bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro and in ovo impact of the ionic dissolution products of boron-doped bioactive silicate glasses on cell viability, osteogenesis and angiogenesis
Authors: Decker Simon , Arango-Ospina Marcela , Rehder Felix , Moghaddam Arash , Simon Rolf , Merle Christian , Renkawitz Tobias , Boccaccini Aldo R. , Westhauser Fabian 
Published: May 2022

AbstractDue to the pivotal role of angiogenesis in bone regeneration, the angiogenic properties of biomaterials are of high importance since they directly correlate with the biomaterials’ osteogenic potential via ‘angiogenic-osteogenic coupling’ mechanisms. The impact of bioactive glasses (BGs) on vascularization can be tailored by incorporation of biologically active ions such as boron (B). Based on the ICIE16-BG composition (in mol%: 49.5 SiO2, 36.3 CaO, 6.6 Na2O, 1.1 P2O5, 6.6 K2O), three B-doped BGs have been developed (compositions in mol%: 46.5/45.5/41.5 SiO2, 36.3 CaO, 6.6 Na2O, 1.1 P2O5, 6.6 K2O, 3/4/8 B2O3). The influence of B-doping on the viability, cellular osteogenic differentiation and expression of osteogenic and angiogenic marker genes of bone marrow-derived mesenchymal stromal cells (BMSCs) was analyzed by cultivating BMSCs in presence of the BGs’ ionic dissolution products (IDPs). Furthermore, the influence of the IDPs on angiogenesis was evaluated in ovo using a chorioallantoic membrane (CAM) assay. The influence of B-doped BGs on BMSC viability was dose-dependent, with higher B concentrations showing limited negative effects. B-doping led to a slight stimulation of osteogenesis and angiogenesis in vitro. In contrast to that, B-doping significantly enhanced vascularization in ovo, especially in higher concentrations. Differences between the results of the in vitro and in ovo part of this study might be explained via the different importance of vascularization in both settings. The implementation of new experimental models that cover the ‘angiogenic-osteogenic coupling’ mechanisms is highly relevant, for instance via extending the application of the CAM assay from solely angiogenic to angiogenic and osteogenic purposes.
Thigh muscle co-contraction patterns in individuals with anterior cruciate ligament reconstruction, athletes and controls during a novel double-hop test
Authors: Arumugam Ashokan , Häger Charlotte K. 
Published: May 2022

AbstractEfficient neuromuscular coordination of the thigh muscles is crucial in maintaining dynamic knee stability and thus reducing anterior cruciate ligament (ACL) injury/re-injury risk. This cross-sectional study measured electromyographic (EMG) thigh muscle co-contraction patterns during a novel one-leg double-hop test among individuals with ACL reconstruction (ACLR;n = 34), elite athletes (n = 22) and controls (n = 24). Participants performed a forward hop followed by a 45° unanticipated diagonal hop either in a medial (UMDH) or lateral direction (ULDH). Medial and lateral quadriceps and hamstrings EMG were recorded for one leg (injured/non-dominant). Quadriceps-to-Hamstring (Q:H) ratio, lateral and medial Q:H co-contraction indices (CCIs), and medial-to-lateral Q:H co-contraction ratio (CCR; a ratio of CCIs) were calculated for three phases (100 ms prior to landing, initial contact [IC] and deceleration phases) of landing. We found greater activity of the quadriceps than the hamstrings during the IC and deceleration phases of UMDH/ULDH across groups. However, higher co-contraction of medial rather than lateral thigh muscles during the deceleration phase of landing was found; if such co-contraction patterns cause knee adduction, a putative mechanism to decrease ACL injury risk, during the deceleration phase of landing across groups warrants further investigation.
Gastric cancer biomarker analysis in patients treated with different adjuvant chemotherapy regimens within SAMIT, a phase III randomized controlled trial
Authors: Oshima Takashi , Tsuburaya Akira , Yoshida Kazuhiro , Yoshikawa Takaki , Miyagi Yohei , Rino Yasushi , Masuda Munetaka , Guan Jia , Tan Patrick , Grabsch Heike I. , Sakamoto Junichi , Tanaka Shiro 
Published: May 2022

