bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction an autophagy-clinical prognostic index in oral cancer
Authors: Jiang Jirui , Zhen Zhanglong , Yin Junhao , Zeng Xiaoli , Shan ZhengNan , Li Shengjiao , Li Jia 
Published: August 2022

Abstract
Background: Autophagy, is a metabolic pathway that occurs in eukaryotic cells and regulated by autophagy-related genes (ARGs).The occurrence and development of many diseases are caused by abnormal autophagy. The purpose of this article is to explore the relationship between autophagy and prognosis of oral cancer, hoping to provide a new way for early diagnosis and guide doctors to make subsequent treatment decisions.Methods: Download the RNA seq and clinical features of 305 oral cancer and 30 non-tumor patients from The Cancer Genome Atlas (TCGA) dataset. Filtered out differential expression autophagy-related genes (ARGs),and gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzed these ARGs. Cox regression analysis filtered out the prognostic ARGs and constructed a risk score models for overall survival (OS) .Divided patients into high-risk and low-risk groups based on median risk score. Kaplan-Meier analyzed the overall survival (OS). Next, receiver operating characteristic (ROC) curve verified the predictive accuracy of the model. Furthermore, we performed stratification analyses to explore the relationship between the prognostic signature and clinicopathological variables. Lastly, we used another date set to verify the model. All data was processed by R (version 3.6.0) and perl (version5.18.4).Results: The K-M plot showed the overall survival rate of the high-risk group was lower than the low-risk group’s (P=2.216e−10). And Cox regression analysis suggested that the autophagy prognostic index was an independent prognostic factor. Further more, the ARGs prognostic model was confirmed in dataset of GSE65858.Conclusion: This study constructed an autophagy-related signature of oral cancer, which can foresee the prognosis of patients. It will open up new prospects for fight against oral cancer.
The MBS Microbial Rapid Detection System for rapid detection of major pathogenic bacteria in feed: Comparison with plate counting method
Authors: Jiang Linlin , Zhang Beiebei , Liu Shuitao , Zhu Lianqin , Zhu Fenghua 
Published: August 2022

AbstractThe current methods for detecting pathogenic bacteria in feed require high technique and take a long time. A Micro Biological Survey (MBS) rapid detection system is a simple, economical and rapid microbial detection method. The purpose of this experiment was to compare the detection ofEscherichia coli(E. coli),Salmonella,Staphylococcus aureus(S. aureus),Listeria monocytogenes(LM), coliform (COLI) and total viable count (TVC) in feed by the MBS rapid microbial detection system and plate counting method (PCM). The results showed that the MBS microbial rapid detection system could detectE. coli, Salmonella, S. aureus, LM, COLI and TVC in artificially contaminated sterilized fish meal, and the quantitative limit were 5.58 cfu/mL, 8.60 cfu/mL, 7.90 cfu/mL, 8.40 cfu/mL, 3.93 cfu/mL and 7.43 cfu/mL, respectively. The quantitative limit of the PCM were 41.30 cfu/mL, 56.70 cfu/mL, 39.00 cfu/mL, 56.00 cfu/mL, 40.00 cfu/mL and 48.00 cfu/mL, respectively. The recovery rates of the MBS microbial rapid detection system forE. coli, Salmonella, S. aureus, LM, COLI and TVC in the concentration range of 101~108cfu/mL were 87.92 ~ 98.42%, 87.19 ~ 90.76%, 89.95 ~ 96.32%, 89.15 ~ 92.93%, 90.95 ~ 97.66% and 90.21 ~ 97.03%, respectively; the recovery rates of the PCM were 5.58%~92.60%, 79.77%~93.48%, 86.33%~94.01%, 83.05%~92.69%, 87.44%~96.58% and 87.09%~94.98%, respectively. The coefficients of variation of the MBS microbial rapid detection system for detectingE. coli, Salmonella, S. aureus, LM, COLI and TVC in sterilized fish meal were 9.83%, 7.18%, 10.28%, 9.55%, 8.03% and 9.21%, respectively. The coefficients of variation of PCM were 14.92%, 13.84%, 15.63%, 12.18%, 13.21% and 14.91%, respectively. When detecting the pathogenic bacteria content in artificially contaminated feed, the MBS rapid microbial detection system was positively correlated with the PCM. When the MBS microbial rapid detection system and PCM were used to detect the collected real feed samples, there was no significant difference in the detection results of the two methods in most of the feed samples. In summary, the quantitative limit, recovery rate and coefficient of variation of the MBS microbial rapid detection system are better than the quantitative limit, recovery rate and coefficient of variation of the plate counting method, which is the most convenient and rapid detection method and is suitable for promotion and application in production lines.
Adipose tissue-derived stromal/stem cells transplantation + cholecalciferol in recent-onset type 1 diabetes patients: twelve months follow up.
Authors: DANTAS Joana Rodrigues , ARAÚJO Débora Batista , SILVA Karina Ribeiro , SOUTO Débora Lopes , PEREIRA Maria de Fátima Carvalho , LUIZ RonirRaggio , CLAUDIO-DA-SILVA Cesar , COURI Carlos Eduardo Barra , MAIOLINO Angelo , REBELATTO Carmen Lúcia Kuniyoshi , DAGA Débora Regina , SENEGAGLIA Alexandra Cristina , BROFMAN Paulo Roberto Slud , BAPTISTA Leandra S. , de OLIVEIRA José Egídio Paulo , ZAJDENVERG Lenita , RODACKI Melanie 
Published: August 2022

