bluuebunny/crossref_metadata_2025_split huggingface dataset:
An environmentally responsive transcriptional state modulates cell identities during root development
Authors: Oliva Marina , Stuart Tim , Tang Dave , Pflueger Jahnvi , Poppe Daniel , Jabbari Jafar S. , Gigante Scott , Dragwidge Jonathan Michael , Whelan James , Lewsey Mathew G. , Lister Ryan 
Published: March 2022

AbstractRoots are fundamental organs for plant development and response to their environment: they anchor the plant to its growth substrate, uptake nutrients and water vital to plant growth, and can sense and respond to a variety of biotic and abiotic stresses. The architecture of root systems and their growth are known to be strongly affected by the environmental conditions found in the soil. However, the acquisition of cell identities at the root meristem is still mainly viewed as ontogenetically driven, where a small number of stem cells generate all the cell types through stereotyped divisions followed by differentiation, along a simple developmental trajectory. The extent to which environmental cues precisely shape and affect these developmental trajectories remains an open question. We used single-cell RNA-seq, combined with spatial mapping, to deeply explore the trajectories of cell states at the tip of Arabidopsis roots, known to contain multiple developing lineages. Surprisingly, we found that most lineage trajectories exhibit a stereotyped bifid topology with two developmental trajectories rather than one. The formation of one of the trajectories is driven by a strong and specific activation of genes involved in the responses to various environmental stimuli, that affects only of a subset of the cells in multiple cell types simultaneously, revealing another layer of patterning of cell identities in the root that is independent of cell ontogeny. We demonstrate the robustness of this environmentally responsive transcriptional state by showing that it is present in a mutant where cell type identities are greatly perturbed, as well as in different Arabidopsis ecotypes. We also show that the root can adapt the proportion of cells that acquire this particular state in response to environmental signals such as nutrient availability. The discovery of this cell state reveals new layers of cell identity that may underpin the adaptive potential of plant development.
Lyophilization induces alterations in cryptococcal exopolysaccharide resulting in reduced antibody binding
Authors: Wear Maggie P. , Hargett Audra A. , Kelly John E. , McConnell Scott A. , Crawford Conor J. , Freedberg Darón I. , Stark Ruth E. , Casadevall Arturo 
Published: March 2022

AbstractThe structural, antigenic, and immunological characterization of microbial polysaccharides requires purification that often involves detergent precipitation and lyophilization. Here we examine physicochemical changes induced by lyophilization on exopolysaccharide (EPS) of the pathogenic fungus Cryptococcus neoformans. Solution 1H NMR reveals significant anomeric signal attenuation following lyophilization of native EPS while 1H ssNMR shows few changes, suggesting diminished molecular motion and consequent broadening of 1H NMR polysaccharide resonances. 13C ssNMR, dynamic light scattering, and transmission electron microscopy show that, while native EPS has rigid molecular characteristics and contains small, loosely packed polysaccharide assemblies, lyophilized and resuspended EPS is disordered and contains larger dense rosette-like aggregates, suggesting that structural water molecules in the interior of the polysaccharide assemblies are removed during extensive lyophilization. Importantly, mAbs to C. neoformans polysaccharide binds the native EPS more strongly than lyophilized EPS. Together, these observations argue for caution when interpreting the biological and immunological attributes of polysaccharides that have been lyophilized to dryness.
Conserved Pib2 regions have distinct roles in TORC1 regulation at the vacuole
Authors: Troutman Kayla K. , Varlakhanova Natalia V. , Tornabene Bryan A. , Ramachandran Rajesh , Ford Marijn G.J. 
Published: March 2022

AbstractTORC1 is a critical controller of cell growth in eukaryotes. In yeast, the presence of nutrients is signaled to TORC1 by several upstream regulatory sensors that together coordinate TORC1 activity. TORC1 localizes to both vacuolar and endosomal membranes, where differential signaling occurs. This localization is mimicked by Pib2, a key upstream TORC1 regulator that is essential for TORC1 reactivation after nutrient starvation or pharmacological inhibition. Pib2 has both positive and negative effects on TORC1 activity, but the mechanisms remain poorly understood. Here, we pinpoint the Pib2 inhibitory function on TORC1 to residues within short, conserved N-terminal regions. We also show that Pib2 C-terminal regions, helical region E and tail, are essential for TORC1 reactivation. Further, the Pib2 FYVE domain is essential for vacuolar localization, however, it is surprisingly unnecessary for recovery from rapamycin exposure. Using chimeric Pib2 targeting constructs, we show that endosomal localization is not necessary for TORC1 reactivation and cell growth after rapamycin treatment. Thus, a comprehensive molecular dissection of Pib2 demonstrates that each of its conserved regions differentially contribute to Pib2 regulation of TORC1 activity.
Lung injury epigenetically primes mesenchyme for amplified activation upon re-injury
Authors: Jones Dakota L. , Caporarello Nunzia , Meridew Jeffrey A. , Choi Kyoung M. , Haak Andrew J. , Link Patrick A. , Tan Qi , Ordog Tamas , Ligresti Giovanni , Tschumperlin Daniel J. 
Published: March 2022

