bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation on pore structure regulation of activated carbon derived from sargassum and its application in supercapacitor
Authors: Li Shijie , Tan Xiaopeng , Li Hui , Gao Yan , Wang Qian , Li Guoning , Guo Min 
Published: June 2022

AbstractIn order to realize the effective regulation of the pore structure of activated carbon and optimize its pore structure properties as electrode material, the effects of activation temperature, activation time and impregnation ratio on the specific surface area, total pore volume and average pore diameter of activated carbon prepared by sargassum are studied by orthogonal experiment. In addition, the electrochemical properties of sargassum-based activated carbon (SAC) and the relationship between the gravimetric capacitance and specific surface area of SAC are also studied. The SACs prepared under all conditions have high specific surface area (≥ 2227 m2 g−1) and developed pore structure, in which the pore diameter of micropores mainly concentrated in 0.4 ~ 0.8 nm, the pore diameter of mesopores mainly concentrated in 3 ~ 4 nm, and the number of micropores is far more than that of mesopores. In the activation process, the impregnation ratio has the greatest effect on the specific surface area of SAC, the activation temperature and impregnation ratio have significant effect on the total pore volume of SAC, and the regulation of the average pore diameter of SAC is mainly realized by adjusting the activation temperature. The SACs exhibit typical electric double layer capacitance performances on supercapacitors, delivering superior gravimetric capacitance of 237.3 F g−1in 6 mol L−1KOH electrolyte system at current density of 0.5 A g−1and excellent cycling stability of capacitance retention of 92% after 10,000 cycles. A good linear relationship between gravimetric capacitance and specific surface area of SAC is observed.
Patient and economic impact of implementing a paediatric sepsis pathway in emergency departments in Queensland, Australia
Authors: Blythe Robin , Lister Paula , Seaton Robert , Harley Amanda , Schlapbach Luregn J. , McPhail Steven , Venkatesh Bala , Irwin Adam , Raman Sainath ,   the Queensland State-wide Sepsis Collaborative, Schlapbach Luregn , Harley Amanda , Irwin Adam , Graham Nicolette , Thomson Fiona , Owen Kieran , Garrish Kirsten , Sampson Emma , Long Debbie , George Shane , Grimwood Keith , Bell Christa , Semple Bethany , Adams Claire , Brown Josea , Maloney Louise , Lister Paula , Schofield Scott , Thomas Clare , Dejong Liam , Bently Esther , Halkidis Lambros , Bird Cheryl , Smith Matthew , Alexander Pia , Davidson-West Laura , Adegbija Titiosibina Ebenezer , Brandt Alice , Walker Bree , McLucas Andrea , Michael Adam Philip , Hoole Samantha , Bauer Candice , Sutherland John , Thomas Douglas Gordon , Van der Walt David , Hulme Jessica , Burke Kerrie , Cooney Helena , Morel Doug , O’Riordan Louise , Fairless Samantha , Bool Megan , Choudary Nandini , Arora Shalini , Lawton Ben , Farrell Jo , Prasad Penelope , Prasad Rudesh , O’Connor Laura , Butters Timothy , Kennedy Peter , Pham Hanh , Aoude Maya , Blundell Sara , Willmett Natasha , McGrath Louise , Smith Karen , Weller Kate , Maturanec Trina , Rice Michael , Venkatesh Balasubramanian , Lane Paul , Seaton Robert , Mason Donna , Mehta Naitik , Kalke Vikrant , Jones Damien , Ames Mathew , Steele Mary , Wilkinson Amy , Gibbons Kristen , Gilholm Patricia , Ergetu Endrias , Treadwell Rachel , Van Raders Tahlia , Minogue Jessicah 
Published: June 2022

