bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interleukin-18 and High-Mobility-Group-Protein B1 are Early and Sensitive Indicators for Cell Damage During Normothermic Machine Perfusion after Prolonged Cold Ischemic Storage of Porcine Liver Grafts
Authors: Beetz Oliver , Cammann Sebastian , Weigle Clara A. , Sieg Lion , Eismann Hendrik , Johanning Kai , Falk Christine S. , Krech Till , Oldhafer Felix , Vondran Florian W. R. 
Published: October 2022

In the era of organ machine perfusion, experimental models to optimize reconditioning of (marginal) liver grafts are needed. Although the relevance of cytokine signatures in liver transplantation has been analyzed previously, the significance of molecular monitoring during normothermic machine perfusion (NMP) remains elusive. Therefore, we developed a porcine model of cold ischemic liver graft injury after prolonged static cold storage (SCS) and subsequent NMP: Livers obtained from ten minipigs underwent NMP for 6 h directly after procurement (control group) or after 20 h of SCS. Grafts after prolonged SCS showed significantly elevated AST, ALT, GLDH and GGT perfusate concentrations, and reduced lactate clearance. Bile analyses revealed reduced bile production, reduced bicarbonate and elevated glucose concentrations after prolonged SCS. Cytokine analyses of graft perfusate simultaneously demonstrated an increase of pro-inflammatory cytokines such as Interleukin-1α, Interleukin-2, and particularly Interleukin-18. The latter was the only significantly elevated cytokine compared to controls, peaking as early as 2 h after reperfusion (11,012 ng/ml vs. 1,493 ng/ml; p = 0.029). Also, concentrations of High-Mobility-Group-Protein B1 were significantly elevated after 2 h of reperfusion (706.00 ng/ml vs. 148.20 ng/ml; p &lt; 0.001) and showed positive correlations with AST (r2 = 0.846) and GLDH (r2 = 0.918) levels. Molecular analyses during reconditioning of liver grafts provide insights into the degree of inflammation and cell damage and could thereby facilitate future interventions during NMP reducing acute and chronic graft injury.
High-Throughput Transcriptomic Profiling Reveals the Inhibitory Effect of Hydroquinine on Virulence Factors in Pseudomonas aeruginosa
Authors: Rattanachak Nontaporn , Weawsiangsang Sattaporn , Daowtak Krai , Thongsri Yordhathai , Ross Sukunya , Ross Gareth , Nilsri Nungruthai , Baldock Robert A. , Pongcharoen Sutatip , Jongjitvimol Touchkanin , Jongjitwimol Jirapas 
Published: October 2022

Hydroquinine is an organic alkaloid compound that exhibits antimicrobial activity against several bacterial strains including strains of both drug-sensitive and multidrug-resistant P. aeruginosa. Despite this, the effects of hydroquinine on virulence factors in P. aeruginosa have not yet been characterized. We therefore aimed to uncover the mechanism of P. aeruginosa hydroquinine-sensitivity using high-throughput transcriptomic analysis. We further confirmed whether hydroquinine inhibits specific virulence factors using RT-qPCR and phenotypic analysis. At half the minimum inhibitory concentration (MIC) of hydroquinine (1.250 mg/mL), 254 genes were differentially expressed (97 downregulated and 157 upregulated). We found that flagellar-related genes were downregulated by between −2.93 and −2.18 Log2-fold change. These genes were consistent with the analysis of gene ontology and KEGG pathway. Further validation by RT-qPCR showed that hydroquinine significantly suppressed expression of the flagellar-related genes. By analyzing cellular phenotypes, P. aeruginosa treated with ½MIC of hydroquinine exhibited inhibition of motility (30–54% reduction) and pyocyanin production (~25–27% reduction) and impaired biofilm formation (~57–87% reduction). These findings suggest that hydroquinine possesses anti-virulence factors, through diminishing flagellar, pyocyanin and biofilm formation.
The Ritualization of Classic Confucian Spirit of Jing (Reverence and Respect): Evidence from Traditional Chinese Capping Ritual
Authors: Wu Yue , Liang Hui’e , Shen Yijun , Jiang Qianling 
Published: October 2022

