bluuebunny/crossref_metadata_2025_split huggingface dataset:
Restricted truncal sagittal movements of rapid eye movement behaviour disorder
Authors: Wasserman Danielle , Gullone Silvia , Duncan Iain , Veronese Mattia , Gnoni Valentina , Higgins Sean , Birdseye Adam , Gelegen Emine Cigdem , Goadsby Peter J. , Ashkan Keyoumars , Ray Chaudhuri K. , Tononi Giulio , Drakatos Panagis , Rosenzweig Ivana 
Published: March 2022

AbstractUnlike sleep-walkers, patients with rapid-eye-movement-behaviour disorder (RBD) rarely leave the bed during the re-enactment of their dreams. RBD movements may be independent of spatial co-ordinates of the ‘outside-world’, and instead rely on (allocentric) brain-generated virtual space-maps, as evident by patients’ limited truncal/axial movements. To confirm this, a semiology analysis of video-polysomnography records of 38 RBD patients was undertaken and paradoxically restricted truncal/thoraco-lumbar movements during complex dream re-enactments demonstrated.
Gasdermin E mediates resistance of pancreatic adenocarcinoma to enzymatic digestion through a YBX1–mucin pathway
Authors: Lv Jiadi , Liu Yuying , Mo Siqi , Zhou Yabo , Chen Fengye , Cheng Feiran , Li Cong , Saimi Dilizhatai , Liu Mengyu , Zhang Huafeng , Tang Ke , Ma Jingwei , Wang Zhenfeng , Zhu Qiangqiang , Tong Wei-Min , Huang Bo 
Published: March 2022

AbstractPancreatic ductal adenocarcinoma (PDAC) originates from normal pancreatic ducts where digestive juice is regularly produced. It remains unclear how PDAC can escape autodigestion by digestive enzymes. Here we show that human PDAC tumour cells use gasdermin E (GSDME), a pore-forming protein, to mediate digestive resistance. GSDME facilitates the tumour cells to express mucin 1 and mucin 13, which form a barrier to prevent chymotrypsin-mediated destruction. Inoculation of GSDME−/− PDAC cells results in subcutaneous but not orthotopic tumour formation in mice. Inhibition or knockout of mucin 1 or mucin 13 abrogates orthotopic PDAC growth in NOD-SCID mice. Mechanistically, GSDME interacts with and transports YBX1 into the nucleus where YBX1 directly promotes mucin expression. This GSDME–YBX1–mucin axis is also confirmed in patients with PDAC. These findings uncover a unique survival mechanism of PDAC cells in pancreatic microenvironments.
Larvicidal and adulticidal effects of some Egyptian oils against Culex pipiens
Authors: Baz Mohamed M. , Selim Abdelfattah , Radwan Ibrahim Taha , Alkhaibari Abeer Mousa , Khater Hanem F. 
Published: March 2022

AbstractMosquitoes and mosquito-borne diseases represent an increasing global challenge. Plant extract and/or oils could serve as alternatives to synthetic insecticides. The larvicidal effects of 32 oils (1000 ppm) were screened against the early 4th larvae of Culex pipiens and the best oils were evaluated against adults and analyzed by gas chromatography-mass spectrometry (GC mass) and HPLC. All oils had larvicidal activity (60.0–100%, 48 h Post-treatment, and their Lethal time 50 (LT50) values ranged from 9.67 (Thymus vulgaris) to 37.64 h (Sesamum indicum). Oils were classified as a highly effective group (95–100% mortalities), including Allium sativum, Anethum graveolens, Camellia sinensis, Foeniculum vulgare, Nigella sativa, Salvia officinalis, T. vulgaris, and Viola odorata. The moderately effective group (81–92% mortalities) included Boswellia serrata, Cuminum cyminum, Curcuma aromatic, Allium sativum, Melaleuca alternifolia, Piper nigrum, and Simmondsia chinensis. The least effective ones were C. sativus and S. indicum. Viola odorata, Anethum graveolens, T. vulgaris, and N. sativa provide 100% adult mortalities PT with 10, 25, 20, and 25%. The mortality percentages of the adults subjected to 10% of oils (H group) were 48.89%, 88.39%, 63.94%, 51.54%, 92.96%, 44.44%, 72.22%, and 100% for A. sativum, An. graveolens, C. sinensis, F. vulgare, N. sativa, S. officinalis, T. vulgaris, and V. odorata, respectively. Camellia sinensis and F. vulgare were the most potent larvicides whereas V. odorata, T. vulgaris, An. graveolens and N. sativa were the best adulticides and they could be used for integrated mosquito control.
Shared antibiotic resistance and virulence genes in Staphylococcus aureus from diverse animal hosts
Authors: Bruce Spencer A. , Smith Joshua T. , Mydosh Jennifer L. , Ball John , Needle David B. , Gibson Robert , Andam Cheryl P. 
Published: March 2022

