bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiview motion tracking based on a cartesian robot to monitor Caenorhabditis elegans in standard Petri dishes
Authors: Puchalt Joan Carles , Gonzalez-Rojo Jose F. , Gómez-Escribano Ana Pilar , Vázquez-Manrique Rafael P. , Sánchez-Salmerón Antonio-José 
Published: February 2022

AbstractData from manual healthspan assays of the nematode Caenorhabditis elegans (C. elegans) can be complex to quantify. The first attempts to quantify motor performance were done manually, using the so-called thrashing or body bends assay. Some laboratories have automated these approaches using methods that help substantially to quantify these characteristic movements in small well plates. Even so, it is sometimes difficult to find differences in motor behaviour between strains, and/or between treated vs untreated worms. For this reason, we present here a new automated method that increases the resolution flexibility, in order to capture more movement details in large standard Petri dishes, in such way that those movements are less restricted. This method is based on a Cartesian robot, which enables high-resolution images capture in standard Petri dishes. Several cameras mounted strategically on the robot and working with different fields of view, capture the required C. elegans visual information. We have performed a locomotion-based healthspan experiment with several mutant strains, and we have been able to detect statistically significant differences between two strains that show very similar movement patterns.
Indolent enhancing spinal lesions mimicking spinal metastasis in pediatric patients with malignant primary brain tumors
Authors: Wu Hsin-Wei , Lin Shih-Chieh , Wu Ching-Lan , Lee Kang-Lung , Wu Chia-Hung , Chen Shu-Ting , Chen Hsin-Hung , Lee Yi-Yen , Chen Yi-Wei , Wu Chih-Chun , Hsu Ting-Rong , Chang Feng-Chi 
Published: February 2022

AbstractSpinal metastasis from malignant primary brain tumors (MPBTs) in pediatric patients is rare and often appears as enhancing lesions on MRI. However, some indolent enhancing spinal lesions (IESLs) resulting from previous treatment mimic metastasis on MRI, leading to unnecessary investigation and treatment. In 2005–2020, we retrospectively enrolled 12 pediatric/young patients with clinical impression of spinal metastasis and pathological diagnosis of their spinal lesions. Three patients had MPBT with IESL, and 9 patients had malignant tumors with metastases. The histopathologic diagnosis of IESL was unremarkable marrow change. We evaluated their MRI, CT, and bone scan findings. The following imaging findings of IESL vs. spinal metastasis were noted: (1) IESLs appeared round/ovoid (3/3, 100%), whereas spinal metastasis appeared irregular (9/9, 100%) (P = 0.005); (2) target-shaped enhancement was noted in (3/3, 100%) vs. (0/9, 0%) of cases, respectively (P = 0.005); (3) pathologic fracture of the vertebral body was noted in (1/3, 33.3%) vs. (9/9, 100%) of cases, respectively (P = 0.045); (4) expansile vertebral shape was noted in (0/3, 0%) vs. (9/9, 100%) of cases, respectively (P = 0.005); (5) obliteration of the basivertebral vein was noted in (0/3, 0%) vs. (9/9, 100%) of cases, respectively (P = 0.005); and (6) osteoblastic change on CT was noted in (3/3, 100%) vs. (2/9, 22.2%) of cases, respectively (P = 0.034). IESL in pediatric patients with MPBT can be differentiated from metastasis based on their imaging characteristics. We suggest close follow-up rather than aggressive investigation and treatment for IESL.
Evaluation of stable reference genes for qPCR normalization in circadian studies related to lung inflammation and injury in mouse model
Authors: Giri Allan , Sundar Isaac Kirubakaran 
Published: February 2022

