bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of GRACE Risk Score with Angiographic Severity of Coronary Artery Disease in Patients with Non ST-elevation Acute Coronary Syndrome
Authors: Rahman Md Estakur , Mohammad Safiuddin - , Habib SM Ahsan , Ahmed Chaudhury Meshkat , Banerjee Sajal Krishna 
Published: June 2022

Background: Due to the wide spectrum of risk for death and recurrent events among patients with NSTE-ACS, management guidelines emphasise the importance of early risk stratification. In addition to prognostic assessment, predicting the anatomical extension of coronary artery disease is potentially useful for clinical decision. The aim of the study is to determine whether the GRACE risk score correlates with the angiographic extent and severity of coronary artery disease in patients with non ST-elevation ACS
Methods: A total of 50 patients with non-ST-elevation acute coronary syndrome were enrolled to the study. Based on the GRACE risk score classification system, the patients were divided into low (<108), intermediate (109-140), and high (>140) risk groups. All patients underwent coronary angiography within five days after admission. The severity of the coronary artery disease was assessed by Gensini score. Relation between Grace score and Gensini score was evaluated.
Results: Mean Gensini score were 12.20 ± 13.60, 34.52 ± 13.50 and 48.41 ± 14.56 in low group, intermediate group and high GRACE risk group respectively and the difference of mean Gensini score was statistically significant (p<0.001). A GRACE score of 135 was identified as the optimal cut-off to predict severe CAD (sensitivity = 82.4% and specificity = 75.8%). In our study correlation co-efficient between GRACE risk score and Gensini score was r=0.66 (p<0.001).
Conclusion: The study demonstrates that the GRACE risk score has significant positive correlation with coronary artery stenosis in patients with non-ST-elevation acute coronary syndrome.
University Heart Journal Vol. 17, No. 1, Jan 2021; 38-41
Antiplatelet Therapy in Patients with Acute Coronary Syndrome: An Update
Authors: Bashar Adnan , Hoque Md Harisul , Chowdhury MSI Tipu , Hasan ATM Iqbal , Ahmed Khurshed 
Published: June 2022

Antiplatelet therapy is the cornerstone in the treatment of acute coronary syndrome. Aspirin is the most widely used antiplatelet agent followed by P2Y12 inhibitors. Glycoprotein (GP) IIb-IIIa antagonists are also gaining popularity as an antiplatelet agents during peri-PCI period. This review article summarizes the indications, duration of dual antiplatelet therapy (DAPT) in ACS setting. Antiplatelet therapy in special situations like atrial fibrillation and thrombocytopenia are discussed here. Issues like switching between P2Y12 inhibitors and genetic testing of antiplatelet agents are also mentioned in this article
University Heart Journal Vol. 17, No. 1, Jan 2021; 66-70
The Relationship of Brith Ball Therapy on Primigravida Mothers With A Fair Delivery Process
Authors: Novianti Novianti , Tridiyawati Feva 
Published: June 2022

Aims: Pain in childbirth has the highest degree of pain, caused many women are not ready to have children because day imagine the pain that wiil be presented at the time of labor later.
Objectives: The general objective of this study was to determine whether there was an effect of brith ball therapy on pain intensity in labour. Methods: Pain was measured using numeric pain scale from 0 to 10 (0 indicating no pain and 10 indicating severe pain). Univariate and bivariate analysis using Wilcoxon test analysis.
Results: Of the 50 respondent mothers who carried out the variable therapy pre-test british ball who were very painful and 29 respondents had pain (58%) and a minority had little pain, 21 people (42%). And on the post-test respondent the least pain was increased by 32 respondents (64%), While the minority of pain was quite severe as many as 18 respondents (36%). A 0.00<0.05. The result are expected to have an effect on british therapy on labor pain. The results of the Wilcoxon test analysis with a dgree of significance(a)=0.05 and a P value of 0.00 were obtained (a<0.05) which can be seen from the value which means that Ho is rejected and Ha is accepted. Conclusion: there is an effect british ball therapy on labor pain. Future studies should be conducted using bigger sample size to explore the true effect using experimental study.
Polyvinylpyrrolidone-Modified Taxifolin Liposomes Promote Liver Repair by Modulating Autophagy to Inhibit Activation of the TLR4/NF-κB Signaling Pathway
Authors: Ding Qiteng , Liu Wencong , Liu Xinglong , Ding Chuanbo , Zhao Yingchun , Dong Ling , Chen Huiying , Sun Shuwen , Zhang Yiwen , Zhang Jinping , Wu Ming 
Published: June 2022

