bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic interactions control the spread of plasmid-encoded functional novelty during microbial range expansion
Authors: Ma Yinyin , Kan Anton , Johnson David R. 
Published: June 2022

AbstractSurface-associated microbial communities are omnipresent on Earth. As individuals grow and divide within these communities, they undergo range expansion during which different cell-types arrange themselves across space to form spatial patterns (referred to as spatial self-organization). Metabolic interactions are important determinants of the spatial self-organization process, where they direct the spatial positionings of different cell-types. We hypothesized here a previously unexplored consequence of metabolic interactions; by directing the spatial positionings of different cell-types, they also control the horizontal spread of functional novelty during range expansion. We focused on a form of functional novelty of critical importance to human health – the conjugative transfer and proliferation of plasmid-encoded antibiotic resistance. We performed range expansion experiments and spatially-explicit individual-based computational simulations with pairs of strains of the bacterium Pseudomonas stutzeri, where one strain was a plasmid donor and the other a potential recipient. We then imposed a competitive or resource cross-feeding interaction between them. We found that interactions that increase the spatial intermixing of strains also increase plasmid conjugation. We further directly linked these effects to spatial intermixing itself. We finally showed that the ability of plasmid recipients to proliferate is determined by their spatial positionings. Our results demonstrate that metabolic interactions are indeed important determinants of the horizontal spread of functional novelty during microbial range expansion, and that the spatial positionings of different cell-types need to be considered when predicting the proliferation and fate of plasmid-encoded traits.
Programmable Mixed-Signal Biocomputers in Mammalian Cells
Authors: Letendre Justin H. , Weinberg Benjamin H. , Mendes Marisa , Marano Jeffery M. , Benman K. J. William , Petherbridge Rachel , Drezek Kamila , Koplik Samantha E. , Piñeiro Alexandra , Wong Wilson W. 
Published: June 2022

AbstractLiving cells perform sophisticated computations that guide them toward discrete states. Synthetic genetic circuits are powerful tools for programing these computations, where transcription-regulatory networks and DNA recombination are the two dominant paradigms for implementing these systems. While each strategy exhibits unique strengths and weaknesses, integrating both into one seamless design framework would enable advanced gene circuit designs intractable with either approach alone. Here, we present Computation via Recombinase Assisted Transcriptional Effectors (CREATE), which leverages site-specific recombination to perform robust logic on discreet computational layers and programmable transcription factors that connect these layers, allowing individual calculations to contribute toward larger operations. We demonstrate the functionality of CREATE by producing sophisticated circuits using a simple plug- and-play framework, including 189 2-input-3-output circuits, modular digital-to-analog signal converters, a 2-bit multiplier circuit, and a digital and analog mixed-signal generator. This work establishes CREATE as a versatile platform for programming complex signal processing systems capable of high-fidelity logic computation and tunable control over circuit output levels.One-Sentence SummaryWe present a minimal and robust genetic circuit platform for programming cells with sophisticated signal processing capabilities.
Pangenomic analysis reveals plant NAD+manipulation as an important virulence activity of bacterial pathogen effectors
Authors: Hulin Michelle T. , Hill Lionel , Jones Jonathan D. G. , Ma Wenbo 
Published: June 2022

AbstractNicotinamide adenine dinucleotide (NAD+) has emerged as a key component in prokaryotic and eukaryotic immune systems and the recent discovery that Toll/interleukin-1 receptor (TIR) proteins function as NAD+hydrolases (NADase) link NAD+-derived small molecules with immune signalling. We investigated pathogen manipulation of host NAD+metabolism as a virulence strategy. Using the pangenome of the model bacterial pathogenPseudomonas syringae,we conducted a structure-based similarity search from 35,000 orthogroups for type III effectors (T3Es) with potential NADase activity. Thirteen T3Es, including five newly identified candidates, were identified that possess domain(s) characteristic of seven NAD+-hydrolyzing enzyme families. MostP. syringaestrains that depend on the Type III secretion system to cause disease, encode at least one NAD+-manipulating T3E, and many have several. We experimentally confirmed the type III-dependent secretion of a novel T3E, named HopBY, which shows structural similarity to both TIR and adenosine diphosphate ribose (ADPR) cyclase. Homologs of HopBY were predicted to be type VI effectors in diverse bacterial species, indicating potential recruitment of this activity by microbial proteins secreted during various interspecies interactions. HopBY efficiently hydrolyzes NAD+and specifically produces 2’cADPR, which can also be produced by TIR immune receptors of plants and other bacteria. Intriguingly, this effector promoted bacterial virulence, indicating that 2’cADPR may not be the signalling molecule that directly initiates immunity. This study highlights a host-pathogen battle ground centred around NAD+metabolism and provides insight into the NAD+-derived molecules involved in plant immunity.Significance statementNAD+metabolism plays a crucial role in plant and bacterial immunity. However, the diversity and scope of NAD+processing steps in immune signalling remain unclear. Furthermore, whether pathogens can manipulate NAD+metabolism to promote virulence is unknown. By conducting a pangenomic screen of the plant pathogenP. syringae,we found 13 type III effectors that potentially possess NADase activities, indicating that NAD+manipulation is an important virulence mechanism. Further characterization of a newly identified effector HopBY showed that it produces a cyclic ADP-ribose isomer (2’cADPR) and promotes bacterial infection and symptom development. This study clarifies the role of 2’cADPR in immune signalling and provides an example of effectors as useful molecular probes to understand immunity.
Investigating the impacts of microlevel CSR activities on firm sustainability: mediating role of CSR performance and moderating role of top management support
Authors: Chatterjee Sheshadri , Chaudhuri Ranjan , Vrontis Demetris 
Published: June 2022

