bluuebunny/crossref_metadata_2025_split huggingface dataset:
A non-invasive risk score including skin autofluorescence predicts diabetes risk in the general population
Authors: Wolffenbuttel Bruce H.R. , Boersma Henderikus E. , Klauw Melanie M. , Smit Andries J. 
Published: June 2022

Abstract
Increased skin autofluorescence (SAF) predicts the development of diabetes-related complications and cardiovascular disease. We assessed the performance of a simple model which includes SAF to identify individuals at high risk for undiagnosed and incident type 2 diabetes, in 58,377 participants in the Lifelines Cohort Study without known diabetes. Newly-diagnosed diabetes was defined as fasting blood glucose ≥7.0 mmol/l and/or HbA1c ≥6.5% (≥48 mmol/mol) or self-reported diabetes at follow-up. We constructed predictive models based on age, body mass index (BMI), SAF, and parental history of diabetes, and compared to results with the concise FINDRISC model.At 2nd visit to Lifelines, 1113 (1.9%) participants were identified with undiagnosed diabetes and 1033 (1.8%) participants developed diabetes during follow-up. A model comprising age, BMI and SAF yielded an AUC of 0.783 and was non-inferior to the concise FINDRISC model, which had an AUC of 0.797 to predict new diabetes. At a score of 5.8, sensitivity was 78% and specificity of 66%. Model 2 which also incorporated parental diabetes history, had an AUC of 0.792, and a sensitivity of 74% and specificity of 70% at a score of 6.5. Net reclassification index (NRI) did not improve significantly (NRI 1.43% (-0.50-3.37 p=0.15). The combination of an easy to perform SAF measurement with age and BMI is a good alternative screening tool suitable for medical and non-medical settings. Parental history of diabetes did not significantly improve model performance in this homogeneous cohort.
Extract From Leaf of Epipremnum Aureum on the Concert of Dye Sensitized Solar Cell
Authors: Kavitha A. , Karthick S. , Pavithra S. , Devi N. S.M. P. Latha , Shanmugan S. 
Published: June 2022

Abstract
Sensitizers for Dye-Sensitized Solar Cell (DSSC) significantly impact the electron-hole pair for conduction to produce photocurrent. In this study, sensitizers from the leaf extract of Epipremnum aureum have been subjected to sensitize the solar cell with ethanol as solvent, and the photoconversion efficiency has been evaluated. Two dyes have been used, viz. The first dye is the natural dye alone, and the second is the cocktail of natural dye (dye from Epipremnum aureum) and synthetic eosin dye. The fabricated solar cells are subjected to UV-Vis, FTIR, XRD, SEM, and J-V characteristics to study the solar cell's performance and the effectiveness of the dye. The photoconversion efficiency of the solar cell with the cocktail of natural and synthetic dye is higher than the cell with natural and synthetic dye alone. It has been found that the cell with a cocktail of dyes has given an efficiency of 0.78%, which is higher than the solar with eosin or natural dye alone.
Covid-19 Pandemic: A Qualitative Study on Adolescents’ Stress, Anxiety, and Family Communication
Authors: Çetin Zeynep 
Published: June 2022

Abstract
Eight high-school students participated in this qualitative study to investigate the adolescents’ stress, anxiety, and family communication during the COVID-19 pandemic. To collect data, a list of questions was prepared by the researchers based on experts’ opinions, and open-ended interviews were carried out with the participants. Consequently, four models were created and thoroughly investigated. According to the results, the COVID-19 pandemic led to higher levels of stress and anxiety and both positive and negative effects in the adolescents’ communication with their families. It seems that the period of social isolation adversely affected the adolescents’ interaction with their peers.
A Chinese kindred report of Charcot-Marie-Tooth Disease Type 4C with the novel compound heterozygous variants in the SH3TC2 gene and a systematic review
Authors: Zhang Jingfei , Wang Hong , Wang Juan , Zhang Jing , Yang Wenqu , Pang Xiaomin , Zhang Wei , Guo Junhong , Chang Xueli 
Published: June 2022

