bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical efficacy of hyperbaric oxygen combined with different timings of right median‐nerve electrical stimulation in patients with brain injury‐induced disorders of consciousness
Authors: Liu Yan‐Song , Liu Zi‐bo , Yang Zhe , Zhao Long , Li Hong‐Ling 
Published: August 2022

AbstractObjectiveThe objective of this study was to investigate the clinical efficacy of hyperbaric oxygen combined with right median‐nerve stimulation (RMNES) in patients with disorders of consciousness caused by brain injury.MethodsA total of 120 patients with consciousness disorders caused by brain injury were selected. They were randomly divided into three groups, a control group, test group 1 (treated with RMNES after hyperbaric oxygen therapy [HBOT]), and test group 2 (treated with RMNES at the same time as HBOT), with 40 patients in each group. Before and after treatment, the Glasgow coma scale (GCS), brainstem auditory‐evoked potential (BAEP), electroencephalogram (EEG), and upper‐limb sensory‐evoked potential (USEP) were evaluated for the three groups of patients.ResultsThe GCS score of patients in the three groups significantly improved compared with that before treatment (p < .05). There were significant differences in GCS scores among the three groups (p < .05), and the GCS score for the patients was test group 2>test group 1>control group. The EEG, BAEP, and USEP scores were significantly improved compared with those before treatment (p < .05), and the degree of improvement of patients in the three groups was test group 2>test group 1>control group (p < .05). The clinical efficacy of test group 2 was higher than that of test group 1, and the clinical efficacy of test group 1 was higher than that of the control group (p < .05).ConclusionHyperbaric oxygen combined with RMNES can improve the state of consciousness and promote the recovery of consciousness for patients with consciousness disorders caused by brain injury, and the effect of RMNES combined with HBOT in the chamber on improving the recovery of consciousness is better than after HBOT outside the chamber.
Expressing antigen binding fragments with high titers in a targeted integration CHO host by optimizing expression vector gene copy numbers and position: A case study
Authors: Tang Danming , Gunson Jane , Tran Eric , Lam Cynthia , Shen Amy , Snedecor Brad , Barnard Gavin , Misaghi Shahram 
Published: August 2022

AbstractAntigen binding fragments (Fab) are a promising class of therapeutics as they maintain high potency while having significantly smaller size relative to full‐length antibodies. Because Fab molecules are aglycosylated, many expression platforms, including prokaryotic, yeast, and mammalian cells, have been developed for their expression, with Escherichia coli being the most commonly used Fab expression system. In this study, we have examined production of a difficult to express Fab molecule in a targeted integration (TI) Chinese Hamster Ovary (CHO) host. Without a need for extensive host or process optimization, as is usually required for E. coli, by simply using different vector configurations, clones with very high Fab expression titers were obtained. In this case, by increasing heavy chain (HC) gene copy numbers, clones with titers of up to 7.4 g/L in the standard fed‐batch production culture were obtained. Our findings suggest that having a predetermined transgene integration site, as well as the option to optimize gene copy number/dosage, makes CHO TI hosts an effective system for expression of Fab molecules, allowing Fab expression using platform process and without significant process development efforts.
Immune checkpoint inhibitors as first line in advanced melanoma: Evaluating progression‐free survival based on reconstructed individual patient data
Authors: Ossato Andrea , Damuzzo Vera , Baldo Paolo , Mengato Daniele , Chiumente Marco , Messori Andrea 
Published: August 2022

AbstractBackgroundIn patients with advanced melanoma, immune‐checkpoint inhibitors (ICIs) represent the mainstay for first line treatment. Recently, relatlimab+nivolumab was proposed as a new combination therapy. This review was aimed at summarizing the current data of effectiveness for ICIs. Progression‐free survival (PFS) was the endpoint of our analysis.MethodsAfter a standard literature search, Phase II/III studies comparing different ICI regimens in previously untreated advanced melanoma patients were analyzed. Patient‐level data were reconstructed from Kaplan–Meier curves by application of the IPDfromKM method. These reconstructed datasets were used to perform indirect comparisons between treatments. Standard statistical testing was used, including hazard ratio and medians. A secondary analysis employed the restricted mean survival time.ResultsSix trials were included in our analysis. Information on PFS from these trials was pooled according to the following treatments: nivolumab or pembrolizumab as monotherapy, or in combination with ipilimumab, and relatlimab + nivolumab. Pembrolizumab+ipilimumab showed significantly better PFS compared with the other treatments; nivolumab+ipilimumab ranked second; the other treatments showed a similar survival pattern.ConclusionsThe picture of comparative effectiveness resulting from our analysis is complex. The IPDfromKM method is advantageous because it accounts for the length of follow‐up but loses the balance between treatment group and controls determined by randomization. Based on indirect comparisons, the combination of pembrolizumab+ipilimumab showed a particularly high efficacy, and so deserves further investigation. While the effect of between‐trial differences in inclusion criteria plays an important role, our results do not support the proposal of relatlimab+nivolumab as a new standard of care.
Alexithymia Reduction Treatment: A pilot quasi‐experimental study for remediation of alexithymia and its consequent effects on the general mental health of university students
Authors: Akram Ayesha , Arshad Tehreem 
Published: August 2022

