bluuebunny/crossref_metadata_2025_split huggingface dataset:
RESULTS of MORPHOLOGICAL ANALYSIS of ANIMAL HARD TISSUES IN NORMAL AND SIMULATED OSTEOPOROSIS USING A NON-INVASIVE COMPUTED MICROTOMOGRAPHY TECHNIQUE
Authors: Dolgalev A. , Rzhepakovsky I. , Danaev A. , Avanisyan V. , Shulga G. , Korobkeev A. 
Published: September 2022

Introduction. X-ray microtomography is a non-destructive method of microstructural analysis, which has a high level of detail and allows the possibility of assessing the internal architecture of organs and tissues using 3D-analysis[1]. The speciﬁcs of working with such equipment can be divided into in vivo and in vitro, i.e. working with live laboratory animals (mice, rats, rabbits) under anesthesia or studying organs and tissues separated from the animal [2].The aim of the work was to study the microstructure of sheep bone tissues in normal and simulated osteoporosis using computed microtomography.Materials and methods. We performed microCT analysis of different sheep bones in normal and experimental osteoporosis. Bone tissue of the jaw, iliac and femur, and teeth were collected from control and experimental animals. Bone tissue samples were ﬁxed in 10% buffered formalin. X-ray microCT scanner Skyscan 1176 (BrukermicroCT, Belgium) and software Skyscan 1176 control program (10.0.0.0), Nrecon (1.7.4.2), DataViewer (1.5.6.2), CT-analyser (1.18.4.0), CTvox (3.3.0r1403) were used to scan and process materials.Results. MicroCT examination and 3D-imaging conﬁrmed the elimination of trabeculae in the metaphyseal region of the femur in sheep with experimental osteoporosis from the centre to the periphery; in addition, 3D-analysis showed a 15.1% decrease in bone percentage, a 7.8% decrease in bone mineral density, and an increase in Tb. Sp. (trabecular separation), Tb. Pf. (trabecular pattern factor) and SMI (structure model index) by 30.2%, 20.8% and 23.6%, respectively, and a decrease in Tb.N. (trabecular number) index by 18.6%, indicating calcium washout, decreased trabecular connectivity and a transition from a lamellar to a rod-like architecture. Similar changes were found in the 3D-analysis of the jaw bone tissue. Thus, a decrease of 18.9% in mineral density was found, as well as a signiﬁcant increase of 11.58 and 2.21 in the indices, particularly Tb. Pf. and SMI. 3D-analysis of iliac microtomography also indicates a simulation of osteoporosis, as evidenced by a signiﬁcant increase in the main indices characterising the development of this pathology.Conclusions. The obtained results not only objectively testify to the development of osteoporosis in the experimental animals, but also indicate signs of the adaptation-compensatory reactions of the body, characterized by appearance of large single trabeculae in the metaphysis of the femur as well as by not expressed reduction of bone mineral density and bone tissue area.
TISSUE-ENGINEERED BONE IMPLANTS FOR THE REPLACEMENT OF JAWBONE DEFECTS. LITERATURE REVIEW
Authors: Kobets K. , Kazaryan A. , Bopkhoev S. 
Published: September 2022

The purpose of the study:to trace the development of methods of bone implants for the replacement of jawbone defects: from ceramic and polymeric scaffolds to complex tissue-engineered structures with stem cells, growth factors and vascular anastomoses based on literature data.Materials and methods:searching, systematization and analysis of scientiﬁc data on various types of 3D-printed bone implants and their effectiveness in replacing bone defects.Conclusions:Modern technologies of 3D-printing, cell and tissue engineering, microvascular surgical techniques closely approach scientists and clinicians to creation of an artiﬁcial bone implant which in the body must become a living structure capable of integrating with the patient’s bone. Only complex approach which includes reconstruction of the implant of individual shape and sufﬁcient mechanical strength, giving of osteoinductive and osteogenic properties, providing of internal axial and external angiogenesis is the basis for such tissue-engineered construction.
THE EFFECT of HIGH-INTENSITY INTERVAL PHYSICAL TRAINING ON THE ENERGY SUPPLY SYSTEM IN PATIENTS AFTER HEART SURGERY
Authors: Kuular A. , Kakuchaya T. , Dzhitava T. , Filatova A. , Pachuashvili N. 
Published: September 2022

