bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multilayered Ceramic Membrane with Ion Conducting Thin Layer Induced by Interface Reaction for Stable Hydrogen Production
Authors: He Guanghu , Lan Qianqian , Liu Mengke , Wu Guixuan , Dunin‐Borkowski Rafal E. , Jiang Heqing 
Published: November 2022

AbstractConventional methods for fabricating multilayered ceramic membranes with ion conducting dense thin layers are often cumbersome, costly, and limited by poor adhesion between layers. Inspired by the architectural structure of the rooted grasses in soil, here, we report an interface‐reaction‐induced reassembly approach for the direct fabrication of Ce0.9Gd0.1O2−δ (CGO) thin layers rooted in the parent multilayered ceramic membranes by only one firing step. The CGO dense layers are very thin, and adhered strongly to the parent support layer, ensuring low ionic transport resistance and structural integrity of the multilayered membranes. When using as an oxygen permeable membrane for upgrading fossil‐fuel‐derived hydrogen, it shows very long durability in harsh conditions containing H2O, CH4, H2, CO2 and H2S. Furthermore, our approach is highly scalable and applicable to a wide variety of ion conducting thin layers, including Y0.08Zr0.92O2−δ, Ce0.9Sm0.1O2−δ and Ce0.9Pr0.1O2−δ.
Imine Directed Cp*RhIII‐Catalyzed N−H Functionalization and Annulation with Amino Amides, Aldehydes, and Diazo Compounds
Authors: Zoll Adam J. , Molas Jenna C. , Mercado Brandon Q. , Ellman Jonathan A. 
Published: November 2022

AbstractA multicomponent annulation that proceeds by imine directed Cp*RhIII‐catalyzed N−H functionalization is disclosed. The transformation affords piperazinones displaying a range of functionality and is the first example of transition metal‐catalyzed multicomponent N−H functionalization. A broad range of readily available α‐amino amides, including those derived from glycine, α‐substituted, and α,α‐disubstituted amino acids, were effective inputs and enabled the incorporation of a variety of amino acid side chains with minimal racemization. Branched and unbranched alkyl aldehydes and various stabilized diazo compounds were also efficient reactants. The piperazinone products were further modified through efficient transformations. Mechanistic studies, including X‐ray crystallographic characterization of a catalytically competent five‐membered rhodacycle with imine and amide nitrogen chelation, provide support for the proposed mechanism.
Fluorine‐Directed Automated Mannoside Assembly
Authors: Teschers Charlotte S. , Gilmour Ryan 
Published: November 2022

AbstractAutomated glycan assembly (AGA) on solid support has become invaluable in reconciling the biological importance of complex carbohydrates with the persistent challenges associated with reproducible synthesis. Whilst AGA platforms have transformed the construction of many natural sugars, validation in the construction of well‐defined (site‐selectively modified) glycomimetics is in its infancy. Motivated by the importance of fluorination in drug discovery, the biomedical prominence of 2‐fluoro sugars and the remarkable selectivities observed in fluorine‐directed glycosylation, fluorine‐directed automated glycan assembly (FDAGA) is disclosed. This strategy leverages the fluorine atom for stereocontrolled glycosylation on solid support, thereby eliminating the reliance on O‐based directing groups. The logical design of C2‐fluorinated mannose building blocks, and their application in the fully (α‐)stereocontrolled automated assembly of linear and branched fluorinated oligomannosides, is disclosed. This operationally simple strategy can be integrated into existing AGA and post‐AGA protocols to augment the scope of programmed carbohydrate synthesis.
Effect of sodium dodecyl sulfate on shape‐memory properties of side‐chain crystalline hydrogel via micellar copolymerization
Authors: Chen Xi , Wang Shuaiyin , Yu Bowen , Li Jihui , Liu Lingzhi , Lu Ying , Xu Wenyuan , Liu Yongxin 
Published: November 2022

