bluuebunny/crossref_metadata_2025_split huggingface dataset:
Methods of choosing a set of means for com-pacting the soils of the sinuses of ditches and trenches on a technical basis
Authors: Basarab Vladimir , Umanets Irina , Sausheva Lyudmila 
Published: July 2022

It is known that soils in a limited space are compacted by specialized means: hand rammers, small equipment (vibrating plates, remote control minikots), and attachments for excavators (vibrating rammers, vibrating plates, inertial rammers, ramming nozzles, etc. guides).
High-quality soil compaction also involves avoiding damage to utilities, building structures, protective and insulating layers on structures and individual structures. It is established that for each compactor based on its power and method of control there is a limit of a certain distance where the soil is compacted with care and restriction, which is called the protective layer of soil.Expert assessment of existing practices of soil compaction during backfilling in the sinuses of ditches shows the difficulty of forming the optimal set of sealing means due to the large number of circumstances related to soil characteristics, geometry of the earth structure, technical characteristics of the means.In this regard, the method of forming such a set is developed, which consists of two stages: the first - the choice of compaction tools on technical grounds, the second - the formation of the optimal set of soil compaction tools from the first stage on economic grounds. This paper considers the first stage of the methodology, which in turn requires a consistent solution of a number of problems.The first - the design of the earth structure on the principle of laying the minimum required geometric parameters or preparation of initial data on the existing project. The second - the formation of an array of data on the principle of increasing the width of the working body of the tool based on the analysis of the existing fleet of soil compaction tools.Third - based on the principle of rational use of resources, limit the number of tools in the set to 3, and divide the vertical cross section of the sinus of the pit into three typical zones. Fourth - automated selection of soil compaction based on the principle of comparing the width of the working body with the width of the typical zone and taking into account the protective layer of soil.
PENGARUH UKURAN PERUSAHAAN KLIEN, AUDIT TENURE, DAN SPESIALISASI AUDITOR TERHADAP KUALITAS AUDIT
Authors: Buchori Ahmad , Budiantoro Harry 
Published: July 2022

Tujuan penelitian ini untuk menemukan bukti empiris mengenai faktor-faktor yang mempengaruhi kualitas audit pada perusahaan di Indonesia. Populasi dalam penelitian ini adalah 148 perusahaan manufaktur berasal dari industri manufaktur yang terdaftar di Bursa Efek Indonesia (BEI) tahun 2011-2015. Metode penentuan sampel pada penelitian ini menggunakan purposive sampling, total sampel adalah 40 perusahaan. Metode analisis data pada penelitian ini menggunakan regresi linear berganda. Hasil penelitian ini menunjukkan ukuran perusahaan berpengaruh positif terhadap kualitas audit, audit tenure berpengaruh negatif terhadap kualitas audit, dan spesialisasi berpengaruh positif terhadap kualitas audit. Secara bersama–sama ukuran perusahaan, audit tenure, dan spesialisasi auditor berpengaruh terhadap kualitas audit.
PENGARUH PIUTANG MURABAHAH, PEMBIAYAAN MUDHARABAH DAN PEMBIAYAAN MUSYARAKAH TERHADAP PROFITABILITAS BANK SYARIAH
Authors: Rianti Febby Angga , Elmanizar Elmanizar 
Published: July 2022

Penelitian ini bertujuan untuk menganalisis pengaruh piutang murabahah, pembiayaan mudharabah dan pembiayaan musyarakah terhadap profitabilitas yang diproksikan dengan return on assets (ROA), return on equity (ROE), rasio biaya operasional (RBO) dan net profit margin (NPM) pada Bank Syariah yang terdaftar di Bank Indonesia periode 2011 – 2015. Metode analisis yang digunakan adalah regresi berganda dengan tingkat signifikan 5% dan deskriptif komparasi dengan rasio non performing financing (NPF) sebagai pembanding. Berdasarkan hasil penelitian piutang murabahah berpengaruh positif dan signifikan terhadap profitabilitas. Pembiayaan mudharabah dan pembiayaan musyarakah berpengaruh secara signifikan terhadap profitabilitas.
Profil Penggunaan Antibiotik Pada Pasien Anak di Rumah Sakit Islam Jakarta Cempaka Putih Selama Periode 1 Januari-31 Desember 2016
Authors: Muti’a Siti , Tola Sakura Muhammad 
Published: July 2022

