bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decreased pregnancy rate per embryo transfer in women undergoing assisted reproductive technology after abdominal trachelectomy: A retrospective study
Authors: Fujita Tomoyuki , Yoshioka Naomi , Ogawa Shoko , Furukawa Yuichi , Otsuka Misako , Kuramoto Takeshi 
Published: October 2022

Abstract
Purpose: To evaluate the assisted reproductive technology (ART) outcomes after abdominal trachelectomy (AT).
Methods: This was a retrospective study. We compared the ART outcome of 13 patients who underwent AT at another hospital prior to undergoing ART at our clinic after surgery (T group) and 52 control patients selected based on age, the time of treatment onset, and anti-Müllerian hormone levels (non-T group).
Results: In all oocyte retrieval cycles, there were no significant differences in the number of oocyte retrieval, rate of fertilization, and good blastocysts. The pregnancy and live birth rates per embryo transfer (ET) were lower in the T group than in the non-T group (pregnancy rate, 20% versus [vs.] 39%, p=0.0166; live birth rate, 15% vs. 30%, p=0.0281). Endometrial thickness before ET was lesser in the T group than in the non-T group (7.4 [minimum–maximum, 3.5–14.3] mm vs. 9.0 [5.5–14.9] mm, p<0.0001). Multivariate logistic regression models showed that the age at oocyte retrieval (adjusted odds ratio [OR], 0.78; 95% confidence interval [CI], 0.69–0.89), the presence of good embryos (adjusted OR, 3.17; 95% CI, 1.29–7.82), and a history of AT (adjusted OR, 0.35; 95% CI, 0.15–0.83) were factors related to pregnancy per ET.
Conclusion: The pregnancy rate per ET was lower and the endometrium was thinner before ET in patients with than in those without a history of AT. It is important for clinicians to be aware of the longer time to pregnancy in patients on ART after AT.
The effects of short-term heat stress on the activity of protective enzymes and total antioxidant capacity in Tetranychus urticae (Acari: Tetranychidae)
Authors: Nie Peng-Cheng , Yang Ruo-Lan , Yuan Shao , Zhou Jing-Jiang , Shang Su-Qin 
Published: October 2022

Abstract
Tetranychus urticae Koch is a worldwide pest mite which can cause continuous damage to multiple plants even in hot environment such as greenhouse due to its strong endurance to high temperature. In order to clarify the physiological mechanisms of T. urticae in response to short-term heat stress, four high temperatures (36, 39, 42, and 45°C), three short-term heat durations (2, 4, and 6 h) were conducted to test the effects on the protein content of T. urticae and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and total antioxidant capacity (T-AOC), respectively. The results showed that protein content, antioxidant enzymes activity, and T-AOC in T. urticae were significantly affected by both the high temperature and duration after treatment, meanwhile, they showed same trends of increased at 25–39℃, then decreased at 39–42℃ except for CAT activity. The highest values of SOD activity, POD activity and T-AOC were all obtained at 39℃-4h, and that of CAT activity was obtained at 39℃-2h. The higher levels of T-AOC serve an important signal demonstrating that T. urticae has other antioxidant mechanisms except for antioxidant enzymes. Those results suggested that heat stress induces oxidative stress, and antioxidant enzymes played important role in reducing oxidative damage in T. urticae. which provided a basis for further research on the molecular mechanisms of thermostability and ecological adaptability of T. urticae.
An individual-based spatial epidemiological model for the spread of plant diseases
Authors: Cendoya Martina , Navarro-Quiles Ana , López-Quílez Antonio , Vicent Antonio , Conesa David 
Published: October 2022

Abstract
In the study of plant disease epidemics, there is often an interest in the state at the individual level to analyze disease progression, where the spatial structure of the population should be considered. Based on the disease status of the individuals, we developed a model to simulate plant disease spread in time and space, where the spatial dependence was introduced through the Matérn correlation function. Infection of a susceptible individual depended on the force of infection, given by the transmission rate of infected individuals and the spatial correlation. The algorithm for disease spread was implemented in Python, which allowed simulations of large populations to be handled efficiently. Using almond leaf scorch disease, caused by the bacterium Xylella fastidiosa, as a case study, the behavior of the model parameters and the type of initial introduction (random or aggregated) on disease spread was tested by simulation, as well as the effect of introducing and/or removing infected individuals. The greatest variability in results depended on the range parameter of the Matérn correlation function and type of initial introduction. In areas with high population density, the spread rate was almost constant, while regions without trees acted as a barrier to spread when the scope of their extent was greater than the value of the range parameter. This individual-based model can be also applied to other plant diseases, adapting the parameter values to their particular epidemiological characteristics.
Response of microbial community in the soil of halophyte after contamination with tetrabromobisphenol A
Authors: Gu Chen , Zhang Fan , Lu Kaiwen , Sun Xiaoqing , Guo Wenrui , Shao Qing 
Published: October 2022

