bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metasurface-driven full-space structured light for three-dimensional imaging
Authors: Kim Gyeongtae , Kim Yeseul , Yun Jooyeong , Moon Seong-Won , Kim Seokwoo , Kim Jaekyung , Park Junkyeong , Badloe Trevon , Kim Inki , Rho Junsuk 
Published: October 2022

AbstractStructured light (SL)-based depth-sensing technology illuminates the objects with an array of dots, and backscattered light is monitored to extract three-dimensional information. Conventionally, diffractive optical elements have been used to form laser dot array, however, the field-of-view (FOV) and diffraction efficiency are limited due to their micron-scale pixel size. Here, we propose a metasurface-enhanced SL-based depth-sensing platform that scatters high-density ~10 K dot array over the 180° FOV by manipulating light at subwavelength-scale. As a proof-of-concept, we place face masks one on the beam axis and the other 50° apart from axis within distance of 1 m and estimate the depth information using a stereo matching algorithm. Furthermore, we demonstrate the replication of the metasurface using the nanoparticle-embedded-resin (nano-PER) imprinting method which enables high-throughput manufacturing of the metasurfaces on any arbitrary substrates. Such a full-space diffractive metasurface may afford ultra-compact depth perception platform for face recognition and automotive robot vision applications.
Direct strain correlations at the single-atom level in three-dimensional core-shell interface structures
Authors: Jo Hyesung , Wi Dae Han , Lee Taegu , Kwon Yongmin , Jeong Chaehwa , Lee Juhyeok , Baik Hionsuck , Pattison Alexander J. , Theis Wolfgang , Ophus Colin , Ercius Peter , Lee Yea-Lee , Ryu Seunghwa , Han Sang Woo , Yang Yongsoo 
Published: October 2022

AbstractNanomaterials with core-shell architectures are prominent examples of strain-engineered materials. The lattice mismatch between the core and shell materials can cause strong interface strain, which affects the surface structures. Therefore, surface functional properties such as catalytic activities can be designed by fine-tuning the misfit strain at the interface. To precisely control the core-shell effect, it is essential to understand how the surface and interface strains are related at the atomic scale. Here, we elucidate the surface-interface strain relations by determining the full 3D atomic structure of Pd@Pt core-shell nanoparticles at the single-atom level via atomic electron tomography. Full 3D displacement fields and strain profiles of core-shell nanoparticles were obtained, which revealed a direct correlation between the surface and interface strain. The strain distributions show a strong shape-dependent anisotropy, whose nature was further corroborated by molecular statics simulations. From the observed surface strains, the surface oxygen reduction reaction activities were predicted. These findings give a deep understanding of structure-property relationships in strain-engineerable core-shell systems, which can lead to direct control over the resulting catalytic properties.
The influence of alloying on slip intermittency and the implications for dwell fatigue in titanium
Authors: Worsnop Felicity F. , Lim Rachel E. , Bernier Joel V. , Pagan Darren C. , Xu Yilun , McAuliffe Thomas P. , Rugg David , Dye David 
Published: October 2022

AbstractDwell fatigue, the reduction in fatigue life experienced by titanium alloys due to holds at stresses as low as 60% of yield, has been implicated in several uncontained jet engine failures. Dislocation slip has long been observed to be an intermittent, scale-bridging phenomenon, similar to that seen in earthquakes but at the nanoscale, leading to the speculation that large stress bursts might promote the initial opening of a crack. Here we observe such stress bursts at the scale of individual grains in situ, using high energy X-ray diffraction microscopy in Ti–7Al–O alloys. This shows that the detrimental effect of precipitation of ordered Ti3Al is to increase the magnitude of rare pri〈a〉 and bas〈a〉 slip bursts associated with slip localisation. By contrast, the addition of trace O interstitials is beneficial, reducing the magnitude of slip bursts and promoting a higher frequency of smaller events. This is further evidence that the formation of long paths for easy basal plane slip localisation should be avoided when engineering titanium alloys against dwell fatigue.
Cholesterol-stabilized membrane-active nanopores with anticancer activities
Authors: Shen Jie , Gu Yongting , Ke Lingjie , Zhang Qiuping , Cao Yin , Lin Yuchao , Wu Zhen , Wu Caisheng , Mu Yuguang , Wu Yun-Long , Ren Changliang , Zeng Huaqiang 
Published: October 2022

