bluuebunny/crossref_metadata_2025_split huggingface dataset:
True FRET‐Based Sensing of pH via Separation of FRET and Photon Reabsorption
Authors: Corbella Bagot Conrad , Rappeport Eric , Das Ananda , Ba Tis Taleb , Park Wounjhang 
Published: May 2022

AbstractFörster resonance energy transfer (FRET)‐based devices have been extensively researched as potential biosensors due to their highly localized responsivity. In particular, dye‐conjugated upconverting nanoparticles (UCNPs) are among the most promising FRET‐based sensor candidates. UCNPs have a multi‐modal emission profile that allows for ratiometric sensing, and by conjugating a biosensitive dye to their surface, this profile can be used to measure localized variations in biological parameters. However, the complex nature of the UCNP energy profile as well as reabsorption of emitted photons must be taken into account in order to properly sense the target parameters. To the authors’ knowledge, no proposed UCNP‐based sensor has accurately taken care of these intricacies. In this article, the authors account for these complexities by creating a FRET‐based sensor that measures pH. This sensor utilizes Thulium (Tm3+)‐doped UCNPs and the fluorescent dye fluorescein isothiocyanate (FITC). It is first demonstrated that photon reabsorption is a serious issue for the 475 nm Tm3+ emission, thereby limiting its use in FRET‐based sensing. It is then shown that by taking the ratio of the 646 and 800 nm emissions rather than the more popular 475 nm one, it is possible to measure pH exclusively through FRET.
Metal‐Free C−H Functionalization via Diaryliodonium Salts with a Chemically Robust Dummy Ligand
Authors: Chen Yixuan , Gu Yuefei , Meng Huan , Shao Qianzhen , Xu Zhenchuang , Bao Wenjing , Gu Yucheng , Xue Xiao‐Song , Zhao Yanchuan 
Published: May 2022

AbstractA two‐step strategy for the transition‐metal‐free C−H functionalization of arenes using unsymmetrical iodonium salts as versatile synthetic linchpins is presented. The key to the success of this strategy is the identification of the 3,5‐dimethyl‐4‐isoxazolyl (DMIX) group as a superior dummy ligand, which enables not only site‐selective C−H functionalization to afford unsymmetrical iodonium salts, but also highly selective aryl transfer during the subsequent metal‐free coupling reaction. Both electron‐rich and moderately electron‐deficient arenes can be converted into the iodonium salts through C−H functionalization, allowing for diverse structural elaboration by metal‐free C−N, C−C, C−S, and C−O coupling.
Siliconoid Expansion by a Single Germanium Atom through Isolated Intermediates
Authors: Poitiers Nadine E. , Huch Volker , Morgenstern Bernd , Zimmer Michael , Scheschkewitz David 
Published: May 2022

AbstractThe growth of (semi‐)metal clusters is pivotal for nucleation processes in gaseous and condensed phases. We now report the isolation of intermediates during the expansion of a stable unsaturated silicon cluster (siliconoid) by a single germanium atom through a sequence of substitution, rearrangement and reduction. The reaction of ligato‐lithiated hexasilabenzpolarene LiSi6Tip5 (1Li⋅(thf)2, Tip=2,4,6‐triisopropylphenyl) with GeCl2⋅NHC (NHC=1,3‐diisopropyl‐4,5‐dimethylimidazol‐2‐ylidene) initially yields the product with exohedral germanium(II) functionality, which then inserts into an Si−Si bond of the Si6 scaffold. The concomitant transfer of the chloro functionality from germanium to an adjacent silicon preserves the electron‐precise nature of the formed endohedral germylene. Full incorporation of the germanium heteroatom to the Si6Ge cluster core is finally achieved either by reduction under loss of the coordinating NHC or directly by reaction of 1Li⋅(thf)2 with GeCl2⋅1,4‐dioxane.
Copper complex/polyoxometalate‐based tunable multi‐color film for energy storage
Authors: Chu Dongxue , Qu Xiaoshu , Zhang Shangfei , Zhang Jianrong , Liu Zefeng , Zhou Lili , Yang Yanyan 
Published: May 2022

