bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of leisure and sports consumption and sports motivation among children with disabilities
Authors: Laoues-Czimbalmos Nóra , Szerdahelyi Zoltán , Müller Anetta 
Published: February 2022

The number of studies on the leisure and sporting habits of people with disabilities as well as the background of sports motivation is far from the focus of research on intact research. Thus, the assessment of physical habits and sports motivation factors determining the quality of life of persons with disabilities can be considered as a less researched deficit area in Hungary and internationally.

In our research, we examine the recreational and sporting habits of the 8-18 year-olds, as well as the motivational background, and the results of this research are presented in this paper. The importance of this issue is confirmed by the fact that, according to a UN survey, 650 million people have some kind of disability, 50 million of them in Europe and nearly half a million in Hungary, 5% of the population. The questionnaire (N = 639) was filled by children with disabilities living in the North Great Plain region with the help of a teacher. We used both closed and open questions in the questionnaire, and we helped and orient children with response categories for some questions.

Data were processed using SPSS software, averaged, standard deviation, median, mode, and Chi2 probe was used to examine relationships in addition to basic statistics.

Our results showed that it is a problem for young people with disabilities that they prefer passive leisure activities, which is similar to the results of studies on intact research. Boys and girls have different sports, boys are more active than girls.
HFE Gene Regulation of T Lymphocyte Activity Can Be a Potential Target of PCOS
Authors: Song Yu-Hang , Chen Ya-Nan , Wang Bin-Lu , Du Guo-Qing , Li Yao , Xu Qi 
Published: February 2022

Abstract
Background Polycystic ovary syndrome (PCOS) may be characterized by skeletal muscle abnormalities. Pioglitazone has a certain therapeutic effect on skeletal muscle metabolism in PCOS patients.Objective To investigate the other effects of pioglitazone on skeletal muscle and its mechanism.Methods We used weighted gene co-expression network analysis (WGCNA) to identify potential key genes and signaling pathways involved in pioglitazone affecting skeletal muscle in PCOS patients. First, download the chip data of GSE8157 from GEO database. Then, modules related to genes expressed in skeletal muscle of PCOS patients before and after pioglitazone treatment were screened by WGCNA, and core genes and differential gene analysis methods were further selected according to the correlation characteristics between modules and clinical manifestations. Finally, the selected genes were enriched for analysis.Results Four significantly up-regulated genes were screened out by differential gene analysis: MTBP, MAPK14, RBBP6 and PTPRC. They are both associated with cell cycle regulation and immune cell (T cell) antigen receptor signaling.Conclusions The effect of pioglitazone on skeletal muscle of PCOS patients is mainly reflected in the regulation of abnormal expression of cell cycle and immune cell-related genes. Among them, HFE gene has the most obvious regulation effect on T lymphocyte activity, which may be a potential gene target for the treatment of PCOS.
Impact of A Peer-Led, Community-Based Parenting Programme Delivered At A National Scale: An Uncontrolled Cohort Design With Benchmarking
Authors: Day Crispin , Harwood Joshua , Kendall Nadine , Nicoll Jo 
Published: February 2022

Abstract
Background: Childhood behavioural are the most common mental health disorder worldwide and represent a major public health concern, particularly in socially disadvantaged communities. Treatment barriers mean that up to 70% of children do not receive recommended parenting interventions. Innovative approaches, including evidence-based peer-led models, such as Empowering Parents Empowering Communities’ (EPEC) Being a Parent (BAP) programme, have the potential to reduce childhood difficulties and improve parenting if replicable and successfully delivered at scale. Method: This real-world quasi-experimental study, with embedded RCT benchmarking, examined the population reach, attendance, acceptability and outcomes of 128 BAP groups (n=930 parents) delivered by 15 newly established sites participating in a UK EPEC scaling programme. Results: Scaling programme (SP) sites successfully reached parents living in areas of greater social deprivation (n=476, 75.3%), experiencing significant disadvantage (45.0% left school by 16; 39.9% lived in rental accommodation; 36.9% lone parents). The only benchmarked demographic difference was ethnicity, reflecting the greater proportion of White British parents living in scaling site areas (SP 67.9%; RCT 22.4%). Benchmark comparisons showed scaling sites’ parent group leaders achieved similar levels of satisfaction. Scaling site parent participants reported substantial levels of improvement in child concerns (ES 0.6), parenting (ES 0.9), parenting goals (ES 1.2) and parent wellbeing (ES 0.6) that were of similar magnitude to RCT benchmarked results. Though large, parents reported lower levels of parenting knowledge and confidence acquisition compared with the RCT benchmark. Conclusion: Despite common methodological limitations associated with real-world scaling evaluations, findings suggest that this peer-led, community-based, parenting approaches can be replicated at scale and may have considerable potential to improve child and parenting difficulties, particularly for socially disadvantaged populations.
Climate change farm based autonomous adaptation measures and its impact on wheat crop productivity in Punjab, Pakistan
Authors: Ahmad Dilshad , Afzal Muhammad 
Published: February 2022