Abstract
Charcot-Marie-Tooth disease type 4C (CMT4C) is an autosomal recessive neuropathy caused by SH3TC2 mutations, characterized clinically by early-onset severe scoliosis, foot deformities, predominantly distal weakness, muscle atrophy, sensory loss and cranial nerve deficits. Most patients carry missense mutations and nonsense mutations in chromosome 5q23-33 of SH3TC2 gene associated with Schwann cells. Through linkage, haplotype and sequencing analysis in a kindred from northern China with three members affected by autosomal recessive peripheral neuropathy, we identified the compound heterozygous mutations Q244X and L1048P in SH3TC2. Onset age ranged from 6 to 43 years and all three of them had hearing loss as their first symptom. Neuropathy was moderate-to-severe. Scoliosis, foot deformities, predominantly distal weakness, muscle atrophy, sensory loss and cranial nerve deficits were present in all of them. Our study of this family expands the spectrum of mutations causing CMT4C with the discovery of a novel nonsense mutation in the SH3TC2 gene. Further more, due to the weak correlation between the nature and the position of the pathogenic variant, disease duration, and the stage of disability of CMT4C, we conducted a systematic review and evaluation of the previous literatures to further analyze the clinical findings of CMT4C.
Design of a Virtual Mouse Using Gesture Recognition and Machine Learning
Authors: Joshi Hritik , waybhase Nitin , Litoriya Ratnesh , Mangal Dharmendra 
Published: June 2022

Abstract
The emergence of the pandemic has resulted in a technological paradigm shift. The development and use of a virtual mouse in place of a real mouse is one such field of research. This is a step forward in the field of HMI (Human-Machine Interaction). Even if wireless or Bluetooth mouse technology has yet to be established, it is not device-free. A Bluetooth mouse necessitates the usage of a battery and a connected dongle. The presence of additional electronics in a mouse makes it more difficult to use. This system takes frames using a webcam or built-in camera, analyses them to make them trackable, and then detects and performs mouse functions based on the gestures performed by users. As a result, the suggested mouse system eliminates the need for a device.
Consistency and prognostic value of preoperative staging and postoperative pathological staging using 18 F-FDG PET/MRI in patients with non-small cell lung cancer
Authors: Kajiyama Akiko , Ito Kimiteru , Watanabe Hirokazu , Mizumura Sunao , Watanabe Shun-ichi , Yatabe Yasushi , Gomi Tatsuya , Kusumoto Masahiko 
Published: June 2022

Abstract
Background
In recent years, PET/MRI has been used clinically as a method to diagnose non-small cell lung cancer (NSCLC). However, no study has shown the usefulness of PET/MRI for lung tumor staging or its consistency with pathological staging compared to CT. This study aimed to evaluate the concordance of staging and prognostic ability of NSCLC patients using thin-section CT and 18F-FDG PET/MRI.
Methods
This retrospective study was performed on consecutive NSCLC patients who underwent both diagnostic CT and 18F-FDG PET/MRI before surgery between November 2015 and May 2019. The cTNM staging yielded from PET/MRI was compared with CT and pathological staging, and concordance was investigated. To assess the prognostic value of disease-free survival (DFS) and overall survival (OS), we dichotomized the typical prognostic factors and TNM classification staging (Stage I vs. Stage II or higher). Kaplan-Meier curves derived by the log-rank test were generated, and univariate and multivariate analyses were performed to identify the factors associated with DFS and OS.
Results
A total of 82 subjects were included; PET/MRI staging was more consistent (59 of 82) with pathological staging than with CT staging. There were a total of 21 cases of CT and 11 cases of PET/MRI that were judged as cStage I but were actually pStage II or pStage III. CT tended to judge pN0 as cN1 or cN2 compared to PET/MRI. There was a significant difference between NSCLC patients with Stage I and Stage II or higher by PET/MRI staging as well as prognosis prediction of DFS by pathological staging (P < 0.001). In univariate analysis, PET/MRI, CT, and pathological staging (Stage I or lower vs. Stage II or higher) all showed significant differences. In multivariate analysis, pathological staging was the only independent factor for recurrence (P = 0.009), and preoperative PET/MRI staging was a predictor of patient survival (P = 0.013).
Conclusions
In NSCLC, pathologic staging was better at predicting recurrence, and preoperative PET/MRI staging was better at predicting the patients’ survival. Preoperative staging by PET/MRI was superior to CT in diagnosing hilar and mediastinal lymph node metastases, which contributed to the high concordance with pathologic staging.
An improvement of heterogeneous catalyst Fe3O4@MnO2 nanocatalyst on bio-diesel production from castor oil
Authors: SARAVANAN SAKTHI , Sivanandi Periyasamy 
Published: June 2022

