bluuebunny/crossref_metadata_2025_split huggingface dataset:
In silico optimization of RNA-protein interactions for CRISPR-Cas13-based antimicrobials
Authors: Park Ho-min , Park Yunseol , Berani Urta , Bang Eunkyu , Vankerschaver Joris , Van Messem Arnout , De Neve Wesley , Shim Hyunjin 
Published: June 2022

AbstractRNA-protein interactions are crucial for diverse biological processes. In prokaryotes, RNA-protein interactions enable adaptive immunity through CRISPR-Cas systems. These defense systems utilize CRISPR RNA (crRNA) templates acquired from past infections to destroy foreign genetic elements through crRNA-mediated nuclease activities of Cas proteins. Thanks to the programmability and specificity of CRISPR-Cas systems, CRISPR-based antimicrobials have the potential to be repurposed as new types of antibiotics. Unlike traditional antibiotics, these CRISPR-based antimicrobials can be designed to target specific bacteria and minimize detrimental effects on the human microbiome during antibacterial therapy. Here, we explore the potential of CRISPR-based antimicrobials by optimizing the RNA-protein interactions of crRNAs and Cas13 proteins. CRISPR-Cas13 systems are unique as they degrade specific foreign RNAs using the crRNA template, which leads to non-specific RNase activities and cell cycle arrest. We show that a high proportion of the Cas13 systems have no colocalized CRISPR arrays, and the lack of direct association between crRNAs and Cas proteins may result in suboptimal RNA-protein interactions in the current tools. Here, we investigate the RNA-protein interactions of the Cas13-based systems by curating the validation dataset of Cas13 protein and CRISPR repeat pairs that are experimentally validated to interact, and the candidate dataset of CRISPR repeats that reside on the same genome as the currently known Cas13 proteins. To find optimal CRISPR-Cas13 interactions, we first validate the 3-D structure prediction of crRNAs based on their experimental structures. Next, we test a number of RNA-protein interaction programs to optimize the in silico docking of crRNAs with the Cas13 proteins. From this optimized pipeline, we find a number of candidate crRNAs that have comparable or better in silico docking with the Cas13 proteins of the current tools. This study fully automatizes the in silico optimization of RNA-protein interactions as an efficient preliminary step for designing effective CRISPR-Cas13-based antimicrobials.
Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET
Authors: Dutka Przemysław , Metskas Lauren Ann , Hurt Robert C. , Salahshoor Hossein , Wang Ting-Yu , Malounda Dina , Lu George , Chou Tsui-Fen , Shapiro Mikhail G. , Jensen Grant J. 
Published: June 2022

SUMMARYGas vesicles (GVs) are gas-filled protein nanostructures employed by several species of bacteria and archaea as flotation devices to enable access to optimal light and nutrients. The unique physical properties of GVs have led to their use as genetically-encodable contrast agents for ultrasound and MRI. Currently, however, the structure and assembly mechanism of GVs remain unknown. Here we employ cryo-electron tomography to reveal how the GV shell is formed by a helical filament of highly conserved GvpA subunits. This filament changes polarity at the center of the GV cylinder—a site that may act as an elongation center. High-resolution subtomogram averaging reveals a corrugated pattern of the shell arising from polymerization of GvpA into a β-sheet. The accessory protein GvpC forms a helical cage around the GvpA shell, providing structural reinforcement. Together, our results help explain the remarkable mechanical properties of GVs and their ability to adopt different diameters and shapes.
Digital innovation through networking among agro-food SMEs: the role of R&D projects
Authors: Silvestri Raffaele , Ingrao Carlo , Fiore Mariantonietta , Carloni Elisa 
Published: June 2022

PurposeDigitalization is the great global challenge of the twenty-first century including technologies, like, Internet-of-Things, Big Data and block-chain. New digital innovation can play a crucial role for the competitiveness and sustainability of agro-food small and medium-size enterprises (SMEs) but in Europe it has been adopted by only 25% of farmers. Joined participation in EU R&D projects can boost SME's digital innovation. The paper aims to analyze how business networking, within a R&D project, affects the digital innovation of agro-food SMEs.Design/methodology/approachThe paper investigates a single longitudinal case study focused on the local development phase of the EU Horizon 2020 project “Eugenius”, which aims to develop a European business network able to provide Earth observation services for natural resources management in agriculture. The focus is on the dyadic relationship between two firms, the winery Tormaresca and the digital innovation provider Planetek.FindingsResults highlight that the process of commercialization of Planetek's innovative services significantly passes through a multifaceted process of development and adaptation of technology to the specific needs of the user company, allowed by the research project with no expense for both companies during the experimentation phase.Originality/valueThe paper deals with a new underexplored topic thus giving interesting insights into how the R&D project participation affects agro-food SMEs in adopting digital innovations, which are difficult to acquire directly, and how this influences the value creation process and their organizational structure.
The new stage and mission of researching the history of socialist political economy with Chinese characteristics
Authors: Li Jiaxiang 
Published: June 2022

