bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Cell-Free DNA-Based Next-Generation Sequencing for Identifying Pathogens in Bacteremia Patients
Authors: Zhang Chaoqin , Cheng Hang , Zhao Yuxi , Chen Jinlian , Li Meng , Yu Zhijian , Sun Xiang , Li Peiyu , Shang Yongpeng , Ma Jinmin , Zheng Jinxin 
Published: November 2022

Abstract
Rapid detection of bloodstream pathogens would greatly facilitate clinicians to make precise antimicrobial treatment in patients with bacteremia. In this study, 114 plasma samples were collected from patients with identified or suspected bacteremia, and pathogens were detected by the conventional blood culture (BC) and cell-free DNA metagenomics next-generation sequencing (cfDNA mNGS). The present study indicated that 76% (38/50) of positive conventional blood culture (BC+ group) patients were positively detected by cfDNA mNGS, and only 4% were mismatched between cfDNA mNGS and conventional bacteria culture. Pathogens in 32.8% of suspected bacteremia patients with negative conventional blood culture (BC– group) were determined accurately by cfDNA mNGS combined with analyzing the patients’ clinical manifestations. Escherichia coli and Klebsiella pneumoniae were the most detected pathogens in identified bacteremia patients by cfDNA mNGS. 76.2% (16/21) of E. coli and 92.3% (12/13) of K. pneumoniae in bacteremia patients were identified by conventional blood cultures that were also detected by cfDNA mNGS. This study demonstrated that genomic coverage of E. coli and K. pneumoniae were more often detected in BC+ group patients and genomic coverage of Acinetobacter johnsonii and Paucibacter sp. KCTC 42545 was more often detected in BC– group patients. In conclusion, cfDNA mNGS could rapidly and precisely provide an alternative detection method for the diagnosis of bacteremia.
Pengaruh Sales Promotion Terhadap Keputusan Membeli Produk UMKM Di Kota Palembang dengan Brand Image dan Brand Quality Product Sebagai Mediasi
Authors: Elpanso Efan , Helmi Sulaiman 
Published: November 2022

Efforts to increase UMKM product sales necessitate new marketing, sales, and models capable of sustaining small business growth and development. This study examines empirical evidence of the role of brand image and product quality as a mediator of the influence of Sales Promotionon on purchasing decisions of UMKM products in Palembang. A survey with a cross-sectional approach is used in this type of research. The population of the UMKM consumer research in Palembang City. The sample size was 220 people, and the sampling technique was simple random sampling. A questionnaire was used as the research instrument. Structural Equation Modeling was used to analyze data (SEM). The study's findings indicate that brand image and quality products act as a buffer against Sales Promotionon on purchasing decisions for UMKM products in Palembang.

Keywords: Brand Image, Brand Quality Product, Sales Promotion, Product Purchase Decision, UMKM

Abstrak
Upaya peningkatan penjualan produk UMKM dibutukan strategi baru tentang pemasaran, penjualan dan model marketing yang mampu mempertahankan pertumbuhan dan perkembangan usaha kecil. Penelitian ini bertujuan untuk menganalisis bukti empiris peran brand image, brand quality product sebagai pemediasi dari pengaruh Sales Promotionterhadap keputusan pembelian produk UMKM di Kota Palembang. Jenis penelitian ini adalah survei dengan pendekatan cross sectional. Populasi dari penelitian konsumen UMKM yang ada di Kota Pelembang. Sampel yang diperoleh sebanyak 220 responden, teknik pengambilan sampel meggunakan simple random sampling. Instrumen penelitian yang digunakan berupa angket. Teknik analisis data yang digunakan adalah Structural Equation Modeling (SEM). Hasil Penelitian menujukkan bahwa brand image dan brand quality product berperan sebagai pemediasi dari pengaruh Sales Promotionterhadap keputusan pembelian produk UMKM di Kota Palembang.

