bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of novel enhancer RNA biomarkers and corresponding prognostic signatures of hepatocellular carcinoma
Authors: Chen Peng , Qi Debin , Sun Keyan , Song Shaohua , Li Tao 
Published: September 2022

Abstract
Background
Researchers now pique an interest on enhancer RNAs (eRNAs). Their roles in hepatocellular carcinoma (HCC) remain to be elucidated. Further investigation on eRNAs may shed light on putative prognosis biomarkers and therapeutic targets, which may benefit for early diagnosis of HCC.
Methods
RNA-Seq data and clinical information were obtained from TCGA cohort. Data were analyzed using R software. Prognosis-related eRNAs were identified, and their target genes of eRNAs were predicted based on the correlation level. We performed enrichment and pan-cancer analyses, constructed prognostic model using selected eRNAs and target genes. QPCR was performed for validation.
Results
125 prognosis-related eRNAs were identified. 15 eRNA and target gene pairs were selected for the association with HCC tumorigenesis-related biological behaviors. We chose AL137803.1 for further investigation. We revealed target genes, C5orf58, NDC1, LINC00501 and G6PD could be prognostic signatures of HCC. The prediction value was confirmed and pan-cancer analyses also demonstrated multiple function of the key eRNA and its target genes. Our hypothesis was validated eventually.
Conclusion
A novel eRNA AL137803.1 may be a potential biomarker of HCC, besides its putative target genes may serve as prognostic signatures.
Pattern-Based Hybrid Book Recommendation System using Semantic Relationships
Authors: Wayissa Fekadu , Leranso Mesfin , Asefa Girma , Kedir Abduljebar , Salau Ayodeji Olalekan 
Published: September 2022

Abstract
In the fields of machine learning and artificial intelligence, recommendation systems (RS) or recommended engines are commonly used. In today's world, recommendation systems based on user preferences assist consumers in making the best decisions without depleting their cognitive resources. They can be applied to a variety of things, including search engines, travel, music, movies, literature, news, gadgets, and dining. A lot of people utilize RS on social media sites like Facebook, Twitter, and LinkedIn, and it has proven beneficial in corporate settings like those at Amazon, Netflix, Pandora, and Yahoo. There have been numerous proposals for recommender system variations. However, certain techniques result in unfairly recommended things due to biased data because there are no established connections between the items and consumers. In order to solve the challenges mentioned above for new users, we propose in this work to employ Content-based Filtering (CBF) and Collaborative Filtering (CF) with semantic relationships to capture the relationships as knowledge-based book recommendations to readers in a digital library. When proposing things, patterns are more discriminative than single phrases. To capture the similarity of the books that the new user had retrieved, the patterns were grouped in a semantically equivalent manner using the Clustering method. The effectiveness of the suggested models is examined through a series of extensive tests employing Information Retrieval (IR) evaluation criteria. Recall Precision and F-Measure, two of the three widely used performance measuring metrics, were employed. The findings demonstrate that the suggested model performs noticeably better than cutting-edge models.
Outcomes of surgical revascularization for pediatric moyamoya disease and syndrome
Authors: Boulter Jason H , Szuflita Nicholas S , Keating Robert F , Magge Suresh N 
Published: September 2022

