bluuebunny/crossref_metadata_2025_split huggingface dataset:
Post-acute sequelae of SARS-CoV-2 with clinical condition definitions and comparison in a matched cohort
Authors: Horberg Michael A. , Watson Eric , Bhatia Mamta , Jefferson Celeena , Certa Julia M. , Kim Seohyun , Fathi Lily , Althoff Keri N. , Williams Carolyn , Moore Richard 
Published: October 2022

AbstractDisease characterization of Post-Acute Sequelae of SARS-CoV-2 (PASC) does not account for pre-existing conditions and time course of incidence. We utilized longitudinal data and matching to a COVID PCR-negative population to discriminate PASC conditions over time within our patient population during 2020. Clinical Classification Software was used to identify PASC condition groupings. Conditions were specified acute and persistent (occurring 0-30 days post COVID PCR and persisted 30–120 days post-test) or late (occurring initially 30-120 days post-test). We matched 3:1 COVID PCR-negative COVIDPCR-positive by age, sex, testing month and service area, controlling for pre-existing conditions up to four years prior; 28,118 PCR-positive to 70,293 PCR-negative patients resulted. We estimated PASC risk from the matched cohort. Risk of any PASC condition was 12% greater for PCR-positive patients in the late period with a significantly higher risk of anosmia, cardiac dysrhythmia, diabetes, genitourinary disorders, malaise, and nonspecific chest pain. Our findings contribute to a more refined PASC definition which can enhance clinical care.
Structural insight into the bulge-containing KRAS oncogene promoter G-quadruplex bound to berberine and coptisine
Authors: Wang Kai-Bo , Liu Yushuang , Li Jinzhu , Xiao Chengmei , Wang Yingying , Gu Wei , Li Yipu , Xia Yuan-Zheng , Yan Tingdong , Yang Ming-Hua , Kong Ling-Yi 
Published: October 2022

AbstractKRAS is one of the most highly mutated oncoproteins, which is overexpressed in various human cancers and implicated in poor survival. The G-quadruplex formed in KRAS oncogene promoter (KRAS-G4) is a transcriptional modulator and amenable to small molecule targeting. However, no available KRAS-G4-ligand complex structure has yet been determined, which seriously hinders the structure-based rational design of KRAS-G4 targeting drugs. In this study, we report the NMR solution structures of a bulge-containing KRAS-G4 bound to berberine and coptisine, respectively. The determined complex structure shows a 2:1 binding stoichiometry with each compound recruiting the adjacent flacking adenine residue to form a “quasi-triad plane” that stacks over the two external G-tetrads. The binding involves both π-stacking and electrostatic interactions. Moreover, berberine and coptisine significantly lowered the KRAS mRNA levels in cancer cells. Our study thus provides molecular details of ligand interactions with KRAS-G4 and is beneficial for the design of specific KRAS-G4-interactive drugs.
Performance and mechanisms of greywater treatment in a bio-enhanced granular-activated carbon dynamic biofilm reactor
Authors: Wang Ziqi , Yang Ying , Xiang Wanchen , Wu Beibei , Cui Xiaocai , Zhou Yun 
Published: October 2022

AbstractThe potential of source-diverted graywater reuse mainly relies on the efficiency and cost of graywater treatment technology. Oxygen (O2) supply and utilization rate directly determine the energy consumption and pollutants removal rate in the biological graywater treatment. This study developed a gravity flow self-supplying O2 and easy-to-maintain bio-enhanced granular-activated carbon dynamic biofilm reactor (BhGAC-DBfR) for on-site graywater treatment. Results showed that increasing of saturated/unsaturated ratio led to the continuous growth of biomass on GAC surface. Division of saturated and unsaturated zones favors the formation of aerobic-anoxic-anaerobic biofilm in the reactor. A saturated/unsaturated ratio of 1:1.1 achieved the maximum removal rate of chemical oxygen demand (COD), linear alkylbenzene sulfonates (LAS), ammonia nitrogen, and total nitrogen at 98.3%, 99.4%, 99.8%, and 83.5%, respectively. Key is that adsorption and biodegradation play important and distinct roles in the quick uptake and continuous removal of both organics and N in the system. The related genus and enzymes functional for LAS mineralization, deamination of organic N, ammonium oxidation, and nitrate respiration enabled the efficient and simultaneous removal of organics and N in the BhGAC-DBfR. This study offers a promising engineering alternative technology with great potential to achieve efficient and low-energy-input graywater treatment.
Structures of the TMC-1 complex illuminate mechanosensory transduction
Authors: Jeong Hanbin , Clark Sarah , Goehring April , Dehghani-Ghahnaviyeh Sepehr , Rasouli Ali , Tajkhorshid Emad , Gouaux Eric 
Published: October 2022

