bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zyxin protects from hypertension-induced cardiac dysfunction
Authors: Al-Hasani Jaafar , Sens-Albert Carla , Ghosh Subhajit , Trogisch Felix A. , Nahar Taslima , Friede Prisca A. P. , Reil Jan-Christian , Hecker Markus 
Published: January 2022

AbstractArterial hypertension causes left ventricular hypertrophy leading to dilated cardiomyopathy. Following compensatory cardiomyocyte hypertrophy, cardiac dysfunction develops due to loss of cardiomyocytes preceded or paralleled by cardiac fibrosis. Zyxin acts as a mechanotransducer in vascular cells that may promote cardiomyocyte survival. Here, we analyzed cardiac function during experimental hypertension in zyxin knockout (KO) mice. In zyxin KO mice, made hypertensive by way of deoxycorticosterone acetate (DOCA)-salt treatment telemetry recording showed an attenuated rise in systolic blood pressure. Echocardiography indicated a systolic dysfunction, and isolated working heart measurements showed a decrease in systolic elastance. Hearts from hypertensive zyxin KO mice revealed increased apoptosis, fibrosis and an upregulation of active focal adhesion kinase as well as of integrins α5 and β1. Both interstitial and perivascular fibrosis were even more pronounced in zyxin KO mice exposed to angiotensin II instead of DOCA-salt. Stretched microvascular endothelial cells may release collagen 1α2 and TGF-β, which is characteristic for the transition to an intermediate mesenchymal phenotype, and thus spur the transformation of cardiac fibroblasts to myofibroblasts resulting in excessive scar tissue formation in the heart of hypertensive zyxin KO mice. While zyxin KO mice per se do not reveal a cardiac phenotype, this is unmasked upon induction of hypertension and owing to enhanced cardiomyocyte apoptosis and excessive fibrosis causes cardiac dysfunction. Zyxin may thus be important for the maintenance of cardiac function in spite of hypertension.
JAM-A interacts with α3β1 integrin and tetraspanins CD151 and CD9 to regulate collective cell migration of polarized epithelial cells
Authors: Thölmann Sonja , Seebach Jochen , Otani Tetsuhisa , Florin Luise , Schnittler Hans , Gerke Volker , Furuse Mikio , Ebnet Klaus 
Published: January 2022

AbstractJunctional adhesion molecule (JAM)-A is a cell adhesion receptor localized at epithelial cell–cell contacts with enrichment at the tight junctions. Its role during cell–cell contact formation and epithelial barrier formation has intensively been studied. In contrast, its role during collective cell migration is largely unexplored. Here, we show that JAM-A regulates collective cell migration of polarized epithelial cells. Depletion of JAM-A in MDCK cells enhances the motility of singly migrating cells but reduces cell motility of cells embedded in a collective by impairing the dynamics of cryptic lamellipodia formation. This activity of JAM-A is observed in cells grown on laminin and collagen-I but not on fibronectin or vitronectin. Accordingly, we find that JAM-A exists in a complex with the laminin- and collagen-I-binding α3β1 integrin. We also find that JAM-A interacts with tetraspanins CD151 and CD9, which both interact with α3β1 integrin and regulate α3β1 integrin activity in different contexts. Mapping experiments indicate that JAM-A associates with α3β1 integrin and tetraspanins CD151 and CD9 through its extracellular domain. Similar to depletion of JAM-A, depletion of either α3β1 integrin or tetraspanins CD151 and CD9 in MDCK cells slows down collective cell migration. Our findings suggest that JAM-A exists with α3β1 integrin and tetraspanins CD151 and CD9 in a functional complex to regulate collective cell migration of polarized epithelial cells.
The value of metabolic parameters and textural analysis in predicting prognosis in locally advanced cervical cancer treated with chemoradiotherapy
Authors: Pedraza Sara , Seiffert Alexander P. , Sarandeses Pilar , Muñoz-Lopez Beatriz , Gómez Enrique J. , Sánchez-González Patricia , Pérez-Regadera José F. 
Published: January 2022

Abstract
Objective
The aim of the study was to assess the impact of clinical and metabolic parameters derived from 18F-FDG PET/CT (positron emission tomography–computed tomography) in patients with locally advanced cervical cancer (LACC) on prognosis.

