bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation of Cryptococcus Laurentii Associated with Bovine Mastitis Cases in India
Authors: Chhabra R. , Shrinet G. , Yadav R. , Talukdar J. S. , Kakker N. K. , Goel P. 
Published: February 2022

Abstract
Mycotic mastitis mainly caused by yeasts like Cryptococcus spp. and Candida spp. have been emerging since last decade due to several factors like indiscriminate use of antibiotics, immune suppression, corticosteroid therapy, teat injuries and faulty milking machineries. The fungus, Cryptococcus laurentii is a non-neoformans, encapsulated, basidiomycete, which was earlier considered to be saprophytic and non-pathogenic. Now it is increasingly being reported in humans, especially in the immune compromised patients. In this study, Cryptococcus laurentii was isolated and identified from bovine mastitic milk samples. This probably might be the first report of isolation and identification of Cryptococcus laurentii from mastitic milk sample of buffalo from India to best of our knowledge. On milk culture examination, typical creamy white color colonies were appeared on Sabouraud's Dextrose agar, which on Gram’s staining gave budding yeast cells appearance. India ink staining revealed bright halo of capsules surrounding the yeast cells. All the isolates were positive for urease production and biofilm formation. Further confirmation was done using VITEK 2 compact system (BioMerieux) which was based on their biochemical tests profiles. Molecular confirmation was done by the PCR assay. Isolation and identification of this rare fungus from milk samples in present study raises a potential threat of zoonosis.
Role of cleaners in establishing and maintaining essential environmental conditions in healthcare facilities in Malawi
Authors: Elling Hayley , Behnke Nikki , Tseka Jennifer Mmodzi , Kafanikhale Holystone , Mofolo Innocent , Hoffman Irving , Reuland Frances , McCord Ryan , Cronk Ryan 
Published: February 2022

Abstract
Healthcare facility (HCF) cleaners play an important role in preventing healthcare-acquired infections and promoting a safe and clean healthcare environment. There is little evidence describing cleaner roles in HCFs in low-income countries and factors facilitating and constraining their roles and responsibilities. We analyzed qualitative and quantitative data from 57 in-depth interviews with cleaners in 44 government-run HCFs in Malawi. Cleaner constraints included inadequate training on infection prevention and control (IPC), personal protective equipment (PPE), hand hygiene, and waste management; insufficient PPE and hand hygiene resources; assignment of tasks that are unrelated to core responsibilities and their job description; risk of work-related injuries; and disrespect and stigma from medical staff, patients, and guardians. Facilitators included the positive collaboration and communication with medical staff, high job satisfaction, and a positive working attitude. We recommend the provision of more robust IPC, PPE, hand hygiene, and waste management training (including orientation and refresher). PPE resources must be made available to ensure HCF cleanliness and to keep cleaners, staff, patients, and guardians safe. Clearly defined job descriptions for cleaners will clarify their roles, describe bounds of their work, and ensure cleaners are viewed as essential frontline workers who ensure the safety of staff and patients.
An improved primary wastewater treatment system for a slaughterhouse industry: a full-scale experience
Authors: Shende Akshay D. , Dhenkula Swati , Rao Neti Nageswara , Pophali Girish R. 
Published: February 2022

Abstract
The effluent streams from individual slaughtering operations were segregated based on the degree of similarity and were treated separately. The wastewater from lairage and paunch sections was dominant in suspended solids (SS: 6,000–25,000 mg/L) and was separated using a hydrasieve (500 μm) and externally fed rotary drum filter (EFRDF, 200 μm), respectively. The SS removal efficiency of the hydrasieve and EFRDF was 75% and 55%, respectively, and remaining solids were removed through a primary clarifier. The fats, oils and grease (FOG: 12,000–35,000 mg/L) containing streams from the hide fleshing, rendering, intestine, and tripe washing were routed through a skimming tank. The SS and FOG removal efficiencies through the skimming tank were 75% and 90%, respectively. Any FOG remaining after the skimming tank was removed using dissolved air flotation which achieved 95% FOG removal. In addition, the efficiency of chemical oxygen demand removal through the primary treatment system was more than 80%. The effluent obtained after primary treatment was SS and FOG ≤ 200 and 100 mg/L. The segregation of streams and their separate treatment offered benefits such as resource recovery, reduced waste load on downstream secondary treatment and overall ease in slaughterhouse wastewater treatment.
Evaluation of the Antioxidant Potential of Natural Products
Authors: Lee Sang Kook , Mbwambo Zakaria H. , Chung HaSook , Luyengi Lumonadio , Gamez Esperenza J.C. , Mehta Rajendra G. , Kinghorn A. Douglas , Pezzuto John M. 
Published: February 2022

