bluuebunny/crossref_metadata_2025_split huggingface dataset:
An electronic biosensor based on semiconducting tetrazine polymer immobilizing matrix coated on rGO for carcinoembryonic antigen
Authors: Joshi Sowmya , Raj K. Aswani , Rao M. Rajeswara , Ghosh Ruma 
Published: February 2022

AbstractPoint-of-care devices are expected to play very critical roles in early diagnosis and better treatment of cancer. Here, we report the end-to-end development of novel and portable biosensors for detecting carcinoembryonic antigen (CEA), a cancer biomarker, almost instantly at room temperature. The device uses reduced graphene oxide (rGO) as the base conducting layer and a novel poly[(1,4-phenylene)-alt-(3,6-(1,2,4,5-tetrazine)/3,6-(1,2,4,5-dihydrotetrazine))] (PhPTz) as an immobilizing matrix for the CEA antibodies. Judiciously introduced nitrogen-rich semiconducting PhPTz brings multiple advantages to the device—(1) efficiently immobilizes anti-CEA via synergistic H-bonding with peptide and N-glycal units and (2) transports the charge density variations, originated upon antibody-antigen interactions, to the rGO layer. The CEA was dropped onto the anti-CEA/PhPTz/rGO devices at ambient conditions, to facilitate binding and the change in current flowing through the sensors was measured. A response of 2.75–33.7 μA was observed when the devices were tested for a broad range of concentrations (0.25 pg/mL to 800 ng/mL) of CEA. A portable read-out circuit was assembled using Arduino UNO and a voltage divider circuit, and a simple algorithm was developed for the classification of the CEA concentrations. The prediction accuracy of the interfacing electronics along with the algorithm was found to be 100%.
National water shortage for low to high environmental flow protection
Authors: Vanham Davy , Alfieri Lorenzo , Feyen Luc 
Published: February 2022

AbstractGlobal freshwater biodiversity has been decreasing rapidly, requiring the restoration and maintenance of environmental flows (EFs) in streams and rivers. EFs provide many ecosystem services that benefit humans. Reserving such EFs for aquatic ecosystems, implies less renewable water availability for direct human water use such as agriculture, industry, cities and energy. Here we show that, depending on the level of EF protection, global annual renewable water availability for humans decreases between 41 and 80% compared to when not reserving EFs. With low EF protection, currently 53 countries experience different levels of water shortage, which increases to 101 countries for high EF protection. Countries will carefully have to balance the amount of water allocated to humans and the environment.
A sustainable natural clam shell derived photocatalyst for the effective adsorption and photodegradation of organic dyes
Authors: Qu Ting , Yao Xinxin , Owens Gary , Gao Liangjun , Zhang Hailong 
Published: February 2022

AbstractIn response to an increasing desire for modern industries to be both green and sustainable, there has been increasing research focus on the reutilization of natural waste materials to effectively remove and degrade toxic wastewater effluents. One interesting food industry waste product is clam shell. Here a new photocatalytic nanomaterial derived from marine clam shells was successfully prepared and characterized. Thereafter the material was applied for the removal of two target dyes from aqueous solution, where the effect of both catalyst dose and initial dye concentration on adsorption and photocatalysis was investigated. The maximum adsorption capacities of methylene blue (100 mg/L) and Congo red (500 mg/L) were 123.45 mg/g and 679.91 mg/g, respectively, where adsorption followed pseudo second order kinetics predominantly via a chemical adsorption process. The photodegradation removal efficiencies of the two dye solutions under visible light irradiation were 99.6% and 83.3% for MB and CR, respectively. The excellent degradation performance in a mixed dye solution, with strong degradation capability and low cost, demonstrated that the clam shell catalyst material was a good candidate for practical field remediation of dye contaminated wastewater.
Evaluation of an 131I-labeled HER2-specific single domain antibody fragment for the radiopharmaceutical therapy of HER2-expressing cancers
Authors: Feng Yutian , Meshaw Rebecca , McDougald Darryl , Zhou Zhengyuan , Zhao Xiao-Guang , Jannetti Stephen A. , Reiman Robert E. , Pippen Erica , Marjoram Robin , Schaal Jeffrey L. , Vaidyanathan Ganesan , Zalutsky Michael R. 
Published: February 2022

