bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Recombinant Capripoxvirus Expressing the F Protein of Peste des Petits Ruminants Virus and the P12A3C of Foot-and-mouth Disease Virus
Authors: Li Jidong , Wang Jianlin , Guo Yanan , Gong Zhenxing , Cai Xuepeng 
Published: August 2022

Abstract
Background: Peste des petits ruminants (PPR), foot-and-mouth disease (FMD) and goat pox (GP) are three important infectious diseases that infect goats, sheep and other small ruminants. It is well-known that the prevention of three diseases rely mainly on their individual vaccines. However, the vaccines have a variety of different disadvantages, such as short duration of immunity, increasing the number of vaccinations, and poor thermal stability. The purpose of this study is to construct a recombinant goat pox virus (rGPV) capable of expressing the F gene of PPRV and the P12A3C gene of FMDV as a live vector vaccine.Results: The IRES, FMDV P12A3C and PPRV F genes into the multi-cloning site of the universal transfer plasmid pTKfpgigp to construct a recombinant transfer plasmid pTKfpgigpFiP12A3C, and transfected GPV-infected lamb testis (LT) cells with liposomes and produced by homologous recombination Recombinant GPV (rGPV/PPRVF-FMDVP12A3C, rGPV). The rGPV was screened and purified by green florescence protein (GFP) and xanthine-guanine-phosphoribosyltransferase gene (gpt) of Escherichia coli as selective markers, and the expression of rGPV in LT cells was detected by RT-PCR and immunofluorescence techniques. The results showed that the virus strain rGPV/PPRVF-FMDVP12A3C containing FMDV P12A3C and PPRV F genes was obtained. The exogenous genes FMDV P12A3C and PPRV F contained in rGPV were normally transcribed and translated in LT cells, and the expression products could specifically react with PPRV and FMDV antiserum. Then, the rGPV was intradermally inoculated with goats, the animal experiments showed that rGPV/PPRVF-FMDVP12A3C could induce high levels of specific antibodies against GPV, PPRV and FMDV. Conclusions: The constructed rGPV induced high levels of specific antibodies against GPV, PPRV and FMDV. The study provides a reference for " one vaccine with multiple uses " of GPV live vector vaccine.
Implementing a text message-based intervention to support type 2 diabetes medication adherence in primary care: A qualitative study with general practice staff
Authors: Sharry Jenny Mc , Butler Karen , Bartlett Yvonne Kiera , Newhouse Nikki , Farmer Andrew , French David P. , Kenning Cassandra , Locock Louise , Rea Rustam , Williams Veronika 
Published: August 2022

Abstract
Background: The Support through Mobile Messaging and digital health Technology for Diabetes (SuMMiT-D) project has developed, and is evaluating, a mobile phone-based intervention delivering brief messages targeting identified behaviour change techniques promoting medication use to people with type 2 diabetes in general practice. The present study aimed to inform refinement and future implementation of the SuMMiT-D intervention by investigating general practice staff perceptions of how a text message-based intervention to support medication adherence should be implemented within current and future diabetes care.Methods: Seven focus groups and five interviews were conducted with 46 general practice staff (including GPs, nurses, healthcare assistants, receptionists and linked pharmacists) with a potential role in the implementation of a text message-based intervention for people with type 2 diabetes. Interviews and focus groups were audio-recorded, transcribed and analysed using an inductive thematic analysis approach.Results:Five themes were developed. One theme ‘The potential of technology as a patient ally’ described a need for diabetes support and the potential of technology to support medication use. Two themes outlined challenges to implementation, ‘Limited resources and assigning responsibility’ and ‘Treating the patient; more than diabetes medication adherence’. The final two themes described recommendations to support implementation, ‘Selling the intervention: what do general practice staff need to see?’ and ‘Fitting the mould; complementing current service delivery’. Conclusions:Staff see the potential for a text message-based support intervention to address unmet needs and to enhance care for people with diabetes. Digital interventions, such as SuMMiT-D, need to be compatible with existing systems, demonstrate measurable benefits, be incentivised and be quick and easy for staff to engage with. Interventions also need to be perceived to address general practice priorities, such as taking a holistic approach to care and having multi-cultural reach and relevance. Findings from this study are being combined with parallel work with people with type 2 diabetes to ensure stakeholder views inform further refinement and implementation of the SuMMiT-D intervention.
Afatinib plus osimertinib in the treatment of osimertinib-resistant non-small cell lung carcinoma: a phase I clinical trial
Authors: Miura Satoru , Koh Yasuhiro , Azuma Koichi , Yoshioka Hiroshige , Koyama Kenichi , Teraoka Shunsuke , Ishii Hidenobu , Kibata Kayoko , Ozawa Yuichi , Tokito Takaaki , Oyanagi Jun , Shimokawa Toshio , Kurata Takayasu , Yamamoto Nobuyuki , Tanaka Hiroshi 
Published: August 2022