AbstractA chemical shape‐memory hydrogel containing crystalline structure is prepared via micellar copolymerization of hydrophilic monomer acrylamide (AM) and hydrophobic monomer octadecyl acrylate (C18) in a sodium dodecyl sulfate (SDS) solution. The influence of SDS on the shape‐memory behavior of hydrogel investigated by differential scanning calorimetry (DSC) and Temperature‐dependent X‐ray indicate that the melting and crystallization peaks derived from the thermal properties of C18 units is different from peaks corresponding to the pure SDS. In addition, the microstructure evolution information of hydrogels dose not change upon heating process, regardless of the presence or absence of SDS. Therefore, only the hydrophobic associations formed by C18 blocks play a decisive role in the shape memory function of hydrogel system. The difference between the transition process (from temporary shape to permanent shape) of hydrogel with and without SDS at different temperature is because that the hydrophobic region of side chain crystallization of SDS‐free hydrogel may escape the restriction of SDS and become more sensitive to temperature, they can preferentially return to their initial shape under the same time and temperature than SDS‐containing gels. Whether SDS exists only affect the speed of shape memory behavior, but not the microstructure evolution of hydrogel system.
Endosymbiotic ratchet accelerates divergence after organelle origin
Authors: Bhattacharya Debashish , Etten Julia Van , Benites L. Felipe , Stephens Timothy G. 
Published: November 2022

AbstractWe hypothesize that as one of the most consequential events in evolution, primary endosymbiosis accelerates lineage divergence, a process we refer to as the endosymbiotic ratchet. Our proposal is supported by recent work on the photosynthetic amoeba, Paulinella, that underwent primary plastid endosymbiosis about 124 Mya. This amoeba model allows us to explore the early impacts of photosynthetic organelle (plastid) origin on the host lineage. The current data point to a central role for effective population size (Ne) in accelerating divergence post‐endosymbiosis due to limits to dispersal and reproductive isolation that reduce Ne, leading to local adaptation. We posit that isolated populations exploit different strategies and behaviors and assort themselves in non‐overlapping niches to minimize competition during the early, rapid evolutionary phase of organelle integration. The endosymbiotic ratchet provides a general framework for interpreting post‐endosymbiosis lineage evolution that is driven by disruptive selection and demographic and population shifts. Also see the video abstract here: https://youtu.be/gYXrFM6Zz6Q
LC–MS/MS determination of HY072808, a novel candidate for treating atopic dermatitis, and its active metabolite: Application to a first‐in‐human pharmacokinetic study
Authors: Wu Jingying , Zheng Liang , Zhang Qian , Zhang Qin , Qin Huiling , Zhou Renpeng , Chu Zhaoxing , He Guangwei , Wang Ling , Hu Wei 
Published: November 2022

AbstractHY072808 is a novel phosphodiesterase 4 inhibitor currently under clinical development to treat atopic dermatitis. The first step is to address the pharmacokinetics and safety after topical administration of HY072808 ointments in healthy humans. In this study, we developed a highly sensitive liquid chromatography–tandem mass spectrometry method to determine plasma HY072808 and its active metabolite, ZZ24, in tiny amounts. The plasma samples were prepared using a simple liquid–liquid extraction method. Liquid chromatographic separation was achieved by gradient elution. The MS/MS quantification was performed in positive ion mode via multiple reaction monitoring. The method showed satisfactory linearity from 10 to 4,000 pg/ml for HY072808 and ZZ24. There was no significant interference from blank plasma. The method was validated for accuracy and precision, matrix effect and extraction recovery, dilution integrity, injection carryover and stability according to the related guidelines of the regulatory authorities. The HY072808 and ZZ24 concentrations in human plasma from a clinical trial were determined using this method. In conclusion, the validated method was robust and could be utilized to support the clinical development of HY072808.
A Novel Coenzyme A Analogue in the Anaerobic, Sulfate‐Reducing, Marine Bacterium Desulfobacula toluolica Tol2T
Authors: Bruns Stefan , Cakić Nevenka , Mitschke Nico , Kopke Bernd Johann , Rabus Ralf , Wilkes Heinz 
Published: November 2022

AbstractCoenzyme A (CoA) thioesters are formed during anabolic and catabolic reactions in every organism. Degradation pathways of growth‐supporting substrates in bacteria can be predicted by differential proteogenomic studies. Direct detection of proposed metabolites such as CoA thioesters by high‐performance liquid chromatography coupled with high‐resolution mass spectrometry can confirm the reaction sequence and demonstrate the activity of these degradation pathways. In the metabolomes of the anaerobic sulfate‐reducing bacterium Desulfobacula toluolica Tol2T grown with different substrates various CoA thioesters, derived from amino acid, fatty acid or alcohol metabolism, have been detected. Additionally, the cell extracts of this bacterium revealed a number of CoA analogues with molecular masses increased by 1 dalton. By comparing the chromatographic and mass spectrometric properties of synthetic reference standards with those of compounds detected in cell extracts of D. toluolica Tol2T and by performing co‐injection experiments, these analogues were identified as inosino‐CoAs. These CoA thioesters contain inosine instead of adenosine as the nucleoside. To the best of our knowledge, this finding represents the first detection of naturally occurring inosino‐CoA analogues.
Multifocal periapical cemental dysplasia in periodontal Ehlers–Danlos syndrome combined with leukoencephalopathy in the mutation of c.890G > a, G297D [pEDS]
Authors: Nilius Manfred , Nilius Minou Helene , Müller Charlotte , Lauer Guenter , Berit Koch , Marcus Kohlhaas 
Published: November 2022

