bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Weight-Related Stigmas With Daily Pain Symptoms Among Individuals With Obesity
Authors: Olson KayLoni L , Panza Emily , Lillis Jason , Wing Rena R 
Published: June 2022

AbstractBackgroundIndividuals with obesity are disproportionately impacted by pain-related symptoms.PurposeThis study evaluated experienced weight stigma and internalized weight bias (IWB) as predictors of pain symptoms in daily life among individuals with obesity.MethodsAdults with obesity (n = 39; 51% female, 67% White, 43.8 ± 11.6 years old, BMI = 36.8 ± 6.7 kg/m2) completed a baseline assessment (demographics, experienced weight stigma, IWB) and a 14-day Ecological Momentary Assessment (EMA) period involving five daily prompts of pain/aches/joint pain, muscle soreness, experienced weight stigma, and IWB. Generalized linear models were used to assess experienced weight stigma and IWB at baseline as prospective predictors of EMA pain/soreness symptoms. Multi-level models were used to test the association of momentary weight stigma experiences and IWB with pain/soreness at the same and subsequent EMA prompts.ResultsIWB at baseline, but not experienced weight stigma, was associated with more frequent pain symptoms (p &lt; .05) and muscle soreness (p &lt; .01) during EMA. Momentary IWB (but not experienced stigma) was associated with more pain/aches/joint pain and muscle soreness at the same and subsequent prompt.ConclusionsInternalized (but not experienced) weight bias was prospectively associated with pain symptoms in daily life among individuals with obesity. Results are consistent with growing evidence that weight-related stigmas represent psychosocial factors that contribute to weight-related morbidity typically attributed to body size.
Coronavirus Disease 2019 (COVID-19) Vaccination Coverage, Intentions, Attitudes, and Barriers by Race/Ethnicity, Language of Interview, and Nativity—National Immunization Survey Adult COVID Module, 22 April 2021–29 January 2022
Authors: Ohlsen Elizabeth C , Yankey David , Pezzi Clelia , Kriss Jennifer L , Lu Peng Jun , Hung Mei Chuan , Bernabe Maria I Dionicio , Kumar Gayathri S , Jentes Emily , Elam-Evans Laurie D , Jackson Hannah , Black Carla L , Singleton James A , Ladva Chandresh N , Abad Neetu , Lainz Alfonso Rodriguez 
Published: June 2022

Abstract
The National Immunization Survey Adult COVID Module used a random-digit-dialed phone survey during 22 April 2021–29 January 2022 to quantify coronavirus disease 2019 (COVID-19) vaccination, intent, attitudes, and barriers by detailed race/ethnicity, interview language, and nativity. Foreign-born respondents overall and within racial/ethnic categories had higher vaccination coverage (80.9%), higher intent to be vaccinated (4.2%), and lower hesitancy toward COVID-19 vaccination (6.0%) than US-born respondents (72.6%, 2.9%, and 15.8%, respectively). Vaccination coverage was significantly lower for certain subcategories of national origin or heritage (eg, Jamaican [68.6%], Haitian [60.7%], Somali [49.0%] in weighted estimates). Respondents interviewed in Spanish had lower vaccination coverage than interviewees in English but higher intent to be vaccinated and lower reluctance. Collection and analysis of nativity, detailed race/ethnicity and language information allow identification of disparities among racial/ethnic subgroups. Vaccination programs could use such information to implement culturally and linguistically appropriate focused interventions among communities with lower vaccination coverage.
PolarX Cryoballoon metrics predicting successful pulmonary vein isolation: targets for ablation of atrial fibrillation
Authors: Honarbakhsh Shohreh , Earley Mark J , Martin Claire A , Creta Antonio , Sohaib Afzal , Ang Richard , Butcher Charles , Waddingham Peter H , Dhinoja Mehul , Lim Wei , Srinivasan Neil T , Providencia Rui , Kanthasamy Vijayabharathy , Sporton Simon , Chow Anthony , Lambiase Pier D , Schilling Richard J , Finlay Malcolm C , Hunter Ross J 
Published: June 2022

Abstract

Aim
Evaluate the novel PolarX Cryoballoon in atrial fibrillation (AF) catheter ablation through a propensity-matched comparison with the Arctic Front Advance (AFA). The aim was also to identify cryoablation metrics that are predictive of successful pulmonary vein isolation (PVI) with the PolarX Cryoballoon.

