bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of key biomarkers and immune infiltration in the peripheral blood of osteoarthritis by bioinformatics analysis
Authors: Ma Tiancong , Zhao Guanglei , Huang Xin , Lyu Jinyang , Huang Gangyong , Wang Siqun , Wei Yibing , Xia Jun 
Published: May 2022

Abstract
Background Osteoarthritis (OA) is a common chronic arthritis disease characterized by cartilage degeneration, sub-cartilage bone hyperplasia, osteophyte formation and joint space stenosis. Traditionally, OA treatment consists of pain management with joint replacement for end-stage disease. Patients diagnosed with OA have developed irreversible organic changes, so the focus should be shifted to prevention, early diagnosis and intervention of OA.Methods The differentially expressed genes (DEGs) were identified from the gene expression profile GSE48556 from the GEO database, and functional enrichment analyses were performed. Boruta algorithm and the least absolute shrinkage and selection operator (LASSO) analysis were used to select hub genes, then we used the LightGBM to evaluate the accuracy of hub genes prediction for OA. The CIBERSORT algorithm was used to analyze the immune infiltration of peripheral blood mononuclear cells (PBMCs) between OA and normal controls.Results A total of 35 DEGs including 29 downregulated genes and 6 upregulated genes were mainly involved in ﻿response to tumor necrosis factor (TNF) and TNF-mediated signaling pathway through GO analysis and were mainly enriched in cAMP signaling pathway, NF-κB signaling pathway through KEGG. The LightGBM prediction of the hub gene model showed that area under curve (AUC) of ROC curve and PR curve reached 0.988 and 0.996 respectively in verification set, indicating that hub genes have a high diagnostic accuracy for OA. Gene ADRB2 was the only up-regulated gene in OA group. H3F3B showed the largest AUC which means a high diagnostic power of OA. From the immune infiltration analysis, the content of CD8+ T cells and resting mast cells showed significant differences between the two groups and hub genes were closely related to immune cells.Conclusion The hub genes in PBMCs between OA and normal controls may potentially serve as biomarkers in both prevention and diagnosis of OA and indicate for the exploration of the mechanism of OA.
Cost and time reduction of industrial mold design and manufacturing by implementing additive manufacturing for premature neonatal prong
Authors: mirnia kayvan , Heidarzadeh Mohammad , Maghsoudi Amid , Pourashouri Zahra ,   Abbas Abaei Kashan5
Published: May 2022

Abstract

Introduction: For a long time, molding was one of the most important methods of producing metal, ceramic, and polymer materials. The two essential factors in this method were always cost and time. Nowadays, with a computer and additive manufacturing technology, you can design in 3D. This article describes how to reduce the time and cost to get the final product using 3D-Printers. The design and production of molds for premature neonatal respiratory aid, called "Prong," were applied based on neonatologists' considerations.Method: This study is performed in Alzahra hospital in Tabriz University on fifteen very low birth neonates from September 2017 till September 2019. In the first section, we described dental plaster material for molding. To use this material, select the printing material to control the parameters, including melting temperature and printer speed, so that the final sample results with acceptable quality. If the final three-dimensional design is suitable, various objects can be printed using CAD (Computer-Aided Design) software.Results: By using additive manufacturing technology, we achieved a new design with a little creativity and trial and error, and we also solved bubble problems at a low cost. So, we designed some cavities in the new mold to ensure that silicon takes up all of the mold space and provides a way to exit the bubble in the silicon.Conclusion: To achieve an optimal and final design for prong mold. Using 3D printers helps us reduce production costs and time and optimize the design. Finally, the CNC machine produced the ultimate mold made of aluminum. We tested the final product in Al-Zahra Hospital in Tabriz in Iran, and the results were satisfactory, and there were no reports of necrosis on the babies' noses.
CosSinO: A Trigonometric Rule-based Deterministic Gaming Algorithm in a Multi-player Environment
Authors: Sahu Shiv kumar , Panigrahi Niranjan 
Published: May 2022

