bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of the Delaying Gratification Inventory in Japanese (DGI‐J)
Authors: Goto Takayuki , Yanaoka Kaichi , Ozaki Yuka , Hoerger Michael 
Published: February 2025

AbstractWe often face situations requiring us to exert self‐control, wherein we set goals and invest plenty of effort in achieving them, but are also disturbed by urges to give in to temptations. Since self‐control is associated with outcomes across various domains, it is important to assess individual domain‐specific differences in self‐control to further understand how it develops and contributes to successful outcomes. The present study aimed to develop and validate the Japanese version of the Delaying Gratification Inventory, a well‐developed measure for assessing one's ability to delay gratification in five domains: food, physical pleasures, social interactions, money, and achievement. We created the Delaying Gratification Inventory in Japanese (DGI‐J) using back translation. We conducted surveys to test its validity using two discrete samples: crowd‐workers and university students. Our results showed that the DGI‐J is a valid measure for assessing self‐control in the abovementioned domains, except for the internal consistency for the Physical subscale. We also created a 10‐item short form of the DGI‐J from an item‐response theory perspective and tested its validity. We discuss how the DGI‐J can contribute to the literature on self‐control.
Effects of dietary citric acid, lactic acid, and potassium sorbate mixture on growth performance and intestinal immunological parameters in common carp (Cyprinus carpio) juveniles
Authors: Hoseini Seyyed Morteza , Al Sulivany Basim S. A. , Afzali‐Kordmahalleh Alireza , Abdollahpour Hamed , Rajabiesterabadi Hamid , Yousefi Morteza 
Published: February 2025

AbstractAcidifiers serve as effective feed additives that enhance fish growth and welfare. This study investigates the effects of a mixture of lactic acid, citric acid, and potassium sorbate (denoted as OA, in a 1:1:1 ratio) on common carp (Cyprinus carpio) for the first time. A total of 240 fish, each weighing approximately 18.5 grams, were randomly assigned to four treatment groups, each consisting of four replicates containing 15 fish. The fish were fed diets supplemented with varying levels of OA: 0 g/kg (Control), 2.5 g/kg (A‐2.5), 5 g/kg (A‐5), and 10 g/kg (A‐10) for a duration of 56 days. Growth performance, calcium and phosphorus levels in vertebrae and muscle, intestinal antioxidant status, bacterial populations, gene expressions, as well as immunological parameters in skin mucus and blood plasma were analyzed at the end of the experiment. The results indicated a significant (p < 0.0001) increase in the abundance of Lactobacillus sp. in the OA‐treated groups compared to the Control group. Conversely, the intestinal populations of Vibrio sp. (p < 0.0001) and Aeromonas sp. (p = 0.010) significantly decreased in the OA‐treated groups, particularly in the A‐10 treatment. Moreover, significant (p < 0.0001) up‐regulations were observed in the expression of intestinal tumor necrosis factor‐alpha (tnf‐a), interleukin‐1 beta (il‐1b), heat shock protein 70 (hsp70), and mucin‐2 (muc‐2) across all OA‐treated groups when compared to the Control group. All OA‐treated groups, especially the A‐5 treatment, exhibited significant (p < 0.0001) increases in the expression of intestinal lysozyme (lys), mucin‐5 (muc‐5), and beta‐defensin (b‐def) relative to the Control group. Furthermore, intestinal levels of reduced glutathione (GSH) significantly increased (p = 0.0003), while malondialdehyde (MDA) levels decreased (p = 0.002) in all OA‐treated groups, particularly in the A‐5 treatment. Notably, plasma lysozyme (p = 0.002), alternative complement (AC; p = 0.022), skin mucus protease (p = 0.011), lysozyme (p = 0.002), and alkaline phosphatase (ALP; p = 0.001) showed significant increases in the A‐5 and/or A‐10 treatments compared to the Control group. Dietary supplementation with OA significantly enhanced muscle and vertebral ash, phosphorus, and calcium levels, particularly in the A‐5 treatment. Additionally, growth performance and feed efficiency significantly improved in the A‐5 treatment when compared to the Control group. In conclusion, dietary supplementation with 5 g/kg of OA proved beneficial for common carp by enhancing intestinal health, humoral and mucosal immune responses, calcium and phosphorus retention, as well as overall growth performance.
NrdR in Streptococcus and Listeria spp.: DNA Helix Phase Dependence of the Bacterial Ribonucleotide Reductase Repressor
Authors: Shahid Saher , Balka Mateusz , Lundin Daniel , Daley Daniel O. , Sjöberg Britt‐Marie , Rozman Grinberg Inna 
Published: February 2025