Abstract
The exhaustion of natural resources has occasioned the profit margin of oil products to increase dramatically, prompting the use of sustainable fuels like as biofuel. In the proposed investigation, biofuel was developed from castor oil utilizing Fe3O4@MnO2 NCs as a heterogeneous catalyst for the transesterification process. X-Ray Diffraction evaluation acknowledged the nanocatalyst's single phase. Scanning Electron Microscopy revealed the spherical morphology of the consolidated nanocatalyst. The magnetic characteristics were examined using a vibrating sample magnetometer. Atomic Force Microscopy demonstrated that the catalyst exhibited has a greater surface area and unevenness. The yield was achieved was 95 percent (w/w) in 50 minutes at 600 C with 15 wt percent catalyst loading and a 12:1 methanol/oil ratio, as acknowledged by a gas chromatograph with mass spectrometer. The findings indicate that Fe3O4@MnO2 NCs are an encouraging catalyst for bio-fuels production via heterogeneous catalyzed transesterification under relatively mild response circumstances.
Cytoplasmic APE1 promotes lung cancer aggressiveness and cisplatin resistance via the COX-2/Akt/β-catenin pathway
Authors: Lian Xiaojuan , Yue Hongxu , Xiang Debing , Zhang Zhimin 
Published: June 2022

Abstract
Background
Cisplatin is commonly used in lung cancer therapy, but cisplatin resistance in lung cancer cells remains an unsolved problem. Here, we report that cytoplasmic APE1 contributes to cisplatin resistance, cell proliferation and migration in lung cancer cells.
Methods
Immunofluorescence, western blot analysis, lentivirus transfection and scratch assays, and transwell migration and invasion assays were carried out on the cell lines A549 and Calu-1. A total of 124 samples of lung cancer tissues were evaluated to determine the clinical effects of cytoplasmic APE1 and COX-2.
Results
We found that cytoplasmic APE1 expression was lower in cisplatin sensitive cells than in cisplatin-resistant cells, and the upregulation of cytoplasmic APE1 significantly reduced cisplatin sensitivity in lung cancer cells. Gain-of-function studies demonstrated that cytoplasmic APE1 promoted lung cancer cell proliferation, migration and invasion in vitro and tumor growth in vivo, which were inhibited after cisplatin treatment. In patient samples, cytoplasmic APE1 in lung cancer tissues was an independent indicator of overall survive (OS) for lung cancer patients (P < 0.001). Mechanistic studies revealed that cytoplasmic APE1 promotes lung cancer malignancy by activating the COX-2/Akt/β-catenin pathway, and that APE1-C65 site mutations can increase cytoplasmic APE1 expression and resistance to cisplatin in lung cancer cells.
Conclusion
We suggest that modulating cytoplasmic APE1 in lung cancer is a promising novel strategy for overcoming cisplatin resistance.
Analysis of metabolites of fungal balls in the paranasal sinuses
Authors: ZHANG Xiaoqing , ZHANG Na , HUANG Qian , CUI Shunjiu , LIU Lingyan , ZHOU Bing 
Published: June 2022

Abstract
Fungal ball sinusitis is characterized by complex fungus infections with non-invasive inflammation. But no research reported fungal ball composition and metabolic-related product types currently. 12 patients with chronic rhinosinusitis who underwent surgery and 9 healthy control were enrolled in this study. Samples from both groups were analyzed for high-throughput metabolites by UPLC-MS. OsiriX software was applied to perform imaging measurements on sinus CT. 2138 and 394 metabolites were screened from cationic and anionic modes. There was a significant difference in the abundance of glycerophospholipid metabolism and sphingolipid metabolism between the two groups, with the experimental group showing an increased trend related to the sphingolipid metabolic pathway, including sphingosine 1-phosphate (S1P) and related products, diacylglycerol (DG), sphingomyelin (SM), suggesting that its metabolites are associated with mucosal and bony inflammation. Imaging measurements showed a median sinus CT value (median (P25, P75) of 351(261.4, 385.8) HU and a median sinus wall thickness (median (P25, P75) of 2.31(1.695, 3.718) mm, which correlated with the levels of glycerophospholipid metabolites and sphingolipid metabolites (P < 0.03). Dysfunctional glycerophospholipid and sphingolipid metabolism is present in the lesion of fungal ball sinusitis. Glycerophospholipid and sphingolipid metabolism plays a significant role in the progression of mucosal and osteitis produced by fungal ball sinusitis.
Molecular characterization, expression and localization analysis of a pedal like peptide in cuttlefish Sepiella japonica
Authors: Li Gong , Qiu Jiayin , Cao Huimin , Li Shuang , Zheng Libing , Chi Changfeng 
Published: June 2022

