bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Integrative Analysis of Competitive Endogenous RNA Regulatory Networks in Osteoporosis
Authors: Sun Huijun , Li Hao , Wang Changyuan , Jin Yue , Cai Yuanqing , Liu Mozhen 
Published: February 2022

Abstract
Background: Osteoporosis (OP) is a common bone disease of old age resulting from the imbalance between bone resorption and bone formation. circRNAs are a class of endogenous noncoding RNAs (ncRNAs) involved in gene regulation and may play important roles in the development of OP. Here, we aimed to discover the OP‑related circRNA-miRNA-mRNA (ceRNA) network and the potential mechanisms.Results: Six datasets were obtained from GEO databases and the differentially expressed genes, circRNAs, and miRNAs were screened out from these datasets. Then, combined with the predicted target miRNAs and target genes, a total of 7 target circRNAs, 5 target miRNAs, 38 target genes, and a ceRNA network was constructed. Then RNA-seq verification by using total RNA isolated from the femurs of normal and oophorectomy Wistar rats indicated that MFAP5, CAMK2A, and RGS4 were closely associated with osteoporosis. Function enrichment analysis indicated that the target circRNAs, miRNAs, and genes were involved in the process of MAPK cascade, hormone stimulus, cadherin binding, rRNA methyltransferase, PI3K-Akt signaling pathway, and Vitamin digestion and absorption, et.al. Then a circRNA-miRNA-hub gene subnetwork was constructed and the RT-qPCR analysis of human bone tissues from the femoral head was used to confirm that the transcription of hsa_circR_0028877, hsa_circR_0082916, DIRAS2, CAMK2A, and MAPK4 showed a significant correlation with osteogenic genes. Besides, the two axes of hsa_circR_0028877 - hsa-miR-1273f - CAMK2A and hsa_circR_0028877 - hsa-miR-1273f - DIRAS2 conformed to be closely associated with OP. Additionally, by constructing a drug-target gene network, RKI-1447, FRAX486, Hyaluronic, and Fostamatinib were identified as therapeutic options for OP.Conclusions: Our study revealed the potential links between circRNAs, miRNAs, and mRNAs in OP, suggesting that the ceRNA mechanism may contribute to the occurrence of OP.
Bcl-2 and Noxa are potential prognostic indicators for patients with gastroenteropancreatic neuroendocrine neoplasms
Authors: Yu Guo , Lin Zhang , Ning Zhang , Qiuyun Luo , Man Liu , Dajun Yang , Chen Jie 
Published: February 2022

Abstract
Purpose Bcl-2 family of proteins are of great significance in the pathogenesis and development of tumor. In this study, the relationship between the expression of Bcl-2 family proteins and clinicopathological features and prognosis of NENs were further investigated. Methods 105 Patients diagnosed with gastroenteropancreatic NENs (GEP-NENs) with the paraffin specimen of the tumor available were retrospectively included, immunohistochemistry (IHC) was performed to detect the expression of Bcl-2 family proteins in paraffin-embedded samples. Student’s t test and Chi-square test were applied to compare the difference of quantitative and categorical variables, respectively. Prognosis analysis was conducted according to Kaplan⁃Meier method. Univariate COX and multivariate COX regression analysis were used to screen out the independent prognostic factors. Results The IHC score of Bcl-2 was significant higher in neuroendocrine carcinomas (NECs) patients (65.6% vs. 38.4%, P = 0.010), while higher IHC score of Noxa was more common in neuroendocrine tumors (NETs) patients (49.3% vs. 25.0%, P = 0.020). Survival analysis suggested that patients with higher Bcl-2 expression and lower Noxa expression had worse survival rate (42.9% vs. 73.2%, P < 0.001; 42.6% vs. 81.8%, P < 0.001). Multivariate cox analysis indicated that high Bcl-2 expression was an independent factor associated with poor DFS (hazard ratio [HR]: 2.661; 95% confidence interval [CI]: 1.385–5.110) and poor OS (HR: 3.205; 95% CI: 1.587–6.473; P = 0.001), while higher Noxa expression was associated with improved DFS (HR:0.324; 95% CI: 0.147–0.713) and better OS (HR: 0.293; 95% CI: 0.126–0.684). Conclusion Higher expression of Bcl-2, and lower expression of Noxa were associated with unfavorable prognosis of GEP-NEN patients.
Frequency Distribution Of ABO Alleles In A Malaria-Endemic Area Of Pakistan
Authors: Rasheed Seher , Moiz Bushra 
Published: February 2022

