bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multidimensional analyses of the pathomechanism caused by the non-catalytic GNE variant, c.620A&gt;T, in patients with GNE myopathy
Authors: Yoshioka Wakako , Iida Aritoshi , Kyuto Sonehara , Yamamoto Kazuki , Oya Yasushi , Mori-Yoshimura Madoka , Kurashige Takashi , Okubo Mariko , Ogawa Megumu , Matsuda Fumihiko , Higasa Koichiro , Hayashi Shinichiro , Nakamura Harumasa , Sekijima Masakazu , Okada Yukinori , Noguchi Satoru , Nishino Ichizo 
Published: August 2022

Abstract
Background GNE myopathy is an autosomal recessive distal myopathy caused by biallelic variants in GNE, which encodes a protein involved in sialic acid biosynthesis. Compound heterozygosity of the second most frequent variant among Japanese patients with GNE myopathy, GNE c.620A>T, occurs in the expected number of patients; however, homozygotes for this variant are rare. The aim of this study was to elucidate the pathomechanism caused by c.620A>T.Methods Genome sequencing, identity-by-descent mapping, and RNA-seq analyses were conducted for GNE c.620A>T homozygotes. Blood and muscle sialylation levels were measured, and three-dimensional structural modeling of GNE oligomers was conducted using Alphafold2.Results The estimated frequency of the c.620A>T variant in the Japanese general population is 0.00161, equating to 238 homozygotes in total, but only three homozygotes with GNE myopathy have been identified. Identity-by-descent mapping indicated two distinct c.620A>T haplotypes, which were not correlated with patient clinical characteristics. Patients homozygous for c.620A>T had mildly decreased sialylation, and no additional pathogenic variants in GNE or abnormalities in transcript structure or expression of other genes related to sialic acid biosynthesis in skeletal muscle. Structural modeling of full-length GNE dimers revealed that the variant amino acid localized close to the monomer interface, but far from catalytic sites, suggesting functions in enzymatic product transfer between the epimerase and kinase domains on GNE oligomerization.Conclusion Homozygotes for c.620A>T rarely develop myopathy, while symptoms occur in compound heterozygotes, likely because of mildly decreased sialylation, due to partial defects in oligomerization and product trafficking by the mutated GNE protein.
Rational construction of Co-promoted 1T-MoS2 nanoflowers towards high-efficiency 4-nitrophenol reduction
Authors: Zhang Cen , Wang Li , Huang Xi , Bai Liang , Yu Qiyuan , Jiang Bin , Zheng Chenlu , Cao Jing 
Published: August 2022

Abstract
Developing efficient and cost-effective non-noble metal catalysts for the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) is of great importance. Herein, Co-promoted 1T-MoS2 nanoflowers were synthesized via a one-step hydrothermal method. The influence of Co content on the structure and catalytic performance of 1T-MoS2 was studied in detail. It was found the highest activity could be achieved due to the synergy effect of Co-Mo-S and CoS2 active phase. The reaction rate constant was as high as 0.908 min-1, and the catalyst exhibited excellent stability after recycling five times. We believe the understanding of the role of Co promoter would provide new insights for the rational design of highly efficient MoS2-based catalysts towards 4-NP reduction in wastewater.
Non-functional complications associated with radical hysterectomy
Authors: Alonso-Espías María , Alonso-García Marcos , García-Pineda Virginia , Gracia Myriam , Siegrist Jaime , Diestro María Dolores , Hernández Alicia , Zapardiel Ignacio 
Published: August 2022

