bluuebunny/crossref_metadata_2025_split huggingface dataset:
Naringenin potentiates anti-tumor immunity against oral cancer by inducing lymph node CD169-positive macrophage activation and cytotoxic T cell infiltration
Authors: Kawaguchi Sho , Kawahara Kenta , Fujiwara Yukio , Ohnishi Koji , Pan Cheng , Yano Hiromu , Hirosue Akiyuki , Nagata Masashi , Hirayama Masatoshi , Sakata Junki , Nakashima Hikaru , Arita Hidetaka , Yamana Keisuke , Gohara Shunsuke , Nagao Yuka , Maeshiro Manabu , Iwamoto Asuka , Hirayama Mayumi , Yoshida Ryoji , Komohara Yoshihiro , Nakayama Hideki 
Published: January 2022

AbstractThe CD169+ macrophages in lymph nodes are implicated in cytotoxic T lymphocyte (CTL) activation and are associated with improved prognosis in several malignancies. Here, we investigated the significance of CD169+ macrophages in oral squamous cell carcinoma (OSCC). Further, we tested the anti-tumor effects of naringenin, which has been previously shown to activate CD169+ macrophages, in a murine OSCC model. Immunohistochemical analysis for CD169 and CD8 was performed on lymph node and primary tumor specimens from 89 patients with OSCC. We also evaluated the effects of naringenin on two murine OSCC models. Increased CD169+ macrophage counts in the regional lymph nodes correlated with favorable prognosis and CD8+ cell counts within tumor sites. Additionally, naringenin suppressed tumor growth in two murine OSCC models. The mRNA levels of CD169, interleukin (IL)-12, and C-X-C motif chemokine ligand 10 (CXCL10) in lymph nodes and CTL infiltration in tumors significantly increased following naringenin administration in tumor-bearing mice. These results suggest that CD169+ macrophages in lymph nodes are involved in T cell-mediated anti-tumor immunity and could be a prognostic marker for patients with OSCC. Moreover, naringenin is a new potential agent for CD169+ macrophage activation in OSCC treatment.
Identification of CD105 (endoglin) as novel risk marker in CLL
Authors: Greiner Sarah M. , Märklin Melanie , Holzmayer Samuel , Kaban Kübra , Meyer Sophie , Hinterleitner Clemens , Tandler Claudia , Hagelstein Ilona , Jung Gundram , Salih Helmut R. , Heitmann Jonas S. , Kauer Joseph 
Published: January 2022

AbstractSeveral genetic and clinical markers are established as prognostic factors in chronic lymphocytic leukemia (CLL). However, additional markers are needed for risk stratification. Flow cytometric analysis is a mainstay of CLL diagnostics, thus identification of novel prognostic surface markers can improve risk assessment without increasing burden for patients and physicians. Furthermore, surface molecules preferentially expressed in high-risk cases could serve as therapeutic targets for immunotherapy. CD105 (endoglin) is a TGF-beta coreceptor and activates endothelial cells in healthy tissues and cancer. In addition, it is expressed on healthy hematopoietic precursors as well as lymphoid and myeloid leukemias. In acute myeloid leukemia (AML), a CD105 antibody is successfully applied in clinical studies. In CLL, mRNA expression of the CD105 gene ENG reportedly correlates with other risk factors but failed to show significant correlation with overall survival. However, CD105 protein expression in CLL has never been studied. We here analyzed CD105 surface expression on CLL cells from 71 patients by flow cytometry and report for the first time that substantial levels of CD105 are detectable on CLL cells in 70.4% of patients. Using receiver operating characteristics, we established a cutoff of 5.99% positive cells to distinguish between low and high CD105 levels, the latter correlating with decreased time to first treatment and overall survival. High CD105 expression further correlates with CD38 expression. Our study identified membrane expression of CD105 as a potential risk marker and therapeutic target in high-risk CLL. However, multivariant analyses of large cohorts should be performed in confirmatory studies.
A discrete element framework for the numerical analysis of particle bed-based additive manufacturing processes
Authors: Dorussen Bram J. A. , Geers Marc G. D. , Remmers Joris J. C. 
Published: January 2022

