bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digestibilidad de nutrientes, bioquímica sanguínea y desempeño de pollos de engorde alimentados con tres fuentes de aceites esenciales
Authors: Márquez Tobar Paul Andrés 
Published: November 2022

Introducción. En la actualidad se están buscando alternativas a la utilización de antibióticos promotores por sus efectos negativos en la salud, una alternativa es la combinación de aceites esenciales. Objetivo. Evaluar los índices de digestibilidad, bioquímica sanguínea y los indicadores productivos en pollos Broiler alimentados con 3 fuentes de aceite esenciales. Metodología. En la primera parte del experimento, 1400 pollos Cobb 500 machos fueron distribuidos en cinco tratamientos (CP, CN, ACT, XRT, PHY), se midió el desempeño productivo y bioquímica sanguínea de aves criadas en jaulas de piso de cemento con cama de cascarilla de arroz reutilizados de dos crianzas continuas sin desinfectar, en la segunda parte del experimento se midió la digestibilidad de nutrientes a los 21 días de edad por el método de colección. Se utilizaron 240 aves seleccionadas de la crianza en piso, fueron alojadas en jaulas metabólicas y distribuidas en los cinco tratamientos, con seis repeticiones y ocho aves por repetición. Resultados. No existieron diferencias significativas en los valores de digestibilidad de proteína cruda, ceniza, materia seca, materia orgánica entre todos los tratamientos (P>0,05), solo se encontraron diferencias en las variables fibra, grasa y extracto libre de nitrógeno (P<0,05), no existieron diferencias significativas en los valores de bioquímica sanguínea a los 21 y 42 días (Colesterol, Triglicéridos, Ácido Úrico, Urea, Creatinina, Proteína Total, Albumina, Globulina) entre todos los tratamientos (P>0,05), no existieron diferencias significativas en la ganancia de peso de la 5-6 semana, el consumo de alimento de la 1-6 semana, en la conversión alimenticia de 1-6 semana (P>0,05) solo se encontraron diferencias significativas en la ganancia de peso de la 1-2 semana y 3-4 semana (P<0,05). Conclusión. La aplicación de aceites esenciales no afecto los valores de bioquímica sanguínea de (Colesterol, Triglicéridos, Ácido Úrico, Urea, Creatinina, Proteína Total, Albumina, Globulina) entre todos los tratamientos.
Clinical significance of the series of CYP2C9*non3 variants, an unignorable predictor of warfarin sensitivity in Chinese population
Authors: Wang Dongxu , Wu Hualan , Dong Min , Zhang Qing , Zhao Anxu , Zhao Xinlong , Chong Jia , Du Minghui , Wang Yan , Shi Haifeng , Wang Shuanghu , Wang Fang , Cai Jianping , Yang Jiefu , Dai Dapeng , Chen Hao 
Published: November 2022

BackgroundsGene polymorphisms are critical for variations in warfarin dose. To date, more than 70 CYP2C9 alleles have been identified. This study was designed to clarify the clinical significance of CYP2C9*non-3 variants to warfarin sensitivity in Chinese Han patients.MethodsThe entire CYP2C9 gene region was sequenced in 1,993 individuals, and clinical data and VKORC1 genotypes were collected from 986 patients with atrial fibrillation treated with warfarin. The SKAT-O method was used to analyze the effects of CYP2C9*non-3 variants on warfarin sensitivity.ResultsA total of 20 CYP2C9 variants were identified, of which four were novel. Carriers with CYP2C9*non-3 variants may have lower warfarin dose requirements, and similar to CYP2C9*3, CYP2C9*non-3 variants are clearly relevant to warfarin-sensitive and highly sensitive responders.ConclusionOur results showed that, besides CYP2C9*3, the series of CYP2C9*non-3 variants is an unignorable predictor for warfarin sensitivity in Chinese population. From a safety consideration, people carried such variants may need a preferred choice of NOACs when they started anticoagulation therapy.
An unusual contrast-induced encephalopathy following percutaneous coronary intervention in patients with cerebrovascular abnormalities: A case report
Authors: Liao Jiangquan , Wang Yan , Shao Mingjing , Wang Yanling , Du Jinhang , Li Xianlun , Yang Peng , Fu Dongliang , Dong Zhe , Liu Mengru 
Published: November 2022

