bluuebunny/crossref_metadata_2025_split huggingface dataset:
Will the PRESERFLO™ MicroShunt impact the future of trabeculectomy practice? A UK and Éire Glaucoma Society National Survey
Authors: Kuet Mong-Loon , Azuara-Blanco Augusto , Barton Keith , King Anthony J. 
Published: December 2022

Abstract
Background/objectives
To explore the attitudes of UK glaucoma specialists regarding the current and future practice of trabeculectomy and the novel PRESERFLO™ MicroShunt (PF-MS) device, and intentions to adopt the PF-MS into routine glaucoma surgical practice.

Methods
Online survey of UK and Éire Glaucoma Society members.

Results
43 glaucoma consultants completed the survey. All surgeons performed trabeculectomies (median of 40 annually) and 51% undertook PF-MS procedures (median of 22.5 annually). The mean duration of surgery was reported as 48.9 (SD 13.3) and 31.2 (SD 9.9) min for trabeculectomy and PF-MS respectively (p < 0.0001). For surgeons not currently using the PF-MS, 65% planned to do so. Respondents judged completion of 35 trabeculectomies and 10 PF-MS were required to achieve basic competence. 91% of participants predicted their trabeculectomy volume would decrease and 73% expected PF-MS usage to increase. Respondents reported a median of six and four follow-ups within 3 months post surgery for trabeculectomy and PF-MS respectively (p < 0.0001). Respondents reported trabeculectomy required more post-operative interventions than the PF-MS and 81.8% judged the patient experience to be better with the PF-MS. The PF-MS was deemed suitable for early visual field loss by 72% of respondents, severe visual field loss by 35% and normal tension glaucoma by 21%.

Conclusion
The PF-MS has seen rapid adoption in the UK. Respondents predict its usage will significantly increase whilst trabeculectomies will decrease. They report the PF-MS is quicker to learn and perform, and requires less post-operative follow-ups and interventions which may facilitate a more efficient service delivery for patients requiring glaucoma surgery.
Principles of mitoribosomal small subunit assembly in eukaryotes
Authors: Harper Nathan J. , Burnside Chloe , Klinge Sebastian 
Published: December 2022

AbstractMitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism1. Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies.
A critical period of translational control during brain development at codon resolution
Authors: Harnett Dermot , Ambrozkiewicz Mateusz C. , Zinnall Ulrike , Rusanova Alexandra , Borisova Ekaterina , Drescher Amelie N. , Couce-Iglesias Marta , Villamil Gabriel , Dannenberg Rike , Imami Koshi , Münster-Wandowski Agnieszka , Fauler Beatrix , Mielke Thorsten , Selbach Matthias , Landthaler Markus , Spahn Christian M. T. , Tarabykin Victor , Ohler Uwe , Kraushar Matthew L. 
Published: December 2022

AbstractTranslation modulates the timing and amplification of gene expression after transcription. Brain development requires uniquely complex gene expression patterns, but large-scale measurements of translation directly in the prenatal brain are lacking. We measure the reactants, synthesis and products of mRNA translation spanning mouse neocortex neurogenesis, and discover a transient window of dynamic regulation at mid-gestation. Timed translation upregulation of chromatin-binding proteins like Satb2, which is essential for neuronal subtype differentiation, restricts protein expression in neuronal lineages despite broad transcriptional priming in progenitors. In contrast, translation downregulation of ribosomal proteins sharply decreases ribosome biogenesis, coinciding with a major shift in protein synthesis dynamics at mid-gestation. Changing activity of eIF4EBP1, a direct inhibitor of ribosome biogenesis, is concurrent with ribosome downregulation and affects neurogenesis of the Satb2 lineage. Thus, the molecular logic of brain development includes the refinement of transcriptional programs by translation. Modeling of the developmental neocortex translatome is provided as an open-source searchable resource at https://shiny.mdc-berlin.de/cortexomics.
Gum Arabic nanoformulation rescues neuronal lesions in bromobenzene-challenged rats by its antioxidant, anti-apoptotic and cytoprotective potentials
Authors: Almohaimeed Hailah M. , Waly Hanan , Abou Khalil Nasser S. , Hassanein Khaled M. A. , Alkhudhairy Basal Sulaiman M. , Abd-Allah Elham A. 
Published: December 2022

