bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recurrence of neovascular age-related macular degeneration after cessation of treat-and-extend regimen
Authors: Hirata Yuki , Oishi Akio , Maekawa Yuki , Tsuiki Eiko , Machida Akira , Kurihara Junko , Kitaoka Takashi 
Published: July 2022

Abstract
The appropriate timing of treatment cessation after treat and extend (TAE) regimen for age-related macular degeneration has not been established. This study aimed to investigate the incidence and risk factors of recurrence after cessation of the TAE regimen. We included patients who received and discontinued the TAE regimen, after extension of the treatment interval to > 12 weeks. The total number of injections, treatment period, last treatment interval, and visual acuity at treatment cessation were investigated as predictive factors. Forty-nine patients were included in the study. The estimated recurrence rates were 33% at one year and 48% at two years after treatment cessation, respectively. Higher chances of recurrence were associated with < 0.1 logarithm of the minimum angle of resolution (logMAR) at cessation (P < 0.002) and < 12 number of injections (P = 0.026). Meanwhile, five patients with visual acuity ≥ 1.0 logMAR at cessation did not show recurrence. Among the 25 recurrences, two lines of vision loss were noted in only two cases after resumed treatment. This study confirmed the importance of the number of injections in reducing recurrence and the association between visual acuity and recurrence. Recurrence is generally well-controlled with resumed treatment.
Evaluation of the relationship between hemoglobin A1C and the severity of carpal tunnel syndrome in diabetic patients
Authors: Hashemi Sayed Ebrahim , Sobhani Vahid , Asheghan Mahsa , Motaqi Monireh , Arabzadeh Ehsan , Boraghi Mahboobeh 
Published: July 2022

Abstract
Purpose
The aim of this study was to evaluation the relationship between hemoglobin A1C levels and the severity of carpal tunnel syndrome in diabetic patients.
Methods
In this cross-sectional study, 60 patients referred to endocrine and internal medicine clinics of Baqiyatallah Hospital who were diagnosed with diabetes and were receiving drug or insulin therapy were studied according to the Diagnostic criteria by the American Diabetes Association (ADA criteria). Patients' CTS severity was assessed according to HbA1C laboratory results and EMG / NCV test. Three questionnaires were completed for all participants: demographic information (height, weight, age, BMI, duration of diabetes, history of smoking and alcohol use, and history of other underlying diseases), Neuropathy disability score (NDS) (to assess nerve fiber damage, muscle strength, reflex loss, and loss of sensation), and Diabetic neuropathy symptoms (DNS) (for pain, numbness, tingling). The results were statistically analyzed.
Results
The study group consisted of 44 men and 16 women. 47 had type 2 diabetes and 13 had type 1 diabetes. The mean age of participants was 53.22 ± 7.28 years, the mean duration of diabetes was 11.2 ± 8.8 years, the mean duration of neuropathic symptoms was 2.9 ± 3.8 years, and the mean serum Hb A1C level of the subjects was 6.91 ± 1.38. The majority of subjects had mild CTS severity (30 out of 60). There was a statistically significant relationship between CTS severity and hemoglobin A1C levels in diabetic patients (P = 0.014).
Conclusion
The results of the study showed that the level of hemoglobin A1C has an effect on the severity of CTS. There was a statistically significant relationship between serum Hb A1C levels and CTS severity. The intensity of CTS increases with increasing hemoglobin A1C levels.
Molecular, clinicopathological characteristics and surgical results of resectable SMARCA4- deficent thoracic tumors
Authors: Luo Jizhuang , Ding Bowen , Campisi Alessio , Chen Tangbing , Teng Haohua , Ji Chunyu 
Published: July 2022

