bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design, synthesis, and herbicidal activity of indole-3-carboxylic acid derivatives as potential transport inhibitor response 1 antagonists
Authors: Wang Xing , Luo Mu-Jia , Wang Yu-Xuan , Han Wen-Qing , Miu Jian-Xin , Luo Xi-Ping , Zhang Ai-Dong , Kuang Yi 
Published: July 2022

Auxins as an important class of phytohormones play essential roles in plant life cycle; therefore, developing compounds with auxin-like properties for plant growth regulation and weed control applications is of great significance. Herein, we reported the design, synthesis, and herbicidal activity evaluation of a series of novel indole-3-carboxylic acid derivatives as auxin receptor protein TIR1 antagonists. Petri dish herbicidal activity assay demonstrated that most of the as-synthesized target compounds exhibited good-to-excellent inhibition effects (60–97% inhibitory rates) on roots and shoots of both dicotyledonous rape (B. napus) and monocotyledonous barnyard grass (E. crus-galli). The inhibition rates of compounds 10d and 10h reached up to 96% and 95% for the root of rape (B. napus) at 100 mg/L, and they also maintained 92% and 93% inhibition rates even if at 10 mg/L, respectively. Molecular docking revealed that the interactions between these synthesized target compounds and TIR1 protein include tight π–π stacking, hydrogen bond, and hydrophobic interactions. This work expands the range of auxin chemistry for the development of new auxin mimic herbicides.
Comparison of the ocular surface microbiota between thyroid-associated ophthalmopathy patients and healthy subjects
Authors: Ji Xuan , Dong Kui , Pu Ji , Yang Jing , Zhang Zhaoxia , Ning Xiaoling , Ma Qin , Kang Zhiming , Xu Jianguo , Sun Bin 
Published: July 2022

PurposeThyroid-associated ophthalmopathy (TAO) is a chronic autoimmune disease. In this study, high-throughput sequencing was used to investigate the diversity and composition of the ocular microbiota in patients with TAO.MethodsPatients with TAO did not receive treatment for the disease and did not have exposed keratitis. Patients with TAO (TAO group) and healthy individuals (control group) were compared. All samples were swabbed at the conjunctival vault of the lower eyelid. The V3 to V4 region of the 16S rDNA was amplified using polymerase chain reaction and sequenced on the Illumina HiSeq 2500 Sequencing Platform. Statistical analysis was performed to analyze the differences between the groups and the correlation between ocular surface microbiota and the disease. The ocular surface microbiota of patients and healthy individuals were cultured.ResultsThe ocular surface microbiota structure of TAO patients changed significantly. The average relative abundance of Bacillus and Brevundimonas increased significantly in the TAO group. Corynebacterium had a significantly decreased relative abundance (P&lt;0.05). Paracoccus, Haemophilus, Lactobacillus, and Bifidobacterium were positively correlated with the severity of clinical manifestations or disease activity (P&lt;0.05). Bacillus cereus and other opportunistic pathogens were obtained by culture from TAO patients.ConclusionsThis study found that the composition of ocular microbiota in patients with TAO was significantly different from that in healthy individuals. The ocular surface opportunistic pathogens, such as Bacillus, Brevundimonas, Paracoccus, and Haemophilus in TAO patients, increase the potential risk of ocular surface infection. The findings of this study provide a new avenue of research into the mechanism of ocular surface in TAO patients.
High prevalence of Rickettsia spp. in ticks from wild hedgehogs rather than domestic bovine in Jiangsu province, Eastern China
Authors: Qi Yong , Ai Lele , Jiao Jun , Wang Junhu , Wu Deping , Wang Pengcheng , Zhang Guoyu , Qin Yong , Hu Cheng , Lv Ruichen , Lu Nianhong , Zhu Changqiang , Mao Yingqing , Qi Rui , Li Yuexi , Tan Weilong 
Published: July 2022

