bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality of life in intermittent exotropia for Korean children and their parents
Authors: Oh Jin Seon , Jung Jae Ho , Shin Hyun Jin 
Published: November 2022

Abstract
Background: Patients with strabismus are more likely to have mental health problems, including high rates of depressive symptoms and social phobia. Intermittent exotropia (IXT) typically occurs in early childhood and is more common in Asian populations. We aim to assess the health-related quality of life (HRQOL) concerns in children with intermittent exotropia (IXT) using the Intermittent Exotropia Questionaire (IXTQ), and their associations with the clinical severity of IXT and the parents’ HRQOL concerns.
Methods:One hundred twenty-two children with IXT (aged 5–17 years) and one parent for each child completed the child and parent IXTQ, respectively. The final IXTQ score is calculated using the mean score for all items, and ranges from 0 (worst HRQOL) to 100 (best HRQOL). The correlations of child IXTQ scores with their deviation angle and stereoacuity were measured, as were those with their parent’s IXTQ scores.
Results: The greatest HRQOL concern for each child with IXT and their parent was “Worry about eyes.” Lower child IXTQ scores were associated with a larger deviation angle. “Shutting one eye when it is sunny” and “Waiting for their eyes to clear up” were more common in children with a larger deviation angle. The parent IXTQ scores were lower than the child ones and showed a positive correlation with child IXTQ scores. Lower parent IXTQ scores were associated with poor distance stereoacuity.
Conclusion: The HRQOL of IXT children was closely related to that of their parents. A larger deviation angle and worse distance stereoacuity function may predict more-negative impacts on children and their parents, respectively.
Molecular dynamics study of unexpected hydrate formation from supersaturated methane solution at low pressure
Authors: Belosludov Vladimir R. , Gets Kirill V. , Zhdanov Ravil K. , Bozhko Yulia Yu. , Belosludov Rodion V. , Chen Li-Jen , Kawazoe Yoshiyuki 
Published: November 2022

Abstract
Using molecular dynamics the evolution of a metastable solution for "methane + water" was studied for various methane concentrations at T = 270 K and P = 1 bar. We have found the intriguing behavior of the system: the formation of hydrate-like structures is observed in the concentration range. This formation was studied on different time scale levels. We have shown that this process is a collective process of H-bond network reorganization in solution at the initial stage (0–1 ns) until the first fluctuating cavities appear. This is associated with a preceding increase of tetrahedrality the water molecules. The molecules with a high degree of tetrahedrality are distributed uniformly throughout the volume. The formation of small and large fluctuation cavities present in the structure I (sI) appears before steady hydrate growth begins. Later, the constantly present hydrate cavities become the foundation for a few independent hydrate nucleation centers. This new mechanism of hydrogen bond network reorganization depends on the entropy of the cavity arrangement of the guest molecules in the hydrate lattice and leads to the hydrate growth.
Use of inhaled nitric oxide prognosticates poor survival in severe ARDS with venovenous ECMO: a retrospective analysis
Authors: Muenster Stefan , Nadal Jennifer , Schewe Jens-Christian , Ehrentraut Heidi , Kreyer Stefan F. X. , Putensen Christian , Ehrentraut Stefan F. 
Published: November 2022

Abstract
Background
Inhaled nitric oxide (iNO) has not been sufficiently evaluated in adult patients with severe acute respiratory distress syndrome (ARDS) under venovenous extracorporeal membrane oxygenation (VV ECMO) support.
Objectives
This study aims to assess survival in patients with severe ARDS under VV ECMO with iNO.
Methods
Of the 657 patients under ECMO, 292 under VV ECMO were evaluated. Outcomes in the iNO group (n = 55) were compared with those of the propensity-matched (based on age, sex, height, and sequential organ failure assessment score at admission) control cohort (55 patients under VV ECMO without iNO). Median survival was analyzed using the Kaplan–Meier method, whereas the hazard ratio for in-hospital mortality with iNO use was analyzed using the proportional hazards model.
Results
Weaning failure from VV ECMO was higher in the iNO group (69.7% vs. 47.3%, p = 0.0033). Thirty percent of patients responded sufficiently to iNO, showing a lower pulmonary arterial pressure. Survival was lower in the iNO group compared with the control cohort (22 vs. 109 days, p = 0.0222). The length of stay in the intensive care unit (ICU) (23 vs. 33 days, p = 0.0186) and total hospital stay (27 vs. 35 days, p = 0.0085) were shorter with iNO use. Survival was lower and the risk of mortality (hazard ratio, 1.8; 95% CI 1.1–3.0, p = 0.027) was higher in patients with iNO administration.
Conclusions
Inhaled NO in patients under VV ECMO is a strong predictor of shorter median time of survival and in-house mortality.
Hereditary Diffuse Gastric Cancer in a Japanese Family with CDH1 Mutation: Three Individuals Undergoing Total Gastrectomy Following Presymptomatic Genomic Diagnosis
Authors: Muranaka Futoshi , Kise Emiko , Tokumaru Shigeo , Kitazawa Masato , Miyagawa Yusuke , Suga Tomoaki , Uehara Takeshi , Iwaya Mai , Kobayashi Shota , Sato Midori , Gomi Daisuke , Yamada Hidetaka , Sugimura Haruhiko , Kosho Tomoki , Soejima Yuji , Koizumi Tomonobu 
Published: November 2022

