bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ_0000554 is identified as a cancer-promoting circRNA in colorectal cancer by regulating the miR-1205/LASP1 axis
Authors: Luo Jinlong , Yang Hua , Peng Xuefeng , Zhang Faqiang , Shu Shilong , Lan Ke , Tu Shengjin , Lu Kai , Cha Xiaoying 
Published: September 2022

Abstract
Background
Colorectal cancer (CRC) is a prevalent malignant tumor with poor prognosis. Circular RNAs (circRNAs) are key regulators in the progression of CRC. Our study aimed to disclose the role of circ_0000554 in CRC.

Methods
The expression of circ_0000554, miR-1205 and LIM and SH3 protein 1 (LASP1) was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, invasion and migration were monitored using cell counting kit-8 (CCK-8) assay, EdU assay, transwell assay and wound healing assay respectively. The protein levels of C-myc, matrix metallopeptidase 2 (MMP-2) and LASP1 were detected by western blot. Tumor formation assay in nude mice was conducted to explore the role of circ_0000554 in vivo. The association between miR-1205 and circ_0000554 or LASP1 was identified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.

Results
circ_0000554 was upregulated in CRC tissues and cells, high circ_0000554 expression was significantly linked to shorter overall survival. Downregulation of circ_0000554 restrained cell growth and metastasis while promoted apoptosis in vitro, and suppressed tumorigenesis of CRC in vivo. Furthermore, mechanism study and rescue experiments confirmed miR-1205 could be sponged by circ_0000554 and its inhibitor reversed the inhibitory effect of circ_0000554 silencing on CRC progression. LASP1 was a target gene of miR-1205 and the upregulation of LASP1 overturned miR-1205-induced effects on CRC cells. Circ_0000554 could elevate LASP1 expression via interacting with miR-1205.
Spatial behavior of red deer (Cervus elaphus) in Northern Apennines: are we managing them correctly?
Authors: Fontana Riccardo , Calabrese Licia , Lanzi Ambrogio , Armaroli Elisa , Raganella Pelliccioni Elisabetta 
Published: September 2022

AbstractBackgroundThroughout their range, red deer are a well-studied species. In Italy, this species occupies two ecologically different ranges: the Alps and the Apennines. Although several studies have described the spatial behaviour of red deer in the Italian Alps, no data are available for the Apennine population.ResultsThe spatial behaviours of 13 deer from the Northern Apennines range were analysed for the first time using GPS-GSM telemetry from 2011 to 2017. Red deer displayed two coexisting strategies, i.e., migratory and stationary. In our sample, females tended to migrate more than males. We found a high level of interindividual variability in the date of migration/return, while each migratory deer was very conservative during the study period. The migration ranges were on average 12 ± 4.2 km from the resident range. Both migratory and resident deer displayed high site fidelity. No switch from the migratory to stationary strategy was observed for any deer during the study period; however, the period could have been too short to detect any switch. At the management level, over 18 management cycles occurred during the study period, and a spatial mismatch was found between deer range and management units (districts) in 38.9% (7) of the cases. Merging the districts belonging to each province to obtain an area of approximately 1000 km2would partially address such spatial mismatch, reducing its occurrence to 22%.ConclusionsDespite the small sample size, these results can guide future management actions. However, an in-depth study with a larger sample size is required to better understand and manage the red deer Apennines population.
Ex vivo expanded human regulatory T cells modify neuroinflammation in a preclinical model of Alzheimer’s disease
Authors: Faridar Alireza , Vasquez Matthew , Thome Aaron D. , Yin Zheng , Xuan Hui , Wang Jing Hong , Wen Shixiang , Li Xuping , Thonhoff Jason R. , Zhao Weihua , Zhao Hong , Beers David R. , Wong Stephen T. C. , Masdeu Joseph C. , Appel Stanley H. 
Published: September 2022