Taxifolin (TAX) is a hepatoprotective flavanol compound, which is severely limited by poor solubility and low bioavailability. Liposomes (Lips) are used as well-recognized drug carrier systems that improve the water solubility and bioavailability of drugs, but are easily damaged by gastric juice after oral administration, resulting in the release of drugs in the gastric juice. Therefore, it is important to find materials that modify liposomes and avoid the destruction of the liposomal phospholipid bilayer structure by the gastrointestinal environment. Taxifolin liposomes (TAX-Lips) were modified by polyvinylpyrrolidone-k30 (PVP-TAX-Lips) and manufactured using a thin-film hydration technique. Particle size (109.27 ± 0.50 nm), zeta potential (−51.12 ± 3.79 mV), polydispersity coefficient (PDI) (0.189 ± 0.007), and EE (84.7 ± 0.2%) of PVP-TAX-Lips were studied. In addition, the results of in vitro release experiments indicated that the cumulative release rates of TAX-Lips and PVP-TAX-Lips were 89.73 ± 5.18% and 65.66 ± 4.86% in the simulated gastric fluid after 24 h, respectively, while the cumulative release rates were 68.20 ± 4.98% and 55.66 ± 3.92% in the simulated intestinal fluid after 24 h, respectively. Moreover, PVP-TAX-Lips were able to reverse lipopolysaccharide and D-galactosamine (LPS/D-GalN)-induced acute liver injury (ALI) by inducing autophagy to inhibit the expression levels of the TLR4/NF-κB signaling pathway and inflammatory factors, which suggested that PVP-TAX-Lips played an important role in the prevention of ALI and also provided a promising drug delivery system for the application of TAX.
Osmotic Processor for Enabling Sensitive and Rapid Biomarker Detection via Lateral Flow Assays
Authors: Chen Sheng-You , Wu Abe Y. , Lunde Ruby , Lai James J. 
Published: June 2022

Urine is an attractive biospecimen for in vitro diagnostics, and urine-based lateral flow assays are low-cost devices suitable for point-of-care testing, particularly in low-resource settings. However, some of the lateral flow assays exhibit limited diagnostic utility because the urinary biomarker concentration is significantly lower than the assay detection limit, which compromises the sensitivity. To address the challenge, we developed an osmotic processor that statically and spontaneously concentrated biomarkers. The specimen in the device interfaces with the aqueous polymer solution via a dialysis membrane. The polymer solution induces an osmotic pressure difference that extracts water from the specimen, while the membrane retains the biomarkers. The evaluation demonstrated that osmosis induced by various water-soluble polymers efficiently extracted water from the specimens, ca. 5–15 ml/h. The osmotic processor concentrated the specimens to improve the lateral flow assays’ detection limits for the model analytes—human chorionic gonadotropin and SARS-CoV-2 nucleocapsid protein. After the treatment via the osmotic processor, the lateral flow assays detected the corresponding biomarkers in the concentrated specimens. The test band intensities of the assays with the concentrated specimens were very similar to the reference assays with 100-fold concentrations. The mass spectrometry analysis estimated the SARS-CoV-2 nucleocapsid protein concentration increased ca. 200-fold after the osmosis. With its simplicity and flexibility, this device demonstrates a great potential to be utilized in conjunction with the existing lateral flow assays for enabling highly sensitive detection of dilute target analytes in urine.
Use of Customized 3D-Printed Titanium Augment With Tantalum Trabecular Cup for Large Acetabular Bone Defects in Revision Total Hip Arthroplasty: A Midterm Follow-Up Study
Authors: Kong Keyu , Zhao Chen , Chang Yongyun , Qiao Hua , Hu Yi , Li Huiwu , Zhang Jingwei 
Published: June 2022

