bluuebunny/crossref_metadata_2025_split huggingface dataset:
Significance of Tumor Deposits combined with Lymph Node Metastasis in Stage III Colorectal Cancer Patients: A retrospective multi-center cohort study from China
Authors: Pu Hongjiang , Pang Xiaolin , Fu Jiangping , Zheng Rui , Chen Yaxue , Zhang Dafu , Fang Xiangdong 
Published: March 2022

Abstract
Purpose
The study aimed to explore the value of tumor deposits in stage III colorectal cancer (CRC) and verify whether patients with more tumor deposit numbers have higher risk of recurrence.
Methods
The retrospective cohort analysis was performed at two cancer centers of China. Stage III CRC patients who underwent radical resection at the center between April 2008 and February 2019 were identified. The Univariate/Multivariate Cox regression, Kaplan-Meier analysis, and PSM were recurrence-free survival (RFS) used.
Results
Total 1080 stage III CRC patients (634 [58.7%] men; median [IQR] age, 60 [50–68] years) underwent radical surgical resection were identified for inclusion in this study. Patients with tumor deposits had a 12.8% lower 3-year RFS (n = 236 [69.9%]) than the patients without tumor deposits (n = 844 [82.7%]) (P ≤ 0.0001). The 3-year RFS of patients with stage N2 (n = 335 [61.2%]) was 18.6% lower (P ≤ 0.0001) than the original cohort of patients with stage N1 (n = 745 [79.8%]), but it was similar to the RFS of patients with 4 or more tumors deposits plus lymph node metastases (n = 58 [61.4%]) (P = 0.91). The RFS for patients with 4 or more tumor deposits plus number of lymph node metastases (n = 58 [61.4%]) was 15.8% lower than the cohort of patients with 1–3 tumor deposits + number of lymph node metastases (n = 687 [77.2%]) % (P = 0.001). Multivariate analysis confirmed that patients with 4 or more tumor deposits + the number of lymph node metastases (hazard ratio [HR], 1.88; 95% CI, 1.24–2.87) was independently associated with a shorter RFS.
Conclusion
The number of tumor deposits is an indicator of poor postoperative prognosis. It is necessary to incorporate the number of tumor deposits combined with the number of lymph node metastases to stratify postoperative stratification of stage III CRC, which may provide a new theoretical basis for adjuvant therapy for patients with N1 stage CRC after surgery.
Prediction of residual ischemic risk in ticagrelor-treated patients with acute coronary syndrome
Authors: Zou Yuting , Wang Yuyan , Wu Yangxun , Zhang Shizhao , Liu Haiping , Yin Tong 
Published: March 2022

