bluuebunny/crossref_metadata_2025_split huggingface dataset:
Suxiao Jiuxin Pills, a Chinese herbal complex, block human potassium channel current hKv1.5 in CHO cells
Authors: Zhang Minzhou , Yu Juan , Qu Jiashin , Qi Jianyong 
Published: May 2022

Abstract
Kv1.5 channel plays key roles in cardiac action potential and atrial repolarization. Suxiao Jiuxin Pills (SXJ), an herbal remedy had been widely used in China in treating angina pectoris and atrial arrhythmia. However, whether SXJ regulates Kv1.5 channels is still unclear. The goal of the present study was to examine the effects of SXJ on cloned human potassium channels (hKv1.5) in Chinese hamster ovary (CHO) cells. The ionic and gating currents were detected; dose-dependent curve, current-voltage curve, steady-state activation curve (SSA), activation time constant (τact), deactivation (τdeact), and gating currents were compared between SXJ and control. Further, intracellular application of SXJ was implemented. Results showed that SXJ inhibited the hKv1.5 current in a time-, dose- and voltage-dependent manner with a half maximal inhibitive concentration (IC50) value of 38.85 milligram per liter. The voltage dependence was described by an equivalent electrical distance δ of 0.15, which suggested that at the binding site SXJ experienced 15% of the applied transmembrane electrical field. There were no significant different changes of SSA, τact, τdeact , ‘On’ gating current (on-Ig), and ‘off’ gating current (off-Ig) between SXJ and Control (P > 0.05). Intracellular application of SXJ could block the hKv1.5 current significantly (P < 0.05). In conclusion, SXJ produces a dose-, time-, and voltage-dependent block of hKv1.5, with interacting electrical distance δ of 0.15. SXJ did not influence the gating currents and deactivation time course. SXJ was an open-blocker, which provided the experimental research of SXJ in clinical treating atrial arrhythmia.
Integrated multi-omics reveals the beneficial roles of chlorogenic acid in improving the immune function of immunologically-stressed broilers
Authors: Liu Huawei , Li Xuemin , Zhang Kai , Lv Xiaoguo , Zhang Quanwei , Chen Peng , Wang Yang , Zhao Jinshan 
Published: May 2022

Abstract
Background:
Commercial broilers under intensive production are highly vulnerable to various stressors, especially immunologically. It has been reported that chlorogenic acid (CGA) could affect the intestinal microbiota and barrier function and elicit several health benefits, including improved immune function. Complex interrelations regulate the dynamic interplay among gut microbiota, host, and diverse health outcomes. The aim of the present study was to elucidate the immunoregulatory mechanisms of CGA using multi-omics approaches.
Results:
CGA supplementation significantly improved jejunal morphology, jejunal barrier function, and immune function in dexamethasone (DEX)-treated broilers. Moreover, in DEX-CGA-treated broilers, the increase in gut microbiome diversity was consistent with change in taxonomic composition, especially in the Clostridiales_vadinBB60_group. Additionally, the levels of short chain fatty acids increased remarkably after CGA supplementation, which was consistent with the results of Kyoto Encyclopedia of Genes and Genomes pathway analysis for different gut microbes. Moreover, through multiple reaction monitoring, significant enrichment was observed in the pyruvate fermentation and butanonate metabolism pathways in the DEX-CGA group. Proteomic and metabolomic analyses revealed that CGA treatment increased the expression of several health promoting proteins (TMSB4X and LGMN) and metabolites (2,4-dihydroxy benzoic acid and homogentisic acid), and regulated the peroxisome proliferator-activated receptor (PPAR) and mitogen-activated protein kinase (MAPK) pathways, which were supported by western blotting results. Pearson’s correlation analyses showed significant correlations between altered parameters of immune function, jejunal barrier function, and different microbiota, proteins, and metabolites.
Conclusions:
Overall, our data indicated that CGA treatment improved the performance and immunological functions of DEX-treated broilers by regulating gut microbiota, especially Clostridiales_vadinBB60_group, and PPAR and MAPK pathways. The result offer novel insights into CGA-mediated improvement in immune function and intestinal health.
Klotho alleviate contrast-induced acute kidney injury by suppressing oxidative stress, inflammation and NF-kappaB/NLRP3-mediated pyroptosis
Authors: Fu Yanbin , Wei Xuebiao , Cao Jianfeng , Ge Yanzhi , Su Zedazhong , Yu Danqing 
Published: May 2022