ABSTRACTNrdR is a universal transcriptional repressor of bacterial genes coding for ribonucleotide reductases (RNRs), essential enzymes that provide DNA building blocks in all living cells. Despite its bacterial prevalence, the NrdR mechanism has been scarcely studied. We report the biochemical, biophysical, and bioinformatical characterization of NrdR and its binding sites from two major bacterial pathogens of the phylum Bacillota Listeria monocytogenes and Streptococcus pneumoniae. NrdR consists of a Zn‐ribbon domain followed by an ATP‐cone domain. We show that it forms tetramers that bind to DNA when loaded with ATP and dATP, but if loaded with only ATP, NrdR forms various oligomeric complexes unable to bind DNA. The DNA‐binding site in L. monocytogenes is a pair of NrdR boxes separated by 15–16 bp, whereas in S. pneumoniae, the NrdR boxes are separated by unusually long spacers of 25–26 bp. This observation triggered a comprehensive binding study of four NrdRs from L. monocytogenes, S. pneumoniae, Escherichia coli, and Streptomyces coelicolor to a series of dsDNA fragments where the NrdR boxes were separated by 12–27 bp. The in vitro results were confirmed in vivo in E. coli and revealed that NrdR binds most efficiently when there is an integer number of DNA turns between the center of the two NrdR boxes. The study facilitates the prediction of NrdR binding sites in bacterial genomes and suggests that the NrdR mechanism is conserved throughout the bacterial domain. It sheds light on RNR regulation in Listeria and Streptococcus, and since NrdR does not occur in eukaryotes, opens a way to the development of novel antibiotics.
MdVQ37 Negatively Regulates Apple Resistance to Valsa Canker via SA‐Dependent and SA‐Independent Pathways
Authors: Duan Dingyue , Zheng Wenqian , Shi Mingrui , Yi Ran , Dong Qinglong , Yang Jie , Ma Fengwang , Mao Ke 
Published: February 2025

ABSTRACTApple Valsa canker is one of the major fungal diseases adversely affecting the apple industry. Valine‐glutamine motif‐containing proteins (VQs) are a kind of plant transcription regulation cofactor, which are involved in regulating the growth and development of plants and their defence responses. Yet little is known about the role of VQs in the biotic stress response of woody plants, especially in apple trees. Recently, we identified MdVQ37 as a negative regulator of apple resistance to Glomerella leaf spot by inhibiting MdWRKY100‐mediated salicylic acid (SA) accumulation via protein interaction. Here we found that MdVQ37 expression was induced significantly by Valsa mali infection. Overexpressing MdVQ37 in apple increased its susceptibility to V. mali, accompanied by a reduction in H2O2 accumulation along with the activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), chitinase and β‐1,3‐glucanase. Meanwhile, MdVQ37 overexpression increased the expression of two SA catabolic genes, MdS5H1 and MdS5H2, resulting in a greater accumulation of the SA metabolite 2,5‐dihydroxybenzoic acid (2,5‐DHBA), but a decreased SA content and less signalling in transgenic plants. MdPER3, a peroxidase‐encoding gene, was identified as a direct target of MdWRKY100. Analysis of transcriptional regulation activity showed that MdVQ37 inhibited the binding and transcriptional ability of MdWRKY100 on the MdPER3 promoter, thereby reducing antioxidant enzyme activity and weakening the Valsa canker resistance. Overall, our results show that MdVQ37 negatively regulates apple defence against V. mali through both SA‐dependent and SA‐independent pathways. Our findings provide novel insight into the mechanisms by which the VQ–WRKY complex modulates plant defence responses against pathogens.
Making Sense of Nationality: On the Attempt of Signifying Identity
Authors: Kögel Johannes 
Published: February 2025

