bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic Therapeutic Effects of Intracerebral Transplantation of Human Modified Bone Marrow-Derived Stromal Cells (SB623) and Voluntary Exercise with Running Wheel in a Rat Model of Ischemic Stroke
Authors: Yabuno Satoru , Yasuhara Takao , Nagase Takayuki , Kawauchi Satoshi , Sugahara Chiaki , Okazaki Yosuke , Hosomoto Kakeru , Sasada Susumu , Sasaki Tatsuya , Tajiri Naoki , Borlongan Cesar V. , Date Isao 
Published: July 2022

Abstract
Background
Mesenchymal stromal cell (MSC) transplantation therapy is a promising therapy for stroke patients. In parallel, rehabilitation with physical exercise could ameliorate stroke-induced neurological impairment. In this study, we aimed to clarify whether combination therapy of intracerebral transplantation of human modified bone marrow-derived MSCs, SB623 cells, and voluntary exercise with running wheel (RW) could exert synergistic therapeutic effects on a rat model of ischemic stroke.
Methods
Wistar rats received right transient middle cerebral artery occlusion (MCAO). Voluntary exercise (Ex) groups were trained in a cage with RW from day 7 before MCAO. SB623 cells (4.0×105 cells/µl) were stereotactically injected into the right striatum at day 1 after MCAO. Behavioral tests were performed at day 1, 7, and 14 after MCAO using the modified Neurological Severity Score (mNSS) and cylinder test. Rats were euthanized at day 15 after MCAO for mRNA level evaluation of ischemic infarct volumes, endogenous neurogenesis, angiogenesis, and expression of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF). The rats were randomly assigned to one of the four groups: vehicle, Ex, SB623, and SB623 + Ex groups.
Results
SB623 + Ex group achieved significant neurological recovery in mNSS compared to the vehicle group (p < 0.05). The cerebral infarct volumes of SB623 + Ex group were significantly decreased compared to those in all other groups (p < 0.05). The number of BrdU/Doublecortin (Dcx) double-positive cells in the subventricular zone (SVZ) and the dentate gyrus (DG), the laminin-positive area in the ischemic penumbra, and the mRNA level of BDNF and VEGF in SB623 + Ex group were much significantly increased compared to those in all other groups (p < 0.05).
Conclusions
This study suggests that combination therapy of intracerebral transplantation SB623 cells and voluntary exercise with RW achieves robust neurological recovery and synergistically promotes endogenous neurogenesis and angiogenesis after cerebral ischemia, possibly through a mechanism involving the up-regulation of BDNF and VEGF.
Intrathecal injection of human umbilical cord mesenchymal stem cells genetically engineered to overexpress Heme oxygenase 1 promotes symptoms recovery in cyclophosphamide-induced cystitis rats by alleviating neuroinflammation
Authors: Gao Qiongqiong , Gao Zhentao , Huang Yong , Zhang Chi , Su Minzhi , Li Cuiping , Zhan Hailun , Liu Bolong , Zhou Xiangfu 
Published: July 2022

Abstract
Background
Interstitial cystitis/bladder pain syndrome (IC/BPS) seriously affect the patient's quality of life, yet current therapies for IC/BPS only provide partial relief. Neuroinflammation in the spinal dorsal horn (SDH) plays a pivotal role in the pathogenesis. Injection of human umbilical mesenchymal stem cells (hUMSCs) represent an effective strategy to reduce inflammation, and heme oxygenase-1 (HO-1) has anti-nociceptive effect in neuro inflammatory pain. This study aimed to tested the therapeutic effects of hUMSCs overexpressing HO-1 on cyclophosphamide-induced cystitis rat model.
Methods
Cystitis rats were transplanted with altered cells and then assessed for 3 weeks on a battery of behavioral tests that measured suprapubic mechanical allodynia, micturition frequency, depressive-like behaviors and short-term memory function. Additionally, immunofluorescence staining, western blot and Elisa kit measured the anti-inflammation effects.
Results
HUMSCs were capable of being transduced to overexpress HO-1 in vitro and in vivo. Treatment with hUMSCs overexpressing HO-1 was more effective than hUMSCs alone in alleviating suprapubic mechanical allodynia and frequent micturition in cystitis rats. Furthermore, rats treated with hUMSCs overexpressing HO-1 relieved the comorbid depressive-like behaviors and memory deficits. In the SDH region, hUMSCs overexpressing HO-1 inhibited the activation of glial, decreased the levels of pro-inflammatory cytokines by downregulating the TLR4/p65/NLRP3 pathway. And surprisingly, it markedly increased anti-inflammatory cytokine IL-10, while reduced MDA content and protected GSH concentrations in local environment.
Conclusions
Overall, our result suggest that intrathecal injection of hUMSCs overexpressing HO-1 can significantly promote functional outcomes in cystitis rats by reducing neuroinflammation, at least, partly through downregulating TLR4/p65/NLRP3 signaling pathway in the SDH region. This cell therapy providing a possible new strategy for treating IC/BPS.
Spatial pattern and density of woody plants in an agroforestry parkland in a West African savanna
Authors: Lawer Eric Adjei 
Published: July 2022

