bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative analysis of the short-term effects of Babesia microti on rat hepatocytes in both in vitro and in vivo conditions
Authors: Jasik Krzysztof P. , Okla Hubert , Albertyńska Marta , Urbańska-Jasik Danuta , Kleczka Anna , Paluch Jarosław , Pol Przemysław 
Published: December 2022

Abstract
Human babesiosis is a disease reported mainly in North America, while its etiopathogenesis in Europe is less frequently described. However, according to the literature, human babesiosis in Europe is caused not only by Babesia divergens, as previously thought, but also by Babesia microti. Babesiosis is a parasitemia with varied symptoms, and potentially different organs can become dysfunctional during this disease. Since B. microti penetrates the blood during infection, the liver seems to be particularly exposed to these parasites, especially during the first phase of the disease. Considering the above, we aimed to investigate the effect of B. microti merozoites on hepatocytes. The study was carried out under in vitro and in vivo conditions to compare the different effects i.e. to focus on the direct effects of the protozoa on hepatocytes without the influence of associated cells in the living organism, including the immune system. In the study, we analyzed the effects of B. microti (ATCC 30221) on the liver of infected rats and the contact of the same line of B. microti with hepatocytes of the Clone 9 line (ECACC 88072203). The study was conducted at both microscopic and submicroscopic levels.
Using Dinoprostone Slow Release Vaginal Insert for Induction of Labor in Term-Pregnancy with Oligohydramnios: a prospective study from Vietnam
Authors: Nguyen To Nhu Thi Ngo , Le Quang Thanh , Vuong Anh Dinh Bao , Nguyen Phuc Nhon , Nguyen Ngoc Tran Thi , Ho Quang Nhat 
Published: December 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Diurnal variations in quantitative pupillary reactivity in large hemispheric strokes
Authors: Kim Tae Jung , Lee Ji Sung , Park Soo-Hyun , Ko Sang-Bae 
Published: December 2022

Abstract

This study investigated the impact of diurnal variation on quantitative pupillary reactivity in stable patients with large hemispheric strokes. We included 35 patients with large hemispheric stroke without neurological worsening. Quantitative pupillometry was performed every 4 h. Pupillometer values for pupil size, percentage of constriction (%CH), constriction velocity (CV), dilation velocity (DV), and neurological pupil index (NPi) were collected. We evaluated changes in PLR parameters over time using linear mixed model analysis. Among the included patients (n = 35), 951 pupillometer measurements were analyzed. Significant diurnal variations were observed in pupil maximum size, %CH, CV, and DV at 4 AM compared with values at 8 PM. At 4 AM, patients had a significantly larger pupil (right [Rt] 3.59 vs 3.21 mm,P < 0.001; left [Lt] 3.51 vs 3.18 mm,P < 0.001) and higher %CH (Rt 31.48 vs 25.72,P < 0.001; Lt 31.42 vs 24.98,P < 0.001), CV (Rt 1.97 vs 1.68 mm/s,P < 0.001; Lt 1.98 vs 1.65 mm/s,P < 0.001), and DV (Rt 0.97 vs 0.84 mm/s,P < 0.001; Lt 0.94 vs 0.82 mm/s,P = 0.001). Pupillary dynamics demonstrated diurnal variations over 24 h within normal ranges in large hemispheric strokes without neurological worsening.
The Hakcheon-Chogok Megaturbidite in the Miocene Pohang-Youngduk Basin, SE Korea: high-gradient slope failure induced by a giant tsunami wave
Authors: Son Junghee , Cho Soonmi , Hwang In Gul 
Published: December 2022

Abstract
In ancient records, discrimination of specific triggering events from sediment gravity flow deposits is very difficult due to multiple interactions between triggering events and similar sedimentary characteristics. For this study, we introduce the Hakcheon-Chogok Megaturbidite (HCM) in the Miocene Pohang-Youngduk Basin (PYB), providing an unusual opportunity to differentiate the triggering events. This megaturbidite is over 70 m thick in the proximal part and less than 4 m thick in the distal part. The diachronous stratigraphic position and distinct coarser sediments than that of the underlying and overlying successions suggest large-scale slope failure of the fine-grained foreset (more than 2 km 3). The occurrence of exotic granitic boulders, originating from the basement rock more than 1 km west of the present fan-apex are indicative of extreme sea-level run-up. The dispersal pattern and paleo-current direction suggest that the HCM flowed to the northeast, irrespective of the local depositional slope which show a radial distribution from the fan-apex. The distribution pattern almost perpendicular to the strike of the NW-SE trending transfer fault suggests that the HCM was triggered by a reflection flow of giant tsunami wave, resulting in large-scale failure of high-gradient (> 15˚in15˚in slope angle) fan-delta slope.
Confidence intervals from local minimums of objective function
Authors: DERMOUNE Azzouz , OUNAISSI Daoud , SLAOUI Yousri 
Published: December 2022

