bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beef tallow injection matrix for serial crystallography
Authors: Nam Ki Hyun 
Published: January 2022

AbstractSerial crystallography (SX) enables the visualization of the time-resolved molecular dynamics of macromolecular structures at room temperature while minimizing radiation damage. In SX experiments, the delivery of a large number of crystals into an X-ray interaction point in a serial and stable manner is key. Sample delivery using viscous medium maintains the stable injection stream at low flow rates, markedly reducing sample consumption compared with that of a liquid jet injector and is widely applied in SX experiments with low repetition rates. As the sample properties and experimental environment can affect the stability of the injection stream of a viscous medium, it is important to develop sample delivery media with various characteristics to optimize the experimental environment. In this study, a beef tallow injection matrix possessing a higher melting temperature than previously reported fat-based shortening and lard media was introduced as a sample delivery medium and applied to SX. Beef tallow was prepared by heat treating fats from cattle, followed by the removal of soluble impurities from the extract by phase separation. Beef tallow exhibited a very stable injection stream at room temperature and a flow rate of < 10 nL/min. The room-temperature structures of lysozyme and glucose isomerase embedded in beef tallow were successfully determined at 1.55 and 1.60 Å, respectively. The background scattering of beef tallow was higher than that of previously reported fat-based shortening and lard media but negligible for data processing. In conclusion, the beef tallow matrix can be employed for sample delivery in SX experiments conducted at temperatures exceeding room temperature.
Understanding the uneven spread of COVID-19 in the context of the global interconnected economy
Authors: Tsiotas Dimitrios , Tselios Vassilis 
Published: January 2022

AbstractThe worldwide spread of the COVID-19 pandemic is a complex and multivariate process differentiated across countries, and geographical distance is acceptable as a critical determinant of the uneven spreading. Although social connectivity is a defining condition for virus transmission, the network paradigm in the study of the COVID-19 spatio-temporal spread has not been used accordingly. Toward contributing to this demand, this paper uses network analysis to develop a multidimensional methodological framework for understanding the uneven (cross-country) spread of COVID-19 in the context of the globally interconnected economy. The globally interconnected system of tourism mobility is modeled as a complex network and studied within the context of a three-dimensional (3D) conceptual model composed of network connectivity, economic openness, and spatial impedance variables. The analysis reveals two main stages in the temporal spread of COVID-19, defined by the cutting-point of the 44th day from Wuhan. The first describes the outbreak in Asia and North America, the second stage in Europe, South America, and Africa, while the outbreak in Oceania intermediates. The analysis also illustrates that the average node degree exponentially decays as a function of COVID-19 emergence time. This finding implies that the highly connected nodes, in the Global Tourism Network (GTN), are disproportionally earlier infected by the pandemic than the other nodes. Moreover, countries with the same network centrality as China are early infected on average by COVID-19. The paper also finds that network interconnectedness, economic openness, and transport integration are critical determinants in the early global spread of the pandemic, and it reveals that the spatio-temporal patterns of the worldwide spreading of COVID-19 are more a matter of network interconnectivity than of spatial proximity.
Assessment of the relationship between labial gingival thickness and the underlying bone thickness in maxillary anterior teeth by two digital techniques
Authors: Wang Linhong , Ruan Yan , Chen Jianping , Luo Yunxiao , Yang Fan 
Published: January 2022

AbstractThis study aimed to noninvasively assess the relationship between the labial gingival thickness (GT) and the underlying bone thickness (BT) of maxillary anterior teeth by two digital techniques. A total of 30 periodontally healthy participants with 172 maxillary anterior teeth were enrolled. GT and BT were measured at 2, 4 and 6 mm apical to the cemento-enamel junction (CEJ) by two digital techniques: M1—cone-beam computed tomography (CBCT) and M2—digital intraoral scanning (DIS) combined with CBCT. The Pearson's correlation coefficient was calculated to determine the correlation between GT and BT. A significant negative correlation was identified between GT and BT at 2 mm apical to the CEJ for central incisors (CI), lateral incisors (LI), and canines (CA) both by M1 and M2, while a weak negative correlation at 4 mm apical to the CEJ was observed by M1 for CA. No significant correlation was found at other sites by both M1 and M2. The labial BT was < 1 mm in most cases (85% of CI; 97% of LI; and 90% of CA). Within the limitation of this study, it was concluded that GT and BT seemed to be negatively correlated at 2 mm apical to the CEJ. Therefore, caution is warranted when implant restoration at the esthetic area of the anterior teeth.
Null results for the steal-framing effect on out-group aggression
Authors: Mifune Nobuhiro 
Published: January 2022

