bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism and Prevention Technologies of Reservoir Gas Disaster in Abandoned Oil Well of Coal Mine
Authors: Shao Yawu , Suo Yonglu , Xiao Jiang , Li Qiannan , Yang Tao 
Published: December 2022

Abstract
The reservoir gas disaster has distinct characteristics and is a key factor that threatens the safe and green mining of coal mines in the costorage area of coal and petroleum resources. In order to solve the problem of prevention and control of reservoir gas disasters in coal mines, the characteristics of oil-bed gas disasters in abandoned oil wells in coal mines were analyzed, and the oil-bed gas disaster mechanism of abandoned oil wells without isolation coal pillars was revealed to study the scope of gas disasters around oil wells under the influence of production. The research shows that: (1) abandoned oil well reservoir gas disasters have the characteristics of high gas pressure, high concentration, large lateral influence area, wide vertical sweep range, and frequent disasters, which seriously threaten the safety and green mining of coal mines; (2) divide the reservoir gas disaster of abandoned oil wells into the high-pressure gas disaster in the well and the disaster in the surrounding oil-bed gas enrichment area; (3) according to the numerical simulation results that the maximum damage depth of the coal seam mining floor is 38.6 m and the seepage height of high-pressure oil-bed gas is 40 m, the safety factor k is introduced, and the reservoir gas sweeping range of the abandoned oil well is determined to be 95.4 m below the coal seam to the surface; (4) the comprehensive prevention and control technical scheme of oil-bed gas for controlling high-pressure oil-bed gas in wells by ground plugging and downhole injection and injection of diluent to control enriched areas was proposed, which successfully solved the problem of safe and efficient exploitation of Shuangma coal mine in Ningdong coalfield by abandoned oil wells. The research results provide effective solutions for the realization of green mining in many coal mines in the costorage area of coal and oil resources in China and have important application value for the prevention and control of dynamic disasters in the costorage area of resources.
Comprehensive analysis of HDAC7 expression and its prognostic value in diffuse large B cell lymphoma
Authors: Gao Tianqi , Lu Weiguo , Zhuang Guangyan , Li Yongcong , Zeng Qingli , Li Xiaozhen , Xiao Mingfeng 
Published: December 2022

Abstract
Background: HDAC7 is a class IIa histone deacetylase (HDAC) that exhibits lymph-specific expression patterns in the hematopoietic system. HDAC7 loss or dysregulation may lead to B cell-based hematological malignancies. This study aimed to explore the prognostic value of HDAC7 in patients with diffuse large B cell lymphoma (DLBCL).
Methods: RNA sequencing data and clinical information for HDAC7 in DLBCL were collected from The Cancer Genome Atlas (TCGA) database and analyzed using R software. Paired t and Mann–Whitney U tests were used to detect differences between DLBCL and adjacent normal tissues, and the pROC software package was used to generate receiver operator characteristic (ROC) curves to detect cut-off values for HDAC7. Data from paraffin-embedded specimens from the two groups were used for validation of external immunohistochemical (IHC) staining. The relationship between DLBCL and HDAC7 was explored by enrichment analysis using the Gene Oncology and Kyoto Encyclopedia of Genes and Genomesdatabases. The tumor immunity estimation resource (TIMER) and integrated repository portal for tumor-immune system interactions (TISIDB) databases were used to analyze the correlation between HDAC7 and DLBCL immune cell infiltration. Survival analysis of HDAC7 in patients with DLBCL was performed using the PrognoScan database.
Results: Compared with that in normal tissues, HDAC7 mRNA was overexpressed in DLBCL. The HDAC7 IHC scores of stage III and IV DLBCL patients were significantly lower than those of stageI and II DLBCL patients, which was associated with shorter overall survival (OS) and disease-specific survival (DSS). In addition, the higher expression of HDAC7 may play a role in the lower level of immune infiltration in DLBCL.
Conclusions: Downregulation of HDAC7 expression was correlated with poor prognosis and immune infiltration in DLBCL patients. This has potential as a novel marker for the prognosis of DLBCL patients.
Human Decellularized Endometrial Scaffold Seeded by Myometrial Smooth Muscle Cells Supports the Differentiation of Human Endometrial Mesenchymal Cells to Epithelial and Stromal Cells ​
Authors: Sargazi Zinat , Zavareh Saeed , Salehnia Mojdeh 
Published: December 2022

