bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is the popliteal tendon an adequate anchor for lateral meniscus repairs? A cadaveric study
Authors: Séguineau Arthur , Pujol Nicolas , Noailles Thibaut 
Published: March 2022

Objectives: Arthroscopic lateral meniscus repairs are performed in the Cabot test position. Anchor fixation to the popliteal tendon is often the primary anchor. Our hypothesis was that the mobility of the popliteal tendon in flexion-extension leads to significant stress or even early loosening of sutures. Methods: Twenty-two fresh adult human cadaveric knees were used. A vertical stitch was made arthroscopically with "all-internal" implants in the popliteal tendon through the lateral meniscus. The knee was taken from the Cabot test position and placed in full extension at the end of the procedure. The condition of the suture was then assessed under arthroscopy. Sutures were considered to have failed if they had sufficient slack to pass the arthroscope through the hiatus, if the knot had passed through the meniscus, or if the thread was broken. Results: 59.9% (n = 9) of the stitches were tight and 40.1% were loose (n = 9). 18.2% (n = 4) were relaxed, for 18.2% (n = 4) the knot had passed through the meniscus, and 4.5% (n = 1) had a break in the suture. Conclusion: Sutures placed between the lateral meniscus and the popliteal tendon failed after a single cycle of knee flexion and extension in 40% of the cases. A single stitch in this manner is not an adequate means of repairing lateral meniscal injuries.
The combination of a tibial slope correction osteotomy with ACL revision at a mean follow-up of 10 years provides good clinical results without revision surgery
Authors: Rozinthe Anouk , Demey Guillaume , Van Rooij Floris , Saffarini Mo , Dejour David 
Published: March 2022

Objectives: The objective is to discover the long-term outcome of ACL reconstruction associated with tibial slope correction osteotomy for a second ACL tear. This study is preceded by an initial publication with a 4-year follow-up. The hypothesis was stable functional scores and the absence of osteoarthritis progression. Methods: The initial consecutive retrospective series included 9 patients who had a revision surgery for ACL reconstruction associated with single-stage OTDF. They were reviewed by an independent observer for a second follow-up with a minimum retrospect of 7 years. The analysis was based on IKDC-SKF and Lysholm scores, and radiographic analysis. Results: Of the original 9 patients, 7 were seen again, 1 was only interviewed by telephone, and 1 was lost to follow-up. At the last follow-up of 9.9 ± 3.0 years, all 8 patients had stable or improved functional scores compared to the previous follow-up. The mean Lysholm score increased from 73.8 ± 5.8 (65-82) to 84.5 ± 11.9 (59-95) and the mean IKDC-SKF score increased from 71.6 ± 6.2 (62-79) to 82.9 ± 12.1 (61-98). Of the six patients who already had proof of osteoarthritis at the previous follow-up, the grade of osteoarthritis increased for two (with a history of meniscectomy, from grade 2 to grade 3 and from grade 1 to grade 3) but remained stable for three patients and could not be assessed for one. Conclusion: At 7-15 years (mean: 9.9 years), patients’ functional scores were stable or improving compared to the previous 2-8 year follow-up period, with no new tear or revision surgery. This validates tibial slope correction after recurrent ACL tears with an increase in the tibial slope
Meniscal tears and entrapment in intercondylar eminence fractures in children: a systematic review of the literature
Authors: Severyns Mathieu , Arthur Renault , Plawecki Stéphane 
Published: March 2022

