bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of genetic diversity among Bambara groundnut lines using SSR markers
Authors: Hlanga Nokuthula , Modi Albert Thembinkosi , Mathew Isack 
Published: May 2022

Abstract
Evaluating available genetic diversity is important for crop improvement especially in neglected crops such as Bambara groundnut. The objective was to characterise genetic variation among 19 Bambara groundnut lines using 20 simple sequence repeat (SSR) markers for initiating Bambara groundnut improvement in South Africa. Genomic DNA was extracted from leaf samples of each genotype and genotyped with diagnostic SSR markers. The SSR fragments were scored numerically as codominant before the genetic parameters were calculated using GenAlex software. The markers exhibited an average polymorphic information content (PIC) of 0.57. Similarly, the observed heterozygosity among the lines was 0.58, showing some level of outcrossing. The first two principal components explained 65.2% of the variation among the genotypes and grouped the genotypes into three clusters. The shortest genetic distance was zero between genotypes Brown and Light brown while Tiganecuru and S19 were farthest related genotypes (0.60). Lines such as IITA686, Cream and Uniswa Red-R that had good performance for multiple agronomic traits occurred within 0.40 genetic distance making them genetically divergent enough for developing breeding populations for Bambara groundnut improvement. The most divergent genotypes with favourable agro-morphological characteristics will be recommended as genetic resources for Bambara improvement and variety development programs.
Efficient Acid Screening Using Covalent-Organic-Framework Membranes with Angstrom-Sized Pore Channels and Built-in Charge-Assisted Hydrogen Bond Donors
Authors: Sun Qi , Meng Qing-Wei , Liu Mingjie , Zuo Xiuhui , Xian Weipeng , Guo Qing , Wang Sai , Yin Hong 
Published: May 2022

Abstract
Acids are extensively used in contemporary industries; however, time-consuming and environmentally unfriendly processes hinder their isolation from wastes containing various ionic species. While membrane technology can overcome these challenges by efficiently extracting analytes of interest, the associated processes typically exhibit inadequate ion-specific selectivity. In this regard, we rationally designed a membrane with uniform angstrom-sized pore channels and built-in charge-assisted hydrogen bond donors that preferentially conduct monobasic acids while exhibiting negligible conductance for other compounds. The ionic selectivity originates from the size screening ability of angstrom-sized channels between protons and other hydrated cations, while the built-in charge-assisted hydrogen bond donor enables the screening of acids by exerting host-guest interactions to varying extent, acting as an anion filter. Furthermore, in contrast to conventional cationic membranes, the strong hydrogen-bonding interactions between the guanidinium moieties and their counter anions resulted in charge transfer from the anions to the membrane skeleton, which switched the effective pore surface charge from positive to negative, thereby facilitating the transport of protons. Ion transport measurements indicated that the resulting membrane exhibited exceptional permeation for protons over other cations and anions of monobasic acids over those of polybasic acids with selectivities up to 4334 and 183, respectively, demonstrating prospects for monobasic acid extraction from waste streams. These findings will aid the design of advanced multifunctional membranes for sophisticated separation.
Effect of different coatings on the structure and performance of three-dimensional ordered macropores La0.7Ce0.3CoO3 catalysts
Authors: Lv Xinyi , Zhang Tianjiao , Cao Jiawei , Wang Yongqiang , Liu Fang , Yin Lihong 
Published: May 2022

Abstract
In this work, monolithic catalysts with single coating Ce0.75Zr0.25O2, Al2O3 and composite coating Ce0.75Zr0.25O2-Al2O3 were prepared by PMMA hard template-excessive impregnation method with 3DOM La0.7Ce0.3CoO3 as the active component and cordierite as the carrier, and characterized by SEM, XRD, BET, H2-TPR and XPS, and the catalytic performance for toluene was tested. The results showed that the active components of 3DOM La0.7Ce0.3CoO3/ Ce0.75Zr0.25O2-Al2O3/ Cordierite catalyst were highly dispersed, forming a complete macroporous structure with the largest specific surface area (10.57 m2·g-1), high surface adsorbed oxygen concentration and good low-temperature reducibility. Its catalytic activity (T50%=103℃, T90%=218℃) for toluene was obviously better than the others. According to the comprehensive analysis, the improvement of the performance of the catalyst may benefit from the excellent physicochemical properties and the synergistic effect between Ce0.75Zr0.25O2-Al2O3 composite coating and active components.
Soft Tissue Phenotype Modification Impacts on Peri-Implant Stability: A Comparative cohort study
Authors: Lin Cho-Ying , Kuo Pe-Yi , Chiu Meng-Yao , Chen Zhao-Zhao , Wang Hom-Lay 
Published: May 2022

