bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endothelial Progenitor Cell-Mediated Angiogenesis is Promoted by the Transforming Growth Factor Beta 1/CXC Chemokine Receptor 7 Signaling Axis in Diabetes Mellitus
Authors: Huang Zhiyong , Lei Chen , Ni Xuejun , Zhang Mingliang , Jia Xinyu , Xiao Yiming , Wei Zengni , Shan Xiuying , Wang Biao 
Published: June 2022

Abstract
Background
The role of the transforming growth factor (TGF)-β1/CXC chemokine receptor (CXCR) 7 signaling axis in angiogenesis mediated by endothelial progenitor cells (EPCs) in diabetes mellitus (DM) remains unclear.
Methods
An in vivo wound healing model was established in mice, which were treated with phosphate-buffered saline (PBS) or TGF-β1. Wound closure rate was measured to evaluate wound healing. Histological analysis and immunofluorescent staining were performed to investigate angiogenesis and the number of EPCs in vivo. EPCs were isolated from bone marrow via gradient centrifugation. An angiogenic medium was used to induce endothelial cells (ECs) from EPCs. Flow cytometry was used to identify EPCs and ECs. Lv-shCXCR7 was used to determine the role of CXCR7 in EPCs in vitro. EPC proliferation was measured via 5-ethynyl-2′-deoxyuridine staining. Scratch and transwell assays were performed to assess EPC migration.
Results
Remaining wound area from day 7 to 14 in the DM + TGF-β1 group was significantly lower than that in the DM + PBS group but higher than that in the PBS group. On day 14, the number of microvessels in wounds was significantly higher for TGF-β1-treated mice than for mice not treated with TGF-β1. The number of EPCs in the DM + TGF-β1 group was significantly higher than that in the DM + PBS group. The high-glucose environment significantly impaired the proliferation, migration, and tube-forming ability of EPCs; however, TGF-β1 partially restored the proliferation and tube-forming ability of EPCs (p < 0.05). Additionally, shCXCR7 lentiviral transfection resulted in a significant reduction in the proliferation and tube-forming ability of EPCs; however, it had no significant effect on EPC migration. Furthermore, the high-glucose environment caused a decrease in the mRNA and protein expression of CXCR7 in EPCs; however, this was partially reversed by TGF-β1 (p < 0.05).
Conclusions
Our results show for the first time that TGF-β1 partially restores the proliferation and tube-forming ability of EPCs in a high-glucose environment by upregulating CXCR7 expression, increasing the number of blood vessels, and accelerating wound healing. This novel TGF-β1 pathway may play a potentially therapeutic role from bench to bedside.
Performance analysis of a versatile bulk-service queue with group-arrival, batch-size-dependent service time and queue-length dependent vacation
Authors: Pradhan Sourav , Nandy Nilanjan , Gupta Umesh Chandra 
Published: June 2022

Abstract
Queueing systems with bulk-service and vacation policy have become one of the pivotal interest for the researchers due to their widespread application in food processing technology, manufacturing systems etc. This article analyzes a single server versatile bulk-service queueing system wherein the customers arrive according to compound Poisson process and service time is dependent on the batch-size of undergoing service. Moreover, single and multiple vacation policy have been incorporated along with queue-length dependent vacation. After providing the steady-state system equations, the bivariate probability generating function of queue and server content distribution together has been derived at departure epoch. After the evaluation of the unknown probabilities , complete joint distribution has been extracted in terms of roots of the denominator of the bivariate pgf. The joint distribution at arbitrary epoch have also been reported. The discussed procedure and the reported results have been depicted through some numerical examples for different service and vacation time distributions. Some significant observation about the model have been sketched graphically.MSC Classification: 60G05 , 60K25
Legume Based Traditional foods of Palampur region of Himachal Pradesh and their nutritional composition
Authors: sharma rekha 
Published: June 2022

