bluuebunny/crossref_metadata_2025_split huggingface dataset:
A strip‐based total dissolved solids sensor for water quality analysis
Authors: Makhdoumi Akram Mohammad , Ramezannezhad Mohammad , Nikfarjam Alireza , Kabiri Shima , Ehyaei Sana 
Published: January 2022

AbstractIn this study, a portable Total Dissolved Solids (TDS) and Electrical Conductivity (EC) of water sensor using a disposable polymer strip and the readout circuit segments is presented. The strip consists of gold electrodes and a vent hole for water flow injection inside the measurement chamber. This strip reduces the cost of the measurement, decreases the measurement duration, and increases the repeatability of the system. Besides, there is no demand for electrodes cleaning and recalibration of the system. According to the electrical model of strip electrodes and their frequency responses, the EC value is in the range of 78.1–1230.8 (µs)/cm. The experimental tests are carried out based on the frequency method in the concentration range within 50–800 ppm. The results confirm the linear relation of TDS concentration on the EC voltage value which is 1.9 mV for 1 ppm resolution. Also, the effect of temperature variations on the EC value is investigated. Furthermore, the average amount of error for EC measurement exhibits an acceptable amount in comparison with other studies. Moreover, studying the drift variations for 20 days reveals the maximum drift of 1.6%. Finally, according to the present study, the sensor is a suitable candidate for practical applications.
The Relationship between an Accessory Maxillary Ostium and Variations in Structures Adjacent to the Maxillary Sinus without Polyps
Authors: Serindere Gozde , Gunduz Kaan , Avsever Hakan 
Published: January 2022

Abstract
Introduction The maxillary sinus and its variations are very important to dentistry and rhinology.
Objective To investigate the effect of the accessory maxillary ostium (AMO) on the variations of adjacent structures of the maxillary sinus.
Methods The computed tomography (CT) images of 400 patients were retrospectively evaluated. The prevalence of AMO was calculated. The relationship between morphological variations of adjacent structures of maxillary sinus such as agger nasi cell (ANC), Haller cell (HC), nasal septum deviation (NSD), hypertrophy of inferior concha (HIC), pneumatization of middle concha (PMC), mucus retention cyst (MRC), mucosal thickening (MT), and maxillary sinusitis (MS), as well as the presence of AMO, were investigated.
Results Presence of AMO was diagnosed in 42 patients (10.5%), having been found in 4.5% of the patients only on the right side, in 1.25% of the patients only on the left side, and in 4.75% of the patients on both sides. There is an increasing incidence of ANC, HC, NSD, HIC, and PMC in the presence of AMO and MS. There is a decreasing incidence of MRC in the presence of AMO. Furthermore, AMO does not affect the incidence of MT.
Conclusion This study showed that most parameters, except for MRC and MT, had increasing incidence in the presence of AMO. It is important for radiologists and rhinologists to have knowledge about the location of AMO and the presence of variations of MS adjacent structures to avoid surgical complications.
Atomic force microscopy reveals distinct protofilament-scale structural dynamics in depolymerizing microtubule arrays
Authors: Wijeratne Sithara S. , Marchan Michelle F. , Tresback Jason S. , Subramanian Radhika 
Published: January 2022

Significance
One cannot help but marvel at the precise organization of microtubule polymers in cellular structures such as the axoneme and the spindle. However, our understanding of the biochemical mechanisms that sculpt these arrays comes largely from in vitro experiments with a small number (one or two) of microtubules. This is somewhat akin to studying the architecture of multilane highways by studying one-lane streets. Here, we directly visualize depolymerizing microtubule arrays at individual microtubule and protofilament resolution using atomic force microscopy. Our results reveal differences in microtubule depolymerase activity and provide insights into how these differences in enzymatic activity on the nanometer scale can result in the differential remodeling of multimicrotubule arrays on the micron-length scale.
Strong evidence for a weakly oxygenated ocean–atmosphere system during the Proterozoic
Authors: Wang Changle , Lechte Maxwell A. , Reinhard Christopher T. , Asael Dan , Cole Devon B. , Halverson Galen P. , Porter Susannah M. , Galili Nir , Halevy Itay , Rainbird Robert H. , Lyons Timothy W. , Planavsky Noah J. 
Published: January 2022

