bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicting habitat suitability and niche dynamics of Dactylorhiza hatagirea and Rheum webbianum in the Himalaya under projected climate change
Authors: Wani Ishfaq Ahmad , Khan Sajid , Verma Susheel , Al-Misned Fahad A. , Shafik Hesham M. , El-Serehy Hamed A. 
Published: August 2022

AbstractIn the era of anthropocene, global warming tends to alter the distribution range of the plant species. Highly fragile to such changes are the species that are endemic, inhabit higher elevations and show narrow distribution ranges. Predicting and plotting the appropriate suitable habitats and keeping knowledge of how climate change will affect future distribution become imperative for designing effective conservation strategies. In the current study we have used BIOMOD ensemble forecasting to study the current and predict the future potential distribution of Dactylorhiza hatagirea and Rheum webbianum and describe their niche dynamics in Himalayan biodiversity hotspots under climate change scenarios using ecospat R package. Results reveal sufficient internal evaluation metrics with area under curve (AUC) and true skill statistic (TSS) values greater than 0.8 i.e. 0.93 and 0.98 and 0.82 and 0.90 for D. hatageria and R. webbianum respectively, which signifies robustness of the model. Among different bioclimatic variables, bio_1, bio_3, bio_8, bio_14 and bio_15 were the most influential, showing greater impact on the potential distribution of these plant species. Range change analysis showed that both the studied species will show significant contraction of their suitable habitats under future climatic scenarios. Representative Concentration Pathway (RCP) 8.5 for the year 2070, indicate that the suitable habitats could be reduced by about 51.41% and 70.57% for D. hatagirea and R. webbianum respectively. The results of the niche comparisons between the current and future climatic scenarios showed moderate level of niche overlap for all the pairs with D. hatageria showing 61% overlap for current vs. RCP4.5 2050 and R. webbianum reflects 68% overlap for current vs. RCP4.5 2050. Furthermore, the PCA analysis revealed that climatic conditions for both the species vary significantly between current and future scenarios. The similarity and equivalence test showed that the niche between present and future climate change scenarios is comparable but not identical. From the current study we concluded that the influence of climate change on the habitat distribution of these plant species in the Himalayan biodiversity hotspots can be considered very severe. Drastic reduction in overall habitat suitability poses a high risk of species extinction and thereby threatens to alter the functions and services of these fragile ecosystems. Present results can be used by conservationists for mitigating the biodiversity decline and exploring undocumented populations on one hand and by policymakers in implementing the policy of conservation of species by launching species recovery programmes in future on the other. The outcomes of this study can contribute substantially to understand the consequences of climate change in the Himalayan biodiversity hotspots.
The E3 ubiquitin ligase HECTD1 contributes to cell proliferation through an effect on mitosis
Authors: Vaughan Natalie , Scholz Nico , Lindon Catherine , Licchesi Julien D. F. 
Published: August 2022

AbstractThe cell cycle is tightly regulated by protein phosphorylation and ubiquitylation events. During mitosis, the multi-subunit cullin-RING E3 ubiquitin ligase APC/c functions as a molecular switch which signals for one cell to divide into two daughter cells, through the ubiquitylation and proteasomal degradation of mitotic cyclins. The contributions of other E3 ligase families during cell cycle progression remain less well understood. Similarly, the roles of ubiquitin chain types beyond homotypic K48 chains in S-phase or branched K11/K48 chains during mitosis, also remain to be fully determined. Our recent findings that HECTD1 ubiquitin ligase activity assembles branched K29/K48 ubiquitin linkages prompted us to evaluate HECTD1 function during the cell cycle. We used transient knockdown and genetic knockout to show that HECTD1 depletion in HEK293T and HeLa cells decreases cell number and we established that this is mediated through loss of ubiquitin ligase activity. Interestingly, we found that HECTD1 depletion increases the proportion of cells with aligned chromosomes (Prometa/Metaphase) and we confirmed this molecularly using phospho-Histone H3 (Ser28) as a marker of mitosis. Time-lapse microscopy of NEBD to anaphase onset established that HECTD1-depleted cells take on average longer to go through mitosis. In line with this data, HECTD1 depletion reduced the activity of the Spindle Assembly Checkpoint, and BUB3, a component of the Mitosis Checkpoint Complex, was identified as novel HECTD1 interactor. BUB3, BUBR1 or MAD2 protein levels remained unchanged in HECTD1-depleted cells. Overall, this study reveals a novel putative role for HECTD1 during mitosis and warrants further work to elucidate the mechanisms involved.
Midguts of Culex pipiens L. (Diptera: Culicidae) as a potential source of raw milk contamination with pathogens
Authors: Adly Eslam , Hegazy Aml A. , Kamal Mahmoud , Abu-Hussien Samah H. 
Published: August 2022

