bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decoherence of V$${}_{{{{{{{{\rm{B}}}}}}}}}^{-}$$ spin defects in monoisotopic hexagonal boron nitride
Authors: Haykal A. , Tanos R. , Minotto N. , Durand A. , Fabre F. , Li J. , Edgar J. H. , Ivády V. , Gali A. , Michel T. , Dréau A. , Gil B. , Cassabois G. , Jacques V. 
Published: July 2022

AbstractSpin defects in hexagonal boron nitride (hBN) are promising quantum systems for the design of flexible two-dimensional quantum sensing platforms. Here we rely on hBN crystals isotopically enriched with either 10B or 11B to investigate the isotope-dependent properties of a spin defect featuring a broadband photoluminescence signal in the near infrared. By analyzing the hyperfine structure of the spin defect while changing the boron isotope, we first confirm that it corresponds to the negatively charged boron-vacancy center ($${{{{{{{{\rm{V}}}}}}}}}_{{{{{{{{\rm{B}}}}}}}}}^{-}$$

V

B

−

). We then show that its spin coherence properties are slightly improved in 10B-enriched samples. This is supported by numerical simulations employing cluster correlation expansion methods, which reveal the importance of the hyperfine Fermi contact term for calculating the coherence time of point defects in hBN. Using cross-relaxation spectroscopy, we finally identify dark electron spin impurities as an additional source of decoherence. This work provides new insights into the properties of $${{{{{{{{\rm{V}}}}}}}}}_{{{{{{{{\rm{B}}}}}}}}}^{-}$$

V

B

−

spin defects, which are valuable for the future development of hBN-based quantum sensing foils.
FLASHIda enables intelligent data acquisition for top–down proteomics to boost proteoform identification counts
Authors: Jeong Kyowon , Babović Maša , Gorshkov Vladimir , Kim Jihyung , Jensen Ole N. , Kohlbacher Oliver 
Published: July 2022

AbstractThe detailed analysis and structural characterization of proteoforms by top-down proteomics (TDP) has gained a lot of interest in biomedical research. Data-dependent acquisition (DDA) of intact proteins is non-trivial due to the diversity and complexity of proteoforms. Dedicated acquisition methods thus have the potential to greatly improve TDP. Here, we present FLASHIda, an intelligent online data acquisition algorithm for TDP that ensures the real-time selection of high-quality precursors of diverse proteoforms. FLASHIda combines fast charge deconvolution algorithms and machine learning-based quality assessment for optimal precursor selection. In an analysis ofE. colilysate, FLASHIda increases the number of unique proteoform level identifications from 800 to 1500 or generates a near-identical number of identifications in one third of the instrument time when compared to standard DDA mode. Furthermore, FLASHIda enables sensitive mapping of post-translational modifications and detection of chemical adducts. As a software extension module to the instrument, FLASHIda can be readily adopted for TDP studies of complex samples to enhance proteoform identification rates.
Sustainable oxygen evolution electrocatalysis in aqueous 1 M H2SO4 with earth abundant nanostructured Co3O4
Authors: Yu Jiahao , Garcés-Pineda Felipe A. , González-Cobos Jesús , Peña-Díaz Marina , Rogero Celia , Giménez Sixto , Spadaro Maria Chiara , Arbiol Jordi , Barja Sara , Galán-Mascarós José Ramón 
Published: July 2022

AbstractEarth-abundant electrocatalysts for the oxygen evolution reaction (OER) able to work in acidic working conditions are elusive. While many first-row transition metal oxides are competitive in alkaline media, most of them just dissolve or become inactive at high proton concentrations where hydrogen evolution is preferred. Only noble-metal catalysts, such as IrO2, are fast and stable enough in acidic media. Herein, we report the excellent activity and long-term stability of Co3O4-based anodes in 1 M H2SO4 (pH 0.1) when processed in a partially hydrophobic carbon-based protecting matrix. These Co3O4@C composites reliably drive O2 evolution a 10 mA cm–2 current density for >40 h without appearance of performance fatigue, successfully passing benchmarking protocols without incorporating noble metals. Our strategy opens an alternative venue towards fast, energy efficient acid-media water oxidation electrodes.
A reconfigurable and magnetically responsive assembly for dynamic solar steam generation
Authors: Hu Yajie , Ma Hongyun , Wu Mingmao , Lin Tengyu , Yao Houze , Liu Feng , Cheng Huhu , Qu Liangti 
Published: July 2022

