bluuebunny/crossref_metadata_2025_split huggingface dataset:
A conserved sequence inDrosophilaArgonaute1 mRNA contributes to stress response via inducing miR-999 degradation
Authors: Sheng Peike , Li Lu , Li Tianqi , Wang Yuzhi , Hiers Nicholas M. , Mejia Jennifer S. , Sanchez Jossie S. , Zhou Lei , Xie Mingyi 
Published: November 2022

AbstractMicroRNAs (miRNAs) load onto Argonaute (AGO) proteins and target messenger RNAs (mRNA) to directly suppress gene expression at the post-transcriptional level. However, miRNA degradation can be triggered when there is extended base-pairing between miRNAs and the target RNAs. Such base-pairing can induce confirmational change of AGO and recruitment of ZSWIM8 ubiquitin ligase to mark AGO for proteasomal degradation, while the miRNAs are subsequently degraded. This target RNA-directed miRNA degradation (TDMD) mechanism appears to be an evolutionary conserved mechanism, but recent studies have focused on identifying endogenous TDMD events in the mammalian systems. Here, we performed AGO1-CLASH (crosslinking and sequencing of miRNA-mRNA hybrids) inDrosophilaS2 cells, withDora(ortholog of vertebrate ZSWIM8) knockout (KO) mediated by CRISPR-Cas9 to identify five TDMD triggers (sequences that can induce miRNA degradation). Interestingly, one trigger residing in the 3′ UTR ofAGO1mRNA induces miR-999 degradation. CRISPR-Cas9 KO of theAGO1trigger in S2 cells and inDrosophilaelevates miR-999 abundance, with concurrent repression of the miR-999 targets.AGO1trigger-KO flies respond poorly to hydrogen peroxide-induced stress, demonstrating the physiological importance of a single TDMD event.
Graphene-based thin film microelectrode technology forin vivohigh resolution neural recording and stimulation
Authors: Viana Damià , Walston Steven T. , Illa Xavier , Valle Jaume del , Hayward Andrew , Dodd Abbie , Loret Thomas , Prats-Alfonso Elisabet , de la Oliva Natàlia , Palma Marie , Corro Elena del , Rodríguez-Meana Bruno , Pilar Bernicola María del , Rodríguez-Lucas Elisa , Gener Thomas A. , de la Cruz Jose Manuel , Torres-Miranda Miguel , Duvan Fikret Taygun , Ria Nicola , Sperling Justin , Martí-Sánchez Sara , Spadaro Maria Chiara , Hébert Clément , Masvidal-Codina Eduard , Savage Sinead , Arbiol Jordi , Guimerà-Brunet Anton , Puig M. Victoria , Navarro Xavier , Yvert Blaise , Kostarelos Kostas , Garrido Jose A. 
Published: November 2022

AbstractNeuroprosthetic technology aims to restore nervous system functionality in cases of severe damage or degeneration by recording and stimulating the electrical activity of the neural tissue. One of the key factors determining the quality of the neuroprostheses is the electrode material used to establish electrical communication with the neural tissue, which is subject to strict electrical, electrochemical, and mechanical specifications as well as biological and microfabrication compatibility requirements. This work presents a nanoporous graphene-based thin film technology and its engineering to form flexible neural implants. Bench measurements show that the developed microelectrodes offer low impedance and high charge injection capacity throughout millions of pulses. In vivo electrode performance was assessed in rodents both from brain surface and intracortically showing high-fidelity recording performance, while stimulation performance was assessed with an intrafascicular implant that demonstrated low current thresholds and high selectivity for activating subsets of axons within the sciatic nerve. Furthermore, the tissue biocompatibility of the devices was validated by chronic epicortical and intraneural implantation. Overall, this works describes a novel graphene-based thin film microelectrode technology and demonstrates its potential for high-precision neural interfacing in both recording and stimulation applications.
Contribution of the collective excitations to the coupled proton and energy transport along mitochondrial crista membrane in oxidative phosphorylation system
Authors: Nesterov Semen V. , Yaguzhinsky Lev S. , Vasilov Raif G. , Kadantsev Vasiliy N. , Goltsov Alexey N. 
Published: November 2022

