bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the Eco-environmental Quality Index in the Tropical Rainforest National Park in China during 1990-2020
Authors: Jian Kai , Wang Shengnan , Wu Xiaoqi , Zhang Qing 
Published: September 2022

Abstract
To precisely clarify the eco-environment quality status of the Tropical Rainforest National Park, this study develops and validates an eco-environmental quality index (EQI) model applicable to the evaluation of the eco-environment quality of the Tropical Rainforest National Park by using the Google Earth Engine (GEE) as a platform, based on the RSEI model, and applying standardized processing of indicators, artificial correction of PCA eigenvectors, and stepwise regression analysis modeling. And the EQI change trends, change processes, and spatial distribution of significant changes in the Tropical Rainforest National Park are analyzed at two different levels, namely dynamic changes in ecological environmental quality and spatial identification of time series changes, respectively. We also investigate the effects of factors such as the model's internal indicators, topographical elements, and the distribution of land use cover types on the changes in EQI. The results indicate that: 1) The application of the EQI4 model could invert the EQI spatial distribution map consistent with the RSEI model, and the overall change of EQI in tropical rainforest national parks estimates using the EQI4 model reveals a stable and slightly decreasing trend with obvious process volatility. 2) The Tropical Rainforest National Park's EQI spatial distribution and fluctuation zoning has shown obvious spatial heterogeneity. The proportion of spatial area with significant changes in EQI increases as the study time series progress, and the deterioration trend of its time series EQI is the most pronounced in the two regions represented by Jianfengling and Diaoluoshan. (3) The distribution of EQI coefficients of variation in Tropical Rainforest National Park is in excellent agreement with those of NDVI and WET, as measured by R2 values of 0.69 and 0.79, respectively. The bivariate clustering of EQI coefficients of variation with topographic elements reveals that low-elevation, gently sloping areas with frequent human activities, and forest cash crop cultivation areas have greater EQI fluctuations. Consequently, timely, accurate, and rapid quantitative assessment and spatial and temporal change analysis of the eco-environmental quality of Tropical Rainforest National Park can promote the protection of natural resources and ecologically sustainable development of the national park.
Towards Automatic Text-Based Estimation of Depression through Symptom Prediction
Authors: Milintsevich Kirill , Sirts Kairit , Dias Gaël 
Published: September 2022

Abstract
Major Depressive Disorder (MDD) is one of the most common and comorbid mental disorders that impacts a person's day-to-day activity. Additionally, MDD affects one's linguistic footprint, which is reflected by subtle changes in speech production. This allows us to use natural language processing (NLP) techniques to build a neural classifier to detect depression from speech transcripts. MDD is often assessed with a self-reported questionnaire; the final diagnosis is made by summing up question scores and checking them against a predefined cut-off point. This approach, however, disregards the complexity of the clinical picture of depression, as different people with MDD can suffer from different sets of depression symptoms for the same self-reported depression severity score. In this work, we look at the depression classification problem through the prism of the symptom network analysis approach, which shifts attention from categorical analysis of depression towards personalized analysis of symptom profiles. For that purpose, we trained a multi-target hierarchical regression model to predict individual depression symptoms from patient-psychiatrist interview transcripts from the DAIC-WOZ corpus. Our model achieved results on par with state-of-the-art models on both binary diagnostic classification, and depression severity prediction while at the same time providing a more fine-grained overview of individual symptoms for each person. The model achieved a mean absolute error (MAE) from 0.438 to 0.830 on eight depression symptoms and showed state-of-the-art results in binary depression estimation (73.9 macro-F1) and total depression score prediction (3.78 MAE). Moreover, the model produced a symptom correlation graph that is structurally identical to the real one. The proposed symptom-based approach provides more in-depth information about the depressive condition by focusing on the individual symptoms rather than a general binary diagnosis.
Transplantation of A2 Type Astrocytes Promotes Neural Repair and Remyelination After Spinal Cord Injury
Authors: Chang Jie , Qian Zhanyang , Wang Binyu , Cao Jiang , Zhang Sheng , Jiang Fan , Kong Renyi , Yu Xiao , Cao Xiaojian , Yang Lei , Chen Hongtao 
Published: September 2022

