bluuebunny/crossref_metadata_2025_split huggingface dataset:
Second Seismic Anchor Point of the Martian Crustal Structure Away From the InSight Landing Site
Authors: Li Jiaqi , Beghein Caroline , McLennan Scott , Horleston Anna , Huang Quancheng , Bozdag Ebru , Golombek Matthew , Lekic Vedran , Lognonné Philippe , Banerdt William 
Published: July 2022

Abstract
The most distant marsquake, S0976a, recorded so far by the InSight seismometer occurred at an epicentral distance of 146.3 ± 6.9o (Horleston et al., 2022), close to the western end of Valles Marineris. We have identified both SS and PP precursors, i.e., underside reflections off the crust-mantle (or intra-crustal) discontinuity between the marsquake and the instrument, on the seismograms, which directly constrain the crustal structure away (4000 km) from the InSight landing site. Inversion results show that the Martian crust at the bounce point between the lander and the event is characterized by an interface at 20 ± 5 km depth, similar to that seen beneath the InSight landing site (Knapmeyer-Endrun et al., 2021). However, a likely distinct feature at the bounce point compared to the lander site is the absence of a shallow layer (at 8 ± 2 km, with low seismic wave speed, Knapmeyer-Endrun et al., 2021), indicating that this layer is either local structure beneath the lander or related to the type of geologic unit or that has been obscured at the bounce point by later processes.
Control of maleic acid-propylene diepoxide hydrogel for 3D printing application for flexible tissue engineering scaffold with high resolution by end capping and graft polymerization
Authors: Tran Hao Nguyen , Kim In Gul , Kim Jong Heon , Chung Eun-Jae , Noh Insup 
Published: July 2022

Abstract
Background
Control of 3D printing of highly tough hydrogel inks with adequate printability, scaffold fidelity and mechanical properties are highly desirable for biomedical and tissue engineering applications. However, developing a biocompatible tough ink with high-resolution printability, biodegradability, adhesion, and integration with surrounding tissues is a big challenge in 3D printing. The aim of this study was to develop extrusion-based 3D printing of viscous hydrogel composing of maleic acid and propylene diepoxide by controlling continuous mechanisms of condensation and radical polymerization.
Methods
The molecular weight of highly adhesive propagating poly(malate-co-propylene oxide) copolymer was controlled by capping its growing chain with mono-functional lipoic acid during condensation reaction to form lipoic acid capped gel (LP-capped gel). Poly(ethylene oxide)-diacrylate, PEGDA, is graft-polymerized to the LP-capped backbone polymer (MPLE gel) by UV irradiation during 3D printing process to control the properties of printability, cell adhesiveness and fidelity with high resolution and mechanical properties (MPLE scaffold). Both the highly adhesive LP-capped gel and printing-controlled MPLE gel/scaffolds are diversely characterized and compared with for their applications to the extrusion-based printability, including biocompatibility, self-healing, drug releasing, adhesiveness, multi-layered high-resolution printing, and in vitro/in vivo tests.
Results
The ratio at 1:0.3 (w/v) between LP-capped gel and PEGDA was chosen for the optimal results for 3D printing at high resolution (90–140µm in strut thickness) with various complex geometries (lattice, rhombus, and honeycomb). In addition, the long-term release profiles of bioactive molecules were well-controlled by incorporating high molecular BSA (21 days, 98.4 ± 0.69%), or small molecule ORN (14 days, 97.1 ± 1.98%) into the MPLE gel scaffolds for the tests of potential therapeutic applications. More importantly, the MPLE gels represents excellent in vitro cyto-compatibility against osteoblast-like cells (MC3T3) with viability value at 96.43% ± 7.48% over 7 culturing days. For in-vivo studies, the flexible MPLE scaffolds showed significant improvement on angiogenesis with minor inflammatory response after 4-week implantation in mice.
Conclusion
The MPLE gel inks was well-controlled for the fabrication of flexible complex tissue engineering scaffold with high resolutions, shear-thinning and post-printing fidelity, by modulating the composition of the highly adhesive LP-capped gel and inert PEGDA as well as end capping of lipoic acid to the propagating poly(malate-co-propylene oxide) copolymer.
Fluorine anion modification of high-voltage Mn-rich phosphate cathodes for high-power and long-lasting sodium-ion batteries
Authors: Zhang Wei , Wu Yulun , Xu Zhenming , Li Jianwei , Dai Yuhang , Zong Wei , Chen Ruwei , Li Shihao , Guo Fei , Li Simin , Wu Feixiang , Zhang Zhian , He Guanjie , Lai Yanqing , Parkin Ivan 
Published: July 2022

