bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphoglycerate mutase 1 that is essential for glycolysis may act as a novel metabolic target for predicating poor prognosis for patients with gastric cancer
Authors: Wei Chen , Xie Jiebin , Yuan Xiaoxia , Luo Yaomin , Xiao Yang , Liao Weiliang , Jiang Zhen 
Published: October 2022

AbstractBackgroundTo identify a novel marker for gastric cancer, we examined the usefulness of phosphoglycerate mutase 1 (PGAM1) as a potential diagnostic marker using isobaric tags for relative and absolute quantitation (iTRAQ)‐based quantitative proteomics and evaluated its clinical significance.MethodsProteins from a discovery group of four paired gastric cancer tissues and adjacent gastric tissues were labeled with iTRAQ reagents and then identified and quantified using LC–MS/MS. The expression of PGAM1 was further validated in 139 gastric cancer patients using immunohistochemistry. Furthermore, the correlation of PGAM1 expression with clinical parameters was analyzed. Gene set enrichment analysis (GSEA) was performed to identify gene sets that were activated in PGAM1‐overexpressing patients with gastric cancer.ResultsPGAM1 was significantly overexpressed in most cancers but particularly so in gastric cancer, with a sensitivity of 82.01% (95% confidence interval [CI]: 75.5%–88.5%) and specificity of 79.13% (95% CI: 72.3%–86%). Its expression was significantly associated with histological grade II and III tumors (p = 0.033), lymph node metastasis (p = 0.031), and TNM III‐IV staging (p = 0.025). The area under the receiver operating characteristic (ROC) curve for the detection of PGAM1 overexpression in gastric cancer was 0.718 (p < 0.01). Furthermore, GSEA revealed that several important pathways such as glycolysis pathway and immune pathways were significantly enriched in patients with gastric cancer with PGAM1 overexpression.ConclusionsThis study provided a sensitive method for detecting PGAM1, which may serve as a novel indicator for poor prognosis of gastric cancer, as well as a potent drug target for gastric cancer.
Downregulation of MMP1 functions in preventing perineural invasion of pancreatic cancer through blocking the NT‐3/TrkC signaling pathway
Authors: Xu Xiaoqing , Lu Xiaomin , Chen Liping , Peng Ke , Ji Fuhai 
Published: October 2022

AbstractBackgroundPancreatic cancer (PC) is a fatal malignancy that frequently involves perineural invasion (PNI). This study aims to investigate the function and underlying mechanisms of matrix metalloproteinase‐1 (MMP1) in PNI of PC.MethodsHuman pancreatic cancer PANC‐1 cells were co‐cultured with dorsal root ganglion in vitro. The expression of MMP1, epithelial–mesenchymal transition (EMT) markers, Schwann cell markers, neurotrophic factors, NT‐3, and TrkC was measured by qRT‐PCR or Western blot. Transwell assay was performed to evaluate cell migration and invasion. In vivo model of PNI was established via inoculating PANC‐1 cells into mice. PANC‐1 cells and mice were also treated with LM22B‐10 (an activator of TrkC) to confirm the mechanisms involving NT‐3/TrkC in PNI of PC both in vivo and in vitro.ResultsThe expression of MMP1 was significantly higher in PDAC tissues than non‐cancerous tissues, which was positively associated with PNI. MMP1 knockdown repressed the migration and invasion of PANC‐1 cells. Except for E‐cadherin, the expression of EMT markers, Schwann cell markers, neurotrophic factors, NT‐3, and TrkC was inhibited by MMP1 silencing. The same effects of MMP1 knockdown on the above factors were also observed in the PNI model. Moreover, MMP1 knockdown elevated the sciatic nerve function and reduced PNI in the model mice. LM22B‐10 partially abolished the effects of MMP1 knockdown both in vivo and in vitro.ConclusionsSilencing of MMP1 prevents PC cells from EMT and Schwann‐like cell differentiation via inhibiting the activation of the NT‐3/TrkC signaling pathway, thus alleviating the PNI of PC.
Exploration of a predictive model based on genes associated with fatty acid metabolism and clinical treatment for head and neck squamous cell carcinoma
Authors: Yuan Zhechen , Huang Juntao , Teh Bing Mei , Hu Shiyu , Hu Yi , Shen Yi 
Published: October 2022

