bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decolorization of soybean oil by acid‐activated Ca‐Bentonite (EL Gnater, Central Tunisia)
Authors: Chakroun Salima , Sghaier Dalel , Gaied Mohamed Essghaier 
Published: October 2022

AbstractIn the central Atlas of Tunisia, numerous outcrops of the upper Eocene provide smectitic clays. A representative sample (A) which was collected from the El Gnater region round El Ala underwent mineralogical and physicochemical analyses (granulometry, DRX, BET, FT‐IR, etc.). This clay showing percentage of the order of 80% in smectite were activated with hydrochloric acid to improve its physicochemical properties, and consequently to increase their decolorization power for soybean oils. The best results were obtained using clay, activated with 3 N hydrochloric acid for 3 h at 75°C (AA2). Indeed, the specific surface area increased from 44 to 119 m2/g. The characterization of physicochemical properties was carried out on the samples (A: raw, AA: activated at different acid concentrations 1.5, 3 and 4.5 N at 75°C during 3 h). Under optimal conditions, the decolorization power of AA2 clay (60.15%) was found to be better than that obtained by a bleaching earth imported from Germany (57.27%). These encouraging results allow the reduction or permanently stopping of these materials imports.
Recent advances and potential applications of flexible adsorption and separation materials: A review
Authors: Sun Qi‐Meng , He Jing‐Hui , Lu Jian‐Mei 
Published: October 2022

AbstractIn adsorption processes of the liquid or gas phase, a particular dynamic adsorption behavior caused by the reversible mechanical deformation (expansion or contraction) of absorption materials driven by the host–guest interaction is called “flexible adsorption,” and the materials with this particular behavior can be called “flexible adsorption materials.” Flexible absorption materials, such as crosslinked polymers, have received much attention from researchers due to their flexible framework structure and specific adsorption kinetics. They have shown great potential in gas storage and other energy applications. This paper reviews recent advances in the definition, theoretical development, and classification of flexible absorption materials. Potential application examples of flexible absorption materials in gas/liquid absorption and separation, functional devices, and energy catalysis are analyzed to guide their development.
Impact of upstream reservoirs on geomorphic evolution in the middle and lower reaches of the Yangtze River
Authors: Yang Yunping , Zhou Liangping , Zhu Lingling , Liu Wanli , Wang Jianjun 
Published: October 2022

AbstractIntensive human activities in river basins have substantial effects on fluvial hydrological and morphological processes and developmental trends. In this study, we assessed the evolutionary characteristics and developmental trends of the fluvial morphological system of the Three Gorges Dam (TGD) downstream to understand the impacts of human activities on the fluvial hydrological–morphological system of the region. The results indicated that the Three Gorges Project (TGP) and other upstream cascade reservoirs promoted sediment retention, which in turn aggravated the trend of declining sediment load and cumulative scouring over a long distance in the channels downstream of the TGD. Approximately 84% of scouring occurred in the low‐water channels, along with thalweg scouring and riverbank collapse. Notably, extended operation of the TGP and other upstream cascade reservoirs has resulted in gradual changes in the downstream riverbeds. Specifically, we found that the most intensive scouring zone shifted downstream from the near‐dam Yichang–Chenglingji reach (0–408 km) to the Chenglingji–Hankou reach. The scouring–silting distribution in swales has also changed: the scouring of deep swales and silting on floodplains prior to the TGP have been replaced by the scouring of both deep swales and floodplains. The scouring intensity of riverbeds in the Yichang–Hukou reach (954 km) was slightly higher than that forecasted prior to the TGP; the scouring intensities increased significantly during 2013–2021, which could be attributed to the TGP and other upstream cascade reservoirs; river sand mining and waterway dredging are secondary factors. We conclude that, owing to the effects of sediment retention by cascade reservoirs in the upper reaches of the Yangtze River (or Changjiang River), the cumulative scouring intensities of riverbeds in the middle and lower reaches of the Yangtze River will continue to increase in future decades.
Competitive evolved sub‐clonal BCR::ABL1 and novel MSI2::PC fusion genes in myelodysplastic syndrome with isolated del(5q)
Authors: Zhang Yanqing , Liu Yang , Wang Tong , Wang Hui , Chen Xue , Cao Panxiang , Ma Xiaoli , Liu Mingyue , Xu Ping , Bi Hailiang , Pan Jiaqi , Jiang Yongfang , Li Xiaoyun , Wang Wei , Liu Hongxing 
Published: October 2022

