bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endosperm and Maternal-specific expression ofEIN2in the endosperm affects endosperm cellularization and seed size inArabidopsis
Authors: Ando Atsumi , Kirkbride Ryan C , Qiao Hong , Chen Z Jeffrey 
Published: October 2022

AbstractSeed size is related to plant evolution and crop yield and is affected by genetic mutations, imprinting, and genome dosage. Imprinting is a widespread epigenetic phenomenon in mammals and flowering plants. ETHYLENE INSENSITIVE2 (EIN2) encodes a membrane protein that links the ethylene perception to transcriptional regulation. Interestingly, during seed development EIN2 is maternally expressed in Arabidopsis and maize, but the role of EIN2 in seed development is unknown. Here, we show that EIN2 is expressed specifically in the endosperm, and the maternal-specific EIN2 expression affects temporal regulation of endosperm cellularization. As a result, seed size increases in the genetic cross using the ein2 mutant as the maternal parent or in the ein2 mutant. The maternal-specific expression of EIN2 in the endosperm is controlled by DNA methylation but not by H3K27me3 or by ethylene and several ethylene pathway genes tested. RNA-seq analysis in the endosperm isolated by laser-capture microdissection show upregulation of many endosperm-expressed genes such as AGAMOUS-LIKEs (AGLs) in the ein2 mutant or when the maternal EIN2 allele is not expressed. EIN2 does not interact with DNA and may act through ETHYLENE INSENSITIVE3 (EIN3), a DNA-binding protein present in sporophytic tissues, to activate target genes like AGLs, which in turn mediate temporal regulation of endosperm cellularization and seed size. These results provide mechanistic insights into endosperm and maternal-specific expression of EIN2 on endosperm cellularization and seed development, which could help improve seed production in plants and crops.
Below the Surface of Clinical Ethics
Authors: Parker J Clint 
Published: October 2022

AbstractOften lurking below the surface of many clinical ethical issues are questions regarding background metaphysical, epistemological, meta-ethical, and political beliefs. In this issue, authors critically examine the effects of background beliefs on conscientious objection, explore ethical issues through the lenses of particular theoretical approaches like pragmatism and intersectional theory, rigorously explore the basic concepts at play within the patient safety movement, offer new theoretical approaches to old problems involving decision making for patients with dementia, explicate and explore the problems and promises of machine learning in medicine, and offer us a non-rights-based argument for the just distribution of healthcare resources.
Strategic analysis of the drought resilience of water supply systems
Authors: Murgatroyd Anna , Gavin Helen , Becher Olivia , Coxon Gemma , Hunt Doug , Fallon Emily , Wilson Jonny , Cuceloglu Gokhan , Hall Jim W. 
Published: October 2022

Severe droughts can result in shortages of water supplies, with widespread social and economic consequences. Here we use a coupled simulation model to assess the reliability of public water supplies in England, in the context of changing scenarios of water demand, water regulation and climate change. The coupled simulation model combines climate simulations, a national-scale hydrological model and a national-scale water resource systems model to demonstrate how extreme meteorological droughts translate into hydrological droughts and water shortages for water users. We use this model to explore the effectiveness of strategic water resource options that are being planned in England to secure water supplies to most of England's population up to a drought return period of 1 in 500 years. We conclude that it is possible to achieve a 1-in-500-years standard in locations where strategic resource options are used, while also reducing water abstraction to restore the aquatic environment. However, the target will be easier to achieve if effective steps are also taken to reduce water demand.This article is part of the Royal Society Science+ meeting issue ‘Drought risk in the Anthropocene’.
Understanding the transgression of global and regional freshwater planetary boundaries
Authors: Pastor A. V. , Biemans H. , Franssen W. , Gerten D. , Hoff H. , Ludwig F. , Kabat P. 
Published: October 2022

Freshwater ecosystems have been degraded due to intensive freshwater abstraction. Therefore, environmental flow requirements (EFRs) methods have been proposed to maintain healthy rivers and/or restore river flows. In this study, we used the Variable Monthly Flow (VMF) method to calculate the transgression of freshwater planetary boundaries: (1) natural deficits in which flow does not meet EFRs due to climate variability, and (2) anthropogenic deficits caused by water abstractions. The novelty is that we calculated spatially and cumulative monthly water deficits by river types including the frequency, magnitude and causes of environmental flow (EF) deficits (climatic and/or anthropogenic). Water deficit was found to be a regional rather than a global concern (less than 5% of total discharge). The results show that, from 1960 to 2000, perennial rivers with low flow alteration, such as the Amazon, had an EF deficit of 2–12% of the total discharge, and that the climate deficit was responsible for up to 75% of the total deficit. In rivers with high seasonality and high water abstractions such as the Indus, the total deficit represents up to 130% of its total discharge, 85% of which is due to withdrawals. We highlight the need to allocate water to humans and ecosystems sustainably.
This article is part of the Royal Society Science+ meeting issue ‘Drought risk in the Anthropocene’.
PALMO: a comprehensive platform for analyzing longitudinal multi-omics data
Authors: Vasaikar Suhas V. , Savage Adam K. , Gong Qiuyu , Swanson Elliott , Talla Aarthi , Lord Cara , Heubeck Alexander T , Reading Julian , Graybuck Lucas T. , Meijer Paul , Torgerson Troy R. , Skene Peter J. , Bumol Thomas F. , Li Xiao-jun 
Published: October 2022