AbstractObjectivesAlexithymia is the inability of an individual to adequately recognise and describe their emotions, which directly affects mental health by precipitating psychological disorders or by causing a disturbance to interpersonal functioning, coping responses, and quality of life. The purpose of this research was to test the efficacy of alexithymia reduction treatment (ART), a pilot online group intervention specifically designed to ameliorate alexithymia in men.MethodsAs this was a pilot study, a small sample consisting entirely of male university students (n = 20) was selected. The participants were screened on the standardised measures at pre‐treatment and post‐treatment and were assigned to a treatment group (n = 10) or wait‐list control group (n = 10). The study utilised a quasi‐experimental design as the participants were assigned to the treatment and wait‐list control group based on their availability to attend the therapeutic sessions. The treatment group then received ART while the wait‐list control received no treatment at that time.ResultsThe statistical analysis showed a significant reduction in the participants' alexithymia, as well as depression and anxiety, in the treatment group but not in the wait‐list control group.ConclusionART proves to be efficacious in reducing alexithymia. In addition, the treatment modality proved to be useful in improving the participants' general psychological distress (anxiety, depressive symptoms).
Microwave‐Assisted Enzymatic Extraction, Partial Characterization, and Antioxidant Potential of Polysaccharides from Sagittaria trifolia Tuber
Authors: Zhang Yang , Wang Jiayi , Yang Jingchun , Li Yingjie , Zhang Wen , Liu Shuyue , Yang Guihong , Yan Zhaowei , Liu Yang 
Published: August 2022

AbstractSagittaria trifolia tuber is an aquatic vegetable. In this work, microwave‐assisted enzymatic extraction (MEE) was used to extract S. trifolia tuber polysaccharides (STTPs). Optimum conditions were complex enzyme of 2 %, liquid‐to‐solid ratio of 43 : 1 mL g−1, microwave power of 506 W, and time of 8 min, under which STTPs yield was 36.22±0.69 %, higher than those of other methods. STTPs were sulfated polysaccharides with sulfur valence of S6+. STTPs comprised mannose, glucose, galactose, and arabinose at a mole ratio of 3.69 : 19.33 : 6.21 : 1.00, molecular weights of 3606 kDa and 149.6 kDa, particle size of 220 nm, and zeta potential of −5.02 mV. The surface of STTPs was full of bumps and holes, and abundant in O1s and non‐functionalized C1s. STTPs would scavenge reactive oxygen species with advantage. It would provide an efficient MEE method to obtain antioxidant STTPs, also a clue for extracting polysaccharides from starch‐rich crops.
Microwave‐Assisted Three‐Component Synthesis of Novel N‐Arylated‐Dihydrobenzo[g]quinoline‐5,10‐Diones and Their Potential Cytotoxic Activity
Authors: Nguyen Ha Thanh , Le‐Nhat‐Thuy Giang , Thi Phuong Hoang , Thi Quynh Giang Nguyen , Nguyen Tuan Anh , Thi Thu Ha Nguyen , Thi Tuyet Anh Dang , Nguyen Tuyen Van 
Published: August 2022

AbstractA convenient three‐component synthetic approach was developed en route to new and significative N‐arylated‐dihydrobenzo[g]quinoline‐5,10‐diones using 2‐hydroxy‐1,4‐naphthoquinone, a variety of aromatic aldehydes, and 4‐(arylamino)furan‐2(5H)‐ones. A sequence of steps including Knoevenagel condensation, Michael addition, [1,3]‐hydrogen shift, intramolecular cyclization and dehydration led to the formation of products. All the products were structurally characterized by spectroscopic techniques and assessed in terms of their cytotoxicity profile against four cancer cell lines (KB, HepG2, A549, and MCF7), and human embryonic kidney (Hek‐293) cell lines.
Total Chemical Synthesis of a Functionalized GFP Nanobody
Authors: Huppelschoten Yara , Elhebieshy Angela F. , Hameed Dharjath S. , Sapmaz Aysegul , Buchardt Jens , Nielsen Thomas E. , Ovaa Huib , van der Heden van Noort Gerbrand J. 
Published: August 2022

