bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profiles and transition of mental health problems among Chinese adolescents: The predictive role of friendship quality, parental autonomy support, and psychological control
Authors: Liu Xinyi , Cui Lijuan , Wu Hang , Liu Ben , Yang Ying 
Published: July 2022

AbstractIntroductionMental health problems have become a global crisis of the 21st century, with adolescence being a typical period of the outbreak of these problems. However, the profiles and transition of mental health problems in Chinese adolescents remain unclear. In addition, protective and risk factors that shape mental health problems require further clarification.MethodsWe measured depression, anxiety, and stress as indicators to identify the profiles and transition patterns of mental health problems among Chinese adolescents, as well as environment‐related predictors (i.e., friendship quality, parental psychological control, and autonomy support). A total of 722 participants (376 females;Mage = 15.21,SDage = 0.74) completed a set of questionnaires at two time points with a 1‐year interval (T1: November 2018; T2: November 2019).ResultsThe profile analysis revealed two groups:healthyandtroubled. The development of mental health problems included four trajectories: steady low, steady high, increasing, and decreasing. Results indicated that parental autonomy support and friendship quality exerted protective and buffering effects, whereas parental psychological control acted as a risk factor for mental health problem profiles. Furthermore, friendship quality had a unique predictive effect on the decreasing trajectory.ConclusionsThe profiles of mental health problems showed high concurrency of depression, anxiety, and stress symptoms, and developmental trajectories were largely stable over time. Friendship quality, parental autonomy support, and psychological control predicted the profiles of the mental health problems of Chinese adolescents, and only friendship quality predicted the transition from atroubledtohealthyprofile over 1 year.
A development of a graph‐based ensemble machine learning model for skin sensitization hazard and potency assessment
Authors: Jeon Byoungjun , Lim Min Hyuk , Choi Tae Hyun , Kang Byeong‐Cheol , Kim Sungwan 
Published: July 2022

AbstractMany defined approaches (DAs) for skin sensitization assessment based on the adverse outcome pathway (AOP) have been developed to replace animal testing because the European Union has banned animal testing for cosmetic ingredients. Several DAs have demonstrated that machine learning models are beneficial. In this study, we have developed an ensemble prediction model utilizing the graph convolutional network (GCN) and machine learning approach to assess skin sensitization. The model integrates in silico parameters and data from alternatives to animal testing of well‐defined AOP to improve DA predictivity. Multiple ensemble models were created using the probability produced by the GCN with six physicochemical properties, direct peptide reactivity assay, KeratinoSens™, and human cell line activation test (h‐CLAT), using a multilayer perceptron approach. Models were evaluated by predicting the testing set's human hazard class and three potency classes (strong, weak, and non‐sensitizer). When the GCN feature was used, 11 models out of 16 candidates showed the same or improved accuracy in the testing set. The ensemble model with the feature set of GCN, KeratinoSens™, and h‐CLAT produced the best results with an accuracy of 88% for assessing human hazards. The best three‐class potency model was created with the feature set of GCN and all three assays, resulting in 64% accuracy. These results from the ensemble approach indicate that the addition of the GCN feature could provide an improved predictivity of skin sensitization hazard and potency assessment.
CuO‐SiO2 based nanocomposites: Synthesis, characterization, photocatalytic, antileishmanial, and antioxidant studies
Authors: Yaseen Muhammad , Farooq Saima , Khan Abbas , Shah Nasrullah , Shah Luqman Ali , Bibi Shaista , Khan Imdad Ullah , Ahmad Sajjad 
Published: July 2022