Abstract
Background;
Germline pathogenic variants in the E-cadherin gene CDH1 cause hereditary diffuse gastric cancer (HDGC), which is an autosomal dominant cancer syndrome, accounting for 1% of all gastric cancers. HDGC harboring a CDH 1 variant is extremely rare in Japan.
Method;
Here, we describe four cases of HDGC in a single Japanese family.
Results;
The proband exhibited advanced and metastatic gastric cancer, and was found to have a previously reported heterozygous frameshift variant in CDH1 (NM_004360.3:c.1009_1010del:p.Ser337Phefs*12). Five at-risk relatives underwent presymptomatic molecular testing after careful genetic counseling, and three were molecularly diagnosed as positive for the variant. Esophagogastroduodenoscopy was performed in three relatives, which revealed abnormal small pale mucosal patches, small ulcerative lesion, and no abnormal findings, respectively. Random and targeted biopsies were compatible with the pathological diagnosis of HDGC. They underwent total gastrectomy and Roux-en-Y reconstruction with D2 lymph node dissection.
Conclusion;
In the management of HDGC, multidisciplinary and family-oriented approaches are critical, including genetic analysis of CDH1 variants in the proband, presymptomatic diagnosis of at-risk relatives through careful genetic counseling, and random biopsy-based surgery or prophylactic total gastrectomy.
Early Safety and Efficiency Outcomes of a Novel Standardized Interdisciplinary Procedure of Combined Laparoscopic Resection Rectopexy with Laparoscopic Sacrocolpopexy to Treat Obstructive Defecation Syndrome in Women with Pelvic Organ Prolapse
Authors: Ludwig Sebastian , Madukkakuzhy Joshy , Hernandez Alberto Vega , Otten Jakob , Ulrici Christoph , Karapanos Leonidas , Rudroff Claudia 
Published: November 2022

Abstract
Purpose
Obstructive defecation syndrome (ODS) defines a disturbed defecation process frequently associated with pelvic organ prolapse (POP) in women. It substantially compromises quality of life. Conservative treatment options are limited. Surgical interventions are characterized by a variety of individual approaches. This study standardized laparoscopic resection rectopexy (L-RRP) combined with laparoscopic sacrocolpopexy (L-SCP) in an interdisciplinary setting. Additionally, offering both synthetic mesh (SM) and biological mesh (BM) material for L-SCP is a novel treatment alternative, particularly for premenopausal women.
Methods
Women with ODS combined with POP underwent surgery using an interdisciplinary approach. The primary endpoint was postoperative morbidity and mortality measured by the Clavien-Dindo classification (CDC). Further endpoints were improvement in bowel evacuation, POP, and urinary incontinence at a median follow-up period of > 12 months.
Results
Of a total of 44 patients, 36 patients were treated with L-RRP combined with L-SCP (28 patients with SM, 8 patients with BM). CDC grades and the frequency of postoperative complications were low. Clinical outcomes for ODS, bowel dysfunction, and fecal control improved significantly. Anatomical outcome for POP was significantly better with 28 (78%) patients having POP-Q stage 0 after surgery. 17 patients (47%) had urinary incontinence before surgery, which was restored in 14 patients (82%). No de-novo urinary incontinence appeared.
Conclusion
The interdisciplinary surgical approach with L-RRP and L-SCP is safe, feasible, and effective in treating women with ODS and POP. The use of a biomesh was established as an effective alternative to the SM and offers an additional and new uterine-preserving option.
High molar activity [18F]Tetrafluoroborate synthesis for Sodium Iodide Symporter imaging by PET.
Authors: Soloviev Dmitry , Dzien Piotr , Mackintosh Agata , Malviya Gaurav , Brown Gavin , Lewis David 
Published: November 2022

