bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infecção pelo HIV/Aids em população indígena: estudo transversal
Authors: Nunes Daniela Aparecida de Souza , Silva André Soares da , Oliveira Mágda Lúcia Félix de 
Published: February 2022

Objetivo: Descrever aspectos epidemiológicos e a evolução da infecção pelo HIV entre povos indígenas da região sul do estado do Mato Grosso Sul. Método: Estudo transversal, descritivo e retrospectivo, realizado a partir de registros do Sistema de Informação de Agravos de Notificação dos anos 2005-2018. Dados de indivíduos com 13 anos ou mais, da população indígena assistida pelo Distrito Sanitário Especial Indígena do Polo Base de Japorã e diagnóstico de infecção pelo HIV/Aids, foram transferidos para uma planilha de comparação com sistema de informação local. Para o cálculo da prevalência e evolução de casos foi utilizado o número de casos novos anual de HIV/Aids. Resultados: Foram identificados 17 (65,4%) casos em mulheres, 13 (72,2%) entre 20-39 anos. Cinco (19,2%) evoluíram para Aids. As taxas de detecção do HIV/Aids, para os anos de 2016 e 2018 foram de 115.54 e 155.49 respectivamente. As taxas de prevalência do HIV apresentaram flutuações entre os anos 2016, 217 e 218. A aldeia Porto Lindo apresentou uma taxa de detecção de 59,878/100 mil habitantes, Yvykatu 400,802/100 mil habitantes e a Pyelito Kuê 602,409/100 mil habitantes. A taxa de óbito por Aids na aldeia Porto Lindo foi de 2,24/10 mil habitantes e no Yvykatu 40,08/10 mil habitantes. A taxa de abandono para a aldeia Porto Lindo foi de 6,73/10 mil habitantes. Conclusão: As taxas de detecção de HIV apresentaram tendência de crescimento no povo indígena estudado. É necessário que sejam realizados estudos para direcionamento de ações que impactem os indicadores de prevalência da infecção.
Uso de CRISPR-Cas em plasmídeos conjugativos para o controle da disseminação de resistência a antibióticos em Enterococcus: uma revisão de literatura.
Authors: Ribeiro Elias El-Shaddai dos Santos Nery Nunes , Abreu Joel Antônio Cordeiro de , Brandão Fabiana 
Published: February 2022

Justificativa: Há um forte apelo mundial para controle do uso de antimicrobianos associado a novas abordagens terapêuticas contra infecções multirresistentes, como as ocasionados pelas espécies E. faecalis e E. faecium, patógenos oportunistas comensais normalmente presentes em infecções relacionadas à assistência à saúde (IRAS). A elevada capacidade de adaptação desses patógenos ao ambiente nosocomial e a aquisição de genes de resistência e virulência estão entre os principais fatores que contribuem para o agravo dessas infecções. CRISPR-Cas atua como um sistema de defesa bacteriano, selecionado evolutivamente devido à associação de bactérias com vírus, presente em algumas linhagens de E. faecalis e E. faecium. Especula-se que CRISPR-Cas poderiam ser empregados em diferentes vetores, inclusive plasmídeos conjugativos, para edição e inativação de genes de resistência. Objetivos: Investigar o uso de CRISPR-Cas em plasmídeos conjugativos como controle de disseminação de linhagens enterocócicas multirresistentes. Metodologia: Trata-se de revisão baseada em escopo, aplicando critérios de seleção e inclusão de estudos, no intervalo temporal entre 2016 a 2021. Resultados e Considerações finais: Ferramentas de edição molecular, como CRISPR-Cas, podem ser promissoras alternativas no controle de infecções multirresistentes e contribuir no controle de disseminação hospitalar. As linhagens que não apresentam este sistema são mais susceptíveis a aquisição de elementos genéticos móveis, adquirindo genes de resistência e virulência. A aplicabilidade de CRISPR-Cas em plasmídeos conjugativos é um método inovador e factível, capaz de interferir na aquisição de elementos genéticos móveis, e consequentemente, a redução de genes de resistência e virulência, tanto no gênero Enterococcus quanto em outros gêneros.
Nutrição e compostos essenciais e não essenciais na degradação epigenética associada à prevenção e combate ao câncer
Authors: Luz Rogério da , Lima Junior Emilton , Boldt Angelica Beate Winter 
Published: February 2022