SignificanceEarth’s transition from anoxic oceans and atmosphere to a well-oxygenated state led to major changes in nearly every surficial system. However, estimates of surface oxygen levels in the billion years preceding this shift span two orders of magnitude, suggesting a poor understanding of the evolution of the oxygen cycle. We use the isotopic record of iron oxides deposited in ancient shallow marine environments to show that oxygen remained at extremely low levels in the ocean–atmosphere system for most of Earth’s history, and that a rise in oxygen occurred in step with the expansion of complex, eukaryotic ecosystems. These results indicate that Earth is capable of stabilizing at low atmospheric oxygen levels, with important implications for exploration of exoplanet biosignatures.
ER-phagy requires the assembly of actin at sites of contact between the cortical ER and endocytic pits
Authors: Liu Dongmei , Mari Muriel , Li Xia , Reggiori Fulvio , Ferro-Novick Susan , Novick Peter 
Published: January 2022

Significance
Portions of the endoplasmic reticulum (ER) are degraded by autophagy (ER-phagy) in response to starvation or the accumulation of misfolded proteins. We show that ER-phagy requires assembly of actin at sites of contact between the edges of ER sheets and endocytic pits on the plasma membrane. Actin assembly may help to bring an element of the ER carrying the selective autophagy receptor Atg40 into the cell interior, where it associates with Atg11, a scaffold needed to recruit components for autophagosome assembly. Understanding the mechanism by which regions of the ER are selected for degradation and sequestered within autophagosomes may help in the development of novel approaches to treat diseases that result from the accumulation of misfolded proteins within the ER.
Trends in surface equivalent potential temperature: A more comprehensive metric for global warming and weather extremes
Authors: Song Fengfei , Zhang Guang J. , Ramanathan V. , Leung L. Ruby 
Published: January 2022

SignificanceThe Earth has warmed by 1.2±0.1 °C since the preindustrial era. The most common metric to measure the ongoing global warming is surface air temperature since it has long and reliable observational records. However, surface air temperature alone does not fully describe the nature of global warming and its impact on climate and weather extremes. Here we show that surface equivalent potential temperature, which combines the surface air temperature and humidity, is a more comprehensive metric not only for the global warming but also for its impact on climate and weather extremes including tropical deep convection and extreme heat waves. We recommend that it should be used more widely in future climate change studies.
Literature and language education
Authors: Bobkina Jelena , Romero Elena Domínguez , Sastre-Merino Susana 
Published: January 2022

AbstractThis study explores the perceptions of English language teachers of the use of literature in the context of bilingual secondary education in the region of Madrid (Spain). An approach was adopted under which eighty-one English teacher participants completed a questionnaire so that a quantitative methodological approach of a descriptive – correlational nature could be adopted for this study. Results unveiled teacher perceptions of the benefits and drawbacks of using literary texts as a teaching and learning tool, their selection of literary texts and their actual implementation of these texts in their English classes, as well as the training needs for the effective use of literary texts as a language teaching and learning tool in the bilingual EFL secondary classroom. The pedagogical implications of the study emphasize the need to redesign and consolidate teacher training programmes containing literature and innovative teaching aspects both in initial teacher training programmes and for continued training.
Shock formation in magnetized plasma under the influence of polarization force and nonadiabaticity of dust charge variation
Authors: Bansal Sona , S Gill Tarsem 
Published: January 2022