AbstractInterfacial solar vapor generation is a promising technique to efficiently get fresh water from seawater or effluent. However, for the traditional static evaporation models, further performance improvement has encountered bottlenecks due to the lack of dynamic management and self-regulation on the evolving water movement and phase change in the evaporation process. Here, a reconfigurable and magnetically responsive evaporator with conic arrays is developed through the controllable and reversible assembly of graphene wrapped Fe3O4nanoparticles. Different from the traditional structure-rigid evaporation architecture, the deformable and dynamic assemblies could reconfigure themselves both at macroscopic and microscopic scales in response to the variable magnetic field. Thus, the internal water transportation and external vapor diffusion are greatly promoted simultaneously, leading to a 23% higher evaporation rate than that of static counterparts. Further, well-designed hierarchical assembly and dynamic evaporation system can boost the evaporation rate to a record high level of 5.9 kg m−2h−1. This proof-of-concept work demonstrates a new direction for development of high performance water evaporation system with the ability of dynamic reconfiguration and reassembly.
Copper-catalyzed Z-selective synthesis of acrylamides and polyacrylamides via alkylidene ketenimines
Authors: Duan Xuelun , Zheng Nan , Li Ming , Liu Gongbo , Sun Xinhao , Wu Qiming , Song Wangze 
Published: July 2022

AbstractIt remains very important to discover and study new fundamental intermediates consisting of carbon and nitrogen as the abundant elements of organic molecules. The unique alkylidene ketenimine could be formed in situ under mild conditions by an unexpected copper-catalyzed three-component reaction of alkyne, azide and water involving a successive cycloaddition, N2 extrusion and carbene-assisted rearrangement. Only Z-α,β-unsaturated amides instead of E-α,β-unsaturated amides or triazoles were acquired from alkylidene ketenimines with excellent selectivities and stereospecificities. In addition, a series of “approximate” alternating copolymers (poly (triazole-alt-Z-acrylamides)) with high Mns and yields were efficiently afforded by multicomponent polymerization through a very simple operation basing on this multicomponent reaction.
T cell receptor β-chain-targeting chimeric antigen receptor T cells against T cell malignancies
Authors: Li Fanlin , Zhang Huihui , Wang Wanting , Yang Puyuan , Huang Yue , Zhang Junshi , Yan Yaping , Wang Yuan , Ding Xizhong , Liang Jie , Qi Xinyue , Li Min , Han Ping , Zhang Xiaoqing , Wang Xin , Cao Jiang , Fu Yang-Xin , Yang Xuanming 
Published: July 2022

AbstractThe success of chimeric antigen receptor (CAR) T cells in treating B cell malignancies comes at the price of eradicating normal B cells. Even though T cell malignancies are aggressive and treatment options are limited, similar strategies for T cell malignancies are constrained by the severe immune suppression arising from bystander T cell aplasia. Here, we show the selective killing of malignant T cells without affecting normal T cell-mediated immune responses in vitro and in a mouse model of disseminated leukemia. Further, we develop a CAR construct that carries the single chain variable fragment of a subtype-specific antibody against the variable TCR β-chain region. We demonstrate that these anti-Vβ8 CAR-T cells are able to recognize and kill all Vβ8+malignant T cells that arise from clonal expansion while sparing malignant or healthy Vβ8−T cells, allowing sufficient T cell-mediated cellular immunity. In summary, we present a proof of concept for a selective CAR-T cell therapy to eradicate T cell malignancies while maintaining functional adaptive immunity, which opens the possibility for clinical development.
An acquired phosphatidylinositol 4-phosphate transport initiates T-cell deterioration and leukemogenesis
Authors: Zhong Wenbin , Lin Weize , Yang Yingjie , Chen Dan , Cao Xiuye , Xu Mengyang , Pan Guoping , Chen Huanzhao , Zheng Jie , Feng Xiaoqin , Yang Li hua , Lai Chaofeng , Olkkonen Vesa M. , Xu Jun , Cui Shuzhong , Yan Daoguang 
Published: July 2022