Abstract
Background
Conquering acquired resistance to osimertinib remains a major challenge in treating patients with epidermal growth factor receptor (EGFR) mutation-positive non-small-cell lung cancer (NSCLC). Thus, we aimed to determine the safety and efficacy of combination treatment with osimertinib and afatinib for patients with acquired resistance to osimertinib.
Methods
This open-label phase I study was a feasibility study of the combination of afatinib and osimertinib for patients with advanced EGFR-positive NSCLC who had progressive disease after receiving osimertinib. The primary endpoint was to determine the maximum tolerated dose (MTD). We enrolled patients who received afatinib at three different dose levels (level 1, 20 mg; level 2, 30 mg; level 3, 40 mg) combined with osimertinib at a standard dose of 80 mg once per day.
Results
Thirteen patients were enrolled in this study. The MTD was defined as 30 mg afatinib when combined with daily oral administration of osimertinib (80 mg). The most frequent adverse events were diarrhea (76.9%), anemia (76.9%), and rash (69.2%). Considering the toxicity profiles during all treatment periods, the recommended oral dose of afatinib was determined as 20 mg daily, with an osimertinib dose of 80 mg. For all evaluable patients (n = 12), the response rate was 7.7% and disease-control rate was 46.2%.
Conclusion
Combination therapy with osimertinib and afatinib was tolerable; however, the synergistic effect of afatinib with osimertinib may be limited in osimertinib-resistant patients.
Trial registration:
Japan Registry of Clinical Trials ID: jRCTs051180008, registered date: 08/11/2018.
MicroRNAs-26 and related osteogenic target genes could play pivotal roles in Photobiomodulation and adipose-derived stem cells-based healing of critical size foot defects in the rat model
Authors: Mohebbi Hanieh , Siasi Elham , Khosravipour Armin , Asghari Mohammadali , Amini Abdollah , Mostafavinia Atarodalsadat , Bayat Mohammad 
Published: August 2022

Abstract
Background Photobiomodulation therapy (PBMT) and human adipose-derived stem cells (hADSs) treatment are relatively invasive methods for treating bone defects. Specific and oriented cellular and molecular functions can be induced by applying an appropriate type of PBMT and ADSs. In this study, we aimed to explore the role of MicroRNAs pathways in the PBMT, hADS, and PBM+hADS based on promoting critical size fracture healing.MethodsA critical size femoral defect (CSFD) is induced in the right and left femoral bones of 24 rats. Then a human demineralized bone matrix scaffold (hDBMS) was engrafted into the CSFDs of all rats. Experiments are performed on 4 groups (12 rats per group): (1) Control (hDBMS); (2) hDBMS+hADS, hADS was engrafted into CSFDs; (3) hDBMS+PBM, the CSFD was exposed to PBM(810 nm wavelength, 1.2 J/cm2 energy density); (4) hDBMS+(hADS+PBM), hADSs were implanted into CSFD then were exposed to PBM. At 42 days after CSFD induction, the rats were killed and, the left and right CSFDs were removed for the mechanical compression tests, and molecular and cellular studies, respectively.ResultsThe results indicate that miRNA-26a, BMP, SMAD, RUNX, and OSTREX were more expressed in the treated groups than in the control group. Furthermore, the biomechanical and histological properties of CSFDs in treated groups were better than the control group. The correlation tests revealed a positive relationship between microRNA and improving biomechanical and cellular parameters of CSFDs in the rat model.ConclusionWe concluded that the MicroRNA-26 signaling pathway plays a significant role in the hADS, PBM, and hADS+PBM-based healing of CSFDs in rats.
The declining trend in HIV prevalence from population-based surveys in Cameroon between 2004 and 2018: myth or reality in the Universal Test and Treat era?
Authors: Bekolo CE , Kouanfack C , Ateudjieu J , Bechem ET , Ndeso SA , Tendongfor N , Nsagha DS , Choukem SP 
Published: August 2022

