bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasma Circulating Tumor Epstein–Barr Virus for the Surveillance of Cancer Progression in Bone-Only Metastatic Nasopharyngeal Carcinoma
Authors: Liu Guo-Ying , Xia Wei-Xiong , Bi Zhuo-Fei , Lu Nian , Li Wang-Zhong , Bei Wei-Xin , Liang Hu , Xie Jun-Zhi , Liu Yi-Min , Yao He-Rui , Xiang Yan-Qun 
Published: June 2022

BackgroundPlasma Epstein–Barr virus DNA (EBV-DNA) is a sensitive and specific biomarker for nasopharyngeal carcinoma (NPC). We investigated whether longitudinal monitoring of EBV-DNA could accurately detect clinical disease progression in NPC patients with bone-only metastases.MethodsIn this retrospective study, a total of 105 patients with bone-only metastatic NPC who were treated with platinum-based first-line chemotherapy were enrolled. Undetectable EBV-DNA after first-line chemotherapy was defined as a biochemical complete response (BCR). The correlation of the EBV-DNA dynamic status with overall survival (OS) and progression-free survival (PFS) was determined by Cox regression. The correlation between non-normalized EBV-DNA period and PFS period was determined.ResultsAfter a median follow-up time of 53.4 months [Interquartile range (IQR): 42.8–80.6], 64 patients had disease progression. Thirty-nine of 105 patients (37.1%) had a BCR at all follow-up time points, and none of these 39 patients had disease progression, corresponding to a negative predictive value (NPV) of 100%. Sixty-six patients had a detectable EBV-DNA during surveillance, with 64 diagnosed as disease progression at the last follow-up, for a positive predictive value (PPV) of 97.0%. Actuarial 3-year OS rates were 45.0% for patients with detectable EBV-DNA during posttreatment surveillance and 100% for patients with undetectable EBV-DNA. Lastly, median lead time between non-normalized EBV-DNA and clinically proven progression was 5.87 ± 0.67 months.ConclusionsTaken together, EBV-DNA provided predictive value for the bone-only metastatic NPC patients. The results should be validated in prospective randomized studies.
Construction of ceRNA Networks Associated With CD8 T Cells in Breast Cancer
Authors: Chen Zhilin , Feng Ruifa , Kahlert Ulf Dietrich , Chen Zhitong , Torres-dela Roche Luz Angela , Soliman Amr , Miao Chen , De Wilde Rudy Leon , Shi Wenjie 
Published: June 2022

BackgroundThe infiltration of CD8 T cells is usually linked to a favorable prognosis and may predict the therapeutic response of breast cancer patients to immunotherapy. The purpose of this research is to investigate the competing endogenous RNA (ceRNA) network correlated with the infiltration of CD8 T cells.MethodsBased on expression profiles, CD8 T cell abundances for each breast cancer (BC) patient were inferred using the bioinformatic method by immune markers and expression profiles. We were able to extract the differentially expressed RNAs (DEmRNAs, DEmiRNAs, and DElncRNAs) between low and high CD8 T-cell samples. The ceRNA network was constructed using Cytoscape. Machine learning models were built by lncRNAs to predict CD8 T-cell abundances. The lncRNAs were used to develop a prognostic model that could predict the survival rates of BC patients. The expression of selected lncRNA (XIST) was validated by quantitative real-time PCR (qRT-PCR).ResultsA total of 1,599 DElncRNAs, 89 DEmiRNAs, and 1,794 DEmRNAs between high and low CD8 T-cell groups were obtained. Two ceRNA networks that have positive or negative correlations with CD8 T cells were built. Among the two ceRNA networks, nine lncRNAs (MIR29B2CHG, NEAT1, MALAT1, LINC00943, LINC01146, AC092718.4, AC005332.4, NORAD, and XIST) were selected for model construction. Among six prevalent machine learning models, artificial neural networks performed best, with an area under the curve (AUC) of 0.855. Patients from the high-risk category with BC had a lower survival rate compared to those from the low-risk group. The qRT-PCR results revealed significantly reduced XIST expression in normal breast samples, which was consistent with our integrated analysis.ConclusionThese results potentially provide insights into the ceRNA networks linked with T-cell infiltration and provide accurate models for T-cell prediction.
Molecular Spectrum, Ethnic and Geographical Distribution of Thalassemia in the Southern Area of Hainan, China
Authors: Yu Ying , Lu Chunjiao , Gao Ying , Li Cuiyun , Li Dongxue , Wang Jie , Wei Hui , Lu Zhaohui , You Guoling 
Published: June 2022