BackgroundSpotted fever group Rickettsia (SFGR), containing various pathogenic Rickettsia spp., poses remarkable negative influences to public health by causing various severe or mild diseases. Information regarding prevalence of SFGR in ticks in Jiangsu province, Eastern China, is still limited and needs urgent investigations.MethodsHedgehog- and bovine-attached ticks were collected from Jiangsu province, Eastern China. DNA of individual ticks was extracted for nested polymerase chain reaction amplifications targeting gltA, 16S ribosomal RNA (rrs), ompA, ompB, and sca4 genes following with sequencing. SFGR-specific IgG antibodies in sera of local donators were evaluated using ELISA.ResultsOverall, 144 (83.2%) of the 173 ticks from hedgehogs and 2 (1.2%) of the 168 ticks from bovine were positive for one of the three identified Rickettsia spp., with significant difference between the two groups (P = 3.6e-52). Candidatus Rickettsia principis (9; 5.2%) and R. heilongjiangensis (135; 78.0%) were detected in Haemaphysalis flava rather than in H. longicornis ticks from hedgehogs. R. heilongjiangensis (1; 0.6%) and Candidatus R. jingxinensis (or Candidatus R. longicornii) (1; 0.6%) were identified in H. longicornis and Rhipicephalus microplus ticks from bovine, respectively. Phylogenetic analysis indicated Candidatus R. jingxinensis belonged to R. japonica subgroup, whereas Candidatus R. principis belonged to a novel subgroup. Higher serological prevalence of spotted fever and SFGR-specific IgG antibody level in humans were observed around the investigated area than in urban areas, without significant difference.ConclusionCandidatus R. principis and Candidatus R. jingxinensis were identified in Jiangsu province, Eastern China, and fully genetically characterized for the first time. The higher prevalence of SFGR in hedgehog-attached ticks as well as the higher SFGR-specific IgG antibody level and seropositive rate in humans around the investigated area suggested that more attention should be paid to SFGR. This pathogen is usually transmitted or harbored by wild animals and ticks. This study provides important epidemiological data for both physicians and public health officers in developing early prevention and control strategies against potential Rickettsia infections and in the preparation of suitable testing and treatment needs for rickettsiosis in the endemic areas.
Trajectory of low-density lipoprotein cholesterol in patients with chronic kidney disease and its association with cardiovascular disease
Authors: Wang Shih-Wei , Li Lung-Chih , Fu Chung-Ming , Lee Yueh-Ting , Kuo Hsiao-Ching , Hsu Chien-Ning 
Published: July 2022

BackgroundThe role of longitudinal temporal trends in LDL-C in cardiovascular disease (CVD) in patients with chronic kidney disease (CKD) and diabetes is unclear. This study categorized the long-term LDL-C trajectory and determined its association with the incidence of atherosclerotic CVD in patients with CKD according to diabetes status and estimated glomerular filtration rate (eGFR).MethodsThe risk of atherosclerotic CVD was estimated in 137,127 Taiwanese patients with CKD using six LDL-C trajectory classes determined by the latent class mixed model as optimal, near optimal, above optimal, borderline, sustained high, and declined high over 5 years.ResultsThe risk of CVD was higher in the sustained high LDL-C [&gt;160 mg/dL over time; adjusted hazard ratio (aHR) = 1.68, 95% CI = 1.45–1.94], declined high LDL-C (&gt;160 to &lt;100 mg/dL; aHR = 1.23, 95% CI = 1.11–1.38), and borderline LDL-C (approximately 140 mg/dL over time; aHR = 1.16, 95% CI = 1.07–1.26) groups than in the optimal LDL-C group (&lt;100 mg/dL over time). There was no such association in patients with an eGFR &lt;15 mL/min/1.73 m2. Persistent diabetes was associated with a 1.15–2.47-fold increase in CVD in patients with high LDL-C (&gt;120 mg/dL).ConclusionThe LDL-C trajectory pattern was associated with the phenotype of CVD risk. The degree of risk varied according to eGFR and diabetes status. A stable low LDL-C over time was potentially beneficial for prevention of CVD. Intensive lipid management and periodic assessment of LDL-C is essential to reduce the risk of CVD in patients with CKD and diabetes.
Explainable machine learning models for predicting 30-day readmission in pediatric pulmonary hypertension: A multicenter, retrospective study
Authors: Duan Minjie , Shu Tingting , Zhao Binyi , Xiang Tianyu , Wang Jinkui , Huang Haodong , Zhang Yang , Xiao Peilin , Zhou Bei , Xie Zulong , Liu Xiaozhu 
Published: July 2022

