bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of MAGEA6 in digestive system tumours
Authors: yang yihan , Wu Xidong , Li Nianshuang , Chen Sihai , Zhang Lin , Ying Mengqi , Yang Sifan , Chan Matthew Tak Vai , Shu Xu , Wu William Ka Kei , Xie Chuan 
Published: August 2022

Abstract
Digestive system tumours are the most common malignancies worldwide, causing huge number of deaths each year. With the increases in morbidity and mortality, it is imperative to identify new therapeutic targets. Melanoma-associated antigen-A6 (MAGEA6) is a member of the cancer/testicular antigen (CTA) family. There are increasing amount of evidence supporting that MAGEA6 plays an important role in the development of solid tumours. However, there has been no comprehensive analysis of MAGEA6 in digestive system tumours. Therefore, our objective was to investigate the expression level, clinical significance and related biological functions of MAGEA6 in digestive system tumours. In this study, we integratively analysed the data from multiple databases of patients with digestive system tumours. Our results showed that MAGEA6 was highly expressed in most digestive system tumours as compared with normal tissues, whereas patients with higher expression of MAGEA6 had a worse prognosis. Based on co-expression analysis, there was a close interaction between MAGEA6 and MAGEA3. Furthermore, differential gene enrichment analysis indicated that MAGEA6 was mainly involved in the formation of extracellular structure, DNA replication, mitosis and the p53 signalling pathway. These results suggest that MAGEA6 is as a prognostic factor with functional importance in digestive system tumours.
Effect of valve opening on starting performance of pump as turbine
Authors: Zhang Yu-Liang , Li Jin-Fu , Zheng You-Qu 
Published: August 2022

Abstract
The study on transient characteristics of pump as turbines during atypical startup has not been deeply explored yet. In order to reveal the transient characteristics of a small centrifugal pump reversing as turbine during startup process in this paper, the transient hydraulic performance experiments are conducted for three steady rotational speed cases. Under the condition of each case, three, five and, three valve opening scenarios are completed to measure the performance. The dimensionless analysis are also employed so as to better reveal transient behavior of the pump as turbine during atypical startup. The results show that the rise rate of each performance parameter is different, wherein the shaft power and rotational speed have the fastest rising rate, followed by the flow rate, and the head rise is the slowest. It is clearly seen that the shock phenomenon in static pressure easily occurs at the outlet of pump as turbine. With the increase of valve opening, the dimensionless flow rate, head and, power coefficient all show the evolution trend of gradually increasing.
Prognostic factors in patients with chronic myeloid leukemia according to competing-risks analysis: a study based on the SEER database
Authors: Li Wanyue , Zhuang Weisheng , Huang Liying , Shang Yaru , Yan Guoqiang , Zhou Yu , Lu Jianliang , Chen Zhuoming , Lyu Jun 
Published: August 2022

Abstract
Background: The classic Cox proportional-hazards model is generally used to derive risk factors affecting patients with chronic myeloid leukemia (CML). However, when competing risk factors are present, the results of Cox analysis need to be revisited.Objective: This study aimed to develop a competing-risks model to assess the factors that influence predictions of patients with CML in an attempt to produce results that are more accurate than those from Cox analyses.Methods: The CML data in the SEER (Surveillance, Epidemiology, and End Results) database that met our requirements during 1975–2019 were analyzed. Univariate analyses in this study were performed using cumulative incidence functions and Gray’s tests, and the multivariate analysis was performed using three models: Fine-Gray, cause-specific, and Cox proportional-hazards models.Results: Of the 8331 included cases, 4827 (57.94%) died (2459 [29.52%] from CML and 2368 [28.42%] from other causes) and 3504 (42.60) survived. Gray’s test indicated that the outcome was significantly affected by year of diagnosis, age, total number of in situ/malignant tumors, type of reporting source, marital status, and primary indicator of malignancy. The results of the multivariate competing-risks analyses suggested that age, year of diagnosis, total number of in situ/malignant tumors, type of reporting source, marital status, and primary indicator of malignancy were independent risk factors for the prognosis of patients with CML (P<0.05). Conclusions: This study has developed a competing-risks analysis model for assessing the risk factors for patients with CML. Our findings may allow for more-accurate formulation of clinical decisions, saving healthcare resources in the current individualized treatment environment for the benefit of the patients.
Isolation, characterization, and antibiotic sensitivity pattern of Gram-negative bacteria causing chronic bacterial prostatitis
Authors: Mehanni Magda M. , Sayed Osama M. , El-Gendy Ahmed O. , Hozayen Walaa G. , Mohamed Sahar I. 
Published: August 2022