Abstract
Background. Sodium iodide symporter (NIS) imaging by positron emission tomography is gaining traction in nuclear medicine, with a number of human studies recently published using fluorine-18 labelled tetrafluoroborate ([18F]TFB). Clinical success of any radiotracer relies heavily on its accessibility, which in turn depends on the availability of robust radiolabelling procedures providing a radiotracer in large quantities and of high radiopharmaceutical quality.
Results. Here we publish improved radiolabelling of and quality control procedures for [18F]TFB with high molar activity. The use of ammonium hydroxide for [18F]fluoride elution, of commercially available boron trifluoride-solvent complex as precursor and removal of unreacted [18F]fluoride on Florisil solid-phase extraction cartridges resulted in the reliable production of [18F]TFB on SYNTHRA and FX-FN automated synthesizers with radiochemical yields in excess of 30%, radiochemical purity in excess of 98% and molar activities in the range of 35 -217 GBq/μmol at the end of synthesis.
Conclusions. A novel automated radiosynthesis procedure for [18F]tetrafluoroborate has been developed that provides radiotracer with high molar activity. PET scanning of a mouse lung tumour model carrying an NIS reporter gene rendered images of high quality and improved sensitivity.
Vision-driven Deep Reinforcement Learning for Electronic Components Robotic Insertion Tasks
Authors: Bartyzel Grzegorz Marcin , Półchłopek Wojciech , Rzepka Dominik 
Published: November 2022

Abstract
One of the challenges of robotics in the modern manufacturing industry is assembly task. The manufacturing industry requires various insertion tasks, from peg-in-hole tasks to electronic parts assembly. Nowadays, robotic solutions for this problem often use the conventional methods. In those methods, the industrial robot is controlled by the hybrid force-position control and performs preprogrammed trajectories, such as a spiral path. However, electronic parts require more sophisticated techniques due to their complex geometry and susceptibility to damage. We propose a vision-driven method based on reinforcement learning (RL) for assembling electronic parts. In our approach, the input image for the RL agent is acquired from two cameras mounted to the robot's end-effector. In this work, we also analyze the influence of the observation modalities on the RL's agent performance metrics, such as insertion success rate and average assembly time. Results show that visual information acquired from a double-camera vision system significantly improves the RL method's robustness on the position disturbance in insertion tasks. The proposed method in this work outperforms conventional methods, such as random search, spiral search, and straight-down insertion in terms of success rate and robustness for the robot's initial position disturbances. Moreover, our approach is more robust for disturbance than a method that uses an external camera or a single camera mounted to the robot's end-effector for image acquisition.
Expression and prognostic value of LRFN family genes in prostate cancer by integrated bioinformatics analysis
Authors: Pan Haojie , Liu Suwei , Wu Mingzhe , Zhou Anan , Xiao Xi , Tian Junqiang 
Published: November 2022

Abstract
To analysis the expression of Leucine‑rich repeat fibronectin type 3 domain containing family genes(LRFNs) in prostate cancer (PCa) and its relationship with clinical features and prognosis. The PCa gene expression profiles were collected from UCSC Xena, and corresponding clinical information was extracted from the cBioPortal website. R and Cytoscape software and online tools such as HPA, cBioPortal, LinkedOmics, String, Metascape, and TIMER2 were used for bioinformatics analysis. LRFN1/2 were upregulated in various tumor tissues, including PCa, while the expression of LRFN3/4/5 were downregulated. LRFN1/2/4 were associated with disease-free survival (DFS) in PCa. LRFN4 was also associated with Gleason score and TNM staging. The protein-protein interaction (PPI) network analysis indicates that the LRFN protein family binds to the Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation protein or 14-3-3 protein(YWHA). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the LRFNs and its 250 co-expressed genes are involved in protein binding and transmembrane transport, cell cycle regulation, and the PI3K-Akt pathway, etc. Besides, upregulation of the LRFN5 was correlated with the increased infiltration of immune cells such as B cells, CD4+ T cells, CD8+ T cells, macrophages, neutrophils, dendritic cells, and the increased expression levels of various immune molecules. LRFNs have an essential role in the development and progression of PCa. LRFN1/2 expression is upregulated in PCa and LRFN3/4/5 expression is downregulated; LRFN1/2/4 may serve as potential prognostic biomarkers in PCa. LRFN3 and LRFN5 may be involved in regulating alterations of the immune microenvironment in PCa tumors.
Modelling of cooling effects in tool grinding using numerical simulations of the wetting behaviour of grinding wheels
Authors: Wichmann Marcel , Eden Michael , Zvegincev Dennis , Wiesener Frederik , Bergmann Benjamin , Schmidt Alfred 
Published: November 2022

