bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self-adhesive hydrogel meshes reduce tissue incorporation and mechanical behavior versus microgrips self-fixation: a preclinical study
Authors: Benito-Martínez Selma , Rodríguez Marta , García-Moreno Francisca , Pérez-Köhler Bárbara , Peña Estefanía , Calvo Begoña , Pascual Gemma , Bellón Juan Manuel 
Published: January 2022

Abstract
Purpose
Atraumatic mesh fixation for abdominal hernia repair has been developed to avoid the disadvantages of classical fixation with sutures, which is considered a cause of chronic pain and discomfort. This study was designed to analyze, in the short and medium term, the biological and mechanical behavior of two self-fixing meshes compared to that of a polypropylene (PP) mesh fixed with a cyanoacrylate (CA) tissue adhesive.

Methods
Partial abdominal wall defects (6 × 4 cm) were created in New Zealand rabbits (n = 36) and repaired using a self-adhesive hydrogel mesh (Adhesix™), a self-gripping mesh (ProGrip™) or a PP mesh fixed with CA (Surgipro™ CA). After 14 and 90 days, the host tissue incorporation, macrophage response and biomechanical strength were examined.

Results
At 14 and 90 days, the ProGrip and Surgipro CA meshes showed good host tissue incorporation; however, the Adhesix implants presented poor integration, seroma formation and a higher degree of shrinkage. The Adhesix hydrogel was completely reabsorbed at 14 days, whereas ProGrip microhooks were observed at all study times. The macrophage response was higher in the ProGrip and Surgipro CA groups at 14 and 90 days, respectively, and decreased over time. At 90 days, the ProGrip implants showed the highest tensile strength values and the Adhesix implants showed the highest failure stretch.

Conclusion
Meshes with mechanical microgrip self-fixation (ProGrip) show better biological and mechanical behavior than those with adhesive hydrogel (Adhesix) in a preclinical model of abdominal hernia repair in rabbits.
Crosstalk between cancer-associated fibroblasts and immune cells in peritoneal metastasis: inhibition in the migration of M2 macrophages and mast cells by Tranilast
Authors: Nakamura Yusuke , Kinoshita Jun , Yamaguchi Takahisa , Aoki Tatsuya , Saito Hiroto , Hamabe-Horiike Toshihide , Harada Shinichi , Nomura Sachiyo , Inaki Noriyuki , Fushida Sachio 
Published: January 2022

Abstract
Background
The role of tumor–stroma interactions in tumor immune microenvironment (TME) is attracting attention. We have previously reported that cancer-associated fibroblasts (CAFs) contribute to the progression of peritoneal metastasis (PM) in gastric cancer (GC), and M2 macrophages and mast cells also contribute to TME of PM. To elucidate the role of CAFs in TME, we established an immunocompetent mouse PM model with fibrosis, which reflects clinical features of TME. However, the involvement of CAFs in the immunosuppressive microenvironment remains unclear. In this study, we investigated the efficacy of Tranilast at modifying this immune tolerance by suppressing CAFs.

Methods
The interaction between mouse myofibroblast cell line LmcMF and mouse GC cell line YTN16 on M2 macrophage migration was investigated, and the inhibitory effect of Tranilast was examined in vitro. Using C57BL/6J mouse PM model established using YTN16 with co-inoculation of LmcMF, TME of resected PM treated with or without Tranilast was analyzed by immunohistochemistry.

Results
The addition of YTN16 cell-conditioned medium to LmcMF cells enhanced CXCL12 expression and stimulated M2 macrophage migration, whereas Tranilast inhibited the migration ability of M2 macrophages by suppressing CXCL12 secretion from LmcMF. In PM model, Tranilast inhibited tumor growth and fibrosis, M2 macrophage, and mast cell infiltration and significantly promoted CD8 + lymphocyte infiltration into the tumor, leading to apoptosis of cancer cells by an immune response.

