bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative proteomic analysis of cerebrospinal fluid reveals CD163, A2M and full-length APP as potential diagnostic biomarkers of paediatric bacterial meningitis
Authors: Luo Ting , Yang Sai , Chen Yan , Liu Shulei , Yang Liming , Hu Nanfei , Ma Ye , Qiu Jun , Wang Kewei , Li Liping , Tan Lihong 
Published: May 2022

Abstract
Background
Bacterial meningitis (BM) is a life-threatening infectious disease of the central nervous system in infants and children. To date, no diagnostic methods for the early and precise diagnosis of paediatric BM have been developed.

Methods
A label-free cerebrospinal fluid (CSF) quantitative proteomic analysis of 8 patients with confirmed or suspected BM, 9 patients with confirmed or suspected viral meningitis (VM) and 6 non-CNS-infected hospital patients was performed via high-resolution LC–MS/MS.

Results
Our CSF proteomic analysis allowed the identification of critical differences between the BM and non-BM groups. Compared to the proteomes of the non-BM groups, the proteome of the paediatric BM group was characterized by upregulation of complement and coagulation cascades, regulation of IGF transport, uptake by IGF-binding proteins and acute inflammatory response, downregulation of developmental growth, and metabolism of carbohydrates. Moreover, the levels of CD163, A2M and full-length APP in CSF showed excellent diagnostic performance for paediatric BM, with AUC values of 0.911 (95% CI: 0.839–0.984), 0.908 (95% CI: 0.816–1.000) and 0.944 (95% CI: 0.86, 1.000), respectively. Among them, A2M and full-length APP are reported here for the first time as potential diagnostic biomarkers of BM. The findings imply that peptidase regulator activity plays an important role in BM and provide potential novel targets for precision medicine in paediatric BM.

Conclusions
CD163, A2M and full-length APP are validated as potential diagnostic biomarkers of paediatric BM.
The role of systemic immuno-inflammatory factors in resectable pancreatic adenocarcinoma: a cohort retrospective study
Authors: Schlanger D. , Popa C. , Pașca S. , Seicean A. , Al Hajjar N. 
Published: May 2022

Abstract
Background
Pancreatic cancer is an aggressive malignancy, surgery being the only potentially curative treatment. The systemic inflammatory response is an important factor in the development of cancer. There is still controversy regarding its role in pancreatic cancer.

Methods
Our study is a retrospective observational cohort study. We included patients diagnosed with pancreatic ductal adenocarcinoma (PDAC), who underwent surgical resection in our hospital, between January 2012 and December 2019. We gathered information from preoperative and postoperative blood tests. Neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), lymphocyte to monocyte ratio (LMR), prognostic nutritional index (PNI) and systemic immune-inflammation index (SII) were determined.

Results
We included 312 patients. All the immune-inflammatory scores assessed significantly changed after the surgery. The impact on overall survival of these markers showed that only some of the postoperative scores predicted survival: high PLR had a negative prognostic impact, while high lymphocyte and PNI values had a positive effect on overall survival.

Discussion
The circulating immune cells and their values integrated in the assessed prognostic scores suffer statistically significant changes after curative pancreatic surgery. Only the postoperative values of lymphocyte count, PLR, and PNI seem to influence the overall survival in PDAC.

Trial registration
ClinicalTrials.gov–identifier NCT05025371.
Growth differentiation factor 11 induces skeletal muscle atrophy via a STAT3-dependent mechanism in pulmonary arterial hypertension
Authors: Xiang Guiling , Ying Kelu , Jiang Pan , Jia Mengping , Sun Yipeng , Li Shanqun , Wu Xiaodan , Hao Shengyu 
Published: May 2022

