bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bifidobacterium animalis subsp. lactis Probio-M8 undergoes host adaptive evolution by glcU mutation and translocates to the infant’s gut via oral-/entero-mammary routes through lactation
Authors: Zhong Zhi , Tang Hai , Shen Tingting , Ma Xinwei , Zhao Feiyan , Kwok Lai-Yu , Sun Zhihong , Bilige Menghe , Zhang Heping 
Published: November 2022

Abstract
Background
Most previous studies attempting to prove the phenomenon of mother-to-infant microbiota transmission were observational, performed only at genus/species-level resolution, and relied entirely on non-culture-based methodologies, impeding interpretation.

Results
This work aimed to use a biomarker strain, Bifidobacterium animalis subsp. lactis Probio-M8 (M8), to directly evaluate the vertical transmission of maternally ingested bacteria by integrated culture-dependent/-independent methods. Our culture and metagenomics results showed that small amounts of maternally ingested bacteria could translocate to the infant gut via oral-/entero-mammary routes through lactation. Interestingly, many mother-infant-pair-recovered M8 homologous isolates exhibited high-frequency nonsynonymous mutations in a sugar transporter gene (glcU) and altered carbohydrate utilization preference/capacity compared with non-mutant isolates, suggesting that M8 underwent adaptive evolution for better survival in simple sugar-deprived lower gut environments.

Conclusions
This study presented direct and strain-level evidence of mother-to-infant bacterial transmission through lactation and provided insights into the impact of milk microbiota on infant gut colonization.
Across the deserts and sea: inter-individual variation in migration routes of south-central European barn swallows (Hirundo rustica)
Authors: Pancerasa Mattia , Ambrosini Roberto , Romano Andrea , Rubolini Diego , Winkler David W. , Casagrandi Renato 
Published: November 2022

AbstractBackgroundThe spatiotemporal organization of migratory routes of long-distance migrants results from trade-offs between minimizing the journey length anden routerisk of migration-related mortality, which may be reduced by avoiding crossing inhospitable ecological barriers. Despite flourishing avian migration research in recent decades, little is still known about inter-individual variability in migratory routes, as well as the carry-over effects of spatial and temporal features of migration on subsequent migration stages.MethodsWe reconstructed post- and pre-breeding migration routes, barrier crossing behaviour and non-breeding movements of the largest sample (N = 85) analysed to date of individual barn swallows breeding in south-central Europe, which were tracked using light-level geolocators.ResultsMost birds spent their non-breeding period in the Congo basin in a single stationary area, but a small fraction of itinerant individuals reaching South Africa was also observed. Birds generally followed a ‘clockwise loop migration pattern’, moving through the central Mediterranean and the Sahara Desert during post-breeding (north to south) migration yet switching to a more western route, along the Atlantic coast of Africa, Iberia and western Mediterranean during the pre-breeding (south to north) migration. Southward migration was straighter and less variable, while northward migration was significantly faster despite the broader detour along the Atlantic coast and Iberia. These patterns showed limited sex-related variability. The timing of different circannual events was tightly linked with previous migration stages, considerably affecting migration route and speed of subsequent movements. Indeed, individuals departing late from Africa performed straighter and faster pre-breeding migrations, partly compensating for the initial departure delays, but likely at the cost of performing riskier movements across ecological barriers.ConclusionsDifferent spatiotemporal migration strategies during post- and pre-breeding migration suggest that conditionsen routemay differ seasonally and allow for more efficient travelling along different migration corridors in either season. While highlighting patterns of inter-individual variability, our results support increasing evidence for widespread loop migration patterns among Afro-Palearctic avian migrants. Also, they suggest that carry-over effects acting across different phases of the annual cycle of migratory species can have major impacts on evolutionary processes.
The enhanced association between mutant CHMP2B and spastin is a novel pathological link between frontotemporal dementia and hereditary spastic paraplegias
Authors: Chen Yongping , Krishnan Gopinath , Parsi Sepideh , Pons Marine , Nikolaki Veroniki , Cao Lu , Xu Zuoshang , Gao Fen-Biao 
Published: November 2022

