bluuebunny/crossref_metadata_2025_split huggingface dataset:
An insight in proteome profiling of Tuta absoluta larvae after entomopathogenic fungal infection
Authors: Bali Gurmeet Kour , Singh Sanjay Kumar , Chauhan Vinod Kumar , Joshi Neha , Bhat Firdous Ahmad , Malla Waseem Akram , Ramanujam Boman , Varshney Richa , Kour Manpreet , Pandit Radhakrishnan Sitaram 
Published: August 2022

AbstractTuta absoluta (L.) (Lepidoptera: Gelechiidae), a major pest of solanaceous plant species, causes serious losses in the agriculture sector around the globe. For better pest management, entomopathogenic fungi such as Beauveria bassiana and Purpureocillium lilacinum, play an efficient role in suppressing the pest population. The present study was carried out to analyse the effects post fungal infections through proteome profiling using an Orbitrap Fusion Tribrid mass spectrometer. A total of 2,201 proteins were identified from the fourth instar larvae of T. absoluta, of which 442 and 423 proteins were significantly dysregulated upon infection with P. lilacinum and B. bassiana respectively. The potential proteins related to immune systems as well as detoxification processes showed significant alterations after post fungal infection. Studies on T. absoluta proteomics and genomics as well as the consequences of entomopathogenic fungal infection on the immune response of this insect could provide an initial framework for exploring more fungus-host interactions for the development of better strategies for integrated pest management.
Aurignacian dynamics in Southeastern Europe based on spatial analysis, sediment geochemistry, raw materials, lithic analysis, and use-wear from Românești-Dumbrăvița
Authors: Chu Wei , McLin Scott , Wöstehoff Luisa , Ciornei Alexandru , Gennai Jacopo , Marreiros João , Doboș Adrian 
Published: August 2022

AbstractThe Aurignacian is one of the first cultural-technological traditions commonly associated with the expansion ofHomo sapiensin Europe. EarlyHomo sapiensdemographics across the continent are therefore typically inferred using the distribution of Aurignacian assemblages. Western Romania has been used as a tie-point to connect the well-researched lithic assemblages from the eastern Mediterranean and Western Europe through its earlyHomo sapiensfossils. However, Romania’s archeological record remains underexplored thereby hindering our ability to directly connect better understood regions through time and space. Here we report on excavations from the open-air Middle/Upper Paleolithic site of Româneș﻿ti-DumbrăvițaI in southwestern Romania. Three stratified Paleolithic assemblages were extensively excavated within a 1-m-thick eolian-deposited sequence. Spatial, geochemical, raw material, techno-typological, and use-wear analysis of the site reveal patterns of artifact configuration, resource exploitation, fire history, knapping objectives, and functionality. Taken together, Românești-DumbrăvițaI is the first well-contextualized archeological site in close spatiotemporal proximity to many early, well-preserved human fossils and in East-Central Europe.
Spatiotemporal variation evaluation of water quality in middle and lower Han River, China
Authors: Deng Lele , Chen Kebing , Liu Zhangjun , Wu Boyang , Chen Zekun , He Shaokun 
Published: August 2022

AbstractAs the water source for the middle route of the South-to-North Water Transfer Project, the Han River in China plays a role of the world’s largest inter-basin water transfer project. However, this human-interfered area has suffered from over-standard pollution emission and water blooms in recent years, which necessitates urgent awareness at both national and provincial scales. To perform a comprehensive analysis of the water quality condition of this study area, we apply both the water quality index (WQI) and minimal WQI (WQImin) methods to investigate the spatiotemporal variation characteristics of water quality. The results show that 8 parameters consisting of permanganate index (PI), chemical oxygen demand (COD), total phosphorus (TP), fluoride (F-), arsenic (As), plumbum (Pb), copper (Cu), and zinc (Zn) have significant discrepancy in spatial scales, and the study basin also has a seasonal variation pattern with the lowest WQI values in summer and autumn. Moreover, compared to the traditional WQI, the WQImin model, with the assistance of stepwise linear regression analysis, could exhibit more accurate explanation with the coefficient of determination (R2) and percentage error (PE) values being 0.895 and 5.515%, respectively. The proposed framework is of great importance to improve the spatiotemporal recognition of water quality patterns and further helps develop efficient water management strategies at a reduced cost.
Increased shark bite survivability revealed by two centuries of Australian records
Authors: Tucker James P. , Santos Isaac R. , Kelaher Brendan P. , Green Marcel , Clark Graeme F. , Butcher Paul A. 
Published: August 2022

