bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between the reflection magnitude and blood pressure in a multiethnic cohort: the Healthy Life in an Urban Setting study
Authors: Bouwmeester Thomas A. , van de Velde Lennart , Galenkamp Henrike , Postema Pieter G. , Westerhof Berend E. , van den Born Bert-Jan H. , Collard Didier 
Published: August 2022

Aims:
Reflection magnitude (RM), the ratio of the amplitudes of the backward and forward central arterial pressure waves, has been shown to predict cardiovascular events. However, the association with blood pressure (BP) and hypertension is unclear.

Methods:
We assessed RM in 10 195 individuals of Dutch, South-Asian Surinamese, African Surinamese, Ghanaian, Turkish and Moroccan origin aged between 18 and 70 years (54.2% female) participating in the Healthy Life in an Urban Setting study. To determine RM, central arterial pressure and flow were reconstructed from finger BP. Hypertension was defined based on office-BP and medication. Associations with BP, hypertension, and hypertensive organ damage were assessed using linear regression models with correction for relevant covariates.

Results:
Mean RM was 62.5% (standard deviation [SD] 8.0) in men and 63.8% (SD 8.1) in women. RM was lowest in Dutch and highest in South-Asian and African participants. RM increased linearly with 1.35 (95% confidence interval [CI] 1.23–1.46) for every 10 mmHg increase in systolic BP from 120 mmHg onwards, while the relation with diastolic BP was nonlinear. RM was 2.40 (95% CI 2.04–2.76) higher in hypertensive men and 3.82 (95% CI 3.46–4.19) higher in hypertensive women compared to normotensive men and women. In hypertensive men and women with ECG-based left ventricular hypertrophy or albuminuria RM was 1.64 (95% CI 1.09–2.20) and 0.94 (95% CI 0.37–1.52) higher compared to hypertensive participants without hypertensive organ damage.

Conclusion:
RM is associated with BP, hypertension and hypertensive organ damage, and may in part explain disparities in hypertension associated cardiovascular risk.
Exploring Meaning of Life in Women With Breast Cancer in Taiwan: A Phenomenological Study
Authors: SUN Fan-Ko , LONG Ann , TSENG Yun Shan , YAO YuChun 
Published: August 2022

ABSTRACT

Background
Globally, breast cancer is the most common cancer type in terms of incidence for women. Women with breast cancer endure higher levels of psychological distress than other types of cancer because many lose their identity as a woman, which is an additional characteristic of their psychological distress. Research using phenomenology to explore “the meaning of life” is rare among women with breast cancer.

Purpose
The purpose of this study was to explore the perspectives of women with breast cancer on “the meaning of life.”

Methods
A phenomenological approach was used. Twenty-six women living with breast cancer drawn from a cancer treatment hospital in Taiwan participated in this study. A semistructured interview was utilized to collect the data, and Colaizzi's seven steps were used to analyze the data.

Results
Four themes emerged: (a) value of overcoming suffering, (b) value of reciprocal love, (c) value of self-transcendence, and (d) value of spiritual comfort. This means that the participants defined “the meaning of life” through the lens of suffering from cancer, reciprocal love from their families and friends, uncovering and discovering creative pathways that transformed their pain while searching for the value of their existence, and seeking spiritual guidance from religion.

Conclusions
The participants identified the most pivotal aspect of healing as transforming their pain and accepting value for their suffering. They acknowledged they could not change the fact that they had cancer, but they could learn to accept it as part of their lived experience. Healthcare professionals may use these four themes at a clinically appropriate time on women's journeys toward healing to inspire women with breast cancer to process their own unique “meaning of life.”
Role of dynamic and parametric whole-body FDG PET/CT imaging in molecular characterization of primary breast cancer: a single institution experience
Authors: Sundaraiya Sumati , T Raja , Nangia Sapna , Sirohi Bhawna , Patil Sushama 
Published: August 2022

Aim
The aim of this pilot study was to assess the role of dynamic whole-body PET and parametric imaging in the biological characterization of primary breast cancer.