Kata kunci: Citra Produk, Citra Kualitas Produk, Promosi Penjualan, Keputusan Membeli Produk, UMKM
Analisis Perbandingan Kinerja Keuangan dan Kinerja Pasar Sebelum dan Sesudah Akuisisi pada Perusahaan Pengakuisisi (Studi Kasus pada Perusahaan Publik yang Melakukan Akuisisi pada Tahun 2019-2020)
Authors: Alandra Kishi , Yunita Irni 
Published: November 2022

Competition between companies in this era of globalization requires the right strategy to realize the desired company goals, and one of the strategies is acquisition. Acquisition activities in Indonesia in 2019-2020 have increased because of the types of transactions and the scope of transactions. Acquisition activities carried out will be reflected in the company’s performance, namely financial and market performance. This study aims to determine the company's financial performance and market performance before and after the acquisition. This study uses a different test analysis technique: the Wilcoxon Signed Rank Test and the Paired T-Test. The results of this study are that the financial performance described by CR, DER, TATO, NPM, ROA, EPS and PER there is no significant difference before and after the acquisition and market performance as described by Tobin’s Q there is no significant difference before and after the acquisition.
Keywords: acquisition; financial performances; market performances

Abstrak
Persaingan antar perusahaan pada era globalisasi ini diperlukan strategi yang tepat untuk guna memenuhi tujuan yang diinginkan perusahaan, yaitu akuisisi. Kegiatan akuisisi di Indonesia pada tahun 2019-2020 mengalami peningkatan karena jenis transaksi dan ruang lingkup transaksi mengalami peningkatan. Kegiatan akuisisi yang dilakukan akan tercermin pada kondisi kinerja keuangan dan kinerja pasar. Penelitian bertujuan untuk mengetahui apakah terdapat perbedaan kinerja keuangan dan kinerja pasar perusahaan sebelum dan sesudah akuisisi. Penelitian ini menggunakan teknik analisis data dengan Uji Wilcoxon Signed Rank Test serta Uji Paired T-Test. Hasil penelitian bahwa kinerja keuangan yang digambarkan dengan CR, DER, TATO, NPM, ROA, EPS dan PER tidak ada perbedaan yang signifikan sebelum dan sesudah akuisisi serta kinerja pasar yang digambarkan dengan Tobin’s Q tidak ada perbedaan yang signifikan sebelum dan sesudah akuisisi.
Kata kunci: akuisisi; kinerja keuangan; kinerja pasar
Karakteristik Manajemen Sebagai Determinan Kreativitas Karyawan yang di Mediasi Kepuasan Kerja Karyawan:
Authors: Sofyan Joel Faruk , Putri Rizki Amelia , Purnama Suryari 
Published: November 2022

The barrier separating employees and their co-workers or employees from their supervisors plays an essential role in maintaining an organization is going concerned because it affects employee job satisfaction and employee creativity. Although employee job satisfaction has been studied extensively, the intervening role of employee job satisfaction in the relationship between management characteristics and employee creativity is yet sparsely been researched. The present study analyses the relationships between supervisor humility and abusive supervision and employee creativity mediated by employee job satisfaction. This study was conducted using an online survey disseminated in May – June 2022, where 180 employees of the Ministry of Maritime Affairs and Fisheries located in Jakarta were selected with a purposive sampling method. The Structural Equation Model was utilized as an inferential analysis tool. The results of this study indicated that supervisor humility has a positive effect on employee job satisfaction.In contrast, supervision affects employee job satisfaction negatively. Third, employee job satisfaction has a positive impact on employee creativity. Lastly, employee job satisfaction mediates the relationship between the supervisor's humility and abusive supervision toward employee creativity.

Keywords: Abusive Supervisor, Employee Creativity, Employee Job Satisfaction, Supervisor Humility.

