bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single cell sequencing reveals microglia induced angiogenesis by specific subsets of endothelial cells following spinal cord injury
Authors: Yao Chun , Cao Yuqi , Lv Yehua , Wang Dong , Liu Yan , Gu Xiaosong , Wang Yongjun , Wang Xuhua , Yu Bin 
Published: January 2022

AbstractSpinal cord injury (SCI) results in dynamic alterations of the microenvironment at the lesion site, which inevitably leads to neuron degeneration and functional deficits. The prominent deterioration of the milieu, derived from the destruction of spinal vascular system, not only activates innate immunity but also makes cells in the lesion lose nutrient supports. Limited endogenous angiogenesis happens after SCI, but the cell events at the lesion site underpinning this process have not been delineated so far. Here, we conducted single-cell RNA sequencing (scRNA-seq) of tissues in the spinal lesion at different time points after rat SCI. After performing clustering and cell-type identification, we focused on the vascular endothelial cells (ECs), which play a pivot role in angiogenesis, and drew a comprehensive cellular and molecular atlas for endogenous angiogenesis after SCI. We found that microglia and macrophage promote endogenous angiogenesis by regulating EC subsets through SPP1 and IGF1 signal pathways. Our results indicated that immune cells promotes angiogenesis by the regulation of specific cell subsets of vascular ECs, which provides new clues for the development of interventions for SCI.
High throughput RNA sequencing discovers symptomatic and latent viruses: an example from ornamental Hibiscus
Authors: Zhou Shuyu , Richert-Pöggeler Katja R. , Wang Ziwei , Schwarzacher Trude , Heslop-Harrison J.S. (Pat) , Liu Qing 
Published: January 2022

ABSTRACTHibiscus rosa-sinensis L. (Hibiscus, Malvaceae) is an ornamental species grown widely in amenity plantings. We collected leaves on an urban roadside pavement (sidewalk) near a market in Guangzhou which showed multiple symptoms of leaf rolling, deformation and chlorosis. Initial evaluation by electron microscopy using negative staining of drip preparations revealed the presence of tobamovirus-like particles. Total RNA was extracted, and, unusually, without any RNA selection based on sequence, was used for cDNA library construction and high-throughput survey sequencing. From the 814 Mb of clean sequence data (from 2,712,161 paired reads of 150 bp) reads representing chloroplast, ribosomal, and mitochondrial genes were filtered out, eliminating 79.1% of reads. 1,135,848 × 150 bp of the sequence was retained and screened for viral sequences. Assembly of these sequences detected nine virus species from seven virus genera comprising three tobamoviruses, namely, Tobacco mosaic virus, Tobacco mild green mosaic virus and Hibiscus latent Singapore virus, Turnip mosaic virus (Potyvirus), Potato virus M (Carlavirus), Hibiscus chlorotic ringspot virus (Betacarmovirus), Fabavirus sp (Fabavirus), Cotton leaf curl Multan virus (Begomovirus) and a putative mitoviruses replicating in mitochondria, Chenopodium quinoa mitovirus 1. Mapping the reads to complete virus reference sequences showed high and uniform coverage of the genomes from 3,729 x coverage for Turnip mosaic virus to 22 x for Cotton leaf curl Multan virus. By comparison, nuclear reference genes actin showed 14 x coverage and polyubiquitin 27 x. Notable variants from reference sequences (SNPs) were identified. With the low cost of sequencing and potential for semi-automated bioinformatic pipelines, the whole-RNA approach has huge potential for identifying multiple undiagnosed viruses in ornamental plants, resulting in the ability to take preventive measures in production facilities against spread and to product quality for the mutual benefit of producers and consumers.
The curious case of green human resource management practices in the Ghanaian manufacturing industry; a reality or a mirage?
Authors: Suleman Abdul-Razak , Amponsah-Tawiah Kwesi , Adu Isaac Nyarko , Boakye Kwame Owusu 
Published: January 2022

