bluuebunny/crossref_metadata_2025_split huggingface dataset:
270 Sexually Transmitted Infections in Sexually Compulsive Women
Authors: Bubola Lima NR , Nisida I , Sant'Ana do Amaral ML , Scanavino MDT 
Published: May 2022

ABSTRACT

Introduction
There is a large evidence in literature associating compulsive sexual behavior (CSB) with negative outcomes, including increased risk of sexually transmitted infections (STI), unwanted pregnancies, work or educational role impairment, excessive financial expenses, and others. Most research supports that CSB is substantially more frequent among men than women, which could explain the paucity of data about distribution, accurate clinical picture and particularities in this population.

Objective
we proposed an investigation of lifetime reported serological tests and STI in women who are outpatients of a compulsive sexual behavior clinic in Sao Paulo, Brazil.

Methods
we conducted a cross-sectional analysis of data from women who sought treatment in the clinic between 2012 to 2019. Participants are submitted by throughout clinical evaluation to assessing sexual compulsivity severity. Data of lifetime STI, previous treatment and serological tests are asked by a semi-structured interview. The participants who met diagnostic criteria for sexual preference disorder, sexual identity disorder, schizophrenia or other psychotic disorders, hypomanic or manic episode of mood disorder and other mental disorders due to brain dysfunction, injury or physical illness were excluded.

Results
thirty-two women completed baseline evaluation. Of this, twenty-two (68%) met criteria to CSB and twelve (32%) did not. There was no difference in mean of age, proportion of sexual orientation, race, marital status, years of education or income (TABLE 1). Most of women were white, heterosexual and weren't in a stable relationship. The median (Md) of Sexual Compulsivity Scale (SCS) score had significant difference (Md 32,5 IQR [26; 36] to sexually compulsive and Md 11,5 IQR [10,5; 14] to non sexually compulsive; p&lt;0.05) (TABLE 2). Self-reported history of STI was present in 40% of compulsive women while 8% of non-compulsive reported it (p 0.75). About 91% of compulsive and 75% of non-compulsive women reported previous serological tests.

Conclusion
Sexually compulsive women reported more STI than non sexually compulsive women. We believe that small sample size was determinant to do not find statistical difference between the groups due the low statistical power of the test. Further research with greater sample is needed to confirm this inference. However, sexually compulsive women presented high severity of sexual compulsivity which support our inference. The study points out to the importance of psychiatrist to assess STI in sexually compulsive women.

Disclosure
Work supported by industry: no.
271 Synergistic Effect of Berberine and Tea Tree Oil Against Neisseria Gonorrhea, in Vitro T84 Cell Mucosa Model
Authors: Cho M , Wang L , Chang F 
Published: May 2022

ABSTRACT

Introduction
Neisseria gonorrhea could spread through oral sex located at the genitals with one who has pharyngeal gonorrhea—and becoming the most common cause of gonorrhea urethritis in the outpatient clinic. Previous studies revealed oral chlorhexidine rinse could reduce the transmission of gonorrhea. However, chlorhexidine gluconate oral rinses may cause: 1) an increase in staining of teeth and other oral surfaces; 2) an increase in calculus formation; and 3) an alteration in taste perception. In literature reviews, tea tree oil-containing mouth rinses are the most common alternative in treating or preventing oral infectious diseases, such as gingivitis.

Objective
The study aimed to demonstrate if tea tree oil alone or combines with other safe, natural product extracts are effective and could be a suitable alternative to chlorhexidine in preventing oral gonorrhea from spreading.

Methods
We use in vitro T84 cells as mucosa model and Neisseria gonorrhea strain MS11 by testing minimal inhibitory concentration and bacterial adherence, invasion, and transmigration across mucosal layer. In addition, we've tested Berberine, an active alkaloid ingredient extract from Coptis Chinensis, in the absence or presence of the tea tree oil in the MS11 MIC test, epithelial cell viability test using ceftriaxone as control.

Results
We found the minimal inhibitory concentration in Tea tree oil, Berberine, and Ceftriaxone in MS11 strain is 0.2%, 5 μg/ml, and 0.0125 μg/ml . However, when combine the Tea tree oil and barberine, the MIC decreased to 0.000625% Tea tree oil + 1.25μg/ml Berberine. The result showed the highest protective effect from tea tree oil with Berberine in the MIC as well as infectivity. No cell death was observed in 0.2% diluted tea tree oil, 5 μg/ml Berberine, and 0.000625% Tea tree oil + 1.25μg/ml Berberine in epithelial cell viability test.

