bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Sub-Healthy Status (Syndrome): A Severe Pre-warning for Working Adults’Health in China
Authors: Xu Mengqing , Sun Ke , Yin Huihui , Ma Miao Miao , Zhang Dan Dan , Yue Wen , Ma Ya Jing , Wang Kui , Zhang Wen Jie 
Published: August 2022

Abstract
Background: We examine fasting blood metabolites related to the liver function among individuals participating in routine physical examinations.Aim: To investigate their possible“molecular sub-healthy”syndromes defined by having abnormal rise blood metabolites without relevant clinical manifestations or diagnosis, and try to use the median to define the high-risk and low-risk sub-healthy population.Design and setting:62,758 individuals of Han Chinese attending our healthy checkup clinic in the First Affiliated Hospital, School of Medicine, Shihezi University, spanning from from January 2013 to April 2018.Method: Peoples were tested for ten blood metabolites related to the liver function after fasting overnight, using an automated bioanalyzer. Results: (1) IBIL+TBIL (3858, 9.9%) had the highest number of high-risk individuals at the same time, followed by DBIL+IBIL (3525, 9.0%), DBIL+TBIL (3458, 8.9%). The high-risk combination of three indicators at the same time was DBIL+IBIL+TBIL.(3201, 8.2%). (2) The number of individuals with coexistence of three abnormal rise related to liver functions (6,753, 10.76%) or medium-defined High-risk bio-molecules(3,144, 5.01%) was higher than with coexistence of two abnormal rise(3,524, 5.62%) or high risk indicators(2,156, 4.09%). Meanwhile, the number of individuals with coexistence of multiple abnormal rise and High-risk bio-molecules were both subject to the distribution of the zero-inflated Poisson (ZIP) model.Conclusions: We proposed for the first time that Molecular Sub-Healthy Status and syndrome(MSHS1, MSHS2), MSHS1 indicates an individual having at least two function-related bio-molecules beyond normal ranges without obvious clinical symptoms. MSHS2 indicates an individual having three or more function-related bio-molecules beyond normal ranges without obvious clinical symptoms.
Ellagic Acid Alleviates Mice intestinal ischemia-reperfusion injury: A Study Based on Transcriptomics Combined with Functional Experiments
Authors: Fan Shuyuan , Xu yue , Li kun , Li bin , Diao yunpeng 
Published: August 2022

Abstract
Background
It has been reported that intestinal ischemia-reperfusion injury (IIRI) is closely related to inflammatory response, apoptosis and oxidative stress. Ellagic acid (EA) has been proved to have antioxidant and anti-inflammatory effects, and can inhibit tumor angiogenesis. The purpose of this study was to investigate the protective effects of EA on IIRI in mice.
Methods
A mouse model of IIRI was established by clamping the mesenteric artery. Effects and mechanisms of EA on IIRI were investigate by transcriptomics combined with functional experiments.
Results
The symptoms of IIRI were reflected in significant increases in inflammatory factors such as TNF-α and IL-1β; significant increases in oxidative stress indicators such as MDA and GSH and decreases in SOD and promotion of the apoptotic protein Bax/Bcl-2. These indicators were significantly alleviated by EA. And after EA treatment, transcriptomics results identified AKT1 differentially expressed mRNAs mainly enriched in PI3K/AKT signalling pathway.
Conclusion
This study illustrates the protective effects against IIRI, the possible mechanisms were also studied. This study provides new scientific information for the application of EA in IIRI therapy.
Integrated analysis identified prognostic microRNAs in breast cancer
Authors: Shang Cong , Chen Qingyan , Zu Fuqiang , Ren Weidong 
Published: August 2022

