bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between estimated glucose disposal rate and in-stent restenosis following percutaneous coronary intervention in individuals with non-ST-segment elevation acute coronary syndrome
Authors: Liu Chi , Zhao Qi , Ma Xiaoteng , Cheng Yujing , Sun Yan , Zhang Dai , Liu Xiaoli , Zhou Yujie 
Published: June 2022

Abstract
Background
Insulin resistance (IR) is closely associated with in-stent restenosis (ISR) following percutaneous coronary intervention (PCI). Nevertheless, the predictive power of the newly developed simple assessment of IR, estimated glucose disposal rate (eGDR), for ISR after PCI in individuals with non-ST-segment elevation acute coronary syndrome (NSTE-ACS) remains unclear.
Methods
Based on eligibility criteria, 1218 NSTE-ACS cases administered PCI in Beijing Anzhen Hospital Between January and December 2015 were included. The included individuals were submitted to at least one coronary angiography within 48 months after discharge. Patients were assigned to 2 groups according to ISR occurrence or absence. eGDR was derived as 21.16 - (0.09 * waist circumference [cm]) - (3.41 * hypertension) - (0.55 * glycated hemoglobin [%]). Multivariate logistic regression analysis model and receiver operating characteristic (ROC) curve analysis was established for evaluating eGDR’s association with ISR.
Results
In multivariate logistic analysis, the odds ratios (ORs) of eGDR as a nominal variate and a continuous variate were 3.393 (confidence interval [CI] 2.099–5.488, P < 0.001) and 1.210 (CI 1.063–1.378, P = 0.004), respectively. The incremental effect of eGDR on ISR prediction based on traditional cardiovascular risk factors was reflected by ROC curve analysis (AUC: baseline model + eGDR 0.644 vs. baseline model 0.609, P for comparison = 0.013), continuous net reclassification improvement (continuous-NRI) of -0.264 (p < 0.001) and integrated discrimination improvement (IDI) of 0.071 (p = 0.065).
Conclusion
In NSTE-ACS cases administered PCI, eGDR levels are independently negatively associated with increased ISR risk.
An enhancer demethylator phenotype tightly associated with immune exhaustion and resistance to immune checkpoint inhibitors in clear-cell renal cell carcinomas
Authors: Malouf Gabriel , Lu Xiaofan , Vano Yann , Helleux Alexandra , Su Xiaoping , Lindner Véronique , Davidson Guillaume , Mouawad Roger , Spano Jean-Philippe , Rouprêt Morgan , Elaidi Reza , Compérat Eva , Verkarre Virginie , Sun Chengming , Chevreau Christine , Bennamoun Mostefa , Lang Hervé , Tricard Thibault , Cheng Wenxuan , Xu Li , Davidson Irwin , Yan Fangrong , FRIDMAN Wolf , Sautès-Fridman Catherine , Oudard Stéphane 
Published: June 2022

Abstract
Immune checkpoint inhibitors have revolutionized the treatment of patients with clear-cell renal carcinomas. Although, analyses of transcriptome, genetic alterations, and the tumor microenvironment have shed light into mechanisms of response and resistance to these agents, the role of epigenetic alterations in this process remains fully unknown. Herein, we investigated the methylome of six clear-cell renal cell carcinomas cohorts and discovered a group of tumors with enhancer demethylation, namely \(TED\). \(TED\) was associated with tumors with sarcomatoid differentiation and poor clinical outcome. \(TED\) harbored TET1 and JAK3 promoter demethylation, activated the gene expression signature of epithelial-mesenchymal transition and IL-6/JAK/STAT3 pathways, and displayed a tumor microenvironment characterized by immune exhaustion, fibroblasts infiltration, and endothelial depletion. In addition, \(TED\) was the only predictive factor of resistance to the combination of first-line ipilimumab-nivolumab in the BIONIKK clinical trial. Finally, \(TED\) was associated with activation of specific regulons, which we also found to be predictive of resistance to immunotherapy in an independent cohort. In conclusion, we report on the discovery of a novel epigenetic phenotype associated with resistance to immune checkpoint inhibitors that may pave the way to better personalizing patients’ treatments.
One-Stage Transanal Endorectal Pull-through for Hirschsprung Disease: experience with 229 neonates
Authors: Zhang Yanan , Liu Zhaozhou , Li Shuangshuang , Yang Shen , Zhao Jiawei , Yang Ting , Li Siqi , Chen Yongwei , Guo Weihong , Hou Dawei , Li Yingzi , Huang Jinshi 
Published: June 2022

