bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hydroxyl radical enhanced carbon dots fluorescence quenching immunoassays for simultaneous detection of six kinds of antibiotics
Authors: Li Shijie , Nie Linqing , Wen Wenjun , Wang Zicheng , Wang Junping , Wang Shuo 
Published: November 2022

AbstractHydroxyl radical (•OH)‐sensitive carbon dots (CDs) with an excitation wavelength of 390 nm and emission wavelength of 525 nm were synthesized by microwave in one‐step. Using CDs as fluorescent probes, an •OH‐enhanced fluorescence quenching detection signal based on horseradish peroxidasecatalyzed H2O2 was introduced on the basis of enzyme‐linked immunoassay, and a •OH‐enhanced label‐free fluorescence quenching immunoassay (FQIA) was constructed for high‐sensitivity detection of six broad‐spectrum antibiotics. When used for nitrofuran Q2 metabolites (3‐amino‐1,3‐oxazolidin‐2‐one, 3‐amino‐5‐(morpholin‐4‐ylmethyl)‐1,3‐oxazolidin‐2‐one, 1‐aminohydantoin hydrochloride, semicarbazide hydrochloride), chloramphenicol (CAP) and florfenicol (FLR) detection, FQIAs achieved high sensitivity detection of 0.061, 0.0058, 0.064, 0.045, 0.015, and 0.01 ng/ml, respectively. Compared with ELISA, the detection sensitivity was improved by 2.03‐ to 7.8‐fold. On this basis, the sample pretreatment methods for six targets were optimized, and the simultaneous extraction and high‐sensitivity detection of six targets were achieved. The FQIA proposed in this work improved the detection sensitivity, reduced the sample consumption and pretreatment steps, shortened the extraction time, and improved the detection efficiency, which provided support for the development and application of new immunoassay products and the development of the rapid analysis industry.
Physicochemical parameters and nutritional profile of back and abdomen muscle of fresh golden pompano (Trachinotus ovatus) and hybrid grouper (Epinephelus lanceolatus × Epinephelus fuscoguttatus)
Authors: Ali Ahtisham , Wang Jinfang , Khan Imran , Wei Shuai , Sun Qinxiu , Xia Qiuyu , Wang Zefu , Han Zongyuan , Liu Shucheng 
Published: November 2022

AbstractGolden pompano (Trachinotus ovatus) and hybrid grouper (Epinephelus lanceolatus × Epinephelus fuscoguttatus) has widely been distributed in China and Southeast Asian countries with great commercial importance. In this study, the nutritional profiles, chemical and physical parameters of back and abdomen muscles were determined. Significantly different (p < .05) proximate compositions were found in two fish muscles. The contents of water‐soluble protein, salt‐soluble protein, and non‐nitrogenous protein were higher in the golden pompano while salt‐insoluble proteins were higher in the hybrid grouper. The main minerals found were K (3700.56–4495.57 μg/g) followed by P > Na > Mg > and Ca, respectively. Fatty acids contents consisted of polyunsaturated fatty acids ranging from 29.40% to 43.09% and saturated fatty acids 28.33% to 39.61%. The muscles were rich in n‐3 PUFAs with n‐6/n‐3 ratio of 1.36%–2.96% in the back and abdomen. On the other hand, total amino acid and non‐essential amino acid contents were found higher in the hybrid grouper while essential amino acid and delicious amino acid contents were higher in the golden pompano. Glutamic acid was the most predominant amino acid. The amino acid scores (AAS) of six amino acids were close to 1.00, whereas lysine showed the highest AAS while tryptophan was the most limited essential amino acid in all muscles, respectively. These results indicated golden pompano and hybrid grouper exhibited a varied nutritional composition and offered a good nutritional profile.
Application of inulin in bread: A review of technological properties and factors affecting its stability
Authors: Mohammadi Faezeh , Shiri Ameneh , Tahmouzi Sima , Mollakhalili‐Meybodi Neda , Nematollahi Amene 
Published: November 2022

