bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nighttime warming affected grain yield components, Cu accumulation and translocation of wheats grown in Cu-contaminated soil
Authors: Cheng Xianghan , Liu Feifei , Liu Xiaolei , Chen Xianni , Kou Taiji 
Published: June 2022

Abstract
Amis
This study aimed to uncover the changes in yield components, Cu accumulation and translocation of wheats in response to Cu pollution under nighttime warming.
Methods
Pot trials were adopted with two level of ambient temperature (no-warming (NT) and warming (WT)) and two level of soil Cu content (control check without Cu application (CK) and 100 mg/kg Cu application (Cu)). Canopy temperature, biomass, yield components, Cu content, accumulation and translocation factors of wheats, available Cu content and enzyme activities in soil were determined throughout the wheat growth period.
Results
Warming effect of passive nighttime warming system was prominent and average increment was 0.28°C. The highest yield was achieved in NT-Cu by many more spikes per pot and grains per spike, but the Cu content in its grains could present a potential risk to food security. Though Nighttime warming didn’t increase the total biomass and yield of wheats, soil urease and catalase activities, it decreased Cu accumulation of wheats grown in Cu-contaminated soil, especially in grains. Moreover, warming treatments increased the Cu content in the roots and glumes, reduced the content in grains. Among them, Cu content of grains in WT-Cu was the lowest and significantly lower than other applications.
Conclusions
Our findings reveal that nighttime warming has the potential to weaken the Cu risk of grains in wheats grown in Cu-contaminated soil.
Response of habitat quality to land use change in urban built-up area of karst mountainous city
Authors: Wei Wenfei , Wang Zhitai , Bao Yu , Chen Xintong , Sun Yuzhen , Zeng Mulin , Mo Yaguo 
Published: June 2022

Abstract
There are a large number of urban remnant mountains (URMs) in the built-up areas of karst mountainous cities, which are the main components of urban habitats and play a variety of irreplaceable ecosystem services functions such as maintaining local biodiversity. Based on InVEST model and geographically weighted regression (GWR), this study explored the relationship between land use/land cover (LULC) and habitat quality (HQ) evolution in a typical karst mountainous city. The results showed that: (1) the LULC change in the study area was intense from 2008 to 2018, with cultivated land and URMs transformed into construction land, and the urban landscape fragmentation was serious. (2) The overall level of HQ in the study area was low. The high value area was mainly distributed in the area where large urban green space (UGS) and URMs were located, while the low value area was mainly concentrated in the construction land gathering area. (3) GWR analysis showed that there was a significant correlation between LULC change and HQ change, natural environment determined the overall distribution of HQ, and human activities played a leading role in HQ change. (4) URMs was the main contributor of high level HQ, but its encroachment by construction land and the increase of surrounding building density make the quality of URMs habitat degraded obviously. This study is of great significance for urban biodiversity conservation and urban high-quality development.
A TTN-mutation-associated gene signature for prognostic prediction of hepatocellular carcinoma
Authors: Bi Yanqing , Huang Rong , Zhang ziying , Tian Zixuan , Liu min , Bao Han , Yan tao , Xia Yuan , Zhang nan , Zhang xingguang 
Published: June 2022

Abstract
Background: Mutation in titin (TTN), associated with a worsened prognosis, is among the most common genetic variants in human hepatocellular carcinoma. mRNA expression data and TTN mutation information from TCGA cohort were utilized to characterize a specific TTN-mutation-associated signature according to gene-expression differences between wild type (TTN-WT) and TTN-mutated (TTN-MUT) hepatocellular carcinoma (HCC) patients. In total, 189 differentially expressed genes (DEGs) potentially correlated with TTN mutation status were identified. Methods: Five genes (VAX1, MMP3, CXCL5, TKTL1 and KCNA3), identified by univariate Cox regression and Kaplan-Meier survival analyses, constituted an independent OS prognostic gene signature in HCC patients. Depending on this gene signature, patients were grouped into high- and low-risk subgroups and a significant enrichment associated with immunity is detected between the risk groups. In addition, the high-risk group was associated with an abundance of macrophages and neutrophils, while the infiltration indices of T follicular helper cells, natural killer cells, and type 2 helper T cells (Th2) were lower compared to low-risk group. In the multivariate Cox regression model, the five-gene signature remained a significant predictor independent of other conventional clinical risk factors used for survival prediction in HCC patients. MMP3 and CXCL5 expression were further validated in HCC cell lines.Results: We established a novel and unique TTN-mutation-related gene signature for survival outcome prediction for HCC patients. Conclusion: Our research foundings display a specific mechanism of TTN mutation and hold promise for the identification of HCC patients with poor prognosis.
Association of Non-carious Cervical Lesions With Oral Hygiene Aspects and Oclusal Force
Authors: Werneck Rafael Dario , Queiroz Daher Antonio , Rio Diana Leyva del , Altdorfer Balint Timothy Gabor , Turssi Cecilia Pedroso 
Published: June 2022

