bluuebunny/crossref_metadata_2025_split huggingface dataset:
Creeping yeast: a simple, cheap and robust protocol for the identification of mating type in Saccharomyces cerevisiae
Authors: Arras Samantha D M , Hibbard Taylor R , Mitsugi-McHattie Lucy , Woods Matthew A , Johnson Charlotte E , Munkacsi Andrew , Hermann-Le Denmat Sylvie , Ganley Austen R D 
Published: March 2022

Abstract
Saccharomyces cerevisiae is an exceptional genetic system, with genetic crosses facilitated by its ability to be maintained in haploid and diploid forms. Such crosses are straightforward if the mating type/ploidy of the strains is known. Several techniques can determine mating type (or ploidy), but all have limitations. Here, we validate a simple, cheap and robust method to identify S. cerevisiae mating types. When cells of opposite mating type are mixed in liquid media, they ‘creep’ up the culture vessel sides, a phenotype that can be easily detected visually. In contrast, mixtures of the same mating type or with a diploid simply settle out. The phenotype is observable for several days under a range of routine growth conditions and with different media/strains. Microscopy suggests that cell aggregation during mating is responsible for the phenotype. Yeast knockout collection analysis identified 107 genes required for the creeping phenotype, with these being enriched for mating-specific genes. Surprisingly, the RIM101 signaling pathway was strongly represented. We propose that RIM101 signaling regulates aggregation as part of a wider, previously unrecognized role in mating. The simplicity and robustness of this method make it ideal for routine verification of S. cerevisiae mating type, with future studies required to verify its molecular basis.
Fraud detection in motor insurance: privacy and data protection concerns under EU Law
Authors: Amankwah Jeffrey , Van Schoubroeck Caroline 
Published: March 2022

Abstract
This article focuses on the privacy and data protection concerns under European Union (EU) law with respect to the detection of insurance fraud in motor insurance.
Insurance fraud—in the context of this article—encompasses all claims where an insured intends to deceive an insurer, aiming to direct payment towards a person who is not the intended recipient, or to procure payment to which the insured is not entitled.
More and more frequently, insurers rely on advanced technologies (such as network analysis) to detect fraudulent claims. Evidently, these techniques need to operate within the boundaries of the law governing the protection of personal data and the right to privacy.
The EU General Data Protection Regulation imposes certain restrictions on the processing of personal data. This article analyses the legal restrictions Articles 6 and 22 General Data Protection Regulation impose on insurers with respect to the processing of personal data for the purpose of fraud detection.
Moreover, insurers work together to identify known fraudsters by sharing access to a database that contains registered cases of fraud (blacklists). This article analyses the legality of such blacklists in light of the EU law on data protection.
Stories about “Us” and “Them”: An Experimental Inquiry into the Relative Appeal of Populist Narratives
Authors: Yanchenko Kostiantyn 
Published: March 2022

AbstractDue to continued mediatization of politics, storytelling plays an increasingly important role in political communication. Against this background, it is often argued that populist narratives are essentially more appealing than non-populist political narratives, yet empirical evidence to support this assumption was missing. A multi-message experiment was conducted among U.S. citizens (N = 206) to compare how populist and non-populist political stories affect narrative transportation—a psychological state strongly associated with narrative enjoyment. The results show that populist framing significantly increases the ability of political narratives to cause transportation, controlling for the political partisanship and populist predispositions of the participants. This effect is mediated by the identification with story characters and feelings of anger and anxiety. The positive impact of populist framing extends to the story-consistent attitudes of the participants, suggesting a parallel–serial mediation model. The study enhances our understanding of psychological responses to populist storytelling.
Determination of GL(3) Cusp Forms by Central Values of Quadratic Twisted L-Functions
Authors: Hua Shenghao , Huang Bingrong 
Published: March 2022