AbstractDue to its dual function, inulin is an important prebiotic compound in the cereal industry, especially in bread production. In other words, improving technological features and creating health properties (such as reducing the risk of type 2 diabetes, heart disease, metabolic syndrome, and osteoporosis) have led to the widespread use of this compound. Inulin has many important technological functions in bread, including its ability to interact with water, create structure, and influence rheological properties, texture, and overall acceptability of the final product. Nevertheless, bread processing conditions can influence the structural integrity of inulin and thus affect its technological efficiency. Therefore, this review article aims to investigate the technological properties and factors affecting the stability of inulin during bread processing conditions. Generally, the addition of inulin could considerably improve the technical performance of bread. However, the stability of inulin depends on the formulation components, type of fermentation, and baking process.
Environmental enrichment and social isolation modulate inhibitory transmission and plasticity in hippocampal area CA2
Authors: Loisy Maïthé , Farah Amel , Fafouri Assia , Fanton Aurélien , Ahmadi Mahboubeh , Therreau Ludivine , Chevaleyre Vivien , Piskorowski Rebecca A. 
Published: November 2022

AbstractEnvironmental factors are well‐accepted to play a complex and interdependent role with genetic factors in learning and memory. The goal of this study was to examine how environmental conditions altered synaptic plasticity in hippocampal area CA2. To do this, we housed adult mice for 3 weeks in an enriched environment (EE) consisting of a larger cage with running wheel, and regularly changed toys, tunnels and treats. We then performed whole‐cell or extracellular field recordings in hippocampal area CA2 and compared the synaptic plasticity from EE‐housed mice with slices from littermate controls housed in standard environment (SE). We found that the inhibitory transmission recruited by CA3 input stimulation in CA2 was significantly less plastic in EE conditions as compared to SE following an electrical tetanus. We demonstrate that delta‐opioid receptor (DOR) mediated plasticity is reduced in EE conditions by direct application of DOR agonist. We show that in EE conditions the overall levels of GABA transmission is reduced in CA2 cells by analyzing inhibition of ErbB4 receptor, spontaneous inhibitory currents and paired‐pulse ratio. Furthermore, we report that the effect of EE of synaptic plasticity can be rapidly reversed by social isolation. These results demonstrate how the neurons in hippocampal area CA2 are sensitive to environment and may lead to promising therapeutic targets.
Normative Values of Neuromelanin‐Sensitive MRI Signal in Older Adults Obtained Using a Turbo Spin Echo Sequence
Authors: Al Haddad Rami , Chamoun Mira , Tardif Christine L. , Guimond Synthia , Horga Guillermo , Rosa‐Neto Pedro , Cassidy Clifford M. 
Published: November 2022

BackgroundThe integrity and function of catecholamine neurotransmitter systems can be assessed using neuromelanin‐sensitive MRI (NM‐MRI). The relevance of this method to neurodegenerative and psychiatric disorders is becoming increasingly evident, and it has potential as a clinical biomarker.PurposeTo support future application of NM‐MRI as a clinical biomarker by defining the normative range of NM‐MRI signal and volume metrics in cognitively normal older adults.Study TypeProspective.PopulationA total of 152 cognitively normal older adults aged 53–86 years old, including 41 participants who had follow‐up NM‐MRI data collected 9–16 months later.Field Strength/SequenceA 3.0 T; NM‐MRI turbo spin echo and T1‐weighted magnetization‐prepared rapid acquisition with gradient echo sequences.AssessmentNM‐MRI images were processed to yield summary measures of volume and signal (contrast‐to‐noise ratio, CNR) for the substantia nigra (SN) and locus coeruleus (LC) using a recently developed software employing a fully automated algorithm. Change in these metrics over time was also assessed.Statistical TestsMean and standard deviation of NM‐MRI metrics were calculated; change over time was tested for significance using 1‐sample t‐tests. P values < 0.05 were considered statistically significant.ResultsAt baseline SN signal (CNR) was 10.02% (left) and 10.28% (right) and LC signal was 24.71% (left) and 20.42% (right). Baseline SN volume was 576 mm3 (left) and 540 mm3 (right) and LC volume was 6.31 mm3 (left) and 6.30 mm3 (right). The only NM‐MRI metric showing significant change was a decrease in left SN volume (t40 = −2.57, P = 0.014).Data ConclusionWe report normative values for NM‐MRI signal and volume in the SN and LC of cognitively normal older adults and explore their change over time. These values may help future efforts to use NM‐MRI as a clinical biomarker by facilitating identification of patients with extreme NM‐MRI values.Level of Evidence2.Technical Efficacy Stage1.
Using reported pathogenic variants to identify therapeutic opportunities for genetic diseases
Authors: Ressler Andrew K. , Goldstein David B. 
Published: November 2022

