bluuebunny/crossref_metadata_2025_split huggingface dataset:
COVID-19-induced headache in Boston and the vicinity
Authors: Chwalisz Bart K. , Westover M. Brandon , Cheng Hsinlin T. , Le Vi K. , Jain Aayushee , Cheng Jennifer R. 
Published: May 2022

Abstract
BackgroundCoronavirus disease 2019 (COVID-19) causes mild symptoms to severe illness in the nervous system. Among the wide-ranged symptomology, headache is a common neurological symptom reported by COVID-19 patients. However, the prevalence, comorbidities, and ethnic susceptibilities of COVID-19-induced headaches are not well-defined. MethodsWe performed a retrospective chart review of patients who tested positive for SARS-CoV2 by reverse transcriptase -polymerase chain reaction (RT-PCR) in March and April 2020 at Massachusetts General Hospital, Boston, Massachusetts, USA. An electronic medical record review was performed to collect demographic data, major COVID-19 symptomology, and results of radiologic and laboratory tests. Data were recorded starting on the disease onset, as identified by the patient’s clinical notes. The demographical trends and prevalence of headache and comorbid symptoms were statistically analyzed. Results We identified 450 patients, 202 (44.9%) male and 248 (55.1%) female, who tested positive for COVID-19 in the study. Common symptoms included cough (66.4%), fever,(58.9%), fatigue (30.9%), and sore throat (29.6%). Forty-six percent of patients had shortness of breath, 32% had evidence of pneumonia, and 26% needed hospitalization. Gender predominance is significant in developing a sore throat, nasal congestion, hypogeusia, headache, ear pain in females, and pneumonia and inpatient hospitalization in males. Younger patients (< 50) were more likely to develop sore throat, fatigue, anosmia, hypogeusia, ear pain, myalgia /arthralgia, and headache. In contrast, older (> 50) patients were prone to develop pneumonia and required hospitalization. Compared to non-Hispanic ethnic populations, a significantly higher number of Hispanic patients reported headaches, nausea/vomiting, nasal congestion, fever, fatigue, anosmia, and myalgia/arthralgia compared to non-Hispanics, suggesting an ethnic susceptibility in COVID-19 symptomatology. Overall, patients who developed headaches were younger and more likely to have mild viral symptoms, pain, and neurological symptoms but not severe respiratory symptoms (shortness of breath and pneumonia) and hospitalization. ConclusionsOur results demonstrated gender, age, and ethnic factors in COVID symptomatology and identified that mild viral, painful, and neurological symptoms are positive predictors for headache development in COVID-19.
Establishment and Validation of Prognostic Scoring Model Based on Cuproptosis-Related Long Non-Coding RNAs in Stomach adenocarcinoma
Authors: Fan Chuanlei , He Yang , Yang Jian , Lu Taofeng , Da Mingxu 
Published: May 2022

Abstract
Background: Long non-coding RNAs ( lncRNAs) are pivotal regulatory factors in gastric cancer advance and affect patients' prognosis. Cuproptosis-related lncRNAs (CRLs) have been implicated in the advancement of lung and liver cancers, but not gastric cancer. Therefore, identifying CRLs with predictive relevance in gastric cancer is critical.Methods: The Cancer Genome Atlas (TCGA) provided RNA sequencing and clinical data, whereas genes were collected from other published publications. We assigned TCGA data to two groups: training and testing. For training cohort, least absolute shrinkage and selection operator (LASSO), univariate and multivariate Cox regression analysis were used to create a prognostic CRLs model, while testing cohort and entire cohort were administered to authenticate prognostic signature. Likewise, nomogram was utilized to forecast the outcome of various clinical pathologic phases and risk scores. Finally, a Co-expression network was used to find lncRNAs linked to cuproptosis.Results: On the basis of univariate Cox regression analysis, the prognostic value of 48 lncRNAs was screened and these lincRNAs were also evaluated using LASSO, as well as multivariate Cox regression analysis. LINC00968 and LINC01579, two lncRNA prognostic risk models, were created. The survival time of STAD patients in high-risk groups,according to the results, had a patently worse outcome than low-risk ones in training cohort, which was consistent with the results of test cohort and entire cohort. Combined with clinicopathological characteristics, univariate and multivariate Cox regression analysis suggested this risk model represented a novel independent predictive factor for gastric cancer. Furthermore, Normogram confirmed that the prediction results of this model are accurate and reliable. Conclusion: LINC00968 and LINC01579 have a substantial predictive value in gastric cancer and could be a latent therapeutic target in the clinic.
Assessment of Selected heavy Metals in Honey Samples Using Atomic Absorption Spectroscopy (AAS), Ethiopia
Authors: Tibebe Dessie , Hussen Mohammed , Mulugeta Marye , yenealem Dereje , Moges Zerubabile , Gedefaw Mohammed , Kassa Yezbie 
Published: May 2022

