bluuebunny/crossref_metadata_2025_split huggingface dataset:
α-Synuclein Oligomers Induce the Loss of Dopaminergic Neurons via Mitochondrial Dysfunction and Oxidative Stress in an Intragastric Rotenone Mouse Model of Parkinson’s Disease
Authors: Huang Teng , Liang Qiming , Hong Mei , Huang Mengyang , Uversky Vladimir N , Pan-Montojo Francisco , Jiang Hong , Chen Juan , Zhou Honglian , Hu Qi 
Published: February 2022

Abstract
α-Synuclein (α-Syn) oligomers play a critical role in the pathogenesis of Parkinson's disease (PD). α-Syn oligomers inducing neuronal death are thought to be associated with the mitochondrial dysfunction and oxidative stress. A chronic PD mouse model was created by daily intragastric administration of rotenone (5 mg/kg, 12 weeks). We analysed the progression of α-Syn aggregates from the gut to the midbrain together with oxidative stress levels and mitochondrial morphology in neurons of the substantia nigra in these mice. Our results showed that α-Syn oligomers were accumulated in the midbrain of the wild-type mice sequentially. The number of dopaminergic neurons was significantly reduced in the rotenone-treated mice. These mice also showed increased reactive oxygen species levels and morphological alterations of mitochondria in the midbrain. These results were reproduced in the α-Syn-transfected SH-SY5Y cells. Collectively, these findings suggest that α-Syn oligomers induce neuronal death at least in part via mitochondrial dysfunction and oxidative stress.
Effect of liquid column on process stability and weld formation under ultra-high power fiber laser-arc hybrid welding of thick plates
Authors: Li Yan , Geng Shaoning , Gu Siyuan , Huang Dehua , Wang Yilin , Mi Gaoyang , Jiang Ping 
Published: February 2022

Abstract
Liquid column was a common problem in ultra-high power fiber laser-arc hybrid welding (UHLAHW) process, which has an adverse effect on the welding stability and weld formation. Hence, the influence of welding parameters on the behavior of liquid column were investigated systematically, including laser arc recombination process, laser power and welding speed. As a result, the violent rising liquid column under laser was suppressed markedly after arc addition. However, it was hardly to restrain liquid column upturn when laser power was extremely high, because the huge recoil pressure and shear force between metal vapor/plasma and molten pool could increase the upward momentum of melt. Besides, the height of liquid column and volume of spatters were directly influenced by welding speed, due to the welding line energy could significantly influence the flow of melt on the front of keyhole. By optimizing the welding process, the liquid column could be controlled and thus improved the stability. Finally, a sound weld bead with qualified weld formation and 15.82 mm penetration was produced when the laser power was 21 kW and welding speed was 1.2 m/min under UHLAHW process. This work provides technical guidance for achieving stable welding process and qualified weld formation for thick plates.
Long Non-Coding RNAs Roles in Tumorigenesis of Esophageal Carcinoma
Authors: Talebi Samaneh , Kargar Mohammad , jafarinia Mojtaba , Gholamin Mehran 
Published: February 2022

Abstract
Background:Esophageal carcinoma (ESCA) is one of the most common types of cancer. ESCA is accounted for the sixth leading cause of cancer-related deaths globally. The majority of the patients are diagnosed at late stages of ESCA, with distance metastasis and/or chemoresistance, which lead to a poor prognosis. Previous studies demonstrated lncRNA presentation and roles in ESCA cells and patients tissue. It has been proposed that lncRNAs can be considered as a new prognostic and diagnostic biomarker in ESCA. This study comprehensively explored the interaction of lncRNAs with miRNAs and mRNAs of TCGA database and proposed novel promising biomarker with favorable diagnostic and prognostic values. Methods: The public data of RNA-seq, miR-seq and related clinical data were downloaded from TCGA database. Differential expression analysis was conducted by “limma” in R. GO and KEGG signaling pathway were used for enrichments. STRING database was used for PPI analysis. CE-network was constructed by STAR database in R. Kaplan-Meier survival analysis (log-rank test) and ROC curve analysis to indicate the biomarkers' diagnostic and prognostic values.Results: Differentially expressed data illustrated that 1332 mRNA including 610 upregulated and 722 down-regulated were differentially expressed in ESCA.The GO and KEGG pathway analysis showed that the differentially expressed mRNAs were enriched in critical biological processes. The PPI showed that IGFBP5, ACAN, ADAMTS12, MMP13, and CDH2 were the important PPI hubs. The ceRNA network data demonstrated critical lncRNAs including TMEM16B-AS1, AC093010.3, SNHG3, and PVT1 which have an essential role in ESCA development.The data revealed that the lncRNA WDFY3-AS2, AC108449.2,DLEU2, AC007128.1, and AP003356.1 are potential diagnostic prognostic biomarker in the ESCA patients. Conclusion: Altogether, in our study, we demonstrated lncRNA, miRNA, and mRNA interaction and mentioned regulatory networks, which can be considered as a therapeutic option in ESCA. In addition, we proposed potential diagnostic and prognostic biomarkers for the patients.
The Association Between Triglyceride-glycemic Index and Obstructive Sleep Apnea (OSA): a Meta- Analysis
Authors: Wang Yuxiao , Liu Xiaoge , Xiang Yunan , Chen Bicheng , Chen Youli , Zhou Chaoyang 
Published: February 2022

