bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression And Clinical Significance of Transcription Factor GRHL1 In Acute Myeloid Leukemia
Authors: Hua Jing , Ma Congcong , Wang Zhaohui , Duan Xianliang , Zhang Xiaole , Bi Yazhen , Wang Yan , Xiao Taiwu , Zhu Chuansheng 
Published: January 2022

Abstract
Background: Acute myeloid leukemia (AML) ranks sixth in the incidence of malignant tumors. It is the most common acute leukemia in adults with clinical and molecular heterogeneity of the bone marrow and lymphatic system.Objective: In this study, we aimed to explore the expression and clinical significance of GRHL1 in acute myeloid leukemia.Methods: RNA-seq data from The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx) and Gene Expression Omnibus (GEO) were analyzed combined with the related clinical data of patients with AML. Kaplan–Meier plot was constructed to assess survival differences. Log-rank test and correlation analysis were performed to evaluate the prognostic value and clinical significance of GRHL1 in patients with AML. Results: In this study, we found that patients with AML showed significant differences in the GRHL1 gene expression compared to normal bone marrow controls. Kaplan-Meier survival analysis revealed that low expression of GRHL1 was significantly associated with worse prognosis in patients with AML. Multiple omics analysis and correlation analysis revealed that aberrant alterations of known factors were significantly associated with GRHL1 expression, which confirmed GRHL1 as a protective intervention factor in patients with AML.Discussion: GRHL1 presented great potential in evaluating predicting prognostic values and clinical significance in patients with AML. GRHL1 might act as an important role in inhibiting the progression of tumors and serve as a protective factor for potential therapeutic strategies of AML. GRHL1 might provide a novel biomarker for precision oncology and benefit clinical cancer management.
Comparative Genomics of Bacillus Subtilis MZK05 and Its Mutant Strain Revealed Genetic Factors Responsible for Enhanced Serine Protease Expression
Authors: Hoq Md. Mozammel , Hasan Md. Mahmud , Karim Muhammad Manjurul , Mamun Md. Arafat Al , Khan Shakila Nargis 
Published: January 2022

Abstract
Genome sequence study of an industrially-important strain, Bacillus subtilis M9, a mutant version of wild strain Bacillus subtilis MZK05 was conducted to uncover genetic factors responsible for enhanced serine proteases expression in addition to its other industrial enzymes, metabolites and bacteriocins producing efficacy. The wild type and the mutant genome contained a size of 4,145,727 and 4,045,950 bp, with 4,352 and 4,383 genes; and 477 and 478 subsystems respectively. Genomic comparison with 31 B. subtilis sourced from different countries showed both wild and mutant shared same type of genome structure with 20 others. Moreover, 6,000 kb pangenome showed that they share 3082, 1449, and 25757 core, unique and accessory genes respectively. A sum of 32,559 mutations were found with three major genomic structural changes in the upstream and downstream of an extracellular alkaline serine protease, AprX and a periplasmic serine protease, HtrC in M9 genome when compared to the wild type. Furthermore, 11 different serine protease genes and 4 different signal peptidases were found with several mutational changes in M9. In addition, mutations found in core genome of BsM9 in phage-like element, major capsid protein, and phage portal protein are the likely reasons of high-level serine protease activity.
Impact of Corona Pandemic On Healthcare System: a Case Study of Raxaul Town
Authors: Gupta Deepali , Kumar Pankaj 
Published: January 2022

Abstract
The primary health care system is the most protruding health system in any nation's health index measures. The worthiness is tested at times when the entire nation is fighting against a global epidemic that is the result of any biological disaster that occurred in any part of the world and has spread across their geographical boundary. The PHCs then becomes the focal point for the entire human community. Likewise, in the present time, the entire world is struggling with the corona pandemic. Scientists and medical practitioners from across the globe are working day and night, experimenting and researching to find a cure to this disaster which spread to parts of the world from a biological lab of Wuhan, China. Since the entire nation has been affected and many hospitals and nursing centres have had challenges with the current situations, it becomes important to examine the impact of the coronavirus pandemic on the primary health care system with special reference to the city of Raxaul, Bihar. It is very important to scientifically study and analyze the primary and secondary data collected for this research paper. The sources of data collection being the various daily newspaper articles, magazines, personal interviews with staff of the government and non-governmental organizations, public, administration and police personals. The data has been collected using random sampling and personal interview techniques. After carefully examining the results of the data collected, we can conclude the effect of the corona pandemic can be widely seen on the primary health care system, which proves the absence of basic facilities, structural anomalies and healthcare staff availability.
Regulation of Key Enzymes in Lentinula Edodes Mycelium Culture by Chitosan
Authors: Yan Xunyou , Shi Zhenxia , Chen Wenjie , Ma Sijia , Wang Fangfang , Ren Sizhu 
Published: January 2022