Abstract
Climate induced hazards has increased production threats to agriculture where such environmental risks can significantly reduced by adapting adaptation measures. In farm based adaptation measures specifically the autonomous adaptation aspect has not properly focused particularly in developing countries like Pakistan. This study attempted to investigate on-farm autonomous adaptation (OFAA) measures of wheat farmers to climate change and estimate its impact on wheat yield and total return. This research work used the data of 480 wheat farmer from production based six categorized higher vulnerable districts of Punjab, Pakistan. Probit model was employed to examine adaptation decisions determinants of farmers and Propensity Score Matching used for to investigate the impact of OFAA practices on outcomes of wheat crop. Estimates indicated as wheat farmers to reduce the unfavorable effects of climate change have applied different OFAA strategies such as seed varieties changing, fertilizer management, variations in cultivation dates and management of supplementary irrigated water. Probit model estimates illustrated as climate information access, credit access, ownership of land, off-farm income and ownership of tubewell considered significant determinants regarding adaptation decisions of farmers. Estimates of propensity score matching indicated as farmers those applied almost one OFAA strategy obtained 310kg per hectares higher wheat yield as US$82.75 per hectares return rather than those not applied any adaptation strategies. Adapter wheat farmers applied multiple adaptation strategies obtained higher yield and return rather than adapter farmers used limited adaptation strategies. Results imply as in developing sustainable farmers livelihood, adequate food production, reducing crops variability and climate change adverse impacts it is mandatory to applications of OFAA measures. The study also elaborated as for developing farm-level adaptation there is significant role of climate information, farmers schooling, access of credit and adequate irrigation.
Resilience To Suicidal Behaviour In Young Adults: A Cross-Sectional Study
Authors: Han Jin , Wong Iana , Christensen Helen , Batterham Philip J 
Published: February 2022

Abstract
Despite decades of research on suicide risk factors in young people, there has been no significant improvement in our understanding of this phenomenon. This study adopts a positive deviance approach to identify the individuals with suicide resilience and to describe their associated psychological and sociodemographic profiles. Australian young adults aged 18 to 25 years with suicidal thoughts (N=557) completed an online survey covering sociodemographic, mental health status, emotion regulatory and suicide-related domains. Latent class analysis was used to identify the individuals with suicide resilience. Predictors of suicide resilience were assessed using logistic regression models. Results suggested that one in ten (n=55) met criteria for suicide resilience. Factors that had a significant association with suicide resilience included greater cognitive flexibility, greater self-efficacy in expressing positive affect, reduced use of digital technology and less self-harm and substance use as a response to emotional distress. This study identified the factors that may protect young adults with suicidal thoughts from progressing to suicide attempts. Suicide prevention programs might be optimised by shifting from a deficit-based to a strength-based approach through promoting cognitive flexibility, self-efficacy and reducing maladaptive coping.
Identification of CaAN3 as a fruit-specific regulator of anthocyanin biosynthesis in pepper (Capsicum annuum)
Authors: Byun Jinyoung , Kim Tae-Gun , Lee Joung-Ho , Li Ning , Jung Soyoung , Kang Byoung-Cheorl 
Published: February 2022

