bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS‐CoV‐2 whole‐proteome sequences from environment as an indicator of community viral distribution, evolution and epidemiological dynamics: A cohort analysis of Austria
Authors: Magateshvaren Saras Murali Aadhitya , Patro L. Ponoop Prasad , Uttamrao Patil Pranita , Rathinavelan Thenmalarchelvi 
Published: June 2022

AbstractSeveral investigations have been carried out to detect SARS‐CoV‐2 samples from the environment such as sewage waters and surface swabs. Whole‐proteome sequence analysis of 847 SARS‐CoV‐2 genome sequences collected from the environment in Austria during 2021 and deposited in GISAID indicates that alpha and delta are two dominant variants, coinciding with the human clinical samples with a Pearson correlation coefficient in the range of 0.58 (alpha variant) to 0.82 (delta variant). Both environmental and human samples show that Austrian SARS‐CoV‐2 alpha variant is found to possess N protein R203K and G204R/P mutations, whereas they are absent in the delta variant. SARS‐CoV‐2 delta variant is continuously seen in both the environmental and human clinical samples from the month of September 2021 and it spiked in November 2021, which is directly reflected in the increase of the number of SARS‐CoV‐2 infections and deaths in Austria during November 2021. Thus, the results presented here indicate that the environmental SARS‐CoV‐2 whole‐genome sequences collected from Austria reflect the community viral distribution, evolution and the concomitant epidemiological dynamics. Since SARS‐CoV‐2 keeps evolving, the results presented here further suggest the need to monitor the environment for the early detection of SARS‐CoV‐2 variants to take appropriate precautionary measures.
How Accounting Ends: Self‐Undermining Repetition in Accounting Life Cycles*
Authors: Palermo Tommaso , Power Michael , Ashby Simon 
Published: June 2022

ABSTRACTThis study develops a process model of how accounting may come to an end. Grounded in a longitudinal study of a risk culture survey, this model focuses on the dynamics that underpin the repetition of accounting practices, and sheds light on two boundary conditions of successful repetition and continuation, which are in tension with each other. On the one hand, there are pressures for repetition that preserves continuity and comparability. On the other hand, there is the ongoing organizational need to adjust accounting practices. Iterating between the case study findings, social studies of accounting, and the sociology of replication in scientific practice, the model shows how moving too close to either boundary increases the risk that repetition undermines the accounting practice being repeated: “perfect repetition” may be perceived as uninteresting and decision‐irrelevant; very “imperfect repetition” may be perceived as something too different and idiosyncratic, and hence also decision‐irrelevant. As a result, the analysis extends a rich literature that has examined empirical instances of failure of the conditions that sustain the repeatability of accounting practices. Via the theory of “self‐undermining repetition,” this study shows how the possibilities for accounting's ending are paradoxically inherent in the very act of repetition. This notion of “self‐undermining repetition” is deepened by a discussion of how it may be affected by four contingencies: task ambiguity, organizational politics, organizational actors' reflexivity, and external networks of support. Overall, the analysis of the self‐undermining dynamics of repetition and related contingencies contrasts with research that foregrounds the constitutive nature of repeated uses of accountings. It shows how repetition may also undermine, rather than cumulatively consolidate, accounting practices.
Foliar application of wood distillate boosts plant yield and nutritional parameters of chickpea
Authors: Fedeli Riccardo , Vannini Andrea , Celletti Silvia , Maresca Viviana , Munzi Silvana , Cruz Cristina , Alexandrov Dmitry , Guarnieri Massimo , Loppi Stefano 
Published: June 2022

AbstractIn the quest for eco‐friendly products with biostimulant properties, foliar application of wood distillate (WD) was tested on the growth and yield of chickpea (Cicer arietinum L.). WD (pyroligneous acid) is a by‐product of plant biomass pyrolysis and is rich in biologically active substances like polyphenols, alcohols, acids and esters. In this work, chickpea plants were sprayed weekly with 100 ml 0.25% (v/v) chestnut (Castanea sativa Mill.) WD during the whole growing period, and at the end physiological and nutritional analyses were performed both on the whole plant and on seeds. While plant height and weight did not change significantly, seeds showed an increase in diameter (+11.2%) and weight (+33.3%), and in the content of starch (+45.9%), total soluble protein (+12.9%), total polyphenol (+16.4%) and antioxidant power (+28.4%). Overall, the content of essential free amino acids increased, except for lysine (−3.4%), phenylalanine (−10.5%) and methionine (−13.7%). Among all the mineral elements analysed, only potassium and magnesium decreased in WD‐treated plants, although values were within the common range for chickpea seeds. These results are a clear demonstration of the effectiveness of the use of WD on increasing the nutritional qualities of the edible parts of crop species, thus representing a possible solution to counteract human malnutrition and famine as well as environmental concerns.
Medication prescribing for alcohol use disorders during alcohol‐related encounters in a Colorado regional healthcare system
Authors: Chockalingam Leela , Burnham Ellen L. , Jolley Sarah E. 
Published: June 2022

