bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of software enabling Chinese medicine-based precision treatment for osteoporosis at the gene and pathway levels
Authors: Li Jinyu , Feng Guiyu , He Haoyang , Wang Haolin , Tang Jia , Han Aiqing , Mu Xiaohong , Zhu Weifeng 
Published: April 2022

Abstract
Background
Precision medicine aims to address the demand for precise therapy at the gene and pathway levels. We aimed to design software to allow precise treatment of osteoporosis (OP) with Chinese medicines (CMs) at the gene and pathway levels.

Methods
PubMed, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI), China Science and Technology Journal Database (VIP database), and the Wanfang database were searched to identify studies treating osteoporosis with CMs. The TCMSP was used to identify bioactive ingredients and related genes for each CM. Gene expression omnibus (GEO) database and the limma package were used to identify differentially expressed genes in osteoporosis. Perl software was used to identify the shared genes between the bioactive components in CM and osteoporosis. R packages and bioconductor packages were used to define the target relationship between shared genes and their related pathways. Third-party Python libraries were used to write program codes. Pyinstaller library was used to create an executable program file.

Results
Data mining: a total of 164 CMs were included, but Drynariae Rhizoma (gusuibu) was used to present this process. We obtained 44 precise relationships among the bioactive ingredients of Drynariae Rhizoma, shared genes, and pathways. Python programming: we developed the software to show the precise relationship among bioactive ingredients, shared genes, and pathways for each CM, including Drynariae Rhizoma.

Conclusions
This study could increase the precision of CM, and could provide a valuable and convenient software for searching precise relationships among bioactive ingredients, shared genes, and pathways.
Knowledge and attitudes towards clinical trials among women with ovarian cancer: results of the ACTO study
Authors: Mosconi Paola , Roberto Anna , Cerana Nicoletta , Colombo Nicoletta , Didier Florence , D’Incalci Maurizio , Lorusso Domenica , Peccatori Fedro Alessandro ,   Network of Clinicians and Participants (1), Artioli Grazia , Cavanna Luigi , Ceccherini Rita , Cirigliano Giovanna , Comerci Giuseppe , Cormio Gennaro , Crippa Alessandra , Farolfi Alberto , Febbraro Antonio , Giardina Donatella , Greggi Stefano , Lalle Maurizio , Lapresa Mariateresa , Marzola Marina , Merisio Carla , Mosconi Anna Maria , Peiretti Michele , Ricci Giuseppe , Ronzino Graziana , Scambia Giovanni , Scollo Paolo , Sina Federica , Stella Giulia Carlo , Tomao Federica , Vici Patrizia , Zola Paolo 
Published: April 2022

Abstract
Background
Despite several initiatives by research groups, regulatory authorities, and scientific associations to engage citizens/patients in clinical research, there are still obstacles to participation. Among the main discouraging aspects are incomplete understanding of the concepts related to a clinical trial, and the scant, sometimes confused, explanations given. This observational, cross-sectional multicenter study investigated knowledge, attitudes and trust in clinical research.
We conducted a survey among women with ovarian cancer at their first follow-up visit or first therapy session, treated in centers belonging to the Mario Negri Gynecologic Oncology (MaNGO) and Multicenter Italian Trials in Ovarian Cancer (MITO) groups. A questionnaire on knowledge, attitudes and experience was assembled ad hoc after a literature review and a validation process involving patients of the Alliance against Ovarian Cancer (ACTO).

Results
From 25 centers 348 questionnaire were collected; 73.5% of responders were 56 years or older, 54.8% had a high level of education, more than 80% had no experience of trial participation. Among participants 59% knew what clinical trials were and 71% what informed consent was. However, more than half did not know the meaning of the term randomization. More than half (56%) were in favor of participating in a clinical trial, but 35% were not certain. Almost all responders acknowledged the doctor’s importance in decision-making. Patients’ associations were recognized as having a powerful role in the design and planning of clinical trials.

