bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein-to-protein interaction of genes responsible for the economic trait of Madura Cattle: an in silico analysis
Authors: Nugroho T , Prastowo S 
Published: December 2022

Abstract
Our previous study found five genes consist of Insulin-like growth factor-1 (IGF-1), Growth hormone (GH1), Growth hormone receptor (GHR), Myostatin (MSTN), and Leptin (LEP) are responsible for the economic traits of Madura Cattle. This paper aimed to identify the protein-to-protein interaction of genes responsible for the economic traits of Madura Cattle. In silico study was done using STRING v.11.5 with Bos taurus IGF1, GH1, GHR, MSTN, and LEP as input analysis. Results show 24 biological processes, 3 molecular functions, and 6 KEGG pathways as potential protein interactions between genes. All genes are involved in six biological processes i.e. response to the hormone, response to oxygen-containing compound, cell surface receptor signalling pathway, regulation of signal transduction, positive regulation of the cellular metabolic process, and positive regulation of the macromolecule metabolic process. Five genes were predicted as functional partner genes, namely Leptin receptor (LEPR), Tyrosine-protein kinase receptor (INSR), Activin receptor type-2b precursor (ACVR2B), Insulin-like growth factor 1 receptor (IGF1R), and Insulin-like growth factor-binding protein 3 (IGFBP3). Based on the results, we can demonstrate the protein-to-protein interaction of IGF-1, GH, GHR, MSTN, and LEP genes which are potentially related to each other. Further experimental study is required to validate the interaction.
Tenure of agricultural assets farmers affected by policy on national strategic project development in Klaten Regency
Authors: Utami B W , Hariadi S S , Raya A B 
Published: December 2022

Abstract
Klaten is one of the three regencies in Central Java affected by the construction of the Solo-Yogyakarta toll road, and at the same time, it is the widest area for constructing this route. The purpose of this article is to describe agricultural mastery by farmers as a national project strategy. This article is part of research on the Response of Affected Farmers to the Strategic National Project policy for toll development. A research method is a quantitative approach by taking as many as 251 respondents who are taken representatively from the east, west, central, north and south sides. The performance of the control of agricultural assets is seen from (1) the average area of land owned by farmers, (2) the percentage of farmers on the status of farmers’ land ownership, (3) the distribution of land ownership and control, (4) the distribution of farmers’ occupations, (5) income from agricultural sector and (6) sources of income in the agricultural sector. Data were analyzed descriptively analytically with frequency tabulation. In conclusion, most respondents have land ownership status as cultivators, owning 0.01 to 0.49 ha of land, and consider farming their main occupation.
Video art as environmental communication media for the problem of plastic waste in Solo
Authors: Riyanto Bedjo , Alimin Nurhayatu Nufut , Setyawan  
Published: December 2022

Abstract
Garbage is a big problem in the city of Solo. It can be seen in the Putri Cempo Landfill (TPA), which is the place where the final processing of waste from all areas of Surakarta City takes place. The waste management activities of the Putri Cempo harm the safety and comfort aspects of the environment as well as location and accessibility but also become an opportunity in the economic part. To campaign for Solo as a city that supports and cares about environmentally friendly issues, what art actors can do is disseminate the crisis that occurs through various media so that the public more easily understands it. One of the most effective ways to attract people today is through a video. They are supported by music that can foster empathy for the audience. Research methods, creation, and presentation are based on research and experimentation. The process of creating works consists of several stages, including the exploration stage, experimental experiment stage, improvisation stage, forming step, and finishing stage.
Fabric scrap interior accessories: A Solution to textile waste in Gulon Asri waste bank community in Solo, Indonesia
Authors: Alimin N N , Murni E S , Cahyani D , Aini N , Mulyono A 
Published: December 2022