AbstractThe emergence of methicillin-resistant Staphylococcus aureus (MRSA) poses an important threat in human and animal health. In this study, we ask whether resistance and virulence genes in S. aureus are homogeneously distributed or constrained by different animal hosts. We carried out whole genome sequencing of 114 S. aureus isolates from ten species of animals sampled from four New England states (USA) in 2017–2019. The majority of the isolates came from cats, cows and dogs. The maximum likelihood phylogenetic tree based on the alignment of 89,143 single nucleotide polymorphisms of 1173 core genes reveal 31 sequence types (STs). The most common STs were ST5, ST8, ST30, ST133 and ST2187. Every genome carried at least eight acquired resistance genes. Genes related to resistance found in all genomes included norA (fluoroquinolone), arlRS (fluoroquinolone), lmrS (multidrug), tet(38) (tetracycline) and mepAR (multidrug and tigecycline resistance). The most common superantigen genes were tsst-1, sea and sec. Acquired antibiotic resistance (n = 10) and superantigen (n = 9) genes of S. aureus were widely shared between S. aureus lineages and between strains from different animal hosts. These analyses provide insights for considering bacterial gene sharing when developing strategies to combat the emergence of high-risk clones in animals.
Genetic diversity and population structure of an African yam bean (Sphenostylis stenocarpa) collection from IITA GenBank
Authors: Shitta Ndenum Suzzy , Unachukwu Nnanna , Edemodu Alex Chukwudi , Abebe Abush Tesfaye , Oselebe Happiness O. , Abtew Wosene Gebreselassie 
Published: March 2022

AbstractAfrican yam bean, AYB (Sphenostylisstenocarpa), is an underutilized legume of tropical Africa. AYB can boost food and nutritional security in sub-Saharan Africa through its nutrient-rich seeds and tubers. However, inadequate information on germplasm with desirable agro-morphological traits, including insufficient data at the genomic level, has prevented the full exploitation of its food and breeding potentials. Notably, assessing the genetic diversity and population structure in a species is a prerequisite for improvement and eventual successful exploitation. The present study evaluated the population structure and genetic diversity of 169 accessions from the International Institute of Tropical Agriculture (IITA) collection using 26 phenotypic characters and 1789 single nucleotide polymorphism (SNP) markers. The phenotypic traits and SNP markers revealed their usefulness in uniquely distinguishing each AYB accession. The hierarchical cluster of phenotypes grouped accessions into three sub-populations; SNPs analysis also clustered the accessions into three sub-populations. The genetic differentiation (FST) among the three sub-populations was sufficiently high (0.14–0.39) and significant at P = 0.001. The combined analysis revealed three sub-populations; accessions in sub-population 1 were high yielding, members in sub-population 2 showed high polymorphic loci and heterozygosity. This study provides essential information for the breeding and genetic improvement of AYB.
Forage grass growth under future climate change scenarios affects fermentation and ruminant efficiency
Authors: Hart Elizabeth H. , Christofides Sarah R. , Davies Teri E. , Rees Stevens Pauline , Creevey Christopher J. , Müller Carsten T. , Rogers Hilary J. , Kingston-Smith Alison H. 
Published: March 2022

AbstractWith an increasing human population access to ruminant products is an important factor in global food supply. While ruminants contribute to climate change, climate change could also affect ruminant production. Here we investigated how the plant response to climate change affects forage quality and subsequent rumen fermentation. Models of near future climate change (2050) predict increases in temperature, CO2, precipitation and altered weather systems which will produce stress responses in field crops. We hypothesised that pre-exposure to altered climate conditions causes compositional changes and also primes plant cells such that their post-ingestion metabolic response to the rumen is altered. This “stress memory” effect was investigated by screening ten forage grass varieties in five differing climate scenarios, including current climate (2020), future climate (2050), or future climate plus flooding, drought or heat shock. While varietal differences in fermentation were detected in terms of gas production, there was little effect of elevated temperature or CO2compared with controls (2020). All varieties consistently showed decreased digestibility linked to decreased methane production as a result of drought or an acute flood treatment. These results indicate that efforts to breed future forage varieties should target tolerance of acute stress rather than long term climate.
A study on diameter-dependent support selection of the tendrils of Cayratia japonica
Authors: Saito Kazuya 
Published: March 2022