AbstractThe lungs have a remarkable capacity to repair. However, repetitive injury can lead to progressive fibrosis and end-stage organ failure. Whether tissue-resident mesenchymal cell populations retain epigenetic memory of prior injuries that contribute to this pathological process is unknown. Here we used a genetic lineage labeling approach to mark the lung mesenchyme prior to injury, then performed multi-modal analyses on isolated lung mesenchyme during the initiation, progression and resolution of the fibrotic response. Our results demonstrate the remarkable epigenetic and transcriptional plasticity of the lung mesenchyme during fibrogenic activation and de-activation. Despite this plasticity, we also find that the lung mesenchyme retains specific epigenetic traits (memory) of prior activation, resulting in amplified induction of a fibrogenic program upon re-injury. We identify Runx1 as a critical driver of both fibrogenic activation and epigenetic memory. Comparison of fresh isolated and cultured lung mesenchyme demonstrates that Runx1 is spontaneously activated in standard culture conditions, previously masking these roles of Runx1. Genetic and pharmacological targeting of Runx1 dampens fibrogenic mesenchymal cell activation in cell and tissue models, confirming its functional importance. Finally, publicly available scRNAseq data reveal selective expression of Runx1 in the fibrogenic cell subpopulations that emerge in mouse and human fibrotic lung tissue. Collectively, our findings implicate Runx1 in both the initiation and memory of fibrogenic mesenchymal cell activation that together prime amplified mesenchymal cell responses upon repeated injury.
Multidimensional performance measures and factors and their linkage with performance
Authors: Ahmad Kamilah , Mohamed Zabri Shafie , Atan Siti Anisah 
Published: March 2022

PurposeThis study investigates the extent to which performance measures (PMs) are used, the relationship between multidimensional PMs and firm performance and the factors related to firms' PM use.Design/methodology/approachThe authors surveyed a sample of Malaysian manufacturing firms. Participants responded to a questionnaire indicating their use of PMs, firm performance, level of managerial commitment, degree of environmental uncertainty and firm's use of technology.FindingsThe results indicate a high degree of PM use related to financial indicators, internal efficiency and customer-related metrics. The results also demonstrate that firm performance has significant positive relationships with use of PMs related to quality and customers, efficiency, innovativeness and social responsibility, as well as comprehensive PM use. Industry variation, firm size, technology use and environmental uncertainty are also significantly related to PM use.Practical implicationsPerformance measurement systems (PMSs) are an important tool for improving organisational strategy in rapidly changing markets. These findings underscore the significant role of PMSs in manufacturing firms' performance, including emerging economies. The results suggest that individual PMS approaches should align with each firm's evolving needs and the characteristics of the sector and environment in which each firm operates.Originality/valueThis study advances understandings of the contingency approach to PMSs in manufacturing environments.
A framework for fire departments' performance assessment: a systematic literature review
Authors: Komsionchi Eslamzadeh Seyed Milad , Grilo António , Espadinha-Cruz Pedro , Rodrigues João Paulo C. , Lopes José Pedro 
Published: March 2022

PurposeThe purpose of this research is to review literature about the performance assessment (PA) in urban fire departments (FDs) to gain state-of-the-art of the fire departments' performance assessment (FDPA) and identify its most applied methods and indicators.Design/methodology/approachA five-stage structured literature review (SLR) is conducted to review the FDPA-related studies; then, the statistical analysis is applied to reveal more information from the extracted data and design a general framework for FDPA.FindingsThe systematic literature review resulted in 336 independent variables for FDPA and finding the data envelopment analysis (DEA) as the most applied FDPA method among the mathematical and statistical models in the reviewed papers. By using analysis outcomes, a general conceptual framework for FDPA is proposed.Research limitations/implicationsThe reviewed studies were limited to assessments at the strategic level and urban fire protection services.Practical implicationsThe results of this research can support fire protection service managers, decision-makers, PA researchers and academicians to have a better understanding of FDPA and state-of-the-art in this field.Originality/valueA considerable number of studies have been done about the FDPA to provide methods to improve the efficiency and effectiveness of FDs. Although there are reviews about PA in fire service areas, to the best of our knowledge, no study has been done about FDPA.
Overall satisfaction formation across channels: an empirical study
Authors: Bezes Christophe 
Published: March 2022

