bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of regulation of medically assisted reproduction on the risk of hospitalization in the first 2 years of life
Authors: Basso Olga , Shapiro Gabriel D , Twardowski Sarah E , Monnier Patricia , Buckett William , Tamblyn Robyn 
Published: July 2022

Abstract

STUDY QUESTION
Do publicly funded fertility treatment and single embryo transfer (SET) result in lower hospitalization rates of children of parents with infertility?

SUMMARY ANSWER
Following the 2010 Quebec law introducing free fertility treatment and SET, neonatal intensive care unit (NICU) admissions decreased among all children born to parents with infertility, but not among singletons, whose risk remained slightly higher than that of children of parents without infertility, even accounting for treatment and maternal age.

WHAT IS KNOWN ALREADY
Previous studies reported lower NICU admission rates among children conceived with ART after the 2010 law; however, children conceived without ART by parents with infertility were not considered.

STUDY DESIGN, SIZE, DURATION
Cohort study of children born in 1997–2017 to patients evaluated for infertility (‘exposed’) at an academic fertility center in Montreal (Canada) in 1996–2015. A random sample of births to Montreal residents served as comparison. Outcomes were identified from Quebec administrative databases.

PARTICIPANTS/MATERIALS, SETTING, METHODS
We compared children’s healthcare utilization before and after the 2010 law in 6273 exposed and 12 583 randomly sampled births (6846 and 12 775 children, respectively). We repeated the analysis among children conceived in the 63 months before and after the law (‘restricted period’), and examined whether differences in twinning, fertility treatment, and maternal age explained the higher risk of NICU admission among children of parents with infertility.

MAIN RESULTS AND THE ROLE OF CHANCE
In the exposed cohort, the proportion of twin births and of several adverse outcomes declined after the law. NICU admission and duration of NICU stay decreased overall, but not in singletons. Both measures remained higher in exposed children. Except for NICU admission, hospitalization rates were similar in exposed and random sample children. After accounting for fertility treatment and maternal age, exposed singletons were 17% more likely to be admitted to the NICU than children of parents with no medical history of infertility.

LIMITATIONS, REASONS FOR CAUTION
Sample size was relatively small; infertile patients were from a single center and the random sample from one city. Despite some limitations, administrative databases are likely to accurately reflect healthcare utilization.

WIDER IMPLICATIONS OF THE FINDINGS
Universal access to treatment and, particularly, SET results in an overall reduction of adverse outcomes among children conceived with treatment; however, children of parents with infertility are at a slightly higher risk, regardless of treatment.

STUDY FUNDING/COMPETING INTEREST(S)
This study was funded by the Canadian Institutes for Health Research (CIHR, grant no. 123362). No competing interests.

TRIAL REGISTRATION NUMBER
N/A.
Two unusual variants of pulmonary intra-lobar sequestration
Authors: Pauels Laura , De Waele Michèle , Medart Laurent , Debruche Mathieu 
Published: July 2022

Abstract
A pulmonary sequestration is a congenital malformation characterized by non-functional lung tissue with abnormal arterial systemic supply and abnormal connection to the bronchial tree. This may lead to recurrent infections rendering a surgical intervention more demanding. Because of multiple anatomic variations, it is important to obtain high-quality preoperative radiological clarification to determine the most suitable surgical approach. Although a non-surgical technique, consisting of embolization of the aberrant artery has been described, a surgical technique remains the treatment of choice in operable patients. Preoperative embolization of the aberrant artery may reduce the risk of haemorrhage but could cause technical challenges in a hybrid approach and therefore unforeseen peroperative stress to the surgical team. We report 2 adult patients with unusual intra-lobar sequestration with aberrant vascular rare anatomy. Both were treated by surgery. In the latter patient, we performed a hybrid approach. This was complicated by peroperative coils exposure making it a technical challenge to proceed.
Tipping Points: Challenges in Analyzing International Crisis Escalation
Authors: Chen Chong , Roberts Jordan , Adhikari Shikshya , Asal Victor , Beardsley Kyle , Gonzalez Edward , Jahanbani Nakissa , James Patrick , Lobell Steven E , Ripsman Norrin M , Silverstone Scott , van Wijk Anne 
Published: July 2022

