bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Neutral and Stable Macrocyclic Mn(II) Complex for MRI Tumor Visualization
Authors: Leone Loredana , Anemone Annasofia , Carella Antonella , Botto Elena , Longo Dario Livio , Tei Lorenzo 
Published: October 2022

AbstractA stable and inert amphiphilic Mn(II) complex based on a bisamide derivative of 1,4‐DO2A (DO2A=tetraazacyclododecane‐1,4‐diacetic acid) was synthesized and its 1H NMR relaxometric behavior was investigated as a function of the magnetic field strength, pH and temperature. The interaction with human serum albumin (HSA) was also studied via relaxometry showing a good relaxivity enhancement at low field (at 1T and 298 K the relaxivity increases from 4.5 mM−1 s−1 of the Mn(II)‐complex to 14.0 mM−1 s−1 of the complex‐HSA supramolecular adduct). In vivo biodistribution and MRI studies highlighted a rapid and mixed renal/liver elimination without spleen accumulation from healthy mice and good contrast enhancing properties in a breast tumor murine model. A comparison with a clinically approved Gd(III) agent (GdBOPTA, Multihance®) underlined that the proposed Mn(II) contrast agent gave comparable tumor contrast enhancement up to 3 hours post‐injection.
Assessment of BRAF V600E (VE1) immunochemistry for the detection of BRAF V600E mutation in non–small cell lung carcinoma cytology specimens
Authors: Garcia Ashley , Rivera Rolon Maria Del Mar , Barkoh Bedia , Chen Wei , Luthra Rajyalakhsmi , Roy‐Chowdhuri Sinchita 
Published: October 2022

AbstractBackgroundNon–small cell lung carcinoma (NSCLC) patients with BRAF V600E–mutated tumors respond to targeted therapy. Testing for BRAF V600E is commonly performed with molecular methods; however, a mutation‐specific VE1 antibody clone can provide an alternative testing option using immunohistochemistry (IHC) for practices using single‐gene testing and in situations when the specimen is inadequate for molecular testing. This study evaluates the usefulness of VE1 IHC in screening for BRAF V600E mutations in NSCLC cytology specimens.MethodsThe authors retrospectively identified cytology cases with a diagnosis of NSCLC that had BRAF V600E IHC performed on cell block sections with the monoclonal VE1 antibody clone. The BRAF V600E IHC results were compared with those of molecular testing performed with an amplicon‐based next‐generation sequencing assay.ResultsThere were 201 NSCLC cases evaluated. The VE1 IHC was positive in seven of seven BRAF V600E–mutated tumors (100%) and was negative in 158 of 158 nonmutated BRAF V600E tumors (100%). Thirty cases did not undergo molecular testing, primarily because of insufficient tissue or because molecular testing was performed on an alternative specimen. Six cases showed equivocal weak/focal staining: Two cases demonstrated BRAF V600E mutations, and four cases were negative by molecular testing.ConclusionsThis study suggests that BRAF V600E IHC can be used reliably to screen NSCLC cytology specimens, and negative results strongly indicate the absence of a BRAF V600E mutation. Having a low threshold for equivocal staining is recommended with molecular confirmation of BRAF V600E for any cases demonstrating weak and/or focal cytoplasmic staining.
Subclass‐specific expression patterns of MET receptor tyrosine kinase during development in medial prefrontal and visual cortices
Authors: Lanjewar Alexandra L. , Jagetia Sonum , Khan Zuhayr M. , Eagleson Kathie L. , Levitt Pat 
Published: October 2022

