bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frailty Nurse and GP-led models of care in Care Homes: The role of contextual factors impacting Enhanced Health in Care Homes framework implementation
Authors: (Zeb) Zeibeda Sattar , Young-Murphy Lesley , Craig Lynn , Steven Alison , Wilson-Menzfeld Gemma 
Published: July 2022

Abstract
Background
The Enhanced Health for Care homes (EHCH) framework is an innovative response to provide more proactive, preventative approaches to care for residents living in care homes. It involves co-producing a shared vision with primary care. As part of EHCH a UK clinical commissioning group supported GP's in two localities to implement their preferred delivery approach involving a new Frailty Nurse-led (FN-led) model in care homes alongside an existing General Practitioner-led (GP-led) model. This paper focuses on implementation of the new FN-led model.
Methods
A qualitative study design was adopted. Forty-eight qualitative semi-structured interviews were undertaken across six care home sites in a Northern locality: three implementing the FN-led and three engaged in an existing GP-led model. Participants included residents, family members, care home managers, care staff, and health professionals working within the EHCH framework.
Results
Two overarching themes were generated from data analysis: Unanticipated implementation issues and Unintended outcomes. Unsuccessful attempts to recruit Frailty Nurses (FN) with enhanced clinical skills working at the desired level (UK NHS Band 7) led to an unanticipated evolution in the implementation process of the FN-led model towards ‘training posts’. This prompted misaligned role expectations subsequently provoking unexpected temporary outcomes regarding role-based trust. The existing, well understood nature of the GP-led model may have further exacerbated these unintended outcomes.
Conclusion
Within the broader remit of embedding EHCH frameworks, the implementation of new FN roles needed to evolve due to unforeseen recruitment issues. Wider contextual factors are not in the control of those developing new initiatives and cannot always be foreseen, highlighting how wider factors can force evolution of planned implementation processes with unintended outcomes. However, the unintended outcomes in this study highlight the need for careful consideration of information dissemination (content and timing) to key stakeholders, and the influence of existing ways of working.
Cx43 acts as a mitochondrial calcium regulator that promotes obesity by inducing macrophages polarization
Authors: Zhou Qing , Wang Yuyan , Lu Zongshi , He Chengkang , Li Li , You Mei , Wang Lijuan , Cao Tingbing , Zhao Yu , Li Qiang , Mou Aidi , Shu Wentao , He Hongbo , Zhao Zhigang , Liu Daoyan , Zhu Zhiming , Gao Peng , Yan Zhencheng 
Published: July 2022

Abstract
Background/Objectives:
Metabolic reprogramming initiates pro-inflammatory macrophages polarization that exacerbates adipocyte dysfunction and subsequent obesity. We hypothesize that connexin 43 (Cx43), a widely-expressed gap junction protein, might affect obesity by regulating mitochondrial calcium that participate in macrophage polarization.
Subjects/Methods:
Macrophage specific knockout of Cx43 and their wild-type littermates were fed with high fat diet (HFD) for 16 weeks. The body weight, glucose homeostasis, and inflammation were evaluated. RAW264.7, the murine-derived cell line, were used and treated with palmitic acid (PA) to mimic the obesity environment.
Results:
HFD treatment induced obesity and exacerbated macrophages infiltration with upregulation of macrophages Cx43. Macrophage-specific knockout of Cx43 reduced HFD-induced obesity by alleviating inflammation in adipose tissue, with less M1 pro-inflammatory macrophage polarization. In consistence, inhibition or knockdown of Cx43improved the impaired oxidative phosphorylation (OXPHOS), reduced mitochondria-associated membranes (MAM) and mitochondrial Ca2+ overload in response to PA. Mechanistically, we confirmed an interaction between Cx43 and the mitochondrial Ca2+ uniporter (MCU), and knockdown of Cx43 alleviated PA-induced succinate dehydrogenase (SDH) oxidation by lowering MCU-mediated mitochondrial Ca2+ uptake.
Conclusions:
Cx43 as a mitochondrial Ca2+ regulator that aggravates obesity via promoting macrophages polarized to M1 pro-inflammatory phenotype and suggests that Cx43 might be a promising therapeutic target antagonizing obesity.
“Not just the consequences, but also the pleasurable sex”: a review of the content of comprehensive sexuality education for early adolescents in Rwanda
Authors: Mbarushimana Valens , Goldstein Susan , Conco Daphney Nozizwe 
Published: July 2022