Abstract
Chitosan is a plant growth regulator. Since it has an important function, it may also play a similar role in the growth of fungi. In this study, Lentinula edodes was used as a model fungus, and the mycelium growth and metabolism in the cultivation bag treated with different concentrations of chitosan were investigated. The results showed when the chitosan concentration was 0.1 mg/ml, a dense mycelium was developed and the fast growth was observed. Compared with other concentrations and the control group, they had significant differences. Furthermore, the activity of CMCase, Amy, and POD were measured, which showed significant differences in the rapid growth of the mycelium compared with other concentrations. The concentrations of 0.1 mg/mL will be chosen to promote the growth of Lentinula edodes' mycelium in practical production.
Manipulation of Rotational States With Varying Pulse Parameter: Achieving High Molecular Alignment and Orientation for CH3F
Authors: Chordiya Kalyani , Simkó Irén , Szidarovszky Tamás , Kahaly Mousumi Upadhyay 
Published: January 2022

Abstract
Increasing interest in the fields of high-harmonics generation, laser-induced chemical reactions, and molecular imaging of gaseous targets demands high molecular alignment and orientation (AnO). In this work, we examine the critical role of different pulse parameters on the field-free AnO dynamics of the CH3F molecule, and identify experimentally feasible optical and THz range laser parameters that ensure maximal AnO for such molecules. Herein, apart from rotational temperature, we investigate effects of varying pulse parameters such as, pulse duration, intensity, frequency, and carrier envelop phase (CEP). By analyzing the interplay between laser pulse parameters and the resulting rotational population distributions, the origin of specific AnO dynamics was addressed. We could identify two qualitatively different AnO behaviors and revealed their connection with the pulse parameters and the population of excited rotational states. We report here the highest alignment of ⟨cos2θ⟩=0.843 andorientation of⟨cos(θ)⟩=0.886 for CH3F molecule at 2K using single pulse. Our study should be useful to understand different aspects of laser-induced unidirectional rotation in heteronuclear molecules, and in understanding routes to tune/enhance AnO in laboratory conditions for advanced applications.
FIR Digital Filter Design Based on Improved Artificial Bee Colony Algorithm
Authors: Lian Lian , Tian Zhongda 
Published: January 2022

Abstract
The traditional swarm intelligence optimization algorithm is prone to fall into local optimal solutions in finite impulse response (FIR) digital filter design, and has slow convergence speed. In order to optimize the design of FIR filter, a FIR digital filter design method based on improved bee colony (ABC) algorithm is proposed. This improved ABC algorithm can adaptively adjust the step size of the selected neighborhood of nectar source location. At the same time, the information of the global optimal solution is used to guide the search of candidate solution, which improves the global search ability of the algorithm. The improved ABC algorithm can balance the conflict between local search ability and global search ability, so it can achieve better optimization effect. The time and space complexity of the algorithm is analyzed in detail. Then, the improved ABC algorithm is used to design three typical FIR digital filters, namely low-pass, band-pass and band-stop filter. The performance of the designed filter is tested by simulation experiments. The experimental results show that compared with other state-of-the-art optimization algorithms, the proposed FIR filter design method has achieved better effect and performance. Meanwhile, the proposed design method has shorter optimization time. The superiority of the proposed method is verified.
A Large-Scale Sentiment Analysis of Tweets Pertaining To The 2020 US Presidential Election
Authors: Ali Rao Hamza , Pinto Gabriela , Lawrie Evelyn , Linstead Erik J 
Published: January 2022

