bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical and molecular aspects of human pegiviruses in the interaction host and infectious agent
Authors: Samadi Mehdi , Salimi Vahid , Haghshenas Mohammad Reza , Miri Seyed Mohammad , Mohebbi Seyed Reza , Ghaemi Amir 
Published: March 2022

Abstract
Background
Human pegivirus 1 (HPgV-1) is a Positive-sense single-stranded RNA (+ ssRNA) virus, discovered in 1995 as a Flaviviridae member, and the closest human virus linked to HCV. In comparison to HCV, HPgV-1 seems to be lymphotropic and connected to the viral group that infects T and B lymphocytes. HPgV-1 infection is not persuasively correlated to any known human disease; nevertheless, multiple studies have reported a connection between chronic HPgV-1 infection and improved survival in HPgV-1/HIV co-infected patients with a delayed and favorable impact on HIV infection development. While the process has not been thoroughly clarified, different mechanisms for these observations have been proposed. HPgV-1 is categorized into seven genotypes and various subtypes. Infection with HPgV-1 is relatively common globally. It can be transferred parenterally, sexually, and through vertical ways, and thereby its co-infection with HIV and HCV is common. In most cases, the clearance of HPgV-1 from the body can be achieved by developing E2 antibodies after infection.

Main body
In this review, we thoroughly discuss the current knowledge and recent advances in understanding distinct epidemiological, molecular, and clinical aspects of HPgV-1.

Conclusion
Due to the unique characteristics of the HPgV-1, so advanced research on HPgV-1, particularly in light of HIV co-infection and other diseases, should be conducted to explore the essential mechanisms of HIV clearance and other viruses and thereby suggest novel strategies for viral therapy in the future.
Knockdown of CD44 inhibits proliferation, migration and invasion of osteosarcoma cells accompanied by downregulation of cathepsin S
Authors: Kong Lingwei , Ji Hairu , Gan Xintian , Cao Sheng , Li Zhehong , Jin Yu 
Published: March 2022

Abstract
Background
Osteosarcoma (OS) is a malignant bone tumour of mesenchymal origin. These tumours are characterised by rich vascularisation, therefore promoting rapid proliferation and facilitating metastasis. CD44 has been reported to be involved in OS, but its role and molecular mechanisms in the pathogenesis of the disease are not fully determined.

Methods
In this study, we investigated the antitumor effect of CD44 on the development of OS and further explored the molecular mechanisms. The expression of CD44, cathepsin S and MMP-9 was detected by Western blot (WB) and reverse transcription-polymerase chain reaction (RT-qPCR) in different cell lines (MG63, U2OS OS and hFOB 1.19). To elucidate the role of CD44 in OS, MG63 and U2OS cells were treated with small interference RNA (siRNA) to knock down CD44, and the knockdown efficiency was validated with GFP and RT-qPCR. Furthermore, cell proliferation was assayed using Cell Counting Kit‑8 (CCK-8) and colony formation assays, and cell migration and invasion were assayed by transwell and wound-healing assays.

Results
We found that CD44 expression in the MG63 and U2OS OS cell lines was markedly increased compared to that of the human osteoblast hFOB 1.19 cell line. Knockdown of CD44 inhibited proliferation, migration and invasion of MG63 and U2OS cells. Cathepsin S expression in the MG63 and U2OS OS cell lines was increased compared to that of the human osteoblast hFOB 1.19 cell line. When CD44 was knocked down, its expression level went down.

Conclusion
Taken together, our data reinforced the evidence that CD44 knockdown inhibited cell proliferation, migration and invasion of OS cells accompanied by altered expression of cathepsin S. These findings offer new clues for OS development and progression, suggesting CD44 as a potential therapeutic target for OS.
HIV infection is not associated with perioperative blood loss in patients undergoing total hip arthroplasty
Authors: Wei Qifeng , Cai Gaorui , Chen Guoneng , Shen Maoye , Gao Ya , Ning Xianjia , Wang Jinghua , Jiang Wenxue 
Published: March 2022

Abstract
Background
Patients with HIV have a higher prevalence of thrombocytopenia than those without HIV infection, increasing their risk of substantial perioperative blood loss (PBL) during total hip arthroplasty (THA). This study aimed to evaluate PBL risk factors in HIV-infected patients undergoing THA.

