bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sexual intimacy and aging: An integrative framework to promote intimacy resilience in couple therapy
Authors: Scheinkman Michele , Iasenza Suzanne , Ludwig Keren , Cronin Thomas , Lemor Susan , Papp Peggy 
Published: March 2022

AbstractIn our work at the Intimacies Project at The Ackerman Institute for the Family we became aware of a gap in attention about sexuality and aging in the couple and family therapy field. In this article, we provide an integrative framework to guide therapists on how to address problems of sexuality and aging in the therapy room. Starting from considerations about the social context of aging and the self of the therapist, we contend that when normative sexual challenges become entangled with stigma, misconceptions about sexuality, limiting gender narratives, vulnerabilities, and defensive postures, they often result in emotional and sexual shutdowns. Through a combination of the vulnerability cycle with an expansive definition of sexuality, we demonstrate how we deconstruct impasses, disentangle normative quandaries from reactive dynamics, and help couples transform their sexual narratives. We outline how we conduct individual sessions to obtain relational sexual histories, utilize Sensate Focus as a mindful touch exercise, and help partners expand their sexual menus beyond penetration and orgasms. We also describe relational skills that may need to be strengthened to help aging couples deal with the ebb and flow of intimacy, sustaining resilience over time.
Association between sodium‐glucose co‐transporter 2 inhibitors and risk of bullous pemphigoid in patients with type 2 diabetes: a nationwide population‐based cohort study
Authors: Ma S.H. , Wu C.Y. , Lyu Y.S. , Chou Y.J. , Chang Y.T. , Wu C.Y. 
Published: March 2022

AbstractBackgroundCertain anti‐diabetic agents have been linked to the development of bullous pemphigoid (BP). However, the relationship between BP and sodium‐glucose co‐transporter 2 inhibitors (SGLT2is) remains inconclusive.ObjectiveTo investigate the association between SGLT2i usage and BP.MethodsParticipants were recruited from the Taiwan National Health Insurance Database between 2007 and 2018. A total of 149 060 patients with diabetes receiving SGLT2i were matched 1 : 2 with diabetic patients without SGLT2i usage. Factors such as age, sex, duration of diabetes condition, DPP4i usage, insulin usage and selected comorbidities were included in the multivariate analysis.ResultsCompared with the control, the 2‐year‐cumulative incidence was significantly low in patients using SGLT2i after adjustment for competing mortality. Patients with diabetes receiving SGLT2i had a low risk [adjusted hazard ratio (HR) 0.56, 95% confidence interval (CI), 0.33–0.96] for BP after adjustment for potential confounders. Age (HR, 1.06), renal disease (HR, 1.79), cerebrovascular disease (HR, 3.23), epilepsy (HR, 3.07), DPP4i users (HR: 2.55) and insulin users (HR: 2.56) were significant risk factors for BP.ConclusionsThe risk of BP did not increase in patients receiving SGLT2i. Thus, SGLT2i could be a safe choice for patients with diabetes having additional risk factors or a history of BP.
An update on the evolutionary origin of aglomerular kidney with structural and ultrastructural descriptions of the kidney in three fish species
Authors: Senarat Sinlapachai , Kettratad Jes , Pairohakul Supanut , Ampawong Sumate , Huggins Brian P. , Coleman Melissa M. , Kaneko Gen 
Published: March 2022

AbstractThe kidney of fish contains numerous nephrons, each of which is divided into the renal corpuscle and renal tubules. This glomerular structure is the filtration unit of the nephron and is important for the kidney function, but it has been reported that the renal corpuscle was lost in at least four independent linages of fish (i.e., aglomerular kidney). In this study, the authors newly described renal structures for three species by histological and ultrastructural observations: two aglomerular kidneys from a seahorse Hippocampus barbouri and a toadfish Allenbatrachus grunniens and a glomerular kidney from a snake eel Pisodonophis boro. The renal development of H. barbouri was also described during 1–35 days after birth. In all species tested, the anterior kidney was comprised of haematopoietic tissues and a few renal tubules, whereas the posterior kidney contained more renal tubules. Although the glomerular structure was present in P. boro, light microscopic observations identified no glomeruli in the kidney of H. barbouri and A. grunniens. Ultrastructurally, abundant deep basal infoldings with mitochondria in the renal tubules were observed in A. grunniens compared to H. barbouri and P. boro, suggesting the possible role of basal infoldings in maintaining the osmotic balance. By integrating the results from the three species and comprehensive literature search, the authors further showed that 56 species have been reported to be aglomerular, and that the aglomerular kidney has evolved at least eight times in bony fishes.
Nurse species facilitate persistence of dry forests in agricultural landscapes in Uruguay
Authors: Pozo Inofuentes Paola , Säumel Ina 
Published: March 2022