Aims: In revision total hip arthroplasty (THA), large acetabular bone defects pose challenges for surgeons. Recently, wide application of trabecular tantalum, which has outstanding biocompatibility and mechanical properties, and the development of three-dimensional (3D) printing have led to the introduction of new schemes for acetabular reconstruction. However, few studies have focused on the treatment of bone defects with customized 3D-printed titanium augments combined with tantalum trabecular cup. Thus, we aimed to evaluate the effect of this therapy in patients who underwent revision THAs.Patients and Methods: We included 23 patients with Paprosky type III acetabular bone defects who underwent revision THA between January 2013 and June 2019. The preoperative hip rotation center and functional score were compared with those at 2–7 years (average 4.7 years) postoperatively to evaluate the midterm prognosis of our treatment choice.Results: Postoperatively, the rotation centres of all hips were comparable with those of the contralateral hips. Hip function improved with average Harris Hip Score improved from 33.5 (22.7–40.2) to 86.1 (73.5–95.6) and average Oxford Hip Score improved from 8.3 (0–14) to 38.8 (35–48) during follow-up. One dislocation, which occurred due to extreme hip flexion within 6 weeks, was treated with closed reduction, and no recurrent dislocation occurred. No nerve injury, infection, aseptic loosening, or osteolysis were observed and no re-revision was performed in any patient.Conclusion: Satisfactory midterm outcomes were obtained with 3D-printed titanium augment combined with tantalum cup for the treatment of acetabular defects in revision THA. Changes in the Harris Hip Score and Oxford Hip Score suggested a significant improvement in hip function.
A Microbiota-Dependent Response to Anticancer Treatment in an In Vitro Human Microbiota Model: A Pilot Study With Hydroxycarbamide and Daunorubicin
Authors: Hobson Claire Amaris , Vigué Lucile , Magnan Mélanie , Chassaing Benoit , Naimi Sabrine , Gachet Benoit , Claraz Pauline , Storme Thomas , Bonacorsi Stephane , Tenaillon Olivier , Birgy André 
Published: June 2022

BackgroundAnticancer drug efficacy is linked to the gut microbiota’s composition, and there is a dire need to better understand these interactions for personalized medicine.In vitromicrobiota models are promising tools for studies requiring controlled and repeatable conditions. We evaluated the impact of two anticancer drugs on human feces in the MiniBioReactor Array (MBRA)in vitromicrobiota system.MethodsThe MBRA is a single-stage continuous-flow culture model, hosted in an anaerobic chamber. We evaluated the effect of a 5-day treatment with hydroxycarbamide or daunorubicine on the fecal bacterial communities of two healthy donors. 16S microbiome profiling allowed analysis of microbial richness, diversity, and taxonomic changes.ResultsIn this host-free setting, anticancer drugs diversely affect gut microbiota composition. Daunorubicin was associated with significant changes in alpha- and beta-diversity as well as in the ratio of Firmicutes/Bacteroidetes in a donor-dependent manner. The impact of hydroxycarbamide on microbiota composition was not significant.ConclusionWe demonstrated, for the first time, the impact of anticancer drugs on human microbiota composition, in a donor- and molecule-dependent manner in anin vitrohuman microbiota model. We confirm the importance of personalized studies to better predict drug-associated-dysbiosisin vivo, linked to the host’s response to treatment.
The Inverse Correlation Between the Duration of Lifetime Occupational Radiation Exposure and the Prevalence of Atrial Arrhythmia
Authors: Thirumal Rithika , Vanchiere Catherine , Bhandari Ruchi , Jiwani Sania , Horswell Ronald , Chu San , Chamaria Surbhi , Katikaneni Pavan , Boerma Marjan , Gopinathannair Rakesh , Olshansky Brian , Bailey Steven , Dominic Paari 
Published: June 2022

ObjectiveAdvancements in fluoroscopy-assisted procedures have increased radiation exposure among cardiologists. Radiation has been linked to cardiovascular complications but its effect on cardiac rhythm, specifically, is underexplored.MethodsDemographic, social, occupational, and medical history information was collected from board-certified cardiologists via an electronic survey. Bivariate and multivariable logistic regression analyses were performed to assess the risk of atrial arrhythmias (AA).ResultsWe received 1,478 responses (8.8% response rate) from cardiologists, of whom 85.4% were male, and 66.1% were ≤65 years of age. Approximately 36% were interventional cardiologists and 16% were electrophysiologists. Cardiologists &gt; 50 years of age, with &gt; 10,000 hours (h) of radiation exposure, had a significantly lower prevalence of AA vs. those with ≤10,000 h (11.1% vs. 16.7%, p = 0.019). A multivariable logistic regression was performed and among cardiologists &gt; 50 years of age, exposure to &gt; 10,000 radiation hours was significantly associated with a lower likelihood of AA, after adjusting for age, sex, diabetes mellitus, hypertension, and obstructive sleep apnea (adjusted OR 0.57; 95% CI 0.38–0.85, p = 0.007). The traditional risk factors for AA (age, sex, hypertension, diabetes mellitus, and obstructive sleep apnea) correlated positively with AA in our data set. Cataracts, a well-established complication of radiation exposure, were more prevalent in those exposed to &gt; 10,000 h of radiation vs. those exposed to ≤10,000 h of radiation, validating the dependent (AA) and independent variables (radiation exposure), respectively.ConclusionAA prevalence may be inversely associated with radiation exposure in Cardiologists based on self-reported data on diagnosis and radiation hours. Large-scale prospective studies are needed to validate these findings.
An Integrated Approach to Characterize Temporal–Spatial Variations in PM2.5 Concentrations at the Ground Level and Its Implication on Health Impact Assessments
Authors: Ho Ming-Shing , Lin Ming-Yeng , Wang Jung-Der , Young Li-Hao , Hsu Hui-Tsung , Hwang Bing-Fang , Tsai Perng-Jy 
Published: June 2022

