bluuebunny/crossref_metadata_2025_split huggingface dataset:
District Health Office Efforts and Challenges In Combating P. Knowlesi Malaria: A Qualitative Study Of High Risk States In Peninsular Malaysia
Authors: Mohamad Emma , Azlan Arina Anis , Hamzah Mohammad Rezal , Damanhuri Hazwani , Pasi Hafizah , Sulong Mohd Rahim , Isa Affendi 
Published: April 2022

Abstract
Background: P. knowlesi malaria remains a severe threat for at-risk communities in Malaysia. This study explores efforts, challenges and initiatives taken by the health officers at districts with high cases of P. knowlesi malaria in peninsular Malaysia in their role as health authority for mitigating the disease.Method: In-depth interviews were conducted with three health officers from Gua Musang, Kuala Lipis and Kota Tinggi, where high cases of P. knowlesi malaria were recorded. A purposive sampling strategy was utilised to select each district's health officers involved with malaria prevention efforts. The interviews were transcribed and analysed using Atlas.ti 8 to identify current malaria prevention practices and the challenges faced in the implementation of these practices.Results: Efforts by the health district officers include conventional prevention strategies and inventive measures to mitigate infection. The conventional strategies were erecting warning signs, community education programmes and empowering community influencers. Health district officers also initiated unconventional mitigation strategies, including engagement with indigenous communities and foreign workers, erecting makeshift dams and using Abate 500E formulation sachet in rivers. Challenges faced include difficulty accessing remote locations, limited facilities, bureaucratic matters and military-specific issues.Conclusion: District health officers have made many efforts to mitigate P. knowlesi infections, including unconventional initiatives. Despite the challenges faced, the district health officers displayed commitment, creativity, and determination to combat P. knowlesi malaria.
Citizen engagement in health care procurement decision-making by health care insurers: Recent experiences in the Netherlands
Authors: Fernandes Óscar Brito , Bos Véronique , Klazinga Niek , Kringos Dionne 
Published: April 2022

Abstract
Background
In insurance-based health care systems, health care insurers are interested in engaging citizens in care procurement to contract health care services that matter to people. In the Netherlands, an amendment to the Health Insurance Act was set forth in 2021 to formalise and strengthen the engagement of the insured population with health care insurers’ procurement cycles. This study explores the role of Dutch health care insurers in operationalising citizen engagement in procurement cycles before changes occur linked to the amendment to the Health Insurance Act.
Methods
An exploratory qualitative study was conducted in two phases: 1) we consulted academics and policy experts on the role of health care insurers regarding citizen engagement; 2) we conducted focus groups with representatives of health care insurers to understand how citizens’ engagement is being operationalised. Transcripts of the interviews with experts and detailed notes of focus group meetings were analysed using a qualitative inductive approach. Selected excerpts were analysed on discourse and content and organised by a coding scheme following a rigorous and accelerated data reduction technique.
Results
We identified four strategies used by health care insurers to operationalise citizen engagement: 1) broadening their population health orientation; 2) developing and improving mechanisms for engaging citizens; 3) strengthening features of data governance for effective use of value-driven data; 4) implementing financial and incentive mechanisms among health care providers in support of value-based health care. However, regulated market mechanisms and low institutional trust in health care insurers undermine their transitioning from merely funding health care towards becoming people-centred value-based health care purchasers.
Conclusion
Dutch health care insurers seem to be strengthening the community orientation of their functioning amid enhancing the end-to-end experience of the insured. The expected practical effects of the amendment to the Health Insurance Act are broadening the role of the council of insurees in decision-making processes and systematically documenting the efforts set forth by health care insurers in engaging citizens. Further research is needed to understand better how the regulated competitive market could be hampering the engagement of citizens in health care procurement decision-making and value creation from the citizens’ perspective.
Ultra-large-scale, fast, one-step and facile Synthesis of Iron, Nickel, Cobalt, Manganese and Zinc Oxide Nanoparticles by a new and one pot Solution Combustion synthesis method
Authors: Yeganeh-Faal Ali , Habibi-Khaveh Alireza. 
Published: April 2022

