bluuebunny/crossref_metadata_2025_split huggingface dataset:
High‐throughput behavioral phenotyping of tiny arthropods: Chemosensory traits in a mesostigmatic hematophagous mite
Authors: Masier Stefano , Taudière Adrien , Roy Laurent J. M. , Carrasco David , Barnagaud Jean‐Yves , Planchon Camille , Soulié Anne‐Sophie , Sleeckx Nathalie , Roy Lise 
Published: September 2022

AbstractPest management using attractive and/or repellent semiochemicals is a key alternative to synthetic insecticides. Its implementation requires a good understanding of the intra‐ and interspecific chemical interactions of arthropod pests, their interactions with their abiotic environment, as well as their evolutionary dynamics. Although mites include many pest species and biocontrol agents of economic importance in agriculture, their chemical ecology is largely understudied compared to insects. We developed a high‐throughput ethomics system to analyze these small arthropods and conducted a study onDermanyssus gallinae, a problematic poultry parasite in the egg industry. Our purpose was to elucidate the role played by host‐derived odorants (synthetic kairomone) and conspecific odorants (mite body odors) inD. gallinae. After validating our nanocomputer controlled olfactometric system with volatile semiochemicals of known biological activity, we characterized response traits to kairomonal and/or pheromonal volatile blends in mites from different populations. We were able to accurately characterize the repulsion or attraction behaviors in >1000 individual specimens in a standardized way. Our results confirm the presence of a volatile aggregation pheromone emitted byD. gallinaeand bring new elements to the effect of odor source presentation. Our results also confirm the attractive effect onDermanyssus gallinaeof a blend of volatile compounds contained in hen odor, while highlighting a repellent effect at high concentration. Significant interindividual and interpopulation variation was noted particularly in responses to synthetic kairomone. This information lays a valuable foundation for further exploring the emergence risk of resistance to semiochemicals.
Locally common graphs
Authors: Csóka Endre , Hubai Tamás , Lovász László 
Published: September 2022

AbstractGoodman proved that the sum of the number of triangles in a graph on nodes and its complement is at least ; in other words, this sum is minimized, asymptotically, by a random graph with edge density 1/2. Erdős conjectured that a similar inequality will hold for in place of , but this was disproved by Thomason. But an analogous statement does hold for some other graphs, which are called common graphs. Characterization of common graphs seems, however, out of reach. Franek and Rödl proved that is common in a weaker, local sense. Using the language of graph limits, we study two versions of locally common graphs. We sharpen a result of Jagger, Štovíček and Thomason by showing that no graph containing can be locally common, but prove that all such graphs are weakly locally common. We also show that not all connected graphs are weakly locally common.
Clinical significance of preoperative antibiotic use in mild to moderate acute inflammatory gallbladder disease: A randomized controlled trial
Authors: Park Sung Eun , Choi Ho Jung , You Young Kyoung , Hong Tae Ho 
Published: September 2022

AbstractBackgroundMany patients receive empirical antibiotics for the prevention of postoperative infectious complications following cholecystectomy due to acute cholecystitis (AC). The purpose of this study was to investigate the clinical significance of preoperative antibiotics in mild to moderate AC patients undergoing emergency laparoscopic cholecystectomy.MethodsThis was a double‐blind, placebo‐controlled, randomized study. Patients with AC eligible for emergency laparoscopic cholecystectomy were randomly assigned to an antibiotic or a placebo group. Clinical outcomes including infectious complications were reviewed.ResultsAn imputed per‐protocol analysis of 234 patients showed that the postoperative infection rate was 8.6% (10 of 116 patients) in the antibiotic group and 7.6% (9 of 118 patients) in the placebo group (absolute difference, 1%; 95% CI: −8.1% to 6.1%; P = .815). Based on a noninferiority margin of 10%, the lack of preoperative antibiotic treatment was not associated with worse clinical outcomes than antibiotic treatment. Surgical site infection was the most common complication among the infectious complications, and there was no significant difference between the two groups (7.8% in the antibiotic group vs 7.6%, in the placebo group, P = .53).ConclusionsThe absence of prophylactic antibiotics has no significant impact on the incidence of infectious complications in mild to moderated AC.
Out of the closet? Reconstructing the personal life of pioneering sex researcher Katharine Bement Davis
Authors: Jabour Anya 
Published: September 2022

