bluuebunny/crossref_metadata_2025_split huggingface dataset:
Body mass and triglycerides predict departure of free‐living nomadic pine siskins
Authors: DeSimone Joely G. , Domschot Beverly S. , Fylling Megan A. , Blake William M. , Breuner Creagh W. 
Published: November 2022

Abstract

Facultative migrations are observed across vertebrate taxa, include irruptive and nomadic movements, and occur in response to ephemeral and unpredictably variable resources. While the physiology underlying seasonal, obligate migrations is thoroughly studied, much less is known about the physiological mechanisms of facultative movements. We test two hypotheses in a free‐living, nomadic bird, the pine siskin (Spinus pinus).

The Prepare Hypothesis predicts that, like obligate migrants, siskins increase fuel stores to prepare for migratory movements and elevations of baseline corticosterone (CORT) support departure. The Escape Hypothesis predicts that siskins do not prepare for departure, body condition declines as food availability declines, and stress‐related levels of CORT induce escape from resource‐poor areas.

Under the controlled lab conditions of previous studies, food restriction induces declines in body condition and increases in CORT and locomotor activity, supporting the Escape Hypothesis. This study evaluates the ecological relevance of these captive findings by testing the Prepare and Escape Hypotheses in the field for the first time.

During two fall field seasons, we radio‐tagged siskins and tracked their local movements using handheld and automated telemetry. We assess how body condition and CORT relate to feeding behaviour [estimated via plasma triglycerides (TRIG)], space use and departure.

We do not find support for either the Prepare or Escape Hypothesis, but rather observe an intermediate pattern. Birds with higher TRIG, and therefore greater food intake, are more likely to depart. Birds in poor condition stay longer near the field site; however, above a threshold body mass, body condition does not predict departure.

These findings suggest moderate energy stores are necessary for departure, but movement decisions depend on other factors among birds with sufficient fuel. Siskin movements are physiologically distinct from both obligate and fugitive movements, and we discuss how food availability and body condition interact to drive different types of movement.

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Ablation of TRPC3 compromises bicarbonate and phosphate transporter activity in mice proximal tubular cells
Authors: Shin Samuel , Awuah Boadi Eugenia , Bandyopadhyay Bidhan C. 
Published: November 2022

AbstractProximal tubular (PT) cells reabsorb most calcium (Ca2+), phosphate (PO43−), bicarbonate (HCO3−), and oxalate (C2O42−) ions. We have shown that mice lacking Transient Receptor Potential Canonical 3 (TRPC3−/−) channel are moderately hypercalciuric with presentation of luminal calcium phosphate (CaP) crystals at the loop of Henle (LOH). However, other predisposing factors for such crystal deposition are unknown. Thus, we examined the distinctions in functional status of HCO3−, PO43−, and C2O42− transporters in PT cells of wild type (WT) and TRPC3−/− mice by whole‐cell patch clamp techniques to assess their contribution in the development of LOH CaP crystals. Here we show the development of concentration dependent HCO3−‐induced currents in all PT cells, which was confirmed by using specific HCO3− channel inhibitor, S0859. Interestingly, such activities were diminished in PT cells from TRPC3−/− mice, suggesting reduced HCO3− transport in absence of TRPC3. While PO43−‐induced currents were also concentration dependent in all PT cells (confirmed by PO43− channel inhibitor, PF‐06869206), those activities were reduced in absence of TRPC3, suggesting lower PO43− reabsorption that can leave excess luminal PO43−. Next, we applied thiosulfate (O3S22−) as a competitive inhibitor of the SLC26a6 transporter upon C2O42− current activation and observed a reduced C2O42−‐induced conductance which was greater in TRPC3−/− PT cells. Together, these results suggest that the reduced activities of HCO3−, PO43−, and C2O42− transporters in moderately hypercalciuric (TRPC3−/−) PT cells can create a predisposing condition for CaP and CaP tubular crystallization, enabling CaP crystal formation in LOH of TRPC3−/− mice.
Rhizopine biosensors for plant‐dependent control of bacterial gene expression
Authors: Haskett Timothy L. , Geddes Barney A. , Paramasivan Ponraj , Green Patrick , Chitnavis Samir , Mendes Marta D. , Jorrín Beatriz , Knights Hayley E. , Bastholm Tahlia R. , Ramsay Joshua P. , Oldroyd Giles E. D. , Poole Philip S. 
Published: November 2022