AbstractBromobenzene (BB) is a hazardous environmental contaminant because of its multiple routes of exposure and the toxicity of its bio-derivates. It could elicit neuronal alterations by stimulating redox imbalance and apoptotic pathways. Gum Arabic (GA) protected the hippocampus of a type 2 diabetic rat model from cognitive decline. Whether gum Arabic nanoemulsion (GANE) can increase the neuroprotectant potency of GA in fighting BB-associated neurological lesions is the question to be answered. To accomplish this objective, 25 adult male Wistar rats were randomly and equally assigned into five groups. Control received olive oil (vehicle of BB). BB group received BB at a dose of 460 mg/kg BW. Blank nanoemulsion (BNE) group supplemented with BNE at 2 mL of 10% w/v aqueous suspension/kg BW. GANE group received GANE at a dose of 2 mL of 10% w/v aqueous suspension/kg BW. BB + GANE group exposed to BB in concomitant with GANE at the same previous doses. All interventions were carried out daily by oral gavage for ten consecutive days. BB caused a marked increase in malondialdehyde and succinate dehydrogenase together with a marked decrease in reduced glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase, and lactate dehydrogenase in the brain. BB was accompanied by pathological deteriorations, amyloidosis, and reduced immuno-expression of integrase interactor 1 in the hippocampal region. Administration of GANE was beneficial in reversing the aforementioned abnormalities. These results pave the road for further discovery of nano-formulated natural products to counter the threats of BB.
Synthesize of Bi2O3/Gln-TSC nanoparticles and evaluation of their toxicity on prostate cancer cells and expression of CASP8, BAX, and Bcl-2 genes
Authors: Moradi Asal , Abdihaji Mohammadreza , Kouchaksaraie Sara Barari , Alkinani Tabarek Abdulrazaq , Mahmoudi Aida , Davoudi Arash , Dashtmiani William , Ghezeljeh Somayeh Mikaeili , Aghajani Shahrzad , Ghasemian Reza , Taramsari Somayeh Maghsoomi , Majlesi Amitis , Niyaki Zahra Mahdavi , Salehzadeh Ali 
Published: December 2022

AbstractDue to the high prevalence and considerable increase of prostate cancer, finding novel therapeutic compounds for the treatment of prostatic cancer has been the goal of many researches. In this study, we aimed to fabricate the Bismuth oxide (Bi2O3) NPs, functionalized with glutamine (Gln) and conjugated with Thiosemicarbazide (TSC). Then, the anticancer mechanism of the synthesized NPs was investigated using the cellular and molecular tests including MTT assay, Flow cytometry, Caspase-3 activity assay, Hoechst staining and Real Time PCR. The FT-IR and XRD assays confirmed the identity of the synthesized Bi2O3/Gln-TSC NPs. The size range of the synthesized spherical particles was 10–60 nm and the zeta potential was − 23.8 mV. The purity of the NPs was confirmed by EDX-mapping analysis. The Bi2O3/Gln-TSC was considerably more toxic for prostate cancer cells than normal human cells and the IC50 was calculated 35.4 and 305 µg/mL, respectively. The exposure to the NPs significantly increased the frequency of apoptotic cells from 4.7 to 75.3%. Moreover, the expression of the CASP8, BAX, and Bcl-2 genes after exposure to the NPs increased by 2.8, 2.3, and 1.39 folds. Treating the cancer cells with Bi2O3/Gln-TSC increased the activity of the Caspase-3 protein and apoptotic morphological features were observed by Hoechst staining in the treated cells. This work showed that Bi2O3/Gln-TSC has considerable cytotoxicity for prostate cancer cells and could inducing both intrinsic and extrinsic pathways of apoptosis.
Epigenetic age provides insight into tissue origin in endometriosis
Authors: Leap Katie , Yotova Iveta , Horvath Steve , Martinez-Agosto Julian A. 
Published: December 2022

