bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nocturnal blood pressure rather than night‐to‐day blood pressure ratio is related to arterial stiffening in untreated young and middle‐aged adults with non‐dipper hypertension
Authors: Liu Jing , Su Xiaofeng , Nie Ying , Zeng Zhihuan , Chen Hongyan ,   the NARRAS investigators
Published: July 2022

AbstractLittle is known about nocturnal blood pressure (BP) or night‐to‐day BP ratio, which is a more specific determinant of arterial stiffness in subjects with non‐dipper hypertension? This study aims to investigate the correlation of nocturnal BP and brachial‐ankle pulse wave velocity (ba PWV), an index of arterial stiffness in untreated young and middle‐aged adults with non‐dipper hypertension.A cross‐sectional analysis of baseline parameters of the NARRAS trial was performed. Twenty‐four hour ambulatory BP measurements, ba PWV and routine clinical data collection were performed in all patients. The relationship of 24‐h ambulatory BP profiles, biochemical measures as well as demographic parameters and ba PWV were analyzed using Pearson's correlation and multiple stepwise regression analysis.A total of 77 patients (mean age 47.0 ± 11.7 years) with non‐dipper hypertension were included. Age, height, weight and nocturnal systolic BP were related to ba PWV in Pearson's correlation analysis. In stepwise regression analysis, age (β = 10.57, 95% confidence interval (CI): 6.099–15.042, p < 0.001) and weight (β = −3.835, 95% CI: −7.658‐−0.013, p = 0.049) are related to ba PWV. Nocturnal systolic BP (β = 8.662, 95% CI: 2.511–14.814, p = 0.006) was the independent predictors of ba PWV, even after night‐to‐day systolic BP ratio or 24‐h ambulatory BP profile were taken into account.Nocturnal systolic BP rather than night‐to‐day systolic BP ratio appears to be a more specific determinant for arterial stiffness, as assessed by ba PWV in young and middle‐aged adults with non‐dipper hypertension. 24‐h ambulatory BP measurements are essential for cardiovascular risk evaluation.
The positive correlation between brachial‐ankle pulse wave velocity and aortic diameter in Chinese patients with diabetes
Authors: Li Liping , Xie Wenhui , Li Qingqing , Hong Huashan 
Published: July 2022

AbstractAortic dilation is associated with an increased risk of cardiovascular diseases. Increased brachial‐ankle pulse wave velocity (baPWV) is a hallmark of vascular aging and arterial stiffness, as well as an important risk factor for vascular disease. This study aimed to retrospectively analyze the correlation between baPWV and aortic diameter (AoD) of inpatients with diabetes. A total of 1294 diabetic patients with the detailed medical records were investigated. Arterial stiffness was assessed using baPWV and AoD using echocardiography. The results showed that baPWV and AoD increase with age (p <0.05). Based on multiple linear regression analysis, age, systolic and diastolic blood pressure, left atrial diameter, right ventricle diameter, pulmonary artery diameter, peak velocity of early transmitral blood flow/peak velocity of late transmitral blood flow, and baPWV independently correlated with AoD in patients with diabetes. Additionally, an increased risk of aortic dilation occurred in the highest baPWV quartile compared with the lowest quartile (p <0.001). In conclusion, baPWV is independently and positively associated with AoD. Hence, prospective cohorts or randomized clinical trials will be the next step to further determine whether interventions designed to improve arterial stiffness in patients with diabetes will reduce the risk of aortic dilation.
Christian Europe Redux
Authors: Wolkenstein Fabio 
Published: July 2022

AbstractThe notion of ‘Christian Europe’ has returned with a vengeance in recent times. It figures prominently in the political rhetoric of conservative nationalists, who link appeals to Europe's Christian heritage and identity to avowedly illiberal political projects. This article examines this revived idea of Christian Europe by contrasting it with the meanings that prominent Christian Democratic leaders ascribed to the term in the post‐war era. This contrast is insightful because it reveals what is distinctive about present understandings of Christian Europe, and it is politically relevant because some of the most committed contemporary proponents of Christian Europe claim to be ‘true’ Christian Democrats. Using Ernst‐Wolfgang Böckenförde's work on the emergence of the modern state as a broad analytical frame, I show that today's visions of Christian Europe are more modern, statist and secular than their post‐war counterparts.
Synchronous primary cutaneous melanomas: a descriptive study of their clinical features, histology, genetic background of the patients and clinical outcomes
Authors: Antúnez‐Lay A. , Podlipnik S. , Carrera C. , Potrony M. , Tell‐Martí G. , Badenas C. , Puig‐Butille J.A. , Espinosa N. , Puig S. , Malvehy J. 
Published: July 2022

