bluuebunny/crossref_metadata_2025_split huggingface dataset:
PEMETAAN EFEK SPASIAL KEMISKINAN SELURUH KABUPATEN DI INDONESIA
Authors: Hasibuan Sarah , Hasibuan Mutia Harum 
Published: January 2022

Kemiskinan menjadi salah satu penghambat sulitnya suatu daerah/negara untuk maju. Kemiskinan menyebabkan menurunnya kualitas sumberdaya manusia, akibat ketidakmampuan mengakses pendidikan, kesehatan, nutrisi yang baik yang berimbas pada produktivitas yang melemah. Studi ini bertujuan untuk menganalisis pola sebaran kemiskinan seluruh kabupaten di Indonesia dengan memakai metode Moran’s Index. Selain itu studi ini juga melihat sebaran spasial kemiskinan secara lokal di setiap kabupaten dengan pendekatan Local Indicator of Spatial Autocorrelation (LISA). Adapun periode data yang digunakan adalah tahun 2019. Hasil studi mengemukakan kantong-kantong kemiskinan di Indonesia sebagian besar terletak di Provinsi Papua, Papua Barat dan Nusa Tenggara Timur. Kabupaten dengan kemiskinan terendah terdapat di kabupaten Provinsi Bali dan Kalimantan Selatan. Hasil perhitungan Uji Moran terdapat autokorelasi positif sebesar 0,474259 yang menunjukkan adanya keterkaitan spasial secara umum di seluruh kabupaten di Indonesia dengan pola yang menggerombol (clustred). Sementara hasil uji LISA memetakan tingkat kemiskinan penduduk dalam empat kelompok, yakni: terdapat 50 Kabupaten yang berada pada kriteria kabupaten dengan kemiskinan tinggi dikelilingi kabupaten kemiskinan tinggi (high-high), 69 kabupaten yang masuk dalam kriteria kemiskinan rendah dikelilingi kabupaten kemiskinan rendah (low-low), 12 kabupaten pada kriteria kemiskinan rendah, dikelilingi kabupaten kemiskinan tinggi (low-high) dan 5 kabupaten kemiskinan tinggi dikelilingi kabupaten kemiskinan rendah (high-low). Perbedaan tingkat kemiskinan menunjukkan penanganan/penuntasan kemiskinan tidak dapat diseragamkan setiap daerah
240 ЛЕТ НА РОССИЙСКОЙ ЗЕМЛЕ (ИЗ ИСТОРИИ ОСНОВАННОГО АРМЯНАМИ ГОРОДА НОВОГО НАХИЧЕВАНА)
Authors: Тер-Саркисянц А.Е. 
Published: January 2022

Статья посвящена истории города Нового Нахичевана, основанного в 1779 г. переселенными по указу Екатерины II из Крыма в Россию армянами, получившими при этом от правительства ряд льгот и привилегий. Новый город успешно развивался и благоустраивался за счет купеческой торговли, развития ремесел, промышленности, благотворной деятельности меценатов, способствовавших строительству в нем учебных заведений, церквей, театра, музея и других культурных объектов, в результате чего он вскоре стал одним из экономически и культурно важных центров на Юге России. Но с присоединением Нового Нахичевана в декабре 1928 г. к соседнему городу Ростову-на-Дону, в котором он первое время существовал в виде Нахичеванского района, а затем с переименованием в 1929 г. в ни о чем не говорящий Пролетарский район, историческое название города, в котором прочно сохранялся национальный быт его жителей и уроженцами которого были многие выдающиеся армяне, исчезло, а в дальнейшем, особенно в 1920–1930-е гг. сталинских репрессий, существенно пострадали и сам город, и его известные горожане. В наше время, на фоне возрождающегося интереса к своей истории, донскими армянами все чаше стали высказываться пожелания о возвращении городу его прежнего названия, что стало бы актом большого нравственного значения.
Manipulating CD4+ T Cell Pathways to Prevent Preeclampsia
Authors: Murray Eileen J. , Gumusoglu Serena B. , Santillan Donna A. , Santillan Mark K. 
Published: January 2022

