bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Immune Infiltration, Differentially Expressed Genes, and Signaling Pathways in Fanconi Anemia Based on Bioinformatics Analysis
Authors: Shen Biyu , Shi Songsong , Chen Haoyang , Lu Yi , Cui Hengmei , Li Yunyun , Yang Dongliang 
Published: January 2022

Abstract
Background and Objective: Fanconi anemia (FA) patients have a reduced ability to form blood cells, accompanied by multiple congenital malformations, mental retardation, solid tumors, and other symptoms. However, the molecular mechanism that causes FA is unclear, and few studies have addressed the regulatory mechanism of immune infiltration in FA. Here, we aimed to identify differentially expressed genes (DEGs), pathways, and immune infiltration involved in FA using integrated bioinformatics analysis and molecular mechanisms. Methods: The GEO gene chip database was searched for FA low density bone marrow tissue, and the content and proportion of 22 types of immune cells in the FA group and the normal group were analyzed using CIBERSORT. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of FA differentially expressed genes (DEGs) using R language and related package programs was also performed.Results: The expression levels of T cells regulatory (Tregs), M2 macrophages, T cells CD8, dendritic cells resting, and T cells CD4 naïve in FA were higher than in the normal group. Furthermore, the expression levels of naïve B cells, monocytes, and resting mast cells in FA were lower than in the normal group. GO analysis of FA differential genes showed that “neutrophil degranulation,” “neutrophil activation,” and “neutrophil activation involved in immune response,” were most frequently enriched among biological processes, with “specific granule,” “tertiary granule,” “tertiary granule lumen” among cellular components, and “carbohydrate binding” among molecular functions. For the KEGG analysis, “Asthma” was most often enriched.Conclusion: This study obtained useful data related to immune infiltration, DEGs, and gene pathways of FA, and provides new evidence for immunotherapy and clinical assessment of FA patients. These results are potentially a useful reference for subsequent related scientific research.
Combined CTP- IGF-1 Score: A New Score for Assessment of Disease Severity in HCV Related Liver Cirrhosis
Authors: Ghannam Elsayed , Rozaik Salah , Agwa Ramy Hasan , Marwan Ahmed , Mashaly Mervat El-Sayed , Salem Mohamed Mofreh , zekred Abeer Saad El , Saleh Ahmed 
Published: January 2022

Abstract
Background: Liver is the largest endocrine organ in the body. It is a key organ in insulin mediated metabolism, growth hormone and insulin like growth factors (IGF) pathway. Liver cirrhosis is the end result of many chronic diseases including hepatitis C virus infection. Child-Turcotte-Pugh (CTP) score is the standard used in assessment of hepatic reserve but it has its drawbacks in the form of subjective variables, hepatic encephalopathy and ascites. The aim of this work is to assess IGF-1 in patients with liver cirrhosis, correlate it with CTP score and assess value of modified combined CTP-IGF-1 score. Patients and Methods: 170 patients with CLD (liver cirrhosis) and 72 healthy controls in the study groups were enrolled in the study. All patients were subjected to thorough history, clinical examination and laboratory assessment. IGF-1 was measured and all patients were evaluated using CTP and CTP-IGF-1 scores. Results: IGF1 showed highly significant low values in the study group in comparison to controls (42.15 ± 27.976 and 66.31 ± 33.084 ng/ml respectively, p <0.001). It showed also highly significant negative correlation to CTP score in the study group (p < 0.001) with progressive decrements with CTP score stage progression where IGF1 levels were 48.32 ± 28.611, 40.28 ± 25.869 and 18.80 ± 15.953 ng/ml (mean ± standard deviation) in relation to CTP score groups A, B and C respectively (p value < 0.001). The combined CTP-IGF-1 score in comparison to the classic CTP score showed improved area under curve (0.848 and 0.854), sensitivity (71.2% and 88%), negative predictive value (41.7% and 53.7%), false negative results (49 and 19) and accuracy (75.73% and 83.98%) but decreased specificity (97.22% and 61.1%), positive predictive value (99.2% and 91.5%) and higher false positive results (1 and 14) respectively. Conclusion: IGF-1 show progressive decrements with progression of liver cirrhosis and is negatively correlated with CTP score. Addition of IGF-1 to CTP score to formulate combined score improves the AUC, sensitivity, negative predictive value and the accuracy of CTP score and decreases the false negative results.
SARS-CoV-2, alcohol consumption, poor social behaviour, and the COVID-19 pandemic
Authors: Marques Ornelas 
Published: January 2022