Abstract
Agroforestry parklands are an age-old traditional land use practice that integrates crop cultivation under scattered woody plants. This practice is widespread in West African savannas providing many essential ecological and socio-economic benefits to people such as food, fuelwood and medicine. Currently, parklands are decreasing due to changes in agriculture and land use practices, often associated with human population growth. Understanding spatial patterns as well as identifying reliable methods of sampling to estimate density of woody plants is necessary for sustainable management of parklands. In this study, five relatively easy-to-use plotless sampling methods were applied to estimate density of woody plants using field and simulated datasets with known spatial patterns from field assessments. Results of spatial indices tests indicated that woody plants in parklands exhibited two spatial patterns: i.e., aggregate and random, the latter being the dominant pattern observed in field datasets. Based on relative measure statistics (i.e., RRMSE and RBIAS), the ordered distance (OD), point-centered quarter (PCQ) and closest individual (CI) methods performed well when woody plants were located in a random pattern while the variable area transect (VAT) method was better at estimating density under patterns of spatial aggregation. Overall, OD and VAT methods are recommended for density estimation in parklands because they are relatively more accurate, less biased, practical and computations are easy to undertake.
The role of c-Jun for beating cardiomycyte formation in prepared embryonic body
Authors: Su Lide , Zhang Guofu , Lili Jiang , Chi Chao , Bai Bing , Kang Kai 
Published: July 2022

Abstract
Background
Morbidity and mortality associated with cardiovascular diseases, such as myocardial infarction, stem from the inability of terminally differentiated cardiomyocytes to regenerate, and thus repair the damaged myocardial tissue structure. The molecular biological mechanisms behind the lack of regenerative capacity for those cardiomyocytes remains to be fully elucidated. Recent studies have shown that c-Jun serves as a cell cycle regulator for somatic cell fates, playing a key role in multiple molecular pathways, including the inhibition of cellular reprogramming, promoting angiogenesis, and aggravation of cardiac hypertrophy, but its role in cardiac development is largely unknown. This study aims to delineate the role of c-Jun in promoting early-stage cardiac differentiation.
Methods
The c-Jun gene in mouse embryonic stem cells (mESCs) was knocked out with CRISPR-Cas9, and the hanging drop method used to prepare the resulting embryoid bodies. Cardiac differentiation was evaluated up to 9 days after c-Jun knockout (ko) via immunofluorescence, flow cytometric, and qPCR analyses.
Results
Compared to the wild-type control group, obvious beating was observed among the c-Jun-ko mESCs after 6 days, which was also associated with significant increases in myocardial marker expression. Additionally, markers associated with mesoderm and endoderm cell layer development, essential for further differentiation of ESCs into cardiomyocytes, were also up-regulated in the c-Jun-ko cell group.
Conclusions
Knocking out c-Jun directs ESCs towards a meso-endodermal cell lineage fate, in turn leading to generation of beating myocardial cells. Thus, c-Jun plays an important role in regulating early cardiac cell development.
Will Middle-Aged and Elderly be Shielded by Professional Help? Evaluation of China’s Long-Term Care Insurance: Evidence from a National Survey
Authors: Fu Jiahong 
Published: July 2022

