bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differentiated HIV services for transgender people in four South African districts: population characteristics and HIV care cascade
Authors: Bothma Rutendo , O'Connor Cara , Nkusi Jolie , Shiba Vusi , Segale Jacob , Matsebula Luyanda , Lawrence J. Joseph , van der Merwe L. Leigh‐Ann , Chersich Matthew , Hill Naomi 
Published: October 2022

AbstractIntroductionTransgender people in South Africa are disproportionately affected by HIV, discrimination and stigma. Access to healthcare and health outcomes are poor. Although integrating gender‐affirming healthcare with differentiated HIV prevention, care and treatment services has shown improvement in HIV service uptake and health outcomes among transgender people, evidence is lacking on the implementation of differentiated service delivery models in southern Africa. This article describes a differentiated service delivery model across four South African sites and transgender individuals who access these services. We assess whether hormone therapy (HT) is associated with continued use of pre‐exposure prophylaxis (PrEP) and viral load suppression.MethodsIn 2019, differentiated healthcare centres for transgender individuals opened in four South African districts, providing gender‐affirming healthcare and HIV services at a primary healthcare level. Routine programme data were collected between October 2019 and June 2021. Descriptive statistics summarized patient characteristics and engagement with HIV prevention and treatment services. We conducted a multivariate logistic regression analysis to determine whether HT was associated with viral load suppression and PrEP continued use.ResultsIn the review period, we reached 5636 transgender individuals through peer outreach services; 86% (4829/5636) of them accepted an HIV test and 62% (3535/5636) were linked to clinical services. Among these, 89% (3130/3535) were transgender women, 5% (192/3535) were transgender men and 6% (213/3535) were gender non‐conforming individuals. Of those who received an HIV test, 14% (687/4829) tested positive and 91% of those initiated antiretroviral treatment. Viral load suppression was 75% in this cohort. PrEP was accepted by 28% (1165/4142) of those who tested negative. Five percent (161/3535) reported ever receiving HT through the public healthcare system. Service users who received HT were three‐fold more likely to achieve viral load suppression. We did not find any association between HT and continued use of PrEP.ConclusionsA differentiated HIV and gender‐affirming service delivery model at a primary healthcare level is feasible and can enhance service access in South Africa. HT can improve HIV clinical outcomes for transgender people. As trust is established between the providers and population, uptake of HIV testing and related services may increase further.
An evaluation of nine culturally tailored interventions designed to enhance engagement in HIV care among transgender women of colour in the United States
Authors: Rebchook Gregory M. , Chakravarty Deepalika , Xavier Jessica M. , Keatley JoAnne G. , Maiorana Andres , Sevelius Jae , Shade Starley B. ,   the SPNS Transgender Women of Color Study Group
Published: October 2022

AbstractIntroductionTransgender women (TW) worldwide have a high prevalence of HIV, and TW with HIV encounter numerous healthcare barriers. It is critical to develop evidence‐informed interventions to improve their engagement in healthcare to achieve durable viral suppression (VS). We evaluated whether participation in one of nine interventions designed specifically for TW was associated with improved engagement in HIV care among transgender women of colour (TWC).MethodsBetween 2013 and 2017, nine US organizations implemented nine distinct and innovative HIV care engagement interventions with diverse strategies, including: individual and group sessions, case management and navigation, outreach, drop‐in spaces, peer support and/or incentives to engage TWC with HIV in care. The organizations enrolled 858 TWC, conducted surveys, captured intervention exposure data and extracted medical record data. Our evaluation of the interventions employed a pre‐post design and examined four outcomes—any HIV care visit, antiretroviral therapy (ART) prescription, retention in HIV care and VS (both overall and among those with a clinic visit and viral load test), at baseline and every 6 months for 24 months. We employed logistic generalized estimating equations to assess the relative odds of each outcome at 12 and 24 months compared to baseline.ResultsOverall, 79% of participants were exposed to at least one intervention activity. Over 24 months of follow‐up, participants received services for a median of over 6 hours (range: 3–69 hours/participant). Compared to baseline, significantly (p<0.05) greater odds were demonstrated at both 12 and 24 months for three outcomes: prescription of ART (ORs: 1.42 at 12 months, 1.49 at 24 months), VS among all participants (ORs: 1.49, 1.54) and VS among those with a clinic visit and viral load test (ORs: 1.53, 1.98). The outcomes of any HIV care visit and retention in HIV care had significantly greater odds (ORs: 1.38 and 1.58, respectively) only at 12 months compared to baseline.ConclusionsThese evaluation results illustrate promising approaches to improve engagement in HIV care and VS among TWC with HIV. Continued development, adaptation and scale‐up of culturally tailored HIV care interventions for this key population are necessary to meet the UNAIDS 95‐95‐95 goals.
Reaching transgender populations in Zambia for HIV prevention and linkage to treatment using community‐based service delivery
Authors: Mwango Linah , Toeque Mona‐Gekanju , Lindsay Brianna , Tembo Kalima , Sakala Henry , Reggee Sean , Malunga Sibusiso Mimi , Kabwe McLean , Kafunda Ina , Olufunso Adebayo , Mwila Annie , Okuku Jackson , Kancheya Nzali , Nkwemu Kennedy , Mumba Daliso , Hachaambwa Lottie , Sheneberger Robb , Blanco Natalia , Lavoie Marie‐Claude , Stafford Kristen A. , Claassen Cassidy W. 
Published: October 2022