AbstractThe perceived and real threat of shark bites have significant direct health and indirect economic impacts. Here we assess the changing odds of surviving an unprovoked shark bite using 200 years of Australian records. Bite survivability rates for bull (Carcharhinus leucas), tiger (Galeocerdo cuvier) and white (Carcharodon carcharias) sharks were assessed relative to environmental and anthropogenic factors. Survivability of unprovoked bull, tiger and white shark bites were 62, 75 and 53% respectively. Bull shark survivability increased over time between 1807 and 2018. Survivability decreased for both tiger and white sharks when the person was doing an in water activity, such as swimming or diving. Not unsurprisingly, a watercraft for protection/floatation increased survivability to 92% from 30%, and 88% from 45%, for tiger and white sharks respectively. We speculate that survival may be related to time between injury and treatment, indicating the importance of rapid and appropriate medical care. Understanding the predictors of unprovoked bites, as well as survivability (year and water activity), may be useful for developing strategies that reduce the number of serious or fatal human-shark interactions without impacting sharks and other marine wildlife.
Analysis of integrated UWB MIMO and CR antenna system using transmission line model with functional verification
Authors: Prabhu P. , Subramani Malarvizhi , Kwak Kyung-sup 
Published: August 2022

AbstractThis paper discusses the equivalent circuit model and functional verification of an integrated antenna system as its main focus. The integrated antenna system consists of two independent antenna systems, namely the Cognitive Radio antenna and the Ultra Wide Band Multiple Iinput Multiple Output antenna. This article is split into two parts: The first part discusses the equivalent circuit of an integrated antenna system by optimizing the RLC values. The developed lumped equivalent circuit model produces the NB resonant frequencies, which is the same as the S-parameter obtained through EM simulation. The second part of this paper aims to discuss the experimental verification of an integrated CR antenna system with Bayesian learning-based spectrum sensing algorithms using Universal Software Radio Peripheral devices. Real-time sensing and communication functionalities are visualized in the LABVIEW monitor. The integrated antenna system is fabricated and measured after the simulation.
Neurofilament light chain in central nervous system infections: a prospective study of diagnostic accuracy
Authors: van Zeggeren Ingeborg E. , ter Horst Liora , Heijst Hans , Teunissen Charlotte E. , van de Beek Diederik , Brouwer Matthijs C. 
Published: August 2022

AbstractDiagnosing central nervous system (CNS) infections quickly is often difficult. Neurofilament light chain (NfL) is a component of the axonal cytoskeleton and identified as marker of neuronal damage in several CNS diseases. We evaluated the diagnostic accuracy of NfL for diagnosing CNS infections. We included patients from a prospective cohort of consecutive patients in whom a lumbar puncture was performed for suspected CNS infection in an academic hospital in The Netherlands. The index test was NfL in cerebrospinal fluid (CSF) and reference standard the final clinical diagnosis. Diagnostic accuracy was determined using the area-under-the-curve (AUC) with 95% confidence intervals (CI). The association of CSF NfL with clinical characteristics, diagnosis and outcome was evaluated. Between 2012 and 2015, 273 episodes in adults of which sufficient CSF was available were included. CNS infection was diagnosed in 26%(n = 70), CNS inflammatory disease in 7%(n = 20), systemic infection in 32%(n = 87), and other neurological disorders in 33%(n = 90). Median CSF NfL level was 593 pg/ml (IQR 249–1569) and did not discriminate between diagnostic categories or CNS infection subcategories. AUC for diagnosing any CNS infection compared to patients without CNS infections was 0.50 (95% CI 0.42–0.59). Patients presenting with an altered mental status had higher NfL levels compared to other patients. We concluded that NfL cannot discriminate between causes in patients suspected of CNS infections. High concentrations of NfL are associated with severe neurological disease and the prognostic value of NfL in patients with CNS infections should be investigated in future research.
Sample-centred shimming enables independent parallel NMR detection
Authors: Cheng Yen-Tse , Jouda Mazin , Korvink Jan 
Published: August 2022

AbstractTwo major technical challenges facing parallel nuclear magnetic resonance (NMR) spectroscopy, at the onset, include the need to achieve exceptional $$B_0$$

B
0

homogeneity, and good inter-detector radiofrequency signal decoupling, and have remained as technical obstacles that limit high throughput compound screening via NMR. In this contribution, we consider a compact detector system, consisting of two NMR ‘unit cell’ resonators that implement parallel $$B_0$$

B
0

shimming with parallel radiofrequency detection, as a prototype NMR environment, pointing the way towards achieving accelerated NMR analysis. The utility of our approach is established by achieving local field correction within the bore of a 1.05T permanent magnet MRI. Our forerunner platform suppresses signal cross-coupling in the range of $$-21$$

