bluuebunny/crossref_metadata_2025_split huggingface dataset:
The significance of cellular senescence hub genes in the diagnosis and subtype classification of a comprehensive database of gene expression in intervertebral disc degeneration
Authors: Liu Fei , Yin Ji , Wang Zhaoqiang , Cheng Kang , Song Chao , Cai Weiye , Guo Daru , Gao Silong , Jiang Yan , Liu Zongchao 
Published: November 2022

Abstract
Intervertebral disc degeneration (IVDD) is a complex age-related physiological change involving mainly cellular senescence (CS), inflammation, impaired tissue biomechanical function, and degradation of the extracellular matrix, and is a major cause of lumbar disc herniation and low back pain. Nonetheless, the specific role of CS and its associated genes in IVDD remains unclear. In this study, we first obtained 53 differentially expressed CS-related genes (CSRGs) by differential analyses of IVDD patients and non-IVDD patients from the GSE124272 and GSE150408 microarray expression datasets of the GEO database. We then performed GO and KEGG pathway enrichment analysis on these 53 CSRGs to explore their functions and pathways. To find the key genes from these 53 CSRGs, we first built a protein-protein interaction (PPI) network to recognize hub genes, and then on top of that we also applied the support vector machine recursive feature elimination (SVM-RFE) algorithm, random forest (RF) algorithm, and least absolute shrinkage and selection operator (LASSO) analysis. We finally obtained 4 hub CSRGs (DUSP3, MAPKAPK5, SP1 and VEGFA) to forecast the risk of IVDD. Based on the four hub genes we previously obtained, we built a nomogram model and performed a decision curve analysis, which ultimately suggested that the model was beneficial to patients. Based on the selected 4 hub CSRGs, we classified IVDD patients into two Hub gene patterns (hub gene clusters A and B) by the consensus clustering method, while the 297 DEGs obtained by screening based on the two hub gene clusters were classified into two gene patterns using the same method. We then applied a PCA algorithm to determine Hub gene scores for each sample to measure Hub gene patterns and found that patients in cluster A had higher Hub gene scores than those in cluster B. We also showed the correlation of two Hub gene patterns and Gene patterns with immune cell infiltration and the differential expression levels of four Hub genes by constructing heat maps and histograms. We performed GO enrichment analysis on these 297 DEGs to explore their role in IVDD. Finally, we used qPCR analysis and western blot to verify the expression levels of mRNA and protein in normal and IVDD cells of 4 hub CSRGs. In summary, CSRGs play an important role in the pathogenesis of IVDD, and our study of the hub gene cluster may guide future therapeutic strategies for IVDD.
Under Recognition and Treatment of Lymphedema in Head and Neck Cancer Survivors – A Database Study
Authors: Stubblefield Michael D. , Weycker Derek 
Published: November 2022

Abstract
Purpose: Head and neck cancer (HNC) will be diagnosed in approximately 54,000 Americans in 2022 with more than 11,000 dying as a result. The treatment of HNC often involves aggressive multimodal therapy including surgery, radiotherapy, and systemic therapy. HNC and its treatments are associated with multiple painful and function-limiting neuromusculoskeletal and visceral long-term and late effects. Among these is head and neck lymphedema (HNL), the abnormal accumulation of protein rich fluid, in as many as 90% of survivors. Though HNL is common and potentially contributory to other function-limiting issues in this population, it is notoriously understudied, underrecognized, underdiagnosed, and undertreated. This study seeks to determine the incidence of HNC-related lymphedema diagnosis and treatment in a large US healthcare claims repository database.
Methods: A retrospective observational cohort design and data from an integrated US healthcare claims repository—the IBM MarketScan Commercial Claims and Encounters (CCAE) and Medicare Supplemental and Coordination of Benefits (MDCR) Databases spanning the period April 1, 2012 through March 31, 2020.
Results: Of the 16,654 HNC patients eligible for evaluation, 1,082 (6.5%) with a diagnosis of lymphedema were identified based on eligibility criteria. Of the 521 HNC patients evaluated for lymphedema treatment, 417 (80.0%) patients received 1.5 courses of MLD, 71 (13.6%) patients were prescribed compression garments, and 45 (8.6%) patients received an advanced pneumatic compression device.
Conclusion: HNL in this population of HNC survivors was underdiagnosed and treated compared with contemporary assessments HNL incidence.
Bridging the gap: Can a patient questionnaire together with staff education increase diagnostics of endometriosis in primary care? -A pilot study
Authors: Westin Maria Öberg , Wikberg Carl , Björkelund Cecilia , Ariai Nashmil , Lindahl Ann-Christine , Hange Doninique 
Published: November 2022