Abstract
The present investigation was undertaken with the objectives to document the traditional food recipes, process/preparation, nutritional evaluation and development of protocols for the standardization of the recipes to minimize the nutrient losses. Three blocks viz. Baijnath, Panchrukhi, Bhawarna and Palampur itself were selected. A questionnaire was used to collect the relevant information on traditional foods from 120 respondents. In legumes black gram contained highest crude protein content(26.26%).Recipes analysis result showed that pakodu contained high crude protein content i.e. 27.44 per cent, whereas Crude fat content was highest in mukund badi madra (22.17%).
Contemporaneous sample data tracking for the generation of genome edited cell lines
Authors: Plant Anne L. , Halter Michael W. , Stinson Jeffrey R. , Greene Gretchen R. 
Published: June 2022

Abstract
It is difficult to capture the large numbers of steps and details that often characterize research in the biomedical sciences. We present an approach that is based on commercial spreadsheet software so it is easily adaptable by the experimentalist. The approach is designed to be compatible with an experimentalist’s workflow and allows the capture in real time of detailed information associated, in this use case, with laboratory actions involved in the process of editing, enriching and isolating clonal gene-edited pluripotent stem cell (PSC) lines. Intuitive features and flexibility allow an experimentalist without extensive programming knowledge to modify spreadsheets in response to changes in protocols and to perform simple queries. The experimental details are collated in a table format from which they can be exported in open standard formats (e.g., Extensible Markup Language (XML) or Comma Separated Values (CSV) for ingestion into a data repository supporting interoperability with other applications. We demonstrate a sample- and file-naming convention that enables the automated creation of file directory folders with human readable semantic titles within a local file system. These operations facilitate the local organization of documentation and data for each cell line derived from each transfection in designated folder/file locations. This approach is generalizable to experimental applications beyond this use case.
Exergy Assessment of Hybrid District Heating Systems Using Resource Exergy Analysis (REA)
Authors: Jentsch Andrej , Yu Young Jae , Ramousse Julien , Kallert Anna 
Published: June 2022

Abstract
In this paper six supply systems for a simulated energy demand scenario are compared using resource exergy analysis (REA). The analysis is complemented with an assessment of greenhouse gas emissions. The six scenarios (S) that are compared are • S1: decentralized gas boilers, • S2: low temperature district heating using 50% heat from a block CHP plant and 50% heat from a gas boiler, • S3: decentralized air-source heat pumps, • S4: a very low temperature district heating network with a central large heat pump and decentralized electrical boilers, • S5: a cold district heating network using heat from the ground as a source for decentralized water-water heat pumps and • S6: deep geothermal low temperature district heating network. The electricity used in all scenarios is assumed to come from PV panels that are newly built on the district. Thus, all results for hybrid energy systems can be considered best-case scenarios. Scenario 1 and Scenario 6 are intended to provide reference scenarios for the evaluation of the considered hybrid energy systems. S1 allows a comparison with the current status quo. S6 is there to show how good hybrid energy systems can be if compared to one of the best thermal district heating sources. The results of the performed analysis show that hybrid energy networks can be among the most resource saving and low carbon heat supply solutions possible. In order to achieve this outcome, it is important to ensure that the electric load generated by these systems is covered by additional GHGE-free power supply. In comparison to natural gas boilers hybrid energy systems can save more than 70% of resource exergy and over 90% of GHGE. All considered hybrid energy systems produce similar savings, so that the decision on what type of hybrid energy system is best for a given community largely depends on the heat demand density, the potential for heat networks or air-water heat exchangers and the availability of suitable heat sources apart from life-cycle cost considerations. While hybrid energy networks can be among the top solutions for decreasing resource exergy consumption and GHGE, they are not the only technology suitable for resource saving and climate friendly heat supply. Scenario 6 (Deep geothermal heat) shows that district heating using suitable thermal sources can match or even outperform best case hybrid energy networks. However, since thermal sources that can directly provide heat at the temperature levels required by the building stock can be locally limited, hybrid energy networks are one of the key technologies to supply heat to areas with high heat demand density.
WIDESim: A toolkit for simulating resource management techniques of scientific Workflows in Distributed Environments with graph topology
Authors: Rayej Mohammad Amin , Siar Hajar , Izadi Mohammad 
Published: June 2022

