bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beachrocks of the last low sea level, substrate of the barrier reef system along the outer Guiana shelf
Authors: Giresse Pierre , Loncke Lies , Heuret Arnauld , Longueville François , Casanova Alexandre , Sadaoui Mahrez 
Published: September 2022

Abstract

The Great Amazon Reef System is a living biogenic mesophotic reef ecosystem that has been recently described along the shelf break of Brazil. An exploration mission was carried out in 2019 along the outer edge of the French Guiana Shelf. A side-scan sonar survey was conducted to locate reef outcrops and allowed twelve in situ 80 to 120 m depth dives and sampling of the reef rocks and peripheral sands. The majority of the hard-rocks are composed of biological concretions. However several fragments revealed the inside presence of sandstone clasts. These clasts, more or less enveloped by biogenic coatings, probably represent destroyed clasts of underlying or neighboring beachrock banks. Their dominant cement is micritic (high-magnesian calcite); the intergranular or extragranular porous field was later filled with low-magnesian sparry calcite. The sand or gravel that accumulated near the barrier mainly consist of the blunt debris of coastal fauna and flora associated with different carbonate or ferruginous neoformed ooids. At 104 m depth, ooids extracted from Dive 11 samples dated from the start of MIS2 (27,370 cal yr BP) and attest to the presence of a significant coastal accumulation. At this same site, cementing did not take place until about 3,500 years later (23,990 cal yr BP). The cement of a nearby beachrock indicates a much more recent age (16,170 cal yr BP). Lastly, the age of 4,100 year BP measured on the barnacles attached to the top of the reef attests to the late Holocene reef's biological activity.
TIMP3 Gene Upstream CpG Island Methylation is Associated with BRAF V600E Mutation in Thyroid Neoplasms
Authors: Zarkesh Maryam , Arab Noman , Heydarzadeh Shabnam , Nozhat Zahra , Abooshahab Raziyeh , Jahromi Marziyeh Salehi , Fanaei Seyed Ahmad , Hedayati Mehdi 
Published: September 2022

Abstract
Aims:
Extensive evidence demonstrated that epigenetic modifications play a crucial role in the development of thyroid malignancies. The onset of DNA methylation sequencing technologies has provided a more comprehensive understanding of biological development of tumorigenesis. This cross-sectional study aimed to investigate the methylation degree of a large region of tissue inhibitor of metalloproteinase 3 (TIMP3) promoter, including 15 CpG islands, and its association with BRAF V600E mutation in thyroid nodules.
Methods
Thyroid specimens from 64 patients met the eligibility criteria, consisting of 28 PTC, 9 FTC, and 27 benign subjects. Exon 15 of the BRAF gene was genotyped using sequencing. The expression of TIMP3 was evaluated using the real-time qRT-PCR method. The bisulfite sequencing PCR technique was used to estimate the promoter methylation status.
Results
The BRAF V600E mutation was found in 46.2% of PTC and none of FTC cases. TIMP3 mRNA level was reduced in both PTC and FTC tumoral tissues compared to the adjacent non-tumoral tissues (ANT) (p = 0.02 and p = 0.03, respectively). Moreover, a reduction of TIMP3 expression was observed in FTC tissues compared to the benign samples (p = 0.04). It is noteworthy that significant hypermethylation was found in TIMP3 promoter in both PTC and FTC tissues compared to the ANT and benign samples (p < 0.05). The BRAF mutation was associated with TIMP3 expression and total methylation in PTC. There was also a significant correlation between the expression and total methylation status in ANT and tumoral tissues of PTC and FTC cases (p < 0.05).
Conclusions
Disturbance in TIMP3 promoter's methylation individually may be beneficial in differentiating benign and malignant thyroid tissues. According to the reversibility of DNA methylation, these events may be considered potential targets for demethylation treatments.
Study on impact damage defects of carbon fiber reinforced composites by optical pulse thermography
Authors: Liu Guozeng , Gao Weicheng , Liu Wei , Wei Yanze , Xu Jianxun , Li Rui , Shen Runhong 
Published: September 2022