AbstractRationaleInvestigations show that medications for alcohol use disorders (MAUD) reduce heavy drinking and relapses. However, only 1.6% of individuals with alcohol use disorders (AUD) receive MAUD across care settings. The epidemiology of MAUD prescribing in the acute care setting is incompletely described. We hypothesized that MAUD would be under prescribed in inpatient acute care hospital settings compared to the outpatient, emergency department (ED), and inpatient substance use treatment settings.MethodsWe evaluated electronic health record (EHR) data from adult patients with an International Classification of Diseases, 10th revision (ICD‐10) alcohol‐related diagnosis in the University of Colorado Health (UCHealth) system between January 1, 2016 and 31 December, 2019. Data from patients with an ICD‐10 diagnosis code for opioid use disorder and those receiving MAUD prior to their first alcohol‐related episode were excluded. The primary outcome was prescribing of MAUD, defined by prescription of naltrexone, acamprosate, and/or disulfiram. We performed bivariate and multivariate analyses to identify independent predictors of MAUD prescribing at UCHealth.ResultsWe identified 48,421 unique patients with 136,205 alcohol‐related encounters at UCHealth. Encounters occurred in the ED (42%), inpatient acute care (17%), inpatient substance use treatment (18%), or outpatient primary care (12%) settings. Only 2270 (5%) patients received MAUD across all settings. Female sex and addiction medicine consults positively predicted MAUD prescribing. In contrast, encounters outside inpatient substance use treatment, Hispanic ethnicity, and black or non‐white race were negative predictors of MAUD prescribing. Compared to inpatient substance use treatment, inpatient acute care hospitalizations for AUD was associated with a 93% reduced odds of receiving MAUD.ConclusionsAUD‐related ED and inpatient acute care hospital encounters in our healthcare system were common. Nevertheless, prescriptions for MAUD were infrequent in this population, particularly in inpatient settings. Our findings suggest that the initiation of MAUD for patients with alcohol‐related diagnoses in acute care settings deserves additional evaluation.
Correlates of high phosphatidylethanol (PEth) levels and their concordance with self‐reported heavy alcohol consumption among men who have sex with men who binge drink alcohol
Authors: McLaughlin Matthew F. , Jain Jennifer P. , Ikeda Janet , Walker John E. , Coffin Phillip , Santos Glenn‐Milo 
Published: June 2022

AbstractBackgroundHeavy alcohol use, including binge drinking, is associated with high morbidity and mortality among men who have sex with men (MSM). Self‐reported alcohol measures may lead to inaccurate estimates due to recall and social desirability biases. Objective alcohol biomarkers like phosphatidylethanol (PEth) can be used to corroborate self‐report and could help to inform treatment approaches and research strategies for alcohol using MSM.MethodsFrom 2015 to 2020, alcohol using MSM ≥18 years were enrolled in a randomized controlled trial evaluating the efficacy of naltrexone in reducing binge drinking. Using this trial's baseline data, we applied multivariable logistic regression to identify the correlates of high PEth levels (i.e., ≥87 ng/ml) and concordance between PEth levels and self‐reported heavy drinking.ResultsOf 118 MSM, 64% had PEth levels ≥87 ng/ml and 72% had PEth levels that were concordant with self‐reported heavy alcohol use. Factors significantly associated in separate models with elevated PEth levels were income ≥$60,000 (adjusted odds ratio [aOR] = 4.09; 95% CI = 1.13 to 14.82), being employed (aOR = 4.04; 95% CI = 1.45 to 11.32), episodic cannabis use (aOR = 4.63; 95% CI = 1.27 to 16.92), and any alcohol/substance use prior to or during anal intercourse (aOR = 2.52; 95% CI = 1.08 to 5.90). Living with HIV was associated with significantly lower odds of elevated PEth levels (aOR = 0.23; 95% CI = 0.09 to 0.61). Factors associated with significantly higher concordance between PEth levels and self‐reported heavy alcohol use included at least weekly use of poppers (aOR = 6.41; 95% CI = 1.27 to 32.28) and polysubstance use (aOR = 2.53; 95% CI = 1.02 to 6.27). Living with HIV was associated with lower odds of concordance (aOR = 0.36; 95% CI = 0.14 to 0.97).ConclusionsPEth may enhance the detection of heavy drinking among MSM, including the identification of subpopulations that may benefit from targeted alcohol reduction interventions. However, PEth values for MSM living with HIV showed modest concordance with self‐reported alcohol use and may need to be supplemented with additional biomarkers or evaluated against a different cutoff.
Mice lacking α4 nicotinic acetylcholine receptors are protected against alcohol‐associated liver injury
Authors: Watson Walter H. , Ritzenthaler Jeffrey D. , Torres‐Gonzalez Edilson , Arteel Gavin E. , Roman Jesse 
Published: June 2022

