bluuebunny/crossref_metadata_2025_split huggingface dataset:
Micropropagation and in Vitro Flowering in Basella Spp.
Authors: Ranganatha Manjula , RAO NAGASHREE N , Giridhar Parvatham , sharma ashwani 
Published: November 2022

Abstract
Basella spp. a perennial vine of Basellaceae family used as a leafy vegetable. Phytonutrient of Basella spp. is being exploited in Indian medicinal system since antiquity for its antifungal, anticonvulsant, anti-inflammatory, antipyretic, antiulcer and analgesic properties. Propagation of Basella through seeds have limitations for germination and flowering, in vitro regeneration was studied on MS (Murashige& Skoog) media supplemented with various plant growth regulators, indole acetic acid (IAA), Indole butyric acid (IBA), 1-naphthalene acetic acid (NAA), N,6-benzyladenine (BA), kinetin (KIN), zeatin (ZEA), gibberillic acid (GA3), and adenine sulphate (ADS), silver nitrate (AgNO3) as additives. The shoot bud initiation was observed in all the combinations studied showing a good response for direct regeneration. Shooting (84%) was observed in 12 days after inoculation in 1mg l− 1 BA + 0.1 mg l− 1 NAA. Liquid media containing 0.1mg l− 1 BA + 0.5 mg l− 1 KIN + 0.1 mg l− 1 IAA was preeminent in multiple shoots (22 ± 0.13) production with average shoot length (5.81 ± 0.19) in 5 weeks (wk). Supplementation of 40 mg l− 1 AgNO3 and 40 mg l− 1 ADS to media containing 1mg l− 1 BA + 0.1 mg l− 1 NAA resulted in enhanced number of elongated shoots with number of leaves. In vitro flowering was obtained on MS media containing 0.5mg l− 1 BA + 0.5 mg l− 1 GA3 concentrations. The survival rate of hardened plants was 90% after transferring to soil. This protocol can be efficiently used for mass production for regeneration of genetically transformed Basella spp. in studying its metabolite profile specially betalains and transformation of Basella.
Identification of early diagnosis markers of pancreatic ductal adenocarcinoma (PDAC) using publicly available transcriptomic tumor and blood sample data
Authors: Sionakidis Aristeidis , Lalagkas Panagiotis Nikolaos , Malousi Andigoni , Vizirianakis Ioannis S. 
Published: November 2022

Abstract
Pancreatic ductal adenocarcinoma (PDAC) is the most frequently diagnosed form of pancreatic cancer worldwide. PDAC is associated with poor survival rate mainly due to the disease being usually diagnosed at late stages. Publicly available gene expression data from 10 studies with tumour tissue (448 samples) and/or blood samples (128 samples) from PDAC patients were pooled together and analysed for the identification of stage-specific (American Joint Committee for Cancer, AJCC staging) and global diagnostic markers. Validation of markers was performed using Cancer Genome Atlas (TCGA) PDAC expression data. Differential gene expression analysis was carried out to compare tumour and normal samples (stage-specific tissue samples vs. normal tissue samples and stage-agnostic blood samples vs. normal blood samples). Active subnetwork search and miRNA enrichment analysis were used to identify enriched gene networks and miRNA interactions. We identified 820 consistently deregulated genes in tissue samples of all stages and blood samples. Active subnetwork analysis revealed enriched ribosome, proteasome, adherens junction and cell cycle pathways across all stages and blood samples suggesting biological plausibility. Stage-specific enriched miRNAs with diagnostic potential were also identified (miR-21, miR-29, miR-124, miR-30, for stages 1-4 respectively). Extensive gene expression deregulation was found in all tumor stages with significant overlap. Additionally, miRNA contribution to PDAC pathology may be important and probably mediated by distinct miRNAs in each stage of PDAC. We therefore present a list of markers and miRNAs that could potentially act as a diagnostic tool for early detection of PDAC onset.
Spatial-temporal characteristics of geological disaster resilience in poverty and disaster-prone areas in China: A case study of Ganzi Prefecture
Authors: Zhou Ying , Li Yulian , Zhang Luyun , Su Qihao , Huang Xing 
Published: November 2022

