bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ligands tris(2-pyridylmethyl)amine with nitrile group in α-substituted and the corresponding FeCl2 complexes
Authors: Thallaj Nasser 
Published: March 2022

Abstract
We have synthesized the mono, bis and tris nitrile at the α position ligands in the tris(2-pyridylmethyl) amine (TPA) series, respectively CNTPA, (CN)2TPA and (CN)3TPA. Nitrile at the α position of these nitrogen-containing tripods shifts the oxidation potential of the ligand by comparison with the TPA ligand, it is observed that the oxidation potentials of the ligands increase regularly with the number of nitrile groups. For each group added, the oxidation potential is increased by an average of 110mV / ECS. This reflects the strong electron-withdrawing effect of the nitrile groups.. The crystal structure analysis of the dichloroferrous complexes with CNTPA and (CN)2TPA reveals that the iron lies in a distorted octahedral geometry comparable to that already found in TPAFeCl2. All spectroscopic data indicate that the geometry is retained in solution. These three isostructural complexes all react with molecular dioxygen to yield µ-oxo diferric complexes as stable compounds. Upon reduction, all µ-oxo diferric complexes convert back into the starting ferrous species. The oxygenation reaction parallels the well known formation of µ-oxo derivatives from dioxygen in chemistry of porphyrins reported almost three decades ago. The crystal structure analysis is reported for each of these three µ-oxo compounds. With TPA, a symmetrical structure is obtained for a dicationic compound, the tripod coordinating in the (κ4-N) coordination mode. With CNTPA, the compound is a neutral µ-oxo di-ferric complex. When oxygenation is carried out on the (CN)2TPA complex, a neutral asymmetrical compound is obtained. The striking point within the series of ferrous precursors is the effect of the ligand on the kinetics of oxygenation of the complexes. Whereas the parent complex undergo full oxygenation over 14 hours, the mono nitrile ligand provides a complex that has fully reacted over 14 hours, the reaction time being only 2 hours for the complex with the dinitriles ligand,and for trinitriles ligand it will tack 30min. Thus electron-deficiency ( Lewis acidity at the metal centre) seems to govern the kinetics of oxygenation. This strongly suggests coordination of the dioxygen as an initial step in the process leading to formation of µ-oxo diferric compounds, by contrast with un unlikely outer sphere reduction of dioxygen, that generally occurs at very negative potentials. Finally, we report that cyclohexane is catalytically converted into cyclohexanone when reacted with dioxygen in the presence of zinc amalgam, and that the conversion rate also parallels the Lewis acidity at the metal center, with the best rate obtained for with (CN)2TPAFeCl2.
The Relationship Between Cumulative Ecological Risk and Adolescent Cyberbullying: the Mediating Effects of Rumination and Cybervictimization
Authors: Liu Yifan , Gan Xiong , Liu Rong , Jin Xin , Zhu Congshu 
Published: March 2022

Abstract
This study explored the mechanism of cyberbullying, the independent mediating effect of rumination and cybervictimization respectively, and the chain mediation effect of rumination and cybervictimization based on the theory of cumulating ecological risk. 1510 junior high school students (mean age:15.2, standard deviation: 1.41, ag range: 13–17) were adopted to fill with the Cyberbullying/Cybervictimization Questionnaire, the Cumulative Ecological Risk Scale, the Rumination Scale. And we tested the correlation among the four variables, namely rumination, cybervictimization, cumulative risk, and cyberbullying. The chain mediating effect of rumination and cybervictimization between cumulative ecological risk and cyberbullying was modelled as well. The results revealed that: (1) cumulative ecological risk had a significant positive predictive effect on cyberbullying behaviour with controlling for grade and gender. (2) Cybervictimization mediated the relationship between cumulative ecological risk and cyberbullying; (3) Rumination and cyber-victimization play a chain mediating role in the relationship between cumulative ecological risk and cyberbullying.
CD44+/CD24-Flow Cytometry and Immunohistochemistry on Therapy Response to the Administration of Chemotherapy in Ovarian Cancer
Authors: Sihombing Unedo Hence Markus , Andrijono Andrijono , Purwoto Gatot , Gandamihardja Supriadi , Harahap Alida R. , Rustamadji Primariadewi , Kekalih Aria , Widyawati Retno , Fuady Dzicky Rifqi 
Published: March 2022

