bluuebunny/crossref_metadata_2025_split huggingface dataset:
ZnO electron transporting layer engineering realized over 20% efficiency and over 1.28 V open‐circuit voltage in all‐inorganic perovskite solar cells
Authors: Wu Xiao , Zhang Jianquan , Qin Minchao , Liu Kuan , Lv Ziyu , Qin Zhaotong , Guo Xinlu , Li Yuhao , Xu Jianbin , Li Gang , Yan He , Lu Xinhui 
Published: February 2022

AbstractCesium lead based all‐inorganic perovskite solar cells (PSCs) are promising candidates for tandem solar cells owing to their favorable thermal stability and suitable bandgap. However, to exploit the advantage to the full, there is still huge room to reduce energy loss and enhance the VOC. In this work, we developed low‐temperature processed ZnO as an ETL alternative. Incorporation of PbX2 (X = I−, Cl−, and CH3COO−) was found to increase oxygen vacancies in ZnO, which not only promoted the growth of all‐inorganic perovskite layer with improved morphology and orientational order, but also formed a favorable energy level alignment with the perovskite layer, thus facilitating charge extraction and suppressing charge recombination. Consequently, the CsPb(I1−xBrx)3 PSCs based on PbX2:ZnO exhibited enhanced stability and performance. The efficiency was boosted from 17.64% to 20.04% with an improved fill factor of 84.14% and a VOC of 1.28 V, being the highest VOC among highly efficient (>20%) all‐inorganic PSCs to date.
Statistical study on soot volume fractions of buoyant turbulent diffusion fires
Authors: Bi Yubo , Lu Shouxiang , Gao Wei , Bi Mingshu , Cong Haiyong 
Published: February 2022

SummaryThe statistical soot properties of buoyant turbulent diffusion propane fire were experimentally and theoretically studied with different fuel sooting tendencies by nitrogen addition. Results show that nitrogen addition decreases the instantaneous soot volume fraction and increases soot intermittency. The combination of these two factors together leads to a decrease in mean soot volume fraction when nitrogen is added. Both radially integrated and the total soot volume fraction decrease with nitrogen addition. The radially integrated soot volume fraction is found to be a function of fuel concentration. A dimensionless correlation is proposed to predict the total soot volume fraction based on theoretical analysis and in good agreement with the experimental data. The results could provide guidance for predicting soot yield for buoyant turbulent fires.
The influence of two yeast strains on fermentation and flavour composition of cider
Authors: Tangüler Hasan , Erten Hüseyin 
Published: March 2022

AbstractTwo commercial dry strains of Saccharomyces cerevisiae and their different inoculum levels were used to carry out cider fermentation to assess their impact on the quality and volatile flavour composition of cider. Analyses indicated that the composition and production of flavour compounds of ciders are generally significantly affected by the strains and inoculation levels. Additionally, the Uvaferm CM strain produced the lowest amount of relative and total flavour compounds compared to Actiflore PM. According to the PCA, differences in volatile components were observed in ciders obtained from both yeasts. While the 1st and 2nd concentrations of Actiflore PM yeast fall to the same region and the others to the different region, the 1st, 2nd and 3rd concentrations of the Uvaferm CM yeast are located in the same region and the others in the different region. It could be said that the Actiflore PM strain synthesizes compounds (such as ethyl alcohol, relative and total esters) that are effective on the quality of cider, the higher amount that Uvaferm CM. The data obtained can be useful for cider producers.
Response surface optimization of pudding formulation containing fish gelatin and clove (Syzygium aromaticum) and cinnamon (Cinnamomum verum) powder: Effect on color, physicochemical, and sensory attributes of the final pudding product
Authors: Choobkar Nasrin , Daraei Garmakhany Amir , Aghajani Abdolraza R. , Ataee Maryam 
Published: February 2022