Abstract
Game Playing has been an attractive field of research in Artificial Intelligence (AI) domain. Different category of games are evolved since its inspection which are based on rules and heuristics and investigated under AI scope. In this article, we have presented a trigonometric rule-based, multi-player board game, named as CosSinO, with its graphical layout, playing rules and the deterministic algorithm. The auxiliary data structure needed for the algorithm is a Trigonometric Function Mapper (TFM) which is realized as a look-up table. The output of the game is stored in a score table which is basically an n x m matrix where n is the number of players and m is the number of mutually agreed trials among the players. The time and space complexity of the algorithm is proven to be polynomial and is O (nm). The game in its physical form has been tested exhaustively by exposing it to different group of players and it is proven to be an exciting game among trigonometry lovers
Evaluating the Knowledge about Older Patients Quiz and the Kogan's Attitudes towards Old People Scale for Nursing Students’ Gerontological Learning Experiences in Ghana.
Authors: Abudu-Birresborn Diana , Brennenstuhl Sarah , Puts Martine , McCleary Lynn , Yakong Vida , Chu Charlene H , Cranley Lisa 
Published: May 2022

Abstract
Background: Understanding nursing students’ knowledge about and attitudes towards older adults’ using a context-specific survey instrument may help to identify, and design effective learning and teaching materials to improve the care for older adults. The study aimed to evaluate the items on the Knowledge about Older Patients Quiz and the Kogan's Attitudes towards Old People Scale suitable for the African context. Methods: A cross-sectional study was conducted using second- and third-year nursing students from two public Nursing Training Institutions in Ghana. Data were collected from December 2019 - March 2020 using the Knowledge about Older Patients Quiz and the Kogan’s Attitudes Towards Old People Scale. Item response theory was employed to evaluate the difficulty level and discrimination indices of the Knowledge about Older Patients Quiz. Corrected item-to-total correlation analysis was conducted for the Kogan's Attitudes towards Old People scale. The internal consistency for both scales was examined.Results: Of the 170 participants, 169 returned completed surveys. The mean age of participants was 21years (SD = 3.7), and (54%) were female. Of the 30-items of the Knowledge about Older Patients Quiz, seven items were very difficult for most of the students to choose the correct response, and one was easy, as most of the students chose the correct response. Although, 22 items demonstrated appropriate difficulty level, discrimination indices, were used to select the final 15- items that discriminated moderately between upper and lower 25% performing students. The Kuder-Richardson-20 reliability was 0.30. Considering the Kogan's Attitudes towards Old People scale, 10-items were removed following negative and low corrected item-to-total correlation and a high Alpha coefficient if items were deleted. The final 22-items had a Cronbach alpha coefficient of 0.65.Conclusion: Evaluation of the scales demonstrated essential content validity and moderate internal consistency for the context of our study. Further research should focus on ongoing context specific refinement of the survey instruments to measure nursing students’ knowledge about and attitudes towards the care of older adults in the African context.
Natural history and cycle threshold values analysis of the first factory outbreak of the delta variant of COVID-19 in Xiamen, China
Authors: Deng Bin , Liu Weikang , Guo Zhinan , Yang Tianlong , Huang Jiefeng , Abudunaibi Buasiyamu , Zhang Yidun , Ouyang Xue , Wang Demeng , Su Chenghao , Chen Tianmu 
Published: May 2022

Abstract
Objective: This study elaborated the natural history parameters of Delta variant, explored the differences in detection cycle thresholds (Ct) among cases.Method: Natural history parameters were calculated based on the different onset times and exposure times of the cases. Intergenerational relationships between generations of cases were calculated. Differences in Ct values of cases by gender, age, and mode of detection were analyzed statistically to assess the detoxification capacity of cases.Result: The median incubation period was 4 days; the detection time for cases decreased from 25 to 7 hours as the outbreak continued. The mean intergenerational, intergenerational infectiousness and intergenerational symptomatology were 3.6, 1.67 and 1.7 days. Among the Ct values, we found little differences in testing across companies, but there were some differences in the gender of detected genes. The Ct values continuous to decreased with age, but increased when the age was greater than 60.Conclusion: This epidemic was caused by the aggregation of factories. The effective reproduction number and real-time reproduction number were calculated using symptom intergenerational relationship (SI). And the analysis of Ct values can improve the positive detection rate and improve prevention and control measures.
Deviating HER2 test results in gastric cancer – analysis from the prospective multicenter VARIANZ study
Authors: Kolbe Katharina , Haffner Ivonne , Schierle Katrin , Maier Dieter , Geier Birgitta , Luber Birgit , Bläker Hendrik , Wittekind Christian , Lordick Florian 
Published: May 2022

