bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-34a/DRP-1-mediated mitophagy participated in cisplatin-induced ototoxicity via increasing oxidative stress
Authors: Wang Haiyan , Lin Hanqing , Kang Weibiao , Huang Lingfei , Gong Sisi , Zhang Tao , Huang Xiaotong , He Feinan , Ye Yongyi , Jia Haiying , Yang Haidi 
Published: September 2022

Abstract
Purpose
Cisplatin is a widely and effectively chemotherapy agent for most of solid malignant tumors. However,cisplatin induced ototoxicity is a common adverse effect,which limited the therapeutic efficacy of tumors in clinic.To date,the specific mechanism of ototoxicity is not fully elucidated and the management of cisplatin-induced ototoxicity also is an urgent challenge.Recently,some authors believed that miR34a and mitophagy played a role in age-related and drug-induced hearing loss.Our study is aimed at exploring the involvement of miR-34a/DRP-1 mediated mitophagy on cisplatin-induced ototoxicity.
Methods
In this study,C57BL/6 mices and HEI-OC1 cells were treated with cisplatin,miR-34a and DRP-1 level was analyzed by qRT-PCR and western blot,and assessed mitochondrial function via oxidative stress,JC-1 and ATP content.Subsequently,we detected DRP-1 level and observed mitochondrial function through modulating miR-34a expression in HEI-OC1 cells,to determine the effect of miR-34a on DRP-1 mediated mitophagy.
Results
MiR-34a expression increased and DRP-1 level decreased in C57BL/6 mice and HEI-OC1 cell treated with cisplatin,and mitochondria dysfunction was involved in the process.Furthermore,miR-34a mimic decreased DRP-1 expression,enhanced cisplatin-induced ototoxicity and aggravated mitochondrial dysfunction.We further verified that miR-34a inhibitor increased DRP-1 expression, partially protected aganist cisplatin-induced ototoxicity and improved mitochondrial function.
Conclusion
MiR-34a/DRP-1-mediated mitophagy was related to cisplatin induced ototoxicity and might be a novel target for investigating the treatement and protection of cisplatin-induced ototoxicity.
DT-CWT and Type-2 Fuzzy-HSAPF for Harmonic Compensation in Distribution System
Authors: Mishra Alok Kumar , Nanda Pradip Kumar , Ray Prakash Kumar , Das Soumya Ranjan , Patra Akshaya Kumar 
Published: September 2022

Abstract
Extensive utilization of power electronics in different applications injects harmonics into degrading the power quality. Harmonics can be eliminated in distribution systems using low-cost passive filters, but, they become bulky and complex in design with the increase in harmonic order. Hence, the active power filters (APFs) are proposed as a voltage source converter (VSC) for providing better compensation but, active filtering is costly. To minimize such demerits, this article represents a hybrid shunt active power filter (HSAPF). For reference current calculation, Dual Tree-Complex Wavelet Transform (DT-CWT) is used here and conventional proportional-integral (CPI), Type-1 and Type-2 Fuzzy Logic Controllers (T1FLC & T2FLC) are employed for parameter optimization of HSAPF, to enhance the harmonics compensation ability and power factor. The case studies are performed in both simulations as well as real-time experimental setup using dSPACE and it is analyzed that the proposed DT-CWT and Type-2 Fuzzy-based HSAPF provide better compensation performance in comparison to other controllers under different operating conditions.
A Novel Deep Reinforcement Learning Model for Resilient Road Network Recovery from Multiple Hazards
Authors: Fan Xudong , Zhang Xijin , Wang Xiaowei , Yu Xiong 
Published: September 2022

