bluuebunny/crossref_metadata_2025_split huggingface dataset:
Views of Hospital Nurses and Nursing Students on Nursing Engagement—Bridging the Gap Through Communication Courses
Authors: Huang Qing , Pun Jack 
Published: June 2022

BackgroundCommunicative engagement plays a significant role for effective nurse–patient communication. In the existing college nursing communication training within and outside China, there is a difference between what students are taught and what they can apply in their clinical placements.AimsUsing needs analysis, this mixed-methods study explored potential gaps between frontline hospital nurses' and college nursing students' perceptions of nurse–patient communicative engagement, and collated a list of effective engagement strategies for pedagogy.MethodsSurveys and interviews were conducted with key stakeholders, including 16 hospital nurses and 60 nursing students. A new scale named Nursing Engagement with Patients Scale (NEPS) was developed and validated to explore stakeholders' views on nursing engagement.ResultsDifferences between the views of nurses and students on engagement were identified. While frontline nurses affirmed the importance of engaging with patients while providing nursing care, nursing students were unsure about the concept and role of engagement, and how to enact it. A list of communication strategies that promote engagement was culled from the interviews with the experienced nurses.ImplicationsThese can be used to inform nursing communication courses to bridge the gap between what nursing students are currently taught and what they will need in the workplace.
COVID-19 Pandemic: The Impact of COVID-19 on Mental Health and Life Habits in the Canadian Population
Authors: Iftene Felicia , Milev Roumen , Farcas Adriana , Squires Scott , Smirnova Daria , Fountoulakis Konstantinos N. 
Published: June 2022

ObjectivesThe study aims to investigate the rate of clinical depression in the adult population during the COVID-19 pandemic, as well as the changes in anxiety, distress, suicidal ideation, and their relations with several personal and interpersonal/social variables.MethodsThis is an epidemiological, non-interventional study. It is part of an international multi-center study, with the main site at the Aristotle University of Thessaloniki, in Greece (COMET-G Study). We are presenting aspects of the research involving the Canadian site, based on 508 Canadian responders to the online survey (QAIRE).ResultsOf the 508 responders, 72.2% were females aged 42.57 ± 14.00 years; 27.2% were males aged 42.24 ± 15.49 years; and 0.6% were others aged 46.33 ± 17.79 years. Increased anxiety during the lockdown was reported by 69.3% of those surveyed. The rate of suicidal thoughts increased in 19.5% of participants during the lockdown. Depression was reported by 22% of responders, while distress was present in 18.4%. We found a greater prevalence of depression, but not distress, in individuals with a history of any mental disorder. Based on the multiple regression analysis, we found four CORE factors equally influencing the changes in mental health during the lockdown (gender, quality of sleep, family conflicts, and changes in daily routine). In the Canadian population, two major changes acted as protective factors, significantly expressed when compared with the worldwide tendencies: fewer financial difficulties; and an increase in religious beliefs.ConclusionThe rate of major depression, distress, and suicidal ideation was higher in Canadians than in the worldwide population (per COMET-G), but the relative risk to develop depression in the presence of a history of mental disorders was lower. Almost 90% of Canadians believed in the real story of COVID source of provenience.
Prevalence and Correlates of Mental Health of University Students in Hong Kong: What Happened One Year After the Occurrence of COVID-19?
Authors: Shek Daniel T. L. , Dou Diya , Zhu Xiaoqin 
Published: June 2022

