bluuebunny/crossref_metadata_2025_split huggingface dataset:
WIRID VERSES TO STRENGTHEN MEMORIZATION: STUDY OF LIVING QUR’AN READING SELECTED VERSES OF SURAH AL-BAQARAH AT PONDOK PESANTREN
Authors: Fanjah Ilfi Nur Faizatul , Ulwiyah Robiatul , Rahmawati Kharolina , Al Masithoh Silvinatin , Rahman Azibur 
Published: September 2022

Having vital memorization or memory is the dream of everyone, including those who want to memorize the Qur’an. However, the facts on the ground are that not all of these memories are directly proportional to everyone’s wishes. One way and effort to have vital memorization is wirid from the Qur’an, as practiced at the Darussalam Islamic Boarding School in Blokagung Banyuwangi, which uses the wirid surah Al-Baqarah. Based on the practices and habits of the Darussalam Islamic boarding school, this research was appointed with a focus on studying the living Qur’an. It is hoped that later an answer can be found from the formula: how does the wirid al-Qur’an (Al-Baqarah) come alive and affect the sharpness of a santri’s memorization? As a methodical step, this research uses a descriptive-qualitative method based on a phenomenon (phenomenological). The results of this study conclude that this memorizing wirid becomes a medium to get closer to Allah by using dhikr taken from pieces of the verses of the Qur'an Surah al-Baqarah has given confidence to its practitioners as a reinforcement for memorizing the Qur’an. 'an and have made the Qur’an.
Dynamic features of human mitochondrial DNA maintenance and transcription
Authors: Akbari Mansour , Nilsen Hilde Loge , Montaldo Nicola Pietro 
Published: September 2022

Mitochondria are the primary sites for cellular energy production and are required for many essential cellular processes. Mitochondrial DNA (mtDNA) is a 16.6 kb circular DNA molecule that encodes only 13 gene products of the approximately 90 different proteins of the respiratory chain complexes and an estimated 1,200 mitochondrial proteins. MtDNA is, however, crucial for organismal development, normal function, and survival. MtDNA maintenance requires mitochondrially targeted nuclear DNA repair enzymes, a mtDNA replisome that is unique to mitochondria, and systems that control mitochondrial morphology and quality control. Here, we provide an overview of the current literature on mtDNA repair and transcription machineries and discuss how dynamic functional interactions between the components of these systems regulate mtDNA maintenance and transcription. A profound understanding of the molecular mechanisms that control mtDNA maintenance and transcription is important as loss of mtDNA integrity is implicated in normal process of aging, inflammation, and the etiology and pathogenesis of a number of diseases.
Nanochannel array modified three-dimensional graphene electrode for sensitive electrochemical detection of 2,4,6-trichlorophenol and prochloraz
Authors: Zheng Weiran , Su Ruobing , Lin Xingyu , Liu Jiyang 
Published: September 2022

Convenient, and sensitive detection of pesticides and their metabolites in environmental or food samples is critical for assessing potential environmental and health risks. Here, a three-dimensional (3D) electrochemical sensing platform is proposed based on the integration of nanochannel array on pre-activated 3D graphene (p-3DG) electrodes with no need of additional adhesive layers, which enables sensitive detection of prochloraz and 2,4,6-trichlorophenol (TCP) in environmental and food samples. Through two-step electrochemical polarization, organic phase anodic oxidation, and aqueous phase cathodic reduction, p-3DG electrodes with high active area and excellent electrocatalytic performance were obtained. Vertically-ordered mesoporous silica-nanochannel film (VMSF) can be rapidly grown on the surface of p-3DG by an electrochemical-assisted self-assembly (EASA) method. Taking advantage of the high electrocatalytic activity of p-3DG and the ability of nanochannels to enrich TCP through hydrogen bonding, the VMSF/p-3DG sensor can sensitively detect TCP in the range of 10 nM to 0.1 μM and 0.1–15 μM with a low limit of detection (LOD) of 2.4 nM. Compared with p-3DG and VMSF-modified 2D electrodes, the fabricated sensor has a wide detection linear range and low LOD. The coexistence of model interferents such as protein, surfactant, and humic acid did not affect the electrochemical response of TCP, confirming the high anti-fouling ability of the VMSF/p-3DG sensor. In addition, prochloraz in vegetable and fruit samples was indirectly determined because TCP was the metabolite of prochloraz.
Performance evaluation of compound parabolic solar collector using different nanofluids: An experimental study
Authors: Farhan Muhammad , Shahid Muhammad Ihsan , Jamil Furqan , Usman Muhammad , Mujtaba M. A. , Saleem Muhammad Wajid , Nasir Shah Syed , Saghir Shahid , Yusuf Abdulfatah Abdu , Mousa Mohamed , El-Shafay A.S. 
Published: September 2022

