bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Ferroptosis-Related Genes and Pathways in Diabetic Kidney Disease Using Bioinformatics Analysis
Authors: Liu Dezhen , Zhou Wei , Mao Li , Cui Zhaohui , Jin Shanshan 
Published: September 2022

Abstract
Diabetic kidney disease (DKD) is a major public health issue because of its refractory nature. Ferroptosis is a newly coined programmed cell death characterized by the accumulation of lipid reactive oxygen species (ROS). However, the prognostic and diagnostic value of ferroptosis-related genes (FRGs) and their biological mechanisms in DKD remain elusive. The gene expression profiles GSE96804, GSE30566, GSE99339 and GSE30528 were obtained and analyzed. We constructed a reliable prognostic model for DKD consisting of eight FRGs (SKIL, RASA1, YTHDC2, SON, MRPL11, HSD17B14, DUSP1 and FOS). The receiver operating characteristic (ROC) curves showed that the ferroptosis-related model had predictive power with an area under the curve (AUC) of 0.818. Gene functional enrichment analysis showed significant differences between the DKD and normal groups, and ferroptosis played an important role in DKD. Consensus clustering analysis showed four different ferroptosis types, and the risk score of type four was significantly higher than that of other groups. Immune infiltration analysis indicated that the expression of macrophages M2 increased significantly, while that of neutrophils decreased significantly in the high-risk group. Our study identified and validated the molecular mechanisms of ferroptosis in DKD. FRGs could serve as credible diagnostic biomarkers and therapeutic targets for DKD.
Sleep to Reduce Incident Depression Effectively (STRIDE): Study protocol for a randomized controlled trial comparing stepped-care cognitive behavioral therapy for insomnia versus sleep education control to prevent major depression.
Authors: Drake Christopher L , Kalmbach David A , Cheng Philip , Ahmedani Brian K , Peterson Edward L , Joseph Christine LM , Roth Thomas , Kidwell Kelley M , Sagong Chaewon 
Published: September 2022

Abstract
Background
Prevention of major depressive disorder (MDD) is a public health priority. Strategies targeting individuals at elevated risk for MDD may guide effective preventive care. Insomnia is a reliable precursor to depression, preceding half of all incident and relapse cases. Thus, insomnia may serve as a useful entry point for preventing MDD. Cognitive-behavioral therapy for insomnia (CBT-I) is recommended as first-line treatment for insomnia, but widespread implementation is limited by a shortage of trained specialists. Innovative stepped-care approaches rooted in primary care can increase access to CBT-I and reduce rates of MDD.
Methods/Design:
We propose a large-scale stepped-care clinical trial in the primary care setting that utilizes a sequential, multiple assignment, randomized trial (SMART) design to determine the effectiveness of dCBT-I alone and in combination with clinician-led CBT-I for insomnia and the prevention of MDD incidence and relapse. Specifically, our care model uses digital CBT-I (dCBT-I) as a first-line intervention to increase care access and reduce need for specialist resources. Our proposal also adds clinician-led CBT-I for patients who do not remit with first-line intervention and need a more personalized approach from specialty care. We will evaluate negative repetitive thinking as a potential treatment mechanism by which dCBT-I and CBT-I benefit insomnia and depression outcomes.
Discussion
This project will test a highly scalable model of sleep care in a large primary care system to determine the potential for wide dissemination and implementation to address the high volume of population-need for safe and effective insomnia treatment and associated prevention of depression.
Trial Registration:
NCT03322774. Date of registration, October 26, 2017.
Impact of dissipative heat energy on the conducting Jeffery–Hamel KKL based nanofluid model: A numerical approach
Authors: Pattanaik P. Chandini , Mishra S. R. , Jena S. 
Published: September 2022

Abstract
The nanoparticles migration from the conventional buongirnio model is useful in several industrial applications as well as engineering and biomedical. Even if the blood flows through artery, the drug delivery process, etc. are more recent phenomena that are beneficial for the use of nanoparticles in the conventional liquid. Based upon the characteristics, the present study reveals the flow of conducting Jeffery-Hamel nanofluid for the inclusion of KKL (Koo- Kleinstreuer-Li) model conductivity within a stretching/shrinking channel surface as well as channel angle. Additionally, influence of dissipative for the interaction of both Joule and viscous and the radiative heat enrich the profiles significantly. The water-based nanoliquid is immersed with the CuO nanoparticle enhances the flow properties like thermal conductivity, viscosity, etc. Numerical treatment is adopted with the help of shooting based Runge-Kutta to carry forward the solutions for the flow profiles. The heat transfer rate as well as shear rate is deployed for the various parameters and analyzed their behaviors briefly.
Species C rotavirus infections in asymptomatic piglets in India, 2009-2013: Genotyping and phylogenetic analysis of all genomic segments
Authors: Joshi Madhuri S , Arya Shalu A. , Shinde Manohar S. , Ingle Vijay C. , Birade Hemant S. , Gopalkrishna Varanasi 
Published: September 2022