Abstract
Background: China launched national long-term care insurance (LTCI) policy experiment in 2016, responding to the challenges in population aging. This paper aimed to identify the policy impact of China’s pilot LTCI on formal care availability, proxied by people’s belief in profession help with future difficulties in activities of daily living. Methods: This study employed a quasi-experiment design, adopting a non-linear Difference-in-Differences method to estimate the LTCI policy effect. Our data were drawn from four waves of China Health and Retirement Longitudinal Survey (CHALRS). We included parallel trends test and placebo examination as robustness tests. Results: Our findings indicated that the implementation of LTCI significantly increased people’s confidence in receiving future professional help (β=1.195, p<0.05). The treatment effect under different measurement methods ranged between 0.014 and 0.022. In addition, our heterogeneity analysis reflected that the treatment effect varied among different subgroups. Conclusions: Formal long-term care (LTC) should be emphasized more by policymakers and academics. The traditional informal care system, which depends on care services mainly from family members, is no longer sustainable due to changes in the Chinese family structure. Formal LTC should be prioritized with informal care as a supplement, and more resources input in the LTCI system are needed.
Analysis of Relevant Factors Influencing Size of Breast Abscess Cavity during Lactation: A Cross-sectional Study
Authors: Yajun Gao , Yan Zou , Yi Zhang , si Chen , yan Li , Songtao Ding 
Published: July 2022

Abstract
Objective: The aim of this study was to investigate risk factors for the severity of breast abscess during lactation .Methods: A cross-sectional study was conducted using data from the Questionnaire survey of breast abscess patients. According to whether the maximum abscess diameter > 5 cm, the patients were divided into 2 groups for univariate and multivariate regression analysis. Results: 1,805 valid questionnaires were included . Univariate and Binary logistic regression analysis demonstrated that low education (OR = 1.5, 95% CI: 1.1-2.0, P = 0.005), non-exclusive breastfeeding (OR = 0.7, 95% CI: 0.6-0.9, P = 0.004), fever > 37.5℃ (OR = 0.7, 95% CI: 0.6-0.9, P = 0.003), flat or inverted nipples (OR = 0.7, 95% CI: 0.6-0.9, P = 0.005), antibiotic used (OR = 0.7, 95% CI: 0.6-0.9, P = 0.006), and non-medical massage (OR = 0.3, 95% CI: 0.2-0.4, P < 0.001) were the effective independent influencing factors for the maximum breast abscess diameter > 5 cm. Conclusion: Low education , non-exclusive breastfeeding, fever >37.5℃, inverted or flat nipples, antibiotic used, and non-medical massage history have adverse effects on the enlargement of the abscess cavity with lactating breast abscesses patients.
Additional surgery rate after anterior or posterior cervical spinal surgery: Nationwide sample data analysis
Authors: Yuh Woon Tak , Kim Minjung , Choi Yunhee , Han Junghoon , Kim Junhoe , Kim Taeshin , Chung Chun Kee , Lee Chang-Hyun , Park Sung Bae , Kim Kyoung-Tae , Rhee John M. , Park Moon Soo , Kim Chi Heon 
Published: July 2022

Abstract
Surgical outcomes of degenerative cervical spinal disease are dependent on the selection of surgical techniques. Although a standardized decision cannot be made in an actual clinical setting, continued education is provided to standardize the medical practice among surgeons. Therefore, it is necessary to supervise and regularly update overall surgical outcomes. This study aimed to compare the rate of additional surgery between anterior and posterior surgeries for degenerative cervical spinal disease using the National Health Insurance Service-National Sample Cohort (NHIS-NSC) nationwide patient database. The NHIS-NSC is a population-based cohort with about a million participants. This retrospective cohort study included 741 adult patients (> 18 years) who underwent their first cervical spinal surgery for degenerative cervical spinal disease. The median follow-up period was 7.3 years. An event was defined as the registration of any type of cervical spinal surgery during the follow-up period. Event-free survival analysis was used for outcome analysis, and the following factors were used as covariates for adjustment: location of disease, sex, age, type of insurance, disability, type of hospital, Charles comorbidity Index, and osteoporosis. Anterior cervical surgery was selected for 75.0% of the patients, and posterior cervical surgery for the remaining 25.0%. Cervical radiculopathy due to foraminal stenosis, hard disc, or soft disc was the primary diagnosis in 78.0% of the patients, and central spinal stenosis was the primary diagnosis in 22.0% of them. Additional surgery was performed for 5.0% of the patients after anterior cervical surgery and 6.5% of the patients after posterior cervical surgery (adjusted subhazard ratio, 0.83; 95% confidence interval, 0.40–1.74). The rates of additional surgery were not different between anterior and posterior cervical surgeries. The results would be helpful in evaluating current practice as a whole and adjusting the health insurance policy.
Perception from Students regarding Online Synchronous Interactive Teaching in the Clinical Year during COVID-19 pandemic
Authors: Cheung Billy H.H. , Foo Dominic C.C. , Chu Kent Man , Co Michael , Lee Lok Sze 
Published: July 2022

