bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of Monocyte Response by MicroRNA-15b/106a/374a During Antibody-mediated Rejection in Kidney Transplantation
Authors: Tinel Claire , Lamarthée Baptiste , Gazut Stéphane , Van Loon Elisabet , Von Tokarski Florent , Benon Aurélien , Sauvaget Virginia , Garcia-Paredes Victor , Ménager Mickaël , Morin Lise , Aouni Laïla , Cagnard Nicolas , Rabant Marion , Legendre Christophe , Terzi Fabiola , Essig Marie , Gwinner Wilfried , Naesens Maarten , Marquet Pierre , Anglicheau Dany 
Published: November 2022

Background.
Increasing evidence suggest that microRNAs are involved in the physiopathology of acute or chronic renal disease. In kidney transplantation, as key regulators of cellular homeostasis, microRNAs may be involved in the regulation of immune cell function and the allograft response. Here, we investigated the change in circulating microRNA expression profile and their involvement in the profound transcriptional changes associated with antibody-mediated rejection (AMR).

Methods.
Blood samples were collected at the time of the 710 kidney allograft biopsies at 4 European transplant centers. Messenger RNA and microRNA profiling analyses were performed in a discovery-to-validation study within 3 independent cohorts encompassing N = 126, N = 135, and N = 416 patients, respectively.

Results.
Compared with samples with no AMR, 14 microRNAs were significantly decreased in AMR samples. Among them, expression levels of microRNA-15b, microRNA-106a, and microRNA-374a gradually decreased with the severity of AMR lesions. From their in silico–predicted target genes, a high proportion proved to be significantly upregulated in the paired transcriptomic analysis. Gene ontology analyses of microRNA-15b/-106a/-374a suggested enrichment in myeloid-related pathways, which was further refined by in silico and ex vivo transcriptomic analyses, showing a specific origin from classical CD14+ monocytes. Finally, human CD14+ monocytes were subjected to transduction by antago-microRNAs to mimic AMR pathology. MicroRNA-15b/-106a/-374a impairment resulted in cellular activation with an increased expression of CD69, CRIM1, IPO7, and CAAP1, direct and common targets of the 3 microRNAs.

Conclusions.
Together, our data provide new insights into circulating microRNAs as markers and key players in AMR, and they suggest monocyte involvement in this process.
Impact of Recipient Age at Liver Transplant on Long-term Outcomes
Authors: Niazi Shehzad K. , Brennan Emily , Spaulding Aaron , Crook Julia , Borkar Shalmali , Keaveny Andrew , Vasquez Adriana , Gentry Melanie T. , Schneekloth Terry , Taner C. Burcin 
Published: November 2022

Background.
The age of a liver transplant (LT) candidate is one of many variables used in the transplant selection process. Most research about the age at transplant has used prespecified age ranges or categories in assessing associations with transplant outcomes. However, there is a lack of knowledge about the age at transplant and survival. This study aimed to examine associations of age at transplant as a continuous variable, in conjunction with other patient and disease-related factors, with patient and graft survival after LT.

Methods.
We used the Standard Transplant Analysis and Research data to identify LT recipients between January 2002 and June 2018. Cox regression models with a restricted cubic spline term for age examined associations with graft and patient survival after LT. We assessed the interactions of age with recipients’ sex, race/ethnicity, region, indication for transplant, body mass index, model for end-stage liver disease score, diabetes, functional status at transplant, and donor risk index.

Results.
Age at the time of LT showed a nonlinear association with both graft and patient survival. Each demographic, clinical, transplant-related, and donor-related factor influenced these relationships differently.

Conclusions.
Our results suggest that some older LT candidates may be better than some younger candidates and that clinicians should not exclusively use age to determine who receives LT.
Unrelated Stem Cell Donor HLA Match Likelihoods in the US Registry Incorporating HLA-DPB1 Permissive Mismatching
Authors: Gragert Loren , Spellman Stephen , Shaw Bronwen , Maiers Martin 
Published: November 2022

