bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the Inhibitory Effects of Blebbistatin on Actomyosin Interactions in Myofibrils and Isolated Myofilaments
Authors: Elkrief Daren , Cheng Yu-Shu , Rassier Dilson E. 
Published: February 2025

AbstractMyosin II is the molecular motor responsible for muscle contraction. Myosin II hydrolyses ATP into Piand ADP, to convert chemical energy into mechanical work while attached to actin filaments. The relation between force generation and Pirelease remains unclear. Many studies use chemical substances, such as blebbistatin, to study the transitions during actomyosin interactions. Blebbistatin and its derivatives selectively inhibit the actin-activated ATPase of myosin II, accumulating myosin cross-bridges in a pre-power-stroke state. Although the effects of blebbistatin have been explored, it is still unclear how blebbistatin affects force generation and the velocity of contraction. In this study, we used individual myofibrils, myosin and actin filaments, and isolated heavy meromyosin (HMM) and actin filaments to characterize the effects of blebbistatin. We observed that increasing concentrations of blebbistatin (i) decreased the force produced by myofibrils and isolated myosin filaments, (ii) decreased the maximum velocity of shortening produced by myofibrils and the myosin-induced actin sliding velocity, (iii) decreased the curvature of the force-velocity relation in a dose-dependent manner. Furthermore, UV radiation reduced the effect of blebbistatin, which was partially reversed if blebbistatin was bound to myosin before exposure to UV light. These results show that blebbistatin alters force and velocity generation at the molecular, myofilamentous and myofibrillar levels. This study has interesting implications in fields which rely on using blebbistatin to study cellular processes and confirms several results published in different experimental arrangements. Thus, this study is exploratory and confirmatory, and the findings have utility surrounding cell migration, muscle biophysics, cellular reproduction, or any processes that rely on the action of myosin II.
LAVR-289, a new acyclo-nucleoside phosphonate having broad-spectrum activity against herpesviruses
Authors: Kappler-Gratias Sandrine , Quentin-Froignant Charlotte , Marcheteau Elie , Fernandez José , Boutolleau David , Garcia Virginie , Roy Vincent , Agrofoglio Luigi A. , Gallardo Franck 
Published: February 2025

ABSTRACTHuman herpesviruses are latent opportunistic large dsDNA viruses that can have deleterious effect in immunocompromised patients by triggering life-threatening infections. Over the past years, different antivirals have been developed against a variety of herpesviruses, including Acyclovir for herpes simplex viruses (HSV1 and 2) and ganciclovir and letermovir for human cytomegalovirus (hCMV). However, broad-spectrum inhibitors of herpesvirus infections are still missing. Here we report the efficacy of LAVR-289, a new acyclic nucleoside analog, on a broad variety of herpesviruses from human and animal origin. LAVR-289 displays nanomolar efficiencyin vitro, is active on viral strains resistant to gold standard antivirals andex vivoon reconstituted human skin infected with HSV1. Combined with its anti-poxvirus and anti-adenovirus activity, LAVR-289 can become the next gold standard for the management of opportunistic virus infections in immunocompromised patient.
The critical role of enterovirus 2A protease in viral translation, replication, and antagonism of host antiviral responses
Authors: Schipper Jelle G. , Aloise Chiara , Sutter Sereina O. , Zwaagstra Marleen , van Vliet Arno L.W. , Abdelnabi Rana , Ignacio Bob , Bonger Kimberly M. , Roelofs Dagmar , van den Brand Judith M.A. , Wubbolts Richard W. , Bruurs Lucas J.M. , Thibaut Hendrik Jan , Neyts Johan , Tanenbaum Marvin E. , van Kuppeveld Frank J.M. 
Published: February 2025

AbstractEnteroviruses dramatically remodel the cellular infrastructure for efficient replication and curtailing host antiviral responses. Proteases 2Aproand 3Cprohave been implicated in these processes based onin vitrostudies, ectopic overexpression, and surrogate infection systems, but their relative contributions are unknown. Here, we replace the essential 2A cleavage site at the P1-P2 junction with an internal ribosome entry site (IRES), 3CD cleavage site, or T2A sequence, allowing us to catalytically inactivate 2Apro. Viruses with an inactive 2Aproare hampered in replication in cell lines and are severely attenuated in a Coxsackievirus B3 (CVB3) mouse pancreatitis infection model. We show that 2Apro, but not 3Cpro, is essential during infection for disturbing nucleocytoplasmic transport, shutting down host mRNA translation, suppressing stress granule formation, cleaving retinoic acid-inducible gene-1 (RIG-I)-like receptor (RLR) signaling pathway proteins and suppressing interferon-α/β (IFN-α/β) transcription, and overcoming the antiviral action of IFN-induced restriction factors. Moreover, using an advanced single-molecule live cell imaging approach, we reveal that 2Aprois important for the initial round of replication of the incoming viral RNA, which is a bottleneck for efficient infection. In conclusion, we establish that 2Aproplays a critical role in subverting antiviral responses and establishing a favorable host environment to expedite enterovirus replication.
Determining project control system effectiveness in construction project delivery
Authors: Alotaibi Rashed , Sohail M. , Edum-Fotwe F.T. , Soetanto Robby 
Published: February 2025

