bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐term follow‐up findings in a Turkish girl with osteogenesis imperfecta type XX caused by a homozygous MESD variant
Authors: Uludağ Alkaya Dilek , Uyguner Zehra Oya , Güneş Nilay , Tüysüz Beyhan 
Published: January 2022

AbstractOsteogenesis imperfecta (OI) is a heterogeneous group of disorders with bone fragility. In 2019, homozygous pathogenic variants in MESD were described for the first time in five patients with severe form of OI. To date, 12 patients have been reported. The aim of this study is to report long‐term follow‐up findings of a girl with MESD variant. She had triangular face, sparse hair, wide fontanelle, blue sclera, softening of the occipital bone, congenital torticollis, and long fingers. Wormian bones, multiple rib and long bone fractures, and platyspondyly were detected in her skeletal radiographs. During the 21‐years follow‐up, intellectual disability, oligodontia, recurrent fractures, bowing of humerus, hip and knee contractures leading to crossing of the legs, swelling of the interphalangeal joints, and kyphoscoliosis were observed. Although the bisphosphonate treatment was started at 2.5 years of age, recurrent fractures continued to occur until 13 years of age. She lost her walking ability at 4.5 years of age. The final adult height was 128 cm (−6.0 SD). Homozygous c.631_632delAA (p.Lys211Glufs*19) variant in MESD was detected at 19 years of age. In conclusion, this study provides long‐term clinical and radiological findings in a patient with a very rare type of OI.
Ruthenium(II)‐Catalyzed C−H Alkenylation of 1,8‐Naphthalimide with Cyclic Imide as a Weakly Coordinating Directing Group
Authors: Thakur Rekha , Singh Iqubal , Paul Kamaldeep 
Published: January 2022

AbstractRuthenium‐catalyzed direct C−H alkenylation of 1,8‐naphthalimide has been explored using cyclic imide as a weak directing group and successfully introduce alkenyl group(s) into 1,8‐naphthalimide skeleton at the inactive C2 and/or C7 position(s). The aromatic ring of 1,8‐naphthalimide is substituted with electron‐withdrawing and ‐donating groups, and N‐alkyl/aryl groups on cyclic imide are compatible in these coupling reactions.
Habitat use by the island lemurs of Nosy Be, Madagascar
Authors: Tinsman Jen , Volampeno Sylviane , Ganas‐Swaray Jessica , Gann Daniel , Andrianirina Natacha , Chamizo Madison , Ralazampirenena Claude , Ranaivoarisoa Jean F. , Ravaoarisoa Hasina , Rivero Josie , Zamora Andrew , Gomes Cristina M. 
Published: January 2022

AbstractMadagascar's lemurs are threatened by forest loss, fragmentation, and degradation. Many species use flexible behaviors to survive in degraded habitat, but their ability to persist in very small areas may be limited. Insular lemurs, like those found on Nosy Be, an island off the northwestern coast of Madagascar, are at heightened risk of sudden population declines and extirpation. Nosy Be is home to two Critically Endangered species—the endemic Nosy Be sportive lemur (Lepilemur tymerlachsoni) and Claire's mouse lemur (Microcebus mamiratra)—as well as the Endangered black lemur (Eulemur macaco). Most of the remaining forest on Nosy Be is protected by the 862‐ha Lokobe National Park. To document how Nosy Be lemurs use their restricted habitat, we conducted vegetation and reconnaissance surveys on 53 transects in and around Lokobe. We collected data on tree size, canopy cover, understory visibility, and elevation for 248 lemur sightings. We used a spatially explicit, multi‐species occupancy model to investigate which forest‐structure variables are important to lemurs. Our results represent some of the first data on habitat use by insular lemurs. Black lemurs preferred significantly larger trees and areas with less dense understory. They also occurred significantly less outside of Lokobe National Park, even when accounting for sampling effort and geography. The distributions of the sportive and mouse lemurs were not related to the forest structure variables we documented, but they did negatively predict each other—perhaps because their habitat requirements differ. These results also underscore the importance of the national park to protecting the black lemur population on Nosy Be and raise questions about what factors do influence the distribution of Nosy Be's smaller lemurs. Close monitoring is needed to prevent these populations and the ecosystem services they provide from disappearing, as have other island lemurs.
Yawn contagion in bonobos: Another group, another story
Authors: Norscia Ivan , Caselli Marta , De Meo Gabriele , Cordoni Giada , Guéry Jean‐Pascal , Demuru Elisa 
Published: January 2022