PurposeBased on the legitimacy theory, this study sought to explore specific Green Human Resource Management Practices implemented by manufacturing firms in Ghana.Design/methodology/approachThe study purposively sourced data from five manufacturing firms operating in Ghana, specifically, Cocoa Processing Company, Dannex Aryton Starwin PLC, Fan Milk Limited, Guinness Ghana Breweries Limited and Unilever Ghana PLC. The qualitative data from 10 interviewees were thematically analysed.FindingsPervasively, it was evident that Ghanaian manufacturing firms have integrated environmental concerns into their human resource management functions, hence they place more emphasis on online tools and platforms in attracting, selecting, involving, training, paying and managing the performance of employees. Lastly, manufacturing firms operating in Ghana give priority to online job applications ahead of manual applications.Practical implicationsIn an era of sustainability, green human resource management practices provide a basis for the legitimation and sustenance of businesses through enhanced environmental performance and its resultant corporate image.Originality/valueThe study highlights the extent to which green human resource management is practised in a Sub-Saharan African country and the specific practices adopted in promoting the concept.
Pharmacological interventions to diminish cognitive side effects of electroconvulsive therapy: A systematic review and meta‐analysis
Authors: Verdijk Joey P.A.J. , van Kessel Mike A. , Oud Matthijs , Kellner Charles H. , Hofmeijer Jeannette , Verwijk Esmée , van Waarde Jeroen A. 
Published: January 2022

AbstractObjectiveThe authors conducted a systematic review and meta‐analysis of pharmacological interventions to diminish cognitive side effects of ECT.MethodsElectronic databases of Pubmed, PsycInfo, Embase and Scopus were searched from inception through 1 April, 2021, using terms for ECT (e.g. electroconvulsive therapy), cognitive outcome (e.g. cogni*) and pharmacological intervention (e.g. calcium channel blocker and general terms, like protein). Original studies with humans receiving ECT were included, which applied pharmacological interventions in comparison with placebo or no additive intervention to diminish cognitive side effects. Data quality was assessed using Risk of Bias and GRADE. Random‐effects models were used. PROSPERO registration number was CRD42021212773.ResultsQualitative synthesis (systematic review) showed 52 studies reporting sixteen pharmacological intervention‐types. Quantitative synthesis (meta‐analysis) included 26 studies (1387 patients) describing twelve pharmacological intervention‐types. Low‐quality evidence of efficacy was established for memantine (large effect size) and liothyronine (medium effect size). Very low‐quality evidence shows effect of acetylcholine inhibitors, piracetam and melatonin in some cognitive domains. Evidence of no efficacy was revealed for ketamine (very low‐quality), herbal preparations with anti‐inflammatory properties (very low to low‐quality) and opioid receptor agonists (low‐quality).ConclusionMemantine and liothyronine are promising for further research and future application. Quality of evidence was low because of differences in ECT techniques, study populations and cognitive measurements. These findings provide a guide for rational choices of potential pharmacological intervention research targets to decrease the burden of cognitive side effects of ECT. Future research should be more uniform in design and attempt to clarify pathophysiological mechanisms of cognitive side effects of ECT.
The quality and readability of online molar incisor hypomineralization patient education materials: a systematic analysis
Authors: Meade MJ , Dreyer CW 
Published: January 2022

AbstractBackgroundMolar incisor hypomineralization (MIH) is estimated to affect 14% of children worldwide. It is crucial that patients and their families have access to easily comprehensible and reliable MIH‐relevant information. This study aims to determine the quality, reliability and readability of online patient education materials about MIH.MethodsA systematic search strategy was adopted. Five validated tools were used to assess the content of the 21 websites that satisfied inclusion/exclusion criteria. Data analyses were applied via GraphPad Prism software version 9 (GraphPad Software, San Diego, CA, USA).ResultsFive (23.8%) websites only satisfied the criteria for understandability and two (9.5%) websites satisfied the criteria for actionability using the Patient Education Materials Assessment Tool (PEMAT). No website contained the Health on the Net (HON)Code Seal and the mean (SD) Journal of the American Medical Association number of benchmarks per website was 1.33/4 (1.02). All websites failed to reach recommended minimum readability levels. Higher PEMAT scores were associated with ‘easier’ readability.ConclusionsOnline patient education materials related to MIH are lacking in quality and reliability, and are too difficult for most to read easily. The authors of MIH‐related online content should consider reference to quality of information tools when developing patient education materials.
Identification of processed pseudogenes in the genome of Thoroughbred horses: Possibility of gene‐doping detection considering the presence of pseudogenes
Authors: Tozaki Teruaki , Ohnuma Aoi , Kikuchi Mio , Ishige Taichiro , Kakoi Hironaga , Hirota Kei‐ichi , Kusano Kanichi , Nagata Shun‐ichi 
Published: January 2022

