bluuebunny/crossref_metadata_2025_split huggingface dataset:
Condyloderes chidiyatapuensis sp. nov. (Kinorhyncha, Cyclorhagida, Centroderidae) from off Port Blair Andaman and Nicobar Islands, India.
Authors: Anand Arunima , PM Mohan 
Published: June 2022

Abstract
One new Kinorhyncha species, Condyloderes chidiyatapuensis sp. nov., is described in Andaman and Nicobar Islands, India. The same genus was reported fifty years ago in India. This species was identified based on specific characteristics such as cuspidate spines in segment 2,4,8,9, a pair of the laterodorsal spine in the 10th segment in the male specimen, the presence of lateral accessory spine on segment 8, ventromedial sensory spots and smaller mid-terminal spine. Similarly, the largest segment (9th segment) also has a cuspidate spine, which is unique in this species.
GATA6 impairs pancreatic cancer cells stemness by modulating Wnt/β-catenin signaling
Authors: Peng Yi , Gong Weiqi 
Published: June 2022

Abstract
Background: Cancer cells' stem cell capabilities are thought to have a role in the beginning and development of pancreatic ductal adenocarcinoma (PDAC). The Wnt/β -catenin signalling pathway is an important regulator of cancer cell stemness.Methods: In this article, through a small range of genetic screening, we found GATA Binding Protein 6 (GATA6), It belongs to a family of transcription factors that attach to the (A/T)GATA(A/G) consensus pattern to stimulate or repress gene expression. It was discovered to be overexpressed in certain PDAC patients with a good prognosis. The biological activities of GATA6 in pancreatic cancer, however, are unknown. In current investigations, we used western blotting assays, qPCR assays, Dual-luciferase reporter assays and Chromatin immunoprecipitation assays in combination with various cytological experiments to explore the molecular mechanisms involved.Results: Overexpression of GATA6 suppresses pancreatic cancer cell development both in vitro & in vivo by affecting stemness & downregulating the expression of stemness-related markers CD133, ALDH, CD44, & SOX-9, according to the findings. GATA6 upregulates the expression of stemness-related genes by inhibiting Wnt/β-catenin signalling & suppressing β-catenin nuclear translocation.Conclusion: GATA6 inhibits the stemness features of PDAC cells via the Wnt/β-catenin signalling pathway, according to this study. As a result, it might be a viable therapeutic target for cancer therapy.
Aggressive Driving, Rule-Breaking, and Driver Stress in Spanish-Speaking Countries
Authors: Dorantes-Argandar Gabriel , Barrales-Díaz Claudio Rodolfo , Ferrero-Pastor Elia Sigrid , Madrigal-Arroyo Minor David , Maegli-Quezada María Andrée , Marroquín-Gracias Lía Pamela , Tortosa-Pérez Macarena , Villalobos-Ruano Paola Alejandra 
Published: June 2022

Abstract
Driver stress is a phenomenon many have studied in all five continents. It has been the focus of curiosity for all sorts of disciplines, and science has been unable to curb it, much less park it. Driver Stress, Road Aggression and Road Rule-Breaking are crucial elements in traffic psychology. Those who do not respect the rules are a major factor in increased risk and major traffic accidents. Little work has been done in how these phenomena behave in Spanish-speaking countries, much less the development of useful instruments to evaluate them. This study aims to generate two unique scales that can be used in Spanish speaking countries regardless of culture or geography, as well as determining the influence Driver Stress has on Driver Aggression and Rule-Breaking. A sample of 1827 drivers from Mexico, Guatemala, Costa Rica, Chile and Spain was comprised. Through this paper, a small yet significant relationship was found between Driver Stress, Driver Aggression, and Driver Rule-Breaking. This suggests that personality factors are at play in these relationships in Spanish-speaking countries, and that these variables are an important factor in how drivers behave. This study also delivers two useful tools for evaluating Driver Aggression (LatinCAT) and Driver Rule-Breaking (LatinDANT) that have excellent construct validity for further research in Spanish-speaking countries.
Monoclonal Antibody Treatment of COVID-19 in a Pregnant Woman with Common Variable Immunodeficiency
Authors: Aberumand Babak , Betschel Stephen , Kamal Ramy , McKinney Brock 
Published: June 2022