Abstract
MicroRNAs (miRNAs) play pivotal roles in the formation and progression of breast cancer (BC). Here, we attempt to identify miRNAs associated with BC prognosis and progression through integrated analysis. We firstly screened out 83 differentially expressed miRNAs (DEMs) in 1249 BC samples and 151 normal samples. Then, we validated their roles in expression and prognosis, identified 2 survival-related DEMs, and established a risk model. The prediction efficiency was assessed both in the training group and the validation group. ROC curve indicated a good prediction ability with 1, 3, and 5 years survival of 0.73, 0.72, and 0.72, respectively. Moreover, hsa-miR-127 was found as an independent prognostic factor for BC. Functional analyses revealed it is involved in various cancer pathways, such as the PI3K-Akt pathway and p53 pathway. Additionally, tissue and cell experiments were conducted to verify the regulatory effect of miR-127 in BC. The expression of miR-127 was down-regulated both in BC tissue and cell lines. The interference or overexpression of miR-127 was significantly increased or decreased BC cell proliferation, invasion, and migration, respectively. And this effect of miR-127 was consistent with tumorigenic ability and tumor volume in nude mice. These findings indicated that low expression of miR-127 contributes to BC migration, invasion, and tumorigenesis, and suggested a therapeutic target and prognostic biomarker for BC.
“Nutripiatto”: a tool for nutritional education. A survey to assess dietary habits in preschool children.
Authors: Lattanzi Greta , Rosa Claudia Di , Sacco Roberto , Spiezia Chiara , Cattafi Samanta , Romano Leonardo , Benvenuto Domenico , De Gara Laura , Khazrai Yeganeh Manon 
Published: August 2022

Abstract

Purpose
Childhood obesity is a worldwide public health issue associated with metabolic and psychological comorbidities. There is increasing evidence that shows that children’s lifestyle habits are shifting towards obesity with dire consequences on their future well-being and on healthcare costs. Given that, the purpose of the present pilot study is to evaluate the effectiveness of "Nutripiatto" as a tangible tool for food education to help children and their caregivers reduce portion sizes and improve food choices.
Methods
We enrolled 108 children aged 4–6 years (53% female and 47% male) to perform nutrition education interventions to improve children’s dietary habits and introduced Nutripiatto, a visual plate icon, combined with an easy guide, that was used by children during the intervention. Children’s dietary habits were investigated using a Food Frequency Questionnaire at baseline and at the end of the study, following the use of Nutripiatto for one month.
Results
Children significantly increased portion sizes and frequency of consumption of vegetables, while reduced those of several foods, reaching the recommended dietary allowances and frequency of consumption. Daily consumption of water significantly increased reaching the suggested amount.
Conclusions
On the basis of our results, Nutripiatto can be considered an effective visual guide and a helpful tool to achieve small changes and empower families towards healthier food. It can be also considered an effective educational tool useful for nutritionists and health care professionals in improving children’s dietary behaviors.
Temporal Localized Turing Patterns in Mode-Locked Semiconductor Lasers
Authors: Bartolo Adrian , Vigne Nathan , Marconi Mathias , Beaudoin Grégoire , Pantzas Konstantinos , Sagnes Isabelle , Huyet Guillaume , Maucher Fabian , Gurevich Svetlana , Javaloyes Julien , Garnache Arnaud , Giudici Massimo 
Published: August 2022

Abstract
We show that large aspect-ratio Vertical External-Cavity Surface-Emitting Lasers (VECSELs) with a saturable absorber can be operated in the regime of spatio-temporal mode locking. The emitted pulses exhibit a spatial profile resulting from the phase locking between an axial plane-wave with a set of tilted waves having a hexagonal arrangement in the Fourier space. We show that these pulsating patterns are temporally localized, i.e. they can be individually addressed by modulating the optical pump.The theoretical analysis shows that the emergence of these pulsating patterns is a signature of a Turing instability whose critical wave vector depends on spherical aberrations of the optical elements. Our result reveals that large aspect-ratio VECSELs offer unique opportunities for studying fully developed spatio-temporal dynamics.
The Behavior of COVID-19 in urban health among the capitals of Rio de Janeiro and Belo Horizonte through the dynamics of the SEIR model
Authors: SOUZA ERLAINE DA SILVA , COHEN SIMONE CYNAMON , BRUSTOLINI OTÁVIO JOSÉ BERNARDES 
Published: August 2022

