bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ki-67 pulmonary Immunoreactivity in silver nanoparticles toxicity: size-rate dependent genotoxic impact
Authors: Ali Sanaa A. , Kadry Mai O. , Hammam Olfat , Hassan Sohair A. , Abdel-Megeed Rehab M. 
Published: August 2022

Abstract

BackgroundEngineered nanoparticles have been recently utilized in numerous domains particularly, silver nanoparticles (AgNPs). Nonetheless, the possible side effects resulting from AgNPs exposure are not fully clarified. The present study aimed to clarify the toxicity of AgNPs toxic effect on lung tissue. This study was extended to investigate the impact of Glycosmis pentaphylla (G. pentaphylla) and Casimiroa edulis (C. edulis) leaves extracts in addition to mucilage and protein; the purified compounds from C. edulis against AgNPs induced pulmonary toxicity. MethodsMale Swiss albino mice were administered AgNPs orally in two different particle sizes (20 nm and 100 nm) for one month and was further treated via the above mentioned natural product extracts in a dose of 500 mg/ kg for three weeks. Biochemical, molecular, immunohistochemistry, and histopathological investigations were further assessed. ResultsOur results declared an obvious alternation in oxidative stress biomarkers as well as mRNA gene expression of both survivin and matrix metalloproteinase (MMP-9) in addition to exploration of positive nuclei for ki-67. Data declared a significant upregulation of both glutathione s transferase and superoxide dismutase antioxidants up on treatment by C. edulis extract. Furthermore, a remarkable downregulation of MMP-9as well as survivin mRNA gene expression in all treated groups. Immunohistopathological examination investigated a significant improvement in the reactivity of ki-67 biomarker upon treatment. Histopathlgical examination confirmed the obtained results.ConclusionIn conclusion; these functional foods extracts could be considered as a promising candidate as therapeutic regimen against pulmonary toxicity induced via Ag-NPs due to their enrichment with different active constituents.
Supply Chain Impact from Production Capacity Constraints: A Computer-based Simulation Approach
Authors: Lockstrom Martin 
Published: August 2022

Abstract
The bullwhip is a well-known phenomenon in supply chain management. Whereas past research has focused on factors like supply rationing, batch-size, lead-time etc., mainly from an analytical perspective. This paper broadens the research by specifically examining the impact from production capacity constraints not only on demand (order size) but also on inventory levels and supply chain cost. The results confirm past research and brings new insights in the impact on component and compound bullwhip effect behavior across supply chain echelons by showing that production capacity acts as a damper on the bullwhip effect.
A randomized controlled trial of antibody response to 2020 cell-based inactivated and egg-based live attenuated influenza vaccines in individuals ages 4-21 years
Authors: Martin Judith , Williams Katherine , Li Zhu-Nan , Zhai Bo , Alcorn John , Nowalk Mary Patricia , Levine Min , Kim Sara , Flannery Brendan , Geffel Krissy Moehling , Merranko Amanda , Collins Mark , Susick Michael , Clarke Karen , Zimmerman Richard 
Published: August 2022

Abstract
Antibody responses to influenza vaccines may vary by many factors. Participants ages 4-21 were randomized to receive cultured inactivated influenza vaccine (ccIIV4; n =112) or live attenuated influenza vaccine (LAIV4; n=118). Hemagglutinin inhibition (HAI) reported in geometric mean titers (GMTs) and mean fold rise in titers (MFR) and IgG, IgA, and IgM antibodies reported in median fluorescence intensities (MFIs) were measured against cell-grown, egg-grown, and drifted A(H1N1) antigens, A(H1N1)pdm09, both vaccine B strains and the nucleoprotein (NP) from A(H3N2) on pre- and 28 days post vaccination. The HAI and immunoglobulin isotype response to ccIIV4 was greater than LAIV4. LAIV4 response was highest among the youngest participants. Prior LAIV4 vaccination was associated with a higher response to current season ccIIV4. Cross-reactive A/Delaware/55/2019(H1N1)pdm09 antibodies were present pre-vaccination and increased in response to ccIIV4, but not LAIV4. Immunoglobulin assays confirmed the findings of HAI titers to measure influenza vaccine immune response.Clinical Trials No.:﻿ NCT03982069
Grateful to Help You or Grateful to Help Me? An Investigation of the Effects of Expressing Gratitude to Benefit Oneself
Authors: Cregg David R. , Musarra Marley , Cheavens Jennifer S. 
Published: August 2022

