bluuebunny/crossref_metadata_2025_split huggingface dataset:
Data Analysis and Graphical observations of real-time RT-PCR of Hepatitis C Virus to determine the range of qualitative assays and to differentiate it from quantitative assays
Authors: Joseph Ann M. , Yathi Krishna K. , Jacob Jose 
Published: July 2022

Abstract
Hepatitis C Virus (HCV) load in plasma were often below the validated quantitative RT-PCR (RT-qPCR) calibration plot concentrations. In this report, data analysis of RT-PCR amplification plots and calibration plots were done to define the performance characteristics of the higher Cq range of qualitative RT-PCR and to differentiate it from RT-qPCR. This is the first report to establish the decision criteria for validation of qualitative real-time HCV RT-PCR assays. Patients reactive for anti-HCV antibody screening assays or exposed to HCV infection were selected for RT-PCR assay done with RNA isolated from plasma. Normalised fluorescence intensity (100%) and slope (1.65–1.50) of the sigmoid fluorescence amplification plots were of acceptable performance for validated quantitative calibrators (10,000–10 IU/µl), but were decreased for diluted calibrators and for patients’ sample with Cq greater than that of the 10 IU/µl validated calibrator (Cq > 33.2). Calibration plot of validated calibrators had slope, efficiency and sensitivity of -3.471, 94% and 36.404, respectively, but the diluted calibrators showed decreased performance of -2.305, 171% and 35.52, respectively. Log-transformed exponential amplification plots analysed by LinRegPCR software, showed acceptable performance at the validated calibrator concentrations and decreased performance with diluted calibrators and patients’ sample with Cq > 33.2. The four-parameter validated sigmoid fluorescence amplification plot of qualitative RT-PCR Cq range was from 33.2 to 39.9. The 10 IU/µl calibrator was the reference point to differentiate between qualitative and quantitative RT-PCR. A systematic decision-criteria had been developed for this purpose from the data analysis of graphical observations, four-parameter sigmoid curve fitting model, exponential amplifications of individual RT-PCR plots and calibration plots.
Effectiveness of grey and green engineered solutions for protecting the low-lying muddy coast of the Chao Phraya Delta, Thailand
Authors: Charoenlerkthawin Warit , Bidorn Komkrit , Burnett William C. , Sasaki Jun , Panneerselvam Balamurugan , Bidorn Butsawan 
Published: July 2022

Abstract
Coastal protection measures can be categorized into grey and green solutions in terms of their ecosystem impacts. As the use of grey solutions has become a serious issue due to environmental consequences during the last few decades, green/nature-based solutions have become prioritized. This study evaluates the effectiveness of grey and green solutions applied along the eastern Chao Phraya Delta (ECPD) based on historical shoreline change analysis and coastal observations using Light Detection and Ranging (LiDAR) technology. The results from shoreline analysis indicate that nearshore breakwaters installed 100–250 m from the shoreline have successfully reclaimed the coastline with a sedimentation rate of 17–23 cm/y. Meanwhile, sand-sausage-submerged breakwaters were ineffective at stabilizing the coastline during 2002–2010 due to land subsidence. With a low subsidence rate, the rubble-mound-submerged breakwaters can reduce the shoreline retreat rate with a vertical deposition rate of about 5 cm/y. In contrast, use of a bamboo fence, a green solution widely used along muddy coasts, traps sediment at a rate of less than 1.3 cm/y and typically lasts only for 2–3 years after installation. Decomposed bamboo causes environmental degradation so local communities disapprove of the approach. Results reveal that grey solutions are more effective for stabilizing the ECPD coastline and result in less coastal environmental impact than the nature-based solution using a bamboo fence.
Gene Expression Profiling of Fibroepithelial Lesions of the Breast
Authors: Li Xiaomo , Vail Eric , Maluf Horacio , Chaum Manita , Lownik Joseph , Che Mingtian , Giuliano Armando , Dadmanesh Farnaz 
Published: July 2022

