bluuebunny/crossref_metadata_2025_split huggingface dataset:
Favorable Locoregional Control in Clinically Node- Negative Hormone-receptor Positive Breast Cancer With Low 21-gene Recurrence Scores: a Single Institutional Study With 10-year Follow-up
Authors: Uras Cihan , Cabioglu Neslihan , Tokat Fatma , Er Ozlem , Kara Halil , Korkmaz Taner , Bese Nuran , Ince Umit 
Published: July 2022

Abstract
Background: Recent studies have shown a lower likelihood of locoregional recurrences in patients with low 21-gene recurrence score (RS). In this single institutional study, we investigated any associations between different cut-off values of 21-gene RS and histopathological factors and outcome in patients with long-term follow-up. Methods: Between February 2010 to February 2013, 61 patients with early stage clinically node-negative hormone receptor-positive and HER2-negative breast cancer tested for the 21-gene RS assay, were included into the study. Demographic, clinicopathological, treatment and outcome characteristics were analyzed.Results: Median age was 48 (range, 29-72). Of those, 53 patients were diagnosed with Stage 1 (86.9%) and 8 patients were Stage 2 (13.1%) following surgery. Patients with high histologic grade (HG), or with Ki-67>25%were more likely to have intermediate/high RS based on RS >11 and >18. Based on the 21-gene RS assay, only 19 patients (31%) received adjuvant chemotherapy. At a median follow-up of 112 months, 3 patients developed local recurrences (4.9%) treated with endocrine therapy alone. Among patients treated with endocrine treatment alone (n=42), the clinicopathological characteristics including age <40, age <50, high histologic or nuclear grade, high Ki67-scores (>20%, >25%, >30%), presence of lymphovascular invasion, luminal-A type, multifocality, lymph node positivity, tumor size more than 2 cm, RS>18 or RS>11 were not significantly found to be associated with 10-year locoregional recurrence free survival (LRRFS). However, patients with a RS >16 have significantly shown a poorer 10-year LRRFS compared to those with RS <16 (RS <16; 100% vs RS>16; 75%, p=0.039). Conclusions: These results suggest that Oncotpe DX assay may be of little value in patients with high histologic grade or high Ki67 scores (>25%) as treatment decision criteria to determine any benefit from chemotherapy. Furthermore, patients with a RS >16 are more likely to benefit from adjuvant chemotherapies, whereas those with a RS <16 have an excellent outcome and local control on long term follow-up with endocrine treatment alone.
Identification and functional exploration of Ferroptosis and Immune Related Long Non-Coding RNA in Inflammatory bowel disease
Authors: Hu Yunhong , Sun Shaopeng , Li Heng 
Published: July 2022

Abstract
Background：Inflammatory bowel disease (IBD) is an idiopathic intestinal inflammatory disease involving ileum, rectum and colon, including ulcerative colitis (UC) and Crohn's disease (CD).The etiology is complex, and immune dysfunction is considered to be the final pathological result. Methods：We constructed a lncRNA-mRNA regulatory network based on ferroptosis-related and immnue-related differentially expressed lncRNAs in IBD. Results: We identified 6 differently expressed lncRNAs associated with of ferroptosis and immune in IBD. We further constructed lncRNA-mRNA regulatory network and found potential miRNAs and transcription factors (TF) based on trans and ceRNAs mechanisms.Conclusion：Ferroptosis and immune related lncRNA participate in the regulation of abnormal immune response in IBD
Clinically relevant benzoxaboroles inhibit mRNA processing in Trypanosoma brucei
Authors: Waithaka Albina , Clayton Christine 
Published: July 2022

