bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism of antibody-specific deglycosylation and immune evasion by Streptococcal IgG-specific endoglycosidases
Authors: Trastoy Beatriz , Du Jonathan , Cifuente Javier , Rudolph Lorena , García-Alija Mikel , Klontz Erik , Deredge Daniel , Sultana Nazneen , Huynh Chau , Flowers Maria , Li Chao , Sastre Diego , Wang Lai-Xi , Corzana Francisco , Mallagaray Alvaro , Sundberg Eric J. , Guerin Marcelo E. 
Published: June 2022

Abstract
Bacterial pathogens have evolved diverse and intricate mechanisms to evade the human immune system, including the production of immunomodulatory enzymes. Streptococcus pyogenes secretes two multi-modular endo-β-N-acetylglucosaminidases, EndoS and EndoS2, that specifically deglycosylate the conserved N-linked glycan at Asn297 on IgG Fc, disabling antibody-mediated effector functions. Amongst thousands of known carbohydrate-active enzymes, EndoS and EndoS2 represent just a handful of enzymes that are specific to the protein portion of the glycoprotein substrate, not just the glycan component. Here, we present the cryoEM structure of EndoS in complex with the IgG1 Fc fragment. In combination with small-angle X-ray scattering, alanine scanning mutagenesis, hydrolytic activity measurements, enzyme kinetics, nuclear magnetic resonance and molecular dynamics analysis, we establish the mechanisms of recognition and specific deglycosylation of IgG antibodies by EndoS and EndoS2. Our results provide a rational basis from which to engineer novel enzymes with antibody and glycan selectivity for clinical and biotechnological applications.
Proposed process for extracorporeal monitoring and manipulation of circulating molecules in blood for critically ill patients.
Authors: Chander Bal 
Published: June 2022

Abstract
All organs are interdependent and communicate with each other via molecules and ions circulating in the blood. Most of the time the relationship between the biomolecules is non linear and dynamics and therefore it is important to carry out real time estimation of multiple circulating molecules and ions in blood. Existing sensors make it possible to monitor only a few molecules primarily directed at glucose levels. All such sensors face the problem of interference by non target molecules as well as the intervening tissue. The only solution appears to be an extracorporeal circuit with multiple embedded sensors for real time monitoring of multiple molecules. The circuit also serves the function of reducing the complexity of blood by fractionation wherein cellular components are separated from plasma and latter is further separated into fractions according to the need. Fractionation minimizes interference by non target molecules. It is also important to be able to reduce the high levels of circulating molecules in a controlled and selective manner without using any drugs. As a result of reduced complexity of plasma and real time estimation, such manipulation can be effected via magnetized nano particles designed to capture via chemical reaction a single type of molecule. The proposed process describes the design with underlying rationale along with advantages offered and limitations.
Regorafenib is suitable for advanced colorectal cancer patients who have previously received trifluridine/tipiracil plus bevacizumab
Authors: Matsumoto Toshihiko , Ikoma Tatsuki , Yamamura Shogo , Miura Kou , Tsuduki Takao , Watanabe Takanori , Nagai Hiroki , Takatani Masahiro , Yasui Hisateru 
Published: June 2022

Abstract
Background:
Regorafenib is a standard salvage line therapy used for advanced colorectal cancer (CRC). Recently, FTD/TPI plus bevacizumab also showed promising efficacy as a salvage line therapy for advanced CRC. However, the efficacy and safety of regorafenib for patients with advanced CRC who have previously received trifluridine/tipiracil (TFTD) plus bevacizumab is unclear.
Results:
We retrospectively collected clinicopathologic data from patients with advanced CRC who received regorafenib after TFTD plus bevacizumab in multiple institutions between April 2017 and June 2020.Thirty-four advanced CRC patients who received regorafenib were analyzed. The median age was 66.5 (range 43 − 81 years), 11 patients were male, and all had an ECOG performance status(PS) of 0 or 1. Twenty-two patients had left-sided tumors, 18 patients had RAS mutants, and 1 patient had a BRAF V600E mutation. The response rate was 0%, and the disease control rate was 31%. The median progression-free survival was 70 days (95% CI: 56 − 91), and the overall survival was 233 days (95% CI: 188 − 324). Treatment was discontinued in 32 patients, and 28 (82%) discontinued treatment due to progressive disease. The major grade 3 and4 toxicities were proteinurea (29%), hypertension (26%), hand-foot syndrome(15%), and platelet decrease (6%).
Conclusion:
Regorafenib after TFTD plus bevacizumab showed efficacy similar to that of the previous study, and no new adverse events were observed.
Adherence to Healthy Eating Index-2015 and Obesity among Iranian Adults: Yazd Health Study-TAMYZ and Shahedieh Cohort Study
Authors: Sarkhosh-Khorasani Sahar , Nadjarzadeh Azadeh , Mozaffari-Khosravi Hassan , Mirzaei Masoud , Hosseinzadeh Mahdieh 
Published: June 2022