AbstractIn primates, yawn contagion (the yawning response elicited by others' yawn) is variably influenced by individual (e.g., sex, age) and social factors (e.g., familiarity) and possibly linked to interindividual synchronization, coordination, and emotional contagion. Two out of three studies on yawn contagion in bonobos (Pan paniscus), found the presence of the phenomenon with mixed results concerning the effect of familiarity and no replication on its modulating factors. To address this puzzling issue, we recorded all occurrences data on yawn contagion in a captive bonobo group (March–June 2021; 18 individuals; La Vallée des Singes, France). Contrary to chimpanzees and humans, the number of triggering yawns increased contagion, possibly owing to a higher stimulus threshold. This aspect may explain the interindividual variability observed in yawn contagion rates. In subjects under weaning, we did not detect yawn contagion and, as it occurs in certain human cohorts, yawn contagion declined with age, possibly due to reduced sensitivity to others. Females responded more than males and elicited more responses from females when showing sexual swelling. As reproductive females are central in bonobo society, our results support the hypothesis that—as in other Hominini—the most influential sex can influence yawn contagion. The relationship quality (measured via grooming/play) did not affect yawn contagion, possibly due to bonobos' xenophilic nature. Overall, this study confirms the presence of yawn contagion in bonobos and introduces new elements on its modulating factors, pointing toward the necessity of cross‐group studies.
Structure Elucidation and Computationally Guided Synthesis of SSZ‐43: A One‐Dimensional 12‐Ring Zeolite with Unique Sinusoidal Channels
Authors: Roslova Maria , Cybulskis Viktor J. , Davis Mark E. , Zones Stacey I. , Zou Xiaodong , Xie Dan 
Published: January 2022

AbstractThe structure of zeolite SSZ‐43 was determined by 3D electron diffraction, synchrotron X‐ray powder diffraction, and high‐resolution transmission electron microscopy. The SSZ‐43 framework forms one‐dimensional, sinusoidal 12‐ring channels from 5461 butterfly units commonly found in other zeolites, but with unique 6.5×6.5 Å apertures and 12‐ring 6.5×8.9 Å windows perpendicular to the channels. SSZ‐43 crystals are intergrowths of two polytypes: ≈90 % orthorhombic polytype A with ABAB stacking of the 12‐rings, and ≈10 % monoclinic polytype B with ABCABC stacking. Molecular modeling performed on the idealized Si‐SSZ‐43 structure along with empirical relationships for zeolite selectivity in boron‐ and aluminum‐containing synthesis gels were used in a combined approach to design new di‐quaternary ammonium organic structure‐directing agents (OSDAs). Experimental trials demonstrated that the new OSDAs produced SSZ‐43 over a broader range of compositions than previous mono‐quaternary OSDAs.
Simultaneous Control of Pore‐Space Partition and Charge Distribution in Multi‐Modular Metal–Organic Frameworks
Authors: Hong Anh N. , Kusumoputro Emily , Wang Yanxiang , Yang Huajun , Chen Yichong , Bu Xianhui , Feng Pingyun 
Published: January 2022

AbstractWe report here a strategy for making anionic pacs type porous materials by combining pore space partition with charge reallocation. The method uses the first negatively charged pore partition ligand (2,5,8‐tri‐(4‐pyridyl)‐1,3,4,6,7,9‐hexaazaphenalene, H‐tph) that simultaneously enables pore partition and charge reallocation. Over two dozen anionic pacs materials have been made to demonstrate their excellent chemical stability and a high degree of tunability. Notably, Ni3‐bdt‐tph (bdt=1,4‐benzeneditetrazolate) exhibits month‐long water stability, while CoV‐bdt‐tph sets a new benchmark for C2H2 storage capacity under ambient conditions for ionic MOFs. In addition to tunable in‐framework modules, we show feasibility to tune the type and concentration of extra‐framework counter cations and their influence on both stability and capability to separate industrial C3H8/C3H6 and C6H6/C6H12 mixtures.
Finite Assembly of Three‐Dimensional DNA Hierarchical Nanoarchitectures through Orthogonal and Directional Bonding
Authors: Zhou Yihao , Dong Jinyi , Zhou Chao , Wang Qiangbin 
Published: January 2022

