bluuebunny/crossref_metadata_2025_split huggingface dataset:
A cost-utility analysis between decompression only and fusion surgery for elderly patients with lumbar spinal stenosis and sagittal imbalance
Authors: Won Young Il , Kim Chi Heon , Park Hee-Pyoung , Chung Sun Gun , Yuh Woon Tak , Kwon Shin Won , Yang Seung Heon , Lee Chang-Hyun , Choi Yunhee , Park Sung Bae , Rhee John M. , Kim Kyoung-Tae , Chung Chun Kee 
Published: November 2022

AbstractLumbar spinal stenosis (LSS) and sagittal imbalance are relatively common in elderly patients. Although the goals of surgery include both functional and radiological improvements, the criteria of correction may be too strict for elderly patients. If the main symptom of patients is not forward-stooping but neurogenic claudication or pain, lumbar decompression without adding fusion procedure may be a surgical option. We performed cost-utility analysis between lumbar decompression and lumbar fusion surgery for those patients. Elderly patients (age > 60 years) who underwent 1–2 levels lumbar fusion surgery (F-group, n = 31) or decompression surgery (D-group, n = 40) for LSS with sagittal imbalance (C7 sagittal vertical axis, C7-SVA > 40 mm) with follow-up ≥ 2 years were included. Clinical outcomes (Euro-Quality of Life-5 Dimensions, EQ-5D; Oswestry Disability Index, ODI; numerical rating score of pain on the back and leg, NRS-B and NRS-L) and radiological parameters (C7-SVA; lumbar lordosis, LL; the difference between pelvic incidence and lumbar lordosis, PI-LL; pelvic tilt, PT) were assessed. The quality-adjusted life year (QALY) and incremental cost-effective ratio (ICER) were calculated from a utility score of EQ-5D. Postoperatively, both groups attained clinical and radiological improvement in all parameters, but NRS-L was more improved in the F-group (p = 0.048). ICER of F-group over D-group was 49,833 US dollars/QALY. Cost-effective lumbar decompression may be a recommendable surgical option for certain elderly patients, despite less improvement of leg pain than with fusion surgery.
Multiturn dialogue generation by modeling sentence-level and discourse-level contexts
Authors: Yang Yang , Cao Juan , Wen Yujun , Zhang Pengzhou 
Published: November 2022

AbstractCurrently, multiturn dialogue models generate human-like responses based on pretrained language models given a dialogue history. However, most existing models simply concatenate dialogue histories, which makes it difficult to maintain a high degree of consistency throughout the generated text. We speculate that this is because the encoder ignores information about the hierarchical structure between sentences. In this paper, we propose a novel multiturn dialogue generation model that captures contextual information at the sentence level and at the discourse level during the encoding process. The context semantic information is dynamically modeled through a difference-aware module. A sentence order prediction training task is also designed to learn representation by reconstructing the order of disrupted sentences with a learning-to-rank algorithm. Experiments on the multiturn dialogue dataset, DailyDialog, demonstrate that our model substantially outperforms the baseline model in terms of both automatic and human evaluation metrics, generating more fluent and informative responses than the baseline model.
COVID-19-related stress, exercise, and oral health-related quality of life among community-dwelling older adults who participated in the CHEER Iwamizawa project, Japan
Authors: Miura Kazuhito , Watanabe Yutaka , Baba Haruhisa , Ozaki Kimiya , Matsushita Takae , Kondoh Miyako , Okada Kazutaka , Nakaoka Shinji , Ogasawara Katsuhiko , Suzuki Teppei , Saito Hiroshi , Kimura Takashi , Tamakoshi Akiko , Yamazaki Yutaka 
Published: November 2022

