bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revisão integrativa: distúrbios motores e o desenvolvimento da linguagem expressiva no Autismo
Authors: Freire Leonel Francisco de Oliveira , Leal Danielle Christina Lino , Cursino Maria Aparecida , Aires Anne Itamara Benigna Evangelista , Silva Marcos Daniel Oliveira e , Regis Matheus da Silva , Silva Marielen Ghedin da , Monteiro Maria das Dores de Holanda , Maia Raimunda Gerlane Lima , Souza Stheshy Vieira e , Lima Isabela Pinheiro Cavalcanti 
Published: January 2022

O Transtorno do Espectro do Autismo (TEA) consiste em um grupo de alterações do desenvolvimento neurológico e acomete, em nível global, cerca de 1 a cada 160 crianças. Os distúrbios motores são encontrados em até 79% dos indivíduos com autismo, e estão correlacionadas ao desempenho da linguagem e o comportamento social. Este estudo buscou responder a seguinte questão: As habilidades motoras podem estar correlacionadas ao desenvolvimento da linguagem expressiva no autismo? A pesquisa foi realizada no nas bases de dados Scielo, Pubmed, Lilacs, Scopus e Web Of Science. Foram utilizados termos em inglês, padronizados e extraídos dos Descritores em Ciências da Saúde (DECS) e do Medical Subject Headings (MeSH). Foram incluídos artigos originais, publicados em periódicos nacionais e internacionais nos anos de 2015 a 2020, nos idiomas inglês e português. A pesquisa resultou em 12.822 artigos, sendo incluídos 7 artigos após a seleção. Os caminhos percorridos até o desenvolvimento e a manifestação dos déficits motores e da linguagem no autismo ainda constituem uma lacuna para a ciência, no entanto, a literatura científica explorada permite estabelecer uma potencial relação de interferência bilateral entre esses fatores, permitindo amplificar o universo de investigações na abordagem motora destes déficits.
Aplicabilidade de Microrganismos Eficientes (ME) na Agricultura: uma revisão bibliográfica
Authors: Silva André Lopes da , Cordeiro Rogério Soares , Rocha Heliselle Cristine Ramires da 
Published: January 2022

Um dos desafios do setor agropecuário é a produção de alimentos em maiores dimensões e com a menor geração de resíduos sólidos nas etapas da cadeia produtiva, uma vez que estes podem acarretar sérios danos ambientais e nos solos agricultáveis. No entanto, várias medidas podem ser adotadas, e aquilo que seria um problema, pode se tornar uma solução para o agricultor. A técnica da compostagem tem sido uma alternativa eficiente para o reaproveitamento dos resíduos tanto de origem animal como vegetal, que ao passarem pelo processo de decomposição, podem ser incorporados ao solo. Visando uma maior aceleração e estabilização destes resíduos, tem se empregado a adição de Microrganismos Eficientes (MEs). Além de serem atuantes na decomposição, os MEs favorecem a disponibilização dos nutrientes e minerais do solo às plantas de forma eficaz. Deste modo, neste estudo realizou-se um levantamento bibliográfico sobre a produção acadêmica brasileira, no que se refere aos MEs. E, de forma mais específica, categorizar os diferentes manuscritos, a partir de critérios como o potencial biológico na agricultura e os diferentes tipos de substratos, onde MEs foram analisados. Com base nos resultados encontrados nessa revisão, conclui-se que os MEs possuem uma ampla utilização e benefícios para o desenvolvimento sustentável da agricultura. Percebe-se também, que diversas pesquisas comprovam as suas vantagens no processo de produção de substratos através do tratamento de resíduos com uso das técnicas de compostagem e vermicompostagem, possibilitando a reinserção dos mesmos nos solos cultiváveis, contribuindo com a melhoria das suas condições físico-químicas e biológicas.
Biologic Responses to House Dust Mite Exposure in the Environmental Exposure Unit
Authors: Hossenbaccus Lubnaa , Linton Sophia , Thiele Jenny , Steacy Lisa , Walker Terry , Malone Crystal , Ellis Anne K. 
Published: January 2022

