bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation the effect of Iron oxide nanoparticles functionalized by glucose and conjugated with Coumarin (Fe3O4@Glu-coumarin NPs) on expression of CASP8, CASP9, p53, mTOR1, and MAPK1 genes in liver cancer cell line
Authors: Shokrollahi Faezeh , Salehzadeh Ali , Kafilzadeh Farshid , Zaefizadeh Mohammad 
Published: December 2022

Abstract

Background: Due to the high morbidity and mortality of liver cancer, many researchers are seeking novel anticancer formulations. Complex iron oxide containing compounds could be used for site directed drug delivery using a magnetic force. Due to the anticancer property of coumarin, this study aimed to study the effect of iron oxide nanoparticles functionalized by glucose and conjugated with Coumarin (Fe3O4@Glu-coumarin NPs) on the expression of cell cycle and apoptosis regulating genes in a liver cancer cell line.
Methods: Fe3O4@Glu-coumarin NPs were synthesized and their physicochemical properties were evaluated by FT-IR, EDS mapping, and electron microscope imaging. Anti-proliferative activity of the NPs on HepG2 cells was studied by MTT assay. The effect of Fe3O4@Glu-coumarin NPs on the expression of CASP8, CASP9, p53, mTOR1, and MAPK1 genes was investigated by quantitative PCR.
Results: The synthesized NPs were spherical, with a moderate level of aggregation and in a size range of 26-46 nm. FT-IR and EDS mapping confirmed the proper synthesis and purity of the synthesized NPs. According to the MTT assay, Fe3O4@Glu-coumarin NPs showed a considerable anti-proliferative effect on liver cancer cells, and the 50% inhibitory concentration was determined 120µg/mL. Quantitative PCR assay showed that the NPs significantly increased the expression of CASP8, p53, and MAPK1 genes by 1.94, 4.87, and 3.87 folds, respectively, while the mTOR1 gene was reduced by -6.33 folds. The expression of the CASP9 had an insignificant reduction by 0.06 folds.
Conclusions: Our results showed that Fe3O4@Glu-coumarin NPs could induce cell apoptosis by interfering with the expression of the cell regulatory genes.
The essential roles of FXR in diet and age influenced metabolic changes and liver disease development: a multi-omics study
Authors: Yang Guiyan , Jena Prasant K. , Hu Ying , Sheng Lili , Chen Shin-Yu , Slupsky Carolyn M. , Davis Ryan , Tepper Clifford G. , Wan Yu-Jui Yvonne 
Published: December 2022

Abstract
Background
Aging and diet are risks for metabolic diseases. Bile acid receptor farnesoid X receptor (FXR) knockout (KO) mice develop metabolic liver diseases that progress into cancer as they age, which is accelerated by Western diet (WD) intake. The current study uncovers the molecular signatures for diet and age-linked metabolic liver disease development in an FXR-dependent manner.
Methods
Wild-type (WT) and FXR KO male mice, either on a healthy control diet (CD) or a WD, were euthanized at the ages of 5-, 10-, or 15-months. Hepatic transcriptomics, liver, serum, and urine metabolomics as well as microbiota were profiled.
Results
WD intake facilitated hepatic aging in WT mice. In an FXR-dependent manner, increased inflammation and reduced oxidative phosphorylation were the primary pathways affected by WD and aging. FXR has a role in modulating inflammation and B cell-mediated humoral immunity which was enhanced by aging. Moreover, FXR dictated neuron differentiation, muscle contraction, and cytoskeleton organization in addition to metabolism. There were 654 transcripts commonly altered by diets, ages, and FXR KO, and 76 of them were differentially expressed in human hepatocellular carcinoma (HCC) and healthy livers. Urine metabolites differentiated dietary effects in both genotypes, and serum metabolites clearly separated ages irrespective of diets. Aging and FXR KO commonly affected amino acid metabolism and TCA cycle. Moreover, FXR is essential for colonization of age-related gut microbes. Integrated analyses uncovered metabolites and bacteria linked with hepatic transcripts affected by WD intake, aging, and FXR KO as well as related to HCC patient survival.
Conclusion
FXR is target to prevent diet or age-associated metabolic disease. The uncovered metabolites and microbes can be diagnostic markers for metabolic disease.
Effectiveness of a Biofeedback Intervention Targeting Mental and Physical Health among College Students through Speech and Physiology as Biomarkers using Machine Learning: A Randomized Controlled Trial
Authors: Wang Lifei , Liu Rongxun , Wang Yang , Xu Xiao , Zhang Ran , Wei Yange , Zhu Rongxin , Zhang Xizhe , Wang Fei 
Published: December 2022