:
ince reactive oxygen radicals play an important role in carcinogenesis and other human disease states, antioxidants present in consumable fruits, vegetables, and beverages have received considerable attention as cancer chemopreventive agents. Thus, in order to identify antioxidants in plant extracts, test materials were assessed for potential to scavenge stable 1,2-diphenyl-2-picrylhydrazyl (DPPH) free radicals, reduce TPA-induced free radical formation in cultured HL-60 human leukemia cells, and inhibit responses observed with a xanthine/xanthine oxidase assay system. Approximately 700 plant extracts were evaluated, and 28 were found to be active in the DPPH free radical scavenging assay. Based on secondary analyses performed to assess inhibition of 7,12-dimethylbenz(a)anthracene-induced preneoplastic lesion formation with a mouse mammary organ culture model, Chorizanthe diffusa Benth. (Polygonaceae), Mezoneuron cucullatum Roxb. (Leguminosae), Cerbera manghas L. (Apocynaceae) and Daphniphyllum calycinum Benth. (Daphniphyllaceae) were selected and subjected to bioassay-guided fractionation. 5,7,3',5'-Tetrahydroxy-8,4'-dimethoxyflavonol, 5,8,4'-trihydroxy-7,3'-dimethoxyflavonol, 5,3',4'-trihydroxy-7-methoxyflavonol, and 6,3',4'-trihydroxy-7-methoxyflavonol were identified as active principles from C. diffusa. Piceatannol, trans-resveratrol, apigenin and scirpusin A were found as the active principles of M. cucullatum, olivil, (-)-carinol, and (+)-cycloolivil were active principles from C. manghas, and 5,6,7,4'-tetrahydroxyflavone 3-O-rutinoside and kaempferol 3-O-neohesperidoside were active principles from D. calycinum. Of these substances, the hydroxystilbenes piceatannol and transresveratrol have thus far been shown to inhibit carcinogen-induced preneoplastic lesion formation in the mouse mammary gland organ culture model.
Molecular Study of Klebsiella Oxytoca Associated with Urinary Tract Infection in Children
Authors: Shrief Raghdaa , Hassan Rasha H. , Zaki Maysaa El Sayed , Rizk Mohamed Anies 
Published: February 2022

Background:There are scarce reports about the association ofKlebsiella oxytoca(K. oxytoca) with urinary tract infection (UTI) in children. We aimed to evaluate the prevalence offimA, mrkA, matBandpilQadhesins genes and extended-spectrum beta-lactamase (ESBL) genesblaCTX-M, blaTEMandblaSHVby polymerase chain reaction (PCR) and to study biofilm formation and antibiotics resistance inK. oxytocafrom children with UTI.Methods:This study was a retrospective cross-sectional study that included 120 children with UTI due toK. oxytoca. The bacteria were subjected to molecular detection offimA, mrkA, matBandpilQadhesins genes and ESBL genesblaCTX-M, blaTEMandblaSHVby PCR. Biofilm capacity was determined by the microtiter plate method.Results:The isolatedK. oxytocahad positive ESBL activity in 45.8% of isolates. About 40% of isolates were biofilm producers. The frequency of adhesion genes amongK. oxytocawas 91.7%, 83.3%, 48.3% and 37.5% formatB, pilQ, fimAandmrkAgenes, respectively. For ESBL genes, the frequency was 38.3%, 36.7% and 33.3% forblaCTX-M, blaSHVandblaTEMgenes, respectively. The commonest genes among ESBL isolates wereblaCTX-M(83.6%),blaSHV(80%) thenblaTEMgene (72.7%). A significant association (p=0.048) was detected between ESBL activity and biofilm formation byK. oxytoca.Conclusion:Present study highlights the emergence ofK. oxytocaas a pathogen associated with UTI in children. There was a high prevalence of adhesin genes and ESBL genes among these isolates. The capacity ofK. oxytocato form biofilm was associated with ESBL production.
The Effects of mHealth-Based Gamification Interventions on Participation in Physical Activity: Systematic Review
Authors: Xu Linqi , Shi Hongyu , Shen Meidi , Ni Yuanyuan , Zhang Xin , Pang Yue , Yu Tianzhuo , Lian Xiaoqian , Yu Tianyue , Yang Xige , Li Feng 
Published: February 2022