Abstract
High-voltage cathodes with high power and stable cyclability are needed for future high-performance sodium-ion batteries. However, the low electronic conductivity and poor capacity retention impede the development of Mn-rich phosphate cathodes. Here, a light-weight F doping strategy is reported to modify high-voltage Na4MnCr(PO4)3 cathodes. The light-weight F dopant could decrease the energy gap to 0.22 eV from 1.52 eV and strengthen the adjacent chemical bonding around Mn as confirmed by density functional theory calculations, which ensure the boosted electronic conductivity and suppress Mn dissolution. The combination of in-situ and ex-situ techniques determine that the F dopant has no influence on the Na+ storage mechanisms. As a result, an outstanding rate performance up to 40 C and an improved cycling stability (1000 cycles at 20 C) are thus achieved (best reported performances until now). This work presents an unusual and widely available light-weight anion doping strategy for high-performance polyanionic cathodes.
Postoperative multidimensional risk factor analysis and nomogram construction in patients with non-small-cell lung cancer
Authors: Wu Lei , Yang Shanlan , Wang Nan , Zhang Pin , Chen Xintai , Dai Xiwang , Yu Chao , Jiang Han 
Published: July 2022

Abstract
BackgroundThe aim of this study was to investigate the multidimensional factors affecting overall survival (OS) and progression-free survival (PFS) of patients undergoing non-small-cell lung cancer (NSCLC) resection, and to construct nomograms for visualization based on the consideration of competing risk events.MethodsThis was a prospective, longitudinal cohort study conducted in the Second Affiliated Hospital of Nanchang University from to 2015-2021. We actively collected multidimensional information from 674 patients with NSCLC who underwent surgery in the first three years and were followed up for three years postoperatively. A competitive risk model was used to analyze the factors affecting the prognosis of patients with NSCLC, and a nomogram was developed for individualized evaluation. The effectiveness of the prediction models was measured using the concordance index (C-index), receiver operating characteristic (ROC) curve, and calibration curve.ResultsAge at surgery, number of lymph nodes (LNs) obtained during surgery, T stage, N stage, and prognostic nutritional index (PNI) can predict OS. Addition of adjuvant therapy to this basis can predict PFS. The C-index for the OS nomogram was 0.778 and the AUC was 0.788 (95% CI: 0.667–0.762). The C-index for the PFS nomogram was 0.751 and the AUC was 0.750 (95% CI: 0.681–0.820). The 2-year and 3-year calibration curves also showed good predictive performance.ConclusionsAge at surgery, number of lymph nodes, T stage, N stage, PNI, and adjuvant therapy are relevant in the prognostic evaluation of NSCLC patients undergoing surgery, and the constructed nomograms and competing risk models have good predictive value.
Prevalence of the anconeus epitrochlearis muscle in a Turkish population and its general characteristics on magnetic resonance imaging
Authors: Duran Semra , Gunaydin Elif , Cayhan Velihan 
Published: July 2022