AbstractWe examined systems-level costs before and after the implementation of an emergency department paediatric sepsis screening, recognition and treatment pathway. Aggregated hospital admissions for all children aged < 18y with a diagnosis code of sepsis upon admission in Queensland, Australia were compared for 16 participating and 32 non-participating hospitals before and after pathway implementation. Monte Carlo simulation was used to generate uncertainty intervals. Policy impacts were estimated using difference-in-difference analysis comparing observed and expected results. We compared 1055 patient episodes before (77.6% in-pathway) and 1504 after (80.5% in-pathway) implementation. Reductions were likely for non-intensive length of stay (− 20.8 h [− 36.1, − 8.0]) but not intensive care (–9.4 h [− 24.4, 5.0]). Non-pathway utilisation was likely unchanged for interhospital transfers (+ 3.2% [− 5.0%, 11.4%]), non-intensive (− 4.5 h [− 19.0, 9.8]) and intensive (+ 7.7 h, [− 20.9, 37.7]) care length of stay. After difference-in-difference adjustment, estimated savings were 596 [277, 942] non-intensive and 172 [148, 222] intensive care days. The program was cost-saving in 63.4% of simulations, with a mean value of $97,019 [− $857,273, $1,654,925] over 24 months. A paediatric sepsis pathway in Queensland emergency departments was associated with potential reductions in hospital utilisation and costs.
Salivary, serological, and cellular immune response to the CoronaVac vaccine in health care workers with or without previous COVID-19
Authors: Ortega Marina Mazzilli , da Silva Laís Teodoro , Candido Érika Donizetti , Zheng Yingying , Tiyo Bruna Tiaki , Ferreira Arthur Eduardo Fernandes , Corrêa-Silva Simone , Scagion Guilherme Pereira , Leal Fabyano Bruno , Chalup Vanessa Nascimento , Valério Camila Araújo , Schmitz Gabriela Justamante Händel , Ceneviva Carina , Corá Aline Pivetta , de Almeida Alexandre , Durigon Edison Luiz , Oliveira Danielle Bruna Leal , Palmeira Patricia , da Silva Duarte Alberto José , Carneiro-Sampaio Magda , Oshiro Telma Miyuki 
Published: June 2022

AbstractWe investigated the anti-SARS-CoV-2 post-vaccine response through serum and salivary antibodies, serum antibody neutralizing activity and cellular immune response in samples from health care workers who were immunized with two doses of an inactivated virus-based vaccine (CoronaVac) who had or did not have COVID-19 previously. IgA and IgG antibodies directed at the spike protein were analysed in samples of saliva and/or serum by ELISA and/or chemiluminescence assays; the neutralizing activity of serum antibodies against reference strain B, Gamma and Delta SARS-CoV-2 variants were evaluated using a virus neutralization test and SARS-CoV-2 reactive interferon-gamma T-cell were analysed by flow cytometry. CoronaVac was able to induce serum and salivary IgG anti-spike antibodies and IFN-γ producing T cells in most individuals who had recovered from COVID-19 and/or were vaccinated. Virus neutralizing activity was observed against the ancestral strain, with a reduced response against the variants. Vaccinated individuals who had previous COVID-19 presented higher responses than vaccinated individuals for all variables analysed. Our study provides evidence that the CoronaVac vaccine was able to induce the production of specific serum and saliva antibodies, serum virus neutralizing activity and cellular immune response, which were increased in previously COVID-19-infected individuals compared to uninfected individuals.
Long non-coding RNA HOTAIR regulates cytoskeleton remodeling and lipid storage capacity during adipogenesis
Authors: Potolitsyna Evdokiia , Hazell Pickering Sarah , Germier Thomas , Collas Philippe , Briand Nolwenn 
Published: June 2022

AbstractThe long non-coding RNA HOTAIR is the most differentially expressed gene between upper- and lower-body adipose tissue, yet its functional significance in adipogenesis is unclear. We report that HOTAIR expression is transiently induced during early adipogenic differentiation of gluteofemoral adipose progenitors and repressed in mature adipocytes. Upon adipogenic commitment, HOTAIR regulates protein synthesis pathways and cytoskeleton remodeling with a later impact on mature adipocyte lipid storage capacity. Our results support novel and important functions of HOTAIR in the physiological context of adipogenesis.
Low transcriptomic of PTPRCv1 and CD3E is an independent predictor of mortality in HIV and tuberculosis co-infected patient
Authors: Gebremicael Gebremedhin , Gebreegziabxier Atsbeha , Kassa Desta 
Published: June 2022

