bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychological symptoms in children who are victims of war and migration: Comparison of Turkish and Syrian students
Authors: Çekiç Ali 
Published: September 2022

The aim of this study is to examine whether exposure to war and migration trauma has a long-term effect on psychological symptoms in children. The Brief Symptom Inventory was administered to a total of 207 students, 111 of whom were Syrians and 96 of whom were Turks, studying at a secondary school in the Mamak district of Ankara. The data obtained show that Syrian students have higher average scores in the anxiety, negative self and somatisation sub-dimensions than their Turkish peers. This situation can be considered an important indicator as war and forced migration caused by war negatively affect children’s mental health. As a matter of fact, the effects of post-traumatic mental health disorders in children who have experienced war trauma can be seen even 5 years after the traumatic event. Syrian students whose mental health was evaluated within the scope of this research have been living in Turkey for 4–6 years.

Keywords: Children, victims, psychological symptoms, war, migration;
Pengaruh Ekstrak Etanolik Daun Kelor (Moringa oleifera, Lam) Terhadap Nilai Ekspresi TNF-α Testis Tikus Wistar (Rattus norvegicus) Model Sindrom Metabolik
Authors: Tyas Nur Winarno Putri  , Ratna Budiani Dyah , - Muthmainah 
Published: September 2022

Latar Belakang: Peningkatan angka penderita sindrom metabolik secara global dianggap mengkhawatirkan karena mengakibatkan komplikasi masalah kesehatan pada berbagai organ termasuk organ reproduksi pria. Infertilitas pada pria yang disebabkan oleh inflamasi kronik dan kematian sel yang terjadi pada sindrom metabolik erat kaitannya dengan peran TNF-α sebagai mediator inflamasi. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanolik daun kelor terhadap ekspresi TNF-α pada jaringan testis tikus Wistar model sindrom metabolik.
Metode: Penelitian bersifat ekperimental laboratorik dengan rancangan penelitian posttest only control group design. Subjek tikus Wistar dibagi menjadi 5 kelompok dengan masing-masing kelompok berisi 6 tikus, KI sebagai kontrol normal, KII sebagai kelompok induksi sindrom metabolik, dan KIII, KIV, KV sebagai kelompok sindrom metabolik yang diberi ekstrak etanolik daun kelor dengan dosis secara berurutan 150 mg/kgBB, 250 mg/kgBB, dan 350 mg/kgBB. Induksi sindrom metabolik menggunakan pakan tinggi lemak dan STZ-NA. Perhitungan ekspresi TNF-α menggunakan metode semikuantitatif IDS. Analisis ekspresi TNF-α menggunakan uji One-Way ANOVA yang dilanjutkan post hoc LSD dan uji regresi linear.
Hasil: Uji One-Way ANOVA ekspresi TNF-α pada jaringan testis menunjukkan p=0,001 (p<0,05), maka terdapat perbedaan ekspresi TNF-α yang signifikan pada kelima kelompok. Perbedaan bermakna terdapat pada KI terhadap KII dan KII terhadap KIV dan KV. Uji regresi linear menunjukkan pengaruh dosis ekstrak etanolik daun kelor memberikan arah negatif terhadap ekspresi TNF-α.
Simpulan: Pemberian ekstrak etanolik daun kelor (Moringa oleifera, Lam) dengan dosis 150 mg/kgBB, 250 mg/kgBB, dan 350 mg/kgBB dapat menurunkan ekspresi TNF-α.
Kata Kunci: TNF-α, Ekstrak Etanolik Daun Kelor, Sindrom Metabolik, Testis, Spermatogenesis
Deep Learning Estimation of the Industrial Wood Production Level with Respect to the Natural Harm Factors
Authors: Sevinç Volkan 
Published: September 2022

Abstract

Protection and sustainability of forest assets are possible with planned production of the forest products. Among these products, industrial wood is the most important raw material for various sectors. Thus, the estimation of the industrial wood production level is important to use this product optimally, without disturbing the balance of forest assets. This article aims to estimate the industrial wood production level based on four natural harm factors, which are forest fires, outbreaks of insects, outbreaks of diseases, and severe weather events. The estimation model was built by using a deep learning method, which is an artificial neural network model based on the multilayer perceptron architecture. The study shows that the most harmful factor decreasing the industrial wood production level is outbreaks of diseases. The second effective factor, however, appears to be severe weather events. The third and the fourth factors were determined to be outbreaks of insects and burned forest areas, respectively.
Antioxidant studies of hispolon and its derivatives.
Authors: Saka Vara Prasad , Vellapandian Chitra , Narayanaswamy Damodharan 
Published: September 2022

