bluuebunny/crossref_metadata_2025_split huggingface dataset:
Allocating vaccines to remote and on-site workers in the tradable sector
Authors: Czaller László , Tóth Gergő , Lengyel Balázs 
Published: March 2022

AbstractVaccination may be the solution to the pandemic-induced health crisis, but the allocation of vaccines is a complex task in which ethical, economic and social considerations are important. The biggest challenge is to use the limited number of vaccines available in a way that protects vulnerable groups, prevents further spread of infection, and reduces economic uncertainty. We argue that once the vaccination of healthcare workers and the most vulnerable groups has been completed, prioritizing the vaccination of on-site workers is important not only to slow the spread of the infection, but also to ensure the smooth running of economic production. We propose a simple economic model where remote and on-site workers are complementary to each other in the short run, thus a negative shock to the supply of either one may decrease the demand for the other, leading to unemployment. By illustrating the model using pre-Covid employment data from Sweden and Hungary, we show that the optimal vaccine allocation between remote and on-site workers in the tradable sector should be based on different proportions depending on the relative infection risk of on-site workers and the degree of vaccine availability. As long as the number of vaccines is limited and on-site workers are at higher risk of infection, they should be preferred in general. However, as more vaccines become available, countries like Sweden, where the share of occupations that can be done remotely is higher shall start immunize remote workers. In Hungary, where on-site work is dominant in the tradable sector, continued vaccination of on-site workers is more beneficial.
Uncertainty in climate change impact studies for irrigated maize cropping systems in southern Spain
Authors: Kamali Bahareh , Lorite Ignacio J. , Webber Heidi A. , Rezaei Ehsan Eyshi , Gabaldon-Leal Clara , Nendel Claas , Siebert Stefan , Ramirez-Cuesta Juan Miguel , Ewert Frank , Ojeda Jonathan J. 
Published: March 2022

AbstractThis study investigates the main drivers of uncertainties in simulated irrigated maize yield under historical conditions as well as scenarios of increased temperatures and altered irrigation water availability. Using APSIM, MONICA, and SIMPLACE crop models, we quantified the relative contributions of three irrigation water allocation strategies, three sowing dates, and three maize cultivars to the uncertainty in simulated yields. The water allocation strategies were derived from historical records of farmer’s allocation patterns in drip-irrigation scheme of the Genil-Cabra region, Spain (2014–2017). By considering combinations of allocation strategies, the adjusted R2 values (showing the degree of agreement between simulated and observed yields) increased by 29% compared to unrealistic assumptions of considering only near optimal or deficit irrigation scheduling. The factor decomposition analysis based on historic climate showed that irrigation strategies was the main driver of uncertainty in simulated yields (66%). However, under temperature increase scenarios, the contribution of crop model and cultivar choice to uncertainty in simulated yields were as important as irrigation strategy. This was partially due to different model structure in processes related to the temperature responses. Our study calls for including information on irrigation strategies conducted by farmers to reduce the uncertainty in simulated yields at field scale.
Mammalian HEMK1 methylates glutamine residue of the GGQ motif of mitochondrial release factors
Authors: Fang Qi , Kimura Yusuke , Shimazu Tadahiro , Suzuki Takehiro , Yamada Ayumi , Dohmae Naoshi , Iwasaki Shintaro , Shinkai Yoichi 
Published: March 2022

AbstractDespite limited reports on glutamine methylation, methylated glutamine is found to be highly conserved in a "GGQ" motif in both prokaryotes and eukaryotes. In bacteria, glutamine methylation of peptide chain release factors 1/2 (RF1/2) by the enzyme PrmC is essential for translational termination and transcript recycling. Two PrmC homologs, HEMK1 and HEMK2, are found in mammals. In contrast to those of HEMK2, the biochemical properties and biological significance of HEMK1 remain largely unknown. In this study, we demonstrated that HEMK1 is an active methyltransferase for the glutamine residue of the GGQ motif of all four putative mitochondrial release factors (mtRFs)—MTRF1, MTRF1L, MRPL58, and MTRFR. In HEMK1-deficient HeLa cells, GGQ motif glutamine methylation was absent in all the mtRFs. We examined cell growth and mitochondrial properties, but disruption of the HEMK1 gene had no considerable impact on the overall cell growth, mtDNA copy number, mitochondrial membrane potential, and mitochondrial protein synthesis under regular culture condition with glucose as a carbon source. Furthermore, cell growth potential of HEMK1 KO cells was still maintained in the respiratory condition with galactose medium. Our results suggest that HEMK1 mediates the GGQ methylation of all four mtRFs in human cells; however, this specific modification seems mostly dispensable in cell growth and mitochondrial protein homeostasis at least for HeLa cells under fermentative culture condition.
Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens
Authors: Saelens Ganna , Planckaert Sören , Martínez-Sernández Victoria , Ubeira Florencio M. , Devreese Bart , Gabriël Sarah 
Published: March 2022