Methods
Eighteen HIV+ patients (21 hip joints) and 33 HIV− patients (36 joints) undergoing THA were enrolled in this study. PBL was calculated using the Gross equation, which comprises total blood loss (TBL), dominant blood loss (DBL), and hidden blood loss (HBL). Risk factors for post-THA PBL in both patient populations was evaluated using multivariable linear regression.

Results
At baseline, the HIV+ patients were younger, more likely to be male and to have elevated hemoglobin and albumin levels, and lower erythrocyte sedimentation rates than HIV− patients. There were no differences in the T-lymphocyte subsets or coagulation function between the two groups. Age and albumin level were identified as potential HBL risk factors after THA, and albumin level was associated with higher TBL. The unadjusted linear regression analysis showed that the HBL and TBL were significantly higher in HIV+ patients than in HIV− patients. However, after adjusting for other factors, no differences in DBL, HBL, or TBL were observed between HIV− and HIV+ patients.

Conclusion
PBL was similar in both groups undergoing THA, regardless of their HIV-infection status. THA surgery is a safe and effective procedure in HIV+ patients.
Transfemoral aortic valve replacement in a nonagenarian with aortic stenosis and cardiac amyloidosis: case report
Authors: Akras Deena , Bullinger Keith , Kondapaneni Meera , Siraj Aisha , Akhrass Rami , Aneja Ashish 
Published: March 2022

Abstract
Background
Cardiac amyloidosis (CA) is diagnosed with increasing frequency in the elderly population with severe aortic stenosis (AS), especially with the low-flow, low- gradient phenotype. Prognosis is poor with no treatment.

Case presentation
The patient is a 94-year-old active male who presented with a stroke that fully resolved. He was found to have low-flow, low-gradient severe AS, along with concomitant CA. Gradients across the aortic valve worsened with the dobutamine challenge test. He underwent successful transfemoral aortic valve replacement (TAVR) and did well postoperatively, where he remained in the hospital for only one day. Treatment of his CA with Tafamidis was recommended; however, the patient declined due to its cost and personal preference.

Conclusion
To our knowledge, we report on one of the oldest patients to undergo TAVR for low-flow, low-gradient AS with concurrent CA (AS-CA). It might be prudent to screen elderly patients with AS for CA, as prognosis is worse with medical management alone. TAVR has overall improved survival in patients with AS-CA and is considered the procedure of choice, as these patients are typically older and at higher risk for surgical intervention.
Discomfort of postoperative patients with aortic dissection after discharge: telephone follow-up analysis of a cross-sectional study
Authors: Lang Xiaorong , Huang Sufang , Wang Quan , Feng Danni , Xiao Yaru , Li Miqi , Guo Zhiran , Zhou Quan 
Published: March 2022

Abstract
Background
Postoperative discomfort is one of the important manifestations of disease changes, but few studies have reported detailed description of postoperative discomfort in patients with aortic dissection after discharge. The aim of this study is to investigate the discomfort symptoms and to explore the possible influencing factors of discomfort symptoms.

Method
This cross-sectional study based on convenience sampling collected medical records from 999 patients hospitalized in Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of science and technology, Wuhan, Hubei, China from January 1, 2019 to December 31, 2019. Postoperative patients with first onset and confirmed aortic dissection were eligible for follow-up. Telephone follow-up was conducted from July 20, 2020 to August 20, 2020. Symptoms of discomfort were reported by patients or their immediate family members. Univariate and multivariable logistic regression analysis were performed to identify factors associated with symptoms of discomfort.

Results
A total of 675 patients were followed up, 185 patients (27.4%) were lost to follow-up, and the remaining 490 patients were divided into survival group (N = 428) and death group (N = 62) and were included in the study. There was no difference in gender and age among the three groups. 152 of 428 patients reported discomfort. The uncomfortable symptoms of postoperative patients were diverse, and mainly manifested as back and chest pain (32.24%, 49/152), chest tightness (15.79%, 24/152), dizzy (10.53%, 16/152) and weakness (10.53%, 16/152). Multivariable logistic regression analysis of postoperative discomfort showed length of discharge (OR 0.995; P 0.018; 95% CI 0.990–0.999) and positive history of drinking (OR 3.519; P 0.018; 95% CI 1.236–10.022) were significant among patients with Stanford A AD, and diagnosis was made in the first visiting hospital (OR 0.395; P 0.001; 95% CI 0.230–0.677) was a protective factor for patients.

