bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optogenetic inhibition of the dorsal hippocampus CA3 region during early-stage cocaine-memory reconsolidation disrupts subsequent context-induced cocaine seeking in rats
Authors: Qi Shuyi , Tan Shi Min , Wang Rong , Higginbotham Jessica A. , Ritchie Jobe L. , Ibarra Christopher K. , Arguello Amy A. , Christian Robert J. , Fuchs Rita A. 
Published: December 2021

ABSTRACTThe dorsal hippocampus (DH) is key to the long-term maintenance of cocaine memories following retrieval-induced memory destabilization; even though, it is not the site of protein synthesis-dependent memory reconsolidation. Here, we took advantage of the temporal and spatial specificity of an optogenetic manipulation to examine the role of the cornu ammonis 3 subregion of the DH (dCA3) in early-stage cocaine-memory reconsolidation. Male Sprague-Dawley rats expressing eNpHR3.0 in the DH were trained to self-administer cocaine in a distinct context and underwent extinction training in a different context. Rats then received a 15-min memory-reactivation session, to destabilize cocaine memories and trigger reconsolidation, or remained in their home cages (no-reactivation controls). Optogenetic inhibition of the dCA3 for 1 h immediately, but not 1 h, after memory reactivation resulted in cocaine-memory impairment as indicated by reduction in drug-seeking behavior selectively in the cocaine-paired context 3 d later, at test, relative to responding in no-inhibition, no-reactivation, and no-eNpHR3.0 controls. Cocaine-memory impairment was associated with reduced c-Fos expression, an index of neuronal activation, in the dCA3 stratum lucidum (SL) and stratum pyramidale (SP) at test. Based on these observations and extant literature, we postulate that recurrent circuits in the SP are activated during early-stage memory reconsolidation to maintain labile cocaine memories prior to protein synthesis-dependent restabilization in another brain region, such as the basolateral amygdala. Furthermore, SL and SP interneurons may enhance memory reconsolidation by limiting synaptic noise in the SP and also contribute to recall as elements of the updated cocaine engram or retrieval links.
Tissue Registration and Exploration User Interfaces in support of a Human Reference Atlas
Authors: Börner Katy , Bueckle Andreas , Herr Bruce W. , Cross Leonard E. , Quardokus Ellen M. , Record Elizabeth G. , Ju Yingnan , Silverstein Jonathan C. , Browne Kristen M. , Jain Sanjay , Wasserfall Clive H. , Jorgensen Marda L. , Spraggins Jeffrey M. , Patterson Nathan H. , Weber Griffin M. 
Published: December 2021

AbstractSeveral international consortia are collaborating to construct a human reference atlas, which is a comprehensive, high-resolution, three-dimensional atlas of all the cells in the healthy human body. Laboratories around the world are collecting tissue specimens from donors varying in sex, age, ethnicity, and body mass index. However, integrating and harmonizing tissue data across 20+ organs and more than 15 bulk and spatial single-cell assay types poses diverse challenges. Here we present the software tools and user interfaces developed to annotate (“register”) and explore the collected tissue data. A key part of these tools is a common coordinate framework, which provides standard terminologies and data structures for describing specimens, biological structures, and spatial positions linked to existing ontologies. As of December 2021, the “registration” user interface has been used to harmonize and make publicly available data on 6,178 tissue sections from 2,698 tissue blocks collected by the Human Biomolecular Atlas Program, the Stimulating Peripheral Activity to Relieve Conditions program, the Human Cell Atlas, the Kidney Precision Medicine Project, and the Genotype Tissue Expression project. The second “exploration” user interface enables consortia to evaluate data quality and coverage, explore tissue data in the context of the human body, and guide data acquisition.
Information encoded in volumes and areas of dendritic spines is nearly maximal across mammalian brains
Authors: Karbowski Jan , Urban Paulina 
Published: December 2021

