bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of central autonomic nervous system dysfunction in Takotsubo syndrome: a systematic review
Authors: Brazdil Vojtech , Kala Petr , Hudec Martin , Poloczek Martin , Kanovsky Jan , Stipal Roman , Jerabek Petr , Bocek Otakar , Pail Martin , Brazdil Milan 
Published: January 2022

Abstract
Introduction
Takotsubo syndrome (TTS), also known as stress cardiomyopathy or “broken heart” syndrome, is a mysterious condition that often mimics an acute myocardial infarction. Both are characterized by left ventricular systolic dysfunction. However, this dysfunction is reversible in the majority of TTS patients.

Purpose
Recent studies surprisingly demonstrated that TTS, initially perceived as a benign condition, has a long-term prognosis akin to myocardial infarction. Therefore, the health consequences and societal impact of TTS are not trivial. The pathophysiological mechanisms of TTS are not yet completely understood. In the last decade, attention has been increasingly focused on the putative role of the central nervous system in the pathogenesis of TTS.

Conclusion
In this review, we aim to summarize the state of the art in the field of the brain–heart axis, regional structural and functional brain abnormalities, and connectivity aberrancies in TTS.
Towards an integrated neonatal brain and cardiac examination capability at 7 T: electromagnetic field simulations and early phantom experiments using an 8-channel dipole array
Authors: Clément Jérémie , Tomi-Tricot Raphaël , Malik Shaihan J. , Webb Andrew , Hajnal Joseph V. , Ipek Özlem 
Published: January 2022

AbstractObjectiveNeonatal brain and cardiac imaging would benefit from the increased signal-to-noise ratio levels at 7 T compared to lower field. Optimal performance might be achieved using purpose designed RF coil arrays. In this study, we introduce an 8-channel dipole array and investigate, using simulations, its RF performances for neonatal applications at 7 T.MethodsThe 8-channel dipole array was designed and evaluated for neonatal brain/cardiac configurations in terms of SAR efficiency (ratio between transmit-field and maximum specific-absorption-rate level) using adjusted dielectric properties for neonate. A birdcage coil operating in circularly polarized mode was simulated for comparison. Validation of the simulation model was performed on phantom for the coil array.ResultsThe 8-channel dipole array demonstrated up to 46% higher SAR efficiency levels compared to the birdcage coil in neonatal configurations, as the specific-absorption-rate levels were alleviated. An averaged normalized root-mean-square-error of 6.7% was found between measured and simulated transmit field maps on phantom.ConclusionThe 8-channel dipole array design integrated for neonatal brain and cardiac MR was successfully demonstrated, in simulation with coverage of the baby and increased SAR efficiency levels compared to the birdcage. We conclude that the 8Tx-dipole array promises safe operating procedures for MR imaging of neonatal brain and heart at 7 T.
Correlation between TXNRD1/HO-1 expression and response to neoadjuvant chemoradiation therapy in patients with esophageal squamous cell carcinoma
Authors: Akaishi Ryujiro , Fujishima Fumiyoshi , Ishida Hirotaka , Tsunokake Junichi , Yamauchi Takuro , Gokon Yusuke , Ueki Shunsuke , Fukutomi Toshiaki , Okamoto Hiroshi , Takaya Kai , Sato Chiaki , Taniyama Yusuke , Nakamura Tomohiro , Nakaya Naoki , Kamei Takashi , Sasano Hironobu 
Published: January 2022

Abstract
Background
Thioredoxin reductase 1 (TXNRD1) and heme oxygenase-1 (HO-1) are both involved in the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway and play key roles in antioxidant responses. In patients with esophageal squamous cell carcinoma (ESCC), the correlation between the expression of these two proteins and the therapeutic response to neoadjuvant chemoradiation therapy (NACRT), as well as the difference in their expression after chemoradiotherapy, remains unknown.

Methods
Proteins involved in the Nrf2 pathway were immunolocalized in carcinoma cells in ESCC patients on NACRT with 5-fluorouracil and cisplatin, followed by esophagectomy. The 8-hydroxydeoxyguanosine (8-OHdG) levels were used to quantify reactive oxygen species. The changes in immunoreactivity before and after NACRT (Δ) were assessed.