IntroductionContrast-induced encephalopathy (CIE) is a complication associated with the administration of iodinated contrast, which usually happens minutes to hours after contact with contrast, and fully recovers within 72 h. The clinical manifestations of CIE are diverse, and the pathological mechanism is not explicit.MethodsWe report the case of a 66-year-old female who suffered from a delayed CIE following the administration of iodinated contrast agent. Symptoms were severe. Imaging examination, biochemical and etiological detection were performed timely. The course of neurological symptoms was atypical. Her complex complications of hypothyroidism and cerebrovascular abnormalities contributed to more challenges, which were also clues to the diagnosis. With prompt and active treatment, the patient recovered fully over 10 days.DiscussionThe diagnosis standard of CIE highly depends on the association with the contact of contrast and the exclusion of other nervous system diseases. Complicated clinical circumstances and individual specificity can lead to different clinical manifestations of CIE, making it even more difficult to diagnose and treat. Prompt and dynamic imaging examination would provide great value in the diagnosis and evaluation of CIE. Timely diagnosis and intervention may be the key to its satisfying prognosis.
Which one? A suggested approach for evaluating digital health maturity models
Authors: Woods Leanna , Eden Rebekah , Duncan Rhona , Kodiyattu Zack , Macklin Sophie , Sullivan Clair 
Published: November 2022

BackgroundDigital health maturity models allow healthcare organizations to evaluate digital health capability and to develop roadmaps for improving patient care through technology. There are many models available commercially for healthcare providers to use to assess their digital health maturity. Currently, there are limited evidence-based methods to assess the quality, utility, and efficacy of maturity models to select the most appropriate model for the given context.ObjectiveTo develop a framework to assess digital maturity models and facilitate recommendations for digital maturity model selection.MethodsA systematic, consultative, and iterative process was used. Literature analyses and a stakeholder needs analysis (n = 23) was conducted to develop content and design considerations. These considerations were incorporated into the initial version of the framework developed by researchers in a design workshop. External stakeholder review (n = 20) and improvements strengthened and finalized the framework.ResultsThe criteria of the framework include assessment of healthcare context, feasibility, integrity, completeness and actionability. Users can compare model performance in order to select the most appropriate model for their context.ConclusionThe framework provides healthcare stakeholders with a consistent and objective methodology to compare digital health maturity models, informing approaches to choosing a suitable model. This is a critical step as healthcare evolves towards a digital health system focused on improving the quality of care, reducing costs and improving the provider and consumer experience.
Blood glucose may be another index to initiate insulin treatment besides glycated hemoglobin A1c after oral antidiabetic medications failure for glycemic control: A real–world survey
Authors: Li Yanli , Wu Yan , Shu Yi , Li Shu , Pei Jianhao , Chen Hong , Liu Shiping , Xiang Guangda , Wang Wenbo , Shan Pengfei , Su Heng , Wu Xiaoyan , Yan Dewen , Li Wangen 
Published: November 2022

ObjectiveThe inertia of insulin initiation is a barrier to achieving glycemic control when oral antidiabetic drugs fail to control glucose during the treatment of type 2 diabetes (T2D). Insulin initiation is usually based on glycated hemoglobin A1c (A1C). To investigate whether there is another index for insulin initiation besides A1C, we conducted a cross-sectional survey in the real world.MethodsWe conducted a multicenter cross-section survey with a total of 1034 T2D patients. All patients, at the time of the survey, decided to initiate insulin therapy due to failure of controlling glucose using only oral antidiabetic drugs. We analyzed the differences of blood glucose between patients who were tested for A1C and those who were not.Results666 (64.4%) patients were tested A1C and 368 (35.6%) were not. Neither fasting blood glucose (FBG) (12.0 ± 2.9 vs 12.3 ± 2.9 mmol/L, t = 1.494, P = 0.135) nor postprandial blood glucose (PBG) (18.4 ± 4.8 vs 17.9 ± 4.8 mmol/L, t = 1.315, P = 0.189) were significantly different between patients with and without A1C.ConclusionOur results demonstrated that initiating insulin based on FBG or PBG is a common clinical practice, at least in China; moreover, since it is easier to obtain than A1C, it can be a simple and effective way to overcome clinical inertia for initiating insulin.
Food preferences in a generalist pollen feeder: A nutritional strategy mainly driven by plant carbohydrates
Authors: Bellec Laura , Cortesero Anne-Marie , Giguère Thomas , Faure Sébastien , Hervé Maxime R. 
Published: November 2022

