bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 Infection of Microglia Elicits Pro-inflammatory Activation and Apoptotic Cell Death
Authors: Jeong Gi Uk , Lyu Jaemyun , Kim Kyun-Do , Chung Young Cheul , Yoon Gun Young , Lee Sumin , Hwang Insu , Shin Won-Ho , Ko Junsu , Lee June-Yong , Kwon Young-Chan 
Published: January 2022

ABSTRACTAccumulating evidence suggests that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes various neurological symptoms in coronavirus disease 2019 (COVID-19) patients. The most dominant immune cells in the brain are microglia. Yet, the relationship between neurological manifestations, neuroinflammation, and host immune response of microglia to SARS-CoV-2 has not been well characterized. Here, we report that SARS-CoV-2 can directly infect human microglia, eliciting M1-like pro-inflammatory responses, followed by cytopathic effects. Specifically, SARS-CoV-2 infected human microglial clone 3 (HMC3), leading to inflammatory activation and cell death. RNA-seq analysis also revealed that ER stress and immune responses were induced in the early and apoptotic processes in the late phase of viral infection. SARS-CoV-2-infected HMC3 showed the M1 phenotype and produced pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumour necrosis factor α (TNF-α), but not the anti-inflammatory cytokine IL-10. After this pro-inflammatory activation, SARS-CoV-2 infection promoted both intrinsic and extrinsic death receptor-mediated apoptosis in HMC3. Using K18-hACE2 transgenic mice, murine microglia were also infected by intranasal inoculation of SARS-CoV-2. This infection induced the acute production of pro-inflammatory microglial IL-6 and TNF-α and provoked a chronic loss of microglia. Our findings suggest that microglia are potential mediators of SARS-CoV-2-induced neurological problems and, consequently, can be targets of therapeutic strategies against neurological diseases in COVID-19 patients.IMPORTANCERecent studies reported neurological manifestations and complications in COVID-19 patients, which are associated with neuroinflammation. As microglia are the dominant immune cells in brains, it needs to be elucidate the relationship between neuroinflammation and host immune response of microglia to SARS-CoV-2. Here, we suggest that SARS-CoV-2 can directly infect human microglia with cytopathic effect (CPE) using human microglial clone 3 (HMC3). The infected microglia were promoted to pro-inflammatory activation following apoptotic cell death. This pro-inflammatory activation was accompanied by the high production of pro-inflammatory cytokines, and led to neurotoxic-M1 phenotype polarization.In vivo, murine microglia were infected and produced pro-inflammatory cytokines and provoked a chronic loss using K18-hACE2 mice. Thus, our data present that SARS-CoV-2-infected microglia are potential mediators of neurological problems in COVID-19 patients. In addition, HMC3 cells are susceptible to SARS-CoV-2 and exhibit the CPE, which can be further used to investigate cellular and molecular mechanisms of neuroinflammation reported in COVID-19 patients.
Genomic Perspectives on the Emerging SARS-CoV-2 Omicron Variant
Authors: Ma Wentai , Yang Jing , Fu Haoyi , Su Chao , Yu Caixia , Wang Qihui , de Vasconcelos Ana Tereza Ribeiro , Bazykin Georgii A. , Bao Yiming , Li Mingkun 
Published: January 2022

AbstractA new variant of concern for SARS-CoV-2, Omicron (B.1.1.529), was designated by the World Health Organization on November 26, 2021. This study analyzed the viral genome sequencing data of 108 samples collected from patients infected with Omicron. First, we found that the enrichment efficiency of viral nucleic acids was reduced due to mutations in the region where the primers anneal to. Second, the Omicron variant possesses an excessive number of mutations compared to other variants circulating at the same time (62 vs. 45), especially in the Spike gene. Mutations in the Spike gene confer alterations in 32 amino acid residues, which was more than those observed in other SARS-CoV-2 variants. Moreover, a large number of nonsynonymous mutations occur in the codons for the amino acid residues located on the surface of the Spike protein, which could potentially affect the replication, infectivity, and antigenicity of SARS-CoV-2. Third, there are 53 mutations between the Omicron variant and its closest sequences available in public databases. Many of those mutations were rarely observed in the public database and had a low mutation rate. In addition, the linkage disequilibrium between these mutations were low, with a limited number of mutations (6) concurrently observed in the same genome, suggesting that the Omicron variant would be in a different evolutionary branch from the currently prevalent variants. To improve our ability to detect and track the source of new variants rapidly, it is imperative to further strengthen genomic surveillance and data sharing globally in a timely manner.
An elite broadly neutralizing antibody protects SARS-CoV-2 Omicron variant challenge
Authors: Zhou Biao , Zhou Runhong , Chan Jasper Fuk-Woo , Luo Mengxiao , Peng Qiaoli , Yuan Shuofeng , Mok Bobo Wing-Yee , Chen Bohao , Wang Pui , Poon Vincent Kwok-Man , Chu Hin , Chan Chris Chung-Sing , Tsang Jessica Oi-Ling , Chan Chris Chun-Yiu , Au Ka-Kit , Man Hiu-On , Lu Lu , To Kelvin Kai-Wang , Chen Honglin , Yuen Kwok-Yung , Chen Zhiwei 
Published: January 2022

