bluuebunny/crossref_metadata_2025_split huggingface dataset:
Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via “flow‐through affinity chromatography” using peptide‐based adsorbents
Authors: Sripada Sobhana Alekhya , Chu Wenning , Williams Taufika Islam , Teten Matthew A. , Mosley Brian J. , Carbonell Ruben G. , Lenhoff Abraham M. , Cramer Steven M. , Bill Jerome , Yigzaw Yinges , Roush David J. , Menegatti Stefano 
Published: April 2022

AbstractThe growth of advanced analytics in manufacturing monoclonal antibodies (mAbs) has highlighted the challenges associated with the clearance of host cell proteins (HCPs). Of special concern is the removal of “persistent” HCPs, including immunogenic and mAb‐degrading proteins, that co‐elute from the Protein A resin and can escape the polishing steps. Responding to this challenge, we introduced an ensemble of peptide ligands that target the HCPs in Chinese hamster ovary (CHO) cell culture fluids and enable mAb purification via flow‐through affinity chromatography. This study describes their integration into LigaGuard™, an affinity adsorbent featuring an equilibrium binding capacity of ~30 mg of HCPs per mL of resin as well as dynamic capacities up to 16 and 22 mg/ml at 1‐ and 2‐min residence times, respectively. When evaluated against cell culture harvests with different mAb and HCP titers and properties, LigaGuard™ afforded high HCP clearance, with logarithmic removal values (LRVs) up to 1.5, and mAb yield above 90%. Proteomic analysis of the effluents confirmed the removal of high‐risk HCPs, including cathepsins, histones, glutathione‐S transferase, and lipoprotein lipases. Finally, combining LigaGuard™ for HCP removal with affinity adsorbents for product capture afforded a global mAb yield of 85%, and HCP and DNA LRVs > 4.
Optimization of continuous TAG production by Nannochloropsis gaditana in solar‐nitrogen‐limited culture
Authors: Heredia Vladimir , Marchal Luc , Gonçalves Olivier , Pruvost Jeremy 
Published: April 2022

AbstractNitrogen limitation and changing solar conditions are both known to affect triacylglycerol (TAG) production in microalgae. This study investigates the optimization of TAG production with a continuous nitrogen‐limited culture of Nannochloropsis gaditana in simulated day–night cycles (DNc). The effect of DNc was first investigated in nitrogen‐deprived condition (i.e., batch culture), emphasizing a significant change in mechanical resistance of the strain during the night. The concept of released TAG, which shows how much of the TAG produced is actually recovered in the downstream stages, that is, after cell disruption, was shown here of interest. For a maximum released TAG, the optimum harvesting time was suggested as being 4 h into the night period, which minimizes the losses due to a too great cell mechanical resistance. The protocol for continuous nitrogen‐limited culture was then optimized, and a continuous nitrogen addition was compared to a pulsed‐addition. For the latter, nitrogen was supplied in a single pulse at the beginning of the light periods, while the bulk medium was supplied separately at a slow but constant dilution rate of . The pulse dose was calculated after the study of nitrogen consumption and TAG production/consumption during the DNc. The estimated released TAG for the pulsed‐addition of 1.4 × 10−3 kg/m2d found significantly higher than the one achieved in batch culture (0.3 × 10−3 kg/m2d) but lower than for continuous nitrogen addition which obtained the highest released TAG of .
A bioprinted complex tissue model for myotendinous junction with biochemical and biophysical cues
Authors: Kim Won Jin , Kim Geun Hyung 
Published: April 2022

AbstractIn the musculoskeletal system, the myotendinous junction (MTJ) is optimally designed from the aspect of force transmission generated from a muscle through a tendon onto the bone to induce movement. Although the MTJ is a key complex tissue in force transmission, the realistic fabrication, and formation of complex tissues can be limited. To obtain the MTJ construct, we prepared two bioinks, muscle‐ and tendon‐derived decellularized extracellular matrix (dECM), which can induce myogenic and tenogenic differentiation of human adipose‐derived stem cells (hASCs). By using a modified bioprinting process supplemented with a nozzle consisting of a single‐core channel and double‐sheath channels, we can achieve three different types of MTJ units, composed of muscle, tendon, and interface zones. Our results indicated that the bioprinted dECM‐based constructs induced hASCs to myogenic and tenogenic differentiation. In addition, a significantly higher MTJ‐associated gene expression was detected at the MTJ interface with a cell‐mixing zone than in the other interface models. Based on the results, the bioprinted MTJ model can be a potential platform for understanding the interaction between muscle and tendon cells, and even the bioprinting method can be extensively applied to obtain complex tissues.
Perceptions of telehealth in real‐world oncological care: An exploration of matched patient‐ and clinician‐reported acceptability data from an Australian cancer centre
Authors: Collins Anna , McLachlan Sue‐Anne , Pasanen Leeanne , Wawryk Olivia , Philip Jennifer 
Published: April 2022