AbstractChemical protein synthesis has proven to be a powerful tool to access homogenously modified proteins. The chemical synthesis of nanobodies (Nb) would create possibilities to design tailored Nbs with a range of chemical modifications such as tags, linkers, reporter groups, and subsequently, Nb‐drug conjugates. Herein, we describe the total chemical synthesis of a 123 amino‐acid Nb against GFP. A native chemical ligation‐ desulfurization strategy was successfully applied for the synthesis of this GFP Nb, modified with a propargyl (PA) moiety for on‐demand functionalization. Biophysical characterization indicated that the synthetic GFP Nb‐PA was correctly folded after internal disulfide bond formation. The synthetic Nb‐PA was functionalized with a biotin or a sulfo‐cyanine5 dye by copper(I)‐catalyzed azide‐alkyne cycloaddition (CuAAC), resulting in two distinct probes used for functional in vitro validation in pull‐down and confocal microscopy settings.
Tetrazine‐Triggered Bioorthogonal Cleavage of trans‐Cyclooctene‐Caged Phenols Using a Minimal Self‐Immolative Linker Strategy**
Authors: Keppel Patrick , Sohr Barbara , Kuba Walter , Goldeck Marion , Skrinjar Philipp , Carlson Jonathan C. T. , Mikula Hannes 
Published: August 2022

AbstractBond‐cleavage reactions triggered by bioorthogonal tetrazine ligation have emerged as strategies to chemically control the function of (bio)molecules and achieve activation of prodrugs in living systems. While most of these approaches make use of caged amines, current methods for the release of phenols are limited by unfavorable reaction kinetics or insufficient stability of the Tz‐responsive reactants. To address this issue, we have implemented a self‐immolative linker that enables the connection of cleavable trans‐cyclooctenes (TCO) and phenols via carbamate linkages. Based on detailed investigation of the reaction mechanism with several Tz, revealing up to 96 % elimination after 2 hours, we have developed a TCO‐caged prodrug with 750‐fold reduced cytotoxicity compared to the parent drug and achieved in situ activation upon Tz/TCO click‐to‐release.
Leser‐Trélat syndrome secondary to non‐small‐cell lung carcinoma
Authors: Khemakhem Rim , Kallel Nesrine , Jarraya Rahma , Yangui Ilhem , Kammoun Samy 
Published: August 2022

AbstractThe syndrome of Leser‐Trélat (LT) is a rare paraneoplastic syndrome. However, patients presenting with the sign of Leser‐Trélat should be considered to harbor an occult malignancy or a progressive tumor disease until “proven” otherwise. Herein, we present two cases of non‐small‐cell lung carcinoma associated with LT syndrome.
An incidental finding of unroofed coronary sinus during angiography confirmed by 3D echocardiography; A case report
Authors: Fazlinezhad Afsoon , Keihanian Faeze , Ahmadi Mostafa , Tayyebi Mohammad 
Published: August 2022

AbstractUnroofed coronary sinus (UCS) is an uncommon congenital heart anomaly and the most unusual type of atrial septal defect. This report presents a 71‐year‐old female with palpitation and dyspnea following an emotional stress, who was referred for ablation of typical atrial flutter. Finally diagnosed with a partially unroofed CS causing a bidirectional shunt.
The use of a simultaneous fascia lata‐free flap with cochlear implantation after radical Mastoidectomy
Authors: May Jason G. , Kerr John , May Jack A. , Tassone Patrick , Rivera Arnaldo L. 
Published: August 2022

AbstractPatients undergoing cochlear implant after prior radical mastoidectomy are at increased risk of device infection requiring device explant. Various techniques including two‐stage operations have been used. We report the novel technique with use of a vascularized fascia lata free flap for a patient undergoing cochlear implantation with radical mastoidectomy.
COVID‐19‐associated mucormycosis involving the maxilla
Authors: Hasheminasab Mahboube , Karizmeh Mojtaba Salehi , Sharifi Reza , Beshkar Majid , Matloubi Narges , Asadi Amir Ali , Nazar Elham 
Published: August 2022

