bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of magnesium oxide nanocomposite film made from sugarcane bagasse waste
Authors: Kumar Harish , Gaur Ankur , Karuna M. S. 
Published: November 2022

Abstract
In this study, hydrogels comprised of magnesium oxide nanoparticles (MgO-NPs) are synthesized in situ and employed to make films from biopolymer nanocomposite materials. Synthesis of CMC from various types of wastes mainly depends on the whole pre-treatment process. UV-Vis spectroscopy, Fourier transmission electron microscopy (FTIR), scanning electron microscopy (SEM), Energy dispersive X-ray (EDX), and transform infrared spectroscopy were used to characterize MgO-NPs. A TEM image reveals MgO-NPs of 100 nm embedded in the sugarcane bagasse carboxymethyl cellulose (SB-CMC) hydrogel network. The successful absorption peak at 295 nm, which is brought on by the surface plasmon resonance (SPR), indicates the existence of MgO-NPs. A film with nanoparticles (SB-CMC-2) demonstrated a slower rate of biodegradation than the Film without nanoparticles (SB-CMC-1) sample. Glycerol typically increases film flexibility and extension. Film tensile strength (TS) and opacity are increased by MgO-NPs in CMC film while moisture content, solubility, and elongation at break are decreased.
Subquadratic-time algorithm for the diameter and all eccentricities on median graphs
Authors: Bergé Pierre , Ducoffe Guillaume , Habib Michel 
Published: November 2022

Abstract
On sparse graphs, Roditty and Williams [2013] proved that no O(n2−ε)- time algorithm achieves an approximation factor smaller than 3/2 for the diameter problem unless SETH fails. In this article, we solve an open question formulated in the literature: can we use the structural properties of median graphs to break this global quadratic barrier? We propose the first combinatiorial algorithm computing exactly all eccentricities of a median graph in truly subquadratic time. Median graphs constitute the family of graphs which is the most studied in metric graph theory because their structure represents many other discrete and geometric concepts, such as CAT(0) cube complexes. Our result generalizes a recent one, stating that there is a linear-time algorithm for all eccentricities in median graphs with bounded dimension d, i.e. the dimension of the largest induced hypercube. This prerequisite on d is not necessarily anymore to determine all eccentricities in subquadratic time. The execution time of our algorithm is O(n1.6456 logO(1) n). We provide also some satellite outcomes related to this general result. In particular, restricted to simplex graphs, this algorithm enumerates all eccentricities with a quasilinear running time. Moreover, an algorithm is proposed to compute exactly all reach centralities in time O(23dn logO(1) n).
Neonatal Outcome in Pregnant Women with Confirmed COVID-19 Infection during the Last Two Weeks of a Viable Pregnancy: A Retrospective Data Analysis
Authors: Salameh Khalil Mohd Khalil , Valappil Rajesh Pattu , Vellamgot Anvar Paraparambil , Abedin Sarfrazul , Elkabir Naser Abulgasim , Elhaji Esam Mohamed Elhadi , Habboub Lina Hussain M. , Alhoyed Samer Mahmoud 
Published: November 2022

Abstract
Background: Pregnant women are typically vulnerable to infectious diseases due to exaggerated disease manifestations and detrimental effects on their obstetric and neonatal outcomes. Previous studies have demonstrated that pregnant women with COVID-19 had similar clinical manifestations as nonpregnant women. However, there is not enough knowledge about the outcomes of neonates born to infected women although it has been reported that maternal pneumonia results in unfavorable obstetrical outcomes, including the premature rupture of membranes (PROM), intrauterine fetal demise (IUFD), intrauterine growth restriction (IUGR), and neonatal death. This retrospective study analyzes the clinical characteristics of all women with confirmed COVID-19 infection who gave birth in AWH, Qatar, as well as the possible adverse neonatal outcomes associated with maternal COVID-19 infection.
Objective: To identify adverse neonatal outcomes in mothers with confirmed COVID-19 infection during the last two weeks of a viable pregnancy.
Methods: This retrospective study included newborn babies born to mothers diagnosed with COVID-19 infection between 1 April 2021 and 23 May 2021 at AWH, Hamad Medical Corporation.
Data: Clinical characteristics, investigation results, and course of treatment were gathered from medical records for both mothers and babies.
Results: Out of 108 babies born to COVID-19-infected mothers, 47 (43.5%) were identified with adverse neonatal outcomes. Prematurity (28.7%), low birth weight (26%), respiratory distress (33.3%), and neonatal depression (8.3%) were the most commonly associated outcomes. Eight out of 108 babies (7.4%) tested positive for COVID-19, with 4.6% incidence of vertical transmission and 2.8% transient viremia. Using logistic regression analysis, maternal pneumonia and CT values were found to be statistically significant factors for premature delivery but were not significantly associated with neonatal infection. However, maternal ferritin levels significantly predicted neonatal positive PCR results.
Conclusion: Our data support the possibility of the intrauterine transmission of SARS-CoV-2 even in asymptomatic women. Studies with a larger number of subjects are recommended for identifying the biological mechanisms involved.
Novel soil-less potting mixes for the mycorrhization of Quercus pubescens Willd. seedlings with Tuber melanosporum Vittad
Authors: Pagliarani Sofia , Vannini Andrea , Kuzminsky Elena , Morales-Rodríguez Carmen 
Published: November 2022