Abstract
The weighted median plays a central role in the least absolute deviations (LAD). We propose a nonlinear regression using (LAD). Our objective function f (a, l, s) is non-convex with respect to the parameters a, l, s, and is such that for each fixed l, s the minimizer of a → f (a, l, s) is the weighted median med(x(l, s), w(l, s)) of a sequence x(l, s) endowed with the weights w(l, s) (all depend on l, s). We analyse and compare theoretically the minimizers of the function (a, l, s) → f (a, l, s) and the surface (l, s) → f (med(x(l, s), w(l, s)), l, s). As a numerical application we propose to fit the daily infections of COVID 19 in China using Gaussian model. We derive confident interval for the daily infections from each local minimum.
Protein interaction network revealed by quantitative proteomic analysis elucidates TFIIB role in multiple aspects of the transcription cycle
Authors: O'Brien Michael , Ansari Athar 
Published: December 2022

Abstract
TFIIB is implicated in multiple aspects of eukaryotic transcription. To investigate the broader role of TFIIB in transcription, we performed quantitative proteomic analysis of yeast TFIIB. We purified TFIIB from soluble cell lysate and the chromatin fraction. TFIIB purified from the chromatin exhibits a number of interactions that explain its non-canonical roles in transcription. Apart from preinitiation components RNAPII, TFIIF and TFIIH, all three 3’ end processing-termination complexes; CF1, CPF and Rat1, are significantly enriched in chromatin-TFIIB preparation. These results explain the presence of TFIIB at the 3’ end of genes, its role in gene looping, and its newly identified role in termination of transcription. Presence of the Lsm complex as well as TREX complex subunit Sub2 in chromatin-TFIIB opens up the possibility of novel roles of TFIIB in synthesis-decay coupling and nucleocytoplasmic transport of mRNA. This multiplicity of functions may contribute to the preferential targeting of TFIIB during viral pathogenesis.
In-vitro efficacy ofsolanum incanum on Rhipicephalus appendiculatus
Authors: Mugumya obed 
Published: December 2022

Abstract
Herbal remedies have a long history of use for tick control. Solanum incanum ripe fruits are locally used to manage ticks. In this study, the effect of S. incanum fruit extracts on egg hatchability, inhibition of oviposition and larval mortality of Rhipicephalus appendiculatus ticks was investigated. The reproductive efficiency of eggs laid by the ticks was determined. The highest number of eggs laid was recorded in the untreated control, whereas the lowest was in the control treated with amitraz. The number of eggs that were laid after treatment with the plant extract increased with increase in the extract concentration, that is, 20, 10 and 5% was 51.0262, 43.774 and 36.901%, respectively. The control treated with amitraz showed higher efficacy of 98.63556% whereas the untreated control had no effect on the ticks. S. incanum 5% showed higher efficacy of 33.49%, than 10 and 20%, which showed 19.405 and 7.833%, respectively. However, there was no significant differences in efficacies between the fruit extract concentrations. In the results obtained from the larval packet test (LPT), the control treated with amitraz was recorded with the highest mortality of 100% whereas the untreated control showed no significant larval mortality. There was high larval mortality recorded in all the three concentrations of S. incanum, of 100, 50 and 25 mg/ml with 97.969, 98.589 and 95.948%, respectively.
Preparation of some new pyrazolo[1,5-a]pyrimidines and evaluation of their antioxidant, antibacterial (MIC and ZOI) activities, and cytotoxic effect on MCF-7 cell line
Authors: Vahedi Mohammad Mehdi , Asghari Sakineh , Tajbakhsh Mahmood , Mohseni Mojtaba 
Published: December 2022