AbstractThis study examined the association between coronavirus disease 2019 (COVID-19)-related stress, exercise habits, and oral health-related quality of life (OHRQoL) in a sample of 215 community-dwelling older adults in Japan (57 men, 158 women; Mage = 74.2 years, SD = 6.0). Data were collected during wellness checkups in October 2020 and included participants’ demographic characteristics, measures of instrumental activities of daily living and depressive tendencies, number of teeth, oral hypofunction, OHRQoL, COVID-19-related stress, and exercise habits. Four mutually exclusive groups were created, using the presence or absence of COVID-19-related stress and lack of exercise habits as risk factors for poor OHRQoL (no COVID-19-related stress and no lack of exercise, COVID-19-related stress only, lack of exercise habits only, and both COVID-19-related stress and lack of exercise habits). Poisson regression with robust standard errors provided the prevalence ratio for poor OHRQoL. The presence of both COVID-19-related stress and lack of exercise habits (adjusted prevalence ratio: 2.20, 95% CI: 1.31– 3.69) was associated with poor OHRQoL. The results indicate that COVID-19-related stress and exercise habits should be considered when designing oral health and public health initiatives.
Necessity of integrated genomic analysis to establish a designed knock-in mouse from CRISPR-Cas9-induced mutants
Authors: Yoshida Masahide , Saito Tomoko , Takayanagi Yuki , Totsuka Yoshikazu , Onaka Tatsushi 
Published: November 2022

AbstractThe CRISPR-Cas9 method for generation of knock-in mutations in rodent embryos yields many F0 generation candidates that may have the designed mutations. The first task for selection of promising F0 generations is to analyze genomic DNA which likely contains a mixture of designed and unexpected mutations. In our study, while generating Prlhr-Venus knock-in reporter mice, we found that genomic rearrangements near the targeted knock-in allele, tandem multicopies at a target allele locus, and mosaic genotypes for two different knock-in alleles occurred in addition to the designed knock-in mutation in the F0 generation. Conventional PCR and genomic sequencing were not able to detect mosaicism nor discriminate between the designed one-copy knock-in mutant and a multicopy-inserted mutant. However, by using a combination of Southern blotting and the next-generation sequencing-based RAISING method, these mutants were successfully detected in the F0 generation. In the F1 and F2 generations, droplet digital PCR assisted in establishing the strain, although a multicopy was falsely detected as one copy by analysis of the F0 generation. Thus, the combination of these methods allowed us to select promising F0 generations and facilitated establishment of the designed strain. We emphasize that focusing only on positive evidence of knock-in can lead to erroneous selection of undesirable strains.
Dual-functional communication and sensing antenna system
Authors: Alam Tanjir , Cheffena Michael , Rajo-Iglesias Eva 
Published: November 2022

AbstractIn this article, a dual functional antenna system for communication and sensing applications is designed on a single substrate having a common input port. The main motivation behind the proposed design is to build an antenna system, which can work as an antenna sensor and also can communicate over a fixed band not related to the resonance frequency shifts of the antenna sensor. The dual functionality of the system has been achieved by designing a frequency selective multipath filter (FSMF). The FSMF has an input port and two output ports. Both the communicating and sensing antennas are integrated on the output ports of the FSMF. The FSMF ensures minimum effect of the antennas on each other’s performance. The performance of the antenna sensor is first shown by characterizing different standard substrates with $${\varepsilon} _r=2.2$$

ε
r

=
2.2

to 6.15 and then demonstrated for ice and water detection. The communicating antenna is used for Wi-Fi (2.45 GHz) applications. The simulated and measured results of the dual-functional antenna systems are in good agreement. The proposed design is fabricated on a PCB with $${\varepsilon} _r=3.5$$

ε
r

=
3.5

and $$\tan \delta =0.0027$$

tan
δ
=
0.0027

with an overall size of $$86.7\times 26\times 0.8\,\text {mm}^3$$

86.7
×
26
×
0.8

mm
3

.
Structural equation model based on salutogenesis theory for evaluating factors affecting health-related quality of life in adolescents with moyamoya disease
Authors: Oh Won-oak , Yeom Insun , Lim Sung-Hyun 
Published: November 2022

