bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wind‐Modulated Western Maine Coastal Current and Its Connectivity With the Eastern Maine Coastal Current
Authors: Li Denghui , Wang Zhengui , Xue Huijie , Thomas Andrew C. , Etter Ron J. 
Published: June 2022

AbstractUsing a high‐resolution circulation model and an offline particle tracking model, we investigated variations of the Western Maine Coastal Current (WMCC) and its connectivity with the Eastern Maine Coastal Current (EMCC). The models showed that the weak, broad, and sinuous WMCC is generally southwestward with an offshore and a nearshore core, fed by the extension of the EMCC and runoff from the Penobscot and Kennebec–Androscoggin Rivers, respectively. A sea‐level dome can form offshore of Casco Bay in late fall and early winter as the northeastward alongshore wind sets up a seaward sea‐level gradient from the coast to meet the shoreward sea‐level gradient from Wilkinson Basin. Consequently, northeastward flows (i.e., the counter‐WMCC) emerge on the inshore side of the dome. Both the circulation and particle tracking models suggested that the connectivity generally peaks twice annually, highest in winter and then secondarily in late spring or early summer. The former is concurrent with the most southwest offshore veering of the EMCC, while the latter is concurrent with the strongest EMCC. Moreover, the counter‐WMCC can reduce the connectivity and result in year‐to‐year variations.
A New Approach to Skillful Seasonal Prediction of Southeast Asia Tropical Cyclone Occurrence
Authors: Feng Xiangbo , Hodges Kevin I. , Hoang Lam , Pura Alvin G. , Yang Gui‐Ying , Luu Huyen , David Shirley J. , Duran Ger Anne M. W. , Guo Yi‐Peng 
Published: June 2022

AbstractPredicting the peak‐season (July–September) tropical cyclones (TCs) in Southeast Asia (SEA) several months ahead remains challenging, related to limited understanding and prediction of the dynamics affecting the variability of SEA TC activity. Here, we introduce a new statistical approach to sequentially identify mutually independent predictors for the occurrence frequency of peak‐season TCs in the South China Sea (SCS) and east of the Philippines (PHL). These predictors, which are identified from the preseason (April‐June) environmental fields, can capture the interannual variability of different clusters of peak‐season TCs, through a cross‐season effect on large‐scale environment that governs TC genesis and track. The physically oriented approach provides a skillful seasonal prediction in the 41‐year period (1979–2019), with r = 0.73 and 0.54 for SCS and PHL TC frequency, respectively. The lower performance for PHL TCs is likely related to the nonstationarity of the cross‐season TC‐environment relationship. We further develop the statistical approach to a hybrid method using the predictors derived from dynamical seasonal forecasts. The hybrid prediction shows a significant skill for both SCS and PHL TCs, for lead times up to four or 5 months ahead, related to the good performance of models for the sea surface temperatures and low‐level winds in the tropics. The statistical and hybrid predictions outperform the dynamical predictions, showing the potential for operational use.
Revisiting the Pan Evaporation Trend in China During 1988–2017
Authors: Shen Jiaju , Yang Hanbo , Li Sien , Liu Ziwei , Cao Yongqiang , Yang Dawen 
Published: June 2022

AbstractPan evaporation decrease has been reported worldwide over the past decades. A recent increasing pan evaporation trend has been found globally. Remarkably, most studies on Chinese pan evaporation change were based on simulations involving meteorological variables, including temperature, radiation (sunshine duration [SSD]), wind speed, and relative humidity (RH), due to the pan evaporation observation inconsistency caused by the micro‐pan (D20) replacement with large pan (E601) around 2002. In addition, a large‐scale humidity sensor replacement across China occurred since the 2000s can cause an inconsistency of RH and in turn lead to that of simulated pan evaporation. Therefore, the recent pan evaporation trends independent of the observed RH in China should be revisited. In this study, we (a) generate merged D20 pan evaporation from 1988 to 2017 according to E601 observations under the constant conversion coefficient assumption between the evaporation observations of these pans in the same month of every year at each station, and (b) propose a framework based on the PenPan‐D20 model to attribute pan evaporation change into specific humidity (instead of RH or vapor pressure deficit in previous studies), SSD, air temperature, and wind speed, since RH and vapor pressure deficit are not independent from air temperature. The results show a significant 2.68 mm/a/a upward pan evaporation trend (p < 0.05) across China from 1988 to 2017, which was primarily driven by the rising air temperature. Humidity sensor replacement causes an ∼2.2% inconsistency in RH, which produces nonnegligible errors in simulated pan evaporation trend.
CircRFWD3 promotes HNSCC metastasis by modulating miR-27a/b/PPARγ signaling
Authors: Wei Zihao , Wang Ying , Peng Jiakuan , Li Honglin , Gu Junjie , Ji Ning , Li Taiwei , Zhou Xikun , Zeng Xin , Li Jing , Chen Qianming 
Published: June 2022