In ancient China, all moral concepts are based on Li 禮 (ritual). Jing 敬 (reverence and respect) is one of the core categories of Confucian ritual spirituality and has rich ideological connotations. This study discusses how Confucianism realizes the ritualization of jing and constructs its symbolic system in the capping ritual to strengthen adult consciousness and social responsibility. First, based on relevant classic texts, we clarify the internal relationship between traditional ritual spirituality and jing. Then, we present an overview of the coming-of-age ceremony and discuss how religious beliefs and rituals incorporate Confucian ethical values and aesthetics. Finally, from the ritual uses of time, space, and behavior, we examine the meaning of jing in the specific practice of the traditional Chinese capping ritual and how it is conveyed to participants and observers through ritual implements and behaviors. The results show the capping ritual as an important life etiquette, and Confucianism injects the spirit of jing into every phase to cultivate an emotional response that will instantiate a moral ideal applicable to individuals and the state. In complex, modern societies, it is important to condense the Confucian spiritual connotation of jing and integrate it into modern coming-of-age rites.
Redesigning New Policy Options for Thalassemia Prevention in Sri Lanka
Authors: Amarasinghe Nadeeja , Amarasena Amila , Thabrew Anoj , Werawatte Prabhath , Premawardhena Anuja , Malik Farnaz , Abusayeed Mohamed , Wickramasinghe Champika 
Published: October 2022

Sri Lanka, a country with 22 million people, has nearly 2000 thalassemia patients with severe thalassemia, two-thirds of whom have beta thalassemia major (TM). The current prevention program based on promoting “safe marriages”, which has been in existence for over 15 years, has failed to reduce thalassemia major births. We set about to examine the cost-effectiveness of novel policy options for thalassemia prevention in Sri Lanka. Methods: The current cost for treatment of a thalassemia major patient (USD 2602/yr) was compared against the cost per reduction of single birth with three novel strategies, namely intensifying the screening in the current five districts combined with an education program (policy option 1), a nationwide screening program (policy option 2), and antenatal screening combined with the termination of pregnancy (policy option 3). The incremental cost-effectiveness ratio (ICER) of the different strategies was calculated. Results: The status quo was considered to reduce one TM birth whilst the new policy options were able to reduce births by 14, 35, and 48, respectively. The costs incurred for the program for a year for status quo and the three novel programs were USD 104,788, 173,884, 781,372, and 904,186 respectively. Cost per prevention of a thalassemia major birth was USD 87,324, 12,420, 22,324, and 20,084, respectively. The lifetime cost per treatment of a thalassemia major patient was USD 34,653. Conclusions: Given the current legal restriction on termination of pregnancy for fetal indications, policy option 2, an island-wide screening with mass education, is the most cost-effective and will be expected to deliver a substantial reduction in new births.
Metode Material Requirement Planning (MRP) untuk Perencanaan Kebutuhan Bahan Baku pada Produksi Songkok Nasional di UD. Iqbal VIP
Authors: Rufaidah Anik , Lestari Kurnia Cahya , Wahyudin Muhammad Arif 
Published: October 2022

The right production design at a company will get effectiveness and more production efficiency, including the plan for material requirements or raw materials used by the company. In essence, planning to meet the needs of the material in a company is expected to produce an appropriate system to be applied, therefore making the production process smooth. Where this research aims to compile Material Requirement Planning (MRP) with lot-sizing technique, then compare the results to obtain the optimal combination of raw material procurement. The use of time series method on-demand uses past data, namely the last two years and the forecasting results use the additive decomposition method because this method gets a minimum error value. By using POM, the demand prediction for one year is 6951 scores. Meanwhile, the MPS results on the raw material for one year of songkok required 488 pcs of velvet cloth, 1391 kg of pelisir and 6951 scores of bos-bosan. From the MRP results, the PPB technique is the most optimal for planning the raw material needs of velvet, lining and boredom by ordering the purchase of natural velvet fabric as much as six times, pelisir 1 time and bos-bosan as 6 times.
Impact of undoped channel thickness and carbon concentration on AlN/GaN-on-SiC HEMT performances
Authors: Harrouche Kathia , Venkatachalam Srisaran , Grandpierron François , Okada Etienne , Medjdoub Farid 
Published: October 2022

Abstract
We report on a vertically scaled AlN/GaN high electron mobility transistor technology design optimization for millimeter-wave applications. The undoped GaN channel thickness and carbon concentration into the buffer are extensively varied and systematically characterized. It is found that a thin GaN channel, typically below 150 nm improves the electron confinement, but increases the trapping effects, especially when using shorter gate lengths. Moreover, high carbon concentration into the buffer enables not only high electron confinement but also low leakage current under a high electric field at the expense of trapping effects. As a result, the optimum epi-design enabled state-of-the-art RF performances at 40 GHz.
EFECTO DEL MALESTAR EMOCIONAL SOBRE EL EMPODERAMIENTO DE LA ENFERMEDAD EN MUJERES QUE VIVEN CON DIABETES
Authors: Medina-Hernández Josué , Ruíz-Lara Anahí , Rascón-Arriaga Fernanda Guadalupe , Méndez-Domínguez María Isabel , Canté-Hernández Debora , Torres-Soto Nissa Yaing 
Published: October 2022