IntroductionAnimal nutritional strategies have been extensively studied in vertebrates, where generalism at the individual scale is the rule. In insect herbivores, the determinants of the nutritional strategy of individual-scale generalists remain poorly studied, and the focus has been placed mainly on the influence of plant defense. Moreover, the integration of a physiological dimension in such studies remains rare. Here, we investigated the determinants of the nutritional strategy of pre-diapausing pollen beetles,Brassicogethes aeneus, with a focus on the influence of macronutrients. Before their diapause, pollen beetles are known to feed from plants belonging to many different families. This raises three questions: (i) Is the generalism of pollen beetles a populational consequence of individuals specialized on different plant families? (ii) Do individuals feed at random on flowers available or do they have a particular nutritional strategy? and (iii) In case of non-random feeding choices, do pollen macronutrients explain this nutritional strategy?MethodsTo answer these questions, we used a series of laboratory experiments including feeding choice tests on flowers and artificial substrates, quantification of pollen nutrient content, quantification of the insect energetic budget, and performance experiments.ResultsWe show that pollen beetles are generalist at the individual scale, and that clear and stable food preferences are established over a few hours in a multi-choice context. Pollen beetles prefer to feed on flowers with a carbohydrate-rich pollen, and this preference is adaptive since performance correlates positively with the plant carbohydrate content. This better performance may be explained by the fact that individuals feeding on carbohydrate-rich resources accumulate more glycogen and total energetic reserves.DiscussionThis study represents one of the few evidences of generalism at the individual scale in an herbivorous insect. It provides a better understanding of the nutritional strategy of a non-bee pollen feeder and shows the importance of carbohydrates in this strategy. It highlights the need to combine assessments of the plant macronutrient content and insect energetic budget in an adaptive framework to better understand the nutritional strategies of herbivores.
Manipulation of diacylglycerol and ERK-mediated signaling differentially controls CD8+ T cell responses during chronic viral infection
Authors: Harabuchi Shohei , Khan Omar , Bassiri Hamid , Yoshida Taku , Okada Yohei , Takizawa Masaomi , Ikeda Osamu , Katada Akihiro , Kambayashi Taku 
Published: November 2022

IntroductionActivation of T cell receptor (TCR) signaling is critical for clonal expansion of CD8+ T cells. However, the effects of augmenting TCR signaling during chronic antigen exposure is less understood. Here, we investigated the role of diacylglycerol (DAG)-mediated signaling downstream of the TCR during chronic lymphocytic choriomeningitis virus clone 13 (LCMV CL13) infection by blocking DAG kinase zeta (DGKζ), a negative regulator of DAG.MethodsWe examined the activation, survival, expansion, and phenotype of virus-specific T cell in the acute and chronic phases of LCMV CL13-infected in mice after DGKζ blockade or selective activation of ERK.ResultsUpon LCMV CL13 infection, DGKζ deficiency promoted early short-lived effector cell (SLEC) differentiation of LCMV-specific CD8+ T cells, but this was followed by abrupt cell death. Short-term inhibition of DGKζ with ASP1570, a DGKζ-selective pharmacological inhibitor, augmented CD8+ T cell activation without causing cell death, which reduced virus titers both in the acute and chronic phases of LCMV CL13 infection. Unexpectedly, the selective enhancement of ERK, one key signaling pathway downstream of DAG, lowered viral titers and promoted expansion, survival, and a memory phenotype of LCMV-specific CD8+ T cells in the acute phase with fewer exhausted T cells in the chronic phase. The difference seen between DGKζ deficiency and selective ERK enhancement could be potentially explained by the activation of the AKT/mTOR pathway by DGKζ deficiency, since the mTOR inhibitor rapamycin rescued the abrupt cell death seen in virus-specific DGKζ KO CD8+ T cells.DiscussionThus, while ERK is downstream of DAG signaling, the two pathways lead to distinct outcomes in the context of chronic CD8+ T cell activation, whereby DAG promotes SLEC differentiation and ERK promotes a memory phenotype.
Plasma adsorption in refractory chronic gouty arthritis flare: A case report
Authors: Lei Yan , Cui Tian-Jiao , Wang Xiao-Hua , Zhang Xue-Mei , Tang Chun , Zheng Zhi-Hua 
Published: November 2022