Materials and method
In total 24 histologically proven primary breast cancer lesions in 21 consecutive patients were retrospectively analyzed. Each patient underwent 18F-fluoro-deoxyglucose whole-body dynamic PET-CT before any treatment. Dynamic PET images were acquired in the list mode for a total duration of 70 min. The reconstructed parametric imaging generated Patlak plot-based ‘Slope’ and ‘Intercept’ images, from which parametric indices ki and DV were obtained. The standard uptake value (SUV) metric was also obtained by summing the last few frames of the dynamic study. ki, distribution volume (DV) and SUV were correlated with the histological tumor grade, biomarkers [hormone receptors and human epidermal growth factor receptor 2 (HER-2) neu expression] and molecular subtypes (A, B and C) as well as with tumor size, regional nodal metastases and distant metastases.

Results
The mean ki was found to be significantly higher in grade III than II lesions (P = 0.005), HER-2 neu positive status (P = 0.04) and molecular subtype B (P = 0.04) as well as in greater than T1 lesions(P = 0.0003 and P = 0.04, respectively) and node-positive lesions (P = 0.009). Though mean ki was not found to be significant for the hormone receptors status (P = 0.08), it showed the best correlation compared to the other parameters (P = 0.8 for DV and P = 0.1 for SUV). Spearman’s correlation test, area under the curve (AUC) and mismatch percentage also revealed ki to predict tumor grade (AUC, 0.95; r = 0.7; P = 0.0001), HER-2 neu status and molecular subtypes (AUC, 0.81; r = 0.49 and P = 0.01) along with the hormone receptors status (AUC, 0.83; r = 0.32; P = 0.1). The mean DV failed to show any association with any of the biological or anatomical staging parameters. Though ki was found to be comparable to that of SUV in almost all the assessed parameters, it appeared to be better for predicting hormone receptors status even though both parameters were not statistically significant.

Conclusion
Our initial observation in a small cohort of breast cancer patients suggests that ki is promising in stratifying primary breast cancer lesions according to the tumor grade and biological characteristics.
Effect of vitamin E and supragingival scaling on salivary gland function in patients with differentiated thyroid cancer treated with 131I
Authors: Cheng Yan , Tong Huimin , Li Xiaoqian , Zhang Xiaomin , Fang Ju , Yue Rongli , Huang Nan , Tian Jing , Yu Feiyan , Gao Ying 
Published: August 2022

Objective
The aim of this study was to evaluate the effect of vitamin E and supragingival scaling with vitamin C on the salivary glands of patients with differentiated thyroid carcinoma after 131I treatment.

Methods
A total of 89 prospective patients with differentiated thyroid carcinoma were enrolled and randomly divided into the following groups: vitamin E group (n = 30, group A), vitamin C group (n = 30, group B) and supragingival scaling with vitamin C group (n = 29, group C). Using functional indices (e.g. maximum uptake fraction, uptake index, excretion fraction, secretion time and excretion rate), changes in the salivary gland functions before and a month after 131I treatment were assessed by dynamic imaging of salivary gland.

Results
We compared the before and after 131I therapy results of the three groups. In group A (P  < 0.05), the excretion fraction and excretion rate of the left parotid gland were significantly higher, and the uptake index of the bilateral submandibular glands was significantly lower. No significant changes in salivary gland functional parameters were observed in group B (P > 0.05). The uptake index of the bilateral parotid glands and the excretion rate of the left parotid gland were significantly higher in group C (P < 0.05). The degree of serum amylase level reduction decreased significantly in group C (P < 0.05).

Conclusion
Vitamin E showed a protective effect on parotid excretion function in patients with differentiated thyroid carcinoma who underwent 131I treatment. Supragingival scaling may be a promising radiation protector because it is associated with a protective effect on the salivary gland functions.
Durable mechanical circulatory support as bridge to heart transplantation
Authors: Badiwala Mitesh , Dvirnik Nazari , Rao Vivek 
Published: August 2022

Purpose of review
Durable mechanical circulatory support (MCS) technology has changed over time as devices have evolved from pulsatile to continuous flow support. In this review, we discuss recent data and substantial changes to current practice as it pertains to the subject of current era durable left ventricular assist devices (LVADs) as a bridge to heart transplantation.

Recent findings
The results of heart transplantation in patients bridged with durable LVAD support are satisfactory even after prolonged duration of support. Reports of recent experience with LVAD related infection suggest that this complication has limited impact on post-transplant outcomes. Important sex-related disparities continue to exist following durable LVAD implantation. Recent changes in the United Network for Organ Sharing donor heart allocation policy have resulted in a drastic decline in the use of durable LVAD support for Bridge to Transplant in the United States.