Abstract
The key regulatory gene CaAN2 encodes an R2R3 MYB transcription factor that regulates anthocyanin biosynthesis in various tissues in pepper ( Capsicum annuum ). However, CaAN2 is not expressed in certain pepper accessions showing fruit-specific anthocyanin accumulation. In this study, we identified the novel locus CaAN3 as a regulator of fruit-specific anthocyanin biosynthesis, using an F 2 population derived from a hybrid cultivar with purple immature fruits and segregating for CaAN3 . We extracted total RNA, assembled two RNA pools according to fruit color, and carried out bulked segregant RNA sequencing. We aligned the raw reads to the pepper reference genome Dempsey and identified 6,672 significant single nucleotide polymorphisms (SNPs) by calculating the Δ(SNP-index) between the two pools. We then conducted molecular mapping to delimit the target region of CaAN3 to the interval 184.6–186.4 Mbp on chromosome 10. We focused on Dem.v1.00043895, encoding an R2R3 MYB transcription factor, as the strongest candidate gene. Sequence analysis revealed four insertion/deletion polymorphisms in the promoter region of the green CaAN3 allele. We employed virus-induced gene silencing and transient overexpression assays to characterize the function of the candidate gene. When Dem.v1.00043895 was silenced in pepper, anthocyanin accumulation decreased in the pericarp, while the transient overexpression of Dem.v1.00043895 in Nicotiana benthamiana leaves resulted in the accumulation of anthocyanins around the infiltration sites. These results showed that Dem.v1.00043895 is CaAN3 , an activator of anthocyanin biosynthesis in pepper fruits.
Association Between Blood Urea Nitrogen to Creatinine Ratio and in-hospital Mortality Among Patients With Acute Myocardial Infarction- A Retrospective Cohort Study
Authors: Huang Sulan , Guo Ning , Duan Xiangjie , Zhou Quan , Zhang Zhixiang , Luo Li , Ge Liangqing 
Published: February 2022

Abstract
Purpose: This study aimed to determine the association between blood urea nitrogen (BUN) and creatinine(Cr) ratio and in-hospital mortality in patients with acute myocardial infarction(AMI).Patients and Methods: This retrospective cohort study included adult patients (≥18 years of age) who were admitted to the intensive care unit (ICU) with a primary diagnosis of AMI were enrolled. Their data were collected from the eICU Collaborative Research Database (eICU-CRD) throughout the continental USA. The data collected from the database included demographic data, vital signs, laboratory test data, and comorbidities. The clinical endpoint was in-hospital mortality. The Cox proportional hazards model was used to evaluate the prognostic values of the basic BUN/Cr ratio, and the Kaplan–Meier method was used to plot survival curves. Subgroup analyses were performed to measure mortality across various subgroups.Results: In total, 3,831 eligible patients were included. In the multivariate analysis, after being adjusted for age, sex, and ethnicity, the BUN/Cr ratio was found to be a significant risk predictor of in-hospital mortality. The nonlinear relationship between the BUN/Cr ratio and in-hospital mortality was explored in a dose-dependent manner with an apparent inflection point of 18. Furthermore, after adjusting for more confounding factors, the BUN/Cr ratio remained a significant predictor of in-hospital mortality (tertile 3 vs.tertile 1: hazard ratio 2.14; 95% confidence interval 1.16-3.97; p<0.05).The Kaplan–Meier curve for tertiles of the BUN/Cr ratio indicated that survival rates were highest and lowest when the BUN/Cr ratio was ≤15.12 and ≥19.41, respectively, after adjustment for age, sex, and ethnicity(p<0.001).Conclusion: Our findings showed that a higher BUN/Cr ratio was associated with an increased risk of in-hospital mortality in patients with AMI. These results support a revision of how we predict the prognosis of patients with AMI.
Differential Gene Expression and Hallmarks of Stemness in Epithelial Cells of the Developing Rat Epididymis
Authors: Dufresne Julie , Gregory Mary , Pinel Laurie , Cyr Daniel 
Published: February 2022

