bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal inhibition of electron transport chain attenuates stress-induced cellular senescence by prolonged disturbance of proteostasis in human fibroblasts
Authors: Takenaka Yasuhiro , Inoue Ikuo , Hirasaki Masataka , Ikeda Masaaki , Kakinuma Yoshihiko 
Published: November 2022

AbstractWe previously developed a stress-induced premature senescence (SIPS) model in which normal human fibroblast MRC-5 cells were treated with either the proteasome inhibitor MG132 or the V-ATPase inhibitor bafilomycin A1 (BAFA1). To elucidate the involvement of mitochondrial function in our SIPS model, we treated cells with an inhibitor of electron transport chain (ETC) complexes I, III, or a mitochondrial uncoupler reagent along with MG132 or BAFA1 and evaluated the induction of premature senescence. SIPS induced by MG132 or BAFA1 was partially attenuated by co-treatment with antimycin A (AA) and rotenone, but not carbonyl cyanide 3-chlorophenylhydrazone (CCCP), in which intracellular reactive oxygen species (ROS) levels, acute mitochondrial unfolded protein responses, and accumulation of protein aggregates were remarkably suppressed. Co-treatment with AA also reversed the temporal depletion of SOD2 in the mitochondrial fraction on day 1 of MG132 treatment. Furthermore, co-treatment with AA suppressed the induction of mitophagy in MG132-treated cells and enhanced mitochondrial biogenesis. These findings provide evidence that the temporal inhibition of mitochondrial respiration exerts protective effects against the progression of premature senescence caused by impaired proteostasis.Graphical abstractGRAPHICAL ABSTRACTCellular senescence is induced by prolonged inhibition of proteasome or lysosome function using MG132 and bafilomycin A1 (BAFA1), respectively. When cells were co-treated with a mitochondrial respiration inhibitor, antimycin A, and MG132 or BAFA1, oxidative stress, mitochondrial unfolded protein response (UPRmt), accumulation of protein aggregates, and mitophagy were suppressed whereas mitochondrial biogenesis was enhanced, resulting in the attenuation of stress-induced cellular senescence (SIPS).
Simulating the effect of ankle plantarflexion and inversion-eversion exoskeleton torques on center of mass kinematics during walking
Authors: Bianco Nicholas A. , Collins Steven H. , Liu Karen , Delp Scott L. 
Published: November 2022

AbstractWalking balance is central to independent mobility, and falls due to loss of balance are a leading cause of death for people 65 years of age and older. Bipedal gait is typically unstable, but healthy humans use corrective torques to counteract perturbations and stabilize gait. Exoskeleton assistance could benefit people with neuromuscular deficits by providing stabilizing torques at lower-limb joints to replace lost muscle strength and sensorimotor control. However, it is unclear how applied exoskeleton torques translate to changes in walking kinematics. This study used musculoskeletal simulation to investigate how exoskeleton torques applied to the ankle and subtalar joints alter center of mass kinematics during walking. We first created muscle-driven walking simulations using OpenSim Moco by tracking experimental kinematics and ground reaction forces recorded from five healthy adults. We then used forward integration to simulate the effect of exoskeleton torques applied to the ankle and subtalar joints while keeping muscle excitations fixed based on our previous tracking simulation results. Exoskeleton torque lasted for 15% of the gait cycle and was applied between foot-flat and toe-off during the stance phase, and changes in center of mass kinematics were recorded when the torque application ended. We found that changes in center of mass kinematics were dependent on both the type and timing of exoskeleton torques. Plantarflexion torques produced upward and backward changes in velocity of the center of mass in mid-stance and upward and forward velocity changes near toe-off. Eversion and inversion torques primarily produced lateral and medial changes in velocity in mid-stance, respectively. Intrinsic muscle properties reduced kinematic changes from exoskeleton torques. Our results provide mappings between ankle plantarflexion and inversion-eversion torques and changes in center of mass kinematics which can inform designers building exoskeletons aimed at stabilizing balance during walking. Our simulations and software are freely available and allow researchers to explore the effects of applied torques on balance and gait.
Subcellular protein localisation ofTrypanosoma bruceibloodstream form-upregulated proteins maps stage-specific adaptations
Authors: Halliday Clare , Dean Samuel , Sunter Jack Daniel , Wheeler Richard John 
Published: November 2022