PurposeMany construction projects exhibit poor performance in terms of fulfilling predetermined schedules and financial objectives. Project control systems (PCSs) have been used to enhance construction project performance; however, a comprehensive framework regarding the key determinants of PCS effectiveness is lacking.Design/methodology/approachHerein, the determinants for effective PCSs that can improve construction project performance were comprehensively identified by evaluating existing studies. A systematic strategy following the preferred reporting items for systematic reviews and meta-analyses protocol was employed to search for and select relevant studies, followed by a qualitative synthesis.FindingsThe significance of incorporating and managing many factors associated with PCS for effective project delivery was elucidated. The study synthesized 12 key determinants and 29 sub-determinants of PCS effectiveness in project delivery and grouped them into organizational, human, technological and operational categories. Out of the four categories examined, operational aspects received the most references, underscoring their critical role in PCS effectiveness, while human-related dimensions received the least amount of attention in the reviewed research, accounting for 4%. This also revealed a significant gap in the research addressing the interactions between all PCS aspects.Originality/valueUnderstanding of the variables influencing PCS effectiveness in construction project delivery was enhanced, and a framework for future PCS research in five dominant areas was created.
Enhancing ESG integration in corporate strategy: a bibliometric study and content analysis
Authors: Almnadheh Yazan , Samara Husni , AlQudah Mohammad Zakaria 
Published: February 2025

Purpose
This study aims to develop a comprehensive framework for environmental, social and governance (ESG) integration in corporate strategy by differentiating between four mechanisms: legality, ethical concerns, monitoring and motivation. These mechanisms are examined from governance and performance perspectives to provide a deeper understanding of their implications.

Design/methodology/approach
A systematic review of the Web of Science database formed the basis of this study. Descriptive analyses tracked publication trends, while detailed content analysis identified key themes. These findings were synthesized into a conceptual framework for ESG integration, ensuring practical relevance for scholars and practitioners.

Findings
The study identifies areas where ESG integration influences corporate strategy, such as enhanced corporate governance, ethical decision-making, risk management and improved organizational performance. Notable trends include the increasing emphasis on regulatory compliance and ethical concerns in corporate governance, reflecting shifts in priorities across academic and industry domains. The framework provides a foundation for advancing research and strategic ESG implementation.

Practical implications
The findings offer actionable insights for corporate strategists and policymakers to enhance risk management, operational efficiency and corporate reputation through ESG adoption.

Social implications
Enhanced ESG integration fosters social equity and sustainable development by promoting ethical practices and addressing stakeholder concern.

Originality/value
This research introduces a novel framework that categorizes drivers and consequences of ESG adoption, bridging gaps in the literature.
Innovators and Transformers: Enhancing supply chain sustainability: a methodology combining dynamic simulation and case studies
Authors: Negri Pietro 
Published: February 2025

PurposeThis paper scrutinizes existing assessment methodologies in supply chain sustainability literature and identifies a gap in microeconomic methods for comprehensively estimating current and prospective supply chain performances, encompassing both financial and environmental aspects. It presents a case study where quantitative, dynamic simulation modeling is applied to articulate and amplify a company’s sustainability performance, demonstrating that a holistic approach to performance metrics can significantly refine decision-making and action prioritization in sustainability.Design/methodology/approachIntroduced here is the “ISSSC Approach, ” which employs dynamic simulation modeling to quantify the flux of materials, value, and emissions within a supply chain. This methodology facilitates the construction of representative models and subsequent scenarios to inform sustainable decision-making that aligns with business objectives. The utility of the ISSSC Approach is evidenced through a case study within the dairy industry.FindingsThe case study offers three pivotal insights: the innovation of a fact-based sustainability framework over traditional anecdotal methods, the harmonization of competitive and sustainable performance indicators within a company’s strategic ambit, and the presentation of a novel tool for precise sustainability measurement and enhancement, attuned to context-specific constraints.Originality/valueWhile dynamic simulation modeling is well-established, its application to sustainability in a manufacturing context is markedly innovative. The ISSSC Approach bridges the gap between regulatory directives and the realization of tangible benefits for all stakeholders, positioning it as a critical tool for operationalizing sustainability in corporate strategy.
Understanding user behaviors toward food delivery app services: the moderating effects of generation and usage frequency
Authors: Alam Mirza Mohammad Didarul , Hussain Khalid , Nusair Khaldoon , Momotaz Shamsun Nahar 
Published: February 2025

