bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mammoth Fauna Remains From Late Pleistocene Deposits of the Dmitry Laptev Strait South Coast (Northern Yakutia, Russia)
Authors: Kuznetsova Tatiana V. , Wetterich Sebastian , Matthes Heidrun , Tumskoy Vladimir E. , Schirrmeister Lutz 
Published: June 2022

The Yedoma Ice Complex in northern Yakutia provides perfect preservation conditions for frozen remains of vertebrate animals. Even complete mummified specimens of the late Pleistocene Beringian Mammoth fauna such as woolly mammoth, woolly rhinoceros, horse, and bison are occasionally found in permafrost deposits across eastern Siberia, i.e., in West Beringia, although bones are much more commonly found. The present study characterizes mammal bones from late Pleistocene and Holocene permafrost deposits exposed on the Oyogos Yar coast, part of the southern shore of the Dmitry Laptev Strait that connects the Laptev and East Siberian seas. The study applies a method to characterize fossil bone samples by the location of their discovery and by the accuracy of their relation to a depositional horizon. We analyzed a total of 38 finite radiocarbon ages of bone material from mammoth, horse, and musk ox, spanning from about 48.8 to 4.5 ka BP and including both our own data and data from the literature, in addition to previous publications that reported numerous bones with infinite ages from the Oyogos Yar coast. The distribution of bones and tooth along the coastal permafrost exposure is not uniform; it depends upon whether the material was found in situ, on thermo-terraces, or on the shore. The overall bone collection consists of 13 species of which Mammuthus primigenius (woolly mammoth, 41%), Bison priscus (bison, 19%), Equus ex gr., caballus (horse, 19%), and Rangifer tarandus (reindeer, 16%) predominate. The fossil bone species distribution is similar to those of other prominent Yedoma outcrops in the region, i.e., on Bykovsky Peninsula and on Bol’shoy Lyakhovsky Island. Correlation analysis shows that the Oyogos Yar bone sampling sites of different geomorphological settings are similar to each other but not to all sampling sites within the other two locations on Bykovsky Peninsula and on Bol’shoy Lykahovsky Island. High similarities in terms of correlation coefficients between specific sampling sites are often not represented in the cluster analysis.
The Alteration of m6A Modification at the Transcriptome-Wide Level in Human Villi During Spontaneous Abortion in the First Trimester
Authors: She Jiajie , Tan Kaifen , Liu Jie , Cao Shuo , Li Zengguang , Peng You , Xiao Zhuoyu , Diao Ruiying , Wang Liping 
Published: June 2022

A growing number of studies have demonstrated that N6 methyladenine (m6A) acts as an important role in the pathogenesis of reproductive diseases. Therefore, it is essential to profile the genome-wide m6A modifications such as in spontaneous abortion. In this study, due to the trace of human villi during early pregnancy, we performed high-throughput sequencing in villous tissues from spontaneous abortion (SA group) and controls with induced abortion (normal group) in the first trimester. Based on meRIP-seq data, 18,568 m6A peaks were identified. These m6A peaks were mainly located in the coding region near the stop codon and were mainly characterized by AUGGAC and UGGACG motif. Compared with normal group, the SA group had 2,159 significantly upregulated m6A peaks and 281 downregulated m6A peaks. Biological function analyses revealed that differential m6A-modified genes were mainly involved in the Hippo and Wnt signaling pathways. Based on the conjoint analysis of meRIP-seq and RNA-seq data, we identified thirty-five genes with differentially methylated m6A peaks and synchronously differential expression. And these genes were mainly involved in the Wnt signaling pathway, phosphatase activity regulation, protein phosphatase inhibitor activity, and transcription inhibitor activity. This study is the first to profile the transcriptome-wide m6A methylome in spontaneous abortion during early pregnancy, which provide novel insights into the pathogenesis and treatment of spontaneous abortion in the first trimester.
Construction of Immune-Related ceRNA Network in Dilated Cardiomyopathy: Based on Sex Differences
Authors: Liu Chang , Liu Jian , Wu Daihong , Luo Shaoling , Li Weijie , Chen Lushan , Liu Zhen , Yu Bingbo 
Published: June 2022

