bluuebunny/crossref_metadata_2025_split huggingface dataset:
AMP-activated protein kinase (AMPK) is required for recovery from metabolic stress induced by ultrasound microbubble treatment
Authors: Lo Louis , Uchenunu Oro , Botelho Roberto J. , Antonescu Costin N. , Karshafian Raffi 
Published: March 2022

AbstractUltrasound and microbubbles (USMB) is a promising strategy for cancer therapy. USMB can induce a variety of effects on cells including transient formation of plasma membrane pores (sonoporation) and enhanced endocytosis, which enhance drug delivery, and can also lead to enhanced cell death. However, the outcomes of USMB on cell physiology are heterogeneous, in that USMB elicits cell death in a proportion of cells while exerting minimal effects on others. This suggests that mechanisms of adaptation following USMB allow some cells to survive and/or proliferate. The molecular mechanisms of adaptation to USMB-induced stress remain poorly understood, thus potentially hindering broad therapeutic applications of USMB. Herein, we used several triple negative breast cancer cells to study the effect of USMB-induced metabolite stress and the role of AMPK as a response to this stress. We found that USMB alters steady-state levels of amino acids, glycolytic intermediates, and citric acid cycle intermediates. USMB treatment acutely reduces ATP levels and stimulates AMP-activated protein kinase (AMPK) phosphorylation and activation. Further, AMPK is required to restore ATP levels in cells that survived the initial insult and support cell proliferation post-USMB treatment. These results suggest that AMPK and metabolic perturbations are likely determinants of the anti-neoplastic efficacy of USMB treatment.
Evaluation of N6-adenine DNA-immunoprecipitation-based genomic profiling in eukaryotes
Authors: Debo Brian M. , Mallory Ben , Stergachis Andrew B. 
Published: March 2022

ABSTRACTThe detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.
Predicting sex, age, general cognition and mental health with machine learning on brain structural connectomes
Authors: Yeung Hon Wah , Stolicyn Aleks , Buchanan Colin R. , Tucker-Drob Elliot M. , Bastin Mark E. , Luz Saturnino , McIntosh Andrew M. , Whalley Heather C. , Cox Simon R. , Smith Keith 
Published: March 2022

AbstractThere is increasing expectation that advanced, computationally expensive machine learning techniques, when applied to large population-wide neuroimaging datasets, will help to uncover key differences in the human brain in health and disease. We take a comprehensive approach to explore how multiple aspects of brain structural connectivity can predict sex, age, general cognitive function and general psychopathology, testing different machine learning algorithms from deep learning model (BrainNetCNN) to classical machine learning methods. We modelledN= 8, 183 structural connectomes from UK Biobank using six different structural network weightings obtained from diffusion MRI. Streamline count generally provided highest prediction accuracies in all prediction tasks. Deep learning did not improve on prediction accuracies from simpler linear models. Further, high correlations between gradient attribution coefficients from deep learning and model coefficients from linear models suggested the models ranked the importance of features in similar ways, which indirectly suggested the similarity in models’ strategies for making predictive decision to some extent. This highlights that model complexity is unlikely to improve detection of associations between structural connectomes and complex phenotypes with the current sample size.
Optimization of dynamic soaring in a flap-gliding seabird and its impacts on large-scale distribution at sea
Authors: Kempton James A. , Wynn Joe , Bond Sarah , Evry James , Fayet Annette L. , Gillies Natasha , Guilford Tim , Kavelaars Marwa , Juarez-Martinez Ignacio , Padget Oliver , Rutz Christian , Shoji Akiko , Syposz Martyna , Taylor Graham K. 
Published: March 2022

Dynamic soaring harvests energy from a spatiotemporal wind gradient, allowing albatrosses to glide over vast distances. However, its use is challenging to demonstrate empirically, and has yet to be confirmed in other seabirds. Here we investigate how flap-gliding Manx Shearwaters optimise their flight for dynamic soaring. We do so by deriving a new metric, the horizontal wind effectiveness, that quantifies how effectively flight harvests energy from a shear layer. We evaluate this metric empirically for fine-scale trajectories reconstructed from bird-borne video data using a simplified flight dynamics model. We find that the birds’ undulations are phased with their horizontal turning to optimise energy harvesting. We also assess the opportunity for energy harvesting in long-range, GPS-logged foraging trajectories, and find that Manx Shearwaters optimise their flight to increase the opportunity for dynamic soaring during favourable wind conditions. Our results show how small-scale dynamic soaring impacts large-scale Manx Shearwater distribution at sea.TeaserFlap-gliding shearwaters harvest wind energy by fine-scale trajectory optimization and this impacts their large-scale distribution at sea.
Intrinsic Protein Disorder, Conditional Folding and AlphaFold2
Authors: Piovesan Damiano , Monzon Alexander Miguel , Tosatto Silvio C.E. 
Published: March 2022