Abstract
In manufacturing of cylindrical cemented carbide tools, helical flute grinding is an important process step. Process planning and the use of cooling lubricants are defining factors for process performance. Therefore, finding optimal parameters and cooling conditions is essential because they characterize the properties of the boundary zone, e.g. residual stresses. In this paper, grinding oil droplet experiments are compared with simulation results to describe the wetting behavior of different grinding wheel based on their specifications (grain size, bonding structure, and wetting status). More specifically, finite element simulations of the thin-film equation are used to identify corresponding slip parameters that will be used in more complex 3D fluid-dynamic simulations via the Joseph-Beavers condition. The results show that both the bonding and, to a lesser degree, the grain size have an influence on the wetting behavior. This presents an intermediate step in getting a better understanding of the cooling properties of lubricants in grinding processes, where the wetting effectiveness plays an important role for the heat transport.
Dermatobacter hominis gen. nov., sp. nov., a new member of the family Iamiaceae, isolated from keratin epidermis
Authors: Jo HyungWoo , Baek Chaeyun , Heo Young Mok , Kim Hye-Been , Kang Seunghyun , Mun Seyoung , Oh Yunseok , Han Kyudong , Riesco Raúl , Trujillo Martha E. , Lee Dong-Geol 
Published: November 2022

Abstract
A non-motile, novel actinobacterial strain, designated Kera-3T, was isolated on 1/10 diluted R2A agar from human keratinocytes. Strain Kera-3T was Gram-stain-positive, aerobic and rod-shaped. The whole-cell hydrolysis of amino acids contained meso-DAP, alanine, and glutamic acids. The main menaquinone was MK-9 (H8). The major fatty acids were C16:0 and C18:1 ω9c. The major phospholipids were diphosphatidylglycerol and aminophospholipid, along with an unidentified phosphoglycolipid and an unidentified aminophosphoglycolipid. The G + C content of the genomic DNA was 70.5 % based on the complete genome sequence. Phylogenetic analyses of the 16S rRNA gene sequence and core-gene based on amino acid sequence clearly showed that strain Kera-3T formed a new lineage in the family Iamiaceae, being the closest neighbour Rhabdothermincola sediminis SYSU G02662T with a 91.19% 16S rRNA gene sequence identity. Phylogenetic data was supported with a comparative genomic study of the predicted general metabolism and carbohydrate active enzymes. Based on physiological and biochemical characteristics data analysis. Strain Kera-3T can be distinguished from known genera in the family Iamiaceae and represents a novel genus and novel species. Therefore, the name Dermatobacter hominis gen. nov., sp. nov. is proposed with the type strain being Kera-3T (= KACC 22415T = LMG 32493T).
Tumour cell-induced senescence-like state of macrophages plays a pivotal role in tumour stable growth after tumour cell emergence in immunocompetent condition
Authors: Wada Haruka , Otsuka Ryo , Germeraad Wilfred , Murata Tomoki , Kondo Toru , Seino Ken-ichiro 
Published: November 2022