Background: Immune targeted therapy has become an attractive therapeutic approach for patients with dilated cardiomyopathy (DCM) recently. Genetic predisposition and gender play a critical role in immune-related responses of DCM. This study aimed to perform a bioinformatics analysis of molecular differences between male and female samples and identify immune-related ceRNA network in DCM.Methods: The gene expression microarray and clinical features dataset of GSE19303 was downloaded from the GEO. The raw data were preprocessed, followed by identification of differentially expressed genes (DEGs) between male and female DCM samples. Crucial functions and pathway enrichment analysis of DEGs were investigated through GO analysis and KEGG pathway analysis, respectively. A lncRNA–miRNA–mRNA network was constructed and a central module was extracted from the ceRNA network.Results: Compared with the female group, the male group benefits more from IA/IgG immunotherapy. Male patients of DCM had a significant positive correlation with the abundance of inflammatory cells (B cells, memory B cells, CD8+ Tem cells, and NK cells). Sex difference DEGs had a widespread impact on the signaling transduction, transcriptional regulation, and metabolism in DCM. Subsequently, we constructed an immune-related ceRNA network based on sex differences in DCM, including five lncRNAs, six miRNAs, and 29 mRNAs. Furthermore, we extracted a central module from the ceRNA network, including two lncRNAs (XIST and LINC00632), three miRNAs (miR-1-3p, miR-17-5p, and miR-22-3p), and six mRNAs (CBL, CXCL12, ESR1, IGF1R, IL6ST, and STC1). Among these DEGs, CBL, CXCL12, and IL6ST expression was considered to be associated with inflammatory cell infiltration in DCM.Conclusions: The identified ceRNA network and their enriched pathways may provide genetic insights into the phenotypic diversity of female and male patients with DCM and may provide a basis for development of sex-related individualization of immunotherapy.
TMED2/9/10 Serve as Biomarkers for Poor Prognosis in Head and Neck Squamous Carcinoma
Authors: Gao Wen , Zhang Zhe-Wen , Wang Hong-Yi , Li Xin-Di , Peng Wei-Ting , Guan Hao-Yu , Liao Yu-Xuan , Liu An 
Published: June 2022

Background: Head and neck squamous carcinoma (HNSC) is one of the most common malignant tumors with high incidence and poor prognosis. Transmembrane emp24 structural domain (TMED) proteins are involved in protein transport and vesicle budding processes, which have implicated various malignancies’ progression. However, the roles of TMEDs in HNSC, especially in terms of development and prognosis, have not been fully elucidated.Methods: We applied TIMER 2.0, UALCAN, GEPIA 2, Kaplan-Meier plotter, GEO, The Human Protein Atlas (HPA), cBioPortal, Linkedomics, Metascape, GRNdb, STRING, and Cytoscape to investigate the roles of TMED family members in HNSC.Results: Compared with normal tissues, the mRNA expression levels of TMED1/2/4/5/7/8/9/10 were significantly increased in the TCGA HNSC dataset. And we combined GEPIA 2 and Kaplan-Meier Plotter to select TMED2/9/10 with prognostic value. Then we detected the levels of mRNA in the GEO HNSC database and the protein expression in HPA. It was found that the mRNA and protein expression levels of TMED2/9/10 were increased in HNSC. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that TMED2/9/10 and their co-expressed genes promoted the malignant behavior of tumors by participating in biological processes such as intracellular transferase complex, protein transport, focal adhesion, intracellular protein processing. Single-cell analysis and immune infiltration analysis suggested that immune responses of cancer-associated fibroblasts and endothelial cells might be associated with prognosis. Finally, the transcription factors-genes network and protein-protein functional interaction network pointed to genes such as X-box binding protein 1 (XBP1) and TMED7, which might cooperate with TMED2/9/10 to change the progression of HNSC.Conclusions: Our study implied that TMED2/9/10 and related genes mightjointly affect the prognosis of HNSC, providing specific clues for further experimental research, personalized diagnosis strategies, and targeted clinical therapy for HNSC.
Two-Year Event-Free Survival Prediction in DLBCL Patients Based on In Vivo Radiomics and Clinical Parameters
Authors: Ritter Zsombor , Papp László , Zámbó Katalin , Tóth Zoltán , Dezső Dániel , Veres Dániel Sándor , Máthé Domokos , Budán Ferenc , Karádi Éva , Balikó Anett , Pajor László , Szomor Árpád , Schmidt Erzsébet , Alizadeh Hussain 
Published: June 2022