AbstractA comprehensive assessment of immunological profiles during HIV-TB co-infection is essential to predict mortality, and facilitate the development of effective diagnostic assays, therapeutic agents, and vaccines. Expression levels of 105 immune-related genes were measured at enrolment and 6th month follow-up from 9 deceased HIV and TB coinfected patients who died between 3 and 7th months follow-up and at enrolment, 6th and 18th month from 18 survived matched controls groups for 2 years. Focused gene expression profiling was assessed from peripheral whole blood using a dual-color Reverse-Transcription Multiplex Ligation-dependent Probe Amplification assay. Eleven of the 105 selected genes were differentially expressed between deceased individuals and survivor-matched controls at baseline. At baseline, IL4δ2 was significantly more highly expressed in the deceased group than survivor matched controls, whereas CD3E, IL7R, PTPRCv1, CCL4, GNLY, BCL2, CCL5, NOD1, TLR3, and NLRP13 had significantly lower expression levels in the deceased group compared to survivor matched controls. At baseline, a non-parametric receiver operator characteristic curve was conducted to determine the prediction of mortality of single genes identified CCL5, PTPRCv1, CD3E, and IL7R with Area under the Curve of 0.86, 0.86, 0.86, and 0.85 respectively. The expression of these genes in the survived control was increased at the end of TB treatment from that at baseline, while decreased in the deceased group. The expression of PTPRCv1, CD3E, CCL5, and IL7R host genes in peripheral blood of patients with TB-HIV coinfected can potentially be used as a predictor of mortality in the Ethiopian setting. Anti-TB treatment might be less likely to restore gene expression in the level expression of the deceased group. Therefore, other new therapeutics that can restore these genes (PTPRCv1, CD3E, IL7R, and CCL5) in the deceased groups at baseline might be needed to save lives.
Stria medullaris innervation follows the transcriptomic division of the habenula
Authors: Juárez-Leal Iris , Carretero-Rodríguez Estefanía , Almagro-García Francisca , Martínez Salvador , Echevarría Diego , Puelles Eduardo 
Published: June 2022

AbstractThe habenula is a complex neuronal population integrated in a pivotal functional position into the vertebrate limbic system. Its main afference is the stria medullaris and its main efference the fasciculus retroflexus. This neuronal complex is composed by two main components, the medial and lateral habenula. Transcriptomic and single cell RNAseq studies have unveiled the morphological complexity of both components. The aim of our work was to analyze the relation between the origin of the axonal fibers and their final distribution in the habenula. We analyzed 754 tracing experiments from Mouse Brain Connectivity Atlas, Allen Brain Map databases, and selected 12 neuronal populations projecting into the habenular territory. Our analysis demonstrated that the projections into the medial habenula discriminate between the different subnuclei and are generally originated in the septal territory. The innervation of the lateral habenula displayed instead a less restricted distribution from preoptic, terminal hypothalamic and peduncular nuclei. Only the lateral oval subnucleus of the lateral habenula presented a specific innervation from the dorsal entopeduncular nucleus. Our results unveiled the necessity of novel sorts of behavioral experiments to dissect the different functions associated with the habenular complex and their correlation with the distinct neuronal populations that generate them.
Image improvement of temporal focusing multiphoton microscopy via superior spatial modulation excitation and Hilbert–Huang transform decomposition
Authors: Hu Yvonne Yuling , Lin Chun-Yu , Chang Chia-Yuan , Lo Yuan-Long , Chen Shean-Jen 
Published: June 2022

AbstractTemporal focusing-based multiphoton excitation microscopy (TFMPEM) just provides the advantage of widefield optical sectioning ability with axial resolution of several micrometers. However, under the plane excitation, the photons emitted from the molecules in turbid tissues undergo scattering, resulting in complicated background noise and an impaired widefield image quality. Accordingly, this study constructs a general and comprehensive numerical model of TFMPEM utilizing Fourier optics and performs simulations to determine the superior spatial frequency and orientation of the structured pattern which maximize the axial excitation confinement. It is shown experimentally that the optimized pattern minimizes the intensity of the out-of-focus signal, and hence improves the quality of the image reconstructed using the Hilbert transform (HT). However, the square-like reflection components on digital micromirror device leads to pattern residuals in the demodulated image when applying high spatial frequency of structured pattern. Accordingly, the HT is replaced with Hilbert–Huang transform (HHT) in order to sift out the low-frequency background noise and pattern residuals in the demodulation process. The experimental results obtained using a kidney tissue sample show that the HHT yields a significant improvement in the TFMPEM image quality.
Single step calcium sulfate scale removal at high temperature using tetrapotassium ethylenediaminetetraacetate with potassium carbonate
Authors: Murtaza Mobeen , Alarifi Sulaiman A. , Rasm Mohammed Yousef , Kamal Muhammad Shahzad , Mahmoud Mohamed , Al-Ajmi Mohammed 
Published: June 2022