Abstract
Objectives: To evaluate if a brief educational intervention for primary health care staff regarding endometriosis gives sustainable knowledge of endometriosis symptoms and if a brief questionnaire for women seeking care for common symptoms is feasible in the Swedish primary care context.
Design: Pilot study
Setting: 3 primary care centres (PCCs) in Sweden. Exploring knowledge among staff about endometriosis at baseline and 3 months after an information workshop. Evaluation of feasibility of a brief questionnaire for women seeking care for common symptoms For PCC personnel: knowledge about endometriosis at baseline and after 3 months. For patient questionnaire: whether adequate, understandable, acceptable, and feasible.
Participants: Females in primary care centre waiting room, and staff members at participating PCCs.
Results: The knowledge level of endometriosis was improved or sustained after 3 months compared to baseline among staff at PCCs. Over 90% of the patients stated that the questionnaire was adequate, understandable, acceptable, and feasible.
Conclusion: We found that an educational programme improved the staff’s knowledge about endometriosis. The programme together with the patient questionnaire could be a way to enhance knowledge about endometriosis among PCCs. This combined effort might facilitate earlier detection and treatment of women with endometriosis.
Association of nocturia with estimated glomerular filtration rate: a cross-sectional study from the NHANES 2005-2018
Authors: Song Jianling , Ke Ben , Fang Xiangdong 
Published: November 2022

Abstract
Background
Nocturia is a manifestation of systemic disease, of which chronic kidney disease is an independent predictor of nocturia because of its osmotic diuretic mechanism. However, to our knowledge, previous studies have not examined the relationship between nocturia and eGFR.
Objective
The purpose of this study was to assess the association between nocturia exposure and eGFR in the general US population.
Methods
Our study was a cross-sectional examination of 12,265 regular Americans who took part in the rvey NHANES between 2005 and 2018. After controlling for gender, age, race, fasting blood glucose, glycosylated hemoglobin, ALT, AST, TG, CHOL, HDL, LDL, BMI, diabetes, hypertension, alcohol consumption, tobacco use and PHQ9 score, a multiple regression analysis was performed to determine the association between nocturia and eGFR. Covariate-specific hierarchical analysis and interaction testing were performed. A sensitivity analysis was then conducted between the CKD group, the non-CKD group, and the various CKD prognostic risk groups.
Results
Multiple regression analysis showed that the frequency of nocturia increased by 1 time, and eGFR decreased 2.0 mL/min/1.73m2 (95%CI: -2.4, -1.7). Compared with no nocturia, the decrease of eGFR was the most significant 4 times per night (95%CI: -10.7, -5.4). Our results remained stable in the CKD and non-CKD groups. Within all CKD prognostic risk groups, only the moderately increased risk and low-risk groups had comparable results.
Conclusions
Increased frequency of nocturia was associated with a decrease in eGFR, regardless of the diagnosis of CKD. However, no correlation between nocturia and eGFR was observed in very high risk and high risk groups.
The Next Generation Fornax Survey (NGFS): Satellite Environmental Regions.
Authors: Blaña Matías , Puzia Thomas , Ordenes-Briceño Yasna , Tissera Patricia , Mora Marcelo , Ziliotto Tuila , Miller Bryan , Johnston Evelyn , Eigenthaler Paul , Galaz Gaspar , Pallero Diego 
Published: November 2022

Abstract
All satellite galaxies experience environmental secular evolution as they are accreted onto their host systems. Observations and simulations indicate that the closer they are to their hosts, the higher their dark matter densities are. Using the NGFS deep photometric observations of 630 satellites in the Fornax galaxy cluster we discover that their mean stellar densities also show such anti-correlation with their distances to the cluster center, correlating with the host tidal density field, up to a transition radius at ℜ★ ≈ 0.4𝑹vir (400 kpc), beyond which the stellar densities increase again. Comparisons with satellites of a Fornax-type cluster in the TNG50 simulation reveal a similar break at ℜ★ ≈ 0.5𝑹vir in deprojection, while their mean dark matter densities keep decreasing with distance to their host. Moreover, the relative fraction of gas-rich satellites and gaseous densities decrease significantly within ℜ★. We expand our analysis to satellites in different environments probing the more massive Virgo cluster, and also the less massive Milky Way and Andromeda systems, finding a similar behavior and transition radius in units of 𝑹vir for each system of satellites, with ℜ★ ∼ 0.4 − 0.6𝑹vir. This transition radius, if universal, could provide a useful observational diagnostic tool to identify the regions where the cluster environment would have processed the baryonic component of the accreted satellites more strongly, and where the more pristine population would be located. Finally, we suggest the utility of calibrating ℜ★ as a possible photometric virial mass estimator.
Correlates of Posttraumatic Stress in Cancer Patients and Their Family Caregivers Across Two Years Following Diagnosis
Authors: Mitchell Hannah-Rose , Antoni Michael H. , Shaffer Kelly M. , Kim Youngmee 
Published: November 2022