AbstractGenome-wide subcellular protein localisation inTrypanosoma brucei, through our TrypTag project, has comprehensively dissected the molecular organisation of this important pathogen. Powerful as this resource is,T. bruceihas multiple developmental forms and we previously only analysed the procyclic form. This is an insect life cycle stage, leaving the mammalian bloodstream form unanalysed. The expectation is that between life stages protein localisation would not change dramatically (completely unchanged or shifting to analogous stage-specific structures). However, this has not been specifically tested. Similarly, which organelles tend to contain proteins with stage-specific expression can be predicted from known stage specific adaptations but has not been comprehensively tested. Here, we used endogenous tagging to determine the sub-cellular localisation of the majority of proteins encoded by transcripts significantly upregulated in the bloodstream form and compared them to the existing localisation data in procyclic forms. This confirmed the localisation of known and identified the localisation of novel stage-specific proteins, giving a map of which organelles tend to contain stage specific proteins: the mitochondrion for the procyclic form, and the endoplasmic reticulum, endocytic system and cell surface in the bloodstream form.
The Siglec-sialic acid-axis is a target for innate immunotherapy of glioblastoma
Authors: Schmassmann Philip , Roux Julien , Buck Alicia , Tatari Nazanin , Hogan Sabrina , Wang Jinyu , Lee Sohyon , Snijder Berend , Martins Tomás A. , Ritz Marie-Françoise , Shekarian Tala , Kaymak Deniz , McDaid Marta , Weller Michael , Weiss Tobias , Läubli Heinz , Hutter Gregor 
Published: November 2022

AbstractGlioblastoma (GBM) is the most aggressive form of primary brain tumor, for which effective therapies are urgently needed. Cancer cells are capable of evading clearance by phagocytes such as microglia and monocyte-derived cells through engaging tolerogenic programs. Here, we found that high level of Siglec-9 expression correlates with reduced survival in GBM patients. Using conditional knockouts of Siglec-E, the murine functional homologue of Siglec-9, together with single-cell RNA sequencing, we demonstrated significant pro-phagocytosis effects in microglia and monocyte-derived cells in the absence of Siglec-E. Loss of Siglec-E on monocyte-derived cells enhances antigen cross-presentation and production of pro-inflammatory cytokines, resulting in more efficient T cell priming. This bridging of innate and adaptive responses delays tumor growth and results in prolonged survival. Further, we showed synergistic activity of Siglec-E blockade in combinatorial immunotherapies and demonstrate its translational potential against GBM.
Signatures of kin selection in a natural population of the bacteriaBacillus subtilis
Authors: Belcher Laurence J. , Dewar Anna E. , Hao Chunhui , Ghoul Melanie , West Stuart A. 
Published: November 2022

AbstractLaboratory experiments have suggested that bacteria perform a range of cooperative behaviours, which are favoured because they are directed towards relatives (kin selection). However, there is a lack of evidence for cooperation and kin selection in natural bacterial populations. Molecular population genetics offers a promising method to study natural populations, because theory predicts that kin selection will lead to relaxed selection, which will result in increased polymorphism and divergence at cooperative genes. Examining a natural population ofBacillus subtilis, we found consistent evidence that putatively cooperative traits have higher polymorphism and greater divergence than putatively private traits expressed at the same rate. In addition, we were able to eliminate alternative explanations for these patterns, and found more deleterious mutations in genes controlling putatively cooperative traits. Overall, our results suggest cooperation favoured by kin selection, with an average relatedness ofr=0.77 between interacting individuals.
Structural Insights into Branch Site Proofreading by Human Spliceosome
Authors: Zhang Xiaofeng , Zhan Xiechao , Bian Tong , Yang Fenghua , Li Pan , Lu Yichen , Xing Zhihan , Zhang Qiangfeng Cliff , Shi Yigong 
Published: November 2022

AbstractSelection of the pre-mRNA branch site (BS) by U2 snRNP is crucial to prespliceosome (A complex) assembly. The RNA helicase PRP5 proofreads BS selection; but the underlying mechanism remains unclear. Here we report the atomic structures of two sequential complexes leading to prespliceosome assembly: human 17S U2 snRNP and a cross-exon pre-A complex. PRP5 is anchored on 17S U2 snRNP mainly through occupation of the RNA path of SF3B1 by an acidic loop of PRP5; the helicase domain of PRP5 associates with U2 snRNA; the BS-interacting stem loop (BSL) of U2 snRNA is shielded by the splicing factor TAT-SF1, unable to engage the BS. In the pre-A complex, an initial U2/BS duplex is formed; the translocated helicase domain of PRP5 stays with U2 snRNA; the acidic loop still occupies the RNA path. The pre-A conformation is specifically stabilized by the splicing factors SF1, DNAJC8 and SF3A2. Cancer-derived mutations in SF3B1 damage its association with PRP5, compromising BS proofreading. Together, these findings reveal key insights into prespliceosome assembly and BS selection/proofreading by PRP5.
Planning comprehensiveness and resource consumption in Chinese small enterprises
Authors: Rickards Robert C. , Ritsert Rolf 
Published: November 2022

Purpose
This paper explores the comprehensiveness of management accountants’ planning and the resource consumption associated with it in Chinese small enterprises (SEs).