AbstractKatharine B. Davis was an important progressive‐era figure, a pioneering professional, an innovative penologist, and an iconoclastic sexologist. Although scholars have long been aware of Davis's tolerant attitude toward same‐sex relationships at the New York State Female Reformatory at Bedford Hills, where she was Superintendent from 1901 to 1913, and her open discussion of same‐sex attraction in her study of “normal” women's sexuality, published in 1929, little has been known about Davis's personal life. Thus, it was a feminist biographer's dream come true to gain access to what Davis called her “autobiographical biography,” the never‐finished, never‐published, story of her life. Or so I thought. As it turns out, my quest to understand Davis's personal life and how it informed her professional trajectory has been a bit more complicated.
Predicting Early Vestibular and Motor Function in Congenital Cytomegalovirus Infection
Authors: Dhondt Cleo , Maes Leen , Martens Sarie , Vanaudenaerde Saartje , Rombaut Lotte , Sucaet Marieke , Keymeulen Annelies , Van Hoecke Helen , De Leenheer Els , Dhooge Ingeborg 
Published: September 2022

ObjectivesCongenital cytomegalovirus (cCMV) can affect vestibular function, which is an important cornerstone for early motor development. This study aims to identify risk factors for early vestibular dysfunction with severe repercussions on the motor outcome.MethodsThis prospective cohort study included 169 cCMV‐patients with complete vestibular assessment (lateral video Head Impulse Test and cervical Vestibular Evoked Myogenic Potentials) before the age of 18 months (mean 8.9, standard deviation 3.27 months). Motor results using the Alberta Infant Motor Scale were collected in 152 of these patients. Logistic and linear regression models were applied to identify risk factors for the vestibular and motor outcomes, respectively.ResultsThe odds of developing early vestibular dysfunction were 6 times higher in patients presenting with hearing loss at birth compared to those born with normal hearing (p = .002). Within the latter group, significant predictors for vestibular dysfunction were (delayed‐onset) hearing impairment at the time of vestibular testing (p = .003) and the presence of periventricular cysts on magnetic resonance imaging (p = .005). Remarkably, none of the patients infected during the third trimester of pregnancy (n = 14) developed early vestibular dysfunction. On average, vestibular‐impaired patients had a z‐score on the Alberta Infant Motor Scale that was 1.17 points lower than patients without vestibular deficit (p < .001).ConclusionEarly vestibular loss can have a significant adverse effect on motor development. Hearing and cranial imaging findings could facilitate the widespread implementation of a (targeted) vestibular assessment approach in the cCMV‐population.Level of Evidence3 Laryngoscope, 133:1757–1765, 2023
Body Dysmorphic Disorder in Adult Patients With an Orofacial Cleft: An Unseen Psychological Burden
Authors: Stepp Wesley H. , Stein Eva J. , Canfarotta Michael W. , Wood Jeyhan , Vandoros Eva , Stein Margot , Daniel Renie , Shockley William W. , Clark Joseph Madison , Drake Amelia F. 
Published: September 2022

ObjectivesFacial dysmorphic disorder (FDD), a variant of body dysmorphic disorder, occurs when individuals are preoccupied with perceived defects in their facial appearance. Cleft lip and/or palate (CL/P) requires many clinical interventions and has significant psychological impacts on a patient's perception of appearance. This study identified psychological burdens related to living as an adult with CL/P and characterizes the degree of FDD symptoms in an adult craniofacial population.MethodsThis was a prospective, single‐center, cross‐sectional case–control study using semi‐structured interviews and symptom assessments at a university‐based craniofacial center. Patients without CL/P undergoing non‐cosmetic facial surgery were recruited as controls (n = 20). Patients with an orofacial cleft (n = 30) were recruited from medical and dental providers at the University of North Carolina. Body Dysmorphic Disorder‐Yale Brown Obsessive Compulsive Scale (BBD‐YBOCS) scores were collected from a control population and patients with CL/P to assess FDD severity.ResultsDemographic factors such age, biological sex, and ethnicity had no significant impact on FDD symptom scores. Patient with CL/P were more likely to have significant FDD symptoms (BDD‐YBOCS greater than 16) than patients without CL/P (OR 10.5, CI95 2.7–41.1), and had a mean difference in FDD symptoms scores of 10.04 (p < 0.0001; CI95 5.5–14.6). Patients with CL/P seen by a mental health provider in the past 3 months had 3‐fold lower overall FDD symptom scores (OR 0.081; CI95 0.0085–0.77).ConclusionsAdults with CL/P would benefit from treatment for cleft‐specific needs and psychological support as they face unique stressors related to their appearance, including an increase in FDD‐associated symptoms. This study emphasizes the importance of recognizing psychological symptoms and providing ongoing multidisciplinary care to adults with CL/P.Level of Evidence3; Individual case–control study Laryngoscope, 133:818–821, 2023
The Impact of Polymer Architecture on Polyion Complex (PIC) Micelles: When Topology Matters (and When It Doesn't)
Authors: Li Chendan , van Ravensteijn Bas G. P. , Stuart Martien A. Cohen , Magana José Rodrigo , Voets Ilja K. 
Published: September 2022

