bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of High Carbohydrate Intake on Physical Frailty in Older Korean Adults: a Cohort-based Cross-sectional Study
Authors: Yang Narae , Lee Yunhwan , Kim Mi Kyung , Kim Kirang 
Published: January 2022

Abstract
Background: The relationship between macronutrients and frailty is unclear. Previous studies have confirmed the relationships between energy and protein intake and physical frailty, while few studies have examined the role of carbohydrate or fat intake in the prevalence of frailty. The aim of this study is to investigate the relationship of energy and macronutrients with physical frailty in the Korean elderly population who had a high proportion of energy intake from carbohydrates.Methods: This study included 954 adults aged 70 to 84 years who have completed the assessment of frailty and 24-h recall upon enrolment in the Korean Frailty and Aging Cohort Study and have no extreme intake under 400 kcal (n = 2). The relationship between energy or macronutrients and frailty was evaluated using multivariate logistic regression models and multivariate nutrient density models.Results: In the subjects with low energy intake (odds ratio [OR] = 2.94, 95% confidence interval [CI] = 1.34–6.45) and total subjects (OR = 2.01, 95% CI = 1.03–3.93), consuming carbohydrates above the acceptable macronutrient distribution range (65% of energy) was related to a higher risk of frailty. Substituting the energy from fat with carbohydrates was related to a higher risk of frailty (1%, OR = 1.05, 95% CI = 1.00–1.09; 5%, OR = 1.26, 95% CI = 1.02–1.56; 10%, OR = 1.59, 95% CI = 1.03–2.43).Conclusions: This study showed that the proportion of energy intake from carbohydrates and fats may be an important nutritional intervention factor for reducing the risk of frailty.
An Interval Type-2 Adaptive Neuro-Fuzzy Inference System Based, Artificial Pacemaker Design and Stability Analysis
Authors: Dabiri Asghar , Dabanloo Nader Jafarnia , Nooshiravan Fereidoun , Maghooli Keivan 
Published: January 2022

Abstract
This paper presents design and simulation of an Interval type-2 fuzzy system (IT2FS) based, Adaptive neuro-fuzzy inference system(ANFIS) pacemaker controller in MATLAB. After designing the type-1 fuzzy logic model, the stability of the designed system has been verified in the time-domain (unit step response). In previous works, type-1 (IT1FS) model step response was analyzed and compared with the other PID and Fuzzy models that only least-square-estimation and the backpropagation algorithms are used for tuning membership functions and generation of type-1 fis (fuzzy inference system) file, but at current work Fuzzy C Means (FCM) method that shows better results has been used. The pacemaker controller determines the pacing rate and adjusts the heart rate of the patient with respect to the reference input signal. The rise-time, overshoot and settling-time have been improved significantly.
Effectiveness of Physiotherapy to Promote Motor Recovery in Individuals with Stroke: A Systematic Review Protocol
Authors: Zhang Shehong , Xie Hongyu , Wang Chuanjie , Wu Fengfeng , Wang Xin 
Published: January 2022

Abstract
Introduction: Motor function is essential in our daily lives, one of the most common impairments caused by stroke is loss of functional movement. Over 70% of stroke survivors have motor or other neurological functional disabilities. However, rehabilitation of motor function suffered from a stroke can be rather difficult due to the complexity of organs and systems related to motor function, as well as the neural system that supported motor function. In particularly, previous evidence for the effectiveness of physiotherapy, a commonly prescribed intervention method for people with stroke, that recover motor function in people following a stroke is varied and limited in the chronic rehabilitation phase and therefore has never been reviewed systematically. With the progress of study in neurology and the development of novel tools for rehabilitation, results from more and more clinical trials are now available, thus here justifying conducting a systematic review. Methods and analysis: This systematic review protocol is developed in accordance with the methodology recommended by the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols, as well as the Cochrane handbook for systematic reviews of interventions. Relevant studies will be identified by searching the databases. We will perform searches for relevant studies in databases, including PubMed, Embase, CINAHL, and Web of Science, Physiotherapy Evidence Database and Cochrane Library databases. The reference lists of included articles and reviews will be searched manually. The date range parameters used in searching all databases will be restricted between January 2001 and January 2021. Randomized controlled trials (RCTs) published will be included. The language used in the articles included was restricted to English. The GRADE (Grading of Recommendations, Assessment, Development and Evaluation system from the Cochrane Handbook for Systematic Reviews of Interventions) approach will be used to systematically appraise the quality of methodology. We will assess the risk of bias of the RCTs included using the Cochrane Collaboration’s tool and provide a qualitative synthesis. After that, we will consider conducting a meta-analysis if the final data across outcomes shows sufficient homogeneity. Ethics and dissemination: No ethical approval is needed as the proposed study does not involve the collection of primary data, and the results of this review will be disseminated via peer-reviewed publications and conference presentations. Trial registration number: CRD42021267069.
Range Shifts of Overwintering Birds Depend on Habitat Type, Snow Conditions and Habitat Specialization
Authors: Bosco Laura , Xu Yanjie , Deshpande Purabi , Lehikoinen Aleksi 
Published: January 2022

