bluuebunny/crossref_metadata_2025_split huggingface dataset:
Within or Without You? A Perspective Comparing In Situ and Ex Situ Tissue Engineering Strategies for Articular Cartilage Repair
Authors: O'Connell Cathal D. , Duchi Serena , Onofrillo Carmine , Caballero‐Aguilar Lilith M. , Trengove Anna , Doyle Stephanie E. , Zywicki Wiktor J. , Pirogova Elena , Di Bella Claudia 
Published: November 2022

AbstractHuman articular cartilage has a poor ability to self‐repair, meaning small injuries often lead to osteoarthritis, a painful and debilitating condition which is a major contributor to the global burden of disease. Existing clinical strategies generally do not regenerate hyaline type cartilage, motivating research toward tissue engineering solutions. Prospective cartilage tissue engineering therapies can be placed into two broad categories: i) Ex situ strategies, where cartilage tissue constructs are engineered in the lab prior to implantation and ii) in situ strategies, where cells and/or a bioscaffold are delivered to the defect site to stimulate chondral repair directly. While commonalities exist between these two approaches, the core point of distinction—whether chondrogenesis primarily occurs “within” or “without” (outside) the body—can dictate many aspects of the treatment. This difference influences decisions around cell selection, the biomaterials formulation and the surgical implantation procedure, the processes of tissue integration and maturation, as well as, the prospects for regulatory clearance and clinical translation. Here, ex situ and in situ cartilage engineering strategies are compared: Highlighting their respective challenges, opportunities, and prospects on their translational pathways toward long term human cartilage repair.
Where Microneedle Meets Biomarkers: Futuristic Application for Diagnosing and Monitoring Localized External Organ Diseases
Authors: Himawan Achmad , Vora Lalitkumar K. , Permana Andi Dian , Sudir Sumarheni , Nurdin Airin R. , Nislawati Ririn , Hasyim Rafikah , Scott Christopher J. , Donnelly Ryan F. 
Published: November 2022

AbstractExtracellular tissue fluids are interesting biomatrices that have recently attracted scientists' interest. Many significant biomarkers for localized external organ diseases have been isolated from this biofluid. In the diagnostic and disease monitoring context, measuring biochemical entities from the fluids surrounding the diseased tissues may give more important clinical value than measuring them at a systemic level. Despite all these facts, pushing tissue fluid‐based diagnosis and monitoring forward to clinical settings faces one major problem: its accessibility. Most extracellular tissue fluid, such as interstitial fluid (ISF), is abundant but hard to collect, and the currently available technologies are invasive and expensive. This is where novel microneedle technology can help tackle this significant obstacle. The ability of microneedle technology to minimally invasively access tissue fluid‐containing biomarkers will enable ISF and other tissue fluid utilization in the clinical diagnosis and monitoring of localized diseases. This review attempts to present the current pursuit of the application of microneedle systems as a diagnostic and monitoring platform, along with the recent progress of biomarker detection in diagnosing and monitoring localized external organ diseases. Then, the potential use of various microneedles in future clinical diagnostics and monitoring of localized diseases is discussed by presenting the currently studied cases.
Antitumor Activity of Anti‐miR‐21 Delivered through Lipid Nanoparticles
Authors: Zhang Zhongkun , Huang Yirui , Li Jing , Su Fei , Kuo Jimmy Chun‐Tien , Hu Yingwen , Zhao Xiaobin , Lee Robert J. 
Published: November 2022

AbstractThe ability of lipid nanoparticles (LNPs) to deliver nucleic acids have shown a great therapeutic potential to treat a variety of diseases. Here, an optimized formulation of QTsome lipid nanoparticles (QTPlus) is utilized to deliver an anti‐miR‐21 (AM21) against cancer. The miR‐21 downstream gene regulation and antitumor activity is evaluated using mouse and human cancer cells and macrophages. The antitumor activity of QTPlus encapsulating AM21 (QTPlus‐AM21) is further evaluated in combination with erlotinib and atezolizumab (ATZ). QTPlus‐AM21 demonstrates a superior miR‐21‐dependent gene regulation and eventually inhibits A549 non‐small cell lung cancer growth in vitro. QTPlus‐AM21 further induces chemo‐sensitization of A549 cells to erlotinib with a combination index of 0.6 in inhibiting A549 cell growth. When systemically administers to MC38 tumor‐bearing mouse model, QTPlus‐AM21 exhibits an antitumor immune response with over 80% tumor growth inhibition (TGI%) and over twofold and fourfold PD‐1 and PD‐L1 upregulation in tumors and spleens. The combination therapy of QTPlus‐AM21 and ATZ further shows a higher antitumor response (TGI% over 90%) and successfully increases M1 macrophages and CD8 T cells into TME. This study provides new insights into the antitumor mechanism of AM21 and shows great promise of QTPlus‐AM21 in combination with chemotherapies and immunotherapies.
Self‐Healing Electronics for Prognostic Monitoring of Methylated Circulating Tumor DNAs
Authors: Fang Peilin , Ji Xianglin , Zhao Xi , Yan‐Do Richard , Wan Youyang , Wang Ying , Zhang Yuanting , Shi Peng 
Published: November 2022