Latar Belakang: Penggunaan antibiotik yang tidak tepat dapat menimbulkan resistensi bakteri terhadap antibiotik. Terdapat pemberian antibiotik dengan dosis yang tidak sesuai dengan indikasi, usia pasien, berat badan, dan aturan pemberian obat yang benar pada pasien anak yang dirawat di rumah sakit. Tujuan penelitian ini untuk mengetahui profil penggunaan antibiotik (indikasi pemberian, jenis antibiotik, variasi peresepan, rute pemberian dan lamanya terapi), pola uji sensitivitas kuman terhadap antibiotik yang digunakan pada pasien anak di Rumah Sakit Islam Jakarta Cempaka Putih selama periode 1 Januari 2016–31 Desember 2016.Metode: Penelitian ini menggunakan survei deskriptif observasional dengan metode cross sectional. Pengambilan data secara retrospektif dari data sekunder rekam medis.Hasil Penelitian: Pada 100 pasien yang memenuhi kriteria inklusi, terdapat 144 peresepan antibiotik. Diagnosis infeksi terbanyak adalah gastroenteritis(23,0%), demam tifoid (15,0%), bronchopneumonia (12,0%) dan ISPA (10,0%). Antibiotik yang paling banyak digunakan adalah ceftriaxone(49,3%), cefixime(29,2%) dan cefotaxime(9,0%). Variasi peresepan, antibiotik, 1 jenis (60,0%), 2 jenis(36,0%) dan 3 jenis(4,0%). Rute pemberian antibiotik parenteral (68.7%) dan enteral(31,3%). Lama terapi 1–3 hari (93,0%) sedangkan 4–7 hari(8,0%). Seluruh bakteri dari 13 hasil kultur positif, resisten terhadap amoxicillin(92%), resistensi terhadap ceftriaxone(73%), sedangkan sensitivitas meropenem masih tinggi (92%).Kesimpulan: Diagnosis terbanyak gastroenteritis, demam tifoid, bronchopneumonia dan ISPA. Ceftriaxone, cefixime dan cefotaxime adalah antibiotik yang paling banyak digunakan. Amoxicillin resisten pada seluruh bakteri, telah terjadi resistensi pada
Mitophagy-mediated molecular subtypes depict the hallmarks of the tumour metabolism and guide precision chemotherapy in pancreatic adenocarcinoma
Authors: Chen Hao , Zhang Jianlin , Sun Xuehu , Wang Yao , Qian Yeben 
Published: July 2022

Background: Mitophagy is closely related to cancer initiation and progression. However, heterogeneity with reference to mitophagy remains unexplored in pancreatic adenocarcinoma (PAAD).Materials and methods: We used Reactome database to download the mitophagy-related, glycolysis-related and cholesterol biosynthesis-related signaling pathways. Unsupervised clustering using the “ConsensusClusterPlus” R package was performed to identify molecular subtypes related to mitophagy and metabolism. Prognosis-related mitophagy regulators were identified by univariate Cox regression analysis. Receiver operating characteristics (ROC) and Kaplan-Meier (K-M) survival analyses were used to assess the diagnostic and prognostic role of the hub genes and prognosis risk model. Weighted gene co-expression network analysis (WGCNA) was utilized for screening the mitophagy subtype-related hub genes. Metascape was utilized to carry out functional enrichment analysis. The “glmnet” R package was utilised for LASSO, and the “e1071” R package was utilised for SVM. Chemotherapeutic drug sensitivity was estimated using the R package “pRRophetic” and Genomics of Drug Sensitivity in Cancer (GDSC) database. The nomogram was established by the “rms” R package.Results: Three distinct mitophagy subtypes (low, high and intermediate) of PAAD were identified based on the landscape of mitophagy regulators. The high mitophagy subtype had the worst prognosis, highest mRNA expression-based stemness index scores and most hypoxic environment compared to the other subtypes. Additionally, glycolysis and cholesterol biosynthesis were significantly elevated. Three mitophagy subtype-specific gene signatures (CAST, CCDC6, and ERLIN1) were extracted using WGCNA and machine learning. Moreover, PAAD tumours were insensitive to Erlotinib, Sunitinib and Imatinib in the high mitophagy subtype and high CAST, CCDC6, and ERLIN1 expressed subtypes. Furthermore, CAST, CCDC6, and ERLIN1 affected immune cell infiltration (M1 and CD8Tcm), resulting in the altered prognosis of patients with PAAD. A nomogram was constructed to screen patients with the low mitophagy subtype, which showed a higher sensitivity to chemotherapeutic agents.Conclusion: Based on various bioinformatics tools and databases, the PAAD heterogeneity regarding mitophagy was systematically examined. Three different PAAD subtypes having different outcomes, metabolism patterns and chemosensitivity were observed. Moreover, three novel biomarkers that are closely associated with mitophagy and have the potential to guide individualised treatment regimens in PAAD were obtained.
Studying the Inflammatory Responses to Amyloid Beta Oligomers in Brain-Specific Pericyte and Endothelial Co-Culture From Human Stem Cells
Authors: Marzano Mark , Chen Xingchi , Russell Teal A. , Medina Angelica , Wang Zizheng , Hua Timothy , Zeng Changchun , Wang Xueju , Sang Qing-Xiang , Tang Hengli , Yun Yeoheung , Li Yan 
Published: July 2022

