bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial genomes of Sternochetus species (Coleoptera: Curculionidae) and the phylogenetic implications
Authors: Zhang Li‐Jie , Li Yu‐Jie , Ge Xue‐Ying , Li Xue‐Yan , Yang Yu‐Xia , Bai Ming , Ge Si‐Qin 
Published: April 2022

AbstractThe three weevil species, Sternochetus gravis, S. mangiferae, and S. olivieri, have all been reported to be serious pests of mango fruits. Morphology, biology, and various management approaches of these economically important weevils have been well studied. However, no mitochondrial genomes have been reported from the genus Sternochetus. Herein, we assembled mitogenomes of all the three Sternochetus species to reveal their mitogenomic characteristics. A DNA library of 350 bp insert size was constructed and sequenced in Illumina's HiSeq 6000 platform with a pair‐end 150 bp sequencing strategy by Novogene. The sequence reads were assembled using GetOrganelle v1.7.1 and the genes were annotated by Geneious Prime 2021.0.3 and MITOS Web Server. Coupled with 61 published mitogenomes from 13 subfamilies of Curculionidae, we reconstructed phylogenetic trees to resolve evolutionary relationships of these closely related species and also examined subfamily‐level classification among Curculionidae. All three mitogenomes are double‐stranded circular molecules with 22 transfer RNA genes, 13 protein‐coding genes (PCGs), 2 ribosomal RNA genes, and 1 noncoding control region as in other insects. Higher interspecific nucleotide divergence (about 10%) of 13 PCGs indicated these three Sternochetus species diverged a long time ago. Phylogenetic analyses using both maximum likelihood and Bayesian inference methods showed that Sternochetus falls into the basal clade of Cryptorhynchini, a tribe in the subfamily Molytinae. The relationship of S. olivieri as a sister species to S. gravis + S. mangiferae was strongly supported. The monophyly of Cryptorhynchini was also well supported whereas Molytinae was suggested to be a polyphyletic group.
QSAR‐driven synthesis of antiproliferative chalcones against SH‐SY5Y cancer cells: Design, biological evaluation, and redesign
Authors: Mellado Marco , Reyna‐Jeldes Mauricio , Weinstein‐Oppenheimer Caroline , Covarrubias Alejandra A. , Aguilar Luis F. , Coddou Claudio , Mella Jaime , Cuellar Mauricio A. 
Published: April 2022

AbstractNeuroblastoma is one of the most frequent types of cancer found in infants, and traditional chemotherapy has limited efficacy against this pathology. Thus, the development of new compounds with higher activity and selectivity than traditional drugs is a current challenge in medicinal chemistry research. In this study, we report the synthesis of 21 chalcones with antiproliferative activity and selectivity against the neuroblastoma cell line SH‐SY5Y. Then, we developed three‐dimensional quantitative structure–activity relationship models (comparative molecular field analysis and comparative molecular similarity index analysis) with high‐quality statistical values (q2 > 0.7;r2 > 0.8;r2pred > 0.7), using IC50and selectivity index (SI) data as dependent variables. With the information derived from these theoretical models, we designed and synthesized 16 new molecules to prove their consistency, finding good antiproliferative activity against SH‐SY5Y cells on these derivatives, with three of them showing higher SI than the referential drugs 5‐fluorouracil and cisplatin, displaying also a proapoptotic effect comparable to these drugs, as proven by measuring their effects on executor caspases 3/7 activity induction, Bcl‐2/Bax messenger RNA levels alteration, and DNA fragmentation promotion.
Synthesis and Strong π–π Interaction of Hexaazatriphenylene Derivatives with Alternating Electron‐Withdrawing and ‐Donating Groups
Authors: Hinokimoto Akira , Ono Toshinori , Fujiwara Makoto , Mori Hiroki , Akiyoshi Ryohei , Nakamura Shinya , Tsutsumi Osamu , Saeki Akinori , Kitagawa Yasutaka , Horike Satoshi , Tanaka Daisuke 
Published: April 2022