ABSTRACTThe strikingly high transmissibility and antibody evasion of SARS-CoV-2 Omicron variant have posted great challenges on the efficacy of current vaccines and antibody immunotherapy.Here, we screened 34 BNT162b2-vaccinees and cloned a public broadly neutralizing antibody (bNAb) ZCB11 from an elite vaccinee. ZCB11 neutralized all authentic SARS-CoV-2 variants of concern (VOCs), including Omicron and OmicronR346K with potent IC50 concentrations of 36.8 and 11.7 ng/mL, respectively. Functional analysis demonstrated that ZCB11 targeted viral receptor-binding domain (RBD) and competed strongly with ZB8, a known RBD-specific class II NAb. Pseudovirus-based mapping of 57 naturally occurred single mutations or deletions revealed that only S371L resulted in 11-fold neutralization resistance, but this phenotype was not observed in the Omicron variant. Furthermore,prophylactic ZCB11 administration protected lung infection against both the circulating pandemic Delta and Omicron variants in golden Syrian hamsters. These results demonstrated that vaccine-induced ZCB11 is a promising bNAb for immunotherapy against pandemic SARS-CoV-2 VOCs.
Impact of OTT media on the society: insights from path analysis
Authors: Prasad S. Shyam 
Published: January 2022

PurposeThe proliferation of the internet has enabled over-the-top (OTT) media to spread rapidly. It is now at the threshold of creating a huge social impact. However, there have been no studies so far that have examined the impact of OTT media on society, family and individuals. This study proposes to investigate the same and discover its impact on the family, society and whether it has any effect on individuals' career or education and one's health.Design/methodology/approachPath analysis was used to investigate the patterns of the effect of OTT on the family and society. This method was chosen since it allows to examine the influences within a system of variables and gain insights into the OTT media's impact. The responses were collected through an online questionnaire.FindingsThe paper provides empirical insights into the impact of OTT media. It reveals some interesting and crucial insights on its influence.Research limitations/implicationsBecause of the chosen research approach, the research results do not establish any causal relationships. Since the survey was conducted during the pandemic, further research during “normal” time is suggested.Practical implicationsThe paper discloses the powerful and growing OTT media's significance.Social implicationsWith watching OTT emerging very rapidly as a powerful channel of entertainment, what people see is very important. Hence, the purpose of this empirical study to investigate the influence of OTT media on individual, family and society is crucial and topical.Originality/valueThis paper fulfils an identified need to understand OTT media's influence.
Natural resources and economic growth: evidence from Brunei Darussalam
Authors: Tahir Muhammad , Burki Umar , Hayat Arshad 
Published: January 2022

PurposeThis paper explores the relationship between natural resources and economic growth of Brunei Darussalam, an underresearched area in the available literature.Design/methodology/approachAnnual data are sourced from reliable sources for the period 1989–2020. Appropriate cointegration techniques for time series data are employed to estimate the specified models and extract results.FindingsThe results provide evidence about the positive and significant role that natural resources have played in the economic growth of Brunei Darussalam. Similarly, trade openness and domestic investment have also positively and significantly impacted the long-run economic growth. On the other hand, the impacts of government expenditure and the growth of human capital on economic growth are although positive but insignificant statistically in the long run. The short-run results show that natural resources, government expenditures and domestic investment have influenced economic growth both positively and significantly. Moreover, the positive and significant impact of trade openness on economic growth, which was observed in the long run, turned negative and insignificant in the short run. Finally, the insignificant positive relationship between the growth of human capital and economic growth observed in the long run remained the same in the short run.Originality/valueThis paper studies the resource curse hypothesis for Brunei Darussalam for the first time, and therefore, the findings will be of significant interest for policymakers and researchers.
“Just because people are old, just because they're ill…” dignity matters in district nursing
Authors: Stevens Emma , Price Liz , Walker Liz 
Published: January 2022

Purpose
This paper aims to explore the concept and practice, of dignity as understood and experienced by older adults and district nursing staff. The paper adds a new, nuanced, understanding of safeguarding possibilities in the context of district nursing care delivered in the home.