AbstractBiomarkers for selecting gastric cancer (GC) patients likely to benefit from sequential paclitaxel treatment followed by fluorinated-pyrimidine-based adjuvant chemotherapy (sequential paclitaxel) were investigated using tissue samples of patients recruited into SAMIT, a phase III randomized controlled trial. Total RNA was extracted from 556 GC resection samples. The expression of 105 genes was quantified using real-time PCR. Genes predicting the benefit of sequential paclitaxel on overall survival, disease-free survival, and cumulative incidence of relapse were identified based on the ranking of p-values associated with the interaction between the biomarker and sequential paclitaxel or monotherapy groups. Low VSNL1 and CD44 expression predicted the benefit of sequential paclitaxel treatment for all three endpoints. Patients with combined low expression of both genes benefitted most from sequential paclitaxel therapy (hazard ratio = 0.48 [95% confidence interval, 0.30–0.78]; p < 0.01; interaction p-value < 0.01). This is the first study to identify VSNL1 and CD44 RNA expression levels as biomarkers for selecting GC patients that are likely to benefit from sequential paclitaxel treatment followed by fluorinated-pyrimidine-based adjuvant chemotherapy. Our findings may facilitate clinical trials on biomarker-oriented postoperative adjuvant chemotherapy for patients with locally advanced GC.
Evaluation of cfDNA as an early detection assay for dense tissue breast cancer
Authors: Barbirou Mouadh , Miller Amanda A. , Gafni Erik , Mezlini Amel , Zidi Asma , Boley Nathan , Tonellato Peter J. 
Published: May 2022

AbstractA cell-free DNA (cfDNA) assay would be a promising approach to early cancer diagnosis, especially for patients with dense tissues. Consistent cfDNA signatures have been observed for many carcinogens. Recently, investigations of cfDNA as a reliable early detection bioassay have presented a powerful opportunity for detecting dense tissue screening complications early. We performed a prospective study to evaluate the potential of characterizing cfDNA as a central element in the early detection of dense tissue breast cancer (BC). Plasma samples were collected from 32 consenting subjects with dense tissue and positive mammograms, 20 with positive biopsies and 12 with negative biopsies. After screening and before biopsy, cfDNA was extracted, and whole-genome next-generation sequencing (NGS) was performed on all samples. Copy number alteration (CNA) and single nucleotide polymorphism (SNP)/insertion/deletion (Indel) analyses were performed to characterize cfDNA. In the positive-positive subjects (cases), a total of 5 CNAs overlapped with 5 previously
reported BC-related oncogenes (KSR2, MAP2K4, MSI2, CANT1 and MSI2). In addition, 1 SNP was detected in KMT2C, a BC oncogene, and 9 others were detected in or near 10 genes (SERAC1, DAGLB, MACF1, NVL, FBXW4, FANK1, KCTD4, CAVIN1; ATP6V0A1 and ZBTB20-AS1) previously associated with non-BC cancers. For the positive–negative subjects (screening), 3 CNAs were detected in BC genes (ACVR2A, CUL3 and PIK3R1), and 5 SNPs were identified in 6 non-BC cancer genes (SNIP1, TBC1D10B, PANK1, PRKCA and RUNX2; SUPT3H). This study presents evidence of the potential of using cfDNA somatic variants as dense tissue BC biomarkers from a noninvasive liquid bioassay for early cancer detection.
Different clinical characteristics and impact of carbapenem-resistance on outcomes between Acinetobacter baumannii and Pseudomonas aeruginosa bacteraemia: a prospective observational study
Authors: Lee Chan Mi , Kim Young-Jun , Jung Sook-In , Kim Seong Eun , Park Wan Beom , Choe Pyoeng Gyun , Kim Eu Suk , Kim Chung-Jong , Choi Hee Jung , Lee Shinwon , Lee Sun Hee , Jung Younghee , Bang Ji Hwan , Cheon Shinhye , Kwak Yee Gyung , Kang Yu Min , Park Kyung-Hwa , Song Kyoung-Ho , Kim Hong Bin ,   The Korea INfectious Diseases (KIND) Study Group, Lee Chan Mi , Kim Young-Jun , Jung Sook-In , Kim Seong Eun , Park Wan Beom , Choe Pyoeng Gyun , Kim Eu Suk , Kim Chung-Jong , Choi Hee Jung , Lee Shinwon , Lee Sun Hee , Jung Younghee , Bang Ji Hwan , Cheon Shinhye , Kwak Yee Gyung , Kang Yu Min , Park Kyung-Hwa , Song Kyoung-Ho , Kim Hong Bin 
Published: May 2022

