bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ecological Predictors of Pupal Survival in a Common North American Butterfly
Authors: Brackley Allison , Lill John , Weiss Martha 
Published: July 2022

Abstract
All holometabolous insects undergo a pupal life stage, a transformative period during which the insects are immobile and thus particularly vulnerable to both natural enemies and harmful abiotic conditions. For multivoltine species like the silver-spotted skipper [Epargyreus clarus (Cramer) (Lepidoptera: Hesperiidae)], which produces both diapausing and nondiapausing generations throughout much of its range, both the duration of the pupal stage and the ecological challenges faced by pupae can differ among generations. We conducted a set of field experiments to investigate the seasonal and annual variation in pupal mortality for E. clarus pupae experiencing different biotic and abiotic conditions. We also examined the behavioral and ecological factors influencing the construction and persistence of pupal shelters by prepupal larvae. Surprisingly, measures of both cumulative and daily pupal predation were significantly higher during the relatively short (10–14 d) nondiapausing (summer) generations, compared with the diapausing (winter) generations, despite a nearly 20-fold longer pupal duration recorded for the latter. Indirect evidence from field censuses suggested that this intergenerational difference in mortality was due to seasonal variation in consumption of pupae by generalist vertebrate predators. The presence of a shelter increased survival in summer, though not in winter, perhaps because winter pupae were likely to be buried under autumnal leaf litter, regardless of initial shelter status. When constructing their shelters, prepupal E. clarus larvae did not prefer host leaves over nonhost leaves, suggesting that induced preferences are unlikely to play an important role in this process. Despite finding marked differences in the decomposition rates of shelter leaves derived from host vs. nonhost plants, several lines of evidence suggest that these differences are unlikely to impact E. clarus pupal mortality during either the summer or winter generations.
Successful recovery from refractory hypoxia due to right-to-left shunting associated with iatrogenic atrial septal defect after catheter ablation in a patient with a left-ventricular assist device: a case report
Authors: Komeyama Shotaro , Watanabe Takuya , Yamagata Kenichiro , Fukushima Norihide 
Published: July 2022

Abstract

Background
Catheter ablation (CA) has been reported to be an effective therapeutic option for ventricular arrhythmias, even in patients with a left-ventricular assist device (LVAD). However, the issues of right-to-left shunting due to iatrogenic atrial septal defect (iASD) associated with procedures for CA have not been well documented. We describe a rare case of refractory hypoxia associated with right-to-left shunting via iASD after CA through the transseptal approach in an LVAD patient.

Case summary
A 52-year-old Asian man with a continuous-flow implantable LVAD and progressive right ventricular (RV) dysfunction was admitted because of refractory ventricular tachycardia (VT) and subsequent right heart failure. Since VT could not be controlled by intravenous administration of multiple antiarrhythmic drugs, VT ablation via the transseptal approach was performed. Ventricular tachycardia was terminated to the sinus rhythm after VT ablation; however, hypoxia associated with significant right-to-left shunting across the iASD was detected. Intensive medical management, such as an adjusted mechanical ventilator to increase pulmonary vascular compliance and adjustment of LVAD pump speed, as well as the use of intravenous inotropes to support impaired RV function successfully stabilized the haemodynamic and improved hypoxia for the disappearance of right-to-left shunting. Echocardiography at 7 months after CA showed that the significant iASD and right-to-left shunting had disappeared.

Discussion
The evaluation of RV function prior to VT ablation via the transseptal approach is important in the postoperative management of patients with LVAD, because RV dysfunction may cause refractory hypoxia due to iASD.
Complete resection of a pulmonary artery sarcoma involving the pulmonary valve and right ventricle outflow tract: a case report
Authors: Song Wu , Zhong Zhaoji , Liu Sheng 
Published: July 2022

Abstract

Background
Pulmonary artery sarcoma (PAS) is an extremely rare tumour, preferably treated by surgery. However, the surgical management remains largely debatable, as only less than half of patients with PAS can undergo thorough excision.

