bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determining of water bioremediation dosage in recirculating water system for Cantik Grouper (Epinephelus fuscoguttatus × Epinephelus microdon) nurseries
Authors: Astari B , Ismi S , Mahardika K 
Published: July 2022

Abstract
The use of recirculating aquaculture system (RAS) in nurseries of cantik grouper (Epinephelus fuscoguttatus × Epinephelus microdon) has the potential to make the culture environment more stable and maximize production perfomance, but the work of RAS decreases with the accumulation of waste in the water used. Remediation of water with microorganisms (bioremediation) can improve the performance of RAS. This research aims to determine the best dose of commercial probiotics in water bioremediation to be used in nurseries of cantik grouper seeds. This research was conducted for 7 days using a completely randomized design with four treatments and three replications. Treatment doses of probiotics tried: 3 ml/m3, 6 ml/m3, 9 ml/m3 and 12 ml/m3. The container used was a concrete tank measuring 1 m × 2 m × 0.8 m filled with 1 m3 of seawater and 3.2±0.3 cm of cantik grouper seeds with a density of 500 fish/m3. The addition of probiotics up to 12 mL/m3 had no significant effect (P>0.05) on the survival rate of cantik grouper fish for 7 days of rearing, ranging from 99.1% to 99.3%. The best results of the bioremediation process were obtained with the addition of 6 mL/m3 probiotics with the lowest range of ammonia and nitrite about 0.0896–0.9303 mg/L and 0.0977–8.4880 mg/L. The addition of a probiotic dose of 6 mL/m3 can inhibit the growth of pathogenic bacteria such as Vibrio spp. in water at the beginning, middle, and end of maintenance reached 2, 48, and 140 CFU/mL.
Polysaccharide isolation of Brown seaweed: Sargassum sp and its photoprotection activity
Authors: Panjaitan M A P , Kasitowati R D , Putri N , Yamindago A , Asmara R , Aliviyanti D , Pratiwi D C 
Published: July 2022

AbstractSun radiation contains ultraviolet rays consisting of UV-A, UV-B, and UV-C. This ultraviolet light can cause adverse effects on the skin such as the appearance of sunburn (erythema), pigmentation, skin aging, and can also cause skin cancer (melanoma). To reduce the adverse effects on the skin, it is necessary to use sunscreen that protects the skin from exposure to ultraviolet rays. Brown seaweed is one of the natural ingredients that can be used as a natural ingredient for sunscreen. This is because genetically brown seaweed is more often exposed to sunlight so it can synthesize components that can absorb ultraviolet light.Sargassum sp.It is known to have polysaccharide compounds such as alginate and fucoidan which have potential as antioxidants and anti-inflammatory. The purpose of this study was to determine the photoprotective activity of the polysaccharide compounds found inSargassumsp.
Proximate Analysis of Fisheries Dried Products from Mamuju Traditional Market Post-Earthquake Disaster
Authors: Saputra Eka , Isroni Wahyu , Basarah Arief Rubiana , Andriyono Sapto 
Published: July 2022

AbstractThe earthquake in West Sulawesi that occurred in the last few months has disrupted several fishing facilities and activities. Public buildings and facilities were damaged, which caused deep trauma to the communities in Mamuju and Majene. This research was conducted during the recovery of post-earthquake conditions in Mamuju, West Sulawesi. Proximate analysis was carried out on three traditional processed products of the Mamuju community, which can be found in the traditional market of the Mamuju Seaport in the form of 2 dried anchovies and one dried shrimp product. The test results on the three products showed that the protein content was 43.22-44.03% and fat 2.92-3.14%, which indicated the quality of the processed product was quite good. Furthermore, the dried shrimp product contains protein, fat, 37.0% and 0.39%, respectively. This fishery product is quite potential for preparing nutritious food ingredients, but product packaging is still minimal and allows for a faster quality deterioration process. In addition, government support through related agencies in the form of assistance in improving the quality of processed products in Mamuju must be increased. The fishery resources in West Sulawesi is generally very potential to be developed as a source of income for regional development in the future.
The effect addional omega-3 in feed on the gonadal maturity level and fecundity of female silver rasbora, Rasbora argyrotaenia
Authors: Nabila H , Mubarak A S , Budi D S 
Published: July 2022