BackgroundAlong with uric acid, which is the primary driving factor of gout, downstream inflammatory mediators have been shown to be involved in the pathogenesis of gouty arthritis flares. Extracorporeal haemadsorption is an emerging technology for the treatment of dysregulated inflammatory states by effectively removing cytokines from the bloodstream. Whether haemadsorption was effective in refractory gout flares has not been reported in the literature.Case summaryWe report the case of a 52-year-old male who presented with refractory gouty arthropathy for 30 years. His uric acid levels were poorly controlled due to poor diet and treatment compliance. Tophi were found to have precipitated in multiple joints and subcutaneous tissue. In the last 2 years, his incidents of gouty flares had become more frequent, and resistant to the medications, including colchicine, allopurinol, febuxostat, glucocorticoids, and NSAID analgesics. He had experienced a triad of chills, high fever and arthritis for the past 2 weeks. Therefore, he took 2 mg colchicine twice daily for 2 weeks with no improvement in his pain. Proinflammatory cytokines, such as interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α), were found to be remarkably elevated. Given that conventional treatment was unsuccessful, we tried to employ plasma adsorption (PA) to remove inflammatory cytokines. After 4 sessions, symptoms, such as fever, joint swelling and pain, were greatly improved. Meanwhile, the levels of proinflammatory factors such as IL-6 and TNF-α were found to be decreased, while the anti-inflammatory factor IL-10 remained the same during the course. He was followed up for 8 months and arthritis have flared up twice in response to a high-purine diet.ConclusionOur study suggests that plasma adsorption (PA) may be a promising and feasible treatment for refractory gout when conventional treatments are unsatisfactory or contraindicated. However, more clinical trials are needed to verify the efficacy and safety of the treatment.Core tipChronic gouty arthritis flares are refractory to conventional treatment, such as uric acid-lowering drugs and NSAID analgesics. Due to the involvement of inflammatory cytokines, plasma adsorption was employed to alleviate flares by removing inflammatory mediators. Herein, we report a 52-year-old male who presented with refractory gouty arthropathy for 30 years, manifested with a triad of chills, high fever and arthritis. He underwent several sessions of plasma adsorption, and his symptoms soon improved, along with a drop in inflammatory mediators. We conclude that plasma adsorption may be a promising and feasible treatment for refractory gout when conventional treatments are unsatisfactory or contraindicated.
LATPS, a novel prognostic signature based on tumor microenvironment of lung adenocarcinoma to better predict survival and immunotherapy response
Authors: Huang Jihong , Yuan Lu , Huang Wenqi , Liao Liwei , Zhu Xiaodi , Wang Xiaoqing , Li Jiaxin , Liang Wenyu , Wu Yuting , Liu Xiaocheng , Yu Dong , Zheng Yunna , Guan Jian , Zhan Yongzhong , Liu Laiyu 
Published: November 2022

BackgroundClinically, only a minority of patients benefit from immunotherapy and few efficient biomarkers have been identified to distinguish patients who would respond to immunotherapy. The tumor microenvironment (TME) is reported to contribute to immunotherapy response, but details remain unknown. We aimed to construct a prognostic model based on the TME of lung adenocarcinoma (LUAD) to predict the prognosis and immunotherapy efficacy.MethodsWe integrated computational algorithms to describe the immune infiltrative landscape of LUAD patients. With the least absolute shrinkage and selection operator (LASSO) and Cox regression analyses, we developed a LUAD tumor microenvironment prognostic signature (LATPS). Subsequently, the immune characteristics and the benefit of immunotherapy in LATPS-defined subgroups were analyzed. RNA sequencing of tumor samples from 28 lung cancer patients treated with anti-PD-1 therapy was conducted to verify the predictive value of the LATPS.ResultsWe constructed the LATPS grounded on four genes, including UBE2T, KRT6A, IRX2, and CD3D. The LATPS-low subgroup had a better overall survival (OS) and tended to have a hot immune phenotype, which was characterized by an elevated abundance of immune cell infiltration and increased activity of immune-related pathways. Additionally, tumor immune dysfunction and exclusion (TIDE) score was markedly decreased in the LATPS-low subgroup, indicating an enhanced opportunity to benefit from immunotherapy. Survival analysis in 28 advanced lung cancer patients treated with an anti-PD-1 regimen at Nanfang hospital revealed that the LATPS-low subgroup had better immunotherapy benefit.ConclusionLATPS is an effective predictor to distinguish survival, immune characteristics, and immunotherapy benefit in LUAD patients.
Candida albicans V132 induces trained immunity and enhances the responses triggered by the polybacterial vaccine MV140 for genitourinary tract infections
Authors: Martín-Cruz Leticia , Angelina Alba , Baydemir Ilayda , Bulut Özlem , Subiza José Luis , Netea Mihai G. , Domínguez-Andrés Jorge , Palomares Oscar 
Published: November 2022