AbstractEngineering signalling between plants and microbes could be exploited to establish host‐specificity between plant‐growth‐promoting bacteria and target crops in the environment. We previously engineered rhizopine‐signalling circuitry facilitating exclusive signalling between rhizopine‐producing (RhiP) plants and model bacterial strains. Here, we conduct an in‐depth analysis of rhizopine‐inducible expression in bacteria. We characterize two rhizopine‐inducible promoters and explore the bacterial host‐range of rhizopine biosensor plasmids. By tuning the expression of rhizopine uptake genes, we also construct a new biosensor plasmid pSIR05 that has minimal impact on host cell growth in vitro and exhibits markedly improved stability of expression in situ on RhiP barley roots compared to the previously described biosensor plasmid pSIR02. We demonstrate that a sub‐population of Azorhizobium caulinodans cells carrying pSIR05 can sense rhizopine and activate gene expression when colonizing RhiP barley roots. However, these bacteria were mildly defective for colonization of RhiP barley roots compared to the wild‐type parent strain. This work provides advancement towards establishing more robust plant‐dependent control of bacterial gene expression and highlights the key challenges remaining to achieve this goal.
Fuel Poverty and the 2022 Energy Crisis
Authors: Simshauser Paul 
Published: November 2022

AbstractThe Ukraine war has increased coal and gas prices during 2022. Consequently, spot prices in Australia's National Electricity Market rose from $75 to $225/MWh, year‐on‐year. Households are shielded from spot prices, but as energy retailer hedge contracts mature, they are replaced by higher cost contracts, and end‐use retail tariffs will then rise. In this article, fuel poverty levels in Queensland are analysed. Model results forecast that fuel poverty rises from 6.8 per cent to 10.5 per cent of households. However, changes to energy concessions policy in 2016‒2017 materially enhanced horizontal and vertical efficiency, with successful targeting rising from 51 per cent to 69 per cent of vulnerable households.
The Diagnostic Value and Anchoring Effect of References in Acquisition Premium Decisions: The Influence of Overconfident and Powerful CEOs
Authors: Malhotra Shavin , Zhu Phil , Reus Taco H. 
Published: November 2022

AbstractCEOs often must make strategic decisions under extreme uncertainty. One way they deal with this uncertainty is by relying on references – that is, recent comparable decisions. However, there is a conundrum that underlies these references: they can have diagnostic value but also induce anchoring. We shed light on this conundrum by unpacking diagnostic value and anchoring effects of two commonly used references in acquisition premium decisions: an external reference (the premium paid for the preceding acquisition in the target industry) and an internal reference (the premium paid by a focal firm for its preceding acquisition). We theorize that while external references have diagnostic value and anchoring effects, internal references only have anchoring effects. Moreover, we argue that powerful and overconfident CEOs rely more on internal references. Our results, based on a hedonic regression analysis of 3072 completed acquisitions, support these hypotheses.
The Lettres Portugaises: Scripting and selling female desire
Authors: O'Leary Jessica 
Published: November 2022

AbstractThis article builds on previous literary scholarship to analyse the social and publication history of the enormously successful Lettres portugaises (1669), five letters published in the voice of an anonymous Portuguese nun to a French officer. Although the letters were based on an ancient model, this article suggests that their references to contemporary gendered constructions of biology and love, especially for enclosed women, were successfully used by publishers to commercialise a historically recurring gender binary of heterosexual love: men were rejected and women were abandoned. The popularity of the text was such that it entrenched notions of women's helplessness in matters of the heart for almost three centuries. This article argues that the Lettres portugaises’ success was as much the result of the text's literary qualities as it was of the canny paratextual strategies deployed by seventeenth‐ and eighteenth‐century publishers to sell the book, its sequels and its imitations.
Dimensions of effective government pandemic‐crisis communication in the context of COVID‐19: A public‐centric perspective
Authors: Kim Sora 
Published: November 2022

