bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-ganglionic nicotinic acetylcholine receptor α3 subunit antibody as a potential biomarker associated with lupus enteritis
Authors: Aso Kuniyuki , Kono Michihito , Abe Nobuya , Fujieda Yuichiro , Kato Masaru , Atsumi Tatsuya 
Published: February 2022

ABSTRACT

Objectives
We aimed to identify the clinical significance of anti-ganglionic nicotinic acetylcholine receptor α3 subunit (gAChRα3) antibodies (Abs) in patients with systemic lupus erythematosus (SLE).

Methods
This retrospective study comprised adult patients with SLE who visited our hospital from 2006 through 2019. Anti-gAChRα3 Abs were measured in the sera of patients with SLE using a luciferase immunoprecipitation system assay. The clinical features of the patients with or without anti-gAChRα3 Abs were compared. We evaluated whether the Abs predict a specific manifestation and affect its development or relapse rate.

Results
Among 144 patients, anti-gAChRα3 Abs were detected in 29 patients. Lupus enteritis (LE) was more frequently seen in anti-gAChRα3 Ab-positive patients than negative patients. The levels of anti-gAChRα3 Abs were significantly higher in patients with LE than those with other lupus manifestations. Logistic regression analysis revealed the anti-gAChRα3 Abs were independent predictors for LE (odds ratio 6.2, 95% confidence interval 1.9–20.3, p = .002). Kaplan–Meier analysis showed the rate of LE development or relapse from the time of sera collection was higher in anti-gAChRα3 Ab-positive patients than in negative patients (p &lt; .001).

Conclusion
Anti-gAChRα3 Abs could be a predictive biomarker for the development or relapse of LE.
Rheumatoid arthritis caused by non-tuberculous mycobacteria infection
Authors: Takaoka Hirokazu , Shimomura Taizo , Suzushima Hitoshi 
Published: February 2022

Abstract
A 72-year-old Japanese woman had right digital flexor tenosynovitis with a non-tuberculous mycobacteria (NTM) infection, which was identified as Mycobacterium marinum in culture. She had been treated at another hospital with clarithromycin, rifampicin, and ethambutol for the non-tuberculous tenosynovitis. However, the swelling of her right hand worsened, and 5 months later, her left hand swelled and she exhibited symmetrical arthritis. Blood tests detected elevated serum C-reactive protein and rheumatoid factor positivity. Although rheumatoid arthritis (RA) was suspected and corticosteroid treatment was started, she came to our hospital because of the insufficient treatment effect. Musculoskeletal ultrasonography showed intra-articular and peritendinous power Doppler signal-positive symmetrical synovitis. A contrast-enhanced magnetic resonance imaging (MRI) evaluation of the left hand without NTM tenosynovitis revealed findings of inflammatory synovitis accompanied by bone marrow oedema. We diagnosed RA and started treatment with weekly low-dose methotrexate pulses and 2 weeks of tocilizumab administration; her symptoms then disappeared within 2 months. This is a rare case of RA manifested with NTM-associated arthritis.
Flexible TALEs for an expanded use in gene activation, virulence and scaffold engineering
Authors: Becker Sebastian , Mücke Stefanie , Grau Jan , Boch Jens 
Published: February 2022

Abstract
Transcription activator-like effectors (TALEs) are bacterial proteins with a programmable DNA-binding domain, which turned them into exceptional tools for biotechnology. TALEs contain a central array of consecutive 34 amino acid long repeats to bind DNA in a simple one-repeat-to-one-nucleotide manner. However, a few naturally occurring aberrant repeat variants break this strict binding mechanism, allowing for the recognition of an additional sequence with a −1 nucleotide frameshift. The limits and implications of this extended TALE binding mode are largely unexplored. Here, we analyse the complete diversity of natural and artificially engineered aberrant repeats for their impact on the DNA binding of TALEs. Surprisingly, TALEs with several aberrant repeats can loop out multiple repeats simultaneously without losing DNA-binding capacity. We also characterized members of the only natural TALE class harbouring two aberrant repeats and confirmed that their target is the major virulence factor OsSWEET13 from rice. In an aberrant TALE repeat, the position and nature of the amino acid sequence strongly influence its function. We explored the tolerance of TALE repeats towards alterations further and demonstrate that inserts as large as GFP can be tolerated without disrupting DNA binding. This illustrates the extraordinary DNA-binding capacity of TALEs and opens new uses in biotechnology.
A look on food intake and satiety: from humans to rodent models
Authors: Costa Daniela G , Almeida Cláudia , Cavadas Cláudia , Carmo-Silva Sara 
Published: February 2022