AbstractChromosome 3-linked frontotemporal dementia (FTD3) is caused by a gain-of-function mutation in CHMP2B, resulting in the production of a truncated toxic protein, CHMP2BIntron5. Loss-of-function mutations in spastin are the most common genetic cause of hereditary spastic paraplegias (HSP). How these proteins might interact with each other to drive pathology remains to be explored. Here we found that spastin binds with greater affinity to CHMP2BIntron5 than to CHMP2BWT and colocalizes with CHMP2BIntron5 in p62-positive aggregates. In cultured cells expressing CHMP2BIntron5, spastin level in the cytoplasmic soluble fraction is decreased while insoluble spastin level is increased. These pathological features of spastin are validated in brain neurons of a mouse model of FTD3. Moreover, genetic knockdown of spastin enhances CHMP2BIntron5 toxicity in a Drosophila model of FTD3, indicating the functional significance of their association. Thus, our study reveals that the enhanced association between mutant CHMP2B and spastin represents a novel potential pathological link between FTD3 and HSP.
Spoofing keystroke dynamics authentication through synthetic typing pattern extracted from screen-recorded video
Authors: Siahaan Chrisando Ryan Pardomuan , Chowanda Andry 
Published: November 2022

AbstractAs the inter-connectivity in cyberspace continues to increase exponentially, privacy and security have become two of the most concerning issues that need to be tackled in today’s state of technology. Therefore, password-based authentication should no longer be used without an additional layer of authentication, namely two-factor authentication (2FA). One of the most promising 2FA approaches is Keystroke Dynamics, which relies on the unique typing behaviour of the users. Since its discovery, Keystroke Dynamics adoption has been continuously growing to many use cases: generally to obtain access to a platform through typing behaviour similarity, into a continuous keystroke monitoring on e-learning or e-exams platforms to detect illegitimate participants or cheaters. As the adoption of Keystroke Dynamics continues to grow, so does the threats that are lurking in. This paper proposes a novel exploitation method that utilizes computer vision to extract and learn a user’s typing pattern just from a screen-recorded video that captures their typing process. By using a screen-recorded video, an attacker could eliminate the needs to inject a keylogger into the victim’s computer, thus rendering the attack easier to perform and more difficult to detect. Furthermore, the extracted typing pattern can be used to spoof a Keystroke Dynamics authentication mechanism with an evasion rate as high as 64%, a considerably alarming rate given the impact it yields if the attacks are successful, which allows an attacker to pretend, mimic, and falsely authenticate as the victim (i.e., total account takeover). This paper also shows that from a screen-recorded video, one can produce a staggering statistical similarity in keystroke timing patterns as if they used an actual keylogger, and the extracted patterns can be potentially used to spoof the Keystroke Dynamics authentication service. To the author’s best knowledge, there is no precedence of previous research that suggests this kind of attack (i.e. using video to spoof keystroke dynamics). This research can be used as the baseline for future research in this area.
Actinobacterial and fungal strain-enriched wheat straw as an effective strategy for alleviating the effect of salinity stress on soil chemical and biochemical properties
Authors: Sadeghi Elham , Ghorbani Nasrabadi Reza , Movahedi Seyed Alireza , Etesami Hassan 
Published: November 2022

Abstract
Background
Soil salinization influences the physical and chemical properties of soil and disturbs soil biodiversity. Application of wheat straw in saline soils with enhance soil fertility could mitigate the effects of salinity on soil microbial properties under laboratory conditions. However, knowledge is inadequate regarding the effects of adding enriching plant residues with beneficial organisms on soil quality in saline soil. To enhance this knowledge, an incubation experiment was performed to evaluate the effect of wheat straw (0 and 1%, w/w) enriched with microbial strains (control, Streptomyces chartreusis, Pleurotus ostreatus and a mixture of P. ostreatus and S. chartreusis.) on some soil chemical and biochemical properties under salinity stress (0, 8 and 15 dS m−1).

