bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of accountability on local officials’ behavior in the pandemic prevention and control in China based on utility maximization
Authors: Zeng Shian , Yi Chengdong 
Published: September 2022

Abstract
Background
The COVID-19 pandemic is a public health crisis and an inspection of national governance systems and crisis response capabilities of countries globally. China has adopted a tough accountability system for officials and has succeeded in containing the spread of the pandemic. This study aimed to assess the impact of accountability on local officials’ behavior in the pandemic prevention and control based on the official promotion tournament theory and utility maximization analysis framework.

Methods
The panel data of 237 Chinese cities were extracted with local officials’ characteristics, confirmed cases, Baidu migration index, Baidu search index according to city names, and data were excluded with local officials’ relocation or sub-provincial cities between January 1, 2020 and May 5, 2020. Promotion gain and accountability cost were constructed by adopting promotion speed indicator, and the research hypotheses were assumed based on the utility maximization. It was the first time to apply the interaction model to empirically investigate the relationship between the promotion speed of local officials and the COVID-19 confirmed cases.

Results
Our study showed that the promotion speed of provincial governors and mayors significantly affected the number of confirmed cases (β = − 11.615, P < 0.01). There was a significant interaction between the promotion speeds of provincial governors and mayors (β = − 2594.1, P < 0.01), indicating that they had a coordinated effect on the pandemic control. Additionally, mayors with different promotion speeds made a significant difference in controlling the imported cases and those who promoted faster better controlled the imported cases (β = − 0.841, P < 0.01). Mayors with full-time postgraduate education, titles, and majors in science and engineering had a better effect on controlling the number of confirmed cases.

Conclusions
Our study provides evidence that the official accountability system has played an important role in containing the pandemic, which suggests that local officials motivated by the accountability system would respond to the pandemic actively for higher utility. Furthermore, provincial governors and mayors have played a coordinated effect in pandemic control. The above evidences reveal that implementing the official accountability system could improve the government’s emergency management capability and the efficiency of pandemic control. Therefore, adopting a strict accountability system could be effective in pandemic containment globally, especially in centralized countries.
Mediastinal aberrant goiter characterized by high radiodensity on non-enhanced computed tomography: a case report
Authors: Suzuki Mikito , Horio Hirotoshi , Nakamura Azusa , Shimizu Reiko , Shima Toshiyuki , Harada Masahiko , Hishima Tsunekazu 
Published: September 2022

Abstract
Background
A mediastinal aberrant goiter is extremely rare, characterized by independence from the thyroid gland. This tumor most commonly develops in the anterior mediastinum and should be differentiated from a thymoma. Moreover, as mediastinal aberrant goiters frequently have a blood supply arising from the thoracic great vessels, preoperative diagnosis and evaluation of the feeding vessel are vital for safe surgery. Herein, we report a rare case of mediastinal aberrant goiter characterized by high radiodensity on non-enhanced computed tomography (CT).

Case presentation
A 77-year-old woman underwent non-enhanced CT, which showed a 2.2-cm, well-circumscribed, homogeneous anterior mediastinal nodule with high radiodensity. The CT attenuation value of the nodule was as high as 91.9 Hounsfield units. A thymoma or mediastinal goiter was suspected owing to the tumor location and the lack of a cystic component. We performed anterior mediastinal tumor resection using video-assisted thoracoscopic surgery. Pathologically, variably sized follicles, indicative of thyroid tissue, were observed with hemorrhage and hemosiderin deposition. Moreover, papillary projections of small follicles (Sanderson’s polsters) were scattered without atypia of the follicular epithelium, indicative of an adenomatous goiter. The diagnosis of a mediastinal aberrant goiter was supported by the absence of an anatomical connection with the thyroid gland. The postoperative course was uneventful, and the patient was discharged on postoperative day 5.

Conclusion
The thyroid gland has a high radiodensity on non-enhanced CT, which correlates with iodine concentration. This radiological feature may be useful for the preoperative differentiation of mediastinal aberrant goiters containing thyroid tissue.
Targeting apoptosis pathways in infections
Authors: Naderer Thomas , Fulcher Maria Cecilia 
Published: September 2022