Preeclampsia (PreE) is a placental disorder characterized by hypertension (HTN), proteinuria, and oxidative stress. Individuals with PreE and their children are at an increased risk of serious short- and long-term complications, such as cardiovascular disease, end-organ failure, HTN, neurodevelopmental disorders, and more. Currently, delivery is the only cure for PreE, which remains a leading cause of morbidity and mortality among pregnant individuals and neonates. There is evidence that an imbalance favoring a pro-inflammatory CD4+ T cell milieu is associated with the inadequate spiral artery remodeling and subsequent oxidative stress that prime PreE’s clinical symptoms. Immunomodulatory therapies targeting CD4+ T cell mechanisms have been investigated for other immune-mediated inflammatory diseases, and the application of these prevention tactics to PreE is promising, as we review here. These immunomodulatory therapies may, among other things, decrease tumor necrosis factor alpha (TNF-α), cytolytic natural killer cells, reduce pro-inflammatory cytokine production [e.g. interleukin (IL)-17 and IL-6], stimulate regulatory T cells (Tregs), inhibit type 1 and 17 T helper cells, prevent inappropriate dendritic cell maturation, and induce anti-inflammatory cytokine action [e.g. IL-10, Interferon gamma (IFN-γ)]. We review therapies including neutralizing monoclonal antibodies against TNF-α, IL-17, IL-6, and CD28; statins; 17-hydroxyprogesterone caproate, a synthetic hormone; adoptive exogenous Treg therapy; and endothelin-1 pathway inhibitors. Rebalancing the maternal inflammatory milieu may allow for proper spiral artery invasion, placentation, and maternal tolerance of foreign fetal/paternal antigens, thereby combatting early PreE pathogenesis.
Spatially Resolved Operando Synchrotron-Based X-Ray Diffraction Measurements of Ni-Rich Cathodes for Li-Ion Batteries
Authors: Leach Andrew Stephen , Llewellyn Alice V. , Xu Chao , Tan Chun , Heenan Thomas M. M. , Dimitrijevic Alex , Kleiner Karin , Grey Clare P. , Brett Dan J. L. , Tang Chiu C. , Shearing Paul R. , Jervis Rhodri 
Published: January 2022

Understanding the performance of commercially relevant cathode materials for lithium-ion (Li-ion) batteries is vital to realize the potential of high-capacity materials for automotive applications. Of particular interest is the spatial variation of crystallographic behavior across (what can be) highly inhomogeneous electrodes. In this work, a high-resolution X-ray diffraction technique was used to obtain operando transmission measurements of Li-ion pouch cells to measure the spatial variances in the cell during electrochemical cycling. Through spatially resolved investigations of the crystallographic structures, the distribution of states of charge has been elucidated. A larger portion of the charging is accounted for by the central parts, with the edges and corners delithiating to a lesser extent for a given average electrode voltage. The cells were cycled to different upper cutoff voltages (4.2 and 4.3 V vs. graphite) and C-rates (0.5, 1, and 3C) to study the effect on the structure of the NMC811 cathode. By combining this rapid data collection method with a detailed Rietveld refinement of degraded NMC811, the spatial dependence of the degradation caused by long-term cycling (900 cycles) has also been shown. The variance shown in the pristine measurements is exaggerated in the aged cells with the edges and corners offering an even lower percentage of the charge. Measurements collected at the very edge of the cell have also highlighted the importance of electrode alignment, with a misalignment of less than 0.5 mm leading to significantly reduced electrochemical activity in that area.
Nitric Oxide in Selective Cerebral Perfusion Could Enhance Neuroprotection During Aortic Arch Surgery
Authors: Linardi Daniele , Mani Romel , Murari Angela , Dolci Sissi , Mannino Loris , Decimo Ilaria , Tessari Maddalena , Martinazzi Sara , Gottin Leonardo , Luciani Giovanni B. , Faggian Giuseppe , Rungatscher Alessio 
Published: January 2022

