bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of serum levels of interleukins 1‐beta, 10 and 12 in patients with acne vulgaris
Authors: Mohamed Marwa , Shehata Hanan Abdel Aaty , Fahmy Nahed Fathallah , Saleh Ramadan 
Published: September 2022

AbstractIntroductionOne of the most prevalent skin disorders is acne vulgaris. It is a distressing problem among young adults and adolescents. The pathophysiology of AV is significantly influenced by certain immune response cells. Acne's localized tissue inflammatory reactions are brought on by the bacteria Cutibacterium acnes, which also causes the release of several inflammatory mediators.Aim of the WorkTo measure the serum levels of IL‐1 and IL‐12, two pro‐inflammatory cytokines, and IL‐10, an anti‐inflammatory cytokine, in AV patients and link those levels with the severity of the condition.ResultsThis study included 19 males and 23 females with AV(n = 42), with mean age of 21.79 ± 3.5. The control group included 19 males and 23 female (n = 42) with mean age of 22.05 ± 3.3 (p = 0.729). Serum levels of IL‐1β was significantly higher in patients with AV as than controls (p < 0.001). Similarly, the serum levels of IL‐12 were significantly higher in AV cases than controls (p < 0.001). On the contrary, the median IL‐10 was significantly lower in AV cases than controls (p = 0.015). The correlation between serum levels of IL‐1β and IL‐12 in AV patients and disease severity was insignificant (r = −0.04, p = 0.404) and (r = −0.19, p = 0.118, respectively). (r = −0.19, p = 0.118) On the contrary, there was a significant negative correlation between serum levels of IL‐10 in AV patients and disease severity (r = −0.43, p = 0.003).ConclusionOur results indicate significant alterations in the monocyte‐derived cytokine (MDCs) profile in patients with AV, IL‐1 and IL‐12 serum levels rose while IL‐10 levels fell, reflecting an increase in pro‐inflammatory cytokines and a decrease in anti‐inflammatory cytokines. These highlight the important role played by monocytes in the pathogenesis of AV.
Spurious laboratory results associated with immunoglobulin M gammopathy in a dog with multiple myeloma
Authors: Loane Samantha C. , Castillo Daniel A. , Peschard Anne‐Lorraine D. M. , Hall Harriet R. , Kortum Andre J. 
Published: September 2022

AbstractAn 11 year old female‐neutered Labrador presented for facial swelling. Clinicopathological abnormalities included hyperglobulinemia, azotemia, hypercalcemia, nonregenerative anemia, thrombocytopenia, and spurious hypoglycemia. Normoglycemia was subsequently confirmed using a cage‐side analyzer (AlphaTRAK, Zoetis, UK). Serum and urine protein electrophoresis documented monoclonal (immunoglobulin M) gammopathy with Bence‐Jones proteinuria. Computed tomography imaging revealed a monostotic osteolytic bone‐lesion, and bone marrow cytology and histopathology documented plasmacytosis with multiple myeloma oncogene 1 / interferon regulatory factor 4 positivity, consistent with multiple myeloma. Infectious disease testing initially indicated seropositivity forLeishmania,Borrelia, andAnaplasmaspp.; however,LeishmaniaPCR (splenic and bone marrow aspirates), and paired serological titers forBorreliaandAnaplasmawere negative. Consequently, initial serological results were considered to be false positive because of paraproteinemia‐associated assay interference. Chemotherapy (prednisolone and melphalan combination therapy) was initiated, but the dog was euthanased 30 days later because of the development of pericardial effusion. This is a report of spurious serological (and other laboratory) results occurring secondary to monoclonal gammopathy in a dog.
Novel genome sequence of Chinese cavefish (Triplophysa rosa) reveals pervasive relaxation of natural selection in cavefish genomes
Authors: Zhao Qingyuan , Shao Feng , Li Yanping , Yi Soojin V. , Peng Zuogang 
Published: September 2022