Abstract
The programmed cell death pathway of apoptosis is essential for mammalian development and immunity as it eliminates unwanted and dangerous cells. As part of the cellular immune response, apoptosis removes the replicative niche of intracellular pathogens and enables the resolution of infections. To subvert apoptosis, pathogens have evolved a diverse range of mechanisms. In some circumstances, however, pathogens express effector molecules that induce apoptotic cell death. In this review, we focus on selected host-pathogen interactions that affect apoptotic pathways. We discuss how pathogens control the fate of host cells and how this determines the outcome of infections. Finally, small molecule inhibitors that activate apoptosis in cancer cells can also induce apoptotic cell death of infected cells. This suggests that targeting host death factors to kill infected cells is a potential therapeutic option to treat infectious diseases.
Ligand activation of nerve growth factor receptor TrkA protects monocytes from apoptosis
Authors: la Sala Andrea , Corinti Silvia , Federici Monica , Saragovi H Uri , Girolomoni Giampiero 
Published: September 2022

Abstract
Nerve growth factor (NGF) receptors are expressed in different cell types outside the nervous system, and increasing evidence indicates that NGF can act as a regulatory molecule during inflammatory and immune responses. In this study, we show that triggering of the high-affinity NGF receptor TrkA with agonists protects monocytes from apoptosis induced by gliotoxin or UVB radiation. TrkA stimulation up-regulates the expression of the anti-apoptotic Bcl-2 family members, Bcl-2, Bcl-XL, and Bfl-1. On the other hand, TrkA stimulation does not change the expression of MHC, CD80, CD86, CD40, and CD54 molecules, nor the antigen-presenting function of monocytes. In addition, during in vitro monocyte to dendritic cell differentiation TrkA expression is progressively lost, suggesting that NGF selectively affects monocyte but not dendritic cell survival.
IL-4 production by human polymorphonuclear neutrophils
Authors: Brandt Eric , Woerly Gaëtane , Younes Amena Ben , Loiseau Sylvie , Capron Monique 
Published: September 2022

AbstractPolymorphonuclear neutrophils (PMN) are phagocytic cells, able to secrete a large range of cytokines, including inflammatory cytokines, chemokines, as well as the Th1 cytokines interferon-γ (IFN-γ) and interleukin (IL)-12. Although PMN do not seem to express IL-10 and IL-13, no information exists on the ability of PMN to produce IL-4. Therefore intracellular flow cytometry was performed in the presence or absence of Brefeldin A. Similarly to eosinophils, freshly isolated neutrophils from normal donors contained low amounts of IL-4, which significantly increased upon culture with Brefeldin A (P &lt; 0001). Immunostaining performed on cytospin preparations of normal granulocytes confirmed the presence of intracellular IL-4. Using a highly sensitive ELISA, the levels of IL-4 secreted by cultured PMN and peripheral blood mononuclear cells (PBMC) were compared. PBMC secrete up to 60 times more IL-4 as PMN but, in the presence of calcium ionophore, only PMN showed a slight but significant increase in IL-4 secretion (P &lt; 0.05). In conclusion, we report here the presence within human PMN of intracellular IL-4, which can at least partly be released under calcium ionophore stimulation. The relevance of this production of IL-4 by human PMN is discussed.
Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies
Authors: Shakhov Alexander N , Lyakhov Ilya G , Tumanov Alexei V , Rubtsov Anatoly V , Drutskaya Ludmila N , Marino Michael W , Nedospasov Sergei A 
Published: September 2022

AbstractMice with combined lymphotoxin-α (LTα) and tumor necrosis factor (TNF) deficiencies show defects in the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut-associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen, we applied a gene profiling approach, including subtractive cloning and gene array hybridizations, to mice with combined TNF/LT deficiency. The differentially expressed genes identified by these techniques was then evaluated by Northern blot analysis for splenic expression in knockout mice with single LTα or single TNF deficiency. Most of the genes detected in this analysis are directly or indirectly associated with disrupted LT and not TNF signaling.
Expression on human eosinophils of CD148: a membrane tyrosine phosphatase. Implications in the effector function of eosinophils
Authors: del Pozo Victoria , Pirotto Fiorella , Cárdaba Blanca , Cortegano Isabel , Gallardo Soledad , Rojo Marta , Arrieta Ignacio , Aceituno Esther , Palomino Pilar , Gaya Antoni , Lahoz Carlos 
Published: September 2022