ABSTRACTDonor-recipient HLA matching at the DPB1 locus improves the outcomes of hematopoietic cell transplantation (HCT). Retrospective outcomes studies found that in transplants matched for all 8 alleles of the A, B, C, and DRB1 loci at high resolution (8-of-8 match), few transplants were also allele-matched at the DPB1 locus. DPB1 allele matching was thought to be logistically impractical, however a DPB1-permissive mismatch model based on T-cell epitope (TCE) reactivity expands the proportion of suitable donors. To understand the likelihood of success for finding a DPB1-permissive donor, we sought to expand population genetic match likelihood models for the US unrelated donor registry, National Marrow Donor Program (NMDP). After extending HLA haplotype frequency estimates to include the DPB1 locus, our models found that the likelihood of having a DPB1-permissive donor was not much lower than the 8-of-8 match likelihoods. A maximum of 5 additional donors would need to be typed to find a more optimal DPB1-permissive donor at least 90% of the time. Linkage disequilibrium patterns between the DPB1 locus and other classical HLA loci varied markedly by haplotype and population, indicating that the known recombination hotspot between DQ and DP gene complexes has not had uniform impact, thus DPB1-permissive donors are easier to identify within minority populations. DPB1 TCE categories were highly predictable from HLA typing at other loci when imputed with extended haplotype frequency data. Our overall results indicate that registry search strategies that seek a more optimally matched HCT donor encompassing HLA-DPB1 permissibility are likely to be highly productive.KEY POINTSHLA-DPB1 permissive donors are available for nearly all patients that have an 8/8 allele matchDPB1-permissive donors will be easier to identify among 8/8 allele matches for minority patients relative to White patients
The prevalence and factors associated with premature birth among post-delivery women in the Mbeya region: An analytical cross-sectional study
Authors: Mawolle Glory Godfrey , Moshi Fabiola Vincent 
Published: November 2022

AbstractIn Tanzania, there was an increase of prematurity rate from 11.4% in 2014 up to 16.60% in 2016 (1). This is a hospital based analytical cross-sectional study which involved biopsychosocial model, which focused on identifying prevalence and associated factors for preterm births among post-delivery women in Mbeya region, one of Tanzania regions. This study involved hospitals in Mbeya urban, Mbeya rural, Chunya, Kyela, Mbarali, Rungwe, Busokelo and Tukuyu districts, where the prevalence of preterm births in Mbeya found to be 39.1%. The study pointed out that factors associated with preterm births were child spacing of <24months (AOR=3.058; 95% CI = 1.026-9.116: p-value 0.045), non-effective use of malaria prophylaxis during pregnancy (AOR=5.418; 95% CI =1. p-value 0.008), twin pregnancy (AOR=4.657; 95% CI =2.112-10.223, p-value < 0.001), violence during pregnancy (AOR=2.059; p-value 0.048), lack of social support (AOR=1.993; p-value 0.022) and use of pica during pregnancy (AOR=1.880; p-value 0.029).The study outcome revealed that the prevalence of preterm births in Mbeya Region is even higher. Therefore, to minimize or eliminate the problem a deliberate effort to come up with strategies to improve family planning, applications of antimalaria prophylaxis, stop the use of pica and violence during pregnancy was highly recommended.
Topological network properties of resting-state functional connectivity patterns are associated with metal mixture exposure in adolescents
Authors: Invernizzi Azzurra , Rechtman Elza , Oluyemi Kristie , Renzetti Stefano , Curtin Paul , Colicino Elena , Ambrosi Claudia , Mascaro Lorella , Patrono Alessandra , Corbo Daniele , Cagna Giuseppa , Gasparotti Roberto , Reichenberg Abraham , Tang Cheuk Y. , Smith Donald R. , Placidi Donatella , Lucchini Roberto G. , Wright Robert O. , Horton Megan K. 
Published: November 2022

