bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED: Wearing compression socks results in significantly lower pulse wave velocity in human peripheral arteries
Authors: Morinaga Kosuke , Nakahara Masako , Matsura Kotaro , Ishihara Shigekazu , Idobata Yasuhiro , Kobata Takafumi , Tsuji Toshio 
Published: June 2022

Abstract

The authors have requested that this preprint be removed from Research Square.
Prevalence and mechanism of carbapenem-resistant genes in Acinetobacter baumannii
Authors: Khalid Komal , Kashif Amer Sohail , Amir Afreenish , Waris Muhammad Asim , Gul Sania , Gilani Syed Omer , Zafar Saima , Nawaz Eid , Ullah Nadeem , Ikram Aamer 
Published: June 2022

Abstract
BackgroundGram-negative Acinetobacter baumannii is an opportunistic pathogen, being highly reported for causing various nosocomial infections worldwide. The present study aimed to characterize the presence of carbapenem-resistant genes among multidrug-resistant Acinetobacter baumannii strains, isolated from the tertiary care hospital in Pakistan. MethodsBiochemical tests were performed for the initial identification of bacterial strains and further confirmed by MALDI-TOF mass spectrometry. Antibiotic susceptibility testing was done by using the standard Kirby-Bauer disk diffusion method, and carbapenem resistance determinants were further screened Metallo-beta-lactamase manufacture through Imipenem/Imipenem-EDTA-CDT. All 46 imipenem-resistant and 44 MBL positive isolates of A. baumannii were further amplified by PCR. ResultsThe OXA-51-like gene was detected in 45 strains (97.82%), while OXA-23-like was detected in 44 (95.65%) strains of carbapenem-resistant Acinetobacter baumannii. Moreover, New Dehli MBL, blaNDM gene was identified in four (9.09%) MBL positive carbapenem-resistant Acinetobacter baumannii (CRAB) isolates. All blaNDM isolates were co-expressed by the OXA-23-like gene while OXA-51-like was absent in one blaNDM positive strain of carbapenem-resistant Acinetobacter baumannii isolates. ConclusionA high prevalence of OXA-51-like and OXA-23-like genes was seen among the carbapenem-resistant Acinetobacter baumannii isolates, followed by blaNDM-1. The study findings showed the significant presence of carbapenem-resistant genes in MDR Acinetobacter baumannii strains, which may lead to clinical therapeutic failures.
Comparative transcriptomics reveals key genes contributing to the differences in drought tolerance among three cultivars of Foxtail millet (Setaria italica)
Authors: Guo Yue , Hao Dongdong , Wang Xiaoxia , Wang Hui , Wu Zehao , Yang Pu , Zhang Ben 
Published: June 2022

Abstract
Foxtail millet ( Setaria italica L.) is an important food crop with strong drought stress tolerance. The response of foxtail millet to drought stress is a complex regulatory network, and the physiological mechanism of foxtail millet's adaptation to drought stress is still unclear. This study investigated three different cultivars of foxtail millet with different drought resistance. Drought stress reduced the water content and chlorophyll content of foxtail millet leaves and increased the content of MDA and soluble protein. From strong to weak drought resistance order was Jigu39, Jingu21, and Longgu16. The transcriptome analysis of these three cultivars was carried out. 2954, 1531 and 2344 differentially expressed genes under drought stress were identified in Jigu39, Jingu21 and Longgu16. The GO and KEGG pathway analysis identified DEGs significantly enriched in photosynthesis, chlorophyll metabolism, amino acid metabolism, and carbohydrate metabolism in all three cultivars. In addition, we identified 46 genes whose trends of transcription change were consistent with the drought resistance trends among three cultivars of foxtail millet. Among them, 32 genes were first identified related to drought response in foxtail millet, which was worth for further research.
Identification of stages of Dysplasia using Graf type Ultrasound Images
Authors: Pavithra S , R Vanithamani , Justin Judith 
Published: June 2022