AbstractIn this study, the response surface methodology (RSM) was used to optimize the pudding formulation ingredients including the fish/bovine gelatin ratio and cinnamon and clove powder and determine the color and physicochemical and sensory attributes’ change in final pudding product. Experiments were carried out based on a central composite design (CCD). The results showed that by increasing the ratio of fish gelatin to bovine gelatin (FG/BG) up to 3%, the moisture content increased slightly and then decreased significantly. Increasing the cinnamon powder to 0.5% reduced the moisture content. Increasing the FG/BG in the formulation of pudding samples reduced the protein content. The effects of cinnamon and clove powder on the protein content were increasing and decreasing, respectively. By increasing the FG/BG ratio, the samples syneresis showed a significant decrease, while the effects of cinnamon and clove powder on the syneresis were nonsignificant. As the level of cinnamon and clove powder increased, the L* value decreased. Cinnamon and clove powder had a linear effect, and the interaction of gelatins and clove powder had a significant effect on changes in redness. The effects of cinnamon and clove powder on b* value were significant. In terms of sensory evaluation, increasing the cinnamon powder concentration increased the appearance scores, while in the case of fish gelatin, this trend was downward. The linear effect of cinnamon powder on taste was significant, while other variables had no significant effect on the taste of the samples. The sample texture was significantly affected by fish gelatin and clove powder. Increasing FG/BG from 0% to 2.5% increased the texture score, but after this range, a decrease in the texture score was observed. The overall acceptance of samples was more affected by spice powder compared to gelatin. By increasing the cinnamon powder and FG/BG, the overall acceptance increased and decreased, respectively. In conclusion, the optimal FG/BG and cinnamon and clove powder were introduced 1.479%, 0.288%, and 0.619% respectively.
N‐terminus DUX4‐immunohistochemistry is a reliable methodology for the diagnosis of DUX4‐fused B‐lymphoblastic leukemia/lymphoma (N‐terminus DUX4 IHC for DUX4‐fused B‐ALL)
Authors: Siegele Bradford J. , Stemmer‐Rachamimov Anat O. , Lilljebjorn Henrik , Fioretos Thoas , Winters Amanda C. , Dal Cin Paola , Treece Amy , Gaskell Alisa , Nardi Valentina 
Published: February 2022

AbstractB‐lymphoblastic leukemia/lymphoma (B‐ALL) is the most common pediatric malignancy and the most commonly diagnosed adult lymphoblastic leukemia. Recent advances have broadened the spectrum of B‐ALL, with DUX4 gene fusions implicated in a subclass occurring in adolescents and young adults and harboring a favorable prognosis. DUX4 fusions have been challenging to identify. We aimed to determine whether expression of the DUX4 oncoprotein, as detected by targeted immunohistochemistry, might serve as a surrogate for molecular detection of DUX4 fusions in B‐ALL. A cohort of investigational B‐ALLs was generated with enrichment for DUX4 fusions by the inclusion of cases with characteristic demographic features and immunophenotypic properties. B‐ALLs with mutually exclusive cytogenetics were collected. Immunohistochemical staining by a monoclonal antibody raised against the N‐terminus of the DUX4 protein was performed. N‐DUX4 immunohistochemistry demonstrated strong, crisp nuclear staining in blasts of seven investigational cases, six of which had nucleic acid material available for molecular evaluation. Five of these cases demonstrated RNA‐seq DUX4‐fusion positivity. One N‐DUX4 immunohistochemistry positive case lacked a definitive DUX4‐fusion by RNA‐seq, though demonstrated a gene expression profile characteristic of DUX4‐rearranged B‐ALLs, a CD2+ immunophenotype, and a lack of staining by C‐terminus DUX4 antibody immunohistochemistry. At least 83.3% [5/6] positive predictive value. N‐DUX4 immunohistochemistry was negative in blasts of three RNA‐seq DUX4‐fusion‐negative cases (3/3; 100% negative predictive value). B‐ALLs with mutually exclusive cytogenetic profiles were all N‐DUX4 negative (0/10, specificity 100%). N‐DUX4 immunohistochemistry is reliable for the distinction of DUX4‐rearranged B‐ALLs from other B‐ALLs. We recommend its use for subclassification of B‐ALLs in adolescents and young adults and in B‐ALLs that remain “not otherwise specified.”
The pharmacist's responsibility to ensure appropriate use of direct‐to‐consumer genetic testing
Authors: Gammal Roseann S. , Smith D. Max , Wiisanen Kristin W. , Cusimano Joseph M. , Pettit Rebecca S. , Stephens Julie W. , Walko Christine M. , Yang Rachel Hagyeong , Dunnenberger Henry M. 
Published: March 2022