Abstract
Carbon fiber reinforced composites (CFRP) are widely used in the aviation industry and other fields. During service, the surface of CFRP is vulnerable to impact and damage defects, which poses a threat to the safety of CFRP quality. The halogen lamp is used as the active heat source of the sample. Based on the built optical pulse thermography (OPT) test system, The sample with impact damage under long pulse excitation is studied. The thermal response of impact damage samples was collected by infrared camera A655sc. The original infrared image sequence is processed by pulse phase method (PPT), thermal signal reconstruction (TSR), and total harmonic distortion (THD). The results show that the signal-to-noise ratio (SNR) of Original images processed by PPT, TSR, and THD is improved, while the SNR of THD is significantly improved. More impact damage defects recognition effect is obtained by optical pulse thermography.
Platelets-derived vesicles: innovative subcellular platforms for management of multiple sclerosis
Authors: mehdi-alamdarlou Sanaz , Ahmadi Fatemeh , Shahbazi Mohammad-Ali , Azadi Amir , ashrafi hajar 
Published: September 2022

Abstract
Multiple sclerosis (MS) is a progressive inflammatory autoimmune disease that involves young individuals. The drug delivery systems now are available for this disease have chronic and non-targeted effects in the patients, and because of the presence of BBB, their concentration in the CNS is low. Because of this flaw, it is critical to use innovative active targeted drug delivery methods. Platelets are blood cells that circulate freely and play an important role in blood hemostasis. In this review, we emphasize the various roles of activated platelets in the inflammatory condition to recruit other cells to the injured area and limit the inflammation. Besides, the activated platelets in the different stages of the MS disease play a significant role for limiting the progression of inflammation in the peripheral area and CNS. This evidence indicates that platelet-based drug delivery system can be an efficient candidate for drug targeting to the CNS and limiting the inflammation in the peripheral and central areas for MS therapy.
PKR-like ER kinase (PERK) Haplotypes Are Associated with Depressive Symptoms in People with HIV
Authors: Haddadi Sana , Jordan-Sciutto Kelly L. , Akay-Espinoza Cagla , Grelotti David , Letendre Scott L. , Tang Bin , Ellis Ronald J. 
Published: September 2022

Abstract
Background. Depression is a debilitating and difficult-to-treat condition in people with HIV (PWH) despite viral suppression on antiretroviral therapy (ART). Depression is associated with activation of the PKR-like ER kinase (PERK) pathway, which regulates protein synthesis in response to metabolic stress. We evaluated common PERK haplotypes that influence PERK expression in relation to depressed mood in PWH.
Methods. PWH from 6 research centers were enrolled in the study. Genotyping was conducted using targeted sequencing with TaqMan. The major PERK haplotypes A, B, and D were identified. Depressive symptom severity was assessed using the Beck Depression Inventory-II (BDI-II). Covariates including genetically-defined ancestry, demographics, HIV disease/treatment parameters and antidepressant treatments were assessed. Data were analyzed using multivariable regression models.
Results. A total of 287 PWH with a mean (SD) age of 57.1±7.8 years were enrolled. Although the largest ethnic group was non-Hispanic white (n=129, 45.3%), African-American (n=124, 43.5%) and Hispanic (n=30, 10.5%) made up over half the sample. 20.3% were female and 96.5% were virally suppressed. Mean BDI-II was 9.6±9.5, and 28.9% scored above the cutoff for mild depression (BDI-II>13). PERK haplotype frequencies were AA 57.8%, AB 25.8%, AD 10.1%, and BB 4.88%. PERK haplotypes were differentially represented according to genetic ancestry (p= 6.84e-6). BDI-II scores were significantly higher in participants with the AB haplotype (F=4.45, p=0.0007). This finding was robust to consideration of potential confounds.
Conclusions. PERK haplotypes were associated with depressed mood in PWH. Consequently, pharmacological targeting of PERK-related pathways might ameliorate depression in PWH.
An Analysis of the Knowledge of Adults Aged Between 18-45 on HPV along with their Attitudes and Beliefs about HPV Vaccine: The Cyprus Case
Authors: YARİCİ Filiz , MAMMADOV Betül 
Published: September 2022

