bluuebunny/crossref_metadata_2025_split huggingface dataset:
The use of various appointment systems among patients visiting academic outpatient centers in Kerman and the evaluation of patients’ perspective and satisfaction
Authors: Bagheri Fatemeh , Behnam Farzaneh , Galavi Zahra , Ahmadian Leila 
Published: November 2022

Abstract
Background
To optimize appointment systems, it is necessary to assess their users’ perspectives. This study aims to determine the use of various appointment systems among patients in academic outpatient centers and to investigate their perspectives and satisfaction.

Methods
This survey study was conducted on 332 patients or those accompanying patients in academic outpatient centers. A five-part questionnaire consisting of (1) demographic information, (2) willingness to use systems, (3) problems when using these systems, (4) problems after reserving the appointment, (5) recommendations and critics was used. The relationship between the system of interest and the available tools was examined by the Chi-square test, and the relationship between demographic characteristics and satisfaction was assessed using multiple regression.

Results
The participants’ overall satisfaction towards appointment systems, regardless of the type of system, was 49.12 ± 16.04 (out of 100). Satisfaction with the appointment system using Unstructured Supplementary Service Data (USSD) was significantly higher than the other two systems (p = 0.03). Web-based application and Interactive Voice Response (IVR) were the most frequently used systems with 61% and 48%, respectively. More than half of those who had access to a telephone (56%) preferred the IVR appointment system, and most of those who had Internet access (71%) preferred the web-based application (p < 0.05). Among 137 participants who had access to both the Internet and telephone, 49% (n = 67) stated that they would rather arrange their appointment through the web-based application.

Conclusion
The web-based application and IVR are the most frequently used and favorable appointment system among the patients or those accompanying patients. Despite the availability of the infrastructure, the participant had moderate satisfaction with these systems due to their failures. Therefore, to have more efficient systems and increase patients or those accompanying patients satisfaction with these systems, healthcare authorities should have a plan to solve the problems of these systemes and use the capacity of information resources to inform the community regarding these systems.
Universal testing for hepatitis B and hepatitis C in the emergency department: a cost-effectiveness and budget impact analysis of two urban hospitals in the United Kingdom
Authors: Williams Jack , Vickerman Peter , Smout Elizabeth , Page Emma E. , Phyu Khine , Aldersley Mark , Nebbia Gaia , Douthwaite Sam , Hunter Laura , Ruf Murad , Miners Alec 
Published: November 2022

Abstract
Background
Numerous studies have shown the effectiveness of testing for hepatitis B (HBV) and hepatitis C (HCV) in emergency departments (ED), due to the elevated prevalence amongst attendees. The aim of this study was to conduct a cost-effectiveness analysis of universal opt-out HBV and HCV testing in EDs based on 2 long-term studies of the real-world effectiveness of testing in 2 large ED’s in the UK.

Methods
A Markov model was used to evaluate ED-based HBV and HCV testing versus no ED testing, in addition to current testing practice. The two EDs had a HBV HBsAg prevalence of 0.5–0.9% and an HCV RNA prevalence of 0.9–1.0%. The analysis was performed from a UK health service perspective, over a lifetime time horizon. Costs are reported in British pounds (GBP), and outcomes as quality adjusted life years (QALYs), with both discounted at 3.5% per year. Incremental cost-effectiveness ratios (ICER) are calculated as costs per QALY gained. A willingness-to-pay threshold of £20,000/QALY was used. The cost-effectiveness was estimated for both infections, in both ED’s.

Results
HBV and HCV testing were highly cost-effective in both settings, with ICERs ranging from £7,177 to £12,387 per QALY gained. In probabilistic analyses, HBV testing was 89–94% likely to be cost-effective at the threshold, while HCV testing was 94–100% likely to be cost-effective, across both settings. In deterministic sensitivity analyses, testing remained cost-effective in both locations at ≥ 0.25% HBsAg prevalence, and ≥ 0.49% HCV RNA prevalence. This is much lower than the prevalence observed in the two EDs included in this study.

