bluuebunny/crossref_metadata_2025_split huggingface dataset:
A randomized implementation trial to increase adoption of evidence-informed consent practices
Authors: Solomon Erin D. , Mozersky Jessica , Goodman Melody , Parsons Meredith V. , Baldwin Kari A. , Friedrich Annie B. , Harris Jenine K. , DuBois James M. 
Published: December 2022

Abstract

Introduction:
Several evidence-informed consent practices (ECPs) have been shown to improve informed consent in clinical trials but are not routinely used. These include optimizing consent formatting, using plain language, using validated instruments to assess understanding, and involving legally authorized representatives when appropriate. We hypothesized that participants receiving an implementation science toolkit and a social media push would have increased adoption of ECPs and other outcomes.

Methods:
We conducted a 1-year trial with clinical research professionals in the USA (n = 1284) who have trials open to older adults or focus on Alzheimer’s disease. We randomized participants to receive information on ECPs via receiving a toolkit with a social media push (intervention) or receiving an online learning module (active control). Participants completed a baseline survey and a follow-up survey after 1 year. A subset of participants was interviewed (n = 43).

Results:
Participants who engaged more with the toolkit were more likely to have tried to implement an ECP during the trial than participants less engaged with the toolkit or the active control group. However, there were no significant differences in the adoption of ECPs, intention to adopt, or positive attitudes. Participants reported the toolkit and social media push were satisfactory, and participating increased their awareness of ECPs. However, they reported lacking the time needed to engage with the toolkit more fully.

Conclusions:
Using an implementation science approach to increase the use of ECPs was only modestly successful. Data suggest that having institutional review boards recommend or require ECPs may be an effective way to increase their use.
Bridging the Grade Gap: Reducing Assessment Bias in a Multi-Grader Class
Authors: Kates Sean , Paulsen Tine , Yntiso Sidak , Tucker Joshua A. 
Published: December 2022

AbstractMany large survey courses rely on multiple professors or teaching assistants to judge student responses to open-ended questions. Even following best practices, students with similar levels of conceptual understanding can receive widely varying assessments from different graders. We detail how this can occur and argue that it is an example of differential item functioning (or interpersonal incomparability), where graders interpret the same possible grading range differently. Using both actual assessment data from a large survey course in Comparative Politics and simulation methods, we show that the bias can be corrected by a small number of “bridging” observations across graders. We conclude by offering best practices for fair assessment in large survey courses.
Ultra-processed foods in public health nutrition: the unanswered questions
Authors: Gibney Michael J. 
Published: December 2022

AbstractThere is a growing interest in the study of the degree of food processing and both health and nutritional outcomes. To that end, several definitions of the degree of processing have been proposed. However, when each of these is used on a common database of nutritional, clinical and anthropometric variables, the observed effect of high intakes of highly processed food, varies considerably.. Moreover, assigning a given food by nutritional experts, to its appropriate level of processing, has been shown to be variable. Thus, the subjective definitions of the degree of food processing and the coding of foods according to these classifications is prone to error is prone to error. Another issue that need resolution is the relative importance of the degree of food processing and the formulation of a processed food. Although correlational studies linking processed food and obesity abound, there is a need for more investigative studies.
Immunohistochemical localisation of aquaporin 2 and vasopressin type 2 receptor in the human endolymphatic sac
Authors: Pan X , Huang C , Peng A , Zhang Z 
Published: December 2022

