bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined transcriptome and proteome analysis of yak PASMCs under hypoxic and normoxic conditions
Authors: Zhang Lan , Zhang Yiyang , Zhou Juan , Yao Yifan , Li Rui , Zhou Manlin , Chen Shuwu , Qiao Zilin , Yang Kun 
Published: November 2022

Background
Yaks are animals that have lived in plateau environments for generations. Yaks can adapt to the hypoxic plateau environment and also pass this adaptability on to the next generation. The lungs are the most important respiratory organs for mammals to adapt to their environment. Pulmonary artery smooth muscle cells play an important role in vascular remodeling under hypoxia, but the genetic mechanism underpinning the yak’s ability to adapt to challenging plateau conditions is still unknown.

Methods
A tandem mass tag (TMT) proteomics study together with an RNA-seq transcriptome analysis were carried out on pulmonary artery smooth muscle cells (PASMCs) that had been grown for 72 hours in both normoxic (20% O2) and hypoxic (1% O2) environments. RNA and TP (total protein) were collected from the hypoxic and normoxic groups for RNA-seq transcriptome sequencing and TMT marker protein quantification, and RT-qPCR validation was performed.

Results
A total of 17,711 genes and 6,859 proteins were identified. Further, 5,969 differentially expressed genes (DEGs) and 531 differentially expressed proteins (DEPs) were identified in the comparison group, including 2,924 and 186 upregulated genes and proteins and 3,045 and 345 down-regulated genes and proteins, respectively. The transcriptomic and proteomic analyses revealed that 109 DEGs and DEPs were highly positively correlated, with 77 genes showing the same expression trend. Nine overlapping genes were identified in the HIF-1 signaling pathway, glycolysis / gluconeogenesis, central carbon metabolism in cancer, PPAR signaling pathway, AMPK signaling pathway, and cholesterol metabolism (PGAM1, PGK1, TPI1, HMOX1, IGF1R, OLR1, SCD, FABP4 and LDLR), suggesting that these differentially expressed genes and protein functional classifications are related to the hypoxia-adaptive pathways. Overall, our study offers abundant data for further analysis of the molecular mechanisms in yak PASMCs and their adaptability to different oxygen concentrations.
Recovery of sweet taste preference in adult rats following bilateral chorda tympani nerve transection
Authors: Padalhin Andrew , Abueva Celine , Park So Young , Ryu Hyun Seok , Lee Hayoung , Kim Jae Il , Chung Phil-Sang , Woo Seung Hoon 
Published: November 2022

Background
Numerous studies have noted the effect of chorda tympani (CT) nerve transection on taste sensitivity yet very few have directly observed its effects on taste receptor and taste signaling protein expressions in the tongue tissue.

Methods
In this study, bilateral CT nerve transection was performed in adult Sprague Dawley rats after establishing behavioral taste preference for sweet, bitter, and salty taste via short term two-bottle preference testing using a lickometer setup. Taste preference for all animals were subsequently monitored. The behavioral testing was paired with tissue sampling and protein expression analysis. Paired groups of CT nerve transected animals (CTX) and sham operated animals (SHAM) were sacrificed 7, 14, and 28 days post operation.

Results
Immunofluorescence staining of extracted tongue tissues shows that CT nerve transection resulted in micro-anatomical changes akin to previous investigations. Among the three taste qualities tested, only the preference for sweet taste was drastically affected. Subsequent results of the short-term two-bottle preference test indicated recovery of sweet taste preference over the course of 28 days. This recovery could possibly be due to maintenance of T1R3, GNAT3, and TRPM5 proteins allowing adaptable recovery of sweet taste preference despite down-regulation of both T1R2 and Sonic hedgehog proteins in CTX animals. This study is the first known attempt to correlate the disruption in taste preference with the altered expression of taste receptors and taste signaling proteins in the tongue brought about by CT nerve transection.
Panorama econômico da produção e exportação de mel de abelha produzidos no Brasil
Authors: Trevisol Graciela , Pinheiro Bueno Miriam , Leonardo de Oliveira João Paulo , Macedo Karla Gonçalves 
Published: November 2022