AbstractCircadian rhythms have a profound effect on lung function and immune-inflammatory response in chronic airway diseases. Thus, understanding the molecular mechanisms of circadian gene expression of core clock-controlled genes (CCGs) may help better understand how it contributes to the physiology and pathology of lung diseases. Ongoing studies have been analyzing gene expression levels of CCGs in mouse lungs using quantitative real-time PCR (qRT-PCR). However, to date, there are no reports on the most stable reference gene in the mouse lung for circadian studies. Herein, we utilized an acute house dust mite (HDM)-sensitization mouse model to evaluate the stability of 10 reference genes commonly used for qRT-PCR normalization using 5 unique algorithms: GeNorm, NormFinder, BestKeeper, RefFinder and Qbase+. Rn18s was determined as the most stable reference gene across all samples evaluated, and Actb, the least stable reference gene. Furthermore, CircWave analysis showed no diurnal variation in the expression pattern for Rn18s but Actb showed strong diurnal changes in the lungs of both PBS (control) and HDM groups. We demonstrate systematically how using Actb as a housekeeping gene offsets the diurnal expression patterns of the CCGs and leads to statistically significant results which may not be the true reflection of the qRT-PCR analysis.
Kinematics of the viscous filament during the droplet breakup in air
Authors: Broboana Diana , Bratu Ana-Maria , Magos István , Patrascu Claudiu , Balan Corneliu 
Published: February 2022

AbstractThe dripping regime in the vicinity of the fluid droplet breakup is analyzed using the correlation between experiments and numerics. The evolutions of filament’s neck and its corresponding thinning velocity are described using the logistic functions. Three flow regions are observed as the relative time decreases: (1) a monotonous increase of the neck’s thinning velocity, where inertia and capillarity are balanced, (2) a transition domain characterized by the equilibrium between inertia, capillarity, and viscous forces, where the thinning velocity varies non-monotonically with the relative time and (3) the final droplet pinch-off, where velocity decreases or oscillates around a constant value. The distributions of the $$\upzeta$$
ζ
-coefficient (parameter related to the non-dimensional second invariant of the velocity gradient) on the filament’s surface and droplet’s profile characterize the kinematics at the interface. The regions dominated by extension, where pure elongation is located at $$\upzeta \cong 1$$

ζ
≅
1

, are determined. One main result of this study is the confirmation that distribution of the $$\upzeta$$
ζ
-coefficient is a relevant parameter to analyze and to quantify the breakup process. This result has the potential of developing novel techniques and more precise procedures in determining the interfacial rheology of viscous and complex fluids.
Need for cognition moderates the impairment of decision making caused by nightshift work in nurses
Authors: Peng Jiaxi , Lu Huijie , Zhang Jiaxi , Shao Yongcong , Wang Lei , Lv Jing 
Published: February 2022

AbstractThe current study explores the effect of nightshift work on the decision-making competence and performance of the Iowa Gambling Task (IGT) and analyzes whether individual differences in the need for cognition (NFC) can moderate this effect. A total of 107 female nurses were recruited to complete the decision-making competence scale and IGT at two times, after a night shift and after a day shift. The results revealed that the IGT scores and decision-making competence of nurses after nightshift work significantly declined, and also that the decrease in decision-making competence was related to the nurses’ performance of the IGT. Additionally, the decreasing degree of IGT and decision-making competence scores of the high-NFC group were significantly lower than those of the low-NFC group after nightshift work. In can be concluded that the decrease in decision-making competence which was related with poor decision-making due to nightshift work. NFC moderated the effect of nightshift work on decision-making.
Pathway-specific TNF-mediated metaplasticity in hippocampal area CA1
Authors: Singh Anurag , Sateesh Shruthi , Jones Owen D. , Abraham Wickliffe C. 
Published: February 2022

AbstractLong-term potentiation (LTP) is regulated in part by metaplasticity, the activity-dependent alterations in neural state that coordinate the direction, amplitude, and persistence of future synaptic plasticity. Previously, we documented a heterodendritic metaplasticity effect whereby high-frequency priming stimulation in stratum oriens (SO) of hippocampal CA1 suppressed subsequent LTP in the stratum radiatum (SR). The cytokine tumor necrosis factor (TNF) mediated this heterodendritic metaplasticity in wild-type rodents and in a mouse model of Alzheimer’s disease. Here, we investigated whether LTP at other afferent synapses to CA1 pyramidal cells were similarly affected by priming stimulation. We found that priming stimulation in SO inhibited LTP only in SR and not in a second independent pathway in SO, nor in stratum lacunosum moleculare (SLM). Synapses in SR were also more sensitive than SO or SLM to the LTP-inhibiting effects of pharmacological TNF priming. Neither form of priming was sex-specific, while the metaplasticity effects were absent in TNFR1 knock-out mice. Our findings demonstrate an unexpected pathway specificity for the heterodendritic metaplasticity in CA1. That Schaffer collateral/commissural synapses in SR are particularly susceptible to such metaplasticity may reflect an important control of information processing in this pathway in addition to its sensitivity to neuroinflammation under disease conditions.
A feasibility randomized controlled trial of a NICU rehabilitation program for very low birth weight infants
Authors: Letzkus Lisa , Conaway Mark , Miller-Davis Claiborne , Darring Jodi , Keim-Malpass Jessica , Zanelli Santina 
Published: February 2022

