bluuebunny/crossref_metadata_2025_split huggingface dataset:
An international report on bacterial communities in esophageal squamous cell carcinoma
Authors: Nomburg Jason , Bullman Susan , Nasrollahzadeh Dariush , Collisson Eric A. , Abedi‐Ardekani Behnoush , Akoko Larry O. , Atkins Joshua R. , Buckle Geoffrey C. , Gopal Satish , Hu Nan , Kaimila Bongani , Khoshnia Masoud , Malekzadeh Reza , Menya Diana , Mmbaga Blandina T. , Moody Sarah , Mulima Gift , Mushi Beatrice P. , Mwaiselage Julius , Mwanga Ally , Newton Yulia , Ng Dianna L. , Radenbaugh Amie , Rwakatema Deogratias S. , Selekwa Msiba , Schüz Joachim , Taylor Philip R. , Vaske Charles , Goldstein Alisa , Stratton Michael R. , McCormack Valerie , Brennan Paul , DeCaprio James A. , Meyerson Matthew , Mmbaga Elia J. , Van Loon Katherine 
Published: July 2022

AbstractThe incidence of esophageal squamous cell carcinoma (ESCC) is disproportionately high in the eastern corridor of Africa and parts of Asia. Emerging research has identified a potential association between poor oral health and ESCC. One possible link between poor oral health and ESCC involves the alteration of the microbiome. We performed an integrated analysis of four independent sequencing efforts of ESCC tumors from patients from high‐ and low‐incidence regions of the world. Using whole genome sequencing (WGS) and RNA sequencing (RNAseq) of ESCC tumors from 61 patients in Tanzania, we identified a community of bacteria, including members of the genera Fusobacterium, Selenomonas, Prevotella, Streptococcus, Porphyromonas, Veillonella and Campylobacter, present at high abundance in ESCC tumors. We then characterized the microbiome of 238 ESCC tumor specimens collected in two additional independent sequencing efforts consisting of patients from other high‐ESCC incidence regions (Tanzania, Malawi, Kenya, Iran, China). This analysis revealed similar ESCC‐associated bacterial communities in these cancers. Because these genera are traditionally considered members of the oral microbiota, we next explored whether there was a relationship between the synchronous saliva and tumor microbiomes of ESCC patients in Tanzania. Comparative analyses revealed that paired saliva and tumor microbiomes were significantly similar with a specific enrichment of Fusobacterium and Prevotella in the tumor microbiome. Together, these data indicate that cancer‐associated oral bacteria are associated with ESCC tumors at the time of diagnosis and support a model in which oral bacteria are present in high abundance in both saliva and tumors of some ESCC patients.
Multi‐center evaluation of the highly sensitive Abbott ARCHITECT and Alinity thyroglobulin chemiluminescent microparticle immunoassay
Authors: Evans Carol , Lotz Johannes , Bhandari Maria , Hellier Rowan T. , Wang Xiao Yan , Lott Rosemarie , Lackner Karl J. , Müller Robert , Kulasingam Vathany 
Published: July 2022