Abstract
Background: Bacterial prostatitis (BP) is an infection of the prostate gland, it can be acute (ABP) or chronic (CBP) and if not treated appropriately, it can result in significant morbidity.Results: In this study, expressed prostate secretion from 10 patients that are diagnosed with chronic bacterial prostatitis at Ain-Shams hospital, Egypt are used to isolate the common causative bacteria for CBP. Results of this study indicated that Gram negative bacteria were predominant since 54 Gram-ve bacterial isolates were obtained out of the total 62 isolates. Gram-ve bacterial isolates were grouped according to their oxidase test results and to lactose fermentation capability into three groups. The largest group contained 38 isolates of oxidase negative, lactose fermenters. Five representative isolates of this group were identified biochemically or by 16S rRNA sequence homology to be related to Enterobacter cloacae complex (3 Enterobacter cloacae related strains and 2 Enterobacter kobei related strains). Oxidase positive lactose non-fermentors group contained 9 isolates while oxidase negative lactose non-fermentors group contained 7 isolates. A representative isolate for each of them were identified by 16S rRNA sequence homology to be a strain of Pseudomonas stutzeri and Providencia stuartii respectively. Antibiotic sensitivity pattern of the chosen representative isolates indicated that all of them were sensitive to ciprofloxacin followed by ampicillin while resistance to cefaclor and aztereonam was prevalent. Other tested antibiotics recorded different sensitivity pattern among tested isolates.Conclusions: isolates of Genus Enterobacter sp. included in the E. cloacae complex group represent the most common bacterial pathogen in tested EPS of CBP diagnosed patients, of them Enterobacter cloacae was the most common.
Dynamic hip screws versus cephalocondylic intramedullary nails for unstable extracapsular hip fractures in 2021: A systematic review and meta-analysis of randomised trials
Authors: Raj Siddarth , Grover Sarika , Bola Harroop , Pradhan Akhilesh , Fazal Muhammad Ali , Patel Akash 
Published: August 2022

AbstractBackground Extracapsular hip fractures comprise approximately half of all hip fractures and the incidence of hip fractures is exponentially increasing. Extramedullary fixation using a dynamic hip screw (DHS) has been the gold standard method of operative treatment for extracapsular fractures, however, in recent years, intramedullary nails (IMN) have become a popular alternative. Intramedullary versus extramedullary fixation is continuously discussed and debated in literature therefore, the purpose of this systematic review and meta-analysis is to directly compare the peri-operative and post-operative outcomes for DHS versus IMN to provide an up-to-date analysis as to which method of fixation is superior. Methods The MEDLINE/Pubmed, Embase and Web of Science Database were searched for eligible studies, from 2008 to August 2021, that compared peri- and post-operational outcomes for patients undergoing IMN or DHS operations for fixation of unstable extracapsular hip fractures (PROSPERO registration ID:CRD42021228335). Primary outcomes included mortality rate and re-operation rate. Secondary outcomes included operation time, blood loss, transfusion requirement, complication, and failure of fixation rate. The risk of bias and quality of evidence were assessed using the Cochrane RoB 2.0 tool and GRADE analysis tool, respectively. Results Of the 6776 records identified, 22 studies involving 3151 patients, were included in the final review. Our meta-analysis showed no significant different between mortality rates (10 studies, OR 0.98; 95% CI 0.80 to 1.22, p = 0.88), and similarly, no significant difference for re-operation rates (10 studies, OR 1.03; 95% CI 0.64 to 1.64, p = 0.91). There was also no significant difference found between complication or failure of fixation rates (17 studies, OR 1.29; 95% CI 0.79 to 2.12, p = 0.31) and (14 studies, OR 1.32; 95% CI 0.74 to 2.38, p = 0.35). Mean blood loss was not included in the meta-analysis but was demonstrated to be significantly greater in those undergoing DHS in 12 out of 13 studies. Conclusion Overall, based on the outcomes assessed, this review has demonstrated no significant difference in the peri- or post-operative outcomes for DHS vs IMN. Future studies should investigate DHS vs IMN for different types of unstable fractures as well as investigating different types and generations of fixation devices.
Item analysis of midterm pediatrics multiple choice exams of college of Medicine KKU: a step in evaluation and improvement of assessment
Authors: Mustafa Alam Eldin Musa 
Published: August 2022

