bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Next-Generation Sequencing on Diagnosis of Bloodstream Infection Caused by Mycoplasma hominis in a Patient With ANCA-Associated Vasculitis
Authors: Chen Yili , Chen Hengxin , Huang Hao , Zhong Yinyin , Lin Xiaoshu , Chen Peisong , Liao Kang , Liu Min , Hong Mengzhi , Wang Ruizhi 
Published: September 2022

Abstract
Background: Mycoplasma hominis is one of the main opportunistic pathogenic Mycoplasma in human which has a major impact on patients with bloodstream infections. Because it is difficult to detect or isolate, the rapid and accurate diagnosis is essential and still challenging for patients with bloodstream infection.
Case presentation: In this case, we reported the application of next -generation sequencing on diagnosis of bloodstream infection caused by Mycoplasma hominis in a patient with ANCA-associated vasculitis. After 9 days of combined treatment with levofloxacin, polymyxin B, and meropenem, the patient’s infected situation was gradually controlled and he was discharged without further complications. During the three-month outpatient follow-up, no recurrence of symptoms or signs was reported.
Conclusions: This successful application of the next generation sequencing assisted the rapid diagnosis of Mycoplasma hominis bloodstream infection, provided a new perspective in the clinical approach and highlighted the potential of this technique in rapid etiological diagnosis.
The effects of intranasal implantation of mesenchymal stem cells on nitric monoxide levels in the hippocampus, control of cognitive functions and motor activity in a model of cerebral ischemia in rats
Authors: Yafarova Guzel , Tоkalchik Yulia , Filipovich Tatiana , Andrianov Viacheslav , Bazan Lyeo , Bogodvid Tatiana , Chihab Abdulla , Zamaro Aleksandra , Kulchitsky Vladimir , Gainutdinov Khalil 
Published: September 2022

Abstract
Hypoxia occurs in situations of disbalancebetween metabolic needs and the supply of oxygen to organs and tissues of the body. In this regard, tissue hypoxia and ischemia are essential components of the pathogenesis of many diseases. One of the promising areas of research into the mechanisms of ischemia is attempting to weaken the negative effect of hypoxia and ischemia in the brain by using a variety of techniques that activate neuroprotective mechanisms. Here, we aimed to assess the dynamics of restoration of motor activity control in an experimental model of ischemic stroke in rats (cerebral ischemia – CI) after intranasal perineural implantation of mesenchymal stem cells into the receptive field of the olfactory nerve. It was found that the perineural administration of MSCs to rats in the acute period of cerebral ischemia was accompanied by clear signs of recovery of cognitive and motor functions within 1 and 3 days after the operation. On the seventh day after ischemia modeling, rats with the introduction of MSCs had no distinctive features in the control of motor activity compared to the period before the operation in the same rats. In the hippocampus of rats after modeling ischemia, a significant decrease in the content of NO by about 50% relative to the initial level is observed after one day. In the hippocampus of rats in which ischemia was modelled with simultaneous intranasal administration of MSC, a significant decrease in NO content by 39% relative to the initial level was also observed after one day. The content of NO increases slightly, but the difference in the level of NO relative to ischemic rats was not significant. The copper content in the hippocampus in the rats of these two groups did not change. There was a tendency to increase the efficiency of the antioxidant system 1 day after ischemia in both studied groups, and this effect was more pronounced with intranasal administration of MSC.
An Agent-based Approach to Continuously Detect and Update Road Network Changes Using GPS Trajectories
Authors: Mohammadi Reza , Taleai Mohammad , Farnaghi Mahdi 
Published: September 2022

Abstract
Up-to-date road maps are critical in both intelligent transportation and urban management systems. The long cycle of road map generation and updating, and on the other hand, the high rate of expanding public transport, causes the road maps generally be behind the latest actual conditions. The previous studies in road map updating are generally static, applied to the whole study area instead of considering merely the changed road segments, and cannot dynamically respond to the rapid changes in the road networks. This study proposes an agent-based system that dynamically explores newly collected trajectory data and updates road geometry and road type labels. A heuristic change detection algorithm is exploited to detect road network changes (newly added or blocked roads). A road geometry extraction algorithm was developed to digitize newly added roads from the trajectories automatically. Road type labels were predicted using the Random Forest algorithm based on Spatiotemporal properties (speed, acceleration, and standard deviation) of the trajectory data. Finally, the road network map was updated using the newly generated geometry and road type label. GeoLife dataset and OpenStreetMap road network were used to evaluate the proposed approach. Besides, a sensitivity analysis was performed to test the robustness of the results of the road extraction algorithm under various parameter settings. The performances of the road-type prediction algorithms were evaluated using Accuracy, Kappa, Recall, and Precision. Experiments demonstrated the feasibility of the proposed approach for detecting additive road changes and dynamically updating road geometries and types without human intervention.
Coixendide Efficacy in Combination with Temozolomide in Glioblastoma and Transcriptome Analysis of the Mechnism
Authors: Zhao Zhenran , Zhang Lei , Zhang Xiaohan , Yue Yong , Liu Shenchen , Li Yanan , Ban Xiang , Jin Peng , Zhao Cuizhu 
Published: September 2022