-
21

dB to $$-30$$

-
30

dB, under a geometrically decoupled scheme, leading to a halving of the necessary inter-coil separation. In this permanent magnet environment, two decoupled parallel NMR detector sites simultaneously achieve narrow spectral linewidth, overcoming the spatial inhomogeneity of the magnet from 400 to 28 Hz.
Limbal epithelial stem cell sheets from young donors have better regenerative potential
Authors: Yang Soonwon , Lee Hyun Jung , Shin Soojung , Park In Yang , Chung So-Hyang 
Published: August 2022

AbstractTo investigate the stemness of limbal epithelial stem cell sheets in relation to the donor’s age. Human limbal explants from cadaveric donors were set on human amniotic membrane scaffolds with the xeno-free medium. We evaluated limbal epithelial sheet size, expression of stem/progenitor cell markers, and colony formation efficiency from donors of different age groups (age ≤ 45, age 45–65, and age > 65). Expression of the proliferation marker Ki67, stem/progenitor cell markers p63α and ABCG2, cornea specific marker PANCK, and differentiation marker CK12 were evaluated. To determine the effect of donor age on the storage period of limbal explant sheets, the limbal explant outgrowth sheets were stored in 4 °C for 2 days and analyzed for JC-1, p63α, and PANCK with FACS on each day. From days 6 to 12, the outgrowth area of the limbal epithelial stem cell sheet was significantly larger in the age ≤ 45 groups (296 ± 54.7 mm2, day 9) compared to the other two age groups [age 45–65 group (278 ± 62.6 mm2), age > 65 group (257 ± 44.0 mm2), day 9] (p < 0.01). In terms of stemness, outgrowth cells from aged donors (age > 65) showed lower expression of stem/progenitor cell markers p63α and ABCG2 and decreased CFE compared to the other two groups. There were significantly more p63α+ cells in outgrowth cells in the age ≤ 45 group (18.2 ± 3.6%) compared to the age > 65 group (14.1 ± 4.6%; p < 0.01). Limbal explant outgrowth sheet on the age ≤ 45 group (32.7 ± 7.5%) had higher percentages of cells resisting staining by JC-1 compared with sheets under the age > 65 groups (25.7 ± 7.1%, p < 0.01) (JC-1low). Cells from the age ≤ 45 group showed a higher clonogenic capacity than those from the other two age groups (45 < Age ≤ 65 CFE ratio = 0.7 ± 0.16, p < 0.01; 65 < Age CFE ratio = 0.3 ± 0.06, p < 0.01, vs. Age ≤ 45). In the age > 65 group, positive cells of p63α on D0, 1, and 2 were significantly lower compared to those in the age ≤ 45 group on the storage period (p < 0.01, respectively). Our results imply that donors younger than 65 years of age are a better source of limbal epithelial stem cell sheet generation with high regeneration potential.
Biological and chemical properties of five mineral oxides and of mineral trioxide aggregate repair high plasticity: an in vitro study
Authors: Abu Hasna Amjad , de Paula Ramos Lucas , Campos Tiago Moreira Bastos , de Castro Lopes Sergio Lucio Pereira , Rachi Maisour Ala , de Oliveira Luciane Dias , Carvalho Cláudio Antonio Talge 
Published: August 2022

AbstractCalcium silicate-based cements have diverse applications in endodontics. This study aimed to evaluate the antibiofilm action, biocompatibility, morphological structure, chemical composition and radiopacity of Five Mineral Oxides (5MO), Mineral Trioxide Aggregate Repair High Plasticity (MTA Repair HP), and Mineral Trioxide Aggregate (MTA) cements. MTT analysis was used to test the antibiofilm action of these cements against five anaerobic microorganisms, and test their biocompatibility with mouse macrophage (RAW 264.7) and osteoblasts (MG-63) cultures. Their morphological structure and chemical composition were evaluated by scanning electron microscopy (SEM) coupled to energy dispersion X-ray spectroscopy (EDX), and the phase analysis was performed by X-ray diffraction (XRD). Conventional radiography was used to assess the radiopacity of the cements. 5MO, MTA Repair HP and MTA were effective against Porphyromonas gingivalis, Parvimonas micra, Fusobacterium nucleatum and Prevotella intermedia, they were biocompatible with macrophages and osteoblasts after 5 min of contact, and they had adequate radiopacity to be used clinically. Bismuth oxide (Bi2O3) is used as a radiopacifier in MTA and 5MO, and calcium tungstate, in MTA Repair HP. Titanium dioxide (TiO2) (ANATASE) is responsible for the antimicrobial action and biocompatibility of 5MO.
Biophysical and pharmacokinetic characterization of a small-molecule inhibitor of RUNX1/ETO tetramerization with anti-leukemic effects
Authors: Gopalswamy Mohanraj , Kroeger Tobias , Bickel David , Frieg Benedikt , Akter Shahina , Schott-Verdugo Stephan , Viegas Aldino , Pauly Thomas , Mayer Manuela , Przibilla Julia , Reiners Jens , Nagel-Steger Luitgard , Smits Sander H. J. , Groth Georg , Etzkorn Manuel , Gohlke Holger 
Published: August 2022

