bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined anaerobic digestion of chicken manure and corn straw: study on methanogenic potential and microbial diversity
Authors: Feng Lei , Lin Xiaoyu , Li Xu 
Published: November 2022

Abstract
Purpose
To explore the methane production potential and microbial community changes of combined anaerobic digestion of chicken manure and corn straw. Increase methane production, reduce the environmental pollution caused by the burning of livestock manure and straw, and provide some theoretical references for the construction and operation of actual biogas projects.

Methods
Different proportions (3%, 5%, 10%) of corn straw were added to the anaerobic digestion systems of chicken manure in order to improve the C/N ratio and to evaluate the feasibility and potential synergistic effect on the co-digestion. The key point was to use 16S rDNA sequencing to analyze the relationship between the microbial diversity and the hydrolase activity during the anaerobic digestion.

Result
The results showed that the volumetric gas production of methane in the 3% straw addition group was 227.66 ml/gVS, which was 18% higher than the cumulative methane production in the pure chicken manure experimental group. However, with the increase of straw concentration, methane production and the utilization rate of the raw materials continued to decrease. The change in activity of each hydrolase was in agreement with changes in hydrolytic acidifying bacteria, and the activity of the main hydrolase also increased with the addition of straw; the correlation coefficient was 0.9943. Sequencing results showed that the dominant strains of methanogenic archaea were Methanosarcina, Methanosaeta, Methanobacterium, and Methanospirillum. Mainly for hydrogen-eating, acetic acid-eating methanogens, its role is to use H2, methanol and acetic acid, and other substances to metabolize methane, and convert it into CH4 and CO2.

Conclusion
The addition of a small amount of straw enhanced the production capacity of hydrogen-nutritive methane to some extent, and the species richness and evenness were also improved, reducing the pollution caused by livestock manure to the environment while controlling the pollution caused by straw burning.

Graphical Abstract
Methotrexate-induced acute cardiotoxicity requiring veno-arterial extracorporeal membrane oxygenation support: a case report
Authors: Shah Sareena , Haeger-Overstreet Kristen , Flynn Brigid 
Published: November 2022

AbstractBackgroundMethotrexate is an antifolate antimetabolite that inhibits the activity of dihydrofolate reductase by acting as a false substrate, which leads to defects of DNA synthesis, specifically the inhibition of purine and pyrimidine synthesis. Thus, methotrexate is a powerful agent for treating autoimmune diseases and cancer. In general, methotrexate is thought to be cardioprotective and reports of methotrexate-induced cardiomyopathy are rare. We present a case of methotrexate-induced severe cardiotoxicity diagnosed by exclusion of all other potential causes.Case presentationThe patient was a 54-year-old Caucasian man presenting to an outside hospital with a chief complaint of abdominal pain and bloating who reported taking methotrexate up to 20 mg per week for systemic sclerosis. After a transthoracic echocardiogram found a left ventricular ejection fraction of 10% and coronary catheterization demonstrated no significant disease, he was transferred to our hospital for advanced heart failure therapies. His condition deteriorated, and he was eventually placed on veno-arterial extracorporeal membrane oxygenation. Owing to a lack of an identifiable etiology of cardiac failure, toxicology consultation recommended 24 hours of intravenous leucovorin therapy to overcome any residual and potentially cardiotoxic methotrexate still in his system. Over the next 5 days, his cardiac function improved daily, such that on day 5 of extracorporeal membrane oxygenation, he had a left ventricular ejection fraction of 40% and was able to be decannulated. Two days later, his ejection fraction improved to 60% and normal right ventricular function. Initially, his renal function improved while on extracorporeal membrane oxygenation, but over the next week deteriorated such that he required intermittent hemodialysis until hospital discharge.ConclusionsAfter a process of elimination, the most likely cause of this patient’s acute decline and rapid recovery of bi-ventricular function was methotrexate toxicity. Leucovorin may have aided the reversal of methotrexate toxicity.
The revised recommendation for administering vitamin C in septic patients: the Japanese Clinical Practice Guidelines for Management of Sepsis and Septic Shock 2020
Authors:   Guideline committee of The Japanese Clinical Practice Guidelines for Management of Sepsis and Septic Shock 2020,   Japanese Society of Intensive Care Medicine, Egi Moritoki ,   Japanese Association for Acute Medicine, Ogura Hiroshi 
Published: November 2022

