bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glioblastoma Recurrence Versus Radiotherapy Injury
Authors: Dang Haodan , Zhang Jinming , Wang Ruimin , Liu Jiajin , Fu Huaping , Lin Mu , Xu Baixuan 
Published: April 2022

Purpose
To evaluate the diagnostic potential of decision-tree model of diffusion kurtosis imaging (DKI) and 11C-methionine (11C-MET) PET, for the differentiation of radiotherapy (RT) injury from glioblastoma recurrence.

Methods
Eighty-six glioblastoma cases with suspected lesions after RT were retrospectively enrolled. Based on histopathology or follow-up, 48 patients were diagnosed with local glioblastoma recurrence, and 38 patients had RT injury between April 2014 and December 2019. All the patients underwent PET/MRI examinations. Multiple parameters were derived based on the ratio of tumor to normal control (TNR), including SUVmax and SUVmean, mean value of kurtosis and diffusivity (MK, MD) from DKI, and histogram parameters. The diagnostic models were established by decision trees. Receiver operating characteristic analysis was used for evaluating the diagnostic accuracy of each independent parameter and all the diagnostic models.

Results
The intercluster correlations of DKI, PET, and texture parameters were relatively weak, whereas the intracluster correlations were strong. Compared with models of DKI alone (sensitivity =1.00, specificity = 0.70, area under the curve [AUC] = 0.85) and PET alone (sensitivity = 0.83, specificity = 0.90, AUC = 0.89), the combined model demonstrated the best diagnostic accuracy (sensitivity = 1.00, specificity = 0.90, AUC = 0.95).

Conclusions
Diffusion kurtosis imaging, 11C-MET PET, and histogram parameters provide complementary information about tissue. The decision-tree model combined with these parameters has the potential to further increase diagnostic accuracy for the discrimination between RT injury and glioblastoma recurrence over the standard Response Assessment in Neuro-Oncology criteria. 11C-MET PET/MRI may thus contribute to the management of glioblastoma patients with suspected lesions after RT.
68Ga-DOTATATE Uptake in Well-Differentiated Neuroendocrine Tumor of the External Auditory Canal
Authors: Erol Fenercioğlu Özge , Beyhan Ediz , Şahin Rahime , Baloğlu Mehmet Can , Çermik Tevfik Fikret 
Published: April 2022

Abstract
A 38-year-old woman presented with a 4-month history of left-sided otalgia and otorrhea. Otoscopy revealed a mass in the left external auditory canal causing obliteration. A biopsy was taken, and the diagnosis was consistent with a well-differentiated neuroendocrine tumor. The patient was referred to our department for staging with 68Ga-DOTATATE PET/CT. Increased radiopharmaceutical uptake was observed in the primary tumor and metastatic lymph nodes.
Vas Deferens and Inguinal Canal Metastasis of Prostate Cancer Revealed by 68Ga-Prostate-Specific Membrane Antigen PET/CT
Authors: Erol Fenercioğlu Özge , Alçin Göksel , Arslan Esra , Çermik Tevfik Fikret , Ergül Nurhan 
Published: April 2022

Abstract
A 71-year-old man with prostate adenocarcinoma underwent 68Ga–prostate-specific membrane antigen (PSMA) PET/CT for staging. 68Ga-PSMA PET/CT showed the primary lesion along with bladder, rectum, bilateral seminal vesicle invasion, and metastatic pelvic lymph nodes with intense 68Ga-PSMA uptake. Also, PET/CT showed rarely seen bilateral vas deferens invasion and metastasis to the inguinal canal. These rare metastases may be indicative of poor biological behavior and prognosis.
Rechallenge With Additional Doses of 177Lu-DOTATOC After Failure of Maintenance Therapy With Cold Somatostatin Analogs
Authors: Weich Alexander , Werner Rudolf A. , Serfling Sebastian E. , Solnes Lilja B. , Pomper Martin G. , Buck Andreas K. , Higuchi Takahiro , Rowe Steven P. 
Published: April 2022

Abstract
Here, we report a case of a 52-year-old woman with a well-differentiated, metastasized neuroendocrine tumor (NET G1) of the duodenum. Initial imaging with 68Ga-DOTATOC revealed multiple sites of disease with intense uptake. Peptide receptor radionuclide therapy (PRRT) with 177Lu-DOTATOC induced partial remission. Treatment was then switched to cold somatostatin analog as a maintenance therapy. After 2 years of follow-up, progressive disease with multiple lesions in the skeleton was noted. Given the initial response to PRRT, a rechallenge with another 2 cycles of PRRT were given, and the patient had an excellent response to treatment, in particular in the skeleton.
Dual-Phase 18F-FP-CIT PET in 2 Different Clinical Phenotypes of Sporadic Creutzfeldt-Jakob Disease
Authors: Lee Keun , Park Dong Gueu , Kim Min Seung , An Young Sil , Yoon Jung Han 
Published: April 2022