Abstract
Background: Falciparum malaria has exerted adaptive responses in mankind over a period of years for their survival advantage. Red cell disorders like thalassemia, Glucose 6 phosphate dehydrogenase deficiency, sickle cell anemia parallel the endemicity of malaria and are associated with malaria resistance. Distribution of ABO types had been linked to the prevalence of malaria. Pakistan has a huge burden of malaria, and the commonly reported blood phenotype is O. Despite this, no local information is available for allelic frequency of blood types. The study aimed in determining the frequency of ABO blood group alleles in a malaria-endemic area of Pakistan.Methods: A total of 90 blood samples from healthy blood donors residing in Southern Pakistan were included in this study. Serological testing was performed using gel column agglutination and the conventional tube method. DNA extraction was performed from whole blood using Extra-gene I kit) followed by molecular genotyping by polymerase chain reaction, using sequence-specific primers (SSP-PCR) BAGene ABO kits. Results: Of 90 samples, O phenotype constituted 33.3% (n=30) of the total ABO types. Twelve genotypes were identified in a combination of 6 common ABO alleles. Allele frequency of ABO*O.01.01 was highest at 0.4944 followed by ABO*B.01 (allelic frequency of 0.2555) and ABO*A1.01 (allelic frequency 0.1666). Conclusion: This study demonstrated the ABO*O.01.01 as the most frequent ABO allele in a malaria-endemic area at Pakistan and may be considered as an adaptive response to malaria. We need large scale studies for determining ABO alleles in different regions of the country depending on variability in malaria transmission.
Roseicella aquatilis sp. nov., isolated from freshwater lake
Authors: du zongjun , Zhang Dandan , Zhao Ya-Nan , He Rui-Han , Yan Yu-Qi 
Published: February 2022

Abstract
A novel Gram-stain-negative, non-motile, ellipsoidal-shaped, red-pigmented, facultatively aerobic strain designated NE82T, was isolated from mud sample from Jiugongli Lake in Inner Mongolia Autonomous Region, China. Optimal growth occurred at 28–33°C (range 15–42°C) and pH 7.0-7.5 (range 5.5–8.5) with 0% (w/v) NaCl (range 0–1.0%). Cells of strain NE82T were 0.4–0.9 µm in diameter, catalase-positive and oxidase-negative. Q-10 was the sole respiratory quinone and the major cellular fatty acids (> 10%) in strain NE82T were summed feature 8 (C18:1 ω7c and C18:1 ω6c). The polar lipids of strain NE82T were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, an unidentified aminophospholipid and four unidentified phospholipids. The G + C content of the genomic DNA was 72.0 mol%. Based on the 16S rRNA gene sequence, strain NE82T, showed the highest similarity (97.2%) to Roseicella frigidaeris DB1506T within the family Acetobacteraceae, represents a novel species of the genus Roseicella, for which the name Roseicella aquatilis sp. nov. is proposed. The type strain is NE82T (=KCTC 62412T༝MCCC 1H00292T).
Artemisia Annua Extracts Improve the Cognitive Deficits and Reverse the Pathological Changes of Alzheimer’s Disease via Regulating YAP Signaling
Authors: Zhou Wenshu , Lei Bingxi , Yang Chao , Silva Marta , Xing Xingan , Zheng Wenhua 
Published: February 2022

Abstract
Background: Alzheimer’s disease (AD) is a chronic neurodegenerative disease characterized by a progressive loss of memory and cognitive functions, language disorders, functional and behavioral alterations. The neurotoxicity is associated with the aggregation of β-amyloid (Aβ) plaques and the downstream pathologies events such as Tau hyperphosphorylation, oxidative stress, neuroinflammation, and mitochondrial dysregulation play critical roles in the development of AD. With no effective treatment available for the prevention and treatment of AD, the search for new therapies has become the focus of many researchers.Methods: Artemisia annua extracts were extracted and a water-soluble artemisia annua extract (Ex1) was used in treatment for 3xTg AD mice via oral administration. Cognitive functional recovery, Aβ accumulation, hyper-tau-phosphorylation, and the release of inflammatory factors and apoptosis were assessed three months after the treatment. In vitro, PC12 cells were incubated with 8 μM Aβ1-42 with or without Ex1 at different concentrations for 24h. ROS levels, mitochondrial membrane potential, caspase-3 activity, neuronal cell apoptosis, inflammation, and the phosphorylation of Tau, as well as involvement in the signaling pathway, were assessed by using biological technology. Results: Oral administration of Ex1 to AD 3xTg mice significantly improved the cognitive deficits, reduced Aβ accumulation, hyper-tau-phosphorylation, and the release of inflammatory factors and apoptosis. Ex1 promoted the survival and proliferation of neural progenitor cells (NPS), increased the expression of synaptic proteins and neuronal cell survival. Similarly, Ex1 significantly reversed the Aβ1-42-induced increase of ROS levels, caspase-3 activity, neuronal cell apoptosis, inflammation, and the phosphorylation of tau in vitro.Conclusions: These findings suggest that a water-soluble artemisia annua extract (Ex1) may be a new multi-target anti-AD drug with potential use in the prevention and treatment of Alzheimer’s disease.
System GMM-based Model for Monitoring Joint Impact of ICT-infrastructure, Financial Development, and Trade-openness on Economic-growth
Authors: Kumari Reenu , Singh Sunil Kumar 
Published: February 2022