Design/methodology/approach
The methodology involves correlation and regression analyses of online survey responses.

Findings
The study findings are as follows: planning comprehensiveness and the resource consumption associated with it vary greatly across Chinese SEs; there is a trade-off between these two aspects of planning; and provision of linkages within and across plan types, employment of valuation analytics and the use of future-oriented techniques and tools, as well as joint venturing with a foreign partner explain roughly three-quarters of the observed differences in planning behavior.

Research limitations/implications
With no readily accessible source to ensure random selection of the units of analysis, this study relies on a convenience sample. Participants’ survey responses may contain a residual element of key informant bias.

Originality/value
This study uses primary data exclusively. It demonstrates a general trade-off between the comprehensiveness of management accountants’ planning and the associated resource consumption in Chinese SEs. Its novelty also derives from an approach that explains intercompany differences in the observed behavior via variables heretofore seldom used in empirical research.
Dynamic behavior analysis of cascaded buck converter and boost converter system
Authors: Xie Fan , Geng Xinyue , Li Guozheng , Ji Huayv , Luo Zhenxiong 
Published: November 2022

Purpose
Cascaded DC-DC converters system is the main structure of distributed power system, and it has complex nonlinear phenomena during operation, which affect the power quality. Therefore, the dynamic behavior of the cascaded buck converter and boost converter system, as one of the typical cascaded DC-DC converters systems is analyzed.

Design/methodology/approach
Firstly, the studied cascaded system of the buck converter with peak current control and the boost converter with PI current control is introduced and its discrete modeling is built. Then, the Jacobian matrix of the cascaded system is calculated to research the stability when the parameter change. Finally, simulation by PSIM and experiments are carried out to verify the theoretical analysis.

Findings
The coexistence of fast and slow time scale bifurcations with the changes of reference current and input voltage are studied in the cascaded system, and using simulation analysis to further study the sensitivity of the inductor current of the front-stage converter and back-stage converter to different parameters.

Originality/value
A discrete model of the cascaded buck converter and boost converter system is established, and its dynamic behavior is analyzed in detail for the first time.
Empirical validation of IR sustainability model: leveraging on a PLS-SEM approach
Authors: Adam Usman Ahmed , Kaur Kiran 
Published: November 2022

PurposeThis study aims to validate a proposed conceptual model for the implementation of sustainable institutional repositories (IRs) in Nigeria.Design/methodology/approachA quantitative approach shaped the survey research design. This study used structural equation modeling (SEM) analysis to evaluate the proposed model. The population of the study comprises 117 librarians, information technology staff and researchers knowledgeable about IR implementation status across 14 public universities in Nigeria. The data was collected using an online survey. The Smart-PLS v3.3 software was used to facilitate the analysis.FindingsThe findings indicate that the nine identified factors of the IR sustainability model have a significant influence on the implementation of sustainable IRs. This signified that the model has adequately depicted the relationship between the implementation of sustainable IRs and the identified factors.Originality/valueThis study provides an integrated synthesis of factors that influence the implementation of sustainable IRs. This study also presents the first-ever empirically validated model for sustainable IRs. The findings of this study addressed the challenge of implementing sustainable IRs and institutionalized the idea of IRs’ sustainability assessment.
Ethical analysis of architecture on structural irregularities in major earthquakes in Turkey
Authors: Dallı Mustafa , Soyluk Asena 
Published: November 2022

Purpose
The aim of this study is to question the relationship between architectural ethical codes and faults in earthquakes. Earthquakes have devastating effects on all societies in history and today. And the relationship and importance of the architect and building, one of the most important roles of these destructive effects, is once again revealed in every earthquake. Although there are some restrictions or warnings for architects and the architectural profession to reduce this destructive effect in many regulations and ethical codes, it is possible to see the defects caused by architectural design and the destruction caused by these defects in every new earthquake.

Design/methodology/approach
In this study, the most destructive earthquakes in Turkey in the past 20 years (Bingöl, Van, Elazig and Izmir) and the 1999 Marmara earthquake, which was the most destructive earthquake in Turkey’s recent history, and the damages occurred in these earthquakes and their causes were examined. Although the scope of the study is “destructive earthquakes that have occurred in the past 20 years in Turkey”, the Marmara Earthquake, which occurred in 1999, when the destructive effect of the earthquake was seen the most and architectural design errors were intense, was also included in the scope of the study. And to have a more comprehensive understanding of how these defects are examined in terms of ethical codes and to make a more comprehensive comparison, ethical codes from different countries in the world have been researched and a review has been made on topics such as public welfare, human rights and raising the standard of the profession.

