bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel unconventional T cell population enriched in Crohn’s disease
Authors: Rosati Elisa , Rios Martini Gabriela , Pogorelyy Mikhail V , Minervina Anastasia A , Degenhardt Frauke , Wendorff Mareike , Sari Soner , Mayr Gabriele , Fazio Antonella , Dowds Christel Marie , Hauser Charlotte , Tran Florian , von Schönfels Witigo , Pochhammer Julius , Salnikova Maria A , Jaeckel Charlot , Gigla Johannes Boy , Sabet Sanaz Sedghpour , Hübenthal Matthias , Schiminsky Esther , Schreiber Stefan , Rosenstiel Philip C , Scheffold Alexander , Thomas Paul G , Lieb Wolfgang , Bokemeyer Bernd , Witte Maria , Aden Konrad , Hendricks Alexander , Schafmayer Clemens , Egberts Jan-Hendrick , Mamedov Ilgar Z , Bacher Petra , Franke Andre 
Published: March 2022

ObjectiveOne of the current hypotheses to explain the proinflammatory immune response in IBD is a dysregulated T cell reaction to yet unknown intestinal antigens. As such, it may be possible to identify disease-associated T cell clonotypes by analysing the peripheral and intestinal T-cell receptor (TCR) repertoire of patients with IBD and controls.DesignWe performed bulk TCR repertoire profiling of both the TCR alpha and beta chains using high-throughput sequencing in peripheral blood samples of a total of 244 patients with IBD and healthy controls as well as from matched blood and intestinal tissue of 59 patients with IBD and disease controls. We further characterised specific T cell clonotypes via single-cell RNAseq.ResultsWe identified a group of clonotypes, characterised by semi-invariant TCR alpha chains, to be significantly enriched in the blood of patients with Crohn’s disease (CD) and particularly expanded in the CD8+T cell population. Single-cell RNAseq data showed an innate-like phenotype of these cells, with a comparable gene expression to unconventional T cells such as mucosal associated invariant T and natural killer T (NKT) cells, but with distinct TCRs.ConclusionsWe identified and characterised a subpopulation of unconventional Crohn-associated invariant T (CAIT) cells. Multiple evidence suggests these cells to be part of the NKT type II population. The potential implications of this population for CD or a subset thereof remain to be elucidated, and the immunophenotype and antigen reactivity of CAIT cells need further investigations in future studies.
Role of regional anesthesia and analgesia in enhanced recovery after colorectal surgery: a systematic review of randomized controlled trials
Authors: El-Boghdadly Kariem , Jack James M , Heaney Aine , Black Nick D , Englesakis Marina F , Kehlet Henrik , Chan Vincent W S 
Published: March 2022

BackgroundEffective analgesia is an important element of enhanced recovery after surgery (ERAS), but the clinical impact of regional anesthesia and analgesia for colorectal surgery remains unclear.ObjectiveWe aimed to determine the impact of regional anesthesia following colorectal surgery in the setting of ERAS.Evidence reviewWe performed a systematic review of nine databases up to June 2020, seeking randomized controlled trials comparing regional anesthesia versus control in an ERAS pathway for colorectal surgery. We analyzed the studies with successful ERAS implementation, defined as ERAS protocols with a hospital length of stay of ≤5 days. Data were qualitatively synthesized. Risk of bias was assessed using the Cochrane Risk of Bias 2 tool.FindingsOf the 29 studies reporting ERAS pathways, only 13 comprising 1170 patients were included, with modest methodological quality and poor reporting of adherence to ERAS pathways. Epidural analgesia had limited evidence of outcome benefits in open surgery, while spinal analgesia with intrathecal opioids may potentially be associated with improved outcomes with no impact on length of stay in laparoscopic surgery, though dosing must be further investigated. There was limited evidence for fascial plane blocks or other regional anesthetic techniques.ConclusionsAlthough there was variable methodological quality and reporting of ERAS, we found little evidence demonstrating the clinical benefits of regional anesthetic techniques in the setting of successful ERAS implementation, and future studies must report adherence to ERAS in order for their interventions to be generalizable to modern clinical practice.PROSPERO registration numberCRD42020161200.
Graphite-based sensor amended with fumed silica for electrodetection of azithromycin
Authors: Mamdouh Salma , Shehata M. , Fekry A.M. , Ameer M.A. 
Published: March 2022