Abstract
Gratitude interventions are often promoted as self-help exercises. However, expressing gratitude to benefit oneself may paradoxically undermine well-being, as this mindset shifts the focus away from the recipient of gratitude. Across two studies (N = 475), participants were randomly assigned to compose a gratitude letter using three rationales: self-focused (emphasizing benefits to the self), other-focused (emphasizing benefits to the recipient), and neutral (no rationale). The neutral group reported lower well-being than the two experimental groups (self-focused and other-focused) on a variety of outcomes. However, the experimental groups did not differ on main study outcomes, thus failing to support the hypothesis that self-focused reasons for expressing gratitude result in poorer well-being than other-focused reasons. However, text analyses revealed that the compositions of the letters differed between the self-focused and other-focused conditions. Individuals in the self-focused condition tended to write letters that were more self-referential, longer, less positive, and that used more insight/cognitive processing language, whereas individuals in the other-focused condition tended to write letters that were more other-referential, shorter, and more positive. Our results suggest that inclusion of a rationale enhances the effectiveness of a gratitude intervention, and the emphasis of the rationale can alter the way in which a gratitude letter is written. Implications for future research are discussed in light of these findings.
Implementation of Bayesian Hyperparameter Optimization for Predicting Student Dropout in Sub-Saharan Africa Secondary Schools
Authors: Mnyawami Yuda N. , Maziku Hellen , Mushi Joseph C. 
Published: August 2022

Abstract
Many secondary schools in Sub-Saharan countries face the problem of students dropping out of school due to various reasons which are difficult to diagnose directly. Various initiatives such as the big results now initiatives, free education for all, no child left behind, and secondary education development programme as well as machine learning prediction models used to reduce the severity of the problem in Sub-Saharan countries. The ongoing dropout problem, particularly in secondary schools, is ascribed to improper root cause identification and the absence of formal procedures that may be used to estimate the severity of the issue. Bayesian Optimization technique has been used to project the student dropout by optimizing the prediction results. The forecast accuracy of machine learning algorithms is hampered by default optimization techniques, making it difficult to pinpoint the real causes of student dropouts. The performance of the optimized model was evaluated by the average accuracy, precision, recall, f1, Roc curve, and AUC. Prediction accuracy results indicate that NB = 95%, LDA = 94%, SGD = 96%, DT = 97%, RF = 97%, LR = 96%, KNN = 97%, and AdaBoost = 97%. Features including student marks, age, school distance, parental education, number of children, and parental occupation significantly contribute to student dropouts. Results indicate the prediction accuracy of the proposed model outperforms the default optimization method of the machine learning algorithms. A well-optimized strategy draws a lot of attention to the findings related to student dropout rates in Sub-Saharan countries.
Identifying morphs of the Asian Hornet (Vespa velutina) and other pests of quarantine importance with geometric morphometrics
Authors: Smith-Pardo Allan Henry , Polly David 
Published: August 2022

Abstract

In this work assess the use of geometric morphometrics (GMM) as an identification tool for insects of quarantine importance that are difficult to identify based on traditional external morphology alone. We use the Asian hornet (Vespa velutina) as a case study because it is a highly variable species, with a broad natural distribution, and has been accidentally introduced in many regions of the world as a result of commerce. We used landmark data of the forewings of an extensive set of samples (previously considered “subspecies” of the Asian hornets) from across the native range of this species in southeastern Asia to assess the affinity of a dead specimen of the species that was intercepted dead on a mail parcel in Utah, USA in 2020. We find that GMM analysis of the forewing is able to statistically distinguish almost all color morphs from the native range with moderate-sized samples and that automated classification using discriminant function analysis is able to classify individuals with an overall accuracy of about 90%, ranging from 60–100% accurate depending on the morph. Discriminant function classification and continuous-trait maximum-likelihood clustering based on forewing shape both suggest that the Utah specimen belongs to thenigrithoraxcolor morph, which has a native range in southern China, but which has also been introduced to Europe and Korea. The GMM approach bears considerable promise for refining the identification and provenancing of intercepted specimens accurately and inexpensively and can be made even more powerful by increasing the coverage of the wing shape database.
Images of stylized faces enhance ERP features used for emotion detection
Authors: Chacón Luis Alberto Barradas , Brunner Clemens , Wriessnegger Selina C. 
Published: August 2022

