bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential neuromuscular advice for pathologists: Limb Girdle Muscular Dystrophy (second of two parts)
Authors: Cotta Ana , Carvalho Elmano , da-Cunha-Júnior Antonio Lopes , Antunes Julio Salgado , de Souza Francineide Sadala , de Moura Heveline Becker , de Brito Pinto Andreia Portilho , Valicek Jaquelin , Navarro Monica Machado , Godinho Frederico , da Silveira Eni Braga , Lima Maria Isabel , Cordeiro Bruno Arrivabene , Cauhi Alexandre Faleiros , Menezes Miriam Melo , Nunes-Neves Simone Vilela , Vargas Antonio Pedro , da-Silva-Neto Rafael Xavier , Giron Renata Lobo , Costa-e-Silva Cynthia , Takata Reinaldo Issao , Paim Julia Filardi 
Published: February 2025

Abstract

Background
Limb Girdle Muscular Dystrophy is defined as a group of progressive autosomal recessive (85%, 28 genes) and autosomal dominant (15%, 5 genes) muscular dystrophies described in at least two unrelated families, affecting individuals that achieve independent walking, with predominant proximal muscles weakness at presentation, elevated serum creatine kinase activity, dystrophic changes on muscle biopsy, and degeneration on muscle imaging over the course of the disease.

Main body
The aims of this review are: (1) to show the recent Limb Girdle Muscular Dystrophy (LGMD) genetic classification illustrated with clinical and physiopathological characteristics, and cellular localization of the main gene products; (2) to present muscle radiophenotypes with an algorithm for differential diagnosis; (3) to show the role of muscle biopsy for phenotypic characterization, and pathogenicity confirmation in the era of surgical-molecular pathology.

Conclusion
Pathologists may be aware of clinical, neurophysiological, laboratorial, imaging, molecular, and muscle biopsy modalities to provide a precise phenotypic-genotypic diagnosis for adequate rehabilitation care, and genetic counselling.
FOXF2 rs41300825 and NOTCH3 rs1043994 as risk loci for cerebral small vessel disease in Egyptian ischemic stroke patients
Authors: Hussein Fatma Salama , Sayed Soha Saad Eldin , Mohammed Hossam Shokri , Kamal Maha Moustafa 
Published: February 2025

Abstract

Background
Cerebral small vessel disease (CSVD) is a significant cause of ischemic stroke in all ethnic groups. Genetic determinants of CSVD are poorly understood and have rarely been explored. One proposed mechanism behind SVD is the breakdown of the blood–brain barrier (BBB). An intact barrier relies heavily on appropriate pericyte (PC) coverage of BBB endothelial cells. FOXF2 and NOTCH3 are two genes implicated in PC development and maintenance, thus influencing PC coverage of brain blood vessels. The aim of this study is to investigate whether FOXF2 rs41300825 and NOTCH3 rs1043994 variants can be considered as candidate loci for CSVD in Egyptian patients, marking the first study of its kind in Egypt.
The present study included 186 ischemic stroke patients, selected from neurology and psychiatry department at Ain Shams University hospitals. Patients were categorized into: Group I (SVD patients; n = 114) and Group II (LVD patients; n = 72). Genotyping was conducted for FOXF2 rs41300825 (G/C) and NOTCH3 rs1043994 (A/G/T) using RFLP-PCR, with results confirmed through Sanger sequencing and multiple alignments of sequencing using the MEGA X program.

Results
Results showed that genotype analysis for FOXF2 rs41300825 displayed one band of 334 bp, indicating the GG genotype. The same was observed for NOTCH3 rs1043994 with one band of 665 bp, representing the GG genotype. Sanger sequencing and multiple alignments of sequencing using the MEGA X program confirmed the results.

Conclusion
Neither FOXF2 rs41300825 nor NOTCH3 rs1043994 were able to differentiate between patients at risk of developing SVD and those susceptible to LVD. Further studies with larger sample sizes are necessary.
Mapping and Forgetting
Authors: Jaguaribe Beatriz 
Published: February 2025

