bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromoplast plastoglobules recruit the carotenoid biosynthetic pathway and contribute to carotenoid accumulation during tomato fruit maturation
Authors: Zita Wayne , Bressoud Ségolène , Glauser Gaetan , Kessler Felix , Shanmugabalaji Venkatasalam 
Published: September 2022

AbstractTomato (Solanum lycopersicum) fruit maturation is associated with a developmental transition from chloroplasts (in mature green fruit) to chromoplasts (in red fruit). The hallmark red color of ripe tomatoes is due to carotenogenesis and accumulation of the red carotenoid lycopene inside chromoplasts. Plastoglobules (PG) are lipid droplets in plastids that are involved in diverse lipid metabolic pathways. In tomato, information on the possible role of PG in carotogenesis and the PG proteome is largely lacking. Here, we outline the role of PG in carotenogenesis giving particular attention to tomato fruit PG proteomes and metabolomes. The proteome analysis revealed the presence of PG-typical FBNs, ABC1K-like kinases, and metabolic enzymes, and those were decreased in the PG of tomato chromoplasts compared to chloroplasts. Notably, the complete β-carotene biosynthesis pathway was recruited to chromoplast PG, and the enzymes PHYTOENE SYNTHASE 1 (PSY-1), PHYTOENE DESATURASE (PDS), ZETA-CAROTENE DESATURASE (ZDS), and CAROTENOID ISOMERASE (CRTISO) were enriched up to twelvefold compared to chloroplast PG. We profiled the carotenoid and prenyl lipid changes in PG during the chloroplast to chromoplast transition and demonstrated large increases of lycopene and β-carotene in chromoplast PG. The PG proteome and metabolome are subject to extensive remodeling resulting in high accumulation of lycopene during the chloroplast-to-chromoplast transition. Overall, the results indicate that PGs contribute to carotenoid accumulation during tomato fruit maturation and suggest that they do so by functioning as a biosynthetic platform for carotenogenesis.
Assessment of Variability in the Plasma 7k SomaScan Proteomics Assay
Authors: Candia Julián , Daya Gulzar N. , Tanaka Toshiko , Ferrucci Luigi , Walker Keenan A. 
Published: September 2022

ABSTRACTSomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7,288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2,050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.
Imprinted SARS-CoV-2 humoral immunity induces convergent Omicron RBD evolution
Authors: Cao Yunlong , Jian Fanchong , Wang Jing , Yu Yuanling , Song Weiliang , Yisimayi Ayijiang , Wang Jing , An Ran , Chen Xiaosu , Zhang Na , Wang Yao , Wang Peng , Zhao Lijuan , Sun Haiyan , Yu Lingling , Yang Sijie , Niu Xiao , Xiao Tianhe , Gu Qingqing , Shao Fei , Hao Xiaohua , Xu Yanli , Jin Ronghua , Shen Zhongyang , Wang Youchun , Xie Xiaoliang Sunney 
Published: September 2022

AbstractContinuous evolution of Omicron has led to a rapid and simultaneous emergence of numerous variants that display growth advantages over BA. 5. Despite their divergent evolutionary courses, mutations on their receptor-binding domain (RBD) converge on several hotspots. The driving force and destination of such convergent evolution and its impact on humoral immunity remain unclear. Here, we demonstrate that these convergent mutations can cause striking evasion of neutralizing antibody (NAb) drugs and convalescent plasma, including those from BA.5 breakthrough infection, while maintaining sufficient ACE2 binding capability. BQ.1.1.10, BA.4.6.3, XBB, and CH. 1.1 are the most antibody-evasive strain tested, even exceeding SARS-CoV-1 level. To delineate the origin of the convergent evolution, we determined the escape mutation profiles and neutralization activity of monoclonal antibodies (mAbs) isolated from BA.2 and BA.5 breakthrough-infection convalescents. Importantly, due to humoral immune imprinting, BA.2 and especially BA.5 breakthrough infection caused significant reductions in the epitope diversity of NAbs and increased proportion of non-neutralizing mAbs, which in turn concentrated humoral immune pressure and promoted convergent evolution. Moreover, we showed that the convergent RBD mutations could be accurately inferred by integrated deep mutational scanning (DMS) profiles, and the evolution trends of BA.2.75/BA.5 subvariants could be well-simulated through constructed convergent pseudovirus mutants. Together, our results suggest current herd immunity and BA.5 vaccine boosters may not provide good protection against infection. Broad-spectrum SARS-CoV-2 vaccines and NAb drugs development should be highly prioritized, and the constructed mutants could help to examine their effectiveness in advance.
Diverse branching forms regulated by a core auxin transport mechanism in plants
Authors: Spencer Victoria , Bentall Lucy , Harrison C. Jill 
Published: September 2022

