bluuebunny/crossref_metadata_2025_split huggingface dataset:
HNRNPH1 destabilizes the G-quadruplex structures formed by G-rich RNA sequences that regulate the alternative splicing of an oncogenic fusion transcript
Authors: Vo Tam , Brownmiller Tayvia , Hall Katherine , Jones Tamara L. , Choudhari Sulbha , Grammatikakis Ioannis , Ludwig Katelyn R. , Caplen Natasha J. 
Published: April 2022

ABSTRACTIn the presence of physiological monovalent cations, thousands of RNA G-rich sequences can form parallel G-quadruplexes (G4s) unless RNA-binding proteins inhibit, destabilize, or resolve the formation of such secondary RNA structures. Here, we have used a disease-relevant model system to investigate the biophysical properties of the RNA-binding protein HNRNPH1’s interaction with G-rich sequences. We demonstrate the importance of twoEWSR1-exon 8 G-rich regions in mediating the exclusion of this exon from the oncogenicEWS-FLI1transcripts expressed in a subset of Ewing sarcomas, using complementary analysis of tumor data, long-read sequencing, and minigene studies. We determined that HNRNPH1 binds theEWSR1-exon 8 G-rich sequences with low nM affinities irrespective of whether in a non-G4 or G4 state but exhibits different kinetics depending on RNA structure. Specifically, HNRNPH1 associates and dissociates from G4-folded RNA faster than the identical sequences in a non-G4 state. Importantly, we demonstrate using gel shift and spectroscopic assays that HNRNPH1, particularly the qRRM1-qRRM2 domains, destabilizes the G4s formed by theEWSR1-exon 8 G-rich sequences in a non-catalytic fashion. Our results indicate that HNRNPH1’s binding of G-rich sequences favors the accumulation of RNA in a non-G4 state and that this contributes to its regulation of RNA processing.
SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment during Bacillus subtilis sporulation
Authors: Winterhalter Charles , Stevens Daniel , Fenyk Stepan , Pelliciari Simone , Marchand Elie , Soultanas Panos , Ilangovan Aravindan , Murray Heath 
Published: April 2022

ABSTRACTBidirectional DNA replication from a chromosome origin requires the asymmetric loading of two helicases, one for each replisome. Our understanding of the molecular mechanisms underpinning helicase loading at bacterial chromosome origins is incomplete. Here we report both positive and negative mechanisms for directing helicase recruitment in the model organism Bacillus subtilis. Systematic characterization of the essential initiation protein DnaD revealed distinct protein interfaces required for homo-oligomerization, interaction with the master initiator protein DnaA, and interaction with the helicase co-loader protein DnaB. Informed by these properties of DnaD, we went on to find that the developmentally expressed repressor of DNA replication initiation, SirA, blocks the interaction between DnaD with DnaA, thereby inhibiting helicase recruitment to the origin during sporulation. These results advance our understanding of the mechanisms underpinning DNA replication initiation in B. subtilis, as well as guiding the search for essential cellular activities to target for antimicrobial drug design.
Transcriptional dynamics of transposable elements in the type I IFN response in Myotis lucifugus cells
Authors: Pasquesi Giulia Irene Maria , Kelly Conor J. , Ordonez Andrea D. , Chuong Edward B. 
Published: April 2022

ABSTRACTBackgroundBats are a major reservoir of zoonotic viruses, and there has been growing interest in characterizing bat-specific features of innate immunity and inflammation. Recent studies have revealed bat-specific adaptations affecting interferon (IFN) signaling and IFN- stimulated genes (ISGs), but we still have a limited understanding of the genetic mechanisms that have shaped the evolution of bat immunity. Here we investigated the transcriptional and epigenetic dynamics of transposable elements (TEs) during the type I IFN response in little brown bat (Myotis lucifugus) primary embryonic fibroblast cells, using RNA-seq and CUT&RUN.ResultsWe found multiple bat-specific TEs that undergo both locus-specific and family-level transcriptional induction in response to IFN. Our transcriptome reassembly identified multiple ISGs that have acquired novel exons from bat-specific TEs, including NLRC5, SLNF5 and a previously unannotated isoform of the IFITM2 gene. We also identified examples of TE-derived regulatory elements, but did not find strong evidence supporting genome-wide epigenetic activation of TEs in response to IFN.ConclusionCollectively, our study uncovers numerous TE-derived transcripts, proteins, and alternative isoforms that are induced by IFN in Myotis lucifugus cells, highlighting candidate loci that may contribute to bat-specific immune function.
Synaptotagmin 9 modulates spontaneous neurotransmitter release in striatal neurons by regulating substance P secretion
Authors: Seibert Michael J. , Evans Chantell S. , Stanley Kevin S. , Wu Zhenyong , Chapman Edwin R. 
Published: April 2022

