bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacts of Carbonated Brine-Rock Reactions on Multiphase Flow Properties in Upper Minnelusa Sandstone: Implication for CO2 Storage
Authors: Kou Zuhao 
Published: September 2022

Abstract
The impact of carbonated brine-rock geochemical reactions on porosity, permeability, and multiphase flow responses is relevant to the determination of CO2 storage capacity of deep saline aquifers. In this research, carbonated brine flooding experiments were performed on core samples consisting of poorly sorted, quartz-rich sand with laminated bedding from a target CO2 storage formation in Wyoming. Complementary pre- and post-injection lab measurements were performed. Results showed that both core porosity and permeability increased after a seven-day carbonated brine injection, from 6.2% to 8.4% and 1.6mD to 3.7mD, respectively. These changes were attributed to carbonate mineral dissolution, which was evidenced by the effluent brine geochemistry, pore-throat size distribution and surface area. To be more specific, within the more permeable section of core samples, containing larger pore size, the permeability increment is apparent due to dolomite mineral grains and cements dissolution. However, for the lower permeability section, corresponding to the smaller pore size, mineral precipitation possibly lessened dissolution effects, leading to insignificant petrophysical properties changes. Consequently, the observed heterogeneous carbonated brine-rock interactions resulted in changes of CO2/brine relative permeability. This research provides a fundamental understanding regarding impacts of fluid-rock reactions on changes in multiphase flow properties of eolian sandstones, which lays the foundation for more accurate prediction/simulation of CO2 injection into deep saline aquifers.
Evaluation of the prognostic factors for pediatric intestinal motility disorders: A single institution experience
Authors: Yano Keisuke , Muto Mitsuru , Sugita Koshiro , Murakami Masakazu , Onishi Shun , Harumatsu Toshio , Yamada Koji , Yamada Waka , Matsukubo Makoto , Kawano Takafumi , Kaji Tatsuru , Ieiri Satoshi 
Published: September 2022

Abstract
Purpose
To improve the life prognosis of patients with intestinal motility disorder (IMD), we explored predictors of survival.
Methods
We reviewed the medical records of IMD patients who required total parenteral nutrition (TPN) for four weeks or more at our institution from April 1984 to November 2021. We retrospectively evaluated the characteristics of patients with IMD as predictive factors.
Results
Fourteen patients were enrolled. They had high mortality rate (50%). The types of IMD were as follows: hypoganglionosis, extensive aganglionosis, and chronic idiopathic intestinal pseudo-obstruction. The mortality did not differ to a statistically significant extent among patients with three types of IMD. A significant difference was observed in the length of enterostomy between survivors and the non-survivors (p = 0.028). Cholestasis was a significant prognostic factor (p = 0.005). Intestinal failure associated liver disease (IFALD) was the cause of death in the short term. Catheter-related blood stream infection (CRBSI) and acute rejection after small bowel transplantation were causes of death in the long term.
Conclusion
IMD patients still have a high mortality rate and cholestasis predicts survival in IMD patients. Preventing or improving IFALD and CRBSI due to long-term TPN is important for reducing the mortality rate.
Malted sorghum as a maize substitute in broiler diets: Effect on feed utilization, growth performance, and blood parameters
Authors: Moses Chandapiwa , manyeula Freddy , Malebogo Radikara V. , Mareko Molebeledi H.D. , Madibela Othusitse R. 
Published: September 2022

Abstract
Sorghum is amongst the most drought-tolerant cereals in the driest rain-fed regions of the arid and semi-arid tropics and hence its suitability in circumventing food and feed insecurity. However, the presence of phytochemicals may limit its utilization in sustainable poultry production which require some form of treatment before use. Thus, this study was conducted to explore malting as a processing method and its effect on growth performance and haemo-biochemical parameters of broiler chickens. Three isonitrogenous and isoenergetic diets were formulated by totally replacing maize with either malted Mr Buster (MBBD) or Segaolane (MSBD) and Control. One hundred and fifty one-week old Ross 308 broiler (R308BC) were evenly distributed into 15 replicate pens (10 birds/pen) to which experimental diets were randomly allocated and offered in three phase feeding. Birds fed MSBD were eating more than those fed other diets. The birds on Control gained more weight than those fed malted sorghum based (MSOBD). Poor feed conversion ratio (FCR) was observed on MBBD birds in week 3, 4, 5 and 6.The PER was lowest on MSOBD birds in week 2 and 6 but in week 5 the opposite is the truth. Protein intake (PI) of birds on MSOBD was highest in all weeks. Haematological and serum parameters fell within reported normal range of healthy chicken. Overall, the results from the study suggest that maize grains can be replaced with malted sorghum grains, especially Segaolane in poultry diets without any adverse effects on the bird’s growth’s performance and health status.
Small Sample Epilepsy Detection Method Based on Convolutional Prototype Learning
Authors: He Anqi , Lyu Chengang , Chen Zhijuan , Liu Yuheng , Li Jing , Gong Junjie , Zhao Mingyu , Yang Chen , Jin Jie , Wang Zengguang , Chen Yuxin 
Published: September 2022