AbstractThe initial step in the sensory transduction pathway underpinning hearing and balance in mammals involves the conversion of force into the gating of a mechanosensory transduction channel1. Despite the profound socioeconomic impacts of hearing disorders and the fundamental biological significance of understanding mechanosensory transduction, the composition, structure and mechanism of the mechanosensory transduction complex have remained poorly characterized. Here we report the single-particle cryo-electron microscopy structure of the native transmembrane channel-like protein 1 (TMC-1) mechanosensory transduction complex isolated from Caenorhabditis elegans. The two-fold symmetric complex is composed of two copies each of the pore-forming TMC-1 subunit, the calcium-binding protein CALM-1 and the transmembrane inner ear protein TMIE. CALM-1 makes extensive contacts with the cytoplasmic face of the TMC-1 subunits, whereas the single-pass TMIE subunits reside on the periphery of the complex, poised like the handles of an accordion. A subset of complexes additionally includes a single arrestin-like protein, arrestin domain protein (ARRD-6), bound to a CALM-1 subunit. Single-particle reconstructions and molecular dynamics simulations show how the mechanosensory transduction complex deforms the membrane bilayer and suggest crucial roles for lipid–protein interactions in the mechanism by which mechanical force is transduced to ion channel gating.
Vigor at work mediates the effect of transformational and authentic leadership on engagement
Authors: Lopez-Zafra Esther , Pulido-Martos Manuel , Cortés-Denia Daniel 
Published: October 2022

AbstractSeveral studies have posited that authentic leadership (AL) and transformational leadership (TFL) imply ethical behaviour that can mitigate tendencies towards low engagement at work. However, there is a lack of studies analysing, for the same sample, the effect of both styles as a job resource and their effects on employees’ engagement as a means of facilitating their work goals and reducing their job demands. This study addresses this shortcoming by analysing the relations of both leadership styles to vigor, an affective construct, and engagement at work, a motivational outcome. Moreover, the possible mediation effect of vigor at work on the relationship between both leadership styles and engagement is considered. Finally, we explore the differential contributions of both styles to employees’ resources. A sample of Spanish employees (N = 215; 48.8% female) under the supervision of a direct leader responded concerning the TFL and AL of their closest supervisor and their own vigor at work and engagement. Our results show that vigor increases the effect of both leadership styles on engagement. Moreover, TFL, to a greater extent than AL, relates to higher engagement. Thus, vigor as an affective dimension mediates the effect of positive leadership on engagement. This study considers, for the same sample, the effects of two related, albeit different, leadership styles. The results indicate that AL and TFL are positively perceived by employees as creating a climate of energy that acts as a resource (both organizational and personal). Practitioners could enhance employees’ vigor at work and engagement by promoting these two leadership styles.
Loss of miR-936 leads to acquisition of androgen-independent metastatic phenotype in prostate cancer
Authors: Edachery Sarathkumar , Patil Prakash , Mohan Rajashekar , Aradhya Bhuvanesh , Shetty Jayaprakash , Kabekkodu Shama Prasada , Santra Manas Kumar , Gonchigar Sathisha Jayanna , Shetty Praveenkumar 
Published: October 2022

AbstractProstate cancer (PCa) progresses from a hormone-sensitive, androgen-dependent to a hormone-refractory, androgen-independent metastatic phenotype. Among the many genes implicated, ANXA2, a calcium-dependent phospholipid binding protein, has been found to have a critical role in the progression of PCa into more invasive metastatic phenotype. However, the molecular mechanisms underlying the absence of ANXA2 in early PCa and its recurrence in advanced stage are yet unknown. Moreover, recent studies have observed the deregulation of microRNAs (miRNAs) are involved in the development and progression of PCa. In this study, we found the down-regulation of miR-936 in metastatic PCa wherein its target ANXA2 was overexpressed. Subsequently, it has been shown that the downregulation of miRNA biogenesis by siRNA treatment in ANXA2-null LNCaP cells could induce the expression of ANXA2, indicating the miRNA mediated regulation of ANXA2 expression. Additionally, we demonstrate that miR-936 regulates ANXA2 expression by direct interaction at coding as well as 3′UTR region of ANXA2 mRNA by luciferase reporter assay. Furthermore, the overexpression of miR-936 suppresses the cell proliferation, cell cycle progression, cell migration, and invasion abilities of metastatic PCa PC-3 cells in vitro and tumor forming ability in vivo.These results indicate that miR-936 have tumor suppressor properties by regulating the over expression of ANXA2 in hormone-independent metastatic PCa. Moreover, our results suggest that this tumor suppressor miR-936 could be developed as a targeted therapeutic molecule for metastatic PCa control and to improve the prognosis in PCa patients.
Bacterial infiltration and detorque at the implant abutment morse taper interface after masticatory simulation
Authors: Bella Ana Paula Granja Scarabel Nogueira , Tuzita Alessandra Sayuri , Suffredini Ivana Barbosa , Kojima Alberto Noriyuki , Giovani Elcio Magdalena , Mesquita Alfredo Mikail Melo 
Published: October 2022

