bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA methylation feature differences by gender in sarcopenia using data from the Korean Genome and Epidemiology Study
Authors: Lee Sangyeob , Gim Jeong-An , Kim Seung Chan , Baek Kyung-Wan , Yoo Jun-Il 
Published: October 2022

Abstract
Background: Sarcopenia is progressive loss of skeletal muscle mass and strength that can lead to physical impairment, poor quality of life, and death. DNA methylation is being studied as a hallmark with a crucial influence on aging and sarcopenia. However, studies have limitations in that they depended on a small sample size, and did not distinguish between those with sufficient muscle mass and those with insufficient muscle mass among the older people. Therefore, extensive studies on DNA methylation in older people with sarcopenia are needed.
Methods: We obtained Korean Genome and Epidemiology Study (KOGES) data conducted between 2009–2010 for analysis. We compared the demographic data of people with high muscle mass index (MMI) and those of people with low MMI. Furthermore, we conducted a DNA methylation study and investigated the effects of epigenetic factors on sarcopenia by identifying differentially methylated regions (DMRs). The pathfindR package of R software was used to perform DMR enrichment analysis to evaluate the relationship between identified DMRs and MMI according to gender.
Results: Muscle loss according to age was clearly revealed in men, but in women, the age difference according to MMI was not significant in demographic study. The enrichment analysis of DMRs showed that in the male group, human T-cell leukemia virus 1 infection showed the highest association, followed by allograft rejection, graft-versus-host disease, type 1 diabetes mellitus, and autoimmune thyroid disease. On the other hand, cell cycle showed the highest association, followed by ubiquitin-mediated proteolysis and the MAPK signaling pathway in women group. In men, many DMRs related to autoimmune were found, and in women, the ubiquitin-proteasome system-related DMRs played an important role.
Conclusions: The present study results provide differences according to gender in the epigenetic study of sarcopenia and provide an insight in the direction of further sarcopenia research.
Study the Activity of Natural Ligands of Lettuce as Agonists in the Management of Uremic Pruritus: An In-Silico Simulation Survey
Authors: Habibzadeh Sepideh , Ziari Mitra , Didehban Khadijeh 
Published: October 2022

Abstract
Uremic pruritus frequently occurs in patients on dialysis for more than three months. It is one of the most prominent, debilitating, and distressing symptoms in these patients, and despite various studies, no definite treatment has been found. The purpose of this research is to investigate the outstanding potential of natural components of lettuce (Lactuca Sativa L.) in the management of pruritus. ADME analysis and PASS predictions were employed to determine the drug likeliness potential of these natural ligands. A molecular docking study was performed on 19 bioactive ligands of lettuce against four protein receptors. The results showed that most of the selected compounds strongly react with kappa-opioid receptors 4DJH, 6VI4, 6B73, and G protein-coupled receptor 5ZTY and have better binding energies compared to the antipruritic drug gabapentin. The obtained docking scores (DS) and inhibition constants (Ki) revealed that the most potent antipruritic activities are exhibited by campesterol, δ-tocopherol, α-tocopherol, Δ-tocopherol, and α-lactucerol ligands. Our results from the MD simulation of the campesterol-5ZTY complex provide a theoretical validation that the ligand is stabilized in the protein active site. The binding free energy calculations quantitatively show that the association of the ligand to the protein increases the change in the total binding energy. This in-silico approach proposes that the natural ligands of lettuce could exert antipruritic effects by activating kappa-opioid receptors and may become recommended over synthetic drugs.
Anomalous retro-psoas iliac artery in a patient with Guillain-Barre syndrome: case report
Authors: Mouhsen Abdulmajeed , Rousan Liqa 
Published: October 2022

Abstract
The retro-psoas iliac artery is a rare vascular anomaly that has been reported few times in the literature; and while it may be clinically silent with no specific symptoms; reporting it can prevent many complications that may arise during surgical and interventional procedures. We report a case of a 40-year-old male who presented with progressive neurological symptoms, and was found to have a right retro-psoas iliac artery as an incidental finding on a lumbar spine MRI.
Bonding of isovalent homologous actinide and lanthanide pairs with chalcogenide donors: Effect of metal f-orbital participation and donor softness
Authors: Chattaraj Saparya , Bhattacharyya Arunasis 
Published: October 2022