IntroductionRecurrent urinary tract infections (RUTIs) and recurrent vulvovaginal candidiasis (RVVCs) represent major healthcare problems all over the world. Antibiotics and antifungals are widely used for such infectious diseases, which is linked with microbial resistances and microbiota deleterious effects. The development of novel approaches for genitourinary tract infections (GUTIs) such as trained immunity-based vaccines (TIbV) is therefore highly required. MV140 is a sublingual whole-cell heat-inactivated polybacterial preparation with demonstrated clinical efficacy for RUTIs. The sublingual heat-inactivated Candida albicans vaccine V132 has been developed for RVVCs. We previously showed that the combination of MV140 and V132 promotes potent Th1/Th17 and regulatory T-cell responses against antigens contained in the formulation and unrelated antigens. The specific contribution of each preparation to such effects and the underlying molecular mechanisms remain incompletely understood.MethodsPBMC and monocytes were isolated from healthy donors and in vitro stimulated with V132, MV140 or MV140/V132. After 6 days of resting, cells were reestimulated with LPS and MV140. Analysis of cytokine production by ELISA, Seahorse assays for functional metabolic experiments and chromatin immunoprecipitation assays were performed. BALB/c mice were intraperitoneally and sublingually immunized with V132.ResultsWe uncover that V132 induces trained immunity in human PBMCs and purified monocytes, significantly increasing the responses triggered by subsequent stimulation with MV140. Mechanistically, V132 drives metabolic rewiring towards increased glycolysis and oxidative phosphorylation and induces epigenetic reprogramming that enhances the transcription of the pro-inflammatory genes IL6 and TNFA. Splenocytes and peritoneal cells from V132-immunize mice show increased responses upon in vitro stimulation with MV140. Remarkably, splenocytes from sublingually V132-immunized and MV140 in vivo treatment mice show stronger Th17 responses than mice exposed to excipients upon in vitro stimulation with MV140.ConclusionOverall, we provide novel mechanistic insights into how V132-induced trained immunity enhances both innate and adaptive immune responses triggered by MV140, which might open the door for new interventions for GUTIs with important clinical implications.
Platelet-rich plasma in alopecia areata and primary cicatricial alopecias: A systematic review
Authors: Tejapira Kasama , Yongpisarn Tanat , Sakpuwadol Nawara , Suchonwanit Poonkiat 
Published: November 2022

BackgroundImmune-mediated alopecias (IMAs), a group of hair disorders associated with immunological reactions, remain a therapeutic challenge since available treatments are generally unfavorable with potential side effects. Platelet-rich plasma (PRP) has been recently proposed as a treatment option based on several limited-quality studies; however, there is no systematic evaluation of PRP efficacy on IMAs in the literature.ObjectiveTo assess PRP’s effects in treating IMAs using a systematic review.MethodsElectronic searches were conducted using PubMed, Embase, Scopus, and Cochrane Library databases. A search strategy was designed to retrieve all studies exploring PRP in treating IMAs, including alopecia areata (AA) and primary cicatricial alopecias (PCAs). In addition, all randomized and non-randomized studies reporting subjective and/or objective outcomes of alopecia treatment with PRP were included.ResultsThirty-two studies were included, comprising 621 patients with AA and 19 patients with PCAs. PRP had superior efficacy as monotherapy in five studies, comparable to intralesional corticosteroids in six studies in AA treatment. In addition, in the analysis of PCAs, including lymphocytic and neutrophilic subtypes, PRP was efficacious in alleviating disease progression in nine studies.ConclusionPRP is considered a promising treatment for AA and PCAs in patients who experienced unfavorable outcomes from conventional treatment. However, its clinical application remains to be standardized, and its recommendation as a treatment for IMAs could not be ascertained due to a lack of high-quality evidence.Systematic review registration[https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=353859], identifier [CRD42022353859].