bluuebunny/crossref_metadata_2025_split huggingface dataset:
Large‐Scale Interseismic Strain Mapping of the NE Tibetan Plateau From Sentinel‐1 Interferometry
Authors: Ou Q. , Daout S. , Weiss J. R. , Shen L. , Lazecký M. , Wright T. J. , Parsons B. E. 
Published: June 2022

AbstractThe launches of the Sentinel‐1 synthetic aperture radar satellites in 2014 and 2016 started a new era of high‐resolution velocity and strain rate mapping for the continents. However, multiple challenges exist in tying independently processed velocity data sets to a common reference frame and producing high‐resolution strain rate fields. We analyze Sentinel‐1 data acquired between 2014 and 2019 over the northeast Tibetan Plateau, and develop new methods to derive east and vertical velocities with ∼100 m resolution and ∼1 mm/yr accuracy across an area of 440,000 km2. By implementing a new method of combining horizontal gradients of filtered east and interpolated north velocities, we derive the first ∼1 km resolution strain rate field for this tectonically active region. The strain rate fields show concentrated shear strain along the Haiyuan and East Kunlun Faults, and local contractional strain on fault junctions, within the Qilianshan thrusts, and around the Longyangxia Reservoir. The Laohushan‐Jingtai creeping section of the Haiyuan Fault is highlighted in our data set by extremely rapid strain rates. Strain across unknown portions of the Haiyuan Fault system, including shear on the eastern extension of the Dabanshan Fault and contraction at the western flank of the Quwushan, highlight unmapped tectonic structures. In addition to the uplift across most of the lowlands, the vertical velocities also contain climatic, hydrological or anthropogenic‐related deformation signals. We demonstrate the enhanced view of large‐scale active tectonic processes provided by high‐resolution velocities and strain rates derived from Sentinel‐1 data and highlight associated wide‐ranging research applications.
Outperforming piezoelectric ultrasonics with high-reliability single-membrane CMUT array elements
Authors: Dew Eric B. , Kashani Ilkhechi Afshin , Maadi Mohammad , Haven Nathaniel J. M. , Zemp Roger J. 
Published: June 2022

AbstractIt has long been hypothesized that capacitive micromachined ultrasound transducers (CMUTs) could potentially outperform piezoelectric technologies. However, challenges with dielectric charging, operational hysteresis, and transmit sensitivity have stood as obstacles to these performance outcomes. In this paper, we introduce key architectural features to enable high-reliability CMUTs with enhanced performance. Typically, a CMUT element in an array is designed with an ensemble of smaller membranes oscillating together to transmit or detect ultrasound waves. However, this approach can lead to unreliable behavior and suboptimal transmit performance if these smaller membranes oscillate out of phase or collapse at different voltages. In this work, we designed CMUT array elements composed of a single long rectangular membrane, with the aim of improving the output pressure and electromechanical efficiency. We compare the performance of three different modifications of this architecture: traditional contiguous dielectric, isolated isolation post (IIP), and insulated electrode-post (EP) CMUTs. EPs were designed to improve performance while also imparting robustness to charging and minimization of hysteresis. To fabricate these devices, a wafer-bonding process was developed with near-100% bonding yield. EP CMUT elements achieved electromechanical efficiency values as high as 0.95, higher than values reported with either piezoelectric transducers or previous CMUT architectures. Moreover, all investigated CMUT architectures exhibited transmit efficiency 2–3 times greater than published CMUT or piezoelectric transducer elements in the 1.5–2.0 MHz range. The EP and IIP CMUTs demonstrated considerable charging robustness, demonstrating minimal charging over 500,000 collapse-snap-back actuation cycles while also mitigating hysteresis. Our proposed approach offers significant promise for future ultrasonic applications.
Function of PHEX mutations p.Glu145* and p.Trp749Arg in families with X-linked hypophosphatemic rickets by the negative regulation mechanism on FGF23 promoter transcription
Authors: Gan Yu-mian , Zhang Yan-ping , Ruan Dan-dan , Huang Jian-bin , Zhu Yao-bin , Lin Xin-fu , Xiao Xiao-ping , Cheng Qiong , Geng Zhen-bo , Liao Li-sheng , Tang Fa-qiang , Luo Jie-wei 
Published: June 2022

