bluuebunny/crossref_metadata_2025_split huggingface dataset:
Manajemen Asuhan Kebidanan Intranatal pada Ny. E dengan Preekalmpsia Berat
Authors: Alviana Alviana , Nurhayati Nurhayati , Rahmawati Rahmawati 
Published: February 2022

Data yang di dapatkan menurut sebuah studi World Health Organization (WHO) bahwa sekitar 810 wanita meninggal karena sebab yang dapat dicegah terkait dengan persalinan dan kehamilan. Komplikasi utama yang menyebabkan hampir 75% dari semua kematian ibu adalah tekanan darah tinggi selama kehamilan (Preeklampsia dan eklampsia, hipertensi gestasional) sekitar 14% yang menduduki posisi kedua setelah perdarahan dan disusul oleh komplikasi dari persalinan aborsi yang tidak aman.Di Indonesia preeklampsia berat dan eklampsia merupakan penyebab kematian ibu berkisar 1,5% sampai 25 %, preeklampsia yang termasuk dalam hipertensi dalam kehamilan menempati posisi kedua terbanyak setelah perdarahan yang menyebabkan kematian ibu. Tujuan disusunnya Laporan Tugas Akhir (LTA) ini adalah agar dapat melaksanakan Asuhan Kebidanan Intranatal Pada Ny”E” Dengan Preeklampsia Berat di RSUD Labuang Baji makassar dengan penerapan manajemen asuhan kebidanan sesuai wewenang bidan.
Preeklampsia merupakan komplikasi kehamilan dan persalinan yang ditandai dengan timbulnya hipertensi 160/110 mmHg atau lebih disertai proteinuria dan atau di sertai oedema pada kehamilan 20 minggu atau lebih. Gejala dari preeklampsia seperti hipertensi, oedema dan proteinuria sering tidak diperhatikan, sehingga tanpa disadari dalam waktu singkat dapat timbul menjadi preeklampsi berat, bahkan eklampsia.
Hasil Tinjauan kasus dengan tinjauan teori telah diuraikan dan ditemukan adanya kesenjangan antara tinjauan kasus dengan tinjauan teori serta upaya yang dilakukan berdasarkan lingkup tugas, wewenang, dan tanggung jawab bidan.
Setelah mengevaluasi hasil yang telah dilakukan pada Ny”E” dapat disimpulkan bahwa ibu preeklampsia berat.
Comprehensive Analysis of the Expression and Prognosis for ITGBs: Identification of ITGB5 as a Biomarker of Poor Prognosis and Correlated with Immune Infiltrates in Gastric Cancer
Authors: Liu Dongliang , Liu Shaojun , Fang Yu , Liu Liu , Hu Kongwang 
Published: February 2022

Background: Integrin β superfamily members (ITGBs) are documented to play important roles in various biological processes, and accumulating evidence suggests that ITGBs are associated with carcinogenic effects in several malignancies. Gastric cancer (GC) is a complicated and highly heterogeneous disease; however, the expression and prognostic values of eight ITGBs and potential mechanism in GC remain largely unclear.Methods: The expression and prognostic significance of ITGBs in GC were systematically analyzed through Gene Expression Profiling Interactive Analysis, Human Protein Atlas, Kaplan–Meier Plotter, and cBioPortal databases. Then, the mRNA transcription data and corresponding clinical data of GC were downloaded from the Gene Expression Omnibus database as a testing cohort, and differentially expressed and prognostic genes were identified. The correlation between ITGB5 expression and overall survival and various clinical parameters were found by using univariate/multivariable Cox regression and Kaplan–Meier survival analysis. Additionally, differential analysis of gene expression profiles in low- and high-ITGB5 expression groups and pathway enrichment analysis was performed. Finally, the correlation of ITGB5 expression with immune infiltrates in GC was clarified.Results: Compared with adjacent normal tissue, the results reveal that the mRNA levels of ITGB1-2 and ITGB4-8 are significantly higher in GC, and immunohistochemistry results show the consistency between RNA and protein expression levels. Cox regression and Kaplan–Meier survival analysis indicate that high ITGB5 expression contributes to a poor prognosis and could be an independent prognostic factor in GC patients. Besides this, gene functional enrichment analysis indicates that ITGB5 expression is significantly associated with extracellular matrix organization, cell-substrate adhesion, and ossification. The KEGG pathway analysis of ITGB5 shows a close association between ITGB5 and focal adhesion, ECM-receptor interaction, phagosome, and PI3K-Akt signaling pathway. Last, the infiltrating level of CD4+ T cells, macrophages, and dendritic cells are positively related to the expression of ITGB5, especially macrophages, and lower levels of macrophages predict a better prognosis in GC in our study.Conclusion: Our findings investigate that ITGB5 may function as a valid biomarker of prognosis, and high expression of ITGB5 predicts poor prognosis for patients with GC. Besides this, it might be a potential target of precision therapy against GC.
The Roles of Blood Lipid-Metabolism Genes in Immune Infiltration Could Promote the Development of IDD
Authors: Li Weihang , Ding Ziyi , Zhang Huan , Shi Quan , Wang Dong , Zhang Shilei , Xu Songjie , Gao Bo , Yan Ming 
Published: February 2022

