bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pelaksanaan Pembelajaran Life Skill Menjahit Kelompok Belajar Paket C Di PKBM Mutiara Bintang
Authors: Rodiah Rodiah , Sofino Sofino , Abdullah Ilham 
Published: February 2022

PKBM Mutiara Bintang Kota Bengkulu merupakan salah satu lembaga yang menyelenggarakan pelaksanaan pembelajaran life skill menjahit bagi kelompok belajar paket C pada mata pelajaran muatan lokal dengan bertujuan meningkatkan kemampuan keterampilan menjahit. Penelitian ini bertujuan untuk mengetahui Pelaksanaan pembelajaran life skill menjahit mulai dari perencanaan pembelajaran yang telah dibuat oleh pihak lembaga, proses pembelajaran dari segi kegiatan belajar, faktor penghambat dan faktor pengdukung dalam pelaksanaan pembelajaran, evaluasi pelaksanaan pembelajaran dan keberhasilan hasil belajar dalam pelaksanaan pembelajaran life skill menjahit bagi kelompok belajar. Penelitian dilakukan dengan menggunakan metode kualitatif. Data yang dikumpulkan teknik wawancara, observasi dan dokumentasi. Hasil penelitian pelaksanaan pembelajaran life skill menjahit bagi kelompok belajar paket C yaitu diawali dengan rekrutmen kelompok belajar, persiapan kurikulum,materi,metode dan media dan proses pembelajaran yang dilaksanakan dua kali dalam seminggu dan faktor pengdukung dari segi sarana prasarana dan evaluasi dilakukan dengan dilihat dari hasil belajar paket C dan keberhasilan hasil belajar dilihat dari pembuatan tutup untuk (kulkas,galon dan magicom) dan sarung bantal.
Evaluation of the influence of calcium chloride on the behavior of phases of nanosystems applied in the larvicidal control of Aedes aegypti
Authors: Santos Adriana de Jesus , Macêdo Nathália Araújo , Mendonça Marcos Rafael das Chagas , Cavalcanti Sócrates Cabral de Holanda , Sarmento Victor Hugo Vitorino , Lira Ana Amélia Moreira , Araujo Guilherme Rodolfo Souza de , Santos Cochiran Pereira dos , Souza Roberto Rodrigues de , Nunes Rogéria de Souza 
Published: February 2022

Systems stabilized by surfactants in order to incorporate different substances at their interfaces, such as hydrophilic polymers and active compounds, are systems capable of establishing vector control of Aedes aegypti. The system was obtained through the surface tension of PPG-5-CETETH-20/calcium chloride dispersion at concentrations of 0.2%, 0.4%, 0.8%, 1% and 2%, determining the CMC of each sample. Then, a binary study was performed and the system structures were evaluated by Polarized Light Microscopy (PLM) and rheological analysis. Biological analysis was performed to assess the mean lethal concentration (LC50) against Aedes aegypti larvae and toxicity to non-target organisms in Artemia salina. In this sense, the objective of this work was to evaluate the influence of calcium chloride on the behavior of phases and on the biological activity of formulations based on the self-aggregation of surfactants, an alternative larval insecticide to be used as a replacement for larval insecticides already on the market.
Protecting the Consumer Rights in the Digital Economic Era: Future Challenges in Indonesia
Authors: Arifin Ridwan , Kambuno Juan Anthonio , Waspiah Waspiah , Latifiani Dian 
Published: February 2022

The emerging technological prospects affected many aspects, including consumer protection. The creation of data and computer-driven innovation would derive several advantages for consumers. Innovative products and services and new ways in which goods and services are developed and delivered. This paper aims to analyse how Indonesian legal policy on protection consumer rights in the digital era. The paper emphasized that it would advise against believing that policy is unreasonably permitted to give way to the technological agenda, whilst accepting that adaptations should be made and also that there should be a critical review of whether existing forms of regulation are needed in the digital economy of Indonesia. Regulations concerning to the protecting consumer rights in the world of e-commerce are still worrying even though many sectoral regulations have been promulgated, as well as weak supervision and lack of strict law enforcement in resolving consumer disputes. Trade regulation via the internet still raises many conceptual or theoretical questions that indicate the need to develop more detailed conceptual and theoretical explanations to the need for more complex laws and regulations to be able to protect consumers properly. More than that, national regulations are dealing with the context of trade through the internet, which transcends national boundaries without always being controlled through conventional means.
Isolation and Identification of Polyphenols From Fresh Sweet Sorghum Stems and Their Antibacterial Mechanism Against Foodborne Pathogens
Authors: Chen Hao , Xu Yifei , Chen Haoyu , Liu Hao , Yu Qunli , Han Ling 
Published: February 2022