Abstract
BackgroundSince the first reported case of COVID-19, infections due to the virus have ranged from mild to severe. Patients with primary immunodeficiency are thought to be at increased risk for infections such as COVID-19 due to the nature of their disease and being immunocompromised. Similarly, pregnant women by nature of physiological changes in immunity are susceptible to infections and consequently are felt to be at greater risk of contracting COVID-19 with potential grave consequences for not only the mother but also the fetus. Early treatment with novel therapeutics against the sars-cov-2 virus to prevent progression and these complications is paramount.Case PresentationA 31-year-old woman with a 22-year history of common variable immunodeficiency on subcutaneous immunoglobulin replacement therapy and 24 weeks pregnant with her third child presented to the Emergency Department with two-day history of pharyngitis that progressed to include nasal and chest congestion, non-productive cough and shortness of breath. Her vitals indicated temperature of 35 degrees Celsius, heart rate of 109 beats per minute, blood pressure 142/92 mmHg, respiratory rate 22/min and an oxygen saturation of 99% on room air. A workup was done and she was found to be positive for sars-cov-2 virus confirmed by PCR. She had a close contact, her husband, who had tested positive a few days prior. She had been previously vaccinated with three doses of the Moderna COVID-19 (Spikevax ®) vaccine. As she met the criteria for monoclonal antibody treatment, she received Sotrovimab on the same day of testing positive and tolerated it well with no side-effects. Her symptoms resolved within two to three days.ConclusionOur case, is the first to our knowledge, of a pregnant patient with common variable immunodeficiency diagnosed with COVID-19 and symptomatic successfully receiving treatment with Sotrovimab. Her rapid resolution of symptoms makes the use of monoclonal antibodies such as Sotrovimab a safe and useful option in this unique population.
Molecular intrinsic subtypes, genomic and immune landscapes of BRCA-proficient but HRD-high ER-positive/HER2-negative early breast cancers
Authors: Ladoire sylvain , Ballot Elise , Galland Loïck , Mananet Hugo , Boidot Romain , Arnould Laurent , Desmoulins Isabelle , Mayer Didier , Kaderbhai Courèche , Ilie Sylvia , Hennequin Audrey , Bergeron Anthony , Derangère Valentin , Truntzer Caroline , Ghiringhelli Francois 
Published: June 2022

Abstract
Purpose: The vast majority of research studies that have described the links between DNA damage repair or homologous recombination deficiency (HRD) score, and tumor biology, have concerned either triple negative breast cancers or cancers with mutation of BRCA1/2. We hypothesized that ER+/HER2- early breast tumors without BRCA1/2 mutation could have high HRD score, and aimed to describe their genomic, transcriptomic, and immune landscapes. Patients and methods: In this study, we reported BRCA1/2 mutational status, HRD score and mutational signature 3 (S3) expression, in all early breast cancer (eBC) subtypes from the TCGA database, with a particular focus in ER+/HER2-. In this subtype, bioinformatics analyses of tumor transcriptomic, immune profile, and mutational landscape, were performed, according to HRD status. Overall survival (OS), progression free-interval (PFI), and variables associated with outcome were also evaluated. Results: Among the 928 tumor samples analyzed, 46 harboured BRCA 1/2 mutations, 606 were ER+/HER2- (of which 24 were BRCA 1/2 mutated). We found a subset of BRCA-proficient ER+/HER2— eBC, with high HRD score. These tumors displayed significantly different immune, mutational, and tumor molecular signatures landscapes, compared to BRCA-mutated and BRCA-proficient HRD-low tumors. Outcome did not significantly differ between these 3 groups, but biological factors associated with survival are not the same across the 3 entities. Conclusion: This study highlights possible novel biological differences among ER+/HER2- breast cancer related to HRD status. Our results could have important implications for translational research and/or the design of future clinical trials, but require prospective clinical evaluation.
Succession and determinants of the early life nasopharyngeal bacteriome in a South African birth cohort
Authors: Claassen-Weitz Shantelle , Gardner-Lubbe Sugnet , Xia Yao , Mwaikono Kilaza S , Mounaud Stephanie Harris , Nierman William C. , Zar Heather J. , Nicol Mark Patrick , Workman Lesley 
Published: June 2022

