bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selective Single-Bacteria Extraction Based on Capture and Release of Microemulsion Droplets
Authors: Li Jiyu , Hu Dinglong , Lim Chee Kent , Ren Jifeng , Yao Xin , Ma Chao , . Marcos , Chen Weiqiang , Lee Patrick K. H. , Lam Raymond H. W. 
Published: January 2022

Abstract
Human host-associated microbial communities in body sites can reflect health status based on the population distribution and specific microbial properties in the heterogeneous community. Bacteria identification at the single-cell level provides a reliable biomarker and also pathological information for clinical diagnosis. Nevertheless, biosamples extracted from some body sites cannot offer sufficient sample volume and number of target cells as required by most of the existing single-cell isolation methods such as flow cytometry. Herein we report a novel integrated microfluidic system, which consists of a microemulsion module for single-bacteria encapsulation and a sequential microdroplet capture and release module for selectively extract only the single-bacteria encapsulating microdroplets. We optimize the system for a success rate of the single-cell extraction to be >80%. We further verify applicability of the system with prepared cell mixtures (Methylorubrum extorquens AM1 and Methylorubrum extorquens BG8) and biosamples collected from human skin, in order to quantify the population distribution of multiple key species in a heterogeneous microbial community. Results indicate perfect viability of the single-cell extracts and compatibility with downstream analyses such as PCR. Together, this research demonstrates that the reported single-bacteria extraction system can be applied in microbiome and pathology research and clinical diagnosis as a clinical or point-of-care device.
Mesenchymal Stem Cell therapy for COVID-19-induced ARDS patients. A successful phase1, randomized, control-placebo group, clinical trial
Authors: Farkhad Najmeh Kaffash , Sedaghat Alireza , Reihani Hamidreza , Moghadam Amir Adhami , Moghadam Ahmad Bagheri , Ghaebi Nayereh Khadem , Khodadoust Mohamad Ali , Ganjali Rashin , Tafreshian Amirreza , Afshari Jalil Tavakol 
Published: January 2022

Abstract
BackgroundAcute respiratory distress syndrome (ARDS) is the devasting complication of the new COVID-19 pandemic, directly correlated with releasing large amounts of inflammatory cytokines. Due to their immunoregulatory features, Mesenchymal Stem Cells (MSCs) provide a promising approach against this disease. In this regard, this study was designed as a single-center, open-label, phase 1 clinical trial with a control group to examine the safety and efficacy of three injections of Umbilical Cord-derived MSCs (UC-MSCs) in mild-moderate COVID-19-induced ARDS patients.MethodsTwenty confirmed COVID-19 patients with mild to moderate ARDS degree (using the WHO guideline for ARDS classification) entered the study and were randomly divided into two groups: control group (standard care) and intervention group (standard care + UC-MSCs). The patients received three intravenous infusions of UC-MSCs (1 × \({10}^{6}\) cells/kg BW per injection) every other day. Respiratory markers, CRP levels and some serum cytokines were assessed four times (days of 0,5,10 and 17) during the 17 days follow-up period.ResultsDuring the study, there were no serious adverse effects after cell transplantations. Besides, significant improvement in SPO2/FIO2 ratio and serum CRP levels was observed. On the other hand, a significant decrease (P<0.05) in serum cytokine levels of IL-6, IFN-g, TNF-α, IL-17 A and a significant increase in serum cytokine levels of TGF-B, IL-1B and IL-10 was observed. Also, no significant changes were observed in CT scan images of patients during the study period.ConclusionOur obtained results demonstrated that multiple intravenous transplantations of allogenic UC-MSCs in non-severe COVID-19-induced ARDS patients are a safe procedure. In addition, this intervention is a hopeful approach to decline cytokine storm and recover respiratory functions. Indeed, more clinical trials with larger sample sizes are required to confirm these results.Trial registrationThis clinical trial was registered with the Iranian Registry of Clinical Trials (ID: IRCT20160809029275N1 at 2020.05.30).
A Note On “Pythagorean Uncertain Linguistic Hesitant Fuzzy Weighted Averaging Operator and Its Application in Financial Group Decision Making”
Authors: Appadoo S.S. , Makhan Mohammadreza , Kumar Amit 
Published: January 2022