Conclusions
This study helps depict the knowledge and attitudes of women with ovarian cancer in relation to clinical trials, suggesting measures aimed at improving trial “culture”, literacy and compliance, and fresh ways of communication between doctors and patients.
CMMRD caused by PMS1 mutation in a sudanese consanguineous family
Authors: Hamad Reem S. , Ibrahim Muntaser E. 
Published: April 2022

AbstractA consanguineous family of three siblings presented with different early onset pediatric cancers. Whole-exome sequencing of parents DNA revealed a deleterious frameshift mutation in hPMS1 the first to be reported in association to a CMMRD phenotype.
Effect of Baihu and Guizhi decoction in acute gouty arthritis: study protocol for a randomized controlled trial
Authors: He Yikun , Dai Chaoran , Shen Jiaying , Chen Qianwen , Gao Jiandong , Pan Xin , Gan Jing 
Published: April 2022

Abstract
Background
The prevalence rates of gout worldwide have increased annually. Acute gouty arthritis (AGA) accounts for a large proportion of gout patients and causes severe physical and mental pain in patients. Controlling the occurrence and development of gout inflammation is the first step in the treatment of gout. The main treatment drugs in gout are non-steroid anti-inflammatory drugs (NSAIDs), colchicine, and glucocorticoids, but these treatments have many adverse reactions which limit their clinical application. Baihu and Guizhi decoction (BHGZ) is one of the classic prescriptions in the Synopsis of the Golden Chamber and is a good prescription for AGA. Previous clinical studies have shown that BHGZ confers a strong benefit for treating AGA. However, the literature shows a lack of high-quality RCT research on BHGZ with respect to AGA. Therefore, in this study, we use a randomized, double-blind, controlled study with a placebo to evaluate the clinical efficacy and safety of BHGZ on the AGA of moist heat arthralgia spasm syndrome.

Methods
This study is a randomized, double-blind, controlled clinical trial. A total of 102 adult participants with AGA of moist heat arthralgia spasm syndrome will be enrolled, with balanced treatment allocation (1:1). The experimental intervention will be BHGZ plus the low-dose colchicine, and the control intervention will be placebo plus the low-dose colchicine for 10 days. To study the clinical efficacy (including VAS score; joint tenderness, joint swelling, joint movement disorder; TCM evidence efficacy score) and the changes of inflammatory indexes. At the same time, the improvement of joint inflammation in patients with AGA will be observed from musculoskeletal ultrasound imaging, and the safety evaluation will be carried out.

Discussion
This study will be the first placebo-controlled RCT to assess whether BHGZ plus low-dose colchicine have beneficial effects on changing reducing inflammation of joints for patients with AGA of moist heat arthralgia spasm syndrome. The results of this trial will help to provide evidence-based recommendations for clinicians.

Trial registration
Chinese Clinical Trials Register ChiCTR1900024974. Registered on 5 August 2019
A randomized sham-controlled trial on the effects of dual-tDCS “during” physical therapy on lower limb performance in sub-acute stroke and a comparison to the previous study using a “before” stimulation protocol
Authors: Klomjai Wanalee , Aneksan Benchaporn 
Published: April 2022

Abstract
Background
Dual-transcranial direct current stimulation (tDCS) has been used to rebalance the cortical excitability of both hemispheres following unilateral-stroke. Our previous study showed a positive effect from a single-session of dual-tDCS applied before physical therapy (PT) on lower limb performance. However, it is still undetermined if other timings of brain stimulation (i.e., during motor practice) induce better effects. The objective of this study was to examine the effect of a single-session of dual-tDCS “during” PT on lower limb performance in sub-acute stroke and then compare the results with our previous data using a “before” stimulation paradigm.

Method
For the current “during” protocol, 19 participants were participated in a randomized sham-controlled crossover trial. Dual-tDCS over the M1 of both cortices (2 mA) was applied during the first 20 min of PT. The Timed Up and Go and Five-Times-Sit-To-Stand tests were assessed at pre- and post-intervention and 1-week follow-up. Then, data from the current study were compared with those of the previous “before” study performed in a different group of 19 subjects. Both studies were compared by the difference of mean changes from the baseline.

Results
Dual-tDCS “during” PT and the sham group did not significantly improve lower limb performance. By comparing with the previous data, performance in the “before” group was significantly greater than in the “during” and sham groups at post-intervention, while at follow-up the “before” group had better improvement than sham, but not greater than the “during” group.

