bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pre-cleaned bare wooden toothpicks for the determination of drugs in oral fluid by mass spectrometry
Authors: Millán-Santiago Jaime , Lucena Rafael , Cárdenas Soledad 
Published: March 2022

Abstract
This article deepens the potential of pre-cleaned bare wooden toothpicks (pb-WTs) for extracting drugs (antidepressants and acetaminophen) from oral fluid samples. The leaching of the intrinsic compounds from the wood matrix is identified as the main challenge for the final determination of the targets, even when a very selective instrumental technique, such as mass spectrometry, is employed. The pre-cleaning of the WTs is proposed for improving the analytical performance. The number of cleaning cycles depends on the injection mode (direct infusion or chromatography) into the mass spectrometer. The different variables affecting the extraction of selected antidepressant drugs were studied in detail, and the optimum procedure was validated using the two mentioned injection modes. The limits of detection were in the ranges 0.1–0.5 ng/mL and 0.1–0.3 ng/mL for direct infusion and liquid chromatography, respectively. The intra-day precision (expressed as relative standard deviation) was better than 12.1% and 8.6%, for direct infusion and liquid chromatography, respectively. Single-blind samples were used to study the applicability of the method. Finally, as a proof-of-concept, the potential of pb-WTs for in vivo sampling was outlined.
Graphical abstract
Diagnostic accuracy of automated 3D volumetry of cardiac chambers by CT pulmonary angiography for identification of pulmonary hypertension due to left heart disease
Authors: Melzig Claudius , Do Thuy Duong , Egenlauf Benjamin , Partovi Sasan , Grünig Ekkehard , Kauczor Hans-Ulrich , Heussel Claus Peter , Rengier Fabian 
Published: March 2022

Abstract
Objectives
To assess diagnostic accuracy of automated 3D volumetry of cardiac chambers based on computed tomography pulmonary angiography (CTPA) for the differentiation of pulmonary hypertension due to left heart disease (group 2 PH) from non-group 2 PH compared to manual diameter measurements.

Methods
Patients with confirmed PH undergoing right heart catheterisation and CTPA within 100 days for diagnostic workup of PH between August 2013 and February 2016 were included in this retrospective, single-centre study. Automated 3D segmentation of left atrium, left ventricle, right atrium and right ventricle (LA/LV/RA/RV) was performed by two independent and blinded radiologists using commercial software. For comparison, axial diameters were manually measured. The ability to differentiate group 2 PH from non-group 2 PH was assessed by means of logistic regression.

Results
Ninety-one patients (median 67.5 years, 44 women) were included, thereof 19 patients (20.9%) classified as group 2 PH. After adjustment for age, sex and mean pulmonary arterial pressure, group 2 PH was significantly associated with larger LA volume (p < 0.001), larger LV volume (p = 0.001), lower RV/LV volume ratio (p = 0.04) and lower RV/LA volume ratio (p = 0.003). LA volume demonstrated the highest discriminatory ability to identify group 2 PH (AUC, 0.908; 95% confidence interval, 0.835–0.981) and was significantly superior to LA diameter (p = 0.009). Intraobserver and interobserver agreements were excellent for all volume measurements (intraclass correlation coefficients 0.926–0.999, all p < 0.001).

Conclusions
LA volume quantified by automated, CTPA-based 3D volumetry can differentiate group 2 PH from other PH groups with good diagnostic accuracy and yields significantly higher diagnostic accuracy than left atrial diameter.

Key Points
• Automated cardiac chamber volumetry using non-gated CT pulmonary angiography can differentiate pulmonary hypertension due to left heart disease from other causes with good diagnostic accuracy.
• Left atrial volume yields significantly higher diagnostic accuracy than left atrial axial diameter for identification of pulmonary hypertension due to left heart disease without time-consuming manual processing.
Impact of energy drink versus coffee consumption on periodic repolarization dynamics: an interventional study
Authors: Schüttler Dominik , Rudi Wolf-Stephan , Bauer Axel , Hamm Wolfgang , Brunner Stefan 
Published: March 2022

Abstract
Purpose
Caffeinated beverages are consumed daily throughout the world. Caffeine consumption has been linked to dysfunction of the autonomic nervous system. However, the exact effects are still insufficiently understood.

