bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physalis angulata leaf extract ameliorates L-NG-nitroarginine methyl ester (L-NAME)-induced preeclampsia symptoms in rats through improved endothelial progenitor cells and endothelial cells due to reduced antiangiogenic factor and oxidative stress
Authors: Nugrahenny Dian , Rudijanto Achmad , Permatasari Nur , Wiyasa I Wayan Arsana , Widodo Mohammad Aris , Mintaroem Karyono , Widjajanto Edi , Mustofa Mustofa 
Published: July 2022

Background: This study aimed to determine the ameliorative effects of Physalis angulata leaf extract on L-NG-nitroarginine methyl ester (L-NAME)-induced preeclampsia symptoms in rats. Methods: Phytochemical analysis of the extract was performed with liquid chromatography-high resolution mass spectrometry (LC-HRMS). Pregnant Wistar rats were randomly divided into five groups (n=6). Preeclampsia rats were injected with L-NAME on gestation days 9 to 18 (G9–G18), while sham rats were injected with the same vehicle volume. Three groups of preeclampsia rats were orally supplemented with the extract at doses of 500, 1500, and 2500 mg/kg body weight/day on G12–G18. The tail-cuff method measured blood pressures. Spectrophotometry determined urine protein levels. Serum soluble fms-like tyrosine kinase (sFlt)-1 levels were evaluated using an enzyme-linked immunosorbent assay (ELISA). Serum and placental malondialdehyde (MDA) levels, superoxide dismutase (SOD) activities, and nitric oxide (NO) levels were measured by colorimetry. Immunohistochemistry was used to determine tail artery endothelial nitric oxide synthase (eNOS), placental eNOS, and placental hypoxia-inducible factor (HIF)-1α expressions. Circulating angiogenic cells (CACs) and endothelial colony-forming cells (ECFCs) were counted using flow cytometry. Results: L-NAME injection increased blood pressures, 24-h total urine protein level, serum sFlt-1 level, serum and placental MDA levels, percentages of CACs and ECFCs, and placental HIF-1α expression. It also decreased serum and placental SOD activities, serum NO level, tail artery and placental eNOS expressions compared to the sham group. Physalis angulata leaf extract administration lowered blood pressures, urine protein level, sFlt-1 level, MDA levels, percentages of CACs and ECFCs, and placental HIF-1α expression. The extract increased SOD activities, NO level, tail artery and placental eNOS expressions compared to the preeclampsia group. Conclusions: Physalis angulata leaf extract reduces antiangiogenic factor and oxidative stress. It also enhances eNOS/NO signaling. Thus, it improves EPC and endothelial cell function and reverses L-NAME-induced hypertension and proteinuria in preeclampsia rats.
Why did he say that? Teaching physicians-in-training how to recognize hidden emotions in end-of-life prognosis conversations: an autoethnography
Authors: Stonestreet John 
Published: July 2022

Background: Intuitively accessible via WhyDidHeSayThat.com, this article begins with two unconscionable end-of-life prognosis-related quotes from best-selling physician-author, Atul Gawande, and an unnamed doctor, asking: “Why did he say that?” The article then proceeds to answer this question by showing how physicians’ most common end-of-life communication blunders are rooted in their unexplored emotions. Healthcare’s only widespread communication training focused on examining the role of hidden emotions in influencing the flow of conversation is found in Spiritual Care’s “Verbatim” education modules. While the need for physicians’ emotional self-awareness for improved end-of-life communication has been identified in the literature, no one has explored how this need might be met by custom-tailoring Spiritual Care’s “Verbatim” education modules for physicians-in-training. Methods: This article utilizes the qualitative research method of autoethnography to grant physicians access to the content and power of Spiritual Care’s “Verbatim” education modules for identifying hidden emotions in clinical communication. Results: Using a profound personal example from the author’s firsthand experience of the suggested training tool, the “Verbatim” module is shown to grant revelatory self-knowledge and invaluable emotional intelligence. The same model then illuminates the physician cases. Conclusion: Spiritual Care’s “Verbatim” education modules address universal issues of clinical communication and emotional self-awareness that are applicable to physician-patient/family conversations surrounding end-of-life decision-making. Customizing these communication modules for physicians-in-training may help to address physicians’ emotionally-triggered conversational miscues in end-of-life prognosis communication. Existing programs for complementary end-of-life communication training are noted, and it is claimed that a combination of each of these models, together with the proposed module, may be ideal. It is also admitted that no form of education or training can ensure ethical communication. Therefore the ultimate solution is to supplement communication training with real-time, third-party support and accountability. This can be achieved by the "Doctor Body Cam" intervention protocol, accessible via DoctorBodyCam.com.
Approaches to integrating online videos into health professions curricula: educators’ perspectives from multiple institutions
Authors: Aluri Kelly , Sow Mohamed , Amieva Manuel , Chen Sharon 
Published: July 2022