Abstract
PurposeSMARCA4 deficient thoracic tumors are rapid aggressive malignancies, often diagnosed at an advanced and inoperable stage. The value of pulmonary resection for resectable SMARCA4 deficient thoracic tumors are largely unknown. MethodsIn this study, we included 45 patients who received surgery for stage I-III SMARCA4 deficient tumors. We compared the molecular, clinicopathological characteristics and survival between SMARCA4-dNSCLC and SMARCA4-deficient undifferentiated tumor (SMARCA4-dUT) patients. ResultsThirty-four SMARCA4-dNSCLC and 11 SMARCA4-dUT patients were included in this study. Molecular profiles were available in 33 out of 45 patients. The most common mutated gene was TP53 (21, 64%), and followed by STK11(9, 27%), KRAS (5, 15%), FGFR1 (4, 12%) and ROS1 (4,12%). There were 3 patients harbored ALK mutation including 1 EML4-ALK rearrangement. There were 2 patients harbored EGFR rare site missense mutation. SMARCA4-dUT patients had significance worse PFS (HR=4.35 95%CI 1.77-10.71, p=0.001) and OS (HR=4.27, 95%CI 1.12-16.35, p=0.022) compared to SMARCA4-dNSCLC patients. SMARCA4-dUT histologic type, stage II/III, R1/2 resection and lymphovascular invasion were independent poor prognostic predictors for both PFS and OS. There were 8 patients received immunotherapy, the objective response rate was 50%. The SMARCA4-dNSCLC patient with ALK rearrangement was treated with crizotinib as second line therapy, and achieved stable disease for 9.7 months.ConclusionPatients with SMARCA4 deficient tumors have a high probability of early recurrence after surgery, except for stage I patients. Immunotherapy seems to be a valuable strategy to treat recurrence.
Improvement of bond behavior of fiber reinforced polymer bars using coarse sand and steel fibers: experimental study
Authors: Hassoon Alaa , Khalid Nibras , Shallal Mohanad 
Published: July 2022

Abstract
The use of fiber reinforced polymer composites as alternative to conventional steel reinforcement have been increased during last decades for their high tensile strength, light weight, and durability. This paper focused on the behavior of CFRP bars embedded in high strength concrete. Thirteen beam spacemen of 100×180×650 mm clear span were cast, each beam consist of two concrete blocks linked together. The parameters studied are: concrete strength, CFRP bar diameter, and two enhancement schemes; coarse sand and steel fibers applied directly to the surface area of the CFRP bars using epoxy adhesive. All beams are tested using four points loading system. The results indicates that the maximum bond stress and the strength ratio of CFRP bars enhanced using coarse sand particles or steel fibers increased by are 9.6 to 24.4% and 9.7 to 24.3% if compared with undeveloped bars due to the improved surface area which provides additional adhesive, friction resistance, and mechanical interlock. However, the bond index decreased up to 24.8%. Also, increasing of concrete compressive strength 55.38 MPa to 78.34 MPa increased the maximum bond stress and the strength ratio by 21.1 to 31.2% and 3.1 to 9% while the decreasing of the relative bond stress and bond index is 6 to 7% and 5.7 to 6.7%. Furthermore, using of CFRP bars of 10 mm diameter instead of 6 mm decreased the bond stress and the strength ratio by 18 to 27% and 42 to 44% due to the larger surface area and higher ultimate tensile strength.
Cytotoxicity and antitumor activity of organometallic copper (II) Nano particles in a chemically induced hepatocellular carcinoma rat model
Authors: El-Tabl Abdou S. , Wahed Moshira M. Abd-El , Abu-Setta Mohammed H.H. , El-Mahsarawy Al-Zahraa Ibrahim , Ashour Ahmed M. 
Published: July 2022

Abstract
This study determined the selective cytotoxicity and mutagenic potential of novel Schiff base complex nanoparticles of copper salt. Chemotherapeutic study of this complex nanoparticles both invitro and invivo in the treatment of hepatocellular carcinoma induced in rats had been done. The treatment efficacy of the studied complex nanoparticles was evaluated by measuring antioxidant activities against oxidative stress caused by diethyl nitrosamine in liver tissue. The measurements included reduced glutathione content, superoxide dismutase activity, total antioxidant capacity, as well as malondialdehyde level. Liver and kidney function tests were also determined, in addition to the evaluation of serum alpha-fetoprotein. Histopathological studies were also performed for liver, spleen, kidney, lung and testis tissues. Results showed that the metal complex nanoparticles had a high potency in the treatment of hepatocellular carcinoma which induced by diethyl nitrosamine in rats as it ameliorated from the investigated parameters toward normal control animals. These findings were well appreciated with histopathological studies of diethylnitrosamine group treated with the complex nanoparticles. It was found that the complex nanoparticles under study can interact with antioxidant to form cancer-specific proteasome inhibitors and apoptosis inducers in liver cancer cell.
Role of visual stimuli of a 3D sculpture tool in design cognition
Authors: Kim Jieun , Lee Jeehyun 
Published: July 2022

