bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disruption of the primary salicylic acid hydroxylases in rice enhances broad‐spectrum resistance against pathogens
Authors: Zhang Yanjun , Yu Qilu , Gao Shilei , Yu Ningning , Zhao Li , Wang Jinbin , Zhao Jiangzhe , Huang Peng , Yao Linbo , Wang Mo , Zhang Kewei 
Published: April 2022

AbstractSalicylic acid (SA) is a crucial hormone involved in plant immunity. Rice (Oryza sativa) maintains high SA levels that are not induced by pathogens. However, the roles of SA in rice immunity and yield remain largely unknown. Here, we identified SA 5‐hydroxylases 1 (OsS5H1) and 2 (OsS5H2) as the primary enzymes engaged in catalysing SA to 2,5‐dihydroxybenzoic acid (2,5‐DHBA) in rice. SA levels were significantly increased in the oss5h mutants, while they were dramatically decreased in the OsS5H1 and OsS5H2 overexpression lines. The mutants were resistant, whereas the overexpression lines were susceptible to Pyricularia oryzae and Xanthomonas oryzae pv. Oryzae. Moreover, the pathogen‐associated molecular patterns‐triggered immunity responses, including reactive oxygen species burst and callose deposition, were enhanced in all the mutants and compromised in the overexpression lines. Quantification of the agronomic traits of the oss5h mutants grown in the paddy fields demonstrated that the grain number per panicle was decreased as the SA levels increased; however, the tiller number and grain size were enhanced, resulting in no significant yield penalty. Collectively, we reveal that mildly increasing SA content in rice can confer broad‐spectrum resistance without yield penalty and put new insights into the roles of SA in immunity and growth.
The WRKY10‐VQ8 module safely and effectively regulates rice thermotolerance
Authors: Chen Sique , Cao Hongrui , Huang Baolin , Zheng Xiujuan , Liang Kangjing , Wang Guo‐Liang , Sun Xinli 
Published: April 2022

AbstractWRKY transcription factors (TFs) play crucial roles in biotic and abiotic stress responses. However, their roles in thermal response are still largely elusive, especially in rice. In this study, we revealed the functions of WRKY10 TF and VQ8 protein containing VQ motif in rice thermotolerance. Overexpression of WRKY10 or loss of VQ8 function increases thermosensitivity, whereas conversely, overexpression of VQ8 or loss of WRKY10 function enhances thermotolerance. Overexpression of WRKY10 accelerates reactive oxygen species (ROS) accumulation in chloroplasts and apoplasts, and it also induces the expression of heat shock TF and protein genes. We also found that WRKY10 regulates nuclear DNA fragmentation and hypersensitive response by modulating NAC4 TF expression. The balance between destructive and protective responses in WRKY10‐overexpression plant is more fragile and more easily broken by heat stress compared with wild type. In vitro and in vivo assays revealed that VQ8 interacts with WRKY10 and inhibits the transcription activity via repressing its DNA‐binding activity. Our study demonstrates that WRKY10 negatively regulates thermotolerance by modulating the ROS balance and the hypersensitive response and that VQ8 functions antagonistically to positively regulate thermotolerance. The functional module of WRKY10‐VQ8 provides safe and effective regulatory mechanisms in the heat stress response.
Leaf water potential measurements using the pressure chamber: Synthetic testing of assumptions towards best practices for precision and accuracy
Authors: Rodriguez‐Dominguez Celia M. , Forner Alicia , Martorell Sebastia , Choat Brendan , Lopez Rosana , Peters Jennifer M. R. , Pfautsch Sebastian , Mayr Stefan , Carins‐Murphy Madeline R. , McAdam Scott A. M. , Richardson Freya , Diaz‐Espejo Antonio , Hernandez‐Santana Virginia , Menezes‐Silva Paulo E. , Torres‐Ruiz Jose M. , Batz Timothy A. , Sack Lawren 
Published: April 2022