AbstractThis paper investigates the potential of the discrete element method to simulate the physics of particle bed-based additive manufacturing. This method naturally captures the discrete aspects of additive manufacturing processes, such as material addition. The proposed discrete element framework uses constitutive relations for loose powder, bonding kinematics and the thermo-mechanical behaviour of bonded particles. The mechanical bond interactions consist of beams that interconnect the particles. These beams are able to transfer forces as well as moments. The thermal conductive bond interactions assume an effective conductive area and density to account for the voids in the system. Simulated compression tests reveal that the macroscopic Young’s modulus and Poisson’s ratio of the bonded material are controlled by only two micro-scale parameters. Furthermore, a heat conducting rod of both powder and bonded material is simulated and compared to a continuum finite element simulation. The proposed discrete model is able to simulate a complete printing process, capturing the solid material behaviour accurately. A simulation of a printed sample shows various additive manufacturing aspects such as: the deposited powder layer, G-code input, heat source interaction, contact, bonding, thermal conduction and the accumulation of residual stresses and deformations.
Clinical characteristics and outcome of iatrogenic colonic perforation related to diagnostic vs. therapeutic colonoscopy
Authors: Cha Ra Ri , Kim Hee Jin , Lee Chang Min , Lee Jae Min , Lee Sang Soo , Cho Hyun Jin , Ha Chang Yoon , Kim Hyun Jin , Lee Ok-Jae 
Published: January 2022

AbstractAimIatrogenic colonic perforation (ICP) is a rare serious complication of colonoscopy, where standard treatment is controversial. This study aimed to characterize diagnostic ICP (DICP) compared to therapeutic ICP (TICP) and determine the possible indication of endoscopic repair.MethodsWe studied patients with ICP over 7 years starting in 2011. Their demographics and data regarding perforation, treatment, and outcome were investigated by retrospective review of medical records, and the diagnostic and therapeutic groups were compared.ResultsAmong 29,882 patients who underwent colonoscopy, ICP was identified in 28 (0.09%: diagnostic, 15/24,758, 0.06%; therapeutic, 13/5124, 0.25%). A total of 56 patients (33 DICP and 23 TICP) including 28 referred cases were analyzed. Mean age was 62.3 ± 11.4 years, and 24 were men. Perforations occurred mostly in the rectosigmoid region and half were detected during or immediately after colonoscopy. Endoscopic treatment was successful in 22 cases and 34 required surgery. Mortality occurred in 4 (7.1%).Compared to TICP, DICP was more prevalent in females and rectosigmoid region and more frequently detected immediately (allp < 0.05); DICP tended to occur in older patients, be larger and have better chance of endoscopic repair.Regardless of type of ICP, female predominance, smaller perforation, more frequent immediate detection, and shorter hospital stay (allp = 0.01) were found in the endoscopic repair group.ConclusionDICP was more frequent in the rectosigmoid area in older women and could be detected immediately. Immediate detection and small perforation size could be important factors for endoscopic repair. Careful attention and gentle manipulation should be required.
Post-marketing surveillance of the safety and effectiveness of naldemedine in the management of opioid-induced constipation in patients with cancer pain in Japan
Authors: Takata Keiko , Nakazawa Masami , Honda Keiichi , Hashimoto Sayo 
Published: January 2022

Abstract
Purpose
This prospective post-marketing surveillance (PMS) was designed to collect data on the safety and effectiveness of naldemedine in routine clinical practice in patients with opioid-induced constipation (OIC) and cancer pain in Japan and explore the characteristics of patients prone to diarrhea.

Methods
The enrolled patients received naldemedine (0.2 mg, once a day) orally for up to 12 weeks. In the safety analysis, adverse drug reactions (ADRs), including diarrhea as a special interest, were assessed. Effectiveness was evaluated, especially regarding the frequency and condition of bowel movement.

Results
In the safety analysis set (n = 1177), 145 ADRs occurred in 133 (11.30%) patients, and diarrhea was the most frequent event (n = 107, 9.09%). Most cases of diarrhea were non-serious (98.1%). Most ADRs were non-serious (93.8%), and they resolved within 2 weeks (75.9%). No patient characteristics influenced the risk of diarrhea development or aggravation. Both the frequency (75.0% and 83.2%) and condition of bowel movement (80.0% and 88.0%) were improved at 2 and 12 weeks, respectively in the effectiveness analysis set (n = 953). Frequency and condition of bowel movement were also improved in patients excluded (e.g., Eastern Cooperative Oncology Group performance status was ≥ 3) or with very small numbers (e.g., received weak opioid) in the clinical trials.