Conclusion
Tranilast improved the immunosuppressive microenvironment by inhibiting CAF function in a mouse PM model. Tranilast is thus a promising candidate for the treatment of PM.
Mathematical models of neuronal growth
Authors: Oliveri Hadrien , Goriely Alain 
Published: January 2022

AbstractThe establishment of a functioning neuronal network is a crucial step in neural development. During this process, neurons extend neurites—axons and dendrites—to meet other neurons and interconnect. Therefore, these neurites need to migrate, grow, branch and find the correct path to their target by processing sensory cues from their environment. These processes rely on many coupled biophysical effects including elasticity, viscosity, growth, active forces, chemical signaling, adhesion and cellular transport. Mathematical models offer a direct way to test hypotheses and understand the underlying mechanisms responsible for neuron development. Here, we critically review the main models of neurite growth and morphogenesis from a mathematical viewpoint. We present different models for growth, guidance and morphogenesis, with a particular emphasis on mechanics and mechanisms, and on simple mathematical models that can be partially treated analytically.
Expansion of Ash Dieback towards the scattered Fraxinus excelsior range of the Italian peninsula
Authors: Migliorini Duccio , Luchi Nicola , Nigrone Emanuele , Pecori Francesco , Pepori Alessia Lucia , Santini Alberto 
Published: January 2022

AbstractHymenoscyphus fraxineus, causal agent of Ash Dieback, has posed a threat to Fraxinus excelsior (common ash) in Europe since the 1990s. In south-western Europe, optimal climatic conditions for H. fraxineus become scattered and host density decreases, reducing disease spread rates. To date, the Ash Dieback agent has not been reported from southern and most of central Italy, where native F. excelsior is present as small fragmented populations. This study examines the expansion of Ash Dieback into central Italy, and it considers the consequences of further local spread with regards to the loss of F. excelsior genetic resource. Symptomatic F. excelsior were sampled from sixteen sites in northern and central Italy during 2020. Specimens were analyzed with a culturomics and a quantitative PCR approach. A bibliographic search of F. excelsior floristic reports was conducted for the creation of a detailed range map. The combined use of both techniques confirmed the presence of H. fraxineus in all the sites of central Italy where host plants were symptomatic. These new records represent the southern limit of the current known distribution of this pathogen in Italy, and together with Montenegro, in Europe. The characterization of the F. excelsior scattered range suggests that further spread of Ash Dieback across southern Italy is a realistic scenario. This presents a threat not just to the southern European proveniences of F. excelsior, but to the species as a whole, should Ash Dieback lead to the loss of warm climate adapted genetic material, which may become increasingly valuable under climate change.
Using Commercial Aircraft Meteorological Data to Assess the Heat Budget of the Convective Boundary Layer Over the Santiago Valley in Central Chile
Authors: Muñoz Ricardo C. , Whiteman C. David , Garreaud René D. , Rutllant José A. , Hidalgo Jacqueline 
Published: January 2022

AbstractThe World Meteorological Organization Aircraft Meteorological Data Relay (AMDAR) programme refers to meteorological data gathered by commercial aircraft and made available to weather services. It has become a major source of upper-air observations whose assimilation into global models has greatly improved their performance. Near busy airports, AMDAR data generate semi-continuous vertical profiles of temperature and winds, which have been utilized to produce climatologies of atmospheric-boundary-layer (ABL) heights and general characterizations of specific cases. We analyze 2017–2019 AMDAR data for Santiago airport, located in the centre of a $$40\times 100$$

40
×
100

km$$^2$$

2

subtropical semi-arid valley in central Chile, at the foothills of the Andes. Profiles derived from AMDAR data are characterized and validated against occasional radiosondes launched in the valley and compared with routine operational radiosondes and with reanalysis data. The cold-season climatology of AMDAR temperatures reveals a deep nocturnal inversion reaching up to 700 m above ground level (a.g.l.) and daytime warming extending up to 1000 m a.g.l. Convective-boundary-layer (CBL) heights are estimated based on AMDAR profiles and the daytime heat budget of the CBL is assessed. The CBL warming variability is well explained by the surface sensible heat flux estimated with sonic anemometer measurements at one site, provided advection of the cool coastal ABL existing to the west is included. However, the CBL warming accounts for just half of the mean daytime warming of the lower troposphere, suggesting that rather intense climatological diurnal subsidence affects the dynamics of the daytime valley ABL. Possible sources of this subsidence are discussed.
Spontaneous inversion of the submicron ceramic layer deposited on steel and the copper droplet positioned on their top (case of ceramic poorly wetted by liquid Cu)
Authors: Czagány M. , Koncz-Horváth D. , Baumli P. , Kaptay G. 
Published: January 2022