El objetivo del estudio es explicar el efecto del malestar sobre el empoderamiento en mujeres que viven con diabetes. Diseño correlacionalexplicativo, con un muestreo a conveniencia a 387 mujeres. Se aplicó el instrumento Cuestionario de área de problemas en diabetes (α=0.90) y la Escala de empoderamiento en diabetes (α=0.89). Se realizó estadística descriptiva e inferencial como correlación de Spearman y modelo de ecuaciones estructurales. Se halló relación entre el malestar emocional y la glucosa capilar (p<0.05), y relación de la edad (r=p<0.05) y malestar emocional (p<0.05) con el empoderamiento en diabetes. En el modelo estructural el malestar emocional afecta negativamente el empoderamiento de la diabetes. Se concluye que el malestar emocional produce un efecto negativo en el empoderamiento de la diabetes, por lo que este grupo vulnerable requiere un abordaje especifico que mejore sus procesos emocionales, permitiendo un mejoramiento del control glucémico.
PENGARUH PENERAPAN MODEL PEMBELAJARAN KOOPERATIF TIPE MAKE A MATCH UNTUK MENINGKATKAN HASIL BELAJAR BIOLOGI SISWA DI SMAN 4 BUKITTINGGI
Authors: Juwantika Iga Claudia , Hendri Wince , Deswati Lisa 
Published: October 2022

Penelitian ini bertujuan untuk mengetahui pengaruh penerapan model pembelajaran kooperatif tipe Make A Match terhadap hasil belajar biologi siswa yang dilaksanakan pada siswa kelas X MIPA SMAN 4 Bukittinggi Tahun Ajaran 2016/2017. Metode penelitian ini adalah eksperimen. Subyek penelitian adalah siswa kelas X MIPA SMAN 4 Bukittinggi dengan sampel penelitian berjumlah 60 orang yang diambil menggunakan teknik purposive sampling danmenghasilkan dua kelas sampel yang terdiri atas kelas eksperimen dan kelas kontrol. Teknik pengumpulan data adalah soal tes akhir untuk hasil belajar ranah kognitif. Analisis data yang digunakan adalah uji normalitas, homogenitas dan hipotesis. Hasil penelitian menunjukkan penerapan model pembelajaran kooperatif tipe Make A Match mempengaruhi hasil belajar biologi siswa dengan rata-rata hasil belajar kelas eksperimen 74.67 lebih baik daripada rata-rata hasil belajar kelas kontrol 68.67. Berdasarkan uji hipotesis yang telah dilakukan dengan ?=0.05 diperoleh thitung(2.34) lebih besar dari ttabel(1.68). Saran dari penelitian ini adalah diharapkan dalam pelaksanaan model pembelajaran kooperatif tipe Make A Match diberikan intruksi model ini lebih diperjelas dengan rinci supaya siswa tidak bingung dalam melakukan pembelajaran dengan model pembelajaran ini.
MENINGKATKAN MINAT BELAJAR SISWA MIN 2 SAMBAS MENGGUNAKAN PEMBELAJARAN KOOPERATIF TIPE STAD
Authors: Soramiranda Nizar 
Published: October 2022

Siswa yang berusia 7 sampai 11 tahun, berada pada tahap operasional konkret. Siswa belum mampu melakukan penalaran sesuatu yang sifatnya abstrak. Teman sebaya memiliki peranan yang sangat penting dalam perkembangan sosial siswa, karena melalui teman sebaya, siswa bisa belajar dan mendapat informasi baru. Siswa merasa sulit dalam pelajaran matematika yang bersifat abstrak. Akibatnya minat belajar siswa pada pelajaran matematika rendah.
Rumusan masalahnya adalah upaya meningkatkan minat belajar siswa menggunakan STAD. Tujuannya untuk mengetahui penerapan STAD dalam meningkatkan minat belajar siswa. Indikator keberhasilan adalah persentase minat belajar siswa mencapai 75% (baik).
Hasil penelitian perhatian siswa 68,7% (cukup) menjadi 81,2% (baik). Ketertarikan siswa 65% (cukup) menjadi 85% (baik). Kemauan siswa 68,7% (cukup) menjadi 81,2% (baik). Rasa senang 66,7% (cukup) tetap 66,7% (cukup). Rata-rata hasil penelitian dari 67,27% (cukup) menjadi 78,52% (baik).
FEATURES OF CAD/CAE SYSTEMS APPLICATION IN THE CONTEXT OF EXTENDING RESOURCE OF THE FLAME TUBE OF THE MAIN COMBUSTION CHAMBER OF THE TURBOJET ENGINE OF TACTICAL AVIATION AIRCRAFT
Authors: Shulhin A. , Karasov O. , Chemerys Y. , Bashynskyi K. , Kholodnyi R. 
Published: October 2022