AbstractCholesterol-enhanced pore formation is one evolutionary means cholesterol-free bacterial cells utilize to specifically target cholesterol-rich eukaryotic cells, thus escaping the toxicity these membrane-lytic pores might have brought onto themselves. Here, we present a class of artificial cholesterol-dependent nanopores, manifesting nanopore formation sensitivity, up-regulated by cholesterol of up to 50 mol% (relative to the lipid molecules). The high modularity in the amphiphilic molecular backbone enables a facile tuning of pore size and consequently channel activity. Possessing a nano-sized cavity of ~ 1.6 nm in diameter, our most active channel Ch-C1 can transport nanometer-sized molecules as large as 5(6)-carboxyfluorescein and display potent anticancer activity (IC50 = 3.8 µM) toward human hepatocellular carcinomas, with high selectivity index values of 12.5 and >130 against normal human liver and kidney cells, respectively.
Topological current divider in a Chern insulator junction
Authors: Ovchinnikov Dmitry , Cai Jiaqi , Lin Zhong , Fei Zaiyao , Liu Zhaoyu , Cui Yong-Tao , Cobden David H. , Chu Jiun-Haw , Chang Cui-Zu , Xiao Di , Yan Jiaqiang , Xu Xiaodong 
Published: October 2022

AbstractA Chern insulator is a two-dimensional material that hosts chiral edge states produced by the combination of topology with time reversal symmetry breaking. Such edge states are perfect one-dimensional conductors, which may exist not only on sample edges, but on any boundary between two materials with distinct topological invariants (or Chern numbers). Engineering of such interfaces is highly desirable due to emerging opportunities of using topological edge states for energy-efficient information transmission. Here, we report a chiral edge-current divider based on Chern insulator junctions formed within the layered topological magnet MnBi2Te4. We find that in a device containing a boundary between regions of different thickness, topological domains with different Chern numbers can coexist. At the domain boundary, a Chern insulator junction forms, where we identify a chiral edge mode along the junction interface. We use this to construct topological circuits in which the chiral edge current can be split, rerouted, or switched off by controlling the Chern numbers of the individual domains. Our results demonstrate MnBi2Te4 as an emerging platform for topological circuits design.
Direct observation of ultrafast singlet exciton fission in three dimensions
Authors: Ashoka Arjun , Gauriot Nicolas , Girija Aswathy V. , Sawhney Nipun , Sneyd Alexander J. , Watanabe Kenji , Taniguchi Takashi , Sung Jooyoung , Schnedermann Christoph , Rao Akshay 
Published: October 2022

AbstractWe present quantitative ultrafast interferometric pump-probe microscopy capable of tracking of photoexcitations with sub-10 nm spatial precision in three dimensions with 15 fs temporal resolution, through retrieval of the full transient photoinduced complex refractive index. We use this methodology to study the spatiotemporal dynamics of the quantum coherent photophysical process of ultrafast singlet exciton fission. Measurements on microcrystalline pentacene films grown on glass (SiO2) and boron nitride (hBN) reveal a 25 nm, 70 fs expansion of the joint-density-of-states along the crystal a,c-axes accompanied by a 6 nm, 115 fs change in the exciton density along the crystal b-axis. We propose that photogenerated singlet excitons expand along the direction of maximal orbital π-overlap in the crystal a,c-plane to form correlated triplet pairs, which subsequently electronically decouples into free triplets along the crystal b-axis due to molecular sliding motion of neighbouring pentacene molecules. Our methodology lays the foundation for the study of three dimensional transport on ultrafast timescales.
The E3 ubiquitin ligase ARIH1 promotes antiviral immunity and autoimmunity by inducing mono-ISGylation and oligomerization of cGAS
Authors: Xiong Tian-Chen , Wei Ming-Cong , Li Fang-Xu , Shi Miao , Gan Hu , Tang Zhen , Dong Hong-Peng , Liuyu Tianzi , Gao Pu , Zhong Bo , Zhang Zhi-Dong , Lin Dandan 
Published: October 2022