Abstract
For their ease of accessibility and low cost, current Brain-Computer Interfaces (BCI) used to detect subjective emotional and affective states rely largely on electroencephalographic (EEG) signals. Numerous datasets are publicly available for any researcher to design models for affect detection from EEG. However, few designs focus on optimally exploiting the nature of the stimulus elicitation to improve accuracy. In this study, we found that artificially enhanced human faces with exaggerated visual features significantly improve some commonly used neural correlates of emotion as measured by event-related potentials (ERPs). These images elicit an enhanced N170 component, well known in facial recognition encoding. Our findings suggest that the study of emotion elicitation could exploit consistent stimuli transformations to study the characteristics of ERPs related to specific affective stimuli. Furthermore, this specific result might be useful in the context of affective BCI design, where a higher accuracy in affect detection from EEG can improve the experience of a user.
JAK inhibitor Tofacitinib alleviated acute hepatitis induced by lipopolysaccharide/D-galactosamine in mice
Authors: Zhang Xinyue , Lin Ling , Li Longjiang , Hu Kai , Shao Ruyue , Zhang Li , Tang Li , Zhu Min , Ma Yuhua , Yang Yongqiang 
Published: August 2022

Abstract
Background: To investigate the potential effects of JAK inhibitor Tofacitinib in mice with lipopolysaccharide/D-galactosamine (LPS/D-Gal)-induced acute hepatitis, including the production of inflammatory cytokines, the induction of hepatocytes apoptosis and the degree of liver injury were determined. Methods and Results: The plasma levels of ALT and AST and liver activities of caspase-3, caspase-8, caspase-9 were determined by colorimetric assay kits. The plasma levels of TNF-a and IL-6 were detected by ELISA kits. Hepatocellular apoptosis was observed by TUNEL assay. HE staining was used to observe the histopathological changes. The expression of cleaved caspase-3 was analyzed by western blot.The results indicated that treatment with Tofacitinib in LPS/D-Gal-induced acute liver injury decreased the levels of aminotransferases, attenuated the histological abnormalities in liver and decreased the plasma levels of TNF-a and IL-6. In addition, Tofacitinib suppressed the activation of caspase cascade, decreased the expression of cleaved caspase-3 and reduced the number of TUNEL-positive cells. Conclusion: Treatment with Tofacitinib alleviated LPS/D-Gal-induced acute hepatitis. JAK maybe become a promising target for the control of inflammation-based liver disorders.
Diagnostic Performance of Noninvasive Imaging Using Computed Tomography, Magnetic Resonance Imaging, and Positron Emission Tomography for the Detection of Ovarian Cancer: a Meta-analysis
Authors: Li Xiaoxiao , Wang Luqin , Guo Pengfei , Sun Qiangkun , Zhang Yating , Chen Cheng , Zhang Yulong 
Published: August 2022

Abstract

Objective The aim of this meta-analysis was to compare the diagnostic value of noninvasive imaging methods computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography (PET) in the detection of ovarian cancer (OC). Methods PubMed, Embase, and Ovid were comprehensively searched from the date of inception to 31st, March, 2022. Pooled sensitivity, specificity, positive likelihood ratio (+ LR), negative likelihood ratio (- LR), diagnostic odds ratio (DOR), and area under the curve (AUC) of summary receiver operating characteristic (SROC) with their respective 95% confidence intervals (CIs) were calculated. Results Sixty-one articles including 4284 patients met the inclusion criteria of this study. Pooled estimates of sensitivity, specificity, and AUC of SROC with respective 95% CIs of CT on patient level were 0.83 (0.73, 0.90), 0.69 (0.54, 0.81), and 0.84 (0.80, 0.87). The overall sensitivity, specificity, SROC value with respective 95% CIs of MRI were 0.95 (0.91, 0.97), 0.81 (0.76, 0.85), and 0.90 (0.87, 0.92) on patient level. Pooled estimates of sensitivity, specificity, SROC value of PET/CT on patient level were 0.92 (0.88, 0.94), 0.88 (0.83, 0.92), and 0.96 (0.94, 0.97). Conclusion Noninvasive imaging modalities including CT, MRI, PET (PET/CT, PET/MRI) yielded favorable diagnostic performance in the detection of OC. Hybrid implement of different tools (PET/CT or PET/MRI) is more accurate for identifying metastatic and recurrent OC.
Experience of pediatric nurses in nursing dying children- A Qualitative Study
Authors: bian weina , yang hongwei , cheng Junxiang , dong yue , xue ying , zhang qian , zheng qinghua , song rui 
Published: August 2022

