bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selection of red fluorescent protein for genetic labeling of mitochondria and intercellular transfer of viable mitochondria
Authors: Taiko Isamu , Takano Chika , Nomoto Masayuki , Hayashida Shingo , Kanemaru Kazunori , Miki Toshio 
Published: September 2022

Abstract
The phenomenon of intercellular mitochondrial transfer has attracted great attention in various fields of research, including stem cell biology. Elucidating the mechanism of mitochondrial transfer from healthy stem cells to cells with mitochondrial dysfunction may lead to the development of novel stem cell therapies to treat mitochondrial disorders, among other advances. To visually evaluate and analyze the mitochondrial transfer process, dual fluorescent labeling systems are often used to distinguish the mitochondria of donor and recipient cells. Although enhanced green fluorescent protein (EGFP) has been well-characterized for labeling mitochondria, other colors of fluorescent protein have been less extensively evaluated in the context of mitochondrial transfer. Here, we generated different lentiviral vectors with mitochondria-targeted red fluorescent proteins, including DsRed, mCherry mKOκ, and TurboRFP. Among these proteins, mitochondria-targeted DsRed and its variant mCherry often generated bright aggregates in the lysosome while other proteins did not. We further validated that TurboRFP-labeled mitochondria were successfully transferred from amniotic epithelial cells, one of the candidates for donor stem cells, to mitochondria-damaged recipient cells without losing the membrane potential. Our study provides new insight into the genetic labeling of mitochondria with red fluorescent proteins, which may be utilized to analyze the mechanism of intercellular mitochondrial transfer.
The risk of ocular adverse events after anti-PD-1/PD-L1 antibodies is dose-independent: a prospective observation report
Authors: Sato Maho , Kubono Hirohisa , Yamashita Kazuya , Nagamoto Takashi , Ofuji Yoshiko , Fukumoto Ryuki , Akagi Hideko , Kawamura Mari , Suzuki Kotaro 
Published: September 2022

Abstract
Background
Immune checkpoint inhibitors (ICIs) cause ICI-related adverse events (irAEs) such as dry eye and uveitis, sometimes necessitating the suspension of ICI treatment. To the best of my knowledge, this is the first prospective study on ocular irAEs. In this study, we aimed to identify early changes in ocular irAE and control conditions without suspending ICI treatment.
Methods
22 participants who began ICI treatment between July 2020 and July 2021 in Keiyu Hospital, Japan (3 women and 19 men; age, 69.1 ± 7.9 years, range, 53–83 years) were prospectively evaluated. The patients underwent ocular examinations before and 1, 3, and every 2 months after the initial dose of ICI. Examinations included measurement of aqueous flare and central choroidal thickness (CCT).
Results
Among the 22 participants, 6 developed systemic irAEs. Of these 6 patients, one experienced ocular pain after ICI treatment, likely due to dry eye disease. We did not observe any change in CCT or aqueous flare, even in patients with systemic AEs. This result suggests that ocular irAE is dose- independent, and we cannot predict whether the patient have risks of presenting irAE by following up ocular findings.
Conclusions
Ocular irAEs occur dose-independently.
A Novel walk-forward NLP-based approach for generating industry-specific lexicons from news articles
Authors: Sudhakar K 
Published: September 2022

Abstract
The Press releases are a valuable resource for financial traders, and scholars have long exploited them to construct algorithmic stock price predictions. We present novel walk-forward NLP-based approach for generating industry-specific lexicons from news articles, with the goal of dynamically capturing the relationship between words used in these documents and daily stock price fluctuations .We also create a binary classification system that uses our lexicons to forecast the amount of future price fluctuations for individual firms. Then, using press material released by globally famous sources and collected within the Financial Times dataset, we test our methodology in an experimental research conducted on three different industries of the NIFTY-50 Stock Market Data. The prediction accuracy allows us to evaluate the relationship between words and prices as well as estimate our lexicons' prediction potency.
Association between the weight-adjusted-waist index and cardiovascular disease among US adults: Results from the National Health and Nutrition Examination Survey 2009-2016
Authors: Xie Feng , Li Meng , Li Kai , Wu Yanqing 
Published: September 2022