AbstractThe cytosolic DNA sensor cyclic GMP-AMP synthase (cGAS) plays a critical role in antiviral immunity and autoimmunity. The activity and stability of cGAS are fine-tuned by post-translational modifications. Here, we show that ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1) catalyzes the mono-ISGylation and induces the oligomerization of cGAS, thereby promoting antiviral immunity and autoimmunity. Knockdown or knockout of ARIH1 significantly inhibits herpes simplex virus 1 (HSV-1)- or cytoplasmic DNA-induced expression of type I interferons (IFNs) and proinflammatory cytokines. Consistently, tamoxifen-treated ER-Cre;Arih1fl/fl mice and Lyz2-Cre; Arih1fl/fl mice are hypersensitive to HSV-1 infection compared with the controls. In addition, deletion of ARIH1 in myeloid cells alleviates the autoimmune phenotypes and completely rescues the autoimmune lethality caused by TREX1 deficiency. Mechanistically, HSV-1- or cytosolic DNA-induced oligomerization and activation of cGAS are potentiated by ISGylation at its K187 residue, which is catalyzed by ARIH1. Our findings thus reveal an important role of ARIH1 in innate antiviral and autoimmune responses and provide insight into the post-translational regulation of cGAS.
A proteome-scale map of the SARS-CoV-2–human contactome
Authors: Kim Dae-Kyum , Weller Benjamin , Lin Chung-Wen , Sheykhkarimli Dayag , Knapp Jennifer J. , Dugied Guillaume , Zanzoni Andreas , Pons Carles , Tofaute Marie J. , Maseko Sibusiso B. , Spirohn Kerstin , Laval Florent , Lambourne Luke , Kishore Nishka , Rayhan Ashyad , Sauer Mayra , Young Veronika , Halder Hridi , la Rosa Nora Marín-de , Pogoutse Oxana , Strobel Alexandra , Schwehn Patrick , Li Roujia , Rothballer Simin T. , Altmann Melina , Cassonnet Patricia , Coté Atina G. , Vergara Lena Elorduy , Hazelwood Isaiah , Liu Betty B. , Nguyen Maria , Pandiarajan Ramakrishnan , Dohai Bushra , Coloma Patricia A. Rodriguez , Poirson Juline , Giuliana Paolo , Willems Luc , Taipale Mikko , Jacob Yves , Hao Tong , Hill David E. , Brun Christine , Twizere Jean-Claude , Krappmann Daniel , Heinig Matthias , Falter Claudia , Aloy Patrick , Demeret Caroline , Vidal Marc , Calderwood Michael A. , Roth Frederick P. , Falter-Braun Pascal 
Published: October 2022

AbstractUnderstanding the mechanisms of coronavirus disease 2019 (COVID-19) disease severity to efficiently design therapies for emerging virus variants remains an urgent challenge of the ongoing pandemic. Infection and immune reactions are mediated by direct contacts between viral molecules and the host proteome, and the vast majority of these virus–host contacts (the ‘contactome’) have not been identified. Here, we present a systematic contactome map of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with the human host encompassing more than 200 binary virus–host and intraviral protein–protein interactions. We find that host proteins genetically associated with comorbidities of severe illness and long COVID are enriched in SARS-CoV-2 targeted network communities. Evaluating contactome-derived hypotheses, we demonstrate that viral NSP14 activates nuclear factor κB (NF-κB)-dependent transcription, even in the presence of cytokine signaling. Moreover, for several tested host proteins, genetic knock-down substantially reduces viral replication. Additionally, we show for USP25 that this effect is phenocopied by the small-molecule inhibitor AZ1. Our results connect viral proteins to human genetic architecture for COVID-19 severity and offer potential therapeutic targets.
Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencing
Authors: Kishi Jocelyn Y. , Liu Ninning , West Emma R. , Sheng Kuanwei , Jordanides Jack J. , Serrata Matthew , Cepko Constance L. , Saka Sinem K. , Yin Peng 
Published: October 2022