AbstractChemistry of f-element gains importance in several fields due to the extensive scope of their applications. The aim of this study is to understand the subtle differences in bonding of theexceptionally similar actinides and their homologous (isovalent) lanthanides with several donors, which may lead to their covalency mediated separation. Several experimental and theoretical studies have been reported to address this aspect. However, to the best of our knowledge, systematic study on the variations in the bonding patterns of the isovalent ‘Ln’ and ‘An’ pairs encompassing the effect of valence f-orbitals participation was not encountered. In this study, the minute differences in covalent interactions of these isovalent f-element pairs with chalcogenide ions of varying softness via the metal (n-2)f orbital participation was probed using relativistic density functional theory (DFT). The f-electronic configurations of the metal ions were observed to play an important role in the f-orbital participation. f0, f7(half filled) [AnX]+/ [LnX]+pairs expresses resistance to f-orbital directed bonding, unlike the f3, f5, f6pairs. QTAIM study indicated that the f-orbital directed covalent bonding is pronounced for the [AnX]+systems of the latter set than the corresponding [LnX]+s, and it is near degeneracy driven. The extent of near degeneracy driven covalency was found to increase with the subsequent increase in the softness of the donor centres i.e. oxides through sulphides to selenides, although energetic stabilisation was not rendered. These results are anticipated to provide deeper understanding on exclusive differences in bonding of the homologous An3+/Ln3+ions with the chalcogenide donors.
Disposal of wood waste by granulation to produce biofuels
Authors: Malovanyy Myroslav , Paraniak Roman , Tymchuk Ivan , Zhuk Volodymyr , Ablіeіeva Iryna , Dziurakh Yurii 
Published: October 2022

Abstract
The perspective way of waste wood disposal by creation of pellet fuels has been considered. The prospects of using sulphate soap (a product of digestion of lignin from wood in pulp and paper production) as a binder for the creation of pellet fuels have been proved. Drying of waste woodto the required moisture is proposed to be carried out in an inviolable filtration layer. Optimal parameters of filtration drying and energy costs for the drying process are set for the conditions of the process implementation. The process of waste woodpelleting has been investigated and the optimal concentration of binder in the pellet has been established, which provides the necessary statistical strength and high calorific value of the pellet.
Assessment of Glucose-6 phosphate dehydrogenase deficiency in malaria suspected patients attending at Shele and Lante Health Center, Southern Ethiopia
Authors: Negash Meshesha T. , Feleke Sindew M. , Lo Eugenia , Nega Desalegn , Abebe Abnet , Dufera Boja , Kepple Daniel , Witherspoon Logan , Shenkutie Tassew T. , Adamu Aderaw , Gidey Bokretsion , Hailu Hiwot A. , Degu Sileshi , Kassie Enirsie , Mekonen Bacha , Yimer Mengistu , Golassa Lemu , Tassew Geremew , Dugassa Sisay 
Published: October 2022

Abstract
Background
In Ethiopia, an estimated 68 million peoples are at risk of malaria – 60% caused by Plasmodium falciparum and 40% by Plasmodium vivax. The national elimination program has begun a journey since 2016 with a vision to see a malaria-free country by 2030. The radical cure of P. vivax with the drug primaquine is an important component of the elimination strategy. However, Primaquine causes acute hemolytic anemia in glucose-6 phosphate dehydrogenase enzyme deficient cases and is a threat to P. vivax elimination. G6PD is a cytoplasmic enzyme for all human cells that involves in the pentose phosphate pathway of metabolic reaction and protects red blood cells from cellular oxidative damage by detoxifying free radicals. This study is therefore carried out to determine the G6PD deficiency prevalence among malaria-suspected patients in the study sites.
Method
Health facility-based cross-sectional study was conducted in 2021 at Shele and Lante health centers. A total of 858 self-presented malaria suspected patients were enrolled in this study. The socio-demographic and clinical information of the study participants were collected using a pre-validated questionnaire, entered into Epi Info™ 7 software, and analyzed using SPSS V.20 statistical software. Finger prick blood samples were collected for onsite CareSTART G6PD biosensor analyzer test, dried blood spot (DBS) preparation, and malaria microscopy. The DBS samples are used for molecular confirmation of G6PD deficiency.
Results
A total of 858 study participants of which 49.3% (423) were males with the median and interquartile age range of 26 and 21 years, respectively were enrolled in the study. Of all the study participants, 14.3%, 9.3%, and 4.1% were microscopy positive for P. falciparum, P. vivax, and mixed parasites, respectively. The phenotypic CareSTART biosensor analyzer G6PD deficiency rate was 4.8% (41/858). Whereas the molecular genotyping result analyzed in selected 13 patients have shown G6PD gene mutation in 10 (76.9%) of the samples. Particularly G267 + 119C/T mutations were seen in 9 of 13 (69.2%), A376G in 3/13 (23.1%), and G1116A in 3/13 (23.1%). In addition, new mutations such as A376T (A◊T) 2/13 (15.4%) and G1116T (G◊T 1/13 (77 %) were also identified.
Conclusion and recommendation
: The result implied that G6PD deficiency among the study participants is not significantly high. In addition, the G267 + 119C/T mutation was the most frequent variant reported in this study. Therefore, it is recommended to consider hemolysis risk while prescribing the primaquine drug in the study area.
The Role of Alcoholic Extract of Cranberry in Improving Serum Indices of Experimental Metaproterenol-induced Heart Damage in Rats
Authors: Salmasi Kimia , Hassanpour Ali , Tabrizi Bahram Amouoghli , Moghaddam Sina 
Published: October 2022