ABSTRACTSynaptotagmin 9 (SYT9) is a tandem C2-domain Ca2+ sensor for exocytosis in neuroendocrine cells; its function in neurons remains unclear. Here, we show that endogenous SYT9 does not trigger rapid synaptic vesicle exocytosis in cultured cortical, hippocampal, or striatal neurons; rather, synaptotagmin 1 (SYT1) fulfills this function. SYT9 is able to regulate evoked synaptic vesicle exocytosis, but only when massively over-expressed. In striatal neurons, loss of SYT9 reduced the rate of spontaneous miniature neurotransmitter release events (minis). To delve into the underlying mechanism, we localized SYT9 within these neurons and found that it is targeted to dense core vesicles, where it regulates the release of substance P (SP), a neuropeptide known to modulate mini frequency. Exogenous SP, but not other striatal peptide hormones, completely rescued the Syt9 KO mini phenotype. Biochemical experiments revealed that Ca2+-binding to the C2A domain of SYT9 triggers membrane fusion in vitro, and mutations that disrupt this activity abolished the ability of SYT9 to regulate both SP release and mini frequency in striatal neurons. We conclude that SYT9 indirectly regulates synaptic transmission in striatal neurons by controlling SP release.
Face recognition under mask-wearing based on residual inception networks
Authors: Moungsouy Warot , Tawanbunjerd Thanawat , Liamsomboon Nutcha , Kusakunniran Worapan 
Published: April 2022

PurposeThis paper proposes a solution for recognizing human faces under mask-wearing. The lower part of human face is occluded and could not be used in the learning process of face recognition. So, the proposed solution is developed to recognize human faces on any available facial components which could be varied depending on wearing or not wearing a mask.Design/methodology/approachThe proposed solution is developed based on the FaceNet framework, aiming to modify the existing facial recognition model to improve the performance of both scenarios of mask-wearing and without mask-wearing. Then, simulated masked-face images are computed on top of the original face images, to be used in the learning process of face recognition. In addition, feature heatmaps are also drawn out to visualize majority of parts of facial images that are significant in recognizing faces under mask-wearing.FindingsThe proposed method is validated using several scenarios of experiments. The result shows an outstanding accuracy of 99.2% on a scenario of mask-wearing faces. The feature heatmaps also show that non-occluded components including eyes and nose become more significant for recognizing human faces, when compared with the lower part of human faces which could be occluded under masks.Originality/valueThe convolutional neural network based solution is tuned up for recognizing human faces under a scenario of mask-wearing. The simulated masks on original face images are augmented for training the face recognition model. The heatmaps are then computed to prove that features generated from the top half of face images are correctly chosen for the face recognition.
Measuring the impact of learning organization on proactive work behavior: mediating role of employee resilience
Authors: Malik Parul 
Published: April 2022

PurposeThe paper examines the role of learning organization in enhancing employees’ proactive work behavior. Furthermore, drawing on the conjectures of broaden-and-build and conservation of resources theory, this study tested the mediating role of employee resilience on the relationship between learning organization and proactive work behavior.Design/methodology/approachThe data for this study were collected over two measurement periods (six months apart) via a structured questionnaire among Indian IT/ITES (Information Technology Enabled Services) organizational employees. The study utilized confirmatory factor analysis to test the proposed measurement model. Additionally, the study employed Preacher and Hayes PROCESS macro to investigate the mediating effect of employee resilience.FindingsThe study results demonstrated that employee’s perceptions of learning organization (measured at time 1) positively predict proactive work behavior (measured at time 2), with the relationship being mediated by employee resilience (measured at time 1).Practical implicationsThe study asserts that organizational practitioners who aim to boost their employees’ proactive behavior need to first invest in nurturing learning organization and focus on building their employees’ resilient capability. As a matter of fact, despite focusing on alleviating the adverse events and chiefly emphasizing on stress management, the organizational practitioners should concentrate on building their employees’ resilient capability.Originality/valueThe present study explores the links between learning organization, employee resilience and proactive work behavior. This study tested a cohesive research model to investigate the role of both the contextual and individual resilient capability in enhancing employee proactivity at work. Furthermore, the study unfolds the underlying mechanism between the study variables by exploring the mediating role of employee resilience.
Growth and maintenance: how a subsidiary's environmental relationships with technological innovation affect its initiatives
Authors: Chen Lu-Jui , Tsou Hung-Tai , Lee Wen-Ruey 
Published: April 2022

