bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacterial Stock Culture (Pathogenic and Non-Pathogenic) Isolated from Eucheuma Spinosum in Marine Microbiology Laboratory/ Stok Kultur Bakteri (Patogen dan Non-Patogen) Diisolasi dari Rumput Laut Eucheuma spinosum di Laboratorium Mikrobiologi Laut
Authors: Huyyirnah  , Syafri  
Published: August 2022

The diversity and unique characteristics of bacteria in marine ecosystems are a fascinating topic to explore and utilize extensively. These bacteria are also often used to test in practicum and research activities in the Marine Microbiology laboratory. This study aims to isolate and identify pathogenic and non-pathogenic bacteria of Eucheuma Spinosum used for bacterial stock culture in the Marine Microbiology Laboratory. The isolation was carried out using the pour-plate technique. Colony morphology of bacteria was observed visually such as their shape, edge, elevation, and color. Identification was verified using VITEK MS tool and then, additional testing was conducted to clarify pathogenicity. This study found eight different bacterial species isolated from E. Spinosum after the pathogenic testing, six pathogenic bacteria were found such as Vibrio alginolyticus, Shewanella algae, Staphylococcus kloosii, Staphylococcus gallinarum, Pseudomonas stutzeri, and Staphylococcus arlettae. While the nonpathogenic bacteria indicating that they do not describe clinical symptoms are Bacillus cereus and Brevibacterium casei.
Abstrak
Keragaman dan keunikan dari bakteri yang berasal dari ekosistem laut sangat menarik minat peneliti untuk terus dikaji dan dimanfaatkan. Penggunaan bakteri ini juga sering dibutuhkan dalam kegiatan praktikum dan penelitian di laboratorium mikrobiologi laut sebagai bakteri uji. Penelitian ini bertujuan mengisolasi dan mengidentifikasi bakteri patogen dan non-patogen dari rumput laut Eucheuma spinosum (E. spinosum) yang akan dijadikan sebagai stok kultur bakteri di laboratorium mikrobiologi laut. Metode isolasi menggunakan metode agar cawan tuang, pengamatan morfologi koloni dilakukan secara visual bentuk, tepian, elevasi dan warna koloni bakteri. Identifikasi menggunakan alat VITEK MS dan selanjutnya dilakukan pengujian patogenisitas. Penelitian ini menghasilkan delapan spesies bakteri berbeda yang berhasil dipisahkan dari rumput laut E. Spinosum, setelah uji patogenisitas diperoleh enam isolat yang bersifat patogen yaitu bakteri Vibrio alginolyticus, shewanella algae, Staphylococcus kloosii, Staphylococcus gallinarum, Pseudomonas stutzeri dan Staphylococcus arlettae. Sedangkan bakteri yang tidak bersifat patogen dalam yang menunjukkan tidak memberikan gejala klinis pada rumput laut adalah Bacillus cereus dan Brevibacterium casei.
Acromicric dysplasia due to a novel missense mutation in the fibrillin 1 gene in a three-generation family
Authors: Quitter Friederike , Flury Monika , Waldmueller Stephan , Schubert Tina , Koehler Katrin , Huebner Angela 
Published: August 2022

Abstract

Objectives
Short stature is one of the most common reasons for consulting a paediatric endocrinologist. Targeted diagnosis of familial short stature can be challenging due to a broad spectrum of differential diagnoses.

Case presentation
Here we report a novel mutation in the fibrillin 1 gene (FBN1) in six family members causing a mild phenotype of acromicric dysplasia. Additionally, we present the effects of growth hormone therapy in one of the affected children.