Abstract
Research on resilience under the influence of geological disasters in poverty and disaster-prone areas is a valuable exploration of disaster prevention and mitigation. Ganzi Prefecture occurs geological disasters frequently, resulting in loss of life, destruction of property, and harm to other infrastructure. Applying resilience can improve the ability to absorb and resolve risks, providing insights into sustainable development in Ganzi. This paper aims to assess the resilience of Ganzi Prefecture based on the widely used Baseline Resilience Indicators for Community. This modified system consists of 22 indicators divided into four dimensions: society, economy, infrastructure, and environment. The changes in disaster resilience for 18 nations in Ganzi are calculated from 2011 to 2020. Moran's I model is used to synthesize the dynamic evolvability and spatial correlation of disaster resilience. Geodetector is used to identify primary factors influencing disaster resilience. The findings reveal that (1) disaster resilience increased continuously in Ganzi during the period between 2011 and 2019, and that the gap between the disaster resilience of various counties gradually shrank. (2) The spatial distribution of disaster resilience in Ganzi varies significantly. Southeast Ganzi has greater resilience, whereas the northwest region has a considerably lower resilience. (3) The article identifies seven critical determinants of disaster resilience, and interaction factors have a significant impact on the outcomes. Based on these findings, this study proposed local regional sustainable development policy implications.
Prediction of type 2 diabetes mellitus using hematological factors based on machine learning approaches: A cohort study analysis
Authors: Mansoori Amin , Sahranavard Toktam , Hosseini Zeinab Sadat , Soflaei Sara Saffar , Emrani Negar , Nazar Eisa , Gharizadeh Melika , Khorasanchi Zahra , Ghamsary Mark , Ferns Gordon , Esmaily Habibollah , Ghayour-Mobarhan Majid 
Published: November 2022

Abstract
Background
Type 2 Diabetes mellitus (T2DM) is a significant public health problem globally. The diagnosis and management of diabetes are critical to reduce the complications of diabetes including cardiovascular disease and cancer. This study was designed to assess the potential association between T2DM and several routinely measured hematological parameters.
Method
This study was a subsample of 9000 adults aged 35–65 years recruited as part of Mashhad stroke and heart atherosclerotic disorder (MASHAD) cohort study population. Data mining techniques including logistic regression (LR), decision tree (DT) and bootstrap forest (BF) algorithms were applied to analyze data. All data analyses were performed using SPSS version 22 and SAS JMP Pro version 13 at a significant level of 0.05.
Results
Based on the performance indices, the BF model gave high accuracy, precision, specificity, and AUC compared to the other models. Thus, according to all the assessed hematological factors, the most effective risk factors for predicting the development of T2DM in the BF model were age and WBC.
Conclusion
In summary, the BF model represented a better performance to predict T2DM. Also, our selected model provides valuable information on critical determinants to predict T2DM like age and WBC.
Selecting a surgical approach for incurable tuberculous thoracolumbar kyphosis and analyzing its clinical efficacy
Authors: Maihemuti Maierdanjiang , Dai Zhibing , Sun Yachao , Maimaiti Aierpati , Mamat Mardan , Yang Wenpeng , Wu Junshen , Kasim Mamatali , Yasen Abudukadier , Jiang Renbing 
Published: November 2022