Background: The COVID-19 pandemic has accelerated a transition from lecture-based classes to blended and online learning, increasing the need to integrate publicly available online educational videos. Although online videos are widely available, it is challenging for educators to effectively integrate them into a curriculum. Years before the pandemic, educators from different institutions integrated videos from a library of microbiology and immunology resources into different curricula. Their experiences may inform current educators on the approach to incorporating external resources into their unique curricula. Methods: We interviewed US health professions instructors or course directors who had previously requested access to online microbiology and immunology videos. Using thematic analysis, we organized prominent themes into an existing framework for curriculum development. We then reflected on the meaning of the themes using the same conceptual framework. Results: We found that educators from different schools were able to integrate the same publicly available videos into varying contexts. Most used them as preparation for interactive sessions. For integrating videos, educators felt success when the following actions occurred. 1) Educators integrated videos as a tool to enhance active-learning activities. 2) Educators created activities that focused on clinical applications of knowledge, taught critical thinking, and developed enthusiasm for the subject. 3) They tested students on knowledge application and major concepts rather than solely on content for high-stakes exams. 4) Educators worked with administrators who understood the goals of integrating external videos and supported educators with time and resources to develop effective blended learning. Conclusion: Our study suggests that educators integrating external resources into their curricula may benefit from first establishing their goals and aspirations for their students. These goals then become the anchor for other curricular elements, including external videos, in-class activities, and assessments. Our study highlights the need for dedicated time to develop experienced and enthusiastic educators.
Specimen and sample metadata standards for biodiversity genomics: a proposal from the Darwin Tree of Life project
Authors: Lawniczak Mara K.N. , Davey Robert P. , Rajan Jeena , Pereira-da-Conceicoa Lyndall L. , Kilias Estelle , Hollingsworth Peter M. , Barnes Ian , Allen Heather , Blaxter Mark , Burgin Josephine , Broad Gavin R. , Crowley Liam M. , Gaya Ester , Holroyd Nancy , Lewis Owen T. , McTaggart Seanna , Mieszkowska Nova , Minotto Alice , Shaw Felix , Richards Thomas A. , Sivess Laura A.S. ,   Darwin Tree of Life Consortium
Published: July 2022

The vision of the Earth BioGenome Project1 is to complete reference genomes for all of the planet’s ~2M described eukaryotic species in the coming decade. To contribute to this global endeavour, the Darwin Tree of Life Project (DToL2) was launched in 2019 with the aim of generating complete genomes for the ~70k described eukaryotic species that can be found in Britain and Ireland. One of the early tasks of the DToL project was to determine, define, and standardise the important metadata that must accompany every sample contributing to this ambitious project. This ensures high-quality contextual information is available for the associated data, enabling a richer set of information upon which to search and filter datasets as well as enabling interoperability between datasets used for downstream analysis. Here we describe some of the key factors we considered in the process of determining, defining, and documenting the metadata required for DToL project samples. The manifest and Standard Operating Procedure that are referred to throughout this paper are likely to be useful for other projects, and we encourage re-use while maintaining the standards and rules set out here.
Celiac disease - a common autoimmune disease with significantly delayed diagnosis
Authors: Choina Magdalena , Kinga Pożarowska  , Rudziński Gracjan , Cukrowska Bożena , Majsiak Emilia 
Published: July 2022