Objectives: Intervertebral disc degeneration is a progressive and chronic disease, usually manifesting as low back pain. This study aimed to screen effective biomarkers for medical practice as well as figuring out immune infiltration situations between circulation and intervertebral discs.Methods: Gene expression profiles of GSE124272 was included for differentially analysis, WGCNA and immune infiltration analysis from GEO database, and other GSE series were used as validation datasets. A series of validation methods were conducted to verify the robustness of hub genes, such as principal component analysis, machine learning models, and expression verification. Lastly, nomogram was established for medical practice.Results: 10 genes were commonly screened via combination of DEGs, WGCNA analysis and lipid metabolism related genes. Furthermore, 3 hub gens CYP27A1, FAR2, CYP1B1 were chosen for subsequent analysis based on validation of different methods. GSEA analysis discovered that neutrophil extracellular traps formation and NOD-like receptor signaling pathway was activated during IDD. Immune infiltration analysis demonstrated that the imbalance of neutrophils and γδT cells were significantly correlated with IDD progression. Nomogram was established based on CYP27A1, FAR2, CYP1B1 and age, the calibration plot confirmed the stability of our model.Conclusion: CYP27A1, FAR2, CYP1B1 were considered as hub lipid metabolism related genes (LMRGs) in the development of IDD, which were regarded as candidate diagnostic biomarkers especially in circulation. The effects are worth expected in the early diagnosis of IDD through detecting these genes in blood.
Case Report: Hypergranular Platelets in Vaccine-Induced Thrombotic Thrombocytopenia After ChAdOx1 nCov-19 Vaccination
Authors: Comer Shane P. , Le Chevillier Ana , Szklanna Paulina B. , Kelliher Sarah , Saeed Khalid , Cullen Steven , Edebiri Osasere , O'Neill Tiina , Stephens Niamh , Weiss Luisa , Murphy Claire A. , Rajakumar Saraswathi , Tierney Alexandra , Hughes Conor , Lennon Áine , Moran Niamh , Maguire Patricia B. , Ní Áinle Fionnuala , Kevane Barry 
Published: February 2022

BackgroundVaccine-induced thrombotic thrombocytopenia (VITT) post SARS-CoV-2 vaccination is characterized by thrombocytopenia and severe thrombosis. Platelet function during patient recovery in the medium-/long-term has not been investigated fully. Here, we undertook a 3-month study, assessing the recovery of a VITT patient and assessing platelet morphology, granule content and dense-granule release at two distinct time points during recovery.Case PresentationA 61 year-old female was admitted to hospital 15 days post ChAdOx1 nCov-19 vaccination. Hematological parameters and peripheral blood smears were monitored over 3 months. Platelet morphology and granule populations were assessed using transmission electron microscopy (TEM) at two distinct time points during recovery, as was agonist-induced platelet dense-granule release. Upon admission, the patient had reduced platelet counts, increased D-dimer and high anti-PF4 antibodies with multiple sites of cerebral venous sinus thrombosis (CVST). Peripheral blood smears revealed the presence of large, hypergranular platelets. Following treatment, hematological parameters returned to normal ranges over the study period. Anti-PF4 antibodies remained persistently high up to 90 days post-admission. Two days after admission, VITT platelets contained more granules per-platelet when compared to day 72 and healthy platelets. Additionally, maximal ATP release (marker of dense-granule release) was increased on day 2 compared to day 72 and healthy control platelets.ConclusionThis study highlights a previously unreported observation of platelet hypergranularity in VITT which may contribute to the thrombotic risk associated with VITT. Optimal approaches to monitoring recovery from VITT over time remains to be determined but our findings may help inform therapeutic decisions relating to anticoagulation treatment in this novel pathology.
Early Permian Syn-Subduction Extension in the South Tianshan (NW China): Insights From A-Type Granitoids in the Southern Altaids
Authors: Abuduxun Nijiati , Xiao Wenjiao , Windley Brian F. , Huang Peng , Yang He , Gan Jingmin , Sang Miao , Liu Xijun 
Published: February 2022