Abstract
Fibroepithelial lesions of the breast (FELs) are a heterogeneous group of neoplasms exhibiting a histologic spectrum ranging from fibroadenomas (FAs) to malignant phyllodes tumors (PTs). Despite published histologic criteria for their classification, it is common for such lesions to exhibit overlapping features, leading to subjective interpretation and inter-observer disagreements in histologic diagnosis. Therefore, there is a need for a more objective diagnostic modality to aid in the accurate classification of these lesions and to guide appropriate clinical management. In this study, the expression of 750 tumor-related genes was measured in a cohort of 34 FELs (5 FAs, 9 cellular FAs, 9 benign PTs, 7 borderline PTs, and 4 malignant PTs). Differentially expressed gene analysis, gene set analysis, pathway analysis, and cell type analysis were performed. Genes involved in matrix remodeling and metastasis (e.g., MMP9, SPP1, COL11A1), angiogenesis (VEGFA, ITGAV, NFIL3, FDFR1, CCND2), hypoxia (ENO1, HK1, CYBB, HK2), metabolic stress (e.g., UBE2C, CDKN2A, FBP1), cell proliferation (e.g., CENPF, CCNB1), PI3K-Akt pathway (e.g., ITGB3, NRAS) were highly expressed in malignant PTs and less expressed in borderline PTs, benign PTs, cellular FAs, and FAs. The overall gene expression profiles of benign PTs, cellular FAs, and FAs were very similar. Although a slight difference was observed between borderline and benign PTs, a higher degree of difference was observed between borderline and malignant PTs. Additionally, the macrophage cell abundance scores and CCL5 were significantly higher in malignant PTs compared with all other groups. Our results suggest that the gene expression profiling-based approach could lead to further stratification of FELs and may provide clinically useful biological and pathophysiological information to improve the existing histologic diagnostic algorithm.
Screening of potential hub genes and key pathways associated with breast cancer by bioinformatics tools.
Authors: Oumeddour Abdelkader 
Published: July 2022

Abstract
Purpose Breast cancer (BC) remains the main leading cause of cancer death in women worldwide. Developing new-targeted therapies remains an area of growing interest that may improve patient survival. Therefore, the aim of this study was to analyze the differentially expressed genes (DEGs) involved in BC development and progression to better predict biomarkers, which may be used as targeted therapies.Methods DEGs were picked out from three gene expression profiles GSE55715, GSE124646 and GSE87049 using GEO2R tool and Venn diagram software. Then, these DEGs were analyzed by different bioinformatics tools, including gene ontology (GO) and pathway enrichment analysis, protein-protein interaction (PPI), survival analysis, expression validation in BC tissues, transcription factor identification and immune cell infiltration, to explore key genes involved in BC progression.Results A total of 146 DEGs were identified in the three datasets, including 60 up-regulated genes were enriched in cell cycle, oocyte meiosis, progesterone-mediated oocyte maturation, p53 signaling pathway, HTLV-I infection and Hepatitis B, while 86 down-regulated genes were enriched mainly in phenylalanine metabolism, tyrosine metabolism, melanogenesis, mineral absorption, TNF signaling pathway and pathways in cancer. Furthermore, ten hub genes were obtained: BUB1, CDK1, HMMR, MAD2L1, CEP55, AURKA, CCNB2, TPX2, MELK and KIF20A. The overexpression levels of hub genes, except CDK1, were associated with the poor survival in BC, and regulated by several transcription factors (TFs) involved in DNA binding activity and transcription regulation. The infiltration levels of immune cells were positively correlated with these hub genes, especially macrophages and CD4+ T cells.Conclusion In conclusion, this study provides reliable molecular biomarkers that can become potential therapeutic targets in BC treatment.
Pricing Design for a Low Carbon Economy
Authors: L Alberto Lamadrid , Shi Xin , Zuluaga Luis 
Published: July 2022

Abstract
We study the problem of deriving financially adequate prices for trading a perishable commodity under a centralized auction with environmental considerations. Our model is inspired by electricity markets, with conventional producers having byproduct emissions; and environmentally clean producers without emissions but subject to weather uncertainty. We derive a new market clearing pricing scheme that takes into account the marginal costs associated with operations at maximum capabilities. Our pricing scheme guarantees revenue adequacy for the market administrator, cost recovery for all producers, and allows to analyze the effects of environmental limitations. We illustrate our model calibrating to data from U.S. markets.
We show how our design alters the participation of conventional and clean producers, and provides revenues to compensate the capital costs required to decarbonize production portfolios, and decrease the emissions produced by the market (system) being considered.
On n-quasi-(m,q)-isometric operators on a Banach space
Authors: Gherairi Khadija 
Published: July 2022