AbstractRadiopharmaceutical therapy (RPT) is an attractive strategy for treatment of disseminated cancers including those overexpressing the HER2 receptor including breast, ovarian and gastroesophageal carcinomas. Single-domain antibody fragments (sdAbs) exemplified by the HER2-targeted VHH_1028 evaluated herein are attractive for RPT because they rapidly accumulate in tumor and clear faster from normal tissues than intact antibodies. In this study, VHH_1028 was labeled using the residualizing prosthetic agent N-succinimidyl 3-guanidinomethyl 5-[131I]iodobenzoate (iso-[131I]SGMIB) and its tissue distribution evaluated in the HER2-expressing SKOV-3 ovarian and BT474 breast carcinoma xenograft models. In head-to-head comparisons to [131I]SGMIB-2Rs15d, a HER2-targeted radiopharmaceutical currently under clinical investigation, iso-[131I]SGMIB-VHH_1028 exhibited significantly higher tumor uptake and significantly lower kidney accumulation. The results demonstrated 2.9 and 6.3 times more favorable tumor-to-kidney radiation dose ratios in the SKOV-3 and BT474 xenograft models, respectively. Iso-[131I]SGMIB-VHH_1028 was prepared using a solid-phase extraction method for purification of the prosthetic agent intermediate Boc2-iso-[131I]SGMIB that reproducibly scaled to therapeutic-level doses and obviated the need for its HPLC purification. Single-dose (SKOV-3) and multiple-dose (BT474) treatment regimens demonstrated that iso-[131I]SGMIB-VHH_1028 was well tolerated and provided significant tumor growth delay and survival prolongation. This study suggests that iso-[131I]SGMIB-VHH_1028 is a promising candidate for RPT of HER2-expressing cancers and further development is warranted.
Genome-wide diversity analysis to infer population structure and linkage disequilibrium among Colombian coconut germplasm
Authors: Muñoz-Pérez Jorge Mario , Cañas Gloria Patricia , López Lorena , Arias Tatiana 
Published: February 2022

AbstractGenetic diversity and relatedness of accessions for coconut growing in Colombia was unknown until this study. Here we develop single nucleotide polymorphisms (SNPs) along the coconut genome based on Genotyping by Sequencing (GBS) with the goal of analyze the genetic diversity, population structure, and linkage disequilibrium (LD) of a diverse coconut panel consisting of 112 coconut accessions from the Atlantic and Pacific coasts of Colombia. A comprehensive catalog of approximately 40,000 SNPs with a minor allele frequency (MAF) of > 0.05 is presented. A total of 40,614 SNPs were found but only 19,414 anchored to chromosomes. Of these, 10,338 and 4606 were exclusive to the Atlantic and Pacific gene pools, respectively, and 3432 SNPs could differentiate both gene pools. A filtered subset of unlinked and anchored SNPs (1271) showed a population structure at K = 4, separating accessions from the Pacific and Atlantic coasts that can also be distinguished by palm height, as found in previous studies. The Pacific groups had a slow LD decay, low Fixation Index (Fst) and low nucleotide diversity (π), while the Atlantic group had slightly higher genetic diversity and faster LD decay. Genome-wide diversity analyses are of importance to promote germplasm conservation and breeding programs aimed at developing new cultivars better adapted to the region.
The role of the endolithic alga Ostreobium spp. during coral bleaching recovery
Authors: Galindo-Martínez Claudia Tatiana , Weber Michele , Avila-Magaña Viridiana , Enríquez Susana , Kitano Hiroaki , Medina Mónica , Iglesias-Prieto Roberto 
Published: February 2022

AbstractIn this study, we explore how the Caribbean coral Orbicella faveolata recovers after bleaching, using fragments from 13 coral colonies exposed to heat stress (32 °C) for ten days. Biological parameters and coral optical properties were monitored during and after the stress. Increases in both, the excitation pressure over photosystem II (Qm) and pigment specific absorption (a*Chla) were observed in the stressed corals, associated with reductions in light absorption at the chlorophyll a red peak (De675) and symbiont population density. All coral fragments exposed to heat stress bleached but a fraction of the stressed corals recovered after removing the stress, as indicated by the reductions in Qm and increases in De675 and the symbiont population observed. This subsample of the experimentally bleached corals also showed blooms of the endolithic algae Ostreobium spp. underneath the tissue. Using a numerical model, we quantified the amount of incident light reflected by the coral, and absorbed by the different pigmented components: symbionts, host-tissue and Ostreobiumspp. Our study supports the key contribution of Ostreobiumspp. blooms near the skeletal surface, to coral recovery after bleaching by reducing skeleton reflectance. Endolithic blooms can thus significantly alleviate the high light stress that affects the remaining symbionts during the stress or when the coral has achieved the bleached phenotype.
Effects of pulse chirp on laser-driven proton acceleration
Authors: Permogorov Alexander , Cantono Giada , Guenot Diego , Persson Anders , Wahlström Claes-Göran 
Published: February 2022