AbstractX-linked hypophosphatemic rickets (XLH) is characterized by increased circulating fibroblast growth factor 23 (FGF23) concentration caused by PHEX (NM_000444.5) mutations. Renal tubular resorption of phosphate is impaired, resulting in rickets and impaired bone mineralization. By phenotypic-genetic linkage analysis, two PHEX pathogenic mutations were found in two XLH families: c.433 G > T, p.Glu145* in exon 4 and c.2245 T > C, p.Trp749Arg in exon 22. Immunofluorescence showed that the localization of p.Glu145* and p.Trp749Arg mutant and secretory PHEX (secPHEX) changed, with decreased expression. In a HEK293T cell model co-transfected with PHEX, secPHEX, and FGF23, wild-type PHEX, secPHEX, and FGF23 proteins were distributed in the cell membrane or endoplasmic reticulum, while the mutant was located in the nuclear membrane and cytoplasm. qPCR of p.Glu145* revealed decreased PHEX and secPHEX mRNA expression in cells, with no difference in mRNA expression of p.Trp749Arg. Both mutations decreased intracellular PHEX endopeptidase activity. Western blot analysis showed decrease in mutant and secPHEX protein expression and no FGF23 protein expression in single-transfected PHEX and secPHEX cells. In cells co-transfected with FGF23, PHEX and secPHEX mutation promoted FGF23 expression. Dual-luciferase reporter gene was used to detect the effect of PHEX on FGF23 promoter. The dual-luciferase reporter gene showed that after PHEX overexpression, the activity of mutant firefly luciferase was significantly higher than that of wild type. The regulatory mechanism between PHEX and FGF23 is still unclear, but we found that PHEX is a direct transcriptional inhibitor of FGF23 and affects the expression of FGF23. This study verified the pathogenicity of the two variants and revealed the possible regulatory mechanism between PHEX and FGF23.
Direct interaction of a chaperone-bound type III secretion substrate with the export gate
Authors: Gilzer Dominic , Schreiner Madeleine , Niemann Hartmut H. 
Published: June 2022

AbstractSeveral gram-negative bacteria employ type III secretion systems (T3SS) to inject effector proteins into eukaryotic host cells directly from the bacterial cytoplasm. The export gate SctV (YscV in Yersinia) binds substrate:chaperone complexes such as YscX:YscY, which are essential for formation of a functional T3SS. Here, we present structures of the YscX:YscY complex alone and bound to nonameric YscV. YscX binds its chaperone YscY at two distinct sites, resembling the heterotrimeric complex of the T3SS needle subunit with its chaperone and co-chaperone. In the ternary complex the YscX N-terminus, which mediates YscX secretion, occupies a binding site within one YscV that is also used by flagellar chaperones, suggesting the interaction’s importance for substrate recognition. The YscX C-terminus inserts between protomers of the YscV ring where the stalk protein binds to couple YscV to the T3SS ATPase. This primary YscV–YscX interaction is essential for the formation of a secretion-competent T3SS.
Hierarchical tensile structures with ultralow mechanical dissipation
Authors: Bereyhi M. J. , Beccari A. , Groth R. , Fedorov S. A. , Arabmoheghi A. , Kippenberg T. J. , Engelsen N. J. 
Published: June 2022

AbstractStructural hierarchy is found in myriad biological systems and has improved man-made structures ranging from the Eiffel tower to optical cavities. In mechanical resonators whose rigidity is provided by static tension, structural hierarchy can reduce the dissipation of the fundamental mode to ultralow levels due to an unconventional form of soft clamping. Here, we apply hierarchical design to silicon nitride nanomechanical resonators and realize binary tree-shaped resonators with room temperature quality factors as high as 7.8 × 108 at 107 kHz frequency (1.1 × 109 at T = 6 K). The resonators’ thermal-noise-limited force sensitivities reach 740 zN/Hz1/2 at room temperature and 90 zN/Hz1/2 at 6 K, surpassing state-of-the-art cantilevers currently used for force microscopy. Moreover, we demonstrate hierarchically structured, ultralow dissipation membranes suitable for interferometric position measurements in Fabry-Pérot cavities. Hierarchical nanomechanical resonators open new avenues in force sensing, signal transduction and quantum optomechanics, where low dissipation is paramount and operation with the fundamental mode is often advantageous.
Self-assembly of highly ordered micro- and nanoparticle deposits
Authors: Zargartalebi Hossein , Hejazi S. Hossein , Sanati-Nezhad Amir 
Published: June 2022