Methods
Sixteen healthy individuals were included in the present non-randomized cross-over interventional study. All study subjects consumed a commercial energy drink (containing 240 mg caffeine), and in a second independent session coffee (containing 240 mg caffeine). High-resolution digital ECGs in Frank-lead configuration were recorded at baseline before consumption, and 45 min after consumption of the respective beverage. Using customized software, we assessed ECG-based biomarker periodic repolarization dynamics (PRD), which mirrors the effect of efferent cardiac sympathetic activity on the ventricular myocardium.

Results
The consumption of energy drinks resulted in an increase in PRD levels (3.64 vs. 5.85 deg2; p < 0.001). In contrast, coffee consumption did not alter PRD levels (3.47 vs 3.16 deg2, p = 0.63). The heart rates remained unchanged both after coffee and after energy drink consumption. Spearman analysis showed no significant correlation between PRD changes and heart rate changes (R = 0.34, p = 0.31 for coffee, R = 0.31, p = 0.24 for energy drink).

Conclusion
Our data suggests that sympathetic activation after consumption of caffeinated beverages is independent from caffeine and might be mediated by other substances.
Trial Number: NCT04886869, 13 May 2021, retrospectively registered
Fluorescence lifetime imaging and electron microscopy: a correlative approach
Authors: Wieland Johannes G. , Naskar Nilanjon , Rück Angelika , Walther Paul 
Published: March 2022

AbstractFluorescence lifetime imaging microscopy (FLIM) allows the characterization of cellular metabolism by quantifying the rate of free and unbound nicotinamide adenine dinucleotide hydrogen (NADH). This study delineates the correlative imaging of cells with FLIM and electron microscopy (EM). Human fibroblasts were cultivated in a microscopy slide bearing a coordinate system and FLIM measurement was conducted. Following chemical fixation, embedding in Epon and cutting with an ultramicrotome, tomograms of selected cells were acquired with a scanning transmission electron microscope (STEM). Correlative imaging of antimycin A-treated fibroblasts shows a decrease in fluorescence lifetime as well as swollen mitochondria with large cavities in STEM tomography. To our knowledge, this is the first correlative FLIM and EM workflow. Combining the high sensitivity of FLIM with the high spatial resolution of EM could boost the research of pathophysiological processes involving cell metabolism, such as cancer, neurodegenerative disorders, and viral infection.
Impact of rapid maxillary expansion on palatal morphology at different dentition stages
Authors: Kinzinger Gero Stefan Michael , Lisson Jörg Alexander , Buschhoff Charlotte , Hourfar Jan , Korbmacher-Steiner Heike 
Published: March 2022

Abstract
Objective
Rapid maxillary expansion (RME) is an established and frequently used procedure to overcome maxillary constriction. In-depth studies about morphological changes of the alveolar process and its immediate surroundings are missing. Therefore, the aim of the present study was to examine the treatment effects of a dentally anchored, rapid maxillary expander at different dentition stages upon palatal width, height and shape.

Material and methods
The dental casts of 114 patients—taken immediately before and after RME—were three-dimensionally analysed. Depending on the dentition stage, the patients were divided into two groups (each n = 57, group 1, early mixed dentition; group 2, late mixed or permanent dentition).

Results
The width increases were highly significant, both in the overall and in the individual groups (p < 0.001). While the width increase was greater in the posterior area than anteriorly in the early group, the widening in the late group happened significantly greater anteriorly than posteriorly. Palatal height increased anteriorly and posteriorly in both groups to a significant extent (p < 0.001). The height increase was more pronounced in the anterior region than in the posterior region in the late group. The palatine index according to Kim revealed a change in palatal morphology both anteriorly and posteriorly in the early group but only anteriorly in the late group.

