bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drp1‐Dependent Mitochondrial Fission Contributes to Lactic Acid‐Induced Chicken Cardiomyocyte Damage
Authors: Hu Dongfang , Cui Yunli , Hou Xueke , Wang Xueying , Shen Zihui , Pang Huiqing , Ge Yaming , Ning Hongmei 
Published: January 2025

ABSTRACTEnhanced glycolysis and elevated lactic acid (LA) production are observed during sudden death syndrome (SDS) in broilers. However, the mechanism underlying LA‐induced cardiomyocyte damage and heart failure in fast‐growing broilers remains unclear. In this study, chicken embryo cardiomyocytes (CECs) were cultured and treated with LA to investigate LA‐induced CEC injury and its mechanism, aiming to develop strategies to prevent LA‐induced SDS in broilers. Results showed that LA inhibited CEC proliferation and contraction whereas inducing apoptosis. Furthermore, LA disrupted mitochondrial ultrastructure, reduced mitochondrial membrane potential, activated mitophagy, and disturbed mitochondrial dynamics. Treatment with Mdivi‐1, a selective Drp1 inhibitor, improved CEC viability, restored mitochondrial network integrity, reduced reactive oxygen species production, and inhibited LA‐induced apoptosis. These findings suggest that LA‐induced cardiomyocyte injury during SDS in broilers is associated with mitochondrial damage and increased mitochondrial fission. The inhibition of mitochondrial hyperfission by Mdivi‐1 effectively preserves CEC morphology, structure, and function, playing a critical role in preventing LA‐induced damage. This study provides a foundation for strategies to prevent and control SDS in broilers.
Characteristics of eligible patients with knee osteoarthritis accepting versus declining participation in a randomised trial investigating the effect of weight loss versus knee arthroplasty to explore generalisability: A cross‐sectional study
Authors: Aljuboori Saber Muthanna , Christensen Robin , Henriksen Marius , Bliddal Henning , Troelsen Anders , Boesen Mikael , Poulsen Asbjørn Seenithamby , Nielsen Camilla Toft , Bunyoz Kristine Ifigenia , Overgaard Søren 
Published: January 2025

AbstractBackgroundThe INtensive diet versus Knee Arthroplasty (INKA) trial is a randomised trial assessing weight loss as an alternative to knee arthroplasty (KA) in obese patients with severe knee osteoarthritis (OA) awaiting KA (NCT05172843). The external validity of the INKA trial may be hampered if the patients who participate differ from those who decline participation.ObjectiveTo compare baseline characteristics between patients who enrol in the INKA trial and those who decline participation (i.e., non‐INKA [nINKA] group).MethodsWe applied a cross‐sectional study design, collecting and comparing baseline characteristics among all patients eligible for enrolment in the INKA trial from two clinics in Copenhagen. Imbalance between accepting (INKA) and declining (nINKA) groups was assessed using standardised differences (StdDs). We were prespecified that StdD values < 0.20 would indicate a clinically insignificant imbalance between groups, whereas values > 0.80 indicate incomparability.ResultsOf the 913 patients scheduled for KA, 888 were screened for INKA trial eligibility. Of the 217 eligible patients, 92 (42%) were enroled in the INKA trial, while 37 (17%) participated in the nINKA cross‐sectional sample only. Patients enroled in INKA had on average a less severe Oxford knee score (OKS) of 22.0 (standard deviation = 6.7) compared to declining participants in nINKA with 18.6 (7.2), corresponding to an StdD of 0.50, and an absolute difference of 3.45 (95% confidence interval = 0.64–6.26, p = 0.017). A consistent similar pattern was noted across all secondary patient‐reported outcomes applied in the INKA trial.ConclusionsWe observed discrepancies in patient‐reported outcomes, with those who declined enrolment reporting more severe symptoms. These differences, however, were below the minimally important difference between groups for OKS, which is set to 4.84 points.Level of EvidenceLevel II–III cross‐sectional study in a randomised control trial.
Multiple secondary stabiliser injuries increase rotational instability in anterior cruciate ligament‐deficient knees
Authors: Kato Jiro , Fukushima Hiroaki , Kensaku Abe , Hanaki Syunta , Ota Kyohei , Kawanishi Yusuke , Kobayashi Makoto , Yoshida Masahito , Takenaga Tetsuya , Kawaguchi Yohei , Kuroyanagi Gen , Sakai Hiroaki , Murakami Hideki , Nozaki Masahiro 
Published: January 2025