AbstractPolyoxometalates (POMs) are considered as an attractive option for electrochromic energy storage (EES) system due to their unique electrochemical feature. Herein, a composite film NW/P2W17/Cu (phen)2 based on Dawson‐type monolacunary POMs K10P2W17O61 (abbreviated as P2W17) and copper complex [CuII (phen)2](NO3)2 (abbreviated as Cu (phen)2) formed on TiO2 nanowire (abbreviated as NW) array substrate was fabricated by hydrothermal process and layer‐by‐layer self‐assembly combination method. This rational designed film NW/P2W17/Cu (phen)2 exhibits excellent tunable multicolor electrochromic behavior and improved pseudocapacitive performance with large optical modulation (43.7% at 600 nm), fast switching time (tc = 2.9 s, tb = 9.5 s), and high volumetric capacitance (228.0 F cm−3 at 0.3 mA cm−2). In the process of charge and discharge, the composite film can realize the transformation from light green to orange, green, and finally to blue‐green color, successfully achieve the bridge between electrochromic behavior and energy storage, and monitor the level of stored energy by color change. This work showed great potential of POMs‐based electrode materials for integrated electrochromism and energy storage applications.
Assessing Water Use in WaterSense‐Labeled Homes and Quantifying the Savings
Authors: Schein Jonah , Bickmore Toby , Sovocool Kent 
Published: May 2022

Key TakeawaysAfter the local Water Smart Homes program ended in 2020, the US Environmental Protection Agency began the WaterSense‐labeled homes Version 2.0 program, launched as a pilot.The study evaluated indoor and outdoor metered water use of 160 WaterSense‐labeled homes in the Las Vegas metropolitan region.Metered data show that WaterSense‐labeled homes used a median of 44,000 gpy and exceeded their water savings goal.
Digitalisierung von Bestandsbauwerken mit KI
Authors: Reiterer Alexander , Merkle Dominik , Schmitt Annette 
Published: May 2022

AbstractDer Zustand eines Bauwerks ist dadurch gekennzeichnet, dass er sich mit zunehmendem Alter immer schneller verschlechtert. Eine vorbeugende Maßnahme gegen die Alterung ist umso erfolgreicher, je früher sie ergriffen wird. Um die Nutzung komplexer Bauwerke zu verlängern, sind umfassende Informationen zu einem möglichst frühen Zeitpunkt erforderlich. Auf dem Weg zu einer vorausschauenden Instandhaltung ist Grundlagenforschung zu den Methoden der Erfassung, Zusammenführung und Auswertung aller Geometrie‐, Material‐, Spannungs‐ und Alterungsdaten erforderlich. Die Digitalisierung im Sinne der Erzeugung eines Digitalen Zwillings erhält in diesem Zusammenhang eine völlig neue Bedeutung. Sie ermöglicht die Zusammenführung und Echtzeitauswertung aller für Betrieb und Instandhaltung erforderlichen Daten. Die Bauwirtschaft steht hier jedoch vor besonderen Herausforderungen. Bauwerke, insbesondere in der Verkehrsinfrastruktur, sind einzigartige Objekte. Sie zeichnen sich durch enorme Dimensionen aus und haben eine wesentlich längere Lebensdauer als andere technische Anlagen. Die Veränderungen im Sinne einer Zustandsverschlechterung sind sehr gering und daher kaum messbar. Die Basis eines umfassenden Zustandsmonitorings ist eine umfassende und komplette digitale Repräsentation des Bauwerks. Dies bedeutet eine Abbildung der kompletten äußeren und inneren Struktur in einer digitalen Form, inkl. Informationen zu Materialien, Schadstellen etc. Daraus lässt sich die Forschungsfrage ableiten, ob sich eine solche Abbildung effizient umsetzen lässt und in welcher Form und mit welchen Methoden dies möglich ist. Der vorliegende Beitrag gibt einen Überblick, wie die Umsetzung auch für komplexe Objekte gelingt und wie das generierte Modell mit semantischer Information angereichert werden kann.
Use of one‐to‐one psychotherapeutic interventions for people experiencing severe and multiple disadvantages: An evaluation of two regional pilot projects
Authors: Dowding Kerry , Murphy David , Everitt Grant , Tickle Anna 
Published: May 2022

AbstractBackgroundPeople with “severe and multiple disadvantages” (SMD) experience a combination of homelessness, mental health difficulties, alcohol or substance misuse and offending. Despite frequent and cyclical traumatic experiences being commonplace, people who experience SMD are often excluded from appropriate psychotherapeutic support.MethodsTwo pilot projects which aimed to work psychologically with people accessing SMD‐specific services were evaluated using a quantitative quasi‐experimental design.FindingsThose receiving psychotherapeutic support were more likely to engage in positive developmental contact with external support services, and less likely to require reactive, negative contacts. They were also more likely to stay engaged with the programme’s internal support services for longer than those not receiving psychotherapeutic support.Implications for practiceThe findings are particularly noteworthy for future commissioning practices, as they indicate that the benefits of such interventions could be quantifiable for the wider support system.ConclusionsThis research adds to a growing body of evidence that psychotherapeutic interventions can improve outcomes for people who experience SMD.
Anti‐Inflammatory Lignans from the Roots of Asarum heterotropoides var. mandshuricum and Their Mechanism of Action
Authors: Thanh Le Thi , Tuan Ha Manh , Han Kang‐Hyun , Kim Yong‐Bum , Ah Kim Jeong , Sun Min Byung 
Published: May 2022