Abstract
Why do some near crises tip over into full-blown crisis and others do not? This paper considers existing scholarship and identifies four key barriers to using quantitative analysis for tipping-point analyses: strategic indeterminacy; the incentives for conflict parties to avoid inefficiencies; the paucity of cases; and the availability of quality data. Due to these challenges, many do not perform well as immediate causes for crisis escalation. We also argue and demonstrate through two quantitative models of crisis escalation that some variables, particularly related to domestic politics, can do well in explaining why some disputes tip into crisis and others do not. As we illustrate with reference to the 1995–1996 Third Taiwan Straits Crisis, qualitative approaches that analyze the processes by which leaders and foreign policy institutions make decisions add needed explanatory power to purely quantitative models of the potential for near crises to tip into crisis.
Conventional papillary thyroid carcinoma with intraglandular lymphatic dissemination shows more aggressive features
Authors: Lei Yuanyuan , Feng Sha , Yu Qiuxiao , Shen Guihua , Yuan Lijuan , Huang Wenting 
Published: July 2022

Abstract

Objective
To investigate the invasive capability and other clinicopathological features of conventional papillary thyroid carcinoma (CVPTC) with intraglandular lymphatic dissemination.

Methods
Seventy-three conventional papillary thyroid carcinoma patients receiving total thyroidectomy were analyzed in this study. The expression of BRAF-V600E, D2-40 and CD31 in all thyroid samples was detected by immunohistochemical staining. The results were evaluated by two pathologists and were statistically analyzed. The rate of positive BRAF-V600E expression and the clinical invasiveness of CVPTC with intraglandular dissemination, multifocal non-intraglandular dissemination-CVPTC and single focus-CVPTC were evaluated. The correlation between BRAF-V600E expression, lymphatic vessel density, microvessel density and the clinicopathological characteristics of conventional papillary thyroid carcinoma were assessed.

Results
Twenty-five intraglandular dissemination-CVPTC, 17 multifocal non-intraglandular dissemination-CVPTC and 31 single focus-CVPTC cases were included in this study. The results showed that BRAF-V600E expression was independently correlated with intraglandular dissemination, age and pN staging (P &lt; 0.05). The lymphatic vessel density in the intraglandular dissemination-CVPTC group was higher than that in the non-intraglandular dissemination-CVPTC group (P &lt; 0.05). Compared with cases without intraglandular dissemination, intraglandular dissemination-CVPTC was associated with a younger age, higher lymph node metastasis rate, pN staging, the expression of BRAF-V600E and increased Capsule invasion and lymphovascular tumor thrombus (P &lt; 0.05). During the follow-up of 30 months (median 15 months), two patients in the intraglandular dissemination-CVPTC group had cervical lymph node metastasis after the first operation.

Conclusions
Intraglandular dissemination-CVPTC shows more aggressive features, and intraglandular lymphatic dissemination may be a potential biological indicator of poor prognosis.
Structures of multisubunit membrane complexes with the CRYO ARM 200
Authors: Gerle Christoph , Kishikawa Jun-ichi , Yamaguchi Tomoko , Nakanishi Atsuko , Çoruh Orkun , Makino Fumiaki , Miyata Tomoko , Kawamoto Akihiro , Yokoyama Ken , Namba Keiichi , Kurisu Genji , Kato Takayuki 
Published: July 2022

Abstract
Progress in structural membrane biology has been significantly accelerated by the ongoing ‘Resolution Revolution’ in cryo-electron microscopy (cryo-EM). In particular, structure determination by single-particle analysis has evolved into the most powerful method for atomic model building of multisubunit membrane protein complexes. This has created an ever-increasing demand in cryo-EM machine time, which to satisfy is in need of new and affordable cryo-electron microscopes. Here, we review our experience in using the JEOL CRYO ARM 200 prototype for the structure determination by single-particle analysis of three different multisubunit membrane complexes: the Thermus thermophilus V-type ATPase VO complex, the Thermosynechococcus elongatus photosystem I monomer and the flagellar motor lipopolysaccharide peptidoglycan ring (LP ring) from Salmonella enterica.
Compartmentalization, a key mechanism controlling the multitasking role of the SnRK1 complex
Authors: Gutierrez-Beltran Emilio , Crespo Jose L 
Published: July 2022