AbstractGiven the available clinical evidence through the literature search when the Japanese Clinical Practice Guidelines for Management of Sepsis and Septic Shock 2020 was being created, we suggested administering vitamin C to such patients. Recently, several randomized control trials have been published, some of which suggested the harmful effect of vitamin C in terms of mortality or persistent organ dysfunction. Therefore, we performed updated systematic reviews and meta-analyses. Accordingly, we revised our recommendation as “We suggest against administering vitamin C to septic patients (GRADE 2D: certainty of evidence = “very low”).”
Resolving the conflicts around Par2 opposing roles in regeneration by comparing immune-mediated and toxic-induced injuries
Authors: Reches Gal , Blondheim Shraga Netta R. , Carrette Florent , Malka Assaf , Saleev Natalia , Gubbay Yehuda , Ertracht Offir , Haviv Izhak , Bradley Linda M. , Levine Fred , Piran Ron 
Published: November 2022

Abstract
Background
Different factors may lead to hepatitis. Among which are liver inflammation and poisoning. We chose two hepatitis models, typical for these two underlying causes. Thus, we aimed to characterize the role of protease-activated receptor 2 (Par2) in liver regeneration and inflammation to reconcile Par2 conflicting role in many damage models, which sometimes aggravates the induced damage and sometimes alleviates it.

Methods
WT and knockout (Par2KO) mice were injected with concanavalin A (ConA) to induce immune-mediated hepatitis or with carbon tetrachloride (CCl4) to elicit direct hepatic damage. To distinguish the immune component from the liver regenerative response, we conducted bone marrow (BM) replacements of WT and Par2KO mice and repeated the damage models.

Results
ConA injection caused limited damage in Par2KO mice livers, while in the WT mice severe damage followed by leukocyte infiltration was evident. Reciprocal BM replacement of WT and Par2KO showed that WT BM-reconstituted Par2KO mice displayed marked liver damage, while in Par2KO BM-reconstituted WT mice, the tissue was generally protected.
In the CCl4 direct damage model, hepatocytes regenerated in WT mice, whereas Par2KO mice failed to recover. Reciprocal BM replacement did not show significant differences in hepatic regeneration. In Par2KO mice, hepatitis was more apparent, while WT recovered regardless of the BM origin.

Conclusions
We conclude that Par2 activation in the immune system aggravates hepatitis and that Par2 activation in the damaged tissue promotes liver regeneration. When we incorporate this finding and revisit the literature reports, we reconciled the conflicts surrounding Par2’s role in injury, recovery, and inflammation.
Association of cellular immunity with severity of COVID-19 from the perspective of antigen-specific memory T cell responses and cross-reactivity
Authors: Fujii Shin-ichiro , Yamasaki Satoru , Iyoda Tomonori , Shimizu Kanako 
Published: November 2022

