bluuebunny/crossref_metadata_2025_split huggingface dataset:
GPRASP1 is a candidate anti‐oncogene and correlates with immune microenvironment and immunotherapeutic efficiency in head and neck cancer
Authors: Zhang Tao , Liu Genglong , Zhang Juan , Chen Shuaijun , Deng Zeyi , Xie Minqiang 
Published: October 2022

AbstractBackgroundG‐protein‐coupled receptor‐associated sorting protein 1 (GPRASP1) plays an important role in tumorigenesis. However, GPRASP1 specific role has not been clarified in head and neck cancer (HNC).MethodsHNC RNA sequencing (RNA‐seq) datasets, DNA methylation data, somatic mutation data, copy number variation (CNV) data, and corresponding clinicopathologic information were acquired from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. A comprehensive evaluation was performed to explore the relationship of GPRASP1 expression with clinicopathologic characteristics, CNV, and DNA methylation. Additionally, we employed HNC tissue microarray (TMA) to further confirm the relation between GPRASP1 expression and clinical features. Then, we systematically associated the GPRASP1 with immunological properties from numerous perspectives, such as immune cell infiltration, immune‐related pathways, immune checkpoint inhibitors (ICIs), immunomodulators, immunogenicity, and immunotherapy.ResultsAnalyzing TCGA, GEO, and TMA datasets, GPRASP1 is significantly down‐regulated in HNC compared to normal tissues. The expression of GPRASP1 is significantly negatively correlated with clinical features (perineural invasion, histologic grade, T stage, and TNM stage), and is an independent predictor of favorable prognosis, regardless of other clinicopathological features (HR: 0.42, 95% CI 0.20–0.91, p = 0.028). The etiological investigation found that the abnormal expression of GPRASP1 was related to DNA methylation, not CMV. Subsequently, the high expression of GPRASP1 was significantly correlated with immune cell infiltration (CD8+ T cell, tumor infiltrating lymphocyte), immune‐related pathways (cytolytic activity, check‐point, human leukocyte antigen), ICIs (CTLA4, HAVCR2, LAG3, PDCD1, and TIGIT), immunomodulators (CCR4/5, CXCL9, CXCR3/4/5), and immunogenicity (immune score, neoantigen, tumor mutation burden). Finally, immunophenoscore and tumor immune dysfunction and exclusion analysis demonstrated that GPRASP1 expression levels can accurately predict the immunotherapeutic response.ConclusionGPRASP1 is a promising candidate biomarker that plays a role in the occurrence, development, and prognosis of HNC. Evaluating GPRASP1 expression will aid in the characterization of tumor microenvironment infiltration and orient more efficient immunotherapy strategies.
Uses of Health Care System Medical Care Services by Athletes After Injury at the High School Level
Authors: Bullock Garrett S. , Mobley John F. , Brooks John M. , Rauh Mitchel J. , Gil Gilliland R. , Kissenberth Michael J. , Shanley Ellen 
Published: October 2022

AbstractBackgroundHealth care utilization can vary by age group, geographic location, and socioeconomic status (SES). A paucity of information exists regarding the availability and utilization of medical care by injured scholastic athletes. The purpose of this study was to describe and compare injuries and health care service utilization by school SES over an academic year.MethodsInjury and health care service data was collected from 1 large school district. Percentage of free and reduced lunch (FRPL) for each school was calculated to stratify schools into high (<50% FRPL) and low (≥50.1% FRPL) SES groups. Incidence proportion and relative risk (RR) with 95% confidence intervals (95% CI) were calculated.ResultsAbout 1756 injuries were reported among over 7000 participating athletes from 14 high schools. Similar injury incidence proportions were reported between high and low SES schools (RR = 1.10 [1.00–1.20]). Athletes from low SES schools were twice (RR = 2.01 [1.21–3.35]) and over three (RR = 3.42 [1.84–6.55]) times more likely to receive emergency and physical therapy care. SES was not associated with the use of physician, imaging, or surgery services.Implications for School Health, Policy, and EquitySchool medical providers and administrators should have ready and provide a list of trusted outside primary care and specialty providers that have experience in sports medicine. They should also enquire and follow up on which outside provider the high school athlete will seek care when referring out to outside providers.ConclusionsInjury incidence was similar between high and low SES schools. However, athletes from low SES high schools were over 2‐fold more likely to use emergency department services. Understanding factors influencing health care services choice and usage by student athletes from different socioeconomic backgrounds may assist sport medicine clinicians in identifying barriers and potential solutions in improving time to health restoration, athlete outcomes, and health care monetary burden.
Recent topics of neuronal intranuclear inclusion disease (NIID)
Authors: Sone Jun 
Published: October 2022

