bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of Impurities in New Promising Antioxidants Bis-[3-(3,5-di-tert-butyl-4-hydroxyphenyl)propyl]sulfide and Dodecyl(3,5-dimethyl-4-hydroxybenzyl)sulfide
Authors: Shinko T. G. , Terentyeva S. V. , Yagunov S. E. , Kandalintseva N. V. , Prosenko O. I. , Ivanovskaya Ye. A. , Pinko P. I. 
Published: February 2022

Introduction. Dodecyl(3,5-dimethyl-4-hydroxybenzyl)sulfide (T1) and bis-[3-(3,5-di-tert-butyl-4-hydroxyphenyl)propyl]sulfide (T2) are the collaborative development of Novosibirsk State Pedagogical University and Novosibirsk Research Institute of Antioxidants. It was revealed in several experiments and research works that these substances have antioxidant, anti-inflammatory, hepatoprotective, cytoprotective, haemorheological activities. These facts make the objects of study promising medicinal antioxidant drugs. Consequently it’s necessary for the future production quality control to have standards and analytical methods for substances analysis.Aim. Impurities methods development and validation for the new biologically active substances T1 and T2.Materials and methods. HPLC method with UV-detection on 278 nm was carried out for the determination of impurities in objects of study. HPLC analysis were performed on ZORBAX SB-C18 (5 μm, 150 × 4,6 mm) column with isocratic regimen and with use of the acetonitrile:water mixture (T1) or acetonitrile (T2) as a mobile phase.Results and discussion. It was find out, that T1 has two unidentified impurities with concentration not more than 0,1 % during the shelf life. The chromatogramm of T2 has a peak of by-product of synthesis T2 – bis-[3-(3,5-di-tert-butyl-4-hydroxyphenyl)propyl]disulfide (T2-DS). Biologically safety of T2-DS was demonstrated in the previous works and the concentration of T2-DS was established to be not more, than 2,5 %. Furthermore, there was the one more unidentified impurity with concentration less, than 0,1 % on the chromatogram of T2. The developed HPLC methods were validated on characteristics «specificity», «linearity», «precision», «limit of quantification», «accuracy», «range».Conclusion. Methods for the determination of impurities in T1 and T2 were validated on the listed parameters. All the results meet the acceptance criteria: peaks on the chromatogramms are clearly separated; the correlation coefficients (r) are not more, than 0,980; accuracy was proved by linearity parameters; the value of the relative standard deviation is less, than 5 %; the intermediate precision for the both methods was proved by Fisher’s criterion and Student’s t-test.
Recovery e saúde mental: uma revisão da literatura latinoamericana
Authors: Oliveira Walter Ferreira de , Renato Pinto de Aquino Paulo , Cidade Ana , Bessa Sara Letícia , Bess Eduarda 
Published: February 2022

A abordagem Recovery emergiu nos anos 1970 nos Estados Unidos a partir dos movimentos em defesa dos direitos dos usuários dos serviços de saúde mental, ex-pacientes ou sobreviventes da psiquiatria. A partir da década de 2000 recovery vem orientando serviços de saúde mental em diversos países e muitos acreditam que possa contribuir com os processos de reforma do modelo de atenção em saúde mental em todo o mundo. No campo acadêmico, um número de publicações vem analisando as formas diversas de aplicação da abordagem em níveis nacionais e internacional. O objetivo desta revisão integrativa, realizada em 2019, foi analisar como a abordagem recovery vem sendo tratada na literatura científica na América Latina. A análise gerou categorias temáticas desvelando os principais assuntos abordados nestas publicações, entre eles discussões sobre terminologia, conceito de recovery, práticas, políticas e serviços orientados por recovery e possibilidades da incorporação da abordagem no contexto da Reforma Psiquiátrica Brasileira que, apesar dos obstáculos, ainda pode ser considerada como orientadora da Política Nacional de Saúde Mental no Brasil. A revisão aponta que o número de publicações sobre recovery na América Latina é escasso, comparado com o número de publicações nos Estados Unidos, Canadá, Hong Kong, Europa e Oceania, e são oriundas principalmente do Brasil. Percebe-se, ainda, que os autores brasileiros, em geral, entendem que recovery pode representar uma importante contribuição ao avanço da Reforma Psiquiátrica Brasileira e alguns defendem a necessidade de cautela sobre a incorporação da abordagem sem uma devida adaptação ao contexto social, cultural e econômico local. Também foi apontado que recovery tem sido aplicado em outros contextos sociais.
Recent Progress of Exosome Isolation and Peptide Recognition-Guided Strategies for Exosome Research
Authors: Xu Kun , Jin Yulong , Li Yongming , Huang Yanyan , Zhao Rui 
Published: February 2022