AbstractThis study aimed to evaluate the differences in clinical characteristics and impact of carbapenem resistance (CR) on outcomes between Acinetobacter baumannii (Ab) and Pseudomonas aeruginosa (Pa) bacteraemia. We prospectively identified all patients with Ab and Pa bacteraemia in 10 hospitals over 1 year. Treatment failure was defined as all-cause 30-day mortality, persistent bacteraemia, or recurrent bacteraemia within 30 days. We included 304 Ab and 241 Pa bacteraemia cases. CR was detected in 216 patients (71%) with Ab bacteraemia and 55 patients (23%) with Pa bacteraemia. Treatment failure was significantly higher in CR-Ab than in CR-Pa (60.6% vs. 34.5%, P = 0.001). In Ab, severe sepsis or septic shock and high Pitt bacteraemia score were independent risk factors for treatment failure in the inappropriate empirical antibiotics group. In Pa, hospital-acquired infection and high Pitt bacteraemia score were independent risk factors for treatment failure in both groups. CR was an independent risk factor in Ab for treatment failure in both groups, but not in Pa bacteraemia. We demonstrated significant differences in clinical characteristics and impact of CR on clinical outcomes between Ab and Pa bacteraemia, suggesting that different treatment approaches may be needed.
Multi-dimensional quantitative model of communication network value
Authors: Wu Lijuan , Gan Chaoqin , Xu Zhongsen , Hui Jianqiang 
Published: May 2022

AbstractIn this paper, a multi-dimensional quantitative model is firstly founded for evaluating communication network value, and the quantification of the abstract concept of network value is realized. By analyzing various factors that influence the evaluation of network value from multiple perspectives, an index system of multi-dimensional network value evaluation is established. By adopting the analytic hierarchy process (AHP) method, not only the weight of each dimension is reasonably determined, but also the weight of different indicators in the second dimension is determined. By the characteristics of each dimension and its weight in the evaluation system, a multi-dimensional quantitative model for evaluating communication network value is constructed. By analyzing the value of Ethernet passive optical network (EPON), the rationality of the proposed quantitative model is demonstrated.
Transmission dynamics of SARS-CoV-2 in a strictly-Orthodox Jewish community in the UK
Authors: Waites William , Pearson Carl A. B. , Gaskell Katherine M. , House Thomas , Pellis Lorenzo , Johnson Marina , Gould Victoria , Hunt Adam , Stone Neil R. H. , Kasstan Ben , Chantler Tracey , Lal Sham , Roberts Chrissy H. , Goldblatt David ,   CMMID COVID-19 Working Group, Abbas Kaja , Abbott Sam , Atkins Katherine E. , Barnard Rosanna C. , Bosse Nikos I. , Brady Oliver , Chan Yung-Wai Desmond , Chapman Lloyd A. C. , Clifford Samuel , Davies Nicholas G. , Edmunds W. John , Eggo Rosalind M. , Endo Akira , Finch Emilie , Flasche Stefan , Foss Anna M. , Funk Sebastian , Gibbs Hamish P. , Gimma Amy , Hellewell Joel , Hodgson David , Hué Stéphane , Jafari Yalda , Jarvis Christopher I. , Jit Mark , Jombart Thibaut , Klepac Petra , Knight Gwenan M. , Koltai Mihaly , Krauer Fabienne , Kucharski Adam J. , Liu Yang , Lowe Rachel , McCarthy Ciara V. , Meakin Sophie R. , Medley Graham , Mee Paul , Munday James D. , Nightingale Emily S. , O’Reilly Kathleen , Pearson Carl A. B. , Prem Kiesha , Procter Simon R. , Pung Rachael , Quaife Matthew , Quilty Billy J. , Rosello Alicia , Russell Timothy W. , Sandmann Frank G. , Sun Fiona Yueqian , Tully Damien C. , Villabona-Arenas C. Julian , Waites William , Waterlow Naomi R. , Wong Kerry L. M. , van Zandvoort Kevin , Marks Michael , Eggo Rosalind M. 
Published: May 2022

AbstractSome social settings such as households and workplaces, have been identified as high risk for SARS-CoV-2 transmission. Identifying and quantifying the importance of these settings is critical for designing interventions. A tightly-knit religious community in the UK experienced a very large COVID-19 epidemic in 2020, reaching 64.3% seroprevalence within 10 months, and we surveyed this community both for serological status and individual-level attendance at particular settings. Using these data, and a network model of people and places represented as a stochastic graph rewriting system, we estimated the relative contribution of transmission in households, schools and religious institutions to the epidemic, and the relative risk of infection in each of these settings. All congregate settings were important for transmission, with some such as primary schools and places of worship having a higher share of transmission than others. We found that the model needed a higher general-community transmission rate for women (3.3-fold), and lower susceptibility to infection in children to recreate the observed serological data. The precise share of transmission in each place was related to assumptions about the internal structure of those places. Identification of key settings of transmission can allow public health interventions to be targeted at these locations.
Corticotropin releasing hormone as an identifier of bronchiolitis obliterans syndrome
Authors: Niroomand Anna , Ghaidan Haider , Hallgren Oskar , Hansson Lennart , Larsson Hillevi , Wagner Darcy , Mackova Martina , Halloran Kieran , Hyllén Snejana , Lindstedt Sandra 
Published: May 2022