Conclusions
Maxillary expansion occurs more parallel in early treatment compared to V-shaped opening in the later treatment approach.

Clinical relevance
RME is more advantageous in an early dentition.
Place-based power production deliberations in Saskatchewan: engaging future sustainability
Authors: Hurlbert Margot A. 
Published: March 2022

Abstract
This article addresses a gap in knowledge of peoples’ strategies and recommendations concerning power production and achieving reductions in GHGs to address climate change. Employing mixed methods, two-day deliberative focus groups in three communities in Saskatchewan, Canada included pre and post-focus group surveys, coding and analysis of discussions, and the creation of consensus recommendations for sustainable power production in the future. These innovative mixed-methods provide insights into how to advance individual and social learning. Results of comparative case study analysis provide strong support for renewables and illustrate place-based differences. All communities supported renewable sources. The community in proximity to coal, oil, and gas production supported coal, and coal with carbon capture and storage (CCS) and was concerned with the social cost of job loss on the welfare system; engaging the public was not a priority. In contrast, the other two communities stressed the importance of engaging the public and considering all costs, risks, benefits across the entire lifespan of power production sources. To achieve future sustainability, policy implications include addressing important concerns of resource-dependent communities, namely job loss, and conducting holistic policy assessment of potential power production sources that account for carbon and cost across the entire supply chain and include land-use change.

Graphical abstract
Seismic vulnerability of historic brick masonry buildings in Vienna
Authors: Karic Amel , Atalić Josip , Kolbitsch Andreas 
Published: March 2022

AbstractThis paper presents a seismic vulnerability analysis of historic brick masonry buildings in Vienna, Austria from the period of the Austro-Hungarian monarchy (1840–1918). The vulnerability study is based on comprehensive data from the devastating earthquake damage to the masonry buildings in Zagreb, Croatia on March 22, 2020, which are from the Austro-Hungarian monarchy and comparable to Viennese buildings from that period, as well as on comprehensive numerical structural analyses calibrated on in situ test series. The statistical analysis of the earthquake damage to the Zagreb masonry stock and the comprehensive numerical simulations, allowed profound conclusions about the proportional damage distribution over individual structural areas of the masonry buildings, considering construction-specific characteristics such as the building height, the structural regularity/irregularity or the construction type under the ground level. This study enhances the still limited knowledge about the vulnerability of the historic brick masonry buildings from the period of the Austro-Hungarian monarchy and allows extensive conclusions about the seismic vulnerability of these buildings.
Occupancy and co-occurrence patterns of endemic mammals and introduced predators across a broad geographical gradient in eastern Australia
Authors: McHugh Darren , Goldingay Ross L. , Letnic Mike 
Published: March 2022