A-type granite is an important geodynamic indicator because it requires a high melting temperature that is commonly driven by extensional events. Here we report geochronology, whole-rock geochemistry, and zircon Lu-Hf isotopes of newly identified A-type granitic rocks from the South Tianshan in the southern Altaids. Zircon LA-ICP-MS ages indicate that the granitoids were emplaced at ca. 298–272 Ma. Geochemically, they are metaluminous to slightly peraluminous (A/CNK = 0.95–1.10), and belong to the high-K calc-alkaline to shoshonitic series. They are characterized by relatively high zircon saturation temperatures (824–875°C), K2O + Na2O contents (7.31–9.36%), high field strength elements (HFSE; Zr + Nb + Ce + Y = 365–802 ppm), and Ga/Al ratios (2.8–4.2), which all point to an A-type affinity. In addition, they have slightly enriched Hf isotope compositions (εHf(t) = −10.9 to + 0.6), and corresponding Mesoproterozoic (1,272–1759 Ma) crustal model ages, suggesting they were probably generated by partial melting of mature crust that contained minor mantle-derived magmatic material. The granitoids have distinctive subduction-related trace element signatures, with deep Nb and Ta troughs, elevated large ion lithosphere elements (LILEs), and flat HFSEs patterns, very similar to arc-derived granites in the Lachlan accretionary orogen. Integration of these new sedimentological, structural and geochronological results with relevant published information provides a new data-archive, which indicates that neither the Tarim mantle plume nor post-collisional extension can explain the genesis of these A-type granitoids. Instead, we propose a new more pertinent and robust model according to which they formed due to high temperature gradient in a subduction-related extensional setting probably triggered by southward rollback of the South Tianshan oceanic lithosphere, which caused upwelling of asthenospheric mantle combined with an increased temperature that led to large-scale crustal melting. This process gave rise to a broad magmatic arc in the southern active margin of the Yili-Central Tianshan. Our new data shed light on the retreating accretionary orogenesis of the southern Altaids in the Permian.
Changes in the Surface Elevation of the Laohugou Glacier No. 12 in Western Qilian Mountains
Authors: Liu Yushuo , Qin Dahe , Li Yanzhao , Qin Xiang , Li Zhenlin , Wang Jing , Jin Zizhen , Wang Lihui 
Published: February 2022

As the largest valley glacier in the Qilian Mountains, the Laohugou glacier No. 12 (LHG12) has shrunk significantly since 1957. In this study, two topographic maps and a WorldView-2 satellite stereopair image data were used to assess the volume and cumulative mass balance of LHG12 located at the western Qilian Mountains during 1957–2015. During the study period, the LHG12 exhibited changes in two processes: slightly ablation and stability in a brief period during 1957–1989 and strong melting and accelerated ablation during 1989–2015. During 1957–2015, the volume of LHG12 decreased by 0.38 km3, the average thickness decreased by 17.23 m, the cumulative mass balance (MB) was −14.69 ± 3.00 m w. e., and ablation was found glacier-wide. By comparing the previous MB simulation and digital elevation model (DEM) differencing results, it was found that the MB simulation results underestimated the strong melting trend of LHG12 since the 1990s. Temperature rose, especially in autumn and winter, and could cause the ice temperature of LHG12 to increase, and LHG12 may become more sensitive to climate change.
Experimental and Numerical Study on Thermal Performance of a Novel Direct Expansion Air-Source Heat Pump Heating System
Authors: Wang Haobo , Cheng Yuanda , Wang Dawei , Peng Weijin , Ai Zejian , Jia Jie , Duan Pengfei , Yang Jinming 
Published: February 2022

In this paper, a novel direct expansion air-source heat pump heating system with a gravity-driven radiator as a heating terminal was developed, for the purpose of reducing the energy consumption and improving the thermal comfort of indoor personnel. The thermal response speed of the system at the start-up stage was experimentally studied firstly. The results showed that the thermal response speed of the developed system was slightly lower than that of a traditional air-source heat pump heating system with a convective heating terminal, but largely higher than that of an air-source heat pump floor radiant heating system. When the outdoor air temperature fluctuated between −5.2℃ and −1.0℃, the average rising ratios of indoor air temperature at the start-up stage for the developed heating system and the traditional system are 10.8℃/h and 13.8℃/h respectively. On this basis, an optimization study was then conducted to further improve the thermal performance of the developed heating system. The numerical results indicated that integrating a cross-flow fan to the gravity-driven radiator is able to promote the thermal performance of the heating system obviously at the start-up stage, because the convection heat transfer of the radiator was highly enhanced. Among the simulation conditions, the time required for the air temperature at head height to reach the designed temperature is shortest when the flow angle of the fan is 0° with an air speed of 0.6 m/s. while setting the flow angle of the fan to −45° with an air speed of 0.4 m/s is recommended to achieve the most thermal comfort for the whole body.
Metabolomic Profiling Reveals That 5-Hydroxylysine and 1-Methylnicotinamide Are Metabolic Indicators of Keloid Severity
Authors: Shan Mengjie , Liu Hao , Hao Yan , Song Kexin , Meng Tian , Feng Cheng , Wang Youbin , Huang Yongsheng 
Published: February 2022