AbstractIn this paper, 50 … 680 nm thick AlN-Al2O3 coatings are deposited by magnetron sputtering on the surface of a steel substrate and a piece of copper is melted on top of the ceramic. Upon heating the ceramic layer is cracked, and the phase inversion of the two top phases from steel/ceramic/copper configuration to the steel/copper/ceramic configuration takes place within 30 s of liquid time of copper. This phase inversion process is accompanied by a Gibbs energy change of about − 1.78 J/m2, due to good wettability of solid deoxidized steel by liquid copper in contrary to poor wettability of the ceramic by the copper. When copper is melted on AlN-Al2O3 coating with its thicknesses smaller than a critical value of about 170 ± 60 nm, liquid copper droplets hanging down into the cracks within the ceramic reach the solid steel surface at the bottom of the cracks, thus the flow of Cu down along the cracks is enabled. However, when copper is melted on AlN-Al2O3 with its thickness larger than the critical value of 170 ± 60 nm, Cu first forms a non-wetting droplet on top of the ceramics, and only after a certain incubation time it starts flowing down the cracks. This incubation time was found to depend linearly on the thickness of the ceramic, as cracks are filled from the bottom upwards by liquid copper via the evaporation–condensation mechanism. By the end of the process, the steel/copper/ceramic configuration is further stabilized by gravity.
Graphical abstract
Endometriosis detection and localization in laparoscopic gynecology
Authors: Leibetseder Andreas , Schoeffmann Klaus , Keckstein Jörg , Keckstein Simon 
Published: January 2022

AbstractEndometriosis is a common gynecologic condition typically treated via laparoscopic surgery. Its visual versatility makes it hard to identify for non-specialized physicians and challenging to classify or localize via computer-aided analysis. In this work, we take a first step in the direction of localized endometriosis recognition in laparoscopic gynecology videos using region-based deep neural networks Faster R-CNN and Mask R-CNN. We in particular use and further develop publicly available data for transfer learning deep detection models according to distinctive visual lesion characteristics. Subsequently, we evaluate the performance impact of different data augmentation techniques, including selected geometrical and visual transformations, specular reflection removal as well as region tracking across video frames. Finally, particular attention is given to creating reasonable data segmentation for training, validation and testing. The best performing result surprisingly is achieved by randomly applying simple cropping combined with rotation, resulting in a mean average segmentation precision of 32.4% at 50-95% intersection over union overlap (64.2% for 50% overlap).
Allelic variation for alpha-Glucan Water Dikinase is associated with starch phosphate content in tetraploid potato
Authors: Uitdewilligen J. G. A. M. L. , Wolters A. M. A. , van Eck H. J. , Visser R. G. F. 
Published: January 2022

Abstract
Key message
Association analysis resulted in the identification of specific StGWD alleles causing either an increase or decrease in starch phosphate content which was verified in diploid and tetraploid potato mapping populations.

Abstract
Potatoes are grown for various purposes like French fries, table potatoes, crisps and for their starch. One of the most important aspects of potato starch is that it contains a high amount of phosphate ester groups which are considered to be important for providing improved functionalization after derivatization processes. Little is known about the variation in phosphate content as such in different potato varieties and thus we studied the genetic diversity for this trait. From other studies it was clear that the phosphate content is controlled by a quantitative trait locus (QTL) underlying the candidate gene α-Glucan Water Dikinase (StGWD) on chromosome 5. We performed direct amplicon sequencing of this gene by Sanger sequencing. Sequences of two StGWD amplicons from a global collection of 398 commercial cultivars and progenitor lines were used to identify 16 different haplotypes. By assigning tag SNPs to these haplotypes, each of the four alleles present in a cultivar could be deduced and linked to a phosphate content. A high value for intra-individual heterozygosity was observed (Ho = 0.765). The average number of different haplotypes per individual (Ai) was 3.1. Pedigree analysis confirmed that the haplotypes are identical-by-descent (IBD) and offered insight in the breeding history of elite potato germplasm. Haplotypes originating from introgression of wild potato accessions carrying resistance genes could be traced. Furthermore, association analysis resulted in the identification of specific StGWD alleles causing either an increase or decrease in starch phosphate content varying from 12 nmol PO4/mg starch to 38 nmol PO4/mg starch. These allele effects were verified in diploid and tetraploid mapping populations and offer possibilities to breed and select for this trait.
The role of novel instruments of brand communication and brand image in building consumers’ brand preference and intention to visit wineries
Authors: Gómez-Rico Mar , Molina-Collado Arturo , Santos-Vijande María Leticia , Molina-Collado María Victoria , Imhoff Brian 
Published: January 2022