Abstract
Early diagnosis of Creutzfeldt-Jakob disease (CJD) patients is often challenging due to the low sensitivity of the current clinical diagnostic criteria. We describe MRI and dual-phase 18F-FP-CIT PET findings in 2 cases of sporadic CJD presenting different clinical phenotypes (Heidenhain variant and corticobasal syndrome). Our case series suggest that dual-phase FP-CIT-PET findings may improve the diagnosis of CJD by combining the perfusion patterns in early phase with the dopamine transporter density in delayed phase. Familiarity with these dual-phase FP-CIT PET findings is helpful for early correct diagnosis of CJD.
Plasma biomarkers for diagnosis of Alzheimer’s disease and prediction of cognitive decline in individuals with mild cognitive impairment
Authors: Kivisäkk Pia , Sweeney Thadryan , Carlyle Becky C. , Trombetta Bianca A. , LaCasse Kathryn , El-Mufti Leena , Tuncali Idil , Chibnik Lori B. , Das Sudeshna , Scherzer Clemens R. , Johnson Keith A. , Dickerson Bradford C. , Gomez-Isla Teresa , Blacker Deborah , Oakley Derek H. , Frosch Matthew P. , Hyman Bradley T. , Aghvanyan Anahit , Bathala Pradeepthi , Campbell Christopher , Sigal George , Stengelin Martin , Arnold Steven E. 
Published: April 2022

ABSTRACTBackgroundThe last few years have seen major advances in blood biomarkers for Alzheimer’s Disease (AD) with the development of ultrasensitive immunoassays, promising to transform how we diagnose, prognose, and track progression of neurodegenerative dementias.MethodsWe evaluated a panel of four novel ultrasensitive electrochemiluminescence (ECL) immunoassays against presumed CNS derived proteins of interest in AD in plasma [phosphorylated-Tau181 (pTau181), total Tau (tTau), neurofilament light (NfL), and glial fibrillary acidic protein (GFAP)]. 366 plasma samples from the Massachusetts Alzheimer’s Disease Research Center’s longitudinal cohort study were examined to differentiate definite AD, other neurodegenerative diseases (OND), and cognitively normal (CN) individuals. A subset of samples were selected to have longitudinal follow up to also determine the utility of this plasma biomarker panel in predicting 4-year risk for cognitive decline in individuals with different levels of cognitive impairment.ResultspTau181, tTau and GFAP were higher in AD compared to CN and OND, while NfL was elevated in AD and further increased in OND. pTau181 performed the best (AD vs CN: AUC=0.88, 2-fold increase; AD vs OND: AUC=0.78, 1.5-fold increase) but tTau also showed excellent discrimination (AD vs CN: AUC=0.79, 1.5-fold increase; AD vs OND: AUC=0.72, 1.3-fold increase). Participants with MCI who progressed to AD dementia had higher baseline plasma concentrations of pTau181, NfL, and GFAP compared to non-progressors with the best discrimination for pTau181 (AUC=0.82, 1.7-fold increase) and GFAP (AUC=0.81, 1.6-fold increase).ConclusionsThese new ultrasensitive ECL plasma assays for pTau181, tTau, NfL, and GFAP detect CNS disease with high specificity and accuracy. Moreover, the absolute baseline plasma levels of pTau and GFAP reflect clinical disease aggressiveness over the next 4 years, providing diagnostic and prognostic information that may have utility in both clinical and clinical trial populations.Classification of EvidenceThis study provides Class II evidence that plasma levels of pTau181, tTau, NfL, and GFAP are associated with AD and that pTau181 and GFAP are associated with progression from MCI to AD dementia.
Validation of Reduced S-gene Target Performance and Failure for Rapid Surveillance of SARS-CoV-2 Variants
Authors: Clark Cyndi , Schrecker Joshua , Hardison Matthew , Taitel Michael S. 
Published: April 2022