AbstractObjectiveThis study aimed to determine the distribution and subcellular localisation of aquaporin 2 and vasopressin type 2 receptor in the human endolymphatic sac.MethodsTen samples of human endolymphatic sac were collected during acoustic neurinoma removal using the translabyrinthine approach. Immunohistochemistry and immunofluorescence were performed using aquaporin 2 and vasopressin type 2 receptor monoclonal antibodies.ResultsConfocal microscopy demonstrated that vasopressin type 2 receptor labelling was expressed in both the apical and basolateral plasma membranes, and in the cytoplasm of the endolymphatic sac epithelium, whereas aquaporin 2 was strongly expressed at the basolateral site of the endolymphatic sac epithelium, in both the intraosseous and extraosseous parts of the endolymphatic sac.ConclusionBoth aquaporin 2 and vasopressin type 2 receptor were detected in the epithelial cells of the human endolymphatic sac, suggesting that this channel may be involved in inner-ear fluid homeostasis. However, strong basolateral expression of aquaporin 2 in endolymphatic sac epithelium suggested that the function of aquaporin 2 may differ between the endolymphatic sac and kidney.
An outbreak of hepatitis A virus infection in a secondary school in England with no undetected asymptomatic transmission among students
Authors: Wensley A. , Smout E. , Ngui S.-L. , Balogun K. , Blomquist P. , Edelstein M. , Greenwood R. , Coles S. , Ingold K. , Sargeant L. , Gent M. , Padfield S. , Hughes G. J. 
Published: December 2022

Abstract
In June 2019 the Health Protection Team in Yorkshire and Humber, England, was notified of cases of hepatitis A virus (HAV) infection in staff at a secondary school. Investigation revealed that an earlier case worked as a food handler in the school kitchen. Indirect transmission through food from the canteen was considered the most likely route of transmission. Cases were described according to setting of exposure. Oral fluid was obtained from students for serological testing. Environmental investigations were undertaken at settings where food handling was considered a potential transmission risk. Thirty-three confirmed cases were linked to the outbreak. All of those tested (n = 31) shared the same sequence with a HAV IB genotype. The first three cases were a household cluster and included the index case for the school. A further 19 cases (16 students, 3 staff) were associated with the school and consistent with indirect exposure to the food handler. One late onset case could not be ruled out as a secondary case within the school and resulted in vaccination of the school population. Five cases were linked to a bakery where a case from the initial household cluster worked as a food server. No concerns about hygiene standards were noted at either the school or the bakery. Oral fluid samples taken at the time of vaccination from asymptomatic students (n = 219, 11–16 years-old) showed no evidence of recent or current infection. This outbreak included household and foodborne transmission but limited (and possibly zero) person-to-person transmission among secondary school students. Where adequate hygiene exists, secondary transmission within older students may not occur.
Toward Understanding of Long‐Term Nitrogen Transport and Retention Dynamics Across German Catchments
Authors: Nguyen Tam V. , Sarrazin Fanny J. , Ebeling Pia , Musolff Andreas , Fleckenstein Jan H. , Kumar Rohini 
Published: December 2022

AbstractLong‐term nitrogen (N) transport and retention dynamics across catchments are not well understood. Using a process‐based model for 89 German catchments, results across study catchments reveal that most N surplus (during 1950–2014) was removed by denitrification (mean ± standard deviation: 58 ± 15%) while the remaining fraction was mostly stored in the soil (14% ± 11%). The mean groundwater transit times in these catchments varied from 3.2 to 20.3 years. These results indicate that past N inputs could continue to affect surface and groundwater quality in the coming years. We identified four catchment groups with distinct archetypal N transport and retention dynamics, which are linked to the catchments' climate, topographic, and geological conditions. Overall, our results shed light on long‐term N dynamics in German catchments and how they are linked to catchment characteristics, emphasizing the role of long‐term N accumulation and transport for water quality management and evaluation programs.
Management of patients with lower-risk myelodysplastic syndromes
Authors: Brunner Andrew M. , Leitch Heather A. , van de Loosdrecht Arjan A. , Bonadies Nicolas 
Published: December 2022

