bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growth factors‐based platelet lysate rejuvenates skin against ageing through NF‐κB signalling pathway: In vitro and in vivo mechanistic and clinical studies
Authors: Li Ting , Lu Haishan , Zhou Li , Jia Ming , Zhang Lei , Wu Huiling , Shan Letian 
Published: March 2022

AbstractIntroductionPlatelets benefit tissue regeneration by secreting growth factors, and platelet products, for example, platelet lysate (PL), have been clinically applied for tissue rejuvenation. To determine the anti‐ageing efficacy and mechanism of human PL (hPL) on skin, this study conducted clinical retrospective analysis, nude mice‐based in vivo study and human dermal fibroblasts (HDFs)‐based in vitro study.MethodsFlow cytometry was employed for quality control of hPL, and ELISA was used for quantification of growth factors (EGF, IGF‐1, PDGF and TGF‐β) in hPL. After d‐galactose modelling, skin texture grading, histopathological observation, immunofluorescence analysis and oxidative stress assays were conducted on nude mice, while SA‐β‐gal staining, CCK‐8 and wound healing assays were conducted on HDFs. qPCR and western blot were conducted to clarify hPL's mechanism.ResultsThe clinical retrospective data showed that hPL obviously rejuvenated human skin appearances without adverse events. The animal data showed that hPL exerted rejuvenative effects on skin, and the cellular data showed that hPL significantly promoted the proliferation and migration of HDFs and suppressed senescence‐associated secretory protein secretion and senescence state of senescent HDFs by suppressing NF‐κB pathway. The NF‐κB‐dependent mechanism was verified positively by using P65 siRNA and negatively by using prostratin. Furthermore, EGF, IGF‐1, PDGF and TGF‐β were found as the main ingredients in hPL, which contributed to the efficacy and mechanism of hPL.ConclusionThis study provided novel knowledge of hPL, making it ideal for skin rejuvenation.
In situ analysis of acupuncture protecting dopaminergic neurons from lipid peroxidative damage in mice of Parkinson's disease
Authors: Zuo Tingting , Xie Mo , Yan Meiling , Zhang Zengyan , Tian Tian , Zhu Ying , Wang Lihua , Sun Yanhong 
Published: March 2022

AbstractObjectivesAcupuncture stimulation has proven to protect dopaminergic neurons from oxidative damage in animal models of Parkinson's disease (PD), but it remains unclear about the in situ information of biochemical components in dopaminergic neurons. Here, we aimed to analyse in situ changes of biochemical components and lipid peroxidation levels in dopaminergic neurons in PD mice treated with acupuncture by synchrotron FTIR micro‐spectroscopy technique.Materials and MethodsAbout 9–10‐week‐old C57BL/6 mice were used to establish PD model by intraperitoneal injection of 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP, 30 mg/kg for 5 days). Acupuncture stimulation was performed once a day for 12 days. Behaviour test was determined using the rotarod instrument. Biochemical compositions of dopaminergic neurons in substantia nigra pars compacta were analysed by synchrotron FTIR micro‐spectroscopy technique. The number and ultrastructure of dopaminergic neurons were respectively observed by immunofluorescence and transmission electron microscopy (TEM).ResultsWe found that the number and protein expression of dopaminergic neurons in MPTP‐treated mice were reduced by about half, while that in the mice treated by acupuncture were significantly restored. Acupuncture treatment also restored the motor ability of PD mice. The results of single cell imaging with synchrotron FTIR micro‐spectroscopy technique showed that the proportion of lipid in MPTP treated mice increased significantly. Especially the ratio of CH2 asymmetric stretching and CH3 asymmetric stretching increased significantly, suggesting that MPTP induced lipid peroxidation damage of dopaminergic neurons. It is also supported by the result of TEM, such as mitochondrial swelling or atrophy, loss of mitochondrial crests and mitochondrial vacuolization. Compared with MPTP treated mice, the proportion of lipid in acupuncture treated mice decreased and the mitochondrial structure was restored.ConclusionsAcupuncture can inhibit the level of lipid peroxides in dopaminergic neurons and protect neurons from oxidative damage. The study provides a promising method for in situ analysis of biochemical compositions in PD mice and reveals the mechanism of acupuncture in treating neurodegenerative diseases.
Peritumoral matrilin‐2 staining may be useful in distinguishing basal cell carcinoma from folliculocentric basaloid proliferation
Authors: Wang Zhengke , Lee Jennifer , Breen Catherine , Ruenger Thomas , Xiong Fang , Goreshi Renato , Lu Ming , Iwamoto Satori 
Published: March 2022