Abstract
Studies dealing with the SARS-CoV-2 virus and the COVID-19 disease have concentrated on the study of the virus and the disease themselves, but a knowledge of the causes responsible for the pandemic is still lacking. Crucial for effective decision making and control of the pandemic is a thorough and critical analysis of data regarding COVID-19, which shows an intimate relation between the number of cases/deaths and the consumption of alcohol. Ranked top 30 countries in COVID-19 cases/deaths and alcohol consumption coincide, with only a few exceptions. This coincidence is not fortuitous nor surprising, because excessive alcohol consumption is known to have pernicious effects on social behaviour, i.e. lead to deviant and irresponsible behaviour, which greatly promotes transmission
Open or Not Open the Retroperitoneum: A Pandora’s Box for High-grade Pancreatic Trauma?
Authors: Li Kaiwei , Chen Wensong , Wang Kai , Yang Chao , Deng Yunxuan , Wang Xinyu , Duan Zehua , Chu Chengnan , Hu Yuepeng , Liu Yuxiu , Li Weiqin , Ding Weiwei 
Published: January 2022

Abstract
Background: The management strategy associated with the optimal clinical outcomes for patients with pancreatic trauma remains ambiguous. We sought to determine whether transitioning from initial laparotomy (LAP) to the nonoperative management strategy based on initial percutaneous drainage (PCD) without opening the retroperitoneum would improve clinical outcomes in patients with blunt high-grade pancreatic trauma.Methods: We conducted a retrospective cohort study of pancreatic trauma at a single tertiary referral center. Blunt high-grade pancreatic trauma patients with stable hemodynamics and no diffuse peritonitis were enrolled consecutively in the study. The primary outcome measure was the incidence of severe complications (Clavien-Dindo classification ≥ Ⅲb) for patients who underwent initial LAP vs PCD. To study effect modification by different initial strategies and to adjust for confounding, modified Poisson regression and sensitivity analysis based on propensity score matching and weighting were performed to estimate adjusted relative risks (aRR) and 95% confidence intervals (CIs).Results: Among 119 patients with blunt grade Ⅲ/Ⅳ pancreatic trauma (107 male [89.9%] and 12 female [10.1%]; mean age, 35.7 [SD, 12.7] years), 29 underwent initial PCD and 90 underwent initial LAP (January 2009 through October 2021). Compared with initial LAP, patients underwent initial PCD were significantly lower risk of severe complicates (9/29 [31.0%] vs 65/90 [72.2%]; aRR, 0.52 [95% CI, 0.30-0.90]). Consistent results are also observed in sensitivity analysis models. The relative risk of severe complications for the PCD group in propensity score matching model was 0.53 (95% CI, 0.28-0.99; P = 0.035), 0.37 (95% CI, 0.18-0.75; P = 0.006) in inverse probability of treatment weighting model, and 0.55 (95% CI, 0.31-0.99; P = 0.046) in overlap weighting model. In addition, the mean number of reinterventions per patient was 1.8 in the PCD group and 2.6 in the LAP group (P = 0.067). Conclusions: For blunt high-grade pancreatic trauma patients with stable hemodynamics and no diffuse peritonitis, initial PCD strategy without open the retroperitoneum has a significantly lower rate of severe complications and does not increase reinterventions compared with initial LAP. Further randomized controlled trials are warranted to validate these results.Trial Registration: ClinicalTrials.gov Identifier: NCT03681041(Sept. 21 2018).
Regulation of Life Extension Factor Klotho on Depressive-like Behaviors via Modulation of GluN2B Function in the Nucleus Accumbens
Authors: Wu Han-jun , Liu Liu-er , Wu Wen-ning , Zhan Jin-qiong , Li Yi-heng , Chen Chun-nuan , Jiang Shu-zhen , Xiong Jian-wen , Yu Zhi-Min , Wang Wei , Wei Bo , Yang Yuan-Jian 
Published: January 2022

Abstract
Klotho is a life extension factor that has an ability to regulate the function of GluN2B-containing N-methyl-D-aspartate receptors (NMDARs), whose dysfunction in the nucleus accumbens (NAc) underlies critical aspects of the pathophysiology of major depression. Here we study the functional relevance of klotho in the pathogenesis of depression. A chronic social defeat stress paradigm, where mice are either categorized as susceptible or unsusceptible group based on their performance in a social interaction test, was used in this study. We found that the expression of klotho was largely decreased in the NAc of susceptible mice when compared to control or unsusceptible group. Genetic knockdown of klotho in the NAc induced depressive-like behaviors in naive mice, while overexpression of klotho produced an antidepressive effect in normal mice and ameliorated the depressive-like behaviors in susceptible mice. Molecularly, knockdown of klotho in the NAc resulted in selective decreases of total and synaptic GluN2B expression that were identical to susceptible mice. Elevation of klotho in the NAc reversed the reductions of GluN2B expressions, as well as altered synaptic transmission and spine density in the NAc of susceptible mice. Furthermore, blockade of GluN2B with a specific antagonist abolished the beneficial effects of klotho elevation in susceptible mice. Collectively, we demonstrated that klotho in the NAc modulates depressive-like behaviors by regulating the function of GluN2B-containing NMDARs. These results reveal a novel role for klotho in the pathogenesis of depression, opening new insights into the molecular basis of major depression.
Postural Analysis: Description of a Dedicated System. An Upgrade
Authors: Palandri Sergio 
Published: January 2022