AbstractLongitudinal bulk and single-cell omics data is increasingly generated for biological and clinical research but is challenging to analyze due to its many intrinsic types of variations. We present PALMO (https://github.com/aifimmunology/PALMO), a platform that contains five analytical modules to examine longitudinal bulk and single-cell multi-omics data from multiple perspectives, including decomposition of sources of variations within the data, collection of stable or variable features across timepoints and participants, identification of up- or down-regulated markers across timepoints of individual participants, and investigation on samples of same participants for possible outlier events. We tested PALMO performance on a complex longitudinal multi-omics dataset of five data modalities on the same samples and six external datasets of diverse background. Both PALMO and our longitudinal multi-omics dataset can be valuable resources to the scientific community.
Predicting murine age across tissues and cell types using single cell transcriptome data
Authors: Neumann Janis Frederick , Leote Ana Carolina , Liersch Meike , Beyer Andreas 
Published: October 2022

AbstractMolecular aging clocks utilize high-dimensional profiling data to predict the chronological or biological age of individuals. While this approach has proven successful across a wide range of species and tissues, the potential of using single-cell molecular profiling data for age prediction remains to be fully explored. Here, we demonstrate that aging clocks based on single-cell RNA-sequencing (scRNA-seq) data enable studying aging effects for different cell types in the same organ and for similar cell types across organs. We utilize mouse single-cell RNA-Seq data to train molecular aging clocks that distinguish between cells of young and old mice using two models: a first model trained specifically to predict the age of B cells and a second one predicting age across 70 cell types from 14 tissues.We evaluated Elastic Net regression and two tree-based machine learning methods, Random Forest and XGBoost, as well as three distinct methods of transforming the measured gene expression values. Our models proved to be transferable to independent individuals and tissues that were not used for model training, reaching an accuracy of over 90%. A single-cell molecular aging clock trained on B cells from the spleen was capable of correctly classifying the age of almost 95% of all B cells in different organs. This finding suggests common molecular aging processes for B cells, independent of their site of residence. Further, our aging models identified several aging markers involved in translation and formation of the cytoskeleton, suggesting that these fundamental cellular processes are affected by aging independent of the cell type. Beyond showing that it is possible to train highly accurate and transferable models of aging on single-cell transcriptomics data, our work opens up the possibility of studying global as well as cell-type-specific effects of age on the molecular state of a cell.Graphical Abstract
The priming effect of rewarding brain stimulation persists following blockade of D2-like dopamine receptors
Authors: Evangelista Czarina , Mehrez Norhan , Brake Wayne G. , Shizgal Peter 
Published: October 2022

Withdrawal statementThe authors have withdrawn their manuscript because its contents have now been merged into BIORXIV/2022/512941v3. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.
Complexes of 2,4,6-trihydroxybenzoic acid: effects of intramolecular hydrogen bonding on ligand geometry and metal binding modes
Authors: Abrahams Brendan F. , Commons Christopher J. , Hudson Timothy A. , Sanchez Arlt Robin , Ahl Rion , Carajias Eirene D. , Chan Jason W. K. , Guo Zhihao , Hill Renee E. , McGinty Alice , Peters Neale L. , Poon Joshua Y. P. , Qu Jingqi , Qu Jinglin , Rochette Emily E. , Walkear Catherine , Wang Hanlin , Wu Holly , Xu Chang , Zhang Jingyuan 
Published: October 2022

This article describes a series of more than 20 new compounds formed by the combination of 2,4,6-trihydroxybenzoic acid (H4thba) with metal ions in the presence of a base, with structures that include discrete molecular units, chains, and two- and three-dimensional networks. As a result of the presence of two ortho-hydroxy groups, H4thba is a relatively strong acid (pK
a1 = 1.68). The carboxylate group in H3thba− is therefore considerably less basic than most carboxylates with intramolecular hydrogen bonds, conferring a rigid planar geometry upon the anion. These characteristics of H3thba− significantly impact upon the way it interacts with metal ions. In s-block metal compounds, where the interaction of the metal centres with the carboxylate O atoms is essentially ionic, the anion bonds to up to three metal centres via a variety of binding modes. In cases where the metal ion is able to form directional coordinate bonds, however, the carboxylate group tends to bond in a monodentate mode, interacting with just one metal centre in the syn mode. A dominant influence on the structures of the complexes seems to be the face-to-face stacking of the aromatic rings, which creates networks containing layers of metal–oxygen polyhedra that participate in hydrogen bonding. This investigation was undertaken, in part, by a group of secondary school students as an educational exercise designed to introduce school students to the technique of single-crystal X-ray diffraction and enhance their understanding of primary and secondary bonding.
Structural networks and dyadic negotiations in tourism destination ecosystems
Authors: Ness Håvard , Aarstad Jarle , Haugland Sven Arne 
Published: October 2022