Abstract
Cranberry has countless cardiovascular benefits. According to some studies, this fruit causes the oxidation of low-capacity lipoprotein, improves high-capacity lipoprotein, reduces platelet coagulability, and promotes vascular activity. Albino male rats were divided into five groups (n = 5 per group). In the control group, normal saline was administered intraperitoneally. The second group was injected with metaproterenol (MET) three days a week for four weeks. The third, fourth, and fifth groups received 75, 100, and 150 doses of cranberry extract along with heart-damaging drugs. Then the blood samples were taken and sent to the laboratory on the fourth weekend and a week after finishing the injections in the fourth week (the sixth weekend) to check the serum factors, including cardiac creatine kinase MB (CK-MB), cardiac troponin I (cTnI), and aspartate aminotransferase (AST). The serum activity of the cardiac evaluation parameters in the fourth week revealed a highly significant relationship between the studied groups regarding AST and cTnI (p˂0.001). Moreover, a significant relationship was reported between AST and cTnI among the target groups (p˂0.05). Finally, the findings revealed that the consumption of blueberry extract with its effect on heart function could effectively modify the serum indicators associated with heart damage. The used extract was also effective; however, its effects were not stable. Accordingly, it is recommended to be used synergistically with other chemical and herbal medicines to achieve more prolonged effects.
Wheat Leaf Disease classification using modified ResNet50 Convolutional Neural Network model
Authors: A USHA RUBY , J George Chellin Chandran , N Chaithanya B , J Swasthika Jain T , Patil Renuka 
Published: October 2022

Abstract
Wheat leaf disease prevention and treatment requires a accurate and rapid classification of wheat leaf diseases and their extent. Using healthy wheat, leaf rust, crown and root rot, and wheat loose smut as research objects, this study proposes a deep learning-based technique for classifying the wheat leaf diseases. A collaborative generative adversarial network is used as an image imputation in the proposed methodology, allowing a generator and discriminator network to properly estimate the missing data in the dataset using the residual method. It is used to improve the feature extraction in wheat leaf images. The major contribution of this study is to use a pre-trained deep learning convolutional neural network architecture as a foundation to improve and construct an automated tool for wheat leaf disease image categorization. To classify wheat leaf diseases, a modification to ResNet50 is being suggested. The ′Conv′, ′Batch Normaliz′, and ′Activation Leaky Relu′ layers were added as part of this modification. These layers are inserted into the ResNet50 architecture for accurate feature extraction and discrimination. Extensive tests are carried out to evaluate the proposed model's performance on photos from a large wheat disease classification dataset. The suggested approach outperforms ResNet50, InceptionV3, and DenseNet, according to the experimental findings. The suggested method achieves the greatest identification accuracy of 98.44%. These discoveries might aid in the accurate detection and categorization of wheat leaf diseases.
The UBA5-GABARAP-PERK axis promotes cartilage degeneration in osteoarthritis by inhibiting autophagy and promoting endoplasmic reticulum stress
Authors: Wang Genchun , Sun Kai , Guo Zhou , Hou Liangcai , Zheng Zehang , Xu Jingting , Zhang Xiong , Ye Yaping , guo fengjing 
Published: October 2022

