bluuebunny/crossref_metadata_2025_split huggingface dataset:
A van der Waals force-based adhesion study of stem cells exposed to cold atmospheric plasma jets
Authors: Hajizadeh Kobra , Mehdian Hassan , Hajisharifi Kamal , Robert Eric 
Published: March 2022

Abstract
Cold atmospheric plasma has established its effect on cell adhesion. Given the importance of cell adhesion in stem cells, the current study looked at the effect of plasma treatment on stem cell adhesion by which the differentiation and fate of cells are determined. In this paper, adhesion modification was investigated not just among cell-ECM, but also between suspended cells, and enhanced adhesions were found in both circumstances. Regarding the previous works, increasing the cell-ECM adhesion during the plasma therapy could be mostly attributed to enhancing the production and activity of integrin proteins, while at the suspension condition, we have showed that this increasing is due to the enhancing in van der Waals forces (VDWFs) between the cells. Considering the importance of van der Waals forces at the cellular level, the effect of cold plasma on VDWFs and so its effect on adhesion were investigated in this work for the first time, to the best of our knowledge. For this purpose, employing the semi-empirical methods, the role of the plasma therapy on the VDWF between the cells has been studied at three levels; a) plasma-induced dipole formation, b) Hammaker coefficient modification of culture medium, and c) cell roughness modification. We believe that, the present work gives a new physical insight in studying the plasma therapy method at the cellular level.
Changes in rhizosphere microbiomes of field-grown rice in response to biogas slurry application
Authors: Yang Yanmei , He Yan , Sheng Hao , Wei Zheng , Weng Rui , Zhou Gongming , Lin Yuanyuan 
Published: March 2022

Abstract
Biogas slurry (BS) is widely used as a liquid fertilizer in paddy fields. A growing body of evidence shows that rhizosphere microbiota is important for plant growth and reproduction. However, the dynamic responses of rhizosphere microbiomes of field-grown rice to BS application still remain unknown. In this study, a field experiment was conducted to further understand the impacts of BS fertilizer (BCF) on the dynamic changes of rhizosphere microbiota throughout the life cycle of rice with conventional chemical fertilizer (CF) treatment as control. The results demonstrated that BS could increase the N-, P- and C- level in paddy water as well as rhizospheric microbial abundance and diversity of paddy fields compared with control. In particular, the phosphate-solubilizing- and cellulose-decomposition-related bacteria (e.g. Bacillus) and fungi (e.g. Mortierella) were more abundant in the rhizosphere of BCF than those of CF. Moreover, these above-mentioned microbes increased markedly at the reproductive stages compared with those at vegetative stages in BCF, which could promote rice to obtain available nutrient (phosphorus and carbon) at low fertility input stages (the reproductive stages). It was noted that denitrifying-related bacteria (e.g. Steroidobacteraceae) decreased and dissimilatory nitrate reduction to ammonia (DNRA) -related bacteria increased generally at the reproductive stages, which could reduce N-loss and help to N-fixation. Furthermore, correlation network showed that nutrient-cycles-related microbes were more closely interconnected in BCF than control. These observations demonstrated the application of BS could regulate root-related microbiomes towards a beneficial fertilizer use for rice production.
Identification and characterization of the gene profile in diabetes induced erectile dysfunction rat model
Authors: Zhu Zhiguo , Li Xiaoli , Cao Xiande , Qin Huisheng , Yue Dong , Liu Deqian , Tan Guigeng , Xuan Xujun , Zhu Haizhou 
Published: March 2022

Abstract
Background
Genes are differentially expressed in diabetes induced erectile dysfunction (DED); however, there is still a lack of systematic and in-depth understanding of the cellular and molecular mechanisms of DED. This study aimed to systematically analyze molecular correlates leading to ED in diabetes by bioinformatics analysis, and provide new insights into the mechanism of DED.
Results
A total of 800 differentially expressed genes (DEGs, 407 upregulated and 393 downregulated) were identified in the diabetic-induced ED samples compared with the control group. The upregulated DEGs were mainly enriched in glucose and lipid metabolism-related pathways, and the downregulated DEGs were primarily enriched in tissue development and structure. The dysregulated extracellular matrix genes (especially related to collagen and elastin) may be closely related to the damage of erectile function of the corpus cavernosum. Sixteen hub genes (6 upregulated, 10 downregulated) and 24 modules were detected buy hub gene and MCODE analysis. Protein phosphorylation might play an important role in DED. Furthermore, 20 TFs targeting DEGs were identified by ChEA3.
Conclusions
Our research comprehensively and systematically described the molecular characteristics of DED, and suggested that dysregulated extracellular matrix genes and protein phosphorylation may play critical roles in DED.
Extraventricular Neurocytoma: a Case Report and Literature Review
Authors: Wang Liping , Dai Xiaoxiao , Ping Yifang , Chen Ping , Chen Yanming , Cheng Shan , Li Yongdong , Li Suwen , Li Haoran , Yuan Jiaqi , Hou Yanghao , Dong Jun 
Published: March 2022

