bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Water Yield Pattern for Annual and Monthly Scales Through a Unifying Catchment Water Balance Model
Authors: Liu Dedi , Fu Dezhi 
Published: January 2022

Abstract
Long-term scheduling and short-term decision-making for water resources management often require understanding the relationship of water yield pattern between the annual and monthly scales. As the water yield pattern mainly depends on land cover/use and climate, a unifying catchment water balance model with factors has been adopted to derive a theoretical water yield pattern with annual and monthly scales. Two critical values at the parameters ε=1-√2/2 and ϕ=1.0 are identified. The parameter ε referring to the water storage (land use/cover) and evaporation (climate) changes can make more contribution than ϕ for water yield when ϕ>1.0, especially with ε<1-√2/2. But there is less contribution made by ε when ϕ<1.0. The derived theoretical water yield patterns have also been validated by the observed data or the simulated data through the hydrological model. Due to the bias of the soil moisture data, a lot of the estimated parameter ε values are over its theoretical range, especially for the monthly scale in humid basins. The performance of the derived theoretical water yield pattern at annual scale is much better than that at monthly scale while there are only a few data sets from the arid basin at every months fall within their theoretical ranges. Even the relative contributions of ε is found to be bigger than those of ϕ due to ε<1-√2/2 and ϕ>1.0, there are no significant linear relationships between annual and monthly parameters ε and ϕ. Our results not only validate the derived theoretical water yield pattern with the estimated parameter directly by the observed or simulated data rather than the calibrated parameter, but also can guide for further understanding physical of water balance to conversion time scales for the combing long-term and short-term water resources management.
Securing the Environmental Water Requirements of Seasonally Ponding Wetlands: Partnering Science and Management Through Benefit Sharing
Authors: Barchiesi Stefano , Camacho Antonio , Hernández Eva , Guelmami Anis , Monti Flavio , Satta Alessio , Jordán Osvaldo , Angelini Christine 
Published: January 2022

Abstract
Although environmental flow regime assessments are becoming increasingly holistic, they rarely provoke water managers to enact the adaptive water reallocation mechanisms required to secure environmental water for wetlands. The conditions that cause science-based environmental flow assessments to succeed or fail in informing the management of environmental water requirements remain unclear. To begin to resolve these conditions, we used process tracing to deconstruct the sequence of activities required to manage environmental water in four case studies of seasonally ponding wetlands in Mediterranean and Mesoamerican watersheds. We hypothesized that, when the flexibility and equitability of the socioeconomic system do not match the complexity of the biophysical system, this leads to a failure of managers to integrate scientific guidance in their allocation of environmental water. Diagnostic evidence gathered indicates that science-management partnerships are essential to align institutional flexibility and socioeconomic equitability with the system’s ecohydrological complexity, and thus move from determination to reallocation of environmental water. These results confirm that institutions e.g., river basin organizations need to be supplemented by motivated actors with experience and skill to negotiate allocation and adaptive management of environmental water. These institutional-actor synergies are likely to be especially important in water scarce regions when the need to accommodate extreme hydrological conditions is not met by national governance capacity. We conclude by focusing on benefit sharing as a means to better describe the conditions for successful science-based environmental flow assessments that realize productive efficiency in environmental water allocation i.e., recognition of multiple values for both people and ecosystems.
Characterization of Groundwater Zones and Salinity Assessment in Aquifers of the Campo District, South Cameroon Using the GIS, AHP and PCA Approach.
Authors: Raoul Kouamou Njifen Serges , Francoise Eyengue A Nyam , Hermann Fossi Donald , Marcelin Bikoro Bi-Alou , Anita Ngouokouo Tchikangoua , Parfait Bounoung Clement , Tabod Tabod Charles 
Published: January 2022