AbstractThe role of protein tyrosine phosphatases (PTP) is crucial in regulating the phosphorylation status of cells. CD148 is a recently described membrane-type PTP. In this study, we have demonstrated that this molecule is expressed on human eosinophils and eosinophilic cell line EoL-3. Interestingly, our data also showed that this molecule acts as a transduction molecule on these cells. Thus, the crosslinking of CD148 was able to induce the degranulation and the induction of superoxide anion generation. By using specific inhibitor and by western blotting, we have shown that tyrosine kinase activation is involved in this transduction pathway. In addition, we have shown the presence of a serine/threonin kinase activity associated with CD148. In conclusion, the activation capacity of CD148 on eosinophils suggests a potential role of this molecule on inflammatory diseases, such as allergic and parasitic diseases, associated with eosinophilia.
Preferential sites for stationary adhesion of neutrophils to cytokine-stimulated HUVEC under flow conditions
Authors: Gopalan Priya K , Burns Alan R , Simon Scott I , Sparks Scott , McIntire Larry V , Smith C Wayne 
Published: September 2022

Abstract
Neutrophils form CD18-dependent adhesions to endothelial cells at sites of inflammation. This phenomenon was investigated under conditions of flow in vitro using isolated human neutrophils and monolayers of HUVEC. The efficiency of conversion of neutrophil rolling to stable adhesion in this model was &gt;95%. Neither anti-CD11a nor anti-CD11b antibodies significantly altered the extent of this conversion, but a combination of both antibodies inhibited the arrest of rolling neutrophils by &gt;95%. The efficiency of transendothelial migration of arrested neutrophils was &gt;90%, and the site of transmigration was typically &lt;6 μm from the site of stationary adhesion. Approximately 70% of transmigrating neutrophils migrated at tricellular corners between three adjacent endothelial cells. A model of neutrophils randomly distributed on endothelium predicted a significantly greater migration distance to these preferred sites of transmigration, but a model of neutrophils adhering to endothelial borders is consistent with observed distances. It appears that stable adhesions form very near tricellular corners.
T cell lysis of murine renal cancer: multiple signaling pathways for cell death via Fas
Authors: Sayers Thomas J , Brooks Alan D , Seki Naoko , Smyth Mark J , Yagita Hideo , Blazar Bruce R , Malyguine Anatoli M 
Published: September 2022

AbstractActivated T cells lyse the murine renal cancer Renca. We have examined the mechanism of tumor cell lysis with the use of T cells derived from C57BL/6, BALB/c, B6.gld, and B6.Pfp-/- mice. C57BL/6 and BALB/c T cells can lyse Renca cells through the use of both granule- and Fas ligand (FasL)-mediated pathways. However, B6.gld T cells predominantly use granule-mediated killing, whereas B6.Pfp-/- T cells use FasL. The lysis of Renca by Pfp-/- T cells is only partially inhibited by the caspase inhibitor ZVAD-FMK, suggesting that caspase-independent signaling is also important for Renca cell lysis. When the reactive oxygen scavenger butylated hydroxyanisole was used alone or in combination with ZVAD-FMK a substantial reduction of Renca lysis was observed. Therefore, the caspase-independent generation of reactive oxygen intermediates in Renca after Fas triggering contributes to the lysis of these cells.
Oxidative stress as a necessary factor in room temperature-induced apoptosis of HL-60 cells
Authors: Shimura Mari , Osawa Yutaka , Yuo Akira , Hatake Kiyohiko , Takaku Fumimaro , Ishizaka Yukihito 
Published: September 2022

Abstract
HL-60 cells undergo apoptosis when placed at room temperature (RT) [Shimura et al. (1997) FEBS Lett. 417, 379–384]. We report that superoxide anion radical, one of the reactive oxygen species (ROS), was produced after RT treatment. Affinity blot analysis with a biotinylated YVAD-CHO detected the generation of processed peptides with molecular masses of 15–25 kDa. Activation of such an ICE-like protease was completely abolished by N-acetylcysteine and exogenously expressed Bcl-2, known as antioxidants. We concluded that oxidative stress was a critical factor in the signal cascade of the apoptosis. Western blot analysis and experiments using tetrapeptide inhibitors suggested that caspases-1, -3, -4, -6, and -9 did not have an essential role in the apoptotic cascade. It is interesting that cyclosporin A (CsA) blocked RT-induced apoptosis with an inhibition of cytochrome c release from mitochondria. CsA, however, generated a significant amount of ROS with considerable reduction of mitochondrial membrane potential, implying that oxidative stress was one necessary factor for RT-induced apoptosis. It is also likely that mitochondrial membrane potential and the release of apoptotic factors from cytoplasm are differently regulated. Taken together with the reports that some Burkitt lymphoma cells showed apoptosis when exposed at low temperature followed by rewarming, and that hepatocytes or liver endothelial cells are susceptible to cold-induced apoptosis through the ROS function, we propose that studying the mechanism of RT-induced apoptosis of HL-60 cells may provide a therapeutic strategy for pathological conditions involving ROS, such as neurodegenerative diseases and ischemia.
Chemokine signaling and HIV-1 fusion mediated by macrophage CXCR4: implications for target cell tropism
Authors: Collman Ronald G , Yi Yanjie , Liu Qing-Hua , Freedman Bruce D 
Published: September 2022

