bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Hf-rTMS on rehabilitation of chronic pelvic pain syndrome under different intensities : study protocol for a randomized controlled trial.
Authors: Wang Mengyang , rui xia , Jiao Shi , Yang Chunhua , Zhang Yongqing , Xu Zhengxian , Yu Cancan , Wu Ziyi , Wang Min , Chen Shangjie , qu hongdang 
Published: August 2022

Abstract
INTRODUCTION: Chronic pelvic pain syndrome (CPPS) is one of the most common concentrated pain conditions in urology and gynecology. Nearly one in seven women of worldwide suffer from CPPS each year. CPPS often requires a combination of treatments. An increasing number of studies have shown good therapeutic effects of rTMS on CPPS, however there is a lack of a reasonable treatment intensity reference.The main objective of this trial is to observe the effect of high-frequency rTMS at different intensities on CPPS.METHODS AND ANALYSIS: In this prospective, double-blinded randomized controlled trial of outcome assessment, a total of 63 female CPPS participants will be recruited and randomized (1:1:1) to high intensity rTMS, low intensity rTMS, and routinely treatment control group. The control group will be received a ten-day course of conventional pelvic floor rehabilitation, including pelvic floor muscle bioelectro-feedback therapy, pelvic floor magnetic therapy, Hydrosun light therapy. On the basis of conventional treatment, participants in the high intensity group will be received 10Hz with 110%RMT for a total of 15000 pulses; participants in the low intensity group will be received 10Hz with 80%RMT with 15000 pulses. Primary outcome includes visual analogue scale (VAS), The Female Urogenital Pain Index (GUPI), Zung self-rating anxiety scale (SAS) and Zung self-rating depression scale (SDS), and secondary indicators of pelvic floor muscle surface EMG will be measured at baseline and after completion of treatment.ETHICS AND DISSEMINATION: This study is approved by the Ethics Committee of Bao 'an People's Hospital, Shenzhen, Guangdong Province (approval number: BYL20211203). The results will be submitted for publication in peer-reviewed journals and disseminated at scientific conferences.TRIAL REGISTRATION: Chictr.org.cn, ID: ChiCTR2200055615. Registered on 14 January 2022, http://www.chictr.org.cn/showproj.aspx?proj=146720.
The Effect of Propolis Addition Into Laying Hen Diet on Performance, Serum Lipid Profile and Liver Fat Rate
Authors: BÖLÜKBAŞI Canan , Ürüşan Hilal , Yıldırım Betül Apaydın 
Published: August 2022

Abstract
The aim of this study was to evaluate the effect of propolis (P) on performance egg quality parameters, serum lipid profile, some liver enzymes and liver fat ratio.. 120 Lohmann (LSL) laying hens were divided into 5 groups and each group consisted of 6 subgroups. The control group was fed basal diet. The other groups were fed high-energy diets to induce fatty liver, and 0, 100, 200 and 300 mg/kg propolis were supplemented to high-energy feeds, respectively. During the eight-week trial, feed and water were given as adlibitum. It was determined that egg production and feed convertion ratio were decreased in the high-energy feed group without the addition of propolis. The highest egg production was found in HE + 100 mg/kg P and HE + 200 mg/kg P groups. It was found that liver fat ratio were higher in the group fed with HE + 0 mg/kg P feed (P < 0.01) than other groups. But the addition of P decreased the liver fat rate significantly. The highest VLDL, TG and LDL values were found for HE + 0 mg/kg P group. The addition of 200 mg/kg propolis to high-energy feed increased GSH, SOD, CAT and GPx values. Conclusion, high-energy feed adversely affected egg production and liver fat ratio, but the addition of 100 or 200 mg/kg propolis improved egg production and decreased liver fat ratio.
Multibiomarker Approach in Fish to Assess a Heavily Polluted Brazilian Estuary, Guanabara Bay
Authors: Freire Marina Moreira , Gomez Cristina , Moreira Josino Costa , Arias Ana Rosa Linde 
Published: August 2022