Abstract
Indonesia is one of the world’s largest contributors to textile waste. Textile waste is difficult to decompose and takes an extremely long time to degrade safely without polluting the environment. This country has also turned into the world’s second-largest waste supplier. In the interior design sector, clothes are typically used as the primary medium to make textile-based products or upholstery. The interior can use fabric waste into a product with beauty, function, and economic value. This community service project report presents a process of turning textile waste into interior accessory products, such as blankets, carpets, and pillows. To this end, we utilized textile waste as the primary material for home accessories to enhance a house’s aesthetic value. This community service consisted of five stages, four of which have been completed and the final stage is still underway.
Yield and seed size stability analysis of black soybean lines derived from gamma rays irradiation
Authors: Iqbal M , Tarmizi  , Arwin  , Yuliasti  , Harsanti L , Anisiyah  , Puspitasari W 
Published: December 2022

Abstract
The development of black soybean superior varieties is still limited despite its importance in Indonesian cuisine. The gamma rays radiation can be utilized to develop new genetic materials derived from the existing cultivar. National black soybean variety Detam 1 was irradiated as the parental line to produce mutant lines for breeding material. This research was performed to identify the stable and superior genotypes from 13 candidate mutant lines tested in eight environments using stability parameters. Three genotypes showed significant superiority compared to wild-type parent, namely G5, G6, and G12 which showed an average yield of 2.99 t/ha, 3.21 t/ha, and 2.95 t/ha respectively. Based on the Finlay-Wilkinson stability test, 8 lines were considered stable yet only 4 can be further satisfied Eberhard-Russel method, namely G2, G5, G6, and G10. In comparison, stability analysis using AMMI suggested different stable lines: G2, G7, and G12. From this list, G12 and G6 also showed a beneficial large-sized seed trait significantly larger than the parent at 18.00 g and 18.96 g per 100 seeds, respectively.
Function and aesthetic contexts in disaster mitigation comics
Authors: Mataram S 
Published: December 2022

Abstract
The global climate change bears impact on natural conditions. In extreme contexts, these changes can trigger natural disasters. To avoid or minimize a more severe impact, disaster mitigation efforts are needed. A publication media that is able to persuade and attract attention is needed to convey these mitigation efforts to the public. Comics are a medium that can facilitate this. In addition to entertaining and attracting attention from the visual side, comics are also able to convey messages. Using comparative methods and content analysis in the form of qualitative descriptive study, this research aims to see how comic media attracts attention in terms of visual and aesthetic, and conveys the function of delivering messages to the public. Comics as a medium of conveying messages become more effectively accepted by the public if they are supported by attractive visual features. With its image as a medium of entertainment and light reading material, comics make people feel comfortable and not tense or afraid.
Effect of substrate nutrient content level on nutritional expression of Hermetia illucens: a meta-analysis
Authors: Alifian M D , Nahrowi  , Sumiati  , Sholikin M M 
Published: December 2022

Abstract
This research aims to examine the effect of substrate nutrient content on nutrient expression of Hermetia illucens by using a meta-analysis method The database was constructed from studies that have reported various substrates with nutrient levels in Hermetia illucens. The nutritional content observed in this study were dry matter (DM), ash, crude protein (CP), true protein (TP) and ether extract (EE). There were 12 articles that met the criteria entered in the database. Mixed model methodology was used for statistical analysis. The difference in study data on Hermetia illucens was used as a random effect, and nutrient substrate level was used as a fixed effect. The statistical model used is the p-value. If the significance of an effect occurs, the p-value <0.05. The results showed that the increase in ash, CP, and TP on the substrate had a significant effect on the increase in nutrient Hermetia illucens (P<0.05) and increasing DM substrate tends to increased DM on Hermetia illucens (P<0.1). Increased EE levels in the substrate did not affect EE levels in Hermetia illucens larvae (P>0.05). The conclusion of this study was that the increase in ash and CP levels on the substrate had an effect on increasing the nutrient content of ash, CP, and TP of Hermetia illucens larvae.
Estimated sediment exports and erosion in Central Citarum watershed
Authors: Suryanta J , Wahyudin Y , Darmawan M , Amhar F , Santikayasa I P , Nahib I 
Published: December 2022