Conclusions
HBV and HCV testing in urban EDs is highly cost-effective in the UK, and can be cost-effective at relatively low prevalence. These results should be reflected in UK and European hepatitis testing guidelines.
The ERα-NRF2 signalling axis promotes bicalutamide resistance in prostate cancer
Authors: Tian Lei , Peng Yanfei , Yang Kuo , Cao Jiasong , Du Xiaoling , Liang Zhixian , Shi Jiandang , Zhang Ju 
Published: November 2022

Abstract
Background
Bicalutamide is a nonsteroidal antiandrogen widely used as a first-line clinical treatment for advanced prostate cancer (PCa). Although patients initially show effective responses to bicalutamide treatment, resistance to bicalutamide frequently occurs and leads to the development of castration-resistant PCa (CRPC). This research investigated the roles of the oestrogen receptor α (ERα)-nuclear factor E2-related factor 2 (NRF2) signalling pathway in bicalutamide resistance in PCa cells.

Methods
We performed bioinformatic analysis and immunohistochemical staining on normal and cancerous prostate tissue to evaluate ERα and NRF2 expression and their correlation. Gene expression and localization in PCa cell lines were further investigated using real-time reverse transcription PCR/Western blotting and immunofluorescence staining. We treated PCa cells with the ER inhibitor tamoxifen and performed luciferase reporter assays and chromatin immunoprecipitation (ChIP) assays to understand ERα-dependent NRF2 expression. Overexpression and knockdown of ERα and NRF2 were used to explore the potential role of the ERα-NRF2 signalling axis in bicalutamide resistance in PCa cells.

Results
We found that the expression of ERα and NRF2 was positively correlated and was higher in human CRPC tissues than in primary PCa tissues. Treatment with oestrogen or bicalutamide increased the expression of ERα and NRF2 as well as NRF2 target genes in PCa cell lines. These effects were blocked by pretreatment with tamoxifen. ChIP assays demonstrated that ERα directly binds to the oestrogen response element (ERE) in the NRF2 promoter. This binding led to increased transcriptional activity of NRF2 in a luciferase reporter assay. Activation of the ERα-NRF2 signalling axis increased the expression of bicalutamide resistance-related genes. Inhibition of this signalling axis by knockdown of ERα or NRF2 downregulated the expression of bicalutamide resistance-related genes and inhibited the proliferation and migration of PCa cells.

Conclusions
We demonstrated the transcriptional interaction between ERα and NRF2 in CRPC tissues and cell lines by showing the direct binding of ERα to the ERE in the NRF2 promoter under oestrogen treatment. Activation of the ERα-NRF2 signalling axis contributes to bicalutamide resistance in PCa cells, suggesting that the ERα-NRF2 signalling axis is a potential therapeutic target for CRPC.
Use of social network as a coping strategy for depression among young people during the COVID-19 lockdown: findings from the COMET collaborative study
Authors: Orsolini Laura , Volpe Umberto , Albert Umberto , Carmassi Claudia , Carrà Giuseppe , Cirulli Francesca , Dell’Osso Bernardo , Del Vecchio Valeria , Di Nicola Marco , Giallonardo Vincenzo , Luciano Mario , Menculini Giulia , Nanni Maria Giulia , Pompili Maurizio , Sani Gabriele , Sampogna Gaia , Tortorella Alfonso , Fiorillo Andrea 
Published: November 2022

Abstract
Background
Use of social media (SM) has exponentially grown particularly among youths in the past two years, due to COVID-19-related changing lifestyles. Based on the Italian COvid Mental hEalth Trial (COMET), we investigated the association between SM use and depressive symptoms among Italian young adults (aged 18–24).

Methods
The COMET is a nationwide multi-center cross-sectional study that investigated socio-demographic data, social networking addiction (BSNAS), depression, anxiety, and stress (DASS-21), as well as impulsiveness (BIS-15) and aggressiveness (AQ) in a large sample of youngsters, in order to assess the association between BSNAS and DASS-21 indices. Mediation analyses were performed to evaluate the role of impulsiveness and aggressive personality traits in the association between SM use (SMU) and depression.