Abstract
This paper presents a toolkit for simulating resource management of scientific workflows in distributed environments with graph topol-ogy called WIDESim. WIDESim can work with all three different structures of scientific workflows: single, multiple workflows, and workflow ensembles. Also, unlike most existing network simulators, there is no constraint on the topology of the distributed environment. We have analyzed the performance of WIDESim in comparison to standard simulators and workflow management tools. The comparison indicates that WIDESim’s performance is close to existing standard simulators besides its improvements.
Photo-engineered optoelectronic properties of indium tin oxide via reactive laser annealing
Authors: Hillier James Athur , Patsalas Panos , Karfaridis Dimitrios , Camelio Sophie , Cranton Wayne , Nabok Alexei V. , Mellor Christopher J. , Koutsogeorgis Demosthenes C. , Kalfagiannis Nikolaos 
Published: June 2022

Abstract
Transparent conductive oxides are appealing materials for optoelectronic and plasmonic applications as, amongst other advantages, their properties can be modulated by engineering their defects. Optimisation of this adjustment is, however, a complex design problem. This work examined the modification of the carrier transport properties of sputtered tin-doped indium oxide (ITO) via laser annealing in reactive environments. We relate the optical modifications to the structural, compositional, and electronic properties to elucidate the precise mechanisms behind the reactive laser annealing (ReLA) process. For sufficiently high laser fluence, we reveal an ambient-dependent and purely compositional modulation of the carrier concentration of ITO thin films. Hereby, we demonstrate that ReLA utilises the precise energy delivery of photonic processing to enhance the carrier mobility and finely tune the carrier concentration without significantly affecting the crystal structure. Exploitation of this phenomena may enable one to selectively engineer the optoelectronic properties of ITO, promising an alternative to the exploration of new materials for optoelectronic and photonic applications.
Validation of Indian version of musculoskeletal tumor society score questionnaire- hospital- based cohort study
Authors: Ansari Sajid , Khande Chandra Kumar , Bansal Shivam , sah Saroj prasad , khan Mohd Moin , dhingra mohit 
Published: June 2022

Abstract
Background- Scoring systems are regarded as quantitative methods to assess the severity of a disease and its treatment modalities. The Musculoskeletal tumor society score (MSTS) questionnaire which was developed in 1985 and revised in 1993 to measure the functional outcome in patients with neoplasm still holds good. All scoring systems need validation in the native language of the people being used. Here, we have created an Indian version of the MSTS scoring system and validated the psychometric properties of the created version. Material and methods- all the patients who were operated on for aggressive benign and malignant bone tumors of lower limbs were included in the study. two independent observers who were well versed in the Hindi language made a consensual draft. the questionnaire was put to the patients at two different point of time and the results were analysed. Results- the lower extremity version demonstrated an excellent internal consistency (Cronbach's alfa = 0.999: CI = 0.9-1.0) at day 0 and excellent reliability (Cronbach alfa = 0.999: CI = 0.9-1.0) except for good reliability (Cronbach's alfa = 0.87: CI = 0.80–0.92) of gait score at day 10. Conclusion - from the above validation we conclude that the Hindi version of the MSTS score can be easily used in place of the English in our Indian population
First-trimester abortion protocols in hospitals compared to office-based settings in Switzerland – a national survey study in Switzerland
Authors: Eckstein Samuel Martin , von Felten Stefanie , Perotto Laura , Brun Romana , Vorburger Denise 
Published: June 2022

