bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal dietary components in the development of gestational diabetes mellitus: a review of observational studies to timely promotion of health
Authors: Lambert Victoria , Muñoz Sonia Edith , Gil Carla , Roman Maria Dolores 
Published: June 2022

Abstract
There is ample evidence that considers diet an important factor in the prevention of gestational diabetes mellitus (GDM). The aim of this review is to synthesise the evidence between GDM and maternal dietary components. We performed a systematic bibliographic search in Medline, Latin American and Caribbean Health Sciences Literature (Lilacs) and the Latin American Nutrition Archive (ALAN) for regional and local literature, limiting the searches to observational studies published between 2016 and 2021. Search terms related to nutrients, foods, dietary patterns and the relationship to GDM risk were included. The review included 35 articles, 9 of which were located in América. The articles about maternal dietary components were as follows: 16 for nutrient intake, 4 for food intake, 2 combined nutrient and food analysis and 13 for dietary patterns. Iron, processed meat and a low carbohydrate diet were positively associated with GDM. Antioxidant nutrients, folic acid, fruits, vegetables, legumes and eggs were negatively associated with GDM. Generally, western dietary patterns increase GDM risk and prudent dietary patterns decrease the risk. The importance of a healthy diet early in life and during pregnancy needs to be addressed in clinical practice to anticipate the development of complications, enabling skilled nutrition professionals to act in a timely manner to promote healthy lifestyles.
Severity index for a national foodborne outbreak surveillance system
Authors: Simpson Ryan B. , Sanchez Emily , Sallade Lauren E. , Kulinkina Alexandra V. , Naumova Elena N. 
Published: June 2022

Abstract
Few studies have developed data-driven indices to describe and compare disease outbreak severity with respect to health burdens. We created a severity index using the National Outbreak Reporting System (NORS) database from 01 January 2009 through 31 December 2019. We identified 11 metrics related to case morbidity/mortality, outbreak duration, and their ratios, normalized natural log-transformed metric values, and generated index scores by averaging complete metrics per outbreak. We compared scores by contaminant, year, and calendar month using Dunn’s tests and tobit regression models. We found 2346 of 9407 outbreaks (24.93%) lacked contaminant information. All-contaminant median score was 0.28 [0.16, 0.43]; Norovirus and Sapovirus outbreaks had lower median scores (0.25-0.26 [0.15, 0.37]) than Salmonella, Shigella, Escherichia, and Campylobacter outbreaks (0.35-0.47 [0.28, 0.60]). High cases per outbreak duration contributed to increased viral scores while high hospital and healthcare visits per primary cases contributed to increased bacterial scores. All-contaminant severity decreased by ~16% across the study period with summertime seasonal peaks in June-September. Our results emphasize the importance of data quality, integrity, and completeness for data-driven index scores. This index can inform resource allocation strategies for monitoring enteric illness outbreaks and establish an early warning system for real-time surveillance of emerging outbreak threats.
Study on Landscape Performance Evaluation of Rural Microlandscapes in Southeast China
Authors: Shen Lan , Zhang Yikang , Yao Minfeng , Lan Siren 
Published: June 2022

Abstract
This paper discusses the evaluation system based on the Combined Weights-Technique for Order Preference by Similarity to Ideal Solution(CW-TOPSIS) method and evaluates and optimizes the landscape performance of rural microlandscapes in highly urbanized areas by taking rural areas in Jinjiang, Fujian Province, as an example. As a quantitative study on microlandscapes in rural areas, this study constructed evaluation indicators based on the U.S. landscape performance series and combined them with performance evaluation methods in other related fields to establish a landscape performance evaluation system suitable for rural microlandscapes in highly urbanized areas. Taking social benefit as an example, 25 microlandscape projects in Yinglin town, Jinjiang city, comprehensive rankings were based on the AHP-Entropy method and TOPSIS method. In addition, this paper also analyzes the qualitative results reflected in the quantitative data and proposes appropriate optimization schemes and improvement measures to further improve the living environment in rural areas.
The influence of a large build area on the microstructure and mechanical properties of PBF-LB Ti-6Al-4V alloy
Authors: Bagasol Axieh Joy , Kaschel Frederico R. , Ramchandran Saranarayanan , Mirihanage Wajira , Browne David , Dowling Denis P. 
Published: June 2022

