bluuebunny/crossref_metadata_2025_split huggingface dataset:
On airborne tire wear particles along roads with different traffic characteristics using passive sampling and optical microscopy, single particle SEM/EDX, and µ-ATR-FTIR analyses
Authors: Gao Zhiqiang , Cizdziel James V. , Wontor Kendall , Clisham Carly , Focia Kaylea , Rausch Juanita , Jaramillo-Vogel David 
Published: October 2022

Tire wear particles (TWPs) are a major category of microplastic pollution produced by friction between tires and road surfaces. This non-exhaust particulate matter (PM) is transported through the air and with runoff leading to environmental pollution and health concerns. Here, we collected airborne PM along paved roads with different traffic volumes and speeds using Sigma-2 passive samplers. Particles entering the samplers deposit onto substrates for analysis, or, as we modified it, directly into small (60 ml) separatory funnels, which is particularly useful with high particle loads, where a density separation aids in isolating the microplastics. We quantified putative TWPs (∼10–80 µm) deposited on the substrates (primarily adhesive tape on glass slides) and in the funnels using stereomicroscopy. Putative TWP deposition rates (particles/cm2/day ± SD) at 5 m from the road were highest near a busy highway (324 ± 129), followed by a boulevard with moderate traffic (184 ± 93), and a slow traffic avenue (29 ± 7). We observed that deposition rates increased within proximity to the highway: 99 ± 54, 180 ± 88, and 340 ± 145 at 30, 15, and 5 m, respectively. We show that TWP abundances (i.e., deposition and mass concentration) increase with vehicle braking (driving behavior). We observed no differences (p &gt; 0.05) between the separatory funnel and adhesive tape collection methods. In addition, we were able to obtain FTIR spectra of TWPs (&gt;10 µm) using µ-ATR-FTIR. Both deserve further scrutiny as novel sampling and analytical approaches. In a separate sampling campaign, we differentiated 1438 particles (∼1–80 µm) deposited on boron substrates into TWP, metal, mineral, and biogenic/organic classes with single particle SEM/EDX analysis based on morpho-textural-chemical classification and machine learning. The results revealed similar concentration trends with traffic (high &gt; moderate &gt; low), with the distribution of particle sources alike for the highway and the moderate road: TWPs (∼38–39%) &gt; biogenic (∼34–35%) &gt; minerals (∼23–26%), and metallic particles (∼2–3%). The low traffic road yielded a much different distribution: biogenic (65%) &gt; minerals (27%) &gt; TWPs (7%) &gt; metallic particles (1%). Overall, this work provides much-needed empirical data on airborne TWPs along different types of roads.
Land use change and its effect on ecosystem services in an Oxisol of the eastern High Plains of meta department in Colombia
Authors: Moreno-Conn Lina M. , Rodríguez-Hernandez Nubia S. , Arguello José Orlando , Gallo Gordillo Oscar , Bernal-Riobo Jaime H. , Arango Miguel , Baquero José E. 
Published: October 2022

Increasing cultivation on acid soils of Colombia’s Eastern High Plains in the Orinoquia region require crop management strategies that balance commercial and environmental interests. Previous research showed the effects of land preparation and management on certain aspects of ecosystem services of these acid soils. This research aimed to comprehensively evaluate the impact of use and soil management in a native savanna Oxisol converted to five production systems gaining traction among local farmers: Annual crops rotation–sugarcane (ACR-S); Annual crops rotation-maize-soybean (ACR-MS); Sugarcane monocrop (SM); Rubber associated with Desmodium ovalifollium (R-Do) as a cover crop; Pasture Brachiaria decumbens - Annual crops rotation (PBd-ACR) and native savanna (NS) as a control. The experimental design consisted of conditioning the soil with two doses of dolomitic lime and incorporating three tillage methods over 5 years. A Principal Components Analysis (PCA) constructed indicators of ecosystem services (ES), such as fertility, water regulation, macroinvertebrate diversity, climate control related to greenhouse gas emissions (GHG) and carbon stock. The indicator values were adjusted between 0.10 and 1.0. Physicochemical variables with the greatest statistical weight were identified for components 1 and 2 each year. The fertility indicator of the native savanna for year one had a low range with a value of 0.11 compared to the other systems (p ≤ 0.05). During the years two, three, and four, soil fertility increased in the PBd-ACR (0.51) production system compared to the other systems. PBd-ACR generally improved water supply, although with lower indicator values than ACR-MS and R-Do. PBd-ACR show high biodiversity for edaphic macroinvertebrates compared to other systems. Field measurements detected methane emissions only in the SM system. Oxisols changed with the introduction of crops. The positive and negative tradeoffs of production systems and their agronomic practices should be considered in agricultural development of these acid soils.
Impact of soil types and root exudates on cadmium and petroleum hydrocarbon phytoremediation by Sorghum sudanense, Festuca arundinace, and Lolium perenne
Authors: He Mengfan , Li Zhongbao , Chen Cheng , Mei Ping 
Published: October 2022

