bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Romanov breed lamb gender on carcass traits and meat quality parameters including biogenic amines and malondialdehyde changes during storage
Authors: Klupsaite Dovile , Buckiuniene Vilija , Bliznikas Saulius , Sidlauskiene Sonata , Dauksiene Agila , Klementaviciute Jolita , Jurkevicius Andrius , Zaborskiene Gintare , Bartkiene Elena 
Published: March 2022

AbstractThe present study aims to evaluate the effect of Romanov breed lamb gender on carcass traits and meat quality parameters, as well as on the formation of biogenic amines (BAs) and malondialdehyde during meat storage. Obtained results revealed that lamb gender had a significant influence on sternum/breastbone, ribs, right shoulder, and bones of the back leg. Significantly higher lightness (by 3%) was found for male meat; however, higher redness of female meat was observed (by 7.7%). In all cases, a lower pH was obtained for female meat. Significantly higher cooking loss (by 38%) was found for male meat. However, gender was not a significant factor in lamb meat proximate composition, or for BAs and cholesterol content. The gender of animals had a significant influence on 10‐heptadecenoic (C17:1), linoleic (C18:2n – 6), total polyunsaturated FA, and total trans isomers content in meat. A significantly higher concentration of malondialdehyde was found in female lamb meat (by 43.4% and 56.8% in fresh and after 3 months of storage at −18°C, respectively) compared to males. Finally, the obtained results supplement the scarce database about the characteristics of Romanov breed meat of different gender and this is beneficial for lamb breeders and meat industry in order to obtain a better quality production.
Elaboration of an organic beverage based on grape juice with positive nutritional properties
Authors: Bendaali Yasmina , Vaquero Cristian , González Carmen , Morata Antonio 
Published: March 2022

AbstractThe present study aimed to develop a natural beverage with interesting phytochemical composition and biological activity based on grape juice without added sugars or artificial additives. Two groups of blends were made by diluting concentrate grape juice with a sugar content of 65 °Brix with two different mineral waters (BA: Bezoya with low mineralization; BB: Solan de Cabras with high mineralization). Lemon juice was used for pH correction, and mixtures of extractions of hop with tea and hop with mint were used to increase aroma. Samples were stored under refrigeration (4°C) then subjected to physicochemical and sensory analysis. The results demonstrated that malvidin‐3‐O‐glucoside pigment was the predominant pigment with a concentration ranging from 75.71 ± 12.49 to 84.87 ± 1.70 mg/L. The levels of sugars ranged from 79.90 ± 1.37 to 82.37 ± 0.55 g/L and total soluble solids were between 5.47 ± 0.12 and 5.77 ± 0.06 °Brix. Total acids presented a significant difference, ranging from 1.40 ± 0.00 to 1.43 ± 0.06 g/L in BA samples and from 1.10 ± 0.10 to 1.20 ± 0.00 g/L in BB samples. For 20 days, the color increased in all beverages. However, BA drinks presented higher acidity and higher red color intensity than BB drinks, so the type of water and pH influenced the color of beverages. The sensory evaluation showed that the beverage made with low mineral water and flavored with a mixture of hop with tea was preferred.
Stability and variability of long‐term streamflow and its components in watersheds under vegetation restoration on the Chinese Loess Plateau
Authors: Zhang Xiaoping , Yi Haijie , Xue Fan , Bruijnzeel Leendert Adrian , Cheng Zhuo , Liu Baoyuan 
Published: March 2022

AbstractAssessing ecological restoration effects on hydrological regimes is important for water resources management, especially under semi‐arid conditions. Here, the stability and long‐term variability of streamflow components were analyzed for three less forested watersheds (LFWs, vegetation coverage 16–23% in 1970) undergoing major vegetation restoration (57–80% coverage in 2019), and for two largely forested watersheds (FWs, 65–68% cover in 1970 increasing to 88–92% in 2019) within the Beiluo River Basin on the Chinese Loess Plateau. Daily flow data for each watershed and year were normalized by rainfall to eliminate effects of non‐stationary rainfall. Mean annual streamflow totals for the ~60‐year study period were 25.1–34.1 and 21.6–48.1 mm y−1 for the LFWs and FWs, respectively. Average contributions of baseflow to total streamflow were 32–44% for the LFWs and 58–61% for the FWs. Mann‐Kendall tests showed significant decreasing trends for annual streamflow and stormflow (0.23–0.54% y−1) from the LFWs throughout. Mean streamflow from the LFWs between 2000 and 2019 decreased by 58% compared to the pre‐2000 period, while the average contribution of stormflow decreased from 66% to 35% (2010s). However, winter baseflow increased with time since start of restoration. Conversely, streamflow (components) for the FWs showed only slight fluctuations in decadal, annual and seasonal trends. Both total streamflow and stormflow exhibited strong power relationships with vegetation coverage, illustrating a tendency towards stable flow regimes for vegetation coverage >60–70%. Large‐scale vegetation restoration has fundamentally changed amounts and temporal distribution of streamflow (components) on the Loess Plateau mainly by regulating stormflow. After 20 years of restoration, the trend and proportion of streamflow components from the LFWs approximated those of the FWs. These results underpin the planning of sustainable management of natural resources vis‐á‐vis socio‐economic development during long‐term ecological restoration on the Loess Plateau.
Describing correlates of early childhood screen time and outdoor time in Soweto, South Africa
Authors: Prioreschi Alessandra , Norris Shane A. 
Published: March 2022

