bluuebunny/crossref_metadata_2025_split huggingface dataset:
High prevalence of antinuclear antibodies in patients with chronic hepatitis C virus infection
Authors: de Castro Geison Luiz Costa , da Silva Graça Amoras Ednelza , Araújo Mauro Sérgio , da Silva Conde Simone Regina Souza , Bichara Carlos David Araújo , Queiroz Maria Alice Freitas , Vallinoto Antonio Carlos Rosário 
Published: September 2022

Abstract
Background
Hepatitis C virus (HCV) infection is a serious public health concern due to its high prevalence and mortality rate. In chronic infection, HCV may induce autoimmune responses through the production of autoantibodies, including antinuclear antibodies (ANA).

Methods
We assessed the presence of ANA by indirect immunofluorescence using HEp-2 cells in 89 patients with chronic hepatitis C. We also collected data on epidemiological variables; clinical characteristics; and biochemical, hematological, molecular, and histopathological information from the patients to assess the impact of the presence of ANA in those patients.

Results
The prevalence of ANA in the patients was 20.2%, which was significantly higher than that found in healthy controls (2%). However, there was no association of this marker with epidemiological, clinical-laboratory, molecular or histopathological characteristics of hepatitis C, although a slightly higher prevalence of ANA was detected in women and in patients infected with subgenotype 1a. In a specific analysis, chronic HCV patients with the “rods and rings” cytoplasmic pattern had higher degrees of hepatic fibrosis than did ANA-negative patients.

Conclusions
The results confirm a greater predisposition to the presence of ANA in patients with HCV, which may be associated with a worse prognosis, especially in the presence of the “rods and rings” cytoplasmic pattern.
Effect of explant type (leaf, stem) and 2,4-D concentration on callus induction: influence of elicitor type (biotic, abiotic), elicitor concentration and elicitation time on biomass growth rate and costunolide biosynthesis in gazania (Gazania rigens) cell suspension cultures
Authors: Mahood Huda E. , Sarropoulou Virginia , Tzatzani Thiresia-Teresa 
Published: September 2022

AbstractGazania rigens(L.) Gaertn. (Asteraceae) is a medicinal plant with high ornamental potential and use in landscaping. The therapeutic potential of sesquiterpene lactones (SLs) as plant natural products for pharmaceutical development has gained extensive interest with costunolide (chemical name: 6E,10E,11aR-6,10-dimethyl-3-methylidene-3a,4,5,8,9,11a-hexahydrocyclodeca[b]furan-2-one) used as a popular herbal remedy due to its anti-cancer, antioxidant, anti-inflammatory, anti-microbial, anti-allergic, and anti-diabetic activities, among others. In the present study, two explant types (leaf, stem) and four 2,4-dichlorophenoxy acetic acid (2,4-D) concentrations (0, 0.5, 1 and 2 mg/L) were tested for callusing potential. The results showed that stem explants treated with 1.5 mg/L 2,4-D exhibited higher callus induction percentage (90%) followed by leaf explants (80%) with 1 mg/L 2,4-D, after a 4-week period. Cell suspension cultures were established from friable callus obtained from stem explants following a sigmoid pattern of growth curve with a maximum fresh weight at 20 days of subculture and a minimum one at 5 days of subculture. In the following stage, the effects of elicitation of cell suspension cultures with either yeast extract (YE) or methyl jasmonate (MeJA), each applied in five concentrations (0, 100, 150, 200 and 250 mg/L) on cell growth (fresh and dry biomass) and costunolide accumulation were tested. After 20 days of culture, YE or MeJA suppressed cell growth as compared to the non-elicited cells, while costunolide accumulation was better enhanced under the effect of 150 mg/L MeJA followed by 200 mg/L YE, respectively. In the subsequent experiment conducted, the optimal concentration of the two elicitors (200 mg/L YE, 150 mg/L MeJA) was selected to investigate further elicitation time (0, 5, 10, 15 and 20 days). The results revealed that YE biotic elicitation stimulated cell growth and costunolide production, being maximum on day 20 for fresh biomass, on day 5 for dry biomass and on day 15 for the bioactive compound. Accordingly, cell growth parameters were maximized under the effect of abiotic elicitation with MeJA for 15 days, while highest costunolide content was achieved after 10 days. Overall, MeJA served as a better elicitor type than YE for biomass and costunolide production. Irrespective of elicitor type, elicitor concentration and elicitation time, maximal response was obtained with 150 mg/L MeJA for 10 days regarding costunolide accumulation (18.47 ppm) and 15 days for cell growth (fresh weight: 954 mg and dry weight: 76.3 mg). The application of elicitors can lead the large quantity of costunolide to encounter extensive range demand through marketable production without endangering ofG. rigens.Graphical Abstract
Flavonoid profile and antioxidant properties of Algerian common yew (Taxus baccata L.)
Authors: Bekhouche Mohamed , Benyammi Roukia , Slaoui Majda Khelifi , Krimat Soumia , Paris Cedric , Khelifi Lakhdar , Morsli Abdelkader 
Published: September 2022