Abstract
Here, we report one-step, new, low cost, fast and innovative synthesis by solution combustion synthesis (SCS) method, in order to ultra-large-scale synthesis magnetic nanoparticles of Fe3O4, NiO, Co2O3, ZnO and Mn3O4. The synthesis, was performed in the temperature 350 ° C in a nitrogen atmosphere and in the presence of poly maleic acid as dispersant and fuel agent. Ammonium nitrate, was used as a gasser agent in acidic medium of HNO3. FTIR and UV-Vis spectroscopy were used for study spectroscopic properties of SCS product nanomaterial. Field emission scanning electron microscopy (FESEM), Energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD) were used to study the microstructure and phase of nanomaterial. VSM for magnetic properties and modified Scherer method, used to determine particle size. This study provides a safe, one-step, facile, large-scale and general method for the synthesis of metal oxide nano materials. Due to the possibility of forming a single layer of synthesized nanoparticles, the biggest advantage of this method compared to other combustion synthesis methods is to obtain nanoparticles with relatively the same particle size. This is a unique result, which is not seen in the combustion methods of nanoparticle synthesis.
Primary Research on the Indigenous Copper Production of Bohai Kingdom
Authors: Li Chenyuan , Wu Song , Li Qiang , Song Yubin , Ying Jia , Li Yanxiang 
Published: April 2022

Abstract
The systematic investment and rearrangement of the unearthed artifacts of Longtoushan Royal Cemetery of the Bohai Kingdom have provided a bunch of new information for the archaeometallurgy research on non-ferrous metal production. We have synthesized the latest discoveries of several relative mining/smelting sites and urban relics for making a primary approach to the whole production chain of copper in this research. The distinction between the red copper and bronze artifacts was unveiled by analyzing the data from SEM-EDS and p-XRF. The yield gap between the scale of smelting and the number of artifacts was also discussed based on the historical literature records. The evidence and detail of indigenous copper production could help the researcher comprehend the technology and material exchange and geopolitical relationship development of the Bohai Kingdom.
Steel Slag as Low-Cost Catalyst for Artificial Photosynthesis: From CO2 to Hydrogen and Methanol
Authors: Fusco Caterina , Casiello Michele , Pisani Pasquale , Monopoli Antonio , Oberhauser Werner , Attrotto Rosella , Nacci Angelo , D'Accolti Lucia 
Published: April 2022

Abstract
Photoreduction of CO2 with sunlight to produce solar fuels, also named artificial photosynthesis, is considered one of the most attractive strategies to face the challenge of reducing greenhouse gases and achieving climate neutrality. Following an approach in line with the principles of the circular economy, the low-cost catalytic system (1) based on an industrial by product such as steel slag was assessed, which was properly modified with nanostructured palladium on its surface in order to make it capable of promoting the conversion of CO2 into methanol and hydrogen through a two-stage process of photoreduction and thermal conversion having formic acid as the intermediate. Notably, for the first time in the literature steel slag is used as photoreduction catalyst.
A Novel Necroptosis-Related lncRNA Signature Predicts the Prognosis of Bladder Cancer
Authors: Li Han , Lv Zhengtong , Liu Ming 
Published: April 2022