Abstract
Background
As a new obesity-related index, the weight-adjusted-waist index (WWI) appears to be a good predictor of cardiovascular disease (CVD) in East Asian populations. This study aimed to evaluate the association between WWI and the risk of CVD in United States (US) adults.
Methods
The data were obtained from the 2009–2016 National Health and Nutrition Examination Survey (NHANES). WWI was calculated as waist circumference divided by the square root of weight, and CVD was ascertained based on self-reported physician diagnoses. Multivariable regression analysis and subgroup analysis were performed to evaluate the association between WWI and CVD.
Results
A total of 21,040 participants were included, with the mean age being 47.11 ± 16.79 years. There was a positive linear relationship between WWI and the odds of CVD (P = 0.310). After adjusting for all covariates, each unit of increased WWI was associated with a 48% increased risk of CVD (odds ratio [OR]: 1.48, 95% confidence interval [CI]: 1.25–1.74). Moreover, compared with the lowest quintile (< 10.3 cm/√kg), the multivariable-adjusted OR was 3.18 (95% CI: 1.81–5.60) in the highest quintile (≥ 11.8 cm/√kg). Besides, subgroup analyses showed that stronger associations between WWI and CVD were detected in participants younger than 50 years of age (P for interaction < 0.001).
Conclusions
High levels of WWI were significantly associated with an increased risk of CVD in US adults, particularly in people under 50 years of age. These findings indicate that WWI may be an intervention indicator to reduce the risk of CVD in the general adult population.
Nano-magnetite supported N-heterocyclic carbene-gold complex with pendant ferrocenyl group as a multi- modal anti-breast cancer agent
Authors: Patil Pradnya V. , Bansode Prakash A. , Gajare Shivanand P. , Deshmukh Ajit H. , Tiwari Arpita P. , Khot Vishwajeet M. , Pore Dattaprasad M. , Rashinkar Gajanan S. 
Published: September 2022

AbstractA novel nano-magnetite supported N-heterocyclic carbene-gold complex with pendant ferrocenyl group has been prepared by using a multi-step procedure. The complex displayed significantly higher anticancer activity against the MCF-7 breast cancer cell line with IC50 = 32.38 µg/mL as compared with standard drug 5-fluorouracil (IC50 = 25.29 µg/mL) in the MTT assay. The in-ovo antiangiogenic activity of the complex using chicken chorioallantoic membrane assay showed significant decrease in secondary blood vessels indicating that it is efficient angiogenesis inhibitor. The hyperthermia studies of the complex revealed a significant specific absorption rate (SAR) value of 246.974 Wg-1 with therapeutic temperature of 46.8 oC.
Association between muscle mass/strength loss and healthcare costs/quality of life for patients with colorectal cancer: results from the ongoing longitudinal EnCoRe Study
Authors: Beaudart Charlotte , Drost Ruben M W A , Evers Silvia M A A , Paulus Aggie T G , Weijenberg Matty P , Kenkhuis Marlou-Floor , Hiligsmann Mickaël 
Published: September 2022

AbstractPurpose:To assess the associations between muscle parameters of colorectal cancer (CRC) patients and costs/Quality-Adjusted Life-Years (QALYs).Methods:CRC patients from the ‘Energy for Life after ColoRectal Cancer’ (EnCoRe) study in the Netherlands were included. Muscle strength and mid-upper arm muscle circumference (MUAMC) were considered and two-year total societal costs and QALYs were measured. Linear multiple regressions were used to measure the associations between baseline muscle parameters and total two-year costs/QALYs. Linear mixed-model regressions were used to assess? the associations between muscle parameters evolution and total two-year costs/QALYs.Results:194 (133 men, 61 women; mean age 65.9 years) participants were included in the analyses. Lower values of muscle strength and MUAMC at baseline seem to be predictive of higher outpatient costs and higher treatment costs, respectively. In longitudinal analyses, the decrease of muscle strength over two years was associated with higher outpatient cost and the decrease of MUAMC was associated with higher treatment costs. No other significant associations between baseline muscle parameters or evolution of these muscle parameters and costs were found. Regarding QALYs, a two-year decrease of muscle strength seems to be associated with lower QALYs. However, a two-year decline of MUAMC was associated with higher QALYs.Conclusion:Some limited associations were found between muscle parameters and costs/QALYs of CRC patients. The present analyses are however limited by the low variations of muscle parameters over time. Indeed, only a limited decrease of muscle strength was observed in the cohort study. Further longitudinal studies would be needed to investigate the relationships between muscle mass, muscle strength and costs of patients suffering from cancer more profoundly.Implications for cancer survivors:Even if results revealed limited evidence to suggest associations between muscle parameters and costs/QALYs in cancer patients, lower values of muscle strength and MUACM seems to be predictive of higher treatment costs. Targeting muscle parameters in cancer treatment rehabilitation may decrease further treatment costs of CRC patients.
Radiologic-Pathologic Correlation of Non-Mass Enhancement Associated with Malignant Breast Cancer Masses
Authors: Nguyen Derek Le , Lotfalla Mira , Cimino-Mathews Ashley , Habibi Mehran , Ambinder Emily Bayle 
Published: September 2022