Abstrak
Hambatan yang memisahkan karyawan dengan rekan kerja atau karyawan dengan atasannya memegang peranan penting dalam menjaga kelangsungan hidup suatu organisasi karena mempengaruhi kepuasan kerja karyawan dan kreativitas karyawan. Meskipun kepuasan kerja karyawan telah dipelajari secara ekstensif, peran intervensi kepuasan kerja karyawan dalam hubungan antara karakteristik manajemen dan kreativitas karyawan belum banyak diteliti. Tujuan dari penelitian ini adalah untuk menganalisis hubungan antara kerendahan hati penyelia dan pengawasan yang kasar dan kreativitas karyawan yang dimediasi oleh kepuasan kerja karyawan. Penelitian ini dilakukan dengan menggunakan survei online yang disebar pada bulan Mei – Juni 2022 dimana 180 pegawai Kementerian Kelautan dan Perikanan yang berada di Jakarta dipilih dengan metode Purposive Sampling dan Structural Equation Model digunakan sebagai alat analisa inferensial. Hasil penelitian ini menunjukkan bahwa kerendahan hati penyelia berpengaruh positif terhadap kepuasan kerja karyawan. Kedua, pengawasan yang kasar berpengaruh negatif terhadap kepuasan kerja karyawan. Ketiga, kepuasan kerja karyawan berpengaruh positif terhadap kreativitas karyawan. Terakhir, kepuasan kerja karyawan memediasi hubungan antara kerendahan hati penyelia dan pengawasan yang kasar terhadap kreativitas karyawan.

Kata kunci: Kepuasan Kerja Karyawan, Kerendahan Hati Penyelia, Kreativitas Karyawan, Pengawasan yang Kasar.
Peran Pemerintah dan Partisipasi Masyarakat dalam Peningkatan Usaha Mikro, Kecil, dan Menengah (UMKM) pada Masa Pandemi COVID-19 di Cipageran, Cimahi
Authors: Tumija Tumija 
Published: November 2022

Usaha Mikro Kecil dan Menengah (UMKM) merupakan kegiatan yang menunjang perekonomian nasional. Namun semenjak COVID-19 melanda di Indonesia, usaha pada UMKM di Kota Cimahi yang terkena dampaknya rata-rata 40%. Tujuan kegiatan pengabdian kepada masyarakat berbasis riset dan kajian ini mengetahui peran pemerintah kota Cimahi dan partisipasi masyarakat dalam meningkatkan usaha UMKM. Metode yang digunakan dalam kegiatan pengabdian kepada masyarakat berbasis riset dan kajian ini adalah Participatory Action Research (PAR). Metode ini dilakukan setelah penulis memaparkan materi, dilanjutkan dengan partisipasi masyarakat berupa diskusi dan sharing. Hasil pembahasan kegiatan pengabdian masyarakat di Kota Cimahi bahwa peran pemerintah Kota Cimahi sudah baik sesuai dengan teori Gede Diva dan PP No. 7 Tahun 2021. Hal ini terlihat dari beberapa fasilitasi baik dari sarana dan prasarana seperti Gedung Technopark sebagai tempat pelatihan UMKM, kemudian fasilitasi pelatihan digital marketing, pelatihan SDM, pelatihan pengembangan produk. Selanjutnya peran masyarakat (pelaku UMKM) sudah aktif dalam mengikuti rapat-rapat yang diselenggarakan oleh dinas terkait dalam perencanaan program kegiatan, dan aktif dalam kegiatan-kegiatan kegiatan serta telah memanfaatkan hasil kegiatan. Dari hasil pembahasan tersebut dapat disimpulkan bahwa pemerintah Kota Cimahi telah melaksanakan tiga peran sebagai fasilitator, regulator dan katalisator dan masyarakat sudah berpartisipasi aktif dalam perencanaan, pelaksanaan dan pemanfaatan hasil dalam meningkatkan UMKM.
Sosialisasi dan Pendampingan Pembuatan Nomor Induk Berusaha (NIB) melalui Online Single Submission (OSS) kepada Pelaku Usaha Mikro di Desa Sukahayu Kecamatan Rancakalong Kabupaten Sumedang
Authors: Marthalina Marthalina , Khairina Utami 
Published: November 2022

