bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnetic Field Annihilation in a Magnetotail Electron Diffusion Region With Electron‐Scale Magnetic Island
Authors: Hasegawa H. , Denton R. E. , Nakamura T. K. M. , Genestreti K. J. , Phan T. D. , Nakamura R. , Hwang K.‐J. , Ahmadi N. , Shi Q. Q. , Hesse M. , Burch J. L. , Webster J. M. , Torbert R. B. , Giles B. L. , Gershman D. J. , Russell C. T. , Strangeway R. J. , Wei H. Y. , Lindqvist P.‐A. , Khotyaintsev Y. V. , Ergun R. E. , Saito Y. 
Published: June 2022

AbstractWe present observations in Earth's magnetotail by the Magnetospheric Multiscale spacecraft that are consistent with magnetic field annihilation, rather than magnetic topology change, causing fast magnetic‐to‐electron energy conversion in an electron‐scale current sheet. Multi‐spacecraft analysis for the magnetic field reconstruction shows that an electron‐scale magnetic island was embedded in the observed electron diffusion region (EDR), suggesting an elongated shape of the EDR. Evidence for the annihilation was revealed in the form of the island growing at a rate much lower than expected for the standard X‐type geometry of the EDR, which indicates that magnetic flux injected into the EDR was not ejected from the X‐point or accumulated in the island, but was dissipated in the EDR. This energy conversion process is in contrast to that in the standard EDR of a reconnecting current sheet where the energy of antiparallel magnetic fields is mostly converted to electron bulk‐flow energy. Fully kinetic simulation also demonstrates that an elongated EDR is subject to the formation of electron‐scale magnetic islands in which fast but transient annihilation can occur. Consistent with the observations and simulation, theoretical analysis shows that fast magnetic diffusion can occur in an elongated EDR in the presence of nongyrotropic electron effects. We suggest that the annihilation in elongated EDRs may contribute to the dissipation of magnetic energy in a turbulent collisionless plasma.
The Roles of Heat and Gas in a Mushy Magma Chamber
Authors: Liao Yang 
Published: June 2022

AbstractCrustal magmatic systems likely consist of magmatic reservoirs dominated by crystal mush. Recent studies suggest that the physical processes occurring in crystal mush could alter the response of magmatic reservoirs during volcanic unrest. Here, we present a magma chamber deformation model that incorporates two new aspects in crystal mush: heat and exsolved gas. The model is based on earlier studies by Liao et al. (2021, https://doi.org/10.1029/2020JB019395) with additional processes including thermal‐mechanical coupling, dependence of material properties on gas content, and temperature evolution following an injection of hotter magma. The post‐injection time‐dependent evolution of the system can be grouped into three periods, which are dominated by poroelastic diffusion (short term), viscoelastic relaxation (mid term), and thermal equilibration (long term). All three time‐regimes are strongly affected by gas distribution, which alters the relative compressibility of the crystal‐rich and crystal‐poor regions in the chamber. The contribution of thermal evolution emerges during the mid‐term evolution. The time‐dependent evolution of the system highlights the intrinsic ability of a gas‐bearing mushy magma chamber to generate non‐monotonic time series of stresses, deformation, and magma transport.
Non‐Gaussian Distributions of TOA SW Flux as Observed by MISR and CERES
Authors: Lee Jae N. , Wu Dong L. 
Published: June 2022

AbstractThe Top of Atmosphere (TOA) shortwave (SW) flux, converted from Terra Multi‐angle Imaging SpectroRadiometer (MISR) narrow band albedos, is compared with that measured from Clouds and the Earth's Radiant Energy System (CERES). We describe the probability density function (PDF) of the monthly TOA SW flux and how the statistical third moment, skewness, can impact the quantification of the flux. The PDF of the SW flux is not normally distributed but positively skewed. In both sets of observations, the near‐global (80 S–80 N) median value of the SW flux is ∼3 W/m2 less than the mean value, due to the positive skewness of the distribution. The near‐global mean TOA SW flux converted from MISR is about 7 W/m2 (∼7%) less than CERES measured flux during the last two decades. Surprisingly, a hemispheric asymmetry exists with TOA SW observations from the Terra platform. SH reflects 3.92 and 1.15 W/m2 more mean SW flux than NH, from MISR and CERES Single Scanner Footprint products, respectively. We can infer that the offsetting by morning clouds in the SH is greater than the effect of hemispheric imbalance of SW flux caused by different land masses in two hemispheres. While the characteristics of the two SW fluxes broadly agree with each other, differences in the regional PDF from two different SW fluxes are substantial over high cloud regions and high altitude regions. Our analysis shows that some parts of the different skewness from the two measurements may be attributed to the different calibration of the radiance anisotropy over high cloud scenes.
Galectin-1 activates carbonic anhydrase IX and modulates glioma metabolism
Authors: Guda Maheedhara R. , Tsung Andrew J. , Asuthkar Swapna , Velpula Kiran K. 
Published: June 2022