BackgroundShort-term readmission for pediatric pulmonary hypertension (PH) is associated with a substantial social and personal burden. However, tools to predict individualized readmission risk are lacking. This study aimed to develop machine learning models to predict 30-day unplanned readmission in children with PH.MethodsThis study collected data on pediatric inpatients with PH from the Chongqing Medical University Medical Data Platform from January 2012 to January 2019. Key clinical variables were selected by the least absolute shrinkage and the selection operator. Prediction models were selected from 15 machine learning algorithms with excellent performance, which was evaluated by area under the operating characteristic curve (AUC). The outcome of the predictive model was interpreted by SHapley Additive exPlanations (SHAP).ResultsA total of 5,913 pediatric patients with PH were included in the final cohort. The CatBoost model was selected as the predictive model with the greatest AUC for 0.81 (95% CI: 0.77–0.86), high accuracy for 0.74 (95% CI: 0.72–0.76), sensitivity 0.78 (95% CI: 0.69–0.87), and specificity 0.74 (95% CI: 0.72–0.76). Age, length of stay (LOS), congenital heart surgery, and nonmedical order discharge showed the greatest impact on 30-day readmission in pediatric PH, according to SHAP results.ConclusionsThis study developed a CatBoost model to predict the risk of unplanned 30-day readmission in pediatric patients with PH, which showed more significant performance compared with traditional logistic regression. We found that age, LOS, congenital heart surgery, and nonmedical order discharge were important factors for 30-day readmission in pediatric PH.
Modification effect of changes in cardiometabolic traits in association between kidney stones and cardiovascular events
Authors: Xu Min , Zhao Zhiyun , Shen Feixia , Hu Ruying , Lu Jieli , Xu Yu , Wang Tiange , Li Mian , Chen Gang , Chen Li , Chen Lulu , Chen Yuhong , Deng Huacong , Gao Zhengnan , Huo Yanan , Li Qiang , Liu Chao , Luo Zuojie , Mu Yiming , Qin Guijun , Qin Yingfen , Shi Lixin , Su Qing , Wan Qin , Wang Guixia , Wang Shuangyuan , Wang Youmin , Wu Shengli , Xu Yiping , Yan Li , Yang Tao , Ye Zhen , Yu Xuefeng , Zhang Yinfei , Zhao Jiajun , Zeng Tianshu , Wang Weiqing , Bi Yufang , Tang Xulei , Ning Guang 
Published: July 2022

BackgroundsWhether longitudinal changes in metabolic status influence the effect of kidney stones on cardiovascular disease (CVD) remains unclarified. We investigated the modification effect of status changes in metabolic syndrome (MetS) in the association of kidney stones with risk of incident CVD events.MethodsWe performed a prospective association and interaction study in a nationwide cohort including 129,172 participants aged ≥ 40 years without CVDs at baseline and followed up for an average of 3.8 years. Kidney stones information was collected by using a questionnaire and validated by medical records. The repeated biochemical measurements were performed to ascertain the metabolic status at both baseline and follow-up.Results4,017 incident total CVDs, 1,413 coronary heart diseases (CHDs) and 2,682 strokes were documented and ascertained during follow-up. Kidney stones presence was significantly associated with 44%, 70% and 31% higher risk of CVDs, CHDs and stroke, respectively. The stratified analysis showed significant associations were found in the incident and sustained MetS patients, while no significant associations were found in the non-MetS at both baseline and follow-up subjects or the MetS remission ones, especially in women. For the change status of each single component of the MetS, though the trends were not always the same, the associations with CVD were consistently significant in those with sustained metabolic disorders, except for the sustained high blood glucose group, while the associations were consistently significant in those with incident metabolic disorders except for the incident blood pressure group. We also found a significant association of kidney stone and CVD or CHD risk in the remain normal glucose or triglycerides groups; while the associations were consistently significant in those with incident metabolic disorders except for the incident blood pressure group. We also found a significant association of kidney stone and CVD or CHD risk in the remain normal glucose or triglycerides groups.ConclusionsA history of kidney stones in women with newly developed MetS or long-standing MetS associated with increased risk of CVD. The mechanisms link kidney stones and CVD risk in the metabolic and non-metabolic pathways were warranted for further studies.
Late Quaternary changes in moisture availability and weathering intensity on the central Tibetan Plateau indicated by chemical signatures of ostracod shells
Authors: Börner Nicole , Jochum Klaus Peter , Stuhr Marleen , Abstein Michelle , Plessen Birgit , Frenzel Peter , Wang Junbo , Zhu Liping , Schwalb Antje 
Published: July 2022