AbstractCalcium sulfate (CaSO4) scale has been identified as one of the most common scales contributing to several serious operating problems in oil and gas wells and water injectors. Removing this scale is considered an economically feasible process in most cases as it enhances the productivity of wells and prevents potential severe equipment damage. In this study, a single-step method utilizing potassium carbonate and tetrapotassium ethylenediaminetetraacetate (K4-EDTA) at high temperature (200 °F) has been used to remove CaSO4 scale. The CaSO4 scale was converted to calcium carbonate (CaCO3) and potassium sulfate (K2SO4) using a conversion agent, potassium carbonate (K2CO3), at a high temperature (200 °F) and under various pH conditions. Various parameters were investigated to obtain a dissolver composition at which the optimum dissolution efficiency is achieved including the effect of dissolver pH, soaking time, the concentration of K4-EDTA, the concentration of potassium carbonate (K2CO3), temperature impact and agitation effect. Fourier transform infrared, X-ray crystallography, ion chromatography, stability tests and corrosion tests were carried out to test the end product of the process and showcase the stability of the dissolver at high temperature conditions. A reaction product (K2SO4) was obtained in most of the tests with different quantities and was soluble in both water and HCl. It was observed that the dissolver solution was effective at low pH (7) and resulted in a negligible amount of reaction product with 3 wt% CaSO4 dissolution. The 10.5-pH dissolver was effective in most of the cases and provided highest dissolution efficiency. The reaction product has been characterized and showed it is not corrosive. Both 7-pH and 10.5-pH dissolvers showed high stability at high temperature and minimum corrosion rates. The single step dissolution process showed its effectiveness and could potentially save significant pumping time if implemented in operation.
A semantic analysis-driven customer requirements mining method for product conceptual design
Authors: Wu Xuan-Yu , Hong Zhao-Xi , Feng Yi-Xiong , Li Ming-Dong , Lou Shan-He , Tan Jian-Rong 
Published: June 2022

AbstractPrecise customer requirements acquisition is the primary stage of product conceptual design, which plays a decisive role in product quality and innovation. However, existing customer requirements mining approaches pay attention to the offline or online customer comment feedback and there has been little quantitative analysis of customer requirements in the analogical reasoning environment. Latent and innovative customer requirements can be expressed by analogical inspiration distinctly. In response, this paper proposes a semantic analysis-driven customer requirements mining method for product conceptual design based on deep transfer learning and improved latent Dirichlet allocation (ILDA). Initially, an analogy-inspired verbal protocol analysis experiment is implemented to obtain detailed customer requirements descriptions of elevator. Then, full connection layers and a softmax layer are added to the output-end of Chinese bidirectional encoder representations from Transformers (BERT) pre-training language model. The above deep transfer model is utilized to realize the customer requirements classification among functional domain, behavioral domain and structural domain in the customer requirement descriptions of elevator by fine-tuning training. Moreover, the ILDA is adopted to mine the functional customer requirements that can represent customer intention maximally. Finally, an effective accuracy of customer requirements classification is acquired by using the BERT deep transfer model. Meanwhile, five kinds of customer requirements of elevator and corresponding keywords as well as their weight coefficients in the topic-word distribution are extracted. This work can provide a novel research perspective on customer requirements mining for product conceptual design through natural language processing.
Identification of HOX signatures contributing to oral cancer phenotype
Authors: Padam Kanaka Sai Ram , Morgan Richard , Hunter Keith , Chakrabarty Sanjiban , Kumar Naveena A. N. , Radhakrishnan Raghu 
Published: June 2022