Exosomes are membrane extracellular vesicles secreted by almost all kinds of cells, which are rich in proteins, lipids, and nucleic acids. As a medium of intercellular communication, exosomes play important roles in biological processes and are closely related to the occurrence, and development of many diseases. The isolation of exosomes and downstream analyses can provide important information to the accurate diagnosis and treatment of diseases. However, exosomes are various in a size range from 30 to 200 nm and exist in complex bio-systems, which provide significant challenges for the isolation and enrichment of exosomes. Different methods have been developed to isolate exosomes, such as the “gold-standard” ultracentrifugation, size-exclusion chromatography, and polymer precipitation. In order to improve the selectivity of isolation, affinity capture strategies based on molecular recognition are becoming attractive. In this review, we introduced the main strategies for exosome isolation and enrichment, and compared their strengths and limitations. Furthermore, combined with the excellent performance of targeted peptides, we summarized the application of peptide recognition in exosome isolation and engineering modification.
Pericardial Involvement in ST-Segment Elevation Myocardial Infarction as Detected by Cardiac MRI
Authors: Massalha Eias , Brodov Yafim , Oren Daniel , Fardman Alex , Natanzon Sharon Shalom , Mazin Israel , Beinart Roy , Goldkorn Ronen , Konen Eli , Segni Elio Di , Segev Amit , Beigel Roy , Matetzky Shlomi , Goitein Orly 
Published: February 2022

BackgroundPost myocardial infarction pericarditis is considered relatively rare in the current reperfusion era. The true incidence of pericardial involvement may be underestimated since the diagnosis is usually based on clinical and echocardiographic parameters.ObjectivesThis study aims to document the incidence, extent, and prognostic implication of pericardial involvement in ST-segment elevation myocardial infarction (PISTEMI) using cardiac MRI (CMR).MethodsOne hundred and eighty-seven consecutive ST-segment elevation myocardial infarction patients underwent CMR on day 5 ± 1 following admission, including steady-state free precession (SSFP) and late Gadolinium enhancement (LGE) sequences. Late Gadolinium enhancement and microvascular obstruction (MVO) were quantified as a percentage of left ventricular (LV) mass. Late Gadolinium enhancement was graded for transmurality according to the 17 AHA left ventricle (LV) segment model (LGE score). Late pericardial enhancement (LPE), the CMR evidence of pericardial involvement, was defined as enhanced pericardium in the LGE series and was retrospectively recorded as present or absent according to the 17 AHA segments. Late pericardial enhancement was evaluated adjacent to the LV, the right ventricle, and both atria. Clinical, laboratory, angiographic, and echocardiographic data were collected. Clinical follow-up for major adverse cardiac events (MACE) was documented and correlated with CMR indices, including LGE, MVO, and LPE.ResultsLate pericardial enhancement (LPE+) was documented in 77.5% of the study cohort. A strong association was found between LPE and the degree and extent of myocardial injury (LGE, MVO). Both LGE and MVO were significantly correlated with increased MACE on follow-up. On the contrary, LPE presence, either adjacent to the LV or the other cardiac chambers, was associated with a lower MACE rate in a median of 3 years of follow-up HR 0.39, 95% CI (0.21–0.7), p = 0.002, and HR 0.48, 95% CI (0.26–0.9), p = 0.02, respectively.ConclusionsPrognostic implication of pericardial involvement in ST-segment elevation myocardial infarction was documented by CMR in 77.5% of our STEMI cohort. Late pericardial enhancement presence correlated significantly with the extent and severity of the myocardial damage. Unexpectedly, it was associated with a considerably lower MACE rate in the follow-up period.
Blood Bacterial 16S rRNA Gene Alterations in Women With Polycystic Ovary Syndrome
Authors: Wang Qing , Wang Qi , Zhao Lanbo , Bin Yadi , Wang Li , Wang Lei , Zhang Kailu , Li Qiling 
Published: February 2022

