bluuebunny/crossref_metadata_2025_split huggingface dataset:
IL-27 promotes cardiac fibroblast activation and aggravates cardiac remodeling post myocardial infarction
Authors: Ma Xiaoxue , Meng Qingshu , Gong Shiyu , Shi Shanshan , Liang Xiaoting , Lin Fang , Gong Li , Liu Xuan , Li Yinzhen , Li Mimi , Wei Lu , Han Wei , Liu Zhongmin , Zhou Xiaohui 
Published: May 2022

Abstract
Excessively chronic inflammation and cardiac fibrosis post myocardial infarction(MI) cause progressive ventricular remodeling, which finally leads to heart failure. We previously found a high level of IL-27 in the heart and serum till day 14 post ischemia-reperfusion heart. However, whether IL-27 is involved in chronic inflammation-mediated ventricular remodeling remains unclear. In the present study, we found IL-27 expression levels in serum and damaged heart tissue were elevated and continued to day14 in mice after myocardial infarction. We also found MI triggered high IL-27 expression in cardiac macrophages. The increased expression of IL-27 in the serum is correlated with cardiac dysfunction and fibrosis after MI in mice. Furthermore, addition of IL-27 significantly activated the JAK/STAT signaling pathway in cardiac fibroblasts (CFs). Meanwhile, treatment with IL-27 promoted the proliferation and migration, as well as the differentiation of CFs to myofibroblasts and ECM production induced by angiotensin II (Ang II). Collectively, high level of IL-27 mainly produced by cardiac macrophages post MI promotes the collagen production of CFs and aggravates cardiac fibrosis.
Effect modification by NT-proBNP on the association between adiponectin and total mortality in EPIC–Heidelberg: A case-cohort study
Authors: Mukama Trasias , Johnson Theron , Kaaks Rudolf , Katzke Verena 
Published: May 2022

Abstract
BackgroundNT-proBNP has been hypothesized as a possible explanation for the paradoxical association between adiponectin and cardiovascular and all-cause mortality. We examined the heterogeneities by NT-proBNP, sex, BMI, smoking status, hypertension and diabetes status in the association between adiponectin and cardiovascular disease risk and mortality. MethodsWe used a case-cohort design nested within the EPIC-Heidelberg cohort, including 1,387 incident cases of myocardial infarction or stroke, 582 deaths from cardiovascular causes and 2,352 total deaths. We estimated hazard ratios for the association between 1SD increase in log-transformed adiponectin levels and cardiovascular disease risk, cardiovascular mortality and total mortality using Prentice-weighted Cox-proportional hazard models and assessed heterogeneity of the associations across strata of covariates. ResultsOverall, adiponectin was significantly associated with all-cause mortality [HR = 1.09, 95%CI: 1.03-1.16, p = 0.004], the association with cardiovascular mortality did not reach statistical significance [1.10 (0.99-1.37), p = 0.073] whereas there was no association with cardiovascular disease risk. There was significant heterogeneity by NT-proBNP in the association between adiponectin and all-cause mortality (phet = 0.019) such that significant increase in hazards of mortality were restricted to participants in the highest tertile of NT-proBNP. Among participants in the highest tertile NT-proBNP, adiponectin showed a dose-response relationship with total mortality such that; compared to participants in the lowest quintile, those in the third, fourth and fifth were at 1.22 (0.87-1.70), 1.50 (1.07-2.11), and 1.59 (1.15-2.21) higher hazards of mortality respectively. ConclusionsSignificant association between adiponectin and mortality was only observed in the context of high NT-proBNP. Our findings provide further support for hypothesis that NT-proBNP may explain the adiponectin paradox.
Gibberellin and abscisic acid transporters facilitate endodermal suberin formation in Arabidopsis
Authors: Binenbaum Jenia , Wulff Nikolai , Camut Lucie , Kiradjiev Kristian , Tal Iris , Vasuki Himabindu , Anfang Moran , Zhang Yuqin , Sakvarelidze-Achard Lali , Davière Jean-Michel , Ripper Dagmar , Bergua Esther Carrera , Manasherova Ekaterina , Lazary Shani , Novak Vlastimil , Crocoll Christoph , Weinstein Roy , Cohen Hagai , Ragni Laura , Aharoni Asaph , Band Leah , Achard Patrick , Nour-Eldin Hussam , Shani Eilon 
Published: May 2022

