bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knockout of ABC transporter gene ABCA2 confers resistance to Bt toxin Cry2Ab in Helicoverpa zea
Authors: Fabrick Jeffrey A. , Heu Chan C. , LeRoy Dannialle M. , DeGain Ben A. , Yelich Alex J. , Unnithan Gopalan C. , Wu Yidong , Li Xianchun , Carrière Yves , Tabashnik Bruce E. 
Published: October 2022

AbstractEvolution of pest resistance reduces the benefits of widely cultivated genetically engineered crops that produce insecticidal proteins derived from Bacillus thuringiensis (Bt). Better understanding of the genetic basis of pest resistance to Bt crops is needed to monitor, manage, and counter resistance. Previous work shows that in several lepidopterans, resistance to Bt toxin Cry2Ab is associated with mutations in the gene encoding the ATP-binding cassette protein ABCA2. The results here show that mutations introduced by CRISPR/Cas9 gene editing in the Helicoverpa zea (corn earworm or bollworm) gene encoding ABCA2 (HzABCA2) can cause resistance to Cry2Ab. Disruptive mutations in HzABCA2 facilitated the creation of two Cry2Ab-resistant strains. A multiple concentration bioassay with one of these strains revealed it had > 200-fold resistance to Cry2Ab relative to its parental susceptible strain. All Cry2Ab-resistant individuals tested had disruptive mutations in HzABCA2. We identified five disruptive mutations in HzABCA2 gDNA. The most common mutation was a 4-bp deletion in the expected Cas9 guide RNA target site. The results here indicate that HzABCA2 is a leading candidate for monitoring Cry2Ab resistance in field populations of H. zea.
Scaling behavior of InAlN/GaN HEMTs on silicon for RF applications
Authors: Cui Peng , Zeng Yuping 
Published: October 2022

AbstractDue to the low cost and the scaling capability of Si substrate, InAlN/GaN high-electron-mobility transistors (HEMTs) on silicon substrate have attracted more and more attentions. In this paper, a high-performance 50-nm-gate-length InAlN/GaN HEMT on Si with a high on/off current (Ion/Ioff) ratio of 7.28 × 106, an average subthreshold swing (SS) of 72 mV/dec, a low drain-induced barrier lowing (DIBL) of 88 mV, an off-state three-terminal breakdown voltage (BVds) of 36 V, a current/power gain cutoff frequency (fT/fmax) of 140/215 GHz, and a Johnson’s figure-of-merit (JFOM) of 5.04 THz V is simultaneously demonstrated. The device extrinsic and intrinsic parameters are extracted using equivalent circuit model, which is verified by the good agreement between simulated and measuredS-parameter values. Then the scaling behavior of InAlN/GaN HEMTs on Si is predicted using the extracted extrinsic and intrinsic parameters of devices with different gate lengths (Lg). It presents that afT/fmaxof 230/327 GHz can be achieved whenLgscales down to 20 nm with the technology developed in the study, and an improvedfT/fmaxof 320/535 GHz can be achieved on a 20-nm-gate-length InAlN/GaN HEMT with regrown ohmic contact technology and 30% decreased parasitic capacitance. This study confirms the feasibility of further improvement of InAlN/GaN HEMTs on Si for RF applications.
The pulsating soft coral Xenia umbellata shows high resistance to warming when nitrate concentrations are low
Authors: Thobor Bianca , Tilstra Arjen , Bourne David G. , Springer Karin , Mezger Selma Deborah , Struck Ulrich , Bockelmann Franziska , Zimmermann Lisa , Yánez Suárez Ana Belén , Klinke Annabell , Wild Christian 
Published: October 2022

