bluuebunny/crossref_metadata_2025_split huggingface dataset:
Linking the Jehol Biota Evolution to the Early Cretaceous Volcanism During the North China Craton Destruction: Insights From F, Cl, S, and P
Authors: Xu Qi‐Hu , Wang Lu , Liu Jia , Deloule Etienne , Hanski Eero , Gu Xiao‐Yan , Chen Huan , Xia Qun‐Ke 
Published: June 2022

AbstractThe Early Cretaceous Jehol Biota evolution has remarkable spatiotemporal correlation with the destruction of the North China craton though the coupling mechanism remains enigmatic. The craton destruction was accompanied by intense magmatic activity and the released volatiles and nutrients might have had climatic and environmental impacts on the biotic evolution. In this study, we investigated the mentioned hypothetical causal link by determining concentrations and total emissions of volatile elements (S, F, Cl) and bulk‐rock P contents of volcanic rocks that were erupted during the pre‐flourishing, flourishing and post‐flourishing stages of the Jehol Biota. Our results show that the volcanism near the flourishing stage has lower S (1,083–2,370 ppm), Cl (1,277–5,608 ppm) and higher P2O5 contents (0.48–0.84 wt.%) than that in the non‐flourishing stages with S of 1,991–3,288 ppm, Cl of 7,915–12,315 ppm and P2O5 of 0.17–0.23 wt.%. Fluorine contents in the three stages vary from 893 to 3,746 ppm. The total volatile emissions are minor in the flourishing stage (1–14 Gt S, 0.6–10 Gt Cl, 0.6–9 Gt F) but elevated in the non‐flourishing stages (2–766 Gt S, 4–1,168 Gt Cl, 1–175 Gt F). Our data suggest that regional climatic and environmental impacts of volcanism in the non‐flourishing stages probably hindered the species diversification. The high P flux released from lithospheric mantle‐derived lavas during the peak time of craton destruction might have enhanced primary productivity and contributed to the flourishing of the Jehol Biota. Our study provides insights into the relationship between the biosphere and deep geodynamic processes driven by volcanism.
An mm-sized biomimetic directional microphone array for sound source localization in three dimensions
Authors: Rahaman Ashiqur , Kim Byungki 
Published: June 2022

AbstractFlyOrmia ochraceaears have been well-studied and mimicked to achieve subwavelength directional sensing, but their efficacy in sound source localization in three dimensions, utilizing sound from theX-,Y-, andZ-axes, has been less explored. This paper focuses on a mm-sized array of threeOrmia ochraceaear-inspired piezoelectric MEMS directional microphones, where their in-plane directionality is considered a cue to demonstrate sound source localization in three dimensions. In the array, biomimetic MEMS directional microphones are positioned in a 120° angular rotation; as a result, six diaphragms out of three directional microphones keep a normal-axis relative to the sound source at six different angles in the azimuth plane starting from 0° to 360° in intervals of ±30°. In addition, the cosine-dependent horizontal component of the applied sound gives cues forZ-axis directional sensing. The whole array is first analytically simulated and then experimentally measured in an anechoic chamber. Both results are found to be compliant, and the angular resolution of sound source localization in three dimensions is found to be ±2° at the normal axis. The resolution at the azimuth plane is found to be ±1.28°, and the same array shows a ± 4.28° resolution when sound is varied from the elevation plane. Looking at the scope within this area combined with the presented results, this work provides a clear understanding of sound source localization in three dimensions.
Distributed genetic architecture across the hippocampal formation implies common neuropathology across brain disorders
Authors: Bahrami Shahram , Nordengen Kaja , Shadrin Alexey A. , Frei Oleksandr , van der Meer Dennis , Dale Anders M. , Westlye Lars T. , Andreassen Ole A. , Kaufmann Tobias 
Published: June 2022