AbstractThis research aims to analyze brand communication and brand image as specific drivers of wine brand preference and their influence on wine consumers’ intention to visit associated wineries. Specifically, this paper enhances the understanding of the roles of advertising-promotion, sponsorship-public relations, corporate social responsibility, and social media in brand communication, as well as functional, emotional and reputation components in brand image development in the context of wine tourism industry. Data was collected through a structured and self-administered questionnaire from 486 visitors to wineries in Spain. Partial least squares regression was used to evaluate the measurement model and the hypotheses. The empirical analysis shows that brand communication and brand image have similar positive effects on brand preference, and that brand image mediates the relationship between brand communication and brand preference. This research suggests implications for theory and practice relative to brand management in terms of communication and image; and it proposes insights into novel communication tools and marketing activities for the winery tourism industry. Firms should employ a holistic evaluation of brand communication to involve the whole organization, which would enhance the strategic role that brand communication plays.
Development of a tool to optimize economic and environmental feasibility of food waste chains
Authors: Celli Irene , Brunori Edoardo , Eugeni Michele , Cristinariu Cecilia Andrea , Zampilli Mauro , Massoli Sara , Bartocci Pietro , Caldarelli Valentina , Saetta Stefano , Bidini Gianni , Fantozzi Francesco 
Published: January 2022

AbstractThe Sustainable Development Goal 12.3 focuses on food and its inedible parts that exit the supply chain and thus are lost or wasted. This work addresses the food waste problem by presenting the development of a tool to design business models to reduce the production of food waste. This has been developed within the LIFE16 project iRexfo, coordinated by the University of Perugia. The tool aims at transferring the results obtained in a pilot region (Umbria, Italy) to 4 other regions in Europe. It has been coded in Python and has a graphical user interface (GUI) to insert inputs and display outputs. The GUI has been developed in FLASK and it is hosted in the website of PythonAnywhere. A case study on the application of the software is also presented, mainly based on data retrieved in the Umbria region, Italy. Together with economic analysis, also, environmental assessment is performed.
Anaerobic co-digestion of linen, sugar beet pulp, and wheat straw with cow manure: effects of mixing ratio and transient change of co-substrate
Authors: Elsayed Mahmoud , Andres Yves , Blel Walid 
Published: January 2022

AbstractThis study concerns the improvement and sustainability of producing methane (CH4) from the co-digestion of cow manure (CM), sugar beet pulp (SBP), linen (Ln), and wheat straw (WS). The first step involved co-digesting CM, Ln, and WS at various mixing ratios (CM/Ln/WS) in batch reactors to ascertain the best gas production. Biochemical methane potential (BMP) tests were carried out under mesophilic conditions using sludge from a wastewater treatment plant as an inoculum. The highest CH4 production (351 mL/g VSadd) and volatile solids removal rate (72.87%) were observed at the mixing ratio 50/25/25 and the lowest CH4 production (187 mL/g VSadd) was recorded at the ratio 25/25/50. A kinetic analysis was carried out to suggest the best strategy for methane production based on the ratio of substrates in the mix. The second step involved co-digesting CM, SBP, Ln, and WS in a semi-continuous stirred tank reactor to study the influence of a transient change in co-substrate on gas production and reactor performance. The rate of biogas production doubled with the transient change of co-substrate from WS to SBP, which may be due to the SBP being more easily biodegradable than WS.
Comparison of fatigue, cognitive dysfunction and psychological disorders in post-COVID patients and patients after sepsis: is there a specific constellation?
Authors: Stallmach Andreas , Kesselmeier Miriam , Bauer Michael , Gramlich Judith , Finke Kathrin , Fischer Anne , Fleischmann-Struzek Carolin , Heutelbeck Astrid , Katzer Katrin , Mutschke Stephanie , Pletz Mathias W. , Quickert Stefanie , Reinhart Konrad , Stallmach Zoe , Walter Martin , Scherag André , Reuken Philipp A. 
Published: January 2022

Abstract
Background
Sequelae of COVID-19 can be severe and longlasting. We compared frequencies of fatigue, depression and cognitive dysfunction in survivors of SARS-CoV-2-infection and sepsis.