AbstractThe resistance of hard corals to warming can be negatively affected by nitrate eutrophication, but related knowledge for soft corals is scarce. We thus investigated the ecophysiological response of the pulsating soft coral Xenia umbellata to different levels of nitrate eutrophication (control = 0.6, medium = 6, high = 37 μM nitrate) in a laboratory experiment, with additional warming (27.7 to 32.8 °C) from days 17 to 37. High nitrate eutrophication enhanced cellular chlorophyll a content of Symbiodiniaceae by 168%, while it reduced gross photosynthesis by 56%. After additional warming, polyp pulsation rate was reduced by 100% in both nitrate eutrophication treatments, and additional polyp loss of 7% d−1 and total fragment mortality of 26% was observed in the high nitrate eutrophication treatment. Warming alone did not affect any of the investigated response parameters. These results suggest that X. umbellata exhibits resistance to warming, which may facilitate ecological dominance over some hard corals as ocean temperatures warm, though a clear negative physiological response occurs when combined with nitrate eutrophication. This study thus confirms the importance of investigating combinations of global and local factors to understand and manage changing coral reefs.
Research on visual question answering based on dynamic memory network model of multiple attention mechanisms
Authors: Miao Yalin , He Shuyun , Cheng WenFang , Li Guodong , Tong Meng 
Published: October 2022

AbstractSince the existing visual question answering model lacks long-term memory modules for answering complex questions, it is easy to cause the loss of effective information. In order to further improve the accuracy of the visual question answering model, this paper applies the multiple attention mechanism combining channel attention and spatial attention to memory networks for the first time and proposes a dynamic memory network model (DMN-MA) based on the multiple attention mechanism. The model uses the multiple attention mechanism in the situational memory module to obtain the most relevant visual vectors for answering questions based on continuous memory updating, storage and iterative inference of the questions, and effectively uses contextual information for answer inference. The experimental results show that the accuracy of the model in this paper reaches 64.57% and 67.18% on the large-scale public datasets COCO-QA and VQA2.0, respectively.
Resolving the paradox of unipolar induction: new experimental evidence on the influence of the test circuit
Authors: Baumgärtel Christof , Maher Simon 
Published: October 2022

AbstractA novel experiment has been devised shedding new light on the phenomenon of unipolar induction, also known as “Faraday’s Paradox”. This is a topic which continues to fascinate scientists and engineers with much debate continuing to this day. In particular, the question of the field co-rotating with the magnet or remaining stationary remains unsettled and supporting evidence exists for both positions. In this study, we present a novel experimental apparatus that includes, for the first time, the relative motion of the measurement circuit including the closing wires, as well as the magnet and disc respectively. The results show that the closing wire needs to be considered as part of the problem, which enables the apparent paradox associated with this phenomenon to be resolved. However, it remains impossible to tell if the field co-rotates with the magnet or if it remains stationary. Instead, direct electron interaction is considered as a viable alternative to resolve remaining paradoxes.
Integrated radiogenomics analyses allow for subtype classification and improved outcome prognosis of patients with locally advanced HNSCC
Authors: Rabasco Meneghetti Asier , Zwanenburg Alex , Linge Annett , Lohaus Fabian , Grosser Marianne , Baretton Gustavo B. , Kalinauskaite Goda , Tinhofer Ingeborg , Guberina Maja , Stuschke Martin , Balermpas Panagiotis , von der Grün Jens , Ganswindt Ute , Belka Claus , Peeken Jan C. , Combs Stephanie E. , Böke Simon , Zips Daniel , Troost Esther G. C. , Krause Mechthild , Baumann Michael , Löck Steffen 
Published: October 2022

AbstractPatients with locally advanced head and neck squamous cell carcinoma (HNSCC) may benefit from personalised treatment, requiring biomarkers that characterize the tumour and predict treatment response. We integrate pre-treatment CT radiomics and whole-transcriptome data from a multicentre retrospective cohort of 206 patients with locally advanced HNSCC treated with primary radiochemotherapy to classify tumour molecular subtypes based on radiomics, develop surrogate radiomics signatures for gene-based signatures related to different biological tumour characteristics and evaluate the potential of combining radiomics features with full-transcriptome data for the prediction of loco-regional control (LRC). Using end-to-end machine-learning, we developed and validated a model to classify tumours of the atypical subtype (AUC [95% confidence interval] 0.69 [0.53–0.83]) based on CT imaging, observed that CT-based radiomics models have limited value as surrogates for six selected gene signatures (AUC < 0.60), and showed that combining a radiomics signature with a transcriptomics signature consisting of two metagenes representing the hedgehog pathway and E2F transcriptional targets improves the prognostic value for LRC compared to both individual sources (validation C-index [95% confidence interval], combined: 0.63 [0.55–0.73] vs radiomics: 0.60 [0.50–0.71] and transcriptomics: 0.59 [0.49–0.69]). These results underline the potential of multi-omics analyses to generate reliable biomarkers for future application in personalized oncology.
Expanded endoscopic endonasal transsphenoidal approach to determine morphological characteristics and clinical considerations of the cavernous sinus venous spaces
Authors: Zhan Guowen , Guo Shanshan , Hu Honglei , Liao Jianchun , Dang Ruishan , Yang Youxiong 
Published: October 2022