AbstractMany experiments suggest that long-term information associated with neuronal memory resides collectively in dendritic spines. However, spines can have a limited size due to metabolic and neuroanatomical constraints, which should effectively limit the amount of encoded information in excitatory synapses. This study investigates how much information can be stored in the population of sizes of dendritic spines, and whether it is optimal in any sense. It is shown here, using empirical data for several mammalian brains across different regions and physiological conditions, that dendritic spines nearly maximize entropy contained in their volumes and surface areas for a given mean size in cortical and hippocampal regions. Although both short- and heavy-tailed fitting distributions approach 90 − 100% of maximal entropy in the majority of cases, the best maximization is obtained primarily for short-tailed gamma distribution. We find that most empirical ratios of standard deviation to mean for spine volumes and areas are in the range 1.0 ± 0.3, which is close to the theoretical optimal ratios coming from entropy maximization for gamma and lognormal distributions. On average, the highest entropy is contained in spine length (4−5 bits per spine), and the lowest in spine volume and area (2 − 3 bits), although the latter two are closer to optimality. In contrast, we find that entropy density (entropy per spine size) is always suboptimal. Our results suggest that spine sizes are almost as random as possible given the constraint on their size, and moreover the general principle of entropy maximization is applicable and potentially useful to information and memory storing in the population of cortical and hippocampal excitatory synapses, and to predicting their morphological properties.Significance statementIt is believed that information related to long-term memory is stored collectively in the parts of excitatory synapses called dendritic spines. But how efficient is the information capacity given synaptic size variability? Generally, the larger this variability the higher entropy (information capacity) of spine population. However, this process comes at some cost, as larger synapses use more metabolic energy and brain tissue, suggesting a benefit-cost trade-off for storing long-term information. We show that volumes and areas of actual spines in many different parts of the brain across different mammals and conditions adjust simultaneously their variable mean and standard deviation values to nearly maximize entropy of their distributions. This suggests that storing capacity of dendritic spines is nearly maximal, despite large variability in their sizes.
iTRVZ: liquid nano-platform for signal integration on the plasma membrane
Authors: Tsunoyama Taka A. , Hoffmann Christian , Tang Bo , Hirosawa Koichiro M. , Nemoto Yuri L. , Kasai Rinshi S. , Fujiwara Takahiro K. , Suzuki Kenichi G.N. , Milovanovic Dragomir , Kusumi Akihiro 
Published: December 2021

SUMMARYCrosstalk of cellular signaling pathways is essential for coordinating cell responses. Therefore, we suspect the existence of special scaffolding platforms that specifically undertake signal crosstalk and integration. Using advanced single-molecule imaging, we found a nanometer-scale liquid-like platform for integrating the signals downstream from GPI-anchored receptors and receptor-type tyrosine kinases. The platform employs some of the focal adhesion proteins, including integrin, talin, RIAM, VASP, and zyxin, but is distinct from focal adhesions, and is thus termed iTRVZ. The iTRVZ formation is driven by the protein liquid-liquid phase separation and the interactions with the raft domains in the plasma membrane and cortical actin. iTRVZ non-linearly integrates the two distinctly different receptor signals, and thus works as an AND gate and noise filter.HighlightsIntegrin, talin, RIAM, VASP, and zyxin are clustered which called iTRVZiTRVZ forms liquid-like nanoscale cluster throughout the plasma membraneDownstream molecules are recruited to iTRVZ upon different receptor stimulationsPhase separation is critical for iTRVZ formation and its signal transductionTwo weak inputs are integrated in iTRVZ and non-linearly amplified to strong output
Cell-free chromatin particles released from dying cells inflict mitochondrial damage and ROS production in living cells
Authors: Raghuram Gorantla V , Tripathy Bhabesh Kumar , Avadhani Kartikeya , Shabrish Snehal , Khare Naveen Kumar , Lopes Relestina , Pal Kavita , Mittra Indraneel 
Published: December 2021