Design/methodology/approach
The research used an ethnographic methodology involving observations of care between community district nursing clinicians and patients (n = 62) and semi-structured interviews with nursing staff (n = 11) and older adult recipients of district nursing care (n = 11) in England.

Findings
Abuse is less likely to occur when clinicians are maintaining the dignity of their patients. The themes of time and space are used to demonstrate some fundamental ways in which dignity manifests. The absence of dignity offers opportunities for abuse and neglect to thrive; therefore, both time and space are essential safeguarding considerations. Dignity is influenced by time and how it is experienced temporally, but nurses are not allocated time to “do dignity”, an arguably essential component of the caregiving role, yet one that can become marginalised. The home-clinic exists as a clinical space requiring careful management to ensure it is also an environment of dignity that can safeguard older adults.

Practical implications
District nurses have both a proactive and reactive role in ensuring their patients remain safeguarded. By ensuring care is delivered with dignity and taking appropriate action if they suspect abuse or neglect, district nurses can safeguard their patients.

Originality/value
This paper begins to address an omission in existing empirical research regarding the role of district nursing teams in delivering dignified care and how this can safeguard older adults.
The relationship between healthcare service provision models and patient experience
Authors: De Rosis Sabina , Barchielli Chiara , Vainieri Milena , Bellé Nicola 
Published: January 2022

PurposeUser experience is key for measuring and improving the quality of services, especially in high personal and relation-intensive sectors, such as healthcare. However, evidence on whether and how the organizational model of healthcare service delivery can affect the patient experience is at an early stage. This study investigates the relationship between healthcare service provision models and patient experience by focusing on the nursing care delivery.Design/methodology/approach65 nurses' coordinators were involved to map the nursing models adopted in the healthcare organizations of in an Italian region, Tuscany. This dataset was merged with patient experience measures reported by 9,393 individuals discharged by the same organizations and collected through a Patient-Reported Experience Measures Observatory. The authors run a series of logistic regression models to test the relationships among variables.FindingsPatients appreciate those characteristics of care delivery related to a specific professional nurse. Having someone who is in charge of the patient, both the reference nurse and the supervisor, makes a real difference. Purely organizational features, for instance those referring to the team working, do not significantly predict an excellent experience with healthcare services.Research limitations/implicationsDifferent features referring to different nursing models make the difference in producing an excellent user experience with the service.Practical implicationsThese findings can support managers and practitioners in taking decisions on the service delivery models to adopt. Instead of applying monolithic pure models, mixing features of different models into a hybrid one seems more effective in meeting users' expectations.Originality/valueThis is one of the first studies on the relationship between provision models of high-contact and relational-intensive services (the healthcare services) and users' experience. This research contributes to the literature on healthcare service management suggesting to acknowledge the importance of hybridization of features from different, purely theoretical service delivery models, in order to fit with providers' practice and users' expectations.HighlightsThis is one of the first studies on the relationship between provision models of nursing care and patient experience.Healthcare services' users appreciate service delivery characteristics identified with “be cared by,” or in other words with having a reference nurse.Nursing models' features that relate to the organizations and that providers tend to judge as professionalizing and evolutive, such as team working, appear not key in relation to patient experience.Pure models of service delivery are theoretically useful, but hybrid models can better meet users' expectations.
Process facilitators shifting between the support and expert roles in a complex work environment intervention in the Swedish healthcare sector
Authors: Wikström Ewa , Severin Jonathan , Jonsdottir Ingibjorg H. , Akerstrom Magnus 
Published: January 2022

PurposeProcess facilitation as part of a complex intervention for changing or improving practices within workplaces is becoming a common work method. The aim of this study was to investigate what characterizes the process-facilitating role in a complex intervention.Design/methodology/approachThe present study focuses on a complex work environment intervention targeting eight organizational units (workplaces) in the Swedish healthcare sector. The study applies a mixed-method approach and has been carried out in two steps. First, a qualitative process evaluation was performed. Secondly, an evaluation was conducted to see to what extent these identified conditions and mechanisms affected the quantitative intervention effect in term of sickness absence.FindingsThe analysis shows that the facilitating role consisted of three overlapping and partially iterative phases. These phases involved different activities for the facilitating role. Depending on how the facilitating role and the intervention were designed, various supporting conditions were found to significantly affect the outcome of the intervention measured as the total sickness absence.Research limitations/implicationsIt is concluded that the facilitation is not static or fixed during the change process. Instead, the facilitation role develops and emerges through the process of support during the different implementation phases.Practical implicationsThe facilitative role of performing support is based on a combination of support role activities and expert role activities. The support role focuses on support activities, while the expert role includes capacity building through knowledge- and legitimacy-oriented activities.Originality/valueThis study contributes to earlier research by developing a methodological approach for carrying out process facilitation in complex interventions.
Analysis of determinants of youth self-employment career choices
Authors: Melak Degsew , Derbe Tegegne 
Published: January 2022