Abstract
In this work, some novel pyrazolo[1,5-a]pyrimidine derivatives were synthesized and characterized by 1H and 13C NMR, FT-IR and mass spectra data. These compounds exhibited good to high antioxidant activities (DPPH radical scavenging capabilities). Among them, compound 3h showed the highest antioxidant activity (IC50= 15.34 μM) compared to ascorbic acid (IC50= 13.53 μM) as a standard compound. Their antibacterial activities were investigated against two Gram-positive bacteria (B. subtilis, and S. aureus) and two Gram-negative bacteria (P. aeruginosa, and E. coli). The results showed that compound 3i has the best antibacterial activity against Gram-positive B. subtilis (ZOI= 23.0±1.4 mm, MIC= 312 μM). Also, the cytotoxicity of these compounds was assessed against breast cancer cell line (MCF-7), which compound 3f displayed the most cytotoxicity (IC50= 223.9 μM), in contrast with Lapatinib (IC50= 136.6 μM) as a known drug.
WITHDRAWN: Soil-Quality Status in the Reclaimed Land of Arid Region
Authors: Asmoay Ahmed , Mousa Ibrahim , Mohamed Ahmed 
Published: December 2022

Abstract
From the reclaimed land W-the western desert road, W-Mallawi district, El Minya Governorate of Egypt, sixteen soil samples were taken. The samples were physically and chemically analyzed in the USA, to evaluate the purity of the soil and its extracts using a variety of indices, including the soil quality index (SQI), sodium absorption ratio (SAR), base saturation percentage (BS%), summation pollution indexes (Psum), and the comprehensive ecological risk index (RI), which was also compared with guidelines from the food and agriculture organization (FAO). The findings indicate that (1) the pH changed from weakly to moderately alkaline, controlling the adsorption and heavy metal (HM) immobilization. (2) Soils are classified as calcareous if their CaCO3% content is greater than5%, with salinities varying from non-saline to moderately salty. (3) texture of 75% of the soil samples was clay, 25% was clay loam, Kaolinite minerals represented 25% of soil samples, and 75% of samples were montmorillonite minerals, depending on the relation of cation exchange capacity (CEC) with soils. (4) The predominance of SO4, Cl, & Ca is due to the degradation of carbonate and evaporite minerals in soil extracts. (5) exceeding N & P-compounds in extracts indicated the role of phosphate fertilizers, which added to the improvement of soil fertility. (6) Based on SQI readings, soil quality varied from poor to marginal, SAR revealed that there was no risk associated with sodium levels in soils, and according to BS percentages, soil fertility is low. (7) HM Contents had a low influence in soil extracts based on Psum & IR values. (8) Agriculture was more extensive in 2018 and 2020 than in 2022, which could mean that there was no farming throughout this growing season or harvest. (9) Due to the low fertility of the soil in the research location, it was advised not to apply phosphate fertilizers, which causes HM contamination in those soils that affect both animal and human health. Capsule: The Soil in the research sites were newly reclaimed as its quality is pivotal for agriculture.
Delineating groundwater flow-paths in fractured aquifers under hazardous environment using conceptual and geophysical modeling with a case study
Authors: Gemail Khaled S. , Ghoneimi Ashraf , Shebl Salah , Soliman Shokry , El-Sayed Kareem , Saleh Ahmed H. 
Published: December 2022

Abstract
Construction of landfills and open dumping of solid-waste above fracture-controlled aquifers can increase leachate concentrations, contaminating useable surface and shallow groundwater resources. In such cases, it is essential to define the hydrogeological processes and identify the pathways in the fractured aquifer system for contamination migration near the dumpsite. Abu Zaabal Quarry is a typical example where a solid-waste dumpsite was installed directly on fractured basalt around polluted groundwater ponds. To assess the vulnerability conditions in this complex environment, Seismic Refraction Imaging (SRI) and Electrical Resistivity Tomography (ERT) were integrated with the available geological and hydrogeological information for delineating the effective fractured zones and to refine the site conceptual model of the potential pathways associated with solid waste leachates, The constructed resistivity and seismic images help to identify interflow zones in the basaltic protective zone and provide valuable information about the orientation and location of fractures feeding the ponds underneath the dumpsite. The presence of NW vertical fractures, which could provide a vertical hydraulic connection with the lower aquifer, allows the percolation of the leachate in the area around the dumpsite. These results show the advantage of using a combination of various geophysical methods for delineating the strikes of the prevailing fracture patterns and clarifying the pollution situation at typical composite landfills worldwide. The proposed conceptual groundwater flow model is critical for understanding hydrogeological and transport processes in such hazardous environments to achieve sustainable management of groundwater resources.
Correlation between Femoral Head Lateralization and Bone Morphology in Primary Hip Osteoarthritis Investigation performed at Matsudo City General Hospital
Authors: Inagaki Kenta , Hagiwara Shigeo , Kawarai Yuya , Terakawa Hiroakira , Miyamoto Syuichi , Suzuki Chiho , Yamagata Hiroyuki , Nakamura Junnichi , Ohtori Seiji , Iida Satoshi 
Published: December 2022

