bluuebunny/crossref_metadata_2025_split huggingface dataset:
Point of Care Ultrasound to Identify Rib Osteomyelitis in the Infants. A Case Report.
Authors: Montero-Yéboles Raúl , Manzanares-Santos Silvia , Diaz-Rueda Laura , Velasco-Jabalquinto Maria José , Sáez Beatriz Ruiz- , Pérez-Navero Juan luis 
Published: March 2022

Abstract
SUMMARY The number of reported cases of rib osteomyelitis are very infrequent. Magnetic resonance is generally the best test in these cases, due to its high sensitivity and specificity. However, given this technique has some drawbacks, it is usually restricted to cases of bad evolution. A relevant, easy to perform, bedside imaging test is the point of care ultrasound. CLINICAL CASE We present the case of a two-month-old infant with a rib osteomyelitis detected by point of care ultrasound in the context of sepsis after abdominal surgery. The thoracic ultrasound revealed a lesion adjacent to the right sixth rib and round extraparenchymal collection in the right costophrenic sinus. Chest CT confirmed the presence of a hypodense collection at the costochondral junction of the right seventh rib with pleural thickening and an increase in soft tissues, compatible with osteomyelitis. Our ultrasound finding in the absence of specific clinical data of rib osteomyelitis led us to a change in the duration of the antibiotic treatment already established and therefore likely led to an improvement in the patient's prognosis. CONCLUSION The ultrasound has gained increasing prominence due to its usefulness in diagnosis and monitoring, especially in pediatric intensive care, given the ease of use at the patient's bedside.
On the Injectivity of an Integral Operator Connected to Riemann Hypothesis
Authors: Adam Dumitru 
Published: March 2022

Abstract
The equivalent formulation of the Riemann Hypothesis (RH) given by Alcantara-Bode ([1], 1993) is outstanding, reducing one of the most important unsolved problems in pure mathematics to a problem whose investigation could be made by standard techniques of applied mathematics: RH holds if and only if the integral operator on the Hilbert space L2(0,1) having the kernel function defined by the fractional part of (y/x), is injective.The method introduced in this paper to deal with, is based on the main result obtained: a linear bounded Hermitian operator strict positive on a dense family of subspaces, having the sequence of the inverse condition numbers bounded inferior by a strict positive constant, is injective.Using its version for integral operators on L2(0,1) by choosing a dense family of approximation subspaces well known in literature, we proved the injectivity of the integral operator from the equivalent formulation of the RH given in [1].
Conserved RNA Secondary Structure In Cherry Virus A 5′-UTR Associated With Translation Regulation
Authors: Wang Deya , Yang Chen , Deng Yanmei , Cao Xue , Xu Wei , Han Zishuo , Li Qingliang , Yang Yang , Yuan Xuefeng 
Published: March 2022

Abstract
Background: A variety of cis-acting RNA elements with structures in the 5′- or 3′-untranslated region (UTR) of viral genomes play key roles in viral translation. Cherry virus A (CVA) is a member of the genus Capillovirus in the family Betaflexiviridae. It has a positive single-stranded RNA genome of ~7400 nucleotides (nt). The length of the CVA 5′-UTR is ~100 nt; however, the function of this long UTR has not yet been reported.Methods: Molecular and phylogenetic analyses were performed on 75 CVA sequences, which could be divided into four groups, and the RNA secondary structure was predicted in four CVA 5-′UTR types. These four CVA 5′-UTR types were then inserted upstream of the firefly luciferase reporter gene FLuc (FLuc), and in vitro translation of the corresponding transcripts was evaluated using wheat germ extract (WGE). Then, in-line structure probing was performed to reveal the conserved RNA structures in CVA -5′UTR.Results: The four CVA 5′-UTR types appeared to have a conserved RNA structure, and the FLuc construct containing these four CVA 5′-UTR types increased the translation of FLuc by 2-3 folds, suggesting weak translation enhancement activity. Mutations in CVA 5′-UTR that suppressed translation, suggesting that the conserved RNA structure was important for function.Conclusion: The conserved RNA secondary structure was identified by structural evolution analysis of different CVA isolates and was found to regulate translation.
Nutritional Quality of Bovine Cheeks Meal From Tannery Industry as a Protein Source for Monogastric Animals
Authors: Castro-Bedriñana Jorge Isaac , Chirinos-Peinado Doris , Lizárraga-Velásquez Flor , Ríos-Ríos Elva , Quispe-Ramos Rolando 
Published: March 2022