Os componentes presentes em alguns alimentos tem a capacidade de influenciar diretamente o processo saúde-doença por meio de mecanismos moleculares epigenéticos, reduzindo o risco de desenvolvimento de algumas doenças, sobretudo as Doenças Crônicas Não Transmissíveis (DCNTs), como o câncer. Nesse sentido, o presente estudo busca elucidar o papel de alguns nutrientes e compostos bioativos dos alimentos na modulação epigenética como forma de prevenção e combate ao câncer. Para tanto, foi realizada uma revisão de escopo nas bases de dados LILACS, PubMed e BVS. Foram utilizados os descritores: Epigenômica, Composto Fitoquímicos, Neoplasias e Nutrientes, todos selecionados de acordo com os Descritores em Ciências da Saúde e utilizados nos idiomas: Português e Inglês. Inicialmente foram encontradas 647 publicações e após a aplicação dos critérios de inclusão e exclusão pré- definidos, restaram 9 publicações pertinentes para construção do trabalho. Verificou-se a partir da literatura selecionada, que alguns nutrientes e compostos bioativos, tais como a Curcumina do açafrão da terra, o Sulforafano e o Isotiocianato de Fenetil (PEITC) dos vegetais crucíferos, o Resveratrol (RES) das uvas vermelhas e do vinho, o β-caroteno dos vegetais alaranjados, a Vitamina C das frutas cítricas e alguns fitoquímicos, como a Quercetina e o Ácido cafeico, desempenham importante papel no combate ao câncer, pois, dentre outros benefícios, eles podem estimular epigenéticamente a expressão gênica de fatores preventivos, como também podem inibir a expressão de fatores ligados à metástase e progressão da patologia já instalada. Por fim, percebe-se que a influência que alguns alimentos tem sobre a expressão gênica representa uma importante ferramenta contra o câncer, sobretudo em relação à prevenção. No entanto, se faz necessário a realização de estudos mais detalhados em relação à doses e possíveis efeitos maléficos.
Isolamento de bactérias Gram-negativas em amostras de sedimento de manguezal em São Luís, Maranhão
Authors: Silva Verônica Duarte da , Fróes Yuri Nascimento , Monteiro Joveliane de Melo , Souza Neemias Muniz de , Lima Neuriane Silva , Sousa Leila Cristina Almeida de , Moura Anna Regina Lanner de , Sousa Joicy Cortez de Sá , Silva Fabrício Brito , Silva Darlan Ferreira da , Monteiro Andrea de Souza , Silva Maria Raimunda Chagas 
Published: February 2022

Os sedimentos dos manguezais são muito úmidos e ricos em matéria orgânica em decomposição, formados por uma série de espécies resistentes ao fluxo das marés. Este ecossistema costeiro é formado por uma série de espécies resistentes ao fluxo das marés, com solo contendo um ambiente rico que limita a diversidade de biomas. Objetivo desse estudo foi a identificação de microrganismos e a caracterização físico-química em amostras de sedimento de dois pontos de um manguezal localizados nas proximidades de um hospital na ilha de São Luís, Maranhão. Duas amostras do sedimento foram coletadas em tubos apropriados, armazenadas e encaminhadas para o Laboratório de Microbiologia Ambiental na Universidade CEUMA. No laboratório, alíquotas foram retiradas para as características físico-químicas utilizando-se metodologia conforme preconiza a Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), rastreando características como granulometria, matéria orgânica, carbono orgânico, nitrato e nitrito, fosforo total e pH. O solo foi caracterizado como orgânico, rico em nitrato, nitrito e sais minerais, com pH (6,3), característico do ecossistema de mangues. Foi realizado isolamento, identificação dos isolados por MALDI-TOF (do inglês, Matrix-assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) e teste de susceptibilidade aos antimicrobianos utilizado metodologia de Kirby-Bauer. Foram identificadas bactérias gram-negativas, principalmente do gênero Ochrobactrum e espécies termotolerante, como Escherichia coli que são consideradas patógenos indicadores de falta de saneamento básico e que apresentou multirresistência in vitro aos antibióticos testados. Nosso estudo fortalece a ideia de mais pesquisas para avaliar a diversidade de microrganismos presentes em manguezais para incentivar e alertar sobre a preservação e controle sanitário de ecossistemas de mangue.
Pengaruh Manajemen Pengetahuan, Budaya Organisasi dan Keterlibatan Kerja Terhadap Kinerja Pegawai (The Effect of Knowledge Management, Organizational Culture and Work Involvement to Employee Performance)
Authors: Safitri Ayi , Palilati Alida , Zaid Sudirman 
Published: February 2022

