bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure of a G protein-coupled receptor with GRK2 and a biased ligand
Authors: Duan Jia , Liu Heng , Ji Yujie , Yuan Qingning , Li Xinzhu , Wu Kai , Gao Tianyu , Zhu Shengnan , Yin Wanchao , Jiang Yi , Xu H. Eric 
Published: October 2022

AbstractPhosphorylation of G protein-coupled receptors (GPCR) by GPCR kinases (GRKs) desensitizes G protein signaling and promotes arrestin signaling, which is also modulated by biased ligands1–6. Molecular assembly of GRKs to GPCRs and the basis of GRK-mediated biased signaling remain largely unknown due to the weak GPCR-GRK interactions. Here we report the complex structure of neurotensin receptor 1 (NTSR1) bound to GRK2, Gαq, and an arrestin-biased ligand, SBI-5537, at a resolution of 2.92 Å. The high-quality density map reveals the clear arrangement of the intact GRK2 with the receptor, with the N-terminal helix of GRK2 docking into the open cytoplasmic pocket formed by the outward movement of the receptor TM6, analogous of the binding of G protein to the receptor. Strikingly, the arrestin-biased ligand is found at the interface between GRK2 and NTSR1 to enhance GRK2 binding. The binding mode of the biased ligand is compatible with arrestin binding but is clashed with the binding of a G protein, thus provide an unambiguous mechanism for its arrestin-biased signaling capability. Together, our structure provides a solid model for understanding the details of GPCR-GRK interactions and biased signaling.
Polymerization cycle of actin homolog MreB from a Gram-positive bacterium
Authors: Mao Wei , Renner Lars D. , Cornilleau Charlène , Li de la Sierra-Gallay Ines , Benlamara Sarah , Ah-Seng Yoan , Van Tilbeurgh Herman , Nessler Sylvie , Bertin Aurélie , Chastanet Arnaud , Carballido-López Rut 
Published: October 2022

AbstractIn most rod-shaped bacteria, the actin homologue MreB is an essential component of the protein complex effecting cell wall elongation. The polymerization cycle and filament properties of eukaryotic actin have studied for decades and are well characterized. However, purification andin vitrowork on MreB proteins have proven very difficult. Current knowledge of MreB biochemical and polymerization properties remains limited and is based on MreB proteins from Gram-negative species. In this study, we report the first observation of organized filaments and the first 3D-structure of MreB from a Gram-positive bacterium. We have purified MreB from the thermophilicGeobacillus stearothermophilusand shown that it forms straight pairs of protofilamentsin vitro, and that polymerization depends on the presence of both lipids and nucleotide triphosphate. Two spatially close short hydrophobic sequences mediate membrane anchoring. Importantly, we demonstrate that unlike eukaryotic actin, nucleotide hydrolysis is a prerequisite for MreB interaction with the membrane, and that binding to lipids then triggers polymerization. Based on our results, we propose a molecular model for the mechanism of MreB polymerization.
Dual Noncanonical Amino Acid Incorporation Enabling Chemoselective Protein Modification at Two Distinct Sites in Yeast
Authors: Lahiri Priyanka , Martin Meghan S. , Lino Briana R. , Scheck Rebecca A. , Van Deventer James A. 
Published: October 2022

