bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis
Authors: Qi Huiying , Cao Huijuan , Zhao Yajie , Cao Yaqin , Jin Qide , Wang Yeping , Zhang Kun , Deng Daogui 
Published: November 2022

Abstract
Background
Molting is an important physiological process in the growth and development of arthropoda, which is mainly regulated by juvenile hormone and ecdysone. CYP302A1 is a key enzyme which plays a critical role in the synthesis of ecdysone in insects, but it has not been identified in cladocera.
Results
The CYP302Al gene of D. sinensis was cloned and its function was analyzed in this paper. The CYP302Al gene of D. sinensis was 5926 bp in full-length, with an open reading frame (ORF) of 1596 bp that encoded 531 amino acids, a molecular weight of 60.82 kDa and an isoelectric point of 9.29. The amino acid sequence analysis revealed that there were five characteristic conserved regions of cytochrome P450 family (namely helix-C, helix-K, helix-I, PERF and heme-binding). In dsRNA mediated experiment, the expression level of CYP302A1 gene decreased significantly (knock-down of 56.22%) in the 5% Escherichia coli concentration treatment. In addition, the expression levels of EcR and USP and HR3 genes in the downstream decreased also significantly, whereas that of FTZ-f1 gene increased significantly. In the 5% E. coli concentration treatment, the molting time at first pregnancy of D. sinensis prolonged, and the development of embryos in the incubation capsule appeared abnormal or disintegrated. The whole-mount in situ hybridization showed that the CYP302A1 gene of D. sinensis had six expression sites before RNA interference (RNAi), which located in the first antennal ganglion, ovary, gastric coeca, olfactory hair, thoracic limb and tail spine. However, the expression signal of the CYP302A1 gene of D. sinensis disappeared in the first antennal ganglion and obviously attenuated in the ovary after RNAi.
Conclusions
In conclusion, the CYP302A1 gene played an important role in the ecdysone synthesis pathway of D. sinensis, and the knock-down of the gene affected the molting and reproduction of D. sinensis.
Evaluating spatial and temporal patterns of tick encounters using community science data submitted through a smartphone application
Authors: Kache Pallavi A , Bron Gebbiena M. , Zapata-Ramirez Sandra , Tsao Jean I. , Bartholomay Lyric C. , Paskewitz Susan M. , Diuk-Wasser Maria A. , Fernandez Maria del Pilar 
Published: November 2022

Abstract

Research initiatives that engage the public (i.e., community science) increasingly provide insights into tick exposures in the United States. However, these data have limitations, particularly with respect to reported travel history and tick identification. Here, we assessed whether The Tick App provides reliable and novel insights into tick exposures across three domains—travel history and habitat exposure, broad spatial and temporal patterns of species-specific encounters, and tick identification. During 2019–2021, we received 11,424 tick encounter submissions from across the United States, with nearly all generated in the Midwest and Northeast regions. Encounters were predominantly with human hosts (71%); although one-fourth of ticks were found on animals. Half (51%) of the reported encounters resulted from peri-domestic exposures, while 37% were recreational exposures. Using phone-based location services, we detected differences in travel history outside of the users’ county of residence along an urbanicity gradient. Approximately 75% of users from large metropolitan and rural counties had travel out-of-county in the four days preceding tick detection, whereas an estimated 50–60% of users from suburban or smaller metropolitan areas did. Furthermore, we generated tick encounter maps for Dermacentor variabilis and Ixodes scapularis that accounted for travel history to the extent possible—overall mirroring previously published species distributions, while revealing 45 counties with new reports of D. variabilis exposures. Finally, using photo submissions, prompts of tick coloration and size engaged and guided users towards species and life stage classification moderately well, with 60% of users correctly identifying D. variabilis adults and 46% correctly identifying I. scapularis adults. Together, these results indicate the importance of bolstering the use of mobile applications to engage community scientists and complement other methods of active and passive tick surveillance.
Endometriosis, infertility and occupational life: women's plea for recognition
Authors: Gremillet Letizia , Netter Antoine , Sari-Minodier Irène , Miquel Laura , Lacan Arnaud , Courbiere Blandine 
Published: November 2022