Conclusion
A single-session of dual-tDCS during PT induced no additional advantage on lower limb performance. The “before” group seemed to induce better acute effects; however, the benefits of the after-effects on motor learning for both stimulation protocols were probably not different.
Trial registration Current randomized controlled trials was prospectively registered at the clinicaltrials.gov, registration number: NCT04051671. The date of registration was 09/08/2019.
Effect of an acute exercise on early responses of iron and iron regulatory proteins in young female basketball players
Authors: Cichoń Justyna , Ostapiuk-Karolczuk Joanna , Cieślicka Mirosława , Dziewiecka Hanna , Marcinkiewicz Anita , Tafil-Klawe Małgorzata , Basta Piotr , Maciejewski Dariusz , Skarpańska-Stejnborn Anna 
Published: April 2022

Abstract
Background
The accumulation of physiological stress and the presence of inflammation disturb iron management in athletes during intense training. However, little is known about the mechanisms regulating iron levels in athletes during training periods with low training loads. In the current study, we analyzed the effect of an acute exercise on early responses of iron and iron regulatory proteins at the end of such training periods.

Methods
The study was performed at the end of competitive phase of training. A total of 27 trained female basketball players were included in the study after application of the inclusion/exclusion criteria. The participants performed an incremental exercise on a treadmill. Blood samples were taken before the test, immediately after exercise, and after 3 h of restitution. Parameters, such as interleukin (IL) 6, hepcidin, ferritin, transferrin, hemopexin, and lactoferrin levels, total iron-biding capacity (TIBC), unsaturated iron-biding capacity (UIBC) were determined by using appropriate biochemical tests.

Results
The level of iron increased significantly after exercise, and then decreased within next 3 h restitution. Except for iron levels, only TIBC levels significantly increased after exercise and decreased to baseline level during rest period. No significant changes in the levels of hepcidin, IL-6, and other proteins related to the iron homeostasis were observed.

Conclusions
The increases in iron level after acute exercise is short-term and transient and appear to have been insufficient to induce the acute systemic effects in rested athletes.
Simultaneous biodegradation of λ-cyhalothrin pesticide and Vicia faba growth promotion under greenhouse conditions
Authors: Abdelkader Aisha A. , Khalil Mary S. , Mohamed Mahmoud S. M. 
Published: April 2022

Abstractλ-cyhalothrin is a widely used synthetic pyrethroid insecticide and its persistence in plant, soil and water exerts a detrimental effect on humans as well as the environment. There are many studies regarding isolated bacteria capable of degrading λ-cyhalothrin in vitro. However, limited work has been done examining the microbial degradation of λ-cyhalothrin together with plant growth promotion under greenhouse conditions. In this study, 43 bacterial strains were isolated from heavily polluted soil with λ-cyhalothrin by the enrichment technique. The plant growth promotion characteristics of all isolates were evaluated. The results revealed that five isolates were potential in λ-cyhalothrin biodegradation at high concentration (1200 mg/L) within only 24 h together with their high plant growth promotion abilities. The morphological, biochemical and 16S rDNA sequence analyses identified the isolates as Bacillus subtilis strains. The GC/MS analysis revealed that the selected isolates reached high levels of degradation after only two days, the degradation percentage ranged from 95.72 to 99.52% after 48 h of incubation. Furthermore, the degradation pathway for complete detoxification and metabolism of λ-cyhalothrin was established. Moreover, greenhouse experiment was conducted, the results indicate that the application of seed coat significantly enhanced Vicia faba seedling growth and caused an increase from 38.4 to 40.2% percentage of fresh and dry weight, respectively compared to untreated control. All isolates were effective to remove the pesticide residues in Vicia faba seedlings and recorded the highest degradation percentage of 83.79 under greenhouse conditions. Therefore, it can be concluded that the Bacillus subtilis strains isolated in this study have a dual potential role in complete mineralization of λ-cyhalothrin residues in vivo as well as effective biofertilization for future use in sustainable agriculture.
Potential of the microalgae Chlorella fusca (Trebouxiophyceae, Chlorophyta) for biomass production and urban wastewater phycoremediation
Authors: Arrojo María Ángeles , Regaldo Luciana , Calvo Orquín Jesús , Figueroa Félix L. , Abdala Díaz Roberto Teófilo 
Published: April 2022

