bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel classification and risk model based on ferroptosis‐related lncRNAs to predict oncologic outcomes for gastric cancer patients
Authors: Yue Qingfang , Bai Jun , Wang Fei , Xue Fei , Li Lianxiang , Duan Xianglong 
Published: March 2022

AbstractGastric cancer (GC) is a highly heterogeneous malignancy, characterized by high mortality and poor prognosis. Ferroptosis is a newly defined nonapoptotic programmed cell death mechanism that has been implicated in the development of various pathological conditions. We aimed to identify ferroptosis‐related long noncoding RNA (lncRNAs) that might be used to predict GC prognosis. The data were obtained from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus database. Two subtypes, C1 and C2, were identified, which had significant variations in prognosis and immune cell infiltrations. Differentially expressed genes between the subtypes were found to be involved in multiple tumor‐associated pathways. Subsequently, a training dataset and a testing dataset were created from the TCGA dataset. A predictive model for GC patients based on six ferroptosis‐related lncRNAs (including STX18‐AS1, MIR99AHG, LINC01197, LINC00968, LINC00865, and LEF1‐AS1) was developed. The model could stratify patients into a high‐ and low‐risk group, showing good predictive performance. The testing dataset, entire TCGA dataset, and GSE62254 cohort both confirmed the predictive value of the model. Compared to the clinical parameters (including gender, age, and grade), the risk model was an independent risk factor for GC patients. Moreover, a nomogram (containing our risk score model and clinical parameters) was constructed, which might provide great potential to improve prediction accuracy. Moreover, the single‐sample gene set enrichment analysis revealed that the high‐risk group was linked to various signaling pathways involved in the regulation of GC progression. Conclusively, a novel classification and risk model based on ferroptosis‐related lncRNAs that can predict oncologic outcomes for GC patients has been developed.
Identification of acridinedione scaffolds as potential inhibitor of DENV‐2 C protein: An in silico strategy to combat dengue
Authors: Kumar Sachin , Bhardwaj Vijay K. , Singh Rahul , Das Pralay , Purohit Rituraj 
Published: March 2022

AbstractDengue is a prominent viral disease transmitted by mosquitoes to humans that affects mainly tropical and subtropical countries worldwide. The global spread of dengue virus (DENV) is mainly occurred by Aedes aegypti and Aedes albopictus mosquitoes. The dengue virus serotypes‐2 (DENV‐2) is a widely prevalent serotype of DENV, that causes the hemorrhagic fever and bleeding in the mucosa, which can be fatal. In the life cycle of DENV‐2, a structural capsid (DENV‐2 C) protein forms the nucleocapsid assembly and bind to the viral progeny RNA. For DENV‐2 maturation, the nucleocapsid is a vital component. We used virtual ligand screening to filter out the best in‐house synthesized acridinedione analogs (DSPD molecules) that could efficiently bind to DENV‐2 C protein. The molecular docking and dynamics simulations studies were performed to analyze the effect of DSPD molecules on DENV‐2 C protein after binding. Our findings showed that DSPD molecules strongly interacted with DENV‐2 C protein, as evident from molecular interactions and several time‐dependent molecular dynamics‐driven analyses. Moreover, this study was also supported by the thermodynamic binding free energy and steered molecular dynamics simulations. Therefore, we intend to suggest that the DSPD3 molecule could be used as a potential therapeutic molecule against dengue complications as compared to the cocrystallized inhibitor ST‐148. However, further studies are required to demonstrate the ability of DSPD3 to induce DENV‐2 C tetramer formation.
Analytical and clinical evaluation of the light‐initiated chemiluminescent assays for measurement of human thyroid hormones
Authors: Li Jing , Huang Youyuan , Xiang Guiming , Xu Enjun , Chen Tao , Yang Ming , Zhang Jie 
Published: March 2022