Abstract
Extraventricular neurocytoma (EVN) is a rare intracranial tumor. The most common locations of EVN were frontal lobe and cerebellum, followed by temporal lobe. Heterogeneous contrast enhancement, cystic changes, perilesional edema and calcification were a few distinct imaging characteristics. EVN is a primary tumor with either glial or neuronal differentiation or potential for atypical changes. The lack of characteristic manifestations in histopathology makes EVN difficult to distinguish from other low-grade neuronal and mixed neuronal-glial tumors. Herein, we describe a case of frontal extraventricular neurocytoma in a 37-year-old male presenting with dizzy and review the literature on this rare tumor. Pathological examination reveals that the tumor mainly composed of oligodendroglia-like cells, with expression of synaptophysin (SYN) and Olig2 (oligodendrocyte transcription factor 2), and microcalcifications. Methylation profile and t-SNE analysis show our sample cluster with the other two reference EVNs. Next-generation sequencing (NGS) shows FGFR1-TACC1 fusion. We report a case of EVN with expression of Olig2, FGFR1-TACC1 fusion, methylation of MGMT promoter, and discuss our experience along with a review of the published literature.
Meta-Analysis Of Ecological Studies Attenuating Solar Radiation Illustrates The Importance Of Blue Light Over Ultraviolet Radiation In Driving Photodegradation Of Litter In Terrestrial Ecosystems
Authors: Wang Qing-Wei , Pieristè Marta , Kotilainen Titta K. , Forey Estelle , Chauvat Matthieu , Kurokawa Hiroko , Robson T Matthew , Jones Alan G 
Published: March 2022

Abstract
Background and aim: Wherever sunlight reaches litter there is potential for photodegradation to contribute to decomposition. Although recent studies have weighted the contribute of the short-wavelength visible spectrum and ultraviolet (UV) radiation as drivers of photodegradation, the relative importance of each spectral region across biomes and plant communities remains uncertain.Methods: We performed a systematic meta-analysis of studies that assessed photodegradation through spectrally selective attenuation of solar radiation, by synthesizing 30 published studies using field incubations of leaf litter from 110 plant species under ambient sunlight.Results: Globally, the full spectrum of sunlight significantly increased litter mass loss by 15.3% ± 1% across all studies compared to darkness. Blue light alone was responsible for most of this increase in mass loss (13.8% ± 1%), whereas neither UV radiation nor its individual constituents UV-B and UV-A radiation had significant effects at the global scale, being only important in specific environments. These waveband-dependent effects were modulated by climate and ecosystem type. Among initial litter traits, carbon content, lignin content, lignin to nitrogen ratio and SLA positively correlated with the rate of photodegradation. Global coverage of biomes and spectral regions was uneven across the meta-analysis potentially biasing the results, but also indicating where research in lacking.Conclusions: Across studies attenuating spectral regions of sunlight, our meta-analysis confirms that photodegradation is a significant driver of decomposition, but this effect is highly dependent on the spectral region considered. Blue light was the predominant driver of photodegradation across biomes rather than UV radiation.
Percutaneous vertebral-disc plasty for thoracolumbar very severe osteoporotic vertebral compression fractures
Authors: Jiang Jiawei , Zhang Jinlong , Bao Guofeng , Chen Jiajia , Wu Chunshuai , Xue Pengfei , Xu Guanhua , Cui Zhiming 
Published: March 2022