AbstractBackgroundHead and neck squamous cell carcinoma (HNSCC) is one of the most prevalent malignant tumors of the head and neck and presents high risks of recurrence and poor prognosis postoperatively. The aim of this study was to establish a predictive model based on fatty acid metabolism (FAM) genes to forecast the prognosis of HNSCC patients and the subsequent treatment strategies.MethodsWe accessed the TCGA and GEO databases for HNSCC genes and clinical data. The FAM risk score model was created and validated using a combination of univariate Cox analysis and least absolute shrinkage and selection operator (LASSO) regression analysis. Combining risk scores and clinical characteristics, a nomogram was established and assessed. Subsequently, the function, gene mutation, immune difference, and chemotherapeutic drug sensitivity of the groups with high‐ and low‐risk scores were analyzed. Consequently, the mode's validity was evaluated comprehensively by combining single gene analysis.ResultsThe FAM risk score model for predicting HNSCC prognosis had certain validity. Patients in the high‐ and low‐risk groups had genetic mutations, and the prognosis was the poorest for the high‐risk groups with high genetic mutations. The patients with low‐risk scores were suitable for immunotherapy since they had increased infiltration of immune cells. In contrast, the patients in the other groups were more suitable for chemotherapy.ConclusionThe results of this study demonstrated that the FAM risk score model may predict the prognosis of HSNCC and has a certain therapeutic guidance value.
The CHST11 gene is linked to lung cancer and pulmonary fibrosis
Authors: Li Chien‐Hsiu , Chan Ming‐Hsien , Chang Yu‐Chan , Hsiao Michael 
Published: October 2022

AbstractBackgroundThe abnormal modification of chondroitin sulfate is one of the leading causes of disease, including cancer progression. During chondroitin sulfate biosynthesis, the CHST11 enzyme plays a vital role in its modification, but its role in cancer is not fully understood. Therefore, understanding the relationship between CHST11 and pulmonary‐related diseases through clinically relevant information may be useful for diagnosis or treatment.MethodsA variety of pulmonary fibrosis clinical gene expression omnibus (GEO) datasets were used to assess the association between CHST11‐related manifestations and fibrosis. Multiple lung cancer‐related databases, including The Cancer Genome Atlas, GEO datasets, UCSC Xena, GEPIA2, Cbioportal and ingenuity pathway analysis were used to evaluate the clinical correlation between CHST11 and lung cancer and potential molecular mechanisms. For drug repurposing prediction, the molecules that correlated with CHST11 were subjected to the LINCS L1000 algorithm. A variety ofin vitroassays were performed to evaluate thein‐silicomodels, including RNA and protein expression, proliferation, migration and invasion.ResultsClinical analyses indicate that the levels of CHST11 are significantly elevated in cases of pulmonary‐related diseases, including fibrosis and lung cancer. According to multiple lung cancer cohorts, CHST11 is the only member of the carbohydrate sulfotransferase family associated with overall survival for lung adenocarcinomas, and it is highly related to smoking‐induced lung cancer patients. Based on the results ofin vitroexperiments, CHST11 expression contributes to tumor malignancy and promotes multiple fibrotic activators. Correlation‐based ingenuity pathway analysis indicated that CHST11‐related molecules contributed to pulmonary fibrosis or lung adenocarcinomas via similar upstream stimulators. Based on known molecular regulatory relationships, CHST11 has been associated with the regulation of TGF‐βand INFγas important molecules contributing to fibrosis and cancer progression. Interestingly, WordCloud analysis revealed that CHST11‐related molecules are involved in regulation primarily by integrin signaling, and these relationships were consistently reflected in the analysis of cell lines and the clinical correlation. A CHST11 signature‐based drug repurposing analysis demonstrated that the CHST11/integrin axis could be targeted by AG‐1478 (Tyrphostin AG 1478), brefeldin A, geldanamycin and importazole.ConclusionsThis study provides the first demonstration that CHST11 may be used as a biomarker for pulmonary fibrosis or lung cancer, and the levels of CHST11 were increased by TGF‐βand INFγ. The molecular simulation analyses demonstrate that the CHST11/integrin axis is a potential therapeutic target for treating lung cancer.
The long non‐sacoding RNA TMEM147‐AS1/miR‐133b/ZNF587 axis regulates the Warburg effect and promotes prostatic carcinoma invasion and proliferation
Authors: Wu Tao , Han Niwei , Zhao Changyong , Huang Xiang , Su Peng , Li Xiaoguang 
Published: October 2022