Case summary
A 32-year-old man with a tumour involving the right ventricle outflow tract, pulmonary trunk extending into the bifurcation, and right pulmonary arteries underwent complete resection using a homologous pulmonary valve and vascular grafts for reconstruction, combined with right pulmonary endarterectomy (PEA) for potential seeding metastasis. Histopathological examination demonstrated undifferentiated pleomorphic sarcoma with surgical margins free of disease. The patient remains asymptomatic, and follow-up computed tomography 5 months after surgery indicated no recurrence or metastasis.

Discussion
Radical resection of a PAS with reconstruction using pulmonary valve allograft and polytetrafluoroethylene vascular grafts is technically feasible and successful. Additionally, PEA may eliminate the potential intima implantation metastasis.
The relationship between physical activity and severity of COVID-19 symptoms in non-hospitalized individuals
Authors: Mitropoulos Alexandros , Goodwill Simon , Copeland Robert , Klonizakis Markos 
Published: July 2022

Abstract

Background
The study explored the relationship between physical activity (PA) behaviour and severity of symptoms in people infected by coronavirus disease 2019 (COVID-19).

Methods
Five hundred and thirty-three people [16% males, mean age: 45 ± 11 years, body mass index (BMI): 23.3 ± 20] took part in the study. All participants were post-COVID-19 infection. An online questionnaire was used to gather data on; participants demographics, comorbidities and treatment, symptomatology of COVID-19, quality of life (QoL) and pre- and post-COVID-19 infection PA.

Results
Logistic regression revealed that only a high BMI (&gt;25) increased the severity of (odds ratio 1.01; 95% confidence interval, 0.99–1.03) symptoms from none to mild-to-moderate. Weekly PA behaviour (min/week) did not affect the primary outcome (symptom severity) as a predictor variable and neither differ (P &gt; 0.05) between symptomatology for both moderate (no symptoms: 181.3 ± 202.1 vs. mild-to-moderate symptoms: 173 ± 210.3) and vigorous (no symptoms: 89.2 ± 147 vs. mild-to-moderate symptoms: 88.9 ± 148.3) PA. QoL (i.e. mobility, self-care, usual activities, pain/discomfort, anxiety/depression and perceived health) was significantly (P &lt; 0.05) worse post-COVID-19 infection.

Conclusions
Our findings did not present an association between PA levels and mild-to-moderate COVID-19 symptoms. However, all participants exceeded the lower limit of the World Health Organization recommended, adult PA dose. This might explain the lack of PA effect, on mild-to-moderate symptoms post-COVID-19 infection. Future studies should explore the effects of PA levels in more severe cases (e.g. hospitalizations) and assess the effectiveness of PA to reduce hospitalizations, and mortality rates as a result of COVID-19 infection.
Conserved developmental trajectories of the cecal microbiota of broiler chickens in a field study
Authors: Kers Jannigje G , Velkers Francisca C , Fischer Egil A J , Stegeman J Arjan , Smidt Hauke , Hermes Gerben D A 
Published: July 2022

Abstract
There is great interest in identifying gut microbiota development patterns and underlying assembly rules that can inform strategies to improve broiler health and performance. Microbiota stratification using community types helps to simplify complex and dynamic ecosystem principles of the intestinal microbiota. This study aimed to identify community types to increase insight in intestinal microbiota variation between broilers and to identify factors that explain this variation. A total of 10 well-performing poultry flocks on four farms were followed. From each flock, the cecal content of nine broilers was collected at 7, 14, and 35 days posthatch. A total of two robust community types were observed using different clustering methods, one of which was dominated by 7-day-old broilers, and one by 35-day-old broilers. Broilers, 14-day-old, were divided across both community types. This is the first study that showed conserved cecal microbiota development trajectories in commercial broiler flocks. In addition to the temporal development with age, the cecal microbiota variation between broilers was explained by the flock, body weight, and the different feed components. Our data support a conserved development of cecal microbiota, despite strong influence of environmental factors. Further investigation of mechanisms underlying microbiota development and function is required to facilitate intestinal health promoting management, diagnostics, and nutritional interventions.
Differential associations between nucleotide polymorphisms and physiological traits in Norway spruce (Picea abiesKarst.) plants under contrasting water regimes
Authors: Hrivnák Matúš , Krajmerová Diana , Kurjak Daniel , Konôpková Alena , Magni Federica , Scaglione Davide , Ditmarová Ľubica , Jamnická Gabriela , Marešová Jana , Gömöry Dušan 
Published: July 2022