As a C4 energy crop widely planted all over the world, sweet sorghum is mainly used in sugar making and brewing. Fresh sweet sorghum stalks contain many natural ingredients that have antioxidant properties and can significantly inhibit the growth of foodborne pathogens. In this study, the polyphenols in sweet sorghum were extracted by acid ethanol and ion precipitation, and the types of polyphenols were determined by HPLC-MS. The polyphenol content in fresh sweet sorghum stalks was 5.77 mg/g after process optimization with 18 types of phenolic acids identified. The extract had a total antioxidant capacity of 9.4 μmol Trolox/mL. Polyphenol extract of sweet sorghum displayed antibacterial activity against Staphylococcus aureus, Escherichia coli, Listeria spp., and Salmonella spp. The extract increased the conductivity of cell suspensions by destroying the membrane structure, resulting in leakage of cell electrolytes. Changes in bacterial morphology and internal structure were indicated. The data describe an optimized process to extract polyphenols from sweet sorghum stalks and the methodology to identify the major components within the extract. The data provide a novel option for the comprehensive utilization of fresh sweet sorghum stalks.
Biochemical and Molecular Dynamics Study of a Novel GH 43 α-l-Arabinofuranosidase/β-Xylosidase From Caldicellulosiruptor saccharolyticus DSM8903
Authors: Saleh Md. Abu , Mahmud Shafi , Albogami Sarah , El-Shehawi Ahmed M , Paul Gobindo Kumar , Islam Shirmin , Dutta Amit Kumar , Uddin Md. Salah , Zaman Shahriar 
Published: February 2022

The complete hydrolysis of xylan can be facilitated by the coordinated action of xylanase and other de-branching enzymes. Here, a GH43 α-l-arabinofuranosidase/β-xylosidase (CAX43) from Caldicellulosiruptor saccharolyticus was cloned, sequenced, and biochemically investigated. The interaction of the enzyme with various substrates was also studied. With a half-life of 120 h at 70°C, the produced protein performed maximum activity at pH 6.0 and 70°C. The enzyme demonstrated a higher activity (271.062 ± 4.83 U/mg) against para nitrophenol (pNP) α-L-arabinofuranosides. With xylanase (XynA), the enzyme had a higher degree of synergy (2.30) in a molar ratio of 10:10 (nM). The interaction of the enzyme with three substrates, pNP α-L-arabinofuranosides, pNP β-D-xylopyranosides, and sugar beet arabinan, was investigated using protein modeling, molecular docking, and molecular dynamics (MD) simulation. During the simulation time, the root mean square deviation (RMSD) of the enzyme was below 2.5 Å, demonstrating structural stability. Six, five, and seven binding-interacting residues were confirmed against pNP α-L-arabinofuranosides, pNP β-D-xylopyranosides, and arabinan, respectively, in molecular docking experiments. This biochemical and in silico study gives a new window for understanding the GH43 family’s structural stability and substrate recognition, potentially leading to biological insights and rational enzyme engineering for a new generation of enzymes that perform better and have greater biorefinery utilization.
Angiogenesis, Osseointegration, and Antibacterial Applications of Polyelectrolyte Multilayer Coatings Incorporated With Silver/Strontium Containing Mesoporous Bioactive Glass on 316L Stainless Steel
Authors: Kuo Yi-Jie , Chen Chia-Hsien , Dash Pranjyan , Lin Yu-Chien , Hsu Chih-Wei , Shih Shao-Ju , Chung Ren-Jei 
Published: February 2022

