bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of COVID-19 continuous containment and mitigation strategy on the epidemic of vector-borne diseases in China
Authors: Guo Xiangyu , Ma Chenjin , Wang Lan , Zhao Na , Liu Shelan , Xu Wangli 
Published: March 2022

Abstract
Background
This study explored the effect of a continuous mitigation and containment strategy for coronavirus disease 2019 (COVID-19) on five vector-borne diseases (VBDs) in China from 2020 to 2021.

Methods
Data on VBDs from 2015 to 2021 were obtained from the National Health Commission of the People’s Republic of China, and the actual trend in disease activity in 2020–2021 was compared with that in 2015–2019 using a two-ratio Z-test and two proportional tests. Similarly, the estimated trend in disease activity was compared with the actual trend in disease activity in 2020.

Results
There were 13,456 and 3684 average yearly cases of VBDs in 2015–2019 and 2020, respectively. This represents a decrease in the average yearly incidence of total VBDs of 72.95% in 2020, from 0.9753 per 100,000 population in 2015–2019 to 0.2638 per 100,000 population in 2020 (t = 75.17, P < 0.001). The observed morbidity rates of the overall VBDs were significantly lower than the predicted rates (47.04% reduction; t = 31.72, P < 0.001). The greatest decline was found in dengue, with a 77.13% reduction (observed rate vs predicted rate: 0.0574 vs. 0.2510 per 100,000; t = 41.42, P < 0.001). Similarly, the average yearly mortality rate of total VBDs decreased by 77.60%, from 0.0064 per 100,000 population in 2015–2019 to 0.0014 per 100,000 population in 2020 (t = 6.58, P < 0.001). A decreasing trend was also seen in the monthly incidence of total VBDs in 2021 compared to 2020 by 43.14% (t = 5.48, P < 0.001).

Conclusions
The results of this study verify that the mobility and mortality rates of VBDs significantly decreased from 2015–2019 to 2020–2021, and that they are possibly associated to the continuous COVID-19 mitigation and contamination strategy implemented in China in 2020–2021.

Graphical Abstract
Data mining of bulk and single-cell RNA sequencing introduces OBI1-AS1 as an astrocyte marker with possible role in glioma recurrence and progression
Authors: Mamivand Ali , Bayat Shiva , Maghrouni Abolfazl , Shabani Sasan , Khoshnevisan Alireza , Saffar Hiva , Tabrizi Mina 
Published: March 2022

AbstractLong non-coding RNAs (LncRNAs) are widely known for their various functions in cancer from tumor initiation to tumor progression and metastasis. Gliomas are the most prevalent primary forms of brain tumor, classified into grades I to IV according to their malignant histological features with grade IV, also known as glioblastoma multiforme (GBM), displaying the highest level of malignancy. Thus, the search for differentially expressed LncRNAs in GBM versus low-grade glioma to uncover new insights into the molecular mechanisms of glioma progression have intensified. Bulk RNA sequencing pinpointed decreased expression ofOBI1-AS1in GBM compared to low-grade glioma samples. Subsequent single nuclei RNA sequencing revealedOBI1-AS1to be a super-exclusive astrocyte marker with AUC = 0.99 and the potential to fully differentiate astrocytes from other brain cell types. Additional supplementary bioinformatics analysis exhibitedOBI1-AS1role in synaptic signal transduction and glutamatergic signaling. In addition, ChIP-Seq data were analyzed to explore transcription factors that can regulateOBI1-AS1expression in neural cells. Results of Hi-C, methylation and ChIP-Seq analysis strongly suggest methylation of the CTCF binding site serving a central role in regulation ofOBI1-AS1expression via managing chromatin interactions. Our study indicated that lncRNAs, likeOBI1-AS1,could be extremely precise in identifying the astrocyte cluster in the single-cell transcriptome and demonstrating superiority to well-established astrocyte markers such asGFAP,S100B,ALDH1L1, and AQP4.Graphical abstract
Improving the energy performance of the typical multi-family buildings in Amman, Jordan
Authors: Abu Qadourah Jenan , Al-Falahat Ala’a M. , Alrwashdeh Saad S. , Nytsch‐Geusen Christoph 
Published: March 2022