AbstractProcessed pseudogenes, also known as retrocopy genes, are copies of messenger RNAs that have been reverse transcribed into DNA and inserted into the genome. In this study, we identified 62 processed pseudogene candidates as intron‐less genes from whole‐genome sequencing (WGS) data of Thoroughbred horses using delly structural variation software. The 62 processed pseudogene candidates were confirmed by PCR amplification of intron‐less products. A total of 11 processed pseudogenes were confirmed in the genome of all 23 analysed horses, whereas three processed pseudogenes with structures of ATP11B, DPH3 and RPL17 were detected in only one of 115 horses by PCR amplification of intron‐less products. Currently, most of the gene doping tests proposed in human and horse sports are adapted PCR‐based methods using hydrolysis probes to detect exon/exon junctions in transgenes because the operation is simple and economical. However, when the pseudogene is present in the host genome, the PCR‐based methods may have a potential risk of detecting false positives. In this study, because processed pseudogenes that exist less frequently in the horse genome may affect PCR‐based transgene detection in gene‐doping tests, we propose and demonstrate that PCR amplification and sequencing using primers designed on transgene and promotors and/or polyadenylation signal for gene expression are useful for gene‐doping detection as an additional confirmatory test to prevent false positives.
Association of Apgar score at 5 minutes with academic performance and intelligence in youth: A cohort study
Authors: Ladelund Agnes Kielgast , Bruun Frederik Jager , Slavensky Julie Anna , Ladelund Steen , Kesmodel Ulrik Schiøler 
Published: January 2022

AbstractIntroductionThe Apgar score is routinely given at childbirth worldwide. A low Apgar score at 5 minutes is a strong predictor for neonatal death. Scores below 7 have been associated with higher risks of later neurologic disability. Few studies have assessed the association between Apgar score and school performance and intelligence. The existing literature points towards a possible association between Apgar score and later cognitive function, but the contradictions call for further investigation to fully understand the potential association. This study aimed to examine the possible association between Apgar score at 5 minutes and academic performance and intelligence in youth.Material and methodsThe study is a cohort study. The cohort consists of all Danish liveborn children in 1978–2000 (n = 1 450 681). Data regarding pregnancies, births, parents, school grades, and intelligence of the children were retrieved from different Danish registers. Multiple imputations were performed to avoid discarding data. After exclusion, the final cohort consisted of 1 005 241 children. Associations between Apgar score at 5 minutes and school graduation, grades and attendance, and intelligence scores from conscription were analyzed using univariate and multivariate logistic and linear regressions.ResultsNo association was found between Apgar score and graduating primary school. Adjusted odds ratio (aOR) of graduating upper secondary education and attending conscription were significantly lower for children with scores below 7 compared with 7–10: graduating upper secondary education: Apgar 0–3: aOR 0.79 (95% CI 0.67–0.93), Apgar 4–6: aOR 0.86 (95% CI 0.81–0.93), attending conscription: Apgar 0–3: aOR 0.73 (95% CI 0.59–0.91), Apgar 4–6: aOR 0.73 (95% CI 0.66–0.80). The Apgar 4–6 group had significantly lower total mean primary school grade average: −0.13 (95% CI −0.21 to −0.054) and lower mean intelligence scores at conscription: −0.57 (95% CI −1.09 to −0.058). All other differences remained insignificant.ConclusionsPerformances when graduating school and attending conscription were overall equal regardless of Apgar score at 5 minutes. Chances of graduating primary school were the same irrespective of the score, but chances of graduating upper secondary education and attending conscription were significantly lower with scores below 7. The results suggest that children with scores below 7 may fail to appear at upper secondary education and conscription, but if they do, they perform equally to anyone else.
1013. TANACETUM DENSUM subsp. AMANI
Authors: Hind Nicholas , Strange Kit , King Christabel 
Published: January 2022