AbstractBackgroundContextual factors are likely to influence whether young children are able to adhere to recommended health behaviours. This study aimed to: (1) describe the social and environmental characteristics of children under five living in Soweto, South Africa; and (2) determine factors associated with screen time and outdoor play in this population.MethodsHousehold surveys were conducted in Soweto to collect data on children's screen time and access to outdoor space, as well as information about the household. A multilevel regression analysis was conducted for each outcome.ResultsData on 2309 children aged five or under were included in this analysis. Children used screens for an average of one and a half hours per day during the week, and nearly 2 hours per day on weekends. Almost all (92%) children had a safe space to play inside, while just over a third (34%) had a safe space to play outside. A higher socioeconomic status was associated with less time spent playing outside and more screen time.ConclusionInterventions promoting outdoor play and restricting screen time are essential for improving health trajectories, but need to address structural barriers that exist, in order to protect the safety of children while promoting health behaviours.
Hapten sensitization to vaginal mucosa induces less recruitment of dendritic cells accompanying TGF‐β‐expressing CD206+ cells compared with skin
Authors: Nakayama Kanako , Nishijo Taku , Miyazawa Masaaki , Watabe Tetsuro , Azuma Miyuki , Sakaguchi Hitoshi 
Published: March 2022

AbstractIntroductionContact hypersensitivity (CHS), a type of delayed‐type hypersensitivity, is induced by hapten exposure to the skin and mucosa. We previously reported that, in a murine model of CHS, the vaginal mucosa (VM) sensitization showed lower T‐cell responses as compared with the abdominal skin sensitization. To investigate mechanisms of impaired CHS by the VM sensitization, we compared migration of hapten‐captured dendritic cells (DCs) in the draining lymph nodes (dLNs) and recruitment of DCs at the sensitized local sites.MethodsFluorescein isothiocyanate (FITC) or 2,4‑dinitrofluorobenzene (DNFB) was used as hapten, and migration of FITC+ DCs in the dLNs and local recruitment of MHC class II+ and CD11c+ cells were compared between abdominal skin and VM sensitization by flow cytometric analyses and immunohistochemistry. Expression of tumor growth factor (TGF)‐β at mRNA and protein levels, and local recruitment of CD206+ cells were examined after VM sensitization.ResultsVM sensitization showed less numbers of FITC+MHC class IIhighCD11c+ migratory DCs in the dLNs at 6 and 24 h, as compared with skin sensitization. Both skin and VM sensitization induced the recruitment of dermal/submucosal DCs at 6 h, but the number of submucosal DCs in the VM was significantly decreased at 24 h. VM showed persistently higher mRNA levels of TGF‐β2/β3 expression than those of the skin before and after sensitization. In the VM sensitization, increment of CD206+MHC class II+ cells was observed especially at the deep lamina propria at 24 h. Most of CD206+ cells were also positive for the binding to Fc chimeric TGF‐β receptor that interacts with all TGF‐β isoforms, suggesting TGF‐β expression.ConclusionDC migration to dLNs and localization of DCs at the sensitized sites are limited in the VM sensitization. Our results suggest that the existence of TGF‐β‐expressing CD206+ cells may contribute less sensitization ability and CHS responses in the VM.
A review of the efficacy of easily accessible dry decontaminants for human chemical contamination
Authors: Nandamuri Saisha , Feschuk Aileen M. , Maibach Howard I. 
Published: March 2022