Abstract

Purpose: Moyamoya disease and syndrome represent rare entities characterized by progressive stenosis and/or occlusion of the intracranial blood vessels. We present our series of patients with moyamoya disease and syndrome stratified by underlying disease and analyze differences in presentation and outcome following surgical revascularization.Methods: This was an Institutional Review Board (IRB) approved, retrospective review of all patients surgically revascularized by the senior author (SNM) while at Children’s National Hospital in Washington, DC. Demographic data, presenting symptoms and severity, surgical details, and functional and radiographic outcomes were obtained and analyzed for differences amongst the underlying cohorts of moyamoya disease and syndrome as well as by unilateral or bilateral disease and index or non-index surgeries.Results: Twenty-two patients were identified with the following underlying diseases: six with idiopathic moyamoya disease, six with sickle cell anemia, five with trisomy 21, and five with neurofibromatosis type 1. Thirty hemispheres were revascularized with a significantly reduced rate of stroke from 3.19 strokes/year (SD = 3.10) to 0.13 strokes/year (SD = 0.25), p = 0.03. When analyzed by underlying cause of moyamoya syndrome, patients with neurofibromatosis type 1 were found to be significantly less likely than the other subtypes of moyamoya syndrome to have had either a clinical stroke (0.0% vs 100.0% [sickle cell], 60.0% [trisomy 21], or 83.3% [moyamoya disease], p < 0.01) or radiographic stroke (0.0% vs 100.0% [sickle cell], 60.0% [trisomy 21], or 83.3% [moyamoya disease], p < 0.01) at time of presentation. Additionally, patients with bilateral disease demonstrated no difference in final functional outcome (mRS 0.73 (SD = 1.33) vs. 1.29 (SD = 1.60), p = 0.63), but second, contralateral surgeries were associated with an increased rate of temporary peri-operative ischemic events when compared to index procedures (50.0% vs 9.1%, p = 0.03).Conclusion: Surgical revascularization decreases stroke risk for pediatric patients with different forms of moyamoya disease and moyamoya syndrome. In this study, all but one patient underwent pial synangiosis for indirect revascularization.
Nucleation Front Proliferation in Bi-modal PCBM Crystals and a Crystal Fan – Axial Morphological Transition.
Authors: Hynes Elizabeth 
Published: September 2022

AbstractThis paper analyses crystal properties of PCBM when annealed in a bilayer with PS on the top. There are intriguing formations and definite thresholds to the varying reproducible morphologies found. This paper suggests that the PS layer greatly influences PCBM crystal nucleation rate and final morphology.
Evaluation of a Transition to Survivorship Program for Pediatric, Adolescent and Young Adult Cancer Patients and Caregivers
Authors: Bingen Kristin , Karst Jeffrey , Anderson Lynnette , Chan Sherilynn , Jordan Anna , Morin Jocelyn , Nichols Julie , Palou-Torres Akasha , Phelan Rachel , Schmidt Debra , Yan Ke , Hoag Jennifer A 
Published: September 2022

Abstract
Purpose
Survivorship education and anticipatory guidance represents an unmet need for pediatric, adolescent, and young adult (AYA) cancer survivors and their caregivers when treatment ends. This pilot study evaluated the feasibility, acceptability, and preliminary efficacy of a structured transition program, bridging treatment to survivorship, to reduce distress and anxiety and increase perceived preparedness for survivors and caregivers.
Methods
Bridge to Next Steps is a 2-visit program, within 8 weeks of treatment completion and 7 months post-treatment, which provides survivorship education, psychosocial screening, and resources. Fifty survivors and 46 caregivers participated. Participants completed pre- and post-intervention measures: Distress Thermometer and PROMIS anxiety/emotional distress (ages ≥ 8 years), and perceived preparedness survey (ages ≥ 14 years). AYA survivors and caregivers completed a post-intervention acceptability survey.
Results
Most participants (92.59%) completed both visits, and most AYA survivors (57.1%) and caregivers (76.5%) endorsed the program as helpful. Caregivers’ distress and anxiety scores decreased from pre- to post-intervention (p < 0.01). Survivors’ scores remained the same, which were low at baseline. Participants felt more prepared for survivorship from pre- to post-intervention (p = 0.02, p < 0.01, respectively).
Conclusions
Bridge to Next Steps was feasible and acceptable for most participants. AYA survivors and caregivers felt more prepared for survivorship care after participation. Caregivers reported decreased anxiety and distress from pre- to post-Bridge, whereas survivors remained at a low level for both.
Implications for Cancer Survivors:
Effective transition programs that better prepare and support pediatric and AYA cancer survivors and families from active treatment to survivorship care will contribute to healthy adjustment.
Corneal Endothelial Cell insult by Chronically Elevated Intraocular Pressure in Primary Open Angle Glaucoma: A Cross-sectional Study
Authors: A.Mateen Elshimaa , Sayed Khulood Muhammad , Mounir Amr , Ammar Hatem Gamal 
Published: September 2022