AbstractLeaf water potential (ψleaf), typically measured using the pressure chamber, is the most important metric of plant water status, providing high theoretical value and information content for multiple applications in quantifying critical physiological processes including drought responses. Pressure chamber measurements of ψleaf (ψleafPC) are most typical, yet, the practical complexity of the technique and of the underlying theory has led to ambiguous understanding of the conditions to optimize measurements. Consequently, specific techniques and precautions diversified across the global research community, raising questions of reliability and repeatability. Here, we surveyed specific methods of ψleafPC from multiple laboratories, and synthesized experiments testing common assumptions and practices in ψleafPC for diverse species: (i) the need for equilibration of previously transpiring leaves; (ii) leaf storage before measurement; (iii) the equilibration of ψleaf for leaves on bagged branches of a range of dehydration; (iv) the equilibration of ψleaf across the lamina for bagged leaves, and the accuracy of measuring leaves with artificially ‘elongated petioles’; (v) the need in ψleaf measurements for bagging leaves and high humidity within the chamber; (vi) the need to avoid liquid water on leaf surfaces; (vii) the use of ‘pulse’ pressurization versus gradual pressurization; and (viii) variation among experimenters in ψleafPC determination. Based on our findings we provide a best practice protocol to maximise accuracy, and provide recommendations for ongoing species‐specific tests of important assumptions in future studies.
Accuracy of noninvasive fibrosis scores in predicting advanced fibrosis in pediatric patients after liver transplantation
Authors: Habash Nawras , Ezaizi Yamen , Kak Ibrahim , Conjeevaram Selvakumar Praveen Kumar , Lopez Rocio , Alkhouri Naim , Kabbany Mohammad Nasser 
Published: April 2022

AbstractBackgroundLiver biopsy is the gold standard to stage fibrosis in liver disease. Several scoring systems have been studied to predict advanced fibrosis in liver disease. Those scores have not been validated in pediatric liver transplant patients.AimEvaluate the performance of three fibrosis scores (FSc) in assessing the presence of advanced fibrosis (AF) in children after orthotopic liver transplantation (OLT).MethodsPatients < 20 years of age who underwent liver biopsy post‐OLT with laboratory values within 1 month of the biopsy were included. Fibrosis was determined by an experienced pathologist (F0‐4). We defined AF as F3‐4. The following FSc were calculated: AST/ALT ratio, APRI, and FIB‐4 index. Receiver operating characteristic curve analysis was done to assess the FSc performance in predicting AF.ResultsA total of 232 biopsies were analyzed, of those 42 (18.1%) showed AF (F3‐4). FIB‐4 was significantly higher in patients with AF compared to those without AF [median value of 1.1 [0.7, 3.0] and 0.6 [0.2, 1.4], respectively (p = .02)]; however, FIB‐4 had satisfactory accuracy to diagnose AF with significant overlap and AUC of 0.68 (CI 0.56–0.81). Cutoff points of 0.2 and 3.03 were used to rule in and rule out AF, respectively. AST/ALT and APRI were not significantly different between patients with and without AF.ConclusionEven though FIB‐4 had satisfactory accuracy in detecting AF in pediatric transplant patients, noninvasive hepatic FSc developed in adults still performed poorly. Our results highlight the need to develop a reliable pediatric FSc.
To switch or to repeat? Commonalities and differences in the electrophysiological correlates of preparation for voluntary and forced task choices
Authors: Jurczyk Vanessa , Steinhauser Robert , Dreisbach Gesine , Steinhauser Marco 
Published: April 2022

AbstractWhen switching tasks in the laboratory, either the experimenter or the participant can decide which task comes next. So far, this kind of forced and voluntary task switching is usually investigated in isolation. However, in our everyday life, switching between different tasks and goals often depends both on current situational demands and on our intentions. While research has mainly focused on differences between forced and voluntary switching, it is still unclear whether, and if so, which neural processes are shared between both switch types. To identify these, we compared electrophysiological preparatory activity in blocks of randomly intermixed voluntary and forced task‐switching trials. We further manipulated the forced switch rate (20% vs. 80%) between blocks to de‐confound voluntariness with switch frequency and to investigate how switch frequency effects influence preparatory potentials. ERP analysis revealed an enhanced early parietal activity pattern in the P3b time window on voluntary trials, possibly reflecting early traces of a decision process. A later pre‐target negativity was enhanced on forced as compared to voluntary trials. Multivariate pattern analyses revealed that a common preparatory activity on both forced and voluntary switch trials can be found in the switch positivity time window, which we interpreted as an index of a common endogenous task preparation process.
Experimental Implementation of Energy Closure Analysis for Reduced Order Modeling
Authors: Bhattacharyya Suparno , Cusumano Joseph. P. 
Published: April 2022