AbstractBackgroundFolliculocentric basaloid proliferation (FBP) is a benign and reactive proliferation which can histopathologically mimic basal cell carcinomas (BCCs). The incidental presence of FBP during the excision of a BCC can occasionally lead to excessive tissue removal. One distinguishing feature of BCCs is that they invade the stroma, whereas FBPs generally do not.MethodsMatrilin‐2 is an extracellular matrix protein associated with tumor invasion, and we compared the expression of matrilin‐2 in peritumoral cells of BCC and FBP.ResultsWe found increased matrilin‐2 expression within the peritumoral stroma of 41 of 42 BCCs (97.7%), with strong expression in all (100%) cases of infiltrative subtypes and in 21 of 25 (84%) nodular subtypes of BCC. We found no expression of peritumoral matrilin‐2 in any of the seven cases of FBP.ConclusionOur results suggest that immunolabeling with the matrilin‐2 antibody may help distinguish BCCs from FBPs.
Functional diversity of epigeal spiders in the olive grove agroecosystem in northeastern Portugal: a comparison between crop and surrounding semi‐natural habitats
Authors: Kubiak Ketrin Lorhayne , Pereira José Alberto , Tessaro Dinéia , Santos Sónia A.P. , Benhadi‐Marín Jacinto 
Published: March 2022

AbstractThe olive grove agroecosystem (Olea europaea L., Oleaceae) is very important in terms of economy and culture throughout the Mediterranean region. In Trás‐os‐Montes (Portugal), olive groves are commonly surrounded by semi‐natural areas consisting of Mediterranean shrublands. Understanding the role of potential predators of olive pests is crucial to enhancing biological control in sustainable agriculture. This study aimed to investigate the community structure of ground‐inhabiting spiders (Arachnida: Araneae) in the olive grove and their surrounding shrublands in Trás‐os‐Montes. We found that the community of spiders was dominated by ground hunters such as Lycosidae and Gnaphosidae. Lycosidae were more abundant in olive groves than in surrounding shrublands in 2015 only, whereas Gnaphosidae were more abundant in olive groves in both years of the study (2015 and 2016). Hence, the two habitats displayed a low complementarity of spider families. The Mediterranean shrubland areas could act as a reservoir and refuge for potential pest biocontrol agents. Conservation of surrounding semi‐natural areas may contribute to the natural limitation of pests exerted by spiders in the olive grove agroecosystem. Nevertheless, further long‐time and species‐level studies are needed to provide insights into the role of spiders as natural enemies.
Wealth inequality in pre‐industrial England: A long‐term view (late thirteenth to sixteenth centuries)
Authors: Alfani Guido , García Montero Hector 
Published: March 2022

AbstractThis article provides an overview of wealth inequality in England from the late thirteenth to the sixteenth century, based on a novel database of distributions of taxable household wealth across 17 counties plus London. To account for high thresholds of fiscal exemption, a new method is introduced to reconstruct complete distributions from left‐censored observations. First, we analyse inequality at the county level, finding an impressive stability across time in the relative position of the English counties, perturbed only by the tendency of the South and South‐East to become relatively more inegalitarian. Then, we produce an aggregate distribution representative of England as a whole, and we detect an overall tendency for inequality to grow from medieval to early modern times due largely to North–South divergence in average household wealth. We discuss our results in the light of the recent literature on historical inequality.
Survival in autoimmune hemolytic anemia remains poor, results from a nationwide cohort with 37 years of follow‐up
Authors: Hansen Dennis Lund , Möller Sören , Frederiksen Henrik 
Published: March 2022

