bluuebunny/crossref_metadata_2025_split huggingface dataset:
Higher systolic blood pressure is specifically associated with better islet beta-cell function in T2DM patients with high glycemic level
Authors: Xia Zhang , Song Lijuan , Fang Dongdong , You Wenjun , Li Feng , Zheng Deqiang , Li Yuhao , Lin Lu , Dou Jingtao , Su Xin , Zhai Qi , Zuo Yingting , Zhang Yibo , Gaisano Herbert Y. , Jiang Jiajia , He Yan 
Published: October 2022

Abstract
Background
Patients with type 2 diabetes mellitus (T2DM) usually have high blood viscosity, which could decrease blood supply to the pancreas. Increased blood pressure (BP) has been reported as a potentially compensatory response to the maintenance of islet blood perfusion. However, the influence of BP on beta-cell function in people with diabetes remains inconsistent. This study aimed to examine the relationship between BP and beta-cell function in patients with T2DM under different HbA1c levels.
Methods
This cross-sectional study included 615 T2DM patients. Clinical data were extracted from hospital medical records. Beta-cell function was assessed with insulin secretion-sensitivity index-2 (ISSI2). Multivariable linear regression analysis and restricted cubic splines (RCS) analysis were performed to identify the association between systolic blood pressure (SBP) and ISSI2. Mediation analysis was performed to determine whether higher SBP could reduce blood glucose by enhancing beta-cell function.
Results
After adjustment of age, sex, duration of diabetes, blood lipids, Matsuda index, and drug treatment, in participants with HbA1c ≥10%, SBP was positively associated with log ISSI2 (b = 0.044, 95%CI: 0.011, 0.078), and the SBP between 140 to150 mmHg had the highest log ISSI2 (b = 0.306, 95%CI: 0.126, 0.485). RCS analyses further demonstrated an inverted U-shaped association between SBP and ISSI2 with the SBP at 146 mmHg corresponding to the best beta-cell function. Additionally, higher SBP was “paradoxically” associated with lower 2h postprandial blood glucose (PBG) when SBP < 150mmHg, which was almost exclusively mediated by ISSI2 (mediating effect=-0.057, 95%CI: -0.085, -0.029). However, SBP was not associated with ISSI2 and 2h PBG in participants with HbA1c < 10%.
Conclusions
A certain degree of elevated SBP (140–150 mmHg) was associated with better beta-cell function and reduced 2h PBG in T2DM patients with HbA1c ≥10% but not in those with HbA1c < 10%. T2DM patients with unfavorable glycemic control may benefit from higher SBP through enhanced islet beta-cell function.
Design of Peptides for Inhibiting Amyloidogenesis by Blocking Hydrophobic Interactions
Authors: Saiki Masatoshi , Shibatate Ikumi 
Published: October 2022

Abstract
Amyloid fibrils, formed by the mis-assembly of proteins with abnormal conformations, are responsible for several neurodegenerative disorders, including prion disease. To prevent amyloid formation, it is necessary to block aggregation of the stem-forming regions of these proteins. Here we have designed and tested peptides for blocking β-sheet aggregation based on two fundamental criteria of amyloid formation: first, the hydrophobic interactions between residues on neighboring antiparallel β-strands are formed along a fibril axis; and second, the interacting hydrophobic residues are located on only one face of the β-strands. Blocking peptides designed with hydrophilic substitutions in the stem-forming regions of barnase 1–24 (BM1-24) were prepared by solid-phase peptide synthesis using Fmoc chemistry. Their effect on amyloid formation by BM1-24 and peptides of other amyloid-forming proteins was assessed by Thioflavin T (ThT) fluorescence assay and circular dichroism (CD) spectroscopy in the far-UV region. BM1-24 showed considerable amyloid formation, as indicated by a change in ThT fluorescence intensity (ΔF > 3). The addition of blocking peptides resulted in much lower fluorescence intesity (ΔF < 0.5), showing that amyloid formation was inhibited. CD spectroscopy suggested that the blocking peptide stabilized the β-structure of BM1-24. Addition of the blocking peptide to amyloid-forming peptides of prion, Amyloid β, and Pmel 17 led to a similar decline in ΔF, suggesting that amyloid formation was also inhibited in these proteins. Our results indicate that blocking peptides, designed according to the essential linear arrangement of hydrophobic residues, is effective at inhibiting amyloidogenesis in various types of proteins.
Chromogenic amide and CHEF-based emissive receptors for Cu 2+ &amp; Co 2+ , as well as biological anions including the notorious CN - : The impact of a molecular chameleon, enticing early career beginners
Authors: Amputu Martha , Naimhwaka Johannes , Uahengo Veikko 
Published: October 2022

