bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue expression of the antiapoptotic protein survinin as a potential biomarker of prostate cancer
Authors: Aboyan I. A. , Fedotova E. N. , Lemeshko S. I. , Maksimov A. Yu. , Komarova E. F. 
Published: August 2022

Background. Excessive expression of survivin is associated with inhibition of cell death, activated by extrinsic or intrinsic apoptotic pathways. The survininin overexpression has been shown in various malignancies, including lung cancer, pancreatic and breast cancer, colon cancer, oral squamous cell carcinoma and high grade non-Hodgkin lymphomas.Aim. To investigate the level of survivin expression in prostate cancer tissues, and evaluate it as a diagnostic marker of prostate cancer.Materials and methods. The level of survivin expression and its subcellular localization were assessed immunohistochemically in patients with prostate cancer (n = 64) and benign prostatic hyperplasia (n = 33). Tissue samples obtained at transrectal biopsy were used for analysis. Prostate cancer samples obtained after cystprostatectomy in patients with normal prostate specific antigen level and normal ultrasound findings (n = 36) were considered control tissue (norm).Results. In prostate cancer group 3+ samples with a high level of survivin expression were present in 48.4 % of cases. In benign prostatic hyperplasia group the majority of samples were assessed as 2+, while 9.1 % of samples were negatively stained. 100 % of normal epithelium samples were negative. In patients with Gleason score <7 a survivin expression level was less than 3+ in 62.5 % of cases, and in patients with Gleason score >7 a highly positive reaction was detected in 68.8 % of cases. A high level of survivin expression was found in the large proportion of tissue samples at prostate specific antigen levels >10 ng/ml. Almost 50 % of highly positive cells were detected at a prostate health index (PHI) value of ≥60. The largest percentage of negative staining for surviving was common with PHI value <25. The degrees of staining for survining 1+ and 2+ prevailed in patients with prostate health index density (DPHI) <0.8, while a high level of prostate cells staining 3+ was observed at >0.8. As a Gleason score increase we observe the change of staining type for nucleocytoplasmic, and the largest number of samples has a staining degree of 2+ at a score GG4–5 (≥4 + 4). The type and frequency of prostate tissue samples staining were not differ depending on the initial prostate specific antigen level.Conclusion. Immunohistochemical assessment of the survivin level, including its subcellular localization, could be considered as tumor-associated and a potential biomarker for differential diagnosis and prediction of prostate cancer course.
Environmental assessment of rainwater harvesting and solar system for agriculture
Authors: Korkmaz Murat 
Published: August 2022

As a result of increasing industrialisation and the use of machines, the demand for both energy and water are increasing. As a result, it is necessary to develop sustainable environmental structural systems in both energy production and irrigation water. This, in turn, requires the most accurate use of existing water resources and the immediate implementation of sustainable agricultural systems. Despite this, none of the agricultural enterprises are yet using systems such as rainwater harvesting and solar power techniques. The main aim of this research is to find effective results in terms of savings and to contribute to the prevention of water scarcity in the future. At the same time, the advantages of environmentally friendly zero-energy roofs and rainwater collection systems are evaluated. Elazig province of Turkey was selected for this study. When the benefits of the solar power plant to be built on the relevant farm and an average tree are compared in terms of reducing the CO2 emissions, it was found that the contribution of this plant would be equal to that of planting 113,506 trees.

Keywords: CO2, emissions, energy, rainwater, renewable energy, solar;
Tindak Tutur Direktif Dalam Surah Az-Zumar (Studi Analisis Pragmatis)
Authors: Kamala Sulis Alfaini , Rohmad Rohmad 
Published: August 2022

This study aims to explain the two forms and functions of directive speech acts in Surah az-zumar. The selection of verses in Surah Az-Zumar is used as the object of study because in the Surah there are verses that contain directive speech. This study uses a qualitative descriptive analysis method with the main source of research being the verses in Surah Az-Zumar. The data collection uses the critical reading method and the data analysis technique uses a content analysis approach. The results of the analysis state that the form of directive speech acts in Surah Az-Zumar includes direct speech acts found 8 utterances and indirect speech acts found 3 utterances. Meanwhile, the function of the directive speech act in Surah Az-Zumar is as follows: the directive speech act of command has 6 utterances, the speech act of the advice directive has 1 utterance, the speech act of the prohibition directive has 1 utterance, the speech act of invitation has 1 utterance, the directive speech act of request has 1 utterance , and the directive speech act of criticism has 1 utterance. With this research, it can add insight and understanding of the forms of directive speech acts in the Qur'an and understand the functions of directive speech acts in the Qur'an.

