bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coupling of Physical and Chromatographic Separation for Analyzing Cream Containing Miconazole, Mometasone Furoate and Gentamicin Sulphate
Authors: Tawakkol Shereen Mostafa , Fayez Yasmin Mohammed , Fahmy Nesma Mahmoud , Lotfy Hayam Mahmoud , Shehata Mostafa Abdel-Atty 
Published: July 2022

Abstract
High-performance liquid chromatographic method was developed, validated and applied for miconazole, mometasone and gentamicin in Momenta® cream. Physical separation was applied using two extraction procedures due to different solubility of the three components. First, a methanolic extract of the cream contained miconazole and mometasone was chromatographed on ODS-3 Inertsil C18 column (150 × 4.6, 5 μm) using acetonitrile: water (80:20, v/v) as a mobile phase, flow rate 1.5 mL·min−1, scanned at 230 nm, showing tR 2.817 and 6.808 min for mometasone and miconazole, respectively. Second, an aqueous extract of the cream containing gentamicin was derivatized with o-phthalaldehyde in order to enhance the gentamicin UV detection and subjected to ion pairing chromatography on Inertsil ODS-3 C18 column (150 × 4.6, 5 μm), using methanol: 0.025 M heptane sulphonic acid: glacial acetic acid (75:20:5, by volume) as a mobile phase, flow rate 0.8 mL·min−1, scanned at 330 nm where the three active gentamicin isomers were separated at tR 11.7, 15.6 and 18.3 min. Suitability of this method for quantitative estimation of the drugs was proved by validation according to ICH guidelines. The method was selective, precise and accurate so could be used for analysis of cream formulation in QC labs.
Patient-reported outcomes in the aging population of adults with congenital heart disease: results from APPROACH-IS
Authors: Moons Philip , Luyckx Koen , Thomet Corina , Budts Werner , Enomoto Junko , Sluman Maayke A , Yang Hsiao-Ling , Jackson Jamie L , Khairy Paul , Cook Stephen C , Chidambarathanu Shanthi , Alday Luis , Oechslin Erwin , Eriksen Katrine , Dellborg Mikael , Berghammer Malin , Johansson Bengt , Mackie Andrew S , Menahem Samuel , Caruana Maryanne , Veldtman Gruschen , Soufi Alexandra , Fernandes Susan M , White Kamila , Callus Edward , Kutty Shelby , Kovacs Adrienne H ,   the APPROACH-IS consortium, Alday Luis , Maisuls Héctor , Vega Betina , Menahem Samuel , Eaton Sarah , Wang Qi Feng , Larion Ruth , Budts Werner , Deyk Kristien Van , Apers Silke , Goossens Eva , Rassart Jessica , Luyckx Koen , Moons Philip , Rempel Gwen , Mackie Andrew , Ballantyne Ross , Rankin Kathryn , Norris Colleen , Taylor Dylan , Vondermuhll Isabelle , Windram Jonathan , Heggie Pamela , Lasiuk Gerri , Khairy Paul , Proietti Anna , Dore Annie , Mercier Lise-Andrée , Mongeon François-Pierre , Marcotte François , Ibrahim Reda , Mondésert Blandine , Côté Marie-Claude , Kovacs Adrienne , Oechslin Erwin , Bandyopadhyay Mimi , Soufi Alexandra , Filippo Sylvie Di , Sassolas François , Bozio André , Chareyras Cécile , Chidambarathanu Shanthi , Farzana Farida , Lakshmi Nitya , Callus Edward , Quadri Emilia , Chessa Massimo , Campioni Giovanna , Giamberti Alessandro , Enomoto Junko , Mizuno Yoshiko , Caruana Maryanne , Grech Victor , Vella Sheena , Mifsud Anabel , Borg Neville , Chircop Daniel , Balbi Matthew Mercieca , Critien Rachel Vella , Farrugia James , Gatt Yanika , Muscat Darlene , Eriksen Katrine , Estensen Mette-Elise , Dellborg Mikael , Berghammer Malin , Mattsson Eva , Strandberg Anita , Karlström-Hallberg Pia , Johansson Bengt , Kronhamn Anna-Karin , Schwerzman Markus , Thomet Corina , Huber Margrit , Wang Jou-Kou , Lu Chun-Wei , Yang Hsiao-Ling , Hua Yu Chuan , Mulder Barbara , Sluman Maayke , Post Marco , Pieper Els , Peels Kathinka , Waskowsky Marc , Veldtman Gruschen , Faust Michelle , Lozier Colin , Reed Christy , Hilfer Jamie , Daniels Curt , Jackson Jamie , Kutty Shelby , Chamberlain Carolyn , Warta Sara , Cook Stephen , Hindes Morgan , Cedars Ari , White Kamila , Fernandes Susan , Romfh Anitra , MacMillen Kirstie 
Published: July 2022