Introduction: Allergic rhinitis (AR) is an inflammatory disease of the nasal mucosa that can be modeled using Controlled Allergen Exposure Facilities (CACF). Recently, we clinically validated the house dust mite (HDM) Environmental Exposure Unit (EEU) facility. In the current study, we aimed to assess biological responses in the blood following HDM exposure in the HDM-EEU.Methods: Fifty-five participants passed a screening visit, where they provided consent and completed a skin prick test (SPT), then attended a modest or higher HDM exposure session. Baseline and post-exposure blood samples were collected. Complete blood counts with differentials were measured, and isolated serum was used to determine Dermatophagoides farinae- and Dermatophagoides pteronyssinus-specific IgE (sIgE) and cytokine concentrations (IL-4, IL-5, IL-6, IL-10, IL-13, TNF-α).Results: HDM-allergic participants had significantly greater SPT wheal sizes than healthy controls. sIgE concentrations were significantly greater in allergic participants, with a strong correlation between Dermatophagoides farinae and Dermatophagoides pteronyssinus. Serum eosinophil counts were significantly decreased post-exposure for allergic participants. White blood cell, neutrophil, and lymphocyte counts were significantly increased for both allergic and non-allergic participants post-exposure. Serum IL-13 concentrations were significantly reduced post-exposure in allergics while TNF-α was significantly reduced in non-allergics.Conclusion: The HDM-EEU is a useful model for investigating biologic mechanisms of HDM-induced AR. Allergic participants produced measurable biological changes compared to healthy controls following allergen exposure, specifically with serum expression of eosinophils and related markers, namely IL-5, which promotes the proliferation and differentiation of eosinophils, and IL-13, a cytokine released by eosinophils. The exact mechanisms at play require further investigation.
Comprehensive Analysis of Differentially Expressed Profiles of mRNA N6-Methyladenosine in Colorectal Cancer
Authors: Li Na , Guo Qin , Zhang Qiao , Chen Bai-Jun , Li Xiao-An , Zhou Yan 
Published: January 2022

Aim: To comprehensively profile the landscape of the mRNA N6-methyladenosine (m6A) modification in human colorectal cancer (CRC).Methods: Methylated RNA immunoprecipitation sequencing (MeRIP-seq) was explored to compare the difference in mRNA N6-methyladenosine (m6A) methylation between CRC tissues and adjacent normal control (NC) tissue. RNA-sequencing (RNA-seq) was performed to transcribe differentially expressed mRNAs. Conjoint analysis of MeRIP-seq and RNA-seq data was conducted to predict RNA-binding proteins (RBPs).Results: MeRIP-seq identified 1110 differentially m6A methylated sites (DMMSs) and 980 differentially m6A methylated genes (DMMGs) in CRC, with 50.13% of all modified genes showing unique m6A-modified peaks in CRC. RNA-seq showed 915 upregulated genes and 1463 downregulated genes in CRC. QRT-PCR verified the RNA-seq results by detecting the expression of some mRNAs. Conjoint analysis of MeRIP-seq and RNA-seq identified 400 differentially m6A methylated and expressed genes (DEGs), and pathway analysis detected that DMMGs and DEGs were closely related to cancer. After analyzing these DMMGs and DEGs through the GEPIA database, we found that the expression of B3GNT6, DKC1, SRPK1, and RIMKLB were associated with prognosis, and the expression of B3GNT6 and RIMKLB were associated with clinical stage. 17 RBPs were identified based on the DMMGs and DEGs, among which FXR1, FXR2, FMR1, IGF2BP2, IGF2BP3, and SRSF1 were obviously highly expressed in CRC, and FMR1, IGF2BP2, and IGF2BP3 were closely related to methylation, and might be involved in the development of CRC.Conclusion: This study comprehensively profiled m6A modification of mRNAs in CRC, which revealed possible mechanisms of m6A-mediated gene expression regulation.
Construction of Electrochemical Aptamer Sensor Based on Pt-Coordinated Titanium-Based Porphyrin MOF for Thrombin Detection
Authors: Jiang Jiazi , Cai Quan , Deng Minghan 
Published: January 2022

In this work, a Pt-coordinated titanium-based porphyrin metal organic framework (Ti-MOF-Pt) was prepared by embedding single-atom Pt through strong interactions between the four pyrrole nitrogen atoms in the rigid backbone of the porphyrin. The synthesized Ti-MOF-Pt was characterized by TEM, XRD, FTIR and BET. Then, the Ti-MOF-Pt has been used for glassy carbon electrode surface modification and consequently used for construction of a thrombin aptamer sensor. The high surface area provides by MOF and excellent electrochemical property provided by Pt enhance the sensing performance. After optimization of amount of aptamer, hybridization time and specific reaction time, the fabricated aptamer sensor exhibited a linear relationship with the logarithm of the thrombin concentration in the range of 4 pM to 0.2 μM. The detection limit can be calculated as 1.3 pM.
Facing Diseases Caused by Trypanosomatid Parasites: Rational Design of Pd and Pt Complexes With Bioactive Ligands
Authors: Gambino Dinorah , Otero Lucía 
Published: January 2022

