bluuebunny/crossref_metadata_2025_split huggingface dataset:
Herbivory by European Earwigs (Forficula auricularia; Dermaptera: Forficulidae) onCitrusSpecies Commonly Cultivated in California
Authors: Kahl Hanna M , Mueller Tobias G , Cass Bodil N , Xi Xinqiang , Cluff Emma , Rosenheim Jay A 
Published: February 2022

AbstractAgricultural plant species differ in susceptibility to herbivores; therefore, identifying natural resistances or tolerances to pests can be leveraged to develop preventative, integrated pest management approaches. While many Citrus species are grown in California, most pest management guidelines are based upon research conducted on navel oranges [Citrus sinensis (L.) Osbeck; Sapindales: Rutaceae]. A recent study has established European earwigs (Forficula auricularia L.; Dermaptera: Forficulidae) as herbivores of young navel orange fruit, causing damage ranging from small bite marks to large chewed holes. It is unknown whether earwigs damage fruit of other citrus species. We conducted field experiments in which we caged earwigs to branch terminals bearing young fruit to explore potential differences in susceptibility of Citrus species to European earwigs. Specifically, we tested whether three species, navel oranges, clementines (C. clementina hort. ex Tanaka), and true mandarins (C. reticulata Blanco) exhibit differences in: 1) feeding deterrence to earwigs; 2) suitability as food for earwigs; 3) preferential abscission of damaged fruit; and 4) healing of damaged fruit. Earwigs caused heavy damage on navel orange and clementine fruit, whereas heavy damage was rare on true mandarin fruit. There was little evidence of preferential abscission of damaged fruit or healing of seriously damaged fruit. Consequently, several heavily damaged navel orange and one clementine fruit were retained to harvest and developed large scars. Overall, we found that Citrus fruit vary in their susceptibility to earwigs, and pest management strategies for earwigs should be refined to consider their varying effects on different Citrus species.
Change Your Face, Change Your Life? Prison Plastic Surgery as a Way to Reduce Recidivism
Authors: Pearl Sharrona 
Published: February 2022

Abstract
The paper explores the history and ethics of prison plastic surgery programs, which ran from the 1950s through as late as 1988 in the UK, the US, and Canada. I focus in particular on the Oakalla Prison, the Haney Young Offenders Correctional Unit, and the Kingston Penitentiary in Canada; the Huntsville Penitentiary in Texas; the Camp Hill Borstal in England; and the collaboration between Montifiore Hospital and Sing-Sing Prison in New York. Sometimes federally funded, these programs were designed to reduce rates of recidivism, operating under the notion that a changed face could lead to a changed character. The surgeries were rooted in a commitment to rehabilitation through medicine, offering participants access to surgery in exchange for good behavior, participation in an experimental protocol, and in some cases, providing training for medical students and residents. As I show, these programs were consonant with prevailing experimental and ethical ethos, and maintain deep continuity with the idea that changes in appearance could lead to changes in behavior.
Ukraine and the International Criminal Court
Authors: Masol Sergii 
Published: February 2022

Abstract
Ukraine exhibits two, seemingly contradictory, attitudes towards the International Criminal Court (ICC). On the one hand, it lodged two ad hoc declarations, accepting the ICC’s jurisdiction over certain alleged crimes occurring on its territory since late 2013; on the other hand, the desirability of ratifying the Rome Statute of the International Criminal Court remains an open question. This problem is exacerbated by the highly contested information environment, as well as by the widespread misunderstanding of the ICC, its functioning, and its likely impact on events in Ukraine. The present article examines the heated debate between national stakeholders from the vantage point of post-truth politics. It also explains how the government can use the status quo to keep prospective international investigations and prosecutions under control.
Ewing sarcoma of the facial soft tissues—a case report
Authors: Knight Beau , Brierly Gary , Dimitroulis George 
Published: February 2022

Abstract
Ewing sarcoma (ES) is an uncommon malignancy of the maxillofacial region, primarily affecting the long bones and pelvis of the paediatric population. Within the head and neck, the facial skeleton is responsible for the majority of reported cases. ES poses a challenge to Oral and Maxillofacial Surgeons due to its rarity and its aggressive nature. This case report is of a 35-year-old female with primary ES in the buccal soft tissues—the first reported case of its kind.
A rare case of internal hernia through the foramen of Winslow
Authors: Monteiro Emerson Leonardo , Andresen Julian Ramin , Schmid Johannes , Mischinger Hans Jörg , Kornprat Peter 
Published: February 2022

