bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enzymatic Simulation by (NAMD), Molecular Docking, Mutation, Structural and Thermodynamic Analysis of Xylanase (Xylcm8) Obtained from Camel Rumen
Authors: Rajabi Marzieh , Nasrollahi Narges , Rajabi Marjan , Noghabi Kambiz Akbari , Zahiri Hossein Shahbani 
Published: February 2022

Abstract
Xylanase is one of the essential enzymes in divergent industries such as Pulp bleaching, cosmetics, and beverages. It has been widely used in food and pharmaceutical industries and obtained from bacteria, fungi, and crustaceans that is mainly responsible for the hydrolysis of β-1,4 bonds in xylan polysaccharides. Structural and bioinformatics studies of xylanase, can be a contributory and effective aspect of enzyme metabolism and function in polysaccharide components hydrolases. Multiple methods and bioinformatical tools have been used in this study to achieve the whole enzyme structure. The exact enzyme location in the membrane and the main amino acid in the catalytic site have been obtained using computational approaches. This study covers some subjects as enzyme modeling, simulation, structural alignment with similar enzymes, and even docking analysis.
A Prospective Study of Risk Factors for Taxane-induced Peripheral Neuropathy in Patients With Breast Cancer
Authors: Liang Cuilv , Zhang Yin , chen qiyin , chen wenfa , chen mingzhu 
Published: February 2022

Abstract
Purpose: The study investigated the clinical risk factors of peripheral neuropathy induced by docetaxel and albumin-bound paclitaxel (AP) in breast cancer patients. Methods: A prospective observational study was performed in 268 patients from March 2019 to December 2020, related information of patients can be obtained by querying the electronic system, consulting the patient and evaluating the scale, neuropathic symptoms are followed up from the beginning to three months after taxane drug chemotherapy. Univariate and multivariate analysis were used to find the risk factors of overall and severe taxane-induced peripheral neuropathy (TIPN).Results: The cumulative dose (OR=3.533, 95% CI: 1.797～6.944，p<0.001) , body mass index (BMI)(OR=2.926，95% CI: 1.621～5.281，p<0.001), body surface area(BSA) (OR=1.724，95% CI: 1.011～2.941, p=0.045) , hypocalcemia (OR = 4.899, 95% CI: 1.518 ~ 15.811, P = 0.008) all increased the risk of TIPN, only cumulative dose (OR=2.577，95% CI: 1.161～5.719，p=0.020) and BSA (OR=2.040，95% CI: 1.073～3.877，p=0.030) were independent risk factors for severe TIPN.Conclusion:Cumulative dose, BMI, BSA, and hypocalcemia are risk factors for overall TIPN, while cumulative dose and BSA are risk factors for severe TIPN. Breast cancer patients, those with high BMI and large BSA, hypocalcemia and large cumulative dose can be closely followed by TIPN, preventive intervention measures actively carried out.
Trophic Structure and Origin of Resources of Soil Macrofauna in the Salt Marsh of the Wadden Sea: A Stable Isotope (15N,13C) Study
Authors: Rinke Maria , Bendisch Philipp M. , Maraun Mark , Scheu Stefan 
Published: February 2022

Abstract
Salt marshes exist along the gradient of the marine mudflat to the terrestrial dunes, with a gradient of shore height and associated plant zonation. The lower salt marsh (LSM) extends from the mean high tidal level to 35 cm above that level and is followed by the upper salt marsh (USM). Despite changes in the amount of allochthonous marine input and in abiotic conditions, little is known about changes in the trophic structure and used of basal resources by the soil macrofauna along marine – terrestrial boundaries. Natural variations in carbon stable isotope ratios (δ¹³C signatures) allow insight into basal resources of consumers such as marine algae, terrestrial C3 and C4 photosynthesising plants. Furthermore, variations in nitrogen stable isotope ratios (δ¹5N signatures) allow insight into the trophic position of consumers. We investigated spatial and temporal changes in stable isotope signatures in salt marsh soil macrofauna of the island of Spiekeroog. The range of δ¹⁵N signatures indicated no changes in food chain length across salt marsh zones with consumers in both zones comprising primary decomposer, secondary decomposer and first order predators. However, the trophic position of individual species changed between zones, but in particular with season. Contrasting δ¹⁵N signatures, the range in δ¹³C signatures in the LSM was twice that in the USM indicating a wider range of resources consumed. Bayesian mixing models indicated predominant autochthonous resource use in both the LSM and USM, with allochthonous resource use never exceeding 29.6%. However, the models also indicate an increase in the use of marine resources in certain species in the LSM with no use in the USM. Overall, the results indicate that the resource use of salt marsh macrofauna varies more in time than in space, with the food web being generally based on autochthonous rather than allochthonous resources. However, there also is trophic plasticity in certain species across both temporal and spatial scales including variations in the use of allochthonous resources. Generally, however, marine input contributes little to the nutrition of salt marsh soil macroinvertebrates.
Physical activity and chronic obstructive pulmonary disease: a scoping review
Authors: 项新月  , 黄丽华  , 方勇  , 蔡莎莎  , 张明月  
Published: February 2022

