bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preliminary exploration of the co-regulation of Alzheimer’s disease pathogenic genes by microRNAs and transcription factors
Authors: Zhang Qi , Yang Ping , Pang Xinping , Guo Wenbo , Sun Yue , Wei Yanyu , Pang Chaoyang 
Published: December 2022

BackgroundAlzheimer’s disease (AD) is the most common form of age-related neurodegenerative disease. Unfortunately, due to the complexity of pathological types and clinical heterogeneity of AD, there is a lack of satisfactory treatment for AD. Previous studies have shown that microRNAs and transcription factors can modulate genes associated with AD, but the underlying pathophysiology remains unclear.MethodsThe datasets GSE1297 and GSE5281 were downloaded from the gene expression omnibus (GEO) database and analyzed to obtain the differentially expressed genes (DEGs) through the “R” language “limma” package. The GSE1297 dataset was analyzed by weighted correlation network analysis (WGCNA), and the key gene modules were selected. Next, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis for the key gene modules were performed. Then, the protein-protein interaction (PPI) network was constructed and the hub genes were identified using the STRING database and Cytoscape software. Finally, for the GSE150693 dataset, the “R” package “survivation” was used to integrate the data of survival time, AD transformation status and 35 characteristics, and the key microRNAs (miRNAs) were selected by Cox method. We also performed regression analysis using least absolute shrinkage and selection operator (Lasso)-Cox to construct and validate prognostic features associated with the four key genes using different databases. We also tried to find drugs targeting key genes through DrugBank database.ResultsGO and KEGG enrichment analysis showed that DEGs were mainly enriched in pathways regulating chemical synaptic transmission, glutamatergic synapses and Huntington’s disease. In addition, 10 hub genes were selected from the PPI network by using the algorithm Between Centrality. Then, four core genes (TBP, CDK7, GRM5, and GRIA1) were selected by correlation with clinical information, and the established model had very good prognosis in different databases. Finally, hsa-miR-425-5p and hsa-miR-186-5p were determined by COX regression, AD transformation status and aberrant miRNAs.ConclusionIn conclusion, we tried to construct a network in which miRNAs and transcription factors jointly regulate pathogenic genes, and described the process that abnormal miRNAs and abnormal transcription factors TBP and CDK7 jointly regulate the transcription of AD central genes GRM5 and GRIA1. The insights gained from this study offer the potential AD biomarkers, which may be of assistance to the diagnose and therapy of AD.
Dyslexia and dysgraphia of primary progressive aphasia in Chinese: A systematic review
Authors: Liu Junyan , Ota Shoko , Kawakami Nobuko , Kanno Shigenori , Suzuki Kyoko 
Published: December 2022

IntroductionCurrently, little is known about Chinese-speaking primary progressive aphasia (PPA) patients compared to patients who speak Indo-European languages. We examined the demographics and clinical manifestations, particularly reading and writing characteristics, of Chinese patients with PPA over the last two decades to establish a comprehensive profile and improve diagnosis and care.MethodsWe reviewed the demographic features, clinical manifestations, and radiological features of Chinese-speaking PPA patients from 56 articles published since 1994. We then summarized the specific reading and writing errors of Chinese-speaking patients.ResultsThe average age of onset for Chinese-speaking patients was in their early 60's, and there were slightly more male patients than female patients. The core symptoms and images of Chinese-speaking patients were similar to those of patients who speak Indo-European languages. Reading and writing error patterns differed due to Chinese's distinct tone and orthography. The types of reading errors reported in Chinese-speaking patients with PPA included tonal errors, regularization errors, visually related errors, semantic errors, phonological errors, unrelated errors, and non-response. Among these errors, regularization errors were the most common in semantic variant PPA, and tonal errors were specific to Chinese. Writing errors mainly consisted of non-character errors (stroke, radical/component, visual, pictograph, dyskinetic errors, and spatial errors), phonologically plausible errors, orthographically similar errors, semantic errors, compound word errors, sequence errors, unrelated errors, and non-response.ConclusionThis paper provides the latest comprehensive demographic information and unique presentations on the reading and writing of Chinese-speaking patients with PPA. More detailed studies are needed to address the frequency of errors in reading and writing and their anatomical substrates.
Adjuvant chemotherapy for lymph node positive esophageal squamous cell cancer: The prediction role of low mean platelet volume
Authors: Liu Xiaoling , Zhang Kaijiong , Tang Jie , Jiang Li , Jiang Yu , Wang Qifeng 
Published: December 2022