Abstract

A rich-in-amide receptors, acrylamide-N’phenylhydrazine carbothioamide-based fluorescent probe (MA) was synthesized via a simple and one-step procedure of Schiff base condensation reaction. The structure was characterized via spectroscopic methods of 1H NMR, UV-Vis and fluorescence methods. In addition, the bench-top applications on the detection of cations and anions, in DMSO, were carried out visually and spectroscopically. As a result, MA displayed colorimetric activities once anions or cations were added, observable by naked eye detectable colour changes. The probe turned out to discriminate cations such as Cu2+, Co2+, Fe3+ and Fe2+ via colour changes, which was complemented by the changes in UV-Vis titration spectra for each individual titration. Moreover, the addition of anions (F-, AcO-, CN- and OH-) to MA had colorimetric impacts observable by naked eyes, and complemented by spectral changes upon UV-Vis analysis. The probe was fluorometrically responsive to the addition of cations (Co2+, Cu2+, Ni2+ and Fe2+) and anions (F-, AcO-, CN- and OH-), both in DMSO, which have all resulted in chelation-based fluorescence enhancement (CHEF), with Co2+ and AcO- complexes exhibiting high affinity of the host-guest species. Thus, MA proved to be a potential chromogenic-fluorogenic probe, which could be used in monitoring the presence of specific cations and anions, in aqueous soluble solvent of DMSO.
Cloud Security Game Theory Scoring From Predation Models In Simulation
Authors: Alsup Damon , Putluru Mohan , Cui Suxia , Zhang Yunpeng 
Published: October 2022

Abstract

Cloud economics results from amassing computation resources while being able to distribute workload in space and time. The backbone of this ability is virtualization, which abstracts the host hardware, sharing virtual machines. This means of interface is also a primary vehicle and target for attackers. The counter-measures to this threat consider the costs and benefits to the cloud’s essential functions. Where the future development of the cloud is also considered, this competition between attackers and victims is analogous to those modeled in extended game theory. Yet, the attacker and victim costs and benefits, expressed as utility measures necessary for game-theory methods, are elusive quantities. This paper establishes such a game as a predator-prey contest played out on a data-center environment. A set of contestant parameters are applied at the threshold of a viable model to the recognizable boundaries. Measurement of system health is extracted in relief with individual cost, benefit, and risk. An examination of metrics capable of validating extended interaction is developed. This establishes a baseline model for more elaborate expression of security in cloud economics.
Long noncoding RNA network for lncRNA-mRNA interactions throughout swine estrous cycle reveals developmental and hormonal regulations in reproductive tissues
Authors: Park Yoon-Been , Lim Byeonghwi , Kim Jun-Mo 
Published: October 2022

Abstract
Background
The mechanism of estrous cycles of pigs should be explored because their reproductive traits are useful for manipulating productivity and solving problems such as infertility. These estrous cycles should be elucidated to understand the complex interactions between various reproductive tissues (including the ovary, oviduct, and endometrium) and the complex range of hormone secretions during estrous cycles. Long non-coding RNAs (lncRNAs) regulate target genes at transcriptional, post-transcriptional, and post-translational regulation levels in various species. However, unlike mRNAs, lncRNAs in pigs have not been sufficiently annotated, and understanding at the protein level of coding genes has limitations in determining the mechanism of the reproductive traits of porcine. In this study, the lncRNAs of porcine ovary, oviduct, and endometrium were investigated on days 0, 3, 6, 9, 12, 15, and 18 of the estrous cycle. In addition, the characteristics and functions of the identified lncRNAs were explored.
Results
19,021 novel lncRNA transcripts were selected, and the comparison of the characteristics of the newly identified lncRNA and mRNA showed that similar to those of previous studies. Four lncRNA networks were chosen through network analysis. The cis-acting genes of lncRNAs included in each network were identified, and expression patterns were compared. The main lncRNAs (XLOC_021792, XLOC_017111, ENSSSCG00000050977, XLOC_000342, ENSSSCG00000050380, ENSSSCG00000045111, XLOC_008338, XLOC_004128, and ENSSSCG00000040267) were determined from the network by considering the cis-acting genes. Specific novel lncRNAs were discovered in the reproductive tissues during the swine estrous cycle, and their time-serial expression dynamics were confirmed.
Conclusions
As the main lncRNAs are involved in the development of each reproductive tissue and hormone action, they can be utilized as potential biomarkers to help improve and develop the reproductive traits of pigs.
Herbicides mixture with potent endocrine-disrupting properties in goldfish: As an early warning at environmentally relevant concentration.
Authors: Fatima Masroor , Rasool Fazle 
Published: October 2022