Objectives: Although they may be related to ACL tears in adults, there are few publications on meniscal injuries after intercondylar eminence fractures in children. Methods: The main objective of this systematic review of the literature was to measure the incidence of meniscal injuries as well as concomitant tissue involvement in tibial eminence fractures (TEF) in children. The secondary objectives were to determine a diagnostic and therapeutic strategy to anticipate difficulties in reduction and to treat associated meniscal tears. The PubMed and Scopus database subject search included MeSH indexing terms: "intercondylar tibial fracture*" OR "tibial spine fracture*" OR “tibial spinal fracture” OR "tibial eminence fracture*" OR "intercondylar eminence fracture*". Only series of pediatric tibial eminence fractures with the presence of data on possible meniscal damage were selected. Case reports, mixed adult and child series and duplicates were excluded. Results: 789 references were chosen with 91 eligible articles. Only 26 articles reported on the rates of meniscal tears and entrapment after these fractures. Of a total of 997 cases, 227 (22.8%) had a concomitant injury (osteochondral, ACL, PCL and/or meniscal), including 180 cases of meniscal tears (18.1%). Meniscal or intermeniscal ligament entrapment was present in 20.1% (n = 201) of the patients. 2 groups of diagnostic analyses of these injuries were compared: during the arthroscopy, or previously on MRI imaging. Meniscal tears were present in 20.9% of the patients in the arthroscopy group, compared with 26.1% in the group with a prior MRI (p = .06), and the proportion of meniscal tears was 18.6% in the arthroscopy group alone compared to 17.1% in the MRI group (p = .55). However, statistically, the proportion of entrapment noted was significantly higher in the arthroscopy group (17.8% vs 6.2%; p <.0001). Conclusion: This review of the literature reports a high incidence of meniscal tears and tissue entrapment in children. Although it would appear that MRI tends to improve the assessment of associated injuries, the rate of meniscal or intermeniscal ligament entrapment is low compared to the rate reported by arthroscopic diagnosis.
Validity and reproducibility of the PPLP score in the follow-up of anterior cruciate ligament reconstruction in athletes
Authors: Laboute Eric , Garrigou Pierre , Ucay Olivier , Verhaeghe Emmanuel 
Published: March 2022

Objectives: In a context where psychological scores appear to have an increasing influence on the resumption of sports, it would also appear to be essential to evaluate the relevance of global scores with multiple criteria. The PPLP score (Psychological Physical Ligament Predictive score) enables an analysis of subjective, clinical and isokinetic muscle assessment items. Methods: We prospectively followed anterior cruciate ligament (ACL) reconstructions in several series for which we analyzed the validity of the score (763 patients), the reproducibility, the sensitivity to change, the relevance in the follow-up of ligamentoplasty (3,296 ligamentoplasties followed postoperatively) as well as the predictive nature in the resumption of sports (258 patients). The PPLP score has two parts: one (PPLP1) based on 100 points for postoperative follow-up and the other also based on 100 points (PPLP2) which is added to the first score for follow-up at a certain period of time after surgery to determine a new score of 200 points. We also tried to identify correlations with other scores in the literature (OAK, Lysholm, Tegner, KOOS, Arpeggio, IKDC Subjective and the Psychovitality Test). Results: We demonstrated the distinction of the items (r coefficient of 0.20) for construct validity. Intra-examiner and extra-examiner reproducibility was excellent, ranging from 0.92 to 1. The PPLP score changed in a statistically significant way during hospitalization and according to the operating lead time with phases of higher variation. Complicated clinical changes manifested in a low score, in particular neuro-algodystrophies (1.9%) with a mean PPLP1 of 80.33 while changes without complications (80.8%) had a mean score of 94.28 with a significant difference (p < 0.0001). The PPLP2 score correlated significantly with the resumption of competition (p = 0.012). A high score was associated with faster recovery. The optimal cut-off score was 176 (sensitivity 79.7% and specificity 49.3%). The results of the PPLP score were correlated with the other scores. Conclusion: The PPLP score was validated in terms of construction, reproducibility, and sensitivity in the follow-up of ACL ligamentoplasties. It provides indications on the quality of change and the possibilities of resuming sports. It is the only score that incorporates isokinetic assessments.
Plantar Plating for Medial Naviculocuneiform Arthrodesis in Progressive Collapsing Foot Deformity
Authors: Wininger Austin E. , Klavas Derek M. , Gardner Stephanie S. , Ahuero Jason S. , Harris Joshua D. , Varner Kevin E. 
Published: March 2022

