bluuebunny/crossref_metadata_2025_split huggingface dataset:
Usage of green pea aquafaba modified with ultrasound in production of whipped cream
Authors: KILIÇLI Mahmut , ÖZMEN Duygu , BAYRAM Mustafa , TOKER Omer Said 
Published: May 2022

Abstract
The objective of this study was to investigate the effect of the ultrasound on the functionality of green pea aquafaba (GPA). Ultrasound (20 kHz) (US) was applied at different levels and durations (50%, 200 W; 75%, 310 W and 100%, 430 W for 10, 20 and 30 mins). Foaming capacity (FC), foaming stability (FS), emulsifying activity index (EAI), SDS-PAGE and the rheological properties of whipped cream were determined on the ultrasound applied products. Central composite response surface design was applied and mathematical model was derived to explain the change in the samples. It was found that foaming capacity of green pea aquafaba increased from 575 to 725 % by increasing ultrasound level from control to 100% for 20 min. In addition, foaming stability increased except at 100% US level for 30 mins. A negative effect was observed in the emulsion activity index. No significant change (P>0.05) was obtained from the electrophoresis profiles from the control sample at 100% US for 10 min. It was also determined that ultrasound broke up protein structure at 100% US level for 15.46, 20 and 30 mins. According to the rheological results, the consistency coefficient (K’) and flow behavior index values (n’) of egg white, optimum and control whipped cream samples were determined as 17.8, 8.1 and 4.6 Pa.sn and 0.38, 0.46 and 0.51, respectively. In addition, the frequency results of whipped cream samples, the K’ and n’ values egg white, optimum and control whipped cream samples 617, 217 and 184 Pa.sn and 0.11, 0.23 and 0.21, respectively (R2=0.980).
Implementation components and factors that influence the provision of tele-audiology services for children and adults - a mixed-methods approach
Authors: Ramkumar Vidya , Shankar Varsha 
Published: May 2022

Abstract

Background
Tele-audiology has accelerated with the rapid advancement in information and communication technology. There is a significant knowledge gap concerning the use of implementation science and factors influencing tele-audiology as a routine or long-term, sustained effort. This mixed-methods study aimed to identify implementation factors that influence the provision of tele-audiology services.
Method
We carried out a mixed-methods study comprising a scoping review and semi-structured interviews (SSI). Articles describing tele-audiology were included based on a set of inclusion and exclusion criteria.
Results
Data was extracted from 32 studies that were mapped to 21 projects in tele-audiology. The broad areas of focus identified in tele-audiology included the provision of hearing screening, diagnostic evaluation, a combination of diagnostic audiological with otorhinolaryngological evaluation, hearing aid fitting and programming, cochlear implant fitting and mapping, aural re/habilitation services and comprehensive audiological services. Provision of diagnostic and therapeutic services were predominantly carried out using synchronous/ real-time telepractice methods. Asynchronous methods were used predominantly for audiometric screening, video-otoscopy and tympanometry. The most commonly used model was the ‘professional-facilitator-patient’ followed by the ‘professional-patient’ model. Barriers to long-term sustainability included insufficient initial financial investment, lack of reimbursement policies, limited internet (speed and bandwidth), and lack of organisational policies and uniform laws. Successful implementation was reported to be aided by having a stable source of funding, a devoted team of professionals and technicians with defined roles and duties, and with methodical planning.
Conclusion
The scoping review, as well as the interviewees, represented tele-audiology implementation efforts in a wide range of countries that include low-middle income and high-income nations. Successful implementation was reported to be aided by having a stable source of funding, a devoted team of professionals and technicians with defined roles and duties, and with methodical planning. Sustained implementation occurred when the need and usefulness of tele-audiology were accepted by public sector policy-makers. However, a more accurate understanding of sustainability can be obtained by the use of implementation science and frameworks to guide tele-audiology services.
A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis
Authors: Palmieri Dario , Capece Marina , Tessari Anna , Mills Joseph , Vinciguerra Gian Luca Rampioni , Lin Chenyu , McElwain Bryan , Miles Wayne , Coppola Vincenzo , Croce Carlo 
Published: May 2022

Abstract
The OsTIR1/auxin-inducible degron (AID) system allows selective protein degradation upon exposure to the phytohormone auxin. However, this technology does not allow to study the effect of acute protein depletion selectively in one phase of the cell cycle. Here, we report a new AID system to Regulate OsTIR1 Levels based on the Cell Cycle Status (ROLECCS) for phase-specific target proteolysis. Finally, we applied the ROLECCS technology to show that the tumor suppressor TP53 plays a S/G2-specific role in suppression of micronuclei accumulation. This new tool allows the analysis of different protein functions during cell cycle progression with unprecedented temporal resolution.
Photocatalytic degradation of reactive black dye using ZnO– CeO2 Nanocomposites
Authors: Saravanan Murugesan , Vigneshwar Sasibabu , Jegadeesan Gautham B. , Ponnusami Venkatachalam 
Published: May 2022

