bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neutrophils predominate the immune signature of cerebral thrombi in COVID-19 stroke patients
Authors: Genchi Angela , Semerano Aurora , Schwarz Ghil , Dell’Acqua Beatrice , Gullotta Giorgia Serena , Sampaolo Michela , Boeri Enzo , Quattrini Angelo , Sanvito Francesca , Diamanti Susanna , Bergamaschi Andrea , Grassi Stefano , Podini Paola , Panni Pietro , Michelozzi Caterina , Simionato Franco , Scomazzoni Francesco , Remida Paolo , Valvassori Luca , Falini Andrea , Ferrarese Carlo , Michel Patrik , Saliou Guillaume , Hajdu Steven , Beretta Simone , Roveri Luisa , Filippi Massimo , Strambo Davide , Martino Gianvito , Bacigaluppi Marco 
Published: February 2022

AbstractCoronavirus disease 2019 (COVID-19) is associated with an increased risk of thrombotic events. Ischemic stroke in COVID-19 patients entails high severity and mortality rates. Here we aimed to analyze cerebral thrombi of COVID-19 patients with large vessel occlusion (LVO) acute ischemic stroke to expose molecular evidence for SARS-CoV-2 in the thrombus and to unravel any peculiar immune-thrombotic features. We conducted a systematic pathological analysis of cerebral thrombi retrieved by endovascular thrombectomy in patients with LVO stroke infected with COVID-19 (n = 7 patients) and non-covid LVO controls (n = 23). In thrombi of COVID-19 patients, the SARS-CoV-2 docking receptor ACE2 was mainly expressed in monocytes/macrophages and showed higher expression levels compared to controls. Using polymerase chain reaction and sequencing, we detected SARS-CoV-2 Clade20A, in the thrombus of one COVID-19 patient. Comparing thrombus composition of COVID-19 and control patients, we noted no overt differences in terms of red blood cells, fibrin, neutrophil extracellular traps (NETs), von Willebrand Factor (vWF), platelets and complement complex C5b-9. However, thrombi of COVID-19 patients showed increased neutrophil density (MPO+ cells) and a three-fold higher Neutrophil-to-Lymphocyte Ratio (tNLR). In the ROC analysis both neutrophils and tNLR had a good discriminative ability to differentiate thrombi of COVID-19 patients from controls. In summary, cerebral thrombi of COVID-19 patients can harbor SARS-CoV2 and are characterized by an increased neutrophil number and tNLR and higher ACE2 expression. These findings suggest neutrophils as the possible culprit in COVID-19-related thrombosis.
Graphical Abstract
A review of the pathogenicity mechanism of Verticillium dahliae in cotton
Authors: ZHANG Yalin , ZHOU Jinglong , ZHAO Lihong , FENG Zili , WEI Feng , BAI Hongyan , FENG Hongjie , ZHU Heqin 
Published: February 2022

AbstractVerticillium wilt, caused by the notorious fungal pathogen Verticillium dahliae, is one of the main limiting factors for cotton production. Due to the stable dormant structure microsclerotia, long-term variability and co-evolution with host plant, its pathogenicity mechanism is very complicated, and the interaction mechanism between pathogen and host plant is also unclear. So identification and functional analysis of the genes involved in the pathogenicity or virulence of this fungus will benefit to uncover the molecular pathogenic mechanism of V. dahliae. In this review, many multifunction genes covering microsclerotia development, pathogen infection, effector proteins, transcription factors, horizontal gene transfer and trans-kingdom RNA silencing have been summarized to provide a theoretical basis to deep understand the molecular pathogenicity mechanism of V. dahliae and promote to effectively control Verticillium wilt. Furtherly, these pathogenicity-related genes may be considered as targets for effective control of Verticillium wilt in cotton.
Iterative analytic extension in tomographic imaging
Authors: Zeng Gengsheng L. 
Published: February 2022

AbstractIf a spatial-domain function has a finite support, its Fourier transform is an entire function. The Taylor series expansion of an entire function converges at every finite point in the complex plane. The analytic continuation theory suggests that a finite-sized object can be uniquely determined by its frequency components in a very small neighborhood. Trying to obtain such an exact Taylor expansion is difficult. This paper proposes an iterative algorithm to extend the measured frequency components to unmeasured regions. Computer simulations show that the proposed algorithm converges very slowly, indicating that the problem is too ill-posed to be practically solvable using available methods.
Narrative review of the COVID-19, healthcare and healthcarers thematic series
Authors: Williams Richard , Kaufman Kenneth R. 
Published: February 2022