AbstractWhether intergroup conflict is a necessary condition for the evolution of human prosociality has been a matter of debate. At the center of the debate is the coevolutionary model of parochial altruism—that human cooperation with in-group members has coevolved with aggression toward out-group members. Studies using the intergroup prisoner’s dilemma–maximizing difference game to test the model have repeatedly shown that people do not exhibit out-group aggression, possibly because of an inappropriate operationalization and framing of out-group aggression. The coevolutionary model predicts out-group aggression when the actor understands that it will lead to the in-group’s benefit. However, in the game, such an aspect of out-group aggression that benefits the in-group is typically not well communicated to participants. Thus, this study tested the hypothesis that out-group aggression in the game would be promoted by a framing that emphasizes that attacking out-group members enhances the in-group’s gain. Results of two laboratory experiments with 176 Japanese university students in total showed that such a framing did not promote out-group aggression and individuals invested more money to cooperate with in-group members only, avoiding the strategy of cooperating with in-group members to harm out-group members. These results do not support the coevolutionary model.
Specific gut bacterial responses to natural diets of tropical birds
Authors: Bodawatta Kasun H. , Klečková Irena , Klečka Jan , Pužejová Kateřina , Koane Bonny , Poulsen Michael , Jønsson Knud A. , Sam Katerina 
Published: January 2022

AbstractThe composition of gut bacterial communities is strongly influenced by the host diet in many animal taxa. For birds, the effect of diet on the microbiomes has been documented through diet manipulation studies. However, for wild birds, most studies have drawn on literature-based information to decipher the dietary effects, thereby, overlooking individual variation in dietary intake. Here we examine how naturally consumed diets influence the composition of the crop and cloacal microbiomes of twenty-one tropical bird species, using visual and metabarcoding-based identification of consumed diets and bacterial 16S rRNA microbiome sequencing. We show that diet intakes vary markedly between individuals of the same species and that literature-based dietary guilds grossly underestimate intraspecific diet variability. Furthermore, despite an effect of literature-based dietary guild assignment of host taxa, the composition of natural diets does not align with crop and cloacal microbiome similarity. However, host-taxon specific gut bacterial lineages are positively correlated with specific diet items, indicating that certain microbes associate with different diet components in specific avian hosts. Consequently, microbiome composition is not congruent with the overall consumed diet composition of species, but specific components of a consumed diet lead to host-specific effects on gut bacterial taxa.
A bioinspired, electroactive colorable and additive manufactured photonic artificial muscle
Authors: Ma Wentao , Li Bo , Jiang Lei , Sun Ya , Wu Yehui , Zhao Pengfei , Chen Guimin 
Published: January 2022

A structure-colorable flexural artificial muscle (FlexAM) is developed by integrating both a flexible two-dimensional grating pattern layer and dielectric elastomers via additive laminated object manufacturing. The FlexAM harnesses the view angle-dependence to deliver a voltage-activated and ultrafast coloration performance at a rate of 2.814 nm ms−1.
Insights from structural studies of the cardiovirus 2A protein
Authors: Caliskan Neva , Hill Chris H. 
Published: January 2022

Abstract
Cardioviruses are single-stranded RNA viruses of the family Picornaviridae. In addition to being the first example of internal ribosome entry site (IRES) utilization, cardioviruses also employ a series of alternative translation strategies, such as Stop-Go translation and programmed ribosome frameshifting. Here, we focus on cardiovirus 2A protein, which is not only a primary virulence factor, but also exerts crucial regulatory functions during translation, including activation of viral ribosome frameshifting and inhibition of host cap-dependent translation. Only recently, biochemical and structural studies have allowed us to close the gaps in our knowledge of how cardiovirus 2A is able to act in diverse translation-related processes as a novel RNA-binding protein. This review will summarize these findings, which ultimately may lead to the discovery of other RNA-mediated gene expression strategies across a broad range of RNA viruses.
Investigation of impact of DC component on breakdown characteristics for different electric fields under composite AC & DC voltage
Authors: Ispirli Mehmet Murat , Kalenderli Özcan , Seifert Florian , Rock Michael , Oral Bülent 
Published: January 2022

