bluuebunny/crossref_metadata_2025_split huggingface dataset:
Living beyond Placenta Accreta Spectrum: Mother’s and Father’s Experience of the Postnatal Journey and Recommendations for an Integrated Care Pathway
Authors: Bartels Helena C , Horsch Antje , Cooney Naomi , Brennan Donal J , Lalor Joan G 
Published: March 2022

Abstract
Background
Placenta Accreta Spectrum is associated with significant clinical maternal morbidity and mortality, which has been extensively described in the literature. However, there is a dearth of research on the lived experiences of couples. The aim of this study is to describe living beyond a pregnancy and birth complicated by PAS for up to four years postpartum. Couples’ experiences of PAS inform the development of an integrated care pathway of family centered support interventions.
Methods
An Interpretative Phenomenological Analysis approach was applied to collect data through virtual interviews over a 3-month period from February to April 2021. Twenty-nine participants shared their stories; 6 couples were interviewed together (n = 12 participants), 6 couples were interviewed separately (n = 12 participants), and 5 women were interviewed without their partner. Couples were eligible for inclusion if they had a diagnosis of PAS within the previous 5 years. This paper focuses on the postnatal period, with data from the antenatal and intrapartum periods described separately.
Results
One superordinate theme “Living beyond PAS” emerged from interviews, with 6 subordinate themes as follows; “Living with a different body”, “The impact on relationships”, “Coping strategies”, “Post-traumatic growth”, “Challenges with normal care” and recommendations for “What needs to change”. These themes informed the development of an integrated care pathway for couples to support them from diagnosis up to one year following the birth.
Conclusion
Couples described the challenges of the postnatal period in terms of the physical and emotional impact, and how some were able to make positive life changes in the aftermath of a traumatic event. An integrated care pathway of simple supportive interventions, based on participant recommendations, delivered as part of specialist multidisciplinary team care may assist couples in alleviating some of these challenges.
Salivary Cell-Free HSD17B1 and HSPA1A Transcripts as Potential Biomarkers for Estrus Identification in Buffaloes (Bubalus Bubalis)
Authors: Singha Shubham , Baithalu Rubina K. , Pandey Mamta , Jaiswal Latika , Dash Sangram , Fernandes Abhijeet , Mohanty Tushar K. , Kumaresan A. , Maharana Biswa Ranjan , Sarath T. 
Published: March 2022

Abstract
Background: Estrus detection is a major problem in buffaloes because of the poor expression of estrus signs leading to low reproductive efficiency. Salivary transcripts analysis is a promising tool to identify biomarker; therefore, the present study was carried out to evaluate their potential as estrus biomarker. Methods: The levels of HSD17B1, INHBA, HSPA1A, Testin transcripts were compared in saliva during estrous cycle stages [early proestrus (day -2, EP), late proestrus (day-1, LP), estrus (E), metestrus (ME) and diestrus (DE)] of cyclic heifers (n=8) and pluriparoous (n=8) buffaloes by employing quantitative real-time polymerase chain reaction (qRT-PCR). Results: The levels of HSD17B1 (EP/DE 1.46-2.43 fold, LP/DE 2.49-3.06 fold; E/DE 7.21-11.9-fold P<0.01; ME/D 1.0-1.16 fold) and HSPA1A (EP/DE 0.93-2.39 fold, LP/DE 2.68-3.23 fold; E/DE 8.52-15.18 fold P<0.01; ME/D 0.86-1.01 fold) were significantly altered during the estrus than other estrous cycle stages in both cyclic heifers and pluriparous buffaloes. Receiver operating characteristic curve analysis revealed ability of salivary HSD17B1 (AUC 0.96; P<0.001) and HSPA1A (AUC 0.99; P<0.01) to differentiate E from other stages of estrous cycle. Conclusion: Significantly higher levels of HSD17B1 and HSPA1A transcripts in saliva during estrus phase suggest their biomarkers potential for estrus detection in buffaloes.
Theoretical study of the chiral separation process of atenolol and carvedilol β-blocker drugs
Authors: Maia Pollyanna P , Nascimento Camila A , Silva Camilla F , Nascimento Clebio Soares 
Published: March 2022