AbstractPurposeDrug development strategies for genetic diseases depend critically on accurate knowledge of how pathogenic variants cause disease. For some well‐studied genes, the direct effects of pathogenic variants are well documented as loss‐of‐function, gain‐of‐function or hypermorphic, or a combination of the two. For many genes, however, even the direction of effect of variants remains unclear. Classification of Mendelian disease genes in terms of whether pathogenic variants are loss‐ or gain‐of‐function would directly inform drug development strategies.MethodsWe leveraged the recent dramatic increase in reported pathogenic variants to provide a novel approach to inferring the direction of effect of pathogenic variants. Specifically, we quantify the ratio of reported pathogenic variants that are missense compared to loss‐of‐function.ResultsWe first show that for many genes that cause dominant Mendelian disease, the ratio of reported pathogenic missense variants is diagnostic of whether the gene causes disease through loss‐ or gain‐of‐function, or a combination. Second, we identify a set of genes that appear to cause disease largely or entirely through gain‐of‐function or hypermorphic pathogenic variants.ConclusionsWe suggest a set of 16 genes suitable for drug developmental efforts utilizing direct inhibition.
A reliable quantitative method for determining CBD content and release from transdermal patches in Franz cells
Authors: Yu Liyun , Madsen Frederikke Bahrt , Eriksen Sofie Helvig , Andersen Aaron J. C. , Skov Anne Ladegaard 
Published: November 2022

AbstractIntroductionThere are several cannabidiol (CBD) transdermal patches available on the market. However, none are FDA‐approved. Furthermore, not much evidence has been published about CBD release and skin permeation from such patches, so the effectiveness and reliability remain unclear.ObjectivesWe aimed to develop a method to determine the in vitro release and skin permeation of CBD from transdermal patches using Franz cell diffusion in combination with quantitative 1H‐NMR (qNMR).Materials and MethodsThe study was conducted on CBD patches with known CBD content and six different commercially available or market‐ready CBD patches using a Franz cell with a Strat‐M™ membrane and with samples taken directly from the transdermal patch for qNMR analysis.ResultsThe use of qNMR yielded an average recovery of 100% ± 7% when samples with known CBD content were tested. Results from the testing of six commercially available patches indicated that five out of six patches did not contain the CBD amount stated by the manufacturer according to a ± 10% variance margin, of which four patches were under‐labeled and one was over‐labeled. The release rate of patches was determined, and significant differences between the patches were shown. Maximum release of CBD was calculated to occur after 39 to 70 h.ConclusionThe established method was proven to be a reliable means of determining the quantity and release of CBD from transdermal patches and can be used to verify CBD content and release rate in transdermal patches.
Evaluation of fatigue performance of filled and unfilled natural rubber/styrene‐butadiene rubber composites
Authors: Bakhshizade Ali , Ghasemi‐Ghalebahman Ahmad , Hajimousa Mohammad Ali 
Published: November 2022

AbstractThis paper deals with the fatigue life analysis of a blend of natural rubber (NR) and styrene‐butadiene rubber (SBR) with and without nanoclay particles. Various damage parameters based on strain are investigated. A nonlinear finite element analysis is carried out by using ABAQUS. To formulate the life prediction models, the measured fatigue life is used together with various damage parameters. It is shown that all the damage parameters can estimate the fatigue lives effectively with correlation coefficients greater than 0.9. There is a good agreement between the obtained fatigue live predictions and the measured fatigue results. The effect of various parameters such as true strain and nanoparticles' loading is also investigated. The results of sensitivity analysis show that the strain has a greater effect on the variation of the rubber compounds' fatigue life. The test samples' fracture surface is assessed via scanning electron microscopy (SEM). SEM results show that as the strain increases, the test samples softly fail while the fracture surface of the nanocomposite is roughened by the addition of nanoclay.
Performance of different body weights in the Cockcroft‐Gault equation in critically ill patients with and without augmented renal clearance: A multicenter cohort
Authors: Cucci Michaelia D. , Gerlach Anthony T. , Mangira Caroline , Murphy Claire V. , Roberts Jason A. , Udy Andrew A. , Dowling Thomas C. , Mullen Chanda L. 
Published: November 2022