Abstract
Let $\phi $ and $\phi ^{\prime}$ be two $\operatorname {GL}(3)$ Hecke–Maass cusp forms. In this paper, we prove that $\phi =\phi ^{\prime} \textrm {or }\widetilde {\phi ^{\prime}}$ if there exists a nonzero constant $\kappa $ such that $L(\frac {1}{2},\phi \otimes \chi _{8d})=\kappa L(\frac {1}{2},\phi ^{\prime}\otimes \chi _{8d})$ for all positive odd square-free positive $d$. Here, $\widetilde {\phi ^{\prime}}$ is dual form of $\phi ^{\prime}$ and $\chi _{8d}$ is the quadratic character $(\frac {8d}{\cdot })$. To prove this, we obtain asymptotic formulas for twisted 1st moment of central values of quadratic twisted $L$-functions on $\operatorname {GL}(3)$, which will have many other applications.
Dynamic evolution of imipenem/relebactam resistance in a KPC-producing Klebsiella pneumoniae from a single patient during ceftazidime/avibactam-based treatments
Authors: Gaibani Paolo , Bovo Federica , Bussini Linda , Lazzarotto Tiziana , Amadesi Stefano , Bartoletti Michele , Viale Pierlugi , Ambretti Simone 
Published: March 2022

Abstract

Objectives
The novel carbapenem/β-lactamase inhibitor combination imipenem/cilastatin/relebactam has been developed for the treatment of infections due to carbapenemase-producing Enterobacteriaceae (CPE). Herein, we describe the in vivo evolution of imipenem/cilastatin/relebactam resistance in longitudinal intra-patient Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPC-Kp) strains isolated from a patient following ceftazidime/avibactam-based treatments.

Methods
WGS analysis was performed on KPC-Kp strains isolated at different times and during antimicrobial treatments from the same patient. Genome assemblies were performed using a hybrid approach using Illumina iSeq 100 and Minion Oxford Nanopore platforms. Subpopulation analysis and allele frequency determination was performed by mapping Illumina reads to blaKPC.

Results
During antimicrobial treatment, resistance to ceftazidime/avibactam was observed following 16 days of antimicrobial therapy. WGS results showed that all KPC-Kp exhibited a low SNP rate of divergence, belonged to ST512 and shared similar antimicrobial resistance and porin gene patterns. Genetic analysis demonstrated that the first ceftazidime/avibactam-resistant KPC-Kp strain harboured a blaKPC-53 gene in a Tn4401 transposon moved from IncFII(K) to a 43 kb IncX3 plasmid, while a imipenem/cilastatin/relebactam-resistant strain exhibited two copies of the Tn4401 transposon in IncFII(K) and IncX3 plasmids, resulting in an increased blaKPC copy number. Of note, frequency analysis demonstrated that imipenem/cilastatin/relebactam-resistant KPC-Kp consisted of mixed subpopulations harbouring blaKPC-40 and blaKPC-53 alleles.

Conclusions
Our results show the in vivo evolution of genetic rearrangement conferring resistance to imipenem/relebactam in a patient with KPC-Kp infection and treated with different ceftazidime/avibactam-based treatments. The rapid development of mutations and the high adaptability of its genome highlight the potential threat of KPC-Kp.
A comparison of indoor and outdoor calf housing systems using automated and manual feeding methods and their effect on calf health, behavior, growth, and labor
Authors: Sinnott Alison M , Bokkers Eddie A M , Murphy John Paul , Kennedy Emer 
Published: March 2022