SummaryIncorporation of more than one non-canonical amino acid (ncAA) within a single protein endows the resulting construct with multiple useful features such as augmented molecular recognition or covalent crosslinking capabilities. Herein, for the first time, we demonstrate the incorporation of two chemically distinct ncAAs into proteins biosynthesized inSaccharomyces cerevisiae. To complement ncAA incorporation in response to the amber (TAG) stop codon in yeast, we evaluated opal (TGA) stop codon suppression using three distinct orthogonal translation systems. We observed selective TGA readthrough without detectable cross-reactivity from host translation components. Readthrough efficiency at TGA was modulated by factors including the local nucleotide environment, gene deletions related to the translation process, and the identity of the suppressor tRNA. These observations facilitated systematic investigation of dual ncAA incorporation in both intracellular and yeast-displayed protein constructs, where we observed efficiencies up to 6% of wildtype protein controls. The successful display of doubly-substituted proteins enabled the exploration of two critical applications on the yeast surface - A) antigen-binding functionality; and B) chemoselective modification with two distinct chemical probes through sequential application of two bioorthogonal click chemistry reactions. Lastly, by utilizing a soluble form of a doubly-substituted construct, we validated the dual incorporation system using mass spectrometry and demonstrated the feasibility conducting selective labeling of the two ncAAs sequentially using a ”single-pot” approach. Overall, our work facilitates the addition of a 22ndamino acid to the genetic code of yeast and expands the scope of applications of ncAAs for basic biological research and drug discovery.Graphical AbstractHerein we report the establishment of dual noncanonical amino acid incorporation in yeast to support expression and site-selective labelling of doubly substituted proteins in solution and on the yeast surface.
Structural basis of V-ATPase VOregion assembly by Vma12p, 21p, and 22p
Authors: Wang Hanlin , Bueler Stephanie A. , Rubinstein John L. 
Published: October 2022

AbstractVacuolar-type ATPases (V-ATPases) are rotary proton pumps that acidify specific intracellular compartments in almost all eukaryotic cells. These multi-subunit enzymes consist of a soluble catalytic V1region and a membrane-embedded proton-translocating VOregion. VOis assembled in the endoplasmic reticulum (ER) membrane and V1is assembled in the cytosol. However, V1binds VOonly after VOis transported to the Golgi membrane, thereby preventing acidification of the ER. We isolated VOcomplexes and subcomplexes fromSaccharomyces cerevisiaebound to V-ATPase assembly factors Vma12p, Vma21p, and Vma22p. Electron cryomicroscopy shows how the Vma12-22p complex recruits subunits a, e, and f to the rotor ring of VOwhile blocking premature binding of V1. Vma21p, which contains an ER-retrieval motif, binds the VO:Vma12-22p complex, ‘mature’ VO, and a complex that appears to contain a ring of loosely-packed rotor subunits and the proteins YAR027W and YAR028W. The structures suggest that Vma21p binds assembly intermediates that contain a rotor ring, and that activation of proton pumping following assembly of V1with VOremoves Vma21p, allowing V-ATPase to remain in the Golgi. Together, these structures show how Vma12-22p and Vma21p function in V-ATPase assembly and quality control, ensuring the enzyme acidifies only its intended cellular targets.
Love or like: gender effects in emotional expression in online reviews
Authors: Abdul-Ghani Eathar , Kim Jungkeun , Kwon Junbum , Hyde Kenneth F. , Cui Yuanyuan (Gina) 
Published: October 2022

Purpose
Given the socialisation of men and women to their gender roles and expression of emotion, this study aims to investigate whether there are gender differences in the use of emotive language in electronic word-of-mouth (eWOM), specifically in online reviews. The authors propose that female reviewers will use strong emotive terms, such as love, more frequently in online reviews than do male reviewers. The authors further propose that the gender of the reviewer influences audience responses to the reviewer’s use of emotive terms in online reviews.

Design/methodology/approach
The authors conducted secondary data analysis of restaurant reviews (Study 1) to provide evidence on whether the gender of the reviewer affects the frequency of use of emotive terms in an online review. In addition, three separate experiments (Studies 2–4) were conducted to test the theoretical arguments.

Findings
The results of the secondary data analysis indicated that female online reviewers used the term “love” much more frequently in their reviews than male reviewers, whereas there was no usage difference for the term “like”. The experimental studies further showed that an emotive review by a male reviewer containing the word “love” resulted in a higher evaluation of the restaurant being reviewed than a non-emotive review containing the word “like”. This difference was stronger when the overall rating was less salient and for consumers who believe (vs do not believe) that men and women use emotional language differently.

