bluuebunny/crossref_metadata_2025_split huggingface dataset:
Levels of whole salivary advanced glycation end products and interleukin-17 and peri-implant clinical and radiographic status in patients with osteoporosis at 6-years’ follow-up
Authors: Al-Qahtani Fawaz , Alqhtani Nasser , Divakar Darshan Devang , Alkhtani Fahad , Alqarawi Firas K. 
Published: November 2022

AbstractBackgroundThere are no studies that have assessed advanced glycation end products (AGEs) and interleukin 17A (IL-17A) levels in whole saliva (WS) of patients with dental implants. The aim was to compare levels of AGEs and IL-17A in WS and peri-implant clinical and radiographic status of patients with and without osteoporosis at 6-years’ follow-up.MethodsOsteoporotic (Group-1) and systemically healthy controls (Group-2) having undergone dental implant therapy at least 5 years ago were included. A questionnaire was used to collect information about age, gender, duration and treatment of osteoporosis, number and duration of implants in function, and frequencies of toothbrushing, flossing and hygiene visits. Modified bleeding and plaque indies (mBI and mPI), peri-implant probing depth (PD) and crestal bone loss (CBL) were recorded. WS was collected and levels of AGEs and IL-17A were determined using enzyme linked immunosorbent assay. Sample-size estimation was done and statistical analyses were doing using the independent t- and Wilcoxon rank-sum tests. Statistical significance was marked for P-values that were below 0.01.ResultsIn patients with (n = 24) and without (n = 27) osteoporosis, implants were in function for 6.3 ± 0.27 and 6.6 ± 0.5 years, respectively. There was no significant difference in peri-implant mBI, PD, mPI and CBL in both groups. Levels of AGE in patients with (119.6 ± 26.5 μg/ml) and without (91.5 ± 14.6 μg/ml) osteoporosis were comparable. Levels of whole salivary IL-17A in patients without and with osteoporosis were 4.6 ± 0.3 and 5.1 ± 0.8 pg/ml, respectively. Flossing of full mouth interproximal spaces once and twice daily toothbrushing daily was reported by 100% patients with osteoporosis. Among patients with osteoporosis 75% and 25% individuals were receiving professional dental prophylaxis every 4 and 6 months, respectively.ConclusionWithin the limitations of the present study osteoporotic patients are not at an increased risk of peri-implant diseases and can demonstrate salivary AGE and IL-17A levels comparable to non-osteoporotic individuals as long as oral hygiene is stringently maintained.
New Baitouweng decoction combined with fecal microbiota transplantation alleviates DSS-induced colitis in rats by regulating gut microbiota metabolic homeostasis and the STAT3/NF-κB signaling pathway
Authors: Gu Xin , Miao Zhiwei , Wang Yantian , Yang Yue , Yang Tongtong , Xu Yi 
Published: November 2022

AbstractAim of the studyWe aimed to elucidate the synergistic effect and potential mechanism of New Baitouweng Decoction (NBD) combined with fecal microbiota transplantation (FMT) in rats with DSS-induced ulcerative colitis (UC).Materials and methodsColitis was induced by 5% (w/v) dextran sulfate sodium (DSS) in drinking water for 7 days. NBD or NBD combined with FMT were administered to the colitis rats. Body weight and disease activity index were measured, and the colon histological change was imaged to further examine the efficacy of NBD and FMT. The specific effects of NBD on STAT3/NF-κB signaling pathway and gut microbiota in rats with UC were also investigated.ResultsThe efficacy of NBD in combination with FMT was demonstrated by the lower disease activity index scores; increased tight junction proteins expression; and a lower expression of macrophage marker (F4/80) in colon tissues. NBD combined with FMT elevated the concentrations of short-chain fatty acids and inhibited activation of the JAK2/STAT3/NF-κB related proteins. Furthermore, 16SrDNA sequencing indicated that the gut microbiota in rats with UC was perturbed, in contrast to that in healthy rats. After treatment with NBD and FMT, the diversity and abundance of intestinal flora showed clear improvements. Spearman correlation analysis indicated a strong correlation between specific microbiota and fecal concentrations of acetate, propionate and butyrate.ConclusionsThe protective mechanism of NBD combined with FMT may be linked to regulation NF-κB/STAT3 and restoration of the intestinal flora.
Novel localization of folate transport systems in the murine central nervous system
Authors: Sangha Vishal , Hoque Md. Tozammel , Henderson Jeffrey T. , Bendayan Reina 
Published: November 2022