Abstract
We capture the public sentiment towards candidates in the 2020 US Presidential Elections, by analyzing 7.6 million tweets sent out between October 31st and November 9th, 2020. We apply a novel approach to first identify tweets and user accounts in our database that were later deleted or suspended from Twitter. We present sentiment analysis of the presidential candidates across these identified posts alongside active ones, and share key insights. The aim is to highlight the importance of conducting sentiment analysis on all posts captured in real time, including those that are now inaccessible, in determining the true sentiments of the opinions around the time of an event.
5’isomiR-183-5p|+2 Elicits Tumor Suppressor Activity in a Negative Feedback Loop with E2F1
Authors: Li Xiaoya , Tosun Oyku Ece , Jung Janine , Kappes Jolane , Ibing Susanne , Nataraj Nishanth Belugali , Sahay Shashwat , Schneider Martin , Wörner Angelika , Becki Corinna , Ishaque Naveed , Feuerbach Lars , Michels Birgitta Elisabeth , Heßling Bernd , Helm Dominic , Will Rainer , Yarden Yosef , Müller-Decker Karin , Körner Cindy , Wiemann Stefan 
Published: January 2022

Abstract
Background: MicroRNAs (miRNAs) and isomiRs play important roles in tumorigenesis as essential regulators of gene expression. 5’isomiRs exhibit a shifted seed sequence compared to the canonical miRNA, resulting in different target spectra and thereby extending the phenotypic impact of the respective common pre-miRNA. However, for most miRNAs, expression and function of 5’isomiRs have not been studied in detail yet. Therefore, this study aims to investigate the functions of miRNAs and their 5’isomiRs.Methods: The expression of 5’isomiRs was assessed in The Cancer Genome Atlas (TCGA) breast cancer patient dataset. Phenotypic effects of miR-183 overexpression in triple-negative breast cancer (TNBC) cell lines were investigated in vitro and in vivo by quantifying migration, proliferation, tumor growth and metastasis. Direct targeting of E2F1 by miR-183-5p|+2 was validated with a 3’UTR luciferase assay and linked to the phenotypes of isomiR overexpression.Results: TCGA breast cancer patient data indicated that three variants of miR-183-5p are highly expressed and upregulated, namely miR-183-5p|0, miR-183-5p|+1 and miR-183-5p|+2. However, TNBC cell lines displayed reduced proliferation and invasion upon overexpression of pre-miR-183. While invasion was reduced individually by all three isomiRs, proliferation and cell cycle progression were specifically inhibited by overexpression of miR-183-5p|+2. Proteomic analysis revealed reduced expression of E2F target genes upon overexpression of this isomiR, which could be attributed to direct targeting of E2F1, specifically by miR-183-5p|+2. Knockdown of E2F1 partially phenocopied the effect of miR-183-5p|+2 overexpression on cell proliferation and cell cycle. Gene set enrichment analysis of TCGA and METABRIC patient data indicated that the activity of E2F strongly correlated with the expression of miR-183-5p, suggesting transcriptional regulation of the miRNA by a factor of the E2F family. Indeed, in vitro, expression of miR-183-5p was regulated by E2F1. Hence, miR-183-5p|+2 directly targeting E2F1 appears to be part of a negative feedback loop potentially fine-tuning its activity.Conclusions: This study demonstrates that 5’isomiRs originating from the same arm of the same pre-miRNA (i.e. pre-miR-183-5p) may exhibit different functions and thereby collectively contribute to the same phenotype. Here, one of three isomiRs was shown to counteract expression of the pre-miRNA by negatively regulating a transcriptional activator (i.e. E2F1). We speculate that this might be part of a regulatory mechanism to prevent uncontrolled cell proliferation, which is disabled during cancer progression.
Association Between Coronary Artery Disease And Clinical Outcome In Cancer Patients: A Propensity Score Matching Analysis
Authors: Huang Zengfa , Huang Zheng , Cao Beibei , Li Zuoqin , Yang Yang , Ding Yi , Zhao Shengchao , Hu Yunting , Sun Ruihong , Wang Zheng , Zhang Hongfeng , Wang Xiang 
Published: January 2022

