bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Analysis of a Cuproptosis-Related Gene Index in Pancreatic Adenocarcinoma with Significant Implications on Prognosis and Immunotherapy as well as Chemotherapy Efficacy
Authors: Huang Xufeng , Zhou Shujing , Liu Ying , Tóth János , András Hajdu 
Published: June 2022

Abstract

Background
A novel type of cell death induced by intracellular copper accumulation, principally different from other known ones such as autophagy, ferroptosis, and pyroptosis, was discovered and referred to as “Cuproptosis” recently. As the underlying mechanism was newly identified, its potential connection to pancreatic adenocarcinoma (PAAD) is still an open issue. However, interestingly, one of the 10 Cuproptosis-related genes, CDKN2A, was previously reported as a driver mutation gene in pancreatic adenocarcinoma. Therefore, it raised our interest to develop a Cuproptosis-related gene index (CRGI) for better overall survival (OS) estimation, explore the corresponding relationship with tumor immunology, and discuss the chemotherapy efficacy of commonly used anti-cancerous drugs relevant to CRGI.
Methods
Gene expression profiles and matched clinical data were curated from publicly accessible sources from which the CRGI was developed. Immunogenic properties were investigated according to the immune checkpoints, potential response to the immunotherapy, cancer immunity cycle, etc. The sensitivity to chemotherapy agents was evaluated through the IC50 curve. The consensus clustering approach was implemented to robustly identify the CRGI-based molecular subtypes and explore the immunotherapy and chemotherapy efficacies.
Results
A novel gene signature derived from Cuproptosis was established. Compared with those signatures originating from other cell death mechanisms, it exerted a more robust yet more accurate predictive performance. Besides, the result of correlation analysis unraveled the distinct differences in tumor immunology between the high- and low-risk groups. Through in-depth analysis of the IC50 curve of each drug, the predictive chemotherapy efficacy of 32 commonly used anti-cancerous drugs was also found various in high- and low-risk groups. The analysis of molecular subtypes has further confirmed these ideas.
Conclusion
In the present study, we highlighted the outstanding achievement of CRGI in PAAD prognostic prediction and its association with tumor immunology. These findings may benefit future immunotherapy- and chemotherapy-based interventions.
Brucine-induced neurotoxicity by targeting caspase 3: Involvement of PPARγ/NF- κB/apoptosis signaling pathway
Authors: Lei Yaying , Hou Fangqin , Wu Xiaoyu , Yi Yang , Xu Fan , Gong Qihai , Gao Jianmei 
Published: June 2022

Abstract
Brucine, a weak alkaline indole alkaloid, is one of the main bioactive and toxic constituents of Strychnos nux-vomica L., which exerts multiple pharmacological activities, such as anti-tumor, anti-inflammatory, analgesic effect. However, its potential toxic effects limited its clinical application, especially central nervous system toxicity. The present study was designed to investigate the neurotoxicity and mechanism of brucine. Our results showed that brucine significantly induced Neuro-2a cells and primary astrocytes death, as evidenced by MTT assay and LDH release. Moreover, transcriptome analysis indicated that PPAR/NF-κB and apoptosis signaling pathways were involved in the brucine-induced cytotoxicity in Neuro-2a cells. Subsequently, in fact, brucine evidently inhibited PPARγ, promoted phosphorylation of NF-κB. Furthermore, PPARγ inhibitor aggravated the neurotoxicity, while, NF-κB inhibitor substantially reversed brucine-induced neurotoxicity. Moreover, brucine also significantly induced neuronal apoptosis and triggered increase in ratio of Bax/Bcl-2, level of cleaved caspase-3, as well as its activity as evidenced by TUNEL staining and Western blot. Furthermore, molecular docking analysis predicted that brucine directly bound to caspase 3. Intriguingly, a caspase 3 inhibitor (Z-DEVE-FMK) largely abolished the neurotoxicity of brucine. Our results reveal that brucine-induced neurotoxicity via activation of PPARγ/NF-κB/caspase 3-dependent apoptosis pathway. These findings will provide a novel strategy against brucine-induced neurotoxicity.
Dynamic transcriptome profiling of salt-responsive long noncoding RNAs in quinoa
Authors: Luo Chuping , He Bing , Shi Pibiao , Pang Bingwen , Cheng Junjie , Hu Fengqin , Qi Weicong , Lv Yuanda 
Published: June 2022