Abstract
As the backbone and the ‘blood vessel’ of modern cities, road networks provide critical support for community activities and economic growth, with their roles even more crucial due to the dramatic progress in urbanization. The service of road networks is subjected to the increasing frequency of high-consequence natural hazards such as earthquakes, floods, hurricanes, etc. Identifying resilient restoration sequences is essential to mitigate the disruption of such important infrastructure networks. This paper investigates a novel decision-support model to optimize post-disaster road network repair sequence. The model, named as GCN-DRL model, integrates the advantages of deep reinforced learning (DRL) with graph convolutional neural network (GCN), two emerging artificial intelligence (AI) techniques to achieve efficient recovery of road network service. The model is applied to analyze two cases of community road networks in the US that are subjected to different types of hazards, i.e., earthquakes and flooding. The performance of repair sequence by the GCN-DRL model is compared with two commonly used methods, i.e., repair sequence by the genetic algorithm and by prioritization based on graph importance with betweenness centrality. The results showed the decision sequence by GCN-DRL model consistently achieved superior performance in road network restoration than the conventional methods. The AI-based decision model also features high computational efficiency since the GCN-DRL model can be trained before the hazard. With a pre-trained GCN-DRL model, a close to optimal decision-making process can be made available rapidly for different types of new hazards, which is advantageous in efficiently responding to hazards when they happen. This study demonstrates the promise of a new AI-based decision support model to improve the resilience of road networks by enabling efficient post-hazards recovery.
Common mouse models of tauopathy reflect early but not late human disease
Authors: Wenger Kathrin , Viode Arthur , Schlaffner Christoph N. , Zalm Patrick van , Cheng Long , Dellovade Tammy , Langlois Xavier , Bannon Anthony , Connors Theresa R. , Oakley Derek , Renard Bernhard , Rappsilber Juri , Hyman Bradley , Steen Hanno , Steen Judith 
Published: September 2022

Abstract
Background:
Mouse models that overexpress human mutant Tau (P301S and P301L) are commonly used in preclinical studies of Alzheimer’s Disease (AD) and while several drugs showed therapeutic effects in these mice, they were ineffective in humans. This leads to the question to which extent the murine models reflect human Tau pathology on the molecular level.
Methods:
We isolated pathological Tau species from two common AD mouse models during different stages of disease and characterized the modification landscape of the aggregated Tau using targeted and untargeted mass spectrometry-based proteomics. The results were compared to human AD and to human carriers of the P301L Tau mutation that suffered from early onset dementia.
Results:
Both mouse models accumulate pathological Tau species during disease. The Tau aggregation is driven by progressive phosphorylation within the proline rich domaine and the C-terminus of the protein. This is reflective of early disease stages of human AD and of the pathology of human P301L carriers. However, Tau ubiquitination and acetylation, which are important to late-stage human AD are not represented in the mouse models.
Conclusion:
AD mouse models that overexpress human Tau using risk mutations are a suitable tool for testing drug candidates that aim to intervene in the early formation of pathological Tau species promoted by increased phosphorylation of Tau.
Characterization of the VRN-A1 allele introgressed from T. aestivum ssp. petropavlovskyi that influences the heading time in bread wheat
Authors: chumanova evgeniya , Efremova Tatyana , Vavilova Valeriya 
Published: September 2022

Abstract
The optimal timing of the heading stage, mainly determined by the VRN genes, contributes to the wide spread of bread wheat and the realization of adaptive and breeding potential. Wild wheat species are valuable sources for expanding the bread wheat genetic diversity by the introgression of new gene alleles, including VRN genes. In this study, a near-isogenic line of the winter wheat cultivar Bezostaya 1 with a VRN-A1 dominant allele was obtained with a T. aestivum ssp. petropavlovskyi accession as the donor. Using known PCR markers for the promoter and first intron sequences of the VRN-1 gene and subsequent sequencing of PCR fragments, the presence of the Langdon deletion was revealed in the first intron (Vrn-A1L allele), previously described only for tetraploid wheat. The allele composition of VRN genes was determined in T. aestivum ssp. petropavlovskyi accessions and the presence of the Vrn-A1L dominant allele was established in all accessions. It was shown that the Vrn-A1L dominant allele increased the shoots-heading period under long- and short-day conditions, which is associated with a prolongation before the first node formation. The comparative study of productivity characteristics of isogenic lines with Vrn-A1a and Vrn-A1Ldominant alleles on spike and plant productivity is presented.
The male-heterogametic sex determination system on chromosome 15 of Salix arbutifolia and Salix triandra reveals ancestral male heterogamety and subsequent turnover events in the genus Salix
Authors: He Li , Wang Yi , Cai Xinjie , Zhang Yue , Hörandl Elvira , Zhang Zhixiang 
Published: September 2022