Abstract
Shakeel et al. (Soft Comput. 24 )2020(1585-1597) proposed the concept of a Pythagorean uncertain linguistic hesitant fuzzy set (PULHFS), some arithmetic operations of Pythagorean uncertain linguistic hesitant fuzzy sets (PULHFSs), an approach for comparing PULHFSs and a Pythagorean uncertain linguistic hesitant fuzzy weighted averaging (PULHFWA) operator as well as it extensions. Also, using the proposed comparing approach and the proposed aggregation operators, Shakeel et al. proposed a method for solving multi-attribute group decision making (MAGDM) problems. In future other researchers may use Shakeel et al.’s work in their research work. However, it is observed that that the approach for comparing PULHFSs and aggregation operators, proposed by Shakeel et al. (2020(, are not appropriate. Hence, the method for solving MAGDM problems, proposed by Shakeel et al., is also not appropriate. The aim of this note is to make the researchers aware about the inappropriateness of Shakeel et al.’s work. Furthermore, to point out that to resolve the inappropriateness of Shakeel et al.’s work (2020) is a challenging open research problem.
The effect of two easily-implementable modifications in reducing gender disparities in Door-to-Needle time in acute ischemic stroke
Authors: Jameie Meilka , Jameie Mana , Farahmand Ghasem , Ilkhani Saba , Magrouni Hana , Ranjbar Zahra , Heydari Sanaz , Shahbazi Mojtaba , Kaeedi Maryam , Amiri Rosita , Alizade Fateme , Balali Pargol , Amanollahi Mobina , Varedi Mahnaz , Pourghaz Bahareh , Ghabaee Mojdeh 
Published: January 2022

Abstract
Introduction: Door-to-needle (DTN) time is an important factor in managing stroke for which studies have reported delays in women. We aimed to evaluate the effect of some simple modifications on reducing sex disparities, with a focus on DTN. Methods: This longitudinal cohort study was conducted between September 2019, and August 2021, at a comprehensive stroke center. Previously we utilized the conventional “D’s of stoke care” for timely management. Some simple modifications were implemented in September 2020, with a special focus on the time of the neurologist's evaluation (D4-B) and patients’ transfer to the stroke unit (D6-B). Patients were analyzed in two groups: group 1, before, and group 2, after employing the modifications. Sex as the main variable of interest along with other selected covariates were regressed towards the DTN, using univariable and multivariable logistic regressions. Results: We enrolled 47 and 56 patients who received intravenous thrombolysis in groups 1 and 2, respectively. Although there was a significant difference in DTN≤ 1 hour between women and men in group 1 (36% vs. 52%, p= 0.019), it was not significantly different in group 2 (48% vs. 48.4%, p= 0.97). Furthermore, regression analysis showed being female was a significant predictor of DTN> 1 hour in group 1 (aOR= 6.65, p= 0.02), while after employing the modifications gender was not a predictor of delayed DTN. Conclusion: Although we have a long way to achieve performance measures in developed countries, we seem to have succeeded in reducing gender disparities in DTN using these simple modifications.
Effect of WHO SCC Use Supported with Yearlong On-Site Mentoring on Quality of Intrapartum Care in High Load Public Sector Hospitals in Andhra Pradesh, India: Pre-Post Mixed Methods Evaluation
Authors: Singh Samiksha , Kannuri Nanda Kishore , Mishra Aparajita , Gaikwad Leena , Shukla Rajan , Tyagi Mukta , Charmarthy Swechha 
Published: January 2022