AbstractLipid remodeling is crucial for malignant cell transformation and tumorigenesis, but the precise molecular processes involved and direct evidences for these in vivo remain elusive. Here, we report that oxysterol-binding protein (OSBP)-related protein 4 L (ORP4L) is expressed in adult T-cell leukemia (ATL) cells but not normal T-cells. In ORP4L knock-in T-cells, ORP4L dimerizes with OSBP to control the shuttling of OSBP between the Golgi apparatus and the plasma membrane (PM) as an exchanger of phosphatidylinositol 4-phosphate [PI(4)P]/cholesterol. The PI(4)P arriving at the PM via this transport machinery replenishes phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and phosphatidylinositol (3,4,5) trisphosphate [PI(3,4,5)P3] biosynthesis, thus contributing to PI3K/AKT hyperactivation and T-cell deterioration in vitro and in vivo. Disruption of ORP4L and OSBP dimerization disables PI(4)P transport and T-cell leukemogenesis. In summary, we identify a non-vesicular lipid transport machinery between Golgi and PM maintaining the oncogenic signaling competence initiating T-cell deterioration and leukemogenesis.
Proteotoxicity caused by perturbed protein complexes underlies hybrid incompatibility in yeast
Authors: Swamy Krishna B. S. , Lee Hsin-Yi , Ladra Carmina , Liu Chien-Fu Jeff , Chao Jung-Chi , Chen Yi-Yun , Leu Jun-Yi 
Published: July 2022

AbstractDobzhansky–Muller incompatibilities represent a major driver of reproductive isolation between species. They are caused when interacting components encoded by alleles from different species cannot function properly when mixed. At incipient stages of speciation, complex incompatibilities involving multiple genetic loci with weak effects are frequently observed, but the underlying mechanisms remain elusive. Here we show perturbed proteostasis leading to compromised mitosis and meiosis inSaccharomyces cerevisiaehybrid lines carrying one or two chromosomes fromSaccharomyces bayanusvar.uvarum. Levels of proteotoxicity are correlated with the number of protein complexes on replaced chromosomes. Proteomic approaches reveal that multi-protein complexes with subunits encoded by replaced chromosomes tend to be unstable. Furthermore, hybrid defects can be alleviated or aggravated, respectively, by up- or down-regulating the ubiquitin-proteasomal degradation machinery, suggesting that destabilized complex subunits overburden the proteostasis machinery and compromise hybrid fitness. Our findings reveal the general role of impaired protein complex assembly in complex incompatibilities.
Functional neuronal circuitry and oscillatory dynamics in human brain organoids
Authors: Sharf Tal , van der Molen Tjitse , Glasauer Stella M. K. , Guzman Elmer , Buccino Alessio P. , Luna Gabriel , Cheng Zhuowei , Audouard Morgane , Ranasinghe Kamalini G. , Kudo Kiwamu , Nagarajan Srikantan S. , Tovar Kenneth R. , Petzold Linda R. , Hierlemann Andreas , Hansma Paul K. , Kosik Kenneth S. 
Published: July 2022

AbstractHuman brain organoids replicate much of the cellular diversity and developmental anatomy of the human brain. However, the physiology of neuronal circuits within organoids remains under-explored. With high-density CMOS microelectrode arrays and shank electrodes, we captured spontaneous extracellular activity from brain organoids derived from human induced pluripotent stem cells. We inferred functional connectivity from spike timing, revealing a large number of weak connections within a skeleton of significantly fewer strong connections. A benzodiazepine increased the uniformity of firing patterns and decreased the relative fraction of weakly connected edges. Our analysis of the local field potential demonstrate that brain organoids contain neuronal assemblies of sufficient size and functional connectivity to co-activate and generate field potentials from their collective transmembrane currents that phase-lock to spiking activity. These results point to the potential of brain organoids for the study of neuropsychiatric diseases, drug action, and the effects of external stimuli upon neuronal networks.
Fatigue-free artificial ionic skin toughened by self-healable elastic nanomesh
Authors: Wang Jiqiang , Wu Baohu , Wei Peng , Sun Shengtong , Wu Peiyi 
Published: July 2022

AbstractRobust ionic sensing materials that are both fatigue-resistant and self-healable like human skin are essential for soft electronics and robotics with extended service life. However, most existing self-healable artificial ionic skins produced on the basis of network reconfiguration suffer from a low fatigue threshold due to the easy fracture of low-energy amorphous polymer chains with susceptible crack propagation. Here we engineer a fatigue-free yet fully healable hybrid ionic skin toughened by a high-energy, self-healable elastic nanomesh, resembling the repairable nanofibrous interwoven structure of human skin. Such a design affords a superhigh fatigue threshold of 2950 J m−2 while maintaining skin-like compliance, stretchability, and strain-adaptive stiffening response. Moreover, nanofiber tension-induced moisture breathing of ionic matrix leads to a record-high strain-sensing gauge factor of 66.8, far exceeding previous intrinsically stretchable ionic conductors. This concept creates opportunities for designing durable ion-conducting materials that replicate the unparalleled combinatory properties of natural skins more precisely.
Cascaded dissipative DNAzyme-driven layered networks guide transient replication of coded-strands as gene models
Authors: Wang Jianbang , Li Zhenzhen , Willner Itamar 
Published: July 2022