Abstract
Hispolon, a polyphenolic yellow pigment isolated from Chinese mushrooms which was synthesized by researchers and its derivatives such as monomethyl ether, pyrazole and monomethyl ether pyrazole were evaluated for their chemical stability in cell culture medium, antioxidant effect in Chinese hamster ovarian (CHO) radioprotection in spleen lymphocytes. The results indicate that pyrazole derivatives were more chemically stable in cell culture medium. Further, pretreatment with hispolon and its derivatives in CHO cells showed significant inhibition in the radiation induced Reactive oxygen species (ROS) by pyrazole derivative of hispolon. Similarly, the treatment in the splenic lymphocytes showed no significant expression of antioxidant genes such as γGCL and HO-1, and also no significant radioprotection in the markers like fragmentation of DNA, and pre G1 gated cells. This may be because of the primary nature of lymphocytes with limited life span. In MMP assay hispolon pyrazole and monomethyl ether showed a significant rise indicates that these compounds are protecting through inhibition of radiation induced mitochondrial dysfunction. In conclusion, in line with the previous studies, the pyrazole derivatives of hispolon and hispolon monomethyl ether shows antioxidant and radioprotection.
Evaluation of Arsenic Pollution and Anaerobic Arsenic-metabolizing Bacteria of Lake Van, the World's Largest Soda Lake
Authors: Omeroglu Esra Ersoy , Sudagidan Mert , Ogun Erdal 
Published: September 2022

Abstract
Arsenic is responsible for water pollution in many places in the World and presents a serious health risk for people. Lake Van is in the position of the world's largest soda lake and there are no studies on seasonal arsenic pollution and arsenic resistant bacteria. So, it was aimed to determine arsenic amount of lake water and sediment, to isolate arsenic metabolizing anaerobic bacteria and their identification and determination of arsenic metabolism. Sampling was done from 7.5 meters to represent the four seasons. Metal contents were determined by using ICP-MS. Pure cultures were obtained using the hungate technique. Growth characteristics of the strains were determined at different conditions as well as at arsenate and arsenite concentrations. Molecular studies were also carried out for various resistance genes. Our results showed that Lake Van’s total arsenic amount changes seasonally. As a result of 16S rRNA sequencing, it was determined that the isolates were the members of 8 different genera had arsC resistance gene. In conclusion; for the sustainability of water resources, it is necessary to prevent chemical and microorganism-based pollution. It is thought that the arsenic resistant bacteria obtained as a result of this study will contribute to the solution of environmental arsenic pollution problems, as they are the first data and provide the necessary basic data for the bioremediation studies of arsenic from contaminated environmental habitat. At the same time, the first data that will contribute to the creation of the seasonal arsenic map of Lake Van are obtained.
R- and S-ketamine abrogate lipopolysaccharide-induced depressive-like behavior through microglial modulation: a differential anti-inflammatory and neurotrophic effect of the enantiomers
Authors: Filho Adriano José Maia Chaves , Lós Deniele Bezerra , Soares Michelle , Jucá Paloma Marinho , Queiroz Tatiana , Rodrigues Francisco Eliclécio , Saraiva Karina , Silva Amanda Karolina , Azul Francisco Serra , Leal Luzia Kalyne , Peixoto Christina , Macedo Danielle 
Published: September 2022