Abstrak
Penelitian ini mempunyai tujuan untuk menjelaskan tentang 2 bentuk dan fungsi tindak tutur direktif dalam surah az-zumar. Pemilihan ayat-ayat dalam surah az-zumar dijadikan sebagai obyek kajian karena dalam surah tersebut terdapat ayat-ayat yang mengandung tuturan direktif. penelitian ini menggunakan metode analisis deskriptif kualitatif dengan sumber utama penelitian adalah ayat-ayat dalam surah az-zumar. Pengumpulan data menggunakan metode critical reading dan teknik analisis data menggunakan pendekatan analisis isi. Hasil analisis menyatakan bahwa bentuk tindak tutur direktif dalam surah az-zumar mencakup tindak tutur langsung ditemukan 8 tuturan dan tindak tutur tidak langsung ditemukan 3 tuturan. Sedangkan fungsi tindak tutur direktif dalam surah az-zumar sebagai berikut: tindak tutur direktif perintah ada 6 tuturan, tindak tutur direktif nasehat ada1 tuturan , tindak tutur direktif larangan ada 1 tuturan, tindak tutur ajakan ada 1 tuturan, tindak tutur direktif permintaan ada 1 tuturan, dan tindak tutur direktif kritikan ada 1 tuturan. Dengan adanya penelitian ini dapat menambah wawasan serta pemahaman tentang bentuk tindak tutur direktif dalam al-Qur’an serta memahami fungsi-fungsi tindak tutur direktif dalam al-Qur’an.
Porous Liquids Responsive to Light
Authors: Dinker Manish Kumar , Zhao Kan , Dai Zhengxing , Ding Lifeng , Liu Xiao-Qin , Sun Lin-Bing 
Published: August 2022

Abstract
Porous liquid is a unique liquid medium that combines permanent porosity of porous solid with the fluidity of liquid, and this special characteristic makes it potential in various applications. Here we report the first photoresponsive porous ionic liquid (PPIL) by solubilizing photoresponsive metal-organic polyhedron (PMOP, constructed from dicopper and azobenzene-containing carboxylate) in polyethylene-glycol-functionalized bulky ionic liquid (IL). Owing to favorable ion interactions, bulky IL molecules encircle outside PMOP cages, and the inter cavities are maintained. The azobenzene moieties can be isomerized freely in the obtained PPILs to expose and shelter active sites upon visible and UV light irradiations. Hence, the adsorption capacity of PPILs is controllable by light irradiation, and the change in CO2 uptake is up to 30% which is different from neat IL with negligible change. This study might inspire the development of new adsorption process regulated by light instead of conventional pressure and temperature swing adsorption technologies.
Giant Enhancement of Second-Harmonic Generation in Hybrid Metasurface Coupled MoS2 with Fano-Resonance Effect
Authors: Xie Yunfei , Yang Liuli , Du Juan , Li Ziwei 
Published: August 2022

Abstract
Plasmonic nanostructures have been regarded as potential candidates for boosting the nonlinear up-conversion rate at the nanoscale level due to their strong near-field enhancement and inherent high design freedom. Here, we design a hybrid metasurface to realize the moderate interaction of Fano resonance and create the dual-resonant mode-matching condition to facilitate the nonlinear process of second harmonic generation (SHG). The hybrid metasurface presents dipolar and octupolar plasmonic modes near the fundamental and doubled-frequency wavelengths, respectively, further utilized to enhance the SHG of low-dimensional MoS2 semiconductors. The maximum intensity of SHG in hybrid metasurface coupled MoS2 is more than ten thousand times larger than that of other structure-units coupled MoS2. The conversion efficiency is reported to be as high as 3.27×10− 7. This work paves the way to optimize nonlinear light-matter interactions in low-dimensional structures coupled with semiconductors.
Protective Masks During the SARS-COV-2 Pandemic: Is there a Relationship with Temporomandibular Disorders? An Observational Cross-sectional Survey
Authors: d’Apuzzo Fabrizia , Rotolo Rossana Patricia , Nucci Ludovica , Simeon Vittorio , Minervini Giuseppe , Perillo Letizia , Grassia Vincenzo 
Published: August 2022

