bluuebunny/crossref_metadata_2025_split huggingface dataset:
The perceived role and effectiveness of patient feedback system in strengthening therapeutic relationships in rural Tanzania
Authors: Isangula Kahabi 
Published: November 2022

Abstract
Introduction:
Patient feedback system forms an important entry point for medical personnel and healthcare administrators to identify healthcare service delivery gaps and develop responsive interventions. This may foster patient trust consequently increasing healthcare-seeking, engagement in decision, continuity, and satisfaction. However, research on patient feedback systems in rural primary healthcare settings appears limited.
Objective
The paper builds on recent research on patient-provider relationships in rural Tanzania to examine the role and effectiveness of patient feedback systems in improving therapeutic relationships.
Methods
The paper examines the findings of qualitative descriptive research in rural Tanzania that employed a human-centred design approach to co-create an intervention package for improving nurse-client relationships between January and September 2022. The study used semi-structured interviews in Swahili with purposefully selected providers, patients, and administrators to explore drivers of poor provider-patient relationships in the Shinyanga region of rural Tanzania. Interviews were concurrently translated and transcribed, then systematically coded to facilitate the development of themes using a thematic analysis approach. Findings related to patient feedback systems are used for this paper.
Findings:
Most participants cited the benefits of an effective Patient feedback system as reducing patients’ confusion around the complaints process, promoting patients’ positivity towards providers and hospitals, and reducing tensions between patients and providers. Suggestion boxes were the most cited Patient feedback system however, there were widespread concerns about their accessibility and effectiveness. While the providers (nurses) and administrators described suggestion boxes as widely available, they affirmed not receiving feedback or complaints from patients for a very long time. Contrastingly, most patients described suggestion boxes as largely unavailable and ineffective, with concerns of non-user friendliness and non-responsiveness dominating.
Conclusion
Despite their potential benefits in healthcare service quality improvements, the availability, user-friendliness, and responsiveness of patient feedback systems continue to be challenging. A call is made to providers, health administrators and researchers to prioritise the patient feedback system as both a useful entry point to reducing tensions in therapeutic relationships and, a tool for improving patient service uptake, continuity with care and satisfaction.
The efficacy of prophylactic metoclopramide in preventing nausea and vomiting in patients with acute pain given tramadol: A randomized double-blinded placebo-controlled trial
Authors: Sriwi Haruethai , Lokeskrawee Thanin , Patumanond Jayanton , Phinyo Phichayut , Wongyikul Pakpoom 
Published: November 2022

Abstract
Objective
This study aimed to conduct a randomized placebo-controlled trials to examine the clinical efficacy of prophylactic metoclopramide in reducing the incidence of nausea and vomiting in ED patients with acute pain and were treated with intravenous tramadol.
Results
The study protocol was registered in Thai Clinical Trials Registry (TCTR) [TCTR20220525001] in 21 October 2021. The Institutional Review Board of Lampang Hospital approved the study protocol (CERT NO. 103/64). A total of 99 ED patients presented with acute pain were recruited. Sixty-four patients were randomized, 31 patients in the treatment arm and 33 in the control arm. Overall, there were no significant differences in baseline characteristics between treatment arm and control arm. Only one patient within each arms reported having nausea symptoms. No patients reported vomiting episode. There was no statistically significant difference in the proportion of patients with nausea or vomiting symptoms between the two groups (3.1% in the treatment arm vs. 3.2% in the control arm, p = 1.000). The administration of prophylactic metoclopramide may not provide additional benefit in reducing the occurrence of nausea and/or vomiting episode in ED patients with acute pain treated with intravenous tramadol.
Comprehensive Bioinformatics Analysis Identifies a Novel Cellular Senescence Associated Factor-Related Signature to Predict Prognosis and Efficacy of Immunotherapy in Lung Squamous Cell Carcinoma
Authors: Ren Wei-xing , Zhao Qi , Zhao Ji-dong , Cui Xing , Chen Xin 
Published: November 2022