Abstract
Background: The cancer stem cell theory proposes that tumour formation in vivo is driven only by specific tumour-initiating cells having stemness; however, clinical trials conducted to test drugs that target tumour stemness have provided unsatisfactory results thus far. Recent studies showed clear involvement of immunity in tumours; however, the requirements of tumour-initiation followed by stable growth in immunocompetent individuals remain largely unknown.
Methods: To clarify this, we used two similarly induced glioblastoma lines, 8B and 9G. They were both established by overexpression of an oncogenic H-RasL61 in p53-deficient neural stem cells. In immunocompromised animals in an orthotopic transplantation model using 1000 cells, both show tumour-forming potential. On the other hand, although in immunocompetent animals, 8B shows similar tumour-forming potential but that of 9G’s are very poor. This suggests that 8B cells are tumour-initiating cells in immunocompetent animals. Therefore, we hypothesised that the differences in the interaction properties of 8B and 9G with immune cells could be used to identify the factors responsible for its tumour forming potential in immunocompetent animals and performed analysis.
Results: Different from 9G, 8B cells induced senescence-like state of macrophages around tumours. We investigated the senescence-inducing factor of macrophages by 8B cells and found that it was IL-6. Such senescence-like macrophages produced Arginase-1, an immunosuppressive molecule known to contribute to T cell hyporesponsiveness. The senescence-like Mφs highly expressed CD38, a nicotinamide adenine dinucleotide (NAD) glycohydrolase associated with NAD shortage in senescent cells. The addition of nicotinamide mononucleotide (NMN), a NAD precursor, in vitro inhibited to induction of macrophage senescence-like phenotype and inhibited Arginase-1 expression resulting in retaining T cell function. Moreover, exogenous in vivo administration of NMN after tumour inoculation inhibited tumour-initiation followed by stable growth in the immunocompetent mouse tumour model.
Conclusions: We identified one of the requirements for tumour-initiating cells in immunocompetent animals. In addition, we have shown that tumour growth can be inhibited by externally administered NMN against macrophage senescence-like state that occurs in the very early stages of tumour-initiating cell development. This therapy targeting the immunosuppressive environment formed by macrophage senescence-like state is expected to be a novel promising cancer therapeutic strategy.
Meta-transcriptional Detection of Rickettsia canadensis from Ixodes persulcatus in China
Authors: Shi Mingjie , Qin Tong , Liu Zhitong , Feng Hao , Sun Yi , Zhang Meina 
Published: November 2022

Abstract
Genus Rickettsia associated to blood-feeding arthropods are usually pathogenic when transmitted to vertebrates. These species include the agents of acute human disease such as typhus and Rocky Mountain spotted fever. However, many other Rickettsia have been uncovered with uncertain pathogenic to vertebrates in recent surveys. It is therefore perhaps more appropriate to consider these Rickettsiaas endosymbionts that are transmitted vertically in invertebrates, and secondarily as pathogens of vertebrates. Rickettsia canadensis, an ancestor species in genus Rickettsia, had been well characterized for its peculiar genome size and genes’ contents bridging the pathogenic Rickettsiaand non-pathogenic ones. However, there is few research on the ancient rickettsia species in China which may seriously influence our pursue for the evolutionary nature of Rickettsia in China.
In the present study, R. canadensis was detected from Ixodes persulcatusticks from forest areas of both Heilongjiang province and Inner Mongolia Autonomous Region in northern China. The molecular evidences from both 16S rRNA gene and 3 arrays of gene transcripts strongly indicated the natural infection of R. canadensis occurs in Ixodid ticks in China. The gene transcripts including rpoB-rpoC-rpoC/160KDa-leucyl aminopeptidase-hypothetical protein-leucyl aminopeptidase-aspartate tRNA ligase, RlmE-Omp 1-Peptidase M50-nusB, dnaK-dnaJ-ChaBfamily protein-BamD-RecN-Carboxypeptidase M32 revealed from I. persulcatus suggested the intensive interactions between R. canadensis and. host ticks, which would provide scientific clues to highlight the possible evolutionary trajectory of Rickettsia species in China.
Can China’s Hu Huanyong Line be broken to achieve common prosperity in the new development stage?
Authors: zhao dandan , wu fuxiang , huang jiayi 
Published: November 2022

Abstract
We analyse the causes of unbalanced development on both sides of the Hu Line and seek to narrow the gap in regional economic development levels. Achieving common prosperity is the fundamental principle, ultimate goal and basic requirement of socialism with Chinese characteristics. In this study, a total of 48 cities on both sides of the Hu Line are selected as samples, and an assessment system covering 18 indicators based on 8 aspects is constructed. A regression discontinuity method is adopted to test the differences in regional development on both sides of the Hu Line. The results show that the breakpoint effect on both sides of the Hu Huanyong Line is very obvious and that there is a significant imbalance in development, which is mainly reflected in eight aspects: population, economic output, finance, public finance, educational resources, employment, medical facilities, and urban development and construction. The results suggest that a large amount of work should be performed to increase fiscal support, strengthen public facility construction, improve medical and health levels, enhance investment in human capital and education, and accelerate the urbanization processes on the left side of the Hu Huanyong Line.