Abstract
The objective of this study is to explore and describe the professional impact of deep infiltrating endometriosis on infertile women. We conducted a qualitative monocentric study between December 2020 and June 2021. Twelve semi-structured in-depth interviews using a theme-based interview guide with open questions were undertaken with infertile women with deep infiltrating endometriosis. Data analysis was conducted using an inductive approach according to the grounded theory method. Three main themes emerged from the interviews: (i) barriers to reconciling illness and work life, (ii) facilitating factors for well-being at work, and (iii) consequences and outlooks. It appeared that the time of infertility treatment represents a particular period of change in the working lives of women with endometriosis. For most women, these changes are experienced negatively, often with a renunciation of goals. For others, this is the time to communicate the difficulties linked to their illness to their professional entourage. There is a long path ahead to finally achieving recognition of endometriosis in the context of professional life.
Waist-to-Height Ratio Associated Cardiometabolic Risk Phenotype in Children with Overweight/Obesity
Authors: Ukegbu Tochi E. , Wylie-Rosett Judith , Groisman-Perelstein Adriana E , Diamantis Pamela M , Rieder Jessica , Ginsberg Mindy , Lichtenstein Alice H , Matthan Nirupa R , Shankar Viswanathan 
Published: November 2022

Abstract
Background
Higher childhood overweight/obesity has been associated with an elevated risk of insulin resistance and cardiometabolic disorders. Waist-to-height ratio (WHtR) may be a simple screening tool to identify children at risk for cardiometabolic associated obesity. This study investigated whether being in the upper tertile for WHtR predicted the odds of insulin resistance, elevated liver enzyme concentrations, and cardiometabolic risk factor measures using cross-sectional data from the Family Weight Management Study randomized controlled trial.
Methods
Included was baseline data (n = 360, 7–12 years, mean Body Mass Index ≥ 85th percentile for age and sex). WHtR were grouped into tertiles by sex, male: ≤0.55(T1), > 0.55-≤0.59(T2), > 0.59(T3); female: ≤0.56(T1),>0.56-≤0.6(T2), > 0.6(T3). The Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) was used to categorize participants as insulin-resistant (HOMA-IR ≥ 2.6) and insulin-sensitive (HOMA-IR < 2.6). Liver enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were categorized as normal vs. elevated (AST of < 36.0 µkat/L or ≥ 36.0 µkat/L; ALT of < 30.0 µkat/L or ≥ 30.0 µkat/L). We examined differences in baseline cardiometabolic risk factors by WHtR tertiles and sex-specific multivariable logistic regression models to predict IR and elevation of liver enzymes.
Results
Study participants had a mean WHtR of 0.59 ([SD: 0.06)]). Irrespective of sex, children in WHtR T3 had higher BMIz scores, blood pressure, triglycerides, 2-hr glucose, fasting, 2-hr insulin and lower HDL-C concentrations compared to those in T2 and T1. After adjusting for covariates, the odds of elevated IR (using HOMA-IR > 2.6) were over 5fold higher among children in T3 versus T1 (males) and T2 and T3 versus T1 (females). The odds of elevated ALT values (≥ 30) were 2.9 fold higher among female children in T3 compared to T1.
Conclusion
WHtR may be a practical screening tool in pediatric populations with overweight/obesity to identify children at risk of IR and cardiometabolically unhealthy phenotypes in public health settings.
Virtual screening and cheminformatics descriptors uncover insecticidal molecular mechanisms besides plant essential oils' action.
Authors: Corrêa Eduardo José Azevedo , Carvalho Frederico Chaves , Oliveira Júlia Assunção de Castro , Bertolucci Suzan Kelly Vilela , Scotti Marcus Tullius , Silveira Carlos Henrique da , Guedes Fabiana Costa , Melo Júlio Onésio Ferreira , De Melo-Minardi Raquel Cardoso , de Lima Leonardo Henrique Franca 
Published: November 2022

Abstract

Plant essential oils (EOs) are important for discovering new bioactive substances, such as environmentally safe insecticides. However, the high structural diversity of EOs compounds and their elusive biochemical mechanisms make a gap in knowledge of their major targets and action mechanisms. Here we present a predictive cheminformatics methodology and experimental validation of the ability of EO's typical natural compounds to act as potential insecticides against canonical insect molecular targets. A cheminformatics analysis protocol was created to identify chemical signatures of compounds present in EOs capable of interfering with typical targets of insecticides. Three of our clusters presented strong respective signatures for JH-like, Steroids-like and biogenic-amines-like structures, all of them with reported anti-insecticidal effects. Case studies highlight the participation of juvenile hormone-specific proteins (JHBP and MET) and the octopamine receptor at agonist conformation (OctpR ago) as major targets for EOs of typical Brazilian plants. In vivo application of leave’s EOs from Baccharis dracunculifolia on blowflies led to dose-response symptoms in adults, larvae and hatching consonants with such signatures. Taking it all together, the present study points out the success of a promising methodology to identify insecticidal pharmacophore signatures on plants' EOs, which can be of high value for the bioprospection of new natural products and molecules with agro and health interests.
Deletion of Cyclic GMP-AMP Synthase Accelerates Concanavalin A-Induced Acute Hepatic Injury by Facilitating Leukocyte Chemotaxis
Authors: Liu Jiaxin , Ji Shuang , Liu Zhaiyi , Guo Meina , Chen Lihong 
Published: November 2022