Results
Salinity stress led to reducing soil available phosphorus (13–23%), available potassium (5–7%), total nitrogen (3–18%). Wheat straw inoculated with S. chartreusis and P. ostreatus improved microbial respiration rate (108–305%), soil microbial biomass carbon (80–110%), microbial biomass phosphorus (50–115%), catalase activity (20–140%), urease activity (25–45%), soil organic carbon (70–100%) and dissolved organic carbon (15–20%) under all salinity levels. The effect of S. chartreusis enriched wheat straw on enzymatic and microbial properties was higher than that of wheat straw inoculated with P. ostreatus under salinity stress conditions.

Conclusions
The results of this study demonstrated that the enrichment of wheat straw with S. chartreusis and P. ostreatus act synergistically and improve soil fertility and microbial properties. It can be concluded that the combined application of wheat straw and actinobacterial and fungal strain can be an effective strategy to ameliorate soil salinity stress in agriculture.

Graphical Abstract
Changes in drug demand when a pandemic coincides with other outbreaks in a war zone country: a cross-sectional pilot study
Authors: Saleh Ebtesam A. , Haddadin Randa N. , Saleh Bassam , Elayeh Eman 
Published: November 2022

Abstract
Background
Yemen's health system has severely deteriorated due to the ongoing civil war accompanied by the COVID-19 pandemic which coincided with other outbreaks of endemic infections. Many health institutions closed due to insufficient equipment and supplies. Consequently, pharmacists became the available healthcare provider on the frontlines. This study aimed to evaluate the pattern of demand for prescription and nonprescription drugs during the pandemic based on the pharmacist's perspective in Yemen, a conflict zone country.

Methods
An online survey was developed, validated, and distributed among pharmacists. The questionnaire was composed of two sections: (1) demographic characteristics of the participants and (2) changes in the demand for different drug categories. Chi-square test and Fisher's exact test were used to find statistical associations.

Results
Responses (330) were received from pharmacists working in 12 out of 22 governorates in Yemen. During the pandemic, there was an increase in the demand for prescription drugs such as antibiotics, antimalarials, and sedatives (95%, 90%, and 71%, respectively) and an increase in the demand for nonprescription drugs such as vitamins (93%) and paracetamol (> 90%). Some of these drugs could have serious side effects if taken without medical advice, and others could result in severe effects if taken concomitantly. In addition, there was an increase in the demand for prescription drugs without a prescription, which was reported by 50% of the participants. No statistical difference was seen between the frontline districts and major cities in terms of requesting these drugs without a prescription. On the other hand, most participants (75.2%) did not attend any training or workshop during the last 6 months of conducting this survey.

Conclusion
COVID-19 has increased the demand for many prescription and nonprescription drugs, where the irrational use of these drugs may lead to devastating health effects. In war zone areas hit by a pandemic, policymakers and public health organizations should focus on training and educating pharmacists as important health care and medicine providers for the public.
Seismic velocity structure along the Sea of Japan with large events derived from seismic tomography for whole Japanese Islands including reflection survey data and NIED MOWLAS Hi-net and S-net data
Authors: Matsubara Makoto , Ishiyama Tatsuya , No Tetsuo , Uehira Kenji , Mochizuki Masashi , Kanazawa Toshihiko , Takahashi Narumi , Kamiya Shin’ichiro 
Published: November 2022

AbstractWe conducted seismic tomography for entire Japanese Islands including the Sea of Japan and the Pacific Ocean using arrival times from reflection survey as well as the routine seismic network. We successfully imaged the shallow zone along the Sea of Japan from offshore Yamagata to the Noto Peninsula by using air gun data. An extremely low-V shallow zone is imaged between Sado Island and Noto Peninsula. We also obtained detailed seismic velocity structure beneath the Pacific Ocean at depths of 20–50 km using S-net data. The 2007 Noto Peninsula, the 2007 offshore Chuetsu, and the 2019 offshore Yamagata earthquakes occurred at the boundary between high-Vp and low-Vp zones. The west side of the hypocenter of the 2019 offshore Yamagata earthquake at depths of 10–30 km has high-V corresponding to the Mogami Trough. This high-V zone passes through Awa Island and reaches Sado Basin between Sado Island and Honshu. A major rift zone in the Tohoku Arc extending from the Akita region to the Niigata region along the coast of Sea of Japan corresponds to high-V lower crust and a shallow Moho.
Graphical Abstract
Preoperative SPECT/CT + intraoperative CT fusion enabling surgical augmented reality to target sentinel lymph node in endometrial cancer
Authors: Lecointre Lise , Verde Juan , Hubele Fabrice , Salvadori Julien , Goffin Laurent , Akladios Chérif , Gallix Benoît 
Published: November 2022