AbstractHead and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer in the world, the 5-year survival rate of patients with HNSCC is still about 50% due to frequent metastasis and recurrence. Circular RNAs (circRNAs) have been characterized as key regulators of gene expression in numerous malignancies. However, the role of circRNA in HNSCC metastasis remains largely unknown. Here, we demonstrated that the circRFWD3 was significantly upregulated in HNSCC tissues and cell lines by circRNA microarray analysis and qPCR. Notably, high expression of circRFWD3 is related to highly aggressive HNSCC cell lines and lymph node metastasis in HNSCC patients. After that, Sanger sequencing, RNase R, and actinomycin D assay were performed to verify the ring structure of circRFWD3. Then functional experiments found it could promote the metastasis of HNSCC cells both in vitro and in vivo. Mechanistically, a dual-luciferase reporter assay, FISH, RIP, RNA pull-down, RNA-seq, and western blot experiments were employed and found that circRFWD3 served as a miRNAs sponge for miR-27a/27b, leading to the upregulation of PPARγ, and then promoted HNSCC metastasis via NF-κB/MMP13 pathway. Finally, ISH and IHC were carried out to determine the expression levels and clinical significances of circRFWD3 and PPARγ in clinical cohorts of HNSCC. According to the analysis results from two independent HNSCC cohorts, upregulated expression of circRFWD3 and PPARγ were positively associated with worse survival in patients with HNSCC. Overall, our results uncover that circRFWD3 acts a critical role in promoting the aggressiveness of HNSCC cells and is a prognostic marker for the disease, indicating that circRFWD3 may act as a potential therapeutic target in HNSCC.
Caspase-2 is a mediator of apoptotic signaling in response to gemtuzumab ozogamicin in acute myeloid leukemia
Authors: Hååg Petra , Olsson Magnus , Forsberg Jeremy , Lindberg Marita Lagergren , Stenerlöw Bo , Zong Dali , Kanter Lena , Lewensohn Rolf , Viktorsson Kristina , Zhivotovsky Boris , Stenke Leif 
Published: June 2022

AbstractThe antibody conjugate gemtuzumab ozogamicin (GO; Mylotarg®) provides targeted therapy of acute myeloid leukemia (AML), with recent approvals for patients with CD33-positive disease at diagnosis or relapse, as monotherapy or combined with chemotherapeutics. While its clinical efficacy is well documented, the molecular routes by which GO induces AML cell death warrant further analyses. We have earlier reported that this process is initiated via mitochondria-mediated caspase activation. Here we provide additional data, focusing on the involvement of caspase-2 in this mechanism. We show that this enzyme plays an important role in triggering apoptotic death of human AML cells after exposure to GO or its active moiety calicheamicin. Accordingly, the caspase-2 inhibitor z-VDVAD-fmk reduced GO-induced caspase-3 activation. This finding was validated with shRNA and siRNA targeting caspase-2, resulting in reduced caspase-3 activation and cleavage of poly [ADP-ribose] polymerase 1 (PARP-1). We previously demonstrated that GO-induced apoptosis included a conformational change of Bax into a pro-apoptotic state. Present data reveal that GO-treatment also induced Bid cleavage, which was partially reduced by caspase-2 specific inhibition while the effect on GO-induced Bax conformational change remained unaltered. In mononuclear cells isolated from AML patients that responded to GO treatment in vitro, processing of caspase-2 was evident, whereas in cells from an AML patient refractory to treatment no such processing was seen. When assessing diagnostic samples from 22 AML patients, who all entered complete remission (CR) following anthracycline-based induction therapy, and comparing patients with long versus those with short CR duration no significant differences in baseline caspase-2 or caspase-3 full-length protein expression levels were found. In summary, we demonstrate that GO triggers caspase-2 cleavage in human AML cells and that the subsequent apoptosis of these cells in part relies on caspase-2. These findings may have future clinical implications.
The combination of hydroxychloroquine and 2-deoxyglucose enhances apoptosis in breast cancer cells by blocking protective autophagy and sustaining endoplasmic reticulum stress
Authors: Zhou Ning , Liu Qingyun , Wang Xiao , He Lixin , Zhang Tao , Zhou Han , Zhu Xinying , Zhou Tianhong , Deng Ganzhen , Qiu Changwei 
Published: June 2022