Abstract
Background
The aim of this research is to analyze prior knowledge of adults between 18–45 years of age and living in the Northern Cyprus about Human Papilloma Virus (HPV) along with their attitudes and beliefs towards HPV vaccine.
Materials and Methods
The research, which was planned as a correlational survey, was executed on the web. The research was completed with 1108 adults between 18–45 years of age, living in the Northern Cyprus and volunteered to participate in the study.
Results
51.90% of the adults participating in the study were found to be women while 48.10% were men; 8.84% had a Sexually transmitted disease (STD) before and 63.27% of the individuals who had a sexually transmitted disease before also had had HPV and they knew it, 36.73% had had HPV before but did not know it; 77.55% had undergone a treatment for their disease whereas 22.45% did not receive any treatment; 59.18% were found to be actively infected with HPV. Statistically significant and positive correlations were determined between the overall scores of the participants from the Human Papillomavirus Knowledge Questionnaire (HPV-KQ) and their scores in the perceived severity, perceived benefits and perceived susceptibility sub-dimensions of the Health Belief Model Scale for Human Papilloma Virus and Its Vaccination (HBMS-HPVV) (p < 0.05). There was a statistically significant and negative correlation between HPV-KQ scores, questions on Current HPV Vaccination Program and the perceived barriers sub-dimension of the HBMS-HPVV whereas there was a statistically significant and positive correlation between the HPV-KQ scores, questions on Current HPV Vaccination Program and the perceived benefits and perceived susceptibility sub-dimensions of the HBMS-HPVV (p < 0.05). There was a statistically significant and positive correlation between HPV-KQ scores and the perceived severity, perceived benefits and perceived susceptibility sub-dimensions of the HBMS-HPVV whereas there was a statistically significant and negative correlation between HPV-KQ scores and the perceived barriers sub-dimension of the HBMS-HPVV (p < 0.05).
Conclusions
Trainings should be planned to raise the awareness of individuals and cause a behavioral change on the protection from HPV and HPV vaccines; brochures and social media awareness videos should be prepared and health care policies such as free vaccination should be developed.
Early-life exposure to multiple micronutrient deficiencies induces clinically-relevant antibiotic resistance in a pre-clinical murine model
Authors: Littlejohn Paula , Metcalfe-Roach Avril , Holani Ravi , Bar-Yoseph Haggai , Fan Yiyun , Finlay B 
Published: September 2022

Abstract
Globally, ~340 million children (<5 years) suffer from multiple micronutrient deficiencies, accompanied by high pathogenic burden and death due to multi-drug resistant bacteria. The role of early-life micronutrient deficiencies in mitigating antibiotic resistance within the host microbiota remains unexplored. We developed a postnatal preclinical murine model deficient in multiple micronutrients: zinc, folate, iron, vitamin A, and vitamin B12. Fecal metagenomic sequencing after dietary exposure revealed enrichment in the relative abundance of pathobionts and antibiotic resistance mechanisms including efflux pumps, target drug site alteration, antibiotic degrading enzymes, permeability, and acquisition of antibiotic resistance genes (ARGs). Despite being antibiotic naïve, we observed increased abundance of genes encoding resistance to several antibiotic classes: tetracycline, vancomycin, methicillin, fosfomycin, aminoglycosides and glycopeptides. Moreover, in vitro antibiotic susceptibility testing revealed clinically-relevant resistance to multiple antibiotic classes, namely vancomycin, methicillin, amoxicillin and erythromycin in mice fed the low-micronutrient diet. These novel findings present an unappreciated and profound role of cooccurring multiple micronutrient deficiencies in contributing to the global burden of antibiotic resistance, with significant clinical implications for undernourished children living in low-to-mid income countries.
Performance Optimization of Intelligent- Reflecting-Surfaces assisted-FSO link
Authors: Singh Deepak Kumar , Tiwari B B 
Published: September 2022

Abstract
Free space optics (FSO) plays key role to establish a link in targeted remote and uncovered area of skip-zones where wireline (optical fiber cable) is not easy to deploy. FSO communication has been opted as a promising solution for reliable, low latency, cost effective and unlicensed spectrum wireless backhaul connectivity for 5G and beyond wireless communication system. The foremost impairments of FSO communication are atmospheric turbulence and line-of-sight (LoS) requirement between Source to Destination. To relax the LoS requirement of FSO communication, a novel scheme called intelligent reflecting surface (IRS)-assisted FSO communication is a very reliable and effective alternative solution like first-last mile access connectivity. In this work, an IRS-based FSO and radio frequency (RF) links are modeled. IRS-assisted FSO link achieves average symbol error-rate (ASER) of 10− 6 for SNR of 30 dB while FSO link with Gamma-Gamma and Malaga (M) distribution gives ASER value of 10− 3 and 10− 2 respectively. In Optical-multiple IRS-assisted FSO links, increase in number of reflecting elements improves ASER upto 10− 8 at SNR of 20dB.
SEAC: Serbian Emotional Amateur Cellphone Speech Corpus
Authors: Suzić Siniša , Nosek Tijana , Sečujski Milan , Popović Branislav , Krstanović Lidija , Vujović Mia , Simić Nikola , Janev Marko , Jakovljević Nikša , Delić Vlado 
Published: September 2022