AbstractmtDNA damage and the resultant oxidative stress are associated with neurodegenerative diseases, ageing and cancer. However, what triggers mtDNA damage remains unclear. We have reported that cell-free chromatin particles (cfChPs) that are released from the billions of cells that die in the body every day can readily enter into healthy cells and damage their DNA. We show here that cfChPs isolated from sera of healthy individuals, or those that are released from dying cells, inflict direct physical damage mtDNA leading to marked activation of ROS. The latter could be abrogated by concurrent treatment with three different cfChPs deactivating agents. Given that 1×109-1×1012cells die in the body every day, our findings suggest that cfChPs from dying cells are major physiological triggers for mtDNA damage and ROS production. Deactivation of cfChPs may provide a novel therapeutic approach to retard ageing and associated degenerative conditions that have been linked to oxidative stress.
Map-independent representation of an aggression-promoting social cue in the main olfactory pathway
Authors: Cichy Annika , Dewan Adam , Zhang Jingji , Kaye Sarah , Teng Tiffany , Blanchard Kassandra , Feinstein Paul , Bozza Thomas 
Published: December 2021

AbstractWhile the olfactory system is required for proper social behaviors, the molecular basis for how social cues are detected via the main olfactory pathway of mammals is not well-characterized. Trimethylamine is a volatile, sex-specific odor found in adult male mouse urine that selectively activates main olfactory sensory neurons that express trace amine-associated receptor 5 (TAAR5). Here we show that trimethylamine, acting via TAAR5, elicits state-dependent attraction or aversion in male mice and drives inter-male aggression. Genetic knockout of TAAR5 significantly reduces aggression-related behaviors, while adding trimethylamine augments aggressive behavior towards juvenile males. We further show that transgenic expression of TAAR5 specifically in olfactory sensory neurons rescues aggressive behaviors in knockout mice, despite extensive remapping of TAAR5 projections to the olfactory bulb. Our results identify a specific main olfactory input that detects a prominent male-specific odor to induce inter-male aggression in a mammalian species and reveal that apparently innate behavioral responses are independent of patterned glomerular input to the olfactory bulb.
Contextual and linguistic challenges for French business schools to achieve international accreditation: experts as boundary-spanners
Authors: Vigier Mary , Bryant Michael 
Published: December 2021

PurposeThe purpose of this paper is to explore the contextual and linguistic challenges that French business schools face when preparing for international accreditation and to shed light on the different ways in which experts facilitate these accreditation processes, particularly with respect to how they capitalize on their contextual and linguistic boundary-spanning competences.Design/methodology/approachThe authors interviewed 12 key players at four business schools in France engaged in international accreditations and in three specific categories: senior management, tenured faculty and administrative staff. The interview-based case study design used semi-structured questions and an insider researcher approach to study an underexplored sector of analysis.FindingsThe findings suggest that French business schools have been particularly impacted by the colonizing effects of English as the mandatory language of the international accreditation bodies espousing a basically Anglophone higher education philosophy. Consequently, schools engage external experts for their contextual and linguistic boundary-spanning expertise to facilitate accreditation processes.Originality/valueThe authors contribute to language-sensitive research through a critical perspective on marginalization within French business schools due to the use of English as the mandatory lingua franca of international accreditation processes and due to the underlying higher-education philosophy from the Anglophone academic sphere within these processes. As a result, French business schools resort to external experts to mediate their knowledge and competency gaps.
The moderating effect of coworkers’ training participation on the influence of peer support in the transfer process
Authors: Salamon Janos , Blume Brian D. , Orosz Gábor , Nagy Tamás 
Published: December 2021

Purpose
The impact of the number of coworkers participating in training on transfer outcomes has largely been overlooked. This paper aims to examine whether the number of coworkers participating in training interacts with peer support (PS) to influence training motivation and transfer.