Abstract
Purpose
To establish a proof-of-concept study using a phantom model to allow the fusion of preoperative single-photon emission computed tomography (SPECT) combined with computed tomography (CT), also known as SPECT/CT, with intraoperative CT, enabling the application of an augmented reality (AR) surgical guidance system for pelvic sentinel lymph node (SLN) detection in endometrial cancer patients.

Methods
A three-dimensional (3D) pelvic phantom model printed in a gelatin-based scaffold including a radiopaque pelvis, a vascular tree mimicking the iliac vessels, two 3D-printed fillable spheres representing the target pelvic sentinel lymph nodes, and a calibration board was developed. A planar with SPECT/CT lymphoscintigraphy and CT were performed independently on the model. We performed all the necessary steps to achieve the fusion between SPECT/CT and CT. Then, we performed a laparoscopy of the pelvic anatomy on the phantom model to assess in real time the overlay of the recording on the anatomical structures and AR guidance system performance.

Results
We have successfully completed all the steps needed to fuse the two imaging procedures. This allowed us to apply, in real time, our surgical guidance system with the coverage rate of the visible surface by the augmented reality surface, respectively, on the left SLN 99.48% and on the right SLN 99.42%.

Conclusion
Co-registration and real-time fusion between a preoperative SPECT/CT and intraoperative CT are feasible. The metric performance of our guidance system is excellent in relation to possible SPECT/CT and CT fusion. Based on our results, we are able to translate the technology to patients, and we initiated a clinical study to evaluate the accuracy of the AR guidance system for endometrial cancer surgery, with a correlation with indocyanine green (ICG)-based technique, representing the gold standard today in the intraoperative detection of SLN in endometrial cancers, despite various limitations.
Deep progressive learning achieves whole-body low-dose 18F-FDG PET imaging
Authors: Wang Taisong , Qiao Wenli , Wang Ying , Wang Jingyi , Lv Yang , Dong Yun , Qian Zheng , Xing Yan , Zhao Jinhua 
Published: November 2022

Abstract
Objectives
To validate a total-body PET-guided deep progressive learning reconstruction method (DPR) for low-dose 18F-FDG PET imaging.

Methods
List-mode data from the retrospective study (n = 26) were rebinned into short-duration scans and reconstructed with DPR. The standard uptake value (SUV) and tumor-to-liver ratio (TLR) in lesions and coefficient of variation (COV) in the liver in the DPR images were compared to the reference (OSEM images with full-duration data). In the prospective study, another 41 patients were injected with 1/3 of the activity based on the retrospective results. The DPR images (DPR_1/3(p)) were generated and compared with the reference (OSEM images with extended acquisition time). The SUV and COV were evaluated in three selected organs: liver, blood pool and muscle. Quantitative analyses were performed with lesion SUV and TLR, furthermore on small lesions (≤ 10 mm in diameter). Additionally, a 5-point Likert scale visual analysis was performed on the following perspectives: contrast, noise and diagnostic confidence.

Results
In the retrospective study, the DPR with one-third duration can maintain the image quality as the reference. In the prospective study, good agreement among the SUVs was observed in all selected organs. The quantitative results showed that there was no significant difference in COV between the DPR_1/3(p) group and the reference, while the visual analysis showed no significant differences in image contrast, noise and diagnostic confidence. The lesion SUVs and TLRs in the DPR_1/3(p) group were significantly enhanced compared with the reference, even for small lesions.