AbstractDrawing on two representative online surveys in Hong Kong (HK) and the United States (US) during the COVID‐19 pandemic, this study investigates, from a public‐centric perspective, public expectations of effective government pandemic‐crisis communication. The study looks specifically at what the publics want to be communicated in times of a global pandemic and how. In each region, the findings identify four significant dimensions. Three are culturally universal dimensions—basic responsibility, locus of pandemic‐crisis responsibility, and disfavour of promotional tone. The fourth is culture‐specific—personal relevance for HK and frequency for the US. Among the significant dimensions, the most highly expected is what people consider to be the government's basic responsibility in pandemic communication, that is, a basic responsibility dimension. This includes providing instructing and adjusting information and securing accuracy, timeliness, and transparency in pandemic communication. In both regions, respondents preferred by far traditional media and nongovernmental sources to social media and governmental sources.
Impact of alcohol policy changes on substance‐affected patients attending an emergency department in the Northern Territory with police
Authors: Brownlea Sandra , Miller Justine , Taylor Nicholas , Miller Peter , Coomber Kerri , Baldwin Ryan , Palmer Didier 
Published: November 2022

AbstractObjectiveAssess the impact of Northern Territory alcohol policy changes to ED utilisation at Royal Darwin‐Palmerston Regional Hospitals.MethodsInterrupted time series analysis explored trends in monthly ED attendance numbers and the proportion self‐discharging prior to policy changes (September 2016 to August 2017) and after three sequential interventions; the Banned Drinker Register, introduced September 2017, system changes to the sobering shelter, January 2018, and the minimum unit floor price (MUFP), October 2018. A targeted cohort of attendances transported by police as an alternative to the sobering shelter or police watch‐house when there is a medical concern was selected as they are likely impacted by all policy changes.ResultsPolice transported 1176 patients on 2070 occasions from September 2016 to March 2019. There was a downward trend in monthly attendances across the study period, with no significant change attributable to the Banned Drinker Register, a significant step decrease with the sobering shelter changes (P = 0.002), and a significant gradual decrease following the MUFP (P = 0.025). This represented an immediate decrease of 3.82 attendances per month/10 000 residents following the sobering shelter changes and a gradual decrease of 0.92 attendances/10 000 residents after the MUFP. Rates of self‐discharge were high, 45% in the pre‐intervention phase, decreasing to 28% following the MUFP but this trend did not reach significance with any intervention.ConclusionThe sequential introduction of broad sweeping alcohol policy changes introduced by the Northern Territory government was associated with significant reductions in ED utilisation. The proximity of the introduction of interventions creates difficulties identifying individual policy influence.
The frequency of wing damage in a migrating butterfly
Authors: Korkmaz Rabiya , Rajabi Hamed , Eshghi Shahab , Gorb Stanislav N. , Büscher Thies H. 
Published: November 2022

AbstractThe ability to fly is crucial for migratory insects. Consequently, the accumulation of damage on the wings over time can affect survival, especially for species that travel long distances. We examined the frequency of irreversible wing damage in the migratory butterfly Vanessa cardui to explore the effect of wing structure on wing damage frequency, as well as the mechanisms that might mitigate wing damage. An exceptionally high migration rate driven by high precipitation levels in their larval habitats in the winter of 2018–2019 provided us with an excellent opportunity to collect data on the frequency of naturally occurring wing damage associated with long‐distance flights. Digital images of 135 individuals of V. cardui were collected and analyzed in Germany. The results show that the hindwings experienced a greater frequency of damage than the forewings. Moreover, forewings experienced more severe damage on the lateral margin, whereas hindwings experienced more damage on the trailing margin. The frequency of wing margin damage was higher in the painted lady butterfly than in the migrating monarch butterfly and in the butterfly Pontia occidentalis following artificially induced wing collisions. The results of this study could be used in future comparative studies of patterns of wing damage in butterflies and other insects. Additional studies are needed to clarify whether the strategies for coping with wing damage differ between migratory and nonmigratory species.
Immunosuppressive, antimicrobial and insecticidal activities of inhibitor cystine knot peptides produced by teratocytes of the endoparasitoid wasp Cotesia flavipes (Hymenoptera: Braconidae)
Authors: Pinto Ciro P. G. , Walker Andrew A. , King Glenn F. , Rossi Guilherme D. 
Published: November 2022