Abstract
Silver rasbora (Rasbora argyrotaenia) is a high-value commodity. The problem in cultivation of silver rasbora is long goanadal maturation period. The gonadal maturation period can be accelerated by manipulation of feed using omega-3. Omega-3 can affect reproductive performance, egg development, and fish embryogenesis. The purpose of this study was to determine effect of addition of omega-3 in feed on gonadal maturity level and fecundity of silver rasbora. The observed parameters are gonadal maturity level, gonado somatic index, gonado index and fecundity. This research applied a completely randomized design (CRD) method which consist of four treatments, namely control (0 mg/kg feed), P1 (2500 mg/kg feed), P2 (5000 mg/kg feed), and P3 (7500 mg/kg feed). The result of this study was indicate addition of omega-3 to feed increased gonadal maturity at GML IV stage and had a signicantly effect on the gonado somatic index, gonado index, and fecundity of female silver rasbora. The optimal dose of omega-3 for silver rasbora gonads is 7500 mg/kg of feed, which results in an increase in gonadal maturity to GML IV with a GSI of 18,89 ± 2,24%, a GI of 143,63 ± 6,66 and fecundity of 924,76 ± 9,21 eggs/parent.
The effect of bromelain enzyme on pineapple core on the SR, SGR, and FCR in carp (Cyprinus carpio) infested with Argulus japonicus
Authors: Rahmawati Y , Kismiyati  , Fahturohman A B 
Published: July 2022

Abstract
Carp (Cyprinus carpio) is a freshwater fish that has several advantages such us fast growth, easy maintenance, good nutritional value and economic value. The demand for carp products is quite high. Based on data from the Directorate General of Aquaculture (2018), there was an increase in production of 33,954 tons from 2010 to 2017. According to Faroq (2020), feeding mixed with pineapple core enzymes can increase the highest daily growth rate and feed efficiency. A more efficient feed is expected for fish to survive more when infected with the Argulus japonicus parasite. The aim of this study was to determine the effect of bromelain enzyme on pineapple core on SR, SGR, and FCR in carp (Cyprinus carpio) infested with Argulus japonicus. This study used a factorial completely randomized design with of 4x2 treatments with bromelain enzyme doses of 0% and 2.25% and the degree of argulus infestation, it is mild, moderate, and severe. The results showed that survival rate (SR), specific growth rate (SGR) and feed conversion (FCR) were not significantly different from use bromelain enzyme in carp infested with Argulus japonicus.
The effects of exogenous dietary enzyme on the growth of giant gourami (Osphronemus goramy)
Authors: Imaniy A N , Lamid M , Budi D S 
Published: July 2022

Abstract
The commercial complex enzymes containing protease, lipase, and amylase are potentially used for exogenous dietary enzymes on giant gourami (Osphronemus goramy) as growth booster indirectly. The aims of this study are to know the effect of the exogenous dietary enzymes on giant gourami growth and to obtain the optimal dose of this enzyme. The research was conducted on January-April 2019 in the aquaculture facility and laboratory of Universitas Airlangga, Banyuwangi Campus. A total of 200 giant gourami juveniles (5.5 ± 0.5 cm in total length) were obtained from the fish hatchery centre of Kabat, Banyuwangi, Indonesia. All fish was divided into 20 glass aquaria (40 x 30 x 30 cm3) and reared for 40 days with exogenous dietary enzyme treatments in the diets following: control (no enzyme), 2.5 ml/100 g feed, 5.0 ml/100 g feed, 7.5 ml/100 g feed, 10.0 ml/100 g feed (4 replication in each treatment). The parameters were observed in this study are daily growth rate (DGR), specific growth rate (SGR), and feed conversion ratio (FCR). The exogenous dietary enzyme significantly (P < 0.05) effect to all parameters, the best result was showed in 10.0 ml/100 g feed treatment as optimum applicate dose.
The effect of different stocking densities on ammonia (NH3) and nitrate (NO3) concentration on striped snakehead (Channa striata) culture in the bucket
Authors: Meidiana A , Prayogo  , Rahardja B S 
Published: July 2022