Abstract
Depression is a mental disorder associated with increased proinflammatory cytokines and glial activation. Ketamine (KET), a racemic mixture of the R- and S-KET enantiomers, emerged as a rapid antidepressant drug. This study aimed to investigate the microglial modulatory and synaptogenic effects of R- and S-KET in a lipopolysaccharide (LPS)-induced depression model. Male rats were challenged with LPS and, 24h later, received R-KET or S-KET (10 mg/kg, IP). Two h later, these animals were evaluated for depression-like behaviors and adverse psychomimetic effects. Also, BV2 microglial cultures were challenged with LPS and exposed to R- or S-KET. Both enantiomers reversed LPS-induced depression-like behaviors and increased plasma corticosterone. LPS caused intense hippocampal glial activation, demonstrated by microglial (Iba1) and astrocytic (GFAP) markers, accompanied by increased nitrite, pro-inflammatory cytokines TNFα and IL-6, and M1 microglial phenotype markers (iNOS and CD86 mRNA). Both enantiomers attenuated Iba1 and GFAP hippocampal increase, but only S-KET induced an M2 phenotype, indicated by the higher arginase activity, IL-4, IL-10, and expression of M2a response genes (LGLAS3 and IGF-1). In the BV2 cells, S-KET increased arginase activity, IL-4, and IL-10 levels. Both enantiomers reversed the LPS-induced changes in synaptic ultrastructure and synaptosome viability, while only S-KET increased the density and the presence of mature spines in the dentate gyrus. Also, S-KET increased hippocampal BDNF maturation and boosted its secretion by BV2 microglia. Therefore, the present study demonstrated, for the first time, the unique immunomodulatory profile of S-KET, promoting a reparative M2 phenotype and protecting synaptic structures from the LPS-associated damage.
Transcriptomics unravels molecular changes associated with cilia and COVID-19 in chronic rhinosinusitis with nasal polyps.
Authors: Naluai Åsa Torinsson , Östensson Malin , Fowler Philippa , Abrahamsson Sanna , Andersson Björn , Lassesson Stina , Jacobsson Frida , Oscarsson Martin , Bohman Anton , Harandi Ali , Bende Mats 
Published: September 2022

Abstract
Chronic rhinosinusitis with nasal polyps is a common condition where the pathogenesis is largely unknown. We measured total gene expression in nasal mucosa using RNA sequencing technology. Pathways involving “Ciliated epithelial cells” were the most differentially expressed molecular pathways when polyp mucosa and non-polyp mucosa from the same patient was compared (p = 8.5x10− 78). Natural killer T-cell (NKT) (p = 2.4x10− 45) and viral pathways were the most significant when mucosa from patients were compared with mucosa from healthy control individuals. Differentially expressed genes included LZTFL1, XCR1, IFNAR1, IFNAR2 and IL10RB, all located within the strongest genome-wide associated regions of COVID-19. Cilia of nasal epithelial cells have many functions and are likely an important entry point for viral infection. Altered expression of genes related to cilia, NKT-cells and viruses, point to the deregulation of viral defenses in nasal polyps of chronic rhinosinusitis, and may give clues to future intervention strategies.
Pig waste derived capped and non-agglomerated silver nanoparticles and its effect on pathogenic bacteria and physiological responses of fish under multiple abiotic stresses
Authors: Lamare Sowa o , Krishnani K. K. , Kumar Neeraj , Pathak Madhuri , Upadhyay Ajay , Sarkar Biplab , Chadha NK 
Published: September 2022

Abstract
The present study elucidates the extracellular synthesis of capped silver nanoparticles (Ag-NPs) using pig processing waste, mainly the intestine in distilled water incubated with silver nitrate by dispensing with the addition of any capping agent and the advantage of avoiding the agglomeration and loss of nanosized characteristics of AgNPs. The synthesis of Ag-NPs was ascertained by a UV-VIS spectrophotometry of the incubated suspension of yellowish-brown at 410 nm. The Ag-NPs were further characterized using a High-Resolution Transmission Electron Microscope (HRTEM), which confirmed that the Ag-NPs were primarily spherical and had a size range of 5–100 with a maximum frequency fall within 5–30 nm. In addition, the Ag-NPs are characterized by Dynamic Light Scattering (DLS), which confirmed the high stability of the Ag-NPs with a zeta potential of -32 mV. Biomolecules and biological extracts of pig waste act as biogenic reducing and capping agents. Based on the zone of inhibition achieved through the agar well diffusion method, the biosynthesis Ag-NPs showed high bactericidal properties against Aeromonas hydrophila, Edwardsiella tarda, and Micrococcus luteus. The chronic toxicity analysis of the biosynthesized Ag-NPs on Pangasianodon hypophthalmus was carried out using stress biomarkers such as an antioxidant enzyme, AChE, and metabolic enzyme activity. Chronic toxicity of synthesized Ag-NPs was found to increase with increased sub-lethal ammonia concentration and temperature. The findings of this study revealed that biosynthesis of capped and non-agglomerated Ag-NPs can be undertaken by using pig wastes for their potential application in aquaculture based on the properties observed in characterization, bactericidal activity, and physiological responses of the fish.
A strategy for evaluating antiviral resistance: Application to small molecule drugs/inhibitors against SARS-CoV-2
Authors: Sargsyan Karen , Mazmanian Karine , Lim Carmay 
Published: September 2022