Abstract
OBJECTIVE: To evaluate safety and therapeutic effect along 12 months of allogenic adipose tissue-derived stromal/stem cells (ASCs) transplantation+cholecalciferol(VITD) in patients with recent-onset type 1 diabetes (T1D).METHODS: Prospective, phase II, open trial, pilot study in which patients with recent onset T1D received ASCs(1Kgx106 cells) and VITD 2000UI/day for 12 months(group 1) and were compared to controls with standard insulin therapy (group 2). Adverse events, C-peptide area under the curve(CPAUC), insulin dose, HbA1c and frequency of CD4+FoxP3+ T-cells(flow cytometry)were evaluated at baseline(T0), after 3(T3), 6(T6) and 12 months(T12).RESULTS: 11 patients completed the 12 months follow up (7:group 1;4:group 2). Group 1 had lower insulin requirement at T3(0.24+/-0.18vs0.53+/-0.23UI/kg,p=0.04), T6(0.24+/-0.15vs0.66 +/- 0.33 UI/kg,p=0.04) and T12(0.39+/-0.15vs0.74+/-0.29 UI/Kg,p=0.04).HbA1c was lower at T6(6.7+/-0.79vs8.75+/-0.95%,p=0.01), without significant differences at T12(7.3+/-1.11% in group 1vs8.90+/-1.33 in group 2,p=0.16).CPAUC was not significantly different at T0(p=0.07), higher in group 1 at T3(p=0.04) and T6(p=0.006), but similar at T12(p=0.23).Six patients (85,7%) in group 1 were in partial clinical remission(CR) at T6 vs none in group 2,p=0.01,4 remained in remission until 12 months. Patients with partial CR exhibited higher FOX P3 expression in CD4+lymphocytes at T6 and T12(p=0.004 and p=0.02, respectively).VITD levels were higher in patients that underwent partial CR at T6. One patient has a recurrence of a benign teratoma that was surgically removed, not associated to the intervention was observed in a patient from group 1.CONCLUSIONS: ASCs+VITD without immunosuppression was safe and associated lower insulin requirements, a better glycemic control and a transient better pancreatic function in recent onset T1D, but the potential benefits were not sustained. Trial registration : ClinicalTrial.gov NCT03920397
Origin identification of Phoebe zhennan and Phoebe bournei by SPME-GC-MS
Authors: Chen Zhenan , Wu Haoqi , Chen Ruoyi , Xue Xiaoming , Gao Handng 
Published: August 2022

Abstract
Phoebe zhennan S. Lee (Lauraceae) and Phoebe bournei (Hemsl.) Yang are favored for their fine-grain, insect resistance, special fragrance, not easily cracked and deformed, which had been widely used in furniture and construction. Due to the difference in wood quality characteristics and economic value of genus Phoebe species from different origins, it is important to establish a fast, efficient, and reliable method to identify the origin of Phoebe zhennan and Phoebe bournei. Solid phase micro-extraction (SPME) was used as sample preparation approach and gas chromatography-mass spectrometry (GC-MS) technique was employed to analyze the volatile compounds qualitatively. As a result, a total of 40 volatiles were identified by SPME-GC-MS among Phoebe zhennan samples from four origins, while 35 substances identified among Phoebe bournei samples. Significant difference was detected in the volatiles of Phoebe zhennan from different origins except for Guizhou and Yunnan. However, the wood samples of Phoebe bournei from all three origins could be discriminated by GC-MS spectroscopy. This finding indicated that genus Phoebe species from different origins had different chemical composition, which verified that the SPME-GC-MS technique can be used to identify different species of wood with characteristic compounds.
Consequences of an Infinite Fourier Cosine Transform-Based Ramanujan Integral
Authors: Dar S. A. , Kamarujjama M. , Shah W. M. , Daud M. 
Published: August 2022