PurposeIn this study, we studied the prevalence and correlates of mental health problems (depression, anxiety, and stress) in Hong Kong university students after one year of the occurrence of COVID-19. We examined the relationships between mental health problems and socio-demographic factors (including age, gender, local/international students, living status, and economic strain), need satisfaction, and difficulties encountered.MethodsA cross-sectional online survey involving 1,648 university students (Mean age = 20.09 years ± 1.37) was conducted. They responded to a questionnaire assessing their mental health (including the Depression, Anxiety, and Stress Scales—DASS), psychosocial resources, social support, need satisfaction, difficulties and challenges, and evaluation of services they received.ResultsBased on DASS cutoff scores, prevalence rates of moderate or above levels of depression, anxiety, and stress were 40.0, 50.7, and 22.2%, respectively. While age and gender were not related to the DASS measures, economic strain and living alone were positively related to negative emotional states based on the results of MANOVAs. Multiple regression and PROCESS analyses showed that need satisfaction and difficulties contributed to depression and anxiety, with stress as a mediating factor.ConclusionsThe proportions of students who experienced moderate and above levels of depression (40.0%), anxiety (50.7%), and stress (22.2%) indexed by DASS deserve attention. Living alone and experienced economic disadvantage are risk factors for negative emotional states. Need satisfaction and life challenges are related to negative emotional states. Need satisfaction and difficulties contributed to stress, which further influenced anxiety and depression.
Telephone Triage for Emergency Patients Reduces Unnecessary Ambulance Use: A Propensity Score Analysis With Population-Based Data in Osaka City, Japan
Authors: Katayama Yusuke , Kitamura Tetsuhisa , Nakao Shunichiro , Himura Hoshi , Deguchi Ryo , Tai Shunsuke , Tsujino Junya , Mizobata Yasumitsu , Shimazu Takeshi , Nakagawa Yuko 
Published: June 2022

BackgroundTelephone triage service in emergency care has been introduced around the world, but the impact of this service on the emergency medical service (EMS) system has not been fully revealed. The aim of this study was to evaluate the effect of telephone triage service for emergency patients on decreasing unnecessary ambulance use by analysis with propensity score (PS) matching.MethodsThis study was a retrospective observational study, and the study period was the 4 years from January 2016 to December 2019. We included cases for which ambulances were dispatched from the Osaka Municipal Fire Department (OMFD). The primary outcome of this study was unnecessary ambulance use. We calculated a PS by fitting a logistic regression model to adjust for 10 variables that existed before use of the telephone triage service. To ensure the robustness of this analysis, we used not only PS matching but also a multivariable logistic regression model and regression model with PS as a covariate.ResultsThis study included 868,548 cases, of which 8,828 (1.0%) used telephone triage services and 859,720 (99.0%) did not use this service. Use of the telephone triage service was inversely associated with the occurrence of unnecessary ambulance use in multivariate logistic regression model (adjusted OR 0.453, 95% CI 0.405–0.506) and multivariate logistic regression model with PS as a covariate (adjusted OR 0.514, 95% CI 0.460–0.574). In the PS matching model, we also revealed same results (crude OR 0.487, 95% CI 0.425–0.588).ConclusionsIn this study, we were able to statistically evaluate the effectiveness of telephone triage service already in use by the public using the statistical method with PS. As a result, it was revealed that the use of a telephone triage service was associated with a lower proportion of unnecessary ambulance use in a metropolitan area of Japan.
Combination of Autophagy Selective Therapeutics With Doxil: An Assessment of Pathological Toxicity
Authors: Helke Kristi L. , Gudi Radhika R. , Vasu Chenthamarakshan , Delaney Joe R. 
Published: June 2022

Background: Combination therapy of targeted drugs in cancer treatment is a field in constant flux, with research balancing side effects with efficacy. Efficacy from combination therapy is improved either through synthetic lethality or through prevention of recurrent clones. Previous research has shown (hydroxy-)chloroquine is insufficient to disrupt autophagy in tumors. Hence, either combinations or novel autophagy agents are desired. In vivo studies of ovarian cancer have revealed that chloroquine can be combined with up to four other autophagy drugs to suppress ovarian cancer growth. While cancer efficacy is now established for the autophagy drug combination, it is unclear what toxicities may require monitoring in human trials. Additive toxicity with chemotherapy is also unknown.Methods: To address toxicity in more depth than previous weight-monitoring studies, biochemical and histopathology studies were performed. Mouse groups were treated with autophagy drugs for 2 weeks, with or without the chemotherapy Doxil. After the last dose, mice were processed for blood biochemistry, white blood cell markers, and histopathology.Results: Data from a comprehensive blood biochemistry panel, flow cytometric measurements of blood cell markers, and histopathology are herein reported. While Doxil presented clear bone marrow and immunologic toxicity, autophagy drugs were overall less toxic and more variable in their presentation of potential toxicities. Only minor additive effects of autophagy drugs with Doxil were observed.Conclusion: Combinations of autophagy drugs may be considered for therapy in human oncology trials, with possible side effects to monitor informed by these murine pre-clinical data.
Recipient Comorbidities for Prediction of Primary Graft Dysfunction, Chronic Allograft Dysfunction and Survival After Lung Transplantation
Authors: Ehrsam Jonas Peter , Schuurmans Macé M. , Laager Mirjam , Opitz Isabelle , Inci Ilhan 
Published: June 2022

