bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing a curriculum to improve cardiology fellows’ training in pregnancy and cardiovascular disease
Authors: Maxner Benjamin , Hansra Barinder , Sibai Diana , Moinul Sheikh , Panella Leslie , Jeha Jeannine , Fiore Catherine , Dumont Tina , Lauring Julianne , Aurigemma Gerard , Harrington Colleen M. , Kovell Lara C. 
Published: March 2022

Abstract
Background
Exposure to pregnant women with cardiovascular disease (CVD) during cardiology fellowship training is limited and without a standard curriculum in the United States. The authors sought to evaluate a dedicated curriculum to teach management of CVD in pregnancy to improve general cardiology fellowship training.

Methods
The authors developed a dedicated CVD in pregnancy curriculum for the general cardiology fellows at a large academic medical center in the fall of 2019. Fellows’ knowledge was assessed via a board-style examination and exposure and attitudes related to the care of pregnant women with CVD were evaluated with a needs assessment questionnaire before and after the curriculum.

Results
Of the 17 fellows who participated in the curriculum, 12 completed the needs assessment pre-curriculum and 9 post-curriculum. The mean (SD) number of pregnant women with CVD cared for by each fellow in the inpatient and outpatient settings were 0.75 (1.29) and 0.56 (0.73), respectively. After the curriculum, all fellows reported awareness of available resources to treat pregnant women with CVD, while a majority disagreed that they receive regular exposure to pregnant patients with CVD in their training. The authors observed significant increases in fellows’ confidence in their knowledge of normal cardiovascular physiology of pregnancy, physical exam skills, and ability to care for pregnant women with valvular disease and arrhythmias from pre to post-curriculum. A total of 15 fellows completed the board-style exam pre-curriculum and 15 post-curriculum. Fellows’ performance on the board-style examination improved slightly from before to after the curriculum (64.0 to 75.3% correct, p = 0.02).

Conclusions
A dedicated curriculum improved cardiology fellows’ knowledge to recognize and treat CVD in pregnancy and improved confidence in caring for this unique patient population.
Tumors carrying BRAF-mutations over-express NAMPT that is genetically amplified and possesses oncogenic properties
Authors: Audrito Valentina , Moiso Enrico , Ugolini Filippo , Messana Vincenzo Gianluca , Brandimarte Lorenzo , Manfredonia Ilaria , Bianchi Simonetta , De Logu Francesco , Nassini Romina , Szumera-Ciećkiewicz Anna , Taverna Daniela , Massi Daniela , Deaglio Silvia 
Published: March 2022

Abstract
Background
Nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme in nicotinamide adenine dinucleotide (NAD) biosynthesis, is up-regulated in several cancers, including metastatic melanoma (MM). The BRAF oncogene is mutated in different cancer types, among which MM and thyroid carcinoma (THCA) are prominent. Drugs targeting mutant BRAF are effective, especially in MM patients, even though resistance rapidly develops. Previous data have linked NAMPT over-expression to the acquisition of BRAF resistance, paving the way for therapeutic strategies targeting the two pathways.

Methods
Exploiting the TCGA database and a collection of MM and THCA tissue microarrays we studied the association between BRAF mutations and NAMPT expression. BRAF wild-type (wt) cell lines were genetically engineered to over-express the BRAF V600E construct to demonstrate a direct relationship between over-activation of the BRAF pathway and NAMPT expression. Responses of different cell line models to NAMPT (i)nhibitors were studied using dose–response proliferation assays. Analysis of NAMPT copy number variation was performed in the TCGA dataset. Lastly, growth and colony forming assays were used to study the tumorigenic functions of NAMPT itself.

Results
The first finding of this work is that tumor samples carrying BRAF-mutations over-express NAMPT, as demonstrated by analyzing the TCGA dataset, and MM and THC tissue microarrays. Importantly, BRAF wt MM and THCA cell lines modified to over-express the BRAF V600E construct up-regulated NAMPT, confirming a transcriptional regulation of NAMPT following BRAF oncogenic signaling activation. Treatment of BRAF-mutated cell lines with two different NAMPTi was followed by significant reduction of tumor growth, indicating NAMPT addiction in these cells. Lastly, we found that several tumors over-expressing the enzyme, display NAMPT gene amplification. Over-expression of NAMPT in BRAF wt MM cell line and in fibroblasts resulted in increased growth capacity, arguing in favor of oncogenic properties of NAMPT.