Research limitations/implications
First, the paper extends our understanding of gender differences in emotional expression within the domain of eWOM and online reviews as well as our understanding of consumer responses to these gender differences. Second, the authors identify a boundary condition for these gender effects, namely, the overall rating score. Third, the authors find that consumer beliefs regarding gender stereotypes in emotional expression provide an explanation for these effects.

Practical implications
The results of the research indicate that the electronic algorithms operating on review sites might be modified in terms of their criteria for selecting the reviews to display to consumers, as consumer decision-makers may find greater utility in reviews written by male reviewers that contain strongly positive emotive terms.

Originality/value
The research extends the knowledge on gender differences in emotional expression in online reviews by demonstrating the actual usage patterns and differing responses to the emotional expressions of each gender.
Embedding sustainability in university work experience placements: a De Montfort University model
Authors: Onyido Tochukwu Ben C. , Allman Zoe , Hardaker Pamela , Rughani Deepa , Letinov Allan 
Published: October 2022

PurposeThe paper looks at the feasibility of university placements supporting small and medium-scale enterprises (SMEs) to operate in a sustainable manner. Due to size and resource constraints, many SMEs may regard sustainability more as a burden than a value-adding commercial strategy.Design/methodology/approachA two-year study was conducted on the sustainability-themed placements of 101 students of De Montfort University, United Kingdom, in SMEs. The placements were designed with the purpose of acting as a traditional work experience scheme while also equipping students with learning, skills and orientation with which to act as sustainability champions within companies. The study combined the use of students' reflections (via tools such as monthly logbooks) with interviews and questionnaire surveys of both employers and students, in order to evaluate the outcomes of the placements.FindingsStudents engaged with the sustainability aspect of their placements mainly by obtaining information on the sustainability performance of SMEs, with significant engagement also occurring in the areas of sustainability advocacy (e.g. proposing socio-environmental plans to companies) and initiatives/action.Practical implicationsPlacements can potentially serve as a means of knowledge generation for universities while providing SMEs with cost-effective staff and innovation sustainability resources.Originality/valueA circular approach to university placement programmes is proposed whereby the knowledge gained from previous placements about SMEs' sustainability performance is used to prepare later cohorts of placement students with a pragmatic understanding of challenges and opportunities related to the implementation of sustainability by SMEs.
Female superiority in accruals quality: some evidence from the Scandinavian region
Authors: Mnif Yosra , Cherif Imen 
Published: October 2022

Purpose
The purpose of this paper is twofold. First, this study examines the relationship between the presence of a female (rather than a male) audit partner and the client firm’s accruals quality. Second, this study explores whether and how the female audit partners’ specific attributes influence the gendered auditor effect on the quality of the client firm’s accruals quality (if it exists).

Design/methodology/approach
Research hypotheses have been tested by conducting both univariate and multivariate empirical analyses based on a large sample of firm-year observations from the Swedish Corporation for the years 2010–2019. During the sample timeframe, the client firms have been audited by 56 female and 231 male audit partners.

Findings
The research findings first indicate that client firms of female audit partners are associated with downward earnings management, indicating a beneficial female auditor effect on client firm’s accruals quality. Results from the audit partner change analysis exhibit that the adverse female auditor effect on the client firm’s earnings management, and hence, the beneficial female auditor effect on the client firm’s accruals quality occurs from the first year of the assignment of a female audit partner to replace a male audit partner. When looking at how specific attributes of female audit partners influence accruals quality of their audited clients, this study reveals that the favorable female auditor effect on the client firm’s accruals quality holds constant for all the female audit partners’ specific attributes included in the researched models. This underscores that the mere presence of a female audit partner constrains earnings management and enhances, thereby, the client firm’s accruals quality.

Originality/value
This research supports regulators calling for the appointment of more women to the audit firms’ leading ranks (e.g. leadership).
The declining GAAP ETR trend over 1960-2016
Authors: Lu Yuzhu , Shao Liang , Zhang Yue 
Published: October 2022

Purpose
This study aims to provide a comprehensive analysis on the reasons of the observed trend in the GAAP ETR over 1960–2016.