SummaryThis review covers the thematic series of 22 papers selected from among manuscripts published by BJPsych Open concerning coronavirus disease 2019 (COVID-19) and healthcare. We report nine papers that cover concepts and epidemiology relating to the public and patients. We review 11 papers about the impact of COVID-19 on healthcare services and their staff in 15 countries. Two papers consider the psychosocial impact on staff working in mental health services in the UK. Most papers report cross-sectional analyses of data collected from convenience samples by self-reported surveys conducted at single times. They have limitations of generalisability, do not enable conclusions about diagnosis or causality, and many are likely to have attendant bias and noise. BJPsych Open published these papers to meet requirements for early indications of the mental health impact of COVID-19 on the public and on healthcare staff. They claim high prevalence of symptoms of anxiety, depression and post-traumatic stress. We contrast these findings with selected reports of studies with different methodologies published elsewhere. We emphasise the need for longitudinal clinical studies with refined sampling and methodological rigour. We identify several longitudinal research programmes; two in this series. We advocate tuning advice offered about caring for the public and healthcare staff to the realities of their circumstances and their perceptions of need in the context of findings from further longitudinal studies. We draw attention to the importance of the social, relationship and environmental circumstances of the public and healthcare staff in order to understand their distress and their risks of developing mental health disorders.
Policy Brief 2: Social norms, mental models and other behavioural drivers of petty corruption
Authors: Baez Camargo Claudia , Stahl Cosimo 
Published: February 2022

This policy brief summarises the main findings and lessons learned from research on corruption, social norms and behaviours in Uganda. The empirical evidence indicates that behavioural factors associated to social practices and collective understandings play a role in shaping Ugandan citizens’ attitudes towards petty corruption and in fuelling practices such as bribery and favouritism.
On the basis of the research findings, policy recommendations are put forward aiming to contribute to the development of anti-corruption interventions that incorporate behavioural insights in their design and implementation.
Unprecedented early-summer heat stress and forecast of coral bleaching on the Great Barrier Reef, 2021-2022
Authors: Spady Blake L. , Skirving William J. , Liu Gang , De La Cour Jacqueline L. , McDonald Cathy J. , Manzello Derek P. 
Published: February 2022

The Great Barrier Reef (GBR) is predicted to undergo its sixth mass coral bleaching event during the Southern Hemisphere summer of 2021-2022. Coral bleaching-level heat stress over the GBR is forecast to start earlier than any previous year in the satellite record (1985-present). The National Oceanic and Atmospheric Administration (NOAA) Coral Reef Watch (CRW) near real-time satellite-based heat stress products were used to investigate early-summer sea surface temperature (SST) and heat stress conditions on the GBR during late 2021. As of 14 December 2021, values of instantaneous heat stress (Coral Bleaching HotSpots) and accumulated heat stress over a 12-week running window (Degree Heating Weeks) on the GBR were unprecedented in the satellite record. Further, 89% of GBR satellite reef pixels for this date in 2021 had a positive seven-day SST trend of greater than 0.2 degrees Celsius/week. Background temperatures (the minimum temperature over the previous 29 days) were alarmingly high, with 87% of GBR reef pixels on 14 December 2021 being greater than the maximum SST over that same 29-day period for any year from 1985-2020. The GBR is starting the 2021-2022 summer season with more accumulated heat than ever before, which could have disastrous consequences for the health, recovery, and future of this critical reef system.
Effects of asparaginases and L-carnitine on Western-diet-induced hepatosteatosis in mice
Authors: Ali Mahmoud Assar Mona , Hüffel Martina , Afify Mamdouh , Weiskirchen Ralf , Eisert Albrecht , Tolba Rene , Steitz Julia 
Published: February 2022