Conclusions
Berberine, an alkaloid extract from Coptis Chinensis, shows a synergistic effect when combining with tea tree oil against Neisseria gonorrhea MS11 strain in the T84 cell mucosa model. It may serve as an alternative choice from chlorhexidine gluconate.

Disclosure
Work supported by industry: no.
280 Male to Female Gender Reassignment Surgery; Developing Personalised Outcomes to Minimise Complications
Authors: Attwell-Heap A , Duncan C 
Published: May 2022

ABSTRACT

Introduction & Objectives
The Diagnostic and Statistical Manual of Mental Disorders has defined gender dysphoria as incongruence between gender identity and birth-assigned gender. Whilst the definition has been evolving in recent times, the first practice of Gender Reassignment surgery hates back to the mid-late 16th century. Over time, more sophisticated techniques have developed to customise techniques to the individual to minimise post operative complications and ensure patient satisfaction.

Methods
A review of the literature was performed on pubmed with key word searches; Gender Dysphoria, Gender Reassignment Surgery and history.

Results
Gender affirming surgery was first described in the mid 16th century, however precise documention of techniques was not seen until 1931 when the first gender affirming vaginoplasty was performed. Split skin grafts from the back, thigh or buttock were used for vaginoplasty, and less frequently, from intestines. It was Paul Fogh-Anderson in 1953 that widely publicised transformation on George Jorgensen in Denmark and popularised the penile skin graft neovagina. This technique of penile skin inversion was first used in Casablanca, Morocco in 1956. The intestinal vaginoplasty technique was described in the 1900s but not utilised in transgender vaginoplasty until 1974. Throughout the 80s, non genital skin flaps from the medial thigh were used. These multitude of techniques have meant that current practice goals are to construct a neopvagina individualised to patients needs. For patients wanting a functional rather than an asthetic, the neovagina should ideally be self-lubricating and with compliance allowing penetration. Currently, the recognised practices for creation of neovagina are penile inversion, skin grafts, local flaps and bowel vaginoplasty. As long term surgical outcome data for GRS is minimal, institutions should be encourage to gather long term data to aid in personalising the surgical approach to the individual patient.

Conclusions
When counselling patients for Gender affirming surgery, it is important to understand the patients’ needs to help minimise potential complications. Different surgical techniques of male to female GRS have been described and evolved over the 20th century to the point of current best practice. Long term data will enable more personalisation and risk minimisation measures to ensure patient satisfaction.

Disclosure
Work supported by industry: no.
003 The Advance Sling and Male Sexual Function: A Prospective Analysis on the Impact of Pelvic Mesh on Erectile and Orgasmic Domains in Sexually Active Men with Post-Prostatectomy Stress Urinary Incontinence
Authors: Chung E. , Wang J. 
Published: May 2022

ABSTRACT

Introduction
The transvaginal pelvic mesh surgery has been shown to cause female sexual dysfunction. Given this pelvic mesh concern in women, there is similar concern whether men who received pelvic mesh for postprostatectomy stress incontinence (PPSI) may suffer from sexual dysfunction too.

Objective
To evaluate the sexual function impact of AdVance pelvic male sling (PMS) on erectile and orgasmic domains.

Methods
A review of a prospectively collected database was conducted in all sexually active men who underwent AdVance MS for PPSI between 2012 and 2018. Patient demographics, clinical outcomes and validated questionnaires such as the Brief Sexual Function Inventory (BSFI), International Index of Erectile Function (IIEF) and Patient Global Impression of Improvement (PGI-I) were reviewed at 6, 12 and 48 months.

Results
Of the total of 65 men who received AdVance PMS, an increase in IIEF-5 score ≥ 5 points were reported in 10 (15%) patients with no patients complained of deteriorating erectile function at 24 months. The improvement in orgasmic function (Question 10 on IIEF-15) was reported in 22 (34%) patients while 3 (5%) patients reported lower orgasmic score. The sexual domains scores in BMSFI were higher in 40 (62%) patients especially with regards to sexual desire (Question 1 and 2) and satisfaction with sex life (Question 11). No patient reports pelvic or urethral pain. The reported improvement in erectile and orgasm scores remained similar throughout the 24-months follow-up period. All 20 patients with preoperative climacturia reported resolution of their symptoms during sexual activity. The overall PGI-I was 1.4 (1-3) and men with a higher continence rate were more likely to report better male sexual function (p=0.044).

Conclusions
Despite the reported adverse outcomes of pelvic mesh in female sexual dysfunction, the AdVance PMS appears to improve male sexual function with regards to erectile and orgasm domains.