AbstractThe abatement of toxic pollutants and drug‐resistant pathogens from water is one of the major environmental issues. In this work, we are reporting the preparation, detailed characterization and photocatalytic degradation, and biological activities of bimetallic oxides (CuO‐SiO2) nanocomposite. For this purpose, CuO, SiO2 nanoparticles, and CuO‐SiO2 nanocomposites were successfully synthesized through sol–gel process. The nanoparticles and nanocomposite were prepared by taking the Cu(NO3)2.3H2O as copper oxide precursor and TEOS as silica precursor using absolute ethanol and water as solvents The molar ratio of TEOS: Glycerol: H2O used was 1.5:1:6.6 respectively. The synthesized nanoparticles and nanocomposites were characterized by using Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier‐transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD) and Brunauer–Emmett–Teller (BET). The nanocomposites and individual nanoparticles were then applied as catalysts for their activities toward catalytic/photocatalytic degradation of crystal violet (CV) dye in an aqueous medium. The degradation progress was monitored by UV–Visible spectroscopic analysis. It was found that CuO‐SiO2 nanocomposite shows good photocatalytic activities toward the removal of crystal violet dye as compared to the catalytic activities of its individual constitutes. The antileishmanial and antioxidant activities results indicate that synthesized materials have promising biological attributes.
Extracellular vesicles and lipoproteins – Smart messengers of blood cells in the circulation
Authors: Nguyen Phuong H.D. , Le Anh Hong , Pek Jonetta Shi Qi , Pham Thach Tuan , Jayasinghe Migara Kavishka , Do Dang Vinh , Phung Cao Dai , Le Minh T.N. 
Published: July 2022

AbstractBlood cell‐derived extracellular vesicles (BCEVs) and lipoproteins are the major circulating nanoparticles in blood that play an important role in intercellular communication. They have attracted significant interest for clinical applications, given their endogenous characteristics which make them stable, biocompatible, well tolerated, and capable of permeating biological barriers efficiently. In this review, we describe the basic characteristics of BCEVs and lipoproteins and summarize their implications in both physiological and pathological processes. We also outline well accepted workflows for the isolation and characterization of these circulating nanoparticles. Importantly, we highlight the latest progress and challenges associated with the use of circulating nanoparticles as diagnostic biomarkers and therapeutic interventions in multiple diseases. We spotlight novel engineering approaches and designs to facilitate the development of these nanoparticles by enhancing their stability, targeting capability, and delivery efficiency. Therefore, the present work provides a comprehensive overview of composition, biogenesis, functions, and clinical translation of circulating nanoparticles from the bench to the bedside.
Cefotaxime resistance in bile samples is an independent predictor of deep infectious complications after pancreaticoduodenectomy in patients with endoprosthesis
Authors: Robin Fabien , Livin Marie , Bergeat Damien , Triki Haitham , Gaignard Elodie , Cailleaux Marine , Cattoir Vincent , Boudjema Karim , Tattevin Pierre , Sulpice Laurent 
Published: July 2022

AbstractBackgroundBacteriobilia may increase the rate of deep infectious complications (DIC) after pancreaticoduodenectomy. To better adjust prophylactic and empirical antibacterial treatment, we aimed to characterize bacteriobilia in patients with preoperative endoprosthesis, and its association with postoperative DIC.MethodsAll patients who underwent pancreaticoduodenectomy in our center between 2010 and 2019 were included. The association between microbiological findings from bile samples, and postoperative DIC was analyzed, and we compared microbiology data between 2010‐2014 and 2015‐2019 periods.ResultsWe enrolled 578 patients (median age 67 years [59‐72], 58.7% males), of whom 220 (38.1%) had preoperative biliary endoprosthesis, with 197 (89.5%) positive preoperative bile samples pathogens were Enterobacterales, enterococci, and Candida albicans. The incidence of DIC was similar in patients with or without endoprosthesis (20.4% vs 17.8%, P = .352). Bacterial isolates collected during 2015‐2019 were more resistant to cefotaxime than those recovered from 2010‐2014 (45.5% vs 25.5%, P = .009). The only independent risk factor for DIC in patients with endoprosthesis was cefotaxime resistance in bile (hazard ratio 3.027 [1.115‐8.216], P = .03).ConclusionsThe incidence of DIC is high after pancreaticoduodenectomy, with or without endoprosthesis, despite routine postoperative treatment. Cefotaxime resistance, the only independent predictor of DIC in patients with endoprosthesis, has increased over time. Hence, cefotaxime may no longer be an appropriate empirical treatment.
Trehalose‐6‐phosphate synthase influences polysaccharide synthesis and cell wall components in Ganoderma lucidum
Authors: Chen Juhong , Fan Junpei , Liu Weidong , Wang Zi , Ren Ang , Shi Liang 
Published: July 2022