Background: Recently, the in vitro blood–brain barrier (BBB) models derived from human pluripotent stem cells have been given extensive attention in therapeutics due to the implications they have with the health of the central nervous system. It is essential to create an accurate BBB model in vitro in order to better understand the properties of the BBB, and how it can respond to inflammatory stimulation and be passed by targeted or non-targeted cell therapeutics, more specifically extracellular vesicles.Methods: Brain-specific pericytes (iPCs) were differentiated from iPSK3 cells using dual SMAD signaling inhibitors and Wnt activation plus fibroblast growth factor 2 (FGF-2). The derived cells were characterized by immunostaining, flow cytometry, and RT-PCR. In parallel, blood vessels organoids were derived using Wnt activation, BMP4, FGF2, VEGF, and SB431542. The organoids were replated and treated with retinoic acid to enhance the blood–brain barrier (BBB) features in the differentiated brain endothelial cells (iECs). Co-culture was performed for iPCs and iECs in the transwell system and 3D microfluidics channels.Results: The derived iPCs expressed common markers PDGFRb and NG2, and brain-specific genes FOXF2, ABCC9, KCNJ8, and ZIC1. The derived iECs expressed common endothelial cell markers CD31, VE-cadherin, and BBB-associated genes BRCP, GLUT-1, PGP, ABCC1, OCLN, and SLC2A1. The co-culture of the two cell types responded to the stimulation of amyloid β42 oligomers by the upregulation of the expression of TNFa, IL6, NFKB, Casp3, SOD2, and TP53. The co-culture also showed the property of trans-endothelial electrical resistance. The proof of concept vascularization strategy was demonstrated in a 3D microfluidics-based device.Conclusion: The derived iPCs and iECs have brain-specific properties, and the co-culture of iPCs and iECs provides an in vitro BBB model that show inflammatory response. This study has significance in establishing micro-physiological systems for neurological disease modeling and drug screening.
High Performance of Commercial PAC on the Simultaneous Desulfurization and Denitrification of Wastewater From a Coal-Fired Heating Plant
Authors: Liu Jiancong , Wang Ying , Wang Yangyang , Wang Zhigang , Wang Xiaoshu , Kong Fanrong , Yan Zheng , Li Tong , Wang Lei 
Published: July 2022

The flue gas desulfurization wastewater is highly saline and has too many refractory pollutants to be recycled during the desulfurization process of the coal-fired heating plant. Given that waste heat is abundant in coal-fired heating plants, a thermal treatment method was developed to simultaneously remove sulfates and nitrates from the wastewater, with the production of chemical-grade natroalunite and recycled water. The results showed that sulfates and nitrates were 50.3 and 10 g/L in the wastewater, respectively, and only 2.8% and 9.1% were removed after direct treatment at 270°C for 7 h; but these rates increased to 99.3% and 99.9%, respectively, with the addition of commercial poly aluminum chloride. Mass balance summarized that the treatment of 1 ton wastewater needed 0.1 ton PAC and produced 0.11 ton natroalunite and 0.92 ton recycle water. The removal of sulfates and nitrates was mainly done by the precipitation reaction of sulfates such as natroalunite and the redox reaction between nitrates and organics, respectively. Thermodynamic analysis demonstrated that the precipitate reaction occurred at 45°C and accelerated in the temperature range of 45–270°C, but became slow with the decrease of sulfate and Al concentrations in wastewater. Four other reagents were also used for wastewater treatment in comparison with PAC and showed the following order of performance: PAC &gt; citrate calcium &gt; limestone &gt; subacetate aluminum &gt; citrate ferric. This method provided a practical route to treat wastewater from flue gas desulfurization without generating secondary waste.
Prevalence and Distribution of Potentially Human Pathogenic Vibrio spp. on German North and Baltic Sea Coasts
Authors: Fleischmann Susanne , Herrig Ilona , Wesp Jessica , Stiedl Joscha , Reifferscheid Georg , Strauch Eckhard , Alter Thomas , Brennholt Nicole 
Published: July 2022