AbstractIntroductionAutoimmune hemolytic anemia (AIHA) is considered a chronic disease, with an overall good prognosis. However, recent reports indicate pre‐mature mortality. Causes of death have not been evaluated previously.MethodsIn a nationwide setting, we identified all patients with warm type AIHA or cold agglutinin disease (CAD), and age–sex‐matched comparators from Denmark, 1980–2016. We estimated overall survival and cause‐specific mortality from anemia, infection, cardiovascular causes, hematological or solid cancer, bleeding, or other causes, using cumulative incidence proportions.ResultsWe identified 1460 patients with primary AIHA, 1078 with secondary AIHA, 112 with CAD, and 130 801 comparators. One‐year survival and median survival were, 82.7% and 9.8 years for primary AIHA, 69.1% and 3.3 years for secondary AIHA, and 85.5% and 8.8 years for CAD. Prognosis was comparable to the general population only in patients with primary AIHA below 30 years. In all other age and subgroups, the difference was considerable. Cumulated cause‐specific mortality at 1 year was increased among patients versus comparators.DiscussionAll groups of autoimmune hemolytic anemia are associated with increased overall and cause‐specific mortality compared to the general population. This probably reflects unmet needs in both treatment and follow‐up programs.
Highly expressed genes in multiple myeloma cells – what can they tell us about the disease?
Authors: Børset Magne , Elsaadi Samah , Vandsemb Esten N. , Hess Eli Svorkdal , Steiro Ida J. , Cocera Fernandez Miguel , Sponaas Anne‐Marit , Abdollahi Pegah 
Published: March 2022

AbstractCancer cells can convert proto‐oncoproteins into oncoproteins by increasing the expression of genes that are oncogenic when expressed at high levels. Such genes can promote oncogenesis without being mutated. To find overexpressed genes in cancer cells from patients with multiple myeloma, we retrieved mRNA expression data from the CoMMpass database and ranked genes by their expression levels. We grouped the most highly expressed genes based on a set of criteria and we discuss the role a selection of them can play in the disease pathophysiology. The list was highly concordant with a similar list based on mRNA expression data from the PADIMAC study. Many well‐known “myeloma genes” such as MCL1, CXCR4, TNFRSF17, SDC1, SLAMF7, PTP4A3, and XBP1 were identified as highly expressed, and we believe that hitherto unrecognized key players in myeloma pathogenesis are also enriched on the list. Highly expressed genes in malignant plasma cells that were absent or expressed at only a low level in healthy plasma cells included IFI6, IFITM1, PTP4A3, SIK1, ALDOA, ATP5MF, ATP5ME, and PSMB4. The ambition of this article is not to validate the role of each gene but to serve as a guide for studies aiming at identifying promising treatment targets.
Midsagittal corpus callosal thickness and cognitive impairment in Parkinson's disease
Authors: Owens‐Walton Conor , Adamson Chris , Walterfang Mark , Hall Sara , van Westen Danielle , Hansson Oskar , Shaw Marnie , Looi Jeffrey C. L. 
Published: March 2022

AbstractPeople diagnosed with Parkinson's disease (PD) can experience significant neuropsychiatric symptoms, including cognitive impairment and dementia, the neuroanatomical substrates of which are not fully characterised. Symptoms associated with cognitive impairment and dementia in PD may relate to direct structural changes to the corpus callosum via primary white matter pathology or as a secondary outcome due to the degeneration of cortical regions. Using magnetic resonance imaging, the corpus callosum can be investigated at the midsagittal plane, where it converges to a contiguous mass and is not intertwined with other tracts. The objective of this project was thus twofold: First, we investigated possible changes in the thickness of the midsagittal callosum and cortex in patients with PD with varying levels of cognitive impairment; and secondly, we investigated the relationship between the thickness of the midsagittal corpus callosum and the thickness of the cortex. Study participants included cognitively unimpaired PD participants (n = 35), PD participants with mild cognitive impairment (n = 22), PD participants with dementia (n = 17) and healthy controls (n = 27). We found thinning of the callosum in PD‐related dementia compared with PD‐related mild cognitive impairment and cognitively unimpaired PD participants. Regression analyses found thickness of the left medial orbitofrontal cortex to be positively correlated with thickness of the anterior callosum in PD‐related mild cognitive impairment. This study suggests that a midsagittal thickness model can uncover changes to the corpus callosum in PD‐related dementia, which occur in line with changes to the cortex in this advanced disease stage.
Determinants of disability at 6 months after stroke: The GRECogVASC Study
Authors: Tasseel‐Ponche Sophie , Barbay Mélanie , Roussel Martine , Lamrani Adnane , Sader Thibaud , Arnoux‐Courselle Audrey , Canaple Sandrine , Lamy Chantal , Leclercq Claire , Aarabi Ardalan , Schnitzler Alexis , Yelnik Alain Pierre , Godefroy Olivier ,   GRECogVASC Study Group
Published: March 2022