The article reports an experimental study on a non-tracking compound parabolic collector (CPC) with nanofluid and hybrid nanofluids (NFs). An experimental setup was fabricated having a concentration ratio of 4.17, 0.828 m2 collector area, 24° of half acceptance angle, and an evacuated tube receiver having 1.85 m length. Fluids like water and NFs have been investigated in CPC performance improvement, but current research deals with NFs and hybrid NFs in a CPC as rare studies are found on a CPC using NFs. The 0.010 and 0.015 wt% concentration were used of nanofluids (NFs) which were silica/water + ethylene glycol (SiO2)/(H2O + EG), and hybrid nanofluids of magnesium oxide + carbon black/water (MgO + CB/H2O) and carbon black + graphene nanoplatelets/water (CB + GNPs/H2O) at flow rates of 0.020, 0.015, and 0.010 kg/s. The experimentation was performed under real climate conditions of Taxila, Pakistan, and solar irradiance and ambient temperature were measured to determine the performance of the CPC in comparison to a simple base fluid which was water. The experimental results revealed that a maximum temperature difference of 8.5°C with an around thermal efficiency of 38.51% was achieved for hybrid pair of MgO + CB at a flow rate of 0.010 kg/s and volumetric concentration of 0.015 wt%. The efficiency variation using NFs (SiO2/EG + H2O) varies from 12.8% to 59.1% from lowest 0.010 kg/s to highest 0.020 kg/s flow rates, and volumetric concentrations (0.010 and 0.015 wt%) of nanoparticles. For similar experimental conditions, efficiency variation for (CB + GNPs) pair varies from 14.2% to 65.6% for aforementioned conditions. Efficiency variation from 15.3% to 66.3% was attained using MgO + CB in the base fluid of water for said flow rates and volumetric concentrations of nanoparticles. In addition, an efficiency enhancement of 24.3%, 30.8%, and 31.5% was observed for SiO2/EG + H2O, CB + GNPs/H2O, and MgO + CB/H2O, respectively, at maximum flow rates and volumetric concentration of nanoparticles as compared to water. Therefore, the usage of hybrid-based NF in the CPC is beneficial in terms of efficiency enhancement, and it will bring young research workers to get deep into this field to bring revolution in the area of solar energy.
A ferroptosis associated gene signature for predicting prognosis and immune responses in patients with colorectal carcinoma
Authors: Yan Lijun , Chen Xi , Bian Zhaolian , Gu Chunyan , Ji Hanzhen , Chen Liyan , Xu Haifeng , Tang Qiyun 
Published: September 2022

Background: Colorectal carcinoma (CRC) is one of the most prevalent malignancies globally. Ferroptosis, a novel type of cell death, is critical in the development and treatment of tumors.Objective: This study was designed to establish a genetic signature for ferroptosis which has a predictive effect on the outcomes and immunotherapeutic response of CRC.Methods: Data of CRC patients were retrieved from TCGA and GEO databases. The genes associated with ferroptosis were obtained from GeneCards. The genetic signature for ferroptosis was identified by performing Cox regression analysis. Kaplan–Meier and ROC analysis were performed to assess the prognosis role of the genetic signature. CIBERSORT tool was used to identify a potential association of the genetic signature with the immune cells. The potential immunotherapeutic signatures and drug sensitivity prediction targeting this signature were also discussed. Immunohistochemistry was used to detect expression of ferroptosis-associated genes in CRC tissues and adjacent tissues.Results: A ferroptosis-associated gene signature comprised of three genes (CDKN2A, FDFT1, and ACSL6) was developed for prediction of prognosis and evaluation of immune responses in CRC. Patients in the high-risk group tended to have a poor prognosis. In CRC, the ferroptosis-associated gene signature may function as independent predictors. Additionally, the expressional levels of the immune checkpoint proteins PD-L1 and CTLA-4 were substantially increased in the high-risk group. Moreover, we can distinguish between patients based on their immunotherapeutic responses more effectively if we categorize them by this signature. Additionally, candidate compounds were identified for the differentiation of CRC subtypes.Conclusion: The ferroptosis-associated gene signature identified in this study is effective in predicting the prognosis and evaluating immunotherapeutic response in CRC patients, and provides us with novel insights into the potential effect of ferroptosis targeted treatment on CRC.
Pan-cancer analysis reveals interleukin-17 family members as biomarkers in the prediction for immune checkpoint inhibitor curative effect
Authors: Han Xiaying , Ye Jianxin , Huang Runzhi , Li Yongai , Liu Jianpeng , Meng Tong , Song Dianwen 
Published: September 2022