Abstract
PURPOSE:Cellular senescence-related genes (SRS) plays a key role in tumorigenesis, progression, and immune regulation of cancer. We explored the role of SRS in the development of LUSC and how SRS regulates LUSC immunophenotype and predicted possible novel small molecule drugs.
METHODS: We systematically analysised the clinical information and RNA-seq data related to patients with LUSC.Novel molecular subpopulations were identified using consensus clustering.Determined the tumor immune status of identified subgroups.Constructed and validated the prognostic risk model and clinical nomogram.Differential analysis of tumor stemness,immune checkpoints,and ICB responses was performed by SRS risk-score.For drug-screening and molecular docking, CMAP, LINCS, and AutodockVina technology were used.
RESULTS: The survival rates of molecular subgroups differed significantly. The hyperimmune state was significant in the poorer prognosis subgroup. SRS affected the immune status of LUSC patients, and when combined with risk patterns and clinical characteristics, it could lead to a correct prediction of LUSC prognosis. High SRS risk-scores were associated with higher ICB responses, and immune checkpoint scores, and lower tumor stemness.Dasatinib may become a new targeted drug for LUSC.
CONCLUSIONS:Overall, we identified prognostic SRS features in patients with LUSC, which were closely related to TIME. Further, reliable risk prediction models and clinical nomograms were constructed.The differences of ICB,tumor stemness,and immune checkpoint scores responses among patients with different risk scores were significant.Dasatinib was selected as a new small molecule for the potential treatment of LUSC[1].
Evaluation method of visual design quality of enterprise brand packaging based on cloud computing
Authors: Yi Liu 
Published: November 2022

Abstract
At present, people's daily consumption is more and more extended to the level of personalized demand, and various brands of goods are everywhere. People rarely make detailed analysis and screening from the quality when choosing goods, and often buy according to the brand. Therefore, many enterprises begin to pay attention to the innovation of modern packaging design functions. On the basis of the previous packaging identification, product protection and corporate image beautification functions, they pay more attention to the creation of brand packaging image and the innovation of its communication means, so as to convey product information to consumers more clearly, make the brand's value characteristics consistent with consumers' needs, and thus be accepted and recognized by consumers. However, based on the clustering of modern packaging design elements and the homogenization of similar packaging products, most enterprises still use the traditional packaging design teaching philosophy, and the quality of corporate brand visual design is often lack of innovation, and there is no unified evaluation standard. The cultural connotation, combined with the market situation, calculates the market packaging direction and quality demand through cloud computing, so as to improve the design aesthetic quality and enhance the brand packaging design ability. The experiment proves that our method has positive significance for enterprises in meeting the requirements of enterprise brand strategy and humanization and personalization of commodity packaging design.
In Vitro Screening for Antitrypanosomal Potentials of Punica granatum L. Leaves Crude Extracts
Authors: Fathuddin Muhammad Muhsin , Inabo Helen Ileigo 
Published: November 2022

Abstract
Purpose: Punica granatum (P. granatum) L. leaves were examined for potential antitrypanosomal properties. These leaves were acquired and identified at Ahmadu Bello University (A.B.U.) Herbarium Unit in Zaria. Following drying, the following solvents—chloroform, ethyl acetate, and ethanol—were used for exhaustive Soxhlet extraction. The crude extracts phytochemical analysis and in vitro antitrypanosomal capability were performed on Trypanosoma brucei brucei (T. b. brucei).
Research Method: The National Research Institute for Chemical Technology (NARICT), Basawa, Zaria, obtained the organism T. b. brucei. The wet and thick blood film method and the rapid matching method were used to examine the antitrypanosomal activity under 400x magnification. In 96-round-bottom well microtiter plates, the in vitro trypanocidal activity was evaluated in duplicate.
Findings: The antitrypanosomal activity of the ethanol and ethyl acetate extracts ranged from 6.25 to 400 mg/ml. Red blood cells (RBCs) were destroyed at all concentrations between 200 and 400 mg/ml, while between 6.25 and 100 mg/ml, the RBCs were still intact. As concentrations dropped, the parasite's motility rose. The parasite's motility entirely stopped after 60 minutes, whereas it continued for an additional 80 minutes in the negative control. A standard medicine that was made per the manufacturer's instructions and used as the positive control cleared everything in less than a minute.
Original/Value: This research may help in the development of novel antitrypanosomal medications from P. granatum L. As a result, despite lysing the RBC and having no effect on the parasite, the chloroform extract did not kill it. At a minimum concentration of 6.25 mg/ml, the P. granatum L. ethyl acetate and ethanol extract have the potential to operate as antitrypanosomal agents.
PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras
Authors: Escobar Denis , Pérez Fernando , Ortiz Bryan , Fontecha Gustavo 
Published: November 2022