Abstract
Background: To compare the clinical outcomes and radiological parameters of patients undergoing percutaneous vertebroplasty (PVP) versus those undergoing percutaneous vertebral-disc plasty (PVDP) for back pain, segmental instability, and kyphosis due to thoracolumbar very severe osteoporotic vertebral compression fractures (vsOVCFs).Methods: 31 patients with thoracolumbar vsOVCFs were retrospectively reviewed. All the patients were divided into the PVP group and the PVDP group. The visual analogue scale (VAS), Oswestry Disability Index (ODI), local kyphosis angle, and disc height were recorded preoperatively and postoperatively.Results: Significant postoperative improvements on the VAS, ODI, and the local kyphosis angle (LKA) were shown, compared with the preoperative values in both groups (p < 0.05). The average VAS, ODI, and LKA for patients in the PVP group were increased compared to those in the PVDP group were observed at the last follow-up (p < 0.05). The DHA, DHP, and LKA were seen to be maintained in the PVDP group at the last follow-up (p > 0.05). In those parameters, the change was significantly lower in the PVDP group at the last follow-up. (p <0.05).Conclusions: PVDP may be a feasible and effective technique for the treatment of very severe OVCFs, that can restore intervertebral height, provide segmental stabilizing and improve kyphosis in the short term.
Identification of a pyroptosis-related prognostic signature in breast cancer
Authors: Chen Hanghang , Luo Haihua , Wang Jieyan , Li Jinming , Jiang Yong 
Published: March 2022

Abstract
Background
The relationship between pyroptosis and cancer is complex. It is controversial that whether pyroptosis represses or promotes tumor development. This study aimed to explore prognostic molecular characteristics to predict the prognosis of breast cancer (BRCA) based on a comprehensive analysis of pyroptosis-related gene expression data.
Methods
RNA-sequcing data of BRCA were collected from The Cancer Genome Atlas (TCGA) and Gene Expression Ominibus (GEO) datasets. First, pyroptosis-related differentially expressed genes (DEGs) between normal and tumor tissues were identified from the TCGA database. Based on the DEGs, 1053 BRCA patients were divided into two clusters. Second, DEGs between the two clusters were used to construct a signature by a least absolute shrinkage and selection operator (LASSO) Cox regression model, and the GSE20685 cohort was used to validate the signature. Various statistical methods were applied to assess this gene signature. Finally, Single-sample gene set enrichment analysis (ssGSEA) was employed to compare the enrichment scores of 16 types of immune cells and 13 immune-related pathways between the low- and high-risk groups. We calculated the tumor mutational burden (TMB) of TCGA cohort and evaluated the correlations between the TMB and riskscores of the TCGA cohort. We also compared the TMB between the low- and high-risk groups.
Results
A total of 39 pyroptosis-related DEGs were identified from the TCGA-breast cancer dataset. A prognostic signature comprising 21 genes in the two clusters of DEGs was developed to divide patients into high-risk and low-risk groups, and its prognostic performance was excellent in two independent patient cohorts. The high-risk group generally had lower levels of immune cell infiltration and lower activity of immune pathway activity than did the low-risk group, and different risk groups revealed different proportions of immune subtypes. The TMB is higher in high-risk group compared with low-risk group. OS of low-TMB group is better than that of high-TMB group
Conclusion
A 21-gene signature comprising pyroptosis-related genes was constructed to assess the prognosis of breast cancer patients and its prognostic performance was excellent in two independent patient cohorts. The signature was found closely associated with the tumor immune microenvironment and the potential correlation could provide some clues for further studies. The signature was also correlated with TMB and the mechanisms are still warranted.
Abnormal Proliferation of Gut Mycobiota Contribute to The Aggravation of Type 2 Diabetes
Authors: Bao Li , Zhang Ying , Zhang Guo , Jiang De , Yan Dan 
Published: March 2022