Abstract
Objective: To determine pathologic correlation of NME associated directly with malignant index masses on preoperative MRI and determine imaging characteristic predictors of malignant outcomes to inform surgical management.
Methods: This retrospective study reviewed breast MRI examinations performed for extent of disease in newly diagnosed breast cancer patients from 7/1/16 to 9/30/19. Inclusion criteria were limited to patients with a malignant index mass and the presence of NME extending from the mass margins. Fisher’s exact test and Chi-square test were used to analyze cancer, patient, and imaging characteristics associated with the NME diagnosis and determine predictors of malignant outcomes.
Results: This study included 58 patients (mean age 58 ± 12 years). Malignant outcomes for mass-associated NME occurred in 63.8% (37/58) of cases: 43.2% (16/37) in situ and 56.8% (21/37) invasive carcinoma. Longer span of mass-associated NME and lower Ki-67 index were significant predictors of malignant mass-associated NME (p<0.05). The presence of calcifications correlating with mass-associated NME was not a significant predictor of malignant outcomes (p=0.19). Span of disease measured on MRI overestimates the true span of disease on histologic evaluation (p<0.01). There was no significant difference in span of disease measured on mammography when correlating calcifications were present and true span of disease on histologic evaluation (p=0.27).
Conclusion: The majority of mass-associated NME on preoperative MRI is malignant (63.8%). Longer span of mass-associated NME and lower Ki-67 index were significantly associated with malignant outcomes. However, total span of disease measured on MRI tends to overestimate the true histologic span of disease.
Epitranscriptome marks detection and localization of RNA modifying proteins in mammalian ovarian follicles
Authors: Dubuc Karine , Marchais Mathilde , Gilbert Isabelle , Bastien Alexandre , Nenonene Karen E , Khandjian Edward W , Viger Robert S , Robert Claude 
Published: September 2022

Abstract
Background
Most of the resources that support the early development of the embryo are stored in the oocyte. Clearing of maternal resources and activation of the embryonic genome to produce its own mRNA transcripts marks the maternal-to-embryo transition. Dependence on stored mRNA can last from a few hours to several days, depending on animal species. The mechanisms regulating stabilization and recruitment of stored maternal transcripts have not yet been described in full detail but are known to involve reversible polyadenylation and modulation of 3’UTR-mediated elements. RNA epigenetic modifications, new players in this field, have an important role in RNA regulation and stabilization.
Results
The objectives of this study were first to determine if some of post-transcriptional methylation of stored mRNA is greater in oocytes than in somatic cells. We found that m6A, known to be the most prevalent and involved in various aspects of RNA metabolism and physiological functions, is particularly abundant in porcine oocyte mRNA compared to somatic tissues. The second objective was to compare the epitranscriptome machinery, such as methyltransferases (“writers”), binding proteins (“readers”) and demethylases (“erasers”) catalyzing the different process, in follicles and oocytes of different mammalian species by immunofluorescence and confocal microscopy. The expression and localization patterns of these proteins differ between mice, pigs and cows ovaries and oocytes. m5C-associated proteins were generally less abundant. In contrast, m6A-associated proteins were expressed strongly during the early and late stages of folliculogenesis. Transzonal projections were found to contain more granules bearing the m5C mark in mice but both m5C and m6A methylation marks in association with mature oocytes of pigs and cows. Eraser proteins showed the greatest interspecies diversity in terms of distribution in the germinal tissues.
Conclusions
So far, few studies have looked at the oocyte and ovarian epitranscriptomic profile. Our findings indicate that a hitherto unrecognized species-specific layer of transcript regulation occurs at the RNA level and might be consequential during the oocyte transcriptional silencing period.
Analysis of epidemiological trends of and risk factors for tooth loss among 35- to 44-year-old adults in Guangdong, southern China, 1995-2015
Authors: Jiang Linxin , Li Jianbo , Yang Zijing , Huang Xiaoyan , Zhong Xiong , Huang Yi , Liu Bincheng , Wu Linmei , Huang Shaohong , Fan Weihua 
Published: September 2022