AbstractBackgroundThyroglobulin (Tg) is an essential part for the management of patients with differentiated thyroid carcinoma (DTC) after thyroidectomy. Highly sensitive Tg assays are now established in clinical practice as they facilitate follow‐up of DTC patients. In this study, we evaluated the recently launched highly sensitive Abbott Tg assay for Alinity and ARCHITECT.MethodsIn this three‐center study, Tg values of 447 routine patient samples, characterized for the presence of anti‐Tg, were measured with the ARCHITECT Tg assay and compared with the Roche Elecsys TgII assay. In addition, a subset of 154 samples was compared also with the Beckman Tg Access assay and another subset (n = 122) with Abbott Tg on the Alinity i analyzer.ResultsLoQ was verified to be less than or equal to 0.1 ng/ml confirming that the Tg assay on ARCHITECT and Alinity is highly sensitive. Correlation of ARCHITECT, Alinity, and Roche was excellent with a slope between 0.9 and 1.1 and a correlation coefficient >0.98. Correlation to Beckmann Tg was also very good, but the differences in absolute values were significant (slope: 1.477).ConclusionsThe Abbott Thyroglobulin assay, which is standardized to CRM‐457, demonstrated a high correlation to the Roche and Beckman Tg assays, though good agreement of absolute values was only observed between Abbott and Roche. Strength of correlation and slope were not affected by the presence of anti‐Tg indicating that all assays included in the study have a similar susceptibility to anti‐Tg.
Cumulative risk of developing a new symptom in patients with primary biliary cholangitis and its impact on prognosis
Authors: Kimura Naruhiro , Setsu Toru , Arao Yoshihisa , Sakai Norihiro , Watanabe Yusuke , Abe Hiroyuki , Kamimura Hiroteru , Sakamaki Akira , Yokoo Takeshi , Kamimura Kenya , Tsuchiya Atsunori , Osaki Akihiko , Igarashi Kentarou , Waguri Nobuo , Yanagi Masahiko , Takahashi Toru , Sugitani Soichi , Kobayashi Yuka , Takamura Masaaki , Yoshikawa Akira , Ishikawa Toru , Yoshida Toshiaki , Watanabe Toshiaki , Bannai Hitoshi , Kubota Tomoyuki , Funakoshi Kazuhiro , Wakabayashi Hiroto , Kurita So , Ogata Norio , Watanabe Masashi , Mita Yuhsaku , Mori Shigeki , Sugiyama Motoya , Miyajima Toru , Takahashi Sumio , Sato Shuichi , Ishizuka Kisei , Ohta Hironobu , Aoyagi Yutaka , Terai Shuji 
Published: July 2022

AbstractBackground and AimSymptoms of primary biliary cholangitis (PBC) frequently impair one's quality of life (QOL). Nonetheless, with improved treatment, the prognosis of PBC also improves. QOL plays an important role in patients with PBC. In this study, we aimed to reevaluate the transition of new symptom development in PBC and its predictive factors.MethodsThis retrospective multicenter study enrolled 382 patients with PBC for symptom analysis. The impact of a newly developed symptom on PBC prognosis was investigated by Kaplan–Meier analysis with propensity score matching and logistic progression analysis.ResultsThe cumulative risk of developing a new symptom after 10 and 20 years of follow‐up was 7.6 and 28.2%, and specifically that of pruritus, which was the most common symptom, was 6.7 and 23.3%, respectively. In Cox hazard risk analysis, serum Alb level (hazard ratio [HR], 1.097; 95% confidence interval [CI], 1.033–1.165; P = 0.002), the serum D‐Bil level (HR, 6.262; 95% CI, 2.522–15.553, P < 0.001), and Paris II criteria (HR, 0.435; 95% CI, 0.183–1.036; P = 0.037) were significant independent predictors of a new symptom. Kaplan–Meier analysis showed that the overall survival and liver‐related death were not significant between patients with and without a new symptom.ConclusionThe cumulative risk of new symptom development is roughly 30% 20 years after diagnosis and could be predicted by factors including serum albumin levels, serum D‐Bil level, and Paris II criteria.
What is the effect of a low literacy talking book on patient knowledge, anxiety and communication before radiation therapy starts? A pilot study
Authors: Smith‐Lickess Sian K , Stefanic Natalie , Shaw Joanne , Shepherd Heather , Naehrig Diana , Turner Robin M , Cabrera‐Aguas Maria , Meiser Bettina , Halkett Georgia KB , Jackson Michael , Saade George , Bucci Joseph , Milross Christopher , Dhillon Haryana M 
Published: July 2022

AbstractIntroductionRadiation therapy is a common cancer treatment, requiring timely information to help patients prepare for treatment. We pilot tested a low literacy, psycho‐educational talking book (written booklet, with accompanying audio recording) to examine (i) the effect of the tool on knowledge, anxiety and communication; (ii) acceptability, and (iii) how it was used in appointments.MethodsA pre‐post design was employed. Patients scheduled to receive radiation therapy for any cancer were recruited from two hospitals in Sydney, Australia. Participants were sent the talking book before treatment planning and completed baseline and follow‐up surveys, before and after the intervention.ResultsForty participants were recruited, and 39 completed all study assessments. Overall, knowledge increased after receiving the talking book by 3.8 points from 13.9 to 17.7/20 (95% confidence interval (CI) 2.7, 4.8, P < 0.001). Anxiety and concerns were significantly lower after receiving the talking book (P = 0.015 and P = 0.004, respectively). Nearly half of participants (s = 17, 48%) reported using the book during appointments. Most reported finding it easier to communicate (n = 31, 89%) and to ask more questions (n = 21, 62%).ConclusionThe talking book shows promise in improving knowledge, reducing anxiety and enhancing communication. Strategies to support the implementation of the talking book are required. Further studies to translate the book into different languages are also planned.
Patient reported outcomes: Financial toxicity is a barrier to clinical trials and personalized therapy in cholangiocarcinoma
Authors: Keilson Jessica M. , Lindsey Stacie , Bachini Melinda , Medin Caroline R. , Berk Alexandra , Cornew Sophia , Maithel Shishir K. 
Published: July 2022