PurposeThe aim of this paper is reviewing the discipline development course of the history of socialist political economy with Chinese characteristics and recognising the changes of its development and its historic mission in the new stage will be beneficial to the construction of socialist political economy with Chinese characteristics from the perspective of doctrinal history.Design/methodology/approachIn this paper from the aspect of discipline formation and development, the history of China’s socialist political economy has experienced two stages: emergence and formation (the first stage) and steady development (the second stage). It has explored new research fields and improved the quality of research levels. However, the role of studying the history of socialist political economy with Chinese characteristics has not been fully played regarding satisfying the needs of constructing socialist political economy with Chinese characteristics.FindingsIn this study when the construction of socialist political economy with Chinese characteristics entered a new era, the study of the history of socialist political economy also entered a new stage, showing new features in terms of research objectives, principles, scale and methods.Originality/valueTherefore, the research on the history of socialist political economy with Chinese characteristics should be highly emphasised, and the focus on serving the construction of socialist political economy with Chinese characteristics should be its historic mission and core task. Also, researchers should pay attention to changing ideas, laying a good foundation, highlighting key points, building platforms and broadening horizons.
Work-to-family conflict, family satisfaction and engagement nexus: insights from millennial managers
Authors: Nwachukwu Chijioke , Chládková Helena , Moses Chinonye Love , Vu Hieu Minh 
Published: June 2022

PurposeMillennials’ perception of work, personal and family life is different from other generations. Building on studies on workforce generations, this paper aims to uncover not only the effect of work-to-family conflict (WFC) and family satisfaction (FS) on millennial managers engagement but also the moderating effect of (gender and marital status) in predicting engagement.Design/methodology/approachThis paper used a survey to collect data from managers of selected companies in Nigeria. In total, 127 questionnaires were analysed using the partial least square structural equation modelling method.FindingsResults reveal that the relationship between WFC, FS and millennial managers’ engagement is direct and significant. Besides, the effect of WFC on engagement is stronger in men and for married. The effect of FS on engagement is greater for women than men and for married.Practical implicationsThis study informs the research community as well as practitioners and affirms the importance of supportive work-to-family life and FS in fostering millennial managers’ engagement with their organisations.Originality/valueThis study is among the initial attempts to evaluate the impact of WFC and FS on engagement among millennial managers, especially in Africa, Nigeria in particular. The results identify millennial managers’ unique perspectives towards engagement and how gender and marital status may enhance engagement. With millennials fast attaining leadership roles, such knowledge is important.
The transformation from manual to smart warehousing: an exploratory study with Swedish retailers
Authors: Kembro Joakim , Norrman Andreas 
Published: June 2022

PurposeTo meet customers' expectations on shorter lead times, high product availability, flexibility, and variation in delivery and return options, retailers have turned their attention to warehousing and are making big investments in technology. Currently, technology providers are pushing for smart warehousing, a new and under-researched phenomenon. This study aims to conceptualize the term and examine pathways toward implementing smart warehousing.Design/methodology/approachAn exploratory survey was administered to 50 leading Swedish retailers in varying segments. A two-tailed t-test for equality of means was used to detect significant differences between current and future states.FindingsThe study found that future smart warehouses will be automated, autonomous, digital, and connected, but that retailers will follow different paths along this journey, driven by contextual trends, e.g. sales growth, wider product assortment, shorter lead-time offerings, and integration of brick-and-mortar and online stores. Interestingly, the study revealed that many of the retailers that aim to create smart warehouses in five years are not the retailers with the most developed technology today.Research limitations/implicationsThe paper operationalizes smart warehousing in two dimensions: degree of automation and degree of digitalization and connectivity of information platforms. Based on the findings, 16 theoretical propositions are put forth that, based on contextual factors, explain different pathways for retailers to implement smart warehousing.Practical implicationsThe empirical insights and theoretical discussions provide practically useful guidance, including outlined trends, for selecting and benchmarking automation and complementary technologies in warehouse operations.Originality/valueThis paper conceptualizes and operationalizes smart warehousing – an original approach. It is also one of the first to investigate the technological transformation in retail warehousing empirically, explaining how and why retailers choose different pathways toward smart warehousing.
Paved with good intentions: role of situational and dispositional influences on employees’ unethical pro-organizational behavior
Authors: Chhabra Bindu , Srivastava Shalini 
Published: June 2022

Purpose
The rise in the instances of ethical scandals in recent times has brought to light the hitherto ignored phenomenon of unethical proorganizational behavior (UPB) by employees. Drawing upon the social exchange theory, this study aims to examine the role of perceived organizational support (POS) and organizational identification on UPB. The research also investigated the moderating role of core self-evaluation (CSE) and positive reciprocity beliefs.

