bluuebunny/crossref_metadata_2025_split huggingface dataset:
Are Technical Skills Assessed Using Medical Knowledge Associated with Non-technical Skill Knowledge in Anaesthesia Resident Training?
Authors: Oofuvong Maliwan , Pattaravit Ngamjit , Kanjanawanichkul Orarat , Siripruekpong Sirikarn , Nuanjun Kanjana , Suwannarat Boonthida 
Published: December 2022

Objective: We examined the association between midterm examinations and anaesthetists’ non-technical skills (ANTS) knowledge using multiple choice questions (MCQs) for anaesthesia training.
Materials and Methods: A prospective cross-sectional study was implemented based on two cohort studies conducted in November 2017 and November 2019 at the university hospital in southern Thailand. Each cohort consisted of MCQs, short answer questions (SAQs), objective structured clinical examinations (OSCEs), and MCQs after ANTS simulation workshops during the midterm examinations. The main exposure variable was the midterm examination (MCQs/ SAQs/ OSCEs) whereas the ANTS MCQs were the outcome. The potential predictors were the residents’ role, sex, PGY (1-3), and competency. Associations between midterm examinations and ANTS MCQ scores were analysed using Pearson’s correlation coefficients (r) and multivariate linear regression analysis, and presented as beta coefficient (β) and 95% confidence limit (CL).
Results: Forty-eight anaesthesia residents were recruited for the study. After adjusting for PGY, knowledge score, and attitude evaluated by the staff, OSCE was found to be significantly associated with the pretest ANTS scores (β [95% CL]= 1.02 (0.06, 1.98)) and MCQ scores were significantly associated with the posttest ANTS scores (β [95% CL]= 0.14 (0.04, 0.24)). SAQ scores had negative associations with the pretest (β [95% CL]=-0.11 (-0.21, -0.01)) and posttest (β [95% CL]=-0.16 (-0.27, -0.05)) ANTS scores. It was found that there was a significant relationship between midterm examinations and posttest ANTS scores (r=0.52).
Conclusion: Technical skills using OSCE and midterm MCQ examination scores were associated with non-technical skill knowledge in anaesthesia training.
Herramienta para revisión digital y captación de datos de proyectos de construcción
Authors: Rojas Henrry , Yaguana Axel , Baculima Francisco 
Published: December 2022

La construcción informal genera incertidumbre de comportamiento estructural de los proyectos, que a su vez implican vulnerabilidad frente a fuentes de peligro como los terremotos. Una de las razones de la proliferación de la informalidad en la industria de la construcción es la falta de regulación y control. Las instituciones a cargo de los procesos de revisión de diseño, aprobación y supervisión de proyectos de construcción poseen limitaciones de recursos económicos y humanos para el desarrollo de sus funciones. Así, es necesario optimizar procesos con el fin de mejorar las labores de regulación. Una de las etapas críticas para el control de cumplimiento normativo es la aprobación de diseños estructurales. El presente trabajo tiene como objetivo desarrollar una plataforma de código abierto que permita crear, supervisar, aprobar y almacenar datos e información de diseños estructurales. A través del uso de tecnologías frontend: HTML, CSS y JavaScript se configuró la plataforma virtual. Los inputs requeridos para la creación de los informes estructurales pretenden que los informes generados sean concisos para facilitar las labores de revisión y almacenamiento de datos. La plataforma fue evaluada y calificada positivamente por un grupo de profesionales del área de la ingeniería estructural.
Cancer epigenetics in clinical practice
Authors: Davalos Veronica , Esteller Manel 
Published: December 2022

AbstractCancer development is driven by the accumulation of alterations affecting the structure and function of the genome. Whereas genetic changes disrupt the DNA sequence, epigenetic alterations contribute to the acquisition of hallmark tumor capabilities by regulating gene expression programs that promote tumorigenesis. Shifts in DNA methylation and histone mark patterns, the two main epigenetic modifications, orchestrate tumor progression and metastasis. These cancer‐specific events have been exploited as useful tools for diagnosis, monitoring, and treatment choice to aid clinical decision making. Moreover, the reversibility of epigenetic modifications, in contrast to the irreversibility of genetic changes, has made the epigenetic machinery an attractive target for drug development. This review summarizes the most advanced applications of epigenetic biomarkers and epigenetic drugs in the clinical setting, highlighting commercially available DNA methylation‐based assays and epigenetic drugs already approved by the US Food and Drug Administration.
Pengendalian Waktu Pembangunan Jalan Poros Desa Ruas Kedungprahu- Sukowiyono
Authors: Supriyono Lyya , Fatmawati Laily 
Published: December 2022

