bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of katG and inhA Gene Mutation in Rifampicin-resistant Mycobacterium Tuberculosis Using Line Probe Assay (LPA) in Kebbi, Nigeria.
Authors: Manga Shuaibu Bala , Danladi Yusuf Kanya , Olaosebikan Victor Oluwatosin , Adedokun Ayodele Isaac , Udefi Augustine Chijioke 
Published: September 2022

Abstract

Elimination of tuberculosis remains a public health menace, due to multidrug resistant strains of Mycobacterium tuberculosis, that are resistant to the first-line anti-tubercular drugs. Consequently, detection of resistant strains depends on early diagnosis. Intervention of molecular techniques is essential to reduce mortality and morbidity rates. This study aimed to detect katG and inhA gene mutations among rifampicin resistant strain of Mycobacterium tuberculosis in Kebbi State. A cross-sectional study was conducted which covered all the five major health zones in Kebbi State. Two hundred and forty (240) acid fast bacilli confirmed sputum samples were recruited and subjected to Gene Xpert testing. Positive sputum samples were later subjected to Line Probe Assay technique. 14 sputum samples were resistant to rifampicin out of the 240 confirmed acid fast bacilli sputum samples while 226 were sensitive to rifampicin. Mutations were found at the frequency of 100% for katG gene (katG/S315T) in all the 14 rifampicin resistant samples (rpoB/S531L) at the different health zones in kebbi state using the line probe assay. The research has also detected inhA gene mutation at a frequency of 100% in all rifampicin resistant samples showing that mutation is associated with multi resistance to isoniazid and rifampicin. This study confirms that resistance due to katG and inhA mutation is a better surrogate of multidrug-resistant tuberculosis among TB patients in Kebbi State compared to the single-marker analysis. Hence, early molecular detection of rifampicin resistant strains will suffice in determining the management of multidrug resistant tuberculosis in Kebbi State.
Prognostic Value of NT-pro BNP in Extracorporeal Membrane Oxygenation Assisted Cardiogenic Shock Patients: A 5-Year Single-Center Retrospective Analysis
Authors: Shang Jin , Liu Miaomiao , Yan Yang , Zhang Ying , Guo Fengwei , Song Yan 
Published: September 2022

Abstract
Objective This study aimed to evaluate the predictive value of N-terminal pro-brain natriuretic peptide (NT-pro BNP) for venoarterial extracorporeal membrane oxygenation (VA-ECMO)-assisted clinical outcomes in adult patients with cardiogenic shock (CS).
Methods: Seventy-seven CS patients who underwent VA-ECMO-assisted therapy at our center between January 2016 and January 2021 were included, and their demographic information and clinical data were collected. The prognostic value of NT-pro BNP in these patients was assessed.
Results: Among the study participants, the highest NT-pro BNP values after VA-ECMO assistance were observed in CS patients who had died versus those undergoing rehabilitation (21,439.62 vs. 13,568.26 pg/mL). Mean NT-pro BNP values at the time of ECMO weaning (18,170.95 vs. 8472.8 pg/mL) and before discharge (22,183.35 vs. 5646.197 pg/mL) were higher in the death group. Age; Sepsis-related Organ Failure Assessment scores; creatinine, platelet, blood urea nitrogen, total bilirubin, and lactic acid levels; mean arterial pressure; creatinine level at the time of ECMO weaning; NT-pro BNP value before discharge; and left ventricular ejection fraction percentage were reliable predictors of mortality. The area under the receiver operating characteristic curve (AUC) was >0.70 (P<0.05). The AUC of pre-discharge NT-pro BNP was 0.873, and these NT-pro BNP values had the best predictive ability regarding patient death.
Conclusion:Among CS patients who received VA-ECMO assistance, NT-pro BNP values at each assistance point had important patient-related diagnostic and predictive values. Pre-discharge NT-pro BNP values were the best predictors of patient prognosis.
Postpartum Depressive Symptoms, Perceived Social Support and Their Associations of Women Under Different COVID-19 Pandemic Severities: Analysis of A Chinese Nationwide Multicenter Study
Authors: Li Hailin , Huang Junjie , Chen Haitian , Wang Zilian , Cao Yinli , Qi Hongbo , Ma Yuyan , Bai Xiaoxia , Zhao Yangyu , Liu Caixia , Wei Jun , Wang Hong , Jin Yan , Cai Shiqin , Zhu Yanna 
Published: September 2022