Abstract
Purpose: The purpose of this study was to explore the role of Coixendide (Coix) combine with Temozolomide (TMZ) in the treatment of Glioblastoma(GBM) and explore its possible mechanism.
Methods: CCK-8 was used to determine the inhibitory rate of Coix group, TMZ group and drug combination group on GBM cells, and the combination index (CI) was calculated to determine whether they had synergistic effect. Then RNA was extracted from each group, transcriptome sequencing was performed, and differentially expressed genes (DEGs) were identified. The possible mechanism was analyzed by GO enrichment analysis and KEGG enrichment analysis.
Results:The CI of Coix and TMZ indicating a synergistic effect when TMZ concentration is 0.1mg/ml and Coix concentration is 2mg/ml. Transcriptome sequencing analysis showed that interferon (IFN) related genes were down-regulated by Coix and up-regulated by TMZ and combined drugs, however, the up-regulation induced by combined drugs was less than that of TMZ. Besides IFN related genes, cholesterol metabolism pathway were also been regulated.
Conclusion: Coix and TMZ have synergistic effects in the treatment of GBM at certain doses. RNA-Seq results suggested that the abnormal on genetic materials caused by DNA damage induced by TMZ treatment can be sensed by IFN related genes and activates antiviral IFN signaling, causing the activation of repairing mechanism and drug resistance. Coix inhibits IFN related genes, thereby inhibits drug resistance of TMZ. In addition, the activation of ferroptosis and the regulation of DEGs in cholesterol metabolism pathway were also contributed to the synergistic effects of Coix and TMZ.
Early 7,8-Dihydroxyflavone administration ameliorates synaptic and behavioral deficits in the young FXS animal model by acting on BDNF-TrkB pathway
Authors: Chen Yu-shan , Zhang Si-ming , Zhu Qiong , Yue Chao-xiong , Xiang Peng , Li Jin-quan , Wei Zhen , Zeng Yan 
Published: September 2022

Abstract
Fragile X syndrome (FXS) is the leading inherited form of intellectual disability and the most common known cause of autism spectrum disorders. FXS patients exhibit severe syndromic features and behavioral alterations, including anxiety, hyperactivity, impulsivity, and aggression, in addition to cognitive impairment and seizures. At present, there are no effective treatments or cures for FXS. Previously, we have found the divergence of BDNF-TrkB signaling trajectories is associated with spine defects in early postnatal developmental stages of Fmr1 KO mice. Here, young fragile X mice were intraperitoneal injection of 7,8-Dihydroxyflavone (7,8-DHF), which is a high affinity tropomyosin receptor kinase B (TrkB) agonist. 7,8-DHF ameliorated morphological abnormities in dendritic spine and synaptic structure, and rescued synaptic and hippocampus-dependent cognitive dysfunction in young FXS mice. These observed improvement of 7,8-DHF involved decreased protein levels of BDNF, p-TrkBY816, p-PLCγ, and p-CaMKII in the hippocampus. In addition, 7,8-DHF intervention in primary hippocampal neurons increased p-TrkBY816 through activating the PLCγ1-CaMKII signaling pathway leading to improvement of neuronal morphology. This study is the first to account for early life synaptic impairments, neuronal morphological and cognitive delays in FXS in response to the abnormal BDNF-TrkB pathway. Present studies provide novel evidences about the effective early intervention in FXS mice at developmental stages as a strategy to produce powerful impacts on neural development, synaptic plasticity and behaviors.
Economic Freedom and Debt: An Empirical Investigation of the Institutional Determinants of Public Debt.
Authors: Magnini Gino , Vojnovic Jovan 
Published: September 2022