Abstract
Background: Responding to adolescents' educational needs in sexual and reproductive health and rights is central to their sexual health. In 2016, Rwanda introduced the Competence-based curriculum (CBC) that integrated comprehensive sexuality education (CSE). However, globally, the content of CSE is sometimes dissimilar, and little evidence surrounds its scope in many settings. This study aimed to assess major topic areas of the school-based CSE for early adolescents, analyse how this CSE correlates with international guidelines, and make recommendations accordingly.Methods: We reviewed the CBC and conducted semi-structured interviews with key informants concerned with sexuality education to map sexuality education contents and related CSE competences for pre-primary, primary and lower secondary levels. We selected eleven of the 23 CBC documents for the final review. We manually extracted data using a standard form in Microsoft Excel. We conducted a descriptive analysis (frequency tables and charts) of review data and thematically analysed interviews in NVivo 11 for Windows using the International Technical Guidance of Sexuality Education (ITGSE).Findings: Overall, we found 58 CSE competences from the CBC. CSE competences increased with school grades: 4/58 (6.9%) in pre-school, 5/58 (8.6%) in lower-primary, 17/58 (29.3%) in upper-primary, and 32/58 (55.2%) in lower-secondary levels. Subjects of Kinyarwanda (13/58, 22.4%), Biology (10/58, 17.2%), and Social studies (9/58, 15.5%) abounded in CSE. Four ITGSE key concepts abounded most in CSE competences: Sexual and reproductive health (13/58, 22.4%); Human body and development (11/58, 19%); Values, rights, and sexuality (9/58, 15.5%); and Understanding gender (8/58, 13.8%). The CBC CSE covered least the area of Violence and staying safe (2/58, 3.4%). Six of 27 ITGSE topics had only one CSE competence, and eight ITGSE topics were not represented in the CBC. Recommended additional contents are sexual pleasure, sexual orientation, sexual desire and modern contraceptive methods such as condoms.Conclusion: CSE for early adolescents is delivered mainly in three subjects and aligns, to some extent, with the international guidelines for sexuality education. CSE intensifies with school grades and emphasises more on some topics than others that are equally important. The study recommends additional topics for CSE for this age group.
Multimodal 3D mouse brain atlas framework with skull-derived coordinate system
Authors: Perens Johanna , Salinas Casper Gravesen , Roostalu Urmas , Skytte Jacob Lercke , Gundlach Carsten , Hecksher-Sørensen Jacob , Dahl Anders Bjorholm , Dyrby Tim B. 
Published: July 2022

Abstract
Magnetic resonance imaging and light-sheet microscopy enable non-disruptive 3-dimensional imaging of mouse brains. A combination of complementary information from these modalities is essential for studying disease progression, developing effective therapies, and translating findings into clinical procedures. Although both technologies rely on atlas mapping for quantitative analysis, integration of data from these modalities has been challenging due to morphological differences between brain samples. In this study, we have developed a multimodal atlas framework that includes brain templates based on both imaging modalities, region delineations from the Allen’s Common Coordinate Framework, and a skull-derived stereotaxic coordinate system. The coordinate system allows users to precisely translate spatial positions from ex vivo modalities to locations in a living mouse brain. Consequently, the atlas resource, which is accessible via NeuroPedia, will allow the combination of results obtained from in vivo and ex vivo mouse brain measurements and accurate navigation in the brain during stereotaxic interventions.
Fecal microbiota from MRL/Lpr mice exacerbates pristane induced lupus
Authors: Yi Xiaoqing , Huang Cancan , Huang Chuyi , Zhao Ming , Lu Qianjin 
Published: July 2022