Abstractâ€” The road is a vital tool for the village community where the majority of their livelihood is as a farmer. Increasing the economy in the village is not only determined from agricultural yields but also determined by supporting facilities and infrastructure such as road repair. The road construction process with a long duration of time will certainly hamper the performance of the farmers which has an impact on declining income. Therefore it is necessary to control the working time on the Construction of the Village Axis Road Kedungprahu-Sukowiyono so that work can be completed as quickly as possible without reducing the quality of road pavement work. In this study we use the CPM (Critical Parth Method) method in evaluating the scheduling of the Kedungprahu-Sukowiyono road segment construction project. With the CPM (Critical Parth Method) Method we can find out the critical paths and non-critical paths. The results of the study of controlling the time of construction of the village road axis of the Kedungprahu-Sukowiyono section were accelerated on the critical path. Savings of funds amounted to 1,723,879.68, there was no change in the critical path after accelerating the project with the addition of overtime hours, the implementation time from 60 days to 55 days.Â Keywordsâ€”: Time control; road; kedungprahu.
Tumor venéreo transmissível extragenital primário com metástase esplênica: relato de caso clínico
Authors: Gundim Lígia Fernandes , Souza Rafael Rocha de , Coleto Arlinda Flores , Moreira Thaís de Almeida , Tivelli Patrícia , Medeiros-Ronchi Alessandra Aparecida 
Published: December 2022

O tumor venéreo transmissível (TVT) é uma neoplasia que acomete predominantemente a genitália dos cães, mas a apresentação exclusivamente extragenital já foi observada. A transmissão ocorre por implantação de células tumorais por contato. Os animais adultos e com acesso à rua geralmente são os mais acometidos. TVT genitais raramente apresentam metástases e ainda não havia sido relatado metástase de TVT exclusiviamente extragenital. A PAAF (punção por aspiração com agulha fina) é o método padrão utilizado para o diagnóstico. O objetivo do presente relato foi descrever os achados clínicos, citopatológicos, histopatológicos e imunohistoquímicos de um caso de TVT cutâneo com metástase esplênica, sem presença de lesão genital, em uma cadela de quatro anos de idade, atendida no Hospital Veterinário da Universidade Federal de Uberlândia. Ao exame clínico foram observados múltiplos nódulos subcutâneos. A PAAF de todos os nódulos foi realizada. Ao exame de ultrassom da região abdominal, foi observado aumento do baço. O animal foi submetido à cirurgia de esplenectomia. Fragmentos da massa foram enviados para o laboratório de histopatologia do Hospital Veterinário da Universidade Federal de Uberlândia para serem processados rotineiramente. Devido à localização incomum do tumor no baço, foi realizado painel imunohistoquímico para o diagnóstico diferencial. As células neoplásicas foram positivas para o anticorpo anti-vimentina, anti-lisozima e alfa1-antitripsina. A localização primária cutânea de TVT é infrequente e metástase ainda não havia sido relatada. Sendo assim, a confirmação imunohistoquímica é aconselhável nestes casos.
Efficient removal of tetracycline from aqueous solution by K2CO3 activated penicillin fermentation residue biochar
Authors: Liu Yanfang , Gao Wei , Yin Sijie , Liu Rui , Li Zaixing 
Published: December 2022