AbstractGalectins are a family of β-galactose-specific binding proteins residing within the cytosol or nucleus, with a highly conserved carbohydrate recognition domain across many species. Accumulating evidence shows that Galectin 1 (Gal-1) plays an essential role in cancer, and its expression correlates with tumor aggressiveness and progression. Our preliminary data showed Gal-1 promotes glioma stem cell (GSC) growth via increased Warburg effect. mRNA expression and clinical data were obtained from The Cancer Genome Atlas database. The immunoblot analysis conducted using our cohort of human glioblastoma patient specimens (hGBM), confirmed Gal-1 upregulation in GBM. GC/MS analysis to evaluate the effects of Gal-1 depletion showed elevated levels of α-ketoglutaric acid, and citric acid with a concomitant reduction in lactic acid levels. Using Biolog microplate-1 mitochondrial functional assay, we confirmed that the depletion of Gal-1 increases the expression levels of the enzymes from the TCA cycle, suggesting a reversal of the Warburg phenotype. Manipulation of Gal-1 using RNA interference showed reduced ATP, lactate levels, cell viability, colony-forming abilities, and increased expression levels of genes implicated in the induction of apoptosis. Gal-1 exerts its metabolic role via regulating the expression of carbonic anhydrase IX (CA-IX), a surrogate marker for hypoxia. CA-IX functions downstream to Gal-1, and co-immunoprecipitation experiments along with proximity ligation assays confirm that Gal-1 physically associates with CA-IX to regulate its expression. Further, silencing of Gal-1 in mice models showed reduced tumor burden and increased survival compared to the mice implanted with GSC controls. Further investigation of Gal-1 in GSC progression and metabolic reprogramming is warranted.
Allosteric inhibition of PPM1D serine/threonine phosphatase via an altered conformational state
Authors: Miller Peter G. , Sathappa Murugappan , Moroco Jamie A. , Jiang Wei , Qian Yue , Iqbal Sumaiya , Guo Qi , Giacomelli Andrew O. , Shaw Subrata , Vernier Camille , Bajrami Besnik , Yang Xiaoping , Raffier Cerise , Sperling Adam S. , Gibson Christopher J. , Kahn Josephine , Jin Cyrus , Ranaghan Matthew , Caliman Alisha , Brousseau Merissa , Fischer Eric S. , Lintner Robert , Piccioni Federica , Campbell Arthur J. , Root David E. , Garvie Colin W. , Ebert Benjamin L. 
Published: June 2022

AbstractPPM1D encodes a serine/threonine phosphatase that regulates numerous pathways including the DNA damage response and p53. Activating mutations and amplification of PPM1D are found across numerous cancer types. GSK2830371 is a potent and selective allosteric inhibitor of PPM1D, but its mechanism of binding and inhibition of catalytic activity are unknown. Here we use computational, biochemical and functional genetic studies to elucidate the molecular basis of GSK2830371 activity. These data confirm that GSK2830371 binds an allosteric site of PPM1D with high affinity. By further incorporating data from hydrogen deuterium exchange mass spectrometry and sedimentation velocity analytical ultracentrifugation, we demonstrate that PPM1D exists in an equilibrium between two conformations that are defined by the movement of the flap domain, which is required for substrate recognition. A hinge region was identified that is critical for switching between the two conformations and was directly implicated in the high-affinity binding of GSK2830371 to PPM1D. We propose that the two conformations represent active and inactive forms of the protein reflected by the position of the flap, and that binding of GSK2830371 shifts the equilibrium to the inactive form. Finally, we found that C-terminal truncating mutations proximal to residue 400 result in destabilization of the protein via loss of a stabilizing N- and C-terminal interaction, consistent with the observation from human genetic data that nearly all PPM1D mutations in cancer are truncating and occur distal to residue 400. Taken together, our findings elucidate the mechanism by which binding of a small molecule to an allosteric site of PPM1D inhibits its activity and provides insights into the biology of PPM1D.
On-chip bacterial foraging training in silicon photonic circuits for projection-enabled nonlinear classification
Authors: Cong Guangwei , Yamamoto Noritsugu , Inoue Takashi , Maegami Yuriko , Ohno Morifumi , Kita Shota , Namiki Shu , Yamada Koji 
Published: June 2022