Abstract
Brazil’s Guanabara Bay (GB), located in Rio De Janeiro, is a deeply contaminated, eutrophic waterbody that has a reduced environment that challenges the understanding of the effects of pollutants on the biota. This paper presents an integrated strategy to evaluate the impact of contamination, utilizing a multibiomarker approach in the following two fish species: corvine (Micropogonias furnieri) and burrfish (Chilomycterus spinosus). The integrated strategy is comprised of a general biomarker of fish’ physical condition, the condition factor (CF), and specific biomarkers of pollutant exposure such as metallothionein (MT), acetylcholinesterase (AChE) activity and biliary polycyclic aromatic hydrocarbons (PAH) metabolites. The results showed that both fish species presented significantly lower CF values, higher levels of MT, and lower AChE activities at the bay. Levels of PAHs were also higher in GB for both fish species; however, a different behavior concerning the origin of the PHAs was also identified. Therefore, the importance of using more than one sentinel species in the environmental assessment of complex pollution situations is illustrated by the results. Our findings indicate that pollutants are affecting the biota of the estuary. The present study shows the utility of incorporating a set of biomarkers to outline the consequences of anthropogenic intakes in water bodies under complex contaminated conditions.
Variation of heat flux and ecological impact on fish reproduction in the middle reaches of Yangtze River
Authors: Guo Wenxian , Chen Wenxiong , He Ning , Wang Hongxiang 
Published: August 2022

Abstract

River heat flux (HF) regime has been significantly affected by anthropogenic activities and climate variation, and it is of great significance to deeply explore intrinsic driving mechanisms and ecological effects. This study uses the middle reaches of the Yangtze River as its research area and, by constructing the wavelet model and the IHA-RVA model, quantifies the evolution mechanism and internal law of "flow- water temperature (WT) - HF" over the past four decades and investigates the effects of Three Gorges Dam on the ecological reproduction of "Four Major Chinese Carp". The results show that, (1) Flow and WT have three change cycle scales; The overall hydrologic variations of flow and WT were 64% and 62%, respectively, close to high variation. (2) The overall HF shows a decreasing trend from 1983 to 2019, with significant changes in HF in spring and winter regulated by the Three Gorges Reservoir; The basin flow-WT-HF relationships exhibit a hysteretic pattern, with the maximum WT occurring one month after the peak HF and flow. (3) The "Four Major Chinese Carp" natural breeding season is closely related to the time when the WT reaches 18°C; HF is a vital habitat factor that influences fish spawning and reproduction.
Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff
Authors: Gopcevic Kristina , Grujić Slavica , Arsenijevic Jelena , Džamić Ana , Veličković Ivona , Izrael-Živković Lidija , Medić Ana , Mudrić Jelena , Soković Marina , Đurić Ana 
Published: August 2022

AbstractThis study aimed at investigating biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC-PDA/MS analyses for the aqueous (A1-ste, leaves and flowers, A2-leves and flowers) and ethyl-acetate (E1-stem, leaves and flowers, E2-leaves and flowers) extracts obrtained by ultrasound-assisted extraction allowed the identification of thirty four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). Total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (27±2.4 mg Gallic Acid equivalents /g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20±10 µg/mL), ABTS (2.834±0.02 mg Ascorbic Acid/g), FRAP (1.922±0.03 mmol Fe2+/mg), and total reducing power tests (16.4±1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70-1.99 mg/mL and 1.99-3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All obtained results implied the good potential of investigated extracts to be used as an effective preservatives and functional ingredients in food products and dietary supplements.
Revamping an aquatic irritant to fortune-Utilizing jellyfish for the extraction of hyaluronic acid and its characterization
Authors: Raj Rehana , K.R Sreelakshmi , V.A Minimol , Ninan George , K.K Asha 
Published: August 2022