Conclusions
This PMS indicates that naldemedine is well tolerated and effective in patients of various backgrounds in routine clinical practice who have OIC and cancer pain.

Trial registration
UMIN000042851.
Feasibility of continuous epidural analgesia in patients with failed back surgery syndrome and spinal stenosis
Authors: Heck Vincent J. , Himpe Bastian , Kessler Paul , Almajali Asem , Prasse Tobias , Schmidt Sven , Rauschmann Michael 
Published: January 2022

Abstract
Purpose
The purpose of this study was to outline the feasibility of continuous epidural analgesia in the treatment of failed back surgery syndrome (FBSS) or spinal stenosis.

Methods
We queried our prospective collected institutional database to include all consecutive patients, who underwent continuous epidural analgesia with accompanying intensive physiotherapeutic exercise within a timeframe of 4 years. Patients suffered from FBSS or spinal stenosis; protocolled continuous epidural analgesia was planned for 4 days within the framework of an inpatient multimodal pain therapy concept. The instillation technique of the epidural catheter, the capability to attend in accompanying physiotherapy, and the peri-interventional complications were evaluated.

Results
153 patients with an average age of 57.4 years (± 11.9) were enrolled in this study. 105 patients suffered from FBSS and 48 patients had spinal stenosis. Overall, 148 patients (96.7%) reported the pain reduction and were able to perform daily intensified physiotherapeutic exercise. There were no serious adverse events, neither infection nor bleeding, no cardiopulmonary complication or permanent neurological deficits. The most common side effect was neurological impairment, such as numbness, dysesthesia, or weakness of the lower limbs with complete regression after flow rate adjustment. Patients with FBSS were more likely to develop dysesthesia (p = 0.007).

Conclusions
Continuous epidural analgesia is feasible in patients with FBSS or spinal stenosis. This treatment enables extensive physiotherapeutic treatment even in patients with severe pain conditions and can be considered as an alternative to epidural injections. An increased complication rate in comparison to short-term perioperative or perinatal application was not observed.
What can we learn from long-term studies on chronic low back pain? A scoping review
Authors: Dutmer Alisa L. , Soer Remko , Wolff André P. , Reneman Michiel F. , Coppes Maarten H. , Schiphorst Preuper Henrica R. 
Published: January 2022

AbstractPurposeA scoping review was conducted with the objective to identify and map the available evidence from long-term studies on chronic non-specific low back pain (LBP), to examine how these studies are conducted, and to address potential knowledge gaps.MethodWe searched MEDLINE and EMBASE up to march 2021, not restricted by date or language. Experimental and observational study types were included. Inclusion criteria were: participants between 18 and 65 years old with non-specific sub-acute or chronic LBP, minimum average follow-up of > 2 years, and studies had to report at least one of the following outcome measures: disability, quality of life, work participation, or health care utilization. Methodological quality was assessed using the Effective Public Health Practice Project quality assessment. Data were extracted, tabulated, and reported thematically.ResultsNinety studies met the inclusion criteria. Studies examined invasive treatments (72%), conservative (21%), or a comparison of both (7%). No natural cohorts were included. Methodological quality was weak (16% of studies), moderate (63%), or strong (21%) and generally improved after 2010. Disability (92%) and pain (86%) outcomes were most commonly reported, followed by work (25%), quality of life (15%), and health care utilization (4%). Most studies reported significant improvement at long-term follow-up (median 51 months, range 26 months–18 years). Only 10 (11%) studies took more than one measurement > 2 year after baseline.ConclusionPatients with persistent non-specific LBP seem to experience improvement in pain, disability and quality of life years after seeking treatment. However, it remains unclear what factors might have influenced these improvements, and whether they are treatment-related. Studies varied greatly in design, patient population, and methods of data collection. There is still little insight into the long-term natural course of LBP. Additionally, few studies perform repeated measurements during long-term follow-up or report on patient-centered outcomes other than pain or disability.
A novel in vitro periodontal pocket model to evaluate the effect of root surface instrumentation on biofilm-epithelial cell interactions
Authors: Lang Kiri N. , Sculean Anton , Eick Sigrun , Stähli Alexandra 
Published: January 2022

Abstract
Objective
To develop a novel in vitro periodontal pocket model for evaluating the effect of two different root surface instrumentation modalities on biofilm-epithelial cell interactions.