ABSTRACTReferences to nationality can serve various purposes beyond signifying belonging or membership to a polity. Tracking the logic of nationality, using an empirical example, combines the concept of schismogenesis with the notions of the floating signifier and suture. Signifiers set purely by means of contrast to others denote a chain of signifiers without an anchoring point, leaving it in a floating state. Nationality seems to occupy such a point, suturing the chain of particular national signifiers. However, given that nationality can be distinguished as content and form, or identity and membership, it appears that one component becomes ephemeral when the other is articulated. Nationality, therefore, can be seen as an attempt to fix identity or essentialize meaning and additionally operates as an internally floating signifier. Nationalism is defined as the re‐entry of nationality as a form on the side of its content, essentializing belonging without putting its identity into discussion.
General‐purpose genotypes and evolution of higher plasticity in clonality underlie knotweed invasion
Authors: Wang Shengyu , Liao Zhi‐Yong , Cao Peipei , Schmid Marc W. , Zhang Lei , Bi Jingwen , Endriss Stacy B. , Zhao Yujie , Parepa Madalin , Hu Wenyi , Akamine Hikaru , Wu Jihua , Ju Rui‐Ting , Bossdorf Oliver , Richards Christina L. , Li Bo 
Published: February 2025

Summary
Many widespread invasive plant species express high phenotypic variation across novel environments, providing a unique opportunity to examine ecological and evolutionary dynamics under global change. However, studies often lack information about the origin of introduced populations, limiting our understanding of post‐introduction evolution.
We assessed the responses of Reynoutria japonica from 128 populations spanning latitudinal transects in the native (China and Japan), and introduced (North America and Europe) ranges when grown in two common gardens.
Plants from introduced populations differed in almost all traits from those from Chinese populations, but were similar to plants from the putative origin in Japan. Compared to Chinese populations, North American, European and Japanese populations expressed lower trait values and plasticity in most traits. However, plants from both introduced and Japanese populations expressed higher clonality and plasticity in clonality than plants from Chinese populations. Further, introduced populations expressed higher plasticity in clonality but lower plasticity in basal diameter compared to Japanese populations.
Our findings emphasize the potential role of clonality and plasticity in clonality for invasion success. In addition, our study highlights the importance of comparisons to source populations within the native range to identify evolutionary responses of introduced plants to novel environments.
Associations between psychological inflexibility processes, pre‐sleep arousal and sleep quality
Authors: Mastin‐Purcell Lisa , Richdale Amanda L. , Lawson Lauren P. , Morris Eric M. J. 
Published: February 2025

AbstractObjectivesPsychological inflexibility, the model of psychopathology underlying Acceptance and Commitment Therapy (ACT), has been linked to a broad range of psychological problems, but its link with sleep quality is not well understood. This study aimed to identify relationships between psychological inflexibility processes (cognitive fusion, experiential avoidance, limited perspective‐taking, lack of present moment awareness and inaction) and sleep quality, investigating pre‐sleep arousal and anxiety symptomatology as mechanisms mediating these relationships.DesignA correlational, cross‐sectional design was used to test two statistical models.MethodsParticipants (N = 704) from a general population sample completed an online survey, reporting on sleep quality, pre‐sleep arousal, anxiety and measures of psychological inflexibility. Data were analysed using correlations and path analyses.ResultsAll psychological inflexibility processes were moderate to strongly correlated with sleep quality. Path analysis showed cognitive fusion, lack of present moment awareness and experiential avoidance, mediated by pre‐sleep cognitive and somatic arousal, explained 49% of the variance in sleep quality (Model 1). Cognitive fusion via pre‐sleep cognitive arousal had the largest effect. Anxiety preceding pre‐sleep arousal (Model 2) explained no additional variance, and model fit was poorer than Model 1.ConclusionsThese findings highlight the role of psychological inflexibility processes in disrupting the de‐arousal process needed for healthy sleep, supporting evidence for ACT as a treatment for sleep disturbance.
OsMAPKKK5 affects brassinosteroid signal transduction via phosphorylating OsBSK1–1 and regulates rice plant architecture and yield
Authors: Yan Peiwen , Wang Ying , Cui Jinhao , Liu Mingyu , Zhu Yu , Ma Fuying , Liu Yahui , Lan Dengyong , Dong Shiqing , Hu Zejun , Niu Fuan , Liu Yang , Zhang Xinwei , He Shicong , Hu Jian , Yuan Xinyu , Li Yizhen , Yang Jinshui , Cao Liming , Luo Xiaojin 
Published: February 2025