AbstractTrehalose‐6‐phosphate synthase (TPS) is a key enzyme that participates in trehalose metabolism, which can synthesize trehalose in a two‐step pathway with trehalose phosphatase, but its role in fungi is rarely studied, especially in large basidiomycetes. In this study, the tps gene of Ganoderma lucidum was cloned and named as gltps. And gltps‐silenced strains were constructed by RNA interference. In this study, it is found that the extracellular polysaccharide content increased 1.6–2‐fold, but there was no significant change on intracellular polysaccharide content in gltps‐silenced strains compared with the wild‐type (WT) strain. Furthermore, the cell wall compositions of the gltps‐silenced strains were also altered, which showed that the chitin and β‐1,3‐glucan contents were significantly decreased. Compared with WT, the concentration of chitin decreased by 20%–50% and that of β‐1, 3‐glucan decreased by 15%–30%. The study found that the cells of gltps‐silenced strains were more sensitive to cell wall stress, which might be due to changes in the compounds and structure of the cell wall. These results showed that gltps had an important effect on carbohydrate metabolism of G. lucidum cells.
Interdecadal variation in atmospheric water vapour content over East Asia during winter and the relationship with autumn Arctic sea ice
Authors: He Wenyue , Sun Bo , Ma Jiehua , Wang Huijun 
Published: July 2022

AbstractThis study focused on the interdecadal variability in water vapour content (WVC) over East Asia during winter and its associated mechanisms. The interdecadal variation in the WVC over East Asia during winter was investigated using the empirical orthogonal function (EOF) method. The leading mode (EOF1, explained variance is 52.7%) of interdecadal variability in the WVC, which explains most of the interdecadal variation, exhibits a monopole pattern characterized by consistent WVC anomalies over East Asia. The mechanisms associated with the interdecadal variability in winter WVC over East Asia were further investigated, and it was found that the interdecadal variability in winter WVC over East Asia is modulated by three water vapour transport (WVT) branches from the eastern, western, and southern boundaries of East Asia that are associated with Eurasian winter circulation. Further analyses revealed that increased sea ice concentration over the Barents‐Kara Sea (SIC‐BKS) in October–November (ON) serves as the source of wave activity flux and stimulates a stationary wave train along the Eurasian continent to change the downstream atmospheric circulation. As a response, an abnormal cyclone, an abnormal anticyclone, and an abnormal cyclone are triggered over western Siberia, the northern area of East Asia, and the southern area of East Asia, which are together favourable for WVC sufficiency over East Asia. Sensitivity experiments by the Community Atmosphere Model, version 4 (CAM4) using sea ice forcing in the Barents Sea yield similar results to the observed data, further confirming our conclusion. Therefore, the SIC‐BKS in ON may be an important potential predictor for the interdecadal variability in winter WVC over East Asia.
Impact of starch–lipid complexes on oil absorption of starch and its mechanism
Authors: Luo Shunjing , Xiong Shaobai , Li Xianbao , Hu Xiuting , Ye Jiangping , Liu Chengmei 
Published: July 2022

AbstractBackgroundWorldwide, fried food has a huge demand and good development prospects. Low oil in foods is the standard that everyone is now pursuing for a healthy diet.ResultsThe oil absorption behavior of rice starch during frying was investigated in the presence or absence of fatty acids or fatty acid esters with different carbon chain lengths. The complex formed between starch and fatty acids or fatty acid esters was dependent on lipid chain length, which was confirmed by X‐ray diffraction and complexing index. The formation of starch–lipid complexes could significantly reduce the oil absorption of starch, and the complexes with higher complexing index had lower oil absorption. The starch–palmitic acid complex showed the lowest oil absorption after frying, which was 14.06 g per 100 g lower than that of gelatinized starch. This was attributed to the ability of the palmitic acid to increase the density of starch crystalline polymorphs as well as their ability to complex with the amylose spiral cavity.ConclusionThese results may be useful for development of healthier fried starch‐based foods with reduced oil contents. © 2022 Society of Chemical Industry.
Overexpression of circ PTK2 suppresses the progression of nonalcoholic fatty liver disease via the miR‐200c/SIK2/PI3K/Akt axis
Authors: Li Yong , Cen Chao‐Qun , Liu Bo , Zhou Lu , Huang Xiang‐Miao , Liu Geng‐Yan 
Published: July 2022