AbstractThe results of many experimental and theoretical works indicate that after transport of protons across the mitochondrial inner membrane (MIM) in oxidative phosphorylation system (OXPHOS), they are retained on the membrane-water interface in non-equilibrium state with free energy excess due to low proton surface-to-bulk release. This well-established phenomenon suggests that proton trapping on the membrane interface ensures vectorial lateral transport of protons from proton pumps to ATP synthases (proton acceptors). Despite the key role of the proton transport in bioenergetics, the molecular mechanism of proton transfer in the OXPHOS system is not yet completely established. Here, we developed a dynamics model of long-range transport of energized protons along the MIM accompanied by collective excitation of localized wave proragating on the membrane surface. Our model is based on the new data on the macromolecular organization of OXPHOS showing the well-ordered structure of respirasomes and ATP synthases on the cristae membrane folds. We developed a two-component dynamics model of the proton transport considering two coupled subsystems: the ordered hydrogen bond (HB) chain of water molecules and lipid headgroups of MIM. We analytically obtained two-component soliton solution in this model, which describes the motion of the proton kink, corresponding to successive proton hops in the HB chain, and coherent motion of a compression soliton in the chain of lipid headgroups. The local deformation in a soliton range facilitates proton jumps due to water molecules approaching each other in the HB chain. We suggested that the proton-conducting structures formed along the cristae membrane surface promote direct lateral proton transfer in the OXPHOS system. Collective excitations at the water-membrane interface in a form of two-component soliton ensure the coupled non-dissipative transport of charge carriers and elastic energy of MIM deformation to ATP synthases that may be utilized in ATP synthesis providing maximal efficiency in mitochondrial bioenergetics.
A dynamic atlas of immunocyte migration from the gut
Authors: Galván-Peña Silvia , Zhu Yangyang , Hanna Bola S. , Mathis Diane , Benoist Christophe 
Published: November 2022

SUMMARYDysbiosis in the gut microbiota impacts several systemic diseases. One possible mechanism is the migration of perturbed intestinal immunocytes to extra-intestinal tissues. Combining the Kaede photoconvertible mouse model and single-cell genomics, we generated a detailed map of migratory trajectories from the colon, at baseline and during intestinal and extra-intestinal inflammation. All colonic lineages emigrated from the colon in an S1P-dependent manner, dominated by B lymphocytes with a large continuous circulation of follicular B cells, which carried a gut-imprinted transcriptomic signature. T cell emigration was more selective, with distinct groups of RORγ+or IEL-like CD160+cells in the spleen. Gut inflammation curtailed emigration, except for DCs disseminating to lymph nodes. Colon emigrating cells distributed differentially to tumor, skin inflammation, or arthritic synovium, the former dominated by myeloid cells in a chemokine-dependent manner. These results thus reveal specific cellular trails originating in the gut, influenced by microbiota, which can shape peripheral immunity.
Phase transition of tensin-1 during the focal adhesion disassembly and cell division
Authors: Lee Yuh-Ru Julie , Yamada Soichiro , Lo Su Hao 
Published: November 2022

AbstractBiomolecular condensates are non-membranous structures that are mainly formed through liquid-liquid phase separation. Tensins are focal adhesion (FA) proteins linking the actin cytoskeleton to integrin receptors. Here we report that GFP-tagged tensin-1 (TNS1) proteins at physiological levels phase separate to form biomolecular condensates in TNS1 knockout cells. Live cell imaging showed that new TNS1 condensates are budding from the disassembling ends of FAs, and presence of these condensates is cell cycle dependent. TNS1 condensates dissolve immediately prior to mitosis and rapidly reappear while post-mitotic daughter cells establish new FAs. TNS1 condensates contain selected FA proteins and signaling molecules such as pT308Akt but not pS473Akt, suggesting previously unknown roles of TNS1 condensates in disassembling FAs, as the storage of core FA components and the signaling intermediates.
Genome-wide libraries for protozoan pathogens drug target screening using yeast surface display
Authors: Heslop Rhiannon , Gao Mengjin , Lira Andressa Brito , Sternlieb Tamara , Loock Mira , Sanghi Sahil Rao , Cestari Igor 
Published: November 2022

