bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba
Authors: Navarrete Maylin González , Hodžić Adnan , Corona-González Belkis , Cordeiro Matheus Dias , da Silva Claudia Bezerra , Báez Liani Coronado , Obregón Dasiel , de Aguiar Daniel Moura , da Silva Campos Amanda Noeli , Taques Ísis Indaiara Gonçalves Granjeiro , Wu-Chuang Alejandra , López Eugenio Roque , Piloto-Sardiñas Elianne , Abuin-Denis Lianet , da Fonseca Adivaldo Henrique , Cabezas-Cruz Alejandro 
Published: August 2022

Abstract
Background
Canine monocytic ehrlichiosis (CME) is caused by the tick-borne pathogen Ehrlichia canis, an obligate intracellular Gram-negative bacterium of the family Anaplasmataceae with tropism for canine monocytes and macrophages. The trp36 gene, which encodes for the major immunoreactive protein TRP36 in E. canis, has been successfully used to characterize the genetic diversity of this pathogen in different regions of the world. Based on trp36 sequence analysis, four E. canis genogroups, United States (US), Taiwan (TWN), Brazil (BR) and Costa Rica (CR), have been identified. The aim of this study was to characterize the genetic diversity of E. canis in Cuba based on the trp36 gene.

Methods
Whole blood samples (n = 8) were collected from dogs found to be infested with the tick vector Rhipicephalus sanguineus sensu lato (s.l.) and/or presenting clinical signs and symptoms of CME. Total DNA was extracted from the blood samples and trp36 fragments were amplified by PCR. Nucleotide and protein sequences were compared using alignments and phylogenetic analysis.

Results
Four of the trp36 sequences obtained (n = 8) fall within the phylogenetic cluster grouping the US genogroup E. canis strains. The other E. canis trp36 sequences formed a separate and well-supported clade (94% bootstrap value) that is phylogenetically distant from the other major groups and thus represents a new genogroup, herein designated as the ‘Cuba (CUB) genogroup’. Notably, dogs infected with the CUB genogroup presented frequent hemorrhagic lesions.

Conclusions
The results of this study suggest that genetic diversification of E. canis in Cuba is associated with the emergence of E. canis strains with increased virulence.
Encephalomyelitis associated with coronavirus disease 2019: a case report
Authors: Estiasari Riwanti , Maharani Kartika , Octaviana Fitri , Putri Anyelir Nielya Mutiara , Ramadhan Syifa Laila , Rozaliani Anna , Imran Darma 
Published: August 2022

Abstract
Background
Despite a considerable number of articles regarding neurological manifestations associated with severe acute respiratory syndrome coronavirus 2 infection, reports on transverse myelitis and encephalitis are scarce.

Case presentation
We report a 35-year-old Asian Arab female presenting with longitudinally extensive transverse myelitis within 3 weeks after being diagnosed with mild coronavirus disease 2019 infection. Administration of high-dose methylprednisolone led to significant clinical improvement. However, 2 days after discharge, the patient was readmitted with encephalitis manifestations, consisting of fever and loss of consciousness, along with deterioration in myelitis symptoms. Severe acute respiratory syndrome coronavirus 2 antibody was detected in cerebrospinal fluid, but DNA of severe acute respiratory syndrome coronavirus 2 was not found. Clinical recovery was achieved after the administration of intravenous immunoglobulin.

Conclusion
Longitudinally extensive transverse myelitis can be a neurological manifestation of coronavirus disease 2019 and can be followed by encephalomyelitis episodes. High-dose steroids and intravenous immunoglobulin as an immunomodulator are possible effective treatment options.
Changes in thrombosis-related parameters after AstraZeneca COVID-19 vaccination in a male volunteer: a case report
Authors: Hong Sae-Yong , Jun Sang-Sin , Seo Sang-Wook , Park Jeong-Rae , Im Joung-Il 
Published: August 2022

Abstract
Background
We speculated that subclinical thrombosis may occur frequently through crosstalk between immune/inflammatory reactions and hemostasis after corona virus disease-2019 (COVID-19) vaccination. To test this hypothesis, we measured thrombosis-related parameters after COVID-19 vaccination in a volunteer for 21 days.