Abstract

Background
Contrast-induced acute kidney injury (CI-AKI) is a common complication following percutaneous coronary intervention in coronary artery disease (CAD) patients with more than 30% incidence. Klotho is a multifunctional protein that inhibits oxidative stress and inflammation, but its role in CI-AKI is poorly understood. The present study aimed to explore the effects of klohto in CI-AKI.
Methods
Six-week-old mice and HK-2 were divided into control group, contrast medium (CM) group, CM + klotho group, and klotho group. Kidney injury was evaluated by HE staining. And the renal function was shown by Scr and BUN. Cell viability and damage were determined by CCK-8 assay and lactate dehydrogenase (LDH) activity assay. Oxidative stress related indicators including intracellular reactive oxygen species (ROS), superoxidase dismutase (SOD), and malondialdehyde (MDA) were tested by fluorescent probe dichloro-dihydro-fluorescein diacetate (DCFH-DA) and enzyme-linked immunosorbent assay (ELISA) assay. IL-6, TNF-α, IL-1β, and IL-18 in the cell supernatant were tested by ELISA assay and used to reflect inflammation responses. Propidium iodide (PI) staining showed the cell death of HK-2. The expression of NF-κB, phosphorylated NF-κB (p-NF-κB), and pyroptosis-related protein levels of NLRP3, caspase-1, GSDMD, and cleaved-GSDMD were detected by western blot.
Results
Exogenous Klotho administration reduced kidney histopathological alterations and improved renal function in vivo. In vitro, Klotho significantly inhibited CM-induced oxidative stress and productions of both IL-6 and TNF-α. Moreover, we found klotho inhibited the activation of p-NF-κB and down-regulated pyroptosis-related protein (NLRP3, caspase-1, GSDMD, and cleaved-GSDMD).
Conclusion
The findings suggested that klotho had a protective effect on CI-AKI via suppressing oxidative stress, inflammation and NF-κB/NLRP3-mediated pyroptosis, contributing to the potential therapy of CI-AKI.
Sleep and the Nigerian Environment: Understanding the True Markers of Sleep Quality
Authors: Ismaila Ismaila S.Olasukanmi , Odumosu Odumosu O.Odugbemi , Kuye Kuye S.I. , Folorunso Folorunso Olusegun 
Published: May 2022

Abstract
Sound sleep aids sound health and good ageing, unfortunately, people still wake up in the morning feeling tired, sleep at work, and sometimes unnecessarily aggressive. It is normal to experience sleeplessness once in a while, but it is not normal to regularly have problems getting to sleep at night and to wake up feeling exhausted. Poverty, socioeconomic instability, inadequate medicare etc. have continued to hamper the quality of sleep for the majority of the populace. Sleep helps the brain to function properly and not getting enough sleep or poor quality sleep has many consequences: behavioural problems in children, hypertension, fatigue, cardiovascular diseases, mental health problems etc. in adults. This study evaluated subjective sleep qualities (timing of sleep, environmental factors, health status, and socioeconomic status) on the sleep status of an average Nigerian adult in selected areas of South-West Nigeria. A descriptive analysis was used for the study as contained in the SPSS version 15.0 software on a total population of 207 adults. A self-developed questionnaire validated by experts in the field producing an Alpha reliability co-efficient of 0.735 was used for the study analysis. Findings from the study revealed no significant relationship between gender, religious affiliations and sleep quality, whereas, age, nature of work, health status, body height, marriage, the timing of sleep and hobbies have critical negative and positive significances with sleep qualities respectively. In all, the subjective sleep factors as indicated in the study could be depended upon in rating the sleep quality of an average Nigerian adult.
Prevalence of frailty and cognitive impairment in older transplant candidates. A preview to the Kidney Transplantation in Older People (KTOP): impact of frailty on outcomes study
Authors: Thind Amarpreet Kaur , Rule Annabel , Goodall Dawn , Levy Shuli , Brice Sarah , Dor Frank JMF , Evans Nicola , Ospalla David , Thomas Nicola , Wellsted David , Johannsson Lina , Willicombe Michelle , Brown Edwina A 
Published: May 2022