AbstractEndometriosis is a common reproductive disease with a heterogeneous presentation. Classification attempts have thus far not offered insight into its cause or its symptoms. Endometriosis may result from the migration of shed endometrium to the peritoneal cavity. However, there are cases reported in girls without uteruses and men. While a non-retrograde menstruation origin of ectopic tissue is certain in these cases, we explored the use of DNA methylation age (DNAm age) to distinguish between retrograde and non-retrograde tissue origin in endometriosis. Using publicly available DNA methylation data and Horvath’s pan-tissue epigenetic clock, we compared DNAm age and epigenetic age acceleration (EAA) of ectopic lesions to eutopic endometrium of diseased and control endometrium. We examined EAA in cancer metastasis and teratomas to control for migration and developmental origin. Disease status does not change DNAm age of eutopic endometrium, but the effect of ectopic status was profound: − 16.88 years (p = 4.82 × 10–7). There were no differences between EAA of primary/metastatic tumor paired samples, suggesting that the observed effect is not due to tissue migration or ectopic location. Immature or mature teratoma compartments decreased DNAm age by 9.44 and 7.40 years respectively, suggesting that developmental state correlates with DNAm age. Ectopic endometriotic tissue exhibits decelerated DNAm age, similar to that observed in teratomas composed of multipotent tissue, but distinct from eutopic tissue. The migration process does not change DNAm age and eutopic endometrium is concordant with chronological age regardless of disease status. We conclude that DNAm age of ectopic lesions suggests a distinct developmental origin for a subset of lesions. This finding may assist in classifying endometriosis into distinct subtypes that may be clinically relevant.
Increased risk of cataract surgery in patients with allergic disease: a population based cohort study
Authors: Paik Ji-Sun , Han Kyungdo , Nam Gahee , Park Sun-Kyoung , Hwang Ho Sik , Chun Yoon Hong , Na Kyung-Sun 
Published: December 2022

AbstractWe investigated the association between cataract and allergic diseases, including atopic dermatitis (AD), allergic rhinitis (AR), and asthma using 2,631,015 subjects’ data from the 2009 National Health Insurance Service-Health Screening Cohort in Korea. Each allergic disease was defined as three or more occasions of diagnosis within 1 year with dedicated ICD-10 codes. The primary endpoint was newly received cataract surgery during the follow-up period. In total, 447,883 subjects had at least one allergic disease. During the mean follow-up of 7.8 ± 1.7 years, newly developed cataract surgery was observed in 301,693 subjects (allergic group, n = 69,321; non-allergic group, n = 232,372). After adjusting for demographic characteristics (age, sex), systemic and ocular comorbidities, socioeconomic status, and lifestyle factors (smoking, drinking, regular exercise), the allergic group had a higher hazard ratio (HR) for cataract development compared with the non-allergic group. We further performed a subgroup analysis for patients regarding sex and age. In the subgroup analysis of subjects with AD, men aged < 50 years had a higher HR compared to women of the same age group. In conclusion, subjects with allergic diseases had a higher risk of cataract surgery than their counterparts, and the combination of AD and AR resulted in the highest risk. Particularly, the association was more evident in male than female patients with AD aged < 50 years.
Intensified modulation of the Pacific north equatorial current bifurcation by the southern annular mode since the early 1990s
Authors: Wang Li-Chiao , Lin Yong-Fu , Wu Chau-Ron 
Published: December 2022

AbstractLong-term reanalysis data were used to assess inter-decadal to decadal modulations of the North Equatorial Current (NEC) bifurcation in the Pacific after the early 1990s. The wind stress curl anomaly (WSCA) in the region of 10° N–15° N and 160° E–170° E (C-BOX) had been found to excite Rossby waves and control NEC bifurcation along the Philippine coast. Our analysis revealed that the WSCA in the C-BOX has been remotely modulated by the Southern Annular Mode (SAM) since the early 1990s. It is shown that the SAM shifted to its positive phase at this transition and began strongly impacting the WSCA in the C-BOX and the NEC bifurcation. During the positive SAM phase after the early 1990s, strong climate variability occurred in the tropical to subtropical area of the North Pacific, with a clear footprint connected to the Antarctic region. Consistent with that finding, we determined that during the positive SAM phase, a dipole sea surface temperature pattern was generated in the South Pacific; this induced an atmospheric Rossby wave train in upper-level wind shear that propagated northward to the North Pacific. Such effects further enhanced downward motion and divergence at the surface, intensifying the easterlies in the equatorial area and the anticyclonic WSCA in the C-BOX. The anticyclonic WSCA in the C-BOX substantially excited downwelling oceanic Rossby waves at the surface, inducing an equatorward trend of NEC bifurcation after the early 1990s.
Surface modification of thin film composite forward osmosis membrane using graphene nanosheets for water desalination
Authors: El-Sayed Fatma Mohamed , E.A.Ali Mohamed , Isawi Heba , Abo Aly M. M. , Abo El-Fadl M. M. S. 
Published: December 2022