AbstractWe present Light-Seq, an approach for multiplexed spatial indexing of intact biological samples using light-directed DNA barcoding in fixed cells and tissues followed by ex situ sequencing. Light-Seq combines spatially targeted, rapid photocrosslinking of DNA barcodes onto complementary DNAs in situ with a one-step DNA stitching reaction to create pooled, spatially indexed sequencing libraries. This light-directed barcoding enables in situ selection of multiple cell populations in intact fixed tissue samples for full-transcriptome sequencing based on location, morphology or protein stains, without cellular dissociation. Applying Light-Seq to mouse retinal sections, we recovered thousands of differentially enriched transcripts from three cellular layers and discovered biomarkers for a very rare neuronal subtype, dopaminergic amacrine cells, from only four to eight individual cells per section. Light-Seq provides an accessible workflow to combine in situ imaging and protein staining with next generation sequencing of the same cells, leaving the sample intact for further analysis post-sequencing.
Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model
Authors: Maeda Shusaku , Kawamura Takuji , Sasaki Masanori , Shimamura Kazuo , Shibuya Takashi , Harada Akima , Honmou Osamu , Sawa Yoshiki , Miyagawa Shigeru 
Published: October 2022

AbstractIntravenous infusion of stem cells is a minimally invasive cellular delivery method, though a few have been reported in a critical limb-threatening ischemia (CLTI) animal model or patients. In the present study, we hypothesized that intravenous infusion of bone-marrow derived mesenchymal stem cells (MSCs) improves tissue perfusion in a rat hindlimb ischemia model. Hindlimb ischemia was generated in Sprague–Dawley rats by femoral artery removal, then seven days after ischemic induction intravenous infusion of 1 × 106MSCs (cell group) or vehicle (control group) was performed. As compared with the control, tissue perfusion was significantly increased in the cell group. Histological findings showed that capillary density was significantly increased in the cell group, with infused green fluorescent protein (GFP)-MSCs distributed in the ischemic limb. Furthermore, gene expression of vascular endothelial growth factor (VEGF) was significantly increased in ischemic hindlimb muscle tissues of rats treated with MSC infusion. In conclusion, intravenous infusion of bone-marrow derived MSCs improved tissue perfusion in ischemic hindlimbs through angiogenesis, suggesting that intravenous infusion of MSCs was a promising cell delivery method for treatment of CLTI.
Spatio-temporal metabolic rewiring in the brain of TgF344-AD rat model of Alzheimer’s disease
Authors: Muñoz-Moreno Emma , Simões Rui Vasco , Tudela Raúl , López-Gil Xavier , Soria Guadalupe 
Published: October 2022

AbstractBrain damage associated with Alzheimer's disease (AD) occurs even decades before the symptomatic onset, raising the need to investigate its progression from prodromal stages. In this context, animal models that progressively display AD pathological hallmarks (e.g. TgF344-AD) become crucial. Translational technologies, such as magnetic resonance spectroscopy (MRS), enable the longitudinal metabolic characterization of this disease. However, an integrative approach is required to unravel the complex metabolic changes underlying AD progression, from early to advanced stages. TgF344-AD and wild-type (WT) rats were studied in vivo on a 7 Tesla MRI scanner, for longitudinal quantitative assessment of brain metabolic profile changes using MRS. Disease progression was investigated at 4 time points, from 9 to 18 months of age, and in 4 regions: cortex, hippocampus, striatum, and thalamus. Compared to WT, TgF344-AD rats replicated common findings in AD patients, including decreased N-acetylaspartate in the cortex, hippocampus and thalamus, and decreased glutamate in the thalamus and striatum. Different longitudinal evolution of metabolic concentration was observed between TgF344-AD and WT groups. Namely, age-dependent trajectories differed between groups for creatine in the cortex and thalamus and for taurine in cortex, with significant decreases in Tg344-AD animals; whereas myo-inositol in the thalamus and striatum showed greater increase along time in the WT group. Additional analysis revealed divergent intra- and inter-regional metabolic coupling in each group. Thus, in cortex, strong couplings of N-acetylaspartate and creatine with myo-inositol in WT, but with taurine in TgF344-AD rats were observed; whereas in the hippocampus, myo-inositol, taurine and choline compounds levels were highly correlated in WT but not in TgF344-AD animals. Furthermore, specific cortex-hippocampus-striatum metabolic crosstalks were found for taurine levels in the WT group but for myo-inositol levels in the TgF344-AD rats. With a systems biology perspective of metabolic changes in AD pathology, our results shed light into the complex spatio-temporal metabolic rewiring in this disease, reported here for the first time. Age- and tissue-dependent imbalances between myo-inositol, taurine and other metabolites, such as creatine, unveil their role in disease progression, while pointing to the inadequacy of the latter as an internal reference for quantification.
Effect of ivabradine on cognitive functions of rats with scopolamine-induced dementia
Authors: Assi Abdel-Azim , Abdelnabi Sara , Attaai Abdelraheim , Abd-ellatief Rasha B. 
Published: October 2022