Results
Tumor reduction following NACRT was significantly attenuated in pre-therapeutic biopsy specimens associated with high HO-1 status. TXNRD1Δ, HO-1Δ, and 8-OHdGΔ were significantly different in the ineffective and effective groups. The overall survival was significantly lower in high Nrf2 and TXNRD1 groups. In addition, high TXNRD1 expression was an independent prognostic factor in the multivariate analysis of overall survival.

Conclusions
The study findings indicate that HO-1 status in pre-therapeutic biopsy specimens could predict response to NACRT, and TXNRD1 status could predict overall survival of ESCC patients.
PHD2 deletion in endothelial or arterial smooth muscle cells reveals vascular cell type-specific responses in pulmonary hypertension and fibrosis
Authors: Elamaa Harri , Kaakinen Mika , Nätynki Marjut , Szabo Zoltan , Ronkainen Veli-Pekka , Äijälä Ville , Mäki Joni M. , Kerkelä Risto , Myllyharju Johanna , Eklund Lauri 
Published: January 2022

AbstractHypoxia plays an important regulatory role in the vasculature to adjust blood flow to meet metabolic requirements. At the level of gene transcription, the responses are mediated by hypoxia-inducible factor (HIF) the stability of which is controlled by the HIF prolyl 4-hydroxylase-2 (PHD2). In the lungs hypoxia results in vasoconstriction, however, the pathophysiological relevance of PHD2 in the major arterial cell types; endothelial cells (ECs) and arterial smooth muscle cells (aSMCs) in the adult vasculature is incompletely characterized. Here, we investigated PHD2-dependent vascular homeostasis utilizing inducible deletions of PHD2 either in ECs (Phd2∆ECi) or in aSMCs (Phd2∆aSMC). Cardiovascular function and lung pathologies were studied using echocardiography, Doppler ultrasonography, intraventricular pressure measurement, histological, ultrastructural, and transcriptional methods. Cell intrinsic responses were investigated in hypoxia and in conditions mimicking hypertension-induced hemodynamic stress. Phd2∆ECi resulted in progressive pulmonary disease characterized by a thickened respiratory basement membrane (BM), alveolar fibrosis, increased pulmonary artery pressure, and adaptive hypertrophy of the right ventricle (RV). A low oxygen environment resulted in alterations in cultured ECs similar to those in Phd2∆ECi mice, involving BM components and vascular tone regulators favoring the contraction of SMCs. In contrast, Phd2∆aSMC resulted in elevated RV pressure without alterations in vascular tone regulators. Mechanistically, PHD2 inhibition in aSMCs involved actin polymerization -related tension development via activated cofilin. The results also indicated that hemodynamic stress, rather than PHD2-dependent hypoxia response alone, potentiates structural remodeling of the extracellular matrix in the pulmonary microvasculature and respiratory failure.
The 2020 national seismic hazard model for the United Kingdom
Authors: Mosca I. , Sargeant S. , Baptie B. , Musson R. M. W. , Pharaoh T. C. 
Published: January 2022

AbstractWe present updated seismic hazard maps for the United Kingdom (UK) intended for use with the National Annex for the revised edition of Eurocode 8. The last national maps for the UK were produced by Musson and Sargeant (Eurocode 8 seismic hazard zoning maps for the UK. British Geological Survey Report CR/07/125, United Kingdom, 2007). The updated model uses an up-to-date earthquake catalogue for the British Isles, for which the completeness periods have been reassessed, and a modified source model. The hazard model also incorporates some advances in ground motion modelling since 2007, including host-to-target adjustments for the ground motion models selected in the logic tree. For the first time, the new maps are provided for not only peak ground acceleration (PGA) but also spectral acceleration at 0.2 s (SA0.2s) and 1.0 s for 5% damping on rock (time-averaged shear wave velocity for the top 30 m Vs30 ≥ 800 m/s) and four return periods, including 475 and 2475 years. The hazard in most of the UK is generally low and increases slightly in North Wales, the England–Wales border region, and western Scotland. A similar spatial variation is observed for PGA and SA0.2sbut the effects are more pronounced for SA0.2s. Hazard curves, uniform hazard spectra, and disaggregation analysis are calculated for selected sites. The new hazard maps are compared with the previous 2007 national maps and the 2013 European hazard maps (Woessner et al. in Bull Earthq Eng 13:3553–3596, 2015). There is a slight increase in PGA from the 2007 maps to this work; whereas the hazard in the updated maps is lower than indicated by the European maps.
The Role of Moral Foundations, Anticipated Guilt and Personal Responsibility in Predicting Anti-consumption for Environmental Reasons
Authors: Culiberg Barbara , Cho Hichang , Kos Koklic Mateja , Zabkar Vesna 
Published: January 2022

