bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐term outcomes of laparoscopic versus open liver resection for intrahepatic combined hepatocellular‐cholangiocarcinoma with propensity score matching
Authors: Lee Seung Jae , Kang So Hyun , Choi YoungRok , Lee Boram , Hong Suk Kyun , Cho Jai Young , Yi Nam‐Joon , Lee Kwang‐Woong , Suh Kyung‐Suk , Han Ho‐Seong 
Published: February 2022

AbstractBackgroundCombined hepatocellular‐cholangiocarcinoma (cHCC‐CCA) is a rare primary hepatic neoplasm. Currently, there are no well‐structured studies that analyze the feasibility of laparoscopic liver resection in cHCC‐CCA alone. This retrospective cohort study aimed to compare the long‐term survival of laparoscopic liver resection with open liver resection in cHCC‐CCA.MethodsPatients with a postoperative pathologic report of cHCC‐CCA who underwent liver resection from August 2004 to December 2017 were included in this study. Kaplan–Meier survival analysis was performed to analyze the 3‐y disease‐free survival and 3‐y overall survival. Propensity score matching was done to reduce the influence of confounding variables.ResultsA total of 145 patients were pathologically confirmed to have cHCC‐CCA, of which 10 patients were excluded due to having received palliative surgery. Of the remaining 135 patients, 43 underwent laparoscopic and 92 underwent open liver resection; propensity score matching yielded 30 patients for each group. The 3‐y overall survival was 38 (88.4%) in the laparoscopic group and 84 (91.3%) in the open group before propensity score matching (P = .678), and 25 (83.3%) and 28 (93.3%), respectively, after matching (P = .257). The 3‐y disease‐free survival was 24 (55.8%) in the laparoscopic group and 32 (34.8%) in the open group before matching (P = .040), and 17 (56.7%) and 16 (53.3%), respectively, after matching (P = .958). The hospital stay was shorter in the laparoscopic group before and after matching, while other operative outcomes were similar in both groups.ConclusionLaparoscopic liver resection for cHCC‐CCA is technically feasible and safe, having a shorter hospital stay without compromising oncological outcomes.
FeCl3‐Catalyzed Highly Diastereoselective Synthesis of Polyhydrobenzo[a]acridinones
Authors: Wang Zongkang , Qin Zhengchen , Yang Yajie , Jin Shuxin , Bao Fengyu , Li Yanzhong 
Published: February 2022

AbstractFeCl3‐catalyzed diastereospecific synthesis of tetrahydrobenzo[a]acridin‐5(6H)‐ones from enaminones was described. Single diastereoisomers were obtained in good to excellent yields. Two C−C bonds and three contiguous stereogenic centers were formed.
Unique volatile metabolite signature of sinonasal inverted papilloma detectable in plasma and nasal secretions
Authors: Chaskes Mark B. , Lee Young Eun , Toskala Elina , Nyquist Gurston , Rosen Marc , Kimball Bruce , Rabinowitz Mindy 
Published: February 2022

AbstractBackgroundSinonasal inverted papilloma (SNIP) is a benign neoplasm with aggressive features, including a high recurrence rate and a propensity for malignant transformation. Accurate diagnosis with complete resection and the need for close long‐term surveillance is widely accepted as standard management. In this study, we investigate whether SNIP produces a unique volatile metabolite signature, which may ultimately lead to a novel approach to diagnose and monitor SNIP.MethodsWhole blood and nasal secretions from patients with SNIP and healthy age‐, sex‐, and smoking‐status‐matched controls, were collected. There were 56 blood samples and 42 nasal secretion samples collected. The volatile metabolite signature of SNIP plasma and nasal secretion samples were compared with those of healthy controls using chromatography.ResultsSeventy‐two volatiles were identified in plasma samples. Multivariate analysis of variance results, even when controlled for smoking status, indicated toluene as a significant univariate result with lower levels of toluene identified in SNIP plasma samples than healthy control plasma samples. A linear discriminant analysis (LDA) model for plasma volatiles correctly classified 23 of 24 SNIP patients and 26 of 27 control patients, with a cross‐validation error rate of 6.02%. Sixty‐nine volatiles were identified in nasal samples. For nasal secretion samples, no single univariate response was significant. The LDA model correctly classified 21 of 21 SNIP patients and 11 of 12 control patients, with a cross‐validation error rate of 6.55%.ConclusionThis study suggests that SNIP produces a unique, detectable volatile metabolite signature. With further investigation, this can have dramatic clinical implications for diagnosis and monitoring. Although most volatile metabolite studies have investigated solid‐organ malignancy, this novel study addresses a benign sinonasal neoplasm by using nasal secretions and plasma as an analysis medium, representing the first such study.
Occupational complexity and cognition in the FINGER multidomain intervention trial
Authors: Rydström Anders , Darin‐Mattsson Alexander , Kåreholt Ingemar , Ngandu Tiia , Lehtisalo Jenni , Solomon Alina , Antikainen Riitta , Bäckman Lars , Hänninen Tuomo , Laatikainen Tiina , Levälahti Esko , Lindström Jaana , Paajanen Teemu , Havulinna Satu , Peltonen Markku , Sindi Shireen , Soininen Hilkka , Neely Anna Stigsdotter , Strandberg Timo , Tuomilehto Jaakko , Kivipelto Miia , Mangialasche Francesca 
Published: February 2022