AbstractAll cavefishes, living exclusively in caves across the globe, exhibit similar phenotypic traits, including the characteristic loss of eyes. To understand whether such phenotypic convergence shares similar genomic bases, here we investigated genome‐wide evolutionary signatures of cavefish phenotypes by comparing whole‐genome sequences of three pairs of cavefishes and their surface fish relatives. Notably, we newly sequenced and generated a whole‐genome assembly of the Chinese cavefish Triplophysa rosa. Our comparative analyses revealed several shared features of cavefish genome evolution. Cavefishes had lower mutation rates than their surface fish relatives. In contrast, the ratio of nonsynonymous to synonymous substitutions (ω) was significantly elevated in cavefishes compared to in surface fishes, consistent with the relaxation of purifying selection. In addition, cavefish genomes had an increased mutational load, including mutations that alter protein hydrophobicity profiles, which were considered harmful. Interestingly, however, we found no overlap in positively selected genes among different cavefish lineages, indicating that the phenotypic convergence in cavefishes was not caused by positive selection of the same sets of genes. Analyses of previously identified candidate genes associated with cave phenotypes supported this conclusion. Genes belonging to the lipid metabolism functional ontology were under relaxed purifying selection in all cavefish genomes, which may be associated with the nutrient‐poor habitat of cavefishes. Our work reveals previously uncharacterized patterns of cavefish genome evolution and provides comparative insights into the evolution of cave‐associated phenotypic traits.
A methodology for scheduling within‐day roadway work zones using deep neural networks and active learning
Authors: Saneii Mostafa , Kazemeini Ali , Seilabi Sania Esmaeilzadeh , Miralinaghi Mohammad , Labi Samuel 
Published: September 2022

AbstractCity infrastructure agencies routinely implement road projects that address various elements of urban infrastructure. The majority of these projects are short‐term in nature (e.g., utility repair), as they are completed in a few hours within 8:00 a.m. to 5:00 p.m. of a workday. The implementation of these projects during working hours, in spite of the inconvenience imposed on road users, helps the agency avoid extra labor costs associated with nonregular working hours. Careful scheduling of these projects can prevent unduly increased travel delays (road users’ interest) while keeping project costs low (the agency's interest). This study presents a bi‐level framework for scheduling short‐term urban road projects that analyzes the implicit tradeoffs between the two stakeholders’ interests. The upper‐level model establishes the optimal schedule considering the project characteristics, such as cost and duration. The lower‐level model captures the dynamic user equilibrium conditions that yield the road users’ path and departure time choices. The bi‐level model is a mixed‐integer program with nonlinear constraints. Recognizing the relatively low efficiency of traditional solution methods, this paper proposes a deep‐neural‐network‐ensemble‐assisted active learning (DN2EA2L) algorithm and adopts a fixed‐point algorithm for solving the bi‐level model. The numerical experiment uses the Sioux Falls network to demonstrate the efficiency of the DN2EA2L, compared to conventional metaheuristic methods. It is shown that travel time increases due to the project implementation during the peak hours can outweigh the agency's saving in wage costs. Further, it is shown that a significant reduction in the road users’ value of time enables the agency to schedule more projects during regular working hours.
Math anxiety as an independent psychological construct among social‐emotional attitudes: An exploratory factor analysis
Authors: Cheng Dazhi , Ren Bingqian , Yu Xiaodan , Wang Haitao , Chen Qian , Zhou Xinlin 
Published: September 2022

AbstractMath anxiety is a widespread problem for children and adolescents worldwide. However, the psychological trait of math anxiety is poorly understood. The present study aimed to examine the psychological construct of math anxiety among social‐emotional attitudes. A total of 28,726 students, including 17,378 fourth graders and 11,348 eighth graders, were selected from the Qingdao Basic Education Quality Assessment database using multi‐stage cluster random sampling. There were 10 questionnaires assessing social‐emotional attitudes in the database. Pearson's correlation analyses were performed to examine the intercorrelations between social‐emotional attitudes and mathematical performance. Exploratory factor analysis (EFA) was conducted to examine the psychological structure of these 10 social‐emotional attitudes. After controlling for other social‐emotional attitudes, math anxiety had a weak but significant correlation to mathematical performance in most subtests across grades. Among three‐factor, four‐factor, and five‐factor EFA models, math anxiety was an independent factor that was separate from other social‐emotional attitudes across six parallel subtests in grades four and eight. Math anxiety is a stable and independent psychological construct that is separate from other social‐emotional attitudes. It suggests that math anxiety should be considered as a distinct anxiety disorder specific to mathematical learning in subsequent versions of the Diagnostic and Statistical Manual of Mental Disorders.
Water retention and metabolic changes improve desiccation tolerance in Barbacenia graminifolia (Velloziaceae)
Authors: Vieira Evandro Alves , Silva Kleber Resende , Rossi Mônica Lanzoni , Martinelli Adriana Pinheiro , Gaspar Marilia , Braga Marcia Regina 
Published: September 2022