Abstract
Background
Limited progress in terms of an effective treatment for spinal cord injury (SCI) emphasizes the urgent need for novel therapies. As a vital central nervous system component, the resident astrocytes play crucial roles in regulating recovery after SCI. In this study, recovery after SCI was compared following the transplantation of either A1 or A2 astrocytes. A1 astrocytes are harmful as they upregulate the neurotoxic classical complement cascade genes. Conversely, A2 astrocytes are characterized as neuroprotective as they upregulate the production of many neurotrophic factors.
Methods
We used different supernatant obtained from microglia stimulated with lipopolysaccharide or interleukin-4 to generate A1 and A2 astrocytes. We detected the influence of astrocytes on neurons by co-culturing A1 and A2 astrocytes with neurons. We transplanted astrocytes into the lesion site of the spinal cord and assessed lesion progression, neural restoration, glia formation and locomotor recovery.
Results
Astrocytes were polarized into A1 and A2 phenotypes following culture in the supernatant obtained from microglia stimulated with lipopolysaccharide or interleukin-4, respectively. Furthermore, co-culturing A2 astrocytes with neurons significantly suppressed glutamate-induced neuronal apoptosis and promoted the degree of neuron arborization. Transplantation of these A2 astrocytes into the lesion site of the spinal cord of mice significantly improved motor function recovery, preserved spared supraspinal pathways, decreased glia scar deposition, and increased neurofilament formation at the site of injury compared to the transplantation of A1 astrocytes. Additionally, enhanced A2 astrocytes with potentially beneficial A2-like genes were also detected in the A2 group. Moreover, luxol fast blue staining and electron microscopy indicated increased preservation of myelin with organized structure after transplantation of A2 astrocytes than of A1 astrocytes.
Conclusions
A2 astrocyte transplantation could be a promising potential therapy for SCI.
Classification on Boundary-equilibria and Singular Continuums of Continuous Piecewise Linear Systems
Authors: Chen Hebai , Feng Zhaosheng , Yang Hao , Zhou Linfeng 
Published: September 2022

Abstract
In this paper, we show that any switching hypersurface of n-dimensional continuous piecewise linear systems is an (n − 1)-dimensional hyperplane. For two-dimensional continuous piecewise linear systems, we present local phase portraits and indices near the boundary equilibria (i.e., equilibria at the switching line) and singular continuum (i.e., continuum of non-isolated equilibria) between two parallel switching lines. The definition of the index of singular continuum is introduced. Then we show that boundary-equilibria and singular continuums can appear with many parallel switching lines.
2010 Mathematics Subject Classification. Primary 34C05; 34A26; 49J52.
5-Aminolevulinic Acid Tumor Paint and Photodynamic Therapy for Chordoma: An In Vitro Study
Authors: Kenan Shachar , Nixon Ryan , Liang Haixiang , Goodman Howard J. , Grande Daniel A. , Levin Adam S. 
Published: September 2022

Abstract
Background:
Chordomas are malignant tumors originating from embryonic notochord remnants. Wide resection is challenging due to lack of intraoperative visualization, leading to unacceptably high recurrence rates. Known techniques using five-aminolevulinic acid (5-ALA) for tumor paint and photodynamic therapy (PDT) may improve outcomes but have not been well described for chordomas. The purpose of this study was to analyze chordoma cell response to 5-ALA tumor paint and PDT in vitro.
Methods:
Tumor Paint: Experiment 1: Human chordoma cells (MUG-Chor1) co-cultured with green fluorescence protein (GFP) rat adipose derived stromal cells (ADS) were observed 5 hours after 5-ALA exposure using emission filters showing GFP cells as green and 5-ALA positive cells as red. Experiment 2: GFP-ADS and MUG-Chor1 micromass pellets were co-cultured and observed 5 hours after 5-ALA exposure. Experiment 3: co-cultured human ADS and MUG-Chor1 cells were observed 5 hours after 5-ALA exposure.
Photodynamic Therapy: MUG-Chor1 cells that had been exposed to 3 hours of 5-ALA were visualized at 10× with 405-nm excitation and emission at 603-738-nm. Time-lapse images of live cells were captured every second for 15 minutes and then visualized sequentially at 50× speed.
Results:
Tumor Paint: In the first experiment, chordoma cells displayed minimal red fluorescence while GFP-ADS cells appeared to fluoresce expectedly green. In the second experiment, chordoma cells fluoresced brightly red. As a micromass, the distinction between chordoma cells and GFP-ADS cells was clearly seen. The third experiment showed strong red fluorescence in high cellular density areas, yet minimal fluorescence in low cellular density areas.
PDT: Initially, chordoma cells fluoresced red in response to the 405-nm excitation laser exposure. As time progressed, increased intracellular swelling occurred with concomitant rapid formation of multiple vesicles exiting from the cellular membrane.
Conclusions:
The results of these experiments demonstrate the effective in vitro application of 5-ALA tumor paint and PDT on chordoma cells. This will be the first time this phenomenon is documented visually using time-lapse photography for this tumor subtype, further validating prior research in this field. 5-ALA, with its double-edged applications of selective tumor identification and kill may lead to transformative change in the management of soft tissue sarcomas.
Monitoring phage-induced lysis of Gram-negatives in real time using a fluorescent DNA dye
Authors: Egido Julia E. , Toner-Bartelds Catherine , Costa Ana Rita , Brouns Stan J. J. , Rooijakkers Suzan H. M. , Bardoel Bart W. , Haas Pieter-Jan 
Published: September 2022