Abstract
Reduced order models (ROMs) provide an efficient, kinematically condensed representation of computationally expensive high-dimensional dynamical systems; however, their accuracy depends crucially on the accurate estimation of their dimension. We here demonstrate how the energy closure criterion, developed in our prior work, can be experimentally implemented to accurately estimate the dimension of ROMs obtained using the proper orthogonal decomposition (POD). We examine the effect of using discrete data with and without measurement noise, as will typically be gathered in an experiment or numerical simulation, on estimating the degree of energy closure on a candidate reduced subspace. To this end, we used a periodically kicked Euler–Bernoulli beam with Rayleigh damping as the model system and studied ROMs obtained by applying POD to discrete displacement field data obtained from simulated numerical experiments. An improved method for quantifying the degree of energy closure is presented: the convergence of energy input to or dissipated from the system is obtained as a function of the subspace dimension, and the dimension capturing a predefined percentage of either energy is selected as the ROM dimension. This method was found to be more robust to data discretization error and measurement noise. The data-processing necessary for the experimental application of energy closure analysis is discussed in detail. We show how ROMs formulated from the simulated data using our approach accurately capture the dynamics of the beam for different sets of parameter values.
Dynamic Modeling and Transient Response of a Rigid-Body Inductrack Maglev System
Authors: Wang Ruiyang , Yang Bingen 
Published: April 2022

Abstract
The Inductrack system provides a novel way to achieve magnetic levitation by using Halbach arrays of permanent magnets (PMs). Due to the complexities of the nonlinear electro-magneto-mechanical coupling in the system, most previous analyses of the Inductrack system rely on steady-state results and consequently cannot fully capture the dynamic behaviors of the system in transient scenarios. In this article, a new three degrees-of-freedom (3DOF) transient model of the Inductrack system is proposed. This model describes the rigid-body motion of the Inductrack vehicle with axial (longitudinal) and vertical (transverse) displacements and pitch rotation, and it is derived without any assumption of steady-state quantities. Compared to a recently available 2DOF lumped-mass model developed by the authors, the inclusion of the pitch rotation in the new model results in a much more complicated mechanism of electro-magneto-mechanical coupling. Numerical results show that the pitch rotation can have a significant effect on the dynamic response and stability of the Inductrack system, which necessities vibration control for the safe operation of the Inductrack system.
Domestic and Foreign Islamic Studies in the 1980s and the Soviet–Afghan War
Authors: Guseva Yu. N. 
Published: April 2022

Abstract
The author of this article holds that the entry of Soviet troops into Afghanistan was used by the opponents of the Soviet Union both in the West and in Central Asia to revive pan-Turkic ideas and to present the Soviet Union as an opponent of Islam in all its manifestations. To a certain extent, this situation was based on a serious study of the history and current state of Islam abroad. Soviet Islamic studies turned out to be on the far periphery of scientific interests. In the context of the outbreak of the conflict, domestic Islamic studies were found to be catching up. Research did not support knowledge at the level necessary for making political decisions. Politicians did not listen to experts; on the contrary, scientists tried to guess what the government wanted to hear from them.
On the Circumstances of the Decision to Deploy Troops to Afghanistan
Authors: Pikhoya R. G. 
Published: April 2022

Abstract
This article examines the context in which the highest political leadership of the Soviet Union made the decision to deploy troops to Afghanistan and points out the utmost level of secrecy of the corresponding resolution adopted by the Political Bureau of the Central Committee of the Communist Party of the Soviet Union (CC CPSU Politburo). The inconsistency of the drafting of this decision, “Concerning the Situation in ‘A,’” with the Politburo document drafting norms then in force, is noted.
Sjogren syndrome presenting as atrioventricular block in an adult
Authors: Ehtesham Moiz , Fortune Kathryn , Shabbir Muhammad Asim , Peredo-Wende Ruben 
Published: April 2022