Abstract
Satiety is a complex state, influenced by numerous factors that go beyond food ingestion. Satiety influences food habits and behavior, thus affecting human health. This review provides an overview of physiological mechanisms involved in satiety and of methodologies to assess food intake and satiety in both animal models and humans. The following topics are highlighted: differences between satiety and satiation; how the central nervous system regulates food intake and satiety; the impact of different macronutrients on satiety; and how the manipulation of food composition might influence overall satiety. Bringing together knowledge on this myriad of satiety mechanisms and how we can study them is useful to better understand and control obesity and other eating disorders.
Metasomatic Modification of the Mesoarchaean Ulamertoq Ultramafic Body, Southern West Greenland
Authors: Nishio Ikuya , Morishita Tomoaki , Itano Keita , Guotana Juan Miguel , Tamura Akihiro , Szilas Kristoffer , Harigane Yumiko , Tani Kenichiro , Pearson D Graham 
Published: February 2022

AbstractNumerous ultramafic rocks occur as lens-shaped bodies in the Archaean continental crust exposed in southern West Greenland. As some of the oldest exposed ultramafic bodies, determining their origin, as mantle segments or magmatic cumulates, is an important yet controversial issue. The origin of these Archaean ultramafic rocks remains unclear, in-part because these rocks have undergone metasomatic modification since their formation, yet the effects of this metasomatism have so far not been assessed in detail, despite being crucial for understanding their geochemical evolution. Here, we examined the petrology, mineral and whole-rock chemistry of the largest ultramafic body located within the Mesoarchaean Akia terrane, known as the Ulamertoq ultramafic body, to elucidate the poly-metamorphic and metasomatic events that overprinted the protolith. Pronounced lithologic zoning from hydrous mineral-rich layers to orthopyroxene-rich ultramafic rocks at the boundaries between ultramafic rocks and the granitoid country rocks was formed locally by metasomatic reactions related to the granitoids. The main body of ultramafic rocks, far from the contacts, can be classified into four types based on mineral assemblage and chemistry. The fine-grained orthopyroxene aggregates and large poikilitic orthopyroxenes have low Cr2O3 and CaO contents, suggesting a secondary origin. Trace element compositions of orthopyroxene and/or amphibole in the main ultramafic rocks indicate that at least three types of metasomatic agents were required to form these minerals and the associated whole-rock chemical variations within the ultramafic body. Variations represent differences in the proportions of metasomatic orthopyroxene and/or amphibole and phlogopite added to a dunitic protolith. The main body of Ulamertoq ultramafics experienced metasomatism under granulite-facies. Retrograde cooling occurred, to 650°C–850°C and &lt;1.8 GPa prior to local metasomatism via country-rock reaction. The presence of titanian clinohumite and its associated mineral assemblage in the least-metasomatised dunites suggest the possibility that the main ultramafic rocks went through a hydration/dehydration process at ~800°C–900°C and &lt;2 GPa prior to metasomatic modification. This study demonstrates that it is important to consider the effects of multi-stage metasomatism and metamorphism in order to elucidate the origin of the Archaean ultramafic rocks in Greenland and elsewhere.
INVESTIGATION OF SHORT-TERM CHEMICAL CHANGE IN STABLE RUTHENIUM ADDED TO RAINWATER THROUGH X-RAY ABSORPTION FINE STRUCTURE ANALYSIS
Authors: Unno Yusuke , Takeda Akira , Takaku Yuichi 
Published: February 2022