Introduction. A number of studies have found a reduction in the risk of rehospitalization during cardiorehabilitation after heart surgery. The purpose of the training program for cardiorehabilitation after cardiac surgery is to optimize energy supply systems. Depending on the intensity and duration of the load being performed, energy supply systems 'switch on' in the body.Purpose of the study: to study the effectiveness of the optimal cardiorehabilitation training program on the aerobic energy supply system in cardiac surgery patients.Materials and methods. In our study, we examined 47 patients who underwent aortic valve replacement under cardiopulmonary bypass, with an EC duration of 52±20.5 minutes. The patients were divided into two groups: the control group (n=23), who underwent a course of cardiorehabilitation on exercise bikes with constant aerobic training of moderate and medium intensity, at the age of 50.2±3.4 years, and the main group (n=24) – with a course of cardiorehabilitation on exercise bikes with aerobic high-intensity interval physical training, at the age of 51.2±2.8 years (p>0.05). Gender was not taken into account. Anaerobic training was carried out on SCHILLER exercise bikes from day 14 after aortic valve replacement. The load power ranged from 25 to 70–95 watts. Before cardiac rehabilitation, the level of lactate, glucose, pH, pCO2, pO2, BE, lipid proﬁle, BNP in the blood before and after ergometric exercise, data on external respiration, heart rate and blood pressure were assessed. After the course of rehabilitation, the above parameters were evaluated. The duration of the course was 2 weeks. Results. When evaluating the indicators at the end of the course of cardiorehabilitation was being done, a signiﬁcant decrease in the controlled parameters after training was observed in both groups, and largely in the group of high-intensity interval physical training than in the group of constant training of medium intensity.Conclusions. Analysis of the results showed that properly selected parameters of high-intensity interval training could optimize anaerobic glycolysis in patients undergoing operations under cardiopulmonary bypass, as well as increase the functionality of patients. As more and more repetitions of the load are performed, interval training becomes more and more aerobic.
Oblique impact responses of Hybrid III and a new headform with more biofidelic coefficient of friction and moments of inertia
Authors: Yu Xiancheng , Halldin Peter , Ghajari Mazdak 
Published: September 2022

New oblique impact methods for evaluating head injury mitigation effects of helmets are emerging, which mandate measuring both translational and rotational kinematics of the headform. These methods need headforms with biofidelic mass, moments of inertia (MoIs), and coefficient of friction (CoF). To fulfill this need, working group 11 of the European standardization head protection committee (CEN/TC158) has been working on the development of a new headform with realistic MoIs and CoF, based on recent biomechanics research on the human head. In this study, we used a version of this headform (Cellbond) to test a motorcycle helmet under the oblique impact at 8 m/s at five different locations. We also used the Hybrid III headform, which is commonly used in the helmet oblique impact. We tested whether there is a difference between the predictions of the headforms in terms of injury metrics based on head kinematics, including peak translational and rotational acceleration, peak rotational velocity, and BrIC (brain injury criterion). We also used the Imperial College finite element model of the human head to predict the strain and strain rate across the brain and tested whether there is a difference between the headforms in terms of the predicted strain and strain rate. We found that the Cellbond headform produced similar or higher peak translational accelerations depending on the impact location (−3.2% in the front-side impact to 24.3% in the rear impact). The Cellbond headform, however, produced significantly lower peak rotational acceleration (−41.8% in a rear impact to −62.7% in a side impact), peak rotational velocity (−29.5% in a side impact to −47.6% in a rear impact), and BrIC (−29% in a rear-side impact to −45.3% in a rear impact). The 90th percentile values of the maximum brain strain and strain rate were also significantly lower using this headform. Our results suggest that MoIs and CoF have significant effects on headform rotational kinematics, and consequently brain deformation, during the helmeted oblique impact. Future helmet standards and rating methods should use headforms with realistic MoIs and CoF (e.g., the Cellbond headform) to ensure more accurate representation of the head in laboratory impact tests.
Biomechanical comparison between unilateral and bilateral percutaneous vertebroplasty for osteoporotic vertebral compression fractures: A finite element analysis
Authors: Dai Haowen , Liu Yang , Han Qing , Zhang Aobo , Chen Hao , Qu Yang , Wang Jincheng , Zhao Jianwu 
Published: September 2022