Azithromycin (AM) detection has become of great interest as being one of the prescribed medicines in the medication protocol in Egypt for the recent coronavirus disease 2019 pandemic. Herein, a carbon paste electrode was simply amended with fumed silica for determining AM. The characterization of the new material was done by different techniques, including scanning electron microscopy, transmission electron microscopy, and electrochemical impedance spectroscopy. The newly modified fumed silica carbon paste electrode exhibited a highly sensitive response toward the oxidation of 1.0 mmol/L AM in phosphate buffer solution (PBS) for a pH range of 5.0–10.0. The effect of varying AM concentrations was studied in PBS, pH 7.4, with a detection limit of 11 μmol/L and a quantification limit of 37 μmol/L. Eventually, the recently amended electrode attained reasonable sensitivity and constancy for AM detection in actual trials, such as blood plasma and pharmaceutical drugs.
Probing light mediators in the radiative emission of neutrino pair
Authors: Ge Shao-Feng , Pasquini Pedro 
Published: March 2022

AbstractWe propose a new possibility of using the coherently enhanced neutrino pair emission to probe light-mediator interactions between electron and neutrinos. With typical momentum transfer at the atomic $$\mathcal O(1$$

O
(
1

eV) scale, this process is extremely sensitive for the mediator mass range $$\mathcal O(10^{-3} \sim 10^4$$

O
(

10

-
3

∼

10
4

) eV. The sensitivity on the product of couplings with electron ($$g^e$$

g
e

or $$y^e$$

y
e

) and neutrinos ($$g^\nu $$

g
ν

or $$y^\nu $$

y
ν

) can touch down to $$|y^e y^\nu | < 10^{-9} \sim 10^{-19}$$

|

y
e

y
ν

|
<

10

-
9

∼

10

-
19

for a scalar mediator and $$|g^e g^\nu | < 10^{-15} \sim 10^{-26}$$

|

g
e

g
ν

|
<

10

-
15

∼

10

-
26

for a vector one, with orders of improvement from the existing constraints.
Lagrangian cobordisms in Liouville manifolds
Authors: Bosshard Valentin 
Published: March 2022

Floer theory for Lagrangian cobordisms was developed by Biran and Cornea in a series of papers [Lagrangian cobordism. I, J. Amer. Math. Soc. 26 (2013) 295–340; Lagrangian cobordism and Fukaya categories, Geom. Funct. Anal. 24 (2014) 1731–1830; Cone-decompositions of Lagrangian cobordisms in Lefschetz fibrations, Selecta Math. 23 (2017) 2635–2704] to study the triangulated structure of the derived Fukaya category of monotone symplectic manifolds. This paper explains how to use the language of stops to study Lagrangian cobordisms in Liouville manifolds and the associated exact triangles in the derived wrapped Fukaya category. Furthermore, we compute the cobordism groups of non-compact Riemann surfaces of finite type.
Toward Equivalent Transformation of User Preferences in Cross Domain Recommendation
Authors: Chen Xu , Zhang Ya , Tsang Ivor W. , Pan Yuangang , Su Jingchao 
Published: March 2022

