bluuebunny/crossref_metadata_2025_split huggingface dataset:
An energy‐efficient auto clustering framework for enlarging quality of service in Internet of Things‐enabled wireless sensor networks using fuzzy logic system
Authors: Padmanaban Paruthi Ilam Vazhuthi , Shanmugaperumal Periasamy Manikandan , Aruchamy Prasanth 
Published: August 2022

SummaryThe advancement of the Internet of Things (IoT) technologies will play a vital role in the evolution of the smart city, smart healthcare, and smart grid applications. Wireless sensor network (WSN) is one of the futuristic technologies utilized for sensing and data exchange processes in IoT‐enabled applications. However, the hotspot problem, control packet overhead, and inefficient clustering are the most significant challenges to attaining an energy‐efficient IoT‐enabled WSN (IWSN) model. Most of the existing clustering schemes lagged to mitigate these aforesaid constraints since it consumes extra energy for data computation and forwarding tasks under any environmental conditions. In this article, a novel energy‐efficient auto clustering (EEAC) framework has been proposed to develop an effective IWSN model with enhanced quality of service. The EEAC framework comprises three phases such as zone formation, node classification, and auto clustering phases. The objective of the first phase is to significantly form the different zones by alleviating the hotspot problem. Subsequently, the fuzzy logic algorithm is employed in the second phase to classify the nodes as master, sub‐master, and member nodes. Finally, the third phase of the proposed framework will accomplish the auto clustering mechanism based on hop information received from the reported packet. The performance results evident that the proposed EEAC framework obtains a lesser energy consumption of 0.01 J during a dense network and the network lifetime is prolonged up to 52% when compared with existing state‐of‐the‐art clustering models.
Efficient Cas9‐based Genome Editing Using CRISPR Analysis Webtools in Severe Early‐onset‐obesity Patient‐derived iPSCs
Authors: Patel Achchhe , Iannello Grazia , Diaz Alejandro Garcia , Sirabella Dario , Thaker Vidhu , Corneo Barbara 
Published: August 2022

AbstractThe CRISPR system is an adaptive defense mechanism used by bacteria and archaea against viruses and plasmids. The discovery of the CRISPR‐associated protein Cas9 and its RNA‐guided cleavage mechanism marked the beginning of a new era in genomic engineering by enabling the editing of a target region in the genome. Gene‐edited cells or mice can be used as models for understanding human diseases. Given its high impact in functional genomic experiments on different model systems, several CRISPR/Cas9 protocols have been generated in the past years. The technique uses a straightforward “cut and stitch” mechanism, but requires an accurate step‐by‐step design. One of the key points is the use of an efficient programmable guide RNA to increase the rate of success in obtaining gene‐specific edited clones. Here, we describe an efficient editing protocol using a ribonucleotide protein (RNP) complex for homology‐directed repair (HDR)–based correction of a point mutation in an induced pluripotent stem cell (iPSC) line generated from a 14‐year‐old patient with severe early‐onset obesity carrying a de novo variant of ARNT2. The resulting isogenic iPSC line, named CUIMCi003‐A‐1, has a normal karyotype, expresses stemness markers, and can be differentiated into progenies from all three germ layers. We provide a detailed workflow for designing a single guide RNA and donor DNA, and for isolating clonal human iPSCs edited with the desired modification. This article also focuses on parameters to consider when selecting reagents for CRISPR/Cas9 gene editing after testing their efficiency with in silico tools. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.This article was corrected on 23 January 2023. See the end of the full text for details.Basic Protocol 1: Design of sgRNAs and PCR primersBasic Protocol 2: Testing the efficiency of sgRNAsBasic Protocol 3: Design of template or donor DNABasic Protocol 4: Targeted gene editingBasic Protocol 5: Selection of positive clonesBasic Protocol 6: Freezing, thawing, and expansion of cellsBasic Protocol 7: Characterization of edited cell lines
Use of hyaluronic acid for regeneration of maxillofacial bones
Authors: D'Albis Giuseppe , D'Albis Vincenzo , Palma Micol , Plantamura Marianna , Nizar Al krenawi 
Published: August 2022