Abstract
Climatic warming is forcing numerous species to shift their ranges poleward, which has been demonstrated for many taxa across the globe. Yet, the influence of habitat types on within- and among-species variations of distribution shifts has rarely been studied, especially so for the non-breeding season. Here, we investigated habitat specific shift distances of northern range margins and directions of the center of gravity based on a long-term dataset of overwintering birds in Finland. Specifically, we explored influences of habitat type, snow cover depths, species’ climatic niche and habitat specialization on range shifts from 1980’s to 2010’s in 81 bird species. Birds overwintering in farmlands shifted significantly more often northwards than birds of the same species in rural and forest habitats, while the northern range margin shift distances did not significantly differ among the habitat types. Snow cover was negatively associated with the eastward shift direction across all habitats, while we found habitat specific relations to snow cover with northward shift directions and northern range margins shift distances. Species with stronger habitat specializations shifted more strongly towards north as compared to generalist species, whereas the climatic niche of bird species only marginally correlated with range shifts, so that cold-dwelling species shifted longer distances and more clearly eastwards. Our study reveals habitat specific patterns linked to snow conditions for overwintering boreal birds and highlights importance of habitat availability and preference in climate driven range shifts.
Predictors of In-Hospital Mortality in Stroke Patients
Authors: Ranasinghe Vindya Shalini , Pathirage Manoji , Gawarammana Indika Bandara 
Published: January 2022

Abstract
Background: In-hospital mortality is a good indicator to assess the efficacy of stroke care. Identifying the predictors of in-hospital mortality is important to advance the stroke outcome and plan the future strategies of stroke management. Methods: This was a prospective observational study conducted at a tertiary referral center in Sri Lanka to identify the possible predictors of in-hospital mortality. The study included 246 confirmed stroke patients. The diagnosis of stroke was established on the clinical history, examination and neuroimaging. The differentiation of stroke in to haemorrhagic type and ischaemic type was based on the results of computed tomography. In all patients, demographic data, comorbidities, clinical signs (pulse rate, respiratory rate, systolic blood pressure, diastolic blood pressure, on admission Glasgow Coma Scale (GCS) score) and imaging findings were recorded. Serum electrolyte test was performed in all stroke patients and hyponatremia was defined as serum Na+ less than 131mmol/l. All patients were followed up throughout their hospital course and the in-hospital mortality was recorded. In hospital mortality was defined as the deaths which occurred due to stroke after 24 hours of hospital admission. Results: The incidence of in-hospital mortality was 11.7% (95% confidence interval 8-16.4). The mean day of in-hospital deaths to occur was 5.9 days (SD±3.8 Min 2 Max 20). According to multivariate logistic regression analysis on admission GCS score (Odds Ratio (OR)-0.71) and haemorrhagic stroke type (OR-5.12) predict the in-hospital mortality. The area under the curve of receiver operating curve drawn for the on admission GCS score was 0.78 with a sensitivity of 96.31% and specificity of 41.38% for a patient presented with the GCS score of <10. Conclusion: On admission GCS and haemorrhagic stroke are independent predictors of in-hospital mortality. Patients with on admission GCS <10 have a moderate predictive ability in predicting the in-hospital mortality. Thus, a special attention should be given to the patients with low GCS score and haemorrhagic strokes for reducing rates of in-hospital mortality.
Self-Management Behaviours and Change in Frailty Status among Community-Dwelling Older Adults: A National Longitudinal Population-based Cohort Study
Authors: To Thi Lien , Kuo Ching-Pyng , Yeh Chih-Jung , Liao Wen-Chun , Lee Meng-Chih 
Published: January 2022