Abstract
Objective: To describe the dynamics of the Covid-19 pandemic associated with urban health in the capitals of Belo Horizonte and Rio de Janeiro using a mathematical model based on ordinary differential equations in the susceptible, exposed, infected, and removed SEIR dynamics model. Study design: This is a search for data in the Department of Informatics of the Unified Health System and data available and published online by the health secretariats of the capitals of Belo Horizonte and Rio de Janeiro.Methods: A search was carried out between February 2019 and May 2020 between territorial area, mobility, sanitation, health information (TABNET), and information from the health secretariat of the capitals of Rio de Janeiro and Minas Gerais. It was used the SEIR differential equation model by the R program. Result: The estimated population (2021) of Rio de Janeiro is 6,775,561 people, with a population density equal to 5,265.82 inhabitants per km2. In Belo Horizonte, the population is 2,530,701 people, with a population density equal to 7,167.00 inhabitants per km2. The number of COVID-19 cases in 2020 in Rio de Janeiro was 221,480, there were 18,962 deaths, and the case fatality rate was 8.7%. In Belo Horizonte, according to the epidemiological bulletin. Conclusion: The SEIR model helped understand the urban health of Belo Horizonte and Rio de Janeiro about COVID-19. It demonstrated that the probability of transmission would be greater among the susceptible exposed to the infection, leading to the highest number of mortalities in an urban environment.
Numerical function optimization of improved artificial bee colony algorithm and its application in finite element model modification
Authors: Tang Yu , Yue Jie , Xu Min 
Published: August 2022

Abstract

An improved artificial bee colony algorithm is proposed in this paper to improve the problems of strong exploration ability, weak exploitation ability and being easy to fall into local optimum of the basic artificial bee colony algorithm. The quality of the initial solution is improved by adopting the principle of equally spaced random distribution in the initialization phase. In the stage of employed bee and onlooker bee, it introduces the two-way search strategy and the adaptive decision factor γ, which also changes the search step length at the same time to balance algorithm exploration and exploitation capabilities by replacing the original search strategy. fifteen benchmark test functions were used to verify the optimal search ability of the improved artificial bee colony algorithm, and the improved algorithm was successfully applied to the finite element model modification. The results show that the improved algorithm can effectively improve and balance the exploitation and exploration ability of the algorithm, and show excellent global search ability. At the same time, the improved algorithm has higher correction efficiency and better applicability and practicability in the finite element model modification.
Metasurface-enabled smart wireless attacks at the physical layer
Authors: Wei Menglin , Zhao Hanting , Galdi Vincenzo , Li Lianlin , Cui Tie Jun 
Published: August 2022

Abstract
Information security is of paramount importance in the modern society, and it is crucial that communication systems are conceived and implemented in order to be inherently resilient in this respect. In current wireless communication systems, some of the most sophisticated attack strategies aim at the physical layer, i.e., at the electromagnetic wave signal carrying the information, but the implied physical interaction with the target inherently leaves traces in the physical environment, which render these attacks typically detectable. However, this may not be the case for future (the 6th generation and beyond) wireless networks, whose current vision relies on the concept of “smart radio environment”, empowered by suitably engineering reflecting devices (also called as metasurfaces) that can manipulate the wave signals in unconventional fashions (e.g., non-specular reflections) and that can be reconfigured at will. These metasurface elements, which should be pervasively deployed and suitably disguised in the indoor and outdoor environment, potentially introduce new vulnerabilities to the physical-layer attacks that should be fully understood and addressed. To this aim, here, we put forward the concept of smart wireless attacks at the physical layer by exploiting the unique capabilities of programmable metasurfaces in the joint manipulations of radio waves and digital information in a wireless scenario. Specifically, we illustrate both passive and active operational modes. In the passive mode, an attacker is capable of eavesdropping and breaking the target wireless information transfer by controlling the programmable metasurface, without radiating any signal actively. In the active mode, an attacker can not only eavesdrop but also furtively falsify the target wireless communications by sending some deceptive information to the target. In both operational modes, the detectability of the attacker can be minimized. As a proof of concept, we design and realize an attacker prototype working in the Wi-Fi band around 2.4GHz, and demonstrate experimentally its ability to hack wireless data streams. Our results raise awareness on the new types of security threats and challenges that the next-generation wireless networks will likely have to face, and indicate that suitable mitigation strategies and specific security protocols need to be conceived and developed at the present stage, while the smart-radio-environment concept is still in its infancy.
Barley (Hordeum vulgare L.) β-Tubulin gene HvTUBULIN8 has pleiotropic effects on spike and leaf development
Authors: Qin Dandan , Liu Gang , Liu Rui , Wang Chunchao , Xu Fuchao , Xu Qing , Ling Yu , Dong Guoqing , Peng Yanchun , Ge Shuangtao , Guo Ganggang , Dong Jing , Li Chengdao 
Published: August 2022