BackgroundThalassemia is one of the most common genetic diseases in southern China. Accurate population frequency data regarding the occurrence and distribution of thalassemia are important for designing appropriate prevention strategies for thalassemia. This study aims to reveal the molecular spectrum, ethnic and geographical distribution of thalassemia in the southern area of Hainan Province, China.MethodsA total of 9813 suspected carriers of thalassemia were screened for genetic analysis by using the PCR-reverse dot blot hybridization method targeting three known deletions of α-thalassemias (--SEA, -α3.7, and -α4.2), three nondeletional mutations of α-thalassaemias (αCS, αQS, and αWS) and the 17 most common mutations of β-thalassaemias in the Chinese population.ResultsApproximately 6,924 subjects were genetically diagnosed as thalassemia carriers or patients, including 5812 cases of α-thalassemia (83.9%), 369 cases of β-thalassemia (5.3%), and 743 cases of α-composite β-thalassemia (10.7%). A total of 21 distinct genotypes were identified among the 5,812 α-thalassemia carriers, -α4.2/αα, -α3.7/αα, and -α3.7/-α4.2 were the most common α-thalassemia genotypes. The most frequent β-thalassemia genotype was βCD41−42/βN, with a notable proportion of 69.6%, followed by the β−28M/βN, βIVS−II−654/βN, βCD71−72/βN, βE/βN, and βCD17/βN genotypes. In addition, 37 genotypes were detected among the 743 cases of both α- and β-thalassemia mutations. The α-thalassemia genotypes were most commonly found in the Li people, who accounted for 73.5% of α-thalassemia carriers. The β-thalassemia genotypes were most commonly identified in the Han people, who accounted for 59.4% of β-thalassemia carriers. Among the subjects carrying both α- and β-thalassemia variations, only three ethnic minorities were identified, including the Li, Han, and Miao people, accounting for 82.0, 17.4, and 0.7%, respectively.ConclusionsOur study indicates that there is high genetic heterogeneity, geographical and ethnic differences in thalassemia in populations in the southern area of Hainan Province. These findings will be helpful in guiding genetic counseling and prenatal diagnosis of thalassemia in Hainan Province.
Differences in Multicomponent Pharmacokinetics, Tissue Distribution, and Excretion of Tripterygium Glycosides Tablets in Normal and Adriamycin–Induced Nephrotic Syndrome Rat Models and Correlations With Efficacy and Hepatotoxicity
Authors: Wu Wei , Cheng Rui , Boucetta Hamza , Xu Lei , Pan Jing–ru , Song Min , Lu Yu–ting , Hang Tai–jun 
Published: June 2022

Tripterygium glycosides tablets (TGT) are widely used for treating nephrotic syndrome (NS), but hepatotoxicity is frequently reported. The presence of underlying disease(s) can alter the disposition of drugs and affect their efficacy and toxicity. However, no studies have reported the impact of NS on the ADME profiles of TGT or its subsequent impact on the efficacy and toxicity. Thus, the efficacy and hepatotoxicity of TGT were evaluated in normal and NS rats after oral administration of TGT (10 mg/kg/day) for 4 weeks. The corresponding ADME profiles of the six key TGT components (triptolide (TPL), wilforlide A (WA), wilforgine (WFG), wilfortrine (WFT), wilfordine (WFD), and wilforine (WFR)) were also measured and compared in normal and NS rats after a single oral gavage of 10 mg/kg TGT. Canonical correlation analysis (CCA) of the severity of NS and the in vivo exposure of the six key TGT components was performed to screen the anti–NS and hepatotoxic material bases of TGT. Finally, the efficacy and hepatotoxicity of the target compounds were evaluated in vitro. The results showed that TGT decreased the NS symptoms in rats, but caused worse hepatotoxicity under the NS state. Significant differences in the ADME profiles of the six key TGT components between the normal and NS rats were as follows: higher plasma and tissue exposure, lower urinary and biliary excretion, and higher fecal excretion for NS rats. Based on CCA and in vitro verification, TPL, WA, WFG, WFT, WFD, and WFR were identified as the anti–NS material bases of TGT, whereas TPL, WFG, WFT, and WFD were recognized as the hepatotoxic material bases. In conclusion, NS significantly altered the ADME profiles of the six key TGT components detected in rats, which were related to the anti–NS and hepatotoxic effects of TGT. These results are useful for the rational clinical applications of TGT.
Perceived Peer Relationships in Adolescence and Loneliness in Emerging Adulthood and Workplace Contexts
Authors: Chiao Chi , Lin Kuan-Chen , Chyu Laura 
Published: June 2022