Abstract
Bacteriophages (phages) are viruses that specifically attack bacteria. Their use as therapeutics, which constitutes a promising alternative to antibiotics, heavily relies on selecting effective lytic phages against the pathogen of interest. Current selection techniques are laborious and do not allow for direct visualization of phage infection dynamics. Here, we present a method that circumvents these limitations. It can be scaled for high-throughput and permits monitoring of the phage infection in real time via a fluorescence signal readout. This is achieved through the use of a membrane-impermeant nucleic acid dye that stains the DNA of damaged or lysed bacteria and new phage progeny. We have tested the method on Pseudomonas aeruginosa and Klebsiella pneumoniae and show that an increase in fluorescence reflects phage-mediated killing. This is confirmed by other techniques including spot tests, colony plating, flow cytometry and metabolic activity measurements. Furthermore, we illustrate how our method may be used to compare the activity of different phages and to screen the susceptibility of clinical isolates to phage. Altogether, we present a fast, reliable way of selecting phages against Gram-negative bacteria, which may be valuable in optimizing the process of selecting phages for therapeutic use.
WITHDRAWN: Technological Profile of Small-diameter Forest Species in a Managed Area in the Amazon
Authors: Nascimento Cristiano Souza , Cruz Irineide Almeida , Nascimento Claudete Catanhede , Araújo Roberto Daniel , Higuchi Niro , Santos Joaquim dos 
Published: September 2022

Abstract

The use of wood from small-diameter species is still restricted, and knowledge of its characteristics is limited. In this context, the objective of this study was to characterize the technological properties of eight species of small diameters of greater occurrence in the Central Amazon to indicate sustainable use. Samples were obtained from a managed area of secondary forest (Amazonas/Brazil). 24 trees (diameter ≤ 50 cm) were selected for the determination of chemical and physicomechanical properties. The highest concentrations of extractives and total polyphenols were detected for Eschweilera odora (7.08 and 2.63%), and lignin and cellulose were detected for Micrandropsis scleroxylon (34.80%) and Byrsonima crispa (55.62%). For the physical-mechanical properties, the average moisture content was 12.84%. For density, the species were classified in the medium to high range (0.56-0.93 g/cm3). In general, the studied species presented a high calorific value (~4,907 cal/g), and Eschweilera truncata presented higher mechanical strength (modulus of elasticity 17,350 MPa; modulus of rupture 173.93 MPa). The multivariate analysis using the K-means algorithm, based on the centroid of the data, indicated the formation of five groups, where the group of Eschweilera truncata was represented by higher values of MOE, MOR, and ash, while the group of Inga alba had characteristics of low-strength wood. The quality of the small-diameter wood studied here has the potential to be indicated for management since the technological characterization is a fundamental tool to assist decision-making in management plans that may indicate the use of new species in the forestry sector.
Partial Replacement Imputation Estimation for Partially Linear Models with Complex Missing Pattern Covariates
Authors: Zhan Zishu , Li Xiangjie , Zhang Jingxiao 
Published: September 2022