BackgroundThe interleukin-17 (IL-17) family contains six homologous genes, IL-17A to IL-17F. Growing evidence indicates that dysregulated IL-17 family members act as major pathogenic factors in the early and late stages of cancer development and progression. However, the prevalence and predictive value of IL-17 for immune checkpoint inhibitor (ICI) therapeutic effectiveness in multiple tumor types remain largely unknown, and the associations between its expression levels and immunotherapy-associated signatures also need to be explored.MethodsThe pan-cancer dataset in The Cancer Genome Atlas (TCGA) was downloaded from UCSC Xena (http://xena.ucsc.edu/). The immunotherapeutic cohorts included IMvigor210, which were obtained from the Gene Expression Omnibus database and included in a previously published study. Other datasets, namely, the GEO dataset and PRECOG, GEO, and METABRIC databases, were also included. In 33 TCGA tumor types, a pan-cancer analysis was carried out including their expression map, clinical risk assessment, and immune subtype analysis, along with their association with the stemness indices, tumor microenvironment (TME) in pan-cancer, immune infiltration analysis, ICI-related immune indicators, and drug sensitivity. RT-PCR was also carried out to verify the gene expression levels among MCF-10A and MCF-7 cell lines.ResultsThe expression of the IL-17 family is different between tumor and normal tissue in most cancers, and consistency has been observed between gene activity and gene expression. RT-PCR results show that the expression differences in the IL-17 family of human cell (MCF-10A and MCF-7) are consistent with the bioinformatics differential expression analysis. Moreover, the expression of the IL-17 family can be a sign of patients’ survival prognosis in some tumors and varies in different immune subtypes. Moreover, the expression of the IL-17 family presents a robust correlation with immune cell infiltration, ICI-related immune indicators, and drug sensitivity. High expression of the IL-17 family is significantly related to immune-relevant pathways, and the low expression of IL-17B means a better immunotherapeutic response in BLCA.ConclusionCollectively, IL-17 family members may act as biomarkers in predicting the prognosis of the tumor and the therapeutic effects of ICIs, which provides new guidance for cancer treatment.
Immune depletion of the methylated phenotype of colon cancer is closely related to resistance to immune checkpoint inhibitors
Authors: Zhong Chengqian , Xie Tingjiang , Chen Long , Zhong Xuejing , Li Xinjing , Cai Xiumei , Chen Kaihong , Lan Shiqian 
Published: September 2022

BackgroundMolecular typing based on single omics data has its limitations and requires effective integration of multiple omics data for tumor typing of colorectal cancer (CRC).MethodsTranscriptome expression, DNA methylation, somatic mutation, clinicopathological information, and copy number variation were retrieved from TCGA, UCSC Xena, cBioPortal, FireBrowse, or GEO. After pre-processing and calculating the clustering prediction index (CPI) with gap statistics, integrative clustering analysis was conducted via MOVICS. The tumor microenvironment (TME) was deconvolved using several algorithms such as GSVA, MCPcounter, ESTIMATE, and PCA. The metabolism-relevant pathways were extracted through ssGSEA. Differential analysis was based on limma and enrichment analysis was carried out by Enrichr. DNA methylation and transcriptome expression were integrated via ELMER. Finally, nearest template or hemotherapeutic sensitivity prediction was conducted using NTP or pRRophetic.ResultsThree molecular subtypes (CS1, CS2, and CS3) were recognized by integrating transcriptome, DNA methylation, and driver mutations. CRC patients in CS3 had the most favorable prognosis. A total of 90 differentially mutated genes among the three CSs were obtained, and CS3 displayed the highest tumor mutation burden (TMB), while significant instability across the entire chromosome was observed in the CS2 group. A total of 30 upregulated mRNAs served as classifiers were identified and the similar diversity in clinical outcomes of CS3 was validated in four external datasets. The heterogeneity in the TME and metabolism-related pathways were also observed in the three CSs. Furthermore, we found CS2 tended to loss methylations while CS3 tended to gain methylations. Univariate and multivariate Cox regression revealed that the subtypes were independent prognostic factors. For the drug sensitivity analysis, we found patients in CS2 were more sensitive to ABT.263, NSC.87877, BIRB.0796, and PAC.1. By Integrating with the DNA mutation and RNA expression in CS3, we identified that SOX9, a specific marker of CS3, was higher in the tumor than tumor adjacent by IHC in the in-house cohort and public cohort.ConclusionThe molecular subtypes based on integrated multi-omics uncovered new insights into the prognosis, mechanisms, and clinical therapeutic targets for CRC.
Dexamethasone reduces vascular endothelial growth factor in comparison to placebo in post-operative chronic subdural hematoma samples: A target for future drug therapy?
Authors: Edlmann Ellie , Giorgi-Coll Susan , Thelin Eric P. , Hutchinson Peter J. , Carpenter Keri L. H. 
Published: September 2022