Abstract
This study investigated the print homogeneity of Ti-6Al-4V alloy parts, when printed over a large build area of 250 Χ 250 Χ 170 mm3, using a production scale laser powder bed additive manufacturing system. The effect of part location across this large build area, was investigated based on printed part porosity, microstructure, hardness, and tensile properties. In addition, a Hot Isostatic Pressing (HIP) treatment was carried out on the as-built parts, to evaluate its impact on the material properties. A small increase in part porosity from 0.01 to 0.09 % was observed with increasing distance from the argon gas flow inlet, which was located on one side of the build plate, during printing. This effect, which was found to be independent of height from the build plate, is likely to be associated with enhanced levels of condensate or spatter residue, being deposited at distances, further from the gas flow. Despite small differences in porosity, no significant differences were obtained for microstructural features such as prior β grain, α lath thickness and phase fraction, over the entire build area. Due to this, mechanical performance such as hardness and tensile strengths were also found to be homogenous across the build area. Additionally, it was also observed based on the lattice constants, that partial in-situ decomposition of α' → α +β) phases occurred during printing. Post HIP treatment result showed a decrease of 7 and 6%, in the Yield Strength (YS) and Ultimate Tensile Strength (UTS) respectively, which was associated with a coarsening of α lath widths. The potential of the laser powder bed system for large area printing was successfully demonstrated based on the homogenous microstructure and mechanical properties of the Ti-6Al-4V alloy parts.
T3E: a tool for characterising the epigenetic profile of transposable elements using ChIP-seq data
Authors: Paz Michelle Almeida da , Taher Leila 
Published: June 2022

Abstract
BackgroundDespite the advent of Chromatin Immunoprecipitation Sequencing (ChIP-seq) having revolutionised our understanding of the mammalian genome’s regulatory landscape, many challenges remain. In particular, because of their repetitive nature, the sequencing reads derived from transposable elements (TEs) pose a real bioinformatics challenge, to the point that standard analysis pipelines typically ignore reads whose genomic origin cannot be unambiguously ascertained.ResultsWe show that discarding ambiguously mapping reads may lead to a systematic underestimation of the number of reads associated with young TE families/subfamilies. We also provide evidence suggesting that the strategy of randomly permuting the location of the read mappings (or the TEs) that is often used to compute the background for enrichment calculations at TE families/subfamilies can result in both false positive and negative enrichments. To address these problems, we present the Transposable Element Enrichment Estimator (T3E), a tool that makes use of ChIP-seq data to characterise the epigenetic profile of associated TE families/subfamilies. T3E weights the number of read mappings assigned to the individual TE copies of a family/subfamily by the overall number of genomic loci to which the corresponding reads map, and this is done at the single nucleotide level. In addition, T3E computes ChIP-seq enrichment relative to a background estimated based on the distribution of the read mappings in the input control DNA. We demonstrated the capabilities of T3E on 23 different ChIP-seq libraries. T3E identified enrichments that were consistent with previous studies. Furthermore, T3E detected context-specific enrichments that are likely to pinpoint unexplored TE families/subfamilies with individual TE copies that have been frequently exapted as cis-regulatory elements during the evolution of mammalian regulatory networks. ConclusionsT3E is a novel open-source computational tool (available for use at: https://github.com/michelleapaz/T3E) that overcomes some of the pitfalls associated with the analysis of ChIP-seq data arising from the repetitive mammalian genome and provides a framework to shed light on the epigenetics of entire TE families/subfamilies.
Chimpanzees, dung beetles and seed dispersal: implications for habitat conservation of the West African chimpanzee Pan troglodytes verus in Taï National Park, Côte d'Ivoire
Authors: KOFFI Kouadio Kan Sylvestre , DOSSO Kanvaly , BAROT Sebastien , WITTIG Roman M. , ARISTOPHANOUS Marios , MORETTO Philippe , KONE Inza , TIHO Seydou 
Published: June 2022