AbstractBackgroundLight‐initiated chemiluminescent assay (LiCA) is a new homogeneous immunoassay. The aim of this study was to evaluate the analytical and clinical performance of the assays for the detection of thyroid hormones based on the fully automated LiCA 800 analyzer.MethodsAnalytical validations of the LiCA thyroid assays (TSH, FT3, FT4, T3, and T4) included precision, linearity, analytical sensitivity, interference, and method comparison applying the protocols of the Clinical and Laboratory Standards Institute (CLSI). The diagnostic performance was assessed by the receiver operating characteristic (ROC) curve analysis with different assay schemes for the diagnosis of hyperthyroidism and hypothyroidism.ResultsWithin‐run and within‐lab precisions (%CV) of the five assays ranged from 1.06 to 6.40% at all concentrations evaluated. A satisfactory linearity was verified over the entire measuring range for TSH, T3, and T4 (R > 0.99, change in recovery <10%, p = 0.000 all). Paired‐comparison measurements presented a comparable assay for each of the five assays (R > 0.96, median bias <5%, p < 0.0001 all) between LiCA and Cobas across three institutes. The diagnostic accuracy of the LiCA assays for hyperthyroidism or hypothyroidism was quantified by the areas under curves (AUC) as 0.925 or 0.832 with the five‐assay panel (TSH, FT3, FT4, T3, and T4) and as 0.921 or 0.811 with the three‐assay panel (TSH, FT3, and FT4), respectively. No significant difference was found between the AUC of LiCA and that of DxI, Cobas, or Centaur (p > 0.3 all).ConclusionLiCA 800 provides a precise and high‐throughput immunoassay platform for detection of thyroid hormones. It is acceptable for clinical use.
The role of miRNAs as a big master regulator of signaling pathways involved in lymphoblastic leukemia
Authors: Bagheri Malihe , Sarabi Parisa Zia , Mondanizadeh Mahdieh 
Published: March 2022

AbstractMicroRNAs (miRNAs) belong to small noncoding RNAs, which have long attracted researchers' attention because of their potency in acting either as oncogenes or tumor‐suppressors in cancers. acute lymphocytic leukemia (ALL) and chronic lymphocytic leukemia (CLL) are two known types of leukemia with high mortality rates in adults and children. On a molecular basis, various signaling pathways are active in both types, making researchers consider the potential role of miRNAs in activating or suppressing these pathways to further hinder cancer development. In this review, we summarized the potential miRNAs, especially circulating ones, involved in essential signaling pathways in the ALL and CLL patients which serve as biomarkers and valuable targets in the treatment fields.
Polygenic mechanisms underpinning the response to exercise‐induced muscle damage in humans: In vivo and in vitro evidence
Authors: Baumert Philipp , Cocks Matthew , Strauss Juliette A. , Shepherd Sam O. , Drust Barry , Lake Mark J. , Stewart Claire E. , Erskine Robert M. 
Published: March 2022

AbstractWe investigated whether 20 candidate single nucleotide polymorphisms (SNPs) were associated with in vivo exercise‐induced muscle damage (EIMD), and with an in vitro skeletal muscle stem cell wound healing assay. Sixty‐five young, untrained Caucasian adults performed 120 maximal eccentric knee‐extensions on an isokinetic dynamometer to induce EIMD. Maximal voluntary isometric/isokinetic knee‐extensor torque, knee joint range of motion (ROM), muscle soreness, serum creatine kinase activity and interleukin‐6 concentration were assessed before, directly after and 48 h after EIMD. Muscle stem cells were cultured from vastus lateralis biopsies from a separate cohort (n = 12), and markers of repair were measured in vitro. Participants were genotyped for all 20 SNPs using real‐time PCR. Seven SNPs were associated with the response to EIMD, and these were used to calculate a total genotype score, which enabled participants to be segregated into three polygenic groups: ‘preferential’ (more ‘protective’ alleles), ‘moderate’, and ‘non‐preferential’. The non‐preferential group was consistently weaker than the preferential group (1.93 ± 0.81 vs. 2.73 ± 0.59 N ∙ m/kg; P = 9.51 × 10−4) and demonstrated more muscle soreness (p = 0.011) and a larger decrease in knee joint ROM (p = 0.006) following EIMD. Two TTN‐AS1 SNPs in linkage disequilibrium were associated with in vivo EIMD (rs3731749, p ≤ 0.005) and accelerated muscle stem cell migration into the artificial wound in vitro (rs1001238, p ≤ 0.006). Thus, we have identified a polygenic profile, linked with both muscle weakness and poorer recovery following EIMD. Moreover, we provide evidence for a novel TTN gene‐cell‐skeletal muscle mechanism that may help explain some of the interindividual variability in the response to EIMD.
Roles of nutrition in muscle health of community‐dwelling older adults: evidence‐based expert consensus from Asian Working Group for Sarcopenia
Authors: Chen Liang‐Kung , Arai Hidenori , Assantachai Prasert , Akishita Masahiro , Chew Samuel T.H. , Dumlao Lourdes Carolina , Duque Gustavo , Woo Jean 
Published: March 2022