BackgroundA common life-course hypothesis is that negative early-life experiences contribute to poor health in later-life. However, little is known about perceived peer relationships during adolescence and the feeling of loneliness in emerging adulthood. This study explores the perception of adolescent peer relationships in a school context and its association with loneliness in adulthood and in workplace contexts.MethodsThis study used data from a cohort sample of 2,520 adolescents from the Taiwan Youth Project (N = 2,520), consisting of eleven waves of data collected from 2000 to 2017. Major measures included the Loneliness Scale (6-item de Jong Gierveld short scale) and perceived peer relationships (classroom cohesion and perceived popularity among classmates) in middle school. Multivariate multinomial logistic regressions were used to estimate the associations of perceived peer relationships during adolescence and workplace characteristics with loneliness in adulthood.ResultsPositive perceived peer relationships in adolescence were significantly related to decreased risk of serious social loneliness [Relative risk ratios (RRR) 0.70, 95% CI: 0.58–0.85] and severe social/emotional loneliness (RRR = 0.76, 95% CI: 0.63–0.91) in adulthood. Workplace satisfaction was a protective factor of severe social/emotional loneliness in employed adults.ConclusionAdolescents who perceived peer relationships in middle school as positive were less likely to report social and emotional loneliness during adulthood. Satisfaction in the workplace characteristics was also associated with lower risk of loneliness in adulthood. Theoretical and policy implications are discussed.
Turnover Intention and Its Associated Factors Among Psychiatrists in 41 Tertiary Hospitals in China During the COVID-19 Pandemic
Authors: Yang Yating , Zhang Ling , Li Mengdie , Wu Xiaodong , Xia Lei , Liu Daphne Y. , Liu Tingfang , Liu Yuanli , Jiang Feng , Tang Yi-lang , Liu Huanzhong , Kalow Nadine J. 
Published: June 2022

BackgroundTurnover intention, an employee’s intention to voluntarily leave their jobs, affects workforce sustainability. However, scarce data are available about turnover intention and its associated factors among psychiatrists in China, especially during the COVID-19 pandemic. The current research was designed to address this gap.MethodsAn anonymous, nationwide online survey was disseminated to psychiatrists in 41 psychiatric hospitals in China. We collected demographic data, job-related information (duration of employment, history of participation in the frontline work against COVID-19, job satisfaction), and participants’ turnover intention in the next 12 months.ResultsIn total, 3,973 psychiatrists completed the survey. The sample was predominantly female (58.8%) and married (79.5%), and nearly three-fourths (73.5%) had children. More than one-third (35.6%) had a master’s or doctoral degree in addition to their medical degree. The overall level of job satisfaction was moderate. The rate of turnover intention was 22.0% and was comparable in males and females (22.9% in males and 21.3% in females, respectively). Psychiatrists who had participated in the frontline work of COVID-19 were more likely to report an intention to leave their current job. Multiple regression analyses suggested that turnover intention was significantly associated with having insomnia, longer working hours, and more working days per week.ConclusionDuring the pandemic period, approximately one-fifth of psychiatrists in China reported turnover intention. Factors associated with turnover intention included high job-related burdens, low job satisfaction, participation in the frontline work against COVID-19, and insomnia. To improve psychiatric workforce sustainability, policymakers and hospital administrators need to be aware of this potential challenge and address the concerns of psychiatrists in China.
RETRACTED: Association of Self-Leadership With Acute Stress Responses and Acute Stress Disorders in Chinese Medics During the COVID-19 Pandemic: A Cross-Sectional Study
Authors: Ji Rongjian , Zhang Lan , Ji Yanbo , Li Guangzhao , Wang Renxiu , Xu Cuiping 
Published: June 2022