Abstract
The present work focuses on: (1) the evaluation of the potential of Chlorella fusca to grow and synthesize metabolites of biotechnological interest, after being exposed for fourteen days to urban wastewater (UW) from Malaga city (UW concentrations: 25%, 50%, 75%, and 100%); (2) the study of the capacity of C. fusca to bioremediate UW in photobioreactors at laboratory scale; and (3) the evaluation of the effect of UW on the physiological status of C. fusca, as photosynthetic capacity by using in vivo Chl a fluorescence related to photosystem II and the production of photosynthetic pigments. C. fusca cell density increased in treatments with 50% UW concentration, followed by the treatment with 100% UW, 75% UW, the control, and finally 25% UW. Protein content increased to 50.5% in 75% UW concentration. Stress induced to microalgal cultures favored the increase of lipid production, reaching a maximum of 16.7% in 100% UW concentration. The biological oxygen demand (BOD5) analysis indicated a 75% decrease in 100% UW concentration. Dissolved organic carbon (DOC) levels decreased by 41% and 40% in 50% UW and 100% UW concentration, and total nitrogen (TN) decreased by 55% in 50% UW concentration. The physiological status showed the stressful effect caused by the presence of UW on photosynthetic activity, with increasing impact as UW concentration grew. In the framework of circular economy, we seek to deepen this study to use the biomass of C. fusca to obtain metabolites of interest for biofuel production and other biotechnological areas.

Graphical Abstract
Precious blood and nourishing offal: past and present slaughtering perspectives in Sámi reindeer pastoralism
Authors: Sara Ravdna Biret Marja E. , Syse Karen Lykke , Mathiesen Svein Disch 
Published: April 2022

AbstractIn the Arctic, indigenous reindeer herding peoples rely on a pastoralist food and knowledge system that supplies them with protein, vitamins, and minerals. Reindeer pastoralism is a product of the interaction between animals’ physical needs, their behaviour, and the skills of the herders. The food systems of Sámi reindeer pastoralists depend on indigenous knowledge about mountain slaughtering. When the first stationary reindeer slaughterhouse opened in Guovdageaidnu (Northern Norway) in 1957, rationalisation of reindeer husbandry and methods of reindeer slaughter took place. Animal welfare and reindeer slaughter within slaughterhouses are well-documented in Norway; the historical knowledge about slaughtering reindeer in the mountains, however, is barely documented and is in danger of being lost. A qualitative study entailing interviews with five Sámi reindeer herders (50–80 years old) from Guovdageaidnu and Varanger explains indigenous, nomadic methods of killing and slaughtering reindeer. The traditional Sámi way of killing reindeer (Sámi:giehtadit) was to pierce the heart with a large knife through the chest (Sámi:mielga), particularly in the pastures close to where the herd grazed to avoid distressing the reindeer before taking their lives. This allowed reindeer herders to use the whole reindeer for food, unlike the practice in stationary slaughterhouses, which merely utilises select muscles for human consumption. Although the Supreme Court of Norway in 2008 ruled that traditional slaughter practice without stunning was illegal, this historical account provides evidence that thegiehtaditmethod was a rational way to kill a reindeer, as bleeding (haemorrhage) in the thorax offers both high-quality blood, offal, and meat for human consumption. We conclude that the traditional Sámi method is based on systematic, complex, and holistic indigenous knowledge and determines the foods reindeer herders eat.
Reindeer herding statistics in Russia: issues of reliability, interpretation, and political effect
Authors: Istomin Kirill V. , Laptander Roza I. , Habeck Joachim Otto 
Published: April 2022

AbstractOfficial reindeer herding statistics are an invaluable source of data for both social and natural scientists wishing to understand and model ecological systems in the Arctic. However, as with all official statistics, reindeer herding statistics are subject to certain distortions emerging from the way they are collected and processed as well as from a priori assumptions. In this article, we analyse Soviet/Russian reindeer herding statistics in order to reveal these distortions and assumptions and show how these statistics should be interpreted. Particularly, we analyse reindeer ownership categories and reindeer age/sex categories, spatial organization of the data, so-called magical numbers (statistical parameters used by the state to assess the quality of reindeer herding management), and the manner of collecting statistics. We show that official Soviet/Russian statistics reflected the world as the state wanted to see it, even if it obviously did not completely correspond to the world ‘out there’. In Soviet times, the state even made systematic attempts to change this world to better correspond to the statistics, which, however, was never fully achieved. On the basis of this analysis, we offer some recommendations for how an interested researcher should read and understand Russian reindeer herding statistics.
Crystal structure of the Ilheus virus helicase: implications for enzyme function and drug design
Authors: Wang De-Ping , Wang Mei-Yue , Li Yong-Mei , Shu Wen , Cui Wen , Jiang Fang-Ying , Zhou Xin , Wang Wen-Ming , Cao Ji-Min 
Published: April 2022

