bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic review of interruption and redirection procedures for autistic individuals
Authors: Ledford Jennifer R. , Trump Cary , Chazin Kate T. , Windsor Sienna A. , Eyler Paige Bennett , Wunderlich Kara 
Published: August 2022

AbstractInterruption and redirection (IR) procedures involve systematically disrupting an undesirable behavior and prompting an individual to engage in an alternative behavior. These procedures have been frequently assessed for reducing repetitive behaviors for autistic individuals. The primary purpose of this review was to assess IR interventions to determine whether outcomes vary according to intervention characteristics, dependent variable types, design quality, or publication status. A secondary purpose was to assess the extent to which generalization and maintenance outcomes were measured and whether characteristics of these conditions were related to outcomes. IR interventions were moderately successful for reducing repetitive behaviors but less effective for improving corollary variables such as appropriate vocalizations and functional engagement. Generalization and maintenance outcomes, when measured, were poor. Results indicate the need for alternative or augmentative procedures that focus on engagement in meaningful interactions and activities.
Hybrid cell constructs consisting of bioprinted cell‐spheroids
Authors: Kim WonJin , Kim GeunHyung 
Published: August 2022

AbstractBioprinted cell constructs have been investigated for regeneration of various tissues. However, poor cell–cell interactions have limited their utility. Although cell‐spheroids offer an alternative for efficient cell–cell interactions, they complicate bioprinting. Here, we introduce a new cell‐printing process, fabricating cell‐spheroids and cell‐loaded constructs together without preparation of cell‐spheroids in advance. Cells in mineral oil droplets self‐assembled to form cell‐spheroids due to the oil‐aqueous interaction, exhibiting similar biological functions to the conventionally prepared cell‐spheroids. By controlling printing parameters, spheroid diameter and location could be manipulated. To demonstrate the feasibility of this process, we fabricated hybrid cell constructs, consisting of endothelial cell‐spheroids and stem cells loaded decellularized extracellular matrix/β‐tricalcium phosphate struts for regenerating vascularized bone. The hybrid cell constructs exhibited strong angiogenic/osteogenic activities as a result of increased secretion of signaling molecules and synergistic crosstalk between the cells.
Cytotoxic Effect of the Essential oils from Erigeron Canadensis L. on Human Cervical Cancer HeLa Cells in Vitro
Authors: Si Chaojin , Ou Yangsong , Ma Danwei , Hei Lei , Wang Xiaoyan , Du Runyuan , Yang Hongjun , Liao Ying , Zhao Jiayuan 
Published: August 2022

AbstractErigeron Canadensis L. (E. canadensis) is a widely distributed invasive weed species in China. Potentially anti‐cancer qualities may exist in its essential oils (EOs). The purpose of this study was to analyze the components of the EOs of E. canadensis and their effects on the normal liver cell lines L02 and the human cervical cancer cell lines HeLa. The EOs from the upper region of E. canadensis were prepared, its components were identified by GC/MS. Cell viability, cell morphology observation, AO/EB dual fluorescence staining assay, flow cytometry, mitochondrial membrane potential, western blot, caspase inhibitor test, and oxidative stress tests were used to investigate the impact of the EOs on HeLa cells. Network pharmacological analysis was employed to study the potential mechanism of the EOs in the treatment of cervical cancer. According to the findings, the EOs had 21 chemical components, of which limonene made up 65.68 %. After being exposed to the EOs, the cell viability of HeLa and L02 dramatically declined. The inhibition of EOs was more effective than that of limonene when used in an amount equivalent to that in the EOs. L02 cells were less susceptible to the cytotoxicity of EOs than HeLa cells were. Furthermore, EOs altered the cell cycle in HeLa cells and caused oxidative stress and apoptosis. Compared with the control group, the reactive oxygen species (ROS) levels increased in HeLa cells at first and then decreased, total superoxide dismutase (SOD) and catalase (CAT) activities in HeLa cells significantly decreased. G1 phase cells decreased whereas G2/M phase cells increased. The rate of apoptosis rose. Reduced mitochondrial membrane potential and Caspase‐3, −9, and −12 protein expression were both observed. Nerolidol, dextroparaffinone, and α‐pinene were shown to be the primary components for the suppression of HeLa cells, according to the results of the prediction of pharmacologic targets. In conclusion, findings of this study indicated the EOs may have the potential to curb the growth of cervical cancer cells. Further research is needed to explore the in vivo effect of EOs.
Risk and tropism of central nervous system (CNS) metastases in patients with stage II and III cutaneous melanoma
Authors: Johannet Paul , Simons Morgan , Qian Yingzhi , Azmy Nadine , Mehnert Janice M. , Weber Jeffrey S. , Zhong Judy , Osman Iman 
Published: August 2022