AbstractNorway spruce is an ecologically and commercially important species, which currently suffers from climate change and is expected to decline because of heat and drought stress. A manipulative experiment with drought-stressed and well-watered (control) seedlings was established, comprising five populations of Norway spruce coming from a small territory but distributed along a steep elevational transect from 550 to 1280 m.a.s.l. Plants were phenotyped at physiological traits (content of phytohormones and monoterpenes, chlorophyll a fluorescence, gas exchange and hyperspectral indices) and genotyped using ddRAD sequencing. After filtering, 34 127 polymorphisms (SNPs) were retained and used for analysis. Association tests revealed 42 SNP-variable pairs located across 29 scaffolds exhibiting significant associations, among them 20 were found for drought-stressed seedlings and 22 for control (watered) plants. However, there was no consistency between drought-stressed and control plants, no SNP-trait pair was common for both treatments. The study demonstrated that the identified genotype–phenotype associations can only be interpreted in the context of environmental setup preceding the assessment of phenotypic traits.
A distinct P-body-like granule is induced in response to the disruption of microtubule integrity in Saccharomyces cerevisiae
Authors: Hurst Zachary , Liu Wenfang , Shi Qian , Herman Paul K 
Published: July 2022

Abstract
The Processing-body is a conserved membraneless organelle that has been implicated in the storage and/or decay of mRNAs. Although Processing-bodies have been shown to be induced by a variety of conditions, the mechanisms controlling their assembly and their precise physiological roles in eukaryotic cells are still being worked out. In this study, we find that a distinct subtype of Processing-body is induced in response to conditions that disrupt microtubule integrity in the budding yeast, Saccharomyces cerevisiae. For example, treatment with the microtubule-destabilizing agent, benomyl, led to the induction of these novel ribonucleoprotein granules. A link to microtubules had been noted previously and the observations here extend our understanding by demonstrating that the induced foci differ from traditional P-bodies in a number of significant ways. These include differences in overall granule morphology, protein composition, and the manner in which their induction is regulated. Of particular note, several key Processing-body constituents are absent from these benomyl-induced granules, including the Pat1 protein that is normally required for efficient Processing-body assembly. However, these novel ribonucleoprotein structures still contain many known Processing-body proteins and exhibit similar hallmarks of a liquid-like compartment. In all, the data suggest that the disruption of microtubule integrity leads to the formation of a novel type of Processing-body granule that may have distinct biological activities in the cell. Future work will aim to identify the biological activities of these benomyl-induced granules and to determine, in turn, whether these Processing-body-like granules have any role in the regulation of microtubule dynamics.
Pandemic Diaries: Lived Experiences of Loneliness, Loss, and Hope Among Older Adults During COVID-19
Authors: Neves Barbara Barbosa , Colón Cabrera David , Sanders Alexandra , Warren Narelle 
Published: July 2022

Abstract

Background and Objectives
While research on loneliness in later life has increased during the coronavirus disease 2019 (COVID-19) pandemic, we still lack diverse qualitative approaches capturing lived experiences of lonely older adults. Approaches to research with older adults instead of on them are also scant in gerontological literature on loneliness. Through solicited diaries, this study aimed to explore how older Australians who were already lonely before the pandemic managed lockdowns (stay-at-home orders) in Victoria, Australia, which lived through one of the longest lockdowns in the world.

Research Design and Methods
This article is based on qualitative diaries completed by 32 older adults (aged 65 and older). Diaries provide a ‘live’ document where participants become active research partners, recording and sharing their perceptions and experiences. This method is useful to capture sensitive issues, but to the best of our knowledge has not been employed in loneliness studies. Data were thematically analyzed through the identification of themes within and across diaries.