Conclusions
The incidence of postoperative discomfort in patients with aortic dissection was high and the symptoms were diverse and not single. In order to reduce the possibility of postoperative discomfort, it is important to formulate effective public policies to limit the public to drink alcohol and timely diagnose aortic dissection. Long term follow-up is necessary for patients with aortic dissection to observe the recovery process of aortic dissection.
Cinnamomi Ramulus inhibits the growth of colon cancer cells via Akt/ERK signaling pathways
Authors: Pan Boyu , Xia Yafei , Gao Zilu , Zhao Gang , Wang Liangjiao , Fang Senbiao , Liu Liren , Yan Shu 
Published: March 2022

Abstract
Background
Colon cancer (CC) ranks the second highest mortality rate among malignant tumors worldwide, and the current mainstream treatment regimens are not very effective. The unique efficacy of Chinese herb medicine (CHM) for cancer has recently attracted increasing attention. Cinnamomi Ramulus (CR), as a classic CHM, has been widely used in the treatment of a variety of diseases for hundreds of years in China, but its specific pharmacological mechanism against CC needs to be fully evaluated.

Methods
TCMSP and China National Knowledge Infrastructure database were utilized to predict the candidate ingredients of CR, and TCMSP and SwissTargetPrediction database were also employed to predict the drug targets of the candidate ingredients from CR. We subsequently evaluated the therapeutic effect of CR by orally administrating it on CC-bearing mice. Next, we further identified the potential CC-related targets by using Gene Expression Omnibus (GEO) database. Based on these obtained targets, the drug/disease-target PPI networks were constructed using Bisogenet plugin of Cytoscape. The potential core therapeutic targets were then identified through topological analysis using CytoNCA plugin. GO and KEGG enrichment analyses were performed to predict the underlying mechanism of CR against CC. Furthermore, these in silico analysis results were validated by a series of cellular functional and molecular biological assays. UPLC–MS/MS method and molecular docking analysis were employed to identify the potential key components from CR.

Results
In this study, we firstly found that CR has potential therapeutic effect on cancer. Then, oral administration of CR could inhibit the growth of CC cells in C57BL/6 mice, while inhibiting the viability and motility of CC cells in vitro. We obtained 111 putative core therapeutic targets of CR. Subsequent enrichment analysis on these targets showed that CR could induce apoptosis and cell cycle arrest in CC cells by blocking Akt/ERK signaling pathways, which was further experimentally verified. We identified 5 key components from the crude extract of CR, among which taxifolin was found most likely to be the key active component against CC.

Conclusions
Our results show that CR as well as its active component taxifolin holds great potential in treatment of CC.
Laparoscopic versus open resections in the posterosuperior liver segments within an enhanced recovery programme (ORANGE Segments): study protocol for a multicentre randomised controlled trial
Authors: Kuemmerli Christoph , Fichtinger Robert S. , Moekotte Alma , Aldrighetti Luca A. , Aroori Somaiah , Besselink Marc G. H. , D’Hondt Mathieu , Díaz-Nieto Rafael , Edwin Bjørn , Efanov Mikhail , Ettorre Giuseppe M. , Menon Krishna V. , Sheen Aali J. , Soonawalla Zahir , Sutcliffe Robert , Troisi Roberto I. , White Steven A. , Brandts Lloyd , van Breukelen Gerard J. P. , Sijberden Jasper , Pugh Siân A. , Eminton Zina , Primrose John N. , van Dam Ronald , Hilal Mohammed Abu ,   on behalf of the ORANGE trials collaborative
Published: March 2022

Abstract
Background
A shift towards parenchymal-sparing liver resections in open and laparoscopic surgery emerged in the last few years. Laparoscopic liver resection is technically feasible and safe, and consensus guidelines acknowledge the laparoscopic approach in the posterosuperior segments. Lesions situated in these segments are considered the most challenging for the laparoscopic approach. The aim of this trial is to compare the postoperative time to functional recovery, complications, oncological safety, quality of life, survival and costs after laparoscopic versus open parenchymal-sparing liver resections in the posterosuperior liver segments within an enhanced recovery setting.