Pandemi COVID-19 telah berdampak terhadap melemahnya perekonomian masyarakat Desa Sukahayu yang ditandai dengan meningkatnya angka pengangguran. Banyak masyarakat yang mencoba peruntungan dengan membuka usaha baru yang secara administrasi masih belum memiliki izin usaha. Dari total sekitar 400 UMKM di Desa Sukahayu hanya 179 UMKM yang telah mendaftarkan izin usaha karena sebagian besar pelaku usaha menjalankan aktivitas jual beli di hari kerja, belum mampu menggunakan teknologi internet, serta belum mendapat informasi dan mengetahui urgensi pembuatan izin usaha. Sebagai bentuk upaya mengatasi permasalahan tertib administrasi di Kantor Desa Sukahayu, tim pengabdian masyarakat telah melaksanakan sosialisasi dan pendampingan secara langsung pembuatan nomor induk berusaha (NIB) melalui online single submission (OSS) di Kantor Desa Sukahayu dan secara door to door ke rumah masyarakat yang memiliki usaha mikro. Tujuan kegiatan pengabdian masyarakat ini adalah memberikan pemahaman tentang pentingnya NIB dan OSS digital untuk mendapatkan kemudahan legalitas usaha serta dokumen lainnya seperti NPWP badan atau perorangan. Kegiatan pengabdian masyarakat dilakukan dengan metode sosialisasi dan pendampingan kepada masyarakat yang dilaksanakan selama kurang lebih satu bulan serta memiliki sasaran khusus bagi masyarakat yang belum memiliki nomor induk berusaha (NIB) tetapi sudah memiliki atau menjalankan usaha mikro. Kegiatan pengabdian masyarakat ini telah berkoordinasi langsung kepada pihak pemerintahan Desa Sukahayu. Hasil dari kegiatan pengabdian masyarakat ini adalah diterbitkannya 32 nomor induk berusaha (NIB), bertambahnya pengetahuan dan informasi beberapa masyarakat tentang pentingnya nomor induk berusaha bagi para pelaku usaha mikro di Desa Sukahayu serta meningkatnya keinginan masyarakat untuk mendaftarkan usahanya secara legal yang ditandai dengan masuknya dokumen usulan baru sebanyak 17 buah. Saran masukan untuk optimalisasi pembuatan NIB di Desa Sukahayu adalah dibutuhkan komitmen dan konsistensi pemerintah Desa Sukahayu untuk melanjutkan kegiatan sosialisasi, pendampingan door to door dan pendampingan yang lebih serius bagi pelaku usaha mikro karena masih ada kurang lebih 200 lebih usaha mikro yang belum terdaftar melalui OSS dan masih banyak masyarakat yang belum memahami pentingnya nomor induk berusaha bagi para pelaku usaha yang disebabkan asumsi masyarakat yang menganggap untuk mengurus izin dan legalitas usaha membutuhkan waktu yang panjang dan merupakan hal yang rumit. Kondisi ini sangat perlu diperhatikan dan ditindaklanjuti oleh pemerintah Desa Sukahayu dengan bekerja sama dengan Dinas terkait.
Oral microbiota and periodontitis severity among Hispanic adults
Authors: Ortiz Ana P. , Acosta-Pagán Kimil T. , Oramas-Sepúlveda Carla , Castañeda-Avila Maira A. , Vilanova-Cuevas Brayan , Ramos-Cartagena Jeslie M. , Vivaldi José A. , Pérez-Santiago Josué , Pérez Cynthia M. , Godoy-Vitorino Filipa 
Published: November 2022

