bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐read Oxford nanopore sequencing reveals a de novo case of complex chromosomal rearrangement involving chromosomes 2, 7, and 13
Authors: Xing Lingling , Shen Ying , Wei Xiang , Luo Yuan , Yang Yan , Liu Haipeng , Liu Hongqian 
Published: June 2022

AbstractBackgroundComplex chromosomal rearrangements (CCRs) are associated with high reproductive risk, infertility, abnormalities in offspring, and recurrent miscarriage in women. It is essential to accurately characterize apparently balanced chromosome rearrangements in unaffected individuals.MethodsA CCR young couple who suffered two spontaneous abortions and underwent labor induction due to fetal chromosomal abnormalities was studied using long‐read sequencing(LRS), single‐nucleotide polymorphism (SNP) array, G‐banding karyotype analysis (550‐band resolution), and Sanger sequencing.ResultsSNP analysis of the amniotic fluid cells during the third pregnancy revealed a 9.9‐Mb duplication at 7q21.11q21.2 and a 24.8‐Mb heterozygous deletion at 13q21.1q31.1. The unaffected female partner was a carrier of a three‐way CCR [46,XX,? ins(7;13)(q21.1;q21.1q22)t(2;13)(p23;q22)]. Subsequent LRS analysis revealed the exact breakpoint locations on the derivative chromosomes and the specific method of chromosome rearrangement, indicating that the CCR carrier was a more complex structural rearrangement comprising five breakpoints. Furthermore, LRS detected an inserted fragment of chromosome 13 in chromosome 7.ConclusionsLRS is effective for analyzing the complex structural variations of the human genome and may be used to clarify the specific CCRs for effective genetic counseling and appropriate intervention.
Real‐time stereo reconstruction of intraoperative scene and registration to preoperative 3D models for augmenting surgeons' view during RAMIS
Authors: Zampokas Giorgos , Peleka Georgia , Tsiolis Konstantinos , Topalidou‐Kyniazopoulou Angeliki , Mariolis Ioannis , Tzovaras Dimitrios 
Published: June 2022

AbstractPurposeDuring minimally invansive surgery (MIS) procedures, there exists an ever‐growing/apparent need for providing computer generated visual feedback to the surgeon(s), through a visualization device. While multiple solutions have been proposed in the literature, there is limited evidence of such a system performing reliably in practice, and when it does, it is often tailored to a specific operation type. Another important aspect is regarding the usability of such systems, which typically include complicated and time‐consuming steps, and often require the assistance of specialized personnel. In this study, we propose an auxiliary visualization system for surgeons, which includes streamlined process to use preoperative data of the patient, and apply it to two different MIS cases, namely, robot‐assisted partial nephrectomy and robot‐assisted partial lateral meniscectomy.MethodsThe visualization and processing pipeline consists of an intraoperative 3D reconstruction of the surgical area, using an optimized version of the quasi‐dense method, aimed to perform with good accuracy while maintaining real‐time speed. A set of preprocessing and postprocessing techniques further contribute to the result by providing a smoother and more dense point cloud. DynamicFusion is used for the registration of the preoperative model to the intraoperative scene. Two silicon kidney phantoms and an ex‐vivo porcine meniscus are used for evaluation, representing subjects for the examined surgical cases.ResultsPerformance is evaluated qualitatively using the two datasets. The preoperative model of the subject is projected on top of the actual 2D image and also in 3D space. The model is superimposed on top of the actual physical structure it represents, and remains in the correct position throughout the experiments, even when abrupt camera movements are taking place. Finally, when deformation is introduced, the model is deformed as well, resembling the real subject's structure.ConclusionsResults demonstrate and validate the use of the presented algorithms for each separate task of the pipeline. A complete methodology to provide surgeon(s) with visual information during surgery is presented. Its operation is evaluated over two different surgical scenarios, paving the way for a single visualization methodology that can adapt and perform robustly for multiple cases, with minimal effort.
Rapid synthesis of biocompatible bilayer hydrogels via frontal polymerization
Authors: Yang Yue , Hu Jie , Liu Ji‐Dong , Qin Ying , Mao Jian , Liang Yunzheng , Wang Gefei , Shen Haixia , Wang Cai‐Feng , Chen Su 
Published: June 2022