PurposeGiven the different manifestations of the unemployment crisis, the main purpose of this study was to identify what characteristics influence the participation of youth in key self-employment business options.Design/methodology/approachThe study has used both probability and nonprobability sampling techniques. Purposive sampling methods were used to identify target study areas (districts and Kebeles) while the systematic random sampling method was used to locate sample respondents. A total of 424 sample respondents were interviewed through interview scheduled questionnaires. Statistical data analysis was carried out using STATA 14 software.FindingsAgriculture, local value-added business activities, food-related services, petty trade and local transportation were common business choices where unemployed youths were engaged in. The findings of the study also showed that sex, loan size, loan repayment period and training gap were predictors of youth engagement in various self-employment career choices.Practical implicationsIncreasing loan size has a positive and significant influence on youth engagement in all self-employment business choices and has reminded us the need to revise or lift up loan size celling to assist youths in engaging in productive sectors. Similarly, the favourable correlation between female youths and value-added activities necessitates a well-designed female-specific intervention.Originality/valueAn understanding of the key determinants of youth preference to engage in specific self-employment career choices enables practitioners to intervene where necessary in supporting youth self-employment engagement. A combination of skill training, relaxed loan size and relaxed repayment is likely to gain sustainable business, which would benefit the local economy by transforming small businesses to a higher level and creating more job opportunities.
Fire performance of hybrid mass timber beam-end connections with perpendicular-to-wood grain reinforcement
Authors: Okunrounmu Oluwamuyiwa , Salem Osama (Sam) , Hadjisophocleous George 
Published: January 2022

PurposeThe fire resistance of timber structures is heavily dependent on the fire behaviour of the connections between its structural elements. The experimental study presented in this paper aimed to investigate the fire performance of glued-laminated timber beam connections reinforced perpendicular-to-wood grain with self-tapping screws (STS).Design/methodology/approachTwo full-size fire experiments were conducted on glulam beam-end connections loaded in flexure bending. Two connection configurations, each utilizing four steel bolts arranged in two different patterns, were reinforced perpendicular to wood grain using STS. The bolt heads and nuts and the steel plate top and bottom edges were fire protected using wood plugs and strips, respectively. Each connection configuration was loaded to 100% of the ultimate design load of the weakest unreinforced configuration. The test assemblies were exposed to elevated temperatures that followed the CAN/ULC-S101 standard fire time–temperature curve.FindingsThe experimental results show that the influence of the STS was significant as it prevented the occurrence of wood splitting and row shear-out and as a result, increased the fire resistance time of the connections. The time to failure of both connection configurations exceeded the minimum fire resistance rating specified as 45 min for combustible construction in applicable building codes.Originality/valueThe experimental data show the effectiveness of a simple fire protection system (i.e. wood plugs and strips) along with the utilization of STS on the rotational behaviour, charring rate, fire resistance time and failure mode of the proposed hybrid mass timber beam-end connection configurations.
Developing climate-responsive passive strategies for residential envelopes in the warm humid climate of South India
Authors: Vishnubhotla Lakshmi Visakha , Shanmugam Sornambiga , Tadepalli Srinivas 
Published: January 2022

PurposeEnergy codes for residential buildings in India prescribe design guidelines for each climate zone. However, these guidelines are broad and similar for different cities under the same zone overlooking climatic variations due to altitude, location and other geographical factors.Design/methodology/approachTo develop strategies addressing the city-specific requirements, a stepwise simulation approach was used. Integrated Environmental Solutions–Virtual Environment (IES-VE) was used to create a prototype of a singly detached residence. The applicability of strategies is studied during the day and night times. Optimum orientation, the thickness of insulation, Window–Wall Ratio, the impact of cross-ventilation and shading depth are determined for two cities – Tiruchirappalli and Coimbatore under the warm-humid climate zone of India.FindingsResults indicate that optimum insulation thickness and WWR vary between both cities during daytime and night time. In Tiruchirappalli, roof and wall insulation using polyurethane board (100 mm) and foam concrete (25 mm) offers a maximum reduction of 2.2°C indoors. Foam concrete (25 mm) insulation for roof and expanded polystyrene (25 mm) for walls reduce a maximum of 2.6°C during daytime in Coimbatore. Further, night ventilation with 20% WWR allows an average decrease of 0.5–0.6°C in triply exposed spaces facing the South. The use of a 2'0" depth shading device shows a maximum reduction of 0.1–0.3°C.Originality/valueThe contribution of this work lies in developing city-specific inputs presenting the advantage of easy replicability for other cities in the Indian context.