AbstractReliable orthogonal bonding with precise and flexible orientation control would be ideal for building finite complex nanostructures via self‐assembly. Employing a three‐dimensional (3D) DNA origami, hexagonal prism DNA origami (HDO), as building block, we demonstrate it is practical to construct finite hierarchical nanoarchitectures with complicated conformations through orthogonal and directional bonding. The as‐designed HDO building block has twelve prescribed directional valences in 3D space and each of them supports two opposite orientations, yielding the capability to generate abundant directional bonding. Meanwhile, we minimize the thorny non‐specific interactions among HDOs and enable the orthogonal bonding between any two valences based on self‐similar designing. Consequently, various hierarchical nanostructures are prepared at will simply by the combination of HDOs with appropriate valences. We believe this route towards hierarchically assembly is inspiring and hope it will facilitate the fabrication of functional superstructures.
One‐Step Synthesis of Degradable Vinylic Polymer‐Based Latexes via Aqueous Radical Emulsion Polymerization
Authors: Galanopoulo Paul , Gil Noémie , Gigmes Didier , Lefay Catherine , Guillaneuf Yohann , Lages Maëlle , Nicolas Julien , Lansalot Muriel , D'Agosto Franck 
Published: January 2022

AbstractAqueous emulsion copolymerizations of dibenzo[c,e]oxepane‐5‐thione (DOT) were performed with n‐butyl acrylate (BA), styrene (S) and a combination of both. In all cases, stable latexes were obtained in less than two hours under conventional conditions; that is in the presence of sodium dodecyl sulfate (SDS) used as surfactant and potassium persulfate (KPS) as initiator. A limited solubility of DOT in BA was observed compared to S, yielding to a more homogeneous integration of DOT units in the PS latex. In both cases, the copolymer could be easily degraded under basic conditions. Emulsion terpolymerization between DOT, BA and S allowed us to produce stable latexes not only composed of degradable chains but also featuring a broad range of glass transition temperatures.
Noninvasively ventilated and easily operated mouse model of myocardial infarction
Authors: He Yuan , Lu Xinlin , Liang Zheng , Chen Tao , Chen Can , Zhang Yuanqi , Lei Wei 
Published: January 2022

AbstractThe laboratory mouse is an animal model commonly used in cardiovascular research; however, it has technical challenges as a model for myocardial infarction (MI). In this study, a novel noninvasively ventilated and easily operated experimental mouse model of MI was established in Kunming mice based on a left anterior descending (LAD) coronary artery rupture. Overall, 95% of sham mice and 84% of mice with a ruptured LAD survived the surgery. The results of 2,3,5‐triphenyl‐2H‐tetrazolium chloride and hematoxylin–eosin staining showed that obvious infarcts formed after LAD rupture. ST‐segment elevation or depression emerging in the electrocardiogram of the novel MI model indicated a myocardial ischemic injury. Reduced cardiac contractility and increased cardiac troponin I and creatine kinase‐MB after LAD‐rupture implied myocardial necrosis. Furthermore, the serum levels of IL‐1β and IL‐6 were significantly upregulated after LAD‐rupture. Overall, the LAD‐rupture method, utilizing noninvasive ventilation, was a reliable and easily‐performed model of MI in mice.
Absolute configuration of cytotoxic anthraquinones from a Brazilian cave soil‐derived fungus, Aspergillus sp. SDC28
Authors: Gubiani Juliana R. , Bernardi Darlon I. , De Paula Caio C. P. , Seleghim Mirna H. R. , Ferreira Antonio G. , Batista Andrea N. L. , Batista  João M. , Oliveira Lucianne F. P. , Lira Simone P. , Burdette Joanna E. , Berlinck Roberto G. S. 
Published: January 2022

AbstractMicrobial strains isolated from extreme and understudied environments, such as caves, are still poorly investigated for the production of bioactive secondary metabolites. Investigation of the ethyl acetate extract from the growth medium produced by the soil‐derived fungus Aspergillus sp. SDC28, isolated from a Brazilian cave, yielded two anthraquinones: versicolorin C (1) and versiconol (2). The complete assignment of nuclear magnetic resonance and mass spectroscopic data of 1 and 2 was performed for the first time. Moreover, the yet unreported absolute configuration of both compounds was unambiguously established by analysis of experimental and theoretical electronic circular dichroism data. Vibrational circular dichroism was also applied to confirm the absolute stereochemistry of 2. Compounds 1 and 2 showed cytotoxic activity against human ovarian cancer cells (OVCAR3).
Medium optimization and subsequent fermentative regulation enabled the scaled‐up production of anti‐tuberculosis drug leads ilamycin‐E1/E2
Authors: Fan Zhiying , Tong Nian , Zhuang Zhoukang , Ma Cheng , Ma Junying , Ju Jianhua , Duan Yanwen , Zhu Xiangcheng 
Published: January 2022

