bluuebunny/crossref_metadata_2025_split huggingface dataset:
PP2 protects from keratin mutation–associated liver injury and filament disruption via SRC kinase inhibition in male but not female mice
Authors: Li Pei , Maitra Dhiman , Kuo Ning , Kwan Raymond , Song Yang , Tang Weiliang , Chen Lu , Xie Qing , Liu Li , Omary M. Bishr 
Published: May 2022

Background and Aims:
Hepatocyte keratin polypeptides 8/18 (K8/K18) are unique among intermediate filaments proteins (IFs) in that their mutation predisposes to, rather than causes, human disease. Mice that overexpress human K18 R90C manifest disrupted hepatocyte keratin filaments with hyperphosphorylated keratins and predisposition to Fas‐induced liver injury. We hypothesized that high‐throughput screening will identify compounds that protect the liver from mutation‐triggered predisposition to injury.

Approach and Results:
Using A549 cells transduced with a lentivirus K18 construct and high‐throughput screening, we identified the SRC‐family tyrosine kinases inhibitor, PP2, as a compound that reverses keratin filament disruption and protects from apoptotic cell death caused by K18 R90C mutation at this highly conserved arginine. PP2 also ameliorated Fas‐induced apoptosis and liver injury in male but not female K18 R90C mice. The PP2 male selectivity is due to its lower turnover in male versus female livers. Knockdown of SRC but not another kinase target of PP2, protein tyrosine kinase 6, in A549 cells abrogated the hepatoprotective effect of PP2. Phosphoproteomic analysis and validation showed that the protective effect of PP2 associates with Ser/Thr but not Tyr keratin hypophosphorylation, and differs from the sex‐independent effect of the Ser/Thr kinase inhibitor PKC412. Inhibition of RAF kinase, a downstream target of SRC, by vemurafenib had a similar protective effect to PP2 in A549 cells and male K18 R90C mice.

Conclusions:
PP2 protects, in a male‐selective manner, keratin mutation‐induced mouse liver injury by inhibiting SRC‐triggered downstream Ser/Thr phosphorylation of K8/K18, which is phenocopied by RAF kinase inhibitor vemurafenib. The PP2/vemurafenib‐associated findings, and their unique mechanisms of action, further support the potential role of select kinase inhibition as therapeutic opportunities for keratin and other IF‐associated human diseases.
Mammographic mass recognition using feature reuse and channel attention mechanism
Authors: Pan Ansi , Xu Shengzhou 
Published: May 2022

AbstractMass recognition is one of the important steps in early diagnosis for breast cancer. However, one of the major issues in mass recognition is low sensitivity. In this study, a novel convolutional neural network using feature reuse and channel attention mechanism is proposed and evaluated for mass recognition in mammograms. To improve representational power of network, our model takes advantage of two advanced techniques: one is feature reuse for exploiting the potential of the network through directing connections, and the other is the channel attention mechanism for modelling the interdependencies between channels by integrating the channel‐wise information. The performance of our model is evaluated on DDSM and the values of the evaluation are 92.05%, 91.25%, 91.25%, 92.71%, 0.91 and 0.96 in terms of accuracy, precision, sensitivity, specificity, F1_score and AUC, respectively. The experimental results demonstrate the effectiveness of the proposed model to mass recognition task in mammograms.
LINC00941 promoted in vitro progression and glycolysis of laryngocarcinoma by upregulating PKM via activating the PI3K/AKT/mTOR signaling pathway
Authors: Li Zhihai , Jin Qiaozhi , Sun Yana 
Published: May 2022

