bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glycolysis induced by METTL14-related m6A methylation is essential for macrophage function and tumor progression in cervical cancer
Authors: Wang Bingyu , Jiao Xinlin , Zhang Teng , Liu Qingqing , Ye Jinwen , Feng Yuan , Cui Baoxia 
Published: August 2022

Abstract
Cervical cancer is one of the most common tumors of women’s diseases. N6-methyladenosine (m6A) is an abundant RNA modification of mammalian mRNAs and plays a vital role in many diseases, especially tumors. In this study, we aimed to investigate the roles of m6A on macrophage function and tumor progression in cervical cancer. We measured the levels of m6A expression in cervical cancer tissues, and revealed biological functions of METTL14 (Methyltransferase14, N6-Adenosine-Methyltransferase Subunit) on tumor-associated macrophages (TAMs) and PD-1 expression. We found the METTL14-related methylation of m6A in cervical cancer was correlated with infiltration of TAMs, polarization status of infiltrated TAMs, and survival outcomes of cervical cancer patients. Mechanistically, lactate produced by tumor glycolysis in the acidic immunosuppressive tumor microenvironment (TME) has an important role as a proinflammatory and immunosuppressive mediator in this program. METTL14-related methylation of m6A may play a predictive role in the polarization and PD-1 expression of macrophages in TME. The study intends to reveal the effect of m6A on the immune microenvironment and macrophage differentiation of cervical cancer, which could be a viable therapeutic target for the treatment of advanced human cancers.
The lysosome membrane protein, Sidt2: A novel insulin granule membrane protein regulates β cell dedifferentiation by a new pathway involving insulin secretion
Authors: Gao Jialin , Gu Jing , Qi Meng-xiang , Hu Xing , Song Ying-ying , Liu Hai-jun , Zhang Ya-ting , Wu Wen-xiu , Wu Ya-jun , Xu Jia-hao , Wang Jun-hao , Li Jing-rong , Liu Miao-miao , Pei Wen-jun , Zhang Yao , Wang Li-zhuo 
Published: August 2022

Abstract
β-cell dedifferentiation is important in the pathogenesis of type 2 diabetes, and the relationship between lysosome membrane proteins and pancreatic β-cell dedifferentiation is still unknown. Sidt2 is a lysosomal membrane protein identified in our previous study. After Sidt2 elimination, mice exhibited a diabetic phenotype. In the present study, we found that Sidt2 also can be considered a new insulin granule membrane protein. Sidt2 deficiency resulted in islet dysfunction and inhibited insulin secretion. The absence of Sidt2 also led to increased β cell dedifferentiation in mice. Further experiments showed that defects in insulin secretion caused dedifferentiation in the absence of Sid2. In summary, our study has found a new dedifferentiation regulatory mechanism mediated by a lysosomal membrane protein. This new secretory regulation pathway will enrich our understanding of the regulatory mechanisms involved in β cell dedifferentiation and provide new insights into the pathogenesis of diabetes.
Understanding of Bacterial Lignin Extracellular Degradation Mechanisms by Pseudomonas putida KT2440 via Secretomic Analysis
Authors: Xu Zhangyang , Peng Bo , Kitata Reta Birhanu , Nicora Carrie D. , Weitz Karl K. , Pu Yunqiao , Shi Tujin , Cort John R. , Ragauskas Arthur J. , Yang Bin 
Published: August 2022

Abstract
Background: Bacterial lignin degradation is believed to be primarily achieved by a secreted enzyme system. Effects of such extracellular enzyme systems on lignin structural changes and degradation pathways are still not clearly understood, which remains as a bottleneck in the bacterial lignin bioconversion process. Results: This study investigated lignin degradation using an isolated secretome secreted by Pseudomonas putida KT2440 that grew on glucose as the only carbon source. Enzyme assays revealed that the secretome harbored oxidase and peroxidase/Mn2+-peroxidase capacity and reached the highest activity at 120 h of the fermentation time. The degradation rate of alkali lignin was found to be only 8.1% by oxidases, but increased to 14.5% with the activation of peroxidase/Mn2+-peroxidase. Gas chromatography-mass spectrometry (GC-MS) and two-dimensional 1H-13C heteronuclear single-quantum coherence (HSQC) NMR analysis revealed that the oxidases exhibited strong C-C bond (β-β, β-5, and β-1) cleavage. The activation of peroxidases enhanced lignin degradation by stimulating C-O bond (β-O-4) cleavage, resulting in increased yields of aromatic monomers and dimers. Further mass spectrometry-based quantitative proteomics measurements comprehensively identified different groups of enzymes particularly oxidoreductases in P. putida secretome, including reductases, peroxidases, monooxygenases, dioxygenases, oxidases, and dehydrogenases, potentially contributed to the lignin degradation process. Conclusions: Overall, we discovered that bacterial extracellular degradation of alkali lignin to vanillin, vanillic acid, and other lignin derived-aromatics involved a series of oxidative cleavage, catalyzed by active DyP-type peroxidase, multicopper oxidase, and other accessory enzymes. These resutls will guide further metabolic engineering design to improve the efficiency of lignin bioconversion.
Design of an Unmanned Aerial Vehicle for Emergency Situations
Authors: Khan Nafiz Ahmed , Jahan Nusrat , Rayhan Saiaf Bin , Salam Md. Abdus 
Published: August 2022