AbstractSkeletal muscle wasting is a clinically remarkable phenotypic feature of pulmonary arterial hypertension (PAH) that increases the risk of mortality. Growth differentiation factor 11 (GDF11), centrally involved in PAH pathogenesis, has an inhibitory effect on skeletal muscle growth in other conditions. However, whether GDF11 is involved in the pathogenesis of skeletal muscle wasting in PAH remains unknown. We showed that serum GDF11 levels in patients were increased following PAH. Skeletal muscle wasting in the MCT-treated PAH model is accompanied by an increase in circulating GDF11 levels and local catabolic markers (Fbx32, Trim63, Foxo1, and protease activity). In vitro GDF11 activated phosphorylation of STAT3. Antagonizing STAT3, with Stattic, in vitro and in vivo, could partially reverse proteolytic pathways including STAT3/socs3 and iNOS/NO in GDF11-meditated muscle wasting. Our findings demonstrate that GDF11 contributes to muscle wasting and the inhibition of its downstream molecule STAT3 shows promise as a therapeutic intervention by which muscle atrophy may be directly prevented in PAH.
Whole genome sequencing and analysis of fenvalerate degrading bacteria Citrobacter freundii CD-9
Authors: Zhou Xuerui , Lei Dan , Tang Jie , Wu Min , Ye Hong , Zhang Qing 
Published: May 2022

AbstractCitrobacter freundii CD-9 is a Gram-negative bacteria sourced from factory sludge that can use fenvalerate as its sole carbon source and has a broad degradation spectrum for pyrethroid pesticides. The whole genome of CD-9 sequenced using Illumina HiSeq PE150 was reported in this study. The CD-9 genome size was 5.33 Mb and the G + C content was 51.55%. A total of 5291 coding genes, 9 5s-rRNA, and 79 tRNA were predicted bioinformatically. 3586 genes annotated to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database that can be involved in 173 metabolic pathways, including various microbial metabolic pathways that degrade exogenous chemicals, especially those that degrade aromatic compounds, and also produce a variety of bioactive substances. Fifty genes related to pyrethroid degradation were identified in the C. freundii CD-9 genome, including 9 dioxygenase, 25 hydrolase, and 16 esterase genes. Notably, RT-qPCR results showed that from the predicted 13 genes related to fenvalerate degradation, the expression of six genes, including esterase, HAD family hydrolase, lipolytic enzyme, and gentisic acid dioxygenase, was induced in the presence of fenvalerate. In this study, the key genes and degradation mechanism of C. freundii CD-9 were analyzed and the results provide scientific evidence to support its application in environmental bioremediation. It can establish application models for different environmental pollution management by constructing genetically engineered bacteria for efficient fenvalerate or developing enzyme formulations that can be industrially produced.
How to avoid unjust energy transitions: insights from the Ruhr region
Authors: Arora Anmol , Schroeder Heike 
Published: May 2022

Abstract
Background
The transition of the Ruhr region in Germany from a hard coal belt into a knowledge-based economy with a dynamic service sector and state of the art universities over the past 60–80 years has been widely touted as a successful example of how just and fair low carbon energy transitions can unfold.

Methods
This paper leverages documentary analysis of data across a wide array of sources to test these claims and identify lessons by creating a novel just energy transition framework.

Results
The study finds economic motivation, mindset and reorientation—not environmental concerns—to be the defining features for at least the first two decades of this shift. The lack of willingness to acknowledge environmental impacts and market realities has delayed the transition and led to wasteful allocation of resources towards supporting the hard coal mining industry. The prominence given to distributional justice cushions the victims of this transition financially, but does not allow a broad based coalition to advance the transition process. It is in the second phase (mid-1980s onwards that we see procedural aspects of justice come forth and support greater ownership and sustainability of the transition to emerge, while the evidence of recognition justice continues to be scant.

Conclusions
There are many nuanced successes in the Ruhr’s example, along with some failures worth highlighting. It is in the breakdown of this transition into two distinct phases and their nuances (particularly in the domain of justice) that fresh insights emerge and allow for a better understanding of what constitutes a suitable energy transition in a particular socio-economic and political context. As the international community embarks on ambitious greenhouse gas reduction targets, it can maximise the benefits and minimise the damages and costs by considering these realities on the ground.
Antimicrobial resistance in Antarctica: is it still a pristine environment?
Authors: Hwengwere K. , Paramel Nair H. , Hughes K. A. , Peck L. S. , Clark M. S. , Walker C. A. 
Published: May 2022