AbstractMillions of consumers have chosen to pursue direct‐to‐consumer (DTC) genetic testing for personal health‐related and/or non‐health‐related information. However, genetic test results can be complex to interpret, and the regulatory landscape for DTC genetic tests is in flux. Given that these tests are easily accessible and may have implications for health, pharmacists need to be prepared to provide patient education on DTC genetic testing, particularly when the results may provide valuable insights into optimal pharmacotherapy. Pharmacists, regardless of practice site and specialty, are well positioned to provide patient education on the benefits, risks, and limitations of DTC pharmacogenomic tests in clinical practice and to advise patients about pursuing confirmatory clinical testing. This report highlights the responsibility of the pharmacist in the appropriate use of DTC genetic testing, particularly pharmacogenomic testing, using a patient case example across varied health settings.
Characterization of the foreign body response of titanium implants modified with polyphenolic coatings
Authors: Weber Florian , Quach Huy Quang , Reiersen Mathias , Sarraj Sadaf Yosef , Bakir Dyala Nidal , Jankowski Victor Aleksander , Nilsson Per H. , Tiainen Hanna 
Published: February 2022

AbstractThe foreign body response is dictating the outcome of wound healing around any implanted materials. Patients who suffer from chronic inflammatory diseases and impaired wound healing often face a higher risk for implant failure. Therefore, functional surfaces need to be developed to improve tissue integration. For this purpose, we evaluated the impact of surface coatings made of antioxidant polyphenolic molecules tannic acid (TA) and pyrogallol (PG) on the host response in human blood. Our results showed that although the polyphenolic surface modifications impact the initial blood protein adsorption compared to Ti, the complement and coagulation systems are triggered. Despite complement activation, monocytes and granulocytes remained inactivated, which was manifested in a low pro‐inflammatory cytokine expression. Under oxidative stress, both coatings were able to reduce intracellular reactive oxygen species in human gingival fibroblasts (hGFs). However, no anti‐inflammatory effects of polyphenolic coatings could be verified in hGFs stimulated with lipopolysaccharide and IL‐1β. Although polyphenols reportedly inhibit the NF‐κB signaling pathway, phosphorylation of NF‐κB p65 was observed. In conclusion, our results indicated that TA and PG coatings improved the hemocompatibility of titanium surfaces and have the potential to reduce oxidative stress during wound healing.
Amelioration of rotenone‐induced alterations in energy/redox system, stress response and cytoskeleton proteins by octanoic acid in zebrafish: A proteomic study
Authors: Sürmen Mustafa G. , Sürmen Saime , Cansız Derya , Ünal İsmail , Üstündağ Ünsal V. , Alturfan Ahmet A. , Büyükkayhan Derya , Emekli‐Alturfan Ebru 
Published: February 2022

AbstractRotenone is used to generate Parkinson's disease (PD)‐like symptoms in experimental animals. Octanoic acid (C8), is the principal fatty acid of medium‐chain triglycerides in ketogenic diets. Beneficial effects of ketogenic diets were shown in PD. We applied proteomic methods to reveal the effects of octanoic acid in rotenone toxicity in zebrafish to gain information on the use of ketogenic diets in PD. Zebrafish were exposed to 5 μg/ml rotenone and octanoic acid (20 and 60 mg/ml) for 30 days. LC‐MS/MS analysis was performed. Raw files were analyzed by Proteome Discoverer 2.4 software, peptide lists were searched against Danio rerio proteins. STRING database was used for protein annotations or interactions. 2317 unique proteins were quantified, 302 proteins were differentially expressed. Proteins involved in cell organization, biogenesis, transport, response to stimulus were most frequently expressed. Our study is first to report that the alterations in the expressions of proteins related to energy and redox system, stress response, and cytoskeleton proteins caused by rotenone exposure were normalized by octanoic acid treatment in zebrafish.
Are we choosing mobilization regimens for autologous stem cell transplantation in multiple myeloma wisely: A single center comparison of GCSF+/−plerixafor vs cyclophosphamide/GCSF+/−plerixafor
Authors: Yang Chloe , Dehghani Mina , Hopman Wilma , Bhella Sita 
Published: February 2022