Abstract
Barley lateral spikelet morphology and grain shape play a key role in grain physical quality and yield. Several genes and QTLs for these traits have been cloned or fine mapped previously. Here, we report the phenotypic and genotypic analysis of a barley mutant with Round Lateral Spikelet (rls) from c.v. Edamai 934. The mutant had round lateral spikelet, short but round grain, shortened awn, thick glume and dark green leaves. Histocytologic and ultrastructural analysis revealed that the difference of grain shape of rls was caused by change of cell arrangement in glume, and the dark leaf color resulted from enlarged chloroplast. HvTUBULIN8 (HvTUB8) was identified as the functional gene for rls by combination of RNA-seq, map-based cloning, virus-induced-gene-silencing (VIGS) and protein subcellular location. A single G-A substitution in exon of HvTUB8 resulted in change of Cysteine to Tyrosine. Furthermore, the mutant isoform of HvTUB8 could be detected in both nucleus and cytoplasm, whereas the wild was only in cytoplasm of wheat protoplasts. Being consistent with the rare phenotype, the “A” allele of HvTUB8 was only detected in rls, but not in a worldwide barley germplasm panel consisting about 400 accessions. VIGS confirmed that HvTUB8 was essential to maintain spike integrity. RNA-sequencing results suggest that HvTUB8 control spike morphogenesis via ethylene homeostasis and signaling, and control leaf color through chlorophyll metabolism. Collectively, our results demonstrated that HvTUB8 has pleiotropic effects on barley spike and leaf morphology and provided insight of a novel mechanism of HvTUB8 in spike and leaf development.
Heavy metal lead(Pb) removal in contaminated soils using citric acid and malic acid as washing agents
Authors: Zhao Manqing , Yan Huan , Liu Chao , Li Cong , Li Shijie , Gao Huanfang 
Published: August 2022

Abstract
Soil washing is a rapid and efficient remediation technique for soil contaminated by heavy metals. In this study, Pb was removed from contaminated soil with citric acid (CA) and malic acid (MA). The effects of washing concentration, liquid-solid(L/S) ratio, washing time, and pH value on the removal rate and metal stability in lead-contaminated soil were investigated. Results showed that the mixed chelating agent's (MC) removal rates of the heavy metal lead from the soil by citric acid (0.3m) and malic acid (0.3m) at a certain volume ratio of 4:1 (CA: MA = 4:1) was up to 88.65%. After washing, the leaching concentration of lead in soil decreased by 63.39%, which was lower than the identification standard value of hazardous waste. The speciation analysis study indicated that the acid-soluble and reducible fractions in the soil with excellent bioavailability after MC washing were reduced by 83.23% and 71.99%, respectively. Which reduces the environmental risk greatly. Besides, heavy metal lead was also found to become more stable after the soil was washed by MC. This method is shown to help reduce the bioavailability and leachability of lead and has proven to environmentally friendly way to remediate lead-contaminated soil.
Factors affecting the acceptance of electronic banking services among individuals in Yemen
Authors: GHALLAB HAMDAN MUTAHAR HAMOOD , ZHU Huiming 
Published: August 2022