Design/methodology/approach
The hypotheses were tested with three-wave survey data collected from 306 executives from India. Data was analyzed using mediation and moderated mediation analyses on PROCESS v 3.0 macro.

Findings
Results showed that organizational identification mediated the relationship between POS and UPB. Further, CSE and positive reciprocity beliefs were seen to moderate the mediated relationship providing support for the moderated mediation framework.

Practical implications
The study highlights the importance of clear code of ethics, communication of ethical guidelines and ethical behavior of the managers as some of the ways to reduce the menace of UPB. Further, managers should be more vigilant toward the employees low on CSE and high on positive reciprocity beliefs as they are more prone to engage in UPB given their identification with the organization.

Originality/value
To the best of the authors’ knowledge, this is the first study to investigate the mediating role of organizational identification in the relationship between POS and UPB. Further, to the best of the authors’ knowledge, no previous study has explored the moderating role of CSE and positive reciprocity beliefs in the aforesaid mediation.
Consumer information technology use in the post-pandemic workplace: a post-acceptance adaptation perspective
Authors: Yin Pengzhen , Wang Chuang , Liang Liang 
Published: June 2022

PurposePersonally owned mobile information and communication technologies (MICT) have been widely and routinely used for work purposes in the post-pandemic workplace. Drawing on adaptive structuration theory for individuals (ASTI), this study investigates the antecedents (i.e. characteristics of MICT) and outcomes (i.e. employee affective and cognitive well-being) of routine MICT use in the remote work context.Design/methodology/approachThe research model was empirically tested via a survey of 430 working professionals who use personal MICT for work purposes in the remote work context.FindingsResults show that the routine MICT use increases employee affective well-being (i.e. job satisfaction) and mitigates cognitive well-being (i.e. technology overload). The mediation effects of routine MICT use on the relationships between its characteristics (autonomy and timeliness) and employee well-being (i.e. job satisfaction and technology overload) were also found.Originality/valueExisting research on remote work has widely focused on employee productivity and performance, while attention has rarely been paid to the effects of the technology-driven “new normal” on employee well-being. Grounded in ASTI, this study identifies three MICT characteristics as sources of user adaptive structuration, which impact employees' routine MICT use behavior and further influence employee affective and cognitive well-being. This research can help understand employees' personal MICT use adaptive behavior and improve their well-being.
School middle leaders as instructional leaders: building the knowledge base of instruction-oriented middle leadership
Authors: Tang Jianjing , Bryant Darren A. , Walker Allan David 
Published: June 2022

PurposeThis paper aims to explore developments over the past 25 years in the knowledge base on instruction-oriented middle leadership in schools. The authors document the trends in the literature since middle leadership began to attract scholarly interest in the late 1990s and explore the shifting structural and content patterns in the knowledge base.Design/methodology/approachThe authors use a topographic methodology to analyse both structural elements and major results drawing from 147 peer-reviewed journal articles.FindingsThe authors draw on the review's outcomes to propose a model that frames a core set of middle leaders' instructional leadership practices. They also identify the personal, departmental, organizational and external influences shaping middle leadership practices and identify a lack of research conducted outside Anglo–American societies. This gap in the literature suggests the need for the increased study of middle leadership in different national settings and systems and how these influence the practice and enactment of middle leaders around instruction. There is also a need to employ a greater range of methodologies to understand middle leaders' instructional roles.Research limitations/implicationsThe paper lays a foundation for the future development of middle leadership for instruction and highlights signposts to guide future inquiry.Originality/valueThe paper contributes to the middle leader knowledge base by focussing on their enactment of instructional leadership.
The intersectional effect of age and gender on the work–life balance of managers
Authors: Thrasher Gregory R. , Wynne Kevin , Baltes Boris , Bramble Reed 
Published: June 2022

