bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi‐Scale Kelvin‐Helmholtz Instability Dynamics Observed by PMC Turbo on 12 July 2018: 2. DNS Modeling of KHI Dynamics and PMC Responses
Authors: Fritts David C. , Wang Ling , Lund Thomas S. , Thorpe S. A. , Kjellstrand C. Bjorn , Kaifler Bernd , Kaifler Natalie 
Published: August 2022

AbstractKjellstrand et al. (2022), https://10.1029/2021JD036232 describes the evolution and dynamics of a strong, large‐scale Kelvin‐Helmholtz instability (KHI) event observed in polar mesospheric clouds (PMCs) on 12 July 2018 by high‐resolution imagers aboard the PMC Turbulence (PMC Turbo) stratospheric long‐duration balloon experiment. The imaging provides evidence of KH billow interactions and instabilities that are strongly influenced by gravity waves at larger scales. Specific features include initially separated regions of KHI, secondary convective and KH instabilities of individual billows, and “tubes” and “knots” that arise where billow cores are mis‐aligned or discontinuous along their axes. This study describes a direct numerical simulation of KH billow interactions in a periodic domain seeded with random initial noise that enables excitation of multiple KH billows exhibiting variable phase structures that capture multiple features of the observed KHI dynamics. Variable KH billow phases along their axes yield initial vortex tubes having diagonal alignments that link adjacent, but mis‐aligned, billow cores. Weak initial vortex tubes and billow cores having nearly orthogonal alignments amplify, interact strongly, and drive intense vortex knots at these sites. These vortex tube and knot (T&K) dynamics excite “twist waves” that unravel the initial vortex tubes, and drive increasingly strong vortex interactions and a cascade of energy and enstrophy to successively smaller scales in the turbulence inertial range. The implications of T&K dynamics are much more rapid and intense breakdown and decay of the KH billows, and significantly enhanced energy dissipation rates, where these interactions occur.
Carbon to Nitrogen Uptake Ratios Observed Across the Southern Ocean by the SOCCOM Profiling Float Array
Authors: Johnson Kenneth S. , Mazloff Matthew R. , Bif Mariana B. , Takeshita Yuichiro , Jannasch Hans W. , Maurer Tanya L. , Plant Joshua N. , Verdy Ariane , Walz Peter M. , Riser Stephen C. , Talley Lynne D. 
Published: August 2022

AbstractMeasurements of pH and nitrate from the Southern Ocean Carbon and Climate Observations and Modeling array of profiling floats were used to assess the ratios of dissolved inorganic carbon (DIC) and nitrate (NO3) uptake during the spring to summer bloom period throughout the Southern Ocean. Two hundred and forty‐three bloom periods were observed by 115 floats from 30°S to 70°S. Similar calculations were made using the Takahashi surface DIC and nitrate climatology. To separate the effects of atmospheric CO2 exchange and mixing from phytoplankton uptake, the ratios of changes in DIC to nitrate of surface waters (ΔDIC/ΔNO3) were computed in the Biogeochemical Southern Ocean State Estimate (B‐SOSE) model. Phytoplankton uptake of DIC and nitrate are fixed in B‐SOSE at the Redfield Ratio (RR; 6.6 mol C/mol N). Deviations in the B‐SOSE ΔDIC/ΔNO3 must be due to non‐biological effects of CO2 gas exchange and mixing. ΔDIC/ΔNO3 values observed by floats and in the Takahashi climatology were corrected for the non‐biological effects using B‐SOSE. The corrected, in situ biological uptake ratio (C:N) occurs at values similar to the RR, with two major exceptions. North of 40°S biological DIC uptake is observed with little or no change in nitrate giving high C:N. In the latitude band at 55°S, the Takahashi data give a low C:N value, while floats are high. This may be due to a change in CO2 air‐sea exchange in this region from uptake during the Takahashi reference year of 2005 to outgassing of CO2 during the years sampled by floats.
New View of the Lunar Silicic Volcanism in the Mons Hansteen: Formation and Origins
Authors: Qiu Denggao , Ye Mao , Yan Jianguo , Zheng Chong , Xiao Zhiyong , Zhang Qing , Gao Wutong , Liu Lu , Li Fei 
Published: August 2022