AbstractPeriodontal Ehlers‐Danlos syndrome (pEDS) is a rare disorder caused by heterozygous mutations in complement 1 subunit genes C1R and C1S. To date, 148 cases have been described in the literature.We describe a case of a suspected de novo‐mutation of pEDS with generalized Periapical cemental dysplasia (PCD) and cerebral leukoencephalopathy.
OMIP‐086: Full spectrum flow cytometry for high‐dimensional immunophenotyping of mouse innate lymphoid cells
Authors: Mincham Kyle T. , Snelgrove Robert J. 
Published: November 2022

AbstractThis 25‐parameter, 22‐color full spectrum flow cytometry panel was designed and optimized for the comprehensive enumeration and functional characterization of innate lymphoid cell (ILC) subsets in mouse tissues. The panel presented here allows the discrimination of ILC progenitors (ILCP), ILC1, ILC2, NCR+ ILC3, NCR− ILC3, CCR6+ lymphoid tissue‐inducer (LTi)‐like ILC3 and mature natural killer (NK) cell populations. Further characterization of ILC and NK cell functional profiles in response to stimulation is provided by the inclusion of subset‐specific cytokine markers, and proliferation markers. Development and optimization of this panel was performed on freshly isolated cells from adult BALB/c lungs and small intestine lamina propria, and ex vivo stimulation with phorbol 12‐myrisate 13‐acetate, ionomycin, and pro‐ILC activating cytokines.
Chemical composition and trophic status of urban lakes of the Arctic zone of the Russian Federation (Murmansk)
Authors: Postevaya Marina , Dauvalter Vladimir , Slukovskii Zakhar , Kudryavtseva Lyubov 
Published: November 2022

AbstractThe chemical composition and seasonal dynamics of the water in Murmansk urban lakes (Murmansk region, Russia) under the anthropogenic load of varying intensity was analysed. The studies were conducted in the period from 2018 to 2020. The chemical composition of the water in the studied lakes differed significantly from that of the lakes located in the background territories of the region. High values of pH and mineralization, increased concentrations of major ions and elevated content of nitrogen and phosphorus compounds were recorded in the surface waters. The nutrient concentrations were subject to noticeable seasonal fluctuations, mainly due to their consumption by phytoplankton, as well as to the changes in the ratios of surface and underground runoff. Lake Ledovoe (the central part of Murmansk) was the most polluted among the studied lakes, and Lake Bolshoe (used for drinking supply of the city) was the least polluted. Assessing the trophic status of the Murmansk lakes showed that the studied lakes are mostly mesotrophic, except for Lake Bolshoe (oligotrophic) and Lake Ledovoe (hypertrophic). In most of the lakes, phosphorus was the limiting factor for the growth and development of phytoplankton. The data obtained can be useful for understanding the fundamental processes of water composition transformation in the urbanized territories of the Arctic regions of the world and for making managerial (practical) decisions to create a comfortable urban environment in the North of Russia.
The “fruit and whole‐grain” pattern is associated with a low prevalence of hypertriglyceridemia among middle and older‐aged Korean adults: Using Korea National Health and Nutrition Examination Survey 2013–2018 data
Authors: Park SoHyun , Chung Sangwon , Kim Seong‐Ah , Shin Sangah 
Published: November 2022