Abstract
Objectives
Soft tissue phenotype modification could be performed to maintain peri-implant health. Therefore, the aim of the study was to analyze tissue alteration around implants following soft tissue phenotype modification during implant uncovering surgery.
Materials and methods
Patients had soft tissue phenotype modification (either pouch roll or modified roll technique) during implant second-stage surgery with at least 12-month follow-up were included. Clinical and radiographic parameters including mucosal tissue thickness, recession (REC), keratinized mucosa width (KMW), probing pocket depth (PPD), marginal bone loss (MBL), emergence profile, and emergence angle were extracted from 2-week, 2-month, and 12-month visits after 2nd stage surgery.
Results
Twenty-eight patients with 33 implants fulfilled the inclusion criteria were included. After soft tissue phenotype modification, at 2-week, REC was negatively correlated to mean MTT at mid-buccal site (r=-0.41, p = 0.018) and borderline correlated at mid-lingual site (r=-0.343, p = 0.051). Stable KMW was maintained from 2-week to 12-month with minimal shrinkage rate (3 ~ 14%). MBL change was limited (0.24 ~ 0.47 mm) after STPM. All implants had shallow PPD (≤ 3 mm) with the absence of bleeding on probing. Emergence angle at the mesial side, however, was significantly correlated to surgical techniques, which indicated pouch roll technique would have 6.96 degrees more than modified roll technique (p = 0.024)
Conclusions
Soft tissue phenotype modification, either pouch roll or modified roll technique, during uncovering surgery resulted in favorable clinical outcomes. Thin mucosal tissue thickness and pouch roll technique are the factors related to more recession at 2 weeks. Pouch roll technique could influence the restorative design by having a wide emergence angle at the mesial side.
Clinical relevance:
Modified and pouch roll techniques during uncovering surgery were viable methods to yield favorable peri-implant health, while the preciseness of pouch roll technique was required to avoid mucosal recession and inadequate restorative design.
Synthetic double inversion recovery imaging in brain MRI: Quantitative evaluation and feasibility of synthetic MRI and a comparison with conventional double inversion recovery and fluid-attenuated inversion recovery sequences
Authors: Zorigt Odgerel , Nakajima Takahito , Kumasaka Yuka , Jingu Akiko , Tsushima Yoshito 
Published: May 2022

Abstract
Background and purpose: Synthetic MR imaging (SyMRI) allows the reconstruction of various contrast images, including double inversion recovery (DIR), from a single scan. This study aimed to investigate the advantages of SyMRI by comparing synthetic DIR images with synthetic T2-weighted fluid-attenuated inversion recovery (T2W-FLAIR) and conventional DIR images. Materials and Methods: We retrospectively reviewed the imaging data of 100 consecutive patients who underwent conventional brain MRI between December 2018 and March 2019. Synthetic DIR, T2W-FLAIR, T1-weighted, and phase-sensitive inversion recovery (PSIR) images were generated from SyMRI data. For synthetic DIR, the two inversion times required to suppress white matter and cerebrospinal fluid (CSF) were manually determined by two radiologists. Quantitative analysis was performed by manually tracing the region of interest (ROI) at the sites of the lesion, white matter, and CSF. Synthetic DIR, synthetic T2W-FLAIR, and conventional DIR images were compared on the basis of using the gray matter-to-white matter, lesion-to-white matter, and lesion-to-CSF contrast-to-noise ratios. Results: The two radiologists showed no differences in setting inversion time (TI) values, and their evaluations showed excellent interobserver agreement. The mean signal intensities obtained with synthetic DIR were significantly higher than those obtained with synthetic T2W-FLAIR and conventional DIR. Conclusion: Synthetic DIR images showed a higher contrast than synthetic T2WFLAIR and conventional DIR images.
Comparison between the effect of the information–motivation–behavioral (IMB) model and psychoeducational counseling on sexual satisfaction and contraception method used under the coercion of the spouse in Iranian women: A Randomized, Clinical Trial
Authors: Zarbaf Alieh , Ahmadi Atefeh , Rafati Elahe , Ghorbani Faezeh , pour Masumeh Ghazanfar , Alidousti Katayoun 
Published: May 2022

