bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alveolar metabolite availability facilitates secondary infection byPseudomonas aeruginosain acutely injured lungs
Authors: Baker Jennifer M. , Flott Thomas L. , McLellan Laura A. , Bustos Ingrid G. , Guerrero Jose L. , Mendez Lina M. , Petouhoff Annastasia M. , Ranjan Piyush , Metcalf Joseph D. , McDonald Roderick A. , Falkowski Nicole R. , He Ying , Cala Mónica P. , Huffnagle Gary B. , Sjoding Michael W. , Reyes Luis F. , Stringer Kathleen A. , Dickson Robert P. 
Published: January 2025

AbstractSecondary bacterial pneumonia is a frequent complication of acute lung injury (ALI) and the acute respiratory distress syndrome (ARDS). Prior efforts to explain increased pneumonia risk in ALI/ARDS have focused on impaired immunity and bacterial virulence, but have overlooked the potential contribution of ecological factors within acutely injured lungs. Here, we show that lung injury profoundly alters the alveolar metabolic microenvironment, a change that can be exploited by a common pneumonia pathogen. In mice and humans, we found that growth ofPseudomonas aeruginosa(the most prominent respiratory pathogen in a retrospective cohort of patients with ARDS) is enhanced by increased alveolar concentrations of multiple ALI/ARDS-associated metabolites, which are derived from the blood and cross the compromised alveolar-capillary barrier during alveolar leak. Collectively, this work reveals an ecological mechanism by which pathogens may survive in the injured lung microenvironment and identifies new potential targets for secondary pneumonia prevention and treatment.
Short-Term Arcade-Action Video Gaming Enhances Working Memory Precision
Authors: Asgari Ali , Vahabie Abdol-Hossein , Rezayat Ehsan 
Published: January 2025

AbstractObjectiveAction video games are increasingly recognized for their potential to enhance cognitive capacities, including working memory (WM). However, most studies require extensive training or prior gaming experience. We examined whether a single, short-term session of “Snail Mail,” an arcade-action video game, could boost WM precision compared to a passive control condition.MethodThirty-six healthy adults (18–34 years) were randomly assigned to either play “Snail Mail” (Intervention) or watch episodes of “Shaun the Sheep” (Control) for 30 minutes. Two participants were excluded for unstable performance, yielding a final sample of 34. Participants completed a face emotion recognition memory task before and after the intervention, wherein the absolute error served as the primary metric of WM precision. A mixture model further characterized memory precision, and random guessing.ResultsThe Intervention group showed a significant reduction in absolute error from pre- to post-test, whereas the Control group exhibited a negative effect size, indicating worsened post-test performance. Mixture-model results revealed a significant increase in precision for the Intervention group.ConclusionThese findings indicate that a 30-minute arcade-action game session can meaningfully enhance WM precision, highlighting the feasibility of brief, targeted gaming interventions. Future research should explore longer-term outcomes, generalizability to other cognitive tasks, and optimal intervention protocols for sustained benefits.
Development of Proton Density Fat Fraction Micro-MRI for Non-Invasive Quantitative Assessment of Bone Marrow Changes with Age and Radiation in Mouse Models
Authors: Ghimire Hemendra , Malekzadeh Malakeh , Lim Ji Eun , Madabushi Srideshikan Sargur , Zampini Marco Andrea , Camacho Angela , Hu Weidong , Storme Guy , Malki Monzr M. Al , Hui Susanta K. 
Published: January 2025