Abstract
Epididymal development can be subdivided into three phases: undifferentiated, a period of expansion, and a period of differentiation. The objectives of this study were: (1) to assess gene expression profiles in epididymides; (2) predict signalling pathways and (3) develop a novel 3D cell culture method to assess the regulation of epididymal development in vitro. Microarray analyses indicate that the largest changes in differential gene expression occurred between the 7 to 18-day period, in which 1452 genes were differentially expressed; while 671 differentially expressed genes were noted between day 18 and 28, and there were 560 differentially expressed genes between days 28 and 60. Multiple signalling pathways were predicted at different phases of development. Pathway associations indicated that in epididymides of 7 to 18-day old rats. there was a significant association of regulated genes implicated in stem cells, estrogens, thyroid hormones, and kidney development, while androgen- and estrogen-related pathways were enriched at other phases of development. Organoids were derived from CD49f+ columnar cells from 7-day old rats, while no organoids developed from CD49f- cells. Cells cultured in an epididymal basal cell organoid medium versus a commercial kidney differentiation medium supplemented with DHT revealed that irrespective of the culture medium, cells within differentiating organoids expressed p63, AQP9 and V-ATPase after 14 days of culture. The commercial kidney medium resulted in an increase in the number of organoids positive for p63, AQP9, and V-ATPase. Together, these data indicate that columnar cells represent an epididymal stem/progenitor cell population.
Evaluation On The Safety of Anti-Platelet Agents In Patients With Acute Pancreatitis: A Retrospective Cohort Study
Authors: Xu Yin , Dong Jun , Fang Junling , Shen Yuhang , Chunfu Zhu 
Published: February 2022

Abstract
Background: Anti-platelet drug has not been paid much attention in guidelines for the treatment of acute pancreatitis, especially in patients with cardiovascular disease and receiving anti-platelet therapy, and its safety hasn’t been evaluated by any guidelines or studies. Methods: A total of 904 patients with acute pancreatitis were enrolled in this cohort study, they were divided into Anti-platelet therapy group (n=297) and non-Anti-platelet therapy group (n=607). The primary result was the mortality in the 14th-day, 28th-day, and 90th-day. Adverse events during treatment included septic shock, respiratory tract infection, urinary tract infection, abdominal infection, bleeding, kidney damage, respiratory distress, cardiac dysfunction, liver dysfunction, and renal dysfunction. Kaplan-meier, propensity score matching (PSM), subsequently, cox regression and subgroup analysis were performed. Results: The mortality rates were 9.55% vs 4.71%, P =0.012,13.34% vs 8.1%, P =0.02,16.14% vs 13.13%, P =0.235, respectively, during the 14-day, 28-day, and 90-day follow-up periods. Risk ratio for death among anticoagulants versus non-anticoagulants after multivariate adjustment (HR=2.815,2.819, 95%CI:1.021-4.667,1.045-4.583, p < 0.05). According to the survival curves, there were significant differences in mortality after 14, 28, and 90 days between the anticoagulant and non-anticoagulant groups (log-rank P<0.05). After PSM, 297 pairs were identified, and there was no significant difference in the risk of death between the anticoagulant and non-anticoagulant groups (all P >0.05). Conclusions: Anti-platelet therapy is not superior to non-prophylactic anti-platelet therapy in terms of reducing mortality and adverse events in acute pancreatitis, and there is no statistically significant difference in mortality and adverse events in the cohort of cardiovascular patients using anti-platelet drugs compared with those without using anti-platelet drugs.
Chlamydia trachomatis enhances HPV persistence through immune modulation
Authors: Lu Yingying , Wu Qi , Wang Li , Ji Lingting 
Published: February 2022