Abstract
Truffle cultivation has quickly grown in European countries and elsewhere, so optimization of the nursery inoculation method to cover the high demand for mycorrhized seedlings is needed. The suitability of two compost-based potting mixes to produce Quercus pubescens Willd. mycorrhized with the black Périgord truffle T. melanosporum Vittad. was tested as an alternative to the employ of traditional potting mix used for mycorrhization and considered as the control treatment. The effects on the mycorrhizal development and the morphometric assessment of the root and shoot system of the downy oak seedlings were investigated 8 months after the spore slurry inoculation in a glasshouse experiment using the root analysis software WinRHIZO®. From the results obtained, the compost mix containing green organic residues from pruning and mowing (Mix 2) achieved better performance with a higher mycorrhization percentage than the control (59.7% and 46.7%, respectively) and significantly higher root growth with enhanced root length (531 cm), number of root tips (3.329), and root forks (3.941) both with respect to the control and the potting mix containing municipal and green organic residues (Mix 1). In conclusion, from this work is evident that potting mix containing recycled organic matter, which is readily available, cheap, and environmentally sustainable can offer excellent mycorrhization performances and may be included in the mycorrhization process of Q. pubescens seedlings with T. melanosporum under controlled conditions.
Single inferior parathyroid autotransplantation during total thyroidectomy with bilateral central lymph node dissection for papillary thyroid carcinoma: a retrospective cohort study
Authors: Dong Zhizhong , Liu Wen , Peng Ying , Zhan Xiangxiang , Su Yanjun , Diao Chang , Cheng Ruochuan 
Published: November 2022

Abstract
Background: Management of the inferior parathyroid gland using total thyroidectomy (TT) with central lymph node dissection (CLND) is still controversial. Therefore, we evaluated the safety and effectiveness of single inferior parathyroid autotransplantation.
Methods: The clinical data of patients with papillary thyroid carcinoma (PTC) who underwent TT with bilateral CLND from January 2018 to December 2020 were collected. Quality of life (QOL) was assessed using the Chinese version of the EORTC QLQ-C30 and THYCA-QOL. The patients were divided into an autotransplantation group and a preservation group according to whether a single inferior parathyroid gland was transplanted. The incidence of permanent hypoparathyroidism, the number of resected central lymph nodes (CLNs), the rate of recurrence reoperation, the rate of radioactive iodine (RAI) treatment, and the quality of life (QOL) score were compared between the two groups.
Results: A total of 296 patients were included in the study, there were 99 patients in the autotransplantation group and 197 in the preservation group. The incidence of permanent hypoparathyroidism was 3.0% (3/99) and 4.6% (9/197) in the autotransplantation and preservation groups, respectively (P = 0.532). The median number of resected CLNs was 12 (8-17) and 10 (6-14) in the autotransplantation and preservation groups, respectively (P = 0.015). No reoperations were performed for patients with CLN recurrence, and the rates of lateral lymph node (LLN) recurrence reoperation were 2.0% (2/99) and 3.6% (7/197) in the autotransplantation and preservation groups, respectively (P = 0.473). The RAI treatment rates were 12.1% (12/99) and 22.3% (44/197) in the autotransplantation and preservation groups, respectively (P = 0.034). A total of 276 questionnaires were recovered, including 84 in the autotransplantation group and 192 in the preservation group. The QOL of the two groups of patients is similar (P > 0.05).
Conclusion: Single inferior parathyroid autotransplantation during thyroidectomy can be used to prevent permanent hypoparathyroidism and can remove more CLNs.
Comprehensive Review for Healthcare Data Quality Challenges in Blockchain Technology
Authors: Halimeh Ahmed Abu , Ali Omar 
Published: November 2022