AbstractBackground/ObjectivesBone marrow (BM) adipocytes are critical in progressing solid tumor metastases and hematological malignancies across pediatric to aging populations. Single-point biopsies remain the gold standard for monitoring BM diseases, including hematologic malignancies, but are limited in capturing the full complexity of loco-regional and global BM microenvironments. Non-invasive imaging techniques like Magnetic Resonance Imaging (MRI), could offer valuable alternatives for real-time evaluation of BM diseases in both preclinical translational and clinical studies.MethodsWe developed a preclinical proton density fat fraction (PDFF) MRI technique for quantitative BM composition assessment, focusing on fat fraction (FF) within mouse femurs. We validated this method using aging mice and young mice subjected to 10 Gy X-ray irradiation, compared with young unirradiated mice as controls. Water-fat phantoms (0% to 100% fat content) were used to optimize the imaging sequence, and immunohistochemical (IHC) staining with H&E validates equivalent adipose content in the femur BM regions.ResultsSignificant differences in FF were observed across age groups (p = 0.001 for histology and p = 0.0002 for PDFF) and between irradiated and control mice (p = 0.005 for histology and p = 0.002 for PDFF). A strong correlation (R2∼ 0.84) between FF values from PDFF and histology validates the accuracy of the technique.ConclusionsThese findings demonstrate the potential of PDFF MRI as a non-invasive real-time imaging biomarker for quantifying BM fat fraction in preclinical mice model studies, particularly in evaluating the effects of aging, disease progression, and irradiation therapy in pediatric and translational oncology research.
High-pressure Golgi neuronal staining for X-ray whole-brain imaging
Authors: Tang Qiaowei , Cai Xiaoqing , Liu Yu , Zhang Yu , Yan Xin , Zhou Feng , Zhang Jichao , Li Qian , Li Ke , Deng Biao , Wang Lihua , Li Jiang , Zhu Ying , Fan Chunhai , Hu Jun 
Published: January 2025

AbstractWhole-brain imaging has revolutionized neuroscience research, providing comprehensive insights into neural networks across the entire brain. This powerful approach has greatly advanced our understanding of brain functions and the mechanisms underlying various diseases. One primary challenge in whole-brain imaging technology is to achieve high-resolution observation of neural networks at large scales. Although Golgi method allows labeling random neurons in their entirety in the brain, visualizing individual dendritic trees, and tracing long-distance axonal projections, the lengthy processing time pose a limit on its use, i.e., staining a mouse whole-brain sample of just 300 mm³ takes over two weeks. Here, we developed a rapid staining technique for whole-brain neurons using high-pressure assisted Golgi (HP Golgi). This method significantly reduced the staining time for mouse whole-brain neurons from 16 days to only 4 days. We demonstrated the broad applicability of the HP Golgi method across various model organisms, achieving whole-brain neuronal staining in zebrafish, mice, and rats. Further, we successfully performed rapid staining of hippocampal neurons in an intact pig brain, which is difficult to achieve with the classic Golgi-Cox method. We also demonstrated that the combination of the HP Golgi method with synchrotron-based X-ray microscopy for high-resolution imaging of whole-brain neurons in mice. This HP Golgi method enables fast and high-resolution neuronal imaging in large model organisms, showcasing its broad applicability for diverse applications.
Thriving big data artificial intelligence: entrepreneurial leadership, proactive personality and affective commitment to innovate micro and small family businesses
Authors: Nusrat Ayesha , Zongming Zhang , Li Jie , Muhammad Muneeb Farhan 
Published: January 2025

PurposeThis study examines the impact of entrepreneurial leadership (EL) on Chinese micro and small family businesses’ (MSFBs) innovativeness. Drawing on the resource-based view, this research study further explores the intermediary roles of proactive personality (PP) and affective commitment (AC) between ELs’ and MSFBs’ innovativeness. Besides this, the present work proposes a novel contingency impact of big data-powered artificial intelligence (BDAI) between EL, PP and AC, which indirectly spurs MSFBs’ innovativeness.Design/methodology/approachThis study proposed a moderated mediation model using multi-wave, multi-source, time-lagged datasets of 380 employees from 190 Chinese MSFBs. We tested our hypotheses using structural equation modeling through the PLS technique.FindingsThe findings reveal a significant impact of EL on MSFB innovativeness, underscoring the pivotal intermediary roles of EL in driving MSFB innovativeness. Furthermore, BDAI emerges as a critical contingency factor, amplifying the effects of EL on both PP and AC to spur MSFBs’ innovativeness.Practical implicationsOur research offers several practical implications for Chinese MSFBs aiming to enhance innovativeness and competitive advantage. Firstly, understanding the direct impact of EL on MSFBs’ innovativeness provides valuable guidance for MSFB leaders. Secondly, recognizing the mediating roles of PP and AC underscores the importance of human and social capital in driving innovation within Chinese MSFBs. Thirdly, leveraging BDAI as a contingency factor can further augment the effects of EL on both PP and AC, thereby enhancing innovation outcomes. Thus, managers can capitalize on BDAI to gain actionable insights to increase MSFBs’ innovativeness.Originality/valueThis study enlightened how EL can develop MSFBs innovativeness through PP and AC. Our findings reveal that MSFBs can increase their innovation by leveraging PP and AC, leading to higher proactive provision in employees’ behavior. Subsequently, our results synchronized the exploration of BDAI as a novel insight for MSFB innovativeness. This shed light on a highly notable contribution to understanding BDAI to benefit MSFBs, acting as a critical contingency between EL, PP and AC.
Leader–member exchange and work–family conflict; the moderating role of emotional intelligence and supervisory support
Authors: Adu Isaac Nyarko , Darko Nana Yaa , Kyei-Frimpong Michael , Yeboah-Kordee Nana Serwah 
Published: January 2025