Introduction: Celiac disease (CD) is an autoimmune disease that affects genetically predisposed individuals. In course of the disease, consumption of gluten causes damage to the small intestine. Due to various clinical manifestations, diagnosing CD poses a challenge to clinicians. It has been proven by several study groups that the diagnostic delay in CD is still too long and provokes severe health complications.
Purpose: The aim of the study is to highlight the importance of diagnostic delay in CD, its consequences and possible solutions.
Description of the state of knowledge: The diagnosis of CD is based on the clinical picture, serological test, duodenal mucosal biopsies and genetic tests. Many cases of CD remain undiagnosed in spite of published guidelines for CD diagnosis. Consequently, the diagnosis is significantly delayed: the mean duration of the diagnostic process in Poland was 7.3 years. In other countries, patients the time from the onset of the symptoms to establishing CD diagnosis was up to 10 years. The diagnostic delay leads to reduced quality of life and the development of severe complications, such as neoplastic disease.
Summary: Diagnostic delay in CD is an issue of great importance. Because of the reduced quality of life and the possibility of neoplasm, it is crucial to take action in order to shorten the diagnostic process of CD.
Report on a scoping review of nursing interventions for young people's psychological wellbeing
Authors: Turner James Bradley , Cooper Lucy , Woodward Amie , Painter Jon , Day Pat , Hazelby Gayle , Harrop Deborah , Moore Kerry 
Published: July 2022

Aim: This paper reports on a two-part mixed methods scoping project, funded by the RCN Foundation, to review the available evidence and undertake a Delphi study of nurse-led interventions. Nurses in the field were surveyed to understand their role in promoting children and young people's mental health and emotional wellbeing across the UK. Methods: A scoping review of the literature was undertaken and a two-part Delphi survey was developed and managed. Findings: Results of the literature review were inconclusive. There was a paucity of literature, particularly in the UK, suggesting that nurses may be under-represented in the research seeking to make recommendations concerning their profession. 244 responses to the Delphi method survey were received. Responses confirmed the fundamental importance of the therapeutic relationship. They also suggested that nurse-led pre-crisis and early interventions which addressed the mental health and emotional wellbeing needs of children and young people were being extensively utilised. All countries and all regions appeared to have primarily community-based services. School-based services were perceived to be highly beneficial. Early interventions typically targeted levels of resilience, emotional regulation and the prevention of ongoing mental health problems. Respondents viewed these interventions as effective but often encountered organisational and practical challenges, including access to training and ongoing supervision.
Undergraduate education of trauma and orthopaedic surgery in the UK
Authors: Poacher Arwel T. , Bhachoo Hari , Weston Jack , Shergill Kavita , Poacher Gethin , Froud Joe 
Published: July 2022

Aims Evidence exists of a consistent decline in the value and time that medical schools place upon their undergraduate orthopaedic placements. This limited exposure to trauma and orthopaedics (T&O) during medical school will be the only experience in the speciality for the majority of doctors. This review aims to provide an overview of undergraduate orthopaedic training in the UK. Methods This review summarizes the relevant literature from the last 20 years in the UK. Articles were selected from database searches using MEDLINE, EMBASE, ERIC, Cochrane, and Web of Science. A total of 16 papers met the inclusion criteria. Results The length of exposure to T&O is declining; the mean total placement duration of two to three weeks is significantly less than the four- to six-week minimum advised by most relevant sources. The main teaching methods described in the literature included didactic lectures, bedside teaching, and small group case-based discussions. Students preferred interactive, blended learning teaching styles over didactic methods. This improvement in satisfaction was reflected in improvements in student assessment scores. However, studies failed to assess competencies in clinical skills and examinations, which is consistent with the opinions of UK foundation year doctors, approximately 40% of whom report a “poor” understanding of orthopaedics. Furthermore, the majority of UK doctors are not exposed to orthopaedics at the postgraduate level, which only serves to amplify the disparity between junior and generalist knowledge, and the standards expected by senior colleagues and professional bodies. Conclusion There is a deficit in undergraduate orthopaedic training within the UK which has only worsened in the last 20 years, leaving medical students and foundation doctors with a potentially significant lack of orthopaedic knowledge. Cite this article: Bone Jt Open 2022;3(7):549–556.
Association between Low Back Pain and Work and Lifestyle Variables among Fruit Farm Workers: A Cross-Sectional Study
Authors: Beltran Picón Sol Patricia , dos Passos Muana Hiandra Pereira , Ferraz Zalane Cristina Marques , Franco Sara Rúbia de Souza , de Araújo Rodrigo Cappato , Locks Francisco 
Published: July 2022