Abstract
In this paper, we introduce the class of n-quasi-m-isometric op-erators on a Banach space. Such a class seems to be a natural generalizationof m-isometric operators on Banach spaces and of n-quasi-m-isometric oper-ators on Hilbert spaces. We start by giving some of their elementary prop-erties, studying the products and the power of such operators. Next, we willexplain the relation between C 0 -semigroups, n-quasi-(m, q)-isometric operatorsand (m, q)-isometric operators. Finally, we focus on the dynamic of an n-quasi-m-isometry. More precisely, we prove a result characterizing the supercyclicityof such a class.
SIMD vectorization for simultaneous solution of locally varying linear systems with multiple right hand sides
Authors: Kühn Martin J. , Holke Johannes , Lutz Annette , Thies Jonas , Röhrig-Zöllner Melven , Bleh Alexander , Backhaus Jan , Basermann Achim 
Published: July 2022

Abstract
Developments in numerical simulation of flows and high performance computing influence one another. More detailed simulation methods create a permanent need for more computational power, while new hardware developments often require changes to the software to exploit new hardware features.This dependency is very pronounced in the case of vector-units which are featured by all modern processors to increase their numerical throughput but require vectorization of the software to be used efficiently. We study the vectorization of a simulation method that exhibits an inherent level of vector-parallelism. This is of particular interest as SIMD operations will hopefully be available with std::simd in a future C++ standard.The simulation method considered here results in the simultaneous solution of multiple sparse linear systems of equations which only differ by their main diagonal and right hand sides. Such structure arises in the simulation of unsteady flow in turbomachinery by means of a frequency domain approach called harmonic balance.
High expression of MAGOH is closely associated with poor prognosis of gliomas
Authors: Wu Birui , Zhang Juntao , Tan Dianhui 
Published: July 2022

Abstract
Background: MAGOH (Mago homolog, exon junction complex subunit) is a protein-coding gene，which mediates mRNA processing. Studies have shown that MAGOH promotes gastric cancer by activating the B-Raf/MEK/ERK signaling pathway. Furthermore, this gene is involved in the development of breast cancer and prostate cancer. Therefore, we hypothesized that MAGOH also affects the formation and development of gliomas. Methods: Sequential data filtering (survival analysis, independent prognostic analysis, ROC curve analysis, and clinical correlation analysis) was performed using information on gene expression and clinical data from the Chinese Glioma Genome Atlas (CGGA) microarray database. Then, bioinformatics analysis (Kaplan-Meier survival analysis, univariate Cox analysis, multivariate Cox analysis, and ROC curve analysis) using CGGA and The Cancer Genome Atlas (TCGA) microarray was used to analyze biological manifestation of MAGOH. Gene set enrichment analysis and immune infiltration analysis were performed for this gene. R language, Perl and GSEA software were used for statistical analysis and graphic processing. Results: Kaplan-Meier survival analysis, univariate Cox analysis, multivariate Cox analysis and ROC curve analysis showed that MAGOH was an independent prognostic indicator in glioma patients, and its up-regulation was closely associated with poor prognosis of glioma patients. In addition, enrichment analysis and immune infiltration analysis showed that the immune score and interstitial score were significantly related to the survival of glioma patients. Moreover, MAGOH was closely related to the immune infiltration and inflammatory response of glioma. Conclusion: MAGOH is an independent prognostic factor in glioma patients, especially in low-grade glioma patients. MAGOH may affect the survival of glioma patients by promoting the infiltration of B cells, but inhibiting infiltration of CD4+ T cells and CD8+ T cells and activating the cell metabolism of glioma. MAGOH may be an important biomarker for the treatment of glioma.
Reconfigurable Intelligent Surface-assisted Space-Time Shift Keying
Authors: Liu jiayu , Chen Dongxiao , Bian lina , Xu xiaoting , Zhou yueru , jing xiaoping 
Published: July 2022