AbstractCoronaviruses regularly cause outbreaks of zoonotic diseases characterized by severe pneumonia. The new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused the global pandemic disease COVID-19 that began at the end of 2019 and spread rapidly owing to its infectious nature and rapidly progressing pneumonia. Although the infectivity of SARS-CoV-2 is high, indicated by the worldwide spread of the disease in a very short period, many individuals displayed only subclinical infection, and some of them transmitted the disease to individuals who then developed a severe symptomatic infection. Furthermore, there are differences in the severity of infection across countries, which can be attributed to factors such as the emergence of viral mutations in a short period of time as well as to the immune responses to viral factors. Anti-viral immunity generally consists of neutralizing antibodies that block viral infection and cytotoxic CD8+ T cells that eliminate the virus-infected cells. There is compelling evidence for the role of neutralizing antibodies in protective immunity in SARS-CoV-2 infection. However, the role of CD4+ and CD8+ T cells after the viral entry is complex and warrants a comprehensive discussion. Here, we discuss the protection afforded by cellular immunity against initial infection and development of severe disease. The initial failure of cellular immunity to control the infection worsens the clinical outcomes and functional profiles that inflict tissue damage without effectively eliminating viral reservoirs, while robust T cell responses are associated with mild outcomes. We also discuss persistent long-lasting memory T cell-mediated protection after infection or vaccination, which is rather complicated as it may involve SARS-CoV-2-specific cytotoxic T lymphocytes or cross-reactivity with previously infected seasonal coronaviruses, which are largely related to HLA genotypes. In addition, cross-reactivity with mutant strains is also discussed. Lastly, we discuss appropriate measures to be taken against the disease for immunocompromised patients. In conclusion, we provide evidence and discuss the causal relationship between natural infection- or vaccine-mediated memory T cell immunity and severity of COVID-19. This review is expected to provide a basis to develop strategies for the next generation of T cell-focused vaccines and aid in ending the current pandemic.
Impact of COVID-19 on endoscopic follow-up of gastroesophageal varices
Authors: Elsayed Amr M. , Fouad Yasser M. , Hassan Hatem A. , Hassanin Taha M. , Abbas Abbas M. , Hassnine Alshymaa A. 
Published: November 2022

Abstract
Background
Portal hypertension is considered as a major complication of liver cirrhosis. Endoscopy plays a main role in managing of gastrointestinal complications of portal hypertension. Endoscopists are at increased risk for COVID-19 infection because upper gastrointestinal (GI) endoscopy is a high-risk aerosol-generating procedure and may be a potential route for COVID-19.

Objectives
To compare the outcome between cirrhotic patients who underwent classic regular endoscopic variceal ligation after primary bleeding episode every 2–4 weeks, and those presented during the era of COVID-19 and their follow-up were postponed 2 months later.

Methods
This retrospective study included cross-matched 238 cirrhotic patients with portal hypertension presented with upper GI bleeding, 112 cirrhotic patients presented during the era of COVID19 (group A) underwent endoscopic variceal ligation, another session after 2 weeks and their subsequent follow-up was postponed 2 months later, and 126 cirrhotic patients as control (group B) underwent regular endoscopic variceal band ligation after primary bleeding episode every 2–4 weeks.

Results
Eradication of varices was achieved in 32% of cases in group A, and 46% in group was not any statistically significant (p > 0.05); also, there was no any statistical significant difference between both groups regarding occurrence of rebleeding, post endoscopic symptoms, and mortality rate (p > 0.05).

Conclusion
Band ligation and injection of esophageal and gastric vary every 2 months were as effective and safe as doing it every 2 to 4 weeks after primary bleeding episode for further studies and validation.
Preferred technique of creating pneumoperitoneum for laparoscopy by pediatric surgeons
Authors: Shah Amar A. , Shah Anirudh V. 
Published: November 2022

Abstract
Background
Minimally invasive pediatric surgery has gained popularity over open conventional surgery as it offers benefits to both patients and health care practitioners. Creation of pneumoperitoneum is a must for any laparoscopic procedure. Different pediatric surgeons have their individual preferences regarding the technique to create pneumoperitoneum prior to laparoscopy. The aim of this study was to evaluate the preference of technique for the creation of pneumoperitoneum in pediatric laparoscopy among Indian Pediatric Surgeons. The study was designed by communication with members of the Indian Association of Pediatric Surgeons using a predefined questionnaire. 180 pediatric surgeons responded to the survey. The respondents included pediatric surgeons in institutional and private practice. The reasons behind their preference were enumerated and evaluated.

Results
Seventy-one percent of pediatric surgeons preferred the primary open technique for the creation of pneumoperitoneum. Seventeen percent exclusively used Veress needle whereas 12% were using both techniques varying from patient to patient.

