bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of the COVID-19 pandemic on racial and ethnic minorities in Japan
Authors: Nomoto Hidetoshi , Asai Yusuke , Hayakawa Kayoko , Matsunaga Nobuaki , Kutsuna Satoshi , Kodama Eiichi N. , Ohmagari Norio 
Published: October 2022

Abstract
This study compared clinico-epidemiological characteristics between Japanese and non-Japanese coronavirus disease 2019 (COVID-19) patients under the pandemic in Japan. We retrospectively analysed nationwide data of hospitalised COVID-19 patients before 31 March 2021. Epidemic curves were constructed to identify the case distribution over time. A total of 28 093 patients were Japanese and 1335 patients were non-Japanese. The major racial and ethnic minorities were East Asians (n = 521), South Asians (n = 260) and Latin Americans (n = 270). Non-Japanese patients were younger and more likely to travel to COVID-19 endemic countries (7.7%), had meals with other people (17.8%), stayed in crowded places (17.9%) and worked mainly in restaurants (6.6%) and service facilities in nightlife businesses (5.2%). In the matched cohorts, we found no clear disparities in time to admission and clinical prognoses. The epidemic curve for non-Japanese patients showed a small peak in the first wave and no definite waves for the second or third waves. Racial and ethnic minorities were at less risk of severe disease but were at a greater risk of COVID-19 exposure; however, the healthcare system in Japan may provide them with equal opportunities to access inpatient care with Japanese. Further research on their social determinants of health in Japan is required.
The Manchukuo Young Girl Envoys and their visit to Japan: an underestimated prelude of Japan–Manchukuo interactions, June 22–July 12, 1932
Authors: Yuan Jianda 
Published: October 2022

AbstractThis study analyzes the little known Manchukuo Young Girl Envoys – Manchukuo's first government-appointed diplomats – and their official visit to Japan between June 22 and July 12, 1932. Existing studies on the Envoys tend to interpret them from an angle of contemporary Japanese people's national sentiment and deem their visit to Japan a show that the Japanese authorities in Manchukuo and Japan orchestrated together, to render Japan a strong world power. This study problematizes that view and considers the Envoys more of a product of intense power struggles inside Manchukuo's highest Japanese ruling strata, suggesting that Manchukuo's decision-making circle in 1932 was far from being a unified entity. Examining the Envoys' interactions in Japan and comparing relevant Japanese- and Chinese-language news coverage on them, this study argues for the possibility of tracing the intertwined national ideals of Manchukuo's Chinese and Japanese government leaders based on the inspiring example of the Envoys.
Taming Violence: The Shanghai Green Gang and its Self-Legitimation Claims in the Early Twentieth Century
Authors: Luo Wei 
Published: October 2022

AbstractFrom the end of the nineteenth century to the mid-twentieth century, gangs and secret societies (banghui) exercised enormous influence on Chinese society and politics. The two portrayals ofbanghuiin historiography – as modern criminal syndicates that came out of an emerging capitalist economy or, alternatively, a cultural succession of traditional secret society – are so distinct that their proponents often speak past each other. Revisiting primary and secondary materials on the Shanghai Green Gang, one of the most activebanghuiorganizations during the Republican era (1911–49), this article aims to bridge the two understandings by focusing on the gang’s self-legitimation claims. Facing rapid social changes of the early twentieth century, I argue, the Green Gang reframed its use of violence as disciplinary, revolutionary, and nationalistic to gain public legitimation. Together with its involvement in state building and resource extraction, the gang’s cultural work contributed to its prominence and led multiple political authorities to promote it to an exceptional degree.
Beyond the Ångström Exponent: Probing Additional Information in Spectral Curvature and Variability of In Situ Aerosol Hyperspectral (0.3–0.7 μm) Optical Properties
Authors: Jordan Carolyn E. , Anderson Bruce E. , Barrick John D. , Blum Dani , Brunke Kathleen , Chai Jiajue , Chen Gao , Crosbie Ewan C. , Dibb Jack E. , Dillner Ann M. , Gargulinski Emily , Hudgins Charles H. , Joyce Emily , Kaspari Jackson , Martin Robert F. , Moore Richard H. , O’Brien Rachel , Robinson Claire E. , Schuster Gregory L. , Shingler Taylor J. , Shook Michael A. , Soja Amber J. , Thornhill Kenneth L. , Weakley Andrew T. , Wiggins Elizabeth B. , Winstead Edward L. , Ziemba Luke D. 
Published: October 2022