Results
75.8% of the sample (n = 491) had a problematic SMU. SMU was reduced by high AQ and high DASS-21 scores (F = 42.338, p < 0.001, R2 = 0.207). Mediation analyses showed that SMU negatively predicted depressive symptomatology with the interaction mediated by AQ total (ß = − 0.1075), physical (ß = − 0.207) and anger (ß = − 0.0582), BIS-15 total (ß = − 0.0272) and attentional (ß = − 0.0302). High depressive levels were predicted by high AQ scores, low SMU levels, low verbal and physical AQ, and low attentional BIS-15 (F = 30.322, p < 0.001, R2 = 0.273). Depressive symptomatology negatively predicted SMU with their interaction mediated by AQ total (ß = − 0.1640), verbal (ß = 0.0436) and anger (ß = − 0.0807), BIS-15 total (ß = − 0.0448) and attentional (ß = − 0.0409).

Conclusions
SMU during the early phases of the COVID-19 pandemic could have a beneficial role in buffering negative consequences linked to social isolation due to quarantine measures, despite this association being mediated by specific personality traits.
Alcohol use during COVID-19 pandemic on the long run: findings from a longitudinal study in Germany
Authors: Schecke Henrike , Bohn Annette , Scherbaum Norbert , Mette Christian 
Published: November 2022

Abstract
Background
The impact of COVID-induced stress on mental health and alcohol use has been demonstrated in recent research. However, there is a lack of longitudinal data since most studies reported on cross-sectional data. It remains unclear how alcohol use develops under the dynamic changes of the pandemic. Therefore, the present study aims to investigate the general development of alcohol use and the impact of COVID related stress on drinking behavior in a German population-based sample during the pandemic in 2020.

Methods
In the longitudinal design with three measurements (baseline [T1] and two follow-ups [T2, T3]) an online survey was administered. The survey included the Alcohol Use Disorder Identification Test (AUDIT) as well as the assessment of the drinking days in the last 30 days, the number of alcoholic beverages on each occasion and changes in alcohol use in the previous fourteen days. Further, COVID-19 related concerns, perceived stress, worries about friends and family and worries about the financial situation were also assessed and multiple linear regressions and confidence intervals (CI) were calculated.

Results
1050 participants started the survey, 756 participants (71.4%), 317 (52.7%) participants completed the survey at all three measurements. Seventy six percent (n = 241) of the sample were female. An increase in alcohol use in the previous 14 days was reported by 10.9% at T1, 3.9% at T2 and 3.6% at T3. Moreover, a decrease in alcohol use in the previous 14 days was reported by 8.7% at T1, 6.5% at T2 and 4.1% at T3. The number of drinking days was significantly higher at T2 than at baseline (p < .001; F = − 2.06, [CI − 3.10, − 1.02]). COVID-19 related concerns and stress were associated with a higher number of drinking days and average number of drinks at a typical occasion. AUDIT score at baseline positively predicted number of drinking days as well as average number of drinks.

Conclusions
The significant increases in alcohol use is a public health issue during COVID-19 pandemic. The findings show that especially people who drink more hazardously previously tend to drink more under pandemic conditions. Those individuals are particularly at risk for developing substance-related problems.
A systematic review of the prediction of consumer preference using EEG measures and machine-learning in neuromarketing research
Authors: Byrne Adam , Bonfiglio Emma , Rigby Colin , Edelstyn Nicky 
Published: November 2022

Abstract
Introduction
The present paper discusses the findings of a systematic review of EEG measures in neuromarketing, identifying which EEG measures are the most robust predictor of customer preference in neuromarketing. The review investigated which TF effect (e.g., theta-band power), and ERP component (e.g., N400) was most consistently reflective of self-reported preference. Machine-learning prediction also investigated, along with the use of EEG when combined with physiological measures such as eye-tracking.

Methods
Search terms ‘neuromarketing’ and ‘consumer neuroscience’ identified papers that used EEG measures. Publications were excluded if they were primarily written in a language other than English or were not published as journal articles (e.g., book chapters). 174 papers were included in the present review.

Results
Frontal alpha asymmetry (FAA) was the most reliable TF signal of preference and was able to differentiate positive from negative consumer responses. Similarly, the late positive potential (LPP) was the most reliable ERP component, reflecting conscious emotional evaluation of products and advertising. However, there was limited consistency across papers, with each measure showing mixed results when related to preference and purchase behaviour.