Human African Trypanosomiasis (HAT), Chagas disease or American Trypanosomiasis (CD), and leishmaniases are protozoan infections produced by trypanosomatid parasites belonging to the kinetoplastid order and they constitute an urgent global health problem. In fact, there is an urgent need of more efficient and less toxic chemotherapy for these diseases. Medicinal inorganic chemistry currently offers an attractive option for the rational design of new drugs and, in particular, antiparasitic ones. In this sense, one of the main strategies for the design of metal-based antiparasitic compounds has been the coordination of an organic ligand with known or potential biological activity, to a metal centre or an organometallic core. Classical metal coordination complexes or organometallic compounds could be designed as multifunctional agents joining, in a single molecule, different chemical species that could affect different parasitic targets. This review is focused on the rational design of palladium(II) and platinum(II) compounds with bioactive ligands as prospective drugs against trypanosomatid parasites that has been conducted by our group during the last 20 years.
Optimal Time of Collapse to Return of Spontaneous Circulation to Apply Targeted Temperature Management for Cardiac Arrest: A Bayesian Network Meta-Analysis
Authors: Duan Jingwei , Zhai Qiangrong , Shi Yuanchao , Ge Hongxia , Zheng Kang , Du Lanfang , Duan Baomin , Yu Jie , Ma Qingbian 
Published: January 2022

Background: Both the American Heart Association (AHA) and European Resuscitation Council (ERC) have strongly recommended targeted temperature management (TTM) for patients who remain in coma after return of spontaneous circulation (ROSC). However, the role of TTM, especially hypothermia, in cardiac arrest patients after TTM2 trials has become much uncertain.Methods: We searched four online databases (PubMed, Embase, CENTRAL, and Web of Science) and conducted a Bayesian network meta-analysis. Based on the time of collapse to ROSC and whether the patient received TTM or not, we divided this analysis into eight groups (&lt;20 min + TTM, &lt;20 min, 20–39 min + TTM, 20–39 min, 40–59 min + TTM, 40–59 min, ≥60 min + TTM and ≥60 min) to compare their 30-day and at-discharge survival and neurologic outcomes.Results: From an initial search of 3,023 articles, a total of 9,005 patients from 42 trials were eligible and were included in this network meta-analysis. Compared with other groups, patients in the &lt;20 min + TTM group were more likely to have better survival and good neurologic outcomes (probability = 46.1 and 52.5%, respectively). In comparing the same time groups with and without TTM, only the survival and neurologic outcome of the 20–39 min + TTM group was significantly better than that of the 20–39 min group [odds ratio = 1.41, 95% confidence interval (1.04–1.91); OR = 1.46, 95% CI (1.07–2.00) respectively]. Applying TTM with &lt;20 min or more than 40 min of collapse to ROSC did not improve survival or neurologic outcome [ &lt;20 min vs. &lt;20 min + TTM: OR = 1.02, 95% CI (0.61–1.71)/OR = 1.03, 95% CI (0.61–1.75); 40–59 min vs. 40–59 min + TTM: OR = 1.50, 95% CI (0.97–2.32)/OR = 1.40, 95% CI (0.81–2.44); ≧60 min vs. ≧60 min + TTM: OR = 2.09, 95% CI (0.70–6.24)/OR = 4.14, 95% CI (0.91–18.74), respectively]. Both survival and good neurologic outcome were closely related to the time from collapse to ROSC.Conclusion: Survival and good neurologic outcome are closely associated with the time of collapse to ROSC. These findings supported that 20–40 min of collapse to ROSC should be a more suitable indication for TTM for cardiac arrest patients. Moreover, the future trials should pay more attention to these patients who suffer from moderate injury.Systematic Review Registration: [https://inplasy.com/?s=202180027], identifier [INPLASY202180027]
Associations Among PCSK9 Levels, Atherosclerosis-Derived Extracellular Vesicles, and Their miRNA Content in Adults With Obesity
Authors: Macchi Chiara , Greco Maria Francesca , Favero Chiara , Dioni Laura , Cantone Laura , Hoxha Mirjam , Vigna Luisella , Solazzo Giulia , Corsini Alberto , Banach Maciej , Pesatori Angela C. , Bollati Valentina , Ruscica Massimiliano 
Published: January 2022