BackgroundIt is well known that regular physical exercise has associated benefits; yet, participation remains suboptimal. Mobile health (mHealth) has become an indispensable medium to deliver behavior change interventions, and there is a growing interest in the gamification apps in mHealth to promote physical activity (PA) participation. Gamification could use game design elements (such as points, leaderboards, and progress bars), and it has the potential to increase motivation for PA and engagement. However, mHealth-based gamification interventions are still emerging, and little is known about the application status and efficacy of such interventions.ObjectiveThis systematic review aims to investigate gamification apps in mHealth for improving PA levels and simultaneously summarize the impact of gamification interventions on PA participation.MethodsWe searched PubMed, Scopus, Web of Science, Embase, CINAHL (EBSCO host), and IEEE Xplore from inception to December 20, 2020. Original empirical research exploring the effects of gamification interventions on PA participation was included. The papers described at least one outcome regarding exercise or PA participation, which could be subjective self-report or objective indicator measurement. Of note, we excluded studies about serious games or full-fledged games.ResultsOf 2944 studies identified from the database search, 50 (1.69%) were included, and the information was synthesized. The review revealed that gamification of PA had been applied to various population groups and broadly distributed among young people but less distributed among older adults and patients with a disease. Most of the studies (30/50, 60%) combined gamification with wearable devices to improve PA behavior change, and 50% (25/50) of the studies used theories or principles for designing gamified PA interventions. The most frequently used game elements were goal-setting, followed by progress bars, rewards, points, and feedback. This review demonstrated that gamification interventions could increase PA participation; however, the results were mixed, and modest changes were attained, which could be attributed to the heterogeneity across studies.ConclusionsOverall, this study provides an overview of the existing empirical research in PA gamification interventions and provides evidence for the efficacy of gamification in enhancing PA participation. High-quality empirical studies are needed in the future to assess the efficacy of a combination of gamification and wearable activity devices to promote PA, and further exploration is needed to investigate the optimal implementation of these features of game elements and theories to enhance PA participation.
Digital Health Technologies for Long-term Self-management of Osteoporosis: Systematic Review and Meta-analysis
Authors: Alhussein Ghada , Hadjileontiadis Leontios 
Published: February 2022

Background
Osteoporosis is the fourth most common chronic disease worldwide. The adoption of preventative measures and effective self-management interventions can help improve bone health. Mobile health (mHealth) technologies can play a key role in the care and self-management of patients with osteoporosis.

Objective
This study presents a systematic review and meta-analysis of the currently available mHealth apps targeting osteoporosis self-management, aiming to determine the current status, gaps, and challenges that future research could address, as well as propose appropriate recommendations.

Methods
A systematic review of all English articles was conducted, in addition to a survey of all apps available in iOS and Android app stores as of May 2021. A comprehensive literature search (2010 to May 2021) of PubMed, Scopus, EBSCO, Web of Science, and IEEE Xplore was conducted. Articles were included if they described apps dedicated to or useful for osteoporosis (targeting self-management, nutrition, physical activity, and risk assessment) delivered on smartphone devices for adults aged ≥18 years. Of the 32 articles, a random effects meta-analysis was performed on 13 (41%) studies of randomized controlled trials, whereas the 19 (59%) remaining studies were only included in the narrative synthesis as they did not provide enough data.