Since candidates with comorbidities are increasingly referred for lung transplantation, knowledge about comorbidities and their cumulative effect on outcomes is scarce. We retrospectively collected pretransplant comorbidities of all 513 adult recipients transplanted at our center between 1992–2019. Multiple logistic- and Cox regression models, adjusted for donor-, pre- and peri-operative variables, were used to detect independent risk factors for primary graft dysfunction grade-3 at 72 h (PGD3-T72), onset of chronic allograft dysfunction grade-3 (CLAD-3) and survival. An increasing comorbidity burden measured by Charleston-Deyo-Index was a multivariable risk for survival and PGD3-T72, but not for CLAD-3. Among comorbidities, congestive right heart failure or a mean pulmonary artery pressure &gt;25 mmHg were independent risk factors for PGD3-T72 and survival, and a borderline risk for CLAD-3. Left heart failure, chronic atrial fibrillation, arterial hypertension, moderate liver disease, peptic ulcer disease, gastroesophageal reflux, diabetes with end organ damage, moderate to severe renal disease, osteoporosis, and diverticulosis were also independent risk factors for survival. For PGD3-T72, a BMI&gt;30 kg/m2 was an additional independent risk. Epilepsy and a smoking history of the recipient of &gt;20packyears are additional independent risk factors for CLAD-3. The comorbidity profile should therefore be closely considered for further clinical decision making in candidate selection.
Insights into the Antimicrobial Activities and Metabolomes of Aquimarina (Flavobacteriaceae, Bacteroidetes) Species from the Rare Marine Biosphere
Authors: Silva Sandra Godinho , Paula Patrícia , da Silva José Paulo , Mil-Homens Dalila , Teixeira Miguel Cacho , Fialho Arsénio Mendes , Costa Rodrigo , Keller-Costa Tina 
Published: June 2022

Two novel natural products, the polyketide cuniculene and the peptide antibiotic aquimarin, were recently discovered from the marine bacterial genus Aquimarina. However, the diversity of the secondary metabolite biosynthetic gene clusters (SM-BGCs) in Aquimarina genomes indicates a far greater biosynthetic potential. In this study, nine representative Aquimarina strains were tested for antimicrobial activity against diverse human-pathogenic and marine microorganisms and subjected to metabolomic and genomic profiling. We found an inhibitory activity of most Aquimarina strains against Candida glabrata and marine Vibrio and Alphaproteobacteria species. Aquimarina sp. Aq135 and Aquimarina muelleri crude extracts showed particularly promising antimicrobial activities, amongst others against methicillin-resistant Staphylococcus aureus. The metabolomic and functional genomic profiles of Aquimarina spp. followed similar patterns and were shaped by phylogeny. SM-BGC and metabolomics networks suggest the presence of novel polyketides and peptides, including cyclic depsipeptide-related compounds. Moreover, exploration of the ‘Sponge Microbiome Project’ dataset revealed that Aquimarina spp. possess low-abundance distributions worldwide across multiple marine biotopes. Our study emphasizes the relevance of this member of the microbial rare biosphere as a promising source of novel natural products. We predict that future metabologenomics studies of Aquimarina species will expand the spectrum of known secondary metabolites and bioactivities from marine ecosystems.
Genetic and Morphological Diversity Assessment of Five Kalanchoe Genotypes by SCoT, ISSR and RAPD-PCR Markers
Authors: Al-Khayri Jameel M. , Mahdy Ehab M. B. , Taha Heba S. A. , Eldomiaty Ahmed S. , Abd-Elfattah Mohamed A. , Abdel Latef Arafat Abdel Hamed , Rezk Adel A. , Shehata Wael F. , Almaghasla Mustafa I. , Shalaby Tarek A. , Sattar Muhammad N. , Ghazzawy Hesham S. , Awad Mohamed F. , Alali Khalid M. , Jain Shri Mohan , Hassanin Abdallah A. 
Published: June 2022