Abstract
BackgroundKidney transplantation in older people has increased, however older transplant recipients experience mixed outcomes that invariably impacts on their quality of life. The increased vulnerability of older end stage kidney disease patients to frailty and cognitive impairment may partially explain the differences in outcomes observed. The Kidney Transplantation in Older People (KTOP): impact of frailty on clinical outcomes study is an active clinical study aiming to explore the experience of older people waiting for and undergoing transplantation. In this manuscript we present the study protocol, the study cohort, and the prevalence of frailty and cognitive impairment identified at recruitment. MethodsThe KTOP study is a single centre, prospective, mixed methods, observational study. Recruitment began in October 2019. All patients aged 60 or above either active on the deceased donor waitlist or undergoing live donor transplantation were eligible for recruitment. Recruited participants completed a series of questionnaires assessing frailty, cognition, and quality of life, which are repeated at defined time points whilst on the waitlist and post-transplant. Clinical data was concurrently collected. Any participants identified as frail or vulnerable to frailty were also eligible for enrolment into the qualitative sub-study. Results208 participants have been recruited (age 60-78). Baseline Montreal Cognitive Assessments were available for 172 participants, with 72 (41.9%) participants identified as having scores below normal (score <26). Edmonton Frail Scale assessments were available for 184 participants, with 30 participants (16.3%) identified as frail (score ³8), and a further 37 participants (20.1%) identified as being vulnerable to frailty (score 6-7). ConclusionIn the KTOP study cohort we have identified a prevalence of 41.9% of participants with MoCA scores suggestive of cognitive impairment, and a prevalence of frailty of 16.3% at recruitment. A further 20.1% were vulnerable to frailty. As formal testing for cognition and frailty is not routinely incorporated into the work up of older people across many units, the presence and significance of these conditions is likely not known. Ultimately the KTOP study will report on how these parameters evolve over time and following a transplant, and describe their impact on quality of life and clinical outcomes.
Long-term prognostic implications of residual left ventricular hypertrophy and systolic dysfunction in aortic stenosis following transcatheter aortic valve replacement
Authors: Pedersen Anders Lehmann Dahl , Povlsen Jonas Agerlund , Rasmussen Vibeke Guldbrand , Frederiksen Christian Alcaraz , Christiansen Evald Høj , Terkelsen Christian Juhl , Vase Henrik , Poulsen Steen Hvitfeldt 
Published: May 2022

Abstract
Purpose: The impact of left ventricle (LV) hypertrophy (LVH) regression on contractility, the extent of residual cardiac dysfunction and prognostic implications after the initial remodeling process after transcatheter aortic valve replacement (TAVR) has not been investigated. We aimed to assess whether greater LV mass regression from pre-TAVR to 12-months after TAVR was associated with increased contractility and systolic function; and assess the long-term prognostic value of residual LVH, systolic function and contractility 12-months after TAVR.Methods: A total of 439 symptomatic patients were included and examined by echocardiography. LVH regression was assessed as percentage change in LV mass index (LVMi) from baseline to 12-months after TAVR. Midwall fractional shortening (mFS) and stress-corrected (SC-mFS) were used to assess contractility. Primary outcome was all-cause mortality.Results: SC-mFS increased from 0.94 (0.7) at baseline (BS) to 1.22 (0.7) (p<0.05) 12-months after TAVR for patients with the most LVH regression, compared to patients with no LV regression (BS 1.06 (0.7) to 1.04 (0.5), NS).At 12-months after TAVR, multivariate analysis showed independent prognostic value of LVEF<50% or GLS<15% (HR 1.59, p=0.049) and mFS <14% (HR 1.99, p=0.002) for future all cause death.Conclusions: LVH regression in AS after TAVR is associated with significant improvements of LV contractility in contrast to patients without LV regression. Residual LVH and subsequent LV systolic dysfunction is substantial 12 months after TAVR and are associated with reduced survival. Impaired mFS and the combination of abnormal LVEF or GLS independently predicted all-cause mortality beyond 12 months after TAVR.
Combining Bioinformatics and Experiments to Identify MAD2L2 as a Key Regulator in Ovarian Cancer
Authors: Xu Kejun , Zheng Xiaojiao , Shi Hongyan , Ou Jilan , Ding Huiqing 
Published: May 2022