Abstract
Alterations in viral fitness cannot be inferred from only mutagenesis studies of an isolated viral protein. To-date, no systematic analysis has been performed to identify mutations that improve virus fitness and reduce drug efficacy. We present a generic strategy to evaluate which viral mutations will diminish drug efficacy and applied it to assess how SARS-CoV-2 evolution may affect the efficacy of current approved/candidate small-molecule antivirals for Mpro, PLpro, and RdRp. For each drug target, we determined the drug-interacting virus residues from available structures and the selection pressure of the virus residues from the SARS-CoV-2 genomes. This enabled the identification of promising drug target regions and small-molecule antivirals that the virus can develop resistance. Our strategy of utilizing sequence and structural information from genomic sequence and protein structure databanks can rapidly assess the fitness of any emerging virus variants and can aid antiviral drug design for future pathogens.
Design, structure and plasma binding of ancestral β-CoV scaffold antigens
Authors: Syren Per-Olof , Hueting David , Schriever Karen , Zuo Fanglei , Du Likun , Persson Helena , Hofström Camilla , Ohlin Mats , Wallden Karin , Hammarstrom Lennart , Marcotte Harold , Pan-Hammarstrom Qiang , Andrell Juni 
Published: September 2022

Abstract
The pandemic caused by Severe acute respiratory syndrome coronavirus 2 has had devastating consequences on global health and economy. Despite the success of vaccination campaigns emerging variants are of concern and novel viruses with the potential to drive future pandemics are circulating in nature. Development of vaccines can be challenging, as key viral protein antigens can be unstable or aggregate. In this study, we present the application of ancestral sequence reconstruction on coronavirus spike protein, resulting in stable and highly soluble ancestral scaffold antigens (AnSAs). The AnSAs interacted with plasma of patients recovered from COVID-19 but did not bind to the human angiotensin-converting enzyme 2 (ACE2) receptor. Cryo-EM analysis of the AnSAs yielded high resolution structures (2.6-2.8 Å) indicating a closed pre-fusion conformation in which all three receptor-binding domains (RBDs) are facing downwards. This captured closed state is stabilised by an intricate hydrogen bonding network mediated by well-resolved loops, both within and across monomers, tethering the N-terminal domain and RBD together, which determines their relative spatial orientation. Finally, we show how AnSAs are potent scaffolds by replacing the ancestral RBD with the Wuhan wild-type sequence, which restored ACE2 binding and increased the interaction with convalescent plasma. In contrast to rational antigen design depending on prior structural knowledge, our work highlights how stable and potentially interesting antigens can be generated using exclusively available sequence information.
Surprising effects of cascading higher order interactions
Authors: Hsieh Hsun-Yi , Vandermeer John , Perfecto Ivette 
Published: September 2022

Abstract
Most species are embedded in multi-interaction networks. Consequently, theories focusing on simple pair-wise interactions cannot predict ecological and/or evolutionary outcomes. This study explores how cascading higher-order interactions (HOIs) would affect the population dynamics of a focal species. Employing a system that involves a myrmecophylic beetle, a parasitic wasp that attacks the beetle, an ant, and a parasitic fly that attacks the ant, the study explores how none, one, and two HOIs affect the parasitism and the sex ratio of the beetle. We conducted mesocosm experiments to examine these HOIs on beetle survival and sex ratio and found that the 1st degree HOI does not change the beetle’s survival rate or sex ratio. However, the 2nd degree HOI significantly reduces the beetle’s survival rate and changes its sex ratio from even to strongly female-biased. We applied Bayes’ theorem to analyze the per capita survival probability of female vs. male beetles and suggested that the unexpected results might arise from complex eco-evolutionary dynamics involved with the 1st and 2nd degree HOIs. Field data suggested the HOIs significantly regulate the sex ratio of the beetle. As the same structure of HOIs appears in other systems, we believe the complexity associated with the 2nd degree HOI would be more common than known and deserve more scientific attention.
N,P-co-doped carbocatalyst from olive pomace obtained by catalytic hydrothermal carbonization for efficient dye degradation via persulfate-based advanced oxidation process
Authors: OUADRHIRI Faiçal EL , Adachi Abderrazzak , Elyemni Majda , Bayout Abdelilah , Hmamou Anouar , Bendaoud Ahmed , Lhassani Abdelhadi , Chaouch Mehdi , Oturan Mehmet Ali , Lahkimi Amal 
Published: September 2022