The objectives of the study are : (1) The effect of knowledge management, organizational culture and work involvement simultaneously have effect on Employees performance at the Secretariat Office of the DPRD of Southeast Sulawesi Province; (2) the effect of partial knowledge management on Employees Performance at the Secretariat Office of the DPRD of Southeast Sulawesi Province; (3) The effect of partial organizational culture on Employees Performance at the Secretariat Office of the DPRD of Southeast Sulawesi Province; (4) The effect of partial Work involvement on Employees Performance at the Secretariat Office of the DPRD of Southeast Sulawesi ProvinceThe research design used was quantitative research. The population of the study were all employees who worked at the Secretariat Office of the DPRD Southeast Sulawesi Province. The analysis used is quantitative analysis using Multiple Linear Regression Analysis.The results of this study found that: (1) knowledge management, organizational culture and work involvement simultaneously have positif and signifikant effect on employee performance at the Secretariat Office of the DPRD Southeast Sulawesi Province; (2) Knowledge management has positip and significant effects on Employees Performance at the Secretariat Office of the DPRD of Southeast Sulawesi Province; (3) Organizational culture has has poositip and significant on Employees Performance at the Secretariat Office of the DPRD of Southeast Sulawesi Province; (4) Work involvement has positif and significant on Employees Performance at the Secretariat Office of the DPRD of Southeast Sulawesi Province
PENGARUH DISIPLIN, KOMITMEN ORGANISASI DAN LINGKUNGAN KERJA TERHADAP KINERJA PERSONIL PADA SATUAN SABHARA POLRES KENDARI
Authors: Muhlis Muhlis , Nurwati Nurwati , Juharsah Juharsah , Tangalayuk Agustinus , Yusuf Abdul Razak , Hartini Hartini 
Published: February 2022

This study aims to examine and analyze the influence of discipline, organizational commitment and work environment on the performance of personnel at the Kendari Police Sabhara Unit. This research is a quantitative approach. The population in this study was the personnel at the Kendari Police Sabhara Unit with a sample of 56 people. The analysis used multiple regression analysis with the help of IBM SPSS 22 Software. The results showed that (1) Discipline had a positive and significant effect on Personnel Performance at the Kendari Police Sabhara Unit, (2) Organizational Commitment had a positive and significant impact on Personnel Performance at the Sabhara Polres Unit. Kendari, and (3) the work environment has a positive and significant effect on the performance of the personnel in the Kendari Police Sabhara Unit.
Clinical Nomogram for Predicting the Prognosis of Patients With Pulmonary Venous Obstruction After Total Anomalous Pulmonary Venous Connection Repair
Authors: Chen Ling , Qiu Zhihuang , Xu Fan , Chen Xingfeng , Chen Liangwan 
Published: February 2022

BackgroundThe aim of this study was to establish a nomogram to quantify the risk of postoperative pulmonary venous obstruction (PVO) and to make a scientific decision through the decision curve.MethodsIn total, 151 PVO patients with total anomalous pulmonary venous connection (TAPVC) repair in our hospital from December 2008 to December 2015 were involved in this study. A nomogram was generated based on the contribution weights of variables, which were found out by logistic analysis. The optimal clinical decision point was determined by the decision analysis and clinical impact curve, which could assess the net benefit between the nomogram and each independent risk factor for postoperative PVO.ResultPulmonary venous obstruction with TAPVC repair was found to be positively and independently correlated with preoperative pulmonary hypertension, surgical methods, and preoperative pulmonary venous stenosis.Conclusion(s)The study introduced a novel model to aid in clinical decisions making for the patients with TAPVC individually, which may shed light on the evaluation of PVO risk.
Differential Expression Profile of microRNAs and Tight Junction in the Lung Tissues of Rat With Mitomycin-C-Induced Pulmonary Veno-Occlusive Disease
Authors: Song Qing , Chen Ping , Wu Shang-Jie , Chen Yan , Zhang Yan 
Published: February 2022

