bluuebunny/crossref_metadata_2025_split huggingface dataset:
A three-year Herpetofauna survey from one of the largest remnants of the Atlantic Rainforest Biome (Reserva Natural Vale)
Authors: Pereira-Ribeiro Juliane , Ferreguetti Átilla Colombo , Bergallo Helena Godoy , Rocha Carlos Frederico Duarte 
Published: February 2022

Here we present a three-year survey of amphibian and reptile species registered in the Vale Natural Reserve (VNR), located in the north of Espírito Santo, southeastern Brazil. The VNR, along with the Sooretama Biological Reserve (SBR) and other surrounding areas, form a forest block that constitutes one of the largest remnants of continuous forest in the Atlantic Forest biome. We systematically sampled the herpetofauna community from 2015 to 2018, using the method of active search method in 27 plots of RAPELD distributed in the different types of vegetation present in the area. We recorded 39 species of amphibians belonging to the order Anura, distributed in seven families and 40 species of reptiles belonging to 20 families distributed in three orders: Crocodylia, Testudines and Squamata. The supplementation the samplings with those records from occasional encounters was important for the survey of the region's herpetofauna, mainly for reptiles. We added four species (two lizards, one amphisbaenid and one snake) to the known list for the VNR-SBR forest block: Micrablepharus maximiliani, Strobilurus torquatus, Amphisbaena alba and Tantilla sp. We demonstrated that VNR contains a high species richness of amphibians and reptiles, with a high number of anurans being endemic to the Atlantic Forest biome. The present species inventory provided the most current approximation of known the richness and composition of species of the existing herpetofauna at Vale Natural Reserve, since the period of the gathering of records is quite recent (2015-2018). Also, it brings the knowledge of some community parameters as species richness and composition not only for the whole area of the Vale Natural Reserve, but also for its different vegetation types, with no inclusion of environments outside this reserve. Furthermore, the presence of threatened species, the addition of four species to the current list, and the potential for new species to occur, reinforce the role of VNR as an important area for the protection of amphibian and reptile biodiversity in the Atlantic Forest.
El pueblo de la revolución. Las organizaciones armadas Argentinas en los años setenta
Authors: Slipak Daniela 
Published: February 2022

Taking Claude Lefort´s arguments as a base, this article analyzes practices and representations of the two most important military organizations of Argentina, Montoneros and the Partido Revolucionario de los Trabajadores-Ejército Revolucionario del Pueblo (PRT-ERP). Those non state organizations arose during the dictatorship governments of the Revolución Argentina (1966-1973) and incorporated militants to become the two insurgent groups with greater impact and political visibility of the time. A few years later, after the rule of another dictatorship government, the so called Proceso de Reorganización Nacional (1976-1983), Montoneros and the PRT-ERP felt in a breakdown and desestructration process. Studying documents, publications, statements and practices of Montoneros and the PRT-ERP, on one hand, this article questions about how this groups represented their political internal space and how they faced diversity and division of social relations. On the other hand, we tend to show the potential of Lefort’s reflections to understand the experience of armed groups in Latin America during the second half of twentieth century.
Candido: na trama das lembranças de Drummond e Murilo
Authors: Passos Cleusa Rios P. 
Published: February 2022

Em “Poesia e ficção autobiográfica”, Antonio Candido aborda a questão da memória em autores mineiros ao longo da história, detendo-se em Carlos Drummond de Andrade, Murilo Mendes e Pedro Nava. A leitura aqui proposta retoma algumas de suas ideias no primeiro e no segundo, elegendo, especificamente, os livros Boitempo I e A idade do serrote, para revelar os fragmentos do vivido, apreendidos a partir de “cenas, casos e emoções”, traços específicos do passado de ambos, transfigurados e ressignificados literariamente nas duas obras. E aí entra Candido que, ao sublinhar com agudez pontos fulcrais dos textos de cada um, oferece aos leitores várias direções interpretativas, inclusive a que estabelece relações sutis com a psicanálise. É esse viés que se busca perseguir aqui.
Osteomodulin Gene Expression Is Associated With Plaque Calcification, Stability, and Fewer Cardiovascular Events in the CPIP Cohort
Authors: Gonçalves Isabel , Oduor Loureen , Matthes Frank , Rakem Narjess , Meryn Jakob , Skenteris Nikolaos-Taxiarchis , Aspberg Anders , Orho-Melander Marju , Nilsson Jan , Matic Ljubica , Edsfeldt Andreas , Sun Jiangming , Bengtsson Eva 
Published: February 2022