Abstract
Purpose
To explore the challenges and effective coping strategies of pediatric nurses in caring for dying children.
Methods
A descriptive qualitative study was adopted.Data were collected using semi-structured interview with 10 nurses from the pediatrics, pediatric emergency department and neonatology department.
Results
Three themes were generated: stressors, stress consequences, coping strategies.Ten sub-themes were generalized: negative stress; helpless; question rescue behavior; fear of communication; lack of workforce for night rescue; compassion fatigue; burnout; changes in life attitudes; self-regulation; leadership approval and no accountability.
Conclusions
Through qualitative research, the challenges and effective coping strategies faced by nurses in caring for dying children were found, which provides information for nurses' career development and related policy formulation in China.
Clinical Relevance:
While there are many articles in China on hospice care, there is little research on the nurses’ experience of caring for dying children. In foreign countries, many studies have mentioned the adverse consequences of caring for dying children, and even led to post-traumatic stress disorder (PTSD). However,the domestic discussion of such problems is rare and there are no corresponding coping strategies.This study aims to explore the challenges and effective coping strategies of pediatric nurses in caring for dying children.
Vapor-responsive Shape Memory Material based on Carbon Nanotube Sponge dominated by Pressure-induced Structural transformation of Spidroin
Authors: Wang Yana , Chen Shuanglu , Zhang Jiapeng , Zhang Xiaohua , Li Yitan , Yang Zhaohui 
Published: August 2022

Abstract
Over decades, the confliction between excellent performances i.e. outstanding strength, elasticity, biocompatibility, and limited yields of the spider silks has become a severe challenge. Almost all the practical applications involve with the recombination of spidroin then the fabrication of artificial spider silks. Various attempts have been conducted to reveal both the components and the structures, while it remains insufficient to understand and copy the structural transition process of spidroin in practical applications. Herein by systematical experimental results, we feature that only a small hydrostatic pressure (several mega pascals) can induce the secondary structural transition of spidroin from α-helix to β-sheet, showing a tunable structural elasticity. Based on our understanding, we design and fabricated a vapor-responsive shape memory material by compositing both spidroin and carbon nanotube sponges (CNTS). The appearance of hydrostatic pressure can induce the secondary structural transition which terminates the elasticity of the CNTS and will recover under a simple solvent vapor annealing at ambient atmosphere, indicating a reversible “shape-memory” effect with the recovery efficiency close to 100 %. Our observations not only provide a promising shape-memory material with unique temperature-independent character, but reveal a crucial mechanism on the structural transformation of spidroin molecules under a confined environment, which will pave the way toward the design and fabrication of outstanding artificial spider silk-based products.
Efficacy and safety of first-line therapy for pulmonary nocardiosis; trimethoprim-sulfamethoxazole versus alternative antibiotics
Authors: Hamada Shohei , Inaba Megumi , Akaike Kimitaka , Okabayashi Hiroko , Masunaga Aiko , Tomita Yusuke , Okamoto Shinichiro , Tashiro Yasumasa , Hirata Naomi , Ichiyasu Hidenori , Sakagami Takuro 
Published: August 2022

Abstract
The recommended antibiotic for pulmonary nocardiosis (PN) is trimethoprim-sulfamethoxazole (TMP-SMZ). However, no comparative studies have assessed its efficacy and safety. We compared 180-day outcomes of first-line therapies for PN between fifty-two patients treated with TMP-SMZ (TS group [n = 18]) and with alternative antibiotics (non-TS group [n = 34]). Failure-free survival (FFS) was defined as the time from initiation to discontinuation of first-line therapy due to treatment failure including disease progression or death and adverse events. Overall survival (hazard ratio [HR] of TMP-SMZ, 2.96; p = 0.118) and the time to disease progression or death (HR of TMP-SMZ, 3.15; p = 0.136) did not differ significantly between the two groups. The incidence of adverse events was 61.1% (TS group) and 20.6% (non-TS group). The time to adverse events inducing treatment failure was significantly shorter (HR, 3.44; p = 0.021), and FFS was poorer in the TS group than in the non-TS group (HR, 3.33; p = 0.005). Multivariate Cox analysis revealed that TMP-SMZ administration was an independent predictor of treatment failure (HR, 3.16; p = 0.016). TMP-SMZ may not be an optimal antibiotic for first-line therapy for PN as it is inferior to alternative antibiotics in safety and associated with increased treatment failure.
Whole-genome sequencing of Pseudomonas koreensis isolated from diseased Tor tambroides
Authors: Kho Cindy Jia Yung , Chung Hung Hui , Lau Melinda Mei Lin , Chew Ivy Yee Yen , Gan Han Ming 
Published: August 2022