AbstractThe evaporation of particle-laden sessile droplets is associated with capillary-driven outward flow and leaves nonuniform coffee-ring-like particle patterns due to far-from-equilibrium effects. Traditionally, the surface energies of the drop and solid phases are tuned, or external forces are applied to suppress the coffee-ring; however, achieving a uniform and repeatable particle deposition is extremely challenging. Here, we report a simple, scalable, and noninvasive technique that yields uniform and exceptionally ordered particle deposits on a microscale surface area by placing the droplet on a near neutral-wet shadow mold attached to a hydrophilic substrate. The simplicity of the method, no external forces, and no tuning materials’ physiochemical properties make the present generic approach an excellent candidate for a wide range of sensitive applications. We demonstrate the utility of this method for fabricating ordered mono- and multilayer patternable coatings, producing nanofilters with controlled pore size, and creating reproducible functionalized nanosensors.
Eliminating the need for anodic gas separation in CO2 electroreduction systems via liquid-to-liquid anodic upgrading
Authors: Xie Ke , Ozden Adnan , Miao Rui Kai , Li Yuhang , Sinton David , Sargent Edward H. 
Published: June 2022

AbstractElectrochemical reduction of CO2 to multi-carbon products (C2+), when powered using renewable electricity, offers a route to valuable chemicals and fuels. In conventional neutral-media CO2-to-C2+ devices, as much as 70% of input CO2 crosses the cell and mixes with oxygen produced at the anode. Recovering CO2 from this stream adds a significant energy penalty. Here we demonstrate that using a liquid-to-liquid anodic process enables the recovery of crossed-over CO2 via facile gas-liquid separation without additional energy input: the anode tail gas is directly fed into the cathodic input, along with fresh CO2 feedstock. We report a system exhibiting a low full-cell voltage of 1.9 V and total carbon efficiency of 48%, enabling 262 GJ/ton ethylene, a 46% reduction in energy intensity compared to state-of-art single-stage CO2-to-C2+ devices. The strategy is compatible with today’s highest-efficiency electrolyzers and CO2 catalysts that function optimally in neutral and alkaline electrolytes.
The Landé factors of electrons and holes in lead halide perovskites: universal dependence on the band gap
Authors: Kirstein E. , Yakovlev D. R. , Glazov M. M. , Zhukov E. A. , Kudlacik D. , Kalitukha I. V. , Sapega V. F. , Dimitriev G. S. , Semina M. A. , Nestoklon M. O. , Ivchenko E. L. , Kopteva N. E. , Dirin D. N. , Nazarenko O. , Kovalenko M. V. , Baumann A. , Höcker J. , Dyakonov V. , Bayer M. 
Published: June 2022

AbstractThe Landé or g-factors of charge carriers are decisive for the spin-dependent phenomena in solids and provide also information about the underlying electronic band structure. We present a comprehensive set of experimental data for values and anisotropies of the electron and hole Landé factors in hybrid organic-inorganic (MAPbI3, MAPb(Br0.5Cl0.5)3, MAPb(Br0.05Cl0.95)3, FAPbBr3, FA0.9Cs0.1PbI2.8Br0.2, MA=methylammonium and FA=formamidinium) and all-inorganic (CsPbBr3) lead halide perovskites, determined by pump-probe Kerr rotation and spin-flip Raman scattering in magnetic fields up to 10 T at cryogenic temperatures. Further, we use first-principles density functional theory (DFT) calculations in combination with tight-binding and k ⋅ p approaches to calculate microscopically the Landé factors. The results demonstrate their universal dependence on the band gap energy across the different perovskite material classes, which can be summarized in a universal semi-phenomenological expression, in good agreement with experiment.
Relative, local and global dimension in complex networks
Authors: Peach Robert , Arnaudon Alexis , Barahona Mauricio 
Published: June 2022

