bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular detection of Tritrichomonas foetus in bovine samples: a novel real-time polymerase chain reaction (PCR) assay targeting EF1-alpha-Tf1 and a comparative study of published PCR techniques
Authors: Polo Coral , García-Seco Teresa , Fernández Víctor , Hernández Marta , Briones Victor , Diez-Guerrier Alberto , Domínguez Lucas , Pérez-Sancho Marta 
Published: March 2022

Abstract
The parasite T. foetus causes trichomonosis in cattle but is generally asymptomatic in males. Thus, many bulls carrying the disease go unnoticed, making the detection of T. foetus in bulls an important aspect for its control. Due to drawbacks posed by its cultivation, PCR is a preferred option for diagnostic laboratories. Most published PCR protocols target the genomic region compring the 18S, 5.8S, and 28S rRNA genes and internal transcribed spacers 1 and 2 (rRNA-ITS region), homologous to that of other Tritrichomonas species. There is minimal information on alternative genetic targets and no comparative studies have been published. We compared a protocol based on the microsatellite TfRE (called H94) and five protocols based on the rRNA-ITS region (called M06, M15, G02, G05, and N02). We also designed and evaluated a novel PCR-based assay on the EF1-alpha-Tf1 gene (called V21). The analytical sensitivity and specificity assays for the PCR protocols were performed according to the World Organisation for Animal Health (OIE) directives and the comparative study was performed with a widely used PCR (M06) on clinical samples from 466 breeding bulls. V21 showed a high degree of agreement with our reference M06 (kappa = 0.967), as well as M15 (kappa = 0.958), G05 (kappa = 0.948), and H94 (kappa = 0.986). Protocols H94 and V21 appear to be good approaches for confirming clinical cases in preputial bull samples when genomic regions alternative to rRNA-ITS are required. By contrast, N02 gave false negatives and G02 false positives.
The association of unplanned pregnancy with perinatal depression: a longitudinal cohort study
Authors: Muskens Lotte , Boekhorst Myrthe G. B. M. , Kop Willem J. , van den Heuvel Marion I. , Pop Victor J. M. , Beerthuizen Annemerle 
Published: March 2022

Abstract
Perinatal depression is common, affecting approximately 7–13% of women. Studies have shown an association between unplanned pregnancy and perinatal depressive symptoms, but many used a cross-sectional design and limited postnatal follow-up. The current study investigated the association of unplanned pregnancy with perinatal depressive symptoms using a longitudinal cohort study that followed women from the first trimester until 12 months postpartum. Pregnant women (N = 1928) provided demographic and clinical data and information about pregnancy intention at the first trimester. Depressive symptoms were assessed during each trimester of pregnancy and five times postpartum using the Edinburgh Postnatal Depression Scale (EPDS) until 12 months postpartum. Mixed model analyses were used to investigate the association between an unplanned pregnancy and the level of depressive symptoms. Women with an unplanned pregnancy (N = 111, 5.8%) reported persistently higher levels of depressive symptoms during the entire perinatal period compared to women with a planned pregnancy, after adjustment for confounders (p < 0.001). However, the course of depressive symptom scores over time in women with an unplanned pregnancy was similar to that of women with a planned pregnancy. Lower age (p = 0.006), unemployment (p = 0.004), and history of depression (p < 0.001) were significantly associated with higher levels of perinatal depressive symptoms. An unplanned pregnancy may have a long-lasting negative impact on a woman’s perinatal mental health. Therefore, women with an unplanned pregnancy may benefit from systematic follow-up during the perinatal period with contingent mental health support.
Neuropathic pain after spinal intradural benign tumor surgery: an underestimated complication?
Authors: Butenschoen Vicki Marie , Nehiba Annika , Meyer Bernhard , Wostrack Maria 
Published: March 2022

Abstract
Neuropathic pain presents a burdening and impairing condition which may occasionally occur after spinal tumor surgery. While it has been described in peripheral nerve sheath tumors, data on other intradural tumor patients is sparse. We hereby present a large cohort population undergoing different intradural spinal tumor surgery with assessment of early postoperative and follow-up outcomes, focusing on the occurrence of neuropathic pain. We performed a retrospective monocentric study including all patients treated for intradural spinal tumors between 2009 and 2020. We extracted surgical aspects as well as pre- and postoperative clinical courses from the records. Statistical analysis of potential contributing prognostic factors was performed including matched pair analysis. In total, 360 patients were included for analysis. At a median follow-up of 2 years, 26/360 patients complained of a neuropathic pain syndrome (7.2%) requiring continuous medication. Of these patients only 50% complained preoperatively of pain. Tumor entity did not significantly influence the incidence of postoperative neuropathic pain (p = 0.91). Sacrifice of the tumor carrying nerve root and tumor recurrence also did not increase the risk for this condition. Persistent neuropathic pain requiring continuous treatment occurred in 7.2% of patients undergoing intradural spinal surgery in our cohort. This frequently underestimated postoperative adverse event represents a disabling condition leading to a substantial impairment in the quality of life among the affected patients.
Zur Rolle artenschutzrechtlicher Ausnahmen im Rahmen der raumordnerischen Konzentrationszonenplanung für die Windenergie: Ausnahmevoraussetzungen nach 45 Abs. 7 S. 2 BNatSchG und Regelhaftigkeit der Ausnahmeerteilung
Authors: Wagner Stephan 
Published: March 2022