Abstract
In this paper, we express a generalization of the Ramanujan integral I(α) with the analytical solutions, using the Laplace transform technique and some algebraic relation or a Pochhammer symbol. Moreover, we evaluate some consequences of a generalized definite integral φ * (υ, β , a). The well known special cases appeared whose solutions are possible by Cauchys residue theorem, and many known applications of the integral I(a, β , υ) are discussed by Leib-nitz’s rule for differentiation under the sign of integration. Further, one closed-form evaluation of the infinite series of the 1 F 0 (·) function is deduced. In addition, we establish some integral expressions in terms of the Euler numbers, which are not available in the Gradshteyn and Ryzhik book table. 2010 AMS Classification: 33C05, 33C20, 33B152010 AMS Classification: 33C05, 33C20, 33B15
Fibroblast activation protein imaging in atrial fibrillation: a proof-of-concept study
Authors: Li Lina , Gao Jie , Chen Bi-Xi , Liu Xingpeng , Shi Liang , Wang Yanjiang , Wang Li , Su Pixiong , Yang Min-Fu , Xie Boqia 
Published: August 2022

Abstract

Purpose: Radiolabeled fibroblast activation protein inhibitor (FAPI) imaging has proven feasible in the assessment of ventricular fibrosis in several cardiovascular diseases. However, its value in assessing atrial fibrosis in atrial fibrillation (AF) remains unknown. Methods: Twenty-eight patients with AF and 25 sex-matched controls underwent baseline FAPI PET/CT imaging. Left atrial appendage (LAA) specimens were obtained from seven patients and two lung transplant donors. Atrial pacing beagle dog AF models were constructed by pacemaker implantation. After 8 weeks of pacing, the animals underwent FAPI PET/CT imaging and specimens from the left atrial and right atrial (LA, RA) were collected. FAPI activities in the LA, RA, and LAA were measured by target-to-background ratio (TBR), and the mRNA and protein expression of fibroblast activation protein (FAP) and collagen I were measured by quantitative polymerase chain reaction (PCR) and western blot. Results: FAPI imaging identified increased atrial uptake in 17 patients with persistent AF (PsAF) (85.00%) and 5 with paroxysmal AF (PAF) (62.50%), located in the LA (64.28%), RA (57.14%), and LAA (42.86%). In all atrial structures, patients with PsAF had a higher prevalence of enhanced FAPI uptake than those with PAF (63.33% vs. 33.33%, P = 0.02). LA dilation and B-type natriuretic peptide level were determined as independent factors for increased LAA and RA FAPI uptake, respectively (OR: 1.11 and 1.02, both P < 0.05). All five beagles with AF had increased FAPI uptake in the LA and RA. For both human and animal specimens, FAPI activity was strongly associated with the mRNA and protein levels of FAP, and was closely related to the mRNA expression of collagen I. Conclusion: This preliminary study suggests that FAPI imaging is feasible for assessing activated fibroblasts in the atriums of AF.
Prediction of the incidence of colorectal cancer by routine laboratory tests and anthropometric measurements about metabolism
Authors: Cheng Binglu , Han Xu , Wan Hongjuan , Sun Zhijun , Wang Weisheng 
Published: August 2022

Abstract
Objective To investigate the relationship between metabolic disorders and the morbidity of colorectal cancer (CRC) compared with colorectal adenoma (CRA) and non-neoplastic inpatients, especially to investigate which anthropometric measures were better predictors of CRC. Methods 80 patients with CRC, 80 patients with CRA and 80 non-neoplastic patients were selected from April 2017 to April 2019. The data of routine laboratory tests and anthropometric measurements about metabolism were analyzed retrospectively. Multinomial Logistic regression analysis was used to estimated odds ratio (OR) and 95% confidence interval (CI) for the risk of morbidity of CRC associated with potential risk factors. Results Compared with CRA, OR of CRC was enhanced by weight loss self-reported, anemia and hypoproteinemia. Compared with non-neoplastic controls, diabetes, higher waist-neck ratio (WNR) and The Metabolic syndrome (MetS) identifified by the Chinese Diabetes Society was associated with signifificant increasing risk of all CRC patients. while in male subgroup, OR of CRC was enhanced by diabetes, higher hip circumference, waist-neck ratio (WNR)>2.42 (OR = 2.795, 95%CI: 1.169-6.680), waist-height ratio (WHtR)>0.550 (OR = 3.009, 95%CI: 1.260-7.185) and MetS. In female subgroup, only waist-hip ratio (WHR)>0.890 (multivariable-adjusted OR = 3.354, 95%CI: 1.011-11.129) was significantly associated with increased risk of CRC compared with colorectal adenoma and non-neoplastic controls. The risk factors for CRA were similar to CRC compared with non-neoplastic controls. Conclusion Visceral adipose patients have a high incidence of CRC and CRA. For male, MetS, high level WNR and WHtR caused an increasing risk for CRC. For female, Only WHR is a high-risk factor. The patients with these high risk factors need to strengthen colorectal cancer screening, which is of great significance for the prevention and early detection of colorectal cancer.
Applying the Deep Neural Network to Estimate Future Trend and Uncertainty of Rainfall under Climate Change
Authors: Lin Shiu-Shin , Yeh Wei-Li , Zhu Kai-Yang , Ho Yu-Dong , Wu Wei-Cheng 
Published: August 2022