Abstract
Asymptomatic infection with Rotavirus C (RVC) at 20 percent detection rate was recorded in pigs from India. Sequencing of the VP6, VP7, and NSP4 genes of RVC strains identified I7/ I10, G1 and E5 genotypes, respectively. Full genome sequencing of one of these strains revealed additional P1, R1, C1, M3, A1, N5, T5, H1 genotypes, of VP4, VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP5 genes, respectively. Detection of porcine RVC strains at two different locations in India and at different time points strongly suggests their continuous circulation in the pig population through asymptomatic infections.
Tracking tree history to understand better natural dynamics of old-growth forest stands
Authors: Szegleti Zsófia , Vig Ákos , Ortmann-Ajkai Adrienne , Czóbel Szilárd , Horváth Ferenc 
Published: September 2022

Abstract
The long-term study of old-growth forest stands helps to understand the small-scale natural forest dynamic processes driven by the population biology of trees. The sampled trees can be considered from the viewpoint of their life and death cycle from establishment through to growing and death to full degradation. We provide a methodological framework for tracking and interpreting tree history in interaction with resurvey sampling events. The history of living and dead trees is consecutive and closely related to each other due to mortality events. The evanescent rate of disintegrated and decomposed dead trees is also a substantial dynamic characteristic of stands like regeneration, maturation, and mortality rates. They all form the intrinsic stand dynamic profile based on the whole life-dead-decomposed cycle of trees. This framework fills a knowledge gap in the European forest dynamic studies, where most of the research approaches point the focus on forest structure and pattern of developmental stages, and separately on living population dynamic features and deadwood structure and decay processes. In our pilot study, we performed resurvey of 53 Permanent Sampling Plots of a long untouched forest at Várhegy Strict Forest Reserve, Hungary. We estimated annual rates of ingrowth regeneration, maturation, mortality (13.2, 1.5, and 10.0 tree/hectare/year respectively), and 2.4 tree/hectare/year evanescence of disintegrated and decomposed dead trees. Apparent trends that accumulation of dead trees increases considerably – especially in oak-hornbeam stands, while 67% of annual regeneration ingrowth density is produced by shrubs, and only 9% by upper canopy trees.
In Search of the Hidden Protein: Optimization of Detection Strategies for autism-associated Activity-Dependent Neuroprotective Protein (ADNP) mutants.
Authors: D'Incal Claudio Peter , Cappuyns Elisa , Choukri Kaoutar , Szrama Kristy , De Man Kevin , Aa Nathalie Van der , Dijck Anke van , Kooy R. Frank , Berghe Wim Vanden 
Published: September 2022

AbstractHeterozygousde novomutations in theActivity-Dependent Neuroprotective Homeobox(ADNP) protein were found to be the common cause underlying the Helsmoortel-Van der Aa syndrome (HVDAS). With most of the patient mutations situated in the last exon, we previously demonstrated the predicted escape from nonsense-mediated decay by detecting mutantADNPmRNA. In this study wild-type and mutant forms of ADNP are investigated at the protein level and therefore optimal detection of the protein is required. We postulate that detection of ADNP by means of western blotting has been ambiguous and address different strategies to optimize the ADNP signal. Validation of a new N-terminal ADNP antibody (Aviva Systems) using a blocking peptide competition assay allowed to differentiate between specific and non-specific signals in different sample materials, resulting in a unique band signal around 150 kDa for ADNP, above its theoretical molecular weight of 124 kDa. Detection with different C-terminal antibodies confirmed the signals at an observed molecular weight of 150 kDa. By means of both a GFPSpark® and and Flag®-tag N-terminally fused to a human ADNP expression vector, we detected wild-type ADNP together with mutant forms after introduction of patient mutations inE. coliexpression systems by site-directed mutagenesis. However, western blot assessment of immortalized lymphoblastoid cell lines and post-mortem patient brain material failed to detect mutant ADNP protein, a scientific paradox up to today not yet resolved. This study aims to shape awareness for critical western blot assessment of ADNP variants and stimulates further research regarding ADNP expression by means of a validated multi-antibody approach.
Enhancement of Hydrogen Peroxide Production by RNA Polymerase rif Mutations of Lactobacillus delbrueckii subsp. lactis MDC 9617
Authors: Hovhannisyan Hrachya G. , Gaboyan Elen , Barseghyan Andranik H. , Pashayan Maria M. , Grigoryan Gohar G. , Danielyan Lusine V. 
Published: September 2022