AbstractNeuronal intranuclear inclusion disease (NIID) is a neurodegenerative disease in which intranuclear inclusions are widely observed in the central and peripheral nervous systems and cells of general organs. For many years, it was diagnosed at autopsy. But in 2011, it became possible to diagnose NIID by skin biopsy, and in 2019, the expansion of the NOTCH2NLC GGC repeat was identified as the cause of NIID. Many NIID cases are now diagnosed antemortem by skin biopsy and genetic tests, and the clinical features of NIID are analyzed and reported. In addition, the causes of many repeat diseases have been identified. For example, Fragile‐X‐associated tremor/ataxia syndrome and oculopharyngodistal myopathy have been reported to show clinical and laboratory findings similar to NIID during their course. It is desirable to analyze the expansion of the NOTCH2NLC GGC repeat in order to distinguish it from other diseases. It is necessary to reorganize the disease concept of each disease through genetical assessment.
Cerebral air embolism: A hidden cause of acute ischemic stroke
Authors: Zivelonghi Cecilia , De Pasqual Carlo Alberto , Plebani Mauro , Cappellari Manuel 
Published: October 2022

AbstractA 46‐year‐old man presented to the Emergency Department because of food bolus impaction after an emergent esophagogastroduodenoscopy (EGD). Three hours after the procedure, awakening from general anesthesia, he complained drowsiness and left‐sided weakness. Brain CT scan was unremarkable, while CT angiography performed later revealed signs of pneumomediastinum likely from an esophageal laceration. Thorough cardiological, hematological, and rheumatological investigations during hospital stay proved unremarkable. Cerebral air embolism (CAE) has been reported as a potential complication of endoscopic procedures, but the precise mechanism is still unknown.
Diversity and divergence: evolution of secondary metabolism in the tropical tree genus Inga
Authors: Forrister Dale L. , Endara María‐José , Soule Abrianna J. , Younkin Gordon C. , Mills Anthony G. , Lokvam John , Dexter Kyle G. , Pennington R. Toby , Kidner Catherine A. , Nicholls James A. , Loiseau Oriane , Kursar Thomas A. , Coley Phyllis D. 
Published: October 2022

Summary
Plants are widely recognized as chemical factories, with each species producing dozens to hundreds of unique secondary metabolites. These compounds shape the interactions between plants and their natural enemies. We explore the evolutionary patterns and processes by which plants generate chemical diversity, from evolving novel compounds to unique chemical profiles.
We characterized the chemical profile of one‐third of the species of tropical rainforest trees in the genus Inga (c. 100, Fabaceae) using ultraperformance liquid chromatography‐mass spectrometry‐based metabolomics and applied phylogenetic comparative methods to understand the mode of chemical evolution.
We show: each Inga species contain structurally unrelated compounds and high levels of phytochemical diversity; closely related species have divergent chemical profiles, with individual compounds, compound classes, and chemical profiles showing little‐to‐no phylogenetic signal; at the evolutionary time scale, a species' chemical profile shows a signature of divergent adaptation. At the ecological time scale, sympatric species were the most divergent, implying it is also advantageous to maintain a unique chemical profile from community members; finally, we integrate these patterns with a model for how chemical diversity evolves.
Taken together, these results show that phytochemical diversity and divergence are fundamental to the ecology and evolution of plants.
The RhLOL1–RhILR3 module mediates cytokinin‐induced petal abscission in rose
Authors: Jiang Chuyan , Liang Yue , Deng Shuning , Liu Yang , Zhao Haohao , Li Susu , Jiang Cai‐Zhong , Gao Junping , Ma Chao 
Published: October 2022