AbstractMyelodysplastic syndrome (MDS) represents a group of neoplasms with extensive heterogeneity. Recurrent mutations in dozens of driver genes have been identified in over 90% of MDS cases, although fusion genes are rarely seen. We first report the competitive evolved sub‐clonal breakpoint cluster region (BCR)::ABL1 and novel MSI2::PC fusion gene in MDS with del(5q) in initial diagnosis that underwent dismal progression. However, the BCR::ABL1 clone vanished while the MSI2::PC clone rose to the major one with disease progression. A novel MSI2::PC fusion transcript was identified in initial diagnosis and disease progression of the patient through transcriptome sequencing (RNA‐seq) and Quantitative reverse transcription polymerase Chain Reaction (PCR) showed MSI2::PC/ABL1 expression at initial diagnosis and disease progression. In addition, mutation screening of 300 leukemia driver genes identified ARID2 c.5046del/p.F1682Lfs*19 and ZNF292 c.4565A > G/p.Q1522R mutation in bone marrow sample at initial diagnosis and disease progression. In conclusion, the dynamic process of the two fusion and phenotype manifestations may help to understand further the molecular significance of the anomalies of BCR::ABL1, MSI2, and PC in oncogenesis.
RNPS1 functions as an oncogenic splicing factor in cervical cancer cells
Authors: Deka Bhagyashree , Chandra Pratap , Yadav Priyanka , Rehman Ayushi , Kumari Sweta , Kunnumakkara Ajaikumar B. , Singh Kusum Kumari 
Published: October 2022

AbstractNumerous recent studies suggest that cancer‐specific splicing alteration is a critical contributor to the pathogenesis of cancer. RNA‐binding protein with serine‐rich domain 1, RNPS1, is an essential regulator of the splicing process. However, the defined role of RNPS1 in tumorigenesis still remains elusive. We report here that the expression of RNPS1 is higher in cervical carcinoma samples from The Cancer Genome Atlas (TCGA‐cervical squamous cell carcinoma and endocervical adenocarcinoma) compared to the normal tissues. Consistently, the expression of RNPS1 was high in cervical cancer cells compared to a normal cell line. This study shows for the first time that RNPS1 promotes cell proliferation and colony‐forming ability of cervical cancer cells. Importantly, RNPS1 positively regulates migration‐invasion of cervical cancer cells. Intriguingly, depletion of RNPS1 increases the chemosensitivity against the chemotherapeutic drug doxorubicin in cervical cancer cells. Further, we characterized the genome‐wide isoform switching stimulated by RNPS1 in cervical cancer cell. Mechanistically, RNA‐sequencing analysis showed that RNPS1 regulates the generation of tumor‐associated isoforms of key genes, particularly Rac1b, RhoA, MDM4, and WDR1, through alternative splicing. RNPS1 regulates the splicing of Rac1 pre‐mRNA via a specific alternative splicing switch and promotes the formation of its tumorigenic splice variant, Rac1b. While the transcriptional regulation of RhoA has been well studied, the role of alternative splicing in RhoA upregulation in cancer cells is largely unknown. Here, we have shown that the knockdown of RNPS1 in cervical cancer cells leads to the skipping of exons encoding the RAS domain of RhoA, consequently causing decreased expression of RhoA. Collectively, we conclude that the gain of RNPS1 expression may be associated with tumor progression in cervical carcinoma. RNPS1‐mediated alternative splicing favors an active Rac1b/RhoA signaling axis that could contribute to cervical cancer cell invasion and metastasis. Thus, our work unveils a novel role of RNPS1 in the development of cervical cancer.
Controlling collagen gelation pH to enhance biochemical, structural, and biomechanical properties of tissue‐engineered menisci
Authors: Kim Jongkil , Bonassar Lawrence J. 
Published: October 2022