AbstractThe valve side of the converter in the high‐voltage direct current is subjected to mixed voltages such as composite AC & DC voltage. In this study, the effects of the homogeneity of electric field on breakdown voltage were investigated for different ±DC component amplitudes of the composite voltage. The field efficiency factor was calculated using mean and maximum field strengths for all of them. Variation of breakdown voltage of air was examined under the composite AC & DC voltage for different ratios ±DC. As one result of the study, the breakdown occurs at the positive half‐wave of the AC voltage despite −DC voltage being applied due to positive corona discharge pulses. This breakdown point is named as the polarity change point. The breakdown voltage increases with the decrease of DC voltage component up to polarity change point in non‐uniform electric field. In less uniform electric field, the AC breakdown voltage was measured slightly higher than the DC breakdown voltage. In less uniform electric field, as the ratio of the applied AC voltage to DC voltage increases, the breakdown voltage gradually approaches the AC breakdown voltage. This result is similar to the result obtained for the +DC component in non‐uniform electric field experiments.
First observation of a quadruple asteroid
Authors: Berdeu Anthony , Langlois Maud , Vachier Frédéric 
Published: January 2022

Context.Extreme adaptive optics systems, such as the Spectro-Polarimetric High-contrast Exoplanet REsearch facility (SPHERE), push forward the limits in high contrast and high resolution in direct imaging. The main objectives of these instruments are exoplanet detection and characterisation.Aims.We aim to increase the contrast limits to detect new satellites orbiting known asteroids. We use cutting-edge data reduction techniques and data processing algorithms that are essential to best analyse the raw data provided by the instruments and increase their performances. Doing so, the unequalled performances of SPHERE also make it a unique tool to resolve and study asteroids in the solar system, expanding the domain of its main science targets.Methods.We applied a newly developed data reduction pipeline for integral field spectrographs on archival SPHERE data of a resolved asteroid, (130) Elektra. It was coupled with a dedicated point spread function reconstruction algorithm to model the asteroid halo. Following the halo removal, the moon signal could be extracted more accurately. The moon positions were fitted at three epochs and were used to derive the orbital parameters via a genetic-based algorithm.Results.We announce the discovery of S/2014 (130) 2, a third moon orbiting (130) Elektra, making it the first quadruple asteroid ever found. It is identified in three different epochs, 9, 30, and 31 Dec. 2014, at a respective angular separation of 258 mas (333 km), 229 mas (327 km), and 319 mas (457 km). We estimate that this moon has a period of 0.679 ± 0.001 day and a semi-major axis of 344 ± 5 km, with an eccentricity of 0.33 ± 0.05 and an inclination of 38° ±19° compared to the primary rotation axis. With a relative magnitude to the primary of 10.5 ± 0.5, its size is estimated to be 1.6 ± 0.4 km.Conclusions.The orbital parameters of S/2014 (130) 2 are poorly constrained due to the unfavourable configurations of the available fragmentary data. Additional observations are needed to better estimate its orbit and to suggest a formation model. This new detection nonetheless shows that dedicated data reduction and processing algorithms modelling the physics of the instruments can push their contrast limits further.
Successful Secondary Endovascular Intervention in Pediatric Patients with Venous Thromboembolic Events
Authors: Glonnegger Hannah , Zieger Barbara , Grohmann Jochen , Freund Gabriele , Zeller Thomas , Uhl Markus , Stiller Brigitte 
Published: January 2022