AbstractMet encodes a receptor tyrosine kinase (MET) that is expressed during development and regulates cortical synapse maturation. Conditional deletion of Met in the nervous system during embryonic development leads to deficits in adult contextual fear learning, a medial prefrontal cortex (mPFC)‐dependent cognitive task. MET also regulates the timing of critical period plasticity for ocular dominance in primary visual cortex (V1). However, the underlying circuitry responsible remains unknown. Therefore, this study determines the broad expression patterns of MET throughout postnatal development in mPFC and V1 projection neurons (PNs), providing insight into similarities and differences in the neuronal subtypes and temporal patterns of MET expression between cortical areas. Using a transgenic mouse line that expresses green fluorescent protein (GFP) in Met+ neurons, immunofluorescence and confocal microscopy were performed to visualize MET‐GFP+ cell bodies and PN subclass‐specific protein markers. Analyses reveal that the MET expression is highly enriched in infragranular layers of mPFC, but in supragranular layers of V1. Interestingly, temporal regulation of the percentage of MET+ neurons across development not only differs between cortical regions but also is distinct between lamina within a cortical region. Further, MET is expressed predominantly in the subcerebral PN subclass in mPFC, but the intratelencephalic PN subclass in V1. The data suggest that MET signaling influences the development of distinct circuits in mPFC and V1 that underlie subcerebral and intracortical functional deficits following Met deletion, respectively.
Exploiting Hydrogen Bonding to Direct Supramolecular Polymerization at the Air/Water Interface
Authors: Argudo Pablo G. , Coelho João Paulo , López‐Sicilia Irene , Guerrero‐Martínez Andrés , Martín‐Romero María T. , Camacho Luis , Fernández Gustavo , Giner‐Casares Juan J. 
Published: October 2022

AbstractFluid interfaces provide an advanced platform for directed self‐assembly of organic composites and formation of supramolecular polymers (SPs). Intermolecular interactions govern the supramolecular polymerization processes, with hydrogen bonding (H‐bonding) as a key interaction in supramolecular chemistry and biology. Two purposefully designed supra‐amphiphiles for assessing the role of H‐bonding were designed and their supramolecular polymerization (SP) at the air/water interface was compared. H‐bonding was confirmed by in situ experimental and computational techniques as the required intermolecular interaction for attaining SPs with well‐defined molecular arrangement. Control of H‐bonding as opposite to traditionally considered interactions (e. g., π‐π stacking) is proposed as a successful strategy for SP at fluid interfaces.
Omicron variant of SARS‐CoV‐2, an epidemiologic assessment of pediatric oncology patients in the Bronx
Authors: Offenbacher Rachel , Gorlick Joshua , Weiser Daniel A. 
Published: October 2022

AbstractBackgroundChildren receiving cytotoxic therapy for cancer have increased risk of infection due to drug‐induced neutropenia and are therefore treated empirically for bacteremia when febrile or ill‐appearing. However, viral infections, which are not frequently life‐threatening, are the most common etiology of febrile episodes and there has been increased effort to differentiate patients who may have a higher risk for adverse outcomes.CaseWe performed a retrospective chart review of pediatric oncology patients diagnosed with COVID‐19 between December 20, 2021 and February 22, 2022 during the Omicron (B.1.1.529) surge at The Children's Hospital at Montefiore, a tertiary care center in the Bronx.ConclusionWe found that no patients in our cohort developed respiratory distress, bacteremia, or serious illness after COVID‐19 infection during the Omicron surge. Future studies will aid in understanding the relationship between community‐acquired infections and bacteremia, and this knowledge can then be applied to develop optimal infection prevention clinical care guidelines.
Binary Jaya‐based channel allocation in cognitive radio networks
Authors: Ali Saoucha Naziha 
Published: October 2022

SummaryCognitive radio is a key solution for wireless networks, as it offers the possibility to cope with spectrum scarcity problem. In this work, we are interested in the channel allocation problem (CA) in cognitive radio networks which is a well‐known NP‐hard optimization problem. CA has been resolved using classic evolutionary approaches such as genetic algorithms (GAs) and particle swarm optimization (PSO). However, the performance of these latters depends strongly on the parameters tuning which is a hard time‐consuming task. Under this umbrella, in this article, we propose a new solution based on Jaya algorithm which is a recent free parameters approach. The performance of our proposed algorithm is compared against GA and PSO under different network topologies, and this in terms of max‐sum‐reward, max‐min‐reward, and max‐proportional‐fair. The obtained simulation results confirm the superiority of our proposed approach under all the tested scenarios.
Cofilin‐Membrane Interactions: Electrostatic Effects in Phosphoinositide Lipid Binding
Authors: Prakash Shikha , Krishna Anjali , Sengupta Durba 
Published: October 2022