AbstractHypertriglyceridemia is a well‐known risk factor of various chronic diseases including diabetes mellitus, metabolic syndrome, obesity, and cardiovascular diseases. This study aimed to determine dietary patterns and explore the relationship between dietary patterns and hypertriglyceridemia in the Korean adult population. We utilized a cross‐sectional and nationally representative survey, the Korea National Health and Nutrition Examination Survey 2013–2018 database. From 47,217 subjects who participated in the survey between 2013 and 2018, only subjects over 40 years old were included. Subjects lacking 24‐h recall data and data on hypertriglyceridemia and body mass index, and who had implausible energy intake were excluded. A total of 19,806 participants' data were analyzed. Dietary data were based on 24‐h recall data, and dietary patterns were derived using factor analysis. Triglyceride levels greater than 200 mg/dl were considered hypertriglyceridemia, according to the Korean Society of Lipid and Atherosclerosis. Three dietary patterns— “oil and fats & seasoning”, “soybean paste and vegetable”, and “fruit and whole‐grain”— explained 7.9%, 6.3%, and 5.8% of variation in food intake, respectively. Comparing the lowest and highest dietary pattern score groups after adjusting for potential confounders revealed an inverse relationship between “fruit and whole‐grain” dietary pattern and hypertriglyceridemia in men (odds ratio [OR]: 0.61, 95% confidence interval [CI]: 0.45–0.82, p for trend <.0001); which was only marginal in women (OR: 0.78, 95% CI: 0.58–1.07, p for trend: .628). A diet containing high proportions of fruit and whole‐grain may have preventive effects on hypertriglyceridemia in middle and older aged Korean adults.
Provenance and sedimentation age of the Proterozoic clastic succession of the Garhwal‐Kumaon Lesser Himalaya, NW‐India: Clues from U–Pb zircon and Sr–Nd isotopes
Authors: Negi Manju , Saha Subhojit , Ghosh Sumit K. , Rai Santosh K. 
Published: November 2022

The paper presents data on the U–Pb chronology of detrital zircon grains and radiogenic isotopic composition (87Sr/86Sr, ɛNd) from the Proterozoic clastic successions of the Garhwal‐Kumaon Lesser Himalaya representing the extended northern Indian cratonic margin, NW India. The Proterozoic Lesser Himalayan Basin in Garhwal‐Kumaon Himalaya is divided into two sectors, namely, Inner Lesser Himalaya (ILH) and Outer Lesser Himalaya (OLH) by a tectonic boundary, namely the Tons Thrust (TT). Age distribution from inner and outer sectors of the Lesser Himalaya shows that the U–Pb chronology of most of these zircons provides Palaeoproterozoic (between 1.6 and 1.8 Ga) to Neoproterozoic (800 Ma) ages. The age data suggest sedimentation of the Rautgara Formation (Damtha Group) of ILH continued till the Neoproterozoic (~850 Ma), which was earlier regarded as ≤1.6 Ga. Tracking the detrital U–Pb zircon ages in the near adjacent cratonic parts point towards Aravalli Orogen as the major source region. Whole‐rock ƐNd(0) values for ILH rocks range from −37.6 to −14.6 and for OLH it ranges from −19.6 to −6.7. More negative ƐNd values along with dominance of Neoarchean‐Palaeoproterozoic ages in ILH indicates supply from more evolved protolith or recycled sources and less negative ƐNd values with major Neoproterozic zircon ages from OLH supports for less evolved source rock. The change from more negative ƐNd to less negative ƐNd values progressively upward the stratigraphy can be due to a shift in source with time. Both U–Pb zircon and ƐNd supports for a continuous sedimentation model, rule out the presence of ~500 Ma unconformity within the LH and argues for separate evolution of the Lesser Himalayan Basin on the trailing edge of the extended north Indian craton in “Columbia” configuration.
Guide to in‐field assessment of the painful equine eye
Authors: de Oliveira Chez , Dixon Sam 
Published: November 2022

Background: Using simple equipment and a systematic approach, it is possible to perform a thorough ocular equine examination in the field. Good‐quality images and videos can be obtained on most smartphones and can prove invaluable both for monitoring the progress of ocular disease, or when seeking referral or advice from an ophthalmologist. Even in cases where referral is not an option, a significant amount can be achieved in the field by the first‐opinion practitioner.Aim of the article: This article sets out a logical and systematic approach to the ocular examination, and outlines how to interpret the examination findings in order to arrive at an accurate diagnosis and instigate an initial treatment plan.
Genetic tools to dissect functions of long noncoding RNAs
Authors: Nawrocka Anna , Andric Vedrana , Shkumatava Alena 
Published: November 2022