Abstract
Centered on the mapping of the Brazilian state of Mato Grosso, this essay explores the relations between cartography, national imaginaries, and personal archives. Undertaken in 1917 by the Rondon Commission during the period of the First Republic (1889–1930), the making of the map spanned decades of Brazilian history, as it was finally published in 1952 during Getúlio Vargas's democratic presidency. Its making entailed the exploration of the vast Brazilian hinterlands, the transformation of Indigenous peoples, and the shaping of the lives of the explorers, guides, and cartographers engaged in the enterprise. Since its beginning, the making of the map was directed by Cândido Mariano da Silva Rondon's close collaborator, the military cartographer and chief of the cartography section of the Rondon Commission, Francisco Jaguaribe. As Jaguaribe was the author's paternal grandfather, the effort of mapping what was then the immense terrain of Mato Grosso became embedded in the memorial archives of her family. The author envisions the map as a material object, as a repertoire of imaginaries, a monument, ruin, and allegory. In narrating its saga, the author seeks to entwine personal lives with national projects, individual/collective oblivion with national erasure, and mapping with experiences of creation and destruction.
Management of Major Arterial Injuries: A Critical Complication of Endoscopic Endonasal Surgery
Authors: Emengen Atakan , Yilmaz Eren , Gokbel Aykut , Uzuner Ayse , Cakir Ozgur , Ciftci Ercument , Ozsoy Bedrettin , Ergen Anil , Caklili Melih , Cabuk Burak , Anik Ihsan , Ceylan Savas 
Published: February 2025

BACKGROUND AND OBJECTIVES:
Endoscopic endonasal surgery (EES) is the preferred approach for treating sellar and parasellar lesions due to its panoramic view and detailed anatomical definition. This study aims to review major arterial injuries during EES, a rare but critical complication associated with high mortality and morbidity rates.

METHODS:
A retrospective analysis was conducted, including 15 patients from 5640 EES procedures performed between August 1997 and February 2024 at in our center/Department of Neurosurgery. The study focuses on perioperative and postoperative management strategies, highlighting the use of oxidized regenerated cellulose (Surgicel®)/gelatin-thrombin matrix (Floseal®) and multidisciplinary collaboration to achieve hemostasis.

RESULTS:
The incidence of major arterial injury was 0.2%, with a mean patient age of 38.4 years and a female-to-male ratio of 10:5. The most common tumors were adenomas (n = 7) and chordomas (n = 5). The most commonly bleeding arteries in 6 patients each are the cavernous segment of the internal carotid artery and the paraclival carotid artery. Low-flow bleeding was observed in 10 of our patients, while high-flow bleeding was seen in 5 patients, 4 of whom were recurrent cases (P < .05). In 3 patients with high-flow bleeding from segments of the carotid artery, digital subtraction angiography was performed for stent placement or coil embolization, while in 1 patient with high-flow bleeding, digital subtraction angiography was used for monitoring cerebral blood flow. One of our patients died of a myocardial infarction at the 14th month of follow-up and 1 of our patients developed left-hemiparesis.

CONCLUSION:
The study underscores the importance of preoperative planning, surgical expertise, and multidisciplinary teamwork in managing major arterial injuries during EES. The authors advocate for the development of standardized treatment algorithms to improve outcomes in these patients. The findings contribute to the limited literature on managing internal carotid artery injuries in EES, emphasizing the need for ongoing research and algorithm development.
How a salt pan ant Cataglyphis fortis navigates artificially complex environments
Authors: Freire Marilia , Oberweiser Morgan M. , Bollig Antonio , Kunert Grit , Knaden Markus 
Published: February 2025

ABSTRACT
The desert ant Cataglyphis fortis inhabits the harsh and featureless North African saltpans. Individuals forage long distances and return to their inconspicuous nest entrance using path integration, but also rely on visual and olfactory landmarks. Here, we investigated the navigational decision making of these ants in differently structured environments. While individual ants show consistent route preferences, significant variability exists between individuals. Furthermore, the ants favor repetitive routes, suggesting that vision-based learning mechanisms and motor responses guide their navigation, with similar visual cues leading to similar egocentric decisions. This formation of idiosyncratic routes, seen in other ant species, appears to be conserved in C. fortis despite its usually flat habitat.
Crossover Point Differences in Combat Sport Athletes and Runners during Exercise Testing
Authors: Stanzione Joseph R. , Brooks George A. , Bruneau Michael L. , French Duncan N. , Nasser Jennifer A. , Smith Sinclair A. , Ramadoss Rohit , Volpe Stella L. 
Published: February 2025

ABSTRACT

Introduction
During exercise, substrate utilization is characterized by “crossover point,” a term representing the predominant transition from fat to carbohydrate. Exercise intensity highly influences crossover point, but there is a paucity of research on sport-specific differences. The purpose of this study was to examine differences in time to crossover, heart rate (HR), and percent maximal oxygen consumption (%V˙O2max) at crossover point between combat athletes and runners during maximal and submaximal tests. Differences in blood lactate, ketone, and glucose responses between sports were also analyzed.