AbstractIntroductionTransgender and gender‐diverse communities in Zambia are highly vulnerable and experience healthcare differently than cisgender persons. The University of Maryland, Baltimore (UMB) supports projects in Zambia to improve HIV case‐finding, linkage and antiretroviral treatment (ART) for Zambia's transgender community. We describe programme strategies and outcomes for HIV prevention, testing and ART linkage among transgender communities.MethodsUMB utilizes a differentiated service delivery model whereby community health workers (CHWs) recruited from key populations (KPs) reach community members through a peer‐to‐peer approach, with the support of local transgender civil society organizations (CSOs) and community gatekeepers. Peer CHWs are trained and certified as HIV testers and psychosocial counsellors to offer counselling with HIV testing and prevention services in identified safe spaces. HIV‐negative people at risk of HIV infection are offered pre‐exposure prophylaxis (PrEP), while those who test positive for HIV are linked to ART services. CHWs collect data using the standardized facility and community tools and a dedicated DHIS2 database system. We conducted a descriptive analysis examining HIV testing and prevention outcomes using proportions and comparisons by time period and geographic strata.ResultsFrom October 2020 to June 2021, across Eastern, Lusaka, Western and Southern Provinces, 1860 transgender persons were reached with HIV prevention messages and services. Of these, 424 (23%) were tested for HIV and 78 (18%) tested positive. Of the 346 HIV‐negative persons, 268 (78%) eligible transgender individuals were initiated on PrEP. ART linkage was 97%, with 76 out of the 78 transgender individuals living with HIV initiating treatment. Programme strategies that supported testing and linkage included peer CHWs, social network strategy testing, same‐day ART initiation and local KP CSO support. Challenges included non‐transgender‐friendly environments, stigma and discrimination, the high transiency of the transgender community and the non‐availability of transgender‐specific health services, such as hormonal therapy.ConclusionsPeer KP CHWs were able to reach many members of the transgender community, providing safe HIV testing, PrEP services and linkage to care. Focusing on community gatekeepers and CSOs to disburse health messages and employ welcoming strategies supported high linkage to both PrEP and ART for transgender people in Zambia.
HIV awareness and prevention strategies among transgender women in the Eastern and Southern United States: findings from the LITE Study
Authors: Aguayo‐Romero Rodrigo A. , Cannon Christopher M. , Wirtz Andrea L. , Cooney Erin E. , Mayer Kenneth H. , Reisner Sari L. ,   the American Cohort to Study HIV Acquisition among Transgender Women (LITE) Study Group
Published: October 2022