SummaryHyaluronic acid (HA) has been widely used in medicine and is currently of particular interest to maxillofacial surgeons. Several applications have been introduced, including those in which HA is used as a scaffold for bone regeneration, either alone or in combination with other grafting materials, to enhance bone growth. This review aims to analyze the available literature on the use of HA for maxillofacial bone regenerative procedures including socket preservation, sinus augmentation, and ridge augmentation. Medline and PubMed databases were searched for relevant reports published between January 2000 and April 2021. Nine publications describing the use of HA to augment bone volume were identified. Although further studies are needed, these findings are encouraging as they suggest that HA could be used effectively used, in combination with graft materials, in maxillofacial bone regenerative procedures. HA facilitates manipulation of bone grafts, improves handling characteristics and promotes osteoblast activity that stimulates bone regeneration and repair.
Prevalence and prognostic implications of reduced left ventricular ejection fraction among patients with STEMI in India
Authors: Hendrickson Michael J. , Arora Sameer , Vaduganathan Muthiah , Fonarow Gregg C. , MP Girish , Bansal Ankit , Batra Vishal , Kunal Shekhar , Bhatt Deepak L. , Gupta Mohit , Qamar Arman 
Published: August 2022

AbstractAimsTo describe clinical characteristics and outcomes for those with STEMI and reduced left ventricular ejection fraction (LVEF) in low‐income and middle‐income countries (LMICs).Methods and resultsAdults presenting with STEMI to two government‐owned tertiary care centres in Delhi, India were prospectively enrolled in the North India ST‐elevation myocardial infarction (NORIN‐STEMI) registry. LVEF was evaluated at presentation and clinical characteristics were compared across LVEF categories. Overall, 3597 patients were included, of whom 468 (13%) had LVEF >50%, 1482 (41%) had mildly reduced LVEF (40–49%), 1357 (38%) had moderately reduced LVEF (30–39%), and 290 (8%) had severely reduced LVEF (<30%). Presentation delay >24 h, prior MI, and hyperlipidaemia were associated with decreasing LVEF category. Although most patients with reduced LVEF were discharged on appropriate guideline‐directed therapies, adherence at 1 year was low (ACE inhibitor/ARB 91% to 41%, beta blocker 98% to 78%, aldosterone receptor antagonist 69% to 6%). After multivariable adjustment, a Cox regression model showed moderately reduced LVEF (HR 1.77, 95% CI 1.20, 2.60) and severely reduced LVEF (HR 3.63, 95% CI 2.41, 5.48) were associated with increased risk of all‐cause mortality compared with LVEF ≥50%.ConclusionsOn presentation for STEMI, almost 90% of NORIN‐STEMI participants had at least mildly reduced LVEF and almost half had LVEF <40%. Patients with LVEF <40% had significantly higher risk of mortality at 1 year, and adherence to guideline‐directed therapies at 1 year was poor. Systematic initiatives to improve access to timely revascularization and guideline‐directed therapies are essential in advancing STEMI care in LMICs.
Healthcare costs of a telemonitoring programme for heart failure: indirect deterministic data linkage analysis
Authors: Pathak Atul , Levy Pierre , Roubille François , Chatellier Gilles , Mercier Grégoire , Alami Sarah , Lancman Guila , Pasche Hélène , Laurelli Corinne , Delval Cécile , Ramirez‐Gil Juan Fernando , Galinier Michel 
Published: August 2022

AbstractAimsWe aim to evaluate the costs associated with healthcare resource consumption for chronic heart failure (HF) management in patients allocated to telemonitoring versus standard of care (SC).Methods and resultsOSICAT‐ECO involved 745 patients from the OSICAT trial (NCT02068118) who were successfully linked to the French national healthcare database through an indirect deterministic data linkage approach. OSICAT compared a telemonitoring programme with SC follow‐up in adults hospitalized for acute HF ≤ 12 months. Healthcare resource costs included those related to hospital and ambulatory expenditure for HF and were restricted to direct costs determined from the French health data system over 18 months of follow‐up. Most of the total costs (69.4%) were due to hospitalization for HF decompensation, followed by ambulatory nursing fees (11.8%). During 18‐month follow‐up, total costs were 2% lower in the telemonitoring versus the SC group, due primarily to a 21% reduction in nurse fees. Among patients with NYHA class III/IV, a 15% reduction in total costs (€3131 decrease) was observed over 18‐month follow‐up in the telemonitoring versus the SC group, with the highest difference in hospital expenditure during the first 6 months, followed by a shift in costs from hospital to ambulatory at 12 months.ConclusionsHF hospitalization and ambulatory nursing fees represented most of the costs related to HF. No benefit was observed for telemonitoring versus SC with regard to cost reductions over 18 months. Patients with severe HF showed a non‐significant 15% reduction in costs, largely related to hospitalization for HF decompensation, nurse fees, and medical transport.
Distribution network reconfiguration for minimizing impact of wind power curtailment on the network losses: A two‐stage stochastic optimization algorithm
Authors: Azad‐Farsani Ehsan , Zeinoddini‐Meymand Hamed , Jafari Hamed 
Published: August 2022

