute coronary syndrome (ACS). ACS sometimes presents with atypical symptoms and very rarely as headache as the only symptom. We present here a case where a patient who had undergone coronary artery bypass grafting presented with headache and on evaluation found to have complete occlusion of right coronary artery.
Infections Acquired During Venoarterial Extracorporeal Membrane Oxygenation Postcardiac Surgery in Children: A Retrospective Observational Study
Authors: Yagani Seshagiribabu , Singh Sarvesh Pal , Sahu Manoj Kumar , Choudhary Shiv Kumar , Chowdhury Ujjwal Kumar , Hote Milind Padmakar , Singh Ummed , Reddy Pradeep Ramakrishna , Panday Shivam 
Published: June 2022

Abstract
Introduction Extracorporeal membrane oxygenation (ECMO) is increasingly being used in refractory cardiac and pulmonary dysfunction as a rescue modality. The common indications for establishing venoarterial ECMO (VA-ECMO) support in children postcardiac surgery are failure to wean from cardiopulmonary bypass (CPB), postcardiotomy cardiogenic shock (PCCS), refractory pulmonary arterial hypertension, and as a bridge to recovery or transplant. The survival rate of children on VA-ECMO support is 45%. The most frequently encountered complications during VA-ECMO are bleeding, thrombosis, acute kidney injury, and infections. Among those, infections acquired during VA-ECMO lead to high morbidity and mortality. Hence, this study aimed to determine infection rates, causal microorganisms, and mortality risk factors in children developing an infection during VA-ECMO therapy.
Methods This retrospective observational study was conducted on 106 children under 14 years of age who underwent elective or emergent cardiac surgery (between 2016 and 2020) and required VA-ECMO support. Medical records were reviewed to collect the targeted variables and analyzed.
Results Out of 106 children, 49 (46.23%) acquired infections representing a prevalence of 46.23% and an infection rate of 186.4 episodes per 1,000 ECMO days. Prevalence and acquired infection rate/1,000 ECMO days were higher in the nonsurvivor group than in the survivor group (26.42 vs.19.81%) and (215.07 vs. 157.49), respectively. The bloodstream infection (BSI) and catheter-associated urinary tract infection (CAUTI) episodes were 53.04 and 68.19 per 1,000 ECMO days, and the ventilator-associated pneumonia (VAP) rate was 44.50 per 1,000 ventilator days. The mean preoperative admission duration, aortic cross-clamping duration, CPB duration (minutes), and vasoactive-inotropic score were higher in the nonsurviving children (p < 0.001). Similarly, prolonged mean ECMO duration was also found in the nonsurvivor group compared with the survivor group (p = 0.03).
Conclusion In our study, the prevalence of acquired infection during VA-ECMO was 46.23%. The incidence of BSI, CAUTI, and VAP per 1,000 ECMO days was higher in the nonsurvivor group than in survivors. Acinetobacter baumannii was the most common cultured gram-negative organism in VAP and BSI, with 67.65% Acinetobacter spp. resistant to carbapenems. CAUTI was predominately due to Candida species during VA-ECMO.
Dysglycemia in ICU Patients
Authors: Joshi Anshu , Mehta Yatin 
Published: June 2022

AbstractDysglycemia has emerged as a very common challenge in critically ill patients, especially with regard to current coronavirus disease 2019 pandemic. Prediabetes, poorly controlled diabetes, pharmaceutical intervention in intensive care unit (ICU) with glucocorticoids, catecholamines and other medicines, and stress response all contribute to dysglycemia in critically ill patients. Early identification and management are the key to prevent further complications. Patient prognosis in terms of clinical outcome, length of ICU stay, and in-hospital morbidity/mortality are adversely affected by patient's dysglycemic status. Apart from hyperglycemia, the other three important pillars of dysglycemia are discussed in this article. Synopsis of early intervention have been captured from India-specific practice guidelines. Important landmark trials have also been captured in this article to provide a clarity on certain aspects of managing dysglycemia in ICUs. Hence, this review article is an attempt to bring forth the salient aspects in diagnosing and managing dysglycemia in critical care settings.
Risk of Developing Acute Kidney Injury on the VA ECLS Circuit in Patients with Acute Decompensated Heart Failure
Authors: Reaz Shams , Loyaga-Rendon Renzo , Dickinson Michael G. , Lee Sangjin , Fitch Stephen J. , Marco Greg , Boeve Theodore , Langholz David E. , Kelty Catherine , Shrestha Nabin Manandhar , Leacche Marzia , Jovinge Stefan 
Published: June 2022