AbstractBackgroundLINC00941 has been proved to be related to various tumors, but its relationship with laryngocarcinoma remains vague.MethodsLINC00941 expression in laryngocarcinoma tumor and laryngocarcinoma cells was determined by real time‐quantitative polymerase chain reaction (RT‐qPCR). Besides, the five‐year survival of laryngocarcinoma patients with different LINC00941 expression was analyzed with Kaplan–Meier survival analysis, and the clinical characteristics of laryngocarcinoma patients were also recorded. After transfection, cell viability, cell proliferation, apoptosis, cell cycle, migration, and invasion were detected by cell counting kit‐8 (CCK‐8), colony formation, flow cytometry, cell scratch, and Transwell assays, respectively. Glycolysis was assessed by the colorimetric method. Expressions of proliferation‐associated proteins, migration‐associated proteins, glycolysis‐associated proteins, and phosphatidylinositol 3‐kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signal pathway‐associated proteins were detected by Western blot.ResultsIn laryngocarcinoma tumor tissues and cells, LINC00941 was highly expressed. High expression of LINC00941 decreased the 5‐year survival of laryngocarcinoma patients, and it was positively related to lymph node metastasis and clinical stages. LINC00941 overexpression decreased apoptosis but promoted cell viability, proliferation, cell‐cycle progression, migration, and invasion, and glucose consumption and lactate production in laryngocarcinoma cells. Moreover, LINC00941 overexpression elevated expressions of Ki‐67, PCNA, MMP2, N‐Cadherin, HK2, PFKFB4, and PKM, activated the PI3K/AKT/mTOR signal pathway but reduced E‐Cadherin expression, while LINC00941 silencing had the opposite effects. PKM overexpression reversed the effects of LINC00941 silencing on cellular and glycolytic phenotypes.ConclusionLINC00941 promoted in vitro progression and glycolysis of laryngocarcinoma cells by upregulating PKM via activating the PI3K/AKT/mTOR signaling pathway.
SARS‐CoV‐2 Omicron BA.1 variant breakthrough infections in nursing home residents after an homologous third dose of the Comirnaty® COVID‐19 vaccine: Looking for correlates of protection
Authors: Torres Ignacio , Giménez Estela , Albert Eliseo , Zulaica Joao , Álvarez‐Rodríguez Beatriz , Burgos Javier S. , Peiró Salvador , Limón Ramón , Vanaclocha Hermelinda , Rodado Celia , Botija Pilar , Sifre Amelia , Tur Borja , Lozano Rosa Andrés , Orosa Iria , Vicente‐Ruiz MªÁngeles , Carrión Ramón J. , Clari Maria Á. , Sánchez‐Payá José , Díez‐Domingo Javier , Comas Iñaki , González‐Candelas Fernando , Geller Ron , Navarro David ,   the Valencian vaccine research program (ProVaVac) study group
Published: May 2022

AbstractWe investigated whether peripheral blood levels of SARS‐CoV‐2 Spike (S) receptor binding domain antibodies (anti‐RBD), neutralizing antibodies (NtAb) targeting Omicron S, and S‐reactive‐interferon (IFN)‐γ‐producing CD4+and CD8+T cells measured after a homologous booster dose (3D) with the Comirnaty® vaccine was associated with the likelihood of subsequent breakthrough infections due to the Omicron variant. An observational study including 146 nursing home residents (median age, 80 years; range, 66–99; 109 female) evaluated for an immunological response after 3D (at a median of 16 days). Anti‐RBD total antibodies were measured by chemiluminescent immunoassay. NtAb were quantified by an Omicron S pseudotyped virus neutralization assay. SARS‐CoV‐2‐S specific‐IFNγ‐producing CD4+and CD8+T cells were enumerated by whole‐blood flow cytometry for intracellular cytokine staining. In total, 33/146 participants contracted breakthrough Omicron infection (symptomatic in 30/33) within 4 months after 3D. Anti‐RBD antibody levels were comparable in infected and uninfected participants (21 123 vs. 24 723 BAU/ml;p = 0.34). Likewise, NtAb titers (reciprocal IC50titer, 157 vs. 95;p = 0.32) and frequency of virus‐reactive CD4+(p = 0.82) and CD8+(p = 0.91) T cells were similar across participants in both groups. anti‐RBD antibody levels and NtAb titers estimated at around the time of infection were also comparable (3445 vs. 4345 BAU/ml;p = 0.59 and 188.5 vs. 88.9;p = 0.70, respectively). Having detectable NtAb against Omicron or SARS‐CoV‐2‐S‐reactive‐IFNγ‐producing CD4+or CD8+T cells after 3D was not correlated with increased protection from breakthrough infection (OR, 1.50;p = 0.54; OR, 0.0;p = 0.99 and OR 3.70;p = 0.23, respectively). None of the immune parameters evaluated herein, including NtAb titers against the Omicron variant, may reliably predict at the individual level the risk of contracting COVID‐19 due to the Omicron variant in nursing home residents.
Frequency and distribution of H1N1 influenza A viruses with oseltamivir‐resistant mutations worldwide before and after the 2009 pandemic
Authors: Zhang Weixu , Xu Hefeng , Guan Shuxuan , Wang Chengmin , Dong Guoying 
Published: May 2022