Abstract
Dysplasia is a congenital condition of the muscles and joints that occurs in the fetus. In hip dysplasia, the femoral head of the upper femur does not fully cover the hip portion of the bone, and as a result, the hip can become partially or fully dislocated. In addition to joint immobility and osteoarthritis, it can also cause prolonged pain in the joints. Infants with dysplasia should be treated as soon as possible to avoid troublesome experiences. Ultrasound examination of the hip is used to detect dysplasia, as it is non-invasive and less harmful to the body. However, ultrasound images are limited by speckle noise. Hence, speckle reduction is crucial to improve the image quality and for extracting features. Ultrasound images with speckle noise can be de-speckled using a hybrid model composed of Speckle Reduction Anisotropic Diffusion (SRAD) and Deep Neural Network (DNN). With respect to Signal to Noise Ratio (SNR), Peak Signal to Noise Ratio (PSNR), Mean Square Error (MSE) and Structural Similarity Index (SSIM), the performance is compared with that of state-of-the-art methods for speckle reduction. It has been observed that the proposed model is efficient in reducing speckle noise. The work emphasizes the classification of hip dysplasia. Denoising the image enables the extraction of statistical features, including skewness, kurtosis, alpha angle, mean, standard deviation, and convexity. For the purpose of classifying stages of dysplasia, the extracted features are fed into classifiers like Boosted Trees, Random Forest, Multi-class SVM, and Naive Bayes. The performance of the classifiers is measured by the sensitivity, specificity, accuracy, precision, Dice Coefficient (DC), Jaccard index (JI), and F1 score. The Boosted Trees algorithm performs better than the other classifiers used in this study.
Lactobacillus rhamnosus GG ameliorates noise-induced cognitive deficits and systemic inflammation in rats by modulating the gut-brain axis
Authors: Li Xiaofang , Zheng Pengfang , Cao Wa , She Xiaojun , Yang Honglian , Ma Kefeng , Wu Fangshan , Gao Xiujie , Fu Yu , Yin Jiayi , Wei Fei , Jiang Shoufang , Cui Bo 
Published: June 2022

Abstract
Background
Environmental noise exposure is linked to neuroinflammation and imbalance of the gut microbiota. Promoting gut microbiota homeostasis may be a key factor in relieving the deleterious non-auditory effects of noise. This study aimed to investigate the effect of Lactobacillus rhamnosus GG (LGG) intervention on noise-induced cognitive deficits and systemic inflammation in rats.
Methods
Learning and memory were measured using the Morris water maze, while 16S rRNA sequencing and gas chromatography-mass spectrometry were used to analyze the gut microbiota and short-chain fatty acid (SCFA) content. Endothelial tight junction proteins and serum inflammatory mediators were assessed to explore the underlying pathological mechanisms.
Results
The results indicated that LGG intervention ameliorated noise-induced memory deterioration, promoted the proliferation of beneficial bacteria, inhibited harmful bacteria, improved dysregulation of SCFA-producing bacteria, and regulated SCFAs levels. Mechanistically, noise exposure led to a decrease in tight junction proteins in the gut and hippocampus and an increase in serum inflammatory mediators, which were significantly alleviated by LGG intervention.
Conclusions
Taken together, LGG intervention reduced gut bacterial translocation, restored gut and blood-brain barrier functions, and improved gut bacterial balance in rats exposed to chronic noise, thereby protecting against cognitive deficits and systemic inflammation by modulating the gut-brain axis.
Increased complexity in volcanic architecture along waning hotspots
Authors: Tapu Al-Tamini , Ubide Teresa , Vasconcelos Paulo 
Published: June 2022