PurposeNo other research analyzes the formation of overall satisfaction across channels, including the reciprocal interactions between store and website satisfaction and the factors that moderate them. Therefore, the aim of the present study is to examine how overall customer satisfaction is formed from the image and perceived congruence of the two main existing channels and the satisfaction induced by each.Design/methodology/approachThe online study covers 909 customers of a French mortar-to-click retailer specializing in women's and children's clothing. The conceptual model incorporates reciprocal interdependence between store satisfaction and website satisfaction. This model is tested using the procedure specific to non-recursive structural equation model.FindingsOverall satisfaction with the retailer is not only generated by satisfaction with the store and the website, but also directly and indirectly by the image of each channel. The contribution of the variables depends on the personal and situational characteristics of the customer. Not only is the relationship confirmed from store satisfaction to website satisfaction, but for the first time, in rarer cases the reverse is also observed. On the other hand, while the perceived congruence of channels can improve satisfaction with the channel for certain types of customers, in other cases the congruence can also worsen customers' overall satisfaction.Research limitations/implicationsThe detailed analysis of the formation of this satisfaction shows the pre-eminence of the customer over the retailer's initiatives. A retailer may facilitate or encourage virtuous interaction between retailer's channels by making the transfer of information and products more fluid (click and collect for example); but in the end, the customer is the one who decides whether or not to bring the channels closer together.Originality/valueContrary to what the literature assumes, in some cases, the retailer's attempt to integrate the channels may even reduce overall satisfaction if customers do not want this integration, just as a high level of satisfaction on the website can reduce in-store satisfaction.
Uncovering the affective affordances of videoconference technologies
Authors: Vidolov Simeon 
Published: March 2022

PurposeThe purpose of this study is to examine the role of videoconferencing technologies for mediating and transforming emotional experiences in virtual context.Design/methodology/approachDrawing on empirical data of video conferencing experiences, this study identifies different constitutive relations with technology through which actors cope with actual or potential anxieties in virtual meetings. It draws on the phenomenological-existential tradition (Sartre and Merleau-Ponty) and on an interpretive phenomenological analysis (IPA) to conceptualize and illustrate the role of affective affordances in virtual settings.FindingsThe study identifies four different body–technology–other relations that provide different action possibilities, both disclosing and concealing, for navigating emotional experiences in virtual encounters of mutual gazing. These findings offer insights into the anatomy of virtual emotions and provide explanations on the nature of Zoom fatigue (interactive exhaustion) and heightened feelings of self-consciousness resulting from video conferencing interactions.Originality/valueThis paper builds on and extends current scholarship on technological affordances, as well as emotions, to suggest that technologies also afford different tactics for navigating emotional experiences. Thus, this paper proposes the notion of affective affordance that can expand current information system (IS) and organization studies (OS) scholarship in important ways. The focus is on videoconference technologies and meetings that have received little research attention and even less so from a perspective on emotions. Importantly, the paper offers nuanced insights that can advance current research discourse on the relationships between technology, human body and emotions.
Dilemmatic dual-factor determinants of discontinuous intention in cryptocurrency usage
Authors: Huang Cheng-Kui , Lee Neil Chueh-An , Chen Wen-Chi 
Published: March 2022

PurposeCryptocurrency, an important application of blockchain technology, has gradually circulated, and its use has become widespread. While cryptocurrency is growing rapidly, potential risks are simultaneously emerging. Users thus may abandon their usage behavior of cryptocurrency, hindering the future development of cryptocurrency. While prior studies focus more on the intention to use cryptocurrency in the pre-adoption phase, less studies pay attention to discontinuance usage intention in the post-adoption phase. To fill this knowledge gap, this stfudy aims to explore factors that cause discontinuance usage intention regarding cryptocurrency.Design/methodology/approachBased on the net valence framework theoretically grounded on the theory of reason action, a dilemmatic dual-factor model is proposed to figure out cryptocurrency users' discontinuance usage intention from the perceived risk and perceived benefit. This study identifies four potential risks and three potential benefits that affect perceived risk and benefit. The model with nine hypotheses were developed, and research data were collected by a survey method. A total of 343 valid responses were received, and PLS-SEM with SmartPLS was utilized to test the nine hypotheses, with seven hypotheses supported empirically.FindingsOur findings demonstrate that financial, legal and operational risks are critical to increase users' perceived risk, and perceived usefulness and seamless transactions play important roles in enhancing users' perceived benefit. Moreover, while perceived risk can increase users' discontinuance usage intention to cryptocurrency, perceived benefit can mitigate such intention.Originality/valueThis study contributes nascent knowledge to the literature by examining factors that influence discontinuous usage intention in regard to cryptocurrencies, to firms that have issued or attempted to issue cryptocurrencies and to the potential users of cryptocurrencies by adjusting the mode of operation and investment strategies and reducing user costs, achieving a win-win situation for firms and users.
Understanding the choice of human resource and the artificial intelligence: “strategic behavior” and the existence of industry equilibrium
Authors: Das Dipankar 
Published: March 2022