AbstractIntroductionLifetime exposure to occupational complexity is linked to late‐life cognition, and may affect benefits of preventive interventions.MethodsIn the 2‐year multidomain Finnish Geriatric Intervention Study to Prevent Cognitive Impairment and Disability (FINGER), we investigated, through post hoc analyses (N = 1026), the association of occupational complexity with cognition. Occupational complexity with data, people, and substantive complexity were classified through the Dictionary of Occupational Titles.ResultsHigher levels of occupational complexity were associated with better baseline cognition. Measures of occupational complexity had no association with intervention effects on cognition, except for occupational complexity with data, which was associated with the degree of intervention‐related gains for executive function.DiscussionIn older adults at increased risk for dementia, higher occupational complexity is associated with better cognition. The cognitive benefit of the FINGER intervention did not vary significantly among participants with different levels of occupational complexity. These exploratory findings require further testing in larger studies.
Progranulin mutations in clinical and neuropathological Alzheimer's disease
Authors: Vardarajan Badri N. , Reyes‐Dumeyer Dolly , Piriz Angel L. , Lantigua Rafael A. , Medrano Martin , Rivera Diones , Jiménez‐Velázquez Ivonne Z. , Martin Eden , Pericak‐Vance Margaret A. , Bush William , Farrer Lindsay , Haines Jonathan L. , Wang Li‐San , Leung Yuk Yee , Schellenberg Gerard , Kukull Walter , De Jager Philip , Bennett David A. , Schneider Julie A. ,   Alzheimer's Disease Sequencing Project, Mayeux Richard 
Published: February 2022

AbstractIntroductionProgranulin (GRN) mutations occur in frontotemporal lobar degeneration (FTLD) and in Alzheimer's disease (AD), often with TDP‐43 pathology.MethodsWe determined the frequency of rs5848 and rare, pathogenic GRN mutations in two autopsy and one family cohort. We compared Braak stage, β‐amyloid load, hyperphosphorylated tau (PHFtau) tangle density and TDP‐43 pathology in GRN carriers and non‐carriers.ResultsPathogenic GRN mutations were more frequent in all cohorts compared to the Genome Aggregation Database (gnomAD), but there was no evidence for association with AD. Pathogenic GRN carriers had significantly higher PHFtau tangle density adjusting for age, sex and APOE ε4 genotype. AD patients with rs5848 had higher frequencies of hippocampal sclerosis and TDP‐43 deposits. Twenty‐two rare, pathogenic GRN variants were observed in the family cohort.DiscussionGRN mutations in clinical and neuropathological AD increase the burden of tau‐related brain pathology but show no specific association with β‐amyloid load or AD.
Trans‐seeding of Alzheimer‐related tau protein by a yeast prion
Authors: Flach Martin , Leu Cedric , Martinisi Alfonso , Skachokova Zhiva , Frank Stephan , Tolnay Markus , Stahlberg Henning , Winkler David T. 
Published: February 2022