Abstract
Nano-CeO2 was synthesized via the chemical precipitation of cerium precursor solution, and then mixed with nano-ZnO at various weight ratios to obtain ZnO-CeO2nanocomposites. The composites were characterized for their morphological and photocatalytic properties. X-ray diffraction patterns of the pristine metal oxides corresponded well with (1 0 1) and (1 1 1) peaks of hexagonal wurtzite like-ZnO and cubic-phase CeO2, respectively. The band gap of the ZnO-CeO2nanocomposite was 3.08 eV, while that of pristine CeO2 and ZnO powder was 3.24 eV and 3.12 eV respectively. Photocatalytic activity of ZnO-CeO2composite was evaluated at various Reactive Black (RB) dye and catalyst concentrations. A 1:1 wt ratio ZnO-CeO2 nanocompsite provided the maximum (~ 85%) RB oxidation under UV light within 90 minutes. Rate of dye degradation obtained with ZnO-CeO2nanocomposite was almost 1.5 times more than that obtained with bare ZnO. It was observed that increase in CeO2 to ZnO ratio increased oxidation rates up to 1:1 wt ratio. Increasing CeO2 ratio beyond 1:1 wt. ratio did not significantly increase RB oxidation. The results confirm that addition of CeO2 to ZnO has resulted in lowering its bandgap energy and in-turn favors oxidation of RB dye under UV light.
Methylation of p53-responsive oncosuppressive microRNA genes in hemoblastosis
Authors: Voropaeva E. N. , Pospelova T. I. , Berezina O. V. , Churkina M. I. , Gurazheva A. A. , Maksimov V. N. 
Published: May 2022

The purpose of the study was to present up-to-date data on the frequency and significance of a number of p53-responsive oncosuppressive micrornas genes methylation in malignant neoplasms of the blood system.Material and methods. The search for available literary sources published in the Pubmed and RISC databases was carried out. A total of 399 articles were found, of which 62 were included in this review.Results. The p53 protein regulates a whole class of microRNAs – highly conserved small RNA molecules that affect gene expression mainly by suppressing translation. МicroRNAs play an important role in all cellular processes and can have both oncosuppressive and pro-oncogenic properties. Impaired expression of p53-activated oncosuppressive micrornas in various tumors may be associated with specific epigenetic mechanisms (DNA methylation and histone deacetylation). The review examines the molecular and genetic characteristics of oncosuppressive micrornas functioning in normal hematopoiesis, the violation of expression of which is shown in the development of hemoblastoses, namely: miR-34a, miR-34b/c, miR-145, miR-143 and miR-203. It is known that the transcription of the genes of these microRNAs is carried out and regulated from their own promoters. The latest published research results on the diagnostic, prognostic and clinical significance of gene methylation of the microRNAs under consideration in malignant neoplasms of the blood system are presented. According to literature data, common targets for mir-34a, mir-34b/c, mir-145, mir-143 and miR-203 microRNAs are mRNAs of a number of pro-oncogenes, namely: transcription factor C-MYC, positive cell cycle regulators at the G1/S transition point of CDK4, CDK6 and CYCLIN-D1 phases, anti-apoptotic proteins MDM2, MDM4, BCL2 and MCL1, as well as DNMT3A and DNMT3B methyltransferases and other molecules. In this regard, it should be noted that there are positive feedbacks between p53 and microRNAs activated by it, as well as negative feedbacks between p53-responsive micrornas and C-MYC and DNA methyltransferases.Conclusion. Thus, the data presented in the review clarify the current understanding of the work of the regulatory network of the p53 protein and the micrornas activated by it, and also emphasize the functional association of p53-responsive microRNAs.
Methods of creation of bladder cancer models and their application in preclinical studies (literature review)
Authors: Kamaeva I. A. , Goncharova A. S. , Lukbanova E. A. 
Published: May 2022