Abstract
BackgroundDespite strong antiplatelet therapy with ticagrelor, serious ischemic events still occur in patients with acute coronary syndrome (ACS). The predictability of platelet reactivity to the residual risk of ischemic events during ticagrelor treatment remains uncertain.ObjectivesWe aimed to investigate the predictability of the thromboelastography (TEG)-measured adenosine disphosphate (ADP)-induced platelet inhibition rate (ADP%) to the ischemic events in ticagrelor-treated patients with ACS.MethodsA cohort of ticagrelor-treated patients with ACS were consecutively recruited. ADP% was measured by TEG after 3 days of ticagrelor maintenance treatment. The primary ischemic event was defined as rehospitalization for unstable angina (UA) within 1 year, and the secondary ischemic event was a composite of the primary ischemic event plus all-cause death, nonfatal myocardial infarction (MI), stent thrombosis, stroke, and unplanned revascularization within 1 year.ResultsA total of 751 eligible patients with ACS were included in the analysis, with 336 patients randomly assigned to the derivation group and 415 to the validation group. The overall rates of primary and secondary ischemic events were 14.51% (n=109) and 16.91% (n=127), respectively. Compared to the patients without ischemic events, those with ischemic events had a significantly lower ADP% both in the derivation group (for primary ischemic events: 66.05% vs. 92.80%, p<0.001; for secondary ischemic events: 66.05% vs. 93.20%, p<0.001) and in the validation group (for primary ischemic events: 66.40% vs. 89.20%, p<0.001; for secondary ischemic events: 66.90% vs. 89.20%, p<0.001). Receiver operating characteristic curve (ROC) analysis showed that an ADP% < 76% was the optimal cut-off value for predicting 1-year primary ischemic events, with an area under the curve (AUC) of 0.80 (95% CI: 0.72-0.86, p < 0.001) in the derivation group and 0.77 (95% CI: 0.69-0.85, p < 0.001) in the validation group. The multivariate Cox regression hazard analysis consistently identified an ADP% < 76% as an independent predictor of primary ischemic events in the derivation group (HR: 8.21, 95% CI: 4.82-13.99, p< 0.001) and in the validation group (HR: 6.34 95% CI: 3.32-12.11, p< 0.001). There was also a strong association between an ADP% <76% and the occurrence of secondary ischemic events in the derivation group (HR: 7.33, 95% CI: 4.47-12.00, p< 0.001) and in the validation group (HR: 4.76, 95% CI: 2.73-8.32, p< 0.001).ConclusionThe ADP-induced platelet inhibition rate measured by TEG could predict ischemic events in ticagrelor-treated patients with ACS.
Revenue loss due to whale entanglement mitigation and fishery closures
Authors: Seary Rachel , Santora Jarrod A , Tommasi Desiree , Thompson Andrew , Bograd Steven J , Richerson Kate , Brodie Stephanie , Holland Dan 
Published: March 2022

Abstract
Whale entanglements with fishing gear, exacerbated by changing environmental conditions, pose significant risk to whale populations. Management tools used to reduce entanglement risk, for example temporary area restrictions on fishing, can have negative economic consequences for fishing communities. Balancing whale protection with sustaining productive fisheries is therefore a challenge experienced worldwide. In the California Current Ecosystem, ecosystem indicators have been used to understand the environmental dynamics that lead to increased whale entanglement risk in a lucrative crab fishery. However, an assessment of socio-economic risk for this fishery, as in many other regions, is missing. We estimate retrospectively the losses from ex-vessel revenue experienced by commercial Dungeness crab fishers in California during two seasons subject to whale entanglement mitigation measures. In the 2020 fishing season, our results suggest total revenues would have been $14.4 million higher in the Central Management Area of California in the absence of closures and other disturbances. In the 2019 fishing season, our results suggest ex-vessel revenues would have been $9.4 million higher in the Central Management Area and $0.3 million higher in the Northern Management Area. Our evaluation should motivate the development of strategies which maximize whale protection whilst promoting productive, sustainable and economically-viable fisheries.
Sepsis-Induced Brain State Instability
Authors: Kala Annu , Leemburg Susan , Jezek Karel 
Published: March 2022

Abstract
Background
Sepsis-associated brain dysfunction (SABD) is a frequent severe complication of sepsis and the systemic inflammatory response syndrome. It is associated with high mortality and a majority of survivors suffer long-term neurological consequences. Sleep is commonly affected in sepsis and there is a strong correlation between its impairment and development of other complications or increased mortality in sepsis patients. Here, we investigate the effects of sepsis on brain activity patterns in order to better understand possible sources of sleep-wake disturbances associated with severe systemic inflammation.
Methods
We studied the effects of high LPS doses (10mg/kg) on oscillatory brain states in an acute rat model of sepsis under urethane anaesthesia, which maintains REM- and NREM sleep-like states. Twelve Long-Evans rats (6 LPS, 6 controls) were implanted with eight independently movable tetrodes in the dorsal hippocampus. Baseline LFP activity was recorded for 3 hours after saline injection, followed by another 3 hours after LPS or saline injection. REM and NREM were automatically classified based on LFP activity and quantified. Within- and between-state dynamics were analysed using a 2-D state space approach based on spectral power ratios. Aperiodic and periodic components of the power spectrum were quantified for each state. Blood serum samples and brains were collected for IL-1β quantification and histological verification of electrode placement.
Results
Soon after LPS injection we observed a robust fragmentation of both oscillatory states resulting in a three-fold increase in the number of state transitions that lasted for several hours, although the overall time spent in either state did not change. Analysis of power spectra showed opposing shifts in low frequency oscillations (1–9 Hz) in REM and NREM that resulted in increased similarity between both states in 2-D state space.
Conclusions
The observed increased spectral similarity between REM and NREM and increased instability within the states may point to a mechanism underlying the severe sleep fragmentation described both in sepsis patients and in SABD animal models.
Description of New Mite Pest (Acari: Eriophyidae) and Its Nature of Damage Found on Curry Leaf (Murraya Koengii Spr.) From West Bengal, India
Authors: Sarkar Sanjay 
Published: March 2022