Background and objective: The osteoporotic vertebral compression fracture (OVCF) has an incidence of 7.8/1000 person-years at 55–65 years. At 75 years or older, the incidence increases to 19.6/1000 person-years in females and 5.2–9.3/1000 person-years in males. To solve this problem, percutaneous vertebroplasty (PVP) was developed in recent years and has been widely used in clinical practice to treat OVCF. Are the clinical effects of unilateral percutaneous vertebroplasty (UPVP) and bilateral percutaneous vertebroplasty (BPVP) the same? The purpose of this study was to compare biomechanical differences between UPVP and BPVP using finite element analysis.Materials and methods: The heterogeneous assignment finite element (FE) model of T11-L1 was constructed and validated. A compression fracture of the vertebral body was performed at T12. UPVP and BPVP were simulated by the difference in the distribution of bone cement in T12. Stress distributions and maximum von Mises stresses of vertebrae and intervertebral discs were compared. The rate of change of maximum displacement between UPVP and BPVP was evaluated.Results: There were no obvious high-stress concentration regions on the anterior and middle columns of the T12 vertebral body in BPVP. Compared with UPVP, the maximum stress on T11 in BPVP was lower under left/right lateral bending, and the maximum stress on L1 was lower under all loading conditions. For the T12-L1 intervertebral disc, the maximum stress of BPVP was less than that of UPVP. The maximum displacement of T12 after BPVP was less than that after UPVP under the six loading conditions.Conclusion: BPVP could balance the stress of the vertebral body, reduce the maximum stress of the intervertebral disc, and offer advantages in terms of stability compared with UPVP. In summary, BPVP could reduce the incidence of postoperative complications and provide promising clinical effects for patients.
Easily automated radiosynthesis of [18F]P10A-1910 and its clinical translation to quantify phosphodiesterase 10A in human brain
Authors: Wei Huiyi , Wei Junjie , Zhang Shaojuan , Dong Shiliang , Li Guocong , Ran Wenqing , Dong Chenchen , Zhang Weibin , Che Chao , Luo Wenzhao , Xu Hao , Dong Zhiyong , Wang Jinghao , Wang Lu 
Published: September 2022

Our previous work showed that [18F]P10A-1910 was a potential radioligand for use in imaging phosphodiesterase 10A (PDE10A). Specifically, it had high brain penetration and specific binding that was demonstrated in both rodents and non-human primates. Here, we present the first automatic cGMP-level production of [18F]P10A-1910 and translational PET/MRI study in living human brains. Successful one-step radiolabeling of [18F]P10A-1910 on a GE TRACERlab FX2N synthesis module was realized via two different methods. First, formulated [18F]P10A-1910 was derived from heating spirocyclic iodonium ylide in a tetra-n-butyl ammonium methanesulfonate solution. At the end of synthesis, it was obtained in non-decay corrected radiochemical yields (n.d.c. RCYs) of 12.4 ± 1.3%, with molar activities (MAs) of 90.3 ± 12.6 μmol (n = 7) (Method I). The boronic pinacol ester combined with copper and oxygen also delivered the radioligand with 16.8 ± 1.0% n. d.c. RCYs and 77.3 ± 20.7 GBq/μmol (n = 7) MAs after formulation (Method II). The radiochemical purity, radionuclidic purity, solvent residue, sterility, endotoxin content and other parameters were all validated for human use. Consistent with the distribution of PDE10A in the brain, escalating uptake of [18F]P10A-1910 was observed in the order of cerebellum (reference region), substantial nigra, caudate and putamen. The non-displaceable binding potential (BPND) was estimated by simplified reference-tissue model (SRTM); linear regressions demonstrated that BPND was well correlated with the most widely used semiquantitative parameter SUV. The strongest correlation was observed with SUV(50–60 min) (R2 = 0.966, p &lt; 0.01). Collectively, these results indicated that a static scan protocol could be easily performed for PET imaging of PDE10A. Most importantly, that [18F]P10A-1910 is a promising radioligand to clinically quantify PDE10A.
Higher neutrophil to lymphocyte ratio is associated with renal dysfunction and cardiac adverse remodeling in elderly with metabolic syndrome
Authors: Zhu Yuqi , Li Gang , Laukkanen Jari A. , Song Xing , Zhang Jing , Wei Linping , Chen Xinrui , Li Yufeng , Liu Cheng 
Published: September 2022