AbstractStudy objectiveThe primary objective was to evaluate the performance of the Cockcroft‐Gault (CG) equation with different body weights (BWs) compared to a measured creatinine clearance (mCrCl) in an intensive care unit (ICU) population with and without augmented renal clearance (ARC).DesignMulticenter, retrospective cohort.SettingTwo ICUs in the United States and four ICUs from a previous international observational analysis.PatientsAdult ICU patients admitted from January 1, 2010 to July 30, 2020 with at least one mCrCl collected within the initial 10 days of hospitalization were eligible for inclusion.Measurements and main resultsThe primary outcome was the performance of the CG equation in ARC (mCrCl≥130 ml/min/1.73 m2) and non‐ARC (mCrCl<130 ml/min/1.73 m2) patients. Correlation was analyzed by Pearson's correlation coefficient, bias by mean difference, and accuracy by the percentage of patients within 30% of the mCrCl. A total of 383 patients were included, which provided 1708 mCrCl values. The majority were male (n = 239, 62%), median age of 55 years [IQR 40–65] with a surgical diagnosis (n = 239, 77%). ARC was identified in 229 (60%) patients. The ARC group had lower Scr values (0.6 [0.5–0.7] vs. 0.7 [0.6–0.9] mg/dl, p < 0.001) and higher mCrCl (172.8 (SD 39.1) vs. 89.9 mL/min/1.73 m2 (SD 25.4), p < 0.001) compared with the non‐ARC group, respectively. Among non‐ARC patients there was a moderate correlation (r = 0.33–0.39), moderate accuracy (range 48–58%), and low bias (range of −12.9 to 17.1) among the different BW estimations with the adjusted BW having the better performance. Among ARC patients there was low correlation (r = 0.24–0.28), low to moderate accuracy (range 38–70%), and high bias (range of −58.5 to −21.6).ConclusionsThe CG‐adjusted BW had the best performance in the non‐ARC patients, while CG performed poorly with any BW in ARC patients. Although the CG equation remains the standard equation for estimating CrCl in the ICU setting, a new, validated equation is needed for patients with ARC.
A High‐Efficiency MRI Contrast Agent as well as a Tumor Therapeutic Agent
Authors: Zhang Nan , Meng Lin , Wang Lu , Zhang Butian , Sun Hongchen , Zhang Kai 
Published: November 2022

AbstractInorganic/polymercore/shell nanoparticles (NPs) for theranostics have always attracted research interest due to their flexible composition and facile synthesis. Here, core/shell NPs are prepared with gadolinium hydroxide nanorods cores and Mn ion‐doped polydopamine shells. After PEG modification, the relaxation rate of Gd(OH)3@MnPDA‐PEG NRs achieved 35 s−1mm−1. The characterization results indicate that the predoping Mn ions obviously promote the effect of MRI compared with the nanorods without or with postdoping Mn ions, and the predoping method can effectively control the actual doping account of metal ions. Considering the toxicity, dopamine and manganese in molar ratio of 8:1 is finally chosen. Gd(OH)3@MnPDA‐PEG nanorods are conducted in vivo and in vitro imaging and therapeutic experiments. The results show that the nanorods has low biological toxicity, excellent magnetic resonance imaging effects, clear photoacoustic imaging, and photothermal therapy effects.
Reinforcement learning‐based robust optimal output regulation for constrained nonlinear systems with static and dynamic uncertainties
Authors: Jin Peng , Ma Qian , Zhou Guopeng , Miao Guoying 
Published: November 2022

AbstractThis article investigates the robust optimal output regulation problem for the constrained uncertain nonlinear systems. A two‐step design framework is proposed to overcome the difficulties brought by uncertainties, constraint and performance optimization. First, the feedforward controller is designed by the internal model principle, and the robust output regulation problem is transformed into the robust stabilization problem. Then, the optimal control problem with input saturation is further considered in the robust feedback controller design process. With the help of a non‐quadratic cost functional, actor‐critic algorithm and robust redesign technique are brought together to design the constrained robust optimal feedback controller. Finally, stability analysis based on Lyapunov method shows that all the signals of the closed‐loop system remain bounded, and the tracking error is uniformly ultimately bounded with arbitrarily small ultimate bound. Simulation results illustrate the effectiveness of the proposed methodology.
Sunlight‐induced Photocatalytic Degradation of Methylene Blue Dye by Magnesium and Silver‐based Cadmium Sulfide Nanoparticles
Authors: Jasmine Jeyakumar , Ponvel Kanagasabai Muruganandam 
Published: November 2022