AbstractBackgroundAutologous stem cell transplantation (ASCT) is a standard consolidation treatment for eligible patients with multiple myeloma (MM). There is no standardized mobilization regimen for collection of CD34+ stem cells, which is crucial to the success of ASCT. Cyclophosphamide/GCSF is an effective regimen, although reported associated toxicities include risk of febrile neutropenia (FN). Since plerixafor was introduced in Canada, this mobilization agent has been increasingly used as needed with GCSF at Kingston Health Science Centre (KHSC), with elimination of cyclophosphamide. This single center, retrospective, quality improvement study evaluates mobilization and ASCT outcomes of MM patients who had undergone stem cell mobilization at KHSC with cyclophosphamide/GCSF+/−plerixafor without antibiotics, cyclophosphamide/GCSF+/−plerixafor with antibiotics, and GCSF+/−plerixafor without antibiotics.MethodsA retrospective chart review was conducted evaluating 137 patients. The primary outcome measure was FN rates with mobilization. Balancing measures include CD34+ cell collected, plerixafor usage, days of apheresis and transplant outcomes. Chi‐square, ANOVA, or Kruskal‐Wallis methods were used to test statistical significance where appropriate.ResultsOur study noted a higher total and day one CD34+ count in the two groups utilizing cyclophosphamide in mobilization. All nine cases of FN occurred in these two groups (P < .05). Addition of antibiotics decreased, but did not eliminate risk of FN. There were no significant differences in the rate of plerixafor usage and number of apheresis days. Difference in transplant outcomes, including engraftment and transfusion support, were statistically but not clinically significant. A larger sample size may be needed to explore this fully. There was no significant difference in length of transplant hospital stay.ConclusionThe elimination of cyclophosphamide from mobilization regimens for MM appears to significantly reduce FN rates, without increasing balancing measures such as total number of apheresis days, plerixafor usage, duration of transplant hospitalization or mortality outcomes.
Deep generative models for ligand‐based de novo design applied to multi‐parametric optimization
Authors: Perron Quentin , Mirguet Olivier , Tajmouati Hamza , Skiredj Adam , Rojas Anne , Gohier Arnaud , Ducrot Pierre , Bourguignon Marie‐Pierre , Sansilvestri‐Morel Patricia , Do Huu Nicolas , Gellibert Françoise , Gaston‐Mathé Yann 
Published: February 2022

AbstractMulti‐parameter optimization (MPO) is a major challenge in new chemical entity (NCE) drug discovery. Recently, promising results were reported for deep learning generative models applied to de novo molecular design, but, to our knowledge, until now no report was made of the value of this new technology for addressing MPO in an actual drug discovery project. In this study, we demonstrate the benefit of applying AI technology in a real drug discovery project. We evaluate the potential of a ligand‐based de novo design technology using deep learning generative models to accelerate the obtention of lead compounds meeting 11 different biological activity objectives simultaneously. Using the initial dataset of the project, we built QSAR models for all the 11 objectives, with moderate to high performance (precision between 0.67 and 1.0 on an independent test set). Our DL‐based AI de novo design algorithm, combined with the QSAR models, generated 150 virtual compounds predicted as active on all objectives. Eleven were synthetized and tested. The AI‐designed compounds met 9.5 objectives on average (i.e., 86% success rate) versus 6.4 (i.e., 58% success rate) for the initial molecules measured on all objectives. One of the AI‐designed molecules was active on all 11 measured objectives, and two were active on 10 objectives while being in the error margin of the assay for the last one. The AI algorithm designed compounds with functional groups, which, although being rare or absent in the initial dataset, turned out to be highly beneficial for the MPO.
High expression of the ferroptosis‐associated MGST1 gene in relation to poor outcome and maladjusted immune cell infiltration in uterine corpus endometrial carcinoma
Authors: Yan Jianing , Ye Guoliang , Shao Yongfu 
Published: February 2022