AbstractIntrinsically disordered regions (IDRs) defying the traditional protein structure-function paradigm have been difficult to analyze. AlphaFold’s recent breakthrough in predicting protein structures accurately offers a fresh perspective on IDR prediction as assessed on the CAID dataset. Surprisingly, AlphaFold is highly competitive for predicting both IDRs and conditionally folded regions, demonstrating the plasticity of the disorder to structure continuum.
One-pot synthesis of prenylated proteins utilizing E. coli cell-free expression
Authors: Kai Lei , Sonal  , Heermann Tamara , Schwille Petra 
Published: March 2022

AbstractBottom-up synthetic biology is a powerful tool for uncovering the mechanisms underlying vital biological processes, such as signaling and cell polarization. The core principle of reconstituting cellular functions in their minimal forms can be achieved through modular protein design. However, assembling multiple purified proteins into a functional and synchronized system remains a technical challenge. The fact that many regulatory proteins show direct or indirect membrane interactions further exacerbates the complications. Here, we introduce the Cell-Free prenylated Protein Synthesis (CFpPS) system which enables the production of prenylated proteins in a single reaction mix, through reconstituted prenylation machinery. Not only does the CFpPS system offer a fast and reliable method for producing solubilized prenylated proteins, but it can also produce the protein of interest directly in the vicinity of biomimetic membranes, thus enabling microscopy-based functional assessment. As proof of principle, we demonstrate synthesis and solubilization of various important signaling proteins from the Ras superfamily, as well as membrane binding and extraction of the key polarity regulator Cdc42. Furthermore, our method can be used to confer membrane affinity to any protein, simply by adding a 4-peptide motif to the C-terminus of the protein. In sum, the CFpPS system offers a versatile and effective platform for designing peripheral membrane proteins for synthetic biology applications.
The molecular chaperone Cysteine String Protein is required to stabilize trans-SNARE complexes during human sperm acrosomal exocytosis
Authors: Montero Karina Flores , Berberián María Victoria , Mayorga Luis Segundo , Tomes Claudia Nora , Ruete María Celeste 
Published: March 2022

AbstractMembrane fusion in sperm cells is crucial for acrosomal exocytosis and must be preserved to assure fertilizing capacity. Evolutionarily conserved protein machinery regulates acrosomal exocytosis. Molecular chaperones play a vital role in spermatogenesis and post-testicular maturation. Cysteine String Protein (CSP) is a member of the Hsp40 co-chaperones, and for more than 20 years, most research published focused on CSP’s role in synapsis. However, the participation of molecular chaperones in acrosomal exocytosis is poorly understood. Using western blot and indirect immunofluorescence, we showed that CSP is present in human sperm, is predominantly bound to membranes, and is palmitoylated. Moreover, using electron microscopy and functional assays, we reported that sequestration of CSP avoided the assembly of trans-complexes and inhibited exocytosis. In summary, our data demonstrated that CSP is necessary and mediates the trans-SNARE complex assembly between the outer acrosomal and plasma membranes, thereby regulating human sperm acrosomal exocytosis. Understanding CSP’s role is critical in identifying new biomarkers and generating new rational-based approaches to treating male infertility.Summary statementCysteine String Protein is necessary and mediates the trans-SNARE complex assembly between the outer acrosomal and plasma membranes, thereby regulating human sperm acrosomal exocytosis.
Helminth parasites decrease survival probability in young red deer
Authors: Acerini Claudia I. , Morris Sean , Morris Alison , Kenyon Fiona , McBean David , Pemberton Josephine M. , Albery Gregory F. 
Published: March 2022

AbstractHelminths are common parasites of wild ungulates that can have substantial costs for growth, mortality, and reproduction. While these costs are relatively well documented for mature animals, knowledge of helminths’ impacts on juveniles is more limited. Identifying these effects is important because young individuals are often heavily infected, and juvenile mortality is an important process regulating wild populations. Here, we investigated associations between helminth infection and overwinter survival in juvenile wild red deer (Cervus elaphus) on the Isle of Rum, Scotland. We collected faecal samples non-invasively from known individuals and used them to count propagules of three helminth taxa (strongyle nematodes,Fasciola hepatica, andElaphostrongylus cervi). Using generalised linear models, we investigated associations between parasite counts and overwinter survival for calves and yearlings. Strongyles were associated with reduced survival in both age class, andF. hepaticawas associated with reduced survival in yearlings, whileE. cerviinfection showed no association with survival in either age class. This study provides observational evidence for fitness costs of helminth infection in juveniles of a wild mammal, and suggests that these parasites could play a role in regulating population dynamics.
Employee treatment and discontinued operations
Authors: Leonard Lori , Sun Li 
Published: March 2022