Abstract
This paper investigates the role of the institutional framework in the accumulation of
central government debt. We employ the Economic Freedom Index (EFI) as a proxy for
institutional framework and study the causal link between institutions that promote economic
freedom and the creation of public debt. The results presented in this paper are the
evidence of a direct causal link between institutions and debt ratios. They suggest that
not only the institutional and structural framework of economies matter in public debt
accumulation, but also show that countries with higher institutional scores are less likely
to accumulate high levels of public debt. In particular, a 10-point increase in the economic
freedom score, on average, reduces a country’s debt stock by 7 percentage points. Government
size plays a major role in debt reduction, but it does so in different ways across
corresponding samples. Developing countries can more effectively reduce debt through
a cutback on the tax burden, whereas developed countries are more likely to do so with
policy oriented in reducing government spending. We also find evidence on the importance
of legal institutions in developed countries, especially in the forms of enforcement
of property rights and judicial corruption, in determining levels of government debt.
Molecular characterization of a novel victorivirus isolated from the phytopathogenic fungus Phaeobotryon rhois
Authors: Zhao Ying , Du Hongyan , Liu yuanyuan , Zhong Rongrong , Guan Zhengzhe , Wang Ganlin , Zhang Yuanyuan , Wei Chenxing , Wang Mengjiao , Wan Xinru , Zang Rui , Wen Caiyi , Meng Haoguang 
Published: September 2022

Abstract
Phaeobotryon rhois is an important pathogenic fungus that causes dieback and canker disease of woody hosts. Here, a novel mycovirus, tentatively named Phaeobotryon rhois victorivirus 1 (PrVV1), was identified from P.rhois strain sx8-4. The PrVV1 has a double-stranded RNA (dsRNA) genome of 5,224 nucleotides long and it contains two open reading frames (ORF1 and ORF2), which overlap at a AUGA sequence. ORF1 encodes a polypeptide of 786 amino acids (aa) with a conserved a coat protein (CP) domain, while ORF2, encodes a large polypeptide of 826 aa with a conserved RNA-dependent RNA polymerase (RdRp) domain. Our analysis of the genomic structure, homology searches, and phylogeny indicated that PrVV1 is a novel member of the genus Victorivirus in the family Totiviridae. This is the first study to report the complete genome sequence of a victorivirus that infects P.rhois.
Risk assessment of hazard factors on drowning incidents in Turkey
Authors: Alkan Sabri , Karadurmuş Uğur 
Published: September 2022

Abstract

Drowning, mainly originating from natural factors, is a significant source of hazards worldwide. In this study, the effects and risk assessment of natural and other hazard factors on unintentional drowning cases (UDI) between 2010 and 2020 in Turkey were investigated for the first time. An average of 935 UDIs recorded each year during the study period. 66.1% of UDIs resulted in a fatality, corresponding to a fatality rate of 0.79 per 100,000 population. The highest UDIs rate, with an average of 30.13 per 100,000 population, was recorded in Bartın province, located on the south coast of the Black Sea. The analysis and previous studies show that rip current is a lead cause of natural hazard in drowning incidents on the south coast of the Black Sea. Another significant natural factor in drowning incidents is temporal distribution by months and seasons. The study identified warm seasons as a risk-increasing factor because the number of UDIs was at record levels. According to the number of incidents, almost 80% of victims suffer from drowning during swimming and recreating activities in the surf zone and natural or artificial coastal structures. The study revealed that different natural factors of the incident location, such as meteorological, oceanographic, and surf zone characteristics of beaches, significantly affect the cause of drowning.
Laboratory-based efficacy evaluation of Bacillus thuringiensis subspecies israelensis and temephos larvicides against larvae of Anopheles stephensi in Ethiopia
Authors: Teshome Abebe , Erko Berhanu , Golassa Lemu , Yohannes Gedeon , Irish Seth R. , Zohdy Sarah , Dugassa Sisay 
Published: September 2022