Conclusions
The proposed DPR method can reduce the administered activity of 18F-FDG by up to 2/3 in a real-world deployment while maintaining image quality.
The study of project cargo logistics operation: a general overview
Authors: Turbaningsih Oktaviani 
Published: November 2022

AbstractThe recent renewable energy infrastructure development trend and modular construction concept surge academic interest in the project logistics sector. The project logistics is the transportation processes for oversized cargo, critical and high value, that belong to infrastructures projects; that require specialised stowage, lifting and transporting loaded and discharged at different ports. This study aims to introduce project logistics to the public sector. The author uses the descriptive review to elaborate on the niche problem of project cargo transportation. The flow of project cargo operation from the fabrication yard to the final installation site is discussed in this paper. Planning of project cargo logistics requires an integrated planning approach that considers the following aspects total delivery cost management, end-to-end visibility, transport routing management, variability management, integrated international and domestic workflow, and technical safety consideration. The decision-making for fleet selection should be based on the optimum scenario evaluating the damage’s total logistics cost, delivery time, risks and consequences. This study can be a guide to accommodate multidiscipline studies that consider commercial, technical, and safety aspects of project logistics.
Bilateral facial nerve palsy: a rare post-dengue fever complication
Authors: Khim Ng Kim , Loh Wei Chao , Rashid Anna Misya’il Abdul , KhanKhan Abdul Hanif Yusof , Baharin Janudin , Ibrahim Azliza , Mat Liyana Najwa Inche , Sulaiman Wan Aliaa Wan , Hoo Fan Kee , Basri Hamidon 
Published: November 2022

Abstract
Background
An adolescent with recent dengue infection was presented with sudden onset of bilateral facial nerve palsy. It was a rare presentation and posed as a diagnostic challenge to all physicians.

Case presentation
A 13-year-old boy, presented with sudden onset of bilateral facial weakness. His chief complaints were noted drooping of the mouth, drooling of saliva and inability to close both eyelids. No weakness of all four limbs, ear discharges, earache or parotid gland enlargement. The only relevant significant history was recent dengue fever evident by positive dengue immunoglobulin M serology, 2 weeks prior to current presentation. During the dengue infestation, he was hospitalized for symptomatic treatment and was discharged home uneventfully. After exhausting all the available investigations and ruling out other commoner secondary causes, we finally attributed these presentations as bilateral Bell’s palsy complicated by his recent dengue infection. The mainstay of treatment was corticosteroid and rehabilitation as the usual approach towards Bell’s palsy. Our patient recovered uneventfully with no residual neurological deficit.

Conclusions
This is a rare encounter case of bilateral Bell’s palsy following dengue infection.
Translating neuroimaging changes to neuro-endophenotypes of autistic spectrum disorder: a narrative review
Authors: Sultan Sadia 
Published: November 2022

Abstract
Background
Autism-spectrum disorder is a neurodevelopmental disorder with heterogeneity in etiopathogenesis and clinical presentation. Neuroanatomical and neurophysiological abnormalities may represent neural endophenotypes for autism spectrum disorders which may help identify subgroups of patients seemingly similar in clinical presentation yet different in their pathophysiological underpinnings. Furthermore, a thorough understanding of the pathophysiology of disease can pave the way to effective treatments, prevention, and prognostic predictions. The aim of this review is to identify the predominant neural endophenotypes in autism-spectrum disorder. The evidence was researched at the following electronic databases: Pubmed, PsycINFO, Scopus, Web of Science, and EMBASE.

Results
Enlarged brain, especially frontotemporal cortices have been consistently reported by structural neuroimaging, whereas functional neuroimaging has revealed frontotemporal dysconnectivity.

Conclusions
Regrettably, many of these findings have not been consistent. Therefore, translating these findings into neural endophenotype is by far an attempt in its budding stage. The structural and functional neuroimaging changes may represent neural endophenotypes unique to autism-spectrum disorder. Despite inconsistent results, a clinically meaningful finding may require combined efforts of autism-spectrum-disorder researchers focused on different aspects of basic, genetic, neuroimaging, and clinical research.