AbstractThe family Anisakidae, mainly represented by Anisakis simplex s.l. and Pseudoterranova decipiens, encompasses zoonotic nematodes infecting many marine fish. Both are responsible for gastrointestinal disease in humans after ingestion of a live larva by consumption of undercooked fish, and, in the case of A. simplex, an allergic reaction may occur after consuming or even handling infected fish. Due to its phylogenetic relatedness with A. simplex, few studies investigated the allergenic potential of P. decipiens, yet none of them focused on its excretory/secretory (E/S) proteins that easily get missed when working solely on extracts from crushed nematodes. Moreover, these E/S allergens remain behind even when the larva has been removed during fish quality processing. Therefore, the aim was to investigate if Anisakis-like allergens could also be detected in both crushed and E/S P. decipiens protein extract using targeted mass spectrometry analysis and immunological methods. The results confirmed that at least five A. simplex allergens have homologous proteins in P. decipiens; a result that emphasizes the importance of also including E/S protein extracts in proteomic studies. Not only A. simplex, but also P. decipiens should therefore be considered a potential source of allergens that could lead to hypersensitivity reactions in humans.
Complete genomic sequencing of canine distemper virus with nanopore technology during an epizootic event
Authors: Lanszki Zsófia , Tóth Gábor E. , Schütz Éva , Zeghbib Safia , Rusvai Miklós , Jakab Ferenc , Kemenesi Gábor 
Published: March 2022

AbstractCanine distemper virus (CDV) endangers a wide range of wild animal populations, can cross species barriers and therefore representing a significant conservational and animal health risk around the globe. During spring to autumn 2021, according to our current estimates a minimum of 50 red foxes (Vulpes vulpes) died of CDV in Hungary, with CDV lesions. Oral, nasal and rectal swab samples were RT-PCR screened for Canine Distemper Virus from red fox carcasses. To investigate in more detail the origins of these CDV strains, 19 complete genomes were sequenced with a pan-genotype CDV-specific amplicon-based sequencing method developed by our laboratory and optimized for the Oxford Nanopore Technologies platform. Phylogenetic analysis of the complete genomic sequences and separately the hemagglutinin gene sequences revealed the role of the Europe lineage of CDV as a causative agent for the current epizootic. Here we highlight the growing importance of fast developing rapid sequencing technologies to aid rapid response activities during epidemics or epizootic events. We also emphasize the urgent need for improved surveillance of CDV, considering the epizootic capability of enzootic strains as reported in the current study. For such future efforts, we provide a novel NGS protocol to facilitate future genomic surveillance studies.
Echogenic swirling pattern, carcinoembryonic antigen, and lactate dehydrogenase in the diagnosis of malignant pleural effusion
Authors: Chian Chih-Feng , Wu Fu-Ping , Tsai Chen-Liang , Peng Chung-Kan , Shen Chih-Hao , Perng Wann-Cherng , Hsu Shih-Chang 
Published: March 2022

AbstractThe echogenic swirling pattern has a role in predicting malignant pleural effusion (MPE). However, its predictive ability is suboptimal, and its clinical utility remains to be defined. The aim of this study was to assess the diagnostic potential of the echogenic swirling pattern combined with pleural carcinoembryonic antigen (CEA) and routine laboratory tests of pleural effusion in MPE. The 80 consecutive patients with underlying malignancy and pleural effusions were recruited. All patients underwent one diagnostic thoracentesis with a cytologic examination of pleural fluid. Our study showed that the sensitivity of echogenic swirling patterns in MPE diagnosis was 67.7%, specificity was 72.2%, positive predictive value (PPV) was 89.4%, and negative predictive value (NPV) was 39.4%. Both CEA and lactate dehydrogenase (LDH) had acceptable sensitivity (71.0% and 60.7%) and specificity (72.2% and 77.8%). Combining the echogenic swirling pattern, pleural CEA, and pleural LDH, the highest sensitivity (95.2%) with a good PPV (86.8) was reached. In this clinical study, we found that combining the echogenic swirling pattern, pleural CEA, and pleural LDH had a higher sensitivity and a high positive predictive value for the diagnosis of MPE. This combination is a potentially suitable method for MPE screening in cancer patients with pleural effusions.
Analysis of keratometric measurements in accordance with axial length in an aged population
Authors: Han Sung Uk , Ryu Soyoung , Jung Hyunjean , Ahn Hyunmin , Kim Sangyeop , Jun Ikhyun , Seo Kyoung Yul , Kim Tae-im 
Published: March 2022