BackgroundRecent data suggest that patients with stage III melanoma are at high risk for developing central nervous system (CNS) metastases. Because a subset of patients with stage II melanoma experiences worse survival outcomes than some patients with stage III disease, the authors investigated the risk of CNS metastasis in stage II melanoma to inform surveillance guidelines for this population.MethodsThe authors examined clinicopathologic data prospectively collected from 1054 patients who had cutaneous melanoma. The χ2 test, the cumulative incidence, and Cox multivariable regression analyses were performed to evaluate the association between baseline characteristics and the development of CNS metastases.ResultsPatients with stage III melanoma had a higher rate of developing brain metastases than those with stage II melanoma (100 of 468 patients [21.4%] vs. 82 of 586 patients [14.0%], respectively; p = .002). However, patients who had stage IIC melanoma had a significantly higher rate of isolated first recurrences in the CNS compared with those who had stage III disease (12.1% vs. 3.6%; p = .002). The risk of ever developing brain metastases was similarly elevated for patients who had stage IIC disease (hazard ratio [HR], 3.16; 95% CI, 1.77–5.66), stage IIIB disease (HR, 2.83; 95% CI, 1.63–4.91), and stage IIIC disease (HR, 2.93; 95% CI, 1.81–4.74), and the risk was highest in patients who had stage IIID disease (HR, 8.59; 95% CI: 4.11–17.97).ConclusionsPatients with stage IIC melanoma are at elevated risk for first recurrence in the CNS. Surveillance strategies that incorporate serial neuroimaging should be considered for these individuals until more accurate predictive markers can be identified.
Coaxial Hard Carbon‐coated Carbon Nanotubes as Anodes for Sodium‐ion Batteries
Authors: Feng Shan , Chen Jialu , Niu Xingyu , Feng Xiaomiao , Zhao Jin 
Published: August 2022

AbstractAlthough being advantageous, the sodium‐ion storage capability of hard carbon would be better if its electrical conductivity could be improved. Herein, 3D hard carbon‐coated carbon nanotubes (CNTs@C) with interconnected conductive networks are fabricated by inducing hard carbon uniformly growth on CNTs. Compared with the isolated hard carbon spheres, the interconnected wire shape of CNTs@C reduces the ion transfer distance and increases the conductivity of hard carbon. By optimizing the thickness of the hard carbon layer and annealing temperature, the optimized CNTs@C displays a reversible specific capacity of 275.9 mAh g−1 at 0.1 A g−1 as anode material for SIBs. Furthermore, the material shows remarkable rate capability (102.8 mAh g−1 at 5 A g−1) and long‐life cycling performance (∼100% capacity retention after 1000 cycles at 5 A g−1). The results of this study provide an effective route for designing novel carbon‐based composite materials as anodes for SIBs.
How low can you go? Introducing SeXY: sex identification from low‐quantity sequencing data despite lacking assembled sex chromosomes
Authors: Cabrera Andrea A. , Rey‐Iglesia Alba , Louis Marie , Skovrind Mikkel , Westbury Michael V. , Lorenzen Eline D. 
Published: August 2022