Abstract
Hyaluronic acid (HA) is a valuable bioactive polysaccharide that is having numerous application in biomedical and pharmaceutical disciplines. It is normally characterized by the viscous slippery glue which is having a diversified range of relevance. Extraction of hyaluronic acid has been standardized from various sources like pig, rabbit, oxes and human, but these methods have certain limitations like low yield and also require the killing of these animals which is against the experimental ethics. Jellyfish production are now increasing widely and sufficient studies on proper utilization of it is still under progress. This species are known to cause devastation to the fishing vessels, boats and gears. Also the isolation of hyaluronic acid from marine sources are to be exploited further, which is considered to be extensively diverse. Even though numerous methodologies are adopted for its extraction, the impediment lies in the yield and molecular weight of this natural molecule. Hence some minor modification in the existing procedure should be followed. In this present study, isolation and structural characterization was carried out for the HA extracted from jellyfish. Yield obtained is one of the major constrains observed in this study. Further confirmation with FT-IR, Viscosity and Spectrophotometer analysis were also carried out. Protein contamination was not observed as the sample was subjected to SDS PAGE. was done to confirm the presence of any protein contamination. In-vitro moisture absorption studies conducted also indicated a better moisture retention capacity for the extracted compound.
“He’s a Little Skinny and He’s a Little Wide.”: A Mixed Design Investigation of American Indian Student Perceptions of Healthy Bodies
Authors: Cothran Donetta , Kulinna Pamela 
Published: August 2022

Abstract
BackgroundChildhood is a critical developmental time of nutrition and physical activity patterns, yet little is known about what children know and believe. Even less is known about non-majority cultures like American Indian youth. The purpose of this study was to explore American Indian students’ understandings of nutrition and physical activity.MethodsNinety American Indian students in grades 3-12 were interviewed. The interview included an 8-point body size chart. Numerical data were analyzed via t-test statistics while a constant comparison process and analysis was used for the interview data.ResultsStudents rated approximately 85% of students in Category 5 or smaller on the scale while placing 60% of adults at or above that size. There was a general trend of a larger body type for boys seen as healthy compared to that for girls. Students generally believed that their classmates were larger than the healthy body size.For students, a healthy body was the result of compliance with “eat right and exercise” rules. They exhibited little understanding of nutrition or physical activity and there were few developmental differences in understanding. Health was a corporeal concept and violators of the eat right and exercise rules were seen as lazy.ConclusionsStudents held limited, narrow, and corporeal focused notions of health that focused on simple rules. People who violated the rules were described as “lazy”, a concept that seemed to underlie multiple constructs and a finding that holds true in other investigations. Students also reported few adult role models, a topic that should be explored more with expanded family groups to better represent the multi-generational (e.g. grandparents, uncles, aunts) family housing common in the community. The findings are limited to a single American Indian community and a mixed design of relatively small numbers. This addition to the literature from a non-majority cultural group expands our knowledge of student perspectives on health. These findings can be used to create more effective curricula and interventions. Schools need more effective, but also alternately framed approaches that promote broader views of health as part of total wellness.
Data Imputation Model for Internet of Medical Things (IoMT) Using Nonlinear Autoregressive Exogenous (NARX) network Model
Authors: S Dhamodharavadhani , Rathipriya R 
Published: August 2022

Abstract
The objective of this paper is to propose a data transmission reduction approach using Nonlinear Autoregressive Exogenous (NARX) network based prediction model to minimise energy consumption in sensor nodes (SNs) and base stations (BS). The NARX based prediction model is used to impute the missing sensor values by the base station. The imputation of missing transmitted sensor data is compensated by the base station using historical sensor data and sensor values of the interrelated sensor data in Internet of Medical Things (IoMT). The purpose of this paper is to achieve energy conservation by reducing the amount of redundant sensor data transmitted over the IoMT when data is not received at Time stamp‘t’.
Comparison and optimization of transfection methods for human lymphoblast TK6 cell line
Authors: Ewunkem Akamu Jude , Agee Kyle 
Published: August 2022

AbstractTransfection has recently gained attention in the field of biomedical research due to its ability to manipulate gene expression. Every mammalian cell type has a characteristic set of requirements for optimal transfection. Some cells can be difficult to transfect and requires optimization for successful transfection. Human lymphoblast TK6 cell line, an important cell line for genotoxic studies, are known to be extremely hard to transfect. Transfection methods for human lymphoblast TK6 is increasingly important. To accomplish this, TK6 human lymphoblasts were transfected with plasmid constructs that expressed Green fluorescent Protein (GFP) and NanoLuc® activity. We compared the transfection efficiencies of three commercially available transfection reagents including Amaxa 96-well Nucleofection procedure using various solutions (SF, SE and SG), Lipofectamine LTX, and Metafectene Pro®. The transfection efficiencies, and toxicity of various reagents were tested by fluorescence microscopy, luciferase activity, and cell viability assays. Amaxa 96-well Nucleofection Solution SF was identified as the best transfection reagent due to its relatively high luciferase activity, acceptable cell viability (80%), and GFP transfection efficiency (80%). Efficient conditions for transfection utilized with this reagent included 0.4µg of plasmid DNA, 1.8 x 105cells, and the DS 137 nucleofector program.
Comparison of the analgesic effect of Ketamine and Midazolam with Apotel and Ketorolac in renal colic patients: a clinical trial
Authors: Bahrami Anvar , Zareie Bushra , Jabbari Latife , Zamanimehr Nahid , AzizKhani Leila 
Published: August 2022