AbstractBackgroundPrior to 2020, the use of telehealth in cancer care was limited, but COVID‐19 necessitated its rapid and widespread adoption into routine care delivery. This study aimed to evaluate perceptions of telehealth through a dyadic exploration of matched cancer patient‐ and clinician‐reported acceptability data and to explore factors that may predict greater suitability for telehealth.MethodsA prospective, cross‐sectional, exploratory survey study assessed (matched) patient‐ and clinician‐reported perceptions of telehealth consultations occurring at a metropolitan, tertiary‐based cancer centre in Victoria, Australia.ResultsOne‐hundred and fifty‐five matched patient‐ and clinician‐reported data were included. High rates of acceptability with telehealth were reported by patients (93%) and clinicians (91%), who mostly shared concordant views (86%). Factors significantly associated with increased acceptability for telehealth, included, for clinicians, greater familiarity with the patient (OR 8.20, 95% CI: 1.50–45.06, p = 0.02), and younger patient age (OR 1.06, 95% CI: 0.99–1.13, p = 0.05), and for patients was earlier stage disease (≤stage III) (OR 5.29, 95% CI: 1.08–25.82, p = 0.04). Lower acceptability for telehealth according to clinicians was associated with poorer patient performance status (OR 0.04, 95% CI 1.00–1.08, p = 0.04) and for patients with the need for an interpreter (0R 0.06, 95% CI: 0.008–0.51, p = 0.009).ConclusionWhile overall telehealth is acceptable in cancer care, our findings raise important implications for future service development, notably that it may be less optimal for patients with higher complexity of need—including those with more advanced disease, poorer performance status, those less well known to treating clinicians and those identified to have additional language barriers.
Child welfare worker wellness: An ethical imperative in the service of children
Authors: Bowman M. Elizabeth 
Published: April 2022

AbstractChild welfare social workers are at high risk of burnout due to the demanding nature of their work (Bowman, 2020; Lizano & Mor Barak, 2012; Sprang et al., 2011; Schelbe, 2017). This condition can have drastic consequences for the psychosocial and emotional wellbeing of social workers and the vulnerable children and families they serve in child welfare organisations across the world. However, burnout and the associated risk of turnover and reduced self‐efficacy can be prevented through effective workplace support and intervention (Brode, 1990). This article provides an overview on the impact of burnout on child welfare workers' health, mental health and turnover as well as child and family outcomes, while underscoring the organisation's role in mitigating this issue. From an extensive literature review, the author proposes the following organisational child welfare wellness model: workplace wellness in child welfare organisations. Recommendations within this model focus on child welfare practices which provide support to workers including wellness (i.e. on‐site crisis and mental health support) and growth‐needs‐focused interventions (i.e. supervision, specialisation and promotion).
Disparities in use of disciplinary solitary confinement by mental health diagnosis, race, sexual orientation and sex: Results from a national survey in the United States of America
Authors: Henry Brandy F. 
Published: April 2022

AbstractBackgroundSolitary confinement is still used in prisons in the USA, despite its links to poor health. Past research suggests that there may be disparities by race, ethnicity, sex and mental disorders regarding who is placed in solitary confinement, although nationwide studies have been sparse.AimsTo explore possible disparities by race, ethnicity, sex, sexual orientation, adverse childhood experiences and mental disorders in solitary confinement as a disciplinary action for adults incarcerated in USA prisons.MethodsData come from a recently released national survey of 24,848 adults incarcerated in the USA—the 2016 Survey of Prison Inmates. Logistic regression models were used to identify disparities in the use of disciplinary action and solitary confinement as a disciplinary action, while controlling for type of rule violation.ResultsAfter controlling for rule violation type, solitary confinement was used as a disciplinary action at higher rates for people who: were multiracial, as compared to white (aOR = 1.30), male, as compared to female (aOR = 1.46), bisexual, as compared to heterosexual (aOR = 1.64), had multiple mental disorders, as compared to none (aOR = 1.22) or had more adverse childhood experiences (aOR = 1.13).ConclusionsFindings highlight demographic and health disparities in the use of solitary confinement, which may further widen health disparities. More effective implementation of policies to reduce the use of solitary confinement are still needed. Mental health professionals should have an active role in advising on measures when mental disorder is a factor and must ensure adequate treatment of disorders in prison or transfer to health facilities.
When coronary imaging and physiology are discordant, how best to manage coronary lesions? An appraisal of the clinical evidence
Authors: Fernández‐Peregrina Estefania , Ahmad Huzaifa , Mintz Gary S. , Garcia‐Garcia Hector M. 
Published: April 2022