PurposeArtificial intelligence (AI) has become an input to the production of goods and services. Therefore, a general question is there that “How the labor hour/human resource will be replaced by the artificial intelligence?” To answer this question, the paper considers that both AI and the human resources (HR) are the inputs to the firm and explains the choice between the two with reference to the customer relationship management. The paper derives the individual firms and the industry demand functions of the AI and the HR when both are present in the production of the identical or closely related goods and services. Moreover, the paper also shows the strategic behavior of an individual firm with the industry in selecting the AI and the HR. It has been shown that the individual firm's choice in the industry depends on the choice of the industry leader. The paper explains the supermodular game between the firms in an industry.Design/methodology/approachGame theory, industrial organization and non-convexity theories have been used in this paper to identify the choice between the HR and the AI in the customer relationship management.FindingsThe paper explains analytically the preference and demand for AI in the industry. Individual firm's strategic behavior and decision on choosing AI and the industry equilibrium have been studied logically. Moreover, the paper gives some light on the question of employment in presence of AI. The paper proves that in the presence of AI, labor demand will not be reduced but both will be used.Originality/valueThis work proves for the first time using some logical derivation that AI will not crowd out labor from the market. Moreover, to run AI, labor should also be used. It has been proved that to complete a job with speed and quality, both AI and HR are to be used.
Engaging older workers: the role of a supportive psychological climate
Authors: Kilroy Steven , Van de Voorde Karina , Kooij Dorien , van den Dungen Sophie 
Published: March 2022

PurposeThe purpose of this study is to investigate if a supportive psychological climate specifically aimed at older workers (i.e. employee perceptions that the organization supports and activates older workers) will result in higher levels of older workers' vitality and dedication mediated through increased levels of older workers' perceived organizational support (POS).Design/methodology/approachThe authors conducted a two-wave survey study among 209 older university employees (aged above 45 years) using structural equation modeling.FindingsThe results revealed that a change in supportive psychological climate is positively associated with a change in vitality and dedication, which was mediated by a change in POS.Practical implicationsSince workforces are aging around the world, one of the most pressing challenges for human resource managers is to find effective strategies to encourage older workers to remain engaged and active members of the workforce for as long as possible. In this study, the authors demonstrate that a supportive psychological climate for older workers is particular important in this regard.Originality/valueThe main contribution of this study is that it identifies the important role of a supportive psychological climate for older workers in predicting older workers engagement i.e. vitality and dedication, while also shedding light on the underlying mechanisms involved.
New venture top management team's shared leadership and its indirect effect on strategic performance: findings from SEM and fsQCA
Authors: Yu Jun , Ma Zhengcong , Song Wenhao 
Published: March 2022

PurposeThe purpose of this study is to empirically explore the relationship between a new venture top management team's (NVTMT’s) shared leadership and strategic performance in opportunity recognition and entrepreneurial bricolage by drawing on the upper echelons theory.Design/methodology/approachData were collected from 344 new manufacturing ventures located in Eastern China. The hypotheses were tested using structural equation modelling (SEM) through the AMOS 23.0 software package. The confluence of the contextual factors of the new venture is examined by a fuzzy-set qualitative comparative analysis (fsQCA).FindingsThe results indicate that NVTMT shared leadership has an indirect and positive effect on strategic performance through opportunity recognition, especially in a highly uncertain environment, while the mediating effect of entrepreneurial bricolage is not significant. Furthermore, although the SEM results show that the impact of NVTMT shared leadership on entrepreneurial bricolage is negative, the fsQCA shows that NVTMT shared leadership can significantly and positively affect entrepreneurial bricolage in an environment with high uncertainty, ultimately enhancing strategic performance.Originality/valueThis study contributes to the shared leadership literature by proposing a model on how shared leadership shapes the strategic performance of new ventures via opportunity recognition and entrepreneurial bricolage. The findings not only enrich relevant research on the upper echelons theory, but also help in understanding the patterns of contextual conditions that facilitate the value-adding properties of NVTMT shared leadership.