Abstract
SNF1-related protein kinase 1 (SnRK1), the plant ortholog of mammalian AMP-activated protein kinase/fungal (yeast) Sucrose Non-Fermenting 1 (AMPK/SNF1), plays a central role in metabolic responses to reduced energy levels in response to nutritional and environmental stresses. SnRK1 functions as a heterotrimeric complex composed of a catalytic α- and regulatory β- and βγ-subunits. SnRK1 is a multitasking protein involved in regulating various cellular functions, including growth, autophagy, stress response, stomatal development, pollen maturation, hormone signaling, and gene expression. However, little is known about the mechanism whereby SnRK1 ensures differential execution of downstream functions. Compartmentalization has been recently proposed as a new key mechanism for regulating SnRK1 signaling in response to stimuli. In this review, we discuss the multitasking role of SnRK1 signaling associated with different subcellular compartments.
KMT-2021-BLG-0171Lb and KMT-2021-BLG-1689Lb: two microlensing planets in the KMTNet high-cadence fields with followup observations
Authors: Yang Hongjing , Zang Weicheng , Gould Andrew , Yee Jennifer C , Hwang Kyu-Ha , Christie Grant , Sumi Takahiro , Zhang Jiyuan , Mao Shude ,   (Leading Authors), Albrow Michael D , Chung Sun-Ju , Han Cheongho , Jung Youn Kil , Ryu Yoon-Hyun , Shin In-Gu , Shvartzvald Yossi , Cha Sang-Mok , Kim Dong-Jin , Kim Hyoun-Woo , Kim Seung-Lee , Lee Chung-Uk , Lee Dong-Joo , Lee Yongseok , Park Byeong-Gon , Pogge Richard W ,   (The KMTNet Collaboration), Drummond John , Maoz Dan , McCormick Jennie , Natusch Tim , Penny Matthew T , Zhu Wei ,   (The MAP, μFUN Follow-up Teams), Bond Ian A , Abe Fumio , Barry Richard , Bennett David P , Bhattacharya Aparna , Donachie Martin , Fujii Hirosane , Fukui Akihiko , Hirao Yuki , Itow Yoshitaka , Kirikawa Rintaro , Kondo Iona , Koshimoto Naoki , Li Man Cheung Alex , Matsubara Yutaka , Muraki Yasushi , Miyazaki Shota , Olmschenk Greg , Ranc Clément , Rattenbury Nicholas J , Satoh Yuki , Shoji Hikaru , Silva Stela Ishitani , Suzuki Daisuke , Tanaka Yuzuru , Tristram Paul J , Yamawaki Tsubasa , Yonehara Atsunori ,   (The MOA Collaboration)
Published: July 2022

ABSTRACT
Follow-up observations of high-magnification gravitational microlensing events can fully exploit their intrinsic sensitivity to detect extrasolar planets, especially those with small mass ratios. To make followup observations more uniform and efficient, we develop a system, HighMagFinder, to automatically alert possible ongoing high-magnification events based on the real-time data from the Korea Microlensing Telescope Network (KMTNet). We started a new phase of follow-up observations with the help of HighMagFinder in 2021. Here we report the discovery of two planets in high-magnification microlensing events, KMT-2021-BLG-0171 and KMT-2021-BLG-1689, which were identified by the HighMagFinder. We find that both events suffer the ‘central-resonant’ caustic degeneracy. The planet-host mass-ratio is q ∼ 4.7 × 10−5 or q ∼ 2.2 × 10−5 for KMT-2021-BLG-0171, and q ∼ 2.5 × 10−4 or q ∼ 1.8 × 10−4 for KMT-2021-BLG-1689. Together with two other events, four cases that suffer such degeneracy have been discovered in the 2021 season alone, indicating that the degenerate solutions may have been missed in some previous studies. We also propose a quantitative factor to weight the probability of each solution from the phase space. The resonant interpretations for the two events are disfavoured under this consideration. This factor can be included in future statistical studies to weight degenerate solutions.
Uncovering Signals of Positive Selection in Peruvian Populations from Three Ecological Regions
Authors: Caro-Consuegra Rocio , Nieves-Colón Maria A , Rawls Erin , Rubin-de-Celis Verónica , Lizárraga Beatriz , Vidaurre Tatiana , Sandoval Karla , Fejerman Laura , Stone Anne C , Moreno-Estrada Andrés , Bosch Elena 
Published: July 2022