BackgroundHypothermic circulatory arrest (HCA) in aortic arch surgery has a significant risk of neurological injury despite the newest protective techniques and strategies. Nitric oxide (NO) could exert a protective role, reduce infarct area and increase cerebral perfusion. This study aims to investigate the possible neuroprotective effects of NO administered in the oxygenator of selective antegrade cerebral perfusion (SCP) during HCA.MethodsThirty male SD adult rats (450–550 g) underwent cardiopulmonary bypass (CPB), cooling to 22°C body core temperature followed by 30 min of HCA. Rats were randomized to receive SCP or SCP added with NO (20 ppm) administered through the oxygenator (SCP-NO). All animals underwent CPB-assisted rewarming to a target temperature of 35°C in 60 min. At the end of the experiment, rats were sacrificed, and brain collected. Immunofluorescence analysis was performed in blind conditions.ResultsNeuroinflammation assessed by allograft inflammatory factor 1 or ionized calcium-binding adapter molecule 1 expression, a microglia activation marker was lower in SCP-NO compared to SCP (4.11 ± 0.59 vs. 6.02 ± 0.18%; p &lt; 0.05). Oxidative stress measured by 8oxodG, was reduced in SCP-NO (0.37 ± 0.01 vs. 1.03 ± 0.16%; p &lt; 0.05). Brain hypoxic area extent, analyzed by thiols oxidation was attenuated in SCP-NO (1.85 ± 0.10 vs. 2.74 ± 0.19%; p &lt; 0.05). Furthermore, the apoptotic marker caspases 3 was significantly reduced in SCP-NO (10.64 ± 0.37 vs. 12.61 ± 0.88%; p &lt; 0.05).ConclusionsNitric oxide administration in the oxygenator during SCP and HCA improves neuroprotection by decreasing neuroinflammation, optimizing oxygen delivery by reducing oxidative stress and hypoxic areas, finally decreasing apoptosis.
Radiofrequency Ablation of Parathyroid Glands to Treat a Patient With Hypercalcemia Caused by a Novel Inactivating Mutation in CaSR
Authors: Hao Yu , Lei Zhikai , Shi Nanjing , Yu Lingying , Ji Weiqin , Zhang Xianfeng 
Published: January 2022

ObjectiveWe identified a novel inactivating mutation in the calcium-sensing receptor (CaSR) gene in a patient with refractory hypocalciuric hypercalcemia and analyzed its function. The effectiveness of radiofrequency ablation of the parathyroid glands to treat hypercalcemia caused by this mutation was explored.MethodsClinical data of patients before and after radiofrequency ablation were retrospectively analyzed. The CaSR mutation (D99N) found in the patient was studied in cell lines. HEK-293 cells were transfected with plasmids containing wild-type (WT) or mutant CaSR genes (D99N and W718X). Expression levels of the respective CaSR proteins were measured, and their functions were assessed by examining the effect of NPS R-568 (a CaSR agonist) on intracellular Ca2+ oscillations and that of exogenous parathyroid hormone (PTH) on intracellular cyclic adenosine monophosphate (cAMP) levels.ResultsThe effectiveness of pharmacological treatment was poor, whereas radiofrequency ablation of the parathyroid glands resulted in controlled blood calcium and PTH levels in the patient. In cell lines, upon NPS R-568 administration, the amplitude of intracellular Ca2+ oscillations in the D99N group was lower than that in the WT group and higher than that in the W718X group. Upon administration of PTH, intracellular cAMP levels in the D99N group were higher than those in the WT group and lower than those in the W718X group.ConclusionThe homozygous mutation D99N reduced CaSR activity and caused more severe hypocalciuric hypercalcemia. For patients with this type of hypercalcemia and poor response to pharmacological treatments, radiofrequency ablation of the parathyroid glands may be a suitable treatment option.
Free and Immobilized Microbial Culture–Mediated Crude Oil Degradation and Microbial Diversity Changes Through Taxonomic and Functional Markers in a Sandy Loam Soil
Authors: Khandelwal Ashish , Sugavanam Ramya , Ramakrishnan B. , Dutta Anirban , Varghese Eldho , Nain Lata , Banerjee Tirthankar , Singh Neera 
Published: January 2022

Crude oil contamination of soil and water resources is a widespread issue. The present study evaluated the degradation of aliphatic hydrocarbons (C11–C36) in crude oil by 17 bacteria isolated from a crude oil–contaminated soil. The results suggested that Pseudomonas sp. and Bacillus amyloliquefaciens were the best hydrocarbon-degrading bacteria in the presence of surfactant Tween-80 (0.1% w/v). Based on the present investigation and a previous study, Pseudomonas sp. + B. amyloliquefaciens and fungus Aspergillus sydowii were identified as best oil degraders and were immobilized in alginate–bentonite beads, guargum–nanobenonite water dispersible granules (WDGs), and carboxy methyl cellulose (CMC)–bentonite composite. Sandy loam soil was fortified with 1, 2, and 5% crude oil, and total petroleum hydrocarbon (TPH) degradation efficiency of free cultures and bio-formulations was evaluated in sandy loam soils. Compared to a half-life (t1/2) of 69.7 days in the control soil (1% oil), free cultures of Pseudomonas sp. + B. amyloliquefaciens and A. sydowii degraded TPH with t1/2 of 10.8 and 19.4 days, respectively. Increasing the oil content slowed down degradation, and the t1/2 in the control and soils inoculated with Pseudomonas sp. + B. amyloliquefaciens and A. sydowii was 72.9, 14.7, and 22.2 days (2%) and 87.0, 23.4, and 30.8 days (5%), respectively. Supplementing soil with ammonium sulfate (1%) enhanced TPH degradation by Pseudomonas sp. + B. amyloliquefaciens (t1/2–10 days) and A. sydowii (t1/2–12.7 days). All three bio-formulations were effective in degrading TPH (1%), and the t1/2 was 10.7–11.9 days (Pseudomonas sp. + B. amyloliquefaciens and 14–20.2 days (A. sydowii) and were at par with free cultures. Microbial diversity analysis based on taxonomic markers and functional markers suggested that the bioaugmentation process helped keep soil in the active stage and restored the original microbial population to some extent. The present study concluded that bio-formulations of crude oil–degrading microbes can be exploited for its degradation in the contaminated environment.
Clinical Utility of the Prenatal BACs-on-Beads™ Assay in Invasive Prenatal Diagnosis
Authors: Jiang Yu , Wu Lili , Ge Yunshen , Zhang Jian , Huang Yanru , Wu Qichang , Zhang Yanhong , Zhou Yulin 
Published: January 2022

