bluuebunny/crossref_metadata_2025_split huggingface dataset:
Introducing the CRISPR/Cas9 cytosine base editor toolbox ‘LeishBASEedit’ – Gene editing and high-throughput screening inLeishmaniawithout requiring DNA double-strand breaks, homologous recombination or donor DNA
Authors: Beneke Tom , Engstler Markus 
Published: December 2022

ABSTRACTCRISPR/Cas9 gene editing has revolutionised loss-of-function experiments inLeishmania, the causative agent of leishmaniasis. AsLeishmanialack a functional non-homologous DNA end joining pathway however, obtaining null mutants typically requires additional donor DNA, selection of drug resistance-associated edits or time-consuming isolation of clones. Genome-wide loss-of-function screens across different conditions and across multipleLeishmaniaspecies are therefore unfeasible at present. Here, we report a CRISPR/Cas9 cytosine base editor (CBE) toolbox that overcomes these limitations. We employed CBEs inLeishmaniato introduce STOP codons by converting cytosine into thymine and createdwww.leishbaseedit.netfor CBE primer design in kinetoplastids. Through reporter assays and by targeting single- and multi-copy genes inL. mexicana,L. major, L. donovaniandL. infantum, we demonstrate how this tool can efficiently generate functional null mutants by expressing just one single-guide RNA, reaching up to 100% editing rate in non-clonal populations. We then generated aLeishmania-optimised CBE and successfully targeted an essential gene in a plasmid library delivered loss-of-function screen inL. mexicana. Since our method does not require DNA double-strand breaks, homologous recombination, donor DNA or isolation of clones, we believe that this enables for the first time functional genetic screens inLeishmaniavia delivery of plasmid libraries.
M-commerce adoption in Chinese family businesses: does IT investment matter?
Authors: Hooi Lai Wan 
Published: December 2022

PurposeThis study aims to investigate the relationship between all three dimensions of perceived strategic value of m-commerce (operational support, managerial productivity and strategic decision aids), antecedents of m-commerce (organizational readiness, external context and m-commerce competence) and m-commerce adoption. The present study will further examine a mediation model in which all three dimensions of perceived strategic value of m-commerce affect m-commerce adoption through IT investment.Design/methodology/approachAn online survey questionnaire was adopted to test the validity of this research and hypotheses. Data were collected from 178 Chinese family businesses via snowball sampling.FindingsThe results show that all three dimensions of perceived strategic value of m-commerce (operational support, managerial productivity and strategic decision aids) are positively connected to m-commerce adoption. Also, it was found that IT investment partially or fully mediates the relationship between all these dimensions of perceived strategic value of m-commerce and m-commerce adoption.Originality/valueThis study would enhance owners' and managers' understanding of the relationship between perceived strategic value of m-commerce, IT investment, antecedents of m-commerce and m-commerce adoption, thus contributing to their future adoption.
Dubai Electricity and Water Authority (DEWA): a case study on sustainability
Authors: Suri Amjad , Al-Hadrami Abdullah , Sarea Adel , ElAsad Ali 
Published: December 2022

PurposeThe main purpose of the Dubai Electricity and Water Authority (DEWA) sustainability case is to allow students to explore how nonfinancial information reported in sustainability plays a vital role in maintaining a trade-off between current economic pressure and future environmental needs.Design/methodology/approachThis is an exploratory study in nature using a qualitative case study approach. The case requires an examination of DEWA's sustainability reporting (SR) in the context of Global Reporting Initiatives (GRIs). This case is designed to assist students in gauging DEWA's sustainability and explore how the company evaluates the materiality of sustainability issues.FindingsWith stakeholders' and investors' increased interest in sustainability, the authors argue that accounting programs should incorporate this topic into their curricula. The case enables students to focus on sustainability-related initiatives with DEWA that are aligned with GRI initiatives. The case might be instructive for both undergraduate and postgraduate students studying environmental and management accounting.Originality/valueThis case study is the first of its kind in the Gulf Cooperation Council (GCC) region to comprehensively analyze DEWA's sustainability practices concerning GRI-based SR. This study widens the understanding of DEWA's implementation of GRI standards in the preparation of its sustainability reports.
How organizational management supports instructional leadership
Authors: Shaked Haim 
Published: December 2022

PurposePrevious studies found that principals must be involved in both instructional leadership and organizational management, yet they did not explain how the former supports the latter. To narrow this gap in the available research-based knowledge, the current study explored the contribution of organizational management to instructional leadership.Design/methodology/approachThis study was qualitative in nature. The study participants were 28 principals of elementary schools in Israel. Data were collected through semi-structured interviews. Data analysis, based on coding, aimed to cluster the eight organizational management functions according to how each function supports instructional leadership.FindingsThe present study revealed that the eight functions of organizational management support four main aspects of instructional leadership: (1) Developing a positive learning climate; (2) Improving teaching quality; (3) Realizing the school instructional vision; and (4) Enabling instructional leadership.Originality/valueThe findings of this study reinforce the argument that although instructional leadership is the critical component of effective school leadership, it should be supported by other frameworks.
Analysis of performance of Covid-19 vaccine supply chain in India
Authors: Nagarajan Chandrasekaran , A. Indira , M. Ramasubramaniam 
Published: December 2022

Purpose
This study aims to analyse the structure of the Indian vaccine supply chain (SC) during the Covid-19 crisis and explore the underlying challenges at each stage in the network. It also brings out the difference in performance of various constituent states.