Abstract
Internal hernia is a rare cause of intestinal obstruction. The risk of internal herniation is higher in some patients after abdominal surgery or with certain anatomical anomalies. Here, we present a case of an 80-year-old patient with incarcerated internal hernia through the foramen of Winslow due to an anatomical abnormality.
Identification of OsPK5 involved in rice glycolytic metabolism and GA/ABA balance for improving seed germination via genome-wide association study
Authors: Yang Bin , Chen Mingming , Zhan Chengfang , Liu Kexin , Cheng Yanhao , Xie Ting , Zhu Peiwen , He Ying , Zeng Peng , Tang Haijuan , Tsugama Daisuke , Chen Sunlu , Zhang Hongsheng , Cheng Jinping 
Published: February 2022

Abstract
Seed germination plays a pivotal role in the plant life cycle, and its precise regulatory mechanisms are not clear. In this study, 19 quantitative trait loci (QTLs) associated with rice seed germination were identified through genome-wide association studies (GWAS) of the following traits in 2016 and 2017: germination rate (GR) at 3, 5, and 7 days after imbibition (DAI) and germination index (GI). Two major stable QTLs, qSG4 and qSG11.1, were found to be associated with GR and GI over 2 continuous years. Furthermore, OsPK5, encoding a pyruvate kinase, was shown to be a crucial regulator of seed germination in rice, and might be a causal gene of the key QTL qSG11.1, on chromosome 11. Natural variation in OsPK5 function altered the activity of pyruvate kinase. The disruption of OsPK5 function resulted in slow germination and seedling growth during seed germination, blocked glycolytic metabolism, caused glucose accumulation, decreased energy levels, and affected the GA/ABA balance. Taken together, our results provide novel insights into the roles of OsPK5 in seed germination, and facilitate its application in rice breeding to improve seed vigour.
Violence, Ritual, and Vogue: Black Queer Feminist Praxis in Motion
Authors: Forsgren La Donna L 
Published: February 2022

Abstract
As an unapologetically Black feminist artist, Ntozake Shange furthered the cause of black girl representation on Broadway stages with the 1976 debut of for colored girls who have considered suicide / when the rainbow is enuf. Shange’s award-winning choreopoem eschews the masculinist liberation discourse of the Black Power era and instead centers the concerns of Black girls within the freedom struggle. Using twenty poems interlaced with dance and music, Shange illuminates the subjectivity of seven “colored girls” who experience sexual, emotional, and physical violence in their communities. Committed to the health and safety of the entire Black community, Shange concludes the performance with a ritual dance to foster unity and community healing from violence. Her early Black feminist intervention serves as a foundation for the artistic work of Black Lives Matter activists today, many of whom continue to use ritual performances to promote community healing in the wake of white-authored violence. Black Lives Matter movement artists and activists Gorgeous Mother Karma Gucci, Adonte Prodigy, and Amya Miyake-Mugler, for example, performed ritual Voguing at a Chicago demonstration on 3 June 2020 to bring greater visibility to the intracultural violence reaped upon Black queer and transgender girls. Their ritual Voguing, which I situate as Black queer feminist praxis in motion, reimagines the Black radical tradition as collective liberation. Collectively, the work of Shange, Gucci, Prodigy, and Mugler affirms the vital truth that none of us are free until all of us are free.
CRISPR-iPAS: a novel dCAS13-based method for alternative polyadenylation interference
Authors: Tian Shuye , Zhang Bin , He Yuhao , Sun Zhiyuan , Li Jun , Li Yisheng , Yi Hongyang , Zhao Yan , Zou Xudong , Li Yunfei , Cui Huanhuan , Fang Liang , Gao Xin , Hu Yuhui , Chen Wei 
Published: February 2022