Abstract
Background: Incurable spinal tuberculosis is extremely uncommon, its treatment is challenging and still controversial. We aimed to investigate the efficacy of posterior and combined posterior-anterior surgical approaches for incurable tuberculous thoracolumbar kyphosis.
Materials and methods: We retrospectively analyzed 63 cases of incurable tuberculous thoracolumbar kyphosis. The mild group was treated with posterior surgery alone, and the severe group was treated with the combined posterior-anterior surgery. The surgical efficacy was evaluated based on the clinical symptoms, Cobb angle correction rate, SVA, operative time, intraoperative bleeding, and surgical complications. Symptoms and function were assessed using the American Spinal Injury Association (ASIA) spinal cord injury classification, the visual analogue scale (VAS), the Oswestry dysfunction index (ODI), and the Kirkaldy-Willis functional score. The degree of implant fusion was evaluated according to the Eck fusion grading scale.
Results: The preoperative Cobb angles were 59.4°±12.6° and 102.9°±16.6°, and the improved postoperative Cobb angles were 19.9°±6.2° and 28.5°±9.6° for the mild and severe groups, with correction rates of 65.5%±12.0% and 72.0%±9.5%, respectively. The Cobb angle losses were 3.1°±1.4° and 4.2°±1.7° at the last follow-up for the mild and severe groups, respectively. The preoperative SVA were 27.6±10.7 mm and 39.1±18.6 mm, which postoperatively improved to 20.6±9.0 mm and 26.4±12.1 mm in the mild and severe groups, respectively. All patients had an ASIA classification of E, except two patients in the severe group with a classification of D at the time of the final follow-up. All patients with bone grafting achieved grade I fusion. The Kirkaldy-Willis functional scores were 89.7% and 85.3% for the mild and severe groups, respectively, except for two case (1 mild and 1 severe group)with broken rods. no tuberculosis recurrence, internal fixation loosening, breakage, and obvious loss of correction were found during the follow-up period.
Conclusion: Simple posterior surgery can achieve the desired clinical outcomes in patients with mild non-curative tuberculous thoracolumbar kyphosis. However, in severe patients with accumulated multiple segments, combined posterior-anterior surgery is required for satisfactory deformity correction, complete lesion removal, and significant bone grafting support, making it a safe and reliable treatment method.
Level of evidence: IV
Pan-cancer analysis of the oncogenic effect of SorCS1 in human tumors and its correlation with LRP2 protein
Authors: Deng Yuqing , Zhang Xiaoyuan , Li Dongliang , Xu Haiyan 
Published: November 2022

Abstract
Background: SorCS1 is a member of the mammalian type –Ⅰtransmembrane receptor family containing Vps10p structural domain. According to known results, SorCS1 plays important role in ligands absorption and trafficking, and is related to many nervous diseases. LRP2 is low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor. Up to date, more and more emerging cellular or animal-based evidence supports a relationship between SorCS1 and cancer; in the meanwhile, SorCS1 shows some correlation with LRP2, bust there is no relevant analysis data showing how SorCS1 works with other factors and LRP2. Therefore, we explored the potential oncogenic role of SorCS1，and correlation with LRP2 in a variety of cancers using the TCGA (The Cancer Genome Atlas) and GEO (Gene Expression Omnibus) databases.
Methods: The related data of human tumor patients were obtained from the TCGA and GEO databases, respectively. The TIMER and GEPIA2 database was used to analyze the expression of SorCS1 and LRP2 in tumors and their survival analysis. The UALCAN database was used to analyze the differences in the expression of SorCS1 and LRP2 in tumor staging. Then, the TIMER database was used for immune cell infiltration analysis, and the GEPIA2 database was used for the detection of genes associated with the SorCS1 gene and for enrichment analysis.
Results: According to current studies, SorCS1 expression is usually lowly in most cancers but indeed is significantly correlated with the prognosis in several ones. In latter, our analyses showed a significant correlation between SorCS1 and LRP2, such as SKAM (Stomach adenocarcinoma), TGCT (Testicular Germ Cell Tumors), THCA (Thyroid carcinoma), UVM (Uveal Melanoma), and some other cancers. In the meanwhile, there was an increased extent of mutation in SorCS1. Our correlation analyses provide some understanding of the oncogenic role of SorCS1 and LRP2 in different tumors and their relevance. In addition, we detected correlations between SorCS1 and CD8+ immune cells or fibroblast infiltration in databases such as STAD. We counted potentially relevant genes for SorCS1 in various cancers and did KEGG and GO enrichment analyses of these genes, providing new ideas for the response pathways involved in SorCS1 in cancers.
Conclusion: SorCS1 was aberrantly expressed in most tumors and shared the same expression trend as LRP2.
Perceptions of professional nurses towards the inclusion of spiritual activities in oncology practice in the uMgungundlovu and eThekwini Health Districts, KwaZulu-Natal, South Africa
Authors: Sewkarran Vashni , Gumede Emelda Zandile 
Published: November 2022