Abstract
PurposeSimplified first-trimester abortion protocols are well established as they facilitate access to services and improve patient satisfaction without compromising safety. However, data on abortion protocols across Switzerland by facility type are lacking. Therefore, the first aim of this study was to analyse hospital-derived abortion protocols, as opposed to office-based protocols, across Switzerland in terms of their acceptance among women. The second aim was to assess abortion protocols and outcomes of specialised general practitioners (GPs) following simplified protocols.MethodsThis study consists of two parts. (i) A nationwide questionnaire was distributed among hospitals and private practices covering details of abortion protocols and numbers between April and July, 2019. The primary endpoint was the proportion of women undergoing an abortion after the first consultation at the chosen facility (adherence), regarding the complexity of abortion protocols in each setting. (ii) A retrospective study was conducted to analyse the outcomes of office-based institutions with simplified abortion procedures over a period of 10 years from January, 2008, to December, 2018. (BASEC-No. Req-2018-01031).Results (i) Office-based facilities showed less complex abortion protocols, resulting in a significantly higher probability of undergoing an abortion after the first consultation than hospitals. Their protocols allowed for a higher gestational age by medical means, and they offered mifepristone intake within the first consultation along with fewer staff members involved and a lower number of overall consultations. (ii) From 5,495 women, a total of 5,274 patients were included, of which the incidence of complications requiring surgery was at a low of 2.5%. ConclusionThis study highlights that simplified abortion protocols are crucial to ease access to abortion services, as represented by higher adherence in office-based facilities. Simplified protocols in abortion services are safe, and repetitive counselling and in-person visits should only be reserved for selected cases.
Short Communication: How Should the Respiration Rate Be Counted in Cattle?
Authors: Dißmann L. , Heinicke J. , Jensen K. C. , Amon T. , Hoffmann G. 
Published: June 2022

Abstract
Respiration rate (RR) is a proficient indicator to measure the health status of cattle. The common method of measurement is to count the number of respiratory cycles each minute based on flank movements. However, there is no consistent method of execution. In previous studies, various methods have been described, including counting flank movements for 15 s, 30 s or 60 s as well as stopping the time for 5 or 10 breaths. We assume that the accuracy of the aforementioned methods differs. Therefore, we compared their precision with an RR sensor, which was used as the reference method in this study. Five scientists from the fields of agricultural science and veterinary medicine quantified the flank movement according to each of the five methods mentioned above. The results showed that with an average RR of 30 breaths per minute (bpm), all methods showed a high correlation to the values of the RR sensor. However, counting breaths for 60 s had the highest level of conformity with the RR sensor (Lin`s concordance correlation coefficient: 0.96) regardless of the level of RR. With rising RR, the inaccuracy increased significantly for the other four investigated methods, especially when counting 5 and 10 breaths. Therefore, we would recommend that counting for 60 s should be used as the standard method for future studies due to its high precision regardless of the level of RR.
Preparation of Metrological States in Dipolar-Interacting Spin Systems
Authors: Maurer Peter , Zheng Tian-Xing , Li Anran , Rosen Jude , Zhou Sisi , Koppenhoefer Martin , Ma Ziqi , Chong Fred , Clerk Aashish , Jiang Liang 
Published: June 2022

Abstract
Spin systems are an attractive candidate for quantum-enhanced metrology. Here we develop a variational method to generate metrological states in small dipolar-interacting spin ensembles with limited qubit control, as present in nansocale quantum sensing applications. For both regular and disordered spatial spin configurations the generated states enable sensing beyond the standard quantum limit (SQL) and, for small spin numbers, approach the Heisenberg limit (HL). Depending on the circuit depth and the level of readout noise, the resulting states resemble Greenberger-Horne-Zeilinger (GHZ) states or Spin Squeezed States (SSS). Sensing beyond the SQL holds in the presence of finite spin polarization and a non-Markovian noise environment.
Synthesis, Chemical, Morphological and Thermal Characterization Yeast Immobilized Bacterial Cellulose and It Use in Color Removal
Authors: BORAN Filiz 
Published: June 2022