Abstract
Introduction: The anconeus epitrochlearis (AE) muscle is an accessory muscle located between the medial cortex of the olecranon and the inferior surface of the medial epicondyle, posterior to the ulnar nerve. We aimed to determine the gender and side characteristics of the AE muscle in patients who underwent elbow magnetic resonance imaging (MRI) and report its prevalence in a Turkish population.Materials and Methods: A total of 209 patients (210 elbows) who underwent elbow MRI between January and December 2020 were retrospectively evaluated. Cases with the AE muscle were included in the study. The craniocaudal (CC) dimension was examined from the coronal section, and the transverse (TR) and anteroposterior (AP) dimensions from the axial sections. Statistical analyses were performed to determine the relationship between the presence of the AE muscle and age, gender, and side (right/left). Differences between sexes and sides in terms of muscle sizes were evaluated using statistical tests.Results: The AE muscle was detected in 17 patients (18 elbows) (8.1%). The mean age of the patients was 41.52 ± 14.63 years. There was no statistically significant difference between the male and female patients in terms of age. This accessory muscle was found in nine female (9.8%) and eight male (6.3%) patients in total. There was one (0.5%) patient with the bilateral AE muscle, who was female. The accessory muscle was located in the right elbow in 13 patients (10.71%), and left elbow in five (4.10%). It was more common in the right elbow in both genders. The muscle was larger in the male patients and the right elbow, but this was not statistically significant.Conclusion: The prevalence of the AE muscle was determined to be 8.1% in a Turkish population, and this muscle was more common among the women and in the right elbow.
Comparison of extra virgin olive oil (Olea Europea L.) and soybean oil (Glycine max L. Meer) subjected to adulteration
Authors: Ariza-Ortega José Alberto , Ariza-Torres Diana Gabriela , Trinidad Eva María Molina , Reyes Joel Díaz 
Published: July 2022

Abstract
The objective of this work was to compare extra virgin olive oil (EVOO) and soy oil (SO) subjected to adulteration. Two commercial brand oils were used. The adulteration of the EVOO was carried out by adding SO at 50/50%, 40/60%, 30/70%, 20/80% and 10/90% respectively. For each of the samples, relative density (δ), acidity index (AI) and refractive index (RI) were determined. The EVOO and SO presented a δ and a RI similar to that reported by the standards and researchers with 0.88 and 0.91 g cm− 3 and ≤ 1.467 and 1.470 ≥ respectively. Only SO had a degradation of FA (0.93% oleic acid). When was inducing the adulteration in all concentrations, the δ was modified (0.90 g cm− 3) and RI (1.469 until 1.473), and AI was constant (0.56%). The RI is the best option to be used in the detection of adulteration.
Two Dimensional Volterra Integrodifferential Equation of Heat Conduction and Diffusion Problems and Its Numerical Solutions
Authors: Al-Bugami A. M. 
Published: July 2022

Abstract
This paper deals with new equation in the form Two-dimensional Volterra integrodifferential equation (TDVIDE), which is related of the heat conduction and diffusion problems. Runge–Kutta method (RKM) and Bolck-by-Block method (BBM) have been applied to the problem. Numerical examples are presented, and their results are compared with the analytical solution to demonstrate the validity and applicability of the methods.
Regional neuroinflammation caused by peripheral infection is involved in fatigued symptoms: A [18F]DPA-714 PET study
Authors: Ochi Yuta , Hu Di , Li Danxi , Arakaki Wakiko , Mawatari Aya , Shigeta Mika , Wu Yuping , Hayashinaka Emi , Neyama Hiroyuki , Tahara Tsuyoshi , Wada Yasuhiro , Li Feng , Doi Hisashi , Watanabe Yasuyoshi , Cui Yilong 
Published: July 2022