Background: The prenatal BACs-on-Beads™ (PNBoBs™) assay has been applied worldwide for prenatal diagnosis. However, there are neither guidelines nor consensus on choosing patients, sample types, or clinical pathways for using this technique. Moreover, different perspectives have emerged regarding its clinical value. This study aimed to evaluate its clinical utility in the context of clinical practice located in a prenatal diagnostic center in Xiamen, a city in southeast China.Methods: We tested 2,368 prenatal samples with multiple referral indications using both conventional karyotyping and PNBoBs™. Positive results from PNBoBs™ were verified using current gold-standard approaches.Results: The overall rates for the detection of pathogenic copy number variation (pCNV) by karyotyping and PNBoBs™ were 1.9% (46/2,368) and 2.0% (48/2,368), respectively. The overall detection rate of karyotyping combined with PNBoBs™ for pCNV was 2.3% (54/2,368). A total of 13 cases of copy number variation (CNV)with a normal karyotype were detected by PNBoBs™. Another case with a normal karyotype that was detected as a CNV of sex chromosomes by PNBoBs™ was validated to be maternal cell contamination by short tandem repeat analysis.Conclusion: Karyotyping combined with PNBoBs™ can improve both the yield and efficiency of prenatal diagnosis and is appropriate in the second trimester in all patients without fetal ultrasound anomalies who undergo invasive prenatal diagnosis.
STING Signaling Drives Production of Innate Cytokines, Generation of CD8+ T Cells and Enhanced Protection Against Trypanosoma cruzi Infection
Authors: Vieira Raquel de Souza , Nascimento Marilda Savoia , Noronha Isaú Henrique , Vasconcelos José Ronnie Carvalho , Benvenuti Luiz Alberto , Barber Glen N. , Câmara Niels Olsen Saraiva , Kalil Jorge , Cunha-Neto Edecio , Almeida Rafael Ribeiro 
Published: January 2022

A variety of signaling pathways are involved in the induction of innate cytokines and CD8+T cells, which are major players in protection against acuteTrypanosoma cruziinfection. Previous data have demonstrated that a TBK-1/IRF3-dependent signaling pathway promotes IFN-β production in response toTrypanosoma cruzi, but the role for STING, a main interactor of these proteins, remained to be addressed. Here, we demonstrated that STING signaling is required for production of IFN-β, IL-6, and IL-12 in response toTrypanosoma cruziinfection and that STING absence negatively impacts activation of IRF-dependent pathways in response to the parasite. We reported no significant activation of IRF-dependent pathways and cytokine expression in RAW264.7 macrophages in response to heat-killed trypomastigotes. In addition, we showed that STING is essential forT. cruziDNA-mediated induction of IFN-β, IL-6, and IL-12 gene expression in RAW264.7 macrophages. We demonstrated that STING-knockout mice have significantly higher parasitemia from days 5 to 8 of infection and higher heart parasitism at day 13 after infection. Although we observed similar heart inflammatory infiltrates at day 13 after infection, IFN-β, IL-12, CXCL9, IFN-γ, and perforin gene expression were lower in the absence of STING. We also showed an inverse correlation between parasite DNA and the expression of CXCL9, IFN-γ, and perforin genes in the hearts of infected animals at day 13 after infection. Finally, we reported that STING signaling is required for splenic IFN-β and IL-6 expression early after infection and that STING deficiency results in lower numbers of splenic parasite-specific IFN-γ and IFN-γ/perforin-producing CD8+T cells, indicating a pivotal role for STING signaling in immunity toTrypanosoma cruzi.
Therapeutic Management of Ocular Ischemia in Takayasu’s Arteritis: A Case-Based Systematic Review
Authors: Zeng Yue , Duan Jianan , Ge Ge , Zhang Meixia 
Published: January 2022