ZusammenfassungAngesichts des ungebrochenen Ausbaubedarfs der Windenergie und zunehmend knapper werdender Standorte
rückt die artenschutzrechtliche Ausnahme nach 45 Abs. 7 BNatSchG mehr und mehr in den Fokus der Raumplanung.
In einem vorhergehenden Beitrag (NuR 2021, 803ff.) wurde die – mit Blick auf das Unionsrecht derzeit
nicht rechtssicher zu bejahende – Frage untersucht, ob für die Errichtung von Windkraftanlagen
ein Ausnahmegrund gemäß 45 Abs. 7 S. 1 BNatSchG gegeben ist. Im vorliegenden Beitrag werden
mit der Alternativlosigkeit und dem artenschutzrechtlichen Verschlechterungsverbot die weiteren Ausnahmevoraussetzungen
nach 45 Abs. 7 S. 2 BNatSchG sowie die für Planungspraxis und Projektträger nicht unwesentliche
Frage erörtert, ob und inwiefern die Ausnahmeerteilung durch die Naturschutzbehörden an eine gewisse
Regelhaftigkeit und damit Vorhersehbarkeit gebunden werden kann.
Closed-form perturbation theory in the restricted three-body problem without relegation
Authors: Cavallari Irene , Efthymiopoulos Christos 
Published: March 2022

AbstractWe propose a closed-form normalization method suitable for the study of the secular dynamics of small bodies in heliocentric orbits perturbed by the tidal potential of a planet with orbit external to the orbit of the small body. The method makes no use of relegation, thus circumventing all convergence issues related to that technique. The method is based on a convenient use of a book-keeping parameter keeping simultaneously track of all the small quantities in the problem. The book-keeping affects both the Lie series and the Poisson structure employed in successive perturbative steps. In particular, it affects the definition of the normal form remainder at every normalization step. We show the results obtained by assuming Jupiter as perturbing planet, and we discuss the validity and limits of the method.
Fire Severity Outcome Comparison of Apartment Buildings Constructed from Combustible and Non-Combustible Construction Materials
Authors: Zheng Alex , Garis Len , Pike Ian 
Published: March 2022

AbstractWood is commonly used in construction, but often perceived as being less safe than structures made from non-combustible materials. With the advancement of wood products and treatment, construction techniques, and protective systems, this may not be the case any longer. Using retrospective data from fire departments across Canada, this study aimed to determine whether the type of construction material (combustible or non-combustible) affected the fire severity outcome of a one to six storey apartment building fire, after accounting for protective systems (smoke alarms and sprinklers). The study found that, after adjusting for the presence of smoke alarms and sprinklers, structures constructed from non-combustible construction materials did not perform better in terms of injuries, requiring extinguishment by fire department, or the fire spreading beyond the room of origin. The presence of working smoke alarms and sprinklers played a central role in reducing the severity outcome of a fire. Smoke alarms and sprinklers both reduced the odds of extinguishment by the fire department and the fire spreading beyond the room of origin. Sprinklers also reduced the injury rate. Overall, this study highlighted the importance of protective systems in reducing fire severity outcomes.
Long-lasting effects of experimental flow intermittency on alpine stream macroinvertebrates (Val Roseg, Switzerland)
Authors: Drost Annemieke M. , Siebers Andre R. , Paillex Amael , Misteli Benjamin , Peeters Edwin T. H. M. , Robinson Christopher T. 
Published: March 2022

