bluuebunny/crossref_metadata_2025_split huggingface dataset:
The genome of the blind bee louse fly reveals deep convergences with its social host and illuminates Drosophila origins
Authors: Bastide Héloïse , Legout Hélène , Dogbo Noé , Ogereau David , Prediger Carolina , Carcaud Julie , Filée Jonathan , Garnery Lionel , Gilbert Clément , Marion-Poll Frédéric , Requier Fabrice , Sandoz Jean-Christophe , Yassin Amir 
Published: November 2022

SummaryThe nests of social insects often harbor a rich fauna of intruders known as inquilines.1Social inquilines are usually closely-related to their host due to potential genetic predispositions,2,3but how phylogenetically distant non-social inquilines adapt to their hosts remains unclear. Here, we analyzed the genome of the wingless and blind bee louse flyBraula coeca, an inquiline kleptoparasite of the Western honey beeApis mellifera.4,5Using large phylogenomic data, we confirmed recent accounts that the bee louse fly is an aberrant drosophilid,6,7and showed that it had likely evolved from a sap-breeder ancestor associated with honeydew and scale insects wax. Unlike many parasites, such as the human louse, the bee louse fly genome did not show significant erosion or strict reliance on an endosymbiont, likely due to a relatively recent age of inquilinism. However, a striking parallel evolution in a set of gene families was observed between the honey bee and the bee louse fly. Convergences included genes potentially involved in metabolism and immunity and the loss of nearly all bitter-tasting gustatory receptors in agreement with life in a protective nest and a major diet of honey, pollen, and beeswax. Vision-related and odorant receptor genes also exhibited rapid losses. Only genes whose orthologs in the closely relatedDrosophila melanogasterrespond to honey bee pheromones components or floral aroma were retained, whereas the losses included orthologous receptors responsive to the anti-ovarian honey bee queen pheromones. These results indicate that deep genomic convergences can underlie major morphological and neuroethological transitions during the evolution of inquilinism between non-social parasites and their social hosts.
Microglial process dynamics enhance neuronal activity by shielding GABAergic synaptic inputs
Authors: Haruwaka Koichiro , Ying Yanlu , Liang Yue , Umpierre Anthony D. , Yi Min-Hee , Kremen Vaclav , Chen Tingjun , Xie Tao , Dong Hailong , Worrell Gregory A. , Wu Long-Jun 
Published: November 2022

AbstractMicroglia are resident immune cells of the central nervous system (CNS) and play key roles in brain homeostasis. During anesthesia, microglia increase their dynamic process surveillance and interact more closely with neurons. However, the functional significance of microglial process dynamics and neuronal interaction has remained unclear. Usingin vivotwo-photon imaging in awake mice, we discover that microglia enhance neuronal activity after the cessation of general anesthesia. Hyperactive neuron somata are directly contacted by microglial processes, which specifically co-localize with GABAergic boutons. Electron microscopy-based synaptic reconstruction after two-photon imaging reveals that microglial processes enter into the synaptic cleft to shield GABAergic inputs. Microglial ablation or loss of microglial β2-adrenergic receptors prevent post-anesthesia neuronal hyperactivity. Together, our study demonstrates a previously unappreciated function of microglial process dynamics, which allow microglia to transiently boost neuronal activity by physically shielding inhibitory inputs.
Signature morpho-electric properties of diverse GABAergic interneurons in the human neocortex
Authors: Lee Brian , Dalley Rachel , Miller Jeremy A , Chartrand Thomas , Close Jennie , Mann Rusty , Mukora Alice , Ng Lindsay , Alfiler Lauren , Baker Katherine , Bertagnolli Darren , Brouner Krissy , Casper Tamara , Csajbok Eva , Dee Nick , Donadio Nicholas , Driessens Stan L.W. , Egdorf Tom , Enstrom Rachel , Galakhova Anna A , Gary Amanda , Gelfand Emily , Goldy Jeff , Hadley Kristen , Heistek Tim S. , Hill Dijon , Johansen Nelson , Jorstad Nik , Kim Lisa , Kocsis Agnes Katalin , Kruse Lauren , Kunst Michael , Leon Gabriela , Long Brian , Mallory Matthew , Maxwell Michelle , McGraw Medea , McMillen Delissa , Melief Erica J , Molnar Gabor , Mortrud Marty T , Newman Dakota , Nyhus Julie , Opitz-Araya Ximena , Pham Trangthanh , Pom Alice , Potekhina Lydia , Rajanbabu Ram , Ruiz Augustin , Sunkin Susan M , Szots Ildiko , Taskin Naz , Thyagarajan Bargavi , Tieu Michael , Trinh Jessica , Vargas Sara , Vumbaco David , Waleboer Femke , Weed Natalie , Williams Grace , Wilson Julia , Yao Shenqin , Zhou Thomas , Barzo Pal , Bakken Trygve , Cobbs Charles , Ellenbogen Richard G. , Esposito Luke , Ferreira Manuel , Gouwens Nathan W , Grannan Benjamin , Gwinn Ryder P. , Hauptman Jason S. , Hodge Rebecca , Jarsky Tim , Keene C.Dirk , Ko Andrew L. , Levi Boaz , Ojemann Jeffrey G. , Patel Anoop , Ruzevick Jacob , Silbergeld Daniel L. , Smith Kim , Waters Jack , Zeng Hongkui , Berg Jim , Goriounova Natalia A. , Kalmbach Brian , de Kock Christiaan P.J. , Mansvelder Huib D , Sorensen Staci A , Tamas Gabor , Lein Ed S. , Ting Jonathan T 
Published: November 2022