Abstract
Background
This study analyzed an epidemiological survey of oral health status among a representative population of adults aged 35–44 years in Guangdong Province from 1995 to 2015. The main purpose of this study was to describe the distribution and trends in tooth loss, derive related risk factors, provide theoretical evidence to support research on tooth loss in adults, help other cities explore comprehensive tooth loss intervention projects, and provide a relevant policy basis for health care planning decisions.
Methods
The data of 1408 participants of three cross-sectional, representative oral epidemiological surveys in Guangdong Province in 1995, 2005, and 2015 were analyzed. Basic demographic information, socioeconomic status, caries and periodontal statuses, personal lifestyle factors, and dental health care behaviors were analyzed by multivariate logistic regression to estimate their associations with tooth loss.
Results
This study found that the mean number of missing teeth (MT) and the prevalence of tooth loss (MT > 0) among adults aged 35–44 years in Guangdong Province did not change significantly in the first decade (1995–2005) but decreased significantly in the second decade (2005–2015) (0.942 and 40.8% in 1995, 0.989 and 42.9% in 2005, and 0.628 and 33.3% in 2015, respectively). The mean number of MT by tooth position was highest for the first molar, followed by the second molar, and both were larger in the mandible than in the maxilla. Education level, caries, and periodontal pocket (periodontal probing depth ≥ 4 mm) in 1995 and 2005 and sex, residence, caries, and periodontal pocket in 2015 were significantly associated with the prevalence of tooth loss.
Conclusions
Although tooth retention has improved in recent decades (1995–2005), measures to prevent tooth loss in adults, especially first molar loss, need to be continued in the future. Moreover, efforts to prevent tooth loss among women, those living in rural areas, and those suffering from caries and periodontal pocket need to be strengthened.
Towards Circular Economy by Valorization of Waste Upholstery Textile Fibers in Fibrous Wood-Based Composites Production
Authors: Suchorab Barbara , Wronka Anita , Kowaluk Grzegorz 
Published: September 2022

Abstract
The aim of the research was to utilize textile waste by adding upholstery fabric fibers with different content (0%, 5%, 10%, and 20% by weight referred to dry wood fibers), to HDF boards and analyze the selected physical and mechanical properties of the obtained boards. Tests of mechanical (modulus of elasticity in bending and of bending strength, surface soundness, internal bond, screw withdrawal resistance) and physical (density profile, swelling in thickness after immersion in water, water absorption) properties were performed. The results show, that the increasing content of textile fibers in HDF panels has the strongest negative influence on mechanical properties, especially internal bond, and screw withdrawal resistance. Modulus of elasticity and modulus of rupture also decrease, but still fulfill the requirements of European standards. No significant influence of raising content of textile fibers on HDF thickness swelling and water absorption has been found. It can be concluded, that, depending on further application of HDF, it is possible to utilize the recovered upholstery textile fibers as an addition to wood fibers when producing HDF panels. It is also a step toward the carbon storage extension, which is fixed in textile fibers.
Statement of Novelty
The present work intends to prove the potential of upcycling the waste upholstery textile fibers by elaborating on MDF-type boards as a high value-added alternative to the current management of this kind of textile waste; this represents a cascading and added-value of this fibrous material in a circular economy, as it generates a new products. The results have shown the high competitiveness of these waste upholstery textile fibrous materials for the production of MDF-type boards.
Cold Plasma Treatment and Peleg Model Application to Chickpea (Cicer arietinum L.)
Authors: Pathan F. L. , Trimukhe A. M. , Deshmukh R. R. , Annapure U. S. 
Published: September 2022