SummaryTanacetum densum (Labill.) Sch.Bip. subsp. amani Heywood (Compositae: Anthemideae: Anthemidinae) is illustrated and described. Notes on cultivation, propagation, pests and diseases and availability are provided. The position of Tanacetum in the tribe Anthemideae is discussed, as well as the size of the genus, and previous treatment of many of the species in Pyrethrum. The homonymy of Pyrethrum Zinn is outlined, and the likely position of P. bidens Medik. is discussed: a position in either Acmella or Spilanthes, in the tribe Heliantheae, is proposed. The phytochemistry of Tanacetum densum subsp. amani is discussed.
On the influence of environmental factors on the oviposition activity of necrophagous flies
Authors: Lutz Lena , Verhoff Marcel A. , Rosenbaum Tabea , Amendt Jens 
Published: January 2022

Abstract1. Locating an optimal oviposition site can be a challenging task for female insects, especially when dealing with a patchy, unpredictable and ephemeral food source such as carrion. Understanding the biotic and abiotic parameters that influence the oviposition behaviour of necrophagous flies is not just of great biological importance but also essential for their application in legal investigations.2. In this study, we monitored the oviposition activity of necrophagous flies (Calliphoridae, Sarcophagidae) using mouse carcasses in an urban (city) and a rural (mixed forest) habitat in Frankfurt/Germany over a 2‐year period.3. Over 240 sampling days, 220,963 larvae of 4 blow fly species and 1 flesh fly were sampled. The most abundant species was the blow fly Lucilia ampullacea, followed by its family members Calliphora vicina and Lucilia caesar, the flesh fly Sarcophaga caerulescens and Lucilia sericata. Up to seven environmental parameters were statistically significant predictors for a colonisation of the carcasses, leading to unique patterns of seasonal and daily oviposition activity for all five species.4. Overall, the analysis showed that the seasonal adaption (the phenology of each species), the habitat (rural vs. urban) as well as temperature are the most important factors influencing the oviposition behaviour and activity of necrophagous blow flies and flesh flies.
Referentiality mechanisms in EU education policymaking: The case of the European Education Area
Authors: Kushnir Iryna 
Published: January 2022

AbstractIn 2017, the European Union (EU) initiated the development of the European Education Area (EEA). The referentiality approach frames an original and timely inquiry into how EU policymakers explain the reasons for the development of the EEA. This inquiry relies on thematic analysis of relevant key policy materials, produced by the European Commission and the Council of the EU. The findings highlight a range of internal and external referentiality mechanisms that EU policymakers use to justify the development of the EEA. Internal references are related mainly to education traditions in the EU as a strong foundation for the EEA and the source of its ideas; the beliefs that the EEA can help overcome the interlocking crises the EU has faced in its recent past in the area of education as well as more generally; as well as the organisation of the EEA as part of the functioning of the EU, with both mutually shaping each other. External references are used to build on this and boost the competitiveness of EU's education sector and the EU as a region in the world. These findings address important gaps in our knowledge about EU policymakers' reasons for the development of the EEA. They enrich the limited literature about the EEA, make a theoretical contribution by extending the application of the referentiality approach and call policy actors at all levels of the EEA to facilitate a shared understanding of the reasons for the development of the EEA to ensure that their work serves EEA's purpose.
Value cocreation in livestreaming and its effect on consumer‐simulated experience and continued use intention
Authors: Chou Cindy Yunhsin , Chen Ja‐Shen , Lin Shao‐Kai 
Published: January 2022

AbstractDrawing on the literature related to value cocreation and narrative transportation, this study proposes a framework for examining the relationships among consumer and livestreamer value cocreation behaviors, consumers’ mentally simulated experiences, and their intention to continue using livestreaming services. Purposive and snowball sampling were used to recruit survey participants. A total of 463 individuals were enrolled to test the research model and its hypotheses. The value cocreation of consumers and livestreamers affects consumers’ mentally simulated experiences, which in turn affect their intention to continue using a livestreaming service. To verify this finding, a post hoc in‐depth interview was conducted with an e‐commerce manager with extensive experience in producing livestreaming programs. This study differs from previous works in this area in that it focuses on what drives consumers to use livestreaming services and examines the effects of value cocreation on consumers’ mentally simulated experiences, narrative transportation, and their intention to continue using livestreaming services.
Late gadolinium enhancement cardiac magnetic resonance imaging of ablation lesions after postinfarction ventricular tachycardia ablation: Implications for ventricular tachycardia recurrence
Authors: Ghannam Michael , Liang Jackson , Attili Anil , Cochet Hubert , Jais Pierre , Latchamsetty Rakesh , Jongnarangsin Krit , Morady Fred , Bogun Frank 
Published: January 2022