Disclosure
Work supported by industry: no.
024 Effects of the Light-Controlled Nitric Oxide Donor “NORD-1” and Light Irradiation in Rats with Neurogenic Erectile Dysfunction
Authors: Hotta Y. , Mori T. , Ieda N. , Kataoka T. , Nakagawa H. , Kimura K. 
Published: May 2022

ABSTRACT

Introduction
Radical prostatectomy often causes erectile dysfunction (ED). This is considered to be due to surgery-induced nerve damage. Impairments in neurotransmission result in the release of nitric oxide (NO) in penile corpus cavernosum insufficiency. Therefore, phosphodiesterase-5 inhibitors are not necessarily effective for this type of ED. NO supplementation may be effective for this type of ED. However, NO acts on the whole body and thus may cause adverse effects. Recently, we have developed a light-controlled NO donor that enables spatially and temporally controlled NO release by light irradiation. Whether this type of NO donor is useful for neurogenic ED remains unknown.

Objective
We investigated whether this light-controlled NO donor “NORD-1” and light irradiation were effective for managing neurogenic ED in rats with bilateral cavernous nerve injury (BCNI).

Methods
We used eight-week-old male Wistar-ST rats. The rats were divided into four groups: Sham+SiR650 (control compound) (n=5), Sham+NORD-1 (n=5), BCNI+SiR650 (n=6), and BCNI+NORD-1 (n=6). Initially, sham or BCNI surgery was performed under anesthesia in each group. BCNI surgery was performed by crushing the bilateral cavernous nerves. Four weeks post-surgery, erectile function before and after the injection of each compound into the penile corpus cavernosum was evaluated based on the change in intracavernous pressure (ICP) under electrical stimulation of the cavernous nerve. The changes in ICP with and without light irradiation were also evaluated after the administration of each compound.

Results
Before administering each treatment without light irradiation, the ICP/mean arterial pressure (MAP) ratio under stimulation in the BCNI+SiR650 and BCNI+NORD-1 groups were lower than those in the Sham+SiR650 (P&lt;0.01) and Sham+NORD-1 (P=0.06) groups. After the each treatment without light irradiation, the ICP/MAP ratio under stimulation in the Sham+NORD-1 group was slightly higher than that in the Sham+SiR650 group. However, the ICP/MAP ratio under stimulation in the BCNI+SiR650 and BCNI+NORD-1 groups remained significantly lower (P&lt;0.05 and P&lt;0.01, respectively). After the treatment with light irradiation, the ICP/MAP ratios under stimulation in the Sham+NORD-1 and BCNI+NORD-1 groups were significantly higher than those in the Sham+SiR650 (P&lt;0.01) and BCNI+NORD-1 (P&lt;0.01) groups, respectively.

Conclusions
The combination of NORD-1 and light irradiation may be useful for treating neurogenic ED, although its toxicity must be evaluated.

Disclosure
Work supported by industry: no.
040 Central Mechanisms Contribute to Pro-Ejaculatory Response Induced by the Combination of Dopamine and 5-HT2 Receptor Agonist in Rats
Authors: Yoshizumi M. , Yonezawa A. , Kimura Y. , Watanabe C. , Kawatani M. , Sakurada S. , Mizoguchi H. 
Published: May 2022

ABSTRACT

Introduction
Although we previously demonstrated in rats that combination of a dopamine (DA) receptor agonist and 5-HT2 receptor agonist potently and selectively facilitates the ejaculatory response through the activation of D2-like and 5-HT2C receptors, these central mechanisms are not fully understood. We therefore investigated to clarify the target level of the pro-ejaculatory effects by combination of these agonists.

Methods
The ejaculatory response was evaluated 30 min after drug administration by the presence of seminal materials on the paper towel as well as that adhering to the shaft of the penis. In addition, we examined isolated rat seminal vesicle contraction.

Results
Intrathecal administration of 5-HT2 receptor agonist m-CPP (10 μg) but not in intracerebroventricular evoked the synergistic action for ejaculation in the combination with systemic administrated DA receptor agonist apomorphine (0.1 mg/kg, s.c.). Synergy effects of combination were completely inhibited by intrathecal 5-HT2C receptor antagonist SB242084 (10 μg). Intrathecal or intracerebroventricular apomorphine (1-10 μg) evoked the pro-ejaculatory effects in the combination with systemic administrated m-CPP (0.3 mg/kg, i.p.) The selective peripherally acting D2-like receptor agonist carmoxirole did not evoked in the combination with m-CPP. Furthermore, the combination of apomorphine and m-CPP did not produce contraction of rat seminal vesicle.

Conclusion
These results suggest that this synergistic action for ejaculation induces in the spinal or supraspinal level but not the peripheral sites. Furthermore, the present study suggests that the activation of the spinal ejaculatory pattern generator through 5-HT2C receptors is synergistically potentiated by both spinal and supraspinal DA receptors.