AbstractSilicic volcanos with high‐thorium (Th), low FeO and TiO2 contents are rare on the Moon, but exploring the formation and origins of silicic volcanos can help us to understand the complex process of lunar crustal evolution. The origins of silicic volcanic Mons Hansteen complex on the southern edge of the Oceanus Procellarum (50.2°W and 12.2°S) are enigmatic. Here we use recent high‐resolution topographic data and gravity data to assess the morphology and shallow tectonic features of this complex to investigate its formation and evolution. The results from our crater size‐frequency distribution model show that the silicic volcanism in Mons Hansteen occurred as early as ∼3.8 Ga. A mafic intrusion beneath the Mons Hansteen originated from the Crater Billy impact event. The magma rose to within ∼11 km beneath the lunar surface causing the crust to melt and develop into silicic magma. The silicic magma erupted onto the lunar surface and was eventually covered by younger mare basalt. We conclude that silicic volcanos are older than we previously thought. Geophysical research on the Mons Hansteen area provides a new perspective thus supplementing and extending our knowledge of the silicic volcanism.
Risk of Climate Change for Hydroelectricity Production in China Is Small but Significant Reductions Cannot Be Precluded for More Than a Third of the Installed Capacity
Authors: Wan Wenhua , Döll Petra , Zheng Hang 
Published: August 2022

AbstractTo support the implementation of China's carbon neutrality target, this study assesses the impact of future climate change on hydroelectricity production (HP) in China. We developed a hydropower database for mainland China, which covers 92% of the installed capacity indicated by national statistics (352 GW in 2018). An HP model was applied to simulate monthly HP by 3,521 conventional plants at different levels of global warming (1°, 1.5°, 2°, and 3°C above preindustrial) using an ensemble of monthly streamflow time series computed by five global hydrological models (GHMs), where each global hydrological model was driven by four global climate models (GCMs). With a probability of 80%, limiting global warming to 1.5°C will constrain changes of national HP to the range of −1.4%–2.5% as compared to near‐present climate (1°C). In case of a 3°C world, this range more than doubles. HP is projected to likely increase in the two provinces Sichuan and Yunnan that encompass more than half of China's installed capacity. However, with a probability of about 10%, HP may decrease in a 3°C world by more than 15% in five provinces, and 40% of the installed capacity may suffer from an HP decrease of more than 5%. The uncertainty of projected HP changes is mainly due to the GCMs. We found that relative changes in annual HP and streamflow often differ in magnitude and direction as HP depends on seasonal streamflow patterns and installed capacities that determine the maximum flow through the turbines.
Prenatal dexamethasone exposure alters effort decision making and triggers nucleus accumbens and anterior cingulate cortex functional changes in male rats
Authors: Domingues Ana Verónica , Coimbra Bárbara , Correia Raquel , Deseyve Catarina , Vieitas-Gaspar Natacha , Floresco Stan B. , Sousa Nuno , Soares-Cunha Carina , Rodrigues Ana João 
Published: August 2022

AbstractDaily, individuals select actions based on cost-benefit to allocate resources into goal-directed actions. Different brain regions coordinate this complex decision, including the nucleus accumbens (NAc), anterior cingulate cortex (ACC), and ventral tegmental area (VTA). In utero exposure to synthetic glucocorticoids (iuGC), such as dexamethasone, triggers prominent motivation deficits but the impact of this exposure in the ACC-NAc and/or ACC-VTA circuits is unknown. Here, we show that iuGC exposure causes decreased motivation for natural rewards (food) and impaired effort-based decision-making. Importantly, reduced neuronal activation (number of c-fos+ neurons) was observed in the NAc core and ACC of iuGC rats in comparison to CTR rats after performing the effort-based decision-making task. In addition, iuGC treatment led to increased NAc and ACC basal neuronal activity. Electrophysiological recordings during optogenetic modulation of ACC terminals in the NAc revealed that the ACC-NAc circuit is dysfunctional in iuGC animals. These data suggest that iuGC animals present motivational and effort-based decision-making deficits that can be associated with the observed ACC-NAc dysfunction.
Associations of genetic liability for Alzheimer’s disease with cognition and eye movements in a large, population-based cohort study
Authors: Coors Annabell , Imtiaz Mohammed-Aslam , Boenniger Meta M. , Aziz N. Ahmad , Ettinger Ulrich , Breteler Monique M. B. 
Published: August 2022