AbstractDespite its major role in complex human functions across the lifespan, most notably navigation, learning and memory, much of the genetic architecture of the hippocampal formation is currently unexplored. Here, through multivariate genome-wide association analysis in volumetric data from 35,411 white British individuals, we reveal 177 unique genetic loci with distributed associations across the hippocampal formation. We identify genetic overlap with eight brain disorders with typical onset at different stages of life, where common genes suggest partly age- and disorder-independent mechanisms underlying hippocampal pathology.
Anethole rich Clausena heptaphylla (Roxb.) Wight & Arn., essential oil pharmacology and genotoxic efficiencies
Authors: Lal Mohan , Begum Twahira , Gogoi Roktim , Sarma Neelav , Munda Sunita , Pandey Sudin Kumar , Baruah Joyashree , Tamang Raghu , Saikia Samarjit 
Published: June 2022

AbstractAnethole, a widely used industrial flavoring agent is majorly sourced from anise and star anise. The present study is aimed to the in-depth pharmacological analysis i.e. anti-diabetic, skin whitening, neurodegenerative disorder inhibitory activities of anethole-rich Clausena heptaphylla leaf essential oil (ARCHEO) (88.59%) as revealed by the Gas Chromatography/Mass Spectrometry (GC/MS) analysis and further confirmed by proton nuclear magnetic resonance 1H-NMR as well as to compare with standard compound anethole. ARCHEO (ABTS EC50 6.97 ± 0.004 µg/mL; Protease assay 4.51 ± 0.004 µg/mL) outperformed the standard compound anethole (ABTS EC50 9.48 ± 0.048 µg/mL; Protease assay EC50 22.64 ± 0.016 µg/mL) in antioxidant and anti-inflammatory experiments. ARCHEO was also shown to be more effective than the reference compound anethole in terms of anti-diabetic activity (EC50 22.35 ± 0.121 µg/mL), tyrosinase inhibitory activity (EC50 16.45 ± 0.012 µg/mL), and anti-cholinesterase activity (EC50 22.32 ± 0.016 µg/mL). However, ARCHEO exhibited lower antimicrobial activity towards all the tested microbes compared to standard compound anethole and as for the MIC, ARCHEO was effective only towards Salmonella typhimurium (60 µg/mL), Streptococcus mutans (20 µg/mL), and Aspergillus fumigatus (75 µg/mL). ARCHEO (11.11%) and anethole (12.33%) showed no genotoxic effect based on Allium cepa assay mitotic index value. Thus, ARCHEO could be a commercially viable and widely available cheaper source of anethole, which has buoyant demand in the field of food flavoring, fragrance, and pharmaceutical industries.
Identification of a novel prognostic signature for HCC and analysis of costimulatory molecule-related lncRNA AC099850.3
Authors: Wang Qi , Fang Qiong , Huang Yanping , Zhou Jin , Liu Meimei 
Published: June 2022

AbstractCostimulatory molecules are involved in initiation of anti-tumor immune responses while long non‐coding RNAs (lncRNAs) regulate the development of various cancers. However, the roles of lncRNA in hepatocellular carcinoma (HCC) have not been fully established. In this study, we aimed at identifying lncRNAs-related costimulatory molecules in HCC and to construct a prognostic signature for predicting the clinical outcomes for HCC patients. Data were downloaded from The Cancer Genome Atlas database for bioinformatics analyses. Costimulatory molecules were obtained from published literature. The R software, SPSS, and GraphPad Prism were used for statistical analyses. A risk model that is based on five costimulatory molecule-related lncRNAs was constructed using lasso and Cox regression analyses. Multivariate regression analysis revealed that the risk score could predict the prognostic outcomes for HCC. Samples in high- and low-risk groups exhibited significant differences in gene set enrichment and immune infiltration levels. Through colony formation and CCK8 assays, we found that AC099850.3 was strongly associated with HCC cell proliferation. We identified and validated a novel costimulatory molecule-related survival model. In addition, AC099850.3 was found to be closely associated with clinical stages and proliferation of HCC cells, making it a potential target for HCC treatment.
Identification of aquifer pollution’s point sources with the reciprocity principle
Authors: Bouhlila Rachida , Hariga Nejla T. 
Published: June 2022