Abstract
The congenital heart disease (CHD) population now comprises an increasing number of older persons in their 6th decade of life and beyond. We cross-sectionally evaluated patient-reported outcomes (PROs) in persons with CHD aged 60 years or older, and contrasted these with PROs of younger patients aged 40–59 years and 18–39 years. Adjusted for demographic and medical characteristics, patients ≥60 years had a lower Physical Component Summary, higher Mental Component Summary, and lower anxiety (Hospital Anxiety and Depression Scale-Anxiety) scores than patients in the two younger categories. For satisfaction with life, older persons had a higher score than patients aged 40–59 years.
Registration: ClinicalTrials.gov NCT02150603.
Heterologous biosynthesis of taraxerol by engineered Saccharomyces cerevisiae
Authors: Tan Jinxiu , Zhang Chuanbo , Pai Huihui , Lu Wenyu 
Published: July 2022

Abstract
Taraxerol is an oleanane-type pentacyclic triterpenoid compound distributed in many plant species that has good effects on the treatment of inflammation and tumors. However, the taraxerol content in medicinal plants is low, and chemical extraction requires considerable energy and time, so taraxerol production is a problem. It is a promising strategy to produce taraxerol by applying recombinant microorganisms. In this study, a Saccharomyces cerevisiae strain WKde2 was constructed to produce taraxerol with a titer of 1.85 mg·l–1, and the taraxerol titer was further increased to 12.51 mg·l–1 through multiple metabolic engineering strategies. The endoplasmic reticulum (ER) size regulatory factor INO2, which was reported to increase squalene and cytochrome P450-mediated 2,3-oxidosqualene production, was overexpressed in this study, and the resultant strain WTK11 showed a taraxerol titer of 17.35 mg·l–1. Eventually, the highest reported titer of 59.55 mg·l–1 taraxerol was achieved in a 5 l bioreactor. These results will serve as a general strategy for the production of other triterpenoids in yeast.
Individual differences and compliance intentions with COVID-19 restrictions: insights from a lockdown in Melbourne (Australia)
Authors: Auton Jaime C , Sturman Daniel 
Published: July 2022

Abstract
The coronavirus (COVID-19) pandemic has caused an international public health and economic crisis. Despite the COVID-19 vaccine rollout in many countries from late 2020, non-pharmaceutical interventions are still required to minimize the spread of the virus. However, notable variation in voluntary compliance with these interventions has been reported. This study investigated various individual differences associated with intentions to comply with COVID-19 restrictions during a sustained (112 day) lockdown in Melbourne (Australia) in late 2020. Participants (N = 363) completed an online survey where they responded to various socio-demographic, health and psychological questions. Participants also responded to a series of vignettes that assessed their intended behaviour in specific situations and their knowledge of the current COVID-19 restrictions. Overall, it was found that greater levels of organization predicted greater intentions to comply with the COVID-19 restrictions, while higher socio-economic status, sociability and anxiety predicted lower compliance intentions. Further, individuals previously diagnosed with COVID-19 reported lower intentions to comply with the COVID-19 restrictions. The strongest predictor of compliance intentions, however, was a greater knowledge of the current restrictions. These findings highlight that public health orders around COVID-19 restrictions should be presented in a clear and uncomplicated manner and should target specific groups to increase compliance.
Barriers to health promotion strategy work in Finnish municipalities
Authors: Leinonen Jaana , Syväjärvi Antti 
Published: July 2022

