bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knockdown of <i>FLT4</i>, <i>Nup98</i>, and <i>Nup205</i> cellular genes as a suppressor for the viral activity of Influenza A/WSN/33 (H1N1) in A549 cell culture
Authors: Pashkov E. A. , Faizuloev E. B. , Korchevaya E. R. , Rtishchev A. A. , Cherepovich B. S. , Sidorov А. V. , Poddubikov A. V. , Bystritskaya Е. P. , Dronina Yu. E. , Bykov A. S. , Svitich O. А. , Zverev V. V. 
Published: January 2022

Objectives. To evaluate the effect of cellular genes FLT4, Nup98, and Nup205 on the reproduction of the influenza A virus in A549 human lung cancer cell line.Methods. The work was carried out using the equipment of the center for collective use of the I.I. Mechnikov Research Institute of Vaccines and Sera (Russia). The virus-containing fluid was collected within three days from the moment of transfection and infection and the intensity of viral reproduction was assessed by viral titration and hemagglutination reaction. The viral RNA concentration was determined by real-time reverse-transcription polymerase chain reaction (RT-PCR). To calculate statistically significant differences between groups, the nonparametric Mann–Whitney test was used.Results. In cells treated with small interfering RNAs (siRNAs) targeted at FLT4, Nup98, and Nup205 genes, a significant decrease in their expression and indicators of viral reproduction (virus titer, hemagglutinating activity, viral RNA concentration) was observed at a multiplicity of infection (MOI) = 0.1. Additionally, it was found that a decrease in the expression of target genes using siRNA does not lead to a significant decrease in cell survival. The viral titer in cells treated with siRNA FLT4.2, Nup98.1, and Nup205 on the first day was lower by an average of 1.0 lg, and on the second and third days, by 2.2–2.3 lg, compared to cells treated with nonspecific siRNA. During real-time RT-PCR, a significant decrease in the concentration of viral RNA was observed with siRNA Nup98.1 (up to 190 times) and Nup205 (up to 30 times) on the first day, 26 and 29 times on the second day, and 6 and 30 times on the third day, respectively. For FLT4.2 siRNA, the number of viral RNA copies decreased by 23, 18, and 16 times on the first, second, and third days. Similar results were obtained when determining the hemagglutinating activity of the virus. The hemagglutinating activity on the third day most strongly decreased in cells treated with siRNA Nup205 and FLT4.2 (16 times). In cells treated with siRNA FLT4.1, Nup98.1, and Nup98.2, hemagglutinating activity decreased by 8 times.Conclusions. In the present study, three cellular genes (FLT4, Nup98, and Nup205) were identified—the decrease in the expression of which effectively suppresses viral reproduction— and the original siRNA sequences were obtained. The results obtained are important for creating therapeutic and prophylactic medication, whose action is based on the RNA interference mechanism.
Foreign experience of legal regulation for separate offenses administrative liability and the possibility of its application in Ukraine
Authors: Kriepakova M. Yu. 
Published: January 2022

The scientific novelty of the study is that the research is one of the first works that analyzes foreign experience in legal liability for the involvement of a minor in the use of tobacco products, e-cigarettes, liquids used in e-cigarettes, devices for the consumption of tobacco products without their combustion, and uses the developed results to further improve the national legislation governing administrative liability in Ukraine. In accordance with the purpose and objectives of the study, the article uses a set of general theoretical and special scientific methods and techniques of scientific knowledge. Their use is due to a systematic approach, which makes it possible to explore problems in the unity of their social content and legal form. During the work on the article the methods of modeling, forecasting and others were used. The article analyzes the current state of legal regulation of administrative liability for involving a minor in antisocial behavior of such republics as Kazakhstan, Tajikistan, Belarus, Uzbekistan, Latvia. Based on the study, the ways of implementation of foreign experience in Ukraine are outlined, proposals for amendments to the Law of Ukraine "On state regulation of production and circulation of ethyl alcohol, cognac and fruit, alcoholic beverages, tobacco products, liquids used in electronic cigarettes and fuel" and the Code of Ukraine on Administrative Offenses, aimed at regulating the involvement of a minor in the use of tobacco products, electronic cigarettes, liquids used in electronic cigarettes, devices for consumption of tobacco products without their combustion, the implementation of which improves administrative legislation are formulated.
It is emphasized that these illegal actions against minors should entail legal liability. Addressing gaps in the legislation is one of the priority tasks facing administrative scientists.
DAMPAK COVID-19 PADA PEDAGANG KAKI LIMA (PKL) DI KOTA SERANG
Authors: Afrizal Stevany , Dewi Putri Tunggal 
Published: January 2022