BackgroundPrevious studies have shown that metabolic syndrome (MetS) is associated with increased systemic inflammation and cardiac mortality in elderly subjects. However, information on the association of inflammation markers with cardiac adverse remodeling is limited in the elderly with MetS. Therefore, we investigated whether the inflammatory marker neutrophil/lymphocyte ratio (NLR) is associated with the cardiac adverse remodeling in Chinese elderly with MetS.MethodsA total of 1,087 hospitalized Chinese elderly (aged ≥ 65 years) with MetS were collected retrospectively. The cross-sectional data of echocardiography and clinical parameters were compared among quartile NLR groups.ResultsIn the elderly with MetS, higher quartile NLR (≥3.83) was found to be associated with male gender, older age, lower estimated glomerular filtration rate (eGFR), and cardiac left ventricular (LV) dilatation (all p &lt;0.05).ConclusionHigher NLR is associated with male gender, older age, renal dysfunction, and cardiac adverse remodeling in Chinese elderly with MetS.
Identification of biomarkers and analysis of infiltrated immune cells in stable and ruptured abdominal aortic aneurysms
Authors: Chen Yubin , Ouyang Tianyu , Fang Cheng , Tang Can-e , Lei Kaibo , Jiang Longtan , Luo Fanyan 
Published: September 2022

ObjectivesThe mortality rate of abdominal aortic aneurysm (AAA) is extremely high in the older population. This study aimed to identify potential biomarkers of AAA and aortic rupture and analyze infiltration of immune cells in stable and ruptured AAA samples.MethodsRaw data of GSE47472, GSE57691, and GSE98278 were downloaded. After data processing, the co-expression gene networks were constructed. Gene Ontology and pathway enrichment analysis of AAA- and aortic rupture-related gene modules were conducted using the Database for Annotation, Visualization, and Integrated Discovery. Gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) were used for further enrichment analysis. The CIBERSORT tool was used to analyze the relative abundance of immune cells in samples. Differentially expressed immune-related genes were analyzed between different samples. Predictive models were constructed via extreme gradient boosting, and hub genes were identified according to feature importance.ResultsBlue and yellow modules were significantly related to AAA, and genes in these modules were associated with the aortic wall and immune response, respectively. In terms of aortic rupture, the most relevant module was significantly enriched in the inflammatory response. The results of GSEA and GSVA suggested that immune cells and the inflammatory response were involved in the development of AAA and aortic rupture. There were significant differences in the infiltration of immune cells and expression levels of immune-related genes among different samples. NFKB1 might be an important transcription factor mediating the inflammatory response of AAA and aortic rupture. After the construction of a predictive model, CD19, SELL, and CCR7 were selected as hub genes for AAA whereas OAS3, IFIT1, and IFI44L were identified as hub genes for aortic rupture.ConclusionWeakening of the aortic wall and the immune response both contributed to the development of AAA, and the inflammatory response was closely associated with aortic rupture. The infiltration of immune cells was significantly different between different samples. NFKB1 might be an important transcription factor in AAA and aortic rupture. CD19, SELL, and CCR7 had potential diagnostic value for AAA. OAS3, IFIT1, and IFI44L might be predictive factors for aortic rupture.
SGLT-2 inhibitors on prognosis and health-related quality of life in patients with heart failure and preserved ejection fraction: A systematic review and meta-analysis
Authors: Yang Danning , Zhang Yu , Yan Jie , Liu Ming , An Fengshuang 
Published: September 2022

BackgroundHeart failure with preserved ejection fraction (HFpEF) is becoming the main subtype of heart failure, but lacks proven effective therapies. Sodium-glucose cotransporter-2 (SGLT-2) inhibitor, a new kind of oral glucose-lowering agent, shows a great effect on improving cardiovascular outcomes. Based on the results of current RCTs, we perform this meta-analysis to illustrate the therapeutic impact of SGLT2i in HFpEF patients.MethodsWe systematically searched the online database and 10 RCTs were involved. The primary outcome was the prognosis outcome of HFpEF patients, including a composite outcome of cardiovascular (CV) death and hospitalization for heart failure (HHF), CV mortality, HHF, and all-cause mortality. Main secondary outcomes included improvement of KCCQ-TSS (Kansas City Cardiomyopathy Questionnaire and total symptom score) and 6-Minute Walk Test (6MWT). All pooled results were calculated by the random-effects model. Statistical heterogeneity was assessed using the chi-squared test and was quantified using the I-squared statistic.ResultsTen RCTs comprising 10,334 patients were involved in. Incidence of composite outcome was reduced in SGLT-2 inhibitor group compared with placebo (HR: 0.78, 95% CI: 0.69–0.88, p = 0.00). Improvement of KCCQ-TSS was also more pronounced in the SGLT-2 inhibitor group (MD: 2.74, 95% CI: 1.30–4.18, p = 0.00). No statistical difference was observed in 6MWT.ConclusionTreating HFpEF patients with SGLT-2 inhibitors is associated with reducing the composite outcome of CV death and HHF and improving health-related quality of life. Further studies with more evidence are in need to confirm this conclusion.
Association between coronary plaque volume and myocardial ischemia detected by dynamic perfusion CT imaging
Authors: Vattay Borbála , Borzsák Sarolta , Boussoussou Melinda , Vecsey-Nagy Milán , Jermendy Ádám L. , Suhai Ferenc I. , Maurovich-Horvat Pál , Merkely Béla , Kolossváry Márton , Szilveszter Bálint 
Published: September 2022