Abstract
Dioecious Salix evolved more than 45 million years ago, but have homomorphic sex chromosomes, suggesting that turnover event(s) prevented major differentiation. Sex chromosome turnover events have been inferred in the sister genus, Populus. The genus Salix includes two main clades, Salix and Vetrix, with several previously studied Vetrix clade species having female-heterogametic (ZW) sex-determining systems (SDSs) on chromosome 15, while two Salix clade species have male-heterogametic (XY) SDSs on chromosome 7. We here studied two basal taxa of the Vetrix clade, S. arbutifolia and S. triandra. Analyses of whole genome resequencing data for genome-wide associations (GWAS) with the sexes and genetic differentiation between the sexes (FST values) showed that both species have male heterogamety with a sex-determining locus on chromosome 15, suggesting an early turnover event within the Vetrix clade, perhaps promoted by sexually antagonistic or (and) sex-ratio selection. Changepoint analysis based on FST values identified small sex-linked regions of ~3.33 Mb and ~2.80 Mb in S. arbutifolia and S. triandra, respectively. The molecular mechanism of sex-determination remains unknown. Ancestral state reconstruction of SDS and stamen numbers suggests that at least two turnover events occurred in Salix. Willows with XY SDS usually have multiple stamens, while ZW species have two stamens.
The association of severe COVID anxiety with poor social functioning, quality of life, and protective behaviours among adults in United Kingdom: A cross-sectional study.
Authors: King Jacob D , McQuaid Aisling , Leeson Verity C , Samuel Oluwaseun , Grant Josiah , Azeem Muhamad Saad Imran , Barnicot Kirsten , Crawford Mike J 
Published: September 2022

Abstract
Background: Anxiety about COVID-19 is common. For most people this is an appropriate response to the loss of livelihoods and loved-ones, disruptions to social networks, and uncertainty about the future. However, for others these anxieties relate to contracting the virus itself. Little is known about the characteristics of people with severe COVID anxiety or the impact it has on their daily lives.
Methods: We conducted a two-phase cross-sectional survey of people aged 18 or over who were living in United Kingdom, self-identified as anxious about COVID-19, and had a score of ≥9 on the Coronavirus Anxiety Scale. We recruited participants nationally through online adverts and locally via primary care services in London. Data on demographic and clinical factors were used in multiple regression modelling to examine the greatest contributors to functional impairment, poor health-related quality of life and protective behaviours in this sample of individuals with severe COVID anxiety.
Results: We recruited 306 people with severe COVID anxiety between January and September 2021. Most were female (n = 246, 81.2%); they had a median age of 40 (range = 18-83). The majority of participants also had generalised anxiety (n = 270, 91.5%), depression (n = 247, 85.5%), and a quarter (n = 79, 26.3%) reported a physical health condition which put them at increased risk of hospitalisation with COVID-19. Half had severe social dysfunction (n = 151, 52.4%). One in ten reported never leaving their home, one in three washed all items brought into their house, one in five washed their hands constantly, and one in five of those with children reported not sending them to school because of fears of COVID-19. Increasing co-morbid depressive symptoms best explained functional impairment and poor quality of life after controlling for other factors.
Conclusions: This study highlights the high degree of co-occuring mental health problems, and the extent of functional impairment and poor health-related quality of life among people with severe COVID anxiety. Further research is needed to establish the course of severe COVID anxiety as the pandemic progresses, and steps that can be taken to support people who experience this distress.
Nitration of Chemokine CXCL8 acts as a natural mechanism to limit acute inflammation
Authors: Thompson Sarah , Pang Chong Yun , Sepuru Krishna Mohan , Cambier Seppe , Hellyer Thomas P , Scott Jonathan , Simpson A. John , Proost Paul , Kirby John A. , Rajarathnam Krishna , Sheerin Neil S. , Ali Simi 
Published: September 2022

Abstract
Chemokine CXCL8 is a key facilitator of the human host immune response, mediating neutrophil migration and activation at the site of infection and injury. The oxidative burst is an important effector mechanism which leads to the generation of reactive nitrogen species (RNS), including peroxynitrite. The current study was performed to determine the potential for nitration to alter the biological properties of CXCL8 and its detection in human disease. Here, we show peroxynitrite nitrates CXCL8 and thereby regulates neutrophil migration and activation. The nitrated chemokine was unable to induce transendothelial neutrophil migration in vitro and failed to promote leukocyte recruitment in vivo. This reduced activity is due to impairment in both G protein-coupled receptor signaling and glycosaminoglycan binding. Using a novel antibody, nitrated CXCL8 was detected in broncheoalveolar lavage samples from patients with pneumonia. These findings were validated by mass spectrometry. Our results provide the first direct evidence of chemokine nitration in human pathophysiology and suggest a natural mechanism that limits acute inflammation.
Acalculous cholecystitis is a common extrahepatic manifestation of hepatitis E and suggests a more serious condition
Authors: Cao Xuemei , Jiang Wei , Shi Lingfeng , Wang Yanping , Chen Jie , Huang Wenxiang , Zhang Shujun 
Published: September 2022