PurposeThis paper investigates the influence of e-service quality (process and user experience) and price fairness on customer satisfaction, which, as a consequence, affects brand love toward Food Delivery App (FDA) services. More importantly, it examines the moderating roles of generation (Gen Y vs Gen Z) and usage frequency (low vs high) in affecting user behaviors.Design/methodology/approachData were collected from 354 food delivery app users (Gen Y and Z) in Bangladesh through structured questionnaire and analyzed with PLS-SEM.FindingsThe findings show that, apart from reliability, all the dimensions of service quality influence app users’ satisfaction, which, in turn, enhances their brand love. Furthermore, the outcome reveals that the impact of customer satisfaction on brand love is stronger among Gen Y compared to Gen Z. Finally, the outcomes suggest that as users use the FDA services more frequently, the relationship between customer satisfaction and brand love weakens.Practical implicationsThe study outcomes facilitate the FDA service providers and restaurant operators to focus on the quality and price of services to ensure the satisfaction and brand love of Gen Y and Gen Z. Separate policies must be taken for Gen Y and Gen Z to manage their brand love towards FDA services.Originality/valueGrounded on the S-O-R paradigm and the Generational Cohort theory, this study expands the knowledge horizon of FDA services by focusing the behavior of Gen Y and Gen Z. More specifically, investigating how the impact of user satisfaction on brand love towards FDA services changes across generations and usage frequency are the novel contributions of this study.
Open strategizing by public organizations: a qualitative comparative analysis of recent practices in Dutch infrastructure planning
Authors: Baartmans Manon Danielle , Busscher Tim , Verweij Stefan , Arts Jos 
Published: February 2025

PurposeWide participation in the strategic processes of public organizations can enhance inclusion and transparency, thereby contributing to improved performance of public organizations. However, engaging with external participants is easier said than done. This study aims to identify how public organizations can open up their strategic processes to external participants.Design/methodology/approachThe analysis is based on qualitative data from 12 early-phase strategy projects in the 2023 Dutch Multi-Year Program for Infrastructure, Spatial Planning and Transport (MIRT). Interviews and document data were collected and analyzed using qualitative comparative analysis (QCA).FindingsThis study identifies that the government steering the process itself, by aiming for idea generation, opens up strategic processes. This is necessary for contributing to improved public service performance.Practical implicationsThis study provides strategy actors (e.g. managers, planners and consultants) with actionable knowledge about what open strategizing with external participants requires to create openness.Originality/valueThis study addresses the ongoing and increased interest in the openness of strategy processes in general and in infrastructure planning in particular. It contributes to the discussion on whether and how participation leads to improved performance of public organizations. Additionally, this study illustrates the application of QCA to the study of open strategy processes.
Technological acceptance in the retail sector: an empirical generalisation by a meta-analytic study
Authors: Santini Fernando de Oliveira , Maçada Antonio Carlos Gastaud , Freitas Junior José Carlos , Ladeira Wagner Junior , Oliveira Tiago 
Published: February 2025

Purpose
The purpose of this study is to present a systematic analysis using a meta-analytical approach regarding the antecedents and consequences of the technology acceptance model (TAM) in the retail context. This paper also analysed five different theoretical models using meta-analytic structural equation modelling. Finally, this paper investigated possible moderators on the relationships between TAM constructs.

Design/methodology/approach
This manuscript is conceptual as it is based on a literature review. In this line, this paper analysed 85 articles by a meta-analysis that generated 431 effect sizes and identified 8 antecedents and 2 consequences of the TAM construct.

Findings
All antecedents and consequences promoted significant effect sizes with TAM constructs. In the moderation analysis, this paper determined that top journals and countries with a high human development index promoted more substantial effect sizes in the relationship between perceived usefulness (PU) and behavioural intention, and PU and attitude towards system use, respectively. About the theoretical models tested, this paper found that the antecedents enjoyment and trust represent the best fit in TAM models, which affects attitude and behavioural intentions.