Abstract

Purpose: Bladder dysfunction is the most frequent complication after radical hysterectomy. However, there are other relevant complications associated with cervical cancer surgery much less reported in literature. The aim of this study was to evaluate the frequency of non-functional complications associated with radical hysterectomy and to determine if there is any risk factor associated with their appearance. Methods: A retrospective study was conducted, including consecutive patients diagnosed with early-stage cervical cancer who underwent radical hysterectomy at La Paz University Hospital from January 2005 to December 2019. Data from intraoperative, short-term, and long-term complications were collected. A multivariate analysis was performed in order to identify potential predictors of surgical complications. Results: A total of 111 patients were included. Intraoperative complications occurred in 13(11.7%) women. Multivariate analysis showed a greater risk of intraoperative complications if microscopic parametrial involvement was present (OR=16.7; 95%CI 1.4-195). 41(36.9%) patients had any short-term postoperative complication, being urological the most frequent ones. Lymphedema was the most frequent long-term complication and occurred in 20(18%) patients. In short-term and long-term univariate analysis, TNM histological tumor stage was tended to be associated (p=0.07) to short-term postoperative complications, and FIGO stage was significatively associated (p=0.05) to long-term postoperative complications. Conclusion: Urological complications are the most frequent ones after radical hysterectomy. However, other less frequent complications such as genitourinary fistula or lymphedema are also important due to their impact on the quality of life of patients. We found that parametrial involvement in the postoperative pathological analysis was associated with higher intraoperative complications rate.
Nitrogen-doped graphene sheets as efficient nanofillers at ultra-low content for reinforcing mechanical and wear-resistant properties of acrylic polyurethane coatings
Authors: Xu Hui , Liu Danlian , Liang Lizhe , Tian Zhi Qun , Shen Pei Kang 
Published: August 2022

Abstract
The enhancement to the mechanical and wear-resistant properties of polymer coatings plays a vital role for their application in hostile serving environment and nanofiller is effective for this destination. Herein, we systematically investigate a new nanofiller, nitrogen-doped graphene sheets (NGS), which possess a multilayer sheet-like morphology and share a good compatibility with water. After the incorporation of NGS into a two-component waterborne polyurethane (WPU), the mechanical and wear-resistant properties of NGS/WPU composite coatings significantly improve and wear resistance behaves best at an ultra-low content, reaching up to 0.05 wt‰. Wherein, Young’s modulus is elevated by 52.67% and tensile strength is appreciably boosted by 58.87%. Simultaneously, apparent reduction of weight loss of 78.74% is observed in the abrasion testing, and the ductility of NGS/WPU composite films is reduced by 48.38%. These make it possible that an ultra-low content of nanofiller efficiently reinforces polymer-based composites to achieve a trade-off between mechanical properties. Moreover, the wear-resistance mechanism is investigated, and the interaction between NGS and WPU segments is explored to find the reason that the mechanical and wear-resistant properties of NGS/WPU composite coatings are improved at an ultra-low content.
Plasma GAS6 Predicts Mortality Risk in Acute Heart Failure Patients: Insights from the DRAGON-HF trial
Authors: Ma Teng , Huang Rongrong , Lv Yangbo , Liu Yifan , Pan Xin , Dong Jia , Gao Di , Wang Zeyu , Zhang Fenglei , Yan Chunxi , Ong Sang-Bing , Su Yang , Xu Dachun 
Published: August 2022

Abstract
Background: Growth arrest-specific 6 (GAS6) is a biomarker related to inflammation, fibrosis, as well as platelet function. Knockout of GAS6 in mice subjected to pressure overloading protects against cardiac hypertrophy and dysfunction. Nonetheless, the association between plasma GAS6 levels and acute heart failure (AHF) in patients is still unknown. Methods: We measured GAS6 concentrations in 1039 patients with AHF who were enrolled in the DRAGON-HF trial. We followed them and examine future clinical events. Results: In total, there were 195 primary endpoints of all-cause mortality and 135 secondary endpoints of cardiovascular death during the median follow-up period of 889 days. The higher level of GAS6 was associated with higher rates of all-cause and cardiovascular mortality (P<0.05). Baseline plasma GAS6 levels were still strongly correlated with clinical outcomes in different models after adjustment for clinical factors and N-terminal pro-brain natriuretic peptide (NT-proBNP, P<0.05). GAS6 could further distinguish the risks of clinical outcomes based on the NT-proBNP measurement. Conclusion: We demonstrated that elevated plasma GAS6 levels were associated with an increased risk of all-cause and cardiovascular death in patients with AHF. Registration Number: NCT03727828 (DRAGON-HF trial) https://clinicaltrials.gov/ct2/show/NCT03727828?term=NCT03727828&draw=2&rank=1.
Frictionless vs. frictional contact in numerical wear predictions of conformal and non-conformal sliding couplings
Authors: Mattei Lorenza , Puccio Francesca Di 
Published: August 2022