AbstractThe study aimed at investigating the morphological characteristics and interconnected regularities of the cavernous sinus (CS) venous spaces using an expanded endoscopic endonasal transsphenoidal approach. Surgical dissections were performed for 15-colored silicon-injected human head specimens. The CS venous spaces were examined for their morphological and clinical characteristics using an expanded endoscopic endonasal transsphenoidal approach. The intracavernous course of the internal carotid artery (ICA) divided the CS venous spaces into four interconnected virtual compartments: medial, anteroinferior, posterosuperior, and lateral. The CS venous spaces had peculiar morphological characteristics; the medial compartment was C-shaped while the anteroinferior compartment resembled a boat's bow. The mean distances from the medial border of the inferior horizontal segment of cavernous ICA to the mid-line of the pituitary gland (PG) were 6.07 ± 1.61 mm (left) and 5.97 ± 1.89 mm (right); the mean distances from the medial border of the subarachnoid segment of cavernous ICA to the mid-line of the PG were 5.77 ± 1.16 mm (left) and 5.63 ± 1.17 mm (right); the mean distances from the medial border of the anterior vertical segment of cavernous ICA to the mid-line of the PG were 10.27 ± 1.74 mm (left) and 10.47 ± 1.90 mm (right). Morphological characteristics and the knowledge of the interconnected regularities of the CS venous spaces may help surgeons accurately locate the neurovascular structure, and thus may contribute to the effective prediction of tumor invasion and extension during endoscopic CS surgery.
Association between albumin or prealbumin levels at different stages and prognosis in severe acute pancreatitis: a 5-year retrospective study
Authors: Ni Tongtian , Wen Yi , Wang Yihui , Jiang Weisong , Sheng Huiqiu , Chen Erzhen , Mao Enqiang , Lan Zhixin , Huang Yaguo , Zhou Yuhua 
Published: October 2022

AbstractSevere acute pancreatitis (SAP) is a common abdominal disorder contributing to high mortality and open laparotomy rates. The role of exogenous infused albumin in fluid resuscitation or continuous therapy has always been an unanswered question. In early stage after onset, SAP patients with higher serum albumin or prealbumin show a better prognosis. In this study, we tried to disclose the linkage between albumin/prealbumin and SAP prognosis and establish a new goal-directed therapy involving albumin and prealbumin. Pearson’s chi-squared test and the Mann–Whitney U test were used to compare the descriptive data between surviving and non-surviving patients. Three days, 4–7 days, 8–14 days and 15–28 days after SAP onset were defined as stages 1–4. The average concentrations of albumin and prealbumin were calculated, and receiver operating characteristic (ROC) curves were drawn to screen out the best cutoff values associated with poor prognostic outcomes, including laparotomy and failure to survive. Kaplan–Meier survival curves and log-rank tests were used to validate the effect of the cut-off values. A total of 199 admitted patients were enrolled in this study. According to the analysis of the ROC curve, the serum albumin value should be raised to 35, 37, 40 and 42 g/L in the 1–4 stage after onset. Serum prealbumin values should be raised to 108, 180, and 181 g/L in stages 2–4 after onset. The validity of the above data was confirmed by Kaplan–Meier survival curves. Serum albumin and prealbumin levels in the early stage of SAP are significantly relevant to prognosis. Albumin should be infused from the fluid resuscitation stage to continuous therapy in order to reach the targets mentioned above. The increase in prealbumin depends on the early initiation of enteral nutrition and this also helps to ameliorate the prognosis.
Altered functional responses by PAR1 agonist in murine dextran sodium sulphate-treated colon
Authors: Sung Tae Sik , Moon Suk Bae , Perrino Brian A. , Sanders Kenton M. , Koh Sang Don 
Published: October 2022