Methods
Patients with LACC of stage IB2-IVA treated by primary radiochemotherapy followed by brachytherapy were enrolled in this retrospective study. Indexes derived from standardized uptake value (SUV), metabolic tumor volume (MTV), total lesion glycolysis (TLG), and textural features of the primary tumor were measured for each patient. Overall survival (OS) and recurrence-free survival (RFS) rates were calculated according to Kaplan–Meier and survival curves were compared using the log-rank test. Uni- and multivariate analyses were performed using the Cox regression model.

Results
A total of 116 patients were included. Median follow-up was 58 months (range: 1–129). A total of 36 (31%) patients died. Five-year OS and RFS rates were 69 and 60%, respectively. Univariate analyses indicated that FIGO stage, the presence of hydronephrosis, high CYFRA 21.1 levels, and textural features had a significant impact on OS and RFS. MTV as well as SCC-Ag concentration were also significantly associated with OS. On multivariate analysis, the presence of hydronephrosis, CYFRA 21.1, and sphericity were independent prognostics factors for OS and RFS. Also, SCC-Ag level, MTV, and GLZLM (gray-level zone length matrix) ZLNU (zone length non-uniformity) were significantly associated with OS.

Conclusion
Classical prognostic factors and tumor heterogeneity on pretreatment PET/CT were significantly associated with prognosis in patients with LACC.
Positionspapier der Initiative Chronische Wunde (ICW) e. V. zur Nomenklatur des Débridements chronischer Wunden
Authors: Dissemond Joachim , Bültemann Anke , Gerber Veronika , Motzkus Martin , Münter Karl Christian , Erfurt-Berge Cornelia 
Published: January 2022

ZusammenfassungDie heute in der Wundbehandlung verwendete Nomenklatur ist interdisziplinär und interprofessionell sehr unterschiedlich. Daher ist es ein Anliegen der Fachgesellschaft Initiative Chronische Wunde (ICW) e. V. bislang unklare Begriffe eindeutig und nachvollziehbar zu beschreiben. Von den Experten der ICW wurde daher in einem Konsensusverfahren als Débridement chronischer Wunden die Entfernung von anhaftendem, abgestorbenem Gewebe, Krusten oder Fremdkörpern aus Wunden bezeichnet. Hierfür gibt es verschiedene Therapieoptionen, die als autolytisches, biochirurgisches, mechanisches, osmotisches, proteolytisches/enzymatisches und technisches Débridement unterschieden werden können. Bei dem chirurgischen Débridement wird zudem zwischen meist ambulant durchführbaren scharfen Débridements wie beispielsweise kleineren chirurgischen Eingriffen und chirurgischen Débridements mit adäquater Anästhesie in einem Operationssaal differenziert. Als Wundspülung wird von der ICW die Entfernung von nicht haftenden Bestandteilen auf Wunden mit sterilen Lösungen bezeichnet.Débridement und/oder Wundspülung sind oft der erste Schritt einer phasengerechten modernen Wundbehandlung. Mehrere Methoden eignen sich für die Anwendung einer kombinierten oder sukzessiven Therapie. Bei der Entscheidung, welche therapeutische Option hierbei zum Einsatz kommt, sollte eine Vielzahl individuell unterschiedlicher Faktoren in Abhängigkeit von den zu behandelnden Patienten, aber auch von den Therapeuten berücksichtigt werden. Die letztendliche individuelle Entscheidung für eine Methode sollte jeweils gemeinsam mit den Patienten getroffen und anschließend adäquat dokumentiert werden.
Pott’s puffy tumor: a need for interdisciplinary diagnosis and treatment
Authors: Kühn Jan Philipp , Linsler Stefan , Nourkami-Tutdibi Nasenien , Meyer Sascha , Becker Sören L. , Yilmaz Umut , Schick Bernhard , Bozzato Alessandro , Kulas Philipp 
Published: January 2022