AbstractAccurate sex identification is crucial for elucidating the biology of a species. In the absence of directly observable sexual characteristics, sex identification of wild fauna can be challenging, if not impossible. Molecular sexing offers a powerful alternative to morphological sexing approaches. Here, we present SeXY, a novel sex‐identification pipeline, for very low‐coverage shotgun sequencing data from a single individual. SeXY was designed to utilize low‐effort screening data for sex identification and does not require a conspecific sex‐chromosome assembly as reference. We assess the accuracy of our pipeline to data quantity by downsampling sequencing data from 100,000 to 1000 mapped reads and to reference genome selection by mapping to a variety of reference genomes of various qualities and phylogenetic distance. We show that our method is 100% accurate when mapping to a high‐quality (highly contiguous N50 > 30 Mb) conspecific genome, even down to 1000 mapped reads. For lower‐quality reference assemblies (N50 < 30 Mb), our method is 100% accurate with 50,000 mapped reads, regardless of reference assembly quality or phylogenetic distance. The SeXY pipeline provides several advantages over previously implemented methods; SeXY (i) requires sequencing data from only a single individual, (ii) does not require assembled conspecific sex chromosomes, or even a conspecific reference assembly, (iii) takes into account variation in coverage across the genome, and (iv) is accurate with only 1000 mapped reads in many cases.
Electrochemical Lossy Mode Resonance‐based Fiber Optic Sensing for Electroactive Species
Authors: Okazaki Takuya , Yoshioka Masaki , Orii Tatsuya , Taguchi Akira , Kuramitz Hideki , Watanabe Tomoaki 
Published: August 2022

AbstractWe present an electrochemical‐lossy mode resonance (LMR) sensing method that detects refractive indices and electroactive species. The LMR peaks of indium‐tin‐oxide in the transmittance‐wavelength spectra were significantly shifted as the applied potential between 1.0 and −0.5 V at 209 nm/V. The modulation was exploited for sensing the refractive index and electroactive species (ferrocyanide and methylene blue) in two ways: peak‐wavelength tracking and potential scanning. The potential‐scanning technique produced clear potential LMR peaks in the transmittance‐potential spectra for the first time, which were corresponded to the external refractive index. Meanwhile, the limits of detection of ferrocyanide and methylene blue were 7.5 and 25.3 μM, respectively, in peak‐wavelength tracking and 18.2 and 20.8 μM, respectively, in the potential scanning technique.
Exploration of the phytohormone regulation of energy storage compound accumulation in microalgae
Authors: Shah Saud , Li Xiuling , Jiang Zhaoyu , Fahad Shah , Hassan Shah 
Published: August 2022

AbstractMicroalgal energy storage compounds (carbohydrates, lipids, etc.) can serve as renewable feedstocks for biofuels and biobased chemicals. Traditional methods of inducing the accumulation of energy storage compounds in microalgae, such as abiotic stress (high light intensity, high salinity, nutrient limitation, heavy metals, etc.), can affect the growth of microalgae and limit their efficient accumulation of energy storage materials. Plant hormones are a class of small molecular substances that act as chemical messengers to coordinate plant cell activities and regulate the physiological and metabolic activities of microalgae, including promoting microalgal cell proliferation, improving stress resistance, and enhancing photosynthetic activity, thereby increasing algal biomass and lipid, chlorophyll and protein content. This paper reviews the recent research progress on regulation of the accumulation of energy storage compounds in microalgae by adding exogenous plant hormones combined with abiotic stress, discusses the mechanism of plant hormones regarding the accumulation of energy storage compounds in microalgae, and proposes future research needs.
A height function based momentum balance model to simulate contact angle dynamics with hysteresis
Authors: Li Jing , Lin Zhiliang 
Published: August 2022

AbstractWe report a combined method to deal with the contact angle dynamics with hysteresis. The momentum balance model is applied to obtain the transient contact angle by balancing the inertia and the capillary force where the curvatures are estimated by the height function at the contact line. This integrated approach provides a great convenience that no need for a prior knowledge of the contact angle, and possesses the good property of the sharp interface approximation. In order to facilitate the wider use of the present method, we incorporate a dynamic contact line model to estimate the contact angle when the contact line starts to move. This combination plus the hysteresis region will take the ability to solve most of problems related to wetting with more physical sense. This proposed model is finally validated by the droplet equilibrium, spreading, and sliding tests.
Analysis of protective effects of Rosa Roxburghii Tratt fruit polyphenols on lipopolysaccharide‐induced acute lung injury through network pharmacology and metabolomics
Authors: Tang Li , Zhang Shuo , Zhang Min , Wang Peng‐Jiao , Liang Gui‐You , Gao Xiu‐Li 
Published: August 2022