BackgroundEvidence proved the association between gut microbiome dysbiosis and polycystic ovary syndrome (PCOS) in metabolic disorder, decreased fertility, and hyperandrogenism. However, alterations in blood microbiome of PCOS remained unknown.ObjectiveThis study aims to measure the blood microbiome profile of PCOS patients compared with healthy controls by 16S rRNA sequencing and to investigate its association with PCOS.MethodsIn this case–control study, bacterial DNA in blood of 24 PCOS patients and 24 healthy controls was investigated by 16S rRNA gene sequencing using the MiSeq technology. Alpha and beta diversity were used to analyze within-sample biodiversity and similarity of one group to another, respectively. Linear discriminant analysis effect size (LEfSe) was calculated to determine biomarkers between groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional prediction was performed at genera level.ResultAlpha diversity of blood microbiome decreased significantly in women with PCOS, and beta diversity analysis demonstrated a major separation between the two groups. In the PCOS group, the relative abundance of Proteobacteria, Firmicutes, and Bacteroidetes decreased significantly, while Actinobacteria increased significantly. Cladogram demonstrated the microbiome differences between the two groups at various phylogenic levels. Meanwhile, linear discriminant analysis (LDA) presented significant decreases in Burkholderiaceae, Lachnospiraceae, Bacteroidaceae, Ruminococcaceae, and S24-7 and significant increases in Nocardioidaceae and Oxalobacteraceae of the PCOS group. KEGG pathway analysis at genera level suggested that 14 pathways had significant differences between the two groups.ConclusionOur findings demonstrated that blood microbiome had a significantly lower alpha diversity, different beta diversity, and significant taxonomic variations in PCOS patients compared with healthy controls.
Triglyceride to High-Density Lipoprotein Cholesterol (TG/HDL-C) Ratio, a Simple but Effective Indicator in Predicting Type 2 Diabetes Mellitus in Older Adults
Authors: Liu Hongzhou , Liu Jing , Liu Jixiang , Xin Shuanli , Lyu Zhaohui , Fu Xiaomin 
Published: February 2022

BackgroundA simple and readily available biomarker can provide an effective approach for the surveillance of type 2 diabetes mellitus (T2DM) in the elderly. In this research, we aim to evaluate the role of triglyceride to high-density lipoprotein cholesterol (TG/HDL-C) ratio as an indicator for new-onset T2DM in an elderly Chinese population aged over 75 years.MethodsThis longitudinal retrospective cohort study was conducted using a free database from a health check screening project in China. Participants with baseline TG and HDL measurements were enrolled, and the data of T2DM development were collected. The cumulative incident T2DM rates in different quintile groups of TG/HDL-C ratio (Q1 to Q5) were calculated and plotted. The independent effect of baseline TG/HDL-C ratio on T2DM risk during the follow-up period was tested by the Cox proportional hazard model. Subgroup analysis was also conducted to clarify the role of TG/HDL-C ratio in specific populations.ResultsA total of 231 individuals developed T2DM among 2,571 subjects aged over 75 years during follow-up. Regardless of adjustment for potential confounding variables, elevated TG/HDL-C ratio independently indicated a higher risk of incident T2DM [hazard ratio (HR) = 1.29; 95% confidence interval (CI), 1.14–1.47; P &lt; 0.01. As compared with the lowest quintile (Q1), elevated TG/HDL-C ratio quintiles (Q2 to Q5) were associated with larger HR estimates of incident T2DM [HR (95% CI), 1.35 (0.85–2.17), 1.31 (0.83–2.06), 1.85 (1.20–2.85), and 2.10 (1.38–3.20), respectively]. In addition, a non-linear correlation was found between TG/HDL-C ratio and the risk of T2DM, and the slope of the curve decreased after the cutoff point of 2.54. Subgroup analysis revealed a stronger positive correlation among male individuals and those with body mass index &lt;24 kg/m2.ConclusionsIncreased TG/HDL-C ratio indicates a greater risk of new-onset T2DM regardless of confounding variables. TG/HDL-C ratio is a simple but effective indicator in predicting T2DM in older adults. More future investigations are warranted to further promote the clinical application of TG/HDL-C ratio.
Complex Chromosomal Rearrangement Causes Male Azoospermia: A Case Report and Literature Review
Authors: Liang Yi , Xie Yingjun , Kong Shu , Pan Qianying , Qiu Wenjun , Wang Ding , Li Mengting , Lin Sisi , Liu Zihang , Sun Xiaofang 
Published: February 2022

