bluuebunny/crossref_metadata_2025_split huggingface dataset:
GPX4 Plays a Crucial Role in Fuzheng Kang’ai Decoction-Induced Non-Small Cell Lung Cancer Cell Ferroptosis
Authors: Zhao Yue-Yang , Yang Yu-Qi , Sheng Hong-Hao , Tang Qing , Han Ling , Wang Su-Mei , Wu Wan-Yin 
Published: April 2022

Background: Fuzheng Kang’ai decoction (FZKA) has been widely used to treat Non-Small Cell Lung Cancer (NSCLC) patients in China for decades, showing definitively curative effects in clinic. Recently, we found that FZKA could induce NSCLC cell ferroptosis, another type of programmed cell death (PCD), which is totally different from cell apoptosis. Therefore, in the present study, we aim to discover the exact mechanism by which FZKA induces NSCLC cell ferroptosis, which is rarely studied in Traditional Chinese Medicine (TCM).Methods: Cell proliferation assay were performed to detect the cell viability. Cell ferroptosis triggered by FZKA was observed by performing lipid peroxidation assay, Fe2+ Ions assay, and mitochondrial ultrastructure by transmission electron microscopy. Ferroptosis inhibitors including liproxstatin-1 and UAMC 3203 were used to block ferroptosis. The ratio of GSH/GSSG was done to measure the alteration of oxidative stress. Western blot and qRT-PCR were carried out to detect the expression of solute carrier family 7 member 11 (SLC7A11), solute carrier family 3 member 2 (SLC3A2) and glutathione peroxidase 4 (GPX4) at protein and mRNA levels, respectively. Lentivirus transfection was performed to overexpress GPX4 stably. Animal model was done to verify the effect of FZKA-induced ferroptosis in NSCLC in vivo and immunohistochemistry was done to detect the expression of SLC7A11, SLC3A2 and GPX4 at protein level.Results: First of all, in vitro experiments confirmed the inhibition effect of FZKA on NSCLC cell growth. We then, for the first time, found that FZKA induced NSCLC cell ferroptosis by increasing lipid peroxidation and cellular Fe2+ Ions. Moreover, characteristic morphological changes of NSCLC cell ferroptosis was observed under transmission electron microscopy. Mechanistically, GPX4, as a key inhibitor of lipid peroxidation, was greatly suppressed by FZKA treatment both at protein and mRNA levels. Furthermore, system xc− (SLC7A11 and SLC3A2) were found to be suppressed and a decreased GSH/GSSG ratio was observed at the same time when treated with FZKA. Notably, overexpressing GPX4 reversed the effect of FZKA-induced NSCLC cell ferroptosis significantly. Finally, the above effect was validated using animal model in vivo.Conclusion: Our findings conclude that GPX4 plays a crucial role in FZKA-induced NSCLC cell ferroptosis, providing a novel molecular mechanism by which FZKA treats NSCLC.
Acute Oxygen-Sensing via Mitochondria-Generated Temperature Transients in Rat Carotid Body Type I Cells
Authors: Rakoczy Ryan J. , Schiebrel Clay M. , Wyatt Christopher N. 
Published: April 2022

The Carotid Bodies (CB) are peripheral chemoreceptors that detect changes in arterial oxygenation and, via afferent inputs to the brainstem, correct the pattern of breathing to restore blood gas homeostasis. Herein, preliminary evidence is presented supporting a novel oxygen-sensing hypothesis which suggests CB Type I cell “hypoxic signaling” may in part be mediated by mitochondria-generated thermal transients in TASK-channel-containing microdomains. Distances were measured between antibody-labeled mitochondria and TASK-potassium channels in primary rat CB Type I cells. Sub-micron distance measurements (TASK-1: 0.33 ± 0.04 µm, n = 47 vs TASK-3: 0.32 ± 0.03 µm, n = 54) provided evidence for CB Type I cell oxygen-sensing microdomains. A temperature-sensitive dye (ERthermAC) indicated that inhibition of mitochondrial activity in isolated cells caused a rapid and reversible inhibition of mitochondrial thermogenesis and thus temperature in these microdomains. Whole-cell perforated-patch current-clamp electrophysiological recordings demonstrated sensitivity of resting membrane potential (Vm) to temperature: lowering bath temperature from 37°C to 24°C induced consistent and reversible depolarizations (Vm at 37°C: -48.4 ± 4.11 mV vs 24°C: -31.0 ± 5.69 mV; n = 5; p &lt; 0.01). These data suggest that hypoxic inhibition of mitochondrial thermogenesis may play an important role in oxygen chemotransduction in the CB. A reduction in temperature within cellular microdomains will inhibit plasma membrane ion channels, influence the balance of cellular phosphorylation–dephosphorylation, and may extend the half-life of reactive oxygen species. The characterization of a thermosensory chemotransduction mechanism, that may also be used by other oxygen-sensitive cell types and may impact multiple other chemotransduction mechanisms is critical if we are to fully understand how the CBs, and potentially other oxygen-sensitive cells, respond to hypoxia.
Resilience Among Parents of Adolescents With Type 1 Diabetes: Associated With Fewer Parental Depressive Symptoms and Better Pediatric Glycemic Control
Authors: Luo Dan , Wang Yubing , Cai Xue , Li Ruxue , Li Mingzi , Liu Haiyan , Xu Jingjing 
Published: April 2022