Abstract
Coastal wetlands are subjected to increasing tetrabromobisphenol A (TBBPA) pollution, whereas knowledge on TBBPA degradation in marine environments is lacking. The changes of bacterial communities in TBBPA-polluted soil covered with halophytes were investigated. TBBPA could be degraded in the halophyte covered saline-alkali soil in microcosm experiment. Higher TBBPA removal occurred in the soil of Kandelia obovata compared with soils covered with Suaeda australis and Phragmites australis within 56 days of cultivation. The rhizosphere soils of S. australis, P. australis, and K. obovata mainly involved the classes of Bacteroidia, Gammaproteobacteria, Alphaproteobacteria, and Anaerolineae. Additionally, manganese oxidation, aerobic anoxygenic phototrophy, and fermentation funcitions were higher in the rhizosphere soil of K. obovata after TBBPA addition. This study indicated that the vegetation type is a vital factor influencing the biodegradation mechanism of TBBPA and other related organic pollutants in salt marsh ecosystem.
Impact of the Tambora Volcanic Eruption of 1815 on Islands and Relevance to Future Sunlight-Blocking Catastrophes
Authors: Wilson Nick , Cassidy Michael , Boyd Matt , Mani Lara , Valler Veronika , Brönnimann Stefan 
Published: October 2022

Abstract
Island nations may have potential long-term survival value for humanity in global catastrophes such as sun-blocking catastrophes from nuclear winter and large magnitude volcanic eruptions. One way to explore this issue further is to understand the impact on islands after the largest historically observed volcanic eruption: that of Mt Tambora in 1815.
For each of the 31 large populated islands selected, we conducted literature searches for relevant historical and palaeoclimate studies. We also analysed results from a reconstruction (EKF400v2), which uses atmospheric-only general circulation model simulations with assimilated observational and proxy data. From the literature review, in those islands with data, there was evidence for weather/climate anomalies in 1815-1817 for all of them (100%; 29/29), impaired food production in 67% (8/12), and food insecurity/famine in 33% (4/12). Based on the EKF400v2 reconstruction for temperature anomalies (compared to the relatively “non-volcanic” reference period of 1779 to 1808), all but four islands showed statistically anomalous temperature reductions in the 1816-1817 period (for most p<0.00001). In the peak impact year of 1816, the lowest anomalies were seen for islands in the Southern Hemisphere (p<0.0001), the Indian Ocean (p<0.0001), and in the tropics and subtropics of the Southern Hemisphere (p=0.0057). In conclusion, the findings of both the literature review and reconstruction simulations suggest climatic impacts of the Tambora eruption for nearly all these 31 large islands. But islands in the Southern Hemisphere, in particular the Indian Ocean and the tropics and subtropics of the Southern Hemisphere, experienced relatively smaller temperature anomalies.
Female Gender was a Protective Factor for Patients with Frailty Undergoing Total Knee or Hip Replacement
Authors: Lian Qiang , Lian Yun , Fu Jinlang , Gan Wei , Yang Qinfeng , Wang Jian 
Published: October 2022

Abstract
Background
Women reported significantly poorer health and poorer outcomes in some surgeries than men. As the patients with frailty got more and more attention in joint placement. It is necessary to find whether frail women also need to be paid more attention after TKA or THA. Further, it is also needed to discuss specifically the different impacts of sex in the perioperative period for frail patients undergoing TKA or THA.
Method
We used the frailty-defining diagnosis cluster and the discharge data from the National Inpatient Sample database of patients who underwent THA and TKA. Bivariate and multivariate analysis methods were performed to find the association between sex and patient characteristics and postoperative complications of these patients.
Result
Frail patients undergoing TKA comprised 34.6% male and 65.4% female. In the multivariate analysis, the female sex was found to be a protective factor for mortality, acute cardiac events, acute renal failure, pneumonia, DVT_PE, and postoperative delirium. Frail patients undergoing THA were 39.5% male and 60.4% female. In the multivariate analysis, the female sex was found to be a protective factor for acute cardiac events, acute pulmonary edema, acute renal failure, and pneumonia. Moreover, whether in TKA or THA, the male sex rather than the female sex is closely associated with serious comorbidities. Lower hospital costs were also associated with the female sex.
Conclusion
Female sex acts as a protective factor for postoperative complications of THA or TKA. Therefore, frail men rather than frail women need more attention from clinicians, although the proportion of frail women is far greater than that of men.
Long-chain non-coding RNA MALAT1 upregulates the MMP-2 signaling pathway by inhibiting miR-106a-5p in murine oxygen- induced retinopathy
Authors: Li Zhaojin , Li Kunyue , Mu Siyuan , Wang Zhibin , Di Yu 
Published: October 2022