BackgroundPeriodontitis, one of the most common bacterial infections characterized by chronic inflammation, is also known to be a risk factor for chronic conditions, including cardiovascular disease and cancer. This inflammation is driven by an altered microbiota with an increase in pathogenic bacteria. We evaluated the association between oral microbiota and periodontitis severity in high-risk Hispanics.MethodThis cross-sectional study recruited 134 sexually active participants aged 21 to 49 years old from STI Clinics in Puerto Rico. A periodontal examination, saliva collection, and an interviewer-administered questionnaire were performed. Periodontal severity was categorized as: having no disease, mild, and moderate/severe and BOP and tooth loos was noted. Saliva samples were collected for genomic DNA extraction, downstream 16S rDNA amplification sequencing, and bioinformatics analyses.ResultsThe structure, composition, and diversity of bacterial communities differed significantly according to periodontal severity. The richness and overall diversity also differed between participants without periodontitis and participants with some level of periodontal disease. A higher abundance of Prevotella, Veillonella, or Treponema was attributed to periodontal disease and Aggregatibacter to severe bleeding on probing, while Neisseria was found in higher abundance in healthy participants, decreasing its levels with drinking, smoking, and oral sex practices.ConclusionsOur findings indicate that dysbiosis occurs as periodontal disease progresses, and both alcohol consumption and smoking habits pose risk factors for oral dysbiosis. These results are of public health and clinical impact, as several bacteria identified could serve in the future as biomarkers for periodontitis and oral cancer risk.
Decision-centered design of a clinical decision support system for acute management of pediatric congenital heart disease
Authors: Assadi Azadeh , Laussen Peter C. , Freire Gabrielle , Ghassemi Marzyeh , Trbovich Patricia 
Published: November 2022

Background and ObjectivesChildren with congenital heart disease (CHD), have fragile hemodynamics and can deteriorate due to common childhood illnesses and the natural progression of their disease. During these acute periods of deterioration, these children often present to their local emergency departments (ED) where expertise in CHD is limited, and appropriate intervention is crucial to their survival. Previous studies identified that determining the appropriate intervention for CHD patients can be difficult for ED physicians, particularly since key components of effective decision making are not being met. Although key components of effective decision making for ED physicians have been identified, they have yet to be transformed into actionable guidance. We used decision centered design (DCD) to translate key components of decision making into decision requirements and associated design concepts, that we subsequently incorporated into a prototype clinical decision support system (CDSS).MethodsUsing framework analysis, transcripts from Critical Decision Method interviews of CHD experts and ED physicians were inductively coded to identify key decision requirements for ED physicians that are currently not well supported, and their associated design concepts. A design workshop was held to refine the identified key decision requirements and design concepts as well as to sketch information that would satisfy the identified requirements. These were iteratively incorporated into a prototype CDSS.ResultsThree decision requirements: (1) distinguish the patient's unique physiology based on their unique cardiac anatomy, (2) explicitly consider CHD specific differential diagnoses to allow a more structured reflection of diagnosis, and (3) select CHD appropriate interventions for each patient, were identified. These requirements along with design concepts and information needs identified through the design workshop were incorporated into the CDSS prototype.ConclusionWe identified key decision requirements and associated design concepts, that informed the design of a CDSS to provide actionable guidance for ED physicians when managing CHD patients. Meeting ED physicians' decision components with a CDSS requires the translation of their key decision requirements in its design. If not, we risk creating designs that interfere with clinician performance.
Tandem mass tag-based proteomic profiling revealed potential therapeutic targets and mechanisms of liraglutide for the treatment of impaired glucose tolerance
Authors: Guo Qiuyue , Han Cong , Xu Yunsheng , Chen Qingguang , Han Xu , Zhao Sen , Li Jie , Lu Hao 
Published: November 2022