The article conducted a modeling number of the working process in the flame tube of the main chamber of combustion of the turbojet engine of tactical aviation aircraft. The geometric model of the flame tube has been created and adapted for carrying out the interconnecting calculation of heat and stress-strain stations of the walls of the flame tube under the influence of operating factors at the numerical modeling of the working process. Quantitative-qualitative analysis of modeling results was carried out and the connection between peculiarities of working process flow and characteristic damage of the flame tubes discovered in operation was established. The possibility of using modern CAD/CAE systems to solve scientific tasks to ensure maximum use of resource opportunities of the main and especially responsible parts of turbojet engune on the basis of the assessment of their damage.
The obtained results of work process numerical calculations, thermal state and VAT of the heat pipes walls of the main combustion chamber refuse:

identify critical areas and perform optimal construction;
the decision to establish or extend the heat pipes resource is substantiated based on the assessment of the degree of depletion of harmfulness in conditions where the developer and manufacturer do not fulfill their obligations in terms of author support;
to provide recommendations to aircraft repair companies regarding the finalization of aircraft structures during the implementation of the overhaul, avoiding long-term experiments to determine their ultimate state.
Characterization of Errors in Retinopathy of Prematurity Diagnosis by Ophthalmologists-in-Training in Middle-Income Countries
Authors: Patel Samir N. , Al-Khaled Tala , Kang Kai B. , Jonas Karyn E. , Ostmo Susan , Ventura Camila V. , Martinez-Castellanos Maria A. , Anzures Rachelle Go Ang Sam , Campbell J. Peter , Chiang Michael F. , Chan R.V. Paul 
Published: October 2022

Purpose:
To characterize common errors in the diagnosis of retinopathy of prematurity (ROP) among ophthalmologistsin-training in middle-income countries.

Methods:
In this prospective cohort study, 200 ophthalmologists-in-training from programs in Brazil, Mexico, and the Philippines participated. A secure web-based educational system was developed using a repository of more than 2,500 unique image sets of ROP, and a reference standard diagnosis was established by combining the clinical diagnosis and the image-based diagnosis by multiple experts. Twenty web-based cases of wide-field retinal images were presented, and ophthalmologists-in-training were asked to diagnose plus disease, zone, stage, and category for each eye. Trainees' responses were compared to the consensus reference standard diagnosis. Main outcome measures were frequency and types of diagnostic errors were analyzed.

Results:

The error rate in the diagnosis of any category of ROP was between 48% and 59% for all countries. The error rate in identifying type 2 or pre-plus disease was 77%, with a tendency for overdiagnosis (27% underdiagnosis vs 50% overdiagnosis; mean difference: 23.4; 95% CI: 12.1 to 34.7;
P
= .005). Misdiagnosis of treatment-requiring ROP as type 2 ROP was most commonly associated with incorrectly identifying plus disease (plus disease error rate = 18% with correct category diagnosis vs 69% when misdiagnosed; mean difference: 51.0; 95% CI: 49.3 to 52.7;
P
= .003).

Conclusions:
Ophthalmologists-in-training from middle-income countries misdiagnosed ROP more than half of the time. Identification of plus disease was the salient factor leading to incorrect diagnosis. These findings emphasize the need for improved access to ROP education to improve competency in diagnosis among ophthalmologists-in-training in middle-income countries.

[
J Pediatr Ophthalmol Strabismus
. 2023;60(5):344–352.]
Multifunctional NK Cell–Engaging Antibodies Targeting EGFR and NKp30 Elicit Efficient Tumor Cell Killing and Proinflammatory Cytokine Release
Authors: Klausz Katja , Pekar Lukas , Boje Ammelie Svea , Gehlert Carina Lynn , Krohn Steffen , Gupta Tushar , Xiao Yanping , Krah Simon , Zaynagetdinov Rinat , Lipinski Britta , Toleikis Lars , Poetzsch Sven , Rabinovich Brian , Peipp Matthias , Zielonka Stefan 
Published: October 2022