Abstract
Background: Malaria, transmitted by the bite of infected female Anopheles mosquitoes, remains a global public health problem. The presence of an invasive Anopheles stephensi, capable of transmitting Plasmodium vivax and P. falciparum parasites was first reported in Ethiopia in 2016. The ecology of An. stephensi is different from that of An. arabiensis, the primary Ethiopian malaria vector, and this suggests that alternative control strategies may be necessary. Larviciding may be an effective alternative strategy, but there is limited information on the susceptibility of Ethiopian An. stephensi to common larvicides. This study aimed to evaluate the efficacy of temephos and Bacillus thuringiensis var. israelensis (Bti) larvicides against larvae of invasive An. stephensi.
Methods: The diagnostic doses of two larvicides, temephos (0.25ml/l) and Bti (0.05mg/l) were tested in the laboratory against the immature stages (late third to early fourth stages larvae) of An. stephensi collected from the field and reared in a bio-secure insectary. Larvae were collected from two sites (Haro Adi and Awash Subuh Kilo). For each site, three hundred larvae were tested against each insecticide (as well as an untreated control), in batches of 25. The data from all replicates were pooled and descriptive statistics prepared.
Results: The mortality of larvae exposed to temephos was 100% for both sites. Mortality to Bti was 99.7% at Awash and100% at Haro Adi site.
Conclusions: Larvae of An. stephensiare susceptible to temephos and Btilarvicides suggesting that larviciding with these insecticides as vector control program may be effective against An. stephensi in these localities.
Assessment of the inferior vena cava collapsibility from subcostal and trans-hepatic imaging using both M-mode or artificial intelligence: a prospective study on healthy volunteers.
Authors: Sanfilippo Filippo , Via Luigi La , Dezio Veronica , Santonocito Cristina , Amelio Paolo , Genoese Giulio , Astuto Marinella , Noto Alberto 
Published: September 2022

Abstract
Background
Assessment of the inferior vena cava (IVC) respiratory variation may be clinically useful but imaging from subcostal (SC, sagittal) region is not always feasible. It is unclear if coronal trans-hepatic (TH) IVC imaging provides interchangeable results as compared to SC imaging. In this context, the use of artificial intelligence (AI) with software for automated border detection may be clinically helpful but needs validation.
Methods
We conducted a prospective observational study in healthy volunteers assessing the IVC collapsibility (IVCc) in both SC and TH imaging; IVCc measures were taken in M-mode and with the AI method. We calculated the mean bias and the limits of agreement (LoA), as well as the intra-class correlation (ICC) coefficient with 95% confidence intervals.
Results
Sixty volunteers were included; IVC was not visualized in five of them (n = 2, both for SC and TH windows, 3.3%; n = 3 IVC not seen in TH approach, 5%). Compared with standard M-mode, the AI assessment showed good accuracy both for SC (IVCc: bias − 0.7%, LoA [-24.9;23.6]) and TH approach (IVCc: bias 3.7%, LoA [-14.9;22.3]); moreover, the ICC coefficients showed moderate reliability: 0.57 [0.36;0.73] in SC, and 0.72 [0.55;0.83] in TH. Comparing results obtained at different anatomical sites (SC vs TH), the results produced by M-mode assessment were not interchangeable (IVCc: bias 13.9%, LoA [-18.1;45.8]). When this evaluation was performed with the AI software, such difference became smaller: IVCc bias 7.7%, LoA [-19.2;34.6]. The correlation between SC and TH assessments was poor for M-mode (ICC = 0.08 [-0.18;0.34]), whilst moderate for AI (ICC = 0.69 [0.52;0.81]).
Conclusions
The AI software shows good accuracy when compared with the traditional M-mode IVCc assessment conducted at the same anatomical site (both at SC or TH level). There are clinically relevant differences when comparing results obtained at different sites (SC vs TH). Although AI may reduce differences between sagittal and coronal IVCc measurements, the results from different sites do not seem clinically interchangeable.
Long-term benefit of immunotherapy in a patient with mismatch repair deficient/microsatellite instability-high/high tumor mutational burden lung cancer: case report and literature review
Authors: Li Na , Wan Zixuan , Lu Dongyan , Chen Ruilian , Ye Xiaowei 
Published: September 2022

Abstract
Genetic mutations that cause defective mismatch repair, may result in microsatellite instability, which is common in colorectal carcinoma and gastric cancers as well as Lynch syndrome. Mismatch repair deficiency/microsatellite instability-high(dMMR/MSI-H) predicts the tumor response to immune checkpoint inhibitors. However, few studies have evaluated the effects of immune checkpoint inhibitors on dMMR/MSI-H non-small cell lung cancer (NSCLC). We report here a squamous lung cancer patient with dMMR/MSI-H and high tumor mutational burden(TMB-H) who obtained the long-term benefit from immunotherapy. We consider that NSCLC patient with dMMR/MSI-H can benefit from immune checkpoint inhibitors.
Gain Flatness of Amplifier in C-Band using EDFA and RFA for WDM System
Authors: Hassan Abdallah Mohamed , El-Mashade Mohamed B. 
Published: September 2022