O mel, a geleia real e as própolis são produtos provenientes da apicultura. Essas são mercadorias importantes que o Brasil exporta e possui um mercado promissor no exterior. O mel destaca-se como uma fonte de renda alternativa, pela facilidade de ser desenvolvida em todas as regiões do país, por causa de sua flora diversificada e clima favoráveis, o que contribui para a sua produção durante o ano todo. Este artigo, por meio de pesquisas bibliográficas, teve por objetivo analisar economicamente a produção e a exportação desse produto; considerando o panorama econômico de mel de abelha no país, no período de 2010 - 2020 (dez anos). Os resultados apontam que o Brasil conseguiu se destacar, nos últimos anos, como um dos principais produtores em nível mundial, porém, percebe-se que é possível que o país melhorar ainda mais sua posição no ranking quando aprimorar ainda mais a qualidade na profissionalização do produtor. Portanto, conclui-se que a apicultura brasileira tem potencial para aumentar a sua produção tanto de maneira quantitativa quanto qualitativa, por meio do aperfeiçoamento das técnicas de manejo e adoção de tecnologias adequadas ao seu contexto.
Effect of Explant Age and Applied IBA on Growth and Rooting of Apical Stem Cuttings of Potato for Early Generation Seed Potato Production
Authors: Gul Zishan 
Published: November 2022

The aim of this work is to study the effect of explant age and exogenously applied IBA on the growth and rooting potential of potato apical stem cuttings for production of first- generation seed potatoes. The experimental protocol consists in taking apical stem cuttings from potato plantlets at different physiological ages (30 and 60 days old) in interaction with the growth regulator indole-3-butyric acid (IBA) at different concentrations (100, 200, and 300 ppm) to study the production of potato minitubers. The results of the present study showed that mini-cuttings from young potato plants (30 days old) have a higher rooting capacity than mini-cuttings from mature plants. In addition, IBA concentrations, especially at 300 ppm, have a significant effect on most of the characteristics studied, e.g. rooting %, root number, root length, shoot length, and number of leaves. Regarding yield attributes, application of IBA at 300ppm gave the highest average tuber number (6.66) and tuber yield (142.97g) was higher in 30days old explant cuttings. The data presented in this study may encourage potato growers to use potato stem cuttings as a new, cheaper method of growing potatoes instead of expensive imported seed tubers. It is suggested that the use of apical stem cuttings can be a quick and effective method for mass propagation and to increase the yield of minitubers in the seed potato production cycle.
Can pasture‐fed livestock farming practices improve the ecological condition of grassland in Great Britain?
Authors: Norton Lisa R. , Maskell Lindsay C. , Wagner Markus , Wood Claire M. , Pinder Adam P. , Brentegani Michele 
Published: November 2022

Abstract
Livestock farming in Great Britain (GB) faces multiple pressures. Yet, grassland managed for livestock is the most extensive habitat in GB and is key to cultural landscapes and their biodiversity and soil health.
This study analysed a nationally representative dataset of over 940 large (200 m2) Neutral (agriculturally semi‐improved) and (agriculturally) Improved Grassland plots from the GB Countryside Survey (CS) to assess relationships between key grassland sward and soil variables. Analysis also looked at how these variables changed over time as plots switched between these grassland types. Data from grassland plots managed by Pasture‐Fed Livestock Association (PFLA) farmers were compared to CS plot data to assess the impacts of their practices on these variables.
Plant species richness in Neutral grassland types in CS plots was positively associated with total soil invertebrate abundance (total taxa) and soil N and C and negatively associated with soil P. There were negative relationships between the covers of Lolium sp. (Neutral only), legumes and forbs and soil C and moisture variables.
Grassland swards on PFLA member farms were characteristic of Neutral grassland. PFLA plots were more species rich and contained more legume and forb species and lower proportions of Lolium perenne than those on Improved Grassland. Vegetation height was greater in PFLA plots than in CS plots of either Improved or Neutral Grassland. Unlike CS Neutral Grassland plots, soil properties in PFLA plots were not significantly different from those for Improved Grassland for any measured variable (soil carbon concentration [C], bulk density, pH, nitrogen [N], phosphorus [P]).
Higher species richness in grasslands is associated with positive measures of soil health. PFLA plant communities contain relatively high species richness and tall vegetation, which is positive for biodiversity. Lack of positive measures of soil health associated with higher species richness recorded in PFLA grassland (as opposed to CS grassland) may reflect time lags in soil responses to management, as evidenced through an analysis of the impacts of land‐use change over time on CS plot characteristics. Our findings indicate that pasture‐fed livestock approaches may be beneficial for grassland and wider ecosystems.
Unknown piecewise constant parameters identification with exponential rate of convergence
Authors: Glushchenko Anton , Lastochkin Konstantin 
Published: November 2022