Case presentation
The following parameters were measured in a 72-year-old Korean man at 1 day before vaccination and on days 1, 3, 7, 14, and 21 post vaccination (AstraZeneca COVID-19 vaccine: ChAdOx1-S/nCoV-19, CTMAV563): complete blood count, platelet indices, thrombin receptor-activating peptide-induced platelet aggregation, prothrombin time, activated partial thromboplastin time, D-dimer, thrombin–antithrombin III complex (TAT), plasmin-α2 antiplasmin complex (PAP), von Willebrand factor (vWF) antigen and activity, plasminogen activator inhibitor-1 (PAI-1), protein C and protein S antigen and activity, lupus anticoagulant, fibrinogen degradation product, and plasminogen. We found that the TAT had significantly increased from 0.7 ng/mL (baseline) to 21.7 ng/mL (day 1). There was a transient increase in the PAI-1 level from 7.2 ng/mL (baseline) to 10.9 ng/mL (day 3), followed by a decrease in PAP level from 0.9 ng/mL (baseline) to 0.3 μg/mL (day 7), suggesting that plasmin generation is suppressed by PAI-1.

Conclusions
Increased thrombotic factors (such as decreased protein S) and decreased fibrinolytic activity due to increased PAI-1 were potential factors causing thrombogenesis after COVID-19 vaccination. Sequential measurement of platelet indices, TAT, PAP, protein C, protein S, vWF, D-dimer, and PAI-1 following COVID-19 vaccination was informative.
Impact of industry sponsorship on the quality of systematic reviews of vaccines: a cross-sectional analysis of studies published from 2016 to 2019
Authors: Pieper Dawid , Hellbrecht Irma , Zhao Linlu , Baur Clemens , Pick Georgia , Schneider Sarah , Harder Thomas , Young Kelsey , Tricco Andrea C. , Westhaver Ella , Tunis Matthew 
Published: August 2022

Abstract
Background
Systematic reviews (SRs) provide the highest level of evidence and inform evidence-based decision making in health care. Earlier studies found association with industry to be negatively associated with methodological quality of SRs. However, this has not been investigated in SRs on vaccines.

Methods
We performed a systematic literature search using MEDLINE and EMBASE in March 2020. The results were restricted to those published between 2016 and 2019 with no language restrictions. Study characteristics were extracted by one person and checked by an experienced reviewer. The methodological quality of the SRs was assessed with the AMSTAR 2 tool by multiple reviewers after a calibration exercise was performed. A summary score for each SR was calculated. The Mann-Whitney U test and Fisher’s exact test were performed to compare both groups.

Results
Out of 185 SRs that met all inclusion criteria, 27 SRs were industry funded. Those were matched with 30 non-industry funded SRs resulting in a total sample size of 57. The mean AMSTAR 2 summary score across all SRs was 0.49. Overall, the median AMSTAR 2 summary score was higher for the non-industry funded SRs than for the industry-funded SRs (0.62 vs. 0.36; p < .00001). Lower ratings for industry funded SRs were consistent across all but one AMSTAR 2 item, though significantly lower only for three specific items.

Conclusion
The methodological quality of SRs in vaccination is comparable to SRs in other fields, while it is still suboptimal. We are not able to provide a satisfactory explanation why industry funded SRs had a lower methodological quality than non-industry funded SRs over recent years. Industry funding is an important indicator of methodological quality for vaccine SRs and should be carefully considered when appraising SR quality.
Negative emotions and creativity among Chinese college students during the COVID-19 pandemic: the mediating role of psychological resilience and the moderating role of posttraumatic growth
Authors: Zeng Wei , Huang Dongtao , Li Qian , Xu Yanhua , Xu Ziying , Wu Chujin , Chen Zhihao , Yang Yuhao , Shao Jinlian , Wu Xingrou , Zhu Ziqi , Wu Jiamin , Zeng Yuqing 
Published: August 2022

Abstract
Background
The aim of the study was to use a moderated mediation model to understand and examine the relationship between negative emotions and creativity among college students during the COVID-19 pandemic, using psychological resilience as a mediator and posttraumatic growth as a moderator.

Methods
A sample of 881 college students in mainland China completed a self-report questionnaire that included four scales: the Depression-Anxiety-Stress Scale, Psychological Resilience Scale, Runco Ideational Behavior Scale and Posttraumatic Growth Inventory.