AbstractBackgroundAround 0.5% of cutaneous melanoma (CM) patients will present with synchronous melanomas when first seen. Moreover, 26–40% of patients with multiple primary melanomas present with synchronous lesions.ObjectivesTo assess the prevalence, clinical and histopathological characteristics, germline mutations and outcome in patients with synchronous melanoma.MethodsClinical and histopathological data from 4703 melanoma patients were included. Clinical, histological and genetic mutational status information was analysed. Kaplan–Meier curves were used to investigate survival outcomes.ResultsA total of 144 patients (3.06%) presented simultaneously with two or more primary melanomas. During follow‐up, 25.7% of patients with synchronous melanoma developed a new primary melanoma compared to 8.6% of patients diagnosed with single melanoma (P < 0.001). Germinal CDKN2A mutations were identified in 10.7% of patients with synchronous melanomas and genetic variants in MC1R in 72%. No significant differences in all survival outcomes between patients with synchronous melanomas and single melanomas were found.ConclusionSynchronous melanomas are more frequent than previously reported and are more frequent in older patients compared to single melanomas. Moreover, these patients have a higher risk of developing a new primary melanoma during follow‐up and have higher rates of germline susceptibility variants. Nevertheless, these findings were not associated with worse outcomes.
New contributions to the Cyrtophoria ciliates (Protista, Ciliophora): Establishment of new taxa and phylogenetic analyses using two ribosomal genes
Authors: Wang Congcong , Jiang Limin , Pan Hongbo , Warren Alan , Hu Xiaozhong 
Published: July 2022

AbstractPeriphytic ciliates play a vital role in the material cycle and energy flow of microbial food web, however, their taxonomy and biodiversity are inadequately studied given their high species richness. Two new and one little known species, viz. Derouxella lembodes gen. et sp. nov., Cyrtophoron multivacuolatum sp. nov., and Cyrtophoron apsheronica Aliev, 1991, collected from coastal waters of China, were investigated using modern methods. Derouxella gen. nov. can be recognized by having dorsoventrally flattened body, a podite, one fragmented preoral kinety, two parallel circumoral kineties, and somatic kineties progressively shortened from right to left. Morphological classification and phylogenetic analyses based on nuclear small subunit ribosomal RNA (nSSU rRNA) and mitochondrial small subunit ribosomal RNA (mtSSU rRNA) gene sequence data inferred that Derouxella gen. nov. occupies an intermediate position between Hartmannulidae and Dysteriidae. Cyrtophoron multivacuolatum sp. nov. is characterized by large body size, the numbers of somatic kineties and nematodesmal rods, and having numerous contractile vacuoles. The genus Cyrtophoron and the poorly known species C. apsheronica were redefined. Even with the addition of newly obtained nSSU rRNA and mtSSU rRNA gene sequences of Cyrtophoron, the family Chlamydodontidae was still recovered as a monophyletic group, the monophyly of Cyrtophoron was supported too.
Blue light‐induced phosphorylation of Arabidopsis cryptochrome 1 is essential for its photosensitivity
Authors: Gao Lin , Liu Qing , Zhong Ming , Zeng Nannan , Deng Weixian , Li Yaxing , Wang Dong , Liu Siyuan , Wang Qin 
Published: July 2022