Abstract
Background

Biofeedback therapy is mainly based on the analysis of physiological features to improve an individual’s affective state. There are insufficient objective indicators to assess symptom improvement after biofeedback. In addition to psychological and physiological features, speech features can precisely convey information about emotions. The use of speech features can improve the objectivity of psychiatric assessments. Therefore, biofeedback based on subjective symptom scales, objective speech, and physiological features to evaluate efficacy provides a new approach for early screening and treatment of emotional problems in college students.

Methods

A four-week, randomized, controlled, parallel biofeedback therapy study was conducted with college students with symptoms of anxiety or depression. Speech samples, physiological samples, and clinical symptoms were collected at baseline and at the end of treatment, and the extracted speech features and physiological features were used for between-group comparisons and correlation analyses between the biofeedback and wait-list groups. Based on the speech features with differences between the biofeedback intervention and wait-list groups, an artificial neural network was used to predict the therapeutic effect and response after biofeedback therapy.

Results

Through biofeedback therapy, improvements in depression (p = 0.001), anxiety (P = 0.001), insomnia(P = 0.013), and stress(P = 0.004) severity were observed in college-going students (n = 52). The speech and physiological features in the biofeedback group also changed significantly compared to the waitlist group (n = 52) and were related to the change in symptoms. The energy parameters and Mel-Frequency Cepstral Coefficients (MFCC) of speech features can predict whether biofeedback intervention effectively improves anxiety and insomnia symptoms and treatment response. The accuracy of the classification model built using the artificial neural network (ANN) for treatment response and non-response was approximately 60%.

Conclusions

The results of this study provide valuable information about biofeedback in improving the mental health of college-going students. The study identified speech features, such as the energy parameters, and MFCC as more accurate and objective indicators for tracking biofeedback therapy response and predicting efficacy.

Trial Registration

ClinicalTrials.gov ChiCTR2100045542
An optimal AOWC based on XGM in dual WTWSOAs with co- and counter-propagation schemes for NRZ, RZ, CSRZ and Duobinary signals
Authors: name Parashuram no family , Kumar Chakresh 
Published: December 2022

Abstract
In this article, we propose and demonstrate an optimal approach of all-optical wavelength conversion (AOWC) based on cross gain modulation (XGM) exploiting dual wideband travelling wave semiconductor optical amplifier (WTWSOA) for both counter- and co-propagating schemes. In counter propagating scheme, dual SOAs are in feedback loop whereas in co-propagating scheme dual SOAs are cascaded employing amplified synchronous emission (ASE) noise source. Input data signal of 193.4 THz is optically modulated employing different transmitters such as NRZ, RZ, CSRZ and Duobinary, for conversion at 128 Gbps. Good performance is achieved for ultrawideband wavelength conversion using both propagating schemes. The system performance is analyzed using carrier density curve as a function of distance in WTWSOA, bit error rate (BER), quality factor (Q) and extinction ratio (ER) of the eye diagram. Maximum Q factor of 14.06 at minimum BER of 2.38 × 10−45 is obtained for counter-propagating scheme using NRZ optical transmitter at -5 dBm input signal power. Maximum Q factor of 17.84 at minimum BER of 1 × 10−71 is obtained for co-propagating scheme using CSRZ optical transmitter at 0 dBm input signal power. Power penalty free wavelength conversion is realized due to high ER (> 15 dB) at optimized input conditions.
Influence of holmium doping and oxygen nonstoichiometry on the transport properties of perovskite-type Ca0.6−x Sr0.4HoxMnO3−δ
Authors: Konstantinova Ekaterina I. , Ryzhkov Maxim A. , Leonidova Olga N. , Litvinov Vladimir A. , Leonidov Ilia A. 
Published: December 2022