AbstractOn-chip training remains a challenging issue for photonic devices to implement machine learning algorithms. Most demonstrations only implement inference in photonics for offline-trained neural network models. On the other hand, artificial neural networks are one of the most deployed algorithms, while other machine learning algorithms such as supporting vector machine (SVM) remain unexplored in photonics. Here, inspired by SVM, we propose to implement projection-based classification principle by constructing nonlinear mapping functions in silicon photonic circuits and experimentally demonstrate on-chip bacterial foraging training for this principle to realize single Boolean logics, combinational Boolean logics, and Iris classification with ~96.7 − 98.3 per cent accuracy. This approach can offer comparable performances to artificial neural networks for various benchmarks even with smaller scales and without leveraging traditional activation functions, showing scalability advantage. Natural-intelligence-inspired bacterial foraging offers efficient and robust on-chip training, and this work paves a way for photonic circuits to perform nonlinear classification.
Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage
Authors: Lachat Joy , Pascault Alice , Thibaut Delphine , Le Borgne Rémi , Verbavatz Jean-Marc , Weiner Allon 
Published: June 2022

AbstractThe opportunistic fungal pathogen Candida albicans is normally commensal, residing in the mucosa of most healthy individuals. In susceptible hosts, its filamentous hyphal form can invade epithelial layers leading to superficial or severe systemic infection. Although invasion is mainly intracellular, it causes no apparent damage to host cells at early stages of infection. Here, we investigate C. albicans invasion in vitro using live-cell imaging and the damage-sensitive reporter galectin-3. Quantitative single cell analysis shows that invasion can result in host membrane breaching at different stages and host cell death, or in traversal of host cells without membrane breaching. Membrane labelling and three-dimensional ‘volume’ electron microscopy reveal that hyphae can traverse several host cells within trans-cellular tunnels that are progressively remodelled and may undergo ‘inflations’ linked to host glycogen stores. Thus, C. albicans early invasion of epithelial tissues can lead to either host membrane breaching or trans-cellular tunnelling.
86Kr excess and other noble gases identify a billion-year-old radiogenically-enriched groundwater system
Authors: Warr O. , Ballentine C. J. , Onstott T. C. , Nisson D. M. , Kieft T. L. , Hillegonds D. J. , Sherwood Lollar B. 
Published: June 2022

AbstractDeep within the Precambrian basement rocks of the Earth, groundwaters can sustain subsurface microbial communities, and are targets of investigation both for geologic storage of carbon and/or nuclear waste, and for new reservoirs of rapidly depleting resources of helium. Noble gas-derived residence times have revealed deep hydrological settings where groundwaters are preserved on millions to billion-year timescales. Here we report groundwaters enriched in the highest concentrations of radiogenic products yet discovered in fluids, with an associated86Kr excess in the free fluid, and residence times >1 billion years. This brine, from a South African gold mine 3 km below surface, demonstrates that ancient groundwaters preserved in the deep continental crust on billion-year geologic timescales may be more widespread than previously understood. The findings have implications beyond Earth, where on rocky planets such as Mars, subsurface water may persist on long timescales despite surface conditions that no longer provide a habitable zone.
Comparative genomics of Acinetobacter baumannii and therapeutic bacteriophages from a patient undergoing phage therapy
Authors: Liu Mei , Hernandez-Morales Adriana , Clark James , Le Tram , Biswas Biswajit , Bishop-Lilly Kimberly A. , Henry Matthew , Quinones Javier , Voegtly Logan J. , Cer Regina Z. , Hamilton Theron , Schooley Robert T. , Salka Scott , Young Ry , Gill Jason J. 
Published: June 2022