Abstract
There are several features inherent in blockchain, including decentralized storage, distributed ledger, immutability, security and authentication, and it has shifted away from the hype to be used practically in different industries, such as in the healthcare sector. The use of blockchain technology has allowed the provision of improved services to industries. The objective of this paper is to demonstrate how the use of blockchain is influenced by data quality issues in the healthcare industry. The article is structured as a systematic literature review study that uses several articles issued in various databases from 2016 onwards. In this review study, 65 articles were chosen and grouped into a single key aspect of the challenge in the healthcare sector. The findings obtained were analyzed based on factors in three domains, classified as issues pertinent to the adoption, operational and technological domains. This review study aims to use the findings to provide support to the practitioners, stakeholders and professionals, whose purpose is to carry out and manage transformation projects pertinent to blockchain in the field of healthcare. In addition, the organizations would be facilitated in their decision-making processes when the potential blockchain users are made to comprehend the implicit factors related to blockchain.
Revising primary data for conservation status assessments of Brazilian threatened butterflies
Authors: Rosa Augusto Henrique Batista , Ribeiro Danilo Bandini , Freitas André Victor Lucci 
Published: November 2022

Abstract
Primary data, such as geographical records of species, are base-line for conservation status assessments. For many years, data on Brazil’s threatened butterflies (58 taxa) have been in need of improvement and the present paper provides a careful review of their geographical distribution data as well as an update of extent of occurrence (EOO) and area of occupancy (AOO). In total, ~ 6,700 records were compiled from several sources; 1,053 records are non duplicated geographical data. Of these 1,053 records, 566 (69%) come from surveyed literature (published records), 258 (31%) are unpublished records, and 229 (22%) were found to contain errors after data curation. Comparing “previous” to “current” estimates of both, EOO and AOO, changes in geographical range were reported for 48 taxa (83%). Based on current data (applying the thresholds of criterion B), there is a potential for changes in conservation status categories for 51 taxa (88%). Importantly, approximately half of unpublished records are from scientific collections and the remainder were provided by civil society (via personal communication), showing the importance of both data sources. The present updates of geographical records (and consequently, EOO and AOO) of Brazilian threatened butterflies may aid future conservation status assessments and also reinforce the importance of data curation.
Neoadjuvant Vidutolimod and Nivolumab in High-Risk Resectable Melanoma
Authors: Davar Diwakar , Karunamurthy Arivarasan , Chauvin Joe-Marc , Morrison Robert , Bai Yulong , Sun Jie , Stein Julie , Rodrigues Richard , McCulloch John , Wang Hong , Hartman Douglas , Fernandes Miriam , Cole Alicia , Dzutsev Amiran , Deitrick Christopher , Bao Riyue , Duvvuri Umamaheswar , Sridha Shaum , Kim Seungwon , Choudry M. Haroon , Holtzman Matthew , Pingpank James , O'Toole James , DeBlasio Richelle , Jin Yang , Ding Quanquan , Gao Wentao , Groetsch Christopher , Pagliano Ornella , Rose Amy , Urban Corey , Singh Jagjit , Divakar Prajan , Mauro David , Bobilev Dmitri , Wooldridge James , Krieg Arthur , Fury Matthew , Najjar Yana , Luke Jason , Kirkwood John , Taube Janis , Park Hyun Jung , Trinchieri Giorgio , Zarour Hassane 
Published: November 2022