AbstractPott’s puffy tumor (PPT) is an infection of the frontal sinus with subperiosteal and intracranial abscess formation and one of the rare entities in pediatrics. We present a series of four cases of PPT that occurred in two children (6 and 9 years) and in two young adults (17 and 19 years). All patients were treated by an interdisciplinary team of pediatric, neurosurgical, ENT, radiological, and neuroradiological specialists. Antibiotic treatment was combined with single endoscopic surgery in one case and combined endoscopic sinus surgery with an open transcranial approach to drain intracranial abscess formation in three cases. It is important to be aware that PPT occurs in children with the finding of intracranial abscess formation. Therefore, a close interdisciplinary cooperation for successful treatment is needed in this rare disease.
Machine learning for geochemical exploration: classifying metallogenic fertility in arc magmas and insights into porphyry copper deposit formation
Authors: Nathwani Chetan L. , Wilkinson Jamie J. , Fry George , Armstrong Robin N. , Smith Daniel J. , Ihlenfeld Christian 
Published: January 2022

AbstractA current mineral exploration focus is the development of tools to identify magmatic districts predisposed to host porphyry copper deposits. In this paper, we train and test four, common, supervised machine learning algorithms: logistic regression, support vector machines, artificial neural networks (ANN) and Random Forest to classify metallogenic ‘fertility’ in arc magmas based on whole-rock geochemistry. We outline pre-processing steps that can be used to mitigate against the undesirable characteristics of geochemical data (high multicollinearity, sparsity, missing values, class imbalance and compositional data effects) and therefore produce more meaningful results. We evaluate the classification accuracy of each supervised machine learning technique using a tenfold cross-validation technique and by testing the models on deposits unseen during the training process. This yields 81–83% accuracy for all classifiers, and receiver operating characteristic (ROC) curves have mean area under curve (AUC) scores of 87–89% indicating the probability of ranking a ‘fertile’ rock higher than an ‘unfertile’ rock. By contrast, bivariate classification schemes show much lower performance, demonstrating the value of classifying geochemical data in high dimension space. Principal component analysis suggests that porphyry-fertile magmas fractionate deep in the arc crust, and that calc-alkaline magmas associated with Cu-rich porphyries evolve deeper in the crust than more alkaline magmas linked with Au-rich porphyries. Feature analysis of the machine learning classifiers suggests that the most important parameters associated with fertile magmas are low Mn, high Al, high Sr, high K and listric REE patterns. These signatures further highlight the association of porphyry Cu deposits with hydrous arc magmas that undergo amphibole fractionation in the deep arc crust.
Chronic mild stress paradigm as a rat model of depression: facts, artifacts, and future perspectives
Authors: Strekalova Tatyana , Liu Yanzhi , Kiselev Daniel , Khairuddin Sharafuddin , Chiu Jennifer Lok Yu , Lam Justin , Chan Ying-Shing , Pavlov Dmitrii , Proshin Andrey , Lesch Klaus-Peter , Anthony Daniel C. , Lim Lee Wei 
Published: January 2022

AbstractRationaleThe chronic mild stress (CMS) paradigm was first described almost 40 years ago and has become a widely used model in the search for antidepressant drugs for major depression disorder (MDD). It has resulted in the publication of almost 1700 studies in rats alone. Under the original CMS procedure, the expression of an anhedonic response, a key symptom of depression, was seen as an essential feature of both the model and a depressive state. The prolonged exposure of rodents to unpredictable/uncontrollable mild stressors leads to a reduction in the intake of palatable liquids, behavioral despair, locomotor inhibition, anxiety-like changes, and vegetative (somatic) abnormalities. Many of the CMS studies do not report these patterns of behaviors, and they often fail to include consistent molecular, neuroanatomical, and physiological phenotypes of CMS-exposed animals.ObjectivesTo critically review the CMS studies in rats so that conceptual and methodological flaws can be avoided in future studies.ResultsAnalysis of the literature supports the validity of the CMS model and its impact on the field. However, further improvements could be achieved by (i) the stratification of animals into ‘resilient’ and ‘susceptible’ cohorts within the CMS animals, (ii) the use of more refined protocols in the sucrose test to mitigate physiological and physical artifacts, and (iii) the systematic evaluation of the non-specific effects of CMS and implementation of appropriate adjustments within the behavioral tests.ConclusionsWe propose methodological revisions and the use of more advanced behavioral tests to refine the rat CMS paradigm, which offers a valuable tool for developing new antidepressant medications.
Fractional mean curvature flow of Lipschitz graphs
Authors: Cesaroni Annalisa , Novaga Matteo 
Published: January 2022