AbstractThis study evaluated the bacterial infiltration and the detorque of indexed and non-indexed abutments of Morse taper implants (MTI) after mechanical cycling (MC). 40 MTI were distributed into four groups: IIA (indexed implant abutments); NIIA (non-indexed implant abutments); IIAMC (indexed implant abutments submitted to MC); NIIAMC (non-indexed implant abutments submitted to MC), which were carried out under one million 5 Hz frequency and 3 Bar pressure. After mechanical cycling, all groups were immersed in a bacterial solution in Brain Heart Infusion Agar. After detorque, the bacteria infiltration was evaluated by counting the colony-forming units. For the bacterial infiltration, analysis was applied to the Kruskal–Wallis test (p = 0.0176) followed by Dunn’s test. For the detorque analysis, the two-way repeated-measures ANOVA was applied, followed by the Tukey’s test (p < 0.0001). Bacteria infiltration was highly observed in NIIA (p = 0.0027) and were absent in IIAMC and NIIAMC. The detorque values for IIA (19.96Ncm ± 0.19Ncm), NIIA (19.90Ncm ± 0.83Ncm), and NIIAMC (19.51Ncm ± 0,69Ncm) were similar and remained close to the initial value, while IIAMC (55.2Ncm ± 2.36Ncm) showed an extremely significant torque value increase (p < 0.0001). The mechanical cycling resulted in mechanical sealing of the implant-abutment interface, preventing bacterial infiltration in the indexed and non-indexed specimens, and increasing the detorque strength in the group of indexed abutments.
Anticoccidial and immunogenic effectivity of encapsulated organic acids and anticoccidial drugs in broilers infected with Eimeria spp.
Authors: Nouri Ali 
Published: October 2022

AbstractThe study was conducted to consider the anticoccidial and immunogenic effectivities of encapsulated organic acids and anticoccidial drugs in broilers reared on a reused litter infected with Eimeria spp. for simulating in-field exposure to avian coccidiosis. 525 mixed-sex one-day-old broiler chicks (Ross 308) were used in a 2 × 3 factorial experiment as a completely randomized design with seven experimental groups and five replicates of 15 chicks. The seven experimental groups were included: negative (uninfected; T1) and positive (infected; T2) control groups fed a diet without additive, and other infected groups (T3–T7) fed diets supplemented with 0.05% maduramicin, 0.02% diclazuril, 0.1% EOAs, 0.05% maduramicin and 0.1% EOAs, 0.02% diclazuril and 0.1% EOAs. During the experimental period, the evaluated parameters were European production efficiency factor (EPEF; at 22 days of age (d)), oocyst output per gram feces (OPG; at different ages), oocyst reduction rate (ORR; at 22-d), survival rate (SR; at 22-d), caecal lesion score (CLS at 22-d), sporulation percentage (SP; by in vitro anticoccidial tests), bloody diarrhea (BD; by scoring the bloody feces each morning from 13 to 31-d), immunity (humoral test at 28 and 35-d and cell-mediated test at 22-d), goblet cells analysis of the jejunum (GC; at 22-d) and anti-coccidiosis index (ACI; at 22-d). EOAs and anticoccidials, especially their simultaneous feeding improved (P < 0.05) broiler’s EPEF, SR, OPG, ORR, SP, CLS, immunity and BD (scored). ACI was improved (P < 0.05) by EOAs more than anticoccidials (marked vs. moderate). The highest ACI was significantly observed in EOAs + diclazuril group. EOAs as a safe alternative had more intensive anticoccidial and immunogenic properties and increased the anticoccidial drugs’ effectiveness, especially diclazuril in Eimeria spp-infected broilers.
Effects of multi-directional step exercise with weight-shifting as an adjunct to conventional exercises on balance and gait in stroke patients
Authors: Shrestha Rakesh , Sandesh T. S. , Jalal Zainab , Nuhmani Shibili , Alghadir Ahmad H. , Khan Masood 
Published: October 2022

