bluuebunny/crossref_metadata_2025_split huggingface dataset:
Species identification by MALDI-TOF MS and gap PCR–RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis
Authors: Rosa Nives Maria , Penati Martina , Fusar-Poli Sara , Addis Maria Filippa , Tola Sebastiana 
Published: October 2022

AbstractStaphylococci and streptococci are common causes of intramammary infection in small ruminants, and reliable species identification is crucial for understanding epidemiology and impact on animal health and welfare. We applied MALDI-TOF MS and gap PCR–RFLP to 204 non-aureus staphylococci (NAS) and mammaliicocci (NASM) and to 57 streptococci isolated from the milk of sheep and goats with mastitis. The top identified NAS was Staphylococcus epidermidis (28.9%) followed by Staph. chromogenes (27.9%), haemolyticus (15.7%), caprae, and simulans (6.4% each), according to both methods (agreement rate, AR, 100%). By MALDI-TOF MS, 13.2% were Staph. microti (2.9%), xylosus (2.0%), equorum, petrasii and warneri (1.5% each), Staph. sciuri (now Mammaliicoccus sciuri, 1.0%), arlettae, capitis, cohnii, lentus (now M. lentus), pseudintermedius, succinus (0.5% each), and 3 isolates (1.5%) were not identified. PCR–RFLP showed 100% AR for Staph. equorum, warneri, arlettae, capitis, and pseudintermedius, 50% for Staph. xylosus, and 0% for the remaining NASM. The top identified streptococcus was Streptococcus uberis (89.5%), followed by Strep. dysgalactiae and parauberis (3.5% each) and by Strep. gallolyticus (1.8%) according to both methods (AR 100%). Only one isolate was identified as a different species by MALDI-TOF MS and PCR–RFLP. In conclusion, MALDI-TOF MS and PCR–RFLP showed a high level of agreement in the identification of the most prevalent NAS and streptococci causing small ruminant mastitis. Therefore, gap PCR–RFLP can represent a good identification alternative when MALDI-TOF MS is not available. Nevertheless, some issues remain for Staph. haemolyticus, minor NAS species including Staph. microti, and species of the novel genus Mammaliicoccus.
Generation of mitochondria-rich kidney organoids from expandable intermediate mesoderm progenitors reprogrammed from human urine cells under defined medium
Authors: Yuan Yapei , Chen Huan , Ou Sihua , Cai Baomei , Zhang Ruifang , Qin Yue , Pan Mengjie , Cao Shangtao , Pei Duanqing , Hou Fan Fan 
Published: October 2022

AbstractBackgroundThe kidneys require vast amounts of mitochondria to provide ample energy to reabsorb nutrients and regulate electrolyte, fluid, and blood pressure homeostasis. The lack of the human model hinders the investigation of mitochondria homeostasis related to kidney physiology and disease.ResultsHere, we report the generation of mitochondria-rich kidney organoids via partial reprogramming of human urine cells (hUCs) under the defined medium. First, we reprogrammed mitochondria-rich hUCs into expandable intermediate mesoderm progenitor like cells (U-iIMPLCs), which in turn generated nephron progenitors and formed kidney organoids in both 2D and 3D cultures. Cell fate transitions were confirmed at each stage by marker expressions at the RNA and protein levels, along with chromatin accessibility dynamics. Single cell RNA-seq revealed hUCs-induced kidney organoids (U-iKOs) consist of podocytes, tubules, and mesenchyme cells with 2D dominated with mesenchyme and 3D with tubule and enriched specific mitochondria function associated genes. Specific cell types, such as podocytes and proximal tubules, loop of Henle, and distal tubules, were readily identified. Consistent with these cell types, 3D organoids exhibited the functional and structural features of the kidney, as indicated by dextran uptake and transmission electron microscopy. These organoids can be further matured in the chick chorioallantoic membrane. Finally, cisplatin, gentamicin, and forskolin treatment led to anatomical abnormalities typical of kidney injury and altered mitochondria homeostasis respectively.ConclusionsOur study demonstrates that U-iKOs recapitulate the structural and functional characteristics of the kidneys, providing a promising model to study mitochondria-related kidney physiology and disease in a personalized manner.
Accuracy of clinicians’ ability to predict the need for renal replacement therapy: a prospective multicenter study
Authors: Sitbon Alexandre , Darmon Michael , Geri Guillaume , Jaubert Paul , Lamouche-Wilquin Pauline , Monet Clément , Le Fèvre Lucie , Baron Marie , Harlay Marie-Line , Bureau Côme , Joannes-Boyau Olivier , Dupuis Claire , Contou Damien , Lemiale Virginie , Simon Marie , Vinsonneau Christophe , Blayau Clarisse , Jacobs Frederic , Zafrani Lara 
Published: October 2022