Originality/value
This research tested the impact of TAM’s antecedents, consequences and moderators in the retail sector and presented important results via a meta-analytical review. This meta-analysis contributes to the marketing literature by offering a set of empirical generalisations, including relationship coefficients and calculated fail-safe numbers.
The Dunning–Kruger effect and artificial intelligence: knowledge, self-efficacy and acceptance
Authors: Guan Jian , He Xiao , Su Yuhan , Zhang Xin-an 
Published: February 2025

PurposeArtificial Intelligence (AI) is revolutionizing the world. Despite the numerous advantages of AI in terms of faster processing and higher efficiency, AI hasn’t been widely accepted by humans yet. This study aims to shed light on this phenomenon by exploring the Dunning–Kruger Effect in AI knowledge and examining how AI knowledge affects AI acceptance through AI-related self-efficacy.Design/methodology/approachBy collecting data from 179 managers, we examined the Dunning–Kruger Effect in AI knowledge and used mediation analysis to explore the mechanisms by which AI knowledge leads to AI acceptance.FindingsOur findings indicated the presence of the Dunning–Kruger Effect in AI knowledge. Furthermore, our results revealed that AI knowledge has a nonlinear effect on AI acceptance through AI-related self-efficacy.Originality/valueIn contrast to previous research that posited a linear link between knowledge and acceptance of technology, this study offers a new framework for the nonlinear relationships between AI knowledge, AI-related self-efficacy and AI acceptance by extending the Dunning–Kruger effect to the AI field.
Does humanizing the police improve the effectiveness of police recruiting efforts?
Authors: Hill Shawn L. , Brimbal Laure , Maguire Edward R. , Stephens Eve 
Published: February 2025

AbstractResearch SummaryU.S. police agencies are currently facing a serious recruiting and staffing crisis. As a result, many scholars and police leaders are re‐examining police recruiting methods. This study draws on the field of intergroup communication to examine the effects of how police are portrayed in police recruitment materials. Using a randomized survey experiment, we tested the effects of humanizing how officers are portrayed in a recruiting video relative to a more traditional, action‐oriented portrayal. We showed police recruiting videos to a sample of 325 university students. Within the videos, we varied how a female police officer described her career and her personal life. We then measured the extent to which the treatment influenced participants’ general attitudes toward police and specific attitudes toward working in policing. The treatment had significant effects on the two general attitudinal outcomes (shared identity and institutional trust), but did not have a significant direct effect on respondents’ interest in working in policing. Notably, the treatment had significant indirect effects on respondents’ interest in a career in policing via shared identity, but not via institutional trust.Policy ImplicationsApplying principles from the study of intergroup communication can help police agencies develop recruiting materials that humanize the police and promote a shared identity with police officers, thereby attracting a larger and more potentially diverse pool of candidates.
An Initial Genome Editing Toolset for Caldimonas thermodepolymerans, the First Model of Thermophilic Polyhydroxyalkanoates Producer
Authors: Grybchuk‐Ieremenko Anastasiia , Lipovská Kristýna , Kouřilová Xenie , Obruča Stanislav , Dvořák Pavel 
Published: February 2025

ABSTRACTThe limited number of well‐characterised model bacteria cannot address all the challenges in a circular bioeconomy. Therefore, there is a growing demand for new production strains with enhanced resistance to extreme conditions, versatile metabolic capabilities and the ability to utilise cost‐effective renewable resources while efficiently generating attractive biobased products. Particular thermophilic microorganisms fulfil these requirements. Non‐virulent Gram‐negative Caldimonas thermodepolymerans DSM15344 is one such attractive thermophile that efficiently converts a spectrum of plant biomass sugars into high quantities of polyhydroxyalkanoates (PHA)—a fully biodegradable substitutes for synthetic plastics. However, to enhance its biotechnological potential, the bacterium needs to be ‘domesticated’. In this study, we established effective homologous recombination and transposon‐based genome editing systems for C. thermodepolymerans. By optimising the electroporation protocol and refining counterselection methods, we achieved significant improvements in genetic manipulation and constructed the AI01 chassis strain with improved transformation efficiency and a ΔphaC mutant that will be used to study the importance of PHA synthesis in Caldimonas. The advances described herein highlight the need for tailored approaches when working with thermophilic bacteria and provide a springboard for further genetic and metabolic engineering of C. thermodepolymerans, which can be considered the first model of thermophilic PHA producer.