Abstract

Emotional speech recognition and synthesis of expressive speech are highly dependable on the availability of emotional speech corpora. In this paper, we present the creation and verification of the Serbian Emotional Amateur Cellphone Speech Corpus (SEAC), which was released by the University of Novi Sad, Faculty of Technical Sciences in 2022, as the first amateur emotional speech corpus in Serbian language, recorded over cellphones. The corpus contains emotional speech elicited from 53 different speakers (24 male and 29 female) in 5 different emotional states (neutral, happiness, sadness, fear and anger), and its total duration amounts to approximately 8 hours of speech data. Initial objective evaluation of the corpus has confirmed high correlation between the behaviour of acoustic parameters corresponding to different emotional states in the newly recorded corpus and the existing Serbian emotional speech corpus recorded by 6 professional actors, which was used as a source for reference recordings. The corpus was further evaluated through listening tests concerned with human emotion recognition. Finally, we present the results of experiments concerning emotion recognition and speaker recognition by several conventional machine learning systems carried out on the corpus, as well as the results of a cross-lingual emotion recognition experiment involving a state-of-the-art machine learning system based on deep convolutional neural networks.
Efficacy and Safety of Corticosteroids in Cardiac Arrest: A Systematic Review, Meta- Analysis and Trial Sequential Analysis of Randomized Control Trials
Authors: Penn Jeremy , Douglas Will , Curran Jeffrey , Chaudhurix Dipayan , Dionne Joanna C. , Fernando Shannon , Granton David , Mathew Rebecca , Rochwerg Bram 
Published: September 2022

Abstract

Background
Post cardiac arrest, outcomes for most patients are poor, regardless of setting. Many patients who do achieve spontaneous return of circulation require vasopressor therapy to maintain organ perfusion. There is some evidence to support the use of corticosteroids in cardiac arrest.
Research Question:
Assess the efficacy and safety of corticosteroids in patients following in and out of hospital cardiac arrest.
Study Design & Methods:
We searched databases CINAHL, EMBASE, LILACS, MEDLINE, Web of Science, CENTRAL, ClinicalTrails.gov, and ICTRP. We included randomized controlled trials (RCTs) that examined the efficacy and safety of corticosteroids, as compared to placebo or usual care in patients post cardiac arrest. We pooled estimates of effect size using random effects meta-analysis and report relative risk (RR) with 95% confidence intervals (CIs). We assessed risk of bias (ROB) for the included trials using the modified Cochrane ROB tool and rated the certainty of evidence using Grading of Recommendations Assessment, Development and Evaluation methodology (GRADE).
Results
We included 8 RCTs (n = 2,213 patients). Corticosteroids administered post cardiac arrest had an uncertain effect on mortality measured at the longest point of follow-up (RR 0.96, 95% CI 0.90 to 1.02, very low certainty, required information size not met using trial sequential analysis). Corticosteroids probably increase return of spontaneous circulation (ROSC) (RR 1.32, 95% CI 1.18 to 1.47, moderate certainty) and may increase the likelihood of survival with good functional outcome (RR 1.49, 95% CI 0.87 to 2.54, low certainty). Corticosteroids may increase the risk of ventilator associated pneumonia (RR 0.76, 95% CI 0.46 to 1.09, low certainty) and renal failure (RR 1.29, 95% CI 0.84 to 1.99, low certainty) with an uncertain effect on bleeding (RR 2.04, 95% CI 0.53 to 7.84, very low certainty) and peritonitis (RR 10.54, 95% CI 2.99 to 37.19, very low certainty).
Conclusions
In patients post cardiac arrest, corticosteroids have an uncertain effect on mortality but may increase ROSC and the likelihood of survival with good functional outcome. The effect on complications is based on low or very low certainty evidence.
Identification of cuproptosis related genes in diagnosis and subtype classification of ALS using the Gene Expression Omnibus Database
Authors: Yang Aoran , Wang Xinhuan , Shang Chao , Hu Yaofeng , Yu Chenxue , Zhang Jian , Hong Yang 
Published: September 2022