Abstract
Background
Internationally the inclusion of spiritual activities has been well adopted into nursing care. The South African perspective highlights a huge gap regarding incorporating spiritual activities into nursing care
Method
A quantitative descriptive survey was used to describe the perceptions of nurses towards inclusion of spiritual activities in oncology practice. Purposeful sampling of eighty-nine participants that met the eligibility criteria was conducted. Questionnaires were used to collect data from four sites over a period of four weeks, followed by analysis using the SPSS, version 27.
Results
The findings indicated that 80% of professional nurses believed in God. Activities like arranging visits by chaplains, and showing kindness, cheerfulness and reassurance were highly rated., however, activities of art and creativity were poorly rated, 32.6%.
Conclusion
This study produced empirical evidence that spiritual care can be provided by including various activities in oncology practice to promote comprehensive nursing care. A module on spirituality should be included in the new oncology program to boost the nurse’s levels of confidence in spiritual care. The study will enhance the nurses’ awareness and expertise with providing a range of activities in different beliefs. Future research would benefit from expanding this research to other health professionals.
PD-L1 is Required for Estrogen-induced Protection Against Severe EAE in IL-10 Deficient Mice
Authors: Offner Halina , Lockwood Denesa , Meza-Romero Roberto , Vandenbark Arthur A. 
Published: November 2022

AbstractBackground IL-10 knockout (KO) mice can be protected against experimental autoimmune encephalomyelitis (EAE) with low-dose estrogen (E2) treatment similar to wild type (WT) mice, indicating that IL-10 is not required for E2-induced EAE protection. Our previous study demonstrated that E2 treatment induced an increase in programmed death ligands 1 (PD-L1) and 2 (PD-L2) on monocytes and macrophages in the periphery and within the CNS. In this study, we selectively inhibited the function of PD-L1 and PD-L2 to evaluate their critical role in maintaining E2-induced protection against EAE in IL-10-KO mice. Methods This study used female IL-10 KO mice pre-treated with either E2 or sham pellets seven days prior to induction of EAE and subsequently treated with Vehicle or antibodies to PD-L1, PD-L2 or respective isotype controls. Mice were scored daily for EAE severity over 21 days post-immunization for EAE induction. Cells from the spleen and brain were evaluated by flow cytometry. Results Differences in EAE severity were assessed in E2 and sham pre-treated IL-10-KO mice treated with α-PD-L1 or α-PD-L2 antibodies over the course of disease compared to treatment with Vehicle or anti-isotype antibodies. The results revealed real-time development of severe EAE in E2-pre-treated IL-10-KO mice treated with α-PD-L1 but not α-PD-L2 antibodies, mediated in part by increased percentages of activated CD74+CD11b+myeloid cells in spleen and brain as well as splenic B-cells, T-cells and CD73+cells. Conclusion These results demonstrate unequivocally that PD-L1 but not PD-L2 was required to retain the inhibitory effects of E2 on clinical EAE scores in female IL-10-KO mice and further implicate the emergence of the MIF/CD74 axis as a contributing pathogenic mechanism.
WITHDRAWN: Heavy metal enrichment characteristics of soil in manganese mining wetland by Philonotis thwaitesi
Authors: Wu Dongyi , Zhang Zhaohui , Wang Zhihui 
Published: November 2022