Abstract
Purpose
Identifying patients who will receive renal replacement therapy (RRT) during intensive care unit (ICU) stay is a major challenge for intensivists. The objective of this study was to evaluate the performance of physicians in predicting the need for RRT at ICU admission and at acute kidney injury (AKI) diagnosis.

Methods
Prospective, multicenter study including all adult patients hospitalized in 16 ICUs in October 2020. Physician prediction was estimated at ICU admission and at AKI diagnosis, according to a visual Likert scale. Discrimination, risk stratification and benefit of physician estimation were assessed. Mixed logistic regression models of variables associated with risk of receiving RRT, with and without physician estimation, were compared.

Results
Six hundred and forty-nine patients were included, 270 (41.6%) developed AKI and 77 (11.8%) received RRT. At ICU admission and at AKI diagnosis, a model including physician prediction, the experience of the physician, SOFA score, serum creatinine and diuresis to determine need for RRT performed better than a model without physician estimation with an area under the ROC curve of 0.90 [95% CI 0.86–0.94, p < 0.008 (at ICU admission)] and 0.89 [95% CI 0.83–0.93, p = 0.0014 (at AKI diagnosis)]. In multivariate analysis, physician prediction was strongly associated with the need for RRT, independently of creatinine levels, diuresis, SOFA score and the experience of the doctor who made the prediction.

Conclusion
As physicians are able to stratify patients at high risk of RRT, physician judgement should be taken into account when designing new randomized studies focusing on RRT initiation during AKI.
Identifying existing approaches used to evaluate the sustainability of evidence-based interventions in healthcare: an integrative review
Authors: Flynn Rachel , Stevens Bonnie , Bains Arjun , Kennedy Megan , Scott Shannon D. 
Published: October 2022

Abstract
Background
There is limited evidence to evaluate the sustainability of evidence-based interventions (EBIs) for healthcare improvement. Through an integrative review, we aimed to identify approaches to evaluate the sustainability of evidence-based interventions (EBIs) and sustainability outcomes.

Methods
Following Whittemore and Knafl’s methodological process: (1) problem identification; (2) literature search; (3) data evaluation; (4) data analysis; and (5) presentation, a comprehensive search strategy was applied across five databases. Included studies were not restricted by research design; and had to evaluate the sustainability of an EBI in a healthcare context. We assessed the methodological quality of studies using the Mixed Methods Appraisal Tool.

Results
Of 18,783 articles retrieved, 64 fit the inclusion criteria. Qualitative designs were most commonly used for evaluation (48%), with individual interviews as the predominant data collection method. Timing of data collection varied widely with post-intervention data collection most frequent (89%). Of the 64 studies, 44% used a framework, 26% used a model, 11% used a tool, 5% used an instrument, and 14% used theory as their primary approach to evaluate sustainability. Most studies (77%) did not measure sustainability outcomes, rather these studies focused on sustainability determinants.

Discussion
It is unclear which approach/approaches are most effective for evaluating sustainability and what measures and outcomes are most commonly used. There is a disconnect between evaluating the factors that may shape sustainability and the outcomes approaches employed to measure sustainability. Our review offers methodological recommendations for sustainability evaluation research and highlights the importance in understanding mechanisms of sustainability to advance the field.
A service composition method using improved hybrid teaching learning optimization algorithm in cloud manufacturing
Authors: Zeng Jun , Yao Juan , Gao Min , Wen Junhao 
Published: October 2022

