bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preservice Subject Teachers’ Lack of Interest in Their Minor Subject: Is It a Problem?
Authors: Havia Johanna , Lutovac Sonja , Komulainen Tiina , Kaasila Raimo 
Published: April 2022

AbstractInterest in a particular subject is one central factor in preservice teachers’ decision to become subject teachers. Interest in a subject can also become visible in preservice teachers’ teaching and may consequently impact their students’ interest in the subject. However, the interest of preservice teachers in their major and minor subjects has been scarcely addressed. In Finland, secondary school teachers usually study and teach two or three subjects. Major subjects are often chosen based on interest; however, the reasons for choosing a minor subject can vary. In this study, we examined the narratives of three preservice subject teachers regarding their interest in their minor and major subjects. They all studied mathematics, physics, and chemistry and revealed a much weaker interest in the minor subjects compared to the major subjects. Interestingly, our findings also reveal that preservice teachers’ perceived strengths in a specific subject may not be as crucial to their interests, as has been suggested by prior research. We discuss the consequences of preservice teachers’ weaker interest in certain subjects and provide recommendations for teacher education.
Dominance or Tolerance? Causes and consequences of a period of increased intercommunity encounters among bonobos (Pan paniscus) at LuiKotale
Authors: Moscovice Liza R. , Hohmann Gottfried , Trumble Benjamin C. , Fruth Barbara , Jaeggi Adrian V. 
Published: April 2022

AbstractGroup-living primates exhibit variable reactions to intergroup encounters (or IGEs), reflecting species-specific strategies and individual motivations. In chimpanzees (Pan troglodytes), dominating in IGEs provides fitness benefits. Less is known about responses to IGEs in bonobos (Pan paniscus) despite their equal relevance for understanding the origins of human intergroup relations. We observed the Bompusa West (WBp) bonobo community at LuiKotale during a 2-month shift in ranging resulting in frequent IGEs with the smaller Bompusa East (EBp) community. We tested whether incursions provided ecological benefits, and whether responses to IGEs were consistent with inter-community dominance or tolerance. We measured fruit availability and collected activity scans from 26 mature WBp community members when in their core ranging area, during incursions into the EBp ranging area, and during IGEs. We collected data on sexual interactions and aggression with in-group and out-group members during 19 independent IGEs. During their shift in ranging, fruit availability was greater in the EBp ranging area, and WBp bonobos consumed more fruit during incursions than when in their core ranging area. Coalitionary intergroup aggression occurred during nine IGEs, and outcomes were consistent with imbalances in fighting power, in that larger WBp parties supplanted smaller EBp parties from the immediate area. However, communities reformed associations following 70% of coalitionary conflicts, and prolonged IGEs facilitated out-group sexual interactions and female transfers. The WBp community shift in ranging was likely motivated by ecological factors and responses to increased IGEs reflected a mixture of competitive and tolerant strategies.
Simulation-Based Investigation of a Probability of Detection (POD) Model Using Phased Array Ultrasonic Testing (PAUT) Technique
Authors: Lei Xiangyu , Wirdelius Håkan , Rosell Anders 
Published: April 2022

AbstractProbability of detection (POD) as a metric for quantifying the capability of inspection procedures in nondestructive evaluation (NDE), has been applied and evolved in industries since 1970s. Progress had been noted when certain statistical functions were brought up to model POD behavior, including log-normal model (also referred as Probit model). This model had been concluded to be the best fit and therefore has been widely used in many studies, while the involved assumptions and conditions were not carefully addressed and explored. To make flexible POD datasets available for specific inspection procedures and reduce the number of expensive experiments needed, model-assisted POD (MAPOD) is an alternative. This paper addresses a pure simulation-based POD procedure of an inspection scenario involving phased array ultrasonic testing (PAUT) on lack-of-fusion defects in additive manufactured (AM) components. The mathematical simulations are performed by an ultrasonic testing (UT) simulation software, simSUNDT, developed at Chalmers University of Technology in Sweden. Resulted inspection datasets with the proposed data processing steps are evaluated in terms of the assumptions and conditions of log-normal POD model, with the purpose of discussing the POD model validity under different circumstances. Simulation-based POD curves are finally compared with several discrete POD values at some defect sizes, calculated through massive computations from physics-model based metamodel. Comparisons and observations confirm satisfactory application of log-normal POD model despite some violations in model hypotheses.
The potential of three whole blood microRNAs to predict outcome and monitor treatment response in sarcoid-bearing equids
Authors: Hamza E. , Cosandey J. , Gerber V. , Koch C. , Unger L. 
Published: April 2022