Conclusions and implications
FAA and the LPP were the most consistent markers of emotional responses to marketing stimuli, consumer preference and purchase intention. Predictive accuracy of FAA and the LPP was greatly improved through the use of machine-learning prediction, especially when combined with eye-tracking or facial expression analyses.
Formulation mitigations for particle formation induced by enzymatic hydrolysis of polysorbate 20 in protein-based drug products: insights from a full-factorial longitudinal study
Authors: Yuk Inn H. , Koulis Theo , Doshi Nidhi , Gregoritza Kathrin , Hediger Constanze , Lebouc-Haefliger Vanessa , Giddings Jamie , Khan Tarik A. 
Published: November 2022

Abstract
Hydrolytic degradation of the polysorbate 20 (PS20) surfactant in protein-based liquid formulations releases free fatty acids (FFAs), which can accumulate to form particles in drug products during real-time (long-term) storage. To identify formulation conditions that mitigate the risk of particle formation, we conducted a longitudinal study using purified recombinant monoclonal antibody (mAb) formulated in 24 conditions. In this real-time stability study at 5 °C, three key formulation parameters—mAb concentration, initial PS20 concentration, and pH—were varied across representative ranges in a full-factorial design. A longitudinal regression analysis was used to evaluate the effects of these parameters and their interactions on PS20 degradation (via measurements of PS20, FFAs, and PS20 ester distribution) and on particle formation (via visible particle observations and subvisible particle counts). The time-dependent onset of visible particles trended with the rise in subvisible particle counts and FFA levels and fall in PS20 concentration. In the ranges studied here, lower mAb concentration and higher initial PS20 concentration delayed the onset of particles, whereas pH had a negligible effect. These observations were consistent with the general trends predicted by our previously published FFA solubility model. Taken together, these findings highlight the complex relationships between formulation parameters, PS20 degradation, and particle formation.
Correlation of parasite density and biochemical parameters in children with malaria infection in Calabar, South-South Nigeria
Authors: Okon Akpan Uwem , Eze Bassey Iya , Emmanuel Uwaetteh Akanimoh , Marcus Inyang Wisdom , Adanna Usoro Chinyere 
Published: November 2022

Abstract
Background
Malaria parasitemia is associated with premature, excessive hemolysis, induction of oxidative stress, and derangement in metabolism of iron, proteins, and some electrolytes. This study aims to evaluate the effects of malaria infection on ascorbic acid (AA), uric acid (UA), iron, transferrin, albumin, total iron-binding capacity (TIBC), calcium, and magnesium levels in malaria infection.

Results
Among the 50 malaria-infected children, 12 had severe parasitemia (PD ≥ 10,000 parasites/μl), 16 had moderate parasitemia (PD: 2000 to < 10,000 parasites/μl), and 22 children had mild parasitemia (PD < 2000 parasites/μl). The AA, iron, transferrin, and TIBC levels were significantly lower and UA and Mg higher in malaria-infected children compared with the controls. Ascorbic acid correlated negatively with UA and PD in malaria-infected children. Iron correlated positively with albumin, transferrin, and TIBC in malaria-infected children, while Ca correlated negatively with Mg levels.

Conclusion
Malaria infection in children is associated with reduced AA and iron parameters and increased UA and Mg levels; hence, vitamin C and iron supplementation could be useful in malaria therapy.
A century of Azolla filiculoides biocontrol: the economic value of Stenopelmus rufinasus to Great Britain
Authors: Pratt Corin F. , Constantine Kate , Wood Suzy V. 
Published: November 2022

Abstract
Background
The invasive aquatic fern Azolla filiculoides has been present in Great Britain (GB) since the end of the nineteenth century, while its specialist natural enemy, the weevil Stenopelmus rufinasus was first recorded nearly four decades later, in 1921. The purpose of this study was to estimate the economic value of management cost savings resulting from the presence of S. rufinasus as a biocontrol agent of A. filiculoides in GB, including the value of additional augmentative releases of the weevil made since the mid-2000s, compared with the expected costs of control in the absence of S. rufinasus.

Methods
Estimated economic costs (based on the length/area of affected waterbodies, their infestation rates, and the proportion targeted for management) were calculated for three scenarios in which A. filiculoides occurs in GB: (1) without weevils; (2) with naturalised weevil populations; and (3) with naturalised weevil populations plus augmentative weevil releases.