Pandemi Covid-19 membawa banyak dampak dalam berbagai bidang kehidupan di Negara Indonesia, salah satunya adalah bidang ekonomi. Pada bidang ekonomi terjadinya penurunan pendapatan akibat sedikitnya jual beli yang dirasakan oleh semua golongan pada masyarakat. Tidak terkecuali Pedagang Kaki Lima (PKL) yang termasuk golongan informal juga merasakan dampak dari pandemi ini. Semenjak pemerintah mengeluarkan kebijakan Pembatasan Sosial Berskala Besar (PSBB) hal itu membuat para PKL kehilangan para pembelinya, yang berakibat pada menurunnya pendapatan. Tujuan dari penelitian ini adalah mendeskripsikan dampak covid-19 pada pedagang kaki lima (PKL) di Kota Serang agar bisa tetap bertahan ditengah pandemi covid-19 dan mendeskripsikan peraturan daerah yang mengatur tentang Ketertiban, Kebersihan, dan Keindahan (K3) di Kota Serang. Metode penelitian yang digunakan adalah kualitatif sifatnya deskriptif. Hasil dari penelitian ini adalah (1) Hilangnya pembeli dan pelanggan, karena ketika kebijakan PSBB diterapkan masyarakat lebih memilih untuk tetap di rumah dan enggan untuk keluar rumah jika tidak penting. (2) Menurunnya pendapatan, ini merupakan dampak lanjutan ketika pembeli berkurang, karena secara otomatis itu pun akan mengurangi pendapatannya juga. (3) PKL enggan untuk direlokasi karena takut kehilangan pelanggan dan tempat relokasi yang jauh dari keramaian masyarakat. Kata Kunci: Dampak, Pandemi Covid-19, dan Pedagang Kaki Lima
Gerenciamento de aquisições de um Projeto de uma Planta Industrial para tratamento de óleos e gorduras
Authors: Angeli Valcir Anibal , Tanomaru Julio , Santos Paulo Sergio Barbosa dos 
Published: January 2022

O gerenciamento de aquisições é fundamental para as organizações a fim de serem eficientes e eficazes nas contratações de produtos e serviços. Com a alta demanda por financiamentos as empresas buscam maior eficácia nas suas contratações, com isso a terceirizações dos serviços vem aumentando, as empresas procuram as áreas específicas com a terceirização de empresas especializadas garantindo assim o resultado desejado. O gerenciamento das aquisições trata da obtenção de produtos e serviços externos à equipe do projeto. Pode-se subdividir em quatro subprocessos: planejar o gerenciamento das aquisições; conduzir as aquisições; controlar as aquisições e encerrar as aquisições. Deve-se garantir que todas as etapas trabalhem interligadas. O presente estudo tem como objetivo principal abordar sobre a gestão de aquisições no gerenciamento de projetos, através do estudo de caso para a implantação de um processo industrial de tratamento de óleos e gorduras. Demostra assim a aplicação das técnicas do gerenciamento de aquisições em um estudo de caso para mostrar o sucesso da gestão, bem como na tradução dos objetivos estratégicos da organização em projetos para que, de fato, os resultados almejados sejam alcançados. Conclui-se que o que tem sido realizado em campo pela organização está seguindo as boas práticas do guia PMBOK, utilizando todos os processos de planejar, conduzir, controlar e encerrar e com isso tem demostrado que a eficiência no gerenciamento de aquisições tem beneficiado o projeto como todo.
Evaluación del impacto de la capacitación a directivos de la provincia de Guantánamo
Authors: Thaureaux Mirna Quiala , Cuhema Salvador L. Bernardo 
Published: January 2022

