bluuebunny/crossref_metadata_2025_split huggingface dataset:
The prognostic value of radiomic features from pre- and post-treatment 18F-FDG PET imaging in patients with nasopharyngeal carcinoma
Authors: Kim Soo Jeong , Choi Joon Young , Ahn Yong Chan , Ahn Myung-Ju , Moon Seung Hwan 
Published: November 2022

Abstract
Introduction

Positron emission tomography/computed tomography (PET/CT) with 18F-fluorodeoxyglucose (FDG) is widely used for management of nasopharyngeal carcinoma (NPC). Combining the radiomic features of pre- and post-treatment FDG PET images may im-prove tumor characterization and prognostic predication. We investigated prognostic value of radiomic features from pre- and post-radiotherapy FDG PET images in patients with NPC.

Methods

Quantitative radiomic features of primary tumors were extracted from the FDG PET images of 145 NPC patients and the delta values were also calculated. The study population was divided randomly into two groups, the training and test sets (7:3). A random survival forest (RSF) model was adopted to perform analyses of progression-free survival (PFS) and overall survival (OS).

Results

There were 37 (25.5%) cases of recurrence and 16 (11.0%) cases of death during a median follow-up period of 54.5 months. Both RSF models with clinical variables and radiomic PET features for PFS and OS showed comparable predictive performance to RSF models with clinical variables and conventional PET parameters.

Conclusions

Tumoral radiomic features of pre- and post-treatment FDG PET and the corresponding delta values may predict PFS and OS in patients with NPC.
β1,3-galactosyltransferase on chromosome 6 is essential for the formation of Lewis a structure on N- glycan in Oryza sativa
Authors: Jung Jae-Wan , Kim Seong‑Ryong 
Published: November 2022

Abstract
β1,3-galactose is the component of outer-chain elongation of complex N-glycans that, together with α1,4-fucose, forms Lewis a structures in plants. Previous studies have revealed that N-glycan maturation is mediated by sequential attachment of β1,3-galactose and α1,4-fucose by individual β1,3-galactosyltransferase (GalT) and α1,4-fucosyltransferase (1,4-FucT), respectively. Although GalT from several species has been studied, little information about GalT from rice is available. I therefore characterized three GalT candidate genes on different chromosomes in Oryza sativa. Seeds of rice lines that had T-DNA insertions in regions corresponding to individual putative GalT genes were obtained from a Rice Functional Genomic Express Database and plants grown until maturity. Homozygotes were selected from the next generation by genotyping PCR, and used for callus induction. Callus extracts of two independent T-DNA mutant rice which have T-DNA insertions at the same gene on chromosome 6 but in different exons showed highly reduced band intensity on a western blots using an anti-Lewis a antibody. Cell extracts and cultured media from suspension culture of the one of these mutant rice were further analysed by N-glycan profiling using matrix-associated laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF). Identified N-glycan species containing β1,3-galactose from both cell extracts and cultured media of knock-out mutant were less than 0.5% of total N-glycans while that of WT cells were 9.8% and 49.1%, respectively. This suggests that GalT located on rice chromosome 6 plays a major role in N-glycan galactosylation, and mutations within it lead to blockage of Lewis a epitope formation.
Preoperative pericardial hematoma in patients with acute type A aortic dissection (AAAD): Do we need an adjusted treatment?
Authors: Kaufeld Tim , Beckmann Erik , Rudolph Linda , Krüger Heike , Natanov Ruslan , Arar Morsi , Korte Wilhelm , Schilling Tobias , Haverich Axel , Martens Andreas , Shrestha Malakh 
Published: November 2022