AbstractDimension is a fundamental property of objects and the space in which they are embedded. Yet ideal notions of dimension, as in Euclidean spaces, do not always translate to physical spaces, which can be constrained by boundaries and distorted by inhomogeneities, or to intrinsically discrete systems such as networks. To take into account locality, finiteness and discreteness, dynamical processes can be used to probe the space geometry and define its dimension. Here we show that each point in space can be assigned a relative dimension with respect to the source of a diffusive process, a concept that provides a scale-dependent definition for local and global dimension also applicable to networks. To showcase its application to physical systems, we demonstrate that the local dimension of structural protein graphs correlates with structural flexibility, and the relative dimension with respect to the active site uncovers regions involved in allosteric communication. In simple models of epidemics on networks, the relative dimension is predictive of the spreading capability of nodes, and identifies scales at which the graph structure is predictive of infectivity. We further apply our dimension measures to neuronal networks, economic trade, social networks, ocean flows, and to the comparison of random graphs.
Order-of-magnitude enhancement in photocurrent generation of Synechocystis sp. PCC 6803 by outer membrane deprivation
Authors: Kusama Shoko , Kojima Seiji , Kimura Ken , Shimakawa Ginga , Miyake Chikahiro , Tanaka Kenya , Okumura Yasuaki , Nakanishi Shuji 
Published: June 2022

AbstractBiophotovoltaics (BPV) generates electricity from reducing equivalent(s) produced by photosynthetic organisms by exploiting a phenomenon called extracellular electron transfer (EET), where reducing equivalent(s) is transferred to external electron acceptors. Although cyanobacteria have been extensively studied for BPV because of their high photosynthetic activity and ease of handling, their low EET activity poses a limitation. Here, we show an order-of-magnitude enhancement in photocurrent generation of the cyanobacteriumSynechocystissp. PCC 6803 by deprivation of the outer membrane, where electrons are suggested to stem from pathway(s) downstream of photosystem I. A marked enhancement of EET activity itself is verified by rapid reduction of exogenous electron acceptor, ferricyanide. The extracellular organic substances, including reducing equivalent(s), produced by this cyanobacterium serve as respiratory substrates for other heterotrophic bacteria. These findings demonstrate that the outer membrane is a barrier that limits EET. Therefore, depriving this membrane is an effective approach to exploit the cyanobacterial reducing equivalent(s).
The microRNA cluster C19MC confers differentiation potential into trophoblast lineages upon human pluripotent stem cells
Authors: Kobayashi Norio , Okae Hiroaki , Hiura Hitoshi , Kubota Naoto , Kobayashi Eri H. , Shibata Shun , Oike Akira , Hori Takeshi , Kikutake Chie , Hamada Hirotaka , Kaji Hirokazu , Suyama Mikita , Bortolin-Cavaillé Marie-Line , Cavaillé Jérôme , Arima Takahiro 
Published: June 2022

AbstractThe first cell fate commitment during mammalian development is the specification of the inner cell mass and trophectoderm. This irreversible cell fate commitment should be epigenetically regulated, but the precise mechanism is largely unknown in humans. Here, we show that naïve human embryonic stem (hES) cells can transdifferentiate into trophoblast stem (hTS) cells, but primed hES cells cannot. Our transcriptome and methylome analyses reveal that a primate-specific miRNA cluster on chromosome 19 (C19MC) is active in naïve hES cells but epigenetically silenced in primed ones. Moreover, genome and epigenome editing using CRISPR/Cas systems demonstrate that C19MC is essential for hTS cell maintenance and C19MC-reactivated primed hES cells can give rise to hTS cells. Thus, we reveal that C19MC activation confers differentiation potential into trophoblast lineages on hES cells. Our findings are fundamental to understanding the epigenetic regulation of human early development and pluripotency.
Dynamic self-stabilization in the electronic and nanomechanical properties of an organic polymer semiconductor
Authors: Dobryden Illia , Korolkov Vladimir V. , Lemaur Vincent , Waldrip Matthew , Un Hio-Ieng , Simatos Dimitrios , Spalek Leszek J. , Jurchescu Oana D. , Olivier Yoann , Claesson Per M. , Venkateshvaran Deepak 
Published: June 2022

AbstractThe field of organic electronics has profited from the discovery of new conjugated semiconducting polymers that have molecular backbones which exhibit resilience to conformational fluctuations, accompanied by charge carrier mobilities that routinely cross the 1 cm2/Vs benchmark. One such polymer is indacenodithiophene-co-benzothiadiazole. Previously understood to be lacking in microstructural order, we show here direct evidence of nanosized domains of high order in its thin films. We also demonstrate that its device-based high-performance electrical and thermoelectric properties are not intrinsic but undergo rapid stabilization following a burst of ambient air exposure. The polymer’s nanomechanical properties equilibrate on longer timescales owing to an orthogonal mechanism; the gradual sweating-out of residual low molecular weight solvent molecules from its surface. We snapshot the quasistatic temporal evolution of the electrical, thermoelectric and nanomechanical properties of this prototypical organic semiconductor and investigate the subtleties which play on competing timescales. Our study documents the untold and often overlooked story of a polymer device’s dynamic evolution toward stability.
Giant energy-storage density with ultrahigh efficiency in lead-free relaxors via high-entropy design
Authors: Chen Liang , Deng Shiqing , Liu Hui , Wu Jie , Qi He , Chen Jun 
Published: June 2022