Abstract
The gain flatness of amplifier is very important parameter in optical network systems when wavelength division multiplexing (WDM) device used in the transmission of many channels in the system. This paper seeking toward minimum gain flatness of the amplifier. In this paper several methods are used to obtain the optimum value of gain variation and get the maximum gain uniformity between sixteen channels in C band. We optimize the length and pump power of EDFA, and used a lot of stages of EDFA and used hybrid amplifier with EDFA and Raman fiber amplifier (RFA) and optimize the number of pump wavelengths of the RFA. The gains are flattened within 37.65 ± 0.0445 dB from 1546nm to 1552nm with noise figure (NF) within 3.56 dB, Q-factor of 9.97 and BER of 9.3×10− 24 at hybrid 980-EDFA-RFA. A comparison between the several configurations is made.
Classical conditioning of paired-pulse TMS induced intracortical facilitation – a proof of concept
Authors: Ewers Stefan P. , Dreier Timo M. , Al-Bas Siham , Schwenkreis Peter , Pleger Burkhard 
Published: September 2022

Abstract
Background
In this proof-of-concept study, we questioned whether the transient influence of TMS on cortical excitability can be applied to classical conditioning.
Objective
More specifically, we investigated whether the faciliatory influence of paired-pulse TMS on the excitability of the human motor cortex can be transferred to a simultaneously presented auditory stimulus through conditioning.
Methods
During the conditioning phase, 75 healthy young participants received 170 faciliatory paired TMS pulses (1st pulse at 95% resting motor threshold (MT), 2nd at 130% MT, interstimulus interval 12ms), always presented simultaneously with one out of two acoustic stimuli. In the test phase, 20 min later, we pseudorandomly applied 100 single TMS pulses (at 130% MT), 50 paired with the conditioned tone − 50 paired with a control tone.
Results
Comparing the amplitude of the motor evoked potential (MEP) paired with the conditioned tone to the control tone, we found significantly enhanced MEP amplitudes, suggesting successful conditioning (p = 0.03, 55% responder rate). Correlation analyses revealed that the MEP amplitude after paired-pulse stimulation in the conditioning phase related to the MEP single-pulse amplitude combined with the conditioned tone in the test phase (r = 0.603, p < 0.001), and the overall effect of conditioning (i.e., amplitude conditioned tone/control tone; r=-0.213, p = 0.068), suggesting that the higher the amplitudes during conditioning, the higher also the amplitudes during the following test phase and the weaker the effect of conditioning. We next compared single-pulse amplitudes paired with the conditioned tone vs. control tone only in those participants with a paired-pulse amplitude < 2mV in the conditioning phase. Responder rate in this group increased from 55–61% (n = 38, p = 0.01), and even further to 79% (n = 14, p = 0.005) if we considered only those participants with a median paired-pulse amplitude < 1mV, suggesting increasingly stronger conditioning effects for smaller MEP amplitudes after paired-pulse stimulation during conditioning.
Conclusion
Present findings extend the scope of classical conditioning to paired-pulse TMS induced intracortical facilitation.
Relationship Between Social Withdrawal (Hikikomori), Personality, and Coping in an Adult Population
Authors: Bonnaire Céline , Roignot Zoé 
Published: September 2022

Abstract

The aim of this study was to investigate the relationships between personality dimensions, coping strategies, and Hikikomori while controlling for the presence of depression and anxiety. Two groups were compared: the control group (n= 101 participants, mean age ± SD = 36.2 ± 12.8 years) was recruited from several general Facebook groups, and the Hikikomori group (n = 28, mean age ± SD = 30.1 ± 9.1 years) from a private group dedicated to Hikikomori. Participants of both groups completed the Big Five Inventory, the Brief Coping Orientation to Problems Experienced, and the Hospital Anxiety and Depression Scale. Univariate regression analysis revealed that depression, anxiety, neuroticism (and the anxiety facet), self-blame, and behavioral disengagement were significant predictors of Hikikomori. These findings contribute to a better understanding of the psychological functioning of Hikikomori as well as to treatment elaboration.