AbstractMoyamoya disease is a cerebrovascular disorder and a significant chronic health concern requiring regular monitoring to control the disease and its related complications. We examined a hypothetical model by integrating the concepts of a structural health-related quality-of-life model based on the salutogenesis theory, and to identify how social support, sense of coherence, and stress contribute to health behaviors, subjective health status, and quality of life in adolescents with moyamoya disease among 239 adolescents in Korea. A structural equation model was used to analyze the data. The fitness of the hypothetical model with the salutogenesis theory was satisfactory, showing that the goodness-of-fit index = 0.91, adjusted goodness-to-fit index = 0.90, comparative fit index = 0.92, normed fit index = 0.91, incremental fit index = 0.91, standardized root mean squared residual = 0.04, root mean square error of approximation = 0.07, parsimony normed fit index = 0.61, parsimony goodness of fit index = 0.51. The model explained 68.9% of quality of life. Health behavior (β = −0.173, p = 0.467) and stress (β = −0.557, p < 0.001) had significant direct and total effects on quality of life. Sense of coherence had a significant direct (β = 0.371, p = 0.003), indirect (β = 0.220, p = 0.013), and total (β = 0.590, p < 0.001) effect on quality of life. This study found that sense of coherence was significant factors contributing to lower stress, improved health status, and quality of life in adolescents with moyamoya disease. To improve the quality of life for adolescents with moyamoya disease, comprehensive nursing interventions need to be developed and applied.
Obsessive–compulsive symptoms are negatively correlated with motor severity in patients with generalized dystonia
Authors: Matsuda Taku , Morigaki Ryoma , Matsumoto Yuki , Mure Hideo , Miyake Kazuhisa , Nakataki Masahito , Harada Masafumi , Takagi Yasushi 
Published: November 2022

AbstractWe aimed to clarify the correlations between motor symptoms and obsessive–compulsive symptoms and between the volumes of basal ganglia components and obsessive–compulsive symptoms. We retrospectively included 14 patients with medically intractable, moderate and severe generalized dystonia. The Burke–Fahn–Marsden Dystonia Rating Scale and Maudsley Obsessional Compulsive Inventory were used to evaluate the severity of dystonia and obsessive–compulsive symptoms, respectively. Patients with generalized dystonia were divided into two groups; patients whose Maudsley Obsessional Compulsive Inventory score was lower than 13 (Group 1) and 13 or more (Group 2). Additionally, the total Maudsley Obsessional Compulsive Inventory scores in patients with dystonia were significantly higher than normal volunteers’ scores (p = 0.025). Unexpectedly, Group 2 (high Maudsley Obsessional Compulsive Inventory scores) showed milder motor symptoms than Group 1 (low Maudsley Obsessional Compulsive Inventory scores) (p = 0.016). “Checking” rituals had a strong and significant negative correlation with the Burke–Fahn–Marsden Dystonia Rating Scale (ρ = − 0.71, p = 0.024) and a strong positive correlation with the volumes of both sides of the nucleus accumbens (right: ρ = 0.72, p = 0.023; left: ρ = 0.70, p = 0.034). Our results may provide insights into the pathogenesis of obsessive–compulsive disorder and dystonia.
Spattering mechanism of laser powder bed fusion additive manufacturing on heterogeneous surfaces
Authors: Ikeshoji Toshi-Taka , Yonehara Makiko , Kato Chika , Yanaga Yuma , Takeshita Koki , Kyogoku Hideki 
Published: November 2022

AbstractLaser powder additive manufacturing (PBF-LB) is an additive manufacturing method capable of producing high-precision and fully dense parts. However, nondestructively quality assurance of no internal defects remains challenging. Mitigating internal defects requires elucidating their formation mechanism and improving the PBF-LB process conditions. Therefore, we developed an in-situ monitoring system that combines surface morphology measurement by fringe projection and thermal field measurement with a high-speed camera. On heterogeneous surfaces in a practical multi-track PBF-LB process, a roughness index of the built part surface altered cyclically, consistent with the change in the angle between laser scanning and atmospheric gas flow. The high-speed camera monitoring showed that the melt pool was asymmetrical and spindle-shaped and that spatter was emitted mainly from the built part side of the melt pool. Furthermore, it was found that the built-part surface morphology under the powder layer affected the stability of the melt pool. As a result, a graphical representation of the melt pool and spattering for heterogeneous surfaces was proposed. Although it is still difficult to theoretically estimate the process window in which no spattering and no internal defects, in-situ monitoring equipment will provide knowledge to elucidate spattering and internal defects formation.
Apoptotic-like PCD inducing HRC gene when silenced enhances multiple disease resistance in plants
Authors: Kushalappa A. C. , Hegde N. G. , Gunnaiah R. , Sathe A. , Yogendra K. N. , Ajjamada L. 
Published: November 2022

