bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Evaluation of Goldeye Life History Characteristics in Two Kansas Reservoirs
Authors: Miller Brett T. , Flores Ernesto , Waters D. Scott , Neely Ben C. 
Published: February 2022

AbstractGoldeye Hiodon alosoides are native through much of Canada and the Mississippi River drainage in the United States. Life history characteristics have been described for several commercially important populations in northern portions of their range, but there is a paucity of information available for more southernly populations. Milford and Lovewell Reservoirs are in the Kansas River basin and harbor the only two lentic Goldeye populations in Kansas. Further, anecdotal observations suggest declining population abundance. We sought to examine temporal trends in relative abundance and describe current size structure, body condition, and population dynamics of these two remnant populations. Both populations had decreasing relative abundance over the previous 24-y period, although decreases in the Lovewell population were most profound. In Milford Reservoir, the median total length of sampled Goldeye was 268 mm (minimum = 235 mm; maximum = 431 mm). The estimated maximum observed age for Goldeye was 8; however, age-1 Goldeye were the most abundant year-class that we collected, accounting for 80% of sampled fish. We fit a von Bertalanffy growth model to back-calculated length at annulus formation with parameter estimates of L∞ = 402 mm, K = 0.93, and t0 = 0.29. We collected two individuals at Lovewell Reservoir; therefore, no analyses were conducted. Overall, this study provides cursory knowledge of two remnant Goldeye populations in Kansas. These results can be applied to promote better understanding of their ecological role in these reservoirs and other midwestern impoundments with remnant populations.
Short Communication: Case Study on Stock Verification in a Semi-automated Environment with Special Reference to the Library of the Open University of Sri Lanka
Authors: Marasinghe M. M. I. K. , Balasooriya A. H. K. , Amarasekara K. M. R. K. , Abeysekera K. H. T. , Gunasekara R. M. , Wickramarathne K. D. 
Published: February 2022

Stock verification is considered as an essential activity for all types of libraries although the libraries face difficulties in managing it with the regular library works. Development of a simple method to carry out the stock verification of less technologically oriented libraries would benefit the libraries in numerous ways. Therefore, the objectives of the present study were to document the stock verification process carried out by the library of the Open University of Sri Lanka and to elaborate on the outcomes and challenges of the stock verification procedure carried out by the library of the Open University of Sri Lanka. This will serve as a reference document to libraries that functioned under a semi-automated environment. The study details the procedure followed by the Open Universitylibrary while employing a minimum of library resources such as library staff, capital, management, etc. The whole process was conducted in 02 phases as;01) physical verification, which involved extracting required data from each book in the physical collection and identifying obsolete, outdated books and books to be repaired and02) data comparison with the accession register to identify lost/ missing books. The study also details the outcomes of the processwhich include deriving a simple method with minimal human and mechanical errors which enables periodically carrying out the process, increasing the quality and the currency of the book collection,enhancing the library staff skills, and the overcoming the challenge of managing the process with minimum staff, who are parallelly carrying out their routine work at the library.
Evaluating acephate and azadirachtin for control of Psyllopsis discrepans (Flor) (Hemiptera: Psyllidae) and prevention of decline of ash trees
Authors: Boone Jeff , Wist Tyler J. , Prager Sean M. 
Published: February 2022

Abstract
Over the past 20 years, ash trees (Oleaceae) in parts of the western United States of America and Canada have been subject to infestations with the psyllid Psyllopsis discrepans (Flor) (Hemiptera: Psyllidae). Infested trees show a series of symptoms, including pseudogalls, canopy loss, and in many cases, tree death. This is an expensive problem for urban forests, particularly in the context of emerald ash borer (Coleoptera: Buprestidae) and Dutch elm disease (Ophiostomataceae), which also impact the diversity of urban forests. This paper presents results from a study on the efficacy of two tree-injected insecticides, Orthene® (acephate) and TreeAzin® (azadirachtin). Trees were treated with these insecticides, and egg and adult psyllids were counted. In addition, canopy cover and severity of pseudogalling were visually assessed. Orthene reduced canopy loss and severity and amount of pseudogalling compared to what occurred on control trees; however, there were more eggs on Orthene-treated trees, indicating that any potential benefit was offset by higher egg counts after treatment. Due to the rapid decline of the ash trees, TreeAzin could not be successfully injected.
ATF7ip Targets Transposable Elements for H3K9me3 Deposition to Modify CD8+ T Cell Effector and Memory Responses
Authors: Sin Jun Hyung , Kashyap Sujit , Acenas Dante , Cortez Jessica T , Lee James , Marson Alexander , Matloubian Mehrdad , Waterfield Michael R 
Published: February 2022

