bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative Folate Receptor-Positive Circulating Tumor Cells Are Associated With Occult Peritoneal Metastasis and Early Recurrence in Gastric Cancer Patients: A Prospective Cohort Study
Authors: Zeng Ci Dian Dan , Jin Cheng Cheng , Gao Chun , Xiao Ai Tang , Tong Yi Xin , Zhang Sheng 
Published: March 2022

BackgroundThe aim of this study is to explore the clinical feasibility of detecting folate receptor-positive circulating tumor cells (FR+ CTCs) for predicting peritoneal metastasis and short-term outcome in gastric cancer patients.MethodsThis is a prospective, single-center, observational study. We applied ligand-targeted enzyme-linked polymerization method to detect preoperative FR+ CTC levels in peripheral blood. We evaluated the diagnostic value of FR+ CTCs and other biomarkers in predicting peritoneal metastasis. Prognostic factors for recurrence-free survival (RFS) were investigated in univariate and multivariate analyses.ResultsA total of 132 patients with gastric cancer and 9 patients with benign disease were recruited. Gastric cancer patients had a significantly higher CTC level compared to that of patients with benign disease (p &lt; 0.01). Combined model including CTC level and other biomarkers presented high sensitivity (100%) and moderate specificity (59.3%) in predicting peritoneal metastasis. Univariate analysis revealed that decreased serum prealbumin, decreased peripheral lymphocyte count, FR+ CTCs, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and lymph node metastasis were significantly associated with shorter RFS. FR+ CTC level [≥12.6 folate units (FU)/3 ml, hazard ratio (HR) = 6.957, p = 0.005] and CA19-9 (&gt;34 ng/ml, HR = 3.855, p = 0.037) were independent prognostic factors in multivariate analysis.ConclusionsOur findings for the first time suggested the diagnostic value of preoperative CTC levels in predicting peritoneal metastasis in gastric cancer. Moreover, the FR+ CTC level could be a novel and promising prognostic factor for the recurrence of gastric cancer in patients who underwent surgery.Clinical Trial RegistrationChinese Clinic Trial Registry, identifier ChiCTR2100050514.
Identification of LncRNA Prognostic Signature Associated With Genomic Instability in Pancreatic Adenocarcinoma
Authors: Zhu Jinfeng , Huang Qian , Peng Xingyu , Luo Chen , Liu Sicheng , Liu Zitao , Wu Xun , Luo Hongliang 
Published: March 2022

BackgroundGenomic instability (GI) is a critical feature of cancer which plays a key role in the occurrence and development of pancreatic adenocarcinoma (PAAD). Long non-coding RNA (LncRNA) is an emerging prognostic biomarker because it is involved in regulating GI. Recently, researchers used such GI-related LncRNAs (GILncRNAs) to establish a prognostic signature for patients with cancer and helped in predicting the overall prognosis of the patients. However, it is evident that patients with PAAD still lack such prognostic signature constructed with GILncRNA.MethodsThe present study screened GILncRNAs from 83 patients with PAAD. Prognosis-related GILncRNAs were identified by univariate Cox regression analysis. The correlation coefficients of these GILncRNAs were obtained by multivariate Cox regression analysis and used to construct a signature. The signature in the present study was then assessed through survival analysis, mutation correlation analysis, independent prognostic analysis, and clinical stratification analysis in the training set and validated in the testing as well as all TCGA set. The current study performed external clinical relevance validation of the signature and validated the effect of AC108134.2 in GILncSig on PAAD using in vitro experiments. Finally, the function of GILncRNA signature (GILncSig) dependent on Gene Ontology enrichment analysis was explored and chemotherapeutic drug sensitivity analysis was also performed.ResultsResults of the present study found that a total of 409 GILncRNAs were identified, 5 of which constituted the prognostic risk signature in this study, namely, AC095057.3, AC108134.2, AC124798.1, AL606834.1, and AC104695.4. It was found that the signature of the present study was better than others in predicting the overall survival and applied to patients with PAAD of all ages, genders, and tumor grades. Further, it was noted that the signature of the current study in the GSE102238, was correlated with tumor length, and tumor stage of patients with PAAD. In vitro, functional experiments were used in the present study to validate that AC108134.2 is associated with PAAD genomic instability and progression. Notably, results of the pRRophetic analysis in the current study showed that the high-risk group possessed reverse characteristics and was sensitive to chemotherapy.ConclusionsIn conclusion, it was evident that the GILncSig used in the present study has good prognostic performance. Therefore, the signature may become a potential sensitive biological indicator of PAAD chemotherapy, which may help in clinical decision-making and management of patients with cancer.
Quantitative Dynamic-Enhanced MRI and Intravoxel Incoherent Motion Diffusion−Weighted Imaging for Prediction of the Pathological Response to Neoadjuvant Chemotherapy and the Prognosis in Locally Advanced Gastric Cancer
Authors: Zhu Yongjian , Jiang Zhichao , Wang Bingzhi , Li Ying , Jiang Jun , Zhong Yuxin , Wang Sicong , Jiang Liming 
Published: March 2022