Abstract
Background: The purpose of this study was to understand the incidence and clinical significance of acalculous cholecystitis in acute hepatitis E.
Patients and methods: A single center enrolled 114 patients with acute hepatitis E. All these patients had imaging of the gallbladder, and patients with gallstones and cholecystectomy were excluded.
Results: Acalculous cholecystitis was found in 66 patients (57.89%) with acute hepatitis E. The incidence of males was 63.95%, which was significantly higher than that of females (39.29%) (P=0.022). The mean hospital stays and the incidence of spontaneous peritonitis in patients with cholecystitis were significantly higher than those in patients without cholecystitis 20.12 ± 9.43 days and 9.09%, respectively, and significantly higher than those in patients without cholecystitis 12.98 ± 7.26 days and 0% (P<0.001 and P=0.032, respectively). ALB, TB, DB, CHE and PTA in patients with cholecystitis were significantly inferior to those without cholecystitis (P<0.001, P<0.001, P<0.001, P<0.001 and P= 0.003, respectively).
Conclusion: Acalculous cholecystitis is very common in patients with acute hepatitis E, and it may serve as a predictor of increased peritonitis, synthetic decompensation, and longer hospital stay with hepatitis E.
The Role of Multiple Sclerosis Therapies on the Dynamic of Human Gut Microbiota
Authors: Vacaras Vitalie , Muresanu Dafin F , Buzoianu Anca-Dana , Nistor Cristina , Vesa Stefan Cristian , Paraschiv Andreea Cristina , Botos-Vacaras Daniela , Vacaras Cristiana , Vithoulkas George 
Published: September 2022

Abstract
Gut microbiota, the total microorganisms in our gastrointestinal tract, might have an implication in multiple sclerosis (MS), a demyelinating neurological disease. Our study included 50 MS patients and 21 healthy controls (HC). Twenty patients received a disease modifying therapy (DMT), interferon beta1a or teriflunomide, 19 DMT combined with homeopathy and 11 patients accepted only homeopathy. We collected in total 142 gut samples, two for each individual: at the study enrolment and eight weeks after treatment. We compared MS patients’ microbiome with HC, we analysed its evolution in time and the effect of interferon beta1a, teriflunomide and homeopathy. There was no difference in alpha diversity, only two beta diversity results related to homeopathy. Compared to HC, untreated MS patients had a decrease of Actinobacteria, Bifidobacterium, Faecalibacterium prauznitzii and increased Prevotella stercorea, while treated patients presented lowered Ruminococcus and Clostridium. Compared to the initial sample, treated MS patients had a decrease of Lachnospiraceae and Ruminococcus and an increased Enterococcus faecalis. Eubacterium oxidoreducens was reduced after homeopathic treatment. The study revealed that MS patients may present dysbiosis. Treatment with interferon beta1a, teriflunomide or homeopathy implied several taxonomic changes. DMTs and homeopathy might influence the gut microbiota.
Partnership and domestic resource mobilization in Sudan for cVDPV2 outbreak response amidst multiple emergencies in 2020-2021
Authors: Mashal Mohammed Taufiq , Eltayeb Dalya , Higgins-Steele Ariel , Sheikh Ismael Suleiman El , Saeed Abid Ni’ma , Shukla Hemant , Machado Leonard 
Published: September 2022

Abstract
Background: During 2020 and immediately prior to the COVID-19 pandemic, Sudan was experiencing multiple emergencies including violence, seasonal flooding, and vector-borne disease outbreaks. After more than ten years since its last case of wild poliovirus, Sudan declared a circulating vaccine-derived poliovirus type 2 (cVDPV2) outbreak on 9 August 2020.
Methods: cVDPV2 outbreak response data and programme documents of the Federal Ministry of Health and WHO were reviewed. Surveillance data was verified through WHO-recommended procedures for detecting and characterizing polioviruses from stool and sewage samples collected from AFP cases and the environment.
Results: This outbreak in Sudan led to a total of 58 confirmed cases of cVDPV2 from 15 of the 18 states. Two nationwide vaccination campaigns were held to increase immunity of children under-five against poliovirus type 2. Funding challenges were overcome by intense additional resource mobilization from domestic resources and partnerships. The funding gap was bridged from domestic resources (49%) sourced through GPEI partners, and in-country humanitarian funding mechanisms.
Conclusions: During an outbreak response and challenge of funding shortfall, mobilizing domestic resources is possible through coordinated approaches and systematic communication with in-country partners, disaggregation of needs, and diverse interventions. A cVDPV2 outbreak requires a fast, resourced, and quality response to stop virus circulation.
Simulation studies on line, light and human following robots using Arduino in Proteus Schematic
Authors: Jebaselvi G. D. Anbarasi , Narmadha R. , Anand Vishwajeet , Tathagat Harshit 
Published: September 2022