The main causes for failure in implant surgery are prolonged exposure of implants or wound and tissue ischemia. Bacterial infection caused by the surrounding medical environment and equipment is also a major risk factor. The medical risk would be greatly reduced if we could develop an implant coating to guide tissue growth and promote antibacterial activity. Mesoporous bioactive glasses are mainly silicates with good osteoinductivity and have been used in medical dentistry and orthopedics for several decades. Strontium ions and silver ions could plausibly be incorporated into bioactive glass to achieve the required function. Strontium ions are trace elements in human bone that have been proposed to promote osseointegration and angiogenesis. Silver ions can cause bacterial apoptosis through surface charge imbalance after bonding to the cell membrane. In this study, functional polyelectrolyte multilayer (PEM) coatings were adhered to 316L stainless steel (SS) by spin coating. The multilayer film was composed of biocompatible and biodegradable collagen as a positively charged layer, γ-polyglutamic acid (γ-PGA) as a negatively charged layer. Chitosan was incorporated to the 11th positively charged layer as a stabilizing barrier. Spray pyrolysis prepared mesoporous bioactive glass incorporated with silver and strontium (AgSrMBG) was added to each negatively charged layer. The PEM/AgSrMBG coating was well hydrophilic with a contact angle of 37.09°, hardness of 0.29 ± 0.09 GPa, Young’s modulus of 5.35 ± 1.55 GPa, and roughness of 374.78 ± 22.27 nm, as observed through nano-indention and white light interferometry. The coating’s antibacterial activity was sustained for 1 month through the inhibition zone test, and was biocompatible with rat bone marrow mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs), as observed in the MTT assay. There was more hydroxyapatite precipitation on the PEM/AgSrMBG surface after being soaked in simulated body fluid (SBF), as observed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). In both in vitro and in vivo tests, the PEM/AgSrMBG coating promoted angiogenesis, osseointegration, and antibacterial activity due to the sustained release of silver and strontium ions.
Playful Communication and Care: Exploring Child-Centred Care of Young Children With Type 1 Diabetes Through the Framework of Zone of Proximal Development
Authors: DeCosta Patricia , Grabowski Dan , Jespersen Louise Norman , Skinner Timothy C. 
Published: February 2022

IntroductionLittle is known about the psychosocial experiences and care needs of young children under the age of 7 years who have been diagnosed with type 1 diabetes. To address this knowledge gap, we examine children’s psychosocial care needs through the lens of child-centred care and the framework of Zone of Proximal Development.ObjectivesTo explore current care practices for young children with diabetes and identify aspects of child-centred care already successfully integrated into current practice.MethodIndividual face-to-face, semi-structured interviews were conducted with 20 Healthcare Professionals, representing 11 of 17 paediatric diabetes clinics in Denmark.ResultsOur data provided valuable insights into existing child-centred practices. Our analysis identified practices covering four main themes: 1. Accommodating immediate emotional needs, 2. Putting children before diabetes, 3. Encouraging meaningful participation, 4. Playful communication.DiscussionHealthcare Professionals provided child-centred care, largely through play-based approaches that make diabetes care meaningful and relevant. Such practices provide the scaffolding necessary to enable young children to gradually engage, comprehend and participate in their own care.
A Prediction Model for Acute Kidney Injury After Pericardiectomy: An Observational Study
Authors: Wang Jin , Yu Chunhua , Zhang Yuelun , Huang Yuguang 
Published: February 2022

ObjectivesAcute kidney injury is a common complication after pericardiectomy for constrictive pericarditis, which predisposes patients to worse outcomes and high medical costs. We aimed to investigate potential risk factors and consequences and establish a prediction model.MethodsWe selected patients with constrictive pericarditis receiving isolated pericardiectomy from January 2013 to January 2021. Patients receiving concomittant surgery or repeat percardiectomy, as well as end-stage of renal disease were excluded. Acute kidney injury was diagnosed and classified according to the KDIGO criteria. Clinical features were compared between patients with and without postoperative acute kidney injury. A prediction model was established based on multivariable regression analysis.ResultsAmong two hundred and eleven patients, ninety-five (45.0%) developed postoperative acute kidney injury, with fourty-three (45.3%), twenty-eight (29.5%), and twenty-four (25.3%) in mild, moderate and severe stages, respectively. Twenty-nine (13.7%) patients received hemofiltration. Nine (4.3%) patients died perioperatively and were all in the acute kidney injury (9.5%) group. Eleven (5.2%) patients were considered to have chronic renal dysfunction states at the 6-month postoperative follow-up, and eight (72.7%) of them experienced moderate to severe stages of postoperative acute kidney injury. Univariable analysis showed that patients with acute kidney injury were older (difference 8 years, P &lt; 0.001); had higher body mass index (difference 1.68 kg·m−2, P = 0.002); rates of smoking (OR = 2, P = 0.020), hypertension (OR = 2.83, P = 0.004), and renal dysfunction (OR = 3.58, P = 0.002); higher central venous pressure (difference 3 cm H2O, P &lt; 0.001); and lower cardiac index (difference −0.23 L·min−1·m−2, P &lt; 0.001) than patients without acute kidney injury. Multivariable regression analysis showed that advanced age (OR 1.03, P = 0.003), high body mass index (OR 1.10, P = 0.024), preoperative atrial arrhythmia (OR 3.12, P = 0.041), renal dysfunction (OR 2.70 P = 0.043), high central venous pressure (OR 1.12, P = 0.002), and low cardiac index (OR 0.36, P = 0.009) were associated with a high risk of postoperative acute kidney injury. A nomogram was established based on the regression results. The model showed good model fitness (Hosmer-Lemeshow test P = 0.881), with an area under the curve value of 0.78 (95% CI: 0.71, 0.84, P &lt; 0.001).ConclusionThe prediction model may help with early recognition, management, and reduction of acute kidney injury after pericardiectomy.
Correlation Between Smoking Paradox and Heart Rhythm Outcomes in Patients With Coronary Artery Disease Receiving Percutaneous Coronary Intervention
Authors: Wu Han-Ping , Jan Sheng-Ling , Chang Shih-Lin , Huang Chia-Chen , Lin Mao-Jen 
Published: February 2022