Abstract
Background: Necroptosis is a programmed necrotic cell death pathway that closely related to tumor progression. Long noncoding RNAs (lncRNAs) have multiple functions in the cancer immunity response. In the study, we aim to construct a necroptosis -related prognostic lncRNA signature in bladder cancer (BC) and explore its precise treatment implications in BC.Methods: We downloaded clinicopathology and transcriptome information from The Cancer Genome Atlas (TCGA) and form the synthetic data matrices and performed coexpression analysis and univariate Cox regression to get the Necroptosis-related prognostic lncRNA. The prediction model was constructed using Lasso regression. The Kaplan-Meier method was used to analyze the overall survival (OS) rate of the high-risk and low-risk groups. Time-dependent receiver operating characteristics (ROC), univariate Cox (uni-Cox) regression, multivariate Cox (multi-Cox) regression were performed to evaluate the model. Gene set enrichment analyses (GSEA), principal component analysis (PCA), immune analysis, and prediction of the half-maximal inhibitory concentration (IC50) in risk groups were also analyzed. Consensus clustering analysis based on necroptosis-related lncRNAs was made to get two clusters to identify hot tumors and cold tumors. Finally, the immunotherapy between the cold and hot tumors was discussed.Results: We constructed a signature composed of 5 necroptosis-related lncRNAs (LINC01140, Z98200.1, AC010168.2, Z84484.1, AC004076.2). Compared with the low-risk group, the high-risk group had a worse prognosis. Compared with clinicopathological variables, the necroptosis -related lncRNA signature demonstrated good predictive ability on the prognosis of patients with BC. The area under the receiver operating characteristic curve of risk score for predicting 5 year OS were 0.690. Immune function and immune checkpoint expression differ significantly between the low-risk and high-risk groups. IC50 between risk groups show that high risk patients were more sensitive to conventional chemotherapy drugs. The cluster 1 was confirmed as hot tumors that had better immune infiltration and immune scores, which is more susceptible to immunotherapeutic and chemotherapy drugs.Conclusion: The necroptosis-related lncRNA signature constructed is convenient to predict prognosis and classify hot or cold tumors of BC patients, so as to achieve precision therapy for BC.
Effects of supplementation of pomegranate processing by-products and waste cooking oils as alternative feed resources in broiler nutrition
Authors: Ghasemi-Sadabadi Mohammad , Ebrahimnezhad Yahya , Maheri-Sis Naser , Shaddel-Teli Abdolahad , Ghalehkandi Jamshid Ghiasi , Veldkamp Teun 
Published: April 2022

Abstract
Agricultural residues can be used as alternative feed sources in industrial chicken production. The impacts of different levels of pomegranate peel and waste cooking oil as an agricultural residue on broilers nutrition were investigated. Results showed that replacement of 8% pomegranate peel in diets decreased growth performance of broilers. Supplementing 8% pomegranate peel in diets reduced apparent nutrient digestibility. The highest level of waste oil inclusion in broiler diets indicated negative impacts on apparent metabolisable energy and crude fat apparent nutrients digestibility. Broilers fed the diet containing 4% pomegranate peel had higher Lactobacillus population. The results showed that Lactobacillus population was lower in broilers fed 8% pomegranate peel powder and 4% waste oil in diets. Inclusion of 8% pomegranate peel powder in diets was showed lower villus height and crypt depth in duodenum, jejunum and ileum. Inclusion of 4% pomegranate peel decreased peroxide value (PV) of meat. Dietary inclusion of 4% waste oil raised PV of meat. Alpha-tocopherol supplementation decreased PV of meat. Finally, the results provide information that 4% of pomegranate peel and 4% waste oil could be used as an alternative feed ingredient in broiler diets without adverse effects.
Chill coma recovery of Ceratitis capitata adults across the Northern Hemisphere
Authors: Papadopoulos Nikos T. , Moraiti Cleopatra A. , Verykouki Eleni 
Published: April 2022

Abstract
The Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae), is an invasive pest, that is currently expanding its geographic distribution from the Mediterranean coasts to more temperate areas of Europe. Given that low temperature is a primary determinant of insect species’ range boundaries especially in the Northern Hemisphere with pronounced seasonality, we used chill coma recovery time for assessing latitudinal clines in basal chill tolerance of C. capitata adults. We selected six populations obtained from areas with broad climatic variability based on the main bioclimatic variables of temperature and precipitation, spanning a latitudinal range of about 19oN from Middle East to Central Europe. Adults were exposed to 0oC for 4h, and time to regain the typical standing position of a fly at 25oC were recorded. The post-stress survival after a period of 8 days was also recorded. Results revealed that adults from Israel and Austria were less chill tolerant than those from Greece, resulting in curvilinear trends with latitude. Analysis of macroclimatic conditions revealed combined effects of latitude (as a proxy of photoperiod) and macroclimatic conditions on chill coma recovery time. Nonetheless, there was not a deleterious effect on post-recovery survival, except for flies obtained from the northern most point (Vienna, Austria). Overall, it seems that evolutionary patterns of basal chill coma recovery time of C. capitata adults are driven mainly by local climatic variability.
Synthesis of composites for the removal of F^- anions
Authors: Damian-Ascencio C. E. , Arcibar J. A. , Guerrero L. , Saldana-Robles A. , Marquez A. , Corona M. , Gallegos-Muñoz A. , Cano-Andrade S. 
Published: April 2022