Purpose of the study to summarize available data on methods for creating bladder cancer models for their application in preclinical studies.Material and methods. A systematic literature search was conducted in the Elibrary, Pubmed, Googlescholar, CyberLeninka databases.Results. The review shows current data on various bladder cancer models and their application in practice. Bladder cancer pathology, identification of diagnostic markers and the development of new therapies are of the main challenges facing the management of bladder cancer. To solve these problems, it is often necessary to conduct preclinical studies using experimental models.Conclusion. Bladder cancer models that can fully reproduce a human disease in terms of histology and behavior are necessary to study the factors involved in cancer development, progression and metastasis. For this, various experimental models are currently used. Human tumor xenografts in mice are widely used. They can reproduce the main pathophysiological features of cancer biology. However, it is necessary to clearly present all the pros and cons of the selected experimental models. The literature review presents modern data on the etiology of bladder cancer, results of preclinical studies on various experimental models, including orthotopic and heterotopic xenografts.
Water and sanitation services in India and Ghana: an assessment of implications for rural health and related SDGs
Authors: Kanyagui Martin Kofi , Viswanathan P. K. 
Published: May 2022

Abstract
Provisioning of water and sanitation services has become one of the key determinants of SDGs. This review focuses on the trends in water and sanitation services and reforms in India and Ghana over the last two decades. The findings reveal that access to water has improved in India and Ghana at 81.5 and 92.7%, respectively. However, access to sanitation continues to be a challenge in both countries, with the currently reported coverage being 59.5% and 18.5%, respectively. The index of sustainable development goal (SDG) performance of Ghana and India stands at 65.4 and 61.9 with global rankings of 100 and 117, respectively. The adverse impacts of poor access to sanitation increasingly reflect on rising numbers of population suffering from water-borne diseases. From the policy perspective, the paper highlights the need for framing pro-poor water and sanitation policies; focusing on women and girls’ education; promoting affordable water and sanitation services; promoting collaboration of stakeholders involved in the rural water and sanitation sectors; and increasing budgetary allocations by local governments.
Cuidar de lesão crônica: saberes e práticas de pessoas com úlcera venosa
Authors: Vieira Isabelly Christina Gomes , Franzoi Mariana André Honorato 
Published: May 2022

Objetivo: Descrever saberes e práticas realizadas por pessoas com úlcera venosa no cuidado da lesão. Método: Estudo descritivo, de abordagem qualitativa, realizado com onze pacientes em tratamento de úlcera venosa em um Serviço Ambulatorial de Estomaterapia em Brasília, Distrito Federal. A coleta de dados foi realizada entre abril e maio de 2019 por meio de entrevistas semiestruturadas, que foram gravadas e posteriormente transcritas na íntegra. Os dados foram submetidos à análise de conteúdo temática. Resultados: A maioria era do sexo feminino e com mais de sessenta anos. Três categorias emergiram a partir da análise de conteúdo das entrevistas: práticas e saberes gerais no autocuidado da úlcera; mitos e crenças que perpassam o autocuidado; e dificuldades vivenciadas durante o cuidado com a úlcera. Conclusão: Este estudo desvelou saberes, práticas e também crenças que os pacientes com úlcera venosa apresentam a respeito da doença vascular e do processo de cicatrização da lesão, os quais propiciam melhor direcionamento no planejamento e no desenvolvimento de ações educativas em saúde voltada às necessidades dos usuários do ambulatório de forma a potencializar o autocuidado.
Caracterização clínica e epidemiológica de idosos de uma instituição de longa permanência
Authors: Bernardes Tatielle Aparecida Almeida , Santana Edileuza Teixeira , Coutinho Gabriella Gonçalves , Camisasca Luiza Rodrigues , De Araújo Gabriel Dias , Fagundes Pereira Fabíola Afonso , Maia Gusmão Ricardo Otávio , De Araújo Diego Dias 
Published: May 2022

Objetivo: Caracterizar clínica e epidemiologicamente os idosos residentes em uma instituição de longa permanência para idosos no Norte de Minas Gerais, Brasil. Método: Estudo descritivo e transversal realizado com a população idosa residente em uma instituição de longa permanência. Foi realizada entrevista individual com a aplicação de um instrumento de caracterização sociodemográfica, a Escala Katz e Teste do Relógio, no período entre 2018 e 2019. Os dados foram submetidos à análise estatística descritiva. Resultados: Participaram do estudo 116 idosos com média de idade de 78,240 (±9,21) anos, a maioria do sexo masculino (n=70; 60,3%), raça parda (n=74; 63,8%) e solteiros (n=77; 66,4%). Quanto aos resultados da Escala de Katz e Teste do Relógio, os participantes foram classificados como mais funcionais (n=39; 33,6%) e com inabilidade (n=90; 77,6%) respectivamente. Conclusões: A caracterização do perfil e o levantamento de problemas reais e potenciais contribuíram para o cuidado com os idosos que precisam ser apropriados e direcionados para que os idosos alcancem a máxima qualidade de vida, independência e funcionalidade.Descritores: Instituição de Longa Permanência para Idosos, Saúde do Idoso Institucionalizado, Idoso.
Sobrevida e fatores associados à mortalidade de pacientes com internações de longa permanência
Authors: De Paulo Magno Fernando , Vannuchi Marli Terezinha Oliveira , Rossaneis Mariana Angela , Haddad Maria do Carmo Fernandes Lourenço Lourenço , Fernandes Karen Barros Parron , Pissinati Paloma de Souza Cavalcante 
Published: May 2022