Abstract
Background
Vector populations are a key target for malaria control and elimination. In Honduras there are at least 12 reported anopheline species, however, the definitive number of species remains uncertain. Due to the inherent limitations of morphological identification of Anophelesspecies, molecular approaches have been developed to provide accurate identification and robust surveillance of local malaria vectors. The aim of this study was to design and assess three PCR-RFLP assays to identify anopheline species in Honduras.
Methods
Mosquitoes captured between 2018 and 2022 in seven malaria-endemic and non-endemic departments in Honduras were analysed. The ITS2 ribosomal region and three restriction enzyme-based assays were evaluated in silico and experimentally.
Results
A total of 132 sequences from 12 anopheline species were analysed. The ITS2 marker showed length polymorphisms that generated products between 388 bp and 592 bp and no relevant intraspecies polymorphisms were found. Furthermore, the three PCR-RFLP assays were able to differentiate eleven species with sufficient precision and resolution.
Conclusion
The ITS2 region showed to be a useful molecular marker for identifying local Anophelesspecies. In addition, the PCR-RFLP assays evaluated here proved to be capable of discriminating most of the anopheline species present in Honduras. These methods provide alternatives to improve entomological surveillance of Anophelesin Honduras and other Mesoamerican countries.
No evidence of sustained nonzoonotic Plasmodium knowlesi transmission: estimating species-specific spatiotemporally varying reproduction numbers for malaria in Malaysia
Authors: Fornace Kimberly , Topazian Hillary , Routledge Isobel , Asyraf Syafie , Jelip Jenarun , Lindblade Kimberly , Jeffree Mohammad Saffree , Cuenca Pablo Ruiz , Bhatt Samir , Ahmed Kamruddin , Ghani Azra C , Drakeley Chris 
Published: November 2022

Abstract
Reported incidence of the zoonotic malaria Plasmodium knowlesi has markedly increased across Southeast Asia and threatens malaria elimination. Nonzoonotic transmission of P. knowlesi has been experimentally demonstrated, but it remains unknown whether nonzoonotic transmission is contributing to increases in P. knowlesi cases. Here, we adapt model-based inference methods to estimate RC, individual case reproductive numbers, for P. knowlesi, P. falciparum and P. vivax human cases in Malaysia from 2012–2020 (n = 32,635). Best fitting models for P. knowlesi showed subcritical transmission (RC <1) consistent with a large reservoir of unobserved infection sources, indicating P. knowlesi remains a primarily zoonotic infection. In contrast, sustained transmission (RC > 1) was estimated historically for P. falciparum and P. vivax, with declines in RC estimates observed over time consistent with local elimination. Together, this suggests sustained nonzoonotic P. knowlesi transmission is highly unlikely and that new approaches are urgently needed to control spillover risks.
Comparison of Postoperative Analgesia Use Between Robotic and Laparoscopic Total Hysterectomy: A Retrospective Cohort Study
Authors: Tanabe Shohei , Yamamoto Ryohei , Sugino Sachiyo , Ichida Kotaro , Niiya Kiyoshi , Morishima Syuji 
Published: November 2022

Abstract
Background/Objective:Although robotic and laparoscopic total hysterectomies are widely used as minimally invasive procedures, consensus on which is superior regarding lesser postoperative pain is lacking. Thisstudy determines whether there is a difference in the proportion of postoperative use of non-steroidal anti-inflammatory drugs (NSAIDs) and acetaminophen between robotic and laparoscopic total hysterectomies.
Methods: This retrospective cohort study enrolled patients who underwent robotic or laparoscopic total hysterectomy for uterine fibroids, adenomyosis, or cervical intraepithelial neoplasia grade 3 at a hospital between July 2016and November 2021. The outcome was postoperative analgesics (i.e., NSAIDs or acetaminophen) use. Unadjusted and adjusted logistic regression analyses were performed to evaluate the association between the procedure and outcome. Adjusted variables were age, body mass index, surgeon’s laparoscopic technique certification, intravenous patient-controlled analgesia, and wound local anesthesia.
Results: Of 127 patients, 3 were excluded, and 124 were included. Robotic and laparoscopic hysterectomy was performed in 38 and 86 patients, respectively. Postoperative analgesics were administered to 10 (26.3%) and 52 (60.5%) patients in the robotic and laparoscopic groups, respectively. Unadjusted logistic regression analysis showed significantly more frequent analgesics use in the laparoscopy group (odds ratio [OR] 4.28; 95% confidence interval [CI] 1.85–9.93; p < 0.01). Adjusted logistic regression analysis did not detect significant differences (OR 2.62; 95% CI 0.91–7.56; p = 0.07).
Conclusion: No significant difference in the proportion of postoperative analgesia was observed betweenrobotic total hysterectomy and laparoscopy. Future studies must include largersample sizes andaligned intraoperative and postoperative analgesic management.
Platelet-rich plasma preserves cartilage thickness and delays total knee arthroplasty in osteoarthritis with an inflammatory phenotype: a 5-year follow-up retrospective study
Authors: Chen Yang , Wu Song , Wen Zi , Wang Xin-Xing , Chen Yong , Xia Guang , Cao Xu 
Published: November 2022