Cross domain recommendation (CDR) is one popular research topic in recommender systems. This article focuses on a popular scenario for CDR where different domains share the same set of users but no overlapping items. The majority of recent methods have explored the shared-user representation to transfer knowledge across domains. However, the idea of shared-user representation resorts to learning the overlapped features of user preferences and suppresses the domain-specific features. Other works try to capture the domain-specific features by an MLP mapping but require heuristic human knowledge of choosing samples to train the mapping. In this article, we attempt to learn both features of user preferences in a more principled way. We assume that each user’s preferences in one domain can be expressed by the other one, and these preferences can be mutually converted to each other with the so-called equivalent transformation. Based on this assumption, we propose an equivalent transformation learner (ETL), which models the joint distribution of user behaviors across domains. The equivalent transformation in ETL relaxes the idea of shared-user representation and allows the learned preferences in different domains to preserve the domain-specific features as well as the overlapped features. Extensive experiments on three public benchmarks demonstrate the effectiveness of ETL compared with recent state-of-the-art methods. Codes and data are available online: https://github.com/xuChenSJTU/ETL-master.
Immunostimulatory Cancer-Associated Fibroblast Subpopulations Can Predict Immunotherapy Response in Head and Neck Cancer
Authors: Obradovic Aleksandar , Graves Diana , Korrer Michael , Wang Yu , Roy Sohini , Naveed Abdullah , Xu Yaomin , Luginbuhl Adam , Curry Joseph , Gibson Michael , Idrees Kamran , Hurley Paula , Jiang Peng , Liu X. Shirley , Uppaluri Ravindra , Drake Charles G. , Califano Andrea , Kim Young J. 
Published: March 2022

AbstractPurpose:Cancer-associated fibroblasts (CAF) have been implicated as potential mediators of checkpoint immunotherapy response. However, the extensive heterogeneity of these cells has precluded rigorous understanding of their immunoregulatory role in the tumor microenvironment.Experimental Design:We performed high-dimensional single-cell RNA sequencing (scRNA-seq) on four patient tumors pretreatment and posttreatment from a neoadjuvant trial of patients with advanced-stage head and neck squamous cell carcinoma that were treated with the αPD-1 therapy, nivolumab. The head and neck CAF (HNCAF) protein activity profiles, derived from this cohort of paired scRNA-seq, were used to perform protein activity enrichment analysis on the 28-patient parental cohort of clinically annotated bulk transcriptomic profiles. Ex vivo coculture assays were used to test functional relevance of HNCAF subtypes.Results:Fourteen distinct cell types were identified with the fibroblast population showing significant changes in abundance following nivolumab treatment. Among the fibroblast subtypes, HNCAF-0/3 emerged as predictive of nivolumab response, while HNCAF-1 was associated with immunosuppression. Functionally, HNCAF-0/3 were found to reduce TGFβ-dependent PD-1+TIM-3+ exhaustion of CD8 T cells, increase CD103+NKG2A+ resident memory phenotypes, and enhance the overall cytolytic profile of T cells.Conclusions:Our findings demonstrate the functional importance of distinct HNCAF subsets in modulating the immunoregulatory milieu of human HNSCC. In addition, we have identified clinically actionable HNCAF subtypes that can be used as a biomarker of response and resistance in future clinical trials.
Integrated Genomic Analysis Identifies UBTF Tandem Duplications as a Recurrent Lesion in Pediatric Acute Myeloid Leukemia
Authors: Umeda Masayuki , Ma Jing , Huang Benjamin J. , Hagiwara Kohei , Westover Tamara , Abdelhamed Sherif , Barajas Juan M. , Thomas Melvin E. , Walsh Michael P. , Song Guangchun , Tian Liqing , Liu Yanling , Chen Xiaolong , Kolekar Pandurang , Tran Quang , Foy Scott G. , Maciaszek Jamie L. , Kleist Andrew B. , Leonti Amanda R. , Ju Bengsheng , Easton John , Wu Huiyun , Valentine Virginia , Valentine Marcus B. , Liu Yen-Chun , Ries Rhonda E. , Smith Jenny L. , Parganas Evan , Iacobucci Ilaria , Hiltenbrand Ryan , Miller Jonathan , Myers Jason R. , Rampersaud Evadnie , Rahbarinia Delaram , Rusch Michael , Wu Gang , Inaba Hiroto , Wang Yi-Cheng , Alonzo Todd A. , Downing James R. , Mullighan Charles G. , Pounds Stanley , Babu M. Madan , Zhang Jinghui , Rubnitz Jeffrey E. , Meshinchi Soheil , Ma Xiaotu , Klco Jeffery M. 
Published: March 2022