Abstract
Retinopathy of prematurity (ROP) is a blinding eye disease in children that is characterized by the formation of neovascularization in the retina; current treatments for this disease risk retinal damage and visual impairment. This study aimed to investigate the relationship between metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), miR-106a-5p, and matrix metalloproteinase 2 (MMP-2) in an oxygen-induced retinopathy mouse model and hypoxia-induced human retinal endothelial cells and to elucidate whether MALAT1 upregulates MMP-2 signaling by inhibiting miR-106a-5p. The role of this pathway in oxygen-induced murine retinopathy and its underlying mechanism were also investigated. MALAT1 inhibited the expression of miR-106a-5p and enhanced the expression of MMP-2, which in turn caused a series of pathological changes, such as the formation of new blood vessels in the retina. In addition, knockdown of MALAT1 can downregulate the expression of MMP-2 by sponging miR-106a-5p and inhibiting cell proliferation, migration, and tube-forming ability. In conclusion, our findings suggest that MALAT1 may contribute to the occurrence and development of ROP by inhibiting miR-106a-5p and increasing the expression of MMP-2, thus providing a new perspective for the targeted therapy of ROP.
Development and validation of bead-based assay quantifying Tetanus, Diphtheria, Pertussis Toxin, Filamentous haemagglutinin and Pertactin specific IgG in human serum
Authors: Rathod Vishal , Kadam Laxmikant , Gumma Prabhu Dasu , Gautam Manish , Sharma Hitt , Shaligram Umesh , Rao Harish , Gairola Sunil , Parekh Sameer , Marke Kevin , Asokanathan Cathy , Douglas-Bardsley Alex , Hassell Laura 
Published: October 2022

Abstract
Conventional ELISA platforms have been used for vaccine immunogenicity testing. However, due to limitations in sourcing and accessibility to human serum samples, we report the development and validation of Luminex-based multiplex immunoassay (MIA), using monovalent beads, which would reduce the analysis time, cost, and sample volume while simultaneously measuring the concentration of serum immunoglobulin G (IgG) antibodies specific for tetanus (TT), diphtheria (DT), pertussis toxin (PT), filamentous hemagglutinin (FHA) and pertactin (PRN), using the NIBSC reference standards. Additionally, we also report the development of a multiplex reference standard (MRS) focused on the simultaneous evaluation of antibodies against T, D, PT, PRN, and FHA in healthy human sera samples. As an assay evaluation parameter, the precision, accuracy, dilutional linearity, minimum and maximum detectable limit, robustness, stability, etc were assessed. The assay exhibited a wide dynamic range for all the five antigens and could quantify the IgG concentrations down to minimum concentrations, demonstrating antigen specificity with no cross-talks among the beads. The results obtained with MIA were consistent with commercially available assays. Thus, to conclude, the study provided a pentaplex assay with increased sensitivity, reproducibility and high throughput capabilities which would allow design of large and robust clinical studies for evaluating natural and vaccine-induced immunity.
Soil properties and plant species can predict population health and potential introduction sites of the endangered orchid Cypripedium calceolus
Authors: Rusconi Olivia , Steiner Théo , Bayon Claire Le , Rasmann Sergio 
Published: October 2022