Abstract
Alternative polyadenylation (APA) plays an important role in gene regulation. With the recent application of novel sequencing technology in APA profiling, an ever-increasing number of APA genes/sites have been identified. However, the phenotypic relevance of most of these APA isoforms remains elusive, which is largely due to the lack of a convenient genetics tool for APA interference. To address this issue, herein, an efficient method is developed based on the CRISPR-dCas13 system, termed as CRISPR-iPAS. Out of eight different dCas13 proteins, Porphyromonas gulae (Pgu) dCas13b, is identified as the most effective one in blocking the usage of the polyadenylation site (PAS). With guide RNAs targeting at core regulatory elements, dPguCas13b enabled APA regulation of endogenous genes with different APA types, including tandem 3′UTR, alternative terminal exon, as well as intronic PAS. Finally, we demonstrated that the proposed APA perturbation tool could be used to investigate the functional relevance of APA isoforms.
STRIDE: accurately decomposing and integrating spatial transcriptomics using single-cell RNA sequencing
Authors: Sun Dongqing , Liu Zhaoyang , Li Taiwen , Wu Qiu , Wang Chenfei 
Published: February 2022

Abstract
The recent advances in spatial transcriptomics have brought unprecedented opportunities to understand the cellular heterogeneity in the spatial context. However, the current limitations of spatial technologies hamper the exploration of cellular localizations and interactions at single-cell level. Here, we present spatial transcriptomics deconvolution by topic modeling (STRIDE), a computational method to decompose cell types from spatial mixtures by leveraging topic profiles trained from single-cell transcriptomics. STRIDE accurately estimated the cell-type proportions and showed balanced specificity and sensitivity compared to existing methods. We demonstrated STRIDE’s utility by applying it to different spatial platforms and biological systems. Deconvolution by STRIDE not only mapped rare cell types to spatial locations but also improved the identification of spatially localized genes and domains. Moreover, topics discovered by STRIDE were associated with cell-type-specific functions and could be further used to integrate successive sections and reconstruct the three-dimensional architecture of tissues. Taken together, STRIDE is a versatile and extensible tool for integrated analysis of spatial and single-cell transcriptomics and is publicly available at https://github.com/wanglabtongji/STRIDE.
Global chromosome rearrangement induced by CRISPR-Cas9 reshapes the genome and transcriptome of human cells
Authors: Liu Ying , Ma Guangwei , Gao Zenghong , Li Jian , Wang Jin , Zhu Xiangping , Ma Ruowu , Yang Jiawen , Zhou Yiting , Hu Kaishun , Zhang Yin , Guo Yabin 
Published: February 2022

AbstractChromosome rearrangement plays important roles in development, carcinogenesis and evolution. However, its mechanism and subsequent effects are not fully understood. Large-scale chromosome rearrangement has been performed in the simple eukaryote, wine yeast, but the relative research in mammalian cells remains at the level of individual chromosome rearrangement due to technical limitations. In this study, we used CRISPR-Cas9 to target the highly repetitive human endogenous retrotransposons, LINE-1 and Alu, resulting in a large number of DNA double-strand breaks in the chromosomes. While this operation killed the majority of the cells, we eventually obtained live cell groups. Karyotype analysis and genome re-sequencing proved that we have achieved global chromosome rearrangement (GCR) in human cells. The copy number variations of the GCR genomes showed typical patterns observed in tumor genomes. The ATAC-seq and RNA-seq further revealed that the epigenetic and transcriptomic landscapes were deeply reshaped by GCR. Gene expressions related to p53 pathway, DNA repair, cell cycle and apoptosis were greatly altered to facilitate the cell survival. Our study provided a new application of CRISPR-Cas9 and a practical approach for GCR in complex mammalian genomes.
Programmable manipulation of oligonucleotide–albumin interaction for elongated circulation time
Authors: Yang Cai , Zhao Haitao , Sun Yang , Wang Cheng , Geng Xinyao , Wang Ruowen , Tang Lumin , Han Da , Liu Jianjun , Tan Weihong 
Published: February 2022