AbstractIt is important to increase the awareness and knowledge of head and neck surgeons about the recent surge of craniofacial mucormycosis in COVID‐19 patients because early diagnosis and appropriate treatment are essential to improve the outcomes. Here, we describe clinical features, treatment protocols, and outcomes of treatment in eight patients with COVID‐19‐associated mucormycosis in the maxilla. Consistent with the findings of previous studies, our experience in the management of these eight patients shows that early administration of amphotericin B and prompt aggressive surgery are essential for optimal control of the disease.
Sarcoidosis with multiple organ involvement and uncommon symptoms: A case report
Authors: Khalaji Amirmohammad , Rezaei Somaye Sadat , Shajari Rasoul , Masoumi Maryam 
Published: August 2022

AbstractSarcoidosis is a complicated inflammatory disease characterized by the formation of non‐caseating epithelioid granulomas in many organs. Herein, we reported a sarcoidosis case with multiple organ involvements and our diagnostic criteria and treatment plan.
Mucus plugs in peripheral bronchi: An unusual initial manifestation of allergic bronchopulmonary aspergillosis
Authors: Hiratani Nao , Okazaki Akihito 
Published: August 2022

AbstractAlthough allergic bronchopulmonary aspergillosis can be associated with mucus plugs in the central bronchi, this association in the peripheral bronchi remains unclear. A 78‐year‐old woman presented with mucus plugs in both the peripheral and the central bronchi in the right lung, which evolved into consolidation with high‐attenuation mucus after one month.
Anaphylaxis to Moringa oleifera in North Africa: A case report and review of the literature
Authors: Ichrak Bannour 
Published: August 2022

AbstractWe describe here the third reported case of anaphylaxis after ingestion of some leavers of Moringa oleifera, causing a widespread angioedema, a respiratory distress, and an elevation of serum tryptase. M. oleifera leaves were confirmed as the causative allergen by prick testing with fresh leavers.
Simultaneous occurrence of subarachnoid hemorrhage and cerebral venous sinus thrombosis: A systematic review of cases
Authors: Jesuyajolu Damilola , Olukoya Olatomiwa , Moti Terngu 
Published: August 2022

AbstractAlthough the leading causes of subarachnoid hemorrhage (SAH) are aneurysm rupture and arteriovenous malformations, cerebral venous sinus thrombosis (CVST) can, in rare cases, be associated with SAH. This phenomenon is an uncommon presentation, with less than a hundred cases reported based on our review of the literature. The purpose of this review is to highlight what is known regarding these cases, how they are managed and to highlight the need for further studies that will serve as a basis for the development of a standard management guideline across board. The following databases were searched: PubMed and Ovid Embase. A complementary search of Google Scholar and AJOL was done. Gray literature search was also conducted on the Google search engine for any additional relevant papers. We were able to extract data regarding 33 cases from 29 identified studies. The mean age was 46.6 ± 14.08. 17 (51.5%) of the cases were female, and the female‐to‐male ratio is 1.1:1. Headache was by far the commonest symptom, occurring in 82% of cases followed by seizures in 42% of cases. Four patients (12%) had loss of consciousness while 5 patients (15%) had some form of focal neurologic deficit. Twenty patients had cerebral venous sinus thrombosis in at least two different sinuses. The superior sagittal sinus was the most common location for CVSTs (79%), followed by the transverse sinus (57.5%). Twenty‐nine cases (89%) were managed with anticoagulation alone and one case had a mechanical thrombectomy. We have performed a comprehensive review of cases that had the simultaneous occurrence of SAH and CVST and have identified their peculiarities and the challenges to management. Further research is needed in order to identify a causal relationship and to serve as a basis for the development of a standard management guideline across the board.
Nano‐Au Anchored in Organic Base Group‐Grafted Silica Aerogel: A Durable and Robust Catalysts for Green Oxidative Esterification of Furfural
Authors: Song Fan , Cen Shuangshuang , Wan Cong , Wang Lijun 
Published: August 2022

AbstractInorganic metal (hydro)oxide supports with appropriate basicity or acid‐base properties can promote oxidative esterification of furfural over Au‐based catalysts, however, they suffer the problems of easy inactivation induced by the leaching of the components in this water‐producing reaction and the shield of catalytic sites by product adsorption. In this work, we report efficient and stable oxidative esterification of furfural over Au nanoparticles anchored in aminopropyl‐grafted silica aerogel. The support is insoluble and possesses no strong Lewis acid sites to adsorb oxygen‐containing products or intermediates, reducing the probability of catalyst deactivation induced by adsorption. In 7 cycles of reactions, the catalyst maintained the initial activity without any treatments, the yields of alkyl furoate kept above 98 %, and no agglomeration and leaching of Au occurred. Moreover, the roles of Au, alkyl chain, −NH2 group and Au‐activated O species in reactions were clarified, and accordingly, a possible catalytic mechanism involving oxidative dehydrogenation was given.
Selective Synthesis of Primary Amines by Kinetic‐based Optimization of the Ruthenium‐Xantphos Catalysed Amination of Alcohols with Ammonia
Authors: Heider Christian , Pietschmann Dominik , Vogt Dieter , Seidensticker Thomas 
Published: August 2022