Abstract
Growing evidence demonstrates that cyclic GMP-AMP synthase (cGAS), as a cytosolic DNA sensor, is essential for activating innate immunity and regulating inflammatory response against cellular damage. However, its role in immune-mediated hepatitis remains unclear. Here by challenging the cGAS knockout (KO) and their littermate wide-type (WT) mice with intravenous ConA injection to induce acute immune-mediated liver injury, we found that lack of cGAS drastically aggravated the ConA-induced liver damage, reflected by increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and amplified hepatic necrosis. The number of apoptotic hepatocytes was also significantly increased in the KO mice. RNA-sequencing analysis revealed that leukocyte chemotaxis and migration related genes were remarkably upregulated in the KO livers. Consistently, immunofluorescence assays illustrated that the infiltrating F4/80-positive macrophages, Ly6G-positive neutrophils, and CD3-positive T cells were all significantly increased in the KO liver sections. The hepatic expression of the pro-inflammatory genes was elevated as well. Supporting the in vivo findings, knockdown of cGAS in cultured macrophages showed promoted migration potential and enhanced pro-inflammatory gene expression. These results collectively demonstrated that deletion of cGAS could accelerate ConA-induced acute liver injury, and its mechanism might be related to facilitating leukocyte chemotaxis and promoting liver inflammatory response.
WITHDRAWN: Combination of empirical models and observational-based techniques in the quantification of soil loss: a case of Rherhaya watershed, High Atlas, Morocco
Authors: Jadoud Mourad , Achheb Abderrahim El , Laftouhi Noureddine , KHOUZ Abdellah , Trindade Jorge , Bchari Fatima El , Oliveira Sérgio C. , Santos Pedro Pinto , Garcia Ricardo A.C. , Bougadir Blaid , Rachidi Said , Eloudi Hasna , Namous Mustapha , Saouab Tarik 
Published: November 2022

Abstract
The soils are naturally subjected to erosion, this natural and periodic phenomenon shapes the reliefs, under the combined effect of several drivers, and by the intermediary of certain agents such as the water, wind, etc. The eventual result of the rocks' deterioration and wear is a displacement and deposit where the sediments build up. The Rherhaya basin, Western High Atlas, Morocco, is among the areas most affected by erosion, the structure of its reliefs combined with the hierarchy of its rivers, as well as the lithology of the formations that constitute it play in the presence of other aggravating factors, a preponderant role in determining the rate of erosion in the region. Using GIS, the Revised Universal Soil Loss Equation (RUSLE) model, and the Erosion Potential Method (EPM) model, are applied in this study in order to quantify the annual soil losses in the Rherhaya basin. The results obtained by the RUSLE model are very consistent with the results measured, both in terms of volume, between 2.5 and 6.9 t/ha/yr as soil loss average volume observed versus 4.07 t/ha/yr calculated and 340 t/ha/year as the maximum volume observed versus 317.86 t/ha/year calculated. The maximum soil loss of 542 t/ha/yr and the average soil loss of 103 t/ha/yr are both overestimated by the EPM model, compared to the observed data.
Vaccination of SARS-CoV-2-infected individuals expands a broad range of clonally diverse affinity-matured B cell lineages
Authors: Chernyshev Mark , Sakharkar Mrunal , Connor Ruth , Dugan Haley , Sheward Daniel , Rappazzo C. Garrett , Stålmarck Aron , Forsell Mattias , Wright Peter , Corcoran Martin , Murrell Ben , Walker Laura , Hedestam Gunilla Karlsson 
Published: November 2022

