bluuebunny/crossref_metadata_2025_split huggingface dataset:
The negative impact of opioids on cancer patients treated with immune checkpoint inhibitors: A systematic review and meta-analysis
Authors: Gao Ziming , Ju Mingguang , Liu Xiaofang , Zhou Heng , Wang Ruiying , Zheng Chen , Dong Daosong , Zhu Zhi , Li Kai 
Published: June 2022

Abstract
Background
As one of the most effective analgesics, opioids are essential for patients with cancer-related pain, even in the context of the opioid abuse crisis. The current meta-analysis aimed to identify whether concomitant exposure to opioids can affect the efficacy of ICIs and lead to a worse prognosis.
Methods
PubMed, Embase, and the Cochrane Library were searched Based on the PRISMA checklist, through April 2022, for the following terms: ("opioids" OR "concomitant medication") AND ("Neoplasm" OR "Carcinoma" OR "Cancer" OR "Tumor") AND ("Immunotherapy" OR "Immune Checkpoint Inhibitor" OR "PD-L1 Inhibitor" OR "PD-1 Inhibitor" OR "CTLA-4 Inhibitor"). The outcomes considered were overall survival (OS) and progression-free survival (PFS) calculated using the random-effects or fixed-effects model.
RESULTS
After screening 531 studies, a total of 7 articles involving 2690 patients were eligible for quantitative analysis. The use of opioids was negatively correlated with OS (HR = 1.75, 95%CI = 1.32–2.31, P < .001; I2 = 81%, P < .001) and significantly reduced the PFS (HR = 1.61, 95%CI = 1.41–1.83, P < .001; I2 = 0%, P = .63) of patients treated with ICIs. Similar results were obtained in each subgroup analysis. While NSAIDs could lead to poor OS (HR = 1.25, 95% CI = 1.03–1.51, P = 0.02) but not PFS (HR = 1.11, 95% CI = 0.89–1.39, P = 0.36) for ICIs patients. And sensitivity analyses confirmed the reliability of the results.
CONCLUSIONS
Opioids significantly reduced OS and PFS in patients receiving ICI therapy. Thus, the use of different types of opioids should be considered with caution, and it is necessary to actively develop alternative treatments.
Analysis of the top coal stability of the large section open-off cut under the gob in thick seams slicing mining
Authors: Chai Jing , Liu Yongliang , Gao Shigang , Yang Jianfeng , Zhang Dingding , Du Wengang , Ma Chenyang , Han Zhicheng 
Published: June 2022

Abstract
The reserved thickness of top coal has an important influence on the stability of a large section open-off cut under gob in the thick seams slicing mining. The destabilization extremum conditions of the open-off cut top coal were derived from by elastic-plastic theory, and the optical fiber sensing technology was utilized to monitor the top coal deformation law with different thicknesses (3, 3.5, and 4m) in the physical similar simulation experiment in the paper. The results show that the top coal thickness is greater than 3.4m without tension cracks. In the vertical direction, the top coal of the LSOOC is divided into mining and excavation disturbance zones under the influence of the upper slice coal mining and the excavation disturbance. In the direction of the span of the open-off cut, the top coal can be divided into the roof fall risk zone and the warning zone. The deformation changes from exponential to linear to logarithmic in the roof fall risk zone, and it changes from linear to logarithmic in the roof fall warning zone as the number of excavations increases. The sinking amount in the two zones is smaller as the thickness of the top coal becomes larger. It is comprehensively determined that the thickness of the top coal of open-off cut is set as 3.5m, the stability is moderate, and the field application shows that the integrity of the top coal is good after support, and the maximum off-layer value is 6mm, which can satisfy the production requirements.
Pharmacological Activities and Characterization of Phenolic and Flavonoid Compounds in Solenostemma argel Extract
Authors: Soliman Mohamed S. M. , Abdella Asmaa , Khidr Yehia A. , Osman Hassan G. O. , Aladadh Mohamed Abdullah , Elsanhoty Rafaat M. 
Published: June 2022