Abstract
In the present work, we perform a theoretical study to understand the chiral recognition process of polysaccharide-derived stationary phases with two β-blocker drugs: atenolol and carvedilol. According to the main results, the structural and thermodynamic parameters obtained by DFT calculations pointed out the chiral discrimination, as well as the enantiomeric elution order of both β-blockers, thus explaining at the molecular level the HPLC experimental data previously obtained. Finally, the relative stability of distinct diastereoisomeric complexes was explained by the hydrogen bonds and π-π stacking interactions formed between β-blockers enantiomers and their respective chiral selectors.
Anesthesia personnel’s visual attention regarding patient monitoring in simulated routine and critical incidents. An eye-tracking study.
Authors: Roche Tadzio R. , Maas Elise J. , Said Sadiq , Braun Julia , Machado Carl , Spahn Donat R. , Noethiger Christoph B. , Tscholl David W. 
Published: March 2022

Abstract
Background: Cognitive ergonomics design of patient monitoring may reduce human factor errors in high-stress environments. Eye-tracking is a suitable tool to gain insight into the visual interaction of healthcare professionals with patient monitors, which may facilitate their further development.Methods: This prospective, randomized, high-fidelity simulation study compared anesthesia personnel's visual attention (fixation count and dwell time) to 15 areas of interest on the patient monitor during critical and non-critical anesthesia scenarios. Furthermore, we examined the extent to which participants' experience influenced visual attention and which vital signs displayed on the patient monitor received the most visual attention. We used mixed zero-inflated Poisson regression and mixed linear models to analyze the results.Results: Analyzing 23 ten-minute scenarios, we found significantly more fixations to the areas of interest on the patient monitor during critical situations (rate ratio of 1.45; 95% CI 1.33 to 1.59; p < 0.001). However, the dwell-time on the areas of interest did not significantly differ between the critical and non-critical scenarios (coefficient of –1.667; 95% CI –4.549 to 1.229; p = 0.27). The professional experience did not significantly influence the visual attention (fixation: rate ratio of 0.88; 95% CI 0.54 to 1.43; p = 0.61 and dwell-time: coefficient of 0.889; 95% CI –1.465 to 3.229; p = 0.27). Regarding vital signs, anesthesia personnel paid the most attention to blood pressure (fixation: mean [SD] of 108 [74.83]; dwell-time: mean [SD] of 27 [15.90] seconds), end-expiratory carbon dioxide (fixation: mean [SD] of 59 [47.39]; dwell-time: mean [SD] of 30 [21.51] seconds), and the electrocardiogram (fixation: mean [SD] of 58 [64.70]; dwell-time: mean [SD] of 15 [14.95] seconds).Conclusions: Critical anesthesia situations increased anesthesia personnel’s visual interaction with the patient monitor. Furthermore, we found that their visual attention focused mainly on a few vital signs. To assist clinicians in critical situations, manufacturers should optimize monitors to convey necessary information as easily and quickly as possible and optimize the visibility of less frequently observed but equally critical vital signs, especially when they are in an abnormal range.
Design and validation of a Theory-Based Physical Activity Assessment Questionnaire for the Pregnant Women (PA2Q-PW)
Authors: Kianfard Leila , Niknami Shamsaddin , Shokravi Farkhonde Amin , Rakhshanderou Sakineh 
Published: March 2022

Abstract
Background: There is evidence that physical activity plays an essential role in preventing illnesses during pregnancy. This study aimed to design and validation a theory-based physical activity assessment questionnaire for pregnant women (PA2Q-PW). Methods: In this exploratory psychometric study, a physical activity assessment questionnaire for Pregnant Women (PA2Q-PW) was designed. Face-to-face interviews were conducted to develop the initial items of PA2Q-PW. The findings from interviews were merged with the literature review. The face, construct, and content validity of PA2Q-PW was assessed by the participation of pregnant women, midwives, and health educationists. Impact Score (IS), Content Validity Index (CVI), and Content Validity Ratio (CVR) and of the items were calculated. Construct validity of PA2Q-PW was calculated through factor analysis. Intra-class correlation coefficient (ICC), Cronbach’s alpha, standard error of measurement (SEM), and composite reliability were calculated to assess the reliability of the findings by PA2Q-PW.Results: The highest score was seen in the Knowledge subscale of PA2Q-PW, and the value of Cronbach's alpha for the subscales was from 0.89 to 0.97, and the value of ICC was from 0.76 to 0.89. The correlation (CVI & CVR indices) between the experts was satisfactory in all items of PA2Q-PW. The minimum loading factor for varimax rotation in the knowledge subscale was 0.41, in attitude was 0.56, in nurtures was 0.38, and in enabling factor was 0.27. The fitness of the proposed model among those constructs was confirmed by Root Mean Square Error of Approximation (RMSEA) <0.08, Normed chi-square (x 2/ df) < 5.0, Comparative Fit Index (CFI) ≥ 0.90, Tucker- Lewis Index (TLI) ≥ 0.9.Conclusion: PA2Q-PW is the first standard tool based on the PEN-3 cultural model that researchers can utilize to gather data and conduct the desired education interventions to change physical activity behavior in pregnant women during pregnancy. Trial ID: ISRCTN13356179
Hydrological modelling to assess the impacts of socio-economic development and climate change on water resources in Cauvery Basin, India
Authors: Gupta Bhawana , Horan Robyn , Rowan John , Allan Andrew 
Published: March 2022