Abstract
Food preservation in the present era seems to be driven by plasma processing due to its efficiency in controlling microorganism at very less temperatures. Legumes are mostly soaked before cooking. Peleg model fitting for six chickpea cultivars after plasma treatment was studied when these were soaked in distilled water at ambient temperature. The cold plasma treatment of 40, 50 and 60 watts each with an exposure time of 10, 15, 20 minutes was applied. The Cold plasma treatment shown an increase in the water absorption rate of chickpea cultivars with respect to changing plasma parameters, but there was no change in the water absorption capacity of the samples. K1 (Peleg rate constant) decreased from 32.3 to 4.3 x 10− 3 (h % −1) for all the six chickpea cultivars consistently as plasma power and treatment time increased, which shown the increase in water absorption rate. K2 (Peleg capacity constant) ranged from 9.4 to 12 x 10− 3 (h % −1) for all the six chickpea cultivars. It did not increase or decrease consistently as plasma power and treatment time increased which shown no effect of plasma treatment on water absorption capacity. Peleg model fitting was found successful to show the correlation of water absorption of chickpea cultivars. The model fit was in the range from R2 ≥ 0.9873 to 0.9981 for all the six chickpea cultivars with increasing soaking time and cold plasma treatment.
Analysis of Ganglion Cell-Inner Plexiform Layer Thickness in Retinal Vein Occlusion with Resolved Macular Edema
Authors: Zheng Zhaoxia , Yan Meng , Li Lu , Zhang Duo , Zhang Lina 
Published: September 2022

Abstract
Purpose. To analyze the retinal ganglion cell-inner plexiform layer (GCIPL) changes in retinal vein occlusion (RVO) eyes with resolved macular edema using optical coherence tomography.
Methods.We compared the average and minimum GCIPL thickness in RVO eyes with fellow eyes and healthy controls including 40 unilateral RVO patients and 48 healthy subjects. The average GCIPL thickness in BRVO eyes was segmented into the affected and opposite area according to the site of lesion, comparing them with corresponding areas in fellow eyes. Furthermore, maximum central macular thickness (CMT), visual acuity (VA), and intravitreal injection times were recorded to investigate their relationship with the GCIPL thickness.
Results.Despite no significant difference in CMT (P = 0.96), the average (P = 0.02 and P < 0.001, respectively) and minimum (both P < 0.001) GCIPL thickness were decreased in RVO eyes with resolved macular edema after treatment in comparison to fellow eyes and healthy eyes. Maximum CMT thickness was negatively correlated with the minimum GCIPL thickness (r = -0.47, P = 0.003). VA and average GCIPL thickness were associated (rs = -0.49, P = 0.002). In a subgroup analysis only included BRVO patients, the opposite area revealed no significant difference between two eyes (P = 0.91) although the affected area in BRVO eyes was decreased (P < 0.001).
Conclusions. A decrease of GCIPL thickness in RVO was observed even after anatomic restoration and associated with VA prognosis. These GCIPL defects could be attributable to systemic risks and RVO itself, not anti-VEGF effects.
Robust superhydrophobic/superoleophilic filter paper for effective separation of oil/water mixtures and surfactant-stabilized water-in-oil emulsion
Authors: Li Yulei , Shi Baoying , Luan Xiayu , Hao Zhanhua , Wang Yufeng 
Published: September 2022

Abstract
Recently, the super-wetting materials for oil/water separation have attracted great attention. Developing a low-cost and high-efficient filter material that is challenging to separate oil/water mixtures and stable water/oil emulsions efficiently. In this paper, Nano titanium dioxide is hydrophobically modified with γ-aminopropyltriethoxysilane (KH550), and then added with polydimethylsiloxane to make stable superhydrophobic coating. A coated filter paper is obtained by roll coating. The water contact angle of the coated filter paper reaches more than 152°, and the oil droplets are quickly absorbed. The superhydrophobic/superoleophilic filter paper has the advantages of environmental protection, simple operation, low-cost, and can be used in continuous industrial production. The superhydrophobic filter paper is used for the separation of various immiscible oil-water mixtures, the separation efficiencies are more than 99.5%. In addition, the superhydrophobic filter paper can effectively separate various surfactant-stabilized water/oil emulsions. After 10 cycles, the separation effect is still satisfactory. Especially when the proportion of water in water/oil emulsion is as high as 20%, the separation efficiency can still be higher than 97.5%. The results show that the superhydrophobic filter paper has good oil-water separation ability, and has broad prospects in industrial applications.