Conclusions
Overall, the association between BRAF mutations and NAMPT expression identifies a subset of tumors more sensitive to NAMPT inhibition opening the way for novel combination therapies including NAMPTi with BRAFi/MEKi, to postpone and/or overcome drug resistance. Lastly, the over-expression of NAMPT in several tumors could be a key and broad event in tumorigenesis, substantiated by the finding of NAMPT gene amplification.
Seasonal movements in caribou ecotypes of Western Canada
Authors: Theoret Jessica , Cavedon Maria , Hegel Troy , Hervieux Dave , Schwantje Helen , Steenweg Robin , Watters Megan , Musiani Marco 
Published: March 2022

AbstractBackgroundSeveral migratory ungulates, including caribou, are dramatically declining. Caribou of the Barren-ground ecotype, which forms its own subspecies, are known to be mainly migratory. By contrast, within the Woodland subspecies, animals of the Boreal ecotype are known to be mainly sedentary, while those within the Northern and Central Mountain ecotypes to be partially migratory, with only some individuals migrating. Promotion of conservation actions (e.g., habitat protection) that are specific to both residents and migrants, as well as to the areas they frequent seasonally (which may be separate for migrants), requires distinguishing migration from other movement behaviours, which might be a challenge.MethodsWe aimed at assessing seasonal movement behaviours, including migratory, resident, dispersing, and nomadic, for caribou belonging to the Barren-ground and Woodland subspecies and ecotypes. We examined seasonal displacement, both planar and altitudinal, and seasonal ranges overlap for 366 individuals that were GPS-collared in Northern and Western Canada. Lastly, we assessed the ability of caribou individuals to switch between migratory and non-migratory movement behaviours between years.ResultsWe detected migratory behaviour within each of the studied subspecies and ecotypes. However, seasonal ranges overlap (an index of sedentary behaviour) varied, with proportions of clear migrants (0 overlap) of 40.94% for Barren-ground caribou and 23.34% for Woodland caribou, and of 32.95%, 54.87%, and 8.86% for its Northern Mountain, Central Mountain, and Boreal ecotype, respectively. Plastic switches of individuals were also detected between migratory, resident, dispersing, and nomadic seasonal movements performed across years.ConclusionsOur unexpected findings of marked seasonal movement plasticity in caribou indicate that this phenomenon should be better studied to understand the resilience of this endangered species to habitat and climatic changes. Our results that a substantial proportion of individuals engaged in seasonal migration in all studied ecotypes indicate that caribou conservation plans should account for critical habitat in both summer and winter ranges. Accordingly, conservation strategies are being devised for the Woodland subspecies and its ecotypes, which were found to be at least partially migratory in this study. Our findings that migration is detectable with both planar and altitudinal analyses of seasonal displacement provide a tool to better define seasonal ranges, also in mountainous and hilly environments, and protect habitat there.
Debugging behaviors of early childhood teacher candidates with or without scaffolding
Authors: Kim ChanMin , Vasconcelos Lucas , Belland Brian R. , Umutlu Duygu , Gleasman Cory 
Published: March 2022

AbstractIt is critical to teach all learners to program and think through programming. But to do so requires that early childhood teacher candidates learn to teach computer science. This in turn requires novel pedagogy that can both help such teachers learn the needed skills, but also provide a model for their future teaching. In this study, we examined how early childhood teacher candidates learned to program and debug block-based code with and without scaffolding. We aimed to see how approaches to debugging vary between early childhood teacher candidates who were provided debugging scaffolds during block-based programming and those who were not. This qualitative case study focused on 13 undergraduates majoring in early childhood education. Data sources included video recording during debugging, semi-structured interviews, and (in the case of those who used scaffolding) scaffold responses. Research team members coded data independently and then came to consensus. With hypothesis-driven scaffolds, participants persisted longer. Use of scaffolds enabled the instructor to allow struggle without immediate help for participants. Collaborative reasoning was observed among the scaffolded participants whereas the participants without scaffolds often debugged alone. Regardless of scaffolds, participants often engaged in embodied debugging and also used trial and error. This study provides evidence that one can find success debugging even when engaging in trial and error. This implies that attempting to prevent trial and error may be counterproductive in some contexts. Rather, computer science educators may be advised to promote productive struggle.
Improved voltage tracking of autonomous microgrid technology using a combined resonant controller with lead-lag compensator adopting negative imaginary theorem
Authors: Haque Md. Yah-Ya Ul , Islam Md. Rashidul , Ahmed Tanvir , Sheikh Md. Rafiqul Islam 
Published: March 2022