Abstract
Objective
To evaluate the safety and efficacy of the transanal endorectal pull-through (TEPT) and the long-term outcomes in newborns with HD.
Methods
A total of 229 newborns with HD underwent one-stage TEPT between 2007 and 2020, and the diagnoses were confirmed by rectal biopsy. The perioperative clinical course for all patients was reviewed, and the postoperative short- and long-term outcomes were assessed.
Results
A total of 229 neonates (187 male and 42 female) had a median age at TEPT of 17 days (range, 6–28 days). Sixty-eight patients (29.7%) underwent TEPT combined with an abdominal approach or laparoscopy. Early postoperative complications were documented in 26 patients (11.4%). The follow-up period in the remaining 165 children ranged from 1.2 to 14.0 years (median, 5.0 years), and late postoperative complications were noted in 6 patients (3.6%). A total of 106 of the patients older than four years old took part in an interview about bowel function, and 85 patients (80.2%) had a bowel function scores (BFS) ≥ 18.
Conclusion
TEPT is effective and safe in the treatment of neonatal HD and presents with a low rate of complications and an acceptable outcome.
A green synthetic method for co(ii,iii) oxide nanoparticles with high surface activity.
Authors: Absalan Yahya , Alabada Rusul , Razavi Mohammad Reza , Gholizadeh Mostafa , Kovalchukovac Olga 
Published: June 2022

Abstract
An environmentally friendly method is proposed to isolate Co3O4 nanoparticles with high surface activity. Instead of usual organic solvents, three different natural sugars, including glycogen, sucrose, and glucose are used for the first time as templates, and water as a green solvent, is used in all the steps. The crystallite sizes of the samples are 22.61 nm, 36.22 nm, and 30.093 nm for glucose, glycogen, and sucrose templates, respectively. The adsorption-desorption isotherms for all the samples match with IV type, which show a strong interaction between adsorbent and adsorbed sample. The pores of compounds obtained from glucose and glycogen templates, with H4 hysteresis, are slits and conical in the case of sucrose. The pore diameters are found in the interval 1.21–1.64 nm. The result illustrates a simple method with high novelty which can produce a variety of nanoparticles with a very large surface activity that can be used in different industries.
Scoping review using PRISMA-SCR on m-health interventions on self-care management of type 2 diabetes at home
Authors: Sinha Abhijeet Prasad , Singhal Manmohan , Gupta Mansi , Joshi Ashish 
Published: June 2022

Abstract
Objectives: To do scoping review on the global evidence on m-health interventions and determine the effectiveness of mobile based self-management interventions on type 2 diabetes mellitus Methods: A systematic search of PubMed and Scopus research data base were examined. Studies globally published in English from 2010 to 2020 and studies done on Type 2 diabetes and including age groups 18-75 years are included. The studies included intervention such as Randomized controlled trials (RCTs) of mobile based self-management for type 2 diabetes patients and charting and analysis of outcomes at patient level. The reviewer screened the studies, and it was consequently reviewed by a second reviewer independently. The data was extracted, charted and quality of the studies was assessed. The scoping study methodology using operational framework of Arksey, and O’Malley is used to present study outcomes and 27 pointers PRISMA-SCR checklist is used to present this paperResults: A total of 40 articles, consisting of RCT based interventions studies with 3800 plus participants, were included in the assessment. 40 original research articles were selected for inclusion. The selected studies largely focused on the self-management of type 2 diabetes (N = 40), hypertension (N = 2). Various interventions involved the use of mobile SMS (N = 17) or mobile on phone (N = 12), the content on session education with use of mobile (N = 10) or the placement of devices mobile (N = 4). The interventions were generally effective and often led to improved glycemic control and better health indicators.Conclusions: Mobile based self-management interventions seem to have positive effects on self-care and management and significant outcomes for patients with type 2 diabetes. Overall progress in physiologic indicators, selfcare and improvement in quality of patient life with Type 2 Diabetes. Though, the studies emphasized results in small populations, with little indication of future scaling of the intervention. Moreover, the results indicate a need for further research into the self-management which includes nutrition informatics in self-care management regime, and it needs to focus on region wise availability of informatics on nutrition. This will also help in co-management of diabetes and cardiovascular disease. Self-management appears to be an effective means of improving health outcomes in low- and middle-income countries (LMIC) and therefore mobile led selfcare management interventions with nutritional informatics can be advantageous.
Core genome sequencing and genotyping of Leptospira interrogans in clinical samples by target capture sequencing
Authors: Grillova Linda , Cokelaer Thomas , Mariet Jean-François , Fonseca Juliana Pipoli , Picardeau Mathieu 
Published: June 2022