Las organizaciones empresariales se enfrentan en estos tiempos a cambios acelerados que se producen en todos los ámbitos de la sociedad; razón por lo cual la capacitación de los recursos humanos - y especialmente de los que dirigen - con enfoque sistémico, es una condición imprescindible para elevar su calificación directiva especializada. Esto presupone que la evaluación de la capacitación constituya un recurso para validar su perfeccionamiento constante y de esa forma distinguir puntualmente la situación que presenta verificando el cumplimiento de los objetivos, los resultados, efectos e impactos. Por estas razones la investigación realizada tuvo como objetivo la elaboración de un sistema de indicadores para evaluar el impacto de la capacitación a directivos en la Provincia Guantánamo, teniendo como base el Diplomado de Gerencia Empresarial y Administración Pública, el cual resultó evaluado de alto impacto una vez aplicado lo planteado. Esta investigación constituye un material de consulta para profesores, metodólogos y directivos porque en ella se ofrecen instrumentos para evaluar el impacto de la capacitación de los directivos a partir de un sistema de indicadores. Los métodos de investigación empleados fueron: analítico-sintético, inductivo-deductivo, histórico-lógico, estadístico-matemático, las encuestas, el estudio de documentos, y el criterio de expertos, los cuales posibilitaron evaluar las tendencias y enfoques existentes referidos al tema objeto de estudio; así como enriquecer, completar y contrastar información sobre el impacto de la preparación recibida. Igualmente permitió valorar la coherencia y factibilidad de la propuesta de indicadores para su utilización con el fin de perfeccionar la capacitación como proceso.
Assistência à saúde de adolescentes de minorias sexuais e de gênero: scoping review
Authors: Zanchetta Sabrina Brombim , Silveira Edilene Aparecida Araújo da 
Published: January 2022

Objetivo: Mapear as evidências cientificas sobre assistência em saúde aos adolescentes de minorias sexuais e de gênero no contexto da atenção primária. Metodologia: Scoping Review, que foi desenvolvida com base nas recomendações do guia internacional Joanna Briggs Institute Reviewers. Foi realizada busca em bases de dados selecionadas que continham artigos na íntegra e que respondessem ao questionamento: quais seriam os conhecimentos ou evidências científicas que poderiam nortear a elaboração de protocolos e ações educativas no contexto da atenção primária em saúde? Foram realizadas buscas em seis bases de dados internacionais e nacionais, sobre trabalhos publicados até 2021. Resultados: Dentre os 394 estudos, 25 foram selecionados para leitura na integra com uma amostra final de 11 estudos. Os 11 estudos foram publicados de 2017 a 2020. Os estudos selecionados são de âmbito internacional e de abordagem quantitativa, qualitativa e qualiquantitativa. Os estudos trouxeram importantes propostas de intervenções que podem ser realizadas para melhorar o atendimento de profissionais no ambiente da atenção primária. Conclusão: Percebe-se que o campo de estudo é pouco explorado pela literatura nacional e os estudos são observacionais, ocorrendo uma lacuna em relação a diversidade de atendimento para minorias sexuais e de gênero que deve ser preenchida por estudos futuros.
Immobilization and Evaluation of Penicillin G Acylase on Hydroxy and Aldehyde Functionalized Magnetic α-Fe2O3/Fe3O4 Heterostructure Nanosheets
Authors: Ni Yun , Lv Zhixiang , Wang Zhou , Kang Shouyu , He Dawei , Liu Ruijiang 
Published: January 2022

Magnetic α-Fe2O3/Fe3O4 heterostructure nanosheets were fabricated via hydrothermal calcination. The activity of penicillin G acylase (PGA), which was covalently immobilized onto silica-decorated heterostructure nanosheets, achieved the highest activity of 387.03 IU/g after 18 h of incubation with 0.1 ml of PGA. In contrast, the activity of free PGA reached the highest level when the temperature was 45°C with a pH of 8.0. However, the activity of free PGA changed more dramatically than immobilized PGA as the relative conditions changed. Moreover, the Michaelis–Menten constant (Km) and reusability of immobilized PGA were also explored. The results showed that free PGA Km and maximum rate (Vmax) were 0.0274 M and 1.167 μl/min, respectively. Km and Vmax values of immobilized PGA were 0.1082 M and 1.294 μl/min, respectively. After 12 cycles of repetitive use, immobilized PGA remained approximately 66% of its initial activity, indicating that the PGA immobilized onto the heterostructure nanosheets showed better stability and reusability than free PGA.
Acute Effects of Heel-to-Toe Drop and Speed on Running Biomechanics and Strike Pattern in Male Recreational Runners: Application of Statistical Nonparametric Mapping in Lower Limb Biomechanics
Authors: Yu Peimin , He Yuhuan , Gu Yaodong , Liu Yuwei , Xuan Rongrong , Fernandez Justin 
Published: January 2022