AbstractGeneral muscle health declines with age, and in particular, sarcopenia—defined as progressive loss of muscle mass and strength/physical performance—is a growing issue in Asia with a rising population of community‐dwelling older adults. Several guidelines have addressed early identification of sarcopenia and management, and although nutrition is central to treatment of sarcopenia, there are currently few guidelines that have examined this specifically in the Asian population. Therefore, the Asian Working Group for Sarcopenia established a special interest group (SIG) comprising seven experts across Asia and one from Australia, to develop an evidence‐based expert consensus. A systematic literature search was conducted using MEDLINE on the topic of muscle health, from 2016 (inclusive) to July 2021, in Asia or with relevance to healthy, Asian community‐dwelling older adults (≥60 years old). Several key topics were identified: (1) nutritional status: malnutrition and screening; (2) diet and dietary factors; (3) nutritional supplementation; (4) lifestyle interventions plus nutrition; and (5) outcomes and assessment. Clinical questions were developed around these topics, leading to 14 consensus statements. Consensus was achieved using the modified Delphi method with two rounds of voting. Moreover, the consensus addressed the impacts of COVID‐19 on nutrition, muscle health, and sarcopenia in Asia. These statements encompass clinical expertise and knowledge across Asia and are aligned with findings in the current literature, to provide a practical framework for addressing muscle health in the community, with the overall aim to encourage and facilitate broader access to equitable care for this target population.
Virtual reality approach for orthodontic education at School of Stomatology, Jinan University
Authors: Huang Yue , Cheng Xin , Chan Unman , Zheng Liyang , Hu Yingwen , Sun Yueting , Lai Pengyu , Dai Jun , Yang Xuesong 
Published: March 2022

AbstractObjectivesThe development of virtual reality (VR) has exerted an enormous impact on higher education. However, the VR application in dental education is still at an early stage in China. This study explored the usability of a VR application in training the practical skills of dental students.MethodsWe first utilized the “system usability scale” (SUS) to verify the validity of the VR application in this study. Next, the VR training on orthodontic bracket bonding was experienced by the participants. The subsequent survey was delivered to collect the participants’ perception and evaluation of the VR system application in training the practical skills of prospective dentists.ResultsThe SUS score was 76.17 ± 9.89, suggesting an above‐average evaluation of the system's usability and maturity. The response from the questionnaire supplied relatively positive responses to the VR system application on “user experiences,” “perceived usefulness of VR application on orthodontics,” and “perceived ease of manipulation.” The male students exhibited strong interest in manipulating the VR system and were more capable of manipulating the VR system than the female students. VR experience did not affect the participants’ responses to the questions.ConclusionsOverall, the use of VR in dental education achieved the expected outcomes. Most students identified the VR training as an enjoyable learning process, and it could be repetitively experienced without further costs, which might have potential positive effects on long‐term learning outcomes, although there is room to further improve the effectiveness evaluation of VR.
Modulatory effect of IL‐1 inhibition following lipopolysaccharide‐induced neuroinflammation in neonatal microglia and astrocytes
Authors: Pierre Wyston C. , Londono Irène , Quiniou Christiane , Chemtob Sylvain , Lodygensky Gregory A. 
Published: March 2022

AbstractIntroductionInflammation‐induced white matter injury (WMI) in preterm infants is characterized by microglia activation, astrogliosis, oxidative stress and neurodevelopmental impairments. Microglia and astrocytes activation can be described under a broad spectrum of activation profile with extremes described as pro‐inflammatory/neurotoxic (M1 microglia or A1 astrocyte) or anti‐inflammatory/neuroprotective (M2 microglia or A2 astrocyte) in response to stimuli including lipopolysaccharide (LPS) and interleukin 1 (IL‐1). As IL‐1 signalling pathway has been posited as a major driver of inflammation‐induced perinatal WMI, our aim was to evaluate the contribution of IL‐1 modulation in LPS‐induced microglia and astrocyte activation.MethodsPrimary neonatal cell co‐cultures of astrocytes and microglia were treated with LPS (100 ng/ml) for 8 h or 24 h. Two distinct IL‐1 receptor antagonists, Rytvela or Kineret (1 μg/ml), were added simultaneously with LPS to respectively modulate or block IL‐1 receptor. Medium was collected to measure levels of IL‐1β. Expression of markers related to pro‐ and anti‐inflammatory microglia and astrocyte activation profiles and antioxidant genes were assessed.ResultsAt 8 h, LPS exposure induced pro‐ (M1/A1) and anti‐inflammatory (M2/A2) marker expression and inhibited antioxidant gene expression in microglia and astrocytes. By 24 h, continuous LPS exposure increased pro‐inflammatory and neurotoxic microglial and astrocytic markers (M1/A1), as well as antioxidant genes. Administration of IL‐1 antagonists Rytvela and Kineret with continuous LPS exposure had no significant effect on modulation of specific microglia and astrocyte activation pathways.Discussion/ConclusionWe show that LPS effects on in vitro neonatal microglia and astrocytes co‐cultures are time dependent eliciting a number of pro‐ and anti‐inflammatory responses during the acute phase of inflammation (8 h), which shift towards pro‐inflammatory and neurotoxic factors by 24 h. Although LPS‐induced inflammation led to abundant IL‐1 expression, IL‐1 inhibition had no significant impact on in vitro modulation of microglia and astrocyte activation pathways towards M2 and A2 activation profile.
Interleukin‐18 mediated inflammatory brain injury after intracerebral hemorrhage in male mice
Authors: Li Hao , Tian Jingluan , Yin Yin , Diao Shanshan , Zhang Ximeng , Zuo Tao , Miao Zhigang , Yang Yi 
Published: March 2022