Abstract
Background: Women play an essential role in maintaining the family's health, and family planning is part of women's and families' health. The couple's mutual understanding of family planning methods is essential in selecting a contraception method. Acceptance of and satisfaction with different contraception methods can impact sexual satisfaction. The present study aimed to compare the effect of the information-motivation-behavioral (IMB) model and psychoeducational counseling on sexual satisfaction and contraception methods of women referring to healthcenters in Kerman.Methods: This trial was conducted on 81 women aged 18–45 who came to Kerman health centers from 2021 to 2022. Participants were randomly divided into three groups of 27 people (control, psychoeducational counseling, IMB method). Due to the COVID-19 outbreak, three online counseling sessions were held for the psychoeducational counseling intervention group, and four online counseling sessions were held for the IMB group. The control group received routine care from a midwife. SPSS software was used for data analysis using nonparametric Friedman and Kruskal-Wallis tests. Results: The mean age of participants was 32.59 ± 7.04, and the majority had university degrees and were homemakers. The mean sexual satisfaction score significantly increased immediately after the intervention and one month later in the two interventional groups (P <0.001). Changes in contraception methods after intervention were significant in the psychoeducational group (P = 0.028)Conclusion: The results indicate the positive impact of psychological counseling on women's sexual satisfaction and changing the contraception method. The results also showed that the IMB method positively impacted women's sexual satisfaction but had no impact on changing the contraceptive method.
Predictive value of perioperative monocyte counts for bacteremia and 90-day all-cause mortality in patients with severe burn
Authors: He Qiulan , Chen Caiyun , Gao Shaowei , Yang Lu , Huang Runcheng , Qin Ying , Huang Wenqi 
Published: May 2022

Abstract
Objective To explore the predictive value of changes in monocytes (M), lymphocytes (L), white blood cell-to-platelet ratio (WPR), neutrophil-to-lymphocyte ratio (NLR) in peripheral blood and changes in temperature (T-37) for bacteremia and 90-day mortality in patients with severe burns during the first perioperative period.Methods Data from 169 patients treated at the First Affiliated Hospital of Sun Yat-sen University from 2011 to 2020 were retrospectively analyzed. The patients were divided into a bacteremia group and a control group based upon blood culture. Data on M, L, NLR, WPR and T-37 were collected at the day before the first surgery (0), the first day after the first surgery (1) and the third day after the first surgery (3). Independent risk factors for bacteremia and 90-day mortality were identified. The risk prediction models were established with multivariate logistic regression analysis and externally validated in another cohort of 191 patients with severe burns.Results The 90-day mortality rate was 21.3% (36/169), which was significantly higher in the Bac group than in the con group (42.8% vs. 10.6%, P<0.01). WPR0, M0, WPR1, and T3-37 were significantly higher in the Bac group than in the con group (P<0.01), whereas M1 and M3 were significantly lower in the Bac group than in the con group (P<0.01). Multivariate regression analysis showed that SOFA0, WPR0, M3, and T3-37 were independently associated with bacteremia. The prediction model for bacteremia Xbac = 0.1809 × SOFA0 + 6.532 × WPR0-1.171 × M3 + 0.6987 × ⎮T3-37⎮- 2.297. TBSAB, SOFA0, and ∆M (M0-M3) were independently correlated with 90-day mortality. The risk prediction model X90d-m= 0.055 × TBSAB + 0.301×SOFA0+1.508 × ∆M) - 7.196. External validation of the prediction models suggested that the specificity, sensitivity, and AUC were 90.7%, 62.5% and 0.797, respectively, for Xbac and 69.2%, 90.0% and 0.873, respectively, for X90d-m.Conclusion ∆M and WPR0 were independent risk factors for bacteremia and 90-day mortality in patients with severe burns. The predictive models could inform clinical antimicrobial judgment and prognostication.
Does energy intensity matter in the nexus between energy consumption and economic growth regarding capital-energy substitution?
Authors: Aydin Celil , Onay Reyhan Demir , Sahin Ismail 
Published: May 2022