Background: Male carriers of complex chromosomal rearrangements (CCRs) may have decreased fertility and usually present with azoospermia, oligospermia or teratospermia.Methods: High-resolution karyotype analysis using G-banding on peripheral blood lymphocytes was performed in an azoospermic male. Copy number variations (CNVs) were detected by chromosomal microarray analysis, and genetic variations were determined by long-read nanopore sequencing with Sanger sequencing for breakpoint confirmation.Results: The karyotype of the patient was 46,XY,t(4;21)(p11;p11),t(5;6;14)(p13q22;p22q22;q22), which did not involve CNVs with clinical significance. Twelve breakpoints in chromosomes 5, 6, and 14 were found by long-read nanopore sequencing. Reports on 17 males carrying CCRs with azoospermia were also reviewed.Conclusion: The extent of asynaptic regions in synaptonemal complexes during pachytene and the disruption of genes involved in male gametogenesis may cause azoospermia in CCR carriers.
Generation and Analysis of Pyroptosis-Based and Immune-Based Signatures for Kidney Renal Clear Cell Carcinoma Patients, and Cell Experiment
Authors: Lin Gaoteng , Feng Qingfu , Zhan Fangfang , Yang Fan , Niu Yuanjie , Li Gang 
Published: February 2022

Background: Pyroptosis is a programmed cell death caused by inflammasomes, which is closely related to immune responses and tumor progression. The present study aimed to construct dual prognostic indices based on pyroptosis-associated and immune-associated genes and to investigate the impact of the biological signatures of these genes on Kidney Renal Clear Cell Carcinoma (KIRC).Materials and Methods: All the KIRC samples from the Cancer Genome Atlas (TCGA) were randomly and equally divided into the training and testing datasets. Cox and Least Absolute Shrinkage and Selection Operator (LASSO) regression analysis were used to screen crucial pyroptosis-associated genes (PAGs), and a pyroptosis-associated genes prognostic index (PAGsPI) was constructed. Immune-associated genes (IAGs) related to PAGs were identified, and then screened through Cox and LASSO regression analyses, and an immune-associated genes prognostic index (IAGsPI) was developed. These two prognostic indices were verified by using the testing and the Gene Expression Omnibus (GEO) datasets and an independent cohort. The patients’ response to immunotherapy was analyzed. A nomogram was constructed and calibrated. qRT-PCR was used to detect the expression of PAGs and IAGs in the tumor tissues and normal tissues. Functional experiment was carried out.Results: 86 PAGs and 1,774 differentially expressed genes (DEGs) were obtained. After intersecting PAGs with DEGs, 22 differentially expressed PAGs (DEPAGs) were included in Cox and LASSO regression analyses, identifying 5 crucial PAGs. The PAGsPI was generated. Patients in the high-PAGsPI group had a poor prognosis. 82 differentially expressed IAGs (DEIAGs) were highly correlated with DEPAGs. 7 key IAGs were screened out, and an IAGsPI was generated. Patients in the high-IAGsPI group had a poor prognosis. PAGsPI and IAGsPI were verified to be robust and reliable. The results revealed patients in low-PAGsPI group and high-IAGsPI group may be more sensitive to immunotherapy. The calibrated nomogram was proved to be reliable. An independent cohort study also proved that PAGsPI and IAGsPI performed well in prognosis prediction. We found that the expression of AIM2 may affect proliferation of KIRC cells.Conclusion: PAGsPI and IAGsPI could be regarded as potential biomarkers for predicting the prognosis of patients with KIRC.
The Effect of Integration of Family Planning Into HIV Services on Contraceptive Use Among Women Accessing HIV Services in Low and Middle-Income Countries: A Systematic Review
Authors: Grant-Maidment Tallulah , Kranzer Katharina , Ferrand Rashida A. 
Published: February 2022