Disclosure
Work supported by industry: no.
041 Effect of DPP-4 Inhibitors on Erectile Dysfunction Caused by Vincristine in Rats
Authors: Kataoka T. , Sanagawa A. , Hotta Y. , Kimura K. 
Published: May 2022

ABSTRACT

Introduction
Various chemotherapeutic agents are administered to cancer patients worldwide. Previously, an analysis of the US Food and Drug Administration (FDA) Adverse Event Reporting System (AERS) database showed that vincristine (VCR) increased the risk of erectile dysfunction (ED), as reported in the world meeting on Sexual Medicine 2018. As such, countermeasures for late complications after anticancer drug treatment are required. Neuropathy is a well-known side effect of VCR; however, in recent years, it has been reported that DPP-4 inhibitors improve neuropathy.

Objective
To investigate the effect of DPP-4 inhibitors to ED caused by VCR in rats.

Methods
Twelve-week-old male Wistar ST rats were separated into Control, VCR, and VCR+DPP-4i groups. VCR (0.1 mg/kg) was intravenously administered on days 1, 8, 15, and 22 in the VCR and VCR+DPP-4i groups. Two types of long-term preparations, toleragliptin (TGP; 3 mg/kg/week, po.) and omaligliptin (OGP; 3 mg/kg/week, po.), were examined for DPP-4i. Erectile function was tested using intracavernous pharmacotherapy (ICP) measurements, and endothelial function was tested through an isometric tension study. Additionally, mRNA expressions were tested using real-time PCR.

Results
After the 4-week observation period, the ICP/mean arterial pressure (MAP) ratio in the VCR group (0.42 ± 0.04) was significantly decreased compared to the Control group (0.72 ± 0.05) (P &lt;0.01). The ICP/MAP ratio in the VCR+TGP group (0.64 ± 0.02) increased significantly compared to the VCR group (P &lt;0.05), however the ICP/MAP ratio in the VCR+OGP (0.50 ± 0.08) did not increase significantly compared to the VCR group (P &gt; 0.05). Real-time PCR analysis showed that the catalase, VEGF, MCP-1, and PAI-1 mRNA expressions were increased, while the IL-6 mRNA expression decreased significantly in the VCR+TGP and VCR+OGP groups. In contrast, the nNOS mRNA expression level was higher in the VCR+TGP group than in the VCR+OGP group.

Conclusions
In this study, we determined that DPP-4 inhibitors may improve ED by VCR administration. For ED associated with VCR administration, long-term DPP-4 inhibitor TGP administration suggested a significant improvement in erectile function, but OGP did not show a significant improvement effect. An increase in antioxidant activity and a decrease in inflammatory cytokines were observed for both drugs, including a surge in vascular increase factors. In contrast, the nNOS expression level was higher in TGP, suggesting that there may be a difference in the amount of NO production via nerves. Therefore, TGP may be useful for male sexual dysfunction associated with vincristine administration.

Disclosure
Work supported by industry: no.
047 Anti-Inflammatory Effect of Indoleamine 2,3-Dioxygenase 1 Inhibition for Lipopolysaccharide Induced Epididymitis
Authors: Ohira S. , Tone S. , Nakatsuka T. , Morinaka H. , Takasaki H. , Hirata K. , Shimizu S. , Nagai A. 
Published: May 2022

ABSTRACT

Introduction and Objective
Even though antimicrobial therapy is received, some patients with epididymitis remaining inflammatory changes and led to refractory condition. Innovative therapy focused on the inflammatory changes is needed for epididymitis. Indoleamine 2,3-dioxygenase 1 (IDO1) catalyzes the first and rate-limiting step of tryptophan catabolism. IDO1 plays a key role in immune response and shows high expression in male genitalia. Furthermore, protective effect of IDO1 inhibition for various inflammatory disease was indicated in some reports. We hypothesize that IDO1 plays a central role for immunological reaction in epididymal inflammation. We investigated the relevance between IDO1 inhibition and epididymal inflammation. This study was approved by the safety committee for DNA experiments and the animal research committee of our facility (Approval No.14-35, 18-52).

Materials and Methods
Ten to twelve weeks old, C57BL/6 IDO1 knock out (IDO1 KO) mice were used through the research. Same conditional wild type (WT) mice were set as control. Lipopolysaccharide (LPS) 5μg/g(weight) was injected into epididymis from the vas deferens side. Epididymis was removed at a to 7 days after epididymitis modeling. Inflammatory changes in epididymis were investigated using histological, biochemical and immunohistochemical analysis. Series of these analysis were duplicated at least.