Abstract
Background: The roles of gut microbiota in the pathogenesis of SLE have been receiving much attention during recent years. However, it still remains unknown how fecal microbiota transplantation (FMT) and microbial metabolites affect the immune responses and lupus progression. Methods: We transferred the fecal microbiota from MRL/Lpr mice and MRL/Mpj mice or PBS to the pristane-induced lupus mice and observed the disease development of the pristane-induced lupus mice. We also screened the gut microbiota and metabolite spectrums of the pristane-induced lupus mice with FMT via 16S rRNA sequencing, metagenomic sequencing and metabolomics, respectively, followed by the correlation analysis.Results: FMT from MRL/Lpr mice could promote the pathogenesis of the pristane induced lupus mice, which also affected the immune cell profiles in the intestine exclusively the plasma cells. The structures and compositions of microbial communities in gut of the FMT-Lpr mice were different from those of the FMT-Mpj mice and FMT-PBS mice. Some specific microbes such as prevotella taxa were predominantly enriched in the gut microbiome of the FMT-Lpr mice, which were positively associated with the functional pathways such as cyanoamino acid metabolism. Differential metabolites such as valine and L-isoleucine were identified with varied abundance among three groups. The abundance alteration of the prevotella taxa may affect the phenotypic changes such as the proteinuria levels in the pristane-induced lupus mice.Conclusion: These findings further confirmed that gut microbiota play an important role in the pathogenesis of lupus. The intervention of gut microbiome may provide a novel way to treat lupus.
GDReBase: A Comprehensive, Indexed andUpdated Knowledge Base for Relations betweenHuman Gut Microbes and Diseases
Authors: Xu Haolei , Li Xin , Zheng Chenghao , Liu Kaihua , Liu Sitong , Zeng Yufei , Song Ziyang , Cui Shanzhu , Xu Yongdong 
Published: July 2022

Abstract
Background: Gut microbes play a prominent role in many aspects of human health, as seen through the increasing number of related studies. The accumulation of intestinal flora-related studies enables us to better understand the various relationships between human gut microbes and other factors that affect the human body. However, the existing database does not meet the requirements of browsing or retrieving the latest and most comprehensive published data for scientists. Thus, a knowledge base containing the data related to gut microbes and updates occurring in real-time is in great demand.Results: We present a knowledge base of consistently curated relationships between human gut microbes and disease, phenotype, external influence, etc. By continuously and automatically collecting papers published in mainstream journals and using deep learning and NLP methods for entity relationship identification, GDReBase has now integrated data from 86,848 papers, a figure that will continue to grow.Conclusion: GDReBase is a convenient and comprehensive resource for gut microbiology research and can be accessed free of charge at http://122.112.175.241.
TTC39C may be a new target for the treatment of lung adenocarcinoma
Authors: Rong Hao , Jun Peng , Ma Ke , Zhu Jiang , He Jin-Tao 
Published: July 2022

Abstract
Background: The tetratricopeptide repeat domain 39C ( TTC39C ) gene is a novel TTC genes, which mainly mediates the interaction between proteins. It has been shown to be involved in the progression of a variety of tumors. Here we aimed to identify the effect of TTC39C on lung adenocarcinoma, which can be used as potential intervention targets.Methods: Take samples from TCGA database for difference analysis of TTC39C. Transwell experiment was used to detect the ability of cell metastasis. Celigo and MTT assay detected the effect of TTC39C gene subtraction on cell proliferation. FACS was used to detect the effect of TTC39C gene subtraction on apoptosis. Clonal formation experiment was used to detect the effect of TTC39C gene subtraction on clonal ability. Transcriptomics, proteomics and metabolomics were used to study the possible enrichment pathway of TTC39C gene in the progression of lung adenocarcinoma.Results: TTC39C expression was significantly increased in lung adenocarcinoma. the proliferation, metastasis and cloning ability of human lung cancer cells were inhibited, while the apoptosis of cells increased significantly after TTC39C depletion. It is concluded that TTC39C may be involved in the progression of LUAD mainly through metabolic pathway and p53 pathway through transcriptomics combined with proteomics and metabolomics.Conclusion: In summary, TTC39C plays an important role in regulating the proliferation and metastasis of lung adenocarcinoma cells. The main pathways involved in TTC39C in lung adenocarcinoma mainly include energy metabolism and p53 pathway.
Potent and Selective Covalent Inhibition of the Papain-like Protease from SARS-CoV-2
Authors: Sanders Brian , Pokhrel Suman , Labbe Audrey , Mathews Irimpan , Cooper Connor , Davidson Russell , Phillips Gwyndalyn , Weiss Kevin , Zhang Qiu , O'Neill Hugh , Kaur Manat , Ferrins Lori , Schmidt Jurgen , Reichard Walter , Surendranathan Surekha , Parvathareddy Jyothi , Phillips Lexi , Rainville Christopher , Sterner David , Kumaran Desigan , Andi Babak , Babnigg Gyorgy , Moriarty Nigel , Adams Paul , Joachimiak Andrzej , Hurst Brett , Kumar Suresh , Butt Tauseef , Jonsson Colleen , Wakatsuki Soichi , Galanie Stephanie , Head Martha , Parks Jerry 
Published: July 2022