PurposeAlthough there is a small body of empirical research on the working lives of managers, both the popular media and the academic literature tend to ignore the distinct ways that role identities such as age and gender intersect to create a complex work–life interface for diverse managers. This gap is especially surprising considering that managerial roles are defined by unique demands and expectations that likely intersect with the differential life course shifts experienced by men and women, which has the potential to create specific challenges across the work and life domains of managers. The current study aims to address this gap through an intersectional examination of the non-linear effects of age and gender on the work–life balance of managers.Design/methodology/approachUsing a sample of 421 managers, the authors apply statistical tests of the incremental validity of non-linear interaction terms to examine the complex relationship between age, gender and work–life balance.FindingsResults support a non-linear U-shaped main effect of age on leader work–life balance. This effect is moderated by gender, however, with a non-linear U-shaped effect of age on work–life balance being supported for male managers – with female managers displaying no effect of age on work–life balance.Practical implicationsBased on these findings, the authors highlight the need for increased availability of flexible schedules and employee empowerment for managers as well as general employees.Originality/valueThe current study offers one of the first tests of the intersection of age and gender on the work–family interface of managers.
Programmatic advertising in online retailing: consumer perceptions and future avenues
Authors: Ciuchita Robert , Gummerus Johanna Katariina , Holmlund Maria , Linhart Eva Larissa 
Published: June 2022

PurposeDigital advertising enables retailers to rely on large volumes of data on consumers and even leverage artificial intelligence (AI) to target consumers online with personalised and context-aware advertisements. One recent example of such advertisements is programmatic advertising (PA), which is facilitated by automatic bidding systems. Given that retailers are expected to increase their use of PA in the future, further insights on the pros and cons of PA are required. This paper aims to enhance the understanding of the implications of PA use for retailers.Design/methodology/approachA theoretical overview is conducted that compares PA to traditional advertising, with an empirical investigation into consumer attitudes towards PA (an online survey of 189 consumers using an experimental design) and a research agenda.FindingsConsumer attitudes towards PA are positively related to attitudes towards the retailer. Further, perceived ad relevance is positively related to attitudes towards PA, which is moderated by (1) consumer perceptions of risks related to sharing their data with retailers online and (2) consumer perceptions of AI's positive potential. Surprisingly, the disclosed use of AI for PA does not significantly influence consumer attitudes towards PA.Originality/valueThis paper contributes to the literature on technology-enabled services by empirically demonstrating that ad relevance drives consumer attitudes towards PA. This paper further examines two contingencies: risk beliefs related to data (i.e. the source of PA) and perceptions of AI (i.e. the somewhat nebulous technology associated with PA) as beneficial. A research agenda illuminates central topics to guide future research on PA in retailing.
The impact of perceived organizational politics and political risk on project success through ethical leadership and the psychological contract
Authors: Turi Jamshid Ali , Sarfraz Muddassar 
Published: June 2022

PurposePolitical risk devastates social and economic developmental projects. Countries with political stability attract foreign direct investment (FDI) and engage domestic investment corporations. This study aims to investigate the impact of perceived organizational politics and political risk on project success, considering the moderating and mediating roles of ethical leadership and the psychological contract.Design/methodology/approachA multimethod approach was adopted in this work that includes an exploratory content analysis to confirm the latent factors of the variables under study. A measurement scale was developed and tested for perceived organizational politics, political risk, the psychological contract and ethical leadership in the projectized environment. Lastly, cross-sectional data were collected from the senior-level professionals of the projectized organizations and analyzed using SPSS and SMARTPLS techniques.FindingsThe findings indicate that ethical leadership and the psychological contract mitigate political risk. The study recommends that developing countries emphasize well-defined policies and standard operating procedures to streamline the project design and execution processes.Research limitations/implicationsThe study claims that ethical leaders can play a vital role in mitigating perceived organizational politics and political risk and maximizing project value through the psychological contract.Originality/valueAlthough previous research predicts that ethical leadership has very little effect on project success, this study provides critical theoretical and practical contributions to research on project success regarding leadership expertise and the psychological contract.
How do FDI inflows curvilinearly affect carbon emissions? Threshold effects of energy service availability and cleanliness
Authors: Chen Fuzhong , Jiang Guohai , Dong Kangyin 
Published: June 2022

AbstractTo examine the relationship between foreign direct investment (FDI) inflows and carbon emissions from the perspective of the threshold effects of energy service availability and energy consumption cleanliness, this study employs the panel data of 16 Regional Comprehensive Economic Partnership member countries for the period 1990–2019. Using panel threshold models, this study finds that energy service availability and energy consumption cleanliness are two significant threshold variables in the process by which FDI inflows affect carbon emissions. Before they reach a certain level, FDI inflows aggravate carbon emissions, but this association becomes negative after reaching the threshold level. Furthermore, this study also indicates that the threshold values are different depending on national conditions. Specifically, countries with favourable technological development or stronger environmental regulations have lower threshold values for the two variables, while countries with a higher degree of industrial added value have higher threshold values. Based on these findings, this study recommends enhancing energy service availability and energy consumption cleanliness and formulating related industrial, technological and legal policies based on different national conditions.