Design/methodology/approach
This study relied on both primary and secondary data for the analyses. For the primary data, the study gathered experts’ opinions to validate the authors’ inferences. For the secondary data, it relies on government data provided in websites.

Findings
Based on the quartile analysis and cluster analysis of the secondary data, the authors find that the constituent states responded differently during the first and second waves. This was due to the differences in SC characteristics attributed to varied demographics and administrative efficiency.

Research limitations/implications
This paper’s analyses is primarily limited to secondary information and inferences are based on them. The study has important implications for implementing the large-scale vaccination drives by government and constituent states for better coordination and last-mile delivery.

Originality/value
The contribution is unique in studying the performance of constituent states using statistical techniques, with secondary data from authentic sources. It is also unique in combining this observation with validation from experts.
A scoping review of the literature of interventions for enhancing nurses' resilience in acute care settings
Authors: Albsoul Rania Ali , Hughes James , Alshyyab Muhammad Ahmed , Fitzgerald Gerard 
Published: December 2022

PurposeThe aim of this study was to identify and evaluate interventions shown to improve nurse resilience in the acute care settings.Design/methodology/approachThe study was a systematically conducted scoping review of the literature. Databases including MEDLINE/PubMed, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Emerald insight and Google Scholar were searched and this complemented by reviews of the reference lists.FindingsIn total, 13 papers were included in the review but there was limited evidence of the effectiveness of individual programs. The authors found that resilience training programs for individuals reviewed provided some evidence for the effectiveness of the training. However, the context of job design, work risks and leadership require attention.Originality/valueResilience is an important requirement for nursing staff that helps to mitigate the stress of the working environment, particularly in the acute care setting. However, the managerial strategies required to build resilience are not well known or applied. The findings of this research may help to design cohesive and comprehensive management programs to promote and preserve nursing resilience in acute care settings. Any such program needs to reflect the four key themes that appear to underpin resilience: relationships, motivation, emotions and well-being.
The spatial (in)stability of mental health calls for police service
Authors: Koziarski Jacek 
Published: December 2022

AbstractResearch summaryInspired by studies on crime concentration, scholars have begun examining the spatial patterns of other issues under the police mandate, such as calls for service involving persons with perceived mental illness (PwPMI). While findings show that PwPMI calls for service concentrate in a few number of places, we do not know whether the concentration of these calls fall within a narrow bandwidth of spatial units nor whether these calls are spatially stable. Drawing on 7 years of calls for service data from the Barrie Police Service, this study tests for the temporal stability of PwPMI call for service concentrations at two units of spatial analysis and applies a longitudinal variation of the Spatial Point Pattern Test to assess the spatial stability of these calls at both the global and local levels. The results reveal that concentrations of PwPMI calls for service not only fall within a narrow proportional bandwidth of spatial units, but are spatially stable, even during the COVID‐19 pandemic.Policy implicationsExisting police‐ and community‐based efforts that respond to PwPMI in the community are tasked with responding to crises that could have been prevented with timelier intervention. Drawing from crime‐focused, place‐based policing strategies whose deployment is informed by the spatial concentration of crime, scholars have similarly argued that knowledge on where PwPMI calls for service concentrate can be leveraged to inform and deploy place‐based efforts whose focus is to assist PwPMI in a proactive capacity. The findings of the present study further substantiates the deployment of PwPMI‐focused police‐ and community‐based resources as proactive, place‐based efforts. In doing so, these efforts could not only prevent mental health crises from occurring but could prevent future police‐involved calls for service and thus reduce the footprint of the police in the lives of PwPMI in a reactive capacity.
Prolonged peripheral seronegative spondyloarthritis following BioNTech coronavirus disease 2019 vaccination: A case report
Authors: Koh Shu‐Yi , Chen Hui‐Ming , Hsu Chung‐Yuan 
Published: December 2022

