bluuebunny/crossref_metadata_2025_split huggingface dataset:
A miniaturized and integrated dual-channel fluorescence module for multiplex real-time PCR in the portable nucleic acid detection system
Authors: Fang Yile , Wang Yue , Su Xiangyi , Liu Haoran , Chen Hui , Chen Zhu , Jin Lian , He Nongyue 
Published: September 2022

A portable nucleic acid detection (PNAD) system based on real-time polymerase chain reaction (real-time PCR) has been developed for point-of-care testing (POCT) of infectious disease pathogens. In order to achieve “sample-in, result-out” while keeping the system compact, the hardware system integrates optical, thermal and motion control modules in a limited space for nucleic acid extraction, purification, amplification and detection. Among these hardware modules, the fluorescence module is one of the most important modules, because its performance directly affects the accuracy and sensitivity of the testing results. In this paper, a miniaturized, high-sensitivity and integrated dual-channel fluorescence module have been proposed for the homemade PNAD system. Based on the principle of confocal optical path, two group of excitation-emission optical paths of different wavelengths are integrated in a small space. In terms of circuitry, a current-light dual negative feedback light emitting diode (LED) drive circuit is applied to improve the stability of the excited light source. All optical and electronic components are integrated in a metal box of 55 mm × 45 mm × 15 mm, that helps miniaturize the detection system. Two different modules have been assembled to fit various fluorescent dyes or probes with the set of excitation and emission as follow: module 1#: 470 nm/525 nm, 570 nm/630 nm; module 2#: 520 nm/570 nm, 630 nm/690 nm. Finally, hepatitis B virus (HBV) concentration gradient detection and multiplex detection of different gene targets of SARS-CoV-2 are carried out on the PNAD system equipped with these two fluorescence modules for evaluating their performances. Compared with the commercial real-time PCR instrument, our fluorescence module has good stability and detection sensitivity.
Use and perception of information sources on COVID-19 measures by citizens of Belgium
Authors: van Loenhout Joris Adriaan Frank , Aujoulat Isabelle , Van den Broucke Stephan 
Published: September 2022

BackgroundThe effectiveness of adherence to COVID-19 infection prevention and control (IPC) measures depends on effective risk communication. This study assessed use and perception of COVID-19 information sources and channels in Belgium, and the relationship with adherence to measures.MethodsData were collected through an online questionnaire among a sample of 2008 respondents, representative for citizens of Belgium in terms of gender, age, socio-economic status, and region. Potential information sources named in the questionnaire were politicians, experts, journalists, and close contacts.ResultsOverall, experts contributed most to informing people on COVID-19 measures, and their information was considered clearest and most trustworthy, while politicians scored lowest for information clarity. All information channels were used by large proportions of respondents, namely television 80.2%, (online) newspapers 56.5%, radio 35.7% and social media 27.7%. Factors that contributed significantly to adherence in a multivariate model included use and perception of information from experts, which had a positive association, and relying on social media as an information channel, for which the relationship was negative.ConclusionsIt is important to achieve clear and trustworthy risk communication, as this contributes to overall adherence to IPC measures on COVID-19. Furthermore, attention should be given to the fact that for people who rely on social media as an information channel, their adherence can be adversely affected.
Three-dimensional displacement field of the 2010 Mw 8.8 Maule earthquake from GPS and InSAR data with the improved ESISTEM-VCE method
Authors: Xiong Luyun , Xu Caijun , Liu Yang , Zhao Yingwen , Geng Jianghui , Ortega-Culaciati Francisco 
Published: September 2022