Abstract
Background: Several scalp EEG epilepsy detection methods based on machine learning have achieved good detection accuracy. However, in clinical applications, different EEG acquisition equipment and experience of neurologists make the quality and style of EEG signals different, which makes previous epilepsy detection models cannot be widely used. The establishment of epilepsy detection model for a certain hospital usually depends on a large number of EEG samples, but there are usually few EEG samples from a certain hospital.
Methods: To solve this problem, we proposed a small sample epilepsy detection method based on convolutional prototype learning (CPL) in this paper. CPL consists of convolutional neural network (CNN) and prototype learning. CNN is used as an adaptive feature extraction algorithm, and prototype learning is used as a small sample classification algorithm.
Results: In the experiment, we select 20, 40, 60, 80, 100 and 120 samples to train and save 6 CPL-based detection models. The 6 models are used to classify the test samples, and the accuracy are 75.97%, 83.24%, 85.67%, 88.27%, 91.09% and 94.43% respectively.
Conclusions: The CPL can realize automatic feature extraction of EEG signals, and solve the problem of insufficient training samples in epilepsy detection.
BTB/POZ proteins and their gene family in Oryza rufipogon: Novel genes and alleles from the progenitor of the domesticated japonica rice (O. sativa L.)
Authors: Mandal Swarupa Nanda , Sanchez Jacobo , Bhowmick Rakesh , Bello Oluwatobi R . , Beek Coenraad Van , Benildo de los Reyes 
Published: September 2022

AbstractThe BTB/POZ family of proteins is widespread in plants and animals, playing important roles in development, growth, metabolism, and environmental responses. Although members of the expanded BTB/POZ gene family (OsBTB) have been identified in cultivated rice (Oryza sativa), their conservation, novelty, and potential applications for allele mining inO. rufipogon, the direct progenitor ofO. sativassp. japonica and potential wide-introgression donor, are yet to be explored. This study describes an analysis of 110 BTB/POZ encoding gene loci (OrBTB) across the genome ofO. rufipogonas outcomes of tandem duplication events. Phylogenetic grouping of duplicatedOrBTBgenes was supported by the analysis of gene sequences and protein domain architecture, shedding some light on their evolution and functional divergence. TheO. rufipogongenome encodes eight novel BTB/POZ genes with orthologs in its distant cousins in the family Poaceae (Sorghum bicolor, Brachypodium distachyon), but such orthologs appeared to have been lost in its domesticated descendant,O. sativassp. japonica. Comparative sequence analysis and structure comparisons of novelOrBTBgenes revealed that diverged upstream regulatory sequences and regulon restructuring are the key features of the evolution of this large gene family. Novel genes from the wild progenitor serve as a reservoir of potential new alleles that can bring novel functions to cultivars when introgressed by wide hybridization. This study establishes a foundation for hypothesis-driven functional genomic studies and their applications for widening the genetic base of rice cultivars through the introgression of novel genes or alleles from the exotic gene pool.
Factors influencing accumulation of the selected metals in the tissues of spiny-cheek crayfish (Orconectes limosus Raf.)
Authors: Stanek Magdalena , Chachaj Bogusław , Różański Szymon 
Published: September 2022