Abstract Background: Asparaginases are common chemotherapeutic agents used for the treatment of acute lymphoblastic leukemia as a single or combinational therapy. Accompanying hepatotoxicity makes its use in elderly patients with pre-conditions, as obesity or other hepatopathies, difficult. Various hepatoprotective compounds like, L-carnitine, are discussed to ameliorate the induced hepatotoxicity. Methods: Here we aimed to establish a mouse model to study the effect of asparaginases (L-asparaginase and Oncaspar) and L-carnitine on Western-diet-induced hepatosteatosis in mice. Dose-escalation studies were performed to analyze asparaginases induced hepatotoxicity in C57BL/6 mice with normal or fatty livers. Subsequently, the effect of L-carnitine to improve the induced toxicity was tested. Results: Our results showed mild-to-moderate hepatotoxic effects while the Western-diet induced a higher degree of vacuolization and hepatocyte damage in liver tissue. Testing of L-carnitine in the established models did not show any protective effect on the toxicity or impairment of the efficacy of asparaginases. Conclusion: The here established models were able to demonstrate the asparaginase-induced hepatotoxic effects which were enhanced by the Western-diet. However, to test potential ameliorating drugs, the models might need some improvements.
The Effects of Online Review Platforms on Restaurant Revenue, Consumer Learning, and Welfare
Authors: Fang Limin 
Published: February 2022

This paper quantifies the effects of online review platforms on restaurant revenue and consumer welfare. Using a novel data set containing revenues and information from major online review platforms in Texas, I show that online review platforms help consumers learn about restaurant quality more quickly. The effects on learning show up in restaurant revenues. Specifically, doubling the review activity increases the revenue of a high-quality independent restaurant by 5%–19% and decreases that of a low-quality restaurant by a similar amount. These effects vary widely across restaurants’ locations. Restaurants around highway exits are affected twice as much as those in nonhighway areas, implying that reviews are more useful to travelers and tourists than locals. The effects also decline as restaurants age, consistent with the diminishing value of information in learning. In contrast, chain restaurants are affected to a much lesser degree than independent restaurants. Building on this evidence, I develop a structural demand model with aggregate social learning. Counterfactual analyses indicate that online review platforms raise consumer welfare much more for tourists than for locals. By encouraging consumers to eat out more often at high-quality independent restaurants, online review platforms increased the total industry revenue by 3.0% over the period from 2011–2015. This paper was accepted by Matthew Shum, marketing. Supplemental Material: Data and the online appendix are available at https://doi.org/10.1287/mnsc.2021.4279 .
CircSCAPER contributes to IL-1β-induced osteoarthritis in vitro via miR-140-3p/EZH2 axis
Authors: Luobu Zhaxi , Wang Lei , Jiang Dahai , Liao Tao , Luobu Ciren , Qunpei Luosong 
Published: February 2022

Aims Circular RNA (circRNA) S-phase cyclin A-associated protein in the endoplasmic reticulum (ER) (circSCAPER, ID: hsa_circ_0104595) has been found to be highly expressed in osteoarthritis (OA) patients and has been associated with the severity of OA. Hence, the role and mechanisms underlying circSCAPER in OA were investigated in this study. Methods In vitro cultured human normal chondrocyte C28/I2 was exposed to interleukin (IL)-1β to mimic the microenvironment of OA. The expression of circSCAPER, microRNA (miR)-140-3p, and enhancer of zeste homolog 2 (EZH2) was detected using quantitative real-time polymerase chain reaction and Western blot assays. The extracellular matrix (ECM) degradation, proliferation, and apoptosis of chondrocytes were determined using Western blot, cell counting kit-8, and flow cytometry assays. Targeted relationships were predicted by bioinformatic analysis and verified using dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. The levels of phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway-related protein were detected using Western blot assays. Results CircSCAPER was highly expressed in OA cartilage tissues and IL-1β-induced chondrocytes. Knockdown of circSCAPER reduced IL-1β-evoked ECM degradation, proliferation arrest, and apoptosis enhancement in chondrocytes. Mechanistically, circSCAPER directly bound to miR-140-3p, and miR-140-3p inhibition reversed the effects of circSCAPER knockdown on IL-1β-induced chondrocytes. miR-140-3p was verified to target EZH2, and overexpression of miR-140-3p protected chondrocytes against IL-1β-induced dysfunction via targeting EZH2. Additionally, we confirmed that circSCAPER could regulate EZH2 through sponging miR-140-3p, and the circSCAPER/miR-140-3p/EZH2 axis could activate the PI3K/AKT pathway. Conclusion CircSCAPER promoted IL-1β-evoked ECM degradation, proliferation arrest, and apoptosis enhancement in chondrocytes via regulating miR-140-3p/EZH2 axis, which gained a new insight into the pathogenesis of OA. Cite this article: Bone Joint Res 2022;11(2):61–72.
Fracture-related infection in osteoporotic bone causes more severe infection and further delays healing
Authors: Li Jie , Wong Ronald Man Yeung , Chung Yik Lok , Leung Sharon Shui Yee , Chow Simon Kwoon-Ho , Ip Margaret , Cheung Wing-Hoi 
Published: February 2022