Abstract
In addition to the problem of water availability and quality, the Citratum watershed is also experiencing problems with soil erosion and sedimentation. Climate change and land use cover are the leading causes of this. Therefore, monitoring water conditions and soil erosion is prioritized in the central Citarum watershed covering an area of 227,020 hectares. The purpose of this study was to determine soil erosion and the spatial distribution of sediment exports. The InVEST SDR method was used to calculate sediment exports and soil erosion. The results showed that the total sediment export of the Central Citarum watershed was 4.61 x million tons/year or an average of 20.31 tons/ha/year. Significant distributions occur in several sub-watersheds, including Cilawang, Cihalaya, Cipada, Citarum 58, and Cimurah, each contributing 36.56%, 14.83%, 13.70%, 4.73%, and 4.53 %. Meanwhile, total soil erosion is 23.16 million tons/year or an average of 102 tons/ha/year, with the most extensive distribution in the Cilawang sub-watershed, 35.12%. High sediment discharge occurs in areas with steep slopes, high rainfall, and dry land agriculture. Furthermore, efforts to reduce sediment exports to the Cirata and Jatiluhur reservoirs should focus on these five sub-watersheds.
Empowerment of Women Tea Pickers (A Case Study on Lawu Mountainside in Karanganyar Regency)
Authors: Wibowo A , Suwarto  , Winarno  , Anantanyu S , Permatasari P 
Published: December 2022

Abstract
The shift in women’s roles from domestic to public is a phenomenon in the development of the social, economic, and political reality of women. Women’s awareness of non-domestic roles is increasing but their involvement does not mean they are more empowered or that more attention is given to women’s rights. This research describes the shift in women’s roles and their contribution to meeting household needs, and how the empowerment of women is implemented. The research method is descriptive qualitative and the data analysis uses an interactive analysis. The research results show that there is a division of work in daily life, in which men work to fulfil their social needs while women have to bear the burden of their economic needs. This work division is seen in farm management, where men’s work is dominated by crops with a high economic value or livestock farming, while women’s work focuses on crops with a low economic values and small livestock. Empowerment of women is carried out in three ways: capacity building, cultural change, and structural adjustment, based on the social, political, and psychological strengths of women tea pickers.
Application of green budgeting in finance and development policy
Authors: Azzahra L , Pamungkas P , Trinarningsih W 
Published: December 2022

Abstract
Climate change and extreme weather have negative impacts on human life. Efforts are needed to prevent environmental damage. In the Indonesian economy, there is a Green Growth Program, which is a program to realize economic growth that can reduce poverty and ensure social inclusion, environmental sustainability, and resource efficiency. One of the programs is green budgeting which consists of actions and fiscal policy to prevent environmental problems. This study focuses on the relationship between green budgeting and financial and development policies, it aims to see how the proportion of the budget is set on finance and development. This study uses a quantitative approach by employing descriptive analysis. Green budgeting has been implemented in Indonesia, but it is facing various problems. At the local level, the government does not understand green budgeting. There is also a lack of data for formulating the next green budgeting policy. Lack of government commitment causes the budget for environmental management not as a priority. This causes the limited local government budget (APBD) allocated. Limited budget constraints have to be overcome by finding other sources, for example, prepare of sustainable programs or environmental approach.
The correlation between farmers motivation and perception with commitment to raising buffalo in Pemalang Regency
Authors: Muatip K , Purwaningsih H , Enstein A , Djatmiko O E , Mastuti S , Hidayat N N , Widiyanti R , Wakhidati Y N , Safitri L , Cahyo D N 
Published: December 2022

AbstractThis study aimed to determine the motivation and perception of buffalo farmers and their commitment to raising buffalo and to identify the correlation between motivation and perception with the commitment of buffalo farmers to maintain buffalo in the Pemalang Regency. The research used survey method. The determination of the area was carried out purposively in Pemalang Regency with the largest buffalo population in Central Java. Four sub-districts with large buffalo populations were chosen such as Taman, Pemalang, Bantarbolang, and Belik sub-districts. One of the village with the largest buffalo population was selected for each selected sub-district. Respondents were taken randomly and the number of respondents was determined using the Slovin formula with a margin of error of 10%, obtaining 212 respondents. The analytical method used was descriptive analysis and rank spearman. The results showed that the motivation of buffalo farmers with the medium to the high category, the perception of farmers was in the high category, and the commitment of buffalo farmers was in the medium to high category. The correlation between the motivation and perception with the commitment of farmers to raising buffalo was in a strong category (0.590 and 0.531).
Screening of simple sequence repeats (SSR) primers from mutated Indigofera zolligeriana Miq plants
Authors: Royani J I , Sinaga O F Br , Aliyah K N , Hardianto D , Agustina T , Rofiq M N , Kristamtini  , Handayani T , Puspitasari W , Sudarsono  , Abdullah L , Asiyah S I 
Published: December 2022