Abstract
The aim of this study was to evaluate the prognostic value of coronary computed tomography angiography (CTA) to predict the risk of all-cause mortality in cancer patients in a propensity score matching (PSM) analysis. A total of 331 patients who previously had cancer and underwent coronary CTA from January 2015 to December 2019 were included. Multivariate Cox proportional hazards regression analysis and propensity-score matching analysis were performed. The primary endpoint was all-cause of mortality. In total, 125 with obstructive coronary artery disease (CAD) and 206 with non-obstructive CAD during a median follow-up of 3.3 years were included in this study. After PSM, age (HR, 1.040; 95%CI, 1.001-1.081; p =0.046), smoking history (HR, 2.344; 95%CI, 1.211-4.536; p =0.011), diabetes mellitus (HR, 1.862; 95%CI, 1.053-3.292; p =0.033) and obstructive CAD (HR, 1.845; 95%CI, 1.008-3.377; p =0.047) remained significant factors for all-cause mortality. Obstructive CAD evaluated by coronary CTA was found to be at higher risk for developing all-cause mortality in cancer patients.
Targeting Lactate Dehydrogenase B-dependent Mitochondrial Metabolism Affects Tumor Initiating Cells and Inhibits Tumorigenesis of Non-small Cell Lung Cancer by Inducing MtDNA Damage
Authors: Marti Thomas Michael , Deng Haibin , Gao Yanyun , Trappetti Verdiana , Hertig Damian , Karatkevich Darya , Losmanova Tereza , Urzi Christian , Ge Huixiang , Geest Gerrit Adriaan , Bruggmann Remy , Djonov Valentin , Nuoffer Jean-Marc , Vermathen Peter , Zamboni Nicola , Riether Carsten , Ochsenbein Adrian , Peng Ren-Wang , Kocher Gregor , Schmid Ralph Alexander , Dorn Patrick 
Published: January 2022

Abstract
Once considered a waste product of anaerobic cellular metabolism, lactate has been identified as a critical regulator of tumorigenesis, maintenance, and progression. The putative primary function of lactate dehydrogenase B (LDHB) is to catalyze the conversion of lactate to pyruvate; however, its role in regulating metabolism during tumorigenesis is largely unknown. To determine whether LDHB plays a pivotal role in tumorigenesis, we performed 2D and 3D in vitro experiments, utilized a conventional xenograft tumor model, and developed a novel genetically engineered mouse model (GEMM) of non-small cell lung cancer (NSCLC), in which we combined an LDHB deletion allele with an inducible model of lung adenocarcinoma driven by the concomitant loss of p53 (also known as Trp53) and expression of oncogenic KRAS (G12D) mutant (KP). Here, we show that epithelial-like, tumor-initiating NSCLC cells feature oxidative phosphorylation (OXPHOS) phenotype that is regulated by LDHB-mediated lactate metabolism. We show that silencing of LDHB induces persistent mitochondrial DNA damage, decreases mitochondrial respiratory complex activity and OXPHOS, resulting in reduced levels of mitochondria-dependent metabolites, e.g., TCA intermediates, amino acids, and nucleotides. In addition, inhibition of LDHB dramatically reduced the survival of tumor-initiating cells and sphere formation in vitro, which can be partially restored by nucleotide supplementation. In addition, LDHB silencing reduced tumor initiation and growth of xenograft tumors. Furthermore, we report for the first time that homozygous deletion of LDHB significantly reduced lung tumorigenesis upon the concomitant loss of Tp53 and expression of oncogenic KRAS without considerably affecting the animal’s health status, thereby identifying LDHB as a potential target for NSCLC therapy. In conclusion, our study shows for the first time that LDHB is essential for the maintenance of mitochondrial metabolism, especially nucleotide metabolism, demonstrating that LDHB is crucial for the survival and proliferation of tumor-initiating cells and tumorigenesis.
Effect of Combined Wet Alkaline Mechanical Pretreatment on Enzymatic Hydrolysis of Corn Stover and Its Mechanism
Authors: Shi Suan , Yang Jie , Gao Chongfeng , Yang Xueqi , Su Yanfu , Han Lujia 
Published: January 2022