AbstractQuestionsPark forests are shaped by extensive cattle ranching in the transition between natural riverine forests and open grasslands. The underlying mechanisms driving tree regeneration have not been studied, however, they determine biodiversity, sustainability and multifunctionality of these ecosystems. We explored patterns of tree regeneration by analyzing nurse–beneficiary interactions and tree community composition.LocationPark forests within the departments of Rio Negro, Paysandú, Artigas and Tacuarembó, República Oriental del Uruguay, South America.MethodsWe established 205 (1 × 1 m2) paired plots in open microhabitat and under the tree canopy to evaluate the patterns of tree regeneration. To assess tree community composition and diversity we carried out forest inventories within one‐hectare plots of ten park forests.ResultsScattered trees in park forests had a positive effect on tree regeneration density, whereas, dense grass coverage had a negative effect. Regeneration density increased and grass cover reduced under the canopy of nurse trees. Regeneration beneficiaries were mainly bird dispersed species with different life strategies. Tree communities varied between forest types and spatially closer forests were more similar.ConclusionsOur study outlines the importance of park forest trees to the promotion of forest regeneration and recovery in grazed forests. Our results found a positive interaction effect between nurse trees and saplings, based on our results we suggested possible facilitation mechanisms including the provision of shade, reduction of grass competition and the attraction of seed dispersers.
Quality of life in liver transplant recipients during the Corona virus disease 19 pandemic: A multicentre study
Authors: Gitto Stefano , Golfieri Lucia , Mannelli Nicolò , Tamè Maria Rosa , Lopez Ivo , Ceccato Roberta , Montanari Stanislao , Falcini Margherita , Vitale Giovanni , De Maria Nicola , Presti Danilo Lo , Marzi Luca , Mega Andrea , Valente Giovanna , Borghi Alberto , Foschi Francesco Giuseppe , Grandi Silvana , Forte Paolo , Cescon Matteo , Di Benedetto Fabrizio , Andreone Pietro , Arcangeli Giulio , De Simone Paolo , Bonacchi Andrea , Sofi Francesco , Morelli Maria Cristina , Petranelli Marco , Lau Chloe , Marra Fabio , Chiesi Francesca ,   for the MEDITRA Group:, Vizzutti Francesco , Vero Vittoria , Di Donato Roberto , Berardi Sonia , Pianta Paolo , D’Anzi Sharon , Schepis Filippo , Gualandi Noemi , Miceli Francesca , Villa Erica , Piai Guido , Valente Marco , Campani Claudia , Lynch Erica , Magistri Paolo , Cursaro Carmela , Chiarelli Annarita , Carrai Paola , Petruccelli Stefania , Dinu Monica , Pagliai Giuditta 
Published: March 2022

AbstractBackgroundLiver transplant recipients require specific clinical and psychosocial attention given their frailty. Main aim of the study was to assess the quality of life after liver transplant during the current pandemic.MethodsThis multicentre study was conducted in clinically stable, liver transplanted patients. Enrollment opened in June and finished in September 2021. Patients completed a survey including lifestyle data, quality of life (Short Form health survey), sport, employment, diet. To examine the correlations, we calculated Pearson coefficients while to compare subgroups, independent samples t‐tests and ANOVAs. To detect the predictors of impaired quality of life, we used multivariable logistic regression analysis.ResultsWe analysed data from 511 patients observing significant associations between quality of life’s physical score and both age and adherence to Mediterranean diet (p < .01). A significant negative correlation was observed between mental score and the sedentary activity (p < .05). Female patients scored significantly lower than males in physical and mental score. At multivariate analysis, females were 1.65 times more likely to report impaired physical score than males. Occupation and physical activity presented significant positive relation with quality of life. Adherence to Mediterranean diet was another relevant predictor. Regarding mental score, female patients were 1.78 times more likely to show impaired mental score in comparison with males. Sedentary activity and adherence to Mediterranean diet were further noteworthy predictors.ConclusionsFemales and subjects with sedentary lifestyle or work inactive seem to show the worst quality of life and both physical activity and Mediterranean diet might be helpful to improve it.
Patterns of belowground overyielding and fine‐root biomass in native and exotic angiosperms and gymnosperms
Authors: Schuster Michael J. , Williams Laura J. , Stefanski Artur , Bermudez Raimundo , Belluau Michaël , Messier Christian , Paquette Alain , Gravel Dominique , Reich Peter B. 
Published: March 2022