AbstractCollagen‐based hydrogels have been widely used in biomedical applications due to their biocompatibility. Enhancing mechanical properties of collagen gels remains challenging while maintaining biocompatibility. Here, we demonstrate that gelation pH has profound effects on cellular activity, collagen fibril structure, and mechanical properties of the fibrochondrocyte‐seeded collagen gels in both short‐ and long‐terms. Acidic and basic gelation pH, below pH 7.0 and above 8.5, resulted in dramatic cell death. Gelation pH ranging from 7.0 to 8.5 showed a relatively high cell viability. Furthermore, physiologic gelation (pH 7.5) showed the greatest collagen deposition while glycosaminoglycan deposition appeared independent of gelation pH. Scanning electron microscopy showed that neutral and physiologic gelation pH, 7.0 and 7.5, exhibited well‐aligned collagen fibril structure on day 0 and enhanced collagen fibril structure with laterally joined fibrils on day 30. However, basic pH, 8.0 and 8.5, displayed a densely packed collagen fibril structure on day 0, which was also persistent on day 30. Initial equilibrium modulus increased with increasing gelation pH. Notably, after 30 days of culture, gelation pH of 7.5 and 8.0 showed the highest equilibrium modulus, reaching 150 –160 kPa. While controlling gelation pH is simply achieved compared with other strategies to improve mechanical properties, its influences on biochemical and biomechanical properties of the collagen gel are long‐lasting. As such, gelation pH is a useful means to modulate both biochemical and biomechanical properties of the collagen‐based hydrogels and can be utilized for diverse types of tissue engineering due to its simple application.
The peritumoral brain zone in glioblastoma: where we are and where we are going
Authors: Giambra Martina , Di Cristofori Andrea , Valtorta Silvia , Manfrellotti Roberto , Bigiogera Vittorio , Basso Gianpaolo , Moresco Rosa Maria , Giussani Carlo , Bentivegna Angela 
Published: October 2022

AbstractGlioblastoma (GBM) is the most aggressive and invasive primary brain tumor. Current therapies are not curative, and patients' outcomes remain poor with an overall survival of 20.9 months after surgery. The typical growing pattern of GBM develops by infiltrating the surrounding apparent normal brain tissue within which the recurrence is expected to appear in the majority of cases. Thus, in the last decades, an increased interest has developed to investigate the cellular and molecular interactions between GBM and the peritumoral brain zone (PBZ) bordering the tumor tissue. The aim of this review is to provide up‐to‐date knowledge about the oncogenic properties of the PBZ to highlight possible druggable targets for more effective treatment of GBM by limiting the formation of recurrence, which is almost inevitable in the majority of patients. Starting from the description of the cellular components, passing through the illustration of the molecular profiles, we finally focused on more clinical aspects, represented by imaging and radiological details. The complete picture that emerges from this review could provide new input for future investigations aimed at identifying new effective strategies to eradicate this still incurable tumor.
We need to build the evidence: A systematic review of psychological first aid on mental health and well‐being
Authors: Hermosilla Sabrina , Forthal Sarah , Sadowska Karolina , Magill Elizabeth B. , Watson Patricia , Pike Kathleen M. 
Published: October 2022

AbstractEnsuring effective mental health and psychosocial support is crucial following exposure to a potentially traumatic event and can have long‐term consequences for individuals, families, and communities. Psychological first aid (PFA) has become a widespread intervention of choice following exposure to conflict or disaster; however, its impact is unknown. This systematic review assessed PFA efficacy in improving the mental health and psychosocial well‐being of individuals exposed to potentially traumatic events. We searched PubMed, PsycINFO, PTSDpubs, and EMBASE for peer reviewed studies evaluating programmatic outcomes of PFA, or an adapted intervention, published in English before March 9, 2021. Studies evaluating training outcomes or program feasibility were excluded. The primary outcomes were reported measures of participant mental health and psychosocial well‐being, with narrative results presented for each. The Cochrane Risk of Bias tool was applied. Of 9,048 potentially eligible citations, 12 studies with a total of 1,437 participants met the inclusion criteria. Only one study was a randomized controlled trial. The findings from all studies suggest a positive impact of PFA, with most reporting reduced symptoms of anxiety, depression, posttraumatic stress, and distress, as well as improved ratings of mood, the experience of safety, connectedness, and a sense of control, among youth and adults. Risk of bias was generally high. Inconsistent intervention components, insufficient evaluation methodologies, and a high risk of bias within the reviewed studies present challenges in assessing PFA efficacy, and an imbalance between popular support for PFA and scant evidence of outcome data exists. Further research is needed to justify the proliferation of PFA.
Spindle Cell Carcinoma of the Head and Neck: Clinical Characteristics and Molecular Signatures
Authors: Lin Mei‐Chun , Hsu Chia‐Lang , Lai Shih‐Fan , Huang Yen‐Lin , Hsieh Min‐Shu , Chen Tseng‐Cheng , Chen Chun‐Nan , Wang Cheng‐Ping , Yang Tsung‐Lin , Ko Jenq‐Yuh , Tsai Mong‐Hsun , Lou Pei‐Jen 
Published: October 2022