Summary
In many plant species, petal abscission can be considered the final step of petal senescence. Cytokinins (CKs) are powerful suppressors of petal senescence; however, their role in petal abscission is ambiguous.
Here, we observed that, in rose (Rosa hybrida), biologically active CK is accumulated during petal abscission and acts as an accelerator of the abscission process. Using a combination of reverse genetics, and molecular and biochemical techniques, we explored the roles of a LESION SIMULATING DISEASE1 (LSD1) family member RhLOL1 interacting with a bHLH transcription factor RhILR3 in CK‐induced petal abscission.
Silencing RhLOL1 delays rose petal abscission, while the overexpression of its ortholog SlLOL1 in tomato (Solanum lycopersicum) promotes pedicel abscission, indicating the conserved function of LOL1 in activating plant floral organ abscission. In addition, we identify a bHLH transcription factor, RhILR3, that interacts with RhLOL1. We show that RhILR3 binds to the promoters of the auxin signaling repressor auxin/indole‐3‐acetic acid (Aux/IAA) genes to inhibit their expression; however, the interaction of RhLOL1 with RhILR3 activates the expression of the Aux/IAA genes including RhIAA4‐1. Silencing RhIAA4‐1 delays rose petal abscission.
Our results thus reveal a RhLOL1–RhILR3 regulatory module involved in CK‐induced petal abscission via the regulation of the expression of the Aux/IAA genes.
PBS3: a versatile player in and beyond salicylic acid biosynthesis in Arabidopsis
Authors: Li Wei , He Jinyu , Wang Xiuzhuo , Ashline Matthew , Wu Zirui , Liu Fengquan , Fu Zheng Qing , Chang Ming 
Published: October 2022

SummaryAVRPPHB SUSCEPTIBLE 3 (PBS3) belongs to the GH3 family of acyl acid amido synthetases, which conjugates amino acids to diverse acyl acid substrates. Recent studies demonstrate that PBS3 in Arabidopsis plays a key role in the biosynthesis of plant defense hormone salicylic acid (SA) by catalyzing the conjugation of glutamate to isochorismate to form isochorismate‐9‐glutamate, which is then used to produce SA through spontaneous decay or ENHANCED PSEUDOMONAS SUSCEPTIBILITY (EPS1) catalysis. Consistent with its function as an essential enzyme for SA biosynthesis, PBS3 is well known to be a positive regulator of plant immunity in Arabidopsis. Additionally, PBS3 is also involved in the trade‐off between abiotic and biotic stress responses in Arabidopsis by suppressing the inhibitory effect of abscisic acid on SA‐mediated plant immunity. Besides stress responses, PBS3 also plays a role in plant development. Under long‐day conditions, PBS3 influences Arabidopsis flowering time by regulating the expression of flowering regulators FLOWERING LOCUS C and FLOWERING LOCUS T. Taken together, PBS3 functions in the signaling network of plant development and responses to biotic and/or abiotic stresses, but the molecular mechanisms underlying its diverse roles remain obscure.
The impact of climate action on energy security in West Africa: Evidence from Burkina Faso, Ghana and Nigeria
Authors: Ofosu‐Peasah Gideon , Antwi Eric Ofosu , Blyth William , Sarquah Khadija 
Published: October 2022

AbstractThe study investigates the impact of climate action on energy security in West Africa using an autoregressive distributed lag and an error correction model. Empirical results for Burkina Faso showed that total carbon emissions and climate finance improved energy security performance in the country in the short and long run, while capacity building and energy efficiency impaired energy security performance in the short run. In the long run, capacity‐building efforts are estimated to improve energy security performance in Burkina Faso. For Ghana, results showed that total carbon emissions improved energy security performance in Ghana while energy efficiency impaired energy security performance in the short run. Results for climate finance and capacity building are found to be insignificant, and no long‐run results are found for Ghana. Lastly, results for Nigeria showed that total carbon emissions improved energy security performance in the long run. The short‐run result for total carbon emissions and climate finance is found to be insignificant, while capacity building and energy efficiency impaired energy security performance in the long and short run. Considering the need to reduce carbon emissions during full implementation of Nationally Determined Contributions, policies should aim at decoupling carbon emissions from energy security by exploring options for low‐carbon energy development.
Incidence of superficial left hepatic vein and its usability for graft hepatic vein venoplasty in pediatric liver transplantation
Authors: Namgoong Jung‐Man , Ageel Amro Hasan , Hwang Shin , Ha Tae‐Yong , Song Gi‐Won , Jung Dong‐Hwan , Park Gil‐Chun , Ahn Chul‐Soo , Moon Deok‐Bog , Tak Eunyoung , Kim Kyoung‐Mo , Oh Seok‐Hee 
Published: October 2022