BackgroundThis study aimed to explore the predictive value of quantitative dynamic contrast-enhanced MRI (DCE-MRI) and intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) quantitative parameters for the response to neoadjuvant chemotherapy (NCT) in locally advanced gastric cancer (LAGC) patients, and the relationship between the prediction results and patients’ prognosis, so as to provide a basis for clinical individualized precision treatment.MethodsOne hundred twenty-nine newly diagnosed LAGC patients who underwent IVIM-DWI and DCE-MRI pretreatment were enrolled in this study. Pathological tumor regression grade (TRG) served as the reference standard of NCT response evaluation. The differences in DCE-MRI and IVIM-DWI parameters between pathological responders (pR) and pathological non-responders (pNR) groups were analyzed. Univariate and multivariate logistic regressions were used to identify independent predictive parameters for NCT response. Prediction models were built with statistically significant quantitative parameters and their combinations. The performance of these quantitative parameters and models was evaluated using receiver operating characteristic (ROC) analysis. Clinicopathological variables, DCE-MRI and IVIM-DWI derived parameters, as well as the prediction model were analyzed in relation to 2-year recurrence-free survival (RFS) by using Cox proportional hazards model. RFS was compared using the Kaplan–Meier method and the log-rank test.ResultsSixty-nine patients were classified as pR and 60 were pNR. Ktrans, kep, and ve values in the pR group were significantly higher, while ADCstandard and D values were significantly lower than those in the pNR group. Multivariate logistic regression analysis demonstrated that Ktrans, kep, ve, and D values were independent predictors for NCT response. The combined predictive model, which consisted of DCE-MRI and IVIM-DWI, showed the best prediction performance with an area under the curve (AUC) of 0.922. Multivariate Cox regression analysis showed that ypStage III and NCT response predicted by the IVIM-DWI model were independent predictors of poor RFS. The IVIM-DWI model could significantly stratify median RFS (52 vs. 15 months) and 2-year RFS rate (72.3% vs. 21.8%) of LAGC.ConclusionPretreatment DCE-MRI quantitative parameters Ktrans, kep, ve, and IVIM-DWI parameter D value were independent predictors of NCT response for LAGC patients. The regression model based on baseline DCE-MRI, IVIM-DWI, and their combination could help RFS stratification of LAGC patients.
Establishment of Cutoff Values for Newborn Screening of Six Lysosomal Storage Disorders by Tandem Mass Spectrometry
Authors: Li Ruotong , Tian Liping , Gao Qing , Guo Yuanfang , Li Gaijie , Li Yulin , Sun Meng , Yan Yan , Li Qing , Nie Wenying , Zou Hui 
Published: March 2022