SummaryImproving plant architecture and increasing yields are the main goals of rice breeders. However, yield is a complex trait influenced by many yield‐related traits. Identifying and characterizing important genes in the coordinated network regulating complex rice traits and their interactions is conducive to cultivating high‐yielding rice varieties. In this study, we determined that the interaction between mitogen‐activated protein kinase kinase kinase5 (OsMAPKKK5) and brassinosteroid‐signalling kinase1–1 (OsBSK1–1) regulates yield‐related traits in rice. Specifically, OsMAPKKK5 phosphorylates OsBSK1–1, which enhances the interaction between these two proteins, but adversely affects the OsBSK1–1–OsBRI1 (BR insensitive1) and OsBSK1–1–OsPPKL1 (protein phosphatase with two Kelch‐like domains) interactions. Additionally, OsMAPKKK5 disrupts brassinosteroid signal transduction, which prevents OsBZR1 (brassinazole‐resistant1) from efficiently entering the nucleus, thereby negatively modulating its function as a transcription factor regulating downstream effector genes, ultimately adversely affecting plant architecture and yield. This study revealed the relationship between the MAPK cascade and the regulatory effects of brassinosteroid on the rice grain yield involves OsMAPKKK5 and OsBSK1–1. The study data may be important for future investigations on the rice yield‐regulating molecular network.
LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen
Authors: Meng Jiaxin , Wang Han , Liu Yixin , Wang Yu , Li Houhua 
Published: February 2025

ABSTRACTLow‐nitrogen (LN) conditions typically promote anthocyanin biosynthesis in plants. Although previous studies primarily explored structural genes and transcription factors involved in anthocyanin biosynthesis, the role of long noncoding RNAs (lncRNAs) in this process under LN conditions remains less understood. This study identified LNC159c as a potential regulator of anthocyanin biosynthesis, with its expression notably downregulated under LN conditions. LNC159c functions as the host gene of miR159c, which regulates its expression. A dual‐luciferase reporter assay and GFP signal detection demonstrated that miR159c inhibited MsMYB10 expression through base complementary pairing. Yeast one‐hybrid (Y1H) and dual‐LUC reporter assays jointly verified that MsMYB10 binds to the F3'H promoter. Stable overexpression in apple calli and transient overexpression in apple fruit peels and M. spectabilis leaves showed that LNC159c overexpression promoted miR159c expression, while inhibiting MsMYB10 expression and anthocyanin biosynthesis. MsMYB10 overexpression promoted anthocyanin biosynthesis, whereas the opposite results were observed in MsMYB10‐silenced tissues. Thus, under LN conditions, reduced LNC159c expression limits miR159c production, relieving MsMYB10 inhibition and enhancing anthocyanin accumulation. These findings highlight the regulatory role of lncRNAs in anthocyanin biosynthesis under LN conditions.
Salicylic Acid Cooperates With Lignin and Sucrose Signals to Alleviate Waxy Maize Leaf Senescence Under Heat Stress
Authors: Wang Zitao , Guo Jian , Luo Wenxuan , Niu Shiduo , Qu Lingling , Li Jing , Chen Yanping , Li Guanghao , Yang Huan , Lu Dalei 
Published: February 2025

ABSTRACTLeaf senescence induced by high temperature (HT) has become a primary factor limiting maize yield, particularly during the filling stage. Exogenous salicylic acid (SA) has emerged as an effective strategy to mitigate leaf senescence and HT‐induced damage, though its underlying mechanisms remain unclear. This study investigated the regulatory mechanism of SA application on waxy maize subjected to HT during the early filling stage. Compared to HT alone, exogenous SA alleviated the inhibition of photosynthesis and oxidative damage by enhancing the activities of enzymes involved in photosynthesis and antioxidant system and modulating phytohormone metabolism and signal transduction pathways, thereby reducing leaf senescence and mitigating yield loss under HT. Transcriptomic and metabolomic analyses showed that HT downregulated most genes involved in the starch and sucrose metabolism pathway in leaves but promoted soluble sugar accumulation, which represents a plant strategy to cope with HT. Conversely, exogenous SA reversed this change and further enhanced soluble sugar accumulation in leaves. SA also regulated sugar metabolism by inhibiting trehalose‐6‐phosphate synthesis and activating SnRK1 to resist HT. Furthermore, SA stimulated lignin biosynthesis through the phenylpropanoid pathway, ensuring cell membrane integrity under HT. The relationship between SA signalling and plant heat tolerance was validated using a maize SA synthesis‐synthetic mutant.
GRAIN SIZE ONCHROMOSOME 2 orchestrates phytohormone, sugar signaling and energy metabolism to confer thermal resistance in rice
Authors: Li Guangyan , Wang Huanran , Li Hubo , Feng Baohua , Fu Weimeng , Ma Jiaying , Li Juncai , Wu Zhihai , Islam Md Rezaul , Chen Tingting , Zhang Hongcheng , Wei Haiyan , Tao Longxing , Fu Guanfu 
Published: February 2025