Design/methodology/approach
Data were collected using a cross-sectional survey from a sample of 688 employees working in 14 midsize and large companies. All participants were recent trainees in various open skill (e.g. leadership) training programs. Moderated mediation was used to test the hypotheses.

Findings
Motivation to transfer (MTT) mediated the relationship between PS and perceived training transfer. When more coworkers participated in the training, PS had a stronger influence on trainee MTT.

Practical implications
Organizations should consider training coworker cohorts at the same time to influence MTT and training transfer. Generally, whole-team training programs could be used to boost training transfer outcomes, although it could potentially have a negative impact on transfer if PS is low.

Originality/value
To the best of the authors’ knowledge, this was the first study to demonstrate that the number of coworkers participating in training can moderate the effect of PS on MTT and training transfer.
Suggestions for large-scale, postdisaster reconstruction involving indigenous populations: a participatory approach to recovery after Typhoon Morakot
Authors: Tsai Sung Lun , Ochiai Chiho , Tseng Min Hui , Deng Chuan Zhong 
Published: December 2021

Purpose
The participatory method, a major factor for a successful post-disaster reconstruction (PDR) project, is applied in various stages of the PDR. However, the application of this method for PDR involving indigenous populations is underexplored. Therefore, this paper aims to analyze the critical factors that can influence the participatory PDR in the indigenous context.

Design/methodology/approach
Two large-scale, indigenous, post-disaster relocation projects after the 2009 Typhoon Morakot were selected as case studies. The qualitative and quantitative methodology (semi-structured interview and questionnaire) were applied in the research.

Findings
A participation-friendly policy, community organization, the extent of damage, flexibility of nongovernmental organizations, understanding of the participatory concept and mutual trust were found to be essential factors that profoundly influence participation in PDR projects.

Originality/value
This study contributes by providing guidelines for future participatory PDR projects, especially in the indigenous context.
Cooperative education during Covid-19 pandemic: enhancing legal rights and professional development of interns in Thailand
Authors: Pathak Shubham , Laikram Siwarut 
Published: December 2021

Purpose
The study aims at enriching the existing cooperative education sector in Thailand. Adequate cooperative education has direct impacts upon graduates’ future professional development, employability and enhanced professional skills. The cooperative education framework in Thailand is relatively a recent concept and lacks detailed research.

Design/methodology/approach
The methodology adopted in this study is mixed-method, inclusive of qualitative methodology where data were collected through key informant interviews and; quantitative methodology involving survey questionnaires with a sample of 350 respondents. The data analysis included the quantitative analysis with Chi-square and excerpts from the key informant interview respondents. Additional strength, weakness, opportunity and threat analysis is performed to provide for gaps at various levels of cooperative education and the potential opportunities to the graduates in Thailand.

Findings
The findings depict a lack of legal framework for effective skill development, uncertain moral and physical security of the interns and absence of legal rights for interns, minimal support and assistance from the government sector, reduced future employability and insufficient financial resources among poorer students.

Research limitations/implications
Lack of student and cooperative database with the government departments.

Practical implications
The Thai Qualification Framework has been adopted in a majority of universities, however, the quality assurance does not cover the student’s perspectives, financial and social limitations towards attending the cooperative education.

Social implications
With enhanced vulnerabilities due to the Covid-19 pandemic, adverse impacts are analysed and recommendations are provided for enhancing cooperative education opportunities towards students.

Originality/value
This research aims to understand the perspectives of the students who graduated with cooperative education and are currently working professionals.
Exploring differences in the antisocial behaviors of adolescent rule-breaking that affect entrepreneurial persistence
Authors: Randolph Angela F. , Greenberg Danna , Simon Jessica K. , Gartner William B. 
Published: December 2021

