bluuebunny/crossref_metadata_2025_split huggingface dataset:
Testing the association of positive and negative extended contact with intergroup contact intentions in China: The mediating role of intergroup anger, empathy, and happiness
Authors: Wang Changcheng , Cocco Veronica Margherita , Vezzali Loris 
Published: February 2022

AbstractA large corpus of research has investigated extended contact and its psychological mechanisms. However, research taking into account the valence of extended contact, as well as the psychological mechanisms underlying the effects of negative extended contact, is lacking. We examined the associations of Chinese people with rural origins’ (N = 1287) positive and negative extended contact with contact intentions toward Chinese people with urban origins. Three affective variables (empathy, happiness, and anger) were tested as mediators. Results revealed that the associations of positive and negative extended contact with contact intentions were mediated by empathy, happiness, and anger. This research highlights the importance of examining both positive and negative extended contact as well as affective variables as extended contact mediators.
MicroRNA‐495‐3p diminishes doxorubicin‐induced cardiotoxicity through activating AKT
Authors: Meng Jun , Xu Can 
Published: February 2022

AbstractDoxorubicin (Dox) is a broad‐spectrum antitumour agent; however, its clinical application is impeded due to the cumulative cardiotoxicity. The present study aims to investigate the role and underlying mechanisms of microRNA‐495‐3p (miR‐495‐3p) in Dox‐induced cardiotoxicity. Herein, we found that cardiac miR‐495‐3p expression was significantly decreased in Dox‐treated hearts, and that the miR‐495‐3p agomir could prevent oxidative stress, cell apoptosis, cardiac mass loss, fibrosis and cardiac dysfunction upon Dox stimulation. In contrast, the miR‐495‐3p antagomir dramatically aggravated Dox‐induced cardiotoxicity in mice. Besides, we found that the miR‐495‐3p agomir attenuated, while the miR‐495‐3p antagomir exacerbated Dox‐induced oxidative stress and cellular injury in vitro. Mechanistically, we demonstrated that miR‐495‐3p directly bound to the 3′‐untranslational region of phosphate and tension homology deleted on chromosome ten (PTEN), downregulated PTEN expression and subsequently activated protein kinase B (PKB/AKT) pathway, and that PTEN overexpression or AKT inhibition completely abolished the cardioprotective effects of the miR‐495‐3p agomir. Our study for the first time identify miR‐495‐3p as an endogenous protectant against Dox‐induced cardiotoxicity through activating AKT pathway in vivo and in vitro.
Chronic alcohol exposure induces hepatocyte damage by inducing oxidative stress, SATB2 and stem cell‐like characteristics, and activating lipogenesis
Authors: Yu Wei , Ma Yiming , Shrivastava Sushant K. , Srivastava Rakesh K. , Shankar Sharmila 
Published: February 2022

AbstractAlcohol is a risk factor for hepatocellular carcinoma (HCC). However, the molecular mechanism by which chronic alcohol consumption contributes to HCC is not well understood. The purpose of the study was to demonstrate the effects of chronic ethanol exposure on the damage of human normal hepatocytes. Our data showed that chronic exposure of hepatocytes with ethanol induced changes similar to transformed hepatocytes that is, exhibited colonies and anchorage‐independent growth. These damaged hepatocytes contained high levels of reactive oxygen species (ROS) and showed induction of the SATB2 gene. Furthermore, damaged hepatocytes gained the phenotypes of CSCs which expressed stem cell markers (CD133, CD44, CD90, EpCAM, AFP and LGR5), and pluripotency maintaining factors (Sox‐2, POU5F1/Oct4 and KLF‐4). Ethanol exposure also induced Nanog, a pluripotency maintaining transcription factor that functions in concert with Oct4 and SOX‐2. Furthermore, ethanol induced expression of EMT‐related transcription factors (Snail, Slug and Zeb1), N‐Cadherin, and inhibited E‐cadherin expression in damaged hepatocytes. Ethanol enhanced recruitment of SATB2 to promoters of Bcl‐2, Nanog, c‐Myc, Klf4 and Oct4. Ethanol also induced activation of the Wnt/TCF‐LEF1 pathway and its targets (Bcl‐2, Cyclin D1, AXIN2 and Myc). Finally, ethanol induced hepatocellular steatosis, SREBP1 transcription, and modulated the expression of SREBP1c, ACAC, ACLY, FASN, IL‐1β, IL‐6, TNF‐α, GPC3, FLNB and p53. These data suggest that chronic alcohol consumption may contribute towards the development of HCC by damaging normal hepatocytes with the generation of inflammatory environment, induction of SATB2, stem cell‐like characteristics, and cellular steatosis.
Genetic analysis of potential biomarkers and therapeutic targets in ferroptosis from coronary artery disease
Authors: Wu Xun , Qin Kele , Iroegbu Chukwuemeka Daniel , Xiang Kun , Peng Jun , Guo Jianjun , Yang Jinfu , Fan Chengming 
Published: February 2022