With the development of industrialization, soils around the world have been polluted by heavy metals and oil to different degrees in recent years, and soil remediation has become a global problem. Phytoremediation has a wide application prospect because of its environmental friendliness and easy availability of materials.ObjectiveTo explore the effects of soil types and root exudates on the removal of cadmium and petroleum hydrocarbon in soils.MethodA pot experiments with three soil types (sandy, loamy and clayey) of the Changning-Weiyuan area of Sichuan province and three root exudates (citric acid, glycine, and maltose) were carried out using Sorghum sudanense (Piper) Stapf., Lolium perenne L., and Festuca arundinacea L. as test materials. Plants were grown in soils contaminated by cadmium and petroleum at different concentrations.ResultThe biomass of S. sudanense, the translocation ratio and removal rate of cadmium in S. sudanense decreased gradually with increasing soil cadmium concentration. The promotion effects of the three root exudates on S. sudanense were in the following order: citric acid &gt; glycine &gt; maltose. At the same cadmium pollution conditions, the biomass levels of S. sudanense in sandy, loamy, and clayey soils were in the following order: clayey soil &gt; loamy soil &gt; sandy soil. On the contrary, the concentration, translocation ratio, and removal rate of cadmium in S. sudanense grown in the different soils treated with root exudates were in the following order: sandy soil &gt; loamy soil &gt; clayey soil. Under the three soil conditions, the fresh weight of F. arundinacea (0.36 ~ 0.68 g) and S. sudanense (0.51 ~ 0.99 g) increased significantly (p &lt; 0.05). The total petroleum hydrocarbons degradation efficiencies of F. arundinacea, L. perenne, and S. sudanense were high in sandy soil (34.27% ~ 60.52%). Changing the type of root exudate had a significant impact on the degradation of total petroleum hydrocarbons in sandy soil (p &lt; 0.05).ConclusionThis study showed that soil types impacted the accumulation of cadmium and petroleum in plants. Phytoremediation of cadmium and petroleum contaminated soil could be enhanced by the application of root exudates. This study recommend that the method is suitable for field remediation of soils contaminated with mild cadmium and petroleum hydrocarbons.
Comprehensive analysis of transient receptor potential channels-related signature for prognosis, tumor immune microenvironment, and treatment response of colorectal cancer
Authors: Wang Lei , Chen Xingte , Zhang Hejun , Hong Liang , Wang Jianchao , Shao Lingdong , Chen Gang , Wu Junxin 
Published: October 2022