AbstractOrganisms make decisions when they perceive cues of varying intensities. In case of climbing plants, the diameter of supports in contact (tree or stem) is an important cue for their growth as plants that coil around a support with large diameter are unable to maintain tensional forces required for continued attachment to the support. The negative association between the diameter and the climbing success has been reported since Darwin published his study on climbing plants. However, it is not known if a climbing plant makes a decision to avoid a support with larger diameter. Here, we tested this possibility by observing the coiling response of tendrils of Cayratia japonica to supports with different diameters. The coiling success of the tendrils was affected by the diameter of the support and the tendril lengths. We propose a decision tree to describe the different phases of the coiling response and demonstrated that the tendrils change their coiling shape depending on the support diameter and the tendril length. To understand the behavioural rules regulating the phase pattern, we constructed a simple model with two assumptions on the tendril movement, (1) when the tendrils receive a contact stimulus, they begin to coil from around the contact point and (2) there is a minimum coiling angle at which the tendrils coil up, once the tendril starts coiling. Image analysis and 3D motion tracking technique revealed that the movement of the tendrils were consistent with the two assumptions of the model. The results suggested that the tendrils flexibly changed the coiling shapes depending on the support diameter and simple behavioural rules could regulate this diameter-dependent response.
Short- and long-term recurrence of early-stage invasive ductal carcinoma in middle-aged and old women with different treatments
Authors: Kao Yuan , Wu Ying-Jhen , Hsu Chien-Chin , Lin Hung-Jung , Wang Jhi-Joung , Tian Yu-Feng , Weng Shih-Feng , Huang Chien-Cheng 
Published: March 2022

AbstractMost new cases and the highest mortality rates of breast cancer occur among middle-aged and old women. The recurrence rate of early-stage invasive ductal carcinoma (IDC) among women aged ≥ 50 years and receiving different treatments remains unclear. Therefore, this study was conducted to determine these rates. We used Surveillance, Epidemiology, and End Results (SEER) data for this nationwide population-based cohort study. All women aged ≥ 50 years and diagnosed with early-stage IDC between 2000 and 2015 were identified and divided into three treatment groups, namely, breast conservation therapy (BCT), mastectomy alone (MAS), and mastectomy with radiation therapy (MAS + RT). The recurrence rates of IDC among these groups were then compared. The BCT group had a lower short-term recurrence risk than the MAS and MAS + RT groups (hazard ratio [HR]: 1.00 vs. 2.90 [95% CI 1.36–2.66] vs. 2.07 [95% CI 0.97–4.44]); however, the BCT group also had a higher long-term recurrence risk than MAS and MAS + RT groups (HR 1.00 vs. 0.30 [95% CI 0.26–0.35] vs. 0.43 [95% CI 0.30–0.63]). The high long-term recurrence rate of the BCT group was especially prominent at the 10- and 15-year follow-ups. The results provide valuable evidence of the most reliable treatment strategy for this population. Further studies including more variables and validation in other countries are warranted to confirm our findings.
Predictors of Covid-19 level of concern among older adults from the health and retirement study
Authors: Beydoun Hind A. , Beydoun May A. , Weiss Jordan , Gautam Rana S. , Hossain Sharmin , Alemu Brook T. , Zonderman Alan B. 
Published: March 2022

AbstractThe purpose of this longitudinal study is to construct a prediction model for Covid-19 level of concern using established Covid-19 socio-demographic, lifestyle and health risk characteristics and to examine specific contributions of obesity-related cardiometabolic health characteristics as predictors of Covid-19 level of concern among a representative sample of U.S. older adults. We performed secondary analyses of existing data on 2872 2006–2020 Health and Retirement Study participants and examined 19 characteristics in relation to the outcome of interest using logistic regression and machine learning algorithms. In mixed-effects ordinal logistic regression models, a history of diabetes, stroke as well as 1–2 cardiometabolic risk factors and/or chronic conditions were associated with greater Covid-19 level of concern, after controlling for confounders. Female sex, birth cohort, minority race, Hispanic ethnicity and total wealth as well as depressive symptoms were associated with higher level of Covid-19 concern, and education was associated with lower level of Covid-19 concern in fully adjusted mixed-effects ordinal logistic regression models. The selected socio-demographic, lifestyle and health characteristics accounted for < 70% of the variability in Covid-19 level of concern based on machine learning algorithms. Independent risk factors for Covid-19 level of concern among U.S. older adults include socio-demographic characteristics and depressive symptoms. Advanced research is needed to identify relevant predictors and elucidate underlying mechanisms of observed relationships.
Simultaneous assessment of spontaneous cage activity and voluntary wheel running in group-housed mice
Authors: Reuser Annika , Wenzel Kristin , Felix Stephan B. , Dörr Marcus , Bahls Martin , Könemann Stephanie 
Published: March 2022