Abstract
Solenostemma argel is a desert medicinal plant indigenous to African countries. The goal of this work was to investigate the pharmacological properties of phenolic and flavonoid compounds present in the Solenostemma argel. Aerial parts (leaves and flowers) of Solenostemma argel were tested for antibacterial activity, antioxidant activity, cell viability and cytotoxic effects, and anti-inflammatory activity. Phenolic and flavonoid contents (HPLC), and volatile constituents (GC-MS) were also characterized. The antioxidant activity of Solenostemma argel extract increased from (34.47%) at a concentration of 10 µg/mL to (98.47%) at a concentration of 1280 µg/mL. The most sensitive organism was S. aureus with the highest inhibition zone and lowest MIC, with acetone extract. Whereas C. tropicalis was the most resistant, with the lowest inhibition zone. MTT assay indicated that the methanolic extract of Solenostemma argel had significant cytotoxic effects on the A549, Caco-2, and MDAMB-231 cell lines, respectively. The anti-inflammatory activity showed at lower concentrations of methanolic extract, and those effects were compared with indomethacin as a standard. Gallic was the most abundant phenolic acid, followed by syringic, p-coumaric, caffeic and ferulic acids, respectively. The Catechin and Quersestin, Luteolin and rutin flavonoids were also found in the methanolic extract. GC-mass analysis showed that aerial parts of Solenostemma argel were rich in 2-(5-methyl-5 vinyl tetrahydro-2-furanyl)-2-propanol (11.63%), hexanoic acid methyl ester (10.93%), 3-dioxolane,4-methyl-2-pentadecyl (9.69%), phenol, 2-(1,1-dimethylethyl) (8.50%). It can be concluded that methanolic extract of Solenostemma argel could be used as an available source of natural bioactive constituents with consequent health benefits such as antioxidants, anti-inflammation, anticancer and antimicrobial effects.
A Gibbs sampler for multi-species occupancy models
Authors: Clark Allan Ernest , Altwegg Res 
Published: June 2022

Abstract
Multi-species occupancy (MSO) models use detection-nondetection data from several species observed at different locations to estimate the probability that a particular species occupies a particular geographical region. The models are particularly useful for estimating the occupancy probabilities associated with rare species since they are seldom observed when undertaking field surveys. In this paper, we develop Gibbs sampling algorithms that can be used to fit various Bayesian MSO models to detection-nondetection data. Bayesian analysis of these models can be undertaken using statistical packages such as JAGS, Stan and NIMBLE , however, since these packages were not developed specifically to fit occupancy models, one often experiences long run-times when undertaking analysis. In a single season (single species) nonspatial and spatial occupancy modelling context, Clark and Altwegg (2019), show that special purpose Gibbs samplers can produce posterior chains that mix faster and have larger expected sampling rates (Holmes & Held, 2006) than those obtained using JAGS and Stan. These results suggest that such algorithms could potentially lead to significant reductions in the run-times of MSO models. This paper illustrates how to fit MSO models when the detection and occupancy processes are modelled using logistic link functions and apply these methods to a camera-trapping study undertaken by 1 Drouilly, Clark, and O’Riain (2018). Variable selection is undertaken using a reversible-jump Markov chain Monte Carlo (Barker & Link, 2013) algorithm. We found that the Gibbs sampling algorithm developed produces posterior samples that are identical to those obtained when using Stan, resulting in faster run-times and has a larger expected sampling rate than Stan when analysing the above-referenced data set.
A Head-to-Head comparison of fast-SENC and Feature Tracking to LV long axis strain for assessment of myocardial deformation in chest pain patients
Authors: Siry Deborah , Riffel Johannes , Salatzki Janek , André Florian , Weberling Lukas Damian , Ochs Marco , Atia Noura A. , Hillier Elizabeth , Albert David , Katus Hugo A. , Giannitsis Evangelos , Frey Norbert , Friedrich Matthias Gero 
Published: June 2022