Abstract

It was found out that among rifampicin resistant mutants (Rif) of Lactobacillus delbrueckii subsp. lactis MDC 9617 the frequency of occurrence of active hydrogen peroxide producers is about five times higher than in Rif + strain. Aerobically growing Rif mutants show relatively higher kinetics of H 2 O 2 production and earlier entry into the stationary phase due to auto-inhibition caused by inhibitory concentrations of H 2 O 2 . As a result, they synthesize about the same amount of H 2 O 2 but with a smaller biomass. The concentration of H 2 O 2 , which causes auto-inhibition of aerobically growing cultures is approximately 2 times lower than the MIC of exogenous H 2 O 2 for anaerobically growing cultures. The higher sensitivity of aerobically growing cultures is rather due to the existing intracellular H 2 O 2 , which together with extracellular H 2 O 2 causes an additive/cumulative effect. Lack of auto-inhibition at 5 °C allows the mutants to accumulate 50-70% more peroxide than the parental strain. Reduction of the food-borne pathogen Escherichia coli O157:H7 by selected active Rif-4 mutant during co-cultivation in LAPTg at 5 °C was significantly higher than that of the parental strain. Due to high (~40%) co-aggregation ability of LAB with E. coli , the death of the pathogen detected at an early stage of co-cultivation in the absence of inhibitory concentrations of H 2 O 2 , may occur by direct release of H 2 O 2 onto the target cell. The ability of Rif mutants to produce higher amounts of H 2 O 2 with a smaller biomass enable them to extend the shelf-life of refrigerated food without risk of altering sensory characteristics of the latter.
The Cameroon Health Research and Evidence Database (CAMHRED): tools, methods and application of a local evidence mapping initiative
Authors: Ongolo-Zogo Clémence , El-Khechen Hussein , Morfaw Frederick , Djiadjeu Pascal , Zani Babalwa , Darzi Andrea , Nji Paul Wankah , Nyambi Agatha , Youta Andrea , Zaman Faiyaz , Youmbi Cheikh Tchouambou , Siani Ines Ndzana , Mbuagbaw Lawrence 
Published: September 2022

Abstract
Background: Local evidence is important for contextualized knowledge translation. It can be used to adapt global recommendations, to identify future research priorities and inform local policy decisions. However, there are challenges in identifying local evidence in a systematic, comprehensive, and timely manner. There is limited guidance on how to map local evidence and provide it to users in an accessible and user-friendly way. In this study, we address these issues by describing the methods for the development of a centralized database of health research evidence for Cameroon and its applications for research prioritization and decision making. Methods: We searched 10 electronic health databases and hand-searched the archives of non-indexed African and Cameroonian journals. We screened titles, abstracts, and full texts of peer reviewed journal articles published between 1999 and 2019 in English or French that assess health related outcomes in Cameroonian populations. We extracted relevant study characteristics based on a pre-established guide. We developed a coding scheme or taxonomy of content areas so that local evidence is mapped to corresponding domains and subdomains. Pairs of reviewers coded articles independently and resolved discrepancies by consensus. Moreover, we developed guidance on how to search the database, use search results to create evidence maps and conduct knowledge gap analyses. Results: The Cameroon Health Research and Evidence Database (CAMHRED) is a bilingual centralized online portal of local evidence on health in Cameroon from 1999 onwards. It currently includes 4384 studies categorized into content domains and study characteristics (design, setting, year and language of publication). The database is searchable by keywords or through a guided search. Results including abstracts, relevant study characteristics and bibliographic information are available for users to download. Upon request, guidance on how to optimize search results for applications like evidence maps and knowledge gap analyses is also available. Conclusions: CAMHRED (https://camhred.org/) is a systematic, comprehensive, and centralized resource for local evidence about health in Cameroon. It is freely available to stakeholders and provides an additional resource to support their work at various levels in the research process.
Dispersal patterns and flight activity of Phyllophaga capillata (Blanchard) (Coleoptera: Melolonthidae) in soybeans in central Brazil
Authors: Rocha Marcus Vinícius Celani , Oliveira Charles Martins , Frizzas Marina Regina 
Published: September 2022