Abstract
Missing data is a common problem in clinical data collection, which causes difficulty in the statistical analysis of such data. In this article, we consider the problem under a framework of a semiparametric partially linear model when observations are subject to complex missing pattern covariates. One natural question in the partially linear model is the choice of model structure, that is, how to decide which covariates are linear and which are nonlinear. If the correct model structure of the partially linear model is available, we propose to use a new imputation method called Partial Replacement IMputation Estimation (PRIME), which can overcome problems caused by incomplete data in the partially linear model. In the more challenging setting where the model structure is unknown a priori, we use PRIME in conjunction with model averaging (PRIME-MA) to adapt to the unknown model structure in the partially linear model. In simulation studies, we use various error distributions, sample sizes, missing data rates, covariate correlations, and noise levels, and PRIME outperforms other methods in almost all cases. With an unknown correct model structure, PRIME-MA has satisfactory performance in terms of prediction. Moreover, we conduct a study of influential factors in the Chinese Provincial Legal Funding Dataset from the Harvard Dataverse, which shows that our method performs better than the other models.
Effects of 6 weeks of low-volume combined training on muscle power, muscular strength, and aerobic power in active young adults.
Authors: Martins Ricardo Jorge Costa , Loureiro Nuno Eduardo Marques 
Published: September 2022

Abstract
Background: The aim of this study was to analyse the effects of low-volume CT performed during 6 weeks on muscle power, muscular strength, maximal aerobic power (Wmax) and internal load in active young adults.
Methods: Eighteen healthy, active young adults men (mean ± SD, 20.06 ± 1.66 years; 22.23 ± 2.76 kg-1m2) performed either a low-volume CT (GE, n=9), or maintained a normal life (CG, n=9). The CT was composed of a resistance training (RT, 2 sets of 3 exercises with 80 to 85% 1RM) followed by a high intensity-interval training (HIIT, 5 sets of 60’’ with 95% Wmax). The measures of jump height, 1 maximal repetition (1RM) in bench press and back squat, Wmax and internal load were obtained before (pre) and after (post) training to analysis. Furthermore, an ANOVA test of repeated measures and t-test paired samples were used with a p ≤ 0.05.
Results: Low-volume CT increased from pre to post on jump height (29.28 ± 3.81 to 32.02 ± 3.09cm, p ≤ 0.05), 1RM on bench press back squat (56.11 ± 11.35 to 67.67 ± 13.36kg, p < 0.001 and 63.11 ± 12.25 to 74.00 ± 12.02kg, p < 0.001, respectively) and Wmax (200 ± 30 to 220 ± 30.92W, p ≤ 0.01). The internal load had not significant differences between weeks (p > 0.05).
Conclusions: In healthy, active young adults men the low-volume CT is effective to improve, jump height, 1RM in bench press and back squat, and Wmax without increase internal load.
A dark side of conservation biology: protected areas fail in representing subterranean biodiversity
Authors: Colado Raquel , Abellán Pedro , Pallarés Susana , Mammola Stefano , Milione Roberto , Faille Arnaud , Fresneda Javier , Fernández David Sánchez 
Published: September 2022

Abstract
The conservation of biodiversity is a central imperative of the 21st century. Subterranean ecosystems deliver critical nature’s contributions to people and harbour a broad diversity of poorly-understood specialized organisms that are of interest from both a conservation and evolutionary perspective. However, the subterranean biome is still systematically overlooked in global biodiversity targets and conservation agendas. The main objective of this study was to assess how far subterranean biodiversity is represented in protected areas (Natura 2000 and Emerald networks) in two global hotspots of subterranean biodiversity (the Pyrenees and the Alps). For this, we used the most complete databases of terrestrial subterranean biodiversity known to us, i.e., leiodids (beetles) from the Pyrenees and spiders from the Alps, and identified priority areas in each region using both species richness and geographic rarity patterns. Our results show the incapacity of surface protected area networks to represent subterranean fauna, as more than 70 and 90% of the identified priority areas (and the 40 and 22% of the species) are not effectively covered by protected areas in the Pyrenees and the Alps, respectively. These findings call for urgent policies and would be key to developing a coherent plan for subterranean biodiversity conservation within the European Biodiversity Strategy for 2030.
Older adults access to mental health and social care services during COVID-19 restrictions in Western Australia
Authors: Adams Claire , Gringart Eyal , McAullay Daniel , Sim Moira , Scarfe Brigitta , Budrikis Amy , Strobel Natalie 
Published: September 2022