Abstract
The strategy of doping carbocatalysts with heteroatoms has proven its effectiveness in degrading organic pollutants by persulfate-based advanced oxidation processes. However, research on the effect of doping phosphorus atoms on the degradation performance remains very limited. In this work, a new N, P-doped carbocatalyst (N,P-HC) is designed by hydrothermal carbonization (HTC) followed by pyrolysis at 700°C using a biowaste (olive pomace) as a carbon source to degrade organic pollutants in the presence of peroxydisulfate (PDS). The experimental results showed that N,P-HC, with its large specific surface area (871.73 m2.g-1), high N-pyridine and N-pyrrolic content as well as the existence of P-O-C and O-P-C bonds, provides high degradation performance (98% degradation of Rhodamine B (RhB) in 40 min with a an apparent rate constant (kapp) of 0.055 min-1 and an excellent turnover frequency (TOF) of 0.275 min-1. The quenching study revealed that singlet oxygen generation (1O2) and direct electron transfer were the main reaction ways for the non-radical pathway in the degradation of RhB. The improved catalytic efficiency can be attributed to the synergistic effect created between N and P atoms in the graphitic structure of the carbocatalyst. On the other hand, a heat treatment at 500°C of the used N,P-HC carbocatalyst allows recovery efficiently their performance. Overall, this study provided a facile and clean method for efficiently synthesizing a high-performance N,P co-doped olive pomace-based carbocatalyst for water depollution in presence of PDS.
Current status and demand of “Internet + medical” services by the elderly in China: a cross-sectional study
Authors: Guo Yufei , Cai Yingjie , Huang Chuhan , Tang Xu , Shi Tieying 
Published: September 2022

Abstract
Objective: This paper aims to analyze the current situation and demand of “Internet + medical” services of the elderly in China, and to provide a reference for helping the elderly group to better use “Internet + medical” services.
Methods: A cross-sectional survey was conducted among 434 elderly people using the self-made “Internet + medical” service usage questionnaire and usage demand questionnaire for the elderly.
Results: The utilization rate of “Internet + medical” service was low (31.1%). Age, education level, and personal monthly income are the factors affecting the current use of “Internet + medical”services for the elderly ( P＜0.05). The elderly have a high demand for “Internet + medical” services, with the top three ranking in demand for disease and treatment-related information (M=3.29), knowledge of health and nutrition care (M=3.28), and appointment registration (M=3.24). The relatively low demand was participation in online telemedicine treatment (M=2.71), online telemedicine consultation (M=2.75), and online prescribing (M=2.76).
Conclusion: The elderly in China to “Internet + medical” service utilization rate is low, but the use demand is higher. “Internet + medical” service in the elderly application is affected by a variety of factors. Therefore,we should increase publicity, development of aging operation platform, improve the elderly group to the use of “Internet + medical” service.
How do we monitor degraded areas recovery projects?
Authors: Torres Maria Fernanda Oliveira , Ferreira Robério Anastácio , Mendonça Fernanda Cristina Aguiar , Santos Renan Costa , Silva-Mann Renata 
Published: September 2022

AbstractAreas undergoing forest restoration need to be monitored to achieve ecosystem services. This work was carried out to evaluate the establishment of the riparian forest area in the restoration process, after 18 years of intervention, through temporal analysis of the landscape, biotic and soil indicators. Moreover, a temporal analysis of the landscape, biomass and Gross Primary Production (GPP) was carried out, via geoprocessing, at five-intervals within the period from 2002 to 2020, as well as biotic indicators (seed rain, seed bank, seedlings), and edaphic indicators from 2019 to 2020. The seed rain presented 1,197 propagules, belonging to 27 different species. The soil seed bank showed higher density in the rainy season (21.3 seeds/m2), 25 seeds, seven species, and only one botanical family recorded. In the seedling bank, 1,193 seedlings were reported, belonging to 28 botanical families, in which 57% of the individuals are arboreal-shrubby of the forest. Edaphic conditions improved compared to the initial project implementation. In 2010 using the temporal analysis of the landscape, the planting area was 100% covered with dense vegetation. For biomass and the GPP is a linear increment over time. The successional stage of the area is advanced, with a significant representation of secondary and climax species. The monitoring favored understanding the dynamics of the restoration environment and conservation.