Abstract
Background: Frailty in older adults is a common geriatric syndrome that can be reversed, thus coping strategies for the aging population are essential. Self-management behaviours may represent cost-effective strategies to reverse physical frailty in community-dwelling older adults. This study aimed to describe the changes in frailty status among community-dwelling older adults in Taiwan and investigate the association of self-management behaviours with changes in frailty status over a four-year follow-up period (2007 to 2011).Methods: This data was retrieved from the Taiwan Longitudinal Study of Aging (TLSA), which is a prospective cohort study of 1,283 community-dwelling older adults aged 65 years and older without cognitive impairment. Frailty was assessed based on Fried's frailty phenotype, in which ≥ three criteria indicate frail. Self-management behaviours (maintaining body weight, quitting smoking, drinking less, exercising, diet control, and maintaining a regular lifestyle) were assessed using a questionnaire. Multivariate logistic regression analyses were used to investigate the associations between self-management behaviours and changes in frailty status.Results: The prevalence of frailty was 8.7% at baseline and 8.1% after four years of follow-up, with 196 (15.3%) deaths. Overall, 74.6% of participants remained in the same state (non-frail or frail), 23.5% worsened (non-frail to frail, including missing data, and frail to death), and only 1.95% improved (frail to non-frail). Being aged ≥ 75-years-old, chronic diseases, and an absence of self-management behaviours were associated with higher risks of frailty at baseline and after follow-up. Exercise was significantly associated with a reversal of frailty in community-dwelling older adults (RR, 3.11; 95% CI, 1.95, 4.95) after adjusting for personal and disease covariates, regardless of whether death was coded as frail or not.Conclusions: Self-management behaviours beneficially reverse frailty status; maintaining regular exercise was especially associated with a reversal of frailty in community-dwelling older adults, even among individuals over 75-years-old and with chronic diseases. Older adults should be encouraged to perform adequate physical exercise to prevent the progression of frailty and ameliorate frailty status.
Inhibition of Voltage-Gated Hv1 Alleviates LPS-Induced Neuroinflammation via Regulation of Microglial Metabolic Reprogramming
Authors: Sun Lingbin , Wang Xihua , Guan Shuyuan , LUO TAO 
Published: January 2022

Abstract
Background
Neuroinflammation plays an important role in the onset and advancement of cognitive loss and neurodegenerative disorders. The voltage-gated H channel (Hv1) has been reported to be involved in microglial activation and act as key drivers of neuroinflammation. This study aims at evaluating the mechanism of Hv1 involvement in neuroinflammation and the therapeutic potential of Hv1 inhibitor, 2-guanidinobenzimidazole (2-GBI), in a model of lipopolysaccharide (LPS)-induced neuroinflammation.
Methods
We investigated the influence of Hv1 inhibitor (2-GBI) on the generation of reactive oxidative species (ROS), metabolic reprogramming, and inflammatory mediators in vitro and examined the therapeutic potential of 2-GBI on microglial activation and hippocampal neuroinflammation in vivo. Novel object recognition and Y-maze were employed to assess cognitive function.
Results
2-GBI reduced the LPS-induced proinflammatory response and aerobic glycolysis in microglia. HIF1α overexpression mediated aerobic glycolysis reprogramming alleviated by 2-GBI. We reported that Hv1 inhibitor exerted a protective effect on LPS-induced neuroinflammation through the ROS/HIF1α and PI3K/AKT/HIF1α pathways -mediated aerobic glycolysis. The cell death of PC12 induced by microglia-mediated neuroinflammation was reversed in a transwell co-culture system by 2-GBI. Furthermore, in vivo results suggested that 2-GBI mitigated the neuroinflammatory processes and recognition injury through regulation of microglial metabolic reprogramming.
Conclusion
2-GBI protects LPS-induced neuroinflammation, neuronal cell death, and subsequently reverses the hippocampus-dependent cognitive deficits through regulation of microglial metabolic reprogramming. Taken together, these results demonstrate a key role for Hv1 in driving a pro-inflammatory microglia phenotype in neuroinflammation.
Hypoxia-related lncRNA Signature to Predict the Survival of Patients with Clear Cell Renal Cell Carcinoma
Authors: Liu Fu , Li Xinyuan , Zhou Xiang , Tong Hang , Gou Xin 
Published: January 2022