Abstract
To better understand CXCR4 function on macrophages and the relationship between coreceptor use and macrophage tropism among diverse HIV-1 isolates, we analyzed macrophage pathways involved in Env-mediated fusion, productive HIV-1 infection, and chemokine-elicited signaling. We found that both CXCR4 and CCR5 transduced intracellular signals in monocyte-derived macrophages, activating K+ and Cl− ion channels and elevating intracellular calcium in response to their chemokine ligands stromal cell-derived factor-1α and macrophage inflammatory protein-1β, respectively. The prototype T-tropic X4 strain IIIB infected macrophages poorly, and this was associated with failure of the IIIB Env to fuse efficiently with target macrophages despite functional CXCR4. In contrast, several primary X4 isolates mediated efficient CXCR4-dependent fusion and productive macrophage infection. Several R5X4 strains could fuse with and infect macrophages through both CCR5 and CXCR4. Thus, macrophages express functional CXCR4 and CCR5 but primary and prototype X4 isolates differ in their ability to utilize macrophage CXCR4. Isolates classified as X4 based on coreceptor use may be phenotypically either T-tropic or dual-tropic and, conversely, phenotypically dual-tropic isolates may be either R5X4 or X4 based on coreceptor use.
Co-receptor usage was more predictive than NSI/SI phenotype for HIV replication in macrophages: is NSI/SI phenotyping sufficient?
Authors: Lathey Janet L , Brambilla Donald , Goodenow Maureen M , Nokta Mostafa , Rasheed Suraiya , Siwak Edward B , Bremer James W , Huang Diana D , Yi Yanjie , Reichelderfer Patricia S , Collman Ronald G 
Published: September 2022

Abstract
A monocyte-derived macrophage (MDM) culture assay was used to define the replication kinetics of HIV isolates. Ten-day-old MDMs were infected with HIV. Supernatants were collected and assayed for HIV p24 on days 3, 7, 10, and 14 post-infection (PI). In this assay, SF162 (macrophage tropic, NSI) produced increasing amounts of HIV p24 antigen with increasing time in culture. BRU (nonmacrophage tropic, SI) infection resulted in low levels of HIV p24 antigen with no increase in production during the culture period. A panel of 12 clinical isolates was evaluated. All isolates produced detectable levels of HIV p24 antigen in MDMs. However, the NSI viruses had significantly higher log10 HIV p24 antigen values at all times PI (P &lt; 0.01). Co-receptor usage was determined for all 12 isolates (8 NSI and 4 SI). All SI isolates used CXCR4 for entry; two used CXCR4 only, one used CXCR4, CCR5, and CCR3, and one was a mixture of two isolates using CXCR4 and CCR5. None of the NSI viruses used CXCR4 for entry. All used CCR5 as their predominant co-receptor. Of the eight NSI isolates, three used CCR5 only, two used CCR5 and CCR2b, one used CCR5 and CCR3, and one used CCR5, CCR3, and CCR2b. Log10 HIV p24 antigen production on day 14 PI for viruses that used CCR5+CCR3 (3.79 + 1.40) was greater than for viruses that used CCR5+CCR2b (3.22 + 1.55) or CCR5 (3.32 + 1.49), and all were greater than those that used CXCR4 only (1.69 + 0.28), regardless of SI phenotype (P &lt; 0.05). Thus, in these primary isolates, macrophage tropism and replication kinetics were closely linked to CCR5 utilization, whereas SI capacity was closely linked to CXCR4 utilization. Furthermore, viruses, which could use CCR5 and CCR3 for entry, had a replication advantage in macrophages, regardless of SI phenotype.
Monocyte:astrocyte interactions regulate MCP-1 expression in both cell types
Authors: Andjelkovic Anuska V , Kerkovich Danielle , Pachter Joel S 
Published: September 2022