Abstract
The digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and the concentration of digestible energy (DE) and metabolizable energy (ME) of bovine cheek meal (CM), included in 15 and 20% were compared. The bovine cheeks not chemically contaminated, prior to the pre-tanning stage of the hides, were collected, washed, cooked, dried, and ground for their nutritional evaluation. As the CM cannot constitute 100% of the guinea pig diet, to estimate its digestibility the indigestibility coefficients of a reference diet (Barley meal-BM) were used and assuming that the indigestibility of the BM is the same in the diets with 15% and 20% CM the digestibility of CM was estimated. Twelve six-month-old male guinea pigs were used, divided into three groups of four animals per group, housed in individual metabolic cages for the total collection of urine-free feces. The DM, CP and EE digestibility’s of CM-15% and CM-20% were 72.30, 95.71 and 78.34%, and 76.34, 96.03 and 82.37%, and the DE and ME contents were 4089.5, 3353.8 and 4129.0 and 3385.8 (P < 0.05). The results would indicate that the production of CM would be a viable option to obtain a protein meal of high nutritional value for feeding guinea pigs and could totally replace other less available and expensive protein sources, such as fish meal or soybean meal, contributing to sustainable animal production and with environmental decontamination strategies.
The effect of propolis on 5-fluorouracil-induced cardiac toxicity in rats
Authors: Barary Mohammad , Hosseinzadeh Rezvan , Liang Jackson J. , Mansouri Razieh , Sio Terence T. , Hosseini Mohammad , Kazemi Sohrab , Moghadamnia Ali Akbar 
Published: March 2022

Abstract
Background
5- Fluorouracil (5-FU) is one of the most common chemotherapeutic agents used in treating solid tumors, and the 5-FU-induced cardiotoxicity is the second cause of cardiotoxicity induced by chemotherapeutic drugs. Propolis (Pro) has vigorous anti-inflammatory activity. Its cardio-protective characteristic against doxorubicin-induced cardiotoxicity was previously proven. The current study aimed to appraise the effect of Pro on 5-FU-induced cardiotoxicity in rats.
Methods
Twenty-four male Wistar rats were divided into four groups: Control, 5-FU, 5-FU + Pro 250 mg/kg, and 5-FU + Colchicine (CLC) 5 mg/kg. Different hematological, serological, biochemical, histopathological, and molecular assays were performed to assess the study aim. Moreover, rat myocardium (H9C2(2 − 1)) and human skin fibroblast (HNFF-P18) cell lines were also used to assess this protective effect in-vitro.
Results
5-FU resulted in significant cardiotoxicity represented by an increase in malondialdehyde (MDA) levels, cyclooxygenase-2 (COX-2), and tumor necrosis factor-α (TNF-α) expression, cardiac enzyme levels, and histopathological degenerations. 5-FU treatment also decreased bodyweight, total anti-oxidant capacity (TAC), catalase (CAT) levels, blood cell counts, and hemoglobin (Hb) levels. In addition, 5-FU disrupted ECG parameters, including increased elevation in the ST-segment and increased QRS complex and QTc duration. Treating with Pro reduced oxidative stress, cardiac enzymes, histopathological degenerations, and COX-2 expression in cardiac tissue alleviated ECG disturbances and increased the number of blood cells and TAC levels. Moreover, 5-FU-induced bodyweight loss was ameliorated after treatment with Pro.
Conclusion
Our results demonstrated that treatment with Pro significantly improved cardiotoxicity induced by 5-FU in rats.
The Occupational Neck Pain Prevention Behaviors Assessment Questionnaire: Development and psychometric evaluation
Authors: Tavafian Sedigheh Sadat , Moradi Zohreh , Kazemi Seyedeh Somayeh , Montazeri Ali 
Published: March 2022

