bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lower risk of peripheral venous catheter-related bloodstream infection by hand insertion
Authors: Buetti Niccolò , Abbas Mohamed , Pittet Didier , Chraiti Marie-Noëlle , Sauvan Valérie , De Kraker Marlieke E. A. , Boisson Matthieu , Teixeira Daniel , Zingg Walter , Harbarth Stephan 
Published: June 2022

Abstract
Introduction
Little is known about the bloodstream infection (BSI) risk associated with short-term peripheral venous catheters (PVCs) and no large study investigated the insertion site-related risk for PVC-BSI.

Methods
We performed a cohort study at the University of Geneva Hospitals using the prospective hospital-wide BSI surveillance database. We analyzed the association between insertion site and risk of PVC-BSI on the upper extremity using univariable and multivariable marginal Cox models.

Results
Between 2016 and 2020, utilization of 403′206 peripheral venous catheters were prospectively recorded in a 2000-bed hospital consortium with ten sites. Twenty-seven percent of PVC (n = 109′686) were inserted in the hand. After adjustment for confounding factors, hand insertion was associated with a decreased PVC-BSI risk (adjusted hazard ratio [HR] 0.42, 95% CI 0.18–0.98, p = 0.046) compared to more proximal insertion sites. In a sensitivity analysis for PVCs with ≥ 3 days of dwell time, we confirmed a decreased PVC-BSI risk after hand insertion (HR 0.37, 95% CI 0.15–0.93, p = 0.035).

Conclusion
Hand insertion should be considered for reducing PVC infections, especially for catheters with an expected dwell time of more than 2 days.
Multi-platform quantitation of alpha-synuclein human brain proteoforms suggests disease-specific biochemical profiles of synucleinopathies
Authors: Moors Tim E. , Mona Daniel , Luehe Stefan , Duran-Pacheco Gonzalo , Spycher Liz , Mundigl Olaf , Kaluza Klaus , Huber Sylwia , Hug Melanie N. , Kremer Thomas , Ritter Mirko , Dziadek Sebastian , Dernick Gregor , van de Berg Wilma D. J. , Britschgi Markus 
Published: June 2022

AbstractBased on immunostainings and biochemical analyses, certain post-translationally modified alpha-synuclein (aSyn) variants, including C-terminally truncated (CTT) and Serine-129 phosphorylated (pSer129) aSyn, are proposed to be involved in the pathogenesis of synucleinopathies such as Parkinson’s disease with (PDD) and without dementia (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA). However, quantitative information about aSyn proteoforms in the human brain in physiological and different pathological conditions is still limited. To address this, we generated sequential biochemical extracts of the substantia nigra, putamen and hippocampus from 28 donors diagnosed and neuropathologically-confirmed with different synucleinopathies (PD/PDD/DLB/MSA), as well as Alzheimer’s disease, progressive supranuclear palsy, and aged normal subjects. The tissue extracts were used to build a reverse phase array including 65 aSyn antibodies for detection. In this multiplex approach, we observed increased immunoreactivity in donors with synucleinopathies compared to controls in detergent-insoluble fractions, mainly for antibodies against CT aSyn and pSer129 aSyn. In addition, despite of the restricted sample size, clustering analysis suggested disease-specific immunoreactivity signatures in patient groups with different synucleinopathies. We aimed to validate and quantify these findings using newly developed immunoassays towards total, 119 and 122 CTT, and pSer129 aSyn. In line with previous studies, we found that synucleinopathies shared an enrichment of post-translationally modified aSyn in detergent-insoluble fractions compared to the other analyzed groups. Our measurements allowed for a quantitative separation of PDD/DLB patients from other synucleinopathies based on higher detergent-insoluble pSer129 aSyn concentrations in the hippocampus. In addition, we found that MSA stood out due to enrichment of CTT and pSer129 aSyn also in the detergent-soluble fraction of the SN and putamen. Together, our results achieved by multiplexed and quantitative immunoassay-based approaches in human brain extracts of a limited sample set point to disease-specific biochemical aSyn proteoform profiles in distinct neurodegenerative disorders.
Variations of elements, pigments, amino acids and secondary metabolites in Vitis vinifera (L.) cv Garganega after 501 biodynamic treatment
Authors: Malagoli Mario , Sut Stefania , Kumar Gourav , Dall’Acqua Stefano 
Published: June 2022