Abstract
Reconfigurable Intelligent Surface (RIS), which improves the performance of wireless communication systems by controlling the reflection/scattering characteristics of incident waves without increasing energy consumption, appears as a promising candidate for future wireless communication systems. In this paper, a RIS-based space-time shift keying (STSK) scheme is proposed. On the one hand, the reflection phase shifts at RIS are optimized by the channel gain maximization criterion to obtain better RIS-STSK performance; on the other hand, the theoretical average bit error probability (ABEP) of the RIS-STSK scheme is derived using a semi-analytical probabilistic approach. In addition, for improving the performance gain of the RIS-STSK scheme, an improved genetic algorithm is designed to assist the optimization of the reflection phase shifts at RIS. Simulation results show that the proposed RIS-STSK scheme performs better than the STSK scheme, and the proposed improved genetic algorithm can significantly enhance the performance gain of RIS-STSK.
The Control Strategy of Mixed Platoon Based on Vehicle Grouping with Abnormal Communication in Two-Lane Highway
Authors: Jin Shuang , Jiang Shixin , Yang Xiangli 
Published: July 2022

Abstract
The mixed platoon including human driving vehicles (HVs) and connected automated vehicles (CAVs) are becoming more common. In order to alleviate the traffic congestion of highway, we present a control strategy of mixed platoon based on vehicle grouping with abnormal communication for two-lane. First, we grouped the vehicles on the road into many small platoons according to the spatial location of the CAVs, and then proposed the CAVs control algorithm under the mixed platoon. Based on this, combined with the vehicle dynamics characteristics, we analyzed the string stability of the mixed platoon. In addition, by considering the abnormal communication of CAVs during driving, we propose a control strategy for vehicle split and vehicle formation in the two-lane situation. Through VISSIM verification, it can be seen that the method proposed in this paper has a significant effect on the improvement of mixed traffic efficiency.
Physical development of adolescent boys studying in cadet school
Authors: Balashova E. A. , Mazur L. I. , Shadrina I. L. 
Published: July 2022

Introduction. According to the results of available studies, health status of cadets is often higher than in general population, however, data shows unfavorable trends in the physical development of cadet boys.Aim. Analysis of cadets' physical development.Materials and methods. 122 cadet boys aged 12-15 were included in the study using the continuous sampling method. All children underwent anthropometry, bioimpedance analysis and measurement of the functional state of the body. The results were evaluated in accordance with centile chart.Results and discussion. The majority of children (80 - 65.6%) had 1st health group. The most common pathology was refraction disorders (14 - 11.5%) and urological pathology (9 - 7.4%). Height of the majority of the examined boys was in the average values, however, short stature was found out in 4 (3.3%) cases. According to BMI, 4.9% (6) of children were underweight, 32.0% (39) - overweight and 4.1% (5) had obesity. According to percentage of fat mass by bioimpedance analysis, 4.1% (5) were underweight, 27.0% (33) - overweight and 22.1% (27) were obese. Muscle strength values below the average were in 11.5% (14) of boys, and muscle strength by kg of weight - in 36.1% (44). Almost a third (33 - 27.0%) of children had high blood pressure, and another 10 people (8.2%) had arterial hypertension. According to the results of the Martinet-Kushelevsky test, nine children had pathological type of reaction to dosed physical activity. Low resistance to anaerobic exercise by Genchi test was found in 19.7% (24) of children. The results of the coordination test showed unsatisfactory results in the majority of children - 48.4% (59).Conclusion. Physical development of adolescent boys studying in the cadet corps was average. High percentage of boys, although less, then in general population, were overweight and obese. Despite seemingly optimal nutrition, daily routine and physical activity provided in cadet corps, 20% of children had low resistance to anaerobic exercise, low strength index and non-physiological response of the cardiovascular system to dosed physical activity.
Sore throat in children: diagnosis, treatment, prevention
Authors: Kirichenko I. M. 
Published: July 2022