AbstractTo identify cognitive measures that may be particularly sensitive to early cognitive decline in preclinical Alzheimer’s disease (AD), we investigated the relation between genetic risk for AD and cognitive task performance in a large population-based cohort study. We measured performance on memory, processing speed, executive function, crystallized intelligence and eye movement tasks in 5182 participants of the Rhineland Study, aged 30 to 95 years. We quantified genetic risk for AD by creating three weighted polygenic risk scores (PRS) based on the genome-wide significant single-nucleotide polymorphisms coming from three different genetic association studies. We assessed the relation of AD PRS with cognitive performance using generalized linear models. Three PRS were associated with lower performance on the Corsi forward task, and two PRS were associated with a lower probability of correcting antisaccade errors, but none of these associations remained significant after correction for multiple testing. Associations between age and trail-making test A (TMT-A) performance were modified by AD genetic risk, with individuals at high genetic risk showing the strongest association. We conclude that no single measure of our cognitive test battery robustly captures genetic liability for AD as quantified by current PRS. However, Corsi forward performance and the probability of correcting antisaccade errors may represent promising candidates whose ability to capture genetic liability for AD should be investigated further. Additionally, our finding on TMT-A performance suggests that processing speed represents a sensitive marker of AD genetic risk in old age and supports the processing speed theory of age-related cognitive decline.
Nuclear import of PTPN18 inhibits breast cancer metastasis mediated by MVP and importin β2
Authors: Wang Tao , Ba Xinlei , Zhang Xiaonan , Zhang Na , Wang Guowen , Bai Bin , Li Tong , Zhao Jiahui , Zhao Yanjiao , Yu Yang , Wang Bing 
Published: August 2022

AbstractDistant metastasis is the primary cause of breast cancer-associated death. The existing information, such as the precise molecular mechanisms and effective therapeutic strategies targeting metastasis, is insufficient to combat breast cancer. This study demonstrates that the protein tyrosine phosphatase PTPN18 is downregulated in metastatic breast cancer tissues and is associated with better metastasis-free survival. Ectopic expression of PTPN18 inhibits breast cancer cell metastasis. PTPN18 is translocated from the cytoplasm to the nucleus by MVP and importin β2 in breast cancer. Then, nuclear PTPN18 dephosphorylates ETS1 and promotes its degradation. Moreover, nuclear PTPN18 but not cytoplasmic PTPN18 suppresses transforming growth factor-β signaling and epithelial-to-mesenchymal transition by targeting ETS1. Our data highlight PTPN18 as a suppressor of breast cancer metastasis and provide an effective antimetastatic therapeutic strategy.
Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma
Authors: Sun Qi , Wang Yujie , Ji Hong , Sun Xiaoting , Xie Sisi , Chen Longtian , Li Sen , Zeng Weifan , Chen Ruibo , Tang Qi , Zuo Ji , Hou Likun , Hosaka Kayoko , Lu Yongtian , Liu Ying , Ye Ying , Yang Yunlong 
Published: August 2022

AbstractNasopharyngeal carcinoma (NPC) clinical trials show that antiangiogenic drugs (AADs) fail to achieve the expected efficacy, and combining AAD with chemoradiotherapy does not show superiority over chemoradiotherapy alone. Accumulating evidence suggests the intrinsic AAD resistance in NPC patients with poorly understood molecular mechanisms. Here, we describe NPC-specific FGF-2 expression-triggered, VEGF-independent angiogenesis as a mechanism of AAD resistance. Angiogenic factors screening between AAD-sensitive cancer type and AAD-resistant NPC showed high FGF-2 expression in NPC in both xenograft models and clinical samples. Mechanistically, the FGF-2-FGFR1-MYC axis drove endothelial cell survival and proliferation as an alternative to VEGF-VEGFR2-MYC signaling. Genetic knockdown of FGF-2 in NPC tumor cells reduced tumor angiogenesis, enhanced AAD sensitivity, and reduced pulmonary metastasis. Moreover, lenvatinib, an FDA recently approved multi-kinase inhibitor targeting both VEGFR2 and FGFR1, effectively inhibits the tumor vasculature, and exhibited robust anti-tumor effects in NPC-bearing nude mice and humanized mice compared with an agent equivalent to bevacizumab. These findings provide mechanistic insights on FGF-2 signaling in the modulation of VEGF pathway activation in the NPC microenvironment and propose an effective NPC-targeted therapy by using a clinically available drug.
Reactive oxygen species-generating hydrogel platform for enhanced antibacterial therapy
Authors: Hwang Changha , Choi Min-Ha , Kim Hyoun-Ee , Jeong Seol-Ha , Park Ji-Ung 
Published: August 2022