BackgroundThe outbreak of the highly infectious coronavirus disease 2019 (COVID-19) renders a huge physical and psychological risk to the public, especially to the medics. Additionally, self-leadership has proven to improve self-efficacy and mediate tension, such as nervousness and depression. Therefore, a cross-sectional survey was conducted to explore the association of self-leadership with acute stress responses (ASRs) and acute stress disorders (ASDs) in medics during the COVID-19 epidemic.MethodsSelf-reported online questionnaires were administered, and 627 participants were finally included. The data were analyzed using the univariate analysis and the logistical regression model to identify whether self-leadership and sociodemographic and epidemic characteristics were associated with mental health, including ASRs and ASDs.ResultsInitially, 790 medics responded. Of these, 627 remained after excluding for invalid questionnaires and those with a substantial amount of missing data. Therefore, the participation validity rate was 79.37%. Frontline medical staff (β = 0.338; p &lt; 0.001), possibility of infection among people around the medic being mild (β = 0.141; p &lt; 0.001), subjective estimation of epidemic duration being 3–6 months (β = 0.074; p &lt; 0.05), self-sets (β = −0.022; p &lt; 0.001), self-punishment (β = 0.229; p &lt; 0.001), belief hypothesis and evaluation (β = −0.147; p &lt; 0.05), and successful foresight (β = 0.105; p &lt; 0.05) were statistically significant with ASRs. Marital status [adjusted odds ratio (AOR) =1.813; 95% CI (1.141, 2.881); p = 0.012], being a frontline worker [AOR = 25.760; 95% CI (14.220, 46.667); p &lt; 0.001], visiting Hubei in the previous 14 days [AOR = 3.656; 95% CI (1.500, 8.911); p = 0.004], self-punishment [AOR = 1.352; 95% CI (1.180, 1.548); p &lt; 0.001], and self-dialogue [AOR = 1.256; 95% CI (11.063, 1.483); p = 0.007] were the risk factors for ASD. Conversely, having frontline medical staff in one's family [AOR = 0.523; 95% CI (0.297, 0.923); p = 0.025], self-sets [AOR = 0.814; 95% CI (0.715, 0.826); p = 0.002], and belief hypothesis and evaluation [AOR = 0.796; 95% CI (0.672, 0.943); p = 0.038] were the protective factors.ConclusionThe special working environment of the COVID-19 epidemic resulted in ASR and ASD. Notably, findings revealed a positive association between ASR symptoms and frontline medical staff, the subjective estimation of epidemic duration, self-punishment, and successful foresight. Nevertheless, marital status, having visited Hubei in the previous 14 days, and self-dialogue were the risk factors accounting for ASD symptoms. Surprisingly, having frontline medical staff in one's family, self-sets, and belief hypothesis and evaluation had potential benefits for ASD symptoms.
Affective Responses to Both Climbing and Nordic Walking Exercise Are Associated With Intermediate-Term Increases in Physical Activity in Patients With Anxiety and Posttraumatic Stress Disorder - A Randomized Longitudinal Controlled Clinical Pilot Trial
Authors: Bichler Carina S. , Niedermeier Martin , Hüfner Katharina , Gálffy Mátyás , Sperner-Unterweger Barbara , Kopp Martin 
Published: June 2022

BackgroundExercise programs have shown anxiolytic effects in psychiatric patients. Adherence to exercise programs and subsequent long-term lifestyle change is influenced by acute affective responses of the exercise programs. This research aimed to assess acute affective responses of two different exercise modalities compared to a non-exercise control program and its effects on persisting physical activity behavior change.MethodsSixty-six outpatients diagnosed with an anxiety disorder or posttraumatic stress disorder were randomly allocated to one of three groups in a randomized longitudinal controlled clinical pilot trial: climbing (n= 26), nordic walking (n= 19), social contact control (n= 21). Affective responses were assessed pre, during, and post activity. General physical activity behavior was recorded prior to participation in the program, post program, and at follow-ups three and six months after the program.ResultsMultilevel modeling analyzes of 1,066 individual data points revealed increases in affective valence in the exercise sessions compared to the social contact sessions. State anxiety decreased in the climbing group compared to the social contact group. Physical activity behavior was increased immediately following the program as well as at six months follow-up in both exercise groups. A larger increase in affective valence during and after the sessions was associated with higher physical activity post program.ConclusionsClimbing and conventional nordic walking exercise sessions revealed positive affective changes in outpatients indicating therapeutic potential of both modalities for acute emotion regulation. In accordance with theoretical models of human behavior change, it was judged that the experience of a more pleasant affective state following the exercise sessions induced more persisting effects on physical activity behavior after the exercise programs.Trial Registrationhttps://www.clinicaltrials.gov/ct2/show/NCT03758599, identifier: NCT03758599.
Maternal Depression and Associated Factors Among Pregnant Women Attending Ante Natal Care, Southern Ethiopia: Cross-Sectional Study
Authors: Borie Yacob Abraham , Siyoum Melese , Tsega Aklile , Anbese Gemechu 
Published: June 2022