Abstract
Background: This study aimed to determine the effect of the Ketamine and Midazolam combination and compare it with the Apotel and Ketorolac combination in pain management of patients with renal colic in the emergency section.In this double-blind clinical trial study, 200 renal colic patients admitted to the ED with more than 8 NRS* (Numerical Rating Scale) of primary pain were divided into two groups by random blocking: one group received intravenous Ketamine (0.4 mg/kg), and intravenous Midazolam (at a dose of 0.016 mg/kg) and the other group received intravenous Ketorolac (30 mg) and intravenous Apotel (15 mg/kg). After that, we measured patients' pain at 1, 5, 10, 15, 30, and 45 min after the procedure.Results: 124 (62.0%) of 200 patients were men. Initial pain scores were 9(10-9) for Ketamine + Midazolam and 10(10-9) for Apotel + Ketorolac.Linear regression was performed to compare the two groups' adjusted pain scores, correcting for initial pain. The ultimate pain score increased by.392 units for each unit of starting pain. Group and time had significant effects (5.553, -.035, P=.001, respectively).Apotel + Ketorolac had a higher mean pain score than Ketamine + Midazolam at all post-intervention time intervals. During the trial, both groups' discomfort decreased dramatically.Conclusions: The combination of Ketamine and Midazolam was more effective than Apotel and Ketorolac in relieving the pain in renal colic. Therefore, if routine medications are contraindicated, a combination of Ketamine and Midazolam is recommended for pain control in patients with renal colic.Trial registration: This trial was registered in the Iranian Registry of Clinical Trials with IRCT registration number of IRCT20200422047163N1 (https://www.irct.ir/trial/47370) and registration date of 2020-05-01.
Efficacy and auditory biomarker analysis of fronto-temporal transcranial direct current stimulation (tDCS) in targeting cognitive impairment associated with recent-onset schizophrenia: study protocol for a multicentric randomized double-blind sham-controlled trial
Authors: DONDÉ Clément , Bastin Julien , Pouchon Arnaud , Costes Nicolas , Fakra Eric , Galvão Filipe , Gay Aurelia , Haesebaert Frederic , Lamalle Laurent , Mérida Inès , Rigon Maxence , Schneider Fabien , Troprès Irène , Brunelin Jerome , Polosan Mircea 
Published: August 2022