Abstract
BackgroundPseudomonas koreensis have been reported as a pathogen in freshwater fish. Unlike environmental P. koreensis from soil, which has been studied extensively for its role in promoting plant growth, pathogenic P. koreensis from fish has been assessed only to a very limited extent. This is the first draft genome of P. koreensis CM-01 isolated from the internal organs of diseased Tor tambroides.ResultsHerein, we report the morphological and biochemical characteristics, as well as whole genome sequences of a newly identified P. koreensis strain. We achieved a high-quality draft genome of P. koreensis CM-01 with a N50 value of 233,601 bp and 99.5% BUSCO completeness. The total size of P. koreensis CM-01 genome contains 6,171,880 bp and has a G+C content of 60.5%. Besides, the genome sequence includes 5538 protein-coding genes, 3 rRNA genes, 54 tRNAs, and no plasmids were found. Besides these, 39 interspersed repeat and 141 tandem repeat sequences, 6 prophages, 51 genomic islands, 94 insertion sequences, 4 clustered regularly interspaced short palindromic repeats, 5 antibiotic-resistant genes, and 150 virulence genes were predicted in the P. koreensis CM-01 genome. In addition, the assembled CM-01 genome was successfully annotated against the Cluster of Orthologous Groups of proteins database, Gene Ontology database, and Kyoto Encyclopedia of Genes and Genome pathway database. A comparative analysis of CM-01 with three representative strains of P. koreensis revealed that 92% of orthologous clusters were conserved among these four genomes, and only the CM-01 strain possesses unique elements related to pathogenicity and virulence.ConclusionsThis study provides fundamental phenotypic and genomic information for the newly identified P. koreensis strain. Further study will be necessary to fully understand any correlation between the bacteria and the occurrences of diseases in Tor tambroides.
A Pharmacodynamic Evaluation of the Protective Effects of Roxadustat Against Hypoxic Injury at High Altitude
Authors: Guo Qianwen , Li Xue , Li Wenbin , Wang Rong , Zhao Anpeng , Wang Zihan 
Published: August 2022

Abstract
Objectives: To explore the protective effects of roxadustat on hypoxia damage in the fast access to high altitude. Methods: BALB/C mice were randomly divided into control, roxadustat-7.8 mg/kg, roxadustat-15.6 mg/kg and roxadustat-31.2 mg/kg groups. The anti-hypoxic effectiveness of roxadustat in an optimal dose was evaluated by atmospheric pressure closed hypoxic experiment. Wistar rats were randomly divided into normal pressure, hypoxia model, acetazolamide and roxadustat groups to evaluate the protective effects against hypoxic damage. Animal blood was collected for arterial blood-gas analysis, cytokines detection, oxidative stress indicators, and their organs were harvested for pathological examination. Results: Compared with the control group, the survival time of mice were significantly prolonged in all groups. The time prolongation rate of the medium dose was 19.05%, which was the best dose. Compared with the hypoxia model group, the blood SatO2 and PaO2 in the roxadustat group were significantly increased; Erythrocyte content, hemoglobin content and hematocrit were significantly increased; Plasma levels of IL-6, TNF-α and IFN-γ were significantly decreased; MDA content in the myocardial, brain, lung and liver tissue were significantly decreased, SOD activity and GSH content in the tissue were significantly increased. The results of HE staining indicated that roxadustat could significantly improve the damage of heart, brain, lung, liver and kidney tissue after hypoxia in rats. Conlusions: Roxadustat can significantly prevent hypoxia-induced tissue damage, oxidative stress and inflammatory response indicating that roxadustat can obviously improve the adaptation to high-altitude exposure.