AbstractHuman cortical interneurons have been challenging to study due to high diversity and lack of mature brain tissue platforms and genetic targeting tools. We employed rapid GABAergic neuron viral labeling plus unbiased Patch-seq sampling in brain slices to define the signature morpho-electric properties of GABAergic neurons in the human neocortex. Viral targeting greatly facilitated sampling of the SST subclass, including primate specialized double bouquet cells which mapped to two SST transcriptomic types. Multimodal analysis uncovered an SST neuron type with properties inconsistent with original subclass assignment; we instead propose reclassification into PVALB subclass. Our findings provide novel insights about functional properties of human cortical GABAergic neuron subclasses and types and highlight the essential role of multimodal annotation for refinement of emerging transcriptomic cell type taxonomies.One Sentence SummaryViral genetic labeling of GABAergic neurons in humanex vivobrain slices paired with Patch-seq recording yields an in-depth functional annotation of human cortical interneuron subclasses and types and highlights the essential role of multimodal functional annotation for refinement of emerging transcriptomic cell type taxonomies.
Limitations of Solar-Induced Chlorophyll Fluorescence (SIF) for Estimating Photosynthesis Under Stress
Authors: Mayo Amir M. , Moshelion Menachem , Liran Oded 
Published: November 2022

AbstractHigh-throughput measurements of photosynthesis of plants grown under various conditions may provide important insights into the plasticity of the photosynthetic performance of plants. Remote sensing of photosynthetic activity is the next generation of fast scanning techniques, enabling high-throughput photosynthesis measurements under controlled conditions. We hypothesized that by measuring SIF simultaneously with whole-plant water relations in a standardized controlled drought experiment, we would be able to quantify photosynthetic activity and to detect water stress at an early stage. A functional-phenotyping platform was used to apply the controlled drought treatment and to monitor the growth and water balance of tomato introgression lines (ILs). A new SIF-derived index, electron transport rate (RS-ETRi), was found to be negatively correlated with whole-plant stomatal conductance (Gsc) under non-stressed conditions. No significant relationships were found between SIF and plant biomass or Gsc. SIF687responded to drought earlier than any of the other measured vegetation indices. SIF based indices could not differentiate between introgressed lines of tomato; whereas differences between Introgression Lines were clearly identified by the water-relations measurements. We concluded that SIF did not provide any advantage over commonly used methods for detecting physiological differences between the Introgression Lines. Overall, although SIF plays a significant role in photosynthesis, the relationship between SIF and photosynthesis is complex and we believe it would be an oversimplification to use SIF to quantify photosynthetic activity on close canopy spatial resolution level.
Impact of variants of concern on SARS-CoV-2 viral dynamics in non-human primates
Authors: Marc Aurélien , Marlin Romain , Donati Flora , Prague Mélanie , Kerioui Marion , Hérate Cécile , Alexandre Marie , Dereuddre-bosquet Nathalie , Bertrand Julie , Contreras Vanessa , Behillil Sylvie , Maisonnasse Pauline , Van Der Werf Sylvie , Grand Roger Le , Guedj Jérémie 
Published: November 2022