AbstractMyelodysplastic syndromes (MDS) are a heterogeneous group of hematopoietic stem cell disorders characterized by ineffective hematopoiesis with abnormal blood cell development (dysplasia) leading to cytopenias and an increased risk for progression to acute myeloid leukemia (AML). Patients with MDS can generally be classified as lower- (LR-MDS) or higher-risk (HR-MDS). As treatment goals for patients with LR-MDS and those with HR-MDS differ significantly, appropriate diagnosis, classification, and follow-up are critical for correct disease management. In this review, we focus on the diagnosis, prognosis, and treatment options, as well as the prediction of the disease course and monitoring of treatment response in patients with LR-MDS. We discuss how next-generation sequencing, increasing knowledge on mechanisms of MDS pathogenesis, and novel therapies may change the current treatment landscape in LR-MDS and why structured assessments of responses, toxicities, and patient-reported outcomes should be incorporated into routine clinical practice.
Primary exposure to SARS-CoV-2 variants elicits convergent epitope specificities, immunoglobulin V gene usage and public B cell clones
Authors: Lima Noemia S. , Musayev Maryam , Johnston Timothy S. , Wagner Danielle A. , Henry Amy R. , Wang Lingshu , Yang Eun Sung , Zhang Yi , Birungi Kevina , Black Walker P. , O’Dell Sijy , Schmidt Stephen D. , Moon Damee , Lorang Cynthia G. , Zhao Bingchun , Chen Man , Boswell Kristin L. , Roberts-Torres Jesmine , Davis Rachel L. , Peyton Lowrey , Narpala Sandeep R. , O’Connell Sarah , Serebryannyy Leonid , Wang Jennifer , Schrager Alexander , Talana Chloe Adrienna , Shimberg Geoffrey , Leung Kwanyee , Shi Wei , Khashab Rawan , Biber Asaf , Zilberman Tal , Rhein Joshua , Vetter Sara , Ahmed Afeefa , Novik Laura , Widge Alicia , Gordon Ingelise , Guech Mercy , Teng I-Ting , Phung Emily , Ruckwardt Tracy J. , Pegu Amarendra , Misasi John , Doria-Rose Nicole A. , Gaudinski Martin , Koup Richard A. , Kwong Peter D. , McDermott Adrian B. , Amit Sharon , Schacker Timothy W. , Levy Itzchak , Mascola John R. , Sullivan Nancy J. , Schramm Chaim A. , Douek Daniel C. 
Published: December 2022

AbstractAn important consequence of infection with a SARS-CoV-2 variant is protective humoral immunity against other variants. However, the basis for such cross-protection at the molecular level is incompletely understood. Here, we characterized the repertoire and epitope specificity of antibodies elicited by infection with the Beta, Gamma and WA1 ancestral variants and assessed their cross-reactivity to these and the more recent Delta and Omicron variants. We developed a method to obtain immunoglobulin sequences with concurrent rapid production and functional assessment of monoclonal antibodies from hundreds of single B cells sorted by flow cytometry. Infection with any variant elicited similar cross-binding antibody responses exhibiting a conserved hierarchy of epitope immunodominance. Furthermore, convergent V gene usage and similar public B cell clones were elicited regardless of infecting variant. These convergent responses despite antigenic variation may account for the continued efficacy of vaccines based on a single ancestral variant.
Structure of the Acidobacteria homodimeric reaction center bound with cytochrome c
Authors: Dong Shishang , Huang Guoqiang , Wang Changhui , Wang Jiajia , Sui Sen-Fang , Qin Xiaochun 
Published: December 2022

AbstractPhotosynthesis converts light energy to chemical energy to fuel life on earth. Light energy is harvested by antenna pigments and transferred to reaction centers (RCs) to drive the electron transfer (ET) reactions. Here, we present cryo-electron microscopy (cryo-EM) structures of two forms of the RC from the microaerophilic Chloracidobacterium thermophilum (CabRC): one containing 10 subunits, including two different cytochromes; and the other possessing two additional subunits, PscB and PscZ. The larger form contained 2 Zn-bacteriochlorophylls, 16 bacteriochlorophylls, 10 chlorophylls, 2 lycopenes, 2 hemes, 3 Fe4S4 clusters, 12 lipids, 2 Ca2+ ions and 6 water molecules, revealing a type I RC with an ET chain involving two hemes and a hybrid antenna containing bacteriochlorophylls and chlorophylls. Our results provide a structural basis for understanding the excitation energy and ET within the CabRC and offer evolutionary insights into the origin and adaptation of photosynthetic RCs.
Biosynthesis of mushroom-derived type II ganoderic acids by engineered yeast
Authors: Yuan Wei , Jiang Chenjian , Wang Qin , Fang Yubo , Wang Jin , Wang Meng , Xiao Han 
Published: December 2022