Conclusions
Acromicric dysplasia is a very rare skeletal dysplasia with a prevalence of <1 of 1.000.000 with only about 60 cases being reported worldwide. It is characterized by short stature, acromelia, mild facial dysmorphy but normal intelligence. This study aims to exemplify the clinical and molecular features of FBN1-related acromicric dysplasia and illustrates its pleiotropy by presenting a new, mild phenotype.
“I never get a thing that ain’t been used”: A diachronic corpus-based study of second-hand consumption
Authors: Gilquin Gaëtanelle 
Published: August 2022

Abstract
Second-hand consumption is often seen as a way of reducing one’s ecological footprint. In an attempt to find out how the representations of second-hand consumption in discourse have evolved over time, a corpus study of the word second-hand is carried out on the basis of the Corpus of Historical American English (COHA) (1820–2019). Frequency is considered, as well as collocates, which show the types of second-hand items that are mentioned in different time periods and which indicate how positively or negatively connoted second-hand consumption is. Together with a more qualitative approach, this analysis reveals variation in the frequency and connotations of the word second-hand, and some long-lasting stigma which survives for example through comparisons. The article ends with some suggestions as to how the image of second-hand consumption could be improved.
Geometry of conformal η-Ricci solitons and conformal η-Ricci almost solitons on paracontact geometry
Authors: Li Yanlin , Dey Santu , Pahan Sampa , Ali Akram 
Published: August 2022

Abstract
We prove that if an

η

\eta

-Einstein para-Kenmotsu manifold admits a conformal

η

\eta

-Ricci soliton then it is Einstein. Next, we proved that a para-Kenmotsu metric as a conformal

η

\eta

-Ricci soliton is Einstein if its potential vector field

V

V

is infinitesimal paracontact transformation or collinear with the Reeb vector field. Furthermore, we prove that if a para-Kenmotsu manifold admits a gradient conformal

η

\eta

-Ricci almost soliton and the Reeb vector field leaves the scalar curvature invariant then it is Einstein. We also construct an example of para-Kenmotsu manifold that admits conformal

η

\eta

-Ricci soliton and satisfy our results. We also have studied conformal

η

\eta

-Ricci soliton in three-dimensional para-cosymplectic manifolds.
Leptin signaling and leptin resistance
Authors: Liu Jiarui , Lai Futing , Hou Yujia , Zheng Ruimao 
Published: August 2022

Abstract
With the prevalence of obesity and associated comorbidities, studies aimed at revealing mechanisms that regulate energy homeostasis have gained increasing interest. In 1994, the cloning of leptin was a milestone in metabolic research. As an adipocytokine, leptin governs food intake and energy homeostasis through leptin receptors (LepR) in the brain. The failure of increased leptin levels to suppress feeding and elevate energy expenditure is referred to as leptin resistance, which encompasses complex pathophysiological processes. Within the brain, LepR-expressing neurons are distributed in hypothalamus and other brain areas, and each population of the LepR-expressing neurons may mediate particular aspects of leptin effects. In LepR-expressing neurons, the binding of leptin to LepR initiates multiple signaling cascades including janus kinase (JAK)–signal transducers and activators of transcription (STAT) phosphatidylinositol 3-kinase (PI3K)-protein kinase B (AKT), extracellular regulated protein kinase (ERK), and AMP-activated protein kinase (AMPK) signaling, etc., mediating leptin actions. These findings place leptin at the intersection of metabolic and neuroendocrine regulations, and render leptin a key target for treating obesity and associated comorbidities. This review highlights the main discoveries that shaped the field of leptin for better understanding of the mechanism governing metabolic homeostasis, and guides the development of safe and effective interventions to treat obesity and associated diseases.
Tunable optical nonlinearity of indium tin oxide for optical switching in epsilon-near-zero region
Authors: Lau Kuen Yao , Yang Yuting , Zhao Di , Liu Xiaofeng , Qiu Jianrong 
Published: August 2022