AbstractInvasive predators, land clearing and altered fire regimes have been implicated in species decline and extinction worldwide. Enhanced knowledge of how these factors interact and influence medium-sized mammals is warranted. We tested three hypotheses using occupancy data for a diverse mammal assemblage including three threatened species, five common species, two introduced mesopredators and an apex predator in eastern Australia. We hypothesised that occupancy of mammal species within the assemblage would be influenced by (i) the physical environment (rainfall, vegetation type and elevation), (ii) habitat disturbance (number of fires and habitat fragmentation) and (iii) mesopredator release, whereby occupancy and/or detection of medium-sized mammals are influenced by mesopredators, the feral cat (Felis catus) and the red fox (Vulpes vulpes), which are influenced by an apex predator, the dingo (Canis familiaris). We utilised camera-trapping data from 173 sites (692 camera locations) across a north–south gradient spanning ~ 1500 km in eastern Australia. Although hypotheses i (physical environment) and ii (habitat disturbance) are not mutually exclusive, we show that the variables considered in each were only weakly correlated. We conducted occupancy modelling to investigate the physical environment and habitat disturbance hypotheses. We conducted co-occurrence modelling to investigate interactions between species. The physical environment hypothesis best supported occupancy models for six mammal species: red-necked pademelon (Thylogale thetis), bandicoots (Isoodon macrourus and Perameles nasuta), swamp wallaby (Wallabia bicolor), red-necked wallaby (Macropus rufogriseus), eastern grey kangaroo (Macropus giganteus) and feral cat. The disturbance hypothesis best supported occupancy models for four mammal species: long-nosed potoroo (Potorous tridactylus), red-necked pademelon and both mesopredators. Support for the mesopredator release hypothesis was equivocal. Large macropods showed site avoidance towards the red fox. Four species showed higher detection at sites where mesopredators were not detected. The fox showed a negative detection interaction to the dingo and the cat did not. Our study highlights how factors such as rainfall, land clearing, elevation and number of fires influence the occupancy of species within a diverse mammal assemblage at the macroecological scale. Our findings have implications for the conservation of threatened species in managed landscapes and suggestions for further research following the recent 2019–2020 wildfires.
Biocultural diversity in the traditional landscape of Vallecorsa
Authors: De Pasquale Giorgia , Livia Sagliocco 
Published: March 2022

AbstractIn Italy, agricultural intensification and the abandonment of rural areas since the 1960s has led to a transformation of the traditional agricultural landscape, bringing with it a series of environmental and socio-economic issues and problems such as those linked to hydrogeological instability, a reduction in biocultural diversity and migration to urban centres, especially of the younger population. These phenomena have also led to a progressive loss of all the cultural and traditional practices related to these systems, thus contributing to a homogenization of the landscape structure due to abandonment. On the other hand, in the last years, we are witnessing a return to rural and traditional practices with the aim of recovering local cultures, especially those associated to agricultural practices, and to a more sustainable management of the territory. Vallecorsa, a municipality in central Italy, is one of the landscapes inscribed in the Italian National Register of Historical Rural Landscapes and it is still characterized by traditional agricultural practices that provide multiple benefits also in ecological terms, maintaining a specific level of biodiversity, interpreted as part of the biocultural diversity of the area. The aim of the present research is to analyse the structure of the traditional landscape of Vallecorsa and to determine its main characteristics, using landscape as the scale. Through the use of GIS software, by means of photo-interpretation and the use of DTM, the present study analyses the structure of land use, and in particular of terraced surfaces, and the associated data on area size, fragmentation, altitude and slope. The results take into account the diversity of land uses and mosaic patches, as well as terraced surfaces, and their distribution in the territory, relating them to the local biodiversity, in particular that of dry stone walls.
Structural Decomposition Analysis of Japan’s Energy Transitions and Related CO2 Emissions in 2005–2015 Using a Hybrid Input-Output Table
Authors: Ueda Tatsuki 
Published: March 2022

AbstractThis study investigates Japan’s energy transitions in 2005–2015, which involved massive economic disruptions due to the 2011 Great East Japan earthquake and the Great Recession. A hybrid input-output (IO) table that conforms to the energy conservation condition was newly compiled by integrating the Japanese energy-balance and linked-IO tables. This was employed to conduct a structural decomposition analysis (SDA), which attributes changes in energy consumption and CO2 emissions to the effects of intensity, structure, domestic final demand, and export. These effects were successfully segregated into a profile of energy sources. The results revealed that the structural effect became the dominant driver for decisively reducing energy consumption and emissions of manufacturing and service sectors in the latter period 2011–2015. This suggested that it took time to materialize energy-saving innovations in response to the sudden economic disruptions. Over the entire period, the structural effect was the largest driver contributing to the overall reductions, in part because the other effects tended to cancel out either between energy sources or periods. Therefore, a sensible way to transform Japan to a less energy-intensive, carbon-free society in the future is to improve the non-energy input structures of the manufacturing and service sectors.
An integrated approach to explore the suitability of nitrate-contaminated groundwater for drinking purposes in a semiarid region of India
Authors: Panneerselvam Balamurugan , Muniraj Kirubakaran , Duraisamy Karunanidhi , Pande Chaitanya , Karuppannan Shankar , Thomas Maciej 
Published: March 2022