Abstract
Mantle plumes are key drivers of intraplate volcanism on Earth. Hotspot flux controls the lifetime of volcanism, yet its impact on magma plumbing architecture, composition of erupted products, and eruptive styles remain poorly constrained. Here, we combine geochemistry and geochronology on Earth's longest continental hotspot chain to assess the effect of waning plume activity on pre-eruptive magma transfer, storage, and differentiation. We identify two groups of age-progressive Cenozoic volcanoes across eastern Australia. The Northern volcanoes are older and "long-lived" (3.5–7 Ma activity) and erupted high volumes (>800 km3) of magmas with bimodal compositions, including relatively homogeneous basalts with aphyric texture (~3 vol% phenocrysts). In contrast, the southern volcanic chain splits into two parallel tracks, where volcanoes become "short-lived" (~1.5 Ma or less), erupting <300 km3 of more evolved basalts that show greater compositional and textural complexity (~12 vol% zoned phenocrysts). The inflection point coincides with a change in plate motion, suggesting geodynamic controls on plume flux, which in turn controls plumbing system architecture. Our results suggest waning flux leads to increasingly complex feeder systems and enhanced magma differentiation. Similar trends in hotspot tracks globally suggest that hotspot flux plays a crucial role in the evolution of intraplate volcanoes.
Validated LC-MS/MS method for the simultaneous analysis of veterinary drugs in cultured meat media
Authors: Gu Song-Yi , Kim Jong Jib , Lee Jongsoo , Park Kyung-Ho , Lee Sang-Yoon , Kim Sang-Gu 
Published: June 2022

Abstract
The development of alternative meat, such as cultured meat, is of current interest due to its potential to solve various problems common to the livestock industry. Cultured meat is defined as in vitro, lab-grown, or synthetic meat, and is produced through the differentiation and culture of animal cells. To increase the efficiency of this culture veterinary drugs are commonly employed. It is therefore necessary to develop a method that allows the amounts of veterinary drugs, growth-promoting hormones, and antibiotics to be determined in cultured meat, to ultimately ensure an adequate safety profile. In this study, we report the development of an analytical method based on liquid chromatography–tandem mass spectrometry for the simultaneous analysis of various veterinary drugs. Validation of the developed method indicated a high accuracy and precision, in addition to a linearity of 0.9868–0.9999. Using this method, the veterinary drugs present in samples of culture media were analyzed. Importantly, penicillin was detected in the range of 2 to 44,259 mg/L, and no other veterinary drugs were detected. It is therefore expected the implementation of this method will improve the safety profile of the cultured meat market, in addition to securing the safety of new food technologies.
TOP2A Serves as a Prognostic Marker Associated with Immune Infiltration in Hepatocellular Carcinoma
Authors: Su Qiuming , Zhang Shengning , Ran Jianghua 
Published: June 2022

Abstract
Background
TOP2A is a key enzyme that controls the topological state of DNA during DNA replication and transcription. We aim to explore the role of TOP2A in hepatocellular carcinoma (HCC).
Methods
The expression and prognostic value of TOP2A in HCC were analyzed by TIMER, TCGA, GEO, HPA, GEPIA and Kaplan-Meier plotter databases. The potential molecular mechanism of TOP2A in HCC was researched by GSEA software. The construction of the TOP2A gene co-expression network was completed by the cBioPortal database. The relationship between TOP2A and immune cell infiltration in HCC was explored through the CIBERSORT and TIMER databases.
Results
TOP2A was overexpressed in HCC and was associated with a poor prognosis. High expression of TOP2A was associated with worse pathological grade, deeper invasion depth, and advanced TNM stage, which was an independent unfavorable prognostic factor for HCC (HR = 1.863, P = 0.004). The GSEA results indicated that TOP2A was closely related to tumorigenesis and cellular metabolism. Furthermore, TOP2A and its co-expressed genes were positively associated with tumor-infiltrating immune cells. These co-expressed genes were independent prognostic factors, and the expression of these genes combined with macrophage levels helped to predict prognosis of HCC. In terms of immune infiltration, HCC patients with high TOP2A expression that the proportions of resting memory CD4 + T cells, activated CD4 + T cells, follicular helper T cells, regulatory T cells, macrophages, and neutrophils were significantly increased. The copy number variations of TOP2A affected the level of tumor-infiltrating immune cells. The expression of TOP2A was positively correlated with immune gene markers of T cell exhaustion in the HCC microenvironment.
Conclusion
TOP2A is a prognostic molecular marker of HCC, which is related to immune cell infiltration.
Examining master of nursing specialist postgraduates’ perception of educational service quality in clinical practice base: The SERVPERF model
Authors: Qin Lumei , Shi Chongqing , Li Bingjie , Ma Li 
Published: June 2022