AbstractBackgroundsThe anatomy of the left hepatic vein (LHV) is variable; thus, it should be considered for graft hepatic vein (GHV) venoplasty for left lateral section (LLS) and left liver grafts. This study assessed the incidence of superficial LHV (sLHV) branches according to LHV anatomy and its usability for GHV venoplasty in pediatric liver transplantation (LT).MethodsThis study consisted of three parts: (1) anatomical classification of LHV variations and the incidence of sLHV branches; (2) morphometric simulative analysis of GHV reconstruction and (3) clinical application based on LHV anatomy.ResultsThe LHV anatomy of 248 potential LLS graft donors was classified into four types according to the number and location of GHV openings: one single opening (type 1; n = 186 [75.0%]), two large openings (type 2; n = 35 [14.1%]), one large and one small adjacent opening (type 3; n = 14 [5.6%]), and two large widely‐separated openings (type 4; n = 13 [5.2%]). An sLHV branch was identified in 87 of 248 (35.1%) donor livers. Morphometric analysis of simulative GHV venoplasty with an sLHV branch increased GHV diameters by 30% in type 1 LLS grafts and 20% in type 2/3 LLS grafts. An analysis of 50 consecutive patients who underwent pediatric LT showed that the 2‐year rates of GHV obstruction were 2.0% with LLS grafts and 0% with left liver grafts.ConclusionsThe GHV orifice can be enlarged through LHV anatomy‐based unification venoplasty. Unification venoplasty with an sLHV branch provided sufficient enlargement of the GHV orifice.
Comparison of ultrasonography, progesterone assay, ballottement, and non‐return‐to‐heat with digital rectal palpation technique for early pregnancy diagnosis in Red Sokoto goat
Authors: Bello Adeh A. , Voh Ambrose A. , Ogwu David , Tekdek Lazarus B. , Ayo Joseph O. 
Published: October 2022

AbstractThe objective of the study was to evaluate the effectiveness of the digital rectal palpation (DRP) technique for early pregnancy diagnosis and to compare the results of experiments I and II in the Red Sokoto goat. Experiment I had 68 goats with 52 does and 16 bucks. Does were divided into prostaglandin F2‐alpha (PGF2α; n = 18), progesterone pessaries (P4P; n = 18), and Control (n = 16) groups as oestrus synchronizaton methods. Bucks were used for oestrus detection (n = 6) and breeding (n = 10). Comparative pregnancy diagnosis was carried out on day 21 post‐breeding using ultrasonography (US), DRP, progesterone assay (PA), ballottement (BL), and non‐return‐to‐heat (NRH). Experiment II was a repeat but had 51 animals with 42 does and nine bucks; three bucks for oestrus detection and six for breeding. Does were divided into recto‐vaginal artificial insemination (AI) by DRP (n = 14), vaginal speculum AI (n = 14), and Control‐natural service (n = 14) groups, indicating breeding methods. Oestrus was synchronized with PGF2α and pregnancy diagnosis was carried out on day 21 using US, DRP, PA, and NRH. The results for both experiments were similar. Pregnancy rates for PGF2α group were 66.2%, 66.7%, 64.8%, 62.1%, and 63.0% for US, DRP, PA, BL, and NRH, respectively; P4P had 81.5%, 81.5%, 42.6%, 20.3%, and 42.6% for US, DRP, PA, BL, and NRH, respectively; while Control was 73.6%, 79.2%, 70.9%, 73.6%, and 73.6% for US, DRP, PA, BL, and NRH, respectively. Proportions are significantly (p < .000) different in the P4P group. It was concluded that the DRP technique was effective and consistent in early pregnancy diagnosis on day 21 and comparable to US, PA, BL, and non‐return‐to‐oestrus in both experiments in Red Sokoto goat does.
Sub‐Saharan Africa's rising public debt stock: Is there a cause for concern?
Authors: Olaoye Olumide Olusegun , Olomola Phillip A. 
Published: October 2022