HighlightsLow back pain has a high prevalence among fruit farm workers.Fruit packing workers are more likely to have low back pain than field workers.Abstract. The objective of this observational and correlational cross-sectional study was to analyze the association of labor variables, lifestyle, and low back pain among workers on a grape farm in the northeast region of Brazil. A total of 180 rural workers, men and women, age 18 years and older, and active on the company’s staff, participated in the study. The presence of low back pain, considered as a dependent variable, was categorized as yes or no, and the independent variables were personal, work, and lifestyle factors. The workers performed functions in the field (72%) and fruit packing (28%). Low back pain was reported by 44.1% (n = 79) of the participants. There were associations between low back pain and the function performed by the worker. Thus, workers in fruit packing were 1.53 more likely to have low back pain (95% CI 1.09-2.15) when compared with workers in the field, demonstrating the need for strategies to improve the structure of the workday, such as the inclusion of active breaks. Keywords: Low back pain, Musculoskeletal discomfort, Occupational health.
Estimating the Nonfatal Injury Undercount in Agriculture from 2004 to 2019
Authors: Picciotto Isabelle , Beatty Timothy , Hill Alexandra Estvan 
Published: July 2022

HighlightsGovernment estimates of nonfatal occupational injuries and illnesses understate the hazards in U.S. agriculture.From 2004 to 2019, government estimates only captured 13% to 26% of the true number of nonfatal injuries.Estimates of nonfatal injuries are more accurate for crop production than for animal production.Estimates are more accurate over time, with a decline in self-employed and unpaid family labor in agriculture.Abstract. The U.S. Bureau of Labor Statistics provides annual estimates of nonfatal occupational injuries and illnesses by U.S. industry sector. We performed a series of corrections to these estimates for each year from 2004 through 2019 to account for institutional and behavioral drivers of the undercount in the sample used to construct these estimates for the U.S. agricultural industry. Institutional factors consisted of the exclusion of small farms and self-employed and family workers, as well as the employment undercount due to the highly seasonal nature of agricultural work. Behavioral factors consisted of willful and negligent underreporting by employers. We updated the estimates using information on the number of people employed in the excluded portions of the agricultural industry and estimates of the underreporting rate from prior work. Over this period, we show that the government estimates only captured 13% to 26% of the true number of nonfatal injuries and illnesses, missing 74% to 87% of the true case counts each year. The government estimates were more accurate for crop production, missing an average of 77% of cases, than for animal production, missing an average of 83% of cases. Willful and negligent underreporting was the largest contributor to the undercount, followed by the exclusion of self-employed and unpaid family workers. Keywords: Agriculture, Nonfatal injuries and illnesses, Occupational injuries, Survey of Occupational Injuries and Illnesses, Undercount.
Twisted weakly coupled relative humidity sensor coated with a graphene oxide/polyvinyl alcohol composite
Authors: Sun Yuqing , Guo Jiachen , Xiao Yinfeng , Fan Junfeng , Chen Jigang , Zhao Dong , Jia Bo 
Published: July 2022

A new coupled fiber optic humidity sensor based on a double-tapered fiber twisted weakly coupled structure coated with a graphene oxide/polyvinyl alcohol (GO/PVA) film has been reported for the first time, to the best of our knowledge. The sensor adopts a