AbstractThe influence of homopolymer architecture on the properties of polyion complex micelles is reported. Using a combination of dynamic and static light scattering, the authors show how the architecture is only relevant in kinetically trapped states of micelles formed by the electrostatic assembly of poly(N‐isopropyl acrylamide‐block‐styrene sulfonate) (p(NIPAM‐b‐SS) and linear, 4‐arm, 8‐arm star quaternized poly(dimethyl amino ethyl acrylate) (PDMAEA) homopolymers or poly(amidoamine) (PAMAM) dendrimers. Interestingly, the micellar size and the aggregation number in these kinetically arrested states follow a clear trend with the number of arms but differ in the case of dendrimers possibly due to the distinct chemical nature of the monomers. The authors show that if the micelles are prepared in a weak polyelectrolyte pairing regime (i.e., high ionic strength), they all converge into similar structures. The presented findings represent a new way of controlling the properties of polyion complex micelles through kinetically trapped states.
A Mechanochemically Active Metal‐Organic Framework (MOF) Based on Cu‐Bis‐NHC‐Linkers: Synthesis and Mechano‐Catalytic Activation
Authors: Shinde Kshitij Sanjay , Michael Philipp , Fuhrmann Daniel , Binder Wolfgang H. 
Published: September 2022

AbstractPorous coordination polymers, more commonly known as metal‐organic frameworks (MOFs), are constructed from metal ions and organic linkers which form a robust network‐like structure similar to very dense polymeric networks. In this work the mechanochemical activation of a MOF is reported, based on the previous results on the mechanochemical activation of latent Cu(I)‐based N‐heterocyclic carbenes (NHCs) embedded covalently in various polymers. A mechanochemically triggered copper‐catalyzed azide‐alkyne cycloaddition (CuAAC) is achieved using an NHC‐MOF, constructed from 1,3‐bis(4‐carboxyphenyl)imidazolium chloride as organic linker. A Cu(I) bis(NHC) is embedded into the MOF analogous to ([Zn4O{Cu(L)2}2]) using the organic bis‐NHC ligand, in turn generating a largely amorphous MOF. On activation by ultrasound, the MOF containing 4.66% of Cu shows mechanochemical activation to obtain Cu(I), subsequently triggering a CuAAC with conversions up to 26.5% as monitored by the reaction of benzylazide and phenylacetylene to yield 1‐benzyl‐4‐phenyl‐1H‐1,2,3‐triazole over a period of 20 sonication cycles. With this knowledge in solution, there are prospects to transfer this mechanochemical activity into polymer networks with embedded MOFs to detect stress in hybrid materials.
Addition–Fragmentation Ring‐Opening Polymerization of Bio‐Based Thiocarbonyl l‐Lactide for Dual Degradable Vinyl Copolymers
Authors: Kamiki Ryoya , Kubo Tomohiro , Satoh Kotaro 
Published: September 2022