Abstract
Objective
The cleavage and polyadenylation endonuclease CPSF73 is thought to be the target of the anti-trypanosomal benzoxaboroles AN7973, acoziborole and AN11736. We previously showed that AN7973 inhibits mRNA processing. We here investigated whether the drug candidates acoziborole (for human sleeping sickness) and AN11736 (for nagana in cattle) have same effect. We also affinity purified tagged CPSF73 from parasites without, or after, AN7973 treatment, and analysed differentially co-purified proteins my mass spectrometry.
Results
AN11736 and acoziborole both inhibited mRNA processing, as demonstrated by decreased levels of spliced mRNAs and accumulation of di- and tri-cistronic mRNAs from the alpha-beta tubulin locus. Treating the cells with AN7973 did not significantly affect the proteins that copurified with CPSF73.
Detection of second primary lymphoma in late diffuse large B-cell lymphoma recurrences
Authors: Berendsen Madeleine , Bladel Diede van , Hesius Eva , de Groot Fleur , Kroeze Leonie , Rijntjes Jos , Luijks Jeroen , Hoevenaars Brigiet , Halilovic Altuna , Nooijen Peet , Spek Ellen van der , Vermaat Joost , Hess Corine , Hebeda Konnie , Stevens Wendy , Krieken J. Han van , Brand Michiel van den , Groenen Patricia , Scheijen Blanca 
Published: July 2022

Abstract
Approximately one-third of diffuse large B-cell lymphoma (DLBCL) patients relapse, who often require salvage chemotherapy followed by autologous stem cell transplantation. In most cases, the clonal relationship between the first diagnosis and subsequent relapse is not assessed, thereby missing the identification of second primary lymphoma. Here, the clonal relationship of 59 paired DLBCL diagnosis and recurrences was established by next-generation sequencing (NGS)-based detection of immunoglobulin gene rearrangements. In 50 cases with interpretable results, 43 DLBCL patients (86%) developed clonally related lymphoma and relapsed disease. In total, 100% of early recurrences (< 2 year), 80% of the recurrences with an interval between 2 and 5 years, and 73% of late recurrences (≥ 5 year) were clonally related. In contrast, 7 out of 50 patients (14%) showed distinct dominant clonotypes in primary DLBCL and its recurrences, suggesting the occurrence of second primary DLBCL, which all occurred at least 4 years after primary diagnosis. Moreover, 43% of clonally unrelated cases were Epstein-Barr virus-positive, while this was 3% in relapsed DLBCL. In conclusion, NGS-based clonality testing in late recurrences should be considered in routine diagnostics to distinguish relapse from second primary lymphoma, as this latter group of DLBCL patients may benefit from less intensified treatment strategies.
Microglia Sirt6 modulates transcriptional activity of NRF2 to defend high fat diet-induced obesity
Authors: Xiao Xiaoxia , Hu Huiling , Zhong Yadi , Chen Yingjian , Tang Kaijia , Pan Zhisen , Huang Jiawen , Yang Xiaoying , Wang Qi , Gao Yong 
Published: July 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Varidnaviruses in the human gut: a major expansion of the order Vinavirales
Authors: Yutin Natalya , Rayko Mike , Antipov Dmitry , Mutz Pascal , Wolf Yuri I , Krupovic Mart , Koonin Eugene V 
Published: July 2022

Abstract
Background
Bacteriophages play key roles in the dynamics of the human microbiome. By far the most abundant components of the human gut virome are tailed bacteriophages of the realm Duplodnaviria, in particular, crAss-like phages. However, apart from duplodnaviruses, the gut virome has not been dissected in detail.
Results
Here we report a comprehensive census of a minor component of the gut virome, the tailless bacteriophages of the realm Varidnaviria. Tailless phages are primarily represented in the gut by prophages of the families Corticoviridae and Autolykiviridae that jointly comprise the order Vinavirales and are mostly integrated as prophages in genomes of Alphaproteobacteria and Verrucomicrobia. Phylogenetic analysis of the major capsid proteins (MCP) and packaging ATPases suggests that at least three new families within Vinavirales should be established to accommodate the diversity of prophages from the human gut virome. Previously, only the MCP and ATPase genes were reported to be conserved in all members of Vinavirales. Here we identify a core set of 12 proteins that are shared by most of these viruses including previously undetected lysis enzymes. We further demonstrate that replication system components are frequently replaced in the genomes of Vinavirales, suggestive of selective pressure for escape from yet unknown host defenses or avoidance of incompatibility with coinfecting related viruses.
Conclusions
The results of this analysis show that, in a sharp contrast to marine viromes, varidnaviruses are a minor component of the human gut virome. Moreover, they are primarily represented by proviruses, suggesting that there are few if any active varidnavirus infections in the gut at any given time. These findings complement the existing knowledge of the human gut virome by exploring a group of viruses that was virtually overlooked in previous work.
Anticancer and Apoptotic Activity in Neuroblastoma SK-N-SH using Phospholipid Extract from Bone of Scomberomorus niphonius
Authors: Kiddane Anley Teferra , Roy Vikash Chandra , Kang Min-Jae , Patil Maheshkumar Prakash , Chun Byung-Soo , Kim Gun-Do 
Published: July 2022