Abstract
A series of symptoms, including fever, widespread pain, fatigue, and even ageusia, have frequently been reported in the context of various infections, such as COVID-19. Although the pathogenic mechanisms underlying an infection causing fever and pain have been well established, the mechanisms of fatigue induced by infection remain unclear. To elucidate whether and how the peripheral infection cause fatigue via regional neuroinflammation, we performed a brain-wide investigation of neuroinflammation in a peripheral pseudoinfection rat model using [18F]DPA-714 positron emission tomography (PET) imaging analysis, in which the polyriboinosinic: polyribocytidylic acid (poly I:C) was intraperitoneally injected. Consistent with previous reports, transient fever lasting for several hours and subsequent suppression of spontaneous activity lasting a few days were induced by poly I:C treatment. Significant increase in plasma interleukin (IL)-1β, IL-6 and tumour necrosis factor (TNF)-α were observed at 2 and 4 h following poly I:C treatment. PET imaging analysis revealed that the brain uptake of [18F]DPA-714 was significantly increased in several brain regions one day after poly I:C treatment, such as the dorsal raphe (DR), parvicellular part of red nucleus (RPC), A5 and A7 noradrenergic nucleus, compared with the control group. The accumulation of [18F]DPA-714 in the DR, RPC and A5 was positively correlated with the fatigue-like behavior, and that in the A7 tended to positively correlate with fever. These findings suggest that peripheral infection may trigger regional neuroinflammation, which may cause specific symptoms such as fatigue. A similar mechanism might be involved in COVID-19.
Anti-Virus Isoindolinone alkaloids with rare oxocyclopenta[f]isoindole frameworks isolated from the stems of flue cured tobacco
Authors: Hu Qiu-Fen , Ma Yue-Yu , Liu Hua-Yin , Dai Jia-Meng , Yang Feng-Xian , Zhang Jian-Duo , Wang Jin , Li Xue-Mei , Liu Xin , Li Jing , Li Yin-Ke , Wang Wei-Guang , Zhou Min , Yang Guang-Yu 
Published: July 2022

Abstract
Background
The large scale tobacco planting in Yunnan produces a large amount of waste, including stems, roots, low grade and defective tobacco leaves. Therefore, the multipurpose utilization of the by-product of tobacco is an interesting topic. In order to increase medicinal value of tobacco and the comprehensive utilization of its by-products, our group had investigated the chemical constituents of the stems of Y-202, a cultivar of tobacco with high resistance to tobacco mosaic virus (TMV), which originated from K326 by ethylmethane sulfonate (EMS) mutagenesis.
Results
As a result, four new isoindolinone-type small molecule alkaloids named nicoisoindoles A–D (1–4), along with four known isoindole derivatives (5–8) were isolated. Nicoisoindoles A–D represent a new subclass of isoindolinone alkaloids with rare cyclopenta[f]isoindole-1-one frameworks. Among them, nicoisoindole C (3) possesses an unusual N-2-(5-methoxy-6-methylpyridin-2-yl)ethyl moiety, while nicoisoindole D (4) has a novel a N-(3-methyl-6-oxo-1,6-dihydropyridin-2-yl)methyl substituent. Interestingly, compounds 1, 3, and 4 showed high anti-TMV activities with inhibition rates of 43.8, 58.8, and 67.8% at the concentration of 20 µM, and IC50 values of 23.6, 19.5 and 15.4 µM, respectively, even more potent than that of positive control. In order to study whether the oxocyclopenta[f]isoindole-1-ones have more broad antiviral activities, compounds 1–4 were also tested for their anti-rotavirus activities, and they exhibited potential anti-rotavirus activities with therapeutic index (TI) valves in the range of 13.0-18.5.
Conclusions
The successful isolation and structure identification of oxocyclopenta[f]isoindole-1-ones provide materials for the screening of anti-virus activities inhibitors, and also contribute to the development and utilization of the waste from tobacco cultivation.
Analyzing the genome-wide expression of m5C-related regulator genes and prognostic characteristics in pan-cancer
Authors: Zhang Jin , Wang Shuai , Li Guanghao , Duan Yuansheng , Wang Guowen , Zhang Yan 
Published: July 2022