Methods and results
This prospective multi-centre study included patients that underwent cryoablation for AF. All patients underwent PVI with reconnection assessed after a 30-min waiting period and adenosine. Safety, efficacy, and cryoablation metrics were compared between PolarX and a propensity-matched AFA cohort. Seventy patients were included with 278 veins treated. In total, 359 cryoablations were performed (1.3 ± 0.6 per vein) to achieve initial PVI with 205 (73.7%) veins isolating with a single cryoablation. Independent predictors for achieving initial PVI included temperature at 30 s [odds ratio (OR) 1.26; P = 0.003] and time to reach −40°C (OR 1.88; P &lt; 0.001) with an optimal cut-off of ≤−38.5°C at 30 s [area under the curve (AUC) 0.79; P &lt; 0.001] and ≤−40°C at ≤32.5 s (AUC 0.77; P &lt; 0.001), respectively. Of the 278 veins, 46 (16.5%) veins showed acute reconnection. Temperature at 30 s (≤−39.5°C, OR 1.24; P = 0.002), nadir temperature (≤−53.5°C, OR 1.35; P = 0.003), and time to isolation (≤38.0 s, OR 1.18; P = 0.009) were independent predictors of sustained PVI. Combining two of these three targets was associated with reconnection in only 2–5% of PVs. Efficacy and safety of the PolarX Cryoballoon were comparable to AFA Cryoballoon, however, cryoablation metrics were significantly different.

Conclusions
The PolarX Cryoballoon has a different cryoablation profile to AFA Cryoballoon. Prospective testing of these proposed targets in large outcomes studies is required.
Construction of a high-density bin-map and identification of fruit quality-related quantitative trait loci and functional genes in pear
Authors: Qin Meng-Fan , Li Lei-Ting , Singh Jugpreet , Sun Man-Yi , Bai Bing , Li Si-Wei , Ni Jiang-Ping , Zhang Jia-Ying , Zhang Xun , Wei Wei-Lin , Zhang Ming-Yue , Li Jia-Ming , Qi Kai-Jie , Zhang Shao-Ling , Khan Awais , Wu Jun 
Published: June 2022

Abstract
Pear (Pyrus spp.) is one of the most common fruit crops grown in temperate regions worldwide. Genetic enhancement of fruit quality is a fundamental goal of pear breeding programs. The genetic control of pear fruit quality traits is highly quantitative, and development of high-density genetic maps can facilitate fine-mapping of quantitative trait loci (QTLs) and gene identification. Bin-mapping is a powerful method of constructing high-resolution genetic maps from large-scale genotyping datasets. We performed whole-genome sequencing of pear cultivars ‘Niitaka’ and ‘Hongxiangsu’ and their 176 F1 progeny to identify genome-wide single-nucleotide polymorphism (SNP) markers for constructing a high-density bin-map of pear. This analysis yielded a total of 1.93 million SNPs and a genetic bin-map of 3190 markers spanning 1358.5 cM, with an average adjacent interval of 0.43 cM. This bin-map, along with other high-density genetic maps in pear, improved the reference genome assembly from 75.5 to 83.7% by re-anchoring the scaffolds. A quantitative genetic analysis identified 148 QTLs for 18 fruit-related traits; among them, QTLs for stone cell content, several key monosaccharides, and fruit pulp acids were identified for the first time in pear. A gene expression analysis of six pear cultivars identified 399 candidates in the identified QTL regions, which showed expression specific to fruit developmental stages in pear. Finally, we confirmed the function of PbrtMT1, a tonoplast monosaccharide transporter-related gene responsible for the enhancement of fructose accumulation in pear fruit on linkage group 16, in a transient transformation experiment. This study provides genomic and genetic resources as well as potential candidate genes for fruit quality improvement in pear.
MdERF1B–MdMYC2 module integrates ethylene and jasmonic acid to regulate the biosynthesis of anthocyanin in apple
Authors: Wang Shuo , Li Li-Xian , Fang Yue , Li Dan , Mao Zuolin , Zhu Zihao , Chen Xue-Sen , Feng Shou-Qian 
Published: June 2022