AbstractAlthough the rapid spread of antimicrobial resistance (AMR), particularly in relation to clinical settings, is causing concern in many regions of the globe, remote, extreme environments, such as Antarctica, are thought to be relatively free from the negative impact of human activities. In fact, Antarctica is often perceived as the last pristine continent on Earth. Such remote regions, which are assumed to have very low levels of AMR due to limited human activity, represent potential model environments to understand the mechanisms and interactions underpinning the early stages of evolution, de novo development, acquisition and transmission of AMR. Antarctica, with its defined zones of human colonisation (centred around scientific research stations) and large populations of migratory birds and animals, also has great potential with regard to mapping and understanding the spread of early-stage zoonotic interactions. However, to date, studies of AMR in Antarctica are limited. Here, we survey the current literature focussing on the following:Dissection of human-introduced AMR versus naturally occurring AMR, based on the premise that multiple drug resistance and resistance to synthetic antibiotics not yet found in nature are the results of human contaminationThe potential role of endemic wildlife in AMR spreadThere is clear evidence for greater concentrations of AMR around research stations, and although data show reverse zoonosis of the characteristic human gut bacteria to endemic wildlife, AMR within birds and seals appears to be very low, albeit on limited samplings. Furthermore, areas where there is little, to no, human activity still appear to be free from anthropogenically introduced AMR. However, a comprehensive assessment of AMR levels in Antarctica is virtually impossible on current data due to the wide variation in reporting standards and methodologies used and poor geographical coverage. Thus, future studies should engage directly with policymakers to promote the implementation of continent-wide AMR reporting standards. The development of such standards alongside a centralised reporting system would provide baseline data to feedback directly into wastewater treatment policies for the Antarctic Treaty Area to help preserve this relatively pristine environment.
Does maxillomandibular fixation affect skeletal stability following mandibular advancement? A single-blind clinical trial
Authors: Tabrizi Reza , Sarrafzadeh Arash , Shafiei Shervin , Moslemi Hamidreza , Dastgir Ramtin 
Published: May 2022

Abstract
Background
The stability of the results remains a significant concern in orthognathic surgeries. This study aimed to assess the amount of relapse following mandibular advancement with/without maxillomandibular fixation (MMF).

Materials and methods
A single-blind clinical trial was conducted on patients with mandibular retrognathism who underwent BSSO for mandibular advancement and Lefort I maxillary superior repositioning. Patients were randomly divided into two groups of treatment (MMF) and control (no MMF). In the treatment group, MMF was performed for 2 weeks; meanwhile, MMF was not performed in the control group, and only guiding elastics were applied postoperatively. Lateral cephalograms were obtained preoperatively (T1), immediately after surgery (T2), and at 1 year postoperatively (T3). The distance from points A and B to the X and Y plane were measured to identify the amount of vertical and horizontal relapse in 1 year as a primary outcome. An independent t-test was applied in order to find differences in outcomes between the control and treatment groups.

Results
Fifty-eight patients were evaluated in two groups (28 patients in the MMF group and 30 in the no-MMF group). The magnitude of mandibular advancement following BSSO was 7.68±1.39 mm and 7.53±1.28, respectively, without significant difference among the groups (p= 0.68). The mean sagittal and vertical changes (relapse) at point B were significantly different between the two groups at 1-year follow-up after the osteotomy (p=0.001 and p=0.05, respectively).

Conclusion
According to the results of this study, patients with short-term MMF following BSSO for mandibular advancement benefit from significantly greater skeletal stability in the sagittal and vertical dimensions.
IVF-conceived children outcome from birth to 3 years at a single IVF center in Indonesia: frozen versus fresh embryo transfer
Authors: Muharam R. , Umami Erda Ayu , Gunardi Hartono 
Published: May 2022

Abstract
Background
Frozen embryo transfer procedure is becoming more common in assisted reproduction technology. The effect of this technology is still not yet well studied in developing countries with limited resources, including Indonesia. This study explores the clinical outcomes between frozen and fresh embryo transfer-conceived children aged 0–3 years in Indonesia.

Results
The participants were divided into frozen embryo (n = 30) and fresh embryo (n = 30) transfer groups. There were no differences in growth and development. However, in vitro fertilization (IVF) children with frozen embryo transfer had a lower risk of developing low birth weight compared to the fresh embryo group with an OR of 0.17 (95% CI: 0.03–0.85). All children, both in the fresh and frozen embryos, have normal development.