Abstract
Background In the past, pediatric patients with venous thromboembolic events (VTE) were treated with low-molecular-weight heparin (LMWH) which was successful in around 70% of the cases. However, anticoagulation alone might not restore patency in all patients, and advanced therapeutic options to prevent postthrombotic syndrome are needed. During recent years, endovascular interventions have become a treatment option for pediatric patients with persistent thrombotic occlusion, not only in life- or limb-threatening VTE.
Methods We evaluated 12 consecutive patients (11–17 years) with newly diagnosed VTE being treated at our department during the last 4 years (2017–2020). In case follow-up examination showed persistent venoocclusion under anticoagulation, patients received secondary interventional therapy like recanalization, percutaneous transluminal angioplasty with or without catheter-directed thrombolysis, and stenting. Patients with no clinical signs of venoocclusion or regredient thrombosis in imaging examination received anticoagulation alone.
Results Six of 12 (50%) patients underwent catheter intervention. Median time from diagnosis to intervention was 4 months (0–12 months). Reintervention was necessary in one (8%) case and complete recanalization failed in one (8%) case. There were no major bleeding events or other major postinterventional complications, no acute or late local recurrence, and all patients reported clinical improvement after the procedure.
Conclusion If endovascular intervention is used in teenage patients with persistent symptomatic VTE, reduction of postthrombotic symptoms is possible, even if intervention is performed secondary to failure of anticoagulation. Multidisciplinary treatment decisions can be based on the clinical course and follow-up imaging.
Endoscopic Lumbar Discectomy Using Side-Viewing Conical Working Tube: An Institutional Experience
Authors: Kaif Mohammad , Yadav Kuldeep , Khan Khursheed Alam , Kumar Rakesh , Singh Deepak Kumar , Ahmad Faran 
Published: January 2022

Abstract
Objective The paradigm of surgical therapy for spinal disease especially for lumbar disc herniation (LDH) has gradually shifted from the traditional open surgeries to minimal invasive spinal surgeries. Endoscopic discectomy has been performed widely using various devices and techniques. In this study we present our experience of endoscopic discectomy using a unique device with separate side-viewing channel.
Methods Twenty-six patients with LDH treated between March 2015 and April 2018 using the unique conical working tube with separate side-viewing endoscopic channel have been retrospectively analyzed. Their preoperative and postoperative Oswestry Disability Index (ODI) and Macnab scores were used to evaluate the outcome with a mean follow-up of 37.04 months.
Results There were 18 males and 8 females with age ranging from 19 to 72 years (mean, 38.4 years). The follow-up ranged from 25 to 60 months with mean of 37.04 months. The mean preoperative ODI score was 72.4, which decreased to a mean of 7.6 and the outcome evaluated by Macnab criteria was 65.3% excellent, 19.2% good, 11.5% fair, and 3.8% poor. One patient underwent second surgery. None of the patients had to change their occupation postoperatively. Complications that occurred were dural tear in one patient and transient foot paresis in one, which improved spontaneously.
Conclusion Endoscopic discectomy using conical working tube is a safe and effective technique for lumbar disc prolapse. The long-term results are comparable to the conventional techniques.
History of Endoscopic Spine Surgery in India
Authors: Bajaj Jitin , Yadav Yad Ram 
Published: January 2022

AbstractEndoscopic spine surgeries provide distinct advantages and is therefore a viable alternative to open or microscopic techniques. Indian surgeons have shown their expertise from craniovertebral junction to lumbosacral spine with these techniques. Many novel approaches like endoscopic transcervical, partial corpectomy, and others have been designed, and many technological innovations for these surgeries have been made. With different training programs attracting both native and international surgeons, the future of endoscopic spine surgery is bright.
Hemorrhagic Brain Metastases: A Diagnostic Dilemma and Diagnosis of Exclusion
Authors: Meel Mukta , Bhaskar Mukesh Kumar , Jindal Arpita 
Published: January 2022

AbstractPleomorphic carcinoma is a malignant and aggressive primary lung carcinoma that occurs at a rate of approximately 0.3%. This rarely encountered tumor may present a diagnostic challenge to neuroradiologists and pathologists, as it has propensity to present as hemorrhagic brain metastasis with unknown primary and result in delay in diagnosis that could impact clinical outcome. Herein, we report a unique case in its presentation in a 56-year-old female, having symptomatic brain metastasis prior to the discovery of the pulmonary lesion.
Coiling Using Dual Microcatheter Technique—A Novel Approach
Authors: Kumar Arvind , Gandhi Swarup Sohan , Gandhi Ashok , Srivastav Trilochan , Purohit Devendra 
Published: January 2022