Abstract
Ethylene and jasmonic acid (JA) are crucial hormones that promote anthocyanin synthesis in apple (Malus × domestica). However, the mechanism by which these hormones cooperate to modulate anthocyanin production in apple is unclear. According to our results, MdERF1B expression was strongly induced by ethylene and JA. Physiological phenotypes and the results of molecular biological analyses indicated that MdERF1B encodes a positive regulator of anthocyanin synthesis. Specifically, MdERF1B was capable of combining directly with the MdMYC2 promoter to promote gene expression. Additionally, MdERF1B interacted with two JA signaling pathway inhibitors, namely MdJAZ5 and MdJAZ10. The MdERF1B–MdJAZ5/10 protein complex decreased the ability of MdERF1B to activate the MdMYC2 promoter. Furthermore, MdEIL1, which is a crucial protein for ethylene signal transduction, was observed to bind directly to the MdERF1B promoter, thereby upregulating gene expression. These results suggest that MdERF1B is a core gene responsive to JA and ethylene signals. The encoded protein, together with MdMYC2, MdJAZ5/10, and MdEIL1, modulates anthocyanin synthesis in apple. This study clarifies the synergistic mechanism by which JA and ethylene regulate anthocyanin production in apple.
New methods for quantification of amoxicillin and clindamycin in human plasma using HPLC with UV detection
Authors: Greibe Eva , Moser Claus Ernst , Bruun Niels Eske , Hoffmann-Lücke Elke 
Published: June 2022

Abstract

Objectives
We aimed to develop simple and rapid HPLC methods for determination of amoxicillin and clindamycin in human plasma.

Methods
Plasma samples were pretreated by direct deproteinization with acetonitrile and the analytical separation took place on a reverse phase Poroshell 120 EC-C18 column (2.7 μm, 2.1 × 100 mm) with a gradient of acetonitrile. UV detection at 229 nm for amoxicillin and 204 nm for clindamycin was used for determination of the antibiotics in plasma.

Results
The calibration curves were linear over the concentration ranges of 1–100 mg/L for amoxicillin and 1–15 mg/L for clindamycin with a correlation coefficient of ≥0.98. Intra-assay precisions were all ≤15% and the accuracies were within ±15%. The limit of quantification (LOQ) was found to be 0.5 mg/L for amoxicillin and 1 mg/L for clindamycin with inter-assay imprecision coefficient of variances (CVs) of 18.7% and 15.6%, respectively. The present HPLC methods were successfully applied on spike-in samples and on plasma samples collected 4–6 and 3.5–5.5 h after oral antibiotic administration of 500 mg of amoxicillin and 600 mg of clindamycin, respectively.

Conclusions
We have developed HPLC methods with UV detection for quantification of amoxicillin and clindamycin in human plasma. The methods are fast, simple and suitable for use in routine settings and clinical studies.
The Function of Nilaparvata lugens (Hemiptera: Delphacidae) E74 and Its Interaction With βFtz-F1
Authors: Zhang Yuwei , Zheng Shiwen , Li Yan , Jiang Xiaojuan , Gao Han , Lin Xinda 
Published: June 2022

Abstract
Drosophila E74 is an early gene located in the polytene chromosome 74EF puff position. E74 controls the production of late genes, indicating that it plays a crucial role in this cascade model. Nilaparvata lugens E74 is closely related to Diaphorina citri, Bemisia tabaci, and Laodelphax striatellus. After downregulating E74, molting, and nymphal mortality were increased, and ovarian development was delayed. Moreover, the expression of Vg was reduced at the transcriptional level, as measured by qRT-PCR, and the content of Vg protein was reduced, as detected by Western blotting. After downregulating E74, the expression of hormone-related genes, including Tai, βFtz-F1, Met, Kr-h1, UspA, UspB, E93, and Br, was changed. The expression of E74 was significantly decreased after downregulating hormone-related genes. When the expression of E74 and βFtz-F1 was downregulated together, nymph mortality and molting mortality were higher than those when E74 or βFtz-F1 was downregulated alone. Thus, E74 probably interacts with βFtz-F1 at the genetic level. In summary, this study showed that E74 plays a crucial role in the development, metamorphosis and reproduction of N. lugens, possibly via the interaction with βFtz-F1 at the genetic level. This study provides a basis for the development of new target-based pesticides and new methods for the effective control of N. lugens.
Parentage Assignment Using Microsatellites Reveals Multiple Mating inAedes aegypti(Diptera: Culicidae): Implications for Mating Dynamics
Authors: Pimid Marcela , Krishnan Kumara Thevan , Ahmad Abu Hassan , Mohd Naim Darlina , Chambers Geoffrey K , Mohd Nor Siti Azizah , Ab Majid Abdul Hafiz 
Published: June 2022