AbstractBackgroundChronic heavy alcohol consumption is a major risk factor for the development of liver steatosis, fibrosis, and cirrhosis, but the mechanisms by which alcohol causes liver damage remain incompletely elucidated. This group has reported that α4 nicotinic acetylcholine receptors (α4 nAChRs) act as sensors for alcohol in lung cells. This study tested the hypothesis that α4 nAChRs mediate the effects of alcohol in the liver.MethodsExpression of acetylcholine receptor subunits in mouse liver was determined by RNA sequencing (RNA‐seq). α4 nAChR knockout (α4 KO) mice were generated in C57BL/6J mice by introducing a mutation encoding an early stop codon in exon 4 of Chrna4, the gene encoding the α4 subunit of the nAChR. The presence of the inactivating mutation was established by polymerase chain reaction and genomic sequencing, and the lack of α4 nAChR function was confirmed in primary fibroblasts isolated from the α4 KO mice. Wild‐type (WT) and α4 KO mice were fed the Lieber‐DeCarli diet (with 36% of calories from alcohol) or pair fed an isocaloric maltose‐dextrin control diet for a 6‐week period that included a ramping up phase of increasing dietary alcohol.ResultsChrna4 was the most abundantly expressed nAChR subunit gene in mouse livers. After 6 weeks of alcohol exposure, WT mice had elevated serum transaminases and their livers showed increased fat accumulation, decreased Sirt1 protein levels, and accumulation of markers of oxidative stress and inflammation including Cyp2E1, Nos2, Sod1, Slc7a11, TNFα, and PAI1. All these responses to alcohol were either absent or significantly attenuated in α4 KO animals.ConclusionTogether, these observations support the conclusion that activation of α4 nAChRs by alcohol or one of its metabolites is one of the initial events promoting the accumulation of excess fat and expression of inflammatory mediators. Thus, α4 nAChRs may represent viable targets for intervention in chronic alcohol‐related liver disease.
Financial statement comparability and audit pricing
Authors: Sun Jinghui , Li Liuchuang , Qi Baolei 
Published: June 2022

AbstractThis study examines the impact of financial statement comparability on audit pricing. Using the comparability measures of De Franco, Kothari and Verdi, we find that auditors charge lower audit fees for clients with greater financial statement comparability. This negative relation is more pronounced when auditors are industry specialists or clients in industries with higher operational heterogeneity. We also find that clients with greater financial statement comparability have lower audit risk, and their auditors exert less audit effort to provide assurance. Our study contributes to the current literature by documenting that homogeneity in financial statement reporting decreases audit risk and increases audit efficiency, reducing audit pricing.
Fish thieves: An alternative tactic of food capture in a Neotropical frugivorous species (Brycon falcatus)
Authors: Carvalho Lucélia Nobre , dos Santos Júnior João Batista , Correa Sandra Bibiana 
Published: June 2022