Abstract
Housing and feeding are integral to calf rearing, and must meet calf needs while remaining functional for the farmer. This study compared health, behavior, growth, and labor requirements of calves housed in groups indoors and fed via an automatic or manual milk feeding system compared to calves manually fed in individual or group hutches outdoors. Seventy-six (49 Holstein Friesian [HF] and 27 HF × Jersey) dairy heifer calves were balanced for birth weight (35.2 ± 4.95 kg), birth date (1 February ± 7.2 d) and breed. The experiment was a randomized block design with four treatments; 1) indoor group housing with automated feeding (IN_AUTO; 12 calves per pen), 2) indoor group housing with manual feeding (IN_MAN; 12 calves per pen), 3) outdoor group hutch with manual feeding (OUT_G_MAN; 8 calves per pen), and 4) outdoor individual hutch with manual feeding (OUT_I_MAN; 6 calves: 1 per pen). Calves in OUT_treatments moved outdoors at 18 d (± 5.9 d). Each treatment was replicated once. Milk allowance increased gradually from 6 to 8 L/day (15% reconstitution rate) with ad libitum fresh water, concentrates, and hay offered from 3 d old. Gradual weaning occurred at 8 wk old. Measurements were divided into period 1; before movement outdoors, and period 2; after movement outdoors. Health was similar among treatments, regardless of period, with the most frequent score being zero (i.e., healthy). Summarized, standing and lying were observed 24.3% and 29.8%, respectively, in OUT_I_MAN calves, compared to 8.0% and 49.1%, for the other systems, which were similar. No difference in bodyweight (BW) existed between treatments, except at weaning where BW was lower for OUT_I_MAN (67.4 ± 2.84 kg) compared to IN_MAN (74.2 ± 2.01 kg), and day 102 where OUT_I_MAN (94.1 ± 2.85 kg) were lighter than IN_AUTO (101.1 ± 2.10 kg) (P = 0.047). Total labor input was greatest for OUT_I_MAN (00:02:02 per calf per day; hh:mm:ss) and least for IN_AUTO (00:00:21 per calf per day) (P &lt; 0.001). The labor for feeding (00:00:29 per calf per day), feeding inspection (00:00:10 per calf per day), and cleaning equipment (00:00:30 per calf per day) was greatest for OUT_I_MAN. All calves showed good health and growth patterns. Differences in behavior expressed by calves in the OUT_I_MAN, compared to other treatments may indicate compromised welfare. Thus, although outdoor group hutches do not negatively impact calves, indoor housing, particularly using automated feeders, can improve labor efficiency.
Full-fat field cricket (Gryllus bimaculatus) as a substitute for fish meal and soybean meal for weaning piglets: effects on growth performance, intestinal health, and redox status
Authors: Boontiam Waewaree , Hong Jinsu , Kitipongpysan Sumetee , Wattanachai Suchat 
Published: March 2022

Abstract
Full-fat field cricket meal (FCP) is an alternative protein ingredient in livestock production; however, the effects of replacing conventional protein sources with FCP in nursery diets have not been determined. In this study, the effects of the partial replacement of either fish meal or soybean meal with FCP on weaning pigs were evaluated, including the analyses of growth performance, nutrient utilization, intestinal morphology, &#x2028;immunity, oxidative stress, and fecal microbial counts. A total of 100 crossbred weaning pigs [(Landrace × Large White) × Duroc] were allotted to one of the following five treatments with five replicates (four pigs/pen) and fed for 28 d postweaning. Treatments were 1) a corn-soybean meal (SBM)-based diet with 5% fish meal (Positive control; PC), 2) a corn-SBM-based diet without fish meal (Negative control; NC), 3) field crickets replacing fishmeal on a total Lys basis (FCP1), 4) field crickets replacing fishmeal on a kg/kg basis (FCP2), and 5) field crickets replacing fish meal and soybean meal (FCP3). The piglets on FCP1 had a higher body weight on days 14 and 28, and an increased average daily gain over the experimental period than NC (P &lt; 0.05); FCP2 and FCP3 were similar to the FCP1 treatment. The incidence of diarrhea was lower under an FCP-supplemented diet than under the NC diet throughout the study (P &lt; 0.05). Pigs fed FCP1 and FCP2 had a higher digestibility of crude &#x2028;protein (P = 0.041), and all FCP groups increased crude fat digestibility (P = 0.024). FCP1 and FCP2 also increased jejunal villus height &#x2028;(P = 0.009), whereas the increase in jejunal villus-to-crypt ratios (P = 0.019) was greater in pigs fed the FCP2 diet than those fed the NC diet. Furthermore, FCP2 supplementation increased serum immunoglobulin A levels on days 14 and 28, including reduced serum interleukin-6 and tumor necrosis factor alpha levels (P &lt; 0.05). Pigs fed an FCP2 diet had reduced malondialdehyde levels than those fed a PC diet, while pigs fed an FCP2 diet had higher superoxide dismutase and glutathione peroxidase levels, and more fecal Lactobacillus spp. than those fed an NC diet (P &lt; 0.05). These results support the use of FCP as an alternative protein ingredient with beneficial effects on growth performance, intestinal morphology, antioxidant capacity, and intestinal microbiota. In particular, FCP can be used as a partial substitute for fish meal and soybean meal without detrimental effects on weaning pigs.
Comparison of Work- and Nonwork-Related Lower Extremity Burn Injuries: A Retrospective Analysis
Authors: Gurbuz Kayhan , Demir Mete 
Published: March 2022