AbstractIn the cloud manufacturing process, service composition can combine a single service into a complex service to meet the task requirements. An efficient service composition strategy is crucial, as it affects the efficiency of resource and capacity sharing in the cloud manufacturing system. However, in the face of a large-scale environment, the existing methods have the problems of slow convergence and instability. To solve the problems above, we propose an improved optimization method, named improved-TC. Specifically, we are inspired by the horizontal crossover of CSO in the hybrid-TC teaching phase, the Hybrid-TC is proposed in our previous work, which is a hybrid of the teaching-learning-based optimization algorithm (TLBO) and the crisscross optimization algorithm (CSO). Improved-TC is an improvement on the learning phase of hybrid-TC algorithm, we change the search method of hybrid-TC in the learning phase to a one-dimensional search, thereby some dimensions in the population that are trapped in the local optimum have the chance to jump out of the iteration. Experiments show that our proposed method has a faster convergence speed and more stability in the face of service composition in large-scale environments.
Assessment of prescribing errors reported by community pharmacy professionals
Authors: Simegn Wudneh , Weldegerima Berhanemeskel , Seid Mohammed , Zewdie Ayal , Wondimsigegn Dawit , Abyu Chilot , Kasahun Asmamaw Emagn , Seid Abdulwase Mohammed , Sisay Gashaw , Yeshaw Yigizie 
Published: October 2022

Abstract
Background
Medication errors have serious consequences for patients’ morbidity and mortality. The involvement of pharmacy professionals in the prescribing and dispensing procedure allowed the detection of a range of drug-related problems in addition to identification by prescribers. They are often the first point of contact in the healthcare system in identifying prescribing errors and intervening in these errors by dealing with the prescribers and the patients.

Objectives
This study aimed to assess prescribing errors reported by community pharmacy professionals in Gondar Town, North West Ethiopia.

Methods
A self-administered cross-sectional survey was employed from February 29 to June 23, 2020, to collect data on prescribing errors reported by community pharmacy professionals. All community pharmacy professionals found in Gondar town were included. Community pharmacy professionals who were ill at the time of study and who had less than 6 months of work experience were excluded.

Results
Seventy-four pharmacy professionals participated in the study with a response rate of 93.6%. The overall prevalence of prescribing errors was 75.1% (95% CI 71.08–78.70). Of these errors, drug selection was the most common (82.4%), followed by errors of commission (79.7%) and errors of omission (78.4%). Antibiotics (63.5%) were commonly involved in prescribing errors, followed by analgesics (44.5%) and antipsychotics (39.5%).

Conclusion
The findings of this study revealed a high prevalence of prescribing errors in Gondar, Ethiopia. Drug selection was the most prescribed error, followed by errors of commission. Stakeholders should design interventions such as training, integrating prescribers with clinical pharmacists and supervising interns by seniors. Large-scale studies that include potential factors of prescribing problems are recommended for future researchers.
Potential of UK and US newspapers for shaping patients' knowledge and perceptions about antidiabetic medicines: a content analysis
Authors: Syafhan Nadia Farhanah , Chen Gaoyun , Parsons Carole , McElnay James C. 
Published: October 2022

Abstract
Background
Information about how newspapers portray antidiabetic medicines to readers is lacking. This study investigated the reporting on antidiabetic medicines in the most widely circulated newspapers published in the United Kingdom (UK) and the United States (US) over a 10-year period.

Methods
The Nexis UK database was used to identify and select relevant articles. Systematic content analysis of the articles which met the inclusion criteria (articles of any format that contained reference to antidiabetic medicines) within the highest circulated newspapers in the UK and US between 2009 and 2018 was conducted. Inter-rater reliability of coding was established using a 10% sample of the identified articles.

Results
A total of 560 (369 UK and 191 US) relevant newspaper articles were retrieved. In the UK, the number of relevant articles showed a slightly increasing trend over the study period, while in the US, article numbers declined over the study period. Safety/risk of antidiabetic medicines was the most frequent theme covered by the articles (34.6%). Over one-third of the newspaper articles were written from a clinical perspective (37.7%). Insulin was the most commonly discussed class of antidiabetic medicine (23.1%). Control of blood sugar levels (53.1%) and side effects/toxicity (92.7%) were the most frequently reported benefit and risk of antidiabetic medicines, respectively. The most frequently reported organ systems harmed by antidiabetic medicines were the cardiovascular, endocrine and gastrointestinal systems. The UK newspapers were more likely to report the benefits of antidiabetic medicines (p = 0.005), while the US articles were more likely to report on harms/risks (p = 0.001). The majority of relevant articles (91.8%) were judged as having a balanced judgement, while 8.2% of the articles were rated as exaggerated.