AbstractDiverse branching forms have evolved multiple times across the tree of life to facilitate resource acquisition and exchange with the environment. In land plants, sporophyte branching enabled the diversification of the dominant vascular plant clade and arose in their last shared common ancestor; the bryophyte sisters to vascular plants are unbranched. Mechanisms for sporophyte branching are well known in Arabidopsis, where branch initiation and plastic branch outgrowth require directional auxin transport by PIN proteins. However, no broadly applicable genetic mechanisms for branching in vascular plants are known. We have used a combination of surgical and pharmacological treatments and PIN expression analyses in the lycophyte Selaginella kraussiana to identify PIN-mediated auxin transport as the ancestral mechanism for branching within vascular plants. We show that shortrange auxin transport out of the shoot tips promotes branching, and that branch dominance is coordinated by long-range auxin transport throughout the shoot system. Moreover, the plastic outgrowth of a branch from a unique organ system innovated in lycophytes (the rhizophore) is regulated by long-range auxin transport and associated with a transitory drop in PIN expression. We conclude that an ancestral mechanism for branching was independently recruited into plastic branch outgrowth in lycophytes and seed plants. Considered in conjunction with data from other species, our results highlight a pivotal role for the co-option of PINs into the evolution of branching in diverse plant forms.
Misexpression of the Homologues of Bryophyte Gametophyte-to-Sporophyte Control Genes in Arabidopsis Results in Germline Reprogramming and Phenotypes that Mirror Apomictic Development
Authors: Bezodis William , Prescott Helen , Dickinson Hugh 
Published: September 2022

AbstractEvidence from the model bryophytes Physcomitrium and Marchantia suggests that a BELL-KNOX genetic module acts as a master regulator controlling sporophyte identity. Investigating any conservation of this system in flowering plants has proved challenging, but studies of the Arabidopsis eostre mutant and naturally apomictic angiosperms point to ectopic activation of KNOX and BELL transcription factors mediating the switch from sexual to apomictic development. We show here that in Arabidopsis, ectopic expression, under a germline-specific promoter, of KNOX and BELL genes not normally expressed in the gametophytes both disrupts germ cell specification and causes defects in cell identity throughout gametophyte development – some mirroring events seen in naturally apomictic plants. A better understanding of this TALE-HD genetic module in flowering plants may thus help to unravel the molecular control of higher plant life cycles, while providing a route to engineering synthetic apomixis in crops. This study also highlights the utility of applying data from bryophytes, where the ontogeny transitions are spatio-temporally distinct, to apomixis research in angiosperms.
MTCH2 is a mitochondrial outer membrane protein insertase
Authors: Guna Alina , Stevens Taylor A. , Inglis Alison J. , Replogle Joseph M. , Esantsi Theodore K. , Muthukumar Gayathri , Shaffer Kelly C.L. , Wang Maxine L. , Pogson Angela N. , Jones Jeff J. , Lomenick Brett , Chou Tsui-Fen , Weissman Jonathan S. , Voorhees Rebecca M. 
Published: September 2022