Abstract
Objective: This study aimed to understand the impact of COVID-19 on access to mental health and social services among older adults in Western Australia.
Method: A cross-sectional study was conducted with 194 adults aged ≥70 years or ≥60 years with chronic conditions. A questionnaire co-developed by a consumer reference group was used to collect data on social networks and service access. Frequency analyses were used to assess quantitative data. Qualitative data were assessed using thematic analyses.
Results: 62.7% of participants reported being not at all/slightly affected by COVID-19; 40.7% reported having three/four people to chat with. 76.3% of participants did not access mental health or social services during the 2020 COVID-19 restrictions. The remaining 23.7% mostly accessed mental health-related services, with GPs the most common source of support. 18.0% of the total sample reported choosing not to access services even though they would have liked to.
Conclusions: Most older adults in this sample did not access mental health or social care services. 18.0% of all participants felt they needed services but did not access them. This suggests there were some unmet needs within the community. Strengthening social networks may help protect older adults against psychosocial declines during and post-COVID-19.
In Short, In Hort
Authors: Jefferson Thomas 
Published: September 2022

Abstract
FOOD GLEANING FEEDS PORTLAND’S POOR
RELEASE OF DWARF MUGO PINE SELECTIONS
AG COLLEGE ENROLLMENTS SOAR
SQUANTO – AMERICA’S FIRST EXTENSION HORTICULTURIST
AG RESEARCH EATS LOW ON THE HOG
FARM POPULATION, UP IN WEST, DOWN ELSEWHERE
ROOT ZONE HEATING SAVES FUEL
PRODUCTION OF CHLORDIMEFORMS HALTED
PESTICIDE USAGE FOR UNNAMED PESTS
‘CONCORD’ GRAPES WEAKENED BY OZONE
MEAN BEAN STABS LEAFHOPPERS
OH YE HYPOCRITES!
ORNAMENTALS BIG BUCKS IN L.A. COUNTY
LEAD IN PLANTS AND ANIMALS INHABITING OLD ORCHARD SOILS
International
The Maintenance of Professional Integrity in the Interdisciplinary Team Research Effort
Authors: Mitchell Roger L. 
Published: September 2022

Abstract
The identity and integrity of our professional disciplines is of special importance. We believe those disciplines make unique contributions to the intellectual environment of the university, research laboratory, or field station in addition to the problem solving expected of us by the citizens of our several states, the nation, and indeed the world community. Each of us has invested major portions of our lives toward building and maintaining a foundation and a framework for specialization and uniqueness. We find it necessary to keep others informed about the intellectual areas on which we are well qualified to serve. And while we have not resorted to unionization to maintain our positions we do expend significant energy in committee meetings, curricular review, degree program building, promotion and tenure review processes, and professional society activities to ensure quality performance and preparation by our peers as well as to maintain our own professional integrity.
Promotion of Rooting in Stem Cuttings of Several Ornamental Plants by Pretreatment with Acid or Base1
Authors: Lee C. I. , Paul J. L. , Hackett W. P. 
Published: September 2022

Abstract
Rooting of stem cuttings of Bougainvillea cv. San Diego Red, Ceratonia siliqua L., Chrysanthemum morifolium Ramat. cvs. Golden Anne and Mandalay, Euonymus japonica L. cv. Yellow Edge, Euphorbia pulcherrima Willd. cv. Eckspoint C-1 Red, Hedera helix L., Trachelospermum jasminoides [Lindl.] Lem., Juglans hindsii (Jeps.) Jeps., Pistacia chinensis Bunge, and Salix laevigata Bebb. is greatly promoted by basal dipping in H2SO4 prior to applying indolebutryic acid. Pre-treatment with NaOH results in considerable increase of rooting of cuttings of Rhododendron (Pericat) cv. Sweetheart Supreme, Bougainvillea, Liquidambar styraciflua L., Osmanthus heterophyllus G. Don cv. Ilicifolius, and Pinus radiata D. Don.
Germination and Container Production of Franklinia1
Authors: Farmer R. E. , Chase S. B. 
Published: September 2022

Abstract
Unstratified seed of Franklinia alatamaha Marsh germinated slowly in light at 16° to 29°C. During stratification at 3° for 4 to 12 weeks, seed acquired the capability for germination in the dark and at lower temperatures. Data on germination percent and speed suggested that 4 to 8 weeks' stratification followed by germination at relatively high temperatures should result in good stands within 20 days. A greenhouse production system was developed using this information, and plants were grown to salable size in marketing containers.
In Vitro Culture of Immature Embryos of American Elm1
Authors: Anagnostakis S. L. 
Published: September 2022

Abstract
Immature 2n embryos of Ulmus americana L. were excised aseptically from seeds to 20 different media. A low salts medium with coconut water allowed the largest number of embryos to become green and begin stem elongation and true leaf formation. None of the resulting plants survived transplanting.