AbstractLncRNAs represent an abundant group of noncoding transcripts, some of which carry out important regulatory functions. To survey the biological and molecular roles of lncRNAs, reliable strategies for their genetic inactivation are required. Several lncRNA features make them challenging to target by genome editing. First, lncRNA loci often span large genomic distances. As such, full or partial deletion alleles are not always easy to generate and interpret as they might affect DNA regulatory elements. Second, in contrast to proteins, lncRNA transcripts are usually resistant to the minimally invasive approach of point substitutions. Third, lncRNA sequences exhibit rapid evolutionary turnover, impeding prediction and targeting of the specific functional sequence elements. Nonetheless, advances in genome editing and comparative genomics have expanded the repertoire of genetic strategies to dissect lncRNA functions in model organisms and cell lines. In this review, we discuss several approaches that have been used to generate lncRNA mutant alleles, focusing on vertebrate lncRNAs. We also briefly highlight comparative genomics approaches to identify conserved lncRNA sequence motifs, which represent attractive target sequences to abrogate lncRNA functions and to pinpoint functional contributions of these elements.
Characterizing single extracellular vesicles by droplet barcode sequencing for protein analysis
Authors: Banijamali Mahsan , Höjer Pontus , Nagy Abel , Hååg Petra , Gomero Elizabeth Paz , Stiller Christiane , Kaminskyy Vitaliy O. , Ekman Simon , Lewensohn Rolf , Karlström Amelie Eriksson , Viktorsson Kristina , Ahmadian Afshin 
Published: November 2022

AbstractSmall extracellular vesicles (sEVs) have in recent years evolved as a source of biomarkers for disease diagnosis and therapeutic follow up. sEV samples derived from multicellular organisms exhibit a high heterogeneous repertoire of vesicles which current methods based on ensemble measurements cannot capture. In this work we present droplet barcode sequencing for protein analysis (DBS‐Pro) to profile surface proteins on individual sEVs, facilitating identification of sEV‐subtypes within and between samples. The method allows for analysis of multiple proteins through use of DNA barcoded affinity reagents and sequencing as readout. High throughput single vesicle profiling is enabled through compartmentalization of individual sEVs in emulsion droplets followed by droplet barcoding through PCR. In this proof‐of‐concept study we demonstrate that DBS‐Pro allows for analysis of single sEVs, with a mixing rate below 2%. A total of over 120,000 individual sEVs obtained from a NSCLC cell line and from malignant pleural effusion (MPE) fluid of NSCLC patients have been analyzed based on their surface proteins. We also show that the method enables single vesicle surface protein profiling and by extension characterization of sEV‐subtypes, which is essential to identify the cellular origin of vesicles in heterogenous samples.
Impact of moral injury and posttraumatic stress disorder on health care utilization and suicidality in rural and urban veterans
Authors: Parry Kimber J. , Hicken Bret L. , Chen Wei , Leng Jianwei , Allen Steven , Burningham Zachary 
Published: November 2022

AbstractThis study explored the impact of moral injury (MI) and posttraumatic stress disorder (PTSD) on health care utilization, mental health complexity, and suicidality in rural and urban veterans. Analyses combined data from the Salt Lake City PTSD Clinic Intake Database and the Department of Veterans Affairs Corporate Data Warehouse. Participants (N = 1,545; Mage = 45.9 years) were predominately male (88.3%) and White (87.8%). Adjusted analyses indicated associations between a 1‐unit increase in Moral Injury Events Scale (MIES) score and increased mental health complexity, RR = 1.01, 95% CI [1.01, 1.02], p < .001; psychotropic medication utilization, RR = 1.01, 95% CI [1.01, 1.03], p < .001; VA drug class count, RR = 1.01, 95% CI [1.00, 1.01], p = .030; outpatient utilization, RR = 1.01, 95% CI [1.01, 1.02], p < .001; and mental health outpatient utilization, RR = 1.01, 95% CI [1.00, 1.03], p < .001. For the MIES x PTSD interaction, all associations remained statistically significant with similar estimated effects. However, for rural veterans, this interaction did not significantly affect utilization. Among those with PTSD, a 1‐unit MIES increase was associated with an increased risk of suicidality, OR = 1.02, 95% CI [1.01, 1.04], and psychiatric admission, OR = 1.02, 95% CI [1.00, 1.04]. Findings suggest that higher MIES scores predict increased health care utilization and mental health complexity. Further, PTSD combined with higher MIES scores may increase the risk of suicidality and psychiatric admission. Rural veterans with PTSD and higher MIES scores may require additional outreach and intervention.