PurposeFor the identification of high-risk patients in diffuse large B-cell lymphoma (DLBCL), we investigated the prognostic significance of in vivo radiomics derived from baseline [18F]FDG PET/CT and clinical parameters.MethodsPre-treatment [18F]FDG PET/CT scans of 85 patients diagnosed with DLBCL were assessed. The scans were carried out in two clinical centers. Two-year event-free survival (EFS) was defined. After delineation of lymphoma lesions, conventional PET parameters and in vivo radiomics were extracted. For 2-year EFS prognosis assessment, the Center 1 dataset was utilized as the training set and underwent automated machine learning analysis. The dataset of Center 2 was utilized as an independent test set to validate the established predictive model built by the dataset of Center 1.ResultsThe automated machine learning analysis of the Center 1 dataset revealed that the most important features for building 2-year EFS are as follows: max diameter, neighbor gray tone difference matrix (NGTDM) busyness, total lesion glycolysis, total metabolic tumor volume, and NGTDM coarseness. The predictive model built on the Center 1 dataset yielded 79% sensitivity, 83% specificity, 69% positive predictive value, 89% negative predictive value, and 0.85 AUC by evaluating the Center 2 dataset.ConclusionBased on our dual-center retrospective analysis, predicting 2-year EFS built on imaging features is feasible by utilizing high-performance automated machine learning.
Serum Total Bile Acids in Relation to Gastrointestinal Cancer Risk: A Retrospective Study
Authors: Li Songbo , Qu Xiaodong , Zhang Luyao , Wang Na , Chen Min , Zhao Xingyu , Wang Jie , Lv Huanhuan , Qi Ying , Zhang Lifeng , Liu Junye , Shi Yongquan 
Published: June 2022

BackgroundBile acids (BAs) have been proposed to promote gastrointestinal cells carcinogenesis. However, studies on serum total bile acid (TBA) levels and gastrointestinal cancers (GICs) risk are rare.MethodsWe conducted a retrospective case–control study from 2015 to 2019 at the First Affiliated Hospital of Air Force Military Medical University, in which 4,256 GICs cases and 1,333 controls were recruited. Patients’ demographic, clinical and laboratory data were collected. The odds ratios (ORs) with 95% confidence intervals (CIs) were estimated using binary logistic regression models.ResultsPositive associations were observed between serum TBA levels and risks of esophageal cancer (EC), gastric cancer (GC) and colorectal cancer (CRC). Overall, ORs of EC, GC and CRC risk rose with the TBA levels increasing. After adjustment for potential confounders, the OR of TBA-positive for EC risk was 4.89 (95% CI: 3.20-7.49), followed by GC (OR: 3.92, 95% CI: 2.53-6.08), and CRC (OR: 3.32, 95% CI: 2.04-5.11). Patients aged 60 years or older have a higher risk of GICs, especially for EC patients. Males are associated with a higher risk of GC, while females are associated with a higher risk of CRC. Preoperative serum TBA positive and negative was significantly different in the presence or absence of hematogenous metastasis among EC patients (P=0.014), and lymph node metastasis among GC patients (P=0.018).ConclusionsThis retrospective study showed positive associations between serum TBA level and GICs risk, and a higher serum TBA level constitutes a risk factor for GICs.
Karyotype of the Blastocoel Fluid Derived by Laser-Assisted Hatching Demonstrates a Low Agreement With the Trophectoderm
Authors: Wang Liang , Pang Wenjuan , Zhang Yi , Hao Min , Liu Yan , Wang Xiang , Sun Ningxia 
Published: June 2022

ObjectiveThe aim of this study is to compare the amplification efficiency and the genomic profiles of blastocoel fluid (BF) derived by laser-assisted hatching and trophectoderm (TE) cells derived from the same blastocyst.MethodsFifty-four fresh blastocysts underwent shrinkage by laser-assisted hatching, and each BF sample was collected individually. BF and TE cells were retrieved from each blastocyst for chromosome analysis through multiple annealing and looping-based amplification cycles (MALBAC) and next-generation sequencing (NGS).ResultsFifty-four BF samples and 32 TE samples were retrieved for this study. Out of the 54 BF samples, only 35 provided reliable NGS data for comprehensive chromosome analysis (64.8%), while all 32 TE samples did (100%). Finally, there were 23 pairs of BF and TE samples from the same blastocyst. Only 17.4% of the BF-DNA karyotypes were completely agreeable with the TE samples (4/23).ConclusionBlastocoel fluid derived by laser-assisted hatching is easy to operate, and BF-DNA can be successfully amplified and subjected to NGS. Due to the low amplification efficiency and increased discordance with TE, BF does not adequately represent the status of the rest of the blastocyst. The use of BF as a single source of DNA for preimplantation genetic screening (PGS) is not yet advised.
Small Molecule RPI-194 Stabilizes Activated Troponin to Increase the Calcium Sensitivity of Striated Muscle Contraction
Authors: Mahmud Zabed , Tikunova Svetlana , Belevych Natalya , Wagg Cory S. , Zhabyeyev Pavel , Liu Philip B. , Rasicci David V. , Yengo Christopher M. , Oudit Gavin Y. , Lopaschuk Gary D. , Reiser Peter J. , Davis Jonathan P. , Hwang Peter M. 
Published: June 2022