Abstract
Objective
Data demonstrated a relationship between obesity and increasing the risk of mortality and morbidity of chronic diseases. Inconsistent data regarding the association between adherence of Healthy Eating Index-2015 (HEI-2015) and obesity is identified. Thus, this study intends to address this relationship among a large sample of both urban and suburb areas of Iranian adults.
Methods
This cross-sectional study was performed on data of 10693 participants including Shahedieh cohort study (n = 3943) and TAghzieh Mardom-e-Yazd (TAMYZ) which was conducted on Yazd Health Study participants, (n = 6750) living in urban and suburb areas. Dietary intake was assessed by using a validated Food Frequency Questionnaire (FFQ). In all participants, anthropometric indices including body mass index were measured. Multivariate logistic regression analysis was utilized to assess the relationship of HEI-2015 with obesity.
Results
No significant association was found between adherence to the HEI-2015 diet and odds of general and central obesity.
Conclusion
Our findings showed adherence to HEI-2015 might have no protective effect on odds of general obesity. Also, in suburb region and whole population of both urban and suburb regions greater compliance of men to HEI-2015 diet might have a positive effect on odds of central obesity. Moreover, in suburb area participants in the highest quintile significantly were smoker and had higher level of SES. Finally, the casual effect of HEI-2015 on obesity needs to be investigated further in prospective studies and among different populations.
El papel de las alianzas público-privadas financiadas por la GIZ en la reducción de la violencia de género en América Latina
Authors: Pintor Pirzkall Heike Clara 
Published: June 2022

La violencia de género es una pandemia que afecta, sin excepción, a todo el continente latinoamericano. Las autoridades nacionales han realizado importantes avances legislativos con relación a la violencia de género, pero a nivel práctico el alcance ha sido limitado y las cifras siguen creciendo. La Sociedad Alemana para la Cooperación Internacional (GIZ) ha impulsado una serie de proyectos con empresas y gobiernos para reducir la violencia de género tanto dentro como fuera del entorno empresarial, y cumplir así con la Estrategia de Género desarrollada por la GIZ en el año 2013. Este artículo es la segunda parte de un estudio iniciado en el año 2019 en relación con el Programa Regional “Combatir la Violencia contra las Mujeres en Latinoamérica” – ComVoMujer (2009-2018) gestionado por la GIZ en colaboración con 270 empresas latinoamericanas. La primera parte del estudio ha sido publicada en la editorial Universidad de Salamanca en el año 2020 y se centra en las empresas como zonas de paz. En esta segunda parte se pretende valorar el impacto del proyecto ComVoMujer II (2014-2018) que se implementó en Perú, Ecuador, Bolivia y Paraguay. Esta valoración se realiza por medio una serie de indicadores que permiten hacer un balance de las aportaciones hechas por dicho proyecto teniendo en cuenta el número de actividades realizadas, el número de personas participantes, el impacto de las acciones en la sociedad y el papel que han jugado las alianzas público-privadas entre las empresas privadas y las instituciones gubernamentales en la disminución de la violencia de género, con el fin de averiguar si estas acciones deberían incluirse en una estrategia regional a largo plazo.