Abstract
BackgroundNeuropeptide pedal peptide (PP) and orcokinin (OK) are related active peptides that have been widely discovered in invertebrates. They have complex structures, and play key roles in various physiological process. Till now, there were no any reports in cephalopods with highly differentiated multi-lobular brain.MethodsThe partial cDNA sequence of neuropeptide PP in Sepiella japonica (termed as Sj-PP) was identified from a transcriptome, the full-length cDNA was obtained by rapid amplification cDNA ends (RACE) method. The bioinformatic analysis was performed through associated softwares. The tissue distribution profile and spatio-temporal expression were detected out by semi-quantitative RT-PCR and quantative Real-time PCR (qRT-PCR) assay, respectively. The tissue location was carried out with in situ hybridization (ISH).ResultsThe putative precursor of Sj-PP encodes 22 mature peptides, of which, only tridecapeptides could be post-translationally amidated at C-terminus. Each tridecapeptide owns the most conserved and frequent N-terminus DSI. The sequence analysis showed PPs shared low identity, and Sj-PP might be a PP3 member. The quantitative data showed Sj-PP widely distributed in various tissues, and the content in brain, optic lobe, liver and nidamental gland continuously increased till gonad maturation during the whole growth and development stages. In situ hybridization (ISH) detected Sj-PP positive signals in almost all regions of optic lobe except plexiform zone, all functional lobes in brain, epithelial cells and outer membrane layer of accessary nidamental gland, which hinted it is likely to be involved in the regulation of reproduction, such as vitellogenin synthesis, restoration, and ova encapsulation.ConclusionsThe results declared Sj-PP was involved in the neuroendocrine regulation in cephalapod, and provided a basic theoretical data for further studying regulatory role in the reproduction.
STIM1-mediated SOCE Promotes Autophagy through AKT/mTOR Pathway in Hippocampal Neurons after Ischemic Stroke
Authors: Zhang Hongchen , Xie Wenyu , Feng Yuan , Wei Jialiang , Yang Changbin , Luo Peng , Yang Yuefan , Zhao Peng , Jiang Xiaofan , Liang Wenbin , Dai Shuhui , Li Xia 
Published: June 2022

Abstract
Disturbance of calcium homeostasis and autophagy are two major pathogenesis in cerebral ischemic-induced neuronal injury. However, the interconnection of these two pathways and downstream target during injury remains unclear. Stromal interaction molecule 1 (STIM1) acts as a calcium sensor and activates Orai1 calcium channels, triggering store-operated Ca2+ entry (SOCE). Recently, STIM1 has also been shown to promote autophagy in multiple cell lines. Previously we have discovered that, under hypoxia condition, the AKT/mTOR signaling pathway plays a protective role in oxygen and glucose deprivation (OGD)-induced neuronal damage by reducing autophagic activity. In the present study, we provide preclinical studies supporting that inhibition of autophagy through the STIM1/SOCE/mTOR pathway can achieve neuronal protection during ischemic stroke. Using a mouse model of transient middle cerebral artery occlusion (tMCAO) and an in vitro culture of primary neurons, we found that STIM1 depletion reduced autophagic activity by inhibiting STIM1-induced SOCE, thereby attenuating neuron damage under hypoxia conditions. The inhibitory effect could be reversed by AZD5363, an AKT/mTOR pathway inhibitor, demonstrating that AKT/mTOR regulates STIM1/SOCE-induced autophagy. This work supports the potential STIM1 inhibition to protect neuronal damage during ischemic stroke.
Early contact with anesthesiologists at anesthesiology consulting clinic and preoperative anxiety remission in the parturients scheduled for cesarean section: A case control study
Authors: Zheng Lanlan , Li Jun , Xue Hang , Zhang Xinping , Ni Fanshu , Wu Qijun , Zhang Hehua , Zhao Ping 
Published: June 2022