Background: Medial column procedures are commonly used to treat progressive collapsing foot deformity (PCFD) reconstruction. The aim of this research is to present the clinical results of plantar plating for medial naviculocuneiform (NC) arthrodesis when NC joint pathology contributes to medial arch collapse. The authors hypothesized that lag screws with a plantar neutralization plate would result in a satisfactory NC joint fusion rate. Methods: A single-surgeon, retrospective case series was performed on patients with flexible PCFD who underwent NC arthrodesis using lag screws and a contoured neutralization plate applied plantarly across the medial NC joint as part of PCFD reconstruction. Thirteen patients (11 females, 2 males; mean age 53.1 [34-62] years) between 2016 and 2019 were identified for inclusion. Mean follow-up was 25.2 ± 12.7 months. Preoperative and postoperative anteroposterior talo–first metatarsal angle, lateral talo–first metatarsal angle, talonavicular coverage angle, and calcaneal pitch were measured. Union was evaluated radiologically. AOFAS midfoot scores were recorded at final follow-up. Results: All parameters demonstrated a significant improvement. Fusion was confirmed in 11 of 13 patients (85%) at a mean 5.7 ± 2.1 months. One patient required a revision of their NC fusion because of symptomatic nonunion. There were no cases of symptomatic plantar hardware. Conclusion: The results of this small cohort series suggest that lag screw with plantar plate NC arthrodesis yielded generally improved short-term radiographic and clinical outcomes in PCFD patients with medial arch collapse through the NC joint. Level of Evidence: Level IV, retrospective case series.
Therapeutic Effect of Multi-Channel Transcranial Direct Current Stimulation (M-tDCS) on Recovery of Cognitive Domains, Motor Functions of Paretic Hand and Gait in Subacute Stroke Survivors-A Randomized Controlled Trial Protocol
Authors: Divya Midha , Narkeesh Arumugam 
Published: March 2022

Background: There has been rapid drift of rehabilitation professionals toward the clinical use of technology aided electrical interventions. Brain is a cortical hub of functionally related neural connections. Motor learning entails strong interaction with the cognitive domains. So better outcomes may be expected by optimally targeting functionally correlated areas simultaneously through tDCS. Aim: To determine the therapeutic effect of Multi Channel tDCS in combination with Functional electrical stimulation, SaeboFlex and conventional rehabilitation on recovery of Cognitive Domains, Motor Functions of Paretic Hand, and Gait in individuals with subacute Stroke. Methods: This is prospective, randomized, double blind controlled clinical trial. Subacute Stroke Survivors with the age Group (40-75 years) will constitute the Population of the study. Participants will be randomly allocated to experimental or control group. Participants of Experimental group will receive Multi channel tDCS, Functional electrical stimulation, Saebo Flex Training and conventional rehabilitation. Participants of the group B will receive FES, training with SaeboFlex, conventional physiotherapy intervention similar to as given to the participants of group A and sham multi channel tDCS. Outcome Measures: The primary outcome measures of the study will be Fugl Meyer assessment, Electroencephalogram and secondary outcome measures of the study will be Grip strength, Pinch strength, Nine hole peg test( NHPT), Wisconsin gait scale, Montreal cognitive assessment, Electroencephaloraphy to observe the cortical changes and tDCS adverse effect questionnaire and stroke specific quality of Life scale. Statistical Analysis: The primary analysis of the study will be done at the end of 4 weeks. Statistical analysis of data will be done using SPSS Version 22 with the help of a statistician. Conclusion: An insight into the therapeutic interventions augmenting, cognitive and motor domains simultaneously may yield better outcomes in the field of stroke rehabilitation thereby improving quality of life of stroke survivors
Macrophage NOX2 NADPH oxidase maintains alveolar homeostasis in mice
Authors: Bhattacharya Sourav , Idol Rachel A. , Yang Wei , Rojas Márquez Jorge David , Li Yanan , Huang Guangming , Beatty Wandy L. , Atkinson Jeffrey J. , Brumell John H. , Bagaitkar Juhi , Magee Jeffrey A. , Dinauer Mary C. 
Published: March 2022