AbstractOptimisation and reproducibility of beams of protons accelerated from laser-solid interactions require accurate control of a wide set of variables, concerning both the laser pulse and the target. Among the former ones, the chirp and temporal shape of the pulse reaching the experimental area may vary because of spectral phase modulations acquired along the laser system and beam transport. Here, we present an experimental study where we investigate the influence of the laser pulse chirp on proton acceleration from ultrathin flat foils (10 and 100 nm thickness), while minimising any asymmetry in the pulse temporal shape. The results show a $$\pm 10\%$$

±
10
%

change in the maximum proton energy depending on the sign of the chirp. This effect is most noticeable from 10 nm-thick target foils, suggesting a chirp-dependent influence of relativistic transparency.
CYP2B6 allelic variants and non-genetic factors influence CYP2B6 enzyme function
Authors: Mangó Katalin , Kiss Ádám Ferenc , Fekete Ferenc , Erdős Réka , Monostory Katalin 
Published: February 2022

AbstractHuman CYP2B6 enzyme although constitutes relatively low proportion (1–4%) of hepatic cytochrome P450 content, it is the major catalyst of metabolism of several clinically important drugs (efavirenz, cyclophosphamide, bupropion, methadone). High interindividual variability in CYP2B6 function, contributing to impaired drug-response and/or adverse reactions, is partly elucidated by genetic polymorphisms, whereas non-genetic factors can significantly modify the CYP2B6 phenotype. The influence of genetic and phenoconverting non-genetic factors on CYP2B6-selective activity and CYP2B6 expression was investigated in liver tissues from Caucasian subjects (N = 119). Strong association was observed between hepatic S-mephenytoin N-demethylase activity and CYP2B6 mRNA expression (P < 0.0001). In less than one third of the tissue donors, the CYP2B6 phenotype characterized by S-mephenytoin N-demethylase activity and/or CYP2B6 expression was concordant with CYP2B6 genotype, whereas in more than 35% of the subjects, an altered CYP2B6 phenotype was attributed to phenoconverting non-genetic factors (to CYP2B6-specific inhibitors and inducers, non-specific amoxicillin + clavulanic acid treatment and chronic alcohol consumption, but not to the gender). Furthermore, CYP2B6 genotype–phenotype mismatch still existed in one third of tissue donors. In conclusion, identifying potential sources of CYP2B6 variability and considering both genetic variations and non-genetic factors is a pressing requirement for appropriate elucidation of CYP2B6 genotype–phenotype mismatch.
Motor symptom asymmetry predicts non-motor outcome and quality of life following STN DBS in Parkinson's disease
Authors: Voruz Philippe , Pierce Jordan , Ahrweiller Kévin , Haegelen Claire , Sauleau Paul , Drapier Sophie , Drapier Dominique , Vérin Marc , Péron Julie 
Published: February 2022

AbstractRisk factors for long-term non-motor symptoms and quality of life following subthalamic nucleus deep brain stimulation (STN DBS) have not yet been fully identified. In the present study, we investigated the impact of motor symptom asymmetry in Parkinson’s disease. Data were extracted for 52 patients with Parkinson’s disease (half with predominantly left-sided motor symptoms and half with predominantly right-sided ones) who underwent bilateral STN and a matched healthy control group. Performances for cognitive tests, apathy and depression symptoms, as well as quality-of-life questionnaires at 12 months post-DBS were compared with a pre-DBS baseline. Results indicated a deterioration in cognitive performance post-DBS in patients with predominantly left-sided motor symptoms. Performances of patients with predominantly right-sided motor symptoms were maintained, except for a verbal executive task. These differential effects had an impact on patients’ quality of life. The results highlight the existence of two distinct cognitive profiles of Parkinson’s disease, depending on motor symptom asymmetry. This asymmetry is a potential risk factor for non-motor adverse effects following STN DBS.
Symbolic iteration method based on computer algebra analysis for Kepler’s equation
Authors: Zhang Ruichen , Bian Shaofeng , Li Houpu 
Published: February 2022