Abstract
Background: Renal cell carcinoma is the most common aggressive tumor of the genitourinary system. The main pathological subtype is clear cell renal cell carcinoma (ccRCC), and its treatment options are very limited. Therefore, identifying specific markers of renal clear cell carcinoma is of great significance for diagnosis and prognosis.Methods: From the TCGA database, we obtained information on 611 patients with renal clear cell carcinoma to analyze the relationship between hypoxia-related lncRNAs and overall survival. According to the coexpression of hypoxia genes and lncRNAs, genes related to hypoxia were identified. Difference analysis and Cox regression analysis were applied to assess survival-related risk factors. According to the median risk score of hypoxia-related genes, patients were divided into high-risk and low-risk groups. According to these gene characteristics and clinical parameters, a nomogram map was built, and GSEA was used for gene function annotation. RT-qRCR, Western Blot and Flow Cytometry were used to determine the role of SNHG19 in RCC cells.Results: By analyzing the coexpression of hypoxia genes and lncRNAs, 310 hypoxia-related genes were obtained. Six sHRlncRs were significantly correlated with the clinical outcomes of patients with ccRCC. Four sHRlncRs (AC011445.2, PTOV1-AS2, AP004609.3, and SNHG19) with the highest prognostic values were included in the group to construct the HRRS model. The high-risk group had a shorter OS than the low-risk group. HR-lncRNAs were considered to be an independent prognostic factor and associated with OS. The high- and low-risk groups showed different pathways in GSEA. Experiments showed that SNHG19 plays essential roles in autophagy and apoptosis of RCC cells.Conclusion: Our research shows that we established and verified a hypoxia-related lncRNA model that accurately correlates with ccRCC patients. This study also provides novel insights into hypoxia-based mechanisms and provides new biomarkers for the poor prognosis of ccRCC patients.
The vacuolar iron transporter mediates iron detoxification in Toxoplasma gondii
Authors: Aghabi Dana , Sloan Megan , Dou Zhicheng , Antipova Olga , Guerra Alfredo , Harding Clare 
Published: January 2022

Abstract
Iron is essential to living cells, acting as a cofactor in a number of important enzymes in metabolism; however in the absence of correct storage iron forms dangerous oxygen radicals. In both yeast and plants, iron is stored in a membrane-bound vacuole through the action of a vacuolar iron transporter (VIT). This transporter is conserved in the apicomplexan family of obligate intracellular parasites, including in Toxoplasma gondii, a pathogen of medical and veterinary importance. Here, we assess the role of VIT, and iron storage, in T. gondii. We show that iron is restricted to a compartment in the parasite that does not overlap with zinc. By deleting VIT we find a slight growth defect in vitro, however the absence of VIT leads to hypersensitivity to iron, confirming its essential role in iron detoxification in the parasite. This hypersensitivity can be rescued by scavenging of oxygen radicals. In the absence of VIT, parasites store less iron and are at a growth disadvantage when moving into an iron-depleted environment. We show parasite VIT expression is regulated by iron levels at both the transcript and protein level, and by altering the distribution of VIT within the cell. In the absence of VIT, we find that T. gondii responds by altering expression of genes with a role in iron metabolism and by increasing the activity of the antioxidant protein catalase. We also show that iron detoxification has an important role both in parasite survival within macrophages and in virulence in a mouse model. Together, by demonstrating a critical role for VIT during iron detoxification in T. gondii, we reveal the importance of iron storage in the parasite and provide the first insight into the machinery involved.
Surface Water Quality Assessment, Prediction &amp; Modelling of River Daya in Odisha
Authors: Jena Pramod , Rahaman *Sayed Modinur , DasMohapatra Pradeep Kumar , Barik Durga Prasad , Patra Dikshya Surabhi 
Published: January 2022