High-resolution multi-proxy records from two lakes on the southern Tibetan Plateau, Nam Co and Tangra Yumco, are used to infer long-term variations in the Asian monsoon system with a novel set of ostracod shell chemistry proxies. We track the moisture evolution since the Last Glacial Maximum using the trace element, rare earth element (REE) and stable isotope composition of ostracod shells. The sediment records covering the past 18.8 cal. ka BP and 17.4 cal. ka BP, respectively, demonstrate the suitability of REEs as indicators of weathering intensity and thus hydrological changes and moisture sources in the catchment. In Nam Co, high concentrations of light REEs between 14 and 13 cal. ka BP suggest an increased drainage from the glaciated Nyainqêngtanglha Mountains in the south, pointing to meltwater input. REEs in ostracod shells therefore provide additional information on water sources critical for the interpretation of stable isotope records. Mg/Ca, Sr/Ca, and Ba/Ca ratios reflect salinity and thus changes in effective moisture. Asynchronous behavior of Mg/Ca, Sr/Ca, and Ba/Ca ratios are controlled by changes in dominance of precipitating carbonate minerals in the lake. Synchronous behavior reflects calcite precipitation, indicating low-Mg/Ca warm-wet conditions. Constantly low Sr/Ca ratios reflect aragonite precipitation, indicating high-Mg dry conditions. Increased Sr/Ca and Ba/Ca relative to Mg/Ca ratios show monohydrocalcite precipitation, indicating high-Mg/Ca cold-dry conditions. Furthermore, Fe/Ca, Mn/Ca and U/Ca ratios in ostracods reflect changes in oxygen saturation in lake bottom waters controlled by lake level and microbial activity. The paleoclimate histories reconstructed from Nam Co and Tangra Yumco show high similarity throughout the late Quaternary. We identified two major dry periods, corresponding to Heinrich 1 and the Younger Dryas, followed by strengthening in Indian summer monsoon precipitation. The early Holocene is characterized by a moisture maximum, reflecting abundant water supply by a strong ISM. A time-delayed shift to dry conditions occurred at 2.6 cal. ka BP at Tangra Yumco, and at 2 cal. ka BP at Nam Co, resulting in decreasing lake levels, caused by weakened monsoon intensity due to a southeastward migration of the ISM-Westerly boundary with an estimated velocity of approximately 600 m per year.
Northward expansion of the westerlies over glacial southeastern Australia: evidence from semi-arid lunette dunes, temperate basalt plains, and wind modelling
Authors: Fitzsimmons Kathryn E. , Gromov Sergey S. 
Published: July 2022

It has long been hypothesized that the last glacial maximum (LGM) oversaw cold, arid, windy climates across southern Australia, and that these were driven by intensification and northward expansion of mid-latitude westerly circulation. Moreover, it was recently suggested that Australia experienced an extended LGM which began several millennia before the global peak. Aeolian sedimentary deposits provide key evidence for these hypotheses, and climate modelling an alternative means to test them. As yet, however, combined approaches to reconstructing glacial environments on the continent are scarce. Here we provide new evidence for westerly wind regimes across glacial southeastern Australia. We confirm active transverse lunette deposition at c. 29 ka and c. 23–19 ka in the semi-arid Willandra Lakes, and identify aeolian sand incursions to Spring Creek on the temperate Western Victorian Volcanic Plains from c. 29 ka. The Spring Creek deposits contain a surprising quantity of sand-sized quartz given the basalt setting, which we propose to be allochthonous and likely transported some distance. The site lies more than 50 km east and south of dunefields which were active at the same time and may have contributed sediment via long distance transport. We investigate the hypothesis for northward glacial expansion of westerly winds by combining our sediment records with aeolian particle transport simulations. We find that LGM near-surface winds were dominated by stronger, more focussed westerly air flow across southeastern Australia, compared with presently more diffuse wind regimes. Our results suggest stronger potential for LGM eastward distal sand transport onto the basalt plains, coeval with enhanced aeolian activity in the semi-arid Australian dunefields. Our combined reconstruction of aeolian deposition and trajectory modelling confirms the extended LGM hypothesis and indicates a northward migration of westerly winds over southeastern Australia during this period.
LncRNA GAS5 Knockdown Mitigates Hepatic Lipid Accumulation via Regulating MiR-26a-5p/PDE4B to Activate cAMP/CREB Pathway
Authors: Xu Shizan , Wang Yajie , Li Zhengyang , Hua Qian , Jiang Miao , Fan Xiaoming 
Published: July 2022