BackgroundChronic subdural hematoma (CSDH) is a collection of blood and fluid that arises on the brain surface due to a combination of trauma and/or inflammation. The mainstay of treatment is surgical drainage, but CSDH can recur. Dexamethasone has been shown to reduce CSDH recurrence, but its mechanism of action has not been fully elucidated. Understanding the inflammatory mediators driving CSDH formation and recurrence and how dexamethasone alters this can help develop new therapeutic strategies.MethodsA subgroup of adult patients recruited to the Dex-CSDH trial, randomized to dexamethasone or placebo, who had surgery for their CSDH, were included. CSDH fluid and peripheral blood were collected intraoperatively, from post-operative drains and operated recurrences. Samples were analyzed using a 12-plex panel of inflammatory mediators. Clinical patient data were also reviewed.ResultsA total of 52 patients, with a mean age of 76 years, were included. Five recurrent CSDHs occurred. Vascular endothelial growth factor (VEGF) had the highest concentration across all CSDHs, and only matrix metalloproteinase (MMP)-9 had lower concentrations in CSDH compared to plasma but was increased in recurrent CSDHs. The interleukin (IL)-10 concentration was significantly lower in primary CSDHs that recurred. Most inflammatory mediators increased post-operatively, and dexamethasone significantly reduced the post-operative peak in VEGF on day 2, compared to placebo.ConclusionIt is evident that VEGF plays a critical role in the inflammatory response in CSDH. The post-operative reduction with dexamethasone could signal the mechanism by which it reduces recurrence. Novel therapies with a better side-effect profile than dexamethasone should be targeted at VEGF or potential alternatives such as IL-10 supplementation.
Determinants of functioning and health-related quality of life after vestibular stroke
Authors: Schuhbeck Franziska , Strobl Ralf , Conrad Julian , Möhwald Ken , Jaufenthaler Patricia , Jahn Klaus , Dieterich Marianne , Grill Eva , Zwergal Andreas 
Published: September 2022

BackgroundStroke accounts for 5–10% of all presentations with acute vertigo and dizziness. The objective of the current study was to examine determinants of long-term functioning and health-related quality of life (HRQoL) in a patient cohort with vestibular stroke.MethodsThirty-six patients (mean age: 66.1 years, 39% female) with an MRI-proven vestibular stroke were followed prospectively (mean time: 30.2 months) in the context of the EMVERT (EMergency VERTigo) cohort study at the Ludwig-Maximilians Universität, Munich. The following scores were obtained once in the acute stage (&lt;24 h of symptom onset) and once during long-term follow-up (preferably &gt;1 year after stroke): European Quality of Life Scale-five dimensions-five levels questionnaire (EQ-5D-5L) and Visual Analog Scale (EQ-VAS) for HRQoL, Dizziness Handicap Inventory (DHI) for symptom severity, and modified Rankin Scale (mRS) for general functioning and disability. Anxiety state and trait were evaluated by STAI-S/STAI-T, and depression was evaluated by the Patient Health Questionnaire-9 (PHQ-9). Voxel-based lesion mapping was applied in normalized MRIs to analyze stroke volume and localization. Multiple linear regression models were calculated to determine predictors of functional outcome (DHI, EQ-VAS at follow-up).ResultsMean DHI scores improved significantly from 45.0 in the acute stage to 18.1 at follow-up (p &lt; 0.001), and mean mRS improved from 2.1 to 1.1 (p &lt; 0.001). Mean HRQoL (EQ-5D-5L index/EQ-VAS) changed from 0.69/58.8 to 0.83/65.2 (p = 0.01/p = 0.11). Multiple linear regression models identified higher scores of STAI-T and DHI at the time of acute vestibular stroke and larger stroke volume as significant predictors for higher DHI at follow-up assessment. The effect of STAI-T was additionally enhanced in women. There was a significant effect of patient age on EQ-VAS, but not DHI during follow-up.ConclusionThe average functional outcome of strokes with the chief complaint of vertigo and dizziness is favorable. The most relevant predictors for individual outcomes are the personal anxiety trait (especially in combination with the female sex), the initial symptom intensity, and lesion volume. These factors should be considered for therapeutic decisions both in the acute stage of stroke and during subsequent rehabilitation.
Tumor imaging and photothermal therapy in second near infrared window: A systematic review and meta-analysis
Authors: Fan Fuhan , Hou Ya , Zhang Yating , Zeng Yong , Zhang Yi , Zhang Sanyin , Meng Xianli , Wang Xiaobo 
Published: September 2022