Abstract
PURPOSE: To evaluate specular microscopic changes of corneal endothelial cell (CEC) count and morphology in correlation to retinal nerve fiber layer RNFL changes detected by OCT in early and advanced primary open-angle glaucoma (POAG).METHODS: A cross-sectional study was conducted on patients with POAG versus non-glaucomatous patients of the same age group. Specular microscopy, VF test, OCT scans of RNFL, and macular ganglion cell complex (GCC) were performed. Glaucoma group was further subdivided into early and advanced stages.RESULTS: The study included 130 eyes of 130 subjects, 70 were eyes with POAG (early glaucoma 40 eyes, advanced glaucoma 30 eyes), 60 were healthy eyes (control group). Both groups were comparable regarding mean age and sex.In the early glaucoma group, a significant negative correlation was found between the coefficient of variation (CV%) and superior RNFL thickness (r=-0.5, p-value=0.018). A significant negative correlation was also found between percentage of cellular hexagonality (HEX%) and vertical cup/disc (C/D) ratio (r=-0.43, p-value= 0.035). A significant positive correlation was found between HEX% and (superior, inferior) RNFL thickness (r=0.53, 0.5 and p-value= 0.008, 0.015) respectively.Mean CEC count was significantly lower in the advanced glaucoma group than in the control group (2958.7±371.2 vs 3085±172.5, p value=0.043). CONCLUSIONS: CEC is affected by chronic intraocular pressure (IOP) elevation just like the RNFL and macular GCC. CEC morphology is insulted in the early stages of POAG while the count can withstand chronic IOP elevation till advanced stages where both are affected.
Trace Metals Assessment in Soils and Sediments near the Abandoned Mine of “El Abed” Pb-Zn deposit – Northwestern Algeria
Authors: Mellah Farida , Boutaleb Abdelhak , Henni Bachir , Dalila Berdous , Abdelhamid Mellah 
Published: September 2022

Abstract
El-Abed is one of the largest historical and abandoned mining operations for Pb–Zn deposits in northwestern Algeria, leaving large amounts of mining wastes accumulating under the influence of wind erosion. This study aims to verify the concentrations and sources of heavy metals for fifty-six (56) surface samples of environmental media (soil, sediments and mining residues).Chemical analyzes were performed using an optical emission spectrophotometer. A set of environmental quality indicators and multivariate statistical methods with geographic information system was obtained. In this study the mean values ​​of the concentrations of trace metals are: (As = 30,82), (Pb = 1219,27), (Zn = 2855,94), (Cu = 5,3),mg/Kg, and the order as following : Zn > Pb > As > Cu. Based on these results, all trace metals except Copper (Cu) bypassed the the Geochemical background value in the Earth's crust. The results of the multivariate census indicated that there is a strong relationship for pollution indicators for trace elements lead (Pb), zinc (Zn) and arsenic (As). This factors indicate that they are from human sources, and a weak relationship for pollution indicators for trace elements copper (Cu), which is a natural source. Using Geographic Information System and based on spatial distribution maps, many hotspots have been identified. All sampling sites indicate general pollution, poor site quality and environmental hazards.
Bridging the Gap, How Interprofessional Collaboration Can Support Family-Centered Emergency Preparedness: An Exploratory Qualitative Study
Authors: Flanagan Shelby K. , Sterman Julia J. , Merighi Joseph R. , Batty Rachael 
Published: September 2022

Abstract
BACKGROUND
Children with disabilities and their families are at higher risk during emergencies and disasters, which is often attributed to the lack of disability inclusion in emergency response as well as disparities in preparedness. This disparity speaks to a need for emergency preparedness that centers children with disabilities and their families. The goal of this study was to provide recommendations for practice and policy to better support family-centered emergency preparedness inclusive of children who have disabilities, with the ultimate objective of improving safety outcomes for children with disabilities and their families in emergency situations.
METHODS
This study consisted of 46 qualitative interviews with nurses, occupational therapists, social workers, public safety professionals, and advocacy organization representatives about their role in emergency preparedness for families of children with disabilities. Qualitative content analysis was used to identify themes from participants’ responses.
RESULTS
Participants expressed interest in family-centered emergency preparedness, and stated that greater awareness, more education and training, increased networking between professions, and institutional support would enable their involvement.
CONCLUSIONS
These findings have implications for the importance of interprofessional collaboration in supporting family-centered emergency preparedness for families of children with disabilities. Stronger interprofessional networks would help overcome many of the barriers identified by participants, and advocacy groups appear to be well-positioned to bridge the gap between these professionals and their areas of expertise.
Comparative proteome analysis illustrates tobacco defense mechanisms in response to Ralstonia solanacearum infection
Authors: Sun Yiwen , Si Huan , Alariqi Muna , Ali Qurban , Chen Luo , Wei Hao , Zhao Liyan , Yang Guangqin , Jin Shuangxia , Cao Jinglin 
Published: September 2022