AbstractHydrogels have been attracting much attention as smart soft materials. The exploration of methods allowing hydrogels to have anisotropic composition, structure, and properties would benefit the fabrication of smart materials with versatile multi‐functions. Here we report the rapid frontal polymerization (FP) of bilayer hydrogels possessing good water swelling, mechanical strength, self‐healing, biocompatibility, and antibacterial capabilities. The bilayer hydrogels, consisting of poly(AM‐co‐AMPS) (G‐1) and poly(AM‐co‐HPA) (G‐2) (AM, acrylamide; AMPS, 2‐acrylamide‐2‐methylpropanesulfonic acid; HPA, hydroxypropyl acrylate), are synthesized in one step within 6 minutes (min) by horizontal FP. These polymer hydrogels have hierarchical porous morphology with pore sizes of several micrometers and several hundreds of micrometers respectively, equilibrium swelling ratio up to 3371% in water, tensile strength up to 0.204 MPa, and self‐healing ability. The good cytocompatibility and low toxicity of the hydrogels are demonstrated through cell culture of L929 fibroblasts in vitro experiments. The tests of hydrogels with Escherichia coli and Staphylococcus aureus reveal their antibacterial property. The results demonstrate that biocompatible anisotropic hydrogels with versatile functions could be fabricated via the rapid and energy‐saving approach of FP.
A systematic review of the translation and validation methods used for the national comprehensive cancer network distress thermometer in non‐English speaking countries
Authors: McElroy Jane A. , Waindim Flevis , Weston Karry , Wilson Gwendolyn 
Published: June 2022

AbstractObjectiveThe National Comprehensive Cancer Network (NCCN) distress thermometer (DT) was developed as a brief screening tool for detecting distress in cancer patients. Conceptually, distress was considered a more acceptable and inclusive term than other psychological terms such as depression or anxiety. It is used extensively in the United States and other English‐speaking countries. We aim to describe the translation and validation methods of the DT and describe the recommended cutoff scores used to identify cancer patients experiencing clinically significant distress.MethodsNine bibliographic databases were searched using keywords to identify original research studies for non‐English speaking countries' cancer patients. Full articles were independently assessed for inclusion and data extraction by two authors.ResultsThe review of 4442 articles yielded 39 articles that validated the DT. We identified 20 languages in 25 countries. In all but one country, investigators were able to agree upon a word or phrase that meant distress in their native language that was subsequently validated against standardized instruments. Asian, Middle Eastern and European counties recommended cutoff score of 4 or 5 to identify clinically significant distress; however, European Union countries had a range of 2 to 7.ConclusionsUse of the translated DT with non‐English speaking cancer patients provides a simple and quick tool to identify high distress. Similar to NCCN, the cutoff score of 4 or 5 was suggested by 78% of the studies; however, more studies of different types of cancer and within and among more countries will strengthen a recommended global DT cutoff score.
Nuclear receptor HR3 mediates transcriptional regulation of chitin metabolic genes during molting in Tribolium castaneum
Authors: Kim Bo‐Eun , Choi Byungyoon , Park Woo‐Ram , Kim Yu‐Ji , Mun Seulgi , Choi Hueng‐Sik , Kim Don‐Kyu 
Published: June 2022

AbstractBACKGROUNDChitin, a major component of insect cuticles, plays a critical role in insect molting and morphogenesis. Thus, coordination of chitin remodeling during insect development requires tight transcriptional control of the chitin metabolism genes involved in chitin synthesis, assembly and degradation. However, the molecular mechanism underlying transcriptional coordination of chitin metabolism genes during beetle development is not yet completely understood.RESULTSWe cloned the full‐length cDNA encoding hormone receptor 3 (TcHR3) from Tribolium castaneum and showed a critical role of TcHR3 in modulating chitin metabolism gene expression during molting. Genome‐wide transcriptome analysis of HR3‐deficient old larvae using RNA sequencing analysis revealed a positive correlation between TcHR3 and transcription of chitin metabolism genes involved in chitin synthesis and degradation. In addition, HR3 overexpression significantly induced the gene promoter activity of N‐acetylglucosaminidase 1 (NAG1) involved in chitin degradation and UDP‐N‐acetylglucosamine pyrophosphorylase 1 (UAP1) involved in chitin synthesis. Chromatin immunoprecipitation analysis revealed that HR3 could directly bind to HR3‐response element of NAG1 and UAP1 promoters. Finally, HR3‐deficient late instar larvae and prepupae exhibited defects in larval–larval and larval–pupal molting, respectively, leading to eventual larval death because developing larvae were trapped inside the old cuticle as a result of abnormal chitin metabolism.CONCLUSIONTcHR3 is a transcriptional regulator of chitin metabolic genes for molting of T. castaneum. Controlling the molting system by TcHR3 might be a new management strategy for selective control of red flour beetle infestation. © 2022 Society of Chemical Industry.
Fructose‐Derived Levan Nanoparticles Protect Against Osteoarthritis by Directly Blocking CD44 Activation
Authors: Cho Chanmi , Lee Jin Sil , Oh Hyeryeon , Kang Li‐Jung , Hwang Yiseul , Chae Sunyoung , Lee In‐Jeong , Kim Seok Jung , Woo Hyunmin , Eyun Seong‐il , Kang Ho Chul , Choi Won Il , Yang Siyoung 
Published: June 2022