AbstractProgrammed cell death (PCD) plays an important role in plant environmental stress and has the potential to be manipulated to enhance disease resistance. Plants have innate immunity and, following pathogen perception, the host induces a Hypersensitive Response PCD (HR-PCD), leading to pattern (PTI) or effector triggered immunity (ETI). Here we report a non-HR type or Apoptotic-Like PCD (AL-PCD) in pathogen infected wheat and potato based on apoptotic-like DNA fragmentation. A deletion mutation in the gene encoding histidine rich calcium binding protein (TaHRC) in FHB-resistant wheat (R-NIL) failed to induce AL-PCD. Similarly, the CRISPR-Cas9 based silencing ofStHRCgene in Russet Burbank potato failed to induce apoptotic-like DNA fragmentation, proved based on DNA laddering and TUNEL assays. The absence of AL-PCD in wheat R-NIL reduced pathogen biomass and mycotoxins, increasing the accumulation of resistance metabolites and FHB-resistance, and in potato it enhanced resistance to multiple pathogens. In addition, the reduced expressions of metacaspase (StMC7) and Ca2+dependent endonuclease 2 (StCaN2) genes in potato withSthrcindicated an involvement of a hierarchy of genes in the induction of AL-PCD. TheHRCin commercial varieties of different crops, if functional, can be silenced by genome editing possibly to enhance resistance to multiple pathogens.
Van der Waals interactions regulating the hydration of 2-methacryloyloxyethyl phosphorylcholine, the constructing monomer of biocompatible polymers
Authors: Takahashi Masae , Chen Sifan , Matsui Hiroshi , Morimoto Nobuyuki , Ikemoto Yuka 
Published: November 2022

AbstractVan der Waals (VDW) interactions provide fantastic properties for biological systems that function at room temperature. The VDW interaction, which primarily contributes to weak hydrogen bonding, is expected to play a key role in regulating hydrophobic hydration to express the biologically inert biocompatible function of polymerized MPCs (2-methacryloyloxyethyl phosphorylcholine). This report explores at the molecular level the biologically inert function of polymerized MPCs through an array of vibrational spectroscopic and computational characterization of MPC monomers, as temperature-dependent change of intramolecular weak hydrogen bonding. Synchrotron Fourier transform infrared microspectroscopy and terahertz time-domain spectroscopy were used to investigate temperature-dependent spectral changes in the low frequency vibrations of the MPC over the temperature range from cryogenic to room temperature, and the results were analysed by highly reliable well-established density functional theory (DFT) calculations. Complicated spectral features in the low frequency energy region and the uncertain conformations of the MPC in the amorphous powder state are clearly resolved under a polarizable continuum model and dispersion correction to pure DFT calculations.
Label-free multimodal imaging of infected Galleria mellonella larvae
Authors: Quansah Elsie , Ramoji Anuradha , Thieme Lara , Mirza Kamran , Goering Bianca , Makarewicz Oliwia , Heutelbeck Astrid , Meyer-Zedler Tobias , Pletz Mathias W. , Schmitt Michael , Popp Jürgen 
Published: November 2022