AbstractThis study is designed to synthesize novel degradable polymers by radical addition–fragmentation ring‐opening copolymerization of bio‐based thiocarbonyl compounds with various vinyl monomers. Thiocarbonyl l‐lactide is capable of radical copolymerization with acrylates and styrene via radical addition to the carbon–sulfur double bonds followed by ring‐opening as well as controlled copolymerization in conjunction with the reversible addition–fragmentation chain transfer (RAFT) process. The obtained polymers possess ring‐opened thioester and ring‐retained thioacetal functionalities in the backbone, both of which could be cleaved under appropriate conditions with different chemical stimuli.
Analyzing RNA posttranscriptional modifications to decipher the epitranscriptomic code
Authors: Deng L. , Kumar J. , Rose R. , McIntyre W. , Fabris Daniele 
Published: September 2022

AbstractThe discovery of RNA silencing has revealed that non‐protein‐coding sequences (ncRNAs) can cover essential roles in regulatory networks and their malfunction may result in severe consequences on human health. These findings have prompted a general reassessment of the significance of RNA as a key player in cellular processes. This reassessment, however, will not be complete without a greater understanding of the distribution and function of the over 170 variants of the canonical ribonucleotides, which contribute to the breathtaking structural diversity of natural RNA. This review surveys the analytical approaches employed for the identification, characterization, and detection of RNA posttranscriptional modifications (rPTMs). The merits of analyzing individual units after exhaustive hydrolysis of the initial biopolymer are outlined together with those of identifying their position in the sequence of parent strands. Approaches based on next generation sequencing and mass spectrometry technologies are covered in depth to provide a comprehensive view of their respective merits. Deciphering the epitranscriptomic code will require not only mapping the location of rPTMs in the various classes of RNAs, but also assessing the variations of expression levels under different experimental conditions. The fact that no individual platform is currently capable of meeting all such demands implies that it will be essential to capitalize on complementary approaches to obtain the desired information. For this reason, the review strived to cover the broadest possible range of techniques to provide readers with the fundamental elements necessary to make informed choices and design the most effective possible strategy to accomplish the task at hand.
De novo biosynthesis of α‐aminoadipate via multi‐strategy metabolic engineering in Escherichia coli
Authors: Zhang Yang , Liu Meng , Cai Bingqi , He Keqin , Wang Meng , Chen Biqiang , Tan Tianwei 
Published: September 2022

AbstractAs a non‐protein amino acid, α‐aminoadipate is used in the fields of medicine, chemical engineering, food science, and others. For example, α‐aminoadipate is an important precursor for the production of β‐lactam antibiotics. Currently, the synthesis of α‐aminoadipate depends on chemical catalysis that has the disadvantages of high cost, low yield, and serious pollution. In this study, we construct a biosynthesis pathway of α‐aminoadipate in Escherichia coli using lysine as a precursor. In addition, we regulate the cell metabolism to improve the titer of α‐aminoadipate via multi‐strategy metabolic engineering. First, a novel synthetic pathway was constructed to realize de novo synthesis of α‐aminoadipate with titers of 82 mg/L. Second, the key enzymes involved in enhancing precursor synthesis were overexpressed and the CO2 fixation process was introduced, and these led to 80% and 34% increases in the α‐aminoadipate concentration, reaching 147 and 110 mg/L, respectively. Third, cofactor regulation was used to maintain the coupling balance of material and energy, with the intracellular α‐aminoadipate concentration reaching 140 mg/L. Fourth, the weakening of the synthesis of acetic acid was used to strengthen the synthesis of α‐aminoadipate, and this resulted in the enhancement of the α‐aminoadipate concentration by 2.2 times, reaching 263 mg/L. Finally, combination optimization was used to promote the production of α‐aminoadipate. The titers of α‐aminoadipate reached 368 mg/L (strain EcN11#) and 415 mg/L (strain EcN11##), which was 3.5 and 4 times higher than that of the parent strain. With these efforts, 1.54 g/L of α‐aminoadipate was produced under fed‐batch conditions by strain EcN11#. This study is the first to present the effective biosynthesis of α‐aminoadipate in E. coli using multi‐strategy metabolic engineering.
The Gray Area of Freezing of Gait Annotation: A Guideline and Open‐Source Practical Tool
Authors: Cockx Helena , Klaver Emilie , Tjepkema‐Cloostermans Marleen , van Wezel Richard , Nonnekes Jorik 
Published: September 2022