AbstractFrugivorous fishes switch their diets seasonally in response to fluctuating food availability; a strategy that maximizes energy and nutrient intake and reduces competition for food. Kleptoparasitism is a form of competition that involves the stealing of already‐procured items, for which the host has invested energy in prey capture. We did not find previous studies on kleptoparasitism among Neotropical fish. Here we contribute the first record of kleptoparasitism among a frugivorous fish species. The matrinxã (Brycon falcatus) is a member of an iconic genus of broadly distributed frugivorous fishes. We made focal daylight underwater observations (by snorkeling) of frugivorous fish behavior in an Amazonian stream. Opportunistic feeding interactions between a school of juvenile matrinxã, (B. falcatus) and an individual of threespot leporinus (Leporinus friderici) were observed. The matrinxã stole the fruit that was captured in the substrate by the leporinus. Brycon falcatus usually lives between the middle of the water column to the surface of rivers and streams while Leporinus friderici occupies the lower portion of the water column and it actively forages close to the substrate. This suggests that stealing food from a benthic feeder is an opportunistic ecological interaction to take advantage of scarce resources during the period of food scarcity. This alternative technique of capturing fruits may be advantageous (i.e., save energy expenditures related to searching) for young matrinxã who do not eat fruit as frequently as adults. Our results reflect the trophic plasticity and foraging opportunism characteristic of most tropical freshwater fish. We believe that the hydrological period in which the observations were made, when a few trees were bearing fruit, can favor fruit stealing by Brycon falcatus.
A frameshift variant in the EXT1 gene in a feline leukemia virus‐negative cat with osteochondromatosis
Authors: Fujii Yuji , Uno Akihiro , Takitani Shinichi , Iwasaki Ryota , Yoshikawa Ryutaro , Okajima Misuzu , Makino Yasushi , Ito Naoto , Mori Takashi 
Published: June 2022

AbstractOsteochondromatosis is a benign proliferative disorder characterized by cartilage‐capped bony protuberances. In humans and most mammals, variants in the EXT1 or EXT2 gene are strongly correlated with the etiology of osteochondromatosis. However, in cats, osteochondromatosis has only been associated with feline leukemia virus infection. In this study, to explore other factors involved in the etiology of feline osteochondromatosis, we examined the EXT1 and EXT2 genes in a feline leukemia virus‐negative cat with osteochondromatosis. Genetic analysis revealed a heterozygous single base pair duplication in exon 6 of the EXT1 gene (XM_023248762.2:c.1468dupC), leading to a premature stop codon in the EXT1 protein. Notably, this frameshift variant is recognized as one of the most common pathogenic variants in human osteochondromatosis. Our data suggest for the first time that genetic variants can have etiologic roles in osteochondromatosis in cats, as in humans and other animals.
Introduction of diabetic retinopathy screening into an antenatal clinic: Impact on maternal screening and diagnosis rates
Authors: Phillips Jessica L. , Raja Vignesh , Mehrotra Chhaya , Richards Josephine , Khan Jane , Graham Dorothy F. 
Published: June 2022

AbstractPregnancy is a risk factor for the development and progression of diabetic retinopathy (DR) in women with pre‐gestational diabetes. However, a minority of pregnant women with diabetes adhere to retinal screening recommendations. The introduction of an onsite retinal camera at our tertiary maternity hospital significantly increased the proportion of women who received at least one retinal screen during pregnancy (93.0% vs 54.3%, P < 0.001) and the identification of both DR and DR progression. We conclude that the use of a retinal camera in similar clinics is a feasible option to improve DR screening and diagnosis rates in pregnancy.
Peripheral chemoreflex activation induces expiratory but not inspiratory excitation of C1 pre‐sympathetic neurones of rats
Authors: da Silva Melina P. , Spiller Pedro F. , Paton Julian F. R. , Moraes Davi J. A. 
Published: June 2022