AbstractSoap and water are often considered the gold standard for dermal decontamination. However, recent systematic reviews have shown that these methods often result in incomplete decontamination and may even induce contaminant absorption due to the “wash‐in” effect. Therefore, it is important to gain insight on other decontamination methods. A literature search was done using PubMed to find experimental studies relating to dry decontamination performed with readily available items. Seven studies met eligibility criteria, and the study model, dry decontaminant, method of dry decontamination, method of analyzing decontamination, and main conclusions from each study were extracted, summarized, and compared. Important conclusions include that all studies investigated found that dry decontamination yielded decreases in contamination. In addition, it was shown by multiple studies that not only the decontaminant, but the manner in which it is used (method used [blotting, rubbing, etc.], amount used, and whether decontamination instructions are provided to exposed individuals) is vital to success. Finally, in all four studies that investigated wet and dry decontamination combination protocols, combinations were more efficacious than dry decontamination alone. However, this means that dry improvised decontamination can be performed while waiting for the deployment and arrival of further formal decontaminants. These conclusions deserve consideration in the event that universal decontamination guidelines are designed. However, more studies are required in order to draw definitive conclusions regarding the important topic of dermal decontamination.
Alinity hq platelet count is not impacted by severe microcytosis
Authors: Lakos Gabriella , Mukhtar Zainab , Masi Loredana , Valente Sabatino , Papa Fabrizio 
Published: March 2022

AbstractBackgroundImpedance technology has been shown to overestimate platelet (PLT) count in samples with microcytes, while the optical‐fluorescence PLT count (PLT‐F) by Sysmex has been suggested to be unaffected by microcytosis. The Abbott Alinity hq analyzer employs multi‐dimensional optical PLT counting. Our goal was to assess the accuracy of this technology in microcytic samples.MethodsPlatelet measurements were performed by Alinity hq and the impedance (PLT‐I) and PLT‐F methods on a Sysmex XN‐3000 analyzer on 464 samples. PLT concentration range was 6.56–947 × 109/L and mean cell volume (MCV) 40.9–123.0 fL. Samples were categorized into normocytic (MCV > 80 fL), microcytic (MCV 65–80 fL), and severely microcytic (MCV < 65 fL) groups.ResultsAlinity hq PLT count showed excellent agreement with PLT‐F (r = 1.00). Sysmex PLT‐I data showed somewhat weaker correlation with both PLT‐F and Alinity hq (r = 0.98). Increasing bias between Sysmex PLT‐I and PLT‐F was seen with decreasing MCV values, with mean bias of 35.2 × 109/L in severe microcytosis. An inverse relationship was demonstrated between the PLT‐I versus PLT‐F bias and MCV (p < 0.0001). Consistent mean bias was observed between Alinity hq and PLT‐F across all MCV ranges.Mean platelet volume was suppressed or flagged by Sysmex XN in 50% of the samples in the severely microcytic group, and markedly higher red cell distribution width (RDW) was reported compared to Alinity hq (18.1% vs 13.7%, p < 0.0001).ConclusionThe Sysmex PLT‐I method overestimated the PLT count in samples with severe microcytosis. Alinity hq provided PLT counts and PLT and RBC indices that were not impacted by microcytosis.
hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR‐217 to upregulate anterior gradient 2
Authors: Lv Yumei , Wang Mingyi , Chen Mingli , Wang Dan , Luo Mingyan , Zeng Qingyuan 
Published: March 2022

AbstractBackgroundMounting evidence summarizes that circRNA is closely implicated in the development of numerous cancers. Our study aimed to investigate the role of circ_0119412 whose function was not explored in cervical cancer.MethodsRT‐qPCR analysis was utilized for the expression analysis of circ_0119412, miR‐217, and anterior gradient 2 (AGR2). CCK‐8 assay, transwell assay, and MTT assay were employed to assess cell proliferation, migration, and adhesion, respectively. Animal study was performed to check the role of circ_0119412 in vivo. Bioinformatics analysis was applied to predict the downstream targets of circ_0119412. RIP assay was utilized to examine miRNAs potentially bound by circ_0119412. The interplays between miR‐217 and circ_0119412 or AGR2 were validated by dual‐luciferase reporter assay.Resultscirc_0119412 expression was highly enhanced in cervical tumor tissues and cancer cells. circ_0119412 overexpression aggravated cervical cancer cell proliferation, migration, and adhesion, and its overexpression was also conducive to tumor formation and growth in animal models. AGR2 was upregulated in cervical cancer by the public bioinformatics data. circ_0119412 bound to miR‐217, and miR‐217 bound to AGR 3’UTR. The promoting effects of circ_0119412 overexpression on cancer cell malignant phenotypes were reversed by miR‐217 enrichment. In addition, increased expression of miR‐217 suppressed AGR2 expression, thus weakening the functional effects of AGR2.Conclusioncirc_0119412 functioned as an oncogenic driver to promote the malignant development of cervical cancer by targeting the miR‐217/AGR2 pathway.
Characterization of microchannel plate detector response for the detection of native multiply charged high mass single ions in orthogonal‐time‐of‐flight mass spectrometry using a Timepix detector
Authors: Mathew Anjusha , Eijkel Gert B. , Anthony Ian G. M. , Ellis Shane R. , Heeren Ron M. A. 
Published: March 2022