Background:
Stable atherosclerotic plaques are characterized by thick fibrous caps of smooth muscle cells, collagen, and macrocalcifications. Identifying factors of plaque stability is necessary to design drugs to prevent plaque rupture and symptoms. Osteomodulin, originally identified in bones, is expressed by bone synthesizing osteoblasts and involved in mineralization. In the present study, we analyzed osteomodulin expression in human carotid plaques, its link with plaque phenotype, calcification, and future cardiovascular events.

Methods:

Osteomodulin gene expression
(OMD
; n=82) was determined by RNA sequencing and osteomodulin protein levels by immunohistochemistry (n=45) in carotid plaques obtained by endarterectomy from patients with or without cerebrovascular symptoms from the CPIP (Carotid Plaque Imaging Project) cohort, Skåne University Hospital, Sweden. Plaque components were assessed by immunohistochemistry, RNA sequencing, and multiplex analysis. Patients were followed for cardiovascular events or cardiovascular death during a median of 57 or 70 months, respectively, using national registers.

Results:

OMD
levels were increased in plaques from asymptomatic patients compared to symptomatics. High
OMD
levels were associated with fewer cardiovascular events during follow-up.
OMD
correlated positively with smooth muscle α-actin (
ACTA2
;
r
=0.73,
P
=10
-13
) and collagen (
COL1A2
;
r
=0.4,
P
=0.0002), but inversely with
CD68
gene expression (
r
=−0.67,
P
=10
-11
), lipids (
r
=−0.37,
P
=0.001), intraplaque hemorrhage (
r
=−0.32,
P
=0.010), inflammatory cytokine, and matrix metalloproteinase plaque contents.
OMD
was positively associated with
MSX2
(Msh Homeobox 2) (
r
=0.32,
P
=0.003), a marker of preosteoblast differentiation,
BMP4
(bone morphogenetic protein) (
r
=0.50,
P
=0.000002) and
BMP6
(
r
=0.47,
P
=0.000007), plaque calcification (
r
=0.35,
P
=0.016), and was strongly upregulated in osteogenically stimulated smooth muscle cells, which was further increased upon BMP stimulation. Osteomodulin protein was present in calcified regions. Osteomodulin protein levels were associated with plaque calcification (
r
=0.41,
P
=0.006) and increased in macrocalcified plaques.

Conclusions:
These data show that osteomodulin mRNA and protein levels are associated with plaque calcification in human atherosclerosis. Furthermore, osteomodulin mRNA, but not protein levels, is associated with plaque stability.
The World Soul and the Emergence of Human Life
Authors: Corrias Anna 
Published: February 2022

Abstract
Marsilio Ficino’s view on ensoulment, which can be extrapolated from his critique of natal astrology, relies on the relations of metaphysical proportion between the different levels of life and being which are central to Platonic philosophy. Drawing primarily on Plotinus, Ficino describes the emergence of life in the embryo as a process in which the World Soul is the true agent. For him, the ‘human nature’ that is present in the developing embryo attracts into the mother’s womb the seed of ‘human nature’ which is present in the World Soul’s vegetative power. Once channelled into the womb, the World Soul’s vegetative power animates the embryo’s body. Now endowed with organic life, the embryo becomes irresistibly attractive to the rational soul, which in this way is drawn down to it and becomes embodied. For Ficino, the moment when ‘rational’ ensoulment happens is completely irrelevant to prefiguring one’s life path, contrary to what was claimed by natal astrology. Nevertheless, it leaves an important mark on one’s physio-psychological makeup.
Growing Drama: A New Integral Paradigm through Children’s Drama Experience Construction in Early Childhood
Authors: Zhang Jinmei 
Published: February 2022