AbstractIn 2016, a 68-year-old patient with a disseminated multidrug-resistant Acinetobacter baumannii infection was successfully treated using lytic bacteriophages. Here we report the genomes of the nine phages used for treatment and three strains of A. baumannii isolated prior to and during treatment. The phages used in the initial treatment are related, T4-like myophages. Analysis of 19 A. baumannii isolates collected before and during phage treatment shows that resistance to the T4-like phages appeared two days following the start of treatment. We generate complete genomic sequences for three A. baumannii strains (TP1, TP2 and TP3) collected before and during treatment, supporting a clonal relationship. Furthermore, we use strain TP1 to select for increased resistance to five of the phages in vitro, and identify mutations that are also found in phage-insensitive isolates TP2 and TP3 (which evolved in vivo during phage treatment). These results support that in vitro investigations can produce results that are relevant to the in vivo environment.
Mangrove dispersal disrupted by projected changes in global seawater density
Authors: Van der Stocken Tom , Vanschoenwinkel Bram , Carroll Dustin , Cavanaugh Kyle C. , Koedam Nico 
Published: June 2022

AbstractThe degree to which the distribution of mangrove forests will be impacted by climate change depends on the dispersal and establishment of sea-faring propagules, which drive forest rejuvenation, gene flow and range expansion. Climate change affects sea surface density via changes in temperature and salinity. However, these changes have not been mapped and it remains unclear how these factors may impact mangrove propagule dispersal. Here, we provide evidence for strong warming of coastal mangrove waters and elevated geographic variability in surface ocean density under representative concentration pathway RCP 8.5 by 2100. The largest changes will occur in the Indo West Pacific region, the primary hotspot of mangrove diversity. By comparing propagule densities to predicted sea surface density, we assessed potential effects on mangrove propagule dispersal. In the future, a warmer and fresher ocean is likely to alter dispersal trajectories of mangrove propagules and increase rates of sinking in unsuitable offshore locations, potentially reducing the resilience of mangrove forests.
Global patterns of daily CO2 emissions reductions in the first year of COVID-19
Authors: Liu Zhu , Deng Zhu , Zhu Biqing , Ciais Philippe , Davis Steven J. , Tan Jianguang , Andrew Robbie M. , Boucher Olivier , Arous Simon Ben , Canadell Josep G. , Dou Xinyu , Friedlingstein Pierre , Gentine Pierre , Guo Rui , Hong Chaopeng , Jackson Robert B. , Kammen Daniel M. , Ke Piyu , Le Quéré Corinne , Monica Crippa , Janssens-Maenhout Greet , Peters Glen P. , Tanaka Katsumasa , Wang Yilong , Zheng Bo , Zhong Haiwang , Sun Taochun , Schellnhuber Hans Joachim 
Published: June 2022

AbstractDay-to-day changes in CO2emissions from human activities, in particular fossil-fuel combustion and cement production, reflect a complex balance of influences from seasonality, working days, weather and, most recently, the COVID-19 pandemic. Here, we provide a daily CO2emissions dataset for the whole year of 2020, calculated from inventory and near-real-time activity data. We find a global reduction of 6.3% (2,232 MtCO2) in CO2emissions compared with 2019. The drop in daily emissions during the first part of the year resulted from reduced global economic activity due to the pandemic lockdowns, including a large decrease in emissions from the transportation sector. However, daily CO2emissions gradually recovered towards 2019 levels from late April with the partial reopening of economic activity. Subsequent waves of lockdowns in late 2020 continued to cause smaller CO2reductions, primarily in western countries. The extraordinary fall in emissions during 2020 is similar in magnitude to the sustained annual emissions reductions necessary to limit global warming at 1.5 °C. This underscores the magnitude and speed at which the energy transition needs to advance.
Carbon fixation rates in groundwater similar to those in oligotrophic marine systems
Authors: Overholt Will A. , Trumbore Susan , Xu Xiaomei , Bornemann Till L. V. , Probst Alexander J. , Krüger Markus , Herrmann Martina , Thamdrup Bo , Bristow Laura A. , Taubert Martin , Schwab Valérie F. , Hölzer Martin , Marz Manja , Küsel Kirsten 
Published: June 2022