BackgroundThis study aimed to examine whether MPV is a useful prognostic marker and investigated whether MPV is a risk factor that helps identify patients with locally advanced-stage ESCC who will most likely benefit from adjuvant chemotherapy.MethodsPatients (n =1690) with histologically confirmed ESCC were diagnosed with locally advanced stage (pT3-4N0M0 and pT1-4N+M0) at Sichuan Cancer Hospital from 2009 to 2017. Clinicopathological factors and platelet-related values were tested for their associations with survival using univariate and multivariate Cox regression analyses. The optimal cut-off value for continuous variables was determined using the ‘maxstat’ R package. The KM curve continuous variable analysis was performed to identify the optimal cut-off value for MPV. Cumulative survival rates were determined using the Kaplan–Meier estimator and compared using the log-rank test. The survival analysis was performed using the ‘survival’ R package. All statistical analyses were performed using R software 4.1.3 (https://www.r-project.org/), and a two-sided p-value &lt;0.05 was considered to indicate statistical significance.ResultsMultivariate analysis indicated that low MPV was an important risk factor for overall survival in locally advanced ESCC, independent of classic clinicopathological factors. The optimal cut-off value of MPV (11.8 fL) was used to stratify high-risk patients. Patients with low mean platelet volumes had a worse prognosis than those with larger platelet volumes, according to Kaplan–Meier analysis and the log-rank test. Patients diagnosed with a pathological lymph node-positive stage with a low MPV (≤11.8 fL) benefited from postoperative chemotherapy, but not those with a high-level MPV (&gt;11.8 fL).ConclusionMPV served as an independent predictor of prognosis of locally advanced-stage ESCC and predicted a survival benefit conferred by postoperative adjuvant chemotherapy in lymph node-positive ESCC.
Generic switching: Do future physicians in Jordan have enough knowledge and a positive attitude?
Authors: Al Zoubi Sura , Gharaibeh Lobna , Al-Masri Batool , Alsahele Ahmad B. , AL-Masaeid Buthainah 
Published: December 2022

Background: Generic switching is a policy that has shown success in minimising pharmaceutical costs. It has also been used to mitigate recurrent and sudden drug shortages. Not all countries have policies that allow pharmacists to switch to generic drugs independently. In Jordan, only pharmacists at Ministry of Health hospitals automatically switch to generics if doctors had not already done INN prescribing.Objectives: This study targeted medical students to assess their experience with generic switching as patients, their knowledge of the subject as students, and their attitude towards it as future prescribers and policymakers.Methods: This is a descriptive, cross-sectional study conducted online. Eligibility criteria were being a fourth, fifth, or sixth-year medical school student enrolled at any of the six Jordanian universities. The questionnaire was developed by the researchers after a careful review of the relevant literature.Results: Three hundred and ninety students responded to the online questionnaire. Most participants were females (244, 62.6%), senior students in their final (6th) year (162, 41.5%) and with very good academic achievement (166, 42.6%). The highest knowledge scores concerned patient rights (0.73/1.00), followed by knowledge about monitoring after generic switching (0.66/1.00), and patients with known drug allergies (0.66/1.00). Almost half of the participants believe that pharmacists should not be given the right to do generic switching and only 16% stated that they would choose generic drugs if they needed treatment in the future. Multivariate linear regression analysis showed that significant predictors of knowledge were gender, GPA, and family income. No correlations were found between participants’ knowledge scores and their attitudes towards giving pharmacists the right to independently switch drugs, or whether they would accept a substitute from pharmacists rather than having to refer to the physician.Conclusion: Medical students in Jordan lack sufficient knowledge about generic switching. Students need to be more aware of the current policies and regulations of this practice, and the role of each healthcare worker involved in it. They also need to have a more positive attitude toward generic drugs and generic switching practice to facilitate its future implementation.
Role of miRNAs in regulation of SA-mediated upregulation of genes involved in folate and methionine metabolism in foxtail millet
Authors: Hou Siyu , Men Yihan , Zhang Yijuan , Zhao Kai , Ma Guifang , Li Hongying , Han Yuanhuai , Sun Zhaoxia 
Published: December 2022