Abstract
Lower extremity burn injuries are among the most affected anatomical regions in hospitalized burn patients. Our objective was to compare burn patients with work-related isolated lower extremity burn injuries (w-ILEBI) and patients with nonwork-related isolated lower extremity burn injuries (nw-ILEBI). Sixty-four (15.7%) of 407 patients (16–92 years) were in the w-ILEBI cohort. The most extensive burn wounds were among patients in the fire-flame group with a median total body surface area percent (%TBSA) of 27.0 (interquartile range = 11.0%–45.0%). While 50 (76.9%) patients in the electrical group had full-thickness burns, 99 (60%) of the cases in the scald group had superficial partial-thickness burns. Blood and wound sample cultures were positive in 42 (29.4%) patients in the fire-flame group. Approximately one-third of patients in the fire-flame/electrical group required escharotomy/fasciotomy procedures, only one (0.6%) case in the scald group, and none in the chemical/contact groups. Thirty-three (51%) of the patients in the electrical group underwent skin grafting, but 14 (21.5%) required amputations. The highest mortality was detected in 27 (18.9%) patients in the fire-flame group. This study revealed several differences in clinical characteristics of isolated lower extremity burn injuries (ILEBI): burn mechanism and depth, total body surface area percent (%TBSA), infection, surgery requirements, laterality, and mortality. Within the scope of occupational health and safety measures, protective clothing and increased workplace and safety training for employers/employees should be implemented.
Outcomes of nivolumab monotherapy for previously treated metastatic renal cell carcinoma: a real-world multi-institution data with a minimum of 2 years of follow-up
Authors: Ishihara Hiroki , Nemoto Yuki , Tachibana Hidekazu , Fukuda Hironori , Yoshida Kazuhiko , Kobayashi Hirohito , Iizuka Junpei , Hashimoto Yasunobu , Takagi Toshio , Ishida Hideki , Kondo Tsunenori , Tanabe Kazunari 
Published: March 2022

Abstract

Objectives
To investigate the long-term follow-up outcomes of nivolumab monotherapy for previously treated metastatic renal cell carcinoma, using real-world data.

Methods
A total of 121 patients were treated with nivolumab monotherapy as subsequent therapy after the failure of prior tyrosine kinase inhibitor therapy between January 2013 and December 2021 at four affiliated institutions. To evaluate the outcome after 2 years or more, we selected patients in whom nivolumab therapy was started in December 2019 or earlier because data collection was performed until the end of December 2021.

Results
Seventy-four patients were evaluated. During the median follow-up period of 25.8 months, 62 (84%) and 40 (54%) patients had disease progression and died, respectively. Nivolumab was administered as second-line therapy in 43 patients (58%). The median progression-free survival and overall survival were 5.52 and 31.1 months, respectively, and objective response rate was 36%. There was no difference in progression-free survival or overall survival based on the treatment line of nivolumab (P = 0.915, P = 0.559). The magnitude of tumor response and development of immune-related adverse events were significantly associated with progression-free survival (P &lt; 0.0001, P &lt; 0.0001, respectively) and overall survival (P &lt; 0.0001, P = 0.0002, respectively). Treatment-related adverse events developed in 38 patients (51%), including 33 (45%) who had immune-related adverse events. Steroid administration was needed in nine patients (12%).

Conclusions
The present real-world multi-institution study with long-term follow-up data demonstrates that nivolumab monotherapy is effective for previously treated metastatic renal cell carcinoma, prolonging survival, improving tumor response and has a manageable safety profile.
Seasonality of Ticks and Prevalence of Rickettsiae Species in Dermacentor variabilis and Amblyomma maculatum Across Oklahoma Pastures
Authors: Noden Bruce H , Dubie Trisha R , Henriquez Brandon E , Gilliland Meghan , Talley Justin L 
Published: March 2022