Abstract
Background: Neck pain is a common problem among teachers. This study aimed to develop and evaluate an instrument for measuring occupational neck pain preventive behaviors among teachers. Method: In order to develop a neck pain preventive behavior this study was conducted in December 2020 in two phases: qualitative, and quantitative. In qualitative phase a purposeful sample of teachers were interviewed to generate an item pool. Then content validity and face validity were performed to provide a provisional version of the questionnaire. In the next stage, to evaluate the psychometric properties of the questionnaire, a cross sectional study was conducted. In total, 146 teachers participated in this study (with a mean age 36.7; SD 8.92 years). The questionnaire was distributed among a sample of teachers. Exploratory factor analysis was used to obtain the factor structure of the questionnaire. The item-scale correlation matrix was examined to further evaluate the validity of the questionnaire. Internal consistency (Cronbach's alpha) was calculated to assess reliability and intra class correlation coefficient (ICC) to assess stability.Results: Based on analysis of the exploratory factor, 8 factors with 43 substances, that together accounted for 65,25% variances were obtained. Also, the correlation matrix in the case scale to establish the validity of the questionnaire showed satisfactory results. The results of face validity showed that 4 factors were not approved and were removed from the questionnaire. Reliability evaluation with internal consistency method (Cronbach's alpha) showed excellent compatibility (0.87). The intra class correlation reliability assessment showed that the questionnaire has satisfactory stability (ICC) (0.92).Conclusion: This study provides the reliability and validity for the Occupational Pain Neck Preventive Behaviors Questionnaire. The questionnaire evaluates occupational neck pain prevention behaviors among teachers and possibly is useful for using in other settings.
lncRNA TERC promotes gastric cancer cell proliferation, migration and invasion by targeting miR-423-5p to regulate SOX12 expression
Authors: Huang Xiaoyan , Jin Zhankui , He Yangzhi , Liu Xiao , Duan Xianglong , Xu Cuixiang , Wang Jianhua 
Published: March 2022

Abstract
Background
Increasing evidences demonstrate long non-coding RNAs (lncRNAs) play an critical roles in gastric cancer initiation progression. However, the biological function of long non-coding RNA (lncRNA) telomerase RNA component (TERC) remains unknown in human gastric cancer. The present study aimed to determine the biological function and underlying molecular mechanism of lncRNA TERC in gastric cancer progression.
Methods
The expression levels of lncRNA TERC in gastric cancer tissues and cell lines were anaylzed using reverse transcription-quantitative PCR. The effects of lncRNA TERC on the proliferation, migration and invasion of gastric cancer cells were determined using Cell Counting Kit-8 and Transwell assays, respectively. Dual luciferase reporter and argonaute 2-RNA immunoprecipitation assays were used to detect the binding between lncRNA TERC and microRNA (miR)-423-5p. Western blotting was performed to measure the expression levels of SOX12, N-cadherin, E-cadherin, MMP9 and proliferating cell nuclear antigen (PCNA).
Results
lncRNA TERC expression levels were upregulated in gastric cancer cells and tissues, while miR-423-5p expression was downregulated. The upregulation of lncRNA TERC was associated with a shorter overall survival in patients with gastric cancer. lncRNA TERC knockdown significantly reduced the proliferation, migration and invasion of HGC-27 and SNU-1 cells. Furthermore, lncRNA TERC knockdown in HGC-27 and SNU-1 cells significantly downregulated the expression levels of SOX12, N-cadherin, MMP9 and PCNA, and upregulated the expression levels of miR-423-5p and E-cadherin. miR-423-5p was also identified as a target of lncRNA TERC and was found to directly bind with lncRNA TERC. Moreover, miR-423-5p was discovered to directly target SOX12 to inhibit the proliferation, migration and invasion of HGC-27 and SNU-1 cells.
Conclusion
The findings of the current study suggested that lncRNA TERC may regulate the miR-423-5p/SOX12 signaling axis via directly sponging miR-423-5p and inhibiting SOX12 expression, thereby leading to the progression of gastric cancer. These findings may offer novel targets for future gastric cancer therapy.
Global Carbon Inequality, 1990-2019: The Impact of Wealth Concentration on the Distribution of World Emissions
Authors: Chancel Lucas 
Published: March 2022