Abstract
Direct-acting antivirals are needed to combat coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The papain-like protease (PLpro) domain of Nsp3 from SARS-CoV-2 is essential for viral replication. In addition, PLpro dysregulates the host immune response by cleaving ubiquitin and interferon-stimulated gene 15 protein (ISG15) from host proteins. As a result, PLpro is a promising target for inhibition by small-molecule therapeutics. Here we have designed a series of covalent inhibitors by introducing a peptidomimetic linker and reactive electrophile onto analogs of the noncovalent PLpro inhibitor GRL0617. The most potent compound inhibited PLpro with kinact/KI = 10,000 M− 1 s− 1, achieved sub-µM EC50 values against three SARS-CoV-2 variants in mammalian cell lines, and did not inhibit a panel of human deubiquitinases at > 30 µM concentrations of inhibitor. An X-ray co-crystal structure of the compound bound to PLpro validated our design strategy and established the molecular basis for covalent inhibition and selectivity against structurally similar human DUBs. These findings present an opportunity for further development of covalent PLpro inhibitors.
Gait stability characteristics in able-bodied individuals during self-paced inclined treadmill walking
Authors: Lu Chenmiao , Al-Juaid Rawan , Al-Amri Mohammad 
Published: July 2022

Abstract
Gait on inclined surfaces requires active neuromuscular control to maintain stability. This study investigated the effects of self-paced incline treadmill walking on gait stability characteristics and the activation of key lower limb muscles. Twenty-seven healthy subjects (mean age: 25.02 ± 2.06 years) walked at their preferred walking speed on an augmented instrumented treadmill for three minutes at three inclination angles (-8°,0°, and 8°). Changes in gait characteristics (i.e., stability, walking speed, spatial-temporal, kinematic, and muscle forces) across inclination angles were assessed using repeated measures ANOVA and Freidman tests. Results showed that inclined treadmill walking had a significant impact on overall gait characteristics, reflecting changes in gait parameters and muscle activations with respect to the inclination of the treadmill. Stability and walking speed had reduced significantly during uphill walking, suggesting that it was the most challenging walking condition. During uphill walking, there was a significant increase in the peak activation of hamstrings, gastrocnemius, vastus intermedius, and vastus lateralis muscles. In contrast, the peak activation of the antagonist muscle groups including the quadriceps, tibialis anterior, and tibialis posterior muscles significantly increased during downhill walking. Our findings demonstrate that able-bodied individuals adopted walking patterns during inclined treadmill walking to maintain a comfortable and safe walking performance. Future studies should consider inclined treadmill walking as a functional assessment tool or as a rehabilitation intervention to improve gait stability by targeting muscle training.
Serum sulfonylurea receptor-1 is correlation to perihematomal edema after intracerebral hemorrhage and as a biomarker of clinical prognosis in patients with intracerebral hemorrhage
Authors: Huang Mingsheng , Ma Ping , Liu Yiheng , Peng Jie , Ma Mincai , Zhang Xiang , Cheng Yuan 
Published: July 2022

Abstract
Background
Sulfonylurea receptor-1(Sur1) and transient receptor potential melastatin 4(Trpm4) are implicated in acute brain injury. We determine whether serum Sur1 and Trpm4 concentrations are associated with spontaneous intracerebral hemorrhage(ICH) related perihematomal edema(PHE) and clinical outcome after ICH.
Methods
Serum Sur1 and Trpm4 concentrations were measured in 51 healthy controls and 51 patients with ICH. modified Rankin Scale (mRS) score and non-contrast computed tomography (NCCT) were recorded to assess perihematomal edema (PHE) severity. Modified Rankin Scale(mRS) score of 4–6 at 3 months after ICH was defined as an unfavorable outcome.
Results
Serum Sur1 and Trpm4 concentrations were substantially higher in patients with ICH than in controls. Peak PHE volume was positively correlated with baseline Sur1(r = 0.302, p = 0.031) and more significant related to peak Sur1(0.346, 0.013). Under receiver operating characteristic curve, peak Sur1 levels remained as an independent predictor of poor outcome (OR1.574, 95% CI, 1.144 to 2.165).
Conclusions
Elevated serum Sur1 levels are related to PHE severity and are independently associated with poor prognosis after ICH, indicating that serum Sur1 may have the potential to be a prognostic biomarker of ICH.
Oligomer-Nanoparticle Release from PLA Bioplastics Catalyzed by Gut Enzymes and Its Acute Inflammatory Effect
Authors: Wang Mengjing , Li Qianqian , Xu Youdong , Yang Junjie , Chua Shae Linn , Jia Linran , Lv Jia , Chen Huaiwen , Huang Changjin , Huang Yichao , Chen Jianmin , Fang Mingliang 
Published: July 2022