Purpose
This study aims to investigate how and to what extent structural network properties affect dyadic negotiation behavior in tourism destination ecosystems. Specifically, this study addresses negotiation behavior in terms of problem-solving and contending, because these two key strategies reflect the integrative and distributive aspects of dyadic interactions.

Design/methodology/approach
This study relies on network data and dyadic survey data from nine mountain tourism destinations in Southeastern Norway. The structural network properties the authors research are triadic closure – the extent to which a dyad has common ties to other actors – and structural equivalence – the similarities in networking patterns that capture firms’ competition for similar resources. In addition, the authors also study a possible effect of relationship duration on negotiation behavior.

Findings
Triadic closure and relationship duration have positive effects on problem-solving, and structural equivalence tends to decrease problem-solving, although the effect is inconsistent; none of these three independent variables was found to affect contending negotiation behavior.

Research limitations/implications
This study shows that a dyad’s structural network embeddedness has implications for negotiation behavior. Further research is encouraged to develop this theoretical perspective.

Originality/value
This study is a pioneering investigation of how structural network properties affect dyadic negotiation behavior in ongoing coproducing relationships in real-world destination ecosystems.
Is online collaboration process suitable for digital youth organization? A design approach
Authors: Cheng Xusen , Liu Wanxin , Li Yuanyuan 
Published: October 2022

PurposeCollaboration is significant but difficult for the development of youth organizations, this research aims to explore whether the online collaboration process is suitable for youth organizations' collaboration and improve their effectiveness and efficiency.Design/methodology/approachThis research has applied a design approach using the collaboration engineering method, to design an online collaboration process for youth organizations to improve their effectiveness and efficiency. Using a self-developed group support systems (GSS) tool, the authors have tested the new collaboration process through an experiment among four youth organizations and conducted a survey afterwards.FindingsThe new process improves the collaboration effectiveness and efficiency. The research also identifies the detailed relationships among influencing factors in the online collaboration process.Originality/valueThere is little research in the context of computer mediated youth organization collaboration. This research designs an online collaboration process for the effective and efficient collaboration of youth organizations and has it tested among representative youth organizations, providing practical instructions for digital youth organization collaboration in the context of global pandemic.
Listing product benefits on an extra affixed label: effects on claim credibility and product evaluation
Authors: Ku Hsuan-Hsuan , Chen Pei-Ting 
Published: October 2022

Purpose
To heighten shopper interest, fast moving consumer goods marketers often attach supplementary labels to the package front to promote product benefits. This study aims to use claim credibility as the foundation for investigating how an extra affixed label that addresses product benefits impacts consumer evaluation, as well as identifying important factors that might moderate the resulting responses.

Design/methodology/approach
Three between-subjects experiments examine how claim credibility mediates the influence of extra affixed labels on product evaluation (Study 1). They also test whether the impact on consumer responses of extra affixed labels, with emphasis on the same vs different benefits as those printed on the front of a package (Study 2.1) or with a high or low relevance between their claimed benefits and the front-of-package stated ingredients (Study 2.2), is dependent upon individuals’ need for cognition.

Findings
Results show the power of extra affixed labels in improving product evaluation. Claim credibility mediated the observed effects of extra affixed labeling. Yet, the favorable effects of extra affixed labels for individuals high in need for cognition is diminished when expressed in a different (vs same) claim from those printed on the package front or the claim about product benefits is low (vs high) relevance to the declared ingredients. The reverse holds true for those low in need for cognition.

Originality/value
This study advances knowledge on the effects of extra affixed label claims on product evaluation.
Design and development of an environmentally controlled enclosure for a commercial 3D printer
Authors: Gonzalez Lugo Carlos A. , Caputo Dylan Scott , Hutchinson Michael J. , Fouladi Kamran , Eslami Babak 
Published: October 2022

Purpose
The purpose of this study is to design and develop an environmentally controlled enclosure for commercial three-dimensional (3D) printers.