Abstract

Intratumoral TLR9 agonists and anti-PD-1 provide durable clinical responses and broad immune activation. To evaluate efficacy and mechanisms of action of this combined treatment, we conducted a single-arm phase 2 neoadjuvant study of TLR9 agonist vidutolimod combined with anti-PD-1 nivolumab (vidu/nivo) in patients with high-risk resectable melanoma. In 30 evaluable patients, 57% (17/30) had major pathologic response (pR), with eight grade 3 treatment-related adverse events. pR was associated with lower residual viable tumor, necrosis, and melanophagocytosis with increased CD8+ tumor-infiltrating lymphocytes and plasmacytoid dendritic cells (pDCs) in the tumor microenvironment and increased frequencies of Ki67+ CD8+ and CD4+ T cells, pDCs, and monocytes peripherally. Gene signatures associated with pR included increased pDC density, macrophage activation, and phagocytosis engulfment. Gut microbial signatures in pR were enriched for Gram-negative bacteria belonging to the Bacteroidaceae and Enterobacteriaceae families and to the small subgroup of Gram-negative Firmicutes. Our findings support that combined vidu/nivo treatment stimulates a broad antitumor immune response and is associated with a distinct baseline gut microbiome composition.
Heterogeneity and Co-occurrence in the Luminal and Mucosal Gut Microbiomes and Metabolomes of Oriental Rat snake (Ptyas mucosus)
Authors: Wei Yuting , Zhou Mingfang , Fang Wenjie , Liu Qiuhong , Mao Huirong , Chen Biao , Liu Sanfeng , Zhang Tianxiang , Xu Yongtao , Zhang Weiwei , Zheng Yunlin , Hu Xiaolong 
Published: November 2022

Abstract
Background
The gut microbiomes of animals have been widely investigated, but the effects of sampling sites in the gastrointestinal tract remain unclear. Previous studies regarding the gastrointestinal biogeography of microbiomes generally focused on longitudinal comparisons, whereas few studies have compared luminal and mucosal microbiomes. Investigations of the snake gut microbiome have attracted interest because of the unique digestive physiology and hibernation behavior, but adequate sampling methods must be developed. Here, we used an omics approach combining 16S rRNA gene sequencing with untargeted metabolomics to profile the luminal and mucosal gut microbiomes and metabolomes in oriental rat snakes, with the goal of revealing the heterogeneity and co-occurrence at these sites.
Results
The α-diversity of the gut microbiome was significantly higher at mucosal sites than at luminal sites. Microbial composition also differed according to sampling site, with significant differences in the abundances of dominant phyla and genera, as well as β-diversity clustering and distribution. Metabolome profiling revealed differences that were mainly related to cholinergic substances and nucleic acids. Analysis of variations in Kyoto Encyclopedia of Genes and Genomes functions of microbes and metabolites showed that the mucosal microbiome was more frequently involved in genetic information processing and cellular processes, whereas the luminal microbiome generally participated in metabolic regulation. Notably, we found a greater abundance of the opportunistic pathogen genus Escherichia-Shigella at luminal sites, and higher levels of the lipid-regulator metabolite fenfluramine at mucosal sites. Despite the extensive differences between the two sampling sites, the results revealed similarities in terms of amplicon sequence variant composition and dominant core microbes.
Conclusions
Our results partly support the hypothesis that the luminal microbiome is associated with metabolism, whereas the mucosal microbiome mainly contributes to immune function. Although studies of the snake microbiome have been limited, this pilot exploration of luminal and mucosal microbiomes and metabolites provides key insights to guide future research.
A Framework for Evaluating the Reusability of Software-As-A-Service Using Fuzzy Logic Approach
Authors: Deepika Deepika , Sangwan Om Prakash 
Published: November 2022

Abstract
Cloud computing provides software and hardware resources in the form of cloud services with the help of internet. Cloud Computing is also coming forward as an efficient criterion for reuse. Software-as-a Service is one of the three types of cloud services which provides on demand software services on pay-as-you-go basis. In order to make SaaS more advantageous and to assist end users efficiently, there is a need to build a reusability model for SaaS. Reusability plays a very vital role in developing a product easily and efficiently. In this paper, we have proposed a software reusability estimation model for SaaS. We have been used Fuzzy Logic Methodology for evaluating the SaaS reusability. For this model, we used the SaaS reusability attributes i.e., Commonality, Accessibility, Availability, Customizability and Efficiency. Centroid Method is used for validating the results obtained from the proposed approach. This model evaluates the reusability of SaaS services with the required accuracy level. On the basis of experiment results, it has been proved out that machine learning techniques gives enhanced accuracy of estimation as compared to other reusability estimation techniques, found helpful and more useful for SaaS service providers as well as users.
Exploring Intervention of Selenium on Gut Microbiota Homeostasis and Corresponding Genetic Metabolism in Mice with Breast Cancer on a High-fat Diet Using Metagenomics Sequencing
Authors: Li Yinan , Liu Min , Kong Bingtan , Zhang Ganlin , Zhang Qing 
Published: November 2022