Abstract
Background: Bacteria colonizing the nasopharynx play a key role as gatekeepers of respiratory health. Yet, dynamics of early life nasopharyngeal (NP) bacterial profiles remain understudied in low-and-middle-income countries (LMICs), where children have a high prevalence of risk factors for lower respiratory tract infection. We investigated longitudinal changes in NP bacterial profiles, and associated exposures, among healthy infants from low-income households in South Africa.Methods: We used short fragment (V4 region) 16S rRNA gene amplicon sequencing to characterise NP bacterial profiles from 103 infants in a South African birth cohort, at monthly intervals from birth through the first 12 months of life and six-monthly thereafter until 30 months. Results:Corynebacterium and Staphylococcus were dominant colonizers at one month of life, however these were rapidly replaced by Moraxella- or Haemophilus-dominated profiles by four months. This succession was almost universal, and largely independent of a broad range of exposures Warm weather (summer), lower gestational age, maternal smoking, no day-care attendance, antibiotic exposure, or low height-for-age z score at 12 months were associated with higher alpha and beta diversity. Summer was also associated with higher relative abundances of Staphylococcus, Streptococcus, Neisseria or anaerobic gram-negative bacteria, whilst spring and winter were associated with higher relative abundances of Haemophilus or Corynebacterium, respectively. Maternal smoking was associated with higher relative abundances of Porphyromonas. Antibiotic therapy (or isoniazid prophylaxis for tuberculosis) was associated with higher relative abundance of anerobic taxa (Porphyromonas, Fusobacterium and Prevotella) and with lower relative abundances of health associated-taxa Corynebacterium and Dolosigranulum. HIV-exposure was associated with higher relative abundances of Klebsiella or Veillonella and lower relative abundances of an unclassified genus within the family Lachnospiraceae. Conclusions:In this intensively sampled cohort there was rapid and predictable replacement of early profiles dominated by health-associated Corynebacterium and Dolosigranulum with those dominated by Moraxella and Haemophilus, independent of exposures. Season and antibiotic exposure were key determinants of NP bacterial profiles. Understudied but highly prevalent exposures prevalent in LMICs, including maternal smoking and HIV-exposure, were associated with NP bacterial profiles.
The frequency and risk factors of major complications after thermal ablation of liver tumours in 2084 ablation sessions
Authors: Huang Qian-nan , Pang Meng-ya , Zeng Qing-jing , He Xu-Qi , Zheng Rong-qin , Ge Mian , Li Kai 
Published: June 2022

Abstract
Background: To assess the frequency of major complications after thermal ablation of liver tumours and to determine risk factors for adverse events.Methods: A retrospective study was conducted between January 2015 and January 2021. A total of 2084 thermal ablation sessions in 1592 patients with primary and metastatic liver tumours were evaluated. The frequency of major complications was evaluated according to the Society of Interventional Radiology Standards, and putative predictors of adverse events were analysed using simple and multivariate logistic regression.Results: Thermal ablation-related mortality was 0.1% (2/2084), with an overall major complication rate of 5.6% (117/2084). The most frequent major complication was symptomatic pleural effusion (51.3%, 60/117). Multivariate logistic regression analysis revealed that a total maximum diameter of lesions >3 cm, microwave ablation (MWA) and MWA combined with radiofrequency ablation, intrahepatic cholangiocarcinoma and postoperative systemic inflammatory response syndrome were independent prognostic factors for major complications. Conclusions: Thermal ablation of liver tumours is a safe procedure with an acceptable incidence of major complications. The risk factors identified in this study will help to stratify high-risk patients.
A Study on the Optimum Design of Fuzzy Logic Control using Differential Evolution Algorithms for Omnidirectional Mobile Robot Navigation
Authors: Abdelwahab Mohamed , Parque Victor , Bab Ahmed M. R. Fath El , Abouelsoud Ahmed 
Published: June 2022

Abstract
Goal-oriented mobile robot navigation is significant for exploration, transport, and telerobotics ubiquitously. Among the existing mobile robot configurations, the omnidirectional platforms offer enhanced maneuverability and flexibility in navigation. Also, Fuzzy Logic (FL) is favorable regarding intelligible control rules and robustness to uncertainties among the existing control schemes. However, there exists a gap in the control of omnidirectional robots using FL, especially tackling stagnation and local-minima in goal-oriented navigation. This study presents a methodological framework, first of its kind to the best of our knowledge, combining the benefits of a behavioral FL and Differential Evolution for stagnation-free and goal-oriented omnidirectional robot navigation. Whereas FL considers a behavioral modulation between goal-seeking and obstacle avoidance, relevant classes of Differential Evolution (DE), which comprise exploration, exploitation, and self-adaptation modalities, tackle FL membership functions' search space. Through rigorous computational experiments, we demonstrate that Rank-Based Differential Evolution (RBDE)'s exploitative features show favorable performance in learning and tackling stagnation in the context of a small number of function evaluations (up to 1000) and unknown/partially known environments, both of which are relevant for quick adaptation to dynamic environments. After explaining the reason for learning efficiency and navigation performance in training-testing scenarios, we discuss the potential of rank-based learning algorithms.
Premature Rupture Of Membrane and Its Associated Factors Among Pregnant Women Admitted in Dire Dawa Hospitals, in Mid COVID-19 Pandemic in Ethiopia
Authors: Tolera Shume , Ibrahim Neil Abdurashid , Sema Alekaw , Sedeta Bezabih Amsalu 
Published: June 2022