AbstractThe principle of reciprocity, called Maxwell–Betti theorem, initially used in mechanics in an elastic structure, establishes a relation of equality between two distinct strains under different loads. In this paper, we extend and apply this principle to flow and solute transport equations in porous media, in order to perform the pollution sources identification in aquifers. We developed general 2D expressions of the reciprocity principle for transient transport problems. This model leads to a linear equations set, with point sources coordinates, concentrations and associated water fluxes as unknowns The proposed model is then applied to the Rocky Mountain Arsenal aquifer (Konikow in Modeling Chloride Movement in the Alluvial Aquifer at the Rocky Mountain Arsenal, Colorado. Technical Report Water-Supply Paper 2044, USGS, 1979), where polluted water is injected into a well in the domain. The used inverse technique successfully recovered the position and the pollutant concentration in addition to the associated water flux. In addition, we developed and implemented the inverse method for different knowledge levels of the degrees of the aquifer contamination, i.e. more or less data available in the field. Multiple pollution point sources and noisy data situations are also developed and tested with high efficiency. The proposed method would be easy and useful to be implemented in the modeling software now widely used by researchers and groundwater managers. It can thus be applied in real case studies, to help authorities and regulators to efficiently identify the polluters and the contamination process, i.e. its location, onset, duration and the associated mass and water fluxes.
Prevention of lymphocele or seroma after mastectomy and axillary lymphadenectomy for breast cancer: systematic review and meta-analysis
Authors: Adrien Crestani , Katia Mahiou , Marie-Lucile Bodet , Alice Roosen , Claire Bonneau , Roman Rouzier 
Published: June 2022

AbstractSeroma or lymphocele remains the most common complication after mastectomy and lymphadenectomy for breast cancer. Many different techniques are available to prevent this complication: wound drainage, reduction of the dead space by flap fixation, use of various types of energy, external compression dressings, shoulder immobilization or physical activity, as well as numerous drugs and glues. We searched MEDLINE, clinicaltrials.gov, Cochrane Library, and Web of Science databases for publications addressing the issue of prevention of lymphocele or seroma after mastectomy and axillary lymphadenectomy. Quality was assessed using Hawker’s quality assessment tool. Incidence of seroma or lymphocele were collected. Fifteen randomized controlled trials including a total of 1766 patients undergoing radical mastectomy and axillary lymphadenectomy for breast cancer were retrieved. The incidence of lymphocele or seroma in the study population was 24.2% (411/1698): 25.2% (232/920) in the test groups and 23.0% (179/778) in the control groups. Neither modification of surgical technique (RR 0.86; 95% CI [0.72, 1.03]) nor application of a medical treatment (RR 0.96; 95% CI [0.72, 1.29]) was effective in preventing lymphocele. On the contrary, decreasing the drainage time increased the risk of lymphocele (RR 1.88; 95% CI [1.43, 2.48). There was no publication bias but the studies were of medium to low quality. To conclude, despite the heterogeneity of study designs, drainage appears to be the most effective technique, although the overall quality of the data is low.
Immuno-reactive cancer organoid model to assess effects of the microbiome on cancer immunotherapy
Authors: Shelkey Ethan , Oommen David , Stirling Elizabeth R. , Soto-Pantoja David R. , Cook Katherine L. , Lu Yong , Votanopoulos Konstantinos I. , Soker Shay 
Published: June 2022

AbstractImmune checkpoint blockade (ICB) therapy has demonstrated good efficacy in many cancer types. In cancers such as non-resectable advanced or metastatic triple-negative breast cancer (TNBC), it has recently been approved as a promising treatment. However, clinical data shows overall response rates (ORRs) from ~ 3–40% in breast cancer patients, depending on subtype, previous treatments, and mutation status. Composition of the host-microbiome has a significant role in cancer development and therapeutic responsiveness. Some bacterial families are conducive to oncogenesis and progression, while others aid innate and therapeutically induced anti-tumor immunity. Modeling microbiome effects on anti-tumor immunity in ex vivo systems is challenging, forcing the use of in vivo models, making it difficult to dissect direct effects on immune cells from combined effects on tumor and immune cells. We developed a novel immune-enhanced tumor organoid (iTO) system to study factors affecting ICB response. Using the 4T1 TNBC murine cell line and matched splenocytes, we demonstrated ICB-induced response. Further administration of bacterial-derived metabolites from species found in the immunomodulatory host-microbiome significantly increased ICB-induced apoptosis of tumor cells and altered immune cell receptor expression. These outcomes represent a method to isolate individual factors that alter ICB response and streamline the study of microbiome effects on ICB efficacy.
Compact resonant systems for perfect and broadband sound absorption in wide waveguides in transmission problems
Authors: Boulvert Jean , Gabard Gwénaël , Romero-García Vicente , Groby Jean-Philippe 
Published: June 2022