AbstractSARS-CoV-2, the virus that causes COVID-19, has many variants capable of rapid transmission causing serious illness. Timely surveillance of new variants is essential for an effective public health response. Ensuring availability and access to diagnostic and molecular testing is key to this type of surveillance. This study utilized reverse transcription polymerase chain reaction (RT-PCR) and whole genome sequencing results from COVID-19-positive patient samples obtained through a collaboration between Aegis Sciences Corporation and Walgreens Pharmacy that has conducted more than 8.5 million COVID-19 tests at ∼5,200 locations across the United States and Puerto Rico.Viral evolution of SARS-CoV-2 can lead to mutations in the S-gene that cause reduced or failed S-gene amplification in diagnostic PCR tests. These anomalies, labeled reduced S-gene target performance (rSGTP) and S-gene target failure (SGTF), are characteristic of Alpha and Omicron (B.1.1.529, BA.1, and BA.1.1) lineages. This observation has been validated by whole genome sequencing and can provide presumptive lineage data following completion of diagnostic PCR testing in 24-48 hours from collection. Active surveillance of trends in PCR and sequencing results is key to the identification of changes in viral transmission and emerging variants. This study shows that rSGTP and SGTF can be utilized for near real-time tracking and surveillance of SARS-CoV-2 variants.
Rasch Validation of the Warwick-Edinburgh Mental Well-being Scale (WEMWBS) in Community-Dwelling Adults and in Adults with Stroke in the US
Authors: Deng Wei , Carpentier Sydney , Blackwood Jena , Van de Winckel Ann 
Published: April 2022

AbstractBackgroundWith the recent ongoing global COVID-19 pandemic and political divide in the United States (US), there is an urgent need to address the soaring mental well-being problems and to promote positive well-being. The Warwick-Edinburgh Mental Well-being Scale (WEMWBS) measures the positive aspects of mental health. Previous studies confirmed its construct validity, reliability, and unidimensionality with confirmatory factor analysis. Four studies have performed a Rasch analysis on the WEMWBS, but none of them tested adults in the US. The goals of our study are to use Rasch analysis to validate the WEMWBS in the general US population and in adults with stroke.MethodsWe recruited community-dwelling adults and adults with chronic stroke with upper limb hemiplegia or hemiparesis. We used the Rasch Unidimensional Measurement Model (RUMM) 2030 software to evaluate item and person fit, targeting, person separation reliability (PSR), and differential item functioning (DIF) for sample sizes of at least 200 persons in each subgroup.ResultsAfter deleting two items, the WEMBS analyzed in our 553 community-dwelling adults (average age 51.22±17.18 years; 358 women) showed an excellent PSR=0.91 as well as person and item fit, but the items are too easy for this population (person mean location=2.17±2.00). There was no DIF for sex, mental health, or practicing breathing exercises. In the 37 adults with chronic stroke (average age 58±13; 11 women) the WEMWBS had a good item and person fit, and PSR=0.92, but the items were too easy for this group as well (person mean location=3.13±2.00).ConclusionsThe WEMWBS had good item and person fit but the targeting is off when used in community-dwelling adults and adults with stroke in the US. Adding more difficult items might improve the targeting and capture a broader range of positive mental wellbeing in both populations. Our pilot data in adults with stroke needs to be confirmed in a larger sample size.
Elucidating host cell response pathways and repurposing therapeutics for SARS-CoV-2 and other coronaviruses using gene expression profiles of chemical and genetic perturbations
Authors: Shen Zhewei , Halberg Anna , Fong Jia Yi , Guo Jingyu , Song Gavin , Louie Brent , Luedtke Gregory R. , Visuthikraisee Viwat , Protter Andy , Koh Xiaoying , Baik Taegon , Lum Pek Yee 
Published: April 2022

AbstractCOVID-19 is an ongoing pandemic that has been causing devastation across the globe for over 2 years. Although there are multiple vaccines that can prevent severe symptoms, effective COVID-19 therapeutics are still of importance. Using our proprietary in silico SMarTR™ engine, we screened more than 22,000 unique compounds represented by over half a million gene expression profiles to uncover compounds that can be repurposed for SARS-CoV-2 and other coronaviruses in a timely and cost-efficient manner. We then tested 13 compounds in vitro and found three with potency against SARS-CoV-2 with reasonable cytotoxicity. Bortezomib and homoharringtonine are some of the most promising hits with IC50 of 1.39 μM and 0.16 μM, respectively for SARS-CoV-2. Tanespimycin and homoharringtonine were effective against the common cold coronaviruses. In-depth analysis highlighted proteasome, ribosome, and heat shock pathways as key targets in modulating host responses during viral infection. Further studies of these pathways and compounds have provided novel and impactful insights into SARS-CoV-2 biology and host responses that could be further leveraged for COVID-19 therapeutics development.
System Integrated Digital Empowerment and Rehabilitation to promote patient Activation and well-Being (SIDERA^B): Protocol for a Randomized Crossover Trial on Effectiveness and Implementation
Authors: Rossetto Federica , Borgnis Francesca , Blasi Valeria , Banfi Paolo Innocente , Tavanelli Monica , Realdon Olivia , Mantovani Fabrizia , Foglia Emanuela , Garagiola Elisabetta , Croce Davide , Nalin Marco , Baglio Francesca 
Published: April 2022