AbstractPlants possess two cryptochrome photoreceptors, cryptochrome 1 (CRY1) and cryptochrome 2 (CRY2), that mediate overlapping and distinct physiological responses. Both CRY1 and CRY2 undergo blue light‐induced phosphorylation, but the molecular details of CRY1 phosphorylation remain unclear. Here we identify 19 in vivo phosphorylation sites in CRY1 using mass spectrometry and systematically analyze the physiological and photobiochemical activities of CRY1 variants with phosphosite substitutions. We demonstrate that nonphosphorylatable CRY1 variants have impaired phosphorylation, degradation, and physiological functions, whereas phosphomimetic variants mimic the physiological functions of phosphorylated CRY1 to constitutively inhibit hypocotyl elongation. We further demonstrate that phosphomimetic CRY1 variants exhibit enhanced interaction with the E3 ubiquitin ligase COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1). This finding is consistent with the hypothesis that phosphorylation of CRY1 is required for COP1‐dependent signaling and regulation of CRY1. We also determine that PHOTOREGULATORY PROTEIN KINASEs (PPKs) phosphorylate CRY1 in a blue light‐dependent manner and that this phosphorylation is critical for CRY1 signaling and regulation. These results indicate that, similar to CRY2, blue light‐dependent phosphorylation of CRY1 determines its photosensitivity.
Fluorescence fluctuation‐based super‐resolution microscopy: Basic concepts for an easy start
Authors: Alva Alma , Brito‐Alarcón Eduardo , Linares Alejandro , Torres‐García Esley , Hernández Haydee O. , Pinto‐Cámara Raúl , Martínez Damián , Hernández‐Herrera Paul , D'Antuono Rocco , Wood Christopher , Guerrero Adán 
Published: July 2022

AbstractDue to the wave nature of light, optical microscopy has a lower‐bound lateral resolution limit of approximately half of the wavelength of visible light, that is, within the range of 200 to 350 nm. Fluorescence fluctuation‐based super‐resolution microscopy (FF‐SRM) is a term used to encompass a collection of image analysis techniques that rely on the statistical processing of temporal variations of the fluorescence signal. FF‐SRM aims to reduce the uncertainty of the location of fluorophores within an image, often improving spatial resolution by several tens of nanometers. FF‐SRM is suitable for live‐cell imaging due to its compatibility with most fluorescent probes and relatively simple instrumental and experimental requirements, which are mostly camera‐based epifluorescence instruments. Each FF‐SRM approach has strengths and weaknesses, which depend directly on the underlying statistical principles through which enhanced spatial resolution is achieved. In this review, the basic concepts and principles behind a range of FF‐SRM methods published to date are described. Their operational parameters are explained and guidance for their selection is provided.
Mindfully aware and open: Mitigating subjective and objective financial vulnerability via mindfulness practices
Authors: Bayuk Julia , Lee Hyunjung Crystal , Park Jooyoung , Saka Serkan , Talukdar Debabrata , Sinha Jayati 
Published: July 2022

AbstractOur research presents mindfulness as a potential intervention to mitigate financial vulnerability, defined as the ability to handle unexpected future financial setbacks. As potential interventions to mitigate consumer financial vulnerability, we provide a conceptual framework on how two types of mindfulness practices (i.e., non‐judgmental awareness and openness to experience) can mitigate the subjective and objective financial vulnerability differently. We suggest ways to manipulate the two types of mindfulness and discuss the results of our initial pilot study, focusing on lower‐income consumers. In addition, we propose fruitful avenues for future research and provide recommendations for managers and policymakers to better address consumer financial vulnerability and enhance consumer welfare via mindfulness practice.
Ultrasonographic analysis of the calves for efficient botulinum toxin type A injection
Authors: Chong Yuming , Li Zhijin , Zhang Dingyue , Jin Lin , Chen Cheng , Yu Nanze , Long Xiao 
Published: July 2022

AbstractBackgroundLarge calves are considered unattractive. Botulinum toxin type A (BoNT‐A), acting as a neurotoxin that reduces muscle contraction, is widely used in calf contouring. However, there lacks detailed anatomical data to guide clinical injection.ObjectivesThis study aims to use ultrasonography to provide depth information of the calf muscles and determine the morphological types of the gastrocnemius muscle.MethodsUltrasound scanning was performed at six sites on 40 Chinese adults. A real‐time ultrasound scannerwas used to detect the depth of the gastrocnemius muscle and the soleus muscle.ResultsThe thickness of the gastrocnemius muscle and the soleus muscle was measured. The depth from the skin to the surface, the middle, and the bottom of the gastrocnemius muscle was measured. The morphology of gastrocnemius muscle was classified into four different types under ultrasound according to the muscle bulge pattern.ConclusionThis study took ultrasonic measurements of Asian calves to guide accurate and effective BoNT‐A injection in real clinical practice.
A quasi‐randomized controlled trial of Ninjin'yoeito for the treatment of postpartum anemia and the prevention of postpartum depression
Authors: Kawakami Sho‐ichi , Fukuda Shigeru , Shinbori Yoko , Umeki Michiko , Mitani Minoru , Kuramoto Akitaka , Sugimoto Chisato , Nitta Makoto , Higashi Kenji , Ito Masaharu 
Published: July 2022