Palabras clave: violencia de género; América del Sur; proyecto ComVoMujer II; Alianzas público-privadas; Cooperación alemana al desarrollo.
Strategic Analysis of the Construction Industry in Kyrgyzstan
Authors: Kocherbaeva Ainura , Kalpaeva Zarina 
Published: June 2022

This article discusses company strategies. In strategic management, a strategy is a long-term qualitative direction that the company develops to achieve its goals. Basically, a strategy is what companies should do to achieve their goals in an unstable and competitive environment. The strategic analysis assesses the current and future state of the organization and determines its strategic choices. The present research featured the construction industry in the Kyrgyz Republic. It relied on Professor V.L. Kvint’s theory of strategizing in the economic system. In modern conditions, construction business requires a proper development strategy to adopt new innovative solutions that allow construction companies to reach a new level of quality. Construction companies can develop strategies based on the specific characteristics of their products. Business requires investments. On the one hand, the investment complex is a self-sufficient reproductive system. On the other hand, it is an integral part of a much larger system, i.e., the state. The research revealed no single methodology
for selecting and implementing particular types of strategic analysis because each construction company is unique. Managers must have a clear vision of all aspects of the long-term development. Strategies are often
limited to option-specific commitments, so decisions need to be considered and evaluated very carefully.
A novel approach for estimating the capacity of ungauged small reservoirs using remote sensing and DEM
Authors: Cao Shengle , You Ruifeng , Li Xinying , Jia Jingjing , Wang Jun , Liu Yang 
Published: June 2022

Abstract
The estimation of small reservoir capacity is of great significance for water resources management. However, many widely distributed small reservoirs lack the capacity information because of the high costs of field measurements. This study proposed a novel approach to estimate the small reservoir capacity in the hilly area by using remote sensing and Digital Elevation Model (DEM). The basic idea of this approach is to explore the relationship between influential factors (i.e., topographic and geomorphic parameters) and measured reservoirs’ capacity to establish a machine learning model based on particle swarm optimization–extreme learning machine (PSO–ELM) to estimate the capacity. The Mihe River basin in northern China is selected as a case study, 111 measured reservoirs, and six optional influential factors are selected to develop and test this model. The results show that the five influential factors (i.e., the area of sub-catchment, the water surface area, the longest flow path of sub-catchment, the average slope of sub-catchment, and the average slope of buffer area) are the optimal combination with the lowest difference between the measured and the estimated reservoir capacities. The results demonstrate that the proposed approach is a robust tool for estimating the capacity of small reservoirs in the hilly area.
Understanding the impact of changes in land-use land-cover and rainfall patterns on soil erosion rates using the RUSLE model and GIS techniques: A study on the Nagavali River basin
Authors: Yaswanth Kottali , Kona Meghana , Andra Sai Kumar , Rathinasamy Maheswaran 
Published: June 2022

Abstract
Soil erosion is the most common type of land degradation, and it has become a global environmental issue that reduces soil productivity and water quality and is accelerated by human-induced activities. The Revised Universal Soil Loss Equation (RUSLE) model, which includes factors like rainfall, soil, land-cover, cultivation practices, and slope, was incorporated into the Geographic Information System (GIS) database to estimate average annual soil erosion rates in the Nagavali River basin (NRB) during 1990 and 2020, and this study also focuses on analyzing the impact of the land-use and land-cover (LULC) change, as well as climate variability on the annual average soil erosion rates in the NRB. Results indicate that the average annual soil erosion rate in the NRB ranged from 0 to 2364.46 t/ha/yr in the year 1990 was increased to 7857.21 t/ha/yr by the year 2020 as a result of an increase in rainfall and LULC changes over the study period. Based on the spatial distribution of soil erosion risk classes, it was identified that the area under the very severe erosion class increased drastically from 4.34 to 13.97%, while the area under the very slight erosion class got decreased between the years 1990 and 2020. Accordingly, a correlation analysis was carried out to determine the spatial correlation between the soil erosion pattern and changes in driver variables such as rainfall and land-use classes. These findings can be used to make well-informed decisions at the local and regional levels for the sake of soil conservation.
How much spatial and temporal variation in groundwater microbiology can occur following open dumping of municipal solid waste?
Authors: Naresh Kumar R. , Verma Mohini , Iqbal Jawed , Chakraborty Shubhrasekhar 
Published: June 2022