AbstractWe consider the fractional mean curvature flow of entire Lipschitz graphs. We provide regularity results, and we study the long time asymptotics of the flow. In particular we show that in a suitable rescaled framework, if the initial graph is a sublinear perturbation of a cone, the evolution asymptotically approaches an expanding self-similar solution. We also prove stability of hyperplanes and of convex cones in the unrescaled setting.
Diagnostic accuracy of [18F]PSMA-1007 PET/CT in biochemical recurrence of prostate cancer
Authors: Mingels Clemens , Bohn Karl Peter , Rominger Axel , Afshar-Oromieh Ali , Alberts Ian 
Published: January 2022

Abstract
Aim
Despite increasing use for the detection of biochemically recurrent prostate cancer (rPC), the diagnostic accuracy of positron emission tomography/computed tomography (PET/CT) with [18F]PSMA-1007 remains only partially investigated. The aim of this study was to determine the sensitivity (SE), specificity (SP), positive predictive value (PPV), and negative predictive value (NPV) for PC-local recurrence and metastases on a per region basis.

Materials and methods
One hundred seventy-seven consecutive patients undergoing [18F]PSMA-1007 PET/CT for rPC were retrospectively analysed. Six body regions were defined: prostate fossa, pelvic lymph nodes (LN), retroperitoneal LN, supradiaphragmatic LN, bones, and soft tissue. A region was counted positive if at least one PSMA-positive lesion suspicious for PC was observed. Confirmation of a true-positive PSMA-avid lesion was defined as positive by histopathology, fall in serum prostate-specific antigen (PSA) (> 50%) after targeted therapy or confirmatory further CT, MRI, PET/CT, or bone scan imaging. Regions where additional imaging was able to confirm the absence of suspicious PC lesions or regions outside exclusively targeted RT with serum PSA decline (> 50%) were counted as true-negative regions. SE, SP, PPV, and NPV were calculated for all six regions.

Results
The overall PET-positivity rate was 91%. Conclusive follow-up for affirmation or refutation of a PSMA-positive lesion was available for 81/152 patients on a per region basis. In this subgroup, overall sensitivity, specificity, PPV, and NPV were 95% (CI: 0.90–0.98), 89% (CI: 0.83–0.93), 86% (0.80–0.90), and 96% (CI: 0.92–0.98), respectively. On a per region basis, PPV was 97% (CI: 0.83–0.99) for local recurrence, 93% (CI: 0.78–0.98) for pelvic LN, 87% (CI: 0.62–0.96) for retroperitoneal LN, 82% (CI: 0.52–0.95) for supradiaphragmatic LN, and 79% (0.65–0.89) for bone lesions. The number of solid organ metastases (n = 6) was too small for an accurate statistical analysis.

Conclusion
The known high PET-positivity rate of [18F]PSMA-1007 PET/CT in rPC was confirmed, with corresponding high (> 90%) sensitivity and NPV on a per region basis. However, overall PPV was limited (86%), particularly for bone lesions (79%), which are a potential diagnostic weaknesses when using this tracer.
Variation in the behaviour of an obligate corallivore is influenced by resource availability
Authors: Gunn Rachel L. , Hartley Ian R. , Algar Adam C. , Nadiarti N. , Keith Sally A. 
Published: January 2022

Abstract
Marine environments are subject to increasing disturbance events, and coral reef ecosystems are particularly vulnerable. During periods of environmental change, organisms respond initially through rapid behavioural modifications. Whilst mean population level modifications to behaviour are well documented, how these shifts vary between individuals, and the relative trade-offs that are induced, are unknown. We test whether the frequency and time invested in different behaviours varies both between and within individuals with varying resource availability. To do this, we quantify differences in four key behavioural categories (aggression, exploration, feeding and sociability) at two sites of different resource availability, using an obligate corallivore butterflyfish species (Chaetodon lunulatus). Individuals on a low resource site held larger territories, investing more time in exploration, which was traded off with less time invested on aggression, feeding and sociability. Repeatability measures indicated that behavioural differences between sites could plausibly be driven by both plasticity of behaviour within individuals and habitat patchiness within feeding territories. By combining population-level means, co-correlation of different behaviours and individual-level analyses, we reveal potential mechanisms behind behavioural variation in C. lunulatus due to differences in resource availability.