Abstract
One of the theories that contribute to explaining the evolution of non-carious cervical lesions (NCCLs) refers to fatigue wear, in which the concentration of stresses in the cervical region leads to the formation of microfractures in the dental tissues and progressive structure loss. However, due to a combination of factors that may cause wear, there is still uncertainty about the role of the occlusal factor as a risk factor that causes NCCLs. Objectives: The purpose of this case-control study was to investigate the association between the presence of NCCLs with occlusal force and other potential risk factors. Materials and Methods: Thirty-nine participants with NCCLs (cases) and 39 non-carrier patients (control), aged between 20 and 59 years old, were enrolled in the dental clinic of the Faculdade Integradas São Pedro (FAESA), located in Vitória, Espírito Santo State, Brazil. Information was collected through anamnesis, clinical examination, and a questionnaire addressing aspects related to tooth brushing, dentifrice, and mouthwash use. In clinical examination, patients were submitted to four measurements of occlusal force in the maxillary first premolars and maxillary first molars, using a strain gauge sensor of medium intensity, the Flexiforce (Tekscan, South Boston, MA, USA). The sensor was calibrated for the unit of measurement in Newtons (N). Results: Data was analyzed using a Student's t-test and multiple logistic regression, adopting the significance level of 5%. There was no statistically significant difference between the case and control groups regarding the bite force in the four measured regions. Logistic regression identified sex as a factor significantly associated with non-carious cervical lesions (p = 0.020). For the calculation of the odds ratio, the female sex presented a six-fold chance (OR = 6.082; CI = 1.332 - 27.765) higher than non-carious cervical lesions. Conclusions: It was concluded that females presented a higher risk factor for non-carious cervical lesions than men, whereas there was no association of occlusal strength, as well as aspects related to brushing and deleterious habits.Clinical Relevance: NCCLs are highly prevalent in daily clinical practices and can impact aesthetics and function, leading to hypersensitivity and impairment of oral-health-related quality of life. Gingival recession is an important clinical indicator for the presence of NCCLs.
Species Recognition of Bitten Leaves Based on Multi-Parameters with BP Neural Network
Authors: Rao Zhimin , Mao Jiandong , Zhao Hu , Zhou Chunyan , Gong Xin 
Published: June 2022

Abstract
There are many species of leaves in nature. The complete leaves are easy for identification, while bitten leaves hinder the recognition of leaf species. In order to accurately recognize the species of injured and damaged leaves, firstly, a recognition system GUI (Graphical User Interface) was designed, then a cameras of iPhone 12 Pro Max was used to collect complete and insect-bitten leaves pictures, obtain the color characteristics and shape characteristics of the leaves by image processing technology, and then combine neural network recognition algorithms to recognize the type of broken leaf with the insect bite. Results in this experiment show that this method can quickly and effectively recognize the species of bitten leaves, and provide an efficient and accurate recognition method for injured and damaged plant.
A super robust and efficient DNA storage architecture based on modulation encoding and decoding
Authors: Zan Xiangzhen , Xie Ranze , Yao Xiangyu , Xu Peng , Liu Wenbin 
Published: June 2022

Abstract
Thanks to its high density and long durability, synthetic DNA has been widely considered as a promising solution to the data explosion problem. However, due to the large amount of random base insertion-deletion-substitution (IDSs) errors from sequencing, reliable data recovery remains a critical challenge, which hinders its large-scale application. Here, we propose a modulation-based DNA storage architecture. Experiments on simulation and real datasets demonstrate that it has two distinct advantages. First, modulation encoding provides a simple way to ensure the encoded DNA sequences comply with biological sequence constraints (i.e., GC balanced and no homopolymers); Second, modulation decoding is highly efficient and extremely robust for the detection of insertions and deletions, which can correct up to ~ 40% errors. These two advantages pave the way for future high-throughput and low-cost techniques, and will kickstart the actualization of a viable, large-scale system for DNA data storage.
Study on machine learning of molar incisor hypomineralization in an endemic fluorosis region in central China
Authors: Zhang Yimeng , Wang Yu , Lu Yang , Zhang Zhaoxin , Wang Yuqi , Jia Jie 
Published: June 2022