Abstract
CD8+ T cells are critical for the immune response to pathogens and tumors, and CD8+ T cell memory protects against repeat infections. In this study, we identify the activating transcription factor 7 interacting protein (ATF7ip) as a critical regulator of CD8+ T cell immune responses. Mice with a T cell–specific deletion of ATF7ip have a CD8+ T cell–intrinsic enhancement of Il7r expression and Il2 expression leading to enhanced effector and memory responses. Chromatin immunoprecipitation sequencing studies identified ATF7ip as a repressor of Il7r and Il2 gene expression through the deposition of the repressive histone mark H3K9me3 at the Il7r gene and Il2–Il21 intergenic region. Interestingly, ATF7ip targeted transposable elements for H3K9me3 deposition at both the IL7r locus and the Il2–Il21 intergenic region, indicating that ATF7ip silencing of transposable elements is important for regulating CD8+ T cell function. These results demonstrate a new epigenetic pathway by which IL-7R and IL-2 production are constrained in CD8+ T cells, and this may open up new avenues for modulating their production.
Group B Streptococcus Surface Protein β: Structural Characterization of a Complement Factor H–Binding Motif and Its Contribution to Immune Evasion
Authors: Xu Xin , Lewis Marffy Alexander L , Keightley Andrew , McCarthy Alex J , Geisbrecht Brian V 
Published: February 2022

Abstract
The β protein from group B Streptococcus (GBS) is a ∼132-kDa, cell-surface exposed molecule that binds to multiple host-derived ligands, including complement factor H (FH). Many details regarding this interaction and its significance to immune evasion by GBS remain unclear. In this study, we identified a three-helix bundle domain within the C-terminal half of the B75KN region of β as the major FH-binding determinant and determined its crystal structure at 2.5 Å resolution. Analysis of this structure suggested a role in FH binding for a loop region connecting helices α1 and α2, which we confirmed by mutagenesis and direct binding studies. Using a combination of protein cross-linking and mass spectrometry, we observed that B75KN bound to complement control protein (CCP)3 and CCP4 domains of FH. Although this binding site lies within a complement regulatory region of FH, we determined that FH bound by β retained its decay acceleration and cofactor activities. Heterologous expression of β by Lactococcus lactis resulted in recruitment of FH to the bacterial surface and a significant reduction of C3b deposition following exposure to human serum. Surprisingly, we found that FH binding by β was not required for bacterial resistance to phagocytosis by neutrophils or killing of bacteria by whole human blood. However, loss of the B75KN region significantly diminished bacterial survival in both assays. Although our results show that FH recruited to the bacterial surface through a high-affinity interaction maintains key complement-regulatory functions, they raise questions about the importance of FH binding to immune evasion by GBS as a whole.
Learning Curve of Thoracic Pedicle Screw Fixation by Freehand Technique
Authors: Abbas Zahir , Asati Sanjeev , Kundnani Vishal G. , Jain Sanyam , Patel Ankit , Raut Saijyot 
Published: February 2022

Background:
The freehand method of inserting thoracic pedicle screw has become one of the most popular techniques; however, data on its learning curve are scanty. The purpose of this study was to delineate the learning curve and to evaluate the safety of freehand technique of thoracic pedicle screw placement in nondeformed spine.

Materials and Methods:
A total of 92 consecutive patients who underwent thoracic posterior stabilization with pedicle screws using freehand technique between 2012 and 2017 in various pathologies of nondeformed spine from T1 to T10 at a single institution by a single surgeon were analyzed. Patients were divided into four quartiles (Q1, Q2, Q3, Q4, with 23 patients each) with each consecutive group serving as control for its prior. Demographics (age, sex, pathology involved) and complications were evaluated. Postoperative computed tomography (CT) scan was taken for evaluation of screws perforation including level, direction, grade, and severity of perforation.