Abstract
Fish farming in the bucket is a fishery technology that is being developed using an aquaponic system. Striped snakehead fish (Channa striata) is one of the economically important types of fish but the cultivation of striped snakehead fish using an aquaponic system has never been carried out, so it is necessary to know the optimal stocking density of striped snakehead fish to minimize the risk of stress for fish. This study aims to determine the effect of different stocking densities on ammonia (NH3) and nitrate (NO3) concentration on striped snakehead (Channa striata) culture in the bucket. This research using 5 treatments and 4 replications, namely P0 1 fish/Liters; P1 2 fish/Liters; P2 3 fish/Liters; P3 4 fish/Liters; and P4 5 fish/Liters. The observed parameters are ammonia (NH3) and nitrate (NO3) concentration. The results of this study showed that the value of the ammonia concentration was between 0,089-0,609 mg/L and the nitrate concentration was between 0,931-1,539 mg/L. Based on the results of this study, it can be cocluded that treatment of different stocking density affect the concentration of ammonia (NH3) and nitrate (NO3) in the cultivation of Snakehead fish (Channa striata) in the bucket.
Nanoscale acoustic waves detection enhanced by edge plasmon mode resonance in nanoapertures
Authors: Wang Yanhong , Ji Baoqing , Wu Jingzhi , Piao Hang , Li Mengwei 
Published: July 2022

AbstractNanoapertures in metal films confine light to subwavelength dimensions generating enhanced electromagnetic fields. Acoustic resonances of nanostructures can be directly excited by dual frequency lasers due to intensity beating and induce the change of the refractive index in nanoaperture. Here we present that nanoapertures consisting of two intersecting holes support edge plasmons in the form of whispering-gallery modes which are highly sensitive to the refractive index of the surrounding environment. The refractive index variation caused by acoustic resonances in the nanostructure results in a significant change of transmission through the nanoaperture. Experimental results indicate that edge plasmons in the cavity of double nanohole help to minimize radiative losses via stronger confinement and increase acoustic detecting sensitivity. The edge plasmon modes in nanostructures may find applications in nanoparticle trapping, biosensors and light matter interactions in nanofluidics.
Variable Eating Patterns: A Potential Novel Risk Factor for Systemic Inflammation in Women
Authors: Makarem Nour , Zuraikat Faris M , Caceres Billy , Sears Dorothy D , St-Onge Marie-Pierre , Lai Yue , Aggarwal Brooke 
Published: July 2022

Abstract

Background
The timing and regularity of eating patterns could play a role in systemic inflammation, as circadian clocks responsible for daily rhythms of inflammatory signaling are entrained by food intake.

Purpose
To evaluate associations of intra-weekly and weekday-weekend differences in eating timing patterns with high-sensitivity C-reactive protein (hsCRP).

Methods
A community-based sample of 103 U.S. women from the American Heart Association Go Red for Women Strategically Focused Research Network completed a meal-timing questionnaire and provided a blood sample for measurement of hsCRP. Differences in weekday versus weekend eating start time, eating end time, and nightly fasting duration were calculated as eating jetlag metrics. Intra-weekly variability in eating timing patterns was defined by the standard deviation (SD) of these variables. Multivariable linear regression models were used to evaluate cross-sectional associations of eating timing variability metrics with hsCRP.

Results
Each additional 30-min difference in weekday–weekend eating end time was related to 13% higher hsCRP (p = .023). Similarly, every 30-min increase in eating end time SD, reflecting greater variability in timing of last eating occasion, was associated with 29% higher hsCRP. Per 1-hr weekday–weekend difference in nightly fasting duration, there was a 45% elevation in hsCRP (p = .003). Every 30-min increase in nightly fasting duration SD, representing greater variability in span of the daily fasting/eating periods, was associated with 46% higher hsCRP.

Conclusions
Variable eating timing patterns were associated with higher hsCRP. Intervention studies are needed to determine whether stabilizing the timing of eating occasions may represent a novel strategy to reduce chronic inflammation.
PSnoD: identifying potential snoRNA-disease associations based on bounded nuclear norm regularization
Authors: Sun Zijie , Huang Qinlai , Yang Yuhe , Li Shihao , Lv Hao , Zhang Yang , Lin Hao , Ning Lin 
Published: July 2022