AbstractÅngström exponents (α) allow reconstruction of aerosol optical spectra over a broad range of wavelengths from measurements at two or more wavelengths. Hyperspectral measurements of atmospheric aerosols provide opportunities to probe measured spectra for information inaccessible from only a few wavelengths. Four sets of hyperspectral in situ aerosol optical coefficients (aerosol‐phase total extinction, σext, and absorption, σabs; liquid‐phase soluble absorption from methanol, σMeOH‐abs, and water, σDI‐abs, extracts) were measured from biomass burning aerosols (BBAs). Hyperspectral single scattering albedo (ω), calculated from σext and σabs, provide spectral resolution over a wide spectral range rare for this optical parameter. Observed spectral shifts between σabs and σMeOH‐abs/σDI‐abs argue in favor of measuring σabs rather than reconstructing it from liquid extracts. Logarithmically transformed spectra exhibited curvature better fit by second‐order polynomials than linear α. Mapping second order fit coefficients (a1, a2) revealed samples from a given fire tended to cluster together, that is, aerosol spectra from a given fire were similar to each other and somewhat distinct from others. Separation in (a1, a2) space for spectra with the same α suggest additional information in second‐order parameterization absent from the linear fit. Spectral features found in the fit residuals indicate more information in the measured spectra than captured by the fits. Above‐detection σMeOH‐abs at 0.7 μm suggests assuming all absorption at long visible wavelengths is BC to partition absorption between BC and brown carbon (BrC) overestimates BC and underestimates BrC across the spectral range. Hyperspectral measurements may eventually discriminate BBA among fires in different ecosystems under variable conditions.
Dominance of phage particles carrying antibiotic resistance genes in the viromes of retail food sources
Authors: Blanco-Picazo Pedro , Morales-Cortes Sara , Ramos-Barbero María Dolores , García-Aljaro Cristina , Rodríguez-Rubio Lorena , Muniesa Maite 
Published: October 2022

Abstract
The growth of antibiotic resistance has stimulated interest in understanding the mechanisms by which antibiotic resistance genes (ARG) are mobilized. Among them, studies analyzing the presence of ARGs in the viral fraction of environmental, food and human samples, and reporting bacteriophages as vehicles of ARG transmission, have been the focus of increasing research. However, it has been argued that in these studies the abundance of phages carrying ARGs has been overestimated due to experimental contamination with non-packaged bacterial DNA or other elements such as outer membrane vesicles (OMVs). This study aims to shed light on the extent to which phages, OMVs or contaminating non-packaged DNA contribute as carriers of ARGs in the viromes. The viral fractions of three types of food (chicken, fish, and mussels) were selected as sources of ARG-carrying phage particles, whose ability to infect and propagate in an Escherichia coli host was confirmed after isolation. The ARG-containing fraction was further purified by CsCl density gradient centrifugation and, after removal of DNA outside the capsids, ARGs inside the particles were confirmed. The purified fraction was stained with SYBR Gold, which allowed the visualization of phage capsids attached to and infecting E. coli cells. Phages with Myoviridae and Siphoviridae morphology were observed by electron microscopy. The proteins in the purified fraction belonged predominantly to phages (71.8% in fish, 52.9% in mussels, 78.7% in chicken sample 1, and 64.1% in chicken sample 2), mainly corresponding to tail, capsid, and other structural proteins, whereas membrane proteins, expected to be abundant if OMVs were present, accounted for only 3.8–21.4% of the protein content. The predominance of phage particles in the viromes supports the reliability of the protocols used in this study and in recent findings on the abundance of ARG-carrying phage particles.
TAB182 aggravates progression of esophageal squamous cell carcinoma by enhancing β-catenin nuclear translocation through FHL2 dependent manner
Authors: Gao Aidi , Su Zhenzi , Shang Zengfu , He Chao , Miao Dongliu , Li Xiaoqing , Zou Shitao , Ding Weiqun , Zhou Yue , Sun Ming , Zhou Jundong 
Published: October 2022