Abstract
This article investigates how the non-linear connection between energy consumption and economic development is influenced by energy intensity level in the context of energy-capital substitution. We firstly analyze the substitutability/complementarity between energy and capital by estimating VES production function within the standard Solow growth model framework for 58 countries over the period of 1975–2017. The selected countries are classified into four groups according to their relative energy intensity levels and their accessibility to energy. The estimation findings reveal that energy and capital are complements in the final output for each country group. Hence, in this paper as further analysis, the study examines whether or not energy consumption always fosters economic growth. We investigate the non-linear link between energy consumption and economic development by constructing a Panel Smooth Transition Regression (PSTR) model for each country group and looking at the impact of energy intensity in this relationship. The empirical results provide that for each country group, there is a threshold level for energy intensity. Regardless of whether a country is a net energy exporter or net energy importer, it needs to use energy efficiently and not exceed the ideal energy intensity level in both production and consumption to maintain long-term economic growth.
Impact of COVID-19 Vaccination on the Menstrual Cycle: A Cross-Sectional Study from Karachi, Pakistan
Authors: Sualeh Muhammad , Uddin Muhammad Roohan , Junaid Natalia , Khan Muneeba , Pario Anam 
Published: May 2022

Abstract
Background The COVID-19 disease triggered a worldwide health catastrophe. To deal with this deadly situation multiple vaccines were developed and a mass immunization program started globally. However, vaccine hesitancy was seen especially among women of reproductive age, having concerns that the vaccine might affect their menstrual cycle. This study investigated the link between COVID vaccination and menstrual abnormalities. It is essential for us to understand the effects of vaccines on menstruation as menstrual distress can have effects on everyday life, and mental and reproductive health. MethodsA cross-sectional study was performed using self-administered online forms to collect data from all over Karachi. The sample included 384 females aged 18 years and above. The data was collected from November 2021 to February 2022. ResultsMajority of the participants were aged 21 and had a normal BMI. Most were moderately stressed (n = 245) with 146 reporting menstrual changes post-vaccination. The difference between the post-vaccine menstruation affected (n = 146) and the unaffected cohort (n = 238) was significant. Other factors which likely contributed to the post-vaccine menstrual changes included PSS score, strenuous physical activity, and the pre-vaccine menstrual flow.ConclusionsIn conclusion, there is no denying that existing data is inadequate, which is one of the grounds for vaccination apprehension, particularly among menstruating women. To minimize this hesitation, the spread of disinformation about the vaccine's influence on the menstrual cycle must be avoided. Because such abnormalities may lower their overall quality of life. Policy-based interventions should also be implemented to document any menstruation-related side effects.
A novel collaborative optimization assembly process method for multi-performance of aeroengine rotors
Authors: Li Tao , Wen Zhenhua , Zhao Binbin , Sun Qingchao 
Published: May 2022

Abstract
In the assembly process of aeroengine, the aeroengines have been rejected at pass off test for high vibration of high pressure rotor under the condition of meeting the geometric accuracy. Repeated disassembly and trial assembly will lead to interface damage, which will affect the safety and reliability of the engine. Therefore, a multi-objective collaborative optimization process method for geometric accuracy and dynamic performance is put forward based on the practical engineering problems. In the proposed method, the primal problem is firstly reveals the transfer mechanism of coaxiality and unbalance of rotor system, and the prediction model of the coaxiality and unbalance of the rotor system was established. Then, combined with the high-precision reduced order model of aeroengine high-pressure combined rotor, the system vibration responses under different assembly states are obtained. Finally, a multi-objective collaborative optimization assembly process method considering coaxiality, unbalance and vibration response is obtained. The results show that the collaborative optimization process method considering assembly accuracy and dynamic performance is the optimal assembly strategy, which can reduce the vibration amplitude of the key nodes of the rotor system while ensuring high assembly accuracy, and achieve the low-level vibration of the rotor system.
The Lack of Fam83h Mediated Reduction of Wnt/Β-Catenin Signaling Pathway and Expression Levels of Dental Mineralization Genes
Authors: Nasseri Sherko , Parsa Sara , Vahabzadeh Zakaria , Khadem-Erfan Mohammad Bagher , Nikkhoo Bahram , Baban Babak , Mirzaie Sako , Ebadifar Asghar , Fathi Fardin 
Published: May 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Fabrication and Characterization of Plasmonic Au Nanoparticles on ITO coated glass sheets
Authors: Verma Anuradha , Saxena Sakshi , Biswas Neeraj Kumar , Srivas Anupam , Singh Udai Bhan , Khan Saif. A. , Avasthi Devesh Kumar , Dass Sahab 
Published: May 2022