AbstractSmall animal models are frequently used to improve our understanding of the molecular and biological signaling pathways underlying the beneficial effects of physical activity and exercise. Unfortunately, when running wheels are employed, mice and rats are often kept single-housed to determine the individual running distance of each animal. However, social isolation can be stressful for rodents, and may alter an individual’s propensity for or response to exercise. For example, increased stress from single housing may significantly affect the results when investigating systemic metabolic responses to exercise. We have combined two already available and well-established systems, a radiotelemetry system and a running wheel, to determine spontaneous cage activity (SCA) as well as voluntary exercise (VE) levels of the individual animal in group-housed rodents. Further, we developed a simple software tool which allows monitoring and analyzing the data. Specifically, the radiotelemetry-system utilizes radio-frequency identification via a small, implanted chip to determine the location of each animal. Since, in addition to the animals’ position, also the location of the running wheel in the cage is known, the conclusion of which animal is exercising can be drawn. The developed software enables a fast and reliable assignment of the VE data to the individual animal and a simple analysis of the data collected. Hence, our combined method may be used to investigate the beneficial effects of physical activity, as well as the impact of therapeutic interventions on animal behavior in group-housed rodents.
Blood–brain barrier and gut barrier dysfunction in chronic kidney disease with a focus on circulating biomarkers and tight junction proteins
Authors: Hernandez Leah , Ward Liam J. , Arefin Samsul , Ebert Thomas , Laucyte-Cibulskiene Agne ,   GOING-FWD Collaborators, Pilote Louise , Norris Colleen M. , Raparelli Valeria , Kautzky-Willer Alexandra , Herrero Maria Trinidad , Kublickiene Karolina , Heimbürger Olof , Barany Peter , Wennberg Lars , Stenvinkel Peter , Kublickiene Karolina 
Published: March 2022

AbstractKidney failure and associated uraemia have implications for the cardiovascular system, brain, and blood–brain barrier (BBB). We aim to examine BBB disruption, by assessing brain-derived neurotropic factor (BDNF), neuron-specific enolase (NSE) levels, and gut-blood barrier (GBB) disruption by trimethylamine N-oxide (TMAO), in chronic kidney disease (CKD) patients. Additionally, endothelial tight-junction protein expressions and modulation via TMAO were assessed. Serum from chronic kidney disease (CKD) female and male haemodialysis (HD) patients, and controls, were used to measure BDNF and NSE by enzyme-linked immunosorbent assays, and TMAO by mass spectrometry. Immunofluorescent staining of subcutaneous fat biopsies from kidney transplant recipients, and controls, were used to measure microvascular expression of tight-junction proteins (claudin-5, occludin, JAM-1), and control microvasculature for TMAO effects. HD patients versus controls, had significantly lower and higher serum levels of BDNF and NSE, respectively. In CKD biopsies versus controls, reduced expression of claudin-5, occludin, and JAM-1 were observed. Incubation with TMAO significantly decreased expression of all tight-junction proteins in the microvasculature. Uraemia affects BBB and GBB resulting in altered levels of circulating NSE, BDNF and TMAO, respectively, and it also reduces expression of tight-junction proteins that confer BBB maintenance. TMAO serves as a potential candidate to alter BBB integrity in CKD.
Deep cascaded multitask framework for detection of temporal orientation, sentiment and emotion from suicide notes
Authors: Ghosh Soumitra , Ekbal Asif , Bhattacharyya Pushpak 
Published: March 2022