Although PM2.5 concentrations measured by the governmental air quality monitoring station (AQMS) have been widely used for conducting exposure assessments, it might be not able to reflect the residents’ exposures, especially for those associated with ground emissions. The present study was conducted in a city area for 1 year. A mobile monitoring station (MMS) was established to measure the PM2.5 concentrations at the ground level. A significant linear relationship (R2 = 0.53) was found between the MMS-measured concentrations and the corresponding concentrations obtained from the AQMS (15 m above the ground level), and the former was ∼ 1.11 times (95% CI: 1.08-1.15) in magnitude higher than that of the latter. To characterize the spatial variation of the area, the MMS-measured values were further classified into three different regions. A consistent trend was found in the present study for all collected data as industry region≒urban region &gt; harbor region. The aforementioned results clearly indicate that the residents’ ambient PM2.5 exposures do have spatial differences. Seven-year AQMS-measured concentrations (i.e., AQMS7-yr) were used to establish the long-term PM2.5 concentrations at the ground level (i.e., MMS7-yr) of the three different regions using the linear regression equations obtained from the MMS and AQMS. Health impact functions and local health data were used to quantify the PM2.5-attributable health burden for both AQMS7-yr and MMS7-yr, respectively. Results show that the former is ∼ 10.4% lower in magnitude than the latter in the estimated lung cancer death attributed fraction (AF). In particular, the decrease of unit PM2.5 (μg/m3) would lead to a 0.75 and 0.71% decrease in the estimated AF of lung cancer death for AQMS7-yr and MMS7-yr, respectively. As a result, directly using AQMS7-yr would lead to an underestimation of ∼ 1,000 lung cancer deaths annually in Taiwan in comparison with those using MMS7-yr. The aforementioned results clearly indicate the importance of characterizing ground-level exposures for assessing the health impact of residents, and the methodology developed by the present study would be helpful for solving the aforementioned problem.
Family-Level Bio-Indication Does not Detect the Impacts of Dams on Macroinvertebrate Communities in a Low-Diversity Tropical River
Authors: Dusabe Marie Claire , Neubauer Thomas A. , Muvundja Fabrice A. , Hyangya Béni L. , Albrecht Christian 
Published: June 2022

The Ruzizi River, the outlet of Lake Kivu in the Albertine Rift, flows into Lake Tanganyika and is important for hydropower generation and irrigation. The impacts of 2 dams in the Ruzizi River on macroinvertebrate community composition and diversity were surveyed every 3 months from December 2015 to October 2017. Macroinvertebrate samples were collected at sites upstream and downstream and additionally at two sites further downstream of the dams, in both comparatively pristine and highly disturbed areas. Several indices (Shannon-Wiener index, Simpson index, Pielou’s evenness, Rare Family Prevalence, and Average Score Per Taxa) were used to determine the alpha diversity and evenness of macroinvertebrates at the family level. Our results showed little to no immediate effect of the dams on macroinvertebrate diversity. Macroinvertebrate composition differed slightly below the dams compared to upstream. Communities near Dam II had slightly higher diversity compared to Dam I, probably because the vicinity to Lake Kivu has an immediate effect on diversity upstream of the first dam and likely because Dam II is 30 years younger than Dam I. This study suggests the importance of using species-level indices to better understand the ecological impacts of dams on macroinvertebrate diversity of tropical rivers with low species diversity.
Identification of Core Genes and Screening of Potential Targets in Intervertebral Disc Degeneration Using Integrated Bioinformatics Analysis
Authors: Li Jianjun , Yu Cheng , Ni Songjia , Duan Yang 
Published: June 2022