Design/methodology/approach
The authors use a linear tax function which allows for time-varying coefficients to track the trend in GAAP ETR over 1960–2016. This approach can decompose the ETR trend into the trends of the statutory tax rate, the propensity to recognize taxes, the tax-related firm characteristics and their coefficients. Thus, the authors can quantify the contribution of each factor in the tax function to the ETR trend.

Findings
Before 1988, the declining trend in tax expense is mainly driven by changes in the statutory tax rate; in contrast, after 1988, the trend is completely explained by firms’ decreasing propensity to recognize tax expense. While prevalent across different groups of firms, the decreasing propensity to recognize tax expense in the recent 30 years is more pronounced among firms that have higher needs for tax savings or greater tax-saving advantages.

Originality/value
To the best of the authors’ knowledge, this study is the first one that uses a trend analysis to examine the reasons for the downward trend in tax expense over a long period (1960–2016). The results show that, although the trend appears for the full sample period, it is driven by different forces between the first and second half of the time window. A decreasing propensity to recognize tax expense is the main reason for only the trend in recent years, which calls for attention from academia and policymakers. The results also show which firms have had faster trends in their propensity to recognize tax expense, suggesting targets for tax enforcement and tax researchers.
Investigating the determinants of using clothing subscription rental services: a perspective from Chinese young consumers
Authors: Myin Mon Thu , Su Jin , Wu Huicheng (Jeff) , Shen Haina 
Published: October 2022

Purpose
The purpose of this study is to investigate the impacts of Chinese young consumers’ traits (fashion leadership, quality consciousness, price consciousness, environmental consciousness and social media usage) as the external factors on their behavioral intention toward using clothing subscription rental services.

Design/methodology/approach
Quantitative research was conducted, and empirical data were collected from 255 Chinese college students. Structural equation modeling was conducted to test the proposed hypotheses.

Findings
The study offered convincing evidence that fashion leadership, price consciousness and social media usage are the three main drivers of Chinese young consumers’ favorable attitudes toward clothing subscription rental services, which together with social norms further lead to their intention to use clothing subscription rental services. Contrary to expectations, the study showed that consumers’ quality consciousness and environmental consciousness have no significant impact on consumer attitudes toward clothing subscription rental services. Moreover, the findings of the study demonstrated the impact of attitude and subjective norms on Chinese young consumers’ intention to use clothing subscription rental services.

Originality/value
This study contributes to the literature by integrating additional factors (fashion leadership, quality consciousness, price consciousness, consumer environmental knowledge, social media usage) into the traditional theory of reasoned action model to investigate how Chinese young consumers’ characteristics impact their attitudes and how their attitudes and subjective norms impact their intention toward using clothing subscription rental services.
A Chinese version of the revised Fibromyalgia Impact Questionnaire: A validation study
Authors: Li Yan‐ting , Jiang Quan , Jia Yuan , Zhang Yong‐feng , Xiao Yu‐ya , Wang Hui , Zhao Ya‐yun , Jiao Juan 
Published: October 2022