Abstract
Vaccination of SARS-CoV-2 convalescent individuals generates broad and potent antibody responses. Here, we isolated 459 spike-specific monoclonal antibodies (mAbs) from two individuals who were infected with an early ancestral strain of SARS-CoV-2 and later boosted with mRNA-1273. We characterized mAb genetic features by sequence assignments to the donors’ personal immunoglobulin genotypes and assessed antibody neutralizing activities against ancestral SARS-CoV-2, Beta, Delta, and Omicron variants. The mAbs used a broad range of immunoglobulin heavy chain (IGH) V genes in the response to all sub-determinants of the spike examined, with similar characteristics observed in both donors. IGH repertoire sequencing and B cell lineage tracing at longitudinal time points revealed extensive evolution of SARS-CoV-2 spike-binding antibodies from acute infection until vaccination five months later. These results demonstrate that highly polyclonal repertoires of affinity-matured memory B cells were efficiently recalled by vaccination, providing a basis for the potent antibody responses observed in convalescent persons following vaccination.
Temozolomide combined with Radiotherapy can benefit patients with glioma: A meta-analysis of 10 randomized controlled trials
Authors: huang Qinhong , Yang Lihui , Ge Fan , Ye Zhenwei , Ke Yiquan 
Published: November 2022

Abstract
Background
Glioma originates from glial cells of the nervous system and is the most common malignant tumor in the brain. Temozolomide is considered as a promising medicine that can improve the overall survival (OS) and progress-free survival (PFS) rate of patients with glioma after operation. However, whether radiotherapy plus temozolomide has substantive role for glioma patients remains controversial.
Methods
We conducted a meta-analysis included 10 Randomized controlled trials (RCTs) encompassing 2703 patients to evaluate the efficacy and safety of radiotherapy plus temozolomide for glioma. EMbase, PubMed, Web of Science, and Cochrane library were searched. Our study strictly follow the PRISMA guideline.
Results
Overall, compared with receiving radiotherapy alone, patients through radiotherapy combined with temozolomide has substantial benefit on both OS (HR = 0.78; 95% CI: 0.62–0.98; P = 0.03) and PFS (HR = 0.62; 95% CI: 0.49–0.78; P < 0.0001). The best effect shows in patients receiving radiotherapy combined with both concurrent and adjuvant temozolomide on both OS (HR = 0.66; 95% CI: 0.58–0.74; P < 0.00001) and PFS (HR = 0.55; 95% CI: 0.48–0.62; P < 0.00001), when compared with radiotherapy alone. Whereas, temozolomide monotherapy do not show greater benefits than radiotherapy alone on OS (HR = 0.93; 95% CI: 0.74–1.14; P = 0.53). Moreover, radiotherapy plus temozolomide obviously increase the incidence of hematological complications than only radiotherapy (RR = 10.31; 95% CI: 4.49–23.71; P < 0.00001). Also, our meta-analysis suggested that MGMT methylation test can be helpful for evaluating prognosis, determining individualized treatment and forecasting the curative effect.
Conclusion
Radiotherapy combined with temozolomide, especially combined with both concurrent and adjuvant temozolomide has great benefit on both OS and PFS of glioma patients. Conditionally, patients with glioma receiving glioma MGMT methylation test have advantage over evaluating prognosis, determining individualized treatment and forecasting the curative effect. Last but not least, monitoring blood routine and remedying the possible abnormalities in time is especially necessary of patient receiving temozolomide therapy.
PRMT5 methylating Smad4 activates TGF-β signaling and promotes colorectal cancer metastasis
Authors: Wang Guihua , Liu Anyi , Yu Chengxin , Qiu Cheng , Wu Qi , Huang Changsheng , Li Xun , She Xiaowei , Wan Kairui , Liu Lang , Li Mao , Wang Zhihong , Chen Yaqi , Hu Fuqing , Song Da , Li Kangdi , zhao chong , Deng Haiteng , Sun Xuling , Xu Feng , Lai Senyan , Luo Xuelai , Hu Junbo 
Published: November 2022