With the increased popularity of running, many studies have been conducted into footwears that are highly related to running performance and running-related injuries. Previous studies investigated different shoe types and running shoes with different heel-to-toe drops (HTDs). However, no research was found in investigating shoes with negative values with HTD. Therefore, the aim of this study was to determine the acute effect of HTD and running speed on lower limb biomechanics and strike pattern in recreational runners. Thirteen male recreational runners wearing shoes with two different HTDs (−8 and 8 mm) performed running at three different speeds (preferred speed [PS], 90% of PS, 110% of PS). Lower extremity kinematics and ground reaction forces were synchronously captured via Vicon motion analysis system and AMTI force platform. Strike index (SI), vertical average loading rate (VALR), vertical instantaneous loading rate (VILR), excursion, eversion duration, joint angles, and range of motion (ROM) of metatarsophalangeal (MTP), ankle, knee, and hip joints were calculated. Joint angles during the entire stance phase were analyzed applying the statistical nonparametric mapping (SnPM) method. SI and VILR in shoes with −8 mm HTD significantly increased by 18.99% and 31.836 BW/s compared to those with 8 mm HTD (SI: p = 0.002; VILR: p &lt; 0.001). Significant alterations of ROM occurred in the MTP, ankle, and knee joints (p &lt; 0.05), and HTD factor primarily accounted for these changes. Joint angles (MTP, knee, and hip) during the entire stance phase altered due to HTD and speed factors. Running speed primarily influenced the kinematics parameters of knee and hip joints, increasing knee angles in the frontal plane and hip angle in the horizontal plane at PS (p &gt; 0.05). Compared to shoes with 8 mm HTD, shoes with −8 mm HTD may be useful to storage and return energy because of the increased ROM of MTP in the sagittal plane. Besides, forefoot strike gait retraining was recommended before transition from normal running shoes to running shoes with −8 mm HTD.
Sa12b Improves Biological Activity of Human Degenerative Nucleus Pulposus Mesenchymal Stem Cells in a Severe Acid Environment by Inhibiting Acid-Sensitive Ion Channels
Authors: Wang Ziyu , Han Letian , Chen Haoyu , Zhang Shengquan , Zhang Sumei , Zhang Hua , Li Yuhao , Tao Hui , Li Jie 
Published: January 2022

Sa12b is a wasp peptide that can inhibit acid-sensitive ion channels (ASICs). The biological effects of nucleus pulposus mesenchymal stem cells (NP-MSCs) have not been investigated. Therefore, this study investigated the effect of Sa12b on the biological activity of NP-MSCs through ASICs in the acidic environment of intervertebral disc degeneration (IVDD). In this study, NP-MSCs were isolated from the nucleus pulposus (NP) in patients who underwent lumbar disc herniation surgery, identified by flow cytometry and tertiary differentiation, and cultured in vitro in an acidic environment model of IVDD with a pH of 6.2. Proliferation, and apoptosis were observed after different Sa12b concentrations were added to P2 generation NP-MSCs. The Ca2+ influx was detected using flow cytometry and laser confocal scanning microscopy, and qPCR was used to detect the relative expression of stem cell–associated genes (Oct4, Nanog, Jag1, and Notch1), the relative expression of extracellular matrix (ECM)–associated genes (collagen II, aggrecan, and SOX-9), and the relative expression of genes encoding ASICs (ASIC1, ASIC2, ASIC3, and ASIC4). Western blotting was used to detect the protein expression of collagen II and aggrecan in different treatment groups. Cells isolated and cultured from normal NP were spindle-shaped and adherent, and they exhibited expansion in vitro. Flow cytometry results showed that the cells exhibited high expression of CD73 (98.1%), CD90 (97.5%), and CD105 (98.3%) and low expression of HLA-DR (0.93%), CD34 (2.63%), and CD45 (0.33%). The cells differentiated into osteoblasts, adipocytes, and chondrocytes. According to the International Society for Cellular Therapy criteria, the isolated and cultured cells were NP-MSCs. With an increase in Sa12b concentration, the cell proliferation rate of NP-MSCs increased, and the apoptosis rate decreased significantly, reaching the optimal level when the concentration of Sa12b was 8 μg/μl. When the Sa12b concentration was 8 μg/μl and contained the ASIC non-specific inhibitor amiloride, the Ca2+ influx was the lowest, followed by that when the Sa12b concentration was 8 μg/μl. The Ca2+ influx was the highest in the untreated control group. qPCR results showed that as the concentration of Sa12b increased, the relative expression of Oct4, Nanog, Jag1, Notch1, collagen II, aggrecan, and SOX-9 increased, while that of ASIC1, ASIC2, ASIC3, and ASIC4 decreased. The difference was statistically significant (p &lt; 0.05). In conclusion, Sa12b can improve the biological activity of NP-MSCs in severely acidic environments of the intervertebral disc by reducing Ca2+ influx via AISC inhibition and, probably, the Notch signaling pathway. This study provides a new approach for the biological treatment of IVDD. Inhibition of AISCs by Sa12b may delay IVDD and improve low back pain.
Soluble Urokinase Plasminogen Activator Receptor Is Associated With Subclinical Myocardial Impairment by Speckle Tracking Echocardiography in Lung Cancer Patients
Authors: Manshad Ahmad S. , Ballout Fatima A. , Borgia Jeffrey A. , Reiser Jochen , Okwuosa Tochukwu M. 
Published: January 2022