Summary
Durable MCS in the form of LVAD as a BTT strategy continues to evolve over time. Optimization of its role in the treatment of end-stage heart failure, particularly in females will need to be the focus of future research.
Small fiber neuropathies: expanding their etiologies
Authors: Hoeijmakers Janneke G.J. , Merkies Ingemar S.J. , Faber Catharina G. 
Published: August 2022

Purpose of review
Several conditions have been associated with the development of small fiber neuropathy (SFN). The list of metabolic, immune-mediated, infectious, toxic, drugs-related, and hereditary conditions is still growing and various hypotheses are made about the underlying pathophysiological mechanisms. Understanding these processes is important to provide new targets for treatment. In addition, the specific SFN phenotype can provide direction for the underlying etiology. This review discusses the latest developments concerning the expanding etiologies in SFN.

Recent findings
In the past 18 months, special attention has been paid to immunological etiologies, partly due to the coronavirus disease 2019 pandemic, but also new auto-antibodies in SFN have been demonstrated. Identifying patients with immune-mediated SFN can be challenging, since contrary to the classical distal sensory phenotype, a nonlength-dependent pattern is more common.
Besides the etiologies of classical SFN, small fiber pathology is increasingly described in diseases without the typical neuropathic pain features of SFN, sometimes called syndromic SFN. However, the clinical relevance is not yet fully understood.

Summary
The expansion of the etiologies of SFN continues and brings more insight in possible targets for treatment. The clinical presentation may vary as a result of the underlying condition.
Sensory neuronopathies, diagnostic criteria and causes
Authors: Antoine Jean-Christophe 
Published: August 2022

Purpose of review
To stress on the diagnostic strategy of sensory neuronopathies (SNN), including new genes and antibodies.

Recent finding
SNN involve paraneoplastic, dysimmune, toxic, viral and genetic mechanisms. About one-third remains idiopathic. Recently, new antibodies and genes have reduced this proportion. Anti-FGFR3 and anti-AGO antibodies are not specific of SNN, although SNN is predominant and may occur with systemic autoimmune diseases. These antibodies are the only marker of an underlying dysimmune context in two-thirds (anti-FGFR3 antibodies) and one-third of the cases (anti-AGO antibodies), respectively. Patients with anti-AGO antibodies may improve with treatment, which is less clear with anti-FGFR3 antibodies. A biallelic expansion in the RFC1 gene is responsible for the cerebellar ataxia, neuropathy, vestibular areflexia syndrome (CANVAS) in which SNN is a predominant manifestation. Most of the patients have an adult onset and are sporadic. The RFC1 mutation may represent one-third of idiopathic sensory neuropathies. Finally, the criteria for the diagnosis of paraneoplastic SNN have recently been updated.

Summary
The diagnostic of SNN relies on criteria distinguishing SNN from other neuropathies. The strategy in search of their cause now needs to include these recent findings.
Identification of subgroups and development of prognostic risk models along the glycolysis-cholesterol synthesis axis in lung adenocarcinoma
Authors: Qian Bao , Guo Yangjie , Jiang Jiuzhou 
Published: August 2022

AbstractBackgroundLung cancer is one of the most dangerous malignant tumors affecting human health. Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer. Both glycolytic and cholesterogenic pathways play critical roles in metabolic adaptation to cancer.MethodsA dataset of 585 LUAD samples was downloaded from The Cancer Genome Atlas (TCGA) database. We obtained co-expressed glycolysis and cholesterogenesis genes by selecting and clustering genes from Molecular Signatures Database v7.5. Compared the prognosis of different subtypes and identified differentially expressed genes (DEGs) between subtypes. Predictive outcome events were modeled using machine learning, and the top 9 most important prognostic genes were selected by Shapley additive explanation (SHAP) analysis. A risk score model was built based on multivariate Cox analysis.ResultsLUAD patients were categorized into four metabolic subgroups: cholesterogenic, glycolytic, quiescent, and mixed. The worst prognosis was the mixed subtype. The prognostic model had great predictive performance in the test set.ConclusionsPatients with LUAD were effectively typed by glycolytic and cholesterogenic genes and were identified as having the worst prognosis in the glycolytic and cholesterogenic enriched gene groups. The prognostic model can provide an essential basis for clinicians to predict clinical outcomes for patients.
Meiotic drive adaptive testes enlargement during early development in the stalk-eyed fly
Authors: Bradshaw Sasha L , Meade Lara , Tarlton-Weatherall Jessica , Pomiankowski Andrew 
Published: August 2022