Determining the appropriate parents for breeding programs is the most important decision that plant breeders must make to maximize the genetic variability and produce excellent recombinant genotypes. Several methods are used to identify genotypes with desirable phenotypic features for breeding experiments. In this study, five kalanchoe genotypes were morphologically characterized by assessing plant height, number of inflorescences, number of flowers, flower length, flower diameter and number of petals. The analysis showed the distinction of yellow kalanchoe in the plant height trait, while the orange kalanchoe was distinguished in the number of inflorescences, the number of flowers and flower length traits, whereas the violet kalanchoe possessed the largest flower diameter and the highest number of petals. The molecular profiling was performed by random amplified polymorphism DNA (RAPD), inter-simple sequence repeats (ISSR) and start codon targeted (SCoT)-polymerase chain reaction (PCR) tools. Genomic DNA was extracted from young leaves and the PCR reactions were performed using ten primers for each SCoT, ISSR and RAPD marker. Only four out of ten primers showed amplicon profiles in all PCR markers. A total of 70 bands were generated by SCoT, ISSR and RAPD-PCR with 35 polymorphic bands and 35 monomorphic bands. The total number of bands of RAPD, ISSR and SCoT was 15, 17 and 38, respectively. The polymorphism percentages achieved by RAPD, ISSR and SCoT were 60.25%, 15% and 57%, respectively. The cluster analysis based on morphological data revealed two clusters. Cluster I consisted of violet and orange kalanchoe, and cluster II comprised red, yellow and purple kalanchoe. Whereas the cluster analysis based on molecular data revealed three clusters. Cluster I included only yellow kalanchoe, cluster II comprised orange and violet kalanchoe while cluster III comprised red, and purple kalanchoe. The study concluded that orange, violet and yellow kalanchoe are distinguished parents for breeding economically valued traits in kalanchoe. Also, the study concluded that SCoT and RAPD markers reproduced reliable banding patterns to assess the genetic polymorphism among kalanchoe genotypes that consider the basis stone for genetic improvements in ornamental plants.
An Improved Submerged Mangrove Recognition Index-Based Method for Mapping Mangrove Forests by Removing the Disturbance of Tidal Dynamics and S. alterniflora
Authors: Xia Qing , He Ting-Ting , Qin Cheng-Zhi , Xing Xue-Min , Xiao Wu 
Published: June 2022