AbstractThe mosquito Aedes aegypti is the primary vector of the dengue, yellow fever, and chikungunya viruses. Evidence shows that Ae. aegypti males are polyandrous whereas Ae. aegypti females are monandrous in mating. However, the degree to which Ae. aegypti males and females can mate with different partners has not been rigorously tested. Therefore, this study examined the rates of polyandry via parentage assignment in three sets of competitive mating experiments using wild-type male and female Ae. aegypti. Parentage assignment was monitored using nine microsatellite DNA markers. All Ae. aegypti offspring were successfully assigned to parents with 80% or 95% confidence using CERVUS software. The results showed that both male and female Ae. aegypti mated with up to 3–4 different partners. Adults contributed differentially to the emergent offspring, with reproductive outputs ranging from 1 to 25 viable progeny. This study demonstrates a new perspective on the capabilities of male and female Ae. aegypti in mating. These findings are significant because successful deployment of reproductive control methods using genetic modification or sterile Ae. aegypti must consider the following criteria regarding their mating fitness: 1) choosing Ae. aegypti males that can mate with many different females; 2) testing how transformed Ae. aegypti male perform with polyandrous females; and 3) prioritizing the selection of polyandrous males and/or females Ae. aegypti that have the most offspring.
Grounding
Authors: Schielke Hugo J. , Brand Bethany L. , Lanius Ruth A. 
Published: June 2022

Abstract
Grounding offers powerful help toward managing and reducing symptoms related to trauma, including feeling “too much” (feeling strong or overwhelming feelings or urges); feeling “too little” (feeling disconnected or numb); feeling confused or having a hard time thinking clearly; losing track of time or where you are; having a hard time noticing when things are safer; or feeling like the past is happening again. This module will help you learn and practice the two core grounding skills (orienting to and anchoring in the present). In addition, the module describes how to notice you are getting ungrounded and offers 101 ways to get and stay grounded so you can find the ways of grounding that work best for you. By doing this work, you can learn to prevent yourself from getting overwhelmed by developing healthy, healing-focused ways to manage when you start to feel too much or too little.
Separating Past from Present
Authors: Schielke Hugo J. , Brand Bethany L. , Lanius Ruth A. 
Published: June 2022

Abstract
Trying to keep us safer, our minds are often on alert for signs of danger. Unfortunately, because we notice similarities with past experiences far easier than differences, this means we can have significant difficulty noticing when situations are safer. In other words, we can have difficulty separating the past from the present. This module offers ways to help yourself notice when the present is safer than the past and be more aware of your current resources and options. It will also help you learn how to catch thinking mistakes that can make it difficult to separate past from present, imagery techniques that can help you separate the present from the past, and how to contain traumatic intrusions, all of which will help you see the “here and now” more clearly, making it easier to stay grounded, heal, and grow.
Additional Foundations
Authors: Schielke Hugo J. , Brand Bethany L. , Lanius Ruth A. 
Published: June 2022

Abstract
This module offers ways to help yourself make progress toward getting and feeling safer. This module focuses on teaching you things you can do toward healing the impact of trauma, and steps you can expect along the way. As part of this, you will learn how to notice signs you might be having trauma-related thoughts, and how to manage them so that past experiences do not automatically make you feel bad. This module will also help you develop and use plans for managing intense emotions, noticing and managing trauma-based thoughts, and developing and using imagery techniques for when you’re feeling too much.
Addressing Trauma-Based Thinking
Authors: Schielke Hugo J. , Brand Bethany L. , Lanius Ruth A. 
Published: June 2022

Abstract
This module focuses on learning how to identify and reduce trauma-based thinking, which is often unfair or distorted but feels true due to trauma. This module will discuss trauma-based thoughts as a common symptom of having experienced trauma and will help you learn how to reduce trauma-based thoughts. It will help you practice identifying trauma-based thoughts, noticing evidence that challenges trauma-based thoughts. It will also help you identify and generate healing-focused thoughts and work toward shifting from trauma-based thoughts to healing-focused thinking. This difficult, important work builds on all you’ve already done as part of this program. Taking the time to do the work involved in reducing trauma-based thoughts can go a long way toward getting and feeling safer.
Improving Your Relationship with Emotions, Body Sensations, and Aspects of Self
Authors: Schielke Hugo J. , Brand Bethany L. , Lanius Ruth A. 
Published: June 2022