Abstract
The aim of the study was to analyse the effect of age and sex of crayfish on the degree of accumulation of selected metals in the meat and exoskeleton. A total of 50 males and 50 females of the spiny-cheek crayfish were caught in autumn (October 2019) after a period of intense feeding using fyke nets. Metal concentrations were determined in freeze dried samples of the abdominal meat, exoskeleton and bottom sediments and water using atomic absorption spectroscopy (AAS) with a SOLAR S4 spectrophotometer. The highest amounts of Zn were found in the meat of 4-year-old males, Cu of 3-year-old males, and Ca of 4-year-old males. As statistical calculations showed, both the age and sex of the crayfish were significant factors. BCF of Zn and Cu in the meat and exoskeleton of spiny-cheek crayfish was much higher from water than from sediments, unlike Ca. Only in a few cases the concentration of metals in the meat and exoskeleton significantly correlated with the total length (TL) of the crayfish. Consumption of 100 g of spiny-cheek crayfish meat could meet daily requirement for Zn up to 27.5% and for Ca in 12.4% and over 100% for Cu.
Polydatin Increases Lifespan and Rescue Lead-induced Behavioural Deficits, Inflammatory and Oxidative Damage in Drosophila melanogaster Harwich Strain.
Authors: Highab Salisu Muhammad , Ya'u Jamilu , Magaji Muhammad Garba , Shehu Dalhatu Muktar 
Published: September 2022

Abstract
Background
Lead (Pb) is toxic and cause many adverse clinical outcomes in children and adult, however, polydatin (PD) is a natural product from plants with reported antioxidative, neuroprotective and inflammatory properties. This study investigated the role of polydatin on lead-induced behavioural deficits, oxidative stress and inflammatory damages in D. melanogaster. D. melanogaster (Harwich strain, 1 to 3 days old) were orally administered lead acetate (PbAc) (0, 50, 100, 250 and 500 µM/5g diet) and PD (0, 5, 10, 20 and 40 µM/5g diet) for 14-days survival assays respectively. Thereafter, three concentrations of PD (10, 20 and 40 µM/5g diet) and one concentration of PbAc (250 µM/5g diet) were selected to evaluate the ameliorative potential of polydatin on PbAc-induced toxicity in D. melanogaster after 5-days oral co-treatment.
Results
Markers of behavioural (acetylcholinesterase, locomotor performance, fecundity and eclosure of the flies (emergence)), oxidative stress-antioxidant status (hydrogen peroxide, total thiol, catalase and glutathione-S-transferase, cell viability), inflammation (nitric oxide) were evaluated. Polydatin elevate the lifespan of D. melanogaster in a dose-dependent manner up to 40 µM/kg diet. Furthermore, polydatin alleviate PbAc-induced inhibition of catalase, glutathione-S-transferase and acetylcholinesterase activities in D. melanogaster. Moreover, polydatin significantly (p < 0.05) alleviate PbAc-induced cell death, behavioural deficits, accumulation of nitric oxide, hydrogen peroxide, total thiol levels and histopathological lesions in flies.
Conclusion
The lifespan prolonging effects of polydatin and its ameliorative role on PbAc-mediated toxicity in the flies may be due to its improvement in the behavioural deficits, antioxidant and anti-inflammatory properties.
Quarantine and antibiotic susceptibility of Enterobacteriaceae strains and other gram negative bacteria in dairy sweetmeat milk (doodh) peda
Authors: Amreen Fouzia , Puttananjaiaha Mohankumari Honganoor , Viswanath Prema 
Published: September 2022

Abstract
The aim of the present study was to assess the prevalence and isolation of Enterobacteriaceae strains and other gram negative bacteria of milk (doodh) peda-a traditional milk product of India. Bacteria of the family Enterobacteriaceae are the indicator organisms used to provide evidence of poor hygiene, inadequate processing or post-process contamination of foods. A total of 25 peda samples were collected from different places of Mysuru district to evaluate the prevalence of Enterobacteriaceae. Seventeen Gram negative fermentative bacteria of clinical significance were detected, isolated and subsequently identified using API 20E strips method and other associated biochemical tests. Our outcomes indicate that milk peda were contaminated with the Enterobacteriaceae like Enterobacter cloacae, Yersinia bercovieri, Yersinia rohdei, Raoutlella terigena, Acinetobacter lwoffii, Pantoea agglomerans PA2, Klebsiella pneumoniae subsp pneumoniae, Leclericia adecarboxylata, Photorhabdus nematophilus, Enterobacter gergoviae, Acinetobacter baumannii/calcoacetius, Cronobacter sakazaki (Enterobacter sakazaki). Further, the antimicrobial resistant of these Enterobacteriaceae group were also investigated against 20 antibiotics by the disc diffusion assay method. All isolates revealed susceptibility to the fluoroquinones and aminoglycosides, but resistance to the nitrofurantoin (70.59%), augmentin (52.94%), and cefpodoxime (47.06%) antibiotics. Our finding was the first report of prevalence and detection of E. gergoviae in a food sample (milk peda) and examined for antibiotic resistances.
Research Advances in Argonaute Proteins
Authors: Wei jianming , Zhang Chaoyue , Gao Bin , Zheng Yin , Feng Wenzhuo , Crabbe M. James C. , Li Yunzhou , Zhang Dalong 
Published: September 2022