PurposeThe purpose of this study is to investigate the impacts of microlevel corporate social responsibility (CSR) activities on firm sustainability. The study also examines the mediating roles of CSR performance (CSRP) and top management support on firm sustainability.Design/methodology/approachWith the help of existing literature and theories, a theoretical model has been developed, which is then validated using structural equation modeling technique considering 327 respondents who perform CSR activities at respondents' firms in India. The research methods include moderator analysis to understand the role of top management support for firm sustainability.FindingsThis research found that microlevel CSR activities have a significant positive impact on CSR performance. The study also found that there is a significant moderating impact of top management support on the relationship between CSR performance and firm sustainability.Research limitations/implicationsThis study proposed a theoretical model which has established the relationships between the microlevel CSR activities and CSRP along with firm sustainability. This is an effective model and provides vital inputs to the firms on how to succeed with CSR activities. Limitations to this study's generalizability include use of cross-sectional data and that the data were collected from one country.Originality/valueThe proposed theoretical model is unique and can be applied by firms to enhance firms' CSR performance. There is no other study which has investigated the moderating role of firm leadership team impacting the relationship between CSR performance and firm sustainability. Thus, this study is a unique attempt and adds value to the extant literature on CSR as well as firm sustainability.
The acceptance and continued use of blockchain technology in supply chain management: a unified model from supply chain professional's stance
Authors: Shahzad Khuram , Zhang Qingyu , Khan Muhammad Kaleem , Ashfaq Muhammad , Hafeez Muhammad 
Published: June 2022

PurposeThis study pinpoints the critical factors influencing the acceptance of blockchain technology in supply chain management in the light of the extended unified theory of acceptance and use of technology (UTAUT2) with additional factors personal innovativeness in technology and user's self-efficacy.Design/methodology/approachThe questionnaire-based data was obtained from SC professionals in China (Beijing). The essential factors influencing it are evaluated through structural equation modeling (SEM), using AMOS software.FindingsThe empirical findings specify that performance expectancy, facilitating conditions, price value, hedonic motivation, user self-efficacy, and personal innovativeness are positively influencing user satisfaction. User satisfaction has a substantial progressive effect on habit. Furthermore, facilitating conditions, price value, habit, user self-efficacy, personal innovativeness, and user satisfaction have a progressive impact on continued intention to use blockchain technology in supply chain management.Originality/valueAlthough numerous studies investigated the influencing factors of blockchain technology adoption in supply chain management, no study examined the determinants of UTAUT2. However, this study not only empirically studied the UTAUT2 model but also extended it with the most influencing elements such as personal innovativeness in technology and user's self-efficacy. Furthermore, this study contributes to the BT-enabled SCM literature by studying the continued use and acceptance, rather than testing behavioral intention and initial adoption which is common in previous studies of BT-enabled SCM. Finally, this study discusses the limitations, future directions, and managerial implications of the results so that supply chain professionals can deliver what supply chain stakeholders require.
Student satisfaction with EMI courses: the role of motivation and engagement
Authors: Le Nguyen Thi , Nguyen Duong Tuan 
Published: June 2022