Abstract
This work presents the synthesis of amine and ferrihydrite func-tionalized graphene oxide for the removal of fluoride from water. The synthesis of the graphene oxide and the modified with amine groups is developed by following the modified Hummer’s method. Fourier transform infrared spectrometry, X-ray, Raman spectroscopy, 1 Springer Nature 2021 L A T E X template S. of Composites for the removal of F − thermogravimetric analysis, surface charge distribution, specific surface area and porosity, adsorption isotherms, and the van’t Hoff equation are used for the characterization of the synthesized materials. Results show that the addition of amines with ferrihydrite generates wrinkles on the surface layers, suggesting a successful incorporation of nitrogen onto the graphene oxide; and as a consequence, the adsorption capacity per unit area of the materials is increased.
Lifelong nnUNet: a framework for standardized medical continual learning
Authors: Gonzalez Camila , Ranem Amin , Santos Daniel Pinto dos , Othman Ahmed , Mukhopadhyay Anirban 
Published: April 2022

Abstract
As the acceptability of Deep Learning solutions grows, both medical practitioners and regulatory bodies are exploring ways to safely introduce image segmentation solutions in clinical practice. One frontier to overcome when translating promising research into the clinical open-world is the shift from static to lifelong learning. Continual learning, the practice of training models sequentially with shifting population dynamics, is seeing growing interest but is still at its infancy in healthcare. We present Lifelong nnUNet, a standardized framework for medical image segmentation that places state-of-the art continual segmentation at the hands of researchers and clinicians. Build on top of the popular nnUNet and equipped with all necessary modules for training and testing continual models, we ensure broad applicability and lower the barrier for evaluating new solutions in a sequential fashion. We introduce benchmark results on three medical segmentation use cases with open-source datasets, and provide the tools to quickly test five continual learning methods on further applications.
GSNOR modulates oxygen and glucose deprivation-induced brain microvascular endothelial cells injury via HIF-1α S-nitrosylation associated mitophagy activation
Authors: Han Zhenping , Wang Siyi , Li Tingting , Gao Li , Zhao Yu , Li Han-bing , Huang Ping , Yan Jie-ping 
Published: April 2022

Abstract
Denitrosylase S-nitrosoglutathione reductase (GSNOR) regulates the equilibrium of total cellular S-nitrosylation levels, a key redox-based post-translational modification that is frequently dysregulated in various diseases. However, the effects and underlying mechanisms of GSNOR on brain microvascular endothelial cells (BMECs) under hypoxia conditions are unknown. In this study, a photothrombosis model was used to induced cerebral ischemia injury in the male C57BL6 mice injury. BMECs were subjected with oxygen-glucose deprivation (OGD) for 6 h to establish the ischemic cell models in vitro. Increased GSNOR levels and S-nitrosylation dysfunctions were observed in cerebral ischemic injury. GSNOR knockdown rescued OGD-induced ischemic injury in BMECs. HIF-1α S-nitrosylation was significantly increased in BMECs after GSNOR knockdown. We investigated the effects of GSNOR on mitochondrial functions and revealed the relationship between HIF-1α translocation and downstream signaling, including mitochondrial bioenergetic functions and activation of mitophagy in BMECs. GSNOR knockdown alleviated OGD-induced mitochondrial impairments. Moreover, GSNOR reduced excessive mitochondrial reactive oxygen species (ROS) production, thereby, alleviating mitochondrial oxidative stress. GSNOR knockdown also enhanced the disturbance of cellular bioenergetic functions and BNIP3L/NIX-mediated mitophagy. Expression levels of mitophagy-related proteins, such as LC3B, BNIP3L/NIX, and FUNDC1were significantly elevated in GSNOR knockdown detected by western blot. These results suggest that the knockdown of GSNOR knockdown alleviates mitochondrial toxicity by promoting HIF-1α S-nitrosylation-associated repair of mitochondrial functions and activating mitophagy in BMECs. Understanding the protective effects of GSNOR provides a novel strategy for developing potential therapies targets for ischemic injury in BMECs.
Global stability and bifurcation of macroscopic traffic flow models for upslope and downslope
Authors: Cen Bing-Ling , Xue Yu , Qiao Yan-Feng , Wang Yi , Pan Wei , He Hong-Di 
Published: April 2022