Abstract
Background: To further optimize the mechanochemical pretreatment process, a combined wet alkaline mechanical pretreatment of corn stover at room temperature was proposed. Results: The combined alkaline mechanical pretreatment significantly enhanced enzymatic hydrolysis resulting in a glucose yield (YG) of 491.9mg/g (91.9%) with 3% NaOH and ball milling for 10 min. The liquid fraction after treatment contained by-products such as monosaccharides, oligosaccharides, acetic acid, and lignin but no furfural and 5-hydroxymethylfurfural. Quantitative correlation analysis showed that YG = 41.41 + 4.09´ball milling time (min) + 112.7´NaOH concentration (%) (R2 = 0.87), YG = 37.79´cellulose content - 1421.55 (R2 = 0.88), and YG = -72.47´lignin content + 1482.77 (R2 = 0.95).Conclusion: The impact of the physical and chemical properties of corn stover pretreated with different ball milling times and/or different NaOH concentrations on the subsequent enzymatic hydrolysis was investigated, which would be beneficial to illustrate the effective mechanism of mechanochemical pretreatment method.
In Vitro Screening of Antioxidant, Antimicrobial and Anticancer Activities of Cyanobacteria Found Across Odisha Coast, India
Authors: DASH SATYABRATA , PARIDA SABYASACHY , SAHOO BIJAYANANDA , RATH BISWAJIT 
Published: January 2022

Abstract
Cyanobacteria contain a rich source of several bioprospective molecules with antibacterial, antifungal, antioxidant, and anticancer properties. The objective of the current study was determine the antioxidant, antimicrobial and anticancer potential of methanol and acetone extracts of two cyanobacterial species (Lyngbya majuscule and Lyngbya martensiana) isolated from Odisha coast. The free radical scavenging assay of the extracts was performed against DPPH and ABTS+. The reducing power assay of the extracts was also determined. The maximum DPPH and ABTS radical scavenging activity is recorded on L. majuscule and the IC50 values were 251.34±0.96 and 282.24±0.87 µg/ml respectively. Similarly, the reducing power assay was also highest in L. majuscule (0.762±0.0015). The total phenolic (69.18±0.79 µg gallic acid equivalents g−1) and total flavonoid (38.21±0.61 µg quersentin equivalents g−1) was found more in methanolic extract. The methanol extract of L. majuscule exhibited higher antimicrobial activity (23±0.74 mM) as compared to other test species. Further, methanol extract of L. majuscule, active fractions was analysed by gas chromatography-mass spectrometry (GC-MS), and bioactive compounds like 1-Monolinoleoylglycerol trimethylsilyl ether (31.182%) and n-Hexadecanoic acid(15.42%)were detected. Similarly the cytotoxicity results exhibited decreasing cell viability of HepG2 cells in methanol extracts L. majuscule in a dose-dependent manner without cytotoxicity on normal cells.These results concluded that extracts of studied cyanobacterial species exhibited appreciable antioxidant and antimicrobial activity and further the methanol extract of L. majuscule possess sufficient amount of bioactive compounds having antioxidant, antimicrobial and anticancer activity and thus could be a promising source for health products.
Experimental and Theoretical Approaches for Polyvinylchloride Enhanced with Metal Oxides/Graphene
Authors: Ezzat Hend A. , Hegazy Maroof A. , Yahia Ibrahim S. , Zahran Heba Y. , Elhaes Hanan , IBRAHIM Medhat 
Published: January 2022

Abstract
Introducing graphene (G) into the polymer matrix is supposed to improve both the electrical and optical characteristics, especially in space applications. To study the effect of metal oxide and G on PVC, a matrix of PVC/ZnO/G nanocomposite was prepared and studied using different spectroscopic techniques. As a consequence, the Fourier Transform Infrared (FTIR) spectra for PVC/ZnO/G revealed two significant bands at 1596 cm-1 and 515 cm-1, G and ZnO, respectively, confirming the composite synthesis. Also, X-ray Diffraction (XRD) and Field-emission Scanning Electron Microscopy (FESEM) result confirmed the interaction between PVC, ZnO, and G. Density functional theory (DFT) calculations were performed at B3LYPL/LAN2DZ on PVC nanocomposites with various metal oxides such as MgO, SiO2, TiO2, NiO, CuO, ZnO, and ZrO2 and then reinforced with graphene quantum dots (GQDs). The findings of the HOMO/LUMO band gap energy, total dipole moment (TDM), and Molecular Electrostatic Potential (MESP) indicated that the effect of MgO in the presence of GQD gives a nanocomposite with high electronic characteristics with a TDM of 16.675 Debye ∆E 0.231 eV. The distinctive properties of such nanocomposite PVC/MgO/GQD ZTRI C46 enabling usage in optical and electrical components and systems, as well as anti-reflection coating applications for solar cells.