Abstract
Background: This study designed to evaluate the co-culturing of human mesenchymal endometrial cells (EMCs) and myometrial smooth muscle cells (MSMCs) in decellularized scaffold as a natural bioscaffold to formation of the endometrial-like structure.
Methods: After decellularization of the human endometrium, cell seeding was performed by centrifugation of human EMCs with different speeds and times in 15 experimental subgroups. Analysis of residual cell count in suspension was done in all subgroups and the method with the lower amount of suspended cells was selected for subsequent study. Then, the human EMCs and the MSMCs were seeded on the decellularized tissue and cultured for one week then, differentiation of the seeded cells was assessed by morphological and gene expression analysis.
Results: The cell seeding method by centrifuging at 7000 rpm for 2 minutes showed the highest number of seeded cells and the lowest number of residual cells in suspension. The endometrial-like structure formed and the epithelial-like cells had some protrusions on their surface and the stromal cells had spindle and polyhedral morphology. The MSMCs almost were homed at the periphery of mesenchymal cells similar to their arrangement in the native uterus. The expression of endometrial-related genes (SPP1, MMP2, ZO-1, LAMA2 and COL4A1) confirmed these observations. The low level of expression of the OCT4 gene as a pluripotency marker in seeded cells confirmed the differentiation of these cells.
Conclusion: This study showed that the co-culturing of human EMCs with the MSMCs on the decellularized scaffold could improve the formation of endometrial-like structures.
Long-term results of 107 cases undergoing posterior approach for transpedicular corpectomy with instrumentation and circumferential arthrodesis from a single-center
Authors: YILMAZ Murat , Ikizoglu Ersin , ARSLAN Mert , ERTEM Onder , ERBAYRAKTAR Serhat , YUCESOY Kemal 
Published: December 2022

Abstract
Study design: Retrospective cohort study.
Objectives: We aimed to evaluate the less morbid and efficacy of posterior transpedicular corpectomy and circumferential instrumentation using a cage or bone graft for thoracolumbar corpectomy.
Methods: This study was performed using the medical records of 107 consecutive patients treated surgically between June 1999 and July 2021. The patients suffering from spinal cord compression due to thoracolumbar vertebral pathologies as trauma, tumor, and osteomyelitis that involved T5 to L5 segments underwent posterolateral transpedicular corpectomy with or without sagittal balance reconstruction. Circumferential reconstruction was accomplished using either mesh cages or strut bone graft along with posterior instrumentation and fusion. The demographic data, clinical presentation, neuroradiological findings, surgical treatment methods, and therapeutic outcomes were analyzed.
Results: The average age was 51.03 (range: 17 to 76) years. The pathological fractures were due to primary or metastatic bone tumors in 80 (74.77%) cases, granulomatous infections in 16 (14.95%) cases, and trauma in 11 (10.28%) patients. There were no perioperative mortalities and the mean follow-up period was 48 months (range: 14 to 166 months). Therapeutic outcomes revealed satisfactory clinical recovery in all patients.
Conclusion: Transpedicular posterior corpectomy and circumferential construction is a proper, effective, and reliable surgical technique for a wide spectrum of thoracolumbar vertebral pathologies. Attributed to lower complication rates and shorter length of hospital stay compared to the anterior route, it must be considered in selected cases as an alternative to the difficult anterior approach.
Obesity in Refugees Post-resettlement in a High-income Country: A Meta-Analysis
Authors: Kibibi Niclette , Dena Isabelle , Cummings Precious de-Winton , Hicks Chelsea D. , Bao Wei , Schweizer Marin L. 
Published: December 2022