AbstractAbnormal tau protein aggregates constitute a hallmark of Alzheimer's disease. The mechanisms underlying the initiation of tau aggregation in sporadic neurodegeneration remain unclear. Here we investigate whether a non‐human prion can seed tau aggregation. Due to their structural similarity with tau aggregates, we chose Sup35NM yeast prion domain fibrils for explorative tau seedings. Upon in vitro incubation with tau monomers, Sup35NM fibrils promoted the formation of morphologically distinct tau fibril strains. In vivo, intrahippocampal inoculation of Sup35NM fibrils accentuated tau pathology in P301S tau transgenic mice. Thus, our results provide first in vivo evidence for heterotypic cross‐species seeding of a neurodegenerative human prion‐like protein by a yeast prion. This opens up the conceptual perspective that non‐mammalian prions present in the human microbiome could be involved in the initiation of protein misfolding in neurodegenerative disorders, a mechanism for which we propose the term “trans‐seeding.”
Air quality improvement and incident dementia: Effects of observed and hypothetical reductions in air pollutant using parametric g‐computation
Authors: Letellier Noémie , Gutierrez Laure‐Anne , Duchesne Jeanne , Chen Chen , Ilango Sindana , Helmer Catherine , Berr Claudine , Mortamais Marion , Benmarhnia Tarik 
Published: February 2022

AbstractIntroductionNo evidence exists about the impact of air pollution reduction on incidence of dementia. The aim of this study was to quantify how air quality improvement leads to dementia‐incidence benefits.MethodsIn the French Three‐City cohort (12 years of follow‐up), we used parametric g‐computation to quantify the expected number of prevented dementia cases under different hypothetical interventions with particulate matter measuring <2.5 μm (PM2.5) reductions.ResultsAmong 7051 participants, 789 participants developed dementia. The median PM2.5 reduction between 1990 and 2000 was 12.2 (μg/m3). Such a reduction reduced the risk of all‐cause dementia (hazard ratio [HR], 0.85; 95% confidence interval [CI], 0.76 to 0.95). If all study participants were enjoying a hypothetical reduction of more than 13.10 μg/m3 (median reduction observed in the city of Montpellier), the rate difference was −0.37 (95% CI, −0.57 to −0.17) and the rate ratio was 0.67 (95% CI, 0.50 to 0.84).DiscussionThese findings highlight the possible substantial benefits of reducing air pollution in the prevention of dementia.
The Electronic Origin of Far‐Red‐Light‐Driven Oxygenic Photosynthesis
Authors: Sirohiwal Abhishek , Pantazis Dimitrios A. 
Published: February 2022

AbstractPhotosystem‐II uses sunlight to trigger charge separation and catalyze water oxidation. Intrinsic properties of chlorophyll a pigments define a natural “red limit” of photosynthesis at ≈680 nm. Nevertheless, charge separation can be triggered with far‐red photons up to 800 nm, without altering the nature of light‐harvesting pigments. Here we identify the electronic origin of this remarkable phenomenon using quantum chemical and multiscale simulations on a native Photosystem‐II model. We find that the reaction center is preorganized for charge separation in the far‐red region by specific chlorophyll–pheophytin pairs, potentially bypassing the light‐harvesting apparatus. Charge transfer can occur along two distinct pathways with one and the same pheophytin acceptor (PheoD1). The identity of the donor chlorophyll (ChlD1 or PD1) is wavelength‐dependent and conformational dynamics broaden the sampling of the far‐red region by the two charge‐transfer states. The two pathways rationalize spectroscopic observations and underpin designed extensions of the photosynthetically active radiation limit.
Harnessing GLUT1‐Targeted Pro‐oxidant Ascorbate for Synergistic Phototherapeutics
Authors: Koo Seyoung , Lee Min‐Goo , Sharma Amit , Li Mingle , Zhang Xingcai , Pu Kanyi , Chi Sung‐Gil , Kim Jong Seung 
Published: February 2022