AbstractBarbacenia graminifolia is a Velloziaceae species endemic to the campos rupestres in Serra do Cipó, Minas Gerais state (Brazil). This biome is characterised by high irradiance and limited water conditions. Unlike other resurrection plants, B. graminifolia can maintain a high hydric status (>80%) after 28 days of water suppression before desiccation. We investigated the physiological and metabolic mechanisms associated with structural changes that allow B. graminifolia to maintain hydration under a prolonged water deficit and to recover after desiccation. After 30 days of water deficit, desiccated plants exhibited chlorophyll degradation, a 178.4% and 193.7% increase in total carotenoids and MDA, respectively, and twice the CAT and APX activity compared to hydrated plants. The metabolite profile showed increased amino acids, carbohydrates, saturated fatty acids and benzoic acids during dehydration, while trichloroacetic acid cycle acids were higher in hydrated and rehydrated plants. Anatomical and ultrastructural data corroborated the physiological and metabolic changes and revealed the presence of mucilaginous cells with high water retention capacity. Our data indicated that combined strategies of assimilatory metabolism shutdown, accumulation of compatible solutes and antioxidant compounds, increase in hydrophilic molecules, changes in the composition of membrane lipids and remodelling of cell organelles conditioned the efficiency of B. graminifolia in delaying water loss, tolerating further desiccation and quickly recovering after rehydration. These attributes evidence that this species is well adapted to cope with adverse environmental conditions, mainly directing the metabolism to an efficient antioxidant response and improving its capacity to retain water during the dry season.
The contribution of mother‐father‐child interactions to children's emotion narratives
Authors: Adler Shira Yuval , Oppenheim David 
Published: September 2022

AbstractParents' sensitivity during interactions with their children has been associated with children's emotion narratives elicited using story completion tasks, but almost all of this research focused on mothers and was based on a dyadic, parent‐child focus. The goal of the present study was to expand this research by studying triadic, mother‐father‐child interactions and their associations with children's narratives. Seventy‐one families with their 4.5‐year‐old children were observed in the Lausanne Trilogue Play (LTP) procedure to assess Family Cooperation, and children were administered the MacArthur Story‐Stem Battery (MSSB) to assess Coherence, Parental Representations, and Engagement with the task. Regression analyses indicated that controlling for children's Narrative Competence and Effortful Control, children who were part of more cooperative family interactions produced narratives that were more coherent, and they were more engaged during the task. No associations were found with children's representations of parents. Directions for future studies as well as clinical implications are discussed.
Hepatitis E virus is effectively inactivated by methylene blue plus light treatment
Authors: Gravemann Ute , Engelmann Michael , Kinast Volker , Burkard Thomas , Behrendt Patrick , Schulze Torsten J. , Todt Daniel , Steinmann Eike 
Published: September 2022