AbstractMethylated circulating DNAs (ctDNAs) have recently been reported as a promising biomarker for early cancer diagnostics, but limited tools are currently available for continuous and dynamic profiling of ctDNAs and their methylation levels, especially when such assays need to be conducted in point‐of‐care (POC) scenarios. Here, a self‐healing bioelectronic patch (iMethy) is developed that combines transdermal interstitial fluid (ISF) extraction and field effect transistor‐based (FET‐based) biosensing for dynamic monitoring of methylated ctDNAs as a prognostic approach for cancer risk management. The projection micro‐stereolithography‐based 3D patterning of an Eutectic Gallium‐Indium (EGaIn) circuit with an unprecedented 10 µm resolution enables the construction of self‐healing EGaIn microfluidic circuits that remain conductive under 100% strain and self‐healing under severe destruction. In combination with continuous transdermal ISF sampling of methylated ctDNAs, iMethy can detect ctDNAs as low as 10−16 m in cellular models and is capable of phenotypic analysis of tumor growth in rodent animals. As the first demonstration of a wearable device for real‐time in vivo analysis of disease‐indicative biomarkers, this proof‐of‐concept study well demonstrated the potential of the iMethy platform for cancer risk management based on dynamic transdermal surveillance of methylated ctDNAs via a painless and self‐administrable procedure.
Tension‐Induced Cavitation in Li‐Metal Stripping
Authors: Wang Chunyang , Lin Ruoqian , He Yubin , Zou Peichao , Kisslinger Kim , He Qi , Li Ju , Xin Huolin L. 
Published: November 2022

AbstractDesigning stable Li metal and supporting solid structures (SSS) is of fundamental importance in rechargeable Li‐metal batteries. Yet, the stripping kinetics of Li metal and its mechanical effect on the supporting solids (including solid electrolyte interface) remain mysterious to date. Here, through nanoscale in situ observations of a solid‐state Li‐metal battery in an electron microscope, two distinct cavitation‐mediated Li stripping modes controlled by the ratio of the SSS thickness (t) to the Li deposit's radius (r) are discovered. A quantitative criterion is established to understand the damage tolerance of SSS on the Li‐metal stripping pathways. For mechanically unstable SSS (t/r < 0.21), the stripping proceeds via tension‐induced multisite cavitation accompanied by severe SSS buckling and necking, ultimately leading to Li “trapping” or “dead Li” formation; for mechanically stable SSS (t/r > 0.21), the Li metal undergoes nearly planar stripping from the root via single cavitation, showing negligible buckling. This work proves the existence of an electronically conductive precursor film coated on the interior of solid electrolytes that however can be mechanically damaged, and it is of potential importance to the design of delicate Li‐metal supporting structures to high‐performance solid‐state Li‐metal batteries.
Advances in Materials and Technologies for Gas Sensing from Environmental and Food Monitoring to Breath Analysis
Authors: Milone Angelo , Monteduro Anna Grazia , Rizzato Silvia , Leo Angelo , Di Natale Corrado , Kim Sang Sub , Maruccio Giuseppe 
Published: November 2022

AbstractGas sensing research experiences a worldwide revival in the last years. From one side, the emergence of novel sensing materials enables unprecedented capacities for improving the device performances. From the other, the increasing opportunities for applications impacting current societal priorities highly motivate further studies. Herein, this field is reviewed with special attention to the emerging approaches and the most recent breakthroughs, challenges, and perspectives. In particular, this study focuses on: 1) the sensing layers analyzing recent trends toward nanostructured, low‐dimensional and composite materials; and 2) the latest achievements and targets in terms of applications, from environmental monitoring to food aroma identification and quality control up to the healthcare sector with breath analysis and diseases diagnosis.
Submicron Vertical Channel Organic Electrochemical Transistors with Ultrahigh Transconductance
Authors: Koutsouras Dimitrios A. , Torricelli Fabrizio , Blom Paul W.M. 
Published: November 2022