Abstract
Background: Triglyceride-glycemic (TyG) index is proposed to be an alternative tool for evaluating insulin resistance (IR). In recent years, TyG index has emerged as a potential biomarker of obstructive sleep apnea (OSA), while its performance is still inconclusive. Therefore, we conducted a meta-analysis to evaluate the performance of TyG index in the diagnosis of OSA.Method: Systematic literature search through Pubmed, Web of Science, the Cochrane Library, Chinese National Knowledge Infrastructure (CNKI), and Wanfang data until November 17, 2021 was performed to retrieve studies that compared TyG index levels between OSA patients and healthy controls. The mean difference (MD) of TyG index was pooled using random-effect models. Results: Studies included in this meta-analysis uniformly identified increased levels of TyG index in OSA patients compared with control groups, and the pooled variance is 0.43 (95% CI = 0.36-0.50, P < 0.001; I² = 35%, p = 0.19). This finding is particularly reliable in severe OSA patients (MD = 0.39, 95% CI = 0.33-0.46, I² = 0%, p = 0.62). Conclusion: TyG index could be a promising biomarker for OSA, especially severe OSA with readily available and cost-effective evaluation techniques.
The role of silicon on C:N:P stoichiometry and yield of sugar beet under ammonium toxicity
Authors: Viciedo Dilier Olivera , Prado Renato de Mello , Peña Kolima , Calero-Hurtado Alexander , Piccolo Marisa de Cássia , Soares Mariana Bomfim , Lizcano Rodolfo Toledo 
Published: February 2022

Abstract
Most plants develop severe toxicity symptoms when grown on NH4+ as the sole nitrogen source, resulting in physiological and nutritional disturbances that slow vegetable growth. In this scenario of NH4+ stress, it is important to use strategies to mitigate this toxicity, and the use of silicon (Si) has promoted promising results. However, underlying studies on the effects of ammonia toxicity on C:N:P stoichiometric homeostasis and the role of Si in these stoichiometric ratios are lacking. In this study, we investigated the effects of different NH4+ concentration on the C:N:P stoichiometry and yield of sugar beet grown under hydroponic conditions, and examined whether the application of Si can alleviate the detrimental effects caused by toxic levels of NH4+. We conducted a hydroponic experiment to evaluate the effects of five concentrations of ammonium (1, 7.5, 15, 22.5, and 30 mmol L− 1) on C:N:P stoichiometry and yield of sugar beet cv. Early Wonder in the absence and presence (2 mmol L− 1) of Si. The experimental design was a randomized block design based on a 5 × 2 factorial scheme with four replicates. Our results indicate that in presence of Si both [N] and [P] in shoots and roots increased significantly and reaching its highest value at 15 mmol L− 1 of NH4+ in the nutrient solution, while shoot [C] remained stable, in roots increased with NH4+ concentrations of 22.5 and 30 mmol L− 1, respectively. In addition, shoot and root [Si] increased as higher levels of NH4+ in the nutrient solution. Our findings showed that homeostatic instability under highest levels of NH4+, especially in the plants cultivated without Si in the nutritive solution resulted in decreased shoots and roots dry matter production. In conclusion, both NH4+ concentration or Si in presence or absence in the nutrient solution can affect C:N:P stoichiometry of sugar beet.
Influence of different iron sources on Sb(III) removal from water by active iron-oxidizing bacteria and its mechanism
Authors: Li Yongchao , Liu Jialing , Mo Zhonggeng , Li Liyuan 
Published: February 2022