AbstractMotor disability is common in children born preterm. Interventions focusing on environmental enrichment and emotional connection can positively impact outcomes. The NICU-based rehabilitation (NeoRehab) program consists of evidence-based interventions provided by a parent in addition to usual care. The program combines positive sensory experiences (vocal soothing, scent exchange, comforting touch, skin-to-skin care) as well as motor training (massage and physical therapy) in a gestational age (GA) appropriate fashion. To investigate the acceptability, feasibility and fidelity of the NeoRehab program in very low birthweight (VLBW) infants. All interventions were provided by parents in addition to usual care. Infants (≤ 32 weeks' GA and/or ≤ 1500 g birthweight) were enrolled in a randomized controlled trial comparing NeoRehab to usual care (03/2019–10/2020). The a priori dosing goal was for interventions to be performed 5 days/week. The primary outcomes were the acceptability, feasibility and fidelity of the NeoRehab program. 36 participants were randomized to the intervention group and 34 allocated to usual care. The recruitment rate was 71% and retention rate 98%. None of the interventions met the 5 days per week pre-established goal. 97% of participants documented performing a combination of interventions at least 3 times per week. The NeoRehab program was well received and acceptable to parents of VLBW infants. Programs that place a high demand on parents (5 days per week) are not feasible and goals of intervention at least 3 times per week appear to be feasible in the context of the United States. Parent-provided motor interventions were most challenging to parents and alternative strategies should be considered in future studies. Further studies are needed to evaluate the relationship between intervention dosing on long term motor outcomes.
Citrate pharmacokinetics in critically ill liver failure patients receiving CRRT
Authors: Thanapongsatorn Peerapat , Chaijamorn Weerachai , Sirivongrangson Phatadon , Tachaboon Sasipha , Peerapornratana Sadudee , Lumlertgul Nuttha , Lucksiri Aroonrut , Srisawat Nattachai 
Published: February 2022

AbstractCitrate has been proposed as anticoagulation of choice in continuous renal replacement therapy (CRRT). However, little is known about the pharmacokinetics (PK) and metabolism of citrate in liver failure patients who require CRRT with regional citrate anticoagulation (RCA). This prospective clinical PK study was conducted at King Chulalongkorn Memorial Hospital between July 2019 to April 2021, evaluating seven acute liver failure (ALF) and seven acute-on-chronic liver failure (ACLF) patients who received CRRT support utilizing RCA as an anticoagulant at a citrate dose of 3 mmol/L. For evaluation of the citrate PK, we delivered citrate for 120 min and then stopped for a further 120 min. Total body clearance of citrate was 152.5 ± 50.9 and 195.6 ± 174.3 mL/min in ALF and ACLF, respectively. The ionized calcium, ionized magnesium, and pH slightly decreased after starting citrate infusion and gradually increased to baseline after stopping citrate infusion. Two of the ACLF patients displayed citrate toxicity during citrate infusion, while, no ALF patient had citrate toxicity. In summary, citrate clearance was significantly decreased in critically ill ALF and ACLF patients receiving CRRT. Citrate use as an anticoagulation in these patients is of concern for the risk of citrate toxicity.
Deep learning-based segmentation of the thorax in mouse micro-CT scans
Authors: Malimban Justin , Lathouwers Danny , Qian Haibin , Verhaegen Frank , Wiedemann Julia , Brandenburg Sytze , Staring Marius 
Published: February 2022

