bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome analysis of AAV-induced retinopathy models expressing human VEGF, TNF-α, and IL-6 in murine eyes
Authors: Becker Kolja , Weigelt Carina M. , Fuchs Holger , Viollet Coralie , Rust Werner , Wyatt Hannah , Huber Jochen , Lamla Thorsten , Fernandez-Albert Francesc , Simon Eric , Zippel Nina , Bakker Remko A. , Klein Holger , Redemann Norbert H. 
Published: November 2022

AbstractRetinopathies are multifactorial diseases with complex pathologies that eventually lead to vision loss. Animal models facilitate the understanding of the pathophysiology and identification of novel treatment options. However, each animal model reflects only specific disease aspects and understanding of the specific molecular changes in most disease models is limited. Here, we conducted transcriptome analysis of murine ocular tissue transduced with recombinant Adeno-associated viruses (AAVs) expressing either human VEGF-A, TNF-α, or IL-6. VEGF expression led to a distinct regulation of extracellular matrix (ECM)-associated genes. In contrast, both TNF-α and IL-6 led to more comparable gene expression changes in interleukin signaling, and the complement cascade, with TNF-α-induced changes being more pronounced. Furthermore, integration of single cell RNA-Sequencing data suggested an increase of endothelial cell-specific marker genes by VEGF, while TNF-α expression increased the expression T-cell markers. Both TNF-α and IL-6 expression led to an increase in macrophage markers. Finally, transcriptomic changes in AAV-VEGF treated mice largely overlapped with gene expression changes observed in the oxygen-induced retinopathy model, especially regarding ECM components and endothelial cell-specific gene expression. Altogether, our study represents a valuable investigation of gene expression changes induced by VEGF, TNF-α, and IL-6 and will aid researchers in selecting appropriate animal models for retinopathies based on their agreement with the human pathophysiology.
Comparative study for removal of phosphorus from aqueous solution by natural and activated bentonite
Authors: Elgarhy Abdelbaky Hossam , Mahran Belal N. A. , Liu Guanglong , Salem Talaat A. , ElSayed ElSayed ElBastamy , Ibrahim Lubna A. 
Published: November 2022

AbstractThe novelty of the current article is to investigate the adsorption potential of the Egyptian natural and activated bentonite (Na-bentonite) to inorganic and organic phosphorus (IP, OP) in aqueous media. The natural bentonite (NB) was activated to Na-bentonite (Na-B) by a new facile method within 2 h. NB and Na-B were also characterized using XRD, XRF, BET ESM, and FT-IR. The batch experiment has been employed to select the ideal conditions for the removal of inorganic and organic phosphorus (IP, OP) from aqueous solutions. The findings clearly showed that the Na-bentonite is enriched with sodium in the form of Na-montmorillonite with a higher specific area 138.51 m2/g than the value for the natural bentonite 74.21 m2/g. The batch experiment showed maximum absorption for both IP and OP adsorbents occurred at an equilibrium pH = 6, contact time of 40 to 50 min, 40 °C temperature, and a dose rate of 2 mg/L and 1 mg/L, respectively. The equilibrium data displayed better adjustment to Langmuir than the Freundlich, Temkin, and Dubinin-Radushkevich isotherms and adsorption kinetics followed the pseudo-second-order-type kinetic, and the parameters of thermodynamics reveal that adsorption occurs spontaneously and exothermic nature. Na-bentonite proved to be more efficient in removing target material than natural bentonite. The spent bentonites were easily regenerated by chemical methods.
Transport properties of two-dimensional dissipative flow of hybrid nanofluid with Joule heating and thermal radiation
Authors: Alqahtani Aisha M. , Ould Sidi Maawiya , Khan M. Riaz , Elkotb Mohamed Abdelghany , Tag-Eldin Elsayed , Galal Ahmed M. 
Published: November 2022

AbstractThe important feature of the current work is to consider the pressure variation, heat transport, and friction drag in the hydromagnetic radiative two-dimensional flow of a hybrid nanofluid depending on the viscous dissipation and Joule heating across a curved surface. The curved surface has been considered with the binary heating process called as prescribed heat flux and surface temperature. The basic partial differential equation (PDEs) has been converted into the non-dimensional ordinary differential equations (ODEs) by applying some specified dimensionless transformations. The bvp4c built-in package in MATLAB has been considered to find the numerical solution of the consequential equations. The graphical results have been plotted in terms of pressure, friction drag, velocity, temperature, and heat transport. Several important results have also been plotted for the plan level surface $$($$
(
The condition of $$K\to \infty )$$