Abstract
Background
HIV remains an epidemic of major public health importance in Cameroon but a decline in HIV prevalence has been observed according to population-based surveys conducted in 2004, 2011 and 2018. We sought to review current evidence for declining HIV prevalence despite increasing survival owing to ‘universal test and treat’ and to explore the reason for the decrease, particularly the role of behavioural change.
Methods
We conducted a secondary analysis using HIV prevalence, behavioural and social determinants data of the Demographic and Health Survey Program databases. Trend lines were fitted to data that were available for a minimum of three points in time during the 1991–2018 period. Regression coefficients, associated p-values and 95% confidence intervals were obtained using Microsoft Excel software.
Results
Overall adult HIV prevalence decreased significantly from 5.4% (95%CI: 4.8-6.0) in 2004 to 4.3% (95%CI: 3.8–4.8) in 2011 and further down to 2.7% (95%CI: 2.3–3.1) in 2018 at a rate of about 1.4% every septennium (β = -1.4, R² = 0.98, p = 0.03). Yet, the number of persons surviving with HIV increased from about 0.05 million in 1991 to 0.5 million in 2018 corresponding to an increase in access to antiretroviral therapy from less than 10% to universal coverage of 80% respectively. Concurrent reductions in risky sexual behaviours were observed: delayed sexual debut by one year, decreased sexual violence by 7%, decreased polygamous unions by 16%, decreased multiple sexual partners by 15.3% and increased condom use by 26.3%.
Conclusion
The observed decline in HIV prevalence is statistically valid and reflects the observed decline in risky sexual behaviour that need to be sustained by the National HIV programme. Though universal access to ART has increased the number of persons surviving with HIV, this has not led to an increased prevalence of HIV in a setting with a rising population.
Primary sternal chondrosarcoma local recurrence; tumor manipulation or self-seeding: When there's smoke, there's fire
Authors: Abdallah Farah A. , jalil Riad Abdel 
Published: August 2022

Abstract
Background: Primary sternal chondrosarcoma, although rare, is the most common malignant tumor of the sternum. Adequate surgical excision is the mainstay of treatment, which may compromise the chest wall stability and obligate for reconstruction. Local recurrence is common and increases the risk of distant metastasis. Case presentation: We report a rare case of a 60-year-old male patient diagnosed with primary sternum chondrosarcoma successfully resected as primary treatment with negative margins accompanied by two local recurrences at 11 months and 37 months from the original surgery which were managed with local resection and adjuvant radiation Conclusion: seeding theories has been increasingly reported recently including tumor biopsy seeding, self-seeding tumors and tumor manipulation seeding, our patient developed two local recurrences despite resection with free margins and no distant metastasis raises the concern for seeding.
Safety and genetic stability of African swine fever virus vaccine candidate “ASFV-G-∆MGF” in an in vivo “reversion to virulence” study
Authors: Blome Sandra , Deutschmann Paul , Forth Jan-Hendrik , Sehl-Ewert Julia , Carrau Tessa , Viaplana Elisenda , Mancera Jose , Urniza Alicia , Beer Martin 
Published: August 2022

Abstract
African swine fever (ASF) has gained panzootic dimensions and commercial vaccines are still unavailable. Recently, a series of live attenuated vaccines has raised hope for an efficacious and safe vaccine, among them “ASFV-G-∆MGF”. We tested the latter in a in vivo reversion to virulence study in accordance with International Cooperation on Harmonisation of Technical Requirements for Registration of Veterinary Medicinal Products guidelines. Upon forced animal passaging, a virus variant emerged that was associated with transient fever and an increased replication and shedding. However, all animals were healthy upon completion of the study and reversion to significant virulence was not observed. The genomic changes involved deletions and reorganizations in the terminal regions of the genome. While our study underscores that in-depth safety characterization is needed for live ASF vaccines, one should still conduct a thorough benefit risk analysis considering all safety and efficacy aspects when assessing their use in disease control.
DDX43 mRNA expression and protein levels in relation to clinicopathological profile of breast cancer
Authors: Amer Noha , Khairat Rabab , Hammad Amal M , Kamel Mahmoud M 
Published: August 2022