Abstract
Iron-oxidizing bacteria played an important role in the treatment of Sb-containing wastewater. In this study, effect of different iron sources on Sb(III) removal ability by isolated iron-oxidizing bacteria (named as IOB-L) was conducted systemically in batch experiment. Moreover, ferrous lactate and zero-valent iron were chosen as iron sources for IOB-L. The results showed that after inoculation of 2% volume of IOB-L, Sb(III) concentration in water decreased from initial 18 mg/L to 4.1 mg/L at optimal pH of 7.0. There was no reaction between Sb(III) and ferrous lactate, whereas corrosion product of iron can adsorb a certain amount of Sb. When active IOB-L cultivated in ferrous lactate, a better removal rate of Sb(III) can be reached with a longer stagnate phase for bacteria. However, Sb(III) removal ability of IOB-L using zero-valent iron as iron source was lower. SEM-EDS, FTIR, and XPS analysis further indicated that ferrous lactate was oxidized by IOB-L and precipitated as biogenic iron oxides which had strong adsorption ability towards Sb(III), whereas zero-valent iron was not a good iron source.
Elucidating the Role of Cardiac Biomarkers in COVID-19: A Narrative
Evaluation with Clinical Standpoints and a Pragmatic Approach for Therapeutics
Authors: Mukherjee Sukhes , Ray Suman Kumar , Kotnis Ashwin , Kanwar Jagat R 
Published: February 2022

Abstract:
With the incidence of the unabated spreading of the COVID-19 (coronavirus disease
2019) pandemic with an increase in heart-related complications in COVID-19 patients, laboratory
investigations on general health and diseases of heart have greater importance. The production of a
higher level of clots in the blood in COVID-19 individuals carries a high risk of severe lethal
pneumonia, pulmonary embolism, or widespread thromboembolism. The COVID-19 pandemic has
raised awareness regarding the severe consequences for the cardiac system that might cause due to
severe acute respiratory distress syndrome (SARS-CoV-2). COVID-19 causes acute respiratory
distress syndrome (ARDS), acute myocardial infarction, venous thromboembolism, and acute heart
failure in people with preexisting cardiac illness. However, as COVID-19 is primarily a respiratory
infectious disease, there is still a lot of debate on whether and how cardiac biomarkers should be
used in COVID-19 patients. Considering the most practical elucidation of cardiac biomarkers in
COVID-19, it is important to note that recent findings on the prognostic role of cardiac biomarkers
in COVID-19 patients are similar to those found in pneumonia and ARDS studies. The use of natriuretic
peptides and cardiac troponin concentrations as quantitative variables should help with
COVID-19/pneumonia risk classification and ensure that these biomarkers sustain their high diagnostic
precision for acute myocardial infarction and heart failure. Serial assessment of D-dimers
will possibly aid clinicians in the assortment of patients for venous thromboembolism imaging in
addition to the increase of anticoagulation from preventive to marginally higher or even therapeutic
dosages because of the central involvement of endothelitis and thromboembolism in COVID-19.
Therefore, cardiac biomarkers are produced in this phase because of some pathological processes;
this review will focus on major cardiac biomarkers and their significant role in COVID-19.
Synthesis and Characterization of Selenium Containing Schiff Bases: The
Selenosemicarbazones
Authors: Kshirsagar Anuraj S. , Khanna Pawan Kumar 
Published: February 2022

Background:
Many organoselenium compounds are well known for their applications in
various fields. However, some of the organoselenium compounds, like selenosemicarbazones, due to
difficulties in their synthesis are not much explored. Herein, we present the synthesis and characterization
of five different new selenosemicarbazones.

Objective:
The study aimed to carry out the synthesis and characterization of novel selenosemicarbazones,
a type of Schiff bases.

Methods:
Selenosemicarbazones are synthesized in a single step, i.e., acid-catalyzed condensation reaction
between ketones and hydrazine hydrate in the presence of potassium selenocyanate (KSeCN).

Results:
Butyrophenone, 4-phenyl 2-butanone, 2-acetonaphthone, 4-nitroacetophenone and menthone
were reacted with in-situ generated selenosemicarbazide, which led to the formation of respective selenosemicarbazones.
These organoselenium derivatives of Schiff bases have been characterized by
mass spectrometry, proton, carbon and selenium NMR.