Results
In the absence of biocontrol, the expected average annual costs of A. filiculoides management were estimated to range from £8.4 to 16.9 million (US$9.4 to 18.9 million) (£1 = US$1.12). The impacts of naturalised S. rufinasus populations on A. filiculoides were expected to reduce management costs to £0.8 to 1.6 million (US$0.9 to 1.8 million) per year. With additional augmentative releases of the weevil, A. filiculoides management costs were estimated to be lower still, ranging from £31.5 to 45.8 thousand (US$35.3 to 51.3 thousand) per year, giving an estimated benefit to cost ratio of augmentative S. rufinasus releases of 43.7:1 to 88.4:1.

Conclusions
The unintentional introduction of the weevil S. rufinasus to GB is estimated to have resulted in millions of pounds of savings annually in management costs for A. filiculoides. Additional augmentative releases of the weevil provide further net cost savings, tackling A. filiculoides outbreaks and bolstering naturalised populations. The use of herbicides in the aquatic environment is likely greatly reduced due to A. filiculoides biocontrol. Although somewhat climate-limited at present in GB, climate change may result in even more effective biocontrol of A. filiculoides by S. rufinasus as has been observed in warmer regions such as South Africa, where the plant is no longer considered a threat since the introduction of the weevil.
Four-compartment diffusion model of cortisol disposition: Comparison to three alternative models in current clinical use
Authors: Dorin Richard I , Urban Frank K , Perogamvros Ilias , Qualls Clifford R 
Published: November 2022

ABSTRACT

Context
Estimated rates of cortisol elimination and appearance vary according to the model used to obtain them. Generalizability of current models of cortisol disposition in healthy humans is limited.

Objective
Development and validation of a realistic, mechanistic model of cortisol disposition that accounts for the major factors influencing plasma cortisol concentrations in vivo (Model 4), and comparison to previously described models of cortisol disposition in current clinical use (Models 1-3).

Methods
The four models were independently applied to cortisol concentration data obtained for the hydrocortisone bolus experiment (20 mg) in two clinical groups: healthy volunteers (HV, n = 6) and CBG-deficient (n = 2). Model 4 used Fick’s first law of diffusion to model free cortisol flux between vascular and extravascular compartments. Pharmacokinetic parameter solutions for Models 1-4 were optimized by numerical methods, and model-specific parameter solutions were compared by RM-ANOVA. Models and respective parameter solutions were compared by mathematical and simulation analyses, and an assessment tool was used to compare performance characteristics.

Results
Cortisol half-lives differed significantly between models (all P &lt; 0.001) with significant model-group interaction (P = 0.02). In comparative analysis, Model 4 solutions yielded significantly reduced free cortisol half-life, improved fit to experimental data (both P &lt; 0.01), and superior model performance.

Conclusions
The proposed 4-compartment diffusion model (Model 4) is consistent with relevant experimental observations and met the greatest number of empiric validation criteria. Cortisol half-life solutions obtained using Model 4 were generalizable between HV and CBG-deficient groups and bolus and continuous modes of hydrocortisone infusion.
A Queer Visitation
Authors: Boyd Brenton 
Published: November 2022

Abstract
This article meditates on black unbelonging as the haunting impasse reached when attempting to wrest a phenomenological account of black life amid perpetual black death. It seeks to expose the calculated dislocation of black modes of being, embodiment, and sensation from hegemonic categories of experience that serve to capture, to surveil, and to leave Blacks in a state of “constitutive discomfort.” Rather than merely signifying non-ontology, alienation, or racialization, black unbelonging further allegorizes self-possession and originary constitution as haunting the interpellative processes that maintain the Western subject of phenomenology in modernity. After reframing Merleau-Pontian flesh not within a redemptive phenomenology but through a Spillersian invocation of the flesh-of-this-world as the proper object of antiblack terror, the article demonstrates how the protagonists of Randall Kenan's A Visitation of Spirits (1989) and Barry Jenkins's Moonlight (2016) stage black unbelonging through various Gothic conventions. Ultimately the article champions alternatives to phenomenology simpliciter that are ephemerally indexable by what it calls “black necromancy.”