AbstractBackgroundRegulatory T cells (Tregs) play a neuroprotective role by suppressing microglia and macrophage-mediated inflammation and modulating adaptive immune reactions. We previously documented that Treg immunomodulatory mechanisms are compromised in Alzheimer’s disease (AD). Ex vivo expansion of Tregs restores and amplifies their immunosuppressive functions in vitro. A key question is whether adoptive transfer of ex vivo expanded human Tregs can suppress neuroinflammation and amyloid pathology in a preclinical mouse model.MethodsAn immunodeficient mouse model of AD was generated by backcrossing the 5xFAD onto Rag2 knockout mice (5xFAD-Rag2KO). Human Tregs were expanded ex vivo for 24 days and administered to 5xFAD-Rag2KO. Changes in amyloid burden, microglia characteristics and reactive astrocytes were evaluated using ELISA and confocal microscopy. NanoString Mouse AD multiplex gene expression analysis was applied to explore the impact of ex vivo expanded Tregs on the neuroinflammation transcriptome.ResultsElimination of mature B and T lymphocytes and natural killer cells in 5xFAD-Rag2KO mice was associated with upregulation of 95 inflammation genes and amplified number of reactive microglia within the dentate gyrus. Administration of ex vivo expanded Tregs reduced amyloid burden and reactive glial cells in the dentate gyrus and frontal cortex of 5xFAD-Rag2KO mice. Interrogation of inflammation gene expression documented down-regulation of pro-inflammatory cytokines (IL1A&B, IL6), complement cascade (C1qa, C1qb, C1qc, C4a/b), toll-like receptors (Tlr3, Tlr4 and Tlr7) and microglial activations markers (CD14, Tyrobp,Trem2) following Treg administration.ConclusionsEx vivo expanded Tregs with amplified immunomodulatory function, suppressed neuroinflammation and alleviated AD pathology in vivo. Our results provide preclinical evidences for Treg cell therapy as a potential treatment strategy in AD.
Grain boundary passivation via balancing feedback of hole barrier modulation in HfO2-x for nanoscale flexible electronics
Authors: Kim Yeon Soo , Chung Harry , Kwon Suhyoun , Kim Jihyun , Jo William 
Published: September 2022

AbstractFlexible electronics has attracted considerable attention owing to its enormous potential for practical applications in various fields. However, the massive strain produced during bending degrades the device. Especially at grain boundaries, due to the accumulation of defects, this degradation is exacerbated in flexible electronic devices. The importance of electrically inactivated grain boundaries increases as devices scale down to the nanoscale. Here, we propose an HfO2-xthin film that can be used as an excellent material for flexible electronics with versatile functionality, especially for grain boundary passivation. Various electrical phases of HfO2-xthin films with conducting to insulating behavior, which originates from oxygen deficiency, have been fabricated on flexible substrates. Furthermore, owing to the most stable charge state of oxygen vacancies, oxygen-deficient HfO2-xshowsp-type conductivity. Current mapping by conductive atomic force microscopy reveals that current flow is hindered at grain boundaries due to the formation of potential barriers. This phenomenon is also observed in bent flexible thin films on convex and concave molds, leading to tensile and compressive strains, respectively. Although the defect concentration increases because of lattice deformation during bending, more holes are trapped at the grain boundaries, resulting in an increased hole barrier height. We believe that grain boundary passivation through hole barrier modulation during bending would pave the way for advances in hafnia-based nanoscale flexible electronics.
Acute and Chronic Effects of Blood Flow Restricted High-Intensity Interval Training: A Systematic Review
Authors: Chua Man Tong , Sim Alexiaa , Burns Stephen Francis 
Published: September 2022

Abstract
Background
The implementation of blood flow restriction (BFR) during exercise is becoming an increasingly useful adjunct method in both athletic and rehabilitative settings. Advantages in pairing BFR with training can be observed in two scenarios: (1) training at lower absolute intensities (e.g. walking) elicits adaptations akin to high-intensity sessions (e.g. running intervals); (2) when performing exercise at moderate to high intensities, higher physiological stimulus may be attained, leading to larger improvements in aerobic, anaerobic, and muscular parameters. The former has been well documented in recent systematic reviews, but consensus on BFR (concomitant or post-exercise) combined with high-intensity interval training (HIIT) protocols is not well established. Therefore, this systematic review evaluates the acute and chronic effects of BFR + HIIT.