Abstract
Background
In humans, various diseases are associated with the accumulation of free radicals. The antioxidants can scavenge free radicals and reduce their impact; thus, the search for effective natural antioxidants of plant origin is indispensable. The present study aims to determine, for the first time, the flavonoid compounds profile and to investigate the free radical scavenging and antioxidant properties of the methanolic extract of Taxus baccata L. from Algeria.

Methods
The determination of the flavonoid compound profile of the methanolic extract of Taxus baccata L. was established using high-performance liquid chromatography with diode-array detection coupled to electrospray ionization tandem mass spectrometry (HPLC–DAD–ESI–MS/MS). The total flavonoid content (TFC) was performed according to the aluminum chloride colorimetric method, while the free radical scavenging and antioxidant activities were carried out using three methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical assay, 2,2'-azino-bis3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical assay and ferric reducing antioxidant power (FRAP) Assay.

Results
A total of 26 compounds including flavon-3-ols, flavanonols, flavones, flavonols and bioflavonoids were characterized and identifiedusing HPLC–DAD–ESI–MS/MS analysis, five were reported for the first time such as taxifolin, apigenin, apigenin 7-O-glucoside, isorhamnetin 3-O-rutinoside and robustaflavone. The plant extract exhibited high total flavonoid content (TFC = 204.26 ± 6.02 mg RE/g dry extract) which corresponded to its strong radical scavenging activities [(DPPH IC50 = 35.31 ± 0.29 µg/ml and ABTS IC50 = 8.27 ± 0.52 µg/ml)] as compared to the synthetic antioxidant BHT [(DPPH IC50 = 78.96 ± 5.70 µg/ml and ABTS IC50 = 13.56 ± 0.06 µg/ml)]. However, the methanolic extract of T. baccata showed the lowest ferric reducing ability as compared to the positive controls (BHT, BHA, ascorbic acid, trolox and quercetin).

Conclusion
Our results imply that the Taxus Baccata L. might be a potential source for the isolation of natural antioxidant compounds.
An artificial neural network model based on autophagy-related genes in childhood systemic lupus erythematosus
Authors: Wu Jinting , Yang Wenxian , Li Huihui 
Published: September 2022

Abstract
Background
Childhood systemic lupus erythematosus (cSLE) is a multisystemic, life-threatening autoimmune disease. Compared to adults, SLE in childhood is more active, can cause multisystem involvement including renal, neurological and hematological, and can cause cumulative damage across systems more rapidly. Autophagy, one of the core functions of cells, is involved in almost every process of the immune response and has been shown to be associated with many autoimmune diseases, being a key factor in the interplay between innate and adaptive immunity. Autophagy influences the onset, progression and severity of SLE. This paper identifies new biomarkers for the diagnosis and treatment of childhood SLE based on an artificial neural network of autophagy-related genes.

Methods
We downloaded dataset GSE100163 from the Gene Expression Omnibus database and used Protein–protein Interaction Network (PPI) and Least Absolute Shrinkage and Selection Operator (LASSO) to screen the signature genes of autophagy-related genes in cSLE. A new artificial neural network model for cSLE diagnosis was constructed using the signature genes. The predictive efficiency of the model was also validated using the dataset GSE65391. Finally, "CIBERSORT" was used to calculate the infiltration of immune cells in cSLE and to analyze the relationship between the signature genes and the infiltration of immune cells.