AbstractAdolescent exposure to neurotoxic metals adversely impacts cognitive, motor, and behavioral development. Few studies have addressed the underlying brain mechanisms of these metal-associated developmental outcomes. Furthermore, metal exposure occurs as a mixture, yet previous studies most often consider impacts of each metal individually. In this cross-sectional study, we investigated the relationship between exposure to neurotoxic metals and topological brain metrics (global and local efficiency) in adolescents. In 193 participants (53% females, ages: 15-25 years) enrolled in the Public Health Impact of Metals Exposure (PHIME) study, we measured concentrations of four metals (manganese, lead, copper and chromium) in multiple biological media (blood, urine, hair, and saliva) and acquired resting-state functional magnetic resonance imaging scans. Using graph theory metrics, we computed global and local efficiency (global:GE; local:LE) in 111 brain areas (Harvard Oxford Atlas). We used weighted quantile sum (WQS) regression models to examine association between metal mixtures and each graph metric (GE or LE), adjusted for sex and age. We observed significant negative associations between the metal mixture and GE and LE (βGE= -0.076, 95% CI [-0.122, -0.031]; βLE= -0.051, 95% CI [-0.095, -0.006]). Lead and chromium measured in blood contributed most to this association for GE, while chromium measured in hair contributed the most for LE. Our results suggest that exposure to this metal mixture during adolescence reduces the efficiency of integrating information in brain networks at both local and global levels, informing potential neural mechanisms underlying the developmental toxicity of metals. Results further suggest these associations are due to combined joint effects to different metals, rather than to a single metal.
A cost-effectiveness of Fecal DNA methylation test for colorectal cancer screening in Saudi Arabia
Authors: Yang Zhongzhou , Shi Mang , Liu Mengping , Wang Zhe , Huang Hui , Wang Shunyao , Zheng Xiaoyuan , Liu Yanyan , Liu Na , Li Yantao , Lau Eric , Zhu Shida 
Published: November 2022

AbstractBackgroundIn the Saudi Arabia, we estimated the cost-effectiveness between fecal DNA methylation test (FDMT) and fecal immunochemical testing (FIT) to detect colorectal cancer (CRC) and precancerous lesions in the national screening program.Participants and methodsA Markov model was used from 45 to 74 years old CRC screening to compare the cost-effectiveness with the FDMT vs FIT. We predicated the longitudinal participation patterns in the perfect adherence vs organized programs screening covered by national budgets. Outcomes incorporated the incidence rates and mortality rates, cost, quality-adjusted life-years (QALYs), incremental cost-effectiveness ratios (ICERs) under the perfect adherence as well as incidence and mortality forecast within 3, 6 and 9 years.ResultsUnder the perfect adherence, the total cost of FDMT was cheaper 38.16% than FIT and extends 0.22 QALYs per person. Furthermore, FDMT was more cost-effective as ICERs ($1487.30 vs $1982.42 per QALY saved) compared with FIT test. Therefore, FDMT test dominated than FIT every year (more costly and less effective). Compared with the organized FDMT programs (6.6% initial positive rate and 54% coloscopy compliance rate), the FIT program (5.8% initial positive rate and 48% coloscopy compliance rate) had 6.25 times to 7.76 times on the incidence rates; 5.12 times to 12.19 times on the mortality rates among 3, 6 and 9 years prediction.ConclusionsThrough the Markov model, we compared FDMT was less costly and more effective than the FIT test under the perfect and organized adherence within nine years prediction. It implied that FDMT might the novel cost-effective tool for Saudi Arabia national screening program.
The effectiveness of grass, birch, and mugwort pollen allergen immunotherapy over 18 years: A national cohort study in Denmark
Authors: Bager Peter , Poulsen Gry , Wohlfahrt Jan , Melbye Mads 
Published: November 2022