Abstract
A macro continuum model of the traffic flow is derived from a micro car-following model that considers both the upslope and downslope by using the transformation relationship between macro- and micro variables. The perturbation propagation characteristics and stability conditions of the macroscopic continuum equation are discussed. For uniform flow in the initial equilibrium state, the stability conditions reveal that as the slope angle increased under the action of a small disturbance, the upslope stability increases and downslope stability decreases. Moreover, under a large disturbance, the global stability analysis is carried out by using the wavefront expansion technique for uniform flow in the initial equilibrium state. For the initial nonuniform flow, nonlinear bifurcation analysis is conducted at the equilibrium point. Subcritical Hopf bifurcation exists when the traffic flow state changes,thus the limit cycle formed by the Hopf bifurcation is unstable. Simulation results verify the stability conditions of the model and determine the critical density range. The numerical simulation results show the existence of critical Hopf bifurcation in phase space, and the spiral saddle point varies with the slope angle. Furthermore, the impact of the angle of both the upslope and downslope on the evolution of density waves is investigated.
Variations in uniform hazard spectra and disaggregated scenarios during earthquake sequences
Authors: Azarbakht Alireza , Douglas John 
Published: April 2022

Abstract
Seismic hazard varies greatly during an earthquake sequence. Understanding this variation can be useful to end-users, such as emergency managers, as it would enable them to make more informed decisions about potential risk reduction measures. This article presents examples of how two commonly-used products of probabilistic seismic hazard assessments: uniform hazard spectra and disaggregated earthquake scenarios, vary during two seismic sequences in western Greece. These calculations are made using a recent time-dependent seismic hazard model based on a Bayesian ETAS approach. The examples show that time-dependent uniform hazard spectra for short return periods (1 and 10 years) are significantly higher than standard time-independent spectra but that uniform hazard spectra for the commonly-used return periods of 475 and 2475 years are similar to those from time-independent assessments. The time-dependent spectra generally converge within a couple of days to the time-independent spectra. The examples also show that the dominant earthquake scenarios evidenced by the disaggregation for the time-dependent assessment can show significant differences from the time-independent scenarios. This is particularly true when the earthquake sequence is distant from the location of interest as the aftershocks contribute greatly to the overall hazard. To show these changes more clearly this article introduces a new graphical representation of the disaggregated results.
Two novel qPCR assays to enhance black rail (Laterallus jamaicensis) eDNA surveys in the United States
Authors: Feist Sheena M. , Guan Xin , Malmfeldt Madeleine P. , Lance Richard F. 
Published: April 2022

Abstract
Black rails (Laterallus jamaicensis) are a rare and protected bird species. They are also notoriously difficult to monitor. eDNA surveys have proven beneficial for the detection of this elusive species, but additional developments are needed to enhance eDNA survey utility (including reliability of outcomes) in light of black rail status and associated high-profile conservation actions. The use of multiple assays functions to increase eDNA detection confidence by increasing the probability of amplification in low concentration, highly degraded samples (i.e., reducing the risk of false negatives) and by providing a multiplicative test for ruling out contamination as the source of qPCR amplification (i.e., reducing the risk of false positives). The two eDNA assays developed, optimized, and validated in this study will prove valuable for the continued surveillance of the secretive black rail, offering data to measure its distribution and occurrence over time and as influenced by anticipated climate-change-induced disturbances and/or habitat restoration/creation initiatives. The assays are applicable to both Eastern black rails and California black rails, and do not exhibit qPCR amplification in non-target, co-distributed taxa (including other Rallidae species) in the United States.