Abstract
Background Chenopodium quinoa is a crop with outstanding tolerance to saline soil, but long noncoding RNAs (LncRNAs) expression profile driven by salt stress in quinoa has rarely been observed yet. Based on the high-quality quinoa reference genome and high-throughput RNA sequencing (RNA-seq), genome-wide identification of LncRNAs was performed, and their dynamic response under salt stress was then investigated. Results In total, 153,751 high-confidence LncRNAs were discovered and dispersed intensively in chromosomes. Expression profile analysis demonstrated significant differences between LncRNAs and coding RNAs. Under salt stress conditions, 4,460 differentially expressed LncRNAs were discovered, of which only 54 were differentially expressed at all the stress time points. Furthermore, a weighted co-expression network was then constructed to infer the potential biological functions of LncRNAs. Seven modules were significantly correlated with salt treatments, resulting in 210 hub genes, including 22 transcription factors and 70 LncRNAs. These results indicated that LncRNAs might interact with transcription factors to respond to salinity stress. Gene ontology enrichment of the coding genes of these modules showed that they were highly related to regulating metabolic processes, biological regulation and response to stress. Conclusions This study is the first report on genome-wide analysis of the LncRNAs responding to salt stress in quinoa. The findings will provide a solid framework for further functional research of salt responsive LncRNAs, contributing to quinoa genetic improvement.
Diabetes-Related Complications and Mortality in Patients with Atrial Fibrillation Receiving Different Oral Anticoagulants
Authors: Shakeel Reem , Munir Sheheryar , Mazhar Naqash 
Published: June 2022

Abstract
Introduction: Globally, cardiovascular disease is the main contributor to mortality, accounting for about 17.5 million deaths, or 46.2% of deaths from non-communicable diseases. Objectives: The main objective of the study is to find the diabetes-related complications and mortality in patients with atrial fibrillation receiving different oral anticoagulants. Material and methods: This cross-sectional study was conducted in Services Institute of medical sciences, Lahore during June 2021 to December 2021. The data were collected from 120 diabetic patients who diagnosed with AF. After permission from hospital ethical committee, total 120 patients meeting the inclusion and exclusion criteria was enrolled in the study. Detailed history of DM and physical examination was done to meet the inclusion and exclusion criteria. Results: The data was collected from 120 patients. Out of 120 participants, 60 were treated with warfarin while 60 were considered as control group. Median age was 26 years in the group I and 25.3 years in the group II (p=0.705). Female cases counted for 41 (86%) and 19 (14%) in I and II groups, respectively. Conclusion: It is concluded that patients with AF and diabetes have a high overall cardiovascular risk. Non–vitamin K antagonist oral anticoagulants were associated with lower hazards of diabetes complications and mortality than warfarin in patients with AF and DM.
Sudanese Communications GEO Satellite: A case study on SUDASAT
Authors: Ahmed Mayada A M , Saeed Rashid A 
Published: June 2022

Abstract
recently, and to cater to increased needs for communicating through satellite, there has been a growing interest in the marketplace as well as in the research community to develop advanced applications utilizing satellite communications systems. Satellite communications are becoming a common trend in Arab countries. A growing body of literature has focused on developing Geostationary Satellite (GEO) satellites. However, International Telecommunications Union (ITU) have the main coordination for all GEO satellite around the world. The present paper seeks to address the main timelines which relate to a Sudanese satellite. This research study is focused on GEO SUDASAT to offer a variety of communications applications in Sudan. The obtained data is provided from Space Capture ITU software. In this paper, we investigate a proposal for a Sudanese satellite called SUDASAT. The main goal of this paper is to define the SUDASAT technical information which consists of satellite orbital position, the main earth station, and its look angles, the coverage area, launch vehicle, satellite manufacturer and the suitable platform, EIRP, G/T, frequency bands, and several transponders.
The influence of mixing technology on the uniformity and strength of cement stabilized macadam mixture
Authors: Wang Jianhong , Wang Jinyong , Wang Quanlei , Zhang Dongchang , Du Bin , Wang Huoming , Liang Xuzhi 
Published: June 2022