Abstract
The leukocyte NADPH oxidase 2 (NOX2) plays a key role in pathogen killing and immunoregulation. Genetic defects in NOX2 result in chronic granulomatous disease (CGD), associated with microbial infections and inflammatory disorders, often involving the lung. Alveolar macrophages (AMs) are the predominant immune cell in the airways at steady state, and limiting their activation is important, given the constant exposure to inhaled materials, yet the importance of NOX2 in this process is not well understood. In this study, we showed a previously undescribed role for NOX2 in maintaining lung homeostasis by suppressing AM activation, in CGD mice or mice with selective loss of NOX2 preferentially in macrophages. AMs lacking NOX2 had increased cytokine responses to Toll-like receptor-2 (TLR2) and TLR4 stimulation ex vivo. Moreover, between 4 and 12 week of age, mice with global NOX2 deletion developed an activated CD11bhigh subset of AMs with epigenetic and transcriptional profiles reflecting immune activation compared with WT AMs. The presence of CD11bhigh AMs in CGD mice correlated with an increased number of alveolar neutrophils and proinflammatory cytokines at steady state and increased lung inflammation after insults. Moreover, deletion of NOX2 preferentially in macrophages was sufficient for mice to develop an activated CD11bhigh AM subset and accompanying proinflammatory sequelae. In addition, we showed that the altered resident macrophage transcriptional profile in the absence of NOX2 is tissue specific, as those changes were not seen in resident peritoneal macrophages. Thus, these data demonstrate that the absence of NOX2 in alveolar macrophages leads to their proinflammatory remodeling and dysregulates alveolar homeostasis.
Noninvasive follow-up strategy after pulmonary endarterectomy for chronic thromboembolic pulmonary hypertension
Authors: Ruigrok Dieuwertje , Handoko M. Louis , Meijboom Lilian J. , Nossent Esther J. , Boonstra Anco , Braams Natalia J. , van Wezenbeek Jessie , Tepaske Robert , Tuinman Pieter Roel , Heunks Leo M.A. , Vonk Noordegraaf Anton , de Man Frances S. , Symersky Petr , Bogaard Harm-Jan 
Published: March 2022

BackgroundThe success of pulmonary endarterectomy (PEA) for chronic thromboembolic pulmonary hypertension (CTEPH) is usually evaluated by performing a right heart catheterisation (RHC). Here, we investigate whether residual pulmonary hypertension (PH) can be sufficiently excluded without the need for a RHC, by making use of early post-operative haemodynamics, or N-terminal pro-brain natriuretic peptide (NT-proBNP), cardiopulmonary exercise testing (CPET) and transthoracic echocardiography (TTE) 6 months after PEA.MethodsIn an observational analysis, residual PH after PEA measured by RHC was related to haemodynamic data from the post-operative intensive care unit time and data from a 6-month follow-up assessment including NT-proBNP, TTE and CPET. After dichotomisation and univariate analysis, sensitivity, specificity, positive predictive value, negative predictive value (NPV) and likelihood ratios were calculated.ResultsThirty-six out of 92 included patients had residual PH 6 months after PEA (39%). Correlation between early post-operative and 6-month follow-up mean pulmonary artery pressure was moderate (Spearman rho 0.465, p<0.001). Early haemodynamics did not predict late success. NT-proBNP >300 ng·L−1had insufficient NPV (0.71) to exclude residual PH. Probability for PH on TTE had a moderate NPV (0.74) for residual PH. Peak oxygen consumption (V′O2) <80% predicted had the highest sensitivity (0.85) and NPV (0.84) for residual PH.ConclusionsCPET 6 months after PEA, and to a lesser extent TTE, can be used to exclude residual CTEPH, thereby safely reducing the number of patients needing to undergo re-RHC after PEA.
Facile Green Synthesis of Titanium Dioxide Nanoparticles by Upcycling Mangosteen (Garcinia mangostana) Pericarp Extract
Authors: Ahn Eun-Young , Shin Sang-Woo , Kim Kyeongsoon , Park Youmie 
Published: March 2022

AbstractIn the present report, green synthesis of titanium dioxide nanoparticles (TiO2 NPs) was performed by upcycling mangosteen (Garcinia mangostana) pericarp extract (methanol and ethyl acetate extracts). Field emission scanning electron microscopy images revealed an aggregated structure with a highly porous network of TiO2 NPs. TiO2 NPs synthesized with ethyl acetate extract (EtOAc-TiO2 NPs) exhibited more monodispersity and possessed smoother surfaces than the control TiO2 NPs (Con-TiO2 NPs) and TiO2 NPs synthesized with methanol extract (MeOH-TiO2 NPs). High-resolution X-ray diffraction patterns clearly confirmed that TiO2 NPs had a crystalline nature. A mixture of anatase and rutile was observed in Con-TiO2 NPs and MeOH-TiO2 NPs, while EtOAc-TiO2 NPs had only anatase with the smallest size (12.50 ± 1.81 nm). Ethyl acetate extract contained the highest amount of α-mangostin; thus, the surface of TiO2 NPs was functionalized with ethyl acetate extract. The functionalized TiO2 NPs synthesized with ethyl acetate extract (EtOAc-TiO2-αm) showed the highest 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) radical scavenging activity. In vitro cell viability on mouse fibroblast cells (NIH3T3) indicated that the newly synthesized TiO2 NPs did not show any significant cytotoxicity. Therefore, the TiO2 NPs in the present report have the potential to be used in cosmetic applications such as sunscreens.
LPMX: a pure rootless composable container system
Authors: Yang Xu , Kasahara Masahiro 
Published: March 2022