BackgroundPulmonary veno-occlusive disease (PVOD) is characterized by increased pulmonary vascular resistance. Currently, there is a lack of effective treatment. It is of great significance to explore molecular targets for treatment. This study investigated the differential expression profile of miRNAs and tight junction in the lung tissues of rats with mitomycin-C (MMC)-induced PVOD.MethodsA total of 14 rats were divided into the control group and he PVOD group. We measured mean pulmonary arterial pressure (mPAP) and right ventricular hypertrophy index (RVHI). Pathological changes including those in lung tissues, pulmonary venules, and capillary were detected by H&amp;E and orcein staining. Western blot was used to detect GCN2, ZO-1, occludin, and claudin-5 expression. We analyzed the miRNAs profile in the rat lung tissues by high-throughput sequencing. The top differentially expressed miRNAs were validated by using real-time polymerase chain reaction (RT-PCR).ResultsThere were severe pulmonary artery hypertrophy/hyperplasia, thickening, and occlusion in the small pulmonary veins, pulmonary edema, and dilated capillaries in MMC-induced rats with PVOD. In addition, mPAP and RVHI were significantly increased (P &lt; 0.05). The expression of GCN2 was significantly decreased (P &lt; 0.05). A total of 106 differentially expressed miRNAs were identified. According to the fold changes, the top ten upregulated miRNAs were miRNA-543-3p, miRNA-802-5p, miRNA-493-3p, miRNA-539-3p, miRNA-495, miRNA-380-5p, miRNA-214-5p, miRNA-539-5p, miRNA-190a-3p, and miRNA-431. The top 10 downregulated miRNAs were miRNA-201-3p, miRNA-141-3p, miRNA-1912-3p, miRNA-500-5p, miRNA-3585-5p, miRNA-448-3p, miRNA-509-5p, miRNA-3585-3p, miRNA-449c-5p, and miRNA-509-3p. RT-PCR confirmed that miRNA-214-5p was upregulated, while miRNA-141-3p was downregulated (P &lt; 0.05). Functional analysis showed various signaling pathways and metabolic processes, such as fatty acid biosynthesis, tight junction, and the mTOR signaling pathway. In addition, the expression of the tight junction-related protein of ZO-1, occludin, and claudin-5 was significantly decreased in rats with PVOD (P &lt; 0.05).ConclusionmiRNAs may be involved in the pathogenesis of PVOD. Furthermore, ZO-1, occludin, and claudin-5 verification confirmed that the tight junction may be involved in the development of the disease.
Atrial Fibrillation and Depression: A Bibliometric Analysis From 2001 to 2021
Authors: Ai Yuzhen , Xing Yaxuan , Yan Longmei , Ma Dan , Gao Anran , Xu Qiwu , Zhang Shan , Mao Ting , Pan Qiu , Ma Xiaojuan , Zhang Jingchun 
Published: February 2022

BackgroundThe control of diseases related to atrial fibrillation (AF) may reduce the occurrence of AF, delay progression, and reduce complications, which is beneficial to the prevention and treatment of AF. An increasing number of studies have shown that AF is associated with depression. However, to date, there has not been a bibliometric analysis to examine this field systematically. Our study aimed to visualize the publications to determine the hotspots and frontiers in research on AF and depression and provide guidance and reference for further study.MethodsPublications about AF and depression between 2001 and 2021 were retrieved from the Web of Science Core Collection (WOSCC) database. CiteSpace 5.8. R1, VOSviewer 1.6.16, and Excel 2019 software tools were used to conduct this bibliometric study.ResultsIn total, 159 articles and reviews were analyzed. The number of publications has been increased sharply since 2018. David D. McManus had the largest number of publications. The most prolific country was the USA with 54 publications but the centrality was &lt;0.1. The most prolific institution was Northeastern University. Three clusters were formed based on keywords: The first cluster was composed of atrial fibrillation, depression, anxiety, symptoms, ablation, and quality of life, et al. The second cluster were risk, prevalence, mortality, heart failure, association, et al. While the third cluster included anticoagulation, impact, stroke, management, warfarin, et al. After 2019, stroke and prediction are the keywords with strongest citation bursts.ConclusionResearch on AF and depression is in its infancy. Cooperation and exchanges between countries and institutions must be strengthened in the future. The effect of depression on prevalence and mortality in AF, depression on ablation in AF, and impact of depression on anticoagulation treatment in AF have been the focus of current research. Stroke prevention (including anticoagulant therapy) is the research frontier, which may still be the focus of research in the future.
Investigation of the Utility of Features in a Clinical De-identification Model: A Demonstration Using EHR Pathology Reports for Advanced NSCLC Patients
Authors: Paul Tanmoy , Rana Md Kamruz Zaman , Tautam Preethi Aishwarya , Kotapati Teja Venkat Pavan , Jampani Yaswitha , Singh Nitesh , Islam Humayera , Mandhadi Vasanthi , Sharma Vishakha , Barnes Michael , Hammer Richard D. , Mosa Abu Saleh Mohammad 
Published: February 2022