AbstractThe study investigated public debt sustainability in sub‐Saharan Africa (SSA) by testing the reaction of the primary balance to positive and negative shocks in public debts in a panel of 45 SSA countries. The study adopts the innovative nonlinear fiscal reaction function and the dynamic panel threshold model to account for the potential asymmetric phenomenon in the public debt series. In line with extant studies, the study found that public debts in SSA are weakly sustainable and there is a highly procyclical fiscal policy bias in SSA countries, particularly in resource‐rich countries, indicating that governments' fiscal policy responses are expansionary during economic upturns and contractionary during recessions, which may aggravate recessions and worsen debt situations across SSA. For robustness, the study compares the results with emerging and developed economies. The results indicate that in advanced economies, public debt is sustainable and that fiscal policy response is countercyclical. The research and policy implications are discussed.
Moral emotions in early childhood: Validation of the Chinese moral emotion questionnaire
Authors: Li Zijian , Li Boya , Tsou Yung‐Ting , Oosterveld Paul , Rieffe Carolien 
Published: October 2022

AbstractMoral emotions such as pride, guilt and shame play an important role in the social‐emotional development of preschool children. However, there are not many instruments available for measuring moral emotions in the preschool age. Moreover, relatively few research had examined cross‐cultural validity of measures for moral emotions. The present study tested the Chinese version of the Moral emotion questionnaire (MEQ) upon a group of (N = 182) Chinese preschool children aged from 2 to 6 years. The Chinese MEQ is a parent‐report translated from Dutch, assessing behavioural responses of pride, guilt and shame in preschool children. Confirmatory factor analysis showed satisfactory goodness‐of‐fit indexes for a three‐factor structure (Pride, Guilt, Shame) with 15 loading items. For concurrent relations, the results suggested an adaptive role of pride and guilt and a maladaptive role of shame in the social‐emotional development of preschool children. We could conclude that the 15‐item Chinese MEQ is a valid and reliable instrument for measuring pride, guilt, and shame in 2–6‐year‐old children in the Chinese context.
Female sex and advanced age are associated with invasive cytomegalovirus disease in solid organ transplant recipients
Authors: Perry Whitney A. , Gardiner Bradley J. , Price Lori Lyn , Rodriguez‐Garcia Marta , Chow Jennifer K. , Snydman David R. 
Published: October 2022

AbstractBackgroundLimited data exist to describe sex‐based differences in the severity of cytomegalovirus (CMV) infection after solid organ transplant (SOT). We sought to identify if a difference exists in likelihood of tissue‐invasive disease between male and female SOT recipients and to understand how age affects this relationship.MethodsA retrospective cohort of 180 heart, liver, and kidney recipients treated for CMV was examined. A logistic regression model was developed to assess the relationship between female sex and CMV type (noninvasive vs. invasive). A secondary regression analysis looked at the relationship of invasive CMV with a variable combining sex with age above or below 50.ResultsThere were 37 cases of proven or probable invasive CMV, occurring in 30% of females versus 16% of males. After adjustment for potential confounders, females with CMV infection were significantly more likely to have invasive disease (odds ratio (OR) 2.69, 95% confidence interval (CI) 1.25–5.90, p = .01). Females 50 years or older were at particular risk compared with males under 50 years (adjusted OR 4.54, 95% CI 1.33–18.83, p = .02).ConclusionFemale SOT recipients with CMV in our cohort were more likely than males to have tissue‐invasive disease, with the highest risk among older females. Further prospective studies are warranted to explore underlying immunologic mechanisms.
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Effects of subbase materials on phosphorous attenuation in permeable pavement system and correlation between pollution attenuation and subbase materials
Authors: Kuruppu Upeka , Rahman Ataur 
Published: October 2022

AbstractIn this study, laboratory models of six permeable pavement system (PPS) columns with various subbase configurations were examined for phosphorus attenuation. Experiments were carried out under varying intensities and durations of rainfall to investigate possible PPS modifications to enhance the performance of phosphorus attenuation and the likelihood of phosphorus leaching back to infiltration. The results show the inefficiencies of phosphate attenuation in the conventional PPS structure. During long‐duration rainfall events, phosphates can be leached back to the infiltrate through the conventional PPS structure. The intensity of rainfall affects the attenuation of total phosphorus through the six permeable pavement columns. To achieve an enhanced phosphate attenuation, the findings of this study suggest modifying the PPS structure by incorporating thin sand layers in the subbase. Also, the correlation between pollutant attenuation and subbase materials is found. Finally, design modification of PPS is recommended to enhance the pollutant attenuation capacity of the PPS.