Abstract
Current research deals with the design, constructions, and prospective applications of a radio-controlled Unmanned Aerial Vehicle (UAV) which was manufactured for the TÜBİTAK UAV TURKEY competition. The motivation behind this research was for possible applications in emergencies (fire, natural disaster, or medical emergencies) due to its remote controllability, maneuverability, and payload carrying capacity. In the conceptual design phase, the individual mission requirements were converted to design requirements, and the UAV's empty weight was estimated after that. Power and wing loading were determined based on the mission profile for the preliminary design. The X-Foil code was then used to compute the aerodynamic efficiency of the airfoil section, after which the wing, fuselage, and empennage were designed. For the detailed design, the structural analysis along the wing span and aircraft performance of the each mission was carried out. Following that, a stability analysis (longitudinal, lateral, and directional) was performed to ensure the UAV's overall stability in the event of any disturbances, and the wing's aerodynamic capabilities were assessed using computational fluid dynamics. Finally, the weight of each component and its corresponding center of gravity (C.G.) were calculated to determine the UAV's most forward and aft centers of gravity.
Temperature-Dependent Enhanced Speciation in Ecosystems with Conserved Symmetries
Authors: Bernido Christopher C. 
Published: August 2022

Abstract
The impact of climate change on biodiversity needs to be understood from a multidisciplinary approach. Using an analytical framework, we investigate the species response to rising temperatures. Common traits and characteristics among species that allow classification at different taxonomic levels imply an underlying symmetry that gives rise to invariances behind the biodiversity observed in nature. Changing temperatures that go beyond a critical limit break this underlying symmetry which could lead to enhanced speciation.
PERBANDINGAN METODE ALGORITMA C4.5 DAN NAÏVE BAYES UNTUK MEMPREDIKSI KELULUSAN MAHASISWA
Authors: Rahmayanti Aulia , Rusdiana Lili , Suratno Suratno 
Published: August 2022

This study was conducted to compare the accuracy of two algorithm methods, namely the C4.5 algorithm and Naïve Bayes algorithm on a number of datasets. The sources of datasets used are student data of the Informatics Engineering Study Program (STMIK Palangkaraya) where each dataset has a different amount of data (instances) and number of attributes. Based on the results of the comparison study of the C4.5 and Naïve Bayes Algorithm for Predicting the On-time Graduation of STMIK Palangkaraya Students, the results obtained from the accuracy of the two algorithms show that the accuracy of the C4.5 Algorithm is 90% better than the Naïve Bayes algorithm, which is only 85%. The C4.5 algorithm also gives values on recall and precision of 92% and 94% better than the Naïve Bayes algorithm with values of 86% and 93%, respectively.
Legionella colonisation in hot water systems in care homes from two Danish municipalities
Authors: Nielsen Niss Skov , Uldum Søren Anker 
Published: August 2022

Abstract
Legionnaires' disease is a serious health risk among the elderly. Water systems in care homes are therefore of particular interest. We investigated the levels of culturable Legionella in the hot water systems in care homes in two Danish municipalities. Two hundred and sixty-eight water samples from 98 care homes were evaluated. Contents of culturable Legionella counts were calculated, and correlations between temperature and colony-forming units (CFU/L) were analysed. Seventy-seven and 81%, respectively, of the care homes were colonised with Legionella in the two municipalities. Most care homes had less than 1,000 CFU/L, but 13 and 16% had more than 10,000 CFU/L. When including first flush samples, 27% of the care homes in Municipality 1 had Legionella levels above 10,000 CFU/L. Temperatures of ≥50 °C in Municipality 1 and ≥55 °C in Municipality 2 correlated with low levels of Legionella. The content of Legionella colonies was significantly higher in care homes in Municipality 1. However, a significantly higher proportion of taps in Municipality 2 had Legionella colonies. In conclusion, temperatures should be raised to 55 °C to avoid high Legionella levels. Test procedures should be evaluated, and the regular use of taps and routine testing for Legionella should be taken into consideration.
Efficient use of liquid digestate in microalgae cultivation for high biomass production and nutrient recovery
Authors: Akkaya Ebru , Can-Güven Emine 
Published: August 2022