Materials and methods
An artificial periodontal pocket model was created using an impression material. Dentin discs were prepared and incubated for 3.5 days with a biofilm consisting of 12 bacterial strains. Then, the discs were inserted into the pocket model and instrumented for 10 s or 10 strokes either with ultrasonics (US) or hand instruments (HI). Subsequently, a glass slide coated with epithelial cells was placed in close vicinity to the discs. After incubation of the pocket model in a 5% CO2 atmosphere for 6 h, residual bacteria of the biofilm as well as bacteria adhering to or invaded into epithelial cells were determined using colony-forming unit (cfu) counts and real-time PCR. Further, as a parameter of the pro-inflammatory cell response, interleukin (IL)-8 expression was determined by ELISA.

Results
Compared to untreated control, HI reduced the cfu counts by 0.63 log10 (not significant) and US by 1.78 log10 (p = 0.005) with a significant difference between the treatment modalities favoring US (p = 0.048). By trend, lower detection levels of Tannerella forsythia were detected in the US group compared to HI. Concerning the interaction with epithelial cells, half of the control and the HI samples showed epithelial cells with attaching or invading bacteria, while US displayed bacteria only in two out of eight samples. In addition, US resulted in significantly lower IL-8 secretion by epithelial cells compared to the untreated control. Between HI and controls, no statistically significant difference in IL-8 secretion was found.

Conclusion
This newly developed in vitro model revealed in terms of biofilm-epithelial cell interaction after root surface instrumentation that compared to hand curettes, ultrasonic instrumentation appeared to be more effective in removing bacterial biofilm and in decreasing the inflammatory response of epithelium to biofilm.

Clinical relevance
Ultrasonic instrumentation might be more advantageous to reduce cellular inflammatory response than hand instruments.
An intentional cohesion call in male chimpanzees of Budongo Forest
Authors: Bouchard Alice , Zuberbühler Klaus 
Published: January 2022

AbstractMany social animals travel in cohesive groups but some species, including chimpanzees, form flexible fission–fusion systems where individuals have some control over group cohesion and proximity to others. Here, we explored how male chimpanzees of the Sonso community of Budongo Forest, Uganda, use communication signals during resting, a context where the likelihood of group fission is high due to forthcoming travel. We focused on a context-specific vocalisation, the ‘rest hoo’, to investigate its function and determine whether it is produced intentionally. We found that this call was typically given towards the end of typical silent resting bouts, i.e., the period when individuals need to decide whether to continue travelling after a brief stop-over or to start a prolonged resting bout. Subjects rested longer after producing ‘rest hoos’ and their resting time increased with the number of calls produced. They also rested longer if their calls were answered. Furthermore, focal subjects’ resting time was prolonged after hearing others’ ‘rest hoos’. Subjects called more when with top proximity partners and in small parties and rested longer if a top proximity partner called. We also found an interaction effect between rank and grooming activity, with high-ranking males with a high grooming index calling less frequently than other males, suggesting that vocal communication may serve as a cohesion strategy alternative to tactile-based bonding. We discuss these different patterns and conclude that chimpanzee ‘rest hoos’ meet key criteria for intentional signalling. We suggest that ‘rest hoos’ are produced to prolong resting bouts with desired partners, which may function to increase social cohesion.
Modelling in low-code development: a multi-vocal systematic review
Authors: Bucaioni Alessio , Cicchetti Antonio , Ciccozzi Federico 
Published: January 2022