Abstract2-Deoxyglucose (2-DG) can be used in antitumour research by inhibiting glycolysis and promoting the endoplasmic reticulum stress (ERS) pathway, but its clinical application is restricted due to dose-limiting side effects and survival chance for cancer cells by protective autophagy. Therefore, our research explored whether the combination of hydroxychloroquine (HCQ), an FDA-approved autophagy inhibiting drug, and 2-DG is a promising therapeutic strategy. Here, we report that HCQ combined with 2-DG can further inhibit the viability and migration and induce apoptosis of breast tumour cells compared with other individual drugs. The combination of 2-DG and HCQ can significantly reduce transplanted tumour size and tumour cell metastasis of the lung and liver in vivo. At the cellular level, HCQ suppressed autolysosome formation and terminated the autophagy process induced by 2-DG-mediated ERS, resulting in the continuous accumulation of misfolded proteins in the endoplasmic reticulum, which generated sustained ERS through the PERK-eIF2α-ATF-4-CHOP axis and triggered the transformation from a survival process to cell death. Our research reinforced the research interest of metabolic disruptors in triple-negative breast cancer and emphasized the potential of the combination of 2-DG and HCQ as an anticancerous treatment.
Photon-free (s)CMOS camera characterization for artifact reduction in high- and super-resolution microscopy
Authors: Diekmann Robin , Deschamps Joran , Li Yiming , Deguchi Takahiro , Tschanz Aline , Kahnwald Maurice , Matti Ulf , Ries Jonas 
Published: June 2022

AbstractModern implementations of widefield fluorescence microscopy often rely on sCMOS cameras, but this camera architecture inherently features pixel-to-pixel variations. Such variations lead to image artifacts and render quantitative image interpretation difficult. Although a variety of algorithmic corrections exists, they require a thorough characterization of the camera, which typically is not easy to access or perform. Here, we developed a fully automated pipeline for camera characterization based solely on thermally generated signal, and implemented it in the popular open-source software Micro-Manager and ImageJ/Fiji. Besides supplying the conventional camera maps of noise, offset and gain, our pipeline also gives access to dark current and thermal noise as functions of the exposure time. This allowed us to avoid structural bias in single-molecule localization microscopy (SMLM), which without correction is substantial even for scientific-grade, cooled cameras. In addition, our approach enables high-quality 3D super-resolution as well as live-cell time-lapse microscopy with cheap, industry-grade cameras. As our approach for camera characterization does not require any user interventions or additional hardware implementations, numerous correction algorithms that rely on camera characterization become easily applicable.
High critical current density and high-tolerance superconductivity in high-entropy alloy thin films
Authors: Jung Soon-Gil , Han Yoonseok , Kim Jin Hee , Hidayati Rahmatul , Rhyee Jong-Soo , Lee Jung Min , Kang Won Nam , Choi Woo Seok , Jeon Hye-Ran , Suk Jaekwon , Park Tuson 
Published: June 2022

AbstractHigh-entropy alloy (HEA) superconductors—a new class of functional materials—can be utilized stably under extreme conditions, such as in space environments, owing to their high mechanical hardness and excellent irradiation tolerance. However, the feasibility of practical applications of HEA superconductors has not yet been demonstrated because the critical current density (Jc) for HEA superconductors has not yet been adequately characterized. Here, we report the fabrication of high-quality superconducting (SC) thin films of Ta–Nb–Hf–Zr–Ti HEAs via a pulsed laser deposition. The thin films exhibit a large Jc of >1 MA cm−2 at 4.2 K and are therefore favorable for SC devices as well as large-scale applications. In addition, they show extremely robust superconductivity to irradiation-induced disorder controlled by the dose of Kr-ion irradiation. The superconductivity of the HEA films is more than 1000 times more resistant to displacement damage than that of other promising superconductors with technological applications, such as MgB2, Nb3Sn, Fe-based superconductors, and high-Tc cuprate superconductors. These results demonstrate that HEA superconductors have considerable potential for use under extreme conditions, such as in aerospace applications, nuclear fusion reactors, and high-field SC magnets.
Enhancing bioreactor arrays for automated measurements and reactive control with ReacSight
Authors: Bertaux François , Sosa-Carrillo Sebastián , Gross Viktoriia , Fraisse Achille , Aditya Chetan , Furstenheim Mariela , Batt Gregory 
Published: June 2022