BackgroundThe effect of smoking on short-term outcomes among patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI) is controversial. However, little is known about the impact of smoking on long-term outcomes in patients with stable coronary artery disease (CAD) who receive PCI.MethodsA total of 2,044 patients with stable CAD undergoing PCI were evaluated. They were divided into two groups according to smoking status (current smokers vs. non-smokers). Baseline characteristics, exposed risk factors, angiographic findings, and interventional strategies were assessed to compare the long-term clinical outcomes between groups. Predictors for myocardial infarction (MI), all-cause death, cardiovascular (CV) death, and repeated PCI procedures were also analyzed.ResultsCompared with non-smokers, current smokers were younger and mostly male (both P &lt; 0.01). They also had a lower prevalence of chronic kidney disease (CKD) and diabetes (both P &lt; 0.01). Drugs including a P2Y12 receptor inhibitor of platelets (P2Y12 inhibitor), beta-blockers (BB), and statins were used more frequently in current smokers (P &lt; 0.01, P &lt; 0.01, P = 0.04, respectively). Freedom from all-cause death and CV death was lower in the non-smoker group (P &lt; 0.001, P = 0.003, respectively). After adjustment, logistic regression revealed smoking was a major predictor for all-cause death and repeated PCI procedure [hazard ratio(HR): 1.71 and 1.46, respectively].ConclusionsSmoker's paradox extends to long-term outcome in patients with stable CAD undergoing PCI, which is partially explained by differences in baseline characteristics. However, smoking strongly predicted all-cause mortality and repeated PCI procedures in patients with stable CAD undergoing PCI.
Experimental Study on Thermal Regime and Frost Jacking of Pile Foundation During Operation Period in Permafrost Regions
Authors: Shang Yunhu , Niu Fujun , Fang Jianhong , Wu Libo 
Published: February 2022

The stability of a cast-in-place pile foundation in permafrost region is primarily subject to the thermal regime and tangential frost-heave forces (TFF) during the operation period. However, studies focusing on the thermal and mechanical characteristics of pile foundation during runtime are rare. To investigate the effect of pile foundation on the thermal regime and quantify the magnitude of unit tangential frost-heave forces (UTFF), a field experiment was conducted on the Qinghai–Tibet Plateau, China. Results showed that the cast-in-place pile foundation enhanced the heat exchange between the atmosphere and soil, which expanded the annual range of the surrounding ground temperature. Furthermore, the permafrost table depth was increased by 0.4–0.7 m (0.33–0.58 times the pile diameter). The TFF increased significantly when the soil temperature decreased from 0 to −0.5°C. Meanwhile, the thickness of the frost heaving layer was approximately double that of the active layer, in which the maximum UTFF was higher than 52.04 kPa. The adfreezing bond force of permafrost to pile shaft burdened most of the applied load, and the end bearing contributed relatively little. Findings from this study will benefit the stability maintenance and structure design of pile foundation in permafrost regions.
J-Shaped Relationship Between Serum Prolactin and Metabolic-Associated Fatty Liver Disease in Female Patients With Type 2 Diabetes
Authors: Zhu Cuiling , Ma Huihui , Huang Dongdong , Li Guifang , Gao Jingyang , Cai Meili , You Hui , Bu Le , Qu Shen 
Published: February 2022