Abstract
Background
This study aims to explore whether platelet-rich plasma (PRP) can delay the progression of disease, reduce the incidence of Total knee arthroplasty (TKA) and improve clinical symptoms in patients with typical inflammatory phenotype knee osteoarthritis (KOA)
Methods
This was a retrospective cohort study with 5-year follow-up. According to clinical manifestations, magnetic resonance imaging (MRI) Osteoarthritis Knee Score (MOAKS), and serum inflammation markers C-reactive protein (CRP), we selected patients with typical inflammatory phenotype of KOA. Patients were divided into groups based on whether they had received PRP, hyaluronic acid (HA), or other conservative treatment (OCT). The Kellgren-Lawrence (K-L) grade and Minimum joint space width (MJSW) in knee X-rays were used to evaluate the progression of KOA. The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores, Knee Society scores (KSS), minimal clinically important difference (MCID) and Osteoarthritis Research Society International Set Responder Criteria Osteoarthritis Clinical Trials Revisited (OMERACT-OARSI) tool were used to evaluate the improvement of KOA symptoms. The incidence and timing of TKA was statistically analyzed.
Results
A total of 646 patients were finally included, including 211 received PRP, 209 received HA and 226 received OCT. PRP showed better results in K-L grade and MJSW compared with HA and OCT (The results at 12m, 24m, 36m, 48m, 60m, respectively, were as follows; K-L grade, PRP vs. HA, P = 0.957, P = 0.534, P = 0.230, P < 0.001, P < 0.001; PRP vs. OCT, P = 0.240, P = 0.012, P = 0.004, P < 0.001, P < 0.001; MJSW, PRP vs. HA, P = 0.249, P = 0.013, P < 0.001, P < 0.001, P < 0.001; PRP vs. OCT, P = 0.155, P = 0.001, P < 0.001, P < 0.001, P < 0.001). Compared with HA and OCT, PRP group exhibited significant lower TKA incidence (PRP vs. HA, P = 0.001; PRP vs. OCT, P = 0.001; HA vs OCT, P = 0.732) and delayed time to TKA (log-rank, PRP vs HA,P < 0.001, PRP vs OCT, P < 0.001, HA vs OCT, P = 0.467). The WOMAC, KSS and KSS-F in PRP group were significantly better than those in HA group and OCT group at each time point after treatment (P < 0.05).
Conclusions
Intra articular injection of PRP can delay progression of KOA, reduce or postpone occurrence of TKA and improve clinical symptoms in strictly screened patients with typical inflammatory phenotype KOA.
Level of Evidence:
III, retrospective cohort.
Investigation of scarce input data augmentation for modelling nitrogenous compounds in South African rivers
Authors: Mahlathi Christopher Dumisani , Wilms Josefine , Brink Isobel 
Published: November 2022

Abstract
In this study, basic interpolation and machine learning data augmentation were applied to scarce data used in Water Quality Analysis Simulation Programme (WASP) and Continuous Stirred Tank Reactor (CSTR) that were applied to nitrogenous compound degradation modelling in a river reach. Model outputs were assessed for statistically significant differences. Furthermore, artificial data gaps were introduced into the input data to study the limitations of each augmentation method. The Python Data Analysis Library (Pandas) was used to perform the deterministic interpolation. In addition, the effect of missing data at local maxima was investigated. The results showed little statistical difference between deterministic interpolation methods for data augmentation but larger differences when the input data were infilled specifically at locations where extrema occurred.
Methodology for an ecological solution of subsurface flow constructed wetlands used in the treatment of greywater
Authors: Anigrou Yassine , Bahlami Ahmed , El Khlifi Mohamed 
Published: November 2022