Abstract
The plant hormone gibberellin (GA) regulates multiple developmental processes. It accumulates in the root elongating endodermis, but how it moves into this cell file and the significance of this accumulation are unclear. Here, we identified a monophyletic clade of NPF transporters required for GA and abscisic acid (ABA) translocation. We demonstrate that NPF2.14 is a subcellular GA/ABA transporter, the first to be identified in plants, facilitating GA and ABA accumulation in the root endodermis to regulate suberization. Further, NPF2.12 and NPF2.13, closely related proteins, are plasma membrane-localized GA and ABA importers that facilitate shoot-to-root GA12 translocation, regulating endodermal hormone accumulation. This work reveal that GA promotes root suberization and that GA and ABA can act non-antagonistically. We demonstrated how a clade of transporters mediates hormone flow while utilizing defined cell-file-specific vacuolar storage at the phloem unloading zone, allowing a hormone slow-release mechanism required for suberin formation in the maturation zone.
Characterization of SARS-CoV-2 distribution and microbial succession in a clinical microbiology testing facility during the SARS-CoV-2 pandemic
Authors: Sah Govind Prasad , Kovalick Grace , Chopyk Jessica , Kuo Peiting , Huang Lina , Ghatbale Pooja , Das Promi , Realegeno Susan , Knight Rob , Gilbert Jack A. , Pride David T. 
Published: May 2022

Abstract
Background: The exchange of microbes between humans and the built environment is a bidirectional and dynamic process that has significant impact on health. Most studies exploring the microbiome of the built environment have been predicated on improving our understanding of pathogen emergence, persistence, and transmission. Previous studies have demonstrated that SARS-CoV-2 presence significantly correlates with the proportional abundance of specific bacteria on surfaces in the built environment. However, in these studies, SARS-CoV-2 originated from infected patients. Here we perform a similar assessment for a clinical microbiology lab while staff were handling SARS-CoV-2 infected swab samples for RT-qPCR analysis. The goal of this study was to understand the distribution and dynamics of microbial population on various surfaces within different sections of a clinical microbiology lab during a short period of 2020 Coronavirus disease (COVID-19) pandemic.Results: We sampled floors, benches and sinks in three sections of an active clinical microbiology lab (bacteriology, molecular microbiology, and COVID) over a 3-month period. Although floor samples harbored SARS-CoV-2, it was rarely identified on other surfaces, and bacterial diversity was significantly greater in floor samples than sinks and benches. The floor-associated microbiota comprised greater proportions of bacteria commonly associated with natural environments (e.g., soils), and benchtops harbored a greater proportion of human-associated microbes, including Staphylococcus and Streptococcus, which may have originated from either laboratory staff or clinical samples. Finally, we show that the microbial composition of these surfaces did not change over time and remained stable.Conclusion: Despite of finding viruses on the floors, no lab-acquired infections were reported during the study period, which suggests that lab safety protocols and sanitation practices were sufficient to prevent pathogen exposures. Like other BEs and health care facilities the most common bacteria colonizing the surfaces in a clinical microbiology lab primarily consist of bacterial taxa of environmental and human origin. Although our analysis here does not identify the source of infection in any given case, it does help to elucidate microbes that colonize these surfaces and have the potential to cause LAIs.
Effect of vertical preparation on fit of heat pressed zirconia reinforced lithium disilicate and monolithic zirconia three-unit fixed partial dentures: A comparative study
Authors: Elsherbini Mohammed , Al-Zordk Walid , Diaa Mohamed , Amin Rahma A , Sakrana Amal Abdelsamad 
Published: May 2022