AbstractAimTo study the effect of Ninjin'yoeito (NYT) on postpartum anemia and on the development of postpartum depression (PPD).MethodsIn this prospective, single‐center, open‐label, quasi‐randomized controlled trial, patients with anemia 1–2 days postdelivery were randomized to receive either NYT or an oral iron preparation for 4 weeks. The primary endpoint was the hemoglobin (Hb) level. Secondary endpoints were fatigue (assessed by the numerical rating scale [NRS]) and prevalence of postpartum depressive symptoms, as defined by an Edinburgh postnatal depression scale (EPDS) score ≥9. Hb levels and fatigue were measured before, and 4 weeks after, treatment and the EPDS was measured 4 weeks posttreatment.ResultsOf 1066 participants (NYT group: 532, iron group: 534) 1061 (NYT group: 529, iron group: 532) underwent full analysis. The Hb level increased significantly in both groups (p < 0.001), and there were no significant differences between the groups in terms of the change in Hb levels (NYT: 2.4 ± 0.8 g/dL vs. iron: 2.5 ± 0.7 g/dL, p = 0.098). Fatigue decreased significantly in the NYT group (p < 0.001) but did not change in the iron group, and the difference was significant (p < 0.001). There was a significant difference between the two groups in terms of the prevalence of postpartum depressive symptoms (NYT: 5.7% vs. iron: 9.4%, odds ratio [OR] = 0.58, 95% confidence interval [CI] = 0.36–0.93).ConclusionThe results suggest that NYT improves postpartum anemia and fatigue, and may be able to prevent the development of PPD.
Using a self‐guided app to provide communication strategies for caregivers of young children with developmental disorders: A pilot investigation
Authors: Romski Mary Ann , Sevcik Rose A. , King Marika , DeLeo Gianluca , Branum‐Martin Lee , Bornman Juan 
Published: July 2022

AbstractOne important evidence‐based component of early communication interventions in high‐income countries is teaching parents and other primary caregivers to provide communication opportunities in daily activities to stimulate the development of beginning communication skills. To address some of the barriers to communication interventions for children with developmental disorders (DD) in rural South Africa, we developed a prototype Web‐based self‐guided app for caregivers to use at home with their children with DD who were at the beginning stages of communication development. The purpose of this study is to examine how this app intervention functioned for caregivers and its secondary effects on their children. Fifty‐one caregiver‐child dyads were randomly assigned to either a typical care intervention group (a 30‐minute hospital‐based intervention once a month) or the self‐guided mobile health technology (MHT) app plus the typical care intervention. We assessed both the caregivers and their children. The majority of the 27 caregiver‐child dyads (81%) assigned to the app group used the app and completed a mean of 35.8 sessions across the 48 sessions (mean range = 5.08–15.75). Eighty percent of these caregivers employed the “help” function of the app (M per caregiver = 9.89). The caregivers who completed 44–48 sessions reported that more than half of the children moved from pre‐symbolic forms of communication (e.g., crying) to symbolic forms of communication (e.g., words) by the end of the intervention. Compared to the typical care group, the caregivers perceived that their children's success increased even though their difficulties remained stable. The app group showed a very modest gain in expressive language while the typical care group did not. The findings suggest that the self‐guided app framework shows promise as a supplement to traditional monthly speech‐language intervention in South Africa.
Hybridization and genome duplication for early evolutionary success in the Asian Palmate group of Araliaceae
Authors: Gallego‐Narbón Angélica , Wen Jun , Liu Jing , Valcárcel Virginia 
Published: July 2022