Abstract
Background: Previous studies have investigated the perception of the service quality on education and health. However, there has been little research on Master of Nursing specialist (MNS) postgraduates’ perception of the educational service quality in the clinical base.Methods: A cross-sectional study was conducted by using convenience sampling, and 176 master of nursing specialist postgraduates from September-November 2020 in clinical practice base in six Provinces were selected for the study and surveyed using the general information questionnaire and the modified SERVPERF scale. Multiple linear regression was used to explore associated factors of educational service quality in clinical practice base on the master of nursing specialist postgraduates.Results: The Cronbach’s α coefficient and split-half reliability of the modified SERVPERF scale were satisfied. Master of nursing specialist postgraduates’ perceptions of each dimension of the educational service quality in the clinical practice base was 76.00%, 75.97%, 76.88%, 79.67%, and 77.75%, respectively, which was moderate. The items where masters of nursing specialist postgraduates rated their perception highest contain item D6, item D3, item B4, item A3, and item A4, which show they were satisfied with the quality of educational services; the 5 items with the lowest scores were item B3, item B2, item B6, item B7, and item E3, which stand for the weakest links in the clinical practice base. Multiple linear regression showed that practical teaching time was undertaken by clinical practice base and the level of clinical practice base were influential factors in the degree of master of nursing specialist postgraduates’ perception of educational service quality on clinical practice base (p < 0.05).Conclusion: This study showed that master of nursing specialist postgraduates had a moderate level of perception of educational service quality in clinical practice base, and practical teaching time was undertaken by clinical practice base and the level of clinical practice base were influential factors. Clinical practice base should pay attention to the problem of master of nursing specialist postgraduates’ perception of educational service quality and prevent and intervene early to improve the quality of educational service.
Development of a prosthetic device for swimming based on engineering design approach
Authors: Francisco Marta Gomes , Junior Osiris Canciglieri , Okumura Maria Lucia Miyake , Sant'Anna Angelo Marcio Oliveira 
Published: June 2022

Abstract
Engineering design with innovative characteristics that offer additional benefits to the customer is a process consisting of complex activities that require research, planning, and systematic methods. A prominent product development process has been driven by technological innovations used to assist the disabled in acquiring greater autonomy and performing daily tasks. Assistive product development has ensured the disabled more excellent prospects, contributing to providing or expanding the functional abilities of people with disabilities, resulting in the promotion of independent living and inclusion. This paper aims to develop a prosthetic device to aid patients with hand agenesis in practicing swimming based on the engineering design approach. The assistive product development was made based on the four phases: (i) demand perception, (ii) product projection, (iii) product characterization, and (iv) optimization. In addition, it was used for Monte Carlo simulation, multivariate analysis, and tolerance interval analysis techniques in the product development phases. We developed this approach as an alternative to classical engineering design used in the additive manufacturing process for prosthetic devices. The assistive product set aims to significantly stimulate the hand agenesis patient in physical activity, representing one of the doors of entry for social inclusion, providing a better quality of physical and emotional life for the individual with a disability.
Response to brolucizumab treatment for refractory serous pigment epithelial detachment secondary to polypoidal choroidal vasculopathy
Authors: Nam Seung Wan , Byun Zeeyoon , Ham Don-Il , Kong Mingui 
Published: June 2022

Abstract
Retinal pigment epithelial detachments (PEDs) secondary to polypoidal choroidal vasculopathy (PCV) are refractory to previous anti-VEGF treatment. This study evaluated the effect of brolucizumab on refractory serous PEDs secondary to PCV. This retrospective study included 26 eyes of 26 patients. Intravitreal brolucizumab 6.0 mg was administered, followed by pro re nata (PRN) retreatment at monthly follow-ups. All patients underwent spectralis domain optical coherence tomography (SD-OCT), fluorescein angiography, and indocyanine green angiography before the first brolucizumab injection. SD-OCT was repeated at follow-up visits. The height and width of the serous PEDs, measured using SD-OCT, were analyzed. The number of previous anti-VEGF injections was 12.3 ± 15.0. During brolucizumab treatment, anatomical improvement was achieved and maintained in the height and width of the PEDs (p < 0.05). However, the visual outcome did not improve significantly (p > 0.05). A good response was achieved in 69.2% of eyes at 1 month and at the last visit. Relapse and complete resolution were observed in 27.8% and 23.1% of patients, respectively. Intraocular inflammation, vascular obstruction, and retinal pigment epithelial tears were not observed. The results indicate that intravitreal brolucizumab may be an effective and safe treatment option for refractory serous PEDs in patients with PCV.
Influence of residual stress on critical rotational speed of circular saw blades with different structures
Authors: Li Bo , Yu Mingyang , Zhang Boyang , Zhang Qingdong 
Published: June 2022