Abstract
Ovarian cancer is one of the deadliest women’s cancers. There is much chemoresistance to ovarian cancer standard therapy. CD44+/CD24− have an essential role in developing chemoresistance and poor prognosis for the patients. We aimed to study CD44+/CD24− relationship with ovarian cancer chemoresistance and its ability to predict chemoresistance. This study is an ambispective cohort study of 32 people in each group at the Obstetrics-gynecology and pathology Department of Cipto Mangunkusumo, Tarakan, Dharmais, and Fatmawati Hospital. All suspected ovarian cancer patients will undergo cytoreductive debulking and histopathological examination. Chemotherapy will be given for six series followed by six months of observation. We determine therapy response with the RECIST Criteria (Response Criteria in Solid Tumours) then classify it into chemo-resistant or chemo-sensitive groups. Our study is the first study examining CD44+/CD24− in ovarian cancer from flow cytometry blood test and directly from ovarian cancer tissue by double immunohistochemistry. We found a significant relationship between increased levels of CD44+/CD24− expression (p < 0,05) with chemoresistance of ovarian cancer from both studies while immunohistochemistry has a better multivariate analysis result. Both studies indicate that CD44+/CD24− was significantly associated with ovarian cancer chemoresistance and CD44+/CD24− immunohistochemistry is a better predictor.
Evaluation of change in the peat soil properties affected by different fire severities
Authors: Fulazzaky Mohamad Ali , Ismail Ibrahim , Harlen Harlen , Sukendi Sukendi , Roestamy Martin , Siregar Yusni Ikhwan 
Published: March 2022

Abstract
Tropical peatland ecosystems of Indonesia provide direct economic benefits to the local communities and act to maintain the local weather patterns. The impact of burning tropical peat swamp forests of land clearing for palm oil plantations can have significant consequences on the change in the characteristics of peat soil. The aim of this study was to determine the physical, chemical, and biological properties of the peat soils by the field and laboratory testing and analysis for understanding a change in the nature and characteristics of the peatlands at four locations in the Pelalawan regency of Riau province. The results showed that the effect of burning peat swamp forests can lead to a change in the physical, chemical, and biological properties of the peat soils. Soil permeability and soil microbial population can significantly decrease with increasing of the fire severity. The effect of different fire severities on the characteristics of peat soil are verified to contribute to advanced management of the tropical peatland in the future.
Multi-tissue metabolomic profiling reveals potential biomarkers of cocoon yield in silkworms (Bombyx mori) fed formula feed versus mulberry leaves
Authors: Wu Xuehui , Chen Xuedong , Ye Aihong , He Ruimin , Zhang Meihua , Pan Meiliang , Jin Feng , Ma Huanyan , Cao Jinru , Zhou Wenlin 
Published: March 2022

Abstract
Background
Use of formula feed (FF) for silkworms for all instars, has promoted transformation and progress in traditional sericulture. However, the cocoon yield of FF silkworms has failed to reach that of silkworms fed mulberry leaves (ML). The biological mechanisms underlying this phenomenon have not been well described. This study aimed to identify potential biomarkers and metabolic mechanisms relating to the poor cocoon yield of FF silkworms. In this study, silkworms received treatments of either ML (ML group) or FF (FF group) for all instars. At the 3rd day of the 5th instar, the hemolymph, midgut and posterior silk gland were collected for the metabolome profiles detection. The remaining silkworms were fed ML or FF until cocooning for investigation.
Results
The whole cocoon yield (WCY) was significantly higher in the FF group than the ML group (P < 0.05), whereas the cocoon shell weight (CSW) and cocoon shell rate (CSR) were significantly lower in the FF group (P < 0.05). A total of 867, 845 and 831 metabolites were qualified and quantified in the hemolymph, midgut and posterior silk gland of the FF silkworms, respectively. Correspondingly, 833, 789 and 730 metabolites were quantified in above three tissues of the ML group. Further, 249, 230 and 304 significantly different metabolites (SDMs) were identified in the hemolymph, midgut and posterior silk gland between the FF and ML group, respectively. Eleven metabolic pathways enriched by the SDMs were mutual among the three tissues. Among them, cysteine and methionine metabolism, arginine biosynthesis, and arginine and proline metabolism were the top three pathways with the highest impact value in the posterior silk gland. Six biomarkers were obtained through biomarker analysis and Pearson correlation calculation. Among them, homocitrulline, glycitein, valyl-threonine, propyl gallate and 3-amino-2,3-dihydrobenzoic acid were positively correlated with WCY, but negatively correlated with CSW and CSR (P < 0.05). An opposite correlation pattern was observed between 3-dimethylallyl-4-hydroxyphenylpyruvate and the three cocoon performance traits.
Conclusions
Overall, three key metabolic pathways and six biomarkers associated with cocoon yield were interpreted, and should provide directions for formula feed optimization in factory-raised silkworms.
Faculty perceptions and use of e-learning resources for medical education and future predictions: a cross sectional study
Authors: Kim Kyong-Jee , Kim Giwoon , Kang Youngjoon 
Published: March 2022

