bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stratified Split Sampling of Electronic Health Records: Turning Early Science into Replicable Research
Authors: Huo Tianyao , Glueck Deborah , Shenkman Elizabeth , Muller Keith 
Published: November 2022

Abstract

Although superficially similar to data from clinical research, data extracted from electronic health records (EHRs) may require fundamentally different approaches to analysis and model building. Some outcome and predictor variables may not be well-defined at the start of the study. Selecting specific definitions requires exploratory data analysis. Specifying the rules for computing a new variable inevitably leads to exploratory analyses. Achieving replicability, i.e., a high probability that a similar future study will reach the same conclusions, requires special approaches. We recommend a study design strategy based on stratified sample splitting for studies using EHRs. The split-sample design ensures meeting the goal of replicability. Stratified sampling of EHRs increases generalizability by allowing heterogeneity between subgroups to be tested appropriately with good statistical power. Building a model from EHR data to predict uptake of hepatic cancer screening illustrates the recommended approach.
CD79A Work as a Potential Target For The Prognosis of Patients With HNSCC: Analysis of Immune Cell Infiltration In Head and Neck Squamous Cell Carcinoma Based on The CIBERSORTx Deconvolution Algorithm
Authors: Yao Shucong , Huang Zixian , Wei Changji , Wang Yuepeng , Xiao Hongwei , Chen Shisheng , Huang Zhiquan 
Published: November 2022

Abstract
Objective
To analyze the abundance of infiltrating tumor immune cells in patients with head and neck squamous cell carcinoma (HNSCC) and to search for potential targets that can predict patient prognosis.
Methods
A total of 400 samples from 210 patients with HNSCC were collected using The Cancer Genome Atlas (TCGA) database. CIBERSORTx was used to evaluate the infiltration abundance of tumor immune cells. Potential target genes were searched to predict patient prognosis through case grouping, differential analysis, and enrichment analysis. The correlation between target genes and tumor immune cell infiltration was verified using the TIMER2.0 database. Surgical excisional tissue sections of patients with head and neck squamous cell carcinoma admitted to the Department of Oral and Maxillofacial Surgery, Second Affiliated Hospital of Shantou University Medical College, from 2015 to 2018 were collected and followed up.
Results
The CIBERSORTx deconvolution algorithm was used to analyze the infiltration abundance of immune cells in the samples. Cases with a high infiltration abundance of naive and memory B lymphocytes exhibited a significantly improved prognosis. The prognosis of patients with high CD79A expression was significantly better than that of patients with low CD79A expression. In addition, CD79A expression was significantly correlated with B lymphocyte infiltration in the tumor microenvironment.
Conclusion
CD79A can predict the infiltration abundance of B lymphocytes in the tumor microenvironment of patients with HNSCC. CD79A is a potential target for predicting the prognosis of patients with HNSCC. This study provides novel ideas for the treatment of HNSCC and for predicting patient prognosis.
Cost Effectiveness Analysis of CAR-T Cell Therapy for Patients with Relapsed/Refractory Multiple Myeloma in China
Authors: Wu Weijia , Ding Shuyi , Zhang Mingming , Zhou Yuping , Sun Xueshan , Zhao Zixuan , Yang Yi , Hu Yongxian , Dong Hengjin 
Published: November 2022

Abstract
Background
The treatment of relapsed/refractory multiple myeloma (RRMM) dramatically changed with the emergence of chimeric antigen receptor T (CAR-T) cell therapy. The aim of this study was to evaluate the cost-effectiveness of two CAR-T cell treatments for RRMM patients from the perspective of the Chinese healthcare system.
Methods
Markov modelling was used to evaluate Idecabtagene vicleucel(Ide-cel) and Ciltacabtagene autoleucel (Cilta-cel) compared with currently available salvage chemotherapy for patients with RRMM over a lifetime horizon. The model was developed based on data from the three studies: CARTITUDE-1, KarMMa and MAMMOTH. Each CAR-T cell treatments was compared with currently available salvage chemotherapy. The healthcare cost and utility of RRMM patients were collected in a Chinese single institution. Main outcomes were life-years, discounted lifetime costs, discounted quality-adjusted life-years (QALYs), and incremental cost-effectiveness ratio.
Results
In the base case analysis, 3.5% and 30.4% of RRMM patients were expected to be long-term survivor after 5 years of Ide-cel and Cilta-cel treatment. Compared to salvage chemotherapy, Ide-cel and Cilta-cel were associated with the incremental QALYs of 1.14 and 3.32, and increment cost of US $166,643 and $111,225, leading to ICERs of $146,764 and $33,547 per QALY. In the scenario analyses, the ICER was $138,249 and $28,844 per QALY under assumption that the model starting age is changed from 60 to 55 for Ide-cel and Cilta-cel, and ICER was $148,486 and $40,691 per QALY under assumption that success rate of CAR-T therapy manufacturing was 100%.
Conclusions
Under the wiling-to-pay of 3 times China's per capita GDP in 2021, Cilta-cel was cost-effectiveness options compared to salvage chemotherapy for patients with RRMM while Ide-cel not. With younger target people, potential price discount and long-term survival improvement, the ICERs of the two CAR-T cell treatments would decrease.
Disentangle the elderly’s positive coping behavior in times of COVID-19: Perspectives from the social network, information acquisition, and perceived community support
Authors: Peng Chuanyu , Hu Hangyu , Yuan Guoping , Chen Junpeng , Mao Yanhui 
Published: November 2022