BackgroundElectronic health record (EHR) systems contain a large volume of texts, including visit notes, discharge summaries, and various reports. To protect the confidentiality of patients, these records often need to be fully de-identified before circulating for secondary use. Machine learning (ML) based named entity recognition (NER) model has emerged as a popular technique of automatic de-identification.ObjectiveThe performance of a machine learning model highly depends on the selection of appropriate features. The objective of this study was to investigate the usability of multiple features in building a conditional random field (CRF) based clinical de-identification NER model.MethodsUsing open-source natural language processing (NLP) toolkits, we annotated protected health information (PHI) in 1,500 pathology reports and built supervised NER models using multiple features and their combinations. We further investigated the dependency of a model's performance on the size of training data.ResultsAmong the 10 feature extractors explored in this study, n-gram, prefix–suffix, word embedding, and word shape performed the best. A model using combination of these four feature sets yielded precision, recall, and F1-score for each PHI as follows: NAME (0.80; 0.79; 0.80), LOCATION (0.85; 0.83; 0.84), DATE (0.86; 0.79; 0.82), HOSPITAL (0.96; 0.93; 0.95), ID (0.99; 0.82; 0.90), and INITIALS (0.97; 0.49; 0.65). We also found that the model's performance becomes saturated when the training data size is beyond 200.ConclusionManual de-identification of large-scale data is an impractical procedure since it is time-consuming and subject to human errors. Analysis of the NER model's performance in this study sheds light on a semi-automatic clinical de-identification pipeline for enterprise-wide data warehousing.
Experimental Investigation on Potential Effect of Cell Shape and Size on the Residual Stress in Solid Oxide Fuel Cells
Authors: Azari Khaled , Abdoli Hamid , Torabi Morteza , Bozorgmehri Shahriar 
Published: February 2022

In the manufacturing process of solid oxide fuel cells (SOFCs), the residual stresses and curvature are developed in components due to the differences in material properties of cell layers. Residual stress may lead to the crack formation in the cell layers and facilitates cell fracture. In this work, the changes of the residual stress in the electrolyte layer of the anode-supported planar solid oxide fuel cells are experimentally determined at room temperature. The “sin2ψ” technique of X-ray diffraction method is employed to measure the residual stress in the half-cell samples. Investigation on the changes of the residual stress and curvature state in the scaling-up process of the cell is crucial for commercial use. Therefore, several cells with different sizes and shapes are investigated to evaluate the potential impact of cell size and cell shape on the residual thermal stress. Values of about −610 MPa are determined for the electrolyte layer on an oxidized ∼400 μm thick anode substrate. The results reveal that despite the effect of size and shape on the radius of curvature, these parameters have no significant impact on the residual stress level.
Etiology and Clinical Features of Diplopia in South China: Analysis of 303 Cases
Authors: Wang Zhonghao , Zhu Binbin , Fu Licheng , Yan Jianhua 
Published: February 2022

PurposeTo provide a new classification system for diplopia and evaluate the etiology and clinical features of diplopia subtypes in south China.MethodsIn this retrospective study, all patients presenting with diplopia over the period from 2012 to 2014 in south China were reviewed. Patients were categorized into 3 groups according to their extraocular muscle (EOM) dysfunction: single EOM (sEOM), multiple EOMs (mEOMs), and a comitant strabismus group. Clinical data evaluated included age, sex, medical history, etiology and duration of diplopia, ocular alignment, and ocular motility.ResultsA total of 303 patients were enrolled. The most common type of EOM dysfunction was sEOM (158 cases, 52.1%), followed by mEOMs (n = 119, 39.3%), and finally the comitant strabismus group (n = 26, 8.6%). Overall, the most common cause of diplopia involved orbital diseases. Within the sEOM group, microangiopathy (n = 42, 26.6%) and trauma (n = 41, 25.9%) were the major etiologies, with the lateral rectus (LR) (n = 86, 54.4%) being the most frequently involved. There were 12 (4.0%) patients who were considered as nasopharyngeal carcinoma (NPC)-associated diplopia (10 caused by radiation neuropathy following radiation therapy). Thyroid associated ophthalmopathy (TAO, 56 cases, 47.1%) was the predominant etiology found in the mEOMs group. Acute acquired comitant esotropia (AACE, 14 cases, 53.9%) was the most common etiology in the comitant strabismus group.ConclusionsThis new classification system for assessing diplopia as based on EOM dysfunction represents an easy-to-follow approach that can be readily adapted for the clinical use. While microangiopathy and trauma represent common etiologies of diplopia, both orbital diseases and NPC-associated diplopia also warrant special attention when assessing diplopia within patients in south China.