ObjectiveLysosomal storage disorders (LSDs) are becoming increasingly important in newborn screening, and tandem mass spectrometry (MS/MS) is widely used in newborn screening for LSDs through measurement of enzymatic activities in dried blood spots (DBSs). Overall, the determination of the cutoff value is important in such screening, and different laboratories have different methods of determining this value; most do not use a fixed cutoff value but rather calculate the corresponding batch cutoff value based on each batch of experimental data. In this study, we used MS/MS to screen for LSDs and sought to find an appropriate method to establish the cutoff value for LSD screening.MethodsA total of 38,945 samples from newborn blood tablets collected from various maternity hospitals in six cities in Shandong province, including Jinan, Dezhou, Heze, Linyi, Weifang, and Zibo, were tested using a Waters Xevo TQD tandem mass spectrometer; the experimental data were analyzed with MassLynx V4.1. The laboratory used 30% of the median GLA enzyme activity and 20% of the median ABG, ASM, GALC, IDUA, and GAA enzyme activities in every test as the cutoff values for that batch of experiments.ResultsThere were 254 suspicious positives in the initial screening test, including one case of Gaucher disease, one of Niemann-Pick disease, 47 of Krabbe disease, four of MPS-I, 21 of Fabry disease, and 180 of Pompe disease. After genetic screening, 11 children were diagnosed, including three with Pompe disease, three with Fabry disease, and five with Krabbe disease. In addition, the enzyme activity cutoff value of this experiment showed seasonal variation, which was initially believed to be related to the ambient temperature, such as the effect of ambient temperature on the human body or the temperature when the blood tablets dried naturally.ConclusionOverall, MS/MS can be used in LSD screening, and using different cutoff values in each batch of experiments is feasible. The ambient temperature might be a reason why the enzyme activity cutoff value has seasonal variation. More samples are needed to develop a method of determining cutoff values in laboratories.
Design of a Participatory Organizational-Level Work Stress Prevention Approach in Primary Education
Authors: Bakhuys Roozeboom Maartje C. , Niks Irene M. W. , Schelvis Roosmarijn M. C. , Wiezer Noortje M. , Boot Cécile R. L. 
Published: March 2022

BackgroundWork stress is a serious problem in primary education. Decades of research underline the importance of participatory, organizational-level work stress prevention approaches. In this approach, measures are planned to tackle causes of work stress in a participatory manner and implemented by a working group consisting of members of the organization. This approach can only be effective if the measures contain effective ingredients to decrease work stress risks and are successfully implemented. The aim of this paper is to present an outline of a work stress prevention approach that is evaluated in primary education. To ensure the appropriateness of measures, a logic model of change is built as part of the risk assessment to facilitate the selection of appropriate measures. Progression on target behaviors as well as implementation factors are real-time monitored during implementation and fed back to the working groups, to provide the opportunity to adjust action plans when needed to optimize implementation.MethodsThe approach consists of five steps: (1) preparation: installing an advisory board and working groups, (2) risk assessment: inventory of work stress risks (questionnaires and focus groups). In addition, a behavioral analysis is performed to build a logic model of change to facilitate selection of measures, (3) action planning: conducting an action plan with appropriate measures (focus groups), (4) implementation: implementing the action plan. During implementation progression on target behaviors and implementation factors are monthly monitored and fed back to the working groups, and (5) evaluation: effects of the approach are studied in a controlled trial with measurements at baseline (T0), 1 year (T1), and 2 years (T2) follow-up. A process evaluation is carried out using quantitative (questionnaires and real-time monitoring data) and qualitative (interviews and data logs) data to study the implementation process of all steps of the work stress approach.DiscussionWe believe that building a logic model of change and real-time monitoring of implementation could be of added value to improve the success of the work stress prevention approach. With this study, we aim to provide more insights into work stress intervention research, especially in primary education.Clinical Trial RegistrationThe study is registered in Netherlands Trial Register (ClinicalTrials.gov #NL9797, October 18, 2021).
The Effects of Online Mindfulness-Based Intervention on Posttraumatic Stress Disorder and Complex Posttraumatic Stress Disorder Symptoms: A Randomized Controlled Trial With 3-Month Follow-Up
Authors: Dumarkaite Austeja , Truskauskaite-Kuneviciene Inga , Andersson Gerhard , Kazlauskas Evaldas 
Published: March 2022