AbstractTime‐of‐flight (TOF) systems are one of the most widely used mass analyzers in native mass spectrometry (nMS) for the analysis of non‐covalent multiply charged bio‐macromolecular assemblies (MMAs). Typically, microchannel plates (MCPs) are employed for high mass native ion detection in TOF MS. MCPs are well known for their reduced detection efficiency when impinged by large slow moving ions. Here, a position‐ and time‐sensitive Timepix (TPX) detector has been added to the back of a dual MCP stack to study the key factors that affect MCP performance for MMA ions generated by nMS. The footprint size of the secondary electron cloud generated by the MCP on the TPX for each individual ion event is analyzed as a measure of MCP performance at each mass‐to‐charge (m/z) value and resulted in a Poisson distribution. This allowed us to investigate the dependency of ion mass, ion charge, ion velocity, acceleration voltage, and MCP bias voltage on MCP response in the high mass low velocity regime. The study of measurement ranges; ion mass = 195 to 802,000 Da, ion velocity = 8.4 to 67.4 km/s, and ion charge = 1+ to 72+, extended the previously examined mass range and characterized MCP performance for multiply charged species. We derived a MCP performance equation based on two independent ion properties, ion mass and charge, from these results, which enables rapid MCP tuning for single MMA ion detection.
Effect of enteral nutrition on the intestinal microbiome and risk of death in ischemic stroke patients
Authors: Tian Xiaolin , Xia Genghong , Zhang Mingsi , Tan Chuhong , Yang Mengjia , Wang Huidi , Song Xinna , Chai Shiqi , Yin Jia , Song Wei 
Published: March 2022

AbstractBackgroundStudies have shown that the intestinal microbiome of stroke patients is significantly altered and that the degree of microbiota disturbance correlates with prognosis. Enteral nutrition (EN) can reshape the intestinal microbiome and is important for stroke patients with dysphagia. We aimed to describe the intestinal microbiome in patients with ischemic cerebral infarction receiving standard EN.MethodsFirst, 17 healthy controls (HCs), 54 stroke patients with oral feeding (ON), and 50 stroke patients with EN were matched to investigate the changes in the intestinal microbiota with EN in the first week after admission and dynamic changes in the EN group in the second week. Second, we investigated the relationship between the intestinal microbiome and clinical characteristics in a larger sample of participants receiving EN (n = 147). Survival analysis was performed using Cox proportional hazards regression. The composition and structure of the intestinal microbiota were analyzed by 16S rRNA sequencing.ResultsCompared with the HC and ON groups, patients with EN exhibited significantly different compositions of the intestinal microbiota in the first week, including enrichment of the opportunistic pathogen Enterococcus and depletion of bacteria such as Lachnospiraceae, and Ruminococcus, which were further depleted in the second week. An increase in Parvimonas and Comamonas abundances was associated with an increased risk of 180‐day mortality.ConclusionsThe intestinal microbiota in ischemic stroke patients receiving EN is significantly altered, and specific strains of bacteria may be associated with prognosis and clinical indicators.
Inhibition of horseradish peroxidase activity through conformational change in surfactant solution
Authors: Wang Jiuxia , Yao Meihuan , Bai Guangyue , Liu Junling , Wang Yujie 
Published: March 2022