AbstractPeru hosts extremely diverse ecosystems which can be broadly classified into the following three major ecoregions: the Pacific desert coast, the Andean highlands, and the Amazon rainforest. Since its initial peopling approximately 12,000 years ago, the populations inhabiting such ecoregions might have differentially adapted to their contrasting environmental pressures. Previous studies have described several candidate genes underlying adaptation to hypobaric hypoxia among Andean highlanders. However, the adaptive genetic diversity of coastal and rainforest populations has been less studied. Here, we gathered genome-wide single-nucleotide polymorphism-array data from 286 Peruvians living across the three ecoregions and analyzed signals of recent positive selection through population differentiation and haplotype-based selection scans. Among highland populations, we identify candidate genes related to cardiovascular function (TLL1, DUSP27, TBX5, PLXNA4, SGCD), to the Hypoxia-Inducible Factor pathway (TGFA, APIP), to skin pigmentation (MITF), as well as to glucose (GLIS3) and glycogen metabolism (PPP1R3C, GANC). In contrast, most signatures of adaptation in coastal and rainforest populations comprise candidate genes related to the immune system (including SIGLEC8, TRIM21, CD44, and ICAM1 in the coast; CBLB and PRDM1 in the rainforest; and BRD2, HLA-DOA, HLA-DPA1 regions in both), possibly as a result of strong pathogen-driven selection. This study identifies candidate genes related to human adaptation to the diverse environments of South America.
COVID-19 in familial Mediterranean fever: Clinical course and complications related to primary disease
Authors: Avanoglu Guler Aslihan , Yuce Inel Tuba , Kasifoglu Timucin , Coskun Cansu , Karadeniz Hazan , Yildirim Derya , Bilici Reyhan , Satis Hasan , Kucuk Hamit , Haznedaroglu Seminur , Goker Berna , Ozturk Mehmet Akif , Sari Ismail , Tufan Abdurrahman 
Published: July 2022

ABSTRACT

Objectives
To evaluate the impact of familial Mediterranean fever (FMF) features on the clinical course and outcomes of coronavirus disease 2019 (COVID-19) and clinical course of FMF after COVID-19.

Methods
Consecutive FMF patients with COVID-19 were enrolled from three referral hospitals. Clinical features of FMF and detailed COVID-19 information were obtained from patient interviews and medical records.

Results
Seventy-three FMF patients were included in the study. 94.5% of patients had clinical symptoms of COVID-19. We found 24.7% hospitalization, 12.3% respiratory support, 4.1% intensive care unit admission, 6.8% complication, and 1.4% mortality rate in patients. The risk factors of hospitalization for respiratory support were male gender [OR: 7.167 (95% CI: 1.368–37.535)], greater age [OR: 1.067 (95% CI: 1.016–1.121)], and non-adherence to colchicine treatment before the infection [OR: 7.5 (95% CI: 1.348–41.722)]. One-third of patients had reported attacks after COVID-19. The patterns of triggered attacks were fever, peritonitis, pleuritis, transient arthritis, chronic knee mono-arthritis, and protracted febrile myalgia.

Conclusions
FMF characteristics were not associated with worse outcomes of COVID-19. Colchicine non-adherence was the risk factor of hospitalization for oxygen support. The rate of FMF attacks after COVID-19 is prominently increased, with some of them being protracted and destructive.
Optical and near-infrared photometric and polarimetric monitoring at flaring state of BL Lacertae in 2020–2021
Authors: Hazama Natsuko , Sasada Mahito , Imazawa Ryo , Fukazawa Yasushi , Kawabata Koji S , Nakaoka Tatsuya , Akitaya Hiroshi 
Published: July 2022

Abstract
BL Lacertae (BL Lac) was in an unprecedented bright state since 2020 August. We have conducted long-term photometric and polarimetric observations of BL Lac in optical and near-infrared (NIR) bands using the Kanata telescope in the Higashi-Hiroshima Observatory. BL Lac had simultaneously brightened in the NIR, optical, X-ray, and gamma-ray bands during the brightening period. There were two peaks of brightness in the optical and NIR bands. The degree of polarization and electric-vector position angle (EVPA) were also found to have fluctuated in the optical and NIR bands. A bluer-when-brighter trend was observed between the optical and NIR bands during the entire period of our observation. Furthermore, during the first bright period, the EVPA became parallel to the position angle of the jet. On the other hand, the polarization during the second brightening showed a different behavior from that during the first brightening. The brightenings can be explained by a combination of a shock acceleration in which a shock was generated with the perpendicular-direction shock front of the jet and a variation of the inclination angle.
Beneficial BacteriumAzospirillum brasilenseInduces Morphological, Physiological and Molecular Adaptation to Phosphorus Deficiency inArabidopsis
Authors: Sun Nan , Huang Lin , Zhao Hongcheng , Zhang Nan , Lin Xianyong , Sun Chengliang 
Published: July 2022