AbstractThe impact of variants of concern (VoC) on SARS-CoV-2 viral dynamics remains poorly understood and essentially relies on observational studies subject to various sorts of biases. In contrast, experimental models of infection constitute a powerful model to perform controlled comparisons of the viral dynamics observed with VoC and better quantify how VoC escape from the immune response.Here we used molecular and infectious viral load of 78 cynomolgus macaques to characterize in detail the effects of VoC on viral dynamics. We first developed a mathematical model that recapitulate the observed dynamics, and we found that the best model describing the data assumed a rapid antigen-dependent stimulation of the immune response leading to a rapid reduction of viral infectivity. When compared with the historical variant, all VoC except beta were associated with an escape from this immune response, and this effect was particularly sensitive for delta and omicron variant (p<10−6for both). Interestingly, delta variant was associated with a 1.8-fold increased viral production rate (p=0.046), while conversely omicron variant was associated with a 14-fold reduction in viral production rate (p<10−6). During a natural infection, our models predict that delta variant is associated with a higher peak viral RNA than omicron variant (7.6 log10copies/mL 95% CI 6.8 – 8 for delta; 5.6 log10copies/mL 95% CI 4.8 – 6.3 for omicron) while having similar peak infectious titers (3.7 log10PFU/mL 95% CI 2.4 – 4.6 for delta; 2.8 log10PFU/mL 95% CI 1.9 – 3.8 for omicron). These results provide a detailed picture of the effects of VoC on total and infectious viral load and may help understand some differences observed in the patterns of viral transmission of these viruses.
SARS-CoV-2 ORF8 limits expression levels of Spike antigen
Authors: Kim Ik-Jung , Lee Yong-ho , Khalid Mir M. , Zhang Yini , Ott Melanie , Verdin Eric 
Published: November 2022

SummarySurvival from COVID-19 depends on the ability of the host to effectively neutralize virions and infected cells, a process largely driven by antibody-mediated immunity. However, with the newly emerging variants that evade Spike-targeting antibodies, re-infections and breakthrough infections are increasingly common. A full characterization of SARS-CoV-2 mechanisms counteracting antibody-mediated immunity is needed. Here, we report that ORF8 is a SARS-CoV-2 factor that controls cellular Spike antigen levels. ORF8 limits the availability of mature Spike by inhibiting host protein synthesis and retaining Spike at the endoplasmic reticulum, reducing cell-surface Spike levels and recognition by anti-SARS-CoV-2 antibodies. With limited Spike availability, ORF8 restricts Spike incorporation during viral assembly, reducing Spike levels in virions. Cell entry of these virions leaves fewer Spike molecules at the cell surface, limiting antibody recognition of infected cells. Our studies propose an ORF8-dependent SARS-CoV-2 strategy that allows immune evasion of infected cells for extended viral production.
Persulfidation of DJ-1 : Mechanism and Consequences
Authors: Galardon Erwan , Mathas Nicolas , Padovani Dominique , Le Corre Laurent , Poncet Gabrielle , Dairou Julien 
Published: November 2022

AbstractDJ-1 (also called PARK7) is a ubiquitously expressed protein involved in the etiology of Parkinson disease and cancers. At least one of its three cysteine residue is functionally essential, and its oxidation state determines the specific function of the enzyme. DJ-1 was recently reported to be persulfidated in mammalian cell lines, but the implications of this post-translational modification have not yet been analyzed. Here, we report that recombinant DJ-1 is reversibly persulfidated at cysteine 106 by reaction with various sulfane donors and subsequently inhibited. Strikingly, this reaction is orders of magnitude faster than C106 oxidation by H2O2, and persulfidated DJ-1 behaves differently than sulfinylated DJ-1. Both these PTM most likely play a dedicated role in DJ-1 signaling or protective pathways.
Bending properties in the 4-halobenzonitrile crystals and C–halogen...N[triple-bond]C halogen bonds
Authors: Veluthaparambath Ragima V. P. , Krishna G Vibha , Pancharatna Pattath D. , Saha Binoy K. 
Published: November 2022