Results
Histological analysis using HE staining showed decreased effect of inflammation in IDO1 KO mice compared with WT mice. Proteomic analysis showed decreased effect of both inflammatory promoting cytokines (IL-1α, IL-6) and chemokines (CCL3, CXCL1) in IDO KO mice. On the other hand, increased effect of inflammatory inhibiting cytokines (IL-4, IL-10) were showed in IDO KO mice. Same results were obtained from immunohistochemical analysis using immunofluorescent staining.

Conclusions
IDO1 is involved in epididymal inflammation via cytokine and chemokine. IDO1 inhibition contribute to tissue protection for epididymitis. Therefore, IDO1 inhibition might be an innovative therapy for epididymitis.

Disclosure
Work supported by industry: no.
066 Haemospermia in the Real-Life Setting: Challenging the EAU Guidelines
Authors: Pozzi E. , Ventimiglia E. , Fallara G. , Boeri L. , Belladelli F. , Cignoli D. , Candela L. , Capogrosso P. , Schifano N. , Raffo M. , Colandrea G. , Cornelius J. , Mattei A. , Montorsi F. , Salonia A. 
Published: May 2022

ABSTRACT

Introduction
The Sexual and Reproductive Health guidelines of the European Association of Urology (EAU) suggest stratifying patients complaining of haemospermia into low versus high-risk categories for treatable aetiologies.

Objective
To validate EAU guidelines in a homogeneous cohort of men with haemospermia and to identify a novel and better performing risk stratification compared to EAU guidelines.

Methods
Data from 283 consecutive patients complaining of a single episode/recurrent haemospermia were retrospectively analysed. Patients were stratified into low vs. high-risk according to EAU guidelines, whose diagnostic performance was then validated. We identified a new risk stratification model based on clinical factors associated with i) positive semen culture and ii) prostate cancer (PCa) and bladder cancer (BC). Diagnostic accuracy of the two predictive models (EAU vs. New) was assessed and decision curve analyses (DCA) tested their clinical benefit.

Results
Overall, 259 (91.5%) were high-risk and 24 (8.5%) low risk according to the EAU guidelines. Recurrent haemospermia was reported by 134 (47.4%) patients. 126 (44.5%) had baseline CCI score ≥ 1. At MVA logistic regression analysis, history of recurrent genito – urinary tract infections was identified as a predictor for positive semen culture (OR: 3.39, 95% CI: 1.77 – 6.57, p=0.002). Likewise, baseline CCI ≥ 1 was identified as a predictor for PCa and BC (OR: 1.55, 95% CI: 1.17 – 2.04, p=0.009). Sensitivity, specificity, and AUC of the EAU guidelines were 13.3%, 89.2% and 51% respectively, whereas the new model performed substantially better: 98.9%, 58% and 78% respectively.

Conclusion
The application of the EAU risk stratification does not ensure proper identification of high-risk patients complaining of haemospermia. We propose a novel, better performing and easily implementable risk stratification.

Disclosure
Work supported by industry: no.
Determination of the H2O‐Saturated Solidus of Albite by Electrical Conductivity
Authors: Guo Xuan , Li Wan‐Cai , Zhang Li , Ni Huaiwei 
Published: May 2022

AbstractIn the study of subduction zone processes, there has been much debate on slab dehydration against slab melting, as well as on flux melting of the mantle wedge against metasomatism and a prolonged period of storage and heating before mantle melting. H2O‐saturated solidus of rock is critical to the debate. Unfortunately, there is a significant discrepancy in H2O‐saturated solidus between different experimental studies, mainly due to the difficulty in distinguishing hydrous melt from aqueous fluid in quenched experimental products. In this study, we developed a new approach to locating H2O‐saturated solidus of rock systems by monitoring the electrical conductivity of the system in situ. A mixture of albite and >10 wt% H2O was heated stepwise in the range of 0.35–1.7 GPa in a piston cylinder apparatus. A jump in electrical conductivity (up to 1.8 log units) was observed over a small temperature interval (10–20°C). The temperature corresponding to the electrical jump decreased from 790 ± 10°C at 0.35 GPa to 640 ± 10°C at 1.7 GPa, in good agreement with previously reported H2O‐saturated solidus of albite. Examination of the experimental products quenched immediately after observation of the electrical conductivity jump confirmed the occurrence of albite melting. Our experimental results suggest that the electrical conductivity jump serves as a good indicator for rock melting under H2O‐saturated conditions, and this in situ approach can overcome the ambiguity in phase identification in quench experiments. This new approach has the potential to solve the discrepancy over H2O‐saturated solidus of a variety of rock systems and shed light on subduction zone processes.