PurposeThis study sought to examine the interactional effect of emotional intelligence (EI) and supervisory support (SS) in the relationship between leader–member exchange (LMX) and work–family conflict (WFC).Design/methodology/approachThe study adopted the survey research design, where 286 employees in the Ghanaian financial services sector were sampled for this study. The data retrieved from the respondents were quantitatively analyzed using descriptive and inferential statistics with the aid of IBM SPSS Statistics (Version 26.0), SmartPLS (Version 4.0) and the Hayes PROCESS macro.FindingsAs hypothesized, this study revealed that LMX positively and significantly relates to WFC, while EI and SS significantly moderated the relationship between LMX and WFC.Practical implicationsThe findings of this study revealed the need for organizations to develop and implement policies that promote good leadership among employees.Originality/valueThis study fills the research gap in the Ghanaian financial service sector regarding the moderating effect of supervisory support and emotional intelligence in the nexus between leader–member exchange and employees’ work–family conflict.
Information seeking in a time of war: coping with stress in Lithuania during the Russia/Ukraine war
Authors: Wilson Thomas D. , Maceviciute Elena 
Published: January 2025

PurposeThe aim of the research reported here was to determine how Lithuanian citizens engaged in information-seeking behaviour in response to the stress caused by the Russian invasion of Ukraine.Design/methodology/approachAn interview survey was designed, using a semi-structured interview schedule. A convenience sample of 21 participants was obtained and the interviews lasted between 20 and 70 min. The schedule design was guided by the transactional theory of stress and coping and employed the Perceived Stress Scale.FindingsA majority of participants experienced moderate to high levels of stress associated with the war in Ukraine. Information seeking and discussing information found with family members and friends played a significant role in helping to moderate stress. Most of the participants understood more than one language and, consequently, were able to compare local information sources with international sources. Only five participants were active users of social media, the rest were critical of these sources. All participants valued those sources they believed to be reliable and truthful.Research limitations/implicationsThe small convenience sample of educated urban participants limits generalizability but provides indicative findings for future investigations into information behaviour during prolonged international conflicts.Practical implicationsThe study highlights the importance of media literacy in managing psychological stress during geopolitical tensions, demonstrating how strategic information seeking and social support can serve as effective coping mechanisms.Social implicationsThe research reveals psychological impacts of war beyond direct conflict zones, illustrating how communities develop collective emotional resilience through informed, critically engaged information practices.Originality/valueThe study provides unique insights by examining war-related stress in a neighbouring country not directly experiencing conflict, applying stress-coping theory to understand intricate information-seeking behaviours during a geopolitical crisis.
Senolytic treatment attenuates immune cell infiltration without improving IAV outcomes in aged mice
Authors: Luna Adrian , Chou Kai‐Neng , Wragg Kathleen M. , Worley Matthew J. , Paruchuri Nikhil , Zhou Xiaofeng , Blin Muriel G. , Moore Bethany B. , Salmon Morgan , Goldstein Daniel R. , Deng Jane C. 
Published: January 2025