BackgroundAlthough pediatric resilience plays a significant role in resisting negative moods and improving glycaemic control, little research exists regarding resilience among the parents of adolescents with Type 1 diabetes.ObjectiveTo investigate parental resilience's correlations with parental depressive symptoms, parental diabetes distress, and pediatric glycaemic control.MethodsThis cross-sectional study recruited adolescents with Type 1 diabetes and their parents from two hospitals. The parents completed questionnaires. The 10-item Connor-Davidson Resilience Scale measured resilience; the Problem Areas in Diabetes Survey-Parent Revised version measured diabetes distress; the Patient Health Questionnaire-9 measured depressive symptoms. Standard glycated hemoglobin tests were performed on the adolescents.ResultsData from 224 parents (77.2% female, Mage = 39.88 [SD = 5.02], age range = 30–56 years) of adolescents (50.9% boys, Mage = 13.54 years [SD = 2.48], age range = 10–19 years) were available. More than half (52.7%) of parents exceeded the criterion score for high resilience. Parental resilience was significantly negatively associated with parental depressive symptoms and diabetes distress. Parents from the high-resilience group reported fewer depressive symptoms than those from the low-resilience group. In multivariate regressions, greater parental resilience is consistently related to better pediatric glycaemic control beyond parental psychological risk factors.ConclusionsThis study highlights the importance of parental resilience for parental mental health and glycaemic control among adolescents with Type 1 diabetes. The appropriate resilience support programme might be developed for parents, especially for those existing depressive symptoms and diabetes distress.
Horizontal Inequity in the Utilization of Maternal and Reproductive Health Services: Evidence From the 2018 Nigeria Demographic and Health Survey
Authors: Aregbeshola Bolaji Samson , Olaniyan Olanrewaju 
Published: April 2022

BackgroundMaternal mortality and poor reproductive health outcomes remain major public health challenges in low-resource countries. The Sustainable Development Goals have proposed new targets to reduce global maternal mortality ratio to 70 per 100,000 live births and ensure universal access to sexual and reproductive healthcare services by 2030. Inequity in the utilization of maternal and reproductive health services leads to poor reproductive health outcomes and maternal mortality. Despite reduction in global maternal mortality over the decades, the level of maternal mortality remains unacceptably high in Nigeria with limited attention given by governments to addressing health inequities. This study aimed to examine horizontal inequity in the utilization of maternal and reproductive health services in Nigeria.MethodsSecondary data from the 2018 Nigeria Demographic and Health Survey were utilized to examine horizontal inequity in the utilization of maternal and reproductive health services such as postnatal care, delivery by cesarean section, modern contraceptive use, and met need for family planning. Equity was measured using equity gaps, equity ratios, concentration curves, and concentration indices. All analyses were performed using ADePT 6.0 and STATA version 14.2 software.ResultsThe overall coverage level of postnatal care, delivery by cesarean section, modern contraceptive use, and met need for family planning was 20.81, 2.97, 10.23, and 84.22%, respectively. There is inequity in the utilization of postnatal care, delivery by cesarean section, and modern contraceptive favoring the rich, educated, and urban populations. Met need for family planning was found to be almost perfectly equitable.ConclusionThere is inequity in the utilization of maternal and reproductive health services in Nigeria. Inequity in the utilization of maternal and reproductive health services is driven by socioeconomic status, education, and location. Therefore, governments and policymakers should give due attention to addressing inequities in the utilization of maternal and reproductive health services by economically empowering women, improving their level of education, and designing rural health interventions. Addressing inequities in the utilization of maternal and reproductive health services would also be important toward achieving the Sustainable Development Goal targets 3.1 and 3.7.
Gap and Inequality in the Economic Income of Independent Workers in the Region of Puno-Peru and the Effect of the Pandemic, 2019–2020
Authors: Quispe Mamani Julio C. , Flores Turpo Giovana A. , Calcina Álvarez Dominga A. , Yapuchura Saico Cristóbal R. , Velásquez Velásquez Wily L. , Aguilar Pinto Santotomas L. , Quispe Quispe Betsy , Quispe Maquera Nelly B. , Cutipa Quilca Balbina E. 
Published: April 2022