In this study, biochar was prepared using penicillin fermentation residue (PR) as the raw material by different methods. The adsorption behavior and adsorption mechanism of biochar on tetracycline (TC) in an aqueous environment were investigated. The results showed that K2CO3 as an activator could effectively make porous structures, and that biochar with mesoporous or microporous could be prepared in a controlled manner with two kinds of different activation methods, the dry mixing method and the impregnation method. The dry mixing method could create more mesopores, while the impregnation method could prepare more micropores. Microporous biochar (IKBCH) with a high specific surface area could be prepared by the impregnation method combined with HCl soaking, which has an excellent adsorption effect on tetracycline. When the concentration of tetracycline was 200 mg/L, the removal rate of 99.91% could be achieved with the dosage of microporous biochar at 1 g/L. The adsorption process was in accordance with the Langmuir model and the pseudo-second-order model, respectively. The maximum adsorption capacity of IKBCH was 268.55 mg/g (25°C). The adsorption mechanisms were pore filling, π-π interaction, electrostatic adsorption, and hydrogen bond. Its stable and wide applicability adsorption process does not cause ecological pollution in the aqueous environment, and it is a promising biochar adsorbent.
Design, synthesis and biological evaluation of a novel colchicine-magnolol hybrid for inhibiting the growth of Lewis lung carcinoma in Vitro and in Vivo
Authors: Li Zhiyue , Hu Shengquan , Pu Liu-Yang , Li Ziwen , Zhu Guanbao , Cao Yongkai , Li Limin , Ma Yucui , Liu Zhanyan , Li Xinping , Liu Guangjie , Chen Keji , Wu Zhengzhi 
Published: December 2022

Colchicine is a bioactive alkaloid originally from Colchicum autumnale and possesses excellent antiproliferative activity. However, colchicine-associated severe toxicity, gastrointestinal side effects in particular, limits its further therapeutic use. In the current study, we thus designed and synthesized a novel hybrid (CMH) by splicing colchicine and magnolol, a multifunctional polyphenol showing favorable gastrointestinal protection. The antitumor activity of CMH in Lewis lung carcinoma (LLC) was then evaluated in vitro and in vivo. Biologically, CMH inhibited the growth of LLC cells with an IC50 of 0.26 μM, 100 times more potently than cisplatin (26.05 μM) did. Meanwhile, the cytotoxicity of CMH was 10-fold lower than that of colchicine in normal human lung cells (BEAS-2B). In C57BL/6 mice xenograft model, CMH (0.5 mg/kg) worked as efficacious as colchicine (0.5 mg/kg) to inhibit tumor growth and 2 times more potently than cisplatin (1 mg/kg). In terms of mortality, 7 out of 10 mice died in colchicine group (0.75 mg/kg), while no death was observed in groups receiving CMH or cisplatin at 0.75 mg/kg. Mechanistic studies using Western blot revealed that CMH dose-dependently suppressed the protein expression of phosphorylated ERK. Molecular docking analysis further indicated that CMH was well fitted in the colchicine binding site of tubulin and formed several hydrogen bonds with tubulin protein. These results enable our novel hybrid CMH as a potential antineoplastic agent with lower toxicity, and provide perquisites for further investigation to confirm the therapeutic potentiality of this novel hybrid.
Identification and validation of senescence-related genes in circulating endothelial cells of patients with acute myocardial infarction
Authors: Xiang Jie , Shen Jun , Zhang Ling , Tang Baopeng 
Published: December 2022