AbstractThe lack of genetic tools to manipulate protozoan pathogens has limited the use of genome-wide approaches to identify drug or vaccine targets and understand these organisms’ biology. We have developed an efficient method to construct genome-wide libraries for yeast surface display (YSD) and developed a YSD fitness screen (YSD-FS) to identify drug targets. We show the robustness of our method by generating genome-wide libraries forTrypanosoma brucei, Trypanosoma cruzi, andGiardia lambliaparasites. Each library has a diversity of ∼105to 106clones, representing ∼6 to 30-fold of the parasite’s genome. Nanopore sequencing confirmed the libraries’ genome coverage with multiple clones for each parasite gene. Western blot and imaging analysis confirmed surface expression of theG. lamblialibrary proteins in yeast. Using the YSD-FS assay, we identified bonafide interactors of metronidazole, a drug used to treat protozoan and bacterial infections. We also found enrichment in nucleotide-binding domain sequences associated with yeast increased fitness to metronidazole, indicating that this drug might target multiple enzymes containing nucleotide-binding domains. The libraries are valuable biological resources for discovering drug or vaccine targets, ligand receptors, protein-protein interactions, and pathogen-host interactions. The library assembly approach can be applied to other organisms or expression systems, and the YSD-FS assay might help identify new drug targets in protozoan pathogens.
Context-induced renewal of passive but not active coping behaviours in the shock-probe defensive burying task
Authors: Brown Alexa , Martins Melissa , Richard Isabelle , Chaudhri Nadia 
Published: November 2022

AbstractRenewal is the return of extinguished responding after removal from the extinction context. Renewal has been extensively studied using classical aversive conditioning procedures that measure a passive freezing response to an aversive conditioned stimulus. However, coping responses to aversive stimuli are complex and can be reflected in passive and active behaviours. Using the shock-probe defensive burying task, we investigated whether different coping responses are susceptible to renewal. During conditioning, male, Long-Evans rats were placed into a specific context (context A) where an electrified shock-probe delivered a 3 mA shock upon contact. During extinction, the shock-probe was unarmed in either the same (context A) or a different context (context B). Renewal of conditioned responses was assessed in the conditioning context (ABA) or in a novel context (ABC or AAB). Renewal of passive coping responses, indicated by an increased latency and a decreased duration of shock-probe contacts, was observed in all groups. However, renewal of passive coping, measured by increased time spent on the side of the chamber opposite the shock-probe, was only found in the ABA group. Renewal of active coping responses linked to defensive burying was not observed in any group. The present findings highlight the presence of multiple psychological processes underlying even basic forms of aversive conditioning and demonstrate the importance of assessing a broader set of behaviours to tease apart these different underlying mechanisms. The current findings suggest that passive coping responses may be more reliable indicators for assessing renewal than active coping behaviours associated with defensive burying.
Board governance and audit report lag in the light of big data adoption: the case of Egypt
Authors: Ahmed Hussein Mohsen Saber , El-Halaby Sherif , Albitar Khaldoon 
Published: November 2022

Purpose
This paper aims to examine the mediating role of big data adoption (BDA) on the association between board governance (BG) and audit report lag (ARL).

Design/methodology/approach
This study uses data extracted from financial reports for a sample from EGX100 over the period from 2015 to 2019. This study applies content analysis approach to measure the level of BDA. This study uses ordinary least squares, structure equation modelling and principal component analysis to investigate the relationship between BG, BDA and ARL.

Findings
The findings indicate that BDA can be used as a predictor of ARL for companies listed on the Egyptian stock exchange. The results show that board diversity has a significant effect on ARL when BDA is used as a mediator.

Research limitations/implications
This study only includes technology, telecommunications and health-care industries in the sample.

Practical implications
This paper raises investor and stakeholder awareness for the importance of BDA and corporate governance (CG) procedures in reducing audit report delays in developing countries such as Egypt. This study can assist regulators in developing audit report requirements and enforcing regulations to guarantee timely audit report publication.