Abstract
In this study, different pre-treatment methods were applied to liquid digestate (LD) for the growth of the mixed microalgal culture. In addition, nutrient removal in the LD was investigated. Dilution, filtration, and centrifugation were applied as pre-treatment methods. Microalgal growth was evaluated as dry weight (DW) and nutrient removal was investigated with the analysis of ammonium (NH4+-N) and phosphate (PO4−3-P). Microalgal DW constantly increased throughout the experiment and reached the maximum values of 4.24, 4.02, and 1.47 g/L in centrifuged, diluted, and filtered medium, respectively. Maximum NH4+ (97.7%) and PO4−3 (50.4%) removal efficiency was observed in the centrifuged LD. Based on the results of this study, the optimum growth medium for microalgal growth was centrifuged LD but this culture can be cultivated in diluted LD up to 40-fold. Results also showed that using LD for microalgae production is a suitable application for both nutrient supply for microalgal growth and nutrient recovery from LD.
Activated persulfate by iron-carbon micro electrolysis used for refractory organics degradation in wastewater: a review
Authors: Chen Yu , Gao Yanjiao , Liu Tingting , Zhang Zhao , Li Weishi 
Published: August 2022

Abstract
With the rapid economic development, the discharge of industrial wastewater and municipal wastewater containing many refractory organic pollutants is increasing, so there is an urgent need for processes that can treat refractory organics in wastewater. Iron-carbon micro electrolysis and advanced oxidation based on persulfate radicals (SO4−·) have received much attention in the field of organic wastewater treatment. Iron-carbon micro electrolysis activated persulfate (Fe-C/PS) treatment of wastewater is characterized by high oxidation efficiency and no secondary pollution. This paper reviews the mechanism and process of Fe-C/PS, degradation of organics in different wastewater, and the influencing factors. In addition, the degradation efficiency and optimal reaction conditions (oxidant concentration, catalyst concentration, iron-carbon material, and pH) of Fe-C/PS in the treatment of refractory organics in wastewater are summarized. Moreover, the important factors affecting the degradation of organics by Fe-C/PS are presented. Finally, we analyzed the challenges and the prospects for the future of Fe-C/PS in application, and concluded that the main future directions are to improve the degradation efficiency and cost by synthesizing stable and efficient catalysts, optimizing process parameters, and expanding the application scope.
A Comparison Between Clinical Guidelines and Real-World Treatment Data in Examining the Use of Session Summaries: Retrospective Study
Authors: Sadeh-Sharvit Shiri , Rego Simon A , Jefroykin Samuel , Peretz Gal , Kupershmidt Tomer 
Published: August 2022

BackgroundAlthough behavioral interventions have been found to be efficacious and effective in randomized clinical trials for most mental illnesses, the quality and efficacy of mental health care delivery remains inadequate in real-world settings, partly owing to suboptimal treatment fidelity. This “therapist drift” is an ongoing issue that ultimately reduces the effectiveness of treatments; however, until recently, there have been limited opportunities to assess adherence beyond large randomized controlled trials.ObjectiveThis study explored therapists’ use of a standard component that is pertinent across most behavioral treatments—prompting clients to summarize their treatment session as a means for consolidating and augmenting their understanding of the session and the treatment plan.MethodsThe data set for this study comprised 17,607 behavioral treatment sessions administered by 322 therapists to 3519 patients in 37 behavioral health care programs across the United States. Sessions were captured by a therapy-specific artificial intelligence (AI) platform, and an automatic speech recognition system transcribed the treatment meeting and separated the data to the therapist and client utterances. A search for possible session summary prompts was then conducted, with 2 psychologists validating the text that emerged.ResultsWe found that despite clinical recommendations, only 54 (0.30%) sessions included a summary. Exploratory analyses indicated that session summaries mostly addressed relationships (n=27), work (n=20), change (n=6), and alcohol (n=5). Sessions with meeting summaries were also characterized by greater therapist interventions and included greater use of validation, complex reflections, and proactive problem-solving techniques.ConclusionsTo the best of our knowledge, this is the first study to assess a large, diverse data set of real-world treatment practices. Our findings provide evidence that fidelity with the core components of empirically designed psychological interventions is a challenge in real-world settings. The results of this study can inform the development of machine learning and AI algorithms and offer nuanced, timely feedback to providers, thereby improving the delivery of evidence-based practices and quality of mental health care services and facilitating better clinical outcomes in real-world settings.
The Effect of Gender-Affirming Hormone Therapy on Measures of Kidney Function
Authors: Krupka Emily , Curtis Sarah , Ferguson Thomas , Whitlock Reid , Askin Nicole , Millar Adam C. , Dahl Marshall , Fung Raymond , Ahmed Sofia B. , Tangri Navdeep , Walsh Michael , Collister David 
Published: August 2022