AbstractBackground and objectivesPhotodynamic treatment with methylene blue (MB) and visible light is a well‐established pathogen inactivation system for human plasma. This technique is routinely used in different countries. MB/light treatment was shown to inactivate several transfusion‐transmittable viruses, but its efficiency for the inactivation of the quasi‐enveloped hepatitis E virus (HEV) has not yet been investigated.Materials and methodsPlasma units were spiked with cell culture‐derived HEV and treated with the THERAFLEX MB‐Plasma system using various light doses (30, 60, 90, and 120 J/cm2). HEV titers in pre‐ and post‐treatment samples were determined by virus titration and a large‐volume plating assay to improve the detection limit of the virus assay.ResultsTHERAFLEX MB‐Plasma efficiently inactivated HEV in human plasma. Even the lowest light dose of 30 J/cm2 inactivated HEV down to the limit of detection, with a mean log reduction factor of greater than 2.4 for the total process.ConclusionOur study demonstrates that the THERAFLEX MB‐Plasma system effectively inactivates HEV in human plasma.
Echocardiographic indicators of fluid responsiveness in hospitalized dogs with compromised hemodynamics and tissue hypoperfusion
Authors: Donati Pablo A. , Villalta Cesar , Lisa Tarragona , Fravega Rodrigo , Cordero Ignacio Sandez , Tunesi Marcela , Guevara Juan Manuel , Otero Pablo E. 
Published: September 2022

AbstractObjectiveTo evaluate the accuracy of selected echocardiographic variables used to predict fluid responsiveness in hospitalized dogs with compromised hemodynamics and tissue hypoperfusion.DesignDiagnostic test study in a prospective cohort of hospitalized dogs.SettingVeterinary referral clinics.AnimalsForty‐four hospitalized dogs with compromised hemodynamics and tissue hypoperfusion were utilized in this study.InterventionsEchocardiographic examination before and after fluid replacement with 30 ml/kg of lactated Ringer's solution.Measurements and Main ResultsPre‐fluid replacement measurements of velocity of transmitral E wave (E‐peak), the left ventricular end‐diastolic internal diameter normalized to body weight (LVIDdN), and the left ventricular end‐systolic internal diameter normalized to body weight (LVIDsN) were significantly lower in fluid‐responsive patients compared with nonresponders (P < 0.001). The area under the receiver operating characteristic curve (AUROC) with its 95% confidence interval (CI) for each significant predictor was as follows: E‐peak 0.907 (0.776–1.000, P < 0.001) and LVIDdN 0.919 (0.801–1.000, P < 0.001). The predictive capacity of LVIDsN was not significantly better than chance (AUROC, 0.753; 95% CI, 0.472–1.000, P = 0.078). A significant negative linear correlation was observed between the percentage of increase in velocity–time integral after expansion and the echocardiographic variables LVIDdN (rs = –0.452, P = 0.023) and E‐peak (rs = –0.396, P = 0.008) pre‐fluid replacement. The intraobserver and interobserver variability was very low (<5 %) for all measurements.ConclusionsIn this study using critically ill dogs with compromised hemodynamics and tissue hypoperfusion, pre‐fluid replacement measurements of LVIDdN and E‐peak adequately predict fluid responsiveness. Because a small number of fluid nonresponders were involved in the present study (11.4%), further studies that include larger numbers of fluid‐nonresponsive animals are required.
Retrospective study of 20 cats surgically treated for insulinoma
Authors: Veytsman Stan , Amsellem Pierre , Husbands Brian D. , Rendahl Aaron K. , Bergman Philip J. , Chanoit Guillaume , Hickey Colleen , Cash Kristy R. , Maritato Karl C. , Collivignarelli Francesco , Kirpensteijn Jolle 
Published: September 2022

AbstractObjectiveTo report the clinical signs, histopathology results, and prognostic factors for outcomes following excision for feline insulinoma (INS).Study designRetrospective study.Sample populationTwenty client‐owned cats.MethodsMedical records from 2006 to 2020 were reviewed by Veterinary Society of Surgical Oncology members for cats with hypoglycemia resulting from INS, with surgical excision and follow up. Clinical signs and histopathology results were summarized. Factors potentially related to disease‐free interval (DFI), disease‐related death (DRD), and overall survival time (OST) were analyzed with a Cox proportional hazards regression analysis.ResultsAll cats were hypoglycemic on presentation with neurologic signs in 18 out of 20 and inappropriate insulin levels in 12/13. Excision of insulinomas resulted in immediate euglycemia or hyperglycemia in 18 cats. Eighteen cats survived to hospital discharge. The median time to death or last postoperative follow up was 664 days (range: 2‐1205 days). Prognostic factors included age at presentation (for DFI); time to postoperative euglycemia (for DRD); preoperative and postoperative serum blood glucose concentrations; metastasis at the time of surgery (DFI and DRD), and histopathologic tumor invasion (for OST). The median OST for all cats was 863 days. The 1‐, 2‐ and 3‐year survival rates were 75%, 51%, and 10%, respectively.ConclusionExcision of insulinoma resulted in euglycemia or hyperglycemia in most cats. Negative prognostic factors included young age, low serum glucose concentrations, metastasis at time of surgery, tumor invasion, and shorter time to euglycemia.Clinical significanceSurgical excision resulted in survival times comparable to those of canine INS.
A compositional analysis of native and decellularized porcine nasal septum cartilage
Authors: Tluczynski Katharina , Breiter Roman 
Published: September 2022