Abstract
Purpose: A major illness is traumatic for patients and families, posing risk for disease-related posttraumatic stress symptoms (PTSS). There is little available information to identify the cancer patients and caregivers at greatest risk for PTSS. This study examines the risk factors of PTSS in colorectal cancer.
Method: Patients with colorectal cancer and their caregivers (N=130 dyads) self-reported sociodemographic, psychosocial, and medical factors at diagnosis/treatment initiation (T1), at 1- (T2), and 2-years (T3) post-diagnosis. Cancer-related PTSS of intrusion, avoidance, altered arousal and reactivity (IES-R), and negative alterations in cognitions and mood (investigator-developed measure) were assessed. Path analysis examined associations between proposed correlates and PTSS.
Results: Among patients, higher neuroticism and greater physical symptom distress were concurrently associated with higher PTSS (Bs> 0.10, ps< .02); greater physical symptom distress at T1 and greater social support at T2 were associated with higher PTSS at T3 (Bs≥ 0.37, ps≤ .04). Among caregivers, female gender, higher neuroticism (Bs> 0.12, ps<.01), and lower social support (Bs< -0.24, ps< .02) were associated with higher concurrent PTSS; lower patient physical symptom distress and social support at T1 (Bs< -0.27, ps< .03) and longer hours spent for caregiving at T2 (B= 0.03, p=.05) were associated with higher PTSS at T3.
Conclusions: Findings identify cancer-specific risk factors (physical symptoms, hours caregiving) unique to cancer patients and caregivers, in addition to established risk factors (female, neuroticism, social support) in non-medical populations.
Implications for Cancer Survivors: The identified subgroups should receive ongoing screening and psychosocial referral for cancer-related PTSS.
Aldo-keto reductase 1C3, which contributes to skin barrier function, is downregulated by single nucleotide polymorphisms predominantly in females with early-onset psoriasis
Authors: Nojiri Yuka , Nakamura Motoki , Magara Tetsuya , Yamamoto Aya , Ikumi Kyoko , Nakamura Reiko , Nishida Emi , Haarmann-Stemmann Thomas , Morita Akimichi 
Published: November 2022

Abstract
The principal pathology of psoriasis is impaired skin barrier function, epidermal thickening, and granular layer loss. Exposure to extrinsic factors such as tobacco smoke and air pollutants is associated with the development of psoriasis. Aryl hydrocarbon receptors (AHRs) are activated by extrinsic factors associated with the development of psoriasis and act as transcriptional regulators. Expression of aldo-keto reductase (AKR) 1C3 in the epidermal spinous layer regulates epidermal keratinocyte differentiation via the AHR signaling pathway. We investigated whether single nucleotide polymorphisms (SNPs) in AKR1C3 are associated with the pathogenesis of psoriasis. The proportions of rs12529 G/C, C/C variants and rs12387 A/A, A/G variants were 2-fold higher in Japanese psoriasis patients (n = 231) compared with a Japanese healthy cohort. The SNPs were significantly more common than the majority variants in female patients with disease onset ≤ 22 years of age. Patients with rs12529 G > C and rs12387 A > G SNPs exhibited significantly lower AKR1C3 expression and higher expression of late differentiation markers. In conclusion, AKR1C3 downregulation caused by rs12529 G > C and rs12387 A > G SNPs in the epidermis induces abnormal early differentiation of keratinocytes and skin barrier dysfunction, which may contribute to the genetic pathogenesis of psoriasis in young females.
(2R,6R)-Hydroxynorketamine restores postsynaptic localization of AMPAR in the prelimbic cortex to provide sustained pain relief
Authors: JIN Tingting , Ng Hei Lui Lhotse , Jiang Yanjun , Ho Idy , Zou Yidan , Li Qian , Zhang Lin , Chen Huarong , Wang Jie , Zhou Jingying , Gin Tony , Wu William , Chan Matthew , Liu Xiaodong 
Published: November 2022

