bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of synaptic connectivity in schizophrenia by mutual neuron-microglia interaction
Authors: Breitmeyer Ricarda , Vogel Sabrina , Heider Johanna , Wüst Richard , Keller Anna-Lena , Binner Anna , Fitzgerald Julia , Fallgatter Andreas , Volkmer Hansjurgen 
Published: July 2022

Abstract
The examination of post-mortem brain tissue suggests synaptic loss as a central pathological hallmark of schizophrenia. Synaptic loss has been shown to be related to increased inflammation in the central nervous system. Aberrant activation of microglia, the immune cells of the human brain, may account for neuronal damage in schizophrenia. Induced pluripotent stem cells represent a promising tool for studying neuropsychiatric disease mechanisms. A recent challenge has been the development of protocols to derive microglia as well as neurons from schizophrenia patient-derived stem cells to better understand the mechanistic contribution of neuroinflammation to the disease. To address this, we present a co-culture model of neurons and microglia, both of human origin to show increased susceptibility of neurons to microglia-like cells derived from schizophrenia patients. Analysis of IBA-1 expression, NFκB signaling, transcription of inflammasome-related genes, and caspase-1 activation shows that enhanced, intrinsic inflammasome activation in patient-derived microglia exacerbates neuronal deficits such as synaptic loss in schizophrenia. Anti-inflammatory pretreatment of microglia with minocycline specifically rescued aberrant synapse loss in schizophrenia and reduced microglial activation. These findings open up possibilities for further research in larger cohorts, focused clinical work and longitudinal studies that could facilitate earlier therapeutic intervention.
Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model
Authors: Satitsri Saravut , Akrimajirachoote Nattaphong , Nunta Kanokkan , Ruennarong Nitwarat , Amnucksoradej Orawan , Muanprasat Chatchai 
Published: July 2022

Abstract
Post-weaning diarrhea in piglets is a major problem resulting in significant loss in pig production. This study aimed to investigate effects of piperine, an alkaloid abundantly found in black peppers, on biological activities related to pathogenesis of post-weaning diarrheas using a porcine duodenal enteroid model, a newly established intestinal stem cell-derived in vitro model recapitulating physiology of porcine small intestinal epithelia. Porcine duodenal enteroid models were treated with disease-relevant pathological inducers with or without piperine (8 µg/mL and/or 20 µg/mL) before measurements of oxidative stress, mRNA expression of proinflammatory cytokines, nuclear factor-kappa B (NF-κB) nuclear translocation, barrier leakage, and fluid secretion. We found that piperine (20 µg/mL) inhibited H2O2-induced oxidative stress, TNF-α-induced mRNA expression of proinflammatory cytokines without affecting NF-κB nuclear translocation, and prevented TNF-α-induced barrier leakage in porcine duodenal enteroid monolayers. Importantly, piperine inhibited fluid secretion induced by both forskolin and heat-stable toxins (STa) in a three-dimensional model of porcine duodenal enteroids. Collectively, piperine possesses both anti-inflammatory and anti-secretory effects in porcine enteroid models. Further research and development of piperine may provide novel interventions for treatment of post-weaning porcine diarrheas.
Leading on co-production programmes in five UK collaborative research partnerships (2008-2018): responses to four tensions from system leaders using auto-ethnography
Authors: Graaf Peter van der , Kislov Roman , Smith Helen , Langley Joe , Hamer Natalie , Cheetham Mandy , Wolstenholme Daniel , Cooke Jo , Mawson Sue 
Published: July 2022