AbstractThe sex-ratio ‘SR’ X-linked meiotic drive system in stalk-eyed flies destroys all Y-bearing sperm. Unlike other SR systems, drive males do not suffer fertility loss. They have greatly enlarged testes, which compensate for gamete killing. We predicted that enlarged testes arise from extended development with resources re-allocated from the accessory glands, as these tend to be smaller in drive males. To test this, we tracked the growth of the testes and accessory glands of wildtype and drive males over 5–6 weeks post-eclosion before males attained sexual maturity. Neither of the original predictions are supported by this data. Instead, we found that the drive-male testes were enlarged at eclosion, reflecting a greater allocation of resources to the testes during pupation. In addition, there was no evidence that the greater allocation of resources to the testes during adult development retarded accessory gland growth. There was evidence of a general trade-off with eyespan, as males with larger relative eyespan had larger accessory glands but smaller testes. These findings support the idea that enlarged testes in drive males arise as an adaptive allocation of resources to traits that enhance male reproductive success.One sentence summaryAdaptive testes enlargement in early development ensures maintenance of fertility in stalk-eyed flies that lose half of their sperm due to meiotic drive
The Chromosome-based Genome of Paspalum vaginatum Provides New Insights into Salt-stress Adaptation
Authors: Liao Li , Hu Xu , Hao Jiangshan , Tang Minqiang , Ren Longzhou , Pan Ling , Xie Shangqian , Raymer Paul , Qi Peng , Chen Zhenbang , Wang Zhiyong , Luo Jie 
Published: August 2022

AbstractSalinization is increasingly a major factor limiting production worldwide. Revealing the mechanism of salt tolerance could help to create salt-tolerant crops and improve their yields. We reported a chromosome-scale genome sequence of the halophyte turfgrass Paspalum vaginatum, and provided structural evidence that it shared a common ancestor with Z. mays and S. bicolor. A total of 107 P. vaginatum germplasms were divided into two groups (China and foreign group) based on the re-sequenced data, and the grouping findings were consistent with the geographical origin. Genome-wide association study (GWAS) of visually scored wilting degree and withering rates identified highly significant QTL on chromosome 6. Combination with RNA-seq, we identified a significantly up-regulated gene under salt stress, which encodes ‘High-affinity K+ Transporter 7’ (PvHKT7), as strong candidates underlying the QTL. Overexpression of this gene in Arabidopsis thaliana significantly enhanced salt tolerance by increasing K+ absorption. This study adds new insights into salt-stress adaptation of P. vaginatum and serve as a resource for salt-tolerant improvement of grain crops.
Decoding the underlying neural activity of neurodegeneration in Alzheimer’s Disease
Authors: Li Haolin , Guo Hong 
Published: August 2022

SUMMARYAlzheimer’s Disease (AD) is the most prevalent chronic neurodegenerative disease, affecting more than 55 million people worldwide. AD is clinically characterized by a progressive loss of memory and other cognitive functions, which implicate the cholinergic system. Activity-induced neuroprotection based on gamma oscillation–audiovisual stimuli–is shown to be possible. Bulk RNA-seq data from treatment involving sensory stimuli were obtained, and bioinformatics analyses were performed. Activity-induced neuroprotection was found to be strongly associated with the downregulation of synaptic calcium signaling and cholinergic transmission. Candidate genes were screened in consultation with the Allen Brain Atlas Transcriptomics Explorer. We propose that Chrnb4 and Plcb4 are potential therapeutic targets for AD treatment. Chrnb4 has already been identified as a potential immune-relevant drug target gene using ontology inference, network analysis, and methylation signal, while multiple studies suggest that primary phospholipase C proteins play an integral role in inducing cell death in AD. Hence, both Chrnb4 and Plcb4 should be considered for future in vitro and in vivo studies as candidate genes.
Efficacy and feasibility of the minimal therapist-guided four-week online audio-based mindfulness program ‘Mindful Senses’ for burnout and stress reduction in medical personnel: A randomized controlled trial
Authors: Luangapichart Pavinee , Saisavoey Nattha , Viravan Natee 
Published: August 2022