Abstract

Background
Resettled refugees have a lower risk of obesity at arrival but over time this risk increases. We systematically investigated the association between refugee status and obesity after resettlement in a high-income nation.
Methods
We searched PubMed, Embase, OpenGrey and bibliographies of retrieved articles, with no date, location, and language restrictions, for observational studies assessing obesity rates in resettled refugees compared to the host population.
Results
Nine studies were analyzed. We found no evidence of increased risk of obesity among refugees compared to the host population, with significant heterogeneity across studies. Only sub-analysis by sex showed a significantly increased risk of obesity among female refugees compared to the host population.
Discussion
Pooled analyses showed no increased risk of obesity among refugees in various host nations. However, the magnitude of the association between refugee status and obesity suggests a protective effect that should be explored further.
Reticulinasus salahi (Acarina: Argasidae), an ectoparasite of bats and man in the Palaearctic and Afrotropics: an annotated review of records with the first pathogens detected
Authors: Ševčík Martin , Špitalská Eva , Kabát Peter , Lučan Radek K. , Maliterná Michaela , Reiter Antonín , Uhrin Marcel , Benda Petr 
Published: December 2022

Abstract

The soft ticks of the genusReticulinasusSchulze, 1941 (family Argasidae Koch, 1844) are ectoparasites of various bat species of the Afrotropic, Australasian, and Oriental regions.Reticulinasus salahi(Hoogstraal, 1953) is the only representative of the genus that occurs in the western part of the Palaearctic. This unusual distribution reflects the home range of its primary host,Rousettus aegyptiacus(Geoffroy, 1810) from the family Pteropodidae. However, the complete summary of available records and a review of the host species, including re-determination of the originally reported hosts and new records, prove that this parasite occurs also in areas exceeding the distribution range ofR.aegyptiacus. Besides the primary host, the occurrence was confirmed also on bats of the families Vespertilionidae and Emballonuridae,Otonycteris hemprichiiPeters, 1859 andTaphozous perforatusGeoffroy, 1818. SinceO. hemprichiiandR. aegyptiacusforage in different habitats and also their natural roosts differ, the parasite transfer between them probably happened in a common roost in anthropogenous conditions. On the other hand, inT. perforatusthe transfer from the primary host species could be expected in a common underground roost either in natural or artificial conditions. Some of the tick specimens collected fromR. aegyptiacusbetween July 2010 and March 2012 in Jordan and Oman, were tested for pathogen presence. The MHV-68 virus and the bacteriaBorellia burgdorferiis.l. were found in the ticks from both countries. In the ticks from Jordan, the presence ofRickettsia slovaca-likewas confirmed, and CandidatusEhrlichia shimanensisin the larva from Oman. The anthropogenous occurrence ofR.salahicombined with the existence of its pathogens represents a potential health issue for human visitors of the bat roosts within the distribution range ofR.aegyptiacus.
Greenness assessment of developed derivative spectrophotometric method for simultaneous determination of ascorbic acid and folic acid in pharmaceutical formulation
Authors: Aziz Kayan S. , Qader Hemn A. 
Published: December 2022

Abstract

A simple, precise, green, and white analytical procedure have been developed for the simultaneous estimation of ascorbic acid and folic acid with the aid of the zero-crossing first derivative spectrophotometric technique. The standard solutions of ascorbic acid and folic acid were prepared in phosphate buffer (pH = 7). At the zero crossing points of folic acid (249.6 and 281) nm, ascorbic acid showed a measurable amplitude of the peak-to-baseline and linear over the concentration ranges of (0.17–12.0) µg/mL and (0.37–12) µg/mL with detection limits of 0.057 µg/mL and 0.121 µg/mL, respectively, while folic acid displayed an observable amplitude of the peak-to-baseline method at the zero-crossing point of ascorbic acid (265.6 nm), with (0.26–15.0) µg/mL and a detection limit of 0.088 µg/mL. The greenness of the proposed method was assessed based on white analytical chemistry (WAC), analytical greenness (AGREE), and green analytical procedure index (GAPI). The method was successfully applied for the simultaneous determination of ascorbic acid and folic acid in pharmaceutical formulation and synthetic laboratory mixtures with reasonable precision, accuracy, and recovery.
Assessing environmental and spatial drivers of non-marine ostracod metacommunities structure in Northern and Southern Patagonian environments
Authors: Coviaga Corina , Pérez A. Patricia , Ramos Lorena Y. , Zalazar Laura , García Patricia E. , Cusminsky Gabriela C. 
Published: December 2022