AbstractTAB182 (also named TNKS1BP1), a binding protein of tankyrase 1, has been found to participate in DNA repair. Our previous study has revealed the involvement of TAB182 in the radioresistance of esophageal squamous cell carcinoma (ESCC) cells. However, whether TAB182 contributes to the ESCC tumorigenesis and progression remains unclear. In this study, we found that highly expressed TAB182 is closely associated with a poor prognosis of patients with ESCC. TAB182 silencing reduced ESCC cell proliferation and invasion in vitro, tumorigenicity and metastasis in vivo. RNA-seq and IP-MS analysis revealed that TAB182 could affect the β-catenin signaling pathway via interacting with β-catenin. Furthermore, TAB182 prevented β-catenin to be phosphorylated by GSK3β and recruited four and a half of LIM-only protein 2 (FHL2), which thereby promoted β-catenin nucleus translocation to result in activation of the downstream targets transcription in ESCC cells. Our findings demonstrate that TAB182 enhances tumorigenesis of esophageal cancer by promoting the activation of the β-catenin signaling pathway, which provides new insights into the molecular mechanisms by which TAB182 accelerates progression of ESCC.
Kynurenine promotes neonatal heart regeneration by stimulating cardiomyocyte proliferation and cardiac angiogenesis
Authors: Zhang Donghong , Ning Jinfeng , Ramprasath Tharmarajan , Yu Changjiang , Zheng Xiaoxu , Song Ping , Xie Zhonglin , Zou Ming-Hui 
Published: October 2022

AbstractIndoleamine 2,3 dioxygenase-1 (IDO1) catalyzes tryptophan-kynurenine metabolism in many inflammatory and cancer diseases. Of note, acute inflammation that occurs immediately after heart injury is essential for neonatal cardiomyocyte proliferation and heart regeneration. However, the IDO1-catalyzed tryptophan metabolism during heart regeneration is largely unexplored. Here, we find that apical neonatal mouse heart resection surgery led to rapid and consistent increases in cardiac IDO1 expression and kynurenine accumulation. Cardiac deletion of Ido1 gene or chemical inhibition of IDO1 impairs heart regeneration. Mechanistically, elevated kynurenine triggers cardiomyocyte proliferation by activating the cytoplasmic aryl hydrocarbon receptor-SRC-YAP/ERK pathway. In addition, cardiomyocyte-derived kynurenine transports to endothelial cells and stimulates cardiac angiogenesis by promoting aryl hydrocarbon receptor nuclear translocation and enhancing vascular endothelial growth factor A expression. Notably, Ahr deletion prevents indoleamine 2,3 dioxygenase -kynurenine–associated heart regeneration. In summary, increasing indoleamine 2,3 dioxygenase-derived kynurenine level promotes cardiac regeneration by functioning as an endogenous regulator of cardiomyocyte proliferation and cardiac angiogenesis.
Decreased susceptibility of Plasmodium falciparum to both dihydroartemisinin and lumefantrine in northern Uganda
Authors: Tumwebaze Patrick K. , Conrad Melissa D. , Okitwi Martin , Orena Stephen , Byaruhanga Oswald , Katairo Thomas , Legac Jennifer , Garg Shreeya , Giesbrecht David , Smith Sawyer R. , Ceja Frida G. , Nsobya Samuel L. , Bailey Jeffrey A. , Cooper Roland A. , Rosenthal Philip J. 
Published: October 2022