AbstractFor image-guided small animal irradiations, the whole workflow of imaging, organ contouring, irradiation planning, and delivery is typically performed in a single session requiring continuous administration of anaesthetic agents. Automating contouring leads to a faster workflow, which limits exposure to anaesthesia and thereby, reducing its impact on experimental results and on animal wellbeing. Here, we trained the 2D and 3D U-Net architectures of no-new-Net (nnU-Net) for autocontouring of the thorax in mouse micro-CT images. We trained the models only on native CTs and evaluated their performance using an independent testing dataset (i.e., native CTs not included in the training and validation). Unlike previous studies, we also tested the model performance on an external dataset (i.e., contrast-enhanced CTs) to see how well they predict on CTs completely different from what they were trained on. We also assessed the interobserver variability using the generalized conformity index ($$\hbox {CI}_{\mathrm{gen}}$$

CI
gen

) among three observers, providing a stronger human baseline for evaluating automated contours than previous studies. Lastly, we showed the benefit on the contouring time compared to manual contouring. The results show that 3D models of nnU-Net achieve superior segmentation accuracy and are more robust to unseen data than 2D models. For all target organs, the mean surface distance (MSD) and the Hausdorff distance (95p HD) of the best performing model for this task (nnU-Net 3d_fullres) are within 0.16 mm and 0.60 mm, respectively. These values are below the minimum required contouring accuracy of 1 mm for small animal irradiations, and improve significantly upon state-of-the-art 2D U-Net-based AIMOS method. Moreover, the conformity indices of the 3d_fullres model also compare favourably to the interobserver variability for all target organs, whereas the 2D models perform poorly in this regard. Importantly, the 3d_fullres model offers 98% reduction in contouring time.
Influence of increasing SnO2 content on the mechanical, optical, and gamma-ray shielding characteristics of a lithium zinc borate glass system
Authors: Sayyed M. I. , Almuqrin Aljawhara H. , Mahmoud K. A. , Abouhaswa A. S. 
Published: February 2022

AbstractA series of six samples were prepared based on the chemical composition of 65B2O3 + 20ZnO + (15-x)LiF + xSnO2 (where x = 0, 0.25, 0.5, 0.75, 1, and 1.25 mol%) to study the role of SnO2 on enhancing the optical and radiation attenuation capacity of the prepared glasses. The preparation of the glass series was performed using the melt quenching method at 1100 °C for 60 min. The density of the fabricated samples was measured using an MH-300A densimeter. The optical parameters of the fabricated glasses were calculated based on the spectrum recorded by a Cary 5000 UV–Vis–NIR double beam spectrophotometer in a wavelength range of 200 to 3000 nm. Furthermore, Monte Carlo simulation code and the XCOM online database were used to estimate the gamma-ray shielding capacity of the fabricated samples from 0.244 to 2.506 MeV. The results show enhanced gamma-ray shielding capacity due to the replacement of LiF by SnO2. The linear attenuation coefficient at 0.244 MeV was enhanced from 0.352 to 0.389 cm−1. The half-value thickness of the investigated glasses decreased from 1.967 to 1.784 cm when the increasing addition of SnO2 from 0 to 1.25 mol%.
Spatial patterns of urbanising landscapes in the North Indian Punjab show features predicted by fractal theory
Authors: Nguyen Thanh Thi , Hoffmann Ellen , Buerkert Andreas 
Published: February 2022

AbstractUnderstanding and governing human settlement patterns is a major challenge of the urban age. While rural settlements emerge as parts of agricultural landscapes, cities typically evolve in economically strategic locations, and over time form hierarchical systems of cities. Purposeful planning and the collective, self-organized behavior of the inhabitants interact in the development of regional settlement patterns. Since self-organizing systems often produce fractal patterns in nature, this study combines approaches of land use science, city ranking, and urban planning under a fractal theory framework, to analyze the settlement system of the Indian Punjab. Scaling levels were defined by discontinuities in the size distribution of built-up areas (Global Urban Footprint), which correlated to population-based classifications (r = 0.9591). Self-similarity across scales was supported by geo-statistical similarity (p < 0.05) of distances and angles between settlements of successive classes, and the overall fractal dimension of DB = 1.95. When compared to a modeled Sierpinski Carpet, more than 50% of the settlements met the fractal geometry rules at larger scales. The spatial distribution of small villages, however, deviated, indicating a scale-related shift in organizing principles. Explicitly acknowledging cross-scale relations and self-organisation in regional planning policies may lead to more sustainable settlement structures that are in harmony with natural system properties.
Unique current connecting Southern and Indian Oceans identified from radium distributions
Authors: Inoue Mutsuo , Hanaki Shotaro , Kameyama Hiroaki , Kumamoto Yuichiro , Nagao Seiya 
Published: February 2022