Abstract
Background
Wearing protective face masks has been one of the indispensable measures to prevent droplets and aerosol particles transmission during the SARS-CoV-2 pandemic. This observational cross-sectional survey investigated the different types and modalities of protective mask wearing and the possible association with referred temporomandibular disorders by respondents.
Methods
An online-questionnaire was developed, calibrated and administered anonymously to subjects with an age of ≥ 18 years. It consisted of different sections: demographics, type and wearing modalities of the protective masks, pain in the preauricular area, noise at the temporomandibular joints and headache. Statistical analysis was performed with R software.
Results
The questionnaire received 665 replies mainly from participants aged 18–30 years (315 males, 350 females). 37% of participants were healthcare professionals, 21.2% of them were dentists. 334 subjects (50.3%) mostly used the Filtering Facepiece 2/ Filtering Facepiece 3 (FFP2/FFP3) mask, and 578 (87%) wore the mask with two elastics behind the ears. 400 participants referred pain while wearing the mask, and 36.8% of them referred pain with a consecutive use of more than 4 hours (p = 0.042). 92.2% of participants did not report any TMJ noise. 57.7% of subjects referred headaches associated with the FFP2/FFP3 (p = 0.033).
Conclusions
This survey highlighted the increased referred presence of orofacial pain in the preauricular area and temporal headache associated with a prolonged use of protective face masks for more than 4 hours during the SARS-CoV-2 pandemic.
Clinical characteristics of nasolacrimal duct obstruction after iodine therapy of DTC patients
Authors: Li Na , Zhang Wanchun 
Published: August 2022

Abstract
Background: The aim was to analyze clinical characteristics of differentiated thyroid carcinoma (DTC) patients with nasolacrimal duct obstruction after 131I therapy and provide guidance for clinical decision-making. Methods: Thirty one DTC patients with nasolacrimal duct obstruction were retrospectively enrolled from the Nuclear Medicine Department of Shanxi Bethune Hospital during follow-up of 131I therapy between June 2018 and March 2021.Sixty one thyroid cancer patients without nasolacrimal duct obstruction after 131I therapy were also enrolled to act as a control group. Clinical characteristics, including sex, age, dose, positive anti-thyroglobulin antibodies (TGAb), metastasis and nasal uptake, were analyzed by χ2 test and logistic multifactor regression. Results: Statistically significant differences were found in patients’ nasal uptake after 131I therapy according to gender (11.8% : 38.7%), age (5.6% : 51.1%), dose (3.4% : 47.6%), stage (18.5% : 70.4%) and iodine uptake (83.3% : 30.2%). There was no significant difference in the proportion of TGAb positive and negative patients according to 131I therapy (χ2=0.27, P=0.782). Multivariate logistic regression analysis showed that gender (2.59), age (1.45), dose (2.36), whether there was iodine uptake of metastatic foci (1.93) and whether there was nasal uptake of iodine (1.70) were all statistically significant influential factors of lacrimal discharge symptoms after iodine therapy. Conclusion: Patients who were over 50, women, had metastatic iodine uptake, received a dose > 5.55GBq and had nasal intake of 131I were more likely to have nasolacrimal duct obstruction. When determining therapeutic doses of 131I, doctors should weigh multiple factors and suggest that high risk populations be referred to the appropriate ophthalmic surgery for timely diagnosis and therapy.
PrEP knowledge and HIV testing preferences among street-based female sex workers in the United States: Implications post-COVID-19
Authors: Bouey Jennifer Z.H. , Leonard Madeline , Les Elaine , Hofley Carolyn , Gribble Rebecca , Garip Nabila Mella , Sternberg David 
Published: August 2022