AbstractStroke patients have gait dysfunctions that affect their activities of daily living. Stroke patients should be able to take multi-directional steps as it is necessary to achieve an independent gait. The study aimed to examine the effects of multi-directional step exercises (MSE) along with weight-shifting as an adjunct to conventional therapeutic exercises (CTE) on functional gait performance and balance in patients with stroke. Twenty-four stroke patients (mean age 56.75 years) participated in the study and were divided into experimental and control groups. The experimental group (EG) included MSE along with weight shifting and CTE. The control group (CG) included only CTE. Treatment intervention lasted for 4 weeks. Gait and balance were measured using the functional gait assessment (FGA) and the berg balance scale (BBS), respectively. EG showed a significant improvement (p = 0.000) in both the BBS and FGA scores. In CG, a significant improvement (p = 0.000) was observed only in FGA scores. EG showed a greater improvement in scores of BBS (p = 0.000) and FGA (p = 0.000) than CG. Four weeks of MSE in conjunction with CTE were more effective in improving balance and functional gait performance compared to CTE alone in the selected stroke population.
A semi-analytical passive strategy to examine the water-ethylene glycol (50:50)-based hybrid nanofluid flow over a spinning disk with homogeneous–heterogeneous reactions
Authors: Algehyne Ebrahem A. , Altaweel Nifeen H. , Saeed Anwar , Dawar Abdullah , Ramzan Muhammad , Kumam Poom 
Published: October 2022

AbstractScientists and researchers are much interested in studying graphene and silver nanoparticles for the enhancement of heat transport due to their extensive variety of applications in different areas of industrial and engineering such as drug delivery, medical devices, ultra-light, excellent electrical conductivity, strong medical strength, health care, consumer, food, etc. Therefore, in the existing investigation, the MHD flow of a mixed convective hybrid nanoliquid with graphene and silver nanoparticles past a rotating disk is considered. Water and ethylene glycol (50:50) is used as a base liquid in the existing model. The mechanism for heat transport is computed with the existence of thermal radiation and thermal convective condition. Homogeneous and heterogeneous chemical reactions are assumed in the flow behavior. The mathematical formulation of the proposed problem is based on the nonlinear PDEs which are then transformed to nonlinear ODEs by manipulating the appropriate similarity transformation. The simulation of the existing problem has been performed with the help of the homotopy analysis technique. The outcomes of the different flow parameters on the velocities, temperature, concentration, skin friction coefficient, and Nusselt number of the hybrid nanofluid are attained via graphs and tables. Some significant results from the existing problem demonstrate that the rate of heat transport is greater for the thermal Biot number and nanoparticles volume fraction. Further, it is noticed that the velocity of the liquid particles becomes lower for a higher magnetic field parameter.
Alterations of brain microstructures in a mouse model of prenatal opioid exposure detected by diffusion MRI
Authors: Grecco Gregory G. , Shahid Syed Salman , Atwood Brady K. , Wu Yu-Chien 
Published: October 2022

AbstractGrowing opioid use among pregnant women is fueling a crisis of infants born with prenatal opioid exposure. A large body of research has been devoted to studying the management of opioid withdrawal during the neonatal period in these infants, but less substantive work has explored the long-term impact of prenatal opioid exposure on neurodevelopment. Using a translationally relevant mouse model of prenatal methadone exposure (PME), the aim of the study is to investigate the cerebral microstructural differences between the mice with PME and prenatal saline exposure (PSE). The brains of eight-week-old male offspring with either PME (n = 15) or PSE (n = 15) were imaged using high resolution in-vivo diffusion magnetic resonance imaging on a 9.4 Tesla small animal scanner. Brain microstructure was characterized using diffusion tensor imaging (DTI) and Bingham neurite orientation dispersion and density imaging (Bingham-NODDI). Voxel-based analysis (VBA) was performed using the calculated microstructural parametric maps. The VBA showed significant (p < 0.05) bilateral alterations in fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), radial diffusivity (RD), orientation dispersion index (ODI) and dispersion anisotropy index (DAI) across several cortical and subcortical regions, compared to PSE. Particularly, in PME offspring, FA, MD and AD were significantly higher in the hippocampus, dorsal amygdala, thalamus, septal nuclei, dorsal striatum and nucleus accumbens. These DTI-based results suggest widespread bilateral microstructural alterations across cortical and subcortical regions in PME offspring. Consistent with the observations in DTI, Bingham-NODDI derived ODI exhibited significant reduction in PME offspring within the hippocampus, dorsal striatum and cortex. NODDI-based results further suggest reduction in dendritic arborization in PME offspring across multiple cortical and subcortical regions. To our best knowledge, this is the first study of prenatal opioid exposure to examine microstructural organization in vivo. Our findings demonstrate perturbed microstructural complexity in cortical and subcortical regions persisting into early adulthood which could interfere with critical neurodevelopmental processes in individuals with prenatal opioid exposure.
Reproductive performance and milk yield of rabbits fed diets supplemented with garden cress (Lepidium sativum) seed
Authors: El-Gindy Yassmine M. , Zahran Soliman M. , Ahmed Mohamed H. , Idres Azza Y. , Aboolo Safaa H. , Morshedy Sabrin A. 
Published: October 2022