Abstract
Background: Premature rupture of membranes is the spontaneous leakage of amniotic fluid from the amniotic sac that occurs at least one hour before the onset of labor. It is a common complication of pregnancy that leads to neonatal and maternal morbidity and mortality. There is little evidence of the prevalence and associated factors among pregnant women admitted to the obstetrics ward in the study area..Objective: To assess the prevalence and associated factors among pregnant women admitted to Dire Dawa hospitals in eastern Ethiopia.Method: A facility-based cross-sectional study was conducted from May 17 to June 17, 2021. A systematic random sampling technique was used to select 392 pregnant women admitted to an obstetric ward. Data was collected using an interviewer-administered questionnaire and checklist to obtain data from the medical record. Data was collected with Kobo tool software and exported to SPSS version 24. The crude odds ratio and the AOR with 95% CI were calculated to assess the association among variables and control the confounding factors. The level of significance was declared at a p-value < 0.05. Result: All 392 (100%) pregnant women were included in the study. The prevalence of PROM was found to be 22.4%. Pregnant women who had a history of previous preterm labor (AOR = 2.449, 95% CI: 1.422, 4.217), history of PROM (AOR = 2.663, 95% CI: 1.567, 4.526), history of anemia (AOR = 2.497, 95% CI: 1.371, 4.550), history of UTI (AOR = 2.715, 95% CI: 1.575, 4.679) and history of chewing khat (AOR = 1.936, 95% CI: 1.055, 3.552) were significantly associated with pre-labor rupture of membrane.Conclusion: The prevalence of pre-labor rupture of membranes in the study was high when compared to previous studies. An intervention that focuses on strengthening the integration of messages on health promotion and disease prevention, routine early screening, diagnosis and quick treatments of UTI and anemia, nutritional counseling, iron supplementation, and giving information regarding substance use during pregnancy should be recommended.
Identification, purification, characterization and biopreservation potential of antimicrobial peptide of Pediococcus acidilactici NCDC 252
Authors: DHANDA SHWETA , Kumar Parveen , Bansal Poonam , Dhanda Suman 
Published: June 2022

Abstract
Lactic acid bacteria (LAB) are economically important because of their fermentative aptitude and also confer health and nutritional benefits. Pediococcus acidilactici NCDC 252 is potential probiotic with novel genome that exhibited all in-vitro probiotic attributes, colonization in intestine and survival in conditions that mimick gastric, intestinal and vaginal fluid. Genome was screened for its ability to produce antimicrobial peptide (AMP). AMP activity was screened by spot on lawn method and by measuring zone of inhibition (ZOI) against indicator strain (E.coli). AMP production was optimized with highest production in MRS broth at 32℃, pH 6 and after 36 h of incubation under both aerobic and anaerobic conditions. AMP was purified 2.2 fold with yield of 7.2% and specific activity of 64.5 U mg/ml by ammonium sulphate precipitation (upto 70%) and gel filtration chromatography. Molecular weight of purified AMP was calculated to be 2.5 KDa. Tricine SDS-PAGE and LC/MS studies confirmed it to be a low molecular weight peptide. AMP was stable over pH range of 3 to 9 and temperature range of 40℃ to 100℃. Enzymatic treatment inactivated the AMP whereas, detergent treatment enhanced AMP’s activity. Biopreservation potential of purified AMP and whole NCDC 252 cells was studied by incubating them with some dairy products, apple juice, apricot pulp and pre-pasteurized wine over 72 h. Bacterial growth in AMP treated food products reduced significantly after 72 h. Viable cell count decreased to approximately half in all studied food items in both purified AMP treated and NCDC 252 treated food products.
Student Survey After Ten Years of Continuous Blended Teaching of Echocardiocraphy
Authors: Parra Víctor M. , Fita Guillermina , Azqueta Manel , González Mauricio , Aranda Fernando , Maestre MaLuz , Silva Jorge , Hortal Javier , Morales Daniel , Bórquez Emiliano , Adasme Fabián , Real María Isabel , Mercadal Jordi , Zelada Pamela , Riobó Gonzalo , Galdames Katia , Domenech Raúl J. 
Published: June 2022