Abstract
The Ca0.6−xSr0.4HoxMnO3−δ (x = 0.05, 0.10 and 0.15) manganites featured an orthorhombic crystal structure (space group Pbnm) have been produced via citrate-nitrate route in air. The electron donor holmium (Ho3+) and oxygen nonstoichiometry strongly affect on electrical conductivity (σ), Seebeck coefficient (S), thermal conductivity (κ) and thermal expansion. The increase in the absolute values of S with temperature and the decline of σ above 500 K are explained by the decrease in the concentration of Mn3+ ions due to their disproportionation into Mn2+ and Mn4+ ions. The equilibrium constant of disproportionation, concentration and mobility of charge carriers as a function of temperature are obtained from the dependencies S(T) and σ(T). The formation of Mn3+ ions as a result of partial removal of oxygen from the manganites has a strong influence their properties above 1000 K. Temperature activated character of mobility and the values of S are consistent with the adiabatic small polaron transport mechanism in Ca0.6−xSr0.4HoxMnO3−δ.
Electrospherization of genistein@DNA core-shell nanospheres as a drug delivery system
Authors: Elsherbeny Aya A. , Motawea Amira , Tawfik Ashraf M. , Gabr Mohamed M. , elmezayyen ayman 
Published: December 2022

Abstract
The practical application of genistein (GEN) as a drug system is hindered by its low aqueous solubility and poor oral bioavailability. Encapsulation of poor water-soluble drug was considered as one of the widely used approaches to overcome such of these obstacles. The primary goal of this research was to in situ encapsulate the hydrophobic GEN during the electrosynthesis of DNA nanospheres as a delivery system (Es GEN@DNA) with appropriate drug release properties. The prepared Es GEN@DNA nanospheres were characterized using UV-visible spectroscopy, X-ray diffraction analysis (XRD), transmission electron microscope (TEM), zeta potential and stability test. The results revealed that GEN was successfully encapsulate in situ during the DNA electrospherization (Es GEN@DNA) as core shell like structure (Core: GEN and Shell: DNA) with a wonderful stability against time. Furthermore, the drug encapsulation % was studied. In addition, the drug release efficiency of Es GEN@DNA was recorded and theoretically visualized to understand the mechanism and kinetics of GEN drug release. %Encapsulation of GEN within DNA nanospheres was found to be 89.62%. Es GEN@DNA release profile explored that the well entrapped GEN within the DNA nanospheres could be a promising for sustained drug release. Besides, we overcome the dilemma of using a fractal or fractional kinetics model by introducing a general fractional kinetic equation that involves a time-dependent rate coefficient, which introduced that the solution of the fractional kinetic model is capable of fitting the release data profiles of free GEN and Es GEN@DNA.
Finite Element Analysis of Bulging Effect of Aluminium Solid Cylinder During Cold Upsetting
Authors: Bhumarker Amit , Singh Abhishek 
Published: December 2022

Abstract
Friction plays an important role in metal working and manufacturing processes because of the relative motion and forces that are always present on tools, dies, and work pieces. Friction, however, is not directly measurable, nor is it really an independent parameter. Yet in many metal-forming processes, the effect of friction is as important as that of measurable independent parameters. The presence of friction increases the work load and lost the energy. Several methods were employed for calculate the friction but assumed that there is no barreling (or bulging) effect during forging and the calibration curve are developed based on this but the bulging effect showed significant effect on friction factor develop during deformation.
In this study experimental results on deformation of commercial aluminum are compared with those obtained through FEM modeling. Comparison is made using bulging at different specimen heights, different specimen size, axial deformation, lateral deformation and forging load. Deviation in results obtained through experimental work and FEM modeling are determined.
Systematic Qualitative Proteome-wide Analysis of Lysine Malonylation Profiling in Platycodon grandiflorus
Authors: Yang Qingshan , Xu Shaowei , Jiang Weimin , Meng Fei , Wang Shuting , Sun Zongping , Zha Liangping , Gui Shuangying , Peng Daiyin , Liu Juan , Xing Shihai 
Published: December 2022