AbstractTo investigate keratometric measurements according to axial length in an aged population. Patients requiring cataract surgery with keratometric measurements from four different ophthalmic devices (autorefractor/keratometer, Scheimpflug imaging, corneal topography/ray-tracing aberrometry, and partial coherence interferometry) between January 2016 and March 2021 were reviewed retrospectively. Cases for which four ophthalmic devices were deployed in the same order a day were included in this investigation. The corneal curvature of the flattest and steepest meridian, mean corneal curvature, corneal astigmatism, steepest axis location, and axial length were evaluated. In total, 250 eyes (137 patients) were included in the analysis. A negative correlation was found between mean corneal curvature and axial length, with correlation coefficients of 0.587, 0.592, 0.588, 0.591, 0.588, and 0.562 for autorefractor/keratometer, Scheimpflug imaging, corneal topography/ray-tracing aberrometry, partial coherence interferometry, total corneal refractive power of Scheimpflug imaging, and simulated keratometry of corneal topography/ray-tracing aberrometry measurements, respectively. No statistically significant differences were found for corneal astigmatism according to axial length. In axial length group of less than 26.0 mm, negative correlation was found between axial length and mean frontal corneal curvature while no correlation was found between axial length and corneal astigmatism. All four ophthalmic devices showed good inter-device reliability for mean corneal curvature but not corneal astigmatism.
Mechanical performance of graphenex/poly(ether ketone ketone) composite sheets by hot pressing
Authors: Wang Q. B. , Jia D. L. , Pei X. H. , Wu X. L. , Xu F. , Ye Z. H. , Wang H. X. 
Published: March 2022

AbstractPolymer composites are gradually replacing traditional metal materials in the fields of aviation, aerospace, automotive and medicine due to their corrosion resistance, light weight and high strength. Moulding technology and organization morphology of polymer composite are key elements affecting the quality of products and their application, so a vacuum hot pressing process for graphenex/poly(ether ketone ketone) (PEKK) (x = 0%, 2%, 3%, 4%, 5%, 6%) composite powders is explored with particularly designed moulding parameters to achieve high conductive properties and good mechanical properties in graphene/PEKK composite sheet with thickness of 1.25 mm and diameter of 80 mm. The vacuum environment ensures that the graphene is not oxidized by air during hot pressing molding, which is essential for achieving conductive property in the graphene/PEKK composite; The hot pressing temperature of each graphene/PEKK composite powder is higher than glass transition temperature but lower than melting temperature, which ensures the graphene/PEKK composite powders is fully compacted and then graphene is fully lapped in the composite sheet. In addition, the graphene/PEKK composite sheet shows conductive property when the graphene content increases to 3wt%, and then the conductivity of the composites increases and then decreases with a peak value at 5wt% with increasing graphene content. By comparing the mechanical properties and microstructure morphology of the graphene/PEKK composite sheets, it was obtained that graphene content has an obvious effect on the mechanical properties of the composites, e.g., the mechanical properties will be increased as the graphene content increasing when graphene content is more than 3%. The graphene distribution law of the composite material with different graphene contents is analysed using a scanning electron microscope (SEM).
Collision of germline POLE and PMS2 variants in a young patient treated with immune checkpoint inhibitors
Authors: Berrino Enrico , Filippi Roberto , Visintin Clara , Peirone Serena , Fenocchio Elisabetta , Farinea Giovanni , Veglio Franco , Aglietta Massimo , Sapino Anna , Cereda Matteo , Visintin Rosella , Pasini Barbara , Marchiò Caterina 
Published: March 2022