AbstractIn the mitochondrial outer membrane, tail-anchored (TA) proteins play critical roles in cytoplasmic-mitochondrial communication. Using genome-wide CRISPRi screens, we identify factors involved in mitochondrial TA biogenesis in human cells. We show that MTCH2, and its paralog MTCH1, are required for insertion of biophysically diverse mitochondrial TAs, but not outer membrane β-barrel proteins. In a reconstituted system, purified MTCH2 is sufficient to mediate insertion into proteoliposomes. Functional and mutational studies reveal that MTCH2 uses membrane-embedded hydrophilic residues to function as a gatekeeper for outer membrane protein biogenesis, controlling mislocalization of TAs into the endoplasmic reticulum and the sensitivity of leukemia cells to apoptosis. Our identification of MTCH2 as an insertase provides a mechanistic explanation for the diverse phenotypes and disease states associated with MTCH2 dysfunction.One-Sentence SummaryMTCH2 is both necessary and sufficient for insertion of diverse α-helical proteins into the mitochondrial outer membrane, and is the defining member of a family of insertases that have co-opted the SLC25 transporter fold.
A novel gyrovirus associated with a fatal respiratory disease in yellow-eyed penguin (Megadyptes antipodes) chicks
Authors: Wierenga Janelle R. , Morgan Kerri J. , Hunter Stuart , Taylor Harry S. , Argilla Lisa S. , Webster Trudi , Dubrulle Jeremy , Jorge Fátima , Bostina Mihnea , Burga Laura , Holmes Edward C. , McInnes Kate , Geoghegan Jemma L. 
Published: September 2022

AbstractYellow-eyed penguins (Megadyptes antipodes), or hoiho in te reo Māori, are predicted to become extinct on mainland Aotearoa New Zealand in the next few decades, with infectious disease a significant contributor to their decline. A recent disease phenomenon termed respiratory distress syndrome (RDS) causing lung pathology has been identified in very young chicks. To date, no causative pathogens for RDS have been identified. In 2020 and 2021, the number of chick deaths from suspected RDS increased four- and five-fold, respectively, with a mortality rate of >90%. Here, we aimed to identify possible pathogens responsible for RDS disease impacting yelloweyed penguins. Total RNA was extracted from tissue samples collected during post-mortem of 43 chicks and subject to metatranscriptomic sequencing. From these data we identified a novel and highly abundant gyrovirus in 80% of tissue samples. This virus exhibited only 41% amino acid identity within VP1 to its closest relative, Gyrovirus 8, discovered in a diseased seabird. No other exogenous viral transcripts, nor pathogenic bacterial, protozoal and fungal organisms, were identified in these tissues. Due to the high relative abundance of viral reads, it is likely that this novel gyrovirus is associated with RDS in yellow-eyed penguin chicks.Author SummaryNew Zealand’s population of yellow-eyed penguins, also called hoiho, are predicted to become extinct in the next 20-30 years, with disease a major factor contributing to their decline. A new disease, causing fluid and bleeding into the lungs, was initially identified in 2019 in very young chicks. It was characterised as causing respiratory distress with a mortality of >90% usually within the first week of life. To date, no causative pathogens of the disease have been identified. We aimed to identify possible pathogens responsible for respiratory disease in these penguin chicks. A metatranscriptomic survey of dead chicks identified a novel and highly abundant gyrovirus present in diseased tissue, with closely related viruses causing disease in other avian hosts. It is, therefore, highly likely that this novel gyrovirus is associated with respiratory disease in these chicks. This finding offers the potential to increase the success of disease management in the critically endangered yellow-eyed penguin and possibly other at-risk penguin species. The potential to lessen mortality and slow the decline of the species is essential in protecting the biodiversity of New Zealand’s fauna and flora.
Alpha-T-catenin is expressed in peripheral nerves as a constituent of Schwann cell adherens junctions
Authors: Weng Anthea , Rabin Erik E. , Flozak Annette S. , Chiarella Sergio E. , Aillon Raul Piseaux , Gottardi Cara J. 
Published: September 2022