Abstract
Background
Osteoarthritis (OA) is the most common disease of the hip in adults and its etiology is divided into two groups: primary or idiopathic. Although acetabular dysplasia is the most frequent reason for total hip arthroplasty (THA) in Japan, primary OA has increased recently. Although there are two types of femoral head migration in primary OA: superior and medial, there are some patients with prominent femoral head lateralization. This study aimed to evaluate the relationship between femoral head lateralization and bone morphology of the acetabulum and proximal femur using radiographic factors in primary OA of the hip.
Methods
A retrospective study was conducted between 2008 and 2017 to assess 1308 hips with OA who underwent primary THAs at our institute. The diagnostic criteria for primary OA were Crowe type 1, Sharp’s angle < 45°, and center-edge (CE) angle > 25°. We classified patients with primary OA into two groups based on femoral head lateralization: group L with lateralization or group N without. Radiographic factors included Sharp’s angle, CE angle, acetabular inclination, acetabular depth ratio (ADR), acetabular head index (AHI), and femoral neck-shaft angle (FNA), all examined on an anteroposterior pelvic radiograph. Femoral neck anteversion was calculated using computerized axial tomography.
Results
Primary OA was diagnosed in 210/1308 hips (16.1%) (group L: 112 hips [8.6%]; group N: 98 [7.5%]). Patient demographics were not significantly different. Radiographic factors with observed significant differences between group L and group N were average CE angle (33.0° vs 35.1°, respectively, p = 0.009), ADR (251.6 vs 273.4, p < 0.001), AHI (77.2 vs 80.4, p < 0.001), and FNA (136.9° vs 134.8°, p = 0.012).
Conclusions
This investigation suggests that primary OA with femoral head lateralization demonstrated specific identifiable radiographic characteristics in the acetabulum and proximal femur that might contribute to hip joint instability such as the dysplastic hip.
Trial registration:
The research protocol for this study was approved by the Institutional Review Board of the authors’ affiliated institutions in compliance with the principles of the Helsinki Declaration. Written informed consent was obtained from all participating subjects.
Comparative analysis of frequency ratio, information value and analytical hierarchy process statistical models for landslide susceptibility mapping in Kashmir Himalayas
Authors: Aziz Kainat , Sarkar Shantanu , Sahu Paulami 
Published: December 2022

Abstract
Kashmir Himalaya have witnessed numerous mass wasting events in the last few decades leading to the degeneration of its paleoenvironment. The frequency of these events has increased manifold due its intricate geology and the ongoing developmental work and other anthropogenic activities taking toll on the geo-environment of the valley. This work presents comparative performance of three statistical models in geographic information system (GIS) environment for developing landslide susceptible map (LSM) in and around the Gool area of Ramban district in Jammu and Kashmir union territory, India. The total of 365 landslides were spotted in designated area masking 0.909 km2 of study area. 11 causative factors were evaluated for generating LSM which include slope, aspect, curvature, drainage density, relative relief, distance to fault, distance to road, stream power index, land use land cover, topographic wetness index and lithology. Both the remote sensing data and ancillary datasets has been utilized to compile spatial database. Statistical models like frequency ratio (FR), information value (Inv) and analytical hierarchy process (AHP) are utilized to develop LSM for the area. The sensitivity analysis of the receiver operator curve (ROC) are used to estimate the accuracy of the models. The ROC validated results revealed an accuracy of 0.80, 0.80 and 0.74 for Inv, FR and AHP respectively. The accuracy and efficiency findings suggest that all models can provide a credible map of landslide susceptibility for the study region; however, the FR and Inv models are somewhat more accurate than the AHP model.
The effect of broccoli extract and soy isoflavones on endometrial implants and oxidative parameters in female rat model of endometriosis
Authors: Sabetian Soudabeh , Dialameh Parimah Archin , Tanideh Nader , Gharesifard Behrooz , Ahmadi Moslem , Valibeigi Maryam , Kumar Perikala Vijayananda , Siahbani Sarah , Jahromi Bahia Namavar 
Published: December 2022