Abstract
Background: The aim of this study was to evaluate the effect of horizontal and vertical tooth preparation on the internal and marginal fit of zirconia reinforced lithium silicate and monolithic zirconia fixed partial dentures.Methods: Forty ceramic fixed partial dentures were divided into four groups (n=10) according to ceramic type and finish line design. Group HL: Horizontal tooth preparation with heat pressed zirconia reinforced lithium disilicate. Group HZ: Horizontal tooth preparation with zirconia fixed partial denture. Group VL: Vertical tooth preparation with heat pressed zirconia reinforced lithium disilicate fixed partial denture. Group: VZ: Vertical tooth preparation with zirconia fixed partial denture. Micro-CT was used to evaluate the fit of each fixed partial denture. Data were collected and statistically analyzed.Results: Horizontal preparation showed a significant better fit than that of vertical preparation for both abutments (P˂.001). Zirconia showed significant better fit than heat pressed zirconia reinforced lithium disilicate for the posterior abutment. All the results were within internal and marginal fit requirements.Conclusions: Nevertheless, being within the clinically acceptable fit values, the vertical preparation can negatively affect the marginal gap and internal fit of fixed partial dentures at certain points. The marginal gap and internal fit of the ceramic fixed partial dentures can be affected by ceramic type and finish line design.
Exercise impacts positively on bone mineral density in human immunodeficiency virus seropositives: Do health professionals know?
Authors: Maduagwu Stanley M , Zizighi Samuel , Mohammad Usman A , Nwanne Chiamaka A , Ani Kenneth U , Ihegihu Ebere Y , Nwosu Ifeoma B , Okonkwo Arinze C , Umunnah Joe O , Okonkwo Uchenna P , Muonwe Chidile S 
Published: May 2022

Abstract
Background
Despite the concomitant negative effects of human immunodeficiency virus on bone mineral density in HIV-infected persons, studies on knowledge of effect of exercise on this health parameter in HIV population among health professionals seem unavailable. This study therefore examined knowledge of effect of exercise on BMD in HIV-infected persons among health professionals.
Methods
This cross sectional survey employed researcher designed, experts - validated questionnaire to enroll 434 respondents. The reliability coefficient “r” of the questionnaire was 0.75 with 92% response rate. Descriptive and inferential statistics were used to summarize and analyze the data respectively. The alpha value was set at 0.05.
Results
Age range and mean age in years of the respondents were 21–59 and 35.88 ± 9.09 respectively; age group of 30–39 years predominated (45.3%). Males were in preponderance (52.4%), and most (74.4%) were married; while majority (54.7%) were nurses. Substantial proportion (54.2%) had less than good knowledge on the subject examined in this study. Significant difference (p < 0.05; p = 0.015) in knowledge of effect of exercise on BMD in HIV population existed among the professions. Post hoc showed that knowledge of the physicians (p = 0.001) and laboratory scientists (p = 0.029) was significantly higher than that of the nurses. Physiotherapists’ knowledge on this subject compared to that of other health professionals was statistically insignificant (p > 0.05).
Conclusion
Knowledge of effect of exercise on BMD in HIV infected persons among health professionals was surprisingly below expectation, and therefore appalling.
Clinical Evaluation and Periapical Status of Crowns and Fixed Partial Dentures in a Selected Saudi Population: Retrospective Study
Authors: Albaqawi Ahmed H. , Aljanakh Mohammad D. , Alshammari Bayan N. , Alshammari Modhi A. , Alshammari Rawan H. , Alshammari Ghadeer D. , Alshammari Bodor Z. , AlShammari Rawan A. , Alturki Ruqayyah F. , Madfa Ahmed A. 
Published: May 2022

Abstract
Background: Patient satisfaction is a critical component of clinical success in patients undergoing restorations. As a result, the purpose of this study was to assess the clinical performance of crowns and multi-unit fixed prosthodontics in order to determine the frequency of problems associated with crowns and fixed restorations in a Saudi subpopulation using clinical and radiographic parameters. Methods: Three-hundred patients who received crowns and tooth-supported fixed partial dentures at dental polyclinics center in Hail region, Saudi Arabia between March 2021 and October 2021 were included in the study. The method of purposeful sampling was used. Clinical and radiographic examinations were used to identify patients who received crowns and FPDs at the dental center during the specified time. Patients with at least three-unit FPDs or a single crown with a natural contra-lateral tooth / teeth met the inclusion criteria. Six general dentists performed clinical and radiographic examinations. The criteria for evaluation the crowns and FPDs were technical and biological complications and satisfaction of the patients. Cross-tabulation is used to determine the frequency and percentage of the various parameters used in the current study. Results: The majority of the restoration had non-acceptable marginal integrity 249 (83%). Most of the restorations 181 (60.3%) had acceptable morphology. Almost all of the restorations 289 (96.3%) had un-satisfactory color. Visible periodontal depth equal or more than 5 mm were found in 53 (17.7%). Most of the fixed restorations were associated with gingival bleeding 240 (80%), while periapical lesion was found on 72 (24% teeth in all patients. The most patients 219 (73%) reported to be non-satisfied with their fixed restorations. Conclusions: The technical and biological complications noted in the current study were higher to other studies of FPDs. The majority of patients were dissatisfied with the restoration.
Predictive models in Extracorporeal Membrane Oxygenation (ECMO): a systematic review
Authors: Giordano Luca , Francavilla Andrea , Bottio Tomaso , Dell'Amore Andrea , Gregori Dario , Navalesi Paolo , Lorenzoni Giulia , Baldi Ileana 
Published: May 2022