AbstractIn this study, the main motivation of this work is desalination of water for irrigation arid area such as Sidri- Baba basins- south Sinai, Egypt. Also, the novelty of this work is modification of TFC surface membrane by mix of HA, DA and GO to get high performance of FO technique. Interfacial polymerization was employed to modify a thin-film composite (TFC) membrane for forward osmosis (FO) applications; moreover, graphene oxide (GO) nanosheets (GONs), a dopamine solution (DA), and naturally accessible humic acid (HA) were modified on a polyethersulfone (PES) substrate. The effects of the different quantities of GO, HA, and DA on the membrane surfaces, as well as their various cross-sectional morphologies and FO-desalination capabilities, were investigated. The integrated TFC membrane containing appropriate GO, HA, and DA blends outperformed the control membrane, obtaining high water flux, and high salt rejection. Furthermore,.
Effects of pain and depression on the relationship between household solid fuel use and disability among middle-aged and older adults
Authors: Jia Zhihao , Gao Yan , Zhao Liangyu , Han Suyue 
Published: December 2022

AbstractHousehold air pollution (HAP) is suggested to increases people's risk of disability, but mediating mechanisms between HAP and disability remains under-investigated. The aim of this study was to investigate the underlying mechanisms between household air pollution and disability in middle-aged and older adults (i.e., older than 45 years) using a nationally representative prospective cohort. In total, 3754 middle-aged and older adults were selected from the China Health and Retirement Longitudinal Study. Correlation analysis and logistic regression analysis were employed to estimate the association between HAP, pain, depression and disability. Finally, three significant mediation pathways through which HAP directly impacts disability were found: (1) pain (B = 0.09, 95% CI 0.01, 0.02), accounting for 15.25% of the total effect; (2) depression (B = 0.07, 95% CI 0.004, 0.02), accounting for 11.86% of the total effect; (3) pain and depression (B = 0.04, 95% CI 0.003, 0.01), accounting for 6.78% of the total effect. The total mediating effect was 33.89%. This study clarified that HAP can indirectly affect disability through the respective and serial mediating roles of pain and depression. These findings potentially have important implications for national strategies concerning the widespread use of clean fuels by citizens.
Relationships between resting-state EEG functional networks organization and individual differences in mind wandering
Authors: Krukow Paweł , Jonak Kamil 
Published: December 2022

AbstractWhen performing cognitively demanding tasks, people tend to experience momentary distractions or personal associations that intercept their stream of consciousness. This phenomenon is known as Mind Wandering (MW) and it has become a subject of neuroscientific investigations. Off-task thoughts can be analyzed during task performance, but currently, MW is also understood as a dimension of individual differences in cognitive processing. We wanted to recognize the intrinsically-organized functional networks that could be considered the neuronal basis for MW dispositional variability. To achieve this goal we recruited a group of normal adults, and eventually divided the group in half, based on participants’ scores on the scale measuring dispositional MW. Next, these groups were compared regarding the arrangement of preselected intrinsic functional networks, which were reconstructed based on multi-channel signal-source resting-state EEG. It appeared that subjects who tend to mind wander often exhibited decreased synchronization within the default mode network, and, simultaneously, strengthened connectivity between ‘on-task’ networks of diverse functional specificity. Such within- and between networks integrity patterns might suggest that greater Mind Wanderers present an atypical organization of resting-state brain activity, which may translate into attenuated resources needed to maintain attentional control in task-related conditions.
Adaptive sliding mode control strategy based on disturbance observer and neural network for lower limb rehabilitative robot
Authors: Ma Yihang , Wang Jirong , Li Qianying , Shi Lianwen , Qin Yunhao , Liu Huabo , Tian Hongzhi 
Published: December 2022