Objetivo: Analisar a sobrevida e os fatores associados à mortalidade de pacientes com internações de longa permanência. Método: Estudo transversal, a partir de registros dos pacientes que tiveram internação de longa permanência, em hospital de alta complexidade, pelo Sistema Único de Saúde, de 2014 a 2017, com exclusão das reinternações. Utilizou-se a Regressão de Cox para identificação dos fatores associados à mortalidade. Dentre os pacientes internados na Unidade de Terapia Intensiva aplicou-se a curva Roc para determinar o ponto de corte do dia de maior risco de óbito. Resultados: Foram identificadas 1.209 internações de longa permanência resultando em prevalência de 7,3%. Do total de pacientes de longa permanência, 50,3% foram a óbito, a maioria com idade superior a 60 anos, com doenças do aparelho circulatório (40%). Os fatores associados à mortalidade dos pacientes com internação de longa permanência foram: ser idoso (HR=2,31; IC95%:1,89-2,81; p<0,001); internação clínica (HR=1,82; IC95%: 1,54-2,15; p<0,001) e internação em UTI (HR=12,41; IC95%: 6,74-22,8; p<0,001). A mortalidade dos pacientes que foram internados na Unidade de Terapia Intensiva foi significativamente maior a partir do nono dia (p = 0,036). Conclusão: Verificou-se uma alta taxa de mortalidade em pacientes com internação de longa permanência, principalmente, entre idosos com doenças crônico-degenerativas e em cuidados paliativos.
Enfermeiros da área perioperatória no período da pandemia: aspectos institucionais e atitudinais
Authors: Rocha Gisele Dos Santos , Teixeira Elizabeth , Queiroz Eidie Souza de , Schuengu Cinthia Mara de Oliveira Lobato , Silva Alex Mariano Rosa da , Sabino Aderlaine Da Silva , Almeida Mailma Costa de 
Published: May 2022

Objetivo: analisar aspectos institucionais e atitudinais que emergem da atuação de enfermeiros da área perioperatória no período da pandemia do coronavírus. Método: estudo transversal descritivo, realizado no Amazonas, Pará e Roraima, Brasil. A coleta foi feita com 36 enfermeiros que atuam na área perioperatória, realizada em ambiente virtual, com aplicação de questionário entre os meses de julho e agosto de 2020. A análise foi por meio da estatística descritiva. Resultados: sobre a oferta de condições para o cumprimento das recomendações e normas somente quanto ao recebimento de EPI houve pleno atendimento. Sobre as atitudes dos enfermeiros diante das recomendações e normas não houve pleno cumprimento. Sobre estar atuando em contexto de pandemia, tiveram que fazer alterações na rotina e sentiram medo e insegurança. Conclusão: evidenciou-se que tanto na perspectiva institucional como atitudinal não se atingiu plenamente o estabelecido nas recomendações e normas, sendo importante a implementação de estratégias de educação permanente.
Modelos de Formação para a Atenção Primária à Saúde: evidências no contexto do Ensino de Enfermagem
Authors: Alvarenga José da Paz Oliveira , Leandro Suderlan Sabino , Soares Nathálya Silveira , Guimarães Daniela Mendes dos Santos , Silva Bianca Evellyn Santana , Soares Agatha Maria Teles , Mendonça Ana Valéria Machado , De Sousa Maria Fátima 
Published: May 2022