Abstract
Honey is a natural, sugary and sticky liquid that is produced from the nectars of flowers. This study aimed to analyze the concentration of some selected heavy Metals (Zn, Cu, Mn, Cd, Pb, and Cr) in honey samples. 1g of honey sample was digested by a hot plate using 9ml of HNO3 and 3ml of H2O2. The concentrations of the heavy metals in the digested were detected using a flame atomic absorption spectrometer. The results of this study found that the concentrations of the heavy metals in the honey samples were ranged from 1.97 to 2.04 µg/g for Zn, 1.93µg/g to 2µg/g for Cu, 0.83 to 1.01 µg/g for Mn, 0.25 to 0.45 µg/g for Cr, (0.025–0.031 µg/g for Cd. However, Pb was not detected in all honey samples. Hence, the levels of heavy metals found were below the permitted levels set by the World Health Organization.The contamination levels of the heavy metals are also found to be below the detection levels. The results of this study helped us to know that the acceptable amounts of heavy metals present in the honey samples. From the results, the levels of heavy metals found were below the permitted levels set by the World Health Organization. Thus, the heavy metals in the sampled honey are safe for human consumption in these selected areas.
Experimental considerations of acute heat stress assays to quantify coral thermal tolerance
Authors: Nielsen JJV , Matthews G , Frith KR , Harrison HB , Marzonie  , Slaughter KL , Suggett DJ , Bay LK 
Published: May 2022

Abstract
Thermal tolerance is variable in corals, yet intrinsic and extrinsic drivers of tolerance are not well understood. Understanding the distribution and abundance of heat tolerant corals across seascapes is imperative for predicting responses to climate change and to support novel management actions. Rapid and high-throughput methods to measure heat-induced coral bleaching sensitivity are increasingly required to understand current and predict future responses to climate change. Experimental evaluations of coral heat and bleaching tolerance typically involve ramp-and-hold experiments run across days to weeks within aquarium facilities with limits to colony replication. Field-based acute heat stress assays have emerged as an alternative experimental approach to rapidly quantify heat tolerance in a large number of samples yet the role of key methodological considerations on the stress response measured remains unresolved. Here, we quantify the effects of coral fragment size, sampling time point, and physiological measures on the acute heat stress response in adult corals. The effect of fragment size differed between species (Acropora tenuis and Pocillopora damicornis). Most physiological parameters measured here declined over time (tissue colour, chlorophyll-a and protein content) from the onset of heating, with the exception of maximum photosynthetic efficiency (Fv/Fm), which was stable up to 24h post heating. Based on our experiments, we identified photosynthetic efficiency, tissue colour change, and host-specific assays such as catalase activity as key physiological measures for rapid quantification of thermal tolerance. We recommend that future applications of acute heat stress assays include larger fragments (>9cm2) where possible and sample between 10 - 14h after the end of heat stress. A validated high-throughput experimental approach combined with cost-effective genomic and physiological measurements underpins the development of markers and maps of heat tolerance across seascapes and ocean warming scenarios.
Live Attenuated Measles, Mumps and Rubella Booster Vaccine in Children Diagnosed with Rheumatic Disease: A single-center study
Authors: Çakmak Figen , Akgün Özlem , Demirkan Fatma Gül , Tanatar Ayşe , Kayaalp Gülşah Kavrul , Keskindemirci Gonca , Guliyeva Vafa , Ömeroğlu Rukiye Eker , Gökçay Emine Gülbin , Ayaz Nuray Aktay 
Published: May 2022

Abstract
PurposeTo evaluate the safety profile of measles, mumps and rubella (MMR) booster in children diagnosed with rheumatic diseases receiving biological agents.MethodsThe study included retrospective safety data of children administered MMR booster dose receiving biologics or biologics with methotrexate. The files of 182 patients were accessed from the pediatric rheumatology biological therapy archive and the vaccination status of these children was obtained by accessing electronic records.ResultsOf 182 patients 14 patients were vaccinated with MMR booster dose. Thirteen of the patients were followed up with a diagnosis of juvenile idiopathic arthritis and one with colchicine-resistant familial Mediterranean fever. None of the patients had disease exacerbation after vaccination and three patients had mild side effects consisting of rash, angioedema, joint pain, and fatigue.ConclusionThis study supports the data regarding evidence of the safety of MMR booster dose administration in children with rheumatic diseases receiving bDMARDs.
Short-term morpho-functional changes in previously treated neovascular AMD eyes switched to brolucizumab
Authors: Viggiano Pasquale , Grassi Maria Oliva , Boscia Giacomo , Pignataro Mariagrazia , Petruzzella Giovanni , Borrelli Enrico , Molfetta Teresa , Alessio Giovanni , Boscia Francesco 
Published: May 2022