Abstract
Background: The remarkable increase in the use of online teaching and learning in medical education caused by the COVID-19 pandemic, research on faculties’ perceptions and the use of e-learning resources has been scant. This study aims to investigate the use of e-learning resources by medical faculties and explore future directions in using and sharing of such resources.Methods: This descriptive study was conducted on full-time faculty members who were registered users of the e-learning portal of the consortium of 36 Korean medical schools. Participants were invited to an online survey containing 45 items that addressed their perceptions and use of e-learning resources, and their predictions of future use. Descriptive analysis and reliability analysis were conducted as well as a thematic analysis of qualitative data.Results: Ninety faculty members from 31 medical schools returned the questionnaires. Respondents had positive perceptions of e-learning resources and predicted that the use of such resources would become more popular. However, only a half of the respondents used e-learning resources for teaching, and they pointed out several barriers to the use and sharing of the resources. Furthermore, our findings suggest future directions in terms of content areas that we should focus on creating and sharing resources, namely, multimedia assessment items, clinical videos, virtual patients, sharing best video lectures across institutions, and Massive Open Online Courses (MOOCs).Conclusions: Medical schools need to provide faculty support to overcome barriers that inhibit faculty use and sharing of e-learning resources. Our findings suggest future directions in terms of content areas that we should focus on creating and sharing resources in order to respond to current challenges in medical education.
Structure-Guided Engineering of Immunotherapies Targeting TRBC1 and TRBC2 in T Cell Malignancies
Authors: Ferrari Mathieu , Baldan Vania , Wawrzyniecka Patrycja , Bulek Anna , Kinna Alex , Ma Biao , Bugda Reyisa , Akbar Zulaikha , Srivast Saket , Ghongane Priyankha , Gannon Isaac , Robson Matthew , Sillibourne James , Jha Ram , Lim Wen Chean , Hopkins Jade , Welin Martin , Surade Sachin , Dyson Michael , McCafferty John , Cordoba Shaun , Thomas Simon , Logan Derek , Sewell Andy , Maciocia Paul , Onuoha Shimobi , Pule Martin 
Published: March 2022

Abstract
Peripheral T cell lymphomas are typically aggressive with a poor prognosis. Unlike other hematologic malignancies, the lack of target antigens to discriminate healthy from malignant cells has limited the efficacy of immunotherapeutic approaches. The T cell receptor expresses one of two highly homologous chains [T cell receptor β-chain constant (TRBC) domains 1 and 2] in a mutually exclusive manner, making it a promising target. We previously described an antibody with unique TRBC1 specificity (Jovi-1). Here we demonstrate specificity redirection by rational design using structure-guided computational biology to generate a TRBC2-specific antibody (KFN). This permitted the generation of paired reagents (chimeric antigen receptor-T cell) specific for TRBC1 and TRBC2, with preclinical evidence to support their efficacy in T cell malignancies.
Development of a short-form Chinese Health Literacy Scale For Low Salt Consumption (CHLSalt-22) and its validation among hypertensive patients
Authors: Zhang Yanli , Zhang Hanjing , Li Song , Li Yuetong , Hu Cunjie , Li Hongyu 
Published: March 2022