Abstract

Perturbations in transforming growth factor- β (TGF-β) signaling can lead to a plethora of diseases, including cancer. Mutations and posttranslational modifications (PTMs) of the partner of Smad complexes contribute to the dysregulation of TGF-β signaling. Here, we reported a PTM of Smad4, R361 methylation, that was critical for Smad complexes formation and TGF-β signaling activation. Through mass spectrometric, co-immunoprecipitation (Co-IP) and immunofluorescent (IF) assays, we found that oncogene protein arginine methyltransferase 5 (PRMT5) interacted with Smad4 under TGF-β1 treatment. Mechanically, PRMT5 triggered Smad4 methylation at R361 and induced Smad complexes formation and nuclear import. Furthermore, we emphasized that PRMT5 interacting and methylating Smad4 was required for TGF-β1-induced epithelial-mesenchymal transition (EMT) and colorectal cancer (CRC) metastasis, and Smad4 R361 mutation diminished PRMT5 and TGF-β1-induced metastasis. In addition, highly expressed PRMT5 or high level of Smad4 R361 methylation indicated worse outcomes in clinical specimens analysis. Collectively, our study highlights the critical interaction of PRMT5 and Smad4 and the roles of Smad4 R361 methylation for controlling TGF-β signaling during metastasis. We provided a new insight for Smad4 activation. And this study indicated that blocking PRMT5-Smad4 signaling might be an effective targeting strategy in Smad4 wide type CRC.
Combined COVID-19 vaccination and hepatitis C virus screening intervention in marginalised populations in Spain
Authors: Lazarus Jeffrey , Villota-Rivas Marcela , Ryan Pablo , Buti Maria , Grau-López Lara , Cuevas Guillermo , Espada José Luis , Morón William , Palma-Álvarez Raul Felipe , Feld Jordan , Valencia Jorge 
Published: November 2022

Abstract
Background: COVID-19 has hindered HCV and HIV screening, particularly in marginalised groups, who have some of the highest rates of these conditions and lowest rates of COVID-19 vaccination. We assessed the acceptability of combining HCV testing with COVID-19 vaccination in a centre for addiction services (CAS) in Barcelona and a mobile testing unit (MTU) in Madrid, Spain.
Methods: From 28/09/2021-30/06/2022, 187 adults from marginalised populations were offered HCV antibody (Ab) testing along with COVID-19 vaccination. If HCV Ab+, they were tested for HCV-RNA. MTU participants were screened for HIV, per the standard of care. HCV-RNA+ and HIV+ participants not on ART were offered treatment.
Results: Findings show how of the 86 CAS participants: 93% had been previously vaccinated for COVID-19, of whom 90% had the full first round schedule; none had a COVID-19 vaccine booster and all received a COVID-19 vaccine; 62.8% were tested for HCV Ab, of whom 31.5% were positive, of whom all were tested for HCV-RNA and none were positive. Of the 101 MTU participants: none had been vaccinated for COVID-19 and all received a COVID-19 vaccine; all were tested for HCV Ab and HIV and 14.9% and 8.9% were positive, respectively; of those HCV Ab+, nine (60%) were HCV-RNA+, of whom eight (88.9%) have started treatment; five (55.6%) of those HIV+ had abandoned ART, of whom three (60%) have re-started it.
Conclusions: The intervention had an acceptability rate of 62.8% at the CAS and 100% at the MTU and can be used in marginalised communities.
Inflammatory bowel disease and rosacea: causal association analysis using bi-directional Mendelian randomization
Authors: Li Min , He Si Xian , He Yuan Xiong , Hu Xiao Han , Zhou Zhou 
Published: November 2022

Abstract
Background:
The association between rosacea and inflammatory bowel disease (IBD) has been studied in previous observational studies. It is unclear, however, whether the association is causal or not.
Methods:
Independent genetic variants for IBD were chosen as instruments from published GWAS studies involving 38155 cases with an IBD diagnosis and 48485 controls in order to investigate the causal effect of IBD on rosacea. Summarized data for rosacea were gathered from various GWAS studies that included 1195 cases and 211139 controls without rosacea. Reverse-direction MR analysis was done to investigate the relationship between genetically proxied rosacea and IBD. With the use of the inverse variance-weighted (IVW), MR-Egger, and weighted median approaches, a 2-sample Mendelian randomization study was carried out. Analysis of heterogeneity and sensitivity was performed to examine the pleiotropy and robustness of effect estimates.
Results: The forward-direction of the MR study was to reveal that genetic predisposition to IBD (OR: 1.1291; 95% CI: 1.0444 to 1.2206), UC (OR: 1.2030; 95% CI: 1.0867 to 1.3318) and CD (OR: 1.1291; 95% CI: 1.0444 to 1.2206; p=0.0023) was associated with an increased risk of rosacea. The reverse-direction MR analyses did not demonstrate that a genetic predisposition to rosacea was not associated with total IBD (OR: 0.9683; 95% CI: 0.9112 to 1.0291), UC (OR: 0.9714; 95% CI: 0.8949 to 1.0545) and CD (WM: OR: 0.9751; 95% CI: 0.8971 to 1.0598; p=0.5525).
Conclusion: Our findings provided evidence for a causal impact of IBD, UC, and CD on rosacea, but not vice versa. The elevated incidence of rosacea in patients with IBD should be recognized by doctors to make an early diagnosis and initiate specialized therapy.