Background and objectives
Gender-affirming hormone therapy modifies body composition and lean muscle mass in transgender persons. We sought to characterize the change in serum creatinine, other kidney function biomarkers, and GFR in transgender persons initiating masculinizing and feminizing gender-affirming hormone therapy.

Design, setting, participants, & measurements
We searched PubMed, EMBASE, the Cochrane Library, and ClinicalTrials.gov from inception to September 16, 2020 for randomized controlled trials, observational studies, and case series that evaluated the change in serum creatinine, other kidney function biomarkers, and GFR before and after the initiation of gender-affirming hormone therapy in adult transgender persons. Two reviewers independently screened and abstracted data, and disagreements were resolved by a third reviewer. A random effects meta-analysis was performed to determine the change in outcomes over follow-up of 3, 6, and 12 months.

Results
Of the 4758 eligible studies, 26 met the inclusion criteria, including nine studies that recruited 488 transgender men and 593 women in which data were meta-analyzed. There was heterogeneity in study design, populations, gender-affirming hormone therapy routes, and dosing. At 12 months after initiating gender-affirming hormone therapy, serum creatinine increased by 0.15 mg/dl (95% confidence interval, 0.00 to 0.29) in 370 transgender men and decreased by −0.05 mg/dl (95% confidence interval, −0.16 to 0.05) in 361 transgender women. No study reported the effect of gender-affirming hormone therapy on albuminuria, proteinuria, cystatin C, or measured GFR.

Conclusions
Gender-affirming hormone therapy increases serum creatinine in transgender men and does not affect serum creatinine in transgender women. The effect on gender-affirming hormone therapy on other kidney function biomarkers and measured GFR is unknown.

Clinical Trial registry name and registration number:
Change in Kidney Function Biomarkers in Transgender Persons on Gender Affirmation Hormone Therapy–A Systematic Review and Meta-Analysis, CRD42020214248
Fundamento teórico epistémico del diagnóstico cráneo-cervico maxilofacial
Authors: Ramos Montiel Ronald Roossevelt 
Published: August 2022

Desde los inicios del conocimiento, el hombre ha procurado acercarse a la realidad en la búsqueda de conocer, comprender, controlar y/o predecir el entorno que lo rodea; en tal suceso, el conocimiento puede adquirirse de manera vulgar (doxa) u opinión empírica como experiencias personales, científica trascendiendo los hechos de la realidad o de manera filosófica con la esencia y finalidades del mundo. En tal contexto, el análisis filosófico de la naturaleza del conocimiento relacionado con las características raciales y morfológicas de las poblaciones es un tema muy discutido desde tiempos inmemorables, así también, es uno de los temas sin resultados completamente definidos debido a la incesante búsqueda de la categorización y a la constante idealización de una clasificación de las características morfológicas que nos unen a la vez que nos diferencian.
PENGARUH PENAMBAHAN BERBAGAI BAHAN TAMBAHAN (SINGKONG, PEPAYA, NASI AKING) DALAM BERBAGAI PERBANDINGAN TERHADAP KUALITAS TEMPE CAMPURAN SEBAGAI MEDIA LEAFLET MATERI BIOTEKNOLOGI SMA KELAS XII
Authors: Bisyria Fatih , Zaenab Siti , Rofieq Ainur 
Published: August 2022