BackgroundTransient receptor potential channels (TRPC) play critical regulatory functions in cancer occurrence and progression. However, knowledge on its role in colorectal cancer (CRC) is limited. In addition, neoadjuvant treatment and immune checkpoint inhibitors (ICIs) have increasing roles in CRC management, but not all patients benefit from them. In this study, a TRPC related signature (TRPCRS) was constructed for prognosis, tumor immune microenvironment (TIME), and treatment response of CRC.MethodsData on CRC gene expression and clinical features were retrospectively collected from TCGA and GEO databases. Twenty-eight TRPC regulators (TRPCR) were retrieved using gene set enrichment analysis. Different TRPCR expression patterns were identified using non-negative matrix factorization for consensus clustering, and a TRPCRS was established using LASSO. The potential value of TRPCRS was assessed using functional enrichment analysis, tumor immune analysis, tumor somatic mutation analysis, and response to preoperative chemoradiotherapy or ICIs. Moreover, an external validation was conducted using rectal cancer samples that received preoperative chemoradiotherapy at Fujian Cancer Hospital (FJCH)viaqRT-PCR.ResultsAmong 834 CRC samples in the TCGA and meta-GEO cohorts, two TRPCR expression patterns were identified, which were associated with various immune infiltrations. In addition, 266 intersected genes from 5564 differentially expressed genes (DEGs) between two TRPC subtypes, 4605 DEGs between tumor tissue and adjacent non-tumor tissue (all FDR&lt; 0.05, adjusted P&lt; 0.001), and 1329 prognostic related genes (P&lt; 0.05) were identified to establish the TRPCRS, which was confirmed in the TCGA cohort, two cohorts from GEO, and one qRT-PCR cohort from FJCH. According to the current signature, the high-TRPC score group had higher expressions of PD-1, PD-L1, and CTLA4, lower TIDE score, and improved response to anti-PD-1 treatment with better predictive ability. Compared to the high-TRPC score group, the low-TRPC score group comprised an immunosuppressive phenotype with increased infiltration of neutrophils and activated MAPK signaling pathway, but was more sensitive to preoperative chemoradiotherapy and associated with improved prognosisConclusionsThe current TRPCRS predicted the prognosis of CRC, evaluated the TIME in CRC, and anticipated the response to immune therapy and neoadjuvant treatment.
Use of the NIH consensus criteria in cellular and soluble biomarker research in chronic graft-versus-host disease: A systematic review
Authors: Milosevic Emina , Babic Antonija , Iovino Lorenzo , Markovic Milos , Grce Magdalena , Greinix Hildegard 
Published: October 2022

ObjectivesChronic graft-versus-host disease (cGvHD) is the most frequent cause of late non-relapse mortality after allogeneic haematopoietic stem cell transplantation (alloHCT). Nevertheless, established biomarkers of cGvHD are still missing. The National Institutes of Health (NIH) Consensus Development Project on Criteria for Clinical Trials in cGvHD provided recommendations for biomarker research. We evaluated to which extent studies on cellular and soluble biomarkers in cGvHD published in the last 10 years complied with these recommendations. Also, we highlight the most promising biomarker candidates, verified in independent cohorts and/or repeatedly identified by separate studies.MethodsWe searched Medline and EMBASE for “cGvHD”, “biomarkers”, “soluble” and “cells” as MeSH terms or emtree subject headings, and their variations on July 28th, 2021, limited to human subjects, English language and last ten years. Reviews, case reports, conference abstracts and single nucleotide polymorphism studies were excluded. Criteria based on the set of recommendations from the NIH group for biomarker research in cGvHD were used for scoring and ranking the references.ResultsA total of 91 references encompassing 15,089 participants were included, 54 prospective, 17 retrospective, 18 cross-sectional, and 2 studies included both prospective and retrospective cohorts. Thirty-five papers included time-matched controls without cGvHD and 20 studies did not have any control subjects. Only 9 studies were randomized, and 8 were multicentric. Test and verification cohorts were included in 11 studies. Predominantly, diagnostic biomarkers were explored (n=54). Assigned scores ranged from 5-34. None of the studies fulfilled all 24 criteria (48 points). Nevertheless, the scores improved during the last years. Three cell subsets (CXCR3+CD56bright NK cells, CD19+CD21low and BAFF/CD19+ B cells) and several soluble factors (BAFF, IL-15, CD163, DKK3, CXCL10 and the panel of ST2, CXCL9, MMP3 and OPN) had the highest potential as diagnostic and/or prognostic biomarkers in cGvHD.ConclusionDespite several limitations of this review (limited applicability for paediatric population, definition of verification, missing data on comorbidities), we identified promising candidate biomarkers for further evaluation in multicentre collaborative studies. This review confirms the importance of the NIH consensus group criteria for improving the quality and reproducibility of cGvHD biomarker research.
MUC22, HLA-A, and HLA-DOB variants and COVID-19 in resilient super-agers from Brazil
Authors: Castelli Erick C. , de Castro Mateus V. , Naslavsky Michel S. , Scliar Marilia O. , Silva Nayane S. B. , Pereira Raphaela N. , Ciriaco Viviane A. O. , Castro Camila F. B. , Mendes-Junior Celso T. , Silveira Etiele de S. , de Oliveira Iuri M. , Antonio Eduardo C. , Vieira Gustavo F. , Meyer Diogo , Nunes Kelly , Matos Larissa R. B. , Silva Monize V. R. , Wang Jaqueline Y. T. , Esposito Joyce , Cória Vivian R. , Magawa Jhosiene Y. , Santos Keity S. , Cunha-Neto Edecio , Kalil Jorge , Bortolin Raul H. , Hirata Mário Hiroyuki , Dell’Aquila Luiz P. , Razuk-Filho Alvaro , Batista-Júnior Pedro B. , Duarte-Neto Amaro N. , Dolhnikoff Marisa , Saldiva Paulo H. N. , Passos-Bueno Maria Rita , Zatz Mayana 
Published: October 2022