Abstract
Purpose
The prospective multicenter VARIANZ study aimed to identify resistance biomarkers for HER2-targeted treatment in advanced gastric and esophago-gastric junction cancer (GC, EGJC). HER2 test deviations were found in 90 (22.3%) of 404 cases (central versus local testing) and were associated with a negative impact on survival for trastuzumab treated patients. Here, we investigated methodological and biological variables that may promote deviating HER2 test results.
Methods
We analyzed HER2 testing procedures and participation in quality assurance programs of 105 participating local pathology laboratories. Furthermore, tumor localization and histological subtypes were compared between patients with centrally confirmed (central HER2+ /local HER2+, n = 68) and unconfirmed HER2 status (central HER2- /local HER2+, n = 68).
Results
Central confirmation of the local HER2 immunohistochemical (IHC) scores were seen for the majority of locally HER2- IHC 0/1 (172/178; 96.6%), but less frequently for locally IHC3+ (57/124; 46.0%) cases. The deviation rate was not associated with the IHC antibody platform used in the local pathology institute neither with participation in quality-assuring round robin tests. Regarding tumor characteristics, deviating test results were more frequently found in GC vs. EGJC (69.1% vs. 39.7%; p = 0.001) and in Laurén diffuse vs. intestinal subtype (23.5% vs. 5.9%, p = 0.004).
Conclusion
Tumor localization and histological subtype have an impact on HER2 test deviation rates. Assessment of HER2 remains challenging and has an impact on survival outcomes of patients treated with trastuzumab for advanced GC and EGJC.
The Prevalence of Gingivitis and Related Risk Factors in Schoolchildren Aged 6–12 Years Old
Authors: Liu Xiaoyu , Xu Jianhui , Li Siwei , Wang Xueqin , Liu Jin , Li Xin 
Published: May 2022

Abstract
Background: Although epidemiological studies have consistently shown that gingivitis is a common disease, there is considerable variation between prevalence rates. This study aimed to understand the epidemiological characteristics of gingivitis, including prevalence, severity, intraoral distribution, and associated risk factors of each classification of gingivitis, in children aged 6–12 years in Jinzhou, China.Methods: A multistage, whole-group, randomized sample of 2,880 children aged 6–12 years in Jinzhou City, China, was selected and clinically examined. Each selected child completed a questionnaire on sociodemographic factors and oral health behaviors associated with gingivitis in collaboration with the investigator and teacher. Bleeding gums were recorded using the gingival index, and bleeding gum was defined as gingivitis when the number of bleeding teeth was greater than or equal to 10% of the number of teeth examined. Gingivitis was further categorized into localized gingivitis (30%≥number of teeth positive for gingival bleeding≥10%) and generalized gingivitis (number of positive for gingival bleeding >30%).Results: The prevalence of gingivitis in children aged 6–12 years in Jinzhou was 28.58%, including 701 cases of localized gingivitis (24.3%) and 122 cases of generalized gingivitis (4.2%). There were 429 cases (28.3%) of gingivitis in males and 394 cases (28.9%) in females, with no statistically significant difference in prevalence between males and females (P>0.05). Chi-square tests and binary logistic regression analysis showed that aging, tusk, plaque, and dental crowding were significantly associated with a high prevalence of gingivitis.Conclusions: Our study showed that tartar, heavy plaque, poor oral health behavior, and oral health awareness are associated with the prevalence of gingivitis and maintaining children's oral health requires professional guidance and regular preventive care.
Effect of magnesium sulfate on retinal dopaminergic neurons in rats with 6-OHDA-induced Parkinson’s disease
Authors: Huang Leyi , Lin Renxi , Zhang Chunying , Lin Ling 
Published: May 2022