Abstract
Objective: To investigate the immune mechanism of Chlamydia trachomatis (CT) in enhancing the persistent infection of HPV. Methods: 37 CT/HPV coinfections, 53 HPV infections, 30 CT infections and 30 healthy individuals were enrolled. Antigen-presenting ability, LC density, PI3K and MAPK signaling cascades were detected. Flow cytometry was used to determine the surface activation markers on LC, T cell subgroups and apoptosis.Results: The antigen presentation ability and LC density in the CT/HPV coinfection were significantly lower than those in HPV and CT infections, and those in the HPV infection group were significantly lower than control (P<0.05). The PI3K signaling cascade was further activated, and MAPK pathways were reduced in the CT/HPV coinfection compared with the HPV and CT infection groups. There was less CD4+ T cell, more Treg cell in CT/HPV coinfection than HPV infection group (P<0.05); CD8+ T cell becomes fewer but without significance; the CD4+/CD8+ ratio decreased and T cell apoptosis increased in the CT/HPV coinfection group. Conclusion: CT infection could enhance HPV persistance by inhibiting LC functions, including dysregulating the PI3K and MAPK pathways and reducing LC numbers. CT could also alter T cell subsets and apoptosis, thus impairing cell-mediated immunity and accelerating the progression of cervical cancer.
Software Validation: Sisped (Diabetic Foot System) As A Diagnostic Support
Authors: Diógenes Ligiane Medeiros , Malerbi Domingos Augusto , Melo Naira Horta , Rocha Roseanne Montargil , de Almeida-Pititto Bianca , Melo Karla , Firmino Carlos Menandro de Lima , Malerbi Fani Eta Korn , Santos Elenalda Ferreira dos , Andrade Leila Maciel , Rocha Thaisa de Fátima Almeida , Santos Leticia Adrielle dos , Rezende Karla Freire , Parizi Maria Cândida Ribeiro 
Published: February 2022

Abstract
Introduction: Diabetic foot is responsible for 40% to 60% of all non-traumatic amputations. Early and intensive ulcer identification and treatment have been shown to reduce the number of amputations by 45 to 90%. Due to the deficit of low-cost computerized programs and aiming at an improvement in the prevention of diabetic foot complications, the Diabetic Foot System-SISPED was created. Therefore, the present work aims to validate the applicability of this SISPED system. Methods: 117 clinical foot examination records performed by a team of specialists in the detection of the risk foot were evaluated and lauded. Cases were categorized as having or not a risk foot, and the latter, when diagnosed, was classified as described in the literature. These data were then entered into SISPED. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of the reports were calculated. Results: The mean age of the participants was 55.3 ± 15.5 years, female gender was predominant (71.8%), the meantime of Diabetes Mellitus (DM) diagnosis was 11.9 ± 9.6 years, and the mean glycated hemoglobin was 8.5±1.9%. Patients had type 1 risk foot in 85.5% of cases and only 12% had normal feet. Analysis of the SISPED results indicated a sensitivity of 92.8%, specificity of 99%, positive predictive value (PPV) of 92.8%, negative predictive value (NPV) of 99%, and accuracy of 98.3%. Conclusion: Comparison of the results of SISPED reports, which should be used by general practitioners, with those of a professional specialized in diabetic foot showed equivalence in diagnostic accuracy. The SISPED was valid to be implemented in the Brazil’s National Health System (SUS), in Sergipe, as a diagnostic support tool for health professionals in the assistance to people with diabetes mellitus.
Direct Effects of Elevated Dissolved CO2 Can Alter the Population Dynamics of Freshwater Zooplankton
Authors: Ramaekers Lana , Pinceel Tom , Brendonck Luc , Vanschoenwinkel Bram 
Published: February 2022

Abstract
Dissolved CO2 levels (pCO2) are increasing in lentic freshwaters across the globe. Recent studies have shown that this will impact the nutritional quality of phytoplankton as primary producers, however, the extent to which freshwater zooplankton may also be directly affected remains unclear. We test this in three model species representative of main functional groups of primary consumers in freshwaters; the water flea Daphnia magna, the seed shrimp Heterocypris incongruens and the rotifer Brachionus calyciflorus. We experimentally exposed individuals to three pCO2 levels (1,500; 25,500 and 83,000 ppm) to monitor changes in life history in response to current, elevated and extreme future pCO2 conditions in ponds and shallow lakes. All species had reduced survival under the extreme pCO2 treatment, but the water flea was most sensitive. Body size and reproduction were reduced at 25,500 ppm in the water flea and the seed shrimp and population growth was delayed in the rotifer. Overall, our results show that direct effects of pCO2 could impact the population dynamics of freshwater zooplankton. By differentially modulating the life history of functional groups of primary consumers, elevated pCO2 has the potential to change the evolutionary trajectories of populations as well as the ecological functioning of freshwater communities.