Abstract
Objective
An acute type A aortic dissection (AAAD) is a critical emergency and remains one of the most challenging diseases in cardiothoracic surgery. The existence of a pericardial hematoma caused by an aortic rupture can dramatically reduce the chances of survival [1–4]. We assessed the surgical outcome of a high-risk group of patients with AAAD and a pericardial hematoma.
Methods
In this study we included 430 Patients (67% male; median age: 64 years) who received surgical treatment between January 2000 and January 2018 at our facility for acute aortic dissection DeBakey type I. We divided the cohort in two groups: Group A consisted of high-risk patients with a pericardial hematoma (n = 162) and Group B of patients without pericardial hematoma (n = 268).
Results
Patients with a preoperative pericardial hematoma had a significantly higher requirement for preoperative mechanical resuscitation (A: 21%; B: 1.5%; P: <0.001) and were relevantly more frequently admitted to the operation theater with an intubated status (A: 19.8%; B: 8.6%; P: <0.001). The incidence of visceral malperfusion differed significantly between both groups (A. 11.7%, B. 6:0%; P. 0.034). Limited aortic arch repair (proximal aortic arch replacement) was preferred in the high-risk group (A: 51.9%; B: 40.3%; P: 0.020). However, survival time was generally reduced in these patients (A: 7.5 y; B: 9.9 y)
Conclusion
AAAD patients with preoperative pericardial hematoma present themselves in potentially lethal conditions, with a significantly higher rate of visceral malperfusion. Despite the existence of this risk factor, a limited arch repair was favored. According to our data, based on the surgeon’s decision, a limited aortic repair is preferred in these compromised cases. Our study reveals that extended aortic surgery might contribute substantially to a favorable outcome.
Larvicidal activity assessment for anticholinesterase insecticides against laboratory and field strains of Egyptian cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae)
Authors: Ayoub Haytham A. , Khairy Mohamed 
Published: November 2022

Abstract
Anticholinesterase insecticides are widely applied in crop protection programs for the chemical control of Egyptian cotton leafworm (Spodoptera littoralis).Therefore, the continuous evaluation for the insecticidal activity against sensitive (laboratory) and resistance (field) strains is very important to reconsider their applicability. Herein, the larvicidal activity of carbamate esters (methomyl) and organophosphorus (chlorpyrifos) was evaluated via feeding bioassay technique under laboratory conditions against second instar larvae of the Egyptian cotton leafworm (spodoptera littoralis). The results reviled that the larvicidal activity for the laboratory strain was higher than the field strain for both methomyl (LC50 = 413.0 ppm, LC50 = 581.49 ppm) and chlorpyrifos (LC50 = 219.73 ppm, LC50 = 275.94 ppm) insecticides. Moreover, the resistance of chlorpyrifos (RR = 1:1.26) was lower than that for methomyl (RR = 1:1.41) in laboratory and field strains, respectively.
[8] and [10]-Gingerol reduces urothelial damage in ifosfamide-induced hemorrhagic cystitis via JAK/STAT/FOXO signaling pathway via IL-10
Authors: Ferreira Francisco C. S. , Clementino Marco , Rodrigues Francisco A. P. , Veras Herlice N. , Martins Dainesy S. , Queiroga Marcus L. , Lima Mikael A. , Silva Dayara O. , Freitas Thiago M. , Ribeiro Samilly A. , Mota Mario R. L. , Silva James A. , Lima Aldo A. M. , Havt Alexandre 
Published: November 2022

Abstract
Acrolein is the main toxic metabolite of Ifosfamide (IFO) that causes urothelial damage by oxidative stress and inflammation. Here we investigate the molecular mechanism of action of gingerols, Zingiber officinale bioactive molecules, as an alternative treatment for ifosfamide-induced hemorrhagic cystitis. Female Swiss mice were randomly divided into 5 groups: control; IFO; IFO + Mesna; and IFO + [8]- or [10]-gingerol. Mesna (80 mg/kg, i.p.) was given 5 minutes before, 4 and 8 hours after IFO (400mg/kg, i.p.). Gingerols (25 mg/Kg, p.o.) were given 1 hour before and 4 and 8 hours after IFO. Animals were euthanized 12 hours after IFO injection. Bladders were submitted to macroscopic and histological evaluation. Oxidative stress and inflammation were assessed by malondialdehyde (MDA) or myeloperoxidase assays, respectively. mRNA gene expression was performed to evaluate Mesna and gingerols mechanisms of action. Mesna was able to protect bladder tissue by activating NF-κB and NrF2 pathways. However, we demonstrated that gingerols acted as an antioxidant and anti-inflammatory agent stimulating the production of IL-10, which intracellularly activated JAK/STAT/FOXO signaling pathway.
Systematic analysis of paralogous regions in 41,755 exomes uncovers clinically relevant variation
Authors: Steyaert Wouter , Haer-Wigman Lonneke , Pfundt Rolph , Hellebrekers Debby , Steehouwer Marloes , Hampstead Juliet , de Boer Elke , Stegmann Alexander , Yntema Helger , Kamsteeg Erik-Jan , Brunner Han , Hoischen Alexander , Gilissen Christian 
Published: November 2022