AbstractThe main objective of the present study is to perform risk assessment of groundwater contaminated by nitrate (NO3−) and evaluate the suitability of groundwater for domestic purposes in the Palani region of South India. Thirty groundwater samples were collected in the study area. Various groundwater quality analysis parameters such as the pH, electrical conductivity, total dissolved solids, total hardness, major cations (Ca2+, Mg2+, Na+, and K+), and major anions (Cl−, SO42−, F−, CO32−, and HCO3−) were adopted in this study to evaluate the drinking water suitability according to 2011 World Health Organization (WHO) standards. Piper and Gibbs’s diagrams for the tested groundwater indicated that, due to the influence of rock–water interactions, evaporation, and reverse ion exchange, the chemical composition of groundwater varied. According to water quality index (WQI) mapping results, 46.67% of the sample locations was identified as contaminated zones via GIS spatial analysis. Multivariate statistical analysis methods, such as principal component analysis, cluster analysis, and the Pearson correlation matrix, were applied to better understand the relationship between water quality parameters. The results demonstrated that 40% of the samples could be identified as highly affected zones in the study region due to a high nitrate concentration. The noncarcinogenic health risks among men, women, and children reached 40, 50, and 53%, respectively. The results illustrated that children and women occurred at a higher risk than did men in the study region. The major sources of contamination included discharge from households, uncovered septic tanks, leachate from waste dump sites, and excess utilization of fertilizers in the agricultural sector. Furthermore, using the nitrate health hazard integrated method with the conventional indexing approach ensures that groundwater reliability can be guaranteed, contamination can be explored, and appropriate remedial measures can be implemented.
Vibrational properties of the mononuclear Fe[HBpz3]2 spin crossover complex
Authors: Hochdörffer Tim , Wolny Juliusz A. , Omlor Andreas , Scherthan Lena , Auerbach Hendrik , Müller Christina S. , Wille Hans-Christian , Sergeev Ilya , Schünemann Volker 
Published: March 2022

AbstractWithin this work, we report the results of nuclear inelastic scattering experiments of the low-spin phase of the iron(II) mononuclear SCO complex Fe[HBpz3]2 and density functional theory based calculations performed on a model molecule of the complex. We show that the calculated partial density of vibrational states based on the structure of a single iron(II) center which is linked by three pyrazole rings to borat is in good accordance with the experimentally obtained 57Fe-pDOS and assign the molecular vibrations to the prominent optical phonons.
Future Industry Internet of Things with Zero-trust Security
Authors: Li Shan , Iqbal Muddesar , Saxena Neetesh 
Published: March 2022

AbstractThe emerging techniques, such as the fifth-generation communications (5G), Internet of Things (IoT), blockchain, artificial intelligence, etc., are operating in unison will drive the transformation of global business forward. The 5G technology is expected to unleash a massive IoT ecosystems by providing massive connectivity for huge number of IoT devices with faster data rate, ultra-low latency, and low-cost access. The 5G networks will be designed to bring the level of performance needed for massive IoT and will enable a perceived fully ubiquitous connected world. Meanwhile, the blockchain being promoted as the fundamental for new business model in Future IoT (FIoT). This paper attempts to provide a set of new directions and ideas for research in 5G/6G enabled IoT and new technique trends in IoT. The current IoT are facing a number of challenges, such as massive IoT devices access, network performances, security, standardization, and critical applications. This paper investigates new technologies, such as 5G, zero-trust, and blockchain will catalyse the innovation in IoT. Specifically, a zero-trust security architecture for FIoT is proposed and a blockchain-based device authentication in IoT environment (BasIoT) is proposed that can provide massive secure device access in FIoT.