Results
Findings indicated that:(1) negative emotions were a strong predictor of creativity; (2) psychological resilience partially mediated the association between negative emotions and creativity; and (3) posttraumatic growth moderated the positive effect of psychological resilience, such that the indirect effect between negative emotions and creativity via psychological resilience was stronger for someone with a low level of resilience.

Conclusion
The findings further clarify the mechanisms that affect the relationship between negative emotions and creativity among college students.
Analysis of gene expression profiles in two spinal cord injury models
Authors: Yuan Haifeng , Zhang Bi , Ma Junchi , Zhang Yufei , Tuo Yifan , Li Xusheng 
Published: August 2022

AbstractObjectivesTo analyze the changes of gene expression at different timepoints after spinal cord injury (SCI) with tenth segment thoracic injury.MethodsTwo SCI models, the complete paraplegia (H) and Allen’s strike (D) methods were applied to induce SCI in rats, and transcriptome sequencing was performed 1, 3, 7, 14, 56, and 70 days after SCI, respectively. Principal component analysis, differentially expressed gene analysis, and hierarchical clustering analysis were applied to analyze the differentially expressed genes (DEGs). Gene Ontology GO enrichment analysis, Kyoto Encyclopedia of Genes and Genomes enrichment analysis, and Gene Set Enrichment Analysis revealed the pathway of gene enrichment.ResultsThere were 1,907, 3,120, 3,728, 978, 2,319, and 3,798 DEGs in the complete paraplegia group and 2,380, 878, 1,543, 6,040, 1,945, and 3,850 DEGs in the Allen’s strike method group and after SCI at 1, 3, 7, 14, 56, and 70 days, respectively. The transcriptome contours of D1, H1, D3, and H14 were clustered with C; the H56, D56, H70, and D70 transcriptome contours were similar and clustered together. H3, D7, and H7 were clustered together, and D14 was clustered separately. The transcriptome differences of the two SCI models were mainly concentrated during the first 2 weeks after SCI. The DEGs after SCI in the complete paraplegia group were more concentrated. Most of the early transcriptional regulation stabilized within 2 weeks after injury.ConclusionsThere were DEGs between the two SCI models. Through the gene changes and pathway enrichment of the entire time period after SCI, the molecular mechanism of SCI repair was revealed in depth, which provided a reference for SCI treatment in the future.
Methods to derive the magnetopause from soft X-ray images by the SMILE mission
Authors: Wang Chi , Sun Tianran 
Published: August 2022

AbstractSolar wind Magnetosphere Ionosphere Link Explorer (SMILE) is a novel self-standing mission dedicated to observing the solar wind–magnetosphere coupling via simultaneous in situ solar wind/magnetosheath plasma and magnetic field measurements, soft X-ray images of the magnetosheath and polar cusps, and UV images of global auroral distributions. While analyzing the observed images after the launch of SMILE, it will be a challenging task to reconstruct the 3-dimensional surface of the magnetopause from 2-dimensional images. Therefore, one of the most important key issues about SMILE is the reconstruction of magnetopause from X-ray images. This paper will review four main approaches have been developed so far, namely, the boundary fitting approach (BFA), the tangent fitting approach (TFA), the tangential direction approach (TDA), and the computed tomography approach (CTA). We will discuss their scope of application and pros and cons, and hopefully inspire future efforts.
Mendel paved the path toward understanding genetic diseases
Authors: Das Sreejon Sundar 
Published: August 2022

Abstract
Background
July 20th, 2022, marks the 200th anniversary of the “Father of Genetics,” Gregor Mendel’s birth. His experiments with pea plants established the fundamental principles of genetic inheritance.

Main text
In this article, a succinct review of literature is hereby done to answer two key questions: (1) How Mendel’s principles of genetic inheritance helped us understand Mendelian disorders? and (2) How the study of Mendelian disorders can help us understand complex diseases?