Abstract
purpose
Osteoarthritis (OA) is one of the most common causes of disability in the elderly. Ubiquitin-like modifier-activated enzyme 5 (UBA5) is a critical factor in preventing cellular autophagy and causing endoplasmic reticulum stress but has not been studied in OA. We aimed and explored the involvement of the UBA5-GABARAP-PERK axis in regulating cartilage matrix metabolism and apoptosis in osteoarthritis.
Methods
Oxidative stress was induced using IL-1β, which disrupted the homeostatic balance of cartilage. In in vivo and in vitro experiments, Western blot, qt-QPCR, scanning electron microscopy, immunofluorescence, and mCherry-GFP-LC3 plasmid were applied to observe OA-associated cartilage degeneration, ROS production, mitochondrial function, autophagic flux, endoplasmic reticulum stress and matrix after application of UBA5 selective inhibitor DKM2-93, knockdown or overexpression of UBA5 changes in metabolic indicators. UBA5 adeno-associated virus was injected into the cavity of mice, and a mouse OA model was induced by DMM surgery. Histological analysis of cartilage degeneration was performed using immunohistochemistry, Safranin-O staining, HE staining, Micro-CT, OARSI, and synovitis score.
Results
Expression of UBA5 was increased in chondrocytes receiving IL-1β intervention. Knockdown of UBA5 in vivo and in vitro inhibited OA-related chondrogenic degeneration, alleviated mitochondrial dysfunction, stimulated autophagy, inhibited endoplasmic reticulum stress, reduced catabolism, and increased anabolism. Overexpression of UBA5 also promotes oxidative stress and disrupts the molecular signature of healthy chondrocytes. Mechanistically, the destructive function of UBA5 may be attributed to the activation of the PERK/ATF4 signaling pathway. Through immunoprecipitation experiments, UBA5 was shown to inhibit autophagy by interacting with GABARAP to activate the PERK signaling pathway. Inhibition of PERK attenuated UBA5-induced osteoarthritis. Our findings suggest that Jun-B and C-Jun transcription factors may promote UBA5 expression and inhibition of UBA5 expression by in vivo application of adeno-associated virus, reduce chondrocyte death, attenuate cartilage degeneration, and promote subchondral bone remodeling.
Conclusions
This study revealed that UBA5 might regulate chondrocyte matrix catabolism and anabolism through the UBA5-GABARAP-PERK axis, suggesting a potential role for UBA5 in OA cartilage injury.
Accurate identification of locally aneuploid cells by incorporating cytogenetic information in single cell data analysis
Authors: Li Ziyi , Li Ruoxing , Ganan-Gomez Irene , Abbas Hussein , Colla Simona , Garcia-Manero Guillermo , Sun Wei 
Published: October 2022

Abstract
Single-cell RNA sequencing is becoming an increasingly common tool to investigate the cellular population and patients' outcomes in cancer research. However, due to the sparse data and the complex tumor microenvironment, it is challenging to identify neoplastic cells that play important roles in tumor growth and disease progression. This challenge is exaggerated in the research of blood cancer patients, from whom the neoplastic cells can be highly similar to normal cells. Here, we present partCNV/partCNVH, a statistical framework for rapid and accurate detection of aneuploid cells with local copy number deletion or amplification. Our method uses an expectation–maximization (EM) algorithm with mixtures of Poisson distributions while incorporating cytogenetic information to guide the classification (partCNV). When applicable, we further improve the accuracy by integrating a hidden Markov model for feature selection (partCNVH). We evaluate the performance of the proposed methods using extensive simulation studies and three scRNA-seq datasets from patients with blood cancers. In these applications, our proposed methods demonstrate favorable accuracy and more interpretable results compared with existing methods. In the real data analysis, our results identify many biological processes that have or have not been previously validated, implying multiple biological processes involved in the oncogenesis of myelodysplastic syndromes and acute myeloid leukemia.
Specific KIR-HLA genotypes predict the outcomes of refractory or recurrent primary central nervous system lymphoma
Authors: Lin Zhiguang , Xu Huiwen , Ma Jingjing , Ma Yan , Li Qing , Kang Hui , Zhang Mengxue , Chen Bobin 
Published: October 2022

Abstract
Purpose

An effective salvage regimen for the reinduction of remission is lacking for refractory or recurrent primary central nervous system lymphoma (r/r PCNSL). This study aimed to evaluate the efficacy and safety of high-dose cytarabine plus temozolomide in treating r/r PCNSL and to explore the associated prognostic factors.