AbstractBackgroundThe Warburg effect is a characteristic tumor cell behavior regarded as one of the cancer hallmarks and promotes tumor progression by promoting glucose uptake and lactate production. Long non‐coding RNAs (lncRNAs) had been reported to emerge as a vital function in cancer development. The present research is designed to investigate the underlying molecular mechanism of lncRNA TMEM147 antisense RNA 1 (TMEM147‐AS1) on aerobic glycolysis in prostatic carcinoma.MethodslncRNA TMEM147‐AS1, miR‐133b and ZNF587 levels in prostatic carcinoma tissues and cells were detected by a polymerase chain reaction or western blot assays. Cell viability or invasion was determined by Edu (i.e. 5‐ethynyl‐2′‐deoxyuridine), MTT (i.e. 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyl tetrazolium bromide) or transwell assays. Hematoxylin and eosin and immunohistochemical staining were applied for histopathological examination. Tumor xenograft model was employed to investigate tumor growth in vivo. The combinative relationship between TMEM147‐AS1 or ZNF587 and miR‐133b was confirmed by a luciferase reporter assay.ResultsTMEM147‐AS1 and ZNF587 were up‐regulated in prostatic carcinoma tissues and cells. Knockdown of TMEM147‐AS1 or ZNF587 within prostate cancer cells significantly restrained cell viability, invasion and aerobic glycolysis in vitro and suppressed the neoplasia of prostatic carcinoma in vivo. miR‐133b was directly targeted in both TMEM147‐AS1 and ZNF587. Overexpression of miR‐133b restrained prostate cancer cell viability, invasion and aerobic glycolysis. TMEM147‐AS1 competitively targeted miR‐133b, therefore counteracting miR‐133b‐mediated repression on ZNF587.ConclusionsTMEM147‐AS1 plays a tumor‐promoting action in prostatic carcinoma aerobic glycolysis via affecting the miR‐133b/ZNF587 axis, therefore regulating prostatic carcinoma cells invasion and proliferation. These outcomes implied that TMEM147‐AS1 could be an effective treatment strategy for further study of prostatic carcinoma.
The significance of osteosarcopenia as a predictor of the long‐term outcomes in hepatocellular carcinoma after hepatic resection
Authors: Yanagaki Mitsuru , Haruki Koichiro , Taniai Tomohiko , Igarashi Yosuke , Yasuda Jungo , Furukawa Kenei , Onda Shinji , Shirai Yoshihiro , Tsunematsu Masashi , Ikegami Toru 
Published: October 2022

AbstractBackgroundFrailty, represented by sarcopenia and osteopenia, has been associated with worse survival after hepatectomy in patients with hepatocellular carcinoma. Recently, the concept and term “osteosarcopenia” have been proposed, which has been associated with worse outcome in several malignancies.MethodsThis study included 227 patients who underwent elective hepatic resection for hepatocellular carcinoma. Osteopenia was evaluated with computed tomographic measurement of pixel density in the midvertebral core of the 11th thoracic vertebra. The skeletal muscle index (SMI) was evaluated with psoas muscle areas at the third lumbar vertebra. Osteosarcopenia was defined as the concomitant occurrence of osteopenia and low SMI. We retrospectively investigated the relationship between osteosarcopenia and disease‐free and overall survival.ResultsOsteosarcopenia was identified in 27 (12%) of the patients. Multivariate analysis showed that osteosarcopenia (P < .001), C‐reactive protein‐to‐albumin ratio ≥0.02 (P = .002), and stage ≥III (P ≤ .001) were independent predictors of disease‐free survival, while osteosarcopenia (P < .001), Child‐Pugh grade B (P = .002), C‐reactive protein‐to‐albumin ratio ≥0.02 (P = .001), low SMI (P ≤ .001), and stage ≥III (P ≤ .001) were independent predictors of overall survival.ConclusionPreoperative osteosarcopenia may be a prognostic factor in patients with hepatocellular carcinoma after hepatic resection. Our results suggests that the preoperative maintenance of skeletal muscle and bone density may potentially improve prognosis.
Changes in Hungarian academic psychology after the end of “people's democracy”
Authors: Pléh Csaba 
Published: October 2022