Abstract
In order to address the sustainability challenges emerging from interdependencies in water, energy, food nexus, in recent years ecosystem services have been identified as an important link in the nexus. Ecosystem services are essential for environmental sustainability and for provision of natural resources in WEF sectors and its degradation due to climate change and socio-economic development impacts resource security in all sectors creating a feedback loop of exploitation. This study highlights the need to integrate freshwater ecosystem health in planning development strategies as sustainable freshwater ecosystem is vital for climate change resilient future. Integrated hydrological models have been widely used to identify scenarios with optimal economic benefits in agriculture, energy and urban sectors from a water development project, in this research we have used GWAVA model to assess the impacts of changes in water use efficiency, land use change, population growth and climate change on water resources and ecologically relevant hydrological regime indicators at a basin scale. The analysis of model stimulated stream flows was used to compare the sensitivity of water resource availability induced by natural or anthropogenic changes at a sub-catchment scale. The results suggest significant uncertainties in availability and use of river water resources in the mid-century, however, aligned with the global and national climate change commitments, results shows that demand side management of water and sustainable land use management can mitigate immediate consequences of climate change on river health.
Parallel Exploratory And Confirmatory Factor Analysis Of The Hungarian Fear Of Covid-19 Scale In A Large General Population Sample: A Psychometric And Dimensionality Evaluation
Authors: Balázs Péter György , Ariel Mitev , Brodszky Valentin 
Published: March 2022

Abstract
Background: The seven-item Fear of Covid-19 Scale (FCV-19S) is the most frequently applied instruments for assessing fear of COVID-19 infection. Our objectives were to develop a Hungarian version of FCV-19S, to evaluate its psychometric properties and dimensionality.Methods: In May 2021, a sample of adults representative of the Hungarian general population completed an online questionnaire survey with respect of the COVID-19 pandemic. Outcome measures included FCV-19S, General Anxiety Disorder (GAD-7) questionnaire and Patient Health Questionnaire (PHQ-9) depressive severity score. Item characteristics, validity and internal consistency reliability of FCV-19S were determined. Maximum likelihood method EFA was used to measure item-factor relationships (h²; factor loading; eigenvalues), while goodness-of-fit of the FCV-19 was evaluated by comparison of one and two-factor CFA model fits (RMSEA, SRMR, χ²/df, CFI, NFI, RFI, IFI, TLI). Correlations explored the relationships between fear, anxiety and depression and FCV-19 scores were compared in vaccinated and non-vaccinated subgroups.Results: Overall, n=2000 responded the online questionnaire. The mean age was 49.1, majority being females (62.2%). EFA identified a maximum of two factors (eig=4.2 and 1.01). Items 3,4,6,7 strongly correlated with Factor 1 (physiological fear) and items 1,2,5 linked to Factor 2 (emotional fear). FCV-19 items showed good internal consistency (Cronbach α=0.88) and correlated moderately or highly, while showing weak to moderate correlation with PHQ-9 (r=0.364) and GAD-7 (r=0.424). Significant differences were observed between vaccinated (14.2) and non-vaccinated (13.1) FCV-19 mean scores.Conclusions: The Hungarian version of the FCV-19 Scale seems valid and reliable construct to measure fear associated with COVID-19. Both uni- and bi-dimensional CFA is feasible, though the two-factor structure faces some limitations.
Estimation of Model Accuracy By A Unique Set of Features And Tree-Based Regressor
Authors: Bitton Mor , Keasar Chen 
Published: March 2022