AbstractThe actin cytoskeleton interacts with the cell membrane primarily through the indirect interactions of actin‐binding proteins such as cofilin‐1. The molecular mechanisms underlying the specific interactions of cofilin‐1 with membrane lipids are still unclear. Here, we performed coarse‐grain molecular dynamics simulations of cofilin‐1 with complex lipid bilayers to analyze the specificity of protein‐lipid interactions. We observed the maximal interactions with phosphoinositide (PIP) lipids, especially PIP2 and PIP3 lipids. A good match was observed between the residues predicted to interact and previous experimental studies. The clustering of PIP lipids around the membrane bound protein leads to an overall lipid demixing and gives rise to persistent membrane curvature. Further, through a series of control simulations, we observe that both electrostatics and geometry are critical for specificity of lipid binding. Our current study is a step towards understanding the physico‐chemical basis of cofilin‐PIP lipid interactions.
Orthogonal‐ and Path‐Dependent Photo/Acidoswitching in an Eight‐State Dihydroazulene‐Spiropyran Dyad
Authors: Dowds Mathias , Stenspil Stine G. , de Souza João H. , Laursen Bo W. , Cacciarini Martina , Nielsen Mogens Brøndsted 
Published: October 2022

AbstractThe front cover artwork is provided by M. Dowds, S. G. Stenspil, J. H. de Souza, B. W. Laursen, M. Cacciarini and M. Brøndsted Nielsen. The image illustrates switching between the eight logical states of a dihydroazulene–spiropyran dyad, as well as its solvatochromic fluorescence. Cover design by Mathias Dowds. Read the full text of the Research Article at 10.1002/cptc.202200152.
A Short History of Plant Light Microscopy
Authors: Somssich Marc 
Published: October 2022

AbstractWhen the microscope was first introduced to scientists in the 17th century, it started a revolution. Suddenly, a whole new world, invisible to the naked eye, was opened to curious explorers. In response to this realization, Nehemiah Grew, an English plant anatomist and physiologist and one of the early microscopists, noted in 1682 “that Nothing hereof remains further to be known, is a Thought not well Calculated”. Since Grew made his observations, the microscope has undergone numerous variations, developing from early compound microscopes—hollow metal tubes with a lens on each end—to the modern, sophisticated, out‐of‐the‐box super‐resolution microscopes available to researchers today. In this Overview article, I describe these developments and discuss how each new and improved variant of the microscope led to major breakthroughs in the life sciences, with a focus on the plant field. These advances start with Grew's simple and—at the time—surprising realization that plant cells are as complex as animals cells, and that the different parts of the plant body indeed qualify to be called “organs”, then move on to the development of the groundbreaking “cell theory” in the mid‐19th century and the description of eu‐ and heterochromatin in the early 20th century, and finish with the precise localization of individual proteins in intact, living cells that we can perform today. Indeed, Grew was right; with ever‐increasing resolution, there really does not seem to be an end to what can be explored with a microscope. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.
Genetic mapping and confirmation of the Rdm3 locus underlying resistance to southern stem canker in soybean
Authors: Menke Ethan , Widyawan M. Habib , Bachleda Nicole , Buck James , Shearin Zack , Boerma H. Roger , Li Zenglu 
Published: October 2022

AbstractSouthern stem canker (SSC) in soybean [Glycine max (L.) Merrill], caused by the fungal pathogen Diaporthe aspalathi, is a major disease in the southern United States. It can cause up to 80% yield loss in severely infected fields. Plant introduction (PI) 398469, a Maturity Group VI soybean accession from South Korea, is one of six known sources of resistance to SSC. To determine the genetics of the resistance provided by PI 398469, F2:3 and F5‐derived recombinant inbred line (RIL) populations were developed from a cross with the susceptible breeding line G81‐2057. Both populations were phenotyped in the greenhouse using a modified toothpick method. The SSC‐resistant and susceptible DNA bulks consisting of 20 resistant and 18 susceptible families were created from the F2:3 population and genotyped using the Infinium SoySNP50K BeadChips for a bulked segregant analysis (BSA). Based on BSA results, 22 Kompetitive allele specific polymerase chain reaction (KASP) markers were designed to genotype the RIL population. Quantitative trait locus (QTL) analysis identified qRdm_14, on chromosome (Chr) 14 with an R2 of 62%. Linkage mapping in the F2:3 population confirmed the presence of a QTL on Chr 14. F5‐derived RILs derived from G81‐2057 × Crockett were phenotyped for SSC resistance and genotyped with the KASP markers to examine the relationship between Rdm3 from Crockett and qRdm_14 from PI 398469. Both PI 398469 and Crockett carried realleles at the Rdm3 locus for resistance to SSC. The QTL and KASP marker information could be used to screen breeding lines for resistance to SSC.
An investigation of associations between parenting and binge eating across pubertal development in pre‐adolescent and adolescent female participants
Authors: Anaya Carolina , Burt S. Alexandra , Klump Kelly L. 
Published: October 2022