AbstractBackgroundDiscordant physiology and anatomy may occur when nonsevere angiographic stenosis has positive physiology as well as the opposite situation.AimTo underline the reasons behind the discrepancy in physiology and anatomy and to summarize the information that coronary imaging may add to physiology.MethodsA review of the published literature on physiology and intravascular imaging assessment of intermediate lesions was carried out.ResultsThe limitations of angiography, the possibility of an underlying diffuse disease, the presence of a “grey zone” in both techniques, the amount of myocardial mass that subtends the stenosis, and plaque vulnerability may play a role in such discrepancy. Intracoronary imaging has a poor diagnostic accuracy compared to physiology. However, it may add information about plaque vulnerability that might be useful in deciding whether to treat or not a certain lesion.ConclusionsCoronary revascularization is recommended for patients with ischemia based on physiology. Intracoronary imaging adds information on plaque vulnerability and can help on the decision whether to revascularize or not a lesion.
Management of pain due to cervical multilevel disk bulges and spinal stenosis with a focused vibro‐percussion wave treatment: A case report
Authors: Abu Omar Arwa Jameel , Al Baradie Mohammad Sami , Al Dera Hussain , Vannabouathong Christopher , Bartel Lee 
Published: April 2022

AbstractA patient presenting with low back pain received 18 treatments of FDA‐approved low‐frequency vibro‐percussion wave stimulation known as Khan Kinetic Treatment (KKT). Following KKT, he demonstrated improvement in pain, function, quality of life, sleep, and trunk range of motion with no adverse events.
A case of bone lesion in a patient with relapsed chronic lymphocytic leukemia and review of the literature
Authors: Bacchiarri Francesca , Gozzetti Alessandro , Mondanelli Nicola , Lazzi Stefano , Bocchia Monica 
Published: April 2022

AbstractSkeletal involvement in CLL is very rare. We present a case of ileum bone lesion during in a patient receiving 5th line of therapy. Despite radiotherapy and salvage therapies, subsequent bone lesions led to a fatal outcome. Further studies on the mechanism by which bone disease develops are currently needed.
Unusual presentation of intramuscular hemangioma in abdominal oblique muscles
Authors: Miro Jameel 
Published: April 2022

AbstractIntramuscular hemangiomas are rare benign tumors that are difficult to diagnose. We report a successful case of intramuscular hemangioma excision involving the external oblique muscles. The mass was excised successfully, and histopathology confirmed the diagnosis of intramuscular hemangioma with a negative margin and no malignancy.
Novel compound heterozygous mutations in a GNE myopathy with congenital thrombocytopenia: A case report and literature review
Authors: Xu Zhouwei , Xiang Jingyan , Luan Xinghua , Geng Zhi , Cao Li 
Published: April 2022

AbstractWe reported a GNE myopathy with congenital thrombocytopenia on a young male patient. He presented with a 3‐year history of lower distal extremity weakness initially affecting his legs. The weakness slowly progressed to lower proximal legs and upper arms last 6 months. Whole‐exome sequencing revealed that the patient harbored two heterozygous gene mutations, including a novel insertion mutation c.*1037_*1038CACACACACACACACACACACA and c.C478T in exome 12 and 3 of the GNE gene (NM_001128227), respectively. The levels of serum sialic acid in this patient were considerably decreased. Muscle MRI imaging showed the anterior and medial parts of his quadriceps were heavily affected by this disease. Hematoxylin and eosin staining showed prominent rimmed vacuoles with a lack of inflammatory response in the atrophied muscle. We also undertook a review of the current literature, searching for reports in which the GNE gene mutation caused the thrombocytopenia with or without muscle weakness. This new gene mutation finding broadens the GNE disease genotype spectrum, and further investigation of the relationship between GNE gene mutations and the heterogeneity of its clinical manifestations is needed.
Uncommon presentation of Kikuchi disease
Authors: Furusaka Takayuki , Miyagami Taiju , Suzuki Mayu , Naito Toshio 
Published: April 2022

