bluuebunny/crossref_metadata_2025_split huggingface dataset:
IL-1β turnover by TRIP12 and AREL1 ubiquitin ligases and UBE2L3 limits inflammation
Authors: Mishra Vishwas , Crespo-Puig Anna , McCarthy Callum , Masonou Tereza , Glegola-Madejska Izabela , Dejoux Alice , Dow Gabriella , Eldridge Matthew J. G. , Marinelli Luciano H. , Meng Meihan , Wang Shijie , Bennison Daniel J. , Shenoy Avinash R. 
Published: September 2022

ABSTRACTThe cytokine interleukin-1β (IL-1β) has pivotal roles in antimicrobial immunity, but also incites inflammatory pathology. Bioactive IL-1β is released following proteolytic maturation of the pro-IL-1β precursor by caspase-1 inflammasomes. UBE2L3/UBCH7, a conserved ubiquitin conjugating enzyme, promotes pro-IL-1β ubiquitylation and proteasomal disposal. However, UBE2L3 actionsin vivoand ubiquitin ligases involved in this process are unknown. Here we report that deletion ofUbe2l3in mice markedly reduces pro-IL-1β turnover in macrophages, leading to excessive mature IL-1β production, neutrophilic inflammation and disease symptoms following inflammasome activation. A family-wide siRNA screen identified two ubiquitin ligases, TRIP12 and AREL1, which we show add K27-, K29- and K33- poly-ubiquitin chains on lysine residues in the ‘pro’ domain and destabilise pro-IL-1β. Mutation of ubiquitylation sites increased pro-IL-1β stability, but did not affect proteolysis by caspase-1. The extent of mature IL-1β production is therefore determined by precursor abundance, and UBE2L3, TRIP12 and AREL1 limit inflammation by shrinking the cellular pool of pro-IL-1β. Our study has uncovered fundamental processes governing IL-1β homeostasis and provided molecular insights that could be exploited to mitigate its adverse actions in disease.
The control of protein arginine phosphorylation facilitates proteostasis by an AAA+ chaperone protease system
Authors: Alver Regina , Hantke Ingo , Cornejo Fabián A. , Gunka Katrin , Rämisch Sebastian , Molière Noël , Charpentier Emmanuelle , Turgay Kürşad 
Published: September 2022

AbstractWe could demonstrate that the AAA+ unfoldase ClpC together with the protein arginine kinase and adaptor protein McsB, its activator McsA and the phosphatase YwlE form a unique chaperone system. Here, the McsA-activated McsB phosphorylates and targets aggregated substrate proteins for extraction and unfolding by ClpC. Sub-stoichiometric amounts of the YwlE phosphatase enhanced the ClpC/McsB/McsA mediated disaggregation and facilitated the de-phosphorylation of the arginine-phosphorylated substrate protein extruded by ClpC, allowing its subsequent refolding. Interestingly, the successfully refolded protein escaped degradation by the loosely associated ClpP protease. This unique chaperone system is thereby able to disaggregate and refold aggregated proteins but can also remove severely damaged protein aggregates by degradation.
Exploring the influence of telework on work performance in public services: experiences during the COVID-19 pandemic
Authors: Varotsis Nikolaos 
Published: September 2022

PurposeTelework has been widely used during the COVID-19 pandemic. However, distance work performed through teleworking may hinder organisational operations in public services owing to lower-than-expected work performance. This research paper aims to explore how teleworking relates to work performance and flexibility during the COVID-19 pandemic.Design/methodology/approachThe sample included 178 managers and employees in public services. The relationships between the variables were evaluated using linear regression.FindingsThe findings indicated that telework affected work performance in public services in different ways. This research also explored the relationship between work performance and work flexibility. The findings revealed that telework had not improved the efficiency of public service work performance; however, the implementation of flexible work schedules owing to teleworking has improved the work performance of public services.Research limitations/implicationsThis study only focussed on organisations operating in the public services in Greece.Practical implicationsTeleworking in public services may negatively affect organisational operations due to lower-than-expected work performance.Social implicationsThis study could assist managers by showcasing that telework may be better implemented to improve work performance through work flexibility rather than as organisational change.Originality/valueThis novel research aims to gain a better understanding of the impact of telework on factors such as work performance and flexibility.
Altruism and female entrepreneurship: evidence from the Turkish community in Kosovo
Authors: Skeja Afërina , Sadiku-Dushi Nora , Keskin Gülay 
Published: September 2022

Purpose
Altruism has an important place in shaping a healthy and developed society for the present and the future. Altruism refers to exhibiting good behavior that does not expect a reward in return. The fact that female entrepreneurs have busy working and private lives and try to be individuals who add value to life while striving for success in this intense tempo increases the investigation of altruism. This study aims to examine the differences in altruistic behaviors of female entrepreneurs according to demographic variables.