Abstract
The role of friction on wear evolution is manifold since it interplays with lubrication regime, nominal contact point and contact pressure distribution. Nevertheless, in the literature many wear models simulate wear assuming frictionless contact conditions to simplify the analyses. That assumption, physically not realistic, often appears as contradiction, permitted in numerical simulations where friction and wear can be considered independent phenomena. This study aims to validate the frictionless assumption in wear models with steady nominal contact point, such us common configurations, e.g. pin-on-plate/pin-on-disc. Wear was simulated according to the Archard wear law for both non-conformal and conformal pin-on-plate contact pairs in reciprocating motion, assuming frictionless and frictional contact conditions, varying the coefficient of friction f in the range 0-0.4. Finite Element wear models were developed in Ansys® both with implicit and explicit kinematics. Results demonstrate that the effect of friction on contact pressure distribution and worn profiles, and on their evolution is negligible (differences lower than 0.05%). Thus, wear can be predicted using models in frictionless conditions which allow to extremely reduce the computational costs, that represent a limit of FE wear simulations. Additionally, a procedure with implicit kinematics was compared to the explicit one resulting valid and computationally convenient, especially in case of non-conformal contact.
Prognostic value of postoperative radiotherapy in patients with vulvar squamous carcinoma: findings based on the SEER database
Authors: Li Miaomiao , Li Jing , Wang Zanhong 
Published: August 2022

Abstract
Introduction: The role of postoperative radiotherapy in treating squamous cell carcinoma of the vulva remains controversial. This study evaluated the effect of radiotherapy on the survival of patients with postoperative squamous cell carcinoma of the vulva.Methods： Clinical and prognostic information on patients diagnosed with vulvar squamous cell carcinoma from 2010 to 2015 was collected from the Surveillance, Epidemiology, and Prognosis (SEER) database. A propensity score matching (PSM) approach was used to balance the differences in clinicopathological characteristics between groups. The impact of postoperative radiotherapy on overall survival (OS) and disease-specific survival (DSS) was assessed.Results： The study included 3629 patients with squamous cell carcinoma of the vulva, of whom 767 (21.1%) underwent postoperative radiotherapy. After propensity score matching, multivariate analysis showed that Age, M stage, tumor size, and lymph node surgery were independent prognostic factors affecting patient survival. Postoperative radiotherapy improved patients' overall survival and disease-specific survival. Further subgroup survival analysis showed that overall survival was significantly improved among patients who received radiotherapy in patients with Grade III, IV, AJCC stage III, N1, lymph node metastasis, large tumor diameter, and those who received chemotherapy.Conclusion: Postoperative radiotherapy may provide a survival benefit for patients with squamous cell carcinoma of the vulva, especially for AJCC stage III, lymph node metastases, large tumor diameter, and those receiving postoperative chemotherapy.
SSD with Multi-scale feature Fusion and Attention mechanism
Authors: Zeng Zhigao , Dong Lijun , Liu Qiang , Zhu Wenqiu , Zhu Yanhui , Meng Chen 
Published: August 2022

Abstract
In the Internet of Things, image acquisition equipment is very important, which will generate lots of invalid data when monitoring in real time. Analyzing the data collected from the terminal directly by edge calculation can remove invalid frames and improve the accuracy of system detection. SSD algorithm model is relatively light and fast detection speed. However, SSD algorithms don't take full advantage of both shallow and deep information, a multiscale feature fusion attention mechanism structure based on SSD algorithm was proposed in this paper, which combines the idea of multiscale feature fusion and attention mechanism. Improve the feature information expression ability by fusing adjacent feature layers for each detection layer. Then, add the attention mechanism to to improve the algorithm's attention to the feature map channels. The results of the experiment show that the optimized model detection accuracy is improved, which will greatly improve the reliability of edge calculation.
Removal of hexavalent chromium from simulated wastewater by Polyethylene glycol modified D201 resin supported nanoscale zero-valent iron
Authors: Wang Shuang , Zhong Dengjie , Xu Yunlan , Zhong Nianbing 
Published: August 2022