BackgroundMetabolic-associated fatty liver disease (MAFLD) has become a worldwide epidemic. Prolactin (PRL), a pituitary hormone, has been linked to MAFLD. As a result, we set out to look into the relationship between serum PRL and the risk of MAFLD in patients with type 2 diabetes mellitus (T2DM).MethodsA total of 724 adults with T2DM were enrolled and categorized as MAFLD and non-MAFLD groups. Anthropometric data, biochemical parameters, and serum PRL levels were collected. Liver steatosis and fibrosis were assessed using FibroScan. Patients were stratified into normal PRL (NP) and high PRL (HP) groups and divided into four groups based on serum PRL quartiles. Multivariate logistic regression analysis was performed to evaluate the association between serum PRL and MAFLD risk.ResultsFemale but not male patients with MAFLD, liver steatosis, and fibrosis had significantly lower PRL levels in the NP group but higher PRL levels in the HP group than their counterparts. The proportions of MAFLD, liver steatosis, and fibrosis were significantly decreased in the NP group but increased in the HP group across the PRL quartiles in females but not in males. After multivariate adjustment, the adjusted ORs (AORs) and 95% CI for MAFLD among females were 18.165 (3.425–96.336), 1.784 (0.658–5.002), 1.744 (0.608–4.832), and 1.00 (reference) in the NP group (Q1–Q4, P-trend &lt; 0.001) and 1.00 (reference), 11.098 (1.819–110.356), 15.225 (1.996–116.112), and 18.211 (2.579–128.568) in the HP group (Q1–Q4, P-trend = 0.020). Such associations were also found between serum PRL and liver fibrosis in females but not in males.ConclusionWe observed a J-shaped association between serum PRL and the risk of MAFLD and liver fibrosis in females but not in males with T2DM, indicating that PRL may be relevant to MAFLD and its progression in a gender-specific manner.Clinical Trial RegistrationChinese Clinical Trial Registry, number ChiCTR-OCS-12002381.
Dental and Orthopaedic Implant Loosening: Overlap in Gene Expression Regulation
Authors: Schluessel Sabine , Hartmann Eliza S. , Koehler Miriam I. , Beck Felicitas , Redeker Julia I. , Saller Maximilian M. , Akova Elif , Krebs Stefan , Holzapfel Boris M. , Mayer-Wagner Susanne 
Published: February 2022

ObjectivesEndoprosthetic loosening still plays a major role in orthopaedic and dental surgery and includes various cellular immune processes within peri-implant tissues. Although the dental and orthopaedic processes vary in certain parts, the clinical question arises whether there are common immune regulators of implant loosening. Analyzing the key gene expressions common to both processes reveals the mechanisms of osteoclastogenesis within periprosthetic tissues of orthopaedic and dental origin.MethodsDonor peripheral blood mononuclear cells (PBMCs) and intraoperatively obtained periprosthetic fibroblast-like cells (PPFs) were (co-)cultured with [± macrophage-colony stimulating factor (MCSF) and Receptor Activator of NF-κB ligand (RANKL)] in transwell and monolayer culture systems and examined for osteoclastogenic regulations [MCSF, RANKL, osteoprotegerin (OPG), and tumor necrosis factor alpha (TNFα)] as well as the ability of bone resorption. Sequencing analysis compared dental and orthopaedic (co-)cultures.ResultsMonolayer co-cultures of both origins expressed high levels of OPG, resulting in inhibition of osteolysis shown by resorption assay on dentin. The high OPG-expression, low RANKL/OPG ratios and a resulting inhibition of osteolysis were displayed by dental and orthopaedic PPFs in monolayer even in the presence of MCSF and RANKL, acting as osteoprotective and immunoregulatory cells. The osteoprotective function was only observed in monolayer cultures of dental and orthopaedic periprosthetic cells and downregulated in the transwell system. In transwell co-cultures of PBMCs/PPFs profound changes of gene expression, with a significant decrease of OPG (20-fold dental versus 100 fold orthopaedic), were identified. Within transwell cultures, which offer more in vivo like conditions, RANKL/OPG ratios displayed similar high levels to the original periprosthetic tissue. For dental and orthopaedic implant loosening, overlapping findings in principal component and heatmap analysis were identified.ConclusionsThus, periprosthetic osteoclastogenesis may be a correlating immune process in orthopaedic and dental implant failure leading to comparable reactions with regard to osteoclast formation. The transwell cultures system may provide an in vivo like model for the exploration of orthopaedic and dental implant loosening.