Abstract
Open pile manganese ore generates heavy metal pollution, which affects the surrounding environment, and it is necessary to monitor and remediate heavy metals in the mine area.
In this study, we investigated the correlation between the accumulation of heavy metals in Philonotis thwaitesii and the content of heavy metals in soil by investigating the characteristics of heavy metals in Philonotis thwaitesii, Spirogyraand soil in the wetland around the manganese mine area in Zhaiying Town, Songtao County, Guizhou Province，in order to provide a scientific basis for ecological restoration and soil heavy metal pollution management in manganese mine area.
The main results are as follows: the soil in the study area is moderately polluted by heavy metals and above; the correlation between heavy metals in Philonotis thwaitesiiis significant and influenced by soil; compared with Spirogyra, Philonotis thwaitesii has a stronger ability to enrich heavy metals; it is advantageous for remediation of moist polluted environment.
In conclusion, Philonotis thwaitesii has a certain degree of enrichment capacity for heavy metals, and can be used for the comprehensive treatment of heavy metal pollution in manganese mining sites, as well as the bioremediation of polluted areas associated with it. And the content of Mn in Philonotis thwaitesii is up to 11799mg/kg, whether it can be used as a manganese hyperaccumulator, further research is needed.
Fulvestrant plus palbociclib in advanced or metastatic hormone receptor-positive/human epidermal growth factor receptor 2-negative breast cancer after fulvestrant monotherapy: Japan Breast Cancer Research Group-M07 (FUTURE trial)
Authors: Watanabe Kenichi , Niikura Naoki , Kikawa Yuichiro , Oba Mari , Kobayashi Kokoro , Tada Hiroshi , Ozaki Shinji , Toh Uhi , Yamamoto Yutaka , Tsuneizumi Michiko , Okuno Toshitaka , Iwakuma Nobutaka , Takeshita Takashi , Iwamoto Takayuki , Ishiguro Hiroshi , Masuda Norikazu , Saji Shigehira 
Published: November 2022

Abstract
Purpose: The combination of cyclin-dependent kinase 4/6 inhibitors and endocrine therapy is a standard treatment for hormone receptor (HR)-positive/human epidermal growth factor receptor 2 (HER2)-negative metastatic breast cancer (MBC); however, their toxicities and financial burden are major issues, especially for prolonged treatment. We investigated fulvestrant plus palbociclib in patients with HR-positive MBC resistant to fulvestrant monotherapy.
Methods: Patients who initially received fulvestrant as their first- or second-line endocrine therapy were assigned to group A. Patients with disease progression during fulvestrant monotherapy who subsequently received fulvestrant plus palbociclib were assigned to group B. The primary endpoint was progression-free survival (PFS1) in group B. The secondary endpoints were PFS from the start of monotherapy (PFS2) in group A and the time to treatment failure (TTF) from treatment initiation.
Results: Between January, 2018 and February, 2020, we enrolled 167 patients in group A (January 2018–February 2020) from 55 institutions, of whom 72 subsequently received fulvestrant plus palbociclib and were enrolled in group B. The median follow-up was 23.8 and 8.9 months in groups A and B, respectively. The median PFS in group B (combination therapy) was 9.4 (90% confidence interval [CI]: 6.9–11.2) months (p <0.001). This was 25.7 (90% CI: 21.2–30.3) months in group A (fulvestrant monotherapy). The TTF in group B was 7.2 (90% CI: 5.5–10.4) months. In the post-hoc analysis the median PFS1 in group B among patients with longer-duration fulvestrant monotherapy (>1 year) was longer than that of patients with shorter-duration monotherapy (≤1year) (11.3 vs. 7.6 months). No new toxicities were observed.
Conclusion: Our findings suggest that palbociclib plus fulvestrant after disease progression despite fulvestrant monotherapy is potentially safe and effective in patients with HR-positive/HER2-negative advanced MBC.
Application of Magnetic Resonance Image Compilation (MAGiC) in the Diagnosis of Middle-Aged and Elderly Women with Osteoporosis
Authors: Chen Yiming , Mei Xiuting , Liang Xuqian , Cao Yi , Peng Cong , Fu Yang , Zhang Yulong , Liu Cuifang , Liu Yang 
Published: November 2022