AbstractThe role of evolutionarily conserved homeobox-containing HOX genes as transcriptional regulators in the developmental specification of organisms is well known. The contribution of HOX genes involvement in oral cancer phenotype has yet to be fully ascertained. TCGA-HNSC HTSeq-counts and clinical data were retrieved from the GDC portal for oral cavity neoplasms. GEO datasets (GSE72627, GSE30784, GSE37991) were accessed and analyzed using GEO2R. Differential HOX gene expression was profiled using the DESeq2 R package with a log2 fold change cut-off (− 1 and + 1) and Benjamini–Hochberg p-adjusted value at ≤ 0.01. Gene set over-representation analysis and semantic analysis associated with the disease ontology was performed using the ClusterProfiler R package, and pathway over-representation analysis was performed using IMPaLa. HOX protein interaction network was constructed using the Pathfind R package. HOX phenotype associations were performed using Mammalian Phenotype Ontology, Human Phenotype Ontology, PhenGenI associations, Jensen tissues, and OMIM entries. Drug connectivity mapping was carried out with Dr. Insight R package. HOXA2 was upregulated in oral dysplasia but silenced during tumor progression. Loss of HOXB2 expression was consistent in the potentially malignant oral lesions as well as in the primary tumor. HOXA7, HOXA10, HOXB7, HOXC6, HOXC10, HOXD10, and HOXD11 were consistently upregulated from premalignancy to malignancy and were notably associated with risk factors. Overrepresentation analysis suggested HOXA10 was involved in the transcriptional misregulation contributing to the oral cancer phenotype. HOX genes subnetwork analysis showed crucial interactions with cell cycle regulators, growth responsive elements, and proto-oncogenes. Phenotype associations specific to the oral region involving HOX genes provide intrinsic cues to tumor development. The 5′ HOX genes were aberrantly upregulated during oral carcinogenesis reflecting their posterior prevalence.
Dietary total, plant and animal protein intake in relation to metabolic health status in overweight and obese adolescents
Authors: Lotfi Keyhan , Mohammadi Sobhan , Mirzaei Saeideh , Asadi Ali , Akhlaghi Masoumeh , Saneei Parvane 
Published: June 2022

AbstractFew studies have investigated dietary total protein intake and its subtypes in relation to metabolic health status. We explored the relation between dietary total, plant and animal protein intake with metabolic health status in Iranian overweight/obese adolescents. Overweight/obese adolescents (n = 203) were selected for this cross-sectional study by multistage cluster random-sampling method. A validated food frequency questionnaire was used to evaluate dietary intakes. Total, plant and animal protein intake were considered as percentage of energy intake. Anthropometric indices, blood pressure, lipid and glycemic profiles were collected. Participants were classified as metabolically healthy obese (MHO) or unhealthy obese (MUO) based on International Diabetes Federation (IDF) and IDF/Homeostasis Model Assessment Insulin Resistance (HOMA-IR) definitions. Subjects had a mean age of 13.98 years, and 50.2% of them were girls. Based on IDF criteria, adolescents in the top tertile of total (OR = 0.32; 95% CI 0.13–0.77), plant (OR = 0.30; 95% CI 0.10–0.91), and animal (OR = 0.20; 95% CI 0.08–0.54) protein intake had lower odds of being MUO compared to the reference category. Considering IDF/HOMA-IR criteria, subjects in the highest tertile of total (OR = 0.31; 95% CI 0.12–0.79) and animal (OR = 0.17; 95% CI 0.06–0.49) protein intake were less likely to be MUO. However, no substantial association was observed with plant protein intake. Also, an inverse association was observed between each SD increase in total and animal protein with MUO odds. We found inverse association between total, plant and animal protein intake and chance of being MUO in adolescents. Further prospective studies are needed to confirm the findings.
Flexural behaviour of strengthened damaged steel beams using carbon fibre-reinforced polymer sheets
Authors: Hou Wenyu , Wang Lianguang , Shi Di 
Published: June 2022

AbstractThis paper presents the test and finite element analysis results of a study on the flexural behaviour of damaged steel beams strengthened with carbon fibre-reinforced polymer (CFRP) sheets. The test results showed that the yield load, ultimate load and elastic stiffness of the steel beams with 100% loss of tension flange were 68.3%, 73.8% and 13.5% greater than the yield load, ultimate load and elastic stiffness of the steel beam with 28% loss of the web after static loading. The yield load and elastic stiffness of the steel beam after overloading were 8.7% and 24.5% greater than the yield load and elastic stiffness of the steel beam without overloading. The damage level had a significant effect on the yield load, ultimate bearing capacity and elastic stiffness of the steel beams regardless of whether the steel beams were under static loading or overloading. The damaged steel beam could be repaired by CFRP sheets, the increased layers of CFRP sheets could improve the yield load, ultimate bearing capacity and elastic stiffness of the steel beams, and the strains of CFRP sheets would decrease because of overloading. The numerical analysis results showed that compared with the steel beam without overloading, the deflection and strains of the steel beam after overloading were much smaller. The yield load and the elastic stiffness increased with the increment of the overloading amplitude, and the increment of overloading number could reduce the yield load and elastic stiffness. All the yield loads of the steel beams after overloading were greater than the yield loads of the steel beams without overloading, but the ultimate bearing capacities were smaller.