Abstract
This study focuses on the definitive role of visual stimuli of a 3D sculpture tool in cognitive design process. To analyze the role of visual stimuli by a 3D sculpture tool, this study performed a protocol analysis of analog design sketching and a 3D sculpture tool. A remarkable finding is that visual stimulation using the 3D sculpture tool expands transformative connections of thinking in design cognition. The transformative linkage influences on abstract ideas as extensible factors in the design process. Designers produce ideal sources, novel concepts, and develop initial obscure ideas through the process of transformative thinking. Extensible linkage was performed in the interaction of visual stimuli, imagination, and analogy. Transformative and extensible linkage for concept generation appeared after simulating design ideation sketching using the 3D sculpture tool, and strong visual metaphors were implemented through visual stimuli.
Circular RNA circRILPL1 promotes nasopharyngeal carcinoma malignant progression by activating the YAP/HIPPO signaling pathway
Authors: Wu Pan , Deng Xiangying , Wang Yian , Fan Chunmei , Hou Xiangchan , Mo Yongzhen , Wang Yuming , Li Zheng , Wang Fuyan , Guo Can , Zhou Ming , Liao Qianjin , Wang Hui , Zeng Zhaoyang , Jiang Weihong , Li Guiyuan , Xiang Bo , Xiong Wei 
Published: July 2022

Abstract
Background
Circular RNAs (circRNAs) play an important regulatory role in the pathogenesis and progression of nasopharyngeal carcinoma (NPC), but they have not been sufficiently studied.
Methods
The high expression of circRILPL1 in NPC tissues was confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH). The migration, invasion, proliferation, and mechanical properties of NPC cells were detected using wound healing, transwell, MTT, colony formation assays, and atomic force microscopy (AFM). Nude mice models including subcutaneous transplantation, tail vein-lung metastasis, and footpad-lymph node metastasis were established to determine the tumor-promoting effect of circRILPL1 in vivo. The proteomic analysis of circRILPL1 was performed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The interaction of circRILPL1 with ROCK1 and IPO7 was verified through RNA pull-down, RNA immunoprecipitation (RIP) and immunofluorescence-fluorescence in situ hybridization (IF-FISH). The expression of the HIPPO pathway-related proteins was examined using western blotting. The nucleocytoplasmic distribution of YAP was analyzed by immunofluorescence and cytosolic/nuclear fractionation. The transcriptional activity of YAP was detected by luciferase reporter assay. The effect of circRILPL1 on the enrichment of YAP on CAPN2 and PXN promoters was examined by chromatin immunoprecipitation.
Results
We revealed for the first time that circRILPL1 was up-regulated in NPC, weakened adhesion and decreased stiffness of NPC cells, and promoted NPC proliferation and metastasis in vitro and in vivo. Mechanistically, circRILPL1 inhibited the LATS1-YAP kinase cascade by binding to and activating ROCK1, resulting in decrease of YAP phosphorylation and degradation. Binding to and cooperating with transport receptor IPO7, circRILPL1 promoted the translocation of YAP from the cytoplasm to the nucleus, where YAP promoted the transcription of cytoskeleton remodeling genes CAPN2 and PXN. By which, circRILPL1 contributed to the pathogenesis of NPC.
Conclusions
Our results demonstrated that circRILPL1 promoted the proliferation and metastasis of NPC through activating the YAP/HIPPO signaling pathway by binding to both ROCK1 and IPO7. Highly expressed circRILPL1 in NPC may serve as an important biomarker for tumor diagnosis and also may be a potential therapeutic target.
Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species
Authors: Stenholm Åke , Hedeland Mikael , Pettersson Curt 
Published: July 2022

Abstract
In the search for methods to biodegrade recalcitrant compounds, the use of saprophytic fungi and white rot fungi, in particular belonging to the phylum Basidiomycota, has gained interest. This group of fungi possesses a battery of unspecific extracellular enzymes that can be utilized in the biodegradation of preferably phenolic compounds. In this work, it was investigated under which conditions the white rot fungus Trametes versicolor and the ericoid mycorrhizal fungus Rhizoscyphus ericae (belonging to the phylum Ascomycota) could be used to biodegrade the antibiotic aminoglycoside neomycin at co-metabolic conditions in which external nutrients were supplied. Furthermore, it was also investigated whether a biodegradation could be accomplished using neomycin as the sole nutrient. At selected conditions, the biodegradation of neomycin using Rhizoscyphus ericae was monitored by oxidation products of D-ribose which is a hydrolysis product of neomycin. The results show that both species can biodegrade neomycin 70% under co-metabolic conditions during an one-week time course and that Rhizoscyphus ericae is able to use neomycin as sole nutrient and to approximatively biodegrade it 60% under chosen non co-metabolic conditions. The results are of general interest in the search for fungal species that can biodegrade recalcitrant compounds without the need of external nutrients. The key future application area that will be investigated is purification of waste from recombinant protein production in which neomycin, nutrients and E. coli with neomycin resistance genes are present.
Premotor Function in Interpersonal Bimanual Coordination: Neural Responses to Varying Frequencies and Spatio-Temporal Relationships
Authors: Li Yanan , Niu Ruoyu , Liu Lei , Liu Ying 
Published: July 2022