Abstract
Mark-release-recapture studies have been used to evaluate the dispersal ability of different insect species. In the field, we evaluated the dispersal ability of the soil pest species Phyllophaga capillata (Blanchard) (Coleoptera: Melolonthidae) in soybean (Glycine max L.) crop, and the flight activity of this species under controlled conditions. Field studies were conducted on a soybean seed production farm in Planaltina/DF, Brazil. On four dates, adults of P. capillata were collected, sexed, marked and released in a soybean plot (~ 230 ha). Twelve light traps were set in three concentric circles, with four traps each, at distances of 50, 150 and 250 m from the point of adult release. Under controlled conditions, groups of adults (males and females) were separated into pots containing soil, and the flight ability of the specimens was evaluated daily for 72 h. The average recapture rate of adults was 1.93% for the four collection dates. The highest recapture rate was observed at a distance of 50 m, at the beginning and middle of the swarming period, and at 250 m at the end of the swarm. Males, in general, were more active than females and reached the greatest distance of displacement (250 m). Most specimens (69.5% of males and 52.9% of females) were prone to leave the ground on the three consecutive days of the study. These results provide insights for understanding the dispersal patterns of this pest species in agricultural landscapes.
Using the city's cultural and historical assets to promote its Identity
Authors: Poyan Sayed Mustafa , Yushi Jiang 
Published: September 2022

Abstract
Propose:Today, cities compete to attract more tourists and visitors, and city branding plays a crucial part in this. It takes a long time for a city's identity to develop; it develops through time and becomes more culturally diverse as a consequence.Design/methodology/approachThis article focuses on the branding of Balkh province in northern Afghanistan by using its cultural and historical heritage assets. This article examines whether Balkh's unique identity may be used for city branding and analyzes which of the city's historical sites, important personalities, and historic events might be exploited as city brands. FindingResults from a questionnaire and SPSS 24 analysis of 111 people suggest that Balkh's strong identity, cultural and historical heritage assets may be used for its branding.Originality/valueThe other goal of this research is to find out how some of Balkh's historical and cultural assets could be used to create a city brand and show off the city's rich history.
Distribution of the Contemporary Precipitation Regime and the Impact of Climate Change on It Within the Territory of Azerbaijan
Authors: Huseynov J.S. Huseynov N.Sh. 
Published: September 2022

Abstract
The regularities of the contemporary distribution of the precipitation regime within the territory of Azerbaijan were researched in the research paper. The perennial tendency of the precipitation regime was determined using the rainfall observation data for the years between 1961 and 2020 in the conducted research. Based on the obtained results, a state-of-the-art precipitation map was compiled for the country. At the same time, the dynamics of the precipitation regime during the period 1961–2020, as opposed to the period 1881–1960, were evaluated. The research period was divided into two half-periods, namely 1961–1990 and 1991–2020, and the internal tendencies were analysed. The results of the investigation indicate that although the amount of the precipitation region underwent a downward trend across the country, the number of stations with a statistically significant decrease is small. Compared to the years 1881–1960, the amount of precipitation has decreased throughout the country. Moreover, the average annual temperature in the country increased by 0,80C from 1991 to 2020. This indicator is the same period between 1961 and 2020 compared to the period between 1881 and 1960. In order to conduct research, mathematical-statistical and cartographic methods were used.
The long non-coding RNA GhlncRNA149.1 improves cotton defense response to aphid damage as a positive regulator
Authors: Zhang jianmin , Zhang Yi , Hu Zongwei , Zhang Haoran , Zeng Man , Chen Qian , Wang Hainuo , An Hongliu , Yang Yazhen , Luo Xincheng , Xu Dong , Wan Peng 
Published: September 2022