There is substantial unmet need for family planning (FP) among women living with HIV (WLHIV), leading to unintended pregnancies and may contribute indirectly to increasing the risk of transmission of HIV. This review aims to determine whether integration of FP into HIV testing and care results in increased use of contraception, a reduction in unmet need for FP, improved use of safer conception methods and a reduction in unintended pregnancies in low and middle-income countries. A systematic review was undertaken incorporating studies from PubMed, EMBASE, CINAHL, Web of Science and Global Health, the International AIDS Society Abstract Archive, the World STI &amp; HIV Congress Abstract Archive and the Conference on Retroviruses and Opportunistic Infections Abstract Archive published between 2016 and 2021, updating previous systematic reviews. After screening, 13 studies were included, 11 conducted in sub-Saharan Africa and 2 in India. The primary outcome of the review was contraceptive uptake and secondary outcomes included unmet need for FP, safer conception and unintended pregnancy. Integrated FP-HIV facilities were found to increase dual contraceptive use by at least 8% in five studies and modern contraceptive use by at least 8% in four studies. Findings from two studies suggested integration decreased the unmet need for contraception. Limited data prevented a conclusion from being drawn regarding whether integration increases safer conception. There was no evidence of integration reducing unintended pregnancies. The median quality score of studies was 3/9. Overall, integrated facilities have the potential of improving reproductive health of women accessing HIV services in LMICs. FP may be enhanced by including a safer conception component for WLHIV.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021251008, identifier: CRD42021251008.
Mortality Among Hospitalized Patients With Pleural Effusions. A Multicenter, Observational, Prospective Study
Authors: Markatis Eleftherios , Perlepe Garifallia , Afthinos Andreas , Pagkratis Konstantinos , Varsamas Charalampos , Chaini Eleftheria , Papanikolaou Ilias C. , Gourgoulianis Konstantinos I. 
Published: February 2022

BackgroundData regarding the prognostic significance of pleural effusion (PE) are scarce.ObjectiveExplore the impact of PE on mortality among hospitalized patients.MethodsMulticenter prospective observational study. Patients that underwent computed tomography (thorax and/or abdomen) and in which PE was detected, were admitted to the study. PE was classified by size on CT, anatomical distribution, diagnosis, and Light's criteria. Charlson comorbidity index (CCI), APACHE II, and SOFA score were calculated. Mortality at 1 month and 1 year were recorded.ResultsFive hundred and eight subjects, mean age 78 years. Overall mortality was 22.6% at 1 month and 49.4% at 1 year. Bilateral effusions were associated with higher mortality than unilateral effusions at 1 month (32 vs. 13.3%, p = 0.005) and large effusions with higher mortality than small effusions at 1 year (66.6 vs. 43.3%, p &lt; 0.01). On multivariate analysis age, CCI, APACHE II, SOFA score, and bilateral distribution were associated with short-term mortality, while long-term significant predictors were CCI, APACHE II, SOFA, and malignant etiology. Exudates (excluding MPE) exhibited a survival benefit at both 1 month and 1 year but due to the smaller sample, fluid characteristics were not included in the multivariate analysis.ConclusionsPleural effusion is a marker of advanced disease. Mortality is higher within the first month in patients with PEs related to organ failure, while patients with MPE have the worst long-term outcome. Independent predictors of mortality, apart from CCI, APACHE II, and SOFA scores, are age and bilateral distribution in the short-term, and malignancy in the long-term.
High-Density, Actively Multiplexed µECoG Array on Reinforced Silicone Substrate
Authors: Rachinskiy Iakov , Wong Liane , Chiang Chia-Han , Wang Charles , Trumpis Michael , Ogren John I. , Hu Zhe , McLaughlin Bryan , Viventi Jonathan 
Published: February 2022

Simultaneous interrogation of electrical signals from wide areas of the brain is vital for neuroscience research and can aid in understanding the mechanisms of brain function and treatments for neurological disorders. There emerges a demand for development of devices with highly conformal interfaces that can span large cortical regions, have sufficient spatial resolution, and chronic recording capability while keeping a small implantation footprint. In this work, we have designed 61 channel and 48 channel high-density, cortical, micro-electrocorticographic electrode arrays with 400 µm pitch on an ultra-soft but durable substrate. We have also developed a custom multiplexing integrated circuit (IC), methods for packaging the IC in a water-tight liquid crystal polymer casing, and a micro-bonding method for attaching the electronics package to the electrode array. With the integrated multiplexer, the number of external wire connections can be reduced to 16 wires, thereby diminishing the invasive footprint of the device. Both the electrode array and IC were tested in vivo in a rat model to demonstrate the ability to sense finely-localized electrophysiological signals.