bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patients with polyclonal hepatocellular carcinoma are at a high risk of early recurrence and have a poor recurrence-free survival period
Authors: Kaibori Masaki , Sakai Kazuko , Matsushima Hideyuki , Kosaka Hisashi , Matsui Kosuke , De Velasco Marco A. , Sekimoto Mitsugu , Nishio Kazuto 
Published: January 2022

Abstract
Background/purpose of the study
Tumor heterogeneity based on copy number variations is associated with the evolution of cancer and its clinical grade. Clonal composition (CC) represents the number of clones based on the distribution of B-allele frequency (BAF) obtained from a genome-wide single nucleotide polymorphism (SNP) array. A higher CC number represents a high degree of heterogeneity. We hypothesized and evaluated that the CC number in hepatocellular carcinoma (HCC) tissues might be associated with the clinical outcomes of patients.

Methods
Somatic mutation, whole transcriptome, and CC number based on copy number variations of 36 frozen tissue samples of operably resected HCC tissues were analyzed by targeted deep sequencing, transcriptome analysis, and SNP array.

Results
The samples were classified into the heterogeneous tumors as poly-CC (n = 26) and the homogeneous tumors as mono-CC (n = 8). The patients with poly-CC had a higher rate of early recurrence and a significantly shorter recurrence-free survival period than the mono-CC patients (7.0 months vs. not reached, p = 0.0084). No differences in pathogenic non-synonymous mutations, such as TP53, were observed between the two groups when targeted deep sequencing was applied. A transcriptome analysis showed that cell cycle-related pathways were enriched in the poly-CC tumors, compared to the mono-CC tumors. Poly-CC HCC is highly proliferative and has a high risk of early recurrence.

Conclusion
CC is a possible candidate biomarker for predicting the risk of early postoperative recurrence and warrants further investigation.
The link between the brain volume derived index and the determinants of social performance
Authors: Kokubun Keisuke , Yamakawa Yoshinori , Nemoto Kiyotaka 
Published: January 2022

Abstract
Motivation, defined as the energizing of behavior in pursuit of a goal, is a fundamental element of our interaction with the world and with each other. Furthermore, as it is known that cooperation leads to higher levels of performance than do individual conditions, empathic concern is also crucial to all forms of helping relationships. A growing number of studies indicate that motivation and empathy are associated not only with organizational performance and study achievements, but also with the human brain. However, to date, no definite neuroimaging-derived measures are available to measure motivation and empathy objectively. The current research evaluated the association of motivation and empathy with the whole brain using the gray-matter brain healthcare quotient (GM-BHQ), an MRI-based quotient. Participants were 47 healthy adults. All subjects underwent structural T1-weighted imaging. Motivation levels were evaluated using four motivation scales: Behavioral Activation System (BAS), Self-Monitoring Scale (SMS), Self-Control Scale (SCS), and Behavioral Inhibition System (BIS). Interaction levels, including empathic concern, were evaluated using four subscales of the Interpersonal Reactivity Index (IRI). It was found that the GM-BHQ was most significantly sensitive to the BAS scale (p = 0.002). Furthermore, the GM-BHQ was moderately sensitive to the SMS (p = 0.028) and subscales of the IRI (p = 0.044 for Fantasy and p = 0.036 for Empathic Concern). However, no significant association was found between the GM-BHQ and other variables (BIS and SCS). These results suggest that the GM-BHQ might reflect motivation and empathic concern.
Polycyclic Aromatic Hydrocarbons (PAHs) Sample Preparation and Analysis in Beverages: A Review
Authors: Peng Pau Lian , Lim Lee Hoon 
Published: January 2022