Abstract

Background
Reduced physical activity (PA) was the strongest predictor of all-cause mortality in patients with chronic obstructive pulmonary disease (COPD). This scoping review aimed to map the evidence on the current landscape of physical activity, barriers and facilitators, and assessment tools across COPD patients.
Methods
Arksey and O'Malley's scoping review methodology framework guided the conduct of this review. An electronic search was conducted on five English databases (PubMed, Cochrane library, PsycINFO, CINAHL and Web of Science (Medline)) and three Chinese databases (CNKI, CQVIP and WAN-FANG) in November 2021. Two authors independently screened the literature, extracted the studies characteristics.
Results
The initial search yielded 3686 results, of which 1754 were duplicates. Of the remaining 135 articles, 42 studies met the inclusion criteria. Among the reviewed articles, there were 14 (33.3%) cross-sectional study, 9 (21.4%) cohort study, 4 (9.5%) longitudinal study, 3 qualitative study, 12 (28.7%) randomized control trials (RCTs). The main barriers identified were older age, women, lung function, comorbidities, COPD symptoms (fear of breathlessness, severe fatigue, anxiety and depression), GOLD stage, frequency of exacerbation, oxygen use, low motivation and environment-related (season and weather). Twelve studies have evaluated the effects of physical exercise (e.g., walking training), pulmonary rehabilitation, pedometer, self-efficacy enhancing intervention, behavioral modification intervention on physical activity and have had inconsistent results.
Conclusions
Changing physical activity behavior in patients with COPD requires multidisciplinary collaboration. Future studies need to identify the best instruments to measure physical activity in clinical practice. Future studies should focus on the effects of different types, time and intensity of physical activity in COPD patients and conduct randomized, adequately-powered, controlled trials to evaluate the long-term effectiveness of behavioral change interventions in physical activity.
Wear Evaluation of Poly(vinyl alcohol) Hydrogel by UV Spectrometry and TOC Measurement of Lubricant
Authors: Takefuji Haruna , Annaka Masahiko , Yashima Shintaro 
Published: February 2022

Abstract
We report the wear evaluation of poly(vinyl alcohol) (PVA) hydrogel by measuring the polymer concentration in the lubricant after the sliding test. Hydrogels are expected as biomaterials, and wear evaluation is required for their application. However, the swelling and drying behavior of hydrogels makes accurate wear evaluation difficult by applying the same method for solid materials. In this work, sliding tests were conducted, then the microscopic observation of the worn gel sample and the quantitative wear evaluation using the lubricant were performed. To understand the relationship between wear and surface geometry, glass substrates with various surface roughness and hydrogels with different surface geometries were used. UV spectrometry and total organic carbon measurements of the lubricant revealed that the wear amount increases with sliding time or surface roughness of the substrate, and it is possible to quantitatively evaluate the wear of the PVA gel. The effect of surface dimples on the hydrogel was different depending on the counter substance; friction and wear decreased against the glass, but low friction and high wear appeared against the gel. Because there were a few wear scars on the gel with dimples, it was suggested that dimples scrape and trap the generated wear particles, reducing the transfer of wear particles and surface damage. Measuring the concentration of PVA polymers in the lubricant made it possible to separately and quantitatively evaluate whether the wear of the gel was due to surface deformation and transfer, or loss due to surface damage.
A comparison of opioid-containing anesthesia versus opioid-free anesthesia using the Cortínez-Sepúlveda model on differential cytokine responses in patients undergoing gastric bypass surgery: a randomized controlled trial
Authors: Martínez-López Erika , Campos-Pérez Wendy , Ramírez-Plascencia Lilia , Pérez-Robles Mariana , Rivera-Valdés Juan J , Sánchez-Muñoz Patricia , Pérez-Vargas Liliana , González-Landeros Dulce , Cuevas Juan Heberto Muñoz 
Published: February 2022