Abstract
Background
Folates are a family of B9 vitamins that serve as one-carbon donors critical to biosynthetic processes required for the development and function of the central nervous system (CNS) in mammals. Folate transport is mediated by three highly specific systems: (1) folate receptor alpha (FRα; FOLR1/Folr1), (2) the reduced folate-carrier (RFC; SLC19A1/Slc19a1) and (3) the proton-coupled folate transporter (PCFT; SLC46A1/Slc46a1). Folate transport into and out of the CNS occurs at the blood–cerebrospinal fluid barrier (BCSFB), mediated by FRα and PCFT. Impairment of folate transport at the BCSFB results in cerebral folate deficiency in infants characterized by severe neurological deficiencies and seizures. In contrast to the BCSFB, CNS folate transport at other brain barriers and brain parenchymal cells has not been extensively investigated. The aim of this study is to characterize folate transport systems in the murine CNS at several known barriers encompassing the BCSFB, arachnoid barrier (AB), blood–brain barrier (BBB) and parenchymal cells (astrocytes, microglia, neurons).

Methods
Applying immunohistochemistry, localization of folate transport systems (RFC, PCFT, FRα) was examined at CNS barriers and parenchymal sites in wildtype (C57BL6/N) mice. Subcellular localization of the folate transport systems was further assessed in an in vitro model of the mouse AB. Gene and protein expression was analyzed in several in vitro models of brain barriers and parenchyma by qPCR and western blot analysis.

Results
RFC, PCFT, and FRα expression was localized within the BCSFB and BBB consistent with previous reports. Only RFC and PCFT expression was detected at the AB. Varied levels of RFC and PCFT expression were detected in neuronal and glial cells.

Conclusions
Localization of RFC and PCFT within the AB, described here for the first time, suggest that AB may contribute to folate transport between the peripheral circulation and the CSF. RFC and PCFT expression observed in astrocytes and microglia is consistent with the role that one or both of these transporters may play in delivering folates into cells within brain parenchyma. These studies provide insights into mechanisms of folate transport in the CNS and may enhance our understanding of the critical role folates play in neurodevelopment and in the development of novel treatment strategies for disorders of brain folate deficiency due to impaired transporter function.
First autochthonous clinical case of Hepatozoon silvestris in a domestic cat in Italy with unusual presentation
Authors: Simonato Giulia , Franco Vittoria , Salvatore Giovanna , Manzocchi Simone , Dotto Giorgia , Morelli Simone , Grillini Marika , Cavicchioli Laura , Gelain Maria Elena , Zini Eric 
Published: November 2022

AbstractHepatozoon spp. is the causative agent of a vector-borne parasitic disease in many animal species. In felids, Hepatozoon felis, Hepatozoon canis and Hepatozoon silvestris have been molecularly isolated. Hepatozoonosis usually causes asymptomatic infections in domestic cats, but clinical cases have recently been reported in Europe. We describe the first Italian case of hepatozoonosis in a cat with an unusual presentation. An 11-year-old neutered European shorthair cat was urgently hospitalized for intestinal intussusception. Hematology, biochemistry, FIV-FeLV tests, blood smears and molecular investigation targeting the 18S rRNA gene of Hepatozoon spp. were performed on blood samples; in addition, histological and molecular investigations were performed to analyze surgical samples to identify Hepatozoon infection. Hepatozoon gamonts were detected in granulocytes in the blood smear, and Hepatozoon spp. DNA was confirmed by PCR on blood. The intussusception was caused by a sessile endoluminal nodule that was surgically removed. Histologically, many elements referring to parasitic tissue forms were identified in the intestinal cells, and then the specimens were molecularly confirmed to harbor H. silvestris. This is the first description of symptomatic hepatozoonosis in a domestic cat in Italy. Hepatozoon silvestris has been described in wild felids, which are usually resilient to the infection, whereas the domestic cat seems to be more susceptible. Indeed, H. silvestris in cats usually presents tropism for skeletal muscle and myocardium with subsequent clinical manifestations. This is the first description of a domestic cat with H. silvestris localized in the intestinal epithelium and associated with intussusception.
Graphical abstract
Contribution of variant subunits and associated factors to genome-wide distribution and dynamics of cohesin
Authors: Cuadrado Ana , Giménez-Llorente Daniel , De Koninck Magali , Ruiz-Torres Miguel , Kojic Aleksandar , Rodríguez-Corsino Miriam , Losada Ana 
Published: November 2022