Abstract
Background: Argonaute (AGO) is a large family of proteins and a primary component of the RNA-induced silencing complex (RISC). AGO proteins play important roles in regulatory processes, including RNA interference (RNAi) and gene silencing involving many small RNAs. RISC recognizes RNA by base complementary pairing and cleaves or suppresses translation to silence related genes. AGO proteins contain two domains: P-element induced wimpy testis (PIWI) and PIWI-Argonaute-Zwille, which can interact with small interfering RNA or target mRNA and play a key role in RNAi. The AGO protein family is also involved in biological defense mechanisms and may even become a new genome editing tool.
Methods and Results: This review collates and summarizes relevant literature in AGO protein research and describes the biological structure, functions, and characteristics of AGO proteins.
Conclusions: This review paper will provide a reference for related research and applications.
CircPARD3 promotes osteosarcoma proliferation and metastasis through regulating the miRNA-1294/ SKIL Pathway
Authors: Lv Guohua , Pan Yue , Wang Xiaoxiao , Li Yunchao , Li Xingyi 
Published: September 2022

Abstract
Background: Osteosarcoma (OS) is the most common malignant bone tumor in the pediatric population. The main goal of this study is to investigate the role of circPARD3 and the underlying signaling pathway involved in OS.Methods: Cell proliferation was measured using a CCK-8 assay kit and clone formation assay. Cell migration and invasion was measured using transwell assay kit and wound-healing assay. Change of RNA and protein expression was determined using RNA extract and quantitative real time PCR (RT-qPCR) assay and Western blotting, respectively. RNA immune precipitation and Luciferase assay was used to confirm the predicted results. The xenograft model was established to evaluate the function of circPARD3 in vivo.Results: Expression of circPARD3 was upregulated in both OS tissues and cell lines. Silencing circPARD3 repressed OS cell proliferation and metastasis, while overexpression of circPARD3 promote OS cell tumorigenesis in vitro. Furthermore, up-regulation of circPARD3 significantly promoted the growth of OS cells in vivo. Moreover, circPARD3 directly and negatively modulated the expression of miR-1294 and positively regulated the expression of SKIL. Conclusion: Abnormally high expression of circPARD3 may promote the proliferation, migration, and invasion of OS cells through up-regulating SKIL by sponging miR-1294. These results provide insight into therapeutic targets for preventing and treating OS.
Conservation Priorities for the Genus Serjania (Sapindaceae, Paullinieae), a Key Component in the Secondary Succesion of the Seasonally Dry Tropical Forest in Bolivia
Authors: Coulleri Juan P. , Brem Marta C. 
Published: September 2022

AbstractBolivia is a country with a huge environmental variety, most of which are classified as seasonally dry forest, these ecosystems are threatened due land use, then subsequent abandonment promote the regrowth of the forest by secondary succession. Therefore, identify species that lead this process is key in order to protect them, being lianas good candidates as it theSerjaniagenus. Our objectives were defining conservation priorities ofSerjaniaspecies richness estimation; determine environmental factors that influence the distribution of these species and understand the dynamic of the SDTFs and secondary forest in America through present and futureSerjanianiche modelling. Our results set the conservation priorities in the Yungas slopes and the Chiquitano dry forest of Bolivia, these are the most diverse forests formations of the country, being the Chiquitano dry forest one of the most long-term stable American dry forests. Regarding to the environmental factors, for the species that only vegetate in the Chiquitano dry forest the temperature was the most important one, while the species that inhabit in the Yungas slope the precipitation regime represent the most influential variable for the presence of the species in these habitats. As for the dynamics of the Seasonally dry forest throughSerjaniadistribution modelling, an increase in the land coverage by these ones at the expense of their neighboring wet forest is projected, which also indicate the progression of the secondary forest due land use and climate change.
Structural variation of GL1 gene determines the trichome formation in Brassica juncea
Authors: Meng Yiqing , Lyu Xiaolong , Liu Jiaqi , Gao Wei , Ma Yuyuan , Liao Nanqiao , Li Zhangping , Bo Yongming , Hu Zhongyuan , Yang Jinghua , Zhang Mingfang 
Published: September 2022