Abstract
In the Campo region, groundwater is critical for human consumption and social activity. Groundwater potential is influenced by a region's geological, geophysical, and hydrogeological factors. The major goals of this research are to determine which regions are ideal for productive groundwater drilling and to assess the source of salinity in the study area's coastal aquifers. The groundwater potential map was created using Geographic Information Systems (GIS) and the Hierarchical Analysis Process (AHP). The process of groundwater mineralization was studied using principal component analysis (PCA). Six variables were taken into account, and weights were assigned to them based on their impact on groundwater recharge. In a GIS environment, spatial integration and a combination of theme layers were conducted. Campo's groundwater potential map was divided into four zones: low 14.4% (389.6 km²), moderate 53.3% (1484.5 km²), high 28.3% (783.3 km²), and extremely high 4.1% (110.9 km²). The results of the PCA reveal a mechanism of water-rock interaction, as a result of geological alteration and a salinization process caused by the intrusion of seawater and human activity The source of salinity in groundwater is manmade (agricultural and residential activities) rather than seawater intrusion. Seawater infiltration is not greatly aided by the low lineament density found near the beaches.
Gut Microbiota and Metabolome Alterations Associated with Chinese Monks’ Practice
Authors: Qiao Tingting , Lu Ganghua , Lv Zhongwei , Li Dan , Jia Chengyou , Song Yingchun , Jiang Wen , Yu Xiaqing , Gao Dingwei , Tong Junyu , Wang Ru 
Published: January 2022

Abstract
BackgroundThe practices of monks mainly include long-term vegetarianism and meditation, which are likely to fundamentally influence the gut microbiota and fecal metabolites. We aim to study the relationship between the practices of Chinese monks and gut microbiotas and metabolites.MethodsTwenty-four monks and forty-eight omnivorous controls (never meditated) were included. The microbiotas of all samples were profiled by 16S rRNA gene sequencing, and the metabolomes were examined by nontargeted LC–MS metabolomics. Twenty-four monks were divided into the H group and the L group according to the median time of practice, and microbiota and metabolite analyses were carried out in the two groups.ResultsMicrobial communities and metabolites were decreased in monks. Bacteroidetes was increased in monks, while the Firmicutes, Actinobacteria, and Firmicutes/Bacteroidetes ratios were decreased. At the genus level, Faecalibacterium, Lachnospira, Roseburia, norank_f__Lachnospiraceae, etc. were higher in monks, while Blautia, Eubacterium__hallii_group, Bifidobacteria, etc. were lower (all p < 0.05). Most identical KEGG categories in both Tax4Fun and PICRUSt2 were related to metabolism (6/8, 75.0%). Most higher abundance genera were positively correlated with higher abundance metabolites in monks, indicating that intestinal flora significantly affects intestinal metabolic function. Lipids and lipid-like molecules were the major differential metabolites (VIP >2, p < 0.05) in the two groups. L-dopa plays an important role in many metabolic pathways in monks. Prevotella_9 was enriched in the L group, while norank_f__Lachnospiraceae was enriched in the H group. DG (16:0/18:0/0:0) was highly expressed in the H group and participated in sixteen KEGG functional pathways as well as many immune-related KEGG enrichment pathways.Conclusion﻿The monks' lifestyle practices of vegetarianism and meditation have the potential to modulate human metabolism and function by affecting the gut microbial composition and metabolites. The appropriate practice of monks makes the intestine younger and increases immunity, but long-term practice may cause adverse physical and mental events.
Effect of Cu(II) and Conserved Copper Binding Sites on Multicopper Oxidase CopA and Characterization of the BioMnOx
Authors: Tang Wenwei , Jin Xiaoyu , Liu Yunying , Zhang Mengtian , Li Xiaoxuan , Zeng Xinping 
Published: January 2022

Abstract
The microbial manganese removal process is believed to be the catalytic oxidation of Mn(II) by manganese oxidase. In this study, the multicopper oxidase CopA was purified and found to have high manganese oxidation activity in vitro and Cu(II) can significantly enhance its manganese oxidation activity. The gene site-directed mutagenesis was used to mutate four conserved copper binding sites of CopA and then obtain four mutant strains. The manganese removal efficiency of the four strains was determined to find that H120 is the catalytic active site of the CopA. Protein modification analysis of CopA obtained under different conditions by mass spectrometry revealed that the loss of Cu(Ⅱ) and the mutation of the conserved copper binding site H120 resulted in the loss of modification of ethoxyformyl and quinone, the number of modifications was reduced and the position of modification was changed, eventually causing a decrease in protein activity. It reveals that Cu(II) and H120 play an indispensable role in the manganese oxidation of the multicopper oxidase CopA. The Mn valence state of BioMnOx was analyzed by XPS, finding that both the strain-mediated product and the CopA-mediated product were composed of MnO2 and Mn3O4 and the average valence of Mn is 3.2.
Prevalence of SARS-CoV-2 Antibodies and Risk Factors in the Pandemic Epicentre of Catalonia
Authors: Moreno Sandra , Miró Queralt , Soler Ainhoa , Gallego Mireia , Homs Maria , Garcia Maria José 
Published: January 2022