Abstract
The mutated plants from Indigofera zolligeriana were screened using 14 SSR primers, to know the polymorphic DNA profiles of mutated plants. For screening these primers we used 12 DNA genomes of mutated I. zolligeriana plants. DNA genomes from 12 mutated plants were isolated. Amplification of 12 DNA genomes using multiplex PCR method followed by optimization of the method to obtain the best band profiles. Screening with the 14 SSR primers showed that not of the 14 primers used could amplify DNA genomes of mutated I. zollingeriana. Five primers showed polymorphic bands at 59°C and 13 primers at 48°C. After all treatments, 12 primers are suitable for use in further DNA profiling of other mutated I. zolligeriana plants.
Utilization of tannin from chestnut as a protective agent in slow release urea: An in vitro rumen fermentation study
Authors: Feggie F , Nayohan S , Wiryawan K G , Jayanegara A 
Published: December 2022

Abstract
Urea has been utilized as a non-protein nitrogen supplement for ruminants due to its affordable price, abundantly available, easy to use, and positively affect microbial protein synthesis in the rumen. However, urea have a weakness since it is rapidly converted into ammonia in the rumen and may lead to nitrogen loss and even ammonia toxicity. Development of a slow release urea (SRU) is therefore important in order to overcome such potential negative impacts. This experiment was conducted to evaluate the effects of SRU, made by complexing the urea with tannin from chestnut, on in vitro rumen fermentation parameters. The treatments were the addition of different forms and levels of urea into a basal diet, i.e., 1% conventional urea (T0), 1% SRU (T1), 2% SRU (T2), and 3% SRU (T3). Data were analyzed by using analysis of variance and continued with a post-hoc test namely the Tukey’s test. Results revealed that the SRU treatments (T1-T3) reduced (P<0.05) ruminal ammonia concentration at various time point intervals than that of the conventional urea (T0). However, the SRU did not alter microbial protein synthesis and nutrient digestibility. In conclusion, SRU made from tannin is an effective supplement for enhancing nitrogen utilization in the rumen.
CD27 on human memory B cells–more than just a surface marker
Authors: Grimsholm Ola 
Published: December 2022

Summary
Immunological memory protects the human body from re-infection with an earlier recognized pathogen. This memory comprises the durable serum antibody titres provided by long-lived plasma cells and the memory T and B cells with help from other cells. Memory B cells are the main precursor cells for new plasma cells during a secondary infection. Their formation starts very early in life, and they continue to form and undergo refinements throughout our lifetime. While the heterogeneity of the human memory B-cell pool is still poorly understood, specific cellular surface markers define most of the cell subpopulations. CD27 is one of the most commonly used markers to define human memory B cells. In addition, there are molecular markers, such as somatic mutations in the immunoglobulin heavy and light chains and isotype switching to, for example, IgG. Although not every memory B cell undergoes somatic hypermutation or isotype switching, most of them express these molecular traits in adulthood. In this review, I will focus on the most recent knowledge regarding CD27+ human memory B cells in health and disease, and describe how Ig sequencing can be used as a tool to decipher the evolutionary pathways of these cells.
A randomized trial of a nurse-led educational intervention in patients with heart failure and their caregivers: impact on caregiver outcomes
Authors: Thodi Maria , Bistola Vasiliki , Lambrinou Ekaterini , Keramida Kalliopi , Nikolopoulos Petros , Parissis John , Farmakis Dimitrios , Filippatos Gerasimos 
Published: December 2022

Abstract

Aims
Psychoeducational interventions focusing on self-management of heart failure (HF) patients may improve patient health knowledge and reduce hospitalizations, but data regarding the effects on caregiver outcomes are inconclusive.