PurposeThe authors investigate the relation between employee treatment and the likelihood of discontinuing business operations.Design/methodology/approachThe authors use regression analysis to investigate the relation between employee treatment and the likelihood of discontinuing business operations.FindingsThe authors find a significant negative relation between employee treatment and the likelihood of discontinuing business operations, suggesting that firms with better employee treatment are less likely to discontinue operations.Originality/valueThis study contributes to two distinctive steams of research: discontinued operations in accounting literature and employee welfare in human resources management literature.
Families_Share: digital and social innovation for work–life balance
Authors: Cortesi Agostino , Berionni Carlotta , Veeckman Carina , Leonardi Chiara , Schiavo Gianluca , Zancanaro Massimo , Cescon Marzia , Sangiuliano Maria , Tampakis Dimitris , Falelakis Manolis 
Published: March 2022

Purpose
The European H2020 Families_Share project aims at offering a grass-root approach and a co-designed platform supporting families for sharing time and tasks related to childcare, parenting, after-school and leisure activities and other household tasks. To achieve this objective, the Families_Share project has been built on current practices which are already leveraging on mutual help and support among families, such as Time Banks, Social Streets and self-organizing networks of parents active at the neighbourhood level and seek to harness the potential of ICT networks and mobile technologies to increase the effectiveness of participatory innovation. The aim of this paper is to present and discuss the Families_Share methodology and platform, as well as the results obtained by several partecipating communities in different European countries.

Design/methodology/approach
This paper discusses how the Families Share approach (CAPS project, Horizon 2020) is bringing the sharing economy to childcare. Families Share developed a co-caring approach and a co-designed digital welfare platform to support parents with sharing time and tasks related to childcare, after-school and leisure activities. Families Share conducted two iterative pilot experiments and related socio-economic evaluations in six European cities. More than 3,000 citizens were engaged in the co-design process through their local community organizations and more than 1,700 parents and children actively experimented with the approach by organizing collaborative childcare activities. The authors discuss the challenges and solutions of co-designing a socio-technical approach aimed at facilitating socially innovative childcare models, and how the Families Share approach, based on technology-supported co-production of childcare, may provide a new sustainable welfare model for municipalities and companies with respect to life––work balance.

Findings
The authors discuss the challenges and solutions of co-designing a technological tool aimed at facilitating socially innovative childcare models, and how the Families Share approach may provide a new sustainable welfare model for municipalities and companies with respect to work–life balance.

Originality/value
As a main difference with state-of-the-art proposals, Families_Share is aimed to provide support to networks of parents in the organization of self-managed activities, this way being orthogonal with respect either to social-network functionalities or to supply and demand services. Furthermore, Families_Share has been based on a participative approach for both the ICT platform and the overall structure.
Residential occupants’ service quality in mixed developments
Authors: Olanrewaju AbdulLateef , Shia Phang Horng , Chu Hui Chen 
Published: March 2022

PurposeThe demand for residential units in mixed development is increasing because of a better understanding of the benefits and functions of mixed development. However, there is a lack of study on the performance of the residential buildings in the mixed development. The purpose of this study is to investigate the satisfaction level of the occupants of residential buildings in mixed developments.Design/methodology/approachThe research developed a questionnaire instrument that included 17 factors to measure the expectations and actual satisfaction of the residences in the mixed development. Data were collected using a survey questionnaire from five mixed developments in Greater Kuala Lumpur and analysed with service quality and matrix chart models.FindingsThe data revealed that while the expectations of the residential building users in mixed developments were not excessive, their satisfaction levels were not high. The price of residential buildings in mixed developments is exorbitant. Many of the occupants have relatively high actual satisfaction, even though some of them are disappointed in some of the services or benefits that are expected in mixed developments. The occupants are not interested in the nearness of the mixed development to public transport facilities, suggesting a high urge for private car ownership and poor public transport facilities.Originality/valueThe findings will be useful to developers, design teams, city planners and policymakers. Implicit in the findings of this research is the lack of a benchmark for the performance of luxury housing. From an ontological standpoint, the main findings of this study are generalisable to other cities in and outside of Malaysia.
Linking workplace ostracism and knowledge hoarding via organizational climate: a review and research agenda
Authors: Dash Debasis , Farooq Rayees , Upadhyay Satwik 
Published: March 2022

Purpose
This study aims to analyze the relationship between workplace ostracism and knowledge hoarding. The study also proposes a mediational role of organizational climate in the relationship between workplace ostracism and knowledge hoarding.