AbstractThis study’s aim is to increase insights into the characteristics and conditions of municipal health promotion strategy work. Specifically, we aim to find barriers connected to strategy work, that is, creating and formulating municipal health promotion strategy documents, with the following empirical research question: What perceptions do municipal health promotion strategy actors have on barriers connected to low citizen participation, narrow cross-departmental collaboration and weak goal orientation in health promotion strategy work? Empirical data comprised 22 thematics, individual interviews collected from municipal managers, sector managers and municipal health coordinators. Analysis was conducted using content analysis. This study’s findings showed that the main barriers impeding optimal strategy work conditions were administrators’ sceptical views of participation and undeveloped administrative practices in inclusive citizen participation, inadequate leadership to unite actors and facilitate collaborative strategy work practices, and challenges in integrating various views into coherent strategic goals. Also, a need for boundary-spanning leadership that strengthens mutual interaction and creates a shared understanding of health promotion to build strategic collaboration and goal-oriented strategy work was revealed. Finally, to strengthen this leadership, influencing administrators’ mindsets, behaviour and administrative culture is imperative.
Involvement of histone deacetylase CsHDA2 in regulating (E)-nerolidol formation in tea (Camellia sinensis) exposed to tea green leafhopper infestation
Authors: Gu Dachuan , Wu Shuhua , Yu Zhenming , Zeng Lanting , Qian Jiajia , Zhou Xiaochen , Yang Ziyin 
Published: July 2022

Abstract
Herbivore-induced plant volatiles (HIPVs) help the tea plant (Camellia sinensis) adapt to environmental stress, and they are also quality-related components of tea. However, the upstream mechanism regulating the herbivore-induced expression of volatile biosynthesis genes is unclear, especially at the level of epigenetic regulation. In this study, similar to the effects of a tea green leafhopper infestation, treatments with exogenous jasmonic acid (JA) and histone deacetylase inhibitors significantly increased the (E)-nerolidol content in tea and induced the expression of the associated biosynthesis gene CsNES. Furthermore, a key transcription factor related to JA signaling, myelocytomatosis 2 (CsMYC2), interacted with histone deacetylase 2 (CsHDA2) in vitro and in vivo. A tea green leafhopper infestation inhibited CsHDA2 expression and decreased CsHDA2 abundance. Moreover, the tea green leafhopper infestation increased H3 and H4 acetylation levels in the promoter region of CsNES, which in turn upregulated the expression of CsNES and increased the (E)-nerolidol content. In this study, we revealed the effects of histone acetylations on the accumulation of HIPVs, while also confirming that CsHDA2–CsMYC2 is an important transcriptional regulatory module for the accumulation of (E)-nerolidol induced by tea green leafhoppers. The results of this study may be useful for characterizing plant aromatic compounds and the main upstream stress-responsive signaling molecules. Furthermore, the study findings will assist researchers clarify the epigenetic regulation influencing plant secondary metabolism in response to external stress.
The use of objective assessments in the evaluation of technical skills in cardiothoracic surgery: a systematic review
Authors: Hussein Nabil , Van den Eynde Jef , Callahan Connor , Guariento Alvise , Gollmann-Tepeköylü Can , Elbatarny Malak , Loubani Mahmoud 
Published: July 2022

Summary

OBJECTIVES
With reductions in training time and intraoperative exposure, there is a need for objective assessments to measure trainee progression. This systematic review focuses on the evaluation of trainee technical skill performance using objective assessments in cardiothoracic surgery and its incorporation into training curricula.

METHODS
Databases (EBSCOHOST, Scopus and Web of Science) and reference lists of relevant articles for studies that incorporated objective assessment of technical skills of trainees/residents in cardiothoracic surgery were included. Data extraction included task performed; assessment setting and tool used; number/level of assessors; study outcome and whether the assessments were incorporated into training curricula. The methodological rigour of the studies was scored using the Medical Education Research Study Quality Instrument (MERSQI).

RESULTS
Fifty-four studies were included for quantitative synthesis. Six were randomized-controlled trials. Cardiac surgery was the most common speciality utilizing objective assessment methods with coronary anastomosis the most frequently tested task. Likert-based assessment tools were most commonly used (61%). Eighty-five per cent of studies were simulation-based with the rest being intraoperative. Expert surgeons were primarily used for objective assessments (78%) with 46% using blinding. Thirty (56%) studies explored objective changes in technical performance with 97% demonstrating improvement. The other studies were primarily validating assessment tools. Thirty-nine per cent of studies had established these assessment tools into training curricula. The mean ± standard deviation MERSQI score for all studies was 13.6 ± 1.5 demonstrating high validity.

