bluuebunny/crossref_metadata_2025_split huggingface dataset:
An M protein coiled coil unfurls and exposes its hydrophobic core to capture LL-37
Authors: Kolesiński Piotr , Wang Kuei-Chen , Hirose Yujiro , Nizet Victor , Ghosh Partho 
Published: January 2022

ABSTRACTSurface-associated, coiled-coil M proteins ofStreptococcus pyogenes(Strep A) disable human immunity through interaction with select proteins. However, coiled coils lack features typical of protein-protein interaction sites, and it is therefore challenging to understand how M proteins achieve specific binding, for example, with the human antimicrobial peptide LL-37, leading to its neutralization. The crystal structure of a complex of LL-37 with M87 protein, an antigenic M protein variant from a strain that is an emerging threat, revealed a novel interaction mode. The M87 coiled coil unfurled and asymmetrically exposed its hydrophobic core to capture LL-37. A single LL-37 molecule bound M87 in the crystal, but in solution recruited additional LL-37 molecules, consistent with a ‘protein trap’ neutralization mechanism. The interaction mode visualized crystallographically was verified to contribute significantly to LL-37 resistance in an M87 Strep A strain, and was identified to be conserved in a number of other M protein types that are prevalent in human populations. Our results provide specific detail for therapeutic inhibition of LL-37 neutralization by M proteins.
Genomic occupancy of the bromodomain protein Bdf3 is dynamic during differentiation of African trypanosomes from bloodstream to procyclic forms
Authors: Ashby Ethan , Paddock Lucinda , Betts Hannah L. , Miller Geneva , Porter Anya , Rollosson Lindsey M. , Saada Carrie , Tang Eric , Wade Serenity J. , Hardin Johanna , Schulz Danae 
Published: January 2022

AbstractTrypanosoma brucei, the causative agent of Human and Animal African trypanosomiasis, cycles between a mammalian host and a tsetse fly vector. The parasite undergoes huge changes in morphology and metabolism as it adapts to each host environment. These changes are reflected in the differing transcriptomes of parasites living in each host. While changes in the transcriptome have been well catalogued for parasites differentiating from the mammalian bloodstream to the insect stage, it remains unclear whether chromatin interacting proteins mediate transcriptomic changes during life cycle adaptation. We and others have shown that chromatin interacting bromodomain proteins localize to transcription start sites in bloodstream parasites, but whether the localization of bromodomain proteins changes as parasites differentiate from bloodstream to insect stage parasites remains unknown. To address this question, we performed Cleavage Under Target and Release Using Nuclease (CUT&RUN) timecourse experiments using a tagged version of Bromodomain Protein 3 (Bdf3) in parasites differentiating from bloodstream to insect stage forms. We found that Bdf3 occupancy at most loci increased at 3 hours following onset of differentiation and decreased thereafter. A number of sites with increased bromodomain protein occupancy lie proximal to genes known to have altered transcript levels during differentiation, such as procyclins, procyclin associated genes, and invariant surface glycoproteins. While most Bdf3 occupied sites are observed throughout differentiation, a very small number appear de novo as differentiation progresses. Notably, one such site lies proximal to the procyclin gene locus, which contains genes essential for remodeling surface proteins following transition to the insect stage. Overall, these studies indicate that occupancy of chromatin interacting proteins is dynamic during life cycle stage transitions, and provides the groundwork for future studies aimed at uncovering whether changes in bromodomain protein occupancy affect transcript levels of neighboring genes. Additionally, the optimization of CUT&RUN for use in Trypanosoma brucei may prove helpful for other researchers as an alternative to Chromatin Immunoprecipitation (ChIP).
Microglia-secreted TNF-α affects differentiation efficiency and viability of pluripotent stem cell-derived human dopaminergic precursors
Authors: Wenker Shirley D. , Gradaschi Victoria , Ferrari Carina , Farias Maria Isabel , Garcia Corina , Beauquis Juan , Zeng Xianmin , Pitossi Fernando J. 
Published: January 2022