Abstract
Neuropathic pain is a difficult-to-treat pain condition that can affect patients for years. (2R,6R)-hydroxynorketamine (R-HNK) is a ketamine metabolite without dissociative effects and has been evaluated as an alternative to ketamine in chronic pain management. The mechanism of action remains elusive. Here we report that repeated systemic or contra-prelimbic cortex (PrL) infusion of R-HNK in the acute stage of nerve injury produces sustained pain relief for at least 14 days in the mouse spared nerve injury (SNI) model of neuropathic pain. Transcriptomic analysis suggests that SNI is associated with increased Brain-derived neurotrophic factor (Bdnf) signaling, abnormal dendritic spine organization, and reduced α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) activity in PrL. Activity-regulated cytoskeleton-associated protein (Arc) is identified as the top gene in the leading-edge analysis of the gene set. R-HNK administration abolishes these transcriptomic changes. Further studies confirm the transcriptome findings. Finally, we show that enhancing PrL activity by R-HNK increases PrL-periaqueductal gray (PAG) connectivity, which is essential for R-HNK-mediated pain relief. Our study highlights AMPAR suppression due to continuous Bdnf/Arc elevation in PrL as a mechanism of central sensitization after SNI. R-HNK can recalibrate Bdnf/Arc/AMPAR axis and restore PrL-PAG connectivity to induce sustained alleviation of neuropathic pain.
Decreased liver damage in rat models of short bowel syndrome through DPP4 inhibition
Authors: Sueyoshi Ryo , Furuhashi Nanami , Ishii Junya , Yamaguchi Ryusuke , Kawakami Marumi , Tanabe Kenji , Segawa Osamu 
Published: November 2022

Abstract
Purpose
Total parenteral nutrition causes liver damage in patients with short bowel syndrome (SBS), in whom intestinal failure-associated liver disease (IFALD) is the strongest risk factor for mortality. We previously demonstrated the efficacy of dipeptidyl peptidase-4 inhibitors (DPP4-Is) for nutritional absorption and intestinal barrier function enhancement. Herein, we investigated the efficacy of DPP4-Is in preventing liver damage in SBS rat models.
Methods
Rats were allocated to one of five groups: normal saline (NS) + sham, DPP4-I + sham, NS + SBS, DPP4-I + SBS, and GLP-2 + SBS. DPP4-I or NS was administered orally once daily. Serum aspartate aminotransferase, alanine aminotransferase (ALT), alkaline phosphatase, and total bile acid levels were measured to assess liver function. Moreover, we evaluated liver damage using the SAF (steatosis activity fibrosis) score, which is also used to assess nonalcoholic steatohepatitis.
Results
ALT levels and SAF scores were significantly lower in the DPP4-I + SBS group than in the NS + SBS group. Jejunal and ileal villus heights were significantly higher in the DPP4-I + SBS group than in the GLP-2 + SBS group.
Conclusions
The downregulation of ALT levels and SAF scores triggered by DPP4-I use may be correlated with DPP4-I-induced adiposis inhibition in SBS and NASH models. Therefore, DPP4-I may be used to reduce IFALD in patients with SBS.
Association of the body mass index with poor outcome at discharge in patients with aneurysmal subarachnoid hemorrhage: A registry study and literature review
Authors: Oku Shinichiro , Ikawa Fusao , Hidaka Toshikazu , Matsuda Shingo , Ozono Iori , Horie Nobutaka , Yamaguchi Shuhei 
Published: November 2022

Abstract
Although several studies have reported on the impact of the body mass index (BMI) on the functional outcome of aneurysmal subarachnoid hemorrhage (aSAH), the relationship is still unclear. Therefore, this study aimed to investigate the risk factors of poor outcome of aSAH including the BMI and summarize past reports that referred to the BMI and functional outcome after aSAH. Between 2010 and 2017 at Shimane Prefectural Central Hospital, 860 patients with aSAH were enrolled in the registry. Basic patient characteristics including the BMI, data related to aSAH, and blood samples were recorded. Multivariable logistic regression analyses were conducted to identify risk factors of poor outcome, which was defined as a modified Rankin Scale score > 2 at discharge. In addition, we summarized studies reporting the relationship between the BMI and aSAH. In 393 patients of our study, age (odds ratio, 1.10; 95% confidence interval, 1.07–1.14), both underweight (2.65; 1.23–5.73) and overweight (2.31; 1.08–4.93), World Federation of Neurosurgical Societies grade, and symptomatic vasospasm (4.17; 2.00–8.69) were risk factors for poor outcome at discharge. Compared to findings of the other past studies in our literature review, underweight association with a risk factor for poor outcome was a new finding. In conclusion, not only a higher BMI but also a lower BMI were associated with a poor outcome at discharge for patients with aSAH.
Coupling of Liquid and Surface Chemistry in Urea SCR Systems
Authors: Kuntz Christian , Jägerfeld Paul Jakob , Mmbaga Joe , Hayes Robert E. , Deutschmann Olaf 
Published: November 2022