Abstract
Radioactive ruthenium may be accidentally released from spent nuclear fuel reprocessing plants to the surrounding environment. To obtain basic information regarding the effect of radioactive ruthenium on the environment, we investigated changes in the source form of stable ruthenium added to rainwater through X-ray absorption fine structure analysis. Ruthenium tetroxide (RuO4), ruthenium dioxide (RuO2), ruthenium nitrosyl nitrate (Ru(NO)(NO3)3) and ruthenium chloride (RuCl3) were employed as test sources. The Ru K-edge X-ray absorption near edge structure spectra acquired immediately after addition to ultrapure water differed among various sources. With RuO4 addition, a black deposit was observed 1 d after addition, and the spectrum was similar to that of RuO2. For RuO2, Ru(NO)(NO3)3 and RuCl3, no variances were observed between the spectra acquired immediately after addition and 1 d after addition. These results indicate that the chemical forms of RuO2, Ru(NO)(NO3)3 and RuCl3 did not change within a 1-d span in rainwater.
Childhood Trauma May Increase Risk of Psychosis and Mood Disorder in Genetically High-risk Children and Adolescents by Enhancing the Accumulation of Risk Indicators
Authors: Berthelot Nicolas , Garon-Bissonnette Julia , Jomphe Valérie , Doucet-Beaupré Hélène , Bureau Alexandre , Maziade Michel 
Published: February 2022

AbstractBackgroundGenetically high-risk children carry indicators of brain dysfunctions that adult patients with schizophrenia or bipolar disorder display. The accumulation of risk indicators would have a higher predictive value of a later transition to psychosis or mood disorder than each individual risk indicator. Since more than 50% of adult patients report having been exposed to childhood trauma, we investigated whether exposure to trauma during childhood was associated with the early accumulation of risk indicators in youths at genetic risk.MethodsWe first inspected the characteristics of childhood trauma in 200 young offspring (51% male) born to a parent affected by DSM-IV schizophrenia, bipolar disorder, or major depressive disorder. A subsample of 109 offspring (51% male) had measurements on four risk indicators: cognitive impairments, psychotic-like experiences, nonpsychotic nonmood childhood DSM diagnoses, poor global functioning. Trauma was assessed from direct interviews and reviews of lifetime medical and school records of offspring.ResultsTrauma was present in 86 of the 200 offspring (43%). The relative risk of accumulating risk indicators in offspring exposed to trauma was 3.33 (95% CI 1.50, 7.36), but more pronounced in males (RR = 4.64, 95% CI 1.71, 12.6) than females (RR = 2.01, 95% CI 0.54, 7.58).ConclusionChildhood trauma would be related to the accumulation of developmental precursors of major psychiatric disorders and more so in young boys at high genetic risk. Our findings may provide leads for interventions targeting the early mechanisms underlying the established relation between childhood trauma and adult psychiatric disorders.
The Impact of the COVID-19 Pandemic on Women-Led Businesses
Authors: Torres Jesica , Maduko Franklin , Gaddis Isis , Iacovone Leonardo , Beegle Kathleen 
Published: February 2022

AbstractThe COVID-19 pandemic has struck businesses across the globe with unprecedented impacts. The world economy has been hit hard and firms have experienced a myriad of challenges, but these challenges have been heterogeneous across firms. This paper examines one important dimension of this heterogeneity: the differential effect of the pandemic on women-led and men-led businesses. The paper exploits a unique sample of close to 40,000 mainly formal businesses from 49 countries covering the months between April and September 2020. The findings show that women-led micro-businesses, women-led businesses in the hospitality industry, and women-led businesses in countries more severely affected by the COVID-19 shock were disproportionately hit compared with businesses led by men. At the same time, women-led micro-firms were markedly more likely to report increasing the use of digital platforms, but less likely to invest in software, equipment, or digital solutions. Finally, the findings also show that women-led businesses were less likely to have received some form of public support although they have been hit harder in some domains. In a crisis of the magnitude of the COVID-19 pandemic, evidence tracing the impact of the shock in a timely fashion is desperately needed to help inform the design of policy interventions. This real-time glimpse into women-led businesses fills this need for robust and policy-relevant evidence, and due to the large country coverage of the data, it is possible to identify patterns that extend beyond any one country, region, or sector, but at the cost of some granularity for testing more complex economic theories.
Teaching Medical Students to Teach: A Narrative Review and Literature-Informed Recommendations for Student-as-Teacher Curricula
Authors: Cohen Alexandra , Steinert Yvonne , Ruano Cea Elisa 
Published: February 2022