Abstract
Background
The evidence for associations of mental health with COVID-19 pandemic severity is scarce in postpartum women. We aimed to investigate the associations of postpartum depressive symptoms (PDS), perceived social support (PSS) with COVID-19 pandemic severity, respectively, and to explore the associations between PSS and PDS under different pandemic severities.
Methods
This was a nationwide multicenter study. An online survey was launched from January 23 through February 29, 2020 in mainland China. Participants’ PDS and PSS were measured by Edinburgh Postnatal Depression Scale and Multidimensional Scale of Perceived Social Support, respectively.
Results
The final sample consisted of 452 participants from 19 hospitals in 19 provinces/municipalities of mainland China. After covariate adjustment, women in Hubei Province experienced higher PDS levels than those in moderate (β coefficient = − 3.883, p = 0.001) and high (β coefficient = − 3.760, p = 0.005) pandemic severity; women in moderate pandemic severity reported higher PSS levels not only than those in high (β coefficient = − 4.947, p = 0.008) pandemic severity and Hubei Province (β coefficient = − 6.480, p = 0.049), but also than low (β coefficient = − 7.428, p = 0.005) pandemic severity. Also, no significant associations were found between PSS and pandemic severity for women with moderate-to-severe PDS (p > 0.05). There were significantly negative associations between PSS and PDS for women under low (OR = 0.837, p = 0.039), moderate (OR = 0.919, p < 0.001), and high (OR = 0.951, p = 0.012) pandemic severity, and no significant association between PSS and PDS was observed in Hubei Province after covariate adjustment (p > 0.05).
Conclusion
Women in Hubei Province experienced higher PDS levels than those in moderate and high pandemic severity. Women in moderate pandemic severity reported higher PSS levels not only than those in high pandemic severity and Hubei Province, but also than low pandemic severity, and no associations were found between PSS and pandemic severity for women with moderate-to-severe PDS. There were negative associations between PSS and PDS for women in low, moderate and high pandemic severity, but no association between PSS and PDS of women in Hubei Province.
Fuel bed variation in coastal tropical freshwater forested wetlands in three disturbance regimes at La Encrucijada Biosphere Reserve, Mexico
Authors: Barrios-Calderón Romeo de Jesús , Mata Dulce Infante , Garnica José Germán Flores , Torres Jony R. 
Published: September 2022

Abstract
Tropical freshwater forested in coastal regions are rapidly disappearing as a result of wildfires caused by high accumulations of forest fuels, which can vary in quantity and availability. Therefore, the objective of this study was to characterize fuel beds in tropical freshwater forested wetlands with three levels of disturbance at El Castaño, La Encrucijada Biosphere Reserve. Seventeen sampling units were used to describe the structure of the forest’s fuel beds (canopy, sub-canopy, understory). Fallen woody material, surface litter, and fermented litter were characterized using the planar intersection technique. Diversity was composed of eight species of trees, two shrubs, five lianas, and two herbaceous species. The vertical strata were dominated by trees between 2 to 22 m high. The horizontal structure had a higher percentage of trees with a diameter at breast height between 2.5 and 7.5 cm (61.4%) of the total. The non-disturbed sites presented the highest arboreal density (2686 ind. ha −1 ). The richness and diversity of species showed that the Fisher, Margalef, Shannon, and Simpson α indices were higher in the undisturbed sites. The Berger-Parker index exhibited greater dominance in the site’s with high disturbance. Pachira aquatica showed the highest importance value index and was the largest contributor to fuel beds. The sites with the greatest disturbance had a greater accumulation of dead fuel beds, with the greatest accumulations of woody fuels in classes 1, 10, and 1000 h (rotten). This study contributes to defining the areas prone to fire in these ecosystems and designing prevention strategies.
Retinol-binding protein type 1 expression predicts poor prognosis in head and neck squamous cell carcinoma
Authors: Fu Ling-ling , Yan Ming , Zhang Yi , Yu Xin , Shao Min , Gosau Martin , Friedrich Reinhard E. , Vollkommer Tobias , Smeets Ralf , Hu Xiang-zhi , Feng Hong-chao , Xu Li-ya 
Published: September 2022