Originality/value
This paper provides a shred of unique evidence on the role of BDA in mediating the relationship between BG and ARL in a developing country.
A new acoustically insulating fiber-reinforced lightweight concrete based on local materials and date palm waste
Authors: Boucedra Aissa , Bederina Madani 
Published: November 2022

Purpose
This paper aims to characterize and develop a new ecological lightweight concrete reinforced by addition of palm plant fibers (from vegetal waste) to be used in the thermal and acoustical insulation of local constructions. The date palm plant fibers are characterized by their low sensitivity to chemical reactions, low cost and large availability in local regions. Therefore, the newly obtained lightweight concrete may suggest a great interest, as it seems to be able to achieve good solutions for local construction problems, technically, economically and ecologically.

Design/methodology/approach
The experimental program focused on developing the composition of palm-fiber-reinforced concrete, by studying the effect of the length of the fibers (10, 20, 30 and 40 mm) and their mass percentage (0.5%, 1%, 1.5% and 2%), on the mechanical and acoustical properties of the composite. The main measured parameters were the compressive strength and flexural strength, sound absorption coefficient, noise reduction coefficient (NRC), etc. These tests were also borne out by the measure of density and water absorption, as well as microstructure analyses. To fully appreciate the behavior of the material, visualizations under optical microscope and scanning electron microscope analyses were carried out.

Findings
The addition of plant fibers to concrete made it possible to formulate a new lightweight concrete having interesting properties. The addition of date palm fibers significantly decreased the density of the concrete and consequently reduced its mechanical strength, particularly in compression. Acceptable compressive strength values were possible, according to the fibers content, while better values have been obtained in flexion. On the other hand, good acoustical performances were obtained: a considerable increase in the sound absorption coefficient and the NRC was recorded, according to the content and length of fibers. Even the rheological behavior has been improved with the addition of fibers, but with short fibers only.

Originality/value
Over the recent decades, many studies have attempted to search for more sustainable and environmentally friendly building materials. Therefore, this work aims to study the possibility of using waste from date palm trees as fibers in concrete instead of the conventionally used fibers. Although many researches have already been conducted on the effect of palm plant fibers on the mechanical/physical properties of concrete, no information is available neither on the formulation of this type of concrete nor on its acoustical properties. Indeed, due to the scarcity of raw materials and the excessive consumption of energy, the trend of plant fibers as resources, which are natural and renewable, is very attractive. It is therefore a major recycling project of waste and recovery of local materials.
It depends on personal networks: Feelings of stigmatisation among populist radical right party members
Authors: AMMASSARI SOFIA 
Published: November 2022

AbstractStigmatisation has been recognised as a major factor influencing the fortunes of populist radical right (PRR) parties. While scholars have examined it by taking parties as units of analysis, this study focuses on the individual level by asking Which PRR party members are more likely to feel stigmatised? After offering a novel theoretical explanation for feelings of stigmatisation based on the personal networks in which PRR grassroots members are embedded, it then investigates stigma using an original membership survey of about 7,000 members of the Sweden Democrats (SD) and interviews with 30 of them. The survey results show that the higher the educational qualification PRR grassroots members have achieved, the more likely they will feel stigmatised. In addition, those who have never had any relatives and/or friends in the SD, and those who are employed in the public sector, are more likely to consider membership discrediting. The interview data shed light on the survey results, by illustrating how public employees and university students find it hard to be open about their membership as they are surrounded by people with left‐wing views. By contrast, having relatives and/or friends who are members of the party reduces the stigma of joining PRR parties, becoming active in them, and talking about politics in public. Along with countering some of the prevailing wisdom about stigmatisation in PRR parties, the findings contribute to our understanding of PRR grassroots membership, which has long been an overlooked topic in the literature.
Comparison mitochondrial DNA DAMPs changes in platelet prepared by apheresis and PRP
Authors: Chegini Azita , Vakili Leila , Samiee Shahram , Shaiegan Mojgan 
Published: November 2022