AbstractAlthough the development of several inflammatory diseases can be initiated in response to CD44 receptor signaling dysregulation, anti‐CD44 antibody therapy has been discontinued in clinical trials owing to its severe adverse effects. Moreover, biocompatible materials that block CD44 are unknown. Here, self‐assembled levan nanoparticles (LevNPs) through simple nanoprecipitation without chemical conjugation of biocompatible levan are developed. LevNPs are prepared through a simple nanoprecipitation without chemical conjugation; this results in particles with a consistently dispersed hydrodynamic diameter of approximately 230 nm. The physicochemical properties of LevNP are well‐maintained even after long‐term storage in a physiological buffer, suggesting that LevNPs are stable and useful for various biomedical applications. A protein array and an in silico LevNP‐CD44 interaction assay reveal that LevNPs specifically bind to CD44. Importantly, CD44 is highly expressed in the cartilage of patients with osteoarthritis (OA). LevNPs showed no cytotoxicity and reduced catabolic factor expression in an OA mimic in vitro. Furthermore, intra‐articular injection of LevNPs exhibited long‐term retention ability in the knee joint and protection against posttraumatic OA cartilage destruction in mice with medial meniscus destabilization–induced OA. These results show that LevNPs are biocompatible and exhibit potential as a nanotherapeutic for protecting against OA pathogenesis by directly blocking CD44.
An Ultralow Power Mixed Dimensional Heterojunction Transistor Based on the Charge Plasma pn Junction
Authors: Sul Onejae , Seo Hojun , Choi Eunsuk , Kim Sunjin , Gong Jinsil , Bang Jiyoung , Ju Hyoungbeen , Oh Sehoon , Lee Yeonsu , Sun Hyeonjeong , Kwon Minjin , Kang Kyungnam , Hong Jinki , Yang Eui‐Hyeok , Chung Yunchul , Lee Seung‐Beck 
Published: June 2022

AbstractDevelopment of a reliable doping method for 2D materials is a key issue to adopt the materials in the future microelectronic circuits and to replace the silicon, keeping the Moore's law toward the sub‐10 nm channel length. Especially hole doping is highly required, because most of the transition metal dichalcogenides (TMDC) among the 2D materials are electron‐doped by sulfur vacancies in their atomic structures. Here, hole doping of a TMDC, tungsten disulfide (WS2) using the silicon substrate as the dopant medium is demonstrated. An ultralow‐power current sourcing transistor or a gated WS2 pn diode is fabricated based on a charge plasma pn heterojunction formed between the WS2 thin‐film and heavily doped bulk silicon. An ultralow switchable output current down to 0.01 nA µm−1, an off‐state current of ≈1 × 10−14 A µm−1, a static power consumption range of  1 fW µm−1–1 pW µm−1, and an output current ratio of 103 at 0.1 V supply voltage are achieved. The charge plasma heterojunction allows a stable (less than 3% variation) output current regardless of the gate voltage once it is turned on.
The ties that bind: geopolitical motivations for economic integration
Authors: Hinz Julian 
Published: June 2022

AbstractEconomic determinants of economic integration agreements have received ample attention in the economic literature. Political motivations for such agreements have been mostly studied as functions of domestic politics or in the context of conflict. In this paper the author suggests a different narrative. Economic integration may be used as an instrument of foreign policy, where political considerations influence the choice of contracting partners. He sketches a simple model that exhibits the proposed mechanism in which abigcountry chooses between alternatives for integration in terms of economic and political welfare gains, while asmallcountry is indifferent between possible partners for integration. In the empirical part the author uses a novel dataset on political events to test the predictions of the model and finds evidence for the hypothesis that there is more to economic integration than “just trade”. Geopolitical considerations play a determining role in the choice of the contracting partner country and the depth of economic integration.
Real-time path planning for autonomous vehicle based on teaching–learning-based optimization
Authors: Sabiha Ahmed D. , Kamel Mohamed A. , Said Ehab , Hussein Wessam M. 
Published: June 2022