Design/methodology/approach
The research sample consists of 120 female entrepreneurs belonging to the Turkish community in Kosovo. T-test and one-way ANOVA analysis were applied to examine the difference between altruism and demographic characteristics.

Findings
This study determined that age and work experience influence altruism. Female entrepreneurs in the age group of 31+ had higher altruism scores than female entrepreneurs in the 21–24 and 25–30 age group. Additionally, it has been revealed that female entrepreneurs with 10 or more years of work experience have higher altruism scores than female entrepreneurs with under 1 year and 1–3 years of work experience. Findings also demonstrated that marital status, education level and income do not influence female entrepreneurs’ altruistic behaviors.

Research limitations/implications
Results obtained from the research are limited to the responses given by the participants to the survey used within the scope of the research.

Originality/value
As Turkish women are small community living in the country, it was interesting to see that apart from their entrepreneurial spirit do they also have altruistic behavior and how this behavior differs among different demographic characteristics. To the best of the authors’ knowledge, this research will contribute to the literature as no similar study was done before.
Assessing the relationship between service quality, satisfaction and loyalty: the Vietnamese higher education experience
Authors: Bui Ha Thi Thu , Bui Quyen Thi Tu , Nguyen Thanh Thi Phuong , Cao Quang Huu , Phung Thuy Van , Nguyen Ha Thanh 
Published: September 2022

Purpose
Service quality has been widely recognized as the core value of any higher education institution (HEIs), especially in the context of higher education reform in Vietnam. The paper aims to assess the student’s perceived service quality using SERVPERF scale and to find the relations between perceived service quality, satisfaction and loyalty in one public university in Vietnam.

Design/methodology/approach
The self-structured questionnaire using SERVPERF scale was administered to 350 students currently attending full-time courses at the university. Confirmatory factor analysis was used to certify the elementary factors. The regression models were applied to determine the relationship between service quality, student satisfaction and student loyalty.

Findings
The findings revealed the significant relationships between student perceived service quality with satisfaction and loyalty. However, this relationship is mediated by student satisfaction.

Research limitations/implications
The study is limited to public university education service and examined the perspective of student as “primary customer”; therefore, generalizing the results to other service industries and other role of student as stakeholder or citizen should be used with caution.

Practical implications
The SERVPERF scale could be delivered regularly to get the student’s perception on the HEIs performance. The results should be taken for purpose of continuing quality improvement to enhance the satisfaction and loyalty, particularly in the context of highly competitive environment and university autonomous in Vietnam.

Originality/value
This is believed to be the first study in Vietnam with sufficient evidence to support the concept that service quality positively related to students’ satisfaction and loyalty and satisfaction as mediating factor.
The pan‐genome of Splendidus clade species in the familyVibrionaceae: Insights into evolution, adaptation, and pathogenicity
Authors: Jiang Chunqi , Kasai Hisae , Mino Sayaka , Romalde Jesús L. , Sawabe Tomoo 
Published: September 2022