Objetivo: Analisar modelos de formação de enfermeiros na Atenção Primária à Saúde na literatura, considerando publicações de 2009 a 2019. Método: Scoping Review nas bases PubMed; BVS; ProQuest; EMBASE e Scielo. Os critérios de inclusão integram artigos em português, inglês, francês e espanhol. E como exclusão, ausência de resumos, artigos duplicados e não relacionados à formação de enfermagem na Atenção Primária. Resultados: Dos 369 artigos, 28 discutiam a formação de saúde para a atenção primária, em diferentes profissões. Dentre estes, 07 estavam relacionados à formação em enfermagem para a APS. Predominou o nível de evidências VII, classificando-os como estudos oriundos de relatório de comitês de especialistas. Conclusões: As evidências reafirmam o fortalecimento e a implementação da Educação Interprofissional, como uma abordagem de ensino que qualifica o processo de formação em enfermagem, valorizando as competências colaborativas. Conclui-se que há preocupação dos pesquisadores, de que o ensino de Enfermagem se desenvolva na perspectiva da formação, onde os estudantes possam desenvolver consciência profissional crítico-política para atuação na APS, com capacidade resolutiva na saúde pública, a partir das competências adquiridas. Descritores: Atenção Primária à Saúde; Formação em Saúde; Ensino de Enfermagem.
Complexidade das Práticas da Enfermagem na Atenção Primária à Saúde
Authors: De Sousa Maria Fátima , Dos Santos Betania Maria Pereira , Paz Elisabete Pimenta Araújo , Alvarenga José da Paz Oliveira 
Published: May 2022

Objetivo: Refletir sobre a complexidade das práticas de Enfermagem no âmbito da Atenção Primária à Saúde no Brasil, tendo na Estratégia Saúde da Família a base estruturante de um novo modelo de atenção. Método: Trata-se de um ensaio teórico-reflexivo, desenvolvido entre junho e julho de 2021, a paritr do conhecimento e das experiências, acadêmicas e profissionais dos autores; os quais analisam, refletem e contextualizam, sobre a contribuição da Enfermagem brasileira na extensão e profundidade dos atributos essenciais e derivados da APS, enraizados nos valores, princípios e diretrizes de promoção, prevenção, tratamento, reabilitação e cura nos processos de saúde-doença-cuidado. Resultados: Aponta os desafios contemporâneos à superação dos elementos constituintes do modelo médico-hospitalar, caracterizado pela ineficiência, baixa efetividade e insatisfação da população, em territórios de profundas desigualdades sociais, econômicas, políticas e sanitárias. Conclusão: Em síntese, indica que se faz necessário seguir imprimido movimentos ativos que ampliem as decisões políticas de assegurar a saúde como direito de cidadania plena, portanto como um bem fundamental do ser humano. Descritores: Estado; Sistema Único de Saúde; Atenção Primária à Saúde; Estratégia Saúde da Família; Práticas de Enfermagem.
Anxiolytic and Antidepressant-like Effects of Monoterpene Tetrahydrolinalool andIn silicoApproach of new Potential Targets
Authors: Rayff da Silva Pablo , Diniz NunesPazos Natalia , Karla Silva do Nascimento Gonzaga Thallita , Cabral de Andrade Jéssica , Brito Monteiro Álefe , Caroline Ribeiro Portela Anne , Fernandes Oliveira Pires Hugo , dos Santos Maia Mayara , Vilar da Fonsêca Diogo , T. Scotti Marcus , Maria Barbosa Filho José , Pergentino de Sousa Damião , Francisco Bezerra Felipe Cícero , Nóbrega de Almeida Reinaldo , Scotti Luciana 
Published: May 2022

Introduction:Although drugs currently available for the treatment of anxiety and de-pression act through modulation of the neurotransmission systems involved in the neurobiology of the disorder, yet they often present side effects, which can impair patient adherence to treatment.Methods:This has driven the search for new molecules with anxiolytic and antidepressant potential. Aromatic plants are rich in essential oils, and their chemical constituents, such as monoterpenes, are being studied for these disorders. This study aims to evaluate the anxiolytic and antidepressant-like potential of the monoterpene tetrahydrolinalool in in vivo animal models and review pharmacologi-cal targets with validation through molecular docking. Male Swiss mice (Mus musculus) were treat-ed with THL (37.5-600 mg kg-1 p.o.) and submitted to the elevated plus maze, open field, rotarod, and forced swim tests. In the elevated plus-maze, THL at doses of 37.5 and 75 mg kg-1 induced a significant increase in the percentage of entries (72.7 and 64.3% respectively), and lengths of stay (80.3 and 76.8% respectively) in the open arms tests.Results:These doses did not compromise locomotor activity or motor coordination in the animals. In the open field, rotarod tests, and the forced swimming model, treatment with THL significantly reduced immobility times at doses of 150, 300, and 600 mg kg-1, and by respective percentages of 69.3, 60.9 and 68.7%.Conclusion:In molecular docking assay, which investigated potential targets, THL presented satis-factory energy values for: nNOs, SGC, IL-6, 5-HT1A, NMDAr, and D1. These demonstrate the po-tential of THL (a derivative of natural origin) in in vivo and in silico models, making it a drug can-didate.