AbstractBioassay‐guided fractionation of Asarum heterotropoides var. mandshuricum F. Maekawa (Aristolochiaceae) root extract led to the isolation and characterization of one new ferulic acid glucose ester (1) and nine known lignans (2‐10). Their structures were elucidated using extensive spectroscopic methods, including 1D and 2D NMR, and MS spectra. The anti‐inflammatory effects of the isolated compounds were investigated via their inhibition against nitric oxide (NO) production in lipopolysaccharide (LPS)‐stimulated RAW264.7 mouse macrophage cells. Among them, compound 7 ((1R,2S,5R,6R)‐5′‐O‐methylpluviatilol) showed the most effective inhibitory activity against NO production and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2) protein in an exceedingly dose‐dependent manner. In addition, further study revealed that the mechanism of anti‐inflammatory activity of the most active lignan (7) might be associated with the inhibition of extracellular‐signal‐regulated kinase (ERK) and nuclear factor kappa B (NF‐κB) phosphorylation.
Quantifying Escherichia coli contamination in milling equipment during lab‐scale milling operations
Authors: Rivera Jared , Pulivarthi Manoj K. , Shivaprasad DP , Phebus Randall , Aldrich Charles G. , Siliveru Kaliramesh 
Published: May 2022

AbstractBackground and ObjectivesCross‐contamination of foodborne pathogens from equipment to food is a food safety concern. The objective of this study was to quantify the amount of Escherichia coli contamination in mill equipment after milling inoculated and noninoculated wheat in single milling run.FindingsMill equipment used for milling inoculated kernels contained substantial amounts of E. coli. The hoppers, feeders, rollers, and break system sifter surfaces had higher E. coli counts compared to other surfaces sampled (2.0–3.6 log CFU/100 cm2). Surfaces from the break system of the mill had higher counts in general compared to the sizing/reduction system. The majority of equipment surfaces had nonquantifiable E. coli counts after milling noninoculated kernels indicating cross‐contamination of E. coli from contaminated equipment to mill fractions.ConclusionsThe findings indicate that E. coli could easily transfer from wheat kernels into milling equipment and vice‐versa. This demonstrates that mill equipment is a viable route for E. coli contamination during milling and that cleaning and sanitation practices could be focused on the surfaces mentioned in this study to reduce cross‐contamination risk.Significance and NoveltyThe findings from this study could help determine surfaces where microbial contamination could accumulate, which could improve mill cleaning and sanitation practices.
1‐(Azidomethyl)‐5H‐Tetrazole: A Powerful New Ligand for Highly Energetic Coordination Compounds
Authors: Kofen Moritz , Lommel Marcus , Wurzenberger Maximilian H. H. , Klapötke Thomas M. , Stierstorfer Jörg 
Published: May 2022

AbstractHighly energetic 1‐(azidomethyl)‐5H‐tetrazole (AzMT, 3) has been synthesized and characterized. This completes the series of 1‐(azidoalkyl)‐5H‐tetrazoles represented by 1‐(azidoethyl)‐5H‐tetrazole (AET) and 1‐(azidopropyl)‐5H‐tetrazole (APT). AzMT was thoroughly analyzed by single‐crystal X‐ray diffraction experiments, elemental analysis, IR spectroscopy and multinuclear (1H, 13C, 14N, 15N) NMR measurements. Several energetic coordination compounds (ECCs) of 3d metals (Mn, Fe, Cu, Zn) and silver in combination with anions such as (per)chlorate, mono‐ and dihydroxy‐trinitrophenolate were prepared, giving insight into the coordination behavior of AzMT as a ligand. The synthesized ECCs were also analyzed by X‐ray diffraction experiments, elemental analysis, and IR spectroscopy. Differential thermal analysis for all compounds was conducted, and the sensitivity towards external stimuli (impact, friction, and ESD) was measured. Due to the high enthalpy of formation of AzMT (+654.5 kJ mol−1), some of the resulting coordination compounds are extremely sensitive, yet are able to undergo deflagration‐to‐detonation transition (DDT) and initiate pentaerythritol tetranitrate (PETN). Therefore, they are to be ranked as primary explosives.
Decongestive treatment adjustments in heart failure patients remotely monitored with a multiparametric implantable defibrillators algorithm
Authors: Guerra Federico , D'Onofrio Antonio , De Ruvo Ermenegildo , Manzo Michele , Santini Luca , Giubilato Giovanna , La Greca Carmelo , Petracci Barbara , Stronati Giulia , Bianchi Valter , Martino Annamaria , Franculli Fabio , Compagnucci Paolo , Campari Monica , Valsecchi Sergio , Dello Russo Antonio 
Published: May 2022