Small molecule cardiac troponin activators could potentially enhance cardiac muscle contraction in the treatment of systolic heart failure. We designed a small molecule, RPI-194, to bind cardiac/slow skeletal muscle troponin (Cardiac muscle and slow skeletal muscle share a common isoform of the troponin C subunit.) Using solution NMR and stopped flow fluorescence spectroscopy, we determined that RPI-194 binds to cardiac troponin with a dissociation constant KD of 6–24 μM, stabilizing the activated complex between troponin C and the switch region of troponin I. The interaction between RPI-194 and troponin C is weak (KD 311 μM) in the absence of the switch region. RPI-194 acts as a calcium sensitizer, shifting the pCa50 of isometric contraction from 6.28 to 6.99 in mouse slow skeletal muscle fibers and from 5.68 to 5.96 in skinned cardiac trabeculae at 100 μM concentration. There is also some cross-reactivity with fast skeletal muscle fibers (pCa50 increases from 6.27 to 6.52). In the slack test performed on the same skinned skeletal muscle fibers, RPI-194 slowed the velocity of unloaded shortening at saturating calcium concentrations, suggesting that it slows the rate of actin-myosin cross-bridge cycling under these conditions. However, RPI-194 had no effect on the ATPase activity of purified actin-myosin. In isolated unloaded mouse cardiomyocytes, RPI-194 markedly decreased the velocity and amplitude of contractions. In contrast, cardiac function was preserved in mouse isolated perfused working hearts. In summary, the novel troponin activator RPI-194 acts as a calcium sensitizer in all striated muscle types. Surprisingly, it also slows the velocity of unloaded contraction, but the cause and significance of this is uncertain at this time. RPI-194 represents a new class of non-specific troponin activator that could potentially be used either to enhance cardiac muscle contractility in the setting of systolic heart failure or to enhance skeletal muscle contraction in neuromuscular disorders.
Effects of Parecoxib Sodium Application Combined with Enhanced Recovery After Surgery Nursing on Inflammatory Factors and Knee Joint Function in Elderly Patients After Total Knee Arthroplasty
Authors: Deng Liqiong , Tan Liping 
Published: June 2022

ObjectivesTo study the effect of parecoxib sodium (PS) application, combined with enhanced recovery after surgery (ERAS) nursing, on inflammation and knee joint function in elderly patients after total knee arthroplasty (TKA).MethodsIn this prospective cross-sectional study, we recruited 120 elderly patients treated with TKA who were randomly divided into two groups, the combine group and the control group, with 60 patients in each group. Patients in the control group received ERAS nursing and normal saline, and the patients in the combine group received ERAS nursing and PS. At different times after surgery, we compared the hemoglobin (Hb), complete white blood cell count (WBC), erythrocyte sedimentation rate (ESR), and serum IL-1β, TNF-α, and IL-6, and recovery time for different ranges of joint motion and the knee joint function HSS (hospital for special surgery scale) score between the two groups.ResultsOn the third and seventh postoperative days, the levels of Hb in the patients of the combine group were significantly lower than those in the control group (p &lt; 0.05), while the levels of WBC, ESR, serum IL-1β, TNF-α, and IL-6 in the patients of the combine group were all significantly lower than those in the control group (p &lt; 0.05). Compared with the patients in the control group, the recovery time for 30, 60, 90, and 120 angles of joint motion in patients of the combine group was significantly decreased (p &lt; 0.05), and the HSS score of patients in the combine group was significantly higher than that in the control group on the first, third, and sixth postoperative months (p &lt; 0.05).ConclusionElderly TKA patients who received PS application, combined with ERAS nursing, had lower inflammation in peripheral blood 2 weeks after operation and faster postoperative recovery of knee joint function.
EFICÁCIA DO CALDO DE FUMO (NICOTIANA TABACUM) NO CONTROLE DO MENACANTHUS STRAMINEUS (PHTHIRAPTERA: MENOPONIDAE) EM GALINHAS POEDEIRAS NO MUNICÍPIO DE SALINAS, MINAS GERAIS
Authors: Dias de Oliveira Natan , Dantas de Medeiros Fonseca Vitor , Ribeiro Aquino Sâny , Alves Nascimento Nágila , Gomes Sousa Elber , Paulino da Cruz Vieira Vanessa 
Published: June 2022