AbstractIn response to the growing importance of environmental issues, more and more consumers are turning to anti-consumption by reducing, rejecting, or avoiding consumption. Covering the intersection of sustainable consumption and anti-consumption, previous studies relied on socio-cognitive models to explain this decision. In order to extend their findings, we consider the moral and emotional perspectives to examine reducing consumption for environmental reasons in a particular context, i.e. air travel. It is against this backdrop that we propose a conceptual model that includes moral foundations as the main antecedent, followed by anticipated guilt and personal responsibility, while intention to reduce consumption (i.e. air travel) for environmental reasons, positive word of mouth about reducing air travel (WOM) and environmental activism represent the outcomes. The proposed model is tested on a sample of 511 respondents from a UK online consumer panel. Our results confirm the importance of moral foundations, anticipated guilt and personal responsibility and their interplay in the prediction of intention to reduce consumption for environmental reasons. Anticipated guilt influences WOM, while personal responsibility influences activism. In addition, intentions to reduce consumption for environmental reasons have a positive impact on WOM and environmental activism. There are several implications for public policy makers and NGOs that fight against climate change that derive from these findings, as well as research opportunities for academics interested in this topic.
Mobility of kinetochore proteins measured by FRAP analysis in living cells
Authors: Watanabe Reito , Hirano Yasuhiro , Hara Masatoshi , Hiraoka Yasushi , Fukagawa Tatsuo 
Published: January 2022

AbstractThe kinetochore is essential for faithful chromosome segregation during mitosis and is assembled through dynamic processes involving numerous kinetochore proteins. Various experimental strategies have been used to understand kinetochore assembly processes. Fluorescence recovery after photobleaching (FRAP) analysis is also a useful strategy for revealing the dynamics of kinetochore assembly. In this study, we introduced fluorescence protein-tagged kinetochore protein cDNAs into each endogenous locus and performed FRAP analyses in chicken DT40 cells. Centromeric protein (CENP)-C was highly mobile in interphase, but immobile during mitosis. CENP-C mutants lacking the CENP-A-binding domain became mobile during mitosis. In contrast to CENP-C, CENP-T and CENP-H were immobile during both interphase and mitosis. The mobility of Dsn1, which is a component of the Mis12 complex and directly binds to CENP-C, depended on CENP-C mobility during mitosis. Thus, our FRAP assays provide dynamic aspects of how the kinetochore is assembled.
Bidirectional Relationship Between Family Accommodation and Youth Anxiety During Cognitive-Behavioral Treatment
Authors: Bertelsen Thomas B. , Himle Joeseph A. , Håland Åshild Tellefsen 
Published: January 2022

AbstractFamily accommodation is associated with an increase in anxiety and has recently received attention as a target for intervention for youth anxiety. Existing theories posit that the increase in family accommodation increases youth anxiety and can attenuate the effect of psychotherapy. However, the directionality between family accommodation and youth anxiety has not been investigated. A cross-lagged cross-panel design was used to assess accommodation and anxiety for 10 sessions for 73 youths with an anxiety disorder, who were receiving cognitive-behavioral therapy. The analysis revealed a bidirectional relationship, such that to some extent previous session family accommodation increased youth anxiety symptoms (β = 0.11, 95% CI [0.06, 0.17]), but to an even greater extent previous session youth-rated anxiety symptoms increased family accommodation (β = 0.23, 95% CI [0.08, 0.38]). Family accommodation is an important target for reducing youth anxiety but should be addressed simultaneously as interventions directly targeting youth anxiety.
Efficacy of apatinib combined with FOLFIRI in the first-line treatment of patients with metastatic colorectal cancer
Authors: Rong Xuetong , Liu Haiyi , Yu Hongmei , Zhao Jian , Wang Jie , Wang Yusheng 
Published: January 2022