The effect of exogenous salicylic acid (SA) on folate metabolism and the related gene regulatory mechanisms is still unclear. In this study, the panicle of foxtail millet treated with different SA concentrations showed that 6 mM SA doubled the 5-methyltetrahydrofolate content compared to that of the control. An untargeted metabolomic analysis revealed that 275 metabolites were enriched in amino acid metabolic pathways. Significantly, the relative content of methionine (Met) after 6 mM SA treatment was 3.14 times higher than the control. Transcriptome analysis revealed that differentially expressed genes were mainly enriched in the folate and amino acid biosynthesis pathways (including Met, Cys, Pro, Ser et al.). The miRNA−mRNA interactions related to the folate and Met metabolic pathways were analyzed and several likely structural gene targets for miRNAs were identified, miRNA-seq analysis revealed that 33 and 51 miRNAs targeted 11 and 15 genes related to the folate and Met pathways, respectively. Eight key genes in the folate metabolism pathway were likely to be up-regulated by 14 new miRNAs and 20 new miRNAs up-regulated the 9 key genes in the Met metabolism pathway. The 6 miRNA−mRNA interactions related to the folate and Met metabolism pathways were verified by qRT−PCR, and consistent with the prediction. The results showed that DHFR1 gene expression level related to folate synthesis was directly up-regulated by Nov-m0139-3p with 3.8 times, but DHFR2 was down-regulated by Nov-m0731-5p with 0.62 times. The expression level of CYSC1 and APIP related to Met synthesis were up-regulated by Nov-m0461-5p and Nov-m0664-3p with 4.27 and 1.32 times, respectively. Our results suggested that exogenous SA could induce the folate and Met accumulated in the panicle of foxtail millet. The higher expression level of DHFR1, FTHFD, CYSC1 and APIP in the folate and Met metabolism pathway and their regulators, including Nov-m0139-3p, Nov-m0717-5p, Nov-m0461-5p and Nov-m0664-3p, could be responsible for these metabolites accumulation. This study lays the theoretical foundation for elucidating the post-transcription regulatory mechanisms of folate and Met metabolism.
The effects of materialism and ego depletion on intertemporal choice: An event-related potential study
Authors: Pei Yingying , Yu Junjian , Zhao Lijun 
Published: December 2022

ObjectiveThe study aims to promote human beings to make scientific and reasonable decisions for the long-term and beautiful future.MethodsWe designed two experiments to explore the influence of materialism and ego depletion from the perspective of behavioral decision-making and neural mechanism.ResultsIn Experiment 1, there was asymmetry in intertemporal choice between gain and loss situations. In the gain situation, high materialism were more likely to choose the later and larger option (LL). However, in a loss situation, we found a reverse sign effect, and the proportion of subjects choosing sooner and smaller options (SS) increased. In Experiment 2, in the gain situation, after adding the low ego depletion task, there was a marginal significant difference between high and low materialism in the percentage of choosing LL options, F(1, 40) = 3.37, P = 0.07, η2 = 0.08; After adding the high ego depletion task, the percentage of choosing LL options was no difference, F(1, 40) = 1.42, P &gt; 0.05. In the loss situation, whether in the high ego depletion task [F(1, 40) = 2.25, P &gt; 0.05) or in the low ego depletion task [F(1, 40) = 1.44, P &gt; 0.05), there was no difference between high and low materialism in the percentage of choosing LL options, and they both tended to choose SS options. The EEG study showed that in high materialism, there was a significant difference between the high and low ego depletion conditions, and the N1 amplitude induced under the low ego depletion condition was larger than that under the high ego depletion condition. However, there was no significant difference in N1 amplitude between the high and low ego depletion conditions in the low materialism. The amplitude of P2 evoked in the loss situation was larger than that in the gain situation.ConclusionIn conclusion, Materialism dominated people’s intertemporal choices, and ego depletion affected the intertemporal choice to a certain extent by influencing the subjects’ thinking activities. The COVID-19 epidemic maybe affected intertemporal choice indirectly by acting on materialistic values and subjects’ emotions.
COVID-19 vaccination motivation and underlying believing processes: A comparison study between individuals with affective disorder and healthy controls
Authors: Dalkner Nina , Fleischmann Eva , Fellendorf Frederike T. , Wagner-Skacel Jolana , Schönthaler Elena M. D. , Bengesser Susanne , Häussl Alfred , Tietz Sophie , Tmava-Berisha Adelina , Lenger Melanie , Reininghaus Eva Z. 
Published: December 2022