Global ocean warming results in an increase of infectious diseases including an elevated emergence of Vibrio spp. in Northern Europe. The European Centre for Disease Prevention and Control reported annual periods of high to very high risks of infection with Vibrio spp. during summer months along the North Sea and Baltic Sea coasts. Based on those facts, the risk of Vibrio infections associated with recreational bathing in European coastal waters increases. To obtain an overview of the seasonal and spatial distribution of potentially human pathogenic Vibrio spp. at German coasts, this study monitored V. cholerae, V. parahaemolyticus, and V. vulnificus at seven recreational bathing areas from 2017 to 2018, including the heat wave event in summer 2018. The study shows that all three Vibrio species occurred in water and sediment samples at all sampling sites. Temperature was shown to be the main driving factor of Vibrio abundance, whereas Vibrio community composition was mainly modulated by salinity. A species-specific rapid increase was observed at water temperatures above 10°C, reaching the highest detection numbers during the heat wave event with abundances of 4.5 log10 CFU+1/100 ml of seawater and 6.5 log10 CFU+1/100 g of sediment. Due to salinity, the dominant Vibrio species found in North Sea samples was V. parahaemolyticus, whereas V. vulnificus was predominantly detected in Baltic Sea samples. Most detections of V. cholerae were associated with estuarine samples from both seas. Vibrio spp. concentrations in sediments were up to three log higher compared to water samples, indicating that sediments are an important habitat for Vibrio spp. to persist in the environment. Antibiotic resistances were found against beta-lactam antibiotics (ampicillin 31%, cefazolin 36%, and oxacillin and penicillin 100%) and trimethoprim-sulfamethoxazole (45%). Moreover, isolates harboring pathogenicity-associated genes such as trh for V. parahaemolyticus as well as vcg, cap/wcv, and the 16S rRNA-type B variant for V. vulnificus were detected. All sampled V. cholerae isolates were identified as non-toxigenic non-O1/non-O139 serotypes. To sum up, increasing water temperatures at German North Sea and Baltic Sea coasts provoke elevated Vibrio numbers and encourage human recreational water activities, resulting in increased exposure rates. Owing to a moderate Baltic Sea salinity, the risk of V. vulnificus infections is of particular concern.
In Vitro and In Vivo Bactericidal and Antibiofilm Efficacy of Alpha Mangostin Against Staphylococcus aureus Persister Cells
Authors: Felix LewisOscar , Mishra Biswajit , Khader Rajamohammed , Ganesan Narchonai , Mylonakis Eleftherios 
Published: July 2022

The formation of persister cells is associated with recalcitrance and infections. In this study, we examined the antimicrobial property of alpha mangostin, a natural xanthone molecule, against methicillin-resistant Staphylococcus aureus (MRSA) persisters and biofilm. The MIC of alpha mangostin against MRSA persisters was 2 µg/ml, and activity was mediated by causing membrane permeabilization within 30 min of exposure. The membrane activity of alpha mangostin was further studied by fast-killing kinetics of MRSA persiste r cells and found that the compound exhibited 99.99% bactericidal activity within 30 min. Furthermore, alpha mangostin disrupted established MRSA biofilms and inhibited bacterial attachment as biofilm formation. Alpha mangostin down-regulated genes associated with the formation of persister cells and biofilms, such as norA, norB, dnaK, groE, and mepR, ranging from 2 to 4-folds. Alpha mangostin at 16 μg/ml was non-toxic (&gt; 95% cell survival) to liver-derived HepG2 and lung-derived A549 cells, similarly. Still, alpha mangostin exhibited 50% cell lysis of human RBC at 16 μg/ml. Interestingly, alpha mangostin was effective in vivo at increasing the survival up to 75% (p&lt;0.0001) of Galleria mellonella larvae infected with MRSA persister for 120 h. In conclusion, we report that alpha mangostin is active against MRSA persisters and biofilms, and these data further our understanding of the antistaphylococcal activity and toxicity of this natural compound.
Gene expression patterns associated with multidrug therapy in multibacillary leprosy
Authors: Ferreira Helen , Leal-Calvo Thyago , Mendes Mayara Abud , Avanzi Charlotte , Busso Philippe , Benjak Andrej , Sales Anna Maria , Ferreira Cássio Porto , de Berrêdo-Pinho Márcia , Cole Stewart Thomas , Sarno Euzenir Nunes , Moraes Milton Ozório , Pinheiro Roberta Olmo 
Published: July 2022