AbstractGrowing application of distributed generation units at remote places has led to the evolution of microgrid (MG) technology. When an MG system functions independently, i.e., in autonomous mode, unpredictable loads and uncertainties emerge throughout the system. To obtain stable and flexible operation of an autonomous MG, a rigid control mechanism is needed. In this paper, a robust high-performance controller is introduced to improve the performance of voltage tracking of an MG system and to eliminate stability problems. A combination of a resonant controller and a lead-lag compensator in a positive position feedback path is designed, one which obeys the negative imaginary (NI) theorem, for both single-phase and three-phase autonomous MG systems. The controller has excellent tracking performance. This is investigated through considering various uncertainties with different load dynamics. The feasibility and effectiveness of the controller are also determined with a comparative analysis with some well-known controllers, such as linear quadratic regulator, model predictive and NI approached resonant controllers. This confirms the superiority of the designed controller.
Evaluation of Turkey’s contribution to SCI-E indexed publications on COVID-19
Authors: Yılmaz Fulya , Bas Koray 
Published: March 2022

Abstract
Background
In December of 2019, a new disease which is caused by SARS-CoV-2, as an epidemic disease out of Wuhan, China, began to circulate. On March 11, 2020, the Republic of Turkey Ministry of Health had announced the first case from Turkey. The aim of this study is to analyze the scientific publications in the field of COVID-19 included in Science Citation Index Expanded (SCIE) from Turkey and to establish a theoretical background for future studies in the health literature with obtained valuable information about the publications. We searched all papers published in the field of COVID-19 by using the terms of “COVID-19,” “2019-n-CoV,” “SARS-CoV-2,” “Coronavirus disease 19,” and “2019 novel coronavirus” as scientific nomenclatures of COVID-19 in the topic search section of the software.

Results
Overall, 47,368 papers, indexed by SCI-E, were found related to COVID-19 between January 1, 2020, and December 13, 2020. Of these, 931 were from Turkey. In terms of specialities, the most contribution was from the Medicine General Internal followed by Dermatology. Most of the publications were article. English was the most preferred language in papers. Dermatological Theraphy published the most paper.

Conclusions
Applying this kind of analysis on an intermittent basis gives a general perspective for contribution of a countries to scientific publications and useful for the further studies.
Sucrose- and formaldehyde-modified native starches as possible pharmaceutical excipients in tableting
Authors: Okeke Ifeanyi Justin , Oli Angus Nnamdi , Ojiako Chioma Miracle , Ibezim Emmanuel Chinedum , Okoyeh Jude N. 
Published: March 2022

Abstract
Background
Starches have been shown to be important across various disciplines such as the pharmaceutical industries, food industries and also paper industries. Starch is basically a mixture of polymers consisting of α-d-glucose as the monomeric unit. The goal of this study is to modify the native starches which were obtained from Zea mays, Triticumestivum and Oriza sativa through cross-linking (using sucrose and formaldehyde at different concentrations) and also to assess the utilizability of the modified starches as potential excipients [binder] for tableting of paracetamol.

Results
Maize and rice starches cross-linked with 2.5% sucrose gave the least percentage moisture content. The batches cross-linked with 40% formaldehyde showed the highest moisture content. The densities (bulk and tapped) of maize wheat and rice starches showed a reduction with the increasing concentration of the cross-linking agent for sucrose, which is the reverse case for formaldehyde. The different concentrations of sucrose and formaldehyde cross-maize, wheat and rice starches had pH values between 4.50 and 5.52. The onset and end set of the glass transition temperatures were varied for all the starches modified with formaldehyde. The melting peak temperatures obtained indicated that the formaldehyde-modified rice starch had significantly lower melting temperature than those of wheat and maize starches.