Abstract
As astrocytes are a source of monocyte chemoattractant protein-1 (MCP-1) and lie in close apposition to brain microvessels, interactions between astrocytes and infiltrating monocytes might regulate production of this chemokine. To investigate this possibility, a monocyte:astrocyte co-culture model was utilized to assess the respective roles of these two cell types in regulating MCP-1 production. Results indicate that, while neither monocytes nor astrocytes alone produce detectable levels of MCP-1, co-culture of these two cell types results in time-dependent production of this chemokine. Such production requires de novo protein synthesis and is dependent on physical contact between monocytes and astrocytes, involving engagement of the cell-adhesion molecules ICAM-1 and VCAM-1. Additionally, interleukin 1-beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) are soluble mediators of this response. These findings imply that monocyte extravasation into the CNS may be critically regulated at the blood-brain barrier by specialized monocyte:astrocyte interactions.
Recognition of CHO cells by inhibitory and activating Ly-49 receptors
Authors: Mason Llewellyn H 
Published: September 2022

Abstract
Upon ligand recognition, members of the murine Ly-49 receptor family can transmit inhibitory or activating signals that regulate NK cell function. Ly-49A, G, and D have been shown to recognize the murine class I molecule H-2Dd as a potential ligand. Recent studies also have demonstrated also that Ly-49D+ NK cells can lyse CHO cells, although the ligand responsible for this recognition was not identified. Because allorecognition by NK cells may be important in bone-marrow transplantation and because of the overlapping class I recognition by these receptors, recognition of CHO cells by Ly-49G and A was investigated. Our data suggest that Ly-49G and probably A transmit inhibitory signals in response to CHO cells. Receptor inhibition was assessed by examining NK lytic function, IFN-γ secretion, and DAP12 phosphorylation in response to CHO cells by sorted subsets of Ly-49D vs. G B6 NK cells. Our results suggest that CHO cells may express a common ligand(s) that is capable of engaging Ly-49D, G, and possibly A in C576BL/6 NK cells. In addition to our findings that Ly-49 inhibitory receptors also recognize CHO cells, activating receptors other than Ly-49D are present in B6 mice that can lyse CHO cells.
Src kinase-mediated signaling in leukocytes
Authors: Korade-Mirnics Željka , Corey Seth J 
Published: September 2022

Abstract
A concert of antigens, antibodies, cytokines, adhesion molecules, lipid factors, and their different receptors mediate leukocyte development and inflammatory responses. Regardless of the stimulus and receptor type, members of the Src family of protein tyrosine kinases (PTKs) play a critical role in initiating the numerous intracellular signaling pathways. Recruited and activated by the receptor, these Src PTKs amplify and diversify the signal. Multiple pathways arise, which affect cell migration, adhesion, phagocytosis, cell cycle, and cell survival. Essential nonredundant properties of Src PTKs have been identified through the use of gene targeting in mice or in the somatic cell line DT40. Because of their role in mediating leukocyte proliferation and activation, Src PTKs serve as excellent drug targets. Inhibitors of Src family members and dependent pathways may be useful in the treatment of human diseases similar to drugs known to inhibit other signal transduction pathways.
Structural requirements for intracellular processing and sorting of bactericidal/permeability-increasing protein (BPI): comparison with lipopolysaccharide-binding protein
Authors: Bülow Elinor , Gullberg Urban , Olsson Inge 
Published: September 2022

AbstractThe bactericidal/permeability-increasing protein (BPI), which is stored in the azurophil granules of neutrophils, and the circulating lipopolysaccharide-binding protein (LBP) share the same structure. Both bind lipopolysaccharide of gram-negative bacteria through their amino-terminal domains. The carboxy-terminal domain of BPI promotes bacterial attachment to phagocytes, whereas the corresponding domain of LBP delivers lipopolysaccharide to monocytes/macrophages. Our aim was to investigate the role of the amino- and carboxy-terminal domains of BPI and LBP for sorting and storage in myeloid cells after transfection of cDNA to two rodent hematopoietic cell lines. Full-length BPI and LBP were both targeted for storage in these cells. Deletion of the carboxy-terminal half of BPI resulted in storage followed by degradation while the reciprocal deletion of the amino-terminal half led to retention in the endoplasmic reticulum for proteasomal degradation. Chimeras between halves of BPI and LBP were also targeted for storage, but those containing carboxy-terminal BPI had the highest stability, again indicating a role for the carboxy-terminal domain of BPI in protection against degradation. Therefore, we propose a critical stability function for the hydrophobic carboxy-terminal domain of BPI during intracellular sorting for storage while the amino-terminal domain may confer targeting for storage.