AbstractThe phenomenal advances in sequencing techniques and analytical development during the last decade have provided a unique opportunity to unravel the evolutionary history of lineages under complex patterns of evolution. This is the case of the largest clade of the ginseng family (Araliaceae), the Asian Palmate group (AsPG), where the large internal polytomies and genome incongruences detected in previous studies pointed to a scenario of radiation with hybridization events between genera for the early evolution of the group. In this study, we aim to obtain well‐resolved nuclear and plastid phylogenies of the AsPG using Hyb‐Seq to evaluate the radiation hypothesis and assess the role of hybridization in the early evolution of the group. We performed concatenated‐ and coalescent‐based phylogenetic analyses from the 936 targeted nuclear loci and 261 plastid loci obtained for 72 species representing 20 genera of the AsPG and the main clades of Araliaceae. The impact of hybridization and incomplete lineage sorting (ILS) was assessed with SNaQ, and genome duplications were evaluated with ChromEvol. Our nuclear and plastid phylogenies are compatible with a scenario of early radiation in the AsPG. Also, the identification of extensive signals of hybridization and ILS behind the genome incongruences supports hybridization as a major driving force during the early radiation. We hypothesize a whole‐genome duplication event at the origin of the AsPG, followed by a radiation that led to extensive ILS, which, alongside the early inter‐genera hybridization, is obscuring the phylogenetic signal in the early evolution of this major clade.
Captivity induces large and population‐dependent brain transcriptomic changes in wild‐caught cane toads (Rhinella marina)
Authors: Yagound Boris , West Andrea J. , Richardson Mark F. , Gruber Jodie , Reid Jack G. , Whiting Martin J. , Rollins Lee A. 
Published: July 2022

AbstractGene expression levels are key molecular phenotypes at the interplay between genotype and environment. Mounting evidence suggests that short‐term changes in environmental conditions, such as those encountered in captivity, can substantially affect gene expression levels. Yet, the exact magnitude of this effect, how general it is, and whether it results in parallel changes across populations are not well understood. Here, we take advantage of the well‐studied cane toad, Rhinella marina, to examine the effect of short‐term captivity on brain gene expression levels, and determine whether effects of captivity differ between long‐colonized and vanguard populations of the cane toad's Australian invasion range. We compared the transcriptomes of wild‐caught toads immediately assayed with those from toads captured from the same populations but maintained in captivity for seven months. We found large differences in gene expression levels between captive and wild‐caught toads from the same population, with an over‐representation of processes related to behaviour and the response to stress. Captivity had a much larger effect on both gene expression levels and gene expression variability in toads from vanguard populations compared to toads from long‐colonized areas, potentially indicating an increased plasticity in toads at the leading edge of the invasion. Overall, our findings indicate that short‐term captivity can induce large and population‐specific transcriptomic changes, which has significant implications for studies comparing phenotypic traits of wild‐caught organisms from different populations that have been held in captivity.
DNA methylation profiling improves routine diagnosis of paediatric central nervous system tumours: A prospective population‐based study
Authors: Schepke Elizabeth , Löfgren Maja , Pietsch Torsten , Olsson Bontell Thomas , Kling Teresia , Wenger Anna , Ferreyra Vega Sandra , Danielsson Anna , Dosa Sandor , Holm Stefan , Öberg Anders , Nyman Per , Eliasson‐Hofvander Marie , Sandström Per‐Erik , Pfister Stefan M. , Lannering Birgitta , Sabel Magnus , Carén Helena 
Published: July 2022

AbstractAimsPaediatric brain tumours are rare, and establishing a precise diagnosis can be challenging. Analysis of DNA methylation profiles has been shown to be a reliable method to classify central nervous system (CNS) tumours with high accuracy. We aimed to prospectively analyse CNS tumours diagnosed in Sweden, to assess the clinical impact of adding DNA methylation‐based classification to standard paediatric brain tumour diagnostics in an unselected cohort.MethodsAll CNS tumours diagnosed in children (0–18 years) during 2017–2020 were eligible for inclusion provided sufficient tumour material was available. Tumours were analysed using genome‐wide DNA methylation profiling and classified by the MNP brain tumour classifier. The initial histopathological diagnosis was compared with the DNA methylation‐based classification. For incongruent results, a blinded re‐evaluation was performed by an experienced neuropathologist.ResultsTwo hundred forty tumours with a histopathology‐based diagnosis were profiled. A high‐confidence methylation score of 0.84 or more was reached in 78% of the cases. In 69%, the histopathological diagnosis was confirmed, and for some of these also refined, 6% were incongruent, and the re‐evaluation favoured the methylation‐based classification. In the remaining 3% of cases, the methylation class was non‐contributory.The change in diagnosis would have had a direct impact on the clinical management in 5% of all patients.ConclusionsIntegrating DNA methylation‐based tumour classification into routine clinical analysis improves diagnostics and provides molecular information that is important for treatment decisions. The results from methylation profiling should be interpreted in the context of clinical and histopathological information.