Results
Loneliness was defined by participants as a detrimental absence of companionship and meaningful social interactions. For most, lockdowns exacerbated loneliness, presented new triggers, and upended coping strategies. The disruption of the ‘emotion work’ involved with managing loneliness led to a reconfiguration of response strategies, including through digital technology, which brought both challenges (e.g., digital ageism) and opportunities (e.g., novel communication forms).

Discussion and Implications
Understanding how older people living alone define and respond to loneliness in diverse contexts―for example, before and during a pandemic that restricts social interaction―provides critical insights to inform interventions to tackle loneliness.
Excitation of Rayleigh and Love waves in double-layered half-space
Authors: Zhang Yutong , Zhang Haiming 
Published: July 2022

SUMMARY
In this paper, we report on the seismogram synthesis of Rayleigh and Love waves in horizontally double-layered elastic half-space. For purpose of analytically extracting surface wave components from full-wavefield, we first derive a compact expression of Green’s function in this medium, from which the excitation formulae of Rayleigh and Love waves are then obtained. Secondly, adopting a set of medium parameters close to those of the Earth, and with Rayleigh poles and Love poles solved in complex domain, we manage to compute synthetic seismograms of the two surface waves separately. Their phase-velocity dispersion and particle motion are also explored. For Rayleigh wave, the senior modes behave rather differently from the fundamental mode. While different Love modes share similar patterns, which are generally in conformity with the classic picture.
Context and priorities for health systems strengthening for pain and disability in low- and middle-income countries: a secondary qualitative study and content analysis of health policies
Authors: Briggs Andrew M , Jordan Joanne E , Sharma Saurab , Young James J , Chua Jason , Foster Helen E , Haq Syed Atiqul , Huckel Schneider Carmen , Jain Anil , Joshipura Manjul , Kalla Asgar Ali , Kopansky-Giles Deborah , March Lyn , Reis Felipe J J , Reyes Katherine Ann V , Soriano Enrique R , Slater Helen 
Published: July 2022

Abstract
Musculoskeletal (MSK) health impairments contribute substantially to the pain and disability burden in low- and middle-income countries (LMICs), yet health systems strengthening (HSS) responses are nascent in these settings. We aimed to explore the contemporary context, framed as challenges and opportunities, for improving population-level prevention and management of MSK health in LMICs using secondary qualitative data from a previous study exploring HSS priorities for MSK health globally and (2) to contextualize these findings through a primary analysis of health policies for integrated management of non-communicable diseases (NCDs) in select LMICs. Part 1: 12 transcripts of interviews with LMIC-based key informants (KIs) were inductively analysed. Part 2: systematic content analysis of health policies for integrated care of NCDs where KIs were resident (Argentina, Bangladesh, Brazil, Ethiopia, India, Kenya, Malaysia, Philippines and South Africa). A thematic framework of LMIC-relevant challenges and opportunities was empirically derived and organized around five meta-themes: (1) MSK health is a low priority; (2) social determinants adversely affect MSK health; (3) healthcare system issues de-prioritize MSK health; (4) economic constraints restrict system capacity to direct and mobilize resources to MSK health; and (5) build research capacity. Twelve policy documents were included, describing explicit foci on cardiovascular disease (100%), diabetes (100%), respiratory conditions (100%) and cancer (89%); none explicitly focused on MSK health. Policy strategies were coded into three categories: (1) general principles for people-centred NCD care, (2) service delivery and (3) system strengthening. Four policies described strategies to address MSK health in some way, mostly related to injury care. Priorities and opportunities for HSS for MSK health identified by KIs aligned with broader strategies targeting NCDs identified in the policies. MSK health is not currently prioritized in NCD health policies among selected LMICs. However, opportunities to address the MSK-attributed disability burden exist through integrating MSK-specific HSS initiatives with initiatives targeting NCDs generally and injury and trauma care.
The lncRNA KTN1-AS1 co-regulates a variety of Myc-target genes and enhances proliferation of Burkitt lymphoma cells
Authors: Winkle Melanie , Tayari Mina M , Kok Klaas , Duns Gerben , Grot Natalia , Kazimierska Marta , Seitz Annika , de Jong Debora , Koerts Jasper , Diepstra Arjan , Dzikiewicz-Krawczyk Agnieszka , Steidl Christian , Kluiver Joost , van den Berg Anke 
Published: July 2022