AbstractH1N1 influenza has brought serious threats to people's health and a high socioeconomic burden to society. Oseltamivir, a kind of neuraminidase (NA) inhibitor, is the second‐generation specific drug that is broadly used currently. However, H1N1 influenza viruses have exhibited oseltamivir resistance in the past decades, which might be a hidden danger. To understand the frequency and distribution laws of oseltamivir‐resistant viruses, we conducted a thorough and deep analysis of the available NA protein sequences of H1N1 influenza viruses worldwide from 1918 to 2020. The differences and similarities before and after 2009 were also considered since the dominant viruses changed in this period. Results showed that 3.76% of H1N1 viruses harbored oseltamivir resistance currently. Among various significative mutations, H274Y had the highest frequency of 3.30%, while the frequencies of the other mutations were far below this whether before or after 2009. The oseltamivir resistance was mainly found in three hosts, humans, swine, and avian. Different mutation sites could exhibit different distributions in each host. Our results showed that the resistance level reached a peak during the 2007–2008 influenza season and then quickly decreased in 2009. The resistance also displayed a global distribution. The densely populated countries usually had a high resistance level. However, frequent significative mutations were also found in some small countries. Our findings indicated the necessity of monitoring oseltamivir resistance around the world. The study could provide a unique perspective toward the cognition of viruses and facilitate the future study of both pandemic and drug development.
Immunogenicity and safety of an inactivated SARS‐CoV‐2 vaccine in people living with HIV: A cross‐sectional study
Authors: Cai Shaohang , Liao Guichan , Yu Tao , Gao Qiqing , Zou Lirong , Zhang Huan , Xu Xuwen , Chen Juanjuan , Lu Aili , Wu Yingsong , Li Baisheng , Peng Jie 
Published: May 2022

AbstractWe aimed to analyze the efficacy and safety of an inactivated SARS‐CoV‐2 vaccine in people living with HIV (PLWH). A total of 143 PLWH and 50 healthy individuals were included in this study. A commercially available magnetic chemiluminescence enzyme immunoassay kit was used to detect serum IgG and IgM antibodies against SARS‐CoV‐2. Serum levels of SARS‐CoV‐2‐specific IgG were significantly higher in the control group than in the PLWH group (p = 0.001). Overall, 76% of individuals in the control group were detected with seropositivity IgG against SARS‐CoV‐2 compared to 58% in the PLWH group (p = 0.024). In PLWH with IgG seropositivity, CD4+ T‐cell counts before antiretroviral therapy (ART) was higher (p = 0.015). Multivariable analysis indicated that CD4+ T cells at IgG detection (odds ratio [OR] = 1.004, p = 0.006) and time after vaccination (OR = 0.977, p = 0.014) were independently associated with seropositivity IgG against SARS‐CoV‐2 in PLWH. Neutralizing antibody (nAb) titers in PLWH against wild‐type SARS‐CoV‐2 were similar to those in the control group (p = 0.160). The proportion of seropositive nAbs against wild‐type SARS‐CoV‐2 was also similar (95% in the control group vs. 97% in the PLWH group, p = 0.665). Similar results were obtained when nAb was detected against the delta variants with similar titers (p = 0.355) and a similar proportion of seropositive nAbs were observed (p = 0.588). All the side effects observed in our study were mild and self‐limiting. The inactivated COVID‐19 vaccine appears to be safe with good immunogenicity in Chinese PLWH.
Risk Factors For Hearing Decline From Childhood To Early Adolescence
Authors: Paping Danique E. , Oosterloo Berthe C. , El Marroun Hanan , Homans Nienke C. , Baatenburg de Jong Rob J. , van der Schroeff Marc P. , Vroegop Jantien L. 
Published: May 2022