Methods
The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used to identify relevant studies. A systematic search on 1 February 2022, was conducted on four key databases: ScienceDirect, PubMed, Scopus and SPORTDiscus. Quality of each individual study was assessed using the Physiotherapy Evidence Database (PEDro) scale. Extraction of data from included studies was conducted using an adapted version of the 'Population, Intervention, Comparison, Outcome' (PICO) framework.

Results
A total of 208 articles were identified, 18 of which met inclusion criteria. Of the 18 BFR + HIIT studies (244 subjects), 1 reported both acute and chronic effects, 5 examined acute responses and 12 investigated chronic effects. Acutely, BFR challenges the metabolic processes (vascular and oxygenation responses) during high-intensity repeated sprint exercise—which accelerates central and peripheral neuromuscular fatigue mechanisms resulting in performance impairments. Analysis of the literature exploring the chronic effects of BFR + HIIT suggests that BFR does provide an additive physiological training stimulus to HIIT protocols, especially for measured aerobic, muscular, and, to some extent, anaerobic parameters.

Conclusion
Presently, it appears that the addition of BFR into HIIT enhances physiological improvements in aerobic, muscular, and, to some extent, anaerobic performance. However due to large variability in permutations of BFR + HIIT methodologies, it is necessary for future research to explore and recommend standardised BFR guidelines for each HIIT exercise type.
Time–frequency co-movement and risk connectedness among cryptocurrencies: new evidence from the higher-order moments before and during the COVID-19 pandemic
Authors: Cui Jinxin , Maghyereh Aktham 
Published: September 2022

AbstractAnalyzing comovements and connectedness is critical for providing significant implications for crypto-portfolio risk management. However, most existing research focuses on the lower-order moment nexus (i.e. the return and volatility interactions). For the first time, this study investigates the higher-order moment comovements and risk connectedness among cryptocurrencies before and during the COVID-19 pandemic in both the time and frequency domains. We combine the realized moment measures and wavelet coherence, and the newly proposed time-varying parameter vector autoregression-based frequency connectedness approach (Chatziantoniou et al. in Integration and risk transmission in the market for crude oil a time-varying parameter frequency connectedness approach. Technical report, University of Pretoria, Department of Economics, 2021) using intraday high-frequency data. The empirical results demonstrate that the comovement of realized volatility between BTC and other cryptocurrencies is stronger than that of the realized skewness, realized kurtosis, and signed jump variation. The comovements among cryptocurrencies are both time-dependent and frequency-dependent. Besides the volatility spillovers, the risk spillovers of high-order moments and jumps are also significant, although their magnitudes vary with moments, making them moment-dependent as well and are lower than volatility connectedness. Frequency connectedness demonstrates that the risk connectedness is mainly transmitted in the short term (1–7 days). Furthermore, the total dynamic connectedness of all realized moments is time-varying and has been significantly affected by the outbreak of the COVID-19 pandemic. Several practical implications are drawn for crypto investors, portfolio managers, regulators, and policymakers in optimizing their investment and risk management tactics.
A case of severe generalized pustular psoriasis successfully treated with IL-17A monoclonal antibody and granulocyte removal therapy
Authors: Shimada Keiki , Katagiri Daisuke , Kato Aika , Nunose Naoto , Sato Motohiko , Katayama Yuri , Terakawa Kanako , Niikura Takahito , Sakamoto Emi , Yoshizaki Yuki , Suzuki Minami , Fukaya Takashi , Tamaki Takeshi , Takano Hideki 
Published: September 2022

Abstract
Background
Generalized pustular psoriasis (GPP) usually presents with fever, generalized flushing, and multiple sterile pustules on the skin, which histopathologically form subcorneal pustules characterized by Kogoj spongiform pustules. Granulocyte/monocyte adsorption apheresis (GMA) was approved in Japan in 2012. The use of biologics for psoriasis treatment is increasing. Several case reports have evaluated the combination of GMA and cyclosporine (CyA) for GPP. However, very few English reports on combining biologics and GMA in treating GPP exist.