AbstractNowadays, with the increasing penetration rate of wind turbine generators (WTGs) in power distribution networks, possible network‐related problems should be considered. Distribution network reconfiguration (DNR) can reduce the effect of wind power curtailment by transferring loads to the other feeders and compensating for wind forecast error. In this paper, a two‐stage policy is presented to deal with the network configuration and wind power uncertainty. In the first stage, the scheduled output of WTGs is determined, and the wind power uncertainty is modeled using a scenario‐based approach. In the second stage, a modified firework (MFW) algorithm is considered for the network reconfiguration to minimize the network losses. In the MFW algorithm, a new local search method is implemented to enhance the performance of the FW algorithm by increasing the direction of the explosion. The impact of wind power curtailment in the network operation is minimized using the proposed policy that changes the network configuration. The proposed methodology is tested on a 70‐bus IEEE distribution test system. The simulation results demonstrate the efficiency of the proposed algorithm in solving the DNR problem in the presence of WTGs, considering the uncertainty of wind speed.
Targeting the source of fine sediment and associated geochemical elements by using novel fingerprinting methods in proglacial tropical highlands (Cordillera Blanca, Perú)
Authors: Navas Ana , Lizaga Iván , Santillán Nelson , Gaspar Leticia , Latorre Borja , Dercon Gerd 
Published: August 2022

AbstractIn the Cordillera Blanca, the glaciers delineated by some of the highest peaks in the tropical Peruvian Andes have experienced a fast retreat over the last few decades. At the foot of Artesonraju Peak, the glacier‐fed river conveys fine sediments to Parón Lake, which is enclosed in the proglacial area created by the retreating glaciers of the unique confluence of the Huandoy, Chacraraju, Nevado Caraz and Artesonraju peaks (above 6000 m.a.s.l.). This research addresses the sediment contributions of main glacial landforms to sediment mixtures delivered to the river and lake system (1.6 km2) by applying novel conservativeness and consistency methods for tracer selection and a frequentist unmixing model. A total of 27 surface soil and sediment sources (0–3 cm) were sampled from moraines, alluvial fans and recent colluvium, and 10 composite sediment mixtures, including suspended sediments, were collected to identify the sediment provenance. The unmixing results reveal large variability of the contributing sources related to their predominance and the locations of the mixtures. The largest contributions were from moraines, in percentages varying from 28% to as much as 77%. Alluvial fans contributed between 23% and 47% of the sediments, and colluvium material contributed the least, between 6% and 28%. At the time of sampling, ice melt discharge incising moraines and alluvial fans and eroding riverbanks were the main mechanisms of fine sediment delivery. Runoff over the bare surfaces of recently exposed moraines and the lack of vegetation cover in very active landforms also control sediment mobilization. Overall, highly dynamic changes in this paraglacial environment involve active sediment fluxes accompanying snow and ice melt. Our results indicate that in high tropical mountains, shrinking glaciers expose new surfaces of glacial materials, which deliver fine sediments. Identifying the contributing sources is of interest for water and sediment evaluation and management programs.
Leptomeningeal carcinomatosis in small cell carcinoma of the prostate
Authors: Fukuta Kyotaro , Shiozaki Keito , Nakanishi Ryoichi , Izaki Hirofumi , Kudo Eiji , Fukawa Tomoya , Yamaguchi Kunihisa , Yamamoto Yasuyo , Takahashi Masayuki , Kanayama Hiro‐omi 
Published: August 2022