Abstract
The effects of open dumping of municipal solid waste on local groundwater microbiology were assessed in Ranchi, India. Both the spatial and temporal variations of groundwater microbiology were studied. Groundwater was analyzed to determine heterotrophic plate count (HPC), total coliforms (TC) and faecal coliforms (FC). Highest HPC was 4.5 × 104 CFU/mL and the highest total coliform count was 3.7 × 104 CFU/mL. Faecal coliforms were detected in most of the groundwater samples. The highest faecal coliform count of 2.1 × 104 CFU/mL was from a hand pump located adjacent to the dumping site. Spatial variations revealed dispersion of bacterial contamination up to 5 km from the periphery of the municipal solid waste dump. The level of bacterial contamination decreased with an increase in distance from the municipal solid waste dump. The maximum bacterial count was detected in the monsoon whereas the lowest count was found in summer. Molecular characterization of morphologically similar faecal coliform colonies indicated the presence of Klebsiella pneumoniae (Klebsiella ssnkbit, KU647674) in one of the groundwater samples that showed maximum faecal coliform count. Results of this study indicated that groundwater in the area is highly contaminated with a bacterial load which can be a major health risk.
THE EFFECT OF VARIATION OF ZEOLITE AS ADSORBENT MEDIUM AND ADSOPTION PRESSURE TOWARD THE QUALITY OF OXYGEN PRODUCED FROM PRESSURE SWING ADSORPTION (PSA)
Authors: Satria Dhimas , Kurniawan Teguh , Salman Nidya Jullanar 
Published: June 2022

One of the most important element in human life is oxygen. To produce good quality of oxygen, a purification process is needed. Pressure Swing Adsorption with the adsobent of zeolite is the most comon method used for oxygen purification. This study aimed to find out media variation of of zeolite as adsorbent medium and adsoption pressure toward oxygen quality produced from PSA. Variation of adsrobent medium used were 13X zeolite and combination of 13X zeolite and Bayah zeolite (zeolite 13X+ZAB). The study result shows that the use of 13X zeolite and 13X+ZAB zeolite can significanly increase the quality of oxygen in PSA compare to without using zeolite. The use of 13X zeolite produce higher quality of oxygen, this is because 13X zeolit is a zeolite which has high ability to adsorb nitrogen and 13X zeolite has high surface area. The higher the adsorbent surface area the more adsorbat was adsrobed so that 13X zeolite will adsorb niotrogen. Meanwhile the addition of Bayah natural zeolite into 13x zeolite (13X zeolite +ZAB) is to overcome the problem of the vapor releasing which can hinder the performance of PSA in certain period of time, so that it is needed one media of adsorbent of natural zeolite which can adsorb liquid produced from this PSA. The effect of pressure variation toward oxigen quality produced from PSA is that the higher the pressure applied the faster the process of lowering the quality of oxygen would be, this is because the adsorben was more saturated. The pressure highly affect the adsorbability occur in PSA colomn.
Dieta y nicho de alimentación del coyote, zorra gris, mapache y jaguarundi en un bosque tropical caducifolio de la costa sur del estado de Jalisco, México
Authors: Guerrero Sergio , Badii Mohammad H. , Zalapa Silvia S. , Flores Adriana E. 
Published: June 2022

Se analizó la dieta, amplitud y sobreposición de nicho de alimentación para el coyote (Canis latrans), zorra gris (Urocyon cinereaoargenteus), mapache (Procyon lotor) y jaguarundi (Herpailurus yagouaroundi) en una zona de bosque tropical caducifolio de la costa sur de Jalisco, México. Para ello se recolectaron 430 excrementos de las cuatro especies: coyote (118), zorra gris (78), mapache (198) jaguarundi (36), fueron analizados y los contenidos determinados. Los resultados son reportados en frecuencia relativa de ocurrencia (FRO). Para el análisis de amplitud de nicho se aplicó el índice de Levins y el de Renkonen paradeterminar sobreposición de nicho. Los resultados mostraron que el material vegetal fue el elemento de mayor FRO para coyote (36.76%), zorra gris (38.16%) y mapache (47.62%), mientras que los mamíferos lo fueron para jaguarundi (43.37%). Otros grupos como insectos, aves, crustáceos y peces complementan la dieta de estas especies en diferentes proporciones; cambios en la frecuencia de esos grupos en cada especie son reportados entre la estación húmeda y seca. El valor con mayor amplitud de nicho lo presentó la zorra gris (15.32) y el coyote (14.95) y el menor el mapache (11.16) y el jaguarundi (4.46), aunque estos valores se ven modificados al analizar los datos por estación del año (seca-húmeda). Respecto a la sobreposición del nicho de alimentación, el mayor fue para coyote-zorra gris (57.18%) y el menor fue para mapache y jaguarundi (19.95%). Estacionalmente, el coyote y el jaguarundi registraron el mayor valor en la sobreposición de nicho, tanto en la estación seca (58.26%) como en la húmeda (49.48%).
New record of the culpeo Lycalopex culpaeus (Mammalia: Canidae) in southwestern Colombia with remarks on its distribution in the country
Authors: Ramírez-Chaves Héctor E. , Chaves-Salazar Juan Manuel , Mendoza-Escobar Richard Hernán 
Published: June 2022