Abstract
Background
Quality of intrapartum care is essential for improving pregnancy outcomes; several models for improving performance are tested, globally. Dakshata is one such WHO SCC-based national program—improving resources, providers’ competence, and accountability—in public sector secondary care hospitals of India. Andhra Pradesh state devised strategy of mentoring by the handpicked member from within the obstetric team, supported by external strategic partner. We evaluated the effectiveness and assessed contextual factors to success of the program.
Methods
We conducted pre and post mentoring mixed-method surveys to evaluate the change in evidence-based intrapartum and newborn care practices and stillbirth rates, across 23 of 38 eligible hospitals. We directly observed obstetric assessments and childbirth, extracted data from casesheets and registers, interviewed beneficiaries and conducted facility surveys. We in-depth interviewed stakeholders from state, district and facility managers, mentors and obstetric staff, and external managers for theory-driven qualitative assessment.
Results
After one year, average adherence to practices sustained high during admission (81%, 81%); improved during childbirth (78%, 86%; p=0.016); moderate within one hour of birth (72%, 71%), and poor postpartum care before discharge (46–43%). Stillbirths reduced from 11(95% CI, 9-13) to 4(3-5) per 1000 births (p<0.001). Some practices did not improve even after sustained reinforcement. Commitment from state, engaging district officers, monitoring and feedback by external managers enabled supportive setting. The structured training and mentoring package, and periodic assessments delivered under supervision ensured the standards of mentoring. The mentoring model is acceptable, effective, less costly and scalable; appears sustainable if state commits to institutionalising a long-term mentoring with adequate monitoring.
Conclusion
The SCC-based mentoring and skill building program showed improvement in practices during childbirth while it sustained high levels of care during admission, but no improvement in postpartum care. The state needs to monitor and ensure continuous mentoring with required infrastructural support.
Advantages of a Centrifugal Pump in the Development of a Swine Model of an Artificial Placenta
Authors: Charest-Pekeski Alex J. , Cho Steven K.S. , Aujla Tanroop , Sun Liqun , Floh Alejandro A. , McVey Mark J. , Sheta Ayman , Estrada Marvin , Crawford-Lean Lynn , Foreman Celeste , Mroczek Dariusz , Belik Jaques , Saini Brahmdeep S. , Lim Jessie Mei , Moir Olivia J. , Lee Fu-Tsuen , Quinn Megan , Darby Jack R.T. , Seed Mike , Morrison Janna L. , Haller Christoph 
Published: January 2022

Abstract
The recent demonstration of normal development of preterm sheep in an artificial extrauterine environment has renewed interest in artificial placenta (AP) systems as a potential treatment strategy for extremely preterm human infants. However, the feasibility of translating this technology to the human preterm infant remains unknown. Here we report the support of 13 preterm fetal pigs delivered at 102±4 days (d) gestation, weighing 616±139g with a circuit consisting of an oxygenator and a centrifugal pump, comparing these results with our previously reported pumpless circuit (n=12; 98±4d; 743±350g). The umbilical vessels were cannulated, and fetuses were supported for 46.4±46.8 hours using the pumped AP versus 11±13 hours on the pumpless AP circuit. Upon initiation of AP support on the pumped system, we observed supraphysiologic circuit flows, tachycardia, and hypertension, while animals maintained on a pumpless AP circuit exhibited subphysiologic flows. On the pumped AP circuit, there was a progressive decline in UV flow and oxygen delivery. We conclude that the addition of a centrifugal pump to the AP circuit improves survival of preterm pigs by augmenting UV flow through the reduction of right ventricular afterload. However, we continued to observe the development of heart failure within a matter of days.
Prognostic Analysis of PTEN Mutation in Endometrial Carcinoma
Authors: Ye Chaopeng , Ma Yuanqiao , Zhang Qi , li Guoqing , Zhao Wei 
Published: January 2022

Abstract
Purpose: Endometrial carcinoma (EC) is recognized as one of the most common female reproductive system malignant tumors. Great efforts have been made to improve the diagnosis and prognostic analysis of EC, however, more sensitive prognostic evaluation system for EC remains to be established. The mutation status of PTEN and its association with clinical characteristics, as well as its prognostic value in EC need to be evaluated.Methods: TCGA dataset comprised of 526 EC cases were used to perform a comprehensive analysis of PTEN mutation. Kaplan‐Meier survival plot were performed to analyze the clinical significance of PTEN mutation. GO, KEGG pathway and IPA network analysis were performed to identify the specific pathways for PTEN mutation patients. And the prognostic value of PTEN mutation was further analyzed.Results: Here, the highest mutation frequency of PTEN (65%) was found in EC from Pan cancer. PTEN mutation status was significantly correlated with the better prognosis of EC patients. A total of 66 differentially expressed genes (DEGs) were identified between PTEN-mutant and PTEN-wild groups. KEGG pathway enrichment analysis suggested that some key pathways were relevant to the tumorigenesis and development of EC. The DEGs were mainly associated with endocrine and reproduction related pathways based on the IPA analysis. Then, a PTEN related-genes prognostic signature including CLDN9, UCHL1, BEX2, SLC47A1, PGR, SLC25A35, SCGB2A1, MSX1, CRABP2 and MAL was established. The K-M result showed the significant differences of survival rate between high- and low- risk groups. Furthermore, univariate and multivariate Cox regression analysis results indicated that age, figo_stage and risk score could be identified as independent prognostic factors. Finally, a nomogram for predicting survival rate was established.Conclusion: The above data suggested that PTEN mutation status could be serviced as a potential prognostic biomarker for EC, which might guide future EC personalized treatment.
A Five-time-recurring Kawasaki Disease: A Case Report and Literature Review
Authors: chen fang , Wang Manzhi , Wei Songqing 
Published: January 2022