Abstract
Parkinson's disease (PD) is one of the most common neurodegenerative diseases. The main pathological features of PD are the degeneration and death of dopamine neurons in the substantia nigra-striatal pathway and the accumulation of Lewy bodies in neurons. In addition to the well-known hallmark symptoms such as resting tremor and muscle rigidity, Parkinson's patients also experience non-motor symptoms such as visual disturbances, hyposmia, and sleep disturbances. At the same time, visual disturbances appear earlier than motor symptoms. The visual dysfunction in early-stage of Parkinson's disease indicates the occurrence and development of the disease. The visual dysfunction in Parkinson's disease is due to the damage of retinal dopamine neurons. This study found that magnesium sulfate supplementation can effectively delay the death of retinal dopamine neurons in 6-OHDA induced rat model of PD and improve the motor symptoms of rats with anxiety-like behavior. Therefore, magnesium sulfate may provide a new option for the early diagnosis and treatment of Parkinson's disease.
Cue-induced temporal attention affects contrast response function by response gain
Authors: Jing Chengxu , Zeng Yongjie , Li Wenxia , Chen Yao , Huang Dan 
Published: May 2022

Abstract
Orienting attention to a specific point in time has been shown to improve the contrast sensitivity at the attended time point and impair it earlier or later. This phenomenon could be explained by temporal attention increasing the effective contrast of target presented at the attended time point which leads to changes in contrast psychometric function by contrast gain. Another explanation is that temporal attention proportionally amplifies the amplitude of (behavioral or neural) response to contrast, resulting in alterations in contrast psychometric function by response gain. To explore the underlying mechanism, we adopted a temporal cueing orientation discrimination task using audio pre-cues composed of different frequency components to induce different attentional bias in time domain and targets of various contrast intensities to measure contrast psychometric functions. Obtained psychometric functions for contrast sensitivity were fitted for different conditions with discrepant attention states in time. We found that temporal attention manipulated by cue affected contrast psychometric function by response gain, indicating that multiplying contrast response of visual target occurring at the selected point in time by a fixed factor is a crucial way for temporal attention to modulate perceptual processing.
Panitumumab-associated stomatitis in metastatic colorectal cancer patients: clinical characterization and pathogenic considerations
Authors: Gomes-Silva Wagner , Vechiato-Filho Aljomar José , Luiz Ana Cláudia , Guollo André , de Oliveira Maria Cecília Querido , Gomes-Neves Maurício , Caparelli Fernanda Cunha , Brandão Thais Bianca 
Published: May 2022

Abstract
Purpose: The aim of this study was to report the clinical characterization and management of oral lesions affecting metastatic colorectal cancer (mCRC) patients undergoing panitumumab-containing regimens. Methods: Electronic medical records of mCRC patients referred to treat mouth sores during the treatment with the EGFR monoclonal antibody – panitumumab – were retrospectively reviewed at two centers. Patients’ characterization, clinical profile of oral lesions and management outcomes were documented. Additionally, modifications or discontinuation of the antineoplastic treatment as well as the occurrence of other adverse events (AEs) were analyzed. Results: A total of 8 patients were included. The oral lesions appeared in a mean time of 9 days (range 7-11 days) following the first cycles. Mean reported pain scores was 6 (range 1-9), causing feeding discomfort. The oral lesions showed a marked aphthous-like appearance in all cases and involved non-keratinized mucosa more likely. Painful atrophic tongue lesions (25%) and angular fissures (37.5%) were also observed. Two patients (25%) required dose reductions or interruptions of the antineoplastic treatment and 1 patient (12,5%) needed discontinuation due to panitumumab-associated stomatitis. Dermatologic AEs were the most prevalent. Clinical improvement was obtained in all patients following management with topical corticosteroid therapy and/or photobiomodulation. Conclusion: Panitumumab-containing regimes were associated with a particular pattern of oral lesions consistent with stomatitis. Although the lesions responded satisfactorily to corticosteroids and photobiomodulation, modifications or discontinuation of the antineoplastic treatment may eventually occur. Anti-EGFR effects of panitumumab on oral mucosa cells homeostasis seem to play a central role in the pathogenesis of this event.
Weighted composition, Volterra and Integral operators on Hardy Zygmund-type spaces
Authors: Hassanlou Mostafa , Abbasi Ebrahim 
Published: May 2022