AbstractObjectivePuberty is a period of increased risk for the development of binge eating in female adolescents. Although developmental changes in autonomy‐seeking behaviors and body weight and shape may influence both parenting styles and binge eating during puberty, studies have yet to examine how parenting practices may be differentially associated with youth outcomes depending on developmental stage. The current study examines whether interactions between puberty and parenting are associated with higher levels of binge‐eating symptoms during/after puberty in female youth.MethodsAnalyses used cross‐sectional data from a previous study of disordered eating and puberty in 999 female youth (ages 8–16) and their parents from the Michigan State University Twin Registry. Youth self‐reported binge eating, pubertal development, and perceived parental care and overprotection. Both parents and youth reported on parent–child conflict. Mixed linear models were used to examine whether pubertal development moderates the strength of associations between parenting (parent–child conflict, parental care, and parental overprotection) and offspring binge eating.ResultsAlthough higher levels of parental overprotection and conflict, and lower levels of parental care were all significantly associated with binge eating, none of the associations were significantly moderated by pubertal development or age.DiscussionThe quality of the parent–child relationship is significantly associated with binge eating in female youth regardless of developmental stage, highlighting the need for targeting harmful parenting strategies during adolescent eating disorder intervention.Public SignificanceThis is the first study to examine whether parenting/binge‐eating associations in female participants differ across pubertal development. In a large population‐based sample, we found lower parental care, higher parent–child conflict, and higher parental overprotection were all associated with higher levels of binge eating. Notably, associations did not differ across pubertal stage or age, suggesting that parenting is significantly associated with binge eating, regardless of developmental stage.
Relationships between eating disorder symptoms, muscle dysmorphia symptoms, and suicidal ideation: A random intercepts cross‐lagged panel approach
Authors: Grunewald William , Troop‐Gordon Wendy , Smith April R. 
Published: October 2022

AbstractObjectiveEating disorder (ED) symptoms correlate with suicidality; yet the strength of these relationships in men is unclear. Muscle dysmorphia (MD) symptoms may reflect a more accurate index of body‐related concerns for men, as they better target muscularity concerns typical of men. However, no studies have tested a model in which ED/MD symptoms and suicidality are simultaneously examined. We longitudinally tested whether ED/MD symptoms were related to suicidal ideation among a community sample of men.MethodsMen with MD symptoms (N = 272) were recruited to complete three surveys over 6 weeks. A random intercepts cross‐lagged panel model tested predictive associations between ED/MD symptoms and suicidal ideation, while disaggregating between/within‐person variance.ResultsED/MD symptoms were significantly associated with suicidal ideation at the between‐subjects level (ED: b = .04; MD: b = .09) and showed significant within‐wave covariances with suicidal ideation (ED: b = .02–.04; MD: b = .02–.05). Those who experienced increases in ED symptoms showed increased suicidal ideation at the next wave (b = .32). Those who experienced increases in suicidal ideation showed increases in MD symptoms at the next wave (b = .85).DiscussionResults highlight ED symptoms as a potential risk factor for suicidal ideation among men. Further, suicidal ideation predicted MD symptoms. ED symptoms may create intra‐ and interpersonal distress predicting suicidal ideation. Suicidal ideation may lead to muscle‐building behaviors to cope with suicidal thoughts. Clinicians should assess for suicidal ideation among men at risk for MD/EDs, and for MD symptoms among those reporting suicidal ideation.Public SignificanceEating disorder (ED) symptoms are related to suicidality, but these relationships are understudied among men. Since men report concerns surrounding muscularity, muscle dysmorphia (MD) may be a better ED index for this population. However, little research has investigated relationships between ED symptoms, MD symptoms, and suicidality among men. This study investigated relationships between ED/MD symptoms and suicidality among 272 men. Results may inform clinical assessment, treatment, and classification of MD.
Mapping socio‐ecological systems in Idaho: Spatial patterns and analytical considerations
Authors: Aho Ken , Parsons Susan , Castro Antonio J. , Lohse Kathleen A. 
Published: October 2022