Abstract
Type 2 diabetes (T2D) constitute a worldwide health threat, and the underlying mechanism for the development and progression of T2D is complex and multifactorial. During the last decade, gut commensal bacteria have been found to play a crucial role in the regulation of T2D and related metabolic disorders. However, as a considerable component in gut microbiome, the relationship between mycobiota and T2D and related metabolic disorders remains unclear. As a proof-of-concept, we observed that the ablation of the commensal fungi in mice can protect HFD induced insulin resistance and related metabolic disorders. Both ITS2 sequencing and culture-dependent analysis show the enrichment of Candida albicans in samples from individuals with T2D (Chinese Clinical Trial Registry, ChiCTR2100042049). Repopulation with C. albicans in HFD mice accelerated insulin resistance and related disorders. Mechanically, we found the β-glucan from C. albicans mirrored the deteriorating effect of C. albicans through the dectin-1 dependent pathway. Our current findings support that gut mycobiota play an important role in the progress of T2D and indicated the preventing of gut mycobiota is a promising strategy to alleviate insulin resistance and related metabolic dysfunctions.
Prognosis of resected non-small cell lung cancer with pleural plaques on intrathoracic findings
Authors: Mimura Takeshi , Kagimoto Atsushi , Kamigaichi Atsushi , Yamashita Yoshinori 
Published: March 2022

Abstract
Background: The prognosis of patients with lung cancer who demonstrate pleural plaques intraoperatively, which may be associated with exposure to asbestos, is unclear. Here, we compared the clinicopathological characteristics and prognosis of these patients with those of patients without pleural plaques.Methods: We included patients who underwent curative-intent resection for non-small cell lung cancer. We retrospectively investigated the relationship of intrathoracic findings of pleural plaques with clinicopathological features and prognosis.Results: Pleural plaques were found in 121/701 patients (17.3%) during surgery. The incidence of squamous cell carcinoma (P < 0.001) and the pathological stage (P = 0.021) were higher in patients with pleural plaques. Overall survival was significantly worse in patients with pleural plaques (5-year rate; 64.5% vs. 79.3%; P < 0.001), and the same finding was noted in clinical stage I patients (5-year rate; 64.8% vs. 83.4%; P < 0.001). In multivariable analysis, the presence of pleural plaques was a significant predictor of overall survival in patients with clinical stage I (hazard ratio, 1.631; P = 0.039). Among patients with emphysema severe than Goddard score 5 points or interstitial pneumonia, overall survival was also significantly worse in patients with pleural plaques (5-year rate; 66.3% vs. 49.5%; P < 0.001).Conclusions: Patients with non-small cell lung cancer who underwent resection and demonstrated pleural plaques intraoperatively had a significantly worse prognosis. It is important to recognize the presence of pleural plaques intraoperatively, and our findings will be useful in determining the treatment and follow-up strategy for such patients with lung cancer and pleural plaques on intrathoracic examination.
Degradation of polycyclic aromatic hydrocarbons during decontamination of oil-polluted soils: the power of electronic bioinformatics databases
Authors: Ablieieva Iryna , Plyatsuk Leonid , Liu Tong , Berezhna Iryna , Yanchenko Ilona 
Published: March 2022

Abstract
The main idea was to solve the problem related to oil contamination of soil using bioremediation with bioaugmentation with modelled microorganism strains. The paper aimed to develop a bacterial consortium for petroleum hydrocarbons degradation during biological treatment of oil-contaminated soils using electronic databases. The methodology of the research included analysis of the mechanisms and metabolic pathways of petroleum hydrocarbons degradation, and assessment of the possible reaction modulus and enzymatic systems for degradation of aromatic compounds. The taxonomic classification and review of metabolic pathways of oil compound transformation were carried out using electronic KEGG, MetaCyc and EzTaxon databases. The KEGG database was used to create a microbiological consortium of certain strains of bacteria that improved hydrocarbon degradation process performance. Identification of bacteria complete genome using Island Viewer 4 allowed to create of a consortium of oil-destructive bacteria consisting of such strains: Pseudoxanthomonas spadix BD-a59, Rhodococcus jostii RHA1, Rhodococcus aetherivorans IcdP1, Pseudomonas putida ND6, Pseudomonas stutzeri 19SMN4, Pseudomonas fluorescens UK4, Acinetobacter lactucae OTEC-02, Bacillus cereus F837/76.7.9. The ratio between the mentioned strains of microorganisms in the consortium was set at 20% : 20% : 15% : 10% : 10% : 5% : 5% : 15%. This bacterial consortium for aromatic hydrocarbons was created according to the metabolic information of basic enzymatic systems and predominant transformation of particular oil compounds, using BacDive database.
Factors Associated With Children’s HIV Positive Status Disclosure in Wolaita Zone, Southern Ethiopia: Cross-sectional Study
Authors: Walle Belete Gelaw , Tiruneh Chalie Marew , Dessie Tigabu , Selomon Nigusie , Kassaw Amare , Chekole Bogale , Wubneh Moges , Lankrew Tadele , Bayih Wubet Alebachew 
Published: March 2022