Abstract
We devised a new method (Chameleolyser) that accurately identifies single nucleotide variants (SNVs), copy number variants and ectopic gene conversion events in duplicated genomic regions using whole-exome sequencing (WES) data. Application to a cohort of 41,755 WES samples yielded 20,432 rare homozygous deletions and 2,529,791 rare SNVs, of which we can show that 338,084 are due to gene conversion events. None of the SNVs are detectable using regular analysis techniques. Validation by high-fidelity long-read sequencing in 20 samples confirmed >88% of called variants. Focusing on variation in known disease genes led to a direct molecular diagnosis in 25 previously undiagnosed patients. Our method can readably be applied to existing WES data.
Determination of fumigant toxicity of single, binary and tertiary mixtures of three essential oils against Frankliniella occidentalis Pergande (Thysanoptera: Thripidae)
Authors: KIRIŞIK Musa 
Published: November 2022

Abstract
In this study, three essential oils extracted from sage (Salvia officinalis L.), thyme (Thymus vulgaris L.) and daphne (Laurus nobilis L.) were used against the adult Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) pest, one by one and more Then, fumigant toxicity of binary (1:1) and tertiary (1:1:1) mixtures was investigated under laboratory conditions. The results showed that all essential oils tested, both alone and in all binary and tertiary mixtures, had fumigant toxicity on adult F. occidentalis. Mortality rates of 3–5 day old adults of F. occidentalis increased with increasing concentration from 20 to 100 µL/L. In the entire study, binary and tertiary mixtures of the oils were more successful than the individual application. At a dose of 10 µL/L, all binary and tertiary mixtures of the oils caused 100% mortality at all exposure time periods. However, in the application where sage oil was fumigated for 24 hours and applied alone, the mortality rate remained below 100%. The highest synergistic effect was found in triple essential oil mixtures (sage + daphne + thyme) with 1.0 µL/L air LC50 value in all application doses, counted after 48 hours. Our overall results show that binary and tertiary mixture of all tested essential oils are promising candidates for protection against western flower thrips.
Magnesium supplementation during pregnancy may strengthen the placental anti-inflammatory response
Authors: Önal Mesut , Kocaman Adem , Katirci Yunus , Ayas Bulent , Ozdemir Ayse Zehra , Kocak Idris 
Published: November 2022

Abstract
The formation, development, and survival of the placenta are critical for the successful conclusion of pregnancy. Placental oxidative stress, inflammation, and other possible threats may adversely affect the development of the placenta. Magnesium, known to be a potent anti-inflammatory, may have positive effects on placental development. This study aimed to investigate the potential anti-apoptotic and anti-inflammatory properties of magnesium. 12 normal and 12 magnesium supplementation-used patients were included in the study. After delivery, placentas were collected, and Bcl-2, Ki-67, and Collagen IV expression levels were analyzed immunohistochemically. While there was a statistical difference in Bcl-2 and Collagen IV expression levels, no difference was found in Ki-67 expression levels. Anti-apoptotic and anti-inflammatory properties were seen in the magnesium supplementation-used patients. Using magnesium during pregnancy may be beneficial in terms of placental development.
Long-term outcome of CO 2 insufflation in thoracoscopic esophagectomy after neoadjuvant chemotherapy for esophageal squamous cell carcinoma: A retrospective cohort study Short title: Outcome of CO 2 insufflation in esophagectomy
Authors: Otsuka Koji , Goto Satoru , Ariyoshi Tomotake , Yamashita Takeshi , Saito Akira , Kohmoto Masahiro , Kato Rei , Motegi Kentaro , Yajima Nobuyuki , Murakami Masahiko 
Published: November 2022

Abstract
Background
Thoracoscopic esophagectomy, with CO2 insufflation as a prerequisite, is increasingly performed for esophageal cancer. However, there is limited evidence on the influence of CO2 insufflation on the long-term postoperative survival. We aimed to estimate long-term outcomes with or without CO2 insufflation during thoracoscopic esophagectomy.
Methods
We enrolled 182 patients who underwent thoracoscopic esophagectomy for esophageal cancer between January 2003 and October 2013 and categorized them into two groups: with and without CO2 insufflation. The primary endpoint was the 5-year overall survival (5y-OS). Secondary endpoints included other long-term (i.e., 5-year relapse-free survival [5y-RFS] and 5-year cancer-specific survival [5y-CSS]) and short-term (i.e., surgical and non-surgical complications and 30-day reoperation) outcomes.
Results
Follow-up until death or the 5-year postoperative period was 98.9% complete (median follow-up duration: 6 years, in survivors). After adjusting for age and sex, the analyses showed no significant differences in 5y-OS (hazard ratio [HR] 0.84, 95% confidence interval [CI] 0.50–1.40), 5y-RFS (0.79, 0.49–1.31), and 5y-CSS (0.74, 0.43–1.27). For short-term outcomes, significant intergroup differences in the thoracic operative time (p = 0.02), thoracic blood loss (p < 0.001), postoperative length of stay (p < 0.001), and atelectasis (p = 0.004) were observed, and the results of sensitivity analysis were similar to the main results.
Conclusions
CO2 insufflation in thoracoscopic surgery for esophageal cancer seemingly does not change the long-term prognosis and is possibly safe in other thoracic procedures.
Light Weight Plasters Containing Vermiculite and FGD Gypsum Wastes for Sustainable and Energy Efficient Building Construction Material
Authors: Maiti Soumitra , Jain Neeraj , Malik Jaideep , - Aakriti 
Published: November 2022