AbstractBackgroundThere is a need for new approaches in agriculture to improve safety of final products as well as to increase environmental acceptability. In this paper, the biodynamic preparation 501 (horn silica) was sprayed onVitis vinifera(L.) cv Garganega plants in two vineyards located in Veneto region, North-East Italy. Leaf samples were collected on the day of 501-treatment and 11 days later, and berries were sampled at harvest time. Leaves and berries samples were analysed combining targeted and untargeted measurements related to primary metabolism (pigment, element and amino acid contents) and to secondary metabolism. Chlorophyll content in leaves, and amino acid and element (C, N, S) analysis in berries were combined with untargeted UPLC-QTOF metabolomics.ResultsThe discriminant compounds related to the 501-treatment were annotated on the basis of accurate MS and fragmentation and were identified as secondary metabolites, namely phenolic constituents belonging to the shikimate pathway. The level of most of the identified compounds increased in plants treated with 501 preparation.ConclusionsResults highlight the prominent value of the metabolomic approach to elucidate the role of the 501 applications on grapevine secondary metabolism.Graphical Abstract
Fatigue and Recovery Time Course After Female Soccer Matches: A Systematic Review And Meta-analysis
Authors: Goulart Karine Naves Oliveira , Coimbra Cândido Celso , Campos Helton Oliveira , Drummond Lucas Rios , Ogando Pedro Henrique Madureira , Brown Georgia , Couto Bruno Pena , Duffield Rob , Wanner Samuel Penna 
Published: June 2022

Abstract
Background
This study aimed to analyze the extent of fatigue responses after female soccer matches and the ensuing recovery time course of performance, physiological, and perceptual responses.

Methods
Three databases (PubMed, Web of Science, and SPORTDiscus) were searched in October 2020 and updated in November 2021. Studies were included when participants were female soccer players, regardless of their ability level. Further, the intervention was an official soccer match with performance, physiological, or perceptual parameters collected pre- and post-match (immediately, 12 h, 24 h, 48 h, or 72 h-post).

Results
A total of 26 studies (n = 465 players) were included for meta-analysis. Most performance parameters showed some immediate post-match reduction (effect size [ES] = − 0.72 to − 1.80), apart from countermovement jump (CMJ; ES = − 0.04). Reduced CMJ performance occurred at 12 h (ES = − 0.38) and 24 h (ES = − 0.42) and sprint at 48 h post-match (ES = − 0.75). Inflammatory and immunological parameters responded acutely with moderate-to-large increases (ES = 0.58–2.75) immediately post-match. Creatine kinase and lactate dehydrogenase alterations persisted at 72 h post-match (ES = 3.79 and 7.46, respectively). Small-to-moderate effects were observed for increased cortisol (ES = 0.75) and reduced testosterone/cortisol ratio (ES = -0.47) immediately post-match, while negligible to small effects existed for testosterone (ES = 0.14) and estradiol (ES = 0.34). Large effects were observed for perceptual variables, with increased fatigue (ES = 1.79) and reduced vigor (ES = − 0.97) at 12 h post-match, while muscle soreness was increased immediately post (ES = 1.63) and at 24 h post-match (ES = 1.00).

Conclusions
Acute fatigue exists following female soccer matches, and the performance, physiological, and perceptual parameters showed distinctive recovery timelines. Importantly, physical performance was recovered at 72 h post-match, whereas muscle damage markers were still increased at this time point. These timelines should be considered when planning training and match schedules. However, some caution should be advised given the small number of studies available on this population.

Registration
The protocol for this systematic review was pre-registered on the International Prospective Register of Systematic Reviews (PROSPERO, Registration Number: CRD42021237857).
NIR-Mediated drug release and tumor theranostics using melanin-loaded liposomes
Authors: Kim Min Ah. , Lee Chang-Moon 
Published: June 2022

Abstract

Background
Heat generation in a drug delivery carrier by exposure to near-infrared (NIR) light with excellent tissue transmittance is an effective strategy for drug release and tumor therapy. Because liposomes have amphiphilic properties, they are useful as drug carriers. Liposomes are also very suitable for drug delivery strategies using heat generation by NIR laser because lipid bilayers are easily broken by heat. Thermally generated bubbles from liposomes not only induce drug release, but also enable ultrasound imaging.