AbstractSmall-scale, low-cost bioreactors provide exquisite control of environmental parameters of microbial cultures over long durations. Their use is gaining popularity in quantitative systems and synthetic biology. However, existing setups are limited in their measurement capabilities. Here, we present ReacSight, a strategy to enhance bioreactor arrays for automated measurements and reactive experiment control. ReacSight leverages low-cost pipetting robots for sample collection, handling and loading, and provides a flexible instrument control architecture. We showcase ReacSight capabilities on three applications in yeast. First, we demonstrate real-time optogenetic control of gene expression. Second, we explore the impact of nutrient scarcity on fitness and cellular stress using competition assays. Third, we perform dynamic control of the composition of a two-strain consortium. We combine custom or chi.bio reactors with automated cytometry. To further illustrate ReacSight’s genericity, we use it to enhance plate-readers with pipetting capabilities and perform repeated antibiotic treatments on a bacterial clinical isolate.
Antigen glycosylation regulates efficacy of CAR T cells targeting CD19
Authors: Heard Amanda , Landmann Jack H. , Hansen Ava R. , Papadopolou Alkmini , Hsu Yu-Sung , Selli Mehmet Emrah , Warrington John M. , Lattin John , Chang Jufang , Ha Helen , Haug-Kroeper Martina , Doray Balraj , Gill Saar , Ruella Marco , Hayer Katharina E. , Weitzman Matthew D. , Green Abby M. , Fluhrer Regina , Singh Nathan 
Published: June 2022

AbstractWhile chimeric antigen receptor (CAR) T cells targeting CD19 can cure a subset of patients with B cell malignancies, most patients treated will not achieve durable remission. Identification of the mechanisms leading to failure is essential to broadening the efficacy of this promising platform. Several studies have demonstrated that disruption of CD19 genes and transcripts can lead to disease relapse after initial response; however, few other tumor-intrinsic drivers of CAR T cell failure have been reported. Here we identify expression of the Golgi-resident intramembrane protease Signal peptide peptidase-like 3 (SPPL3) in malignant B cells as a potent regulator of resistance to CAR therapy. Loss of SPPL3 results in hyperglycosylation of CD19, an alteration that directly inhibits CAR T cell effector function and suppresses anti-tumor cytotoxicity. Alternatively, over-expression of SPPL3 drives loss of CD19 protein, also enabling resistance. In this pre-clinical model these findings identify post-translational modification of CD19 as a mechanism of antigen escape from CAR T cell therapy.
Impact of extra-anatomical bypass on coarctation fluid dynamics using patient-specific lumped parameter and Lattice Boltzmann modeling
Authors: Sadeghi Reza , Tomka Benjamin , Khodaei Seyedvahid , Daeian MohammadAli , Gandhi Krishna , Garcia Julio , Keshavarz-Motamed Zahra 
Published: June 2022

AbstractAccurate hemodynamic analysis is not only crucial for successful diagnosis of coarctation of the aorta (COA), but intervention decisions also rely on the hemodynamics assessment in both pre and post intervention states to minimize patient risks. Despite ongoing advances in surgical techniques for COA treatments, the impacts of extra-anatomic bypass grafting, a surgical technique to treat COA, on the aorta are not always benign. Our objective was to investigate the impact of bypass grafting on aortic hemodynamics. We investigated the impact of bypass grafting on aortic hemodynamics using a patient-specific computational-mechanics framework in three patients with COA who underwent bypass grafting. Our results describe that bypass grafting improved some hemodynamic metrics while worsened the others: (1) Doppler pressure gradient improved (decreased) in all patients; (2) Bypass graft did not reduce the flow rate substantially through the COA; (3) Systemic arterial compliance increased in patients #1 and 3 and didn’t change (improve) in patient 3; (4) Hypertension got worse in all patients; (5) The flow velocity magnitude improved (reduced) in patient 2 and 3 but did not improve significantly in patient 1; (6) There were elevated velocity magnitude, persistence of vortical flow structure, elevated turbulence characteristics, and elevated wall shear stress at the bypass graft junctions in all patients. We concluded that bypass graft may lead to pseudoaneurysm formation and potential aortic rupture as well as intimal hyperplasia due to the persistent abnormal and irregular aortic hemodynamics in some patients. Moreover, post-intervention, exposures of endothelial cells to high shear stress may lead to arterial remodeling, aneurysm, and rupture.
Upregulation of microRNA-96-5p is associated with adolescent idiopathic scoliosis and low bone mass phenotype
Authors: Chen Huanxiong , Yang Kenneth Guangpu , Zhang Jiajun , Cheuk Ka-yee , Nepotchatykh Evguenia , Wang Yujia , Hung Alec Lik-hang , Lam Tsz-ping , Moreau Alain , Lee Wayne Yuk-wai 
Published: June 2022