PurposeIn response to internationalization and globalization, especially in higher education, universities in non-English-speaking countries have implemented English as a medium of instruction (EMI). The purpose of this study is to assess the satisfaction of students in terms of dimensions of EMI courses and examine the relationship between student motivation, engagement, and satisfaction with EMI courses.Design/methodology/approachBy using a quantitative approach based on structured questionnaires of 437 Vietnamese undergraduate students, this study applied hierarchical regression analysis to examine the relationship between student motivation, engagement, and satisfaction with EMI courses.FindingsStudents have a relatively positive perception of the EMI courses that they have taken. In particular, they were most satisfied with teachers' teaching characteristics and least satisfied with students' learning characteristics. The study also confirmed that cognitive and emotional engagement have mediating effects on the relationship between motivation and students' satisfaction with EMI courses.Originality/valueThis study suggests that both educational institutions and teachers in non-speaking English countries should pay more attention to motivational factors to engage students in learning and ensure that they are satisfied with EMI courses.
Monetary policies and Islamic mutual fund flows: evidence from Islamic republic of Iran
Authors: AliAhmadi Saeid , Soroushyar Afsaneh 
Published: June 2022

Purpose
The main purpose of this study is to analyze the impact of monetary policies on Islamic mutual fund flows in the Islamic Republic of Iran.

Design/methodology/approach
In this study, a panel regression model was used to test the research hypotheses. The sample consists of 4,760 seasonality data and 119 Islamic mutual funds over ten-year period between 2011 and 2020. The dependent variable of the study is the cash flow of Islamic mutual funds and the independent variable of monetary policies includes the money growth rate, the liquidity growth rate, interest rate and inflation rate.

Findings
Based on the results of the hypothesis test, all research variables including money growth rate, liquidity growth rate, interest rate and inflation rate have a negative and significant impact on Islamic mutual fund flows. These findings are consistent with the efficient market hypothesis and signaling theory.

Research limitations/implications
This study has implication for policymakers, regulators and fund managers. The results show that the negative impact of monetary policies on Islamic mutual fund flows has a direct effect on the allocation decisions and investment strategies of Islamic mutual fund investors and managers. Also, the results of the research can reduce policymakers’ concerns about monetary policies and provide them forward-looking guidance policy.

Originality/value
To the best of the authors’ knowledge, this is the first study to empirically examine the impact of monetary policies on Islamic mutual fund flows in an emerging economy and provides a significant contribution to the literature of mutual funds.
Industry convergence and value innovation: a bibliometric analysis and systematic review
Authors: Chen Ye , Shen Lei , Zhang Xi , Chen Yutao 
Published: June 2022

PurposeThe purpose of this paper is twofold: first, to present a bibliometric analysis and systematic literature review of industry convergence and value innovation to understand the current research status; second, to provide a coherent theoretical research framework for future research.Design/methodology/approachThis study adopts a two-step analysis approach by combining bibliometric analysis and systematic literature review to explore the research topic of industry convergence and value innovation. Besides, two bibliometric tools, HistCite and VOSviewer, were applied to this study.FindingsThis study found that Stefanie Bröring and Fredrik Hacklin are the top two most influential authors among all authors in the sample publications. Technological Forecasting and Social Change is one of the top-ranking journal that often publishes this topic of articles. Germany and the University of Munster are the most influential country and institutions, respectively. Besides, five core research themes were identified based on keywords co-occurrence map, theoretical lenses, factors promoting industry convergence, indicators of industry convergence, the impact of industry convergence and emerging research directions. Based on the above analysis, this paper constructed a theoretical research framework of industry convergence and value innovation.Research limitations/implicationsThis paper only draw data from one database – Web of Science – which cannot provide broad coverage of the research topic. Besides, the bibliometric method of this paper is based on high local citation score and high-frequency words, articles in the skirting subjects’ area may not be analyzed.Practical implicationsWith the rapid development of technology, such as nanotechnology, radio - frequency identification (RFID), etc., the iterative upgrading of products also comes. As a result, the boundary between industries is gradually blurred, and the phenomenon of industry convergence appears. Therefore, managerial decision-makers are facing challenges of how to respond to the convergence phenomena. From the firm level, firms are facing the problem of value innovation of the existing product, new product development and core competence improvement. Industries are facing the problem of transformation and upgrading. This paper provides certain theoretical insights for both firms and industries to guide the practice accordingly.Originality/valueThis paper is the first to use a bibliometric method to examine the topic of industry convergence and value innovation. In addition, this paper presents an in-depth analysis of this topic and provides a comprehensive theoretical research framework for future study.
Research on the mechanism of knowledge coupling affecting firms' innovation results driven by digitization——based on system dynamics methodology
Authors: Huiping Zhou , Yuxin Yang 
Published: June 2022