Abstract
Objective: To investigate the feasibility of diagnosing osteoporosis (OP) in women through magnetic resonance image compilation (MAGiC).
Methods: A total of 110 patients who underwent lumbar magnetic resonance imaging and dual X-ray absorptiometry examinations were collected and divided into two groups according to age and bone mineral density: osteoporotic group (OP) and non-osteoporotic group (non-OP). The variation trends of T1 (longitudinal relaxation time), T2 (transverse relaxation time) and BMD(bone mineral density) with the increase of age, and the correlation of T1 and T2 with BMD were examined by establishing a clinical mathematical model.
Results: With the increase of age, BMD and T1 value decreased gradually, while T2 value increased. T1 and T2 had statistical significance in diagnosing OP (P=0.000), and there is moderate positive correlation between T1 and BMD values (R=0.636, P=0.000), while moderate negative correlation between T2 and BMD values (R=0.694, P=0.000). Receiver characteristic curve test showed that T1 and T2 had high accuracy in diagnosing OP (T1 AUC=0.982, T2 AUC=0.978), and the critical values of T1 and T2 for evaluating osteoporosis were 0.625s and 0.095s, respectively. Besides, the combined utilization of T1 and T2 had higher diagnostic efficiency (AUC=0.985). Combined T1 and T2 had higher diagnostic efficiency (AUC=0.985). Function fitting results of OP group: BMD=-0.0037* age -0.0015*T1+0.0037*T2+0.86, sum of squared error (SSE) =0.0392, and non-OP group: BMD=0.0024* age -0.0071*T1+0.0007*T2+1.41, SSE=0.1007.
Conclusion: T1 and T2 value of MAGiC have high efficiency in diagnosing OP by establishing a function fitting formula of BMD with T1, T2 and age.
Neuroprotective effect of a novel brain-derived peptide HIBDAP against oxygen-glucose deprivation through inhibition of apoptosis in PC12 cells
Authors: Jiang Chenhong , Hu Yina , Hou Xuewen , Qiu Jie 
Published: November 2022

Abstract
Background To investigate the effect of a novel brain-derived peptide HIBDAP (Hypoxic Ischemic Brain Damage Associated Peptide) on cell apoptosis after oxygen-glucose deprivation (OGD) in PC12 cells.
Methods The sequence of HIBDAP (HSQFIGYPITLFVEKER) was coupled with the carrier peptide of the transactivator of transcription (TAT) sequence (YGRKKRRQRRR). The FITC labeled TAT-HIBDAP was observed by the fluorescence microscope. After TAT-HIBDAP treatment and OGD treatment, PC12 cell apoptosis rate was analyzed using lactate dehydrogenase (LDH) leakage andAnnexin V-fluorescein isothiocyanate (FITC) assay. Mitochondrial membrane potential (ΔΨm) assay was examined by fluorescence microscope. Protein expressions of apoptosis factors were examined by Western blotting.
Results FITC-labelled TAT-HIBDAP could enter into PC12 cell nucleus. Compared with the OGD group, TAT-HIBDAP at low concentrations (1μM, 5μM, 10μM) significantly reduced the apoptosis rate of PC12 cells except the 20μM concentration, especially the 5μM concentration has the most obvious effect. There were remarkable increases of △Ψm after different concentrations (1μM, 5μM, 10μM, 20μM) of TAT-HIBDAP pretreatment and the 5μM concentration also has the most obvious effect. TAT-HIBDAP could rescue the increased ratio of Bax/Bcl-2 and Caspase-3 activation induced by OGD.
Conclusions TAT-HIBDAP is resistance to OGD-induced PC12 cells apoptosis through regulating the pathway of Bax/Bcl-2/Caspase-3, which may supply a novel therapeutic strategy for neonatal HIBD.