AbstractHexaazatriphenylene (HAT) derivatives have attracted wide attention because of their electron‐deficient nature and unique self‐assembly properties. In this work, a facile synthesis method for obtaining HAT derivatives with alternating electron‐withdrawing nitrile and electron‐donating alkoxy groups (HATCNOCn) is proposed. Crystal structure analysis indicated that HATCNOCn forms a one‐dimensional columnar structure via strong π–π interactions. Density functional theory calculations revealed that the edge of HATCNOCn is divided into positively and negatively charged sites owing to the presence of alternating nitrile and alkoxy groups, which would induce strong π–π interactions. Thermal analysis and polarizing optical microscopy revealed that HATCNOCn exhibits columnar liquid‐crystal phases. Time‐resolved microwave conductivity measurements further demonstrated the photoconductive nature of HATCNOCn. The proposed strategy could provide a new strategy for the design of novel organic semiconductive materials.
Combined multivariate statistical and flux balance analyses uncover media bottlenecks to the growth and productivity of Chinese hamster ovary cell cultures
Authors: Yeo Hock Chuan , Park Seo‐Young , Tan Tessa , Ng Say K. , Lakshmanan Meiyappan , Lee Dong‐Yup 
Published: April 2022

AbstractChinese hamster ovary (CHO) cells are widely used for producing recombinant proteins. To enhance their productivity and product quality, media reformulation has been a key strategy, albeit with several technical challenges, due to the myriad of complex molecular mechanisms underlying media effects on culture performance. Thus, it is imperative to characterize metabolic bottlenecks under various media conditions systematically. To do so, we combined partial least square regression (PLS‐R) with the flux balance analysis of a genome‐scale metabolic model to elucidate the physiological states and metabolic behaviors of human alpha‐1 antitrypsin producing CHO‐DG44 cells grown in one commercial and another two in‐house media under development. At the onset, PLS‐R was used to identify metabolite exchanges that were correlated to specific growth and productivity. Then, by comparing metabolic states described by resultant flux distributions under two of the media conditions, we found suboptimal level of four nutrients and two metabolic wastes, which plausibly hindered cellular growth and productivity; mechanistically, lactate and ammonia recycling were modulated by glutamine and asparagine metabolisms in the media conditions, and also by hitherto unsuspected folate and choline supplements. Our work demonstrated how multivariate statistical analysis can be synergistically combined with metabolic modeling to uncover the mechanistic elements underlying differing media performance. It thus paved the way for the systematic identification of nutrient targets for medium reformulation to enhance recombinant protein production in CHO cells.
Market transformations as collaborative change: Institutional co‐evolution through small business entrepreneurship
Authors: Westman Linda , Luederitz Christopher , Kundurpi Aravind , Mercado Alexander Julian , Burch Sarah Lynn 
Published: April 2022

AbstractEntrepreneurship may be one entry point to trigger transformations toward sustainability. Yet, there is limited knowledge on the ability of small‐ and medium‐sized enterprises (SMEs) to play a role in transformation processes, beyond the initial stages of niche innovation. Building on data collected through 125 interviews in Toronto, Vancouver and London, we examine perceived contributions of SME‐led sustainable entrepreneurship to market transformations. Our data show that sustainable entrepreneurs face significant constraints in individually exercising influence over mass markets, as they encounter social forces that generate resistance to change. However, SMEs are able to act collaboratively to shape transformation processes. We propose three mechanisms of institutional co‐evolution that capture these contributions: network learning, collective norm‐construction and collaborative advocacy.
Sustainable competencies of social entrepreneurship for sustainable development: Exploratory analysis from a developing economy
Authors: Ramadani Veland , Agarwal Sucheta , Caputo Andrea , Agrawal Vivek , Dixit Jitendra Kumar 
Published: April 2022

AbstractWith the establishment of social enterprises, the role of social entrepreneurs (SEs) becomes prominent with their exceptional competencies that contribute to the sustainable development (SD) of the environment. Sustainable competencies (SC) assist these entrepreneurs in strengthening their personalities for the present and future development by facilitating entrepreneurial social activities. This study focuses on the investigation of SC of SEs with the help of in‐depth interviews and exploratory factor analysis by collecting the data from SEs belonging to different regions of Uttar Pradesh, India, through a questionnaire. During the analysis of interviews and data, it has revealed that among the eight vital SC, few are entrepreneurial resilience, empathy, emotional intelligence, strategic thinking and so forth, which are crucial for the success of the social entrepreneurial journey. This is the significant study that focuses on the SC required by the present and future generations for the world's SD with humankind and would facilitate the academic institutes and policymakers to inculcate the SC among the generations to create more social enterprises.
Biomechanical assessment of the first dorsal compartment of the wrist: A fresh cadaver study with relevance to de Quervain's disease
Authors: Sugiura Shiro , Matsuura Yusuke , Suzuki Takane , Nishikawa Satoru , Toyooka Takeshi , Ohtori Seiji 
Published: April 2022