Abstract
Oligonucleotide (ON) therapeutics are emerging as a new generation of medicine with tremendous potential, but their clinical translation is hampered by inferior stability and short circulation time in the human body. Here, we report a general approach to manipulating the interaction between ONs and albumin by modulating hydrophobicity. A series of DNA aptamer derivatives were designed and prepared by programmable synthesis as an ON library with a gradient of hydrophobic base ‘F’. In vitro experiments revealed that the introduction of two F bases at both ends of ONs enhanced the biostability without sacrificing biological activities, while the binding affinity toward albumin was dramatically increased with Kd in the range of 100 nM to 1 μM. In vivo imaging confirmed the immediate formation of the aptamer–albumin complex after the injection, and the circulation time of the aptamer was dramatically elongated owing to the enhanced biostability and retarded renal excretion. The programmable incorporation of the F base provides a general approach to regulating albumin-binding affinity and enhancing the stability of aptamers in vivo, conferring aptamer therapeutics prolonged circulation time to meet clinical requirements.
Kinetic regulation of MXene with water-in-LiCl electrolyte for high-voltage micro-supercapacitors
Authors: Zhu Yuanyuan , Zheng Shuanghao , Lu Pengfei , Ma Jiaxin , Das Pratteek , Su Feng , Cheng Hui-Ming , Wu Zhong-Shuai 
Published: February 2022

Abstract
MXenes are one of the key materials for micro-supercapacitors (MSCs), integrating miniaturized energy-storage components with microelectronics. However, the energy densities of MSCs are greatly hampered by MXenes’ narrow working potential window (typically ≤0.6 V) in aqueous electrolytes. Here, we report the fabrication of high-voltage MXene-MSCs through the efficient regulation of reaction kinetics in 2D Ti3C2Tx MXene microelectrodes using a water-in-LiCl (WIL, 20 m LiCl) salt gel electrolyte. Importantly, the intrinsic energy-storage mechanism of MXene microelectrodes in WIL, which is totally different from traditional electrolytes (1 m LiCl), was revealed through insitu and exsitu characterizations. We validated that the suppression of MXene oxidation at high anodic potential occurred due to the high content of WIL regulating anion intercalation in MXene electrodes, which effectively broadened the voltage window of MXene-MSCs. Remarkably, the symmetric planar MXene-MSCs presented a record operating voltage of 1.6 V, resulting in an exceptionally high volumetric energy density of 31.7 mWh cm−3. With the ultra-high ionic conductivity (69.5 mS cm−1) and ultralow freezing point (−57°C) of the WIL gel electrolyte, our MSCs could be operated in a wide temperature range of −40 to 60°C, and worked for a long duration even at −40°C, demonstrative of its practicality in extreme environments.
Comparing polysomnography, actigraphy, and sleep diary in the home environment: The Study of Women’s Health Across the Nation (SWAN) Sleep Study
Authors: Lehrer H Matthew , Yao Zhigang , Krafty Robert T , Evans Marissa A , Buysse Daniel J , Kravitz Howard M , Matthews Karen A , Gold Ellen B , Harlow Sioban D , Samuelsson Laura B , Hall Martica H 
Published: February 2022

AbstractStudy ObjectivesPolysomnography (PSG) is considered the “gold standard” for assessing sleep, but cost and burden limit its use. Although wrist actigraphy and self-report diaries are feasible alternatives to PSG, few studies have compared all three modalities concurrently across multiple nights in the home to assess their relative validity across multiple sleep outcomes. This study compared sleep duration and continuity measured by PSG, actigraphy, and sleep diaries and examined moderation by race/ethnicity.MethodsParticipants from the Study of Women’s Health Across the Nation (SWAN) Sleep Study included 323 White (n = 147), African American (n = 120), and Chinese (n = 56) middle-aged community-dwelling women (mean age: 51 years, range: 48–57). PSG, wrist actigraphy (AW-64; Philips Respironics, McMurray, PA), and sleep diaries were collected concurrently in participants’ homes over three consecutive nights. Multivariable repeated-measures linear models compared time in bed (TIB), total sleep time (TST), sleep efficiency (SE), sleep latency (SL), and wake after sleep onset (WASO) across modalities.ResultsActigraphy and PSG produced similar estimates of sleep duration and efficiency. Diaries yielded higher estimates of TIB, TST, and SE versus PSG and actigraphy, and lower estimates of SL and WASO versus PSG. Diary SL was shorter than PSG SL only among White women, and diary WASO was lower than PSG and actigraphy WASO among African American versus White women.ConclusionsGiven concordance with PSG, actigraphy may be preferred as an alternative to PSG for measuring sleep in the home. Future research should consider racial/ethnic differences in diary-reported sleep continuity.
Exercise mediates ubiquitin signalling in human skeletal muscle
Authors: Lord Samuel O. , Lai Yu‐Chiang 
Published: February 2022