Abstract
Cuproptosis is a newly discovered way of cell death which contributed to the accumulation of copper as well as targeting lipoylated TCA cycle proteins and what role that cuproptosis plays in ALS is still unknown. We analyzed 10 cuproptosis-related genes between ALS patients (233 samples) and non-ALS patients (508 samples) based on Gene Expression Omnibus (GEO) GSE112676. We constructed RF model to predict occurrence of ALS. By establishing coppercluster and gene cluster, we explored cuproptosis functioning patterns and immune cells infiltration in ALS and quantified these functioning patterns by erecting copperscore criterion. LIPT1, DLAT, DLD and PDHB were identified as differential expressed genes in ALS which high expression of them relates to pathogenicity of ALS. Moreover, T cell family, B cell family and dendritic family may highly be involved in the happening of ALS while mononuclear phagocyte system and nature killer cell family were silenced. Our findings provide potential immunotherapy and biomarkers to foresee the happening of ALS.
IL-10R Inhibition Reprograms Tumor-Associated Macrophages and
Reverses Drug Resistance in Multiple Myeloma
Authors: Sun Jennifer , Muz Barbara , Miari Katerina , Alhallak Kinan , Park Chaelee , Maksimos Mina , Lubben Berit , Chen Yixuan , Adebayo Ola , Bash Hannah , Kelly Sarah , Fiala Mark , Williams Mark , Bender Diane , Shokeen Monica , Vij Ravi , Azab Abdel Kareem 
Published: September 2022

Abstract

Multiple myeloma (MM) is the cancer of plasma cells within the bone marrow (BM) and remains incurable. Tumor-associated macrophages (TAMs) within the tumor microenvironment often display a pro-tumor phenotype and correlate with tumor proliferation, survival, and therapy resistance. Thus, TAMs have become an emerging target of interest. IL-10 is a key immunosuppressive cytokine that leads to recruitment and development of TAMs. In this study, we investigated the role of IL-10 in MM TAM development as well as the therapeutic application of IL-10/IL-10R signaling inhibition. We demonstrated that IL-10 is overexpressed in MM BM and mediates M2-like polarization of TAMs in patient BM, 3D co-cultures in vitro, and mouse models. In turn, TAMs promote MM proliferation and drug resistance, both in vitro and in vivo. Moreover, inhibition of IL-10/IL-10R pathway using a blocking IL-10R antibody prevented M2 polarization of TAMs and the consequent TAM-induced proliferation of MM, and re-sensitized MM to therapy, in vitro and in vivo. Therefore, our findings suggest that inhibition of IL-10/IL-10R axis is a novel immunotherapy strategy with monotherapy efficacy and can be further combined with current anti-MM therapy to overcome drug resistance. Future investigation is warranted to evaluate the potential of such therapy in MM patients.
The Impact of Using Dynamic Mathematics Learning Objects on Pre-Service Mathematics Teachers' Motivation and Cognitive Load Levels
Authors: ORAK Cihan , KÜÇÜK Sevda , GÜLCÜ Aslan 
Published: September 2022

Abstract
Computer-assisted mathematics education (CAME) continues to be the focus of interest for today's educators. The purpose of this study was to investigate the impact of teaching with dynamic mathematics learning objects (DMLO) in CAME on the motivation and cognitive load of pre-service mathematics teachers. The sample of the study, which used an explanatory design, one of the mixed research methods, consisted of 24 pre-service mathematics teachers. The participants constituted thesingle group pre-post-test, weak experimental design group. The experimental group participated in learning activities with DMLO prepared as part of CAME. In the research process, we used the Teaching Material Motivation Scale and the Cognitive Load Scale as quantitative data collection instruments, while we employed the semi-structured interview form as a qualitative data collection instrument. We analyzed quantitative data using the dependent group t-test and the qualitative data using the content analysis method. The results of the study showed that CAME using DMLO had a significant impact on motivation and cognitive load of pre-service teachers. It also showed that the images and graphics used with CAME were interesting, entertaining, and engaging. The results were analyzed along with the qualitative data obtained in accordance with the students' opinions and experiences, and the reasons for the quantitative results were explained. The current study is important in providing guidance on how DMLO technologies can be used more effectively in the learning process. In addition, the results obtained regarding the use of DMLO technologies in the learning process will be useful for providing different and up-to-date findings in the literature.