Mixing tree species can lead to more productive forests, but how belowground productivity is affected by mixtures of trees of diverse phylogenetic and eco‐evolutionary histories is unclear. Here, we examine how species origin and phylogeny affect belowground productivity in tree communities of varied richness and functional diversity. We measured standing fine‐root biomass and annual fine‐root production across 41 assemblages of 12 tree species, representing both angiosperms and gymnosperms originating from North America and Europe. Increasing functional diversity of mixtures stimulated overyielding of annual production but did not affect standing biomass. In general, annual productivity of mixtures of species that were less productive in monoculture had neutral (angiosperms) to positive (North American species: +16%) responses to mixing, whereas annual productivity of mixtures of species that were more productive in monoculture had neutral (European species) to negative (gymnosperms: −6%) responses to mixing. These differences translated into angiosperm mixtures overyielding in standing biomass by 16% but no effects of mixing on gymnosperm mixtures. The trends we observed between North American and European species annual production were reversed when considering standing biomass. European mixtures had 14% more standing biomass and North American mixtures had 10% less standing biomass than expected from monocultures.Our study offers a rare examination of the combined roles of origin and phylogeny in forest fine‐root productivity, and suggests varied consequences of biodiversity change for forest belowground productivity based on composition. In North America, belowground productivity of young forests composed of angiosperms and native tree species may be more tightly linked to diversity than that of forests dominated by gymnosperms or European species. This suggests that increased diversity may lead to the greatest enhancement of belowground productivity in native, North American forests dominated by angiosperms, but also that declines in diversity may be felt most strongly in these forests as well.
The right to a healthy environment and its justiciability before the Inter‐American Court of Human Rights: A critical appraisal of the Lhaka Honhat v Argentina judgement
Authors: Mejía‐Lemos Diego 
Published: March 2022

AbstractThe Inter‐American Court of Human Rights has recently changed its approach to the protection of environmental rights, which are now treated as autonomously and directly justiciable, thus without having to first establish a breach of any related civil or political rights. Lhaka Honhat v Argentina is the first contentious case the Court adjudicates based on the right to a healthy environment. The judgement issued in this case, and the opportunities and challenges presented by direct justiciability of environmental rights within the Inter‐American system, are critically appraised.
Outbreak of follicular conjunctivitis associated with the diagnosis of anaplasmosis in a sheep herd
Authors: Oliveira Paula M. , Ramos Dirceu G. S. , Saturnino Klaus C. , Amaral Andréia V. C. 
Published: March 2022

AbstractObjectiveTo report an outbreak of follicular conjunctivitis in a group of sheep diagnosed with Anaplasma spp., without any other co‐infection.Animals StudiedIn all, 18 animals from a sheep head, males and females, from eight months to four years of age, were assessed for follicular conjunctivitis.ProceduresThe procedures performed included general physical and ophthalmological examinations; PCR evaluation for infectious agents; analysis of hematological parameters, microbiological tests of ocular swabs, coproparasitological examination, histopathological examination of conjunctival biopsy.ResultsAll 18 animals had uni‐ or bilateral follicular conjunctivitis, and one animal also had unilateral uveitis. The results of microbiological analyzes were negative for Moraxella spp., Staphylococcus spp., and Pseudomonas spp., and PCR analysis results were negative for Chlamydia spp., Mycoplasma spp., and Toxoplasma gondii. Anemia, thrombocytopenia, lymphocytosis, and an inclusion body in some erythrocytes, compatible with Anaplasma and PCR analysis for Anaplasma spp. were positive.ConclusionAnaplasmosis may be associated with follicular conjunctivitis in sheep and should be included in the differential diagnosis list and investigated in cases of conjunctivitis in herds.
Loss of thymidine phosphorylase activity disrupts adipocyte differentiation and induces insulin-resistant lipoatrophic diabetes
Authors: Gautheron Jérémie , Lima Lara , Akinci Baris , Zammouri Jamila , Auclair Martine , Ucar Sema Kalkan , Ozen Samim , Altay Canan , Bax Bridget E. , Nemazanyy Ivan , Lenoir Véronique , Prip-Buus Carina , Acquaviva-Bourdain Cécile , Lascols Olivier , Fève Bruno , Vigouroux Corinne , Noel Esther , Jéru Isabelle 
Published: March 2022