Abstract
Background
The global COVID-19 pandemic has devastatingly affected human well-being, especially for the elderly, who are more vulnerable to virus infection. However, scientific research and corresponding suggestions on how to help them cope positively with COVID-19 are emergently needed.
Method
Based on preliminary interviews with potential factors that may affect the elderly’s positive coping behavior, this study investigated 916 Chinese elderly (aged 60–96) and disentangled the impact of social network (i.e., network size, network strength, and network heterogeneity), information acquisition (i.e., depth and breadth of information), and perceived community support on the elderly’s COVID-19 positive coping behavior.
Results
Our results showed that social network (network strength and heterogeneity) and information acquisition are directly associated with positive coping behavior and indirectly through perceived community support.
Conclusions
Our findings enrich the theoretical literature on the elderly’s positive coping behavior in times of COVID-19 and provide new perspectives on how to help the elderly cope with health crises from the perspective of social network, information acquisition, and perceived community support.
Floral anatomy points to autogamy as the evolutionary path in Elatinaceae (Malpighiales)
Authors: Bonifácio Stéphani Karoline Vasconcelos , Amorim André M. , Oliveira Denise Maria Trombert 
Published: November 2022

Abstract
Since being established, the phylogenetic position of Elatinaceae has been controversial, but recent phylogenetic studies place it in Malpighiales as the sister group of Malpighiaceae. Molecular data support this relationship, but some morphological aspects of it are still poorly understood, especially floral evolution in Elatinaceae and its relationship with close lineages. Based on representatives of the two accepted genera in Elatinaceae, Bergia, and Elatine, we describe the floral anatomy of the family, compare it to that of the phylogenetically closest families, and discuss the relevance of anatomical floral characters. The presence of a glandular connective and hypostase are possible synapomorphies of the Elatinaceae + Malpighiaceae clade. The calyx glands present in Elatinaceae comprise cells with phenolic content without secretory activity. Some characters are autapomorphies for Elatinaceae, such as a reduction in the number of microsporangia and a thin-walled endothecium. However, other characteristics, such as a gamosepalous calyx, non-functional calyx glands, axile placentation, pendulous ovules, and intercarpellary vascular complexes, also occur in other families in the Malpighiales. The autapomorphies of Elatinaceae are evidence of structural adaptations of the flower in favor of autogamy, which could explain numerous morphological reversions. This aspect constitutes a marked difference in the Elatinaceae + Malpighiaceae clade since the latter is allogamous and Elatinaceae is autogamous, processes that are relevant to the evolutionary history of the two lineages.
Apoptosis in hypoxic mice influenced by miR-138-siRNAs-HIF-1α and miR- 21-siRNAs-HVCN1
Authors: Ijabi Janat , Roozehdar Parisa , Afrisham Reza , Moradi-Sardareh Heman , Polizzi Nicholas , Jasoni Christine L , Kaminsky Zachary A. , Ijabi Roghayeh , Tehranian Najmeh , Sadeghi Adel , Oraibi Bha-Aldan Mundher 
Published: November 2022