AbstractThe onset of multiple and metachronous tumors in young patients induces to suspect the presence of genetic variants in genes associated with tumorigenesis. We describe here the unusual case of a 16-year-old patient who developed a synchronous bifocal colorectal adenocarcinoma with distant metastases. We provide high throughput molecular characterization with whole-exome sequencing (WES) and DNA targeted sequencing of different tumoral lesions and normal tissue samples that led to unveil a germline POLE mutation (p.Ser297Cys) coexisting with the PMS2 c.2174 + 1 G > A splicing mutation. This clinical scenario defines a “POLE-LYNCH” collision syndrome, which explains the ultra-mutator phenotype observed in the tumor lesions, and the presence of MMR deficiency-associated unusual signatures. The patient was successfully treated with immune checkpoint inhibitors but subsequently developed a high-grade urothelial carcinoma cured by surgery. We complement this analysis with a transcriptomic characterization of tumoral lesions with a panel targeting 770 genes related to the tumor microenvironment and immune evasion thus getting insight on cancer progression and response to immunotherapy.
Photo-magnetization in two-dimensional sliding ferroelectrics
Authors: Zhou Jian 
Published: March 2022

AbstractLight–matter interaction is one of the key routes to understanding and manipulating geometric and electronic behaviors of materials, especially two-dimensional materials which are optically accessible owing to their high surface to volume ratio. In the current work, we focus on the recently discovered two-dimensional sliding ferroelectric materials, in which the out-of-plane electric polarization can be switched with a small horizontal translation in one layer. Combining symmetry analysis and first-principles calculations, we predict that light illumination could inject non-equilibrium magnetic moments into the sliding ferroelectrics. Such magnetic moment is composed of both spin and orbital degrees of freedom contributions. We use ZrI2, WTe2, and MoS2 bilayer ferroelectrics to illustrate our theory. Under intermediate light illumination, one can yield non-equilibrium magnetic moments on the order of 0.1–1 μB in these systems, which also depends on the polarization nature of incident light. Furthermore, we show that such photo-injected magnetism changes its sign when the sliding dipole moment switches. This photo-magnetization can be detected by magneto-optical methods (such as Kerr or Faraday effect), which serves as an indicator of sliding ferroelectricity. Hence, one can use an all-optical pump and probe setup to measure and detect the subtle sliding ferroelectric phase.
Economic evaluation and analyses of hospital-based electronic medical records (EMRs): a scoping review of international literature
Authors: Nguyen Kim-Huong , Wright Chad , Simpson Digby , Woods Leanna , Comans Tracy , Sullivan Clair 
Published: March 2022

AbstractDigital transformation is expensive and rarely smooth, often leading to higher costs than anticipated. It is challenging to demonstrate the contribution of digital health investment in achieving the healthcare aims of population health and workforce sustainability. We conducted a scoping review to understand how electronic medical record (EMR) implementations in the hospital setting have been evaluated using cost–benefit analysis (CBA) approaches. The review search resulted in 1184 unique articles, a final list of 28 was collated of which 20 were US-based studies. All studies were published in 2010–2019, with fewer studies published in more recent years. The data used to estimate benefits and costs were dated from 1996 to 2016, with most data from 2000 to 2010. Only three studies were qualified as using cost–benefit analysis approaches. While studies indicated that there is a positive impact from the EMR implementation, the impacts measured varied greatly. We concluded that the current literature demonstrates a lack of appropriate and comprehensive economic frameworks to understand the value of digital hospital implementations. Additionally, most studies failed to align fully to the quadruple aims of healthcare: they focused either on cost savings and/or improved patient outcomes and population health, none investigated healthcare-workforce sustainability.
Engineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range
Authors: Nakagawa Ryoya , Ishiguro Soh , Okazaki Sae , Mori Hideto , Tanaka Mamoru , Aburatani Hiroyuki , Yachie Nozomu , Nishimasu Hiroshi , Nureki Osamu 
Published: March 2022

AbstractThe RNA-guided DNA endonuclease Cas9 is a versatile genome-editing tool. However, the molecular weight of the commonly used Streptococcus pyogenes Cas9 is relatively large. Consequently, its gene cannot be efficiently packaged into an adeno-associated virus vector, thereby limiting its applications for therapeutic genome editing. Here, we biochemically characterized the compact Cas9 from Campylobacter jejuni (CjCas9) and found that CjCas9 has a previously unrecognized preference for the N3VRYAC protospacer adjacent motif. We thus rationally engineered a CjCas9 variant (enCjCas9), which exhibits enhanced cleavage activity and a broader targeting range both in vitro and in human cells, as compared with CjCas9. Furthermore, a nickase version of enCjCas9, but not CjCas9, fused with a cytosine deaminase mediated C-to-T conversions in human cells. Overall, our findings expand the CRISPR-Cas toolbox for therapeutic genome engineering.
Simulations of solar radio zebras
Authors: Karlický M. 
Published: March 2022