Abstract
Although the risks of microplastics in environmental exposure and human health are being increasingly studied, little is known about the behavior of “eco-friendly” bioplastics in humans, especially their effects on our gastrointestinal tract. Here we demonstrate that enzymatic hydrolysis of bio-based polylactic acid (PLA) microplastics rapidly generates an excess of nanoplastic particles by competing for triglyceride-degrading lipase during gastrointestinal processes. These tiny nanoparticles are oligomers formed by hydrophobic-driven self-aggregation, and upon exposure the oligomers and their associated nanoparticles can bioaccumulate in in vitro and several in vivo organs, including the liver, intestine, and even in the brain. Severe intestinal damage and inflammation are also observed, the toxic effect of which is mostly pronounced from hydrolyzed oligomer products. Furthermore, the oligomers’ potential protein target screening using large scale pharmacophore model reveals that oligomers can interact with matrix metallopeptidase 12 protein (MMP12), which is further validated using protein binding assay. A close mechanistic study reveals high binding affinity of oligomers to the catalytic zinc ion finger domain, leading to MMP12 inactivation and mediating the adverse bowel inflammatory effect following PLA oligomer exposure. Since biodegradable plastics are highly proposed as one solution for the global plastic problem, understanding the gastrointestinal fate and toxicity of bioplastics, will provide ground-breaking data on bioplastics as a substantial risk to human health.
Effect of the pulse width of discharging current on deformation behavior of short tube in electromagnetic expansion
Authors: Zhang Xiao , Ouyang Shaowei , Li Xiaoxiang , Du Limeng , Li Liang , Xue Yuan , Deng Fangxiong 
Published: July 2022

Abstract
In order to meet the requirement of manufacturing of tubular parts in variant applications, the study of deformation behavior of tube in electromagnetic expansion has remarkable necessity. Especially, in the electromagnetic expansion of short tube (the tube length shorter than the coil length), the deformation behavior is more complex than the existing studies of electromagnetic expansion of long tubes. In this paper, the effect of different discharging current pulse width on deformation behavior of AA6061 aluminum short tube has been studied and discussed, according to the waveforms of radial Lorentz force, velocity and displacement. Results showed that when the discharging current has short pulse width, the deformed tube has concave profile, while when the discharging current has long pulse width, the displacement of tube middle gradually exceeds that of tube end during the forming process, which means that the deformed tube changes to convex profile.
The association between lumbar cage migration and subsidence on osteogenesis following transforaminal lumbar interbody fusion: A DICOM-based software data analysis
Authors: Rickert Marcus , Wehner Diana , Rahim Tamim , Hölper Bernd , Eichler Michael , Makowski Marcus , Rauschmann Michael , Brenneis Marco 
Published: July 2022

Abstract
Pseudarthrosis following transforaminal interbody fusion (TLIF) is not infrequent. Although cage migration and subsidence are commonly regarded as evidence of the absence of solid fusion, there is still no evidence of the influence of cage migration and subsidence on fusion.
This study aimed to evaluate cage migration and subsidence using computed tomography (CT) DICOM data following lumbar interbody fusion. The effects of cage migration and subsidence on fusion and clinical outcomes were also assessed.
A postoperative CT data set of 67 patients treated with monosegmental TLIF was analyzed in terms of cage position. To assess the effects of cage migration and subsidence on fusion, 12-month postoperative CT scans were used to assess fusion status. Clinical evaluation included the Oswestry Disability Index, and the visual analog scale for pain.
Postoperative cage migration occurred in 85.1% of all patients, and cage subsidence was observed in 58.2%. Radiological signs of pseudarthrosis was observed in 7.5% of the patients Neither cage migration nor subsidence affected the clinical or radiographic outcomes. No correlation was found between clinical and radiographic outcomes.
The incidence of cage migration was considerable. However, as cage migration and subsidence were not associated with bony fusion, their clinical significance was considered limited.