Abstract
Background
The complications of intraventricular-cerebral hemorrhage in premature infants are irreversible and epilepsy is common in these infants. Inflammation may cause damage to brain cells by increasing oxygen consumption, intracellular calcium, and acidosis. In an infant with intraventricular hemorrhage (IVH), the increase of HIF-1a and HVCN1can reduce the complication of oxygen consumption and acidosis as well as by decrease of S100B can protect nerve cells from apoptosis and epilepsy through less brain damage. In this study, we investigated apoptosis in hypoxic mice influenced by miR-138-siRNAs-HIF-1a and miR-21-siRNAs-HVCN1.
Methods
YKL40, HIF-1a, HVCN1, and S100b genes were compared between two groups of preterm infants with and without maternal inflammation on the firth and the third day of birth, and also they were followup up three months later to observe their seizures. Then, we transfected miRNAs into cell lines to detect the changes in YKL40, HIF-1a, HVCN1, and S100b genes expression and nerve cell apoptosis. By using specific siRNAs injected in mice, we increased the expression of HIF-1a and HVCN1 and decreased S100b genes. Changes in gene expression were assessed using real-time PCR, Western blotting, flow cytometry (FCM), and immunohistochemistry (IHC).
Results
The expression of the HVCN1 gene revealed a strong negative correlation with epilepsy in both groups of newborns (P < 0.001). The expression levels of the S100b, YKL40, and HIF-1a genes were significantly correlated with epilepsy (P < 0.001). By FCM, the apoptotic index (A.I.) was 41.6 ± 3.3 and 34.5 ± 5.2% after transfecting miRNA-431 and miRNA-34a in cell lines, respectively, while the A.I. was 9.6 ± 2.7 and 7.1 ± 4.2% after transfecting miRNA-21 and miRNA-138. By using IHC double-labeling, it was determined that when hypoxic mice received simultaneous injections of miR-138-siRNAs-HIF-1a and miR-21-siRNAs-HVCN1, there was less apoptosis and epilepsy than in the hypoxia group.
Conclusions
By injecting miR-138-siRNAs-HIF-1a and miR-21-siRNAs-HVCN1 simultaneously into hypoxia mice, we boosted HVCN1 and HIF-1a and decreased S100b, which reduced apoptosis and epilepsy in hypoxic mice.
Modelling and Analysis of Cyclone Separation Efficiency Based on Spacecraft Heat Pump System
Authors: Niu Zitian , Fu Zhendong , Yang Qi , Wu Qi , Yang Le , Miao Jianyin 
Published: November 2022

Abstract
The heat pump can effectively achieve high power and extremely high-temperature heat dissipation of manned spacecraft. The cyclone oil-gas separator is the core component of the heat pump system to separate lubricating oil and prevent it from entering the heat exchanger. In this paper, a one-dimensional separation efficiency calculation model is built based on the geometry of the spacecraft heat pump system's cyclone oil and gas separator, which can implement the fast calculation of the separation efficiency. At the same time, the structural parameters of the separator, the operating parameters and the physical properties of the oil-gas mixture entering into the separator are analyzed to obtain their influence rules on the separation efficiency, which can guide the design of the separator and the compressor's system operating conditions.
Total pancreatectomy for the treatment of grade C late post-pancreaticoduodenectomy hemorrhage
Authors: xu yong-fu , wang ai-dong , fang zheping , du xuefeng , li zhenyu , zhang caiming , zhang fabiao 
Published: November 2022

Abstract
Objective: Late post-pancreatoduodenectomy hemorrhage (LPPH) is associated with a significant increase in perioperative mortality. It is of great significance to explore effective treatment methods for severe LPPH patients.
Methods: Between 2014 and 2021, 224 pancreatoduodenectomies were performed, and 14 patients had a late hemorrhage (occurring >24 h postoperatively). The clinicopathological variables and treatments related to hemorrhage were investigated.
Results: Out of the 14 LPPH patients, 6 had grade B hemorrhage and 8 had grade C hemorrhage. There were 11 cases of comorbid pancreatic fistula, 5 cases of biliary fistula, and 7 cases of abdominal infection. The median time to first hemorrhage after surgery was 9 days. Of the 6 grade B LPPH patients, 4 underwent conservative treatment, 1 underwent transarterial embolization (TAE), and 1 underwent local suturing at the bleeding site for hemostasis. Of the 8 grade C LPPH patients, One patient without pancreatic fistula and abdominal infection was given conservative treatment after no bleeding was found by digital subtraction angiography examination. The other 7 patients had comorbid pancreatic fistula, of which 5 had comorbid abdominal infection, 3 had comorbid biliary fistula, and all 7 patients underwent total pancreatectomy of the residual pancreas. Three of these patients simultaneously underwent endovascular treatment. All patients recovered and were discharged, with a mortality of 0%.
Conclusion: Total pancreatectomy of the residual pancreas is safe and feasible in grade C LPPH patients with comorbid pancreatic fistula, biliary leakage, or abdominal infection.
IKAROS and AIOLOS activate AP-1 transcriptional complexes and are essential for natural killer cell development
Authors: Huntington Nicholas , Goh Wilford , Foroutan Momeneh , Scheer Sebastian , Pfefferle Aline , Sudholz Harrison , Shen Zihan , Delconte Rebecca , Hennesey Robert , Kong Isabella , Schuster Iona , Andoniou Chris , Souza-Fonseca-Guimaraes Fernando , Hediyeh-zadeh Soroor , Davis Melissa , Beavis Paul , Degli-Esposti Mariapia , Thiele Daniel , Kallies Axel , Cursons Joe , Rautela Jai , Nutt Stephen 
Published: November 2022