Abstract

The genetics of relapsed pediatric acute myeloid leukemia (AML) has yet to be comprehensively defined. Here, we present the spectrum of genomic alterations in 136 relapsed pediatric AMLs. We identified recurrent exon 13 tandem duplications (TD) in upstream binding transcription factor (UBTF) in 9% of relapsed AML cases. UBTF-TD AMLs commonly have normal karyotype or trisomy 8 with cooccurring WT1 mutations or FLT3-ITD but not other known oncogenic fusions. These UBTF-TD events are stable during disease progression and are present in the founding clone. In addition, we observed that UBTF-TD AMLs account for approximately 4% of all de novo pediatric AMLs, are less common in adults, and are associated with poor outcomes and MRD positivity. Expression of UBTF-TD in primary hematopoietic cells is sufficient to enhance serial clonogenic activity and to drive a similar transcriptional program to UBTF-TD AMLs. Collectively, these clinical, genomic, and functional data establish UBTF-TD as a new recurrent mutation in AML.

Significance:
We defined the spectrum of mutations in relapsed pediatric AML and identified UBTF-TDs as a new recurrent genetic alteration. These duplications are more common in children and define a group of AMLs with intermediate-risk cytogenetic abnormalities, FLT3-ITD and WT1 alterations, and are associated with poor outcomes.
See related commentary by Hasserjian and Nardi, p. 173.
This article is highlighted in the In This Issue feature, p. 171.
Systemic scleroderma patient education: a booklet report
Authors: Chaves Caio Carvalhais , Marcelino Ana Leticia Fontes de Oliveira , Silva Laura Cristine Carmo da , Andrade Júnia Amorim , Santos Flavia Patrícia Sena Teixeira , Ferreira Gilda Aparecida , Calderaro Débora Cerqueira 
Published: March 2022

Objective: This study aims to describe the development and online distribution of a patient education booklet about systemic scleroderma focused on occupational therapy orientations. Methods: The research-action methodology was applied and divided into the following five steps: analysis of patients' requests, preparation of the content, choice of illustrations and graphic design, developers' approval of the education booklet, and online distribution. Results: The education booklet entitled "Occupational Therapy Guidelines for People with Systemic Sclerosis" was developed, and its online distribution reached nearly 5000 people. Conclusion: Educational materials serve as adjuncts to the treatment of chronic diseases, as systemic scleroderma. The online distribution of these materials expands its reach among these patients.
Treinamento com realidade virtual não imersiva é efetivo na melhora da funcionalidade de idosos institucionalizados e uma opção de atividade física segura nos momentos de restrição: um estudo piloto
Authors: Lopes Diene Gomes Colvara , Myskiw Jociane de Carvalho , Bós Ângelo José Gonçalves , Oliveira Vanice Hohemberge de , Perpétuo Marcelo , Souza Patrícia 
Published: March 2022