Background:Extracellular vesicles (EV) concentration is generally increased in patients with cardiovascular diseases, although the protective role of EVs in atherosclerosis has been reported. Among the specific cargo of EVs, miRNAs contribute to different stages of atherosclerosis. Aim of the present report has been to investigate, in individuals with obesity, the interplay among EVs derived from cells relevant for the atherosclerotic process (i.e., platelets, endothelium, monocytes/macrophages, and neutrophils), their miRNA content and proprotein convertase subtilisin/kexin type 9 (PCSK9), one of the main regulators of low-density lipoprotein receptor (LDLR).Methods and Results:EVs have been isolated from 936 individuals with obesity (body mass index = 33.6 ± 5.6 Kg/m2) and a raised cardiovascular risk (e.g., LDL-C = 131.6 ± 36.4 mg/dL, HOMA-IR = 3.1, and roughly 50% on anti-hypertensive medications). PCSK9 levels were negatively associated with EV count in the range 150–400 nm and with those derived from macrophages (CD14+), endothelium (CD105+), and neutrophils (CD66+). The association between PCSK9 and platelet-derived EVs (CD61+) was modified by platelet counts. PCSK9 was significantly associated with five EV-derived miRNAs (hsa-miRNA−362−5p,−150,−1244,−520b-3p,−638). Toll-like receptor 4 and estrogen receptor 1 were targeted by all five miRNAs and LDLR by four. The effect on LDLR expression is mainly driven by hsa-miR-150. Considering the implication of EV in atherosclerosis onset and progression, our findings show a potential role of PCSK9 to regulate EV-derived miRNAs, especially those involved in inflammation and expression of low-density lipoprotein receptor (LDLR) receptor.
Disruption of c-MYC Binding and Chromosomal Looping Involving Genetic Variants Associated With Ankylosing Spondylitis Upstream of the RUNX3 Promoter
Authors: Cohen Carla J. , Davidson Connor , Selmi Carlo , Bowness Paul , Knight Julian C. , Wordsworth B. Paul , Vecellio Matteo 
Published: January 2022

Background: Ankylosing Spondylitis (AS) is a common form of inflammatory spinal arthritis with a complex aetiology and high heritability, involving more than 100 genetic associations. These include several AS-associated single nucleotide polymorphisms (SNPs) upstream of RUNX3, which encodes the multifunctional RUNT-related transcription factor (TF) 3. The lead associated SNP rs6600247 (p = 2.6 × 10−15) lies ∼13kb upstream of the RUNX3 promoter adjacent to a c-MYC TF binding-site. The effect of rs6600247 genotype on DNA binding and chromosome looping were investigated by electrophoretic mobility gel shift assays (EMSA), Western blotting-EMSA (WEMSA) and Chromosome Conformation Capture (3C).Results: Interrogation of ENCODE published data showed open chromatin in the region overlapping rs6600247 in primary human CD14+ monocytes, in contrast to the Jurkat T cell line or primary human T-cells. The rs6600247 AS-risk allele is predicted to specifically disrupt a c-MYC binding-site. Using a 50bp DNA probe spanning rs6600247 we consistently observed reduced binding to the AS-risk “C” allele of both purified c-MYC protein and nuclear extracts (NE) from monocyte-like U937 cells. WEMSA on U937 NE and purified c-MYC protein confirmed these differences (n = 3; p &lt; 0.05). 3C experiments demonstrated negligible interaction between the region encompassing rs6600247 and the RUNX3 promoter. A stronger interaction frequency was demonstrated between the RUNX3 promoter and the previously characterised AS-associated SNP rs4648889.Conclusion: The lead SNP rs6600247, located in an enhancer-like region upstream of the RUNX3 promoter, modulates c-MYC binding. However, the region encompassing rs6600247 has rather limited physical interaction with the promoter of RUNX3. In contrast a clear chromatin looping event between the region encompassing rs4648889 and the RUNX3 promoter was observed. These data provide further evidence for complexity in the regulatory elements upstream of the RUNX3 promoter and the involvement of RUNX3 transcriptional regulation in AS.
The Role of Internal Limiting Membrane as a Biomarker in the Evolution of Myopic Traction Maculopathy
Authors: Fang Dong , Su Jindi , Chen Lu , Zhang Shaochong 
Published: January 2022