AbstractFerroptosis plays a key role in the death of cells including cardiomyocytes, and it is related to a variety of cardiac diseases. However, the role of ferroptosis‐related genes (FRGs) in coronary artery disease (CAD) is not well characterized. We downloaded CAD‐related information and FRGs from the gene expression omnibus (GEO) database and Ferroptosis Database (FerrDb) respectively. A total of 10 CAD‐related DE‐FRGs were obtained, which were closely linked to autophagy regulation and immune response. Subsequently, CA9, CBS, CEBPG, HSPB1, SLC1A4, STMN1 and TRIB3 among the 10 DE‐FRGs were identified as marker genes by LASSO and SVM‐RFE algorithms, which had tolerable diagnostic capabilities. Subsequent functional enrichment analysis showed that these marker genes may play a corresponding role in CAD by participating in the regulation of immune response, amino acid metabolism, cell cycle and multiple pathways related to the pathogenesis of CAD. Furthermore, a total of 58 drugs targeting 7 marker genes had been obtained. On the contrary, the ceRNA network revealed a complex regulatory relationship based on the marker genes. Also, CIBERSORT analysis showed that the changes in the immune microenvironment of CAD patients may be related to CBS, HSPB1 and CEBPG. We developed a diagnostic potency and provided an insight for exploring the mechanism for CAD. Before clinical application, further research is needed to test its diagnostic value for CAD.
LRRK2‐G2019S mice display alterations in glutamatergic synaptic transmission in midbrain dopamine neurons
Authors: Skiteva Olga , Yao Ning , Sitzia Giacomo , Chergui Karima 
Published: February 2022

AbstractThe progressive degeneration of dopamine (DA) neurons in the substantia nigra compacta (SNc) leads to the emergence of motor symptoms in patients with Parkinson's disease (PD). To propose neuroprotective therapies able to slow or halt the progression of the disease, it is necessary to identify cellular alterations that occur before DA neurons degenerate and before the onset of the motor symptoms that characterize PD. Using electrophysiological, histochemical, and biochemical approaches, we have examined if glutamatergic synaptic transmission in DA neurons in the SNc and in the adjacent ventral tegmental area (VTA) was altered in middle‐aged (10–12 months old) mice with the hG2019S point mutation (G2019S) in the leucine‐rich repeat kinase 2 (LRRK2) gene. G2019S mice showed increased locomotion and exploratory behavior compared with wildtype (WT) littermates, and intact DA neuron integrity. The intrinsic membrane properties and action potential characteristics of DA neurons recorded in brain slices were similar in WT and G2019S mice. Initial glutamate release probability onto SNc‐DA neurons, but not VTA‐DA neurons, was reduced in G2019S mice. We also found reduced protein amounts of the presynaptic marker of glutamatergic terminals, VGLUT1, and of the GluA1 and GluN1 subunits of AMPA and NMDA receptors, respectively, in the ventral midbrain of G2019S mice. These results identify alterations in glutamatergic synaptic transmission in DA neurons of the SNc and VTA before the onset of motor impairments in the LRRK2‐G2019S mouse model of PD.
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Up‐regulation of miR‐335 and miR‐674‐3p in the rostral ventrolateral medulla contributes to stress‐induced hypertension
Authors: Zhang Shuai , Xing Mengyu , Chen Gaojun , Tong Lei , Zhang Haili , Du Dongshu 
Published: February 2022