Abstract
Circular saw blade is an important tool for wood processing. The controllable residual stress formed by tensioning process has an obvious influence on the critical rotational speed of circular saw blade. With the diversification of circular saw blade structure and tensioning method, the influence of residual stress field on critical rotational speed of circular saw blade deserves further study. In this paper, four types of circular saw blades were built by finite element method. Circular elastic thermal expansion zone and annular elastic thermal expansion zone were used to produce a certain distribution of residual stress field for circular saw blade. Critical rotational speed of circular saw blade with residual stress field was obtained by finite element method and vibration theory. The theoretical analysis results were shown as follows. For the four types of circular saw blades in this paper, when the tangential tensile stress with sufficient value is formed on the outer edge of circular saw blade, the critical rotational speed of circular saw blade will be increased compared with circular saw blade with no residual stress and the critical rotational speed will be increased with the tangential tensile stress.
The relationship between red blood cell distribution width and islet β-cell function indexes in patients with Latent Autoimmune Diabetes in Adults
Authors: Fu Xiuli , Tan Qin , Wei Wei , Ding Sheng , Wang Zhongjing 
Published: June 2022

Abstract
Aims
The objective of this study is to explore the relationship between red blood cell distribution and islet β-cell function indexes in patients with Latent Autoimmune Diabetes in Adults.
Methods
A total of 487 LADA patients were enrolled in this cross-sectional study. Patients were divided into three groups according to RDW tertiles. Clinical and laboratory measurements of age, height, weight, duration of diabetes, blood pressure, RDW, glycosylated hemoglobin A1c (HbA1c), C-peptide and blood lipids were performed. Homeostasis model assessment of insulin resistance (HOMA-IR) and homeostasis model assessment of β-cell function (HOMA-β) were assessed using homeostasis model assessment (HOMA) based on fasting blood glucose (FBG) and fasting C-peptide index (FCP). Correlations and multiple linear regressions were implemented to determine the association of RDW and islet function indexes.
Results
As the increase of serum RDW level, the presence of β-cell secretion increased(P < 0.05). Correlation analysis indicated that there were significant correlations between RDW and male sex, age, duration, TG, Cr, FCP, and HOMA-β in all subjects. Multiple linear regressions indicated that RDW was significantly correlated with HOMA-β in the total population in both unadjusted and adjusted analysis. This finding could be reproduced in the subgroup of low GAD titers for HOMA-β. RDW were significantly associated with HbA1c in LADA patients with high GAD titers, but the correlation was not found in subgroup with low GAD titers in either unadjusted analyses or adjusted analysis.
Conclusions
RDW is associated with β-cell function assessed by HOMA-β after adjusting for covariates in LADA patients with low GAD titers.
Investigating the Conditions of Automatic Assembly
Authors: Vartanov Mikhail Vladimirovich , Inna Nikolaevna Zinina , Irina Leontievna Klimenko , Tran Dinh Van 
Published: June 2022

Abstract
The paper's purpose is to provide the technological reliability of the robotic assembly of joints with RK profile based on adaptation devices and low-frequency oscillations. Ensuring the assembly conditions are achieved by the vibration device that provides oscillations relative to the two axes, perpendicular to the assembly direction, and rotation about the assembly axis. An adaptive gripper compensates for the linear error in the position parts with a flexible connection. A mathematical model describing the assembly process of parts relative to the non-inertial coordinate system is developed. The technological modes of profile parts assembly are defined. The robotic assembly method of profile joints by the adaptation devices, namely a combination of elastic fixing of the installed profile part and the simultaneous rotation and vibration of the base part to improve the process reliability, is developed. Experimental studies confirmed the adequacy of the created mathematical model. The patent for the assembly method of profile joints with a gap is received.