AbstractDespite their importance, only few studies focused on the mosquitoes borne microbial diseases, especially bacterial and fungal diseases, their vectorial capacity toward microorganisms, and their important role in raw milk contamination with pathogens in some unsanitary dairy farms. In Egypt, whereCulex pipiensis the historical main vector of lymphatic filariasis, only few studies discussed the isolation of pathogens from the midguts of different stages ofC. pipiens. This study aims to isolate and identify the pathogenic symbiotic microorganisms inside the midgut of adult femaleC. pipiensas well as investigate its ability to transmit their midgut pathogens to raw milk. A total of 750 field strain C. pipiens larvae of the second and third larval instars were collected from ponds water around the livestock farms in Mariotteya, Giza, Egypt, for microbial pathogen isolation and identification. All collected larval instars were transported to the laboratory at the Research and Training Center on Vectors of Diseases (RTC), Ain Shams University, where they were maintained for further studies. Six groups ofC. pipienswere tested for the incidence of various pathogenic microorganisms in their midguts and their possibility to contaminate commercial sterilized milk. Traditional PCR assays and sequencing method detected and identified 16srRNA genes of the predominant hemolytic isolates from milk and midguts of femaleC. pipiens. The phylogenetic analyses of the obtained isolates were performed based on NCBI data. Three strains ofBacillus anthracisstrain CPMESA 2021,Staphylococcus warneristrain CPSAME 2021, andBacillus cereusstrain CPSEMA 2021, which represent most food pathogens, were found in the midguts ofC. pipiensand were submitted to the GenBank database with the accession numbers OK585071, OK576651, and OK585052, respectively. The isolation of these strains from mosquitoes raises contemporary issues concerning milk safety, such as bacterial isolates, the degree of the vectorial capacity of mosquitoes, milk production and processing conditions, and human pathogenicity. Such serious issues need further investigation.
Probing spin fluctuations of the quantum phase transition in Ce3Al by muon spin rotation
Authors: Pregelj Matej , Guguchia Zurab , de Weerd Marie-Cécile , Boulet Pascal , Vrtnik Stanislav , Dolinšek Janez 
Published: August 2022

AbstractWe report on the dynamics of a magnetic-field-driven antiferromagnetic-to-paramagnetic quantum phase transition in monocrystalline Ce3Al via transverse-field muon spin rotation (TF-µSR) experiments down to low temperature of $$\sim$$
∼
80 mK. The quantum phase transition is of a spin-flip type and takes place on the Ce–Al magnetic chains as a result of competition between the indirect exchange and the Zeeman interaction of the Ce moments with the external field, applied along the chain direction (also the direction of the antiferromagnetic axis). The Ce moments are not static at $$T \to$$

T
→

0, but fluctuate in their direction due to the Heisenberg uncertainty principle. Upon applying the magnetic field sweep, the fluctuations exhibit the largest amplitude at the quantum critical point, manifested in a maximum of the muon transverse relaxation rate at the critical field. The quantum nature of fluctuations observed in the TF-µSR experiments is reflected in the temperature independence of the average local magnetic field component along the external magnetic field at the muon stopping site(s) and the muon transverse relaxation rate within the investigated temperature range 1.5 K–80 mK. Quantum fluctuations are fast on the muon Larmor frequency scale, $$\tau_{0} <$$