Abstract
This paper estimates the global inequality of individual greenhouse gas (GHG) emissions between 1990 and 2019, using a newly assembled dataset of income and wealth inequality and Environmental Input-Output tables. I find that the bottom half of the world population emits 12% of global emissions, while the top 10% emits 48% of the total. The global top 1% share in world emissions rose from 14% in 1990 to 17% in 2019. While two thirds of the inequality in individual emissions was due to inequalities between countries in 1990, the situation has entirely reversed: in 2019, 63% of the global inequality in individual emissions was due to gaps between low and high emitters within countries. Emissions from investments, rather than from consumption, represent the bulk of emissions of the rich: around 70% of global top 1% emissions come from their investments. This has major implications for contemporary debates on fair climate policies. I stress the need for more systematic data on individual carbon emissions to allow informed policy debates.
A single amino acid at PNT domain of ERG mediates its leukemogenic activity through interaction with the NCoR-HDAC3 co-repressor complex
Authors: Kugler Eitan , Madiwale Shreyas , Yong Darren , Birger Yehudit , Thoms Julie , Sykes David , Yassin Muhammad , Aqaqe Nasma , Rein Avigail , Fishman Hila , Geron Ifat , Chen Chun-Wei , Raught Brian , Liu Qiao , Milyavsky Michael , Pimanda John , Privé Gilbert , Izraeli Shai 
Published: March 2022

Abstract
The ETS transcription factor ERG is involved in several cancers including leukemias. We and others have demonstrated a direct role for ERG in leukemia initiation and maintenance of several subtypes of myeloid and lymphoid leukemias. However, ERG domains and co-factors involved in leukemogenesis remains largely uncharacterized and as a transcription factor it is undruggable. Here we report a critical role for the conserved amino-acid proline at position 199, at the 3’ end of the PNT domain, for ERG’s leukemogenic activity. Specifically, we demonstrate that it is required for ERG-induced self-renewal and restriction of myeloid differentiation in hematopoietic progenitor cells and for initiation of leukemia in mouse transduction/transplantation models. Mechanistically, we show that P199 facilitates the interaction of ERG with the NCoR-HDAC3 co-repressor complex. Inhibition of HDAC3 reverses ERG’s restriction of myeloid differentiation and reduces human ERG-dependent leukemic burden in immunodeficient mice. Thus, the interaction of ERG with the NCoR-HDAC3 co-repressor complex is required for its oncogenic activity and modulation of this interaction may provide an opportunity for therapeutic intervention.
Nicotiana Tabacum Tolerance to CPSMV Involves Increased H2O2/phenol Contents and Adjustments of Defense Protein Biosynthesis and Expression of Initiation/elongation Factors
Authors: Bret Raissa , Lima-Melo Yugo , Carvalho Cristina , Oliveira Jose Tadeu Abreu 
Published: March 2022

Abstract
CPSMV is one of the various pathogens that infect cowpea (Vigna unguiculata L. Walp.), an important leguminous crop, and cause significant yield losses. To the best of our knowledge, there is no report on the reaction of tobacco, a model, non-leguminous plant, in response to CPSMV. In this study, we inoculated Nicotiana tabacum L. (var. Xanthi) leaves with a CPSMV isolate to evaluate which type of relationship (compatible, incompatible or tolerance) is established in this pathosystem and what are the plant biochemical responses to the CPSMV challenge. Tobacco plants were grown in vitro under controlled conditions and their leaves inoculated with CPSMV. The disease symptoms, the presence of the virus, and the tobacco biochemical responses were assessed in comparison with the mock-inoculated controls at 1, 2, and 6 days after inoculation (DAI). CPSMVCE modulated the activity of some enzymes and gene expression in tobacco to its own benefit, whereas, in parallel, N. tabacum actively responds against the viral infection. Accordingly, the severe disease symptoms caused by CPSMVCE in susceptible cowpea genotypes were not developed although the CPSMVCE viral particles were present in the tobacco leaves at 6 DAI. These findings suggest that N. tabacum (var. Xanthi) exhibited the patterns of a typical tolerant host to CPSMVCE. However, a comprehensive understanding of the mechanisms of tolerance of N. tabacum (var. Xanthi) to CPSMVCE is of utmost importance to develop new cowpea genotypes tolerant or resistant to this viral disease.
Cyclosaplin analog peptides design and analysis of their cytotoxicity effects on breast cancer cell line (MDA-MB-231) and human leukemia cell line (K562)
Authors: Elyaderani Parisa Kadkhodaei , Abbasi Elham , Asgharian Ali Mohammad 
Published: March 2022