ObjectivesMindfulness-based interventions have recently been shown to be a promising option for treating posttraumatic stress. The current study aimed to investigate the effects of an online mindfulness-based intervention on ICD-11 posttraumatic stress disorder (PTSD) and complex PTSD (CPTSD) symptoms at a 3-month follow-up.MethodsAn RCT design with three measurement points (pre-intervention, post-intervention, and 3-month follow-up) was used to investigate the effects of an 8-week online mindfulness intervention. In total, 53 traumatized young adults (Mage = 23.21, SDage = 2.81; 84.9% female) participated in the study: 17 in the intervention group and 36 in the waiting list control group.ResultsIntervention group and waiting list control group comparison revealed that the intervention was effective for reducing CPTSD disturbances in self-organization symptoms (d = −0.84 [−1.44; −0.24]), specifically, negative self-concept (d = −0.66 [−1.25; −0.07]) and disturbances in relationships (d = −0.87 [−1.47; −0.27]), at 3-month follow-up. There were no between-group effects for PTSD symptoms from pre-test to follow-up.ConclusionThis is one of the first RCT studies to report follow-up effects of an online mindfulness-based intervention for ICD-11 PTSD or CPTSD symptoms. Our study yielded that the effects of mindfulness-based internet intervention on CPTSD symptoms tend to retain over time.Trial RegistrationThis study was registered with ClinicalTrials.gov (NCT number: NCT04333667; https://clinicaltrials.gov/ct2/show/NCT04333667). Registered April 3, 2020.
Health Status and Association With Interpersonal Relationships Among Chinese Children From Urban Migrant to Rural Left-Behind
Authors: Zhao Guanlan , Wang Menmen , Xu Jiayao , Lu Jingjing , Akezhuoli Hailati , Wang Feng 
Published: March 2022

BackgroundTo date, numerous studies have examined the health status of Chinese left-behind children and migrant children. However, the impact of children's diverse migration/left-behind experiences on their health is still unclear.MethodsA cross-sectional survey was conducted in 2020 in Nanling country (Anhui province) and Kaihua country (Zhejiang province) in China. School children from grade 5 to 8 reported their socio-demographic, interpersonal relationships, self-rated health, suicidal ideation, and depression. Participants were divided into four groups based on their migrant patterns, namely rural left-behind children with previous migration experience (ME-LBC), rural children with previous migration experience (ME-NLBC), rural left-behind children without migration experience (LBC), and rural children without migration experience (NLBC).ResultsAmong 2,323 participants included in the present study, there were 336 ME-LBC (14.5%), 283 ME-NLBC (12.2%), 561 LBC (24.1%) and 1,143 NLBC (49.2%). Compared with NLBC, ME-LBC reported significantly poorer self-rated health (OR = 0.72, 95% CI [0.53–0.97], p &lt; 0.05), higher risk of depression (β = 0.90, 95% CI [0.02–1.77], p &lt; 0.05) with adjustment of socio-demographic and interpersonal relationships. There was no significant difference in suicidal ideation among different groups of children. The better interpersonal relationship was associated with a better self-rated health, and lower prevalence of depression and suicidal intention.ConclusionsCompared to ordinary rural children, ME-LBC tended to experience higher levels of depression and poorer self-rated health. These research findings imply developing intervention programs about psychological adjustment tailored to different migrant patterns of Chinese rural children. The keys might be to strengthen the relationships with peer and teacher in school and improve the quality of parent-child communication in family for LBC.
FORMATION OF CHILDREN’S CREATIVITY DUE TO THE UPDATED BASIC COMPONENT OF PRESCHOOL EDUCATION (2021)
Authors: HNIZDILOVA O. , SULAIEVA N. 
Published: March 2022

The article is devoted to the study of preschool children’s creativity. It is revealed that, at the present stage of psychology and pedagogy development, the concept of creativity expands to infinity and sometimes has a vague character. The author finds that creativity is an essential integral personal ability that is displayed throughout a person’s life path. It is intensified in all spheres of life, namely political, economic, social, and spiritual. Creativity helps a person to self-model and begins to form in preschool age in various types of children’s activities. It has seven creative parameters, in particular, motivational, emotional, intellectual, aesthetic, existential, communicative, and competence. Awareness of the essence of the concept of creativity and its parameters will help educational practice.
The author analyzes and clarifies whether the objective of forming a child’s creative personality exists in modern normative-methodical documents of preschool education of Ukraine. It has been found that there is no direct description and separate methodological recommendations for the formation of this key personal quality in the updated state standard of preschool education. But it has been found that indicators of preschoolers’ creativity and guidelines for their formation permeate almost all educational areas in the Basic component of preschool education (2021). They are components of almost all competencies’ formation. It confirms the integrated nature of this personal phenomenon, and pedagogical tools have the potential for its formation. The semantic and procedural components of preschool children’s creativity formation identified by the author will help preschool educators to have a clear ‘portrait of a creative preschooler’ and work effectively in the direction of this educational objective.
PRESCHOOL CHILDREN’S SENSITIVITY FORMATION BY MEANS OF THEATRICAL GAMES
Authors: SULAIEVA N. 
Published: March 2022