AbstractPurposeMedial meniscus ramp lesions (MMRLs), lateral meniscus posterior root tears (LMPRTs), and anterolateral complex injuries (ALCIs) are major secondary stabiliser injuries associated with anterior cruciate ligament (ACL) injuries. This study aimed to investigate the effect of the number of secondary stabiliser injuries on knee instability in ACL injuries.MethodsPatients who underwent primary ACL reconstruction between January 2017 and May 2023 were enroled in this study. Exclusion criteria encompassed patients with other ligament injuries, a history of contralateral knee injury, hyperextension, flexion contracture and meniscus injuries other than MMRL or LMPRT. Ultimately, 158 patients (mean age: 25.3 years; 81 males and 77 females) were included in this study, and the presence of MMRL, LMPRT and ALCI was investigated. Patients were categorised into four groups based on the number of ACL and secondary stabiliser injuries: single (isolated ACL injury), dual, triad and tetrad. Subsequently, the groups were compared regarding pivot shift grade, quantitative rotational instability measured using an inertial sensor, and anterior tibial translation (ATT).ResultsSecondary stabiliser injuries identified included ALCI 85 (53.8%), MMRL 58 (36.7%) and LMPRT 23 (14.6%). The distribution of patients in the single, dual, triad and tetrad groups was 45 (28.5%), 68 (43.0%), 37 (23.4%) and 8 (5.1%), respectively. High‐grade pivot shifts were observed in 33.3% (15 out of 45) of the single group, 63.2% (43 out of 68) of the dual group, 67.6% (25 out of 37) of the triad group, and 100% (8 out of 8) of the tetrad group. Quantitative evaluations using the inertial sensor revealed significantly lower acceleration in the isolated ACL injury group compared to the other groups (p < 0.05). No significant difference was observed in ATT measurements (n.s.).ConclusionThe combination of secondary stabiliser injuries led to higher instability. Therefore, it is important to carefully diagnose these injuries and devise appropriate treatment plans, particularly in cases of high knee instability.Level of EvidenceLevel III diagnostic.
Meniscal forces and knee kinematics are affected by tibial slope modifying high tibial osteotomy
Authors: Winkler Philipp W. , Chan Calvin K. , Polamalu Sene K. , Lucidi Gian Andrea , Wagala Nyaluma N. , Hughes Jonathan D. , Debski Richard E. , Musahl Volker 
Published: January 2025

AbstractPurposeTo quantify the effect of increasing the posterior tibial slope (PTS) on knee kinematics and the resultant medial and lateral meniscal forces.MethodsIn this controlled laboratory study, a 6 degrees of freedom (DOF) robotic testing system was used to apply external loading conditions to seven fresh‐frozen human cadaveric knees: (1) 200‐N axial compressive load, (2) 5‐N m internal tibial +10‐N m valgus torque and (3) 5‐N m external tibial + 10‐N m varus torque. Knee kinematics and the resultant medial and lateral meniscal forces were acquired for two PTS states: (1) native PTS and (2) increased PTS. Resultant forces in the medial and lateral meniscus were calculated using the principle of superposition.ResultsIn response to 5‐N m external tibial + 10‐N m varus torque, significantly more internal tibial rotation was observed after increasing PTS at 60° (p = 0.0156) and 90° (p = 0.0156) flexion. Increasing PTS caused significantly more medial tibial translation from 30° to 90° flexion in response to 5‐N m internal tibial + 10‐N m valgus torque. In response to 5‐N m external tibial + 10‐N m varus torque, the resultant force in the medial meniscus at 60° flexion decreased significantly after increasing PTS (32.8%, p = 0.016). Resultant forces in the lateral meniscus decreased significantly after increasing PTS at 30° (34.5%; p = 0.016) and 90° (29.7%; p = 0.031) flexion in response to 5‐N m internal tibial + 10‐N m valgus torque.ConclusionIncreasing PTS in a native knee with intact cruciate ligaments affected 6 DOF knee kinematics and decreased resultant forces in the medial and lateral meniscus by up to 35% in response to combined rotatory loads. Therefore, increasing PTS during high tibial osteotomy in a knee with intact cruciate ligaments does not increase the force carried by the entire meniscus at time zero.Level of EvidenceN/A.
Initial Boundary Value Problem for Partial Differential–Algebraic Equations With Parameter
Authors: Assanova Anar T. 
Published: January 2025