AbstractThe monitoring of food contaminants is of interests to both food regulatory bodies and the consumers. This literature review covers polycyclic aromatic hydrocarbons (PAHs) with regard to their background, sources of exposures, and occurrence in food and environment as well as health hazards. Furthermore, analytical methods focusing on the analysis of PAHs in tea, coffee, milk, and alcoholic samples for the last 16 years are presented. Numerous experimental methods have been developed aiming to obtain better limits of detections (LODs) and percent recoveries as well as to reduce solvent consumption and laborious work. These include information such as the selected PAHs analyzed, food matrix of PAHs, methods of extraction, cleanup procedure, LOD, limits of quantitation (LOQ), and percent recovery. For the analysis of tea, coffee, milk, and alcoholic samples, a majority of the research papers focused on the 16 US Environmental Protection Agency PAHs, while PAH4, PAH8, and methylated PAHs were also of interests. Extraction methods range from the classic Soxhlet extraction and liquid–liquid extraction to newer methods such as QuEChERS, dispersive solid-phase microextraction, and magnetic solid-phase extraction. The cleanup methods involved mainly the use of column chromatography and SPE filled with either silica or Florisil adsorbents. Gas chromatography and liquid chromatography coupled with mass spectrometry or fluorescence detectors are the main analytical instruments used. A majority of the selected combined methods used are able to achieve LODs and percent recoveries in the ranges of 0.01–5 ug/kg and 70–110%, respectively, for the analysis of tea, coffee, milk, and alcoholic samples.
Composition of Sexual Fluids in Cycas revoluta Ovules During Pollination and Fertilization
Authors: von Aderkas Patrick , Little Stefan , Nepi Massimo , Guarnieri Massimo , Antony Madeline , Takaso Tokushiro 
Published: January 2022

AbstractThe composition of fluids that mediate fertilization in cycads is described for the first time. Using tandem mass spectrometry, proteomes of two stages of fluid production, megagametophyte fluid and archegonial chamber fluid production, are compared in Cycas revoluta. These were compared with the proteome of another sexual fluid produced by ovules, the pollination drop proteins. Cycad ovules produce complex liquids immediately prior fertilization. Compared with the pollination drops that mainly had few proteins in classes involved in defense and carbohydrate modification, megagametophyte fluid and archegonial chamber fluid had larger proteomes with many more protein classes, e.g. proteins involved in programmed cell death. Using high-performance liquid chromatography, megagametophyte fluid and archegonial chamber fluid were shown to have elevated concentrations of smaller molecular weight molecules including glucose, pectin and glutamic acid. Compared to megagametophyte fluid, archegonial chamber fluid had elevated pH as well as higher osmolality.
Buying Time with Runnels: a Climate Adaptation Tool for Salt Marshes
Authors: Besterman Alice F. , Jakuba Rachel W. , Ferguson Wenley , Brennan Diana , Costa Joseph E. , Deegan Linda A. 
Published: January 2022

AbstractA prominent form of salt marsh loss is interior conversion to open water, driven by sea level rise in interaction with human activity and other stressors. Persistent inundation drowns vegetation and contributes to open water conversion in salt marsh interiors. Runnels are shallow channels originally developed in Australia to control mosquitoes by draining standing water, but recently used to restore marsh vegetation in the USA. Documentation on runnel efficacy is not widely available; yet over the past 10 years dozens of coastal adaptation projects in the northeastern USA have incorporated runnels. To better understand the efficacy of runnels used for restoration, we organized a workshop of 70 experts and stakeholders in coastal resource management. Through the workshop we developed a collective understanding of how runnels might be used to slow or reverse open water conversion, and identified unresolved questions. In this paper we present a synthesis of workshop discussions and results from a promising case study in which vegetation was restored at a degraded marsh within a few years of runnel construction. Despite case study outcomes, key questions remain on long-term runnel efficacy in marshes differing in elevation, tidal range, and management history. Runnel construction is unlikely to improve long-term marsh resilience alone, as it cannot address underlying causes of open water conversion. As a part of holistic climate planning that includes other management interventions, runnels may “buy time” for salt marshes to respond to management action, or adapt to sea level rise.
The Effects of ATIR Blocker on the Severity of COVID-19 in Hypertensive Inpatients and Virulence of SARS-CoV-2 in Hypertensive hACE2 Transgenic Mice
Authors: Jiang Xiaoliang , Li Huadong , Liu Yong , Bao Linlin , Zhan Lingjun , Gao Hong , Deng Wei , Xue Jing , Liu Jiangning , Liu Xing , Li Junli , Wang Jie , Wu Shuang , Yan Mingzhe , Luo Wei , Jose Pedro A. , Qin Chuan , Yang Xiuhong , Zhang Dingyu , Yang Zhiwei 
Published: January 2022