AbstractCONTEXTthe current increasing demand for rehabilitation among people with Non-Communicable Diseases (NCDs) requires the identification of home-based digital solutions alternative to conventional in-clinic interventions.OBJECTIVEthis protocol proposes to test the effectiveness of an individualized telerehabilitation platform (SIDERA^B), with respect to the traditional face-to-face rehabilitation, in ensuring the continuity of care in patients with NCDs.DESIGN, SETTING, AND SUBJECTSthis randomized, single-blind, controlled two-period crossover trial will involve about 150 outpatients with NCDs (N=40 with Chronic Heart Failure – CHF, N=60 with Chronic Obstructive Pulmonary Disease – COPD, and N=50 with Parkinson’s Disease – PD) from the rehabilitation units of IRCCS Fondazione Don Carlo Gnocchi of Milan. Each participant will experience, consequently, two different types of interventions: rehabilitation with the SIDERA^B system (SIDERA^B – S), which allow for both tele-rehabilitation activities and tele-monitoring of vital parameters, and rehabilitation as usual (Usual Care – U) including a manual of rehabilitative exercises and self-monitoring of vital parameters.INTERVENTIONSsubjects will be randomly assigned to one of the two specified sequences of interventions: U/S/U (the USU group), and S/U (the SU group). Both groups will be assessed at the baseline (T1), after the first intervention (T2), and after the second intervention (T3), with a follow-up evaluation (T4) scheduled only for the USU group.MAIN OUTCOME MEASURESa multifaceted evaluation including quality of life and clinical/functional measures will be conducted at each time-point of assessment. The primary outcome measures will be 1) change in activation of patients measured by the Patient Activation Measure scale, and 2) change in subject’s level of activity and participation measured by the WHO Disability Assessment Schedule 2.0.CONCLUSIONSIDERA^B could represent a promising innovative digital solution able to support the ongoing migration of rehabilitation care from the clinic to the patient’s home, for the optimal long-term management of NCDs.Trial registrationThe SIDERA^B trial was registered in the clinicaltrials.gov database (identifier NCT04041193) on August 1, 2019.
helixCAM: A Platform for Programmable Cellular Assembly in Bacteria and Human Cells
Authors: Chao George , Wannier Timothy M. , Travis Clair , Borders Nathaniel C. , Appleton Evan , Chadha Anjali , Lebar Tina , Church George M. 
Published: April 2022

SummaryInteractions between cells are indispensable for creating structure and signaling. The ability to direct precise cell-cell interactions would be powerful for engineering tissues, understanding signaling pathways, and directing immune cell targeting. In humans, intercellular interactions are mediated by cell adhesion molecules (CAMs). However, CAMs are natively expressed by many cells and have cross-reactivity, making them unsuitable for programming specific interactions. Here, we showcase “helixCAM,” a platform for engineering novel CAMs by presenting coiled-coil peptides on the cell surface. helixCAMs were able to create specific cell-cell interactions and direct patterned aggregate formation in bacteria and human cells. We built rationally designed helixCAM libraries and discovered novel high-performance helixCAM pairs. High-affinity helixCAMs were then used for multicellular engineering applications, such as spherical layering, adherent cell targeting, and surface patterning. The helixCAMs are an expandable platform for directing complex multicellular assemblies, and we foresee its utility in tissue engineering, immunology, and developmental biology.
Whole-Genome Profiling of Ethyl Methanesulfonate Mutagenesis in Tomato
Authors: Gupta Prateek , Dholaniya Pankaj Singh , Princy Kunnappady , Madhavan Athira Sethu , Sreelakshmi Yellamaraju , Sharma Rameshwar 
Published: April 2022