AbstractDynamic, transient, out-of-equilibrium networks guide cellular genetic, metabolic or signaling processes. Designing synthetic networks emulating natural processes imposes important challenges including the ordered connectivity of transient reaction modules, engineering of the appropriate balance between production and depletion of reaction constituents, and coupling of the reaction modules with emerging chemical functions dictated by the networks. Here we introduce the assembly of three coupled reaction modules executing a cascaded dynamic process leading to the transient formation and depletion of three different Mg2+-ion-dependent DNAzymes. The transient operation of the DNAzyme in one layer triggers the dynamic activation of the DNAzyme in the subsequent layer, leading to a three-layer transient catalytic cascade. The kinetics of the transient cascade is computationally simulated. The cascaded network is coupled to a polymerization/nicking DNA machinery guiding transient synthesis of three coded strands acting as “gene models”, and to the rolling circle polymerization machinery leading to the transient synthesis of fluorescent Zn(II)-PPIX/G-quadruplex chains or hemin/G-quadruplex catalytic wires.
Electronic and magnetic properties of the RuX3 (X = Cl, Br, I) family: two siblings—and a cousin?
Authors: Kaib David A. S. , Riedl Kira , Razpopov Aleksandar , Li Ying , Backes Steffen , Mazin Igor I. , Valentí Roser 
Published: July 2022

AbstractMotivated by reports of metallic behavior in the recently synthesized RuI3, in contrast to the Mott-insulating nature of the actively discussed α-RuCl3, as well as RuBr3, we present a detailed comparative analysis of the electronic and magnetic properties of this family of trihalides. Using a combination of first-principles calculations and effective-model considerations, we conclude that RuI3, similarly to the other two members, is most probably on the verge of a Mott insulator, but with much smaller magnetic moments and strong magnetic frustration. We predict the ideal pristine crystal of RuI3 to have a nearly vanishing conventional nearest-neighbor Heisenberg interaction and to be a quantum spin liquid candidate of a possibly different kind than the Kitaev spin liquid. In order to understand the apparent contradiction to the reported resistivity ρ, we analyze the experimental evidence for all three compounds and propose a scenario for the observed metallicity in existing samples of RuI3. Furthermore, for the Mott insulator RuBr3, we obtain a magnetic Hamiltonian of a similar form to that in the much-discussed α-RuCl3 and show that this Hamiltonian is in agreement with experimental evidence in RuBr3.
Mucosal administration of a live attenuated recombinant COVID-19 vaccine protects nonhuman primates from SARS-CoV-2
Authors: Tioni Mariana F. , Jordan Robert , Pena Angie Silva , Garg Aditya , Wu Danlu , Phan Shannon I. , Weiss Christopher M. , Cheng Xing , Greenhouse Jack , Orekov Tatyana , Valentin Daniel , Kar Swagata , Pessaint Laurent , Andersen Hanne , Stobart Christopher C. , Bloodworth Melissa H. , Stokes Peebles R. , Liu Yang , Xie Xuping , Shi Pei-Yong , Moore Martin L. , Tang Roderick S. 
Published: July 2022

AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the COVID-19 global pandemic. SARS-CoV-2 is an enveloped RNA virus that relies on its trimeric surface glycoprotein spike for entry into host cells. Here we describe the COVID-19 vaccine candidate MV-014-212, a live, attenuated, recombinant human respiratory syncytial virus expressing a chimeric SARS-CoV-2 spike as the only viral envelope protein. MV-014-212 was attenuated and immunogenic in African green monkeys (AGMs). One mucosal administration of MV-014-212 in AGMs protected against SARS-CoV-2 challenge, reducing by more than 200-fold the peak shedding of SARS-CoV-2 in the nose. MV-014-212 elicited mucosal immunoglobulin A in the nose and neutralizing antibodies in serum that exhibited cross-neutralization against virus variants of concern Alpha, Beta, and Delta. Intranasally delivered, live attenuated vaccines such as MV-014-212 entail low-cost manufacturing suitable for global deployment. MV-014-212 is currently in Phase 1 clinical trials as an intranasal COVID-19 vaccine.
Vaccination with Mycoplasma pneumoniae membrane lipoproteins induces IL-17A driven neutrophilia that mediates Vaccine-Enhanced Disease
Authors: Mara Arlind B. , Gavitt Tyler D. , Tulman Edan R. , Miller Jeremy M. , He Wu , Reinhardt Emily M. , Ozyck R. Grace , Goodridge Meagan L. , Silbart Lawrence K. , Szczepanek Steven M. , Geary Steven J. 
Published: July 2022