AbstractAngiotensin-converting enzyme 2 (ACE2) is required for the cellular entry of the severe acute respiratory syndrome coronavirus 2. ACE2, via the Ang-(1-7)-Mas-R axis, is part of the antihypertensive and cardioprotective effects of the renin-angiotensin system. We studied hospitalized COVID-19 patients with hypertension and hypertensive human(h) ACE2 transgenic mice to determine the outcome of COVID-19 with or without AT1 receptor (AT1R) blocker treatment. The severity of the illness and the levels of serum cardiac biomarkers (CK, CK-BM, cTnI), as well as the inflammation markers (IL-1, IL-6, CRP), were lesser in hypertensive COVID-19 patients treated with AT1R blockers than those treated with other antihypertensive drugs. Hypertensive hACE2 transgenic mice, pretreated with AT1R blocker, had increased ACE2 expression and SARS-CoV-2 in the kidney and heart, 1 day post-infection. We conclude that those hypertensive patients treated with AT1R blocker may be at higher risk for SARS-CoV-2 infection. However, AT1R blockers had no effect on the severity of the illness but instead may have protected COVID-19 patients from heart injury, via the ACE2-angiotensin1-7-Mas receptor axis.
Critical stages for post-harvest losses and nutrition outcomes in the value chains of bush beans and nightshade in Uganda
Authors: Strecker Klara , Bitzer Verena , Kruijssen Froukje 
Published: January 2022

AbstractThe reduction of post-harvest losses (PHLs) has been identified as a key pathway to food and nutrition security in sub-Saharan Africa. However, despite policy prioritisation, knowledge about the severity of PHLs remains scant, especially when it comes to nutrient-dense crops such as African nightshade and bush beans. Therefore, this paper identifies loss hotspots, causes and effects throughout the value chains of nightshade and bush beans in eastern Uganda. Primary data collected following the Informal Food Loss Assessment Method, combined with small-scale load tracking and secondary data, allows for an analysis of physical, economic, quality, and nutritional losses throughout the value chains of both crops. Results show that in the bush bean value chain, severe physical and quality losses occur during post-harvest handling by farmers, leading to high economic losses at this stage of the chain. Nutritional losses are not expected to be significant in the bush bean value chain. By contrast, due to the shortness of the nightshade value chain, where produce is moved from harvest to consumption within one or two days, physical losses in most parts of the chain are relatively minor. Only at consumption stage, high physical losses occur. This is also the stage where economic losses and potential nutritional losses are most pronounced. The results of this study offer a deeper understanding of the value chain dynamics of bush beans and nightshade, including underlying gender relations, and identify concrete loss hotspots, upon which further research and practical interventions can build.
WaveRange: wavelet-based data compression for three-dimensional numerical simulations on regular grids
Authors: Kolomenskiy Dmitry , Onishi Ryo , Uehara Hitoshi 
Published: January 2022

Abstract
A wavelet-based method for compression of three-dimensional simulation data is presented and its software framework is described. It uses wavelet decomposition and subsequent range coding with quantization suitable for floating-point data. The effectiveness of this method is demonstrated by applying it to example numerical tests, ranging from idealized configurations to realistic global-scale simulations. The novelty of this study is in its focus on assessing the impact of compression on post-processing and restart of numerical simulations.

Graphical abstract
A moving liver phantom in an anthropomorphic thorax for SPECT MP imaging
Authors: Panagi S. , Hadjiconstanti Α. , Charitou G. , Kaolis D. , Petrou I. , Kyriacou C. , Parpottas Y. 
Published: January 2022