Abstract
Background
The cohesin complex organizes the genome-forming dynamic chromatin loops that impact on all DNA-mediated processes. There are two different cohesin complexes in vertebrate somatic cells, carrying the STAG1 or STAG2 subunit, and two versions of the regulatory subunit PDS5, PDS5A and PDS5B. Mice deficient for any of the variant subunits are embryonic lethal, which indicates that they are not functionally redundant. However, their specific behavior at the molecular level is not fully understood.

Results
The genome-wide distribution of cohesin provides important information with functional consequences. Here, we have characterized the distribution of cohesin subunits and regulators in mouse embryo fibroblasts (MEFs) either wild type or deficient for cohesin subunits and regulators by chromatin immunoprecipitation and deep sequencing. We identify non-CTCF cohesin-binding sites in addition to the commonly detected CTCF cohesin sites and show that cohesin-STAG2 is the preferred variant at these positions. Moreover, this complex has a more dynamic association with chromatin as judged by fluorescence recovery after photobleaching (FRAP), associates preferentially with WAPL and is more easily extracted from chromatin with salt than cohesin-STAG1. We observe that both PDS5A and PDS5B are exclusively located at cohesin-CTCF positions and that ablation of a single paralog has no noticeable consequences for cohesin distribution while double knocked out cells show decreased accumulation of cohesin at all its binding sites. With the exception of a fraction of cohesin positions in which we find binding of all regulators, including CTCF and WAPL, the presence of NIPBL and PDS5 is mutually exclusive, consistent with our immunoprecipitation analyses in mammalian cell extracts and previous results in yeast.

Conclusion
Our findings support the idea that non-CTCF cohesin-binding sites represent sites of cohesin loading or pausing and are preferentially occupied by the more dynamic cohesin-STAG2. PDS5 proteins redundantly contribute to arrest cohesin at CTCF sites, possibly by preventing binding of NIPBL, but are not essential for this arrest. These results add important insights towards understanding how cohesin regulates genome folding and the specific contributions of the different variants that coexist in the cell.
Ruptured innominate artery pseudoaneurysm presenting as hoarseness in Behçet’s syndrome: a case report
Authors: Johari Hamed Ghoddusi , Moein Seyed Arman , Shenavande Saeedeh , Amirian Armin , Nabavizadeh Sara Sadat 
Published: November 2022

Abstract
Background
Vascular involvement is an infrequent clinical manifestation of Behçet’s syndrome. Owing to the rarity of arterial involvement in Behçet’s syndrome, there is limited experience in managing this phenomenon.

Case presentation
Here, we report a 28-year-old Iranian man with a Behçet’s syndrome background, who presented with shoulder pain and hoarseness. Chest computed tomography angiography was conducted with a suspicion of a vascular pathology causing pressure on the recurrent laryngeal nerves. The patient was diagnosed with a ruptured innominate artery pseudoaneurysm. An innominate artery to the right common carotid artery bypass was performed, and the pseudoaneurysm was excised and replaced with an expandable polytetrafluoroethylene graft. Eventually, the patient was discharged after an uneventful hospital course.

Conclusion
It appears that we are still a long way from finding the optimal treatment for Behçet’s syndrome vascular involvement, and a combination of surgical and medicinal treatments is required.
O PAPEL DA EMBRAPA NAS TRANSFORMAÇÕES DO CERRADO
Authors: Franco José Benjamin Severino 
Published: November 2022

Through the reading of a suitable bibliography this work presents auspiciously in a
simplified way, as it was idealized and implanted the modernization of the agriculture in the countries in development (outlying) and for extension in Brazil. The countries that incorporated the idea of the "Green Revolutionâ€?, they took the responsibility of implanting it, creating for its end research centers. In Brazil EMBRAPA was created (Federal) and EMATER (State), both with the objective of disseminating the technological package contained in the "Green Revolution.â€?
The Society of Singularities—10 Theses
Authors: Reckwitz Andreas 
Published: November 2022