AbstractThe Splendidus clade is the largest clade inVibrionaceae, and its members are often related to mortality of marine animals with huge economic losses. The molecular bases of their pathogenicity and virulence, however, remain largely unknown. In particular, the complete genome sequences of the Splendidus clade species are rarely registered, which is one of the obstacles to predict core and/or unique genes responsible for their adaptation and pathogenicity, and to perform a fine scale meta‐transcriptome during bacterial infection to their hosts. In this study, we obtained the complete genomes of all type strains in the Splendidus clade and revealed that (1) different genome sizes (4.4–5.9 Mb) withV. lentusthe biggest and most of them had several big plasmids, likely because of the different features on mobilome elements; (2) the Splendidus clade consists of 19 species exceptV. cortegadensis, and 3 sub‐clades (SC) were defined with the 15 most closely related members as SC1; (3) different carbohydrate degradation preferences may be the result of environmental adaptation; and (4) a broad prediction of virulence factors (VFs) revealed core and species unique VF genes.
Efficacy and safety of second‐line chemotherapy for patients with advanced non‐small cell lung cancer complicated by interstitial lung disease
Authors: Otsuka Kenji , Nokihara Hiroshi , Mitsuhashi Atsushi , Ozaki Ryohiko , Yabuki Yohei , Yoneda Hiroto , Ogino Hirokazu , Nishioka Yasuhiko 
Published: September 2022

AbstractBackgroundTreatment of non‐small cell lung cancer (NSCLC) patients with interstitial lung disease (ILD) is limited because of the risk of its acute exacerbation (AE). Furthermore, the efficacy and safety of second‐line chemotherapy for these patients is unclear.MethodsTo investigate the efficacy and safety of second‐line chemotherapy for NSCLC patients with ILD, we retrospectively reviewed patients who were treated at our institute between April 2010 and December 2018.ResultsThirty‐five patients received two or more regimens. Thirty‐four patients were male and the median age at the initiation of second‐line chemotherapy was 70 years. Almost all patients had a smoking history. Fourteen patients had adenocarcinoma and 15 had squamous cell carcinoma histology. Stages III and IV were observed in 20 and 11 patients, respectively. With respect to the type of ILD, 12 patients had usual interstitial pneumonia (UIP). The overall response rate and disease control rate were 11.4 and 68.6%, respectively. The median progression‐free and median overall survival were 4.1 and 6.4 months, respectively. The AE of ILD was observed in eight patients, five of whom died. UIP and low percentage vital capacity were detected as significant risk factors for the AE of ILD.ConclusionSecond‐line chemotherapy among patients with NSCLC complicated by ILD showed a certain effectiveness, but some patients experienced the AE of ILD, which may lead to death. The risk of the AE of ILD must be considered especially for patients with UIP and low percentage VC.
The maternal prenatal and offspring early‐life gut microbiome of childhood asthma phenotypes
Authors: Lee‐Sarwar Kathleen A. , Chen Yih‐Chieh , Chen Yuan Yao , Kozyrskyj Anita L. , Mandhane Piush J. , Turvey Stuart E. , Subbarao Padmaja , Bisgaard Hans , Stokholm Jakob , Chawes Bo , Sørensen Søren J. , Kelly Rachel S. , Lasky‐Su Jessica , Zeiger Robert S. , O'Connor George T. , Sandel Megan T. , Bacharier Leonard B. , Beigelman Avraham , Carey Vincent J. , Harshfield Benjamin J. , Laranjo Nancy , Gold Diane R. , Weiss Scott T. , Litonjua Augusto A. 
Published: September 2022

AbstractBackgroundThe infant fecal microbiome is known to impact subsequent asthma risk, but the environmental exposures impacting this association, the role of the maternal microbiome, and how the microbiome impacts different childhood asthma phenotypes are unknown.MethodsOur objective was to identify associations between features of the prenatal and early‐life fecal microbiomes and child asthma phenotypes. We analyzed fecal 16 s rRNA microbiome profiling and fecal metabolomic profiling from stool samples collected from mothers during the third trimester of pregnancy (n = 120) and offspring at ages 3–6 months (n = 265), 1 (n = 436) and 3 years (n = 506) in a total of 657 mother–child pairs participating in the Vitamin D Antenatal Asthma Reduction Trial. We used clinical data from birth to age 6 years to characterize subjects with asthma as having early, transient or active asthma phenotypes. In addition to identifying specific genera that were robustly associated with asthma phenotypes in multiple covariate‐adjusted models, we clustered subjects by their longitudinal microbiome composition and sought associations between fecal metabolites and relevant microbiome and clinical features.ResultsSeven maternal and two infant fecal microbial taxa were robustly associated with at least one asthma phenotype, and a longitudinal gut microenvironment profile was associated with early asthma (Fisher exact test p = .03). Though mode of delivery was not directly associated with asthma, we found substantial evidence for a pathway whereby cesarean section reduces fecal Bacteroides and microbial sphingolipids, increasing susceptibility to early asthma.ConclusionOverall, our results suggest that the early‐life, including prenatal, fecal microbiome modifies risk of asthma, especially asthma with onset by age 3 years.
Bypassing the post‐anaesthesia care unit after elective hip and knee arthroplasty: a prospective cohort safety study
Authors: Nielsen N. I. , Kehlet H. , Gromov K. , Troelsen A. , Foss N. B. , Aasvang E. K. 
Published: September 2022