Methods
The ORANGE Segments trial is an international multicentre randomised controlled superiority trial conducted in centres experienced in laparoscopic liver resection. Eligible patients for minor resections in the posterosuperior segments will be randomised in a 1:1 ratio to undergo laparoscopic or open resections in an enhanced recovery setting. Patients and ward personnel are blinded to the treatment allocation until postoperative day 4 using a large abdominal dressing.
The primary endpoint is time to functional recovery. Secondary endpoints include intraoperative outcomes, length of stay, resection margin, postoperative complications, 90-day mortality, time to adjuvant chemotherapy initiation, quality of life and overall survival.
Laparoscopic liver surgery of the posterosuperior segments is hypothesised to reduce time to functional recovery by 2 days in comparison with open surgery. With a power of 80% and alpha of 0.04 to adjust for interim analysis halfway the trial, a total of 250 patients are required to be randomised.

Discussion
The ORANGE Segments trial is the first multicentre international randomised controlled study to compare short- and long-term surgical and oncological outcomes of laparoscopic and open resections in the posterosuperior segments within an enhanced recovery programme.

Trial registration
ClinicalTrials.gov NCT03270917. Registered on September 1, 2017. Before start of inclusion. Protocol version: version 12, May 9, 2017
Do biometric payment systems work during the COVID-19 pandemic? Insights from the Spanish users' viewpoint
Authors: Liébana-Cabanillas Francisco , Muñoz-Leiva Francisco , Molinillo Sebastián , Higueras-Castillo Elena 
Published: March 2022

AbstractTechnological developments are changing how users pay for goods and services. In the context of the COVID-19 (coronavirus disease 2019) pandemic, new payment systems have been established to reduce contact between buyer and seller. In addition to the pandemic, the future is payment processing is also uncertain due to the new EU security regulations of the Payment Services Directive (PSD2). Biometric payments one option that would guarantee the security of transactions and reduce the risk of contagion. This research analyses the intention to recommend the use of the mobile phone as a tool for collecting payments in a shop using iris reading as a biometric measure of the buyer. The moderating effect of the fear of contagion in the proposed relationships was also analysed. An online survey was carried out, which yielded a sample of 368 respondents. The results indicate that the main antecedents of intention to use, which precedes intention to recommend, are perceived trust, habit, personal innovativeness and comfort of use. Additionally, the moderating effect of COVID-19 was checked among users with a higher perception of risk. The results obtained have interesting implications for purchase management among manufacturers and retailers.
The governance of imported 2019-nCov infections: What can be learned from China’s experience?
Authors: Li Hao , He Jiaxin , Chen Jiayu , Pan Shuning , Feng Jiehan , Liu Shuang 
Published: March 2022

AbstractDelta and Omicron variants of 2019-nCoV are still spreading globally, and many imported infections have been identified in China as well. In order to control the spread chain from imported to local, China has implemented the dynamic Covid-zero policy. In this article we summarized China’s governance models and practices of fighting potential imported infections in two directions. One targets at international travelers, which can be outlined as four lines of defense: customs epidemic prevention, quarantine upon arrival, relevant laws and regulations, and community tracking. The other is against other vectors potentially carrying 2019-nCoV, which can be outlined by three lines of defense: customs epidemic prevention, disinfection and personal protection, and information management. However, there are still some challenges that are yet to be addressed, such as illegal immigration, accidental occupational exposure to 2019-nCoV, etc. China’s experience indicates that no country can stay safe during the global pandemic as long as there are local outbreaks in other countries, and active prevention and control measures based on science and a complete set of laws and regulations are still necessary at current stage. What’s more, accountable government commitment and leadership, strengthened health and social governance systems, and whole society participation are required. It is suggested that the global community continue to closely cooperate together and take active rather than passive actions to block the potential imported 2019-nCoV from causing local spreading.
Optimal Location and Sizing of Conglomerate DG- FACTS using an Artificial Neural Network and Heuristic Probability Distribution Methodology for Modern Power System Operations
Authors: Siddiqui Anwar Shahzad , Prashant  
Published: March 2022