The 2010 Mw 8.8 Maule earthquake occurred offshore central Chile and ruptured ∼500 km along the megathrust fault resulting from the oceanic Nazca plate subducting beneath the continental South American plate. The Maule earthquake produced remnant crustal displacements captured by a vast set of geodetic observations. However, given the nature of the observational techniques, it is challenging to extract its accurate three-dimensional coseismic deformation field with high spatial resolution. In this study, we modified the extended simultaneous and integrated strain tensor estimation from geodetic and satellite deformation measurements (ESISTEM) method with variance component estimation algorithm (ESISTEM-VCE) to retrieve the three-dimensional surface displacement field of this event by integrating the interferometric synthetic aperture radar (InSAR) and global positioning system (GPS) measurements. The ESISTEM-VCE method accounts for the spatial correlation of surface displacement among the adjacent points and determine the accurate weight ratios for different data sets, but also uses the uncertainties of GPS data and considers the different spatial scales from the different datasets. In the simulation experiments, the RMSEs of the ESISTEM-VCE method are smaller than those of the ESISTEM and ESISTEM-VCE (same d0) methods, and the improvements of 97.1%, 3.9%, and 84% are achieved in the east-west, north-south, and vertical components, respectively. Then, we apply the proposed methodology to the 2010 Mw 8.8 Maule earthquake, to obtain a three-dimensional displacement field that could provide fine deformation information. In the east-west component, the significant deformation is in the north of the epicenter, closed to the Constitución, with a maximum westward displacement of 495.5 cm. The displacement in the north-south component is relatively small compared to that in the east-west component. The maximum uplift reaches 211.8 cm, located at the southwest of the Concepción. Finally, the derived vertical displacements are also compared with field investigations, indicating that the ESISTEM-VCE method can obtain more accurate weight of different datasets and perform better than the ESISTEM method. The results highlight that the earthquake ruptured along the NE-SW direction, with a dominant thrust and a relatively small component of right-lateral strike-slip, coinciding with the characteristics of subduction and right-lateral shear. The experiments with the simulated and real data suggest that the improved ESISTEM-VCE method in this study is feasible and effective.
Paleoearthquakes of the Yangda-Yaxu fault across the Nujiang suture and Lancang river suture zone, southeastern Tibetan Plateau
Authors: Han Mingming , Chen Lichun , Li Yanbao , Gao Shuaipo , Feng Jiahui 
Published: September 2022

The WNW-trending Yangda-Yaxu fault (YYF) is located in the interior of the Qiangtang block (QTB). The YYF cuts through the Nujiang suture and Lancang river suture zone and divides Nujiang fault (NF) and Lancangjiang fault (LCJF) into two sections with significantly different activity levels, suggesting that the YYF may function as a specific structure in this region. In addition, a recent work argues that the YYF plays an important role in strain partitioning in southeastern Tibet and poses a high surface-faulting risk to the Sichuan-Tibet railway. However, no M ≥ 5.0 earthquakes have been recorded, and no palaeoseismic research has been conducted along the fault, leading to limited knowledge regarding its rupture behavior, which is essential for understanding regional tectonic deformation and assessing the regional seismic potential. In this study, we constrained the timings and recurrence intervals of late Quaternary paleoseismic events along the YYF for the first time. Through trench excavations and exposure cleaning combined with radiocarbon dating, five faulting events were identified, namely, E1 through E5 from youngest to oldest (831–1,220, 3,307–6,703, 9,361–10,286, 12,729–14,651, and before 14,651 yr BP). The recurrence interval of major earthquakes along the YYF follows a quasi-periodic pattern with an interval of ∼4,000 yr. Combining the clear linear geomorphic features along the fault and the paleoearthquake results in this paper, we believe that YYF is a newly-generated active fault, and has a significant control effect on the late Quaternary evolution of the NF and the LCJF. Further analysis revealed that the YYF also plays an important role in accommodating crustal deformation.
Reviewing the links and feedbacks between climate change and air pollution in Europe
Authors: Im Ulas , Geels Camilla , Hanninen Risto , Kukkonen Jaakko , Rao Shilpa , Ruuhela Reija , Sofiev Mikhail , Schaller Nathalie , Hodnebrog Øivind , Sillmann Jana , Schwingshackl Clemens , Christensen Jesper H. , Bojariu Roxana , Aunan Kristin 
Published: September 2022