Abstract
For a meaningful interpretation of toxic effects in fish, knowledge of sub-lethal events is essential and for this reason, we have conducted a series of studies with a relevant environmental concentration of a mixture of herbicides; Atrazine, simazine, diuron and isoproturon (ASDI) pollutants and using immune (Fatima et al. 2007) and endocrinological changes as principal endpoints under controlled laboratory conditions. Goldfish (Carassius auratus) were exposed to a cumulative concentration of 50µg/l of ASDI mixture for 12 weeks which was reported above the permissible level in the drinking water of Belgium (EAEW report, 1998) and nearby aquatic environments, confirming some of these herbicides still persistence despite restriction policies (Pesce et al. 2010;Agbohessi et al. 2014).Control and exposed fish were sampled at week-4, 8, and 12. We investigated the endocrine modulatory effect of ASDI which shows a low degradation rate in water. Thus, at the realistic level of ASDI concentration, significantly increased levels of cortisol and aromatase after 4-weeks were recorded. Reduction in aromatase activity at 8 and 12 weeks is followed by estradiol (E2) showing a positive correlation between them. Hence, 11-Keto reduced significantly at 4-8 weeks and enhanced at 12 weeks. Remarkable reduction in E2 and 11-Keto may be correlated with the reduced egg production in stressed fish by these herbicides. However, no remarkable change in testosterone is observed at 4 and 12 weeks as compared to the control. ASDI mixture treatment induced remarkable induction in vitellogenin (Vtg) biomarker protein in male stressed fish. Furthermore, it may confer that modulation in the pathway of the reproductive cycle may alter the gamete quality, quantity and consequently may disrupt spawning and mating behaviour in fishes even at a realistic level of pollutant in the environment.
Climbing perch Anabas testudineus avoid expanded polystyrene pellets ingestion
Authors: Ganzha Ekaterina , Pavlov Efim , Duc Dien Tran 
Published: October 2022

Abstract

The climbing perch Anabas testudineus is widespread in the inland waters of Vietnam and according to its ecology could have contact with a floating plastic waste. The fragments of expanded polystyrene (EPS) detected into the fresh waters of Vietnam in Khanh Hoa, Lam Dong, Phu Yen provinces. Our study was focused on estimation of probability of ingestion of EPS pellets (size 2.5–3.5 mm) by adult climbing perch. In the experiments three types of treatment pellets were proposed to fish: 24 feed pellets (Fp), 24 expanded polystyrene pellets (Pp), 12 feed & 12 expanded polystyrene pellets (FPp). Fish grasping time of first pellet was independent in all treatment types. The time grasping of 12th pellet was insignificant in Fp (63 s) and Pp (75 s). Climbing perch was grasping and ingesting of 24th Fp significantly (p = 0.02) earlier (143 s), than grasping of 24th Pp (817 s). Fish with FPp treatment was grasping feed along with EPS pellets, but grasping of 12th Fp was significantly (p = 0.02) earlier (49 s), then 12th Pp (193 s). By the end of tests fish ingested all feed pellets. We discovered that climbing perch grasped Pp and kept them in oral cavity, but always rejected them in 100% cases. This result evidenced that climbing perch has effective defense mechanism avoiding ingestion of expanded polystyrene pellets with size 2.5–3.5 mm, which realized by taste system and tactile reception of fish.
The Set of All Natural Numbers Is Infinite and ω Can't Replace ∞
Authors: ZHANG Xin Fang 
Published: October 2022