Conclusions
This study has revealed that antidiabetic medicines are indeed reported on by UK and US newspapers. As media portrayal has the potential to negatively or positively influence patients’ views of their medication for diabetes, healthcare professionals should check on patients’ beliefs and knowledge about their medication and proactively provide objective and balanced information (including promotion of medication adherence).
Bispectral index: the current tool for monitoring unintended awareness and depth of anesthesia
Authors: Chhanwal Heena , Kheskani Divya , Gandhi Parita , Shah Meet Bhadresh , Makwana Devendra , Rathod Vasu 
Published: October 2022

Abstract
Background
Awareness under general anesthesia is an unpleasant phenomenon that usually goes unnoticed and neglected. Numerous incidences of intraoperative awareness are not reported. Reasons for awareness might be the inadequate depth of anesthesia, less effective drugs, lack of proper anesthesia monitoring equipment, and untrained medical staff.
The purpose of this study is to evaluate intraoperative awareness during general anesthesia and titrate the amount of anesthetic agents according to BIS values among patients and monitor hemodynamic parameters throughout the surgery.

Results
The intraoperative awareness reported was 2% in the BIS group and 8% in the non-BIS group. The total propofol consumption in the BIS group was significantly less as compared to the non-BIS group (P value<0.0001).

Conclusions
The incidence of definite awareness with postoperative recall and propofol consumption was reduced in the BIS group as compared to the non-BIS group.
Design and implementation of a simple and inexpensive respiratory synchronization control platform
Authors: Doyle John 
Published: October 2022

Abstract
Background
In a number of clinical and research settings, it is desirable to have an individual breathe in a particular fixed pattern (respiratory synchronized breathing). The purpose of this brief technical report is to show how a control system for this purpose can be easily and inexpensively developed using an Arduino UNO microcontroller platform.

Results
We programmed an Arduino UNO microcontroller to develop a respiratory timing system with selectable respiratory rate and inspiratory to expiratory ratio. Test subjects are instructed to breathe in when the light-emitting diode (LED) is illuminated and breathe out when the LED is dark. Both the duration of inspiration and that of expiration can be easily adjusted by the user to meet various requirements. The system was tested and found to function satisfactorily.

Conclusions
An Arduino UNO microcontroller was used to develop a respiratory timing system. This platform is likely to be of value to clinicians and investigators looking for a simple and inexpensive system for respiratory synchronized breathing.
Impact of older age at Fontan completion on mid-term survival
Authors: Aurora Ruth Grace , Prakoso Radityo , Fakhri Dicky , Sakidjan Indriwanto , Siagian Sisca Natalia , Almazini Prima , Lilyasari Oktavia 
Published: October 2022

Abstract
Background
The optimum age of Fontan completion remains unknown. Currently, the majority of centers worldwide are performing Fontan completion at 2–4 years of age. In Indonesia, lack of awareness and limited resources probably explain why patients seek treatment at advanced stage. This study aimed to evaluate the impact of older age at Fontan completion on mid-term survival.

Results
A single-center retrospective cohort study was performed on 261 patients who underwent Fontan completion between 2008 and 2019 and survived to discharge. The patients were followed up until April 2020, with a median follow-up period of 3 years (range 0–12 years). The median age was 5 years (range 2–24 years). The survival rates of patients with the age at operation ≤ 6 years and > 6 years were 92.1% and 82.8%, respectively. A subgroup analysis showed that the survival rates for age < 4 years, 4–6 years (reference age), 6–8 years, 8–10 years, 10–18 years, and > 18 years were 85.7%, 94.8%, 85.4%, 78.8%, 85.7%, and 66.7%, respectively. Age at Fontan completion of > 6 years (HR 3.84; p = 0.020) was associated with a lower 12-year survival rate. The age at operation of 8–10 years (HR 6.79; p = 0.022) and > 18 years (HR 15.30; p = 0.006) had the worst survival rates.

Conclusions
An older age at Fontan completion (> 6 years) significantly reduced mid-term survival rate. The age at Fontan of 8–10 years and > 18 years had higher risk of mid-term death than age of 4–6 years.
Diet-induced insulin resistance altered cardiac GLUT4 and FATP/CD36 expression in rats
Authors: Afolabi Oladele Ayobami , Alabi Babatunde Adebola , Oluranti Olufemi 
Published: October 2022

Abstract
Background
Altered substrate transport protein expression is central to the effect of insulin resistance on cardiac metabolism. The present study was thus designed to investigate the comparative effects of high fat, high sucrose and salt-induced IR on cardiac expression of fatty acid transporter (FATP) and glucose transporter (GLUT4) in rats.