Abstract
Background
Thymidine phosphorylase (TP), encoded by the TYMP gene, is a cytosolic enzyme essential for the nucleotide salvage pathway. TP catalyzes the phosphorylation of the deoxyribonucleosides, thymidine and 2′-deoxyuridine, to thymine and uracil. Biallelic TYMP variants are responsible for Mitochondrial NeuroGastroIntestinal Encephalomyopathy (MNGIE), an autosomal recessive disorder characterized in most patients by gastrointestinal and neurological symptoms, ultimately leading to death. Studies on the impact of TYMP variants in cellular systems with relevance to the organs affected in MNGIE are still scarce and the role of TP in adipose tissue remains unexplored.

Methods
Deep phenotyping was performed in three patients from two families carrying homozygous TYMP variants and presenting with lipoatrophic diabetes. The impact of the loss of TP expression was evaluated using a CRISPR-Cas9-mediated TP knockout (KO) strategy in human adipose stem cells (ASC), which can be differentiated into adipocytes in vitro. Protein expression profiles and cellular characteristics were investigated in this KO model.

Results
All patients had TYMP loss-of-function variants and first presented with generalized loss of adipose tissue and insulin-resistant diabetes. CRISPR-Cas9-mediated TP KO in ASC abolished adipocyte differentiation and decreased insulin response, consistent with the patients’ phenotype. This KO also induced major oxidative stress, altered mitochondrial functions, and promoted cellular senescence. This translational study identifies a new role of TP by demonstrating its key regulatory functions in adipose tissue.

Conclusions
The implication of TP variants in atypical forms of monogenic diabetes shows that genetic diagnosis of lipodystrophic syndromes should include TYMP analysis. The fact that TP is crucial for adipocyte differentiation and function through the control of mitochondrial homeostasis highlights the importance of mitochondria in adipose tissue biology.
An unexpected case of Borrelia garinii liver infection
Authors: Duffau Pierre , Korbi Skander , Guillotin Vivien , Talagrand-Reboul Emilie , Ménard Armelle , Peuchant Olivia 
Published: March 2022

Abstract
Background
Lyme borreliosis is the most prevalent arthropod-borne infection in the Northern Hemisphere. In Europe, Borrelia afzelii is predominantly involved in cutaneous manifestations, Borrelia garinii and Borrelia bavariensis in neurological manifestations, and Borrelia burgdorferi sensu stricto in articular ones. Liver impairement is not classical in Lyme borreliosis. Diagnosis is currently mainly based on serological testing, and is challenging in immunocompromised patients.

Case presentation
We report the first case of B. garinii infection revealed by liver involvement in an immunocompromised man. A 73-year-old man with marginal zone lymphoma, treated with bendamustine and rituximab, developed intermittent fever and inflammatory syndrome. Microbial investigations were all negative and FDG-PET showed complete remission of the lymphoma. Three months later, liver biopsy was performed and histology revealed spirochetes-like bacteria. Microbial diagnosis was performed by 16S rDNA sequencing, flagellin (flaB) gene sequencing and multi-locus sequence typing and identified B. garinii. The patient recovered successfully after a three weeks course of antibiotics. Diagnosis was challenging because Borrelia hepatic involvement is unusual and no erythema migrans nor tick bite were notified.