Conclusion:
The yields of synthesized butyrophenone, 4-phenyl 2-butanone, 2-acetonaphthone, 4-
nitroacetophenone, and menthone selenosemicarbazones varied in between 44 to 65%. The synthesis
strategy involved one-pot synthesis of selenosemicarbazone without isolation of toxic selenosemicarbazide.
Long COVID Optimal Health Program (LC-OHP) to Enhance Psychological and Physical Health: Protocol for a Feasibility Randomized Controlled Trial
Authors: Al-Jabr Hiyam , Windle Karen , Thompson David R , Jenkins Zoe M , Castle David J , Ski Chantal F 
Published: February 2022

Background
Long COVID is a collection of symptoms that develop during or following a confirmed or suspected case of COVID-19, which continue for more than 12 weeks. Despite the negative impact of long COVID on people’s lives and functioning, there is no validated treatment or even rehabilitation guidance. What has been recommended thus far is the adoption of holistic management approaches. The Optimal Health Program (OHP) is a brief 5-session, plus booster, psychosocial program designed to support mental and physical well-being that has been used effectively for a range of chronic conditions.

Objective
This study examines the feasibility and acceptability of employing an especially customized version of OHP (long COVID OHP [LC-OHP]) to improve psychological and physical health of people with long COVID.

Methods
This is a feasibility randomized controlled trial that will be running from November 2021 to February 2023. Eligible participants aged 18 years or older who are experiencing symptoms of long COVID will be identified through their secondary practitioners with recruitment to be undertaken by the research team. A total of 60 participants will be randomized into a control (usual care) or an intervention (LC-OHP) group. Outcomes will be feasibility and acceptability of the program (primary); and efficacy of the LC-OHP in improving anxiety, depression, fatigue, self-efficacy, and quality of life (secondary). Up to 20 participants will be interviewed at the end of the trial to explore their experience with the program. Quantitative data will be analyzed using SPSS, and differences between groups will be compared using inferential tests where appropriate. Qualitative data will be transcribed and thematically analyzed to identify common emerging themes.

Results
This is an ongoing study, which began in November 2021.

Conclusions
Long COVID has a significant impact on an individual’s mental and physical functioning. The LC-OHP has a potential to provide people living with long COVID with additional support and to improve self-efficacy. The findings of this study would identify the feasibility of delivering this program to this population and will provide an indication for the program’s effectiveness.

Trial Registration
ISRCTN Registry ISRCTN38746119; https://www.isrctn.com/ISRCTN38746119

International Registered Report Identifier (IRRID)
DERR1-10.2196/36673
The effect of red cabbage extract to serum MDA levels in rats after maximum physical activity
Authors: Haris Nadia Husna , Nugroho Trilaksana , Utomo Astika Widy , Yora Nindita  
Published: February 2022

The maximum physical activity led to generate of free radical levels in the human body called oxidative stress. Red cabbage is well known as the vegetable containing highest anthocyanin among various other head cabbage plants. Anthocyanin is an antioxidant which is proven can prevent oxidative stress through reduction of MDA levels. The study aimed to investigate the effect of red cabbage extract (RCE) on MDA levels in rat after doing maximum physical activity. It was a true experimental study with post-test only control group design. Twenty-four male albino Wistar rats were used in this study. The rats were randomly divided into four groups i.e. the rats receiving maximum physical activity without and with RCE administration at dose of 86 mg/kg BW for eight days and the rats not receiving maximum physical activity without and with RCE administration. A swim test was used for maximum physical activity intervention. Serum MDA levels were measured using TBARS method. Data were analysed using one-way Anova followed by post-hoc LSD. A p value <0.005 was considered to be significant. The results showed that the rats treated with RCE both receiving maximum physical activity or not showed significant MDA reduction compared to rats without treated RCE (p=0.011 and p=0.027, respectively). Meanwhile, there was no significant difference in serum MDA levels between rats treated with RCE receiving maximum physical activity and rats not treated with RCE and not receiving maximum physical activity (p=0.540). Supplementation of RCE can reduce serum MDA levels in rats with and without maximum physical activity. Further study to investigate effective dose of RCE is recommended.
Estrategias didácticas en la enseñanza de los productos notables y la factorización en la telesecundaria
Authors: Gómez Segura Enrique 
Published: February 2022

