bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Dynamics of Selenium in Dairy Agroecosystems
Authors: McKernan Claire , Meharg Caroline , Carey Manus , Meharg Andrew A. 
Published: October 2022

AbstractEnvironmental selenium deficiency and toxicity can result in Keshan disease and selenosis, respectively. Due to dietary preferences, milk is considered a primary source of selenium, where, in controlled environments milk selenium concentration reflects the selenium provided in fodder to lactating cows. However, the movement of selenium through agroecosystems is not well understood. Therefore, the aim of this current study was to investigate variables that are responsible for transfer of selenium from soil to milk. Investigated parameters include spatial variability, soil selenium status, season, herd diet and husbandry of cattle. Farm-based sample collections were carried out, where soil, grass, silage and milk were collected over forty-eight geographically spaced locations over Northern Ireland during both summer and winter. Selenium concentrations were determined using ICP-MS. Median selenium concentrations for soil (0.46 mg/kg DM), grass (0.06 mg/kg DM) silage (0.03 mg/kg DM) and milk (0.23 mg/kg DM). Results showed that soil selenium concentrations were significantly affected by the pH and organic matter composition of soil. Additionally, a statistically significant relationships between soil and grass selenium concentrations were also found. Despite these strong environmental relationships with grass, these relationships were not reflected in the milk selenium concentrations, indicating a disconnect between the selenium concentration in the agricultural environment and the milk from which it is derived. Selenium was higher in milk in winter months as compared to summer, related to supplemental feed practices over the winter when cows are housed. This study highlighted that cattle feed supplementation of feed with selenium, an essential element, was an effective way of enhancing dietary exposure through a commonly consumed foodstuffs derived from dairy.
Stacked Lateral Gate-All-Around Metal–Oxide–Semiconductor Field-Effect Transistors and Their Three-Dimensional Integrated Circuits
Authors: Ye Shujun , Liu Liwei , Ma Yuanxiao , Wang Yeliang 
Published: October 2022

AbstractAccording to the International Roadmap for Devices and Systems, gate-all-around (GAA, also known as a surrounding gate) metal–oxide–semiconductor field-effect transistor (MOSFET) will be the main device in integrated circuits (ICs). Lateral GAA (LGAA) MOSFETs have been applied in CMOS logic circuits from a 3-nm technology node. However, further shrinkage of the contacted gate pitch is difficult owing to the physics and processing limitations. Three-dimensional (3D) stacking of chips or wafers is therefore widely studied for high integration. However, the device distance between stacked chips or wafers is rarely less than 10 µm, which is too long considering the electrical resistance and transfer delay, especially for logic circuits. Complementary field-effect transistors are currently a widely used 3D logic device; however, a compatible process is required for the heterostructures. The authors previously developed a fabrication process for symmetric-source/drain vertical GAA (referred to as ultimate VGAA, UVGAA) MOSFET for the first time; a novel architectural 3D IC with stacking UVGAA-based devices (CMOS and/or SRAM) in the vertical direction was also developed. In this perspective, a fabrication process for stacked LGAA (SLGAA) MOSFETs in the vertical direction is proposed for the first time and a high integration 3D logic IC based on SLGAA MOSFETs is also developed. These novel 3D architectures lay the foundations for next-generation ICs.
‘…and yet there’s still no peace’ Catholic Indigenous Residential Schools in Canada
Authors: McKinney Stephen J. 
Published: October 2022

AbstractPope Francis met representatives of the Indigenous peoples of Canada in Rome in April 2022 and in Canada in July 2022. At these meetings he offered sincere apologies for the ways in which the Catholic Church had colluded with the strategy of cultural assimilation of the Indigenous people in Canada. This was especially abhorrent in the residential schools, operated by the Catholic Church (and other Christian churches), that aimed to ‘evangelise’ and ‘civilise’ the Indigenous children. He emphasised that this was a ‘disastrous error’ and the ‘overall effects of the policies linked to the residential schools were catastrophic’. He was very clear at both meetings that this abuse was ‘incompatible with the Gospel of Jesus Christ’. These Catholic residential schools and the impact of these schools on the Indigenous people are discussed in this article.
Economic Enfranchisement, Goal Setting, and Rural Development
Authors: Hackett Susan , Watson Philip 
Published: October 2022