Abstract
Purpose: To explore the morpho-functional fluctuations in eyes treated for nAMD when switched from aflibercept or ranibizumab to brolucizumab.Methods: 31 eyes of 31 patients with nAMD with type 1 MNV were included. All patients were imaged using the SD-OCT. The OCT acquisition was performed at the following visits:(I)1 month before the intravitreal injection of aflibercept or ranibizumab (T1),(I) 1 month after T1 and at the time of switch to brolucizumab injection (T2), and 1 month after the latter procedure (T3). The main outcome measures were:(1) Central macular thickness (CMT),(2) choroidal vascularity index (CVI) and,(3) subfoveal choroidal thickness (CT). Results: CMT analysis showed fluctuations at 3 times. In detail,T2 displayed a thicker CMT in comparison to T1, although not statistically significant (p= 0.12). Contrariwise,T3 showed a thinner CMT in comparison to T2 (p= 0.002). Analyzing CVI among the three different times, the LCA and TCA showed significantly different values before and after switching to brolucizumab. T2 showed a significant reduction in both vessel lumen and total area compared to T1 (p= 0.032 and p = 0.046, respectively).Moreover, T3 showed a greater value of both LCA and TCA in comparison to T2 (p= 0.008 and p = 0.01, respectively).CT did not show significant differences at each time (P>0.05)Conclusions: Our results reported early experiences on morpho-functional fluctuations in patients with nAMD switched to brolucizumab. The anatomical impact of brolucizumab administration appears to result in more effective resolution of SRF and IRF, in association with choroidal vascular swelling.
Design of Implant Prosthesis for Bone Injury Repair Considering Stress Shielding Effect
Authors: Shao Yixin , Zhu Ye , Guan Tianmin , Hu Qi , Wei Baozhong , Lin Bing , Liyan Zhang 
Published: May 2022

Abstract
The failure of bone injury repair surgery is mostly due to the stress shielding effect caused by the difference of elastic modulus between the implant prosthesis and human bone, resulting in a great damage to patients. To solve this problem, in this study, the influencing factors of the elastic modulus of implant prosthesis were investigated, the relationship between the elastic modulus of the implanted prosthesis and the influencing factors was analyzed, and then a design method of the implant prosthesis to reduce the stress shielding effect by adjusting the unit module to control the elastic modulus was established. This method was used for the biomechanical simulation to simulate the displacement and stress distribution between the implant prosthesis and the surrounding bone tissue, and then the reliability of the method was verified. The implant prosthesis with an elastic modulus consistent with that of the experimental dog bone was made by this method, and used for the animal experiments. The effects of implant prosthesis with different modulus on the growth of surrounding bone tissue were observed, and at the same time, the reliability of the implant design method and the results of biomechanical simulation were verified. It is confirmed that this method can effectively reduce the stress concentration of implant prosthesis by more than 15.4% and increase the growth of bone tissue by more than 21%.
Fast imaging of 15 intracellular compartments and interactions by deep learning segmentation of super-resolution data
Authors: Zhanghao Karl , Li Meiqi , Chen Xingye , Liu Wenhui , Li Tianling , Wang Yiming , Su Fei , Wu Zihan , Shan Chunyan , Wu Jiamin , Zhang Yan , Fu Jingyan , Xi Peng , Jin Dayong 
Published: May 2022

Abstract
The number of colors in fluorescence microscopy is far less than the types of intracellular compartments, bringing gaps in studying live-cell anatomy and multiple organelles’ interactions. Here, we report that super-resolution imaging in association with deep convolutional neuronal networks can predict 15 subcellular structures at > 91.7% pixel accuracy using one laser excitation and two detection channels. It not only bypasses the limitations of multi-color imaging with single dye labeling but also accelerates the imaging speed by more than one order of magnitude. We find that the super-resolution ratiometric images well reflect the heterogeneity of organelles as the intrinsic “optical fingerprint” and the neuronal networks can be generalized with transfer learning to predict both 3D and 2D datasets from different microscopes, different cell types, and even complexed system of living tissues. It enables us to resolve the 3D anatomic structure of live cells at different mitotic phases and to track down the fast dynamic interactions among 9 intracellular compartments.
CENPF Knockdown Inhibits Adriamycin Chemoresistance in Triple Negative Breast Cancer mediated by Rb-E2F1-Chk1 Axis
Authors: Mu Kun , Wang Depeng , Xu Wei , Huang Minghua , Ma Wei , Liu Yulu , Zhou Xingchen , Yang Qingrui 
Published: May 2022