Background: Intervertebral disc degeneration (IDD), characterized by diverse pathological changes, causes low back pain (LBP). However, prophylactic and delaying treatments for IDD are limited. The aim of our study was to investigate the gene network and biomarkers of IDD and suggest potential therapeutic targets.Methods: Differentially expressed genes (DEGs) associated with IDD were identified by analyzing the mRNA, miRNA, and lncRNA expression profiles of IDD cases from the Gene Expression Omnibus (GEO). The protein–protein interaction (PPI) network, Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis as well as miRNA–lncRNA–mRNA networks were conducted. Moreover, we obtained 71 hub genes and performed a comprehensive analysis including GO, KEGG, gene set enrichment analysis (GSEA), gene set variation analysis (GSVA), Disease Ontology (DO), methylation analysis, receiver operating characteristic (ROC) curve analysis, immune infiltration analysis, and potential drug identification. We finally used qRT-PCR to verify 13 significant DEGs in normal and degenerative nucleus pulposus cells (NPCs).Results: We identified 305 DEGs closely related to IDD. The GO and KEGG analyses indicated that changes in IDD are significantly associated with enrichment of the inflammatory and immune response. GSEA analysis suggested that cell activation involved in the inflammatory immune response amide biosynthetic process was the key for the development of IDD. The GSVA suggested that DNA repair, oxidative phosphorylation, peroxisome, IL-6-JAK-STAT3 signaling, and apoptosis were crucial in the development of IDD. Among the 71 hub genes, the methylation levels of 11 genes were increased in IDD. A total of twenty genes showed a high functional similarity and diagnostic value in IDD. The result of the immune cell infiltration analysis indicated that seven genes were closely related to active natural killer cells. The most relevant targeted hub genes for potential drug or molecular compounds were MET and PIK3CD. Also, qRT-PCR results showed that ARHGAP27, C15orf39, DEPDC1, DHRSX, MGAM, SLC11A1, SMC4, and LINC00887 were significantly downregulated in degenerative NPCs; H19, LINC00685, mir-185-5p, and mir-4306 were upregulated in degenerative NPCs; and the expression level of mir-663a did not change significantly in normal and degenerative NPCs.Conclusion: Our findings may provide new insights into the functional characteristics and mechanism of IDD and aid the development of IDD therapeutics.
Effect of Normobaric Oxygen Inhalation Intervention on Microcirculatory Blood Flow and Fatigue Elimination of College Students After Exercise
Authors: Peng Yong , Meng Liang , Zhu Huan , Wan Li , Chen Fen 
Published: June 2022

Objective: To explore the effect of normobaric oxygen inhalation intervention on microcirculatory blood flow of college students after exercise and the impact of the elimination of exercise-induced fatigue, to provide a theoretical and methodological reference for the rapid elimination of fatigue of college students after endurance exercise.Methods: Forty-eight male non-sports majors of Hubei University for nationalities were randomly divided into the control group (n = 24) and intervention group (n = 24). The subjects in both groups completed the same exercise program twice (running 3,000 m on the treadmill at maximum speed). After running, the issues in the intervention group inhaled portable oxygen for 30 min, and the control group recovered naturally. Microcirculatory blood flow (MBP), blood flow velocity (AVBC), blood flow concentration (CMBC), muscle oxygen saturation (SmO2), heart rate (HR), blood lactic acid (BLA), blood urea (BU), and creatine kinase (CK) were measured before exercise, immediately after exercise and 30 min after exercise.Results: 1) MBP and AVBC had interaction between groups and time before and after exercise, MBP and AVBC immediately after exercise in the intervention group were significantly higher than those before exercise and 30 min after exercise, and 30 min after exercise in the intervention group were significantly higher than those in the control group. 2) SmO2, HR, BLA, BU, and CK had interaction between groups and time, and SmO2 immediately after exercise in the intervention group was significantly lower than that before exercise and 30 min after exercise, but significantly higher than that in the control group at 30 min after exercise. The HR and BLA immediately after exercise in the intervention group were significantly higher than those before exercise and 30 min after exercise, but significantly lower than those in the control group at 30 min after exercise, and the BU and CK in the intervention group were significantly higher than those before exercise, but significantly lower than those in the control group at 30 min after exercise.Conclusion: Normobaric oxygen inhalation for 30 min after exercise can delay the decrease of microcirculatory blood flow, increase muscle oxygen saturation, and promote the recovery of heart rate, blood lactic acid, blood urea and creatine kinase. Therefore, normobaric oxygen inhalation for 30 min after exercise can be used as an effective means to promote the elimination of exercise-induced fatigue after endurance running.