Abstract
In this work, we have analyzed the impact of polarization force, angle of obliqueness under the influence of nonadiabatic dust charge fluctuation on shock waves formation in magneto rotating plasma. The present plasma model is consisting of negatively charged dust grains, Maxwellian electrons, nonextensive ions. The dissipation is introduced in the system via nonadiabaticity, a new mechanism for the formation of shock waves. Using standard reductive perturbation method, nonlinear equation namely Korteweg–de Vries Burgers equation is derived and solution is obtained using the Tanh method. It is shown that dust charge fluctuation is the main source of dissipation. We have studied the various parameteric influences on such shock structure and also showed how the gradual variations of these parameter affect the generation and structure of the shocks in their respective domain. Much of experiments on dusty plasma with nonadiabatic dust charge fluctuation will benefit from the parametric study.
Use of Anthropometric Indices to Identify Appropriate Cuff Shapes for Blood Pressure Measurement: Normative Data for Adults
Authors: Palatini Paolo , Fania Claudio , Ermolao Andrea , Battista Francesca , Saladini Francesca 
Published: January 2022

Abstract

Background
Published evidence has shown that troncoconical cuffs improve blood pressure measurement accuracy in subjects with obesity. However, the exact shape of these cuffs according to arm size is unknown. The aim of the present study was to investigate the anthropometric characteristics of the arm in a large population in order to identify the appropriate shape of the cuff for each arm.

Methods
The anthropometric characteristics were assessed in 729 adults with middle arm circumference ranging from 16 to 55 cm. Subjects were divided into classes of arm size and the upper-arm slant angle of the truncated cone was calculated.

Results
In the whole sample, the conical shape of the arm progressively increased with increasing arm circumference (r = 0.61, P &lt; 0.0001). Independent predictors of the conical shape were arm circumference (P &lt; 0.0001) and length (negative relationship, P &lt; 0.0001), and female sex (P = 0.048). Women had a slightly more pronounced conical shape of the arm (P = 0.007) than men. However, the decrease in the slant angle across the arm-size classes showed a similar trend in men and women, and in a 2-way analysis of variance there was no interactive effect between arm size and sex on the conical shape of the arm (P = 0.11). On the basis of the slant angle calculated in each arm-size class, we provide dimensions for bladders that would appropriately fit the arm within a given circumference range.

Conclusions
To properly fit the upper arm in patients with obesity cuffs should have a troncoconical shape. This study provides reference data according to arm size.
Fast and compact matching statistics analytics
Authors: Cunial Fabio , Denas Olgert , Belazzougui Djamal 
Published: January 2022

AbstractMotivationFast, lightweight methods for comparing the sequence of ever larger assembled genomes from ever growing databases are increasingly needed in the era of accurate long reads and pan-genome initiatives. Matching statistics is a popular method for computing whole-genome phylogenies and for detecting structural rearrangements between two genomes, since it is amenable to fast implementations that require a minimal setup of data structures. However, current implementations use a single core, take too much memory to represent the result, and do not provide efficient ways to analyze the output in order to explore local similarities between the sequences.ResultsWe develop practical tools for computing matching statistics between large-scale strings, and for analyzing its values, faster and using less memory than the state-of-the-art. Specifically, we design a parallel algorithm for shared-memory machines that computes matching statistics 30 times faster with 48 cores in the cases that are most difficult to parallelize. We design a lossy compression scheme that shrinks the matching statistics array to a bitvector that takes from 0.8 to 0.2 bits per character, depending on the dataset and on the value of a threshold, and that achieves 0.04 bits per character in some variants. And we provide efficient implementations of range-maximum and range-sum queries that take a few tens of milliseconds while operating on our compact representations, and that allow computing key local statistics about the similarity between two strings. Our toolkit makes construction, storage and analysis of matching statistics arrays practical for multiple pairs of the largest genomes available today, possibly enabling new applications in comparative genomics.Availability and implementationOur C/C++ code is available at https://github.com/odenas/indexed_ms under GPL-3.0. The data underlying this article are available in NCBI Genome at https://www.ncbi.nlm.nih.gov/genome and in the International Genome Sample Resource (IGSR) at https://www.internationalgenome.org.Supplementary informationSupplementary data are available at Bioinformatics online.
Landscape genetics reveal low diversity and adaptive divergence inPortulaca hatschbachii(Portulacaceae): an endangered species endemic to rocky outcrops of the Atlantic Forest
Authors: Feliciano Daniele Cassiano , De Godoy Sara Mataroli , Da Silva João Fernando Marques , Góes Bruna Delgado , Ferraz José Roberto , De Oliveira Santos Patrícia , Da Silva Ribeiro José Eduardo Lahoz , Ruas Paulo Maurício , De Fátima Ruas Claudete 
Published: January 2022