SummaryFollowing knee and hip arthroplasty, transfer to a recovery area immediately following surgery and before going to ward might be unnecessary in low‐risk patients. Avoiding the recovery area in this way could allow for more targeted use of resources for higher risk patients, which may improve operating theatre flow and productivity. A prospective single‐centre cohort study on the safety of criteria for bypassing the post‐anaesthesia care unit in elective hip and knee arthroplasty was designed. Criteria were: ASA physical status < 3; peri‐operative bleeding < 500 ml; low postoperative discharge‐score (modified Aldrete‐score); and an uncomplicated surgical and neuraxial anaesthesia procedure. The primary outcome was the number of patients in need of secondary readmission to the post‐anaesthesia care unit. Events within the first 24 postoperative hours were recorded, along with readmission and complication rates. A total of 696 patients were included, with 287 (41%) undergoing total hip arthroplasty, 274 (39%) undergoing total knee arthroplasty and 135 (19%) undergoing unicompartmental knee‐arthroplasty. Of these, 307 (44%) bypassed the post‐anaesthesia care unit. Patients all received multimodal analgesia without peripheral nerve blockade. Only one patient in the ward group required secondary readmission to the post‐anaesthesia care unit. Within 24 h, 151 events were reported, with 41 (27%) in the ward group and 110 (73%) in the post‐anaesthesia care unit group. Two events in each group occurred within 2 hours of surgery. No complications were attributed to bypassing the post‐anaesthesia care unit. The use of simple pragmatic criteria for bypassing the post‐anaesthesia care unit for patients undergoing knee and hip arthroplasty with spinal anaesthesia is possible and associated with significant reduction of post‐anaesthesia care unit admission and without apparent safety issues. Confirmation is needed from other studies and external validity should be interpreted cautiously in centres with different peri‐operative regimens, organisational and staffing structures.
The salvage of mesh infection after hernia repair with the use of negative pressure wound therapy (NPWT), a systematic review
Authors: Li Junsheng , Wang Yong , Shao Xiangyu , Cheng Tao 
Published: September 2022

AbstractBackgroundMesh infection is the most feared postoperative complication after abdominal wall hernia repair, often needs mesh removal. Negative pressure wound therapy (NPWT) has been used in these situations with diverse results. The aim of this study was to investigate the efficacy of the NPWT in the treatment of mesh infection, the primary outcome was the mesh salvage rates of different type of meshes and mesh positions.MethodsMajor databases were searched using the keywords negative pressure wound therapy, VSD, vacuum assisted, hernia, mesh infection, including various combinations of the terms. All relevant articles and reference lists in these original studies were also obtained from the above databases.ResultsTen articles containing 265 patients on the treatment of mesh infection after hernia repair with the use of NPWT method were included. The general infected mesh salvage rate with NPWT was 76.2%. The highest mesh salvage rate was achieved in polypropylene mesh (93.5%), followed by Proceed mesh (83.3%), and the mesh salvage rate was lower in polyester mesh (PCO) (0%) and the ePTFE mesh (14/3%). The salvage rate was higher when mesh placed in the onlay position (82.6%) or retromuscular/sublay position (98.5%), but lower in the IPOM position (55.6%).ConclusionThe treatment of mesh infection after hernia repair should be individualized according to the mesh type, mesh position and the severity of infection. Infected mesh with favourable mesh materials (large pore and monofilament polypropylene) and favourable positions (onlay or sublay/retromuscular) can be salvaged with the use of NPWT based conservative method.
Association of concomitant headache with hypoperfusion in ischemic stroke: A multimodal CT‐based study
Authors: Ge Yi , Dong Wanli , Lu Haifeng , Zhang Ximeng , Huang Shicun , Yang Yi , Ni Jianqiang 
Published: September 2022