Abstract
Objectives: To evaluate the detailed indices of the analysis of the items of the written multiple choice questions (MCQs) exams in the Department of Child Health, Faculty of Medicine over the last four academic years (1439-1443 AH) and to construct the outlines of a plan for improving the upcoming written MCQs exams. Methods: This was retrospective cross sectional study on the item analysis of the exams in the MBBS course of pediatrics-2 for both boys and girls group in the Child Health Department, Faculty of Medicine, King Khalid University, Saudi Arabia for the midterm MCQs exams in the years of 1439,1440,1441,1442 and the first semester of girl group in 1443. The total number of items studied in these 16 exams were 643 items. The data was obtained constitute the difficulty, discrimination, point biserial reliability and distractor analysis of each of the exams items. The data was tabulated and the statistical significance determined for some variables in the analysis. Results: Students’ scores: were as follows: A scored by 7.3% of students, B by 21.8%, C by 33%, D by21.4% and F by 16.5%. Difficulty index: considering a difficulty index of 80% as easy item, of 30% or less as difficult item and that between 30% and 80% of moderate difficult; we obtain 3 categories of items: difficult percentage is 6.6% of the total items, moderate difficulty represent 53.4% and easy items 40% of the total items. There was significant statistical correlation (p ≤ 0.05) when these difficulty levels compared through over the exam years. Conclusions: Departmental exam committee needs to work comprehensively to improve the difficulty of the exams towards moderate intermediate class also the quality of the questions need extensive work on refining the distractors and revision of the correctness and the suitability of a considerable number of items.
The Utility of Augmented Teledermatology to Improve Dermatologist Diagnosis of Cellulitis: A Cross-Sectional Study
Authors: Creadore Andrew , Manjaly Priya , Tkachenko Elizabeth , Li David G. , Kaffenberger Benjamin , Shinkai Kanade , Rosenbach Misha , Joyce Cara , Mostaghimi Arash 
Published: August 2022

Abstract
Dermatology consultation for cases of presumed cellulitis improves diagnostic accuracy and management. However, access to in-person consultation remains limited, a gap that could be filled with teledermatology. Augmented teledermatology may improve outcomes. In this cross-sectional study, 20 dermatologists (60% of whom reported conducting inpatient consults > 1 month per year) reviewed 10 real-life cases representing either cellulitis or pseudocellulitis as diagnosed by in-person dermatology consultation. For each case, respondents recorded their diagnosis, confidence, and management decisions after viewing the history and standard teledermatology photos, the responses to a physician-reported cellulitis questionnaire, and finally thermal images. Overall mean diagnostic accuracy increased from 84% ± 4% with the history and physical to 89% ± 3% when adding a cellulitis questionnaire and thermal images (p = 0.23). Accuracy for cellulitis cases specifically significantly increased from 76% ± 6–88% ± 4% when adding a cellulitis questionnaire and thermal images (p = 0.049). Accuracy for pseudocellulitis was consistently ≥ 94%. Augmented teledermatology with a standardized questionnaire and thermal images improved diagnostic accuracy for cases of cellulitis and may increase physician confidence. Dermatologists were able to accurately diagnose regardless of experience with inpatient consults, increasing the pool of potential dermatologists who could diagnose cellulitis remotely.
Polygenic risk of type 2 diabetes is associated with Vascular Dementia but not with Alzheimer's Disease: a Prospective Cohort Study
Authors: Dybjer Elin , Kumar Atul , Nägga Katarina , Engström Gunnar , Mattsson-Carlgren Niklas , Nilsson Peter M. , Melander Olle , Hansson Oskar 
Published: August 2022