AbstractTeratocytes are specialized cells released by parasitoid wasps into their hosts. They are known for producing regulatory molecules that aid the development of immature parasitoids. We have recently reported the primary structures of cystine‐rich peptides, including some containing inhibitor cystine knot (ICK) motifs, produced by teratocytes of the parasitoid Cotesia flavipes (Hymenoptera: Braconidae). ICKs are known for their stability and diverse biological functions. In this study, we produced four putative ICK peptides from the teratocytes of C. flavipes using solid‐phase peptide synthesis or recombinant expression in E. coli, and investigated their functions on host immune modulation as well their potential to impair the development of two lepidopterans after ingestion of the peptides. In addition, the peptides were assayed against pathogens and human cells. The peptides did not influence total hemocyte count but suppressed cellular immunity, detectable as a reduction of hemocyte encapsulation (CftICK‐I, CftICK‐II, CftICK‐III) and spread indexes (CftICK‐IV) in the host. None of the peptides influenced the activities of prophenoloxidase and phenoloxidase in the hemolymph of larval Diatraea saccharalis (Lepidoptera: Crambidae). CftICK‐I and CftICK‐II with previously unknown function showed antifungal activity against Candida albicans but were non‐toxic to human cells. CftICK‐I, CftICK‐II, and CftICK‐III increased larval mortality and reduced leaf consumption of D. saccharalis, a permissive host for C. flavipes. The CftICK‐III also increased larval mortality and reduced leaf consumption of Spodoptera frugiperda (Lepidoptera: Noctuidae), a non‐permissive host for C. flavipes. This study highlights biological functions and biotechnological potential of ICK peptides from the teratocytes of C. flavipes.
Operationalizing equity for integrated water resources management
Authors: Seigerman Cydney K. , McKay S. Kyle , Basilio Raul , Biesel Shelly A. , Hallemeier Jon , Mansur Andressa V. , Piercy Candice , Rowan Sebastian , Ubiali Bruno , Yeates Elissa , Nelson Donald R. 
Published: November 2022

AbstractAdvancing social equity has been implicitly and explicitly central to water resources policy for decades. Yet, equity remains largely outside of standard water resources planning and management practices. Inclusion of equity within water resources infrastructure is inhibited by barriers including an incomplete conceptual understanding of equity, a perceived lack of quantitative and qualitative equity metrics, unclear connections between equity and standard project planning frameworks, and the absence of concrete examples. To facilitate greater practical inclusion of social equity in water resources practices, we describe equity relative to dimensions of distribution, procedure, and recognition and identify metrics associated with each. We then map these dimensions of equity onto different stages of a water resources project life cycle. We discuss how inequities are often perpetuated by current approaches and highlight case studies that promote one or more of the facets of equity. Rather than providing a prescriptive solution to “achieve” equity within water resources practices, we emphasize the need for contextualized approaches that include pragmatic steps toward more equitable practices and outcomes.
Minimal important difference in opioid consumption based on adverse event reduction—A study protocol
Authors: Karlsen Anders Peder Højer , Pedersen Casper , Laigaard Jens , Thybo Kasper Højgaard , Gasbjerg Kasper Smidt , Geisler Anja , Lunn Troels Haxholdt , Hägi‐Pedersen Daniel , Jakobsen Janus Christian , Mathiesen Ole 
Published: November 2022

AbstractBackgroundThe patient‐relevant minimal important difference for opioid consumption remains undetermined, despite its frequent use as primary outcome in trials on postoperative pain management. A minimal important difference is necessary to evaluate whether significant trial results are clinically relevant. Further, it can be used as effect size to ensure that trials are powered to find clinically relevant effects. By exploring the dose–response relationship between postoperative opioid consumption and opioid‐related adverse effects, we aim to approximate the minimal important difference in opioid consumption anchored to opioid‐related adverse effects.MethodsThis is a post‐hoc analysis of aggregated data from two clinical trials (PANSAID NCT02571361 and DEX2TKA NCT03506789) and one observational cohort study (Pain Map NCT02340052) on pain management after total hip and knee arthroplasty. The primary outcome is the Hodges–Lehmann median difference in opioid consumption between patients with no opioid‐related adverse effects and patients experiencing the mildest degree of one or more opioid‐related adverse effects (i.e., mild nausea, sedation and/or dizziness or vomiting). Secondary outcomes include the Hodges–Lehmann median difference in opioid consumption that corresponds to one point on a cumulated opioid‐related adverse event 0–10 scale. Further, we will explore the proportion of patients that experience opioid‐related adverse effects for consecutive opioid dose intervals of 2 mg iv morphine equivalents. Quantile regression will be used to assess any significant interactions with patient baseline characteristics.ConclusionsThis study will hopefully bring us one step closer to determining relevant opioid reductions and thereby improve our understanding of intervention effects and planning of future trials.
Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
Authors: Whitehead Meredith , Yusoff Syabira , Ahmad Sadia , Schmidt Lukas , Mayr Manuel , Madine Jillian , Middleton David , Shanahan Catherine M. 
Published: November 2022