AbstractCranio-caudal respiratory motion and liver activity cause a variety of complex myocardial perfusion (MP) artifacts, especially in the inferior myocardial wall, that may also mask cardiac defects. To assess and characterise such artifacts, an anthropomorphic thorax with moving thoracic phantoms can be utilised in SPECT MP imaging. In this study, a liver phantom was developed and anatomically added into an anthropomorphic phantom that also encloses an ECG beating cardiac phantom and breathing lungs’ phantom. A cranio-caudal respiratory motion was also developed for the liver phantom and it was synchronised with the corresponding ones of the other thoracic phantoms. This continuous motion was further divided into isochronous dynamic respiratory phases, from end-exhalation to end-inspiration, to perform SPECT acquisitions in different respiratory phases. The new motions’ parameters and settings were measured by mechanical means and also validated in a clinical environment by acquiring CT images and by using two imaging software packages. To demonstrate the new imaging capabilities of the phantom assembly, SPECT/CT MP acquisitions were performed and compared to previous phantom and patients studies. All thoracic phantoms can precisely perform physiological motions within the anthropomorphic thorax. The new capabilities of the phantom assembly allow to perform SPECT/CT MP acquisitions for different cardiac-liver activity ratios and cardiac-liver proximities in supine and, for first time, in prone position. Thus, MP artifacts can be characterised and motion correction can be performed due to these new capabilities. The impact of artifacts and motion correction on defect detection can be also investigated.
Long-lasting rescue of schizophrenia-relevant cognitive impairments via risperidone-loaded microPlates
Authors: Bellotti Elena , Contarini Gabriella , Geraci Federica , Torrisi Sebastiano Alfio , Piazza Cateno , Drago Filippo , Leggio Gian Marco , Papaleo Francesco , Decuzzi Paolo 
Published: January 2022

AbstractSchizophrenia is a disorder characterized by cognitive impairment and psychotic symptoms that fluctuate over time and can only be mitigated with the chronic administration of antipsychotics. Here, we propose biodegradable microPlates made of PLGA for the sustained release of risperidone over several weeks. Two microPlate configurations – short: 20 × 20 × 10 μm; tall: 20 × 20 × 20 μm – are engineered and compared to conventional ~ 10 μm PLGA microspheres in terms of risperidone loading and release. Tall microPlates realize the slowest release documenting a 35% risperidone delivery at 100 days with a residual rate of 30 ng/ml. Short microPlates and microspheres present similar release profiles with over 50% of the loaded risperidone delivered within the first 40 days. Then, the therapeutic efficacy of one single intraperitoneal injection of risperidone microPlates is compared to the daily administration of free risperidone in heterozygous knockout mice for dysbindin-1, a clinically relevant mouse model of cognitive and psychiatric liability. In temporal order object recognition tasks, mice treated with risperidone microPlates outperform those receiving free risperidone up to 2, 4, 8, and 12 weeks of observation. This suggests that the sustained release of antipsychotics from one-time microPlate deposition can rescue cognitive impairment in dysbindin mice for up to several weeks. Overall, these results demonstrate that risperidone-loaded microPlates are a promising platform for improving cognitive symptoms associated to schizophrenia. Moreover, the long-term efficacy with one single administration could be of clinical relevance in terms of patient’s compliance and adherence to the treatment regimen.
Graphical abstract
Single injection of long-acting risperidone-loaded µPL ameliorates the dysbindin-induced deficit in a clinically relevant mouse model of cognitive and psychiatric liability for up to 12 weeks
Factors affecting clinical manifestation of chromosomal imbalance in carriers of segmental autosomal mosaicism: differential impact of gender
Authors: Kovaleva Natalia V. , Cotter Philip D. 
Published: January 2022

Abstract
Mosaicism for unbalanced chromosomal rearrangements segmental mosaicism (SM) is rare, both in patients referred for cytogenetic testing and in prenatal diagnoses. In contrast, in preimplantation embryos SM is a frequent finding and, therefore, is even more challenging. However, there is no consistency among results of published studies on the clinical outcomes of embryos with SM, primarily due to the small number of reported cases. Moreover, there is the problem of predicting the potential for the optimal development of a mosaic embryo to a healthy individual. Therefore, we suggested comparing factors predisposing to favorable and poor prognoses, identified in postnatal and prenatal cohorts of SM carriers, with those obtained from studies on preimplantation embryos. We analyzed 580 published cases of SM including (i) postnatally diagnosed affected carriers, (ii) clinically asymptomatic carriers, (iii) prenatally diagnosed carriers, and (iv) miscarriages. We observed a concordance with preimplantation diagnoses regarding the clinical significance of the extent of mosaicism as well as a predominance of deletions over other types of rearrangements. However, there is no concordance regarding excessive involvement of chromosomes 1, 5, and 9 in unbalanced rearrangements and a preferential involvement of larger chromosomes compared to short ones. Paternal age was not found to be associated with SM in postnatally disease-defined individuals. We have identified maternal age and preferential involvement of chromosome 18 in rearrangements associated with clinical manifestations. Male predominance was found among normal pregnancy outcomes and among disease-defined carriers of rearrangements resulting in a gain of genomic material. Female predominance was found among abnormal pregnancy outcomes, among disease-defined carriers of loss and gain/loss rearrangements, and among transmitting carriers of gonadal SM, both affected and asymptomatic. According to data obtained from “post-embryo” studies, clinical manifestations of chromosomal imbalance are associated with a high proportion of abnormal cells, female gender, the type of rearrangement and involved chromosome(s), and maternal age. We believe these data are instructive in the challenging medical genetic counseling of parents faced with no option other than transfer of an embryo with segmental mosaicism.
Respiratory and cardiovascular effects of ambient particulate matter from dust storm and non-dust storm periods in Kuwait
Authors: Walters D. M. , Al-Khulaifi N. M. , Rushing B. R. , Selim M. I. 
Published: January 2022