AbstractIn this study, to achieve accurate tracking of the desired trajectory during passive control of the lower limb rehabilitation robot, an adaptive sliding mode controller based on disturbance observer and radial basis function neural network (RBFNN) is proposed for the lower limb rehabilitative robot in the presence of uncertain parameters and external bounded disturbances. First, the Euler–Lagrange dynamic model of the lower limb rehabilitative robot is described. Second, a sliding mode controller is designed to stabilize the system with an improved sliding mode reach rate under the assumption that all parameters of the dynamics model are known. To achieve a sliding mode controller without the above assumptions, the proposed adaptive RBFNN and the disturbance observers are employed to compensate for disturbances and the uncertainties in the robot's dynamic mode via feedforward loops. The Lyapunov stability theory is used to prove that the proposed controller has accomplished a significant control effect with excellent performance and the output tracking error can be converted to a very small neighborhood through reasonable design parameters. Finally, the performance of the controller based on the state feedback and state observer are demonstrated by numerical simulations, respectively.
A multi‐scale feature representation and interaction network for underwater object detection
Authors: Yuan Jiaojiao , Hu Yongli , Sun Yanfeng , Yin Baocai 
Published: December 2022

AbstractCompared with natural images, underwater images are usually degraded with blur, scale variation, colour shift and texture distortion, which bring much challenge for computer vision tasks like object detection. In this case, generic object detection methods usually fail to achieve satisfactory performance. The main reason is considered that the current methods lack sufficient discriminativeness of feature representation for the degraded underwater images. A a novel multi‐scale feature representation and interaction network for underwater object detection is proposed, in which two core modules are elaborately designed to enhance the discriminativeness of feature representation for underwater images. The first is the Context Integration Module, which extracts rich context information from high‐level features and is integrated with the feature pyramid network to enhance the feature representation in a multi‐scale way. The second is the Dual‐refined Attention Interaction Module, which further enhances the feature representation by sufficient interactions between different levels of features both in channel and spatial domains based on attention mechanism. The proposed model is evaluated on four public underwater datasets. The experimental results compared with state‐of‐the‐art object detection methods show that the proposed model has leading performance, which verifies that it is effective for underwater object detection. In addition, object detection experiments on a foggy dataset of Real‐world Task‐driven Testing Set (RTTS) and the natural image dataset of pattern analysis statistical modelling and computational learning, visual object classes (PASCAL VOC) are conducted. The results show that the proposed model can be applied on the degraded dataset of RTTS but fails on PASCAL VOC.
Design of a non‐ferromagnetic spoke array Vernier permanent magnet generator with iron‐bar based on enhanced equivalent magnetic network model
Authors: Ghods Mehrage , Faiz Jawad , Rashipour Ehsan , Bazrafshan Mohammad Amin , Besmi Mohammad Reza 
Published: December 2022

AbstractThe spoke‐type arrangement of permanent magnets (PMs) in rotor of Vernier PM (VPM) machines was first introduced in a dual‐stator topology to overcome low‐power drawback of VPM machines. However, this structure is mostly applicable for high‐power motor drives. A non‐ferromagnetic spoke array VPM generator with a special rotor structure for small‐scale wind turbine generator is presented. The leakage flux lines tend to be aligned with the main flux routes due to a special design of flux barriers in end portion of PM housings. High torque‐density, low weight, torque‐speed curve with a wide constant torque region, promising efficiency, and power factor are the advantages of the proposed machine. An Equivalent‐Magnetic‐Network (EMN) model is established for predicting the complex behaviour of flux lines and operating quantities of the proposed structure with reasonable accuracy. The contribution of the modelling method includes a special mesh cell for the air‐gap permeance network to more accurately capture the flux routes, continuous positioning method for omitting spikes on post‐processing waveforms due to numerical errors, and a stable solving flowchart with a high convergence rate. The validity of the EMN model is validated by comparing the operating waveforms of the machine with the corresponding waveforms obtained by the finite element and experiments.The cover image is based on the Research Article Design of a non‐ferromagnetic spoke array Vernier permanent magnet generator with iron‐bar based on enhanced equivalent magnetic network model by Mehrage Ghods et al., https://doi.org/10.1049/elp2.12272.