Abstract
Hammams or public baths continue to consume substantial quantities of drinking water up to 120 m3/day and discharge equivalent quantities of greywater. These hammams thus become an important source of this greywater, which can be easily treated using constructed wetlands (CWs). In this context, the present study proposes to practitioners a general method for sizing subsurface flow (SSF) CWs for the treatment of greywater discharged from hammams. It is oriented to simple applications such as irrigation, car washing and toilet flushing. Due to the complexity of quantifying the evapotranspiration (ET) of the treated water at the CWs, a practical and flexible method is presented here to calculate ET. In the end, a case study of a Moroccan hammam has been treated and discussed. It provides the designers of SSF CWs with a concrete example of the application of the proposed methodology.
Non-canonical Small GTPase RBJ Promotes NSCLC Progression Through the
Canonical MEK/ERK Signaling Pathway
Authors: Dong Zhe , Hu Weidong , Wang Yujin , Shen Xiaoyan , Wang Qingwen , Guo Zixin , Hu Liwen 
Published: November 2022

Background:
Although the majority of members belonging to the small GTPase Ras superfamily have been studied in several malignancies, the function of RBJ has remained unclear, particularly in non-small cell lung cancer (NSCLC).

Objective:
The research aims to determine the function of RBJ in NSCLC.

Methods:
The levels of RBJ protein in tumor tissue and para-carcinoma normal tissue were ascertained via immunohistochemistry (IHC). The growth, migration, and invasion of NSCLC cells were assessed by 5-ethynyl-2-deoxyuridine (EdU) assay, colony formation, cell counting kit-8 (CCK8), transwell and wound healing assays. Furthermore, a nude mouse xenograft model was established to study the function of RBJ in tumorigenesis in vivo.

Results:
The IHC analysis revealed that the protein levels of RBJ were notably increased in tumor tissue and positively associated with the clinical stage. In addition, the knockdown of RBJ restrained the growth, invasion, and migration of NSCLC cell lines by inhibiting the epithelial-mesenchymal transition (EMT) through the MEK/ERK signaling pathway. Accordingly, opposite results were observed when RBJ was overexpressed. In addition, the overexpression of RBJ accelerated tumor formation by A549 cells in nude mice.

Conclusion:
RBJ promoted cancer progression in NSCLC by activating EMT via the MEK/ERK signaling. Thus, RBJ could be used as a potential therapeutic against NSCLC.
Laccase-assisted Bioremediation of Pesticides: Scope and Challenges
Authors: Paul Chandana , Pal Nilasish , Maitra Madhumita , Das Nirmalendu 
Published: November 2022

Abstract:
Laccase (Benzenediol: oxygen oxidoreductase; E.C.1.10.3.2), a multicopper oxidase that is
a known lignin-degrading enzyme, can catalyse an ample array of substrates, from phenolic, nonphenolic
compounds, aromatic amines, diamines, heterocyclic compounds to organic/inorganic metal
compounds, etc., bestowed they have not too high redox potentials. Despite many laccase-producing
organisms like bacteria, insects, plants, and animals, white rot filamentous fungi are the best producers
of this enzyme. In the presence of laccase, pesticides (fungicides, herbicides, insecticides, etc.) of various
chemical compositions (organophosphates, organochlorines, carbamates, pyrethrin & pyrethroids,
etc.) are oxidized into the water with collateral reduction of four electrons of molecular oxygen with
various efficiencies. Bioremediation efficiency can be increased in the presence of various natural or
synthetic mediators, viz. ABTS, violuric acid, 1- hydroxy benzotriazole, vanillin, syringaldehyde, PEG,
etc. Immobilized laccase on various supporting materials increased the enzyme's stability, reliability,
and reusability for continuous application, particularly for industrial processes. The present review
discusses the structure, catalytic cycle, general mechanism of oxidation, and various scopes and challenges
of pesticide degradation by this multifaceted biocatalyst which could lead to a green sustainable
environment.