Abstract
In this work, we have generated novel Fc-comprising NK cell engagers (NKCEs) that bridge human NKp30 on NK cells to human epidermal growth factor receptor (EGFR) on tumor cells. Camelid-derived VHH single-domain Abs specific for human NKp30 and a humanized Fab derived from the EGFR-specific therapeutic Ab cetuximab were used as binding arms. By combining camelid immunization with yeast surface display, we were able to isolate a diverse panel of NKp30-specific VHHs against different epitopes on NKp30. Intriguingly, NKCEs built with VHHs that compete for binding to NKp30 with B7-H6, the natural ligand of NKp30, were significantly more potent in eliciting tumor cell lysis of EGFR-positive tumor cells than NKCEs harboring VHHs that target different epitopes on NKp30 from B7-H6. We demonstrate that the NKCEs can be further improved with respect to killing capabilities by concomitant engagement of FcγRIIIa and that soluble B7-H6 does not impede cytolytic capacities of all scrutinized NKCEs at significantly higher B7-H6 concentrations than observed in cancer patients. Moreover, we show that physiological processes requiring interactions between membrane-bound B7-H6 and NKp30 on NK cells are unaffected by noncompeting NKCEs still eliciting tumor cell killing at low picomolar concentrations. Ultimately, the NKCEs generated in this study were significantly more potent in eliciting NK cell–mediated tumor cell lysis than cetuximab and elicited a robust release of proinflammatory cytokines, both features which might be beneficial for antitumor therapy.
Akt-2 Is a Potential Therapeutic Target for Disseminated Candidiasis
Authors: Huang Ling , Ma Yilei , Guo Hui , Tang Na , Ouyang Song , Nuro-Gyina Patrick , Tao Lijian , Liu Yusen , O’Brien Matthew C , Langdon Wallace Y , Zhang Jian 
Published: October 2022

Abstract
Akt-1 and Akt-2 are the major isoforms of the serine/threonine Akt family that play a key role in controlling immune responses. However, the involvement of Akt-1 and Akt-2 isoforms in antifungal innate immunity is completely unknown. In this study, we show that Akt2−/−, but not Akt1−/−, mice are protected from lethal Candida albicans infection. Loss of Akt-2 facilitates the recruitment of neutrophils and macrophages to the spleen and increases reactive oxygen species expression in these cells. Treating C57BL/6 mice with a specific inhibitor for Akt-2, but not Akt-1, provides protection from lethal C. albicans infection. Our data demonstrate that Akt-2 inhibits antifungal innate immunity by hampering neutrophil and macrophage recruitment to spleens and suppressing oxidative burst, myeloperoxidase activity, and NETosis. We thus describe a novel role for Akt-2 in the regulation of antifungal innate immunity and unveil Akt-2 as a potential target for the treatment of fungal sepsis.
STAT3 Inhibits Autocrine IFN Signaling in Type I Conventional Dendritic Cells
Authors: Chrisikos Taylor T , Zhou Yifan , Kahn Laura M , Patel Bhakti , Denne Nina L , Brooks Athena , Shen Li , Wang Jing , Watowich Stephanie S 
Published: October 2022

Abstract
Type I conventional dendritic cells (cDC1s) are an essential Ag-presenting population required for generating adaptive immunity against intracellular pathogens and tumors. While the transcriptional control of cDC1 development is well understood, the mechanisms by which extracellular stimuli regulate cDC1 function remain unclear. We previously demonstrated that the cytokine-responsive transcriptional regulator STAT3 inhibits polyinosinic:polycytidylic acid [poly(I:C)]-induced cDC1 maturation and cDC1-mediated antitumor immunity in murine breast cancer, indicating an intrinsic, suppressive role for STAT3 in cDC1s. To probe transcriptional mechanisms regulating cDC1 function, we generated novel RNA sequencing datasets representing poly(I:C)-, IL-10–, and STAT3-mediated gene expression responses in murine cDC1s. Bioinformatics analyses indicated that poly(I:C) stimulates multiple inflammatory pathways independent of STAT3, while IL-10–activated STAT3 uniquely inhibits the poly(I:C)-induced type I IFN (IFN-I) transcriptional response. We validated this mechanism using purified cDC1s deficient for STAT3 or IFN signaling. Our data reveal IL-10–activated STAT3 suppresses production of IFN-β and IFN-γ, accrual of tyrosine phosphorylated STAT1, and IFN-stimulated gene expression in cDC1s after poly(I:C) exposure. Moreover, we found that maturation of cDC1s in response to poly(I:C) is dependent on the IFN-I receptor, but not the type II IFN receptor, or IFN-λ. Taken together, we elucidate an essential role for STAT3 in restraining autocrine IFN-I signaling in cDC1s elicited by poly(I:C) stimulation, and we provide novel RNA sequencing datasets that will aid in further delineating inflammatory and anti-inflammatory mechanisms in cDC1s.