Abstract
In view of the increasingly prominent heavy metal pollution problem in water, polyethylene glycol modified D201 resin supported nanoscale zero-valent iron (PEG-nZVI/D201) was fabricated to remove Cr(VI) in wastewater. PEG-nZVI/D201 can overcome the agglomeration and oxidation drawbacks of nZVI effectively, and improve the removal efficiency of Cr(VI). Its Cr(VI) removal rate was 33.6% higher than that of bare nZVI when the dosage of PEG-nZVI/D201 was 1.0 g·L− 1, the Cr(VI) concentration was 50 mg·L− 1 and initial pH was 5.0. The Langmuir model better describes the adsorption of Cr(VI) on PEG-nZVI/D201. Thermodynamic parameters show that adsorption is spontaneous and endothermic. The mechanism of Cr(VI) removal by PEG-nZVI/D201 is as follows: firstly, protonated PEG-nZVI/D201 adsorbs Cr(VI) by electrostatic attraction, then nZVI reduces most of Cr(VI) to Cr(III) through its strong reducibility, and finally Cr(III) can form CrXFe1−X(OH)3 co-precipitation with Fe(III) produced by oxidation of nZVI.
Activation of CREB-BDNF pathway in pyramidal neurons in hippocampus improves the neurological outcome of mice with ischemic stroke
Authors: Jiang Yingying , Liu Qingying , Zhao Yumei , Wang Chunyang , Sun Ming 
Published: August 2022

Abstract
Cerebral ischemia is characterized by several pathological reaction evolving over time. Hyperactivation of glutamatergic neurons is the main factor leading to excitotoxicity which potentiates oxidative stress and triggers the mechanisms of neural apoptosis after cerebral ischemia. However, it is unclear whether glutamate in the ventral hippocampal Cornus Ammonis 1 (vCA1) acts a part in neurological deficits, pain perception, anxiety and depression induced by ischemic-stroke. We investigated the effects of chemogenetic inhibition or activation of vCA1 pyramidal neurons which are mainly glutamatergic neurons on sequelae induced by cerebral ischemia. Our results revealed that inhibition of vCA1 pyramidal neurons by chemogenetics alleviated neurological deficits, pain perception, anxiety and depression caused by cerebral ischemia in mice, but activation of vCA1 pyramidal neurons had limited effects. Moreover, we found that stroke was accompanied by decreased levels of cAMP-response element-binding protein (CREB) and brain-derived neurotrophic factor (BDNF) in vCA1, which are modulated by glutamate. In this study, overexpression of CREB protein in pyramidal neurons in vCA1 by AAV virus significantly upregulated the content of BDNF and ameliorated the dysfunction induced by ischemic-stroke. Our results demonstrated activation of CREB-BDNF pathway in vCA1 pyramidal neurons significantly improved neurological deficits, pain perception, anxiety and depression induced by ischemic-stroke.
Predicting Land Use/Land Cover Changes in the Lesser Zab River Catchment/Iraq through CA-Markov Synergy Model
Authors: Mahdi Zahra Ali , Mohammed Ruqayah Kadhim 
Published: August 2022

Abstract
Land use/land cover is measured as one of the utmost dynamic constituents of the atmosphere that has been altering abnormally from the time when after the industrial revolution at different measures. A well understanding of the drive and strength of environments needs regular monitoring and quantifying for land use/land cover alteration changing aspects. The current research targets to predict the prospect land use/land cover (LU/LC) alterations, for the Lesser Zab catchment in the Northern part of Iraq, applying the synergy Cellular Automata-Markov simulation. Three sequential year Landsat images (1999, 2010, and 2021) were categorized by the Maximum Likelihood method. Then, three LU/LC images with numerous class classifications were created and an alteration identification examination was performed. With the categorized (1999–2010) as well as (2010–2021) LU/LC maps in the hybrid model, the corresponded LU/LC maps for 2021 and 2041 were modeled, correspondingly. The classified 2021 LU/LC map was considered to validate model output 2021. The agreement accuracy between the categorised and the modeled images were Kno = 0.864, Klocation = 0.854, Kstandard = 0.785, in that order. Prospect likelihoods validate that between 2021 and 2041, the urban area would rise by 78% (from 1118 to 5200 km2). However, bare lands/light, agricultural lands, water bodies, bare lands/dark, and forest lands would decrease by 3% (from 6983 to 6736 km2), 12% (from 7992 to 7036 km2), 15% (from 141.03 to 119.86 km2), 30% (from 7 to 4 km2), and 76% (from 3810 to 904 km2), correspondingly. This study’s conclusions are priceless for policymakers, urban managers, and ecological researchers.
Transcriptomic mapping of key reproductive and metabolic tissues and oocytes in mouse models of polycystic ovary syndrome
Authors: Deng Qiaolin , Pei Yu , Risal Sanjiv , Jiang Hong , Lu Jiang , Lindgren Eva , Stener-Victorin Elisabet 
Published: August 2022