Abstract
Background: Breast cancer (BC) is the most often diagnosed cancer in women globally. To meet the increased overall protein synthesis and for translation of particular pro-oncogenic mRNAs in order to survive, cancer cells appear to rely heavily on RNA helicases. DDX43 is one of DEAD- box RNA helicase family members. But, the relationship between clinicopathological, prognostic significance, in different BC subtypes and DDX43 expression remains unclear. Our aim therefore is to assess the clinicopathological and prognostic significance in relation to DDX43 protein and mRNA expression. Materials and Methods: A total of 80 females newly diagnosed with BC and 20 control females, that were age matched, were recruited for this study. DDX43 protein levels were measured by ELISA technique. We used a real-time polymerase chain reaction quantification (real-time PCR) to measure the levels of DDX43 mRNA expression. Levels of DDX43 protein and mRNA expression within BC patients were compared to those of control subjects and correlated with clinicopathological data. Results: The mean normalized serum levels of DDX43 protein were slightly higher in control than in both benign and malignant groups, but this result was non-significant. The mean normalized level of DDX43 mRNA expression was higher in control than in both benign and malignant cases, although the results were not statistically significant and marginally significant respectively. Moreover, the mean normalized level of DDX43 mRNA expression was significantly higher in benign than in malignant cases. In malignant cases, low DDX43 protein expression was linked to higher nuclear grade and invasive duct carcinoma (IDC), whereas high mRNA expression was linked to a poor prognosis.Conclusion: Our study explored DDX43 as a cancer marker in human breast cancer. It has the potential to be used in clinical settings as a disease progression marker.
Experimental demonstration of multiple Fano resonances appearing due to the interaction of plasmonic mode with waveguide modes in a mirrored array of split-ring resonators
Authors: Kamarauskas Andrius , Seliuta Dalius , Šlekas Gediminas , Sadauskas Modestas , Kvietkauskas Evaldas , Trusovas Romualdas , Ratautas Karolis , Kancleris Žilvinas 
Published: August 2022

Abstract
This work demonstrates the first experimental observation of multiple Fano resonances in the terahertz range in a system based on an array of mirror-symmetric split-ring resonators deposited on low-loss and low-refractive index polytetrafluoroethylene (PTFE) substrate. For the first time, selective surface activation induced by laser technology has been used to deposit a copper layer on a PTFE substrate with the further application of standard mask lithography for metasurface manufacturing.
WHY STOP? Quantifying Cognitive-Behavioural factors that influence the impact of PCR-POCT results on antibiotic cessation in ICU
Authors: Singh Suveer , Nurek Martine , Mason Sonia , Moore Luke , Mughal Nabeela , Vizcaychipi Marcela , Consortium WHY STOP 
Published: August 2022

Abstract
INTRODUCTION: Rapid Point of Care Tests for infection (POCT) do not consistently improve antibiotic stewardship (ASP) of suspected ICU infection. We measured 1) the effect of a negative PCR-POCT on antibiotic stop decisions, and 2) clinico-behavioural factors that prevent stopping.METHODS: Vignettes of antibiotic treated respiratory infection, with 4 distinct trajectories were presented to ICU clinicians: overall improvement, clinical improvement/biological worsening, clinical worsening/biological improvement, overall worsening. Initial and post PCR-POCT antibiotic decisions (stop or continue) /confidence levels were recorded. The PCR-POCT offer was voluntary but always presented and negative. Linear regression determined association of their final decision with influencing factors.RESULTS: Seventy clinicians responded. A negative PCR-POCT increased stop decisions in all scenarios (p<0.001) except improvement (already high); especially in discordant clin worse(49% pre-POCT vs 74% post-POCT). Inclination to stop was reduced by an ambiguous/worsening trajectory(p=0.015), initial confidence to continue(p<0.001), and involuntary receipt of POCT(p<0.001), not clinician experience or risk averseness. CONCLUSIONS: Negative PCR-POCT increases the inclination to stop antibiotics, particularly in ambiguous/worsening trajectories of ICU infection. Clinician intuition to continue and disinterest in POCT reduce its influence to stop. Highlighting and quantifying the predictive impact of behavioural-trajectorial factors can improve antibiotic stewardship and study design in ICU related infection.
Assessment of Condylar Positional Changes in Severe Skeletal Class II Malocclusion after Surgical-Orthodontic Treatment
Authors: Yang Wen , Chen Yanbin , Li Jiaxuan , Jiang Nan 
Published: August 2022