AbstractIn this paper, the possibility of reducing the energy demand of the typical multi-family buildings in Amman, Jordan utilizing passive design strategies are investigated through a parametric simulation study. Firstly, the energy demand of the multi-family buildings under the typical practice is evaluated, after that several design strategies are proposed such as wall and roof insulation, shading device, ventilation, etc. The effect of each strategy on the multi-family buildings’ energy demands is assessed alone and then in combination with the other strategies, to find the optimum solution to reduce the energy demand. This is beneficial for architects, engineers, and decisions makers involved in the design of energy-efficient multifamily buildings. The result proves that it’s possible to reduce the annual energy demand by 53, 71, and 78% of the cooling, heating, and lighting, respectively, by introducing passive design strategies. Around 45% savings of the heating demand are achieved due to adding wall insulation. Regarding the cooling demand about 17%, and 14% are saved due to applying a ventilated blind shading device and using a nighttime ventilation strategy.
Predation preference and nutritional values of four different aphid species for Orius sauteri (Hemiptera: Anthocoridae)
Authors: Zhang Liu , Li Tao , Qin Zifang , Cao Kaili , Gao Yifan , Wang Jingyi , Ge Yang , Shi Wangpeng 
Published: March 2022

Abstract
Background
Orius sauteri (Poppius) is one of the important natural enemies of aphids in Asia. Understanding its predation preference and efficiency can help improve its effectiveness as a biological control agent. Here, the predation preference of O. sauteri adults toward Aphis craccivora Koch, Rhopalosiphum maidis (Fitch), Myzus persicae Sulzer, and Aphis gossypii Glover was studied.

Results
The research found that O. sauteri had good predation efficiency on four important aphid species. Orius sauteri killed a similar number of four aphids in non-selective tests, but it preferred to eat more R. maidis and A. gossypii, and fewer A. craccivora in selective tests. The main biochemical components of the four aphid species were analyzed, including water content and nutritional components along with body mass. Rhopalosiphum maidis had a significantly lower water content than A. craccivora. Orius sauteri preferred R. maidis and A. gossypii, which harbored higher lipid and trehalose content, while preying on fewer A. craccivora, which had a larger body weight for the supplement of water and nutrition. The predation preference may be due to the closest nutrition composition to the optimal intake of O. sauteri.

Conclusions
This study showed that the predatory selection of O. sauteri was closely associated with the water content, bodyweight, lipid, and trehalose levels of prey, while the glycogen content and protein level might be less determinant. The high predation capacity on four aphids of O. sauteri indicated the great potential to integrate this predator into biological control strategies against aphids.
Study of the association between GLIS3 rs10758593 and type 2 diabetes mellitus in Egyptian population
Authors: Farid Mai Mohamed Mohamed , Abdel-Mageed Abeer Ibrahim , El-sherbini Aliaa , Mohamed Noha Refaat , Mohsen Manal 
Published: March 2022

Abstract
Background
GLIS3 (Gli-similar 3), a transcription factor, is involved in the maturation of pancreatic beta cells in fetal life, maintenance of cell mass as well as the control of insulin gene expression in adults. As a result, GLIS3 was reported as a susceptibility gene for type 1 diabetes, type 2 diabetes, and neonatal diabetes. Therefore, the goal of this study was to look into the association between the rs10758593 single nucleotide polymorphism (SNP) in the GLIS3 gene and T2DM in the Egyptian population.

Methods
Frequencies of the rs10758593 (A/G) SNPs were determined in 100 T2DM patients (cases) and in 100 non-diabetic healthy subjects (controls) using real-time PCR.

Results
The prevalence of the mutant genotypes, AA and AG, differed significantly between patients and controls. The AA genotype was more prevalent in the patients' group. The (AA) was found in 39% of the patients and 18% of the controls. While AG (heterozygous) genotype was found in 61% of the patients and 81% of the controls (p = 0.003). The AA genotype was significantly associated with T2DM. Moreover, The GLIS3 rs 10758593 mutation was found to be associated with the presence of diabetic retinopathy and nephropathy. In diabetic patients, a significant correlation between HbA1c with fasting glucose, fasting insulin, and HOMA-IR was found.