AbstractBacterial lipoproteins are an often-underappreciated class of microbe-associated molecular patterns with potent immunomodulatory activity. We previously reported that vaccination of BALB/c mice withMycoplasma pneumoniae(Mp) lipid-associated membrane proteins (LAMPs) resulted in lipoprotein-dependent vaccine enhanced disease after challenge with virulentMp, though the immune responses underpinning this phenomenon remain poorly understood. Herein, we report that lipoprotein-induced VED in a mouse model is associated with elevated inflammatory cytokines TNF-α, IL-1β, IL-6, IL-17A, and KC in lung lavage fluid and with suppurative pneumonia marked by exuberant neutrophilia in the pulmonary parenchyma. Whole-lung-digest flow cytometry and RNAScope analysis identified multiple cellular sources for IL-17A, and the numbers of IL-17A producing cells were increased in LAMPs-vaccinated/Mp-challenged animals compared to controls. Specific IL-17A or neutrophil depletion reduced disease severity in our VED model—indicating thatMplipoproteins induce VED in an IL-17A-dependent manner and through exuberant neutrophil recruitment. IL-17A neutralization reduced levels of TNF-α, IL-1β, IL-6, and KC, indicating that IL-17A preceded other inflammatory cytokines. Surprisingly, we found that IL-17A neutralization impaired bacterial clearance, while neutrophil depletion improved it—indicating that, while IL-17A appears to confer both maladaptive and protective responses, neutrophils play an entirely maladaptive role in VED. Given that lipoproteins are found in virtually all bacteria, the potential for lipoprotein-mediated maladaptive inflammatory responses should be taken into consideration when developing vaccines against bacterial pathogens.
A high spatial resolution land surface phenology dataset for AmeriFlux and NEON sites
Authors: Moon Minkyu , Richardson Andrew D. , Milliman Thomas , Friedl Mark A. 
Published: July 2022

AbstractVegetation phenology is a key control on water, energy, and carbon fluxes in terrestrial ecosystems. Because vegetation canopies are heterogeneous, spatially explicit information related to seasonality in vegetation activity provides valuable information for studies that use eddy covariance measurements to study ecosystem function and land-atmosphere interactions. Here we present a land surface phenology (LSP) dataset derived at 3 m spatial resolution from PlanetScope imagery across a range of plant functional types and climates in North America. The dataset provides spatially explicit information related to the timing of phenophase changes such as the start, peak, and end of vegetation activity, along with vegetation index metrics and associated quality assurance flags for the growing seasons of 2017–2021 for 10 × 10 km windows centred over 104 eddy covariance towers at AmeriFlux and National Ecological Observatory Network (NEON) sites. These LSP data can be used to analyse processes controlling the seasonality of ecosystem-scale carbon, water, and energy fluxes, to evaluate predictions from land surface models, and to assess satellite-based LSP products.
Design, synthesis, in vitro biological assessment and molecular modeling insights for novel 3-(naphthalen-1-yl)-4,5-dihydropyrazoles as anticancer agents with potential EGFR inhibitory activity
Authors: Eldehna Wagdy M. , El Hassab Mahmoud A. , Elsayed Zainab M. , Al-Warhi Tarfah , Elkady Hazem , Abo-Ashour Mahmoud F. , Abourehab Mohammed A. S. , Eissa Ibrahim H. , Abdel-Aziz Hatem A. 
Published: July 2022

AbstractCurrently, the humanity is in a fierce battle against various health-related challenges especially those associated with human malignancies. This created the urge to develop potent and selective inhibitors for tumor cells through targeting specific oncogenic proteins possessing crucial roles in cancer progression and survive. In this respect, new series of pyrazole-thiazol-4-one hybrids (9a–p) were synthesized as potential anticancer agents. All the synthesized molecules exhibited potent antiproliferative actions against breast cancer (BC) T-47D and MDA-MB-231 cell lines with IC50 ranges 3.14–4.92 and 0.62–58.01, respectively. Moreover, the most potent anti-proliferative counterparts 9g and 9k were assessed against EGFR. They displayed nanomolar inhibitory activity, IC50 267 ± 12 and 395 ± 17 nM, respectively. Worth noting, both compounds 9g and 9k induced apoptosis in MDA-MB-231 cells, and resulted in a cell cycle arrest at G2/M phase. Furthermore, an in silico analysis including docking and molecular dynamic simulations was performed.