Results:
Of total of 735 screws inserted in 92 patients, 72 screws were perforated with a perforation rate of 9.79%. Of the total perforations, more than half (63.88%) were of Grade 2 and maximum perforations were seen in the lateral direction (58.3%). Total three critical perforations were noted but none of them were symptomatic. The highest rate of perforation was evident at T4 vertebra (18.29%), whereas it was lowest at T9 (3.79%). The perforation rate showed a statistically significant (P < 0.05) decline in Q2 as compared to Q1 achieving asymptote in Q1 after approximately 80–100 screws.

Conclusion:
A steep learning curve is associated with the freehand technique of thoracic pedicle screws and asymptote can be achieved after approximately 80–100 screws. Novice surgeons can reduce the learning curve by doing practice on saw bone models and cadaveric dissection learning to avoid perforations and other complications with understanding the complex anatomy and variations encountered in the typical thoracic spine.
High faecal carriage of extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) among hospitalized patients at Sylvanus Olympio Teaching Hospital, Lomé, Togo in 2019
Authors: Godonou A.M. , Lack F. , Gbeasor-Komlanvi F.A. , Konlani L. , Dossim S. , Ameyapoh Y.A. , Ekouevi K. , Dagnra A.Y. , Salou M. 
Published: February 2022

Background: Extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) are a global health concern, associated with increased morbidity and mortality. Even in the absence of infections, colonization by these pathogens is still a great threat because of the risk of cross transfer among hospitalized patients. Faecal carriage of ESBL-PE remained poorly documented in Africa. This study aimed to determine faecal carriage rate of ESBL-PE, factors associated with carriage, and antimicrobial susceptibility of the strains among hospitalized patients at Sylvanus Olympio Teaching Hospitals (CHU SO) in Lomé, Togo.
Methodology: This was a cross-sectional study of 105 randomly selected hospitalized patients between September and November 2019. Socio-demographic and clinical data as well as rectal swabs were collected after obtaining the consent of the selected participants. Rectal swabs were cultured on selective bromocresol purple (BCP) lactose agar containing 6µg/l ceftazidime, for isolation of Enterobacteriaceae. Identification of each isolate was performed using Uriselect 4 medium and API 20E. Antibiotic susceptibility of the bacterial isolates was performed by the Bauer-Kirby agar disc diffusion test and interpreted according to CASFM-EUCAST recommendations.
Results: The faecal carriage rate of ESBL-PE among selected hospitalized patients was 80.9% (85/105). Escherichia coli was the most frequent bacteria 69.5% (73/105), followed by Klebsiella pneumoniae 22.8% (24/105). The antibiotic profile of ESBL producing Escherichia coli showed resistance to amoxycillin+clavulanic acid (72.6%), ticarcillin+clavulanic acid (82.2%), piperacillin+tazobactam (30.1%), cefoxitin (30.1%) ciprofloxacin (84.9%), levofloxacin (76.7%), nalidixic acid (83.6%), chloramphenicol (26.0%), gentamicin (49.3%), sulfamethoxazole-trimethoprim (86.3%), imipenem (5.5%), and ertapenem (21.9%). All (100%) isolates were sensitive to amikacin and fosfomycin. None of the characteristics or risk factors assessed was significantly associated with faecal carriage of ESBL-PE.
Conclusion: Faecal carriage rate of ESBL-PE in these hospitalized patients was very high, but no factor was associated with carriage of ESBL-PE among the study population. Implementation of infection control measures, and surveillance are needed to limit the spread of these resistant pathogens within CHU SO healthcare facilities.
Formulation, characterization and in-vitro evaluation of solid lipid nanoparticles for the delivery of a new anticancer agent, 1H-pyrazolo[3,4-d] pyrimidine derivative
Authors: Elsayed Amani , Belal Amany 
Published: February 2022