Feedbacks between air pollutants and meteorology play a crucial role in the direction of the response of future climate and air pollution. These feedbacks are important to understand and quantify the potential impact of adaptation and mitigation policies setup for protecting the population against air pollution and heat stress. We review the interactions between climate and air pollution, with special focus on the projections of air pollution under different future climate scenarios and time horizons, based on a literature review of research articles and reports from the last decade. The assessment focuses on 1) the specific impacts of climate change on air pollution and natural particle and precursor emissions in Europe in the near future (2030), by mid-century (2050) and by end of the century (2100), 2) impacts on air pollution due to changes in emissions vs. changes in climate, 3) feedbacks from air pollution on climate, 4) impacts of climate change on wildland fires and air pollutant levels, and 5) the role of adaptation and mitigation policies on climate change and air pollution. Available literature to a large extent suggests that ozone concentrations will likely increase in the second half of the century by up to 9 ppb [−4 + 9.3], while in the first half of the century, changes are much smaller and are up to ±1.5 ppb. These changes are mainly attributed to increased temperatures and emissions of biogenic volatile organic compounds, but also depends on the models and scenarios used in these studies. On the other hand, the predicted changes in particle concentrations and chemical composition are uncertain and much smaller. Similar to ozone, larger changes in the particle concentrations are projected in the second half of the century. The main conclusion from this review is that the estimated changes in pollutant levels in the future vary significantly depending on the applied model systems, as well as the different emission or meteorological scenarios used in the different studies. Nevertheless, studies generally agree on the overall trend of the changes in pollutant levels due to climate change, in particular in the second half of the century.
Cellular, molecular and genomic alterations in the hatchlings of Labeo rohita after exposure to Triclosan
Authors: Sharma Sunil , Dar Owias Iqbal , Andotra Megha , Sharma Simran , Bhagat Ankeet , Thakur Sharad , Kesavan Anup Kumar , Kaur Arvinder 
Published: September 2022

Triclosan 5-chloro-2-(2, 4-dichlorophenoxy) phenol (TCS) is widely used as a biocide in human and veterinary medicines, personal care products and household articles. To obtain biomarkers for the acute stress of Triclosan, the hatchlings of Labeo rohita were exposed for 96 h to 0.06, 0.067 and 0.097 mg/L TCS. Morphological deformities, cell viability, frequency of micronucleated and aberrant cells, transcriptomic and biomolecular alterations were recorded after exposure and a depuration period of 10 days. The exposed hatchlings had a pointed head, curved trunk, lean body, deformed caudal fin, haemorrhage, hypopigmentation and tissue degeneration at 0.067 and 0.097 mg/L only. The frequency of viable cells declined but that of necrotic, apoptotic, micronucleated and abnormal cells increased (p ≤ 0.01) in a concentration dependent manner after exposure as well as the depuration period. After recovery, the frequency of viable and micronucleated cells increased, but that of necrotic, apoptotic, and aberrant cells declined in comparison to their respective 96 h values. The mRNA level of HSP47, HSP70, HSc71 and α-tropomyosin increased (p ≤ 0.01), while that of HSP60, HSP90, DHPR, myosin light polypeptide 3, desmin b and lamin b1 declined (p ≤ 0.01) after exposure. Ten days post exposure, a significant increase (p ≤ 0.01) over control was observed in the expression of all the heat shock and cytoskeletal genes and the values (except for HSc71) were higher than the respective 96 h values also. Infrared spectra showed that band area of amide A, amide I, amide II and phospholipids increased significantly (p ≤ 0.01) but peak intensity of lipid, glycogen and nucleic acids decreased after exposure. After recovery, area of the peaks for most of the biomolecules [except lipids (2924–2925, 1455–1457 cm−1) and glycogen (1163–1165 cm−1)] declined significantly over control and 96 h values. Collectively these changes seem to be responsible not only for the onset of paralysis but also for the concentration dependent increase in larval and cellular abnormalities as well as no/sporadic swimming movement in exposed hatchlings. It is evident that HSP60, HSc71, HSP90, α-tropomyosin and DHPR were strongly affected but DHPR can be used as the most sensitive marker for the toxicity of TCS. This is the first study reporting effect of TCS on the selected heat shock and cytoskeletal genes in a single model.
Anti-proliferative and pro-apoptotic effects of curcumin on skin cutaneous melanoma: Bioinformatics analysis and in vitro experimental studies
Authors: Li Long , Lu Shuwen , Ma Chao 
Published: September 2022