BackgroundAlthough aging correlates with a worse prognosis for Covid-19, super elderly still unvaccinated individuals presenting mild or no symptoms have been reported worldwide. Most of the reported genetic variants responsible for increased disease susceptibility are associated with immune response, involving type I IFN immunity and modulation; HLA cluster genes; inflammasome activation; genes of interleukins; and chemokines receptors. On the other hand, little is known about the resistance mechanisms against SARS-CoV-2 infection. Here, we addressed polymorphisms in the MHC region associated with Covid-19 outcome in super elderly resilient patients as compared to younger patients with a severe outcome.MethodsSARS-CoV-2 infection was confirmed by RT-PCR test. Aiming to identify candidate genes associated with host resistance, we investigated 87 individuals older than 90 years who recovered from Covid-19 with mild symptoms or who remained asymptomatic following positive test for SARS-CoV-2 as compared to 55 individuals younger than 60 years who had a severe disease or died due to Covid-19, as well as to the general elderly population from the same city. Whole-exome sequencing and an in-depth analysis of the MHC region was performed. All samples were collected in early 2020 and before the local vaccination programs started.ResultsWe found that the resilient super elderly group displayed a higher frequency of some missense variants in the MUC22 gene (a member of the mucins’ family) as one of the strongest signals in the MHC region as compared to the severe Covid-19 group and the general elderly control population. For example, the missense variant rs62399430 at MUC22 is two times more frequent among the resilient super elderly (p = 0.00002, OR = 2.24).ConclusionSince the pro-inflammatory basal state in the elderly may enhance the susceptibility to severe Covid-19, we hypothesized that MUC22 might play an important protective role against severe Covid-19, by reducing overactive immune responses in the senior population.
Biochemical and genomic identification of novel biomarkers in progressive sarcoidosis: HBEGF, eNAMPT, and ANG-2
Authors: Casanova Nancy G. , Reyes-Hernon Vivian , Gregory Taylor , Sun Belinda , Bermudez Tadeo , Hufford Matthew K. , Oita Radu C. , Camp Sara M. , Hernandez-Molina Gabriela , Serrano Jorge Rojas , Sun Xiaoguang , Fimbres Jocelyn , Mirsaeidi Mehdi , Sammani Saad , Bime Christian , Garcia Joe G. N. 
Published: October 2022

BackgroundProgressive pulmonary fibrosis is a serious complication in subjects with sarcoidosis. The absence of reliable, non-invasive biomarkers that detect early progression exacerbates the difficulty in predicting sarcoidosis severity. To potentially address this unmet need, we evaluated a panel of markers for an association with sarcoidosis progression (HBEGF, NAMPT, IL1-RA, IL-6, IL-8, ANG-2). This panel encompasses proteins related to inflammation, vascular injury, cell proliferation, and fibroblast mitogenesis processes.MethodsPlasma biomarker levels and biomarker protein expression in lung and lymph nodes tissues (immunohistochemical studies) from sarcoidosis subjects with limited disease and progressive (complicated) sarcoidosis were performed. Gene expression of the protein-coding genes included in this panel was analyzed using RNAseq in sarcoidosis granulomatous tissues from lung and lymph nodes.ResultsExcept for IL-8, plasma levels of each biomarker—eNAMPT, IL-1RA, IL-6, ANG-2, and HBEGF—were significantly elevated in sarcoidosis subjects compared to controls. In addition, plasma levels of HBEGF were elevated in complicated sarcoidosis, while eNAMPT and ANG-2 were observed to serve as markers of lung fibrosis in a subgroup of complicated sarcoidosis. Genomic studies corroborated HBEGF and NAMPT among the top dysregulated genes and identified cytokine-related and fibrotic pathways in lung granulomatous tissues from sarcoidosis.ConclusionThese findings suggest HBEGF, eNAMPT, and ANG-2 may serve as potential novel indicators of the clinical severity of sarcoidosis disease.
Abnormal dynamic functional network connectivity in male obstructive sleep apnea with mild cognitive impairment: A data-driven functional magnetic resonance imaging study
Authors: Li Haijun , Li Lan , Li Kunyao , Li Panmei , Xie Wei , Zeng Yaping , Kong Linghong , Long Ting , Huang Ling , Liu Xiang , Shu Yongqiang , Zeng Li , Peng Dechang 
Published: October 2022