Purpose: To investigate the physicochemical properties and in vitro cytotoxic effect of a potent epidermal growth factor receptor-tyrosine kinase (EGFRWT-TK) inhibitor, 1H-pyrazolo [3,4-d] pyrimidine (FEP) derivative and formulated as solid lipid nanoparticles (SLNs) using stearic acid (ST) or glycerylmonostearate (GMS).
Methods: The SLNs were prepared by hot homogenization and sonication method. The effect of formulation variables on particle size, zeta potential and polydispersity index (PDI) of SLNs were studied, and an optimized formulation selected. Drug-excipient interactions were assessed by differential scanning calorimetry (DSC) and Fourier Transform Infrared (FTIR). Mammary gland breast cancer (MCF-7) and human colon cancer (HCT116) human cell lines were used to evaluate the cytotoxic activity of the free and FEP-loaded SLNs.
Results: The particle size of the SLNs was in the range of 138 - 819 nm, while zeta potential varied from -15 to -20 nm. FEP-loaded SLNs exhibited significant cytotoxic effect compared to the free drug and doxorubicin in the two cell lines (p < 0.05). The activity was higher in HCT116 compared with MCF-7 cells (p < 0.007). The concentration of FEP loaded SLNs, free drug and doxorubicin that showed 50 % inhibition (IC50) for breast cancer cells were 1.06 ± 0.09, 2.58 ± 0.16 and 3.75 ± 0.4 μg/ml, respectively.
Conclusion: The findings show that FEP-loaded SLNs have greater in-vitro cytotoxic activity than the free FEP, and thus, might improve cancer therapy in humans.
Anxiety and depression among pharmacy students before and during COVID-19 pandemic
Authors: Al-Qerem Walid , Hammad Alaa , Amawi Haneen A. , Jarab Anan S. , Ling Jonathan 
Published: February 2022

Purpose: To evaluate factors associated with anxiety and depression among pharmacy students before and during COVID-19 pandemic.
Methods: The Beck Depression Inventory-II (BDI-II) and the State-Trait Anxiety Inventory (STAI) questionnaires were translated into Arabic and placed on the e-learning platforms of two Jordanian universities after conducting cognitive interviews. Validity and reliability of the two translated questionnaires were evaluated by conducting Exploratory Factor Analysis (EFA) and calculating Cronbach’s alpha. T-tests and binary regression were conducted to evaluate factors associated with anxiety and depression among pharmacy students.
Results: Several factors were associated with depression and anxiety including being an international student (p < 0.05), living alone and away from their primary country of residence (p < 0.05), female gender (p < 0.01), and being a fifth-year student (p < 0.01).
Conclusion: A tool to evaluate anxiety and depression among pharmacy students in Jordan has been successfully validated. Also, the results show that pharmacy students suffered from high levels of anxiety and depression, particularly during the COVID-19 pandemic.
LncRNA-NEAT1/miR-148a-3p axis regulates cell viability, apoptosis and autophagy through wnt/β-catenin signaling pathway in Breast Cancer
Authors: An Song , Xia Yuren , Gao Zilu , Sun Xiaoxuan , Wang Jian 
Published: February 2022

Purpose: Breast cancer has over the years been one of major acute carcinomas in women. This study investigated the fundamental mechanistic functions of the lncRNA-NEAT1/miR-148a-3p/Wnt/β-catenin axis in moderating cell viability, apoptosis and autophagy in Breast Cancer (BC).
Methods: RT-qPCR measured expression of lncRNA NEAT1 and microRNA-148a-3p in human cell lines for Breast Cancer. Cell transfection upregulated or silenced the genes with CCK-8, western blot and FCM apoptosis assays determining the cellular growth, proliferation and protein expression related to autophagy biomarkers. Furthermore, luciferase assay analyzed the luciferase activity of lncRNA- NEAT1 and microRNA-148a-3p
Results: The outcomes indicated that LncRNA-NEAT1 was upregulated in BC cell lines and promoted cell viability, autophagy and inhibited Apoptosis in BC cells. However, lncRNA-NEAT1 knockdown inhibited cell viability, autophagy and enhanced apoptosis. In addition, lncRNA-NEAT1 directly targeted microRNA-148a-3p. And, it was found that microRNA-148a-3p overturns the cellular viability, autophagy and inhibitory effects on Apoptosis imposed by lncRNA-NEAT1 overexpression. Lastly, overexpressed lncRNA-NEAT1 activated the Wnt/β-catenin regulatory network through sponging microRNA-148a-3p in BC cell lines.
Conclusion: The present study showcased that lncRNA-NEAT1 could enhance tumor development in breast cancer via playing the role of molecular sponge to microRNA-148a-3p, and eventually hyper invigorating the Wnt/β-catenin regulatory network.
MiR-875-5p suppresses cervical cancer cell proliferation and metastasis via negative regulation of EGFR
Authors: Liang Hua , Zhao Yuzi , Pi Jie , Luo Ruoyu 
Published: February 2022