AbstractType II ganoderic acids (GAs) produced by the traditional medicinal mushroom Ganoderma are a group of triterpenoids with superior biological activities. However, challenges in the genetic manipulation of the native producer, low level of accumulation in the farmed mushroom, the vulnerabilities of the farming-based supply chain, and the elusive biosynthetic pathway have hindered the efficient production of type II GAs. Here, we assemble the genome of type II GAs accumulating G. lucidum accession, screen cytochrome P450 enzymes (CYPs) identified from G. lucidum in baker’s yeast, identify key missing CYPs involved in type II GAs biosynthesis, and investigate the catalytic reaction sequence of a promiscuous CYP. Then, we engineer baker’s yeast for bioproduciton of GA-Y (3) and GA-Jb (4) and achieve their production at higher level than those from the farmed mushroom. Our findings facilitate the further deconvolution of the complex GA biosynthetic network and the development of microbial cell factories for producing GAs at commercial scale.
St18 specifies globus pallidus projection neuron identity in MGE lineage
Authors: Nunnelly Luke F. , Campbell Melissa , Lee Dylan I. , Dummer Patrick , Gu Guoqiang , Menon Vilas , Au Edmund 
Published: December 2022

AbstractThe medial ganglionic eminence (MGE) produces both locally-projecting interneurons, which migrate long distances to structures such as the cortex as well as projection neurons that occupy subcortical nuclei. Little is known about what regulates the migratory behavior and axonal projections of these two broad classes of neurons. We find that St18 regulates the migration and morphology of MGE neurons in vitro. Further, genetic loss-of-function of St18 in mice reveals a reduction in projection neurons of the globus pallidus pars externa. St18 functions by influencing cell fate in MGE lineages as we observe a large expansion of nascent cortical interneurons at the expense of putative GPe neurons in St18 null embryos. Downstream of St18, we identified Cbx7, a component of Polycomb repressor complex 1, and find that it is essential for projection neuron-like migration but not morphology. Thus, we identify St18 as a key regulator of projection neuron vs. interneuron identity.
Face pareidolia in male schizophrenia
Authors: Romagnano Valentina , Sokolov Alexander N. , Steinwand Patrick , Fallgatter Andreas J. , Pavlova Marina A. 
Published: December 2022

AbstractFaces are valuable signals for efficient social interaction. Yet, social cognition including the sensitivity to a coarse face scheme may be deviant in schizophrenia (SZ). Tuning to faces in non-face images such as shadows, grilled toasts, or ink blots is termed face pareidolia. This phenomenon is poorly investigated in SZ. Here face tuning was assessed in 44 male participants with SZ and person-by-person matched controls by using recently created Face-n-Thing images (photographs of non-face objects to a varying degree resembling a face). The advantage of these images is that single components do not automatically trigger face processing. Participants were administered a set of images with upright and inverted (180° in the image plane) orientation. In a two-alternative forced-choice paradigm, they had to indicate whether an image resembled a face. The findings showed that: (i) With upright orientation, SZ patients exhibited deficits in face tuning: they provided much fewer face responses than controls. (ii) Inversion generally hindered face pareidolia. However, while in neurotypical males, inversion led to a drastic drop in face impression, in SZ, the impact of orientation was reduced. (iii) Finally, in accord with the signal detection theory analysis, the sensitivity index (d-prime) was lower in SZ, whereas no difference occurred in decision criterion. The outcome suggests altered face pareidolia in SZ is caused by lower face sensitivity rather than by alterations in cognitive bias. Comparison of these findings with earlier evidence confirms that tuning to social signals is lower in SZ, and warrants tailored brain imaging research.
Understanding protective and risk factors affecting adolescents’ well-being during the COVID-19 pandemic
Authors: Lan Min , Pan Qianqian , Tan Cheng Yong , Law Nancy Wai Ying 
Published: December 2022