Abstract
Many studies have proved that small nucleolar RNAs (snoRNAs) play critical roles in the development of various human complex diseases. Discovering the associations between snoRNAs and diseases is an important step toward understanding the pathogenesis and characteristics of diseases. However, uncovering associations via traditional experimental approaches is costly and time-consuming. This study proposed a bounded nuclear norm regularization-based method, called PSnoD, to predict snoRNA-disease associations. Benchmark experiments showed that compared with the state-of-the-art methods, PSnoD achieved a superior performance in the 5-fold stratified shuffle split. PSnoD produced a robust performance with an area under receiver-operating characteristic of 0.90 and an area under precision-recall of 0.55, highlighting the effectiveness of our proposed method. In addition, the computational efficiency of PSnoD was also demonstrated by comparison with other matrix completion techniques. More importantly, the case study further elucidated the ability of PSnoD to screen potential snoRNA-disease associations. The code of PSnoD has been uploaded to https://github.com/linDing-groups/PSnoD. Based on PSnoD, we established a web server that is freely accessed via http://psnod.lin-group.cn/.
Nogo-A reduces ceramide de novo biosynthesis to protect from heart failure
Authors: Sasset Linda , Manzo Onorina Laura , Zhang Yi , Marino Alice , Rubinelli Luisa , Riemma Maria Antonietta , Chalasani Madhavi Latha S , Dasoveanu Dragos C , Roviezzo Fiorentina , Jankauskas Stanislovas S , Santulli Gaetano , Bucci Maria Rosaria , Lu Theresa T , Di Lorenzo Annarita 
Published: July 2022

Abstract

Aims
Growing evidence correlate the accrual of the sphingolipid ceramide in plasma and cardiac tissue with heart failure (HF). Regulation of sphingolipid metabolism in the heart and the pathological impact of its derangement remain poorly understood. Recently, we discovered that Nogo-B, a membrane protein of endoplasmic reticulum, abundant in the vascular wall, down-regulates the sphingolipid de novo biosynthesis via serine palmitoyltransferase (SPT), first and rate liming enzyme, to impact vascular functions and blood pressure. Nogo-A, a splice isoform of Nogo, is transiently expressed in cardiomyocyte (CM) following pressure overload. Cardiac Nogo is up-regulated in dilated and ischaemic cardiomyopathies in animals and humans. However, its biological function in the heart remains unknown.

Methods and results
We discovered that Nogo-A is a negative regulator of SPT activity and refrains ceramide de novo biosynthesis in CM exposed to haemodynamic stress, hence limiting ceramide accrual. At 7 days following transverse aortic constriction (TAC), SPT activity was significantly up-regulated in CM lacking Nogo-A and correlated with ceramide accrual, particularly very long-chain ceramides, which are the most abundant in CM, resulting in the suppression of ‘beneficial’ autophagy. At 3 months post-TAC, mice lacking Nogo-A in CM showed worse pathological cardiac hypertrophy and dysfunction, with ca. 50% mortality rate.

Conclusion
Mechanistically, Nogo-A refrains ceramides from accrual, therefore preserves the ‘beneficial’ autophagy, mitochondrial function, and metabolic gene expression, limiting the progression to HF under sustained stress.
Predicting Early Literacy: Auditory and Visual Speech Decoding in Deaf and Hard-of-Hearing Children
Authors: Couvee Sascha , Wauters Loes , Verhoeven Ludo , Knoors Harry , Segers Eliane 
Published: July 2022

Abstract
The current study investigated the relative contributions of auditory speech decoding (i.e., auditory discrimination) and visual speech decoding (i.e., speechreading) on phonological awareness and letter knowledge in deaf and hard-of-hearing (DHH) kindergartners (Mage = 6;4, n = 27) and hearing kindergartners (Mage = 5;10, n = 42). Hearing children scored higher on auditory discrimination and phonological awareness, with the DHH children scoring at chance level for auditory discrimination, while no differences were found on speechreading and letter knowledge. For DHH children, speechreading correlated with phonological awareness and letter knowledge, for the hearing children, auditory discrimination correlated with phonological awareness. Two regression analyses showed that speechreading predicted phonological awareness and letter knowledge in DHH children only. Speechreading may thus be a compensatory factor in early literacy for DHH children, at least for those who are exposed to spoken language in monolingual or in bilingual or bimodal-bilingual contexts, and could be important to focus on during early literacy instruction.
Large RNP granules in Caenorhabditis elegans oocytes have distinct phases of RNA-binding proteins
Authors: Elaswad Mohamed T , Watkins Brooklynne M , Sharp Katherine G , Munderloh Chloe , Schisa Jennifer A 
Published: July 2022