AbstractThe paper surveys the last 30 years of Hungarian academic psychology. Around 1989–1990, the time of the great social changes Hungarian psychology was rather Westernized, but still a relatively small scientific field and applied profession. The opening and liberalization of politics made psychology in Hungary a booming profession and a rich research field. Education of psychologists was spreading, and becoming more Westernized in textbook usage and reading materials. Entrance numbers at two universities with 80 students were replaced by 2010 by 6 university programs and about 8000 incoming students. The training system is a Bologna type BA + MA + PhD system, The educational booming has its own problems. As all university subjects, psychology training is also underfinanced, with high teaching loads and a move by university management towards applied areas, neglecting basic research. The research activity is characterized by a fivefold increase of English language publications coming from Hungary over a 20 years period. University research was strengthened, and competitive grant systems were introduced, whth good success aretes by psychologists. Here again, managerial thinking questions many aspects of basic research and liberalized science management. These factors are peculiar to psychology, but they do have an impact on it. The paper gives some details about one chapter of academic psychology, cognitive psychology. Institutionally, support by the Soros foundation in the 90s for the university cognitive programs had as one consequence that three departments of cognition are active in Budapest today. Another aspect of insitutional development was the series of multidisciplinary conferences in Hungary (MAKOG), and Hungarian involvement in international graduate training programs in cognitive science. The most successful cognitive group, at Central European University (5 ERC grants, publications in leading journals) is recently chased out of Hungary by anti‐Western and antiliberal legal moves. This would certainly have a detrimental effect on Hungarian cognitive psychology for quite a time.
Killing your children to hurt your partner: A South African perspective on the motivations for revenge filicide
Authors: Moen Melanie , Bezuidenhout Christiaan 
Published: October 2022

AbstractThe aim of the study was to investigate the offence characteristics and motivations for revenge filicides. Revenge filicide is an act where one parent kills their own offspring for retribution to hurt and upset the other parent. The cases of 20 revenge filicide murderers (14 male and six female) were analysed to determine the motivations and offence characteristics of revenge filicide offenders. It is the first South African study to highlight the motivations and associated characteristics in revenge filicides. Themes such as a loss of social identity due to rejection; extreme rage type anger; external locus of control; sadism; a desire to cause pain and a need to inflict harm are highlighted in this article. The initial emotional response may escalate from mild anger to a level of narcissistic rage which eventually culminates in the murdering of the child to punish and hurt the other parent and to restore control.
Rapid separation and identification of 96 main constituents in Huanglian Jiedu decoction via ultra‐high performance liquid chromatography–Orbitrap Fusion Tribrid mass spectrometer
Authors: He Dongmei , Liao Fengyun , Wang Peng , Gan Bing , Yu Lingling 
Published: October 2022

AbstractHuanglian Jiedu decoction is a widely used traditional Chinese medicine with a broad spectrum of therapeutic effects, including heat clearing, detoxification, and attenuation of inflammation. However, the composition of Huanglian Jiedu decoction is still unclear due to its complexity and limitations of analytical methods. In this study, we established a fast and reliable analytical method based on ultra‐performance LC–Orbitrap Fusion Tribrid mass spectrometer for high‐speed separation and structural identification of multiple compounds in Huanglian Jiedu decoction. The analysis was carried out using a Hypersil GOLD C18 column (2.1 × 100 mm, 1.9 μm) with gradient elution coupled to a high‐definition mass spectrometer system operating in both positive and negative ESI modes. According to the chromatographic retention time, precise molecular weight, fragment ion peaks, and published data, the main chromatographic peaks were attributed to specific molecules whose chemical structures were determined. In total, 96 components were identified, including 34 flavonoids and their glycosides, 23 alkaloids, 18 organic acids, 13 terpenoids, and 8 miscellaneous compounds. This study revealed the detailed chemical composition of Huanglian Jiedu decoction, which is of great importance for quality control and further pharmacological and mechanistic studies.
Thermal conductivity and phonon anharmonicity of chemical vapor transport grown and mineral–FeS2single crystals: An optothermal Raman study
Authors: Özden Ayberk , Zuñiga‐Puelles Esteban , Kortus Jens , Gumeniuk Roman , Himcinschi Cameliu 
Published: October 2022