AbstractThis paper presents an online path planning approach for an autonomous tracked vehicle in a cluttered environment based on teaching–learning-based optimization (TLBO), considering the path smoothness, and the potential collision with the surrounding obstacles. In order to plan an efficient path that allows the vehicle to be autonomously navigated in cluttered environments, the path planning problem is solved as a multi-objective optimization problem. First, the vehicle perception is fully achieved by means of inertial measurement unit (IMU), wheels odometry, and light detection and ranging (LiDAR). In order to compensate the sensors drift to achieve more reliable data and improve the localization estimation and corrections, data fusion between the outputs of wheels odometry, LiDAR, and IMU is made through extended Kalman filter (EKF). Then, TLBO is proposed and applied to determine the optimum online path, where the objectives are to find the shortest path to reach the target destination, and to maximize the path smoothness, while avoiding the surrounding obstacles, and taking into account the vehicle dynamic and algebraic constraints. To check the performance of the proposed TLBO algorithm, it is compared in simulation to genetic algorithm (GA), particle swarm optimization (PSO), and a hybrid GA–PSO algorithm. Finally, real-time experiments based on robot operating system (ROS) implementation are conducted to validate the effectiveness of the proposed path planning algorithm.
The Relationship Between Timing, Speed, and Performance in Foreign Market Network Entry
Authors: Hilmersson Mikael , Schweizer Roger , Chetty Sylvie 
Published: June 2022

AbstractThe purpose of this article is to examine the performance consequences of an early and rapid foreign network entry process. We develop a theoretical model that uses theories on first mover advantage and capability development as mechanisms to explain the relationship between the timing and speed of entering the network and SME performance in the network. The theoretical model is tested with a dataset collected on-site at 198 SMEs. The results show that earlier network entry positively affects performance and that the speed of relationship development in the network entered has a curvilinear (inverted U-shaped) effect on SMEs’ performance. Although the extant literature recognizes that firm performance is affected by the degree of insidership reached in foreign business networks, little is known about the performance effects of the timing and the speed of entering the network. Our findings suggest that managers of exporting SMEs should move first into new networks to give themselves time to grow slowly in the foreign market network to mitigate diseconomies of time compression.
Normativität und Emotionalität. Eine anthropologisch-evolutionäre Perspektive auf die Soziogenese zweier miteinander verbundener Modi humaner Vergesellschaftung
Authors: Niedenzu Heinz-Jürgen 
Published: June 2022

ZusammenfassungIn diesem Beitrag geht es um die wechselseitige Verbindung von Normativität und Emotionalität in sozialen Prozessen. Einerseits wird die Wirksamkeit von Normen durch deren emotionale Inkorporierung mit abgesichert, andererseits werden affektiv-emotionale Befindlichkeiten normativ bearbeitet und sozial strukturiert. Im Beitrag wird diesem Wirkungszusammenhangs aus einer soziogenetischen Perspektive nachgegangen, d. h. die Frage nach dessen anthropologisch-evolutionstheoretischen Begründbarkeit gestellt. Im Anschluss an die Disziplinen übergreifende Programmatik der Philosophischen Anthropologie sowie der rekonstruktiv verfahrenden Erkenntnisstrategie der historisch-genetischen Theorie wird auf einer konzeptuellen Ebene diskutiert, wie sich Normativität und Emotionalität als zwei strukturell miteinander verbundene Modi der Weltzuwendung aus der Gattungsgeschichte heraus einsichtig machen lassen. Als Schlüsselmoment erweist sich dabei das evolutionär entstandene konstruktive Vermögen, welches es Menschen ermöglicht, ihre naturale Indeterminiertheit bzw. Weltoffenheit kulturell zu bewältigen. Im anlaufenden Vergesellschaftungsprozess wird die sich in Gang setzende Ausbildung von sinnbasierter Normativität von vorneherein mit der Umstrukturierung der organischen Affektbasis in Emotionalität verbunden.
Self-Reported Efficacy of Treatments in Cluster Headache: a Systematic Review of Survey Studies
Authors: Rusanen Sakari Santeri , De Suchetana , Schindler Emmanuelle Andree Danielle , Artto Ville Aleksi , Storvik Markus 
Published: June 2022