AbstractOrganic electrochemical transistors (OECTs) belong to the class of electrolyte gated organic transistors (EGOTs) that offer a smooth interface with biology in combination with high transconductance values of typically a few mS. Fabrication‐wise, though, most of the up‐to‐date reported devices are limited to two dimensional structures, where the transistor channel is patterned on the same plane as the source and drain electrodes. Here, a method is introduced for the fabrication of integrated vertical channel OECTs (vOECTs) with submicron channel length. By employing electrodeposition, a vertical channel sandwiched between the source and drain electrodes is created. Channel lengths down to 60 nm are demonstrated, giving rise to ultrahigh transconductance of up to 275 mS. Accounting for the voltage loss on the device connection tracks, an intrinsic transconductance of vOECTs of 500 mS is found. The vOECTs are three‐dimensional transistors finding relevant application in “organ‐on‐a‐chip” and implantable devices, where high amplification and small footprint are demanded.
Simulation of high‐concentration self‐interactions for monoclonal antibodies from well‐behaved to poorly‐behaved systems
Authors: Forder James K. , Ilott Andrew J. , Sahin Erinc , Roberts Christopher J. 
Published: November 2022

AbstractAttractive self‐interactions of therapeutic proteins are linked to problematic solution behaviors at high protein concentrations such as reversible or irreversible aggregation, high viscosity, opalescence, phase separation, and low solubility. Prediction of attractive self‐interactions early in development can improve the processes of formulation development and candidate selection. To that end, a coarse‐grained model with explicit representation of charged sites was used to accurately predict a broad range of protein self‐interactions at high protein concentrations (up to 160 mg/ml) for multiple monoclonal antibodies and formulations, including strong electrostatic attractions, with static light scattering measurements at low protein concentrations as the only experimental input. In addition, Mayer‐weighted electrostatic energies for charged residues from these simulations can contribute to understanding of electrostatic interactions and guide the development of protein variants.
Improved graph‐based multitask learning model with sparse sharing for quantitative structure–property relationship prediction of drug molecules
Authors: Hu Haoyang , Bai Yunke , Yuan Zhihong 
Published: November 2022

AbstractThe quantitative structure–property relationship (QSPR) is a fundamental technique for evaluating and screening potentially valuable molecules in the field of drug discovery. There is an urgent need to speed up pharmaceutical research and development and a huge chemical space to explore, which necessitate effective and precise computer‐aided QSPR modeling methods. Previous studies with various deep learning models are limited because they are trained on separate small datasets, known as the small‐sample problem. Using transfer learning, this article describes a sparse sharing method that uses advanced graph‐based models to construct an efficient and reasonable multitask learning workflow for QSPR prediction. The proposed workflow is systematically and comprehensively tested with four benchmark datasets containing different targets, and several precisely predicted molecular examples are illustrated. The results demonstrate that an obvious improvement in the prediction of molecular properties is achieved, along with the ability to predict multiple properties simultaneously.
A novel octamolybdate‐based organic–inorganic hybrid as photo‐Fenton‐like catalyst for degradation of methylene blue
Authors: Jiang Feng , Liu Qian‐Qian , Cui Zi‐Wei , Shi Shuang , Long Jian‐Ying , Wang Xiu‐Li , Fei Bao‐Li 
Published: November 2022

A γ‐Mo8O26‐based organic–inorganic hybrid [Cu2(L)2(γ‐Mo8O26)0.5(H2O)]•H2O (CuLMo8) [L = 5‐(pyrimidyl)tetrazolate] was hydrothermally synthesized and fully characterized by various spectral techniques. In the crystal structure of CuLMo8, the μ2‐bridging γ‐Mo8 anion coordinates with Cu ions to extend into a 2D network. CuLMo8 displayed excellent photo‐Fenton‐like catalyst properties for MB degradation under UV light irradiation. Complete decolorization of MB solution was finished in 5 min, and the degradation rate reached 97.9% in 7 min. The degradation rate of the present system was much higher than that of similar systems in the literature. The synergistic effect of CuLMo8 and H2O2 was the fundamental reason for the high degradation rate. Initial pH value and concentration of MB solution, catalyst dosage, and concentration of H2O2 were the main factors to affect the degradation rate. Hydroxyl (•OH) and superoxide (•O2H/O2•‐) radicals were the major reactive species to degrade MB molecules. Moreover, the total organic carbon (TOC) removal was about 37.5% in 3 h, indicating mineralization and decolorization did not take place at the same time. The possible MB degradation pathway was deduced depending on the data gained. In view of the high catalytic efficiency, repeated use, and no need to adjust the pH value of MB solution, this heterogeneous catalyst might have potential to deal with practical wastewater.
What is the ecological footprint of aquaculture after 5 decades of competition between mangrove conservation and shrimp farm development?
Authors: Ofori Samuel A. , Kodikara Sunanda K. A. , Jayatissa Loku P. , Madarasinghe Sanduni K. , Nijamdeen T. W. G. F. Mafaziya , Dahdouh‐Guebas Farid 
Published: November 2022