Abstract
BackgroundMyocardial strain imaging has gained importance in cardiac magnetic resonance (CMR) imaging in recent years as an even more sensitive marker of early left ventricular dysfunction than left-ventricular ejection fraction (LVEF). fSENC (fast strain encoded imaging) and FT (Feature Tracking) both allow for reproducible assessment of myocardial strain. However, left-ventricular long axis strain (LVLAS) might enable an equally sensitive measurement of myocardial deformation as global longitudinal or circumferential strain in a more rapid and simple fashion. MethodsIn this study we compared the diagnostic performance of fSENC, FT and LVLAS for identification of cardiac illness (ACS, cardiac-non-ACS) in patients presenting with chest pain (initial hscTnT 5- 52 ng/l). Patients were prospectively recruited from the chest pain unit in Heidelberg. The CMR scan was performed within 1h after patient presentation. Analysis of LVLAS was compared to the GLS and GCS as measured by fSENC and FT. ResultsIn total 40 patients were recruited (ACS n=6, cardiac-non-ACS n=6, non-cardiac n=28). LVLAS was comparable to fSENC for differentiation between healthy myocardium and myocardial illness (GLS-fSENC AUC: 0.882; GCS-fSENC AUC: 0.899; LVLAS AUC: 0.771; GLS-FT AUC: 0.740; GCS-FT: 0.688). There was significant variability between the three techniques. Intra- and inter-observer variability (OV) was excellent for fSENC and FT, whilst for LVLAS the agreement was lower and levels of variability higher (intra-OV: Pearson >0.7, ICC>0.8; inter-OV: Pearson>0.65, ICC>0.8; CoV > 25%). ConclusionsAlthough there was significant bias between the three deformation parameters, reproducibility was excellent for both FT and fSENC. Whilst LVLAS demonstrated lower levels of agreement and higher variability, it was comparable to FT and fSENC for identification of myocardial illness and might be used as a rapid supporting parameter for assessment of left-ventricular function.
A Viroporin from SARS-CoV-2 Reduces the Infectivity of Human Immunodeficiency Virus type 1 (HIV-1)
Authors: Henke Wyatt , Waisner Hope , Arachchige Sachith Polpitiya , Kalamvoki Maria , Stephens Edward 
Published: June 2022

Abstract
Background: Viroporins are virally encoded ion channels that are involved in virus assembly and release. Human immunodeficiency virus type 1 (HIV-1) and influenza A virus encode for viroporins. The human coronavirus SARS-CoV-2 encodes for at least two viroporin proteins, a small 75 amino acid transmembrane protein known as the envelope (E) protein and a larger 370 amino acid protein known as Orf3a. Here, we compared the HIV-1 virus infectivity in the presence of four different β-coronavirus E proteins. Results: We observed that the SARS-CoV-2 and SARS-CoV E proteins reduced the release of infectious HIV-1 yields by approximately 100-fold while MERS-CoV or HCoV-OC43 E proteins restricted HIV-1 infectivity to a lesser extent. Mechanistically, we show that the E proteins reduced the levels of HIV-1 protein synthesis in cells and that that the E protein neither affected reverse transcription nor genome integration. However, SARS-CoV-2 E protein activated the ER-stress pathway associated with the phosphorylation of eIF-2α, which is known to attenuate protein synthesis in cells. Other ever-expressed proteins (HSV-1 gD, HIV-1 gp160 and EGFP) did not cause phosphorylation of eIF-2α. Finally, we show that unlike the Vpu protein of HIV-1 and the Spike protein of SARS-CoV-2, the four E proteins and the SARS-CoV-2 N protein did not significantly down-regulate bone marrow stromal cell antigen 2 (BST-2). Conclusions: The results of this study indicate that while viroporins from homologous viruses can enhance virus release, that we show a viroporin from a heterologous virus can suppress HIV-1 protein synthesis and virus release.
Diabetes during the COVID-19 pandemic: are people getting access to the right level of care?
Authors: Meyerowitz-Katz Gideon , Ferdousi Shahana , Maberly Glen , Astell-Burt Thomas 
Published: June 2022