Abstract
Background: In parallel to the traditional symptomatology, deficits in cognition (memory, attention, reasoning, social functioning) contribute significantly to disability and suffering in individuals with schizophrenia. Cognitive deficits have been closely linked to alterations in early auditory processes (EAP) that occur in auditory cortical areas. Preliminary evidence indicates that cognitive deficits in schizophrenia can be improved with a reliable and safe non-invasive brain stimulation technique called tDCS (transcranial Direct Current Stimulation). However, a significant proportion of patients derive no cognitive benefits after tDCS treatment. Further, the neurobiological mechanisms of cognitive changes after tDCS have been poorly explored in trials and are thus still unclear.Method: The study is designed as a randomized, double-blind, 2-arm parallel-group, sham controlled, 4-centers trial. Sixty participants with recent-onset schizophrenia and cognitive impairment will be randomly allocated to receive either active (n=30) or sham (n=30) tDCS (20-min, 2-mA, 10 sessions during 5 consecutive weekdays). The anode will be placed over the left dorsolateral prefrontal cortex and the cathode over the left auditory cortex. Cognition, tolerance, symptoms, general outcome and EAP (measured with EEG and multimodal MRI) will be assessed prior to tDCS (baseline), after the 10 sessions, and at 1- and 3-month follow-up. The primary outcome will be the number of responders, defined as participants demonstrating a cognitive improvement ≥Z=0.5 from baseline on the MATRICS Consensus Cognitive Battery total score at 1-month follow-up. Additionally, we will measure how differences in EAP modulate individual cognitive benefits from active tDCS and whether there are changes in EAP measures in responders after active tDCS.Discussion: Besides proposing a new fronto-temporal tDCS protocol by targeting the auditory cortical areas, we aim to conduct a Randomized Controlled Trial (RCT) with follow-up assessments up to 3 months and a large sample size. In addition, this study will allow identifying and assessing the value of a wide range of neurobiological EAP measures for predicting and explaining cognitive deficit improvement after tDCS. The results of this trial will constitute a step toward the use of tDCS as a therapeutic tool for the treatment of cognitive impairment in recent-onset schizophrenia.Trial registration: ClinicalTrials.gov NCT05440955. Prospectively registered on July 1st, 2022
Lead and Cadmium Contaminations in Soil and Wild Plant Species Along Karakorum Highway Northern Pakistan: Occurrence, Transformation and Human Health Risk
Authors: Ullah Hameed , Bakht Khush , Zeb Aurang , Ullah Sana , Sheng Yanqing , Nawab Javed 
Published: August 2022

Abstract
Lead and cadmium contaminations in soil and plant may indirectly result in human health risk. This study was to assess the lead (Pb) and cadmium (Cd) concentrations in soil and wild plants and its associated human health risk along the Karakorum Highway, Northern Pakistan. Results showed that the concentrations of both Pb and Cd in soil were above the permissible limits of Dutch standards, and the mean values in wild plant species were above the permissible limit according to WHO (1996). Metal bioaccumulation factor (BAF) and translocation factor (TF) were applied to assess metal uptake and transport through plant tissues. Among 30 wild plant species, merely 1 plant species (Debregeasia Saeneb) for Pb and 5 plant species (Dodonaea viscosa, Crysopoge gryllus, Debregeasia saeneb, Festuca hartmanii and Plantanus arientalis) for Cd showed BAF > 1. For the TF values, Pb in 11 plant species were > 1, while 19 plant species for Cd were > 1. The hazard index (HI) values for soil were observed < 1 with an order of ingestion > dermal > inhalation, indacting no adverse health effects. This study suggests that some wild plant species in Northern Pakistan present low BAF and TF values, those wild plants can be used for phytoremediation purposes.
hucMSC-derived exosomes ameliorate pressure ulcers by inhibiting HMGB1.
Authors: Yan Fei , Gong Meihua , Li Fu-Rong , Yu Li 
Published: August 2022

Abstract
Background:Pressure ulcers (PUs) are a type of chronic wound in the elderly population. Previous studies have shown that exosomes derived from stem cells contain cytokines and growth factors that affect tissue repair and can represent a therapeutic strategy for wound healing. Thus, fully understanding how to extract exosomes and their mechanism of action can help promote the management of chronic refractory wound healing as a new cell-free treatment model. Methods:In this study, we isolated exosomes from human umbilical cord mesenchymal stem cells (hucMSC-Exos) and examined their effects on wound healing. A total of 15 mice that were randomly divided into three groups, subjected to three ischemia–reperfusion (I-R) cycles and treated with different doses of hucMSC-Exos for different times. Quantitative real-time polymerase chain reaction (qRT–PCR) was used to analyze collagen mRNA levels in tissue samples. HMGB1 levels were examined by Western blotting and immunohistochemistry. α-SMA, CD34, and HMGB1 expression levels were compared to investigate the potential mechanisms. Results:We found that hucMSC-Exos could be taken up by fibroblasts and significantly regulated and improved fibroblast fibrosis and in PU wound healing in vivo. Furthermore, we observed that hucMSC-Exo treatment of PU wounds downregulated the expression of HMGB1, which was previously shown to have a deleterious effect on the wound healing process. Conclusions:Our findings indicate that hucMSC-Exos regulate the repair of PU wounds in part by inhibiting HMGB1 expression . Exosome treatment has provided new perspectives in regenerative medicine and trauma management.