2

×

2

coupler structure with a waist diameter of 20 µm. The GO/PVA composite film is coated in the weakly coupled area to increase the sensitivity of the sensor. The thickness of the coating layer is about 3 µm. The sensor can realize linear sensing in the relative humidity (RH) range of 45%–85%RH with a dynamic response time of 1.9 s and a recovery time of 5.7 s. The sensitivity of the sensor is up to 0.002/%RH, and the linearity of the sensor is as high as 98.65%. Moreover, the sensor has good stability, reversibility, and low-temperature crosstalk.
Diaphragm-free gas pressure sensor based on all-sapphire fiber Fabry–Perot interferometers
Authors: Feng Xinxing , Jiang Yi , Gao Hongchun , Tang Caijie , Wang Xuefeng 
Published: July 2022

An all-sapphire fiber external Fabry–Perot interferometer (EFPI) sensor for measuring gas pressure is proposed and investigated. The sensor head is manufactured from a sapphire fiber ferrule and sapphire tube, and the same material can ensure the stability of the sensor structure at a high temperature. The refractive index of the gas is linearly related to the gas pressure. Therefore, the gas pressure can be measured by studying the optical cavity length of the EFPI. A multi-stage coupled multimode fiber is used to pick up the interference signal of the fiber EFPI. The pressure response of the sensor at different temperatures was measured in the experiment. The experimental results show that the sapphire fiber EFPI can measure 0–5 MPa gas pressure in the environment of 17–1400°C. The sensitivity of the sensor decreases with the increasing temperature, and the maximum sensitivity is 1.1673 µm/MPa (20°C). The sensor is compact and suitable for gas pressure measurement at a high temperature.
Associations between low Apgar scores and mortality by race in the United States: A cohort study of 6,809,653 infants
Authors: Gillette Emma , Boardman James P. , Calvert Clara , John Jeeva , Stock Sarah J. 
Published: July 2022

Background
Apgar scores measure newborn health and are strongly associated with infant outcomes, but their performance has largely been determined in primarily white populations. Given the majority of the global population is not white, we aim to assess whether the association between low Apgar score and mortality in infants varies across racial groups.

Methods and findings
Population-based cohort study using 2016 to 2017 United States National Vital Statistics System data. The study included singleton infants born between 37+0 and 44+6 weeks to mothers over 15 years, without congenital abnormalities. We looked at 3 different mortality outcomes: (1) early neonatal mortality; (2) overall neonatal mortality; and (3) infant mortality. We used logistic regression to assess the association between Apgar score (categorized as low, intermediate, and normal) and each mortality outcome, and adjusted for gestational age, sex, maternal BMI, education, age, previous number of live births, and smoking status, and stratified these models by maternal race group (as self-reported on birth certificates). The cohort consisted of 6,809,653 infants (52.8% non-Hispanic white, 23.7% Hispanic, 13.8% non-Hispanic black, 6.6% non-Hispanic Asian, and 3.1% non-Hispanic other). A total of 6,728,829 (98.8%) infants had normal scores, 63,467 (0.9%) had intermediate scores, and 17,357 (0.3%) had low Apgar scores. Compared to infants with normal scores, low-scoring infants had increased odds of infant mortality. There was strong evidence that this association varied by race (p < 0.001) with adjusted odds ratios (AORs) of 54.4 (95% confidence interval [CI] 49.9 to 59.4) in non-Hispanic white, 70.02 (95% CI 60.8 to 80.7) in Hispanic, 23.3 (95% CI 20.3 to 26.8) in non-Hispanic black, 100.4 (95% CI 74.5 to 135.4) in non-Hispanic Asian, and 26.8 (95% CI 19.8 to 36.3) in non-Hispanic other infants. The main limitation was missing data for some variables, due to using routinely collected data.

Conclusions
The association between Apgar scores and mortality varies across racial groups. Low Apgar scores are associated with mortality across racial groups captured by United States (US) records, but are worse at discriminating infants at risk of mortality for black and non-Hispanic non-Asian infants than for white infants. Apgar scores are useful clinical indicators and epidemiological tools; caution is required regarding racial differences in their applicability.