AbstractPurposeNumerous experimental and targeted therapies are under investigation for patients with cholangiocarcinoma (CCA). Objective health‐related quality of life (HRQoL) data for patients receiving these therapies are limited.MethodsPatients engaged in the Cholangiocarcinoma Foundation completed two validated HRQoL surveys: Functional Assessment of Cancer Therapy (FACT)‐Hepatobiliary and COmprehensive Score for financial Toxicity (COST).ResultsTwo hundred eight patients were included. Seventy‐five percent had intrahepatic CCA and 57% underwent resection, of which 48% had disease recurrence. Twenty‐two percent enrolled in a clinical trial and 80% underwent molecular profiling, of which 29% received targeted therapy. While patients enrolled in a clinical trial or received targeted therapy reported similar HRQoL compared to those who did not, they reported higher financial toxicity (p = 0.05 and p = 0.01, respectively).ConclusionEnrollment in a clinical trial or receipt of targeted therapy do not affect a patient's physical, emotional, social, or functional well‐being. However, patients report higher financial burden. These therapies are mainly offered in the advanced setting after significant financial strain has been endured and are often only available at large academic centers, creating a physical barrier to access. These findings underscore the need to increase availability and eliminate physical and financial barriers that threaten access and utilization of personalized and progressive therapies.
Product design, social exclusion, and product preference: The mediating role of psychological ownership and the moderating role of product type
Authors: Liu Fu , Wei Haiying , Chen Siyun , Chen Haipeng (Allan) 
Published: July 2022

AbstractThis paper examines the effect of social exclusion and product design philosophy on consumers' design preference, its underlying mechanism, and boundary condition. We propose that rejected (vs. ignored) consumers should prefer a professional‐ (vs. user‐) designed product through increased psychological ownership. Additionally, product complexity should moderate this effect, such that rejected (vs. ignored) consumers should prefer professional‐designed products for low complexity products; for high‐complexity products, we predict a preference for professional‐designed products, regardless of consumers' state of social exclusion. Across four studies (including one using an incentive‐compatible design), we empirically test these hypotheses using different operationalizations of social exclusion. Our research contributes to the literature on product design philosophy, social exclusion, and psychological ownership. The findings offer novel insights regarding ways for marketers to develop marketing strategies to effectively choose and advertise firms' design philosophy.
Current landscape of gene‐editing technology in biomedicine: Applications, advantages, challenges, and perspectives
Authors: Zhou Weilin , Yang Jinrong , Zhang Yalan , Hu Xiaoyi , Wang Wei 
Published: July 2022

AbstractThe expanding genome editing toolbox has revolutionized life science research ranging from the bench to the bedside. These “molecular scissors” have offered us unprecedented abilities to manipulate nucleic acid sequences precisely in living cells from diverse species. Continued advances in genome editing exponentially broaden our knowledge of human genetics, epigenetics, molecular biology, and pathology. Currently, gene editing‐mediated therapies have led to impressive responses in patients with hematological diseases, including sickle cell disease and thalassemia. With the discovery of more efficient, precise and sophisticated gene‐editing tools, more therapeutic gene‐editing approaches will enter the clinic to treat various diseases, such as acquired immunodeficiency sydrome (AIDS), hematologic malignancies, and even severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. These initial successes have spurred the further innovation and development of gene‐editing technology. In this review, we will introduce the architecture and mechanism of the current gene‐editing tools, including clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR‐associated nuclease‐based tools and other protein‐based DNA targeting systems, and we summarize the meaningful applications of diverse technologies in preclinical studies, focusing on the establishment of disease models and diagnostic techniques. Finally, we provide a comprehensive overview of clinical information using gene‐editing therapeutics for treating various human diseases and emphasize the opportunities and challenges.
Flow through fibula and fasciocutaneous free flaps in oromandibular reconstruction
Authors: Gur Ersin , Tiftikcioglu Yigit Ozer , Ozturk Kerem 
Published: July 2022

