bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regeneration of collagen fibrils at the papillary dermis by reconstructing basement membrane at the dermal–epidermal junction
Authors: Iriyama Shunsuke , Ogura Yuki , Nishikawa Saori , Hosoi Junichi , Amano Satoshi 
Published: January 2022

AbstractThe epidermal basement membrane deteriorates with aging. We previously reported that basement membrane reconstruction not only serves to maintain epidermal stem/progenitor cells in the epidermis, but also increases collagen fibrils in the papillary dermis. Here, we investigated the mechanism of the latter action. Collagen fibrils in the papillary dermis were increased in organotypic human skin culture treated with matrix metalloproteinase and heparinase inhibitors. The expression levels of COL5A1 and COL1A1 genes (encoding collagen type V α 1 chain and collagen type I α 1 chain, respectively) were increased in fibroblasts cultured with conditioned medium from a skin equivalent model cultured with the inhibitors and in keratinocytes cultured on laminin-511 E8 fragment-coated plates. We then examined cytokine expression, and found that the inhibitors increased the expression of PDGF-BB (platelet-derived growth factor consisting of two B subunits) in epidermis. Expression of COL5A1 and COL1A1 genes was increased in cultured fibroblasts stimulated with PDGF-BB. Further, the bifunctional inhibitor hydroxyethyl imidazolidinone (HEI) increased skin elasticity and the thickness of the papillary dermis in the skin equivalent. Taken together, our data suggests that reconstructing the basement membrane promotes secretion of PDGF-BB by epidermal keratinocytes, leading to increased collagen expression at the papillary dermis.
Direct evidence of proximal tubular proliferation in early diabetic nephropathy
Authors: Uehara-Watanabe Noriko , Okuno-Ozeki Natsuko , Minamida Atsushi , Nakamura Itaru , Nakata Tomohiro , Nakai Kunihiro , Yagi-Tomita Aya , Ida Tomoharu , Ikeda Kisho , Kitani Takashi , Yamashita Noriyuki , Kamezaki Michitsugu , Kirita Yuhei , Matoba Satoaki , Tamagaki Keiichi , Kusaba Tetsuro 
Published: January 2022

AbstractKidney hypertrophy is a common clinical feature in patients with diabetes and is associated with poor renal outcomes. Initial cell proliferation followed by cellular hypertrophy are considered the responsible mechanisms for diabetic kidney hypertrophy. However, whether similar responses against hyperglycemia continue in the chronic phase in diabetes is unclear. We performed lineage tracing analysis of proximal tubular epithelia using novel type 2 diabetic mice with a tamoxifen-inducible proximal tubule-specific fluorescent reporter. Clonal analysis of proximal tubular epithelia demonstrated that the labeled epithelia proliferated in type 2 diabetic mice. Based on the histological analysis and protein/DNA ratio of sorted labeled tubular epithelia, there was no evidence of cellular hypertrophy in type 2 diabetic mice. Lineage tracing and histological analyses of streptozocin-induced type 1 diabetes also revealed that cellular proliferation occurs in the chronic phase of type 1 diabetes induction. According to our study, epithelial proliferation accompanied by SGLT2 upregulation, rather than cellular hypertrophy, predominantly occurs in the hypertrophic kidney in both type 1 and type 2 diabetes. An increased number of SGLT2+ tubular epithelia may be an adaptive response against hyperglycemia, and linked to the hyper-reabsorption of sodium and glucose observed in type 2 diabetes patients.
Therapeutic potential of highly functional codon-optimized microutrophin for muscle-specific expression
Authors: Starikova Anna V. , Skopenkova Victoria V. , Polikarpova Anna V. , Reshetov Denis A. , Vassilieva Svetlana G. , Velyaev Oleg A. , Shmidt Anna A. , Savchenko Irina M. , Soldatov Vladislav O. , Egorova Tatiana V. , Bardina Maryana V. 
Published: January 2022