Purpose
Medical students play an important role as teachers. This narrative review synthesizes the student-as-teacher (SaT) literature that incorporates formal teaching skills training, with the goal of proposing literature-informed recommendations for SaT curriculum development within the context of competency-based medical education.

Method
In January and August 2020, the authors searched Medline and Embase and conducted forward citation searches in Scopus to identify articles from 1985 to 2020 related to teaching medical students to teach. Search terms included “student(s) as teacher(s),” “near-peer teaching,” and “teaching to teach.” The findings from selected articles were summarized and synthesized.

Results
Forty-three articles met the inclusion criteria: 33 papers describing specific SaT curricula, 3 literature reviews, 6 stakeholder surveys, and 1 Delphi study. While students often engage in teaching roles, formal teaching skills training is not always provided. Existing curricula are usually optional, delivered as short courses, and offered to senior students. Course content commonly includes topics related to planning and delivering learning activities and providing feedback. Of the numerous teaching modalities employed, students indicate a preference for interactive and experiential learning. Methods used to assess students’ teaching skills vary; however, direct observation and feedback are valued assessment methods. Program evaluations have shown positive outcomes, including high student satisfaction and enhanced self-confidence as teachers.

Conclusions
Informed by these findings and outcome-based educational frameworks, the authors suggest 4 guiding principles for SaT curricula: (1) teaching skills training should be formally implemented in undergraduate medical education and should, whenever possible, be compulsory; (2) teaching skills should be taught longitudinally and progressively along the undergraduate to postgraduate continuum; (3) curriculum content should be aligned with a school’s preexisting curriculum and local context; and (4) authentic opportunities for students to engage in teaching roles should be included. They also propose recommendations for curriculum content, teaching modalities, and assessment methods.
Principles and test methods of non-contact body thermometry
Authors: Beall Erik B , Askegaard Lars , Berkesch Josh , Adolph Alden C , Hinnerichs Christopher M , Schmidt Matthew 
Published: February 2022

AbstractSignificanceFar infrared (IR) has a long history in thermometry and febrile screening. Concerns have been raised recently over the accuracy of non-contact body thermometry. Clinical testing with febrile individuals constitutes the standard performance assessment. This is challenging to replicate, which may have inadvertently allowed approval of IR systems that are unable to detect fevers. The ability to test performance without relying on febrile participants would have ramifications for public health, especially if this discovered undisclosed differences in accuracy in widely used devices.AimTo identify foundational issues in, demonstrate principles of, and develop test methods for non-contact body thermometry.ApproachWe review foundational literature and identify confounds impeding performance of IR thermography (IRT) and non-contact IR thermometry (NCIT) for febrile screening and demonstrate corrections for their effects, which would otherwise be unacceptable. Almost none of the devices we are aware of compensate for these confounds. We reverse-engineer surface-to-body temperature relations for several FDA-cleared NCITs. We note their similarity to recently reported bias-to-normal behavior in other devices and determine range of body temperatures for which the device would produce a "normal" (non-febrile) output. Finally, we generate predictable elevated face temperatures in healthy subjects and demonstrate this in several devices.ResultsThe surface-to-body relationships for two IRT and one NCIT were linear, while all others exhibited nonlinear bias-to-normal behavior that produce normal temperatures when presented with surface temperatures ranging from hypothermia to moderate-to-severe fever. The test method was used in healthy, non-febrile subjects to generate elevated temperatures corresponding to body temperatures from 97.35F to 102.45F. Three out of five systems had negligible sensitivity.ConclusionsThis demonstrates an alternative evaluation method without the limitations and risks of febrile patients. These results indicate many devices may be unusable for body thermometry and may be providing a false sense of security for public health surveillance.
Variation in colorectal cancer treatment and outcomes in Scotland: real world evidence from national linked administrative data
Authors: Lemmon E , Hanna C , Diernberger K , Hall PS 
Published: February 2022