ObjectiveTo identify risk factors of hearing decline between 9 and 13 years of age. The risk factors examined included sociodemographic, health, and lifestyle‐related factors.MethodsThis study was embedded within a population‐based prospective cohort study from fetal life onwards in the Netherlands. Pure‐tone audiometry and tympanometry were performed at the age of 9 and 13 years. The hearing decline was defined as an increase in low‐frequency or high‐frequency pure‐tone average of at least 5 dB in one of both ears. Multivariable logistic regression was performed to examine the association of possible risk factors with hearing decline. The study was conducted from April 2012 to October 2015, and from April 2016 to September 2019.ResultsOf the 3,508 participants included, 7.8% demonstrated a hearing decline in the low frequencies, and 11.3% in the high frequencies. Participants who reported alcohol consumption were more likely to have a hearing decline in the low frequencies (OR 1.5, 95% CI 1.1; 2.0). Moreover, a lower educational level was associated with an increased odds of having a hearing decline in the high frequencies (OR 1.4, 95% CI 1.0; 1.8). Age, sex, household income, personal music player use, and body mass index were not associated with hearing decline.ConclusionEducational level and risky behavior were significantly associated with hearing decline from childhood to early adolescence. The findings of the present study can help in the design of public health interventions to prevent hearing loss at a young age.Level of Evidence2 (prospective cohort study) Laryngoscope, 133:389–395, 2023
Invisible NIR Spectral Imaging and Laser‐Induced Thermal Imaging of Na(Nd/Y)F4@glass with Opposite Effect for Optical Security
Authors: Wang Shaoxiong , Zhu Jiwen , He Youwu , Li Zhifang , Lin Jidong , Liao Shengxiang , Huang Feng , Huang Ping , Zheng Yuanhui , Li Xiaoyan , Chen Daqin 
Published: May 2022

AbstractCurrently, advanced optical security techniques are imminent in response to the increasingly rampant counterfeit and pirated goods on a global scale. Herein, hexagonal Na(NdxY1−x)F4 (x = 0–1) solid‐solution nanocrystals (NCs) embedded glasses are successfully fabricated via in situ glass crystallization. Benefited from the protection of inorganic glass, Na(NdxY1−x)F4 NCs exhibit superior long‐term stability and can endure high‐power 808 nm laser irradiation. Importantly, opposite variations between near‐infrared (NIR) luminescence and laser‐induced temperature can be detected for the nanocomposites, which are sensitive to Nd3+ doping concentration. As a proof‐of‐concept experiment, Nd3+ concentration dependent anti‐counterfeiting patterns with high‐concealment and high‐precision are designed and show bright emission and dimmish thermal images in Nd3+‐tenuous composites under the irradiation of 808 nm laser, while opposite displays can be achieved in Nd3+‐enriched one. This kind of information encryption triggered by a commercial single light source to achieve dual opposing displays in one material can effectively improve security in anti‐counterfeiting.
NIR Responsive Injectable Nanocomposite Thermogel System Against Osteosarcoma Recurrence
Authors: Guo Ranran , Zhang Peng , Liu Jianing , Xie Ruihong , Wang Leyu , Cai Liu , Qiu Xiaozhong , Sang Hongxun 
Published: May 2022