Abstract
Background
The life-threatening pathogen Leptospira interrogans is the most common agent of leptospirosis, an emerging zoonotic disease. However, little is known about the strains that are circulating worldwide due to the fastidious nature of the bacteria and its difficulty to be culture isolated. In addition, the paucity of bacteria in blood and other clinical samples has proven to be a considerable challenge for directly genotyping the agent of leptospirosis directly from patient material.
Methods
Here, to elucidate the genomic diversity of Leptospira circulating strains, hybridization capture followed by Illumina sequencing of the core genome was performed directly from 20 clinicall samples that were PCR positive for pathogenic Leptospira.
Results
A set of samples subjected to capture with RNA probes covering the L. interrogans core genome resulted in 72 to 13,000-fold increase in pathogen reads when compared to standard sequencing without capture. A SNP analysis of the genomes sequenced from the biological samples using 273 Leptospira reference genome was then performed in order to determine the genotype of the infecting strain. For samples with sufficent coverage (19/20 samples with coverage > 8X), we could unambigously identify L. interrogans sv Icterohaemorrhagiae (14 samples), L. kirschneri sv Grippotyphosa (4 samples) and L. interrogans sv Pyrogenes (1 sample) as the infecting strain.
Conclusions
We obtained for most of our clinicall samples high quality genomic data at suitable coverage for confident core genome genotyping of the agent of leptospirosis. The ability to generate culture-free genomic data opens new opportunities to better understand the epidemiology and evolution of this fastidious pathogen.
New splice variants of VEGF as relevant targets for the treatment of Renal Cell Carcinoma
Authors: Montemagno Christopher , Durivault Jérôme , Gastaldi Cécile , Dufies Maeva , Vial Valérie , He Xingkang , Ambrosetti Damien , Négrier Sylvie , Bernhard Jean-Christophe , Borchiellini Delphine , Cao Yihai , Pagès Gilles 
Published: June 2022

Abstract
Background
The efficacy of anti-VEGF/VEGF receptors in the treatment of metastatic clear cell renal cell carcinoma (ccRCC) varies from patient to patient. Discovering the reasons for this variability could lead to the identification of relevant therapeutic targets. We have investigated the possibility of splicing events leading to new forms of VEGF that are less efficiently inhibited by anti-VEGF/VEGFR targeting the conventional forms.
Methods
In silico analysis identified the presence of an unknown splice acceptor in the last intron of the VEGF gene resulting in an insertion of 23 bases in VEGF mRNA. Such an insertion can occur in previously described splice variants of VEGF (VEGFXXX) and shift the open reading frame, leading to a change in the c-terminal part of VEGF. We investigated the role of the resulting new major form of VEGF, VEGF222NF, in physiological and pathological angiogenesis. We analyzed the expression of these new alternatively spliced forms in normal tissue and in a series of RCC cells by qPCR and ELISA. We generated experimental RCC in mice by implanting ccRCC cells overexpressing VEGF222NF. The experimental RCC were also treated with polyclonal anti-VEGF/NF antibodies. The relationship between plasmatic VEGF/NF levels and resistance to anti-VEGFR and survival was also investigated in a cohort of patients from the NCT00943839 clinical trial.
Results
VEGF222/NF stimulated endothelial cell proliferation and vascular permeability through activation of VEGFR2. Overexpression of VEGF222/NF stimulated proliferation and metastatic properties of RCC cells, whereas its downregulation resulted in cell death. RCC cells overexpressing VEGF222/NF generated aggressive experimental tumors that developed functional blood and lymphatic vessels. Anti-VEGFXXX/NF antibodies slowed the growth of experimental RCC by inhibiting tumor cell proliferation and the development of blood and lymphatic vessels. High plasmatic VEGFXXX/NF levels correlated with shorter survival and lower efficacy of anti-angiogenic drugs.
Conclusions
The existence of new VEGF isoforms has shed new light on the VEGF field.
An efficient method for Multiple UAV Cooperative Forest Fire Reconnaissance Task Assignment
Authors: Liu Xinlin , Jing Tian , Hou Linyi 
Published: June 2022