Methods

Melanin, perfluorohexane (PFH), and 5-fluorouracil (5-FU)-loaded liposomes (melanin@PFH@5-FU-liposomes) that can generate heat and bubble by NIR laser irradiation were prepared by a thin film method. Conversion of light to heat and bubble generation of melanin@PFH@5-FU-liposomes were evaluated using an infrared (IR) thermal imaging camera and an ultrasound imaging system both
in vitro
and
in vivo
. To investigate tumor therapeutic effect, NIR laser of 808 nm was used to irradiate tumor site for 10 min after injecting melanin@PFH@5-FU-liposome into tail veins of CT26-bearing mice.

Results

Melanin@PFH@5-FU-liposomes showed a spherical shape with a size of 209.6 ± 4.3 nm. Upon NIR laser irradiation, melanin@PFH@5-FU-liposomes exhibited effective temperature increase both
in vitro
and
in vivo
. In this regard, temperature increase caused a phase transition of PFH to induce bubble generation dramatically, resulting in effective drug release behavior and ultrasound imaging. The temperature of the tumor site was increased to 52 t and contrast was greatly enhanced during ultrasound imaging due to the generation of bubble. More importantly, tumor growth was effectively inhibited by injection of melanin@PFH@5-FU-liposomes with laser irradiation.

Conclusions
Based on intrinsic photothermal properties of melanin and phase transition properties of PFH, melanin@PFH@5-FU-liposomes exhibited effective heat and bubble generation upon NIR laser irradiation. The elevated temperature induced bubble generation, resulting in contrast enhancement of ultrasound imaging. Melanin@PFH@5-FU-liposomes under NIR laser irradiation induced the death of cancer cells, thereby effectively inhibiting tumor growth. These results suggest that melanin@PFH@5-FU-liposomes can be utilized as a promising agent for photothermal tumor therapy and ultrasound imaging.
Primary headache disorders in epileptic adults
Authors: Osama Ahmed , Orabi Marwa , Yassine Imane , El-Hady Mohamed Elsayed Abd 
Published: June 2022

Abstract
Background
Epilepsy and primary headache disorders are two relatively common neurological disorders and their relationship is still a matter of debate. We aimed to estimate the prevalence and clinical features of primary headache disorders in patients with epilepsy.

Methods
62 subjects aged ≥ 18 years were recruited from the hospital’s neurology outpatient clinic in the period from January to April 2018. The subjects were further divided into two equal groups, epileptics and non-epileptics. They underwent a semi-structured interview including the ILAE 2017 epilepsy classification and the ICHD III-beta criteria for headache. Patients' demographic data and clinical characteristics of epilepsy and headache and temporal relationships between them were assessed. Patients who experienced headaches were grouped based on the type of headaches and on whether their headaches occurred in the pre-ictal, post-ictal or inter-ictal period.

Results
Primary headache disorders were more common in epileptic group (61.3%) than the non-epileptic group (32.2%) (p = 0.021). The tension-type headache was the most common (45.2%) followed by migraine-type headache (12.9%) in the epileptic group. Post-ictal headache was the most common type (29%). Inter-ictal headaches were significantly related to "focal to bilateral tonic–clonic" seizures (p = 0.046). The prevalence of headache among patients on polytherapy (69.2%) was higher than that of patients on monotherapy (52.9%).