Conclusions
Frozen versus fresh embryo transfer does not affect child growth and development.
Should asymptomatic small anteriorly located midline prostatic cyst in young adults be treated surgically or conservatively? A case report and review of the literature
Authors: Elsorougy Ali , Farg Hashim , Abdelhamid Abdalla , Zakaria Mostafa , El-Ghar Mohamed Abou 
Published: May 2022

Abstract
Background
Prostatic cysts are uncommon, typically asymptomatic and discovered by chance during imaging. Prostatic cysts in the midline are less prevalent and primarily seen in the posterior aspect of the prostate.

Case presentation
We describe a case of a 32-year-old man with a complaint of left loin pain and a little sensation of pelvic discomfort. Ultrasound was done revealing small pelvic cystic structure related to urinary bladder base and neck with possibility to be prostatic in origin. Transrectal ultrasound and pelvic magnetic resonance imaging were done and showed an anteriorly located midline prostatic cyst protruding into the bladder lumen, with no communication with the urethra on conventional ascending urethrogram.

Conclusion
The rare relationship between the cyst, bladder neck, and prostate make this case to some extent unique and further interesting. To our best knowledge, this is the eighth documented case in the literature to describe an anteriorly located midline intraprostatic cyst projecting at the bladder neck and base with illustration of different plans of treatment.
FDG-PET/CT tumor to liver SUV ratio (TLR), tumor SUVmax, and tumor size: can this help in differentiating squamous cell carcinoma from adenocarcinoma of the lung?
Authors: Salem Amr Muhammad Abdo , Hussein Laila Hosny , Osman Ahmed M. 
Published: May 2022

Abstract
Background
PET/CT plays an essential role in the diagnosis, staging, and follow-up of lung cancer. We aimed to assess the ability of PET/CT to differentiate between adenocarcinomas (AC) and squamous cell carcinomas (SCC) of the lung using tumor size, tumor maximum standardized uptake value (SUVmax), lymph nodes SUVmax, and tumor to liver SUV ratio (TLR).

Results
A total of 60 patients pathologically proved to have non-small cell lung cancer either AC or SCC were retrospectively evaluated. The mean tumor size, SUVmax of the tumor, and TLR were significantly higher in SCC lesions compared to AC lesions. The mean SCC tumoral size was 7.96 ± 2.18 cm compared to 5.66 ± 2.57 cm in AC lesions (P = 0.008). The mean tumor SUVmax in SCC lesions was 18.95 ± 8.3 compared to 12.4 ± 7.55 in AC lesions (P = 0.04). While the mean TLR of SCC lesions was 10.32 ± 4.03 compared to 7.36 ± 4.61 in AC lesions (P = 0.028). All three parameters showed the same sensitivity (75%), while TLR showed the highest specificity (77.78%) followed by tumor size (76.47%) and then SUVmax of the tumor (72.22%).

Conclusions
SCC of the lung has a higher mean tumor size, SUVmax of the tumor, and TLR as compared to AC which can be helpful tools in differentiation between them using PET/CT.
Expansion, Compression, Neither, Both? Divergent Patterns in Healthy, Disability-Free, and Morbidity-Free Life Expectancy Across U.S. Birth Cohorts, 1998–2016
Authors: Payne Collin F. 
Published: May 2022

Abstract
This article explores how patterns of health, morbidity, and disability have changed across successive generations of older adults in the United States. Using a novel method for comparing state-specific partial life expectancies—that is, total life expectancy (LE), and health expectancies (HEs) in different health states, bounded between two ages—I explore changes in healthy life expectancy across successive birth cohorts of the U.S. population. Results show that little compression of disability is occurring across cohorts, LE with chronic morbidities has expanded considerably, and self-rated health is improving across cohorts, but only at ages 70+. These findings suggest that successive cohorts in the U.S. population may be on divergent paths in terms of late-life health and well-being. Exploring heterogeneity in these patterns, I find that less educated individuals have substantially lower partial LE and disability-free LE than those with more schooling, and that disability-free life is declining among those with less than a high school diploma. Differences in HEs are pervasive across racial and ethnic groups, and both disabled LE and unhealthy LE are expanding in some disadvantaged subgroups. The continued increases in partial LE with morbidities across successive cohorts, and the broad stagnation of disability-free and healthy LE, present a broad view of a U.S. population in which successive generations are not living healthier lives.
“Common Informality”
Authors: Law Christopher 
Published: May 2022