AbstractBackgroundTuberculosis (TB) and its evolving drug resistance have exerted severe threats on the global health, hence it is still essential to develop novel anti‐TB antibiotics. Ilamycin‐E1/E2 is a pair of cycloheptapeptide enantiomers obtained from a marine Streptomyces atratus SCSIO ZH16‐ΔilaR mutant, and has presented significant anti‐TB activities as promising drug lead compounds, but their clinical development has been hampered by low fermentation titers.Main methods and major resultsBy applying the statistical Plackett–Burman design (PBD) model, bacterial peptone was first screened out as the only significant but negative factor to affect the ilamycin‐E1/E2 production. Subsequent single factor optimization in shaking flasks revealed that the replacement of bacterial peptone with malt extract could not only eliminate the accumulation of porphyrin‐type competitive byproducts but also improve the titer of ilamycin‐E1/E2 from original 13.6 ± 0.8 to 142.7 ± 5.7 mg L−1, about 10.5‐fold increase. Next, a pH coordinated feeding strategy was adopted in 30 L fermentor and obtained 169.8 ± 2.5 mg L−1 ilamycin‐E1/E2, but further scaled‐up production in 300 L fermentor only gave a titer of 131.5 ± 7.5 mg L−1 due to the unsynchronization of feeding response and pH change. Consequently, a continuous pulse feeding strategy was utilized in 300 L fermentor to solve the above problem and finally achieved 415.7 ± 29.2 mg L−1 ilamycin‐E1/E2, representing a 30.5‐fold improvement.ImplicationOur work has provided a solid basis to acquire sufficient ilamycin‐E1/E2 lead compounds and then support their potential anti‐TB drug development.
Cell‐derived and enzyme‐based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial
Authors: Nellinger Svenja , Mrsic Ivana , Keller Silke , Heine Simon , Southan Alexander , Bach Monika , Volz Ann‐Cathrin , Chassé Thomas , Kluger Petra J. 
Published: January 2022

AbstractDue to its availability and minimal invasive harvesting human adipose tissue‐derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell‐derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively toward their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose‐derived stem cells, toward their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans compared with cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM‐based biomaterial for their specific application and the interpretation of obtained results.
Enhancing biosynthesis of 2'‐Fucosyllactose in Escherichia coli through engineering lactose operon for lactose transport and α ‐1,2‐Fucosyltransferase for solubility
Authors: Park Bum Seok , Choi Yun Hee , Kim Min Woo , Park Beom Gi , Kim Eun‐Jung , Kim Jin Young , Kim Jung Hwa , Kim Byung‐Gee 
Published: January 2022

Abstract2'‐Fucosyllactose (2'‐FL) is the most abundant oligosaccharide in human milk and one of the most actively studied human milk oligosaccharides (HMOs). When 2'‐FL is produced through biological production using a microorganism, like Escherichia coli, d‐lactose is often externally fed as an acceptor substrate for fucosyltransferase (FT). When d‐glucose is used as a carbon source for the cell growth and d‐lactose is transported by lactose permease (LacY) in lac operon, d‐lactose transport is under the control of catabolite repression (CR), limiting the supply of d‐lactose for FT reaction in the cell, hence decreasing the production of 2'‐FL. In this study, a remarkable increase of 2'‐FL production was achieved by relieving the CR from the lac operon of the host E. coli BL21 and introducing adequate site‐specific mutations into α‐1,2‐FT (FutC) for enhancement of catalytic activity and solubility. For the host engineering, the native lac promoter (Plac) was substituted for tac promoter (Ptac), so that the lac operon could be turned on, but not subjected to CR by high d‐glucose concentration. Next, for protein engineering of FutC, family multiple sequence analysis for conserved amino acid sequences and protein–ligand substrate docking analysis led us to find several mutation sites, which could increase the solubility of FutC and its activity. As a result, a combination of four mutation sites (F40S/Q150H/C151R/Q239S) was identified as the best candidate, and the quadruple mutant of FutC enhanced 2'‐FL titer by 2.4‐fold. When the above‐mentioned E. coli mutant host transformed with the quadruple mutant of futC was subjected to fed‐batch culture, 40 g l−1 of 2'‐FL titer was achieved with the productivity of 0.55 g l−1 h−1 and the specific 2'‐FL yield of 1.0 g g−1 dry cell weight.
The collaborative and contested interplay between business and civil society in circular economy transitions
Authors: Ho Chia‐Hao , Böhm Steffen , Monciardini David 
Published: January 2022