AbstractPolicy interest in socio‐ecological systems has driven attempts to define and map socio‐ecological zones (SEZs), that is, spatial regions, distinguishable by their conjoined social and bio‐geo‐physical characteristics. The state of Idaho, USA, has a strong need for SEZ designations because of potential conflicts between rapidly increasing and impactful human populations, and proximal natural ecosystems. Our Idaho SEZs address analytical shortcomings in previously published SEZs by: (1) considering potential biases of clustering methods, (2) cross‐validating SEZ classifications, (3) measuring the relative importance of bio‐geo‐physical and social system predictors, and (4) considering spatial autocorrelation. We obtained authoritative bio‐geo‐physical and social system datasets for Idaho, aggregated into 5‐km grids = 25 km2, and decomposed these using principal components analyses (PCAs). PCA scores were classified using two clustering techniques commonly used in SEZ mapping: hierarchical clustering with Ward's linkage, and k‐means analysis. Classification evaluators indicated that eight‐ and five‐cluster solutions were optimal for the bio‐geo‐physical and social datasets for Ward's linkage, resulting in 31 SEZ composite types, and six‐ and five‐cluster solutions were optimal for k‐means analysis, resulting in 24 SEZ composite types. Ward's and k‐means solutions were similar for bio‐geo‐physical and social classifications with similar numbers of clusters. Further, both classifiers identified the same dominant SEZ composites. For rarer SEZs, however, classification methods strongly affected SEZ classifications, potentially altering land management perspectives. Our SEZs identify several critical regions of social–ecological overlap. These include suburban interface types and a high desert transition zone. Based on multinomial generalized linear models, bio‐geo‐physical information explained more variation in SEZs than social system data, after controlling for spatial autocorrelation, under both Ward's and k‐means approaches. Agreement (cross‐validation) levels were high for multinomial models with bio‐geo‐physical and social predictors for both Ward's and k‐means SEZs. A consideration of historical drivers, including indigenous social systems, and current trajectories of land and resource management in Idaho, indicates a strong need for rigorous SEZ designations to guide development and conservation in the region. Our analytical framework can be broadly applied in SES research and applied in other regions, when categorical responses—including cluster designations—have a spatial component.
A Multicomponent Approach to Imidazo[2,1‐b]thiazole Derivatives by Sequential PdI2/KI‐Catalyzed Deprotective Oxidative Aminocarbonylation – Dearomative Cyclization – Aromatization
Authors: Veltri Lucia , Amuso Roberta , Prestia Tommaso , Vitale Paola , Gabriele Bartolo 
Published: October 2022

AbstractWe report a direct, multicomponent approach to an important class of fused‐ring heterocycles, namely, imidazo[2,1‐b]thiazole derivatives starting from simple and readily available building blocks (N‐Boc‐N‐propargylthiazol‐2‐amines, carbon monoxide, a secondary amine, and oxygen). The process takes place under the catalysis of the simple PdI2/KI system under relatively mild conditions (100 °C under 20 atm of a 4 : 1 mixture of CO‐air), and leads to 2‐(imidazo[2,1‐b]thiazol‐5‐yl)‐N,N‐dialkylacetamides in yields up to 87 %. The mechanistic pathway leading to carbonylated imidazo[2,1‐b]thiazole occurs through an ordered sequence of steps, involving amine‐induced N‐deprotection, oxidative aminocarbonylation of the triple bond (with Csp−H activation and formation of a 2‐ynamide intermediate), dearomative cyclization (by intramolecular conjugate addition of thioazolic nitrogen to the 2‐ynamide moiety) and aromatization by proton‐shift isomerization. When starting from N‐Boc‐N‐(propargyl)benzo[d]thiazol‐2‐amine, tricyclic benzimidazothiazoles were formed in moderate yields.
Stereoselective Synthesis of Highly Functionalized Structures from α‐Bromo α’‐Sulfinyl Ketones
Authors: Salom‐Roig Xavier J. 
Published: October 2022

AbstractCrystallization‐Induced Diastereomer Transformation (CIDT) of α‐bromo‐α’‐(R)‐sulfinylketones is reported. The utility of these 1,2‐difunctionalized synthons is convincingly demonstrated by their facile conversion into cyanohydrins and bromohydrins. Furthermore, bromohydrins can be converted into complex arrangements of functionality and stereochemistry through SN2‐like inter and intramolecular transformations.