Abstract
Aims Venoarterial extracorporeal life support (VA ECLS) is a life-saving treatment with a high risk of mortality. Appropriate patient selection is critical for optimal patient outcomes. Acute kidney injury (AKI) is a common risk among VA ECLS patients, and more information is needed to understand how AKI affects the mortality risk of these patients. To do this, we examined acute decompensated heart failure (ADHF) patients supported by VA ECLS and compared their risk of developing AKI to a background population. VA ECLS has become an increasingly important tool to bridge or recover patients with severe ADHF as the primary indication of VA ECLS.
Methods and Results All VA ECLS patients from a single center were included. ADHF patients supported by VA ECLS were compared with the remaining VA ECLS cohort. CATEGORICAL comparisons were made between groups using chi-squared and Fisher's exact tests. A survival analysis was conducted to determine freedom from AKI between the two groups. Predictor variables were tested by multiple logistic regression. Of the 255 patients included in this study, 110 had ADHF as their primary indication for VA ECLS and 145 patients had other VA ECLS indications. The survival analysis showed that patients with ADHF had a decreased risk of developing AKI on the VA ECLS circuit. Multiple logistic regression revealed no predictors in AKI development between groups and no difference in 30-day mortality was observed.
Conclusion Patients supported by VA ECLS are at high risk of mortality and complications. This research demonstrated that medically complex ADHF patients had less chance of developing AKI when compared with other patients supported by VA ECLS. Future research is needed to investigate potential protective mechanisms of VA ECLS support.
Correlated grammaticalization
Authors: Goldstein David 
Published: June 2022

AbstractGrammaticalization is characterized by robust directional asymmetries (e.g.,Kuteva et al. 2019). For instance, body-part nominals develop into spatial adpositions, minimizers develop into negation markers and subject pronouns become agreement markers. Changes in the opposite direction are either rare or unattested (Garrett 2012: 52). Such robust cross-linguistic asymmetries have led some scholars to reify grammaticalization trajectories as universal mechanistic forces (Heath 1998: 729). One consequence of such a view is that the ambient morphosyntax of a language has little or even no relevance for grammaticalization. This paper uses Bayesian phylogenetic methods to demonstrate the critical role that pre-existing morphosyntax can play in grammaticalization. The empirical basis for this claim is the grammaticalization of definite and indefinite articles in the history of Indo-European: indefinite articles developed at a faster rate among languages in which a definite article had already emerged compared to those lacking a definite article. The two changes are thus correlated. The results of this case study suggest that there is much more to be learned about when and why grammaticalization occurs by investigating its relationship to the pre-existing linguistic system (cf.Reinöhl and Himmelmann 2017: 381).
Primary or secondary wound healing of the pin sites after removal of the external fixator: A monocenter prospective randomized controlled trial
Authors: Roth F , Hoepelman R , van de Wall B , Cagienard F , Babst R , Beeres F 
Published: June 2022

Abstract

Objective
The aim of this monocentre randomized controlled trial was to compare primary wound closure using a single button suture to secondary wound healing of pin sites after removal of the external fixator.

Methods
This non-inferiority trial included all patients who were treated with an external fixator. The primary outcome was infection. Secondary outcomes included all other complications, time to wound healing (in weeks), patients most satisfactory pin site, Visual Analogue Scale (VAS) for pain and the Vancouver Scar Scale (VSS) measured at every 2, 6, 12, 24 and 52 weeks. The most proximal pin site was randomly allocated (1:1) to either primary closure or secondary wound healing, while the following pin sites were treated alternately. Physicians at follow-up were blinded to the closure technique.

Results
A total of 70 patient, providing 241 pin sites were included between 1st January 2019 and 1st March 2020. One-hundred-twenty-three pin sites were treated with primary closure and 118 with secondary wound healing. Median age was 55 (46–67), 44% was male and median duration of the external fixator was 6 days (4–8).There was no significant difference in pin site infections (2% in the primary closure group versus 0% in the secondary healing group). Wound healing was significantly faster in the primary closure group (median 2 vs. 6 weeks, p=0.013). Although not statistically significant, patients seemed more satisfied with the primary closed pin sites (55%). The Vancouver Scar scale showed no differences between groups.