Abstract
Ikaros family transcription factors regulate lymphocyte biology and are targets of the immunomodulatory imide drugs (IMiDs) for hematological maligancies. Ikaros (Ikzf1/IKZF1) is the most broadly expressed family member in lymphocytes, yet its role in innate lymphopoiesis was unknown. Here we used conditional gene inactivation to reveal that Ikaros is required for normal NK cell development. Ikzf1-null NK cells had impaired IL-15 signaling, manifesting in reduced proliferation and enhanced apoptosis. Cish and Socs2, known negative regulators of IL-15 signaling are increased in Ikzf1-null NK cells and are direct targets of Ikaros-mediated repression. Ikzf1-null NK cells have extensive transcriptional alterations with a striking reduction in expression of genes encoding AP-1 transcriptional complexes as well as a compensatory increase in Ikaros-family members, Ikzf2 and Ikzf3. Deletion of both Ikzf1 and Ikzf3 in NK cells further reduced AP-1 gene expression culminating in a complete loss of peripheral NK cells in mice. Inactivation of Ikaros-family members in human NK cells also impaired their fitness and function, while genetic screens revealed a co-dependency on IKZF1 and individual AP-1 genes in hematopoietic cell survival, suggesting that IMiDs induce apoptosis of malignant IKZF1/3-dependent cells by ablating AP-1 transcriptional activity. Collectively we show the Ikaros-family are novel regulators of cytokine responsiveness and essential for promoting AP-1 transcriptional activity required for NK cell development.
Effects and safety of propofol intravenous anesthesia in transvaginal oocyte retrieval on outcomes of in vitro fertilization and embryo transplantation
Authors: Liu Xiao-ming , Zhang Fan , Zhong Hao-Yue , Lu Xiao-Sheng , Xie Zuo-Liang , Zhao Jun-Zhao , Xi Hai-Tao 
Published: November 2022

Abstract
Purpose
Propofol is a common anesthetic agent used for oocyte retrieval procedures to reduce the pain and fear in outpatients. However, the potential effects and safety of propofol on in vitro fertilization and embryo transfer (IVF-ET) is not clear yet.
Methods
A total of 1187 patients undergoing IVF-ET were recruited in this retrospective study and divided into two groups depending on whether they received propofol (propofol group, n = 140) or not (control group, n = 1047) for anesthesia during oocyte retrieval.
Results
Baseline information did not differ between the two groups (p > 0.05). Compared with control group, the number of oocytes retrieved in propofol group was more (p = 0.012), while both the estradiol (E2) level on the trigger day and the pre-ovulatory follicle count were higher in propofol group ((p < 0.01). Furthermore, the preterm delivery rate was higher in the propofol group (p < 0.001). To further analyze the effect of propofol on the oocyte retrieval rate, patients were divided into three subgroups depending on the pre-ovulatory follicle count (≤ 10, 11–20, and > 20) to eliminate the influence of inconsistency in the estimation of the pre-ovulatory follicle count between the two groups. Data showed that oocyte retrieval with propofol was beneficial for getting more oocytes in the pre-ovulatory follicle count 11–20 subgroup (p < 0.001).
Conclusion
Oocyte retrieval with propofol had no negative effect on fertilization, embryo quality, and clinical outcomes, and could raise the oocyte retrieval rate in patients with an estimated pre-ovulatory follicle count 11–20. These findings provided evidence for further clinical applications of this technique.
Short-term outcome of adrenal radiofrequency ablation of huge adrenal cysts: A single-center experience
Authors: Pak Shin Jeong , Lee Yu-mi , Kim Pyo Nyun , Kim Byung-Chang , Cho Jae Won , Kim Won Woong , Sung Tae-Yon , Chung Ki-wook , Hong Suck Joon 
Published: November 2022