Background: Keloid is a skin fibroproliferative disease with unknown pathogenesis. Metabolomics provides a new perspective for revealing biomarkers related to metabolites and their metabolic mechanisms.Method: Metabolomics and transcriptomics were used for data analysis. Quality control of the data was performed to standardize the data. Principal component analysis (PCA), PLS-DA, OPLS-DA, univariate analysis, CIBERSORT, neural network model, and machine learning correlation analysis were used to calculate differential metabolites. The molecular mechanisms of characteristic metabolites and differentially expressed genes were identified through enrichment analysis and topological analysis.Result: Compared with normal tissue, lipids have a tendency to decrease in keloids, while peptides have a tendency to increase in keloids. Significantly different metabolites between the two groups were identified by random forest analysis, including 1-methylnicotinamide, 4-hydroxyproline, 5-hydroxylysine, and l-prolinamide. The metabolic pathways which play important roles in the pathogenesis of keloids included arachidonic acid metabolism and d-arginine and d-ornithine metabolism. Metabolomic profiling reveals that 5-hydroxylysine and 1-methylnicotinamide are metabolic indicators of keloid severity. The high-risk early warning index for 5-hydroxylysine is 4 × 108-6.3×108 (p = 0.0008), and the high-risk predictive index for 1-methylnicotinamide is 0.95 × 107-1.6×107 (p = 0.0022).Conclusion: This study was the first to reveal the metabolome profile and transcriptome of keloids. Differential metabolites and metabolic pathways were calculated by machine learning. Metabolomic profiling reveals that 5-hydroxylysine and 1-methylnicotinamide may be metabolic indicators of keloid severity.
Mass Cytometry Study on Hepatic Fibrosis and Its Drug-Induced Recovery Using Mouse Peripheral Blood Mononuclear Cells
Authors: Bae Jiwon , Kim Ji Eun , Perumalsamy Haribalan , Park Sehee , Kim Yun , Jun Dae Won , Yoon Tae Hyun 
Published: February 2022

The number of patients with liver diseases has increased significantly with the progress of global industrialization. Hepatic fibrosis, one of the most common liver diseases diagnosed in many developed countries, occurs in response to chronic liver injury and is primarily driven by the development of inflammation. Earlier immunological studies have been focused on the importance of the innate immune response in the pathophysiology of steatohepatitis and fibrosis, but recently, it has also been reported that adaptive immunity, particularly B cells, plays an essential role in hepatic inflammation and fibrosis. However, despite recent data showing the importance of adaptive immunity, relatively little is known about the role of B cells in the pathogenesis of steatohepatitis fibrosis. In this study, a single-cell-based, high-dimensional mass cytometric investigation of the peripheral blood mononuclear cells collected from mice belonging to three groups [normal chow (NC), thioacetamide (TAA), and 11beta-HSD inhibitor drug] was conducted to further understand the pathogenesis of liver fibrosis through reliable noninvasive biomarkers. Firstly, major immune cell types and their population changes were qualitatively analyzed using UMAP dimensionality reduction and two-dimensional visualization technique combined with a conventional manual gating strategy. The population of B cells displayed a twofold increase in the TAA group compared to that in the NC group, which was recovered slightly after treatment with the 11beta-HSD inhibitor drug. In contrast, the populations of NK cells, effector CD4+T cells, and memory CD8+T cells were significantly reduced in the TAA group compared with those in the NC group. Further identification and quantification of the major immune cell types and their subsets were conducted based on automated clustering approaches [PhenoGraph (PG) and FlowSOM]. The B-cell subset corresponding to PhenoGraph cluster PG#2 (CD62LhighCD44highLy6chighB cells) and PG#3 (CD62LhighCD44highLy6clowB cell) appears to play a major role in both the development of hepatic fibrosis and recoveryviatreatment, whereas PG#1 (CD62LlowCD44highLy6clowB cell) seems to play a dominant role in the development of hepatic fibrosis. These findings provide insights into the roles of cellular subsets of B cells during the progression of, and recovery from, hepatic fibrosis.
Catatonic Schizophrenia Associated With Cerebrospinal GAD65 Autoantibodies: Case Report and Literature Review
Authors: Hansen Niels , Bartels Claudia , Teegen Bianca , Wiltfang Jens , Malchow Berend 
Published: February 2022