Abstract
It is well known that Cantor believed that the set of all natural numbers was infinite and that ω could replace ∞. However, if we group all natural numbers and all pure decimals, we shall see that this is not the case. Indeed, by recursively defining x1=p1∈A1, xm=(pm,xm-1)∈Am, set Amm→ω contains all decimal numbers and clearly Amm→ω is finite. By recursively defining y1=q1∈B1, yn=(qn,yn-1)∈Bn, then Bnn→∞ contains all pure decimal in decimal. Therefore, when replacing ∞ with ω, Bnn→ω contains only all finite pure decimals. As a result, ω can not replace ∞.
Specific Profiles and Amplification of Resistance to Anti-tuberculosis Drugs in Tuberculosis Patients in the Republic of Karelia
Authors: Markelov Yu. M. , Belyaeva E. N. , Sunchalina T. V. 
Published: October 2022

The objective: to determine the frequency of resistance of Mycobacterium tuberculosis (MTB) to the most effective anti-tuberculosis drugs in tuberculosis patients in the Republic of Karelia, to characterize cases of amplification of MTB drug resistance during chemotherapy (CT).Subjects and Methods. MTB cultures of 1,137 tuberculosis patients were studied, the patients received chemotherapy from 2016 to 2021, among them 634 (55.8%) had multiple drug resistance (MDR). Among MBT cultures with MDR, there was a high frequency of MTB with extensive drug resistance (XDR) – 42.3% (268/634) including new cases. It provided a negative impact on the chemotherapy efficacy. Most often, amplification of MTB drug resistance was observed in patients treated as per regimen IV – 37/49 (75.5%) cases. MTB drug resistance amplified more often in the cases who were already resistant to 3 and more anti-tuberculosis drugs, 31/49 (63.3%). Most often, amplification was noted in aminoglycosides (Km, Am) and glycopeptide (Cm), (71.6% of cases). The high frequency of MTB drug resistance amplification from MDR up to XDR was due to the lack of rapid molecular genetic methods to test drug susceptibility to fluoroquinolones and aminoglycosides.
Association of Gln27Glu Polymorphism of the β2-adrenoreceptor Gene with Specific Parameters of the Immunoregulatory Cytokine Cascade and IgE Production in Patients with Bronchial Asthma
Authors: Turdibekov Kh. I. , Tashkenbaeva E. N. , Ziyadullaev Sh. Kh. , Ibragimov S. Kh. 
Published: October 2022

The objective: to study the level of serum cytokines IL-4, IFN-γ and the activity of IgE production in patients with bronchial asthma (BA) with the consideration of the Gln27Glu-polymorphism of the β2-AR gene.Subjects and Methods. 130 people of Uzbek ethnicity were examined including 83 patients with BA. BA patients were divided into groups according to the international classification of the World Health Organization and the GINA 2006 diagnostic criteria. Genotyping was performed by polymerase chain reaction technology and restriction fragment analysis.Results. In the group with allergic BA, patients with the Gln27Gln-polymorphic variant of the β2-adrenergic receptor gene had an average IgE value (1,664.20 ± 293.29 pg/ml) higher versus patients with the Gln27Glu genotype (338.40 ± 121.33 pg/ml; p < 0.01). No significant relationship between the Gln27Glu polymorphism of the β2-adrenergic receptor gene and the activity of inductor cytokines was found. The Gln27Gln genotype of the β2-adrenergic receptor gene in patients with bronchial asthma, especially in the allergic form of the disease, is associated with hyperproduction of IgE.
Features of protocols of assisted reproductive technologies in patients of the older age group depending on the ovarian stimulation drug: recombinant follicle-stimulating hormone or highly purified human menopausal gonadotropins
Authors: Kraevaya E. E. 
Published: October 2022