Abstract

Understanding the mechanisms structuring the metacommunities is a fundamental question in community ecology. Ostracods are an ideal study group for metacommunity studies, due to their environmental sensitivity, high dispersal capacity and increased use as biological proxy in (paleo-) ecology studies. We studied the contribution of environmental and spatial processes in structuring non-marine ostracod metacommunities in Northern and Southern Patagonia. A total of 37 ostracod species were found at the 105 environments studied. According to variation partitioning results, in Northern Patagonia the metacommunities were influenced by a combination of species sorting (environmental control, including non-spatial and spatial components) and spatial effects (e.g. dispersal limitation). While species sorting (environmental control, due to non-spatial components) was the principal mechanism structuring Southern Patagonia. Discrepancies between the degree of environmental and spatial control structuring ostracod metacommunities could be associated to differences in study areas extension and in sampled sites distances, more than differences in Ostracoda response. Instead, the spatial extent not influenced total beta diversity and their components between the studied areas. Our study expanded the understanding of the drivers affecting the non-marine ostracod metacommunities in Patagonia, Argentina. And also, about the variables structuring the ostracod assemblages. Increase the knowledge in these aspects have important implications for (paleo-) ecology studies, allowing trustworthy interpretations in biological assessment programs and paleoenvironmental interpretations.
The complex etiology of Epilepsy: Genetic analysis and HLA association in patients in the Middle East
Authors: Fadda Abeer , Alsabbagh Mohamed , Vasudeva Dhanya , Saeed Amira , Aglan Sara , Hubrack Satanay Z , Benini Ruba , Lo Bernice , Zamel Khaled 
Published: December 2022

Abstract

Epilepsy is one of the most common neurological disorders. The cost to the health system and the impact on quality of life for patients with intractable epilepsies and associated comorbidities is significant. Disease etiology and pathogenesis are still not well understood. Genetic mutations have been shown to be associated with 70% of epilepsies, with the majority being non-monogenic, and the remaining 30% enigmatic. This knowledge gap necessitates further research. The goal of this study is to partially bridge this gap through the genetic analysis of a cohort of patients with epilepsy from an understudied and highly consanguineous population, primarily of ethnicities from the Middle East and North Africa region. Whole exome sequencing was carried out in 81 patients with epilepsy and their family members at a tertiary center in Qatar. We used the data to identify pathogenic variants and type HLA alleles for 13 class I & II genes. We associated the resulting alleles with disease status, using controls of a closely related ethnicity. The genetic yield was approximately 22% for known epilepsy genes. We also suggest a list of 20 genes that could be culprits. Analysis of the biological pathways in which these genes are involved show that focal and generalized epilepsy genes are highly interwound. HLA analysis revealed that class II HLA genes are associated with disease status, particularly DRB4*03:01N, which plays a strong protective role. Our findings suggest that an immune etiology may contribute to the disease together with a genetic culprit, emphasizing the complexity of the etiology of the disease.
The Influence of Probe Curvature on Articular Cartilage Friction Measurements
Authors: Guo Emily , Henry Kaylin , Himmelmann Hannah K. , Chu Nolan , Hanson R. Reid , Schulze Kyle , Jackson Robert 
Published: December 2022

Abstract
The potential lubrication mechanisms for articular cartilage remain as complex as the material itself. Many studies have examined systematically the effects of sliding velocity on the frictional response of cartilage but a systematic analysis of contact area remains largely uncharted. Here we explore this potential effect three ways: a constant force experiment, a constant pressure experiment, and a non-constant pressure and force experiment. Hard, impermeable spheres are used as probes and with sizes varied between 1.6 mm and 25.4 mm allowing a sweep of physiologically relevant pressures. The aim is to effectively isolate the influence of the probe curvature on the friction measurements. Articular cartilage samples were collected from horses at necropsy from the left and right distal, medial aspect of the radius. Each test was run for five minutes at a reciprocated sliding speed of 1 mm/s. Two lubrication regimes are observed during testing, an area-dependent regime at lower areas and an area-independent regime at higher areas. Therefore measurements made using different probe sizes will most likely result in different measurements of the friction behavior and great care should be given when comparing these results.
Evaluating the Long- and Short Run Asymmetrical Effects of Climate Change on Crop Production: Empirical Evidence from Pakistan
Authors: Shar Rashid Usman , Qi Yin , Bughio Amanuallah , Jamali Riaz Hussain , Arthur Anita Afra 
Published: December 2022