AbstractThe rostral ventrolateral medulla (RVLM) is known as the vasomotor center that plays a crucial role in mediating the development of stress‐induced hypertension (SIH). MicroRNAs (miRNAs) are involved in many different biological processes and diseases. However, studies that evaluated the roles of miRNAs in the RVLM during SIH do not exist. Here, we performed RNA sequencing to explore the genome‐wide miRNA profiles in RVLM in an SIH rat model established by administering electric foot‐shocks and noises. The function of miRNAs in blood pressure regulation was determined in vivo via the intra‐RVLM microinjection of the agomir or antagomir. Furthermore, the underlying mechanisms of miRNAs on SIH were investigated through in vitro and in vivo experiments, like gain‐of‐function. We discovered 786 miRNA transcripts among which 4 were differentially expressed. The over‐expression of miR‐335 and miR‐674‐3p in RVLM dramatically increased the heart rate (HR), arterial blood pressure (ABP), systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP) levels of normotensive rats, whereas the knockdown of miR‐335 and miR‐674‐3p in RVLM markedly reduced the HR, ABP, SBP, DBP, and MAP levels of SIH rats. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation revealed that miR‐335 and miR‐674‐3p participated in regulating the development of SIH from different aspects, like apoptosis‐multiple species pathway. Sphk1, whose expression was markedly decreased in SIH, was identified as a novel target of miR‐335. MiR‐335 over‐expression substantially reduced the expression of Sphk1 and promoted neural apoptosis, and its inhibition had opposite effects. Re‐introduction of Sphk1 dramatically abrogated the apoptosis induced by miR‐335. This study provides the first systematic dissection of the RVLM miRNA landscape in SIH. MiR‐335 and miR‐674‐3p act as SIH promoters, and the identified miR‐335/Sphk1/apoptosis axis represents one of the possible mechanisms. These miRNAs can be exploited as potential targets for the molecular‐based therapy of SIH.

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Income and geographically constrained generosity
Authors: Zhao Yujie , Zhou Xinyue 
Published: February 2022

AbstractHow to target individuals for charitable behavior to distant others is a major challenge. This paper tests the possibility that individuals with higher income engage in more charitable behavior toward distant beneficiaries relative to individuals with lower income. A multi‐method approach offers evidence for this prediction. Survey data (N = 2957) demonstrates that income is positively related to allocations of money to distant beneficiaries (foreign medical staff). Richer (vs. poorer) individuals are also more likely to volunteer to assist distant beneficiaries (Study 2, N = 397; Study 3, N = 250). Moreover, we tested this prediction with data from 66,669 respondents across 49 countries and found that those with higher income have higher global identity, which, in turn, leads to proffering more help toward distant others (Study 4).
Characterizing the time course of cerebrovascular reactivity in multiple sclerosis
Authors: Lincoln John A. , Hasan Khader M. , Gabr Refaat E. , Wolinsky Jerry S. 
Published: February 2022

AbstractBackground and PurposeChanges in cerebral perfusion occur early in relapsing and progressive multiple sclerosis (MS) patients, though whether cerebral blood flow (CBF) can be altered by therapy is unknown. We sought to characterize the time course of change in CBF (cerebral vascular reactivity [CVR]), following intravenous (IV) acetazolamide (ACZ) in whole brain and within various gray and white matter brain regions in MS patients.MethodsWe enrolled five relapsing MS patients on injectable therapies. Participants received a 1000 mg IV bolus of ACZ and CBF was measured using pseudocontinuous arterial spin labeling MRI. To quantify differences in time course between patients, we calculated the numerical integration of CVR over time using the trapezoidal rule to estimate area under the curve (AUC(CVR)).ResultsA change in whole brain CBF of 30%‐65% was seen in all participants at 15 minutes after ACZ challenge. CBF increases >20% above baseline were sustained for 90 minutes within whole‐brain, normal‐appearing white matter and total T2‐hyperintense lesioned tissue. AUC(CVR) values for both gray (cortical and deep gray matter) and white (normal‐appearing and T2‐lesioned) matter regions were similar between patients.ConclusionOur findings show a prolonged time course in vascular reactivity after ACZ stimulus in MS patients with a similar time course for both gray and white matter brain regions, including in previously injured tissue. Our preliminary results suggest that blood flow can be augmented in the established MS lesion suggesting that even previously injured tissue might be responsive to treatment.
Oral motor function in obesity
Authors: Castro Mariana Cristina Zanin , Santos Carla Manfredi dos , Lucas Raquel Eduardo , de Felício Claudia Maria , Dantas Roberto Oliveira 
Published: February 2022