Abstract
Background:With the accelerated pace of people's life and the changed dietary patterns, the number of chronic diseases is increasing and occurring at a younger age in today's society. The speedily rising patients with hypertension led it to becoming one of the main risk factors for chronic diseases. People should focus on health literacy related to salt consumption and reach a better quality of life. At present, there is a lack of local assessment tools for low salt consumption in mainland China. Objective:To develop a short-form version of Chinese Health Literacy Scale For Low Salt Consumption instrument for using in mainland China. Methods:A cross-sectional design was conducted in a sample of 1472 people in Liaoxi, China. Participants completed a sociodemographic questionnaire, the Chinese version of the CHLSalt-22, the measuring change in restriction of salt (sodium) in diet in hypertensives (MCRSDH-SUST), the Brief Illness Perception Questionnaire (BIPQ) and the Benefit-Finding Scales (BFS) to test the hypothesis. Exploratory factor analysis and confirmatory factor analyses were performed to examine the underlying factor structure of the CHLSalt-22. One month later, 37 patients who participated in the first test were recruited to evaluate the test–retest reliability. Results:The CHLSalt-22 demonstrated adequate internal consistency, good test–retest reliability and satisfactory construct validity, convergent validity and discriminant validity. The CHLSalt-22 count scores were correlated with age, sex, body mass index (BMI), education level, income, occupation, the Measuring Change in Restriction of Salt (sodium) in Diet in Hypertensives (MCRSDH-SUST), the Brief Illness Perception Questionnaire (BIPQ), and the Benefit-Finding Scales (BFS). Conclusion:The results indicate that the version of the Chinese Health Literacy Scale For Low Salt Consumption (CHLSalt-22) has good reliability and validity, and that it can be considered a tool to assess health literacy related to salt consumption in health screenings.
Engineering Branching Morphogenesis Using Cell Communication
Authors: Marquette Christophe , Devillard Chloe 
Published: March 2022

Abstract
Branching morphogenesis, a specialized part of morphogenesis, leads to the formation of microstructures (tubes, canals, and glands), source of the active organ functions. The dynamic mechanisms involved are appearance/disappearance of biomolecules morphogens gradients. In the context of angiogenesis, growth factors allow the initiation, regulation, and remodeling of blood vessels. In the particular case of micro-vascularization, it seems essential to reproduce and study the interaction of endothelial cells with their environment but also with other cellular components, including fibroblasts. To bring understanding here, we developed an angiogenesis 3D bioprinted model based on a proliferative bioink populated with fibroblasts and HUVECs. We demonstrated that we were able to recapitulate branching angiogenesis, producing organized microvascularization in 7 days only. We clearly demonstrated that a bidirectional communication was at stake between the two cell types, evidenced only when both types were culture in a 3D environment. Proteomic results consolidated the understanding of this phenomenon, with 11 angiogenic proteins identified in the co-culture supernatant. They were identified as inducers of vasculogenesis and angiogenesis. Through matrix composition and cell organization study, we were able to demonstrate that tissue remodeling, extracellular matrix production (type I collagen), phenotype modification (pericytes) were taking place in our branching morphogenesis model.
Utilization of A Meteorological Satellite for Planetary Science as A Space Telescope: The Lunar Mid-Infrared Spectrum as Seen by Himawari-8
Authors: Nishiyama Gaku , Namiki Noriyuki , Sugita Seiji , Uno Shinsuke 
Published: March 2022

Abstract
The Japanese meteorological satellite Himawari-8 has been capturing not only the earth’s atmosphere, but also stars, planets, and the moon in its field of view and enables us to take their spectroscopy with 16 bands from visible to mid-infrared wavelengths. Especially for the lunar case, nine infrared bands equipped in the Advanced Himawari Imager (AHI) onboard Himawari-8 are unique in spaceborne observations and potentially useful for lunar science. Besides, the infrared bands of AHI cover similar wavelengths with those of other interplanetary instruments, thus are useful for their calibrations. However, the infrared AHI data have not been investigated in planetary science. To confirm the utility of AHI for planetary science, we develop the procedure to retrieve the lunar infrared spectrum and compare it with thermal conduction simulations. Our analysis shows that the lunar brightness temperature curves can be obtained in the morning, evening, and night sides at all nine bands. Particularly at 8.5 μm, they show a good agreement with the previous observation by the Diviner radiometer onboard NASA's Lunar Reconnaissance Orbiter. As pointed out previously, the brightness temperatures differ between bands, indicating the temperature mixing within a pixel. In the morning and evening, our simulation suggests that surface roughness as steep as those measured at Apollo landing sites significantly contributes to the observed brightness temperature differences. On the other hand, the nightside brightness temperatures are greatly affected by rocks that have higher thermal inertia than regolith. The rock abundances are estimated as 0.18 –0.48 and 5.6 –10.4 % at the equator and within Tycho crater, respectively. Our estimations from the AHI data are consistent with one from Diviner. These results support that AHI potentially serves as a space telescope for future lunar and planetary sciences, for example, to constrain water content on the lunar surface.
The circSPON2/miR-331-3p axis regulates PRMT5, an epigenetic regulator of CAMK2N1 transcription and prostate cancer progression
Authors: Feng Ninghan , Yao Bing , Zhu Sha , Wei Xiyi , Chen Ming-Kun , Feng Yangkun , Li Zhimin , Xu Xinyu , Zhang Yuwei , Wang Yang , Zhou Jingwan , Tang Ningyuan , Ji Chengjian , Jiang Peng , Zhao Shan-Chao , Qin Chao 
Published: March 2022