AbstractIn this work, thermal conductivity and anharmonic properties of chemical vapor transport (CVT)‐grown pyrite‐FeS2and mineral single crystals have been investigated and compared. It has been shown that optothermal Raman technique is able to capture the large thermal conductivity difference between the CVT and mineral samples at low temperatures. This difference is attributed to point defects such as sulfur vacancies or impurity doping of CVT‐grown FeS2crystals. Balkanski–Klemens model analysis of the samples has shown that three‐phonon scattering of Agand Egmodes and the lattice thermal expansion are the dominant anharmonic contributors while four‐phonon scattering is negligible in pyrite‐FeS2. Thus, thermal conductivity of materials that is difficult to measure by conventional methods (i.e., flash method) can be measured in their most native form by using optothermal Raman spectroscopy (RS) without rigorous sample preparation.
N‐acetylcysteine attenuates oxidative stress‐mediated cell viability loss induced by dimethyl sulfoxide in cryopreservation of human nucleus pulposus cells: A potential solution for mass production
Authors: Tamagawa Shota , Sakai Daisuke , Schol Jordy , Sako Kosuke , Nakamura Yoshihiko , Matsushita Erika , Warita Takayuki , Hazuki Soma , Nojiri Hidetoshi , Sato Masato , Ishijima Muneaki , Watanabe Masahiko 
Published: October 2022

AbstractBackgroundCell therapy is considered a promising strategy for intervertebral disc (IVD) regeneration. However, cell products often require long‐term cryopreservation, which compromises cell viability and potency, thus potentially hindering commercialization and off‐the‐shelf availability. Dimethyl sulfoxide (DMSO) is a commonly used cryoprotectant, however, DMSO is associated with cytotoxicity and cell viability loss. This study aimed to investigate the effects of DMSO on human nucleus pulposus cells (NPC) and the role of oxidative stress in DMSO‐induced cytotoxicity. Furthermore, we examined the potential of antioxidantN‐acetylcysteine (NAC) supplementation to mitigate the negative effects of DMSO.MethodsNPC were exposed to various concentrations of DMSO with or without a freezing cycle. Cell viability, cell apoptosis and necrosis rates, intracellular reactive oxygen species (ROS) levels, and gene expression of major antioxidant enzymes were evaluated. In addition, NAC was added to cryopreservation medium containing 10% DMSO and its effects on ROS levels and cell viability were assessed.ResultsDMSO concentrations ≤1% for 24 h did not significantly affect the NPC viability, whereas exposure to 5 and 10% DMSO (most commonly used concentration) caused cell viability loss (loss of 57% and 68% respectively after 24 h) and cell death in a dose‐ and time‐dependent manner. DMSO increased intracellular and mitochondrial ROS (1.9‐fold and 3.6‐fold respectively after 12 h exposure to 10% DMSO) and downregulated gene expression levels of antioxidant enzymes in a dose‐dependent manner. Tempering ROS through NAC treatment significantly attenuated DMSO‐induced oxidative stress and supported maintenance of cell viability.ConclusionsThis study demonstrated dose‐ and time‐dependent cytotoxic effects of DMSO on human NPC. The addition of NAC to the cryopreservation medium ameliorated cell viability loss by reducing DMSO‐induced oxidative stress in the freeze–thawing cycle. These findings may be useful for future clinical applications of whole cells and cellular products.
The impact of an otolaryngology inpatient consult documentation improvement program
Authors: Virani Farrukh R. , Wickwire Peter C. , Aizenberg Debbie A. 
Published: October 2022

AbstractObjectiveIn an era of limited medical training funds and challenges for teaching centers to maintain their academic mission, the importance of accurate documentation to ensure commensurate coding and billing for services is critical. We sought to develop a practical program that would teach residents documentation skills with the goal of more accurately capturing the work being done in a tertiary care academic medical center.MethodsA case–control study was performed. Otolaryngology inpatient and Emergency Department consultation notes at a single tertiary medical center were reviewed and knowledge gaps and shortcomings in documentation identified. Three short educational sessions were provided on documentation skills. During the same timeframe, templates in the electronic medical record were standardized to help maintain thoroughness of documentation within the consultation note.ResultsA total of 1476 consultations performed by the Otolaryngology department during a 9‐month period in FY17/18 (preintervention) were compared to a total of 1622 consultations performed during the same 9‐month period in FY19/20 (postintervention). The percent of billable consultations increased from 42.4% to 50.9% (p < .001). Similarly, the percentage of consultations coding at a higher level of complexity rose from 51.6% to 59.5% (p = .002). This improvement led to an increase in consultation charges of more than $130,000.ConclusionThis study demonstrates that a simple documentation and coding curriculum and workflow interventions can lead to more thorough and improved consult documentation as evidenced by a significant increase in the percentage and complexity of billable Otolaryngology consultations at a tertiary academic center.Level of Evidence4.