AbstractBackgroundPrevious online mindfulness-based interventions (MBI) for burnout and stress reduction in medical personnel suffered from limited effect size and high dropout rate. The results were equivocal across studies with several limitations in their study designs. Therefore, we developed an online MBI entitled “Mindful Senses (MS)”, a minimally therapist-guided online audio-based mindfulness program, with aims to increase the effect size and lower dropout rate and tested its efficacy and feasibility with randomized active-controlled study design.MethodsWe recruited online 90 medical personnel who had moderate or high levels of burnout and stress from across Thailand. Participants were randomly allocated into Group A and B equally. Group A read four weekly Psychological Self-Help Articles (PSA) and attended the MS program during weeks 1-4. Group B read PSA during weeks 1-4 and attended MS program during week 9-12. Outcomes including burnout, stress, depression, anxiety, mindfulness, and quality of life (QOL) were measured at baseline (T0), week 4 (T1), and week 8 (T2) for both groups. Group X time interaction was analyzed by repeated-measures ANOVA.ResultsMS + PSA had significantly better improvement than PSA only in burnout, stress, anxiety, depression, mindfulness, and QOL, with medium-to-very large effect sizes (d = 1.33, 1.42, 1.04, 1.14, 0.70, and 1.03, respectively) at T1. The outcome differences remained at T2 with medium-to-large effect sizes (d = 0.84, 0.98, 0.73, 0.73, 0.66, and 0.94, respectively). The dropout rate was 4.4%.ConclusionsMS program is an online MBI that reduced burnout and stress in medical personnel with a large effect size and low dropout rate. Its effects remained at one-month follow-up. The MS program is feasible and has a potential to be an alternative intervention for medical personnel suffering from burnout and stress.Trial registrationthaiclinicaltrials.org TCTR20210416001
CRIC-seq reveals positional rule of PTBP1-mediated long-range RNA looping in splicing regulation
Authors: Ye Rong , Hu Naijing , Cao Changchang , Su Ruibao , Yang Chen , Xu Shihan , Xue Yuanchao 
Published: August 2022

SUMMARYRNA-binding proteins bind at different positions of pre-mRNA molecules to promote or reduce the usage of a particular exon. Seeking to understand the working principle of these positional effects, we develop a CRIC-seq method to enrich single RBP-mediated in situ RNA-RNA spatial interacting fragments for deep sequencing. We determine hnRNPA1- and PTBP1-mediated RNA-RNA interactions and regulatory mechanisms in HeLa cells. Unexpectedly, 3D RNA map analysis shows that PTBP1-mediated loops in introns preferably promote cassette exon splicing by accelerating asymmetric intron removal, whereas the loops spanning across cassette exon primarily repress splicing. This “positional rule” can faithfully predict PTBP1-regulated splicing outcomes. We further demonstrate that cancer-related splicing quantitative trait loci can disrupt RNA loops by reducing PTBP1 binding on pre-mRNAs to cause aberrant splicing in tumors. Our study presents a powerful method for exploring the functions of RBP-mediated RNA-RNA interactions in gene regulation and disease.
Chill injury in human kidney tubule cells after subzero storage is not mitigated by antifreeze protein addition
Authors: Tomalty Heather E. , Graham Laurie A. , Walker Virginia K. , Davies Peter L. 
Published: August 2022

AbstractBy preventing freezing, antifreeze proteins (AFPs) can permit cells and organs to be stored at subzero temperatures. As metabolic rates decrease with decreasing temperature, subzero static cold storage (SCS) could provide more time for tissue matching and potentially lead to fewer discarded organs. Human kidneys are generally stored for under 24 h and the tubule epithelium is known to be particularly sensitive to SCS. Here, telomerase-immortalized proximal-tubule epithelial cells from humans, which closely resemble their progenitors, were used as a proxy to assess the potential benefit of subzero SCS for kidneys. The effects of hyperactive AFPs from a beetle and Cryostasis Storage Solution were compared to University of Wisconsin Solution at standard SCS temperatures (4 °C) and at −6 °C for up to six days. Although the AFPs helped guard against freezing, lower storage temperatures under these conditions were not beneficial. Compared to cells at 4 °C, those stored at −6 °C showed decreased viability as well as increased lactate dehydrogenase release and apoptosis. This suggests that this kidney cell type might be prone to chilling injury and that the addition of AFPs to enable subzero storage may not be effective for increasing storage times.