AbstractPhysical activity or regular exercise provides many beneficial effects towards human health, helping prevent and ameliorate metabolic diseases. However, certain molecular mechanisms that mediate these health benefits remain poorly understood. Parker et al. provided the first global analysis of exercise‐regulated ubiquitin signalling in human skeletal muscle, revealing post‐translational modification cross‐talk. As a result of their analysis, NEDDylation is thought to promote ubiquitin signalling for the removal of damaged proteins following exercise. The proteomic dataset generated from their study is invaluable for researchers in this field to validate new mechanistic hypotheses. To further reveal molecular mechanisms regulated by exercise, future research could employ more sensitive mass spectrometry‐based workflows that increase the detection of both ubiquitylated sites and peptides and subsequently identify more exercise‐regulated ubiquitin signalling pathways.
Fundamental Teaching Activities in Family Medicine Framework: Analysis of Awareness and Utilization
Authors: Lee-Krueger Rachelle C. W. , Moreau Katherine , Delva Dianne , Eady Kaylee , Giroux Catherine M. , Archibald Douglas 
Published: February 2022

Introduction:
In 2015, the College of Family Physicians of Canada, in performing their commitment to supporting its members in their educational roles, created the Family Medicine Framework (FTA). It was designed to assist family medicine educators with an understanding of the core activities of educators: precepting, coaching, and teaching within or beyond clinical settings. Given that an examination of member awareness of FTA has not been previously undertaken, our primary objective was to conduct an evaluation on its utility and application.

Methods:
In partnership with College of Family Physicians of Canada Faculty Development Education Committee members, we used a practical participatory evaluation approach to conduct a two-phase mixed-methods evaluation of the FTA. We distributed an electronic survey in French and English languages to Canadian faculty development, program, and site directors in family medicine. We then conducted follow-up interviews with self-selected participants.

Results:
Of the target populations, 12/15 (80%) faculty development directors (FDDs), 12/18 (66.7%) program directors, and 34/174 (19.5%) site directors completed the electronic survey. Subsequently, 6 FDDs, 3 program directors, and 3 site directors completed an interview (n = 12). Findings indicate that awareness of the FTA was highest among FDDs. Facilitators who encourage teachers to use the FTA and barriers for low uptake were also identified.

Discussion:
This evaluation illuminated that varied levels of awareness of the FTA may contribute to the low uptake among education leaders. We also suggest future research to address possible barriers that hinder effective applications of the FTA in faculty development initiatives.
Only When They Seek: Exploring Supervisor and Resident Perspectives and Positions on Upward Feedback
Authors: Ramani Subha , Lee-Krueger Rachelle C. W. , Roze des Ordons Amanda , Trier Jessica , Armson Heather , Könings Karen D. , Lockyer Jocelyn M. 
Published: February 2022

Introduction:
Verbal feedback from trainees to supervisors is rare in medical education, although valuable for improvement in teaching skills. Research has mostly examined narrative comments on resident evaluations of their supervisors. This study aimed to explore supervisors' and residents' beliefs and experiences with upward feedback, along with recommendations to initiate and facilitate effective conversations.

Methods:
Using 60-minute focus group discussions, a previous study explored opinions of internal medicine residents and clinical supervisors at the Brigham and Women's Hospital regarding the impact of institutional culture on feedback conversations. For this study, we conducted a thematic analysis of the transcribed, anonymous data to identify key concepts pertaining only to verbal upward feedback, through the theoretical lens of Positioning theory.

Results:
Twenty-two supervisors and 29 residents participated in three and five focus groups, respectively. Identified themes were mapped to three research questions regarding (1) existing beliefs (lack of impact, risks to giving supervisors feedback, need for preparation and reflection), (2) experiences (nonspecific language, avoidance of upward feedback, bypassing the supervisor), and (3) recommended approaches (setting clear expectations, seeking specific feedback, emphasizing interest in growth).

Discussion:
Study participants appeared to assume learner–teacher positions during feedback conversations, resulting in residents' concerns of adverse consequences, beliefs that supervisors will neither accept feedback nor change their behaviors, and avoidance of constructive upward feedback. Residents suggested that emphasis on mutual professional growth and regular feedback seeking by supervisors could encourage them to take on the role of feedback providers. Their recommendations could be a valuable starting point for faculty development initiatives on upward feedback.