τ
0

<

10–10 s.
Control of structural redundancy from the head to trunk in the human upright standing revealed using a data-driven approach
Authors: Tanaka Kazuya , Fujiki Soichiro , Atomi Tomoaki , Takano Wataru , Hasegawa Katsuya , Nagano Akinori , Shimizu Miho , Atomi Yoriko 
Published: August 2022

AbstractThe human being dynamically and highly controls the head–trunk with redundant mechanical structures to maintain a stable upright standing position that is inherently unstable. The posture control strategies are also affected by the differences in the conditions of sensory inputs. However, it is unclear how the head–trunk segmental properties are altered to respond to situations that require appropriate changes in standing posture control strategies. We used a data-driven approach to conduct a multipoint measurement of head–trunk sway control in a quiet standing position with differences in the conditions of sensory inputs. Healthy young subjects with 22 accelerometers attached to their backs were evaluated for head–trunk vibration during quiet standing under two conditions: one with open eyes and one with closed eyes. The synchronization of the acceleration and the instantaneous phase was then calculated. The results showed that the synchronization of acceleration and instantaneous phase varied depending on the visual condition, and there were some continuous coherent patterns in each condition. Findings were that the structural redundancy of the head–trunk, which is multi-segmental and has a high mass ratio in the whole body, must be adjusted adaptively according to the conditions to stabilize upright standing in human-specific bipeds.
Evaluating the efficiency of coarser to finer resolution multispectral satellites in mapping paddy rice fields using GEE implementation
Authors: Waleed Mirza , Mubeen Muhammad , Ahmad Ashfaq , Habib-ur-Rahman Muhammad , Amin Asad , Farid Hafiz Umar , Hussain Sajjad , Ali Mazhar , Qaisrani Saeed Ahmad , Nasim Wajid , Javeed Hafiz Muhammad Rashad , Masood Nasir , Aziz Tariq , Mansour Fatma , EL Sabagh Ayman 
Published: August 2022

AbstractTimely and accurate estimation of rice-growing areas and forecasting of production can provide crucial information for governments, planners, and decision-makers in formulating policies. While there exists studies focusing on paddy rice mapping, only few have compared multi-scale datasets performance in rice classification. Furthermore, rice mapping of large geographical areas with sufficient accuracy for planning purposes has been a challenge in Pakistan, but recent advancements in Google Earth Engine make it possible to analyze spatial and temporal variations within these areas. The study was carried out over southern Punjab (Pakistan)-a region with 380,400 hectares devoted to rice production in year 2020. Previous studies support the individual capabilities of Sentinel-2, Landsat-8, and Moderate Resolution Imaging Spectroradiometer (MODIS) for paddy rice classification. However, to our knowledge, no study has compared the efficiencies of these three datasets in rice crop classification. Thus, this study primarily focuses on comparing these satellites’ data by estimating their potential in rice crop classification using accuracy assessment methods and area estimation. The overall accuracies were found to be 96% for Sentinel-2, 91.7% for Landsat-8, and 82.6% for MODIS. The F1-Scores for derived rice class were 83.8%, 75.5%, and 65.5% for Sentinel-2, Landsat-8, and MODIS, respectively. The rice estimated area corresponded relatively well with the crop statistics report provided by the Department of Agriculture, Punjab, with a mean percentage difference of less than 20% for Sentinel-2 and MODIS and 33% for Landsat-8. The outcomes of this study highlight three points; (a) Rice mapping accuracy improves with increase in spatial resolution, (b) Sentinel-2 efficiently differentiated individual farm level paddy fields while Landsat-8 was not able to do so, and lastly (c) Increase in rice cultivated area was observed using satellite images compared to the government provided statistics.
Piezoelectric nanocomposite bioink and ultrasound stimulation modulate early skeletal myogenesis
Authors: Paci Claudia , Iberite Federica , Arrico Lorenzo , Vannozzi Lorenzo , Parlanti Paola , Gemmi Mauro , Ricotti Leonardo 
Published: August 2022