Abstract

Among the several threats to the conservation of mangrove ecosystems in most South Asian countries, shrimp farming is predominant. Since the introduction of shrimp farming in Sri Lanka in the 1980s, mangroves on the island’s north‐western coast have been continually cleared to create new shrimp farms, leading to a decline in the social‐ecological services provided by the mangrove ecosystems.
Using aerial (1973) and satellite (1996–2020) images, this study assessed areal changes in mangroves and shrimp farms in the Pambala‐Chilaw lagoon complex and Ihala Mahawewa, as well as the ecological footprint of shrimp farming in the study area.
Mangroves around the Chilaw lagoon had decreased in areal extent by 45% from 1973 to 2020 of which 92% of this change was attributed to shrimp farming. There was, however, a decrease in the areal extent of shrimp farms from 2001 to 2020, and a corresponding increase in mangroves from 2006 to 2020.
The ecological footprint of shrimp farming was assessed by comparing the expected surface ratios with those recorded for shrimp farms with mangroves and surface water bodies in the study area from 1973 to 2020. The results showed that the current shrimp farming was unsustainable (i.e. high ecological footprint).
While the results support the current view that there is cause for cautious optimism with mangrove conservation (as evidenced by an increase in mangrove areal extent), it also reveals that semi‐intensive shrimp farming in Sri Lanka and probably other similar tropical countries is unsustainable.
If immediate actions such as effective regulation of shrimp farming activities and mangrove restoration are not taken, the mangrove ecosystem will continue to decline.
A single‐cell surgery microfluidic device for transplanting tumor cytoplasm into dendritic cells without nuclei mixing
Authors: Okeyo Kennedy Omondi , Hiyaji Ryuta , Oana Hidehiro 
Published: November 2022

AbstractThis study aimed to demonstrate the feasibility of generating tumor cell vaccine models by single‐cell surgery in a microfluidic device that integrates one‐to‐one electrofusion, shear flow reseparation, and on‐device culture. The device was microfabricated from polydimethylsiloxane (PDMS) and consisted of microorifices (aperture size: ∼3 μm) for one‐to‐one fusion, and microcages for on‐device culture. Using the device, we could achieve one‐to‐one electrofusion of leukemic plasmacytoid dendritic cells (DC‐like cells) and Jurkat cells with a fusion efficiency of ∼ 80%. Fusion via the narrow microorifices allowed DC‐like cells to acquire cytoplasmic contents of the Jurkat cells while preventing nuclei mixing. After fusion, the DC‐like cells were selectively reseparated from the Jurkat cells by shear flow application to generate tumor nuclei‐free antigen‐recipient DC‐like (tarDC‐like) cells. When cultured as single cells on the device, these cells could survive under gentle medium perfusion with a median survival time of 11.5 h, although a few cells could survive longer than 36 h. Overall, this study demonstrates single‐cell surgery in a microfluidic device for potential generation of dendritic cell vaccines which are uncontaminated with tumor nucleic materials. We believe that this study will inspire the generation of safer tumor cell vaccines for cancer immunotherapy.
Financing solutions for circular business models: Exploring the role of business ecosystems and artificial intelligence
Authors: Fallahi Sara , Mellquist Ann‐Charlotte , Mogren Olof , Listo Zec Edvin , Algurén Peter , Hallquist Lukas 
Published: November 2022