AbstractMicroRNAs (miRNAs) have been proposed as biomarkers for equine sarcoid (ES) disease. In this study, the suitability of three whole blood miRNAs to diagnose ES and to predict and monitor the outcome of therapy was explored. Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), expression levels of eca-miR-127, eca-miR-379, and eca-miR-432 in whole blood of ES-affected equids before and at least one year after therapy were compared to those of unaffected control equids. Associations of age, sex, species, diagnosis, and therapy outcome with miRNA expression levels were examined using general linear models. In total, 48 ES-affected equids and 47 control equids were recruited. From the affected animals, 31 responded favorably to treatment, and 17 demonstrated a failure of therapy. None of the tested miRNAs were influenced by age. Male equids showed increased expression of eca-miR-127 compared to females and horses showed higher expression levels of eca-miR-379 and eca-miR-432 than donkeys. Eca-miR-127 was confirmed as a diagnostic discriminator between ES-affected and control equids. No difference in miRNA profiles before therapy was found when comparing ES-affected equids with success vs. failure of therapy. Eca-miR-379 and eca-miR-432 decreased over time in horses where therapy was successful, but not in those cases where it failed. Biological variables influence equine whole blood miRNA expression, which may complicate biomarker validation. While none of the tested miRNAs could predict the response to therapy in ES-affected equids and eca-miR-127 showed poor diagnostic accuracy for ES, eca-miR-379 and eca-miR-432 miRNAs might allow refinement of monitoring of success of ES therapy.
P2X7 receptor activation mediates superoxide dismutase 1 (SOD1) release from murine NSC-34 motor neurons
Authors: Bartlett Rachael , Ly Diane , Cashman Neil R. , Sluyter Ronald , Yerbury Justin J. 
Published: April 2022

AbstractMutant superoxide dismutase 1 (SOD1) can be constitutively released from motor neurons and transmitted to naïve motor neurons to promote the progression of amyotrophic lateral sclerosis (ALS). However, the biological impacts of this process and the precise mechanisms of SOD1 release remain to be fully resolved. Using biochemical and fluorescent techniques, this study aimed to determine if P2X7 receptor activation could induce mutant SOD1 release from motor neurons and whether this released SOD1 could be transmitted to motor neurons or microglia to mediate effects associated with neurodegeneration in ALS. Aggregated SOD1G93A, released from murine NSC-34 motor neurons transiently transfected with SOD1G93A, could be transmitted to naïve NSC-34 cells and murine EOC13 microglia to induce endoplasmic reticulum (ER) stress and tumour necrosis factor-alpha (TNFα) release, respectively. Immunoblotting revealed NSC-34 cells expressed P2X7. Extracellular ATP induced cation dye uptake into these cells, which was blocked by the P2X7 antagonist AZ10606120, demonstrating these cells express functional P2X7. Moreover, ATP induced the rapid release of aggregated SOD1G93A from NSC-34 cells transiently transfected with SOD1G93A, a process blocked by AZ10606120 and revealing a role for P2X7 in this process. ATP-induced SOD1G93A release coincided with membrane blebbing. Finally, aggregated SOD1G93A released via P2X7 activation could also be transmitted to NSC-34 and EOC13 cells to induce ER stress and TNFα release, respectively. Collectively, these results identify a novel role for P2X7 in the prion-like propagation of SOD1 in ALS and provide a possible explanation for the therapeutic benefits of P2X7 antagonism previously observed in ALS SOD1G93A mice.
Lead Nitrate Induces Inflammation and Apoptosis in Rat Lungs Through the Activation of NF-κB and AhR Signaling Pathways
Authors: Attafi Ibraheem M. , Bakheet Saleh A. , Ahmad Sheikh F. , Belali Osamah M. , Alanazi Fawaz E. , Aljarboa Suliman A. , AL-Alallah Ibrahim A. , Korashy Hesham M. 
Published: April 2022

AbstractLead (Pb) is one of the most frequent hazardous air contaminants, where the lungs are particularly vulnerable to its toxicity. However, the Pb distribution and its impact on lung inflammation/apoptosis and particularly the involvement of nuclear factor kappa B (NF-κB) and aryl hydrocarbon receptor (AhR) signaling pathways in Pb-induced lung toxicity have not yet been fully investigated. Adult male Wistar albino rats were exposed to Pb nitrate 25, 50, and 100 mg/kg b.w. orally for 3 days. The histopathological changes of several rat organs were analyzed using hematoxylin and eosin staining. The concentrations of Pb ion in different organ tissues were quantified using inductive coupled plasma mass spectrometry, while gas chromatography-mass spectrometry was used to identify organic compounds. The changes in the mRNA and protein expression levels of inflammatory and apoptotic genes in response to Pb exposure were quantified by using RT-PCR and Western blot analyses, respectively. Treatment of rats with Pb for three consecutive days significantly increased the accumulation of Pb in lung tissues causing severe interstitial inflammation. Pb treatment also increased the percentage of lung apoptotic cells and modulated apoptotic genes (Bc2, p53, and TGF-α), inflammatory markers (IL-4, IL-10, TNF-α), and oxidative stress biomarkers (iNOS, CYP1A1, EphX) in rat lung tissues. These effects were associated with a significant increase in organic compounds, such as 3-nitrotyrosine and myeloperoxidase, and some inorganic elements, such as selenium. Importantly, the Pb-induced lung inflammation and apoptosis were associated with a proportional increase in the expression of NF-κB and AhR mRNAs and proteins. These findings clearly show that Pb induces severe inflammation and apoptosis in rat lungs and suggest that NF-κB and AhR may play a role in Pb-induced lung toxicity.
Applying social life cycle assessment to evaluate the use phase of mobility services: a case study in Berlin
Authors: Gompf Katharina , Traverso Marzia , Hetterich Jörg 
Published: April 2022

Abstract
Purpose
The main purpose of this S-LCA case study is to analyse social impacts of the use phase of mobility services is a holistic way, taking into account positive as well as negative impacts. The use phase plays an important role for the assessment of services, which is why this study exclusively focuses on the use phase assessment. That way, this study aims to contribute to answer the question whether mobility services can improve quality of life in cities.