AbstractKikuchi disease is common among Asians and women aged about 30 years. General symptoms include cervical lymphadenopathy and fever; 64% of the cases are self‐limiting. However, this case is axillary lymphadenopathy, which is the main symptom in males. Thus, Kikuchi disease may be considered in the differential diagnosis of axillary lymphadenopathy.
A Facile and Environmental‐Friendly Approach to Synthesize S‐Doped Fe/Ni Layered Double Hydroxide Catalyst with High Oxygen Evolution Reaction Efficiency in Water Splitting
Authors: Xie Shijie , Liu Changsheng , Song Runwei , Ji Yingxi , Xiao Zhaohui , Huo Chunqing , Lin Shiwei 
Published: April 2022

AbstractHere we report a time‐saving and environmental‐friendly approach to grow sulfur doping Fe/Ni layered double hydroxide (S−FeNi LDH) catalysts on FeNi alloy sheet in five minutes at ambient temperature. It efficiently synthesizes a catalyst with multi‐layer and superlative oxygen evolution reaction (OER) activity in water splitting. The S−FeNi LDH shows overpotentials of 202 and 266 mV to deliver current densities of 10 and 100 mA/cm2 in alkaline water respectively. The introduction of sulfur makes the catalyst more hydrophilic and changes the adsorption energy of the OER intermediates that is performed by density functional theory (DFT). This work provides an efficient strategy to improve the catalytic activity by increasing active sites and supplies a cost‐saving method to construct an effective OER catalyst for water splitting, which is extremely important for the hydrogen economy as well as environmental conservation.
Forage nutritive value of stock‐piled cicer milkvetch for late‐season grazing
Authors: Peel Michael D. , Waldron Blair L. 
Published: April 2022

AbstractAchieving high forage production combined with adequate forage nutritive value on Intermountain western U.S. grazing lands during the fall and early winter can be difficult. Crude protein (CP) and neutral detergent fiber (NDF) of cicer milkvetch (Astragalus cicer L.) were characterized relative to alfalfa (Medicago sativa L.), and crested wheatgrass [Agropyron cristatum (L.) Gaertn.]. Forage was harvested monthly September–December from season long stockpiled growth (full‐season), stockpiled regrowth following a June defoliation (mid‐season), and stockpiled regrowth following an August defoliation (late‐season). Crude protein of cicer milkvetch ranged from 11.9 to 9.2%, 20.5 to 14.3%, and 24.6 to 15.9%, in the full‐, mid‐, and late‐season stockpiled strategies, respectively. Overall, cicer milkvetch CP was equal to alfalfa CP and 5.7 percentage points greater than crested wheatgrass CP across all harvest dates of the three stockpiling scenarios. Furthermore, cicer milkvetch NDF was less (more favorable) than both alfalfa and crested wheatgrass, ranging from 46.4 to 59.6%, 23.0 to 37.4%, and 26.2 to 33.7%, in the full‐, mid‐, and late‐season stockpiled strategies, respectively. The high CP and superior NDF of cicer milkvetch indicate it has unrealized potential for improving forage nutritive value of grazing lands in the western United States, particularly in the late fall to early winter.
A survey of turfgrass irrigation water quality in Pennsylvania
Authors: Landschoot Peter James , Spargo John , McGraw Benjamin A. 
Published: April 2022

AbstractSurveys of irrigation water reveal important information about water quality in different geographic regions and can serve as a reference to compare with individual samples submitted to labs. This survey aimed to establish a baseline profile of turfgrass irrigation water quality from nonamended and noneffluent sources in Pennsylvania that can be compared with water quality data from other geographic regions and future surveys in the Mid‐Atlantic region, and to improve test report guidelines for samples submitted to labs. The survey was conducted between fall 2007 and spring 2019 and included 190 irrigation water samples. Samples were analyzed at Penn State's Agricultural Analytical Services Laboratory (PSAASL) for pH, alkalinity, hardness, electrical conductivity, total dissolved solids, sodium absorption ratio, bicarbonate, carbonate, residual sodium carbonate, nitrate‐N, ammonium‐N, and 13 elements. The results showed acceptable irrigation water quality according to published guidelines, with some exceptions, which included high (and potentially problematic) values for sodium, total dissolved solids, and sulfur, but these represented ≤10% of the samples. According to the results, most normal ranges and upper limits for the quality parameters of turfgrass irrigation water should be similar to those currently listed in PSAASL reports. However, the normal ranges for pH, alkalinity, and bicarbonate should be expanded to account for the numerous water samples (> 45%) that exceeded the maximum range listed in test reports. The normal range and upper limit of nitrate‐N should be lowered in PSAASL reports to reflect the results and concerns about environmental and health‐related effects.
Zwitterionic Block Copolymers for the Synthesis and Stabilization of Perovskite Nanocrystals
Authors: Cueto Christopher , Donoghue Colleen , Bolduc Kimberly , Emrick Todd 
Published: April 2022