AbstractIntroductionTransgender women (TW) experience an increased risk of human immunodeficiency virus (HIV) acquisition. This study identified patterns of HIV awareness and prevention strategies used by TW who were not living with HIV.MethodsData were drawn from a baseline survey of the LITE Study, a multi‐site cohort of TW in Eastern and Southern United States (March 2018–August 2020). We conducted a latent class analysis to identify classes of HIV awareness and prevention strategies among TW who reported past 12‐month sexual activity (N = 958) using 10 variables spanning HIV knowledge, receipt and use of HIV prevention strategies, and sexual practices. Due to differences across the cohort arms, classes were estimated separately for TW enrolled in site‐based versus online study arms. We identified demographic characteristics, gender‐affirming indicators and HIV vulnerabilities associated with class membership.ResultsFour parallel classes emerged: class 1 “limited strategies—less sexually active” (15% and 9%, site‐based and online, respectively), class 2 “limited strategies—insertive sex” (16%/36%), class 3 “limited strategies—receptive sex” (33%/37%) and class 4 “multiple strategies—insertive and receptive sex” (36%/18%). Across all classes, condomless sex, pre‐exposure prophylaxis (PrEP)/post‐exposure prophylaxis (PEP) prevention knowledge and awareness were high but reported PrEP/PEP use was low. Compared with class 1, membership in class 4 was associated with being a person of colour (site‐based OR = 2.15, 95% CI = 1.15–4.00, online OR = 4.54, 95% CI = 1.09–18.81) increased odds of self‐perceived medium‐to‐high HIV risk (site‐based OR = 4.12, 95% CI = 2.17–7.80, online OR = 11.73, 95% CI = 2.98–46.13), sexually transmitted infections (STI) diagnosis (site‐based OR = 6.69, 95% CI = 3.42–13.10, online OR = 8.46, 95% CI = 1.71–41.78), current sex work (site‐based OR = 6.49, 95% CI = 2.61–16.11, online OR = 10.25, 95% CI = 1.16–90.60) and 2–4 sexual partners in the last 3 months (site‐based OR = 2.61, 95% CI = 1.33–5.13). Class 3, compared with class 1, had increased odds of current sex work partners (site‐based OR = 3.09, 95% CI = 1.19–8.07) and of having 2–4 sexual partners in the last 3 months (site‐based OR = 3.69, 95% CI = 1.85–7.39).ConclusionsTW have varied HIV awareness and prevention strategy utilization, with clear gaps in the uptake of prevention strategies. Algorithms derived from latent class membership may be used to tailor HIV prevention interventions for different subgroups and those reached through facility‐based or digital methods.
Metabolomics analysis of antiquitin deficiency in cultured human cells and plasma: Relevance to pyridoxine‐dependent epilepsy
Authors: Crowther Lisa M. , Poms Martin , Zandl‐Lang Martina , Abela Lucia , Hartmann Hans , Seiler Michelle , Mathis Déborah , Plecko Barbara 
Published: October 2022

AbstractDeficiency of antiquitin (α‐aminoadipic semialdehyde dehydrogenase), an enzyme involved in lysine degradation and encoded by ALDH7A1, is the major cause of vitamin B6‐dependent epilepsy (PDE‐ALDH7A1). Despite seizure control with high dose pyridoxine (PN), developmental delay still occurs in approximately 70% of patients. We aimed to investigate metabolic perturbations due to possible previously unidentified roles of antiquitin, which may contribute to developmental delay, as well as metabolic effects of high dose pyridoxine supplementation reflecting the high doses used for seizure control in patients with PDE‐ALDH7A1. Untargeted metabolomics by high resolution mass spectrometry (HRMS) was used to analyze plasma of patients with PDE‐ALDH7A1 and two independently generated lines of cultured ReNcell CX human neuronal progenitor cells (NPCs) with CRISPR/Cas mediated antiquitin deficiency. Accumulation of lysine pathway metabolites in antiquitin‐deficient NPCs and western‐blot analysis confirmed knockdown of ALDH7A1. Metabolomics analysis of antiquitin‐deficient NPCs in conditions of lysine restriction and PN supplementation identified changes in metabolites related to the transmethylation and transsulfuration pathways and osmolytes, indicating a possible unrecognized role of antiquitin outside the lysine degradation pathway. Analysis of plasma samples of PN treated patients with PDE‐ALDH7A1 and antiquitin‐deficient NPCs cultured in conditions comparable to the patient plasma samples demonstrated perturbation of metabolites of the gamma‐glutamyl cycle, suggesting potential oxidative stress‐related effects in PN‐treated patients with PDE‐ALDH7A1. We postulate that a model of human NPCs with CRISPR/Cas mediated antiquitin deficiency is well suited to characterize previously unreported roles of antiquitin, relevant to this most prevalent form of pyridoxine‐dependent epilepsy.
The association between human papillomavirus and bladder cancer: Evidence from meta‐analysis and two‐sample mendelian randomization
Authors: Sun Jian‐Xuan , Xu Jin‐Zhou , Liu Chen‐Qian , An Ye , Xu Meng‐Yao , Zhong Xing‐Yu , Zeng Na , Ma Si‐Yang , He Hao‐Dong , Hu Jia , Liu Zheng , Wang Shao‐Gang , Xia Qi‐Dong 
Published: October 2022