AbstractAging is a major risk factor for poor outcomes following respiratory infections. In animal models, the most severe outcomes of respiratory infections in older hosts have been associated with an increased burden of senescent cells that accumulate over time with age and create a hyperinflammatory response. Although studies using coronavirus animal models have demonstrated that removal of senescent cells with senolytics, a class of drugs that selectively kills senescent cells, resulted in reduced lung damage and increased survival, little is known about the role that senescent cells play in the outcome of influenza A viral (IAV) infections in aged mice. Here, we tested if the aged mice survival or weight loss IAV infections could be improved using three different senolytic regimens. We found that neither dasatinib plus quercetin, fisetin, nor ABT‐263 improved outcomes. Furthermore, both dasatanib plus quercetin and fisetin treatments further suppressed immune infiltration than aging alone. Additionally, our data show that the short‐term senolytic agents do not reduce senescent markers in our aged mouse model. These findings suggest that acute senolytic treatments do not universally reverse aging related immune phenotype against all respiratory viral infections.
Characteristics of Soluble PD‐L1 and PD‐1 Expression and Their Correlations With Immune Status and Prognosis in Advanced Lung Cancer
Authors: Li Ran , Liang Hongge , Shang Ying , Yang Zhengwu , Wang Keqiang , Yang Donghong , Bao Jing , Xi Wen , Zhou Dexun , Ni Wentao , Gao Zhancheng , Mu Xinlin 
Published: January 2025

ABSTRACTPurposeOur study aims to evaluate the characteristics of serum soluble PD‐1 (sPD‐1) and soluble PD‐L1 (sPD‐L1) levels and their correlations with immune status and prognosis in advanced lung cancer patients.MethodsPatients diagnosed with advanced lung cancer based on histology or cytology in Peking University People's Hospital from July 2020 to November 2021 were enrolled. Clinicopathological data were recorded and analyzed. Treatment efficacy was evaluated according to RESIST 1.1 criteria. The serum levels of sPD‐L1 and sPD‐1 were detected by enzyme‐linked immunosorbent assay (ELISA). Lymphocyte subsets were measured by flow cytometry to evaluate the immune status of the patients.ResultsA total of 65 patients with advanced lung cancer were enrolled. sPD‐L1 level in lung cancer patients (15.67 ± 11.09 pg/mL, p = 0.001) was significantly higher than those in healthy controls (5.21 ± 4.46 pg/mL). sPD‐1 level did not show a significant difference between patients with lung cancer and healthy controls. sPD‐L1 level in patients with progressive disease (PD) was significantly higher than those with partial response (PR) (20.94 ± 8.91 vs. 13.14 ± 12.66 pg/mL, p = 0.033). In treatment‐naïve patients, sPD‐L1 level was negatively correlated with the lymphocyte ratio (correlation coefficient = −0.452, p = 0.014). Kaplan–Meier survival analysis showed that patients with low sPD‐L1 level had a significantly longer progression‐free survival (PFS) (10.4 vs. 5.7 months, p = 0.023). However, sPD‐1 level did not correlate with lymphocyte subsets or prognosis in overall patients with lung cancer. Subgroup analysis showed that prolonged PFS in patients with low sPD‐L1 level was exclusively shown in the NSCLC subgroup, not in the SCLC subgroup. In the subgroups of patients who subsequently received immunotherapy, low sPD‐L1 level was correlated with longer PFS in the overall patients and NSCLC patients, and low sPD‐1 level was correlated with longer PFS exclusively in NSCLC patients.ConclusionSerum sPD‐L1 level was higher in patients with advanced lung cancer than healthy individuals, which was negatively correlated with the proportion of lymphocytes and prognosis. Serum sPD‐1 level did not show significant difference between patients with lung cancer and healthy individuals, which showed no correlation with lymphocyte subsets and the prognosis of overall patients, except NSCLC patients receiving immunotherapy.
Beyond the screen: Dissecting the nexus of victimization and cyberhate among adolescents through excessive internet use, online interactions with strangers and parental restrictions
Authors: Korol Liliia , Blaya Catherine 
Published: January 2025