AbstractChanging weather patterns and receding glaciers are predicted to increase flow intermittency in alpine streams. If aquatic macroinvertebrate communities largely comprise taxa adapted to perennial flows, an increase in flow intermittency substantially reduces biodiversity and affects functional processes. We conducted a before-after-control-impact field experiment to examine how macroinvertebrate communities in an alpine headwater stream responded to and recovered from a repeated experimental increase in flow intermittency. Flow in one channel was manipulated to simulate increased summer intermittency (June–September) over two consecutive years, whilst an adjacent channel served as a control. We monitored the density of benthic macroinvertebrates, periphyton and organic matter at approximately monthly intervals over three years during the snow-free period. Before manipulation, both channels had similar ecological properties. The flow manipulation reduced the overall macroinvertebrate density, and especially the proportional rheophile density, across both years. Recovery of the macroinvertebrate community following experimental flow intermittency took more than a year, and longer than our study period. This could be due to long aquatic life stages, dispersal limitation and biotic interactions. We conclude that climate-induced changes in alpine stream flow regimes can lead to a fundamental shift in macroinvertebrate assemblages through local extinctions, mostly of rheophilic species.
Stable isotope analysis reveals trophic segregation between the invasive zebra mussel Dreissena polymorpha and the native duck mussel Anodonta anatina in Lake Trasimeno (Italy)
Authors: Ludovisi Alessandro , Goretti Enzo , Pallottini Matteo , Lucentini Livia , Pizzirani Claudia , Vizzini Salvatrice , Mancinelli Giorgio 
Published: March 2022

AbstractNon-indigenous freshwater bivalves negatively affect invaded ecosystems through different mechanisms, including inter-specific competition for trophic resources. Here, we investigated in Lake Trasimeno (Central Italy) the diet of the invasive Dreissena polymorpha and the native Anodonta anatina. δ15N and δ13C stable isotopes were measured in winter and summer in bivalves, phytoplankton, and sedimentary organic matter (SOM); the relative dietary contributions of the two resources were determined using Bayesian mixing models. To elucidate the different carbon and nitrogen pools characterizing the study site, isotopic analyses were extended to zooplankton and to representatives of the benthic flora and macroinvertebrate fauna. Independently from the season, the two bivalves showed a limited trophic overlap, as mixing models indicated for D. polymorpha a diet based primarily on phytoplankton, while A. anatina relied mainly on SOM. Dietary differences were less marked in summer, when comparable isotopic values characterized phytoplankton and SOM. In winter, conversely, the trophic differentiation between the two species was more evident, and corresponded with a significant enrichment in SOM δ13C values, likely due to a substantial contribution of carbon deriving from decaying macrophytes. Whether differences in ecological and behavioral traits alone can explain the observed trophic segregation between the two species, or if they have actively shifted their diet to reduce competition for food is discussed. We conclude emphasizing the need of an advanced resolution of the influence of non-indigenous species on the flux of energy and matter in invaded lentic systems, including Lake Trasimeno.
Adolescent Mental Health Resilience and Combinations of Caregiver Monitoring and Warmth: A Person-centred Perspective
Authors: Theron Linda , Rothmann Sebastiaan , Makhnach Alexander , Ungar Michael 
Published: March 2022

AbstractCaregiver monitoring and warmth have protective mental health effects for adolescents, including vulnerable adolescents. However, combinations of the aforesaid parenting behaviours and their relationship with adolescent mental health are underexplored, especially among younger and older South African (SA) adolescents challenged by structural disadvantage. Hence, the purpose of this study was to investigate unique profiles of caregiver monitoring and warmth and their associations with depression and conduct problems as reported by younger and older adolescents from disadvantaged SA communities. Latent profile and linear regression analyses were used to examine cross-sectional survey data generated by 891 adolescents from two disadvantaged SA communities (62.2% aged 13–17 [average age: 16.13]; 37.5% aged 18–24 [average age: 20.62]). Two profiles emerged. The first, i.e. substantial caregiver warmth and some monitoring, was associated with younger and older adolescent reports of statistically significantly fewer symptoms of depression and conduct problems. The second, i.e. caregiver monitoring without much warmth, was associated with significantly more symptoms of depression or conduct problems among younger and older adolescents. Traditional gender effects (i.e. higher depression symptoms among girls; higher conduct problem symptoms among boys) were amplified when caregiver monitoring was combined with low warmth. In short, protecting the mental health of younger and older adolescents from disadvantaged communities requires higher levels of caregiver warmth combined with moderate levels of caregiver supervision. Because stressors associated with disadvantaged communities jeopardise warm parenting, supporting caregiver resilience to those stressors is integral to supporting adolescent mental health.
Optimal WACC in tariff regulation under uncertainty
Authors: Romeijnders Ward , Mulder Machiel 
Published: March 2022

AbstractIn the regulation of network tariffs, the compensation for the opportunity costs of capital through the Weighted Average Costs of Capital (WACC) plays a crucial role. Determining the appropriate level for the WACC is, though, problematic because of the uncertainty about the future conditions in capital markets. When the WACC is set above the future opportunity costs of capital, consumers will pay too much, while when the WACC is below that level, network operators may be unable to finance investments affecting quality of network services. In this paper, we explicitly take this uncertainty into account when we determine the optimal WACC for the tariff regulation of an electricity network. By trading off consumer surplus and expected disruption costs in the electricity grid, we conclude that from a social-welfare perspective in most cases the optimal WACC in tariff regulation is above the historical mean costs of capital. Only in case of high uncertainty about the true costs of capital while network operators are able to quickly increase investment levels, the optimal WACC is below the historical mean because then it is less likely that the WACC is constantly insufficient to cover actual costs of capital. However, when network operators cannot quickly increase investment levels the optimal WACC is always above the historical mean cost of capital.
Exploring the Origin of Stealth Coronal Mass Ejections with Magnetofrictional Simulations
Authors: Bhowmik P. , Yeates A. R. , Rice O. E. K. 
Published: March 2022