Abstract
Long non-coding RNAs (lncRNAs) are involved in many normal and oncogenic pathways through a diverse repertoire of transcriptional and posttranscriptional regulatory mechanisms. LncRNAs that are under tight regulation of well-known oncogenic transcription factors such as c-Myc (Myc) are likely to be functionally involved in their disease-promoting mechanisms. Myc is a major driver of many subsets of B cell lymphoma and to date remains an undruggable target. We identified three Myc-induced and four Myc-repressed lncRNAs by use of multiple in vitro models of Myc-driven Burkitt lymphoma and detailed analysis of Myc binding profiles. We show that the top Myc-induced lncRNA KTN1-AS1 is strongly upregulated in different types of B cell lymphoma compared with their normal counterparts. We used CRISPR-mediated genome editing to confirm that the direct induction of KTN1-AS1 by Myc is dependent on the presence of a Myc E-box-binding motif. Knockdown of KTN1-AS1 revealed a strong negative effect on the growth of three BL cell lines. Global gene expression analysis upon KTN1-AS1 depletion shows a strong enrichment of key genes in the cholesterol biosynthesis pathway as well as co-regulation of many Myc-target genes, including a moderate negative effect on the levels of Myc itself. Our study suggests a critical role for KTN1-AS1 in supporting BL cell growth by mediating co-regulation of a variety of Myc-target genes and co-activating key genes involved in cholesterol biosynthesis. Therefore, KTN1-AS1 may represent a putative novel therapeutic target in lymphoma.
Ecomechanics and the Rules of Life: A Critical Conduit between the Physical and Natural Sciences
Authors: Ferry Lara A , Higham Timothy E 
Published: July 2022

Abstract
Nature provides the parameters, or boundaries, within which organisms must cope in order to survive. Therefore, ecological conditions have an unequivocal influence on the ability of organisms to perform the necessary functions for survival. Biomechanics brings together physics and biology to understand how an organism will function under a suite of conditions. Despite a relatively rich recent history linking physiology and morphology with ecology, less attention has been paid to the linkage between biomechanics and ecology. This linkage, however, could provide key insights into patterns and processes of evolution. Ecomechanics, also known as ecological biomechanics or mechanical ecology, is not necessarily new, but has received far less attention than ecophysiology or ecomorphology. Here, we briefly review the history of ecomechanics, and then identify what we believe are grand challenges for the discipline and how they can inform some of the most pressing questions in science today, such as how organisms will cope with global change.
Vertebrate Phenological Plasticity: From Molecular Mechanisms to Ecological and Evolutionary Implications
Authors: Aubry Lise M , Williams Cory T 
Published: July 2022

AbstractSeasonal variation in the availability of essential resources is one of the most important drivers of natural selection on the phasing and duration of annually recurring life-cycle events. Shifts in seasonal timing are among the most commonly reported responses to climate change and the capacity of organisms to adjust their timing, either through phenotypic plasticity or evolution, is a critical component of resilience. Despite growing interest in documenting and forecasting the impacts of climate change on phenology, our ability to predict how individuals, populations, and species might alter their seasonal timing in response to their changing environments is constrained by limited knowledge regarding the cues animals use to adjust timing, the endogenous genetic and molecular mechanisms that transduce cues into neural and endocrine signals, and the inherent capacity of animals to alter their timing and phasing within annual cycles. Further, the fitness consequences of phenological responses are often due to biotic interactions within and across trophic levels, rather than being simple outcomes of responses to changes in the abiotic environment. Here, we review the current state of knowledge regarding the mechanisms that control seasonal timing in vertebrates, as well as the ecological and evolutionary consequences of individual, population, and species-level variation in phenological responsiveness. Understanding the causes and consequences of climate-driven phenological shifts requires combining ecological, evolutionary, and mechanistic approaches at individual, populational, and community scales. Thus, to make progress in forecasting phenological responses and demographic consequences, we need to further develop interdisciplinary networks focused on climate change science.