Conclusion
Creation of pneumoperitoneum remains an important safety issue for all pediatric surgeons. Pediatric Surgeons in India prefer using the primary open technique for the creation of pneumoperitoneum for laparoscopic surgery.
The PRINCIPAL Network: A Model to Optimize Infection Care and Prevention in Pediatric Oncology in the Latin American Region
Authors: Caniza Miguela A. , Homsi Maysam R. , Vázquez Milka , Gutierrez Ivan F. , Kopsidas Ioannis , Mukkada Sheena , Friedrich Paola , Metzger Monika 
Published: November 2022

PURPOSE Children with cancer are at high risk for poor outcomes, and health care providers are often unfamiliar with best practices in infection care and prevention (IC&P) in this small and fragile population. Graduates of training courses in IC&P in immunocompromised hosts identified a need for a community that would enable members to share health care experiences, provide resources for continuing medical education, and foster collaborative research and quality improvement opportunities. We developed a Latin American network, Prevencionistas e Infectólogos para Cáncer Pediátrico en América Latina, to grow and sustain the expertise of the clinical workforce in IC&P. Here, we describe the network, how we built it, and its early outcomes. METHODS We began by codesigning the mission, vision, objectives, and values. We then established the structure for leadership and network management to provide a functional uniformity and sustainability. Virtual meetings with network members and strategic in-person gatherings optimized the use of the time and resources of the network. RESULTS The network has seen good participation by members and candidates for membership, who have provided feedback on case-based learning. Members have attended training sessions on quality improvement, research in human subjects, and IC&P in pediatric oncology at national and regional meetings and workshops. Network members have presented their work at regional and global meetings, and publications are beginning to emerge from this community. A direct effect of the Prevencionistas e Infectólogos para Cáncer Pediátrico en América Latina network has been the creation of a similar network for the Asia Pacific region, and a third network is being planned. CONCLUSION We have demonstrated the power of a discipline-specific network structure to facilitate sharing of evidence-based information that enhances the quality-of-care delivery in pediatric oncology.
Comparison of PIK3CA Mutation Prevalence in Breast Cancer Across Predicted Ancestry Populations
Authors: Chen Jessica W. , Murugesan Karthikeyan , Newberg Justin Y. , Sokol Ethan S. , Savage Heidi M. , Stout Thomas J. , Maund Sophia L. , Hutchinson Katherine E. 
Published: November 2022

PURPOSE Understanding the differences in biomarker prevalence that may exist among diverse populations is invaluable to accurately forecast biomarker-driven clinical trial enrollment metrics and to advance inclusive research and health equity. This study evaluated the frequency and types of PIK3CA mutations ( PIK3CAmut) detected in predicted genetic ancestry subgroups across breast cancer (BC) subtypes. METHODS Analyses were conducted using real-world genomic data from adult patients with BC treated in an academic or community setting in the United States and whose tumor tissue was submitted for comprehensive genomic profiling. RESULTS Of 36,151 patients with BC (median age, 58 years; 99% female), the breakdown by predicted genetic ancestry was 75% European, 14% African, 6% Central/South American, 3% East Asian, and 1% South Asian. We demonstrated that patients of African ancestry are less likely to have tumors that harbor PIK3CAmut compared with patients of European ancestry with estrogen receptor–positive/human epidermal growth factor receptor 2–negative (ER+/HER2–) BC (37% [949/2,593] v 44% [7,706/17,637]; q = 4.39E-11) and triple-negative breast cancer (8% [179/2,199] v 14% [991/7,072]; q = 6.07E-13). Moreover, we found that PIK3CAmut were predominantly composed of hotspot mutations, of which mutations at H1047 were the most prevalent across BC subtypes (35%-41% ER+/HER2– BC; 43%-61% HER2+ BC; 40%-59% triple-negative breast cancer). CONCLUSION This analysis established that tumor PIK3CAmut prevalence can differ among predicted genetic ancestries across BC subtypes on the basis of the largest comprehensive genomic profiling data set of patients with cancer treated in the United States. This study highlights the need for equitable representation in research studies, which is imperative to ensuring better health outcomes for all.
Proceedings From the ASCO/College of American Pathologists Immune Checkpoint Inhibitor Predictive Biomarker Summit
Authors: Hayes Daniel F. , Herbst Roy S. , Myles Jonathan L. , Topalian Suzanne L. , Yohe Sophia L. , Aronson Naomi , Bellizzi Andrew M. , Basu Roy Upal , Bradshaw Georganne , Edwards Robin H. , El-Gabry Ehab A. , Elvin Julia , Gajewski Thomas F. , McShane Lisa M. , Oberley Matthew , Philip Reena , Rimm David L. , Rosenbaum Jason N. , Rubin Eric H. , Schlager Lisa , Sherwood Shimere W. , Stewart Mark , Taube Janis M. , Thurin Magdalena , Vasalos Patricia , Laser Jordan 
Published: November 2022