Abstract
Background
Delivering tools for genome analysis to users is often difficult given the complex dependencies and conflicts of such tools. Container virtualization systems (such as Singularity) isolate environments, thereby helping developers package tools. However, these systems lack mutual composability, i.e., an easy way to integrate multiple tools in different containers and/or on the host. Another issue is that one may be unable to use a single container system of the same version at all the sites being used, thus discouraging the use of container systems.

Results
We developed LPMX, an open-source pure rootless composable container system that provides composability; i.e., the system allows users to easily integrate tools from different containers or even from the host. LPMX accelerates science by letting researchers compose existing containers and containerize tools/pipelines that are difficult to package/containerize using Conda or Singularity, thereby saving researchers’ precious time. The technique used in LPMX allows LPMX to run purely in userspace without root privileges even during installation, thus ensuring that we can use LPMX at any Linux clusters with major distributions. The lowest overhead for launching containers with LPMX gives us courage to isolate tools as much as possible into small containers, thereby minimizing the chance of conflicts. The support for the layered file system keeps the total size of container images for a single genomic pipeline modest, as opposed to Singularity, which uses mostly a flat single-layer image.

Conclusions
LPMX is pure rootless container engine with mutual composability, thus saving researchers’ time, and accelerating science.
Development of a versatile resource for post-genomic research through consolidating and characterizing 1500 diverse wild and cultivated soybean genomes
Authors: Zhang Hengyou , Jiang He , Hu Zhenbin , Song Qijian , An Yong-qiang Charles 
Published: March 2022

AbstractBackgroundWith advances in next-generation sequencing technologies, an unprecedented amount of soybean accessions has been sequenced by many individual studies and made available as raw sequencing reads for post-genomic research.ResultsTo develop a consolidated and user-friendly genomic resource for post-genomic research, we consolidated the raw resequencing data of 1465 soybean genomes available in the public and 91 highly diverse wild soybean genomes newly sequenced. These altogether provided a collection of 1556 sequenced genomes of 1501 diverse accessions (1.5 K). The collection comprises of wild, landraces and elite cultivars of soybean that were grown in East Asia or major soybean cultivating areas around the world. Our extensive sequence analysis discovered 32 million single nucleotide polymorphisms (32mSNPs) and revealed a SNP density of 30 SNPs/kb and 12 non-synonymous SNPs/gene reflecting a high structural and functional genomic diversity of the new collection. Each SNP was annotated with 30 categories of structural and/or functional information. We further identified paired accessions between the 1.5 K and 20,087 (20 K) accessions in US collection as genomic “equivalent” accessions sharing the highest genomic identity for minimizing the barriers in soybean germplasm exchange between countries. We also exemplified the utility of 32mSNPs in enhancing post-genomics research through in-silico genotyping, high-resolution GWAS, discovering and/or characterizing genes and alleles/mutations, identifying germplasms containing beneficial alleles that are potentially experiencing artificial selection.ConclusionThe comprehensive analysis of publicly available large-scale genome sequencing data of diverse cultivated accessions and the newly in-house sequenced wild accessions greatly increased the soybean genome-wide variation resolution. This could facilitate a variety of genetic and molecular-level analyses in soybean. The 32mSNPs and 1.5 K accessions with their comprehensive annotation have been made available at the SoyBase and Ag Data Commons. The dataset could further serve as a versatile and expandable core resource for exploring the exponentially increasing genome sequencing data for a variety of post-genomic research.