AbstractBackgroundObese individuals may have impaired oral sensory functioning and abnormal oral motor function, a consequence of fat deposition in muscles.ObjectiveTo evaluate the oral motor function in obese individuals.Material and MethodsThree observational cross‐sectional studies were performed. In total, 140 participants were evaluated: (1) orofacial myofunctional evaluation (OMES) was performed in 26 obese and 26 control subjects; (2) time taken for suction of 50 ml of water through straws of 3 mm and 6 mm of diameter was measured in 30 obese and 30 control subjects; (3) the oral phase of swallowing of 5 ml moderately thick and 5 ml extremely thick boluses was analysed by videofluoroscopy in 14 obese and 14 control subjects. Obese and non‐obese control subjects had body mass index ≥40 kg/m2and <30 kg/m2, respectively.ResultsObese subjects had worse oral myofunctional evaluation scores in posture/appearance (lips, jaw, cheeks, tongue and hard palate), in mobility (lips, tongue, jaw and cheeks) and in breathing, deglutition and mastication functions (p ≤ .020). The OMES total score was 73.5 ± 5.5 in obese and 92.8 ± 3.7 in controls subjects (p < .001). There was no difference between the groups in the time taken for 50 ml of water suction through the 3‐mm‐ or 6‐mm‐diameter straw. Videofluoroscopic evaluation of the bolus swallowed demonstrated a longer oral preparation time in obese individuals for both boluses (p ≤ .040) and no difference in oral transit time (p ≥ .140).ConclusionA moderate change in oral motor function was observed in obese individuals with BMI ≥40 kg/m2.
Beliefs about the nature of knowledge shape responses to the pandemic: Epistemic beliefs, the Dark Factor of Personality, and COVID‐19–related conspiracy ideation and behavior
Authors: Rudloff Jan Philipp , Hutmacher Fabian , Appel Markus 
Published: February 2022

AbstractObjectiveGlobal challenges such as climate change or the COVID‐19 pandemic have drawn public attention to conspiracy theories and citizens' non‐compliance to science‐based behavioral guidelines. We focus on individuals' worldviews about how one can and should construct reality (epistemic beliefs) to explain the endorsement of conspiracy theories and behavior during the COVID‐19 pandemic and propose the Dark Factor of Personality (D) as an antecedent of post‐truth epistemic beliefs.Method and ResultsThis model is tested in four pre‐registered studies. In Study 1 (N = 321), we found first evidence for a positive association between D and post‐truth epistemic beliefs (Faith in Intuition for Facts, Need for Evidence, Truth is Political). In Study 2 (N = 453), we tested the model proper by further showing that post‐truth epistemic beliefs predict the endorsement of COVID‐19 conspiracies and disregarding COVID‐19 behavioral guidelines. Study 3 (N = 923) largely replicated these results at a later stage of the pandemic. Finally, in Study 4 (N = 513), we replicated the results in a German sample, corroborating their cross‐cultural validity. Interactions with political orientation were observed.ConclusionOur research highlights that epistemic beliefs need to be taken into account when addressing major challenges to humankind.
Integrating hepatitis C virus screening of baby boomers at a community hospital emergency department
Authors: Wu Gregory , Zhou Aiqi , Kwon Steve 
Published: February 2022

AbstractApproximately 2.4 million Americans are infected with hepatitis C virus (HCV), and persons born from 1945 through 1965 (i.e. baby boomers) account for nearly three‐fourths of all HCV infections. The purpose of this study was to implement HCV screening for baby boomers presenting to a community hospital emergency department (ED) and to facilitate linkage to care. We developed a process within our electronic medical record system to screen patients for HCV testing eligibility, link eligible patients to laboratory orders, notify patients of HCV test results (via patient navigator) and track follow‐up care. We tracked performance from February 2016 to December 2018. Sociodemographic compositions and linkage to care rates of all participants were evaluated. A total of 14,927 patients from the birth cohort of 1945–1965 were screened for HCV. Of those tested, 555 (3.7%) had a positive HCV antibody test and 147 were HCV RNA‐positive patients (1.0%) demonstrating that only 27% of HCV antibody‐positive individuals were chronically infected. Males, black race and USA‐born baby boomers had a higher prevalence of HCV antibody and viral load positivity (p < 0.05). Initially, only 17.6% of patients were ultimately linked to care, which improved to over 94% after the implementation of patient navigation support. There is a need for HCV screening protocol in the community. The cost of implementing an HCV screening programme must include information technology and a team of care coordinators to improve screening rates and facilitate linkage to continual care using the four pillars framework.
Recombinant human thrombopoietin increases platelet count in severe thrombocytopenic patients with hepatitis B‐related cirrhosis: Multicentre real‐world observational study
Authors: Feng Ru , Liu Yi , Zhu Xiao‐Lu , Zhai Wan‐Yi , He Yun , Fu Hai‐Xia , Jiang Qian , Jiang Hao , Lu Jin , Liu Hui , Wang Jing‐Wen , Wang Hao , Xie Yan‐Di , Ma Hui , Huang Xiao‐Jun , Zhang Xiao‐Hui 
Published: February 2022