AbstractPortulaca hatschbachii is endemic to the basaltic rocky outcrops that are distributed, in a discontinuous way, along the Third Plateau of Paraná State, Brazil, composing environments that form the Subtropical Highland Grasslands of the Atlantic Forest Biome. Considering the risk of extinction of the species and the massive anthropization of these outcrops, we applied AFLP, ITS and rps16 molecular markers in ten populations throughout the area of occurrence of the species to generate information about the genetic status of P. hatschbachii and contribute to the development of conservation strategies. Low rates of genetic diversity, high population structure, restricted gene flow and the presence of diversifying selection were observed for the populations. The analysis of variation partitioning (R2adj. = 63.60%) showed that environmental variables have a greater influence on the distribution of variation of loci under selection (R2adj. = 26.70%) than geographical isolation (R2adj. = 1.20%). The strong population structure, for both neutral and selected loci, suggests an isolation by adaptation mechanism (IBA) occurring in populations and highlights the need and urgency for in situ conservation plans for the species and its occurrence on rocky outcrops.
Stopping kidney protection in the elderly following acute kidney injury: think mortality
Authors: Carriazo Sol , Ortiz Alberto 
Published: January 2022

ABSTRACTChronic kidney disease (CKD) is projected to become the fifth most common global cause of death by 2040. This illustrates a key consequence of CKD, i.e. premature mortality. Since nephroprotective drugs such as renin–angiotensin system (RAS) blockers and sodium–glucose transport protein 2 (SGLT2) inhibitors decrease glomerular hyperfiltration, they may be stopped following an episode of acute kidney injury (AKI). This may theoretically modify the risks of subsequent events, ranging from hyperkalaemia to CKD progression to cardiovascular events, but the evidence so far has been inconsistent. Roemer et al. have now addressed the shortcomings of prior studies. In a population of mostly elderly (median age 78 years) prevalent users of RAS blockers with an indication for this therapy and who survived for at least 3 months after discharge following a hospitalization characterized by moderate to severe AKI, roughly 50% had stopped RAS blockade at 3 months. Stopping RAS blockade was associated with an increased risk of a primary composite outcome of death, myocardial infarction and stroke, of which a large majority (80%) of events were deaths. In contrast, the risk of hyperkalaemia was reduced and the risk of repeated AKI, CKD progression or heart failure hospitalization was unchanged in patients who stopped RAS blockers. These findings call for a re-evaluation of the practice of stopping RAS blockers in the long-term following AKI and suggest that studies are needed regarding similar practices for SGLT2 inhibitors.
Unexplained syncope in a young athlete: the diagnostic process to find the diagnosis—a case report
Authors: Bergau Leonard , Sohns Christian , Sommer Philipp 
Published: January 2022

Abstract

Background
Differential diagnosis of recurrent syncope is a routine procedure in clinical practice. Most of these syncopes are benign reflex syncopes but identifying patients with cardiac syncope is crucial to prevent fatal outcomes.

Case summary
In this case report, we present the case of a young athlete with recurrent unexplained syncope referred to us for a second opinion. Despite normal resting-electrocardiography and echocardiography, he developed frequent polymorphic and bidirectional premature ventricular contractions during exercise. Genetic testing confirmed a mutation in the RyR2-gene and the diagnosis of catecholaminergic polymorphic ventricular tachycardia was made. A medical therapy with betablockers was initiated but poorly tolerated, so that an implantable cardioverter-defibrillator was implanted. Furthermore, family screening revealed his mother and his sister to be genetic carriers as well. Implantable cardioverter-defibrillator implantation was performed in both family members. The patient did not experience any syncope or arrhythmic episodes during the follow-up period.