Abstract
Female sex workers face a disproportionately high burden of HIV infection globally, yet their knowledge and attitudes towards new HIV prevention methods are understudied in the United States. We conducted focus group discussions and in-depth interviews in a U.S. city among street-based cis- and trans- female sex workers to identify the knowledge, attitudes, and behaviors regarding PrEP and HIV testing in collaboration with a harm-reduction community service organization. We found study participants had limited knowledge of PrEP and poor communication between female sex workers and primary care physicians a barrier to HIV prevention. Participants also expressed strong preferences for HIV testing to be conducted by medical professionals compared to self-testing. Improvement in communication between urban low-income at-risk women and their primary care physicians, and culturally sensitive HIV testing strategies, are critical to the post-COVID-19 strategy on recovery of community services targeting the most vulnerable urban populations.
Kaempferide exhibits an anticancer effect via caspase dependant apoptosis against Hepatocellular carcinoma in vitro and in vivo
Authors: Chandrababu Gopika , Varkey Merlin , Devan Aswathy R , V Anjaly M , Nath Lekshmi R 
Published: August 2022

Abstract
Context:
Phytochemicals have been promising candidates for cancer therapy, affecting various cancer initiation and progression stages. Kaempferide is a mono methoxy flavone that shows potent anticancer effects on multiple cancers both in vitro and in-vivo.
Materials and Methods
We evaluated the anticancer activity of Kaempferide against HCC using MTT assay. HepG2, Huh7 and N1S1 were used for preliminary in vitro studies. This is followed by an apoptosis analysis as assessed by Caspase 3 and Caspase 9 and Cell cycle analysis. The in-vivo effects of the compound were studied in N1S1 orthotopically injected SD rat model, where the animal was given Kaempferide (25mg/kg thrice a week) and Vehicle (Cremophor: Ethanol). The expression of caspase 9 and a critical tumor marker, TGF-β were assessed in both control and treatment tumor samples.
Results
Kaempferide-induced dose-dependent cytotoxicity in 3 HCC cell lines (HepG2 IC50 = 25µM, Huh7 IC50 = 18µM and N1S1 IC50 = 20 µM). Further, caspase-dependent apoptosis was also confirmed in vitro. Kaempferide exhibited S phase arrest in HepG2 cells as analysed by Cell cycle analysis. Kaempferide showed a significant reduction in tumor size and tumor volume in vivo. Histopathological evaluation by H &E staining confirmed that altered cells were significantly destroyed in the Kaempferide-treated animals, which correlates with tumor reduction compared to the vehicle-treated group. Caspase 9 levels were also found to be increased in the treatment group. TGF- β1, a crucial marker found in HCC that plays a major role in migration and proliferation of cells, was also downregulated in the treatment group (Control = 207.8 ± 22.9 pg/ml and Kaempferide-treated = 157.3 ± 13.8 pg/ml).
Conclusion
This study confirmed the potential of Kaempferide as a promising alternative against HCC.
Bilateral Psoas Haematoma Following Extracorporeal Membranous Oxygenation in a Patient with Severe COVID-19 (SARS CoV-2). A Rare Case Report and Literature Review.
Authors: Gill Simran , Fragkakis Evangelos Minas , Papworth Megan , Vasilikos Ioannis , Chiarini Stella , Bernard Jason , Bishop Timothy , Lui Darren F. 
Published: August 2022

Abstract
IntroductionAs the COVID-19 pandemic evolves periodically, becomes evident that although affects primarily the respiratory system, its ability to trigger a significant inflammatory response, combined with neuropathy and coagulopathy, often evolves into complex systemic responses as opposed to a purely respiratory disease. This can lead into severe respiratory distress and failure, septic shock, arrhythmia and a multiple organ dysfunction; Circumstances where first-line non-invasive medical therapies become suboptimal and advanced lifesaving intervention including, but not limited to induced coma, intubation and veno-venous extracorporeal membranous oxygenation (VV-ECMO) might be required.Case PresentationAuthors present a rare case of a 58 year old male with a rapid deterioration following COVID-19 infection, requiring immediate intubation at the Emergency Department, which escalated with transfer to a VV-ECMO unit due to unresponsiveness on all standard medical therapies. During the induced coma under VV-ECMO, a bilateral psoas haematoma (PH) insidiously developed, with subsequent lumbar plexopathy complicated by a COVID-19-related mononeuritis multiplex, diagnosed only at a later stage during rehabilitation. Early haematoma evacuation was deemed unsafe, because of his frailty. To the best of our knowledge, this is the first description of a complex bilateral PH, requiring 198 days of in-hospital stay, combined efforts of 3 Teaching hospitals and an ad-hoc multidisciplinary team, for its management.ConclusionsAs the pandemic persists more patients might require VV-ECMO, which however is not risk-free mainly due to the fine balance between COVID-19 coagulopathy and VV-ECMO anti-coagulation protocols. Authors hope to increase awareness around complications and clinical challenges, providing reflections on their early recognition and management.
Single-Atom Ru-zeolite isomerization catalysis for a bio-based route towards terephthalates from muconic acid
Authors: Khalil Ibrahim , Rigamonti Marco Giulio , Janssens Kwinten , Bugaev Aram , Donckels Thibaut , Robijns Sven , De Vos Dirk , Dusselier Michiel 
Published: August 2022