AbstractInterleukin‐18 (IL‐18), a pro‐inflammatory cytokine, is thought to be associated with inflammation in many neurological diseases such as ischemic stroke and poststroke depression, but the role of IL‐18 in inflammatory injury after intracerebral hemorrhage (ICH) remains unclear. In this study, we established the ICH model in male mice and found that IL‐18 expression including protein and mRNA levels was significantly increased in brain tissues after ICH. Meanwhile, exogenous IL‐18 exacerbated cerebral hematoma and neurological deficits following ICH. In the IL‐18 knockout group, the size of hematoma and neurological functions after ICH was decreased compared with the wild‐type group, suggesting the critical role of IL‐18 on the modulation of brain injury after ICH. Importantly, exogenous IL‐18 increased microglial activation in brain tissues after ICH. Furthermore, IL‐18 knockout resulted in the reduction of activated microglia after ICH. These results indicated that IL‐18 may regulate the inflammatory response after ICH through the activation of microglia. Thus, IL‐18 is expected to be a promising therapeutic target for secondary brain injury after ICH.
Two spatial types of North China heatwaves and their possible links to Barents‐Kara Sea ice changes
Authors: Zhang Guwei , Zeng Gang , Yang Xiaoye , Iyakaremye Vedaste 
Published: March 2022

AbstractHeatwaves of different spatial types can cause various impacts. Based on the spatial heatwave intensity, 47 North China heatwaves from 1961 to 2019 could be classified into two categories: the S‐type with a centre in the south and the N‐type in the north. There were more N‐type events (36) than the S‐type (11). Observational analysis indicated that under atmospheric circulations favourable to North China heatwaves (e.g. the enhanced continental high), there is a linkage between the sea ice concentration (SIC) changes in the Barents‐Kara Sea (BKS) and these two types of heatwaves. Relative to high BKS SIC, the reduction of SIC would strengthen the cyclonic circulation over the Siberian‐BKS, which could coordinate with the continental high to enhance the cyclonic over the Jianghuai region. A cyclonic‐anticyclonic‐cyclonic pattern is present over the BKS‐North China‐Jianghuai. The cyclonic circulation over the Jianghuai region would push the continental high northerly, resulting in the heatwave centre to the north. It would also cause favourable conditions for precipitation over southern North China, reducing temperatures and raising soil moisture, not conducive to the heatwave. Therefore, the heatwave intensity in southern North China would weaken and the heatwave centre would locate in the north, making the N‐type. Conversely, when the BKS SIC increases, the upper cyclonic over the Siberian‐BKS would not get strengthened, indicating entire North China would be controlled by continental high, and the heatwaves would be southerly relative to the N‐type, namely the S‐type. A series of numerical experiments using NCAR CAM5.3 confirm the above observational results, whereby if the BKS SIC reduces (increases), the North China heatwave is likely to be the N‐type (S‐type).
Orange‐fleshed sweetpotato production: Progress and perspectives for value chain development in West Africa
Authors: Sohindji Fernand S. , Adje O. A. Charlotte , Fassinou Hotegni V. Nicodème , Fanou Fogny Nadia , Akponikpe Tania , Quenum Florent , Achigan‐Dako Enoch G. 
Published: March 2022