AbstractAcute myeloid leukemia (AML) is a malignant disease of immature myeloid cells and the most prevalent acute leukemia among adults. The oncogenic homo-tetrameric fusion protein RUNX1/ETO results from the chromosomal translocation t(8;21) and is found in AML patients. The nervy homology region 2 (NHR2) domain of ETO mediates tetramerization; this oligomerization is essential for oncogenic activity. Previously, we identified the first-in-class small-molecule inhibitor of NHR2 tetramer formation, 7.44, which was shown to specifically interfere with NHR2, restore gene expression down-regulated by RUNX1/ETO, inhibit the proliferation of RUNX1/ETO-depending SKNO-1 cells, and reduce the RUNX1/ETO-related tumor growth in a mouse model. However, no biophysical and structural characterization of 7.44 binding to the NHR2 domain has been reported. Likewise, the compound has not been characterized as to physicochemical, pharmacokinetic, and toxicological properties. Here, we characterize the interaction between the NHR2 domain of RUNX1/ETO and 7.44 by biophysical assays and show that 7.44 interferes with NHR2 tetramer stability and leads to an increase in the dimer population of NHR2. The affinity of 7.44 with respect to binding to NHR2 is Klig = 3.75 ± 1.22 µM. By NMR spectroscopy combined with molecular dynamics simulations, we show that 7.44 binds with both heteroaromatic moieties to NHR2 and interacts with or leads to conformational changes in the N-termini of the NHR2 tetramer. Finally, we demonstrate that 7.44 has favorable physicochemical, pharmacokinetic, and toxicological properties. Together with biochemical, cellular, and in vivo assessments, the results reveal 7.44 as a lead for further optimization towards targeted therapy of t(8;21) AML.
Neuronal gating of tactile input and sleep in 10-month-old infants at typical and elevated likelihood for autism spectrum disorder
Authors: De Laet Anna , Piccardi Elena Serena , Begum-Ali Jannath , Charman Tony , Johnson Mark H. , Jones Emily J. H. , Bedford Rachael , Gliga Teodora ,   The STAARS Team, Agyapong Mary , Bazelmans Tessel , Dafner Leila , Ersoy Mutluhan , Goodwin Amy , Haartsen Rianne , Halkola Hanna , Hendry Alexandra , Holman Rebecca , Kalwarowsky Sarah , Kolesnik-Taylor Anna , Lloyd-Fox Sarah , Mason Luke , Narvekar Nisha , Pasco Greg , Pirazzoli Laura , Taylor Chloë 
Published: August 2022

AbstractSleep problems in Autism Spectrum Disorder (ASD) emerge early in development, yet the origin remains unclear. Here, we characterise developmental trajectories in sleep onset latency (SOL) and night awakenings in infants at elevated likelihood (EL) for ASD (who have an older sibling with ASD) and infants at typical likelihood (TL) for ASD. Further, we test whether the ability to gate tactile input, using an EEG tactile suppression index (TSI), associates with variation in SOL and night awakenings. Parent-reported night awakenings and SOL from 124 infants (97 at EL for ASD) at 5, 10 and 14 months were analyzed using generalized estimating equations. Compared to TL infants, infants at EL had significantly more awakenings and longer SOL at 10 and 14 months. The TSI predicted SOL concurrently at 10 months, independent of ASD likelihood status, but not longitudinally at 14 months. The TSI did not predict night awakenings concurrently or longitudinally. These results imply that infants at EL for ASD wake up more frequently during the night and take longer to fall asleep from 10 months of age. At 10 months, sensory gating predicts SOL, but not night awakenings, suggesting sensory gating differentially affects neural mechanisms of sleep initiation and maintenance.
Prediction of mortality risk of health checkup participants using machine learning-based models: the J-SHC study
Authors: Kawano Kazuharu , Otaki Yoichiro , Suzuki Natsuko , Fujimoto Shouichi , Iseki Kunitoshi , Moriyama Toshiki , Yamagata Kunihiro , Tsuruya Kazuhiko , Narita Ichiei , Kondo Masahide , Shibagaki Yugo , Kasahara Masato , Asahi Koichi , Watanabe Tsuyoshi , Konta Tsuneo 
Published: August 2022