Aims With the ageing population, fragility fractures have become one of the most common conditions. The objective of this study was to investigate whether microbiological outcomes and fracture-healing in osteoporotic bone is worse than normal bone with fracture-related infection (FRI). Methods A total of 120 six-month-old Sprague-Dawley (SD) rats were randomized to six groups: Sham, sham + infection (Sham-Inf), sham with infection + antibiotics (Sham-Inf-A), ovariectomized (OVX), OVX + infection (OVX-Inf), and OVX + infection + antibiotics (OVX-Inf-A). Open femoral diaphysis fractures with Kirschner wire fixation were performed. Staphylococcus aureus at 4 × 104 colony-forming units (CFU)/ml was inoculated. Rats were euthanized at four and eight weeks post-surgery. Radiography, micro-CT, haematoxylin-eosin, mechanical testing, immunohistochemistry (IHC), gram staining, agar plating, crystal violet staining, and scanning electron microscopy were performed. Results Agar plating analysis revealed a higher bacterial load in bone (p = 0.002), and gram staining showed higher cortical bone colonization (p = 0.039) in OVX-Inf compared to Sham-Inf. OVX-Inf showed significantly increased callus area (p = 0.013), but decreased high-density bone volume (p = 0.023) compared to Sham-Inf. IHC staining showed a significantly increased expression of TNF-α in OVX-Inf compared to OVX (p = 0.049). Significantly reduced bacterial load on bone (p = 0.001), enhanced ultimate load (p = 0.001), and energy to failure were observed in Sham-Inf-A compared to Sham-Inf (p = 0.028), but not in OVX-Inf-A compared to OVX-Inf. Conclusion In osteoporotic bone with FRI, infection was more severe with more bone lysis and higher bacterial load, and fracture-healing was further delayed. Systemic antibiotics significantly reduced bacterial load and enhanced callus quality and strength in normal bone with FRI, but not in osteoporotic bone. Cite this article: Bone Joint Res 2022;11(2):49–60.
Crab diversity and crab potential as support ecotourism in Teleng Ria, Grindulu and Siwil Beach, Pacitan, East Java, Indonesia
Authors: IRWANSYAH ROMZI MAULANA , AZZAHRA SAVITRI INTAN NUR , DARMASTUTI SALMA ARDELIA , RAMADHANDI ANANDA RILO , FIRDAUS OLIVIA , DAENI FITRI , SAFITRI NADIRA , FAJRI OCTA PRAMESWARI AMBANG , NUGROHO GILANG DWI , NAIM DARLINA MD. , SETYAWAN AHMAD DWI 
Published: February 2022

Abstract. Irwansyah RM, Azzahra SIN, Darmastuti SA, Ramadhandi AR, Firdaus O, Daeni F, Safitri N, Fajri OPA, Nugroho GN, Naim DM, Setyawan AD. 2021. Crab diversity and crab potential as support ecotourism in Teleng Ria, Grindulu and Siwil Beach, Pacitan, East Java, Indonesia. Intl J Bonorowo Wetlands 11: 75-83. The mangrove area in Pacitan District, Pacitan, East Java, Indonesia is generally not polluted. Therefore, the land is suitable for growing conditions from mangrove plants and has great potential as a mangrove area with ecotourism management in Indonesia. Ecotourism activities in mangrove areas, in principle, are the use of mangrove areas while maintaining the biological/ecological functions of mangrove areas that have social and economic value for local community. The mangrove ecosystem is a habitat of various species of Crustacea, such as the crab. Crabs are live in coastal/mangrove ecosystems and one of the key species that have a very important role in maintaining the balance of the ecosystem. The study about diversity of crabs in the mangrove area is very important because it will improve the quality of mangrove and potentially support ecotourism in the mangrove ecosystem. This study aims to determine the diversity of the crab and its potential to support ecotourism if the Pacitan Beaches will be ecotourism in the future. This research was conducted in Teleng Ria Beach, Grindulu Beach, and Siwil Beach, Pacitan, East Java, Indonesia in November 2021. Plots of 10 x 10 m2 are made to record the species and the number of individual crab species. The result found five species of crab,i.e. Austruca annulipes (H. Milne-Edwards, 1837), Coenobita perlatus (H. Milne-Edwards, 1837), Ocypode kuhlii (De Haan, 1835), Perisesarma guttatum (A. Milne-Edwards, 1869), and Scylla serrata (Forsskål, 1775). The total crab diversity index of 1.25 is included in the medium category. The morphology, activity, number of individuals and distribution of each crabs species in an ecotourism area will increase the attractiveness of tourists to visit. For example, the morphology of C. perlatus that has the red color as a strawberry sometimes has a home/the shells of the Mollusca which color and unique shape so that add appeal to be seen. Then, the crab A. annulipes that like to dance and play the violin with the claw can also be attractions drawing tourists. Hopefully, the data can be a reference for the managers of the mangrove area in developing ecotourism and conservation of mangrove forest.
High atherogenic risk concomitant with elevated HbA1c among persons with type 2 diabetes mellitus in North Ethiopia
Authors: Gebreyesus Hagos Amare , Abreha Girmatsion Fisseha , Besherae Sintayehu Degu , Abera Merhawit Atsbha , Weldegerima Abraha Hailu , Gidey Aregawi Haileslassie , Bezabih Afework Mulugeta , Lemma Tefera Belachew , Nigatu Tsinuel Girma 
Published: February 2022