Multidrug therapy (MDT) has been successfully used in the treatment of leprosy. However, although patients are cured after the completion of MDT, leprosy reactions, permanent disability, and occasional relapse/reinfection are frequently observed in patients. The immune system of multibacillary patients (MB) is not able to mount an effective cellular immune response against M. leprae. Consequently, clearance of bacilli from the body is a slow process and after 12 doses of MDT not all MB patients reduce bacillary index (BI). In this context, we recruited MB patients at the uptake and after 12-month of MDT. Patients were stratified according to the level of reduction of the BI after 12 doses MDT. A reduction of at least one log in BI was necessary to be considered a responder patient. We evaluated the pattern of host gene expression in skin samples with RNA sequencing before and after MDT and between samples from patients with or without one log reduction in BI. Our results demonstrated that after 12 doses of MDT there was a reduction in genes associated with lipid metabolism, inflammatory response, and cellular immune response among responders (APOBEC3A, LGALS17A, CXCL13, CXCL9, CALHM6, andIFNG). Also, by comparing MB patients with lower BI reduction versus responder patients, we identified high expression ofCDH19, TMPRSS4, PAX3, FA2H, HLA-V, FABP7, andSERPINA11before MDT. From the most differentially expressed genes, we observed that MDT modulates pathways related to immune response and lipid metabolism in skin cells from MB patients after MDT, with higher expression of genes likeCYP11A1, that are associated with cholesterol metabolism in the group with the worst response to treatment. Altogether, the data presented contribute to elucidate gene signatures and identify differentially expressed genes associated with MDT outcomes in MB patients.
Proteomic Profile of Procoagulant Extracellular Vesicles Reflects Complement System Activation and Platelet Hyperreactivity of Patients with Severe COVID-19
Authors: Moraes Emilly Caroline dos Santos , Martins-Gonçalves Remy , Silva Luana Rocha da , Mandacaru Samuel Coelho , Melo Reynaldo Magalhães , Azevedo-Quintanilha Isaclaudia , Perales Jonas , Bozza Fernando A. , Souza Thiago Moreno Lopes , Castro-Faria-Neto Hugo Caire , Hottz Eugenio D. , Bozza Patricia T. , Trugilho Monique R. O. 
Published: July 2022

BackgroundExtracellular vesicles (EVs) are a valuable source of biomarkers and display the pathophysiological status of various diseases. In COVID-19, EVs have been explored in several studies for their ability to reflect molecular changes caused by SARS-CoV-2. Here we provide insights into the roles of EVs in pathological processes associated with the progression and severity of COVID-19.MethodsIn this study, we used a label-free shotgun proteomic approach to identify and quantify alterations in EV protein abundance in severe COVID-19 patients. We isolated plasma extracellular vesicles from healthy donors and patients with severe COVID-19 by size exclusion chromatography (SEC). Then, flow cytometry was performed to assess the origin of EVs and to investigate the presence of circulating procoagulant EVs in COVID-19 patients. A total protein extraction was performed, and samples were analyzed by nLC-MS/MS in a Q-Exactive HF-X. Finally, computational analysis was applied to signify biological processes related to disease pathogenesis.ResultsWe report significant changes in the proteome of EVs from patients with severe COVID-19. Flow cytometry experiments indicated an increase in total circulating EVs and with tissue factor (TF) dependent procoagulant activity. Differentially expressed proteins in the disease groups were associated with complement and coagulation cascades, platelet degranulation, and acute inflammatory response.ConclusionsThe proteomic data reinforce the changes in the proteome of extracellular vesicles from patients infected with SARS-CoV-2 and suggest a role for EVs in severe COVID-19.