AbstractIn existing power system networks, the positioning and sizing of multi-DG is critical at the optimum locations for effective energy management. Initially optimal power flow is assessed using the NR method (without DG) in which performance parameters such as real power loss, accuracy, selectivity and MSE are obtained, but in an undesirable manner. To meet load demand; multi-DGs are placed and their optimal locations are assessed by the proposed heuristic probability distribution methodology and an ANN because existing techniques provides poor performance parameters for selecting the location and sizing of DGs. The optimal positions of multi-DGs are estimated in terms of performance parameters including real power loss of transmission network, accuracy, selectivity and MSE, while the performance parameters obtained with the ANN are better than the heuristic pdf. Then, the sizing of multi-DGs is evaluated in relation to active and reactive power. It is found that that sizes of multi-DGs are smaller with ANN than with heuristic pdf. It is preferable to connect the buses having lowest real power losses with the smallest multi-DGs. The performance analysis is tested in the standard IEEE 9- bus and IEEE 57- bus systems on Simulink. To improve the distortion level in real and reactive power, multi-FACTS namely TCSC, TSC and STATCOM are used. The switching of TCSC and TSC is done by SPWM while STATCOM switching is controlled with ANFIS. The locations of multi-FACTS devices are chosen for buses having larger distortion and the sizing of multi-FACTS devices is also optimally decided. The application of multi-FACTS devices helps to improve power quality and fulfill load demand with minimal size in order to make the system economical.
Local seismic culture in Iranian vernacular architecture: evidence from Yazd earthen architecture
Authors: Azizi-Bondarabadi Hamed , Haji Sadeghi Neda 
Published: March 2022

AbstractIran, located in one of the most earthquake-prone regions of the world, has experienced a very large number of destructive earthquakes that brought about considerable loss of human life and economic consequences. The country possesses a vast number of monumental and vernacular built heritages that are constantly threatened by earthquakes. As vernacular techniques for dwellings comply with the needs, comfort, utility, and functionality of people, it is expected that endemic earthquake-resistant techniques make an essential contribution to Iranian vernacular architecture. The city of Yazd presents a unique and rich vernacular for earthen construction within Iranian architectural heritage, which has resulted in the recognition of the historic fabric of the city as a World Heritage Site since 2017. Considering the importance of exploring different local seismic cultures worldwide, this paper introduces an essential part of Iranian local seismic culture by recognising and classifying the vernacular earthquake-resistant techniques used in the earthen architecture of the city of Yazd. The techniques were collected by surveying the historic fabric, using data available in the literature, and interviewing traditional builders and local conservators. Next, the seismic influence of each technique was evaluated using examples from the performance of adobe constructions during the 2003 Bam earthquake.
Generating a labeled data set to train machine learning algorithms for lithologic classification of drill cuttings
Authors: Becerra Daniela , Pires de Lima Rafael , Galvis-Portilla Henry , Clarkson Christopher R. 
Published: March 2022

Despite significant developments in the past few years in the application of machine learning algorithms for the lithologic classification of rock samples, publicly available labeled data sets are very scarce. We open source a fully labeled data set containing more than 16,000 scanning electron microscopy (SEM) images of drill cutting samples—mounted on thin sections—from a low-permeability reservoir in western Canada. We develop a simplified image processing workflow to segment and isolate the rock chips into individual SEM images, which in turn are used to identify, classify, and quantify rock types based on textural characteristics. In addition, using this data set, we explore the use of convolutional neural networks (CNNs) as a baseline tool for acceleration and automatization of rock-type classification. Without significant modifications to popular CNN models, we obtain an accuracy of approximately 90% for the test set. Results demonstrate the potential of CNN as a fast approach for lithologic classification in low-permeability siltstone reservoirs. In addition to making the data set publicly available, we believe our workflow to segment and isolate drill cutting samples in individual images of rock chips will facilitate future research of drill cuttings properties (e.g., lithology, porosity, and particle size) using machine learning algorithms.