AbstractCompared to traditional postoperative radiation and chemotherapy, immune checkpoint blockade (ICB) therapy demonstrates superiority by provoking own immune system to cure cancer completely even for some terminally ill patients. However, systemic administration of ICB is liable to cause severe immunity inflammation or immune storm. Here, an injectable, near infrared (NIR) responsive, multifunctional nanocomposite thermogel as a local ICB delivery system for cancer postsurgical therapy is proposed. By copolymerization of thermosensitive and zwitterionic monomer, the injectable thermogel with adjustable sol–gel transition temperature is obtained. Afterward, combined with functional mesoporous nanoparticles, the platform can absorb NIR light and transfer it into heat. The generated heat will promote retro Diels–Alder (D‐A) reaction to degrade coating layer on nanoparticle, achieving NIR controlled ICB release. Furthermore, the local ICB delivery system is applied on an osteosarcoma postsurgical recurrence model and results indicate the platform with favorable biocompatibility can avoid early leakage of cargos and greatly increase drug content at tumor site. Besides, long‐term controlled ICB release of the system effectively improves the amount of active T cells, resulting in excellent antitumor recurrence effect. Overall, this work suggests the local injectable nanocomposite thermogel is expected to be a promising tool for cancer postoperative therapy.
Risk of delirium associated with antimuscarinics in older adults: A case‐time‐control study
Authors: Nishtala Prasad S. , Chyou Te‐Yuan 
Published: May 2022

AbstractBackgroundOlder adults are at an increased risk of delirium because of age, polypharmacy, multiple comorbidities and acute illness. Antimuscarinics are the backbone of the pharmacological management of overactive bladder. However, the safety profiles of antimuscarinics vary because of their dissimilarities to muscarinic receptor‐subtype affinities and are associated with differential central anticholinergic adverse effects.ObjectiveThis study aimed to examine delirium risk in new users of oxybutynin and solifenacin in older adults (≥ 65 years). In the secondary analyses, we examined the risk of delirium by type and dose of antimuscarinic.MethodWe applied a case‐time‐control design to investigate delirium risk in older adults who started taking oxybutynin and solifenacin. We used a nationwide inpatient hospital data (2005–2016), National Minimum Data Set, maintained by the Ministry of Health, New Zealand (NZ), to identify older adults with a new‐onset diagnosis of delirium. Eligible patients were older adults aged 65 at entry into the cohort on 1/1/2006. We used dispensing claims data to determine antimuscarinic treatment exposure. The antimuscarinic included in the study were new users of oxybutynin and solifenacin. These two antimuscarinics are subsidised by the Pharmaceutical Management Agency and are the most frequently used antimuscarinic in NZ. A conditional logistic regression model was used to compute matched odds ratios (MORs) and 95% confidence intervals (CIs). In the case‐time‐control design, we made separate analyses to evaluate the dose–response risk of delirium.ResultsWe identified 4818 individuals (mean age 82.14) from 2005 to 2015 with incident delirium and were exposed to at least one of the antimuscarinic of interest. The case‐time‐control matched odds ratio (MOR) for delirium with oxybutynin was (2.06, 95% confidence interval [CI] 1.07–3.96). Solifenacin was not associated with delirium (0.89 95%CI 0.64–1.23). In the sensitivity analyses, the case‐time‐control MOR for delirium using a shorter risk period (0–3 days) did not change the results. The dose–response risk of delirium was significant for oxybutynin (0.05, 95%CI 0.02–0.08) but not for solifenacin (−0.01, 95%CI −0.03 to 0.00). In addition, in the subgroup analyses, a statistically significant association of delirium was found for oxybutynin but not for solifenacin in the non‐dementia cohort (2.11,95% CI 1.08–4.13) and the dementia cohort (1.25, 95%CI 0.05–26.9).ConclusionThe study found that oxybutynin but not solifenacin is associated with a risk of new‐onset delirium in older adults. The higher blockade of M1 and M2 receptors by oxybutynin is likely to contribute to delirium than solifenacin, which is highly selective for the M3 receptor subtype. Therefore, the treatment choice with an M3 selective agent must be given due consideration, particularly in those with pre‐existing cognitive impairment.
In situ constructed Ti3C2Tx MXene/polypyrrole composite with enhanced sodium storage capacity for efficient hybrid capacitive deionization
Authors: Tang Jian , Chen Zeqiu , Chen Yaoyu , Xu Xingtao , Zhu Jing , Lu Ting , Pan Likun 
Published: May 2022