Abstract
Background
The modification of lysine malonylation found in recent years can affect biological metabolism and play an important role in life activities. Platycodon grandiflorus, as an economic crop and medicinal plant with a wide range of applications and has broad prospects for development. However, there is no reports on malonylation in the related research of P. grandiflorus. This study qualitatively introduces the malonylation of lysine in P. grandiflorus.
Results
888 lysine malonylation modified proteins in P. grandiflorus were identified, with a total of 1755 modification sites. According to the functional annotation, malonylation proteins were closely related to catalysis, binding and other reactions. Subcellular localization showed that related proteins were enriched in chloroplasts, cytoplasm and nuclei, indicating that this modification could regulate a variety of biological metabolic processes. Motif analysis showed that the enrichment of Alanine (A), Cysteine (C), Glycine (G) and Valine (V) amino acids surrounding malonylated lysine residues. Metabolic pathway and protein-protein interaction network analyses suggested modification is mainly involved in plant photosynthesis. Moreover, malonylated proteins also involved in stress and defense responses.
Conclusion
This study shows that lysine malonylation can affect a variety of biological processes and metabolic pathways, and these contents are reported for the first time in P. grandiflorus, which can provide important information for further research on P. grandiflorus and lysine malonylation.
A pan-cancer analysis of charged ubiquitin-specific protease 14's carcinogenic effects
Authors: Gu Anyun 
Published: December 2022

Abstract
According to earlier research, the IDO1 protein is stabilized by overexpression of the ubiquitin-specific protease 14 (USP14), which increases tryptophan metabolism and T-cell failure. These results emphasize the function of USP14 in suppressing anti-tumor immunity and post-translational regulation in IDO1. USP14, however, does not have a Pan-cancer analysis. Using internet databases and R software, we looked at the possible oncogenic involvement of USP14 in 33 cancers using the TCGA and GEO datasets. We found fibroblast infiltration linked with malignancy in various cancers, including cervical squamous cell carcinoma, colon cancer, glioblastoma multiforme, and head and neck squamous cell carcinoma. The carcinogenic significance of USP14 in many malignancies is rather well understood because of the current pan-cancer investigation that we conducted.
Estrogen receptor alpha mediates 17β-estradiol up-regulates autophagy and alleviates vascular senescence
Authors: Xiang Xiuting , Xie LiangZhen , Lin Jieqi , Pare Rahmawati , Huang Guanshen , Huang Jianming , Wang Yuuan , Song Shicong , Ruan Yunjun 
Published: December 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Intrusive Rumination Predicts Depression among Chinese Adolescents during the COVID-19 Pandemic: the Mediation of Sense of Security and the Moderation of Creativity
Authors: Wang Qing , Yuan Yuming , Niu Jia , Xu Yanhua , Liu Di , Luo Jing , Yue Xiaodong , Shi Baoguo 
Published: December 2022

AbstractBackground Until now, the COVID-19 pandemic is still affecting the study and life of Chinese adolescents. Adolescents in high school were likely to experience intrusive rumination to cope with undesirable changes, which could increase the risk of depression. However, few studies have examined the effects of a sense of security and creativity underlying this relationship. This study aims to explore the relationship between intrusive rumination and depression and further examines the mediating role of the sense of security and the moderating role of the creativity in adolescents. This study could provide detailed guidance for government and media to avoid adolescents’ excessive post event reflection on public health incidents. Methods This study investigated 1,269 high school students (748 girls and 521 boys) using the questionnaire method, including revised Event-Related Rumination Inventory (ERRI), Security Questionnaire (SQ), Runco Ideational Behavior Scale (RIBS), and Patient Health Questionnaire (PHQ-9). Results The results showed that intrusive rumination was positively associated with adolescents' depression, and a sense of security could mediate this relationship. Moreover, a possible cut-off of creativity that serves as a breakpoint below and above that can pinpoint where the association between sense of security and depression in adolescents. In particular, compared to less creative adolescents, highly creative adolescents showed higher indirect effects of intrusive rumination on depression. The association between a sense of security and depression is also more robust for highly creative adolescents. Conclusions These results demonstrate that a sense of security may be a protective factor for depression during the COVID-19 pandemic. There are also differences in adolescents' responses to adverse events with different levels of creativity.
Twinning in hydrous wadsleyite: Symmetry relations, origin, and consequences
Authors: Miyajima Nobuyoshi , Buchen Johannes , Kawazoe Takaaki 
Published: December 2022