Methods
We performed a prospective cohort study of 355 symptomatic post-COVID patients who visited our out-patient clinic for post-COVID-19 care. We compared them with 272 symptomatic patients from the Mid-German Sepsis Cohort, which investigates the long-term courses of sepsis survivors. Possible predictors for frequent clinical findings (fatigue, signs of depression, cognitive dysfunction) in post-COVID were investigated with multivariable logistic regression.

Results
Median age of the post-COVID patients was 51 years (range 17–86), 60.0% were female, and 31.8% required hospitalization during acute COVID-19. In the post-COVID patients (median follow-up time: 163 days) and the post-sepsis patients (180 days), fatigue was found in 93.2% and 67.8%, signs of depression were found in 81.3% and 10.9%, and cognitive dysfunction was found in 23.5% and 21.3%, respectively. In post-COVID, we did not observe an association between fatigue or depression and the severity of acute COVID-19. In contrast, cognitive dysfunction was associated with hospitalization (out-patient versus in-patient) and more frequent in post-COVID patients treated on an ICU compared to the MSC patients.

Conclusion
In post-COVID patients, fatigue and signs of depression are more common than in sepsis survivors, independent from the acute SARS-CoV-2-infection. In contrast, cognitive dysfunction is associated with hospitalization. Despite the differences in frequencies, owing to the similarity of post-COVID and post-sepsis sequelae, this knowledge may help in implementing follow-up approaches after SARS-CoV-2 infection.
Reveal Hydrogen Behavior at Grain Boundaries in Fe–22Mn–0.6C TWIP Steel via In Situ Micropillar Compression Test
Authors: Lu Xu , Wang Dong , Wan Di , Guo Xiaofei , Johnsen Roy 
Published: January 2022

AbstractIn this study, the effect of hydrogen on dislocation and twinning behavior along various grain boundaries in a high-manganese twinning-induced plasticity steel was investigated using an in situ micropillar compression test. The compressive stress in both elastic and plastic regimes was increased with the presence of hydrogen. Further investigation by transmission electron backscatter diffraction and scanning transmission electron microscope demonstrated that hydrogen promoted both dislocation multiplication and twin formation, which resulted in higher stress concentration at twin–twin and twin–grain boundary intersections.
Warum lebensweltlich deutschsprachige Schülerinnen und Schüler von einem sprachenintegrativen Deutschunterricht profitieren – empirische Erkenntnisse
Authors: Wildemann Anja , Bien-Miller Lena 
Published: January 2022

ZusammenfassungIn einem sprachenintegrativen Deutschunterricht werden die Kontaktsprachen der Schülerinnen und Schüler systematisch und zielorientiert einbezogen und für das sprachliche Lernen genutzt. Zur Wirksamkeit eines solchen sprachenintegrativen (Deutsch‑)Unterrichts auf die Reflexions- und Analysefähigkeiten lebensweltlich einsprachiger und mehrsprachiger Schülerinnen und Schüler liegen bislang keine Studien vor. Ein Zusammenhang zwischen der sprachenintegrativen und -vergleichenden Arbeit und der Entwicklung von Sprachbewusstheit ein- und mehrsprachiger Schülerinnen und Schüler wird zwar immer wieder vermutet, ist empirisch jedoch nicht belegt. Daran anknüpfend fokussiert der vorliegende Beitrag die Frage, ob sich ein sprachenintegrativer Deutschunterricht, in dem systematisch andere Sprachen vergleichend zur Zielsprache Deutsch thematisiert werden, positiv auf sprachbezogene Reflexions- und Analysefähigkeiten d. h. auf die Sprachbewusstheit, lebensweltlich ein- und mehrsprachiger Schülerinnen und Schüler auswirkt.