Abstract
Objective: It was the aim of this study to develop Cyclosaplin analogs and assess the anticancer effects of those analog peptides on MDA-Mb-231 as well as K562 cell lines. The analogs of Cyclosaplin peptide (Cyclosaplin-2A and Cyclosaplin-7G) were designed and then investigated by online web server predictor AntiCP. The analog peptides were applied to MDA-MB-231 and K562 cells in various concentrations and for various periods of time. The anticancer potential was confirmed by the MTT assay. Hemolytic activity also was assessed. In order to investigate the apoptotic effects of peptides on cancer cells, various tests such as morphological examination, Giemsa test, and DNA fragmentation were performed. Lactate dehydrogenase leakage also was examined to confirm the effects of analogs. Results: Our experimental and computational data show that the analog peptides have anticancer potential. The MTT assay and morphological study confirmed the anticancer effects. Other tests also confirmed the anticancer effect of the analog peptides. According to hemolytic assays, none of the analog peptides possess any hemolytic activity against human erythrocytes, indicating the compounds are not toxic for normal cells. Analog peptides found in this study were shown to have anticancer potential on two human cancer cell lines.
B7-H6 is a Prognostic Biomarker for Human Renal Clear Cell Carcinoma and Correlates with Immune Cell Infiltration
Authors: Sun Ziling , Qiu Hao , Gao Shangshang , Wang Jiamin 
Published: March 2022

Abstract
The B7 homolog 6 (B7-H6) is a newly discovered co-stimulatory molecule of the B7 family, which found to be widely expressed in various tumors. The purpose of this study is to identify the prognostic significance of B7-H6 and its relation with immune cell infiltration in human clear cell renal cell carcinoma (ccRCC). Using The Cancer Genome Atlas (TCGA) database, Gene Expression Profiling Interactive Analysis (GEPIA), Tumor Immune Estimation Resource (TIMER) database and Kaplan-Meier plotter database, we explored B7-H6 expression and its prognostic significance of ccRCC. Finally, the association between B7-H6 expression and tumor infiltration immune status was estimated via TIMER and CIBERSORT. We found that B7-H6 was remarkably elevated in ccRCC than normal kidney tissues and adjacent tumor-free tissues. Patients with high B7-H6 expression had favorable overall, disease-free survival and progress-free interval. Univariate and multivariate Cox analyses demonstrated that B7-H6 expression was an independent protective factor for overall survival in ccRCC patients. Moreover, the expression of B7-H6 was correlated with differential immune cell infiltration and various immune biomarkers. This study reveals that increased B7-H6 expression correlates with favorable prognosis in ccRCC. It could be a novel clinical biomarker and provide new insights for future treatments in ccRCC.
Hypertensive crisis merging paroxysmal supraventricular tachycardia after dezocine injection during caesarean delivery: a case report
Authors: Huang Liping , Yan Xi , Yi Mingliang 
Published: March 2022

Abstract
Background: Dezocine is a novel opioid receptor-antagonistic analgesic, which is widely used in clinic work. However, its usage in cesarean section is rare.Case presentation: A 28-year-old woman of 38 weeks' gestation (163cm, 79kg) underwent emergency cesarean section because of pelvic outlet stenosis in November 2021. There was no history of preeclampsia, anticoagulation, or hemorrhagic diathesis. The most recent electrocardiogram (ECG) during pregnancy was sinus tachycardia, and the heart rate (HR) was 108 beats/min. After the accomplishment of spinal anesthesia, the cesarean section started smoothly. After the fetus was delivered, the patient complained of pain when suturing the skin. The anesthesiologist gave her dezocine 5mg and the parturient presented with hypertensive crisis merging paroxysmal supraventricular tachycardia (PSVT). The fastest HR was 178 beats/min and the highest blood pressure (BP) was 190/128mmHg. After Esmolol 20mg and Urapidil 12.5mg were administrated, her HR and BP returned normal range 3min later. After observation, the patient returned to the ward one hour later and left hospital after 5 days. The patient denied the history of PSVT and hyperthyroidism.Conclusion: Nowadays，dizocine has been widely used in the area of postoperative analgesia. However, its usage is rare in obstetrics and gynecology. The malignant adverse reactions of dizocine needs to be highly vigilant.