AbstractObjectivesDecellularization of porcine septum cartilage is necessary for its application as xenogenic replacement material. The aim of this study was to investigate spatial differences of structure and composition in the whole native and decellularized porcine nasal septum. Subsequently, the results shall be compared with studies of human nasal septum.MethodsTen porcine nasal septa were divided into six regions from caudal to cephalic and four regions from dorsal to ventral to create a grid of 24 approximately equal segments. All segments of five septal cartilages were decellularized separately by a wet chemical multistep procedure. The segments were analyzed to determine quantitative amounts of total collagen, chondrocytes, and sulfated glycosaminoglycans (sGAG).ResultsThe distribution of cell number showed no significant differences between the individual regions. For the distribution of collagen and sGAG, no significant differences could be identified from caudal to cephalic, both in native and decellularized tissue. From dorsal to ventral, native and decellularized nasal septum showed significant differences between individual regions. In native septum, linear regression analysis indicated a decreasing collagen and an increasing sGAG content from dorsal to ventral. After decellularization, an increasing collagen and a decreasing sGAG content was detected.ConclusionThe results of this study showed slightly but significant differences in the distribution of collagen and sGAG from dorsal to ventral. From caudal to cephalic, no differences could be observed. Compared to human, nasal septum differences in cell, collagen, and sGAG content were detected. Despite this, human and porcine nasal septum showed similar distributions and a consistently inverse linearity of collagen and sGAG content. Nevertheless, the midcaudal and midcephalic regions showed the highest porosity and a high stability and thus offer the best conditions for the revitalization of porcine tissue by human cells.
Phylogeny of European Pyrochroa (Coleoptera, Pyrochroidae) reveals cryptic taxa and different glacial histories
Authors: Molfini Marco , Mancini Emiliano , Bologna Marco A. 
Published: September 2022

AbstractOnly three saproxylic species of Pyrochroinae (Coleoptera: Pyrochroidae) are distributed in Europe, two of which belonging to Pyrochroa: P. coccinea and P. serraticornis. However, P. serraticornis is polytypic, for the presence of the endemic subspecies P. s. kiesenwetteri in southern Italy. Using both molecular and morphological data, we explored the phylogeny of the European Pyrochroa species. A multilocus (COI, CAD, 28S) phylogenetic analysis helped highlight different evolutionary histories for the two examined species. First, P. coccinea, distributed throughout Europe, showed a high differentiation among Italian and European populations. Furthermore, three different taxonomic entities were identified within P. serraticornis, among which the cryptic species Pyrochroa bifoveata sp. n. from central Europe is described and illustrated. A comprehensive identification key to the European Pyrochroinae is also provided. Our results also suggested an historical survival of P. coccinea and P. s. kiesenwetteri in glacial refugia in Italy, and a subsequent post‐glacial spread of the former species throughout the Peninsula. In contrast, the current distribution of P. s. serraticornis likely originated from a post‐glacial colonization of western European relict populations, while the survival of P. bifoveata plausibly occurred in more eastern glacial refugia (e.g. Carpathian or Balkan regions). Similarly, the European populations of P. coccinea could have originated from relict populations in glacial refugia out from the Italian Peninsula. More comprehensive data on the taxonomy, ecology and biogeography of Pyrochroa are needed to learn more about these species and to help preserve the European saproxylic fauna.