The single crystal of 4-iodobenzonitrile (C7H4IN) is brittle, whereas those of 4-bromobenzonitrile (C7H4BrN) and one of the two forms of 4-chlorobenzonitrile (C7H4ClN) are compliant in nature. The chloro crystal exhibits elastic bending, but in spite of having stronger halogen bonds, the bromo crystal exhibits plastic bending. Crystal structures have been analyzed to understand the different bending properties of these three crystals. In all three cases, the molecules form C—X...N[triple-bond]C (X = halogen) halogen-bonded chains in their respective crystal structures. Statistical analyses and DFT calculations on the C—X...N[triple-bond]C halogen bonds reveal that the optimum geometry of all three halogen bonds is linear and the C—I...N[triple-bond]C bond is strongest among the three. However, when the geometry deviates from linearity, the energy loss is very high in the case of the C—I...N[triple-bond]C bond compared to the other two systems. This explains why 4-iodobenzonitrile is brittle, whereas the other two are flexible. The interactions in 4-bromobenzonitrile are more isotropic than those in 4-chlorobenzonitrile. The iodo and chloro compounds crystallize in centrosymmetric space groups, whereas the crystal of the bromo compound lacks inversion symmetry. In spite of this difference in their space groups, the chloro and bromo crystals have very similar crystal packing. In the case of the bromo crystal, the halogen-bonded chains are parallel to the bending axis (long axis) of the crystal. However, these chains are significantly tilted in the case of the chloro crystal. The isotropic/anisotropic interactions, presence/absence of an inversion centre and the different alignment of the halogen-bonded chains with respect to the bending axis could explain the different bending properties of the chloro and bromo crystals.
Advance in grey system theory and applications in science and engineering
Authors: Liu Sifeng , Tao Yong , Xie Naiming , Tao Liangyan , Hu Mingli 
Published: November 2022

PurposeThe purpose of this paper is to summarize the advances in grey system theory research and various application achievements in science and engineering. At the same time, it commemorates the 40th anniversary of the birth of grey system theory and the 10th anniversary of Grey Systems–Theory and Application.Design/methodology/approachFirstly, the innovations of theoretical research in grey system theory were summarized and some of the widely recognized new results are briefly described. By searching and combing the research results of grey system theory in China national knowledge infrastructure (CNKI) database and Web of Science by Institute for Scientific Information (ISI), this paper shows the rapid development trend of grey system theory in the past 40 years, and the successful applications of grey system theory in the fields of social sciences, natural sciences and engineering technologies.FindingsMore than 227 thousands literature were found by input 10 phrases such as grey system, grey number and sequence operator etc. in CNKI database. After entering the new century, the number of grey system papers included in CNKI database is increasing rapidly. Since 2008, more than 10 thousands papers have been included per year and more than 15 thousands papers have been included per year since 2014. Grey system method and model are widely used in physics, chemistry, biology and other fields of natural science, as well as transportation, electric power, machinery and other fields of engineering technology, and a large number of valuable results have been achieved.Practical implicationsIt can be seen that the grey system theory plays an important role in promoting China’s scientific and technological progress, innovation and development and high-level talent training from tens of thousands of literatures marked with important national science and technology projects and a large number of grey system literatures published by China’s double first-class universities and double first-class discipline construction universities.Originality/valueBoth innovations of theoretical research and practical application play important role in the growth of new theory. The innovations of theoretical research provide methods and tools for practical application, which is conducive to improve application efficiency and broaden application fields. A large number of practical applications needs have become the source of theoretical innovation and the solid background for the birth of theoretical innovation achievements.
Examining the functionality of digital platform capability in driving B2B firm performance: evidence from emerging market
Authors: Liu Longjun , Long Jing , Fan Qing , Wan Wenhai , Liu Ruhong 
Published: November 2022

Purpose
This study aims to explore the influence mechanism of digital platform capability on firm performance in the business-to-business (B2B) context. This study draws on the core competence theory and the resource-based view and includes resource identification, resource allocation and intrapreneurship into the research framework.