AbstractWe know from the corporate social responsibility (CSR) literature that civil society organizations (CSOs) play a crucial role in helping businesses transition towards more responsible and sustainable business models. Yet, in the circular economy (CE) field, such understanding is still scarce. This article develops a theoretical framework for understanding the dynamic interplay between business and civil society in CE transitions, understanding their mechanisms and strategies for cooperation and contestation. We develop the concept of ‘CE boundary work’ to understand how CSOs interact with firms to bring about CE innovation, outlining three ideal types: campaign based, resource efficiency based and circular design based. This contributes to our understanding of how CE transitions can be brought about effectively through cross‐sectoral interplays between civil society and business.
Bi/Zn Dual Single‐Atom Catalysts for Electroreduction of CO2 to Syngas
Authors: Meng Lingzhe , Zhang Erhuan , Peng Haoyu , Wang Yu , Wang Dingsheng , Rong Hongpan , Zhang Jiatao 
Published: January 2022

AbstractThe electrochemical CO2 reduction reaction to produce CO is one of the most promising pathways to eliminate CO2 emissions and store intermittent energy sources. However, the most critical usage of CO is via mixing with H2 to form syngas, which is a crucial feedstock for many value‐added chemicals. Therefore, producing syngas with a suitable CO/H2 ratio in a one‐step reaction is desirable in the CO2RR process. To achieve this end, dual single‐atom sites supported by the nitrogen‐doped carbon show great potential. Herein, we show that a dual single‐atom catalyst with Bi−N4 and Zn−N4 units is efficient for generating syngas with tunable CO/H2 ratios (0.20 to 2.92), which is of great significance to downstream industrial production. Moreover, this work highlights the potential to control the CO/H2 ratios for efficient syngas production using the coexisting single‐atom sites.
Rh(II)‐catalyzed Intermolecular Benzylic C(sp3)−H Alkylation of Methyl‐substituted Arenes by N‐Aryl‐α‐diazo‐β‐amidoesters
Authors: Yuan Shaoren , McLaren Eric J. , France Stefan 
Published: January 2022

AbstractHerein, a general approach to intermolecular benzylic C(sp3)−H alkylation of methyl‐substituted arenes is reported using metal carbenes derived from N‐aryl‐α‐diazo‐β‐amidoesters and dirhodium catalysts. Alkylated products were formed in up to 81 % yield with demonstrated functional group tolerance, outpacing previous literature. The unique amide‐ester scaffolding can be exploited through various derivatizations for broad synthetic utility and provides a starting point for the development of selectivity rules and reactivity profiles for these intermolecular C(sp3)−H functionalizations.
Influence of the MgO grade in MgO‐C refractory material and steel melt temperature on the inclusion population in Al‐treated steel
Authors: Kerber Florian , Malczyk Piotr , Stein Volker , Schemmel Thomas , Jansen Helge , Aneziris Christos G. 
Published: January 2022

AbstractThe influence of two industrially fabricated MgO‐C specimens differing in the MgO grade of the used MgO raw material on the population of nonmetallic inclusions via immersion tests was investigated. The immersion tests were performed in molten, Al‐treated 42CrMo4 steel at 1600°C and 1680°C in a steel casting simulator for 30 min. The inclusion population remaining within the solidified steel was characterized by the help of an EDS‐aided automated scanning electron microscope using an automated feature analysis. Several thousands of inclusions were detected and fully classified considering both their size and their elemental composition.The presented work reveals the impact of the MgO grade of the immersed MgO‐C specimens on the characteristics of the remaining nonmetallic inclusion population in dependency on the temperature of the molten steel. Significant differences in the total number of remaining inclusions and in regards to their size and species were exhibited and in‐depth analyzed.The paper contributes to the understanding of inclusion evolution mechanisms and propose potentially occurring phenomena based on the gained results.