PurposeThe purpose of this paper is to build a System Dynamics model to reveal the structure and dynamics of knowledge coupling affecting firms' innovation results in the digital context.Design/methodology/approachDrawing on the recombined view of innovation, this paper divides knowledge coupling into two dimensions: component knowledge coupling and architectural knowledge coupling. Then, the authors build a system dynamics model to identify the interaction of knowledge coupling factors and use the professional Vensim PLE to conduct simulation analysis to capture the dynamic interaction of motivation factors in knowledge coupling system.FindingsThe results show that both technology resources and digital dynamic capability play positive effects in the mechanism of knowledge coupling influencing firms' innovation results, while organizational inertia negatively affects the process of knowledge coupling to achieve innovation outcomes.Originality/valueThis study develops a holistic system dynamics model to reveal and elaborate on the complex dynamic mechanism of knowledge coupling impacting firms' innovation results in the context of digitization and provides a theoretical reference for companies to effectively adopt digital technology to carry out knowledge coupling strategy.
Reverse knowledge transfer: making sense of two decades of academic research
Authors: Singh Nivisha , Pandey Ritesh , Gupta Rohit , Biswas Baidyanath , Gupta Shubhi 
Published: June 2022

Purpose
This study aims to synthesize extant literature on the concept of reverse knowledge transfer (RKT) built up over the past two decades with the help of bibliometric analyssis and also to suggest promising new areas for research in this field.

Design/methodology/approach
The study adopts a literature review methodology combined with bibliometric and network analysis based on 117 papers identified from the Scopus database. In particular, this study has tried to identify and capture themes not previously fully captured or evaluated by other reviews on this topic.

Findings
The authors have identified research themes and research gaps in the area of RKT. Overall, the review shows the main outlets that have published papers on RKT and the theoretical background this research is built on. This study exhibits core themes in this area that have persisted and grown consistently such as the subsidiary’s role in RKT. In addition, the review highlights less researched themes such as role of boundary spanning in RKT, which open exciting avenues for new research opportunities.

Research limitations/implications
This study finds that RKT research has experienced remarkable growth from a complete viewpoint in recent years. There was a surge in publications in the area from 2008 onwards, and many of its influential papers seemed to have been published between 2013 and 2018. Prominent themes in this body of research have been identified and potential for future studies has been explored.

Originality/value
To the best of the authors’ knowledge, this is the first study to map, synthesize and discuss the literature concerning RKT.
Performance and design comparison of moving-magnet linear oscillating actuators based on their mover positions
Authors: Ahmad Afaq , Ahmad Zahoor , Ullah Abdullah , Rehman Naveed Ur Ur , Israr Muhammad , Zia Muhammad , Ali Haider , Rahman Ataur 
Published: June 2022

Purpose
This study aims to investigate and compare the characteristics of three topologies of moving-magnet linear oscillating actuator (LOA) based on their mover position. Positive aspects and consequences of every topology are demonstrated. Three topologies of axially magnetized moving-magnet LOA; outer mover, inner mover (IM) and dual stator (DS) are designed and examined. Due to its characteristically high thrust density and more mechanical strength, axially magnetized tubular permanent magnets (PMs) are used in these topologies.

Design/methodology/approach
LOAs are designed and optimized using parametric sweep, in term of design parameters and output parameters like thrust force, stroke and operating resonance frequency of the LOA. All the pros and cons of each topology are investigated and compared. Output parameters of the LOAs are compared using same size of the investigated LOAs. Mover mass, which plays a vital role in resonant operation, is analyzed for IM and DS designs. Investigated LOAs are compared with conventional designs of LOA for compressor in refrigeration system with regards of motor constant, stroke and thrust per PM mass.

Findings
This paper analyzes three topologies of moving-magnet LOAs. The basic difference between investigated LOAs is the radius of tubular-shaped mover from its central axis. All the design parameters are compared and concluded that thrust per PM mass of IMLOA is maximum. OMLOA provides maximum motor constant of value 180 N/A. DSLOA provides thrust force with motor constant 120 N/A and required intermediate materials of PMs. All the three designs give the best results in terms of motor constant and thrust per PM mass, compared to conventional designs of LOA.

Originality/value
This paper determines the impact of mover position from its central axis in a tubular-shaped moving-magnet LOA. This work is carried out in correspondence of latest papers of LOA.