Abstract
Aim To define the seroprevalence of antibodies against SARS-CoV-2 in the municipality of Vilanova del Camí (in the sub-region of Conca d'Ódena) and to know the risk factors associated with positive seroprevalence.Method Cross-sectional descriptive study. The population of Vilanova del Camí had the opportunity to voluntarily attend two screenings for antibodies against SARS-CoV-2 (October and December 2020). Users who participated in the screening signed an informed consent form. From the 3610 attendees at the screening 2,170 patients were randomly selected. The test results and other demographic variables (sex, age, morbidity index) and users’ clinical variables (diagnoses, smoking and drugs) were analysed. Results The prevalence of antibodies against COVID19 was 9.6% (95% CI 8.4% to 10.9%) and was similar for men and women but increased with age. In complex chronic patients, the prevalence was 14.3% and in patients with advanced chronic disease, 25%. Age, AMG (Adjusted Morbidity Groups) index, COVID diagnosis and COVID contact diagnosis were risk factors for positive seroprevalence. The October analysis detected a higher seroprevalence (12.16%) than in the December analysis (8.38%). In the December screening, obesity was a risk factor for positive seroprevalence. Conclusions This study demonstrates the high seroprevalence of antibodies in the pandemic epicentre of Catalonia. The differences in the analysis of October and December show the impact of the first wave in the Conca d'Ódena sub-region.
Safety and efficacy of pegylated recombinant human granulocyte colony-stimulating factor during concurrent chemoradiotherapy for small-cell lung cancer: a retrospective, cohort-controlled trial
Authors: Wang Cunliang , Zhu Shouhui , Miao Chuanwang , Wang Yu , Chen Jiazhen , Yuan Shuanghu , Hu Xudong 
Published: January 2022

Abstract
Objective

To investigate pegylated recombinant human granulocyte colony-stimulating factor (PEG-rhG-CSF) safety and efficacy in preventing hematological toxicity during concurrent chemoradiotherapy (CCRT) for small-cell lung cancer (SCLC).
Methods

We retrospectively assessed 80 SCLC patients treated with CCRT from January 2013 to December 2018 who received PEG-rhG-CSF within 48 hours after the end of chemotherapy, defined as prophylactic use, as the experimental group. An additional 80 patients who were not treated with PEG-rhG-CSF were matched 1:1 by the propensity score matching method and served as the control group. The main observations were differences in hematological toxicity, neutrophil changes, febrile neutropenia (FN) incidence and adverse reactions. Progression-free survival (PFS) and overall survival (OS) were analyzed with regular assessment and follow-up.
Results

The leukocyte, neutrophil, erythrocyte, and platelet counts and hemoglobin level decreased after CCRT, but the experimental group had slightly higher leukocyte and neutrophil counts than the control group (P<0.05). The incidences of grade III-IV leukopenia (18.75% vs. 61.25%) and neutropenia (23.75% vs. 67.5%) in the experimental group were significantly lower than those in the control group (P<0.05). The absolute neutrophil count was 4.17±0.79 on day 1 and peaked (6.81±2.37) on day 10 in the experimental group; the value in the control group was 2.81±0.86 on day 1. It decreased significantly and reached the minimum (0.91±0.53) on day 10 (P<0.05). The experimental group had a lower FN incidence than the control group (P<0.05). There was also no significant acute esophagitis or pulmonary toxicity. The treatment had no significant effect on PFS (11.4 vs. 8.7, P=0.958) or OS (23.9 vs. 17.3, P=0.325) over an 18.6-month median follow-up time.
Conclusion