El presente trabajo tiene el objetivo de resignificar las operaciones matemáticas de los productos notables y la factorización con procedimientos geométricos y razonamiento lógico matemático, de tal manera que los estudiantes vean las operaciones como inversas una de otra y esto les permita transitar matemáticamente con mayor facilidad. Este contenido matemático se estudia en el tercer grado de educación telesecundaria, en el primer bloque del programa. Para aplicar las actividades didácticas, se rescataron los conocimientos previos de los estudiantes, lo cual les permitió construir con mayor facilidad las reglas para el desarrollo de los productos notables como es el caso de los binomios cuadrados, conjugados y binomios con un término común; posteriormente, se realizaron las actividades inversas, es decir, pasar de un trinomio cuadrado perfecto a un binomio cuadrado y a sí sucesivamente con los otros dos productos notables. En cuanto a los resultados obtenidos por la implementación de las secuencias didácticas, 17 alumnos (85 %) tuvieron un mejor aprovechamiento escolar. En conclusión, los alumnos pudieron relacionar las operaciones en forma retrospectiva hasta llegar al punto de partida; este razonamiento casi desperdiciado en la enseñanza de la matemática permite apropiarse de la resolución de problemas con su respectiva comprobación, eso quiere decir que existe un aprendizaje con un razonamiento metacognitivo. Las únicas dificultades que se observaron en el aprendizaje se presentaron en la representación gráfica de los binomios conjugados y en la construcción de la regla de los binomios con un término común.
Telmisartan as an add-on treatment for dogs with refractory idiopathic epilepsy: a nonrandomized, uncontrolled, open-label clinical trial
Authors: Hanael Erez , Chai Orit , Konstanitin Lilach , Gibeon Laura , Rapaport Kira , Ruggeri Marco , Friedman Alon , Shamir Merav H. 
Published: February 2022

Abstract

OBJECTIVE
To evaluate the effect on seizure frequency of add-on telmisartan treatment in dogs with refractory idiopathic epilepsy.

ANIMALS
11 client-owned dogs with idiopathic epilepsy and ≥ 2 generalized seizures/mon that were currently being treated with ≥ 2 antiepileptic drugs.

PROCEDURES
Telmisartan was administered at a dosage of 0.25 to 1 mg/kg, PO, every 12 hours for 4 to 16 months. Seizure frequencies before and during telmisartan treatment were recorded.

RESULTS
10 dogs completed the 4-month treatment protocol. One dog was excluded owing to a transient increase in serum creatinine concentration; no adverse effects of telmisartan were observed in the remaining 10 dogs. A reduction in seizure frequency greater than an estimated expected placebo effect of 30% was evident in 7 of the 10 dogs. Long-term (12 to 16 months) follow-up information was available for 6 dogs, of which 4 had a further reduction in seizure frequency. Differences in seizure frequency were not statistically significant. No significant difference was found in serum phenobarbital concentration throughout the treatment period in the 7 dogs that were tested.

CLINICAL RELEVANCE
Telmisartan has the potential to reduce seizure frequency when administered as an add-on antiepileptic drug in dogs with refractory idiopathic epilepsy. A randomized, double-blind, placebo-controlled trial is needed to determine the true efficacy of telmisartan. On the basis of our results, a sample size of 54 dogs with refractory idiopathic epilepsy would be needed.
Prosopis spp. en enfermedades alérgicas
Authors: Velázquez-Sámano Guillermo , Mendoza-Gertrudis María de Lourdes , Velasco-Medina Andrea Aída , Rosas-Alvarado Alejandro 
Published: February 2022

Antecedentes: Actualmente, se entiende por polinosis, a la inflamación conjuntival y/o bronquial causada por alérgenos contenidos en los granos de polen ocasionando enfermedades como la rinoconjuntivitis y asma, todo ello con muy diversos grados de severidad. La sensibilización humana a prosopis se dio a conocer ya en la década de los 1950 gracias a estudios previos realizados por Ordman
Objetivos: demostrar la reactividad cutánea y la participación del polen de prosopis en los pacientes con enfermedades alérgicas que acuden al servicio de alergia e Inmunología clínica del Hospital General de México
Métodos: Se llevó a cabo un estudio retrospectivo y transversal analizando expedientes clínicos que contaran con historia clínica completa, entre 3-79 años de edad, con diagnósticos de rinitis, asma y rinoconjuntivitis alérgica.
Resultados: Se estudiaron 1285 pacientes, la frecuencia de reactividad cutánea positiva para prosopis fue de 27.78%, el 82.97% cursaba con diagnóstico de rinitis alérgica, 4.39% asma y 12.64% rinoconjuntivitis.
Conclusión: el polen de prosopis participa de manera importante en enfermedades de etiología alérgica. Es importante considerar el cruce antigénico con otras familias como las Chenopodiaceas.
Palabras clave: polinosis, prosopis, reactividad cruzada, aerobiología.