AbstractGRAIN SIZE ON CHROMOSOME 2 (GS2) has been reported to enhance rice grain yield and confer tolerance to cold, drought, and salt stress, but its function in heat tolerance of rice remains undocumented. This study aimed to investigate whether GS2 could enhance thermal tolerance by subjecting rice seedlings of Huazhan (HZ) and its near‐isogenic line (HZ−GS2) to heat stress. HZ−GS2 plants exhibited less damage compared to HZ plants under heat stress. Transcriptome revealed the involvement of phytohormones, sugar signaling, and energy metabolism in the mechanism by which GS2 influences heat tolerance. Under heat stress, HZ−GS2 plants showed higher increases or lower decreases in glucose, gibberellins (GAs), salicylic acid (SA), indoleacetic acid (IAA), adenosine triphosphate (ATP), energy charge, as well as the activities of hexokinase, NADH dehydrogenase, cytochrome oxidase, ATP synthase, and ATPase. Exogenous GA3 enhanced heat tolerance in rice by increasing energy charge, ATPase, activities of complex V and hexokinase. Additionally, glucose, sucrose, 3‐aminobenzamide (3‐ab), and Na2SO3 conferred heat tolerance in rice plants. Importantly, a significant increase in Fv/Fm was observed in plants treated with a combination of GA3, glucose, and 3‐ab, compared to those sprayed alone. Thus, GS2 coordinates GA3, hexokinase, and energy metabolism to improve energy status, thereby enhancing heat tolerance in rice plants.
Hormonal changes associated with arbuscular mycorrhizal fungi indicate defense‐like alterations in virus‐stressed grapevine
Authors: Gaši Emanuel , Likar Matevž , Arbona Vicent , González‐Guzmán Miguel , Hančević Katarina , Balestrini Raffaella , Čarija Mate , Regvar Marjana , Gambino Giorgio , Sillo Fabiano , Radić Tomislav 
Published: February 2025

AbstractGrapevine is an economically important crop, affected by major production losses due to high virus prevalence. Arbuscular mycorrhizal fungi (AMF) can reduce the impact of plant biotic stresses. However, hormonal response to the simultaneous presence of viruses and AMF remains largely unknown. In this study, we explored the potential of AMF to modify the grapevine's defense response to compatible virus infections. We used GRSPaV, GLRaV‐3, and GPGV as infectious viral agents, separately or in different combinations. Two AMF inoculums were tested for their bioprotective abilities, RHIZ (Rhizophagus irregularis) and MIX (R. irregularis, Funneliformis mosseae, F. caledonium). Generally, MIX induced stronger physiological responses than RHIZ inoculum, especially during the earlier phase of symbiosis. The main findings were connected to the hormonal profile of the grapevine infected by all three viruses and inoculated with MIX. In particular, salicylic acid (SA) and abscisic acid (ABA) concentrations were induced five and fifteen months post AMF inoculation, respectively. Expressions of VvNCED1 and VvBG1 were up‐regulated in uninoculated grapevines, indicating slower induction of stress response mechanisms. Parameters related to plant vigour and growth were induced in grapevine at both time points, regardless of the virus combination. In conclusion, the defense‐like response induced by AMF in grapevines infected with multiple viruses is characterized by the induction of ABA and SA, accompanied by a consistent enhancement of vigor parameters. This study confirms AMF symbiosis as a potentially promising additional tool for combating viral diseases in vineyards.