Conclusions
In this study, headache was more common in epileptic patients. TTH was the most represented type of headache in patients with epilepsy. Headache occurred in patients with epilepsy most frequently during the post-ictal period.
Economic policy uncertainty and stock returns among OPEC members: evidence from feasible quasi-generalized least squares
Authors: Oyewole Oluwatomisin J. , Adubiagbe Idowu A. , Adekoya Oluwasegun B. 
Published: June 2022

AbstractWe examine the predictive ability of economic policy uncertainty on stock returns of selected OPEC countries. In order to deal with certain statistical properties of the predictors, which include serial correlation, persistence, conditional heteroskedasticity, and endogeneity effects, wse utilize the Feasible Quasi-Generalized Least Squares (FQGLS) estimator in order to obtain accurate forecast estimates. As a precondition for forecast analysis, we conduct the predictability test, which shows that economic policy uncertainty is significant only for five countries, namely Kuwait, Nigeria, Saudi Arabia, United Arab Emirates, and Venezuela. Hence, we proceed with the main forecast analysis for only this set of countries. Our results are twofold. We first account for asymmetries in forecasting stock returns by comparing the forecast performance of the symmetric economic policy uncertainty-based predictive model with its asymmetric variant. On the other hand, we compare the performance of the best model from above with the standard ARFIMA model using an alternative forecast test. In both cases, we find that the asymmetric model yields the most accurate forecast returns for stock returns of the five countries. In essence, neglecting the role of asymmetries in forecasting stock returns can lead to bias results. Our findings are not only robust to different sample sizes (i.e., 50%, and 75%) and different forecast horizons (4, 8, and 12 months) but have important policy implications for policymakers and potential investors.
Digital phenotyping correlations in larger mental health samples: analysis and replication
Authors: Currey Danielle , Torous John 
Published: June 2022

Background
Smartphones can facilitate patients completing surveys and collecting sensor data to gain insight into their mental health conditions. However, the utility of sensor data is still being explored. Prior studies have reported a wide range of correlations between passive data and survey scores.

Aims
To explore correlations in a large data-set collected with the mindLAMP app. Additionally, we explored whether passive data features could be used in models to predict survey results.

Method
Participants were asked to complete daily and weekly mental health surveys. After screening for data quality, our sample included 147 college student participants and 270 weeks of data. We examined correlations between six weekly surveys and 13 metrics derived from passive data features. Finally, we trained logistic regression models to predict survey scores from passive data with and without daily surveys.

Results
Similar to other large studies, our correlations were lower than prior reports from smaller studies. We found that the most useful features came from GPS, call, and sleep duration data. Logistic regression models performed poorly with only passive data, but when daily survey scores were included, performance greatly increased.

Conclusions
Although passive data alone may not provide enough information to predict survey scores, augmenting this data with short daily surveys can improve performance. Therefore, it may be that passive data can be used to refine survey score predictions and clinical utility may be derived from the combination of active and passive data.
Oxytocin receptor gene, post-traumatic stress disorder and dissociation in a community sample of European American women
Authors: Lee Hyunhwa , King Anthony P. , Li Yang , Seng Julia S. 
Published: June 2022

Summary
The aims of this study were: (a) to examine associations of oxytocin receptor gene (OXTR) single nucleotide polymorphisms (SNPs) with post-traumatic stress disorder (PTSD) and dissociative symptoms and (b) to investigate gene–environment (G × E) interaction with childhood maltreatment. Salivary DNA samples from 228 women of European ancestry were analysed. Two SNPs, rs237895 and rs237897, were associated with dissociative symptoms but not PTSD diagnosis. Another SNP (rs2254298) was associated with dissociation when interacting with history of childhood maltreatment. These results contribute to theorising and evidence suggesting that the oxytocin system and its genetics may be associated with risk for dissociation among European American women, including those with maltreatment history. Replication with larger patient samples, including men and other ancestry groups, is needed.
TP53 Sequencing and p53 Immunohistochemistry Predict Outcomes When Bevacizumab Is Added to Frontline Chemotherapy in Endometrial Cancer: An NRG Oncology/Gynecologic Oncology Group Study
Authors: Thiel Kristina W. , Devor Eric J. , Filiaci Virginia L. , Mutch David , Moxley Katherine , Alvarez Secord Angeles , Tewari Krishnansu S. , McDonald Megan E. , Mathews Cara , Cosgrove Casey , Dewdney Summer , Aghajanian Carol , Samuelson Megan I. , Lankes Heather A. , Soslow Robert A. , Leslie Kimberly K. 
Published: June 2022