AbstractThe terrestrial subsurface contains nearly all of Earth’s freshwater reserves and harbours the majority of our planet’s total prokaryotic biomass. Although genetic surveys suggest these organisms rely on in situ carbon fixation, rather than the photosynthetically derived organic carbon transported from surface environments, direct measurements of carbon fixation in the subsurface are absent. Using an ultra-low level14C-labelling technique, we estimate in situ carbon fixation rates in a carbonate aquifer. We find these rates are similar to those measured in oligotrophic marine surface waters and up to six-fold greater than those observed in the lower euphotic zone. Our empirical carbon fixation rates agree with nitrification rate data. Metagenomic analyses reveal abundant putative chemolithoautotrophic members of an uncharacterized order of Nitrospiria that may be behind the carbon fixation. On the basis of our determined carbon fixation rates, we conservatively extrapolate global primary production in carbonate groundwaters (10% of global reserves) to be 0.11 Pg carbon per year. These rates fall within the range found for oligotrophic marine surface waters, indicating a substantial contribution of in situ primary production to subsurface ecosystem processes. We further suggest that, just as phototrophs are for marine biogeochemical cycling, such subsurface carbon fixation is potentially foundational to subsurface trophic webs.
Advancing early warning capabilities with CHIRPS-compatible NCEP GEFS precipitation forecasts
Authors: Harrison Laura , Landsfeld Martin , Husak Greg , Davenport Frank , Shukla Shraddhanand , Turner William , Peterson Pete , Funk Chris 
Published: June 2022

AbstractCHIRPS-GEFS is an operational data set that provides daily bias-corrected forecasts for next 1-day to ~15-day precipitation totals and anomalies at a quasi-global 50-deg N to 50-deg S extent and 0.05-degree resolution. These are based on National Centers for Environmental Prediction (NCEP) Global Ensemble Forecast System version 12 (GEFS v12) precipitation forecasts. CHIRPS-GEFS forecasts are compatible with Climate Hazards center InfraRed Precipitation with Stations (CHIRPS) data, which is actively used for drought monitoring, early warning, and near real-time impact assessments. A rank-based quantile matching procedure is used to transform GEFS v12 “reforecast” and “real-time” forecast ensemble means to CHIRPS spatial-temporal characteristics. Matching distributions to CHIRPS makes forecasts better reflect local climatology at finer spatial resolution and reduces moderate-to-large forecast errors. As shown in this study, having a CHIRPS-compatible version of the latest generation of NCEP GEFS forecasts enables rapid assessment of current forecasts and local historical context. CHIRPS-GEFS effectively bridges the gap between observations and weather predictions, increasing the value of both by connecting monitoring resources (CHIRPS) with interoperable forecasts.
Earliest giant panda false thumb suggests conflicting demands for locomotion and feeding
Authors: Wang Xiaoming , Su Denise F. , Jablonski Nina G. , Ji Xueping , Kelley Jay , Flynn Lawrence J. , Deng Tao 
Published: June 2022

AbstractOf the many peculiarities that enable the giant panda (Ailuropoda melanoleuca), a member of the order Carnivora, to adapt to life as a dedicated bamboo feeder, its extra “thumb” is arguably the most celebrated yet enigmatic. In addition to the normal five digits in the hands of most mammals, the giant panda has a greatly enlarged wrist bone, the radial sesamoid, that acts as a sixth digit, an opposable “thumb” for manipulating bamboo. We report the earliest enlarged radial sesamoid, already a functional opposable “thumb,” in the ancestral panda Ailurarctos from the late Miocene site of Shuitangba in Yunnan Province, China. However, since the late Miocene, the “thumb” has not enlarged further because it must be balanced with the constraints of weight bearing while walking in a plantigrade posture. This morphological adaptation in panda evolution thus reflects a dual function of the radial sesamoid for both bamboo manipulation and weight distribution. The latter constraint could be the main reason why the panda’s false thumb never evolved into a full digit. This crude “thumb” suggests that the origin of the panda’s dedicated bamboo diet goes back to as early as 6–7 Ma.