AbstractVascular amyloidosis, caused when peptide monomers aggregate into insoluble amyloid, is a prevalent age‐associated pathology. Aortic medial amyloid (AMA) is the most common human amyloid and is composed of medin, a 50‐amino acid peptide. Emerging evidence has implicated extracellular vesicles (EVs) as mediators of pathological amyloid accumulation in the extracellular matrix (ECM). To determine the mechanisms of AMA formation with age, we explored the impact of vascular smooth muscle cell (VSMC) senescence, EV secretion, and ECM remodeling on medin accumulation. Medin was detected in EVs secreted from primary VSMCs. Small, round medin aggregates colocalized with EV markers in decellularized ECM in vitro and medin was shown on the surface of EVs deposited in the ECM. Decreasing EV secretion with an inhibitor attenuated aggregation and deposition of medin in the ECM. Medin accumulation in the aortic wall of human subjects was strongly correlated with age and VSMC senescence increased EV secretion, increased EV medin loading and triggered deposition of fibril‐like medin. Proteomic analysis showed VSMC senescence induced changes in EV cargo and ECM composition, which led to enhanced EV‐ECM binding and accelerated medin aggregation. Abundance of the proteoglycan, HSPG2, was increased in the senescent ECM and colocalized with EVs and medin. Isolated EVs selectively bound to HSPG2 in the ECM and its knock‐down decreased formation of fibril‐like medin structures. These data identify VSMC‐derived EVs and HSPG2 in the ECM as key mediators of medin accumulation, contributing to age‐associated AMA development.
Does state repeal of alcohol exclusion laws increase problem drinking?
Authors: Azagba Sunday , Shan Lingpeng , Ebling Todd , Wolfson Mark , Hall Mark , Chaloupka Frank 
Published: November 2022

AbstractBackgroundFor decades, alcohol exclusion laws (AELs) have allowed insurance companies to reject claims for physical injuries caused by alcohol consumption, including injuries from impaired driving. A central premise of AELs is that they function as a deterrent to risk‐taking behaviors, such as excessive drinking. If this assumption is correct, state repeal of these laws should result in increased drinking. This study examines whether the repeal of AELs by some states affects drinking behaviors.MethodsData were obtained from the 1993 to 2017 Behavioral Risk Factor Surveillance System nationwide survey. Exploiting the natural experiment presented by state repeal of AELs, we assessed the impact on current drinking and binge drinking. We used a rigorous quasi‐experimental difference‐in‐differences analysis and conducted a battery of sensitivity analyses to assure robust findings.ResultsOverall, the study found no discernable impact of state repeal of AELs on alcohol consumption. While the repeal of AELs significantly decreased the odds of reporting drinking in the past 30 days compared to those living in states with AELs or that never had AELs, the effects were small (aOR = 0.98, 95% CI = 0.96, 0.99). Likewise, there were higher odds of binge drinking among individuals living in states that repealed AELs compared to those living in states without AELs, yet with small effects (aOR = 1.03, 95% CI = 1.01, 1.05). After additionally adjusting for state‐varying characteristics and state‐specific time trends, no significant effects were identified regarding current and binge drinking. Findings from the sensitivity analyses were largely consistent with the main analysis.ConclusionThis study found no evidence supporting the idea that repealing AELs increased alcohol consumption or binge drinking. Future studies should consider other state‐specific dimensions within the Uniform Accident and Sickness Policy Provision Law.