Abstract

This study examines the long- and short-run asymmetrical effects of climate change on crop production by analyzing an annual time series from 1990 to 2019. In order to model the long-run and short-run cointegrations of a number of variables across time, as well as their causality directions, an ARDL (Autoregressive Distributed Lag) method was employed. The results of the study were also analyzed using a regression model in order to assess the reliability of the findings. As a preliminary result, a causal relationship between the variables, on a long-term basis, has been established using the Fully Modified Ordinary Least Squares (FMOLS), Canonical Cointegration Regression (CCR), and Vector Error Correction Model (VECM). The study found that crop production and carbon dioxide emissions in the country have a very strong relationship both on a short-term as well as a long-term basis, as well as showing a positive relationship between labor force, fertilizer use, and water availability over the long-term. A long-term analysis of the labor force, fertilizer use, and water availability shows a significant positive correlation between the three variables. As a result of the short-run study, several factors were positively and statistically significant, including carbon dioxide emissions, temperature, labor force, and fertilizer use. There was a significant influence of both climatic and non-climatic factors on Pakistan's crop production based on a paired Granger causality test. Based on the study's conclusions, Pakistan's policymakers must develop agricultural policies, strategies, and plans for the purpose of reducing carbon dioxide emissions, according to the conclusion of the study. Thus, by encouraging environmentally friendly farming methods, the country will be able to increase its efforts toward achieve sustainable agriculture. It is important for the country to promote environmentally friendly farming in order to achieve a sustainable agriculture industry.
Numerical Estimation Method for the Generalized Weibull distribution Parameters
Authors: Maswadah M. 
Published: December 2022

Abstract

In this study, a new estimation method using the Runge-Kutta iteration technique is presented to improve the maximum likelihood estimation method. The improved method has been applied to the generalized Weibull distribution, which is a member of a family of distributions (T-X family). The estimates of the generalized Weibull model parameters were derived using the Runge-Kutta, maximum likelihood, and Bayesian methods based on the generalized progressive hybrid censoring scheme, via a Monte Carlo simulation. The Simulation results indicated that the Runge-Kutta estimation method is highly efficient and outperforms the maximum likelihood estimation and Bayesian estimation methods based on the informative and kernel priors. Finally, two real data sets were studied to ensure the Runge-Kutta estimation method can be used very effectively than the most popular estimation methods in fitting and analyzing real lifetime data.
Analysis of the changes in intestinal lipid absorption after small bowel resection in mice
Authors: Sasaki Takato , Masumoto Kouji , Ono Kentarou , Goto Yudai , Nakagawa Yoshimi , Shimano Hitoshi 
Published: December 2022

Abstract

Purpose
Prolonged lipid malabsorption occurs after small bowel resection (SBR). We aimed to clarify the underlying mechanism using a mouse model.
Methods
SBR mice were created by resecting 50% of the proximal intestine. In sham mice, the intestine was transected and re-anastomosed. Intravenous nutrition was not initiated and mice were fed a liquid elemental diet followed by a solid standard rodent diet for one week each. Animals were sacrificed on postoperative day (POD)7 or POD14, and the following data were analyzed: body weight, caloric intake, histopathological findings, and the mRNA expression of genes related to intestinal lipid absorption in the remnant intestines (jejunum and ileum).
Results
SBR group showed significantly delayed body weight recovery, despite comparable caloric intake. A histopathological examination revealed increased villus height and deepening crypts in the remnant jejunum and ileum as “morphological adaptation” after SBR. Importantly, huge differences in the mRNA expression of genes related to intestinal lipid absorption were observed between the remnant jejunum and ileum, and above differences were only slight changed after SBR on both POD7 and POD14.
Conclusions
Insufficient compensation of the gene expression related to intestinal lipid absorption might be the cause of prolonged lipid malabsorption after SBR.