AbstractTraditional hot injection methods for the preparation of cesium lead halide perovskite nanocrystals (CsPbX3 PNCs, where X=Cl, Br, or I) rely on small molecule surfactants to produce PNCs with cube, plate, or rod‐like morphologies. Here, we describe a new method whereby zwitterionic block copolymers are employed as macromolecular ligands in PNC synthesis, affording PNCs with excellent colloidal stability, high photoluminescence quantum yield, and in some cases distinctly non‐cubic shapes. The block copolymers used in this study – composed of a poly(n‐butyl methacrylate) hydrophobic block and zwitterionic methacrylate hydrophilic blocks – dissolve in useful solvents for PNC growth despite containing large mole percentages of zwitterionic groups. PNCs prepared with block copolymer ligands were found to disperse and retain their fluorescence in a range of polar organic solvents and were amenable to direct integration into optically transparent nanocomposite thin films with high PNC content.
Tieftemperaturkühlung in der Pharma‐ und Biotechnologie
Authors: Schäble Pia 
Published: April 2022

AbstractDa es kaum wirtschaftliche Alternativen zum Erreichen sehr niedrigen Temperaturen gibt, darf R23 trotz des hohen GWP‐Werts noch in Ausnahmen eingesetzt werden. Für mehr Planungssicherheit bietet der Anlagenbauer KIT‐Plersch nun eine umweltfreundliche und effiziente Lösung auf Basis einer Kaskadentechnologie mit natürlichen Kältemitteln an.
Vom Labor bis in den Produktionsprozess
Authors: Cuellar Maryann 
Published: April 2022

AbstractDie Raman‐Spektroskopie ist in der biopharmazeutischen Industrie inzwischen ein Verfahren mit einer langen Anwendungsgeschichte. Die Technologie hat sich mit der Branche weiterentwickelt, und die im Markt verfügbaren Analysegeräte sind die Antwort auf viele verfahrenstechnische Herausforderungen in biopharmazeutischen Upstream‐ wie Downstream‐Prozessen.
Hygienische Doppelschraubenspindelpumpe fördert Desinfektionsgel
Authors: Kochenburger Nicole 
Published: April 2022

AbstractAufgrund des weiterhin hohen Bedarfs an Desinfektionsmitteln hat die Steinfels Swiss in ihre Produktionsanlagen investiert. Im Werk in Winterthur wurde im Rahmen des Umbaus eine neue Pumpe von Lewa Nikkiso Switzerland installier. Die Pumpe fördert unter ATEX‐Bedingungen den gelartigen Fertig‐Bulk vom Rührwerk in das Tanklager automatisiert und prozesssicher und ist für die verschiedenen Viskositäten der Medien gut geeignet..
NAMUR‐Technologie‐Roadmap Prozess‐Sensoren 2027+
Authors: Oestreich Volker 
Published: April 2022

AbstractDie Technologie‐Roadmap “Prozess‐Sensoren 2027+” der NAMUR fasst die gemeinsame Technologie‐ und Marktsicht von Anwendern, Herstellern und Forschungseinrichtungen im Bereich Prozesssensorik in der verfahrenstechnischen Industrie zusammen. Digitalisierung und Nachhaltigkeit sind übergreifende Kernthemen der künftigen Entwicklung.
Passend für Ethernet‐APL
Authors: Aust Alexander , Hennecke Andreas 
Published: April 2022

AbstractEthernet‐APL stellt neue Anforderungen an die Gehäusetechnologie für explosionsgefährdeteProzessanlagen. Die Lösung sind zertifizierte Gehäuse, die unterschiedliche Technologien zusammenführen und zu den lokalen, nationalen und internationalen Richtlinien passen.
Bereit für die grüne Energiezukunft
Authors: Manegold Christoph 
Published: April 2022

AbstractUm die anspruchsvolle Prozesstechnik im Elektrolyseur sicher und zuverlässig messen und steuern zu können, braucht es hochpräzise Hochspannungs‐Trennverstärker und Prozessanalytik. Der Beitrag beschreibt die Anforderungen, die an MSR‐Technik in der Wasserstoffgewinnung gestellt werden anhand des Beispiels eines PEM‐Elektrolyseurs eines amerikanischen Herstellers. Hohe Messgenauigkeiten, kurze Einstellzeiten und hohe Spannungsfestigkeit bis in den Kilovoltbereich sind für diese Einsatzzwecke maßgeblich. Desweiteren beschreibt der Beitrag den Einsatz von Sensorik zur Prozessanalyse, um Reinstwasserqualität für die Wasserstoffgewinnung sicherzustellen.