PEG-rhG-CSF has good efficacy and safety in preventing hematological toxicity in SCLC patients during CCRT and has no significant effects on PFS or OS.
Genetic and QTL Analysis for Hilum-Eye Types in Cowpea (Vigna Unguiculata L. Walp)
Authors: Angira Brijesh , Zhang Yang , Zhang Hong-Bin , Zhang Meiping , Singh B.B. , Hays Dirk B 
Published: January 2022

Abstract
Cowpea is an important food legume widely grown in the semi-arid tropics and serves as a main source of dietary protein, minerals, and vitamins. However, varieties differ from region to region based on the consumer’s preference for seed types determined by seed size, seed coat texture, seed color, and hilum-eye types. The genetics of seed size, seed color, and seed coat texture have been well documented, but the hilum-eye types have not been studied well because they represent seven different types with complex interactions. We studied the genetic segregation for hilum-eye types and determined the number of genes involved in a recombinant inbred line (RIL) population derived from a cross between a small eye parent ‘GEC’ and a Watson eye parent ‘IT98K-476-8’. The results demonstrated a three-gene model, W (Watson), S (small), and R (large), for cowpea seed hilum-eye type pattern and the interaction of these three genes, W, S, and R, resulted in five phenotypes, viz. self, Watson, small, large, and ring hilum-eye types. Moreover, we also mapped the RILs for hilum-eye types, identified three quantitative trait loci (QTLs), and aligned to the cowpea reference genome as QTL qHilum7.1, qHilum9.1, and qHilum10.1, corresponding to these three genes, Ring type (R), Watson type (W), and Small type (S) hilum-eye type patterns, respectively. Therefore, there was a complete agreement between the genetic analysis and QTL mapping for the number of genes controlling the hilum types in cowpea.
Using Bayesian state-space models to understand the population dynamics of the dominant malaria vector, Anopheles funestus in rural Tanzania
Authors: Ngowo Halfan , Okumu Fredros Oketch , Hape Emmanuel Elirehema , Mshani Issa H , Ferguson Heather M , Matthiopoulos Jason 
Published: January 2022

Abstract
Background: It is often assumed that the population dynamics of the malaria vector Anopheles funestus, its role in malaria transmission and the way it responds to interventions are similar to the more elaborately characterized An. gambiae. However, An. funestus has several unique ecological features that could generate distinct transmission dynamics and responsiveness to interventions. The objectives of this work were to develop a model which will; 1) reconstruct the population dynamics, survival, and fecundity of wild An. funestus populations in southern Tanzania, 2) quantify impacts of density dependence on the dynamics, and 3) assess seasonal fluctuations in An. funestus demography. Through quantifying the population dynamics of An. funestus, this model will enable analysis of how their stability and response to interventions may different from that of An. gambiae s.l.Methods: A Bayesian State Space Model (SSM) based on mosquito life history was fit to time series data on the abundance of female An. funestus s.s. collected over 2 years in southern Tanzania. Prior values of fitness and demography were incorporated from empirical data on larval development, adult survival and fecundity from laboratory-reared first generation progeny of wild caught An. funestus. The model was structured to allow larval and adult fitness traits to vary seasonally in response to environmental covariates (i.e. temperature and rainfall), and for density dependency in larvae. We measured the effects of density dependence and seasonality through counterfactual examination of model fit with or without these covariates.Results: The model accurately reconstructed the seasonal population dynamics of An. funestus and generated biologically-plausible values of their survival larval, development and fecundity in the wild. This model suggests that An-funestus survival and fecundity annual pattern was highly variable across the year, but did not show consistent seasonal trends either rainfall or temperature. While the model fit was somewhat improved by inclusion of density dependence, this was a relatively minor effect and suggests that this process is not as important for An. funestus as it is for An. gambiae populations.Conclusion: The model's ability to accurately reconstruct the dynamics and demography of An. funestus could potentially be useful in simulating the response of these populations to vector control techniques deployed separately or in combination. The observed and simulated dynamics also suggests that An. funestus could be playing a role in year-round malaria transmission, with any apparent seasonality attributed to other vector species.
Integrative Genomic Analysis of Drug Resistance in MET Exon 14 Skipping Lung Cancer Using Patient-Derived Xenograft Models
Authors: Xu Yunhua , Gu Linping , Li Yingqi , Zhao Ruiying , Jian Hong , Xie Wenhui , Liu Liu , Wu Huiwen , Han Yuchen , Lu Shun 
Published: January 2022