Case presentation
A 79-year-old man with a history of hypertension, diabetes mellitus, chronic kidney disease, and atrial fibrillation was admitted. He had been consulting a dermatologist for psoriasis vulgaris (PV) since the age of 44. The patient was diagnosed with severe GPP and treated with 300 mg secukinumab (SEC) on day 3. SEC is a fully human monoclonal IgG1 antibody that targets IL-17A. Five doses were administered. In addition, GMA was administered once a week, three times from day 4. After the first administration of GMA, the inflammatory response and skin condition improved markedly. The patient was discharged from the hospital on day 34.

Conclusions
The present study is the first English-written report on the combined administration of SEC and GMA both instituted since admission for severe GPP, with immediate patient response to treatment. Notably, IL-17A plays a vital role in the pathogenesis of GPP. GMA can eliminate activated leukocytes, and the early introduction of combined IL-17 monoclonal antibody and GMA may allow disease suppression in patients with severe GPP, thus avoiding progression to multiorgan failure. Further studies may verify the effects of IL-17 monoclonal antibodies and GMA on severe GPP.
Effect of entomopathogenic fungi against banana pseudostem weevil Odoiporus longicollis (Olivier) and elucidation of infection process
Authors: Viswakethu Velavan , Ramasamy Vinitha , Krishnamoorthy Saranya , Segar Thilakshana , Ramaraj Aravindh , Kuttithodika Muhammed Aboobacker , Narayanasamy Baskar , Balakrishnan Padmanaban , Rajagopal Rangeshwaran 
Published: September 2022

AbstractBackgroundThe banana stem weevil,Odoiporus longicollis(Olivier), is a serious threat to banana cultivation world over. Since banana is a food crop, the use of naturally infecting biological control agents could be an effective alternative to manage the insect pest instead of harmful chemicals. Also, the efficacy of entomopathogenic fungi againstO. longicolliswas used in bioassay.ResultsAmong theBeauveria bassianaisolates tested the median lethal concentration (LC50) 10.468 × 105conidia ml−1when treated withB. bassiana(NRCBEFPMP1), two other isolates ofB. bassiana, namely NRCBEPF22 and NRCBEPF2, were also effective againstO. longicollisand recorded LC50of 12.617 × 105and 12.891 × 105conidia ml−1, respectively. The results of bioassay with differentMetarhiziumspp. showed variations in efficacy, where the most virulent isolate wasM. quizhouense(NRCBEPF11) with LC508.050 × 105conidia ml−1. Scanning electron microscopic analysis showed thatB. bassianaandM. quizhouensecaused infection by cuticle penetration and completed the infection process in 15 days. The composition of volatile organic compounds released byB. bassianaandM. anisopliaeduring pathogenesis showed that a significantly high number of known insect volatiles were present in infected insects. Consequently, these volatiles were emission in Insect attractant, Odorant receptor agonist, Plant hormone Plant, and Microbial Metabolites, through the biological activity, such as Methyl salicylate, Benzaldehyde, alpha-Terpineol, Limonene, Benzene, 1,2-dimethoxy, Phthalic acid, 1-Octadecene, Phenylacetaldehyde, 3-Octanone, Octanal, Methylheptenone and 2-Ethyl-1-hexyl alcohol.ConclusionOverall, the results show that EPF could significantly reduce damage byO. longicollisand produce a wide profile of secondary metabolites. Further, analysis was used for principal components to determine whether separated classes of fungi can be distinguished from one another based on their metabolite profiles.
Influence of perioperative anesthesia methods or anesthetic agents preferred for gastric cancer surgery on the survival of patients: a narrative review
Authors: Yılmaz Fulya , Bas Koray 
Published: September 2022

Abstract
Background
Gastric cancer is a major global public health problem. It is the fourth most common cancer and the second cause of cancer-related deaths worldwide. Despite advances in the field of medical and radiation oncology, surgical resection is a crucial intervention and remains the mainstay of gold standard treatment. Recently, the effects of anesthesia method(s) and/or anesthetic agent(s) on survival for different types of cancers gained attention. So, we want to summarize the evidences of anesthesia methods and/or anesthetic agents preferred for gastric cancer surgery on the survival.