Abstract
Background: Anesthetic agents, including opioids can modulate the altered immune function in patients with obesity through mechanisms that involve the expression and release of cytokines. For this reason, anesthetic care in patients with obesity remains controversial. Therefore, the aim of the study was to compare the effect of opioid-containing anesthesia vs opioid-free anesthesia using the Cortínez-Sepúlveda model on serum levels of IL-6, IL-1β and TNF-α before and after surgery in obese patients undergoing bypass surgery.Methods: A randomized cross-sectional study of 40 unrelated obese adults was performed at the Civil Hospital of Guadalajara “Dr. Juan I. Menchaca”. Before undergoing laparoscopic Roux-en-Y gastric bypass, patients were randomly assigned to two anesthesia groups: opiod-containing (n=20) or opiod-free (n=20). The opioid used in the opioid-containing anesthesia group was fentanyl. To characterize the disposition of intravenous propofol for the target-controlled infusion technique in obese patients, the Cortínez-Sepúlveda pharmacokinetic model was used. Body mass was determined to the nearest 0.05kg using a balance scale (Seca 703; Seca, Hamburg, Germany). Blood samples were taken before and immediately after surgery and cytokine serum levels were determined with ELISA kits. Pain was assessed using a numerical pain rating scale. Adverse effects were collected 24 h after surgery.Results: 6 men and 34 women were included in this study (37.9±10.6 years). Pre-surgery IL-6 and TNF-α serum levels were not detected in all participants. However, IL-1β levels significantly decreased after surgery (49.58pg/mL (18.50-112.20)-before surgery vs 13pg/mL (5.43-22)-after surgery, p=0.019). IL-6 concentrations were significantly higher in subjects who received opioid-containing anesthesia (with fentanyl) compared to subjects with opioid-free anesthesia (224.5pg/mL (186.3-262.8) vs 99.5pg/mL (60.8-138.2), respectively, p<0.001; adjusted by age, gender, and BMI). In addition, the use of opioids confers an increased risk for higher IL-6 levels in obese patients (OR=2.95, 95% CI: 1.2-7.2, p=0.010). A linear regression model showed that the operative time (in hours) of bypass surgery and anesthetic technique were positively correlated with IL-6 levels.Conclusion: Anesthesia with opioids was positively related to IL-6 serum levels in obese patients undergoing bypass surgery. This finding could have clinical relevance when choosing an appropriate anesthetic management plan for bariatric surgical patients. Trial registration: The study was retrospectively registered at ClinicalTrials.gov Identification Number: NCT04854252, date 22/04/2021.
The End Of Life Experiences Of People Living With Socio-Economic Deprivation In The Developed World: An Integrative Review
Authors: Bowers Sarah P , Chin Ming , O'Riordan Maire , Carduff Emma 
Published: February 2022

Abstract
Background: Those experiencing socioeconomic deprivation have poorer quality of health throughout their life course which can result in poorer quality of death – with decreased access to palliative care services, greater use of acute care, and a disparity in deaths at home compared with patients from less deprived populations.Aim: To summarise the current global evidence from developed countries on end-of-life experience for those living with socio-economic deprivation.Design: Integrative review in accordance with PRISMA. A thorough search of major databases from 2010-2020, using clear definitions of end-of-life care and well-established proxy indicators of socio-economic deprivation. Empirical research describing experience of adult patients in the last year of life care were included.Results: Forty studies were included from a total of 3508 after screening and selection. These were deemed to be of high quality; from a wide range of countries with varying healthcare systems; and encompassed all palliative care settings for patients with malignant and non-malignant diagnoses. Three themes were identified: 1) the multi-dimensional experience at end-of-life, 2) preferences and planning and 3) the uptake and utilization of services.Conclusions: Current models of healthcare services are not meeting the needs of those experiencing socio-economic deprivation at the end-of-life. Further work is needed to understand the disparity in care, particularly around ensuring patients voices are heard and can influence service development and delivery.
Live with mask and guilt: A qualitative study on experiences of stigma among postoperative oral cancer patients in China
Authors: Tan Chuxia , Yang Ronghong , Wang Dangdang , Zhong Chenxi , Mei Ranran , Deng Xianjiao , Chen Shihao , Li Wei , Ye Man 
Published: February 2022