Abstract
Traditional drama education in early childhood has been concentrated on the interdisciplinary level, barely integrating children’s mind, body, and spirit. Language, science, society, health and art are integrated through drama to establish connections among the various disciplines. However, it is very important for children to walk between the worlds of reality and fiction through drama, interact with body and mind, and construct a complete experience. To achieve maximum integration of body and mind that is based on “education as growth” as in Dewey’s Experience and Education (1938), Growing Drama, a new integrated paradigm, maintains that the drama education of young children drama follow their drama experience construction.
Breaking the Ice: Applying Feminist Methodologies to Parliamentary Research in Uganda
Authors: Muzee Hannah 
Published: February 2022

Abstract
Even though feminism and gender studies have become the mainstream in academics, carrying out multi-disciplinary research in the social sciences and humanities through a feminist lens remains a challenge. Moreover, with the increased popularity of mixed research methods, the feminist epistemological standpoint tends to seem one-sided. The question then is whether feminist standpoint theorists take precedence over feminist empiricists. This paper sets out not only to explore the challenges of carrying out multi-disciplinary research through a feminist lens but also to explore the benefits of applying a feminist methodology to multi-disciplinary studies. Drawing from a study that sought to assess the factors that influenced the voices of women legislators during deliberations in the Ugandan Parliament, this paper reveals how the use of feminist methodologies exposed patterns that suggest why social change and the feminization of political spaces is important.
A biomarker panel for risk of early respiratory failure following hematopoietic cell transplantation
Authors: Rowan Courtney M. , Smith Lincoln , Sharron Matthew P. , Loftis Laura , Kudchadkar Sapna , Duncan Christine N. , Pike Francis , Carpenter Paul A. , Jacobsohn David , Bollard Catherine M. , Cruz Conrad Russell Y. , Malatpure Abhijeet , Farag Sherif , Renbarger Jamie , Little Morgan R. , Gafken Phillip R. , Krance Robert A. , Cooke Kenneth R. , Paczesny Sophie 
Published: February 2022

Abstract
Plasma biomarkers associated with respiratory failure (RF) following hematopoietic cell transplantation (HCT) have not been identified. Therefore, we aimed to validate early (7 and 14 days post-HCT) risk biomarkers for RF. Using tandem mass spectrometry, we compared plasma obtained at day 14 post-HCT from 15 patients with RF and 15 patients without RF. Six candidate proteins, from this discovery cohort or identified in the literature, were measured by enzyme-linked immunosorbent assay in day-7 and day-14 post-HCT samples from the training (n = 213) and validation (n = 119) cohorts. Cox proportional-hazard analyses with biomarkers dichotomized by Youden’s index, as well as landmark analyses to determine the association between biomarkers and RF, were performed. Of the 6 markers, Stimulation-2 (ST2), WAP 4-disulfide core domain protein 2 (WFDC2), interleukin-6 (IL-6), and tumor necrosis factor receptor 1 (TNFR1), measured at day 14 post-HCT, had the most significant association with an increased risk for RF in the training cohort (ST2: hazard ratio [HR], 4.5, P = .004; WFDC2: HR, 4.2, P = .010; IL-6: HR, 6.9, P &lt; .001; and TFNR1: HR, 6.1, P &lt; .001) and in the validation cohort (ST2: HR, 23.2, P = .013; WFDC2: HR, 18.2, P = .019; IL-6: HR, 12.2, P = .014; and TFNR1: HR, 16.1, P = .001) after adjusting for the conditioning regimen. Using cause-specific landmark analyses, including days 7 and 14, high plasma levels of ST2, WFDC2, IL-6, and TNFR1 were associated with an increased HR for RF in the training and validation cohorts. These biomarkers were also predictive of mortality from RF. ST2, WFDC2, IL-6 and TNFR1 levels measured early posttransplantation improve risk stratification for RF and its related mortality.
Association of peripheral immunity with cognition, neuroimaging, and Alzheimer’s pathology
Authors: Hou Jia-Hui , Ou Ya-Nan , Xu Wei , Zhang Peng-Fei , Tan Lan , Yu Jin-Tai ,   on behalf of Alzheimer’s Disease Neuroimaging Initiative
Published: February 2022

Abstract
Background
Neuroinflammation has been considered to be a driving force of Alzheimer’s disease. However, the association between peripheral immunity and AD has been rarely investigated.

Methods
Separate regression analyses were conducted to explore the associations among peripheral immune markers and cognition, neuroimaging, and AD pathology. Causal mediation analyses were used to investigate whether the associations with cognition were mediated by AD pathology.