ABSTRAKTujuan penelitian ini adalah untuk mengetahui pengaruh penambahan bahan tambahan (singkong, pepaya, nasi aking) dan perbandingannya yang paling optimal terhadap kadar protein, kadar air, dan organoleptik pada tempe campuran. Jenis penelitian yang digunakan adalah penelitian True Experiment Design dan Studi Pengembangan. Design yang digunakan adalah Faktorial Contras Ortogonal Design. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) pola faktorial, dengan faktor pertama jenis bahan (kedelai-singkong, kedelai-pepaya, kedelai-nasi aking) dan faktor kedua perbandingan kedelai: bahan tambahan (50%:50%, 60%:40%, 70%:30%). Data berupa kadar protein, kadar air, dan organoleptik pada tempe. Teknik Analisis data yang digunakan adalah Analisis Varian Dua Faktor dan Uji Beda Jarak Nyata Duncan (BJND) 5%. Hasil Penelitian menunjukkan bahwa jenis bahan dan perbandingan tidak berpengaruh terhadap kadar protein, kadar air, dan organoleptik pada tempe campuran. Kadar protein pada keseluruhan tempe campuran masih berada di atas standar SNI yaitu minimal 16,00 %. Tempe kontrol (kedelai murni) memiliki kandungan protein paling tinggi dengan nilai 19,37%. Pada tempe campuran yang memiliki kadar protein paling tinggi yaitu tempe campuran nasi aking dengan kadar protein 18,42 %. Kadar air pada keseluruhan tempe campuran masih berada di bawah standar SNI yaitu maksimal 65,00 %. Tempe kontrol (kedelai murni) memiliki kadar air paling rendah dengan nilai 46,63%. Pada tempe campuran yang memiliki kadar air paling rendah yaitu tempe campuran nasi aking dengan kadar air 47,73 %. Bahan tambahan nasi aking berpengaruh paling baik terhadap sifat organoleptik tempe campuran yaitu dengan rerata nilai 4,93 (sangat suka). Kata Kunci: Tempe Campuran, Bahan Tambahan, Kadar Protein, Kadar Air, Organoleptik
KEANEKARAGAMAN MAKROFAUNA TANAH DAERAH PERTANIAN APEL SEMI ORGANIK DAN PERTANIAN APEL NON ORGANIK KECAMATAN BUMIAJI KOTA BATU SEBAGAI BAHAN AJAR BIOLOGI SMA
Authors: Aminullah Yusron , Mahmudati Nurul , Zaenab Siti 
Published: August 2022

ABSTRAKJenis penelitian yang digunakan adalah penelitian deskriftif kuantitatif. Penelitian ini dilakukan pada tanggal 20-23 April 2014 dengan metode plot (kuadrat) dan menggunakan jebakan (Pittfall Trap). Penelitian dilakukan dengan bertujuan untuk mengetahui jenis makrofauna tanah, parameter ekologi, indeks keanekaragaman jenis, dan indeks kemerataan jenis yang terdapat pada pertanian apel semi organik dan pertanian apel non organik. Hasil penelitian menunjukkan bahwa makrofauna tanah yang ditemukan terdiri dari jenis Platydracus sp, Phyllophoga sp, Geophilus sp, Helicodiscus sp, Gryllus sp, Gryllotalpa gryllotalpa, Pirata piraticus, Pisaurina mira, Megascolex sp, Sigmoria trimaculata, Pachycodyla sp, Anoplolepis gracilipes, Iridomyrmex sp, Camponotus sp, dan Pheidole moerens. Parameter ekologi makrofauna tanah adalah: (1) Kepadatan berkisar antara 2,667 ind/m2 - 416,889 ind/m2, kepadatan relatif berkisar antara 0,004 - 0,487. kepadatan terendah jenis Pisaurina mira dan tertinggi jenis Irydomyrmex sp. (2) Frekuensi berkisar antara 0,063 - 0,844, frekuensi relatif berkisar antara 0,010 – 0,176 dengan nilai frekuensi terenda jenis Phyllophaga sp dan tertinggi jenis Irydomyrmex sp. (3) Indeks nilai penting tertinggi jenis Irydomyrmex sp 0,687, terendah Pisaurina mira 0,011. Indeks keanekaragaman jenis Shannon wiener (H’) 1,719 atau kategori keanekaragaman jenis rendah. Nilai evennes (E) yaitu 0,62 yang berarti populasi cenderung merata (E mendekati 1). Kelimpahan berkisar 1 - 16,63, terendah jenis Pisaurina mira dan tertinggi jenis Irydomyrmex sp.(4) Hasil penelitian ini digunakan sebagai bahan ajar Biologi SMA dalam bentuk poster. Kata kunci: Makrofauna tanah, pertanian apel, dan keanekaragaman jenis.