ABSTRACTBackgroundLong-term effectiveness of pollen allergen immune therapy (AIT) for allergic rhinitis (AR) has been studied for different durations. We studied effectiveness over 18 years using nationwide register-data for Denmark.MethodsA register-based cohort study using data on filled prescriptions, 1995-2018, Denmark. In a cohort of 1.1 million intranasal corticosteroid spray users (proxy for AR), we matched users treated with grass, birch or mugwort (GBM) AIT 1:2 with non-treated users on baseline year and 24 characteristics in the 3 years prior to baseline. The primary outcome was the odds ratio (OR) of using anti-allergic nasal spray during the pollen season in the treated vs. non-treated group modelled in a logistic generalized estimating equations analysis using an autoregressive correlation structure and adjusted for years since baseline (one year categories).ResultsAmong 7,914 AR patients treated with GBM AIT, the OR of using nasal spray 0-5 years after baseline was reduced when compared with 15,520 non-treated AR individuals (0-2 years, odds ratio (OR) 0.81(0.76-0.85); 3-5 years, OR 0.82(0.77-0.87)), but was close to unity or higher thereafter (6-9 years, OR 0.96(0.91-1.03); 10-18 years, OR 1.15(1.06-1.24). In post-hoc analyses, results were more consistent for those who already had 3 of 3 baseline years of use (0-2 years, OR 0.59 (0.54-0.64); 3-5 years, OR 0.64 (0.59-0.71); 6-9 years, 0.82 (0.75-0.91); 10-18 years, 0.95 (0.84-1.08), and in patients using nasal spray in the last pollen season (0-2 years, OR 0.76 (0.72-0.80); 3-5 years 0.84 (0.78-0.91); 6-9 years, OR 0.94 (0.86-1.03); 10-18 years, 0.90 (0.81-1.00)) as opposed to patients who did not use nasal spray in the last pollen season. The post-hoc findings were likewise more consistent for eye drop and oral antihistamine users (secondary outcomes).ConclusionPatients treated with GBM AIT in routine care to a higher degree stopped using anti-allergic nasal spray 0-5 years after starting the standard three years of therapy. Post-hoc analyses suggested results were more consistent among patients with persistent AR.
The State-Level Association Between Safe Haven Laws and Rates of Infant Mortality in the United States
Authors: Thomas Kathryn A. , Kaminsky Chloe J. 
Published: November 2022

AbstractContextAlthough the United States has safe haven laws, which allow mothers to safely relinquish their babies to designated safety points, rates of infant mortality remain significantly higher in the United States than other similarly developed countries. The current study is seeking to explore the state-level association between safe haven laws and infant mortality in the United States utilizing a legal epidemiological approach.MethodsSeveral sources of publicly available data were combined to examine the state-level association between safe haven laws and rates of infant mortality. A backward stepwise regression was used to determine whether certain safe haven laws significantly predicted rates of infant mortality, while controlling for demographic variables.FindingsAfter controlling for demographic variables including rates of health insurance and poverty, safe haven laws stating that only the mother can relinquish a child, laws that protect parents from criminal liability, and laws requiring the provider to provide legal information and referrals significantly predicted rates of infant mortality, with the total model accounting for 70.1% of variance in infant mortality.ConclusionsSeveral elements of safe haven laws significantly predicted higher rates of infant mortality, including laws stating that only the mother may relinquish the child, laws that do not protect parents from criminal liability, and laws requiring a provider to provide legal information and referrals. These results have important implications for policymakers considering the reform of the safe haven laws. It is especially important to evaluate the effectiveness and origins of safe haven laws in the wake of the overturn ofRoe v. Wade. Future studies should longitudinally examine how changes in safe haven laws lead to changes in rates of infant mortality.Policy Points⍰ This is the first study to use a legal epidemiological approach to examine the state-level impact of specific elements of safe haven laws on rates of infant mortality.⍰ Several elements of safe haven laws significantly predicted higher rates of infant mortality, including laws stating that only the mother may relinquish the child, laws that do not protect parents from criminal liability, and laws requiring a provider to provide legal information and referrals. These results have important implications for policymakers considering the reform of safe haven laws, which is especially important in the wake of the overturn ofRoe v. Wade.
Evaluation of the effectiveness of mass drug administration strategies for reducing scabies burden in Monrovia, Liberia: An agent-based modelling approach
Authors: Tellioglu Nefel , Chisholm Rebecca H. , Campbell Patricia Therese , Collinson Shelui , Timothy Joseph , Kollie Karsor , Zayzay Samuel , Devine Angela , McVernon Jodie , Marks Michael , Geard Nicholas 
Published: November 2022