Abstract
Ovarian cancer (OVCA) is a common gynecological malignant tumor and the fourth most common female tumor, following breast cancer, cervical cancer and endometrial cancer. Spindle Assembly checkpoint (SAC) can finely control the dynamic balance between kinetosomes and microtubules during mitosis. MAD2L2 is a chromatin binding protein and selective shear body of MAD2, one part of SAC. However, the expression and prognostic values of MAD2L2 in OVCA have not been clarified. GEPIA, UALCAN, cBioPortal, GeneMANIA, DAVID 6.8, Metascape, TIMER, Kaplan-Meier and in vito cell experiments were utilized in this study. MAD2L2 was over expression in OVCA patients and accompanied with poor survival time, especially in grade four patients. MAD2L2’s mRNA alteration way was amplification and ubiquitination was the mainly regulation way. Significant GO term analysis showed that MAD2L2 were located mainly in the organellar ribosome, where they participate primarily in NADH dehydrogenase activity. They also acted as structural constituents in mitochondrial translation elongation. KEGG pathway analysis showed enrichment in the sulfur relay system and ribosome. Four types of immune module were used and the results showed that MAD2L was relate to CD4, CD8, B cell, NK cell and Myeloid dendritic cell. At last, high level of MAD2L2 promoted cell proliferation and migration of OVCA and upregulation of MAD2L2 suppressing ferroptosis process and accompanied by mTOR signaling in OVCA cells. Therefore, suppressing MAD2L2 might be a next choice of treatment of OVCA patients. In addition to the common strategies, like chemotherapy, preventing mTOR signaling might also be a better treatment method in the future.
Characterization of Cutaneous Melanoma: Carcinogenesis Beyond Ultraviolet Light
Authors: Loss Lairane Bridi , Santi Juliana Casotti , Lima Sarah Aparecida Fernandes , Lorencini Mariana Santos , Ferri Sabrina Chaves Costa , Junior Rogério Pimentel Terra , Chiarelli-Neto Orlando 
Published: May 2022

Abstract
Melanoma is the most aggressive type of skin cancer due to its metastatic potential, and its incidence has increased considerably in recent decades. Sun exposure to ultraviolet A and B rays remains the main risk factor for the development of this type of cancer, however, other factors involved are family history, artificial tanning, melanocytic nevi, low phototype and melanin photosensitization by visible light (VL). Through early diagnostic detection of the malignant lesion, it is possible to initiate therapy in an early and curative way, increasing survival with a subsequent decrease in the overall mortality rate. Advances in immunotherapy and targeted therapy have changed treatment paradigms and improved the prognosis of late-stage disease. Due to the high prevalence of the disease worldwide, the objective was to review the main aspects described in the literature regarding the etiology, pathophysiology, clinic, diagnosis, and management of the pathology, with special emphasis on cutaneous melanoma upon VL exposure.
Neutrophilia during hospitalization predicts ARDS and risk of death in COVID-19 patients
Authors: Ghazi Bouchra , Azhary Khadija El , Kantri Aziza , ElGhanmi Adil , Bakkouri Jalila El , Aidaoui Karim El , Haoudar Amal , Dini Nezha , Naitlho Abdelhamid , Kettani Chafik El , Chamlal Hasna , Nejjari Chakib , Badou Abdallah 
Published: May 2022

Abstract
Background
Coronavirus disease 2019 (COVID-19), poses a threat to the global public health. Early identification of critical cases is crucial to providing timely treatment to patients. Here, we investigated whether white immune cell levels could predict respiratory decline, complications and mortality.
Methods
We performed a retrospective study including 119 patients presenting with COVID-19. We divided our cohort into two categories, using two different classifications. Comparison tests, prediction analysis and the logistic regression analysis were used in this study.
Results
The study revealed that a rise in neutrophils levels was associated with respiratory deterioration and intubation. In contrast, neutrophils levels plateaued in patients who remained stable. Neutrophils levels, during the first 5 days of hospitalization, positively correlated with the procalcitonin and C-reactive protein levels. Interestingly, neutrophils levels at day 5 proved to be a better predictor of intubation and acute respiratory distress syndrome than initial neutrophils counts, C-reactive protein or procalcitonin. Neutrophils counts at day 5 higher than 7,7 (109/l) (normal range: 1,4–7,7 (109/L) also effectively predicted patient mortality, independently of usual clinical classifications. Moreover, binary logistic regression with stratified revealed that neutrophils at day5 > 7,7 (109/l) was an independent risk factor of mortality and acute respiratory distress syndrome. Finally, neutrophils levels at day 5 and the proinflammatory cytokine IL-6 were correlated, which suggested a mechanistic link.
Conclusions
Our data showed that neutrophils count at day 5, rather than at admission, proved to be an excellent predictor of complications and mortality during hospitalization and could be used to improve COVID-19 patients’management.
Micellar Enhanced Spectrofluorimetric Determination of Diphenylpyraline HCl and Dual Detection Cyclodextrin Micellar Liquid Chromatographic Method for Simultaneous Determination of Diphenylpyraline HCl, Paracetamol and Caffeine in Dosage Form
Authors: Hammad Sherin F. , El-Malla Samah F. , El-Khateeb Basma Z. 
Published: May 2022