PURPOSEImmune checkpoint inhibition (ICI) therapy represents one of the great advances in the field of oncology, highlighted by the Nobel Prize in 2018. Multiple predictive biomarkers for ICI benefit have been proposed. These include assessment of programmed death ligand-1 expression by immunohistochemistry, and determination of mutational genotype (microsatellite instability or mismatch repair deficiency or tumor mutational burden) as a reflection of neoantigen expression. However, deployment of these assays has been challenging for oncologists and pathologists alike.METHODSTo address these issues, ASCO and the College of American Pathologists convened a virtual Predictive Factor Summit from September 14 to 15, 2021. Representatives from the academic community, US Food and Drug Administration, Centers for Medicare and Medicaid Services, National Institutes of Health, health insurance organizations, pharmaceutical companies, in vitro diagnostics manufacturers, and patient advocate organizations presented state-of-the-art predictive factors for ICI, associated problems, and possible solutions.RESULTSThe Summit provided an overview of the challenges and opportunities for improvement in assay execution, interpretation, and clinical applications of programmed death ligand-1, microsatellite instability-high or mismatch repair deficient, and tumor mutational burden-high for ICI therapies, as well as issues related to regulation, reimbursement, and next-generation ICI biomarker development.CONCLUSIONThe Summit concluded with a plan to generate a joint ASCO/College of American Pathologists strategy for consideration of future research in each of these areas to improve tumor biomarker tests for ICI therapy.
Nonhuman Governance
Authors: Mehtta Megnaa 
Published: November 2022

Abstract
The current ecological crisis and its accompanying environmental consciousness has prodded many to reject Western dualism and instead embrace animism. Taking the Sundarbans forests of India as a starting point, the author shows how several animated, nonhuman agents of the region guide both resource use and social relationships through a set of rules known as the “rules of the jungle.” The source of these rules are deities, demons, and spirits—that is, “cosmic polities”—that undeniably govern life in the Sundarbans and across the landscape of South Asia. Mehtta shows how such nonhuman forms of governance and animistic ontologies can act as a source not only of care and an ecological consciousness but also are capable of exclusion and discrimination. Consequently, the South Asian context provides an important cautionary tale about the blind embrace of animism as the sole savior of our ecological crisis by revealing a spectrum of violence within certain strands of animistic ontologies. Simultaneously the author shows how Western repertoires of thought reveal framing devices that transcend dualism and may be read as the precursors of contemporary environmental consciousness. This article ultimately proposes the importance of acknowledging a bricolage of ontologies and realities without entrenching them in a particular identity of caste, tribe, or “indigeneity” or in being of “the West” or of “the rest of the world.”
Birha
Authors: Judge Rajbir Singh 
Published: November 2022

AbstractFocusing on early twentieth-century Punjab, this article considers how situating the region into historical context circumscribes the literary by tying it to place, thereby creating a seamless economy of exchange. In contrast, noting the refusal of literary and artistic output to be adjudicated into context, this article asks, Is it possible to consider the encounters within the Punjabi literary and artistic scene through a dislocation rather than a circuitous exchange within a singular Punjab? The author ponders this question by considering how analyses centered on exchange are unavoidable when situated within historicity—analyses that emerged in the colonial period as a central way to understand Sikh literary production. Such a grasp on Punjab, the Sikh tradition, and historicity, however, is loosened when we consider the nonhuman. The nonhuman, in other words, challenges the overt focus on history, conquest, and vision that undergirds our understanding of the Punjabi literary scene by functioning as an impediment to mediation, translation, and recognition. The focus on the nonhuman is not to offer a more robust or precise recognition to Punjab but to disarticulate the very contours of recognition through a focus on the eye.