O envelhecimento é um processo dinâmico e progressivo que proporciona a diminuição gradual da capacidade funcional, aumentando a dependência dos idosos e culminando, muitas vezes, na sua institucionalização. Neste contexto, a Realidade Virtual Não Imersiva (RVNI) vem se mostrando uma ferramenta segura e efetiva para estimular a funcionalidade em idosos, inclusive diante da situação de distanciamento social que vivemos atualmente. Objetivo: Avaliar, preliminarmente, o efeito do treinamento com jogos de RVNI sobre a funcionalidade de idosos institucionalizados. Método: Estudo intervencional randomizado, com amostra composta por 17 idosos de quaisquer gêneros (idade 87 anos ±5,4 anos), residentes em uma instituição de Porto Alegre, divididos em dois grupos: Grupo Treinamento (GT) e Grupo Controle (GC). Foram avaliadas a escala de independência funcional (MIF) e a circunferência da panturrilha e do braço, bem como foram realizados o teste de Força de Preensão Palmar (FPP) e o Timed Get Up and Go (TUG). O protocolo de intervenção teve duração de 8 semanas, com duas sessões semanais de 30 minutos. Resultados: Os grupos demostraram ser homogêneos em relação ao gênero e idade. O GT apresentou melhora significante em 4 das 5 variáveis analisadas, já o GC apenas no teste de FPP. O GT no FPP apresentou uma diferença estatisticamente significativa de 21,1 ± 12,60 kg para 23,8 ± 13,51 kg (p<0,003). Conclusão: O presente estudo sugere que o treinamento com RVNI é efetivo na melhora da funcionalidade de idosos institucionalizados e pode ser uma opção de intervenção física segura, especialmente em momentos de restrição.
Association Between Temporal Changes in Early Repolarization Pattern With Long‐Term Cardiovascular Outcome: A Population‐Based Cohort Study
Authors: Liu Li‐Juan , Tang Na , Bi Wen‐Tao , Zhang Ming , Deng Xue‐Qiong , Cheng Yun‐Jiu 
Published: March 2022

Background
The prognostic value of early repolarization pattern (ERP) remains controversial. We aim to test the hypothesis that temporal changes in ERP are associated with increased risks for sudden cardiac death (SCD) and cardiovascular death.

Methods and Results
A total of 14 679 middle‐aged participants from the prospective, population‐based cohort were included in this analysis, with ERP status recorded at baseline and during 3 follow‐up visits in the ARIC (Atherosclerosis Risk in Communities) study. We related baseline ERP, time‐varying ERP, and temporal changes in ERP to cardiovascular outcomes. Cox models were used to estimate the hazard ratios (HRs) adjusted for possible confounding factors. With a median follow‐up of 22.5 years, there were 5033 deaths, 1239 cardiovascular deaths, and 571 SCDs. Time‐varying ERP was associated with increased risks of SCD (HR, 1.59 [95% CI, 1.25–2.02]), cardiovascular death (HR, 1.70 [95% CI, 1.44–2.00]), and death from any cause (HR, 1.16 [95% CI, 1.05–1.27]). Baseline ERP was also associated with 3 outcomes. Compared with those with consistently normal ECG findings, subjects with new‐onset ERP or consistent ERP experienced increased risks of developing SCD and cardiovascular death. The time‐varying ERP in women, White subjects, and anterior leads and J‐wave amplitudes ≥0.2 mV appeared to indicate poorer cardiovascular outcomes.

Conclusions
Our findings suggest that baseline ERP, time‐varying ERP, new‐onset ERP, and consistent ERP were independent predictors of SCD and cardiovascular death in the middle‐aged biracial population. Repeated measurements of the ERP might improve its use as a risk indicator for SCD.
Treatment With Icosapent Ethyl to Reduce Ischemic Events in Patients With Prior Percutaneous Coronary Intervention: Insights From REDUCE‐IT PCI
Authors: Peterson Benjamin E. , Bhatt Deepak L. , Steg Ph. Gabriel , Miller Michael , Brinton Eliot A. , Jacobson Terry A. , Ketchum Steven B. , Juliano Rebecca A. , Jiao Lixia , Doyle Ralph T. , Granowitz Craig , Gibson C. Michael , Pinto Duane , Giugliano Robert P. , Budoff Matthew J. , Tardif Jean‐Claude , Verma Subodh , Ballantyne Christie M. ,   the REDUCE‐IT Investigators
Published: March 2022

Background
Patients who undergo percutaneous coronary intervention (PCI) are at increased risk for recurrent cardiovascular events despite aggressive medical therapy.