Abstract

Background
Head and neck squamous cell carcinoma (HNSCC) is the sixth most prevalent malignancy worldwide, with high incidence and poor survival rates. RBP1 is highly expressed in several kinds of cancer and plays a potential prognostic factor. However, the relationship between RBP1 and HNSCC were analyzed based on The Cancer Genome Atlas (TCGA) database.
Materials and Methods
RBP1 expression and clinical information were obtained from the Cancer Genome Atlas (TCGA) database. Tumor tissue and adjacent normal tissue of 3 HNSCC patients were collected to analyze the RBP1 mRNA expression level by quantitative PCR. Cox regression analysis was used to evaluate the prognostic values of RBP1 and clinical data in HNSCC. A nomogram was also established to predict the impact of RBP1 on prognosis based on Cox multivariate results. The methylation level of RBP1 in HNSC and its prognosis were analyzed in UALACN and MethSurv. Finally, the potential biological functions of RBP1 were investigated using gene set enrichment analysis (GSEA) and single sample GSEA (ssGSEA).
Results
The mRNA expression level of RBP1 was highly expressed in HNSCC tissue. The multivariate analysis result indicated that RBP1 is an independent prognosic factor in HNSCC(P < 0.05). ROC curve analysis showed that RBP1 had good presictive accuracy for prognosis (area under the ROC curve: 0.887, sensitivity: 84.1%, specificity: 79.9%). The methylation level of RBP1 in HNSCC tissues was higher than that in adjacent non-tumor tissues, six CpG sites were found associated with better prognosis. Additionally, RBP1 expression is mildly associated with immune cell infiltration and immunological checkpoints.
Conclusion
RBP1 is overexpressed and associated with poor patient prognosis in head and neck squamous cell carcinoma.
Critical role of VHL/BICD2/STAT1 axis in crystal-associated kidney disease
Authors: Lu Dan , Hao Wenyan , Hong Peng , Zhang Xin , Zhao Xuyang , Ma Lulin , Ping Hao 
Published: September 2022

Abstract
Nephrolithiasis is highly prevalent and associated with the increased risk of kidney cancer. The tumor suppressor von Hippel-Lindau (VHL) is critical for renal cancer development, however, its role in kidney stone disease has not been fully elucidated until now. Here we reported VHL expression was upregulated in renal epithelial cells upon exposure to crystal. Utilizing Vhl+/mu mouse model, depletion of VHL exhibited severe kidney injury during nephrolithiasis. Conversely, overexpression of VHL limited crystal-induced lipid peroxidation and ferroptosis in a BICD2-depdendent manner. Mechanistically, VHL interacted with the cargo adaptor BICD2 and promoted its K48-linked poly-ubiquitination, consequently resulting in the proteasomal degradation of BICD2. Through promoting STAT1 nuclear translocation, BICD2 facilitated IFNγ signaling transduction and enhanced IFNγ-mediated suppression of cystine/glutamate antiporter system Xc−, eventually increasing cell sensitivity to ferroptosis. Moreover, we found that the BRAF inhibitor impaired the association of VHL with BICD2 through triggering BICD2 phosphorylation, ultimately causing severe ferroptosis and nephrotoxicity. Collectively, our results uncover the important role of VHL/BICD2/STAT1 axis in crystal kidney injury and provide a potential therapeutic target for treatment and prevention of renal inflammation and tumorigenesis.
The Impact of Psychological Resilience on Chronic Patients’ Depression During the dynamic Zero-COVID policy: The Mediating Effect of Stigma and the Moderating Role of Sleep
Authors: Mei Yujin , Yang Xue , Liu Changjun , Li Yuqing , Gui Jiaofeng , Zhang Lin 
Published: September 2022

Abstract

Objective
Chronic patients are experiencing depression caused by themselves or the surrounding environment, how to cope with the change of mentality and adjust the psychological stress response, especially under the background of the current dynamic Zero-COVID policy in China, is a problem worth further discussion. The researchers constructed a mediating regulation model to test the influence of psychological resilience on depression of chronic patients during dynamic Zero-COVID, as well as the mediating role of stigma and the regulating role of and the moderating role sleep.
Method
From October 2021 to February 2022, a multi-stage stratified sampling method was used to collect data in Shangcheng District of Hangzhou City, Zhejiang Province. We used the random number table method. Firstly, a third-class hospital was randomly selected from Shangcheng District. Secondly, departments were randomly selected from the hospital. Thirdly, survey points were set up in each department, and chronic patients were randomly selected from each survey point. In addition, chronic medical illness burden was assessed using the Cumulative Illness Rating Scale-Geriatrics (CIRS-G), psychological resilience was measured by the Conner-Davidson Resilience Scale (CD-RISC), stigma was measured by the Stigma Scale for Chronic Illness (SSCI), sleep was measured by the Pittsburgh Sleep Quality Index(PSQI) and depressive symptoms were estimated by the Patient Health Questionaire-9(PHQ-9). SPSS (version 21) and PROCESS (version 4.0) were used for correlation analysis, mediation analysis, and mediated moderation analysis.
Results
Psychological resilience was negatively correlated with depression, stigma and sleep. Depression was positively correlated with stigma and sleep. Stigma and sleep were positively correlated; Stigma played a mediating role in the relationship between psychological resilience and depression; Sleep moderated the first half of the pathway " psychological resilience → stigma → depression" .
Conclusion
Psychological resilience affected depression directly and also indirectly through stigma. At the same time, sleep played a moderating role between psychological resilience and depression. The correlation between psychological resilience and stigma was stronger when levels of sleep levels were higher.
Andersen’s behaviour model on determining the factors associated with maternal healthcare services utilisation in Papua New Guinea
Authors: Li Hao , Pu Yiran , Li Zhen , Jin Ziyang , Jiang Yi 
Published: September 2022