Abstract
Background: Children HIV-positive status disclosure is an essential component of chronic care and long-term disease management. However, children HIV-positive status disclosure is complex and varies across different communities. Although data from different settings are necessary to overcome this problem, evidences are limited particularly in case of children HIV-positive status disclosure. Therefore, this study aims to assess the prevalence of HIV-positive status disclosure and associated factors among children on antiretroviral therapy.Methods: A facility based, mixed-method study was conducted among 203 care givers with children in Wolaita Zone, Southern Ethiopia. Structured questionnaire and in-depth interview guide was implemented to collect quantitative and qualitative data, respectively. Simple random sampling for quantitative and purposive sampling for qualitative parts was applied. Content or Thematic analysis for qualitative and Binary logistic regression was used for quantitative. Results: In this study, the overall prevalence of children HIV-positive status disclosure was found to be 46.8%. Age of the child >=12 years (AOR= 7.5, 95% CI: 2.9-15.6), duration on ART >=72 months (AOR= 3.8, 95% CI: 1.7-6.7), death of parent (AOR= 2.0, 95% CI: 1.1- 3.8), and types of health facilities (AOR= 2.1, 95% CI: 1.3-4.7) were associated with disclosure. Being immature child was the commonest reason of non-disclosure. Frequent questions by the child why they are taking drugs, for better self-care, and treatment adherence were the commonest reason of care givers to make decision for disclosing their children’s HIV positive status.Conclusion: Children’s HIV-positive status disclosure was low in this study. Health facility, children, and care givers related factors were mentioned as reason for disclosing or not-disclosing children’s HIV-positive status. Hence, health care workers should give especial attention for children HIV-positive status disclosure which helps to increase the effectiveness of treatment out-come and prevent further HIV transmission.
Large-area flexible Visible-Short wavelength Infrared Photodetection based on III-V compound semiconductor membrane photodetector with high performance
Authors: LI XUANZHANG , ZHANG JUNYANG , YUE CHEN , TANG XIANSHENG , GAO ZHENDONG , JIANG YANG , DU CHUNHUA , DENG ZHEN , JIA HAIQIANG , WANG WENXIN , CHEN HONG 
Published: March 2022

Abstract
Mechanically flexible optoelectronic devices and systems can enable a much broader range of applications than what their rigid counterparts can do, especially for novel bio-integrated optoelectronic systems, flexible consumer electronics and wearable sensors. Inorganic semiconductor could be a good candidate for the flexible PD when it can keep its high performance under the bending condition. Here, we demonstrate a III-V material-based flexible photodetector operating wavelength from 640 nm to 1700 nm with the high detectivity of 5.18×1011 cm‧Hz1/2/W @1550nm by using a simply top-to-down fabrication process. The optoelectrical performances are stable as the PDs are exposed to bending cycles with a radius of 15 mm up to 1000 times. Furthermore, the mechanical failure mode of the PD is also investigated, which suggests that the cracking and delamination failure mode are dominant in bending up and bending down direction, respectively. Such a flexible III-V material-based PD and design with stable and high performance could be a promising strategy for the application of the flexible broad spectrum detection.
Source apportionment of metal elements in a river by using an effective variance chemical mass balance model: a feasibility study
Authors: HSIEH PEIYUAN , Ho Chi-Chang , Wang Gen-Shuh , Hsu Yuan-Jeng , Wang Di-Wen , Shang Yu-Man , Wu Chang-Fu 
Published: March 2022

Abstract
This technical report aimed to determine the sources of water pollution in a major river in northern Taiwan by using the characteristics of actual industrial wastewater and the effective variance chemical mass balance (EV-CMB) model. River water samples from 9 sampling sites and 14 profiles of potential sources were collected and a total of 52 metal elements were analyzed. To monitor the levels of industrial pollutants in the water samples, the metal index (MI) is used. The model results identified the major sources of river pollution and may suggest the existence of unknown sources. This study demonstrates the feasibility of applying the receptor model in the strategic investigation of river pollution and highlights the need for a comprehensive source profile database.