Design/methodology/approach
Computational fluid dynamics (CFD) simulations and experimental testing investigated various designs for environmentally controlled enclosures. CFD simulations provided the necessary information to select the optimal and feasible design, whereas experimental testing validated the CFD simulation results. An environmentally controlled environment allowed test samples to be printed at several relative humidity (RH) settings (20% RH, 50% RH and 80% RH). The test samples were characterized at both the macro and micro scales. The macroscale characterization was conducted using the static tensile testing procedure, while the microscale polymer material properties were determined using atomic force microscopy.

Findings
An environmentally controlled enclosure was designed and built to produce airflow in the print region with an average RH uniformity of over 0.70. Three batches of ASTM D638 standard test samples were printed at 20% RH (low RH), 50% RH (mid RH) and 80% RH (high RH). Macroscale characterization showed that the samples printed at lower humidity had statistically significantly higher tangent modulus, ultimate tensile strength and rupture strength. atomic force microscopy studies have also verified these results at the microscale and nanoscale. These studies also showed that a high humidity environment interacts with melted polylactic acid, causing additional surface roughness that reduces the strength of 3D-printed parts.

Originality/value
There is a need for stronger and higher-quality 3D-printed parts in the additive manufacturing (AM) market. This study fulfills that need by designing and developing an environmentally controlled add-on enclosure for the AM market.
How potential and realized absorptive capacity increased ability to innovate: the moderating role of structural ambidexterity
Authors: Elidjen Elidjen , Pertiwi Asri , Mursitama Tirta Nugraha , Beng Jap Tji 
Published: October 2022

Purpose
Digital start-ups have limited resources. With the demands of rapid growth, digital start-ups need to rely on their ability to explore external knowledge and exploit it into swift innovation. Developing absorptive capacity is an alternative to overcome this difficulty. This study aims to demonstrate how the potential and realized an increase in absorptive capacity enables organizations to innovate moderated by structural ambidexterity. Empirical evidence places more emphasis on the impact of absorptive capacity on innovation but still leaves the “black-box” question of innovation and how potential absorptive capacity (PACAP) can achieve realized absorptive capacity (RACAP).

Design/methodology/approach
This study tests, with a structural equation model, samples collected from 143 digital start-ups in Indonesia.

Findings
The finding of this study suggests that PACAP influences the ability to innovate only if RACAP mediates it and structural ambidexterity positively moderates the relationship between these two variables.

Research limitations/implications
First, this study uses digital start-up organizations as respondents. Second, this study explores the role of the structural ambidexterity that moderates the relationship between PACAP and RACAP manifested in digital start-ups organizations that are identical to temporary companies with limited resources. Third, digital start-ups have a fast-growth life cycle, unlike regular companies. Finally, the validated scale is based on data collected entirely from digital start-ups located in Indonesia, which may limit the generalizability of the findings to other industry contexts.

Practical implications
Start-ups suffer from the ability to innovate that increases their propensity to fail. They overcome this failure by increasing the absorptive capacity of the founding team to improve their ability to innovate. Because of limited resources available at digital start-ups, the flexibility of their management style can overcome these barriers, allowing the pursuit of both knowledge exploration and exploitation in a balanced way.

Originality/value
Most of the studies explained that the ability to innovate comes from absorptive capacity. In fact, they do not explore PACAP and RACAP and their relationships. Moreover, the studies also indicated that the contextual ambidexterity moderated PACAP and RACAP. Meanwhile, digital start-ups in this study revealed that structural ambidexterity with two dimensions, i.e. shared value, and behavioral integration, enables and positively moderates the relationship between PACAP and RACAP.
Challenges in diagnosis and management of delirium in Lewy body disease
Authors: Richardson Sarah , Lawson Rachael A. , Price Annabel , Taylor John‐Paul 
Published: October 2022

AbstractBackgroundDelirium is an acute onset and fluctuating impairment of cognition, attention and arousal, often precipitated by acute illness. Lewy body disease (LBD) is an umbrella term for a range of clinical conditions, including Parkinson's disease dementia (PDD) and dementia with Lewy bodies (DLB). People living with LBD seem to be more susceptible to delirium than those with other subtypes of dementia.AimTo describe the challenges in clinical diagnosis and management of LBD.MethodsA systematic review of published literature on diagnosis and management of delirium in LBD.ResultsDelirium is particularly challenging to diagnose in LBD as many of the clinical characteristics which define delirium such as inattention, fluctuating arousal, complex visual hallucinations and delusions, are also common to LBD. Distinguishing delirium from LBD can be very difficult clinically especially in the prodromal stages. Both under and over diagnosis of delirium, and under and over treatment of the symptoms have the potential to compromise the care and safety of people with a diagnosed or undiagnosed LBD.Clinicians are currently working with an extremely limited set of evidence‐based management options for those with delirium in the context of a LBD diagnosis. For patients with LBD and their families this is an area of clinical practice that needs focused research.