K
→
∞
)

. It is found that the heat transport rate respectively reduces and enhances with the enhancement of radiation parameter and Hartmann number as well as the friction drag is enhancing with the high-volume fraction of nanoparticles and Hartmann number. Moreover, enhancing curvature parameter, enhances the friction drag and declines the heat transport rate. The current work renders uncountable applications in several engineering and industrial systems like electronic bulbs, electric ovens, geysers, soil pollution, electric kettle, fibrous insulation, etc. Moreover, the heating as well as the cooling systems of electrical, digital, and industrial instruments, are controlled by the heat transport in fluids. Thus, it is important to use such flows in these types of instruments.
Simple one-step synthesis of urchin-like Fe–Mn nanostructures via statistical design and their effects on the morphology
Authors: Naeinimohammadi Saeid , Rahbar-Kelishami Ahmad 
Published: November 2022

AbstractIn the current investigation, a new urchin-like nanostructure using an authorized one-pot precipitation technique was synthesized using Taguchi statistical design. The effect of factors, including the concentration of FeSO4⋅7H2O, KMnO4, NaOH, and reaction temperature, on the diameter-to-length ratio of the nanoneedles and the regularity or irregularity morphology of other samples, was investigated. KMnO4 and FeSO4⋅7H2O, with a contribution of 32.62% and 30.9%, had the most substantial effect on the nanoneedles' diameter. The surface morphology and chemical composition of the as-prepared samples were studied using field emission scanning electron microscopy, X-ray diffraction, and energy dispersive spectroscopy (EDX) analyses. The length and needle diameter was obtained 140 and 17 nm, respectively. The result of BET analysis for the highest and the lowest value of D/L shows that the smallest ratio of diameter to length has a high specific surface area. The results show that sample S4 has a particular surface area of 74 m2/g, much more than S3 (25 m2/g). The estimated crystallite size in synthesized S3 and S4 samples are 41.64 and 26.49 nm, respectively.
Investigation of Ti/Al2O3 + TiO2 and Ti + TiO2/Al2O3 + TiO2 hybrid coatings as protection of ultra-light Mg–(Li)–Al–RE alloys against corrosion
Authors: Staszuk Marcin , Pakuła Daniel , Reimann Łukasz , Musztyfaga-Staszuk Małgorzata , Socha Robert , Tański Tomasz 
Published: November 2022

AbstractLow corrosion resistance is a significant problem of magnesium alloys, particularly ultra-light magnesium-lithium alloys. Surface treatment is one way to improve their corrosion resistance. The paper presents the results of tests of Ti/Al2O3 + TiO2 and Ti + TiO2/Al2O3 + TiO2 coatings obtained in a hybrid process combining PVD and ALD methods and ALD coating of Al2O3 + TiO2 type obtained on AE42 (Mg–4Li–2RE) and LAE442 (Mg–4Li–4Al–2RE). Structural studies were performed using scanning and transmission electron microscopy (SEM and TEM), atomic force microscopy and EDS and XPS spectroscopic methods. Potentiodynamic tests and electrochemical impedance spectroscopy EIS in 0.05 M NaCl solution were performed to determine the electrochemical properties of the tested materials. Moreover, tests of surface wettability and tribological properties using the ball-on-disc method were performed. Based on the analysis of anodic polarisation curves and Tafel analysis, it was found that the Ti + TiO2/Al2O3 + TiO2 coating showed the best potentiodynamic properties on both substrates. In particular, on the magnesium-lithium substrate, the value of the polarisation resistance of this hybrid coating is Rpol = 14 × 103 Ω × cm2, and the value of the corrosion current is jcorr = 0.4 µA/cm2. For the uncoated LAE442 substrate, the polarisation resistance is Rpol = 1.05 × 103 Ω × cm2, and the corrosion current value is jcorr = 5.49 µA/cm2. This improvement is due to the synergistic effect of the combined PVD and ALD technologies. The study confirmed the impact of hybrid coatings on improving the anti-corrosion and tribological properties of ultra-light magnesium alloys.
Anti-counterfeiting system based on luminescent varnish enriched by NIR- excited nanoparticles for paper security
Authors: Przybylska D. , Grzyb T. , Erdman A. , Olejnik K. , Szczeszak A. 
Published: November 2022