AbstractExcessive hepatic lipid accumulation is involved in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). A previous study showed that the circular RNA (circRNA) PTK2 was significantly downregulated in NAFLD mice. However, the detailed function of circ PTK2 in NAFLD remains unclear. A high‐fat diet (HFD) was used to establish a mouse model of NAFLD, and free fatty acid (FFA) treatment was used to establish an in vitro model of NAFLD. Oil red O staining was used to evaluate lipid accumulation. The pathological changes in mice were observed by HE staining. Western blotting and RT–qPCR were applied to assess protein and mRNA levels, respectively. A dual luciferase reporter assay and RIP were used to explore the relationship among circ PTK2, miR‐200c and SIK2. Circ PTK2 and SIK2 were downregulated and miR‐200c was upregulated in NAFLD. Upregulation of circ PTK2 reversed lipid accumulation in FFA‐treated HepG2 cells. Moreover, circ PTK2 bound to miR‐200c, and SIK2 was identified as the direct target of miR‐200c. Moreover, the miR‐200c inhibitor‐induced decrease in lipid accumulation was reversed by SIK2 knockdown. Furthermore, the impact of circ PTK2 overexpression on PI3K/Akt signaling was partially reversed by SIK2 silencing. Circ PTK2 overexpression alleviates NAFLD development via the miR‐200c/SIK2/PI3K/Akt axis. Thus, our work might provide new methods for NAFLD treatment.
Association of patient characteristics, ultrasound features, and molecular testing with malignancy risk in Bethesda III–V thyroid nodules
Authors: Talmor Guy , Badash Ido , Zhou Sheng , Kim Yun Ji , Kokot Niels C. , Hsueh Wayne , Chambers Tamara 
Published: July 2022

AbstractObjectiveTo evaluate the role of patient characteristics, ultrasound findings, and molecular testing in predicting risk of malignancy in Bethesda III, IV, and V thyroid nodules.DesignSingle institution retrospective review of 230 patients with Bethesda III, IV, and V cytopathology undergoing thyroidectomy between 2009 and 2018.SettingPrivate and public tertiary urban university hospitals at the same academic institution.Subjects and methodsPatients who underwent thyroidectomy with Bethesda III, IV, and V nodules were included. Patient demographics, presence of underlying thyroid disease, nodule size, sonographic features, gene expression results, and surgical procedure were documented. Correlation between these variables and final histopathologic diagnosis of malignancy was analyzed.ResultsThe 230 patients (103 Bethesda III, 64 Bethesda IV, and 63 Bethesda V) were included for analysis. Bethesda III nodules harbored malignancy in 26.2% of cases compared with 26.6% of Bethesda IV nodules and 82.5% of Bethesda V nodules. On multivariate analysis, age was inversely correlated with a diagnosis of malignancy (OR: 0.98, 95% confidence interval [CI]: 0.96–0.99, p = .03) Although the presence of microcalcifications was positively associated with cancer (OR: 2.31, CI: 1.24–4.29, p = .008) The co‐occurrence of microcalcifications and irregular margins was associated with a higher odds of malignancy (OR: 4.42, 95% CI: 1.32–14.93, p = .016), whereas the combination of microcalcifications, irregular margins, and hypoechogenicity was associated with the greatest cancer risk (OR: 5.52, 95% CI: 1.12–27.78, p = .036).ConclusionsThe presence of microcalcifications in thyroid nodules categorized as Bethesda III–V is an independent risk factor for malignancy. The combination of microcalcifications, irregular margins, and hypoechogenicity is associated with a greater malignancy risk in nodules indeterminate for thyroid cancer on cytopathology.
High‐Performance Poly(biphenyl acetylpyridine) and Poly(ether ketone cardo) Blend Membranes for High‐Temperature Polymer Electrolyte Membrane Fuel Cells
Authors: Jin Yaping , Wang Ting , Tang Weiqin , Yu Na , Yang Jingshuai 
Published: July 2022