Results
A total of 1107 participants (43.9% female, mean age of 73.2 years) from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) were included. Regression analyses indicated that elevated neutrophils (NEU) count and neutrophil-lymphocyte ratio (NLR) were associated with lower levels of global cognition, memory function (MEM), and executive function (EF), and reduced brain metabolism by 18F-fluorodeoxyglucose-positron emission tomography (FDG-PET) as well as greater ventricular volume. An elevated NLR was associated with a lower level of β-amyloid (Aβ) and a higher level of total tau (T-tau) in cerebrospinal fluid (CSF), smaller hippocampal volume (HV), and lesser entorhinal cortex (EC) thickness. On the contrary, an elevated level of lymphocytes (LYM) was associated with a higher level of Aβ and a lower level of T-tau in CSF, better cognition, and less atrophy of brain regions (ventricular volume, HV, and EC thickness). The associations of LYM and NLR with cognition were mediated by Aβ and T-tau pathology (proportion: 18%~64%; p < 0.05).

Conclusions
We revealed that two types of peripheral immune cells (NEU and LYM) and the ratio of these two cell types (NLR) had associations with cognition, neuroimaging, and AD pathology. The associations might be mediated by Aβ and tau pathology.
Blood and brain transcriptome analysis reveals APOE genotype-mediated and immune-related pathways involved in Alzheimer disease
Authors: Panitch Rebecca , Hu Junming , Xia Weiming , Bennett David A. , Stein Thor D. , Farrer Lindsay A. , Jun Gyungah R. 
Published: February 2022

AbstractBackgroundWhile Alzheimer disease (AD) is generally considered as a brain disorder, blood biomarkers may be useful for the diagnosis and prediction of AD brain pathology. TheAPOEε4 allele has shown cerebrovascular effects including acceleration of blood-brain barrier (BBB) breakdown.MethodsWe evaluated the differential expression of previously established AD genes in brains from 344 pathologically confirmed AD cases and 232 controls and in blood from 112 pathologically confirmed AD cases and 67 controls from the Religious Orders Study and Memory and Aging Project. Differential gene expression between AD cases and controls was analyzed in the blood and brain jointly using a multivariate approach in the total sample and withinAPOEgenotype groups. Gene set enrichment analysis was performed withinAPOEgenotype groups using the results from the combined blood and brain analyses to identify biologically important pathways. Gene co-expression networks in brain and blood samples were investigated using weighted correlation network analysis. Top-ranked genes from networks and pathways were further evaluated with vascular injury traits.ResultsWe observed differentially expressed genes withP< 0.05 in both brain and blood for established AD genesINPP5D(upregulated) andHLA-DQA1(downregulated).PIGHP1andFRAS1were differentially expressed at the transcriptome-wide level (P< 3.3 × 10−6) within ε2/ε3 and ε3/ε4 groups, respectively. Gene set enrichment analysis revealed 21 significant pathways (false discovery rateP< 0.05) in at least oneAPOEgenotype group. Ten pathways were significantly enriched in the ε3/ε4 group, and six of these were unique to these subjects. Four pathways (allograft rejection, interferon gamma response, peroxisome, and TNFA signaling via NFKB) were enriched for AD upregulated genes in the ε3/ε4 group and AD downregulated genes in subjects lacking ε4. We identified a co-expressed gene network in the brain that reproduced in blood and showed higher average expression in ε4 carriers. Twenty-three genes from pathway and network analyses were significantly associated with at least one vascular injury trait.ConclusionThese results suggest that theAPOEgenotype contributes to unique expression network profiles in both blood and brain. Several genes in these networks are associated with measures of vascular injury and potentially contribute to ε4’s effect on the BBB.
Long persistence of severe acute respiratory syndrome coronavirus 2 swab positivity in a drowned corpse: a case report
Authors: Bonelli Martina , Rosato Enrica , Locatelli Marcello , Tartaglia Angela , Falco Pietro , Petrarca Claudia , Potenza Francesca , Damiani Verena , Mandatori Domitilla , De Laurenzi Vincenzo , Stuppia Liborio , D’Ovidio Cristian 
Published: February 2022

Abstract
Background
Since the beginning of the worldwide spread of severe acute respiratory syndrome coronavirus 2 to date, important knowledge has been obtained about the virus behavior in living subjects and on inanimate surfaces; however, there is still a lack of data on virus persistency on dead bodies and the risk of contagion from cadavers.