AbstractLung transplantion (LTx) recipients have low long-term survival and a high incidence of bronchiolitis obliterans syndrome (BOS), an inflammation of the small airways in chronic rejection of a lung allograft. There is great clinical need for a minimally invasive biomarker of BOS. Here, 644 different proteins were analyzed to detect biomarkers that distinguish BOS grade 0 from grades 1–3. The plasma of 46 double lung transplant patients was analyzed for proteins using a high-component, multiplex immunoassay that enables analysis of protein biomarkers. Proximity Extension Assay (PEA) consists of antibody probe pairs which bind to targets. The resulting polymerase chain reaction (PCR) reporter sequence can be quantified by real-time PCR. Samples were collected at baseline and 1-year post transplantation. Enzyme-linked immunosorbent assay (ELISA) was used to validate the findings of the PEA analysis across both time points and microarray datasets from other lung transplantation centers demonstrated the same findings. Significant decreases in the plasma protein levels of CRH, FERC2, IL-20RA, TNFB, and IGSF3 and an increase in MMP-9 and CTSL1 were seen in patients who developed BOS compared to those who did not. In this study, CRH is presented as a novel potential biomarker in the progression of disease because of its decreased levels in patients across all BOS grades. Additionally, biomarkers involving the remodeling of the extracellular matrix (ECM), such as MMP-9 and CTSL1, were increased in BOS patients.
Klotho expression in peripheral blood circulating cells is associated with vascular and systemic inflammation in atherosclerotic vascular disease
Authors: Martín-Núñez Ernesto , Pérez-Castro Atteneri , Tagua Víctor G. , Hernández-Carballo Carolina , Ferri Carla , Pérez-Delgado Nayra , Rodríguez-Ramos Sergio , Cerro-López Purificación , López-Castillo Ángel , Delgado-Molinos Alejandro , López-Tarruella Victoria Castro , Arévalo-Gómez Miguel A. , González-Luis Ainhoa , Martín-Olivera Alberto , Morales-Estévez Carmen Chaxiraxi , Mora-Fernández Carmen , Donate-Correa Javier , Navarro-González Juan F. 
Published: May 2022

AbstractCardiovascular disease is the leading cause of death worldwide. New therapeutic strategies are aimed to modulate the athero-inflammatory process that partially orchestrates underlying vascular damage. Peripheral blood circulating cells include different immune cells with a central role in the development of the atherogenic inflammatory response. The anti-aging protein α-Klotho has been related to protective effects against CVD. KL is expressed in monocytes, macrophages, and lymphocytes where it exerts anti-inflammatory effects. In this work, we analyse the relationships of the levels of inflammatory markers with the expression of the KL gene in PBCCs and with the serum levels of soluble KL in atherosclerotic vascular disease. For this, we conducted a cross-sectional single-center case–control study including a study group of 76 CVD patients and a control group of 16 cadaveric organ donors without medical antecedent or study indicating CVD. Vascular artery fragments and whole blood and serum samples were obtained during elective or organ retrieval surgery. Serum levels of sKL, TNFα and IL10, and gene expression levels of KL, TNF, IL10, NFKB1, DNMT1, and DNMT3A in PBCCs were measured. In these cells, we also determined KL promoter methylation percentage. Histological and immunohistochemical analyses were employed to visualize atherosclerotic lesions and to measure IL10 and TNFα levels in vascular fragments. Patients with CVD presented higher values of proinflammatory markers both at systemic and in the vasculature and in the PBCCs, compared to the control group. In PBCCs, CVD patients also presented lower gene expression levels of KL gene (56.4% difference, P < 0.001), higher gene expression levels of DNMT1 and DNMT3A (P < 0.0001, for both) and a higher methylation status of in the promoter region of KL (34.1 ± 4.1% vs. 14.6 ± 3.4%, P < 0.01). In PBCCs and vasculature, KL gene expression correlated inversely with pro-inflammatory markers and directly with anti-inflammatory markers. sKL serum levels presented similar associations with the expression levels of pro- and anti-inflammatory markers in PBCCs. The differences in KL expression levels in PBCCs and in serum sKL levels with respect to control group was even greater in those CVD patients with macroscopically observable atheromatous plaques. We conclude that promoter methylation-mediated downregulation of KL gene expression in PBCCs is associated with the pro-inflammatory status in atherosclerotic vascular disease.
Women in gig economy work less in the evenings
Authors: Dokuka Sofia , Kapuza Anastasia , Sverdlov Mikhail , Yalov Timofey 
Published: May 2022