Conclusion
The rs10758593 polymorphism of the GLIS3 gene was found to be significantly associated with T2DM in an Egyptian population sample. Additionally, significant association between GLIS3 rs 10758593 mutation and the glycemic control was found.
Hematological indices in pediatric patients with acyanotic congenital heart disease: a cross-sectional study of 248 patients
Authors: Mohammadi Hanieh , Mohammadpour Ahranjani Behzad , Aghaei Moghadam Ehsan , Kompani Farzad , Mirbeyk Mona , Rezaei Nima 
Published: March 2022

Abstract
Background
Congenital heart disease CHD is a significant cause of mortality and morbidity in children worldwide. Patients with congenital heart disease may develop hematological problems, including thrombocytopenia and neutropenia. In addition, several studies indicate the higher frailty of patients with CHDs to infections and malignancies. Nevertheless, the mechanisms of immune system changes in these patients have remained in the shadow of uncertainty. Moreover, very few studies have worked on cytopenia in CHD. This study has assessed the frequency of thrombocytopenia, neutropenia, lymphopenia, and anemia in pediatric patients with acyanotic congenital heart disease ACHD prior to open-heart surgery.

Methods
This cross-sectional study was handled in the Pediatric Cardiology Clinic, Tehran University of Medical Sciences, during pre-operation visits from 2014 till 2019. Two hundred forty-eight children and adolescents with acyanotic congenital heart disease before open-heart surgery met the criteria to enter the study.

Results
A total of 191 (76.7%) patients with Ventricular Septal Defects (VSD), 37 (14.85%) patients with Atrial Septal Defects (ASD), and 20 (8.11%) patients with Patent Ductus Arteriosus (PDA) were enrolled in this study. The median age was 23.87 months. Thrombocytopenia and neutropenia were found, respectively, in 3 (1.2) and 23 (9.2%) patients. Hemoglobin level and lymphocyte count were significantly lower in patients with neutropenia than patients with normal neutrophil count (P value = 0.024 and P value = 0.000). Significant positive correlations were found between neutropenia and anemia. There were no correlations between neutrophil count and Platelets. Also, anemia was found in 48 patients (19.3%). The study also found a statistically significant correlation between the co-existence of VSD and neutropenia in the patients (P value = 0.000).

Conclusion
Although most were mildly neutropenic, there was a significant correlation between neutropenia and Ventricular Septal Defect compared to PDA and ASD groups. Regarding the importance of neutropenia to affect the prognosis of congenital heart defects in infections, it is important to consider further studies on the status of immune system function in these patients.
Association of methyltetrahydrofolate reductase gene mutation, homocysteine level with semen quality of Iraqi infertile males
Authors: Al-janabi Anwar Madlool , Al-Khafaji Salih Mahdi , Faris Shehab Ahmed 
Published: March 2022

Abstract
Background
Infertility is very common condition and almost 50% of cases are due to male factors. Several genetic and environmental factors are responsible for the poor quality and reduced number of sperms in several cases of infertility. The present study was designed to investigate the association between semen parameters, homocysteine, and the risk of C677T polymorphism of MTHFR gene in infertile males of Iraqi population.

Methods
This Case–control study has been conducted from February 2019 to July 2021 at a molecular laboratory in the Anatomy and Histology Department/college of Medicine/University of Kufa/Najaf/Iraq. It was composed of 353 infertile male patients. They were divided into five groups: 90 azoospermic, 84 oligospermia, 64 asthenospermic, 50 oligoasthenospermic, and 65 teratospermic with an age range 20–46 years compared with 100 fertile males as control with age range 21–49 years. In order to detect homocysteine levels, we used Hcy ELISA Kit. C677T mutation of MTHFR gene was employed by PCR–RFLP technique.

Results
Our data revealed three genotypes of MTHFR C677T, 167 (47.3%) subjects had CC genotype, 116 (32.9%) subjects had CT genotype and 70 (21.1%) subjects had TT genotype. Furthermore, T allele was associated with higher risk of infertility in all patients groups for any genetic model. In total infertile subjects (codominant model: CT vs. CC, OR = 2.0, 95% C.I = 1.2–3.3, P = 0.011; TT vs. CC, OR = 4.8, 95% C.I = 3.3–8.2, P = 0.0003; dominant model: CT + TT vs. CC, OR = 2.8, 95% C.I = 1.7–4.5, P = 0.0001). Oligoasthenospermic patients associated with higher risk in CT heterozygous genotype (OR = 2.8, 95% C.I = 1.0–4.9, P = 0.03) and TT homozygous of mutant allele (OR = 6.3, 95% C.I = 1.9–9.2, P = 0.002). Homocystein level was elevated in all infertile groups when compared with control group (P < 0.01), but the elevation was marked in oligoasthenospermia group. As well as, the level of Serum Hcy exhibited the highest value in TT mutant genotype (39.7 µmol/ml) followed by CT genotype (28.5 µmol/ml) while the lowest level of Hcy recorded in CC genotype (14.6 µmol/ml) for oligoasthenospermia group.