AbstractNext-generation advanced high/pulsed power capacitors rely heavily on dielectric ceramics with high energy storage performance. However, thus far, the huge challenge of realizing ultrahigh recoverable energy storage density (Wrec) accompanied by ultrahigh efficiency (η) still existed and has become a key bottleneck restricting the development of dielectric materials in cutting-edge energy storage applications. Here, we propose a high-entropy strategy to design “local polymorphic distortion” including rhombohedral-orthorhombic-tetragonal-cubic multiphase nanoclusters and random oxygen octahedral tilt, resulting in ultrasmall polar nanoregions, an enhanced breakdown electric field, and delayed polarization saturation. A giant Wrec ~10.06 J cm−3 is realized in lead-free relaxor ferroelectrics, especially with an ultrahigh η ~90.8%, showing breakthrough progress in the comprehensive energy storage performance for lead-free bulk ceramics. This work opens up an effective avenue to design dielectric materials with ultrahigh comprehensive energy storage performance to meet the demanding requirements of advanced energy storage applications.
Climate change reshuffles northern species within their niches
Authors: Antão Laura H. , Weigel Benjamin , Strona Giovanni , Hällfors Maria , Kaarlejärvi Elina , Dallas Tad , Opedal Øystein H. , Heliölä Janne , Henttonen Heikki , Huitu Otso , Korpimäki Erkki , Kuussaari Mikko , Lehikoinen Aleksi , Leinonen Reima , Lindén Andreas , Merilä Päivi , Pietiäinen Hannu , Pöyry Juha , Salemaa Maija , Tonteri Tiina , Vuorio Kristiina , Ovaskainen Otso , Saastamoinen Marjo , Vanhatalo Jarno , Roslin Tomas , Laine Anna-Liisa 
Published: June 2022

AbstractClimate change is a pervasive threat to biodiversity. While range shifts are a known consequence of climate warming contributing to regional community change, less is known about how species’ positions shift within their climatic niches. Furthermore, whether the relative importance of different climatic variables prompting such shifts varies with changing climate remains unclear. Here we analysed four decades of data for 1,478 species of birds, mammals, butterflies, moths, plants and phytoplankton along a 1,200 km high latitudinal gradient. The relative importance of climatic drivers varied non-uniformly with progressing climate change. While species turnover among decades was limited, the relative position of species within their climatic niche shifted substantially. A greater proportion of species responded to climatic change at higher latitudes, where changes were stronger. These diverging climate imprints restructure a full biome, making it difficult to generalize biodiversity responses and raising concerns about ecosystem integrity in the face of accelerating climate change.
A dimeric proteomimetic prevents SARS-CoV-2 infection by dimerizing the spike protein
Authors: Khatri Bhavesh , Pramanick Ishika , Malladi Sameer Kumar , Rajmani Raju S. , Kumar Sahil , Ghosh Pritha , Sengupta Nayanika , Rahisuddin R. , Kumar Narender , Kumaran S. , Ringe Rajesh P. , Varadarajan Raghavan , Dutta Somnath , Chatterjee Jayanta 
Published: June 2022

AbstractProtein tertiary structure mimetics are valuable tools to target large protein–protein interaction interfaces. Here, we demonstrate a strategy for designing dimeric helix-hairpin motifs from a previously reported three-helix-bundle miniprotein that targets the receptor-binding domain (RBD) of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). Through truncation of the third helix and optimization of the interhelical loop residues of the miniprotein, we developed a thermostable dimeric helix-hairpin. The dimeric four-helix bundle competes with the human angiotensin-converting enzyme 2 (ACE2) in binding to RBD with 2:2 stoichiometry. Cryogenic-electron microscopy revealed the formation of dimeric spike ectodomain trimer by the four-helix bundle, where all the three RBDs from either spike protein are attached head-to-head in an open conformation, revealing a novel mechanism for virus neutralization. The proteomimetic protects hamsters from high dose viral challenge with replicative SARS-CoV-2 viruses, demonstrating the promise of this class of peptides that inhibit protein–protein interaction through target dimerization.