Abstract
Noble metal nanoparticles are the topic of profound interest on account of their exceptional optical properties contrary to their bulk counterparts. We report here the fabrication of sputter-deposited Au nanoparticles on ITO (Sn doped Indium Oxide) coated glass substrate. The effect of varying annealing parameters viz. time and temperature in conjunction with varying thickness parameters have been thoroughly investigated. Prepared thin films were characterized using FE-SEM (Field emission scanning electron microscope), RBS (Rutherford backscattering) measurement, and optical absorbance spectroscopy. The role of annealing temperature and time duration on the evolution of Au nanoparticles is discussed. At low temperature, film dewetting has been observed while at high temperature and longer duration of annealing leads to particle dissolution for the greater thickness of 5 nm and 8 nm in the case of Au thin films. According to the study, 5 nm thickness Au thin film which was annealed at 500oC for 90 min exhibited maximum SPR (surface plasmon resonance) effect.
Fe2+-induced surface plasmon-catalyzed reduction reaction for the detection of Fe2+
Authors: Sun Ye , Xu Guangda , Wang Yue , song Peng , Zhang Yao , Xia Lixin 
Published: May 2022

Abstract
Herein, we developed a concise, time-efficient, and high selective assay for detecting Fe2+ through its triggered surface plasmon-catalyzed reduction reaction of p-nitrothiophenol (PNTP) to p,p′-dimercaptoazobenzene (DMAB) based on surface-enhanced Raman scattering (SERS) spectroscopy. When Fe2+ was added to the PNTP-AuNPs system, the appearance of three characteristic peaks at 1142, 1392, and 1140 cm− 1 attributed to DMAB demonstrated that Fe2+ induced the catalytic coupling reaction of PNTP. The Raman intensity ratio of the peak at 1142 cm− 1 to the peak at 1336 cm− 1 and the concentration of Fe2+ presented a good linear response from 10 to 100 µM with a limit of detection (LOD) of 0.35 µM. Besides, the probable reaction process has been put forward. More importantly, the entire detection process can be completed within 2 min and was used successfully for the detection of Fe2+ in river water.
Neotropical Monogenoidea 65. Cosmetocleithrum infinitus sp. n. (Monogenoidea: Dactylogyridae) parasite of the gills of Anadoras grypus (Siluriformes: Doradidae) from the Amazonas river, in Loreto, Peru
Authors: Morey Germán Augusto Murrieta , Rojas Carlos Alfredo Tuesta , Cachique José Carlos Zumaeta 
Published: May 2022

Abstract
Among Siluriformes, fishes of the family Doradidae harbor dactylogyrids from Cosmetocleithrum Kritsky, Thatcher and Boeger, 1986. Anadoras grypus (Cope, 1872) (Siluriformes, Doradidae) locally named in Peru as “rego-rego” hosts a new species of Cosmetocleithrum described herein as Cosmetocleithrum infinitus sp. n. The male copulatory organ (MCO) and vagina of the new species reveals its morphological uniqueness among members of Cosmetocleithrum. The MCO in the new species is an elongate, sclerotized sinuous tube, with numerous and uncountable coils with counterclockwise orientation; the accessory piece is sclerotized, comprising a kind of cone, with a large opening at its base, with a branch in the posterior middle part and with pointed ends. The vagina is a sclerotized long, winding, coiled tube with a cone-shaped opening.