Abstract
Background
Non-small cell lung cancer (NSCLC) driven by MET exon 14 skipping (METex14) occurs in 3-4% of NSCLC cases and defines a subset of patients with distinct characteristics. MET targeted therapy has led to strong clinical responses, however little is known about aquired resistance to drugs in these patients. Patient derived xenograft (PDX) models are recognized as excellent preclinical models to facilitate the understanding of the mechanisms underlying drug resistance.
Methods
We describe a patient case harboring METex14 who exhibited drug resistance after treatment with crizotinib. Subcutaneous xenografts were generated from pretreatment and post-resistance patient specimens. PDX mice were then treated with MET inhibitors (crizotinib and tepotinib) to evaluate their drug response. DNA and RNA sequencing analysis was performed on patient tumor specimens and matching xenografts.
Results
PDXs preserved most of the histological and molecular profiles of the parental tumors. Drug resistance to MET targeted therapy was confirmed in PDX models through in vivo drug analysis. Newly aquired MET D1228N mutations and EGFR amplificated were detected in patient-resistant tumor specimens. Although the mutations were not detected in the PDX, EGFR overexpression was observed in RNA sequencing analysis indicating possible off target resistance through the EGFR bypass signaling pathway.
Conclusions
We established and characterized a pair of METex14 NSCLC patient-derived xenografts (PDXs), including the first crizotinib resistant METex14 PDX. This model will be a powerful tool for testing hypotheses of drug resistance mechanisms and investigations into novel therapeutic strategies.
Long Non-Coding RNA HAGLROS Promotes the Malignant Progression of Bladder Cancer by Regulating the miR-330-5p/SPRR1B Axis
Authors: Xiao Shiwei , Zuo Yigang , Li Yanan , Huang Yinglong , Fu Shi , Yuan Dongbo , Qiao Xuhua , Wang Haifeng , Wang Jiansong 
Published: January 2022

Abstract
BackgroundBladder cancer (BC) is the most common genitourinary malignancy worldwide, and its aetiology and pathogenesis remain unclear. Long noncoding RNAs can play vital roles in gene expression and diverse biological processes, especially in cancers. Accumulating evidence has shown that HAGLROS, a novel lncRNA, is closely related to the occurrence and progression of various cancers. However, the biological functions and underlying mechanisms of HAGLROS in BC remain unknown.MethodsThe relative expression of HAGLROS in BC was determined by bioinformatics analysis, transcriptome sequencing analysis and qRT–PCR. Gain- or loss-of-function assays were performed to study the biological roles of HAGLROS in BC. A CCK-8 assay was used to detect BC cell proliferation. BC cell invasion and migration were investigated by wound healing and Transwell assays. The cell cycle was analysed by flow cytometry assay. Western blot analysis and immunohistochemistry were performed to evaluate SPRR1B expression. The differential expression of candidate genes and their relationships were evaluated in data retrieved from the starBase database, the GEIPIA database, the Lnc2Cancer database and the LncBase database. FISH assays, subcellular fractionation assays and luciferase reporter assays were performed to explore the underlying molecular mechanisms of HAGLROS.ResultsHAGLROS expression is significantly upregulated in BC tissues and cells, and increasing HAGLROS expression was related to high pathologic grade. HAGLROS enhances the proliferation, migration and invasion of BC. Furthermore, SPRR1B is obviously upregulated and miR-330-5p is significantly downregulated in BC. Mechanistically, we found that HAGLROS is mainly located in the cytoplasm and positively regulates SPRR1B expression by sponging miR-330-5p, playing an oncogenic role in BC pathogenesis.ConclusionsThe present study demonstrates that HAGLROS is significantly overexpressed and plays an oncogenic role by regulating the miR-330-5p/SPRR1B axis in BC. HAGLROS may serve as a potential biomarker for the diagnosis and treatment of BC.