Conclusions
By relating the MTHFR C677T gene mutation with a higher homocystein level, the results showed that Iraqi males with this mutation are more likely to suffer from infertility.
Occurrence of gastrointestinal (GI) parasites in captive Olive Baboon and Common Langur in Bangladesh
Authors: Tabasshum Taniza , Liza Fahmida Tasnim , Rabbe Md. Fazle , Mukutmoni Mandira , Alam Md. Mahabub , Begum Aleya 
Published: March 2022

AbstractNon-human primates (NHPs) serve as necessary reservoir hosts of parasites that create diseases to human. A close interaction between human and NHP can make a pathway for transmission of zoonotic diseases. To prevent zoonotic infection of zoo keepers, park visitors as well as keeping the captive NHPs in healthy state, it is necessary to carry out regular parasitological examination and treatment. A total of 72 fecal samples of Olive Baboon (n = 39) and Common Langur (n = 33) irrespective of their age and sex were collected from two zoological gardens of Bangladesh. Eggs and oocysts of seven gastrointestinal (GI) parasites were observed and identified in samples of both host species. The prevalence of GI parasites recorded was 100%. In case of Olive Baboon, the protozoan prevalence was higher (53.83%) than that of helminths, but opposite scenario was seen in case of Common Langur. Besides, higher intensity of coccidian oocysts in both hosts was recorded in the study.
Evolving Effect of the COVID-19 Pandemic on Cancer-Related Encounters
Authors: London Jack W. , Fazio-Eynullayeva Elnara , Palchuk Matvey B. , McNair Christopher 
Published: March 2022

PURPOSE This is an update to a previously published report characterizing the impact that efforts to control the COVID-19 pandemic have had on the normal course of cancer-related encounters. METHODS Data were analyzed from 22 US health care organizations (members of the TriNetX global network) having relevant, up-to-date encounter data. Although the original study compared encounter data pre–COVID-19 (January-April 2019) with the corresponding months in 2020, this update considers data through April 2021. As before, cohorts were generated for all neoplasm patients (malignant, benign, in situ, and of unspecified behavior), all new incidence neoplasm patients, exclusively malignant neoplasm patients, and new incidence malignant neoplasm patients. Data on the initial cancer stage were available for calendar year 2020 from about one third of the study's organizations. RESULTS Although COVID-19 cases fluctuated through 2021, newly diagnosed cancers closely paralleled the prepandemic base year 2019. Similarly, screening for breast, colorectal, and cervical cancers quickly recovered beginning in May 2020 to prepandemic numbers. Preliminary data for the initial cancer stage showed no significant difference ( P > .10) in distribution for breast or colon cancers between 2019 and 2020. CONCLUSION Although the number of COVID-19 cases fluctuated, the steep declines observed during March and April 2020 in screening for breast and colon cancer and patients with newly diagnosed cancer did not continue through the rest of 2020 and into April 2021. Screening and new incidence cancer numbers quickly rose compared with prepandemic levels. The concern that more patients with advanced-stage cancer would be seen in the months following the drastic dips of March-April 2020 was not realized as the major disruption to normal cancer care was limited to these 2 months.
Cancer Burden Among Arab-World Females in 2020: Working Toward Improving Outcomes
Authors: Mahdi Hala , Mula-Hussain Layth , Ramzi Zhian S. , Tolba Marwan , Abdel-Rahman Omar , Abu-Gheida Ibrahim , Khorshid Ola , Al Sukhun Sana , Siddiqi Nadeem P. , Al Mandhari Zahid , Al Hussaini Maysa 
Published: March 2022