AbstractThe induced mutations accelerate crop improvement by providing novel disease resistance and yield alleles. However, the alleles with no perceptible phenotype but having an altered function remain hidden in mutagenized plants. The whole-genome sequencing (WGS) of mutagenized individuals uncovers the complete spectrum of mutations in the genome. We sequenced 132 doubly ethyl methanesulfonate (EMS)-mutagenized lines of tomato and detected ca. 41 million SNPs and 5.5 million short-INDELs. We found a very high average density of mutations 1/3.05 Kb compared to other species. About 97% of the genome had mutations, including the genes, promoters, UTRs, and introns. More than 1/3rd of genes in the mutagenized population had one or more deleterious mutations predicted by SIFT. Nearly 1/4th of deleterious genes mapped on tomato metabolic pathways modulating multiple pathway steps. Contrary to the reported GC>AT transition bias for EMS, we found EMS also produced nearly equal AT>GC transitions. Comparing mutation frequency among synonymous codons revealed that the most preferred codon is least mutagenic towards EMS. The reduction in carotenoids in ζ-carotene isomerase mutant fruits and chloroplasts relocation loss in phototropin1 mutant validated the mutation discovery pipeline. Our study makes a large repertoire of mutations accessible to genetic studies and the breeding of tomato.
Using evolutionary game theory to study construction safety supervisory mechanism in China
Authors: Jiang Xiaoyan , Sun Haoyu , Lu Kun , Lyu Sainan , Skitmore Martin 
Published: April 2022

PurposeIn China, external supervision on construction safety mainly comes from the government and supervision engineers (SEs). However, the construction safety supervisory mechanism (CSSM) contains some dilemmas affecting the improvement of safety performance, such as the declining impact of SEs, the increasing rent-seeking behaviors of contractor and excessive government interference. This study aims to depict and analyze the CSSM in China from an evolutionary game view. The objectives are to understand the supervision strategy and evolutionary behaviors of different stakeholders, propose suggestions for improving safety performance and help the key safety supervision stakeholders, especially the government, formulate a suitable safety supervision strategy.Design/methodology/approachThis research uses tripartite dynamic evolutionary game theory to study the CSSM in China and solve the stable equilibrium solution using system dynamics.FindingsThis study has revealed the game relationship of construction safety supervision mechanisms in China and solved the stable equilibrium solution. The results prove that a supervision engineer (SE) plays a crucial role in the CSSM, and “supervision engineer useless” is an unreasonable assertion. For government supervision agency (GSA), excessive inspection and free-market regulation are neither wise strategies. GSA can reduce the inspection frequency when general contractors (GCs) input high safety investments and SEs implement responsible supervision. But keeping proper government supervision to avoid GC's unlawful behaviors and SE's rent-seeking is indispensable. In addition, excessive governmental supervision will weaken SE's role, so the government should transfer some supervision powers to SE.Originality/valueThis study focuses on the dynamic evolution process between GSA, GC and SE. This method is different from most research that neglected the dynamic characteristic of system and game solution stability. The research methods not only contribute to construction safety supervision policy-making in China but also help to improve supervision efficiency in other countries and other fields.
Putting passion to work: passionate labour in the fashion blogosphere
Authors: McFarlane Ashleigh , Hamilton Kathy , Hewer Paul 
Published: April 2022

PurposeThis study aims to explore passionate labour in the fashion blogosphere and addresses two research questions: How does passion animate passionate labour? How does the emotion of passions and the discipline of labour fuse within passionate labour?Design/methodology/approachThis study presents a three-year netnographic fieldwork of replikate fashion blogger-preneurs. Data are based on in-depth interviews, blogs, social media posts and informed by the relationships developed across these platforms.FindingsThroughout the findings, this study unpacks the “little passions” that animate the passionate labour of blogger-preneurs. Passions include: passion for performing the royal lifestyle, the mobilisation of passion within strategic sociality and transformation and self-renewal through blogging. Lastly, the cycle of passion illustrates how passions can be recycled into new passionate projects.Research limitations/implicationsThis study offers insight on how passionate labour requires the negotiation and mobilisation of emotion alongside a calculated understanding of market logics.Practical implicationsThis study raises implications for aspiring blogger-preneurs, luxury brand managers and organisations beyond the blogging context.Originality/valueThe contribution of this study lies in the cultural understanding of passion as a form of labour where passion has become a way of life. The theorisation of passionate labour contributes to existing research in three ways. First, this study identifies social mimesis as a driver of passionate labour and its links to class distinction. Second, it offers insight on how passionate labour requires the negotiation and mobilisation of emotion alongside a calculated understanding of market logics. Third, it advances critical debate around exploitation and inequality within digital labour by demonstrating how passion is unequally distributed.