AbstractArtemisinin partial resistance may facilitate selection of Plasmodium falciparum resistant to combination therapy partner drugs. We evaluated 99 P. falciparum isolates collected in 2021 from northern Uganda, where resistance-associated PfK13 C469Y and A675V mutations have emerged, and eastern Uganda, where these mutations are uncommon. With the ex vivo ring survival assay, isolates with the 469Y mutation (median survival 7.3% for mutant, 2.5% mixed, and 1.4% wild type) and/or mutations in Pfcoronin or falcipain-2a, had significantly greater survival; all isolates with survival >5% had mutations in at least one of these proteins. With ex vivo growth inhibition assays, susceptibility to lumefantrine (median IC50 14.6 vs. 6.9 nM, p < 0.0001) and dihydroartemisinin (2.3 vs. 1.5 nM, p = 0.003) was decreased in northern vs. eastern Uganda; 14/49 northern vs. 0/38 eastern isolates had lumefantrine IC50 > 20 nM (p = 0.0002). Targeted sequencing of 819 isolates from 2015–21 identified multiple polymorphisms associated with altered drug susceptibility, notably PfK13 469Y with decreased susceptibility to lumefantrine (p = 6 × 10−8) and PfCRT mutations with chloroquine resistance (p = 1 × 10−20). Our results raise concern regarding activity of artemether-lumefantrine, the first-line antimalarial in Uganda.
ΔFosB accumulation in hippocampal granule cells drives cFos pattern separation during spatial learning
Authors: Lamothe-Molina Paul J. , Franzelin Andreas , Beck Lennart , Li Dong , Auksutat Lea , Fieblinger Tim , Laprell Laura , Alhbeck Joachim , Gee Christine E. , Kneussel Matthias , Engel Andreas K. , Hilgetag Claus C. , Morellini Fabio , Oertner Thomas G. 
Published: October 2022

AbstractMice display signs of fear when neurons that express cFos during fear conditioning are artificially reactivated. This finding gave rise to the notion that cFos marks neurons that encode specific memories. Here we show that cFos expression patterns in the mouse dentate gyrus (DG) change dramatically from day to day in a water maze spatial learning paradigm, regardless of training level. Optogenetic inhibition of neurons that expressed cFos on the first training day affected performance days later, suggesting that these neurons continue to be important for spatial memory recall. The mechanism preventing repeated cFos expression in DG granule cells involves accumulation of ΔFosB, a long-lived splice variant of FosB. CA1 neurons, in contrast, repeatedly expressed cFos. Thus, cFos-expressing granule cells may encode new features being added to the internal representation during the last training session. This form of timestamping is thought to be required for the formation of episodic memories.
Discovery of conjoined charge density waves in the kagome superconductor CsV3Sb5
Authors: Li Haoxiang , Fabbris G. , Said A. H. , Sun J. P. , Jiang Yu-Xiao , Yin J.-X. , Pai Yun-Yi , Yoon Sangmoon , Lupini Andrew R. , Nelson C. S. , Yin Q. W. , Gong C. S. , Tu Z. J. , Lei H. C. , Cheng J.-G. , Hasan M. Z. , Wang Ziqiang , Yan Binghai , Thomale R. , Lee H. N. , Miao H. 
Published: October 2022