Objective/HypothesisSpindle cell carcinoma of the head and neck (HNSpCC) is a rare variant of head and neck squamous cell carcinoma (HNSCC). This study evaluated the clinical characteristics and molecular signatures of such tumors.Study DesignRetrospective analysis.MethodsMedical records of patients diagnosed with HNSpCC from 1996 to 2018 were reviewed. The clinicopathologic features, treatment modalities, and survival status were carefully recorded. Whole exome sequencing (WES) was performed to evaluate the genetic signatures of HNSpCC.ResultsWe found that among all 71 patients included in this study, the majority of them were male, with tumors developing predominantly in the oral cavity. The 1‐, 3‐, and 5‐year disease‐specific survival (DSS) rates were 64.6%, 49.5%, and 43.9%, respectively. A high local recurrence (LR) and distant metastasis (DM) rate (47.9%–25.3%, respectively) were observed. A significant proportion (28.2%) of patients with the worst prognosis had history of previous head and neck cancer (HNC) and had been treated with radiotherapy (RT). WES revealed that those post‐RT SpCC shared common mutations with their previous HNC (pre‐RT SCC), but gained additional genetic traits, such as hypoxia and cell‐ECM interaction that were favorable for survival in an irradiated microenvironment. Distinct genetic landscapes in primary and post‐RT SpCC were also found.ConclusionsThis study demonstrates that HNSpCC is a unique entity with more aggressive behavior than conventional HNSCC. HNSpCC arising from a previously irradiated field is a predictor of dismal survival. Both genetic and microenvironmental factors contribute to this highly invasive tumor.Level of Evidence4 Laryngoscope, 133:2183–2191, 2023
Two temperate seagrass meadows are negligible sources of methane and nitrous oxide
Authors: Al‐Haj Alia N. , Chidsey Tyler , Fulweiler Robinson W. 
Published: October 2022

AbstractSeagrasses are globally important ecosystems that can help mitigate climate change by sequestering carbon (C). The net impact seagrass meadows have on the climate, however, also depends on methane (CH4) and nitrous oxide (N2O) fluxes. By not accounting for CH4 and N2O fluxes, we may be overestimating or underestimating the true C sequestration capacity of seagrasses. Yet, few observations of seagrass CH4 and N2O fluxes are available. Here, we quantified summer, dark/light CH4 and N2O fluxes across the sediment–water interface from seagrass meadows (Zostera marina) and adjacent nonvegetated sediments in two temperate bays with different environmental characteristics. On two occasions, we also estimated system wide air‐sea CH4 and N2O fluxes. We found the CH4 fluxes across the sediment–water interface varied between the two sites with one site emitting CH4 from vegetated sediments and a net zero flux at the other site. N2O fluxes across the sediment–water interface were not different from zero regardless of seagrass presence, although when we did measure a flux, there was more often a net uptake of N2O. We estimated that both systems were small net sources of CH4 and N2O to the atmosphere; however, the sediments are not likely the source of CH4 and N2O emitted to the atmosphere in the systems. Although the diffusive fluxes measured here are lower than those reported in the literature, they are consistent with our current understanding of seagrass sediments being variable sources of CH4 and potentially a negligible source or sink of N2O.
Glacier runoff influences biogeochemistry and resource availability in coastal temperate rainforest streams: Implications for juvenile salmon growth
Authors: Fellman Jason B. , Bellmore J. Ryan , Johnson Connor , Dunkle Matthew R. , Hood Eran 
Published: October 2022