AbstractObjectiveThe revised Fibromyalgia Impact Questionnaire (FIQR) was developed to measure the quality of life of patients with fibromyalgia in randomized controlled trials and routine care. The purpose of this study was to translate and adapt the FIQR from English to Chinese, and to examine the validity and reliability of the Chinese version of the FIQR (CFIQR).MethodsFollowing the translation of the FIQR, fibromyalgia patients from 6 centers were recruited and completed the CFIQR, the validated Chinese version of the Medical Outcome Study Short Form 36 Health Survey (SF‐36) and the Beck Depression Inventory (BDI). In this study, Cronbach's alpha coefficient, test–retest reliability and item total correlation were used for evaluating external and internal reliability; and criterion and structural validity were evaluated.ResultsA total of 200 fibromyalgia patients completed the study. The internal consistency was excellent (Cronbach's alpha .90, .88, .88 and .93 for function, overall impact, symptoms scales and total score, respectively; item‐total correlations from .25 to .83.) Test–retest reliability levels of the CFIQR total and subscale scores were strong correlation (intraclass correlation coefficient >0.75). Furthermore, there were significant correlations between the 3 subscale and the total score of the CFIQR and the SF‐36, as well as the CFIQR and the BDI, by criterion validity (P < .01). Confirmatory factor analysis gave an acceptable value for structural validity according to the 3‐factor structures of function, overall impact and symptoms.ConclusionsThe CFIQR is a valid and reliable instrument for both clinical practice and research purposes with Chinese speakers globally. [ClinicalTrials.gov: NCT03381131].
Intraoperative ultrasound: “Alternative eye” in lymph nodal dissection in non‐small cell lung cancer
Authors: Messina Gaetana , Bove Mary , Noro Antonio , Opromolla Giorgia , Natale Giovanni , Mirra Rosa , Capasso Francesca , Pica Davide Gerardo , Di Filippo Vincenzo , Pirozzi Mario , Caterino Marianna , Facchini Sergio , Zotta Alessia , Polito Rita , Vicidomini Giovanni , Santini Mario , Fiorelli Alfonso , Ciardiello Fortunato , Fasano Morena 
Published: October 2022

AbstractIntroductionStaging of the mediastinum lymph nodes involvement in patients with non–small cell lung cancer (NSCLC) is an important prognostic factor determining the most appropriate multimodality treatment plan. The objective of this study is to assess ultrasound characteristics of mediastinal lymph nodes metastasis and effectiveness of intraoperative ultrasound‐guided mediastinal nodal dissection in patients with resected NSCLC.Materials and MethodsAll patients undergoing video‐assisted thoracoscopic surgery lobectomy and pulmonary lymphadenectomy from November 2020 to March 2022 at the thoracic surgery department of the Vanvitelli University of Naples underwent intraoperative ultrasound‐guided mediastinal lymph nodal dissection.ResultsThis study evaluates whether individual B‐mode features and a compounding thereof can be used to accurately and reproducibly predict lymph node malignancy.DiscussionIntraoperative ultrasound, during systematic mediastinal lymph node dissection, is helpful in preventing lesion to mediastinal structures. Pathological nodal sonographic characteristics are round shape, short‐axis diameter, echogenicity, margin, the absence or presence of coagulation necrosis sign, and the absence or presence of central hilar structure, increased color Doppler flow, the absence or presence of calcification, and nodal conglomeration. Operating time was not substantially prolonged. The procedure is simple, safe and highly accurate.ConclusionsUltrasonic techniques allow surgeons to detect the relationship between lymph nodes and surrounding large blood vessels during biopsy, improving the safety and simplicity of the operation, increasing the number of harvested lymph nodes, and reducing the risk of intraoperative injury; it is a fast, easily reproducible, and inexpensive method.
Morphometric analysis of the skull of Hamdani sheep via Three‐Dimensional modelling
Authors: Dayan Mustafa Orhun , Demircioğlu İsmail , Koçyiğit Ali , Güzel Barış Can , Karaavci Funda Aksünger 
Published: October 2022

AbstractThe aim of the present study was to determine the craniometric characteristics of the crania of Hamdani sheep, one of local breeds of Turkey, by using computed tomography (CT). In the study, 13 (six female and seven male) skulls were used. After CT images of the skulls were taken, their three‐dimensional models were created by MIMICS 20.1 (The Materialise Group, Leuven, Belgium) program. Thirty‐eight craniometric measurements were taken over the models created and nine indices were calculated by using these measurements. All the characteristics examined were expressed as mean ± SE. In the study, statistically significant differences were found between females and males in terms of greatest neurocranium breadth, facial breadth, greatest palatal breadth (p < 0.05) and height of the foramen magnum (p < 0.01) parameters. No statistically significant difference was observed between the sexes in the calculated index values (p > 0.05). In conclusion, it is thought that the findings obtained from the study will contribute to the zooarchaeology and sheep taxonomy of Mesopotamian region.