ObjectiveThe purpose of this study was to investigate the dynamic functional network connectivity (FNC) and its relationship with cognitive function in obstructive sleep apnea (OSA) patients from normal cognition (OSA-NC) to mild cognitive impairment (OSA-MCI).Materials and methodsEighty-two male OSA patients and 48 male healthy controls (HC) were included in this study. OSA patients were classified to OSA-MCI (n = 41) and OSA-NC (n = 41) based on cognitive assessments. The independent component analysis was used to determine resting-state functional networks. Then, a sliding-window approach was used to construct the dynamic FNC, and differences in temporal properties of dynamic FNC and functional connectivity strength were compared between OSA patients and the HC. Furthermore, the relationship between temporal properties and clinical assessments were analyzed in OSA patients.ResultsTwo different connectivity states were identified, namely, State I with stronger connectivity and lower frequency, and State II with lower connectivity and relatively higher frequency. Compared to HC, OSA patients had a longer mean dwell time and higher fractional window in stronger connectivity State I, and opposite result were found in State II, which was mainly reflected in OSA-MCI patients. The number of transitions was an increasing trend and positively correlated with cognitive assessment in OSA-MCI patients. Compared with HC, OSA patients showed extensive abnormal functional connectivity in stronger connected State I and less reduced functional connectivity in lower connected State II, which were mainly located in the salience network, default mode network, and executive control network.ConclusionOur study found that OSA patients showed abnormal dynamic FNC properties, which was a continuous trend from HC, and OSA-NC to OSA-MCI, and OSA patients showed abnormal dynamic functional connectivity strength. The number of transformations was associated with cognitive impairment in OSA-MCI patients, which may provide new insights into the neural mechanisms in OSA patients.
Hypoglossal nerve stimulation in adolescents with down syndrome and obstructive sleep apnea: A systematic review and meta-analysis
Authors: Liu Pan , Kong Weiguo , Fang Caijing , Zhu Kangxu , Dai Xiaohua , Meng Xiangming 
Published: October 2022

ObjectiveTo evaluate the efficacy and adverse effects of hypoglossal nerve stimulation in adolescents with down syndrome and obstructive sleep apnea.MethodsA systematic search was conducted using PubMed, Web of Science, Embase, and Scopus databases. The systematic review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. A comprehensive search strategy used a combination of Medical Subject Headings and free words with “OR” and “AND.” Articles were screened to extract data reporting apnea-hypopnea index, quality of life, voltage, follow-up duration, and complications. All included participants were adolescents with down syndrome and obstructive sleep apnea.ResultsA total of 92 articles were identified, of which 9 articles met the inclusion criteria. A total of 106 patients were included. All the studies showed that patients receiving hypoglossal nerve stimulation experienced a significant decrease in apnea-hypopnea index (at least 50%). The pooled AHI was significantly lower in patients following treatment (mean AHI reduction 17.43 events/h, 95% confidence interval 13.98–20.88 events/h, P &lt; 0.001) after 2 case reports were excluded. The pooled OSA-18 were significantly decreased in 88 patients after treatment (mean OSA-18 reduction 1.67, 95% confidence interval 1.27–2.08, P &lt; 0.001) after excluding 5 studies. Four investigations examined the necessity to optimize stimulation voltage for arousal during treatment. The most common complication was pain or discomfort in the tongue or mouth. Most studies had relatively short patient follow-up periods, with the most extended follow-up being 44–58 months.ConclusionHypoglossal nerve stimulation significantly reduces apnea-hypopnea index and improves the quality of life; and thus, could be a potential alternative therapy for obstructive sleep apnea in adolescents with down syndrome. The adolescent's age, potential complications, adverse events, long-term efficacy, and comfort, needs to be considered while performing hypoglossal nerve stimulation.