ABSTRACTParkinson’s Disease is a neurodegenerative disorder characterized by the progressive loss of dopaminergic cells of thesubstantia nigra pars compacta. Even though successful transplantation of dopamine-producing cells into the striatum exhibits favourable effects in animal models and clinical trials; transplanted cell survival is low. Since every transplant elicits an inflammatory response which can affect cell survival and differentiation, we aimed to studyin vivoandin vitrothe impact of the pro-inflammatory environment on human dopaminergic precursors. We first observed that transplanted human dopaminergic precursors into the striatum of immunosuppressed rats elicited an early and sustained activation of astroglial and microglial cells after 15 days post-transplant. This long-lasting response was associated with Tumor necrosis factor alpha expression in microglial cells.In vitroconditioned media from activated BV2 microglial cells increased cell death, decreased Tyrosine hydroxylase -positive cells and induced morphological alterations on human neural stem cells-derived dopaminergic precursors at two differentiation stages: 19 days and 28 days. Those effects were ameliorated by inhibition of Tumor necrosis factor alpha, a cytokine which was previously detectedin vivoand in conditioned media from activated BV-2 cells. Our results suggest that a pro-inflammatory environment is sustained after transplantation under immunosuppression, providing a window of opportunity to modify this response to increase transplant survival and differentiation. In addition, our data show that the microglia-derived pro-inflammatory microenvironment has a negative impact on survival and differentiation of dopaminergic precursors. Finally, Tumor necrosis factor alpha plays a key role in these effects, suggesting that this cytokine could be an interesting target to increase the efficacy of human dopaminergic precursors transplantation in Parkinson’s Disease.
Exercise reprograms the inflammatory landscape of multiple stem cell compartments during mammalian aging
Authors: Liu Ling , Kim Soochi , Buckley Matthew T. , Reyes Jaime M. , Kang Jengmin , Tian Lei , Wang Mingqiang , Lieu Alexander , Mao Michelle , Rodriguez-Mateo Cristina , Ishak Heather , Jeong Mira , Wu Joseph C. , Goodell Margaret A. , Brunet Anne , Rando Thomas A. 
Published: January 2022

ABSTRACTExercise has the ability to rejuvenate stem cells and improve tissue homeostasis and regeneration in aging animals. However, the cellular and molecular changes elicited by exercise have not been systematically studied across a broad range of cell types in stem cell compartments. To gain better insight into the mechanisms by which exercise affects niche and stem cell function, we subjected young and old mice to aerobic exercise and generated a single cell transcriptomic atlas of muscle, neural and hematopoietic stem cells with their niche cells and progeny.Complementarily, we also performed whole transcriptome analysis of single myofibers from these animals. We identified common and unique pathways that are compromised across these tissues and cell types in aged animals. We found that exercise has a rejuvenating effect on subsets of stem cells, and a profound impact in the composition and transcriptomic landscape of both circulating and tissue resident immune cells. Exercise ameliorated the upregulation of a number of inflammatory pathways as well as restored aspects of cell-cell communication within these stem cell compartments. Our study provides a comprehensive view of the coordinated responses of multiple aged stem cells and niche cells to exercise at the transcriptomic level.
Enhancing competitive advantage and financial performance of consumer-goods SMEs in export markets: how do social capital and marketing innovation matter?
Authors: Jeong So Won , Chung Jae-Eun 
Published: January 2022