Abstract
In this paper, we present a three-layer centralized multiple unmanned aerial vehicles(UAVs) task assignment method in collaboration with satellites for forest fire reconnaissance task, based on Gaussian mixture model (GMM) and multidimensional 0-1 knapsack model. The goal of UAVs reconnaissance task assignment problem is to maximize the utilization of UAVs by the reasonable allocation of UAV group. Hence, it is taken as a combinatorial optimization problem in this paper. We first obtain the basic information of the fire field through the space-based infrared sensors carried by satellites. Then we fit the fire points distribution through expectation maximization algorithm (EM) based on GMM to obtain the initial position of each UAV. Finally, we consider the task assignment problem of UAVs as a multidimensional 0-1 knapsack problem and use the improved genetic algorithm (GA) inspired by the fireworks algorithm (FWA) with improved select operator and elite opposition-based learning strategy (SeEl-FWGA) to solve the UAVs task assignment problem. Finally, the proposed method is demonstrated and compared with other methods under the background of forest fires in Liangshan Prefecture, Sichuan Province. The simulation results show the superiority to the traditional genetic algorithm in terms of iteration speed and iteration results, and the high efficiency to solve the UAVs task assignment problem.
Multigene phylogeny and morphology of Ophiocordyceps macroascus sp. nov., a Zombie-ant fungus
Authors: Tang Dexiang , Xu Zhihong , Wang Yao , Wang Yuanbing , Tran Ngoc-Lan , Yu Hong 
Published: June 2022

Abstract
Ophiocordyceps unilateralis sensu lato is a common pathogenic fungus in ants. The ant manipulation behavior of the O. unilateralis species complex has attracted great attention, and many species of O. unilateralis complex have been described. However, there are likely many unknown species in the O. unilateralis complex. This study describes a new fungus species on Camponotus sp. collected from China and Vietnam. Ophiocordyceps macroascus sp. nov. is described based on morphology and phylogenetic evidence from five genes (nrSSU, nrLSU, tef-1α, rpb1, and rpb2). Ophiocordyceps macroascus was characterized by infected Camponotus sp. biting onto a leaf, multiple fertile cushions, perithecia immersed, asci wide and short, ascospores tapered at both ends, and conidiogenous cells cylindrical to lageniform. Both morphological observations and phylogenetic analyses support that this fungus is a distinct species in the core clade of O. unilateralis within the genus Ophiocordyceps. This study increased our knowledge of the colony, morphology and distribution in this group, and provided important taxonomic data further to explore the evolutionary relationship between fungi and hosts.
In Search of the Aplysia Immunome: an in silico Study
Authors: Kron Nicholas 
Published: June 2022