Results
In total, 3906 unique articles were identified. Of these 3906 articles, 32 (0.81%) articles met the inclusion criteria and were reviewed in depth. The 32 studies comprised 14,235 participants, of whom, on average, 69.5% (n=9893) were female, with a mean age of 49.8 (SD 17.8) years. The app search identified 23 relevant apps for osteoporosis self-management. The meta-analysis revealed that mHealth-supported interventions resulted in a significant reduction in pain (Hedges g −1.09, 95% CI −1.68 to −0.45) and disability (Hedges g −0.77, 95% CI −1.59 to 0.05). The posttreatment effect of the digital intervention was significant for physical function (Hedges g 2.54, 95% CI −4.08 to 4.08) but nonsignificant for well-being (Hedges g 0.17, 95% CI −1.84 to 2.17), physical activity (Hedges g 0.09, 95% CI −0.59 to 0.50), anxiety (Hedges g −0.29, 95% CI −6.11 to 5.53), fatigue (Hedges g −0.34, 95% CI −5.84 to 5.16), calcium (Hedges g −0.05, 95% CI −0.59 to 0.50), vitamin D intake (Hedges g 0.10, 95% CI −4.05 to 4.26), and trabecular score (Hedges g 0.06, 95% CI −1.00 to 1.12).

Conclusions
Osteoporosis apps have the potential to support and improve the management of the disease and its symptoms; they also appear to be valuable tools for patients and health professionals. However, most of the apps that are currently available lack clinically validated evidence of their efficacy and focus on a limited number of symptoms. A more holistic and personalized approach within a cocreation design ecosystem is needed.

Trial Registration
PROSPERO 2021 CRD42021269399; https://tinyurl.com/2sw454a9
Perspectives From French and Filipino Parents on the Adaptation of Child Health Knowledge Translation Tools: Qualitative Exploration
Authors: Elliott Sarah A , Wright Kelsey S , Scott Shannon D , Hartling Lisa 
Published: February 2022

Background
A number of evidence-based knowledge translation (KT) tools for parents of children with acute health conditions have been developed. These tools were created and tested with parental input and disseminated to groups proficient in English. Therefore, it is unclear whether they are useful for populations that are more diverse. To enhance the reach of our current and future KT tools, language translation and cultural adaptations may promote relevance for previously underserved knowledge users.

Objective
This study aims to explore and understand considerations for the cultural and linguistic adaptation of a KT tool in French and Filipino communities.

Methods
A KT tool (whiteboard animation video) describing the signs and symptoms of croup was originally developed in English to provide parents with evidence-based information couched within a narrative reflecting parents’ experiences with the condition. This KT tool was adapted (linguistics and imagery) for French- and Tagalog-speaking parents and caregivers through feedback from key stakeholders. The videos were presented to the respective language speakers for usability testing and discussion. Participants were asked to view the KT tool, complete a usability survey, and participate in semistructured interviews. Audio recordings from the interviews were transcribed verbatim, translated into English, and analyzed for relevant themes by using thematic analysis.

Results
French- (n=13) and Tagalog-speaking (n=13) parents completed the usability survey and were interviewed. Although analyzed separately, both data sets produced similar findings, with key themes relating to understanding, relatability, and accessibility. Both the French and Tagalog groups reported that the video and other KT tools were useful in their adapted forms. Participants in both groups cautioned against using verbatim vocabulary and suggested that cultural competency and understanding of health languages were essential for high-quality translations. Parents also discussed their preference for videos with diverse visual representations of families, home environments, and health care workers, as such videos represent their communities more broadly.