AbstractIntroductionThe osteocutaneous fibula is a workhorse flap for oromandibular reconstruction. Skin paddles not only perform soft tissue reconstruction but also serve as a monitor for the fibula. In cases where the skin paddle cannot be harvested as desired due to variations, two challenges arise, such as fibula follow‐up and the need for a second free flap so recipient. Moreover, there may not be enough recipient vessels for the double flaps in the neck. This report aimed to address the difficulties mentioned above with the use of flow‐through free flaps in composite oromandibular reconstructions.Patients and MethodsBetween 2019 and 2021, five (three Female, two Male) patients underwent flow‐through technique as free fibula and fasciocutaneous flaps due to variations in fibular skin paddle or insufficiency of recipient vessels in the neck. Ages of patients were between 45 and 75 years. Four patients underwent surgery for tumor and one patient for the result of radionecrosis. ALT, chimeric ALT, and RFFF were selected as second free flaps.ResultsThe size of the fasciocutaneous flaps ranged from 6 × 4 cm to 14 × 11 cm. Mandibular defects ranged from 6 to 16 cm. 1 venous occlusion occurred post‐op 1st day and was salvaged. One hematoma and one wound dehiscence occurred postoperatively and were salvaged successfully. One Partial tongue necrosis occurred due to previous radiotherapy and additional tumor surgery. No additional complication occurred. All flaps survived. Follow‐up period was between 3 months and 2 years. Patient who had tongue necrosis experienced swallowing and speech difficulty and Percutaneous endoscopic gastrostomy tube was placed post‐operative 2 months. Functional finale outcomes were successful for other patients.ConclusionFlow‐through technique provides fibula monitoring with avoiding to find second recipient. Customizing free flaps under more favorable conditions as on the operation table before fixation of the bone can be a useful approach.
Structural Habitat in Lakes and Reservoirs: Physical and Biological Considerations for Implementation
Authors: Sass Greg G. , Shaw Stephanie L. , Fenstermacher Carly C. , Porreca Anthony P. , Parkos Joseph J. 
Published: July 2022

Abstract
In response to declines in coarse woody habitat (CWH) and fish productivity in natural lakes and reservoirs, agencies and stakeholders have used woody and artificial habitat enhancements to slow or reverse the effects of habitat loss from aging or shoreline development. Given that natural lakes and reservoirs differ in physical and biological conditions that could influence habitat enhancement outcomes, a framework is needed to guide management expectations for CWH replacement under different ecosystem contexts. We review ecosystem contexts that influence the effects of structural habitat enhancements in natural lakes and reservoirs, use preliminary results from case studies in a natural lake in Wisconsin and two reservoirs in Illinois to illustrate the importance of these contexts, and provide management recommendations for habitat deployments that consider physical and biological ecosystem characteristics. Because of their influence on trophic transfer efficiency, trophic status and turbidity are important contexts for habitat enhancements in natural lakes and reservoirs. Habitat enhancements to large reservoirs must also contend with high nutrient loading, water level fluctuations, and longitudinal gradients in physical and biological conditions. Preliminary results from the Wisconsin experiment illustrated the importance of the recipient fish community, with rates of response to the structural enhancement varying among fish species. The Illinois case studies demonstrated how the magnitude of change (i.e., effect size) after habitat enhancement can differ (1) between CWH additions to an oligotrophic natural lake and a eutrophic, turbid reservoir and (2) by longitudinal position of artificial habitat within a large reservoir. The functions or ecosystem services of CWH targeted for rehabilitation provide guidance as to which ecosystem features will shape the strength, direction, and duration of response. Future whole-ecosystem manipulations are needed across a wider range of environmental contexts and fish productivity responses, and the strength of productivity increases should be compared to increased harvest efficiency.
A phosphaphenanthrene‐based derivative for simultaneously improving flame retardant and smoke suppression of epoxy resin composites
Authors: Wang Yadong , Yuan Jun , Wang Hao , Ma Li , Lin Xuebao , Deng Hua , Zhu Zongmin 
Published: July 2022