ObjectiveThis article seeks to determine the social determinants of inequality in economic income in independent workers in the Puno region in the periods 2019 and 2020.MethodsFor which the quantitative approach was used, with descriptive and correlational design, considering the multiple regression model.ResultsIt was determined that there is a very significant income gap by educational level due to the productive differential that coronavirus disease 2019 (COVID-19) affected all the households; there is inequality in the economic income of independent workers, since in 2019, there was a greater inequality of economic income among independent workers (Gini = 0.6142) in relation to the national level (Gini = 0.415) and in 2020, the inequality of economic income increased due to COVID-19 problem, where the Gini coefficient amounted to 0.7136 in relation to the national level (Gini = 0.431).ConclusionThe determining factors of the economic income of the independent worker in the region of Puno in the periods 2019 and 2020 are the age that explains in 5.19 and 1.72%, the level of education that explains in 20.74 and 34.86% and the sex that explains in 37 and 14.19%, respectively.
Platelet-Rich Fibrin in Fat Grafts for Facial Lipofilling: A Randomized, Controlled Split-Face Clinical Trial
Authors: Zhang Zhao-Xiang , Qiu Li-Hong , Shi Nian , Xiong Shao-Heng , Ma Xian-Jie , Yi Cheng-Gang 
Published: April 2022

ObjectivePrevious studies have reported that platelet-rich fibrin (PRF) may enhance the efficacy of fat grafts in facial lipofilling. However, these studies either lacked objective data or were not randomized, controlled trials. Thus, we aimed to objectively evaluate the efficacy of PRF in facial lipofilling.MethodsA controlled, split-face, randomized trial (January 2018 to May 2019) based on 18 patients who underwent fat grafts for bilateral temple lipofilling was performed. Each patient received a combination of an autologous fat graft and PRF on one side and a fat graft combined with an equal volume of saline on the other side. The effects of PRF were evaluated by comparing the remaining bilateral fat graft volumes through a digital three-dimensional reconstruction technique. Improvements in the appearance and recovery time of each temple were assessed by both a surgeon and patients who were blinded to the treatment assignment. Complications were also recorded.ResultsBilateral temple lipofilling showed no evidence of fat embolism, vascular/nerve injury, infection, massive edema, or prolonged bruising. Three-dimensional reconstruction data and the assessments from both the surgeon and patients revealed no significant differences in fat graft retention volume between the PRF-positive and PRF-negative lipofilling groups. However, recovery time in the PRF-positive lipofilling sites was significantly shortened compared with that of the PRF-negative lipofilling sites.ConclusionFacial filling with autologous fat grafts is effective and safe. Our results show that PRF does not markedly improve fat graft volume retention in the temple but significantly reduces postoperative recovery time.Trial Registration NumberChiCTR2100053663.
Indications, Feasibility, and Safety of TST STARR Plus Stapler for Degree III Hemorrhoids: A Retrospective Study of 125 Hemorrhoids Patients
Authors: Wei Jun , Ding Xufeng , Jiang Jie , Ji Lijiang , Huang Hua 
Published: April 2022

BackgroundStapler hemorrhoidopexy (SH) has been widely accepted for hemorrhoids patients because of its low postoperative pain, but it is also associated with a high recurrence rate. The recurrence might be due to failure to completely remove the prolapsed tissue or insufficient removal capacity of the instruments. Removing more prolapsed tissue to reduce the recurrence is believed to benefit more severe prolapsed hemorrhoids patients.MethodsWe evaluated the short- and long-term safety and efficacy in 125 hemorrhoids patients who underwent SH in 2013–2015. Eighty patients had prolapsed tissue less than half of the circular anal dilator (CAD) and underwent a procedure for prolapsing hemorrhoids (PPH), while the remaining 45 patients with hemorrhoid prolapse greater than half of the CAD were treated with a tissue selection therapy stapler stapled transanal rectal resection plus (TST STARR+).ResultsThere were no significant differences between the two groups in terms of operative time, hospitalization time, overall satisfaction or complications. At follow-up of up to 4 years after surgery, there was no significant difference in recurrence rates between TST STARR+ group and PPH group (5.2% vs. 4.7%, p &lt; 0.05). The mean width and volume of the resected tissues were significantly larger in the TST STARR+ group than in the PPH group (4.8 vs. 2.9 cm, 10.2 vs. 4.4 cm3, P &lt; 0.05).ConclusionThe TST STARR+ procedure can remove more hemorrhoidal tissue than PPH and it is better suited for patients with severe annular prolapsed hemorrhoids greater than half of the CAD. It has the advantages of convenient to operate, rapid recovery, fewer complications, and long-term satisfactory results.
Characterization of PISTILLATA-like Genes and Their Promoters from the Distyly Fagopyrum esculentum
Authors: You Wei , Chen Xiangjian , Zeng Lingtian , Ma Zhiyuan , Liu Zhixiong 
Published: April 2022