ABSTRACTBackgroundFreezing of gait, a disabling episodic symptom, is difficult to assess as the exact begin‐ and endpoint of an episode is not easy to specify. This hampers scientific and clinical progress. The current golden standard is video annotation by two independent raters. However, the comparison of the two ratings gives rise to non‐overlapping, gray areas.ObjectiveTo provide a guideline for dealing with these gray areas.Methods/ResultsWe propose a standardized procedure for handling the gray areas based on two parameters, the tolerance and correction parameter. Furthermore, we recommend the use of positive agreement, negative agreement, and prevalence index to report interrater agreement instead of the commonly used intraclass correlation coefficient or Cohen's kappa. This theoretical guideline was implemented in an open‐source practical tool, FOGtool (https://github.com/helenacockx/FOGtool).ConclusionThis paper aims to contribute to the standardization of freezing of gait assessment, thereby improving data sharing procedures and replicability of study results.
Reproductive health of the sow during puerperium
Authors: Björkman Stefan , Kauffold Johannes , Kaiser Marianne Ørnstrup 
Published: September 2022

AbstractThe modern hyperprolific sow is susceptible to metabolic disease and chronic inflammation. The most sensitive phase is parturition, when the sow experiences systemic inflammation and stress, and major changes in metabolism and endocrinology. Resolution of inflammation and stress needs to happen quickly to ensure good reproductive health during puerperium. If the sow fails to adapt to these changes, puerperal disease may occur. The economically most important puerperal disease complex is the postpartum dysgalactia syndrome (PPDS). Other puerperal diseases include infections of the urogenital tract. Diagnosis of PPDS and urogenital disease on‐farm is challenging but several diagnostic methods, including clinical examination, behavioral observations, ultrasonography and biomarkers are available. Ultrasonography is an excellent tool for monitoring the health of the urogenital tract, the mammary gland, and uterine involution and guide further diagnostic interventions. Biomarkers such as Chromogranin A, tumor necrosis factor‐α, and interleukin‐6 represent promising tools to monitor general health and the systemic state of inflammation and oxidative stress of the sow. Nonsteroidal anti‐inflammatory drugs, dopamine antagonists, and oxytocin are promising to address the symptoms of PPDS. Reducing of stress, improving nutrition and intestinal health, and supporting animal welfare‐friendly husbandry help in the prevention of PPDS.
Alterations in insulin‐like growth factor system in spinal muscular atrophy
Authors: Kaymaz Ayse Yesbek , Bal Sevgi Kostel , Bora Gamze , Talim Beril , Ozon Alev , Alikasifoglu Ayfer , Topaloglu Haluk , Yurter Hayat Erdem 
Published: September 2022

AbstractIntroduction/AimsSpinal muscular atrophy (SMA) is an inherited neuromuscular disease caused by survival motor neuron (SMN) protein deficiency. Insulin‐like growth factor‐I (IGF‐I) is a myotrophic and neurotrophic factor that has been reported to be dysregulated in in vivo SMA model systems. However, detailed analyses of the IGF‐I system in SMA patients are missing. In this study, we analyzed the components of the IGF‐I system in serum and archived skeletal muscle biopsies of SMA patients.MethodsSerum IGF‐I, IGF binding protein (IGFBP)‐3, and IGFBP‐5 levels were analyzed in 11 SMA patients and 13 healthy children by immunoradiometric and enzyme‐linked immunosorbent assays. The expression of IGF‐I, IGF‐I receptor, and IGFBP‐5 proteins was investigated by immunofluorescence analysis in the archived skeletal muscle biopsies of nine SMA patients, six patients with non‐SMA‐related neuromuscular disease and atrophic fibers in muscle biopsy, and four controls.ResultsA significant decrease in IGF‐I levels (mean ± SD: −1.39 ± 1.46 vs. 0.017 ± 0.83, p = .02) and increase in IGFBP‐5 levels (mean ± SD: 2358.5 ± 1617.4 ng/mL vs. 1003.4 ± 274.3 ng/mL, p = .03) were detected in serum samples of SMA patients compared to healthy controls. Increased expression of IGF‐I, IGF‐I receptor, and IGFBP‐5 was detected in skeletal muscle biopsies of SMA patients and non‐SMA neuromuscular diseases, indicating atrophy‐specific alterations in the pathway.DiscussionOur findings suggested that the components of the IGF‐I system are altered in SMA patients at both the systemic and tissue‐specific levels.