BackgroundTakayasu’s arteritis (TA) is a rare, chronic granulomatous large-vessel vasculitis that can lead to ocular ischemia. Ocular outcomes after therapeutic management in TA remain largely unknown. We herein conduct a case-based systematic review to address the current treatment options in this particular cohort.MethodsPubMed, Medline, and EMBASE databases were searched pertaining to ocular outcomes after systemic treatment in TA. Studies reporting ocular examinations before and after treatment in TA patients with ocular ischemia were included. Clinical characteristics, therapies, ocular outcomes, and complications were recorded.ResultsA 29-year-old woman with newly diagnosed TA showed dramatic regression of Takayasu’s retinopathy (TR) following balloon angioplasty. Optical coherence tomography angiography (OCTA) was used as a novel strategy for subsequent follow-up. A total of 117 eyes of 66 patients with a median age of 27 years were included for systematic review. TR was the most common ocular manifestation. Oral steroids were prescribed in nearly all patients (n = 65), followed by the use of methotrexate and antiplatelet therapy. Of the patients, 65.8% and 34.2% underwent open surgery and endovascular procedure, respectively. The median follow-up period was 12 weeks (interquartile range 8–33.5). Surgical therapy showed better ocular improvement (including visual and imaging responses) in both acute and chronic vision loss, along with fewer complications than medical therapy alone. In the surgical group, the visual prognosis was significantly better in patients with initial visual acuity better than 20/200 (p = 0.03) and those who underwent surgery before stage III TR (p = 0.01). Ocular outcomes were equivalent in the two surgical approaches.ConclusionClinicians should be familiar with ophthalmic manifestations of this potentially treatable complication in TA. Compared with medical therapy alone, surgical intervention might be a better choice for both acute and chronic vision loss. Surgery is best recommended before the onset of irreversible ischemia to the globe. A combined regimen (oral steroids, immunosuppressants, and antiplatelet drugs) might be effective for those with surgical contradictions or reluctance to an invasive procedure. Physicians should be aware of the importance of ocular examinations, including OCTA, during the diagnosis and follow-up in TA.
The Immunological Role of CDK4/6 and Potential Mechanism Exploration in Ovarian Cancer
Authors: Liu Chen , Huang Yuhan , Cui Yaoyuan , Zhou Jun , Qin Xu , Zhang Li , Li Xi , Li Yuan , Guo Ensong , Yang Bin , Li Xi , Fan Junpeng , Li Xiong , Fu Yu , Liu Si , Hu Dianxing , Xiao Rourou , Wang Zizhuo , Dou Yingyu , Wang Wei , Li Wenting , Yang Xiaohang , Liu Jingbo , Peng Wenju , Qin Tianyu , You Lixin , Lu Funian , Sun Chaoyang 
Published: January 2022

BackgroundOvarian cancer (OC) is one of the most lethal gynecologic cancers. Growing evidence has proven that CDK4/6 plays a key role in tumor immunity and the prognosis of many cancers. However, the expression and function of CDK4/6 in OC remain unclear. Therefore, we aimed to explore the influence of CDK4/6 in OC, especially on immunity.MethodsWe analyzed CDK4/6 expression and prognosis using The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and Genotype Tissue Expression (GTEx) data. Subsequently, we used the cytoHubba plug-in of Cytoscape software and starBase to identify the noncoding RNAs (ncRNAs) regulating CDK4/6. Finally, we verified the effect of CDK4/6 on immunity in OC cell lines and animal models.ResultsCDK4/6 expression was higher in OC tissues than in normal ovarian tissues, and the high expression levels of CDK4/6 contributed to the immunosuppressive state of OC and were thus related to the poor prognosis of OC patients. This was also in general agreement with the results of OC cell line and animal experiments. Mechanistically, the CDK4/6 inhibitor palbociclib increased the secretion of interferon (IFN)-γ and the interferon-stimulated gene (ISG) response, thereby upregulating the expression of antigen-presenting molecules; this effect was partly dependent on the STING pathway and thus activated immunity in OC. Additionally, according to public data, the LRRC75A-AS1-hsa-miR-330-5p axis could inhibit the immune response of OC patients by upregulating CDK4/6, leading to a poor prognosis.ConclusionCDK4/6 affects the immune microenvironment of OC and correlates with the prognosis of OC patients.