AbstractA female 17‐year‐old diagnosed with seronegative spondyloarthritis (SpA) following the first jab of the BioNTech162b2 (BNT162b2) vaccine presented with recurrent swelling and painful knee accompanied by posterior heel tenderness over the past 1.5 months. Laboratory investigations revealed elevated serum erythrocyte sedimentation rate and C‐reactive protein. Synovial aspiration yielded level 3 crystal‐free, aseptic and inflammatory effusion. She tested positive for the human leukocyte antigen‐B27 and was diagnosed with peripheral SpA. She received daily celecoxib (400 mg), methylprednisolone (8 mg), and sulfasalazine (2 g), but the effect was limited. Nonetheless, her symptoms improved significantly with weekly subcutaneous etanercept administration (50 mg). Four weeks later, her arthritis was completely resolved. To our knowledge, this is the first case report of newly diagnosed seronegative peripheral SpA in an autoimmunity‐disease‐free individual following messenger RNA BNT coronavirus disease 2019 vaccination.
Computed tomography‐guided localization of pulmonary nodules prior to thoracoscopic surgery
Authors: Wang Lixin , Sun Daqian , Gao Min , Li Chunhai 
Published: December 2022

AbstractWith the increasing awareness of physical examination, the detection rate of pulmonary nodules is gradually increasing. For pulmonary nodules recommended for management by video‐assisted thoracic surgery (VATS), preoperative localization of the nodule is required if its location is difficult to determine intraoperatively by palpation. The computed tomography (CT)‐guided preoperative localization technique is the most widely used method with low operational difficulty and high efficiency, which can include hook wire, microcoil, medical dye, medical surgical adhesive, combined application, and emerging localization techniques according to the material classification. Each method has its corresponding advantages and disadvantages, but there is still a lack of unified guidelines or standards for the selection of CT‐guided preoperative localization methods in clinical practice. This review summarizes the operation precautions, advantages, and shortcomings of the above localization techniques in order to provide references for clinical application.
Toxic epidermal necrolysis and Stevens‐Johnson syndrome after COVID‐19 infection and vaccination
Authors: Zou Henry , Daveluy Steven 
Published: December 2022

AbstractStevens‐Johnson Syndrome (SJS) is a rare but severe skin reaction characterized by blistering and peeling of the skin and ulcerations of mucous membranes; toxic epidermal necrolysis (TEN) is a subset of SJS characterized by the involvement of >30% of the skin. Though previously associated with drugs and infections, discussions on the association between TEN/SJS and COVID‐19 have been limited. We present a review of TEN/SJS after COVID‐19 infection and vaccination. Literature searches were conducted on PubMed and Google Scholar from 2019 to 8/2022. Thirty‐eight articles were selected based on subject relevance, and references within selected articles were also screened for relevance. As of 8/2022, there have been 34 published cases of TEN, SJS, and SJS‐TEN overlap after COVID‐19 infection and vaccination, including 12 cases after vaccination and 22 cases after infection. Multiple authors hypothesize that virotopes or excipients in COVID‐19 vaccines can activate T‐cells or cytokines to induce TEN/SJS. Meanwhile, some hypothesize that COVID‐19 infection induces immune activation that can trigger TEN/SJS or increase susceptibility to drug‐induced TEN/SJS. Treatments for post‐infection and post‐vaccination TEN/SJS vary significantly. We recommend remaining vigilant for this rare and severe potential complication.
Is there convergence amongst shadow economies? International evidence
Authors: Henriquez Mora Camila , Saunoris James W. 
Published: December 2022

AbstractThis paper examines convergence among cross‐country shadow economies. Using the Phillips‐Sul (2007, 2009) club convergence approach and data for over 150 countries from 1991 to 2017, the results show evidence against absolute convergence for all shadow economies; however, we find evidence of multiple convergence clubs. In particular, we find evidence for seven distinct convergence clubs and six divergent shadow economies. Each club is characterised by an increasingly larger shadow economy with the countries in club 1 having the largest shadow economy and those in club 7 having the smallest shadow economy. Using a two‐way fixed effects approach, we also find that the determinants of the shadow economy are somewhat conditional on the convergence club. The existence of multiple equilibria suggests that policy makers in their attempt to combat the shadow economy would benefit by considering the different transitional paths associated with the different convergence clubs.
Abdominal aortic aneurysm and exfoliation syndrome: A literature review comparing single site findings
Authors: Patil Ayesha , Conley Matthew , Paulson Chase , Pompoco Christian , Wallace Ryan , Swiston Cole , Ritch Robert , Curtin Karen , Wirostko Barbara 
Published: December 2022