Abstract
Background and Aims – To counteract the ongoing worldwide biodiversity loss, conservation actions are required to re-establish and regenerate populations of threatened species. Two key factors predominantly involved in finding the most suitable habitats for endangered plant species are the surrounding plant community composition and the physicochemical parameters of the soil rooting zone. However, such factors are likely to be context- and species-dependent, so it remains unclear to what extent they influence the performance of the target species.
Methods – Here, we studied 34 Swiss populations of the highly endangered orchid Cypripedium calceolus. We measured functional traits related to plant and population performance, realized vegetation surveys, and soil profiles analyses, and tested for relationships between plant traits and the surrounding vegetation structure or soil physicochemical parameters.
Results – We found that neither vegetation alliances nor soil classes per se could predict C. calceolus functional traits and population size. However, we found that C. calceolus functional traits explaining population performance and size were related to specific soil parameters, namely soil organic matter content, pH and phosphorus, in addition to a combination of presence-absence of six plant indicator species, relating to ecotones between forests and forest gaps.
Conclusion – We show that, even for species that can grow across a wide range of vegetation groups, such as the Lady’s Slippers orchids, both indicator species and specific soil parameters can be used to assess the optimal sites to implement (re)-introduction actions.
Correlation study of NF-κB, IER3, and recurrence of ovarian endometrioid cysts
Authors: Yang Ni , Huang Gu , Fan Xiang , Zhang Chao , Wang Li , Jiang Xian , Fan Gang , Zhang Lina , Zhu Pengfeng 
Published: October 2022

Abstract
Objective
To investigate the expression of nuclear actor-k-gene binding(NF-κB) and immediate early response 3(IER3) in nuclear factor-activated B cells in ovarian endometrioid cysts. To analyze the correlation between the expression of NF-κB and IER3 and the recurrence of the ovarian endometrioid cyst.
Methods
From January 2018 to March 2019, a total of 80 patients who underwent laparoscopic ovarian cyst excision due to ovarian endometrioid cyst in the Department of Gynecology, Changzhou Maternal and Child Health Hospital were selected, including 40 patients without postoperative recurrence and 40 patients with recurrence, and the patients with recurrence were further divided into the primary recurrence and the secondary recurrence groups. The general clinical data of the patients were collected. The patient's Revised American Fertility Society (R-AFS) score, least function(LF) score, and endometriosis fertility index (EFI) were calculated, and immunohistochemical staining was performed to detect the expression of IER3 and NF-κB in the pathological tissues of the three groups. The receiver operating characteristic (ROC) curve was used to evaluate the predictive value of IER3 and NF-κB expression on postoperative recurrence of the ovarian endometrioid cyst. Cox proportional hazards model was fitted to analyze the related influencing factors of ovarian endometrioid cyst recurrence.
Results
The expression intensity of NF-κB was positively correlated with IER3 (P < 0.001). ROC curve showed that the area under curve (AUC) of NF-κB and IER3 combined diagnosis was 0.844, 95%CI was 0.754–0.933, sensitivity was 77.5% and specificity was 90.00%. Multivariate Cox survival regression showed that the IER3 expression intensity > 4.5 (HR = 4.661,95%CI: 1.712–12.690, P = 0.003) and the NF-κBexpression intensity > 4.5 (HR = 2.878,95%CI: 1.181–7.011, P = 0.020) were independent risk factor for postoperative recurrence, and EFI score (HR = 1.289,95%CI: 1.037–1.602, P = 0.022) was a protective factor for postoperative recurrence.
Conclusion
EFI score is a protective factor for postoperative recurrence, and the expression intensity of NF-κB and IER3 has a certain correlation with the recurrence of ovarian endometrioid cysts. The expression levels of NF-κB and IER3 > 4.5 are independent risk factors for postoperative recurrence.
Impact of hospital volume on mortality and cost in sepsis based on a Japanese nationwide medical claims database study
Authors: Oami Takehiko , Imaeda Taro , Nakada Taka‑aki , Aizimu Tuerxun , Takahashi Nozomi , Abe Toshikazu , Yamao Yasuo , Nakagawa Satoshi , Ogura Hiroshi , Shime Nobuaki , Umemura Yutaka , Matsushima Asako , Fushimi Kiyohide 
Published: October 2022