BackgroundSecond near-infrared window (NIR-II, 1000-1700 nm) technology for tumor imaging and photothermal therapy (PTT) is an innovative method for tumor diagnosis and treatment. The NIR-II probe can specifically identify tumor cells, and effectively convert light energy into heat energy under the irradiation of NIR laser, thus achieving the integration of non-invasive tumor diagnosis and treatment. In the present study, we conducted a systematic review and meta-analysis of preclinical investigations to corroborate the efficacy and safety of photothermal therapy.MethodsRelevant preclinical data were retrieved by searching PubMed, Web of Science, CNKI, WANFANG and VIP information databases. And the acquired data were analyzed by RevMan Version 5.3 software.ResultsAccording to the inclusion criteria, forty-two articles relating to NIR-II tumor imaging and PTT were recruited for further in-depth analysis. The NIR-II photoacoustic and fluorescence imaging could quickly and accurately identify tumor in mice, manifesting higher signal intensity on tumor site than that of normal tissue. After PTT, the tumor volume of mice decreased miraculously [RR=8.49, 95%CI (4.64, 15.55), P&lt;0.00001], and even disappeared completely [RR=7.01, 95%CI (3.04, 16.13), P&lt;0.00001] with no potential risk of affecting the blood routine.ConclusionsPTT guided by NIR-II imaging can effectively diagnose the tumor lesion and eliminate it with the advantages of non-invasive and higher biosafety.
Massive gastrointestinal haemorrhage caused by pancreatic pseudocyst complicated with Dieulafoy's disease in a child: A case report and review of the literature
Authors: Liu Lintao , Zhang Lichao , Zhu Xiaoli , Li Meng , Cao Juan , Ji Likang , Qi Xiaoyang , Xu Weili 
Published: September 2022

BackgroundPancreatic pseudocyst (PPC) with massive gastrointestinal bleeding is rare, especially in children. Inadvertent intraoperative examination and damage to the gastric mucosa and malformed blood vessels by the fluid content of PPC can lead to massive bleeding, which may endanger the patient's life.Case presentationHere, we present a case of an 8-year-old boy who was diagnosed with a massive gastrointestinal haemorrhage caused by PPC complicated with Dieulafoy's disease. At his first admission, his complaint was being hit to the stomach by the handlebar while riding bicycle 24 h before admission. After being hospitalized, he was diagnosed with pancreatic injury by abdominal CT. Conservative treatment lasted for 1 month in the Department of Pediatric Surgery. Then, a pancreatic pseudocyst was formed. Under the guidance of ultrasonic endoscopy, cyst puncture and drainage of pseudocysts through the gastric wall were performed. Unexplained hematemesis occurred 8 days after surgery. Emergency gastroscopy was performed, and abnormal submucosal vascular haemorrhage was found at the gastric fundus. Gastric Dieulafoy's disease was diagnosed. The boy underwent gastroscopic titanium clipping of abnormal arteries. He had no complications during the 3-month follow-up. Then, the patient returned to the hospital, and the stent was removed under endoscopy. No bleeding was found, and the patient was discharged. The patient recovered smoothly and was followed up for half a year without any complications, and hematological indicators were normal.ConclusionEndoscopic ultrasonography-guided gastric puncture and internal drainage of cysts is a safe and effective surgical method for the treatment of pancreatic pseudocysts. However, at the same time, it is necessary to thoroughly and carefully explore the stomach cavity to prevent adverse consequences caused by a missed diagnosis of gastric Dieulafoy's disease or other abnormal abnormalities.