Abstract
Seed dispersal is a key stage in the life cycle of plants, thus in forest regeneration, especially in the tropics. Chimpanzees are known as important seed dispersers and dung beetles play critical roles in secondary dispersal thus increasing the chance for seed post-dispersal success. Hence, understanding secondary seed dispersal by dung beetles is critical to our understanding of forest dynamics. The aim of this study was to evaluate the effects of successive dispersal by chimpanzees and by dung beetles on the surface dispersal and burial of the seeds of four plant species: (Dacryodes klaineana (Pierre) H.J. Lam, Diospyros mannii Hiern, Pycnanthus angolensis (Welw.) Warb. and Uapaca guineensis Muell) at three nesting site in the Taï National Park. To investigate this, 28 seeds i.e. seven seeds per plant species (N = 75, average ± SD: 28.24 ± 3.06 seeds) were introduced into 85 g of chimpanzee faecal matter. This set was exposed on the forest floor for 48h following an average faecal radius of 5 cm (N = 40, average ± SD: 5 ± 1.06 cm). From the observations made, it emerges an essentially surface dispersion, ie 45.6% of the seeds dispersed against 6.3% of seeds buried. The highest rate of vertically dispersed seeds, ie 10.31%, and average depth of burial, ie 7.45 cm, were quantified in the southern nesting sites. This distribution of seeds would not only be linked to the time of deposition of the faecal matter, but also and above all to the size of the secondary disperser.
Engineered an accessory enzymatic system of cellulase for simultaneous saccharification and co-fermentation
Authors: Liu Han , Wang Xuxin , Liu Yanping , Kang Zhuoran , Lu Jiaqi , Ye Yutong , Wang Zhipeng , Zhuang Xinshu , Tian Shen 
Published: June 2022

Abstract
The enhanced hydrolysis of xylan-type hemicellulose is important to maximize ethanol production yield and substrate utilization rate in lignocellulose-based simultaneous saccharification and co-fermentation system. In this study, we conduct d-integration CRISPR Cas9 to achieve multicopy chromosomal integration with high efficiency of reductase-xylitol dehydrogenase pathway in Saccharomyces cerevisiae. Subsequently, we devise a CBP-enabling S. cerevisiae consortium, in which every engineered yeast strain could secrete or display different assembly component to be adaptive assembled on the surface of scaffoldin-displaying yeast cell for synergistic catalysis and co-fermentation from steam-exploded Pennisetum purpureum. Despite the accumulation of xylitol, the maximum ethanol titer of genetic engineered yeast strain reached 12.88 g/l with the cellulose conversion of 91.21% and hemicellulose conversion of 55.25% under 30 ºC after 96 h with the addition of commercial cellulase. The elaborated cellulosomal organization toward genetic engineering of an industrially important microorganism presents a designed approach for advanced lignocellulolytic potential and improved capability of biofuel processing.
Prolong the shelf-life and quality attributes of ready-to-eat pomegranate arils using PLA/ZnO nanoparticle/ Zataria multiflora essential oil nanocomposite film
Authors: Heydari-Majd Mojtaba , Monjazeb-Marvdashti Leila , Bratovcic Amra , Abdolshahi Anna , Shadan Mohammad Reza , Yazdani-Dehnavi Marzieh 
Published: June 2022