BackgroundAcute myocardial infarction (AMI) is the main clinical cause of death and cardiovascular disease and thus has high rates of morbidity and mortality. The increase in cardiovascular disease with aging is partly the result of vascular endothelial cell senescence and associated vascular dysfunction. This study was performed to identify potential key cellular senescence-related genes (SRGs) as biomarkers for the diagnosis of AMI using bioinformatics.MethodsUsing the CellAge database, we identified cellular SRGs. GSE66360 and GSE48060 for AMI patients and healthy controls and GSE19322 for mice were downloaded from the Gene Expression Omnibus (GEO) database. The GSE66360 dataset was divided into a training set and a validation set. The GSE48060 dataset was used as another validation set. The GSE19322 dataset was used to explore the evolution of the screened diagnostic markers in the dynamic process of AMI. Differentially expressed genes (DEGs) of AMI were identified from the GSE66360 training set. Differentially expressed senescence-related genes (DESRGs) selected from SRGs and DEGs were analyzed using Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and protein-protein interaction (PPI) networks. Hub genes in DESRGs were selected based on degree, and diagnostic genes were further screened by gene expression and receiver operating characteristic (ROC) curve. Finally, a miRNA-gene network of diagnostic genes was constructed and targeted drug prediction was performed.ResultsA total of 520 DEGs were screened from the GSE66360 training set, and 279 SRGs were identified from the CellAge database. The overlapping DEGs and SRGs constituted 14 DESRGs, including 4 senescence suppressor genes and 10 senescence inducible genes. The top 10 hub genes, including FOS, MMP9, CEBPB, CDKN1A, CXCL1, ETS2, BCL6, SGK1, ZFP36, and IGFBP3, were screened. Furthermore, three diagnostic genes were identified: MMP9, ETS2, and BCL6. The ROC analysis showed that the respective area under the curves (AUCs) of MMP9, ETS2, and BCL6 were 0.786, 0.848, and 0.852 in the GSE66360 validation set and 0.708, 0.791, and 0.727 in the GSE48060 dataset. In the GSE19322 dataset, MMP9 (AUC, 0.888) and ETS2 (AUC, 0.929) had very high diagnostic values in the early stage of AMI. Finally, based on these three diagnostic genes, we found that drugs such as acetylcysteine and genistein may be targeted for the treatment of age-related AMI.ConclusionThe results of this study suggest that cellular SRGs might play an important role in AMI. MMP9, ETS2, and BCL6 have potential as specific biomarkers for the early diagnosis of AMI.
The prognostic capability of inflammatory proteins in predicting peripheral artery disease related adverse events
Authors: Li Ben , Djahanpour Niousha , Zamzam Abdelrahman , Syed Muzammil H. , Jain Shubha , Arfan Sara , Abdin Rawand , Qadura Mohammad 
Published: December 2022

BackgroundLevels of inflammatory proteins and their prognostic potential have been inadequately studied in patients with peripheral artery disease (PAD). In this study, we quantified and assessed the ability of inflammatory proteins in predicting PAD-related adverse events.MethodsIn this prospective case-control study, blood samples were collected from patients without PAD (n = 202) and patients with PAD (n = 275). The PAD cohort was stratified by disease severity based on ankle brachial index (ABI): mild (n = 49), moderate (n = 164), and severe (n = 62). Patients were followed for 2 years. Plasma concentrations of 5 inflammatory proteins were measured: Alpha-2-Macroglobulin (A2M), Fetuin A, Alpha-1-Acid Glycoprotein (AGP), Serum Amyloid P component (SAP), and Adipsin. The primary outcome of our study was major adverse limb event (MALE), defined as the need for vascular intervention (open or endovascular revascularization) or major amputation. The secondary outcome was worsening PAD status, defined as a drop in ABI greater than or equal to 0.15 over the study period. Multivariable logistic regression was performed to assess the prognostic value of inflammatory proteins in predicting MALE, adjusting for confounding variables.ResultsCompared to patients without PAD, three inflammatory proteins were differentially expressed in patients with PAD (AGP, Fetuin A, and SAP). The primary outcome (MALE) and secondary outcome (worsening PAD) status were noted in 69 (25%) and 60 (22%) patients, respectively. PAD-related adverse events occurred more frequently in severe PAD patients. Based on our data, the inflammatory protein AGP was the most reliable predictor of primary and secondary outcomes. On multivariable analysis, there was a significant association between AGP and MALE in all PAD disease states [mild: adjusted HR 1.13 (95% CI 1.05–1.47), moderate: adjusted HR 1.23 (95% CI 1.16–1.73), severe: adjusted HR 1.37 (95% CI 1.25–1.85)]. High levels of AGP were associated with lower 2-year MALE-free survival in all PAD disease states [mild (64% vs. 100%, p = 0.02), moderate (64% vs. 85%, p = 0.02), severe (55% vs. 88%, p = 0.02), all PAD (62% vs. 88%, p = 0.01)].ConclusionLevels of inflammatory protein AGP may help in risk stratifying PAD patients at high risk of MALE and worsening PAD status and subsequently facilitate further vascular evaluation and initiation of aggressive medical/surgical management.
Reconstruction of submarine eruption processes from FTIR volatile analysis of marine tephra: Example of Oomurodashi volcano, Japan
Authors: McIntosh Iona M. , Aoki Kaori , Yanagishima Taiki , Kobayashi Makoto , Murata Masanori , Suzuki Takehiko 
Published: December 2022