Abstract
Background: Endometriosis is a gynecological disorder characterized by the growth of endometrial tissue outside the uterine cavity that is associated with chronic pelvic pain, subfertility and 1-3% increased risk of ovarian cancer. The purpose of the study was to investigate the effect of broccoli extract (BE) alone and in combination with soy isoflavones (SI) on endometrial implants in female rat.
Method: In this study, endometriosis was induced surgically in 40 mature female rats. The rats were divided into 5 groups that were treated by oral gavage for 6 weeks with 0.5 ml of saline 0.9%/day (control group), BE (3000 mg/kg/day), SI (50mg/kg/day), BE/soy isoflavones (BE 3000 mg/kg/day + soy isoflavones 50mg/kg/day) and diphereline as a standard medication (3 mg/kg) intramuscularly. At the end of treatments, the volume and histopathology of the endometrial implants were compared among the 5 groups. The serum levels of oxidative parameters including Superoxide dismutase (SOD), Malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) were also compared between the groups.
Results: The volume of the implants significantly decreased in diphereline group (p = 0.002). The histopathological grade of endometrial implants in BE/SI and diphereline group were significantly decreased compared to the control group (p = 0.001). The serum levels of SOD in BE group were enhanced significantly in comparison to the control group (p = 0.034).
Conclusion: BE in combination with SI decreased the growth and histopathologic grades of transplanted endometrial implants. These herbal compounds may have the potential to be used as an alternative medication for the treatment of endometriosis
Evaluation of the efficiency of WARSEM model and the effect of fillslope information on changing the model results
Authors: Motazeh Alireza Ghomi , Naghdi Ramin , Salimi Edris Taghvaye , AKAY Abdollah Emin 
Published: December 2022

Abstract
As far as numerous researchers concern, the main source of the sediment in forests is roads and its related elements. Due to the importance of the issue, research has been done in this regard exceedingly. M any researchers around the world have studied and evaluated various models. The main purpose of this research was to evaluate the efficiency of the WARSEM model in the study area. In order to achieve this goal, in addition to determining the appropriate correction factor to calibrate the model, evaluating the effect of fillslope factors on the accuracy of the results of WARSEM model in study area was desirable too. Study area was a part of Hyrcanian forests in Northwest of Iran. After recording fixed information along the road, field work done during a whole year because it was decided to use natural rainfall showers in the study process. The field observations indicated that the amount of sediment in the study area was high nevertheless the WARSEM has about 24% overestimate in the study area. So the number of 0.241supposed as calibration index for study area based on bringing the R2, NS and RMSE closer to acceptable values. Also, Results showed that the amount of fillslope sediment does not cause a significant change in the overall results. Therefore, the opinion of the model designers is also valid in the study area.
Flow dynamics and heat transfer characterization of confined multiple jets impinging on a complex surface
Authors: Barbosa F. V. , Teixeira S. F. C. F. , Teixeira J. C. F. 
Published: December 2022

Abstract
Submerged and confined multiple jet impingement is widely implemented in cooling applications since it provides high heat transfer coefficients and heat transfer uniformity. Its performance depends on several variables that make it complex and difficult to control. To understand the physical phenomena and characterize the flow field, an in-depth study using Particle Image Velocimetry (PIV) technique and heat transfer sensors is conducted in this study. The PIV measurements provide relevant data, but their accuracy depends on an effective experimental setup and a careful selection of the most appropriate tracer particles. Therefore, this work presents the experimental apparatus and comprises an analysis of the efficiency of different seeding particles. The results demonstrate the complexity of the jet flow impinging on a step surface, which induces a strong flow reversal that affects the jet flow development and the interaction with the adjacent jets. The large-scale structures induced in the vicinity of the target plate are captured by the PIV, as well as the strong fountain flows generated between the adjacent jets. This increased turbulence leads to an increase in heat transfer which is measured by the heat flux sensors, corresponding to a Nusselt number 25% greater for the case of a 2 D step plate. Through this study, relevant insights for several engineering applications that use multiple jet impingement are provided, highlighting that the combination of PIV and heat flux sensors is appropriate to characterize the jet’s flow dynamics and the heat transfer of this complex process.