SummaryObjective. To evaluate the efficacy and safety of apatinib combined with FOLFIRI in the first-line treatment of advanced metastatic colorectal cancer (mCRC) and explore potential factors of efficacy. Methods. Twenty mCRC patients treated at Affiliated Cancer Hospital of Shanxi Medical University from March 2017 to March 2019 were included according to the enrolment criteria. They provided informed consent and were treated with apatinib combined with FOLFIRI according to the scheduled regimen until disease progression or unacceptable toxicity occurred. The primary endpoint was OS. The secondary endpoints included PFS, ORR, DCRand safety. OS and PFS were calculated using Kaplan–Meier curves. Univariate and multivariate Cox regression analyses were used to evaluate independent prognostic factors of OS and PFS. R was used to determine cut-off values for biochemical indicators. Forest maps were drawn for Cox univariate results and the relationships between NLR and ECOG, which were significant in univariate analysis, and OS were represented by Kaplan–Meier curves. Results. The median OS and PFS were 16.135 months (95% CI: 9.211–22.929) and 6 months (95% CI: 5.425–6.525). Multivariate Cox analysis showed that NLR and CEA were independent prognostic factors. The most common grade 3–4 adverse events were hypertension, diarrhoea, increased alkaline phosphatase, decreased leukocytes and decreased neutrophils. Conclusion. Apatinib combined with FOLFIRI for the first-line treatment of advanced unresectable mCRC showed good efficacy and safety. The baseline NLR was predictive of efficacy, and a low baseline NLR (HR: 0.2895, P = 0.0084) was associated with improved OS.Clinical Research Registration Number: ChiCTR1800015308.
Has the European Union entered a bioeconomy transition? Combining an output-based approach with a shift-share analysis
Authors: Ronzon Tévécia , Iost Susanne , Philippidis George 
Published: January 2022

AbstractThe bioeconomy is a collective of activities charged with the production of biologically renewable resources or ‘biomass’ (e.g. agriculture, forestry), its diverse application (e.g. food, textiles, construction, chemicals) and subsequent reuse (e.g. compositing, waste management). Since the European Union (EU) launched its bioeconomy strategy in 2012, further bioeconomy policy initiatives have proliferated at regional, national and pan-European levels. Moreover, the EU Green Deal announced in 2019 targets a transition towards a low-carbon sustainable model of growth, food and energy security, biodiversity and natural resource management, where it is envisaged that the bioeconomy will play a key role. Despite a paucity of available data, the surge in policy interest has triggered the need for evidence-based monitoring of bioeconomy sectors and the efficient tailoring of policy support. Thus, on a Member State (MS) basis for the period 2008–2017, we (1) adopt an ‘output-based’ approach to construct a panel data of performance indicators and (2) characterise the sources of growth and transitional stage of the bioeconomy. Results reveal that the bioeconomy has maintained its relative importance within the total EU27 economy. At the EU level, agriculture and the food industry have played a key role in driving a transition in the primary and industrial bioeconomy sectors due to their significant labour productivity-enhancing impact. Four Northern MS exhibit a bioeconomy transition by modernising their bioeconomy activities and operating structural changes. Other Northern and Western EU MS are still in the early stages of a transition, whilst in Eastern and Central Europe, such a transition remains elusive.
Third-party vocal intervention in the proboscis monkey (Nasalis larvatus)
Authors: Scheumann Marina , Röper Kathrin M. , Nathan Senthilvel K. S. S. , Goossens Benoit 
Published: January 2022