Abstract
A decision tree -based approach is projected to predict surface water quality and is a good tool to assess quality and guarantee property safe use of water for drinking. The most objective of this study is to assess the surface water quality of the Daya watercourse to work out the quality of drinking functions. Samples were collected from designated locations throughout totally different seasons (winter, summer, rainy) over a amount of five years (2016, 2017, 2018, 2019, and 2020). Total dissolved solids, pH, alkalinity, chloride, nitrate, total hardness, calcium, magnesium, iron, fluoride, were all tested as well as total coliform, fecal coliform, and E. coli. The main goal is to use decision tree regression to construct a model to assess and predict water quality changes in the Daya geographic region of Odisha, India, and compare it to statistical methods.
COVID-19’s Impact on Visitation Behavior to US National Parks From Communities of Color - Evidence From Mobile Phone Data
Authors: Alba Charles , Pan Bing , Yin Junjun , Rice William L. , Mitra Prasenjit , Lin Michael , Liang Yun 
Published: January 2022

Abstract
The widespread COVID-19 pandemic fundamentally changed many people’s ways of life. With the necessity of social distancing and lock downs across the United States, evidence shows more people engage in outdoor activities. With the utilization of location-based service (LBS) data, we seek to explore how visitation patterns to national parks changed among communities of color during the COVID-19 pandemic. Our results show that visitation rates to national parks located closer than 347km to individuals have increased amidst the pandemic, but the converse was demonstrated amongst parks located further than 347km from individuals. More importantly, COVID-19 has adversely impacted visitation figures amongst non-white and Native American communities, with visitation volumes declining if these communities are situated further from national parks. Our results show disproportionately low-representations amongst national park visitors from these communities of color. African American communities display a particularly concerning trend whereby their visitation to national parks is substantially lower amongst communities closer to national parks.
SPAG5 Expression Correlated With Prognostic Implication and Immune Infiltration in Lung Adenocarcinoma
Authors: Xie Jianjiang , Xu Xie , Zhou Huaping 
Published: January 2022

Abstract
Background Sperm-associate antigen 5 (SPAG5) is a critical oncogene in several cancers. But the role of SPAG5A in lung adenocarcinoma (LUAD) remains unclear. Thus, the aims of our study are to explore the function and underlying mechanism of SPAG5 in LUAD. Methods Expression of SPAG5 was determined using the Oncomine, TIMER, and GEPIA databases. Correlation of SPAG5 and survival was detected by GEPIA database, PrognoScan, Kaplan-Meier Plotter databases. And the association between SPAG5 and tumor malignant phenotypes were analyzed by the CancerSEA. Besides, the correlation between SPAG5 expression and tumor immune infiltration as well as immune checkpoints were analyzed by TIMER. the co-expression genes of SPAG5 were identified using STRING, and the mutation and biological function of SPAG5 and its co-expression genes were determined by cBioPortal and Metascape, respectively. Finally, the SPAG5 expression in LUAD samples was determined by tissues microarrays (TMA) and immunohistochemistry (IHC) analyses.Results We found that upregulated SPAG5 associated with poor survival of LUAD patients. Besides, SPAG5 expression associated to B cell, CD4+ cell, CD8+ cell, macrophage, DC cell as well as CD274, CTLA4, GZMB, LAG3, PDCD1, TIGIT in LUAD. SPAG5 expression also associated with cell proliferation, cell cycle, DNA damage and repair, epithelial-mesenchymal transition (EMT), invasion, and stemness, inflammation in LUAD. Conclusion Our finding indicated that SPAG5 acted as a crucial oncogene in LUAD, and correlated with unfavorable survival as well as tumor infiltration inflation.