AbstractThe electronic instabilities in CsV3Sb5 are believed to originate from the V 3d-electrons on the kagome plane, however the role of Sb 5p-electrons for 3-dimensional orders is largely unexplored. Here, using resonant tender X-ray scattering and high-pressure X-ray scattering, we report a rare realization of conjoined charge density waves (CDWs) in CsV3Sb5, where a 2 × 2 × 1 CDW in the kagome sublattice and a Sb 5p-electron assisted 2 × 2 × 2 CDW coexist. At ambient pressure, we discover a resonant enhancement on Sb L1-edge (2s→5p) at the 2 × 2 × 2 CDW wavevectors. The resonance, however, is absent at the 2 × 2 × 1 CDW wavevectors. Applying hydrostatic pressure, CDW transition temperatures are separated, where the 2 × 2 × 2 CDW emerges 4 K above the 2 × 2 × 1 CDW at 1 GPa. These observations demonstrate that symmetry-breaking phases in CsV3Sb5 go beyond the minimal framework of kagome electronic bands near van Hove filling.
Biased signaling due to oligomerization of the G protein-coupled platelet-activating factor receptor
Authors: Liu Junke , Tang Hengmin , Xu Chanjuan , Zhou Shengnan , Zhu Xunying , Li Yuanyuan , Prézeau Laurent , Xu Tao , Pin Jean-Philippe , Rondard Philippe , Ji Wei , Liu Jianfeng 
Published: October 2022

AbstractG protein-coupled receptors (GPCRs) are important drug targets that mediate various signaling pathways by activating G proteins and engaging β-arrestin proteins. Despite its importance for the development of therapeutics with fewer side effects, the underlying mechanism that controls the balance between these signaling modes of GPCRs remains largely unclear. Here, we show that assembly into dimers and oligomers can largely influence the signaling mode of the platelet-activating factor receptor (PAFR). Single-particle analysis results show that PAFR can form oligomers at low densities through two possible dimer interfaces. Stabilization of PAFR oligomers through cross-linking increases G protein activity, and decreases β-arrestin recruitment and agonist-induced internalization significantly. Reciprocally, β-arrestin prevents PAFR oligomerization. Our results highlight a mechanism involved in the control of receptor signaling, and thereby provide important insights into the relationship between GPCR oligomerization and downstream signaling.
Aqueous hybrid electrochemical capacitors with ultra-high energy density approaching for thousand-volts alternating current line filtering
Authors: Li Zhou , Wang Xiaopeng , Zhao Lingyu , Chi Fengyao , Gao Chang , Wang Ying , Yan Mengdan , Zhou Qian , Zhao Miaomiao , Wang Xinyang , Wang Jiaqi , Yuan Man , Wu Mingmao , Wang Lixia , Zhao Yang , Qu Liangti 
Published: October 2022

AbstractFiltering capacitors with wide operating voltage range are essential for smoothing ripples in line-powered system, which are still unsatisfactory due to low energy density and limited working voltage scopes. Herein, we report an aqueous hybrid electrochemical capacitor with areal specific energy density of 1.29 mF V2 cm−2 at 120 Hz, greater than common aqueous ones. Interestingly, it can be easily integrated at scale to show excellent flexibility, controllable and stable filtering performance, in which an integrated device (e.g., seven units in series) exhibits fluctuation of 96 mV, 10 times smaller than an aluminum electrolytic capacitor with similar capacitance. A record-high 1,000 V can also be achieved after integrating 670 units, exceeding those reported so far, and about 1.5 times of commercial bulk aluminum electrolytic capacitors (~700 V). This work opens up a new insight for promising applications in multiple electricity transmission systems that requiring high smoothness under harsh voltage.
Evaporated nanometer chalcogenide films for scalable high-performance complementary electronics
Authors: Liu Ao , Zhu Huihui , Zou Taoyu , Reo Youjin , Ryu Gi-Seong , Noh Yong-Young 
Published: October 2022