Abstract
Computationally generated models of protein structures bridge the gap between the practically negligible price tag of sequencing and the high cost of experimental structure determination. By providing a low-cost (often free) partial alternative to experimentally determined structures, these models help biologists design and interpret their experiments. Obviously, the more accurate the models the more useful they are. However, methods for protein structure prediction generate many structural models (called decoys) of various qualities, necessitating means for the estimation of their accuracy. Here we present MESHI_consensus, a new method for the estimation of model accuracy. The method uses a tree based regressor and a set of structural, target-based, and consensus-based features. The new method achieved high performance in the EMA (Estimation of Model Accuracy) track of the recent CASP14 community-wide experiment (https://predictioncenter.org/casp14/index.cgi). The tertiary structure prediction track of that experiment revealed an unprecedented leap in prediction performance by a single prediction group/method, namely AlphaFold2. This achievement would inevitably have a profound impact on the field of protein structure prediction, including the accuracy estimation sub-task. We conclude this manuscript with some speculations regarding the future role of accuracy estimation in a new era of accurate protein structure prediction
The Genome of Rice Planthopper Egg Parasitoid Wasps Anagrus Nilaparvatae (Hymenoptera: Mymaridae) Casts Light on the Chemo- and Mechano-Sensing in Parasitism
Authors: Ma Ying , Guo Zixiao , Wang Liyang , Wang Bingyang , Huang Tingfa , Tang Bingjie , Zhang Guren , Zhou Qiang 
Published: March 2022

Abstract
Background
Mymaridae is an ancient insect group, which is a basal lineage in the Chalcidoidea phylogeny. The species of Mymaridae have great potential for biological control. Anagrus nilaparvatae, a representative specie of Mymaridae, is ideal for controlling rice planthopper due to its high rate of parasitism by finding hosts efficiently from paddy ridges to fields.
Results
Using both single-molecule real-time and Illumina sequencing, we sequenced and assembled the whole genome of A. nilaparvatae, which is the first genome sequenced in family Mymaridae. The assembly consists of 394 scaffolds accumulated to 488.8Mb. The assembly is of high continuity and completeness, indicated by the N50 value of 25,368,259bp and 98.4% mapping rate of BUSCO orthologs. 16894 protein-coding genes in the genome were annotated. The phylogenomic tree constructed for A. nilaparvatae and other 12 species of Hymenoptera confirmed that family Mymaridae is basal in the Chalcidoidea superfamily and sister to all the remaining Chalcidoidea species. The divergence between A. nilaparvatae and the other seven Chalcidoidea species was dated at ~ 126.9 Mya. Chemoreceptor and mechanoreceptor genes are important in the parasitic behavior. We identified 17 OBPs, 11 CSPs, 4 NPC2s, 88 ORs, 7 GRs, 22 IRs and 13 SNMPs in the genome of A. nilaparvatae, which are associated with the chemosensory function. Strikingly, there are only 1 PPK and 3 TRP genes in the genome that are relevant to the mechanosensory function.
Conclusions
The small numbers of chemo- and mechano-sensory genes in A. nilaparvatae indicate the species-specific host detection and oviposition behavior of A. nilaparvatae might be regulated by relatively simple molecular pathways.
Social skills in neurodevelopmental disorders: a study using role-plays to assess adolescents and young adults with 22q11.2 deletion syndrome and autism spectrum disorders
Authors: Feller Clémence Mathilde , Ilen Laura , Eliez Stephan , Schneider Maude 
Published: March 2022