Abstract
Background: Prostate cancer (PCa) is the most frequently diagnosed malignancy in men, and its mechanism remains poorly understood. Therefore, it is urgent to discover potential novel diagnostic biomarkers and therapeutic targets that can potentially facilitate the development of efficient anticancer strategies.Methods: A series of functional in vitro and in vivo experiments were conducted to evaluate the biological behaviors of PCa cells. RNA pulldown, Western blot, luciferase reporter, immunohistochemistry and chromatin immunoprecipitation assays were applied to dissect the detailed underlying mechanisms. High-throughput sequencing was performed to screen for differentially expressed circRNAs in PCa and adjacent normal tissues.Results: Upregulation of protein arginine methyltransferase 5 (PRMT5) is associated with poor progression-free survival and the activation of multiple signaling pathways in PCa. PRMT5 inhibits the transcription of CAMK2N1 by depositing the repressive histone marks H4R3me2s and H3R8me2s on the proximal promoter region of CAMK2N1, and results in malignant progression of PCa both in vitro and in vivo. Moreover, the expression of circSPON2, a candidate circRNA in PCa tissues identified by RNA-seq, was found to be associated with poor clinical outcomes in PCa patients. Further results showed that circSPON2 induced PCa cell proliferation and migration, and that the circSPON2-induced effects were counteracted by miR-331-3p. Particularly, circSPON2 acted as a competitive endogenous RNA (ceRNA) of miR-331-3p to attenuate the repressive effects of miR-331-3p on its downstream target PRMT5. Conclusions: Our findings showed that the epigenetic regulator PRMT5 aggravates PCa progression by inhibiting the transcription of CAMK2N1 and is modulated by the circSPON2/miR-331-3p axis, which may serve as a potential therapeutic target for patients with aggressive PCa.
NUDT22 promotes cancer growth through pyrimidine salvage and the TCA cycle
Authors: Walter Melanie , Mayr Florian , Hanna Bishoy Magdy Fekry , Cookson Victoria , Mortusewicz Oliver , Helleday Thomas , Herr Patrick 
Published: March 2022

Abstract
The NUDIX hydrolase NUDT22 converts UDP-glucose into glucose-1-phosphate and the pyrimidine nucleotide uridine monophosphate1. Glucose-1-phosphate is an important metabolite for energy and biomass production through glycolysis and the subsequent tricarboxylic acid (TCA) cycle and nucleotides required for DNA replication are produced through energetically expensive de novo or energy-efficient salvage pathways2. Here, we describe p53-regulated pyrimidine and glucose-1-phosphate salvage through NUDT22-dependent hydrolysis of UDP-glucose to maintain cancer cell growth and to prevent replication stress. We identified elevated NUDT22 expression in cancer tissues and reduced TCA intermediates and oncometabolites, growth retardation, S-phase delay, and slower DNA replication forks in NUDT22-deficient cancer cells. NUDT22 transcription is induced under starvation, oncogenic stress, and DNA damage directly through p53. Uridine supplementation rescues replication fork progression and alleviates replication stress. Conversely, NUDT22 deficiency sensitizes cells to de novo pyrimidine synthesis inhibition in vitro and reduces cancer growth in vivo. In conclusion, NUDT22 maintains pyrimidine supply and provides a substrate for the TCA cycle in cancer cells. Depletion of NUDT22 leads to genome instability and a reduction in TCA cycle metabolites, including oncometabolites such as 2-hydroxyglutarate and succinate. Targeting NUDT22 therefore has high potential for therapeutic applications in cancer therapy, simultaneously interfering with two essential survival pathways.