AbstractIntroductionBladder cancer (BCa) is the 10th most common type of cancer worldwide, and human papillomavirus (HPV) is the most common sexually transmitted infection. However, the relationship between HPV infection and the risk of BCa is still controversial and inconclusive.MethodsThis systematic review and meta‐analysis were conducted following the PRISMA 2020 reporting guideline. This study searched four bibliographic databases with no language limitation. The databases included PubMed (Medline), EMBASE, Cochrane Library, and Web of Science. Studies evaluating the interaction between HPV infection and the risk of BCa from inception through May 21, 2022, were identified and used in this study. This study estimated the overall and type‐specific HPV prevalence and 95% confidence intervals (95% CI) using Random Effects models and Fixed Effects models. In addition, this study also calculated the pooled odds ratio and pooled risk ratio with 95% CI to assess the effect of HPV infection on the risk and prognosis of bladder cancer. Two‐sample mendelian randomization (MR) study using genetic variants associated with HPV E7 protein as instrumental variables were also conducted.ResultsThis study retrieved 80 articles from the four bibliographic databases. Of the total, 27 were case–control studies, and 53 were cross‐sectional studies. The results showed that the prevalence of HPV was 16% (95% CI: 11%–21%) among the BCa patients, most of which were HPV‐16 (5.99% [95% CI: 3.03%–9.69%]) and HPV‐18 (3.68% [95% CI: 1.72%–6.16%]) subtypes. However, the study found that the prevalence varied by region, detection method, BCa histological type, and sample source. A significantly increased risk of BCa was shown for the positivity of overall HPV (odds ratio [OR], 3.35 [95% CI: 1.75–6.43]), which was also influenced by study region, detection method, histological type, and sample source. In addition, the study found that HPV infection was significantly associated with the progression of BCa (RR, 1.73 [95% CI: 1.39–2.15]). The two‐sample MR analysis found that both HPV 16 and 18 E7 protein exposure increased the risk of BCa (HPV 16 E7 protein: IVW OR per unit increase in protein level = 1.0004 [95% CI: 1.0002–1.0006]; p = 0.0011; HPV 18 E7 protein: IVW OR per unit increase in protein level = 1.0003 [95% CI: 1.0001–1.0005]; p = 0.0089).ConclusionIn conclusion, HPV may play a role in bladder carcinogenesis and contribute to a worse prognosis for patients with BCa. Therefore, it is necessary for people, especially men, to get vaccinated for HPV vaccination to prevent bladder cancer.
Time dependency in the radiofrequency lesion formation for a local impedance guided catheter in an ex vivo experimental model
Authors: Kawano Daisuke , Mori Hitoshi , Tsutsui Kenta , Fukaya Hidehira , Tanaka Naomichi , Narita Masataka , Sasaki Wataru , Matsumoto Kazuhisa , Ikeda Yoshifumi , Arai Takahide , Nakano Shintaro , Kato Ritsushi 
Published: October 2022

AbstractBackgroundThe local impedance (LI) is an emerging technology that monitors tissue‐catheter coupling during radiofrequency (RF) ablation. The relationships between the LI, RF delivery time, and lesion formation remain unclear.MethodsUsing an LI‐enabled RF catheter in an ex vivo experimental model, RF lesions were created combined with various steps in the power (40 and 50 W), CF (10 g, 30 g, and 50 g), and time (10s, 20s, 30s, 40s, 50s, and 60s at 40 W and 5 s, 10s, 20s, 30s, 40s, 50s, and 60s at 50 W). The correlations between the LI drop, lesion size, and RF delivery time were evaluated. The rate of change in the time‐dependent gain in the LI, depth, and diameter and the time to reach 90% decay of the peak dY/dT (time to 90% decay) were assessed.ResultsThe correlation between the LI drop and ablation time revealed non‐linear changes. The time to a 90% decay in the LI drop differed depending on the RF ablation setting and was always shorter with the 50 W setting than 40 W setting. The LI drop always correlated with the lesion formation under all ablation power settings. Deeper or wider lesions were predominantly created within the time to 90% decay of the LI drop.ConclusionThe LI drop was useful for predicting lesion sizes. Deeper or wider lesions cannot be obtained with a longer ablation than the 90% decay time of the LI drop. A shorter ablation than the 90% decay time of the LI drop would be preferable for an effective ablation.
Objective determination of the winter monsoon onset dates and its interannual variability in Malaysia
Authors: Chenoli Sheeba Nettukandy , Diong Jeong Yik , Mohd Nor Mohd Fadzil Firdzaus , Yip Weng Sang , Xavier Prince , Chang Nursalleh K. , Abu Samah Azizan 
Published: October 2022