ObjectiveBased on the tandem mass tag (TMT) technique, our study investigated the potential therapeutic targets of Liraglutide (LIRA) on streptozotocin (STZ) induced impaired glucose tolerance (IGT) in rats and discuss the biological mechanism of the drug against IGT.Methods10 rats were randomly selected from 31 male wistar rats of specific pathogen free (SPF) grade as control group and fed with conventional chow, offered the remaining rats a high fat and high sugar (HFSD) diet combined with an intraperitoneal injection of STZ to establish the IGT model, and excluded 2 non-model rats. Specifically, the model rats were randomly divided into Model group (n=10) and LIRA group (n=9). In addition, the LIRA group was subcutaneously injected with 0.06 mg/kg LIRA, during which the metabolic parameters including body weight and fasting blood glucose were recorded. After 8 weeks, samples were taken under anesthesia. Then, the cell morphology was observed using HE staining, and immunofluorescence was performed on the pancreatic tissues of the three groups of rats. Besides, the expression of differential proteins in pancreatic tissues of the three groups of rats was determined by the TMT proteomic labeling. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological function analysis were performed on the intersection of Model and LIRA differential proteins.ResultsLIRA could not only significantly reduce blood glucose levels but also improve islet cell morphology and function in IGT rats. Among the differential proteins between the model group and the blank group, 44 were reversed after LIRA treatment, of which 14 were up-regulated, while 30 were down-regulated, including PPIF, MPRIP, CYP51, TXNL1, BCL-2, etc. (FC&gt;1.1 or&lt;0.909, P&lt;0.05). According to the GO and KEGG analysis results, it was related to biological processes such as fatty acid metabolism and adipocyte generation, which involved multiple signaling pathways regulating the function of islet cells, such as MAPK, PI, Ras, FcγR, and unsaturated fatty acids, and pyruvate metabolism.ConclusionTo sum up, LIRA participated in anti-IGT therapy through regulation of multiple target proteins and biological functions. This study is of great reference for further exploring the mechanism of action of LIRA at the protein level of IGT.
Cocultivation of Chinese prescription and intestine microbiota: SJZD alleviated the major symptoms of IBS-D subjects by tuning neurotransmitter metabolism
Authors: Xia Xiuwen , Xie Ya , Chen Qiaoqiao , Ding Dou , Wang Zongqin , Xu Yaji , Wang Yili , Wang Xiumin , Ding Weijun 
Published: November 2022

ObjectiveDiarrhea-predominant irritable bowel syndrome (IBS-D) is a recurrent and common disease featuring dysbiotic intestinal microbiota, with limited treatments. Si-Jun-Zi Decoction (SJZD), a classic Chinese prescription, has been extensively used for IBS-D. This work aimed to explore the ex vivo interactions of SJZD and IBS-D’s intestinal microbiota.MethodsFive samples of intestinal microbiota collected from IBS-D volunteers and five age-matched healthy controls were recruited from the Affiliated Hospital, Chengdu University of Traditional Chinese Medicine (TCM). A representative mixture of intestinal microbiota was composed of an equal proportion of these fecal samples. To simulate the clinical interaction, this microbiota was cocultivated with SJZD at clinical dosage in an anaerobic incubator at 37°C for 35 h. Microbiota and metabolic alterations were assessed by 16S rRNA gene sequencing in the V3/V4 regions and a nontargeted metabolome platform, respectively.ResultsAfter being cocultivated with SJZD, the dysbiotic intestine microbiota from IBS-D subjects was largely restored to those of the healthy controls. A total of 624 differentially expressed metabolites were detected by nontargeted metabolomics, of which 16 biomarkers were identified. These metabolites were then enriched into 11 pathways by KEGG, particularly those involved in neurotransmitter metabolism responses for the major symptom of IBS-D. Correlation analysis of bacterial metabolites demonstrated a synergistic pattern of neurotransmitter metabolism between Streptococcus and E. Shigella.ConclusionSJZD rescued the dysbiotic intestinal microbiota and ameliorated the dysfunctional neurotransmitter metabolism involved in IBS-D’s major symptoms.