AbstractUp-converting nanoparticles can be a demand for requirements in many areas, including bioimaging and conversion of energy, but also in the battle against counterfeiting. The properties of lanthanide ions make falsification difficult or even impossible using appropriately designed systems. The proposition of such an approach is the NaErF4:Tm3+@NaYF4 core@shell up-converting nanoparticles combined with transparent varnishes. Given the spectroscopic properties of Er3+ ions present in the fluoride matrix, the obtained up-converting nanoparticles absorb light by 808 and 975 nm wavelengths. The intentionally co-doped Tm3+ ions enable tuning characteristic green Er3+ emission to red luminescence, particularly desirable in anti-counterfeiting applications. The article includes a thorough analysis of structural and morphological properties. Moreover, this work shows that exclusive luminescent properties of NaErF4:Tm3+@NaYF4 NPs can be given to the transparent varnish, providing an excellent anti-counterfeiting system, revealing red emission under two different excitation wavelengths.
RAB27A promotes the proliferation and invasion of colorectal cancer cells
Authors: Li Qingyan , Zhao Huixia , Dong Weiwei , Guan Na , Hu Yanyan , Zeng Zhiyan , Zhang He , Zhang Fengyun , Li Qiuwen , Yang Jingwen , Xiao Wenhua 
Published: November 2022

AbstractColorectal cancer (CRC) is one of the most commonly diagnosed cancer types worldwide. Despite significant advances in prevention and diagnosis, CRC is still one of the leading causes of cancer-related mortality globally. RAB27A, the member of RAB27 family of small GTPases, is the critical protein for intracellular secretion and has been reported to promote tumor progression. However, it is controversial for the role of RAB27A in CRC progression, so we explored the exact function of RAB27A in CRC development in this study. Based on the stable colon cancer cell lines of RAB27A knockdown and ectopic expression, we found that RAB27A knockdown inhibited proliferation and clone formation of SW480 colon cancer cells, whereas ectopic expression of RAB27A in RKO colon cancer cells facilitated cell proliferation and clone formation, indicating that RAB27A is critical for colon cancer cell growth. In addition, our data demonstrated that the migration and invasion of colon cancer cells were suppressed by RAB27A knockdown, but promoted by RAB27A ectopic expression. Therefore, RAB27A is identified as an onco-protein in mediating CRC development, which may be a valuable prognostic indicator and potential therapeutic target for CRC.
Diversity of gammacoronaviruses and deltacoronaviruses in wild birds and poultry in Russia
Authors: Marchenko Vasily , Danilenko Alexey , Kolosova Natalia , Bragina Maria , Molchanova Marina , Bulanovich Yuliya , Gorodov Vladimir , Leonov Sergey , Gudymo Andrey , Onkhonova Galina , Svyatchenko Svetlana , Ryzhikov Alexander 
Published: November 2022

AbstractCoronaviruses of the genera Gammacoronavirus and Deltacoronavirus are globally widespread and circulate primarily in wild and domestic birds. Prior studies have established frequently occurring crossover events from avian to mammalian reservoirs. However, there is limited understanding of the diversity and geographical distribution of coronaviruses among birds. In this study, the surveillance of coronaviruses in birds in Russia during 2020 revealed the presence of coronaviruses in 12% of samples from birds. Targeted NGS approach was used for the evaluation of genetic diversity based on RdRp gene. While gammacoronviruses were found in both wild birds and poultry, deltacoronaviruses were found in wild birds only and represent the first detections for Russia. A number of cases with the simultaneous detection of gamma- and deltacoronaviruses in one bird was reported. The results of this study highlight the importance of further research concerning the spread and diversity of coronaviruses among birds within and migrating throughout the territory of Russia across the globe.
Structure-guided insights into potential function of novel genetic variants in the malaria vaccine candidate PfRh5
Authors: Mangou Khadidiatou , Moore Adam J. , Thiam Laty Gaye , Ba Aboubacar , Orfanó Alessandra , Desamours Ife , Ndegwa Duncan Ndungu , Goodwin Justin , Guo Yicheng , Sheng Zizhang , Patel Saurabh D. , Diallo Fatoumata , Sene Seynabou D. , Pouye Mariama N. , Faye Awa Thioub , Thiam Alassane , Nunez Vanessa , Diagne Cheikh Tidiane , Sadio Bacary Djilocalisse , Shapiro Lawrence , Faye Ousmane , Mbengue Alassane , Bei Amy K. 
Published: November 2022