AbstractHigh expectations have been set on gene therapy with an AAV-delivered shortened version of dystrophin (µDys) for Duchenne muscular dystrophy (DMD), with several drug candidates currently undergoing clinical trials. Safety concerns with this therapeutic approach include the immune response to introduced dystrophin antigens observed in some DMD patients. Recent reports highlighted microutrophin (µUtrn) as a less immunogenic functional dystrophin substitute for gene therapy. In the current study, we created a human codon-optimized µUtrn which was subjected to side-by-side characterization with previously reported mouse and human µUtrn sequences after rAAV9 intramuscular injections in mdx mice. Long-term studies with systemic delivery of rAAV9-µUtrn demonstrated robust transgene expression in muscles, with localization to the sarcolemma, functional improvement of muscle performance, decreased creatine kinase levels, and lower immunogenicity as compared to µDys. An extensive toxicity study in wild-type rats did not reveal adverse changes associated with high-dose rAAV9 administration and human codon-optimized µUtrn overexpression. Furthermore, we verified that muscle-specific promoters MHCK7 and SPc5-12 drive a sufficient level of rAAV9-µUtrn expression to ameliorate the dystrophic phenotype in mdx mice. Our results provide ground for taking human codon-optimized µUtrn combined with muscle-specific promoters into clinical development as safe and efficient gene therapy for DMD.
Engineering surgical stitches to prevent bacterial infection
Authors: Vieira Daniela , Angel Samuel N. , Honjol Yazan , Masse Maude , Gruenheid Samantha , Harvey Edward J. , Merle Geraldine 
Published: January 2022

AbstractSurgical site infections (SSIs) account for a massive economic, physiological, and psychological burden on patients and health care providers. Sutures provide a surface to which bacteria can adhere, proliferate, and promote SSIs. Current methods for fighting SSIs involve the use of sutures coated with common antibiotics (triclosan). Unfortunately, these antibiotics have been rendered ineffective due to the increasing rate of antibiotic resistance. A promising new avenue involves the use of metallic nanoparticles (MNPs). MNPs exhibit low cytotoxicity and a strong propensity for killing bacteria while evading the typical antibiotic resistance mechanisms. In this work, we developed a novel MNPs dip-coating method for PDS-II sutures and explored the capabilities of a variety of MNPs in killing bacteria while retaining the cytocompatibility. Our findings indicated that our technique provided a homogeneous coating for PDS-II sutures, maintaining the strength, structural integrity, and degradability. The MNP coatings possess strong in vitro antibacterial properties against P aeruginosa and S. aureus—varying the %of dead bacteria from ~ 40% (for MgO NPs) to ~ 90% (for Fe2O3) compared to ~ 15% for uncoated PDS-II suture, after 7 days. All sutures demonstrated minimal cytotoxicity (cell viability > 70%) reinforcing the movement towards the use MNPs as a viable antibacterial technology.
Superior frontal regions reflect the dynamics of task engagement and theta band-related control processes in time-on task effects
Authors: Yu Shijing , Mückschel Moritz , Beste Christian 
Published: January 2022

AbstractImpairment of cognitive performance is often observed in time-on tasks. Theoretical considerations suggest that especially prefrontal cortex cognitive control functions is affected by time-on-task effects, but the role of effort/task engagement is not understood. We examine time-on-task effects in cognitive control on a neurophysiological level using a working-memory modulated response inhibition task and inter-relate prefrontal neuroanatomical region-specific theta-band activity with pupil diameter data using EEG-beamforming approaches. We show that task performance declines with time-on tasks, which was paralleled by a concomitant decreases of task-evoked superior frontal gyrus theta-band activity and a reduction in phasic pupil diameter modulations. A strong relation between cognitive control-related superior frontal theta-band activity and effort/task engagement indexed by phasic pupil diameter modulations was observed in the beginning of the experiment, especially for tasks requiring inhibitory controls and demanding high working memory. This strong relation vanished at the end of the experiment, suggesting a decoupling of cognitive control resources useable for a task and effort invested that characterizes time-on-task effects in prefrontal cortical structures.
Spatially mapping thermal transport in graphene by an opto-thermal method
Authors: Braun Oliver , Furrer Roman , Butti Pascal , Thodkar Kishan , Shorubalko Ivan , Zardo Ilaria , Calame Michel , Perrin Mickael L. 
Published: January 2022