Purpose: To explore miRNA-875-5p and epidermal growth factor receptor (EGFR) activities in tissues or cells from cervical cancer, and their underlying molecular mechanisms.
Methods: Tissues were obtained from cervical cancer patients and their miR-875-5p expression was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Caski or HeLa cells were transfected with miR-875-5p mimics or miR-875-5p inhibitor to assess the effect of miR-875- 5p expression on cell viability, cell cycle, migration, and invasion using Cell Counting Kit-8 (CCK-8), flow cytometry, wound healing, and Transwell assays. Potential target genes of miR-875-5p were predicted and verified using a dual luciferase reporter assay. In addition, EGFR expression was evaluated by western blot.
Results: MicroRNA-875-5p was expressed at low levels in cervical cancer tissues and was related to FIGO stage, lymph node metastasis, pathological grade, vascular involvement, and deep stromal invasion in patients with cervical cancer. MicroRNA-875-5p overexpression inhibited cell viability, migration, and invasion, and caused G0/G1 phase block of Caski and HeLa cells. Moreover, EGFR was the target gene of miR-875-5p and was negatively regulated by miR-875-5p. Reductions in cell viability, migration, invasion, and the number of G0/G1-phase cells were inhibited by EGFR overexpression.
Conclusion: MiR-875-5p suppresses cervical cancer cell growth and metastasis by negatively regulating EGFR. Therefore, miR-875-5p can potentially be exploited for the management of cervical cancer.
Faktor Pengaruh Penerimaan Opini Audit Going Concern
Authors: Kristianasari Eka Putri , Ismawati Kun 
Published: February 2022

The going concern audit opinion given by an independent auditor to a company shows the auditor’s doubt about the business continuity of the company so that it become a reference for investors and creditors in making decisions. This study aims to analays and provide empirical evidence regarding the effect of company’s growth, company’s size, and prior opinioin to going concern audit opinion.The population in the study were manufacture companies listed on the Indonesia Stock Exchange (BEI) for the period 2017-2019 totaling 175 companies. Using purposive sampling in determining samples that 105 manufacturing companies were selected with a research periode of 3 years, then the number of units the research samples is 105 x 3 = 315 data. Data collection techiques used are documentation techniques and the data analys technique used in this study was logistic regression analys using SPSS windows software version 25. Statistical analys showed that the company’s growth did not affect on the acceptance of going concern audit opinion. While company’s size and the prior opinion significantly influenced the acceptance of going concern audit opinion.
ABSTRAK
Opini audit going concern yang diberikan oleh auditor independen kepada sebuah perusahaan menunjukkan adanya keraguan auditor terhadap keberlangsungan usaha perusahaan, sehingga menjadi bahan acuan bagi investor ataupun kreditor dalam mengambil keputusan. Penelitian ini bertujuan untuk menganalisis dan memberikan bukti empiris mengenai pertumbuhan perusahaan, ukuran perusahaan, dan opini audit tahun sebelumnya terhadap penerimaan opini audit going concern.Populasi dalam penelitian ini adalah perusahaan manufaktur yang terdaftar di BEI periode 2017-2019 sebanyak 175 perusahaan. Menggunakan teknik purposive sampling dalam menentukan sampel terpilih 105 perusahaan manufaktur dengan masa penelitian selama 3 tahun. Jumlah sampel penelitian adalah 105 x 3= 315. Teknik pengumpulan data yang digunakan ialah teknik dokumentasi dan teknik analisis data yang digunakan adalah regresi logistik menggunakan software SPSS versi 25. Hasil analisis statistik menunjukkan bahwa pertumbuhan perusahaan tidak berpengaruh terhadap opini audit going concern. Sedangkan ukuran perusahaan dan opini audit tahun sebelumnya berpengaruh signifikan terhadap penerimaan opini audit going concern.