AbstractPatients with hepatitis B‐related cirrhosis complicated with thrombocytopenia have a higher risk of bleeding, which may lead to higher mortality. We aimed to explore the efficacy and safety of recombinant human thrombopoietin (rhTPO) in the treatment of hepatitis B‐related cirrhosis complicated with severe thrombocytopenia. Patients with hepatitis B‐related compensated liver cirrhosis complicated with severe thrombocytopenia were divided into four groups according to the treatment method for thrombocytopenia. Platelet counts, the appearance of bleeding symptoms and adverse events were evaluated during the observation period. Also during the observational period, the platelet counts in the prednisone group, rhTPO group and prednisone plus rhTPO group were higher than those in the no treatment group. Patients without splenomegaly reacted better to rhTPO. Fewer bleeding events of grade 2 or worse were observed in the three treatment groups compared to the no treatment group. The platelet counts at baseline and treatment with rhTPO and/or prednisone were factors associated with bleeding events of grade 2 or worse in multivariate analysis. There could be a potential advantage for the use of rhTPO plus prednisone based on higher platelet counts and fewer bleeding events. Treatment with rhTPO was more effective in patients without splenomegaly.
The biliary microbiome in ischaemic‐type biliary lesions can be shaped by stenting but is resilient to antibiotic treatment
Authors: Klein Friederike , Wellhöner Freya , Plumeier Iris , Kahl Silke , Chhatwal Patrick , Vital Marius , Voigtländer Torsten , Pieper Dietmar H. , Manns Michael P. , Lenzen Henrike , Solbach Philipp , Heidrich Benjamin 
Published: February 2022

AbstractThis study aims to characterize the biliary microbiome as neglected factor in patients with ischaemic‐type biliary lesions (ITBL) after liver transplantation. Therefore, the V1–V2 region of the 16S rRNA gene was sequenced in 175 bile samples. Samples from patients with anastomotic strictures (AS) served as controls. Multivariate analysis and in silico metagenomics were applied cross‐sectionally and longitudinally. The microbial community differed significantly between ITBL and AS in terms of alpha and beta diversity. Both, antibiotic treatment and stenting were associated independently with differences in the microbial community structure. In contrast to AS, in ITBL stenting was associated with pronounced differences in the biliary microbiome, whereas no differences associated with antibiotic treatment could be observed in ITBL contrasting the pronounced differences found in AS. Bacterial pathways involved in the production of antibacterial metabolites were increased in ITBL with antibiotic treatment. After liver transplantation, the biliary tract harbours a complex microbial community with significant differences between ITBL and AS. Fundamental changes in the microbial community in ITBL can be achieved with biliary stenting. However, the effect of antibiotic treatment in ITBL was minimal. Therefore, antibiotics should be administered wisely in order to reduce emerging resistance of the biliary microbiome towards external antibiotics.
Adverse effects of Diptera flies on northern ungulates: Rangifer, Alces, and Bison
Authors: Benedict Bridgett M. , Barboza Perry S. 
Published: February 2022

Abstract

Flies (Diptera) damage ungulates far beyond the injury of their bite wounds: they are vectors of diseases and cause ungulates to lose foraging opportunities due to avoidance behaviour. We can use the behavioural and physiological responses of bison Bison spp. (Artiodactyla: Bovidae), caribou/reindeer Rangifer tarandus (Artiodactyla: Cervidae), and moose/elk Alces alces (Artiodactyla: Cervidae) to assess the impacts of flies on these ungulates.

Ungulates rely on morphological and physiological resistance to flies at low intensities of exposure. However, as fly exposure increases, ungulates begin to react with behavioural avoidance in addition to increasing their physiological response. Rangifer tarandus are highly sensitive to flies and respond quickly to their presence by avoidance behaviours that incur fitness costs through reduced body mass. Alces alces are less reactive to fly exposure, enduring the presence of flies and maintaining a low loss of fitness, sometimes dying from the cumulative effects of exposure. Bison spp. may use a facultative strategy that depends upon the prevalence of flies and associated diseases in their environment. Among these strategies, variables such as the type of fly bite, presence and degree of infection, and heritability of resistance affect individual host survival.

Relationships between flies and ungulates can integrate multiple scales of organisation in the ecosystem to reflect system stability. Climate change is predicted to alter the species composition and seasonal phenology of flies and the associated effects of wounding and vector‐borne disease on ungulate populations that are central to the functions of Arctic and temperate ecosystems in the Northern Hemisphere.