AbstractSeveral studies have suggested a possible relationship between exfoliation syndrome (XFS) and abdominal aortic aneurysm (AAA). A systematic literature review was undertaken to investigate this potential association. The systematic literature review was performed according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta‐Analyses) guidelines. Clear definitions of XFS and AAA were used to identify eligible studies via an unrestricted search of the PubMed interface from 1979 to October 31st, 2021. After review, 876 citations were gathered and evaluated for inclusion, from which 22 articles were included. Of these 22, 16 were excluded because they did not assess the relationship between AAA and XFS or provide primary data. Ultimately, six studies were included in this literature review. Half of the studies explored AAA prevalence in a population with or without XFS, and the other half explored the opposite. Three studies supported XFS as a risk factor for the development of AAA, and the other three found this relationship to be inconclusive. This systematic review revealed inconsistent results regarding an association between AAA and XFS. A large database study including XFS and AAA patients would be useful in further determining if an association does in fact exist.
Development and validation of the Work–Home Integration Questionnaire (WHIQ)
Authors: Noja Andrea , Kubicek Bettina , Plohl Nejc , Tement Sara 
Published: December 2022

AbstractThe boundaries between work and private life are gradually blurring. More and more employees are involved in work during leisure time through cognitions, emotions or behaviours, in both negative and positive ways. This so‐called work‐home integration (WHI) can, on the one hand, hampers the necessary recovery from work and result in strain but, on the other hand, also restores resources and result in beneficial outcomes. In order to enhance our understanding of WHI and capture all its different forms, we suggest a new conceptualisation and measure of WHI. We therefore developed and validated the Work‐Home Integration Questionnaire (WHIQ) in English, German and Slovene simultaneously using two cross‐sectional studies (Study 1: N = 848; Study 2: N = 555) and a two‐wave longitudinal study with a time lag of 1 month (Study 3: N = 379). Confirmatory factor analysis confirmed a three‐factor structure with (1) negative cognitive‐affective involvement, (2) positive cognitive‐affective involvement and (3) behavioural involvement. Moreover, the WHIQ showed measurement invariance across the three languages and the results provide evidence for convergent, discriminant and incremental validity. Overall, the WHIQ is a reliable, valid and short measure to assess the extent to which employees are involved in work during leisure time.
The silent epidemic: Death by suicide among physicians
Authors: Bhatia Gayatri , Sharma Pawan , Pal Arghya , Parmar Arpit 
Published: December 2022

AbstractSuicidal deaths among physicians have been on a steady rise in the past few decades, despite being a part of the healthcare system, training for early identification and easy access to treatment services. While there is no doubt that this warrants concern at individual, institutional, and community levels, physician suicide remains an under‐researched topic. We examine the correlates of suicidal deaths among physicians along with risks and protective factors conferred to physicians as a population and emphasize the need for preventive and risk‐reduction initiatives that are specifically tailored for physicians and the healthcare provider community.
Tensor Response Quantile Regression with Neuroimaging Data
Authors: Wei Bo , Peng Limin , Guo Ying , Manatunga Amita , Stevens Jennifer 
Published: December 2022

Abstract
Collecting neuroimaging data in the form of tensors (i.e. multidimensional arrays) has become more common in mental health studies, driven by an increasing interest in studying the associations between neuroimaging phenotypes and clinical disease manifestation. Motivated by a neuroimaging study of post-traumatic stress disorder (PTSD) from the Grady Trauma Project, we study a tensor response quantile regression framework, which enables novel analyses that confer a detailed view of the potentially heterogeneous association between a neuroimaging phenotype and relevant clinical predictors. We adopt a sensible low-rank structure to represent the association of interest, and propose a simple two-step estimation procedure which is easy to implement with existing software. We provide rigorous theoretical justifications for the intuitive two-step procedure. Simulation studies demonstrate good performance of the proposed method with realistic sample sizes in neuroimaging studies. We conduct the proposed tensor response quantile regression analysis of the motivating PTSD study to investigate the association between fMRI resting-state functional connectivity and PTSD symptom severity. Our results uncover non-homogeneous effects of PTSD symptoms on brain functional connectivity, which cannot be captured by existing tensor response methods.
Correcting Delayed Reporting of COVID-19 Using the Generalized-Dirichlet-Multinomial Method
Authors: Stoner Oliver , Halliday Alba , Economou Theo 
Published: December 2022

AbstractThe COVID-19 pandemic has highlighted delayed reporting as a significant impediment to effective disease surveillance and decision-making. In the absence of timely data, statistical models which account for delays can be adopted to nowcast and forecast cases or deaths. We discuss the four key sources of systematic and random variability in available data for COVID-19 and other diseases, and critically evaluate current state-of-the-art methods with respect to appropriately separating and capturing this variability. We propose a general hierarchical approach to correcting delayed reporting of COVID-19 and apply this to daily English hospital deaths, resulting in a flexible prediction tool which could be used to better inform pandemic decision-making. We compare this approach to competing models with respect to theoretical flexibility and quantitative metrics from a 15-month rolling prediction experiment imitating a realistic operational scenario. Based on consistent leads in predictive accuracy, bias, and precision, we argue that this approach is an attractive option for correcting delayed reporting of COVID-19 and future epidemics.