PURPOSE Cancer is the leading cause of morbidity and mortality worldwide. This work presents the Arab-world females' cancers (AFCs) statistics in 2020, compared with the 2018 AFCs statistics, the Arab-world male cancers statistics, and the world females' cancers (WFCs) statistics in 2020. This can help set the stage for a better policy for cancer control programs and improve outcomes. MATERIALS AND METHODS A descriptive review of the 2020 Global Cancer Observatory concerning AFCs was performed. Data on various cancers were compiled and compared among the countries in the region and WFCs. RESULTS A total estimate of 244,317 new cases and 132,249 deaths is reported in AFCs; representing 2.65% and 2.99% of WFCs, respectively, with an average crude incidence/mortality ratio of 116.2 (/100,000 population)/62.9 (/100,000 population) and an age-standardized incidence/mortality ratio of 137.7(/100,000 population)/77.2(/100,000 population) compared with 238.8(/100,000 population)/114.6(/100,000 population) and 186(/100,000 population)/84.2(/100,000 population) of WFCs, respectively. Five-year prevalent cases were 585,295; 2.28% of WFCs. In comparison to males, females accounted for 47.8% of the whole population, 52.9% in incidence, 46.9% in mortality, and 56.9% in the prevalence of patients with cancer. Mortality-to-incidence ratio (MIR) was 0.54 (range 0.39-0.62 in Arab countries, compared with 0.48 globally), and it ranged from 0.14 to 0.97 in the 30 AFC types. Breast cancer was the most common cancer in incidence and mortality, with an MIR of 0.39. CONCLUSION The 2020 descriptive analysis of the females' cancers in the Arab world revealed a relatively high MIR compared with females' cancers worldwide; a lower MIR compared with the males; and comparable MIR to 2018 one. We call for more in-depth studies to determine the causes of these differences that might translate into actionable interventions and better outcomes.
Temozolomide Followed by Combination With Low-Dose Ipilimumab and Nivolumab in Patients With Microsatellite-Stable, O6-Methylguanine–DNA Methyltransferase–Silenced Metastatic Colorectal Cancer: The MAYA Trial
Authors: Morano Federica , Raimondi Alessandra , Pagani Filippo , Lonardi Sara , Salvatore Lisa , Cremolini Chiara , Murgioni Sabina , Randon Giovanni , Palermo Federica , Antonuzzo Lorenzo , Pella Nicoletta , Racca Patrizia , Prisciandaro Michele , Niger Monica , Corti Francesca , Bergamo Francesca , Zaniboni Alberto , Ratti Margherita , Palazzo Michele , Cagnazzo Celeste , Calegari Maria Alessandra , Marmorino Federica , Capone Iolanda , Conca Elena , Busico Adele , Brich Silvia , Tamborini Elena , Perrone Federica , Di Maio Massimo , Milione Massimo , Di Bartolomeo Maria , de Braud Filippo , Pietrantonio Filippo 
Published: March 2022

PURPOSE This is a multicenter, single-arm phase II trial evaluating the efficacy and safety of an immune-sensitizing strategy with temozolomide priming followed by a combination of low-dose ipilimumab and nivolumab in patients with microsatellite-stable (MSS) and O6-methylguanine–DNA methyltransferase (MGMT)–silenced metastatic colorectal cancer (mCRC). PATIENTS AND METHODS Patients with pretreated mCRC were centrally prescreened for MSS status and MGMT silencing (ie, lack of MGMT expression by immunohistochemistry plus MGMT methylation by pyrosequencing). Eligible patients received two priming cycles of oral temozolomide 150 mg/sqm once daily, days 1-5, once every 4 weeks (first treatment part) followed, in absence of progression, by its combination with ipilimumab 1 mg/kg once every 8 weeks and nivolumab 480 mg once every 4 weeks (second treatment part). The primary end point was the 8-month progression-free survival (PFS) rate calculated from enrollment in patients who started the second treatment part, with ≥ 4 out of 27 subjects progression-free by the 8-month time point as decision rule. RESULTS Among 716 prescreened patients, 204 (29%) were molecularly eligible and 135 started the first treatment part. Among these, 102 (76%) were discontinued because of death or disease progression on temozolomide priming, whereas 33 patients (24%) who achieved disease control started the second treatment part and represented the final study population. After a median follow-up of 23.1 months (interquartile range, 14.9-24.6 months), 8-month PFS rate was 36%. Median PFS and overall survival were 7.0 and 18.4 months, respectively, and overall response rate was 45%. Grade 3-4 immune-related adverse events were skin rash (6%), colitis (3%), and hypophysitis (3%). No unexpected adverse events or treatment-related deaths were reported. CONCLUSION The MAYA study provided proof-of-concept that a sequence of temozolomide priming followed by a combination of low-dose ipilimumab and nivolumab may induce durable clinical benefit in MSS and MGMT-silenced mCRC.