Abstract
In this paper, we investigate weighted composition, Volterra and Integral operators on second derivative Hardy spaces. Some equivalent conditionsfor boundedness of the operators will be given using the boundedness on the Hardy spaces. Also we give a criteria forcompactness of weighted composition operators.
Taper off treatment can improve executive functions and regulate expression of BDNF and 5HTTPLR genes in opium abusers after six months period
Authors: Dezhakam Ani , Dezhakam Hossein , Haghighatfard Arvin 
Published: May 2022

Abstract
Opium is the dried latex obtained from the opium poppy. Opium addiction is the most prevalent addiction in Iranian society. During the last two decades "congress 60" a nongovernmental organization, has been performed a taper off treatment of opium associated with a package of psychological treatment group classes. While the effectiveness of taper off method in opium addiction has been confirmed, molecular mechanisms of this treatment in addicts are not clarified. BDNF gene is a brain-derived neurotrophic factor which is related to several molecular mechanisms of the brain including memory. 5-HTTPLR is a transporter of serotonin that is related to the decision-making process. In present study mRNA level of BDNF and 5-HTTPLR genes in peripheral blood of 20 non-psychiatric persons and 79 opium addicted individuals before and after six months period of taper off treatment was examined by using Real-Time PCR and confirmed by protein level analysis using Western blotting. In addition, executive functions including memory and decision making were analyzed in all participants. Results showed significant down expression of BDNF and 5-HTTPLR in addict persons vs. non psychiatric persons. Also significantly increase detected in BDNF (p < 0.01) and 5-HTTPLR (p < 0.01) expression level in addicts after six months of therapy period. Similar results with gene expression results revealed in protein level analysis. Also, significant improvements in memory and decision making were revealed in addicts after therapy and these improvements were correlated with the expression level of BDNF and 5-HTTPLR. Findings revealed the effect of opium abuse and taper off treatment on the expression of BDNF and 5-HTTPLR. In addition, the association of BDNF mRNA level with psychological states of addict's individuals detected. Results of the present study may help to a better understanding of molecular and neuropsychological mechanisms of opium addiction and taper off treatment. Also, present BDNF and 5-HTTPLR as potential markers for screening the effectiveness of different kinds of addiction treatment.
Protective effect of Allium Scorodoprasum L. ethanolic extract in cyclophosphamide- induced hepatotoxicity model in rats Short Title: Allium Scorodoprasum L. extract can be protective in hepatotoxicity
Authors: GUNGOR Hüseyin , EKICI Mehmet , KARATAS Ozhan , DiK Burak 
Published: May 2022

Abstract
Purpose: Cyclophosphamide(CP) is a chemotherapeutic agent and immunosuppressant drug however it damages the liver. The aim of this study is to investigate the protective effect of ethanolic extract of Allium scorodoprasum(ASE) on CP-induced liver injury. Methods: A total of 28 Wistar albino rats were randomly divided into four groups (n=7/group): Healthy, CP(200 mg/kg), CP(200 mg/kg)+ASE(100 mg/kg), CP(200 mg/kg)+ASE(200mg/kg). ASE was administered for 14 days, and the rats were euthanized 24 hours after CP administration. Results: CP Treatment leads to an increase in serum levels ALT, AST, LDH, total cholesterol, triglycerides, LDL, VLDL and liver levels MDA, TNF, IL-1β and IL-6, while HDL levels decrease, statistically. Treatment with CP caused liver necrosis and postnecrotic cell infiltration; however, the pathologic changes were prevented by ASE. Immunohistochemically, 8-OhDG, 4-HNE, and diTYR levels increased in the treatment with CP, whereas they decreased in the groups treated with ASE. All these changes were dose dependent in the ASE-treated groups. Conclusion: The treatment with CP caused liver damage due to oxidative stress and inflammation. ASE regulated the damage at high doses because it has potent antioxidant and anti-inflammatory ingredients. In future studies, it may be more beneficial to use ASE in higher doses or for a longer period of time.