ABSTRACTThe adherens junction component, alpha-T-catenin (αTcat) is an established contributor to cardiomyocyte junction structure and function, but recent genomic studies linkCTNNA3 polymorphismsto diseases with no clear cardiac underpinning, including asthma, autism and multiple sclerosis, suggesting causal contributions from a different cell-type. We showCtnna3mRNA is highly expressed in peripheral nerves (e.g., vagus and sciatic), where αTcat protein enriches at paranodes and myelin incisure adherens junctions of Schwann cells. We validate αTcat immunodetection specificity using a newCtnna3-knockout fluorescence reporter mouse line yet find no obvious Schwann cell loss-of-function morphology at the light microscopic level.CTNNA3/Ctnna3mRNA is also abundantly detected in oligodendrocytes of the central nervous system via public databases, supporting a general role for αTcat in these unique cell-cell junctions. These data suggest that the wide range of diseases linked toCTNNA3may be through its role in maintaining neuroglial functions of central and peripheral nervous systems.
Do accounting disclosures help or hinder individual donors’ trust repair after negative events?
Authors: Guo Zhengqi , Hall Matthew , Wiegmann Leona 
Published: September 2022

PurposeThis study aims to examine whether and how voluntary accounting disclosures can repair individual donors’ trust in a charity after negative events.Design/methodology/approachThe authors adopt a qualitative research approach and conduct 32 semi-structured interviews with active Australian individual donors, with a hypothetical vignette design. Hypothetical negative events and corresponding accounting disclosures are presented to participants during interviews.FindingsThree types of individual donors are identified based on their decision-making patterns after negative events and primary trust relations with a charity-reasoned donor (giving-decision based on their analysis of the situation, competence-based trust), generalist donors (giving-decision based on trust in the charitable sector, institution-based trust) and emotional donors (giving-decision based on feelings and emotions about the charity, integrity-based trust). The research suggests that accounting disclosures can repair trust damage for reasoned donors and support institution-based trust for generalist donors, but do not seem able to repair trust damage for emotional donors and can potentially damage trust further.Practical implicationsOverall, the findings suggest that a one-size-fits-all approach to communicating with individual donors after negative events is not likely to be very effective in repairing trust. Instead, charities may need to adapt disclosures to their different types of individual donors.Originality/valueWhile prior accounting studies have largely focussed on how charity managers themselves grapple with accountability or how negative events impact charitable donations, the authors demonstrate how accounting disclosures can play different roles in the trust-repairing process for different types of individual donors.
Artificial intelligence in retail – a systematic literature review
Authors: Heins Caroline 
Published: September 2022

PurposeThe purpose of this study is to present a systematic literature review of academic peer-reviewed articles in English published between 2005 and 2021. The articles were reviewed based on the following features: research topic, conceptual and theoretical characterization, artificial intelligence (AI) methods and techniques.Design/methodology/approachThis study examines the extent to which AI features within academic research in retail industry and aims to consolidate existing knowledge, analyse the development on this topic, clarify key trends and highlight gaps in the scientific literature concerning the role of AI in retail.FindingsThe findings of this study indicate an increase in AI literature within the field of retailing in the past five years. However, this research field is fairly fragmented in scope and limited in methodologies, and it has several gaps. On the basis of a structured topic allocation, a total of eight priority topics were identified and highlighted that (1) optimizing the retail value chain and (2) improving customer expectations with the help of AI are key topics in published research in this field.Research limitations/implicationsThis study is based on academic peer-reviewed articles published before July 2021; hence, scientific outputs published after the moment of writing have not been included.Originality/valueThis study contributes to the in-depth and systematic exploration of the extent to which retail scholars are aware of and working on AI. To the best of the author’s knowledge, this study is the first systematic literature review within retailing research dealing with AI technology.
Is eco-label hotel engagement the pathway to sustainability practices via entrepreneurial resilience and orientation in Oman? Findings from PLS-SEM and fsQCA
Authors: Salem Islam Elbayoumi , Elbaz Ahmed Mohamed , Al-alawi Alamir , Alkathiri Nasser Alhamar , Elkhwesky Zakaria 
Published: September 2022