Abstract
The germ line provides an excellent in vivo system to study the regulation and function of RNP granules. Germ granules are conserved germ line-specific RNP granules that are positioned in the Caenorhabditis elegans adult gonad to function in RNA maintenance, regulation, and surveillance. In Caenorhabditis elegans, when oogenesis undergoes extended meiotic arrest, germ granule proteins and other RNA-binding proteins assemble into much larger RNP granules whose hypothesized function is to regulate RNA metabolism and maintain oocyte quality. To gain insight into the function of oocyte RNP granules, in this report, we characterize distinct phases for four protein components of RNP granules in arrested oocytes. We find that the RNA-binding protein PGL-1 is dynamic and has liquid-like properties, while the intrinsically disordered protein MEG-3 has gel-like properties, similar to the properties of the two proteins in small germ granules of embryos. We find that MEX-3 exhibits several gel-like properties but is more dynamic than MEG-3, while CGH-1 is dynamic but does not consistently exhibit liquid-like characteristics and may be an intermediate phase within RNP granules. These distinct phases of RNA-binding proteins correspond to, and may underlie, differential responses to stress. Interestingly, in oocyte RNP granules, MEG-3 is not required for the condensation of PGL-1 or other RNA-binding proteins, which differs from the role of MEG-3 in small, embryonic germ granules. Lastly, we show that the PUF-5 translational repressor appears to promote MEX-3 and MEG-3 condensation into large RNP granules; however, this role may be associated with regulation of oogenesis.
Etrolizumab-s Does Not Induce Residual Trafficking of Regulatory T Cells
Authors: Schweda Anna , Becker Emily , Wiendl Maximilian , Atreya Raja , Atreya Imke , Müller Tanja M , Neurath Markus F , Zundler Sebastian 
Published: July 2022

Abstract

Background
Blocking immune cell gut homing via α4β7 integrin with the monoclonal antibody vedolizumab is an established therapeutic strategy in inflammatory bowel disease. However, despite promising preclinical and phase 2 clinical data, the anti-β7 antibody etrolizumab yielded disappointing results in a large phase 3 trial program in UC. Mechanistic explanations are still lacking. We have recently shown that vedolizumab is associated with residual homing of regulatory T (Treg) cells in a certain exposure range and aimed to investigate whether a similar mechanism applies for etrolizumab.

Methods
We used flow cytometry, competitive dynamic adhesion, and transmigration assays to assess binding of the etrolizumab surrogate (etrolizumab-s) antibody FIB504 to Treg and effector T cells (Teff) and to explore the impact on cell trafficking.

Results
We observed only minimal differences in the binding of etrolizumab-s to Treg and Teff cells. Dynamic adhesion and transmigration of Treg and Teff cells was not substantially differentially affected at relevant concentrations. The β1+ and PI16+ Treg cells were only resistant to etrolizumab-s at low concentrations.

Conclusions
Etrolizumab does not seem to induce notable residual trafficking of Treg cells. Thus, the Teff overweight in the inflamed gut might persist despite reduced overall T cell recruitment. This might be one piece of the puzzle to explain recent clinical results in phase 3.
A Comparative Approach to Understanding Floral Adaptation to Climate and Pollinators During Diversification in European and Mediterranean Silene
Authors: Miladin Jenna R , Steven Janet C , Collar David C 
Published: July 2022

Abstract
Pollinator selection on floral traits is a well-studied phenomenon, but less is known about the influence of climate on this species interaction. Floral trait evolution could be a result of both adaptation to climate and pollinator-mediated selection. In addition, climate may also determine pollinator communities, leading to an indirect influence of climate on floral traits. In this study, we present evidence of both direct and indirect effects of climate on plant morphology through a phylogenetic comparative analysis of the relationships between climate, pollinators, and morphology in 89 European and Mediterranean Silene species. Climate directly influences vegetative morphology, where both leaf size and internode length were found to be smaller in habitats that are warmer in the driest quarter of the year and that have more precipitation in the coldest quarter of the year. Similarly, flower size was directly influenced by climate, where smaller calyxes were also associated with habitats that are warmer in the driest quarter of the year. These results suggest that reduced leaf and flower size promote water conservation in species that occupy arid climates. Floral traits also evolved in response to pollinators, with elongated calyxes associated with nocturnal pollination, though we also found evidence that climate influences pollinator distribution. Nocturnal pollinators of Silene are found in habitats that have more temperature evenness across seasons than diurnal pollinators. Correspondingly, nocturnally pollinated Silene are more likely to occur in habitats that have lower daily temperature fluctuation and more temperature evenness across seasons. Altogether these results show that climate can directly influence vegetative and floral morphology, but it can also affect pollinator distribution, which in turn drives floral adaptation. Our study therefore suggests that climate mediates the influence of species interactions on trait evolution by imposing direct selective demands on floral phenotypes and by determining the pollinator community that imposes its own selective demands on flowers.