AbstractTwins in hydrous wadsleyite were detected by polarized-light microscopy and characterized with transmission electron microscopy techniques, including precession selected area electron diffraction and large-angle convergent beam diffraction. By inspecting diffracted intensities for high-order Laue zones, we found the symmetry of our hydrous wadsleyite samples to be reduced to monoclinic with respect to the orthorhombic symmetry of most anhydrous wadsleyite samples. Twinned domains in hydrous wadsleyite share the (122) plane as a composition plane and are related to each other by a twofold rotation around a twin axis parallel to [212] or by reflection on (122). The twin axis and twin plane in wadsleyite correspond to the &lt;101&gt; directions and the {101} planes of ringwoodite, respectively. The twin operations exchange the c* and the [120]* directions of wadsleyite, both of which correspond to the directions of the cubic a axes in ringwoodite. Based on our analysis of symmetry relations and pseudo-symmetry in wadsleyite, we conclude that the twins formed during crystal growth under quasi-hydrostatic conditions in the presence of a hydrous fluid. Twinning in wadsleyite may affect the physical properties and deformation behavior of wadsleyite as well as the phase transition to ringwoodite in the Earth’s mantle transition zone.
IS THERE A DIFFERENCE IN BALANCE FUNCTIONS BETWEEN BREAST CANCER SURVIVOR WOMEN AND HEALTHY WOMEN?
Authors: ZABİT ÖZDEMİR Ferdiye , İYİGÜN Gözde 
Published: December 2022

Purpose: Breast cancer survivors have various physical problems and balance disorders after mastectomy surgery (BCS). The study aimed to compare the balance functions in BCS women who had a mastectomy with healthy women.
Methods: Sixty-six individuals, BCS women who had a mastectomy (BCS group, n=33) and healthy women (control group, n=33), were included in the study. In this study, subjective balance problems and falling characteristics, static balance (Tandem Romberg Test - TRT) and dynamic balance (Y Balance Test- (YBT) and computer-based dynamic balance platform - Technobody-PK 200 WL) functions of the study and control groups were compared.
Results: The individuals in the BCS group (48.48%) experienced more balance problems than the control group (6.06%), but there was no difference between the two groups in terms of falling characteristics. Static balance (TRT) eyes-closed results were lower in the BCS group than the control group (t=-2.21, p=0.03), but there was no difference between the groups in TRT eyes-open results. There was no difference in any sub-parameter in dynamic balance measurements between the groups.
Conclusion: Subjective balance problems and static balance functions are affected more in BCS than in healthy individuals. It is recommended that balance functions should be evaluated and followed up with appropriate treatment methods in BCS women who had a mastectomy.
Remunicipalisation of water services in Europe. Comparative study of the Neapolitan and Parisian cases
Authors: Turri Vanessa Mascia 
Published: December 2022

Abstract
After the politicisation of urban water services governance, over a hundred European cities have implemented water services remunicipalisation reforms inspired by the theory of water management as a common. This paper aims to answer questions about the contextual variables that impact the water utility governance processes and the factors that determine the policy-makers' choices. In particular, the work reconstructs the water remunicipalisation reforms of Paris (2009) and Naples (2011) with the Process-Tracing method, compares them, evaluates their outcomes and formulates previsions about their continuance. We base the analysis on qualitative data collected by documentary investigations and 27 in-depth interviews with protagonists of the remunicipalisation processes. Despite implementing the same management model, the water remunicipalisation has produced profoundly different governance processes and outcomes. The Parisian remunicipalisation was quick, efficient, almost conflict-free, and produced a slight implementation gap, whereas the Neapolitan one was long, complex, highly conflictual and generated a substantial implementation gap. Contextual variables and actors’ behaviour are essential in explaining water policy reform outcomes. The protagonists of analysed reforms have taken decisions based on opportunities and limits defined by their local contexts and the relational systems in which they are historically embedded.