Abstract
Excessive androgen production and obesity are key to Polycystic ovary syndrome (PCOS) pathogenesis. PCOS-like mouse models induced by androgen exposure include the prenatal androgenized (PNA), peripubertal androgenized, and overexpression of nerve growth factor in theca cells (17NF), as well as the effect of diet-induced maternal obesity model on offspring. To reveal the molecular features of these models, we performed transcriptomic profiling of the hypothalamus, adipose tissue, ovary, and MII oocytes. The largest number of differentially expressed genes (DEGs) were found in the ovaries of 17NF and in the adipose tissues of peripubertal androgenized models. In contrast, the hypothalamus is most affected in PNA and maternal obesity models suggesting fetal programming effects. The Ms4a6e gene, membrane-spanning 4-domains subfamily A member 6E, a DEG identified in the adipose tissue in all PCOS-like mouse models is also differently expressed in adipose tissue of women with PCOS women, highlighting a conservative disease mechanism. Our comprehensive transcriptomic mapping of key target tissues of the PCOS pathology provides a unique resource when investigating molecular mechanisms induced by androgen exposure and highlights that there are critical windows for androgen administration and maternal obesity.
A novel systemic inflammatory-nutritional score (INS) and nomogram for the prognostic prediction of adult head and neck soft tissue sarcoma in patients undergoing R0 surgery
Authors: Xu Bushu , Qiu Haibo , Ou Biyi , Chen Shuwei , Du Dingfu , Liu Yuanyuan , Zhang Xing , Liang Yao 
Published: August 2022

Abstract
Background
Head and neck soft tissue sarcoma (HNSTS) comprises a rare group of malignancies with high risks of recurrence and mortality. However, the biomarkers predicting the prognosis of HNSTS patients received R0 resection are limited. Here, we aimed to develop a novel inflammatory-nutritional score (INS) system and a nomogram to explore the prognostic value of preoperative biomarkers in HNSTS patients.
Methods
With a median follow-up time of 134.6 months, 315 patients with HNSTS who underwent R0 resection were enrolled. Clinical characteristics and hematological features were collected. The least absolute shrinkage and selection operator Cox regression model was used to select the most significant survival‑associated candidate for constructing the INS. The relationship between the INS groups and clinical features was analyzed. Further, a nomogram based on the INS and clinical features was generated by multivariate Cox analysis, assessed by the concordance index and internally validated by bootstraps.
Results
The five-year overall survival (OS) rate, three-year recurrence-free survival rate and disease-free survival (DFS) rate were 77.3%, 61.0% and 55.4%, respectively. After scoring, the novel INS system was established and divided into 3 groups: Low Risk (INS 0), Medium Risk (INS 1–3) and High Risk (INS 4–7). High INSs were associated with a deep tumor location (p < 0.001), high tumor grade (p < 0.001), and advanced American Joint Committee on Cancer stage (p < 0.001). The Low-Risk group exhibited a higher 5-year OS rate and 3-year DFS rate than the Medium-Risk and High-Risk groups (87.6% versus 81.3%% versus 53.3%, p < 0.001; 62.2% versus 56.9% versus 37.9%, p = 0.007). Moreover, the multivariate analysis demonstrated that the INS (p = 0.023), tumor depth (p < 0.001), pT stage (p = 0.022), pN stage (p < 0.001) and tumor grade (p < 0.001) were independent survival predictors for HNSTS patients. Based on these results, a novel prognostic nomogram for OS was generated, which showed a better performance than the p7TNMG stage alone (p < 0.001).
Conclusions
Our findings suggest that oncological outcomes among HNSTS patients who underwent R0 resection can be accurately predicted using the INS, therefore, an INS-based specific nomogram provides reliable, individualized prognostic information for patients with HNSTS to guide treatment strategies in clinical practice.