Abstract
Background
Interpersonal movement coordination is an important aspect of daily life. Behavioral studies have found that rhythmic bimanual coordination of movement is mainly influenced by two factors, spatio-temporal relationship and frequency of movements. How these factors affect action coordination at the neural level needs further exploration.
Methods
Participants were asked to perform symmetric or alternating hand movements under conditions of different spatio-temporal relationships (symmetric, alternating) and frequencies. A multi-channel, continuous wave, functional near-infrared spectral (fNIRS) imaging instrument was used to monitor hemodynamic activity while 16 pairs of volunteers performed the task.
Results
Behaviorally, as indexed by phase locking value, movements were more stable in symmetric mode than in alternate mode. With increasing frequency, symmetric mode became more unstable; in contrast, alternating mode became more stable at higher frequencies, suggesting phase transition. Activation in brain regions of interest was much stronger in symmetric mode as compared with alternate mode. In alternate mode, but not symmetric mode, [HbO] varied with frequency.
Conclusion
Interpersonal bimanual coordination involves activity in premotor areas (premotor cortex, supplementary motor area, and frontal eye fields). More oxygen is consumed in these regions in alternating mode than in symmetric mode.
The relationship between Internet addiction and family environment among junior high school students in China
Authors: Jiang Zhongliang , Wang Zhiyi , Chen Jie , Tian Geng , Cheng Xiaojing , He Qiang , Zhao Miao , He Long , Liu Jintong 
Published: July 2022

Abstract
Objectives
Internet addiction is gaining attention because of the physical, psychological and social damage it can cause to individuals. There has been little systematic exploration of the relationship between Internet addiction and the influence of the family environment among junior high school students in China.
Methods
A whole-group sample of three county-level junior high schools in China was used to investigate the relationship between adolescents' Internet addictive behaviour and their family environment using the General Situation Questionnaire, Family Environment Scale-Chinese version, and the Internet Addiction Test (IAT).
Results
The prevalence of Internet addiction among junior high school students in China is 33.7%, among which 76.0% of junior high school students are mildly addicted to the Internet. The prevalence of Internet addiction among female students is 32.4%, and 35.0% among male students. the prevalence of Internet addiction among junior high school first year, junior high school second year and junior high school third year is 27.3%, 35.5% and 38%, respectively. The prevalence of Internet addiction among urban junior high school students is 27.5%, and the prevalence of Internet addiction among rural junior high school students is 34.5%. In terms of family environment, cohesion, expressiveness, independence, achievement, intellectual-cultural, active-recreational, moral-religious, organization, and control are negatively associated with Internet, family conflict is positively associated with Internet addiction (p < 0.01). The results of multiple stepwise regression analysis showed that seniority and prominent family conflict are risk factors for Internet addiction (P < 0.01), while female, good family expressiveness and organization, family intellectual-cultural, active-recreational and family control are prominent as protective factors for Internet addiction (P < 0.05).
Conclusion
Chinese junior high school students have a high prevalence of Internet addictive behaviours, with the highest proportion of mild Internet addictive behaviours. Males and senior students are more likely to develop Internet addictive behaviours. Family factors have a significant impact on the occurrence of Internet addiction, and interventions in the family environment of adolescents can effectively reduce the prevalence of Internet addictive behaviours.
Association of right precuneus compression with apathy in idiopathic normal pressure hydrocephalus: A case control study
Authors: Chadani Yoshihiro , Kashibayashi Tetsuo , Yamamoto Takahiro , Tsuda Atsushi , Fujito Ryoko , Akamatsu Masanori , Kamimura Naoto , Takahashi Ryuichi , Yamagami Takuji , Furuya Hirokazu , Ueba Tetsuya , Saito Motoaki , Inoue Keiji , Kazui Hiroaki 
Published: July 2022