Abstract
Long non-coding RNAs (lncRNAs) play crucial roles in plant various biological regulatory processes and plant defense response to all sorts of the biotic and abiotic stresses. However, studies on the functions and mechanisms of lncRNAs are still been scarely reported in plant defense response to the damage of phytophagous insects. Here, GhlncRNA149.1, a lncRNA from Gossypium hirsutum, was shown to be induced by Aphis gossypii, methyl jasmonate and salicylic acid. Overexpression of GhlncRNA149.1 in cotton plants improved their defense response to the damage of Aphis gossypii, while silence of GhlncRNA149.1 in cotton plants reduced their defense response to the damage of Aphis gossypii. The target gene GhA01G0129 of GhlncRNA149.1 was also induced by Aphis gossypii, methyl jasmonate, salicylic acid and it was highly expressed in cotton plants overexpressing GhlncRNA149.1, while lowly expressed in cotton plants silencing GhlncRNA149.1. The activity and expression of antioxidant enzymes superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) were up-regulated in transient overexpression GhlncRNA149.1 cotton plants, and their expression levels were down-regulated in GhlncRNA149.1 silenced cotton plants. In addition, the expression levels of Phenylalanine ammonialyase(PAL), Polyphenol oxidase(PPO), Enhanced disease susceptibility 1 (EDS1)and Nonexpressor of pathogenesis-related genes 1(NPR1) were up-regulated in cotton plants transiently overexpressing GhlncRNA149.1. These results collectively suggest that GhlncRNA149.1 improve the plant defense response to aphid attack, and thus has potential for enforing cotton aphid prevention and control.
Substitution of Concentrate Mixture with Dried Brewery Grains Improved biological and Economical Performance of 50% Dorper × Menz Crossbred Sheep
Authors: Mekonen Tefera , Alemayehu Liulseged , Eshete Mesfin , Kebede Ashenafi , Abebe Ayele , Bisrat Asfaw , Teferra Beneberu , Tsegahun Aschalew , Getachew Tesfa 
Published: September 2022

Abstract
The experiment was conducted to evaluate the potential of dried brewer's grains (DBG) to replace concentrate mixture (CM) in growing 50% Dorper × Menz crossbred ram lambs fed native pasture hay as basal diet. The experiment was arranged in a randomized complete block design (RCBD) with five treatments and seven replications, and initial body weight and age were used for blocking. Ram lamb from each block was randomly assigned to one of the five treatments. All sheep were fed native pasture hay (NPH) ad libtum at 20% refusal as a basal diet and supplemented with 400g CM (T1) or 300g DBG + 100 g CM (T2) or 200g DBG + 200g CM (T3) or 100g DBG + 300g CM (T4) or 400g DBG (T5). Natural pasture hay and total dry matter (TDM) intake were similar (P > 0.05) among the treatments, however, ME itntake was reduced (P < 0.0001) as CM was substituted with DBG. Substitution of 75, 50 and 100% of CM with DBG had greater (P < 0.001) body weight gain than 100% CM supplemented group, and substitution of 25% CM with DBG had an intermediate value. The partial budget analysis revealed that T3 and T5 gave greater net returns of Birr 881.19 and 854.91 per head, respectively. Based on MRR T3 is the best treatment and using this treatment a farmer can benefit ETB 8.94 for every 1 ETB investment. Hence, sole DBG or a mixture of DBG with a CM can be used for supplementation growing 50% Dorper crossbred ram lambs.
Immune-enhancing effect of heat-treated Levilactobacillus brevis KU15159 in RAW 264.7 cells
Authors: Hwang Chang-Hoon , Kim Kee-Tae , Lee Na‑Kyoung , Paik Hyun-Dong 
Published: September 2022

Abstract
Probiotics are alive microbes that present beneficial to the human’s health. They influence immune responses through stimulating antibody production, activating T cells, and altering cytokine expression. The probiotic characteristics of Levilactobacillus brevis KU15159 were evaluated on the tolerance and adherence to gastrointestinal conditions. L. brevis KU15159 was safe in a view of producing various useful enzymes and antibiotic sensitivity. Heat-treated L. brevis KU15159 increased production of nitric oxide (NO) and phagocytic activity in RAW 264.7 cells. In addition, heat-treated L. brevis KU15159 upregulated the expression of inducible nitric oxide synthase (iNOS) and proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, at protein as well as mRNA levels. In addition, the mitogen-activated protein kinase (MAPK) pathway, which regulates the immune system, was activated by heat-treated L. brevis KU15159. Therefore, L. brevis KU15159 exhibited an immune enhancing effect by the MAPK pathway in macrophage.