Abstract
The dried pomegranate (Punica granatum L.) arils have several health benefits and are in demand worldwide. Therefore, in the current study, the wrapping effects of Poly L-lactide (PLA) activated with zinc oxide nanoparticles (ZnO NPs, 1.5% w/w) and Zataria multiflora Boiss. essential oil (ZEO, 1 and 1.5% w/w) was studied on pomegranate arils' storage-life extension in cold storage. The arils were analyzed periodically for properties of physiochemical (weight loss, pH, total soluble solids, color measurement, antioxidant activity and sensory attributes) and microbial (total aerobic bacteria and total fungi counts). Results showed that the ZEO causes to decrease and ZnO causes to increase of pH during storage. Also, the active films were more effective than the control film in maintaining TTS and preventing weight loss of arils. The PLA/ZnO/1.5% ZEO sample had the highest phenolic content (∼520 mg/100 ml in juice) and antioxidant activity (∼90%) on day 15. Nevertheless, the effect of ZEO on the color properties of arils was more than ZnO, and 0.5 and 1.5% ZEO-PLA, showed the highest a* (55.14) and b* (31.30) with fresh arils, respectively. The microbial test results showed that ZEO was more effective in controlling bacterial growth than ZnO. Based on atomic absorption spectrophotometer measurement, minimal amounts (∼29 mg Kg− 1 sample) of Zn2 + ions released into arils were observed. Generally, the active PLA could be used as a safe and effective way to prolong the storage life of arils in refrigerated conditions.
Prediction model of total agricultural carbon emissions in Henan Province based on neural network and nonlinear surface fitting
Authors: Pan Yisha , Wang Chongyang , Yu Beichen , Wang Yidi , Yang Zhiwen , Ren Kangde 
Published: June 2022

Abstract
Controlling carbon dioxide emissions and taking the road of green development are inevitable choices for the sustainable development of the earth. The prediction of agricultural carbon emissions is of guiding significance to accelerate the pace of reducing carbon emissions and is beneficial to guide green technology innovation. Based on six influencing factors such as chemical fertilizer, pesticide and agricultural film, this paper uses neural network model and nonlinear surface fitting method to carry out regression and prediction of total carbon emissions, and the conclusions are as follows: 1) In terms of the return of the carbon emissions and forecast, the absolute value of multilayer perceptron model residual |δi|∈[0,0.03], the radial basis function model of the absolute value of residuals |δi|∈[0,0.19], the former has better effect than the latter, this is because, the former on the various factors affecting more fully utilized; 2) Set up the prediction model of nonlinear curved surface fitting based on the complex plane, and established the carbon emissions for influence factors in the complex plane expressions: Z=z1+jz2, and by calculation |Z| can be clearly predicted each year, under the influence of various factors, prediction of carbon emissions. 3) The correlation coefficients between the prediction results of the neural network and the nonlinear surface fitting prediction model and the original data exceed 0.9, indicating that the two methods are used in the regression and prediction of total carbon emissions.
Explosive Separation Properties of Double-hole Charge Structure
Authors: Wang Meng , Zhu Yu , Chen Gang , Su Hong , Xuan Haijun , He Zekan , Wan Zhao 
Published: June 2022

Abstract
In order to explore the critical charge diameter for explosive separation of titanium alloy plates under the structure of double-hole charge. In this paper, based on the SPH algorithm in the AUTODYN software, a numerical model of the explosion separation of titanium alloy plates was established, and numerical simulations were carried out for different charge aperture models.According to the strength criterion and the simulation calculation results, the fracture judgment was carried out, and the fracture mechanism of the double opening separation was analyzed, and the law of the damage to the plate by the diameter of the charging hole was explored. According to the simulation results, the results show that the test results are highly consistent with the simulation results. When the double hole charge structure is used for explosive separation, the size of the charge aperture not only directly affects the effect of explosive separation, but also affects the flying off kinetic energy after plate fracture. Under the influence of various factors, the plate breaks from the hole wall from inside to outside, and finally breaks into a seam to complete the separation. With the increase of the charge hole diameter, the damage condition of the titanium alloy plate increases almost linearly, that is, the larger the charge hole diameter, the more serious the damage to the titanium alloy plate.
The Utility of Intraoperative Endoscopy to Assist Novice Surgeons in the Detection of Gastric Stenosis during Laparoscopic Sleeve Gastrectomy
Authors: Chen I-Sung , Tsai Ming-Shian , Chen Jian-Han , Chen Chung-Yen , Chen I-Lin , Tai Chi-Ming 
Published: June 2022