Conclusions
French and Filipino parents appreciated having KT tools in their first language; however, they were also supportive of the use of English KT products. Their suggestions for improving the relatability and communication of health messages are important considerations for the development and adaptation of future KT products. Understanding the needs of the intended end users is a crucial first step in producing relevant tools for health evidence dissemination.
Adding an App-Based Intervention to the Cognitive Behavioral Analysis System of Psychotherapy in Routine Outpatient Psychotherapy Treatment: Proof-of-Concept Study
Authors: Netter Anna-Lena , Beintner Ina , Brakemeier Eva-Lotta 
Published: February 2022

Background
The Cognitive Behavioral Analysis System of Psychotherapy (CBASP) is an empirically supported psychotherapeutic treatment developed specifically for persistent depressive disorder. However, given the high rates of nonresponse and relapse, there is a need for optimization. Studies suggest that outcomes can be improved by increasing the treatment dose via, for example, the continuous web-based application of therapy strategies between sessions. The strong emphasis in CBASP on the therapeutic relationship, combined with limited therapeutic availabilities, encourages the addition of web-based interventions to face-to-face therapy in terms of blended therapy.

Objective
The aim of this study was to test an app-based intervention called CBASPath, which was designed to be used as a blended therapy tool. CBASPath offers 8 sequential modules with app-based exercises to facilitate additional engagement with the therapy content and a separate exercise to conduct situational analyses within the app at any time.

Methods
CBASPath was tested in an open pilot study as part of routine outpatient CBASP treatment. Participating patients were asked to report their use patterns and blended use (integrated use of the app as part of therapy sessions) at 3 assessment points over the 6-month test period and rate the usability and quality of and their satisfaction with CBASPath.

Results
The results of the pilot trial showed that 93% (12/13) of participants used CBASPath as a blended tool during their therapy and maintained this throughout the study period. Overall, they reported good usability and quality ratings along with high user satisfaction. All participants showed favorable engagement with CBASPath; however, the frequency of use differed widely among the participants and assessment points. Situational analysis was used by all participants, and the number of completed modules ranged from 1 to 7. All participants reported blended use, although the frequency of integration in the face-to-face sessions varied widely.

Conclusions
Our findings suggest that the digital augmentation of complex and highly interactive CBASP therapy in the form of blended therapy with CBASPath is feasible in routine outpatient care. Therapeutic guidance might contribute to high adherence and increase patient self-management. A few adjustments, such as saving entries directly in the app, could facilitate higher user engagement. A randomized controlled trial is now needed to investigate the efficacy and added value of this blended approach. In the long term, CBASPath could help optimize persistent depressive disorder treatment and reduce relapse by intensifying therapy and providing long-term patient support through the app.
Balance y perspectivas de los contenidos literarios en Decires
Authors: Cañedo César , Molano Nucamendi Horacio 
Published: February 2022

En el contexto de los preparativos para celebrar los cien años del CEPE como institución educativa es primordial realizar una valoración de Decires, su publicación periódica más importante. Como se expondrá en los siguientes párrafos, la revista ha cumplido con distintas misiones, además de ser, desde siempre, un vehículo de comunicación de sus logros académicos. Sus iniciativas parten de su cuerpo docente, por lo que sobresalen artículos de la autoría de profesores del Centro, con los pros y contras que esto supone.
El rumbo de Decires se orienta conforme lo ha decidido su equipo editorial, el cual se ha ido renovando desde 1998, cuando apareció el primer número. A continuación, se describe a detalle la naturaleza de las colaboraciones orientadas a la apreciación de las letras hispánicas en el horizonte de la labor de la enseñanza del español a estudiantes extranjeros.
A fin de facilitar este repaso de 24 números —incluyendo el extraordinario por los 90 años del CEPE— el artículo se dividió en las dos etapas significativas de la continuidad editorial. La primera es la edición en papel, que tuvo tres propuestas de diseño: una época fundacional, la de los números 1 al 4, luego un replanteamiento en los números 5 y 6, y una más en la que finalmente se estableció la división de los contenidos de la revista en cinco secciones (una por cada departamento del CEPE: Arte, Español, Historia y Literatura más el espacio de Reseñas) desde el número 7 hasta el 11. El soporte digital marca un segundo momento, e inició cuando se cumplieron los primeros diez años de Decires, a partir del número 12-13.