Abstract
Background: The trace element selenium has an important effect on gut microbial homeostasis and the gut microbiota is closely related to breast cancer and its high-fat status. The effect of selenium on the gut microbiota of breast cancer in the high-fat state is unknown.
Materials and Methods: Twelve female BALB/c mice were randomly divided into two groups (4T1+selenium+fat diet group, 4T1+high fat diet group). To establish an obese mouse model, cultured 4T1 cells were transplanted on the right 4th mammary fat pad under anesthesia with 105 4T1 cells /50µl /mouse, and were given a high-fat diet for feeding. DNA was extracted from mouse fecal samples for meta-genomics sequencing and bioinformatics analysis. The relevant target genes and pathways were annotated and metabolically analyzed to explore the intervention effect of selenium on breast cancer in the high-fat state.
Results: Compared with the control group, the gut microbiota distribution and diversity were changed in breast cancer tumor bearing mice on a high-fat diet with selenium intervention. The gut microbial composition was significantly different in the selenium intervention group, with Proteobacteria, Actinobacteria, Verrucomicrobia phylum as well as the Helicobacter_ganmani，Helicobacter_japonicus and Akkermansia_muciniphila several species were increased and The phyla represented by Bacteroidetes, Firmicutes, Deferribacteres, Spirochaetes, as well as the Prevotella_sp_MGM2，Muribaculum_intestinale，Lactobacillus_murinus and Prevotella_sp_MGM1 several species of microbes were decreased. By functional analysis, a total of 21 significantly different functional genes associated with carbohydrate active enzymes were predicted, 455 significant genes of pathogen host interactions, 66 genes significantly associated with cell communication and cell auto-induction, 834 genes associated with membrane transporters, 220 genes related to virulence factors that were significantly different after selenium intervention. 37 cogs were predicted. 48 metabolites with rising metabolic potential in the selenium intervention group, such as L-alanine, SO2, and O2, among others.
Conclusions: Selenium can affect the homeostasis of gut microbiota by affecting the structure and abundance and associated metabolism of gut microbiota in mice with breast cancer on a high-fat diet. The mechanism may be through interfering with gut microbiota homeostasis, further affecting the synthesis of tumor associated proteins and fatty acids, and inducing tumor cell apoptosis and pyroptosis.
Development of mesothelioma-specific oncolytic vaccine exploiting immunopeptidomic analysis of murine and human tumors
Authors: Chiaro Jacopo , Antignani Gabriella , Feola Sara , Feodoroff Michaela , Martins Beatriz , Cojoc Hanne , Ferrari Valentina , Ciampi Daniele , Ilonen Ilkka , Räsänen Jari , Mäyränpää Mikko , Partanen Jukka , Koskela Satu , Kuryk Lukasz , Russo Salvatore , Fusciello Manlio , Hamdan Firas , Rescigno Maria , Grönholm Mikaela , Branca Rui Mamede , Lehtiö Janne , Cerullo Vincenzo 
Published: November 2022

Abstract
Malignant pleural mesothelioma (MPM) is an aggressive tumor with a poor prognosis. As the available therapeutic options show a lack of efficacy, novel treatments and therapeutic targets are urgently needed.
It has been observed that MPM is responsive to immunotherapeutic cancer treatments, and given its T-cell infiltration, we hypothesized that MPM is a suitable target for therapeutic cancer vaccination.
To date, research on mesothelioma has focused on the identification of molecular signatures to better classify and characterize the disease, and little is known about therapeutic targets to engage cytotoxic (CD8+) T cells. In this study, for the first time, we explored the immunopeptidomic antigen-presented landscape of MPM in both murine (AB12 cell line), human cell lines (H28, MSTO-211H, H2452, and JL1), and in patients' primary tumors. Applying state-of-art MHC immuno-affinity purification methodologies, we identified MHC-I-restricted peptides presented on the surface of malignant cells. We characterized some newly discovered patients-derived peptides utilizing in vitro co-culturing techniques, demonstrating that eluted peptides showed promising immunogenicity profiles. Additionally, we provided a proof-of-concept for the application of a whole antigen discovery pipeline using immunopeptidomics for cancer vaccine development in a murine model of MPM. Overall, we investigated the antigen landscape of MPM and showed that the discovered peptides show potential to be used for therapeutic cancer vaccine.