AbstractBackground and purposeThe aim of this study was to determine the contributions of background disorders responsible for participation restriction as indexed by a structured interview for the modified Rankin Scale (mRS‐SI).MethodsA subset of 256 patients was assessed at 6 months after stroke using the National Institutes of Health Stroke Scale (NIHSS), gait score, comprehensive cognitive battery (yielding a global cognitive Z‐score), behavioral dysexecutive disorders (DDs), anxiety and depressive symptoms, epilepsy, and headache. Following bivariate analyses, determinants of participation restriction were selected using ordinal regression analysis with partial odds.ResultsPoststroke participation restriction (mRS‐SI score > 1) was observed in 59% of the patients. In bivariate analyses, mRS‐SI score was associated with prestroke mRS‐SI score, 6‐month NIHSS score, gait score, global cognitive Z‐score, behavioral DDs, and presence of anxiety and depression (all: p = 0.0001; epilepsy: p =0.3; headache: p = 0.7). After logistic regression analysis, NIHSS score was associated with increasing mRS‐SI score (p = 0.00001). Prestroke mRS‐SI score (p = 0.00001), behavioral DDs (p = 0.0008) and global cognitive Z‐score (p = 0.01) were associated with both mRS‐SI score > 1 and mRS‐SI score > 2. In addition, gait score was associated with mRS‐SI score > 2 (p = 0.00001). This model classified 85% of mRS‐SI scores correctly (p = 0.001). Structural equation modeling showed the contributions of gait limitation (standardized coefficient [SC]: 0.68; p = 0.01), prestroke mRS‐SI (SC: 0.41; p = 0.01), severity of neurological impairment (SC: 0.16; p = 0.01), global cognitive Z‐score (SC: −0.14; p = 0.05), and behavioral DDs (SC: 0.13; p = 0.01).ConclusionThese results provide a statistical model of weights of determinants responsible for poststroke participation restriction and highlight a new independent determinant: behavioral DDs.
Seasonal variation in the response to a toxin‐producing cyanobacteria in Daphnia
Authors: Hegg Alexander , Radersma Reinder , Uller Tobias 
Published: March 2022

Abstract

Many populations of water fleas (Daphnia) are exposed to algal blooms dominated by microcystin‐producing cyanobacteria. However, the severity of these effects on Daphnia fitness remain poorly understood in natural populations.

We investigated seasonal changes in body size, reproduction and survival of D. longispina individuals from five eutrophic lakes in southern Sweden. We tested whether individuals collected before, during or following algal blooms differed in their reproduction and survival when experimentally exposed to microcystin‐producing cyanobacteria.

The concentration of microcystin in the lakes was significantly higher during summer and autumn compared to spring, but there were substantial differences between lakes. The reproductive output of individuals declined consistently over the season, and this decline was stronger for Daphnia collected during periods of, or from lakes with, high microcystin concentration. There was little evidence that individuals adapted to the toxin over the season.

The strong seasonal changes in body size, reproduction and survival in these D. longispina appear to be caused partly by variation in the abundance of toxin‐producing cyanobacteria. Populations were unable to adapt sufficiently quickly during summer and autumn to recover from the negative effects of microcystin. We therefore suggest that seasonal increases in tolerance to microcystin‐producing cyanobacteria have limited effects on the eco‐evolutionary dynamics between Daphnia and phytoplankton.
Adaptation and psychometric evaluation of the Stoma‐QOL questionnaire among Chinese rectal cancer patients with colostomy
Authors: Shao Lu , Lv Lin , Zheng Mei‐Chun , Huang Man‐Rong , Zhang Jun‐E 
Published: March 2022

AbstractAimThe purpose of this study was to translate the Stoma‐quality of life into Chinese and evaluate its psychometric properties in Chinese patients.BackgroundQuality of life is an important issue for patients with colostomy, and its appropriate and precise measurement is beneficial to promoting better care. The Stoma‐quality of life questionnaire has been widely used; however, the validity and reliability of its Chinese version has not been determined.DesignA cross‐sectional validation study was conducted.MethodsWe translated the Stoma‐quality of life into Chinese using standardized methods. Then it was psychometrically tested on a convenience sample of 513 patients with colostomy. Construct validity was evaluated via exploratory factor analysis and confirmatory factor analysis. Reliability was measured with Cronbach's alpha and the split‐half Spearman–Brown coefficient.ResultsThe content validity, the Cronbach's α coefficient and the Spearman–Brown split‐half reliability coefficient indicated adequate validity and reliability. The exploratory factor analysis yielded four common factors, and the cumulative variance contribution rate was 67.5%. Moreover, the confirmatory factor analysis showed a good model fit.ConclusionThis study confirmed that the Chinese version of Stoma‐quality of life is an effective and reliable measurement for evaluating the quality of life of patients with colostomy.
Le COVID‐19 en Amérique latine: les effets sur l'emploi et les revenus d'une crise sans précédent
Authors: BECCARIA Luis , BERTRANOU Fabio , MAURIZIO Roxana 
Published: March 2022