Abstract
Among the Acari, Eriophyoid mites are second largest group with regard to their economic importance as phytophagous pest throughout the world [1] Lindquist et al., 1996). These mites are drawing the attention of biologist all over the world for their morphological and biological specializations. Due to their obligatory phytophagous mode of nutrition they exhibit marked host specificity. During the general surveys for eriophyoid mites from present study area a new species, Paratetra subhimalyana sp. nov. of the class Acari of the family Eriophyidae was found as vagrants on tender shoot of Murrya koengii Spr. This new phytophagous mite species was described and illustrated from the specimens collected at English bazar, Malda (25° 0' 39.0276'' N and 88° 8' 27.9528'' E.), West Bengal, India. Relationships of new species with other eriophyoid species are also provided. After proper diagnosis of the species it is found that this is the second eriohyoid mite species described from Murraya koengii Spr. in India and added new to science as a seventh species under the genus Paratetra. This mite causes visible damage symptoms to the host plant and appeared as a potential pest of Curry leaves in India. For proper control of the mite pest, the taxonomic study of this mite and their relation to the host plant is very much essential. Taxonomic descriptions of the new species and its nature of damage is discussed in this article.
Evaluation of the potential diagnostic role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA networks as non-invasive circulatory biomarkers in ductal carcinoma breast cancer
Authors: Razi Shokufeh , Mozdarani Hossein , Andouhjerdi Roudabeh Behzadi 
Published: March 2022

Abstract
Over the last few decades, tremendous progress has been achieved in the early detection and treatment of breast cancer. However, the prognosis remains unsatisfactory, and the underlying processes of carcinogenesis are still unclear. The purpose of this research was to find out the relationship between MIAT, FOXO3a, and miRNA29a-3p and evaluated the expression levels of them in breast cancer tissue compared to whole blood and their potential as a noninvasive biomarker in whole blood in breast cancer. Whole blood and breast cancer tissue are taken from patients before radiotherapy and chemotherapy. Total RNA was extracted from breast cancer tissue and whole blood to synthesize complementary DNA (cDNA). The expression of MIAT, FOXO3a and miRNA29a-3p was analyzed by the RT-qPCR method and the sensitivity and specificity of them were determined by the receiver operating characteristic (ROC) curve. Bioinformatics analysis was used to understand the connections between MIAT, FOXO3a, and miRNA29a-3p in human breast cancer in order to develop a ceRNA (competitive endogenous RNA) network. We identified that in ductal carcinoma breast cancer tissue and whole blood, MIAT and FOXO3a were more highly expressed, while miRNA29a-3p was lower compared with those in non-tumor samples. There was a positive correlation between the expression levels of MIAT, FOXO3a, and miRNA29a-3p in breast cancer tissues and whole blood. Our results also proposed miRNA29a-3p as a common target between MIAT and FOXO3a, and we showed them as a ceRNA network. This is the first study that indicates MIAT, FOXO3a, and miRNA29a-3p as a ceRNA network, and their expression was analyzed in both breast cancer tissue and whole blood. As a preliminary assessment, our findings indicate that combined levels of MIAT, FOXO3a, and miR29a-3p may be considered as potential diagnostic biomarkers for breast cancer.
CUBCO+: Prediction of Protein Complexes Based on Min-cut Network Partitioning Into Biclique Spanned Subgraphs
Authors: Omranian Sara , Nikoloski Zoran 
Published: March 2022