AbstractEpidemiological studies demonstrate a positive association between daily changes in concentrations of ambient airborne particulate matter (PM) and adverse respiratory and cardiovascular health effects. However, physicochemical properties of PM can vary greatly across geographical, atmospheric, and temporal conditions and influence the relative toxicity of airborne PM. The purpose of this study was to investigate the adverse pulmonary and cardiovascular health effects of ambient PM collected from discrete sampling sites in Kuwait during dust storm (DS) and non-dust storm (NDS) conditions. Collected dust samples were characterized for their chemical composition using atomic absorption, GC–MS, and HPLC–MS analyses. Male BALB/cJ mice were exposed to 100 µg of either NDS or dust storm (DS) PM in 50 µl of PBS by oropharyngeal aspiration. Lung function was measured and bronchoalveolar lavage was conducted at 1, 7, and 14 days post-exposure. Ischemia–reperfusion injury was performed 24 h after exposures by obstructing the left main coronary artery approximately 4 mm distal to its origin for 20 min, followed by 2 h. of reperfusion. Exposure to either NDS or DS PM resulted in airway hyperresponsiveness to acetylcholine compared to PBS controls. Total protein and cells in BAL fluid were elevated in both dust groups one day after exposure; however, DS PM induced a greater increase in cell numbers than did NDS PM, particularly in neutrophils, eosinophils, and lymphocytes. Representative lung sections exhibited positive staining for mucus in large airways at 7 days which resolved by 14 days in dust storm-exposed mice but persisted in NDS-exposed animals. Our findings suggest that NDS PM may be more effective in producing an adaptive immune response, while the early inflammation induced by DS PM may better resolve. We also observed a prolonged airway mucus response after exposure to NDS PM, suggesting it may produce more asthma-like features than dust storm PM. PM-induced changes to cardiac ischemia–reperfusion injury were not observed in this study. The lack of cardiovascular response may have been due to the limited exposure and single time point used in this study.
A comparative study of small-scale fishery supply chains’ vulnerability and resilience to COVID-19
Authors: Bassett Hannah R. , Sharan Sonia , Suri Sharon K. , Advani Sahir , Giordano Christopher 
Published: January 2022

AbstractThe COVID-19 pandemic and response has significantly disrupted fishery supply chains, creating shortages of essential foods and constraining livelihoods globally. Small-scale fisheries (SSFs) are responding to the pandemic in a variety of ways. Together, disruptions from and responses to COVID-19 illuminate existing vulnerabilities in the fish distribution paradigm and possible means of reducing system and actor sensitivity and exposure and increasing adaptive capacity. Integrating concepts from literature on supply chain disruptions, social-ecological systems, human wellbeing, vulnerability, and SSFs, we synthesize preliminary lessons from six case studies from Indonesia, the Philippines, Peru, Canada, and the United States. The SSF supply chains examined employ different distribution strategies and operate in different geographic, political, social, economic, and cultural contexts. Specifically, we ask (a) how resilient have different SSF supply chains been to COVID-19 impacts; (b) what do these initial outcomes indicate about the role of distribution strategies in determining the vulnerability of SSF supply chains to macroeconomic shocks; and (c) what key factors have shaped this vulnerability? Based on our findings, systemic changes that may reduce SSF vulnerability to future macroeconomic shocks include: diversification of distribution strategies, livelihoods, and products; development of local and domestic markets and distribution channels; reduced reliance on international markets; establishment of effective communication channels; and preparation for providing aid to directly assist supply chains and support consumer purchasing power.