Tephra layers in marine sediments are widely used to correlate and date paleoclimate and paleoceanography records, and to determine spatiotemporal changes in magmatic evolution and eruption frequency. Dissolved matrix glass H2O contents of marine tephra could potentially inform understanding of eruption processes but are rarely used due to the issue of secondary hydration after deposition. Recent advancements in Fourier transform infrared spectroscopy (FTIR) volatile analysis have enabled reconstruction of original water contents of hydrated volcanic glasses. These new Fourier transform infrared spectroscopy analysis methods offer a new way to investigate tephra stored in marine sedimentary archives. We present a case study of the Od-1 tephra layer in marine sedimentary core C9010E, drilled ∼40 km south of the Boso peninsula in Japan. This tephra was erupted by the shallow silicic submarine Oomurodashi volcano in the northern Izu-Bonin arc at ∼13.5 ka. Our Fourier transform infrared spectroscopy volatile data show it has been affected by secondary hydration, with the extent of hydration controlled by grain size and porosity characteristics. Numerical modelling of low temperature hydration suggests Fourier transform infrared spectroscopy data offer an additional method for estimating eruption ages of marine tephra. OH contents, unaltered by low temperature secondary hydration, record low ambient eruptive pressures for all grain sizes and tephra types i.e., blocky and dense or pumiceous. Consideration of hydrostatic pressure gradients and past sea level at Oomurodashi shows that the majority of tephra volatile data cannot be explained by quench within a submarine eruption plume. Instead, OH contents record quench fragmentation within the shallow submarine edifice. Physical characteristics of the tephra are consistent with the formation of these tephra by explosive phreatomagmatic eruption processes. Together these OH data and tephra characteristics support the interpretation that the Od-1 tephra layer was formed by the same shallow phreatomagmatic eruption that formed the existing Oomuro Hole crater and that produced subaerial tephra deposits on nearby Izu-Oshima and Toshima islands. This study demonstrates the crucial contribution that imaging Fourier transform infrared spectroscopy analysis can make to the interpretation of degassing and eruption processes of volcanic glasses, particularly vesicular pyroclasts and/or glasses affected by secondary hydration, adding an important new dimension to marine tephra research.
The concentrations of bone calcium, phosphorus and trace metal elements in elderly patients with intertrochanteric hip fractures
Authors: Lin Shangjin , Chen Cong , Cai Xiaoxi , Yang Fengjian , Fan YongQian 
Published: December 2022

IntroductionTrace metal elements may play a crucial role in bone mineralization and metabolism. However, the quantification of trace element concentrations in human bone tissue has received little attention.Materials and methodsBone tissue samples were collected from 55 elderly patients (15 males and 40 females) with intertrochanteric hip fractures. The calcium, phosphorus, manganese, iron, copper, and zinc concentrations in the cortical bone zone, cancellous bone zone, and junction zone between cortical and cancellous bone were determined by energy-dispersive X-ray fluorescence (EDX). The differences in trace element concentrations in the three regions were compared, and the correlation between gender and bone trace element contents of the bones was analyzed using the Kruskal-Wallis’s test. The correlation between age, body mass index (BMI), and bone calcium, phosphorus concentrations, and trace elements in three bone zones was determined using Spearman correlation analysis.ResultsThe Kruskal-Wallis test showed no difference in bone phosphorus concentration among the three regions. In contrast, the difference in the concentrations of bone calcium and four metal elements was statistically significant (P&lt;0.01). In addition, no statistical differences were observed in the concentrations of trace elements among the three regions in elderly male and female patients. Spearman correlation analysis showed a strong negative correlation between bone calcium and phosphorus in three bone regions (r=-0.999, -0.95, -0.998, P &lt; 0.01) and a significant positive correlation between trace metal elements in the cancellous bone zone. In the junction zone, the BMI showed a strong positive correlation with bone calcium content (r=0.347, P=0.009) and a significant negative correlation with phosphorus content (r=-0.349, P=0.009).ConclusionBone calcium and phosphorus were the main components of hydroxyapatite, and these two elements accounted for the majority of bone mineral salts. Trace metal elements are essential for bone metabolism and specific synergistic interactions. BMI may be associated with bone calcium and phosphorus contents in elderly patients with osteoporosis.