AbstractPrior research has established that being a target of offline and online victimization might function as a significant risk factor that increases the likelihood of adolescents' involvement in cyberhate. Yet, relatively little is known about the mediating and moderating mechanisms underlying this relationship. To fill this important gap in knowledge, the present study aims to examine (1) whether excessive Internet use and contact with unknown people online act as sequential mediators in the relationship between overall victimization and youth's involvement in cyberhate; and (2) whether restrictive parental mediation has any role to play in moderating this relationship. The findings suggest that adolescents who experience victimization are more likely to turn to using the Internet excessively, and consequently interact with strangers online, which in turn makes them more prone to becoming victim to cyberhate or spreading hateful content online themselves. Moreover, restrictive parental mediation was shown to exacerbate the link between excessive Internet use and adolescents' contacts with unknown people online, thereby putting them at higher risk of cyberhate involvement. The current study emphasizes the need for a balanced approach to parental mediation – one that fosters open communication, trust and the development of digital literacy skills.
Testing the Effectiveness of Synthetic Chemical Lures to Increase Fruit Bat‐Mediated Seed Dispersal in a Tropical Forest
Authors: Gelambi Mariana , Coto‐Pereira Yoselyn Pamela , Morales‐M Estefania , Whitehead Susan R. 
Published: January 2025

ABSTRACTTropical deforestation is occurring at alarming rates, creating an urgent need for global prioritization of restoration efforts. One potential forest recovery strategy is to boost seed‐dispersing animal activity (e.g., fruit‐eating bats) to increase seed availability in degraded areas. In this study, we investigated the efficacy of synthetic chemical lures in attracting fruit bats and enhancing seed rain in northeastern Costa Rica. The chemical lures were composed of a few volatile organic compounds commonly found in ripe bat‐dispersed fruits. Daily deployment of the chemical lures resulted in a significant increase in the capture of Carollia spp., a key neotropical seed disperser, but no detectable effect on other fruit bats. The 15‐day deployment of chemical lures also increased the average of total seeds collected in seed traps. However, the effect of lures explained only a small portion of the total variance in seed rain, highlighting the potential context‐dependency of these results. Still, in contrast to the previously studied essential oil‐based lures, synthetic lures hold the potential to be applied across a broader spectrum of restoration initiatives due to simplified manufacturing and improved reproducibility. Although additional research is essential to understand the full potential for use in restoration efforts, our study demonstrates the effectiveness of synthetic chemical lures in attracting fruit bats and potentially augmenting seed rain.
Integrated Mendelian Randomization and Single‐Cell Transcriptomics Analysis Identifies Critical Blood Biomarkers and Potential Mechanisms in Epilepsy
Authors: Shi Jianwei , Xie Jing , Yang Yanfeng , Fu Bin , Ye Zuliang , Tang Ting , Liu Quanlei , Xu Jinkun , Wei Penghu , Shan Yongzhi , Zhao Guoguang 
Published: January 2025

ABSTRACTBackgroundEpilepsy has a genetic predisposition, yet causal factors and the dynamics of the immune environment in epilepsy are not fully understood.MethodsWe analyzed peripheral blood samples from epilepsy patients, identifying key genes associated with epilepsy risk through Mendelian randomization, using eQTLGen and genome‐wide association studies. The peripheral immune environment's composition in epilepsy was explored using CIBERSORT. An epilepsy mouse model was established to validated the expression of key genes at the transcriptomic and proteomic levels through single‐cell analysis. Relevant pathways were verified. Finally, we developed a predictive model for antiepileptic drug response in epilepsy patients.ResultsWe found that CDC25B, DNMT1, GZMA, MTX1, and SSH2 expression decreases epilepsy risk, whereas FGD3, RAF1, and SH3BP5L increase it. Epilepsy patients exhibited an altered peripheral immune profile, notably with increased activated mast cells and decreased CD4 memory activated T cells and γδ T cells. Eight genes were significantly related to this immune environment. In the animal model, FGD3, SSH2, and DNMT1 were upregulated at both mRNA and protein levels in the hippocampus. FGD3 and SSH2 are specifically elevated in microglia and are primarily associated with actin regulation. The trained predictive model was deployed on an online platform.ConclusionsThis study elucidates key genes linked to epilepsy, delineates the epilepsy immune landscape, and highlights the interaction between these domains, providing insights into potential epilepsy mechanisms and treatments.