Abstract
Trauma can dramatically change the range of emotions and physical sensations that feel comfortable or manageable. People who have experienced trauma often feel too much or too little emotion and body sensations. Traumatic experiences can lead people to believe that certain feelings are not OK—or that no emotions are OK. This can lead people to not want their emotions and body sensations and to be afraid of emotions and sensations. In addition, they may never have learned how to have a healthy relationship with their emotions, body sensations, or themselves as a person. At the same time, it is difficult to get and feel safer without a healthy relationship with your emotions, body, and all of who you are. This module will help you work toward manageably developing healthy relationships with these aspects of your experience. This will gradually help you get and feel safer.
What Is Consciousness?
Authors: McVeigh Brian J. 
Published: June 2022

AbstractThis chapter contends that for clinical purposes, tidying up the idiom of mentation will go a long way in helping discern how various interventions relate, cohere, and operate. This chapter, in order to contribute to clearing the theoretical thicket, proposes that what is vaguely called consciousness can be broken down into FOCI. Since much of one’s mental healing transpires on the back of consciousness, it behooves one to comprehend what consciousness signifies. This chapter, then, clarifies the meaning of conscious interiority. This chapter also treats “underground” and “background” psychological processes and four types of nonconsciousness; consciousness as a type of meta-framing; the creative potential of language to construct cognition; metaphors as meta-framing; and the therapeutic use of metaphors.
Runaway Consciousness
Authors: McVeigh Brian J. 
Published: June 2022

AbstractThis chapter explores how conscious interiority, which ordinarily is a benefit, can go awry. Under certain circumstances, e.g., if life experiences or innate vulnerabilities overwhelm a person, FOCI can lead to runaway consciousness. Consequently, FOCI end up hindering mental health and mutate into psychological processes in need of modification themselves. And yet FOCI themselves can be deployed as therapeutic techniques to correct distorted cognition and hyper-interiorization (i.e., the therapeutically directed uses of FOCI). This chapter also explores the role of resistance and how individual self-deception and institutional mendacity interlock. Three types of consciousness are distinguished: everyday, therapeutic, and excessive or hyper-interiorized.
Using Conscious Interiority as a Therapeutic Tool
Authors: McVeigh Brian J. 
Published: June 2022

AbstractThis chapter returns to the particular systems of psychotherapy (introduced in Chapter 5). However, in this chapter the interventions and techniques of the various systems are examined to illustrate how they incorporate FOCI for healing purposes. In other words, conscious interiority is shown to operate as a therapeutic tool in the different psychotherapeutic systems, thereby bolstering the common factors and integrative psychological approach of this book. This is accomplished by examining how hyper-interiority is corrected by utilizing FOCI; in rational emotive behavior therapy (disputing irrational notions via active-directive approach); cognitive behavior therapy (identifying and learning maladaptive behaviors); client-centered therapy (highlighting the interiorized, subjective contents of mind); existential therapy (confronting our self-individuation); Gestalt therapy (focusing on the experiential self); dialectical behavior therapy (balancing the need for change with the way things are); acceptance and commitment therapy (coming to terms with one’s circumstances and enacting one’s values). Also examined are the A-B-C-D chain of cognition, FOCI and self-improvement, and self-change through self-reflexivity.
Groups and Families as Therapeutic Systems
Authors: McVeigh Brian J. 
Published: June 2022

AbstractThis chapter examines the processual, transitional, and systems-theoretical facets of group and family therapies while highlighting how they relate to various FOCI. Groups and families are not just aggregates of individuals who happen to end up together; they need to be conceived as emotionally-charged systems whose interactive dynamics can be perceived as transitioning through distinct and discernible stages. Also explored are the dynamics of positive change and transformation via communitas and cohesiveness; transference and countertransference as elements of transformation; group facilitators as stage directors; different approaches to family therapy; communication and information flow within the family; and parenting styles and conscious interiority.
Anatomy of four human Argonaute proteins
Authors: Nakanishi Kotaro 
Published: June 2022

Abstract
MicroRNAs (miRNAs) bind to complementary target RNAs and regulate their gene expression post-transcriptionally. These non-coding regulatory RNAs become functional after loading into Argonaute (AGO) proteins to form the effector complexes. Humans have four AGO proteins, AGO1, AGO2, AGO3 and AGO4, which share a high sequence identity. Since most miRNAs are found across the four AGOs, it has been thought that they work redundantly, and AGO2 has been heavily studied as the exemplified human paralog. Nevertheless, an increasing number of studies have found that the other paralogs play unique roles in various biological processes and diseases. In the last decade, the structural study of the four AGOs has provided the field with solid structural bases. This review exploits the completed structural catalog to describe common features and differences in target specificity across the four AGOs.