AbstractThis analysis introduces a conceptual framework for economic enfranchisement and studies its effect on an individual’s likelihood to set strong financial goals. A conceptual and empirical model is developed to investigate how economic enfranchisement influences an individual’s likelihood to set a goal and the strength of that goal. The empirical analysis employs an ordered probit to account for the two-stage goal-setting and goal strength decision process. Results show that economic enfranchisement has a significant effect on an individual’s likelihood to set financial goals where more enfranchised individuals are more likely to set strong goals than their disenfranchised counterparts.
Analysis of anti-SARS-CoV-2 Omicron-neutralizing antibody titers in different vaccinated and unvaccinated convalescent plasma sources
Authors: Sullivan David J. , Franchini Massimo , Joyner Michael J. , Casadevall Arturo , Focosi Daniele 
Published: October 2022

AbstractThe latest SARS-CoV-2 variant of concern Omicron, with its immune escape from therapeutic anti-Spike monoclonal antibodies and WA-1 vaccine-elicited sera, demonstrates the continued relevance of COVID-19 convalescent plasma (CCP) therapies. Lessons learnt from previous usage of CCP suggests focusing on early outpatients and immunocompromised recipients, with high neutralizing antibody titer units. Here, we systematically review Omicron-neutralizing plasma activity data, and report that approximately 47% (424/902) of CCP samples from unvaccinated pre-Omicron donors neutralizes Omicron BA.1 with a very low geometric mean of geometric mean titers for 50% neutralization GM(GMT50) of ~13, representing a > 20-fold reduction from WA-1 neutralization. Non-convalescent subjects who had received two doses of mRNA vaccines had a GM(GMT50) for Omicron BA.1 neutralization of ~27. However, plasma from vaccinees recovering from either previous pre-Omicron variants of concern infection, Omicron BA.1 infection, or third-dose uninfected vaccinees was nearly 100% neutralizing against Omicron BA.1, BA.2 and BA.4/5 with GM(GMT(50)) all over 189, 10 times higher than pre-Omicron CCP. Fully vaccinated and post-BA.1 plasma (Vax-CCP) had a GM(GMT50) > 450 for BA.4/5 and >1,500 for BA.1 and BA.2. These findings have implications for both CCP stocks collected in prior pandemic periods and for future plans to restart CCP collections. Thus, Vax-CCP provides an effective tool to combat ongoing variants that escape therapeutic monoclonal antibodies.
Isotropic reconstruction for electron tomography with deep learning
Authors: Liu Yun-Tao , Zhang Heng , Wang Hui , Tao Chang-Lu , Bi Guo-Qiang , Zhou Z. Hong 
Published: October 2022

AbstractCryogenic electron tomography (cryoET) allows visualization of cellular structures in situ. However, anisotropic resolution arising from the intrinsic “missing-wedge” problem has presented major challenges in visualization and interpretation of tomograms. Here, we have developed IsoNet, a deep learning-based software package that iteratively reconstructs the missing-wedge information and increases signal-to-noise ratio, using the knowledge learned from raw tomograms. Without the need for sub-tomogram averaging, IsoNet generates tomograms with significantly reduced resolution anisotropy. Applications of IsoNet to three representative types of cryoET data demonstrate greatly improved structural interpretability: resolving lattice defects in immature HIV particles, establishing architecture of the paraflagellar rod in Eukaryotic flagella, and identifying heptagon-containing clathrin cages inside a neuronal synapse of cultured cells. Therefore, by overcoming two fundamental limitations of cryoET, IsoNet enables functional interpretation of cellular tomograms without sub-tomogram averaging. Its application to high-resolution cellular tomograms should also help identify differently oriented complexes of the same kind for sub-tomogram averaging.
Cellular glycan modification by B3GAT1 broadly restricts influenza virus infection
Authors: Trimarco Joseph D. , Nelson Sarah L. , Chaparian Ryan R. , Wells Alexandra I. , Murray Nathan B. , Azadi Parastoo , Coyne Carolyn B. , Heaton Nicholas S. 
Published: October 2022