Objective: To reveal the potential mechanisms of curcumin for the treatment of skin cutaneous melanoma (SKCM) and its identify novel prognostic biomarkers.Methods: We searched the Cancer Genome Atlas and Traditional Chinese Medicine Systems Pharmacology database for the data on SKCM and curcumin. We conducted data analysis using R and online tools. The propagation and migration of SKCM cells were assessed with CCK-8 and scratch wound assays, respectively. We assessed apoptosis by TUNEL assay and western blot.Results: The survival analysis revealed that the mRNA expressions of DPYD, DPYS, LYN, PRKCQ, and TLR1 were significantly related to a favorable overall survival in SKCM patients. Additionally, the mRNA expression level of DPYD was associated with GPI, LYN, PCSK9, PRKCQ, and TLR1 mRNAs. GSEA results showed that the prognostic hub genes were augmented with ultraviolet, apoptosis, and metastasis. Curcumin expressed proliferation and migration of SK-MEL-1 cells (p &lt; 0.05), and induced apoptosis (p &lt; 0.05) significantly.Conclusion: Curcumin may have potential therapeutic effects in SKCM by inhibiting cell proliferation and migration and inducing apoptosis by regulating oxygen-related signaling pathways. The hub genes might be identified as novel biomarkers for SKCM.
Pancreatic family history does not predict disease progression but connotes alcohol consumption in adolescents and young adults with acute pancreatitis: Analysis of an international cohort of 2,335 patients
Authors: Juhász Márk Félix , Farkas Nelli , Szentesi Andrea , Wedrychowicz Andrzej , Nita Andreia Florina , Lásztity Natália , Tészás Alexandra , Tokodi István , Vincze Áron , Eross Bálint , Izbéki Ferenc , Czakó László , Papp Mária , Hegyi Péter , Párniczky Andrea 
Published: September 2022

BackgroundIn pediatric acute pancreatitis (AP), a family history of pancreatic diseases is prognostic for earlier onset of recurrent AP (ARP) and chronic pancreatitis (CP). No evidence supports the same association in adult-onset pancreatitis. Age-specific reasons for familial aggregation are also unclear. We aimed to examine the prognostic role of pancreatic family history for ARP/CP and observe possible underlying mechanisms.MethodsWe conducted a secondary analysis of the Hungarian Pancreatic Study Group’s (HPSG) multicenter, international, prospective registry of patients with AP, both children and adults. We compared the positive family history and the negative family history of pancreatic diseases, in different age groups, and analyzed trends of accompanying factors. Chi-square and Fisher exact tests were used.ResultsWe found a higher rate of ARP/CP in the positive pancreatic family history group (33.7 vs. 25.9%, p = 0.018), peaking at 6–17 years. Idiopathic AP peaked in childhood in the positive family history group (75% 0–5 years) and was consistently 20–35% in the negative group. A higher rate of alcohol consumption/smoking was found in the positive groups at 12–17 years (62.5 vs. 15.8%, p = 0.013) and 18–29 years (90.9 vs. 58.1%, p = 0.049). The prevalence of diabetes and hyperlipidemia steadily rose with age in both groups.ConclusionPositive family history most likely signifies genetic background in early childhood. During adolescence and early adulthood, alcohol consumption and smoking emerge—clinicians should be aware and turn to intervention in such cases. Contrary to current viewpoints, positive pancreatic family history is not a prognostic factor for ARP and CP in adults, so it should not be regarded that way.
Urine cytological study in patients with clinicopathologically confirmed neuronal intranuclear inclusion disease
Authors: Zhou Yiyi , Huang Pengcheng , Huang Zhaojun , Peng Yun , Zheng Yilei , Yu Yaqing , Zhu Min , Deng Jianwen , Wang Zhaoxia , Hong Daojun 
Published: September 2022