AbstractPrevious investigations indicate that vessel wall elasticity may contribute to the occurrence of an ischemic stroke‐associated headache. In this prospective study, the association between radiologic parameters of intracranial hemodynamic changes and concomitant headaches during the early phase of ischemic stroke was examined. Consecutive patients with acute ischemic stroke (AIS) from the First Affiliated Hospital of Soochow University were recruited and divided into two groups according to their questionnaire results and the International Classification of Headache Disorder 3 criteria. Baseline data, including stroke sub‐types and neurological function, at admission and discharge were collected. Non‐contrast computed tomography (CT), CT angiography, and CT perfusion were performed to assess intracranial hemodynamic changes. Multiple adjusted logistic models were used and possible confounding factors were included in sequential models. A total of 190 patients with AIS (93 headaches and 97 non‐headache) were recruited. There were significant differences between the two groups in gender, hypertension, Alberta stroke program early CT score, relative cerebral blood flow (rCBF), and relative cerebral blood volume (rCBV). Furthermore, rCBV (adjusted odds ratio [OR] 0.160; 95% confidence interval [CI], 0.055–0.461; p < 0.001) and rCBF (adjusted OR, 0.309; 95% CI, 0.113–0.844; p < 0.05) were significantly associated with concomitant headache during the early phase of AIS in fully adjusted models. After adjusting for sociodemographic characteristics and other confounding factors, p values for the ORs were robust and intensified. Patients with lower rCBV and rCBF tended to experience the concomitant headache during the early phase of AIS. Regional hypoperfusion and microcirculation might play an important role in this separate clinical entity.
Antigingivitis efficacy of a sodium bicarbonate toothpaste: Pooled analysis
Authors: Parkinson Charles R. , Butler Andrew , Ling Martin R. 
Published: September 2022

AbstractObjectiveThe objective of this study was to investigate the antigingivitis and antiplaque treatment effect of a toothpaste containing 67% w/w sodium bicarbonate, at the individual tooth site, tooth region and whole mouth (overall) level, by way of a pooled analysis of data from similarly designed clinical trials.MethodsSix randomized controlled GSKCH clinical trials, 12–24 weeks in duration, were selected based on pre‐specified criteria which included access to patient level data, pre‐treatment dental prophylaxis, >20 bleeding sites and mild–moderate gingivitis at screening and use of 67% sodium bicarbonate toothpaste and non‐sodium bicarbonate (regular) toothpaste (negative control) for ≥4 weeks. Efficacy outcomes comprised plaque index (TPI), modified gingival index (MGI) and gingival bleeding (bleeding index (BI), number of bleeding sites (BS)). Treatment comparisons were made using ANCOVA for whole mouth (overall) scores and by tooth site region (facial overall, lingual overall; margin/body overall, facial margin/body, lingual margin/body; papillae/interproximal overall, facial papillae/interproximal, lingual papillae/interproximal). Pooled data for BI, MGI, and TPI at individual tooth sites was plotted as a mouthmap to summarize treatment response (change from baseline) by tooth site, at the 24‐week timepoint.ResultsFor all measures, whole mouth and for all tooth regions at all post‐treatment timepoints, significant (p < 0.001) differences in favour of the 67% sodium bicarbonate toothpaste compared with control were observed. At the 24‐week timepoint, facial regions demonstrated greater improvements than lingual regions, with the greatest between treatment improvement seen for the facial‐papillae regions for bleeding (BS/BI), facial‐margin regions for MGI and facial‐body regions for TPI. All individual tooth sites demonstrated numerically greater reductions from baseline for the 67% sodium bicarbonate toothpaste than the control toothpaste following 24 weeks use, with the greatest improvements (change from baseline) seen for posterior and papillae tooth sites for bleeding, margin tooth sites for MGI and body tooth sites for TPI.ConclusionThis pooled analysis of patient level‐data, limited to GSKCH long‐term gingivitis clinical studies, demonstrates that twice daily use of a 67% sodium bicarbonate toothpaste effectively removes plaque from all tooth sites, and results in clinically significant improvements in measures of gingival health, overall and for all the tooth regions investigated, compared with a non‐sodium bicarbonate (regular) toothpaste following 24 weeks twice daily use.