Abstract
Background
It has been reported that 5-methylcytosine (m5C) is involved in DNA/RNA metabolic regulation and has significant implications for cancer diagnosis. M5C-related regulator genes have been reported involved in tumor progression, such as pancreatic adenocarcinomas, low-grade gliomas, and hepatocellular carcinoma, while there have been few studies on the genes in pan-cancers.
Methods
12 m5C-related regulator genes [Y-Box Binding Protein 1 (YBX1), Y-Box Binding Protein 12 (YBX2), NOP2/Sun RNA Methyltransferase 2 (NSUN2), NOP2/Sun RNA Methyltransferase 3 (NSUN3), NOP2/Sun RNA Methyltransferase 4 (NSUN4), NOP2/Sun RNA Methyltransferase 5 (NSUN5), NOP2/Sun RNA Methyltransferase 6 (NSUN6), NOP2/Sun RNA Methyltransferase 27 (NSUN7), DNA Methyltransferase 1 (DNMT1), DNA Methyltransferase 3 Alpha (DNMT3A), DNA Methyltransferase 3 Beta (DNMT3B), tRNA Aspartic Acid Methyltransferase 1 (TRDMT1)] were evaluated for their genomic alterations and clinical relevance across cancers. We performed single nucleotide variations (SNV), copy number variations (CNV), methylation variation, and miRNA-mRNA interactions analysis for patients of 33 cancer types from the Cancer Genome Atlas (TCGA). Based on the Cancer Therapeutics Response Portal (CTRP) and the Genomics of Drug Sensitivity in Cancer (GDSC) database, we assessed the sensitivity of drugs targeting the m5C-related regulator genes.
Results
Several m5C-related regulator genes have undergone significant genetic alterations, and their expression levels are significantly correlated with the activity of cancer hallmark-related pathways. Furthermore, m5C-related regulator genes may serve as used as a prognostic biomarker for cancers. We discovered a number of drugs that may target m5C-related regulator genes by drug sensitivity analysis.
Conclusion
This study revealed the genomic alterations and clinical characteristics of m5C-related regulator genes across 33 cancers, which may clarify the potential mechanism between m5C-related regulator genes and tumorigenesis and provide a novel approach for cancer treatments.
DC Electrical Stimulation effects on N2a and MC3T3 cell lines
Authors: Martín Daniel , Bocio Jesús , Scagliusi Santiago F , Pérez Pablo , Huertas Gloria , Yúfera Alberto , Giner Mercè , Daza Paula 
Published: July 2022

Abstract
Background: Electrical stimulation is a novel tool to promote the differentiation and proliferation of precursor cells. In this work we have studied the effects of direct current (DC) electrical stimulation on neuroblastoma (N2a) and osteoblast (MC3T3) cell lines as a model for nervous and bone tissue regeneration, respectively. We have developed the electronics and encapsulation of a proposed stimulation system and designed a setup and protocol to stimulate cell cultures. Methods: Cell cultures were subjected to several assays to assess the effects of electrical stimulation on them. N2a cells were analyzed using microscope images and an inmunofluorescence assay, differentiated cells were counted and neurites were measured. MC3T3 cells were subjected to an AlamarBlue assay for viability, ALP activity was measured, and a real time PCR was carried out.Results: Our results show that electrically stimulated cells had more tendency to differentiate in both cell lines when compared to non-stimulated cultures, paired with a promotion of neurite growth and polarization in N2a cells and an increase in proliferation in MC3T3 cell line.Conclusions: These results prove the effectiveness of electrical stimulation as a tool for tissue engineering and regenerative medicine, both for neural and bone injuries. Bone progenitor cells submitted to electrical stimulation have a higher tendency to differentiate and proliferate, filling the gaps present in injuries. On the other hand, neuronal progenitor cells differentiate, and their neurites can be polarized to follow the electric field applied.
Intranasal administration of NeuroEPO does not affect the structure of respiratory mucosa in Wistar rats.
Authors: Suárez Ketty , Fernández Gisselle , Cruz Yamila Rodríguez , Seguí Giselle Puldón 
Published: July 2022