PurposeIn this study, the authors argue that the host environment of subsidiaries may affect subsidiary initiative via relationships with two stakeholders: public and private stakeholders. On the one hand, the public relationships and private relationships of subsidiaries may affect their tendency to demonstrate initiative. On the other hand, including technological innovation as a moderating effect supports the tendency towards subsidiary initiative.Design/methodology/approachThis study obtained the data through a survey of 216 subsidiaries in China; chief executive officer or senior managers were selected as the data collection sources. AMOS analysis was used to address sophisticated data analysis issues.FindingsFindings based on samples from China support these arguments. The findings contribute to the literature by highlighting that different types of subsidiary initiative coexist within subsidiaries and by accounting for the external environmental relationships and technological innovation.Originality/valueWhat determines subsidiary initiatives in the host market? We find that (1) public relationships directly influence subsidiary initiatives, and (2) this effect is moderated by technological innovation. The theoretical framework shows that this interaction arises from the separate impacts of innovation characteristics, especially a foreign subsidiary's interest in entrepreneurial action affecting both growth and maintenance initiatives. In summary, this article concludes that initiatives are not simply the activities of subsidiaries. The authors hope that the strong explanatory and predictive power of these external factors and technological innovation are further enhanced when these concepts are integrated with the charters of internationalizing MNEs.
The impact of food culture on patronage intention of visitors: the mediating role of satisfaction
Authors: Khoshkam Mana , Marzuki Azizan , Nunkoo Robin , Pirmohammadzadeh Amin , Kiumarsi Shaian 
Published: April 2022

PurposeThe revival of local foods in the tourism context plays a significant role in attracting visitors. Unexpectedly, empirical evidence on food festivals aimed at reviving local cuisine in non-Western countries is scarce. This study aimed to examine and clarify the concept of food culture attributes on visitors' satisfaction and patronage intention in a food festival setting to revive local foods.Design/methodology/approachA quantitative approach was used. Convenience sampling and questionnaires were administered to 172 attendees as the final response to a food festival. The conceptual framework identifies the assumptions of structural equation modelling (SEM).FindingsFood culture attributes significantly impacted visitor satisfaction based on the results. Cooking methods had an insignificant influence on satisfaction and indirect effects on patronage intention. Additionally, the authors persuaded satisfaction to act as a mediator, and food culture was a significant contributor to the hypothesised framework.Originality/valueTo the best of the authors’ knowledge, this is one of the first empirical studies to employ food culture attributes (flavour, presentation, cooking method and gastronomic identification) and patronage intention in the mediating role of satisfaction with Iranian cultural heritage as a revival of a visitor’s attraction towards local cuisine. The results revealed that the resurrection of local dishes serves as an alternative truth that aids in preserving Iranian cultural heritage.
Professionalization and growth of a Kenyan family business – Jeff Hamilton
Authors: Muigai Sarah Watiri , Mungai Edward 
Published: April 2022

Learning outcomes
Upon completion of the analysis of the case, the students will be able to distinguish between a family business and a non-family business, evaluate the professionalization strategies used by Jeff Hamilton and categorize the type of family business that Jeff Hamilton is so far using the model of professionalization developed by Dekker et al. (2013). The model classifies family firms into four types according to their level of professionalization: autocracy, domestic configuration, administrative hybrid and a clench hybrid.

Case overview/synopsis
The case highlights how Jeff Hamilton, a family business that began in Kenya and has grown regionally in East Africa, has professionalized its operations and, by so doing, facilitated its growth. The family business is run by Major Boke and his wife Lucy Boke and was ranked number 31 in the 2019 top 100 SME survey conducted yearly by KPMG in collaboration with Nation media group – a Kenyan media company. The dilemma revolves around decision-making in the times of the COVID-19 pandemic, where structures put in place to professionalize the business facilitated the decision-making.

Complexity academic level
The case can be taught to undergraduate and graduate-level entrepreneurship and family business courses. It can also be taught to executive education short courses on family business and entrepreneurship.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 3: Entrepreneurship.
Thermal performance of an environmentally friendly nanoliquid in a cabinet with two l-shaped heaters: application for electronic cooling
Authors: Ma Yuan , Mohebbi Rasul , Yang Zhigang , Sheremet Mikhail 
Published: April 2022

Purpose
The purpose of this paper is to analyze numerically the nanofluid natural convection inside a square enclosure with two L-shaped heaters using lattice Boltzmann method.

Design/methodology/approach
An environmentally friendly nanofluid, clove-treated graphene nanoplatelet (CGNP), is used to study the enhancement of heat transfer. Six various heaters configurations are considered and effects of nanoparticle concentration (0–0.1%) and Rayleigh number (10^3–10^6) on streamlines, isothermal lines and heat transfer parameters are studied. The developed computational code has been validated using mesh sensitivity analysis and numerical data of other authors.