AbstractBackgroundLate gadolinium enhancement cardiac magnetic resonance (LGE‐CMR) imaging distinguishes between intrinsic postinfarction scar and radiofrequency ablation lesion related scar (dark core lesions [DCLs]) in patients with prior ventricular tachycardia (VT) ablation procedures.ObjectiveTo combine LGE‐CMR and electroanatomic mapping data to describe the relationship between DCLs and recurrent VT among patients undergoing repeat ablations for postinfarction VT.MethodsConsecutive patients with repeat ablation for postinfarct VT with LGE‐CMR before the repeat procedures were studied. Prior ablation procedures and implantable cardiac defibrillator electrograms were analyzed to determine new versus previously documented VT. DCLs were identified on preprocedure LGE‐CMR and registered to electroanatomic maps. A control group of patients undergoing repeat ablation procedures without imaging was included.ResultsNineteen study patients and 14 control patients were followed for 2.6 (1.6–5.6) years (31 [94%] men, age 65.8 ± 8.4 years, ejection fraction 24.7 ± 10.3, p > 0.10 for all). DCLs corresponded to unexcitable tissue during repeat procedures (area 22.4 ± 15.1 vs. 22.9 ± 16.8 cm3, correlation coefficient = .93). Most VT target sites (39/50 [78%]) were in close proximity (<1 cm) to DCLs. Most DCL related VTs 32/39 (82%) were new VTs. Patients with LGE‐CMR imaging incorporated into their ablation procedures had improved 24‐month survival from VT (64% vs. 38%, log rank p < 0.02).ConclusionLGE‐MRI can identify prior ablation lesions corresponding to nonexcitable tissue during repeat ablation procedures for postinfarction VT. VT target sites are often located in close proximity to the DCL area that may function as a fixed border for reentry circuits. Registration of DCL from prior ablation may facilitate repeat ablation procedures.
Relationship between α‐genus human papillomavirus and non‐genital seborrheic keratosis: Report of new cases and updated review
Authors: Nellessen Tobias , Aoki Rui , Kammerbauer Claudia , Clanner‐Engelshofen Benjamin M. , French Lars E. , Reinholz Markus 
Published: January 2022

AbstractBackgroundSeborrheic keratoses (SK) are the most common acquired benign tumor that affects middle‐aged or older adults with great cosmetic concern. Clinical and histopathological similarities of SK and common warts have been addressed by investigating the possible presence of human papillomavirus (HPV) DNA in SK. Previous studies suggested the association between α‐genus HPV and SK located on genital skin, whereas the causal relationship between α‐HPV and non‐genital SK remains controversial.AimThis study aimed to clarify the pathogenic involvement of α‐HPV in the development of non‐genital SK.MethodsWe analyzed α‐HPV DNA prevalence and HPV genotypes using a PCR‐based microarray on 51 skin samples presenting with histologically confirmed SK without any malignant changes. Correlation between the histological subtype of SK and their HPV DNA‐positive reactivity was also evaluated.ResultsOf 51 non‐genital SK, two (3.9%) skin samples were positive for α‐HPV DNA; high‐risk HPV 31 and low‐risk HPV 42 were found. Evaluation of HPV prevalence in different histological types of SK showed that both HPV‐positive cases were acanthotic type; 14.3% of acanthotic SK lesions were positive, while all of the other types were negative for α‐HPV.ConclusionsThis study demonstrates that α‐HPV positivity is very rare in common non‐genital SK. The rare α‐HPV‐positive SK lesions histologically belonged to the acanthotic type, implying a potential impact of HPV infection on epidermal hyperproliferation. Although a possible association cannot be excluded, our findings suggest that α‐HPV is not a major causative factor for non‐genital SK.
The rising interconnectedness of the insurance sector
Authors: Jourde Tristan 
Published: January 2022

AbstractThis paper examines the long‐term evolution of the linkages of the insurance sector with financial and nonfinancial companies. We develop a measure of connectedness using a multifactor model of weekly equity returns. The empirical analysis is conducted from 1973 to 2018, for 16 developed countries, at both the sectoral and institution levels. The results indicate that, unlike other sectors, the connectedness level of the insurance industry has strengthened over time. We also find that the linkages of the largest insurance companies with financial and nonfinancial firms are structurally different but as high as those of the largest banks.