AbstractMapping the thermal transport properties of materials at the nanoscale is of critical importance for optimizing heat conduction in nanoscale devices. Several methods to determine the thermal conductivity of materials have been developed, most of them yielding an average value across the sample, thereby disregarding the role of local variations. Here, we present a method for the spatially resolved assessment of the thermal conductivity of suspended graphene by using a combination of confocal Raman thermometry and a finite-element calculations-based fitting procedure. We demonstrate the working principle of our method by extracting the two-dimensional thermal conductivity map of one pristine suspended single-layer graphene sheet and one irradiated using helium ions. Our method paves the way for spatially resolving the thermal conductivity of other types of layered materials. This is particularly relevant for the design and engineering of nanoscale thermal circuits (e.g. thermal diodes).
Breaking the crosstalk of the Cellular Tumorigenic Network by low-dose combination therapy in lung cancer patient-derived xenografts
Authors: Gürgen Dennis , Conrad Theresia , Becker Michael , Sebens Susanne , Röcken Christoph , Hoffmann Jens , Langhammer Stefan 
Published: January 2022

AbstractNon-small cell lung cancer (NSCLC) is commonly diagnosed at advanced stages limiting treatment options. Although, targeted therapy has become integral part of NSCLC treatment therapies often fail to improve patient’s prognosis. Based on previously published criteria for selecting drug combinations for overcoming resistances, NSCLC patient-derived xenograft (PDX) tumors were treated with a low dose combination of cabozantinib, afatinib, plerixafor and etoricoxib. All PDX tumors treated, including highly therapy-resistant adeno- and squamous cell carcinomas without targetable oncogenic mutations, were completely suppressed by this drug regimen, leading to an ORR of 81% and a CBR of 100%. The application and safety profile of this low dose therapy regimen was well manageable in the pre-clinical settings. Overall, this study provides evidence of a relationship between active paracrine signaling pathways of the Cellular Tumorigenic Network, which can be effectively targeted by a low-dose multimodal therapy to overcome therapy resistance and improve prognosis of NSCLC.
Flexibility provision of residential energy hubs with demand response applications
Authors: Ostovar Sara , Moeini‐Aghtaie Moein , Hadi Mohammad Behzad 
Published: January 2022

AbstractSmart grids help local distribution companies (LDCs) facilitate the communication between grid operators and residential prosumers. While the prosumers are striving to optimize their daily load profile by home energy management systems (HEMSs) deployment, the LDCs attempt to enhance the operation of the grid in an efficient way and decrease network losses. This paper attempts to develop a new bi‐level probabilistic optimization framework wherein an HEMS optimizes its respective daily load profile, and determines its flexibility provision, which is communicated to an LDC. In the proposed framework, a decentralized approach is used to achieve the flexibility product, which is the modulation of energy, through the incentive‐based demand response (DR) programs. Finally, the LDC applies the prosumer's flexibility' offers to optimize its operational performance. The two‐point estimate method (2PEM) is employed to model the uncertainties. The applicability of the framework is demonstrated by applying it to a system with one residential feeder.
Recurrent neural network non‐singular terminal sliding mode control for path following of autonomous ground vehicles with parametric uncertainties
Authors: Xing Benhua , Xu Enyong , Wei Jin , Meng Yanmei 
Published: January 2022