A comparison between the two strategies: secondary traditional mixing technology and secondary vibratory mixing technology is proposed based on aggregate sieving, cement EDTA titration and unconfined compressive strength tests to explore the influence of the uniformity and strength of cement stabilized macadam mixture in the paper. The results show that when the secondary vibratory mixing technology is adopted: (1) the standard deviations and coefficients of variation of the passing rates of key sieves of 19 mm, 13.2 mm, 9.5 mm, 4.75 mm and the cement dosage titration results decrease significantly, and the mixing uniformity of the mixture is significantly improved; (2) the 7-day and 28-day unconfined compressive strengths of the mixtures are significantly improved while the strength standard deviations and coefficients of variation are reduced, with an obvious trend along with the extension of the curing time (within 28 days).
Therapeutic Implications of Curcumin in the Treatment of Inflammatory
Eye Diseases: A Review
Authors: Thiruvengadam Muthu , Heydari Mojtaba , Khalili Mohammad Reza , Nowroozzadeh Mohammad Hossein , Bamdad Shahram , Sahebkar Amirhossein , Shariati Mohammad Ali 
Published: June 2022

Abstract:
Curcumin is the main active constituent of the medicinal plant Curcuma longa L., used
traditionally as a medicinal spice in several ancient civilizations. Different preclinical and clinical
studies support the anti-inflammatory properties of curcumin in various inflammatory diseases. As
inflammation has an essential role in the pathophysiology of many ocular diseases, curcumin has
been suggested as a promising therapeutic agent with anti-inflammatory properties. Based on the
extent of experimental and clinical evidence, curcumin can exert protective effects against the
corneal, uveal, retinal, optic nerve, orbital, and lacrimal gland inflammatory disorders. Herein, the
available literature on the beneficial effects of curcumin in inflammatory eye diseases is reviewed.
The limitations and future directions of these investigations are also discussed.
Anticancer Drug Development: Evaluative Architecture
Authors: Lu Da-Yong , Xu Bin , Lu Ting-Ren 
Published: June 2022

Abstract:
Anticancer drug development is complex and multi-factorial, demanding robust drug evaluative
architecture in experimental and preclinical studies. To increase the number of drug licensing, biochemical,
pharmacological, technical and economic changes (balance and integration) in evaluative systems
should be focused in labs throughout the world. Despite great progress, treatment breakthroughs and
drug industry need new ideas and more competitive technology (new generation of in vitro test systems).
Overall, drug evaluative systems comprise anticancer drug development (medicinal chemistry and pharmacology)
from initial screening to clinical validity. Its advances greatly impact the outcomes of drug
production (rates of final drug licensing and efficacy of therapeutics in the clinic). In this regard, creative
ideas and new techniques will change the norm and convention of drug screening and mechanic exploration
in the future. This article provides multi-disciplinary approaches for experimental and preclinical
anticancer drug evaluations, selections and combinations (chemistry and biomedicine). Future trends for
drug evaluative systems are especially highlighted in in-depth, multilateral and multidisciplinary approaches.
Age Related Osteoarthritis: Regenerative Therapy, Synthetic Drugs, and
Naturopathy to Combat Abnormal Signal Transduction
Authors: Mukherjee Jhumpa , Samadder Asmita , Nandi Sisir , Ahmed Tamanna , Dey Rishita 
Published: June 2022

Introduction:
Osteoarthritis (OA) is a common chronic inflammatory neurodegenerative joint disorder that causes disability among the geriatric population. It involves the loss of the articular cartilage that covers the end of a long bone thereby failing to prevent the friction between the joints.

Method:
The literature on the prevalence of OA and different risk factors like physical inactivity, obesity, and joint injury was searched through Google scholar, PubMed, research gate, Wikipedia, etc for the review.

Result:
OA has affected around 303 million people globally. It affects the knee, hip, hands, and spine joints owing to common symptoms like pain, swelling, and disability. Further, OA-associated disability causes depression leading to an economic and social burden with physical isolation, thus making it more severe for older people in their day-to-day lifestyle. Presently, no permanent cure has been devel-oped for OA. Although, there are many risk factors of OA, among them, the most prominent one is considered to be “aging”. Most people crossing the age of 65–70 years have been associated with changes in the joints (one or more) about the development of OA. Several theories related to cellular aging and cell senescence with OA development. However, aging alone does not cause this condition; it is accelerated by the abnormal signal transduction followed by the progression of OA. The blueprint of possible management of OA by the different approaches has been the prime concern of this review work.