AbstractAimsHeartLogic algorithm combines data from multiple implantable defibrillators (ICD)‐based sensors to predict impending heart failure (HF) decompensation. A treatment protocol to manage algorithm alerts is not yet known, although decongestive treatment adjustments are the most frequent alert‐triggered actions reported in clinical practice. We describe the implementation of HeartLogic for remote monitoring of HF patients, and we evaluate the approach to diuretic dosing and timing of the intervention in patients with device alerts.MethodsThe algorithm was activated in 229 ICD patients at eight centers. The median follow‐up was 17 months (25th–75th percentile: 11–24). Remote data reviews and patient phone contacts were undertaken at the time of HeartLogic alerts, to assess the patient's status and to prevent HF worsening. We analyzed alert‐triggered augmented HF treatments, consisting of isolated increases in diuretics dosage.ResultsWe reported 242 alerts (0.8 alerts/patient‐year) in 123 patients, 137 (56%) alerts triggered clinical actions to treat HF. The HeartLogic index decreased after the 56 actions consisting of diuretics increase. Specifically, alerts resolved more quickly when the increases in dosing of diuretics were early rather than late: 28 days versus 62 days, p < .001. The need of hospitalization for further treatments to resolve the alert condition was associated with higher HeartLogic index values on the day of the diuretics increase (odds ratio: 1.11, 95% CI: 1.02–1.20, p = .013) and with late interventions (odds ratio: 5.11, 95% CI: 1.09–24.48, p = .041). No complications were reported after drug adjustments.ConclusionsDecongestive treatment adjustments triggered by alerts seem safe and effective. The early use of decongestive treatment and the use of high doses of diuretics seem to be associated with more favorable outcomes.
Evaluation of electrophysiological characteristics and ventricular synchrony: An intrapatient‐controlled study during His‐Purkinje conduction system pacing versus right ventricular pacing
Authors: Chen Xueying , Zhou Xiaolan , Wang Yanan , Jin Qinchun , Chen Yufei , Wang Jingfeng , Qin Shengmei , Bai Jin , Wang Wei , Liang Yixiu , Chen Haiyan , Su Yangang , Ge Junbo 
Published: May 2022

AbstractObjectives to BackgroundTo compare electromechanical ventricular synchrony when pacing from different sites, including right ventricular apex pacing (RVAP), right ventricular septum pacing (RVSP), His bundle pacing (HBP), left bundle branch pacing (LBBP), and RVSP during unipolar pacing from the ring electrode of LBBP lead (RVSPring) in each patient and evaluate the correlations between electrophysiological characteristics and ventricular synchrony.MethodsTwenty patients with complete atrioventricular block indicated for dual‐chamber pacemaker implantation were included in the study. Unipolar pacing at different sites, including RVAP, RVSP, HBP, LBBP, and RVSPring, was successively performed in each patient. The pacing characteristics and echocardiogram parameters were collected and compared among intrinsic rhythm and pacing at different sites.ResultsSimilar to HBP (114.84 ± 18.67 ms), narrower paced QRSd was found in LBBP (116.15 ± 11.60 ms) as compared to RVSPring (135.11 ± 13.68 ms), RVSP (141.65 ± 14.26 ms), and RVAP (160.15 ± 19.35 ms) (p < .001). LBBP showed comparable pacing parameters to RVAP or RVSP and was significantly better than HBP, with maintained cardiac function. TS‐12‐SD was significantly improved in LBBP (41.80 ± 20.97 ms) than RVAP (69.70 ± 32.42 ms, p = .003) and RVSP (63.30.56 ± 32.53 ms, p = .018) but similar to HBP (51.50 ± 25.67 ms, p = .283) or RVSPring (57.80 ± 25.65 ms, p = .198). Among these pacing strategies, negative values of interventricular mechanical delay (IVMD) were only identified in LBBP (−19.25 ± 18.43 ms), significantly different from RVAP (35.00 ± 30.72 ms), RVSP (22.85 ± 22.05 ms), HBP (5.20 ± 18.64 ms), and RVSPring (16.00 ± 26.76 ms (all p < .05). Using Pearson's analysis, Sti‐LVAT was positively correlated with QRS duration, IVMD, TS‐12‐SD, LVEDV, and LVESV, while a negative relationship could be observed for left ventricular ejection fraction.ConclusionsHis‐Purkinje conduction system pacing (HPCSP) achieved better electrical and mechanical synchrony than conventional RV pacing. For interventricular synchrony, only LBBP initiated earlier LV activation than RV, in accordance with the right bundle branch block (RBBB) pattern of paced QRS during LBBP. Sti‐LVAT might be a good parameter correlating with LV systolic function and mechanical synchrony.
Inhibitors of the ubiquitin proteasome system block myofibril assembly in cardiomyocytes derived from chick embryos and human pluripotent stem cells
Authors: Wang Jushuo , Fan Yingli , Wang Chenyan , Dube Syamalima , Poiesz Bernard J. , Dube Dipak K. , Ma Zhen , Sanger Jean M. , Sanger Joseph W. 
Published: May 2022