Methods
Eight male combat athletes and 18 runners were measured for respiratory exchange ratio (RER) during V˙O2max and submaximal treadmill tests. A Kruskal–Wallis test was applied to determine differences between sport types for time to crossover during both tests. A two-way mixed factorial analysis of variance (ANOVA) was applied, including sport type and exercise test as the between- and within-group factors, respectively. The dependent variables included HR and %V˙O2max at crossover. For capillary sampling, a two-way mixed ANOVA was applied with blood lactate, ketone, and glucose concentrations as dependent variables. Alpha levels were determined a priori at P < 0.05.

Results
A significant difference was observed between combat athletes and runners (average age: 32.5 ± 12 yr) in time to crossover point during the submaximal test (P = 0.009). Combat athletes reached crossover point earlier (10:03 ± 3:21 min) than runners (17:30 ± 6:21 min). No differences were observed for any other measurements (P > 0.05).

Conclusion
Differences observed between combat athletes and runners suggest sport-specific metabolic adaptations. Runners had a longer time to crossover than combat athletes, indicating longer fat utilization. Future prospective studies should include a variety of exercise intensities to further elucidate differences in the transition of fuel use between sport types.
Muscle growth and anabolism in intensive care survivors (GAINS 2.0): Protocol for a multi-centre randomised; placebo controlled clinical trial of nandrolone in deconditioned adults recovering from critical illness
Authors: Anstey Matthew , Litton Ed , Habibi Maryam , Van der Lee Lisa , Palmer Robert , Tran Natalie , Mammana Bianca , Scheepers Stacey , Palermo Annamaria , Fiorilla Xavier , Mevavala Bhaumik , Regli Adrian , Jacques Angela , Wibrow Bradley 
Published: February 2025

Background
Intensive care patients can experience significant long-term impairment in mobility and function caused by their critical illness. A potential contributory factor apart from critical illness polymyoneuropathy is the low levels of anabolic hormones in these patients. Testosterone levels in critically ill patients are extremely low, even in the latter recovery phase. A potential solution to critical illness myopathy may be to provide anabolic support in addition to standard care (early physiotherapy) to further improve gains in strength.

Research question
This project aims to test whether a synthetic testosterone (nandrolone) improves muscle strength in ICU survivors compared to placebo.

Methods
GAINS 2.0 is a multicentre, randomised, double blinded placebo-controlled trial which will allocate ICU patients in a 1:1 ratio to nandrolone compared to placebo which commenced recruitment in July 2023. Adult patients admitted to the ICU, receiving nutrition for a minimum of 24 hours with an ICU stay of at least 5 days, or patients with significant weakness as result of their ICU stay (such that they are unable to mobilise independently) will be eligible to participate. Sample size will be 54 patients. Patients will be randomised to receive nandrolone 100mg (males) / 50mg(females) weekly for 3 weeks in addition to standard care. The co-primary outcomes are the time to walking with one person assisting (Intensive Care Mobility scale = 8 or more, in days from randomisation), change in muscle strength measured by the Medical Research Council (MRC) muscle strength sum score from enrolment to hospital discharge and number of days out of hospital up to day 90 post-discharge. Secondary outcomes are grip strength measured by hand-held dynamometry. SF-36 scores (quality of life and functional domains), and days to return to work, for those working pre-ICU, will be collected via a 3-month phone follow-up.

Conclusions
A previous pilot feasibility trial showed that nandrolone is safe and feasible. We hypothesize nandrolone will improve muscle strength and physical functioning at hospital discharge and at follow-up. The results of this trial may have significant interest to clinicians and patients considering the large and increasing number of patients surviving intensive care but with physical impairment. This trial may have significant implications on lowering hospital costs and daily adjusted life years.

Trial registry
anzctr.org.au; No.: ACTRN12623000729628 URL: anzctr.org.au.
Seroprevalence and associated factors for hepatitis B and hepatitis C viral infection among patients with diabetes mellitus in Northern Tanzania
Authors: Eliah Doreen T. , Chamba Nyasatu G. , Sadiq Abid M. , Mwasamwaja Amos O. , Kimondo Faustini C. , Matay Cuthbert D. , Nziku Eliada B. , Mirai Tumaini E. , Muhina Ibrahim Ali Ibrahim , Said Fuad H. , Gharib Sarah K. , Lyamuya Furaha S. , Mkwizu Elifuraha W. , Kilonzo Kajiru G. , Maro Venance P. , Shao Elichilia R. 
Published: February 2025

Background
The coexistence of viral hepatitis with diabetes mellitus (DM) significantly escalates the risk of severe outcomes. This study aimed to determine the seroprevalence and associated factors of hepatitis B (HBV) and hepatitis C (HCV) viral infections among DM patients in northern Tanzania.