Abstract
Objectives: To explore the drives, manifestations and coping strategies of stigma in postoperative oral cancer patients to provide a reference for the formulation of targeted intervention measures.Methods: Using a phenomenological approach, we enrolled 15 postoperative oral cancer patients in a certain Grade a hospital from 10 March 2021 to 30 June 2021 in Hunan Province, China. Semi-structured interviews were conducted. The interview data were analyzed using a framework analysis based on qualitative content analysis methods. This study aligns with the COREQ checklist.Results: The stigma experience of postoperative oral cancer patients can be divided into 3 themes: (1) drivers (internal factors and external factors); (2) manifestations (isolation, feeling of guilt, feeling of inferiority and the experience of discrimination); (3) coping strategies (self-adjustment, seeking social support and enhance physical quality).Conclusion: Postoperative oral cancer patients lived with mask and guilt, and experienced multiple forms of discrimination. Further work is needed to increase education and awareness about oral cancer to guide them to take positive coping and reduce stigma.
Microseismic energy distribution and impact risk analysis of complex heterogeneous spatial evolution of extra-thick layered strata
Authors: Lai Xingping , Jia Chong , Cui Feng , Chen Jianqiang , Zhou Yupu , Feng Ganggui , Gao Yuanjiang 
Published: February 2022

Abstract
In the process of deep mining of coal resources, coal seams with better geological conditions are gradually mined preferentially, and the problem of safe and efficient mining of working faces in complex and heterogeneous spaces of residual coal seams is faced. Taking the Kuangou Coal Mine as the background, using microseismic monitoring instruments and pressure sensor monitoring systems, the rock pressure appearance and microseismic energy characteristics accompanying the evolution of the overburden strata structure in the mining of solid coal and the lower working face of the gob are analyzed. Research on the precursory characteristics and early warning of micro-earthquakes. The research results show that: (1) The period of the W1123 working face mining under solid coal is relatively frequent, and the energy of microseismic events is higher than that under the mined-out area. However, the overlying rock structure under the gob is loose, broken and easy to move, showing obvious "high frequency-low energy" characteristics. (2) Extremely low values of the number and energy of microseismic events occurred in the first 3 to 5 days of the rockburst event in the working face, and the locations of the rockburst disaster in the mine were generally distributed at the edge of the low-density area of the microseismic event. The accuracy of rockburst prediction is effectively improved through multi-parameter comprehensive early warning. (3) Roof deep hole blasting and roof cutting pressure relief weaken the roof energy accumulation and the concentrated release of rock formation energy, reduce the roof activity intensity in the microseismic event gathering area, and reduce the occurrence of large-energy events, which will easily induce large shock hazards. The energy event weakens into a slow release of multiple small energy events. This research provides a reference for the safe and efficient mining of working faces in complex space environment.
Urinary Galectin-3 and CXCL-7 as biomarkers for diagnosing interstitial cystitis and correlation with clinical characteristics
Authors: Liu Junjiang , Hou Huiqing , Zhang Yunxia , Li Shoubin , Sun Fuzhen , Wang Gang , Wei Dong , Yang Tao , Gu Shouyi 
Published: February 2022