ObjectiveNon-alcoholic fatty liver disease (NAFLD) can be attributed to the dysregulation of hepatic lipid metabolism; however, its cellular and molecular mechanisms remain unclear. This study aims to explore the effect of long non-coding RNA growth arrest specific 5 (GAS5) on hepatic lipid metabolism in fatty liver models.MethodsObese mice, high fat diet-fed mice and free fatty acid-stimulated cells were used for GAS5 expression detection. GAS5 overexpression or knockdown models were established to elucidate the regulatory function of GAS5 in de novo lipogenesis (DNL) and mitochondrial function. Bioinformatic analyses and dual luciferase assays were used to investigate the interaction between GAS5, miR-26a-5p and phosphodiesterase (PDE) 4B. The involvement of the cyclic adenosine monophosphate (cAMP)/cAMP-response element-binding protein (CREB) pathway was evaluated using H89 and forskolin treatment.ResultsGAS5 was activated in vitro and in vivo fatty liver models. Knockdown of GAS5 reduced lipid droplet accumulation, DNL associated enzymes and preserved mitochondrial function, while GAS5 overexpression exacerbated hepatic lipid accumulation. Mechanistically, GAS5 sponged miR-26a-5p to increase PDE4B expression and subsequently modulated DNL and mitochondrial function via the cAMP/CREB pathway.ConclusionDownregulation of GAS5 can activate the cAMP/CREB pathway through miR-26a-5p/PDE4B axis to mitigate hepatic lipid accumulation. This study provides evidence that downregulation of GAS5 may be a potential therapeutic option for the treatment of NAFLD.
Determinants of Diabetic Peripheral Neuropathy and Their Clinical Significance: A Retrospective Cohort Study
Authors: Cheng Yifan , Cao Wen , Zhang Junzhe , Wang Jiabin , Liu Xiang , Wu Qianqian , Lin Qingxia 
Published: July 2022

BackgroundIn this study, we investigated the epidemiological characteristics and predictors of diabetic peripheral neuropathy (DPN) in adult patients with type 2 diabetes mellitus (DM).MethodsThe study was designed as a retrospective cohort trial at the First Affiliated Hospital of Wenzhou Medical University. From January 2017 to December 2020, a total of 1,262 patients with DM were enrolled to assess the risk factors for DPN. The patients were divided into two groups (DPN group and non-DPN group). The Mann–Whitney U test or t-test, receiver operating characteristic (ROC) analyses, univariate chi-square analyses, and multiple logistic regression analyses were used to analyze the adjusted predictors of DPN.ResultsThe overall prevalence of DPN in DM patients was 72.7% (n = 793/1,091). Multivariate analysis revealed that age &gt; 66 years (odds ratio [OR], 2.647; 95% confidence interval [CI] 1.469–4.770; p = 0.002), history of hypertension (OR, 1.829; 95% CI 1.146–2.920; p = 0.011), neutrophil (NE) levels exceeding 4.0 × 109/L (OR 0.256; 95% CI 0.162–0.405; p = 0.001), lymphocyte (LY) levels over 3.0 × 109/L (OR 7.173; 95% CI 4.258–12.086; p = 0.000), HbA1c &gt; 7.7% (OR 3.151; 95% CI 1.959–5.068; p = 0.000), and FT3 &gt; 4.4 pmol/L (OR 0.417; 95% CI 0.263–0.662; p = 0.000) were six significant predictive factors for the prevalence of DPN.ConclusionsHigh levels of LY, HbA1c, history of hypertension, and &gt; 66 years of age increase the risk of DPN in adult patients with DM, while high levels of NE and FT3 were protective factors of DPN. Thus, the prediction of DPN can significantly be improved by identifying older patients over the age of 66 and history of hypertension, as well as establishing the biochemical cutoff values of NE, LY, HbA1c, and FT3.
Online monitoring and sampling analysis of siltation in the middle route of the south-to-north water diversion project
Authors: Zhang Dawei , Han Xiaolin , Zhong Qiang , Wang Shulei , Ding Liuqian , Liu Yuan , Chen Qigang , Wang Fujun 
Published: July 2022

The middle route project (MRP) of the South to North Water Diversion Project is a significant infrastructure and alleviating water scarcity in Northern China. MRP suffered from untraditional siltation problems. Obvious siltation occurred in the regulating reservoir at the end of the channel and some locations with weak hydrodynamic conditions in the channel when the mineral siltation concentration in the flow is very low. To study the characteristics of the siltation and the siltation time period, an IoT based automatic siltation monitoring system using cloud was installed at the outlet of the inverted siphon project on Xiao River. Three years of online monitoring data since 2018 and the siltation samples at five sites for particle size analysis were collected. The monitoring data shows that siltation mainly occurs during March to October, and almost no siltation occurs in winter. The maximum siltation speed can reach 390 mm per day. The particle size of the siltation gradually increases from upstream to downstream, which mainly occurs in the range above 100 m. The organic matter contained in the siltation shows a significant increase from 40.3 to 86.4% at upstream and downstream sampling position, respectively. Monitoring results shows the main body of the siltation in the MRP is not the traditional siltation but the remnants of the algae that proliferate in large numbers. During March to October, the temperature is suitable for the proliferation of algae which attaches to the sediment particles and gradually grows downstream with the flow.