Abstract
Background
Ralstonia solanacearum causing bacterial wilt disease provokes tremendous losses in global crop production including tobacco. Transcriptional changes in response to R. solanacearum infection have already been intensively studied in various plant species. However, the genetic networks regulating defense responses of different tobacco varieties having different resistance backgrounds are quite limited.
Results
In this study, we performed a comparative proteome analysis of two tobacco cultivars, resistant (R) and susceptible (S), during the infection of R. solanacearum, to uncover the potential candidate genes regulating tobacco resistance to R. solanacearum. We observed huge difference in protein profiles between the two cultivars after R. solanacearum infection. The proteins involved in salicylic acid (SA), jasmonic acid (JA) and ethylene (ETH) exhibited significant changes in response to R. solanacearum. These changes thought to regulate defense responses in tobacco plants. Phenylpropanoid metabolism related proteins including PAL, 4CL, POD, and TOGT also showed differential regulation in both R and S tobacco cultivars in response to R. solanacearum infection, which may be one of the reason why the different resistance showed in these two cultivars. By using CRISPR/Cas9, we experimentally validated that knockout of NtTOGT gene has decreased tobacco resistance to R. solanacearum.
Conclusion
Collectively, our results provide new insights to understand tobacco defense mechanism against R. solanacearum that would benefit breeding programs in the future. It will asset to identify potential defense responsive genes to understand and improve plant resistance. In addition, we suggest that NtTOGT might be a resistant gene involved in plant defense response against R. solanacearum.
Intensified tuberculosis treatment to reduce the mortality of HIV-infected and uninfected patients with tuberculosis meningitis (INTENSE-TBM): Study protocol for a Phase III Randomized Controlled Trial
Authors: Bonnet Fabrice 
Published: September 2022

Abstract
Background: Tuberculous meningitis (TBM) is the most lethal and disabling form of tuberculosis (TB) particularly in sub-Saharan Africa. Current anti-TB treatment is poorly effective since TBM mortality reaches 40% in HIV negative patients and up to 70% in HIV co-infected patients. To reduce TBM induced morbidity and mortality, the INTENSE-TBM trial evaluates two interventions in both HIV-infected and uninfected patients: an anti-TB treatment intensification using oral high-dose rifampicin (35 mg/kg daily) and linezolid (1,200 mg daily and then 600 mg daily) during the first 8 weeks of the anti-TB treatment and the use of adjunctive aspirin (200 mg daily).Methods: This is a randomized controlled, phase III, multicenter, 2 x 2 factorial plan superiority trial. The trial has four arms, combining the two experimental treatments (intensified TBM regimen and aspirin) with the two reference treatments (WHO standard TB treatment and placebo) and is open-label for anti-TB treatment and double blind placebo-controlled for aspirin treatment. This trial is conducted in adult or adolescent of age ≥15 years with TBM defined as “definite”, “probable” or “possible” using Tuberculosis Meningitis International Research Consortium criteria, in four African countries: Ivory Coast, Madagascar, Uganda and South Africa. The primary outcome is all-cause death between inclusion and week 40.Discussion: The INTENSE-TBM trial represents a key opportunity to enhance TBM treatment with widely available existing drugs notably in high incidence setting of both TB and HIV. The trial design is pragmatic and the results will permit early and effective applications in TBM patients care, in both HIV and TB high incidence countries.Trial registration: ClinicalTrials.gov, ID: NCT04145258Trial Registration Dataset: Table 1
X-photon laser direct write 3D nanolithography
Authors: Malinauskas Mangirdas , Skliutas Edvinas , Samsonas Danielius , Čiburys Arūnas , Kontenis Lukas , Gailevicius Darius , Narbutis Donatas , Jukna Vytautas , Vengris Mikas , Juodkazis Saulius 
Published: September 2022