Abstract
Backgrounds: Social skills are frequently impaired in neurodevelopmental disorders, including 22q11.2 deletion syndrome (22q11DS) and autism spectrum disorders (ASD). Although often assessed with questionnaires, direct assessment provides a more valid estimate of the constructs. Role-plays (i.e., simulates situational settings) therefore appear to be an appropriate indicator of social skills in daily life, as they allow for systematic and direct observations of behaviors. Methods: 53 individuals with 22q11DS, 34 individuals with ASD, and 64 typically developing (TD) peers aged 12-30 years were assessed with role-plays as well as hetero-reported questionnaires and clinical interviews on social skills, functioning and anxiety. Results: Both clinical groups showed impaired social skills compared to TD, but distinct social profiles emerged between the groups. Individuals with ASD exhibited high social inadequacy, whereas individuals with 22q11DS were characterized by a lack of assertiveness. No association was found between social skills measured by direct observation and caregiver reports. Social anxiety, although greater in clinical groups than in TD, was not associated with social skills. Conclusions: This study highlights the need to train social skills through tailored interventions to target the specific difficulties of each clinical population. It also highlights the importance of combining measures as they do not necessarily provide the same outcome.
Efficacy of ultrasound endoscopy with artificial intelligence for the differential diagnosis of non-gastric gastrointestinal stromal tumors
Authors: Ihara Eikichi , Minoda Yosuke , Fujimori Nao , Nagatomo Shuzaburo , Esaki Mitsuru , Hata Yoshitaka , Bai Xiaopeng , Tanaka Yoshimasa , Ogino Haruei , Chinen Takatoshi , Hu Qingjiang , Oki Eiji , Yamamoto Hidetaka , Ogawa Yoshihiro 
Published: March 2022

Abstract
Gastrointestinal stromal tumors (GISTs) are common subepithelial lesions (SELs) and require treatment considering their malignant potential. We recently developed an endoscopic ultrasound-based artificial intelligence (EUS-AI) system to differentiate GISTs from non-GISTs in gastric SELs, which were used to train the system. We assessed whether the EUS-AI system designed for diagnosing gastric GISTs could be applied to non-gastric GISTs. Between January 2015 and January 2021, 52 patients with non-gastric SELs (esophagus, n = 15; duodenum, n = 26; colon, n = 11) were enrolled. The ability of EUS-AI to differentiate GISTs from non-GISTs in non-gastric SELs was examined. The accuracy, sensitivity, and specificity of EUS-AI for discriminating GISTs from non-GISTs in non-gastric SELs were 94.4%, 100%, and 86.1%, respectively, with an area under the curve of 0.98 based on the cutoff value set using the Youden index. In the subanalysis, the accuracy, sensitivity, and specificity of EUS-AI were highest in the esophagus (100%, 100%, 100%; duodenum, 96.2%, 100%, 0%; colon, 90.9%, 100%, 0%); the cutoff values were determined using the Youden index or the value determined using stomach cases. The diagnostic accuracy of EUS-AI increased as lesion size increased, regardless of lesion location. EUS-AI based on gastric SELs had good diagnostic ability for non-gastric GISTs.
The effect of the central administration of the Neuropeptide VF on feed intake and its possible interactions with glutamate and opioid systems in broiler chicken
Authors: Hamidi Behnam , Zendehdel Morteza , Vazir Bita , Asghari Ahmad 
Published: March 2022

Abstract
Arginine-phenylalanine-amide (RFamide)-related peptides (RFRP) known as neuropeptide VF (NPVF) have a crucial role in feeding regulation. The present study purposed to determine the effect of intracerebroventricular (ICV) injection of the NPVF on dietary intake and possible interactions of NPVF with glutamate and opioid systems in broiler chicken. In experiment 1, chickens received ICV administration of the control solution, NPVF (16 nmol), 15 nmol of MK-801 (NMDA glutamate receptors antagonist), and injection of the NPVF with MK-801 together. In experiments, 2-8 chicken injected intracerebroventricularly with 390 nmol of CNQX (antagonist of AMPA glutamate receptors), 2 nmol of AIDA (an antagonist of mGLUR1 glutamate receptors), 150 nmol of LY341495 (an antagonist of mGLUR2 glutamate receptors), 2 nmol of UBP1112 (an antagonist for mGLUR3 glutamate receptors), 5 µg of β-FNA (an antagonist for mu (µ) receptors), 5 µg of NTI (an antagonist for delta (δ) receptors), nor-BNI (kappa (κ) receptors antagonist; 5 µg) instead of MK-801. Following the injection, at 30, 60, and 120 min, the chick's dietary intake was recorded. As a result, NPVF (16 nmol) reduced feed intake in broilers (P<0.05). NPVF + MK-801 co-injection reduced the hypophagic action of the NPVF (P<0.05). Injection of NPVF + CNQX together reduced the anorectic action of NPVF (P<0.05). Co-injection of NPVF + β-FNA reduced effects of NPVF injection (P<0.05). Thus, in newborn broilers, NPVF-induced anorexia is probably mediated by NMDA/ AMPA glutamate and µ opioid receptors.