AbstractProtease-activated receptor-1 (PAR1) is highly expressed in murine colonic smooth muscles. Responses to PAR1 activation are complex and result from responses in multiple cell types. We investigated whether PAR1 responses are altered in inflamed colon induced by dextran sodium sulfate (DSS)-treatment. Colitis was induced in C57BL/6 mice by administration of 3% DSS in drinking water for 7 days. Measurements of isometric force, transmembrane potentials from impaled smooth muscle cells, quantitative PCR and Western blots were performed. Thrombin, an activator of PAR1, caused transient hyperpolarization and relaxation of untreated colons, but these responses decreased in DSS-treated colons. Apamin caused depolarization and increased contractions of muscles from untreated mice. This response was decreased in DSS-treated colons. Expression of Kcnn3 and Pdgfra also decreased in DSS-treated muscles. A second phase of thrombin responses is depolarization and increased contractions in untreated muscles. However, thrombin did cause depolarization in DSS-treated colon, yet it increased colonic contractions. The latter effect was associated with enhanced expression of MYPT1 and CPI-17. The propagation velocity and frequency of colonic migrating motor complexes in DSS-treated colon was significantly higher compared to control colons. In summary, DSS treatment causes loss of transient relaxations due to downregulation of SK3 channels in PDGFRα+ cells and may increase contractile responses due to increased Ca2+ sensitization of smooth muscle cells via PAR1 activation.
MatchMiner: an open-source platform for cancer precision medicine
Authors: Klein Harry , Mazor Tali , Siegel Ethan , Trukhanov Pavel , Ovalle Andrea , Vecchio Fitz Catherine Del , Zwiesler Zachary , Kumari Priti , Van Der Veen Bernd , Marriott Eric , Hansel Jason , Yu Joyce , Albayrak Adem , Barry Susan , Keller Rachel B. , MacConaill Laura E. , Lindeman Neal , Johnson Bruce E. , Rollins Barrett J. , Do Khanh T. , Beardslee Brian , Shapiro Geoffrey , Hector-Barry Suzanne , Methot John , Sholl Lynette , Lindsay James , Hassett Michael J. , Cerami Ethan 
Published: October 2022

AbstractWidespread, comprehensive sequencing of patient tumors has facilitated the usage of precision medicine (PM) drugs to target specific genomic alterations. Therapeutic clinical trials are necessary to test new PM drugs to advance precision medicine, however, the abundance of patient sequencing data coupled with complex clinical trial eligibility has made it challenging to match patients to PM trials. To facilitate enrollment onto PM trials, we developed MatchMiner, an open-source platform to computationally match genomically profiled cancer patients to PM trials. Here, we describe MatchMiner’s capabilities, outline its deployment at Dana-Farber Cancer Institute (DFCI), and characterize its impact on PM trial enrollment. MatchMiner’s primary goals are to facilitate PM trial options for all patients and accelerate trial enrollment onto PM trials. MatchMiner can help clinicians find trial options for an individual patient or provide trial teams with candidate patients matching their trial’s eligibility criteria. From March 2016 through March 2021, we curated 354 PM trials containing a broad range of genomic and clinical eligibility criteria and MatchMiner facilitated 166 trial consents (MatchMiner consents, MMC) for 159 patients. To quantify MatchMiner’s impact on trial consent, we measured time from genomic sequencing report date to trial consent date for the 166 MMC compared to trial consents not facilitated by MatchMiner (non-MMC). We found MMC consented to trials 55 days (22%) earlier than non-MMC. MatchMiner has enabled our clinicians to match patients to PM trials and accelerated the trial enrollment process.
Fat infiltration in the infarcted heart as a paradigm for ventricular arrhythmias
Authors: Sung Eric , Prakosa Adityo , Zhou Shijie , Berger Ronald D. , Chrispin Jonathan , Nazarian Saman , Trayanova Natalia A. 
Published: October 2022

AbstractInfiltrating adipose tissue (inFAT) has been recently found to co-localize with scar in infarcted hearts and may contribute to ventricular arrhythmias (VAs), a life-threatening heart rhythm disorder. However, the contribution of inFAT to VA has not been well-established. We investigated the role of inFAT versus scar in VA through a combined prospective clinical and mechanistic computational study. Using personalized computational heart models and comparing the results from simulations of VA dynamics with measured electrophysiological abnormalities during the clinical procedure, we demonstrate that inFAT, rather than scar, is a primary driver of arrhythmogenic propensity and is frequently present in critical regions of the VA circuit. We determined that, within the VA circuitry, inFAT, as opposed to scar, is primarily responsible for conduction slowing in critical sites, mechanistically promoting VA. Our findings implicate inFAT as a dominant player in infarct-related VA, challenging existing paradigms and opening the door for unexplored anti-arrhythmic strategies.
Attentional Demand of Motor Speech Encoding: Evidence From Parkinson's Disease
Authors: Fournet Maryll , Catalano Chiuvé Sabina , Laganaro Marina 
Published: October 2022