Abstract
Introduction Avoidance of health services, in particular hospital and community services, is problematic for people with diabetes. Evidence has demonstrated that such missed attendances are associated with worse health, faster declines in functioning, and higher rates of mortality long-term. This paper investigated the impact of the pandemic on healthcare access across community and hospital care, including Virtual Care (VC) using several large datasets of GP and hospital services in western Sydney. Methods A retrospective cohort study using a time-series database of 173,805 HbA1c tests done at Blacktown and Mt Druitt hospitals and 1.8 million recorded consultations at GP clinics in the region was undertaken. Results The average rate of diabetes in ED fell from 17.8% pre-pandemic to 11% after January 2020 (p < 0.001). This rate varied substantially over time, and correlated well with large outbreaks of COVID-19 in the state. Conversely, attendances of people with diabetes to GP clinics, especially using VC services, increased substantially over the pandemic period. Discussion During the pandemic there was a substantial avoidance of hospital care by patients with diabetes. However, this may have been replaced by VC offered in the community for those with less severe diseases.
Impact of Kidney Function and Kidney Volume on Intracranial Aneurysms in Patients with Autosomal Dominant Polycystic Kidney Disease
Authors: Kataoka Hiroshi , Akagawa Hiroyuki , Yoshida Rie , Iwasa Naomi , Ushio Yusuke , Akihisa Taro , Sato Masayo , Manabe Shun , Makabe Shiho , Kawachi Keiko , Hoshino Junichi , Tsuchiya Ken , Nitta Kosaku , Mochizuki Toshio 
Published: June 2022

Abstract
Background
To date, only a personal or family history of intracranial aneurysm/subarachnoid hemorrhage (IA/SAH) has been established as a risk factor for IA in autosomal dominant polycystic kidney disease (ADPKD). Recently, an association between splicing/frameshift mutations, which are reportedly associated with declining kidney function/increased total kidney volume (TKV), and IAs in patients with ADPKD was reported.
Objective
To confirm the association between kidney function/volume and IAs in patients with ADPKD.
Methods
This study included 519 patients with ADPKD. Kidney function, TKV, and general risk factors for IA were assessed for associations with IA formation.
Results
At baseline IA screening, the median age, estimated glomerular filtration rate, and TKV were 44 years, 54.5 mL/min/1.73 m2, and 1054.3 mL, respectively. A family IA/SAH history was confirmed in 18.1% patients, and 54.3% patients had hypertension. IA point prevalence was 12.5%. During a clinical follow-up of 3104 patient-years, de novo IA was detected in 29 patients (0.93% patient-years). IA period prevalence was 18.1% (median age, 60 years). Multivariable logistic regression analyses demonstrated that TKV ≥ 1000 mL (odds ratio [OR] = 2.81), height-adjusted TKV ≥ 500 mL (OR = 2.81), Mayo 1D–1E (OR = 2.52), and chronic kidney disease stages 3–5 (OR = 2.31) were significantly associated with IA formation.
Conclusions
IAs in patients with ADPKD may be associated with risk factors for IAs in the general population as well as declining kidney function and increased kidney volume. Kidney disease progression may contribute to effective IA screening and treatment planning in patients with ADPKD.
Visualizing bacterial quorum sensing and quorum quenching interaction on Medicago roots
Authors: Rosier Amanda , Bais Harsh P. 
Published: June 2022