AbstractCommunicable respiratory viral infections pose both epidemic and pandemic threats and broad-spectrum antiviral strategies could improve preparedness for these events. To discover host antiviral restriction factors that may act as suitable targets for the development of host-directed antiviral therapies, we here conduct a whole-genome CRISPR activation screen with influenza B virus (IBV). A top hit from our screen, beta-1,3-glucuronyltransferase 1 (B3GAT1), effectively blocks IBV infection. Subsequent studies reveal that B3GAT1 activity prevents cell surface sialic acid expression. Due to this mechanism of action, B3GAT1 expression broadly restricts infection with viruses that require sialic acid for entry, including Victoria and Yamagata lineage IBVs, H1N1/H3N2 influenza A viruses (IAVs), and the unrelated enterovirus D68. To understand the potential utility of B3GAT1 induction as an antiviral strategy in vivo, we specifically express B3GAT1 in the murine respiratory epithelium and find that overexpression is not only well-tolerated, but also protects female mice from a lethal viral challenge with multiple influenza viruses, including a pandemic-like H1N1 IAV. Thus, B3GAT1 may represent a host-directed broad-spectrum antiviral target with utility against clinically relevant respiratory viruses.
Glutamyl-prolyl-tRNA synthetase 1 coordinates early endosomal anti-inflammatory AKT signaling
Authors: Lee Eun-Young , Kim Su-Man , Hwang Jung Hwan , Jang Song Yee , Park Shinhye , Choi Sanghyeon , Lee Ga Seul , Hwang Jungwon , Moon Jeong Hee , Fox Paul L. , Kim Sunghoon , Lee Chul-Ho , Kim Myung Hee 
Published: October 2022

AbstractThe AKT signaling pathway plays critical roles in the resolution of inflammation. However, the underlying mechanisms of anti-inflammatory regulation and signal coordination remain unclear. Here, we report that anti-inflammatory AKT signaling is coordinated by glutamyl-prolyl-tRNA synthetase 1 (EPRS1). Upon inflammatory activation, AKT specifically phosphorylates Ser999 of EPRS1 in the cytoplasmic multi-tRNA synthetase complex, inducing release of EPRS1. EPRS1 compartmentalizes AKT to early endosomes via selective binding to the endosomal membrane lipid phosphatidylinositol 3-phosphate and assembles an AKT signaling complex specific for anti-inflammatory activity. These events promote AKT activation-mediated GSK3β phosphorylation, which increase anti-inflammatory cytokine production. EPRS1-deficient macrophages do not assemble the early endosomal complex and consequently exacerbate inflammation, decreasing the survival of EPRS1-deficient mice undergoing septic shock and ulcerative colitis. Collectively, our findings show that the housekeeping protein EPRS1 acts as a mediator of inflammatory homeostasis by coordinating compartment-specific AKT signaling.
Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients
Authors: Tran Hoang N. H. , Thu Trang Nguyen Hoang , Nguyen Phu Huu , Vo Chi Nguyen , Doan Khanh Van , Nguyen Ngoc Minh Chau , Nguyen Ngoc Tuan , Ta Van Ngoc Duc , Vu Khuong An , Hua Thanh Danh , Nguyen To Nguyen Thi , Van Tan Trinh , Pham Duc Trung , Duong Ba Lap , Nguyen Phuc Minh , Hoang Vinh Chuc , Pham Duy Thanh , Thwaites Guy E. , Hall Lindsay J. , Slade Daniel J. , Baker Stephen , Tran Vinh Hung , Chung The Hao 
Published: October 2022

AbstractPerturbations in the gut microbiome have been associated with colorectal cancer (CRC), with the colonic overabundance of Fusobacterium nucleatum shown as the most consistent marker. Despite its significance in the promotion of CRC, genomic studies of Fusobacterium is limited. We enrolled 43 Vietnamese CRC patients and 25 participants with non-cancerous colorectal polyps to study the colonic microbiomes and genomic diversity of Fusobacterium in this population, using a combination of 16S rRNA gene profiling, anaerobic microbiology, and whole genome analysis. Oral bacteria, including F. nucleatum and Leptotrichia, were significantly more abundant in the tumour microbiomes. We obtained 53 Fusobacterium genomes, representing 26 strains, from the saliva, tumour and non-tumour tissues of six CRC patients. Isolates from the gut belonged to diverse F. nucleatum subspecies (nucleatum, animalis, vincentii, polymorphum) and a potential new subspecies of Fusobacterium periodonticum. The Fusobacterium population within each individual was distinct and in some cases diverse, with minimal intra-clonal variation. Phylogenetic analyses showed that within four individuals, tumour-associated Fusobacterium were clonal to those isolated from non-tumour tissues. Genes encoding major virulence factors (Fap2 and RadD) showed evidence of horizontal gene transfer. Our work provides a framework to understand the genomic diversity of Fusobacterium within the CRC patients, which can be exploited for the development of CRC diagnostic and therapeutic options targeting this oncobacterium.
Scaffold-based bone tissue engineering in microgravity: potential, concerns and implications
Authors: Mochi Federico , Scatena Elisa , Rodriguez Daniel , Ginebra Maria-Pau , Del Gaudio Costantino 
Published: October 2022