Conclusion:
An outline of the risk factors of abnormal signal transduction and different treatment ap-proaches, including regenerative therapy, synthetic drugs, and naturopathy manipulating them concern-ing OA are discussed in this review which might be an answer to the age-old issue of geriatrics.
Design, Synthesis, Docking Studies, Enzyme Inhibitory and Antiplatelet Aggregation Activities of New 1,3-Diphenyl-3-(Phenylthio)Propan-1-One Derivatives as Selective COX-2 Inhibitors
Authors: Daraei Bahram , Zarghi Afshin , Bayanati Maryam 
Published: June 2022

Background:Cancer is the second leading cause of death worldwide after heart disease. A vast number of studies indicated that selective cyclooxygenase-2 (COX-2) inhibitors could be chemopreventive against different types of cancer because the expression of COX-2 is increased. Therefore, to develop new therapeutics for cancer, the design and synthesis of new COX-2 inhibitors with few side effects seem attractive as anti-cancer agents.Objective:Some of the well-known drugs that have been widely used for some time have been removed from the market due to the cardiac side effects they cause, so there is a need to introduce a scaffold that can inhibit COX-2 with high potency and low side effects. This study aimed to introduce a new COX-2 inhibitor structure.Methods:A new series of β-aryl-β-mercapto ketones possessing a methylsulfonyl pharmacophore was synthesized and evaluated as selective COX-2 inhibitors. In-vitro COX-1 and COX-2 inhibition effects of these compounds were evaluated, and molecular modeling was examined. Also, the antiplatelet aggregation activity of the synthesized compounds was tested.Results:In-vitro COX-1 and COX-2 inhibition assays indicated that almost all newly synthesized compounds showed selectivity for COX-2 with IC50 values in the 0.07-0.22 μM range and COX-2 selectivity indexes in the 170 to 703.7 range. Among the tested compounds 1-(4-(methylsulfonyl)phenyl)-3-phenyl-3-(phenylthio)propan-1-one (4a), 3-(3,4- dimethoxyphenyl)-1-(4-(methylsulfonyl)phenyl)-3-(phenylthio)propan-1-one (4g) and 3-(4-fluorophenyl)-1-(4-(methyl sulfonyl)phenyl)-3-(phenylthio)propan-1-one (4h) were the most potent COX-2 inhibitors and 3-(3,4- dimethoxyphenyl)-1-(4-(methylsulfonyl)phenyl)-3-(phenylthio)propan-1-one had the highest selectivity index for COX-2 enzyme inhibitory activity. The Anti-platelet aggregation activity results indicated that the compound 1-(4- (methylsulfonyl)phenyl)-3-(phenylthio)-3-(p-tolyl)propan-1-one (4b) possesses the strong anti-platelet activity. Our molecular modeling studies also indicated that the methylsulfonyl pharmacophore group is placed into the adjunct pocket in the COX-2 active site and forms hydrogen bond interactions with NH of Arg513 and NH of His90.Conclusion:In brief, all designed and synthesized compounds showed moderate to good COX-2 inhibitory effects and showed good anti-platelet activity. Therefore, these compounds have the potential for further research into developing anti-cancer agents.
Postural Orthostatic Tachycardia Syndrome and Orthostatic Hypotension
Post COVID-19
Authors: Shahmohamadi Elnaz , Eslami Masoud , Mollazadeh Reza , Mirshafiee Shayan , Sehat Parisa , Alizadeh Fatemeh , Emkanjoo Zahra , Far Vahideh Laleh 
Published: June 2022

Background:
Novel coronavirus causes coronavirus disease -19 (COVID-19). The hallmark
is acute respiratory distress syndrome, but other systems' involvement is less illustrated. Our goal was
to evaluate the manifestation of COVID-19 on one of the overlaps of the cardiovascular and nervous
system, namely: Postural Orthostatic Tachycardia Syndrome (POTS) and Orthostatic Hypotension
(OH).

Methods:
This single-center cross-sectional observational study encompassed 60 consecutive patients
that were hospitalized and recovered from severe or critical COVID-19. At the time of discharge,
Blood Pressure (BP) and Heart Rate (HR) in the supine and upright position (1st, 3rd, 5th and 10th
minutes) were measured. Symptomatic patients were reevaluated 2 months later.

Results:
The mean age of patients was 56.6 (± 16.2) years and 42 patients were male (70%). The most
frequent cardiovascular risk factor was hypertension (35%). OH and POTS were detected in 29(48.3%)
and 10(16.7%) patients, respectively, at the time of hospital discharge. The mean age of patients with
OH was higher than POTS and POTS was frequent in the elderly. Two months later, among 10 patients
with POTS, the sign and symptoms were resolved in 8(80%). Two (20%) patients who still had positive
signs and symptoms of POTS were older than 65 years. Among 29 patients with OH, the signs and
symptoms were resolved in 26 (89.7%).