Abstract
This study proposes a deep neural network (DNN) as a downscaling framework to compare original variables and nonlinear data features extracted by kernel principal component analysis (KPCA). It uses them as learning data for DNN downscaling models to assess future regional rainfall trends and uncertainties in islands with complex terrain. This study takes Taichung and Hualien in Taiwan as examples. It collects data in different emission scenarios (RCP 4.5, RCP 8.5) simulated by two Global Climate Models: ACCESS and CSMK3, in the Fifth Assessment Report (AR5) of the Intergovernmental Panel on Climate Change (IPCC), and monthly rainfall data of case regions from January 1950 to December 2005 in the Central Weather Bureau in Taiwan. DNN model parameters are optimized based on historical scenarios to estimate the trends and uncertainties of future monthly rainfall in the case regions. A multivariate linear regression is used as a baseline model to compare their effectiveness. The simulated results show that by both ACCESS and CSMK3, the dimensionless root mean squared error (RMSE) of KPCA was higher than that of the original variables in Taichung and Hualien. According to the analysis of three-class classification (according to the arrangement in descending power of historical rainfall, the predicted rainfall is divided into three ranges, low, normal, and high, marked by 30% and 70% of monthly rainfall), the wet season rainfall at the two stations is concentrated in the normal range. The probability of rainfall increase will improve in the dry season and will reduce in the wet season in the mid-term to long-term. The future wet season rainfall in Hualien has the highest variability. It ranges from 201 mm to 300 mm, with representative concentration pathways RCP 4.5 much higher than RCP 8.5. The median percentage increase and decrease in RCP 8.5 are higher than in RCP 4.5. This indicates that RCP 8.5 has a greater impact on future monthly rainfall.
Prognostic value of comprehensive typing based on m6A and gene cluster
Authors: Wu Haoming , Feng Jikun , Wu Jundong , Zhong Wenjing , Zouxu Xiazi , Huang Weiling , Huang Xinjian , Yi Jiarong , Wang Xi 
Published: August 2022

Abstract
Background
Triple-negative breast cancer (TNBC) is resistant to targeted therapy with HER2 monoclonal antibodies and endocrine therapy because it lacks the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). TNBC is a subtype of breast cancer with the worst prognosis and the highest mortality rate compared to other subtypes. N6-methyladenosine (m6A) modification is significant in cancer and metastasis because it can alter gene expression and function at numerous levels, such as RNA splicing, stability, translocation, and translation. There has been limited investigation into the connection between TNBC and m6A.
Materials and Methods
Breast cancer-related data were retrieved from the Cancer Genome Atlas (TCGA) database, and 116 triple-negative breast cancer cases were identified from the data. The GSE31519 dataset, which included 68 cases of TNBC, was obtained from the Gene Expression Omnibus (GEO) database. Survival analysis was used to determine the prognosis of distinct m6A types based on their m6A group, gene group, and m6A score. To investigate the potential mechanism, GO and KEGG analyses were performed on the differentially expressed genes.
Results
The expression of m6A-related genes and their impact on prognosis in TNBC patients were studied. According to the findings, m6A was crucial in determining the prognosis of TNBC patients, and the major m6A-linked genes in this process were YTHDF2, RBM15B, IGFBP3, and WTAP. By cluster analysis, the gene cluster and the m6A cluster were beneficial in predicting the prognosis of TNBC patients. The m6A score based on m6A and gene clusters was more effective in predicting the prognosis of TNBC patients. Furthermore, the tumor microenvironment may play an important role in the process of m6A, influencing TNBC prognosis.
Conclusion
N6-adenylic acid methylation (m6A) was important in altering the prognosis of TNBC patients, and the key m6A-associated genes in this process were YTHDF2, RBM15B, IGFBP3, and WTAP. Furthermore, the comprehensive typing based on m6A and gene clusters was useful in predicting TNBC patients' prognosis, showing potential as a meaningful evaluating tools for TNBC.
Hydro-chemical studies and assessment of trace elements and bacterial contamination of shallow groundwater of Oyo area, southwestern Nigeria
Authors: Akanbi Olanrewaju Akinfemiwa , Akinola Oluwagbemileke Samuel , Nwajei Jeremiah , Adegbite Joshua 
Published: August 2022