AbstractIntroductionMt DNA (DAMPs) plays a key modulatory role in immune cells and may also mediate a variety of adverse transfusion reactions.Material and MethodThe cross‐sectional study was performed on 22 (PRP) and 14 (SDP) between February 2019 and 2020. mtDNA DAMPs by quantitative real‐time PCR was assessed on days 1, 3, and 5 of platelets storage. The data was entered in REST 2009 software, and the amount of fold change was calculated. Multiple t tests were also used.ResultsThe mtDNA DAMPs fold change in SDP on days 1–3, 1.3 times, on days 3–5, 1.5 times, and on days 1–5, 2.1 times increased. The fold change on days 1–3, 0.8 times, on days 3–5, 0.6 times, and on days 1–5, 0.49 times decreased in PRP products.ConclusionThe method of preparation and processing can affect mtDNA DAMPs fold changes.
Impact of folic acid on regulatory B lymphocytes from patients with systemic lupus erythematosus in vitro
Authors: Nikolova‐Ganeva Kalina , Vasilev Vasil , Kerezieva Simona , Tchorbanov Andrey 
Published: November 2022

AbstractBackgroundEpigenetic modifications of genomes are of particular interest as numerous studies indicate the correlation between DNA methylation and the development of systemic lupus. As a major methyl group donor, folic acid is an important participant in this process. The aim of this study is to determine the effect of low or high dose folate co‐culturing with peripheral blood mononuclear cells (PBMCs) on the secretion of interleukin (IL)10 from regulatory cells from lupus patients or from healthy volunteers.MethodsPBMCs from lupus patients and healthy volunteers were isolated and separated CD19+ B cell populations were cultured in the presence of 4 μg/mL or of 16 μg/mL of folic acid and the DNA methylation level as well as the percentages of B lymphocytes were measured. In another experiment, PBMCs were stimulated in vitro for IL10 production with 1 μg/mL recombinant human CD40L and with 2.5 μg/mL unmethylated CpG dinucleotides and cultured in the presence of 4 μg/mL or of 16 μg/mL of folic acid.ResultsAlthough co‐culturing with low or high folic acid concentrations had no effect on the methylation level of B lymphocytes, particular patients showed an increase in the population of CD19+ IL10+ as well as of CD19– IL10+ cells.ConclusionThe observed increase may be a consequence of additional indirect or direct methylation of DNA in specific loci of the targeted cells. However, further analysis would clarify the exact mechanism of action of folate and would reveal its immunomodulating potential in this autoimmune disease.
Moderate prenatal alcohol exposure produces sex‐specific social impairments and attenuates prelimbic excitability and amygdala‐cortex modulation of adult social behaviour
Authors: Przybysz Kathryn R. , Spodnick Mary B. , Johnson Julia M. , Varlinskaya Elena I. , Diaz Marvin R. 
Published: November 2022

AbstractLifelong social impairments are common in individuals with prenatal alcohol exposure (PAE), and preclinical studies have identified gestational day (G)12 as a vulnerable timepoint for producing social deficits following binge‐level PAE. While moderate (m)PAE also produces social impairments, the long‐term neuroadaptations underlying them are poorly understood. Activity of the projection from the basolateral amygdala to the prelimbic cortex (BLA → PL) leads to social avoidance, and the PL is implicated in negative social behaviours, making each of these potential candidates for the neuroadaptations underlying mPAE‐induced social impairments. To examine this, we first established that G12 mPAE produced sex‐specific social impairments lasting into adulthood in Sprague–Dawley rats. We then chemogenetically inhibited the BLA → PL using clozapine N‐oxide (CNO) during adult social testing. This revealed that CNO reduced social investigation in control males but had no effect on mPAE males or females of either exposure, indicating that mPAE attenuated the role of this projection in regulating male social behaviour and highlighting one potential mechanism by which mPAE affects male social behaviour more severely. Using whole‐cell electrophysiology, we also examined mPAE‐induced changes to PL pyramidal cell physiology and determined that mPAE reduced cell excitability, likely due to increased suppression by inhibitory interneurons. Overall, this work identified two mPAE‐induced neuroadaptations that last into adulthood and that may underlie the sex‐specific vulnerability to mPAE‐induced social impairments. Future research is necessary to expand upon how these circuits modulate both normal and pathological social behaviours and to identify sex‐specific mechanisms, leading to differential vulnerability in males and females.