AbstractThe recent stall in the global reduction of malaria deaths has made the development of a highly effective vaccine essential. A major challenge to developing an efficacious vaccine is the extensive diversity of Plasmodium falciparum antigens. While genetic diversity plays a major role in immune evasion and is a barrier to the development of both natural and vaccine-induced protective immunity, it has been under-prioritized in the evaluation of malaria vaccine candidates. This study uses genomic approaches to evaluate genetic diversity in next generation malaria vaccine candidate PfRh5. We used targeted deep amplicon sequencing to identify non-synonymous Single Nucleotide Polymorphisms (SNPs) in PfRh5 (Reticulocyte-Binding Protein Homologue 5) in 189 P. falciparum positive samples from Southern Senegal and identified 74 novel SNPs. We evaluated the population prevalence of these SNPs as well as the frequency in individual samples and found that only a single SNP, C203Y, was present at every site. Many SNPs were unique to the individual sampled, with over 90% of SNPs being found in just one infected individual. In addition to population prevalence, we assessed individual level SNP frequencies which revealed that some SNPs were dominant (frequency of greater than 25% in a polygenomic sample) whereas most were rare, present at 2% or less of total reads mapped to the reference at the given position. Structural modeling uncovered 3 novel SNPs occurring under epitopes bound by inhibitory monoclonal antibodies, potentially impacting immune evasion, while other SNPs were predicted to impact PfRh5 structure or interactions with the receptor or binding partners. Our data demonstrate that PfRh5 exhibits greater genetic diversity than previously described, with the caveat that most of the uncovered SNPs are at a low overall frequency in the individual and prevalence in the population. The structural studies reveal that novel SNPs could have functional implications on PfRh5 receptor binding, complex formation, or immune evasion, supporting continued efforts to validate PfRh5 as an effective malaria vaccine target and development of a PfRh5 vaccine.
Development of truncated elastin-like peptide analogues with improved temperature-response and self-assembling properties
Authors: Sumiyoshi Shogo , Suyama Keitaro , Tanaka Naoki , Andoh Takumi , Nagata Akihiko , Tomohara Keisuke , Taniguchi Suguru , Maeda Iori , Nose Takeru 
Published: November 2022

AbstractFunctional peptides, which are composed of proteinogenic natural amino acids, are expected to be used as biomaterials with minimal environmental impact. Synthesizing a functional peptide with a shorter amino acid sequence while retaining its function is a easy and economical strategy. Furthermore, shortening functional peptides helps to elucidate the mechanism of their functional core region. Truncated elastin-like peptides (ELPs) are peptides consisting of repetitive sequences, derived from the elastic protein tropoelastin, that show the thermosensitive formation of coacervates. In this study, to obtain shortened ELP analogues, we synthesized several (Phe-Pro-Gly-Val-Gly)n (FPGVG)n analogues with one or two amino acid residues deleted from each repeat sequence, such as the peptide analogues consisting of FPGV and/or FPG sequences. Among the novel truncated ELP analogues, the 16-mer (FPGV)4 exhibited a stronger coacervation ability than the 25-mer (FPGVG)5. These results indicated that the coacervation ability of truncated ELPs was affected by the amino acid sequence and not by the peptide chain length. Based on this finding, we prepared Cd2+-binding sequence-conjugated ELP analogue, AADAAC-(FPGV)4, and found that it could capture Cd2+. These results indicated that the 16-mer (FPGV)4 only composed of proteinogenic amino acids could be a new biomaterial with low environmental impact.
Use of probabilistic fastener velcro as a friction-induced vibration damping treatment
Authors: Kwon Semin , Jeon Jonghoon , Yoo Seungjeok , Park Junhong 
Published: November 2022