AbstractThe selective synthesis of primary amines directly from several alcohols and ammonia using a homogeneous catalyst based on HRuCl(CO)(PPh3)3 and Xantphos is presented. The key to success was the detailed understanding of all mutually influencing parameters such as temperature, ammonia excess, and substrate concentration. These studies were supported by the determination of the kinetics, which allowed the reaction order to be calculated as 0.7. Furthermore, the kinetic model derived from the mechanism was confirmed. After measuring reaction profiles for all influencing parameters, optimized conditions were obtained, which finally allowed the amination of aliphatic, cyclic, as well as primary and secondary alcohols with selectivities to the desired primary amine exceeding 90 % at quantitative alcohol conversion with only minimal formation of the undesired secondary amines. Furthermore, the catalytic activity of the commercially available and robust Xantphos system was drastically improved, corresponding to a turnover frequency (TOF)>60 h−1 after 30 minutes and a turnover number (TON) of 120.
A Supramolecular Strategy for the Synthesis of Cyclic Oligomers and Polymers by Ring Expansion
Authors: Duss Michael , Soto Miguel A. , Patrick Brian O. , MacLachlan Mark J. 
Published: August 2022

AbstractRing‐opening metathesis polymerization (ROMP) of strained macrocycles is a key method to prepare diverse polymers. However, lack of ring strain in most macrocycles is an impediment to polymerization. In this paper, the polymerization/oligomerization of unstrained macrocycles was achieved using a supramolecular approach, leading selectively to cyclic products. Diphenyl thiourea and other guest molecules were used as additives to the ROMP reaction of unstrained macrocycles. An intermediate host‐guest complex leads to the stabilization of the open form of the macrocycle after treatment with Grubbs catalysts, thereby favoring polymerization by inhibiting the ring‐closing reaction back to the monomer. This proof‐of‐concept enables ring‐expansion polymerization of unstrained macrocycles leading to cyclic polymers with molecular weights up to 6700 Da.
Photocatalytic Reduction of Nicotinamide Co‐factor by Perylene Sensitized RhIII Complexes**
Authors: Brückmann Jannik , Müller Carolin , Friedländer Ilse , Mengele Alexander K. , Peneva Kalina , Dietzek‐Ivanšić Benjamin , Rau Sven 
Published: August 2022

AbstractThe ambitious goal of artificial photosynthesis is to develop active systems that mimic nature and use light to split water into hydrogen and oxygen. Intramolecular design concepts are particularly promising. Herein, we firstly present an intramolecular photocatalyst integrating a perylene‐based light‐harvesting moiety and a catalytic rhodium center (RhIIIphenPer). The excited‐state dynamics were investigated by means of steady‐state and time‐resolved absorption and emission spectroscopy. The studies reveal that photoexcitation of RhIIIphenPer yields the formation of a charge‐separated intermediate, namely RhIIphenPer⋅+, that results in a catalytically active species in the presence of protons.
π‐Extended Pleiadienes by [5+2] Annulation of 1‐Boraphenalenes and ortho‐Dihaloarenes
Authors: Schnitzlein Matthias , Zhu Chongwei , Shoyama Kazutaka , Würthner Frank 
Published: August 2022

AbstractPalladium‐catalyzed [5+2] annulation of 1‐boraphenalenes with ortho‐dihaloarenes afforded negatively curved π‐extended pleiadienes. Two benzo[1,2‐i:4,5‐i’]dipleiadienes (BDPs) featuring a seven‐six‐seven‐membered ring arrangement were synthesized and investigated. Their crystal structure revealed a unique packing arrangement and theoretical calculations were employed to shed light onto the dynamic behavior of the BDP moiety and its aromaticity. Further, a naphthalene‐fused pleiadiene was stitched together by oxidative cyclodehydrogenation to yield an additional five‐membered ring. This formal azulene moiety led to distinct changes in optical and redox properties and increased perturbation of the aromatic system.