Conclusion
This literature review concludes that continued effort to understand the genetic basis of Mendelian disorders will improve our understanding and treatment strategies for complex diseases.
Randomized, Double-Blind, Placebo-Controlled, Global Phase III Trial of Talimogene Laherparepvec Combined With Pembrolizumab for Advanced Melanoma
Authors: Chesney Jason A. , Ribas Antoni , Long Georgina V. , Kirkwood John M. , Dummer Reinhard , Puzanov Igor , Hoeller Christoph , Gajewski Thomas F. , Gutzmer Ralf , Rutkowski Piotr , Demidov Lev , Arenberger Petr , Shin Sang Joon , Ferrucci Pier Francesco , Haydon Andrew , Hyngstrom John , van Thienen Johannes V. , Haferkamp Sebastian , Guilera Josep Malvehy , Rapoport Bernardo Leon , VanderWalde Ari , Diede Scott J. , Anderson James R. , Treichel Sheryl , Chan Edward L. , Bhatta Sumita , Gansert Jennifer , Hodi Frank Stephen , Gogas Helen 
Published: August 2022

PURPOSE The combination of talimogene laherparepvec (T-VEC) and pembrolizumab previously demonstrated an acceptable safety profile and an encouraging complete response rate (CRR) in patients with advanced melanoma in a phase Ib study. We report the efficacy and safety from a phase III, randomized, double-blind, multicenter, international study of T-VEC plus pembrolizumab (T-VEC-pembrolizumab) versus placebo plus pembrolizumab (placebo-pembrolizumab) in patients with advanced melanoma. METHODS Patients with stage IIIB-IVM1c unresectable melanoma, naïve to antiprogrammed cell death protein-1, were randomly assigned 1:1 to T-VEC-pembrolizumab or placebo-pembrolizumab. T-VEC was administered at ≤ 4 × 106 plaque-forming unit (PFU) followed by ≤ 4 × 108 PFU 3 weeks later and once every 2 weeks until dose 5 and once every 3 weeks thereafter. Pembrolizumab was administered intravenously 200 mg once every 3 weeks. The dual primary end points were progression-free survival (PFS) per modified RECIST 1.1 by blinded independent central review and overall survival (OS). Secondary end points included objective response rate per mRECIST, CRR, and safety. Here, we report the primary analysis for PFS, the second preplanned interim analysis for OS, and the final analysis. RESULTS Overall, 692 patients were randomly assigned (346 T-VEC-pembrolizumab and 346 placebo-pembrolizumab). T-VEC-pembrolizumab did not significantly improve PFS (hazard ratio, 0.86; 95% CI, 0.71 to 1.04; P = .13) or OS (hazard ratio, 0.96; 95% CI, 0.76 to 1.22; P = .74) compared with placebo-pembrolizumab. The objective response rate was 48.6% for T-VEC-pembrolizumab (CRR 17.9%) and 41.3% for placebo-pembrolizumab (CRR 11.6%); the durable response rate was 42.2% and 34.1% for the arms, respectively. Grade ≥ 3 treatment-related adverse events occurred in 20.7% of patients in the T-VEC-pembrolizumab arm and in 19.5% of patients in the placebo-pembrolizumab arm. CONCLUSION T-VEC-pembrolizumab did not significantly improve PFS or OS compared with placebo-pembrolizumab. Safety results of the T-VEC-pembrolizumab combination were consistent with the safety profiles of each agent alone.
2D F(R) gravity and AdS2/CFT1correspondence
Authors: Nojiri S. , Odintsov S. D. 
Published: August 2022

Abstract
We studied the canonical structure of 2D F(R) gravity. Its equivalence with Jackiw-Teitelboim gravity is demonstrated when no matter is present. Then, due to AdS2/CFT1 correspondence, such F(R) gravity is equivalent to the Sachdev-Ye-Kitaev models. The singular

limit of F(R) gravity is also studied. It is shown that in such a limit AdS2/CFT1 correspondence is not realized.
The role of syntax in the formation of scale-free language networks
Authors: Yang Mu , Liu Haitao 
Published: August 2022

AbstractThe overall structure of a network is determined by its micro features, which are different in both syntactic and non-syntactic networks. However, the fact that most language networks are small-world and scale-free raises the question: does syntax play a role in forming the scale-free feature? To answer this question, we build syntactic networks and co-occurrence networks to compare the generation mechanisms of nodes, and to investigate whether syntactic and non-syntactic factors have distinct roles. The results show that frequency is the foundation of the scale-free feature, while syntax is beneficial to enhance this feature. This research introduces a microscopic approach, which may shed light on the scale-free feature of language networks.
The Androgen Metabolome of Preterm Infants Reflects Fetal Adrenal Gland Involution
Authors: Flück Christa E , Kuiri-Hänninen Tanja , Silvennoinen Sanna , Sankilampi Ulla , Groessl Michael 
Published: August 2022

Abstract

Context
The human adrenal cortex changes with fetal-neonatal transition from the fetal to the adult organ, accompanied by changes in the steroid metabolome.