Abstract
Tick-borne diseases are an increasing concern for people and companion animals in the United States, but there is a need for continued vigilance regarding livestock in pasture systems. The south-central United States has some of the highest incidences of tick-borne diseases, and there is a need to re-examine the ecology of tick vectors in relation to pasture systems and livestock. The objective of this study was to establish a baseline of seasonal activity for tick species in diverse regional Oklahoma pastures and screen for important pathogens in Dermacentor variabilis (Say) and Amblyomma maculatum Koch group that may impact livestock and human health. Between 2015 and 2017, transects in five pastures across Oklahoma were visited each month. DNA extracted from adult D. variabilis and A. maculatum group was tested for the presence of bacterial pathogens. We found that tick communities in pastures across Oklahoma differ by season, abundance, and bacterial presence and prevalence. The peak abundance of Amblyomma americanum (L.) adults and nymphs occurred a month earlier over the 2 yr of the study compared with historical studies in the same regions. Additionally, we observed notable differences in peak activity between A. americanum adults and nymphs collected in pastures in central Oklahoma (April) versus pastures in northern part of the state (May). We detected Rickettsia parkeri, R. bellii, and Anaplasma sp. DNA in D. variabilis from pastures across the state. These results potentially have important ramifications for human and livestock risk of encountering infected ticks in pastures across the southern Great Plains.
Surgical treatment of ductal biliary recurrence of poorly cohesive gastric cancer mimicking primary biliary tract cancer: a case report
Authors: Poletto Edoardo , Ruzzenente Andrea , Turri Giulia , Conci Simone , Ammendola Serena , Luchini Claudio , Scarpa Aldo , Guglielmi Alfredo 
Published: March 2022

Abstract
Ductal biliary recurrence of cancers arising in other anatomical districts is a rare event, usually observed in the setting of disseminated disease; hence surgery is rarely a viable option. We present the case of a 56-year-old male who underwent subtotal gastric resection 7 years earlier for a poorly cohesive gastric cancer, presenting with obstructive jaundice. Magnetic resonance imaging and computed tomography scan suggested primary malignant obstruction of the main bile duct. Percutaneous transhepatic biliary drainage was performed to palliate jaundice and obtain biopsies; pathological examination suggested a ductal biliary recurrence of gastric carcinoma. Pancreaticoduodenectomy and bile duct resection were performed. Histology, immunohistochemistry and molecular profiling confirmed that the stenosis represented a gastric cancer metastasis. This is the first case of an isolated ductal biliary recurrence of gastric cancer amenable to surgical resection. This clinical case suggests that biliary obstructions in patients with previous oncological history require biliary biopsies to exclude a recurrent disease.
Acute appendicitis with associated trichobezoar of feline hair
Authors: Redden Mark , Ghadiri Marjan 
Published: March 2022

Abstract
A trichobezoar is an accumulation of ingested hair that forms a mass within the gastro-intestinal tract. Thrichobezoars usually consist of human hair and are known to cause obstruction and even perforation of gastrointestinal organs. There have been approximately seven reported cases of acute appendicitis with association trichobezoars found at the time of appendicectomy. We report a unique case of acute appendicitis with an associated trichobezoar of feline hair. A 15-year-old male presented with a 24-hour history of abdominal pain. A computed tomography scan demonstrated features of appendicitis with several hyperdensities within the base of the appendix. At the time of appendicectomy, the appendix was found to be perforated at the base. Faecoliths were identified containing numerous short, light-coloured hairs. Following the procedure, the family confirmed that they have a pet cat with short, light-coloured hair. The patient had an uneventful recovery.
Getting Accurate about Knowledge
Authors: Carter Sam , Goldstein Simon 
Published: March 2022

AbstractThere is a large literature exploring how accuracy constrains rational degrees of belief. This paper turns to the unexplored question of how accuracy constrains knowledge. We begin by introducing a simple hypothesis: increases in the accuracy of an agent’s evidence never lead to decreases in what the agent knows. We explore various precise formulations of this principle, consider arguments in its favour, and explain how it interacts with different conceptions of evidence and accuracy. As we show, the principle has some noteworthy consequences for the wider theory of knowledge. First, it implies that an agent cannot be justified in believing a set of mutually inconsistent claims. Second, it implies the existence of a kind of epistemic blindspot: it is not possible to know that one’s evidence is misleading.