Abstract
Percutaneous thermal ablation is a minimally invasive treatment for liver, kidney, lung, bone, and thyroid tumors. This treatment also has been used to treat adrenal tumors in patients, but there is no evidence for the efficacy of thermal ablation of adrenal cysts. The present study was performed to analyze the experience of a single center with percutaneous radiofrequency ablation (RFA) of huge adrenal cysts and to evaluate its efficacy. The present study enrolled all patients who underwent percutaneous RFA for unilateral adrenal cysts from 2019 to 2021. All patients underwent USG-guided percutaneous aspiration of cystic fluid, followed by RFA. A total nine patients with huge adrenal cysts were included in this study. All of them underwent technically successful percutaneous RFA, with no immediate complication. Follow-up CT 3 months after RFA showed that one patient had a poor response. She underwent secondary RFA with resulting in a good response. After a median follow-up period of 17.2 months, eight patients showed no evidence of recurrence. The patient, who showed evidence of recurrence, refused any other treatment and has been under regular surveillance. None of the nine patients developed adrenal insufficiency during the follow-up period. In conclusion, percutaneous RFA is a safe and effective minimally invasive treatment for huge adrenal cystic masses, suggesting that percutaneous RFA may be a good alternative option in selected patients.
Ab initio studies on complexes of ozone with triatomic and larger molecules.
Authors: Grein Friedrich 
Published: November 2022

Abstract
Using coupled cluster methods with quadruple-zeta basis sets stable structures were found for complexes of ozone with common triatomic molecules as well as with ammonia and methane. The largest dissociation energy of 917 cm− 1 was obtained for the hydrogen bonded O3-NH3 complex, exceeding the value for O3-H2O (787 cm− 1). The high dissociation energy of the O3-NH3 complex is seen as an exception to the expected sequence of stabilities. Large dissociation energies were obtained also for O3-SO2 (717 cm− 1), O3-N2O (688 cm− 1), O3-HCN (659 cm− 1) and O3-CO2 (652 cm− 1). Dissociation energies for the C1 structure (559 cm− 1) and Ci structure (556 cm− 1) of the ozone dimer are nearly identical despite pronounced differences in geometries. Hydrogen bonding of HF, H2O, NH3 and CH4 with ozone is contrasted to hydrogen bonding with the oxygen molecule. Large shifts in vibrational frequencies were found for the ozone dimer, with a red shift of 51 cm− 1 and a blue shift of 25 cm− 1 for the antisymmetric stretching band of ozone. Larger frequency shifts are also seen for complexes of ozone with NH3, HCN, H2O and N2O.
The inverse first-passage time problem as hydrodynamic limit of a particle system
Authors: Klump Alexander 
Published: November 2022

Abstract
Given a distribution on the positive real line, the two-sided inverse first-passage time problem for Brownian motion asks for a function, such that the first-passage time of this function by a reflected Brownian motion has the given distribution. We present a particle system without branching but with selection at timepoints depending on the given distribution on the positive real line, whose hydrodynamic limit is the distribution of a Brownian motion conditioned to not have passed the solution of the inverse first-passage time problem. As application we extract a simple Monte-Carlo method to simulate these solutions.
Effect of the geometric structures of mask feature on the microstructure forming in through-mask electrochemical micromachining
Authors: Wang Guoqian , Jiang Shan , Ni Shoudong , Zhang Yan 
Published: November 2022

Abstract

The manufacturing of surface microstructure is an important means for the modification of surface characteristics of parts. Through-mask electrochemical manufacturing is also an efficient way to fabricate surface microstructures. Nevertheless, in cases where the through-mask electrochemical manufacturing is used for the fabrication of microstructures, many experiments are required to control the cross-section morphology of the microstructures. Furthermore, there is a lack of quantitative analysis of the impact of key variables on the process. In this paper, using numerical simulations based on the geometric structure of mask feature, we analyze the effects of the aspect ratio of mask feature and the gap between cathode to mask on electrochemical micromachining. We further investigate the influence laws of the mask feature’s geometric structure on the anode current density distribution and forming process. A critical value of "0.8" is also determined for aspect ratio of mask feature which is closely related to the anode current density distribution and forming process. The through-mask electrochemical micromachining experiments confirm the effect laws of the geometric structure of the mask feature. And the critical value (0.8) of aspect ratio can be used as the basis for selecting mask feature.