Significance statement
Using observational methods, we identify differences in the behaviour of an obligate corallivorous butterflyfish (Chaetodon lunulatus) between a high and a low resource site. We use a combination of density surveys, territory mapping and behavioural observation methods in a comparative analysis to relate behaviour directly to the environment in which it occurs. Bringing together insights from game theory and optimal foraging, we also use our results to highlight how understanding the correlations of different behaviours can inform our understanding of the extent to which behaviours are plastic or fixed. Furthermore, by considering how multiple behaviours are correlated, we move away from exploring individual behaviours in isolation and provide an in-depth insight into how differences in behaviour both between individuals and at the population level can affect responses to declining resource availability.
Effects of Silicone Breast Implants on Human Cell Types In Vitro: A Closer Look on Host and Implant
Authors: Colaris Maartje J. L. , Ruhl Tim , Beier Justus P. 
Published: January 2022

Abstract
Background
Silicone (gel) breast implants (SBI) are used world-wide for breast augmentation, and reconstruction or to correct breast deformities. They consist of two compounds: an elastomer silicone shell (envelope) and a silicone gel filler (core). Breast Implant Illness (BII) is a term used for women with SBI, who suffer from various of symptoms including myalgia, arthralgia, fatigue, fever, dry eyes and/or dry mouth (sicca), as well as cognitive disturbances, which are rated by these woman as response to SBI. The pathogenesis of these adverse effects as well as the histocompatibility and the SBI-cell interaction of silicone and its surrounding tissue (implant-host tissue interface) is a subject of current research. The main purpose of this review is to provide an overview of the current knowledge regarding the effects of silicone (gel and elastomer surfaces) of a SBI on different human cell types from experimental - in vitro - models.

Methods
A comprehensive research was conducted by two independent reviewers in March and July of 2020 in the PubMed, MEDLINE, and Cochrane databases.

Results
A number of 1328 articles on this topic were initially identified, of which 62 could be finally included an analysed in this review.

Conclusion
SBI may lead to a physiologic pro-inflammatory and foreign body host response with fibrous encapsulation accompanied by a disturbed Th17/Treg balance and IL-17 production. No causal relationship is known for systemic symptoms and/or autoimmune outcomes in the context of BII.

Level of Evidence III
This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
Can heat shock protein 70 (HSP70) serve as biomarkers in Antarctica for future ocean acidification, warming and salinity stress?
Authors: Yusof Nur Athirah , Masnoddin Makdi , Charles Jennifer , Thien Ying Qing , Nasib Farhan Nazaie , Wong Clemente Michael Vui Ling , Abdul Murad Abdul Munir , Mahadi Nor Muhammad , Bharudin Izwan 
Published: January 2022

AbstractThe Antarctic Peninsula is one of the fastest-warming places on Earth. Elevated sea water temperatures cause glacier and sea ice melting. When icebergs melt into the ocean, it “freshens” the saltwater around them, reducing its salinity. The oceans absorb excess anthropogenic carbon dioxide (CO2) causing decline in ocean pH, a process known as ocean acidification. Many marine organisms are specifically affected by ocean warming, freshening and acidification. Due to the sensitivity of Antarctica to global warming, using biomarkers is the best way for scientists to predict more accurately future climate change and provide useful information or ecological risk assessments. The 70-kilodalton (kDa) heat shock protein (HSP70) chaperones have been used as biomarkers of stress in temperate and tropical environments. The induction of the HSP70 genes (Hsp70) that alter intracellular proteins in living organisms is a signal triggered by environmental temperature changes. Induction of Hsp70 has been observed both in eukaryotes and in prokaryotes as response to environmental stressors including increased and decreased temperature, salinity, pH and the combined effects of changes in temperature, acidification and salinity stress. Generally, HSP70s play critical roles in numerous complex processes of metabolism; their synthesis can usually be increased or decreased during stressful conditions. However, there is a question as to whether HSP70s may serve as excellent biomarkers in the Antarctic considering the long residence time of Antarctic organisms in a cold polar environment which appears to have greatly modified the response of heat responding transcriptional systems. This review provides insight into the vital roles of HSP70 that make them ideal candidates as biomarkers for identifying resistance and resilience in response to abiotic stressors associated with climate change, which are the effects of ocean warming, freshening and acidification in Antarctic organisms.