AbstractDetails of sarcomeric protein assembly during de novo myofibril formation closely resemble myofibrillogenesis in skeletal and cardiac myocytes in birds, rodents, and zebrafish. The arrangement of proteins during myofibrillogenesis follows a three‐step process: beginning with premyofibrils, followed by nascent myofibrils, and concluding with mature myofibrils (reviewed in Sanger et al., 2017). Assembly and maintenance of myofibrils in living muscle cells. In: Handbook of experimental pharmacology, 2017 [pp. 39–75]. Our aim is to determine if the same pathway is followed in human cardiomyocytes derived from human inducible pluripotent stem cells. We found that the human cardiomyocytes developed patterns of protein organization identical to the three‐step series seen in the model organisms cited above. Further experiments showed that myofibril assembly can be blocked at the nascent myofibril by five different inhibitors of the Ubiquitin Proteasome System (UPS) stage in both avian and human cardiomyocytes. With the exception of Carfilzomib, removal of the UPS inhibitors allows nascent myofibrils to proceed to mature myofibrils. Some proteasomal inhibitors, such as Bortezomib and Carfilzomib, used to treat multiple myeloma patients, have off‐target effects of damage to hearts in three to 6 % of these patients. These cardiovascular adverse events may result from prevention of mature myofibril formation in the cardiomyocytes. In summary, our results support a common three‐step model for the formation of myofibrils ranging from avian to human cardiomyocytes. The Ubiquitin Proteasome System is required for progression from nascent myofibrils to mature myofibrils. Our experiments suggest a possible explanation for the cardiac and skeletal muscle off‐target effects reported in multiple myeloma patients treated with proteasome inhibitors.
Predictors of primary prevention implantable cardioverter‐defibrillator use in heart failure with reduced ejection fraction: impact of the predicted risk of sudden cardiac death and all‐cause mortality
Authors: Schrage Benedikt , Lund Lars H. , Benson Lina , Dahlström Ulf , Shadman Ramin , Linde Cecilia , Braunschweig Frieder , Levy Wayne C. , Savarese Gianluigi 
Published: May 2022

AimsUse of implantable cardioverter‐defibrillators (ICD) for primary prevention of sudden cardiac death (SCD) in heart failure with reduced ejection fraction (HFrEF) is limited. We aimed to investigate barriers to ICD use in HFrEF while considering the predicted risk of mortality and SCD.Method and resultsPatients from the SwedeHF registered in 2011–2018 and with an indication for primary prevention ICD were analysed. The Seattle Proportional Risk and Seattle Heart Failure Models were used to predict the proportional SCD and all‐cause mortality risk, respectively. A multivariable logistic regression model was fitted to identify independent predictors of ICD use/non‐use; Cox regression models to evaluate the interaction between predicted SCD/mortality risk and ICD use for mortality. Of 13 475 patients, only 15.5% had an ICD. Those with higher predicted proportional SCD risk (>45%) had an ∼80% higher likelihood to have an ICD. Other predictors of non‐use were follow‐up in primary versus specialty care, higher comorbidity burden and lower socioeconomic status. ICD use was associated with lower mortality only in patients with higher predicted SCD and lower mortality risk (34% and 37% relative risk reduction for 3‐year all‐cause and cardiovascular mortality, respectively). In this subgroup of patients, underuse of ICD was 81.8%.ConclusionIn a contemporary registry, only 15.5% of patients with an indication for primary prevention ICD received the device. While a high predicted proportional SCD risk was appropriately linked to ICD use, the lack of specialized follow‐up, higher comorbidity burden, and lower socioeconomic status were major unjustified impediments to implementation. Our findings suggest areas for improving ICD use for primary prevention of SCD in clinical practice.