AbstractRecently, phytochemicals in plants have evoked interest as sources of naturally beneficial substances and as alternatives to antimicrobials. Based on these benefits, it was hypothesized that garden cress (GC; Lepidium sativum) supplementation could overcome the negative impacts of severe heat stress on the reproductive and lactation performance, physiological parameters, and antioxidant status of rabbits. Twenty-four mature V-line does (6 months old) with an average body weight of 3.65 ± 0.54 kg were randomly assigned into four homogenously equal groups (n = 6) according to the level of supplemented GC seeds. Rabbits in the control group were fed a basal diet without GC seeds (GC 0), the other three treatment groups (GC 3, GC 4.5, and GC 6) were fed a basal diet supplemented with GC seeds at levels 3, 4.5 and 6%, respectively. Investigations revealed that the inclusion of 3% GC enhanced the litter weight of heat-stressed rabbits on the 7th, 14th, and 21st days. Furthermore, treatment with 3% and 6% GC seeds increased the milk yield on the 28th day. the most of lipid profile parameters, such as cholesterol, triglyceride, high-density lipoprotein (HDL), low-density lipoprotein (LDL), serum urea levels, and antioxidant status improved in rabbits supplemented with 6% GC. In conclusion, the dietary supplemention of GC seed at 6% increased milk yield at 28th day “weaning age”, consequently, improved the blood lipid profile and antioxidant status. Further studies should be conducted to commercialize theusage of garden cress seeds as a supplement in rabbits.
Infants expect agents to minimize the collective cost of collaborative actions
Authors: Mascaro Olivier , Csibra Gergely 
Published: October 2022

AbstractThis paper argues that human infants address the challenges of optimizing, recognizing, and interpreting collaborative behaviors by assessing their collective efficiency. This hypothesis was tested by using a looking-time study. Fourteen-month-olds (N = 32) were familiarized with agents performing a collaborative action in computer animations. During the test phase, the looking times were measured while the agents acted with various efficiency parameters. In the critical condition, the agents’ actions were individually efficient, but their combination was either collectively efficient or inefficient. Infants looked longer at test events that violated expectations of collective efficiency (p = .006,d = 0.79). Thus, preverbal infants apply expectations of collective efficiency to actions involving multiple agents.
Characteristics of drug-related problems and pharmacist’s interventions in hospitalized patients in Thailand: a prospective observational study
Authors: Deawjaroen Kulchalee , Sillabutra Jutatip , Poolsup Nalinee , Stewart Derek , Suksomboon Naeti 
Published: October 2022

AbstractDrug-related problems (DRPs) are a major health concern. A better understanding of the characteristics of DRPs throughout the hospital stay may help to tailor pharmaceutical care services (PCS). This study aims to describe the characteristics of DRPs and to compare DRP pattern in different stages of hospital stay. DRPs were identified by clinical pharmacists as part of their routine services. Pharmacist assessed causality, severity and preventability of DRP. A total of 316 preventable DRPs occurred in 257 patients with the median of 1 (rang 1–3) DRPs per patient. 46.8% of DRPs occurred at discharge than at other stages. The most frequent cause of DRP was no drug treatment in spite of existing indication, accounting for 32.3% of all DRPs. No drug treatment with existing indication was detected frequently at discharge (56.1%) compared with other stages (p-value < 0.001). The common intervention to physician was starting a drug (34.0%) and the acceptance rate was 95.8%. DRPs in hospitalized patients occur at any stage of the hospital stay. Systematic identification of DRP characteristics enables pharmacists to tailor optimal type of PCS required and hence improve patient safety.