Results
We identified 37 autophagy-related genes that differed in cSLE and normal samples, and finally obtained the seven most relevant signature genes for cSLE (DDIT3, GNB2L1, CTSD, HSPA8, ULK1, DNAJB1, CANX) by PPI and LASOO regression screening, and constructed an artificial neural network diagnostic model for cSLE. Using this model, we plotted the ROC curves for the training and validation group diagnoses with the area under the curve of 0.976 and 0.783, respectively. Finally, we performed immunoassays on cSLE samples, and the results showed that Plasma cells, Macrophages M0, Dendritic cells activated and Neutrophils were significantly infiltrated in cSLE.

Conclusion
We constructed an artificial neural network diagnostic model of seven autophagy-related genes that can be used for the diagnosis of cSLE. Meanwhile, the characteristic genes affect the immune infiltration of cSLE, which may provide new perspectives for the exploration of cSLE treatment and related mechanisms.
X-Linked Hypophosphatemia, Not Only a Skeletal Disease But Also a Chronic Inflammatory State
Authors: Méaux Marie-Noëlle , Alioli Candide , Linglart Agnès , Lemoine Sandrine , Vignot Emmanuelle , Bertholet-Thomas Aurélia , Peyruchaud Olivier , Flammier Sacha , Machuca-Gayet Irma , Bacchetta Justine 
Published: September 2022

Abstract

Context
X-linked hypophosphatemia (XLH) is a rare genetic disease caused by a primary excess of fibroblast growth factor 23 (FGF23). FGF23 has been associated with inflammation and impaired osteoclastogenesis, but these pathways have not been investigated in XLH.

Objective
This work aimed to evaluate whether XLH patients display peculiar inflammatory profile and increased osteoclastic activity.

Methods
We performed a prospective, multicenter, cross-sectional study analyzing transcript expression of 8 inflammatory markers (Il6, Il8, Il1β, CXCL1, CCL2, CXCR3, Il1R, Il6R) by real-time quantitative polymerase chain reaction on peripheral blood mononuclear cells (PBMCs) purified from total blood samples extracted from patients and healthy control individuals. The effect of native/active vitamin D on osteoclast formation was also assessed in vitro from XLH patients’ PBMCs.

Results
In total, 28 XLH patients (17 children, among them 6 undergoing standard of care [SOC] and 11 burosumab therapy) and 19 controls were enrolled. Expression of most inflammatory markers was significantly increased in PBMCs from XLH patients compared to controls. No differences were observed between the burosumab and SOC subgroups. Osteoclast formation was significantly impaired in XLH patients. XLH mature osteoclasts displayed higher levels of inflammatory markers, being however lower in cells derived from the burosumab subgroup (as opposed to SOC).

Conclusion
We describe for the first time a peculiar inflammatory profile in XLH. Since XLH patients have a propensity to develop arterial hypertension, obesity, and enthesopathies, and because inflammation can worsen these clinical outcomes, we hypothesize that inflammation may play a critical role in these extraskeletal complications of XLH.
The Strength of Parent–Adult Child Ties in Biological Families and Stepfamilies: Evidence From Time Diaries From Older Adults
Authors: Schoeni Robert F. , Freedman Vicki A. , Cornman Jennifer C. , Seltzer Judith A. 
Published: September 2022

Abstract
We examine older partnered parents' time spent with adult children in biological and step families, treating time together as an indication of relationship strength. Using a unique national sample of U.S. time diaries from the Panel Study of Income Dynamics, we investigate time with all adult children combined and with each adult child. We find that time together depends on family structure and parent–adult child dyadic relationship type embedded in family structure. In analyses of all adult children combined, an older parent is more likely to spend time with adult children in biological families than in stepfamilies only when there is no shared biological child in the stepfamily. In dyadic analyses, a parent's tie with an adult child who is a biological child of both partners is stronger in stepfamilies than in biological families. Moreover, among stepfamilies, ties are not uniformly stronger with biological children relative to stepchildren; differences emerge only in more complex families when each partner has biological children from previous relationships. Our findings challenge the view that ties with older parents are always weaker with stepchildren in stepfamilies and point to the importance of considering parent–child relationships in the broader family context.
The Postclassical Chronotope: A Narratological Inquiry
Authors: Lanser Susan S. , Rimmon-Kenan Shlomith 
Published: September 2022