Results
Rats fed with high fat, high sucrose and salt diets developed impaired glucose tolerance (p > 0.05) and hyperinsulinemia (p < 0.05) compared with control group. Myocardial glucose transporter expression was significantly increased (p < 0.001 for salt-induced IR; p < 0.01 for sucrose-induced IR; p < 0.01 for fat-induced IR) across all IR groups compared with control. Fatty acid transporter expression was also increased (p < 0.001) in high salt diet-induced IR rats, and high fat diet-induced IR rats (p < 0.05).

Conclusions
Our results demonstrate that salt and not caloric excess has a potential role in IR alteration of myocardial substrate transport protein expression in the rat.
Comparison of serum IGF1, IGF2 and IGFBP1-6 concentration in the children with different stages of autism spectrum disorders
Authors: Mashayekhi Farhad , Shabani Somayeh , Salehi Zivar 
Published: October 2022

Aim of the studyAutism spectrum disorder (ASD) is a neurodevelopmental condition considered by early-onset difficulties in social communication. Insulin-like growth factors (IGFs) play crucial roles in synapse formation. Most of circulating IGFs are bound to IGF-binding proteins (IGFBPs) that modify IGF action. IGFBPs prolong the plasma half-life of IGFs. In this project we studied the association of IGF1/2 and IGFBP1-6 serum concentration with the severity of ASD (Levels 1-3; Mild, Moderate and severe, respectively).Subject or material and methodsA hundred and eighty patients with ASD (Mild; n=69, Moderate; n=58 and Severe; n=53) and 118 controls age matched were used in this project and IGF1/2 and IGFBP1-6 serum concentration were measured by ELISA.ResultsThe results demonstrated that IGF1, IGF2 and IGFBP1-6 were present in all serum samples. The results showed that the concentration of IGF1 and IGF2 was significantly higher in ASD patients when compared to controls, starting from stages I to III ASD, a significant increase of IGF1 and IGF2 serum concentration was observed. Results obtained also showed that IGFBP1-6 concentration in the ASD group were lower when compared to controls and low serum IGFBP1-6 concentration is associated with advanced stages of ASD.DiscussionIGFs plays important role in synapse formation and changes in IGFs expression may be important in the pathogenesis of ASD.ConclusionsIt is suggested that IGFs and IGFBPs may be involved in the pathogenesis of ASD. Therefore, the detection of serum soluble IGFs and IGFBPs may be useful in classifying ASD.
Temperament and character as a predictor of response to selective serotonin reuptake inhibitors in patients with Major depressive disorder
Authors: Nadi Sakhvidi Mohammad , Namdari Mahshid , Shayegh Samira , Hosseini Fatemeh , Kholaseh zadeh Golrasteh , Bozorg Bonnie , Salimi Zanireh , Amini Fariba , Bidaki Reza 
Published: October 2022

Aim of the studyPersonality is one of the most important factors affecting the treatment course of patients with psychiatric disorders. The present study aimed to find the possible relationship between personality factors and response to selective serotonin reuptake inhibitors treatment for major depression.Subject or material and methodsIn the present prospective study, one hundred and seven patients with mild or moderate depression treated with citalopram, sertraline or fluoxetine for two months. Every patients were evaluated by Hamilton depression test (as pre-test and post-test) and Temperament and Character Inventory (as pre-test) and their response to treatment evaluated base on their Hamilton depression test. The collected data were analyzed using ANOVA, chi- square, Pearson's correlation coefficients tests and logistic regression.ResultsThe mean age of the patients was 39.7 years and most of the population were female (71.9%). The results showed that decrease in RD (OR=0.85, P=0.05) and age (OR=0.93, P=0.003), and increase in CO score (OR=1.32, P<0.001) had a significant effect on the likelihood of being responsive to the treatment.DiscussionThe present study demonstrated that the cooperativeness could be considered as an appropriate predictor of treatment response among responders and non-responders. The investigation of four groups revealed that those with complete response had higher cooperativeness and lower novelty seeking while the non-responsive group had higher Persistence and self-transcendence.ConclusionsTemperament and character or at least some of their traits may predispose response to depression treatment.