AbstractDespite extensive efforts to realize effective photodynamic therapy (PDT), there is still a lack of therapeutic approaches concisely structured to mitigate the major obstacles of PDT in clinical applications. Herein, we report a molecular strategy exploiting ascorbate chemistry to enhance the efficacy of PDT in cancer cells overexpressing glucose transporter 1 (GLUT1). AA‐EtNBS, a 5‐O‐substituted ascorbate–photosensitizer (PS) conjugate, undergoes a reversible structural conversion of the ascorbate moiety in the presence of reactive oxygen species (ROS) and glutathione (GSH), thereby promoting its uptake in GLUT1‐overexpressed KM12C colon cancer cells and perturbing tumor redox homeostasis, respectively. Due to the unique pro‐oxidant role of ascorbate in tumor environments, AA‐EtNBS effectively sensitized KM12C cancer cells prior to PS‐mediated generation of superoxide radicals under near‐infrared (NIR) illumination. AA‐EtNBS successfully exhibited GLUT1‐targeted synergistic therapeutic efficacy during PDT both in vitro and in vivo. Therefore, this study outlines a promising strategy employing ascorbate both as a targeting unit for GLUT1‐overexpressed cancer cells and redox homeostasis destruction agent, thereby enhancing therapeutic responses towards anticancer treatment when used in conjunction with conventional PDT.
Reversible Uptake of CO2 by Pincer Ligand Supported Dimetallynes
Authors: Caise Alexa , Griffin Liam P. , McManus Caitilín , Heilmann Andreas , Aldridge Simon 
Published: February 2022

AbstractWe report on the reversible uptake of carbon dioxide by dimetallynes featuring ancillary hemi‐labile pincer ligands. Insertion into the Ge−Ge/Sn−Sn bonds yields species containing an E(CO2)E unit, with the mode of ligation of the CO2 fragment determined crystallographically being found to be dependent on the identity of the Group 14 element. The thermodynamics of CO2 uptake/loss can be established through VT NMR (ΔH°=+24.6(2.3) kJ mol−1, ΔS°=+64.9(3.8) J mol−1 K−1, ΔG°298=+5.3(1.9) kJ mol−1 for the loss of CO2 in the Ge case), and the chemical consequences of reversibility demonstrated by thermodynamically‐controlled exchange reactions.
Single‐Atom Nanozymes for Biomedical Applications: Recent Advances and Challenges
Authors: Tang Minglu , Li Jingqi , Cai Xinda , Sun Tiedong , Chen Chunxia 
Published: February 2022

AbstractNanozymes have received extensive attention in the fields of sensing and detection, medical therapy, industry, and agriculture thanks to the combination of the catalytic properties of natural enzymes and the physicochemical properties of nanomaterials, coupled with superior stability and ease of preparation. Despite the promise of nanozymes, conventional nanozymes are constrained by their oversized size and low catalytic capacity in sophisticated practical application environments. single‐atom nanozymes (SAzymes) were characterized as nanozymes with high catalytic efficiency by uniformly distributed single atoms as catalysis sites, thus effectively addressing the defects of conventional nanozymes. This paper reviews the activity improvement scheme and catalytic mechanism of SAzymes and highlights the latest research progress of SAzymes in the fields of biomedical sensing and therapy. Eventually, the challenges and future directions of SAzymes are discussed in this paper.
Approved spectrofluorimetric strategies for assurance of three modern antineoplastic drugs: tepotinib, sotorasib, and darolutamide in their dose forms and biological liquids utilizing mercurochrome
Authors: Salem Hesham , Abdal‐Karim Noha , Omran Bahaa , Abdel‐Gayed Abdel‐Rahman , Atef Mazen , Abdelgaleel Mahmoud 
Published: February 2022

AbstractAn approved, straightforward, fast, and delicate spectrofluorimetric strategy was developed for the estimation of tepotinib (TEPO), sotorasib (SOTO), and darolutamide (DARO) as new antineoplastic drugs. The spectrofluorimetric strategy was based on quantitative fluorescence quenching of MER at 538 nm after being excited at 350 nm by the addition of the cited drugs in the presence of acetate buffer (pH 3.5). The degree of fluorescence quenching was directly proportional to the concentrations of the cited drugs within the concentration range of 0.5–10.0, 0.2–10, and 0.4–10.0 μg ml−1for TEPO, SOTO, and DARO, respectively. Mean ± standard deviation (SD) were calculated for the studied drugs as follows; 99.9 ± 0.87, 99.72 ± 1.08, and 100.21 ± 1.44, for TEPO, SOTO, and DARO, respectively. Limit of detection (LOD) values were 0.16, 0.05, and 0.11 μg ml−1, whereas limit of quantitation (LOQ) values were 0.5, 0.15, and 0.36 μg ml−1for TEPO, SOTO, and DARO, respectively. Statistical comparison through detailed strategies produced greater understanding and found that there were no noteworthy contrasts in exactness and exactness between strategies. The proposed strategy was used effectively to analyze the measurement of different forms of the examined drugs. Moreover, the recommended fluorimetric strategy was used for examination of TEPO, SOTO, and DARO in human plasma and urine tests.
Age‐specific effectiveness of primary human papillomavirus screening versus cytology in a cervical cancer screening program: a nationwide cross‐sectional study
Authors: Bao Heling , Ma Lan , Zhao Yanxia , Song Bo , Di Jiangli , Wang Linhong , Gao Yanqiu , Ren Wenhui , Wang Shi , Wu Jiuling , Wang Hai‐Jun 
Published: February 2022