Abstract
Frequent developed resistance leads to early relapse and short survival in Triple-negative breast cancer (TNBC). Targeting DNA damage response (DDR) has become an effective strategy for overcoming TNBC chemoresistance. CENPF (centromere protein) is a key regulator of cell cycle progression, but its role in TNBC chemotherapy resistance remains unclear. Here we found CENPF, highly expressed in TNBC, is associated with poor prognosis in patients receiving chemotherapy. And in vitro CENPF knockdown significantly increased adriamycin (ADR)-induced cytotoxicity in MDA-MB-231 and its ADR-resistant strains (MDA-MB-231/ADR). Then we demonstrated that Rb-E2F1 axis mediates regulation of CENPF on Chk1 (Check Point kinase1) expression and CENPF promotes adriamycin chemoresistance by inducing Chk1-mediated G2/M phase arrest in TNBC. Collectively, our results indicated a novel mechanism of CENPF to regulate TNBC chemoresistance through Rb-E2F1-Chk1 axis and also suggested CENPF as a potential target for intervention to overcome chemotherapy resistance in TNBC.
Study on anti-inflammatory and healing mechanism of Jiawei Shengji Yuhong ointment on wound based on NF-κBp65 / IL-1β / VEGF pathway
Authors: Mei Pingping , Feng Wenzhe 
Published: May 2022

Abstract
Objective: To observe the effect of Jiawei Shengjiyuhong Ointment on wound repair of rat model after anal fistula surgery by regulating NF-κBp65 / IL-1β / VEGF signaling pathway. Methods : Sixty rats were divided into vaseline model group (n = 10), + Kangfuxin fluid control group (n = 10), modified Shengji Yuhong Ointment group (n = 10), and PDTC intervention model group (n = 30) (PDTC ++ Kangfuxinfluid group, PDTC + vaseline group, PDTC + modified Shengji Yuhong Ointment group, Ten rats in each group). The wound model of rats after anal fistula operation was established and intervened in different ways. The general situation and wound healing of rats in each group were recorded, and the wound healing rate of rats in each group was calculated. On the 3 th, 7 th and 14 th day, two rats in each group were randomly selected and sacrificed. The wound tissues of the same site were taken for Western blotting and immunohistochemistry to detect the protein and expression of NF-κBp65 in the wound tissues. The expression of IL-1β / VEGF was detected by elisa method. At the same time, HE staining was used to observe the histomorphology.Results : Compared with the model control group, the wound healing rate of the modified Shengji Yuhong ointment group and PDTC + modified Shengji Yuhong ointment group was the highest. HE staining results also confirmed that the wound healing degree of the modified Shengji Yuhong Ointment group and PDTC + modified Shengji Yuhong Ointment group was the best. The results of Western blotting and immunohistochemistry showed that the protein expression of NF-κBp65 in the wound tissue of rats was significantly inhibited in the Jiawei Shengji Yuhong Ointment group, PDTC + Jiawei Shengji Yuhong Ointment group and PDTC++ Kangfuxin fluid group and PDTC + Jiawei Shengji Yuhong Ointment had the best effect, and the difference was statistically significant compared with the control group and the model group (P < 0.05). The elisa method showed that the content of IL-1β in Jiawei Shengji Yuhong Ointment was lower than that in the control group and the model group (P < 0.05) at 3 day and 7 day after administration, and it showed a gradually decreasing trend. The content of IL-1β in PDTC + Jiawei Shengji Yuhong Ointment was the lowest. The content of VEGF in Jiawei Shengji Yuhong Ointment was higher than that in the control group and the model group (P < 0.05), and it showed a gradually increasing trend. The content of VEGF in PDTC + Jiawei Shengji Yuhong Ointment was the highest.Conclusion : Jiawei Shengji Yuhong Ointment can inhibit the expression of NF-κB p65 and IL-1β protein in NF-κB signaling pathway, up-regulate the expression of VEGF protein, promote epithelial cell proliferation, reduce inflammatory response, restore normal dermal fiber tissue, accelerate the recovery of wound tissue, and shorten the healing time.
Coreless optical fiber for hemoglobin (HB) sensing with bi-layer based on surface Plasmon resonance
Authors: Hamid Mustafa 
Published: May 2022