AbstractZinc oxide nanoparticles (ZnO) have attracted much attention as promising antibacterial agents due to their ability to generate reactive oxygen species (ROS) that effectively eliminate bacteria. However, when they are delivered inside the body, this distinct characteristic of ROS is restricted due to the limited penetration depth of external light, which is required for the photocatalysis of particles. To produce ROS without any light source when the particles are implanted, we introduced catechol-ZnO complexes to a hyaluronic acid (HA) hydrogel platform, which can self-generate sufficient ROS in the bacteria-infected tissue. Catechol-ZnO complexes enhanced ROS generation via electron transfer from the formation of complexes and o-semiquinone, and a hydrogel structure was created by coordinate bonds between functionalized catechol groups in HA and ZnO simultaneously. This hydrogel demonstrated different behaviors in terms of physical properties compared to chemically cross-linked HA hydrogels containing ZnO. This hydrogel showed a higher swelling ratio, enzymatic degradation resistance, and tissue adhesive strength. Enhanced ROS generation was confirmed using electron paramagnetic resonance (EPR), H2O2 concentration, glutathione depletion, and intracellular ROS detection. The improved antibacterial performance of hydrogels from ROS production was also confirmed through in vitro bacterial testing against two bacterial strains, E. coli and S. aureus. Furthermore, an in vivo experiment using an infected mouse model to analyze colony formation, histologic analysis, and hematological inflammatory markers revealed the effective antibacterial effects of catechol-ZnO complexes. Overall, the potential of the hydrogel created via catechol-ZnO complexes for antibacterial therapy was demonstrated through the capability to enhance ROS generation and eradicate bacteria.
Moisture-triggered fast crystallization enables efficient and stable perovskite solar cells
Authors: Liu Kaikai , Luo Yujie , Jin Yongbin , Liu Tianxiao , Liang Yuming , Yang Liu , Song Peiquan , Liu Zhiyong , Tian Chengbo , Xie Liqiang , Wei Zhanhua 
Published: August 2022

AbstractUnderstanding the function of moisture on perovskite is challenging since the random environmental moisture strongly disturbs the perovskite structure. Here, we develop various N2-protected characterization techniques to comprehensively study the effect of moisture on the efficient cesium, methylammonium, and formamidinium triple-cation perovskite (Cs0.05FA0.75MA0.20)Pb(I0.96Br0.04)3. In contrast to the secondary measurements, the established air-exposure-free techniques allow us directly monitor the influence of moisture during perovskite crystallization. We find a controllable moisture treatment for the intermediate perovskite can promote the mass transportation of organic salts, and help them enter the buried bottom of the films. This process accelerates the quasi-solid-solid reaction between organic salts and PbI2, enables a spatially homogeneous intermediate phase, and translates to high-quality perovskites with much-suppressed defects. Consequently, we obtain a champion device efficiency of approaching 24% with negligible hysteresis. The devices exhibit an average T80-lifetime of 852 h (maximum 1210 h) working at the maximum power point.
Crystal structures reveal catalytic and regulatory mechanisms of the dual-specificity ubiquitin/FAT10 E1 enzyme Uba6
Authors: Yuan Lingmin , Gao Fei , Lv Zongyang , Nayak Digant , Nayak Anindita , Santos Bury Priscila dos , Cano Kristin E. , Jia Lijia , Oleinik Natalia , Atilgan Firdevs Cansu , Ogretmen Besim , Williams Katelyn M. , Davies Christopher , El Oualid Farid , Wasmuth Elizabeth V. , Olsen Shaun K. 
Published: August 2022