SummaryThe scope of this research is the identification of unknown piecewise constant parameters of linear regression equation under the finite excitation condition. Compared to the known methods, to make the computational burden lower, only one model to identify all switching states of the regression is used in the developed procedure with the following two‐fold contribution. First of all, we propose a new truly online estimation algorithm based on a well‐known DREM approach to detect switching time and preserve time alertness with adjustable detection delay. Second, despite the fact that a switching signal function is unknown, the adaptive law is derived that provides global exponential convergence of the regression parameters estimates to their true values in case the regressor is finitely exciting somewhere inside the time interval between two consecutive parameters switches. The robustness of the proposed identification procedure to the influence of external disturbances is analytically proved. Its effectiveness is demonstrated via numerical experiments, in which both abstract regressions and a second‐order plant model are used.
Bioactive MXene Promoting Angiogenesis and Skeletal Muscle Regeneration through Regulating M2 Polarization and Oxidation Stress
Authors: Li Ting , Ma Junping , Wang Wensi , Lei Bo 
Published: November 2022

AbstractComplete repair of skeletal muscles caused by severe mechanical damage and muscle‐related diseases remains a challenge. 2D Ti3C2Tx (MXene) possesses special photoelectromagnetic properties and has attracted considerable attention in materials science and engineering. However, the bioactive properties and potential mechanism of MXene in tissue engineering, especially in skeletal muscle regeneration, are unclear. Herein, the antioxidation and anti‐inflammation activities of MXene and its effects on myogenic differentiation and regeneration of skeletal muscle in vivo are investigated. In vitro studies have shown that MXene has excellent antioxidation and anti‐inflammatory properties, and promotes myogenic differentiation and angiogenesis. MXene can remove excess reactive oxygen species in macrophage cells to alleviate oxidative stress and induce the transformation of M1 macrophages into M2 macrophages to reduce excessive inflammation, which can significantly promote the proliferation and differentiation of myoblasts, as well as the proliferation, migration, and tube formation of endothelial cells. Animal experiments with rat tibial anterior muscle defects show that MXene can promote angiogenesis, muscle fiber formation, and skeletal muscle regeneration by regulating the cell microenvironment through anti‐inflammatory and antioxidant pathways. The findings suggest that MXene can be used as a multifunctional bioactive material to enhance tissue regeneration through robust antioxidation, anti‐inflammation, and angiogenesis activities.
Low‐Coordinated Pd Site within Amorphous Palladium Selenide for Active, Selective, and Stable H2O2 Electrosynthesis
Authors: Yu Zhiyong , Lv Shengyao , Yao Qing , Fang Nan , Xu Yong , Shao Qi , Pao Chih‐Wen , Lee Jyh‐Fu , Li Guoliang , Yang Li‐Ming , Huang Xiaoqing 
Published: November 2022

AbstractThe development of high‐performance catalysts with high activity, selectivity, and stability are essential for the practical applications of H2O2 electrosynthesis technology, but it is still formidably challenging. It is reported that the low‐coordinated structure of Pd sites in amorphous PdSe2 nanoparticles (a‐PdSe2 NPs) can significantly boost the electrocatalytic synthesis of H2O2. Detailed investigations and theoretical calculations reveal that the disordered arrangement of Pd atoms in a‐PdSe2 NPs can promote the activity, while the Pd sites with low‐coordinated environment can optimize the adsorption toward oxygenated intermediate and suppress the cleavage of O–O bond, leading to a significant enhancement in both the H2O2 selectivity and productivity. Impressively, a‐PdSe2 NPs/C exhibits high H2O2 selectivity over 90% in different pH electrolytes. H2O2 productivities with ≈3245.7, 1725.5, and 2242.1 mmol gPd−1 h−1 in 0.1 m KOH, 0.1 m HClO4, and 0.1 m Na2SO4 can be achieved, respectively, in an H‐cell electrolyzer, being a pH‐universal catalyst for H2O2 electrochemical synthesis. Furthermore, the produced H2O2 can reach 1081.8 ppm in a three‐phase flow cell reactor after 2 h enrichment in 0.1 m Na2SO4, showing the great potential of a‐PdSe2 NPs/C for practical H2O2 electrosynthesis.
Molecular Evolutionary Growth of Ultralong Semiconducting Double‐Walled Carbon Nanotubes
Authors: Gao Jun , Jiang Yaxin , Chen Sibo , Yue Hongjie , Ren He , Zhu Zhenxing , Wei Fei 
Published: November 2022