AbstractBackgroundThe Ilheus virus (ILHV) is an encephalitis associated arthropod-borne flavivirus. It was first identified in Ilheus City in the northeast Brazil before spreading to a wider geographic range. No specific vaccines or drugs are currently available for the treatment of ILHV infections. The ILHV helicase, like other flavivirus helicases, possesses 5ʹ-triphosphatase activity. This allows it to perform ATP hydrolysis to generate energy as well as sustain double-stranded RNA’s unwinding during ILHV genome replication. Thus, ILHV helicase is an ideal target for inhibitor design.ResultsWe determined the crystal structure of the ILHV helicase at 1.75-Å resolution. We then conducted molecular docking of ATP-Mn2+to the ILHV helicase. Comparisons with related flavivirus helicases indicated that both the NTP and the RNA-ILHV helicase binding sites were conserved across intra-genus species. This suggested that ILHV helicase adopts an identical mode in recognizing ATP/Mn2+. However, the P-loop in the active site showed a distinctive conformation; reflecting a different local structural rearrangement. ILHV helicase enzymatic activity was also characterized. This was found to be relatively lower than that of the DENV, ZIKV, MVE, and ALSV helicases. Our structure-guided mutagenesis revealed that R26A, E110A, and Q280A greatly reduced the ATPase activities. Moreover, we docked two small molecule inhibitors of DENV helicase (ST-610 and suramin) to the ILHV helicase and found that these two molecules had the potential to inhibit the activity of ILHV helicase as well.ConclusionHigh-resolution ILHV helicase structural analysis demonstrates the key amino acids of ATPase activities and could be useful for the design of inhibitors targeting the helicase of ILHV.
Mapping evidence on the use of health promotion and disease prevention interventions as a strategy to sustaining pro-poor health insurance schemes: a scoping review protocol
Authors: Inkoom Loretta , Ansu-Mensah Monica , Bawontuo Vitalis , Kuupiel Desmond 
Published: April 2022

Abstract
Background
Removing financial barriers and making healthcare accessible to all who need it remains an essential component of the United Nations’ sustainable development goals. Pro-poor healthcare financing schemes are policies that enable patients to concentrate on obtaining absolute medical care when needed rather than worrying about the cost of care. The demand for health services in healthcare facilities has increased tremendously due to the increasing burden of communicable and non-communicable diseases. This potentially threatens the sustainability of pro-poor health financing schemes. This study seeks to synthesize literature and map evidence on the use of health promotion and disease prevention interventions as a strategy to sustaining pro-poor health financing schemes globally.

Methods
We will conduct a systematic scoping review utilizing the Arksey and O’Malley framework, Levac et al. recommendations, and the Joanna Briggs Institute guidelines. A comprehensive keyword search for relevant published articles will be conducted in MEDLINE through PubMed, Web of Science, Google Scholar, SCOPUS, CINAHL, and Science Direct from 1 January 2000 to the last search date in 2021. Limiters such as date and language (English) will be applied, but study design limitations will be removed during the search. Boolean term AND/OR Medical Subject Heading terms will also be included. The reference list of all included articles will also be searched for potentially eligible articles. Two investigators will independently screen the articles in parallel at the abstract and full-text stages using the eligibility criteria designed in a Google form. Charting of data will also be conducted independently by two investigators using a piloted data abstraction form and thematic analysis conducted. The emerging themes will be collated, summarized, and the results reported.

Discussion
We hope to provide evidence of diverse health promotion and disease prevention policies/strategies used by countries to sustain their pro-poor health financing schemes for possible adoption by other countries. We also anticipate finding research gaps for further studies to help find innovative contextualized health prevention and promotion strategies to sustain pro-poor health financing schemes especially those in LMICs. The findings will be comprehensively discussed and disseminated at conferences and publication in a peer-reviewed journal.