Abstract
Development of light weight plasters for mortar rendering utilizing FGD gypsum have been reported here. Lightweight plasters prepared using FGD gypsum and exfoliated vermiculite were characterized and studied in detail for interior wall applications. Different gypsum vermiculite plasters (GVP) with variable amounts of vermiculite were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermal gravimetric analysis (TG-DTG). The physicochemical and mechanical properties of all the samples are determined and considered to be efficient for interior applications. An optimum mix composition was selected based on its compressive strength, water absorption and porosity. Water absorption and porosity studies restrict the usage of GVP only to interior wall purposes. The acoustic performance of the materials revealed good sound absorption (α = 0.65). Plasters exhibit satisfactory durability under severe conditions of winter and summer weather. GVP shows excellent fire resistance under BS 476–1997 fire resistance classification with thermal conductivities (< 0.161 W/mK) much lower than standard building materials, which makes them fit for energy efficient insulation materials. These studies depict the efficient utilization of thermal power plant waste, FGD gypsum in interior wall insulation for mortar rendering and can be further extended to exterior construction applications by reducing water absorption.
Atmospheric Correction of FG5 Absolute Gravimetry Data using Measured Air Pressure
Authors: Zhai Dulin , Liu Ziwei , Bai Lei , Zhou Hao , Gou Jianing 
Published: November 2022

Abstract
Changes in atmospheric pressure affect atmospheric density, which is a key factor affecting high-precision gravity measurement. Currently, atmospheric correction of absolute gravity measurements uses the empirical admittance value recommended by the International Association of Geodesy (− 0.3 µGal/mbar); however, the actual admittance value changes with atmospheric mass and time. In this study, we determine the effect of using measured admittance values for absolute gravity correction. First, high-precision relative gravimeters (OSG-057, CG5) are used for continuous gravity measurement. Then, air pressure measured by the FG5 absolute gravimeter is used to obtain the atmospheric admittance using the iterative least squares method, which is compared with the theoretical atmospheric admittance. Taking FG5-257 as an example, we use the measured admittance for atmospheric correction of absolute gravity at four different elevations (Lhasa, Nagqu, Gar, and Suining, China). The results are as follows. 1) FG5-257 can accurately calibrate the Lhasa OSG-057 superconducting gravimeter with precision of 0.01% and a scale factor of − 77.001 ± 0.007µGal/V. 2) According to co-location measurements in Lhasa, CG5 and OSG measured admittance values exhibit comparable precision (0.332 ± 0.0029 µGal/mbar and − 0.332 ± 0.0004 µGal/mbar, respectively), indicating that the time-variable gravity field can be obtained by CG5 without co-location with superconductive gravimeters. 3) After correction using the measured admittance, changes in inter-group dispersion and measurement precision are approximately 0.01 µGal; however, the effect on the measurement results does not exceed 1 µGal, which is equivalent to the measurement precision of FG5. Therefore, measured admittance values are only recommended for atmospheric correction of high-precision absolute gravity measurements.
Difference in Expression Profile of Compact and Expanded-Type Equine Cumulus-Oocyte-Complexes
Authors: Shen Yingchao , Ulaangerel Tseweendolmaa , Davshilt Toli , Ren Hong , Wang Xisheng , Yi Minna , Tian Shuyue , Dugarjaviin Manglai , Bou gerelchimeg 
Published: November 2022