AbstractThe article summarizes the content of Andreas Reckwitz’s bookThe Society of Singularitiesin 10 theses and briefly links it to the author’s overall work.The Society of Singularitiesapplies a practice theory approach in order to outline a theory of Western (late-)modernity which recognizes in it a basic rivalry between two logics of social evaluation: a social logic of the general and a social logic of the particular/ singular. The question arises which historical causes for the surge of the social logic of uniqueness since the 1980s can be discerned, which structural features this type of society unfolds and which social and political consequences it has.
Social perception and support – An international sports event from the perspective of the residents of the host city
Authors: Máté Tünde 
Published: November 2022

AbstractWith the increase of international sports events in Hungary, their number, size, coverage, required investments, social impacts, the number of stakeholders, and people's involvement have also grown, while social support has bottomed out. How can we achieve social support? What are the factors that determine the perception of the residents of the organising city, thus, their social support? This question is answered by analysing the case of the European Youth Olympic Festival in Győr. The empirical research used quantitative methods, obtaining residents' opinions of international sports events before and after the event. The paper shows that a general positive opinion of international sports events is positively correlated with high levels of both spectator and participation sports consumption. Also, those who are personally satisfied with their quality of life generally support the organisation of international sports events and think positively of their impact. The level of satisfaction with life is correlated with satisfaction with the city and a positive opinion of its services. The regression model shows that personal involvement (e.g., interest, participation, and volunteering) is positively related to the evaluation of the impacts of sports events.
AVALIAÇÃO FÍSICO-QUÍMICA DE QUEIJO COALHO MATURADO E DEFUMADO PRODUZIDO COM OS LEITES DE CABRA
Authors: Alexandre Lignalva , Rufino José , Silva Rikelyne , Leite Mércia , Barbosa Neila 
Published: November 2022

O queijo coalho que está presente no dia-a-dia dos pernambucanos, é um produto obtido pela coagulação do leite bovino. Porém, atualmente, outras matrizes são utilizadas na sua produção como leites de cabra, ovelha e búfala. Como inovação, utilizou-se como matérias-primas para produção do queijo coalho maturado e defumado a mistura dos leites bovino e caprino. Este trabalho teve como objetivo inicial o desenvolvimento de um queijo do tipo coalho produzido com as matérias-primas leite bovino e leite caprino. O leite caprino utilizado para produção do queijo foi submetido à pasteurização lenta (65ºC por 30 minutos) e misturado com o leite bovino pasteurizado tipo A. Posteriormente adicionou-se o cloreto de cálcio, o coagulante e o sal. Depois de elaborado, o queijo foi maturado por 10 dias a uma temperatura de 12ºC e umidade relativa do ar de 95%, a defumação foi realizada em um defumador artesanal confeccionado a partir de latas de tinta reaproveitadas e utilizou-se a serragem como material de queima. Os processos de maturação e defumação do queijo conferiram características especiais ao produto como atenuações de sabor, odor, textura e consistência que vão se acentuando com o envelhecimento. Foram realizados ensaios físico-químicas para avaliar a qualidade do queijo produzido: pH, acidez titulável, lipídios, umidade, cinzas e proteínas). Baseado nos resultados físico-químicos, obteve-se 46,16% de umidade caracterizando o queijo de coalho produzido como queijo de alta umidade e 52,93% de gordura no extrato seco (GES) caracterizando-o como queijo gordo. Os valores médios para o pH compreenderam 5,73 a 6,18 e para o parâmetro de acidez titulável, entre 0,33 e 0,59g ácido lático/100g para um período de acompanhamento de seis semanas. O rendimento associado a produção do queijo maturado foi de 9,69L/Kg, após 10 dias de maturação o queijo perdeu cerca de 30% de sua massa inicial.
Appositions: The Future in Solarpunk and Post-Apocalyptic Fiction
Authors: Więckowska Katarzyna 
Published: November 2022