BackgroundPatients with depressed mental disorders may experience a lack of interest or pleasure, a poor mood, feelings of guilt or unworthiness, sleep and appetite disturbances, and easy fatigability. Based on the degree of the condition, depression is classed as mild, moderate, or severe. Maternal depression is the most common psychiatric condition during pregnancy, and its harmful effects have serious ramifications for both the mother and the fetus. Almost one in every four women will experience depression at some point in her life, the majority of which will occur during her childbearing years. Studies reports showed that antenatal depression is a common maternal problem in Ethiopia and as reported antenatal depression ranges in Ethiopia from 19.04 to 29.92%.ObjectiveTo assess the prevalence of maternal depression and associated factors among antenatal care attendants at Wolayta Sodo Teaching and Referral hospital, Southern Ethiopia.MethodsHealth facility based cross-sectional study was conducted at Wolayta Sodo Teaching and Referral Hospital from May 01 to 30, 2018. Data were collected from through face to face interview at exit from antenatal care unit using structured questionnaire and checklist adopted from patient Health Questionnaire (PHQ-9). Data were collected from 309 antenatal attendant mothers using systematic random sampling from each either mother. Binary and multivariable logistic regression model was employed to identify factors associated with maternal depression at P-value &lt;0.05 level of significant.ResultDepression among pregnant mother was found to be 27.2% (95% Cl: 22, 32%). Women's level of education; being elementary school (AOR = 6.35 95%CL (2.32, 17.38), completing high school and above (AOR = 3.35, 95%CL 1.33, 8.47) were associated with maternal depression whereas having good husband support was protective for maternal depression (AOR = 0.4, 95%CL: 0.19, 0.83) and also not using substance during pregnancy period was protective for maternal depression (AOR = 0.39, 95%CI, 0.19, 0.77).ConclusionThe frequency of mother depression in this community was greater than in previous Ethiopian studies reported. Maternal depression was linked to a woman's level of education, husband support, and substance usage. This suggested that health care providers regarding pregnant women should emphasize the importance of husband support, substance usage and women level of education.
Inflammation, Oxidative Stress, and Antioxidant Micronutrients as Mediators of the Relationship Between Sleep, Insulin Sensitivity, and Glycosylated Hemoglobin
Authors: Kanagasabai Thirumagal , Riddell Michael C. , Ardern Chris I. 
Published: June 2022

BackgroundSleep deprivation and poor sleep quality contribute to increases in oxidative stress, antioxidant imbalance, and a pro-inflammatory state which may predispose to a higher risk of diabetes. Our objective was to estimate the contributions of C-reactive protein (CRP), gamma glutamyl transferase (GGT), and micronutrient antioxidants (bilirubin, carotenoids, uric acid, vitamins A, C–E?) to the relationships between sleep-fasting insulin concentration and -glycosylated hemoglobin (HbA1c).MethodsData from the 2005/06 US National Health and Nutritional Examination Survey were used (N = 1,946; 20 y+). Sleep quality and quantity was assessed by the Sleep Disorders Questionnaire, and fasting blood was collected to quantify CRP, GGT, antioxidant micronutrients, insulin concentration, and HbA1c. The bootstrap method was used to estimate the amount of mediation or contribution of these mediators to the sleep–insulin concentration and -HbA1c relationships, which were quantified as large (≥0.25) or moderate (≥0.09).ResultsThe sleep duration–fasting insulin relationship was mediated by GGT, carotenoids, uric acid, and vitamins C and D, whereas CRP and bilirubin were non-significant mediators of a moderate effect size. Similarly, the sleep quality–fasting insulin relationship was mediated by CRP, bilirubin and vitamin C, whereas GGT, carotenoids, uric acid, and vitamin D were non-significant large-to-moderate mediators. To a lesser degree, these micronutrients mediated for the relationship between sleep-HbA1c levels.ConclusionSeveral factors related to inflammation, oxidative stress, and antioxidant status were found to lie on the pathway of the sleep–insulin and –glycemic control relationships. Sleep hygiene, reduced systemic inflammation/oxidative stress, and optimal antioxidants intake are potentially beneficial targets for managing diabetes risk.