Abstract
Highly sensitive micellar spectrofluorimetric method (Method I) has been developed and validated for the determination of diphenylpyraline HCl in pharmaceutical tablets and in plasma. Sodium dodecyl sulfate at pH 5 enhances the fluorescence intensity of diphenylpyraline at 286 nm that allow its determination at nano-level in plasma with mean percent recovery ± S.D of 99.719 ± 0.338. In addition, Green cyclodextrin micellar liquid chromatographic method (Method II) has been developed and validated for simultaneous determination of diphenylpyraline, paracetamol and caffeine using cyclodextrin micellar mobile phase consisted of 30 mM Brij*35, 0.5 mM hydroxypropyl β-cyclodextrin and phosphate buffer pH 4: MeOH (95:5, %v/v) that allows their simultaneous determination with enhanced spectrofluorimetric detection of diphenylpyraline. Method II was successfully applied for the simultaneous determination of diphenylpyraline, paracetamol and caffeine in a ternary laboratory prepared mixture containing all possible excipients with mean percent recoveries ± S.D of 100.176 ± 1.008, 101.166 ± 0.415 and 100.708 ± 1.836, respectively. The methods are verified to have excellent greenness.
Automatic comprehensive radiological reports for clinical acute stroke MRIs
Authors: Liu Chin-Fu , Zhao Yi , Miller Michael , Hillis Argye , Faria Andreia 
Published: May 2022

Abstract
Background: Although artificial intelligence systems that diagnosis among different conditions from medical images are long term aims, specific goals for automation of human-labor, time-consuming tasks are not only feasible but equally important. Acute conditions that require quantitative metrics, such as acute ischemic strokes, can greatly benefit by the consistency, objectiveness, and accessibility of automated radiological reports.Methods: We used 1,878 annotated clinical brain MRIs from patients with acute ischemic strokes to generate a fully automated artificial intelligence based system that outputs radiological reports in addition to the infarct volume, 3D digital infarct mask, and the feature vector of anatomical regions affected by the acute infarct. Findings: The performance of our system was comparable to that of an expert evaluator. The weight of the components of the feature vectors that supported the prediction of the reports, as well as the prediction probabilities are outputted, making the pre-trained models behind our system interpretable. The system is publicly available, runs in real time, in local computers, with minimal computational requirements, and it is readily useful for non-expert users.Interpretation: Our system supports large-scale processing of new and legacy data, enabling clinical and translational research. The generation of reports indicates that our fully automated system is able to extract quantitative, objective, structured, and personalized information of potential clinical relevance from stroke MRIs.
Effects of SARS-CoV-2 infections in patients with cancer on mortality, ICU admission and incidence: A systematic review with meta-analysis involving 709.908 participants and 31.732 cancer patients
Authors: Arayici Mehmet Emin , Kipcak Nazlican , Kayacik Ufuktan , Kelbat Cansu , Keskin Deniz , Kilicarslan Muhammed Emin , Kilinc Ahmet Veli , Kirgoz Sumeyye , Kirilmaz Anil , Kizilkaya Melih Alihan , Kizmaz Irem Gaye , Kocak Enes Berkin , Kochan Enver , Kocpinar Begum , Kordon Fatmanur , Kurt Batuhan , Ellidokuz Hulya 
Published: May 2022

Abstract
Background
Cancer patients constitute one of the highest-risk patient groups during the COVID-19 pandemic. In this study, it was aimed to perform a systematic review and meta-analysis to determine both the incidence and ICU (Intensive Care Unit) admission rates and mortality in SARS-CoV-2 infected cancer patients.
Methods
The PRISMA guidelines were closely followed during the design, analysis, and reporting of this systematic review and meta-analysis. A comprehensive literature search was performed for the published papers in PubMed / Medline, Scopus, medRxiv, Embase, and Web of Science (WoS) databases. SARS-CoV-2 infection pooled incidence in the cancer populations and the risk ratio (RR) of ICU admission rates / mortality in cancer and non-cancer groups, with 95% confidence intervals (CIs), were calculated using the random-effects model.
Results
A total of 58 studies, involving 709.908 participants and 31.732 cancer patients, were included in this study. The incidence in cancer patients was calculated as 8% (95% CI: 8% − 9%). Analysis results showed that mortality and ICU admission rate was significantly higher in patients with cancer (RR = 2.26, 95% CI: 1.94– 2.62, P < 0.001; RR = 1.45, 95% CI: 1.28–1.64, p < 0.001, respectively).
Conclusion
As a result, cancer was an important comorbidity and risk factor for all SARS-CoV-2 infected patients. This infection could result in severe and even fatal events in cancer patients. Cancer is associated with a poor prognosis in the COVID-19 pandemic. Cancer patients should be assessed more sensitively in the COVID-19 outbreak.