Abstract
Among various types children’s health challenges, Neuroblastoma is the most serious solid neoplasm forming outside the cranium. S. niphonius is a valuable edible fish that has been widely used for a meal. In this study, we obtained phospholipid extract from the bone of S. niphonius with the supercritical CO2 extraction method and tested anticancer activity with a cell viability assay. The phospholipid showed anticancer activity on neuroblastoma SK-N-SH cells, and the anticancer activity was presented with an IC50 of 710.25 ± 28.31 µg/ml, but didn’t show a significant toxicity on HUVEC cell lines. Westernblot was used to detect signaling proteins; Bak, caspase-9, caspase-8, caspase-3, Bax and IκBα were increased, whereas IKKβ and NFκB were downregulated in experimental groups compared to untreated groups. Gene expression was revealed by RT-qPCR, and the fold ratio of Apaf-1, cytochrome-c, caspase-9, caspase-3 and Bax genes’ expression was raised in treated groups, implying apoptosis. Gel electrophoresis revealed that the experimental groups had more fragmented DNA than the control group. The study shows that a phospholipid extract from S. niphonius’s bone could be used as a biological origin of anticancer activity in neuroblastoma SK-N-SH cells.
Machine learning and fuzzy logic application in predicting the effect of dynamic loading on rectangular armed panels - a comparison on impact loading
Authors: Tafarroj M. M. , Hatami H. 
Published: July 2022

Abstract
In a comprehensive experimental work, using of four basic mix designs, 64 rectangular composite panels were made with 100mm2 of area and 30, 45, 60, and 75mm thickness and tested by impact loading. Compressive, tensile, and flexural strength tests were performed on all the four max designs. Steel fibers with percentages of 0, 0.25, 0.5, and 0.75 with 25m of length were used to make the concrete composites. A hammer with 180kg weight and 7500J power was used as the impact loading with drop hammer test machine (DH-TM). The specimens were dynamically loaded by drop test from a 60cm height. The using of steels fibers and expanded sheets in combination with each other significantly increase the energy absorption. Moreover, the initial peak force increases, while crushing length and deformation of the specimens reduce. In the remainder of the study, using the data obtained from experiments, models based on two well-known techniques-namely artificial neural networks (ANN) and adaptive network-based fuzzy inference system (ANFIS) have been developed to construct a model for the problem. The performance of both techniques is compared and finally the most appropriate one for predicting new data is introduced.
Estimating the impacts of environmental management on strawberry yield using publicly available agricultural data in South Korea
Authors: Kim Steven , Jo Jung Su , Luk Vicky , Kim Sung Kyeom , Kim Dong Sub 
Published: July 2022

Abstract
Advanced information and communication technologies have made data collection more efficient, and crop growth, yield, and environmental data of multiple farms can be used for various purposes. Using the publicly available database in South Korea, we investigated the correlation between the management of greenhouse environments and the pattern of strawberry yield during harvest seasons. Location-specific datasets were merged and processed, and mixed-effects models were applied to account for both observable greenhouse conditions and unobserved factors associated with farm locations. Instead of using averaged temperature and relative humidity inside greenhouses, we estimated the percent of time that air temperature has been between 15°C and 20°C, denoted by T%, and the percent of time that relative humidity has been between 0% and 50%, denoted by H%. The strawberry yield tends to reduce with respect to time during a harvest season, but the reduction rate is slower when the T% and H% are higher. This study provides empirical evidence for the practical significance of maintaining air temperature and relative humidity within an optimal range, and we conclude that growers can mitigate loss of production by the maintenance of air temperature and relatively humidity particularly during the later phase of a harvest season.
Molecular Detection of Spotted Fever Group Rickettsiae and Ehrlichia in Rodents from Anhui Province, China
Authors: ADAMS ABDUL LATIF , BO-YU Liu , ZHEN CHEN , OBIRI-YEBOAH DORCAS , LIU YAN 
Published: July 2022