Purpose
This study aims to examine the role of eco-label hotel engagement as a pathway to sustainable practices via scouting entrepreneurial resilience and orientation at highly ranked hotels in Oman. The authors developed and tested a novel model built on resilience theory, the theory of entrepreneurial orientation and the theory of reasoned action (TRA).

Design/methodology/approach
Data from 167 human resources directors, hotel managers and other employees were analyzed by partial least squares structural equation modeling (PLS-SEM) and fuzzy-set qualitative comparative analysis (fsQCA).

Findings
Results indicate a strong positive relationship between entrepreneurial resilience and entrepreneurial orientation. Entrepreneurial orientation significantly increased all eco-label strategies, but not all strategies enhanced sustainable tourism practices. High adoption of sustainability practices depended on core strategies related to awareness, benefits, reputation and necessity, but the cost was also an issue. Managers adopted sustainability practices if they were not perceived as costly, or when perceived as costly if they believed they would help them reduce operating costs.

Practical implications
Policymakers should assist hotel managers when the sector is hit by political events, natural disasters or health crises such as the current pandemic can bounce back and develop their resilience. Likewise, training and workshops can be organized to improve managers’ entrepreneurial mindset, which was found to be a precursor to favorable attitudes toward sustainability.

Originality/value
This study tests a novel model built on three theories: resilience theory, the theory of entrepreneurial orientation and the TRA by using PLS-SEM and fsQCA.
Effects of customers’ perceived risks of sharing economy on self-protective behavior toward COVID-19
Authors: Hailey Shin Hyejo , Jeong Miyoung , Zapata-Cuervo Natalia , Montes Guerra Maricela Isabel , Cho Mi-Hea , Kim Yensoon 
Published: September 2022

Purpose
This study aims to investigate how customers’ perceived risks of sharing economy (SE) affect their self-protective behaviors when using SE, leading to their future behavioral intention. Additionally, this study looks into whether there are any differences between accommodation-sharing and ride-sharing customers in the aforementioned relationships.

Design/methodology/approach
An online survey targeting two groups of SE customers (i.e. accommodation sharing and ride sharing) was used. Using partial least squares structural equation modeling, the mechanism of how SE customers’ perceived risks of SE affect their self-protective behaviors, which in turn influence their future behavior intention. A multigroup analysis was performed to assess the difference between the two groups of SE customers. Finally, a multivariate analysis of variance (MANOVA) was conducted to see the potential differences between the five classifications of self-protective behaviors in their perceived risks.

Findings
SE customers’ psychological risks positively affected their hygiene protective behaviors and social protective behaviors, influencing their behavior intention and relative intention (compared with traditional services). Social risk had a negative impact on SE customers’ hygiene protective behaviors. There was a significant difference between accommodation sharing and ride sharing customers in their psychological mechanism of how perceived risks influence their self-protective behaviors.

Practical implications
The findings of this study help SE platforms and service providers better understand their customers’ perceived risks of their services and suggest them to promote their customers’ self-protective behaviors so that perceived risks can be mitigated, thereby generating strong behavior intentions. As the results indicated that there is a significant difference between the two major forms of SE (i.e. accommodation sharing and ride sharing) in their customers’ perceived risks and self-protective behavior, SE platforms can further refine their operational and marketing efforts based on the findings.

Originality/value
This study offers a comprehensive understanding of SE customers’ self-protective behaviors by examining the effects of SE customers’ different perceived risks on their self-protective behaviors during the unprecedented pandemic. Furthermore, the comparison of the two most popular forms of SE (i.e. accommodation sharing and ride sharing) provides new perspectives to understand customers’ behavior in the SE context.