Abstract
The propagation of light in the epsilon-near-zero (ENZ) region of materials exhibits intriguing linear and nonlinear optical phenomenon that have been extensively exploited for a plethora of applications. Here, we show that the optical properties as well as the ENZ wavelength of magnetron-sputtered indium tin oxide (ITO) thin films could be judiciously engineered. The measurement of nonlinear optical properties reveals that the control of deposition conditions allows for the tuning of absorptive optical nonlinearity between saturable absorption and reverse saturable absorption. The ENZ wavelength for the ITO film is deduced as around 1553 nm. We obtain the highest third-order nonlinear absorption coefficient and imaginary part of third-order nonlinear susceptibility for the ITO thin film through Z-scan method as −50.56 cm/GW and ∼38 × 10−14 e.s.u. at 1050 nm, and −64.50 cm/GW and ∼45 × 10−14 e.s.u. at 1550 nm, respectively. We demonstrate further that the strong saturable absorption of the ITO thin film enables Q-switched pulse laser generation in ∼1050 and ∼1550 nm regions with tunable repetition rates and pulse energies. The present results suggest the great application potential of the ITO thin film in the field of nonlinear optical devices.
Investigation of CDH1 germline mutations in Turkish patients with Kaposi’s sarcoma
Authors: Celik Betul , Tuncer Seref Bugra 
Published: August 2022

Abstract

Objectives
Kaposi’s sarcoma (KS) develops from the lining cells of blood or lymphatic vessels and may appear as red, purple, brown, or black lesions. E-cadherin, CDH1, is a cell adhesion molecule located on the surface of epithelial cells. CDH1 gene expression is downregulated in several cancers and is considered a tumor suppressor gene involved in epithelial-mesenchymal transition in carcinomas. Loss of CDH1 gene expression is observed in many carcinomas, mainly diffuse gastric carcinomas and lobular breast carcinomas, as well as skin tumors. This study investigates the CDH1 germline mutations in HIV-negative (Human Immunodeficiency) Virus KS patients in the Turkish population.

Methods
The study examined 25 peripheral blood mononuclear cells from KS patients using the Sanger sequencing technique.

Results
Sixteen exons of the CDH1 gene were sequenced, and a pathogenic functional germline mutation, HET c.2245C > T, p.(Arg749Trp) rs776975632, NM _004360.5, was identified in a patient with a family history of gastric and breast cancer with a high number of lesions compared to other KS patients. Discussion: KS patients with a family history of cancer could be screened for CDH1 gene and cancer-related-gen variants in the future.

Conclusions
KS is a rare malignancy, and genetic analysis will benefit KS patients. Further studies are needed to describe better the variations detected in a large number of KS patients in this study.
Knappes Gas – Optionen zur Verringerung der Deckungslücke in Deutschland
Authors: Holz Franziska , Kemfert Claudia , Sogalla Robin 
Published: August 2022

Abstract
In this article, we provide the results of a simple quantitative analysis of the potential remedies to reduce the supply gap in Germany that arises if Russia stops delivering natural gas. We take into account the supply potential of other suppliers, and combine this with an analysis of the additional supply potential by stopping electricity generation from natural gas. Moreover, we investigate the effect of minimum storage requirements. We find that a realistic expansion of supply by other providers, in particular Norway, combined with the temporary replacement of gas-fired power plants by coal-fired power plants and expansive storage filling to 90 % of storage capacity strongly reduces the winter supply gap. In summer months, the supply gap is even negative, indicating the potential to store even more for the winter. In other words, most of the natural gas consumption can be maintained even if Russia fails to supply to Germany. It is important that the temporary replacement of gas-fired power plants by coal-fired power plants is not accompanied by an expansion of the CO2 emission cap, in order to ensure emission neutrality of the measure. If the expansion of renewable generation capacities proceeds as planned in the German government’s „Easter Package“ of spring 2022, the increased use of coal-fired power plants can be temporary and the coal exit in 2030 can be maintained.
Modelling prevalent cardiovascular disease in an urban Indigenous population
Authors: Avery Lisa , Maddox Raglan , Abtan Robert , Wong Octavia , Rotondi Nooshin Khobzi , McConkey Stephanie , Bourgeois Cheryllee , McKnight Constance , Wolfe Sara , Flicker Sarah , Macpherson Alison , Smylie Janet , Rotondi Michael 
Published: August 2022

Abstract
Objective
Studies have highlighted the inequities between the Indigenous and non-Indigenous populations with respect to the burden of cardiovascular disease and prevalence of predisposing risks resulting from historical and ongoing impacts of colonization. The objective of this study was to investigate factors associated with cardiovascular disease (CVD) within and specific to the Indigenous peoples living in Toronto, Ontario, and to evaluate the reliability and validity of the resulting model in a similar population.