AbstractThe northeast/winter monsoon over Malaysia often leads to extreme rainfall events and floods over the windward side of terrain due to the strong northeasterly winds. Comparatively, much less rain falls over the other are sheltered by the mountain ranges. Based on this consideration, the onset of monsoon in the region is best determined operationally using 925 hPa winds. Therefore, we propose the first, simple, single‐variable‐based method to determine the onset dates of winter monsoon is yet reliable and can be closely monitored for operational purposes. The onset date is defined in the third pentad when the average of three pentads of 925 hPa northerly wind speed is greater than 1 m·s−1, and at least one of three pentads must be greater than 2.5 m·s−1. The study also investigates the relationship between the interannual variability of onset date and El Niño–Southern Oscillation (ENSO) and Indian Ocean Dipole (IOD). Late‐onset can be expected when La Niña develops in the boreal fall season. However, a similar association between monsoon onset and El Niño condition was not observed. The lead–lag correlation between the IOD and the onset dates shows that the onset is significantly negatively correlated with the IOD. During negative (positive) IOD events, a delay (early) onset is expected. Results also show that the relationship between the onset and IOD is more robust and linear than the ENSO.
SARS‐CoV‐2 severity classification from plasma sample using confocal Raman spectroscopy
Authors: Banerjee Arghya , Halder Ankit , Jadhav Priyanka , Sarkar Anushka , Hole Arti , Shastri Jayanthi S. , Agrawal Sachee , Chilakapati Murali Krishna , Srivastava Sanjeeva 
Published: October 2022

AbstractThe world is on the brink of facing coronavirus's (COVID‐19) fourth wave as the mutant forms of viruses are escaping neutralizing antibodies in spite of being vaccinated. As we have already witnessed that it has encumbered our health system, with hospitals swamped with infected patients observed during the viral outbreak. Rapid triage of patients infected with SARS‐CoV‐2 is required during hospitalization to prioritize and provide the best point of care. Traditional diagnostics techniques such as RT‐PCR and clinical parameters such as symptoms, comorbidities, sex and age are not enough to identify the severity of patients. Here, we investigated the potential of confocal Raman microspectroscopy as a powerful tool to generate an expeditious blood‐based test for the classification of COVID‐19 disease severity using 65 patients plasma samples from cohorts infected with SARS‐CoV‐2. We designed an easy manageable blood test where we used a small volume (8 μl) of inactivated whole plasma samples from infected patients without any extra solvent usage in plasma processing. Raman spectra of plasma samples were acquired and multivariate exploratory analysis PC‐LDA (principal component based linear discriminant analysis) was used to build a model, which segregated the severe from the non‐severe COVID‐19 group with a sensitivity of 83.87%, specificity of 70.60% and classification efficiency of 76.92%. Among the bands expressed in both the cohorts, the study led to the identification of Raman fingerprint regions corresponding to lipids (1661, 1742), proteins amide I and amide III (1555, 1247), proteins (Phe) (1006, 1034), and nucleic acids (760) to be differentially expressed in severe COVID‐19 patient's samples. In summary, the current study exhibits the potential of confocal Raman to generate simple, rapid, and less expensive blood tests to triage the severity of patients infected with SARS‐CoV‐2.
Dephytinized flaxseed flours by phytase enzyme and fermentation: functional ingredients to enhance the nutritional quality of noodles
Authors: Yaver Elif 
Published: October 2022

AbstractBACKGROUNDPhytic acid is an antinutritional compound that reduces the bioavailability of minerals and proteins. Flaxseed is a rich source of unsaturated fatty acids and antioxidants. In this study, flaxseed flour was dephytinized by phytase enzyme and fermentation. Untreated and dephytinized flaxseed flours were used in noodle production at 0%, 10%, 20% and 30% levels to improve nutritional quality.RESULTSThe addition of flaxseed flours did not show an adverse effect on the cooking loss values of noodles (4.22–4.85%). Firmness values of noodles containing 10% flaxseed flour were close to the control (0% flaxseed flour). The ash, protein and fat levels of noodles were enhanced with the addition of flaxseed flours. The use of 30% phytase‐treated flaxseed flour in noodles elicited lower phytic acid concentration (1.35 g kg−1) than the control (1.52 g kg−1). The greatest total phenolic contents in noodles were obtained with the inclusion of fermented flour. Besides that, noodles made from flaxseed flours exhibited stronger antioxidant activity than the control. The overall acceptability scores of noodles containing 10% and 20% dephytinized flours were close to the control.CONCLUSIONThe findings demonstrated that the inclusion of dephytinized flaxseed flours up to 20% can allow the production of functional noodles with acceptable quality. Moreover, phytase enzyme treatment method revealed lower phytic acid content than fermentation method in noodles and better antioxidant properties than untreated noodles. © 2022 Society of Chemical Industry.