RésuméL'Amérique latine a connu en 2020 une crise de l'emploi sans précédent du fait de la pandémie de COVID‐19. Les auteurs analysent les effets de cet événement sur le marché du travail et les revenus, ainsi que les mesures prises par les pays de la région pour y faire face. Ils montrent que le fort ralentissement de l'activité économique a provoqué une chute brutale de l'emploi, des heures travaillées et des revenus. Certains groupes en ont pâti plus que d'autres. La reprise s'accompagne elle aussi d'un creusement des inégalités en matière d'emploi et de revenus.
COVID‐19 y situación laboral: Quién resiste, quién no y con qué consecuencias en términos de desigualdad
Authors: SOARES Sergei , BERG Janine 
Published: March 2022

ResumenLas respuestas estatales ante la pandemia de COVID‐19 han diferido en cuanto a alcance y diseño, con importantes implicaciones para el mercado laboral en general, y para grupos específicos de trabajadores. Utilizando datos de encuestas de población activa de siete países de renta media y alta, se analizan las transiciones laborales entre los dos primeros trimestres de 2020 en comparación con las de 2019. Se observa que la pandemia exacerbó las desigualdades en los siete países; cuando se favorecieron los subsidios salariales frente a otras formas de apoyo a los ingresos se redujo la volatilidad del mercado laboral.
¿Es realmente distinto esta vez? Impactos comparados de la crisis de la COVID‐19 y de la crisis financiera mundial de 2008–2009 en los mercados de trabajo
Authors: VERICK Sher , SCHMIDT‐KLAU Dorothea , LEE Sangheon 
Published: March 2022

ResumenEn 2020 la pandemia causó una crisis del mercado de trabajo más profunda que la generada por la crisis financiera mundial de 2009. Las medidas de contención fueron la principal causa del daño a los mercados de trabajo, que afectó particularmente a las economías de ingreso mediano, así como a ciertos sectores, como las actividades de alojamiento y servicio de comidas, y determinados grupos poblacionales, como las mujeres jóvenes. Frente a los procesos de ajuste del empleo impuestos en la crisis financiera mundial, durante la crisis de la COVID‐19 la inactividad ha aumentado más que la desocupación. Deben mantenerse las políticas de apoyo para evitar la desigualdad en la recuperación.
Prevalence of most common skin diseases in Europe: a population‐based study
Authors: Richard M.A. , Paul C. , Nijsten T. , Gisondi P. , Salavastru C. , Taieb C. , Trakatelli M. , Puig L. , Stratigos A. ,   EADV burden of skin diseases project team
Published: March 2022

AbstractBackgroundThe assessment of the prevalence of diseases is of primary importance in planning health policies. No complete data on the prevalence of skin diseases across European countries are available.ObjectiveTo estimate the prevalence of the most frequent skin conditions or diseases in 27 European countries (24 EU countries, plus Norway, Switzerland, and the United Kingdom).MethodsWe conducted a population‐based study on representative and extrapolable samples of the general population aged 18 years or more in each of the 27 countries surveyed. Participants were selected using stratified, proportional sampling with a replacement design. Data were collected using a web‐based online survey. All participants were asked to fill in a questionnaire with sociodemographic data and to declare if they have had one or more skin conditions or diseases during the previous 12 months.ResultsA total of 44 689 participants from 27 countries responded to the questionnaire, 21 887 (48.98%) men and 22 802 (51.02%) women. The proportion of participants who reported having suffered from at least one dermatological condition or disease during the previous 12 months was 43.35% (95% CI: 42.89%, 43.81%). The projection in the total population of the 27 countries included in the study resulted in 185 103 774 individuals affected by at least one dermatological condition or disease. Accordingly, we can estimate that more than 94 million Europeans complain of uncomfortable skin sensations like itch, burning, or dryness. The most frequent conditions were fungal skin infections (8.9%), acne (5.4%), and atopic dermatitis or eczema (5.5%). Alopecia, acne, eczema, and rosacea were more common in women, whereas men were more likely to suffer from psoriasis and sexually transmitted infections.ConclusionSkin diseases are an important public health concern. Their high prevalence has to be taken into account in planning access to dermatological care to address patient needs.