PurposeBuilding on the resource-based view (RBV), this study examines the differential roles of internal and external social capital (SC) in enhancing the marketing innovation (MI), competitive advantage and financial performance of Korean small and medium-sized enterprises (SME) in export markets.Design/methodology/approachIn total, 197 valid cases were obtained from Korean manufacturing SMEs in the consumer goods sector. Then, a path analysis was employed to test the proposed hypotheses.FindingsFirst, SME internal SC positively influenced MI, whereas external SC positively influenced foreign distributor's MI (or foreign distributor MI). Second, SME MI was positively associated with foreign distributor MI. Third, no statistical differences were found in the strength of the paths from foreign distributor and SME MI to competitive advantage. Meanwhile, foreign distributor MI positively affected financial performance more than SME MI. Fourth, SME competitive advantage predicted a positive financial performance. Finally, the effect of SME MI on financial performance had an inverted “U” shape.Originality/valueThis study fills a research gap in the MI literature by identifying MI in terms of its actors (SMEs and their foreign distributors) and examining the differential roles of internal and external SC in MI.
Causal effects of corporate taxes on private firms' earnings management: a regression discontinuity analysis
Authors: Kong Gaowen 
Published: January 2022

PurposeThe authors emphasize the information role of earnings management and how it may be used to “mislead some stakeholders about the underlying economic performance of the company or to influence contractual outcomes that depend on reported accounting numbers.” Specifically, the authors examine the causal effect of tax incentives on private firms' earnings management based on a corporate tax reform in China.Design/methodology/approachIn December 2001, China implemented a tax collection reform which moved the collection of corporate income taxes from the local tax bureau to the state tax bureau. This reform results in exogenous variations in the effective tax rate among similar firms established before and after 2002. The authors apply a regression discontinuity design and use the generated variation in the effective tax rate to investigate the impact of taxes on firm earnings management.FindingsThe authors find that tax reduction substantially increases private firms' incentives to manage earnings information, and such effect is particularly pronounced when tax collection intensity and government interventions are low. Further evidence shows that lower tax rates stimulate firms' investment, inventory turnover and recruitment of skilled human capital. A plausible mechanism is that private firms signal a promising outlook by managing earnings to attain greater financing and improve investment/operation levels when financial constraints are removed.Originality/valueFirst, the authors present the causal effects of tax incentives on private firm's earnings management, which deepens the authors’ understanding on the determinants of firm's earnings information production. Second, this study also contributes to the literature on tax-induced earnings management. Third, the authors believe that this topic offers clear policy implications and would be of particular interest to regulators.
A benchmark analysis of the quality of distributed additive manufacturing centers
Authors: Verna Elisa , Maisano Domenico Augusto 
Published: January 2022

PurposeNowadays, companies are increasingly adopting additive manufacturing (AM) technologies due to their flexibility and product customization, combined with non-dramatic increases in per unit cost. Moreover, many companies deploy a plurality of distributed AM centers to enhance flexibility and customer proximity. Although AM centers are characterized by similar equipment and working methods, their production mix and volumes may be variable. The purpose of this paper is to propose a novel methodology to (1) monitor the quality of the production of individual AM centers and (2) perform a benchmarking of different AM centers.Design/methodology/approachThis paper analyzes the quality of the production output of AM centers in terms of compliance with specifications. Quality is assessed through a multivariate statistical analysis of measurement data concerning several geometric quality characteristics. A novel operational methodology is suggested to estimate the fraction nonconforming of each AM center at three different levels: (1) overall production, (2) individual product typologies in the production mix and (3) individual quality characteristics.FindingsThe proposed methodology allows performing a benchmark analysis on the quality performance of distributed AM centers during regular production, without requiring any ad hoc experimental test.Originality/valueThis research assesses the capability of distributed AM centers to meet crucial quality requirements. The results can guide production managers toward improving the quality of the production of AM centers, in order to meet customer expectations and enhance business performance.
Mobile app users' privacy concerns: different heuristics for privacy assurance statements in the EU and China
Authors: Hudson Sarah , Liu Yi 
Published: January 2022