Abstract
AimThe global pandemic of COVID-19 2020 has led to the extensive practice of online synchronous learning. Our primary goal is to compare different learning activities to evaluate students' perceptions. Moreover, we also aim to identify factors influencing their perception and satisfaction in these activities.MethodWe have divided the online activities into bedside teaching, practical skill sessions, tutorials or problem-based learning, and lectures. A questionnaire was distributed to our clinical year students to document their perceptions.Results Three hundred sixty-eight valid responses were received among these four learning activities combined (96 from bedside teaching, 67 from practical skill sessions, 104 from PBL/tutorial, and 101 from lecture).With the relative positive perception regarding online PBL and tutorials and preference for online lectures, there is a high possibility that these classes will remain online. To further improve their experience, students should turn on their videos during lectures. During online PBL and tutorials, good audio and video quality should always be ensured while being encouraged to remain their mic switched on. Online practical skill classes scored high in the DREEM-extracted questionnaire but were not preferred over face-to-face classes. Implementing online classes in this category should remain cautious with the limitations of tools, practice, and feedback. Good audio and video quality may help improve the experience. Conclusion The advantages of learning online may lead to a persistent practice of online teaching after this pandemic. This study provides insight and specific areas that need to improve.
A robust nasal tip location method based on template matching of three-scale FPFH local features
Authors: Yao Yu , Liang Wenhai 
Published: July 2022

Abstract
Nasal tip is an important feature point of human face, which is widely used in face recognition andface cutting. In this study, a new nasal tip location method is proposed to adapt to different posesfaces. The average effective energy and surface curvature are used to filter the point cloud to obtainthe set of facial convexities. On this basis, the set of facial convex points is divided into subsets ofdifferent facial organs using Density-Based Spatial Clustering of Applications with Noise(DBSCAN)clustering method. The points with the smallest euclidean distance between the geometric center ofthe subset and the original point cloud in the projection of the XOY plane are considered as candidatesfor nasal tip. To extract the local characteristics of nasal tip candidates,three-scale Fast Point FeatureHistogram (FPFH) is used. Finally, The point with the highest correlation obatained by calculatingPearson coefficient between artificially marked nasal tip and each nasal tip candidates is regarded asnasal tip. Experiments on CASIA-3D face databases with different faces and postures show that theaccuracy of nasal tip location reaches 94.8% when the locating error is 30 mm.And in addition toside face, the proposed method is also better than other advanced algorithms using local features.
A new approach to estimating parameters of the probability distribution in fuzzy data – an application to classifying Vietnamese Herb Leaf
Authors: Le Hoa , Bao Pham The , Vu Sang , Nhi Lam Thuy Le 
Published: July 2022

Abstract
The probability distribution of data are parameter-based and typically formatted as script numbers. However, these values areusually estimated experimentally, leading to selecting only one value is error-prone. Moreover, the real data often follows amixed form of probability distributions, in which sub-datasets are often incomplete data. It is possible to use parameters forensuring flexibility, especially in classification problems. Therefore, in this paper, we propose a new method for describingparameters estimated through Bayesian statistics; the new similarity between two probability distributions with the fuzzyparameters, through the fuzzy extended Kullback – Leibler divergence. The paper also applied the proposed methods in leaf classification.
Kinetics study and bio-phase characteristics of drainage in paddy fields purified by a compound ecological ditch
Authors: Zhang Wenyi , Huang Jinjie , Xu Mingchen , Mao Linqiang 
Published: July 2022

Abstract

Paddy field drainage discharged directly into natural waters without the treatment will aggravate water eutrophication. The removal characteristics of the purified paddy field drainage was explored by establishing an experimental device that simulates a compound ecological ditch (CED), which significantly optimized the absorption capacity of nutrients in rice fields. The results show that the changes of pollutants before and after the experiment indicates that the CED has a significant effect on the removal of pollutants in the drainage of the paddy field. The degradation trend of pollutants in 5 rice growth cycles is in agreement with the first-order kinetic degradation model. Bio-phase microscopy and microbial diversity index reveal that the species of bacteria and plankton algae increase in the continuous operation of the CED, accompanied by the emergence of higher micro-animals. It shows that improved ecosystem water quality is more suitable for the survival and reproduction of various microorganisms. The research results provided a new idea for further improving paddy field drainage treatment efficiency.
Engineering of Gold Nanorods as Multifunctional Theranostic Agent for Photothermal-Enhanced Radiotherapy of Cervical Cancer
Authors: Xie Lina , Ying Xia , Li Xinyue , Tan Xinyue , Zhang Tianzi , Zhang Xujia , Cai Wen , An Feifei , Liu Xiaoli , Han Suxia 
Published: July 2022