AbstractEarly detection and treatment of diseases through health checkups are effective in improving life expectancy. In this study, we compared the predictive ability for 5-year mortality between two machine learning-based models (gradient boosting decision tree [XGBoost] and neural network) and a conventional logistic regression model in 116,749 health checkup participants. We built prediction models using a training dataset consisting of 85,361 participants in 2008 and evaluated the models using a test dataset consisting of 31,388 participants from 2009 to 2014. The predictive ability was evaluated by the values of the area under the receiver operating characteristic curve (AUC) in the test dataset. The AUC values were 0.811 for XGBoost, 0.774 for neural network, and 0.772 for logistic regression models, indicating that the predictive ability of XGBoost was the highest. The importance rating of each explanatory variable was evaluated using the SHapley Additive exPlanations (SHAP) values, which were similar among these models. This study showed that the machine learning-based model has a higher predictive ability than the conventional logistic regression model and may be useful for risk assessment and health guidance for health checkup participants.
Interaction effects of multimorbidity and frailty on adverse health outcomes in elderly hospitalised patients
Authors: Lujic Sanja , Randall Deborah A. , Simpson Judy M. , Falster Michael O. , Jorm Louisa R. 
Published: August 2022

AbstractWe quantified the interaction of multimorbidity and frailty and their impact on adverse health outcomes in the hospital setting. Using aretrospective cohort study of persons aged ≥ 75 years, admitted to hospital during 2010–2012 in New South Wales, Australia, and linked with mortality data, we constructed multimorbidity, frailty risk and outcomes: prolonged length of stay (LOS), 30-day mortality and 30-day unplanned readmissions. Relative risks (RR) of outcomes were obtained using Poisson models with random intercept for hospital. Among 257,535 elderly inpatients, 33.6% had multimorbidity and elevated frailty risk, 14.7% had multimorbidity only, 19.9% had elevated frailty risk only and 31.8% had neither. Additive interactions were present for all outcomes, with a further multiplicative interaction for mortality and LOS. Mortality risk was 4.2 (95% CI 4.1–4.4), prolonged LOS 3.3 (95% CI 3.3–3.4) and readmission 1.8 (95% CI 1.7–1.9) times higher in patients with both factors present compared with patients with neither. In conclusion, multimorbidity and frailty coexist in older hospitalized patients and interact to increase the risk of adverse outcomes beyond the sum of their individual effects. Their joint effect should be considered in health outcomes research and when administering hospital resources.
Understanding geographic and racial/ethnic disparities in mortality from four major cancers in the state of Georgia: a spatial epidemiologic analysis, 1999–2019
Authors: Moore Justin Xavier , Tingen Martha S. , Coughlin Steven S. , O’Meara Christine , Odhiambo Lorriane , Vernon Marlo , Jones Samantha , Petcu Robert , Johnson Ryan , Islam K. M. , Nettles Darryl , Albashir Ghadeer , Cortes Jorge 
Published: August 2022

AbstractWe examined geographic and racial variation in cancer mortality within the state of Georgia, and investigated the correlation between the observed spatial differences and county-level characteristics. We analyzed county-level cancer mortality data collected by the Centers for Disease Control and Prevention on breast, colorectal, lung, and prostate cancer mortality among adults (aged ≥ 18 years) in 159 Georgia counties from years 1999 through 2019. Geospatial methods were applied, and we identified hot spot counties based on cancer mortality rates overall and stratified by non-Hispanic white (NH-white) and NH-black race/ethnicity. Among all adults, 5.0% (8 of 159), 8.2% (13 of 159), 5.0% (8 of 159), and 6.9% (11 of 159) of Georgia counties were estimated hot spots for breast cancer, colorectal, lung, and prostate cancer mortality, respectively. Cancer mortality hot spots were heavily concentrated in three major areas: (1) eastern Piedmont to Coastal Plain regions, (2) southwestern rural Georgia area, or (3) northern-most rural Georgia. Overall, hot spot counties generally had higher proportion of NH-black adults, older adult population, greater poverty, and more rurality. In Georgia, targeted cancer prevention strategies and allocation of health resources are needed in counties with elevated cancer mortality rates, focusing on interventions suitable for NH-black race/ethnicity, low-income, and rural residents.