AbstractWomen have been systematically disadvantaged in the labour market. This could be explained by a complex association of factors, such as the lower speed of women’s professional growth within companies, their under-representation in management positions, and the unequal distribution of caregiving and housework between men and women. The rise of the gig economy—a market system that is based on hiring independent contractors and freelance workers as opposed to creating full-time contracts—has brought researchers and policymakers into a discussion on the effects of online platforms and flexible work arrangements on labour market gender parity. In this study, we examine the case of the largest online English-language school in Eastern Europe, Skyeng. Data on 6,461,404 lessons given by 13,571 teachers demonstrate that women had fewer working hours than men in most age categories, but especially for ages 30–35. The workload deficit for the women could be partly attributed to the fact that they worked less often than the men did in the evenings (7–10 p.m.). We conclude that, despite the flexible work arrangements the gig economy has offered, the women taught fewer classes than the men (i.e., having fewer paid working hours), which in turn led to a gender pay gap. The rapid growth of the gig economy makes it important to monitor gender-gap dynamics as well as discuss potential mechanisms eliminating gender inequality in the labour market.
Pilot study of factors contributing to canine impaction after secondary alveolar bone grafting in unilateral cleft lip and palate patients
Authors: Tsurumi Yoko , Nishimura Kazuaki , Shimizu Megumi , Imai Yoshimichi , Igarashi Kaoru 
Published: May 2022

AbstractAlveolar bone grafting (ABG) is broadly performed for cleft lip and palate patients. The rate of canine impaction post-ABG is much higher than the prevalence of canine impaction in non-cleft patients. This pilot study was designed to investigate factors involved in canine eruption failure after ABG and to predict the possibility of canine impaction in unilateral cleft lip and palate (UCLP) patients. This retrospective observational study examined 45 patients with UCLP (mean age 7.9 years) classified into an impacted group (n = 9) and a spontaneously erupted group (n = 36). From 3D images, we measured lateral incisor presence or absence, lateral incisor position on the cleft side, canine position, movement change, cleft volume, and canine and first premolar overlap-area. Multivariate logistic regression analysis using independent variables indicated significant differences in results, selecting highly relevant items. Multivariate analysis indicated a significant association between the overlap-area between the canine and the first premolar at pre-ABG (p = 0.038) and the distance between the cleft side cusp tips of canine and the lateral cleft margin of pre-ABG (p = 0.005). Results suggest that canine impaction is predictable at an early stage in pre-ABG and show the possibility of comprehensive diagnosis of canine impaction using computed tomography.
Super-resolution visualization of chromatin loop folding in human lymphoblastoid cells using interferometric photoactivated localization microscopy
Authors: Parteka-Tojek Zofia , Zhu Jacqueline Jufen , Lee Byoungkoo , Jodkowska Karolina , Wang Ping , Aaron Jesse , Chew Teng-Leong , Banecki Krzysztof , Plewczynski Dariusz , Ruan Yijun 
Published: May 2022

AbstractThe three-dimensional (3D) genome structure plays a fundamental role in gene regulation and cellular functions. Recent studies in 3D genomics inferred the very basic functional chromatin folding structures known as chromatin loops, the long-range chromatin interactions that are mediated by protein factors and dynamically extruded by cohesin. We combined the use of FISH staining of a very short (33 kb) chromatin fragment, interferometric photoactivated localization microscopy (iPALM), and traveling salesman problem-based heuristic loop reconstruction algorithm from an image of the one of the strongest CTCF-mediated chromatin loops in human lymphoblastoid cells. In total, we have generated thirteen good quality images of the target chromatin region with 2–22 nm oligo probe localization precision. We visualized the shape of the single chromatin loops with unprecedented genomic resolution which allowed us to study the structural heterogeneity of chromatin looping. We were able to compare the physical distance maps from all reconstructed image-driven computational models with contact frequencies observed by ChIA-PET and Hi-C genomic-driven methods to examine the concordance between single cell imaging and population based genomic data.