Introduction. Sore throat often accompanies acute pharyngitis and tonsillitis in both children and adults. Inflammation of the posterior pharyngeal wall, acute pharyngitis, almost never occurs in isolated form in children and is often accompanied by symptoms of acute adenoiditis and / or tonsillitis.Aim. To study the effectiveness of the treatment of exacerbation of chronic non-streptococcal tonsillitis with an antiseptic herbal product in childhood.Materials and methods. We examined 58 children diagnosed with exacerbation of chronic tonsillitis for the period from June 2018 to December 2019. The age of the children ranged from 6 years 4 months up to 14 years old, the average age was 8.3 + 2.4 years, the group included 32 boys (55%) and 26 girls (45%). The duration of the disease ranged from 1 to 5.5 years. The exacerbations of chronic tonsillitis occured 3-4 times a year. The patients included in the study received the antiseptic herbal product in the form of drops at a dose of 15 drops five times/day for 7 days, and then 15 drops three times/day for another 7 days. The course of therapy was 14 days.Results. The provided course of therapy resulted in the significant positive changes overtime: reduced intensity of complaints already at the second visit, significant reduction of complaints at the third visit and actual relief of complaints by the fourth visit in the patients receiving the monotherapy with antiseptic herbal product.Conclusions. Preservation of the normal microbiome of the upper respiratory tract in children maintains effective functioning of MALT and thereby reduces the risk of the development and chronification of inflammation in the oropharynx. The antiseptic herbal medicinal product may be regarded as the alternative to antibiotic therapy in children with exacerbation of chronic tonsillitis, as it has a multifunctional effect on the mucous membranes lining upper respiratory tract, stimulates innate and adaptive immunity, and increases the resistance of mucous membranes to the pathogens.
Membentuk karakter yang kokoh melalui pendidikan hati
Authors: Suparlan Suparlan 
Published: July 2022

Pada umumnya pendidikan karakter menyentuh ranah kognitif ,afektif, dan psikomotor, belum dilandasi kesadaran spiritual dan motivasi yang tinggi, akibatnya karakter yang terbentuk merupakan karakter yang rapuh, dan kebaikan perilakunya dilatarbelakangi oleh kepentingan pribadi. Pembentukan karakter berbasis pendidikan hati menawarkan solusi pendidikan karakter yang unggul dan mampu membentuk intensitas dan konsistensi karakter yang baik. Pendidikan hati merupakan dasar dari karakter yang kokoh dan konsisten, ketika hati telah melekat pada kebaikan, maka akan menjadi sumber motif spiritual yang menggerakkan perilaku dengan kecenderungan keikhlasan, dan akan mendorong seluruh potensi psikofisik untuk berbuat baik secara konsisten. Pendidikan karakter berbasis hati dikembangkan melalui: 1) Tahap prakondisi, yaitu proses mengenali kondisi hati, mengenalkan fungsi spiritual hati, melakukan pelurusan/pengobatan hati dari berbagai penyakit hati. 2) Tahap pembentukan, yaitu proses menumbuhkan kesadaran akan pentingnya karakter, menggunakan hati dalam menentukan perilaku dan menanamkan kepekaan untuk mengambil pelajaran dari perbuatan baik. 3) Tahap perawatan, yaitu proses penyelamatan hati dari potensi kerusakan dan menghindari pengaruh karakter negatif dari lingkungan. In general, character education touches the realm of cognitive, affective, and psychomotor domains, has not been based on high spiritual awareness and motivation, as a result the character formed is a fragile character, and the goodness of behavior is motivated by personal interests. The formation of character based on heart education offers superior character education solutions and is able to form good character intensity and consistency. Heart education is the basis of a solid and consistent character, when the heart is attached to goodness, it will become a source of spiritual motives that drive behavior with a sincere tendency, and will encourage all psychophysical potentials to do good consistently. Heart-based character education is developed through: 1) Preconditioning stage, namely the process of recognizing the condition of the heart, introducing the spiritual function of the heart, performing liver straightening/treatment of various liver diseases. 2) The formation stage, namely the process of growing awareness of the importance of character, using the heart in determining behavior and instilling sensitivity to take lessons from good deeds. 3) Treatment stage, namely the process of saving the liver from potential damage and avoiding the influence of negative characters from the environment.