BackgroundGAD65 autoimmunity is reported to be associated with schizophrenia and bipolar disorder. However, there has been no evidence that glutamic acid decarboxylase 65 (GAD65) autoantibodies in cerebrospinal fluid (CSF) are associated with akinetic catatonia in schizophrenia patients.MethodsWe report the case of a 28-year-old man who underwent diagnostics including brain MRI, neuropsychological testing, and electroencephalography (EEG) as well as a tumor search via CT of the abdomen and thorax, as well as colonoscopy and gastroscopy. For clinical characterization, his patient files were retrospectively examined.ResultsOur patient presented catatonia that responded somewhat to benzodiazepines in combination with previously taken antipsychotics such as risperidone for prediagnosed paranoid schizophrenia. Diagnostics revealed GAD65 autoantibodies in his serum and CSF. MRI revealed no brain lesion, and the tumor search had no malignancy. We diagnosed catatonic schizophrenia. Furthermore, as he had not fully recovered, he was given immunotherapy entailing two cycles of intravenous immunoglobulins. Subsequent neuropsychological testing due to subjective cognitive complaints after immunotherapy revealed no objective cognitive deficits.ConclusionsWe present the novel finding of an association between GAD65 autoantibodies in the serum and CSF with catatonia in a patient suffering from prediagnosed chronic schizophrenia. Due to the presence of CSF GAD65 antibodies and the catatonia factor in prediagnosed schizophrenia, we suspect that his catatonia has an autoimmune origin. Immunotherapy stabilized the catatonia that had initially responded to lorazepam treatment. Further research should be done to characterize patients’ responses to immunotherapy and standard treatment in a large cohort of patients with GAD65 antibody-associated catatonia and schizophrenia.
Interleukin-10 Is a Promising Marker for Immune-Related Adverse Events in Patients With Non-Small Cell Lung Cancer Receiving Immunotherapy
Authors: Wang Haowei , Zhou Fei , Zhao Chao , Cheng Lei , Zhou Caicun , Qiao Meng , Li Xuefei , Chen Xiaoxia 
Published: February 2022

BackgroundImmune checkpoint inhibitors (ICIs) brought about a major paradigm shift in non-small cell lung cancer (NSCLC) treatment. However, the use of ICIs is related to an unforeseeable pattern of immune-related adverse events (irAEs). Hence, more precise biomarkers are needed to predict the incidence of irAEs to prevent overtreatment of ICIs and decrease occurrences of irAEs. This study was designed to identify capable clinical features and plasma inflammatory factors for predicting irAEs.MethodsA total of 67 patients who received ICI monotherapy or ICI-based combination therapy were retrospectively identified. Clinical characteristics and plasma inflammatory cytokines were collected and analyzed to screen potential biological markers associated with irAEs. The chi-square test, Fisher’s test, and the Mann–Whitney U test were performed for the primary analysis. The optimal cutoff value was determined by a receiver operating characteristic (ROC) curve. Univariate and multivariate logistic regression models were used to identify risk factors of irAEs. Univariate and multivariate Cox proportional hazards were also performed.ResultsOut of 67 patients, 40 (59.7%) experienced irAEs, and 7 (10.4%) experienced severe adverse events (grade ≥ 3). Among these analyzed immune profile biomarkers, only interleukin−10 (IL-10) was related to the risk of irAEs. A high baseline IL−10 plasma level (odds ratio (OR) = 5.318, 95% CI 1.174–24.081, p = 0.030) was found to be a tremendous and independent risk factor for the development of irAEs. Also, for the dynamic analysis, upregulation of IL-10 after one cycle of ICI treatment was positively related to the occurrence of irAEs (OR = 5.712, 95% CI 1.088–29.993, p = 0.039). When pneumonitis, the most common irAEs, was analyzed, only baseline high-expression IL-10 was accompanied with the incidence of pneumonitis (OR = 9.969, 95% CI 1.144–86.843, p = 0.037).ConclusionBaseline and dynamic IL-10 plasma levels are tremendously and independently related to higher risk in the development of irAEs and could be utilized for medical practice to monitor adverse events in patients with ICI treatment.