PURPOSE The status of p53 in a tumor can be inferred by next-generation sequencing (NGS) or by immunohistochemistry (IHC). We examined the association between p53 IHC and sequence and whether p53 IHC alone, or integrated with TP53 NGS, predicts the outcome. METHODS From GOG-86P, a randomized phase II study of chemotherapy combined with either bevacizumab or temsirolimus in advanced endometrial cancer, 213 cases had p53 protein expression data measured by IHC and TP53 NGS data. An analysis was designed to integrate p53 expression by IHC with the presence or absence of a TP53 mutation. These variables were further correlated with progression-free survival (PFS) and overall survival (OS) in the chemotherapy plus bevacizumab arms versus the chemotherapy plus temsirolimus arm. RESULTS In the analysis of p53 IHC, the most striking treatment effect favoring bevacizumab was in cases where p53 was overexpressed (PFS hazard ratio [HR]: 0.46, 95% CI, 0.26 to 0.88; OS HR: 0.31, 95% CI, 0.16 to 0.62). On integrated analysis, patients with TP53 missense mutations and p53 protein overexpression had a similar treatment effect on PFS (HR: 0.41, 95% CI, 0.22 to 0.83) and OS (HR: 0.28, 95% CI, 0.14 to 0.59) favoring bevacizumab plus chemotherapy relative to temsirolimus plus chemotherapy. Concordance between TP53 NGS and p53 IHC was 88%. Concordance was 92% when cases with TP53 mutations and POLE mutations or mismatch repair deficiency were removed. CONCLUSION IHC for p53 alone or when integrated with sequencing for TP53 identifies a specific, high-risk tumor genotype/phenotype for which bevacizumab is particularly beneficial in improving outcomes when combined with chemotherapy.
Pancreatic Cancer Surveillance in Carriers of a Germline CDKN2A Pathogenic Variant: Yield and Outcomes of a 20-Year Prospective Follow-Up
Authors: Klatte Derk C.F. , Boekestijn Bas , Wasser Martin N.J.M. , Feshtali Shahbazi Shirin , Ibrahim Isaura S. , Mieog J. Sven D. , Luelmo Saskia A.C. , Morreau Hans , Potjer Thomas P. , Inderson Akin , Boonstra Jurjen J. , Dekker Friedo W. , Vasen Hans F.A. , van Hooft Jeanin E. , Bonsing Bert A. , van Leerdam Monique E. 
Published: June 2022

PURPOSE Pancreatic cancer surveillance in high-risk individuals may lead to detection of pancreatic ductal adenocarcinoma (PDAC) at an earlier stage and with improved survival. This study evaluated the yield and outcomes of 20 years of prospective surveillance in a large cohort of individuals with germline pathogenic variants (PVs) in CDKN2A. METHODS Prospectively collected data were analyzed from individuals participating in pancreatic cancer surveillance. Surveillance consisted of annual magnetic resonance imaging with magnetic resonance cholangiopancreatography and optional endoscopic ultrasound. RESULTS Three hundred forty-seven germline PV carriers participated in surveillance and were followed for a median of 5.6 (interquartile range 2.3-9.9) years. A total of 36 cases of PDAC were diagnosed in 31 (8.9%) patients at a median age of 60.4 (interquartile range 51.3-64.1) years. The cumulative incidence of primary PDAC was 20.7% by age 70 years. Five carriers (5 of 31; 16.1%) were diagnosed with a second primary PDAC. Thirty (83.3%) of 36 PDACs were considered resectable at the time of imaging. Twelve cases (12 of 36; 33.3%) presented with stage I disease. The median survival after diagnosis of primary PDAC was 26.8 months, and the 5-year survival rate was 32.4% (95% CI, 19.1 to 54.8). Individuals with primary PDAC who underwent resection (22 of 31; 71.0%) had an overall 5-year survival rate of 44.1% (95% CI, 27.2 to 71.3). Nine (2.6%; 9 of 347) individuals underwent surgery for a suspected malignant lesion, which proved to not be PDAC, and this included five lesions with low-grade dysplasia. CONCLUSION This long-term surveillance study demonstrates a high incidence of PDAC in carriers of a PV in CDKN2A. This provides evidence that surveillance in such a high-risk population leads to detection of early-stage PDAC with improved resectability and survival.