Abstract
Background and Objectives
Type 2 diabetes and dementia are associated, but their causal relationship is unclear. Genetic studies, i.e. using polygenic risk scores (PRS) or Mendelian Randomization (MR), and validated dementia endpoints with high diagnostic precision (instead of register-based diagnoses) may clarify causal links between these two entities.
Methods
We tested associations between PRS for type 2 diabetes, fasting glucose, fasting insulin and HbA1c on the one hand and dementia on the other in 29139 adults (mean age 55) followed for 20–23 years. Dementia diagnoses were validated and included all-cause dementia, Alzheimer’s Disease (AD) and Vascular Dementia (VaD) as well as mixed dementia. We also tested causal associations between type 2 diabetes and dementia forms through 2-sample-MR analyses.
Results
Seven different PRSs (including SNPs with different significance thresholds) for type 2 diabetes were tested. A PRS including N = 4891 SNPs with p-value < 5e-04 showed strongest association with the different outcomes, including all-cause dementia (Hazard Ratio [HR] 1.11; Bonferroni corrected p = 3.6e-03), mixed dementia (HR 1.18; Bonferroni corrected p = 3.3e-04) and VaD cases (HR 1.28; Bonferroni corrected p = 9.6e-05). Associations were stronger for non-carriers of the AD risk gene APOE ε4. 2-sample-MR analyses could, however, not confirm a causal link between genetic risk markers of type 2 diabetes and dementia outcomes.
Conclusions
Polygenic risk of type 2 diabetes is associated with dementia risk, in particular VaD. Associations were strongest for non-carriers of APOE ε4. However, 2-sample-MR findings did not support a causal relationship between type 2 diabetes and VaD.
Some global topological properties of a free boundary problem appearing in a two dimensional controlled ruin problem
Authors: Grandits Peter 
Published: August 2022

Abstract
In this paper a two-dimensional Brownian motion (modeling the endowment of two companies), absorbed at the boundary of the positive quadrant, with controlled drift, is considered. The volatilities of the Brownian motions are {\em different}. We control the drifts of these processes and allow that both drifts add up to the maximal value of one. Our target is to choose the strategy in a way, s.t. the probability that both companies survive is maximized. It turns out that the state space of the problem is divided into two sets. In one set the first company gets the full drift, and in the other set the second one. We describe some topological properties of these sets and their separating curve.
An improved protocol for efficient regeneration, transformation and gene-editing using CRISPR/Cas9 in pigeonpea (Cajanus cajan(L.) Millsp.)
Authors: Verma Rachana , Bharti Jyotsna , kaul Tanushri , Sony Sonia Khan , Fathy Khaled , Thangaraj Arul Prakash , Nehra Mamta , Easwaran Murugesh , Kaul Rashmi 
Published: August 2022

Abstract
Background: Pigeonpea (Cajanus cajan (L.) Millsp.; 2n = 2× = 22) is a drought-tolerant perennial grain legume commonly cultivated in India's rain-fed and dry land zones, and it is a remarkable natural source of minerals and protein worldwide. Despite decades of research, the constraints linked to tissue culture continues to hinder the genetic improvement of the crop for instances; the explants inability to produce an embryogenic calli, direct shoot formation, and limited regeneration potential, as well as a lack of an effective transformation system. Thereby, efficient calli production, in-vitro recovery, and a systematic technique to co-delivery of Cas9 and sgRNA into the plant remain pre-requisites for efficient genome alteration. Results: To establish a CRISPR/Cas9 based genome editing platform in pigeonpea, a CRISPR/Cas9 mediated binary vector for coherent gene modification (for example, knockout, Homology Donor Repair) was designed. Calli production was achieved at a rate of over 70% by combining with a standardized robust genetic transformation method of pigeonpea using embryonic axis and cotyledonary node explants. The addition of tailored growth regulators and silver nitrate to shoot induction media boosted in-vitro regeneration by up to 89 %. Finally, as a hallmark, for biolistic-mediated transformation, an improved regeneration strategy was used, resulting in the successful incorporation of the transforming binary vector into the embryogenic calli with a transformation efficiency of 46%. PCR and Southern blot analyses were used to validate the results in the end.Conclusion: The standardized shoot regeneration method and successful transformation method of Cas9 in this research, made pioneer steps for facilitating the procedures for the improvement of this crop, which is as yet considered as recalcitrant. Furthering the protocol uncovers significant contributions in both effectiveness and convenience over existing pigeonpea transformation techniques, thereby enabling genetic research on other legumes/dicot crops achievable, as well.
Process optimization of the anaerobic co-digestion of Alternanthera philoxeroides and corn straw enhanced by biochar and Fe3O4
Authors: Fan Qiongbo , Shao Zhijiang , Guo Xiaohui , Zhou Qinqin , Qiu Ling 
Published: August 2022