Abstract
BackgroundDespite a growing enthusiasm for co-production in health care services and research, research on co-production practices is lacking. An abundance of conceptual frameworks, guidelines and principles are available but little empirical research is conducted on ‘how to do’ co-production of research to improve health care services. This paper brings together leadership insights from collaborative research partnerships in the UK on co-production practices with the aim to inform practical guidance for new partnerships facilitating the co-production of applied health research in the future. MethodsUsing an auto-ethnographic approach, experiential evidence was elicited through collective sense making from recorded conversations between the research team and leaders of five UK collaborative research partnerships (CLAHRCS). This approach applies a cultural analysis and interpretation of the leads’ behaviours, thoughts and experiences of co-production between 2008 and 2018 in relation to the academics, health practitioners, policy makers, and third sector organisations involved in co-produced research projects within the collaborative research partnerships. ResultsThe findings highlight a variation of practices across CLAHRCs with the context in which co-production occurs largely determining the nature of the process and outcomes. We identified four tensions in doing co-production: 1) idealistic, tokenistic vs realistic narratives, 2) power differences and (lack of) reciprocity, 3) excluding vs including language and communication, 4) Individual motivation vs structural issues.ConclusionsThe tensions were productive in helping collaborative research partnerships tailor co-production practices to their local needs and existing opportunities. As a result, practices varied within and between partnerships, which should not be reduced to one gold standard for co-production but should be celebrated. The links between the tensions informed solutions in each context, with strategic leadership identified as an important starting point; however, this role needs to be developed into more distributed forms of leadership over time to facilitate co-production practices between partners. Facilitating structures for co-production enabled power sharing through capacity and capability building, which resulted in more inclusive language and communication, and more realistic practices of co-production in research. We provide recommendations for creating more meaningful narratives around co-production and facilitating power sharing between partners.
Evaluation of municipal landfills based on leachate transport and aquifer vulnerability by DRASTIC index
Authors: Moghaddam Hamid Kardan , Moghaddam Hossein Kardan , Mirzavand Mohammad , Yalali Amir hooman 
Published: July 2022

Abstract
One of the problems with the current environment due to population growth and development is the issue of Waste, landfill, and leachate production. Waste as a high-volume output source, multiple potential users for landfill sites, and leachate are problems. The study investigates the proper location of the municipal landfill using multi-criteria ANP and entropy. Accordingly, four main criteria were defined for analyzing two methods for an appropriate place in the GIS environment. These four main criteria were taken into account with 15 modeling sub-criteria. Finally, the entropy method identified five suitable locations using the ANP method, and four appropriate sites were determined. The distribution of the selected sites by the entropy and ANP methods showed that the ANP method is more distant than the entropy method, and the two sites, A4 and A5 positions are more distant than the other aquatic sites. To evaluate the identified sites, the vulnerability of groundwater resources downstream of identified sites and the rate of leachate movement from these sites were considered. Based on the results of the DRASTIC Vulnerability Index and MODPATH, a particle-tracking Model, it was found that the northeast part of the city has a longer affected area due to the higher slope and higher permeability than the selected locations in the northwest. Accordingly, the area affected by the A3 site is also the only place where leachate effects enter the aquifer within three years due to its proximity.
Identification of Laportea bulbifera using the Complete Chloroplast Genome as a Super-Barcode
Authors: wang Wenting , Wang Xingwen , Shi Yuhua , yin Qinggang , Gao Ranran , Wang Mengyue , Xiang Li , Wu Lan 
Published: July 2022

Abstract
Laportea bulbifera, a Miao medicine grown in karst areas, has exerts a unique curative effect on skin itching in the elderly, with an annual sales of > 100 million Yuan. Owing to the shortage of resources and large morphological variations in L. bulbifera, it is difficult to identify the species correctly using only traditional methods, which seriously affects the safety of drug usage for patients. This study obtained the complete high-quality L. bulbifera chloroplast (cp) genome, using second- and third-generation high-throughput sequencing. The cp genome was 149,911 bp in length, with a typical quadripartite structure. A total of 127 genes were annotated, including 83 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. There was an inverted small single copy (SSC) structure in the L. bulbifera cp genome, one large-scale rearrangement of ~ 39 kb excised in the SSC and IR regions. The complete cp genome sequence used as a super-barcode and the highly variable regions (ycf1, matK, and ndhD), especially matK, can be used as specific barcodes to accurately distinguish L. bulbifera from counterfeits and closely related species. This study is important for the identification of L. bulbifera and lays a theoretical foundation for elucidating the phylogenetic relationship of the species.
Characterizing bacterial pathogen causing sugar beet dry rot of root at harvest and its suppression by endophytic bacteria
Authors: Safara Somayeh , Harighi Behrouz , Amini Jahanshir , Bahramnejad Bahman 
Published: July 2022