Currently, it is a great challenge for remote sensing technology to accurately map mangrove forests owing to periodic inundation. A submerged mangrove recognition index (SMRI) using two high- and low-tide images was recently proposed to remove the influence of tides and identify mangrove forests. However, when the tidal height of the selected low-tide image is not at the lowest tidal level, the corresponding SMRI does not function well, which results in mangrove forests below the low tidal height being undetected. Furthermore, Spartina alterniflora Loisel (S. alterniflora) was introduced to China in 1979 and rapidly spread to become the most serious invasive plant along the Chinese coastline. The current SMRI has failed to distinguish S. alterniflora from submerged mangrove forests because of their similar spectral signatures. In this study, an SMRI-based mangrove forest mapping method was developed using the time series of Sentinel-2 images to mitigate the two aforementioned issues. In the proposed method, quantile synthesis was applied to the time series of Sentinel-2 images to generate a lowest-tide synthetic image for creating SMRI to identify submerged mangrove forests. Unsubmerged mangrove forests were classified using a support vector machine, and a preliminary mangrove forest map was created by merging them. In addition, S. alterniflora was distinguished from the mangrove forests by analyzing their phenological differences. Finally, mangrove forest mapping was performed by masking S. alterniflora. The proposed method was applied to the entire coastline of the Guangxi Province, China. The results showed that it can reliably and accurately identify submerged mangrove forests derived from SMRI by synthesizing low- and high-tide images using quantile synthesis, and the differentiation of S. alterniflora using phenological differences results in more accurate mangrove mapping. This work helps to improve the accuracy of mangrove forest mapping using SMRI and its feasibility for coastal wetland monitoring. It also provides data for sustainable management, ecological protection, and restoration of vegetation in coastal zones.
Cadmium Caused Different Toxicity to Photosystem I and Photosystem II of Freshwater Unicellular Algae Chlorella pyrenoidosa (Chlorophyta)
Authors: Wang Shuzhi , Wufuer Rehemanjiang , Duo Jia , Li Wenfeng , Pan Xiangliang 
Published: June 2022

Heavy metals such as Cd pose environmental problems and threats to a variety of organisms. The effects of cadmium (Cd) on the growth and activities of photosystem I (PSI) and photosystem II (PSII) of Chlorella pyrenoidosa were studied. The growth rate of cells treated with 25 and 100 µM of Cd for longer than 48 h were significantly lower than the control, accompanying with the inhibition of photosynthesis. The result of quantum yields and electron transport rates (ETRs) in PSI and PSII showed that Cd had a more serious inhibition on PSII than on PSI. Cd decreased the efficiency of PSII to use the energy under high light with increasing Cd concentration. In contrast, the quantum yield of PSI did not show a significant difference among different Cd treatments. The activation of cyclic electron flow (CEF) and the inhibition of linear electron flow (LEF) due to Cd treatment were observed. The photochemical quantum yield of PSI and the tolerance of ETR of PSI to Cd treatments were due to the activation of CEF around PSI. The activation of CEF also played an important role in induction of non-photochemical quenching (NPQ). The binding features of Cd ions and photosystem particles showed that Cd was easier to combine with PSII than PSI, which may explain the different toxicity of Cd on PSII and PSI.
Augmented video consultations in care homes during the COVID-19 pandemic: a qualitative study
Authors: Ochieng Lorraine , Salehi Mahan , Ochieng Rebecca , Nijhof Dewy , Wong Richard , Gupta Vinay , Prasad Rishabh , Ochieng Bertha 
Published: June 2022

BackgroundThe COVID-19 pandemic necessitated an unprecedented implementation of remote consultations in UK primary care services. Specifically, older adults in care homes had a high need for infection prevention owing to their existing health conditions. GP practices in the East Midlands incorporated augmented video consultations (AVC) with the potential to support remote healthcare assessments for older adults at care homes.AimTo explore GPs' and care home staff's experiences of the use of AVC as a mechanism to perform remote examinations of older adults in care homes.Design & settingQualitative interviews were conducted with GPs and care home staff in the East Midlands, UK, during May–August 2020.MethodA convenience sample of GPs (n= 5), nurses (n= 12), and senior healthcare assistants (n= 3) were recruited using a purposive approach. Data were collected through semi-structured telephone interviews and analysed using framework analysis.ResultsFindings from participants indicated that AVC enabled real-time patient examinations to aid diagnosis and promoted person-centred care in meeting the needs of older adults. The participants also discussed the challenges of video consultations for patients with cognitive impairment and those receiving end-of-life care.ConclusionAVCs show great potential in terms of GPs providing primary care services for care homes. However, healthcare staff must be involved in the development of the technology, and consideration should be given to the needs of older adults with cognitive impairment and those receiving end-of-life care. It is also vital that training is available to encourage confidence and competency in implementing the technology.