Abstract
As a thought experiment, this article conjoins the approaches of two theorists with very different worldviews, Mikhail Bakhtin and Gérard Genette, in the hope of generating a model for a “postclassical chronotope” within the framework of a situated narratology. Our exploration begins with a juxtaposition of Virginia Woolf's Mrs Dalloway (1925), confined to one day but structured through the characters’ movements in space, with Amor Towles's A Gentleman in Moscow (2016), restricted to a single location but encompassing thirty-two years of time. To enrich the historical and ideological dimensions of the time-space relations, we extend our experiment to three other English-language novels that span two centuries: Jane Austen's Persuasion (1818), Zora Neale Hurston's Their Eyes Were Watching God (1937), and Mohsin Hamid's Exit West (2017). Although the authors’ choice of novels was almost arbitrary, the insights the exploration has yielded suggest that the “postclassical chronotope” warrants further research.
United We ’Gram: Scrolling through the Assimilated Aesthetics of Instapoetry
Authors: Knox JuEunhae 
Published: September 2022

Abstract
The phrase “Do it for the ’gram” has become extremely popular among the tech-savvy Generation Z, born in the 1990s and early 2000s and raised on social media. An escalation of the now long-established selfie, this colloquialism refers to the behavior of doing something specifically for the sake of posting a picture on Instagram. Publishing polished pictures for the sake of popularity does not merely apply to activities like brewing kombucha or practicing yoga, however, but in posting inspirational micro-poems. The extremely visual short-form genre of Instapoetry boasts 14.2 million posts to #poetsofinstagram alone, yet this so-called fidget-spinner literature has received little scholarly attention. While some magazines have featured the well-known Rupi Kaur, no academic work to date has extensively analyzed the aesthetics and cultural antecedents of Instapoetry. This article combines quantitatively collected data of common trends with in-depth analysis of case studies featured in Instagram's “Top 9” to show how the material and linguistic features of Instapoetry are in fact shaped and assimilated by their digital platform. By manually analyzing Instapoets’ practices in writing, stylization, and promotion, the author demonstrates that the sheer volume and rapidity of content production in turn encourages posts that are not only visually appealing but also immediately recognizable as Instapoems. Conforming to generic trends while simultaneously claiming genuineness and autonomy is a crucial method of increasing followers in a culture in which rapid swiping instantly consumes and buries masses of content. Examining the constant evolution of algorithmic “gatekeepers” and the pressures of the creative economy also contextualizes the challenges ingrained in creating “authentic” discourse on this heteronomous visual platform.
Political Partisanship, Trust, and Attitudes toward COVID-19 Vaccines in Indonesia
Authors: Halimatusa'diyah Iim , Durriyah Tati Lathipatud 
Published: September 2022

Abstract
Context: This study examines the extent to which political partisanship—measured as support for either the incumbent candidate for Indonesia's presidency, Joko Widodo (popularly known as Jokowi), or for Jokowi's challenger, Prabowo—affects individuals' risk perception of COVID-19 and COVID-19 vaccine hesitancy and refusal as well as beliefs about the safety and efficacy of the COVID-19 vaccine.
Methods: The authors performed multinomial logistic and ordinary least squares regression analyses on a nationally representative sample of a national survey on public trust in COVID-19 vaccines and vaccinations that was conducted in December 2020.
Findings: Individuals who voted for Prabowo in the 2019 presidential election were more likely to have a lower level of willingness and a higher level of hesitancy to get the COVID-19 vaccine than those who cast their ballot for Jokowi as the Indonesian president.
Conclusions: Political partisanship does matter in shaping individuals' hesitancy or refusal to receive the COVID-19 vaccine in Indonesia. The effect of partisanship is also significant in shaping individuals' trust in the efficacy and safety of the COVID-19 vaccine, but it is not significantly associated with individuals' risk perceptions.
PBAF chromatin remodeler complexes that mediate meiotic transitions in mouse
Authors: de Castro Rodrigo O. , Previato de Almeida Luciana , Carbajal Agustin , Gryniuk Irma , Pezza Roberto J. 
Published: September 2022