PurposeAs mobile apps request permissions from users, protecting mobile users' personal information from being unnecessarily collected and misused becomes critical. Privacy regulations, such as General Data Protection Regulation in the European Union (EU), aim to protect users' online information privacy. However, one’s understanding of whether these regulations effectively make mobile users less concerned about their privacy is still limited. This work aims to study mobile users' privacy concerns towards mobile apps by examining the effects of general and specific privacy assurance statements in China and the EU.Design/methodology/approachDrawing on ecological rationality and heuristics theory, an online experiment and a follow-up validation experiment were conducted in the EU and China to examine the effects of privacy assurance statements on mobile users' privacy concerns.FindingsWhen privacy regulation is presented, the privacy concerns of Chinese mobile users are significantly lowered compared with EU mobile users. This indicates that individuals in the two regions react differently to privacy assurances. However, when a general regulation statement is used, no effect is observed. EU and Chinese respondents remain unaffected by general assurance statements.Originality/valueThis study incorporates notions from fast and frugal heuristics end ecological rationality – where seemingly irrational decisions may make sense in different societal contexts.
Smart cities and quality of life: a quantitative analysis of citizens' support for smart city development
Authors: Chen Zhaoyu , Chan Irene Cheng Chu 
Published: January 2022

PurposeThis study examined a tourism destination, Macao, a fast-progressing smart city under development, vis-à-vis a set of smart city quality of life (SCQOL) domains and verified their effects on citizens' attitudes, perceptions and support for citizen-centric smart city development (SCD).Design/methodology/approachThis study adopted a quantitative approach. In particular, a questionnaire survey was used to collect data from Macao citizens. Factor analysis was used to identify SCQOL domains, while multiple linear regression and cluster analysis were used to achieve the research objectives.FindingsThis study identified five SCQOL domains: smart environment, smart people, smart livelihood, smart economy and economic policy, and smart mobility. Each of the domains had a different influence on citizens' attitudes and support for SCD. Three citizen segments (passive, neutral and enthusiastic supporters) were identified.Practical implicationsThe five SCQOL domains, their effects on citizens' support for SCD and the three citizen segments identified can help implement the appropriate measures to enhance the target groups' SCD. The findings are also of practical value in evaluating the citizen-centric approaches on smart progress in other contexts.Originality/valueThe concept of smart technology has been widely applied to all aspects of city development. The main goal of SCD is to enhance citizens' quality of life. However, most studies have only explored smart cities and quality of life in isolation. Grounded on citizen centrality, this study contributes to the literature on SCD by proposing a new concept of SCQOL, identifying the domains constituting SCQOL and their influence on citizens' support for SCD.
Effects of political networking capability and strategic capability on exploratory and exploitative innovation: evidence from traditional manufacturing firms in China
Authors: Wang Guanhua , Wang Yaqin , Ju Xiaowei , Rui Xueqin 
Published: January 2022

PurposeThis study examines the effect of political networking capability (PNC) and strategic capability on exploratory innovation/exploitative innovation through the mediation of absorptive capability (AC).Design/methodology/approachUsing empirical survey data collected from 153 traditional manufacturing firms (TMFs) in China, the authors apply partial least squares structural equation modeling (PLS-SEM) combined with mediation analyses to test hypotheses.FindingsPNC has a higher impact on exploratory innovation than exploitative innovation through AC. The authors thus provide novel empirical insights into independent variables of firms' ambidextrous innovation and their implementation mechanisms.Research limitations/implicationsThe authors highlight a unique situation of China and contribute to the literature on PNC and AC. The findings demonstrate that AC plays an important role in configuring government-obtained external resources into new products, thus influencing ambidextrous innovation strategic decisions.Practical implicationsTMFs' executives should enhance PNC to obtain more resources to conduct exploratory and exploitative innovation. Government officials and policymakers should strengthen the supervision of TMFs' innovation activities and adopt effective measures to ensure that TMFs could conduct more exploratory innovation as governments expected.Originality/valueThis study provides new insights by bridging research gaps in the literature and advances the insights of how TMFs' PNC/strategic capability directly and indirectly fosters exploratory and exploitative innovation via the mediating role of AC in China.
Understanding the different types of UGC participants and social context for fashion brands: insights from social media platforms
Authors: Naeem Muhammad , Ozuem Wilson 
Published: January 2022

Purpose
The study aims to look at the types of social media participants through user-generated content (UGC) and how this leads to brand engagement in a fashion retail context. In doing so, it explores the effects of social context of brand-related content on other social media users, which promotes socially influenced consumer brand engagement (SICBE) in social media settings.