A woman in her late teens with a history of Juvenile idiopathic arthritis (JIA) and ongoing sicca symptoms presented with syncope. Upon admission, she was found to be bradycardic with a second-degree atrioventricular (AV) block. After infectious, structural and metabolic aetiologies had been ruled out, she was worked up for rheumatologic causes.Our patient had elevated titres of anti-Sjogren syndrome (SS) antibodies anti-Ro antibodies and was diagnosed with AV block secondary to SS. She was treated with a permanent pacemaker. Patient was followed up in clinic where she denied further syncopal episodes and was started on secretagogues for sicca symptoms.
Evaluating algorithmic fairness in the presence of clinical guidelines: the case of atherosclerotic cardiovascular disease risk estimation
Authors: Foryciarz Agata , Pfohl Stephen R , Patel Birju , Shah Nigam 
Published: April 2022

ObjectivesThe American College of Cardiology and the American Heart Association guidelines on primary prevention of atherosclerotic cardiovascular disease (ASCVD) recommend using 10-year ASCVD risk estimation models to initiate statin treatment. For guideline-concordant decision-making, risk estimates need to be calibrated. However, existing models are often miscalibrated for race, ethnicity and sex based subgroups. This study evaluates two algorithmic fairness approaches to adjust the risk estimators (group recalibration and equalised odds) for their compatibility with the assumptions underpinning the guidelines’ decision rules.MethodsUsing an updated pooled cohorts data set, we derive unconstrained, group-recalibrated and equalised odds-constrained versions of the 10-year ASCVD risk estimators, and compare their calibration at guideline-concordant decision thresholds.ResultsWe find that, compared with the unconstrained model, group-recalibration improves calibration at one of the relevant thresholds for each group, but exacerbates differences in false positive and false negative rates between groups. An equalised odds constraint, meant to equalise error rates across groups, does so by miscalibrating the model overall and at relevant decision thresholds.DiscussionHence, because of induced miscalibration, decisions guided by risk estimators learned with an equalised odds fairness constraint are not concordant with existing guidelines. Conversely, recalibrating the model separately for each group can increase guideline compatibility, while increasing intergroup differences in error rates. As such, comparisons of error rates across groups can be misleading when guidelines recommend treating at fixed decision thresholds.ConclusionThe illustrated tradeoffs between satisfying a fairness criterion and retaining guideline compatibility underscore the need to evaluate models in the context of downstream interventions.
Long-term cardiovascular risk prediction in the emergency department: a mixed-methods study protocol
Authors: Reynard Charles , McMillan Brian , Jafar Anisa , Heagerty Anthony , Martin Glen Philip , Kontopantelis Evangelos , Body Richard 
Published: April 2022

IntroductionCardiovascular disease (CVD) remains one of the leading causes of preventable death in Europe, therefore any opportunity to intervene and improve care should be maximised. Known CVD risk factors are routinely collected in the emergency department (ED), yet they are often not acted on. If the risk factors have prognostic value and a pathway can be created, then this would provide more holistic care for patients and reduce health system inefficiency.Methods and analysisIn this mixed-methods study, we will use quantitative methods to investigate the prognostic characteristics of routinely collected data for long-term CVD outcomes, and qualitative methods to investigate how to use and implement this knowledge. The quantitative arm will use a database of approximately 21 000 chest pain patient episodes with a mean follow-up of 7.3 years. We will use Cox regression to evaluate the prognostic characteristics of routinely collected ED data for long-term CVD outcomes. We will also use a series of semi-structured interviews to co-design a prototype care pathway with stakeholders via thematic analysis. To enable the development of prototypes, themes will be structured into a logic model consisting of situation, inputs, outputs and mechanism.Ethics and disseminationThis work has been approved by Research Ethics Committee (Wales REC7) and the Human Research Authority under reference 19/WA/0312 and 19/WA/0311. It has also been approved by the Confidentiality Advisory Group reference 19/CAG/0209. Dissent recorded in the NHS’ opt-out scheme will be applied to the dataset by NHS Digital. This work will be disseminated through peer-review publication, conference presentation and a public dissemination strategy.Trial registration numberISRCTN41008456.Protocol versionV.1.0—7 June 2021.
Hypertension, its correlates and differences in access to healthcare services by gender among rural Zambian residents: a cross-sectional study
Authors: Tateyama Yukiko , Techasrivichien Teeranee , Musumari Patou Masika , Suguimoto S. Pilar , Ongosi Anita Nyaboke , Zulu Richard , Dube Christopher , Ono-Kihara Masako , Kihara Masahiro 
Published: April 2022