Discussion
This case report highlights the importance of thorough diagnostic and potential pitfalls in patients with unexplained syncope. Sometimes, the diagnostic steps need to be extended or repeated to detect rare or potential malignant causes of syncope.
A large deletion at the cortex locus eliminates butterfly wing patterning
Authors: Hanly Joseph J , Livraghi Luca , Heryanto Christa , McMillan W Owen , Jiggins Chris D , Gilbert Lawrence E , Martin Arnaud 
Published: January 2022

AbstractAs the genetic basis of natural and domesticated variation has been described in recent years, a number of hotspot genes have been repeatedly identified as the targets of selection, Heliconius butterflies display a spectacular diversity of pattern variants in the wild and the genetic basis of these patterns has been well-described. Here, we sought to identify the mechanism behind an unusual pattern variant that is instead found in captivity, the ivory mutant, in which all scales on both the wings and body become white or yellow. Using a combination of autozygosity mapping and coverage analysis from 37 captive individuals, we identify a 78-kb deletion at the cortex wing patterning locus, a gene which has been associated with wing pattern evolution in H. melpomene and 10 divergent lepidopteran species. This deletion is undetected among 458 wild Heliconius genomes samples, and its dosage explains both homozygous and heterozygous ivory phenotypes found in captivity. The deletion spans a large 5′ region of the cortex gene that includes a facultative 5′UTR exon detected in larval wing disk transcriptomes. CRISPR mutagenesis of this exon replicates the wing phenotypes from coding knock-outs of cortex, consistent with a functional role of ivory-deleted elements in establishing scale color fate. Population demographics reveal that the stock giving rise to the ivory mutant has a mixed origin from across the wild range of H. melpomene, and supports a scenario where the ivory mutation occurred after the introduction of cortex haplotypes from Ecuador. Homozygotes for the ivory deletion are inviable while heterozygotes are the targets of artificial selection, joining 40 other examples of allelic variants that provide heterozygous advantage in animal populations under artificial selection by fanciers and breeders. Finally, our results highlight the promise of autozygosity and association mapping for identifying the genetic basis of aberrant mutations in captive insect populations.
High accuracy of genome-enabled prediction of belowground and physiological traits in barley seedlings
Authors: Puglisi Damiano , Visioni Andrea , Ozkan Hakan , Kara İbrahim , Lo Piero Angela Roberta , Rachdad Fatima Ezzahra , Tondelli Alessandro , Valè Giampiero , Cattivelli Luigi , Fricano Agostino 
Published: January 2022

Abstract
In plants, the study of belowground traits is gaining momentum due to their importance on yield formation and the uptake of water and nutrients. In several cereal crops, seminal root number and seminal root angle are proxy traits of the root system architecture at the mature stages, which in turn contributes to modulating the uptake of water and nutrients. Along with seminal root number and seminal root angle, experimental evidence indicates that the transpiration rate response to evaporative demand or vapor pressure deficit is a key physiological trait that might be targeted to cope with drought tolerance as the reduction of the water flux to leaves for limiting transpiration rate at high levels of vapor pressure deficit allows to better manage soil moisture. In the present study, we examined the phenotypic diversity of seminal root number, seminal root angle, and transpiration rate at the seedling stage in a panel of 8-way Multiparent Advanced Generation Inter-Crosses lines of winter barley and correlated these traits with grain yield measured in different site-by-season combinations. Second, phenotypic and genotypic data of the Multiparent Advanced Generation Inter-Crosses population were combined to fit and cross-validate different genomic prediction models for these belowground and physiological traits. Genomic prediction models for seminal root number were fitted using threshold and log-normal models, considering these data as ordinal discrete variable and as count data, respectively, while for seminal root angle and transpiration rate, genomic prediction was implemented using models based on extended genomic best linear unbiased predictors. The results presented in this study show that genome-enabled prediction models of seminal root number, seminal root angle, and transpiration rate data have high predictive ability and that the best models investigated in the present study include first-order additive × additive epistatic interaction effects. Our analyses indicate that beyond grain yield, genomic prediction models might be used to predict belowground and physiological traits and pave the way to practical applications for barley improvement.