AbstractBackgroundScabies is a parasitic infestation with high global burden. Mass drug administrations (MDAs) are recommended for communities with a scabies prevalence of >10%. Quantitative analyses are needed to demonstrate the likely effectiveness of MDA recommendations. In this study, we compare the effectiveness of differing MDA strategies, supported by improved treatment access, on scabies prevalence in Monrovia, Liberia.MethodsWe developed an agent-based model of scabies transmission calibrated to demographic and epidemiological data from Monrovia. We used this model to compare the effectiveness of MDA scenarios for achieving scabies elimination and reducing scabies burden, as measured by time until recrudescence following delivery of an MDA and disability-adjusted-life-years (DALYs) averted. We also investigated the additional impact of improving access to scabies treatment following delivery of an MDA.ResultsOur model showed that 3 rounds of MDA delivered at 6-month intervals and reaching 80% of the population could reduce prevalence below 2% for 3 years following the final round, before recrudescence. When MDAs were followed by increased treatment uptake, prevalence was maintained below 2% indefinitely. Increasing the number of and coverage of MDA rounds increased the probability of achieving elimination and the DALYs averted.ConclusionsOur results suggest that acute reduction of scabies prevalence by MDA can support a transition to improved treatment access. This study demonstrates how modelling can be used to estimate the expected impact of MDAs by projecting future epidemiological dynamics and health gains under alternative scenarios.SummaryWe use an agent-based model to demonstrate that mass drug administration (MDA) programs can achieve sustained reduction in scabies prevalence. However, effective MDAs must be accompanied by systemic changes that increase the rate of scabies treatment to prevent recrudescence.
Neonatal brain dynamic functional connectivity in term and preterm infants and its association with early childhood neurodevelopment
Authors: França Lucas G. S. , Ciarrusta Judit , Gale-Grant Oliver , Fenn-Moltu Sunniva , Fitzgibbon Sean , Chew Andrew , Falconer Shona , Dimitrova Ralica , Cordero-Grande Lucilio , Price Anthony N , Hughes Emer , O’Muircheartaigh Jonathan , Duff Eugene , Tuulari Jetro J , Deco Gustavo , Counsell Serena J , Hajnal Joseph V , Nosarti Chiara , Arichi Tomoki , Edwards A David , McAlonan Grainne , Batalle Dafnis 
Published: November 2022

AbstractBrain dynamic functional connectivity characterises transient connections between brain regions, changing over time. Features of brain dynamics have been linked to emotion and cognition in adult individuals, and atypical patterns have been associated with neurodevelopmental conditions such as autism. Although reliable functional brain networks have been consistently identified in neonates, little is known about the early development of dynamic functional connectivity. In this study we characterise dynamic functional connectivity with functional magnetic resonance imaging (fMRI) in the first few weeks of postnatal life in term-born (n= 324) and preterm-born (n= 66) individuals. We show that a dynamic landscape of brain connectivity is already established by the time of birth in the human brain, characterised by six transient states of neonatal functional connectivity with changing dynamics through the neonatal period. The pattern of dynamic connectivity is atypical in preterm-born infants, and associated with atypical social, sensory, and repetitive behaviours measured by the Quantitative Checklist for Autism in Toddlers (Q-CHAT) scores at 18 months of age.
Oral bioavailability and metabolism of hydroxytyrosol from food supplements
Authors: Bender Cecilia , Strassmann Sarah , Golz Christian 
Published: November 2022

AbstractTable olives and olive oils are the main dietary sources of hydroxytyrosol (HT), a natural antioxidant compound that has emerged as a potential aid in protection against cardiovascular risk. Bioavailability studies with olive oils showed that HT is bioavailable from its free form and from conjugated forms like oleuropein and its aglycone. Still, its low dietary intake, poor bioavailability, and high inter-individual variability after absorption through the gastrointestinal tract hamper its full benefits.In a randomized, controlled, blind, cross-over study, we investigated the impact of HT metabolism and bioavailability by comparing two olive-derived watery supplements containing different doses of HT (30.58 and 61.48 mg of HT/dosage, respectively); additionally, HT-fortified olive oil was used in the control group. To this aim, plasma and urine samples were evaluated in 12 healthy volunteers following the intake of a single dose of the supplements or fortified olive oil. Blood and urine samples were collected at baseline and at 0.5, 1, 1.5, 2, 4, and 12 hours after intake. HT and its metabolites were analyzed by UHPLC-DAD-MS/MS.Pharmacokinetic results showed that dietary HT administered through the food supplements is bioavailable and biovailability increases with the administered dose. After intake, homovanillic acid, HT-3-O-sulfate, and 3,4-dihydroxyphenylacetic acid are the main metabolites found both in plasma and urine. The maximum concentrations in plasma peaked 30 minutes after intake.Being the bioavailability of a compound a fundamental prerequisite for its effect, these results promise a good potential of both food supplements for the protection against oxidative stress and the consequent cardiovascular risk.