CONCLUSIONS
Despite validated technical skill assessment tools being available and demonstrating trainee improvement, their regular adoption into training curricula is lacking. There is a need to incorporate these assessments to increase the efficiency and transparency of training programmes for cardiothoracic surgeons.
Simplicity of Higher Rank TripletW-Algebras
Authors: Sugimoto Shoma 
Published: July 2022

AbstractWe show that the higher rank triplet $W$-algebra $W_{\sqrt {p}Q}$ is simple for $p\geq h-1$. Furthermore, we show that the $W_{\sqrt {p}Q}$-module $W_{\sqrt {p}(Q-\lambda _0)+\lambda _p}$ introduced in [21] is simple if $\sqrt {p}\lambda _p$ is in the closure of the fundamental alcove and give the decomposition as a direct sum of simple $\boldsymbol {W}_{p-h}({\mathfrak {g}})$-modules.
Sharp Bound for the Fourth Moment of Holomorphic Hecke Cusp Forms
Authors: Zenz Peter 
Published: July 2022

Abstract
We prove that the fourth moment of holomorphic Hecke cusp forms is bounded by a constant provided that the Generalized Riemann Hypothesis holds. We accomplish this using Watson’s formula, which translates the question in hand into a moment problem for $L$-functions that is amenable to the techniques of Soundararajan and Harper on obtaining sharp bounds for moments of the Riemann zeta function.
The Virasoro-Like Algebra of a Frobenius Manifold
Authors: Liu Si-Qi , Yang Di , Zhang Youjin , Zhou Jian 
Published: July 2022

Abstract
For an arbitrary calibrated Frobenius manifold, we construct an infinite dimensional Lie algebra, called the Virasoro-like algebra, which is a deformation of the Virasoro algebra of the Frobenius manifold. By using the Virasoro-like algebra we give a family of quadratic PDEs that are satisfied by the genus-zero free energy of the Frobenius manifold. We also derive, under the semisimplicity assumption, the Virasoro constraints for the corresponding abstract Hodge partition function.
A hybrid model to study how late long-term potentiation is affected by faulty molecules in an intraneuronal signaling network regulating transcription factor CREB
Authors: Emadi Ali , Ozen Mustafa , Abdi Ali 
Published: July 2022

Abstract
Systems biology analysis of intracellular signaling networks has tremendously expanded our understanding of normal and diseased cell behaviors and has revealed paths to finding proper therapeutic molecular targets. When it comes to neurons in the human brain, analysis of intraneuronal signaling networks provides invaluable information on learning, memory and cognition-related disorders, as well as potential therapeutic targets. However, neurons in the human brain form a highly complex neural network that, among its many roles, is also responsible for learning, memory formation and cognition. Given the impairment of these processes in mental and psychiatric disorders, one can envision that analyzing interneuronal processes, together with analyzing intraneuronal signaling networks, can result in a better understanding of the pathology and, subsequently, more effective target discovery. In this paper, a hybrid model is introduced, composed of the long-term potentiation (LTP) interneuronal process and an intraneuronal signaling network regulating CREB. LTP refers to an increased synaptic strength over a long period of time among neurons, typically induced upon occurring an activity that generates high-frequency stimulations (HFS) in the brain, and CREB is a transcription factor known to be highly involved in important functions of the cognitive and executive human brain such as learning and memory. The hybrid LTP-signaling model is analyzed using a proposed molecular fault diagnosis method. It allows to study the importance of various signaling molecules according to how much they affect an intercellular phenomenon when they are faulty, i.e. dysfunctional. This paper is intended to suggest another angle for understanding the pathology and therapeutic target discovery by classifying and ranking various intraneuronal signaling molecules based on how much their faulty behaviors affect an interneuronal process. Possible relations between the introduced hybrid analysis and the previous purely intracellular analysis are investigated in the paper as well.
HIV-1 subtype F integrase polymorphisms external to the catalytic core domain contribute to severe loss of replication capacity in context of the integrase inhibitor resistance mutation Q148H
Authors: Aulicino Paula C , Momin Zoha , Rozenszajn Mijael , Monzon Arturo , Arazi-Caillaud Solange , Bologna Rosa , Mangano Andrea , Kimata Jason T 
Published: July 2022