AbstractThe Kepler’s equation of elliptic orbits is one of the most significant fundamental physical equations in Satellite Geodesy. This paper demonstrates symbolic iteration method based on computer algebra analysis (SICAA) to solve the Kepler’s equation. The paper presents general symbolic formulas to compute the eccentric anomaly (E) without complex numerical iterative computation at run-time. This approach couples the Taylor series expansion with higher-order trigonometric function reductions during the symbolic iterative progress. Meanwhile, the relationship between our method and the traditional infinite series expansion solution is analyzed in this paper, obtaining a new truncation method of the series expansion solution for the Kepler’s equation. We performed substantial tests on a modest laptop computer. Solutions for 1,002,001 pairs of (e, M) has been conducted. Compared with numerical iterative methods, 99.93% of all absolute errors δE of eccentric anomaly (E) obtained by our method is lower than machine precision $$\epsilon$$
ϵ
over the entire interval. The results show that the accuracy is almost one order of magnitude higher than that of those methods (double precision). Besides, the simple codes make our method well-suited for a wide range of algebraic programming languages and computer hardware (GPU and so on).
Investigating the association between severity of COVID-19 infection during pregnancy and neonatal outcomes
Authors: Dileep Anita , ZainAlAbdin Sham , AbuRuz Salah 
Published: February 2022

AbstractPregnant women with COVID-19 require special attention and care, since the infection does not only affect the mother, but also her neonate and adversely affects pregnancy outcomes. The main goal of this retrospective cohort study is to investigate association between the maternal COVID-19 severity and risk of developing adverse neonatal outcomes. Patients were stratified into asymptomatic/mild and moderate to severe COVID-19. The following neonatal outcomes were assessed: gestational age at the time of delivery, birth weight, neonatal infection, neonatal intensive care unit (NICU) admission. The average age of patients was 28.5 ± 1.4 years old and majority were multigravida (74.0%, n = 148). Of total 200 pregnant women with COVID-19, 26.5% (n = 53) had moderate/severe disease and presented with higher incidence of preterm delivery and low birth weight (88.7%, n = 47; p < 0.001). In addition, more than half of the newborns delivered by mothers with severe disease were infected by SARS-COV-2 (58.5%, n = 31) and majority were admitted to the NICU (95.0%, n = 52). Based on the multivariate logistic regression analysis, pregnant women with moderate to severe COVID-19 were at much higher risk of preterm delivery, lower birth weight, neonatal infection, as well as neonatal ICU admission (p < 0.001). In addition, multigravida women were at higher risk for preterm delivery and lower birth weight (p = 0.017 and p = 0.02; respectively). Appropriate protective measures and early detection of suspected COVID-19 should be addressed for more favorable obstetric outcomes.
Choroidal vascularity index of patients with coronary artery disease
Authors: Seo Won-Woo , Yoo Hyo Soon , Kim Yong Dae , Park Sung Pyo , Kim Yong-Kyu 
Published: February 2022

AbstractWe investigated the changes in subfoveal choroidal thickness and choroidal vascularity index (CVI) and their relationship with the severity of coronary artery stenosis in patients with cardiovascular risk factors and symptoms suggestive of coronary artery disease (CAD). Ninety patients who underwent coronary angiography (CAG) for evaluation of their coronary artery status and cardiac symptoms were included. Forty-two patients showed no evidence of CAD; 31 patients had one to two vessel disease; and 17 had a triple vessel disease. There were no significant differences in the subfoveal choroidal thickness among the three groups; however, the CVI in the triple vessel disease group was lower than those in the other groups. The CVI values were good predictors of the presence of triple-vessel disease (p = 0.020). Multivariate logistic regression analysis results revealed that male sex (odds ratio 5.4, p = 0.049), hypertension (odds ratio 4.9, p = 0.017), and CVI (%, odds ratio 0.8, p = 0.016) were significant factors associated with the presence of triple vessel disease. Although CVI may not be a sensitive marker for detecting early changes in the coronary artery, it may be helpful in indicating severe CAD.
A video-based analysis of situations bearing the risk of respiratory disease transmission during football matches
Authors: Faude Oliver , Müller Simon , Schreiber Sebastian , Müller Jonas , Nebiker Lukas , Beaudouin Florian , Meyer Tim , Egger Florian 
Published: February 2022