AbstractThe exploration of stable and high-mobility semiconductors that can be grown over a large area using cost-effective methods continues to attract the interest of the electronics community. However, many mainstream candidates are challenged by scarce and expensive components, manufacturing costs, low stability, and limitations of large-area growth. Herein, we report wafer-scale ultrathin (metal) chalcogenide semiconductors for high-performance complementary electronics using standard room temperature thermal evaporation. The n-type bismuth sulfide delivers an in-situ transition from a conductor to a high-mobility semiconductor after mild post-annealing with self-assembly phase conversion, achieving thin-film transistors with mobilities of over 10 cm2 V−1 s−1, on/off current ratios exceeding 108, and high stability. Complementary inverters are constructed in combination with p-channel tellurium device with hole mobilities of over 50 cm2 V−1 s−1, delivering remarkable voltage transfer characteristics with a high gain of 200. This work has laid the foundation for depositing scalable electronics in a simple and cost-effective manner, which is compatible with monolithic integration with commercial products such as organic light-emitting diodes.
CD4 T cell epitope abundance in ferritin core potentiates responses to hemagglutinin nanoparticle vaccines
Authors: Nelson Sean A. , Richards Katherine A. , Glover Maryah A. , Chaves Francisco A. , Crank Michelle C. , Graham Barney S. , Kanekiyo Masaru , Sant Andrea J. 
Published: October 2022

AbstractNanoparticle vaccines based on H. pylori ferritin are increasingly used as a vaccine platform for many pathogens, including RSV, influenza, and SARS-CoV-2. They have been found to elicit enhanced, long-lived B cell responses. The basis for improved efficacy of ferritin nanoparticle vaccines remains unresolved, including whether recruitment of CD4 T cells specific for the ferritin component of these vaccines contributes to cognate help in the B cell response. Using influenza HA-ferritin nanoparticles as a prototype, we have performed an unbiased assessment of the CD4 T cell epitope composition of the ferritin particles relative to that contributed by influenza HA using mouse models that express distinct constellations of MHC class II molecules. The role that these CD4 T cells play in the B cell responses was assessed by quantifying follicular helper cells (TFH), germinal center (GC) B cells, and antibody secreting cells. When mice were immunized with equimolar quantities of soluble HA-trimers and HA-Fe nanoparticles, HA-nanoparticle immunized mice had an increased overall abundance of TFH that were found to be largely ferritin-specific. HA-nanoparticle immunized mice had an increased abundance of HA-specific isotype-switched GC B cells and HA-specific antibody secreting cells (ASCs) relative to mice immunized with soluble HA-trimers. Further, there was a strong, positive correlation between CD4 TFH abundance and GC B cell abundance. Thus, availability of helper CD4 T cell epitopes may be a key additional mechanism that underlies the enhanced immunogenicity of ferritin-based HA-Fe-nanoparticle vaccines.
Bending forces and nucleotide state jointly regulate F-actin structure
Authors: Reynolds Matthew J. , Hachicho Carla , Carl Ayala G. , Gong Rui , Alushin Gregory M. 
Published: October 2022

AbstractATP-hydrolysis-coupled actin polymerization is a fundamental mechanism of cellular force generation1–3. In turn, force4,5 and actin filament (F-actin) nucleotide state6 regulate actin dynamics by tuning F-actin’s engagement of actin-binding proteins through mechanisms that are unclear. Here we show that the nucleotide state of actin modulates F-actin structural transitions evoked by bending forces. Cryo-electron microscopy structures of ADP–F-actin and ADP-Pi–F-actin with sufficient resolution to visualize bound solvent reveal intersubunit interfaces bridged by water molecules that could mediate filament lattice flexibility. Despite extensive ordered solvent differences in the nucleotide cleft, these structures feature nearly identical lattices and essentially indistinguishable protein backbone conformations that are unlikely to be discriminable by actin-binding proteins. We next introduce a machine-learning-enabled pipeline for reconstructing bent filaments, enabling us to visualize both continuous structural variability and side-chain-level detail. Bent F-actin structures reveal rearrangements at intersubunit interfaces characterized by substantial alterations of helical twist and deformations in individual protomers, transitions that are distinct in ADP–F-actin and ADP-Pi–F-actin. This suggests that phosphate rigidifies actin subunits to alter the bending structural landscape of F-actin. As bending forces evoke nucleotide-state dependent conformational transitions of sufficient magnitude to be detected by actin-binding proteins, we propose that actin nucleotide state can serve as a co-regulator of F-actin mechanical regulation.