Abstract
Polymerized voxels by focusing ultrashort pulses varying wavelength over three-octaves wide range for femtosecond laser direct write 3D nanoprinting are studied. A possibility to use any color of spectrum from 500-nm-to-1200-nm with a controlled pulse width of 100-fs reveals delicate interplay of photo-physical mechanisms more than just two-photon absorption inducing localized photo-polymerization. None of the applied exposure conditions ensured significantly higher spatial definition than 200-nm in width using SZ2080TM photosensitized and pure materials, yet it allowed tuning the voxel aspect ratio and greatly influenced dynamic fabrication window determined by the resolution bridges method. An effective order of absorption, i.e., the nef-photon process of absorption, and exposure per pulse (energy, fluence, intensity) was assessed for ultra-short pulses varying wavelengths as it has the paramount influence to the established multi-photon polymerization (MPP) process. Here an evolution of the polymerised volume via different energy delivery mechanisms: one-/two-/three-photon absorption, avalanche ionization, thermal diffusion leading to controlled photo-polymerization are revealed. The results can be used to tailor polymerized volume for increasing the 3D nano-printing performance. A non-trivial energy deposition by X-photon absorption observed with an onset of a strong lateral size increase at the higher pulse energy at longer wavelengths and can be understood as due to reaching epsilon-near-zero conditions. Findings are valuable for the developing MPP technology to reduce the footprint size and increase its efficiency. Understanding mechanisms and appearance of λ-tunable commercial lasers are benefiting broad applications in advanced optical additive manufacturing areas of micro-optics, nano-photonic devices, meta-materials, and integrated-chips, and tissue engineering.
Evaluation of the effectiveness of probiotics in the treatment of urinary tract infection among women in Bangladesh
Authors: Farhana Fatima , Mosaddek Nafisa , Islam Md. Saiful , Hossain Nazmul , Sarker Anima , Mukta Taposhi Rabeya , Akter Rumi , Rizvi Rinat , Rakibul Hasan K.M. , Ansary E A F , Mosaddek Abu Syed 
Published: September 2022

Abstract
Background: Urinary tract infection (UTI) occurs more in women than men. Escherichia coli are responsible in most cases. The microbial (Lactobacillus) species which inhabit in the vaginal tract, play an important role in the prevention of infection. Irrational use of antibiotics contributes to changes in antibiotic susceptibility patterns of pathogens. Hence, administration of probiotics exogenously may cause colonization of Lactobacillus in female urogenital organs.Objectives: The study aimed to assess the effectiveness of probiotics in the treatment of UTI among women in Bangladesh.Methods: This interventional, prospective, non-randomized trial was conducted among women suffering from UTI in the department of Pharmacology and Gynecology of Uttara Adhunik Medical College Hospital, Bangladesh from January, 2019 to June, 2021. A total of 162 women who suffered from UTI were enrolled to receive probiotic, antibiotic, and probiotic + antibiotic treatments. Urine culture and sensitivity test of all participants was done during enrollment. Patients came for follow-up visits on day 30 of completion of therapy and the final study population was 132. Results: Among the 132 participants, the mean ages were 34.23±14.28 years (mean±SD). Average duration of UTI prior to enrollment was 77.61±288.59 days. 93.18% patients complaint burning sensation of urination, pain or pressure in the back or lower abdomen (67.42%) and fever (37.12%), respectively. During enrollment, urine R/M/E test revealed that most of the urine samples showed no growth of organisms (84.8%) and E. coli (09.85%). Most commonly used antibiotic was nitrofurantoin (43.33%), cefixime (18.88%), and ciprofloxacin (18.88%). During follow-up, urine sample culture revealed no growth of pathogenic organisms in 131 (99.24%) and E. coli in one patient who was treated with only probiotics.Conclusions: The present study revealed that the use of probiotics is effective and safe to treat UTI in women in Bangladesh.Trial registration number: CTRI/2020/05/025175, Date: 14.05.2020