Abstract
Close-coupled selective catalytic reduction (SCR) systems are one method to deal with tightening emission legislation for NOx in internal combustion engines. Due to smaller mixing sections and at unfavourable boundary conditions, however, urea-water solution (UWS) droplets can impact on the SCR catalyst itself. To investigate this phenomenon further, this work develops a modeling capability of this process. Established mechanism for NH3-SCR and HNCO hydrolysis from literature are integrated into DETCHEMCHANNEL and a 2D COMSOL model to simulate the influence in the SCR Channel. Simulations are validated against end-of-pipe experiments from literature and spatially resolved concentration profiles from a hot gas test rig with very good agreement. Finally, a channel simulation is coupled with a model to describe the catalytic decomposition of an urea droplet. The coupled simulation is able to simulate the influence of UWS droplet impact onto a catalyst channel. Fast droplet decomposition causes a peak in NH3 and HNCO in the single channel and thus increases NOx conversion. However, the overall uniformity and efficiency is decreased, which is why droplet impact on the catalyst should be strictly avoided.
Active Learning Entropy Sampling Based Clustering Optimization Method for Electricity Data
Authors: qingnan Wang , zhaogong Zhang 
Published: November 2022

Abstract
Clustering is a crucial part in the field of data mining, and common clustering methods include division-based methods, hierarchy-based methods, density-based methods, and grid-based methods. In order to improve the accuracy of clustering, an optimization study is made mainly for the division-based method FCM clustering, and an FCM clustering method that integrates active learning and principal component analysis (PCA) is proposed. The method first uses principal component analysis to reduce the dimensionality of the data to reduce the computation of electricity data, then trains the sample model by active learning, and introduces the entropy (Entropy) method in the uncertainty sampling method, the larger the entropy means the greater the uncertainty of the sample, and the smaller the entropy means the smaller the uncertainty of the sample, so as to filter the electricity data, and finally the electricity data are clustered by FCM clustering The power data is finally categorized by FCM clustering, and with the proliferation of power data, the power data can be more accurately categorized using this method to achieve the stability of the power grid as well as the utilization rate. Experimental results on three datasets show that this method improves the accuracy of power data clustering by up to 2 percentage points compared to the traditional clustering method without active learning, and achieves good results in each dataset compared to other methods.
Trends in Global Health Research among Universities in China: A Bibliometric Analysis
Authors: Wu Chenkai , Yan Lijing L. , Long Qian , Liu Yunguo , Tan Jie , Lou Zhexun , Tang Shenglan 
Published: November 2022

Abstract
Background: We have witnessed considerable progress in developing global health education and research in China. Nevertheless, evidence of the progress of Chinese universities’ contributions to global health research is limited. More efforts are needed to depict the progress Chinese universities have collectively made in advancing the field of global health. This study aimed to examine Chinese universities’ collective contributions to global health research by describing the longitudinal trends in global health research publications, uncovering research themes in global health, and exploring collaboration patterns.
Methods: A comprehensive bibliometric analysis was conducted for original research studies of the ten founding members of the China Consortium of Universities for Global Health, one of the largest networks of global health research and education in China.
Results: We found that (1) the number of research publications in the field of global health has steadily increased from 2014 to 2020, (2) non-communicable disease was the most popular research topic, accounting for over one-third of total publications, followed by maternal and child health and neurological and mental disorders and diseases, (3) less than one-fifth of papers involved primary data collection, with the majority of the study populations from low-income and lower-middle-income countries in Asia and Africa, and (4) a sizable collaboration network has been established with co-authors from over 200 oversea universities or organizations, with about one third from the US.
Conclusions: More concerted efforts by multiple stakeholders, including government, private sectors, funding agencies, academic institutions, and researchers, are needed to advance the development of global health research in China.