Em granjas de postura, infestações por ectoparasitas são um problema que gera gastos e diminui a produção dos animais, pelo aumento do estresse. O piolho Menacanthus stramineus é apontado como um dos principais parasitas na avicultura comercial. Há um interesse crescente no uso de abordagens de controle de parasitos na produção de aves minimizando o uso de pesticidas sintéticos tradicionais. Objetivou-se avaliar a eficácia do caldo de fumo (Nicotiana tabacum) no controle do Menacanthus stramineus (Phthiraptera: Menoponidae) em galinhas poedeiras no município de Salinas, Minas Gerais. Para isso, 30 galinhas poedeiras naturalmente infestadas por piolhos, oriundas dos Setor de Zootecnia I do Instituto Federal do Norte de Minas Gerais - Campus Salinas (IFNMG), foram selecionadas aleatoriamente e divididas em três grupos, mantidos em galpões distintos e separados: Grupo I- Tratado com caldo de fumo, conforme Sagrilo e colaboradores (2007); Grupo II- Tratado com Colosso Pulverização®, diluídos 11ml em nove litros; Grupo III- Controle, tratado com água, sendo todos os grupos submetidos ao tratamento por imersão em balde com nove litros de solução. Espécimes de piolhos foram coletados manualmente através de inspeção visual, juntamente com penas contendo ovos em sua base e encaminhados ao Laboratório de Parasitologia Veterinária (LPV) do IFNMG, para realização do diagnóstico parasitológico através de chaves de identificação. Os espécimes foram clarificados em hidróxido de potássio a 10% e observados em microscopia óptica nas objetivas de 4x e 10x. Os ovos foram observados em microscópio estereoscópico, no aumento de 4x. Todas as galinhas foram diagnosticadas com infestação por ovos e adultos de M. stramineus. Para o banho de imersão, as aves foram seguradas pelo pescoço e pelas asas, mergulhando todo seu corpo em balde plástico. Sete dias após a realização dos tratamentos, as aves foram novamente examinadas em uma inspeção visual pelos mesmos examinadores. O Grupo I não apresentou ovos e nem adultos de M. stramineus em nenhuma ave, revelando 100% de eficácia. O Grupo II não apresentou adultos, mas foram observados ovos aderidos à base das penas, friáveis ao toque, em nove aves. Posteriormente, verificou-se que se tratava de cascas de ovos vazias. E, o Grupo III estava ainda intensamente parasitado por ovos e adultos de M. stramineus. Conclui-se que o caldo de fumo (Nicotiana tabacum) preparado conforme descrito na metodologia do presente trabalho, é eficaz no controle do piolho Menacanthus stramineus (Phthiraptera: Menoponidae) em galinhas poedeiras no município de Salinas, Minas Gerais.
ПРОФЕСІЙНІ ЦІННОСТІ ВИКЛАДАЧА ЗАКЛАДУ ВИЩОЇ ОСВІТИ ЯК ЧИННИК ЙОГО ОСОБИСТІСНОГО ЗРОСТАННЯ
Authors: Мачинська Наталія 
Published: June 2022

Стаття присвячена аналізу теоретичних положень проблеми цінностей педагога вищої школи в аспекті його особистісного зростання. У статті зазначено, що феномен професійних цінностей є багатоструктурним новоутовутворенням, що формується у процесі професійної підготовки майбутнього викладача ще в умовах закладу вищої освіти. Показано, що професійні цінності одночасно можна розглядати як критеріальні аспекти професійного становлення майбутнього ввикладача, так і вагому складову його професійного зростання упродовж життя.
Автором доведено, що для позначення поняття «особистісні цінності» дослідники використовують поняття «усвідомленість», «рефлексивність»; особистісні цінності розглядають як складну ієрархічну модель, яка локалізується на перетині мотиваційно-потребнісної сфери особистості; роль ціннісних орієнтацій визначають провідними життєвими цілями людини. Зазначено, що «цінність» найчастіше науковці трактують як «направленість», «смисл», «значення», «мотив», «потреба», «настановлення».
У статті акцентовано увагу на теорії цінностей Шварца, запропоновано п’ять основних характеристик цінностей; показано, що цінності мають важливе еволюційне значення у процесі розвитку індивіда, адже формуються на основі базових людських потреб. Зазначено: всі цінності формуються на основі задоволеності чи не задоволеності певних потреб; автор теорії пропонує структуру цінностей відповідно до основних потреб людини.