AbstractPurpose of ReviewThe use and efficacy of various substances in the treatment of CH have been studied in several retrospective surveys. The aim of the study is to systematically review published survey studies to evaluate the reported efficacies of both established and unconventional substances in abortive and prophylactic treatment of both episodic and chronic CH, specifically assessing the consistency of the results.Recent FindingsNo systematic review have been conducted of these studies previously. A systematic literature search with a set of search terms was conducted on PubMed. Retrospective surveys that quantified the self-reported efficacy of two or more CH treatments, published in English during 2000–2020, were included. Several key characteristics and results of the studies were extracted. A total of 994 articles were identified of which 9 were found to be eligible based on the selection criteria. In total, 5419 respondents were included. Oxygen and subcutaneous triptan injections were most reported as effective abortive treatments, while psilocybin and lysergic acid diethylamide were most commonly reported as effective prophylactic treatments. The reported efficacy of most substances was consistent across different studies, and there were marked differences in the reported efficacies of different substances. The reported order of efficacy is generally in agreement with clinical studies. The findings suggest that retrospective surveys can be used to obtain supporting information on the effects of various substances used in the treatment of CH and to form hypotheses about novel treatment methods. The consistently reported efficacy of psilocybin and LSD in prophylactic treatment indicates need for clinical studies.
Randomized phase II trial of FOLFIRI-panitumumab compared with FOLFIRI alone in patients with RAS wild-type circulating tumor DNA metastatic colorectal cancer beyond progression to first-line FOLFOX-panitumumab: the BEYOND study (GEMCAD 17-01)
Authors: Aparicio Jorge , Virgili Manrique Anna C. , Capdevila Jaume , Muñoz Boza Félix , Galván Patricia , Richart Paula , Oliveres Helena , Páez David , Hernando Jorge , Serrano Sara , Vera Ruth , Hernandez-Yagüe Xavier , Gallego Rafael Álvarez , Riesco-Martinez M. Carmen , García de Albeniz Xavier , Maurel Joan 
Published: June 2022

AbstractPurposePanitumumab plus FOLFOX (P-FOLFOX) is standard first-line treatment forRASwild-type (WT) metastatic colorectal cancer. The value of panitumumab rechallenge is currently unknown. We assessed addition of panitumumab to FOLFIRI (P-FOLFIRI) beyond progression to P-FOLFOX in patients with noRASmutations in liquid biopsy (LB).MethodsIn this randomized phase II trial, patients were assigned (3:2 ratio) to second-line P-FOLFIRI (arm A) or FOLFIRI alone (arm B). LB for circulating tumor DNA analysis was collected at study entry and at disease progression. Primary endpoint was 6-month progression-free survival. Two-stage Simon design required 85 patients to be included (EudraCT 2017-004519-38).ResultsBetween February 2019 and November 2020, 49 patients were screened (16 RAS mutations in LB detected) and 31 included (18 assigned to arm A and 13 to arm B). The study was prematurely closed due to inadequate recruitment. Serious adverse events were more frequent in arm A (44% vs. 23%). Overall response rate was 33% (arm A) vs. 7.7% (arm B). Six-month progression-free survival rate was 66.7% (arm A) and 38.5% (arm B). Median progression-free survival was 11.0 months (arm A) and 4.0 months (arm B) (hazard ratio, 0.58). At disease progression,RASorBRAFmutations in LB were found in 4/11 patients (36%) in arm A and 2/10 (20%) in arm B.ConclusionsThe BEYOND study suggests a meaningful benefit of P-FOLFIRI beyond progression to P-FOLFOX in metastatic colorectal cancer patients with WTRASstatus selected by LB. This strategy deserves further investigation.
Crosstalk between angiogenesis and immune regulation in the tumor microenvironment
Authors: Kim Hei Jung , Ji Young Rae , Lee You Mie 
Published: June 2022

AbstractCancer creates a complex tumor microenvironment (TME) composed of immune cells, stromal cells, blood vessels, and various other cellular and extracellular elements. It is essential for the development of anti-cancer combination therapies to understand and overcome this high heterogeneity and complexity as well as the dynamic interactions between them within the TME. Recent treatment strategies incorporating immune-checkpoint inhibitors and anti-angiogenic agents have brought many changes and advances in clinical cancer treatment. However, there are still challenges for immune suppressive tumors, which are characterized by a lack of T cell infiltration and treatment resistance. In this review, we will investigate the crosstalk between immunity and angiogenesis in the TME. In addition, we will look at strategies designed to enhance anti-cancer immunity, to convert “immune suppressive tumors” into “immune activating tumors,” and the mechanisms by which these strategies enhance effector immune cell infiltration.