AbstractThe circular economy promotes a transition away from linear modes of production and consumption to systems with circular material flows that can significantly improve resource productivity. However, transforming linear business models to circular business models posits a number of financial consequences for product companies as they need to secure more capital in a stock of products that will be rented out over time and therefore will encounter a slower, more volatile cash flow in the short term compared to linear direct sales of products. This paper discusses the role of financial actors in circular business ecosystems and alternative financing solutions when moving from product‐dominant business models to Product‐as‐a‐Service (PaaS) or function‐based business models. Furthermore, the paper demonstrates a solution where state‐of‐the‐art artificial intelligence (AI) modeling can be incorporated for financial risk assessment. We provide an open implementation and a thorough empirical evaluation of an AI‐model, which learns to predict residual value of stocks of used items. Furthermore, the paper highlights solutions, managerial implications, and potentials for financing circular business models, argues the importance of different forms of data in future business ecosystems, and offers recommendations for how AI can help mitigate some of the challenges businesses face as they transition to circular business models.
Chemoradiotherapy with paclitaxel liposome plus cisplatin for locally advanced esophageal squamous cell carcinoma: A retrospective analysis
Authors: Yi Qiong , Liu Canyu , Cui Yingshan , Yang Yanguang , Li Yaqi , Fan Xingwen , Wu Kailiang 
Published: November 2022

AbstractPurposeThis single‐center retrospective clinical study aimed to evaluate the efficacy and feasibility of chemoradiotherapy with paclitaxel liposome plus cisplatin for locally advanced esophageal squamous cell carcinoma (ESCC).MethodsPatients with locally advanced ESCC treated with paclitaxel‐liposome‐based chemoradiotherapy between 2016 and 2019 were retrospectively analyzed. Overall survival (OS) and progression‐free survival (PFS) were evaluated using Kaplan–Meier analysis.ResultsThirty‐nine patients with locally advanced ESCC were included in this study. The median follow‐up time was 31.5 months. The median OS time was 38.3 (95% confidence interval [CI]: 32.1–45.1) months, and the 1‐, 2‐, and 3‐year OS rates were 84.6%, 64.1%, and 56.2%, respectively. The median PFS time was 32.1 (95% CI: 25.4–39.0) months, and the 1‐, 2‐, and 3‐year PFS rates were 71.8%, 43.6%, and 43.6%, respectively. The most common Grade IV toxicity was neutropenia (30.8%) followed by lymphopenia (20.5%). There were no cases of Grade III/IV radiation pneumonia, and four patients (10.3%) had Grade III/IV esophagitis.ConclusionChemoradiotherapy using paclitaxel liposome and cisplatin is a well‐tolerated and effective treatment regimen for locally advanced ESCC.
Untargeted 1H‐NMR Metabolome of Celery During Fusarium Wilt: Implications for Vegetable Quality
Authors: Pacheco‐Hernández Yesenia , Hidalgo‐Martínez Diego , Zepeda‐Vallejo Gerardo , Cruz‐Narváez Yair , Escobar‐García Rosa Lilia , Becerra‐Martínez Elvia , Villa‐Ruano Nemesio 
Published: November 2022

AbstractCelery is a vegetable widely consumed as a condiment to prepare diverse dishes around the world. Nevertheless, this plant is susceptible to the attack of several phytopathogens including those of the Fusarium genus which is translated into devastating losses for the production chain. Herein we report on the metabolic changes produced during the celery wilt caused by Fusarium oxysporum which was determined through untargeted 1H‐NMR metabolomics. The changes in the metabolite content of celery were measured at 16, 24, and 32 days post‐inoculation using viable conidia obtained from the native F. oxysporum strain FO3. Our results demonstrated that the parasitic activity of the fungus reduced the endogenous levels of free sugars (fructose, galactose, glucose isomers, mannose, Myo‐inositol, mannitol, and sucrose) amino acids (alanine, aspartate GABA, glutamate, glutamine, histidine, isoleucine, leucine, methionine, proline, threonine, tyrosine, and valine), nucleosides (adenosine, cytidine, guanosine, and uridine) and organic acids (citric acid, fumaric acid, malic acid, and succinic acid). Interestingly, the levels of tyrosine and tryptophan were triggered as a consequence of F. oxysporum infection. This tendency was correlated with an increase in the levels of chlorogenic acid, apiin, and apigenin derivatives, suggesting their involvement in the chemical defense of celery against fungal colonization. According to principal component analysis (PCA) and Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS‐DA) methanol was the main differential metabolite and it was considered as a new chemical marker associated with F. oxysporum infection. Our results demonstrate that infected celery plants dramatically reduced their nutritional and nutraceutical contents during Fusarium wilt after 32 days post‐inoculation. However, these findings also suggest that the phenylpropanoid pathway is strongly related with the chemical defense of celery against F. oxysporum.