Abstract

The search for sustainable polymers requires new ways for accessing monomers from biomass as raw materials. Cis,cis-muconic acid (ccMA), derived from glucose, stands as a potential intermediate to produce valuable monomers, including terephthalic and adipic acids. However, to access terephthalic acid through Diels-Alder cycloaddition, an additional isomerization of ccMA into ttMA (trans,trans-isomer) is required. In this work, we develop a single-atom low-loaded Ru catalyst (0.2 wt.%), supported on *BEA zeolite, that produces tt-muconate in ethanol with total conversion (to equilibrium) and selectivity >95%. We reached productivity rates of 427 mM.h-1 (≈85 g.L-1.h-1), surpassing, by an order of magnitude, the highest values reported for biobased production of 17 mM.h-1 of ccMA, thus making the latter the rate-determining step if both processes were linked. Furthermore, we demonstrate the direct production of terephthalate intermediates with high yields (78%) and selectivity (80%) in a one-pot isomerization/Diels-Alder reaction. This single atom Ru-zeolite isomerizes dienes via a hydride mechanism and could serve the isomerization of other value-added alkenes.
Hybrid AI-based iBeacon Indoor Positioning Cybersecurity Attacks and Defenses Thereof
Authors: Huang Chi-Jan , Chi Cheng-Jan , Hung Wei-Tzu 
Published: August 2022

Abstract
Currently, iBeacon systems have been increasingly established in public areas to position people and assist users in indoor for location navigation. People receive the services through the Bluetooth Low Energy (BLE) installed on their mobile phones. However, the positioning and navigation functions of a iBeacon system may be compromised when faced with cyberattacks issued by hackers. In other words, its security needs to be further considered and enhanced. This study takes the iBeacon system built in Taipei Main Station, the major transportation hub with daily traffic of at least 300 thousand passengers, as an example for exploring its potential attacks and further studying on the defense technologies under the assistance of AI techniques and human participation. Our experiments demonstrate that the prior information security planning of a iBeacon system and the rolling coding encryption on its issued messages in Taipei Main Station, are the best defense methods.
The oncogenic roles of John Cunningham virus T antigen in digestive epithelial cells with tissue specificity
Authors: Zheng Huachuan , Xue Hang , E Ying , Cui Zhengguo 
Published: August 2022

Abstract
Background
John Cunningham virus (JCV), a ubiquitous polyoma virus that commonly infects the human, is identified as the etiologic factor for progressive multifocal leukoencephalopathy and cancers.
Methods
Here, the transgenic mice of CAG-loxp-Laz-loxp T antigen were established and T antigen expression was especially activated in gastroenterological target cells with LacZ deletion using cre-loxp system.
Results
Gastric poorly-differentiated carcinoma was observed in T antigen-activated mice using K19-cre (stem-like cells) and PGC-cre (chief cells), but not Atp-4b-cre (parietal cells) or Capn8-cre (pit cells) mice. There appeared spontaneous hepatocellular and colorectal cancers in Alb-cre (hepatocytes)/T antigen and villin-cre (intestinal cells)/T antigen transgenic mice. Gastric, colorectal and breast cancer was observed in PGC-cre/T antigen mice. Pancreatic insulinoma and ductal adenocarcinoma, gastric adenoma, and duodenal cancer were detected in Pdx1-cre/T antigen mice. There was alternative splicing of T antigen mRNA in all target organs of these transgenic mice.
Conclusions
It was suggested that JCV T antigen might induce gastroenterological carcinogenesis at a manner of cell specificity. These spontaneous tumor models provide good tools to investigate the oncogenic role of T antigen in digestive cancers.