AbstractVocal intervention is a triadic social interaction, where a third party responds vocally to a conflict between group members, minimizing the costs of aggression in response to the intervention. Because there is little information on vocal third-party intervention in nonhuman mammals, we investigated whether adult male proboscis monkeys use the bray vocalization as a vocal third-party intervention signal to intervene in intragroup conflicts. First, we audio-recorded 1,811 vocalizations from 17 free-ranging proboscis monkey groups in the Lower Kinabatangan Wildlife Sanctuary, analyzing 378 vocal responses of the adult male to agonistic vocal exchanges (shrieks) of group members. Second, we video- and audio-recorded five habituated groups in the Labuk Bay Proboscis Monkey Sanctuary investigating the context of these vocalizations and the conflict dyads evoking vocal support. We found that adult males of one-male/multifemale groups mainly uttered bray vocalizations, whereas females, immatures, and infants uttered shrieks in intragroup conflicts or in response to other animal species. The adult male uttered significantly more often brays after agonistic shrieks than expected based on the overall occurrence of brays. Brays ended 65% of agonistic conflicts, which were accompanied by vocalizations of the conflict partners and occurred more often after conflicts between females than between offspring. This suggests that the bray functions as a vocal third-party intervention signal for intragroup conflict resolution. We suggest that living in the high canopies of the tropical rainforest might restrict direct access to conflict partners and prevent physical intervention, favoring the evolution of the bray as a third-party vocal intervention signal.
G-protein coupled receptor 34 regulates the proliferation and growth of LS174T cells through differential expression of PI3K subunits and PTEN
Authors: Zuo Bo , Wu Na , Yang Shen , Zhong Zhaohui , Li Mei , Yu Xin , Liu Yulan , Yu Weidong 
Published: January 2022

Abstract
Purpose
G-protein coupled receptor (GPR 34) has been found to play important roles in some cancers and regulates the proliferation, apoptosis, and migration of these cancer cells. However, the mechanisms underlying how GPR34 functions to regulate growth and proliferation of colorectal cancer cells remains to be clarified.

Methods
We employed stable GPR34 knockdown LS174T cell models, GPR34 Mab blocking, a CCK-8 kit, and a colony formation assay to characterize the effect of GPR34 on the proliferation of LS174T in vitro and xenograft tumor growth in vivo. The mRNA level of GPR34 was detected by RT-PCR in tumor tissues and adjacent normal tissues from 34 CRC patients.

Results
Based on RT-PCR results, GPR34 exhibited high level in tumor samples compared with adjacent normal samples. Increased expression of GPR34 is more associated with poor prognosis of CRC as shown in The Cancer Genome Atlas (TCGA) dataset by Kaplan–Meier survival analysis. Furthermore, we showed that GPR34 knockdown inhibited the proliferation of LS174T colon cancer cells and related xenograft tumor growth. Searching for the distinct molecular mechanism, we identified several contributors to proliferation of LS174T colon cancer cells: PI3K subunits/PTEN, PDK1/AKT, and Src/Raf/Ras/ERK. GPR34 knockdown inhibited the proliferation of LS174T cells by upregulating expression of PTEN, and downregulating expression of PI3K subunits p110-beta.

Conclusion
Our findings provide direct evidence that GPR34 regulates the proliferation of LS174T cells and the growth of LS174T tumor xenografts by regulating different pathways. High expression of GPR34 mRNA could then be used to predict poor prognosis of CRC.
Predicting the impact of online news articles – is information necessary?
Authors: Preiss Judita 
Published: January 2022

AbstractWe exploit the Twitter platform to create a dataset of news articles derived from tweets concerning COVID-19, and use the associated tweets to define a number of popularity measures. The focus on (potentially) biomedical news articles allows the quantity of biomedically valid information (as extracted by biomedical relation extraction) to be included in the list of explored features. Aside from forming part of a systematic correlation exploration, the features – ranging from the semantic relations through readability measures to the article’s digital content – are used within a number of machine learning classifier and regression algorithms. Unsurprisingly, the results support that for more complex articles (as determined by a readability measure) more sophisticated syntactic structure may be expected. A weak correlation is found with information within an article suggesting that other factors, such as numbers of videos, have a notable impact on the popularity of a news article. The best popularity prediction performance is obtained using a random forest machine learning algorithm, and the feature describing the quantity of biomedical information is in the top 3 most important features in almost a third of the experiments performed. Additionally, this feature is found to be more valuable than the widely used named entity recognition.