Abstract
Background: Surgery and anesthesia can be traumatic for parturients scheduled for cesarean section, leading to anxiety, and anxiety may accumulate during preoperative period. We conducted this study to determine if early contact with anesthesiologists could contribute to relieving preoperative anxiety of parturients scheduled for cesarean section.Method: This is a single center case control study. From June 1, 2021, to August 30, 2021, parturients scheduled for cesarean section surgery with intraspinal anesthesia, ASAⅠ~Ⅲ, without preoperative psychiatric disorder. The State Anxiety Inventory was used to estimate the participants’ preoperative anxiety symptoms one day preoperative. Participants who sought to communicate with anesthesiologists at the anesthesiology consulting clinic were categorized as having early contact with anesthesiologists. Multiple logistic regression analysis was used to explore the association between early contact with anesthetists and preoperative anxiety symptoms. The odds ratio (95% confidence interval [CI]) of early contact with anesthesiologists in relation to the preoperative anxiety of the parturients (measured by State Anxiety Inventory). Multiple linear regression and stratified analysis using the Edinburgh Postnatal Depression Scale (EPDS) score were used for sensitivity analysis. Results: A total of 226 participants completed the study; 83 (36.7%) experienced moderate-to-severe preoperative anxiety, with 45.37 ± 5.31 State Anxiety Inventory points. Early contact with anesthesiologists was associated with a lower risk of moderate-to-severe preoperative anxiety (odds ratio = 0.23; 95% confidence interval: 0.10, 0.52) and lower anxiety score (β = -4.18, 95% CI: -6.32, -2.05). The results of the stratified analysis by depression level (measured by Edinburgh Postnatal Depression Scale) showed that this association was also statistically significant among parturients with lower (depression score <10) and higher (depression score ≥10) depression levels. Conclusion: Early contact with anesthesiologists is associated with lower risk of preoperative anxiety for parturients scheduled for cesarean section. It suggested that anesthesia consulting clinic is clinical significant and could routinely serve parturients.Registration: This study was registered in chictr.org.cn, on 28/05/2021; identifier ChiCTR2100046798)
A Pan-Cancer Analysis of the Oncogenic Role of Insulin-Like Growth Factor2 mRNA -Binding Protein 2 (IGF2BP2) in Human Tumors
Authors: Zeng Yu , Liu Yu , Zhuang Gaojian , Hao Weijing , Li Lijuan , Ma Weike , Gu Pengfei , Xie Wenjun , Zheng Xiangqian , Ruan Xianhui , Gao Ming 
Published: June 2022

Abstract
N6-methyladenosine (m6A) is one of the most common post-transcriptional modifications of eukaryotic mRNA, accounting for approximately 80% of RNA methylation modifications. Increasing studies have demonstrated that m6A modification has an important role in the development of a variety of tumors. Insulin-like growth factor 2 (IGF2BP2), as an important methylation recognition proteins in the process of m6A modification, is similarly involved in the progression of human cancers. Therefore, based on The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets, we first explored the potential oncogenic role of IGF2BP2 in thirty-three tumors and found that IGF2BP2 was highly expressed in most cancers, and it is also strongly associated with poor prognosis in some tumor patients. We performed the first pan-cancer analysis of the m6A methylation recognition protein IGF2BP2with the main goal of gaining a more comprehensive understanding of the oncogenic role of IGF2BP2 in different tumors.
Rare Benign Liver Tumors That Require Differentiation from Hepatocellular Carcinoma: Focus on Diagnosis and Treatment
Authors: Luo Laihui , Wang Tao , Cheng Mengting , Ge Xian , Song Shengjiang , Zhu Guoqing , Xiao Yongqiang , Deng Wei , Xie Jin , Shan Ren Feng 
Published: June 2022

Abstract
Background/Aim: Recently, an increase in the number of asymptomatic rare benign liver tumors (BLTs) has been reported during health check-ups. It is difficult to determine the nature of partial rare BLTs and not easy to distinguish from malignant liver tumors. This study aimed to analysis clinical features, diagnosis, and treatment of rare BLTs to reduce misdiagnosis and provide reference for clinical practice.Methods: From January 2012 to January 2021, we treated 112 rare BLTs by hepatectomy, including 54 focal nodular hyperplasia, 14 hepatocellular adenoma, 28 hepatic angiomyolipoma, 3 hepatic granuloma, 2 inflammatory pseudotumor of the liver, 2 nodular regenerative hyperplasia, 2 hepatic lipoma, 1 solitary fibrous tumor of the liver, 1 hepatic schwannoma and 1 hepatic myelolipoma.Results: The majority of patients were middle-aged female and asymptomatic. Single tumors were dominant. The diagnostic accuracies of computed tomography (CT) and magnetic resonance imaging (MRI) were 32.5% and 44.2%, respectively. The majority of tumors were likely to be misdiagnosed as hepatocellular carcinoma (HCC) or difficult to distinguish from HCC. All patients underwent surgical treatment. Postoperative pathological and immunohistochemical examination can confirm the diagnosis. No patients without tumor recurrence or metastasis during follow up period.Conclusion: Altogether, the clinical symptoms of rare BLTs lack specificity, and their preoperative diagnosis largely depends on imaging examination, with a low diagnostic accuracy rate and high chances of misdiagnosis as HCC. Diagnosis is confirmed by pathological and immunohistochemical examination. Surgical resection for rare BLT is safe and effective, regular postoperative follow-up is necessary.