AbstractOne of humanity’s greatest challenges is space exploration, which requires an in-depth analysis of the data continuously collected as a necessary input to fill technological gaps and move forward in several research sectors. Focusing on space crew healthcare, a critical issue to be addressed is tissue regeneration in extreme conditions. In general, it represents one of the hottest and most compelling goals of the scientific community and the development of suitable therapeutic strategies for the space environment is an urgent need for the safe planning of future long-term manned space missions. Osteopenia is a commonly diagnosed disease in astronauts due to the physiological adaptation to altered gravity conditions. In order to find specific solutions to bone damage in a reduced gravity environment, bone tissue engineering is gaining a growing interest. With the aim to critically investigate this topic, the here presented review reports and discusses bone tissue engineering scenarios in microgravity, from scaffolding to bioreactors. The literature analysis allowed to underline several key points, such as the need for (i) biomimetic composite scaffolds to better mimic the natural microarchitecture of bone tissue, (ii) uniform simulated microgravity levels for standardized experimental protocols to expose biological materials to the same testing conditions, and (iii) improved access to real microgravity for scientific research projects, supported by the so-called democratization of space.
Controllably grown single-crystal films as flexoelectric nanogenerators for continuous direct current output
Authors: Li Yang , Zhou Qinling , Wu Jiating , Xu Junhua , Shi Weilong , Su Chao , Chen Daifen , Shao Zongping 
Published: October 2022

AbstractUntil now, conventional nanogenerators could only produce electric pulses with relatively low-power densities. Herein, we invent a novel controllable growth technique for two-dimensional (2-D) cuprous oxide (p-Cu2O) single-crystal films, and on this basis, a new concept of 2-D single-crystal film flexoelectric nanogenerators (FENGs) are rationally designed and constructed for the first time, which has the characteristics of long-range order lattice, few grain boundaries and defects. More importantly, the accumulated built-in polarization potential in the bent 2-D p-Cu2O single-crystal film FENGs is in the same orientation as the output electricity, resulting in the first nanogenerator that can output continuous and stable electric signals with high voltage (Voc of 2.8 V), current (Jsc of 11.5 μA·cm−2) and power density (14.4 μW·cm−2), exhibiting great practical application potential for power generation and motion capture. This research breaks new ground and establishes a precedent for high-performance and continuous-output nanogenerators, as well as smart wearable sensors.
Multiorder hydrologic Position for Europe — a Set of Features for Machine Learning and Analysis in Hydrology
Authors: Nölscher Maximilian , Mutz Michael , Broda Stefan 
Published: October 2022

AbstractThe presented dataset EU-MOHP v013.1.1 provides multiscale information on the hydrologic position (MOHP) of a geographic point within its respective river network and catchment as gridded maps. More precisely, it comprises the three measures “divide to stream distance” (DSD) as sum of the distances to the nearest stream and catchment divide, “lateral position” (LP) as a relative measure of the position between the nearest stream and divide and “stream distance” (SD) as the distance to the nearest stream. These three measures are calculated for nine hydrologic orders to reflect different spatial scales from local to continental. Its spatial extent covers major parts of the European Economic Area (EEA39) which also largely coincides with physiographical Europe. Although there are multiple potential use cases, this dataset serves predominantly as valuable static environmental descriptor or predictor variable for hydrogeological and hydrological modelling such as mapping or forecasting tasks using machine learning. The generation of this dataset uses free open source software only and therefore can be transferred to other regions or input datasets.
A laser guide technique: a novel method for accurate acetabular cup alignment in total hip arthroplasty
Authors: Kohno Yusuke , Nakamura Tetsuro , Fujii Masanori , Shin Satoshi , Hara Toshihiko 
Published: October 2022