A cell-laden alginate/Pluronic-based bioink doped with BaTiO3 piezoelectric nanoparticles (BTNPs) was investigated. BTNPs promoted myogenic differentiation and the synergy with ultrasound boosted the expression of MYOD1, MYOG, and MYH2 genes.
Harnessing adult-plant resistance genes to deploy durable disease resistance in crops
Authors: Dinglasan Eric , Periyannan Sambasivam , Hickey Lee T. 
Published: August 2022

Abstract
Adult-plant resistance (APR) is a type of genetic resistance in cereals that is effective during the later growth stages and can protect plants from a range of disease-causing pathogens. Our understanding of the functions of APR-associated genes stems from the well-studied wheat-rust pathosystem. Genes conferring APR can offer pathogen-specific resistance or multi-pathogen resistance, whereby resistance is activated following a molecular recognition event. The breeding community prefers APR to other types of resistance because it offers broad-spectrum protection that has proven to be more durable. In practice, however, deployment of new cultivars incorporating APR is challenging because there is a lack of well-characterised APRs in elite germplasm and multiple loci must be combined to achieve high levels of resistance. Genebanks provide an excellent source of genetic diversity that can be used to diversify resistance factors, but introgression of novel alleles into elite germplasm is a lengthy and challenging process. To overcome this bottleneck, new tools in breeding for resistance must be integrated to fast-track the discovery, introgression and pyramiding of APR genes. This review highlights recent advances in understanding the functions of APR genes in the well-studied wheat-rust pathosystem, the opportunities to adopt APR genes in other crops and the technology that can speed up the utilisation of new sources of APR in genebank accessions.
The Acoustic Features and Didactic Function of Foreigner-Directed Speech: A Scoping Review
Authors: Piazza Giorgio , Martin Clara D. , Kalashnikova Marina 
Published: August 2022

Purpose:
This scoping review considers the acoustic features of a clear speech register directed to nonnative listeners known as foreigner-directed speech (FDS). We identify vowel hyperarticulation and low speech rate as the most representative acoustic features of FDS; other features, including wide pitch range and high intensity, are still under debate. We also discuss factors that may influence the outcomes and characteristics of FDS. We start by examining accommodation theories, outlining the reasons why FDS is likely to serve a didactic function by helping listeners acquire a second language (L2). We examine how this speech register adapts to listeners' identities and linguistic needs, suggesting that FDS also takes listeners' L2 proficiency into account. To confirm the didactic function of FDS, we compare it to other clear speech registers, specifically infant-directed speech and Lombard speech.

Conclusions:
Our review reveals that research has not yet established whether FDS succeeds as a didactic tool that supports L2 acquisition. Moreover, a complex set of factors determines specific realizations of FDS, which need further exploration. We conclude by summarizing open questions and indicating directions and recommendations for future research.
Supporting the Social–Emotional Well-Being of Elementary School Students Who Are Deaf and Hard of Hearing: A Pilot Study
Authors: Kishida Yuriko , Brennan-Jones Christopher G. , Runions Kevin , Vithiatharan Rena , Hancock Kirsten , Brown Michelle , Eikelboom Robert H. , Coffin Juli , Kickett-Tucker Cheryl , Li Ian W. , Epstein Melanie , Falconer Sarah E. , Cross Donna 
Published: August 2022

Purpose:Children who are Deaf and Hard of Hearing (DHH), their parents, Teachers of the Deaf, and other community stakeholders were involved in co-designing a web-based resource to support students' social–emotional well-being. The resource was designed to provide families and teachers with strategies to enhance the social and emotional well-being of Grade 4–6 students who are DHH. This study reports outcomes of a pilot study of the web-based resource intervention.Method:A pre–post pilot study was conducted to quantitatively examine reported anxiety, well-being, social relationships, school experience, student–teacher relationship, and parent and teacher self-efficacy. A total of 37 students, their parents (n= 37), and their classroom teachers (n= 40) participated in the intervention program and were provided access to the resource.Results:In total, 19 students, 22 parents, and 17 teachers completed both pre- and postsurvey measures. Pairedttests revealed that there was a statistically significant increase in parents' self-efficacy scores from pre- to posttest. Multivariate analysis of covariance revealed a significant association between parent use of the website and student-reported improved peer support and reduced school loneliness. No other statistically significant differences were found.Conclusions:The use of a web-based resource codeveloped with students who are DHH, their parents, and teachers could potentially be beneficial for the well-being of students who are DHH as well as parents' self-efficacy. Further research is needed to confirm the benefits.
Determinants and clinical significance of negative scintigraphic findings in primary hyperparathyroidism: a retrospective observational study
Authors: Neuberger Michael , Dropmann Jan Axl , Kleespies Axel , Fuerst Heinrich 
Published: August 2022