Conclusions
This study reveals that various concentrations of sucrose and formaldehyde had some influence on the properties of the native starches and resulted in the production of new starch motifs with improved or new functionalities suitable for use as drug excipients in tableting.
Evaluation of the flexion gap with a distal femoral trial component in posterior-stabilized total knee arthroplasty
Authors: Kamei Goki , Ishibashi Shigeki , Yoshioka Koki , Sakurai Satoru , Inoue Hiroyuki , Mochizuki Yu , Ishikawa Masakazu , Adachi Nobuo 
Published: March 2022

Abstract
Purpose
A distal femoral trial component was manufactured, and flexion gap size and inclination were evaluated with or without the distal femoral trial component in total knee arthroplasty (TKA). This study aimed to evaluate the effect of the distal femoral trial component on flexion gap size and joint inclination in posterior-stabilized (PS)-TKA.

Materials and methods
A total of 84 patients with medial osteoarthritis who underwent mobile-bearing PS-TKA using modified gap techniques were included in this retrospective study. The flexion gap size and inclination before and after setting the distal femoral trial component were evaluated and compared with the final gap size and inclination.

Results
The joint gap size and inclination were significantly lower in those with than in those without the distal femoral trial component (P = 0.005, P < 0.001). The final gap size and inclination were similar to the gap size and inclination with the distal trial component (P = 0.468, P = 0.158).

Conclusions
The joint gap size and medial tension in PS-TKA were significantly reduced after setting the distal femoral trial component. The flexion gap measured using the distal femoral trial component was similar to that when the final trial component was set. To more accurately perform the gap technique TKA, the flexion gap should be measured using the distal femoral trial component.
An investigation of 6-Shogaol effects on MCF7 cell lines through a systems biology approach
Authors: Amjad Elham , Sokouti Babak , Asnaashari Solmaz 
Published: March 2022

Abstract
Introduction
In the literature, to investigate hormonal mechanisms of cell growth of patients with breast cancer (BC), as the second most common cause of death in the world, the researchers frequently used MCF-7 cell lines. And, identifying the functional mechanisms of therapeutics agents as new cancer inhibitors is still unclear.

Methods
We used the NCBI-GEO dataset (GSE36973) to study the effects of 6-Shogaol on MCF-7 cell lines commonly used for more than 45 years in several studies. The pre-processing and post-processing stages were carried out for the target samples to identify the most significant differentially expressed genes between two MCF-7 with and without treated by 6-Shogaol. Furthermore, various analyses, including biological process and molecular function from the DAVID website, the protein–protein interaction (PPI) network, gene-miRNA, gene-transcription factor, gene-drugs, and gene-diseases networks, statistically significant assoications with clinical features and survival rates were conducted.

Results
The initial outcomes revealed thirty significant DEGs. Among which the approach resulted in eleven upregulated and nineteen downregulated genes. Over-expression of TRADD and CREB3L1 and low-expression of KIF4A and PALMD were substantial in the TNF signaling pathway. Moreover, hsa-mir-16-5p and hsa-mir-124-3p were inhibitors of breast cancer growth.

Conclusion
The fact that some of genes are associated with survival rates as well as various clinical features including disease stages, it can be deduced that the 6-Shogaol treatment on MCF7 cell lines at the genome level shows inhibition functionalities of the herbal medicine in breast cancer at early stages and pave the way in developing new therapeutic agents.
Radiological approach to non-compressive myelopathies
Authors: Swarup M. Sarthak , Chandola Stuti , Batra Radhika , Prakash Anjali , Garg Anju 
Published: March 2022

Abstract
Background
Myelopathy, a pathological condition related to the spinal cord can broadly be categorized into compressive and non-compressive aetiologies. Magnetic resonance imaging remains the modality of choice when suspecting non-compressive myelopathy as it helps to localize the affected segment and exclude compression as the cause of myelopathy. This review deals with the imaging approach for non-compressive myelopathies.