AbstractWith the upsurge in suicide rates worldwide, timely identification of the at-risk individuals using computational methods has been a severe challenge. Anyone presenting with suicidal thoughts, mainly recurring and containing a deep desire to die, requires urgent and ongoing psychiatric treatment. This work focuses on investigating the role of temporal orientation and sentiment classification (auxiliary tasks) in jointly analyzing the victims’ emotional state (primary task). Our model leverages the effectiveness of multitask learning by sharing features among the tasks through a novel multi-layer cascaded shared-private attentive network. We conducted our experiments on a diversified version of the prevailing standard emotion annotated corpus of suicide notes in English, CEASE-v2.0. Experiments show that our proposed multitask framework outperforms the existing state-of-the-art system by 3.78% in the Emotion task, with a cross-validation Mean Recall (MR) of 60.90%. From our empirical and qualitative analysis of results, we observe that learning the tasks of temporality and sentiment together has a clear correlation with emotion recognition.
Disease correlates of rim lesions on quantitative susceptibility mapping in multiple sclerosis
Authors: Marcille Melanie , Hurtado Rúa Sandra , Tyshkov Charles , Jaywant Abhishek , Comunale Joseph , Kaunzner Ulrike W. , Nealon Nancy , Perumal Jai S. , Zexter Lily , Zinger Nicole , Bruvik Olivia , Wang Yi , Sweeney Elizabeth , Kuceyeski Amy , Nguyen Thanh D. , Gauthier Susan A. 
Published: March 2022

AbstractQuantitative susceptibility mapping (QSM), an imaging technique sensitive to brain iron, has been used to detect paramagnetic rims of iron-laden active microglia and macrophages in a subset of multiple sclerosis (MS) lesions, known as rim+ lesions, that are consistent with chronic active lesions. Because of the potential impact of rim+ lesions on disease progression and tissue damage, investigating their influence on disability and neurodegeneration is critical to establish the impact of these lesions on the disease course. This study aimed to explore the relationship between chronic active rim+ lesions, identified as having a hyperintense rim on QSM, and both clinical disability and imaging measures of neurodegeneration in patients with MS. The patient cohort was composed of 159 relapsing–remitting multiple sclerosis patients. The Expanded Disability Status Scale (EDSS) and Brief International Cognitive Assessment for Multiple Sclerosis, which includes both the Symbol Digit Modalities Test and California Verbal Learning Test-II, were used to assess clinical disability. Cortical thickness and thalamic volume were evaluated as imaging measures of neurodegeneration. A total of 4469 MS lesions were identified, of which 171 QSM rim+ (3.8%) lesions were identified among 57 patients (35.8%). In a multivariate regression model, as the overall total lesion burden increased, patients with at least one rim+ lesion on QSM performed worse on both physical disability and cognitive assessments, specifically the Symbol Digit Modalities Test (p = 0.010), California Verbal Learning Test-II (p = 0.030), and EDSS (p = 0.001). In a separate univariate regression model, controlling for age (p < 0.001) and having at least one rim+ lesion was related to more cortical thinning (p = 0.03) in younger patients (< 45 years). Lower thalamic volume was associated with older patients (p = 0.038) and larger total lesion burden (p < 0.001); however, the association did not remain significant with rim+ lesions (p = 0.10). Our findings demonstrate a novel observation that chronic active lesions, as identified on QSM, modify the impact of lesion burden on clinical disability in MS patients. These results support further exploration of rim+ lesions for therapeutic targeting in MS to reduce disability and subsequent neurodegeneration.
Dome-arrayed chitosan/PVA hydrogel-based solar evaporator for steam generation
Authors: Zhu Minyue , Liu Xiaojie , Tian Yanpei , Caratenuto Andrew , Chen Fangqi , Zheng Yi 
Published: March 2022

AbstractWater evaporation systems with solar energy as the primary driving energy have received extensive attention in recent years. This work studies the preparation method and performance of hydrogel evaporators using chitosan and polyvinyl alcohol (PVA) as a framework and carbon nanoparticles (CNPs) as the photothermal material. The evaporation rate of CPC (chitosan/PVA and CNPs) hydrogel obtained reaches 2.28 kg m−2 h−1. Simultaneously, a three-dimensional structure is designed based on the two-dimensional double-layer evaporation system in this study. An evaporator with a tiny-pool structure and a hydrogel with a dome-arrayed structure is designed. These two structures achieve highly efficient evaporation rates of 2.28 kg m−2 h−1 and 3.80 kg m−2 h−1, respectively. These optimized designs improve the evaporation rate of the overall system by ~ 66.7%. The developed evaporation devices provide a promising pathway for developing the double-layer evaporators, which promote the new development of water purification with a solar-driven evaporation system.