BackgroundBelieving processes represent fundamental brain functions between cognition and emotion. Shortly before the introduction of a compulsory vaccination against COVID-19 in Austria, motives and underlying believing processes regarding the vaccination were collected in individuals with affective disorder (AD) and healthy controls (HC).Methods79 individuals with AD and 173 HC were surveyed online to assess believing processes with the parameters of the credition model (narratives, certainty, emotion, mightiness) about (1) the coronavirus itself and (2) why someone is vaccinated or not. In addition, we calculated congruence scores between content of narrative and type of emotion and divided the narrative content into positive, negative, and indifferent.ResultsThere were no differences in vaccination status between AD and HC. Higher levels of certainty were observed in HC compared to AD in both vaccinated and unvaccinated individuals. The effects were higher when asked about the motivation to vaccinate or not than about the coronavirus itself. In HC, more positive emotions and more congruence between emotions and narratives were reported during believing in their vaccination motives. No group differences were found in mightiness for both items. Independently from diagnosis, unvaccinated people had high levels of certainty and more negative emotions and narratives while believing in their motives for not getting vaccinated.ConclusionWhen believing about the COVID-19 vaccination, individuals with AD were more uncertain and experienced fewer positive emotions than HC, although both groups did not differ in vaccination status. These effects were not that strong when believing about the coronavirus in general.
COVI-Prim international: Similarities and discrepancies in the way general practices from seven different countries coped with the COVID-19 pandemic
Authors: Siebenhofer Andrea , Scott Anna Mae , Avian Alexander , Terebessy András , Mergenthal Karola , Schaffler-Schaden Dagmar , Bachler Herbert , Huter Sebastian , Zelko Erika , Murray Amanda , Guppy Michelle , Piccoliori Giuliano , Streit Sven , Jeitler Klaus , Flamm Maria 
Published: December 2022

ObjectivesGeneral practitioners (GPs) are frequently patients' first point of contact with the healthcare system and play an important role in identifying, managing and monitoring cases. This study investigated the experiences of GPs from seven different countries in the early phases of the COVID-19 pandemic.DesignInternational cross-sectional online survey.SettingGeneral practitioners from Australia, Austria, Germany, Hungary, Italy, Slovenia and Switzerland.ParticipantsOverall, 1,642 GPs completed the survey.Main outcome measuresWe focused on how well-prepared GPs were, their self-confidence and concerns, efforts to control the spread of the disease, patient contacts, information flow, testing procedures and protection of staff.ResultsGPs gave high ratings to their self-confidence (7.3, 95% CI 7.1–7.5) and their efforts to control the spread of the disease (7.2, 95% CI 7.0–7.3). A decrease in the number of patient contacts (5.7, 95% CI 5.4–5.9), the perception of risk (5.3 95% CI 4.9–5.6), the provision of information to GPs (4.9, 95% CI 4.6–5.2), their testing of suspected cases (3.7, 95% CI 3.4–3.9) and their preparedness to face a pandemic (mean: 3.5; 95% CI 3.2–3.7) were rated as moderate. GPs gave low ratings to their ability to protect staff (2.2 95% CI 1.9–2.4). Differences were identified in all dimensions except protection of staff, which was consistently low in all surveyed GPs and countries.ConclusionAlthough GPs in the different countries were confronted with the same pandemic, its impact on specific aspects differed. This partly reflected differences in health care systems and experience of recent pandemics. However, it also showed that the development of structured care plans in case of future infectious diseases requires the early involvement of primary care representatives.
Development of an intelligent system based on metaverse learning for students with disabilities
Authors: Sghaier Souhir , Elfakki Abir Osman , Alotaibi Abdullah Alhumaidi 
Published: December 2022