Findings
This study concludes by reviewing the key factors learned from the examined ethical codes of different countries. Finding ethical codes of different countries was challenging to gain approval. In addition, the study ends with recommendations in terms of questioning the regulations and education curriculum relations on a country basis.

Originality/value
This study, which targets architect candidates who carry out the profession of architecture and continue their architectural education, evaluates the architectural design flaws seen in earthquakes through ethical codes and forms a basis for further studies.
Design of transformable transitional shelter for post disaster relief
Authors: Cerrahoğlu Merve , Maden Feray 
Published: November 2022

Purpose
The most important need after natural disasters is the sheltering. However, most of the existing temporary shelters do not meet all requirements for long-term use and not provide adequate flexibility within the space. This paper aims to develop a transitional postdisaster shelter transforming from a closed shape to an expanded form in response to changing functional and spatial needs of disaster victims. The study also proposes alternative unit combinations for various functions, and settlement layouts to create a comfortable living environment for occupants.

Design/methodology/approach
The research methodology is based on theoretical and design frameworks which requires inductive and deductive approaches. Forming the background of the study, the theoretical framework consists of four parts which are literature review on temporary shelters presenting state-of-the-art; determination of design guidelines and strategies based on shelter standards; identification of technical requirements; and analysis of existing temporary shelters. Having three parts, the design framework includes design of transformable transitional shelter based on three-dimensional modeling, creation of different unit combinations to be used for various purposes and development of settlement layouts as case studies.

Findings
The analysis conducted in this study demonstrates that most of the existing temporary shelters have limited geometric configurations and major problems in terms of their performance, transportation and storage. On the other hand, the transformable shelter proposed by the authors can provide form and spatial flexibilities thanks to its expansion properties, occupy less space for transportation, easily be transported to any desired location in its compact state and be customized according to user needs. Several units can be combined either to serve larger families or to be used for different functions.

Originality/value
This paper contributes to the literature as presenting not only a theoretical framework on temporary shelters but also a design framework on transformable shelter design for the ones who are willing to develop similar transformable shelters based on the determined guidelines, strategies and requirements.
Dynamic capabilities as a strategic flexibility enabler: organizational responsiveness to COVID-19
Authors: Muneeb Dilnaz , Khattak Amira , Wahba Karim , Abdalla Shahira , Ahmad Syed Zamberi 
Published: November 2022

PurposeTo cope with the existing pandemic situation and to be organizationally responsive, firms need to be strategically flexible, where they need to develop dynamic capabilities (DCs) by continuously reconfiguring their resource base. To address such challenges, firms heavily rely on information and communication technologies (ICT) because of advancement in disruptive technologies. This study aims to explore techniques used by higher education institutional (HEI) leaders to successfully address challenges posed by global disruption, i.e. COVID-19 with the help of advanced ICT software such as Zoom, Google Meet and Microsoft Teams.Design/methodology/approachA qualitative approach was adopted to explore strategic factors such as strategic flexibility (SF) and DC that disclose shortcomings in the current extant literature. A total of 15 interviews were conducted with heads of departments of HEIs in the United Arab Emirates. Data were analyzed using NVivo software.FindingsThe findings suggested three dimensions of SF (resources, operational and collaborative) and four dimensions of DC (strategic planning, innovative, adaptability and technological) for firms to adopt to be strategically flexible, where DC serves as building blocks of SF.Originality/valueThis research provides a framework as an avenue for future researchers and practitioners on how to strategically manage their resources and be strategically flexible in turbulent environment such as pandemics. Theory-based investigations on strategic capabilities and DC from resource-based perspective are still under-researched, emphasizing the need for theoretically based research on strategic responsiveness, especially during the times of environmental complexities such as COVID-19 pandemics. This research enriches strategic management research by exploring the important antecedents of organizational responsiveness, including SF and DC together with the support of human factor, i.e. leadership qualities of HEIs managers. This study, to the best of the authors’ knowledge, is among the first to systematically explore main dimensions of DC and SF based on the resource-based theory of strategic management in the Middle Eastern context.
Family business branding from a signaling theory perspective: an integrative framework
Authors: Galvagno Marco , Pisano Vincenzo , Strano Sonia M. 
Published: November 2022

Purpose
This study aims to review family business branding research, elaborate a new framework integrating family business branding and corporate brand management literature and finally identify future research directions.

Design/methodology/approach
A bibliometric analysis was conducted to present the main research topics within family business branding.

Findings
The contributions of this study are threefold. First, this study maps the main themes of family business branding research and highlights its fragmented nature. Second, this study proposes an overarching framework based on signaling theory, attempts to bridge the family business branding and corporate brand management literature and provides a lead for future research. Third, this study stresses the role of brand construct in family business branding.

Originality/value
This study represents an important step in the identification of a new theoretical framework that best fits the investigation of family business branding.