AbstractDeveloping high‐temperature polymer electrolyte membranes with sufficient performance and competitive cost is a major scientific checkpoint for promoting the popularization of fuel cells. Herein, poly(biphenyl acetylpyridine) (PBAP) is synthesized by a facile one‐pot Friedel‐Crafts polymerization between 4‐acetylpyridine and biphenyl. The PBAP has excellent solubility in polar solvents, while its membrane shows a significantly high phosphoric acid (PA) absorption capacity due to the presence of amounts of pyridine groups. However, the pure PBAP membrane is dissolved in 85 wt.% PA solution. In order to solve excessive expansion and poor mechanical stability, a series of PBAP blend membranes are fabricated by mixing PBAP with commercial engineering thermoplastic of poly(ether ketone cardo) (PEKC). Blending PEKC significantly improves the dimensional and mechanical stabilities. In particular, blend membranes exhibit remarkable chemical stability as documented by Fenton test, while membranes after Fenton test display slightly higher acid uptakes and conductivities. As a result, the PBAP‐50wt.%PEKC/170%PA membrane shows a conductivity of 0.051 S cm−1 at 160 °C and mechanical strength of 8.3 MPa at 25 °C. The peak power density at 160 °C of the H2‐O2 single cell with above membrane is 632 mW cm−2, indicating the potential of PBAP‐x%PEKC/PA membranes for fuel cells.
TWNK in Parkinson's Disease: A Movement Disorder and Mitochondrial Disease Center Perspective Study
Authors: Percetti Marco , Franco Giulia , Monfrini Edoardo , Caporali Leonardo , Minardi Raffaella , La Morgia Chiara , Valentino Maria Lucia , Liguori Rocco , Palmieri Ilaria , Ottaviani Donatella , Vizziello Maria , Ronchi Dario , Di Berardino Federica , Cocco Antoniangela , Macao Bertil , Falkenberg Maria , Comi Giacomo Pietro , Albanese Alberto , Giometto Bruno , Valente Enza Maria , Carelli Valerio , Di Fonzo Alessio 
Published: July 2022

AbstractBackgroundParkinsonian features have been described in patients harboring variants in nuclear genes encoding for proteins involved in mitochondrial DNA maintenance, such as TWNK.ObjectivesThe aim was to screen for TWNK variants in an Italian cohort of Parkinson's disease (PD) patients and to assess the occurrence of parkinsonism in patients presenting with TWNK‐related autosomal dominant progressive external ophthalmoplegia (TWNK‐adPEO).MethodsGenomic DNA of 263 consecutively collected PD patients who underwent diagnostic genetic testing was analyzed with a targeted custom gene panel including TWNK, as well as genes causative of monogenic PD. Genetic and clinical data of 18 TWNK‐adPEO patients with parkinsonism were retrospectively analyzed.ResultsSix of 263 PD patients (2%), presenting either with isolated PD (n = 4) or in combination with bilateral ptosis (n = 2), carried TWNK likely pathogenic variants. Among 18 TWNK‐adPEO patients, 5 (28%) had parkinsonism.ConclusionsWe show candidate TWNK variants occurring in PD without PEO. This finding will require further confirmatory studies. © 2022 Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson Movement Disorder Society.
Analysis of L1CAM gene mutation and imaging appearance in three Chinese families with L1 syndrome: Three case reports
Authors: Gao Shanshan , Zhao Xuechao , Zhao Ganye , Dai Peng , Kong Xiangdong 
Published: July 2022

AbstractBackgroundThe molecular mutations of the L1CAM gene and the imaging appearances of four fetuses with L1 syndrome from three independent Chinese families with a history of hydrocephalus were reported in this study. Two of the three are novel L1CAM variants.MethodsResults of clinical and imaging examinations of three Chinese families were collected. Fetal samples were collected by puncture, genomic DNA was extracted, whole‐exome sequencing was performed, and the L1CAM gene mutation sites were verified by PCR and Sanger sequencing.ResultsIn this case report, we described the imaging appearance and investigated the mutations of the L1CAM gene in three Chinese families with a history of L1 syndrome; these included two nonsense mutations (c.262C>T and c.261C>G) and one splice‐site mutation (c.524‐1G>A). Two of these three are novel L1CAM variants: c.262C>T and c.261C>G. The results of the sonographic images of the affected fetuses showed severe hydrocephalus. Bilateral lateral ventricles were dilated in the fetuses with c.262C>T and c.261C>G mutations. The left ventricle was about 14 mm wide and the right was about 14 mm in the fetus with c.262C>T mutation. The left ventricle was about 24.9 mm wide and the right was about 23.9 mm in the fetus with c.261C>G mutation. The ultrasound examination of the fetus with c.524‐1G>A mutation showed that the third ventricle (7.5 mm wide) was raised, and the fourth ventricle was communicated with the cisterna magna. The parents requested termination of the above pregnancy.ConclusionThe current study emphasizes the importance of combining family history, prenatal ultrasonography, and L1CAM mutation testing positive for the diagnosis of the L1 syndrome.