AbstractAimsStimulation of peripheral chemoreceptors, as during hypoxia, increases breathing and respiratory‐related sympathetic bursting. Activation of catecholaminergic C1 neurones induces sympathoexcitation, while its ablation reduces the chemoreflex sympathoexcitatory response. However, no study has determined the respiratory phase(s) in which the pre‐sympathetic C1 neurones are recruited by peripheral chemoreceptor and whether C1 neurone activation affects all phases of respiratory modulation of sympathetic activity. We addressed these unknowns by testing the hypothesis that peripheral chemoreceptor activation excites pre‐sympathetic C1 neurones during inspiration and expiration.MethodsUsing the in situ preparation of rat, we made intracellular recordings from baroreceptive pre‐sympathetic C1 neurones during peripheral chemoreflex stimulation. We optogenetically activated C1 neurones selectively and compared any respiratory‐phase‐related increases in sympathetic activity with that which occurs following stimulation of the peripheral chemoreflex.ResultsActivation of peripheral chemoreceptors using cytotoxic hypoxia (potassium cyanide) increased the firing frequency of C1 neurones and both the frequency and amplitude of their excitatory post‐synaptic currents during the phase of expiration only. In contrast, optogenetic stimulation of C1 neurones activates inspiratory neurones, which secondarily inhibit expiratory neurones, but produced comparable increases in sympathetic activity across all phases of respiration.ConclusionOur data reveal that the peripheral chemoreceptor‐mediated expiratory‐related sympathoexcitation is mediated through excitation of expiratory neurones antecedent to C1 pre‐sympathetic neurones; these may be found in the Kölliker‐Fuse nucleus. Despite peripheral chemoreceptor excitation of inspiratory neurones, these do not trigger C1 neurone‐mediated increases in sympathetic activity. These studies provide compelling novel insights into the functional organization of respiratory–sympathetic neural networks.
Organizational and vocational behavior in times of crisis: A review of empirical work undertaken during the COVID‐19 pandemic and introduction to the special issue
Authors: Newman Alexander , Eva Nathan , Bindl Uta K. , Stoverink Adam C. 
Published: June 2022

AbstractThe challenges faced by individuals and organizations during the COVID‐19 crisis have significantly changed how businesses operate. In response, how we think about organizational and vocational behavior research has shifted. Questions of how leaders manage their workforce, how workers manage their daily work demands, and how workers consider their career options during and post the pandemic have attracted scholars' interest to assist businesses with recalibrating to a “new normal.” Presented in this special issue are 10 original articles that seek to propel the organizational and vocational behavior fields forward, addressing questions important in the context of crises, on working life. In our introduction to the special issue, we set the scene for this new research by reviewing the organizational and vocational behavior literature published over the first 2 years of the pandemic that has explored the impact of the COVID‐19 crisis on organizations and workers. This existing body of research covers emotions, work attitudes and behaviors, health and well‐being, and team and career‐related outcomes in the context of the crisis. The special issue also reflects on the enduring challenges for organizational and vocational behavior scholars conducting research during times of crisis to provide a roadmap for future research.
Intercellular adhesion molecule‐1‐targeted near‐infrared photoimmunotherapy of triple‐negative breast cancer
Authors: Fukushima Hiroshi , Kato Takuya , Furusawa Aki , Okada Ryuhei , Wakiyama Hiroaki , Furumoto Hideyuki , Okuyama Shuhei , Kondo Eisaku , Choyke Peter L. , Kobayashi Hisataka 
Published: June 2022

AbstractTriple‐negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, and conventional chemotherapy and molecular‐targeted therapies show limited efficacy. Near‐infrared photoimmunotherapy (NIR‐PIT) is a new anticancer treatment that selectively damages the cell membrane of cancer cells based on NIR light‐induced photochemical reactions of the antibody (Ab)‐photoabsorber (IRDye700Dx) conjugate and the cell membrane. TNBC is known to express several adhesion molecules on the cell surface providing a potential new target for therapy. Here, we investigated the therapeutic efficacy of intercellular adhesion molecule‐1 (ICAM‐1)‐targeted NIR‐PIT using xenograft mouse models subcutaneously inoculated with two human ICAM‐1‐expressing TNBC cell lines, MDAMB468‐luc and MDAMB231 cells. In vitro ICAM‐1‐targeted NIR‐PIT damaged both cell types in a NIR light dose‐dependent manner. In vivo ICAM‐1‐targeted NIR‐PIT in both models showed early histological signs of cancer cell damage, such as cytoplasmic vacuolation. Even among the cancer cells that appeared to be morphologically intact within 2 h post treatment, abnormal distribution of the actin cytoskeleton and a significant decrease in Ki‐67 positivity were observed, indicating widespread cellular injury reflected in cytoplasmic degeneration. Such damage to cancer cells by NIR‐PIT significantly inhibited subsequent tumor growth and improved survival. This study suggests that ICAM‐1‐targeted NIR‐PIT could have potential clinical application in the treatment of TNBC.