The essay discusses images of the future in solarpunk and post-apocalyptic fiction, focusing on their distinct approach to the narratives of progress, science, and individualism. The dystopian perspective of post-apocalyptic fiction is juxtaposed with the hopeful stance of solarpunk stories in order to outline the attempts to move beyond environmental pessimism and to imagine a liveable future. A reading of Cormac McCarthy’s The Road (2006), Erik M. Conway and Naomi Oreskes’s The Collapse of Western Civilization (2014), and Omar El Akkad’s American War (2017) provides an overview of early 21st-century dystopian motifs and visions, while the ideas and development of solarpunk fiction are discussed on the basis of three anthologies of short stories: Sunvault: Stories of Solarpunk and Ecospeculation (2017), Glass and Gardens: Solarpunk Summers (2018), and Multispecies Cities: Solarpunk Urban Futures (2021). The aim of the essay is to argue that apocalyptic and solarpunk fiction stand in a relationship of apposition to one another, representing dominant and emergent structures of feeling.
Performance of biological sand-fixing materials before and after inoculation on sandy desert surface
Authors: Tao Ling , Ren Hanru , Jun Ren 
Published: November 2022

Abstract
Aims Desert biocrust played an important role for the control of desertification. Artificial inoculation can promote the formation of biocrusts. The physiological and growth responses of the inoculated biological sand-fixing materials need to be clarified, and the results are of great significance for the artificial reproduction of desert biocrusts.
Methods The chlorophyll-a, thickness and fresh weight of biological sand-fixing materials surface were investigated in order to assess the effecting of mixture ratio, cultivation and inoculation times, and water supply under laboratory conditions.
Results The biological sand-sixing material mixed with cultivated algae crust and polymeric composites in 1:1 ratio demonstrated the best performance. Attapulgite clay could significantly promote the growth capacity and chlorophyll-a production of biological sand-fixing materials. The optimal biological sand-fixing material evidently accelerated the most accumulation of chlorophyll-a in 0.55 mg/kg, increase in 3.06 mm and growth of fresh biomass in 0.69 g/cm2, was the most beneficial to formation and development of artificial biocrust. The more water supply always significantly promoted the survival and growth of biological sand-fixing materials.
Conclusion The optimally proportioned biological sand-fixing material obviously promoted the most growth and accumulation of chlorophyll-a and biomass under artificial cultivation and inoculation treatments. The most water supply always produced the most chlorophyll-a, thickness and fresh weight, so incubating biocrusts always provided more production of chlorophyll-a, higher thickness and more biomass under more water supply.
Increased Expression of p-STAT3/IL-17 in Muscle Tissues of Patients with dermatomyositis and anti-synthetase syndrome Is Correlated with Disease Activity
Authors: Li Dongmei , Qin Kaixin , Hao Yiqun , Wang Kai , Luo DongPing , Yang Jie , Yang Bo , Jia Wen , Zhou Luyao , Fu Zili 
Published: November 2022

Abstract
Objective: To explore the roles of phosphorylated signal transduction and activator of transcription 3 (pSTAT3) and interleukin (IL)-17 expression of muscle in the disease activity of patients with idiopathic inflammatory myopathy (IIM).
Methods: Twenty-eight patients with IIM (20 with dermatomyositis [DM] and 8 with anti-synthetase syndrome [ASS]) and 12 healthy controls (HCs) were enrolled in the study. The expression levels of p-STAT3 and IL-17 in muscle tissues were examined using Western blotting and immunohistochemical analyses.
Results: The expressions of pSTAT3 were detected in atrophic and necrotic muscle cells around muscle fibers and in infiltrating inflammatory cells around blood vessels in DM patients and in mononuclear cells surrounding myocytes in ASS patients. The expressions of IL-17 were detected in muscle fibers and in infiltrating inflammatory cells around blood vessels in DM and ASS patients. However, they were not detected in the muscle tissue in HCs. Thus, the expression levels of p-STAT3 and IL-17 were significantly greater in the patients with IIM than in the HCs (P<0.05). Additionally, the p-STAT3 was positively correlated with the levels of IL-17in the muscle tissue ( r = 0.418, p = 0.027), IL-6 in the serum (r = 0.579, p = 0.001) , MYOACT score (r = 0.585, p = 0.001) , and the levels of AST (r =0.622, p < 0.001) , CK (r =0.714, p < 0.001) , LDH (r = 0.590, p = 0.001) , and HBD (r = 0.555, p = 0.003).
Conclusion: The STAT3/IL-17 pathway is over-activated in patients with PM/ASS and plays a significant role in the disease activity.