AbstractUncertain characteristics along with unknown exterior disturbances are fundamental issues in the path‐following control of autonomous ground vehicles (AGVs). Here, a novel robust non‐singular terminal sliding mode (NTSM) control method based on a recurrent neural network (RNN) structure is proposed to enhance the path‐following performance of AGVs. First, based on the dynamic model and path‐following model of AGVs, the robust NTSM steering controller is proposed to ensure that the lateral offset converges to zero within a limited time, and concurrently suppresses the chattering phenomenon that occurs in the regular sliding mode control (SMC) strategy. Then, the RNN is applied to approximate the unknown dynamics section of the system online. With an implied feedback loop, its study ability precedes the regular neural network to catch up with the dynamic response. In addition, the adaptive learning algorithms of the parameters in the RNN are derived using the Lyapunov stability theorem and Taylor linearisation technique to ensure the stability of the closed‐loop system. Finally, the robustness and effectiveness of the proposed control approach against parametric uncertainties and unknown external disturbances are verified in the Simulink‐CarSim simulation platform by comparison with selected reported control methods.
Stellar dating using chemical clocks and Bayesian inference
Authors: Moya A. , Sarro L. M. , Delgado-Mena E. , Chaplin W. J. , Adibekyan V. , Blanco-Cuaresma S. 
Published: January 2022

Context. Dating stars is a major challenge with a deep impact on many astrophysical fields. One of the most promising techniques for this is using chemical abundances. Recent space- and ground-based facilities have improved the quantity of stars with accurate observations. This has opened the door for using Bayesian inference tools to maximise the information we can extract from them.
Aims. Our aim is to present accurate and reliable stellar age estimates of FGK stars using chemical abundances and stellar parameters.
Methods. We used one of the most flexible Bayesian inference techniques (hierarchical Bayesian models) to exceed current possibilities in the use of chemical abundances for stellar dating. Our model is a data-driven model. We used a training set that has been presented in the literature with ages estimated with isochrones and accurate stellar abundances and general characteristics. The core of the model is a prescription of certain abundance ratios as linear combinations of stellar properties including age. We gathered four different testing sets to assess the accuracy, precision, and limits of our model. We also trained a model using chemical abundances alone.
Results. We found that our age estimates and those coming from asteroseismology, other accurate sources, and also with ten Gaia benchmark stars agree well. The mean absolute difference of our estimates compared with those used as reference is 0.9 Ga, with a mean difference of 0.01 Ga. When using open clusters, we reached a very good agreement for Hyades, NGC 2632, Ruprecht 147, and IC 4651. We also found outliers that are a reflection of chemical peculiarities and/or stars at the limit of the validity ranges of the training set. The model that only uses chemical abundances shows slightly worse mean absolute difference (1.18 Ga) and mean difference (−0.12 Ga).
The strange case of Na I in the atmosphere of HD 209458 b
Authors: Morello G. , Casasayas-Barris N. , Orell-Miquel J. , Pallé E. , Cracchiolo G. , Micela G. 
Published: January 2022

Aims. We aim to investigate the origin of the discrepant results reported in the literature about the presence of Na I in the atmosphere of HD 209458 b, based on low- and high-resolution transmission spectroscopy.
Methods. We generated synthetic planetary atmosphere models and we compared them with the transmission light curves and spectra observed in previous studies. Our models account for the stellar limb-darkening and Rossiter-McLaughlin (RM) effects, and contemplate various possible scenarios for the planetary atmosphere.
Results. We reconciled the discrepant results by identifying a range of planetary atmospheres that are consistent with previous low- and high-resolution spectroscopic observations. Either both datasets are interpreted as consistent with a total absence of Na I in the planetary atmosphere (with Hubble Space Telescope data being affected by limb darkening), or the terminator temperature of HD 209458 b has to have an upper limit of about 1000 K. In particular, we find that 1D transmission spectra with lower-than-equilibrium temperatures can also explain the previously reported detection of absorption signal at low resolution due to differential transit depth in adjacent bands, while the cores of the Na I D lines may be masked by the strong RM signal seen at high resolution. We also rule out high-altitude clouds, which would otherwise mask the absorption signal at low resolution, as the source of the discrepancies.
Conclusions. This work highlights the synergies between different observing techniques, specifically low- and high-resolution spectroscopy, to fully characterise transiting exoplanet systems.
The BINGO project
Authors: Fornazier Karin S. F. , Abdalla Filipe B. , Remazeilles Mathieu , Vieira Jordany , Marins Alessandro , Abdalla Elcio , Santos Larissa , Delabrouille Jacques , Mericia Eduardo , Landim Ricardo G. , Ferreira Elisa G. M. , Barosi Luciano , Queiroz Amilcar R. , Villela Thyrso , Wang Bin , Wuensche Carlos A. , Costa Andre A. , Liccardo Vincenzo , Paiva Novaes Camila , Peel Michael W. , dos Santos Marcelo V. , Zhang Jiajun 
Published: January 2022