Materials and methods
Conducted between February 2023 and May 2023, this hospital-based cross-sectional study enrolled 189 patients with DM from the Diabetic Clinic of Kilimanjaro Christian Medical Centre. A structured questionnaire captured relevant clinical information, and plasma blood sample was assessed for hepatitis B surface antigen and anti-hepatitis C antibody seropositivity. Data analysis employed SPSS v26, and a chi-square test was used to determine the statistical difference of HBV and HCV among patients with DM. Logistic regression was performed to determine factors associated with HBV and HCV.

Results
Among the 189 patients with DM, the seroprevalence of HBV and HCV infections stood at 2.1% and 0.5%, respectively. Males constituted a significant majority (80%) of those affected by viral hepatitis. Furthermore, 60% of affected patients were in non-union relationships (single, widowed, or divorced), and 40% reported multiple sexual partners. However, the study found no significant association between traditional associated factors and viral infection acquisition.

Conclusion
The study’s findings reveal a relatively low prevalence of HBV and HCV infections among patients with DM compared to the general population, with no significant association among factors. Nonetheless, the results underscore the importance of early screening and vaccination for HBV and HCV in patients with DM. Such efforts are crucial for curbing infection spread and reducing the risk of hepatocellular carcinoma development in this vulnerable population.
Association of the abbreviated burn severity index with mortality in severely burned patients: A meta-analysis
Authors: Deng Guangrong , Tang Ling , Yang Qian , Li Zhengyong 
Published: February 2025

Background and purpose
The ability of the abbreviated burn severity index (ABSI) to predict death among patients with severe burns remains unclear. This meta-analysis aimed to identify the association between the ABSI and mortality in severely burned patients.

Methods
The PubMed, EMBASE and Web of Science databases were searched up to September 15, 2024. The odds ratios (ORs) with 95% confidence intervals (CIs) were combined, and a subgroup analysis was conducted on the basis of age, ABSI grouping method and OR source. All the statistical analyses were performed with STATA version 15.0.

Results
Sixteen studies with 4011 cases were included in the analysis. The pooled results demonstrated that an elevated ABSI was significantly related to an increased risk of mortality (OR = 1.72, 95% CI: 1.48–2.00; P < 0.001). In addition, subgroup analysis by age (adult: OR = 1.35, P < 0.001; child: OR = 68.40, P < 0.001), ABSI grouping method (dichotomous: OR = 16.14, P < 0.001; continuous: OR = 1.59, P < 0.001) and OR source (univariate: OR = 11.42, P = 0.015; multivariate: OR = 1.51, P < 0.001) yielded similar results.

Conclusion
The ABSI serves as a reliable prognostic indicator in severely burned patients, and patients with an elevated ABSI are at increased risk of death.
Analisis Dampak ENSO terhadap Presipitasi dan Evaporasi di Selat Makassar
Authors: Alamsyah M. Zanugera , Rizqullah Fairus Jamil , Sulhana Baiq Lista Azkia , Maulana Refaldi Rizky , Cahyaningtyas Annisa Dianti , Rahmalia Fahri , Mustaqim Ikhsan , Fadhillah Shafa Nur , Baskara Ganesha Lagas , Kunarso Kunarso 
Published: February 2025

Penelitian ini menganalisis pengaruh ENSO terhadap presipitasi dan evaporasi di Selat Makassar. Data yang digunakan berupa data presipitasi dan evaporasi diperoleh dari The European Centre for Medium-Range Weather Forecast (ECMWF) serta data ONI (Oceanic Niño Index) dari National Oceanic and Atmospheric Administration (NOAA) selama 15 tahun dari 2008 - 2022. Analisis korelasi pearson digunakan untuk mengukur kekuatan dan arah hubungan linier variabel ENSO terhadap presipitasi dan evaporasi. Hasil analisis menunjukkan bahwa secara klimatologis dan spasial, presipitasi maupun evaporasi mengalami maksimum minimum yang sama. Pengaruh ENSO sendiri terhadap presipitasi dan evaporasi di Selat Makassar secara umum menunjukkan pola korelasi yang kompleks dan bervariasi sepanjang tahun, dengan perbedaan yang signifikan di tiga wilayah, yaitu Selatan, Tengah, dan Utara. Keadaan presipitasi dan evaporasi di Selat Makassar selama 15 tahun terakhir tidak secara signifikan dipengaruhi oleh El Niño dan La Niña. Hal tersebut diduga karena letak Selat Makassar sendiri berada di wilayah yang dipengaruhi oleh beberapa fenomena atmosfer global lainnya seperti munsonal, MJO, dan IOD.
Perubahan Garis Pantai Rawan Gempabumi di Lokasi Terabrasi Menggunakan Data Geofisika
Authors: Priscilla Viona Azhari , Farid Muchammad , Lidiawati Liza , Al Ansory Andre Rahmat 
Published: February 2025