We present a new record of the culpeo (Lycalopex culpaeus) from southwestern Colombia, and discuss the previous records and localities of the species in the country. The species is known from three confirmed localities; two of them are represented by voucher specimens and the third is presented here based on a photography obtained on 2011. All confirmed localities are located in the Nudo de Los Pastos, Department of Nariño, southwestern Colombia. Other localities and records of the species in literature are in need of more evidence that corroborate the presence of the species in these areas, while the records from the Central Cordillera in the Departments of Valle del Cauca, Tolima, and from the Western Cordillera in the Department of Cauca are considered erroneous.
Anticonvulsant Activity of Lacidipine against Mes- and Ptz-Induced Seizures
Authors: Pushpa V H  , Jayanthi M. K  , Mallikarjun S  , Ramith Ramu  
Published: June 2022

Epilepsy is one of the challenging medical conditions affecting neonates, children and elderly. It has affected 1710 to 9780/million populations within India. Although the report of this condition dates to the prehistoric era, drugs for its treatment are not 100% effective and more often lead to toxic effects. Therefore, a potent drug or a drug adjunct is the need-of-the-hour for providing effective control for this neurological condition. The aim of the present study is to evaluate lacidipine, a calcium channel blocker as a protective agent in seizures induced by standard models of epilepsy, namely maximal electroshock seizure model (MES) and Pentylenetetrazole (PTZ). Standard methods for the induction of seizures such as MES and PTZ were performed on male Wistar albino mice. The mice were then treated with vehicle/standard drug (sodium valproate)/ lacidipine. Further, the standard parameters of recovery duration were evaluated. The study revealed that lacidipine at a dose of 3mg/kg body weight significantly reduced the duration of hind limb tonic extension and postictal depression in MES. PTZ-induced seizures showed significantly reduced mean duration of hind limb tonic flexion, hind limb tonic extension, clonus and postictal depression. The findings from the present study suggest that lacidipine has potent anticonvulsant ability in better protection against MES-induced seizures over that of the PTZ-induced one.
Use of a novel helical fan beam imaging system for computed tomography of the head and neck in sedated standing horses: 120 cases (2019–2020)
Authors: Brounts Sabrina H. , Henry Travis , Lund Jane R. , Whitton R. Chris , Ergun David L. , Muir Peter 
Published: June 2022

Abstract

OBJECTIVE
To evaluate the diagnostic capabilities of a novel helical fan beam CT system used for imaging of horses with clinical problems of the head and neck.

ANIMALS
120 horses.

PROCEDURES
Medical records were reviewed of horses presented for CT of the head or neck at 2 university hospitals. The following data were recorded: age, sex, breed, presenting complaint, sedation used for imaging, scanning time, procedure time, other diagnostic imaging methods performed, imaging diagnosis, clinical diagnosis, and complications during imaging.

RESULTS
Quarter Horses and Warmbloods were the most common breeds, and the most common complaint was nasal discharge. The head (101/120 [84%] horses) was scanned most frequently, and the most common diagnoses were primary dental disease and a space-occupying lesion of the paranasal sinuses. Nuchal bursitis was the most common imaging diagnosis in the neck region. Procedure time ranged from 20 to 45 minutes with a scanning time of 30 to 40 seconds. No complications with horse sedation occurred, and horses tolerated scanning well. An imaging diagnosis was more frequently achieved with CT (109/120 [91%] horses) than with planar digital radiography (23/61 [38%] horses).

CLINICAL RELEVANCE
The helical fan beam CT system had fast scanning times and could be used safely for routine imaging of the teeth and sinuses in horses. The caudal extent of scanning in the neck region was limited by the shape of the horse’s neck and thorax.