The article highlights the issue of preschool children’s sensitivity formation by means of theatrical games. Emphasis is placed on the signs of this quality, and it is determined that preschool age is a sensitive period for its formation.
It is noted that theatrical and play activities are a priority type of the life experience display and a leading means of preschool children’s moral education. The range of types of theatrical games is presented, and the possibilities of their introduction into the educational process of preschool educational institutions are characterized.
Emphasis is placed on the value of involving preschoolers in theatrical play activities as spectators and direct participants in the action, in particular, as actors, musicians, choreographers, decorators, illuminators, etc.
The stages of preparatory work for preschoolers’ theatrical and play activities are determined. The pedagogical potential of theatrical games based on the content of literary works for sensitivity formation as a preschool child’s key quality is revealed.
EDUCATIONAL AND SCIENTIFIC LABORATORY OF INNOVATIVE EDUCATIONAL SOLUTIONS IN PRIMARY EDUCATION: PEDAGOGICAL TRENDS AND CREATIVE IDEAS
Authors: FEDIY O. , PROTSAI L. , GIBALOVA N. , POCHYNOK Y. 
Published: March 2022

The experience of educational and scientific laboratory of Innovative educational solutions developed on the chair of primary education, natural and mathematical disciplines and teaching methods of PNPU named after V. G. Korolenko has been generalized and its brief haracteristic activity has been presented. The basic principles of the laboratory functionality which determine its place and role in the system of training specialists in the field of primary education have been distinguished. Emphasis is placed on the fact that the laboratory is an open platform for creating the preconditions for an effective combination of education, science and innovation. It has been pointed out that the laboratory environment meets the modern requirements of the new Ukrainian school regarding the organization of the educational space for junior students. The model variants and their use methods of the primary classroom hubs equipping built on the principles of pedagogical ergodesign have been illustrated.
Clinical Case of Mitral Valve Prosthesis Dysfunction Due to Impaired Anticoagulant Therapy in a Patient with High-Risk Coronary Heart Disease
Authors: Гогаева Е.К. , Лазоришинец В.В. , Руденко А.В. , Дзахоева Л.С. , Крикунов А.А. 
Published: March 2022

Успешное проведение кардиохирургического вмешательства является основным звеном лечения пациента с ИБС высокого риска. Однако не менее важным аспектом хорошего результата оперативного вмешательства и улучшения качества жизни пациентов является качественное кардиологическое сопровождение на всех этапах пребывания пациента в стационаре и после выписки. В данной статье представлен клинический случай пациента высокого риска, которому были успешно выполнены операция резекции заднебоковой аневризмы левого желудочка (ЛЖ), протезирование митрального клапана, повторная госпитализация пациента в институт обусловлена дисфункцией протеза вследствие нарушения приема антикоагулянтной терапии после кровотечения из язвы луковицы двенадцатиперстной кишки. При невозможности выполнить реоперацию и провести тромболитическую терапию пациенту после ЖКК единственной альтернативой является адекватная антикоагулянтная терапия с целевым МНО 3,0, что позволило нам выписать пациента без дисфункции протеза в удовлетворительном состоянии.
Successful cardiac surgery is a key part of treatment of patient with high-risk coronary artery disease. However, an equally important aspect of the good result of surgical intervention and improvement of the quality of life of patient is high-quality cardiological support at all stages of hospital stay and after discharge. In this article, we describe the clinical case of high-risk patient, for who we performed resection of posterior lateral aneurysm of the left ventricle (LV) and mitral valve repair. Repeated hospitalization at the Institute is caused by prosthetic mitral valve dysfunction due to impaired anticoagulant therapy after bleeding from a duodenal ulcer. In case of inabilityto perform reoperation and thrombolytic therapy for patient after bleeding, the only alternative is adequate anticoagulant therapy with a target INR 3.0 that normalized the function of the mitral valve prosthesis and let to discharge the patient in a satisfactory condition.