AbstractAlthough most cultivated soils have high levels of total phosphorus (P), the levels of bioavailable inorganic P (Pi) are insufficient. The application of plant-growth-promoting rhizobacteria (PGPR) is an eco-friendly strategy for P utilization; however, PGPR-mediated plant responses that enhance Pi acquisition remain unexplored. Here, we investigated the effect of Azospirillum brasilense on Arabidopsis adaptation to Pi deficiency. Results showed that A. brasilense inoculation alleviated Pi-deficiency-induced growth inhibition and anthocyanin accumulation and increased the total P content in Arabidopsis plants. A comprehensive analysis of root morphology revealed that A. brasilense increased root hair density and length under Pi-limited conditions. We further demonstrated that A. brasilense enhanced the acid phosphatase activity and upregulated the expression of several Pi transporter genes, such as PHOSPHATE1 (PHO1), PHOSPHATE TRANSPORTER 1:(PHT1:1) and PHT1;4. However, A. brasilense did not enhance the growth o total P content in pht1;1, pht1;4 and pht1;1pht1;4 mutants. Moreover, A. brasilense could not increase the P content and PHT1;1 expression in the root hairless mutant rsl4rsl2, because of the occurrence of low-Pi-induced PHT1;1 and PHT1;4 in root hairs. These results indicate that A. brasilense can promote root hair development and enhance acid phosphatase activity and Pi transporter expression levels, consequently improving the Pi absorption capacity and conferring plant tolerance to Pi deficiency.
A Role for Allantoate Amidohydrolase (AtAAH) in the Germination of Arabidopsis thaliana Seeds
Authors: Yazdanpanah Farzaneh , Willems Leo A J , He Hanzi , Hilhorst Henk W M , Bentsink Leónie 
Published: July 2022

Abstract
Seed dormancy is a very complex trait controlled by interactions between genetic and environmental factors. Nitrate is inversely correlated with seed dormancy in Arabidopsis. This is explained by the fact that seed dry storage (after-ripening) reduces the need for nitrogen for germination. When nitrate is absorbed by plants, it is first reduced to nitrite and then to ammonium for incorporation into amino acids, nucleic acids and chlorophyll. Previously, we showed that ALLANTOATE AMIDOHYDROLASE (AtAAH) transcripts are up-regulated in imbibed dormant seeds compared with after-ripened seeds. AAH is an enzyme in the uric acid catabolic pathway which catalyzes the hydrolysis of allantoate to yield CO2, NH3 and S-ureidoglycine. This pathway is the final stage of purine catabolism, and functions in plants and some bacteria to provide nitrogen, particularly when other nitrogen sources are depleted. Ataah mutant seeds are more dormant and accumulate high levels of allantoate, allantoin and urea, whereas energy-related metabolites and several amino acids are lower upon seed imbibition in comparison with Columbia-0. AtAAH expression could be detected during the early stages of seed development, with a transient increase around 8 d after pollination. AtAAH expression is the highest in mature pollen. The application of exogenous potassium nitrate can partly complement the higher dormancy phenotype of the Ataah mutant seeds, whereas other nitrogen sources cannot. Our results indicate that potassium nitrate does not specifically overcome the alleviated dormancy levels in Ataah mutant seeds, but promotes germination in general. Possible pathways by which AtAAH affects seed germination are discussed.
Communication network within the essential AAA-ATPase Rix7 drives ribosome assembly
Authors: Kocaman Seda , Lo Yu-Hua , Krahn Juno M , Sobhany Mack , Dandey Venkata P , Petrovich Matthew L , Etigunta Suhas K , Williams Jason G , Deterding Leesa J , Borgnia Mario J , Stanley Robin E 
Published: July 2022

Abstract
Rix7 is an essential AAA+ ATPase that functions during the early stages of ribosome biogenesis. Rix7 is composed of three domains including an N-terminal domain (NTD) and two AAA+ domains (D1 and D2) that assemble into an asymmetric stacked hexamer. It was recently established that Rix7 is a presumed protein translocase that removes substrates from preribosomes by translocating them through its central pore. However, how the different domains of Rix7 coordinate their activities within the overall hexameric structure was unknown. We captured cryo-electron microscopy (EM) structures of single and double Walker B variants of full length Rix7. The disordered NTD was not visible in the cryo-EM reconstructions, but cross-linking mass spectrometry revealed that the NTD can associate with the central channel in vitro. Deletion of the disordered NTD enabled us to obtain a structure of the Rix7 hexamer to 2.9 Å resolution, providing high resolution details of critical motifs involved in substrate translocation and interdomain communication. This structure coupled with cell-based assays established that the linker connecting the D1 and D2 domains as well as the pore loops lining the central channel are essential for formation of the large ribosomal subunit. Together, our work shows that Rix7 utilizes a complex communication network to drive ribosome biogenesis.