Abstract
Laparoscopic sleeve gastrectomy (LSG) is a commonly performed bariatric surgery, but is associated with gastric stenosis and leaks. Herein, we report our experience of intraoperative endoscopy (IOE) to evaluate stenosis and leaks during LSG. LSG was performed by three surgeons. Patients who underwent LSG and IOE between January 2016 and March 2020 were enrolled and assigned to two groups: group 1 (1st-30th LSG case for each surgeon) and group 2 (>30th LSG for each surgeon). Patients’ anthropometric and biochemical data pre- and post-LSG, as well as IOE findings and follow-up esophagogastroduodenoscopy records were reviewed. In total, 352 patients were enrolled including 90 patients in group 1 and 262 patients in group 2. Three out of 352 patients (0.9%) were found to have stenosis by IOE, which was related to tightly gastropexy stitch or reinforcement stitch, all of which were in group 1. Stenosis was resolved after removal of the stitch during LSG. The incidence of gastric stenosis detected by IOE was 3.3% (3/90) and 0% (0/262) in group 1 and group 2, respectively (P=0.003). No leakage was found in this study and no patient developed clinical or endoscopic stenosis after LSG. In conclusion, IOE can help detect gastric stenosis during LSG, especially for novice surgeons, and the stenosis could be resolved during operation.
Prefeasibility analysis of low-scale biorefineries: annatto and açai case
Authors: Poveda-Giraldo Jhonny Alejandro , Piedrahita-Rodríguez Sara , Salgado-Aristizabal Natalia , Moreno Haminton Salas , Alzate Carlos Ariel Cardona 
Published: June 2022

Abstract
The industrial applicability of exotic and wild fruits has been investigated as stand-alone processes framed to obtain a single main product. However, their combination and the valorization of the processing residues in a biorefinery scheme have not been explored. Therefore, this work aims to assess the technical and economic feasibility of low-scale biorefineries based on annatto and açai as raw materials. Therefore, the actual processing of both fruits was carried out experimentally. For annatto, the extraction of dye paste was performed, obtaining mass yields of up to 58.4% by working with ground seed. Regarding the açai, pulp was obtained with a polyphenol content of 23.56 mg of gallic acid equivalent per gram of sample. The residues generated in both processes were used as substrates for biogas production, achieving biomethane yields between 186–533 mL per gram of volatile solids. These results were the basis for the design and simulation of process scale-up in low-scale biorefinery contexts. Five scenarios were evaluated, involving the production of annatto dye paste, açai pulp, biogas from exhausted annatto seeds, slurry and seeds of açai, and feedstock integration. As the main energy result, it was found that the individual production of açai pulp can fully supply the required energy of the process through the self-generation index. From the economic perspective, it was found that there is economic feasibility working with flows below the regional and national production in Colombia for all scenarios.
Identification of N7-methylguanosine-related patterns and characterization of the tumor microenvironment in hepatocellular carcinoma
Authors: Yu Jianfa , Wang Shuang , Shi Rui , Yang Long , Lang Qi , Zhang Yamin 
Published: June 2022

Abstract
Background: N7-methylguanosine (m7G) is an essential epigenetic modification and there is increasing evidence that it is closely associated with tumorigenesis progression. However, the expression pattern of m7G-related genes in hepatocellular carcinoma (HCC) remains systematically analyzed.Methods: We downloaded HCC genetic and transcriptomic data from TCGA and GEO datasets. Unsupervised clustering methods identified different m7G-related gene subtypes, and then an m7G_score for predicting overall survival and characterizing the tumor microenvironment (TME) was constructed. Eventually, a highly accurate nomogram was constructed based on the score.Results: In this study, two m7G-related gene subtypes were obtained by unsupervised clustering analysis of 18 m7G-related genes, and the relationship between different subtypes and clinicopathological features and TME was systematically evaluated.Conclusion: N7-methylguanosine-related patterns are closely related to the clinical characteristics and tumor microenvironment of HCC patients, providing a new perspective on the treatment of HCC patients. Meanwhile, m7G_score can improve the clinical applicability with HCC patients.