Abstract
PurposeExtracorporeal Membrane Oxygenation (ECMO) has been increasingly used in the last years to provide hemodynamic and respiratory support in critically ill patients. In this scenario, prognostic scores remain essential to choose which patients should initiate ECMO.This systematic review aims to assess the current landscape and inform subsequent efforts in the development of risk prediction tools for ECMO. MethodsPubMed, CINAHL, Embase, MEDLINE and Scopus were consulted. Articles between Jan 2011 and Feb 2022, including adults undergoing ECMO reporting a developed and validated predictive model, were included. Data extraction aimed to capture study characteristics, risk model characteristics and model performance. The risk of bias was evaluated through the Prediction model Risk Of Bias Assessment Tool (PROBAST).ResultsTwenty-six prognostic scores for in-hospital mortality were identified. The most common candidate variables were age, lactate concentration, creatinine concentration, bilirubin concentration, and days in mechanical ventilation prior to ECMO. Five out of 16 venous-arterial (VA)-ECMO scores and 3 out of 9 veno-venous (VV)-ECMO scores had been validated externally. Additionally, one score was developed for both VA and VV populations. No score was judged at low risk of bias.ConclusionMost models have not been validated externally or are inadequately built and apply after ECMO initiation, failing to answer if ECMO should be initiated or not. It has yet to be determined whether and to what extent a new methodological perspective may enhance the performance of predictive models for ECMO, with the ultimate goal to implement a model that positively influences patient outcomes.
Snow microorganisms colonise Arctic soils following snow melt
Authors: Malard Lucie , Bergk-Pinto Benoit , Layton Rose , Vogel Timothy , Larose Catherine , Pearce David A 
Published: May 2022

Abstract
Arctic soils are subjected to microbial invasion from either airborne, marine or animal sources. However, in winter, Arctic soils are isolated from outside sources other than snow, which is the sole source of microorganisms. Successful colonisation of soil by snow microorganisms depends on the ability to survive and compete of both, the invading and resident community. Our study monitored snow and soil microbial communities throughout snow melt to investigate the colonisation process of Arctic soils. Microbial colonisation appears to have occurred as all the characteristics of successful colonisation were observed. The colonising microorganisms originating from the snow were already adapted to the local environmental conditions and were subsequently subjected to many similar conditions in the Arctic soil. Furthermore, competition-related genes (e.g., motility, chemotaxis, and virulence) increased in snow samples as the snow melted. Overall, one hundred potentially successful colonisers were identified in the soil and, thus, demonstrated the deposition and growth of snow microorganisms in soils during melt.
Decrease in population of swans (Cygnus spp.) due to the development of areas adjacent to the wintering site
Authors: Hong Suk-Hwan , Yeum Jung-Hun , Kim Si-Hwan , Park June-Log 
Published: May 2022

Abstract
The aim of the present study was to investigate the relationship between Cygnus spp. population trends and land-use change and artificial development activities in Nakdonggang estuary. Analysis of the 15-year-long population change revealed a sharp decline of Cygnus columbianus population in winter, which could be attributed to the Nakdonggang estuary becoming unfeasible as their wintering site. In contrast, there was no sharp decline in the Cygnus cygnus population, although a moderate decline was identified. Over the past 30 years, farmland activities decreased by approximately 10% in the areas adjacent to the protected area, resulted from the growth of the urbanized area. The rate of decrease of water body area was evident, identified by the urban development through reclamation. Although the abundance of food resources for Cygnus spp. can only be understood indirectly, changes in land use around the wintering site, where the Cygnus spp. can acquire food, are expected to be decisive in their choice of habitat. C. cygnus and C. columbianus, whose individual characteristics have not been specifically investigated, are generally known to share the same habitat; we could identify a distinct difference in terms of habitat sensitivity. With urban development occurring around the wintering site, C. columbianus was confirmed to be more sensitive to urbanization compared to C. cygnus in Nakdonggang estuary.
Carbon emissions and economic impact of EU’s embargoing Russian fossil fuels
Authors: Liu Lijing , Jiang Hong-Dian , Liang Qiao-Mei , Creutzig Felix , Liao Hua , Yao Yun-Fei , Qian Xiang-Yan , Ren Zhong-Yuan , Qing Jing , Cai Qiran , Edenhofer Ottmar , Wei Yi-Ming 
Published: May 2022