AbstractBackgroundUterine corpus endometrial carcinoma (UCEC) tightly correlates with dysregulated iron homeostasis. MGST1 (microsomal glutathione S‐transferase 1) involves in the regulation of oxidative stress and plays a key role in inhibiting iron‐mediated cell death in cancer cells. Hence, we aimed to illuminate the characteristics of MGST1 expression and prognosis in UCEC using bioinformatics prediction to provide novel perspectives for theoretical supplementation and ferroptosis‐based immunotherapy.MethodsWe retrieved MGST1 expression data via several public data portals. The relationships between MGST1 expression and clinicopathologic characteristics as well as survival time were evaluated via multivariate methods and Kaplan–Meier survival curves. The MGST1‐interacting protein–protein interaction was also established by the STRING website. The TIMER and GEPIA databases were used to illustrate the association between MGST1 expression and infiltrated immune cells. We used the MethSurv website and the UALCAN website to determine the relationship between MGST1 expression and DNA methylation.ResultsMGST1 overexpression in UCEC compared with normal tissues correlates with different histological types, a lack of hormone therapy and poor survival time. MGST1 interacts with several ferroptosis‐related proteins. Overexpression of MGST1 was accompanied by lower levels of NK cell and CD8+ T cell infiltration, higher myeloid‐derived suppressor cell infiltration and different immunocytes with corresponding markers. Hypermethylation and low promoter methylation cooperate to regulate MGST1 expression.ConclusionElevated MGST1 expression is related to tumour development and poor prognosis, as well as dysregulated infiltration of immune cells in UCEC, which can be a potential prognostic indicator and ferroptosis‐based immunotherapy target.
A study on factors leading to poor mental health of children in Punjab, Pakistan
Authors: Huda Shamas Ul , Ahmad Liaquat , Rasul Sajid , Aslam Muhammad 
Published: February 2022

AbstractIn this article, the several factors have been investigated relating to the poor mental health of the children in Punjab, Pakistan. Using the data of the survey conducted by the Punjab Bureau of Statistics, different determinants of child malnutrition and poverty were diagnosed based upon sufficient information of the children under 5 year of age. Logistic regression analysis methodology has been applied for screening the most influential factors of the study. It has been observed that the rise in poverty has prejudiced the nutritional position of the children, the mother's education, and the fitness of the youth. It has also been observed that male children are more at risk to poor mental health than female children. A number of policy issues came to light from the findings of the study. The wealth index of all three anthropometric measures, the child malnutrition status of uneducated mothers, and the child diarrhea menace are the significant areas.
Pulmonary artery dopplers for early prediction of risk for bronchopulmonary dysplasia in extremely low birth weight babies
Authors: Lalitha Renjini , Bitar Eyad , Hicks Matthew , Hyderi Abbas , Kumaran Kumar 
Published: February 2022

AbstractObjectiveThe early abnormal pulmonary vasoreactivity observed in babies at risk of Bronchopulmonary dysplasia (BPD) increases the pulmonary vascular resistance. This can be assessed non‐invasively using Time to Peak Velocity:Right Ventricular Ejection Time ratio (TPV:RVET) measured from pulmonary artery Doppler waveform obtained using echocardiogram. We postulate that screening for this early can predict BPD in this cohort. The objective of the study was to determine the utility of TPV:RVET in early prediction of BPD in Extremely Low Birth Weight (ELBW) babies born less than 1250grams Birth Weight.MethodsThis was a single‐center retrospective cohort study of ELBW babies born<29 weeks over 4 year period who had echocardiogram between 7‐21 days of life. TPV:RVET ratio was measured from pulmonary artery Doppler waveform obtained using echocardiogram. The main outcome was BPD at 36 weeks corrected gestation. The predictive ability of TPV:RVET (cut off 0.34) for subsequent development of BPD was analyzed using ROC.ResultsOf 589 ELBW<29 weeks, 207 babies were eligible. BPD was found in 60.4%. The TPV:RVET at 0.34 had sensitivity 76.8% (95%CI 68.4–83.9), specificity 85.4% (95%CI 75.8–92.2), positive predictive value 88.9% (95%CI 81.4–94.1), negative predictive value 70.7% (95%CI 60.7–79), and ROC area 0.811 (95%CI 0.757–0.864). Odds ratio of having BPD for TPV:RVET at 0.34 was 19.9 (95%CI 8.19–48.34) and increased by 1.07 (95%CI 1.05–1.09) with every additional days of mechanical ventilation. TPV:RVET ratio had 92.75% inter‐observer agreement with kappa 0.83.ConclusionTPV:RVET ratio is a good and reliable early screening tool for subsequent development of BPD in ELBW babies with substantial inter‐observer agreement.