AbstractThis work deals with wave absorption in reciprocal asymmetric scattering problem by addressing the acoustic problem of compact absorbers for perfect unidirectional absorption, flush mounted to the walls of wide ducts. These absorbers are composed of several side-by-side resonators that are usually of different geometry and thus detuned to yield an asymmetric acoustic response. A simple lumped-element model analysis is performed to link the dependence of the optimal resonators surface impedance, resonance frequency, and losses to the duct cross-sectional area and resonator spacing. This analysis unifies those of several specific configurations into a unique problem. In addition, the impact of the potential evanescent coupling between the resonators, which is usually neglected, is carefully studied. This coupling can have a strong impact especially on the behavior of compact absorbers lining wide ducts. To reduce the evanescent coupling, the resonators should be relatively small and therefore their resonances should be damped, and not arranged by order of increasing or decreasing resonant frequency. Finally, such an absorber is designed and optimized for perfect unidirectional absorption to prove the relevance of the analysis. The absorber is 30 cm long and 5 cm thick and covers a single side of a 14.8 × 15 cm2 rectangular duct. A mean absorption coefficient of 99% is obtained experimentally between 700 and 800 Hz.
Identification of useful genes from multiple microarrays for ulcerative colitis diagnosis based on machine learning methods
Authors: Zhang Lin , Mao Rui , Lau Chung Tai , Chung Wai Chak , Chan Jacky C. P. , Liang Feng , Zhao Chenchen , Zhang Xuan , Bian Zhaoxiang 
Published: June 2022

AbstractUlcerative colitis (UC) is a chronic relapsing inflammatory bowel disease with an increasing incidence and prevalence worldwide. The diagnosis for UC mainly relies on clinical symptoms and laboratory examinations. As some previous studies have revealed that there is an association between gene expression signature and disease severity, we thereby aim to assess whether genes can help to diagnose UC and predict its correlation with immune regulation. A total of ten eligible microarrays (including 387 UC patients and 139 healthy subjects) were included in this study, specifically with six microarrays (GSE48634, GSE6731, GSE114527, GSE13367, GSE36807, and GSE3629) in the training group and four microarrays (GSE53306, GSE87473, GSE74265, and GSE96665) in the testing group. After the data processing, we found 87 differently expressed genes. Furthermore, a total of six machine learning methods, including support vector machine, least absolute shrinkage and selection operator, random forest, gradient boosting machine, principal component analysis, and neural network were adopted to identify potentially useful genes. The synthetic minority oversampling (SMOTE) was used to adjust the imbalanced sample size for two groups (if any). Consequently, six genes were selected for model establishment. According to the receiver operating characteristic, two genes of OLFM4 and C4BPB were finally identified. The average values of area under curve for these two genes are higher than 0.8, either in the original datasets or SMOTE-adjusted datasets. Besides, these two genes also significantly correlated to six immune cells, namely Macrophages M1, Macrophages M2, Mast cells activated, Mast cells resting, Monocytes, and NK cells activated (P  <  0.05). OLFM4 and C4BPB may be conducive to identifying patients with UC. Further verification studies could be conducted.
Decoronation-induced infected alveolar socket defect rat model for ridge preservation
Authors: Fang Chih-Hsiang , Lin Hung-Ying , Sun Chung-Kai , Lin Yi-Wen , Hung Min-Chih , Li Ching-Hung , Lin I-Ping , Chang Hung-Chen , Sun Jui-Sheng , Chang Jenny Zwei-Chieng 
Published: June 2022