Abstract
The war in Ukraine lays bar EU’s dependence of fossil fuel imports from Russia. Here, we use a global computable equilibrium model, C3IAM/GEEPA, to estimate CO2 emission and GDP impact of embargoing fossil fuels from Russia. We find that embargoes induce more than 10% reduction of CO2 emissions in the EU, and slight increases of emissions in Russia, while both regions experience GDP losses (around 2% for the EU, and about 5% for Russia, ignoring the relative impact of other sanctions). Reacting to increasing energy prices with demand-side response inside the EU would increase CO2 emission savings, while turning GDP losses into gains. Implementing a partial embargo with tariffs largely compensates for lost government revenue.
WITHDRAWN: Neonicotinoid Analysis in Sunflower (Helianthus annuus) Honey of Tekirdag Province in Turkey
Authors: Ozdemir Nurullah , Muz Mustafa Necati 
Published: May 2022

Abstract
The widespread neonicotinoid use in agriculture causes environmental pollution, but its toxicity to mammals is more limited. Honeybees can carry pollutants to hives, considered bioindicators of environmental pollution. Forager bees returning neonicotinoids treated sunflower fields cause residue accumulation in the hives, which reason colony-level adverse effects. This study analyzes neonicotinoid residues in sunflower (Helianthus annuus) honey sampled by beekeepers from Tekirdağ province. Honey samples have been subjected to liquid-liquid extraction methods before high-pressure liquid chromatography-mass spectrometry (HPLC-MS/MS). No neonicotinoid residues were found above the maximum residue limit in the sunflower honey samples analyzed.
Performance Evaluation of Small-scale Irrigation Scheme: Case Study of Selamko Irrigation Scheme, Upper Bilue Nile - Ethiopia
Authors: Berihune Zigiybel F. , Moges Michael M. 
Published: May 2022

Abstract
The study was conducted to evaluate the performance of Selamko small scale irrigation scheme using internal and external performance indicators. The irrigation scheme command area was 63 ha and during the study season the irrigated area was 42ha. Over all activities in primary data collected included: field observation, discharge measurements in the canals, soil moisture before irrigation and after irrigation and depth of water applied at the field and interviewing beneficiary farmers. Secondary data were collected from secondary sources that were South Gondar Small Scale Irrigation coordination office, SGBoWR and WUA of the scheme. In order to evaluate the irrigation water, use efficiency of the scheme, nine farmer fields were selected from the scheme in relation to their location: at the head, middle and tail end water users. The internal process indicators which include conveyance efficiency and application efficiency were used to evaluate the performances of the scheme. From the analyses of internal performance indicators, the conveyance efficiencies were found to be 56.9% at the main canal, 57.09 at secondary canal 1 and 54.55% at secondary canal 2. The canals supply less water than the demand of the delivery points. And the application efficiencies were estimated to be 56.994%. The overall efficiency was 35.268%. The output per command area has to be calculated as 60198.14Birr/ha and 89738.1Birr/ha at 2016/17 and 2015/16 irrigation season respectively. The output per cropped area were estimated as 90297.62 Birr/ha and 99621.1 Birr/ha at 2016/17 and 2015/16 irrigation season respectively. The output per water consumed has to be determined as 17.66 Birr/m3 and 20.14 Birr/m3 at 2016/17 and 2015/16 irrigation season respectively. The water supply and irrigation supply were found as 1.71 and 1.47 respectively. From the analysis of physical performance of the scheme, during the study period, the irrigation ratio of the scheme was 66.67% and the sustainability of the scheme is 62.69% but in 2015/16 irrigation season, the irrigation ratio was 84.7% and sustainability of the scheme was 90.08%. During the study period the total effectivity of scheme infrastructure was 20.69%. It displayed that high system maintenance is required. Generally, the performance of the irrigation scheme is poor. Therefore, scheme monitoring, capacity building of the users and the water user associations, providing the flow control measurement structures, adequate operation and maintenance of the system are required to improve the irrigation scheme performance.