Abstract
Background: Interstitial cystitis (IC) is a chronic inflammatory disease of the bladder, while both Chemokine (C-X-C motif) ligand 7 (CXCL-7) and Galectin-3 are the molecule related inflammatory diseases.Objective: This study sought to investigate the potential feasibility of diagnosis of interstitial cystitis by measurement of the urinary level of Chemokine (C-X-C motif) ligand 7 (CXCL-7) and Galectin-3.Methods: Both interstitial cystitis patients with or without Hunner’s ulcers were recruited in the study, while healthy subjects were involved as controls. Then we collected urine samples from all the involved subjects, and the CXCL-7 level and Galectin-3 level in the supernatants of urine were measured by ELISA. Urinary creatinine concentrations (mg Cr) of involved subjects were measured to normalize the level of CXCL-7 and Galectin-3. Also the clinical data and demographic characteristics of the recruited subjects were collected. The Receiver-operating curve (ROC) analysis was performed to explore the sensitivity and specialty of both CXCL-7 and Galectin-3 to identify interstitial cystitis. And the associations between urinary levels (CXCL-7 or Galectin-3) and the symptom severity (O'Leary and Sant Symptom, OSSI index) were analyzed with linear regression.Results: The urinary levels of both CXCL-7 and Galectin-3 were significantly increased in interstitial cystitis compared to the healthy controls. The urinary CXCL-7 showed the sensitivity of 59.5% and specificity of 94.6%, at the cut-off value 21.30 pg/mg Cr to discriminate interstitial cystitis from controls, while the urinary Galectin-3 level showed the sensitivity of 62.2% and specificity of 91.9%, at the cut-off value 11.32 pg/mg Cr to discriminate interstitial cystitis from controls. Urinary Galectin-3 level was higher in interstitial cystitis patients with Hunner’s ulcers compared to patients without Hunner’s ulcers. The sensitivity and specificity were 45.5% and 93.3%, respectively for urinary CXCL-7 to discriminate from interstitial cystitis patients with Hunner’s ulcers to patients without Hunner’s ulcers, at the cut-off value of 35.97 pg/mg Cr. In addition, the urinary Galectin-3 level was positive correlated with the symptom severity.Conclusion: CXCL-7 and Galectin-3 were elevated in the interstitial cystitis patients, both of which could be supplementary biomarker for diagnosis of interstitial cystitis. Moreover, urinary CXCL-7 might serve as the biomarker to decimate interstitial cystitis with Hunner ‘s ulcers from the without Hunner’s ulcers.
Identification of ruptured intracranial aneurysms using the aneurysm-specific prediction score in patients with multiple aneurysmal subarachnoid hemorrhages
Authors: Zhang Xue-hua , Zhao Xiao-yan , Liu Lan-lan , Wen Li , Wang Guang-xian 
Published: February 2022

Abstract
Background: For patients with multiple aneurysmal subarachnoid hemorrhages (SAHs), a simple and fast imaging method that can identify ruptured intracranial aneurysms (RIAs) may have great clinical value. We sought to use the aneurysm-specific prediction (ASP) score to identify RIAs in patients with multiple IAs (MIAs) and evaluate the ASP score.Methods: Between May 2018 and May 2021, 134 patients with 290 MIAs were retrospectively analyzed. All patients had an SAH due to IA rupture. CT angiography (CTA) was used to assess the maximum diameter, shape, and location of IAs to calculate the ASP score. Then, the ASP score was applied to patients with actual RIAs. Results: The IAs with the highest ASP scores had not ruptured in 17 (12.7%) of the 134 patients with 290 MIAs. The sensitivity, specificity, false omission rate, diagnostic error rate, and diagnostic accuracy of the ASP score were higher than those of the maximum diameter, shape, and location of IAs.Conclusions: The present study suggests that the ASP score has high diagnostic accuracy in identifying RIAs in patients with MIAs and SAH, but that it needs further evaluation.
Usefulness Of A Commercial LAMP Assay For Detection Of Malaria Infection, Including Plasmodium Knowlesi Cases, In Returning Travelers In Spain
Authors: Ramírez Alexandra Martín , Lanza Marta , Hisam Shamilah , Perez-Ayala Ana , Rubio José M. 
Published: February 2022

Abstract
Objective: Main malaria diagnosis is based on microscopic examination combined with rapid diagnostic tests. Both methods have low sensitivity and specificity. Loop-mediated isothermal amplification techniques have shown a sensitivity similar to PCR but with lower times of performance.This study aimed to assess a commercial LAMP for the diagnosis of malaria (Alethia® Malaria) against the Nested-Multiplex-Malaria PCR, including the analytical sensitivity and the operational characteristics.Results: One hundred five samples out of 114 rendered valid results, obtaining 85 positive samples and 18 negative samples with an agreement of 98% compared to the reference method with a sensitivity, specificity and kappa coefficient of 98.84%, 94.74% and 0.94 respectively, with only two discrepant samples. The turnaround time was estimated in 1 hour and 30 minutes, with a cost of 32.67€ per determination. The results showed several advantages of the Alethia® Malaria, as it was easy to perform, minimal training requirement and 40 minutes run. Moreover, it includes an internal control to avoid false negatives. However, it also showed some limitations such as the need for a specific amplification and detection device, the detection of only Plasmodium spp. and a very high price.