Abstract
Mustards (Brassica juncea) are allopolyploid crops in the worldwide, and trichomes are essential quality attributes that significantly influence its taste and palpability in vegetable-use cultivars. As important accessory tissues from specialized epidermal cells, trichomes also play an important role in mitigating biotic and abiotic stresses. In this study, we constructed a F2 segregating population using YJ27 with intensive trichome leaves and 03B0307 with glabrous leaves as parents. By bulked segregant analysis (BSA-seq), we obtained a 2.1 Mb candidate region on B02 chromosome associated with the trichome or glabrous trait formation. Then we used 13 Kompetitive Allele Specific PCR (KASP) markers for fine mapping and finally narrowed down the candidate region to about 448 kb in length. Interestingly, among the region, there was a 3 kb sequence deletion that located on the BjuVB02G54610gene in the F2 individuals with trichome leaves. Genotyping results of F2 populations confirmed this deletion (R2=81.44%) as a major QTL. Natural population resequencing analysis and genotyping results further validated the key role of the 3 kb structure variation (SV) of insertion/deletion type in trichome development in B. juncea. Our findings provide important information on the formation of trichomes and potential target gene for breeding vegetable mustards.
Sequential Band-Ratio in Landsat 8 OLI/TIRS to Enhance the Suspended Sediment Direction Monitoring in Urban Coastal Area
Authors: Trihatmoko Edy , Sartohadi Junun , Marfai Muh Aris , Hizbaron Dyah Rahmawati , Juhadi Juhadi , Pratiwi Elok Surya , Purwitaningsih Santika , Wongsokarto Misdianto 
Published: September 2022

Abstract
Various environmental processes as impacts of urban areas related to coastal dynamics require continuous monitoring using the Geographic Information System (GIS). Among such consequences, the spread pattern of suspended sediment in urban coastal areas indicates urban flood risk. This research aimed to determine the spread pattern of suspended sediment in the urban coastal area of Kendal Regency, Indonesia. The primary research method employed ENVI to analyze the sequential band-ratio from Landsat 7 TM to Landsat 8 OLI/TIRS satellite images. The results show that the distribution patterns effectively use band 3/2 of Landsat 8 OLI/TIRS with ca. 0.1 higher for the DN value compared to the band 4/3. This finding indicates different cases in other regions that mostly enhance the band ratio of 4/3 – sequentially match with band 3/2 in Landsat 7 TM. By receiving clear visualization from a band ratio of 3/2, the potential major distribution of suspended sediment was seen in the northeast during the west-east transitional season. However, the East-West's transitional season shows the sediment's circulation at certain points that could become the potential benchmark locations for the siltation occurrence because of sediment concentrations.
The consumption of date palm fruits as a source of bioactive compounds in patients with type 2 diabetes: a cross sectional study
Authors: Al-Mssallem Muneera 
Published: September 2022

Abstract
It is commonly supposed among the public and health care providers that patients with diabetes should restrain or even avoid consumption of date palm fruits (DPFs). However, DPFs are a good source of nutritive and non-nutritive bioactive compounds. This study aimed to assess the nutritive and non-nutritive bioactive compounds of consumed DPFs and examine their relationship with glycaemic control in patients with type 2 diabetes mellitus (T2DM) currently using oral antidiabetic medications (n=199) and insulin treatments (n=205). Blood glucose levels, HbA1c, total cholesterol, high-density lipoprotein, low-density lipoprotein, and triglycerides were retrieved from the medical records of the patients. The bioactive compound indices of consumed DPFs were calculated. The results revealed that the consumption of DPFs was higher in patients with oral medications than in those on insulin treatments. The bioactive compound content of the consumed DPFs ranged from 4.80±0.18 g/d for non-starch polysaccharides (NSPs) to 178±6.83 mg/d for selenium. However, the selenium index (273%) was the highest bioactive compound index in the consumed DPFs. Moreover, this study has detected a weak but significant correlation between the level of bioactive compounds of consumed DPFs and HbA1c levels. The study has revealed that the bioactive compound content of DPFs is negatively associated with lower HbA1c levels among patients with T2DM. An intervention trial is required to support this finding.