AbstractMeltwater contributions to watersheds are shrinking as glaciers disappear, altering the flow, temperature, and biogeochemistry of freshwaters. A potential consequence of this landscape change is that streamflow patterns within glacierized watersheds will become more homogenous, potentially altering the capacity of watersheds to support Pacific salmon. To assess heterogeneity in stream habitat quality for juvenile salmon in a watershed in the Alaska Coast Mountains, we collected organic matter and invertebrate drift and measured streamwater physical and biogeochemical properties over the main runoff season in two adjacent tributaries, one fed mainly by rain and the other partially by glacier ice/snowmelt. We then used bioenergetic modeling to evaluate how temporal patterns in water temperature and invertebrate drift in each tributary influence juvenile salmon growth potential. Across the study period, average invertebrate drift concentrations were similar in non‐glacierized Montana (0.33 mg m−3) and glacier‐influenced McGinnis Creeks (0.38 mg m−3). However, seasonal patterns of invertebrate drift were temporally asynchronous between the two streams. Invertebrate drift and modeled fish growth were generally higher in McGinnis Creek in the spring and Montana Creek in the Summer. For juvenile salmon, tracking these resource asynchronies by moving between tributaries resulted in 20% greater growth than could be obtained within either stream alone. These results suggest that hydrologic heterogeneity within watersheds may enhance the diversity of foraging and growth opportunities for mobile aquatic organisms, which may be essential for supporting productive and resilient natural salmon runs.
Glucamines as green alternatives to conventional amino alcohols in metalworking fluids
Authors: Seddig Tom , Naundorf Tim , Kipphardt Helmut , Maison Wolfgang 
Published: October 2022

AbstractAmino alcohols like triethanolamine (TEA), 2‐amino‐2‐methylpropanol (AMP), or 1‐aminopropan‐2‐ol (MIPA) are common ingredients in industrial metalworking fluids. They are used in large quantities as alkaline additives for the neutralization of acidic corrosion inhibitors and are typical industrial products from the oil‐based value chain. The criteria for the selection of these additives have changed in the last decades and are nowadays not only performance‐driven but influenced by ecological considerations, toxicity, and regulatory standards. In this study, we evaluate the sugar derivative N,N‐diethylglucamine (DEGA) for use as a functional, nonvolatile, chemically stable, and cheap alkaline additive for common acidic corrosion inhibitors. Electrochemical analyses confirm anticorrosive properties of DEGA comparable to the gold standard TEA for iron and steel in an aqueous environment at a slightly alkaline pH. In addition, DEGA leads to extremely low levels of Co, Ni, and Cu leaching and can thus also serve as a nonvolatile substitute for currently used low‐leaching additives such as AMP and MIPA. The diethylamino‐motif is a sweet spot among different glucamine derivatives tested because it combines easy synthetic accessibility with good water solubility and low‐leaching properties. Besides this, DEGA can be prepared in one step from glucose and diethylamine, both derived from renewable resources.
Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison
Authors: Peixoto Tânia , Silva Daniel , Rodrigues Miguel , Neto Miguel , Silva Rui , Paiva Maria C. , Grenho Liliana , Fernandes Maria Helena , Lopes Maria A. 
Published: October 2022

AbstractSeveral polymers are researched for tendon repair as polyethylene terephthalate (PET) and polylactic acid (PLA). These are biocompatible and useful in scaffolding repair though with minimal success due to long‐term failure. There is a need to improve such scaffolds' design and physical–chemical nature. This work concerns surface functionalization of polymeric braids (PET and PLA) that fulfill the high mechanical demands of tissues such as tendons. The functionalization aims to incorporate amine groups in the braids' surface, improve cell adhesion, and consequently, the poor healing rate of these tissues and the biointegration of the braids. Two approaches are compared: the direct application of NH3 plasma and the surface grafting of EDA after O2 plasma activation. X‐ray photoelectron spectroscopy (XPS) shows that amine groups are effectively introduced onto the samples' surfaces. Besides, the plasma parameters chosen do not compromise the topography and tensile behavior of the braids. Resazurin assay and scanning electron microscopy show that the NH3 treatment improves cell–biomaterial interaction as improved cell adhesion and proliferation are observed. Both approaches are safe for biomedical applications. The NH3 plasma approach is more environmentally friendly, faster, and easier to scale‐up, showing potential for application in the final hybrid medical device.