Main body
Demyelinating disorders are the most common cause of non-compressive myelopathy and often show confounding features. Other causes include inflammatory, ischemic, metabolic, and neoplastic disorders. Non-compressive myelopathy can broadly be classified into acute and non-acute onset which can further be categorized according to the distribution of the signal abnormalities, including length of cord involvement, specific tract involvement, enhancement pattern, and the region of the spinal cord that is affected.

Conclusions
Imaging plays a critical role in the evaluation of clinically suspected cases of myelopathy and MR imaging (with or without contrast) remains the preferred modality. Compressive causes must be excluded as a cause of myelopathy. Despite a multitude of causes, the most common imaging appearance is a nonspecific T2 hyperintense signal in the spinal cord, and thus, a pragmatic diagnostic approach along with appropriate clinical and biochemical correlation is essential for arriving at an accurate diagnosis.
A Case of Methimazole-Induced Acute Pancreatitis With an HLA Allele Causing Antithyroid Drug-Induced Agranulocytosis
Authors: Yoshimura Yusuke , Tatsushima Keita , Goshima Yukiko , Hoshino Yoshitomo , Nakashima Saki , Inaba Tatsuro , Ikeda Sara , Hattori Daisuke , Koyama Rikako , Imamura Tsunao , Takeshita Akira , Takeuchi Yasuhiro 
Published: March 2022

Abstract
Among the side effects of methimazole (MMI) for the treatment of Graves’ disease, MMI-induced acute pancreatitis (MIP) is a rare adverse reaction, with only 7 cases being reported to date. However, 2 large-scale population-based studies recently revealed that the risk of MIP was significantly higher, ranging from 0.02% to 0.56%. Although MIP is common in middle-aged and elderly Asian women, its pathogenesis remains largely unknown. We herein present a case of a 72-year-old Japanese woman with Graves’ disease who developed MIP 12 days after the initiation of MMI. The MMI was discontinued, the patient was switched to propylthiouracil (PTU) therapy, and pancreatitis gradually resolved. Serological human leukocyte antigen (HLA) typing identified HLA-DRB1*08:03:02. This HLA allele was previously detected in a patient with MIP and is one of the major risk factors for agranulocytosis induced by antithyroid drugs, including PTU as well as MMI. In cases of MIP, PTU is being considered as an alternative to MMI; however, its safety needs further investigation and patients require close monitoring after the switch to PTU. Further studies are warranted, particularly on the relationship between MIP and the presence of HLA alleles causing antithyroid drug-induced agranulocytosis.
Diagnostic accuracy of a commercially available deep-learning algorithm in supine chest radiographs following trauma
Authors: Gipson Jacob , Tang Victor , Seah Jarrel , Kavnoudias Helen , Zia Adil , Lee Robin , Mitra Biswadev , Clements Warren 
Published: March 2022

Objectives:
Trauma chest radiographs may contain subtle and time-critical pathology. Artificial intelligence (AI) may aid in accurate reporting, timely identification and worklist prioritisation. However, few AI programs have been externally validated. This study aimed to evaluate the performance of a commercially available deep convolutional neural network – Annalise CXR V1.2 (Annalise.ai) – for detection of traumatic injuries on supine chest radiographs.

Methods:
Chest radiographs with a CT performed within 24 h in the setting of trauma were retrospectively identified at a level one adult trauma centre between January 2009 and June 2019. Annalise.ai assessment of the chest radiograph was compared to the radiologist report of the chest radiograph. Contemporaneous CT report was taken as the ground truth. Agreement with CT was measured using Cohen’s κ and sensitivity/specificity for both AI and radiologists were calculated.

Results:
There were 1404 cases identified with a median age of 52 (IQR 33–69) years, 949 males. AI demonstrated superior performance compared to radiologists in identifying pneumothorax (p = 0.007) and segmental collapse (p = 0.012) on chest radiograph. Radiologists performed better than AI for clavicle fracture (p = 0.002), humerus fracture (p &lt; 0.0015) and scapula fracture (p = 0.014). No statistical difference was found for identification of rib fractures and pneumomediastinum.

Conclusion:
The evaluated AI performed comparably to radiologists in interpreting chest radiographs. Further evaluation of this AI program has the potential to enable it to be safely incorporated in clinical processes.

Advances in knowledge:
Clinically useful AI programs represent promising decision support tools.