Abstract
Aim In patients with recurrent primary hyperparathyroidism (pHPT) or preceding thyroid operation, scintigraphic localization of the pathological parathyroid gland is sometimes unsuccessful. Reason for diagnostic failure, and its clinical relevance is poorly understood.
Methods This retrospective observational study in patients suffering from a pHPT evaluated independent predictors of a negative preoperative scintigraphy (SC) result, and its relevance for intraoperative outcome using logistic regression analysis.
Results Among 86 pHPT patients scheduled for parathyroid operation, 63 (73%) had a history of a preceding thyroid or parathyroid operation. Preoperative SC could not identify an adenoma in 30 patients (34.9%), and in 12 patients (14.0%), the surgeon was subsequently unable to localize abnormal parathyroid tissue. Preoperative parathyroid hormone concentration was the only significant independent predictor of a negative SC finding (non-linear and indirect association). Independent from surgical history, an unsuccessful intraoperative focus localization was exclusively predicted by preoperative ultrasonographic (US) and SC findings (OR per diagnostic category 2.98; 95%-CI 1.03–8.58, p=0.043, and OR 2.26; 95%-CI: 1.10–4.63, p=0.027, respectively). Compared to exclusive US, however, the combination of SC and US significantly increased the sensitivity and predictive power to identify patients at a high risk for a complicated surgical procedure.
Conclusion In patients before parathyroidectomy, a low preoperative parathyroid hormone concentration is significantly associated with a high likelihood for a negative SC finding. Combining US with SC before operation significantly increases the chance to identify patients prone to negative intraoperative findings.
Multi-Component Sequential Synthesis of Dihydroorotic Acid-Based Amphiphilic Molecules
Authors: Volonterio Alessandro , Sani Monica , Bellucci Maria Cristina 
Published: August 2022

AbstractAn efficient multicomponent sequential process, which occurs in mild condition has been exploited for the synthesis of systematically modified amphiphilic molecules where the cationic head is tethered to a lipophilic tail through a dihydroorotic acid linker. The process is operatively simple, high yielding, and flexible. Such a strategy could impact combinatorial synthesis of wide libraries of amphiphilic molecules to be tested as transfection agents and/or as antimicrobials.
The Use of Arteriovenous Bundle Interposition Grafts in Microsurgical Reconstruction: A Systematic Review of the Literature
Authors: Kareh Aurora M. , Tadisina Kashyap Komarraju , Chun Magnus , Kaswan Sumesh , Xu Kyle Y. 
Published: August 2022

AbstractMicrovascular reconstruction frequently requires anastomosis outside of the zone of injury for successful reconstruction. Multiple options exist for pedicle lengthening including vein grafts, arteriovenous loops, and arteriovenous bundle interposition grafts. The authors performed a systematic review of arteriovenous bundle interposition grafts to elucidate indications and outcomes of arteriovenous grafts in microvascular reconstruction. A systematic review of the literature was performed using targeted keywords. Data extraction was performed by two independent authors, and descriptive statistics were used to analyze pooled data. Forty-four patients underwent pedicle lengthening with an arteriovenous graft from the descending branch of the lateral circumflex femoral artery. Most common indications for flap reconstruction were malignancy (n = 12), trauma (n = 7), and diabetic ulceration (n = 4). The most commonly used free flap was the anterolateral thigh flap (n = 18). There were five complications, with one resulting in flap loss. Arteriovenous bundle interposition grafts are a viable option for pedicle lengthening when free flap distant anastomosis is required. The descending branch of the lateral circumflex femoral artery may be used for a variety of defects and can be used in conjunction with fasciocutaneous, osteocutaneous, muscle, and chimeric free flaps.