Abstract
Objectives The aim of the present study was to develop machine learning model to predict the risk for MIH and identify associated factors in an endemic fluorosis region in central China.Materials and methods A cross-sectional study was conducted with selected regions of 1568 schoolchildren. The clinical examination included the investigation of MIH based on the European Academy of Paediatric Dentistry (EAPD) criteria. In this study, supervised machine learning (e.g., Logistic Regression) and correlation analysis (e.g., Pearson correlation analysis) were used for classification and prediction. Results Overall prevalence of MIH was 13.7%. The nomograph showed that non-dental fluorosis (DF) had great influence on the early occurrence of MIH that was decreased with the strengthen of DF severity. Then, we examined the association between MIH and DF and found DF had a protective correlation with MIH, and the effect of protection became more obvious with the increasing severity of DF. Furthermore, children whose enamel was defective were more prone to experience caries, meanwhile, dental caries was positively correlated with MIH (OR=1.843; 95% CI:1.260 -2.694). However, Exposure to poor quality shallow underground water, as well as gender and oral hygiene, did not increase the likelihood of developing MIH. Conclusions DF should be considered a protective factor within the multifactorial etiology of MIH.Clinical relevance In this study, the correlation between various factors, especially fluorosis, and MIH was explored, and a machine learning model was constructed to make it possible to predict the incidence of MIH. Our study may contribute to the early intervention and clinical planning towards the population with MIH.
CBFDR- Context-Based Fake News Detection and Reporting using Blockchain and Machine Learning
Authors: Mishra Himani , Jain Amita , Tayal Ankur 
Published: June 2022

Abstract
Cases of fake news are increasing at a high pace, especially through social media portals. Even though there are various fact checker websites and portals that distinguish news from real or fake news and ongoing research have been done to stop the propagation of fake news. , The major setback is timely detection, prevention and reporting of fake news. The proposed model CBFDR (Context-Based Fake News Detection and Reporting) first analyzes the domain of the given news through Amazon web services (AWS) Comprehend, secondly detects the fake news through RNN-LSTM (Recurrent neural network – Long short term memory & XG-Boost algorithm (extreme gradient) and thirdly includes a set of domain-experts who analyze the set of fake news as well as report in Block-chain (using smart contract and proposed algorithm Proof of Evidence (PoE)) to stop the propagation of fake news at an early stage. The given paper has shown the highest success rate of 98% using RNN-LSTM and 93% using the XG-Boost algorithm for fake news detection. This paper covers detection, prevention and reporting in one application with an average 60-70% faster and more accurate than the existing models.
The effects of taurine supplementation on obesity and browning of white adipose tissue in high-fat diet-fed mice.
Authors: Bagci Gokhan , Okten Hatice 
Published: June 2022

Abstract
Background: In recent years, a new type of adipose tissue (beige adipose tissue) has been mentioned, unlike white adipose tissue (WAT) and brown adipose tissue (BAT). Beige cells are capable of thermogenesis like BAT. In response to various agents, beige cells can develop within WAT through a process called “browning”. Therefore, the prevention of obesity and related diseases by providing WAT browning with new potential agents has been extensively studied in recent years. Taurine has many physiological functions in the body and has beneficial effects on obesity and related metabolic disorders. For this reason, we aimed to investigate whether taurine supplementation has effects on browning of WAT and attenuating obesity.Methods: Thirty-two male C57BL/6 mice were used for the study. Mice were divided into 4 groups as control, control + taurine, high fat diet (HFD) and HFD + taurine, and fed for 20 weeks. Taurine was given in drinking water (5%). Epididymal WAT samples were obtained from mice and RNA was extracted from these tissues. Expression levels of FLCN, mTOR, TFE3, PGC-1α, PGC1-1β, AMPK, S6K and UCP1 genes were measured by real-time PCR.Results: Taurine supplementation reduced HFD-induced obesity. No UCP1 expression was detected in any of the groups studied. Any of the gene expressions were not significantly different between HFD and HFD + taurine groups. Reduced PGC-1α and PGC-1β expressions were observed in both HFD and HFD + taurine groups.Conclusions: Taurine reduced the obesity in HFD fed mice, but had no effect on browning of epididymal WAT in this study.
Length Weight Relationship of five marine fishes associated with trawler bycatch with record of maximum total length from Eastern Indian Ocean
Authors: Karuppasamy K. , B Sureandiran , Jawahar P. , Tamilselvan R. , Venkataramani V.K. 
Published: June 2022