Conclusion
This case highlights that unexplained fever and inflammatory syndrome in immunocompromised patients warrants specific investigations to identify bacteria such as spirochetes.
Feasibility of the wingate anaerobic exercise test as a clinical measure in patients with juvenile dermatomyositis
Authors: Dover Saunya , Stephens Samantha , Clairman Hayyah , Abesamis Andrew , Jazi Omidali Aghababaei , Babij Stephanie , Marcuz Jo-Anne , Naraidoo Natasha , Pan Jing , Pullenayegum Eleanor , Rumsey Dax , Whitney Kristi , Feldman Brian M. 
Published: March 2022

Abstract
Background
Core sets, while widely adopted for clinical assessment in juvenile dermatomyositis (JDM), have some drawbacks – they are time consuming, were developed primarily for research, and require an experienced multidisciplinary team. We propose the Wingate Anaerobic Test, a 30-s all out test performed on a cycle ergometer, as a potential alternative; it is valid and reliable in this patient population. We aimed to determine the feasibility of performing the Wingate test as part of a typical clinic visit, and to determine if it is correlated to current measures of disease activity.

Methods
Patients 5–18 years of age, with JDM, were recruited from the JDM clinic at a large Canadian academic children’s hospital. Participants underwent a standard clinic assessment, then completed a Wingate test at the end of the visit.

Results
Twenty-six patients participated in the study, representing a recruitment rate of 81%; of those, 88% were able to complete the Wingate test. Patients liked the Wingate test and felt it should be included as a regular clinic test. Absolute peak power (watts) on the Wingate test was strongly correlated to the manual muscle test (MMT-8) and the timed squat test. Relative peak power (watts/kg) on the Wingate test was strongly correlated to the timed squat test and the Childhood Myositis Assessment Scale (CMAS). Exploratory principal components analysis revealed that Wingate relative average power explained almost 2/3 of the variance of the CMAS, MMT and timed squats combined.

Conclusion
The Wingate test is a feasible test for children with JDM and correlates well with standard clinical assessments. Given its brevity, it has the potential to replace more standard measures of physical function currently used in clinical assessments for children with JDM. Future work should focus on how best to operationalize Wingate testing in clinic without the use of dedicated personnel.
Electroencephalographic findings after convulsive seizures due to cerebral arterial air embolism secondary to lung cancer: a case report
Authors: Inatomi Shinichiro , Izumi Tesseki , Eura Nobuyuki , Sato Ichiro , Tasaki Masato , Muro Shigeo , Sugie Kazuma 
Published: March 2022

Abstract
Background
Cerebral arterial air embolism is often associated with an invasive iatrogenic etiology and a high rate of convulsive seizures. There are only a few descriptions of electroencephalogram findings in convulsive seizures due to cerebral arterial air embolism of noniatrogenic etiology. Herein, we describe the case of a patient with lung cancer and convulsive seizures with abnormalities detected on electroencephalogram caused by cerebral arterial air embolism of noniatrogenic etiology.

Case presentation
A 55-year-old Japanese man underwent radiotherapy and chemotherapy for cancer in the hilum of the left lung that was diagnosed after hemoptysis. One year after the diagnosis, he developed fever and chest pain that required hospitalization. At admission, he was in shock, and chest computed tomography revealed invasion of the left atrium and left main bronchus by the hilar cancer. Chest and abdominal computed tomography revealed small low-density areas within the tumor and around the intestinal membrane, which were interpreted as the presence of air due to invasion of the lung cancer. He was diagnosed with septic shock due to necrotic infection secondary to cancer invasion into the left atrium. The following day, he complained of difficulty in speaking and weakness in the left side of his body. A head computed tomography scan revealed multiple small low-density areas in the right cortex and bilateral subcortex, which were interpreted as air emboli. On day 3, he experienced generalized tonic–clonic seizures for approximately 1 minute, followed by myoclonus-like convulsions in the left lower limb and a right-sided gaze. The electroencephalogram findings after the convulsive seizures revealed partial epilepsy-like waves with intermittent spikes in the bilateral cerebral hemispheres and a diffuse slow wave in the left frontal lobe. He recovered from sepsis without recurrence of convulsive seizures; however, he died of hemoptysis on day 50 after discharge.

Conclusions
Electroencephalogram findings of focal spike activities and diffuse slow waves were detected in early seizures due to cerebral arterial air embolism of noniatrogenic etiology associated with lung cancer. Additional case descriptions are warranted to establish patterns in electroencephalogram findings specific to cerebral arterial air embolism.