AbstractBone densitometry revealed low bone mass in patients with adolescent idiopathic scoliosis (AIS) and its prognostic potential to predict curve progression. Recent studies showed differential circulating miRNAs in AIS but their diagnostic potential and links to low bone mass have not been well-documented. The present study aimed to compare miRNA profiles in bone tissues collected from AIS and non-scoliotic subjects, and to explore if the selected miRNA candidates could be useful diagnostic biomarkers for AIS. Microarray analysis identified miR-96-5p being the most upregulated among the candidates. miR-96-5p level was measured in plasma samples from 100 AIS and 52 healthy girls. Our results showed significantly higher plasma levels of miR-96-5p in AIS girls with an area under the curve (AUC) of 0.671 for diagnostic accuracy. A model that was composed of plasma miR-96-5p and patient-specific parameters (age, body weight and years since menarche) gave rise to an improved AUC of 0.752. Ingenuity Pathway Analysis (IPA) indicated functional links between bone metabolic pathways and miR-96-5p. In conclusion, differentially expressed miRNAs in AIS bone and plasma samples represented a new source of disease biomarkers and players in AIS etiopathogenesis, which required further validation study involving AIS patients of both genders with long-term follow-up.
Real-world experience of angiotensin receptor neprilysin inhibitor on the glucose-lowering effect
Authors: Kim Heungjo , Park Gyunam , Hahn Jongsung , Oh Jaewon , Chang Min Jung 
Published: June 2022

AbstractWe investigated the effect of angiotensin receptor neprilysin inhibitor (ARNI) on glycemic control in Korean patients. This retrospective cohort study was conducted at a single tertiary hospital. We compared the HbA1c level reduction between the ARNI and angiotensin-converting enzyme inhibitors (ACEIs) or angiotensin II receptor blockers (ARBs) in chronic heart failure patients with diabetes. We also examined whether the target HbA1c level was reached and the time to start insulin between the two groups. Over the study period, ARNI did not significantly lower the HbA1c level after adjusting confounding factors compared to ACEIs or ARBs. However, as a result of a simple comparison using Mann–Whitney U test, ARNI group showed significant decrease in HbA1c at 6, 12, and 24 months compared to ACEIs or ARBs group (p = 0.003, 0.009, and 0.026, respectively). The initiation of insulin was delayed in the ARNI group, but this difference was not significant based on the result of hazard ratio, but cumulative incidence was significantly lower in the ARNI group. In the real world, the blood glucose-control effects of ARNI were not superior to those of ACEIs or ARBs. However, long-term studies are needed as ARNI use increases to obtain more statistically significant results.
The new X-ray/visible microscopy MAXWELL technique for fast three-dimensional nanoimaging with isotropic resolution
Authors: Kohmura Yoshiki , Yang Shun-Min , Chen Hsiang-Hsin , Takano Hidekazu , Chang Chia-Ju , Wang Ya-Sian , Lee Tsung-Tse , Chiu Ching-Yu , Yang Kai-En , Chien Yu-Ting , Hu Huan-Ming , Su Tzu-Ling , Petibois Cyril , Chen Yi-Yun , Hsu Cheng-Huan , Chen Peilin , Hueng Dueng-Yuan , Chen Shean-Jen , Yang Chi Lin , Chin An-Lun , Low Chian-Ming , Tan Francis Chee Kuan , Teo Alvin , Tok Eng Soon , Cai Xu Xiang , Lin Hong-Ming , Boeckl John , Stampfl Anton P. , Yamada Jumpei , Matsuyama Satoshi , Ishikawa Tetsuya , Margaritondo Giorgio , Chiang Ann-Shyn , Hwu Yeukuang 
Published: June 2022

AbstractMicroscopy by Achromatic X-rays With Emission of Laminar Light (MAXWELL) is a new X-ray/visible technique with attractive characteristics including isotropic resolution in all directions, large-volume imaging and high throughput. An ultrathin, laminar X-ray beam produced by a Wolter type I mirror irradiates the sample stimulating the emission of visible light by scintillating nanoparticles, captured by an optical system. Three-dimensional (3D) images are obtained by scanning the specimen with respect to the laminar beam. We implemented and tested the technique with a high-brightness undulator at SPring-8, demonstrating its validity for a variety of specimens. This work was performed under the Synchrotrons for Neuroscience—an Asia–Pacific Strategic Enterprise (SYNAPSE) collaboration.