Case presentation
The present case shows the persistency of the severe acute respiratory syndrome coronavirus 2 viral genome in nasopharyngeal swabs performed on a drowned Caucasian man, aged 41 years old, who was completely asymptomatic when he was alive, up to 41 days after death. Specific real-time reverse transcriptase-polymerase chain reaction (TaqMan 2019-nCoV Assay Kit v2; Thermo Fisher Scientific, Italy and Realquality RQ-SARS-CoV-2, AB Analytical) was used to evaluate the swabs.

Conclusions
This data reflect the importance of postmortem swabs in all autopsy cases, and not only in potential severe acute respiratory syndrome coronavirus 2-related death, and also highlight the necessity to evaluate virus positivity a long time after the moment of death, even if a low initial viral load was assessed.
Intratumoural spatial distribution of S100B + folliculostellate cells is associated with proliferation and expression of FSH and ERα in gonadotroph tumours
Authors: Ilie Mirela Diana , Vasiljevic Alexandre , Chanal Marie , Gadot Nicolas , Chinezu Laura , Jouanneau Emmanuel , Hennino Ana , Raverot Gérald , Bertolino Philippe 
Published: February 2022

AbstractFolliculostellate cells are S100B-expressing cells with numerous functions in the normal anterior pituitary. These cells have also been identified in pituitary neuroendocrine tumours (PitNETs), where their precise role remains elusive. Here, we aimed to build a refined cartography of S100B-expressing cells to characterise their interpatient and intratumoural spatial distribution, and to start identifying their potential functions in PitNETs. High-throughput histological analysis of S100B-stained tumour sections of 54 PitNETs revealed a significant decrease in S100B + cells in PitNETs compared to the normal anterior pituitary. A Ki67 index ≥ 3, a mitosis count > 2/10 per high power fields, and a proliferative status, were all associated with fewer S100B + cells in gonadotroph tumours. Gonadotroph tumours also showed interpatient and intratumoural heterogeneity in the spatial distribution of S100B + cells. The existence of an intratumoural heterogeneity was further confirmed by the incorporation to our spatial analysis of additional markers: Ki67, FSH, LH, ERα and SSTR2. The tumour areas with fewer S100B + cells displayed a higher percentage of Ki67 + cells, whereas strong positive correlations were observed between S100B + , FSH + , and ERα + cells. Such spatial associations suggest that S100B + folliculostellate cells could play a role in gonadotroph tumorigenesis, and may contribute to the maintenance of tumour cells in a low proliferating, FSH + /ERα + differentiated state. Albeit, further in-depth functional studies are required to decipher the mechanisms underlying these spatial associations and to potentially identify a therapeutic use.
On the performance of position-domain sidereal filter for 30-s kinematic GPS to mitigate multipath errors
Authors: Itoh Yuji , Aoki Yosuke 
Published: February 2022

AbstractThe noise level of kinematic Global Positioning System (GPS) coordinates is much higher than static daily coordinates. Therefore, it needs to be improved to capture details of small sub-daily tectonic deformation. Multipath is one of the dominant error sources of kinematic GPS, which the sidereal filter can mitigate. With increasing interest in applying kinematic GPS to early postseismic deformation studies, we investigate the characteristics of multipath errors and the performance of the position-domain sidereal filter using 30-s kinematic coordinates with a length of nearly 5 days. Experiments using three very short baselines mostly free from atmospheric disturbances show that multipath signature in position-domain has better repeatability at longer periods, and sidereal filtering without low-pass filtering yields a lift of power spectral density (PSD) at periods shorter than 200 s. These results recommend an empirical practice of low-pass filtering to a sidereal filter. However, a moderate cut-off period maximizes the performance of the sidereal filter because of the smaller multipath signature at longer periods. The amplitude of post-sidereal-filtered fluctuation is less than 6 mm in standard deviation, which demonstrates the nearly lowest noise level of kinematic GPS used for postseismic and other tectonic deformation studies. Our sidereal filter is proven to mitigate several peaks of power spectral density at periods up to 100,000 s, but the period dependency of PSD is not fully alleviated by sidereal filtering, which needs future investigation.
Graphical Abstract