IntroductionLeptomeningeal carcinomatosis in small cell carcinoma of the prostate is rare.Case presentationA 69‐year‐old man visited our hospital due to dysuria and edema. Bilateral hydronephrosis and lymph node metastases due to a pelvic tumor were observed. Although the prostate‐specific antigen level was normal, the tumor was suspected to originate from the prostate. He underwent percutaneous nephrostomy and prostate biopsy. Histopathology revealed small cell carcinoma accompanied by increased pro‐gastrin‐releasing peptide and neuron‐specific enolase levels. After receiving systemic chemotherapy with carboplatin and etoposide and radiation therapy for prostate, these lesions gradually decreased in size, and tumor markers normalized. Ten months after the initial diagnosis, he developed consciousness disorder and seizure. Magnetic resonance imaging revealed leptomeningeal carcinomatosis without any other recurrences and elevated tumor markers. He died 4 weeks after these symptoms appeared.ConclusionCareful monitoring of the central nervous system should be considered in small cell carcinoma of the prostate patients.
Assessing ring‐shaped subsurface irrigation system through a conventional subsurface emitter
Authors: Norouzian Zahra , Ahmadi Hojjat 
Published: August 2022

AbstractA ring‐shaped emitter is a cheap subsurface irrigation system recommended for small‐scale farms. This study compared the performance of a ring‐shaped emitter with conventional subsurface drip irrigation (SDI) under the same conditions. HYDRUS, which is a useful software that can thoroughly and adequately simulate water movement in the soil under different irrigation systems, was used to study the performance of the aforementioned irrigation systems. We used field data to calibrate and verify the numerical model. The water distribution around the irrigation systems studied was modelled as 2D in‐plane strain for ordinary SDI (vertical plane XZ) and axisymmetric geometrical states for the ring‐shaped emitter. This study mainly evaluated ring‐shaped emitters and compared them with SDI to verify whether these emitters could be a recommended alternative irrigation method in small‐scale farms. The simulation results showed that water availability in the exact coordinates in both emitters was approximately identical, except near the waterfront. Water movement in the ring‐shaped emitter was faster than that in SDI, and therefore, the waterfront in the ring‐shaped emitter was further away at all times. Finally, the water entrance to the second layer under this emitter met with less resistance.
Pre‐glycation impairs gelation of high concentration collagen solutions
Authors: Diamantides Nicole , Slyker Leigh , Martin Sara , Rodriguez Marcos R. , Bonassar Lawrence J. 
Published: August 2022

AbstractThere remains a need for stiffer collagen hydrogels for tissue engineering and disease modeling applications. Pre‐glycation, or glycation of collagen in solution prior to gelation, has been shown to increase the mechanics of collagen hydrogels while maintaining high viability of encapsulated cells. The stiffness of glycated collagen gels can be increased by increasing the collagen concentration, sugar concentration, and glycation time. However, previous studies on pre‐glycation of collagen have used low collagen concentrations and/or low sugar concentrations and have not investigated the effect of glycation time. Therefore, the objective of this study was to determine the effects of pre‐glycation with high sugar concentrations (up to 500 mM) and extended glycation times (up to 21 days) on high concentration collagen (8 mg/ml). The addition of sugar to the collagen and the formation of advanced glycation end products (AGEs) were quantified. The ability to gel successfully and rheological properties were determined and correlated with biochemical characterizations. Successful collagen gelation and rheological properties of pre‐glycated collagen were found to be strongly dependent on the ratio of added sugars to added AGEs with high ratios impairing gelation and low ratios resulting in optimal storage moduli. There is likely a competing effect during pre‐glycation of the formation of AGEs resulting in crosslinking of collagen and the formation of Amadori intermediates acting to increase collagen solubility. Overall, this study shows that collagen glycation can be optimized by increasing the formation of AGEs while maintaining a low ratio of added sugar to added AGEs.
A novel algorithm for lung adenocarcinoma based on N6 methyladenosine‐related immune long noncoding RNAs as a reliable biomarker for predicting survival outcomes and selecting sensitive anti‐tumor therapies
Authors: Yan Qiuwen , Hu Bingchuan , Chen Hang , Zhu Linwen , Lyu Yao , Qian Dingding , Shao Guofeng 
Published: August 2022