ABSTRACTThe paper addresses an initial boundary value problem for a partial differential–algebraic equation involving a parameter. An integral condition with respect to the time derivative of the unknown function is provided as an additional condition to determine this parameter. The Dzhumabaev parameterization method is employed to solve the problem. The domain is subdivided, and functional parameters are defined as the values of the solution along the internal lines of the subdomains. This reformulates the original problem into an equivalent initial boundary value problem for a system of hyperbolic equations with parameters and associated functional relations. The paper develops algorithms to solve the problem, demonstrating their applicability. Furthermore, conditions for the existence and uniqueness of a solution to the initial boundary value problem, involving the partial differential–algebraic equation with a parameter and discrete memory, are established.
National and Regional Trends in Congenital Cytomegalovirus Infection From 1998 to 2019
Authors: So Raymond J. , Noij Kimberley S. , Wang Jiangxia , Walsh Jonathan , Gourin Christine G. , Jenks Carolyn M. 
Published: January 2025

AbstractObjectiveTo analyze temporal changes and to assess the possible effect of newborn hearing screening (NBHS) programs on changes in congenital cytomegalovirus (cCMV) diagnostic rates in the United States.Study DesignCross‐sectional study.SettingNational Inpatient Sample (NIS) database.MethodsNeonates with cCMV infection were identified using discharge data from the NIS database from 1998 to 2019. Neonates with cCMV infection were identified via International Classification of Diseases codes and categorized as asymptomatic versus symptomatic and with or without hearing loss. Linear regression and interrupted time series analyses were conducted to analyze changes in diagnostic rates over time. Interrupted analyses were based on the timing of NBHS implementation in geographic regions.ResultsPer 1,000,000 live births, the estimated total number of cCMV diagnoses increased from 109 in 1998 to 250 in 2019 (the estimated annual increase per 1,000,000 live births is 6.89 ([95% confidence interval, 5.43‐8.35], P < .001). Diagnosis of cCMV with hearing loss showed a significant annual increase during this time (0.87 [0.51‐1.22], P < .001), and within this group, diagnosis of both asymptomatic (0.18 [0.03‐0.32], P = .02) and symptomatic (0.68 [0.37‐0.99], P < .001) cases increased significantly. Compared to pre‐NBHS, the rate of increase in cCMV diagnosis was significantly higher post‐NBHS implementation in the Northeast (P < .001) and South (P = .008).ConclusionImplementation of state NBHS programs correlated with increasing diagnosis rates of cCMV, though cCMV education and awareness may be contributing. cCMV continues to be underdiagnosed in a large national database.
Evidence‐Based Medicine in Otolaryngology Part 17: A Qualitative Research Primer
Authors: Raol Nikhila , Pattisapu Prasanth , Ikeda Allison K. , Lowers Jane , Joe Stephanie , Shin Jennifer J. 
Published: January 2025

AbstractAs qualitative data analysis becomes more frequently applied within the fields of medicine and surgery, it is beneficial to expand our knowledge of the utility, methodology, and interpretation of these techniques. While qualitative research serves a distinct purpose and differs from quantitative research, both can be approached with diligence, with careful techniques that are similarly maintained and respected. In advance of this installment in the Evidence‐Based Medicine in Otolaryngology series, we presented a comparison between qualitative and quantitative methods. To provide additional understanding of the specifics of qualitative research, this article presents further insights into qualitative research applications, theories, data collection methods, sources of and approaches to bias, and methods which support rigorous results.
Mycobacterium goodii pulmonary disease with organizing pneumonia: A case report and review of literature
Authors: Shionoya Yu , Kasai Hajime , Kono Reiya , Hirama Ryutaro , Ota Masayuki , Naito Akira , Abe Mitsuhiro , Kawasaki Takeshi , Ikeda Jun‐ichiro , Suzuki Takuji 
Published: January 2025

AbstractMycobacterium goodii, a rapidly growing non‐tuberculous mycobacterium, rarely causes pulmonary diseases. A patient was admitted to our hospital with a fever and cough. Chest radiography revealed consolidation in the right middle lung. As he had previously been treated for organizing pneumonia (OP), he was diagnosed with OP recurrence and administered systemic corticosteroids. Although initial improvement was observed, the pulmonary consolidations worsened. Transbronchial lung cryobiopsy revealed an OP pattern. M. goodii was identified in sputum acid‐fast bacilli cultures. The patient was diagnosed with M. goodii pulmonary disease and secondary OP. Although intravenous imipenem‐cilastatin, amikacin, and ciprofloxacin led to initial improvement in pulmonary consolidations, the consolidations re‐worsened. Systemic corticosteroids were initiated, resulting in improvement in the consolidations. The dose of systemic corticosteroids was tapered; oral antimycobacterial therapy was continued. M. goodii can cause pulmonary disease and induce OP; antimycobacterial therapy and systemic corticosteroids can be effective.
Cutting rule for in-in correlators and cosmological collider
Authors: Ema Yohei , Mukaida Kyohei 
Published: January 2025