AbstractBackgroundIn prior studies, HIV-1 BF recombinants with subtype F integrases failed to develop resistance to raltegravir through the Q148H mutational pathway. We aimed to determine the role of subtype-specific polymorphisms in integrase on drug susceptibility, viral replication and integration.MethodsIntegrase sequences were retrieved from the Los Alamos Database or obtained from the Garrahan HIV cohort. HIV-1 infectious molecular clones with or without Q148H (+ G140S) resistance mutations were constructed using integrases of subtype B (NL4-3) or F1(BF) ARMA159 and URTR23. Integrase chimeras were generated by reciprocal exchanges of a 200 bp fragment spanning amino acids 85–150 of the catalytic core domain (CCD) of NL4-3-Q148H and either ARMA159-Q148H or URTR23-Q148H. Viral infections were quantified by p24 ELISA and Alu-gag integration PCR assay.ResultsAt least 18 different polymorphisms distinguish subtype B from F1(BF) recombinant integrases. In phenotypic experiments, p24 at Day 15 post-infection was high (105–106 pg/mL) for WT and NL4-3-Q148H; by contrast, it was low (102–104 pg/mL) for both F1(BF)-Q148H + G140S viruses, and undetectable for the Q148H mutants. Compared with WT viruses, integrated DNA was reduced by 5-fold for NL4-3-Q148H (P = 0.05), 9-fold for URTR23-Q148H (P = 0.01) and 16000-fold for ARMA159-Q148H (P = 0.01). Reciprocal exchange between B and F1(BF) of an integrase CCD region failed to rescue the replicative defect of F1(BF) integrase mutants.ConclusionsThe functional impairment of Q148H in the context of subtype F integrases from BF recombinants explains the lack of selection of this pathway in vivo. Non-B polymorphisms external to the integrase CCD may influence the pathway to integrase strand transfer inhibitor resistance.
Advanced Spectrophotometric Resolution Techniques for a Spectrally Overlapping Spasmolytic Binary Mixture along with Dichloroaniline as a Toxic Impurity: Application to Content Uniformity Testing
Authors: Darweish Eman , Marzouk Hoda M , Fayez Yasmin M , Eissa Maya S 
Published: July 2022

Abstract

Background
Irritable bowel syndrome (IBS) is a common disorder leading to undesirable pain. Phloroglucinol (PHG) and trimethylphloroglucinol (TMG) are co-formulated as spasmolytic medication that is considered to be effective in reducing smooth muscle spasm. 3,5-Dichloroaniline (DCL) is a specified PHG pharmacopoeial impurity which needs to be monitored to avoid its toxic effects.

Objective
Different smart approaches are presented to provide simple, reliable, and economic spectrophotometric methods able to resolve the severe overlap in the spectra of PHG and TMG in their pure and pharmaceutical forms, in addition to their estimation in the presence of DCL as a toxic impurity of PHG without any need for initial separation.

Methods
The presented work includes univariate methods, derivative ratio (DR), ratio difference (RD), mean centering (MCR) and deconvulated Fourier method (DF), which were able to determine PHG and TMG simultaneously in their binary mixture. Firstly, DCL was estimated in the zero order, where the two drugs have zero absorption at 247.0 nm, and then its contribution was eliminated by applying ratio subtraction method. Multivariate chemometric partial least squares (PLS) and principal component regression (PCR) models were also applied to determine PHG and TMG simultaneously in the presence of the impurity, DCL

Results
Univariate methods were applied in the range 5.0–30.0, 2.5–25.0, and 1.0–12.0 µg/mL for PHG, TMG, and DCL, respectively. The proposed chemometric models were used in the range 6.0–14.0, 5.0–25.0 and 2.0–10.0 µg/mL for PHG, TMG, and DCL, respectively. These analytical approaches succeeded in estimating the cited drugs in their pharmaceutical formulation and assessing content uniformity of dosage units. The methods were statistically compared with a reported HPLC method, and the results revealed no significance statistical difference.

Conclusion
This work provides for the first time successful univariate and multivariate PLS and PCR methods to assess PHG and TMG in the presence of DCL as a toxic impurity along with content uniformity testing of dosage units.

Highlights
Comparative univariate and multivariate spectrophotometric analytical approaches are presented, for the first time, for estimation of spasmolytic formulation of PHG and TMG in the presence of DCL as a PHG toxic impurity. Successful application to content uniformity testing of Stopspasm® dosage form is demonstrated. A statistical study, including t-tests and one-way analysis of variance (ANOVA), was conducted.