Main body
The Web of Science software was used for the search and the analysis. To analyze scientific productivity of all scientific papers published about survival of patients due to the anesthesia methods or anesthetic agents on gastric cancer in Science Citation Index Expanded (SCI-E) from 1980 to December 5, 2020, the date of the search was searched by using the terms of “gastric cancer,” “survival,” and “anesthesia” in the topic search section of the software.
As a result, overall, fifteen papers were related to our topic. Four of these studies compared total intravenous anesthesia (TIVA) with general anesthesia, five of these compared general anesthesia with general anesthesia combined with epidural anesthesia/analgesia for gastric cancer, and three of these studies investigated effect of anesthetic agents for gastric cells in in vitro conditions. Other publications were review on this topic.

Conclusions
The important role of anesthesia in treatment of gastric cancer patients is still controversial. Further prospective randomized studies are needed.
Effects of dietary Syzygium aromaticum leaf meal supplementation on blood profile and oxidative status of laying hens
Authors: Adu Olufemi Adesanya , Olateju Imoleayo Sarah , Samuel Adeniyi David , Gbore Francis Ayodeji 
Published: September 2022

Abstract
Background
The study examined the effects of dietary supplementation of Syzygium aromaticum leaf meal (SLM) on the haematology, serum biochemistry and antioxidative status of laying hens.

Results
A total of 150 20-week-old Isa brown birds were randomly assigned to three experimental diets: Diet 1/control (no supplementation), Diet 2 (0.25% SLM) and Diet 3 (0.50% SLM). Each experimental diet was further divided into five (5) replicates and comprised 10 birds per replicate, that is, 50 birds per experimental diet. The birds were fed with layers mash for 32 weeks of the feeding trial. Blood samples collected in the 32nd week were subjected to haematological and serum biochemical analyses. The dietary supplementation of 0.25% and 0.50% SLM did not significantly (P > 0.05) affect the haematological parameters observed in the layer birds which indicated that SLM did not render the birds anaemic nor compromise their immunity. Likewise, the result of the serum protein (total protein, albumin, globulin and albumin: globulin), serum enzymes (alanine and aspartate aminotransferases) and serum metabolites (creatinine, uric acid and glucose) were not affected (P > 0.05) by the dietary treatment except for the concentration of serum catalase and glutathione peroxidase which was statistically higher (P < 0.05) in layers fed with diets supplemented with SLM compared to the control group, while the serum superoxide dismutase concentration of laying hens was not significantly influenced (P > 0.05).

Conclusion
The supplementation of SLM at 0.25% and 0.50% in layers diet can be referred to as a safe additive as the blood indices of birds were not adversely affected. Also, the liver functions which were investigated through the serum enzymes were not impaired and oxidative stress is not triggered in the birds.
Exploring the feasibility of ex-post harmonisation of religiosity items from the European Social Survey and the European Values Study
Authors: Aizpurua Eva , Fitzgerald Rory , de Barros Julia Furtado , Giacomin Gregorio , Lomazzi Vera , Luijkx Ruud , Maineri Angelica , Negoita Daniela 
Published: September 2022

AbstractThis paper examines the feasibility of ex-post harmonisation strategies using European Values Study (EVS) Wave 5 (2017–2020) and European Social Survey (ESS) Round 9 (2018–2019) data across 17 countries. The study shows an empirical assessment of the comparability of four items measuring religious behaviours (belonging to a religious denomination at present/in the past, religious services attendance, and praying), captured in both surveys. The novelty of this paper lies in the analytical comparison of religiosity indicators that are rarely assessed from a comparative perspective.The harmonisation strategy was based upon several analytical techniques that seek to determine similarities and differences between the selected items in terms of (a) their validity, by examining their correlations with a set of sociodemographic and substantive correlates, (b) their distributions, supplemented by visual comparisons and relevant statistical tests, and (c) item non-substantive shares. The findings pointed to the most consistency among the partial correlations, where individual religiosity produced the most differences between the surveys. Distributions produced the most discrepancies that also corresponded to less similarity across variable categories as gauged by Duncan’s index. This paper is descriptive and exploratory in its aim. It can be taken as a jumping-off point for future research where the time series of these two surveys, and potentially others, can be examined across aggregate levels (e.g. birth cohorts, countries).