AbstractFor accurate cup alignment without navigation in total hip arthroplasty (THA), we developed a “laser guide technique.” The major purposes of this paper are to introduce the technique and compare its accuracy with a conventional manual technique. As a clinical outcome, the dislocation rate was reviewed. Our laser guide technique, which includes preoperative postural adjustment and intraoperative angular reference, has been detailed in the manuscript. 599 hips in 523 patients who underwent primary THA with piriformis-sparing posterolateral approach in April 2010–March 2016 were reviewed. Patients were divided into three groups: conventional group (135 hips), laser guide group (80 hips), and laser + radiographic alignment guide group (384 hips). Radiographic inclination (RI) and radiographic anteversion (RA) errors were evaluated. The dislocation rate was reviewed in 540 hips in 476 patients who were followed up > 2 years postoperatively. Absolute values of the RI/RA error in the three groups were 5.3° ± 4.0°/6.5° ± 4.5°, 4.0° ± 2.8°/4.9° ± 4.4°, and 3.3° ± 2.6°/3.6° ± 2.8°, respectively, indicating substantially enhanced accuracy with laser and radiographic alignment guide. The dislocation rates were 2.5% (3/119) and 0.2% (1/421) in the conventional and laser groups, respectively. Our novel laser guide technique considerably enhanced cup alignment accuracy, suggesting its potential applicability for THA in the lateral decubitus position.
Infection control for COVID-19 in hospital examination room
Authors: Takada Mamoru , Fukushima Taichi , Ozawa Sho , Matsubara Syuma , Suzuki Takeshi , Fukumoto Ichiro , Hanazawa Toyoyuki , Nagashima Takeshi , Uruma Reiko , Otsuka Masayuki , Tanaka Gaku 
Published: October 2022

AbstractHealthcare providers are vulnerable to infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) because of their close proximity to patients with coronavirus disease 2019. SARS-CoV-2 is mainly transmitted via direct and indirect contact with respiratory droplets, and its airborne transmission has also been identified. However, evidence for environmental factors is scarce, and evidence-based measures to minimize the risk of infection in clinical settings are insufficient. Using computational fluid dynamics, we simulated exhalation of large and small aerosol particles by patients in an otolaryngology examination room, where medical procedures require the removal of a face mask. The effects of coughing were analyzed, as well as those of humidity as a controllable environmental factor and of a suction device as an effective control method. Our results show that a suction device can minimize aerosol exposure of healthcare workers by efficiently removing both large (11.6–98.2%) and small (39.3–99.9%) aerosol particles. However, for coughing patients, the removal efficiency varies inversely with the particle size, and the humidity notably affects the aerosol behavior, indicating the need for countermeasures against smaller aerosols. Overall, these results highlight the potential and limitation of using a suction device to protect against SARS-CoV-2 and future respiratory infections.
High-gradient magnetic fields and starch metabolism: results from a space experiment
Authors: Hasenstein K. H. , Park M. R. , John S. P. , Ajala C. 
Published: October 2022

AbstractDirecting plant growth in weightlessness requires understanding the processes that establish plant orientation and how to manipulate them. Both gravi- and phototropism determine directional growth and previous experiments showed that high gradient magnetic fields (HGMF) can induce curvature in roots and shoots. Experiments with Brassica rapa verified that that gravitropism-like induction of curvature is possible in space and that the HGMF-responsive organelles are amyloplasts. We assessed the effect of space and HGMF based on 16 genes and compared their transcription with static growth and clinorotation. Amyloplasts size in root tips increased under weightlessness but decreased under clinorotation but not in response to magnetic fields. Amyloplast size changes were correlated with reduced amylase transcription in space samples and enhanced transcription after clinorotation. Mechanostimulation and weightlessness have opposite effects on the size of amyloplasts. The data show that plants perceive weightlessness, and that their metabolism adjusts to microgravity and mechanostimulation. Thus, clinorotation as surrogate for space research may lead to incorrect interpretations.