Methods and results
We conducted a single-centre, randomized controlled study to evaluate the effect of a nurse-led educational intervention in dyads of recently hospitalized HF patients and their caregivers on caregiver burden, feelings of guilt and health-related quality of life (HR-QoL). Dyads were randomized to usual care plus intervention group 1 (IG-1) or 2 (IG-2) or usual care only (control group, CG). Educational sessions in IG-1 and IG-2 were initiated before hospital discharge and continued with combination of home visits and telephone sessions in IG-1, or telephone sessions only in IG-2, delivered on regular intervals for 6 months. Caregiver burden was assessed by Heart Failure Caregiver Questionnaire (HF-CQ v5.0), guilt by Caregiver Guilt Questionnaire (CGQ), and QOL by EuroQol EQ-5D. Fifty-seven patient/caregiver dyads were included: 12 in IG-1, 18 in IG-2, and 27 in CG, of whom 11, 16, and 20, respectively, completed the study. All domains of HF-CQ and CGQ improved in IG-1 and IG-2 at 6 months, whereas deteriorated in CG (all P &lt; 0.01). EQ-5D improved in IG-1 and IG-2 only in visual analogue scale part (P = 0.002), but not in the descriptive part.

Conclusion
A nurse-led, 6-month educational intervention on recently hospitalized HF patients/caregiver dyads, delivered through either combined home visits and telephone sessions or telephone sessions only, reduced caregiver burden and feelings of guilt, with lesser effect on HR-QoL.

Registration
ClinicalTrials.gov: NCT05480969
Safety profile and long-term efficacy of very high-power short-duration (60–70 W) catheter ablation for atrial fibrillation: results of a large comparative analysis
Authors: Popa Miruna A , Bourier Felix , Lengauer Sarah , Krafft Hannah , Bahlke Fabian , Förschner Leonie V , Dorfmeister Stephan , Kathan Susanne , Telishevska Marta , Englert Florian , Lennerz Carsten , Reents Tilko , Hessling Gabriele , Deisenhofer Isabel , Kottmaier Marc 
Published: December 2022

AbstractAimsThis retrospective study sought to compare complication rates and efficacy of power-controlled very high-power short-duration (vHPSD) and conventional catheter ablation in a large cohort of patients with atrial fibrillation (AF).Methods and resultsWe analyzed 1115 consecutive patients with AF (38.7% paroxysmal, 61.3% persistent) who received first-time catheter ablation at our centre from 2015 to 2021. Circumferential pulmonary vein isolation ± additional substrate ablation using an irrigated-tip catheter was performed with vHPSD (70 W/5–7 s or 60 W/7–10 s) in 574 patients and with conventional power (30–35 W/15–30 s) in 541 patients. Baseline characteristics were well-balanced between groups (mean age 65.1 ± 11.2 years, 63.4% male). The 30-day incidence of cardiac tamponade [2/574 (0.35%) vs. 1/541 (0.18%), P = 0.598], pericardial effusion ≥ 10 mm [2/574 (0.35%) vs. 1/541 (0.18%), P = 0.598] and transient ischaemic attack [1/574 (0.17%) vs. 2/541 (0.37%), P = 0.529] was not significantly different between vHPSD and conventional ablation. No stroke, atrio-esophageal fistula, cardiac arrest or death occurred. Procedure (122.2 ± 46.8 min vs. 155.0 ± 50.5 min, P &lt; 0.001), radiofrequency (22.4 ± 19.3 min vs. 52.9 ± 22.0 min, P &lt; 0.001), and fluoroscopy (8.1 ± 7.2 vs. 9.2 ± 7.4, P = 0.016) duration were significantly shorter in the vHPSD group. At 12 months follow-up, freedom of any atrial arrhythmia was 44.1% vs. 34.2% (P = 0.010) in persistent AF and 78.1% vs. 70.2% in paroxysmal AF (P = 0.068).ConclusionvHPSD ablation is as safe as conventional ablation and is associated with an improved long-term efficacy in persistent AF.