AbstractProbabilistic fasteners are biologically inspired clamping devices that are interlocked by stems on each surface. Due to dynamic characteristics of fastening mechanism, friction inevitably occurs between stems in a vibrating environment. The use of the probabilistic fastener as a vibration reduction component were investigated with advantages from friction-induced damping in this study. The dynamic stiffness and loss factor of the probabilistic fastener were derived from the vibration interaction with a mechanical structure. This allowed determination of energy dissipation due to the friction in hook and loop from the wave propagation analysis. As the vibration amplitude increased, the loss factor of the fastener gradually increased because the friction between multiple stems increased. With the probabilistic fastener application, the vibration generation and transmission were reduced compared to the bolted joint due to the inherent frictional contacts. With this unique advantage, the probabilistic fastener has potential applications when large damping is required with additional benefit on the reduced weight.
Winding loss analysis of planar spiral coil and its structure optimization technique in wireless power transfer system
Authors: Chen Qingbin , Zhang Xu , Chen Wei , Wang Cong 
Published: November 2022

AbstractCircular planar spiral coils are wildly used for the magnetic coupling system in a high-frequency wireless power transfer system. The loss of the magnetic coupling system usually takes dominance in the whole system. This paper built the calculation model of magnetic field strength and coil loss, the proposed calculation model can effectively consider the mutual influence between the transmitter and receiver coil and accurately calculated the AC loss of WPT coils. Then, the effect of turn spacing on the AC resistance of coil is analyzed. It reveals that the proximity effect loss is greater when the coil is tightly wound, and the AC loss can be optimized by designing the turn spacing. Based on the above analysis, a double-layer coil method is proposed. This method can reduce the AC loss and improve the quality factor (Q) without changing the mutual inductance and footprint of coil at high frequency. The AC resistance of the double-layer coil method can be greatly reduced compared with the general method through simulation and experiment. The work efficiency of WPT system is increased by 4.3%, which verifies the accuracy and flexibility of theoretical analysis.
Obesity downregulates lipid metabolism genes in first trimester placenta
Authors: Rasool Aisha , Mahmoud Taysir , Mathyk Begum , Kaneko-Tarui Tomoko , Roncari Danielle , White Katharine O. , O’Tierney-Ginn Perrie 
Published: November 2022

AbstractPlacentas of obese women have low mitochondrial β-oxidation of fatty acids (FA) and accumulate lipids in late pregnancy. This creates a lipotoxic environment, impairing placental efficiency. We hypothesized that placental FA metabolism is impaired in women with obesity from early pregnancy. We assessed expression of key regulators of FA metabolism in first trimester placentas of lean and obese women. Maternal fasting triglyceride and insulin levels were measured in plasma collected at the time of procedure. Expression of genes associated with FA oxidation (FAO; ACOX1, CPT2, AMPKα), FA uptake (LPL, LIPG, MFSD2A), FA synthesis (ACACA) and storage (PLIN2) were significantly reduced in placentas of obese compared to lean women. This effect was exacerbated in placentas of male fetuses. Placental ACOX1 protein was higher in women with obesity and correlated with maternal circulating triglycerides. The PPARα pathway was enriched for placental genes impacted by obesity, and PPARα antagonism significantly reduced 3H-palmitate oxidation in 1st trimester placental explants. These results demonstrate that obesity and hyperlipidemia impact placental FA metabolism as early as 7 weeks of pregnancy.
Electronic fluctuation difference between trimethylamine N-oxide and tert-butyl alcohol in water
Authors: Kuroki Nahoko , Uchino Yukina , Funakura Tamon , Mori Hirotoshi 
Published: November 2022

AbstractAlthough small organic molecules in cells have been considered important to control the functions of proteins, their electronic fluctuation and the intermolecular interaction, which is physicochemical origin of the molecular functions, under physiological conditions, i.e., dilute aqueous solutions (0.18 mol L−1), has never been clarified due to the lack of observation methods with both accuracy and efficiency. Herein, the time evolutions of the interactions in dilute aqueous trimethylamine N-oxide (TMAO) and tert-butyl alcohol (TBA) solutions were analyzed via ab initio molecular dynamics simulations accelerated with the fragment molecular theory. It has been known that TMAO and TBA have similar structures, but opposite physiological functions to stabilize and destabilize proteins. It was clarified that TMAO induced stable polarization and charge-transfer interactions with water molecules near the hydrophilic group, and water molecules were caught even near the CH3– group. Those should affect protein stabilization. Understanding the solution dynamics will contribute to artificial chaperone design in next generation medicine.