Abstract
Background: Papua New Guinea (PNG) has recorded the highest maternal mortality ratio in the Western Pacific Region and faces major challenges in achieving SDG 3. Antenatal care (ANC), skilled birth attendant (SBA) and postnatal care (PNC) services are critical components of maternal healthcare services (MHS) for reducing maternal mortality and promoting maternal health in PNG. The study sought to assess the prevalence and determinants of ANC, SBA and PNC services amongst women in PNG.
Methods: The study was conducted using the 2016–2018 Papua New Guinea Demographic and Health Survey. A total of 5248 reproductive-age women were considered as the analytical sample. The outcome variables were utilisation of ANC, SBA and PNC services. Chi-square test, multivariable logistic regression and dominance analysis were conducted. Statistical significance was set at p<0.05.
Results: The prevalence rates of ANC, SBA and PNC services were 52.3%, 58.7% and 26.6%, respectively. Women’s employment, education, media exposure, distance to health facility, household wealth, region, residence and parity were determinants of MHS utilisation. ANC, SBA and PNC services utilisation were all primarily influenced by enabling factors, followed by predisposing and need factors.
Conclusions: This study demonstrated that enabling factors such as media exposure, distance to health facility, household wealth, region and residence have the greatest impact on MHS utilisation, followed by predisposing (working, education) and need factors (parity). Therefore, enabling factors should be prioritised when developing maternal health programmes and policies. For example, transport and health infrastructure should be strengthened and women’s education and vocational training should be increased, especially in Highlands region, Momase region and rural areas, to increase the utilisation of MHS.
Construction and Application of Frailty Assessment and Early Warning Platform for the Community Elderly: Based on a Cross-sectional Survey
Authors: Li Jing , Peng Hua , Huang Jia , Yue Liqin , Gao Wuqiang , Liu Guanyu , Zeng Shumei , Li Fang , Huang Weihong , Hu Jianzhong 
Published: September 2022

Abstract
Background: Health management for elderly in the community is more difficult, especially under the impact of the COVID-19 pandemic. This study aims to build a frailty assessment platform and form a frailty early warning model by Machine Learning (ML) methods that community health workers can use to carry out overall evaluation and health management of the elderly.
Methods:The intra-group correlation coefficient (ICC) was calculated to test the platform's reliability and conduct a user satisfaction survey. The frailty assessment results were taken as the dependent variable, and the comprehensive assessment results, the elderly capability assessment results, and the basic information of the elderly were taken as independent variables to train and develop a frailty risk model by ML methods. The model performance was evaluated by Precision, Recall, F1-score, Accuracy, area under the ROC curve (AUC value), Macro avg, Weighted avg and other indicators. The visual image SHapley Additive exPlanations (SHAP) method was used to analyze the features of the effective model, the risk factors, and the population distribution of frailty, thus forming a frailty early warning model.
Results: The reliability test of frailty assessment and early warning platform showed high consistency with ICC of 0.966, and a 95% confidence interval (95% CI) of (0.888, 0.990), P<0.001. The user satisfaction survey showed the highest score for necessity. The cross-sectional survey showed that the frailty rate of the elderly reached 34.5%,and fitting support vector machine with RBF kernel function (SVM RBF) had the best performance in ML methods with AUC=0.862 in the Training set and AUC=0.865 in the Test set. The SHAP analysis showed that positive sarcopenia, high risk of falls, potential and above anxiety, and moderate and above impairment of daily living ability were more likely to cause frailty. The elderly with all four features accounted for 11.56% of the entire elderly population.
Conclusions: The frailty assessment and early warning platform constructed in this study have good reliability and high user satisfaction. By running the SVM RBF model in the platform background thread, the platform named “I can help”, was developed for community elderly and health workers.
DNA barcoding and nutritional profiling of some underutilized native indigenous plant species (NIPS) of Karnataka, India
Authors: KUMAR ROSHNI MOHAN , R Bharathi T , S Prakash H 
Published: September 2022