Abstract
BackgroundIdiopathic normal pressure hydrocephalus (iNPH) is a slowly progressive syndrome caused by the excessive accumulation of cerebrospinal fluid and is typically characterized by the triad of cognitive, gait, and urinary disturbances and ventricular dilation. In addition to this triad of symptoms, apathy is frequently observed in iNPH and worsens the cognitive impairment and gait disturbance. Although apathy is an important symptom in iNPH, its neurological basis remains unknown.ObjectivesThis study aimed to analyze and elucidate the brain regions associated with apathy in iNPH using statistical imaging analysis and to investigate regional cerebral blood flow and regional gray matter volume, which indicates gray matter compression, in a whole-brain study.MethodsTwenty seven patients with iNPH were assigned to two groups based on their scores on the Neuropsychiatric Inventory items related with apathy; 18 patients were assigned to the group with apathy (iNPH + APA) and 9 to the group without apathy (iNPH - APA). The magnetic resonance images and cerebral blood flow single-photon emission computed tomography data of the two groups were compared using statistical parametric mapping 12. ResultsThe regional gray matter volume of the right precuneus was significantly larger in the iNPH + APA group than in the iNPH − APA group, but the and regional cerebral blood flow in any region of the brain was not significantly different between the two groups.ConclusionThese results suggested that compression of the precuneus might be involved in apathy in iNPH and the importance of brain compression in iNPH symptoms. In addition, this study also shows that VBM is useful for indicating the degree of brain compression.
Particle-tracking-based estimation of shear stress on cell aggregates in shaking vessel with different shaking methods/speeds
Authors: Horiguchi Ikki , Nagate Hotaka , Sakai Yasuyuki 
Published: July 2022

Abstract
Suspension cultures are widely used for cell expansion in regenerative medicine and production. Shear stress caused by the flow of medium in cultures causes cell death and delayed growth; thus, the shear stress on the cell surface must be minimized for successful suspension culture. We established a particle tracking system for estimating the shear stress on induced pluripotent stem cell aggregates during shaking culture and utilized it to optimize shaking conditions (orbital and reciprocating shaking). When the average accelerations of aggregates were calculated, high acceleration occurred periodically, and acceleration on the aggregates in orbital shaking was stable. Furthermore, the number of dead cells correlated with the time average of acceleration. In the case of growth, there was optimal acceleration due to various events, such as aggregate formation and cell death. These results indicated that image-based analyses of acceleration are helpful for estimating the shear stress on the aggregates in suspension culture to optimize agitation.
Field-based metabolomic reveals alteration of the volatiles organic compounds produced by Lavandula species in the context of yellow decline disease
Authors: Stierlin Émilie , Nicolè Florence , Costes Thomas , Mehl Florence , Fernandez Xavier , Michel Thomas 
Published: July 2022

Abstract
Introduction Fine lavender and lavandin are perfume and medicinal plants widely cultivated in Provence (south-east of France) for their essential oil and for the production of honey. The lavender monocultures are affected by a severe decline in France, due to the propagation of the yellow decline disease. This disease is caused by the Stolbur phytoplasma, a bacterium transmitted by a sap-sucking insect, Hyalesthes obsoletus. Objectives Main objective was to develop an untargeted metabolomic approach to highlight changes in the volatiles organic compounds produced by asymptomatic (“healthy”) and symptomatic (“infected”) plants. Methods Samples from fine lavender and lavandin aerial parts were collected in the field, then stored volatile compounds were extracted in the laboratory by ultrasound assisted extraction and analyzed by gas chromatography–mass spectrometry. Afterwards, multivariate statistical analysis has been performed using principal component analysis and partial least square regression as well as a multiblock statistical analysis by orthogonal partial least squares discriminant analysis to compare VOCs from fine lavender (variety 7713) and lavandin (variety abrial).Results Fifty stored VOCs, mainly oxygenated monoterpenes, from lavender and lavandin leaves and blooming head of the inflorescences were identified. Metabolism of several compounds were found to be altered by yellow decline such as β-farnesene overexpressed by symptomatic plants. Conclusion The developed metabolomic approach allowed the identification of specific volatile compounds produced by asymptomatic and symptomatic plants in a complex field environment.