AbstractIn 2014, a new software development approach started to get a foothold: low-code development. Already from its early days, practitioners in software engineering have been showing a rapidly growing interest in low-code development. In 2021 only, the revenue of low-code development technologies reached 13.8 billion USD. Moreover, the business success of low-code development has been sided by a growing interest from the software engineering research community. The model-driven engineering community has shown a particular interest in low-code development due to certain similarities between the two. In this article, we report on the planning, execution, and results of a multi-vocal systematic review on low-code development, with special focus to its relation to model-driven engineering. The review is intended to provide a structured and comprehensive snapshot of low-code development in itspeak of inflated expectationstechnology adoption phase. From an initial set of potentially relevant 720 peer-reviewed publications and 199 grey literature sources, we selected 58 primary studies, which we analysed according to a meticulous data extraction, analysis, and synthesis process. Based on our results, we tend to frame low-code development as a set of methods and/or tools in the context of a broader methodology, often being identified as model-driven engineering.
Confirmation of avian trichomonosis among wild birds in Ireland
Authors: Doyle Susan , Reilly Margaret , De Waal Theo , McMahon Barry J. 
Published: January 2022

AbstractTrichomonas gallinae is the causative protozoan parasite of avian trichomonosis, an infectious disease of wild bird species. Since an outbreak in the UK in 2005, avian trichomonosis has resulted in mass mortality events in greenfinch (Chloris chloris) and chaffinch (Fringillia coelebs) populations. Given its proximity to the UK, it is important to monitor the emergence of T. gallinae in Ireland. This study proposed to sample greenfinch, goldfinch (Carduelis carduelis) and feral pigeon (Columba livia domestica) in Ireland with the aim of culturing and isolating T. gallinae. The oral cavity of birds was sampled with an oropharyngeal swab, which was then inoculated into Biomed InPouch™ TF test kits. T. gallinae infection was diagnosed in 9 of 60 sampled birds (6 greenfinch and 3 feral pigeon), giving a total infection prevalence rate of 15% and providing evidence that avian trichomonosis is present among, but may not be limited to, greenfinch and feral pigeon populations in Ireland. It is also the first laboratory-based diagnosis of T. gallinae from live, free-living birds in Ireland to our knowledge. It is possible that avian trichomonosis is contributing to the recent decline in greenfinch populations in Ireland. Additionally, the presence of yeast cells in some positive samples did not appear to impede trichomonad growth, which may suggest a potential symbiotic relationship between T. gallinae and yeast species.
Flurbereinigung: Privatnützigkeit und Ökosystemleistungen
Authors: Möckel Stefan , Wolf André 
Published: January 2022

ZusammenfassungFlurbereinigung diente lange Zeit der Umgestaltung des ländlichen Raums hin zu intensiv genutzten
Agrar- und Forstlandschaften. Sie ist aufgrund der rechtlichen Festsetzungsmöglichkeiten aber auch
hervorragend geeignet, diese Landschaften nunmehr an den Klimawandel und die sonstigen ökologischen
Nachhaltigkeitserfordernisse anzupassen, da sie u.a. die Wiederherstellung von Landschaftselementen und
Habitaten für wildlebende Arten, die Schaffung guter Zustände bei Oberflächengewässern
und Grundwasserständen sowie die eigentumsrechtliche Arrondierung von Schutzgebieten ermöglicht.
Rechtlich müssen Flurbereinigungsverfahren allerdings vorrangig den Interessen der beteiligten Grundstückseigentümer
dienen (Privatnützigkeit), sofern nicht die Voraussetzungen für eine enteignende Unternehmensflurbereinigung
vorliegen. Maßnahmen des Umwelt- und Naturschutzes wurden bisher meist nicht als privatnützig
angesehen, was den Anwendungsbereich des Instruments einschränkte. Mit der Anerkennung und Identifizierung
von Ökosystemleistungen könnte sich jedoch der Anwendungsbereich deutlich erweitern, da viele
dieser Leistungen auch oder sogar in erster Linie den Grundstückseigentümern zugutekommen. Der
Aufsatz zeigt, in welchem Umfang die verschiedenen Verfahren des Flurbereinigungsrechts die Belange des
Umwelt- und Naturschutzes mit aufgreifen und inwieweit Maßnahmen zur Wiederherstellung von Ökosystemen
und ihrer Leistungen privatnützige, gemeinschaftliche Anlagen darstellen.