AbstractWe examined the spatial variations in226Ra and228Ra (activities) concentrations from the surface to a depth of 830 m in the Indian and Southern Oceans from December 2019 to January 2020.226Ra concentrations at the surface increased sharply from 30° S to 60° S along a ~ 55° E transect (1.4–2.9 mBq/L), exhibiting small vertical variations, while228Ra decreased southward and became depleted in the Southern Ocean. These distributions indicated the ocean-scale northward lateral transport of226Ra-rich and228Ra-depleted currents originating from the Antarctic Circumpolar Current (ACC).226Ra concentrations indicated that the fractions of the ACC at depths of 0–800 m decreased from 0.95 to 0.14 between 60° S and 30° S. The ACC fractions in the subantarctic western Indian Ocean were higher than those previously reported in the eastern Indian region, indicating preferential transport of the ACC. The fractions obtained were approximately equivalent to those in the western Indian Ocean in the 1970s. This could be attributed to the minimal southward shift of the polar front due to global warming over the last 50 years.
Analysis of colonic mucosa-associated microbiota using endoscopically collected lavage
Authors: Miyauchi Eiji , Taida Takashi , Kawasumi Masami , Ohkusa Toshifumi , Sato Nobuhiro , Ohno Hiroshi 
Published: February 2022

AbstractThe bacterial composition of the gut lumen and mucosa is distinct and the mucosa-associated bacteria are thought to play a more critical role in interactions with the host immune system. However, limited studies of the gut mucosal microbiota in humans have been available due to methodological challenges. Here, we evaluated the potential use of colonic lavage samples for mucosal microbiota analysis in humans. Among the different types of colonic mucosal samples collected from healthy volunteers, the lavage samples contained a higher amount of bacterial DNA and were less contaminated with host DNA compared to mucosal brushing (brush) and biopsy. Although 16S gene amplicon sequencing showed that the bacterial composition of the lavage was intermediate between that of feces and biopsy, mucosal bacteria abundant in the biopsy were also enriched in lavage samples. Furthermore, differences in mucosal microbes between non-smokers and smokers were detectable in lavage samples. Our data emphasize that colonic lavage is suitable for analysis of the mucosal microbiota. Given its minimal invasiveness and high bacterial DNA content, the colonic lavage will promote research on the human mucosal microbiota, especially in gastrointestinal disorders.
Mnemonic-opto-synaptic transistor for in-sensor vision system
Authors: Han Joon-Kyu , Chung Young-Woo , Sim Jaeho , Yu Ji-Man , Lee Geon-Beom , Kim Sang-Hyeon , Choi Yang-Kyu 
Published: February 2022

AbstractA mnemonic-opto-synaptic transistor (MOST) that has triple functions is demonstrated for an in-sensor vision system. It memorizes a photoresponsivity that corresponds to a synaptic weight as a memory cell, senses light as a photodetector, and performs weight updates as a synapse for machine vision with an artificial neural network (ANN). Herein the memory function added to a previous photodetecting device combined with a photodetector and a synapse provides a technical breakthrough for realizing in-sensor processing that is able to perform image sensing and signal processing in a sensor. A charge trap layer (CTL) was intercalated to gate dielectrics of a vertical pillar-shaped transistor for the memory function. Weight memorized in the CTL makes photoresponsivity tunable for real-time multiplication of the image with a memorized photoresponsivity matrix. Therefore, these multi-faceted features can allow in-sensor processing without external memory for the in-sensor vision system. In particular, the in-sensor vision system can enhance speed and energy efficiency compared to a conventional vision system due to the simultaneous preprocessing of massive data at sensor nodes prior to ANN nodes. Recognition of a simple pattern was demonstrated with full sets of the fabricated MOSTs. Furthermore, recognition of complex hand-written digits in the MNIST database was also demonstrated with software simulations.