ABSTRACT
Gametogenesis in mammals encompasses highly regulated developmental transitions. These are associated with changes in transcription that cause characteristic patterns of gene expression observed during distinct stages of gamete development, which include specific activities with critical meiotic functions. SWI/SNF chromatin remodelers are recognized regulators of gene transcription and DNA repair, but their composition and functions in meiosis are poorly understood. We have generated gamete-specific conditional knockout mice for ARID2, a specific regulatory subunit of PBAF, and have compared its phenotype with BRG1 knockouts, the catalytic subunit of PBAF/BAF complexes. While Brg1Δ/Δ knockout acts at an early stage of meiosis and causes cell arrest at pachynema, ARID2 activity is apparently required at the end of prophase I. Striking defects in spindle assembly and chromosome-spindle attachment observed in Arid2Δ/Δ knockouts are attributed to an increase in aurora B kinase, a master regulator of chromosome segregation, at centromeres. Further genetic and biochemical analyses suggest the formation of a canonical PBAF and a BRG1-independent complex containing ARID2 and PBRM1 as core components. The data support a model in which different PBAF complexes regulate different stages of meiosis and gametogenesis.
Multimodal perioperative assessment of liver function and volume in patients undergoing hepatectomy for colorectal liver metastasis: a comparison of the indocyanine green retention test, 99mTc mebrofenin hepatobiliary scintigraphy and gadoxetic acid enhanced MRI
Authors: Wang Qiang , Brismar Torkel B. , Gilg Stefan , Jonas Eduard , Nilsson Henrik , Tzortzakakis Antonios , Isaksson Bengt , Axelsson Rimma , Sparrelid Ernesto 
Published: September 2022

Objectives: To compare the dynamic changes in future liver remnant (FLR) function and volume after hepatectomy and to evaluate the associations between three modalities in assessment of liver function. Methods: Liver function and volume were quantified pre-operatively, at post-operative day (POD) 7 and POD 28 in 10 patients with colorectal liver metastases undergoing hemihepatectomy using the indocyanine green retention (ICG) test, hepatobiliary scintigraphy (HBS) and gadoxetic acid-enhanced MRI. The 99mTc mebrofenin uptake rate in the FLR was applied as a reference of liver function. MRI-derived parameters including liver-to-muscle ratio (LMR), liver-to-spleen ratio (LSR) and hepatocellular uptake index (HUI) were used for liver function assessment. Spearman’s correlation analysis was used to evaluate the associations. Results: Increase in liver function ranged from 13 to 152% (median 92%) and in volume from 37 to 134% (median 79%). There was no significant discrepancy in increase between FLR function and volume during the first month following hepatectomy. LMR showed a significant correlation to ICG test (r = −0.66, p < 0.05) while LSR had an association with standardized FLR function obtained by HBS (r = −0.71, p < 0.05). During the first week after hepatectomy, pre-operative HUI and LMR showed the strongest correlation to the FLR growth in function and volume respectively (p < 0.05). Conclusion: The observed growth in FLR volume is closely related to the functional increase within 1 month after hepatectomy. Gadoxetic acid-enhanced MRI might substitute HBS for regional liver function assessment and provide an imaging tool for liver growth prediction. Advances in knowledge: Liver function growth was parallel with liver volume increase during the perioperative period. Liver function assessment with gadoxetic acid-enhanced MRI was comparable with that of HBS indicating that gadoxetic acid-enhanced MRI could substitute HBS for regional liver function evaluation.
Improving a trial participant information sheet through user testing in British South Asian women
Authors: Masood Yumna , Bower Peter , Knapp Peter , McMullen Sarah , Waheed Waquas 
Published: September 2022

Background: People from ethnic minorities in the UK have higher rates of mental health problems and are less likely to take part in mental health research. Previous research indicates that participant information sheets (PIS) are complex, and this complexity may impair comprehension more significantly for ethnic minority participants. Improving the readability of patient information can be achieved through user testing. We aimed to improve the readability of a PIS through user testing in an ethnic minority group in an ongoing trial (ROSHNI-2). Methods: An independent groups design was used to test the readability of the ROSHNI-2 PIS among South Asian women. There were two rounds of testing (n=31 women in total). South Asian women were asked to read the original PIS, then find and demonstrate an understanding of 19 key aspects of the trial. Based on the findings from the first round, the PIS was rewritten, redesigned, and retested. Results: Using the original PIS, only 2 of the 19 (11%) questions were found and understood by at least 80% of participants. In the revised PIS, 6 of the 19 (32%) questions were found and understood by at least 80% of participants. Conclusions: User testing and re-writing of a PIS resulted in a higher proportion of participants comprehending important information about a trial. This approach could improve participation in trials among ethnic minority communities.