Abstract
We derive a cutting rule for equal-time in-in correlators including cosmological correlators based on Keldysh r/a basis, which decomposes diagrams into fully retarded functions and cut-propagators consisting of Wightman functions. Our derivation relies only on basic assumptions such as unitarity, locality, and the causal structure of the in-in formalism, and therefore holds for theories with arbitrary particle contents and local interactions at any loop order. As an application, we show that non-local cosmological collider signals arise solely from cut-propagators under the assumption of microcausality. Since the cut-propagators do not contain (anti-)time-ordering theta functions, the conformal time integrals are factorized, simplifying practical calculations.
Changes in gene expression align with behavioural differences in reproductive choices in a social beetle
Authors: Regon P. , Schudoma C. , Moncaz A. , Harari A. , Kliot A. 
Published: January 2025

Abstract
Social insects often share their colony with individuals of different relatedness levels and life stages. When no reproductive depression is in place, a female must choose whether to stay with her natal group and possibly limit her reproductive potential or leave to establish a new colony. The factors governing and following this decision are still largely unknown. The date stone beetle Coccotrypes dactyliperda (Curculiondae) resides in galleries carved inside date seeds. Sibling mating is common in this species. While most females in a natal group stay and raise their offspring with their sisters, some females leave to start a colony of their own, regardless of resources’ availability within the natal group. In this study, we hypothesized that dispersing females should present physiological changes consistent with the anticipated challenges, including finding a host and egg laying, while the physiology of females that stay in the natal seed should demonstrate physiology related to cohabiting in an existing nest. To test our hypothesis, we examined the development of ovaries in ‘stayers’ and ‘dispersers’ females. In addition, we sequenced and analysed the transcriptome of pooled groups of ‘stayers’ and ‘dispersers’ sisters. Our physiological and transcriptomic results indicate that females who left the natal seed exhibit greater ovarian development. Our transcriptomic data revealed further differences between the two groups, including higher levels of expression of cuticle-related genes in the females that chose to stay in the natal nest. We discuss the possible roles of these genes in the continued life inside the natal nest.
More micrometastases, more recurrence? The role of qPCR of PSA mRNA in lymph nodes during prostatectomy
Authors: Troidl Johannes , Fehr Alexander , Jandrig Burkhard , Köllermann Jens , Lux Anke , Baumunk Daniel , Gür Melis , Liehr Uwe-B. , Porsch Markus , Wendler Johann J. , Blaschke Simon , Schostak Martin 
Published: January 2025

Abstract
Background and objectives
Radical prostatectomy is a standard treatment for prostate cancer, yet about 30% of patients experience rising biochemical markers within a decade post-surgery. Pelvic lymph node sampling during prostatectomy assesses potential lymph node metastases, but standard histological assessments, which typically examine only 2–3 tissue sections, often miss occult metastases. This study assesses the effectiveness of qPCR in detecting PSA coding KLK3 mRNA for identifying lymph node metastases post-prostatectomy and explores the correlation between PSA-mRNA and biochemical recurrence.

Methods
A cohort of 157 patients who underwent radical prostatectomy with lymphadenectomy were examine. On average, 24.7 lymph nodes were removed per patient. Among them, 108 patients reached PSA value below 0.1 ng/ml without receiving additional therapy, and 106 were followed up over a duration of 5.4 years. This subgroup is of particular interest because it allows for the investigation of the correlation between the occurrence of PSA-mRNA in lymph nodes and later biochemical recurrence.
Key findings and limitations
qPCR of PSA-mRNA identified 47 out of 108 positive cases (43.5%), while histopathological examination only detected 16 out of 108 cases (14.8%). From the followed-up subgroup 37 out of 106 patients (34.9%) experienced biochemical recurrence. It is noteworthy that qPCR yields more positive findings, regardless of the presence of biochemical recurrence.