AbstractWe aimed to analyze the number and type of contacts involving the risk of respiratory disease transmission during football match play. We analysed 50 matches from different playing levels. Two reviewers evaluated the contacts of all players in each match. We focused on between-player contacts, crowding, actions with potentially increased aerosol and droplet production and within-player hand-to-head contacts. We categorized the duels with direct contact into frontal and other ones and measured contact duration. The number of between-player contacts were similar between playing levels (median 28.3 [IQR 22.6, 33] contacts per player-hour). Frontal contacts summed up to 8% of all contacts. Contacts involving the head occurred less than once per player and match with none lasting longer than 3 s. Crowding included between two and six players and the duration was mostly less than 10 s. Aerosol and droplet producing activities were three to four times more frequent in adult compared to youth players. Our results suggest that the risk of respiratory pathogen transmission is low during football matches. This conclusion is based on the finding that most close contact situations are of short duration and on the fact that it is an outdoor sport.
The insecticide β-Cyfluthrin induces acute arrhythmic cardiotoxicity through interaction with NaV1.5 and ranolazine reverses the phenotype
Authors: da Silva Maria Vitoria , Santos-Miranda Artur , Joviano-Santos Julliane V. , Souza Diego Santos , Marques Leisiane Pereira , Sarmento Jaqueline Oliveira , Beserra Samuel Santos , Roman-Campos Danilo 
Published: February 2022

Abstract
β-Cyfluthrin, a class II Pyrethroid, is an insecticide used worldwide in agriculture, horticulture (field and protected crops), viticulture, and domestic applications. β-Cyfluthrin may impair the function of biological systems; however, little information is available about its potential cardiotoxic effect. Here, we explored the acute toxicity of β-Cyfluthrin in isolated heart preparations and its cellular basis, using isolated cardiomyocytes. Moreover, β-Cyfluthrin effects on the sodium current, especially late sodium current (INa-L), were investigated using human embryonic kidney cells (HEK-293) cells transiently expressing human NaV1.5 channels. We report that β-Cyfluthrin raised INa-L in a dose-dependent manner. β-Cyfluthrin prolonged the repolarization of the action potential (AP) and triggered oscillations on its duration. Cardiomyocytes contraction and calcium dynamics were disrupted by the pesticide with a marked incidence of non-electronic-stimulated contractions. The antiarrhythmic drug Ranolazine was able to reverse most of the phenotypes observed in isolated cells. Lastly, ventricular premature beats (VPBs) and long QT intervals were found during β-Cyfluthrin exposure, and Ranolazine was able to attenuate them. Overall, we demonstrated that β-Cyfluthrin can cause significant cardiac alterations and Ranolazine ameliorated the phenotype. Understanding the insecticides’ impacts upon electromechanical properties of the heart is important for the development of therapeutic approaches to treat cases of pesticides intoxication.
Tell or Retell? The Role of Task and Language in Spanish–English Narrative Microstructure Performance
Authors: Wofford Mary Claire , Cano Jessica , Goodrich J. Marc , Fitton Lisa 
Published: February 2022

Purpose:
This study examined performance of dual language learners (DLLs) on Spanish- and English-language narrative story retells and unique tells. Transcription and analysis focused on comparisons of common microstructural language sample measures in Spanish and English across tasks. Each language sample measure was evaluated for its possible convergence with norm-referenced standardized assessments for DLL children.

Method:

Spanish–English DLLs (
N
= 133) enrolled in English-only kindergarten or first-grade classrooms completed two-language sample tasks (one in each language), which were transcribed and analyzed using Systematic Analysis of Language Transcripts (Miller & Iglesias, 2017) for measures of syntactic complexity (mean length of utterance [MLU] in words), lexical diversity (number of different words [NDW]), and grammaticality (percent grammatical utterances [PGU]). Students also completed a norm-referenced sentence repetition task (Peña et al., 2014) and expressive vocabulary assessment (Martin, 2013).

Results:
Comparison of story retells and unique stories revealed similar performance on MLU, NDW, and PGU across elicitation techniques, with one exception: NDW in Spanish was higher in the story retell condition. Predictive models revealed several differences in the relations between the microstructure measures and norm-referenced language measures by elicitation technique, although neither context demonstrated a consistent advantage across all metrics.

Conclusions:
Measures derived from story retells and unique tells offer practical findings for speech-language pathologists and other educators to use in assessment of early grade DLLs. This work increases knowledge of procedural differences across narrative assessments and their influence on language variables, supporting school-based speech-language pathologists in making assessment decisions for DLLs on their caseload.

Supplemental Material:

https://doi.org/10.23641/asha.19191278