AbstractResistance of de Quervain's disease to conservative treatment has been associated with an intertendinous septum in the first compartment. This study aimed to investigate the relationship between intertendinous septum's presence or absence, internal pressure and wrist positon in the first compartment. Fourteen arms were obtained from seven fresh frozen cadavers. A pressure sensor was inserted into the first compartment; if a septum was present, the pressure sensor was inserted into the abductor pollicis longus (APL) side and the extensor pollicis brevis (EPB) side, respectively. Three wrist positions were also tested: neutral, 45° flexion, and 45° extension. Intertendinous septa were present in seven wrists. The average pressure change in the first compartment measured on the EPB side of a present septum was significantly greater than that measured on the APL side of a present septum or where no septum existed: no septum = 54.6 ± 48.3 kPa; septum, EPB = 81.7 ± 76.5 kPa; and septum, APL = 32.8 ± 37.4 kPa. The average pressure change was also significantly greater in the flexion wrist position relative to the neutral and extension positions: neutral = 36.3 ± 58.0 kPa; 45° flexion = 79.5 ± 65.9 kPa; and 45° extension = 50.4 ± 42.6 kPa. Clear relationships existed between (1) the presence of a septum and increased internal pressure on the EPB side in the first compartment and (2) increased internal pressure with the wrist at 45° flexion compared with the neutral and extension position.
Implementing subtype‐specific pre‐clinical models of breast cancer to study pre‐treatment aspirin effects
Authors: Miller Ian S. , Khan Sonja , Shiels Liam P. , Das Sudipto , O' Farrell Alice C. , Connor Kate , Lafferty Adam , Moran Bruce , Isella Claudio , Loadman Paul , Conroy Emer , Cohrs Susan , Schibli Roger , Kerbel Robert S. , Gallagher William M. , Marangoni Elisabetta , Bennett Kathleen , O' Connor Darran P. , Dwyer Róisín M. , Byrne Annette T. 
Published: April 2022

AbstractBackgorundPrior data suggest pre‐diagnostic aspirin use impacts breast tumour biology and patient outcome. Here, we employed faithful surgical resection models of HER2+ and triple‐negative breast cancer (TNBC), to study outcome and response mechanisms across breast cancer subtypes.MethodNOD/SCID mice were implanted with HER2+ MDA‐MB‐231/LN/2‐4/H2N, trastuzumab‐resistant HER2+ HCC1954 or a TNBC patient‐derived xenograft (PDX). A daily low‐dose aspirin regimen commenced until primary tumours reached ~250 mm3 and subsequently resected. MDA‐MB‐231/LN/2‐4/H2N mice were monitored for metastasis utilising imaging. To interrogate the survival benefit of pre‐treatment aspirin, 3 weeks post‐resection, HCC1954/TNBC animals received standard‐of‐care (SOC) chemotherapy for 6 weeks. Primary tumour response to aspirin was interrogated using immunohistochemistry.ResultsAspirin delayed time to metastasis in MDA‐MB‐231/LN/2‐4/H2N xenografts and decreased growth of HER2+/TNBC primary tumours. Lymphangiogenic factors and lymph vessels number were decreased in HER2+ tumours. However, no survival benefit was seen in aspirin pre‐treated animals (HCC1954/TNBC) that further received adjuvant SOC, compared with animals treated with SOC alone. In an effort to study mechanisms responsible for the observed reduction in lymphangiogenesis in HER2+ BC we utilised an in vitro co‐culture system of HCC1954 tumour cells and mesenchymal stromal cells (MSC). Aspirin abrogated the secretion of VEGF‐C in MSCs and also decreased the lymph/angiogenic potential of the MSCs and HCC1954 by tubule formation assay. Furthermore, aspirin decreased the secretion of uPA in HCC1954 cells potentially diminishing its metastatic capability.ConclusionOur data employing clinically relevant models demonstrate that aspirin alters breast tumour biology. However, aspirin may not represent a robust chemo‐preventative agent in the HER2+ or TNBC setting.
Soluble form of CTLA‐4 is a good predictor for tumor recurrence after radiofrequency ablation in hepatocellular carcinoma patients
Authors: Teng Wei , Jeng Wen‐Juei , Chen Wei‐Ting , Lin Chen‐Chun , Lin Chun‐Yen , Lin Shi‐Ming , Sheen I‐Shyan 
Published: April 2022