BackgroundPlasma cardiac biomarkers have emerged as a cost-effective diagnostic tool aimed at early identification of cardiotoxicity. Soluble urokinase plasminogen activator receptor (suPAR) is a bone marrow cell derived signaling molecule that is associated with cardiovascular disease outcomes.ObjectivesWe investigated associations between suPAR and global longitudinal strain (GLS) as a marker of early myocardial impairment in lung cancer patients.MethodsWe retrospectively analyzed 52 patients with stage IV non-small cell lung cancer with normal left ventricular ejection fraction (LVEF &gt;55%) and without known heart disease or end-stage renal disease (ESRD). We studied associations between cardiac biomarkers and echocardiographic measures of systolic and diastolic function. GLS was analyzed using 2D speckle-tracking echocardiography via vendor-independent software (TomTec).ResultsMedian plasma suPAR was 7.0 ng/mL (interquartile range: 5.4–9.0). Mean LVEF was 61.9 ± 8.3% and mean GLS was-19.3 ± 2.1%. Inter-observer reproducibility was excellent for GLS as determined by Intraclass Correlation Coefficient analysis, ICC = 0.81 (0.68–0.89). After multivariate analysis, suPAR was the only biomarker associated with GLS (p = 0.009). suPAR was also associated with diastolic parameters E velocity (p = 0.018), A velocity (p = 0.017), and E/E' ratio (p = 0.033). Interestingly, suPAR was not associated with LVEF (p = 0.916). In addition, suPAR and GLS were found to be age-independent predictors of all-cause mortality, though only GLS remained significant after multivariate adjustment.ConclusionsIn this cohort of stage IV non-small cell lung cancer patients with normal LVEF and without known heart disease or ESRD, suPAR was associated with GLS and diastolic impairment. suPAR is a readily available inexpensive biomarker; further research is required to evaluate the possible role of suPAR in screening for subclinical LV dysfunction in the high-risk oncological population.
A Predictive Scoring Model for Postoperative Tracheostomy in Patients Who Underwent Cardiac Surgery
Authors: Wang Dashuai , Wang Su , Du Yifan , Song Yu , Le Sheng , Wang Hongfei , Zhang Anchen , Huang Xiaofan , Wu Long , Du Xinling 
Published: January 2022

BackgroundA subset of patients require a tracheostomy as respiratory support in a severe state after cardiac surgery. There are limited data to assess the predictors for requiring postoperative tracheostomy (POT) in cardiac surgical patients.MethodsThe records of adult patients who underwent cardiac surgery from 2016 to 2019 at our institution were reviewed. Univariable analysis was used to assess the possible risk factors for POT. Then multivariable logistic regression analysis was performed to identify independent predictors. A predictive scoring model was established with predictor assigned scores derived from each regression coefficient divided by the smallest one. The area under the receiver operating characteristic curve and the Hosmer-Lemeshow goodness-of-fit test were used to evaluate the discrimination and calibration of the risk score, respectively.ResultsA total of 5,323 cardiac surgical patients were included, with 128 (2.4%) patients treated with tracheostomy after cardiac surgery. Patients with POT had a higher frequency of readmission to the intensive care unit (ICU), longer stay, and higher mortality (p &lt; 0.001). Mixed valve surgery and coronary artery bypass grafting (CABG), aortic surgery, renal insufficiency, diabetes mellitus, chronic obstructive pulmonary disease (COPD), pulmonary edema, age &gt;60 years, and emergent surgery were independent predictors. A 9-point risk score was generated based on the multivariable model, showing good discrimination [the concordance index (c-index): 0.837] and was well-calibrated.ConclusionsWe established and verified a predictive scoring model for POT in patients who underwent cardiac surgery. The scoring model was conducive to risk stratification and may provide meaningful information for clinical decision-making.