Abstract
Length-Weight Relationship (LWR) parameters were analyzed for five marine fish species associated with commercial trawler as bycatch from the Tamil Nadu coast, Eastern Indian Ocean: Rhynchostracion nasus, Scolopsis xenochrous, Stethojulis albovittata, Triacanthus nieuhofii and Engyprosopon grandisquama belonging to the five families viz., Ostraciidae, Nemipteridae, Labridae, Triacanthidae and Bothidae. Fishes were sampled monthly between August 2019 to March 2020 from the landings of trawlers operated along the coast of Tuticorin, Chinnamuttom and Colachel, Tamil Nadu coast, with a cod-end mesh size of 35 mm operated at the depth of 20-80 m. Values of the parameter b ranged from 2.734 for Stethojulies albovittata to 3.3300 for Rhynchostracion nasus and a values varied from 0.004115 Rhynchostracion nasus, 0.00809 Scoliopsis xenochrous respectively. All LWRs were significant with r2 values ranged from 0.80441 to 0.9912. In addition, this study provides the information on LWRs and also recorded a new maximum total length for Rhynchostracion nasus, Triacanthus nieuhofii and Engyprosopon grandisquama.
Anti-cancer effect of hUC-MSC-derived exosome-mediated delivery of PMO-miR-146b-5p in colorectal cancer
Authors: Yu siming , Liao ran , Bai lu , Guo madi , Zhang yu , Zhang yumin , Yang qi , Song yushuai , Li zhiwei , Meng qingwei , Wang shubin , Huang xiaoyi 
Published: June 2022

Abstract
BackgroundAntisense oligonucleotide (ASO) is a novel therapeutic platform for targeted cancer therapy. Colorectal cancer (CRC) is the third leading cause of cancer mortality and the prognosis of CRC is poor when hepatic metastasis occurs. We have demonstrated that miR-146b-5p plays an important role in CRC progression. Knocking down miR-146b-5p by an ASO is expected to slow down the progression. However, a safe and effective strategy for delivery of an ASO to its targeted RNA remains as a major hurdle in translational advances.ResultsHuman umbilical cord mesenchymal cell (hUC-MSC)-derived exosomes were used as vehicles to deliver an anti-miR-146b-5p ASO (PMO-146b) to CRC cells. PMO-146b was assembled onto the surface of exosomes(e) through a covalently conjugated anchor peptide CP05(P) that recognized an exosomal surface marker, CD63, forming a complex named ePPMO-146b. The conjugate (ePPMO-146b) was taken up by SW620 cells and effectively inhibited cellular proliferation and epithelial-to-mesenchymal transition. It also exerted antitumor efficacy in a xenograft mouse model of colon cancer by systematic administration, where PPMO-146b was enriched in tumor tissue. Of particular importance was selective distribution of ePPMO-146b in liver compared to native exosomes through in vivo imaging, indicating its therapeutic potential to inhibit hepatic metastasis of CRC.ConclusionOur study highlights the potential of hUC-MSC-derived exosomes anchored with PPMO-146b as a novel safe and effective approach for ASO delivery.
Extracellular epimorphin impairs expression and processing of profilaggrin in HaCaT keratinocytes
Authors: Hori Haruna , Kotani Ayaka , Abe Junya , Matsuguchi Shuji , Hirai Yohei 
Published: June 2022