AbstractBackgroundA soluble form of cytotoxic‐T‐lymphocyte‐antigen‐4 (sCTLA‐4) is a prognostic biomarker for several cancers but remains unclear in HCC patients. The aim of study is to evaluate the predictive role of serum sCTLA‐4 levels for tumor recurrence of chronic hepatis C (CHC)‐HCC patients receiving radiofrequency ablation (RFA).Material and methodA prospective study recruiting 88 CHC‐HCC patients was done between 2013 and 2019. Cox regression analysis was used to determine the predictors of early recurrence. All tests were two‐tailed, and the level of statistical significance was set as p < 0.05.ResultsDuring a median follow‐up of 44.4 months, 53 of the 88 (60.2%) CHC‐HCC patients encountered early recurrence within 2 years. The predictability of sCTLA‐4 for local recurrence (LR) and intrahepatic metastasis (IHM) by 2‐years using AUROC curve analysis were 0.740 and 0.715, respectively. Patients with high sCTLA‐4 levels (>9 ng/ml) encountered shorter recurrence‐free survival (RFS) for LR (log‐rank p = 0.017) but paradoxically longer RFS for IHM (log‐rank p = 0.007) compared to those with low levels (≤9 ng/ml). By multivariate Cox regression analysis, sCTLA‐4 levels and antiviral therapy were independent prognostic factor of early recurrence both in LR and IHM. A combination of baseline sCTLA‐4 and AFP level could improve the predictability of early LR and IHM with specificity of 80.0% and 79.7% and positive predictive value of 63.3% and 67.3%, respectively.ConclusionssCTLA‐4 level is a good predictor for early HCC recurrence with higher levels indicating susceptibility to early LR, but protecting from early IHM.
Morphea in two patients after being infected to and being vaccinated against SARS‐CoV‐2 infection
Authors: Aryanian Zeinab , Balighi Kamran , Hatami Parvaneh , Tootoonchi Nasim Mazloumi , Goodarzi Azadeh , Mohseni Afshar Zeinab 
Published: April 2022

AbstractAlthough the presence of morphea following COVID‐19 has been rarely reported, the development of its generalized form following COVID‐19 vaccination has not been reported yet. Here, we reported the first case of generalized morphea following COVID‐19 vaccination and another similar case following SARS‐Cov‐2 infection. Other etiologic factors were also dealt with.
Chronic recurrent annular neutrophilic dermatosis: A rare entity
Authors: Manaa Linda , Korbi Mouna , Njima Manel , Akkari Hayet , Soua Yosra , Belhadjali Hichem , Zili Jameleddine 
Published: April 2022

AbstractWe report a new case of chronic recurrent annular neutrophilic dermatosis in a woman. Through our observation, we aim to make the clinician aware of this rare entity, in order to consider it among the diagnostic hypotheses of annular dermatosis, with centrifugal, recurrent, and chronic evolution.
Recent progress of flexible aqueous multivalent ion batteries
Authors: Wang Zhenya , Li Yanmei , Wang Jiawei , Ji Runa , Yuan Hao , Wang Yingyu , Wang Hua 
Published: April 2022

AbstractFlexible aqueous batteries have been thriving with the growing demand for wearable and portable electrical devices. In particular, flexible aqueous multivalent ion batteries (FAMIBs), the charge carriers of which include Zn2+, Al3+, Mg2+, and Ca2+, have great potential for development owing to their high safety, high elemental abundance in the Earth's crust, and a multi‐electron redox mechanism with a high theoretical specific capacity. Therefore, for a comprehensive understanding of this developing field, it is necessary to summarize the recent research progress of FAMIBs in a timely manner. Herein, the advancements of the state‐of‐the‐art FAMIBs are reviewed, and the prospects toward this field are also proposed. This study focuses on the rational material and configuration design for FAMIBs in recent studies to achieve high battery performances under deformation conditions, which is elaborated on by classification of the anode, cathode, hydrogel electrolyte, and configurations of FAMIBs. Besides, the electrochemical performance of FAMIBs under flexible conditions is also reviewed from the perspective of their working voltage, specific capacity, and cycling stability. Finally, the approaches to improve the performance of FAMIBs are comprehensively evaluated, followed by the outlook on the challenges and opportunities in future development of FAMIBs.
PROTOCOL: Mapping the scientific knowledge and approaches to defining and measuring hate crime, hate speech, and hate incidents
Authors: Vergani Matteo , Perry Barbara , Freilich Joshua , Chermak Steven , Scrivens Ryan , Link Rouven 
Published: April 2022