Purpose: To describe the longitudinal structural changes of myopic traction maculopathy (MTM) based on optical coherence tomography (OCT) and to detect biomarkers in the evolution of MTM.Methods: A retrospective study was conducted on patients with MTM as defined by OCT. A minimum follow-up of 6 months was necessary for study inclusion. The effects of comprehensive OCT-based structure on the evolution of MTM, the progression rates, and resolution rates of MTM were evaluated.Results: A total of 120 eyes (120 patients) were included with an average follow-up of 15.4 months. During the follow-up, MTM progressed in 32 eyes (26.67%). The most common pattern of progression observed was the increased extent of retinoschisis in 12 eyes. The multivariate analysis showed that the presence of MTM progression had a significant correlation with internal limiting membrane (ILM) detachment and retinoschisis involved the entire macula at baseline. Five eyes (4.17%) experienced MTM resolution, of which 2 eyes developed disruptions of detached ILM, two eyes developed disruptions of epiretinal membrane, and one eye developed partial posterior vitreous detachment. Eyes with foveal detachment showed the highest progression rate (41.67%) and highest resolution rate (16.67%) compared to the eyes with other foveal complications.Conclusion: ILM detachment is a risk factor for MTM progression and MTM resolution can occur after ILM disruption. These suggest that ILM may play an important role as a biomarker in the evolution of MTM.
CONUT Score Predicts Early Morbidity After Liver Transplantation: A Collaborative Study
Authors: Spoletini Gabriele , Ferri Flaminia , Mauro Alberto , Mennini Gianluca , Bianco Giuseppe , Cardinale Vincenzo , Agnes Salvatore , Rossi Massimo , Avolio Alfonso Wolfango , Lai Quirino 
Published: January 2022

Introduction: Liver transplantation (LT) is burdened by the risk of post-operative morbidity. Identifying patients at higher risk of developing complications can help allocate resources in the perioperative phase. Controlling Nutritional Status (CONUT) score, based on lymphocyte count, serum albumin, and cholesterol levels, has been applied to various surgical specialties, proving reliable in predicting complications and prognosis. Our study aims to investigate the role of the CONUT score in predicting the development of early complications (within 90 days) after LT.Methods: This is a retrospective analysis of 209 patients with a calculable CONUT score within 2 months before LT. The ability of the CONUT score to predict severe complications, defined as a Comprehensive Complication Index (CCI) ≥42.1, was examined. Inverse Probability Treatment Weighting was used to balance the study population against potential confounders.Results: Patients with a CCI ≥42.1 had higher CONUT score values (median: 7 vs. 5, P-value &lt; 0.0001). The CONUT score showed a good diagnostic ability regarding post-LT morbidity, with an AUC = 0.72 (95.0%CI = 0.64–0.79; P-value &lt; 0.0001). The CONUT score was the only independent risk factor identified for a complicated post-LT course, with an odds ratio = 1.39 (P-value &lt; 0.0001). The 90-day survival rate was 98.8% and 87.5% for patients with a CONUT score &lt;8 and ≥8, respectively.Conclusions: Pre-operative CONUT score is a helpful tool to identify patients at increased post-LT morbidity risk. Further refinements in the score composition, specific to the LT population, could be obtained with prospective studies.
Phase Ib Study of the Histone Deacetylase 6 Inhibitor Citarinostat in Combination With Paclitaxel in Patients With Advanced Solid Tumors
Authors: Gordon Michael S. , Shapiro Geoffrey I. , Sarantopoulos John , Juric Dejan , Lu Brian , Zarotiadou Angeliki , Connarn Jamie N. , Le Bruchec Yvan , Dumitru Calin Dan , Harvey R. Donald 
Published: January 2022

BackgroundCitarinostat (CC-96241; previously ACY-241), an oral inhibitor of histone deacetylases (HDACs) with selectivity for HDAC6, has demonstrated synergistic anticancer activity with paclitaxel in multiple solid tumor models. Combination therapy using citarinostat with paclitaxel was evaluated in this phase Ib 3 + 3 dose-escalation study in patients with advanced solid tumors.MethodsPatients with previously treated advanced solid tumors received citarinostat 180, 360, or 480 mg once daily on days 1 to 21 plus paclitaxel 80 mg/m2 on days 1, 8, and 15 of 28-day cycles until disease progression or unacceptable toxicity. The primary endpoint was determination of the maximum tolerated dose (MTD). Secondary endpoints included safety, antitumor activity, pharmacokinetics, and pharmacodynamics.ResultsTwenty patients were enrolled and received study treatment; 15 had received prior taxane therapy. No dose-limiting toxicities were reported at any dose; therefore, the MTD was not identified. Citarinostat 360 vs 480 mg was associated with reduced incidence and severity of neutropenia. Three patients experienced a confirmed partial response and 13 achieved stable disease. Pharmacokinetic parameters were linear up to citarinostat 360 mg, the dose at which the highest levels of histone and tubulin acetylation were observed in peripheral blood mononuclear cells.ConclusionsThe combination of citarinostat plus paclitaxel showed an acceptable safety profile, with no unexpected or dose-limiting toxicities and potential evidence of antitumor activity in patients with heavily pretreated advanced solid tumors. Citarinostat 360 mg once daily is considered the recommended phase II dose for use in combination with paclitaxel 80 mg/m2 every 3 of 4 weeks. This trial is registered on ClinicalTrials.gov (NCT02551185).