Abstract
Several bacteria play essential roles in causing post-harvest sugar beet root rot. In 2018 and 2019, a survey in field and storage revealed the presence of sugar beet dry rot of root. Seventeen bacteria were isolated from the harvested roots from various geographic locations in West Azerbaijan, Iran. Pathogenicity test in sugar beet root slices revealed that isolate Buk12 showed dry rot similar to those observed in the storage after seven days. Based on the petiole test, Buk12 produced slight discoloration in leaf petiole that extended into the leaf. Molecular identification showed that strain Buk12 had a high similarity to Pseudomonas thivervalensis based on partial nucleotide sequencing of the 16S rRNA gene. Endophytic bacteria produce secondary metabolites (SMs) and volatile organic compounds (VOCs) that have a significant role in the control of phytopathogens. In this study, the antagonistic activity of endophytic bacteria Streptomyces sp. B86, Bacillus sp. S411, Pseudomonas sp. B451, Bacillus sp. Andi542, Streptomyces sp. Sh800, and Staphylococcus sp. Bt870 were screened and their VOCs effects on the virulence traits of Pseudomonas thivervalensis Buk12 were evaluated. All endophytic bacteria tested showed antagonistic activity against the bacterial pathogen in vitro. The results of enzymatic activity, HCN production, and siderophore production were varied amongst strains. The VOCs emitted from endophytic bacteria significantly reduced dry root symptoms and the growth of P. thivervalensis Buk12 at different levels. Our results revealed that VOCs produced by all endophytic bacteria significantly reduced swarming motility, chemotaxis behavior, and attachment of P. thivervalensis Buk12 cells to sugar beet roots. The VOCs produced by endophytic bacteria except for Streptomyces sp. B86 significantly reduced twitching motility by P. thivervalensis Buk12. The influence of VOCs on biofilm formation by bacterial pathogen was varied amongst endophytic bacteria. The gas chromatography-mass spectrometry (GC-MS) analysis revealed that strains B86, S411, B451, Andi542, Sh800, and Bt870 produced 13, 13, 15, 23, 16, and 24 VOCs, respectively with high quality. Our findings provide new information regarding the potential of VOCs with antibacterial activity and as a biocontrol tool against postharvest bacterial root rot. To the best of our knowledge, this is the first report about bacteria as sugar beet dry rot agent from Iran and the isolation of endophytic bacteria from sea beet plants with biocontrol activity.
Continuing cyclin-dependent kinase 4/6 inhibition in metastatic breast cancer patients previously treated with another cyclin-dependant kinase 4/6 inhibitor: a protocol for systematic review and meta-analysis
Authors: Aguilar Alicia de Luna , Fuentes Javier David Benitez , Arcilla Jorge Bartolome , Shah Richa , Avagyan Manushak , Frick Clara , Lorenzo Alfonso Lopez de Sa , Rojas Carmen Toledano , Anton Fernando Moreno , Saenz Jose Angel Garcia 
Published: July 2022