AbstractEpoxy resin (EP) have the disadvantage of high flammability and large amount of smoke, which greatly restricts its wide application in basic material. In order to obtain EP with excellent smoke inhibition and flame retardance, the flame retardant called as PBO, which with both condensed phase and gas phase flame‐retardant effects, was synthesized by the reaction among p‐phenylenediamine, DOPO, and indole‐3‐carboxaldehyde. From combustion results, EP containing PBO obtained V‐0 grade with a significantly improvement from 22.5% to 30.2% in limiting oxygen index. In comparison with EP, total smoke production, total heat release and the peak of heat release of EP/PBO‐1.0 were declined by 32%, 41.5%, and 37.4%, respectively. Besides,Tgof EP composites also had a certain degree of improvement, which due to the increase of rigidity and crosslinking density of EP caused by the addition of PBO. Meanwhile, the analysis of chars and pyrolysis products of PBO presented that the improvement was assigned to two phase synergy through barrier effect of chars in condensed phase and interrupt chain reaction in gaseous phase.
Zinc‐trimesic acid metal–organic framework incorporated sulfonated poly(ether ether sulfone) based polymer composite membranes for fuel cell
Authors: Kulasekaran Poonkuzhali , Maria Mahimai Berlina , Sivasubramanian Gandhimathi , Pushparaj Hemalatha , Deivanayagam Paradesi 
Published: July 2022

AbstractPolymer composite membrane composed of zinc‐trimesic acid based metal–organic framework (ZT‐MOF) and sulfonated poly(ether ether sulfone) (SPEES) for proton exchange membrane fuel cell has been fabricated by solution casting technique. Poly(ether ether sulfone) was sulfonated using concentrated sulfuric acid as a sulfonating agent and the degree of sulfonation was calculated as 36%. The structural analysis of composite membranes was investigated by Fourier transform infrared spectroscopy, scanning electron microscopy, X‐ray diffraction, and X‐ray photoelectron spectroscopy analyses. Essential properties like, ion exchange capacity, proton conductivity, water uptake ability, swelling ratio, thermal stability, contact angle, and oxidative stability of the membranes were studied in detail. The composite membrane loaded with 5% of zinc‐trimesic acid metal–organic framework exhibited the water uptake capacity of 37.18%, whereas the pure membrane was restricted to 27.01%. The ion exchange capacity of the prepared electrolyte membranes possess in the range between 1.53 and 2.80 mEq g−1. The SP/ZT‐MOF‐3 membrane produced the maximum proton conductivity of 0.041 S cm−1, whereas the control sample revealed the ionic conductivity of 0.0125 S cm−1. The composite membrane displayed adequate thermal stability when compared with virgin SPEES polymer. Overall, the experimental data of SP/ZT‐MOF membrane demonstrated that the materials are suitable candidates for fuel cell applications.
Protrusion‐Rich Cu@NiRu Core@shell Nanotubes for Efficient Alkaline Hydrogen Evolution Electrocatalysis
Authors: Liu Xuan , Zhang Siyang , Liang Jiashun , Li Shenzhou , Shi Hao , Liu Jinjia , Wang Tanyuan , Han Jiantao , Li Qing 
Published: July 2022

AbstractThe development of highly efficient and durable water electrolysis catalysts plays an important role in the large‐scale applications of hydrogen energy. In this work, protrusion‐rich Cu@NiRu core@shell nanotubes are prepared by a facile wet chemistry method and used for catalyzing hydrogen evolution reaction (HER) in an alkaline environment. The protrusion‐like RuNi alloy shells with accessible channels and abundant defects possess a large surface area and can optimize the surface electronic structure through the electron transfer from Ni to Ru. Moreover, the unique 1D hollow structure can effectively stabilize RuNi alloy shell through preventing the aggregation of nanoparticles. The synthesized catalyst can achieve a current density of 10 mA cm−2 in 1.0 m KOH with an overpotential of only 22 mV and show excellent stability after 5000 cycles, which is superior to most reported Ru‐based catalysts. Density functional theory calculations illustrate that the weakened hydrogen adsorption on Ru sites induced by the alloying with Ni and active electron transfer between Ru and Ni/Cu are the keys to the much improved HER activity.