Methods
The Our Health Counts Toronto study measured the baseline health of Indigenous community members living in Toronto, Canada, using respondent-driven sampling. An iterative approach, valuing information from the literature, clinical insight and Indigenous lived experiences, as well as statistical measures was used to evaluate candidate predictors of CVD (self-reported experience of discrimination, ethnic identity, health conditions, income, education, age, gender and body size) prior to multivariable modelling. The resulting model was then validated using a distinct, geographically similar sample of Indigenous people living in Hamilton, Ontario, Canada.

Results
The multivariable model of risk factors associated with prevalent CVD included age, diabetes, hypertension, body mass index and exposure to discrimination. The combined presence of diabetes and hypertension was associated with a greater risk of CVD relative to those with either condition and was the strongest predictor of CVD. Those who reported previous experiences of discrimination were also more likely to have CVD. Further study is needed to determine the effect of body size on risk of CVD in the urban Indigenous population. The final model had good discriminative ability and adequate calibration when applied to the Hamilton sample.

Conclusion
Our modelling identified hypertension, diabetes and exposure to discrimination as factors associated with cardiovascular disease. Discrimination is a modifiable exposure that must be addressed to improve cardiovascular health among Indigenous populations.
Doença de Krabbe: relato de casos sobre o espectro fenotípico de uma leucodistrofia metabólica multifacetada
Authors: Machado Graziele Santos , Barbosa Gabriela Cruz , Lourenço Charles , Carneiro Zumira 
Published: August 2022

Introdução: A doença de Krabbe, também conhecida como leucodistrofia de células globoides, é uma enfermidade autossômica recessiva rara causada pela deficiência da galactocerebrosidase (GALC). Seu diagnóstico baseia-se em teste enzimático seguido por estudo genético-molecular do gene GALC. As manifestações clínicas são diversas e incluem alterações motoras, envolvimento cognitivo com irritabilidade, espasticidade e regressão do desenvolvimento, com óbito em idade bastante precoce na forma infantil clássica. Nas formas de início tardio, os fenótipos são mais atenuados com anormalidades da marcha e progressão mais lenta. Objetivos: Demonstrar a variabilidade fenotípica de uma coorte brasileira considerando o caráter multiétnico dessa população e enfatizar a importância do diagnóstico precoce para possível tratamento; uma vez que há evidências de melhora da sua evolução e prognóstico. Relato de Casos: São apresentados três casos de pacientes do sexo masculino com Leucodistrofia de Células Globoides; todos filhos de pais não consanguíneos, com diagnóstico molecular e sem variação comum, reforçando o caráter multiétnico da população brasileira. A idade de início das manifestações clínicas foi variável (um mês, oito meses e dois anos) e o diagnóstico da doença foi realizado entre os três meses e os dois anos de vida. Os pacientes apresentaram atraso no desenvolvimento neuropsicomotor, diversas manifestações neurológicas e a ressonância magnética de encéfalo foi similar entre eles. Os pacientes do estudo não receberam o tratamento, pois não foram diagnosticados precocemente; dois deles apresentaram complicações da doença e evoluíram a óbito. Conclusão: A Leucodistrofia de Células Globoides apresenta uma alta variabilidade fenotípica com múltiplas manifestações. Além disso, o diagnóstico precoce da doença é um desafio que impacta diretamente no prognóstico e na única terapia disponível no momento; o transplante de medula óssea. Adicionalmente, o diagnóstico acurado da enfermidade viabiliza o aconselhamento genético familiar e auxilia na melhora dos cuidados de reabilitação do paciente.
Digital Literacy to Improve Pedagogical and Professional Competence of Early Childhood Teacher
Authors: Khotimah Nurul , Reza Muhammad 
Published: August 2022