Abstract

Aims: The aim of this study is to analyze the impact of 10 years of blended learning in echocardiography.Methods and results: A total of 140 of 210 medical doctors who graduated from our teaching program from 2011 to 2020 responded to the survey. Among them, 53,57% were anesthesiologists and 26,42% intensivists. More than 85% of respondents indicated that the online period met their expectations, and 70,2% that the hands on practice met the objectives. Posterior to the Diploma there was a significant increase in the frequency of use of echo compared to before the Diploma, both in transthoracic and transesophageal echocardiography, from 24,29% to 40,71% and from 13,57% to 27,86%, respectively. The echo was applied mainly in the perioperative period (56,7%) and in intensive care (32,3%), and only in 11% of cases it was used in emergency units. Additionally, 92,4% of respondents revealed that it was very useful in their subsequent professional practice and 92,47% that it helped them in the interpretation of echo reports.Among the strengths they highlighted ‘the theoretical content, clinical cases, hands on activity and the teaching staff’. Among the weaknesses, 50% requested a practice period lasting more than one week.Conclusion: The blended learning of echocardiography over a decade has been satisfactorily evaluated by the graduated specialists and has been related to a significant increase in its use in the perioperative period and in intensive care. The main challenges are to establish a longer period of practice and achieve greater scope in Emergency Medicine.
Simulation and evaluation of the effect of vitamin C on the proline amino acid and glycine collagen in athletes' muscles
Authors: Raouf Mahmoud , Mahdavian Leila 
Published: June 2022

Abstract
Vitamin C plays an important role in the amino acid metabolism of athletes, especially the formation of collagen. Collagen is the main constituent of connective tissue that holds bones and muscles together and It is the main and most abundant protein in the body that prevents the weakening of bones, muscles and skin wrinkles. The amino acids present in the structure of collagen include glycine, proline, arginine and hydroxyproline. In this study, the interaction of amino acids glycine and proline in the presence of vitamin C was simulated and evaluated by B3LYP/6-311++G (p, d). The results show that the amino acids sequence in the presence of vitamin C has more orientation and order and prevents the formation of other chains. The results of the mechanism of conversion of the proline amino acid to hydroxyproline are investigated and all its structural and thermodynamic parameters are obtained.
Effectivity of Hands-on Near-peer teaching in Scientific Writing for Indian Medical Students
Authors: Puri Oshin , Unnithan Vishnu , Kumar Pratyush , Reddy Asmitha P , Pahuja Vrinda , Ganla Aakruti , Paul Aritra , Vaghela Gladson , Bhatia Mannat Kaur , Khurana Navpreet , Rangan Rahul , Singh Ripudaman , Pathania Monika 
Published: June 2022

Abstract
Purpose: This study focuses on assessing the effectiveness of near-peer teaching, small group discussions, and hands-on assignments in enhancing the scientific writing knowledge & skill of a cohort of Indian medical students.Method: 175 medical students were randomly selected from a 516 membered FORLER (Fundamentals of Research Learning and Educational Reform) interest cohort for hands-on near-peer training in medical scientific writing. The participants attended weekly near-peer lectures and participated in biweekly in-group discussions over 5 weeks focused on Narrative Reviews, Original Articles, Case Reports, Short Communications, & Systematic Reviews. Participants were regularly evaluated following Kirkpatrick’s model of evaluating training.Results: N=135 medical students aged 20 ± 1.3 yrs (17-23 yrs); 56.29% females completed all the assignments in FORLER. Amotivation decreased & attitude towards research improved significantly (p<0.05) after the workshop. The theoretical understanding of the students of all 5 forms of scientific writing improved significantly. A significant (p=0.047) moderate direct (P=0.639) correlation was also observed between the increase in confidence and overall increase in test scores. Post-FORLER manuscripts were scored significantly (p<0.05) higher (by expert double-blind reviewers) than pre-FORLER manuscripts in all forms of scientific writing except Case reports.Conclusion: A combination of near-peer teaching, small group discussions, and manuscript writing exercises lead to a statistically significant increase in scientific writing knowledge and skills. Higher confidence levels were reported after the training initiative. A decrease in amotivation was also observed with participants favoring integration of similar initiatives into the undergraduate training framework.