AbstractThis article explores how two key problems of philosophical aesthetics, temporality and form, are rethought in Fred Moten's consent not to be a single being trilogy. The article proposes that Moten's work is notable for its refusal to affirm a link between aesthetic experience, or aesthesis, and the future-bound possibility of political community. This refusal distinguishes Moten's work both from the political philosophy underlying Immanuel Kant's aesthetics and from the dialectical critique of Kant found in contemporary theoretical work prioritizing formal experimentation. The article contends instead that Moten's work is shaped by a sustained attention to “informal” patterns of aesthetic experience, for which the graphic materiality of writing functions as a privileged index. The article then explores the political and temporal implications of writing's materiality in two essays from Moten's Black and Blur. To pursue this task, it draws on Walter Benjamin's understanding of philological interpolation and argues that Moten's work, particularly in its insistence on “renomination” rather than conceptual creation, can likewise be understood as philological. The article concludes, however, by showing how the idea of linguistic freedom advanced in recent philological work is complicated by Moten's recognition of a link between predication and blackness.
Aesthetics of (Black) Breathing
Authors: Cox Jessie , Jean-François Isaac 
Published: May 2022

Abstract
A meditation on (Black) breathing, this essay reflects on blackness and breathing as motile (un)representable practices. Black subjects are able to resist by breathing in spite of modernity's impulse to cut off black motion at the throat. Though deeply interested in the sounds and inner motions cohered at the head and neck, this essay understands breathing to affect matter and explores how breathing disarticulates wholeness as a practice of radically dissolving sovereign sound and form. The parentheses around (Black) attend to that which is founded on the erasure of Black liveness alongside such erasure, while also listening to those composers and aesthetes that are erased when we do not listen for their breathing. (Black) breathing is channeled into/through instruments, making it musical, and through these sonic transmutations of states of matter, possible futures are opened. The essay deploys an iterative play with punctuation and grammar to intensify the act and experience of writing alongside a chorus of voices both silenced and sounded. The entanglement of breathing, from past into future spaces, is what shifts a musical opening to a place of altered destiny. With black life cornered into visible places of antiblack state violence, in this piece we attempt to listen for, and breathe, another articulation of the world.
Determinants of patient behavioural loyalty on primary health centres: Evidence from a cross-sectional study in Indonesia
Authors: Mardaleta Mardaleta , Lubis Abdul Rahman , Diantimala Yossi , Fahlevi Heru 
Published: May 2022

Background: Patients’ loyalty to visit and use the services provided by the primary health centers (PHCs) is an important requirement of a patient referral system in many countries. The aim of this study was to examine the influence of internal service factors (service provider, service process, and service environment) on service quality and behavioural loyalty of patients in Indonesian PHCs. Methods: A cross-sectional study was conducted in 14 districts in Aceh Province, Indonesia between September and December 2020. Data were collected in 102 PHCs that were selected randomly from 137 PHCs that have an Inpatient Unit in the province. A proportional number of patients were recruited from each PHC and 389 patients were included. The demographic data, three components of internal service factors (service provider, service process, and service environment), the service quality and behavioural loyalty were assessed using a validated questionnaire. Hypothesis testing was conducted by using the structural equation model (SEM). Results: Our data suggested that two elements of internal service factors (service provider and service environment) had a positive and significant influence on service quality of the PHCs with p<0.001 and p=0.021, respectively. Service quality had a positive and significant influence of behavioural loyalty of patients to the PHCs (p=0.003). Service quality however did not serve as an intervening variable between internal service factors (service provider, service process, and service environment) and behavioural loyalty of patients, with p=0.091, p=0.230 and p=0.260, respectively. Conclusions: Service provider and service environment are two main factors that influence the service quality and the service quality directly influence the behavioural loyalty on PHC users. Therefore, to increase the patients’ loyalty to use the PHC services, the quality of the services should be improved by levelling up the quality of providers and both physical and social environments in the PHCs.