Purpose:
While the involvement of attention in utterance planning is well established at the conceptual and lexical levels, the attentional demands of postlexical processes are still debated. This study investigates the involvement of attentional resources on motor speech encoding during utterance production in the context of Parkinson's disease (PD), a population allowing to assess if the attentional demands observed in a dual-task paradigm (the dual-task costs [DTCs]) are explained by postlexical difficulties and not solely by executive impairment.

Method:
Speech production was analyzed in a dual-task paradigm with 30 participants presenting with motor speech disorders due to hypokinetic dysarthria in the context of PD. The dual-task comprised an automatic speech task in which participants recited the days of the week and two nonverbal tasks evaluating processing speed and inhibition. The severity of dysarthria and performance in several executive tests (inhibition, verbal fluency, and cognitive shifting) were used as potential predictors of the DTCs.

Results:
Individuals with PD exhibited a DTC on the nonverbal tasks and on the speech task when the secondary task was inhibition (the most difficult one). Additionally, the severity of dysarthria and a poorer performance in cognitive shifting predicted a more severe DTC on speech rate. Finally, modulation of the magnitude of the DTCs was observed, depending on the difficulty of the nonverbal secondary task.

Conclusion:
The results suggest that, in PD, postlexical processes require attentional resources and cognitive shifting is related to dual-task performance in speech.

Supplemental Material:

https://doi.org/10.23641/asha.21265893
Reflecting on the Role of Gender and Race in Speech-Language Pathology
Authors: Holt Yolanda 
Published: October 2022

Purpose:The presented work was invited following the American Speech-Language-Hearing Association SIG 19 Virtual Talk “Speech Science in Diverse Populations” that occurred on September 2, 2021. The purpose of this article was to introduce the historical and theoretical frameworks of gender and race from a decidedly North American (United States) perspective to an audience that may be less familiar with those topics as they relate to the practice of communication sciences and disorders. Race and gender are huge topics. Entire fields of study and lifetimes of work are dedicated to understanding these constructs. Therefore, it is hoped that this brief review of race and gender will prompt the reader to evaluate how the two constructs are used to categorize people and whether being a member of a marginalized or a minoritized group affects the person's access to or use of intervention services. A critical theoretical discussion of race and gender is beyond the scope of this text. In this limited space, this work presents an overview of current and historical discussions of gender and race and a challenge to the reader to accept that their perspective is indebted to a specific belief system. In the United States, that belief system often evaluates human differences into binary categories on a weighted continuum. Speech-language professionals often use that continuum to identify and measure difference into either acceptable variation or disorder.Conclusions:The profession of speech-language pathology was established during a time when variation from middle-class White American communication norms was frequently defined as undesirable and sometimes as disordered. The communities and individuals we encounter deserve to be accepted as they are. We must resolve to expect and accept wide variation in human communication without pathologizing its existence, to expand our thinking about disorder in speech and hearing science, and to accept culturally competent communicators as competent communicators.
Application of Meta-Therapy to Dysphagia Rehabilitation
Authors: Donohue Cara , Coyle James L. 
Published: October 2022

Purpose:
Metatherapy is an important tool for practicing clinicians to implement when conducting dysphagia rehabilitation. Yet, few clinicians receive explicit training regarding how to implement metatherapy effectively with patients. As such, metatherapy skills are typically developed implicitly over time with experience. This tutorial will provide an overview of what metatherapy is and will provide instructions regarding how to implement metatherapeutic techniques for dysphagia rehabilitation by providing readers with a rehabilitation framework, case study examples, and techniques for conducting effective metatherapy.

Conclusion:
Dysphagia practitioners may use the framework and techniques provided in this tutorial to effectively implement metatherapy into dysphagia rehabilitation to enhance patient engagement in the therapy process and to improve patient outcomes and quality of life.