AbstractThe photodegradation activity of nanomaterials is generally studied and used for water purification purposes. The present investigation reports the synthesis of magnesium and silver‐based cadmium sulfide nanoparticles (Mg2Ag2CdS4 NPs) with a size of ∼25 nm for the degradation of methylene blue (MB) dye. The optical properties of the nanoparticles were studied, and the bandgap energy of the nanoparticles was found to be 2.16 eV. The photocatalytic degradation showed the highest photocatalytic activity (88 %) for 90 min. The effects of operating parameters such as the amount of catalyst, initial pH, and the initial dye concentration were studied. The rate constants for various doses were calculated and followed the pseudo‐first order. A 75 % degradation of MB dye was found even after five uses. The photocatalyst can significantly contribute to the removal of hazardous toxic chemicals from aqueous solutions from an environmental point of view.
Electrochemical Activation Applied to Perovskite Titanate Fibers to Yield Supported Alloy Nanoparticles for Electrocatalytic Application
Authors: Xu Min , Liu Chencheng , Naden Aaron B. , Früchtl Herbert , Bühl Michael , Irvine John T. S. 
Published: November 2022

AbstractActive bi‐metallic nanoparticles are of key importance in catalysis and renewable energy. Here, the in situ formation of bi‐metallic nanoparticles is investigated by exsolution on 200 nm diameter perovskite fibers. The B‐site co‐doped perovskite fibers display a high degree of exsolution, decorated with NiCo or Ni3Fe bi‐metallic nanoparticles with average diameter about 29 and 35 nm, respectively. The perovskite fibers are utilized as cathode materials in pure CO2 electrolysis cells due to their redox stability in the CO/CO2 atmosphere. After in situ electrochemical switching, the nanoparticles exsolved from the perovskite fiber demonstrate an enhanced performance in pure CO2 electrolysis. At 900 °C, the current density of solid oxide electrolysis cell (SOEC) with 200 µm YSZ electrolyte supported NiFe doped perovskite fiber anode reaches 0.75 Acm−2 at 1.6 V superior to the NiCo doped perovskite fiber anode (about 1.5 times) in pure CO2. According to DFT calculations (PBE‐D3 level) the superior CO2 conversion on NiFe compared to NiCo bi‐metallic species is related to an enhanced driving force for C‐O cleavage under formation of CO chemisorbed on the nanoparticle and a reduced binding energy of CO required to release this product.
Vertically Oriented Porous PET as Template to Integrated Metal Halide for High‐Performance Large‐Area and Ultra‐Flexible X‐Ray Detector
Authors: Wang Shuo , Lei Yutian , Chen Huanyu , Peng Guoqiang , Wang Qian , Wang Haoxu , Duan Jinglai , Jin Zhiwen 
Published: November 2022

AbstractHigh‐performance X‐ray detectors have immense potential in medical and security inspections. However, the current X‐ray detectors are limited in flexible, high‐spatial‐resolution large‐scale detection, and integration for imaging. Here, nuclear track‐etched porous polyethylene terephthalate (PET) is developed as the template for preparing uniform, large‐area (≥105 cm2), and flexible metal halide (MH)‐based X‐ray detectors. Adjustable high‐density vertically oriented porous PET with adjustable thickness can provide proper physical support for flexible thick absorption film, thus improving X‐ray absorption ability with excellent bending stability. Moreover, vertical channels can block the ion migration, lateral charge diffusion, and water/oxygen attacks, increasing activation energy for ionic transport, charge collection rate of electrodes, and environmental stability. Hence, the related detectors eventually obtain large sensitivity (6722 µC Gyair−1 cm−2), low detection limit (1.87 nGyair s−1), and high spatial resolution (5.17 lp mm−1) compared to the detectors without porous PET template. Meanwhile, the device shows no degradation after storage or working under various thermal attacks. MH‐filled‐PET is also monolithically integrated on the bottom circuit with different MHs and it is applied to single‐pixel mode and fast linear‐array imaging in a broad range of X‐rays photon energy (20 to 160 keV).