Abstract
High-throughput proteomics approaches have resulted in large-scale protein-protein interaction (PPI) networks that have been employed for the prediction of protein complexes. However, PPI networks contain false-positive as well as false-negative PPIs that affect the protein complex prediction algorithms. To address this issue, here we propose an algorithm called CUBCO+ that: (1) employs GO semantic similarity to retain only biologically relevant interactions with high similarity score, (2) based on link prediction approaches, scores the false-negative edges, and (3) incorporates the resulting scores to predict protein complexes. Through comprehensive analyses with PPIs from Escherichia coli, Saccharomyces cerevisiae, and Homo sapiens, we show that CUBCO+ performs as well as the approaches that predict protein complexes based on recently introduced graph partitions into biclique spanned subgraphs and outperforms the other state-of-the-art approaches. Moreover, we illustrate that in combination with GO semantic similarity, CUBCO+ enables us to predict more accurate protein complexes in ~36% of the cases in comparison to CUBCO as its predecessor.
Myocardial Triglyceride Content and Late Gadolinium Enhancement in Hypertrophic Cardiomyopathy with Asymmetric Septal Hypertrophy
Authors: Sai Eiryu , Hiki Makoto , Yokoyama Takayuki , Sato Shuji , Aikawa Tatsuro , Kudo Ayako , Kawaguchi Yuko , Miyazaki Tetsuro , Fujmoto Shinichiro , Shimada Kazunori , Aoki Shigeki , Daida Hiroyuki , Minamino Tohru 
Published: March 2022

Abstract
Asymmetric septal hypertrophy (ASH) is the most common phenotype of hypertrophic cardiomyopathy (HCM). However, the difference between myocardial metabolism and myocardial features such as myocardial fibrosis and myofiber disarray in HCM remains unclear. Cardiac magnetic resonance (CMR) and proton magnetic resonance spectroscopy (1H-MRS) were performed on 16 patients diagnosed as HCM with ASH to assess the differences in myocardial features and morphology. There were 7 patients who had LGE in ASH localized with the voxel of 1H-MRS. The LV ejection fraction was significantly lower in the LGE (+) group than in the LGE (−) group (64.8% ± 4.9% vs. 72.1% ± 3.8%, P = 0.004). LV end-systolic volume was significantly greater in the LGE (+) group than in the LGE (−) group (51 ± 11 ml vs. 33 ± 12 ml, P = 0.007). A significant difference in MTG content was observed between patients in the LGE (+) group and in the LGE (−) group (0.58% ± 0.22% vs 1.29% ± 0.73%, P = 0.03). MTG content was significantly associated with LV ejection fraction, LV end systolic volume, and LV mass. In multivariate analyses, LV ejection fraction had an independent association with MTG content (P = 0.01) Our data suggest that the metabolism of the septum in HCM with ASH may be different based on the absence or presence of LGE and that measurement of MTG content by 1H-MRS may be useful to evaluate the characteristics of myocardial metabolic changes caused by the LV remodeling process in HCM. (248 words)
Development and Initial Validation of a Frailty Score for Patients with Pediatric and Congenital Heart Disease
Authors: Studyvin Sarah , Birnbaum Brian F. , Staggs Vincent S. , Gross-Toalson Jami , Shirali Girish , Panchangam Chaitanya , White David A. 
Published: March 2022