Context.Observing the neutral hydrogen distribution across the Universe via redshifted 21 cm line intensity mapping constitutes a powerful probe for cosmology. However, the redshifted 21 cm signal is obscured by the foreground emission from our Galaxy and other extragalactic foregrounds. This paper addresses the capabilities of the BINGO survey to separate such signals.Aims.We show that the BINGO instrumental, optical, and simulations setup is suitable for component separation, and that we have the appropriate tools to understand and control foreground residuals. Specifically, this paper looks in detail at the different residuals left over by foreground components, shows that a noise-corrected spectrum is unbiased, and shows that we understand the remaining systematic residuals by analyzing nonzero contributions to the three-point function.Methods.We use the generalized needlet internal linear combination, which we apply to sky simulations of the BINGO experiment for each redshift bin of the survey. We use binned estimates of the bispectrum of the maps to assess foreground residuals left over after component separation in the final map.Results.We present our recovery of the redshifted 21 cm signal from sky simulations of the BINGO experiment, including foreground components. We test the recovery of the 21 cm signal through the angular power spectrum at different redshifts, as well as the recovery of its non-Gaussian distribution through a bispectrum analysis. We find that non-Gaussianities from the original foreground maps can be removed down to, at least, the noise limit of the BINGO survey with such techniques.Conclusions.Our component separation methodology allows us to subtract the foreground contamination in the BINGO channels down to levels below the cosmological signal and the noise, and to reconstruct the 21 cm power spectrum for different redshift bins without significant loss at multipoles 20 ≲ ℓ ≲ 500. Our bispectrum analysis yields strong tests of the level of the residual foreground contamination in the recovered 21 cm signal, thereby allowing us to both optimize and validate our component separation analysis.
Small-scale magnetic fields of the spectroscopic binary T Tauri stars V1878 Ori and V4046 Sgr
Authors: Hahlin A. , Kochukhov O. 
Published: January 2022

Aims. The goal of this study is to investigate the small-scale magnetic fields of the two spectroscopic binary T Tauri stars V1878 Ori and V4046 Sgr. This is done to complete the observational characterisation of the surface magnetic fields of these stars because only their large-scale magnetic fields have been studied with Zeeman Doppler imaging (ZDI) so far.
Methods. To investigate the small-scale magnetic fields, the differential Zeeman intensification of near-infrared Ti I lines was investigated using high-resolution archival spectra obtained with the ESPaDOnS spectrograph at the CFHT. In order to study the binary components separately, the spectra were disentangled by considering observations taken at different orbital phases. The Zeeman-intensification analysis was performed based on detailed polarised radiative transfer calculations aided by the Markov chain Monte Carlo inference, treating magnetic field filling factors and other stellar parameters that could affect the spectra as free parameters.
Results. The obtained average magnetic field strengths of the components of V1878 Ori are 1.33 ± 0.08 and 1.57 ± 0.09 kG, respectively. Previous ZDI studies of V1878 Ori recovered about 14 and 20% of this total magnetic field strength. For V4046 Sgr, the magnetic field strengths are 1.96 ± 0.18 and 1.83 ± 0.18 kG, respectively. In this case, about 12 and 9% of the total magnetic field strength was detected by ZDI.
Conclusions. The small-scale magnetic field strengths obtained from Zeeman intensification are similar for the two components of each binary. This is in contrast to the large-scale magnetic fields obtained from ZDI investigations, performed using the same observations, which found that magnetic field strengths and topologies vary significantly in the components of the two binaries. While the large-scale field might look significantly different, the overall magnetic energy, primarily carried by the small-scale magnetic fields, appears to be quite similar. This indicates that the efficiency of the magnetic dynamo is comparable for the components of the two binaries.