Abstract
Radiotherapy (RT) is an important method in the treatment of cervical cancer. Devising exceedingly effective, conveniently synthesizable, and lowly poisonous smart multifunctional radiosensitizers is of tremendous significance to improve the cure rate of RT. Gold nanorods (GNRs), which exhibited dramatic X-ray absorption and near-infrared (NIR) photothermal conversion effectiveness, could be utilized as radiosensitizers. In the present study, an all-in-one theranostic nanoagent for photothermal-enhanced radiotherapy of carcinoma was devised based on GNRs with aggregation-induced emission (AIE) attributes (NPAPF-GNRs@PEG). NPAPF-GNRs@PEG-mediated PTT-RT demonstrated excellent radiation sensitization effect by significantly inhibiting invasion and migration, increasing ROS and causing more DNA damage in two different pathological types of cell lines of cervical cancer. Experiments of in vitro and in vivo and metabolomics study demonstrated that PTT-RT based on NPAPF-GNRs@PEG showed enhanced RT effect by increasing apoptosis, compared to RT alone. Meanwhile, the NPAPF-GNRs@PEG nanoagent exhibited excellent tomography imaging (CTI)/photoacoustic imaging (PAI)/fluorescence imaging (FLI)/photothermal imaging (PTI) multi-modal imaging in vitro and in vivo. Therefore, the present study provides an innovative multifunctional platform for optimizing the curative efficacy of precision RT and further enriches the theoretical basis of the mechanistic study of radio-sensitization by GNRs-mediated PTT-RT.
Leveraging mHealth and a Milk Expression Frequency Biomarker During Postpartum to Prolong Lactation Among Parents of Critically Ill Infants: A Pilot Study
Authors: Magalhães Marina , Havelaar Arie , Varma Deepthi , Cacho Nicole , Sullivan Sandra , Parker Leslie 
Published: July 2022

Abstract
Objective: To assess the feasibility and potential benefits of personalized biomarker-based text messages in prolonging lactation among parents of critically ill infants. Study Design: Thirty-six participants were randomized to receive either daily texts with Mother’s Own Milk (MOM) sodium levels or standard care. Surveys at months 1 and 3 assessed whether infants were receiving exclusive MOM feeding, any MOM feeding, and whether the parent was still lactating. Kaplan-Meier and log-rank tests were used for survival analysis within and between intervention and control groups. Results: Participants were predominantly on Medicaid (72%), delivered infants < 1500g, and by c-section (56%). Survival probabilities at month 3 suggest prolonged MOM feeding (63% [.95CI, .43-.91] vs 41% [.95CI, .21-.67]) and lactation (63% [.95CI, .42-.95] vs 37% [.95CI, .18-.76]) in the enhanced group compared to the control group. Conclusion: Personalized biomarker-based text messages are feasible and may prolong lactation and MOM feeding among parents of critically ill infants.
Experimental Study of Expansive soil mixed with Kota Stone Slurry Powder
Authors: Rana Lakshmikant , Charan H.D. 
Published: July 2022

Abstract
In India, waste material disposal is a problem because lack of land and disposal techniques. Rajasthan is a big supplier of kota stone due to this large amount of kota stone slurry powder is produced. Kota stone slurry powder is a good inert filler material and a good stabilizing material, which improves the pore structure and enhance the resistance of the concrete to various harmful actions and mortars with pozzolanic and non-pozzolanic material. Kota stone slurry powder enhances durability properties. In this study we discuss the effect of adding kota stone slurry powder as a stabilizing agent and filler material into expansive soil. In this investigation kota stone slurry powder is mixed with expensive soil in different proportions and the effect of changes in parameters of expansive soil are recorded. In the present study, we discuss the effect of adding the kota stone slurry powder as a stabilizing agent and filler material into expansive soil. In this investigation kota stone slurry powder is mixed with expansive soil in different proportions and the effect of changes in parameters of expansive soil are recorded in the form of tables and graphs.