Abstract
Background
Breast cancer is the most frequent cancer in women worldwide. Most breast cancer cases are estrogen receptor positive and epidermal growth factor receptor 2 negative (ER+/HER2-). A small proportion of these cases are metastatic, with Cyclin-dependent kinase 4 and 6 (CDK4/6) inhibitors being the mainstay of treatment in this scenario. Evidence supports the existence of differences between the three approved cyclin-dependent kinase 4/6 inhibitors (CDK4/6i) in preclinical and clinical settings. However, literature regarding the use of a CDK4/6i in metastatic breast cancer patients (MBC) previously treated with another CDK4/6i is scarce.
Methods
A search will be performed on the PubMed, Embase, and Web of Science databases following Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines. In addition, a manual search of the San Antonio Breast Cancer Conference, American Society of Clinical Oncology annual conference, European Society of Medical Oncology annual conference, and European Society of Medical Oncology Breast annual conference will be performed.
Discussion
To our knowledge, no previous systematic review has evaluated the available evidence regarding the efficacy and safety of treatment with CDK4/6i in MBC previously treated with another CDK4/6i. This systematic review and meta-analysis will synthesise the existing data on the effectiveness and safety of using a CDK4/6i after prior exposure to another in MBC. It will also identify the gap in the literature for potential future research. This study's results will interest multiple audiences, including patients, their families, caregivers, and healthcare professionals. Results will be published in a peer-reviewed journal.
Systematic review registration:
PROSPERO CRD42022330355
Can global or national disability weights represent provincial level?
Authors: He Qin , Zhou Maigeng , Yin Peng , Jiang Bo , Qi Jinlei , Liu Yunning , Liu Jiangmei , You Jinling , Zhou Yuchang , Long Zheng , Xing Xiuya , Cheng Qianyao , Chen Yeji , Wang Huadong , Liu Zhirong 
Published: July 2022

Abstract
Background A universal set of disability weights(DWs) mainly based on the survey of North America, Australia and Europe, the participants in Asia was limited. The debate hasn’t yet settled whether a universal DW is desirable or useful. Taking the DWs of Anhui province in Central China as an introduction, through the same-cultural and cross-cultural comparisons, this paper attempts to answer the representative questions of DWs.Methods A web-based survey was conducted to estimate the DWs for 206 health states of Anhui province in 2020. Paired comparison (PC) data were analyzed and anchored by probit regression and fitting loess model. We compared the DWs in Anhui with other provinces in China and those in Global burden of disease (GBD) and Japan. Results Compared with Anhui province, the proportion of health states which showed 2 times or more differences ranged from 1.94% (Henan) to 11.17% (Sichuan) in China and domestic provinces. It was 19.88% in Japan and 21.51% in GBD 2103 respectively. In Asian countries or regions, most of the health states with top 15 DWs belonged to the category of mental, behavioral, and substance use disorders. But in GBD, most were infectious diseases and cancer. The differences of DWs in neighboring provinces were smaller than other geographically distant provinces or countries.Conclusion Global or national DWs may not represent provincial level, especially in the absence of the gold standards. And there is an urgent need for relevant gold standards.
Mothers' Decisions to Exclusively Breastfeed in Indonesia: The Implication of Socio-Economic and Mental Illness Factors
Authors: Relaksana Riki , Akbar Adhadian , Sihaloho Estro Dariatno , Ferdian Dani , Siregar Adiatma YM 
Published: July 2022

Abstract
Background:
In Indonesia, nearly half of all children aged < 6 months were not exclusively breastfed in 2017. This study aimed to estimate the cost of providing exclusive breastfeeding compared to partial exclusive breastfeeding and use of formula milk. This study also assessed the maternal socio-economic and mental health factors to providing exclusive breastfeeding.
Methods:
Data were collected in 2018 via a cross-sectional survey of 456 mothers in Bandung City and Purwakarta District who had children aged < 6 months. We used micro-costing to calculate the cost of productivity, equipment, supplies, and training for mothers when providing exclusive breastfeeding, partial exclusive breastfeeding, and formula milk. Logistic regression was used to determine the impact of several independent variables, including the mother's level of depression, on exclusive breastfeeding.
Results:
To breastfeed exclusively, a family needs to spend $ 116,95, which is less expensive than partial breastfeeding ($ 487,18) and providing only formula milk ($ 531,97). We also found that age, time spent on breastfeeding, and marital status positively influence the decision to breastfeed. On the other hand, working status, the number of children, and the total cost of the child’s nutrients negatively impact the decision. Finally, severe maternal depression symptoms were significantly negatively associated with the decision to exclusively breastfeed.
Conclusion:
The total cost of providing only formula milk is 3-times higher than the cost of exclusively breastfeeding, even after including the cost of providing breastfeeding time. The total cost and presence of severe depression symptoms are related to the mother’s decision to not exclusively breastfeed. This study found that exclusive breastfeeding is economically preferable to other methods and supports policies to reduce the time cost of exclusive breastfeeding and address the importance of the mother's mental health to ensure successful breastfeeding.
Diagnostic accuracy and significance of clinical analysis of liver stiffness measurement by Fibro touch in metabolic-related fatty liver disease
Authors: Qin Qian , Yan Hang , Chen Jingfeng , Wu Zhuoqing , Yang Yang , Su Yan , Huang Meng , Li Tiantian , Sun Yanfang , Wang Shoujun , Ding Suying 
Published: July 2022