Abstract
The expression and processing of filaggrin, a filament-associated protein in the skin epidermis, is closely associated with keratinocyte cornification. The large precursor profilaggrin (Pro-FLG) is initially detected at the granular layer in keratohyalin granules, subsequently processed into ten to twelve filaggrin monomers (mFLGs) for keratin assembly, and ultimately degraded into smaller peptides that behave as natural moisturizing factor (NMF) at the outermost epidermis. We previously reported that a t-SNARE protein epimorphin (EPM) often translocates across the membrane upon external stimuli and severely perturbs epidermal terminal differentiation. Using HaCaT keratinocytes with inducible expression and recombinant EPM and FLG, we investigated the effect of extracellular EPM on the expression profile of filaggrin. In response to ectopic stimulation with extracellular EPM, Pro-FLG expression decreased with elimination of keratohyalin granules in the cells, with filaggrin mRNA remained constant and profilaggrin processing was not accelerated. Additionally, using a recombinant form of mFLG engineered for intracellular localization, we found that extracellular EPM hindered proteolytic cleavage of mFLG for production of NMF. Taken together, extracellularly extruded EPM, an epidermal cornification blocker, not only decreases Pro-FLG expression but also reduces the production of NMF in HaCaT keratinocytes.
Peripheral and posterior pole retinal changes in highly myopic Chaoshan Chinese children and adolescents Retinal changes in Chinese children and adolescents
Authors: Zhang Wenli , Chen Shirong , Shi Tingkun 
Published: June 2022

Abstract
Purpose: To determine the prevalence and factors associated with peripheral and posterior pole retinal changes in highly myopic Chinese children and adolescents.Methods: A prospective, consecutive case series and cross-sectional study was designed. One-hundred-and-twenty subjects with high myopia were recruited and underwent cycloplegic refraction, dilated fundus examination, and optical coherence tomography. Statistical analysis was performed to evaluate the factors associated with the development of peripheral and posterior pole retinal changes.Results: The mean spherical equivalent refraction of the subjects was - 8.74 ± 2.86 D, and the mean age of the subjects was 11.45 ± 3.02 years. The most common peripheral retinal changes were snowflake retinal degeneration (27.5%), white without pressure (27.5%), snail-track degeneration (15.00%), and lattice degeneration (15.00%). The most common posterior changes were optic nerve crescent (78.3%) and posterior staphyloma (11.7%). Subjects with peripheral retinal changes had significantly older age and higher myopia compared with subjects without peripheral retinal changes (12.08 ± 3.08 vs. 10.71±2.79 years, -9.26 ± 3.25 vs. -8.13 ± 2.19 D, respectively, both p < 0.05). The presence of optic nerve crescent was associated with thinner choroidal thickness and a higher degree of refractive error (183.35 ± 62.1 µm vs. 219.69 ± 66.82 µm, -9.00 ± 3.08 vs. -7.81 ± 1.55 D, respectively, both p < 0.05).Conclusion: A substantial proportion of the subjects were found to have peripheral and posterior retinal changes. There is an increased risk of retinal changes in eyes with a higher degree of myopia, longer axial length, and thinner choroidal thickness in individuals aged 7–16 years.
EEUCWFL: Energy Efficient Unequal Clustering
With Multi-Input Multi-Output Fuzzy Logic
Authors: PANDEY SHIVENDRA KUMAR , SINGH BUDDHA 
Published: June 2022

Abstract
The Wireless Sensor Network (WSN) consists a group of sensors and a Base Station (BS) or sink. Sensors gather data from the sensing area and send these data to BS, either directly or through multi-hop data transmission. There are several methods of efficient data transmission. Clustering is one of them. WSN uses the many different algorithms for making the cluster to transmit the data from the physical environment to the BS. Clustering

makes the WSN Network efficient and improves the network lifetime. Other data transmission methods like direct transmission consumes very much energy so the life span of the sensor nodes becomes low in comparison to the clustering algorithm. The disadvantage of multi-hop data transmission of clustering algorithm is that a cluster head closer to sink has to forward the data of its own cluster as well as the data of near by clusters. So the node near base station dies sooner. This problem is known as hot-spot problem. The solution of hot-spot problem is unequal clustering. Nodes near base station should be smaller in size to balance the remaining energy of the nodes. In this paper, we have proposed a clustering algorithm (EEUCWFL) which uses multi-input multi-output fuzzy logic to select an optimal cluster head with unequal cluster size. We compared our proposed algorithm with three best known algorithms LEACH, EEUC and CHEF. The Results show that our proposed algorithm optimize energy consumption and improves the network lifetime.
50 Years with Nordic Hydrology/Hydrology Research
Authors: Rosbjerg Dan 
Published: June 2022

Abstract
The history of Nordic Hydrology/Hydrology Research is described from its initiation in 1970 to its current state in 2021. This includes dramatic changes leading first to an ownership transformation to Nordic Association for Hydrology (NAH) in 1976, and much later, in 2004, to a joint ownership between NAH, British Hydrological Society and IWA Publishing. The development in publication policy during time is covered as well.