Abstract
Background
Defining interactions of bacteria in the rhizosphere (area near and on the plant root) is important to understand how they affect plant health. Some rhizosphere bacteria, including plant growth promoting rhizobacteria (PGPR) engage in the intraspecies communication known as quorum sensing (QS). Many use the extracellular autoinducer signal molecule N-acyl homoserine lactone (AHL) and other rhizobacteria, including PGPR, can interfere or disrupt QS through quorum quenching (QQ). Traditional AHL biosensor assays used for screening and identifying QS and QQ bacteria interactions fail to account for the role of the plant root.
Methods
Medicago spp. seedlings germinated on Lullien’s agar are transferred to soft-agar plates containing the broad-range AHL biosensor Agrobacterium tumefaciens KYC55 and X-gal substrate. Cultures of QS and QQ bacteria as well as pure AHLs and QQ protein are applied to the plant roots and incubated for 3 days.
Results
We show that this expanded use of an AHL biosensor successfully allowed for visualization of QS/QQ interactions localized at the plant root. KYC55 detected pure AHLs as well as AHLs from live bacteria cultures grown directly on the media. We also showed clear detection of QQ interactions occurring in the presence of the plant root.
Conclusions
Our novel tri-trophic system using an AHL biosensor is useful to study QS interspecies interactions in the rhizosphere.
Expression of hexokinase-2 (HK2), glutaminase-1 (GLS1) and fatty acid synthase (FASN) in gastric cancer and their prognostic significance
Authors: García-Martínez Elisa , Lino-Silva Leonardo S. , Romo-Pérez Adriana , Chavez-Blanco Alma , Dominguez-Gomez Guadalupe , Taja-Chayeb Lucia , Lopez-Basave Horacio N , Padilla-Rosciano Alejandro E , Diaz-Romero Consuelo , Trejo-Becerril Catalina , González-Fierro Aurora , Duenas-González Alfonso 
Published: June 2022

Abstract
PurposeThe malignant metabolic phenotype characterized by high rates of glycolysis, glutaminolysis and de novo synthesis of fatty acids is also observed in gastric carcinoma. These alterations can be used as therapeutical opportunities with the use of drug inhibitors targeting key enzymes for the metabolism of glucose, glutamine and fatty acid synthesis. MethodsHere we analyzed the immunohistochemical expression of Hexokinase-2 (HK2), Glutaminase-1 (GLS1) and fatty acid synthase (FASN) key enzymes for glycolysis, glutaminolysis and de novo fatty acid synthesis in 92 tumor samples of diffuse gastric carcinoma. Clinicopathological variables, treatment and follow-up data were obtained from files. ResultsHK2 overexpressed in 19 (20.7%), GLS1 in 28 (30.4%) and FASN in 21 (22.8%). Ten (10.9%) patients co-overexpressed HK2/FASN and 8 (8.7%) patients the three enzymes (HK2/GLS1/FASN). Enzyme overexpression either single or combined but no GLS1, correlated with higher stage and disease progression. At a median follow-up time of 31.8 months (2-116). All patients with combined expression died. The co-overexpression of both HK2/FASN and HK2/GLS/FASN was associated with shorter progression free survival whereas only the triple expression with overall survival (median 11.1 vs. 20.4, p=0.01). Nonetheless, stage was the only independent variable for overall survival. ConclusionIn our knowledge, this is the first study to demonstrate that HK2, GLS1 and FASN are concurrently overexpressed which provide support for further studies evaluating the antitumor effects of inhibitors of glycolysis, glutaminolysis and de novo synthesis of fatty acids for metabolic therapy of gastric cancer.
Diffuse alveolar hemorrhage caused by sevoflurane: case report and literature review
Authors: Zhu Yao , Shang Guansheng , Qin Yiwei , Gu Shengyan , Wang Fengling , Huang Jin 
Published: June 2022

Abstract
Diffuse alveolar hemorrhage (DAH), as an exclusion diagnosis in the clinic, is often occult but with serious consequences. DAH can be caused by many immune factors such as systemic lupus erythematosus (SLE) and antiphospholipid syndrome, or non-immune factors such as cocaine, marijuana, infection and radiotherapy. This paper reports a case of DAH caused by sevoflurane during anesthesia. The patient was a 31-year-old healthy male. After the oxygenation decreased during operation, the resuscitation was delayed in the anesthesia resuscitation room. At the same time, severe acute cor pulmonale occurred, which caused hemodynamic disorder and a progressive decrease in oxygenation. After active treatment, the patient recovered. In addition, DAH caused by sevoflurane and other factors was reviewed in this paper.
The Impact of Pathogenic BRCA1/2 Tumor Mutation Status on Advanced Stage-High Grade Serous Epithelial Ovarian Cancer Survival Outcome
Authors: Sutrisno Sutrisno , Purbadi Sigit , Tjahjadi Hartono , Werdhani Retno Asti , Khonsa Oni , Sembiring Ruth Emalian , Effendi Iwan Kurnia , Gultom Fajar Lamhot , Marleen Stella , Sugiharto Sony , Hendrasti Ardiana , Maharani Nastiti 
Published: June 2022