AbstractThe self‐assembling preparation accompanied with template auto‐catalysis loop and the ability to gather energy, induces the appearance of chirality and entropy reduction in biotic systems. However, an abiotic system with biotic characteristics is of great significance but still missing. Here, it is demonstrated that the molecular evolution is characteristic of ultralong carbon nanotube preparation, revealing the advantage of chiral assembly through template auto‐catalysis growth, stepwise‐enriched chirality distribution with decreasing entropy, and environmental effects on the evolutionary growth. Specifically, the defective and metallic nanotubes perform inferiority to semiconducting counterparts, among of which the ones with double walls and specific chirality (n, m) are more predominant due to molecular coevolution. An explicit evolutionary trend for tailoring certain layer chirality is presented toward perfect near‐(2n, n)‐containing semiconducting double‐walled nanotubes. These findings extend our conceptual understanding for the template auto‐catalysis assembly of abiotic carbon nanotubes, and provide an inspiration for preparing chiral materials with kinetic stability by evolutionary growth.
High‐Density Frustrated Lewis Pair for High‐Performance Hydrogen Evolution
Authors: Yu Wenhao , Zhang Yanyun , Qin Yingnan , Zhang Dan , Liu Kang , Bagliuk G. A. , Lai Jianping , Wang Lei 
Published: November 2022

AbstractIt is significant to develop catalysts with high‐density and adjacent frustrated Lewis pair (FLP) sites. Herein, a method is designed to construct FLPs on an intermetallic alloy (IMA) with regulable acidity and basicity by using the Mott–Schottky effect. By dint of the high‐density FLP site and suitable acidity and basicity on IMA, the fct‐PtCo@NC displays excellent hydrogen evolution reaction performance. The catalyst fct‐PtCo@NC, with high‐density FLP sites, exhibits a fast water adsorption and decomposition rate, which is derived from the synergistic effect of FLP‐acid sites and FLP‐base sites significantly reducing the energy barrier between the two processes. Due to the FLP‐base Pt site, fct‐PtCo@NC has the best binding energy of hydrogen intermediates, the H3O+ generated during the reaction can accumulate on it and form an acid‐like environment, and thus speed up the kinetics of the reaction. In comparison, the catalyst without FLP shows lower binding energy and the formation of H3O+ is not observed. This work opens a new horizon for the design of new‐style heterogeneous FLP catalysts and the catalysts with high‐density and adjacent FLP sites are expected to be used in a variety of multistep catalytic reactions.
Quantification and machine learning based N2O–N and CO2–C emissions predictions from a decomposing rye cover crop
Authors: Joshi Deepak R. , Clay David E. , Clay Sharon A. , Moriles‐Miller Janet , Daigh Aaron L. M. , Reicks Graig , Westhoff Shaina 
Published: November 2022

AbstractCover crops improve soil health and reduce the risk of soil erosion. However, their impact on the carbon dioxide equivalence (CO2e) is unknown. Therefore, the objective of this 2‐yr study was to quantify the effect of cover crop‐induced differences in soil moisture, temperature, organic C, and microorganisms on CO2e, and to develop machine learning algorithms that predict daily N2O–N and CO2–C emissions. The prediction models tested were multiple linear regression, partial least square regression, support vector machine, random forest (RF), and artificial neural network. Models’ performance was accessed using R2, RMSE and mean of absolute value of error. Rye (Secale cereale L.) was dormant seeded in mid‐October, and in the following spring it was terminated at corn's (Zea mays L.) V4 growth stage. Soil temperature, moisture, and N2O–N and CO2–C emissions were measured near continuously from soil thaw to harvest in 2019 and 2020. Prior to termination, the cover crop decreased N2O–N emissions by 34% (p = .05), and over the entire season, N2O–N emissions from cover crop and no cover crop treatments were similar (p = .71). Based on N2O–N and CO2–C emissions over the entire season and the estimated fixed cover crop‐C remaining in the soil, the partial CO2e were −1,061 and 496 kg CO2e ha–1 in the cover crop and no cover crop treatments, respectively. The RF algorithm explained more of the daily N2O–N (73%) and CO2–C (85%) emissions variability during validation than the other models. Across models, the most important variables were temperature and the amount of cover crop‐C added to the soil.
Precipitation and not cover crop composition influenced corn economic optimal N rate and yield
Authors: Bielenberg Hunter , Clark Jason D. , Sanyal Debankur , Wolthuizen John , Karki David , Rahal Amin , Bly Anthony 
Published: November 2022