AbstractCurrent rat alveolar ridge preservation models have not been well standardized. In this study, we proposed decoronation-induced infected alveolar socket model of rat. The bilateral maxillary first molars (M1) of twenty-four rats were decoronized or extracted. After 2, 6, 10, and 14 weeks, bone and soft tissue changes at M1 and periodontal conditions of maxillary second (M2) and third molars (M3) were evaluated by micro-computed tomography and histological analysis. Additional eighteen rats with standardized size defects were grafted with Bio-Oss Collagen to compare with unmanipulated contralateral side. Decoronation preserved greater bone and soft tissue dimensions at M1, provided larger three-dimensional (3D) bone contour volume, but also promoted periodontal breakdown of M2 Histological results showed intense inflammatory cell infiltrations and severe bone resorption within M1 socket and at mesial aspect of M2. The critical dimensions to accommodate largest standardized defect at M1 were 2.2–2.3 mm at vertical bone height and 2.8–3.2 mm at alveolar crestal width. Bio-Oss Collagen could not fully preserve buccal or palatal bone height but could be beneficial in preserving ridge width in large alveolar defects. Collectively, if periodontally-involved alveolar bone defect is preferred, we suggest extracting M1 roots 6 weeks after decoronation to allow periodontitis to occur at M2. If standardized critical dimension defect is preferred, we suggest extracting M1 roots 2 weeks after decoronation, and creating defect in the middle of M1 site with size no larger than 2.7 mm diameter to its full depth.
A novel photosynthetic biologic topical gel for enhanced localized hyperoxygenation augments wound healing in peripheral artery disease
Authors: Zhu Yuanjia , Jung Jinsuh , Anilkumar Shreya , Ethiraj Sidarth , Madira Sarah , Tran Nicholas A. , Mullis Danielle M. , Casey Kerriann M. , Walsh Sabrina K. , Stark Charles J. , Venkatesh Akshay , Boakye Alexander , Wang Hanjay , Woo Y. Joseph 
Published: June 2022

AbstractPeripheral artery disease and the associated ischemic wounds are substantial causes of global morbidity and mortality, affecting over 200 million people worldwide. Although advancements have been made in preventive, pharmacologic, and surgical strategies to treat this disease, ischemic wounds, a consequence of end-stage peripheral artery disease, remain a significant clinical and economic challenge. Synechococcus elongatus is a cyanobacterium that grows photoautotrophically and converts carbon dioxide and water into oxygen. We present a novel topical biologic gel containing S. elongatus that provides oxygen via photosynthesis to augment wound healing by rescuing ischemic tissues caused by peripheral artery disease. By using light rather than blood as a source of energy, our novel topical therapy significantly accelerated wound healing in two rodent ischemic wound models. This novel topical gel can be directly translated to clinical practice by using a localized, portable light source without interfering with patients’ daily activities, demonstrating potential to generate a paradigm shift in treating ischemic wounds from peripheral artery disease. Its novelty, low production cost, and ease of clinical translatability can potentially impact the clinical care for millions of patients suffering from peripheral arterial disease.
Melting phase relations in Fe–Si–H at high pressure and implications for Earth’s inner core crystallization
Authors: Hikosaka Koutaro , Tagawa Shoh , Hirose Kei , Okuda Yoshiyuki , Oka Kenta , Umemoto Koichiro , Ohishi Yasuo 
Published: June 2022

AbstractHydrogen could be an important light element in planetary cores, but its effect on phase diagrams of iron alloys is not well known because the solubility of H in Fe is minimal at ambient pressure and high-pressure experiments on H-bearing systems have been challenging. Considering that silicon can be another major light element in planetary cores, here we performed melting experiments on the Fe–Si–H system at ~ 50 GPa and obtained the ternary liquidus phase relations and the solid/liquid partition coefficient,Dof Si and H based onin-situhigh-pressure X-ray diffraction measurements andex-situchemical and textural characterizations on recovered samples. Liquid crystallized hexagonal close-packed (hcp) (Fe0.93Si0.07)H0.25, which explains the observed density and velocities of the Earth’s solid inner core. The relatively highDSi = 0.94(4) andDH = 0.70(12) suggest that in addition to Si and H, the liquid outer core includes other light elements such as O, which is least partitioned into solid Fe and can thus explain the density difference between the outer and inner core. H and O, as well as Si, are likely to be major core light elements, supporting the sequestration of a large amount of water in the Earth’s core.