Due to the coronavirus-2019 pandemic, people have had to work and study using the Internet such that the strengthened metaverse has become a part of the lives of people worldwide. The advent of technology linking the real and virtual worlds has facilitated the transmission of spatial audio and haptics to allow the metaverse to offer multisensory experiences in diverse fields, especially in teaching. The main idea of the proposed project is the development of a simple intelligent system for meta-learning. The suggested system should be self-configurable according to the different users of the metaverse. We aimed to design and create a virtual learning environment using Open Simulator based on a 3D virtual environment and simulation of the real-world environment. We then connected this environment to a learning management system (Moodle) through technology for 3D virtual environments (Sloodle) to allow the management of students, especially those with different abilities, and followed up on their activities, tests, and exams. This environment also has the advantage of storing educational content. We evaluated the performance of the Open Simulator in both standalone and grid modes based on the login times. The result showed times the standalone and grid modes of 12 s and 16 s, which demonstrated the robustness of the proposed platform. We also tested the system on 50 disabled learners, according to the t-test of independent samples. A test was conducted in the mathematics course, in which the students were divided into two equal groups (n = 25 each) to take the test traditionally and using the chair test tool, which is one of the most important tools of the Sloodle technology. According to the results, the null hypothesis was rejected, and we accepted the alternative hypothesis that demonstrated a difference in achievement between the two groups.
“Do not look at me like that”: Is the facial expression score reliable and accurate to evaluate pain in large domestic animals? A systematic review
Authors: Fischer-Tenhagen Carola , Meier Jennifer , Pohl Alina 
Published: December 2022

IntroductionFacial expression scoring has proven to be useful for pain evaluation in humans. In the last decade, equivalent scales have been developed for various animal species, including large domestic animals. The research question of this systematic review was as follows: is facial expression scoring (intervention) a valid method to evaluate pain (the outcome) in large domestic animals (population)?MethodWe searched two databases for relevant articles using the search string: “grimace scale” OR “facial expression” AND animal OR “farm animal” NOT “mouse” NOT “rat” NOT “laboratory animal.” The risk of bias was estimated by adapting the Quality Assessment of Diagnostic Accuracy Studies (QUADAS) checklist.ResultsThe search strategy extracted 30 articles, with the major share on equids and a considerable number on cows, pigs, and sheep. Most studies evaluated facial action units (FAUs), including the eye region, the orbital region, the cheek or the chewing muscles, the lips, the mouth, and the position of the ears. Interobserver reliability was tested in 21 studies. Overall FAU reliability was substantial, but there were differences for individual FAUs. The position of the ear had almost perfect interobserver reliability (interclass coefficient (ICC): 0.73–0.97). Validity was tested in five studies with the reported accuracy values ranging from 68.2 to 80.0%.DiscussionThis systematic review revealed that facial expression scores provide an easy method for learning and reliable test results to identify whether an animal is in pain or distress. Many studies lack a reference standard and a true control group. Further research is warranted to evaluate the test accuracy of facial expression scoring as a live pen side test.
Corpo, Subjetividade e Cura no Paradigma Ritual Tântrico
Authors: Costa Lima Danillo 
Published: December 2022

Este ensaio aborda as práticas de cura espiritual na Índia clássica que ainda prevalecem em todo o subcontinente. Baseando-se primariamente no texto Netratantra, com seu escopo de exorcismo religioso, cura mântrica e práticas yoguicas e contemplativas, este ensaio explora a possibilidade de relacionar as práticas concretas com o que foi inscrito nos textos a partir da cultura. Esta pesquisa demonstra a relação fluida existente entre as tradições textuais e orais, em contraste com a ideia de ‘alta’ e ‘baixa’ culturas concebidas pelos indólogos. Os textos tântricos são exemplares nesse respeito, pois tentam fornecer uma estrutura interpretativa para as referências culturais que estão codificando.

Em um sentido mais amplo, este ensaio explora a compreensão do corpo que é pressuposta nas prescrições detalhadas da cura com mantras. Embora o que esteja doente seja a carne, as práticas de cura detalhadas aqui são direcionadas ao corpo construído mentalmente, e os rituais incluem visualização, canto e outras formas de práticas. O corpo neste sistema de crenças desafia as fronteiras opostas de ‘fora’ e ‘dentro’, e ‘sujeito’ e ‘objeto’. O processo de cura depende da interação e interpenetração do corpo mental com o corpo construído e a carne por meio de mantras e outros agentes.