AbstractThe overall aim of the review is to map the definitions and measurement tools used to capture the whole spectrum of hate motivated behaviors, including hate crime, hate speech and hate incidents. This will benefit the field of hate studies by providing a baseline that can inform the building of cumulative knowledge and comparative research. The first review objective is to map definitions of hate crime, hate incidents, hate speech, and surrogate terms. Specific research questions underpinning this objective are: (a) How are hate crimes, hate speech and hate incidents defined in the academic, legal, policy, and programming literature?; (b) What are the concepts, parameters and criteria that qualify a behavior as being hate crime, hate incident or hate speech?; and (c) What are the most common concepts, parameters and criteria found across definitions? What are the differences between definitions and the elements they contain? The second review objective is to map the tools used to measure the prevalence of hate crime, hate incidents, hate speech, and surrogate terms. Specific research questions underpinning this objective are: (a) How are definitions operationalised to measure hate crimes, hate speech, and hate incidents?; and (b) How valid and reliable are these measures?
Recommendations for the surveillance of education and employment outcomes in survivors of childhood, adolescent, and young adult cancer: A report from the International Late Effects of Childhood Cancer Guideline Harmonization Group
Authors: Devine Katie A. , Christen Salome , Mulder Renée L. , Brown Morven C. , Ingerski Lisa M. , Mader Luzius , Potter Emma J. , Sleurs Charlotte , Viola Adrienne S. , Waern Susanna , Constine Louis S. , Hudson Melissa M. , Kremer Leontien C. M. , Skinner Roderick , Michel Gisela , Gilleland Marchak Jordan , Schulte Fiona S. M. ,   for the International Guidelines Harmonization Group Psychological Late Effects Group
Published: April 2022

Educational achievement and employment outcomes are critical indicators of quality of life in survivors of childhood, adolescent, and young adult (CAYA) cancer. This review is aimed at providing an evidence‐based clinical practice guideline (CPG) with internationally harmonized recommendations for the surveillance of education and employment outcomes in survivors of CAYA cancer diagnosed before the age of 30 years. The CPG was developed by a multidisciplinary panel under the umbrella of the International Late Effects of Childhood Cancer Guideline Harmonization Group. After evaluating concordances and discordances of 4 existing CPGs, the authors performed a systematic literature search through February 2021. They screened articles for eligibility, assessed quality, and extracted and summarized the data from included articles. The authors formulated recommendations based on the evidence and clinical judgment. There were 3930 articles identified, and 83 of them, originating from 17 countries, were included. On a group level, survivors were more likely to have lower educational achievement and more likely to be unemployed than comparisons. Key risk factors for poor outcomes included receiving a primary diagnosis of a central nervous system tumor and experiencing late effects. The authors recommend that health care providers be aware of the risk of educational and employment problems, implement regular surveillance, and refer survivors to specialists if problems are identified. In conclusion, this review presents a harmonized CPG that aims to facilitate evidence‐based care, positively influence education and employment outcomes, and ultimately minimize the burden of disease and treatment‐related late adverse effects for survivors of CAYA cancers.Lay Summary

A multidisciplinary panel has developed guidelines for the surveillance of education and employment outcomes among survivors of childhood, adolescent, and young adult cancer.

On the basis of evidence showing that survivors are at risk for lower educational achievement and unemployment, it is recommended that all survivors receive regular screening for educational and employment outcomes.
Assessment of 4D flow MRI's quality by verifying its Navier–Stokes compatibility
Authors: Garay Jeremías , Mella Hernán , Sotelo Julio , Cárcamo Cristian , Uribe Sergio , Bertoglio Cristóbal , Mura Joaquín 
Published: April 2022

Abstract4D Flow Magnetic Resonance Imaging (MRI) is the state‐of‐the‐art technique to comprehensively measure the complex spatio‐temporal and multidirectional patterns of blood flow. However, it is subject to artifacts such as noise and aliasing, which due to the 3D and dynamic structure is difficult to detect in clinical practice. In this work, a new mathematical and computational model to determine the quality of 4D Flow MRI is presented. The model is derived by assuming the true velocity satisfies the incompressible Navier–Stokes equations and that can be decomposed by the measurements plus an extra field . Therefore, a non‐linear problem with as unknown arises, which serves as a measure of data quality. A stabilized finite element formulation tailored to this problem is proposed and analyzed. Then, extensive numerical examples—using synthetic 4D Flow MRI data as well as real measurements on experimental phantom and subjects—illustrate the ability to use for assessing the quality of 4D Flow MRI measurements over space and time.