AbstractBackgroundLung cancer is a highly heterogeneous malignant tumor with high incidence and mortality. Recently, increasing evidence has demonstrated that N6‐methyladenosine (m6A) methylation and the tumor microenvironment (TME) play important roles in the occurrence and development of lung adenocarcinoma (LUAD).MethodsIn this study, we constructed a novel and reliable algorithm based on m6A‐related immune lncRNAs (mrilncRNAs), consisting of molecular subtypes and a prognostic signature.ResultsAccording to the analyses of molecular subtypes, patients in cluster 1 were in a more advanced stage, showed poor prognosis, were sensitive to immunotherapy (anti‐programmed cell death 1 Ligand 1 (PD‐L1) and anti‐lymphocyte activating 3 (LAG‐3)), and had a highest tumor mutational burden (TMB), while anti‐cytotoxic T‐lymphocyte‐associated protein 4 (CTLA‐4) therapy seemed to be a good choice for patients in cluster 3. Subsequently, the results of the risk assessment model indicated that the low‐risk patients exhibited a survival advantage, had an earlier stage, and showed a higher response to common anti‐cancer drugs, including chemotherapy (Docetaxel, Paclitaxel), molecular targeted therapy (Erlotinib), and immunotherapy (anti‐CTLA‐4 therapy), while Gefitinib could be a good choice for patients with high‐risk scores.ConclusionIn conclusion, the constructed algorithm exhibits promising practical prospects, and allows the selection of suitable and sensitive anti‐cancer drugs, which could provide theoretical support to predict the survival outcomes of patients with LUAD.
LAMTOR3 is a prognostic biomarker in kidney renal clear cell carcinoma
Authors: Gong Yun , Lv Yue , Xu Fanghua , Xiu Youcheng , Lu Yinhui , Liu Zan , Deng Leihong 
Published: August 2022

AbstractObjectiveThe objective of the study was to investigate the expression of LAMTOR3 in kidney renal clear cell carcinoma (KIRC) and its clinical significance.MethodsThe expression of LAMTOR3 in KIRC and its relationship with clinical features were analyzed using the UALCAN online database. The results were verified using KIRC gene chip data and clinical specimens. The prognosis of KIRC patients was analyzed with the GEPIA2 database. GO, KEGG, and GSEA analyses were conducted to analyze the possible molecular mechanism of LAMTOR3 in KIRC. Immunohistochemical (IHC) and hematoxylin and eosin (H&E) staining were used for histopathological detection.ResultsUALCAN database analysis showed that LAMTOR3 expression in KIRC was significantly lower than in normal kidney tissues and correlated with the clinical stage, pathological grade, and tumor genotype (p < .05). GSE53757 dataset analysis consistently showed that the expression of LAMTOR3 in KIRC was significantly lower than in normal kidney tissues (p < .01). GEPIA2 database analysis indicated that patients with low LAMTOR3 expression had poor overall and disease‐free survival rates. GSEA analysis suggested that LAMTOR3 positively regulated the citrate cycle and drug metabolism cytochrome P450 and negatively regulated folate biosynthesis and olfactory transduction. The expression of LAMTOR3 in KIRC was also significantly correlated with immune cell infiltration. Finally, IHC showed that LAMTOR3 expression in the KIRC tissues was lower than in the adjacent normal tissues.ConclusionLAMTOR3 expression is significantly lower in KIRC. LAMTOR3 may be a potential marker for KIRC diagnosis and therapy.
Broad application prospects of bone turnover markers in pediatrics
Authors: Zhang Yiduo , Huang Xiaocui , Li Chao , Zhang Jing , Yu Xingnan , Li Ye , Zhou Wenjie , Yu Fan 
Published: August 2022

AbstractBackgroundBone turnover markers (BTMs) have been studied for application in clinical medicine. However, BTMs in children are challenging, and few studies explore these BTMs in children. The application of BTMs is complicated mainly due to pre‐analytical factors, variable reference intervals of age‐ and sex‐related BTMs for adolescents and children in different regions and laboratories. Therefore, laboratory testing of BTMs is critical for understanding pediatric bone development and metabolism, which provides additional information about bone development and diseases.MethodsLiterature search was conducted using the MeSH term “child” combined with the terms that bone turnover markers such as “osteocalcin,” “Procollagen type I N‐terminal propeptide,” “procollagen type I C‐terminal propeptide,” “osteocalcin,” “N‐terminal cross‐linked telopeptide,” and “C‐terminal cross‐linked telopeptide,” Several databases including Web of Science, Google Scholar, and PubMed were searched to obtain the relevant studies.ResultsBTMs represent the combined effects of skeletal development, growth, and remodeling in children, which can be used in clinical pediatrics to assist in the diagnosis and prognosis of bone metabolic disorders.ConclusionBTMs are clearly helpful for diagnosis and monitoring of bone growth and development as well as bone metabolic disorders.