AbstractBackgroundPrimary human papillomavirus (HPV) screening is recommended for the detection of cervical intraepithelial neoplasia (CIN) in the general population; however, the triage for HPV‐positive women remains a challenge. This study aimed to evaluate the age‐specific effectiveness of primary HPV screening versus primary cytology screening for identifying optimal strategies for women of different ages.MethodsThe dataset of the prevalence round screening was derived from the National Cervical Cancer Screening Program in China. Primary cervical screening protocols included cytology only, HPV testing with cytology triage, and HPV testing with HPV‐16/18 genotyping plus cytology triage. The primary outcomes were age‐specific detection rate, colposcopy referral rate and positive predictive value (PPV) for CIN2+. Multivariate Poisson regression was used to evaluate the relative effectiveness of HPV testing and cytology according to age groups. The I2 statistic with a random‐effect model was used to test the heterogeneity in relative effectiveness of HPV testing versus cytology between age groups.ResultsThis study included 1,160,981 women. HPV testing with HPV‐16/18 genotyping plus cytology triage significantly increased the CIN2+ detection by 36% (rate ratio [RR]: 1.36, 95% confidential interval [CI] 1.21–1.54) for women aged 35‐44 years and by 34% (RR: 1.34, 95% CI 1.20‐1.51) for women aged 45‐54 years compared with cytology only. HPV testing with cytology triage had similar CIN2+ detection rate compared with cytology only. The PPVs were substantially increased for both HPV testing groups. Among women aged 55‐64 years old, HPV testing with HPV‐16/18 genotyping plus cytology triage increased the colposcopy referral rate by 19% (RR 1.19, 95% CI 1.10‐1.29) compared with cytology only, but did not increase the CIN2+ detection (1.09, 0.91–1.30). The effectiveness of HPV testing with cytology triage did not change in older women. The between‐age‐group heterogeneity in the effectiveness was statistically significant for HPV testing with HPV‐16/18 genotyping plus cytology triage versus cytology only.ConclusionsOur results suggested that the effectiveness of primary HPV screening with different triage strategies differed among age groups. HPV testing with HPV‐16/18 genotyping plus cytology triage could be used for women aged 35‐54 years to detect more lesions, and HPV testing with cytology triage could balance the CIN2+ detection and the number of colposcopies for women aged 55‐64 years. Longitudinal data including both prevalence and incidence screening rounds are warranted to assess age‐specific triage strategies.
Open‐source terrestrial laser scanner for the virtualization of geometrical entities in AEC classrooms
Authors: Ramonell Carlos , Chacón Rolando 
Published: February 2022

AbstractThis paper depicts a case study that shows an open‐source Terrestrial Laser Scanner (TLS) for use on the virtualization of simple yet precise geometrical entities in AEC classrooms. For bringing this technology to AEC classrooms, an open‐source TLS was developed. The physical model was built using digital fabrication, its connectivity was established using accessible, affordable, open‐source sensors and actuators and its digital representation was developed using generative design tools. All elements together are synchronized in real‐time and the TLS becomes live, digitally twinned, geometry generator. The points that are generated by the TLS are gathered in a 3D virtual space in the form of virtual points. The present digitally twinned vision of laser scanning has thus three identified educational uses: (i) illustration of the measuring principle using open‐source hardware together with mathematics and statistics, (ii) illustration of the generation and visualization of point clouds in real‐time within a CAD environment, and (iii) perhaps with a vaster scope in AEC classrooms, illustration of the usage, analysis and identification of these point clouds. The development of this tool belongs to a vaster project for infusing Construction 4.0 technologies in AEC classrooms under development at the School of Civil Engineering of UPC‐BarcelonaTech. The illustration to students of all these concepts with accessible technological tools is expected to enlarge their vision of more advanced constructional technologies such as cyber‐physical systems for monitoring and surveying as well as digital technologies for reproducing “As‐Built” models.