Abstract
This study has analyzed the joint impact of information communications technology (ICT) Infrastructure, financial development (FD), and trade openness (TO) on economic growth (EG). We have used the data from 85 countries (including 27 low-income and 58 high-income countries). In this sample, we have collected the data from the year 2000-2019. We have framed hypotheses for samples and applied OLS, fixed effect regression (FER), and GMM method. Our results provide evidence that (a) ICT infrastructure is a significant and positive interpreter of economic growth, (b) Individual consideration of trade openness and financial development is insignificant and negative for both groups of countries, ICT infrastructure requires more financial development and trade openness in low-income countries (LICs) in comparison to high-income countries(HICs), (c) ICT infrastructure-financial development-trade openness nexus differ significantly for both groups of countries. Our study acquiesces that variables are grave drivers of any economy and comprehensive growth in low-income countries. In this work, we have also discussed policy implications.
Distinct Genomic Features Between Osteosarcomas Firstly Metastasing to Bone and to Lung
Authors: Xie Lu , Cai Zhenyu , Lu Hezhe , Meng Fanfei , Zhang Xin , Luo Kun , Su Xiaoxing , Lei Yan , Xu Jiuhui , Lou Jingbing , Wang Han , Du Zhiye , Wang Yunfan , Li Yuan , Ren Tingting , Xu Jie , Sun Xin , Tang Xiaodong , Guo Wei 
Published: February 2022

Abstract
Background: Osteosarcoma (OS) initially metastasing to bone only shows distinct biological features compared to osteosarcoma that firstly metastasizes to the lung, which suggests us underlying different genomic pathogenetic mechanism. However related-study is lacking.Methods: We analyzed whole-exome sequencing (WES) data for 38 metastatic OS patients with different relapse patterns. Multiplex Immunohistochemistry was also applied to investigate the immune- microenvironment. We tried to redefine disease subclassifications for OS based on genetic alterations to guide treatment strategies, and we correlated these genetic profiles with clinical treatment courses and prognosis to elucidate potential evolving cladograms.Results: We investigated whole-exome sequencing of 12/38 patients with high-grade OS (31.6%) with initial bone metastasis (group A) and 26/38 (68.4%) with initial pulmonary metastasis (group B), of whom 15/38 (39.5%) had paired samples of primary lesions and metastatic lesions for these two cohorts with different relapse patterns. We found that osteosarcoma in group A mainly carries single-nucleotide variations while those in group B mainly exhibits structural variants. Additionally, osteosarcoma in group A displayed better immunogenicity, including a higher tumor mutation burden and neoantigen load and more tertiary lymphoid structures in tumor microenvironment.Conclusions: Osteosarcoma with different relapse patterns has distinct genomic landscapes. This study suggests that osteosarcoma with mainly single-nucleotide variations other than structural variants might exhibit biological behavior predisposing toward bone metastases; immunotherapy is a promising therapeutic option for this group of patients.Trial registrationThis trial was registered in the Medical Ethics Committee of Peking University People’s Hospital on June 25th, 2019 (Institutional Review Board, IRB, number of 2019PHB139-01). The trial was registered in Clinicaltrials.gov with identifier no. NCT03997747.
Deep Multilayer Perceptron Wind Speed Estimation Based Artificial Immune System For Power Generation Prediction
Authors: T Vinoth Kumar , N Deepa S , S Tamizharasi , N Yogambal Jayalashmi 
Published: February 2022