Design/methodology/approach
The study postulates the existence of social realities as consistent with social constructivism, with multiple realities of social influence outlined on the basis of ontological relativism. To fulfil the proposed research objectives, research data were gathered from professional and social participants based on specific inclusion criteria, purposive sampling technique and a semi-structured interview method.

Findings
Findings highlighted various types of UGC participants with differing objectives in their use of UGC; these participant types are passive, creators, critics and collectors/consumers. The study uncovered many social context that can increase the effectiveness of UGC. The social context is explored through social trust, Fashion UGC expertise and relevance. These UGC participants and social context can foster SICBE in a fashion retail context.

Originality/value
This study proposes a holistic framework which highlighted the role of UGC participants and social context can foster SICBE in a fashion retail context.
Structure and properties of modified gypsum binder
Authors: Yakovlev G.I. , Gordina A. , Drochytka Rostislav , Buryanov A.F. , Smirnova Olga 
Published: January 2022

PurposeThe purpose of the study is regarding the development of eco-oriented technologies for obtaining the building gypsum materials with the involvement of industrial by-products or waste.Design/methodology/approachThe scanning electron microscopy, X-ray microanalysis and IR spectral analysis were used to study the structure of gypsum matrix. The method of comparison of modified and unmodified gypsum matrix was used. Physical modeling of gypsum matrix crystallization is used to study changes in the morphology of hydration products.FindingsThe experimental results show that the addition of technical soot into a gypsum binder leads to a change in the morphology of crystalline hydrates of calcium sulfate dihydrate. Results of the scanning electron microscopy, X-ray microanalysis and IR spectral analysis confirm the change of physical and mechanical characteristics of the gypsum binder due to the structural modification of the gypsum matrix with ultrafine carbon soot. The achieved degree of the structural modification of the gypsum matrix is compatible with the results obtained when the gypsum binder was modified with dispersions of carbon nanotubes.Originality/valueThe morphology of the crystalline hydrates of the gypsum matrix with the addition of 0.04%, 0.06% and 1% of the carbon soot is characterized by the transition of the classical needle-like structure of gypsum dihydrate to the lamellar structure of increased density. One can observe the formation of intergrowths around ultrafine carbon soot particles. The studied carbon additive can improve strength characteristics of the gypsum matrix.
Corporate governance and extent of corporate sustainability practice: the role of investor activism
Authors: Gold Nusirat Ojuolape , Taib Fauziah Md. 
Published: January 2022

Purpose
Following the unceasing pressure on companies to adopt sustainable business practices to mitigate climate effect, this study aims to examine corporate governance (CG) attributes and role of activist investors in influencing extensive sustainability practice for firms in the developed and emerging climes.

Design/methodology/approach
Using a panel ordered probit regression analysis for 368 companies over 2016 to 2019, the study examined CG attributes that drive extensive corporate sustainability practice. The study addressed endogeneity bias using STATA Extended panel ordered probit regression model with endogenous covariates.

Findings
The result showed CG attributes is critical for firms, and activist investors play a critical role in driving extensive sustainability practice. Findings further reveal the extent of adoption is relatively low in the emerging climes but showed sign of improvement over the years examined.

Research limitations/implications
The study focused mainly on larger firms operating in different sectors globally. Hence, findings cannot be generalized for small sized entities.

Practical implications
The study provides an insightful explanation regarding the extensive sustainability practices and the vital role assumed by activist investors.

Social implications
The increasing number of companies responding to Carbon Disclosure Project and consequent improvement in scores indicates a corporate commitment to ensuring a sustainable future.

Originality/value
This research offers significant insights to the extent discussion on attributes of CG critical for sustainability practice. The findings ascertain useful tools to aid the continued adoption of sound sustainability practices around the globe.