Abstrak: Pada era transisi revolusi industri 4.0 menjadi 5,0, guru memerlukan kemampuan literasi komputer dengan kemampuan untuk mencari dan mengelola data secara digital serta mengoperasikan komputer. Para guru kemudian mengikuti Pendidikan Profesi Guru (PPG) dalam jabatan yang difasilitasi aplikasi google suite untuk guru yang profesional pada era revolusi industri 5.0. Penelitian ini bertujuan untuk mengimplementasikan kemampuan digital pada guru Pendidikan Anak Usia Dini (PAUD) dalam pembelajaran PPG PAUD dan meningkatkan kemampuan pedagogi guru PAUD. Metode yang digunakan adalah mixed method dengan pengumpulan data melalui google form, hasil wawancara, studi literatur, dan relevansi data melalui penelitian yang relevan. Hasil menunjukkan kenaikan yang signifikan pada kemampuan profesional guru PPG dalam jabatan tahap 1. Guru mendapatkan pendalaman materi pedagogi dan profesional, pengembangan perangkat pembelajaran, review perangkat pembelajaran, dan tes komprehensif.Abstract: In the transition era of the industrial revolution 4.0 to 5.0, teachers need computer literacy skills with the ability to search and manage data digitally and operate computers. On these grounds, numerous teachers take part in the teacher professional development program for in-service teachers (PPG dalam Jabatan) facilitated by the Google Suite application for professional teachers in the 5.0 industrial revolution era. This study aims to implement digital skills for early childhood education (PAUD) teachers in PPG and improve the pedagogical abilities of PAUD teachers. The method used is a mixed methods with data collection using Google Forms, interviews, literature studies, and data relevance through relevant research. The results show a significant increase in the professional ability of teachers who join stage 1 of the program. Teachers deepen the pedagogical and professional materials, development of educational materials, examination of learning material, and comprehensive tests.
Transformation of Islamic education curriculum development policy in the national education system
Authors: Akrim A. , Setiawan Hasrian Rudi , Selamat Selamat , Ginting Nurman 
Published: August 2022

The most essential tool in any education system and for any learning process is the educational curriculum. Curriculum orientation is used as a reference to the direction of studies in learning outcomes. In the presence of western-based models in the development of educational techniques, students in Islamic universities are found to be aloof from their religious and cultural norms as they graduate from educational institutions. This paper aims to describe the transformation of Islamic Education in national education programs and curricula. For the purpose of this study, we have taken Islamic pedagogy and research methodology as well as cultural reinvigoration as the independent variable. Whereas the development of national education system along the Islamic model is the dependent variable. State organized curriculum is deemed as the mediator, which affects the later and is affected by the presence of former. Data was collected via questionnaires from a total of 550 respondents where the respondents chosen were experts in curriculum development and Islamic education. The data collected was analyzed using the SPSS and Smart PLS. The results illustrate that with cultural reinvigoration and development of Islamic pedagogy, not only the development of the curriculum will be facilitated in Indonesia, but its implementation will also be expedited.
Keywords: Curriculum, Islamic, education, pedagogy, culture, national
Assertiveness as the predictor of university students’ academic achievement
Authors: Kalzhanova Anar , Kalzhanova Dana , Murzina Saule , Nurmukhanbetova Nurgul , Naviy Liza , Yermentayeva Ardakh 
Published: August 2022

The purpose of this research is to evaluate the relationship between the academic success of university students and their assertiveness levels with student opinions. Descriptive analysis, one of the qualitative research methods, was used in the research. The study group of the research consists of 40 university students studying at various universities in Kazakhstan in the 2021–2022 academic year. The data of the study were collected with a semi-structured interview form developed by the researchers. As a result of the research, the vast majority of university students stated that their academic achievement and assertiveness levels are at a moderate level. In addition, the majority of university students participating in the research stated that there is a significant relationship between the level of assertiveness and the level of academic achievement. The majority of students stated that as the level of assertiveness increases, academic success will increase. In line with the results obtained from the research, it has become necessary to add courses related to assertiveness to the curriculum of the education given at universities to improve the assertiveness levels of university students.
Keywords: Assertiveness, academic achievement, university students;