Abstract
Objectives
This study aimed to determine the positional changes in the condyle in the temporomandibular joint (TMJ) of severe skeletal Class II malocclusion patients treated with surgical- orthodontics.
Materials and Methods
The measurements of TMJ space in 97 severe skeletal Class II malocclusion patients (20 males, 77 females, mean age, 24.8 years, mean ANB = 7.41°) were assessed using limited cone-beam computed tomography (LCBCT) images acquired before orthodontics (T0) and 12 months after surgery (T1). 3D remodelling of the TMJ and measurements of the anterior space (AS), superior space (SS), and posterior space (PS) were performed to determine the position of the condyle for each joint. All data were analysed by t test, correlation analysis and Pearson correlation coefficient.
Results
The mean AS, SS, and PS values after the therapy changed from 1.684 mm to 1.680 mm (0.24%), 3.086 mm to 2.748 mm (10.968%), and 2.873 mm to 2.155 mm (24.985%), respectively. The decreases in SS and PS were statistically significant. Positive correlations were found in the mean AS, SS, and PS values between the right and left sides.
Conclusions
The combination of orthodontic and surgical treatment makes the condyle move counter-clockwise in the TMJ in severe skeletal Class II patients.
Clinical Relevance:
Studies of temporomandibular joint (TMJ) intervals changes in patients with severe skeletal class II after sagittate split ramus osteotomy (SSRO) are limited. The postoperative joint remodelling, resorption and related complications remain unstudied.
Office Blood Pressure versus Ambulatory Blood Pressure Measurement in Childhood Obesity
Authors: Heijden Laila B , Groothoff Jaap W. , Feskens Edith JM , Janse Arieke J 
Published: August 2022

Abstract
Background
The prevalence of obesity-related co-morbidities is rising parallel to the childhood obesity epidemic. High blood pressure (BP), as one of these co-morbidities, is detected nowadays at increasingly younger ages. The diagnosis of elevated BP and hypertension, especially in the childhood population, presents a challenge to clinicians. The added value of ambulatory blood pressure measurement (ABPM) in relation to office blood pressure (OBP) measurements in obese children is unclear. Furthermore, it is unknown how many overweight and obese children have an abnormal ABPM pattern. In this study we evaluated ABPM patterns in a population of overweight and obese children and adolescents, and compared these patterns with regular OBP measurements.
Methods
In this cross-sectional study in children and adolescents with overweight or obesity aged 4–17 years who were referred to secondary pediatric obesity care in a large general hospital in The Netherlands, OBP was measured during a regular outpatient clinic visit. Additionally, all participants underwent a 24-uur ABPM on a regular week-day. Outcome measures were OBP, mean ambulatory BP, BP load, ambulatory BP pattern (normal BP, white-coat hypertension, elevated BP, masked hypertension, ambulatory hypertension), and BP dipping.
Results
We included 82 children aged 4–17 years. They had a mean BMI Z-score of 3.3 (standard deviation 0.6). Using ABPM, 54.9% of the children were normotensive (95% confidence interval 44.1–65.2), 26.8% had elevated BP, 9.8% ambulatory hypertension, 3.7% masked hypertension, and 4.9% white-coat hypertension. An isolated night-time BP load > 25% was detected in almost a quarter of the children. Forty percent of the participants lacked physiologic nocturnal systolic BP dipping. In the group of children with normal OBP, 22.2% turned out to have either elevated BP or masked hypertension on ABPM.
Conclusions
In this study a high prevalence of abnormal ABPM patterns in overweight or obese children and adolescents was detected. Additionally, OBP poorly correlated with the child’s actual ABPM pattern. Herewith, we emphasized the usefulness of ABPM as an important diagnostic tool in this population.
Socioeconomic status is correlated with adiposity in childhood cancer survivors.
Authors: Štrublová Lucie , Kepák Tomáš , Kuruczová Daniela , Holíková Marta , Bienertová-Vašků Julie , Kepáková Kateřina , Štěrba Jaroslav 
Published: August 2022

Abstract
Purpose
We examined the association between unemployment, cancer type and total body fat percentage in the childhood cancer survivors (CCS) cohort.
Methods
Survivors completed questionnaires on socioeconomic status; body fat percentage was assessed by bio-impedance (normal body fat percentage: 10–20% men; 18–28% women).
Results
The highest unemployment rate was in the group of CCS with a diagnosis of brain and spinal column tumours (72.7%). CCS after brain and spinal column tumours also had a significantly higher percentage of body fat than CCS with other diagnoses (p-value 0.0354). Survivors who did not undergo CNS-directed treatment were 5 times more likely to be employed (p-value 0.013).
Conclusions
Survivors of brain or central nervous system (CNS) cancer in childhood are at major risk of unemployment, which can affect their health and psychosocial integration. At the same time, this group has a significantly higher proportion of body fat than survivors of other diagnoses, which may have a considerable impact on the development of cardiometabolic disorders and their overall quality of life.
Trial registration:
This study was registered on July 29, 2022, at ClinicalTrials.gov (NCT05481229).
Implications for Cancer Survivors:
Interventions should focus on creating support programs that address labour market inclusion hand-in-hand with promoting good lifestyle and body weight reduction.