Abstract
Objective: To evaluate the impact of pathogenic BRCA1/2 tumor mutation on advanced stage-high grade serous epithelial ovarian cancer (HGSOC) survival outcome. Methods: A total of 68 from 144 patients were diagnosed with FIGO 2014 stage IIB-IV HGSOC between January 1st 2015, until March 31st 2021, at Dr. Cipto Mangunkusumo National Central Referral Hospital, Persahabatan Central Referral Hospital, and MRCCC Central Referral Hospital Siloam Jakarta, underwent NGS tumor BRCA1/2 gene testing and were included in this cohort historical study. We compared patient’s overall survival outcomes to pathogenic BRCA1/2 tumor mutational status. The patient’s clinicopathological characteristic factors that might affect patient’s survival outcomes were also investigated. Results: In the group with pathogenic BRCA1/2 tumor mutation, the likelihood of dying was 86% ​​lower (adjusted RR 0.149; 95%CI 0.046-0.475; p-value=0.001), and the median survival was better (median 46 months; 95%CI 34.009-57.991; p value=0.001) than the group without pathogenic BRCA1/2 tumor mutations (median 23 months; 95%CI 15.657-30.343; p value=0.001). The multivariate analyses identified pathogenic BRCA1/2 tumor mutation as an independent favorable prognostic factor for survival outcome (adjusted RR 0.149; 95%CI 0.046-0.475; p-value=0.001).Conclusions: In advanced stage-HGSOC patients, the pathogenic BRCA1/2 tumor mutations group have a better prognosis with longer survival outcomes than those without pathogenic BRCA1/2 tumor mutations.
Early Signs of Neuroinflammation in the Postnatal Wobbler Mice Model of Amyotrophic Lateral Sclerosis
Authors: Meyer Maria , Lima Analia , Deniselle Maria Claudia Gonzalez , De Nicola Alejandro F. 
Published: June 2022

Abstract
The Wobbler mouse is an accepted model of sporadic amyotrophic lateral sclerosis. The spinal cord of clinically symptomatic animals (3-5 months old) show vacuolar motoneuron degeneration, inflammation and gliosis accompanied by motor impairment. However, data are not conclusive concerning pathological changes appearing early after birth. To answer this question, we used postnatal day (PND) 6 genotyped Wobbler pups to determine abnormalities of glia and neurons at this early age period. We found astrogliosis, microgliosis with morphophenotypic changes pointing to active ameboid microglia, enhanced expression of the proinflammatory markers TLR4, NFkB, TNFα and inducible nitric oxide synthase. The astrocytic enzyme glutamine synthase and the glutamate-aspartate transporter GLAST were also reduced in PND 6 Wobbler pups, suggesting excitotoxicity due to impaired glutamate homeostasis. At the neuronal level, PND 6 Wobblers showed swollen soma, increased choline acetyltransferase immunofluorescence staining and low expression of the neuronal nuclear antigen NeuN. However, vacuolated motoneurons, a typical signature of older clinically symptomatic Wobbler mice were absent in the spinal cord of PND 6 Wobblers. The results suggest predominance of neuroinflammation and abnormalities of microglia and astrocytes at this early period of Wobbler life, accompanied by some neuronal changes. Data support the non-cell autonomous hypothesis of the Wobbler disorder, and bring useful information with regards to intervening molecular inflammatory mechanisms at the beginning stage of human motoneuron degenerative diseases.