Kabupaten Bengkulu Utara mempunyai garis pantai yang panjangnya sekitar 262,63 km dan berada di wilayah rawan Gempabumi yang ditunjukkan dengan besarnya nilai Peak Ground Acceleration (PGA > 300). Salah satu sumber daya pesisir di Kabupaten Bengkulu Utara yang memberikan manfaat bagi masyarakat setempat adalah kawasan di sepanjang pantai. Namun, manfaat bagi masyarakat sekitar terancam akibat fenomena abrasi di wilayah pesisir. Kabupaten Bengkulu Utara merupakan kabupaten ke-70 di indonesia yang rentan terhadap bencana abrasi pantai, sehingga masuk dalam kategori kabupaten yang sangat rentan terhadap bencana jenis tersebut. Desa Urai merupakan salah satu wilayah yang rentan terhadap abrasi pantai di Kabupaten Bengkulu Utara. Berdasarkan keadaan yang terjadi di Desa Urai, maka perlu dilakukan penelitian untuk mengetahui kecepatan perubahan garis pantai menggunakan data Citra Satelit dan data Seismik Pasif dalam bentuk kecepatan gelombang geser ( ) dengan menggunakan metode HVSR (Horizontal to Vertical Spectral Ratio). Hasil penelitian menunjukkan daerah yang mengalami abrasi memiliki kecepatan perubahan garis pantai pada rentang antara 1,41-5,04 m/tahun, dengan kecepatan gelombang geser ( ) antara 176,30-237,12 m/s. Daerah yang mengalami abrasi memiliki nilai kecepatan perubahan garis pantai yang tinggi yang didukung dengan nilai rendah yang mengindikasikan klasifikasi tanah berada pada jenis tanah lunak sampai sedang.
Parental Investments During Labor Shocks: Evidence from Vietnam’s Marine Disaster
Authors: Chan Eric W. , Nguyen Luong 
Published: February 2025

Abstract
We examine the impacts of a marine environmental disaster on parents’ educational investments in the context of Vietnam. Previous research finds that the disaster caused a labor shock in the fishing industry, a primary source of income for workers in the area studied. Using administrative data and a difference-in-differences framework, we find that parents in affected provinces decreased educational investments by 29 % during the four years after the disaster. In households with male and female children of compulsory schooling age, we estimate that affected families spent approximately 5–6 % less on educational expenditures for girls than boys in affected provinces after the disaster.
High Throughput Characterization of Mechanical Properties of Electron Beam Additive Manufactured High-Speed Steel
Authors: Kahlert M. , Hübner L. , Wegener T. , Vollmer M. , Niendorf T. 
Published: February 2025

Abstract
Additive manufacturing of metals by electron beam-based powder bed fusion (PBF-EB/M) is widely used for processing of numerous alloy systems. However, up to now there have been only a few studies investigating tool steels using PBF-EB/M. Due to high build chamber temperatures during processing, PBF-EB/M provides advantages for such steels. These conditions, for example, help to avoid cracks. In the present study, the high-speed steel (HSS) Böhler S390 Microclean (similar to M42, 1.3247) has been processed using PBF-EB/M. As a first step, two different parameter sets, i. e., two different resulting line energies and their effect on the resulting network-like cell structure, were investigated. Nanoindentation measurements revealed strong differences in hardness and elastic modulus between the network-like cell structures and the martensitic matrix, which could be attributed to the formation of carbides during solidification, which were probed by scanning electron microscopy. In addition, monotonic tensile properties were characterized on specimens built with a parameter set adapted to the geometry in order to consider one of the most critical loading scenarios for the tool steel in focus. The investigations reveal a high tensile strength with relatively low elongation at fracture.