AbstractTwo‐dimensional layered material Ti3C2Tx MXene is considered an excellent capacitive deionization (CDI) electrode material due to its two‐dimensional structure and high conductivity. However, the self‐stacking of MXene limits its structural stability and effective ion accommodation in CDI field. In this work, Ti3C2Tx MXene/polypyrrole (PPy) is synthesized by in situ polymerization of conductive PPy on the surface of Ti3C2Tx MXene nanosheets. The hybrid not only effectively alleviates the self‐stacking of MXene to improve the structure stability, but also combines the high conductivity of MXene and the excellent electrochemical capacity of PPy. Therefore, when used as HCDI electrode, Ti3C2Tx MXene/PPy displays an excellent desalination performance including a high desalination capacity of 50.64 mg g−1, a superior desalination rate of 16.5 mg g−1 min−1 and excellent cycling stability. Ti3C2Tx/PPy should be a very promising electrode material for highly efficient desalination.
Iridescent Daytime Radiative Cooling with No Absorption Peaks in the Visible Range
Authors: Ding Zhenmin , Pattelli Lorenzo , Xu Hongbo , Sun Wenhai , Li Xin , Pan Lei , Zhao Jiupeng , Wang Chengyu , Zhang Xiang , Song Ying , Qiu Jun , Li Yao , Yang Ronggui 
Published: May 2022

AbstractCoatings for passive radiative cooling applications must be highly reflected in the solar spectrum, and thus can hardly support any coloration without losing their functionality. In this work, a colorful daytime radiative cooling surface based on structural coloration is reported. A designed radiative cooler with a bioinspired array of truncated SiO2 microcones is manufactured via a self‐assembly method and reactive ion etching. Complemented with a silver reflector, the radiative cooler exhibits broadband iridescent coloration due to the scattering induced by the truncated microcone array while maintaining an average reflectance of 95% in the solar spectrum and a high thermal emissivity (ε) of 0.95, owing to the reduced impedance mismatch provided by the patterned surface at infrared wavelengths, reaching an estimated cooling power of ≈143 W m–2 at an ambient temperature of 25 °C and a measured average temperature drop of 7.1 °C under direct sunlight. This strong cooling performance is attributed to its bioinspired surface pattern, which promotes both the aesthetics and cooling capacity of the daytime radiative cooler.
Advanced Lithium Primary Batteries: Key Materials, Research Progresses and Challenges
Authors: Liu Yan , Su Meng‐Yuan , Gu Zhen‐Yi , Zhang Kai‐Yang , Wang Xiao‐Tong , Du Miao , Guo Jin‐Zhi , Wu Xing‐Long 
Published: May 2022

AbstractIn recent years, with the vigorous development and gradual deployment of new energy vehicles, more attention has been paid to the research on lithium‐ion batteries (LIBs). Compared with the booming LIBs, lithium primary batteries (LPBs) own superiority in specific energy and self‐discharge rate and are usually applied in special fields such as medical implantation, aerospace, and military. Widespread application in special fields also means more stringent requirements for LPBs in terms of energy density, working temperature range and shelf life. Therefore, how to obtain LPBs with high energy density, wide operational temperature range and long storage life is of great importance in future development. In view of the above, this paper reviews the latest research on LPBs in cathode, anode and electrolyte over the years, and puts forward relevant insights for LPBs, along with the intention to explore avenues for the design of LPBs components in the coming decades and promote further development in this field.