Findings
It is observed that in contrast to distilled water, CGNP/water nanofluid is an efficient coolant and the Nusselt number is increased as the nanoparticle concentration and Rayleigh numbers increment. The nanoparticle concentration cannot change the flow pattern inside the enclosure. However, the Rayleigh number and heaters configuration can change the flow pattern significantly. Several heaters configurations (Cases 1–4) related to the symmetry of geometrical shape and corresponding boundary conditions, illustrate the symmetry of streamlines and isotherms about the vertical line (X = 0.5). The formation of vortices inside the enclosure is affected by the raising heat plume above the heaters. Moreover, at different Rayleigh numbers, the relative magnitude of average Nu for various cases is different. At Ra = 103, the energy transport characteristic depends on the relative location of heaters and cold walls, and the order of average Nusselt number is Case 3 ˜ Case 4 ˜ Case 6 > Case 1 ˜ Case 2 ˜ Case 5. However, at Ra = 106, an influence of thermal convection mechanism on heat transfer is significant and the ranking of average Nusselt number is Case 1 ˜ Case 4 > Case 5 > Case 6 > Case 2 > Case 3.

Originality/value
The originality of the research lies in both the study of thermogravitational convection in a closed chamber with two L-shaped heaters, and the analysis of the influence of control parameters for an environmentally friendly nanoliquid on electronics cooling process.
Teacher mindset change in boundary-crossing lesson study:a case from China
Authors: Chen Xiangming , Ou Qunhui , An Chao , Zhang Dongyun 
Published: April 2022

PurposeThe purpose of this study is to provide an alternative approach to teacher learning on top of the usual practices of listening to experts' lectures and conducting school-based activities among peers in China. A boundary-crossing lesson study (BCLS) through school-university partnership served as an example to illustrate how a class teacher's mindset changed towards her students in equal interactions with university scholars.Design/methodology/approachWith the lenses of action science theory and boundary-crossing learning theory, the study used qualitative research approach to collect and analyze data. One Chinese primary school class teacher from a workshop on narrative action research was selected as the case for this study. Interviewing, observation and document analysis were used to collect data. Data analysis methods included categorization and contextualization of the teacher's mindset change towards her student.FindingsThe case teacher, Mrs. Li, collaborated closely in paired teaching with her university partner AP Yu in all the four phases of their BCLS. Each phase was marked with an interactive event such as dialogic illumination, reflexive theorization, embodied conversation, and fusion of teacher and trainer roles. With inspirational trust as a major interactive mechanism, Mrs. Li jumped out of her single-loop learning (changing strategies according to results) to double-loop learning (changing both strategies and values/values). As a result, her mindset changed from attribution to appreciation towards her low-achieving student.Originality/valueThis study made contributions in two ways. First, it examined a class teacher's mindset change towards her student, rather than that of subject matter teachers towards their teaching materials and methods. Second, it revealed how reflective interactions in a special kind of BCLS by school teachers and university scholars may promote the teacher's mindset change. The findings further confirm that having differences as boundaries is not enough for teacher learning. For deep learning like Mrs. Li's mindset change, it requires a respectful and inspirational relationship between school teachers and university scholars in the BCLS.
The role of culture as a determinant of tourism demand: evidence from European cities
Authors: Noonan Lisa 
Published: April 2022

Purpose
The purpose of this paper is to examine the impact of various cultural amenities on tourism demand in 168 European cities.

Design/methodology/approach
Using data from the European Commission’s Culture and Creative Cities Monitor 2017, a series of regressions are estimated to examine the impact of various cultural amenities on tourism demand while also controlling for other factors that may impact on tourism demand. Diagnostic tests are also conducted to check the robustness of the results.

Findings
The results reveal that cultural amenities in the form of sights, landmarks, museums, concerts and shows have a positive impact on tourism demand. By pinpointing the cultural amenities that are important for increasing tourism demand, the findings aid stakeholders in the tourism industry as they develop post-pandemic recovery plans.

Originality/value
This paper identifies two key aspects of the cultural tourism literature that require deeper investigation and aims to address these aspects. Firstly, while many studies focus on a specific or narrow range of cultural amenities, this study includes a series of measures to capture a range of cultural amenities. Secondly, while many studies are narrow in geographical scope, this paper includes data on 168 European cities across 30 countries.
A longitudinal analysis of country image and brand origin effects
Authors: Magnusson Peter , Zdravkovic Srdan , Westjohn Stanford A. 
Published: April 2022

PurposeThe objective of this study is to offer a longitudinal examination of country image, consumers’ brand origin recognition accuracy, and how their effects on brand evaluations have evolved over the last decade.Design/methodology/approachThe authors compare data gathered in 2020 against data gathered in 2008. Each data set consists of more than 500 American consumers evaluating approximately 12 different brands from three separate industries (i.e. auto, television, and fashion).FindingsThe findings indicate that country image can evolve over time and that its effect on brand evaluation persists even when inaccurate brand origin associations are made. The study offers meaningful insights for managers in understanding how brands’ country associations affect corresponding brand attitudes.Originality/valueSignificant research attention has been focused on the effects of country image. However, longitudinal analysis of the same research questions has been rare. This study offers a valuable comparison of how several important country-image related issues have evolved over time.