AbstractAcute lung injury (ALI) is a respiratory disease with high morbidity and mortality rates and is the primary cause of death in children and the elderly around the world. The use of Chinese foods in the complementary and alternative treatment of ALI has attracted more and more attention. This study aimed to explore the anti‐ALI activity of Chinese functional foods Rosa roxburghii Tratt fruit polyphenols (RRTP). RRTP was administered to lipopolysaccharide‐induced ALI mice, and its protective effects were comprehensively evaluated by lung histopathological examination, wet/dry (W/D) ratio, and cytokine production. Metabolomics analysis was used to identify the differential metabolites and metabolic pathways in plasma, and molecular docking and systemic biology‐based network pharmacology assay were performed to explore the active components and potential therapeutic targets. The results indicated that RRTP significantly attenuated the severity of pathological changes and pulmonary capillary permeability. Furthermore, RRTP limited the increase in tumor necrosis factor alpha (TNF‐α), interleukin 1β (IL‐1β), and interleukin 6 (IL‐6) levels and the decrease in interleukin 10 (IL‐10) levels in ALI mice. Metabolomics studies revealed that RRTP markedly affected 19 different metabolites, three amino acid metabolism pathways, and sphingolipid metabolism. Moreover, network pharmacology identified AKT1 (AKT serine/threonine kinase 1), TP53, IL‐6, VEGFA (vascular endothelial growth factor A), and TNF (tumor necrosis factor) as the most promising target proteins, while quercetin, luteolin, and kaempferol were the core active components of RRTP. This study investigated the complex mechanisms of RRTP against ALI for the first time, and provided a foundation for the application of RRTP as a functional food, facilitating the research of nutritional food additives for the adjuvant treatment of ALI.
Early meteorological records from Corrientes and Bahía Blanca, Argentina: Initial ACRE‐Argentina data rescue and related activities
Authors: Lakkis Susan Gabriela , Canziani Pablo O. , Rodriquez Joaquín O. , Yuchechen Adrián E. 
Published: August 2022

AbstractThe international Atmospheric Circulation Reconstructions over the Earth (ACRE) initiative works to recover global climate history and build an accessible databank, with data from the past 250 years of terrestrial and marine surface. Argentina is part of that effort with ACRE Argentina recovering data from various sources that have been recorded throughout the country since the 19th century. In this paper, weather observations transcribed from the original records into digital form and taken every day during the years 1860–1879 at Bahía Blanca and from 1873–1886 at Corrientes are presented. The digitization was carried out through the project Meteorologum ad Extremum Terrae (MET) launched in the Zooniverse interface, which is currently working with near 900 citizen scientist volunteers per day. The present analysis corresponds to data retrieved from the collection ‘Anales de la Oficina Meteorológica Argentina’ and contains information on atmospheric pressure, air temperature, relative humidity, cloud cover, cloud types, wind direction, wind speed, rainfall and weather remarks. For the present analysis, only temperature and pressure values, measured in °C and mm of Hg, respectively, were considered, after a quality control of the digitization process was applied. Data values were tested and used to rebuild the time series of both places and correlation with SOI index and monthly pressure values for both places was tested using Spearman correlation. Results show that the influence of El Niño episode during 1877–1878 can be found in the pressure values at Corrientes.
Public contracting of home respiratory care services through an electronic quasi‐market model
Authors: Valadares Tavares Luís , Arruda Pedro 
Published: August 2022

AbstractRespiratory diseases are becoming a major challenge in health systems as they cover expanding and diversified pathologies such as chronic obstructive pulmonary disease, chronic bronchitis, emphysema and other types increasing at a fast rate as it is the case of sleep apnoea. Nowadays, most of these pathologies can be treated by home respiratory care services using advanced medical equipment as well as efficient assistance and monitoring services supported by digital technologies. However, the implementation of home respiratory care services implies flexible public contracting of such services to achieve equity conditions of access and to cope with their technological nature, their continuous processes of innovation and their time dependent demand. Unfortunately, traditional procurement processes are not able to meet these challenges explaining their frequent low rates of success and so an alternative procurement approach is proposed in this paper based on an electronic quasi‐market model. This new model was successfully applied in Portugal and the evaluated results are also discussed herein.
Factors associated with pediatric genetic counselors' practices related to bullying screening
Authors: Ginter Jacob A. , Lepard‐Tassin Tiffany , Selig James P. , Danylchuk Noelle R. 
Published: August 2022