AbstractPosterior circulation aneurysms are difficult to treat, and if an incorporated artery is arising from the neck of aneurysm, management becomes much more challenging. Here, we are describing a novel technique used to treat a patient with a large, wide-necked left vertebral artery (VA)-posterior inferior cerebellar artery (PICA) junctional aneurysm. PICA seems to be arising from the aneurysm neck, but the aneurysm neck was not very clearly defined. So, we placed a second microcatheter into PICA, which not only allowed the coils to be placed in the aneurysm, without disrupting the flow through PICA but also helpful in assessing the aneurysmal occlusion. This technique allowed coils to be placed successfully without compromising flow through PICA.
Clinical Profile and Treatment Outcome of Spinal Epidural Arachnoid Cysts: A Systematic Review of Case Studies and Reports
Authors: Patil Aditya K. , Swain Srikant K. , Sharma Suresh , Arora Rajnish Kumar , Sharma Achal , Arora Poonam , Mittal Radhey S. 
Published: January 2022

Abstract
Background A spinal epidural arachnoid cyst (SEAC) is a rare clinical entity. We performed a systematic review of the literature to obtain information regarding demographics, clinical presentation, treatment modalities, and outcome of SEACs.
Methods A literature search was performed by using the databases PubMed/Medline, PubMed Central, Embase, Cochrane Library, Ovid MEDLINE, and Ovid Medline In-Process. A total of 170 articles were found on literature search. We found 575 cases of SEAC since 1904 for inclusion in the review including three cases which were operated by us. We studied the patient characteristics, clinical features, and management strategies, and evaluated their outcome.
Results The average age of presentation was 30 years with a male:female ratio of 1.03:1. They are commonly seen in the thoracic region (42.3%). The length of cyst was more than two vertebral levels in 85.81%. Mean symptom duration was 29 months, with most common presentation being that of compressive myelopathy. A good clinical outcome was present in symptomatic patients who had a shorter symptom duration and underwent complete surgical excision of the SEAC. Age, sex, length of lesion, and presence of dural defect did not have a bearing on the surgical outcome.
Conclusion For thoracic compressive myelopathy in a young patient, SEAC should be kept as a differential diagnosis. Surgical complete excision of the cyst with meticulous closure of the dural defect is the standard in management for a good clinical outcome.
Craniomegaly in Neonate and Infants Requiring Neurosurgical Intervention: An Experience at Tertiary Care Center
Authors: Mittra Sangh , Prajapati Hanuman Prasad , Kumar Raj 
Published: January 2022

Abstract
Background The identification of neurosurgical causes of craniomegaly and early institution of therapy requires for better clinical and functional outcomes.
Aims and Objectives The aim of this study was to evaluate the neurosurgical causes, managements, and outcomes of craniomegaly in neonate and infants.
Materials and Methods The cases with a history of head enlargement from neonatal period were included in this study. Their causes, managements, and outcomes were recorded retrospectively during the period of January 2010 to February 2013, in neurosurgery department at SGPGIMS Lucknow, and June 2018 to June 2020, at UPUMS, Saifai, Etawah, UP, India.
Results Out of 41 cases, there were 30 (73.14%) cases of hydrocephalus, 4 (9.76%) Dandy-Walker malformation, 2 (4.88%) subdural collection, 2 (4.88%) arachnoid cyst, 1 (2.44%) craniosynostosis, and 2 (4.88%) with tubercular meningitis. The age range of our cases was 18 to 178 days and the mean age was 102.54 ± 50.73. Preoperative head circumference range was 39 to 62 cm (mean: 55.27 ± 6.58cm). Majority of the cases (n = 32, 78.05%) were managed with ventriculoperitoneal shunt surgeries. Out of 41 cases, 33(80.49%) had improved outcomes, 7 (17.07%) stabilized, and mortality occurred in 1 (2.44%) case. Postoperatively, there was improvement in the head circumference (range: 39–60 cm and mean: 46.15 ± 5.83 cm) on 6 to 24 months (mean: 17.85 ± 5.18 months) of follow-up.
Conclusion Hydrocephalus was the commonest neurosurgical cause of head enlargement in neonate and infants. Shunt surgery was the most common form of management of these cases. Early detection, institution of therapy, and periodic follow-up program for diagnosing and treating complications were the key to successful outcomes in these patients.