Abstract
In this work, an optical fiber biomedical sensor for detecting the ratio of the hemoglobin in the Blood is presented. A surface plasmon resonance (SPR)-based optical fiber was not developed and implemented using single and multi-mode optical fibers. The sensor is also utilized to evaluate refractive indices and concentrations of blood samples, with 40 nm thickness of (20 nm Au and 20 nm Ag) metal film of the exposed sensing region.
Assessment of Cormic Index and other Anthropometric Parameters of young adults with Sickle Cell Anaemia in Ile-Ife, Nigeria
Authors: Asafa Muritala Abiola , Bolarinwa Rahman A. , Oyewade Samson Adeoye , Ogunlade Oluwadare 
Published: May 2022

Abstract
Background: This study assessed the effect of Haemoglobin SS (Hb SS) on cormic index and some other body anthropometric indices among students of Obafemi Awolowo University, Ile-Ife, Nigeria. Methods: A total of 100 young adults (18 – 40 years) participated in the study. They were classified into two groups; 50 cases (participants with Hb SS) and 50 control (participants with Hb AA) which were purposively selected after heamglobin typing using electrophoresis method. The cases and controls were age-and sex-matched. The weight, sitting and standing height were measured by using Seca stadiometer-model 216 following standard protocol. Cormic index (CI%) was calculated using sitting height x 100/ standing height while the body mass index (BMI) and body surface area were calculated using Keys and Monsteller formulae respectively. The data were analyzed using descriptive and inferential statistics and alpha value was set at p < 0.05. Results: The mean values of weight in kg [54.39 ± 5.54 vs 60.48 ± 8.65 (t=- 4.195, p < 0.001], body mass index in kg/m2 [19.58 ± 2.08 vs 21.48 ± 2.91 (t = 6.068; p < 0.001)], cormic index in % [44.71 ± 6.05 vs 50.03 ± 1.33 (t = -3.760; p < 0.001)], subischial leg length in cm [92.22 ± 10.76 vs 83.84 ± 4.41 (p < 0.001; t = 5.092)] and body surface area in m2 [1.59 ± 0.09 vs 1.68 ± 0.14 (p < 0.001, t = -3.798)] of cases were significantly different from those of controls respectively. Conclusion: This study showed that Hb SS is associated with lower weight, sitting height, BMI, BSA and cormic index but higher subischial leg length when compared with Hb AA.
Prolactin Receptor signaling induces acquisition of chemoresistance and reduces clonogenicity in Acute Myeloid Leukemia
Authors: Cuesta-Casanovas Laia , Delgado-Martínez Jennifer , Cornet-Masana Josep M. , Carbó José M. , Banús-Mulet Antònia , Guijarro Francesca , Esteve Jordi , Risueño Ruth M. 
Published: May 2022

Abstract
Background: Development of precision medicine requires the identification of easily detectable and druggable biomarkers. Despite recent targeted drug approvals, prognosis of acute myeloid leukemia (AML) patients needs to be greatly improved, as relapse and refractory disease are still difficult to manage. Thus, new therapeutic approaches are needed. Based on in silico-generated preliminary data and the literature, the role of the prolactin (PRL)-mediated signaling was interrogated in AML. Methods: Protein expression and cell viability were determined by flow cytometry. Repopulation capacity was studied in murine xenotrasplantation assays. Gene expression was measured by qPCR and luciferase-reporters. SA-b-Gal staining was used as a senescence marker.Results: The prolactin receptor (PRLR) was upregulated in AML cells, as compared to their healthy counterpart. The genetic and molecular inhibition of this receptor reduced the colony-forming potential. Disruption of the PRLR signaling, either using a mutant PRL or a dominant-negative isoform of PRLR, reduced the leukemia burden in vivo, in xenotransplantation assays. The expression levels of PRLR directly correlated with resistance to cytarabine. Indeed, acquired cytarabine resistance was accompanied with the induction of PRLR surface expression. The signaling associated to PRLR in AML was mainly mediated by Stat5, in contrast to the residual function of Stat3. In concordance, Stat5 mRNA was significantly overexpressed at mRNA levels in relapse AML samples. A senescence-like phenotype, measured by SA-b-gal staining, was induced upon enforced expression of PRLR in AML cells, partially dependent on ATR. Similar to the previously described chemoresistance-induced senescence in AML, no cell cycle arrest was observed. Additionally, the therapeutic potential of PRLR in AML was genetically validated. Conclusions: These results support the role of PRLR as a therapeutic target for AML and the further development of drug discovery programs searching for specific PRLR inhibitors.