Arabidopsis PISTILLATA (PI) encodes B-class MADS-box transcription factor (TF), and works together with APETALA3 (AP3) to specify petal and stamen identity. However, a small-scale gene duplication event of PI ortholog was observed in common buckwheat and resulted in FaesPI_1 and FaesPI_2. FaesPI_1 and FaesPI_2 were expressed only in the stamen of dimorphic flower (thrum and pin) of Fagopyrum esculentum. Moreover, intense beta-glucuronidase (GUS) staining was found in the entire stamen (filament and anther) in pFaesPI_1::GUS transgenic Arabidopsis, while GUS was expressed only in the filament of pFaesPI_2::GUS transgenic Arabidopsis. In addition, phenotype complementation analysis suggested that pFaesPI_1::FaesPI_1/pFaesPI_2::FaesPI_2 transgenic pi-1 Arabidopsis showed similar a flower structure with stamen-like organs or filament-like organs in the third whorl. This suggested that FaesPI_2 only specified filament development, but FaesPI_1 specified stamen development. Meanwhile, FaesPI_1 and FaesPI_2 were shown to function redundantly in regulating filament development, and both genes work together to require a proper stamen identity. The data also provide a clue to understanding the roles of PI-like genes involved in floral organ development during the early evolution of core eudicots and also suggested that FaesPI_1 and FaesPI_2 hold the potential application in bioengineering to develop a common buckwheat male sterile line.
Presencia de las revistas de ciencias administrativas en el catálogo Latindex 2.0: Estudio de caso
Authors: Párraga Mendoza José Adalberto , Sánchez Rivera María Teresa , Rivero Torres Carlos , Loor Bravo Lucia 
Published: April 2022

Las universidades generan producción científica, estas se hacen accesibles y visibles una vez que son publicadas e indizadas en bases de datos, por lo que resulta imprescindible medir la producción, la visibilidad y el impacto de estas en los centros de educación superior, el punto de partida de esta investigación es identificar la presencia de revistas de la disciplina ciencias administrativas, publicadas por universidades en el Ecuador, en el periodo (2015-2020) e indexadas en Catálogo Latindex 2.0. La metodología empleada para esta investigación es de carácter cuantitativa retrospectivo y apoyado en el método experimental y demostrativo, para referenciar las teorías científicas, se apoya la investigación en cuatro criterios de selección la indexación en el catálogo Latindex 2.0, filiación institucional de la revista a un centro de educación superior, número de artículos por número, e idioma que publica.
Palabras Clave: Administración de empresas; centro superior y de investigación; crecimiento y productividad.
Relación entre la edad y el estrés durante la pandemia en el Ecuador; propuesta de estrategias turísticas
Authors: Abarca Zaquinaula Manuel Antonio , Mendoza Poma Matius Rodolfo , Alvarez Lema Freddy Anaximandro , Sampedro Arrieta Milton Alberto 
Published: April 2022

El objetivo de este trabajo fue, analizar la correlación existente entre el estrés en tiempos de pandemia y la edad de las personas, a través de la estadística inferencial para proponer estrategias turísticas. Las variables de estudio fueron: nivel de estrés y edad, la muestra estuvo conformada por 4220 personas de 19 provincias del Ecuador. Se aplicó un cuestionario en google forms empleando la técnica de encuesta. El análisis estadístico de los datos se realizó con base en el programa estadístico SPSS versión 23 y la realización de la prueba estadística no paramétrica correspondiente “Correlación de Spearman”, con un nivel de confianza al 95% y un nivel de significancia del 5 %. Se concluyó que los más afectados en términos de estrés son las personas de entre 19 y 28 años de edad y que el turismo debe desarrollarse con un enfoque de anti-estrés de la demanda de manera constante.