ObjectiveThe diagnosis of neuronal intranuclear inclusion disease (NIID) is currently based on CGG repeat expansion in the 5′UTR of the NOTCH2NLC gene, or p62-positive intranuclear inclusions in skin biopsy. The purpose of this study is to explore the value of non-invasive pathological findings in urine sediment cells from NIID patients.Materials and methodsTen patients with clinically suspected NIID were enrolled for skin biopsy and gene screening. Morning urine (500 ml) was collected from each patient, and cell sediment was obtained by centrifugation. Urine cytology, including Giemsa staining, p62 immunostaining, and electron microscopic examination, were conducted on cell sediment.ResultsThe main clinical symptoms of 10 patients included episodic disturbance of consciousness, cognitive impairment, tremor, limb weakness, and so on. Cerebral MRI showed that 9 patients had linear DWI high signal in the corticomedullary junction. Genetic testing found that the number of CGG repeat ranged from 96 to 158 in the NOTCH2NLC gene. Skin biopsy revealed that all patients showed p62-positive intranuclear inclusions in 18.5 ± 6.3% of the duct epithelial cells of sweat gland. In contrast, urine sediment smears revealed that only 3 patients had p62 positive intranuclear inclusions in 3.5 ± 1.2% of the sedimentary cells. Ultrastructural examinations showed that intranuclear inclusions were also identified in the cell sediment of the 3 patients.ConclusionUrine cytology may be a new and non-invasive pathological diagnosis technique for some NIID patients, although the positive rate is not as high as that of skin biopsy, which is a sensitive and reliable pathological method for NIID.
ERBB2 promoter demethylation and immune cell infiltration promote a poor prognosis for cancer patients
Authors: Wang Hongting , Jiang Yongxu , Jin Huanhuan , Wang Cunqin 
Published: September 2022

BackgroundReceptor tyrosine-protein kinase erbB-2 (ERBB2) expression is a critical factor for the prognosis of various cancers. ERBB2 enrichment indicates a poor prognosis in some cancer types but could be a favorable prognostic factor in others.MethodsWe analyzed DNA methylation, mRNA, protein, immune cell infiltration, and related signaling pathways using TIMER2.0, GEPIA2, STRING, and UALCAN portal datasets in tumor tissues of diverse cancer types and their matched normal tissues.ResultsERBB2 promoter demethylation increases transcript protein amplification and promotes a poor prognosis for cancer patients. ERBB2 gain-of-function procures immune cell infiltration for tumor growth and drives away T regulatory cells, which suppress or downregulate induction and proliferation of effector T cells. The downstream signaling pathways, such as tumor proliferation, ECM-related genes, and degradation of ECM, are involved in ERBB2 gene demethylation and immune activation in cancer progression.ConclusionERBB2 gene demethylation leads to a poor prognosis in cancer patients, which is strongly influenced by the composition and abundance of tumor immune cell infiltration. ERBB2 demethylation could be used in clinical practice to identify immune profiles and direct therapeutic strategies.
Urinary incontinence as a possible signal of neuromuscular toxicity during immune checkpoint inhibitor treatment: Case report and retrospective pharmacovigilance study
Authors: Hu Yizhang , Lu Wenchao , Tang Borui , Zhao Zhixia , An Zhuoling 
Published: September 2022

BackgroundImmune checkpoint inhibitors (ICIs) are associated with different immune-related adverse events (irAEs), but there is limited evidence regarding the association between urinary incontinence and ICIs.MethodsWe described the case of a patient experiencing urinary incontinence who later experienced a series of irAEs such as myocarditis, myositis, and neurologic diseases while on ICI treatment in our hospital. In addition, we queried the Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS) from the third quarter of 2010 to the third quarter of 2020 to perform a retrospective study to characterize the clinical features of urinary incontinence associated with ICIs.ResultIn the FAERS study, 59 cases of ICI-related urinary incontinence were retrieved, and approximately 32.2% of the cases were fatal. Combination therapy with nervous system drugs and age &gt;80 years old were the significant risk factors for fatal outcomes. Among these cases of ICI-related urinary incontinence, 40.7% (n = 24) occurred concomitantly with other adverse events, especially, neurological (fifteen cases), cardiovascular (seven cases), musculoskeletal (six cases), and urological disorders (five cases). Five cases had an overlapping syndrome similar to our case report, including one case of myasthenia gravis with myocarditis and another of myasthenic syndrome with polymyositis.ConclusionICI-related urinary incontinence might be a signal of fatal neuromuscular irAEs, especially when it occurs concomitantly with ICI-associated neuromuscular–cardiovascular syndrome. Clinicians should be aware of the occurrence of urinary incontinence to identify potentially lethal irAEs in the early phase.