Abstract
BackgroundFive-time-recurring Kawasaki Disease without any cardiovascular complications is relatively rare and was first described in 2021.Case presentationWe describe a case of five-time-recurring Kawasaki disease (KD) in a 6-year-old boy who had been diagnosed with typical KD without cardiac involvement at age 25 months. Subsequently, the child was hospitalized with KD other 4 times when he was 40, 47, 59, and 66-month-old respetively. In each episode, the boy was characterized by an abrupt intermittently high fever but typical cutaneous and mucosal manifestations were not always presented. Clinical improvement was successfully obtained after intravenous immunoglobulin and (or) aspirin in each time without any cardiovascular damage. Diagnosis was difficult to establish because of the incomplete clinical features and the recurrence of the disease.ConclusionPhysicians need to be aware of the differential diagnosis of relapsing KD should be kept in mind while treating a patient with a history of KD. Five-time-recurring Kawasaki Disease is infrequent and will increase the likelihood of poor clinical outcomes if not diagnosed and managed promptly.
The Precision of Different Types of Plates Fabricated with a Computer-Aided Design and Manufacturing System in Mandibular Reconstruction with Fibular Free Flaps
Authors: Yodrabum Nutcha , Rudeejaroonrung Krit , Viriya Natthaphat , Chaikangwan Irin , Kongkunnavat Natthapong , Tianrungroj Jirapat , Ongsiriporn Mathee , Piyaman Parkpoom , Puncreobutr Chedtha 
Published: January 2022

Abstract
Background
Computer-assisted surgery (CAS) has been introduced to mandible reconstruction with fibular free flap in cutting guide placement. When CAS is cooperated with different plate fixations, the results show various degrees of errors by which this study aimed to evaluate.
Methods
Mock surgeries were conducted in 3D-printed mandibles with either 2 types of defects; limited or extensive, reconstructed from 2 ameloblastoma patients. Three types of fixations; miniplate, manually bending reconstruction plate and patient-specific plates are tested, each of which was performed 3 times in each type of defects, adding up to 18 surgeries. One with the least errors was selected and applied with patients whose 3D-printed mandibles derived. Finally, in vivo errors were compared with the mock.
Results
In limited defect, average errors show no statistical significance among all types. In extensive defect, patient-specific plate had a significantly lower average condylar error than manually bending reconstruction plate and miniplate (8.09±2.52 mm vs. 25.49±2.72 and 23.13±13.54 mm, respectively). When patient-specific plate was applied in vivo, the errors were not significantly different from the mock.
Conclusion
Patient-specific plates cooperated with CAS shows the least errors. Nevertheless, manually bent reconstruction plates and miniplates could be applied in limited defects with caution.
Evaluation of basalt powder against stored product insects
Authors: Melki Mohamed Nejib El , Barkouti Amal , Aoudi Wajdi , Moueddeb Khaled El , Beyaz Abdullah 
Published: January 2022