ObjectivesTo examine the prevalence of hypertension and access to related healthcare services among rural residents of Mumbwa district in Zambia.DesignCross-sectional study with probability cluster sampling.SettingRural Zambia.ParticipantsWe recruited 690 residents from Mumbwa district aged 25–64 years who had been living in the study area for ≥6 months and had adopted the lifestyle of the study area. Pregnant women and women who had given birth in the past 6 months were excluded. The data collection—questionnaire survey and anthropometric and biological measurements—was conducted between May and July 2016.ResultsIn the overall sample, 39.7% and 33.5% of the men and women had hypertension (systolic blood pressure (BP)≥140 or diastolic BP ≥90 mm Hg), respectively. Among the participants without a previous diagnosis of hypertension, 30.3% presented with hypertension at the time of measurement. In the multivariable analysis, alcohol intake and urban residence in men, and older age group, higher education and body mass index ≥25 kg/m2 in women were significantly associated with hypertension. Among the 21.8% who never had their BP measured, 83.8% were men; among these men, older age (adjusted OR (AOR), 0.43; 95% CI 0.25 to 0.73) and HIV positive status (AOR, 0.37; 95% CI 0.14 to 0.97) were negatively associated, while current smoker status (AOR, 2.09; 95% CI 1.19 to 3.66) was positively associated with the lack of BP measurements.ConclusionWe found that hypertension is prevalent in the target rural area. However, many were not aware of their hypertension status and many never had their BP measured, indicating a serious gap in cardiovascular disease prevention services in Zambia. There is an urgent need for health promotion and screening for hypertension, especially in the primary health services of rural Zambia. Issues related to healthcare accessibility in men require particular attention.
Mental health and health-related quality of life among healthcare workers in Indonesia during the COVID-19 pandemic: a cross-sectional study
Authors: Syamlan Adila T , Salamah Sovia , Alkaff Firas F , Prayudi Yogi E , Kamil Muhammad , Irzaldy Abyan , Karimah Azimatul , Postma Maarten J , Purba Fredrick Dermawan , Arifin Bustanul 
Published: April 2022

ObjectivesHealthcare workers (HCWs) are the front lines during the COVID-19 pandemic. They are more exposed to COVID-19 than other professions. Studies from other countries have shown that the mental health and health-related quality of life (HRQoL) of HCWs were affected during this pandemic. However, studies on mental health in Indonesia remain scarce and no study has evaluated the HRQoL among HCWs. Thus, this study was designed to explore the mental health status and HRQoL among HCWs in Indonesia.DesignThis was a cross-sectional study.SettingThis was an open online survey in Indonesia conducted from December 2020 to February 2021.ParticipantsThis study involved HCWs who worked during the COVID-19 pandemic. Of the 502 respondents who accessed the online questionnaire, 392 were included in the analysis.OutcomesMental health status was measured using the 21-item Depression, Anxiety and Stress Scale and HRQoL was measured using the second version of the 12-item Short-Form Health Survey (SF12v2).ResultsThe prevalence of depression, anxiety and stress among HCWs was 29.4%, 44.9% and 31.8%, respectively. Using the SF12v2 questionnaire, 354 (90.3%) HCWs were found to have impaired physical component and 156 (39.8%) HCWs have impaired mental component.ConclusionThe prevalence of mental health problems among HCWs was high in Indonesia. HRQoL, particularly the physical component, was affected in most HCWs. Thus, policymakers should give more attention to the mental health and HRQoL of HCWs during the COVID-19 pandemic.