Methods
For the analysis, seven different mobility options were chosen in the city of Berlin, Germany, including free-floating car sharing, e-scooter sharing, S-Bahn, subway, tram, bus and the car in private ownership. For the analysis, five stakeholder groups that are outlined in the S-LCA Guidelines (UNEP 2020) were taken into account: Local Community, Consumer, Worker, Value Chain Actors and Society. For a detailed analysis of all relevant aspects, 37 indicators were analysed, out of which 23 are quantitative and 14 are qualitative. For data collection, several different data sources were used, including publicly available data e.g. from statistics as well as own data from interviews.

Results and discussion
For comparability, all results are displayed on a 5-point scale from − 2 to + 2, in line with the Handbook (Goedkoop et al. 2018) and the S-LCA Guidelines (UNEP 2020). For some indicators, the results of the case study are as expected, for example regarding impacts on air quality. For other indicators, however, the results are specific for the analysed mobility services in Berlin and therefore give new insights and reveal new aspects, as for example in the case of job creation for the local community. The main challenge of this S-LCA case study was data availability and data quality, which is why assumptions and simplifications had to be made, especially regarding space occupancy and the allocation of common infrastructure.

Conclusions
This S-LCA case study provides a holistic assessment of the use phase of mobility services, taking into account five stakeholder categories and their respective social impacts. The study illustrates specific results for the city of Berlin, showing positive as well as negative social impacts of mobility services and outlines a procedure for further studies. That way, this case study contributes to answer the overlying question whether mobility services can improve quality of life in cities.
Corrosion Effect on the Efficiency of High-Frequency Mechanical Impact Treatment in Enhancing Fatigue Strength of Welded Steel Structures
Authors: Al-Karawi Hassan 
Published: April 2022

AbstractFatigue and corrosion are two material degradation phenomena that occur in welded steel structures. High-frequency mechanical impact (HFMI) treatment is a post-weld treatment method that aims to increase the fatigue strength of welded details. This paper investigates the effect of steel corrosion on the efficiency of this method in enhancing fatigue resistance. More than 150 fatigue test results on corroded and HFMI-treated welded details are collected from several research articles and analyzed for both transverse welded attachment and butt-welded details. The efficiency of HFMI treatment decreases in corroded details as the corrosion level increases. However, HFMI treatment is found to have a high potential in prolonging the fatigue life, even in circumstances of an extremely corrosive environment.
Characteristics of tumour stroma in regional lymph node metastases in colorectal cancer patients: a theoretical framework for future diagnostic imaging with FAPI PET/CT
Authors: Polack Meaghan , Hagenaars Sophie C. , Couwenberg Alice , Kool Walter , Tollenaar Rob A. E. M. , Vogel Wouter V. , Snaebjornsson Petur , Mesker Wilma E. 
Published: April 2022

Abstract
Purpose
The recently developed fibroblast activation protein inhibitor (FAPI) tracer for PET/CT, binding tumour-stromal cancer-associated fibroblasts, is a promising tool for detection of positive lymph nodes. This study provides an overview of features, including sizes and tumour-stromal content, of lymph nodes and their respective lymph node metastases (LNM) in colorectal cancer (CRC), since literature lacks on whether LNMs contain sufficient stroma to potentially allow FAPI-based tumour detection.

Methods
Haematoxylin and eosin-stained tissue slides from 73 stage III colon cancer patients were included. Diameters and areas of all lymph nodes and their LNMs were assessed, the amount of stroma by measuring the stromal compartment area, the conventional and total tumour-stroma ratios (TSR-c and TSR-t, respectively), as well as correlations between these parameters. Also, subgroup analysis using a minimal diameter cut off of 5.0 mm was performed.

Results
In total, 126 lymph nodes were analysed. Although positive correlations were observed between node and LNM for diameter and area (r = 0.852, p < 0.001 and r = 0.960, p < 0.001, respectively), and also between the LNM stromal compartment area and nodal diameter (r = 0.612, p < 0.001), nodal area (r = 0.747, p < 0.001) and LNM area (r = 0.746, p < 0.001), novel insight was that nearly all (98%) LNMs contained stroma, with median TSR-c scores of 35% (IQR 20–60%) and TSR-t of 20% (IQR 10–30%). Moreover, a total of 32 (25%) positive lymph nodes had a diameter of < 5.0 mm.

Conclusion
In LNMs, stroma is abundantly present, independent of size, suggesting a role for FAPI PET/CT in improved lymph node detection in CRC.