AbstractThis study investigated the factors affecting adolescents’ well-being during the COVID-19 pandemic from the perspectives of their participation in digital activities, emotional regulation, self-regulated learning, and parental involvement. Using self-reported data from 932 pairs of adolescents and their parents, we performed multiple-group structural equation modeling, which revealed that self-efficacy in online learning during school suspension was a key factor influencing adolescents’ perceived worries after schools resumed. During school suspension, boys’ cognitive-emotional regulation played a protective role in their well-being, helping them to avoid cyberbullying incidents, while girls’ participation in leisure-oriented digital activities compromised their self-efficacy in online learning and led to cyberbullying incidents. Furthermore, improvement in parent–child relationships during school suspension encouraged adolescents to use more positive emotional regulation strategies, enhanced their self-efficacy in online learning, and reduced their leisure-time digital activities. The findings indicate that the effective regulation of adolescents’ online behaviors, emotions, and self-efficacy, especially when combined with an emotionally secure family relationship, can ensure adolescents’ well-being.
A novel capsid protein network allows the characteristic internal membrane structure of Marseilleviridae giant viruses
Authors: Chihara Akane , Burton-Smith Raymond N. , Kajimura Naoko , Mitsuoka Kaoru , Okamoto Kenta , Song Chihong , Murata Kazuyoshi 
Published: December 2022

AbstractMarseilleviridae is a family of giant viruses, showing a characteristic internal membrane with extrusions underneath the icosahedral vertices. However, such large objects, with a maximum diameter of 250 nm are technically difficult to examine at sub-nanometre resolution by cryo-electron microscopy. Here, we tested the utility of 1 MV high-voltage cryo-EM (cryo-HVEM) for single particle structural analysis (SPA) of giant viruses using tokyovirus, a species of Marseilleviridae, and revealed the capsid structure at 7.7 Å resolution. The capsid enclosing the viral DNA consisted primarily of four layers: (1) major capsid proteins (MCPs) and penton proteins, (2) minor capsid proteins (mCPs), (3) scaffold protein components (ScPCs), and (4) internal membrane. The mCPs showed a novel capsid lattice consisting of eight protein components. ScPCs connecting the icosahedral vertices supported the formation of the membrane extrusions, and possibly act like tape measure proteins reported in other giant viruses. The density on top of the MCP trimer was suggested to include glycoproteins. This is the first attempt at cryo-HVEM SPA. We found the primary limitations to be the lack of automated data acquisition and software support for collection and processing and thus achievable resolution. However, the results pave the way for using cryo-HVEM for structural analysis of larger biological specimens.
Digitalization impacts the COVID-19 pandemic and the stringency of government measures
Authors: Heinrichs Helen , Mueller Florian , Rohfleisch Lucia , Schulz Volkmar , Talbot Steven R. , Kiessling Fabian 
Published: December 2022

AbstractCOVID-19 poses a significant burden to populations worldwide. Although the pandemic has accelerated digital transformation, little is known about the influence of digitalization on pandemic developments. Therefore, this country-level study aims to explore the impact of pre-pandemic digital adoption on COVID-19 outcomes and government measures. Using the Digital Adoption Index (DAI), we examined the association between countries' digital preparedness levels and COVID-19 cases, deaths, and stringency indices (SI) of government measures until March 2021. Gradient Tree Boosting based algorithm pinpointed essential features related to COVID-19 trends, such as digital adoption, populations' smoker fraction, age, and poverty. Subsequently, regression analyses indicated that higher DAI was associated with significant declines in new cases (β = − 362.25/pm; p < 0.001) and attributed deaths (β = − 5.53/pm; p < 0.001) months after the peak. When plotting DAI against the SI normalized for the starting day, countries with higher DAI adopted slightly more stringent government measures (β = 4.86; p < 0.01). Finally, a scoping review identified 70 publications providing valuable arguments for our findings. Countries with higher DAI before the pandemic show a positive trend in handling the pandemic and facilitate the implementation of more decisive governmental measures. Further distribution of digital adoption may have the potential to attenuate the impact of COVID-19 cases and deaths.