Abstract
The insecticidal effect of natural basalt powder on Tribolium castaneum was evaluated. Two tests were used to study the insecticidal action of the basalt powder on the stored durum wheat. The coating test followed by the Bioassay test. The coating test of Tribolium castaneum adults in 10g of natural basalt powder, for 17 samples at rate of 25 adults per sample and an uncoated control, showed an insecticidal effect of the said powder with mortality rate of 100% for 70 hours of exposure against 5% for the control sample with a homogeneity of the mortality rate for the 17 samples. The bioassay test was carried out on durum wheat, the natural basalt powder was applied as dust at six different doses. The results of showed an insecticidal efficacy closely related to the dose and the time of exposure. The 500ppm dose showed insecticidal potential after 12 days of exposure against Tribolium castaneum (100% mortality). However, the 300ppm dose showed significant efficacy (100% mortality) against Tribolium castaneum after 14 days of exposure. In addition, the doses over than or equal to 75ppm resulted in a significant reduction in the insect population (mortality more than 74% for 14 days of exposure) compared to the control, providing new approach of integrated pest management strategy.
Structural MRI Based Parcellation of The Human Brain Using Spatial Hierarchical Clustering
Authors: Tietz Tobias , Eickhoff Simon , Schwender Holger 
Published: January 2022

Abstract
Parcellating the human brain into neurobiologically meaningful regions serves two (key) purposes , i.e. to gain neurobiological insight on the modules of the brain and to achieve dimensionality reduction for large -scale studies . Data- driven parcellation approaches based on magnetic resonance imaging ( MRI ) mainly differ from each other by the modalities that they are derived from and the parcellation algorithm. Here we extensively evaluate parcellation approaches which are based on one specific modality , i.e. grey matter volume deduced from T1- weighted structural MRI scans . The newly proposed spatial hierarchical variable clustering method SPARTACUS is employed as parcellation algorithm and its performance is compared with other spatial hierarchical agglomerative clustering ( SHAC ) algorithms as well as with spatial spectral clustering on both simulated data and a single- site structural MRI data set of older adults using two metrics , i.e. clustering quality and clustering stability across subsamples . Our analysis reveals that SPARTACUS and Ward's SHAC outperform the other competing methods . Using spatial ensemble clustering methods we could further improve the quality of the parcellations . Performing parameter tuning of the granularity ( considering 2-1000 brain regions ) we identify multiple interesting numbers of brain regions , where the corresponding parcellations may reflect different levels of brain organization . Finally , we observe that our parcellations converge well above chance with other structural MRI based parcellations but hardly better or even worse than chance with parcellations from another modality .
The Pathological Spectrum of Pediatric Kidney Disease: 18-year Experience From a Single Tertiary Care Center in Northern Taiwan
Authors: Yen Chen-Wei , Chen Tai-Di , Yen Tzung-Hai , Yu Mei-Ching 
Published: January 2022

Abstract
Background: Glomerular disease is one of major causes leading to chronic kidney disease in children worldwide. The recent publications showed the changing spectrum of glomerular disease in certain countries. Therefore, we aimed to evaluate the histopathological patterns and change of pediatric kidney disease over the past eighteen years in northern Taiwan.Methods: This was a retrospective chart review study of the pediatric patients (≤ 18 years of age) undergoing percutaneous renal biopsies (PRBs) of native kidneys between January 2002 to July 2020 from a Pediatric Care Center at Chang Gung Memorial Hospital, Taoyuan, Taiwan.Results: A total of 339 pediatric native PRBs were analyzed in this study. The mean age of the subjects was 13.7 ± 7.0 years (184 girls and 155 boys). The most common indications of PRBs included acute nephritic syndrome (55.7%), idiopathic nephrotic syndrome (22.7%), persistent asymptomatic hematuria (13.9%) and unexplained renal failure (7.7%), respectively. Our study revealed proliferative lupus nephritis (LN), minimal change disease (MCD) related nephrotic syndrome and IgA nephropathy (IgAN) were the most frequent biopsy-proven pediatric glomerular diseases. Furthermore, we also found severe acute post-streptococcal glomerulonephritis (APSGN) was infrequent and even not be recognized after 2010.Conclusions: Our result disclosed the spectrum of biopsy-proven pediatric kidney disease was not significantly changed over the past two decades in northern Taiwan. LN, MCD and primary IgAN continued to be the most common histopathological diagnosis among Taiwanese children. We speculated multiple environmental and public health polices implemented in Taiwan might partly contribute to this unchanged pathological spectrum of pediatric kidney disease and reduced burden of infection-associated glomerular disease such as APSGN and pneumococcal-induced hemolytic uremic syndrome.