AbstractThe E1 enzyme Uba6 initiates signal transduction by activating ubiquitin and the ubiquitin-like protein FAT10 in a two-step process involving sequential catalysis of adenylation and thioester bond formation. To gain mechanistic insights into these processes, we determined the crystal structure of a human Uba6/ubiquitin complex. Two distinct architectures of the complex are observed: one in which Uba6 adopts an open conformation with the active site configured for catalysis of adenylation, and a second drastically different closed conformation in which the adenylation active site is disassembled and reconfigured for catalysis of thioester bond formation. Surprisingly, an inositol hexakisphosphate (InsP6) molecule binds to a previously unidentified allosteric site on Uba6. Our structural, biochemical, and biophysical data indicate that InsP6 allosterically inhibits Uba6 activity by altering interconversion of the open and closed conformations of Uba6 while also enhancing its stability. In addition to revealing the molecular mechanisms of catalysis by Uba6 and allosteric regulation of its activities, our structures provide a framework for developing Uba6-specific inhibitors and raise the possibility of allosteric regulation of other E1s by naturally occurring cellular metabolites.
Immune-mediated tubule atrophy promotes acute kidney injury to chronic kidney disease transition
Authors: Xu Leyuan , Guo Jiankan , Moledina Dennis G. , Cantley Lloyd G. 
Published: August 2022

AbstractIncomplete repair after acute kidney injury can lead to development of chronic kidney disease. To define the mechanism of this response, we compared mice subjected to identical unilateral ischemia-reperfusion kidney injury with either contralateral nephrectomy (where tubule repair predominates) or contralateral kidney intact (where tubule atrophy predominates). By day 14, the kidneys undergoing atrophy had more macrophages with higher expression of chemokines, correlating with a second wave of proinflammatory neutrophil and T cell recruitment accompanied by increased expression of tubular injury genes and a decreased proportion of differentiated tubules. Depletion of neutrophils and T cells after day 5 reduced tubular cell loss and associated kidney atrophy. In kidney biopsies from patients with acute kidney injury, T cell and neutrophil numbers negatively correlated with recovery of estimated glomerular filtration rate. Together, our findings demonstrate that macrophage persistence after injury promotes a T cell- and neutrophil-mediated proinflammatory milieu and progressive tubule damage.
Tethering of cellulose synthase to microtubules dampens mechano-induced cytoskeletal organization in Arabidopsis pavement cells
Authors: Schneider René , Ehrhardt David W. , Meyerowitz Elliot M. , Sampathkumar Arun 
Published: August 2022

AbstractMechanical forces control development in plants and animals, acting as cues in pattern formation and as the driving force of morphogenesis. In mammalian cells, molecular assemblies residing at the interface of the cell membrane and the extracellular matrix play an important role in perceiving and transmitting external mechanical signals to trigger physiological responses. Similar processes occur in plants, but there is little understanding of the molecular mechanisms and their genetic basis. Here, we show that the number and movement directions of cellulose synthase complexes (CSCs) at the plasma membrane vary during initial stages of development in the cotyledon epidermis of Arabidopsis, closely mirroring the microtubule organization. Uncoupling microtubules and CSCs resulted in enhanced microtubule co-alignment as caused by mechanical stimuli driven either by cell shape or by tissue-scale physical perturbations. Furthermore, micromechanical perturbation resulted in depletion of CSCs from the plasma membrane, suggesting a possible link between cellulose synthase removal from the plasma membrane and microtubule response to mechanical stimuli. Taken together, our results suggest that the interaction of cellulose synthase with cortical microtubules forms a physical continuum between the cell wall, plasma membrane and the cytoskeleton that modulates the mechano-response of the cytoskeleton.
An expanded whole-cell model of E. coli links cellular physiology with mechanisms of growth rate control
Authors: Ahn-Horst Travis A. , Mille Luis Santiago , Sun Gwanggyu , Morrison Jerry H. , Covert Markus W. 
Published: August 2022

AbstractGrowth and environmental responses are essential for living organisms to survive and adapt to constantly changing environments. In order to simulate new conditions and capture dynamic responses to environmental shifts in a developing whole-cell model of E. coli, we incorporated additional regulation, including dynamics of the global regulator guanosine tetraphosphate (ppGpp), along with dynamics of amino acid biosynthesis and translation. With the model, we show that under perturbed ppGpp conditions, small molecule feedback inhibition pathways, in addition to regulation of expression, play a role in ppGpp regulation of growth. We also found that simulations with dysregulated amino acid synthesis pathways provide average amino acid concentration predictions that are comparable to experimental results but on the single-cell level, concentrations unexpectedly show regular fluctuations. Additionally, during both an upshift and downshift in nutrient availability, the simulated cell responds similarly with a transient increase in the mRNA:rRNA ratio. This additional simulation functionality should support a variety of new applications and expansions of the E. coli Whole-Cell Modeling Project.