AbstractCoronal mass ejections (CMEs) – among the most energetic events originating from the Sun – can cause significant and sudden disruption to the magnetic and particulate environment of the heliosphere. Thus, in the current era of space-based technologies, early warning that a CME has left the Sun is crucial. Some CMEs exhibit signatures at the solar surface and in the lower corona as the eruption occurs, thus enabling their prediction before arriving at near-Earth satellites. However, a significant fraction of CMEs exhibit no such detectable signatures and are known as “stealth CMEs”. Theoretical and observational studies aiming to understand the physical mechanism behind stealth CMEs have identified coronal streamers as potential sources. In this paper, we show that such streamer-blowout eruptions – which do not involve the lift-off of a low-coronal magnetic flux rope – are naturally produced even in the quasi-static magnetofrictional model for the coronal magnetic field. Firstly, we show that magnetofriction can reproduce in this way a particular stealth CME event observed during 1 – 2 June 2008. Secondly, we show that the magnetofrictional model predicts the occurrence of repeated eruptions without clear low-coronal signatures from such arcades, provided that the high, overlying magnetic field lines are sufficiently sheared by differential rotation. A two-dimensional parameter study shows that such eruptions are robust under variation of the parameters, and that the eruption frequency is primarily determined by the footpoint shearing. This suggests that magnetofrictional models could, in principle, provide early indication – even pre-onset – of stealth eruptions, whether or not they originate from the eruption of a low-coronal flux rope.
A Study Examining the Usefulness of a New Measure of Research Engagement
Authors: Bowen Deborah J. , Ackermann Nicole , Thompson Vetta Saunders , Nederveld Andrea , Goodman Melody 
Published: March 2022

Abstract
Introduction
Engagement of relevant stakeholders’ ideas, opinions, and concerns is critical to the success of modern research projects. We have developed a tool to measure stakeholder engagement, called the Research Engagement Survey Tool (REST). The purpose of this paper is to present the implementation and uptake of the stakeholder engagement measure REST among research teams, including the assessment of barriers and facilitating factors for use of the new research engagement measure in practice.

Methods
In this implementation study, project team members participated in baseline and follow-up web-based surveys. Web-based interviews were conducted with a subset of project teams that implemented the REST. On the baseline survey, project teams were asked to provide details about up to three ongoing or recently completed projects, were asked if they agreed with compensation for REST completion, and were asked if they would like to send the survey to stakeholders or would prefer our project team to email their project stakeholders. Follow-up surveys contained questions on reactions to implementing REST and results of REST.

Results
Project team members/researchers who completed the baseline survey (n=86) were mostly female (79%) and Non-Hispanic/Latino(a) White (76%). Those who implemented REST were also mostly female (86%) and Non-Hispanic/Latino(a) White (71%), with an average of 11 years in academic research. About 98% of all participants completing the baseline survey had the capacity to survey partners, while 100% of all teams who implemented REST did. A small portion of respondents indicated the time commitment of REST would be a barrier (29% of baseline survey respondents, 10% of those who implemented REST) and indicated workload would be a barrier (31% of baseline survey respondents, 14% of those who implemented REST).

Discussion
The data presented here indicate that REST implementation is feasible in a volunteer group of ongoing research projects.
An End-User’s Personal Perspective on the Need of Consumer Involvement in Research
Authors: Ferry S. M. , Henzel M. Kristi , Bogie Kath M. 
Published: March 2022

AbstractThere is growing understanding that the consumer’s voice in research needs to be stronger. Translational research studies need consumer inclusion in order to be effectively implemented. This narrative article provides the perspective of a Veteran with spinal cord injury (SCI) who is an active member of several study teams and serves as a Consumer Advocate, providing the voice of the person with SCI. Factors that drive people to develop new research ideas are considered. Consumer involvement offers helpful insight into project outcomes that are valuable to the end-user. It is also recognized that data can be interpreted in several different ways depending on the observer. Including the consumer in a research project enables another interpretation, creating a more complete evaluation. Participating in health research is becoming a new standard for persons with many different illnesses and diseases. Greater things are accomplished by physicians, healthcare scientists, engineers, and healthcare consumers interacting together to increase both the quality of research projects and the quality of life for everyone involved, especially the person with the disorder. There will be more acceptance of ideas or projects when consumers are involved from the early steps and learn how the process works from beginning to end.