AbstractBackgroundColorectal cancer (CRC) is the third most common type of cancer in Scotland and the second leading cause of cancer death. Despite improvements in CRC survival over time, Scotland lags behind its UK and European counterparts. Linked administrative datasets can provide a real-world representation of current care as a basis for evaluation of new interventions or policies and to understand variation in care and outcomes. In this study, we aim to provide up to date, population level evidence on CRC treatment and survival in Scotland for patients treated with curative or palliative intent as a basis for the understanding of variation, and to provide data to underpin the evaluation of new treatments.MethodsWe conducted a retrospective analysis of adults with an incident CRC registered on the Scottish Cancer Registry (ICD-10 codes C18-20) between January 2006 and December 2018. Data on patients with incident CRC was linked to hospital inpatient records allowing description of their demographic, diagnostic and treatment characteristics. For a curative cohort (n = 26,204) Cox-Proportional Hazards regression models were used to assess the factors affecting overall survival (OS) and CRC specific survival (CRCS).ResultsOverall, 32,691 (73%) and 12,184 (27%) patients had a diagnosis of colon and rectal cancer respectively. On average, patients with colorectal cancer had two hospital inpatient stays in the five-years pre-diagnosis. Chemotherapy was used in 42% with colon cancer and 30% with colon cancer. Radiotherapy use was 2% and 39% respectively. Five year OS (CRCS) within the curative cohort was 71% (81%) and 75% (82%) for patients with colon and rectal cancer respectively. After accounting for patient and tumour characteristics, survival outcomes demonstrated significant geographical variation.ConclusionsNational linked administrative datasets have the ability to provide real-world representation of the treatments and outcomes for patients with cancer. In a Scottish population of curative patients with CRC, there was significant variation in survival depending on sex and geography.
Partnering with Athletes to Assess Risk of COVID-Related Myocarditis
Authors: Kay Bradley , Feher Attila , Reinhardt Samuel , Cuomo Jason , Arlis-Mayor Stephanie , Lynch Matthew , Johnson Kyle , Kemp Phil , Wagner Henry , Welsh Tyler , Lamy Jerome , Peters Dana , Mojibian Hamid , Young Lawrence H. , Lampert Rachel , McNamara Robert , Baldassarre Lauren A. , Miller Edward J. , Spatz Erica S. 
Published: February 2022

AbstractBackgroundMyocarditis in athletes is a feared complication of SARS-CoV-2, yet guidelines for screening with cardiac magnetic resonance imaging are lacking. Further, stakeholder involvement in the research is rare.HypothesisWe sought to determine the rates of cardiac magnetic resonance imaging evidence of SARS-CoV-2 related myocarditis in student athletes. We hypothesized that rates of myocarditis were lower than initially reported and that including athletes on the research team would enhance participant satisfaction and scientific integrity.MethodsAccordingly, when members of a hockey team were infected with SARS-CoV-2, we invited them and their team physicians to be part of the design of a study assessing the incidence of myocarditis. We performed cardiac magnetic resonance imaging on participating hockey players infected with SARS-CoV-2 and compared them to a healthy lacrosse cohort. Participants were given an optional survey to complete at the end of the study to assess their satisfaction with it.ResultsFour hockey players and two team physicians joined the study team; eight hockey players and four lacrosse players participated in the study. Zero athletes met imaging criteria for myocarditis; delayed enhancement was observed in seven cases and three controls. Athletes supported sharing the findings with the participants. No athletes reported feeling uncomfortable participating, knowing other athletes participated on the research team.ConclusionRates of SARS-CoV-2 myocarditis in young athletes appears to be lower than initially reported. Partnered research is important, especially in populations with more to lose, such as collegiate athletes; future studies should include stakeholders in the study design and execution.Key pointsCardiac MRI findings of myocarditis after COVID infection in young athletes is rare. Subjects of research studies appreciate involvement in the development of the study, and this also builds trust with the research team.