AbstractNon-linear imaging modalities have enabled us to obtain unique morpho-chemical insights into the tissue architecture of various biological model organisms in a label-free manner. However, these imaging techniques have so far not been applied to analyze the Galleria mellonella infection model. This study utilizes for the first time the strength of multimodal imaging techniques to explore infection-related changes in the Galleria mellonella larvae due to massive E. faecalis bacterial infection. Multimodal imaging techniques such as fluorescent lifetime imaging (FLIM), coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence (TPEF), and second harmonic generation (SHG) were implemented in conjunction with histological HE images to analyze infection-associated tissue damage. The changes in the larvae in response to the infection, such as melanization, vacuolization, nodule formation, and hemocyte infiltration as a defense mechanism of insects against microbial pathogens, were visualized after Enterococcus faecalis was administered. Furthermore, multimodal imaging served for the analysis of implant-associated biofilm infections by visualizing biofilm adherence on medical stainless steel and ePTFE implants within the larvae. Our results suggest that infection-related changes as well as the integrity of the tissue of G. mellonella larvae can be studied with high morphological and chemical contrast in a label-free manner.
Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts
Authors: McLaughlin Autumn A. , Hanley-Bowdoin Linda , Kennedy George G. , Jacobson Alana L. 
Published: November 2022

AbstractThis study investigated the role of vector acquisition and transmission on the propagation of single and co-infections of tomato yellow leaf curl virus (TYLCV,) and tomato mottle virus (ToMoV) (Family: Geminiviridae, Genus: Begomovirus) by the whitefly vector Bemisia tabaci MEAM1 (Gennadius) in tomato. The aim of this research was to determine if the manner in which viruses are co-acquired and co-transmitted changes the probability of acquisition, transmission and new host infections. Whiteflies acquired virus by feeding on singly infected plants, co-infected plants, or by sequential feeding on singly infected plants. Viral titers were also quantified by qPCR in vector cohorts, in artificial diet, and plants after exposure to viruliferous vectors. Differences in transmission, infection status of plants, and titers of TYLCV and ToMoV were observed among treatments. All vector cohorts acquired both viruses, but co-acquisition/co-inoculation generally reduced transmission of both viruses as single and mixed infections. Co-inoculation of viruses by the vector also altered virus accumulation in plants regardless of whether one or both viruses were propagated in new hosts. These findings highlight the complex nature of vector-virus-plant interactions that influence the spread and replication of viruses as single and co-infections.
Expression of HLA class I is associated with immune cell infiltration and patient outcome in breast cancer
Authors: Han Song-Hee , Kim Milim , Chung Yul Ri , Woo Ji Won , Choi Hye Yeon , Park So Yeon 
Published: November 2022

AbstractDownregulation of human leukocyte antigen (HLA) class I is one mechanism of escaping anti-tumor immunity by tumor cells. This study was conducted to compare HLA class I expression in ductal carcinoma in situ (DCIS) and invasive breast carcinoma (IBC) and to evaluate its association with immune cell infiltration of the tumors and clinical outcome of the patients. A total of 830 cases comprising 288 DCIS and 542 IBC were included in this study. Immunohistochemistry for HLA class I expression was performed using HLA-ABC in tissue microarrays and was analyzed in relation to clinicopathologic characteristics of tumors and infiltration of CD4+, CD8+, and FOXP3+ tumor-infiltrating lymphocyte (TIL) subsets and PD-L1+ immune cells. As a whole, there was no difference in HLA class I expression between DCIS and IBC when dichotomized into high or low expression. However, in the HR-negative group, a high level of HLA class I expression was more frequent in IBC than DCIS. On the contrary, in the HR-positive group, a complete loss of HLA class I expression was more frequently observed in IBC than DCIS. High HLA class I expression level was generally associated with aggressive clinicopathologic features of IBC and was associated with high CD4+, CD8+, and FOXP3+ TIL and PD-L1+ immune cell infiltration in both DCIS and IBC. In survival analyses, HLA class I expression was not associated with clinical outcome in DCIS and IBC as a whole; however, low HLA class I expression was associated with poor clinical outcome in HR-negative IBC, especially in triple-negative subtype. In conclusion, this study showed that HLA class I expression increased in association with increased immune cell infiltration during in situ to invasive transition of HR-negative breast cancer, and HLA class I down-regulation had a prognostic value in HR-negative breast cancer.