Abstract
Locally adapted native indigenous plant species (NIPS) could restore the crop diversity in sustainable agriculture. Here, we report the molecular identification and nutritional profiling of some five NIPS of Karnataka; Musa paradisiaca cv. Nanjangud rasabale, Piper betle L. cv. Mysore betel leaf, Jasminum grandiflorum cv Mysore mallige, Solanum melongena L. cv. Udupi Mattu Gulla and S. melongena L. cv. Erangere badane of which the first four are Geographical Indication (GI) tagged. The samples were procured, authenticated and sequenced using two standard DNA barcodes; nuclear ITS2 and plastid rbcl. The phylogenetic analysis using Neighborhood joining method revealed all the ITS2 tree topologies with higher genetic divergence than rbcl. All the rbcl tree topologies were monophyletic indicating sequence conservation. Though the concatenated ITS2 + rbcl trees had higher bootstrap support (> 98% except Solanum sp.) differences were observed because of the lack of available sequence deposition at species level. The proximate and nutritional profiling of the NIPS displayed superiority in terms of their nutritional profile and their potential application in phytopharmaceutical sector as nutritional supplements. We anticipate that if research towards the identification of NIPS species is accelerated, these nutritionally enhanced crops could be used as a safe and sustainable food in changing global climatic conditions.
Fabrication of regenerated cellulose fibers using phosphoric acid plus hydrogen peroxide treated wheat straw in DMAc/LiCl solvent system
Authors: WANG QING , Zhao Heng , Zhao Li , Huang Mei , Tian Dong , Deng Shihuai , Hu Jinguang , Zhang Xinquan , Shen Fei 
Published: September 2022

Abstract
Regenerated cellulose fibers (RCFs) are widely used biodegradable and eco-friendly materials made from woody biomass to replace synthetic fibers. This work explored the feasibility of fabricating RCFs using phosphoric acid plus hydrogen peroxide (PHP) treated wheat straw in N,N-dimethylacetamide/lithium chloride (DMAc/LiCl) solvent system. The effect of upstream pretreatment conditions on the obtained cellulose-enriched fraction (CEF), the solubility of CEF in DMAc/LiCl, the RCFs’ mechanical properties, and the interfacial interaction of residual lignin were systematically studied. CEF could be completely dissolved in DMAc/LiCl, and its wet-spun RCFs own tensile strength of 184 MPa, the toughness of 25 MJ/m3, and it demonstrated remarkable wet-strength (84 MPa), wet-toughness (11 MJ/m3), and dyeing properties. The existence of residual lignin negatively affected its mechanical properties but would enhance its resistance to water infiltration. The present work provides a new approach for producing RCFs from woody biomass.
Ecological Impact and Human Health Risk Assessment of Pumpkin and Spinach Cultivated around Non- mining Axes of Asu River Group
Authors: Akpa Chibuike , Alu Matthew Okpani , Anih Chioma Benita , Obasi Philip Njoku 
Published: September 2022

Abstract

The study evaluated human health risks associated with the consumption of pumpkin and spinach cultivated around non-mining areas of the Asu River Group. Eighteen samples were collected and analyzed for As, Pb, and Cd, obtaining fifty-four results. The samples were washed with deionized water, air-dried and oven dries at 105°C for 48 hours and analyzed using Varian (USA) Atomic Absorption Spectrophotometer. The concentration of Pb, As, and Cd in the vegetable trend follows; Ebonyi south (ES) < Ebonyi central (EC) < Ebonyi north (EN); EN < ES < EC and ES < EC < EN respectively both for Pumpkin and Spinach vegetables. The Cd, As and Pb, content was below WHO/FAO, (2007) and EU, (2006) specific limits. The daily intake of metals, health risk index, and targeted health quotient were computed. The DIM of Pb, As and Cd were below their respective oral reference dose (RfD) of 0.004, 0.003, and 0.001mg/kg/day. Pb, As, and Cd HRI values for adults, teenagers, and children are in the following order Adult < Teenagers < Children for all samples across the study area. The Pb abundance follows ES < EN < EC in both pumpkin and spinach, and Cd follows EN < EC < EN and EN < ES < EC for pumpkin and spinach respectively. Arsenic HRI in order of EN < EC < EN and EN < ES < EC for pumpkin and spinach respectively. On average, HRI of Pb, As and Cd ranges from 0.00312 to 0.0663, 0.00411 to 0.238 and 0.009 to 0.331 mg/kg/bw/day for the vegetables. The HRI and THQ for adults and teenagers were all < 1 for As, and Pb while Cd THQ > 1 in pumpkin from ES.