AbstractAlzheimer’s disease is among the challenging diseases to social and healthcare systems because no treatment has been achieved yet. Although the ambiguous pathological mechanism underlying this disorder, ion channel dysfunction is one of the recently accepted possible mechanism. Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels play important roles in cellular excitability and synaptic transmission. Ivabradine (Iva), an HCN blocker, is acting on HCN channels, and is clinically used for angina and arrhythmia. The current study aimed to investigate the therapeutic effects of Iva against scopolamine (Sco) induced dementia. To test our hypothesis, Sco and Iva injected rats were tested for behavioural changes, followed by ELISA and histopathological analysis of the hippocampus. Induced dementia was confirmed by behavioural tests, inflammatory cytokines and oxidative stress tests and histopathological signs of neurodegeneration, multifocal deposition of congo red stained amyloid beta plaques and the decreased optical density of HCN1 immunoreactivity. Iva ameliorated the scopolamine-induced dysfunction, the hippocampus restored its normal healthy neurons, the amyloid plaques disappeared and the optical density of HCN1 immunoreactivity increased in hippocampal cells. The results suggested that blockage of HCN1 channels might underly the Iva therapeutic effect. Therefore, Iva might have beneficial effects on neurological disorders linked to HCN channelopathies.
A numerical approach for preventing the dispersion of infectious disease in a meeting room
Authors: Ahmadzadeh Mahdi , Shams Mehrzad 
Published: October 2022

AbstractAirborne transmission of respiratory aerosols carrying infectious viruses has generated many concerns about cross-contamination risks, particularly in indoor environments. ANSYS Fluent software has been used to investigate the dispersion of the viral particles generated during a coughing event and their transport dynamics inside a safe social-distance meeting room. Computational fluid dynamics based on coupled Eulerian–Lagrangian techniques are used to explore the characteristics of the airflow field in the domain. The main objective of this study is to investigate the effects of the window opening frequency, exhaust layouts, and the location of the air conditioner systems on the dispersion of the particles. The results show that reducing the output capacity by raising the concentration of suspended particles and increasing their traveled distance caused a growth in the individuals' exposure to contaminants. Moreover, decreasing the distance between the ventilation systems installed location and the ceiling can drop the fraction of the suspended particles by over 35%, and the number of individuals who are subjected to becoming infected by viral particles drops from 6 to 2. As well, the results demonstrated when the direction of input airflow and generated particles were the same, the fraction of suspended particles of 4.125%, whereas if the inputs were shifted to the opposite direction of particle injection, the fraction of particles in fluid increased by 5.000%.
Active tactile sensing of small insect force by a soft microfinger toward microfinger-insect interactions
Authors: Konishi Satoshi , Mori Fuminari , Kakehi Yugo , Shimizu Ayano , Sano Fumiya , Koyanagi Kodai 
Published: October 2022

AbstractHuman–robot interaction technology has contributed to improving sociality for humanoid robots. At scales far from human scales, a microrobot can interact with an environment in a small world. Microsensors have been applied to measurement of forces by flying or walking insects. Meanwhile, most previous works focused on the measurement of the behavior of insects. Here, we propose microrobot-insect interactions by soft microfingers integrated with artificial muscle actuators and tactile sensors, which has been developed for a haptic teleoperation robot system. A soft pneumatic balloon actuator acts as the artificial muscle, and a flexible strain sensor using a liquid metal provides tactile sensing. Force interaction between a pill bug and the microfinger could be accomplished. The microfinger (12 mm × 3 mm × 490 μm) can move and touch an insect, and it can detect reaction force from an insect. The measured reaction force from the legs of a pill bug as a representative insect was less than 10 mN. This paper presents a microfinger as an end effector for the active sensing of reaction force from a small insect. We anticipate that our results will lead to further evaluation of small living things as well as technology development for human–environment interaction.