Abstract
The focus of this research is to determine the factors that influence individuals' acceptance of electronic banking services offered by Yemeni banks. Therefore, our proposed study has four salient variables: security and privacy, perceived risks and benefits, website usability, and electronic banking awareness. The measurement model was created using Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA). Structural equation modeling (SEM) was used to determine the extent to which individuals accepted electronic banking services. Data were collected through a questionnaire completed by 684 participants. The results of our study found that perceived risks and benefits, website usability and electronic banking awareness directly affected the acceptance of electronic banking services by individuals. However, security and privacy did not affect acceptance of electronic banking services in Yemen. This study enables us to understand the factors that influence the acceptance of electronic banking services by individuals in Yemen. Rather than focusing on adopting electronic banking services in developed countries, as many existing studies have done, our proposed model attempts to fill this gap. Similarly, it would be applicable to other developing countries. Decision makers in banks can focus on raising awareness of electronic banking services and making individuals aware of their benefits and ease of dealing with them by promoting and advertising the advantages of these services. This will enhance the acceptance of electronic banking services by Yemeni individuals. The study recommends that understanding the factors affecting acceptance of electronic banking is essential for practitioners looking for new ways of banking.
Prevalence and factors associated with latent tuberculosis infection among residents of a monastery situated in a high-TB burden area: A cross sectional study, Sikkim, India
Authors: Siddiqui Mohammad K. , Khan Shagufta , Bhutia Rinchenla , Nair Vivek , Gurung Nirmal , Yamphel Tseten , Dadul Peggy K. , Kerongi Debya S. , Pradhan Jagat , Khati Kabita , Nair Sreenivas A. , Mannan Shamim , Rade Kiran K. , Gupta Dinesh , Malhotra Pawan , Dhawan Shikha , Mohmmed Asif 
Published: August 2022

AbstractBackground:Diagnosis and treatment of Latent Tuberculosis Infection (LTBI) remains to be one of the main bottlenecks in eradication of tuberculosis (TB). TB and LTBI risk among the residents of a congregate facility in a monastery, situated in a high-TB burden area, Sikkim, India, may be high due to their frequent travel history and has never been illustrated.Method:A population based cross sectional screening of all the monks and residents of Rumtek Monastery (Sikkim, India) was carried out for diagnosis of active TB and LTBI. TrueNat MTB and GenXpert MTB/Rif systems were utilized for active TB diagnosis, whereas QFT-plus IGRA analysis was carried out for LTBI detection. LTBI positive cases were followed up with TrueNat MTB system to diagnose any progression to active TB.Results:Among the 350 residents of the monastery, no participant was found to be having active TB infection; however, ~45% of residents were LTBI positive showing high exposure of disease to the monks belonging to various age groups (9-73 yrs). Participants with frequent travel history, family history of TB or having contacts with TB patients, showed higher percentage of LTBI. Similarly, abnormal BMI showed significant positive correlation with LTBI.Conclusion:This study provides status of high prevalence of LTBI among the residence of a congregate facility in a monastery. These results can be useful to design strategies to treat LTBI in the high TB burden area to achieve the goal of TB elimination.
Early triple negative breast cancers in a Singapore cohort exhibit high PIK3CA mutation rates associated with low PD-L1 expression
Authors: Yeong Joe , Goh Denise , Tan Tira J. , Tan Benedict , Sivaraj Huren , Koh Valerie , Lim Jeffrey Chun Tatt , Joseph Craig Ryan , Tay Timothy Kwang Yong , Ye Jiangfeng , Lau Mai Chan , Chan Jason Yongsheng , Iqbal Jabed , Ng Cedric Chuan Young , Teh Bin Tean , Dent Rebecca Alexandra , Tan Puay Hoon 
Published: August 2022

Abstract

Mutations in the PI3K pathway, particularly of PIK3CA, were reported to be intimately associated with triple negative breast cancer (TNBC) progression and development of treatment resistance. We profiled PIK3CA and other genes on 166 early-stage TNBC tumors from Singapore, for comparison to publicly available TNBC cohorts. These tumors were profiled transcriptionally using a Nanostring panel of immune genes and multiplex immunohistochemistry, then manually scored for PD-L1-positivity using two clinically relevant clones, SP142 and 22C3. We discovered a higher rate of PIK3CA mutations in our TNBC cohort as compared to non-Asian cohorts, along with TP53, BRCA1, PTPN11, and MAP3K1 alterations. PIK3CA mutations did not affect overall or recurrence-free survival, and when compared to PIK3CAWT tumors, there were no differences in immune infiltration. Using two clinically approved antibodies, PIK3CAmut tumors were associated with PD-L1 negativity. Analysis of co-mutation frequencies further revealed that PIK3CA mutations tended to be accompanied by MAP kinase pathway mutation. The mechanism and impact of PIK3CA alterations on the TNBC tumor immune microenvironment and PD-L1 positivity warrant further study.