IntroductionWe aimed to evaluate the relationship between quantitative plaque metrics derived from coronary CT angiography (CTA) and segmental myocardial ischemia using dynamic perfusion CT (DPCT).MethodsIn a prospective single-center study, patients with &gt; 30% stenosis on rest CTA underwent regadenoson stress DPCT. 480 myocardium segments of 30 patients were analyzed. Quantitative plaque assessment included total plaque volume (PV), area stenosis, and remodeling index (RI). High-risk plaque (HRP) was defined as low-attenuation plaque burden &gt; 4% or RI &gt; 1.1. Absolute myocardial blood flow (MBF) and relative MBF (MBFi: MBF/75th percentile of all MBF values) were quantified. Linear and logistic mixed models correcting for intra-patient clustering and clinical factors were used to evaluate the association between total PV, area stenosis, HRP and MBF or myocardial ischemia (MBF &lt; 101 ml/100 g/min).ResultsMedian MBF and MBFi were 111 ml/100 g/min and 0.94, respectively. The number of ischemic segments were 164/480 (34.2%). Total PV of all feeding vessels of a given myocardial territory differed significantly between ischemic and non-ischemic myocardial segments (p = 0.001). Area stenosis and HRP features were not linked to MBF or MBFi (all p &gt; 0.05). Increase in PV led to reduced MBF and MBFi after adjusting for risk factors including hypertension, diabetes, and statin use (per 10 mm3; β = −0.035, p &lt; 0.01 for MBF; β = −0.0002, p &lt; 0.01 for MBFi). Similarly, using multivariate logistic regression total PV was associated with ischemia (OR = 1.01, p = 0.033; per 10 mm3) after adjustments for clinical risk factors, area stenosis and HRP.ConclusionTotal PV was independently associated with myocardial ischemia based on MBF, while area stenosis and HRP were not.
Study on structure optimization and applicability of hydrocyclone in natural gas hydrate exploitation
Authors: Wei Na , Qiao Yi , Liu Anqi , Zhao Jinzhou , Zhang Liehui , Xue Jin 
Published: September 2022

With the development of economy and industry, the consumption of fossil energy is gradually increasing. Currently, natural gas hydrates (NGH) are considered to be an ideal alternative energy due to its large reserve and high energy density. However, the separation of hydrate slurry is a critical step in the exploitation of NGH. In this study, the optimization of the structural parameters based on the conventional three-phase hydrocyclone was carried out using numerical simulation and orthogonal design. At the same time, the separation efficiency criterion E was defined to evaluate the separation efficiency by calculating the sum of the discharge efficiency of water and gas. Analysis of the effect of individual structural factors on the flow field distribution characteristics inside the hydrocyclone using the single factor analysis method. The results showed that: with the combination of structural parameters of s was 2 mm2 × 12 mm2, dz was 28 mm, h was 20 mm, and li was 28 mm, the maximum E of the three-phase hydrocyclone was 1.46. The effect significance of each structural parameter on the separation efficiency from the highest to the lowest was the s, dz, h, and li. In the single-factor analysis, when the s was 2 mm2 × 12 mm2, the tangential velocity, axial velocity, and CH4 volume fraction in the flow field reached the maximum of 13.65 m/s, 4.35 m/s, and 12.4%, respectively. But the minimum water phase volume fraction was 69.9% under this structural parameter condition. When the dz was 36 mm, the maximum decrease value of axial velocity in the flow field was 3.57 m/s, the maximum CH4 volume fraction was 12.4%, and the water volume fraction reached a minimum of 66.3%. The li and the h only had significant effects on the axial velocity and the water volume fraction, respectively. The axial velocity reached a maximum of 4.75 m/s when the li was 32 mm, and the water phase volume fraction reached a maximum of 77.9% when the h was 10 mm. The study of the structural parameters and flow field characteristics of this three-phase hydrocyclone applicable to the solid fluidization exploitation of NGH provides some guidance and suggestions for the separation of hydrate slurry after solid fluidization exploitation of NGH.