Methods and Results

This post hoc analysis focused on the subset of patients with prior PCI enrolled in REDUCE‐IT (Reduction of Cardiovascular Events With Icosapent Ethyl–Intervention Trial), a multicenter, randomized, double‐blind, placebo‐controlled trial of icosapent ethyl versus placebo. Icosapent ethyl was added to statins in patients with low‐density lipoprotein cholesterol <100 mg/dL and fasting triglycerides 135–499 mg/dL. The primary end point was a composite of cardiovascular death, nonfatal myocardial infarction, nonfatal stroke, coronary revascularization, or unstable angina requiring hospitalization. There were 8179 patients randomized in REDUCE‐IT followed for a median of 4.9 years, and 3408 (41.7%) of them had a prior PCI with a median follow‐up of 4.8 years. These patients were randomized a median of 2.9 years (11 days to 30.7 years) after PCI. Among patients treated with icosapent ethyl versus placebo, there was a 34% reduction in the primary composite end point (hazard ratio [HR], 0.66; 95% CI, 0.58–0.76;
P
<0.001; number needed to treat
4.8 years
=12) and a 34% reduction in the key secondary composite end point of cardiovascular death, nonfatal myocardial infarction, or nonfatal stroke (HR, 0.66; 95% CI, 0.56–0.79;
P
<0.001; NNT
4.8 years
=19) versus placebo. Similarly, large reductions occurred in total coronary revascularizations and revascularization subtypes. There was also a 39% reduction in total events (rate ratio, 0.61; 95% CI, 0.52–0.72;
P
<0.001).

Conclusions
Among patients treated with statins with elevated triglycerides and a history of prior PCI, icosapent ethyl substantially reduced the risk of recurrent events during an average of ~5 years of follow‐up with a number needed to treat of only 12.

Registration

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT01492361.
Impact of Percutaneous Transluminal Renal Angioplasty on Autonomic Nervous System and Natriuresis in Hypertensive Patients With Renal Artery Stenosis
Authors: Iwashima Yoshio , Kusunoki Hiroshi , Taniyama Akira , Horio Takeshi , Hayashi Shin‐ichiro , Kishida Masatsugu , Fukuda Tetsuya , Yoshihara Fumiki , Ishimitsu Toshihiko , Kawano Yuhei 
Published: March 2022

Background
We investigated the early postoperative effect of percutaneous transluminal renal angioplasty on ambulatory blood pressure (BP) and the circadian characteristics of natriuresis and autonomic nerve activity.

Methods and Results

A total of 64 patients with hypertension with hemodynamically significant renal artery stenosis (mean age, 60.0±21.0 years; 31.3% fibromuscular dysplasia) who underwent angioplasty were included, and circadian characteristics of natriuresis as well as heart rate variability indices, including 24‐hour BP, low‐frequency and high‐frequency (HF) components, and the percentage of differences between adjacent normal R‐R intervals >50 ms were evaluated using an oscillometric device, TM‐2425, both at baseline and 3 days after angioplasty. In both the fibromuscular dysplasia and atherosclerotic stenosis groups, 24‐hour systolic BP (fibromuscular dysplasia, −19±14; atherosclerotic renal artery stenosis, −11±9 mm Hg), percentage of differences between adjacent normal R‐R intervals >50 ms, HF, brain natriuretic peptide, and nighttime urinary sodium excretion decreased (all
P
<0.01), and heart rate increased (both
P
<0.05) after angioplasty. In both groups, revascularization increased the night/day ratios of percentage of differences between adjacent normal R‐R intervals >50 ms (both
P
<0.01) and HF, and decreased those of low frequency/HF (all
P
<0.05) and nighttime urinary sodium excretion (fibromuscular dysplasia, 1.17±0.15 to 0.78±0.09; atherosclerotic renal artery stenosis, 1.37±0.10 to 0.99±0.06, both
P
<0.01). Multiple logistic regression analysis indicated that a 1‐SD increase in baseline low frequency/HF was associated with at least a 15% decrease in 24‐hour systolic BP after angioplasty (odds ratio, 2.30 [95% CI, 1.03–5.67];
P
<0.05).

Conclusions
Successful revascularization results in a significant BP decrease in the early postoperative period. Intrarenal perfusion might be a key modulator of the circadian patterns of autonomic nerve activity and natriuresis, and pretreatment heart rate variability evaluation seems to be important for treatment success.