Conclusion:
In our study, 65% of patients had OH or POTs on the day of hospital discharge; complete
recovery is gradual and needs several additional weeks. This is one of the aspects of the entity recently
named “Long COVID”.
Cellulose Nanomaterials Based Flexible Electrodes for All-Solid-State Supercapacitors
Authors: Gao Mengge , Du Haishun 
Published: June 2022

Abstract:
In recent years, flexible all-solid-state supercapacitors have been widely used as the energy storage devices for various smart and wearable electronic devices. However, the design and fabrication of high-performance flexible supercapacitor electrodes is still challenging since most of the active materials used for supercapacitor electrodes lack the ability to form flexible and mechanically stable structures. Recently, cellulose nanomaterials (mainly include cellulose nanocrystals and cellulose nanofibrils) have gained extensive interests due to their large specific surface areas, versatile surface chemistry, high mechanical strength, and the ability to form mechanically stable structures (e.g., films, aerogels). These days, the design of flexible supercapacitor electrodes by combining cellulose nanomaterials with different active materials gradually attracted the attention of scholars. The main objective of this review is to give an overview of recent developments in the preparation of cellulose nanomaterials based flexible all-solid-state supercapacitor electrodes. The fabrication approach, structure characterization, and electrochemical performance of the invented cellulose nanomaterials based flexible supercapacitor are elaborated. Also, the current challenges and future outlook for the design and fabrication of cellulose nanomaterials based flexible all-solid-state supercapacitor are proposed.
Fats of Pigs of Different Breeds and Chemical Composition in the Diet of
Animals
Authors: Bekenev Vitaly A. , Mager Sergey N. , Arishin Anatoly A. , Merzlyakova Olga G. , Kashtanova Elena V. , Polonskaya Yana V. , Chegodaev Victor G. , Frolova Valentina I. , Bolshakova Izolda V. , Frolova Yulia V. , Ites Yuri V. 
Published: June 2022

Background:
The studies were carried out on quails, in the feed of which fat of various
compositions was added.

Objective:
To establish the effect of adding fat from pigs of different breeds and vegetable oil to quail
feed on their growth, productivity, and characteristics of lipid metabolism.

Methods:
Three groups of 60 chickens each were fed with vegetable oil (control) and fat of two pig
breeds sharply differing in the chemical composition of fat - Kemerovo (К) and Pietrain (P) up to 23
weeks of age (3.0 - 3.5% of the mass of the diet). At 8 and 23 weeks of age, 10 individuals from each
group underwent biochemical blood tests.

Results:
The most rapid growth was observed in quails fed with K-breed fat. The level of total cholesterol
(TC) during the period of the experiment in quails significantly decreased, including those receiving vegetable
oil - by 1.49 times, fat of the K breed - by 1.58 times, fat breed P - 1.32 times. The atherogenic index (AI)
turned out to be significantly higher (Р<0.01) in quails that received fat of the P breed, containing a high level
of TС and rich in PUFA (0.93 conventional units against 0.69 in birds that received fat of the K breed and
0.57 - in the control group).

Conclusion:
The obtained data indicate that fat of breed K is more useful for animal nutrition and
maybe a priority in human nutrition.
Versatile Applications of Nanotechnology-based Electronic Nose
Authors: Girigoswami Koyeli , Shwetha M  , Girigoswami Agnishwar , Deepika B  , Gopikrishna A  
Published: June 2022

Background:
Electronic noses have recently developed as a part of nanotechnology-based devices to cater to different needs in the field of biomedical sciences.

Objective:
To have a detailed idea regarding the various uses of electronic noses in the early detection of diseases, food spoilage, etc., we need to explore multiple studies.

Methods:
Chiefly, biosensors for detecting different volatile organic compounds responsible for different diseases exerted from the lungs are developed based on electronic noses. The difference in the breath pattern of normal individuals and diseased individuals who have cancer, CVD, diabetes, neurological disorders, etc. can be differentiated using the electronic nose.

Results:
In the field of food industry, bacterial contamination and other kinds of food spoilage can be detected at an early stage by detecting the pattern of evolved gases from these food items using the electronic nose.

Conclusion:
Thus, there are versatile applications of electronic noses in the early diagnosis of life-threatening diseases, chemical industry, food industries, etc., which will be discussed in this review.