Conclusion and clinical implications
The study findings illustrate that qPCR consistently outperforms conventional histology in detecting lymph node metastases, regardless of biochemical recurrence. The hypothesis that qPCR is better at predicting later biochemical recurrence than conventional histology has not been confirmed.
A new intelligent approach for frequency controller of autonomous hybrid power systems
Authors: Magdy Gaber , Bakeer Abualkasim , Bakeer Mahmoud , Albalawi Hani , Zaid Sherif A. 
Published: January 2025

AbstractAn intelligent approach for load frequency control based on a proportional-integral-derivative (PID) controller, referred to as an intelligent PID (IPID) controller, is proposed to enhance the frequency stability of autonomous hybrid power systems (HPSs) considering the high penetration levels of renewables. The proposed IPID is based on the ultra-local mod4el, i.e., a model-free control, which operates independently of the power system parameters since the controller incorporates a term for unknown disturbances, enhancing its control action. Moreover, a new meta-heuristic optimization algorithm, i.e., Geometric Mean Optimizer (GMO), is used to fine-tune the proposed IPID controller parameters for frequency regulation of the studied HPS. Furthermore, the superior performance of the proposed IPID controller based on the GMO is validated by comparing it with other counterpart controllers used; such as the PID and fractional-order PID (FOPID) controllers under different load/renewables perturbations, system uncertainties, and high renewables penetration. The Matlab simulation results demonstrate that the proposed IPID controller using GMO offers greater stability in reducing response deviations compared to traditional PID and FOPID controller structures. Where the proposed IPID controller demonstrated a 80% improvement in performance compared to the traditional PID controller, and a 57% improvement over the FOPID controller in handling scenarios with random load demand and high renewables penetration. Finally, to combine the accuracy of physical simulation and the adaptability of numerical simulation, the proposed IPID controller based on the GMO is verified and implemented in a real-time environment based on the PLECS RT Box platform.
Comprehensive histopathological analysis of gastric cancer in European and Latin America populations reveals differences in PDL1, HER2, p53 and MUC6 expression
Authors: Martínez-Ciarpaglini Carolina , Barros Rita , Caballero Carmelo , Boggino Hugo , Alarcón-Molero Lorena , Peleteiro Bárbara , Ruiz-García Erika , Fernandez-Figueroa Edith , Herrera-Goepfert Roberto , Díaz-Romero Consuelo , Ferreira Rui , Groen-van Schooten Tessa S. , Gauna Cinthia , Pereira Rita , Cantero Daniel , Lezcano Horacio , Esteso Federico , O´Connor Juan , Riquelme Arnoldo , Owen Gareth I. , Garrido Marcelo , Roa Juan Carlos , Ruiz-Pace Fiorella , Vivancos Ana , Diez-García Marc , Alsina Maria , Matito Judit , Martin Agatha , Gómez Marina , Castillo Ester , Vila Maria , Santos-Antunes João , Costa Andreia , Lordick Florian , Farrés Judith , Palomar-De Lucas Brenda , Cabeza-Segura Manuel , Villagrasa Rosanna , Jimenez-Martí Elena , Miralles-Marco Ana , Dienstmann Rodrigo , Derks Sarah , Figueiredo Ceu , Cervantes Andrés , Carneiro Fátima , Fleitas-Kanonnikoff Tania 
Published: January 2025

Abstract

Introduction
Gastric cancer (GC) burden is currently evolving with regional differences associated with complex behavioural, environmental, and genetic risk factors. The LEGACy study is a Horizon 2020-funded multi-institutional research project conducted prospectively to provide comprehensive data on the tumour biological characteristics of gastroesophageal cancer from European and LATAM countries.

Material and methods
Treatment-naïve advanced gastroesophageal adenocarcinoma patients were prospectively recruited in seven European and LATAM countries. Formalin-fixed paraffin-embedded primary tumour endoscopic biopsy samples were collected and submitted for central morphological and immunohistochemical characterization and TP53 molecular assessment and Helicobacter pylori infection.

Results
A total of 259 patients were included in the study: 137 (53%) from LATAM and 122 (47%) from Europe. Significant biological differences were detected between European and LATAM patients. Low representation of chromosomal instability (CIN) and HER2 positive cases were found in LATAM. MUC6 and PD-L1 were more frequently overexpressed in European cases, showing a significant correlation across the entire study population, with this association being especially pronounced in MMRdeficient cases. Both TP53 mutation by next-generation sequencing and p53 immunohistochemical aberrant pattern were linked with features associated with chromosomal instability. No regional differences were observed in H. pylori prevalence or abundance, indicating that the afore mentioned variations cannot be attributed to this factor.

Conclusion
Our findings underscore a need for region-specific approaches in gastroesophageal cancer diagnosis and treatment. MUC6 emerges as a putative immune regulator that needs further investigation. Research tailored to the unique biological profiles in different global regions is crucial to effectively address the observed disparities.