Abstract
Wind energy is fast developing energy resource as it is renewable, pollution free and abundant. The nonlinear and fluctuation of wind are large demand for enhancing the reliability and accuracy of the power system that combines the wind speed. With an exact wind speed data, power system operators predict the power output for system planning and scheduling. Wind speed prediction is an essential factor in forecasting to attain the efficient wind power utilization. But, the prediction time and accuracy performance was not improved using existing techniques. In order to address this problem, Deep Multilayer Perceptron Neural Network based Clonal Selective Optimization (DMLPNN-CSO) technique is introduced. The main objective of DMLPNN-CSO technique is to improve the performance of power generation prediction through wind speed estimation with higher accuracy based on mean air temperature, relative humidity and vapor pressure data. DMLPNN-CSO technique comprises two processes, namely deep multilayer perceptron neural network and clonal data selection algorithm. Initially in DMLPNN-CSO technique, wind turbine is considered as the input and given to the input layer in deep multilayer perceptron neural network. Wind turbine information is extracted and given to the hidden layer 1. In this layer, data preprocessing is performed through the median filter for removing unnecessary data. After that, the preprocessed data is given to the hidden layer 2 to estimate the wind speed. The estimated values are collected and get matched with the pre-stored values in the output layer by using softsign activation function. After estimation of wind speed, the optimal data gets selected by using clonal data selection algorithm for predicting the power generation. Clonal data selection algorithm performs the sorting, cloning and guassian mutation for finding the optimal value in power generation prediction. This in turn helps to improve prediction accuracy and reduce the time consumption. Experimental evaluation of proposed DMLPNN-CSO technique is carried out with respect to number of wind turbine data and data samples. The results showed that DMLPNN-CSO technique produces higher prediction accuracy and wind speed estimation rate when compared to state-of-the-art works.
Fixed Broadband Take-Up in Europe
Authors: Leogrande Angelo , Magaletti Nicola , Cosoli Gabriele , Massaro Alessandro 
Published: February 2022

Abstract
In this article the value of “Fixed Broadband Take Up” in Europe is investigated. Data are collected from the DESI-Digital Economy and Society Index for 28 countries in the period 2016-2021. Data are analyzed with Panel Data with Fixed Effects and Random Effects. The Fixed Broadband Take-Up value is positively associated with the value of "Connectivity", "Human Capital", "Desi Index", "Fast BB NGA Coverage", "Fixed Very High-Capacity Network VHCN coverage". Fixed Broadband Take-Up value is negatively associated with "Digital Public Services for Businesses", "e-Government", "At least Basic Digital Skills", "At Least Basic Software Skills", "Above Basic Digital Skills", "Advanced Skills and Development", "Integration of Digital Technology", "Broadband Price Index", "Mobile Broadband", "Fixed Broadband Coverage". Subsequently the k-Means algorithm optimized by the Silhouette coefficient was used to identify the number of clusters. The analysis shows the presence of the two clusters. Eight different machine learning algorithms were then used to predict the future value of the "Fixed Broadband Take-Up in Europe". The analysis shows that the most efficient algorithm for the prediction is "ANN-Artificial Neural Network" with an estimated value of the prediction equal to 26.39%.
SEGURANÇA DA EQUIPE DE SAÚDE X SEGURANÇA DO PACIENTE EM TEMPOS DE PANDEMIA
Authors: Rodrigues da Silva Adriana 
Published: February 2022

Objetivo: Compreender as estratégias para assegurar a segurança das equipes de saúde e do paciente em tempos de pandemia. Método: trata-se de uma revisão integrativa de literatura, desenvolvida no mês de agosto de 2021 através de dados coletados nas bases de dados SciELO; LILACS e BDENF; incluindo sites organizacionais como o Ministério da Saúde, OMS e a OPAS. Fundamentação Teórica: A pandemia COVID-19 trouxe diversos impactos que afetam a saúde ocupacional e do paciente, dentre eles os milhões de óbitos e o comprometimento mental, social e emocional proporcionada por uma emergência em saúde. Resultados: a amostra consistiu em 06 artigos entre os anos de 2020 e 2021, que abordam a temática de segurança do paciente e do profissional de saúde, além da importância do gestor no enfrentamento das dificuldades perante a pandemia COVID-19. Conclusão: evidenciou-se que o cuidado direcionado à saúde do trabalhador é essencial para a promoção e manutenção da segurança e na garantia da qualidade do serviço, sendo a atuação do gestor essencial nesse processo.
AGENDAMENTO EM CENTROS CIRÚRGICOS: UMA SOLUÇÃO BASEADA NA TEORIA DA CORRENTE CRÍTICA
Authors: Flávio Castro Robson , Liguori Zacchi Marcelo , De Castro Moura Duarte André Luís , Meyer Sanches Lars 
Published: February 2022