Abstract
Frailty is a multi-dimensional clinical syndrome that is associated with increased morbidity and mortality and decreased quality of life. Children/adolescents with heart disease (HD) perform significantly worse for each frailty domain compared to non-HD peers. Our study aimed to create a composite frailty score (CFS) that can be applied to children/adolescents with HD and evaluate associations between the CFS and outcomes. Children and adolescents (n = 30) with HD (73% single ventricle, 20% heart failure, 7% pulmonary hypertension) were recruited from 2016 to 2017 (baseline). Five frailty domains were assessed at baseline using measures validated for pediatrics: 1) Slowness: 6-minute walk test; 2) Weakness: handgrip strength; 3) Fatigue: PedsQL Multi-dimensional Fatigue Scale; 4) Body composition: triceps skinfold thickness; 5) Physical activity questionnaire. Frailty points per domain (range = 0–5) were assigned based on z-scores or raw questionnaire scores and summed to produce a CFS (0 = least frail; 25 = most frail). Nonparametric bootstrapping was used to identify correlations between CFS and cross-sectional change in outcomes over 2.2 ± 0.2 years. The mean CFS was 12.5 ± 3.5. In cross-sectional analyses of baseline data, correlations (|r|≥0.25) were observed between CFS and NYHA class, the number of ancillary specialists, total prescribed medications, heart failure medications/day, exercise test derived chronotropic index and percent predicted VO2peak, and between child and parent-proxy PEDsQL. At follow-up, CFS was correlated with an increase in the number of heart failure medications (r = 0.31). CFS was associated with cross-sectional outcomes in youth with heart disease. Longitudinal analyses were limited by small sample sizes due to loss to follow-up.
Clinical and microbiological characteristics of and risk factors for bloodstream infections among patients with extracorporeal membrane oxygenation: A single-center retrospective cohort study
Authors: Lee Eun Hwa , Lee Ki Hyun , Lee Se Ju , Kim Jinnam , Baek Yae Jee , Ahn Jin Young , Jeong Su Jin , Ku Nam Su , Choi Jun Yong , Yeom Joon-Sup , Song Young Goo , Kim Jung Ho 
Published: March 2022

Abstract
Background Extracorporeal membrane oxygenation (ECMO) provides hemodynamic and oxygenation support to critically ill patients. Due to multiple catheter cannulations, patients on ECMO are vulnerable to bloodstream infections (BSIs). We aimed to investigate the incidence, clinical characteristics, risk factors, and microorganisms associated with BSIs during ECMO. MethodsThis single-center retrospective cohort study was conducted between January 2015 and May 2021. Patients aged 18 years or older with an ECMO duration of >48 hours for cardiogenic or respiratory support were included in the study. Patients who developed bacteremia or candidemia from 12 h after ECMO cannulation to 7 days after de-cannulation were included. The clinical factors between non-BSI and BSI were compared, along with an analysis of the risk factors associated with BSI during ECMO. Results A total of 480 patients underwent ECMO for cardiogenic shock (n=267, 55.6%) or respiratory failure (n=213, 44.4%) during the study period. The incidence was 20.0 episodes per 1,000 ECMO-days. Approximately 20.2% (97/480) and 5.4% (26/480) of the patients developed bacteremia and candidemia, respectively. The median numbers of days of BSI development were 8.00 days for bacteremia and 11.0 days for candidemia. The most common pathogens were methicillin-resistant coagulase-negative staphylococci (n=24), followed by vancomycin-resistant Enterococcus (n=21). Multivariable logistic analysis demonstrated that hemodialysis (odds ratio [OR]=2.647, p<0.001), veno-arterial-venous mode (OR=1.911, p=0.030), and total ECMO duration (OR=1.030, p=0.007) were significant risk factors for bacteremia. The total ECMO duration was the only risk factor associated with candidemia (OR=1.035, p=0.010). The mortality rate was significantly higher in the bacteremia (57.7 %) and candidemia (69.2 %) groups than that in the non-BSI group (43.6%). ConclusionBSI is a common complication of patients receiving ECMO support and is associated with poor clinical outcomes. Determining the type of frequently isolated organisms and the median onset time of BSI would help in the selection of appropriate prophylactic antibiotics or antifungal agents.
Mean systemic filling pressure indicates fluid responsiveness and anesthesia-induced "unstressed" blood volume
Authors: Hahn Robert G. , He Rui , Li Yuhong 
Published: March 2022