AbstractSurfactants have a considerable potential for inhibiting horseradish peroxidase (HRP) activity; yet, more research is needed to understand the inhibition mechanism to allow for choosing the suitable surfactant candidate as inhibitor. In this study, three traditional surfactants, sodium dodecyl sulfate (SDS), N‐dodecyltrimethyl ammonium bromide (DTAB) and N‐dodecyldimethyl (3‐sulfopropyl) ammonium hydroxide (SB3‐12), that share the same (C12) hydrophobic tail but possess different charged head groups, were taken as model surfactant inhibitors, and the enzymatic activity of HRP was assessed in these surfactant solutions. The activity of HRP was inhibited by both anionic SDS and cationic DTAB at dilute concentration even below their critical micelle concentration (cmc), and the electrostatic interaction of the ionic surfactants with some amino residues of HRP was mainly responsible for the inhibition of activity. HRP in a dilute solution of SB3‐12, with the latter's concentration being below its cmc, retained a high level of enzymatic activity; but, at concentrations above the cmc of SB3‐12, HRP was inhibited owing to the synergetic interaction of the SB3‐12 micelle with HRP. Results from circular dichroism (CD) and intrinsic fluorescence spectroscopic analyses of HRP showed the unfolding of the tertiary structure around HRP's heme active site played a critical role in the inhibition, whereas the changes of HRP in the secondary structure and the tertiary structure near its tryptophan residue (Trp117) induced by these surfactants were minor for the inhibition. Based on the experimental results, a relationship between conformation and activity for HRP was suggested.
Effect of biotic stress on the presence of secondary metabolites in field pea grains
Authors: Oliete Bonastre , Lubbers Samuel , Fournier Carine , Jeandroz Sylvain , Saurel Rémi 
Published: March 2022

AbstractBACKGROUNDThe presence of secondary metabolites responsible for off‐flavours in peas may influence consumers' acceptance. These undesirable compounds may increase due to biotic stress or cultivar. Therefore, grains from two pea (Pisum sativum L.) cultivars (Crécerelle and Firenza) exposed to biotic stress were studied in terms of protein content, electrophoretic polypeptide profile, lipoxygenase activity, saponin content and volatile compounds.RESULTSNo differences were observed in the electrophoretic polypeptide profile of pea samples across cultivar or biotic stress. The cultivar noticeably affected the volatile compounds and lipoxygenase activity. The biotic stress significantly increased the saponin content.CONCLUSIONThe cultivar showed more noticeable impact on the presence of off‐flavour compounds than the biotic stress. The development of pea protein ingredients needs the thorough choice of raw materials in terms of cultivar and control of biotic stress in order to ensure acceptance by consumers. © 2022 Society of Chemical Industry.
Encapsulation of curcumin in soluble soybean polysaccharide‐coated gliadin nanoparticles: interaction, stability, antioxidant capacity, and bioaccessibility
Authors: Guo Siyan , Zhao Yanyan , Luo Shuizhong , Mu Dongdong , Li Xingjiang , Zhong Xiyang , Jiang Shaotong , Zheng Zhi 
Published: March 2022

AbstractBACKGROUNDGliadin nanoparticles are used as a delivery system for active substances because of their amphiphilicity and bioavailability. However, they are susceptible to destabilization by external agents. In this study, gliadin nanoparticles stabilized by soluble soybean polysaccharide (SSPS) were prepared by antisolvent precipitation. Formed stable complex nanoparticles were applied to protect and deliver curcumin (Cur).RESULTSGliadin/SSPS nanoparticles with the smallest particle size (196.66 nm, polydispersity index < 0.2) were fabricated when the mass ratio of gliadin to SSPS was 1:1 at pH 5.0. SSPS‐stabilized gliadin nanoparticles had excellent stability at pH 3.0–8.0, 0.02–0.1 mol L−1 NaCl and at 90 °C heat. Gliadin/SSPS nanoparticles were used to encapsulate the Cur. The encapsulation efficiency of the Cur‐loaded gliadin/SSPS nanoparticles was 84.59%. Fourier transform infrared spectroscopy and fluorescence spectrophotometry showed that the main forces were hydrogen bonds, electrostatic interactions and hydrophobic interactions between gliadin and SSPS. The X‐ray diffraction patterns exhibited that the crystalline form of Cur converted to an amorphous substance. The retention rates of Cur‐loaded gliadin/SSPS nanoparticles reached 79.03%, 73.43% and 87.92% after ultraviolet irradiation for 4 h, heating at 90 °C and storage at 25 °C for 15 days, respectively. Additionally, simulated digestion demonstrated that the bioavailability of gliadin/SSPS‐Cur nanoparticles was four times higher than that of free Cur.CONCLUSIONThis study showed that SSPS improved the stability of gliadin nanoparticles. Gliadin/SSPS nanoparticles have the function of loading and delivering Cur. © 2022 Society of Chemical Industry