AbstractBullying is reported in around 20% of children according to the US Department of Education and has been reported in the histories of individuals with genetic disorders. To our knowledge, there has never been a study surveying whether genetic counselors screen their pediatric patients for bullying. This is despite guidelines that pediatric healthcare providers should screen for bullying. The purpose of this study was to assess North American genetic counselors who see pediatric patients and enquire about their practices, attitudes, self‐confidence, knowledge, and potential training needs in relation to bullying screening. In an anonymous online survey, 139 genetic counselors from the United States and Canada completed a modified version of the previously validated Healthcare Providers Practices, Attitudes, Self‐Confidence, and Knowledge (HCP‐PACK) instrument. Among our participant population, 85% reported they did not screen for bullying. This is despite no statistically significant difference in the amount of reported time spent on either initial or follow‐up appointments between those who did or did not screen. Those who screened for bullying among their pediatric patients were more likely to view bullying as a healthcare problem (as measured on the attitude subscale) (t[135] = −2.07, p = 0.04) and had greater confidence in their ability to know how to assess for bullying (as measured on the self‐confidence subscale) (t[135] = −2.90, p = 0.004) compared with participants who did not screen for bullying. Responses from genetic counselors who screened their patients for bullying demonstrated how screening for bullying can be aligned with the American Board of Genetic Counseling practice‐based competencies. Even though the majority of participants did not view screening for bullying as a genetic counselor's role, 82.5% agreed that bullying was a healthcare problem and 63.6% thought genetic counselors should have additional educational opportunities to learn about bullying. Evidence‐based guidance is needed to help genetic counselors interested in including screening for bullying in their practice.
Comparison of long‐term prognosis between non‐obese and obese patients with non‐alcoholic fatty liver disease
Authors: Iwaki Michihiro , Kessoku Takaomi , Tanaka Kosuke , Ozaki Anna , Kasai Yuki , Yamamoto Atsushi , Takahashi Kota , Kobayashi Takashi , Nogami Asako , Honda Yasushi , Ogawa Yuji , Imajo Kento , Oyamada Shunsuke , Kobayashi Noritoshi , Aishima Shinichi , Saito Satoru , Nakajima Atsushi , Yoneda Masato 
Published: August 2022

AbstractBackground and AimNon‐alcoholic fatty liver disease (NAFLD) can progress in non‐obese patients as in obese patients. Reports on long‐term prognosis in non‐obese NAFLD patients are controversial. Therefore, we aimed to examine the long‐term prognosis of non‐obese patients with NAFLD.MethodsThis single‐center, retrospective cohort study enrolled biopsy‐proven non‐obese and obese NAFLD patients between January 2002 and December 2011 and followed them up until 31 March 2021, for death and clinical events (cardiovascular and liver‐related events and extrahepatic cancers).ResultsOf the 223 NAFLD patients, 58 (26.0%) were non‐obese. Compared with obese patients, they had a lower fibrosis stage (0.8 ± 0.80 vs 1.2 ± 0.91; P = 0.004), milder lobular inflammation (0.9 ± 0.7 vs 1.1 ± 0.7; P = 0.02), and significantly lower serum creatinine, total bilirubin, ferritin, and type IV collagen 7S and higher high‐density lipoprotein levels. After a median follow‐up of 8.9 years, no significant difference was noted in mortality between the two groups (2 [3.4%] non‐obese vs 5 [3.0%] obese; log‐rank test, P = 0.63). Twelve patients (20.7%) in the non‐obese group and 32 (19.4%) in the obese group had clinical events. Although the obese group had a higher incidence of clinical events during the first 10 years of follow‐up, the non‐obese group had a higher incidence after that (log‐rank test, P = 0.67). The non‐obese group had a high incidence of malignancy (9 [15.5%] non‐obese vs 14 [8.3%] obese; P = 0.13).ConclusionNon‐obese NAFLD does not necessarily have a good prognosis, and some cases have a poor prognosis such as extrahepatic cancers. Further validation is required in the future.