Abstract
BackgroundRickettsioses are important zoonotic diseases worldwide. Rickettsia are naturally maintained in vertebrate hosts and can emerge or reemerge with serious public health implications. Rodents are important host animals for many rickettsial pathogens. In China, several species of rickettsia have been reported in tick species, and few have been reported in vertebrate host animals, including rodents. Epidemiological studies that investigate natural foci and the role of small mammals in the transmission of zoonoses to humans are still needed. Thus, we investigated the presence of rickettsial agents in rodents from Anhui Province, China.MethodsA total of 143 rodents, including 131 Apodemus agrarius and 12 Niviventer niviventer, were captured alive using cage traps in the fields of four areas in Anhui Province. Tissue samples were collected aseptically from each rodent. DNA samples from rodent tissues were amplified by PCR based on rickettsial genes: the 17-kilodalton antigen gene (17-kDa), 16S rRNA gene (rrs) and citrate synthase gene (gltA). Positive amplicons were sequenced and confirmed by BLAST analysis. Phylogenetic analysis was performed by Mega 7.0 software.ResultsThe overall prevalence rate of rickettsial agents in rodents was 11.9% (17/143). The genes encoding the common rickettsial 17-kDa, gltA, and 16S rRNA were positively detected in 2.1% (3/143), 5.6% (8/143) and 4.2% (6/143) of the rodents, respectively, by nested PCR. The rickettsial agents detected included Rickettsia raoultii, Rickettsia japonica and Ehrlichia chaffeensis according to BLAST and phylogenetic analysis.ConclusionThis study demonstrates the presence of rickettsial agents in rodents captured in Anhui Province, China. Further epidemiological studies should be investigated in arthropod vectors, host animals and humans to estimate the impact of rickettsia infestations among rodents from these areas.
Supine Paediatric Percutaneous Nephrolithotomy: single centre experience with selective tubeless approach
Authors: Taghavi Kiarash , Pirpiris Athina , Webb Nathalie , Coleman Robert , McCahy Philip 
Published: July 2022

Abstract
Objective: Advances in percutaneous nephrolithotomy (PCNL) toward less invasive treatment options and miniaturisation of equipment have particular application in paediatric patients. The current study aims to establish the safety and efficacy of the modified-supine position in children and a selective “tubeless” approach to paediatric PCNL.Methods: A retrospective cohort study was performed of all paediatric patients undergoing PCNL at our tertiary institution over 10 years (2010-2019).Results: Of 635 PCNL patients, 20 were paediatric (3%). Staghorn calculi (complete or partial) made up 35% of the population. The majority of procedures were performed in the modified-supine position (85%). Median procedure time was 2.1 hours, and length of stay was 2.2 days. With regards post-operative drainage, a ureteric stent was not placed in 75% of cases. The stone free rate was 65%. The median follow-up time was 8.7 years (IQR 6.7-9.9 years).Conclusion: As a result of technical and technological advances, PCNL can be considered a safe and effective procedure in children with a large stone burden, in situations of anatomic complexity or when immediate stone-free status is required. Selectively reducing post-operative drainage and utilising a modified supine position is safe, efficacious and may provide additional benefits in children.
Materialistic happiness menaces people’s meaning in life: A serial double mediation model reflecting the roles of basic psychological needs satisfaction and subjective well-being
Authors: Lo On-Ting , Cheung Sing-Hang , Lai Veronica Ka Wai 
Published: July 2022