PurposeThe authors explore the relationship between adolescent behavior and subsequent entrepreneurial persistence by drawing on scholarship from clinical psychology and criminology to examine different subtypes of antisocial behavior (nonaggressive antisocial behavior and aggressive antisocial behavior) that underlie adolescent rule breaking. The intersection of gender and socioeconomic status on these types of antisocial behavior and entrepreneurial persistence is also studied.Design/methodology/approachUsing a longitudinal research design, this study draws from a national representative survey of USA adolescents, the National Longitudinal Survey of Youth (1997) (NLSY97). Nonaggressive antisocial behavior was assessed with a composite scale that measured economic self-interest and with a second measure that focused on substance abuse. Aggressive antisocial behavior was assessed as a measure of aggressive, destructive behaviors, such as fighting and property destruction. Entrepreneurial persistence was operationalized as years of self-employment experience, which is based on the number of years a respondent reported any self-employment.FindingsAggressive antisocial behavior is positively related to entrepreneurial persistence but nonaggressive antisocial behavior is not. This relationship is moderated by gender and socioeconomic status.Originality/valueThese findings contribute to research on the relationship between adolescent behavior and entrepreneurship in adulthood, the effect of antisocial behavior, and demographic intersectionality (by gender and socioeconomic status) in entrepreneurship. The authors surmise that the finding that self-employment for men from lower socioeconomic backgrounds involved in aggressive antisocial behavior was significantly higher compared to others may indicate that necessity entrepreneurship may be the primary driver of entrepreneurial activity for these individuals.
Reflective backward analysis to assess the operational performance and eco-efficiency of two industrial districts
Authors: Appolloni Andrea , D'Adamo Idiano , Gastaldi Massimo , Yazdani Morteza , Settembre-Blundo Davide 
Published: December 2021

PurposeThe best strategy to apply for the future cannot disregard a careful analysis of the past and is the one capable of seizing opportunities from outside. Manufacturing sectors are characterized by sudden changes, and in this work, we analyze the ceramic tiles sector characterized by a mature technology in which innovation has played a key role.Design/methodology/approachThis study aims to provide a sectorial analysis based on a historical data set (2004–2019) to highlight how an industry is performing both operationally and in terms of eco-efficiency. For this purpose, from a methodological point of view, the data envelopment analysis (DEA) was used.FindingsThe results of the analysis show that the Spanish ceramics industry shows a growing economic trend by taking advantage of lower industrial costs, while the Italian industry is characterized by a modest decline partially mitigated by exports. The industrial districts are an aggregation of companies that in the ceramic sector has allowed to combine innovation, sustainability and digitalization and is a model toward the maximization of sustainable efficiency because it is a place of aggregation of resources and ideas.Originality/valueThis study experiments with an innovative way of addressing traditional industry analysis, namely, integrating the reflective management approach with DEA-based backward analysis. This provides decision makers with the basis for new interpretations of variable trends.
Exploring the life cycle of family-owned tourism businesses in maturity
Authors: López-Chávez Beatriz Adriana , Maldonado-Alcudia César 
Published: December 2021

PurposeThe aim of this paper is to analyze the life cycle of family-owned hotels in the maturity phase from the integration of theoretical models for family-owned tourism businesses.Design/methodology/approachA qualitative multiple case study was used to analyze four mature family-owned hotels through eight interviews and four observation guides with an abductive method. Three axes were analyzed; the ownership with the Gersick model, the family with the Tobak and Nábradí model and the business with the Butler tourist areas model to identify whether they are going through the consolidation stage, stagnation, rejuvenation or decline within its maturity.FindingsThe cases studied evolve in the three axes. In the business axes, two go through the stagnation stage, another in decline and the last in consolidation; all remain under controlling owners. In the family, there are different generations in charge. The boost to the destination plays a key role as a force for deterministic change in the internal transformation of these organizations, and to remain in consolidation, discontinuous changes and voluntaristic actions are necessary.Originality/valueFamily businesses seek longevity, although a low percentage reaches maturity. This research proposes the integration of life cycle models to understand its development in the axes of family, ownership and business, where aspects of the tourism industry are considered and allow the stage identification through which it passes in maturity, supporting internal decision making.