Abstract
This technical paper describes about the robots which follows the path, light and human gestures and their simulation using Arduino and Proteus. The line following robot is constructed to proceed in a predefined path using IR sensor. The light following robot is designed to follow the light source LDR, the light dependent resistor. The human following robot traces the human heat and moves in the direction of human motion or gesture by means of IR, the infra-red sensor. Their applications involve in the fields of remote sensing, mobile communications and biomedical instrumentation and so on. The key merits include its’ lightweight, automated, cheap, easy to use, etc. Here the Proteus schematic has been drawn and its simulation uses Arduino software.
Optical and electronic properties enhancement via chalcogenides: Promising materials for DSSC applications
Authors: Deogratias Geradius , Al−Qurashi Ohoud S. , Wazzan Nuha 
Published: September 2022

Abstract
Comparatively, sensitizers featuring the chalcogen family are less heavily investigated despite their known electronic properties in metal-based materials. In this work, an array of optoelectronic properties is reported using quantum chemical methods. Red-shifted bands within the UV − vis absorption spectrum with absorption maxima > 500 nm in the order of increasing chalcogenides atomic size were observed. There is a monotonic down-shift in the LUMO and ESOP energy consistent with atomic orbital energies (p orbitals) which increases down the group O 2p, S 3p, Se 4p toTe 5p. The excited-state lifetime and free energies of charge injection follow the decreasing order of chalcogenides electronegativity. Adsorption energies of dyes on TiO2 anatase (101) are in the range of − 0.08 to − 0.77 eV. Based on evaluated properties, selenium and tellurium-based materials hold promise for their futuristic device applications; therefore, this work motivates continued investigation of the chalcogenides sensitizers and their application.
The invariant ion-acoustic waves in the plasma
Authors: Saberian Ehsan 
Published: September 2022

Abstract
We have formulated the invariant ion-acoustic waves (IAWs) in the astrophysical plasmas including the pure thermodynamic features of the background particles. Here, we have used the modern version of the kappa distribution formalism, where it is labeled with an invariant kappa index as of the zero dimensionality spectral index, κ0. Two contexts for studying the invariant IAWs have been employed, i.e., the kinetic theory formalism and the hydrodynamic fluid description. At first, we have employed the Vlasov-Poisson equations at the low frequency band of the weakly damped ion waves, where the most generalized formalism of the ion-sound speed has been confirmed in terms of the extended polytropic indices of the plasma species, γj. Furthermore, the Landau damping of IAWs has been formulated in terms of κ0, the wavelength, and the temperatures of the plasma species. In the hydrodynamic description, we have normalized the fluid parameters by using the extended quantities, including the generalized ion-sound speed and Debye length. Then, by using the perturbation theory in the linear and nonlinear regimes, some missing issues in the formulation of the invariant IAWs have been emphasized, such as the effect of the perturbed potential degrees of freedom, and the combined effects of the wave steepening and dispersion. The second feature has been analyzed by deriving a generalized KdV equation and its solitary wave solutions in an invariant formalism. Here, farther/near-equilibrium regions, corresponding to the thermodynamic evolutions, have been characterized by 0 < κ0 < 1 (0 < γj < 0.5) and κ0 > 1 (0.5 < γj < 1), respectively, where we have analyzed the IAWs in the anti-equilibrium state at κ0 → 0 (γj → 0) towards the equilibrium state at κ0 ≫ 1 (γj → 1). Our numerical analysis confirms the distinction between the IAWs diagrams in two regions, where the transition from far-equilibrium states to the near-equilibrium states occurs in the vicinity κ0 ∼ 1 (γj ∼ 0.5), denoting the escape state of the evolution.