Abstract
We aimed to investigate the impact of hospital volume on the mortality rate and medical costs in sepsis. This retrospective study used the administrative data from 2010 to 2017. The hospital volume categorization into quartiles was performed according to the annual number of sepsis cases. The primary and secondary outcome was in-hospital mortality and medical costs, respectively. A mixed-effects logistic model with a two-level hierarchical structure was used to adjust for baseline imbalances. Among 1,781,014 sepsis patients from 1,622 hospitals, the crude in-hospital mortality rate was 22.1% and 17.0% in the lowest and highest quartile of sepsis volume, respectively (p < 0.0001). After adjustment for confounding factors, the in-hospital mortality in the highest quartile was significantly lower than that of the lowest quartile (odds ratio, 0.789; 95% confidence interval, 0.774–0.804). Investigations with fractional polynomials and restricted cubic splines revealed that the hospital volume was significantly associated with in-hospital mortality (p < 0.0001). The highest quartile had higher daily medical costs per person than those of the lowest quartile. In conclusion, this nationwide sepsis study using the medical claims database suggested that a higher hospital volume leads to lower in-hospital mortality and higher daily medical costs per person.
Knowledge and Attitude of Dietary Supplements Among Arab Olympic Athletes and Coaches in Preparation Program for TOKYO 2020 Olympic Games
Authors: Amawi Adam Tawfiq , Moualla Dania Sameer , Alshuwaier Ghareeb O. , Alaqil Abdulrahman , Nemer Lana Salah Subhi 
Published: October 2022

Abstract
Background: Nutrition plays an important role as a key factor in the performance of athletes and their coaches, so good and proper nutrition is essential for improving athletic performance and their physical fitness. The lack of nutritional knowledge of athletes may affect their performance, and the most important of these issues is nutritional knowledge of food and dietary supplements
Aim: To assess the nutritional knowledge, attitude, and practice of athletes and their coaches at the Arab Olympic Preparation Center about the dietary supplements, the Knowledge, Attitude and Practice Strategy has been used to assess this.
Mothed: A questionnaire-based study was applied to a convenient sample of 111 elite athletes and coaches in Arab Olympic preparation program during the period between January and March 2020 from different sport types. The questionnaire contained two-parts (personal information and nutritional supplement knowledge, attitude, and practice questionnaire) to meet the purpose of the study.
Results: The total percentage of athletes’ knowledge about nutritional supplements is only 46.94 %, indicating that this important group of society does not have sufficient knowledge of nutritional supplements. While the percentage of use of dietary supplementsamong elite athletes was approximately 41.6%, which is considered to be relatively high. Also, our study showed that coaches had positive impact on the elite athletes due to good coaches' knowledge, 84.2 % of elite athletes indicated that they had taken information from their coaches about dietary supplements, and 53.5 % of elite athletes indicated that they had participated in nutritional supplement workshops.
Conclusion: Based in our study, there was a relatively high prevalence of dietary supplements use among elite athletes and coaches at the Arab Olympic Preparation Program due to lack of knowledge about dietary supplements.
Too Much Finance and Top Income Concentration: A Dynamic Panel Data Analysis
Authors: Siddique Md. Abu Bakkar , LEE Kang-Kook 
Published: October 2022

Abstract
This study investigates the effect of financial development on top income concentration and income inequality. We conducted a dynamic panel estimation using the GMM method for 171 countries, including developed and developing economies, over 1970–2017. Using linear regression, we find that financial development has a linear positive and significant effect on the top 1% and top 10% income share. In addition, nonlinear regression results report that private credit has a U-shape effect on top income concentration. This suggests that too much finance is beneficial for the top income group but detrimental to income distribution for the entire population.
JEL Codes: E44, E24, E51
Whole exome sequencing provides a diagnosis of spinal muscular atrophy pedigree with SMN1 “2+0” genotype
Authors: Zhang Xiaoli , Gao Jinshuang , Guo Yaqing , Li Xiao , Zhang Chenyu , Wang Mengyue , Yu Haiyang , Yuan Erfeng , Li Xiaoli , Chen Hao , Guan Jing , Jia Tianming , Zhang Linlin 
Published: October 2022

Abstract
Background
Spinal muscular atrophy (SMA) is one of the common autosomal recessive neuromuscular disease caused by mutations of the SMN1 gene. As a special SMA carrier, the “2 + 0” genotype of SMN1 poses a great challenge for carrier screening and family genetic counseling.
Methods
In this study, for the first time, we identified “2 + 0” genotype carriers via trio-based whole exome sequencing (WES) and sequencing based multiple single nucleotide polymorphisms (SNPs) haplotype linkage analysis.
Results
Combined with the copy number of SMN1 gene in family members, the genetic relationship of SMN1 pathogenic gene transmitted from grandmother to father and then to proband was confirmed.
Conclusion
Our study suggest that SNPs transmitted through multiple generations in a family could be screened by using WES sequencing to realize linkage analysis. And we determine that the SMN1 genotypes of proband’s father and grandmother are “2 + 0” genotype carriers.