Abstract
Background. The mean systemic filling pressure (Pms) play a central role for the understanding of the circulation. We studied whether the Pms indicates fluid responsiveness before and after induction of general anesthesia and whether the Pms response to bolus infusions of fluid indicates an anesthesia-induced increase of the "unstressed" blood volume. Methods. An analog to Pms based on cardiac output, the mean arterial pressure, and central venous pressure (Pmsa) was calculated in 86 patients before induction of general anesthesia and before each of 3 successive bolus infusions of 3 ml/kg of colloid fluid. An increase in stroke volume of ≥ 10% from a bolus infusion indicated fluid responsiveness. Changes in blood volume were estimated from anthropometric data and the hemodilution. Results. Pmsa was lower in fluid responders than in non-responders before induction (13.2 ± 2.2 vs. 14.7 ± 2.7 mmHg; mean ± SD, P< 0.01) and after induction of general anesthesia (11.4 ± 2.1 vs. 12.8 ± 2.1 mmHg; P< 0.006). Changes in Pmsa resulting from the infusions did not differ depending on the stroke volume response. Receiver operator characteristic curves showed an average area under the curve of 0.70. The decrease in Pmsa due to the anesthesia-induced increase of the vascular compliance was fully compensated by the colloid fluid. A linear correlation between Pmsa and the volume changes suggested that the anesthesia increased the "unstressed" blood volume by 1.2 L. Conclusions. Pmsa was lower in fluid responders than in non-responders. General anesthesia increased the need for blood volume by 1.2 L.
Molecular Analysis of SMN2, NAIP and GTF2H2 Gene Deletions and Relation with Clinical Subtypes of Spinal Muscular Atrophy
Authors: Karasu Nilgun , Acer Hamit , Akalin Hilal , Demir Mikail , Sahin Izem Olcay , Gokce Nuriye , Gulec Ayten , Ciplakligil Asli , Sarilar Ayse Caglar , Cuce Isa , Gumus Hakan , Per Huseyin , Canpolat Mehmet , Dundar Munis 
Published: March 2022

Abstract
SMA (spinal muscular atrophy) is an autosomal recessive neuromuscular disease that causes muscle atrophy and weakness. SMA is diagnosed by homozygous deletion in exon 7 of the SMN1 gene. However, mutations in other genes in the SMA region may contribute to the disease. These include SMN2, which is a pseudogene of SMN1, as well as NAIP and GTF2H2. Within the scope of our study, 58 SMA patients and 40 healthy controls were analyzed in 2018–2021. SMN1 and SMN2 copy numbers were retrospectively included in the study. NAIP gene analyzes were performed by multiplex PCR method and GTF2H2 analyzes were performed by RFLP method respectively. We detected a significant correlation between clinical subtypes (type 2 and type 3) and ambulation status (p = 0.003) and HFMSE scores (p = 0.0063) of 27 pediatric SMA patients compared with separately. Highly differences were determined between SMN2 copy numbers and the SMA subtypes (p = 0.00001). Also, the NAIP gene (p = 0,0095) and the GTF2H2 gene (p = 0,0049) revealed a significant difference between the healthy subjects and SMA subjects, whereas in the SMA subtypes indicated no significant difference. Our investigation is the first to examine the relationship between SMA clinical severity and SMN locus genes in the Turkish population. This small-scale study may be regarded as a pilot study, and it may pave the way for future research to better understand the molecular pathophysiology of SMA disease.