Conclusion
Primary closure of external pin sites does not result in higher infection rate than secondary wound healing and pin sites healed significantly faster after primary closure. Primary closure, therefore, should be the preferred method.
Prognostic factors in patients with acute mesenteric ischemia – a novel tool for determining patient outcomes
Authors: Sinz S , Schneider M A , Graber S D , Alkadhi H , Rickenbacher A , Turina M 
Published: June 2022

Abstract

Objective
Acute mesenteric ischemia (MI) is a devastating disease with poor prognosis. Due to the multitude of underlying factors and broad clinical presentation, prediction of outcomes remains poor. Here, we aimed to identify factors governing survival in MI and develop a novel prognostic tool.

Methods
The present study is a monocentric retrospective analysis of patients with suspected MI undergoing imaging between January 2014 and December 2019. Primary endpoint was identification of factors influencing survival in patients undergoing surgery for confirmed MI, secondary endpoint was the development of a predictive nomogram to aid in determination of patient prognosis.

Results
539 patients underwent CT imaging for suspected MI, 216 of which had radiological indication of MI and 137 of those had subsequently confirmed MI. Factors associated with confirmed ischemia were increasing age, nausea/vomiting, history of peripheral arterial disease and presence of pneumatosis intestinalis and dilated bowel loops.
125 patients underwent surgery, 58 of which survived, and 67 died (median 9 days after diagnosis, IQR: 22). Baseline characteristics, including time from diagnosis to surgery were not different among patients, and outcomes were not affected by the presence of a surgical senior consultant or daytime of the operation. A minimally invasive approach was attempted more often in survivors (25.9% vs. 10.4%, p=0.024), however, in all but 1 patient (1.7%) conversion to open surgery was performed following detection of ischemia.
Increasing age and ASA score, type 2 diabetes, decreased haemoglobin and pH, increased creatinine, radiological atherosclerosis, vascular occlusion (versus non-occlusive MI) and affection of the colon (compared to small bowel ischemia only) were independently associated with impaired survival. Based on a multivariate model, we developed a nomogram for survival prediction, which showed adequate power upon internal validation (C-index = 0.738, Figure 1).

Conclusion
Acute MI remains a condition with high mortality. Here, we identified factors affecting survival after MI, namely colonic involvement, vascular co-morbidities and increasing age. Minimally invasive surgical approaches have not yet gained widespread acceptance in treatment of confirmed MI. Our novel nomogram might prove helpful in outcome prediction of patients with MI.
Attitudes of primary care physicians towards bariatric surgery in Switzerland – a free-word association networks analysis
Authors: Müller V , Gero D , File B , Widmer J , Thalheimer A , Bueter M 
Published: June 2022

Abstract

Objective
Primary care physicians (PCP) have a major responsibility in identifying patients with obesity eligible for bariatric surgery (BS). The aim was to explore PCPs’ attitudes towards BS in order to map potential barriers of referral.

Methods
3526 PCPs in Switzerland were invited to participate in an online survey. PCPs were asked to write the first 5 words coming to their mind about the term “bariatric surgery”. Additionally, they had to pick 2 emotions that best described each provided association. Demographic data and obesity-related referral patterns were collected. Cognitive representation network was constructed based on co-occurrence of associations, using validated data-driven methodology.

Results
216 PCPs completed the study. Respondents were aged 55±9.8 years, had an equal gender distribution and practiced mainly in urban setting (Table 1). The majority of PCPs refer either often (31%) or sometimes (41%) patients for BS, while 7% never refer patients to BS, even if eligibility criteria are met. The PCPs’ overall satisfaction with BS was high (88.5%)(Fig. 1). PCPs could be categorized in 3 subgroups based on their cognitive representation of BS: a) indication-focused (most frequent associations: “obesity”, ”diabetes”/most frequent emotion: “worrying”), b) treatment-focused (“gastric bypass”, “weight loss”/“interested”), and c) risk vs. benefit-focused (“complications”, “challenging follow-up”, “success”/“alert”) (Fig. 2&3). There were no relevant demographic or practice related differences between PCPs in the 3 cognitive modules. PCPs with a treatment-focused mindset had a significantly better knowledge of current guidelines on indications of BS (χ2=17.1, P=0.029) and referred in average 5 patients/year, x2 more than PCPs belonging to the other cognitive modules.

Conclusion
PCPs in Switzerland approve the efficacy of BS, but show reservation to refer eligible patients systematically. PCPs think about BS along 3 cognitive schemes, and the treatment-focused attitude was coupled with the highest willingness to refer eligible patients for BS. Confident knowledge of current indications of BS was the only significant factor that distinguished PCPs with a treatment-focused attitude. Strengthening the collaboration in therapeutic decision-making between PCPs and BS centers has the potential to improve access to optimal care for patients with obesity.