Design/methodology/approach
The procedure used in the study is a systematic literature review covering workplace ostracism, knowledge hoarding and organizational climate from 1986 to 2021. The studies were explored using keyword searches such as “Workplace ostracism”, “Knowledge hoarding” and “Organizational climate” from the selected databases, namely, Scopus, Web of Science and Google Scholar.

Findings
The systematic review of 146 articles found most studies suggesting that workplace ostracism affects employees’ personal and professional relationships and is a precursor to knowledge hoarding behaviours. The workplace ostracism–knowledge hoarding relationship has a logical explanation as knowledge hoarding is often perceived as counter-normative, which can reduce the performance of a hoarder, leading to behaviours such as ostracism and expulsion. The review proposes that organizational climate plays a significant role in improving the workplace ostracism and knowledge hoarding relationship. The study introduces some fundamental propositions towards the development of a model for future research.

Originality/value
The study attempts to uncover a series of relationships between workplace ostracism, knowledge hoarding and organizational climate, which may enhance academic discussion and offer clarity to the conceptualization of these two fields.
Integration of Six Sigma methodology of DMADV steps with QFD, DFMEA and TRIZ applications for image-based automated inspection system development: a case study
Authors: Purushothaman Kumaraendran , Ahmad Rosmaini 
Published: March 2022

Purpose
This paper aims to present the development of an automated inspection system (AIS) using an image-based analysis mechanism, called i-AIS. The development process of i-AIS used the Design Six Sigma (DSS) methodology. The steps of define, measure, analyze, design and verify (DMADV) are applied and integrated with specific analyses techniques of the quality function deployment (QFD), design failure mode effect analysis (DFMEA) and theory of inventive problem solving (TRIZ). The production process of adhesive tape is the focused case study in this research project, motivated by the high product defect rate complained by customers.

Design/methodology/approach
The development process of i-AIS was divided into five standard steps based on the DSS methodologies of DMADV. One of the key processes in this development was to systematically identify the right and intended features of i-AIS. This was carried out based on the application of the QFD technique. Another important process was to further investigate the possible causes of i-AIS failure, to function as intended. This investigative process was carried out based on the DFMEA technique, while the solution to minimize the risk of the identified failures was obtained from the TRIZ method. The final prototype of i-AIS was then presented in the design step.

Findings
Verification of the i-AIS prototype revealed its operation at an optimally intended mode that fulfilled the requirements of internal customers. Verification results also revealed that the sigma level has improved from 3.87 to 4.33. Meanwhile, the defect reduction rate is improved to 74.4% and downtime rate also recorded a significant improvement at 80.7% of reduction.

Research limitations/implications
The presented research work is carried out based on a customized case study. Although the proposed methodology can be applied to others cases towards design-based solution, some modifications maybe required based on to the unique features of the case study under consideration.

Practical implications
The presented research project indicated that the proposed methodology was successful to facilitate a structured and systematic process towards defect identification, classification, evaluation and generation of a solution.

Originality/value
The paper presents the development process of an AIS by considering comprehensive managerial aspects that are currently absent in the literature. An integrated DSS structure is proposed to systematically guide the development of i-AIS. The related managerial aspects such as identification of critical defects problem, customer requirement mapping, prototype design analysis and comparison measurements before and after i-AIS installation are considered in this research project.
Celebrating 15 years of the JAOC: a critical overview and reflections
Authors: Moll Jodie 
Published: March 2022

Purpose
The JAOC has reached an important milestone. It is now 15 years old! This study aims to provide an understanding of the key achievements of the Journal.

Design/methodology/approach
This paper reports the findings of a bibliometric analysis of the Journal.

Findings
The Journal has made significant contributions towards understanding the various purposes or roles of accounting and the context within which it operates in diverse societies. Case studies in the Third Sector and in countries that tend to be unrepresented in many other journals, such as Laos and Vietnam, represent some of the critical contributions of papers published in the JAOC.

Originality/value
This paper provides a review of the Journal. Key achievements are noted along with trends in the usage of theories and topics. Gaps between the aims of the Journal and what it has achieved are also outlined.