Abstract
The immune repertoires of mollusks beyond commercially important organisms such as the pacific oyster Crassostrea gigas or vectors for human pathogens like the bloodfluke planorb Biomphalaria glabrata are understudied. Despite being an important model for neural aging and the role of inflammation in neuropathic pain, the immune repertoire of Aplysia californica is poorly understood. Recent discovery of a neurotropic nidovirus in Aplysia has highlighted the need for a better understanding of the Aplysia immunome. To address this gap in the literature, the Aplysia reference genome was mined using InterProScan and OrthoFinder for putative immune genes. The Aplysia genome encodes orthologs of all critical components of the classical Toll-like receptor (TLR) signaling pathway. The presence of many more TLRs and TLR associated adapters than known from vertebrates suggest yet uncharacterized, novel TLR associated signaling pathways. Aplysia also retains many nucleotide receptors and antiviral effectors known to play a key role in viral defense in vertebrates. However, the absence of key antiviral signaling adapters MAVS and STING in the Aplysia genome suggests divergence from vertebrates and bivalves in these pathways. The resulting immune gene set of this in silico study provides a basis for interpretation of future immune studies in this important model organism.
Segmentation of Acute Pulmonary Embolism in Computed Tomography Pulmonary Angiography Using the Deep Learning Method
Authors: Aydin Nevin , Cihan Çağatay , Celik Ozer , Aslan Ahmet Faruk , Odabas Alper , Alatas Füsun , Yıldırım Hüseyin 
Published: June 2022

Abstract
Background
Pulmonary embolism is a type of thromboembolism seen in the main pulmonary artery and its branches. This study aimed to diagnose acute pulmonary embolism using the deep learning method in computed tomographic pulmonary angiography (CTPA) and perform the segmentation of pulmonary embolism data.
Methods
The CTPA images of patients diagnosed with pulmonary embolism who underwent scheduled imaging were retrospectively evaluated. After data collection, the areas that were diagnosed as embolism in the axial section images were segmented. The dataset was divided into three parts as training, validation, and testing. The results were calculated by selecting 50% as the cut-off value for the intersection over union.
Results
Images were obtained from 1,550 patients. The mean age of the patients was 64.23 ± 15.45 years. A total of 2,339 axial computed tomography images obtained from the 1,550 patients were used. There were a total of 5,992 labels, with 1,879 images. PyTorch U-Net was used to train 400 epochs, and the best model, epoch 178, was recorded. In the testing group, the number of true positives was determined as 471 and false positives as 35, while 27 were not detected. The sensitivity of CTPA segmentation was 0.95, the precision value was 0.93, the F1 score value was 0.94, and the learning rate was 0.0001. The area under the curve value obtained in the receiver operating characteristic analysis was calculated as 0.88.
Conclusions
In this study, the segmentation of acute pulmonary embolism in CTPA performed using the deep learning method provided successful results.
Importance and Challenges of Nursing Undergraduates' Ideological and Political and Psychological Education in the New Medical Era
Authors: Gao XiaoLei , Zhao Tong , Du MinXia , Li Juan , Sang WenFeng , Zhang ZhaoHui 
Published: June 2022

Abstract
Background: The university stage is an important period of life development. Ideological and political education and psychological education are crucial for schools to ensure the political direction of cultivating people. However, low teaching efficiency has been the common problem in education. The purpose of this study was to analyze the importance and to explore the challenges faced by those two kinds of education.Methods: First, the importance of ideological and political, and psychological education in specialty courses for nursing undergraduates was investigated and studied by literature research and a questionnaire survey. Additionally, we discussed the challenges of ideological and political, and psychological education faced in the New Medical era. Second, a total of 157 survey data were selected randomly from the nursing school of XX Medical University, and their attitudes toward ideological and political education in specialty courses were analyzed by a questionnaire survey partly through wenjuanxing online. Meanwhile, the opportunity and challenges undergraduates have encountered were revealed by analyzing the survey data. Results: The results show that most undergraduates accept ideological and political education and recognize its importance; however, there are also some problems in ideological and political education that need to be noticed and addressed. Approximately 54% of the respondents recognized the significance of ideological and political education in specialty courses for nursing undergraduates. Approximately 52% of the nursing students would like increased comprehensive quality in ideological and political education in specialty courses for nursing undergraduates, 47% of the respondents considered the teachers unable to interpret the knowledge simply, and 39% of them thought the class atmosphere was not good enough. Currently, approximately 70% of nursing undergraduates want to watch ideological and political content related to specialty nursing courses via the internet, which may be a new challenge for teaching.Conclusions: The current research enforced the importance of ideological and political and psychological education in the New Medical era of nursing undergraduates. Meanwhile, it provides a serious and useful reference for ideological and political and psychological education, which is beneficial for solving the problems in ideological and political and psychological education in specialty courses for nursing undergraduates.