Abstract
Groundwater from various shallow hand-dug wells is the only reliable source of water supply in the ancient town of Oyo in southwestern Nigeria. The present work studied the water quality in twenty-five wells across this area by measuring the ancillary parameters, and analyzing the major and trace elements and taking the inventory of the wells as well as assessing the total coliform and Escherichia coliform in sampled water. The field measurement showed that the groundwater is slightly acidic with an average pH of 6.6 and fresh from the total dissolved solids values between 70 and 630 mg/L. From the average concentrations of major ions, the dominance order of the cations constituents in groundwater is in the order- Mg2+ > Ca2+ > K+ > Na+ and HCO- > CI- > NO3- > SO42- for anions. The ranges of trace elements concentrations were; Iron II- 0.01 – 0.06; Chromium VI: 0 - 0.34; manganese 0 – 1.2 in mg/L, while Copper was between 0.38 – 32.7; Cadmium 0.03 – 1.87; lead 0.02 – 3.57; Zinc 0.01 – 7.79; and Arsenic 0.05 – 7.35 in µg/L. The total coliform count ranges from 6 – 1860 cfu/100 mL while Escherichia coliform (E. coli) units ranges from nil in three wells representing 12% of the wells, 1 – 50 units (28%), 51 – 100 (20%), 101 – 500 (20%) and > 500 units (20%). The groundwater is grossly polluted with nitrate and coliform bacteria while four wells were found to be contaminated with manganese and chromium.
Can benefit equity alleviate health inequity? --Evidence from the reform of Health Insurance Programs in China
Authors: Li Rui , Wu Jing , Lu Jiaqian 
Published: August 2022

Abstract
The higher the socioeconomic status, the better the health status. Can the equity reform of health insurance alleviate the health inequality between different groups? Taking the provincial-level coordination reform of Chinese Urban Employee Basic Medical Insurance (UEBMI) as an example, the paper used the China Family Panel Studies (CFPS) data and relevant policy documents to examine the effect of equalized health insurance programs on health inequity by Recentered Influence Function (RIF). The empirical results showed that although the gap in medical reimbursement costs between participants of different socioeconomic status was narrowing after the provincial-level coordination reform, the health inequality among the insured population widened. The equalized health insurance reform can’t alleviate the health inequality brought about by socioeconomic status. This paper suggests that in the progress of promoting the provincial-level coordination reform of UEBMI, the protection of vulnerable groups should be strengthened to promote social equity.JEL codes: I10, I14, I30
FeOOH nanolayers decorated on oxygen-vacancy-containing BiVO 4 for efficient photoelectrochemical degradation of Rhodamine B
Authors: Ma Mingyang , Ruan Mengnan , Nie Weixing 
Published: August 2022

Abstract
The short carrier diffusion length, the trade-off between sufficient light absorption and efficient charge separation of BiVO4 photoelectrode often leads to poor photoelectrochemical (PEC) degradation performance. In this paper, we firstly designed a method of combining oxygen vacancies (Ov) with cocatalyst FeOOH for synergistic improve BiVO4 PEC degradation performance. After introducing Ov resulted from hydrothermal method, the photocurrent density value reaches 0.54 mA/cm2 at 1.23 V vs. RHE by the enhanced of carrier density and the broaden of light absorption, 1.38 times higher than that of pure BiVO4 (0.39 mA/cm2 at 1.23 V vs. RHE). As expected, the as-synthesized BiVO4-Ov/FeOOH exhibits enhanced stability and a remarkable photocurrent density of 1.18 mA cm2 at 1.23 V vs. RHE under AM 1.5G illumination. In addition, a significant PEC degradation performance of 85% were reached, which is about 1.7 times than BiVO4-Ov. The results demonstrate that the Ovs can be effectively preserved by the coating of a thin FeOOH layer, leading to a photoelectrode of enhanced photocurrent and stability.