Objective
As it is unclear how the observed developmental changes differ between preterm and full-term neonates, we investigated whether the involution of the fetal adrenals is following a fixed time course related to postmenstrual age or whether it is triggered by birth. Furthermore, the fetal and postnatal androgen metabolome of preterm infants was characterized in comparison to term babies.

Methods
This was a prospective, longitudinal, 2-center study collecting spot urines of preterm and term infants during the first 12 to 18 months of life. Steroid metabolites were measured from spot urines by gas chromatography–mass spectrometry. Data relating were modeled according to established pre- and postnatal pathways.

Results
Fetal adrenal involution occurs around term-equivalent age in preterm infants and is not triggered by premature birth. Testosterone levels are higher in preterm infants at birth and decline slower until term compared to full-term babies. Dihydrotestosterone levels and the activity of the classic androgen biosynthesis pathway are lower in premature infants as is 5α-reductase activity. No difference was found in the activity of the alternate backdoor pathway for androgen synthesis.

Conclusion
Human adrenal involution follows a strict timing that is not affected by premature birth. By contrast, prematurity is associated with an altered androgen metabolome after birth. Whether this reflects altered androgen biosynthesis in utero remains to be investigated.
Breasi-CRISPR: an efficient genome-editing method to interrogate protein localization and protein–protein interactions in the embryonic mouse cortex
Authors: Meyerink Brandon L. , KC Pratiksha , Tiwari Neeraj K. , Kittock Claire M. , Klein Abigail , Evans Claire M. , Pilaz Louis-Jan 
Published: August 2022

ABSTRACT
In developing tissues, knowing the localization and interactors of proteins of interest is key to understanding their function. Here, we describe the Breasi-CRISPR approach (Brain Easi-CRISPR), combining Easi-CRISPR with in utero electroporation to tag endogenous proteins within embryonic mouse brains. Breasi-CRISPR enables knock-in of both short and long epitope tag sequences with high efficiency. We visualized epitope-tagged proteins with varied expression levels, such as ACTB, LMNB1, EMD, FMRP, NOTCH1 and RPL22. Detection was possible by immunohistochemistry as soon as 1 day after electroporation and we observed efficient gene editing in up to 50% of electroporated cells. Moreover, tagged proteins could be detected by immunoblotting in lysates from individual cortices. Next, we demonstrated that Breasi-CRISPR enables the tagging of proteins with fluorophores, allowing visualization of endogenous proteins by live imaging in organotypic brain slices. Finally, we used Breasi-CRISPR to perform co-immunoprecipitation mass-spectrometry analyses of the autism-related protein FMRP to discover its interactome in the embryonic cortex. Together, these data demonstrate that Breasi-CRISPR is a powerful tool with diverse applications that will propel the understanding of protein function in neurodevelopment.
Biomechanics of the jaws of spotted ratfish
Authors: Herbert Amanda M. , Dean Mason N. , Summers Adam P. , Wilga Cheryl D. 
Published: August 2022

ABSTRACTElasmobranch fishes (sharks, skates and rays) consume prey of a variety of sizes and properties, and the feeding mechanism typically reflects diet. Spotted ratfish, Hydrolagus colliei (Holocephali, sister group of elasmobranchs), consume both hard and soft prey; however, the morphology of the jaws does not reflect the characteristics typical of durophagous elasmobranchs. This study investigated the mechanical properties and morphological characteristics of the jaws of spotted ratfish over ontogeny, including strain, stiffness and second moment of area, to evaluate the biomechanical function of the feeding structures. Compressive stiffness of the jaws (E=13.51–21.48 MPa) is similar to that of silicone rubber, a very flexible material. In Holocephali, the upper jaw is fused to the cranium; we show that this fusion reduces deformation experienced by the upper jaw during feeding. The lower jaw resists bending primarily in the posterior half of the jaw, which occludes with the region of the upper jaw that is wider and flatter, thus potentially providing an ideal location for the lower jaw to crush or crack prey. The mechanical properties and morphology of the feeding apparatus of spotted ratfish suggest that while the low compressive stiffness is a material limit of the jaw cartilage, spotted ratfish, and perhaps all holocephalans, evolved structural solutions (i.e. fused upper jaw, shape variation along lower jaw) to meet the demands of a durophagous diet.