Abstract
Bacterial cellulose is a biomaterial and can be used for many applications such as medical products and food ingredients with its superior properties such as being pure, high crystallinity and durability. It can also be used as an as an immobilization support. It is a good and practical support for yeast immobilization. The present study aimed to immobilize yeast on lyophilized bacterial cellulose produced by Gluconacetobacter xylinus B759 in cheese whey medium as a cheap and natural culture medium. Optimum conditions such as cultivation time, carbon source, temperature, pH and nitrogen sources for bacterial cellulose production in cheese whey medium were determined. The highest bacterial cellulose yield obtained under optimized conditions was 13.18 g/L. Saccharomyces cerevisiae cells were immobilized on purified and lyophilized 0.5 cm pure bacterial cellulose. Bacterial cellulose and yeast-immobilized bacterial cellulose structures were characterized by different instrumental techniques. Furthermore, Reactive Blue 171 dye decolorization ability of immobilized yeast was tested. The structure of bacterial cellulose showed very suitable morphology and chemical structure for different biotechnological applications. The use of a cheap and natural medium is very important in terms of production conditions, as it is an environmentally friendly application in terms of biotechnology.
A human-machine collaborative video summarization framework using pupillary response signals
Authors: Ma Haigang , Yu Wenyuan , Wang Yaoyao , Ding Nai , Zheng Jing 
Published: June 2022

Abstract
This paper proposes a novel, human-machine collaborative, pupillary response-based video summarization framework. Considering that humans are the end-users and evaluators of video content, it is natural to establish the links between video features and real-time viewers’ attentive response for designing a video summarization framework. In this paper, pupil size, a pupillary response, is introduced as a real-time indicator for assessment of viewers’ engagement and attention. Firstly, we augmented the TVSum dataset by replacing manual annotations with attention scores that converted from pupillary size signals to generate the perceptually driven dataset. Secondly, we developed a video summarization framework which uses cues from pupillary size signal of humans to predict frame-level attention scores, and then extracts key shots from videos. On the perception-driven dataset, the average F-measure of the proposed summarization method is 69.71%, while the precision and recall are 69.67% and 69.77%, respectively, which is a significant improvement compared to random summarization. Experimental results initially demonstrated that our modeling approach can learn the dynamic attention mechanism of viewers and apply it to video summarization. In addition, the experimental results validate the effect.
LncRNA AOC4P impacts the differentiation of macrophages and T-lymphocyte by regulating the NF-κB pathways of KGN cells: potential pathogenesis of polycystic ovary syndrome
Authors: Wu Shujuan , Yang Zhe , He Shaojing , Han Lu , Zhou Mengqi , Yang Jing , Chen Jiao , Wu Gengxiang 
Published: June 2022

Abstract
Background
Polycystic ovary syndrome (PCOS) is a multifactorial endocrine disease, which is an important cause of female infertility worldwide. PCOS patients are in a state of chronic low-grade inflammation, and immune imbalance is considered as a potential cause of its pathogenesis.
Methods
The expression of AOC4P in PCOS and normal ovarian granulosa cells (GCs) was detected by real-time quantitative PCR. KGN cells were induced by dihydrotestosterone at 500 ng/ml to construct PCOS model. After lentivirus-infected, KGN cells were constructed with AOC4P overexpression cell lines, the proliferation and apoptosis levels of KGN cells in AOC4P and NC groups were detected. THP-1-derived macrophages and peripheral blood mononuclear cells (PBMC) were co-cultured with KGN cells for 48h respectively, and the differentiation of macrophages and CD4 + T cells were detected by flow cytometry.
Results
Decreased AOC4P expression was found in PCOS patients. After constructing PCOS cell model, we observed that overexpression of AOC4P promoted KGN cell proliferation and inhibited apoptosis. After co-culture with AOC4P overexpressed KGN cells, M1 macrophages decreased, M2 macrophages increased, Th1/Th2 ratio increased, and Treg cells increased. Finally, we found that AOC4P inhibited the activation of the nuclear factor κ B (NFκB) pathway in KGN cells.
Conclusions
In this study, we found that AOC4P regulated the NFκB signaling pathway by inhibiting the phosphorylation of P65, thereby affecting the proliferation and apoptosis of GCs, altering the differentiation of macrophages and T cells, and contributing to the pathogenesis of PCOS.