Abstract
Background and Aim: We aimed to explore risk factors and construct a model of MAFLD with fibrosis fibrosis and compare the performance of the panels of APRI, FIB-4 and BARD.Methods: We retrieved clinical information and enrolled 3671 patients. Subjects were assigned to four groups: MAFLD, MAFLD with fibrosis, and MAFLD with advanced fibrosis. Multivariate regression analysis and randomforest model were to construct the model of MAFLD with fibrosis, the receiver operating characteristics (ROC) were used to compare the diagnostic efficacy of predictive model, APRI, BARD, FIB-4. Results: ①The proportion of fibrosis in MAFLD was higher than that of non-MAFLD group; Compared with the control group, advanced fibrosis (≥F3) has no statistical significance. ②Four variables were selected to build the model of MAFLD. The diagnostic accuracy (AUROC=0.730) was superior to that of APRI and random forest model. ③Multivariate logistic analysis showed that increased BMI and AST was risk factors for advanced fibrosis and elevated PLT was a protective factor for advanced fibrosis; The diagnostic power of the model (AUROC=0.714) was superior to that of the FIB-4 and BARD.Conclusion: The model of fibrosis stage with MAFLD is superior to other non-invasive markers of fibrosis of MAFLD, which can assist clinicians in screening and warning for potential risks of MAFLD with fibrosis.
Differences in soil microbial communities with successional stage depend on vegetation coverage and soil substrates in alpine desert shrublands
Authors: Dong Ruizhen , Wang Xiaoli , Wang Yanlong , Ma Yushou , Yang Shihai , Zhang Litian , Zhang Min , Qin Jinping , Quzha Renzeng 
Published: July 2022

Abstract
Background and aims In semiarid ecosystems, changes in plant communities are promoted under shrub canopies during restoration, but the link between shrub community restoration dynamics and changes in soil microbe communities is still unclear.Methods We characterized the community structure and plant interactions of soil microbes by combining different methodological approaches (including high-throughput sequencing of the 16S rRNA gene and ITS gene, analysis of phospholipid fatty acids (PLFAs) and chloroform fumigation) and the key driving factors along a successional gradient of Sophora moorcroftiana shrub community in the middle reaches of the Yarlung Zangbo River.Results Soil microbial biomass carbon (MBC) and nitrogen (MBN), total PLFAs, and alpha diversity increased significantly as the successional stage advanced, and MBC and MBN were positively correlated with the carbon and nitrogen contents in the soil. Mantel test showed that successional stage-induced changes in soil microbial beta diversity were mainly associated with shrub coverage and soil physicochemical properties. The relative abundances of bacterial PLFAs, particularly those of gram-negative bacteria, such as Bacteroidetes, Alphaproteobacteria and Betaproteobacteria, significantly decreased with succession; the opposite was true for Acidobacteria, Planctomycetes, Gemmatimonadetes Deltaproteobacteria and Gammaproteobacteria. However, the proportion of fungi did not significantly differ among the four successional stages; the dominant phyla were Ascomycota and Basidiomycota.Conclusion We suggest that shrubs directly shape soil microbial communities or indirectly affect such communities by altering soil substrates. Our findings advance the current understanding of sand-stabilizing plant–soil interactions during natural restoration and the reversal of desertification in stressful desert ecosystems.