Abstract
Background: Typically, 60% of oocytes recovered from equine follicles are with expanded cumuli, 30% are with compact cumuli, and 10% are degenerated. Studies have proved that equine oocytes with expanded cumuli (Ex COCs) and oocytes with compact cumuli (Cp COCs) are of different developmental competence and therefore, need different maturation time in vitro. To better understand the gap in developmental competence, we compared the expression of some growth factors and hormone-related genes in Ex COCs and Cp COCs.
Methods: We tested the expression of some hormones and growth factors in Ex- and Cp-COCs by qPCR and immunofluorescence, and the influence of the growth factors and hormones on equine IVM.
Results: As a result, we found that the expression patterns of FSHR, LHR,IGF1R, IGF2R, ESR1, ESR2, BMPR1, BMPR2, and ALK5 are different in two types of COCs and among BMP15, GDF9, Estrogen, and IGF2, the addition of Estrogen and IGF2 are beneficial to equine IVM.
Conclusions: This study indicates that the differential expression pattern of growth factors and hormone-related genes might be responsible for the difference in developmental capacity of two types of COCs. This work provides clues for improving the equine IVM system.
Comprehensive analysis of the relevance of the CELF gene family to prognosis and immune infiltration in brain glioma
Authors: tan tanlijie , Mo Liyi Mo , Ren Longxin , Yi Lan , Yang Nanyang , Li Guoqing , Meng Lijuan 
Published: November 2022

Abstract
Background
The CELF gene family is a set of highly conserved genes that encode a family of RNA-binding proteins in human tissues and participate in posttranscriptional modifications. The CELF gene family has been reported to be associated with a variety of cancers, including lung cancer, liver cancer and colorectal cancer. However, the expression, function and clinical relevance of CELF family members in glioma remain uncertain because of the lack of relevant studies.
Materials & methods
Bioinformatics analysis was used to determine the prognostic value and immune infiltration associations of CELF gene family members in glioma. Transcription and survival data related to CELF gene family members in glioma patients were obtained from the Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA) and cBioPortal databases.
Results
The results revealed that compared to that in normal tissues, the expression of CELF2-6 was notably lower but that of CELF1 was higher in glioma tissues. Survival analysis using the GEPIA database revealed that high transcript levels of CELF1 were associated with unfavourable OS in all patients with LGG. Conversely, high CELF3/4/5 levels predicted favourable OS in these patients.
Conclusion
This study implied that CELF1/3/4/5 are new prognostic markers and potential therapeutic targets for LGG.
Prevalence, associated risk factors, and antimicrobial susceptibility patterns of Escherichia coli and Escherichia coli O157 isolated from cow milk and milk products and hygienic practices in selected towns of the Oromia region, Ethiopia
Authors: Sarba Edilu Jorga , Wirtu Wakuma , Gebremedhin Endrias Zewdu , Borana Bizunesh Mideksa , Marami Lencho Megersa 
Published: November 2022

Abstract

Background
Escherichia coli is a major foodborne illness pathogen worldwide. This study was conducted to assess the prevalence, risk factors, and antimicrobial susceptibility of E. coli and E. coli O157 in milk and milk products and hygienic practices in West Shewa, Oromia, Ethiopia.
Methods
Five hundred fifty-six milk samples comprising 421 udder milk, 57 bulk tank milk, and 78 milk products were investigated. Moreover, 145 questionnaires were administered to assess hygienic practices. A standard microbiological procedure was used to detect E. coli and E. coli O157:H7. The Kirby Bauer disc diffusion method was used to test the antimicrobial susceptibility of the isolates. Petri film plates were used to enumerate the coliform in raw bulk tank milk. Farm and animal-level E coli prevalence were estimated and association with risk factors was assessed.
Results
E. coli was detected in 33.8% (95% confidence interval (CI): 29.9–37.9%) of the samples, of which only one isolate (0.2%) was E.coli O157. E. coli contamination was higher in bulk tank samples (47.4%; 95% CI: 34.0– 61.0%) than in udder milk (34.7%; CI: 30.1–39.4%) and in cottage cheese (27.0%; 95% CI: 14.6–43.9%) than in yoghurt 10.5% (95% CI: 2.9–24.8%). For the animal-level E. coli contamination, only the study area was identified as a risk factor. Risk factors such as types of milk containers, udder washing practices, hygiene, and management systems were associated with both farm-level and milk products E.coli contamination. Most (59.0%) of the farmers practice udder washing only before milking and the remaining did not practice udder washing at all. The mean coliform count in raw bulk tank milk was higher than the international standard (4.09 log10 CFU/ml). All of the 42 E.coli isolates tested were multidrug resistant.
Conclusions
The occurrence of E. coli is high, while that of E. coli O157 is too low. The milk-handling practices are poor in the study areas. The high prevalence of E. coli, as well as the high coliform count, and higher multi-drug resistance may pose risk to public health and food safety. Therefore, proper hygienic practices throughout the milk chain as well as rational drug use are advised.