Abstract

Alternanthera philoxeroides (AP), a highly invasive plant species, poses a serious threat to agriculture, ecological environment. In this study, the methane production properties of the anaerobic co-digestion (coAD) of AP and corn straw (CS) were studied. The interaction effects of total solids content (TS%), biochar, and Fe3O4 on methane yield were evaluated by regression model and response surface method (RSM). The regression model was extremely significant (p < 0.0001). The results show that the order of the effect of three factors on the methane yield of coAD was: TS% > Fe3O4 > biochar. With the aid of RSM, the optimum values of TS%, Fe3O4 and biochar were: 9.67 %, 8.41 g/kg and 5.51 %, respectively. The effects of three factors on the performance of coAD for methane yield were as follows: the content lower than the predicted optimal value was positively correlated with the methane yield, and the content higher than the predicted optimal value was negatively correlated with the methane yield. To improve the methane quality of AP, coAD could be carried out at a lower TS%, biochar and Fe3O4 were added to enhance the methane yield performance. This paper optimized the operational parameters of the coAD of AP and CS, which also provides new strategies for the treatment and utilization of AP.
Soil chemical and physical attributes in recovering areas in the Southern Amazon
Authors: Maria¹² Luciano Souza , Caione³ Gustavo 
Published: August 2022

Abstract

The objective was to evaluate the contribution of soil’s physical and chemical attributes in degraded areas in recovery process with different ages and compared to adjacent degraded areas cultivated with grassland, in the Southern Amazon. For this, was used areas located in the south of the Brazilian Amazon and four different areas were chosen, with 6 years of recovery (APP-6), 3 years (APP-3), degraded (APPD) area and degraded areas cultivated with grassland (DP). With emphasis on the physical variable bulk density soil showed averages of 1.37 ± 0.052 g cm− 3(APP-6), 1.49 ± 0.066 g cm− 3(APP-3), 1.55 ± 0.055 g cm− 3(APPD) and 1.67 ± 0.077 g cm− 3(DP), respectively, and there was a decrease in soil density with longer area recovery time. As for the soil chemical variable of P observed averages of 18.46 ± 2.74 mg kg− 1(APP-6), 2.86 ± 1.73 mg kg− 1(APP-3), 1.46 ± 0.69 mg kg− 1(APPD) and 1.1 ± 0.20 mg kg− 1(DP), there was a high increase in P in areas with longer recovery time. Study of comparison of means, was possible to elucidate the relationships between the soli’s chemical and physical attributes in the four areas studied. The results showed a greater supply of organic carbon, phosphorus, exchangeable cations (K+, Ca+ 2and Mg+ 2). Besides the improvement in the physical attributes of total porosity and the bulk density of the soil in the area of ​​APP-6 years of recovery, in comparison with the other areas
Construction of gene network database, and identification of key genes for diagnosis, prognosis, and treatment in sepsis
Authors: Li Qingsheng , Qu Lili , Miao Yurui , Li Qian , Zhang Jing , Zhao Yongxue , Cheng Rui 
Published: August 2022

AbstractSepsis and sepsis-related diseases cause a high rate of mortality worldwide. The molecular and cellular mechanisms of sepsis are still unclear. Six sepsis-related blood transcriptome datasets were collected and analyzed by weighted gene co-expression network analysis (WGCNA). Functional annotation was performed in gProfiler tool. DSigDB was used for drug signatures enrichment analysis. The proportion of immune cells was estimated by the CIBERSORT tool. The relationships between modules, immune cells, and survival were identified by correlation analysis and survival analysis. A total of 37 stable co-expressed gene modules were identified. These modules were associated with the critical biology process in sepsis. Four modules can independently separate patients with long and short survival. Three modules can recurrently separate sepsis and normal patients with high accuracy. Some modules can separate bacterial pneumonia, influenza pneumonia, mixed bacterial and influenza A pneumonia, and non-infective systemic inflammatory response syndrome (SIRS). Drug signature analysis identified drugs associated with sepsis, such as testosterone, phytoestrogens, ibuprofen, urea, dichlorvos, potassium persulfate, and vitamin B12. Finally a gene co-expression network database was constructed (https://liqs.shinyapps.io/sepsis/). In conclusion, the recurrent modules in sepsis may facilitate disease diagnosis, prognosis, and treatment.