Abstract
Introduction:
Diseases associated with the Central Nervous System (CNS) represent a group of conditions with important social and economic repercussions. New treatment strategies with NeuroEPO offer new opportunities to prevent the onset and progression of these disorders. NeuroEPO administered intranasally can reach the CNS through various mechanisms involving the olfactory and trigeminal nerves, respiratory and olfactory mucosa, nasal vasculature, cerebrospinal fluid, and the lymphatic system.
Objective
To determine the effect of intranasal administration of NeuroEPO on the histologic structure of the respiratory mucosa and its associated lymphatic tissue in Wistar rats.
Material and methods
an experimental, descriptive, longitudinal, prospective study was conducted, using the Wistar rat as a biological model. Ten healthy animals were randomly distributed in two groups of five animals each. One of the groups received intranasal NeuroEPO for 28 days at doses of 300 µg/kg. The other group was given a vehicle at a rate of 0.3µl/g. The histological characteristics of the respiratory mucosa were studied. The Mann-Whitney test was used to compare the means
Results
No alterations in the histological characteristics of the respiratory mucosa and the lymphatic tissue associated with the nasal mucosa were observed in Wistar rats after the administration of NeuroEPO.
Conclusion
Intranasal administration of NeuroEPO does not cause pathological changes in the histological structure of the respiratory mucosa or the lymphatic tissue associated with the nasal mucosa of Wistar rats in our experimental conditions.
Recovery of diaphragm function after neuromuscular block by means of diaphragm ultrasonography with comparison of neostigmine vs normal saline as reversal drugs
Authors: Chen Xuejiao , Yang Quanyong , Pan Qian , Zhang Zhenzhen , Yin Yiqing 
Published: July 2022

Abstract
Background
Residual neuromuscular blockade (RNMB) is a well-known risk factor after general anesthesia for critical respiratory events and higher postoperative morbidity. Acetylcholinesterase inhibitors cannot completely avoid RNMB. Neuromuscular monitoring of the adductor pollicis is the gold standard for detecting RNMB, but it cannot be carried out on conscious patients. Ultrasonography of diaphragm excursion may reveal residual effects of neuromuscular blocking agents in conscious patients.
Methods
This prospective, double-blind, single-center randomized controlled study enrolled 150 patients, who belong to ASA physical status I and II, aged 18–65 years, and be scheduled to undergo neuromuscular block with cis-atracurium for neurosurgery. The primary objective will be to compare the difference in DE between groups. Patients will be extubated when the train-of-four ratio is ≥ 0.9. Diaphragm ultrasonography will be used to evaluate the diaphragm excursion. The Diaphragm ultrasonography will be performed before anesthesia induction, at one minute after extubation, and before being discharged from the PACU. The secondary objective was to compare the incidence of postoperative pulmonary complications after operation.
Results
After extubation, the DE of patients was significantly larger in the NEO group than in the NS group (14.14 ± 1.93 vs. 12.96 ± 1.62 mm, P < 0.05). The incidence of RNMB was higher in the NS group, when compared to the NEO group, after extubation (73.33% vs. 53.33%, P = 0.011) and when leaving the PACU (33.33% vs. 20%, P = 0.044). The incidence of respiratory insufficiency after extubation was higher in the NEO group (14.67%), when compared to the NS group (4%) (P = 0.046).
Conclusion
Residual neuromuscular blockade is still a problem to be solved. Diaphragm ultrasound may become a bedside tool in the neuromuscular monitoring field to detect underestimated residual neuromuscular blockade after neuromuscular block.
Trial registration:
The trial was registered prior to patient enrollment at ClinicalTrials.gov (ChiCTR1900021095, Date of registration: January 28, 2019).