Background
Type 2 diabetes mellitus (T2DM) is a chronic disease associated with worse clinical presentation. However, the current investigation practices in Ethiopia have limitations to demonstrate the scope of the clinical burden. Hence, this study was aimed at assessing the glycemic status and coronary heart disease (CHD) risk of persons with T2DM using HbA1c and atherogenic index of plasma (AIP).

Method
This institution-based cross-sectional study was conducted among 421 adults with T2DM from September to November 2019. Demographic, socioeconomic, and lifestyle data were collected through a face-to-face interview. Clinical information was retrieved from medical records whereas anthropometric and biochemical measurements were performed using the WHO protocols. Glycemic status was determined using HbA1c and CHD risk assessed using an atherogenic index of plasma (AIP). Gaussian variables were expressed using mean and standard deviation (SD), Log-normal variables using geometric mean and 95% CI and non- Gaussian variables using median and interquartile ranges. Categorical variables were summarized using absolute frequencies and percentages. Multivariable logistic regression was used to identify factors associated with glycemic control with a statistical significance set at 5%.

Result
A total of 195 male and 226 female subjects were involved in this study. The results demonstrated that 77% (324) had HbA1c value ≥7% and 87.2% (367) had high atherogenic risk for CHD. Besides, 57% and 67.9% of persons with T2DM had metabolic syndrome according to International Diabetes Federation (IDF) and the National Cholesterol Education Program—Adult treatment panel III (NCEP-ATP III) criteria, respectively. About 36.8% had one or more comorbidities. Having healthy eating behavior [AOR 1.95; CI 1.11–3.43] and taking metformin [AOR 4.88; CI 1.91–12.44] were associated with better glycemic outcomes.

Conclusion
High AIP level concomitant with poor glycemic control indicates increased risk for coronary heart disease among persons with T2DM in Northern Ethiopia.
miR-25-3p protects renal tubular epithelial cells from apoptosis induced by renal IRI by targeting DKK3
Authors: Zhang Yu , Zuo Xiangrong 
Published: February 2022

Abstract
Renal ischemia-reperfusion injury (IRI) is one of the main causes of acute kidney injury (AKI). So far, there have been many studies on renal IRI, although an effective treatment method has not been developed. In recent years, growing evidence has shown that small noncoding RNAs play an important regulatory role in renal IRI. This article aims to explore whether microRNA-25-3p (miR-25-3p) plays a role in the molecular mechanism of renal IRI. The results showed that the expression level of miR-25-3p was significantly downregulated in a rat renal IRI model, and this result was confirmed with in vitro experiments. After the hypoxia-reoxygenation treatment, the apoptosis level of NRK-52E cells transfected with miR-25-3p mimics decreased significantly, and this antiapoptotic effect was antagonized by miR-25-3p inhibitors. In addition, we confirmed that DKK3 is a target of miR-25-3p. miR-25-3p exerts its protective effect against apoptosis on NRK-52E cells by inhibiting the expression of DKK3, and downregulating the expression level of miR-25-3p could disrupt this protective effect. In addition, we reconfirmed the role of miR-25-3p in rats. Therefore, we confirmed that miR-25-3p may target DKK3 to reduce renal cell damage caused by hypoxia and that miR-25-3p may be a new potential treatment for renal IRI.