Introduction. An actual task of reproductive medicine is a comparative analysis of the use of highly purified urinary gonadotropins and recombinant FSH preparations for ovarian stimulation in in vitro fertilization programms in older patients. This was suggested on the basis of data available in the literature on the possible positive effect of LH on the effectiveness of various stages of ART programs in this category of women. Aim. To compare the parameters of ovarian stimulation, gametogenesis, early embryogenesis, pregnancy rate (PRF) in patients of older reproductive age in ART programs who underwent ovarian stimulation using drugs containing FSH + LH or drugs containing recombinant FSH. Materials and methods. The study included 44 patients aged 35 to 40 years with infertility due to tubal- peritoneal and non-severe male factor treated with ART techniques. In 50% of patients, ovarian stimulation was performed with highly purified urinary gonadotropin, in 50 % – with rFSH preparations. Results. The characteristics of the embryological stage in both groups showed high rates, comparable with global data. The frequency of pregnancy and early miscarriage was comparable in both groups. Conclusions. Ovarian stimulation with MHT is effective, safe and can be recommended for patients of older reproductive age.
Identifying Barriers and Enablers to Vaccine uptake from Immunizers and Individuals Receiving a COVID-19 Vaccine in Saskatchewan
Authors: Vanstone Jason Robert , Azizian Amir Reza , Berry Warren , Carr Tracey , Falastein Patrick , Mise Taysa-Rhea , Patel Shivani , Sarker Kinsuk Kalyan , Yasinian Maryam , Groot Gary 
Published: October 2022

Background:
Vaccine hesitancy presents a challenge to public health, especially during a global pandemic. Understanding reasons for vaccine hesitancy in local populations may help policymakers and public health practitioners increase vaccine uptake.

Objective:
We surveyed individuals receiving a COVID-19 vaccine and immunizers in Saskatchewan, Canada and categorized their responses according to the Theoretical Domains Framework. This provides policymakers with evidence-based suggestions for behaviour change interventions that may promote vaccine uptake among hesitant individuals.

Methods:
Two online surveys were developed to better understand vaccine hesitancy in Saskatchewan: one aimed at individuals receiving a vaccination and the other at immunizers. Both surveys were available for a one-week period when vaccination uptake had plateaued in Saskatchewan. Individuals receiving a vaccine were asked what made them decide to get a vaccine, and both groups were asked what they thought would promote vaccine uptake among hesitant individuals. Responses were analyzed thematically based on the Theoretical Domains Framework and reported descriptively.

Results:
Individuals receiving a COVID-19 vaccine indicated that mandates and restrictions and having a positive attitude toward COVID-19 vaccines were the most common reasons for receiving a vaccine. Immunizers most frequently indicated that media issues led to vaccine hesitancy and that having access to, and trust in, reputable information sources would enable more vaccine hesitant individuals to seek a COVID-19 vaccination.

Conclusion:
Mandates and restrictions, promoting positive attitudes towards vaccines, and ensuring people have access to, and trust in, reputable information sources, are key enablers for promoting vaccine uptake among vaccine hesitant individuals.
Impact of the Mobile Game FightHPV on Cervical Cancer Screening Attendance: Retrospective Cohort Study
Authors: Orumaa Madleen , Campbell Suzanne , Støer Nathalie C , Castle Philip E , Sen Sagar , Tropé Ameli , Adedimeji Adebola , Nygård Mari 
Published: October 2022

Background
The wide availability of mobile phones has made it easy to disseminate health-related information and make it accessible. With gamification, mobile apps can nudge people to make informed health choices, including attending cervical cancer screening.

Objective
This matched retrospective cohort study examined the association between exposure to the FightHPV mobile app gamified educational content and having a cervical exam in the following year.

Methods
Women aged 20 to 69 years who signed an electronic consent form after downloading the FightHPV app in 2017 (intervention group) were matched 1:6 with women of the same age and with the same screening history (reference group) in 2015. To estimate the impact of exposure to the FightHPV app, we estimated cumulative incidence and hazard ratios (HRs) with 95% CIs. We used data from the Norwegian Cervical Cancer Screening Program database and Statistics Norway to determine screening participation and outcomes, respectively.

Results
We matched 3860 women in the control group to 658 women in the intervention group; 6 months after enrollment, 29.6% (195/658) of the women in the intervention group and 15.21% (587/3860) of those in the reference group underwent a cervical exam (P<.01). Women exposed to the FightHPV app were 2 times more likely to attend screening (adjusted HR 2.3, 95% CI 2.0-2.7), during which they were 13 times more likely to be diagnosed with high-grade abnormality (adjusted HR 12.7, 95% CI 5.0-32.5) than the women in the reference group.

Conclusions
Exposure to the FightHPV app significantly increased cervical cancer screening attendance across the various analyses and improved detection of women with high risk for cervical cancer. For the first time, we demonstrated the effectiveness of gamification combined with mobile technology in cancer prevention by empowering women to make active health-related decisions. Gamification can significantly improve the understanding of complicated scientific concepts behind interventions and increase the acceptance of proposed cancer control measures.