Objetivo: A pesquisa tem dois objetivos, o primeiro é entender a dinâmica e as razões dos atrasos em um centro cirúrgico (CC) de um hospital de referência, localizado na cidade de São Paulo, e o segundo é propor novas políticas para o processo de agendamento de centros cirúrgicos, baseado na Teoria da Corrente Crítica (TCC).
Método: Para alcançar tais objetivos, foram utilizados o método de Estudo de Caso (YIN, 2014) e a técnica de Simulação de Eventos Discretos. No estudo de caso, foram coletados indicadores de processos, além da realização de entrevistas com gestores de todas as áreas e serviços relacionados ao CC.
Fundamentação teórica: A gestão de centros cirúrgicos é crítica em muitos hospitais, não somente pelos altos custos envolvidos, mas também pela sua complexidade. Os gestores dos CCs são desafiados a reduzirem custos e buscarem melhorias na qualidade dos serviços prestados a pacientes e cirurgiões. A gestão de um CC envolve lidar com incertezas e variabilidades relacionadas com a quantidade e com a duração de cirurgias em um hospital. O processo de agendamento das cirurgias é crucial para o sucesso desta gestão.
Resultados: Os resultados da simulação mostram que o uso da TCC melhorou significativamente os indicadores do centro cirúrgico estudado. Mesmo mantendo o volume de cirurgias por dia, observou-se uma queda no número de cirurgias iniciando com atrasos, de 94,5% para 58,3%. O atraso médio caiu de 54' para 35', uma redução de 35%.
Conclusões e contribuições: Este trabalho procura propor soluções para gestores hospitalares com potencial efeito na redução de desperdícios nas operações dos CCs, na melhora dos indicadores de qualidade e na satisfação dos atores dos CCs e, por consequência, no aumento dos ganhos financeiros das instituições hospitalares.
Autopublicação: o autor como editor no novo mundo editorial
Authors: Morgado Marta 
Published: February 2022

A autopublicação tem uma má reputação no mundo literário e editorial, mas tem crescido cada vez mais durante a era da Internet. Desde a invenção da imprensa que a edição de autor se desenvolveu, mudando a forma como vemos um autor e um editor. As responsabilidades e tarefas de ambos começaram a fundir-se. Este artigo explica o que é a autopublicação e os vários tipos que existem; o porquê dos autores recorrerem a ela; finalizando com os papéis que o autor começou a ter.
Behind the Mask of Virtue: CSR Indocement's Original Face in the Conflict of Cement Factory Construction in Pati
Authors: Novianto Arif , Effendi Kurnia Cahyaningrum , Purbokusumo Yuyun 
Published: February 2022

Discourses about Corporate Social Responsibility (CSR) are generally understood as goodwill and social virtue. This research with a CSR Indocement case study in the Pati District found something different in the study in areas that have not been affected by and in the process of expansion from PT SMS (a subsidiary of PT Indocement Tunggal Prakarsa). CSR Indocement, which has been run in Pati since 2012, is not based on a business ethic or corporate social virtue model. With political economy analysis, we found that 1) CSR Indocement runs in Pati as an effort to expedite the expansion of their capital which has strong resistance from the community rejecting the cement factory; 2) CSR Indocement for the last five years in Pati, nearly 90 programs that have been attempted none have been able to empower the community. These efforts were influenced by two things: 1) In carrying out its program CSR Indocement brought the program to the village elites. 2) CSR Indocement's initial goal for Pati was to build a consensus to give a red carpet for capital expansion so that the goal of empowerment is not paramount.
Arqueología de la mirada
Authors: Keldjian Julieta 
Published: February 2022

Reseña sobre el X Seminario de Archivos Fílmicos: recuperar, identificar, catalogar, restaurar y difundir el cine producido antes de 1920. La Coruña, del 15 al 17 de noviembre de 2006. Centro Galego das Artes da Imaxe (CGAI) de La Coruña.
“Esencialmente sigo siendo periodista”. Entrevista a Daniela Santos
Authors: Caballero Soledad 
Published: February 2022

Daniela Santos hace cinco años que vive en España, y diecisiete que es periodista. Integra el staff de editores de Loft Publications, una editorial especializada en arquitectura y diseño, establecida en Barcelona, cuyos libros se distribuyen en todo el mundo.
Texto de Rosario Lázaro. Amplio
Authors: Lázaro Rosario 
Published: February 2022

Amplio y fuerte era el futuro hasta hace poco. Ahora es fluctuante, pienso, y me calzo las zapatillas de correr. Primero la derecha, que tiene el borde gastado, un registro de kilómetros o un descuido inoportuno.