Abstract
Background: People are holding stronger materialistic belief than before, especially in some developed regions in the world. Materialism refers to beliefs that equate materialistic possessions with happiness, success, and make it central to life. Extant research has widely shown that materialism threatens people’s well-being. Nevertheless, the evidence on how materialism influences meaning in life is less well-established. Within the realm of this area, we examined the relationship between materialism and meaning in life, as well as its potential mechanisms, under the framework of Self-Determination Theory.Methods: Two cross-sectional online survey studies have been conducted to test the hypothesized serial double mediation model. In this model, we measured four constructs including people’s materialistic values, basic psychological needs satisfaction, subjective well-being, and meaning in life. In Study 1, we collected data from a Chinese sample (N=190). In Study 2, we tried to replicate the findings from Study 1 by collecting a non-Chinese different-age Prolific sample (N=767).Results: The results from Study 1 and Study 2 are consistent. We found that the relationship between materialistic happiness and meaning in life is serially mediated by basic psychological needs satisfaction and subjective well-being. The results confirm that materialistic happiness frustrates basic psychological needs, which threatens people’s subjective well-being then meaning in life. Nonetheless, same serial multiple mediation model is not observed from other two materialistic dimensions including materialistic centrality and materialistic success.Conclusions: Materialistic happiness threatens people’s sense of meaning in life. This relationship can be explained under the framework of Self-Determination Theory. Issues such as how different types of happiness facilitate the constitution of meaning in life, and new investigation directions on materialism and meaning in life research have been discussed.
New Magellanic penguin Spheniscus magellanicus colony in a subantarctic island: good or bad news?
Authors: Rey Andrea Raya , Balza Ulises , Domato Ignacio , Zunino Francisco 
Published: July 2022

Abstract
This paper provides data on a newly discovered colony for Magellanic penguin Spheniscus magellanicus in the southwest Atlantic Ocean. A new settlement for the species in the area was found while conducting southern rockhopper penguin Eudyptes chrysocome studies in the San Juan de Salvamento colony in Staten Island, Argentina. The newly established colony holds 88 breeding pairs. While the origin of the founders was not recognized, it is possible that individuals recently reached from the nearby Observatorio Island colony located ~ 20 km away. New colonies grant populations to expand and colonize new areas which holds promise in a climate change scenario. Nonetheless, this expansion might be in detriment of sibling species that already occupied the area.
Co-occurrence of health risk behaviors and associated factors among adolescent students: Findings from a cross-sectional study in Bangladesh
Authors: Rahman Md. Estiar 
Published: July 2022

Abstract
Aim: This study aimed to determine the prevalence and associated factors of co-occurring health risk behaviors among Bangladeshi adolescents.Subject and Methods: A cross-sectional study involving 637 adolescent students in three high schools and three colleges in Dhaka city, Bangladesh, was conducted from November 2019-February 2020. Participants were selected using a multistage sampling technique. A self-administered questionnaire was used to collect data and statistical package for social sciences, version 22.0 was used to perform data analysis.Results: The participants comprised of 57.1% male, and their mean age was 15.65 ± 1.58 years. Among the participants, 2.4 % (n=15) reported one health risk behavior, while 0.3 % (n = 2) reported no risk behaviors. The prevalence of co-occurring health risk behaviors among the participants was 26.4%. Logistic regression analysis showed that co-occurrence of health risk behaviors was associated with participants aged with 17-18 years (OR = 3.11; 95% CI = 1.95-4.95, p < .001), college students (OR = 2.74, 95% CI = 1.90-3.94, p < .001), participants having secondary level of fathers’ education (OR=2.63; 95% CI=1.68-4.09, p < .001), participants having secondary level of mothers’ education (OR = 1.84; 95% CI = 1.12-3.02, p = .015), living apart form family (OR = 2.22; 95% CI = 1.37-3.59, p = .001), poor academic performance (OR=2.06; 95% CI = 1.26-3.36, p = .004), and reporting truancy (OR = 1.81; 95% CI = 1.20-2.72, p = .005).Conclusion: The findings emphasize the need for effective interventions to prevent risk behavior practices among adolescents.