Methods

A single-center retrospective cohort study was conducted to assess the efficacy and safety of high-dose cytarabine and temozolomide (AT) in r/r PCNSL patients. KIR and HLA genotyping was performed on peripheral blood samples from each patient.

Results

Thirty PCNSL patients receiving an AT regimen (cytarabine 3 g/m2 for 2 days combined with temozolomide 150 mg/m2 for 5 days) in our institution were analyzed. The median age was 65 years (range 25–79 years). A total of 43.4% of patients (13/30) achieved an overall response within a median follow-up of 16 months (95% confidence interval [CI]: 11–23 months). The median PFS and OS of the cohort were 1.75 months (95% CI: 1–4 months) and 19.5 months (95% CI: 11 months to not calculable), respectively. Patients harboring KIR3DL1/HLA-B genotypes predicting low affinity had a higher response rate (p = 0.042) and longer median PFS (3 months) than those with KIR3DL1/HLA-B genotypes predicting high affinity (1 month) (p = 0.0047). Cox regression analysis indicated that KIR/HLA-B genotypes were independently associated with PFS (p = 0.043). However, KIR/HLA-B genotypes had no impact on the OS of the cohort. The toxicity of AT treatment was mild and manageable.

Conclusion

The AT regimen was well tolerated, and patients with specific KIR-HLA genotypes may benefit from this regimen.
Trial registration number: ChiCTR2100054482
Date of registration: 2021-12-18
Registration status: prospective registration
Performance Evaluation of Matrix-Matrix Multiplication using Parallel Programming Models on CPU Platforms
Authors: Akoushideh Alireza , Shahbahrami Asadollah 
Published: October 2022

Abstract
Today’s hardware platforms have parallel processing capabilities and many parallel programming models have been developed. It is necessary to research an efficient implementation of compute-intensive applications using available platforms. Matrix-matrix multiplication is an important kernel used in many scientific and multimedia applications. This kernel is a compute-intensive program too. This paper aims to efficiently implement this kernel using different parallel programming models such as SIMD, OpenMP, and OpenCL. Our experiment results for different matrix sizes show that speedups up to 6.5x, 3.2x, 16.7x, and 32x are achieved for SIMD, OpenMP, hybrid OpenMP-SIMD, and OpenCL implementations over-optimized serial implementation, respectively.
Improving the productivity of malic acid by alleviating oxidative stress during Aspergillus niger fermentation
Authors: Wu Na , Xing Mingyan , Chen Yaru , Zhang Chi , Li Yingfeng , Song Ping , Xu Qing , Liu Hao , Huang He 
Published: October 2022

Abstract
Background
As an attractive platform chemical, malic acid has been commonly used in the food, feed and pharmaceutical field. Microbial fermentation of biobased sources to produce malic acid has attracted great attention, due to its sustainability and environment-friendly. However, most studies mainly focus on improving yield and ignore shortening fermentation time. A long fermentation period means high cost, and hinders the industrial applications of microbial fermentation. Stresses, especially oxidative stress generated during fermentation, inhibit microbial growth and production, and prolongs fermentation period. Previous studies have shown that polypeptides could effectively relieve stresses, but the underlying mechanisms were poorly understood.
Results
In this study, polypeptides (especially elastin peptide) addition improves the productivity of malic acid in A. niger, resulting in shortening of fermentation time from 120 to 108 h. Transcriptome and biochemical analyses demonstrated that both antioxidant enzyme-mediated oxidative stress defense system, such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and nonenzymatic antioxidant system, such as glutathione, were enhanced in the presence of elastin peptide, suggesting elastin peptide relieving oxidative stresses is involved in many pathways. In order to further investigate the relationship between oxidative stress defense and malic acid productivity, we overexpressed three enzymes (Sod1, CAT, Tps1) related to oxidation resistance in A. niger, respectively, and these resulting strains display varying degree of improvement in malic acid productivity. Especially, the strain overexpressing the Sod1 gene achieved a malate titer of 91.85 ± 2.58 g/L in 96 h, corresponding to a productivity of 0.96 g/L/h, which performs better than elastin peptide addition.
Conclusions
Our investigation provides an excellent reference for alleviating the stress of the fungal fermentation process and improving fermentation efficiency.