AbstractThe effects of single species cover crops on corn (Zea mays L.) N requirement and grain yield are well studied throughout the U.S. Midwest. However, comparing cover crop mixes that include different compositions of grass and broadleaf species is limited. Fourteen corn N response experiments were conducted in South Dakota from 2018 to 2021. Fall cover crops planted after small grain harvest were mixtures of dominantly grasses, broadleaves, a 50/50 grass/broadleaf mixture, and a no cover crop control. Compared to the control, including a cover crop led to no differences in economic optimal N rate (EONR) and yield at zero N (0N) and yield at EONR 44%, 62%, and 83% of the time, respectively. As spring cover crop/residue biomass and its C and N content increased, corn yield at EONR decreased and EONR increased when including cover crops (R2 = 0.36–0.56). Including cover crops reduced EONR and resulted in a similar yield when precipitation increased above 850 mm. When differences occurred with economic return from N, including a cover crop reduced economic return in 3 site‐years (mean decrease of US$358 ha−1) and in only 1 site‐year did including a grass cover crop increase economic return from N (+US$335 ha−1). Thus, in the first year of growing cover crops (i.e., grasses, broadleaves, or a grass/broadleaf mix) before corn, growers can normally expect some differences in EONR. However, with the appropriate rate of N, yield at EONR is maintained and any economic differences from N normally minimized.
Genetic and phenotypic spectrum in the NONO‐associated syndromic disorder
Authors: Roessler Franziska , Beck Anita E. , Susie Ball , Tobias Bartolomaeus , Begtrup Amber , Biskup Saskia , Caluseriu Oana , Delanty Norman , Fröhlich Christine , Greally Marie T. , Karnstedt Maike , Klöckner Chiara , Kurtzberg Joanne , Schubert Susanna , Schulze Martin , Weidenbach Michael , Westphal Dominik S. , White Maire , Wolf Cordula M. , Zyskind Jacob , Popp Bernt , Strehlow Vincent 
Published: November 2022

AbstractThe non‐POU domain‐containing octamer‐binding (NONO) protein is involved in multiple steps of gene regulation such as RNA metabolism and DNA repair. Hemizygous pathogenic variants in the NONO gene were confirmed to cause a rare X‐linked syndromic disorder. Through our in‐house diagnostics and subsequent matchmaking, we identified six unrelated male individuals with pathogenic or likely pathogenic NONO variants. For a detailed comparison, we reviewed all published characterizations of the NONO‐associated disorder. The combined cohort consists of 16 live‐born males showing developmental delay, corpus callosum anomalies, non‐compaction cardiomyopathy and relative macrocephaly as leading symptoms. Seven prenatal literature cases were characterized by cardiac malformations. In this study, we extend the phenotypic spectrum through two more cases with epilepsy as well as two more cases with hematologic anomalies. By RNA expression analysis and structural modeling of a new in‐frame splice deletion, we reinforce loss‐of‐function as the pathomechanism for the NONO‐associated syndromic disorder.
A functional and robust super‐hydrophobic PCC coating based on the induced assembly of modified zirconium phosphate
Authors: Yin Bing , Hua Xujiang , Fan Fangyu , Qi Dongmei , Han Kailu , Hou Youqing , Hou Dongshuai 
Published: November 2022

AbstractImproving the permeability of concrete is one of the important measures to extend the service life of concrete. In addition, for marine engineering, sewerage projects, and so on, mechanical stability and microbial induced corrosion of concrete structures cannot be ignored. We demonstrated a method to prepare high‐performance super‐hydrophobic (SHP) coating using functional zirconium phosphate (ZrP) and polydopamine (PDA) as the primary materials. The micro‐nano rough structures were constructed on the concrete surface by alternating organic–inorganic assembly guided by each other. The coatings' apparent morphology, composition, and structure were analyzed using various surface analysis techniques. Based on the construction of micro‐nano rough structures by the induced assembly of functional ZrP, the silane treatment significantly improved the water contact angle of the coating (WCA = 154° ± 3°), and the coating exhibited relatively high wear resistance, reaching 135° WCA even after 30 friction cycles. The water absorption of the specimens treated with the SHP composite coating was reduced by 79% to treated with silane. Meanwhile, the SHP composite coating showed excellent antimicrobial and anti‐ice properties, due to the role of the functional ZrP and alternating organic–inorganic assembly. It was expected that the composite coating might potentially promote and accelerate applied superhydrophobic anticorrosive coating development for marine concrete durability.