Palabras clave: estrés, edad, turismo, estrategias turísticas, COVID-19
PENGARUH ITO, RECEIVABLE TURNOVER, FAT DAN TATO TERHADAP PROFITABILITAS PADA PERUSAHAAN CONSUMER GOODS INDUSTRY YANG TERDAFTAR DI BEI PERIODE 2017-2019
Authors: Sridefi Septiani 
Published: April 2022

This study aims to determine the effect of ITO,Receivable Turnover,Fat and TATO on Protability in Consumer Good Industry Companies listed on the Indonesia stock Exchange in 2017-2019.The research method used is a quantitative approach.The population used is manufacturing Companies listed on the Indonesia stock Exchange in 2017-2019 with 51 companies.The sample in this study were 24 Companies with certain criteria.The statistical method used is logistic regression analysis using SPSS 25.The result is that Fixed Asset Turnover has an influence on profitability.Inventory Turnover,Receivable turnover and total asset Turnover have no effect on profitability.

Keywords : Inventory Turnover, Receivable turnover, FAT, and TATO, profitability
Pengaruh Pelatihan Dan Disiplin Terhadap Kinerja Karyawan Pada PT.Angkasa Pura II (PERSERO) Bandara Kualanamu Sumatera Utara
Authors: Try Try Aprina Br Bangun 
Published: April 2022

The object the study is PT. Angkasa Pura II wichh is a companyengaged in avition services. The objective of the study is to test and analize the effect of training and discipline on employee performance at PT. Angkasa Pura II both partially and simultaneously. The research used some theories of training, disipline parrtially and employee performance whivh were tested by using multiple linear regression analysis. The research used a quantitive research approach, this type of researchis descriptive quantitative an the nature of this study is an exsplonary relationship. The research population were employees at PT. Angkasa Pura II with a total of 150 employees. The research samples were drawn by using the Slovin formula so that the samples obtained for the regression test were 109 employees and 30 respondents were used for testing the validity and reliability. The results of the study showed that simultaneously, training and dicipline had positive and significant effect on employee performance at PT. Angkasa Pura II (Persero). Partially, training and discipline have a positive and significant effect employee performance at PT. Angkasa Pura II (Persero). The coefficient of determination test results showed that 73,6% of the variations in the dependent variable of employee performace were affected by training and discipline and the reamining 26,4% explained by other variables not investigated in the research.
PENGARUH MODAL KERJA, AKTIVA TETAP, DAN PENJUALAN BERSIH TERHADAP LABA BERSIH PADA PT PERKEBUNAN NUSANTARA III (PERSERO) TAHUN 2012-2015
Authors: TERE TERESA DERISTA MAULINA GIRSANG 
Published: April 2022

Laporan keuangan menyajikan data yang bersifat umum yang merupakan suatu daftar atau laporan keuangan. Laporan keuangan tersebut menunjukkan aktivitas perusahaan dalam suatu periode tertentu. Untuk mendapatkan informasi yang handal maka perusahaan memerlukan suatu struktur pengendalian yang tepat. Tujuan diadakannya penelitian ini adalah untuk menguji dan menganalisis bagaimana pengaruh Modal Kerja, Aktiva Tetap, dan Penjualan Bersih terhadap Laba Bersih pada PT.Perkebunan Nusantara III (Persero) Tahun 2012-2015. Populasi yang digunakan dalam penelitian ini adalah laporan keuangan PT.Perkebunan Nusantara III (Persero) yang merupakan penggabungan dari 3 (tiga) BUMN perkebunan yang terdiri dari PT.Perkebunan Nusantara IV, dan PT.Perkebunan Nusantara V yang pengelolaannya disatukan kedalam satu manajemen. Dengan menggunakan teknik Sampling jenuh, Metode yang digunakan dalam penelitian ini adalah kuantitatif, jenis penelitian yang digunakan adalah deskriptif, sifat penelitian ini adalah kausal dan jenis data adalah sekunder. Metode analisis data yang digunakan adalah regresi berganda. Teknik pengumpulan data menggunakan teknik dokumentasi dilakukan untuk mengumpulkan data sekunder dari PT.Perkebunan Nusantara III (Persero) Tahun 2012-2015. Hasil penelitian ini menunjukkan bahwa Modal Kerja, Aktiva Tetap, dan Penjualan secara simultan berpengaruh positif terhadap Laba Bersih pada PT.Perkebunan Nusantara III (Persero) Tahun 2012-2015 . Secara parsial Modal Kerja berpengaruh positif dan signifikan terhadap Laba Bersih. Secara parsial Aktiva Tetap berpengaruh positif dan signifikan terhadap Laba Bersih. Secara parsial Penjualan Bersih berpengaruh positif dan signifikan terhadap Laba Bersih. Hasil uji koefisien determinasi menunjukkan 81,1% dari variabel Laba Bersih yang dapat dijelaskan oleh variable Modal Kerja, Aktiva Tetap, dan Penjualan.