Design/methodology/approach
Data were collected from 167 B2B firms with survey questionnaires in central, eastern and southeastern coastal areas of China. The firms were mainly involved in e-commerce, manufacturing, service industry and internet technology. Participants were mainly middle and senior managers with a comprehensive grasp of their firms’ information.

Findings
This study found that digital platform capability has a positive impact on a B2B firm’s performance. Resource identification, resource allocation and intrapreneurship play a chain mediating role between digital platform capability and firm performance. That is, digital platform capability could promote employee intrapreneurship through resource identification and resource allocation, thereby improving firm performance.

Practical implications
Aiming to gain performance, firms should pay attention to the construction of digital platforms, increase venture capital investment and provide more resources to support intrapreneurship.

Originality/value
Based on empirical evidence, to the best of authors’ knowledge, this is the first attempt to link digital platform capability and firm performance in the B2B context of emerging markets, providing a new perspective to clarify its relationship mechanism.
Understanding and conceptualising the daily work of human resource practitioners
Authors: Wallo Andreas , Coetzer Alan 
Published: November 2022

PurposeThis study aims to explore how human resource (HR) practitioners conceive of their practice, reveal challenges they grapple with in daily work and generate a conceptual framework of HR praxis.Design/methodology/approachThe paper is based on interviews with HR practitioners in Sweden and a review of articles that examine aspects of HR practitioners' work.FindingsThe HR practitioners' work is fragmented and reactive, filled with meetings and affords few opportunities to work undisturbed. Operational tasks are prioritised over strategic work, and their work sometimes involves tasks that are not HR's responsibility. The nature of HR practitioners' daily work mimics the work of their main “customer”, i.e. managers within the organisations.Practical implicationsThe HR practitioners were working mainly in the service of managers, which suggests that they have an internal focus. Consistent with current, prescriptive HR discourse, HR practitioners should adopt a multi-stakeholder perspective of human resource management (HRM) and a more external focus that is necessary to contribute to wider, organisational effectiveness. The findings could enrich what is taught in higher education by providing students with an account of the reality of HR practitioners' daily work.Originality/valueThe study provides a situated account of the daily work of HR practitioners, which is largely absent from the literature.
Driving a difference: the role of the Covid-19 pandemic in U.S. consumers’ information search behaviors and purchasing of Japanese automobiles
Authors: Namin Aidin , Ketron Seth 
Published: November 2022

Purpose
While prior research has investigated factors that predict consumers’ information search behaviors as they relate to automobiles, such studies were conducted prior to the COVID-19 pandemic. Given that the pandemic has necessitated lockdowns, social distancing, business closures and other disruptions to normal shopping activities, consumer information search behaviors have also been substantially altered as the psychological distance between consumers and marketers has increased. Thus, this study aims to examine these changes and identify patterns of search behavior for a major durable product: automobiles.

Design/methodology/approach
Using survey data from before and during the pandemic, the study implements Finite Mixture Modeling to unveil latent segments of U.S. consumers’ search behaviors and choices for Japanese automobiles. This analytic method enables capturing consumer unobserved heterogeneity through mixing probabilities guided by individual characteristics. These segments are determined based on consumers’ information search for online and offline marketer-controlled and nonmarketer-controlled sources.

Findings
The study identifies that two segments of consumers emerge both prior to the pandemic and during the pandemic. These empirically validated findings indicate that the pandemic has led to shifts in consumers’ information search behaviors for Japanese automobiles by relying more on nonmarketer-controlled sources of information.

Originality/value
This work is among the first comprehensive empirical analyses of consumer search for a major durable product by comparing pre- and during pandemic patterns. Using analytics and econometrics, the first-hand analysis findings offer meaningful implications for marketers and product managers in the automotive industry.