AbstractAfrica and Asia bear the greatest cases of vitamin A deficiency whereas Orange‐Fleshed Sweetpotato (OFSP) as a possible solution to vitamin A deficiency, brings income, food and nutritional security for smallholders involved in its production. Reports indicated low adoption of OFSP in West Africa due to poor value chains and wide adoption of White‐Fleshed Sweetpotato (WFSP). Here, we reviewed OFSP interventions and programmes undertaken in West Africa, identified constraints limiting OFSP production, and updated the required actions for OFSP value chains development in West Africa. We reported 10 significant interventions and 20 released OFSP varieties within 4 countries. The main bottlenecks to OFSP value chains development are the lack of improved cultivars exhibiting high and stable yield potential, predominance of WFSP, non‐exposition of whole population to OFSP varieties, limited number of improved OFSP varieties available, and inadequacy of improved OFSP varieties to smallholder preferences. The roles of donors, research institutions, local partners, and smallholder's contributions in increasing OFSP production and consumption are provided. This will fuel the development of strong OFSP value chains in West Africa.
Effects of fructooligosaccharides (FOS) on the composition of cecal and fecal microbiota and the quantitative detection of FOS‐metabolizing bacteria using species‐specific primers
Authors: Gu Jiayu , Cui Shumao , Tang Xin , Liu Zhenmin , Zhao Jianxin , Zhang Hao , Mao Bingyong , Chen Wei 
Published: March 2022

AbstractBACKGROUNDFructooligosaccharides (FOS) are a kind of prebiotic. Previous studies concerning the effect of FOS on intestinal microbiota have focused on Bifidobacterium and Lactobacillus. However, the presence of other FOS‐utilizing bacteria makes it necessary to investigate the quantitative changes in these bacterial species in the intestine after FOS intake. In this study, the composition of cecal and fecal microbiota was analyzed using MiSeq sequencing, and the abundance of FOS‐utilizing bacteria was detected using quantitative polymerase chain reaction after the oral administration of FOS.RESULTSSpecies‐specific primers for FOS‐utilizing bacteria were designed with superior amplification efficiency for quantification. After FOS intervention, the relative abundance of Bifidobacterium pseudolongum in feces increased to 17.37% and the abundance reached 2.28 × 1010 CFU g−1. The abundance of Bifidobacterium longum and Bifidobacterium breve did not change significantly. Whereas the abundance of Ligilactobacillus murinus decreased, that of Lactiplantibacillus plantarum, Lacticaseibacillus paracasei, and Lacticaseibacillus rhamnosus remained at approximately 104 CFU g−1.CONCLUSIONSpecies‐specific primers for FOS‐utilizing bacteria were successfully developed, and we confirmed that FOS significantly increased the relative abundance and the abundance of B. pseudolongum in mice, while decreasing the proportion of Lactobacillus. The detection of these species using 16S ribosomal DNA sequencing and quantitative polymerase chain reaction showed the same results. Further investigations are needed to reveal the response of the intestinal microbiota to different FOS compositions. These techniques will contribute to future studies about the composition and dynamics of the intestinal microflora. © 2022 Society of Chemical Industry.
Simultaneous determination of dimethoate and its metabolite omethoate in curry leaf using LC‐MS/MS and risk assessment
Authors: Pasar Ratnamma , MS Pallavi , R Harischandra Naik , M Devaraj , P Nandini , M Bheemanna , R Badariprasad P , M Paramasivam 
Published: March 2022

This study presents the method development, validation, and simultaneous determination of dimethoate and its metabolite omethoate in curry leaf. Samples were extracted following modified quick, easy, cheap, effective, rugged, and safe extraction protocol and analyzed using liquid chromatography‐tandem mass spectrometry. The limit of quantification in the matrix was 0.005 μg g−1 for dimethoate and omethoate. Extraction using acetonitrile recorded the average recoveries in the range of 82.25 to 112.97% for dimethoate and 85.57 to 107.22% for omethoate at 0.005, 0.025 and 0.050 μg g−1 fortification levels and relative standard deviation less than 5%. Similarly, the relative standard deviation values for intraday (Repeatability) and interday (Reproducibility) tests were less than 15%. Dissipation kinetics of dimethoate 30% emulsifiable concentrate at 200 and 400 g a.i h−1 recorded initial deposits of 5.20 and 10.05 μg g−1 and 0.33 and 0.48 μg g−1 for dimethoate and omethoate, respectively, and half‐life of 3.07 and 3.34 days. The estimated hazard index value found more than one at a day after dimethoate application. It is not safe for consumer health to use curry leaves in the initial days after application.