Context.Solar radio zebras are used in diagnostics of solar flare plasmas and it is of great importance to construct accurate models to correctly characterize them.Aims.We simulated two zebras to verify their double-plasma resonance (DPR) model.Methods.In our zebra simulations, we used the DPR model in an expanding and compressing part of the loop as well as with the wave propagating along the loop.Results.Using the DPR model in such a loop, we successfully simulated zebras from the 1 August 2010 and 21 June 2011 flares. We found that increasing the density or decreasing the magnetic field in the part of the loop, where zebra-stripe sources are located, the zebra stripes are shifted to higher frequencies and vice versa. In the case of the 21 June 2011 flare, we confirm that small deviations of zebra-stripe frequencies from their mean values can be explained by waves propagating along the loop. We also confirm high values for the gyro-harmonic number of zebra stripes. We explain an inconsistency in the wave velocities derived from the plasma parameters and from the frequency drift in combination with the density model of the solar atmosphere. Finally, we discuss the high values of the gyro-harmonic number found in the studied zebras.
A Rare Pediatric Case of Severe Rhabdomyolysis Owing to Dual Infection
Authors: Akıncı Göktaş Özben , Bektaş Ömer , Öz Tunçer Gökçen , Birsin Özçakar Zeynep , Talim Beril , Tuba Eminoğlu Fatma , Teber Serap 
Published: March 2022

AbstractAim We aimed to report a severe and rare pediatric rhabdomyolysis case associated with a dual viral infection.Case A 13 year-old, healthy girl presented with the complaints of fever, abdominal pain, weakness and dark-colored urine. She was diagnosed with rhabdomyolysis based on clinical signs and laboratory findings. The diagnosis was confirmed by serological tests and real-time polymerase chain reaction for Epstein-Barr virus (EBV) and cytomegalovirus (CMV), respectively. Other potential genetic, metabolic and infectious causes were evaluated meticulously but no evidence was found. This case is also important as it is the first reported case to our knowledge on rhabdomyolysis associated with EBV and CMV co-infection in children.Conclusion The presented case experienced tetraplegia due to the severe muscular damage and muscle power returned to normal range after 3 months. This suggests that EBV and CMV may have exert synergistic effects leading to more severe inflammation and degeneration.
Typ-1-Diabetes und kardiovaskuläre Risikofaktoren bei Kindern und Jugendlichen
Authors: Drozd Irena , Weiskorn Jantje , Lange Karin , Kordonouri Olga 
Published: March 2022

ZusammenfassungDie langfristigen kardiovaskulären Folgen des Typ-1-Diabetes determinieren die Lebenserwartung betroffener Kinder und Jugendlicher. Risikofaktoren für deren Entwicklung sind eine längere Diabetesdauer bzw. Diabetesmanifestation im frühen Lebensalter, Hypertonie, Rauchen, hoher BMI sowie Fettstoffwechselstörungen. Das Management der kardiovaskulären Risikofaktoren bei Kindern und Jugendlichen mit Typ-1-Diabetes beinhaltet zum einen Screeningsmaßnahmen zur frühzeitigen Aufdeckung der pathologischen Veränderungen und zum anderen eine Lebensstilanpassung im Sinne einer ausgewogenen, normokalorischen Ernährung, regelmäßiger Bewegung sowie ggf. einer medikamentösen lipid- bzw. blutdrucksenkenden Therapie.Die Leitlinien und Empfehlungen zur standardisierten Erkennung und Behandlung kardiovaskulärer Risikofaktoren bei jungen Menschen mit Typ-1-Diabetes sind bisher uneinheitlich formuliert und werden deshalb nicht immer im klinischen Alltag angewendet bzw. umgesetzt. Dies führt zu einer relevanten Unterversorgung dieser Patientengruppe. Dem gilt es mit mehr Forschungsansätzen und der Entwicklung eines universellen Prozederes zur Diagnostik und Therapie der kardiovaskulären Risikofaktoren entgegenzuwirken.