Abstract
BackgroundIn June 2021 an aericle was published describing a possible ssystem to perform postural analysis. In six months use, the ssystem has more clarely showed its lights and shadoes. So the author tought some improvements of the sustem that are now ready to be applyed. This article will show them in details.Materials and MethodsUpgrade interested both aspects close connected to the analysis and both connected to its management .ResultsFirst results obtained are interesting and encourage the use of the upgrades introduced. However a more long period of use is necessary to confirm first resultas.ConclusionsUpgrading is a natural step in every life project. And it is made up not only by a single step. So also this system may be further improved with other upgrades in the future.
Group Penalized Logistic Regressions Predict Ovarian Cancer
Authors: Xie Ying 
Published: January 2022

Abstract
Objectives: Ovarian cancer ranks first among gynecological cancers in terms of the mortality rate. Accurately diagnosing ovarian benign tumors and malignant tumors is of immense important. The goal of this paper is to combine group LASSO/SCAD/MCP penalized logistic regression with machine learning procedure to further improve the prediction accuracy to ovarian benign tumors and malignant tumors prediction problem. Methods: We combine group LASSO/SCAD/MCP penalty with logistic regression, and propose group LASSO/SCAD/MCP penalized logistic regression to predict the benign and malignant ovarian cancer. Firstly, we select 349 ovarian cancer patients data and divide them into two sets: one is the training set for learning, and the other is the testing set for checking, and then choose 46 explanatory variables and divide them into 11 different groups. Secondly, we apply the training set and group coordinate descent algorithm to obtain group LASSO/SCAD/MCP estimator, and apply the testing set to compute confusion matrix, accuracy, sensitivity and specificity. Finally, we compare the prediction performance for group LASSO/SCAD/MCP penalized logistic regression with that for artificial neural network (ANN) and support vector machine (SVM).Results: Group LASSO/SCAD/MCP/ penalized logistic regression selects 6/4/1 groups. The prediction accuracy and AUC for group MCP/SCAD/LASSO penalized logistic regression/SVM/ANN is 93.33%/85.71%/82.26%/74.29%/72.38% and 0.892/0.852/0.823/0.639/0.789, respectively.Conclusions: Group MCP/SCAD/LASSO penalized logistic regression performs than SVM and ANN in terms of prediction accuracy and AUC. In particular, group MCP penalized logistic regression predicts the best. Therefore, we suggest group MCP penalized logistic regression to predict ovarian tumors.
Effects of NRF-1 and PGC-1α Cooperation on HIF-1α and Rat Cardiomyocyte Apoptosis Under Hypoxia
Authors: Niu Nan , Li Hui , Du Xiancai , Wang Chan , Li Junliang , Yang Jihui , Liu Cheng , Yang Songhao , Zhu Yazhou , Zhao Wei 
Published: January 2022

Abstract
Hypoxia is a primary inducer of cardiomyocyte injury, its significant marker being hypoxia-induced cardiomyocyte apoptosis. Nuclear respiratory factor-1 (NRF-1) and hypoxia-inducible factor (HIF)-1α are transcriptional regulatory elements implicated in multiple biological functions, including oxidative stress response. However, their roles in hypoxia-induced cardiomyocyte apoptosis remain unknown. The effect HIF-α, together with NRF-1, exerts on cardiomyocyte apoptosis also remains unclear. We established a myocardial hypoxia model and investigated the effects of these proteins on the proliferation and apoptosis of rat cardiomyocytes (H9C2) under hypoxia. Further, we examined the association between NRF-1 and HIF-1α to improve the current understanding of NRF-1 anti-apoptotic mechanisms. The results showed that NRF-1 and HIF-1α are important anti-apoptotic molecules in H9C2 cells under hypoxia, although their regulatory mechanisms differ. NRF-1 could bind to the promoter region of Hif-1α and negatively regulate its expression. Additionally, HIF-1β exhibited competitive binding with NRF-1 and HIF-1α, demonstrating a synergism between NRF-1 and the peroxisome proliferator-activated receptor-gamma coactivator-1α. These results indicate that cardiomyocytes can regulate different molecular patterns to tolerate hypoxia, providing a novel methodological framework for studying cardiomyocyte apoptosis under hypoxia.
Autophagy Regulates the Effects of Adipose-Derived Stem Cell Small Extracellular Vesicles On Lipopolysaccharide-Induced Pulmonary Microvascular Barrier Damage
Authors: Li Chichi , Wang Min , Wang Wangjia , Li Yuping , Zhang Dan 
Published: January 2022

Abstract
Background: Small extracellular vesicles (sEVs) have been recognized to be more effective than direct stem cell differentiation into functional target cells in preventing tissue injury and promoting tissue repair. Our previous study demonstrated the protective effect of adipose-derived stem cells (ADSCs) on lipopolysaccharide (LPS)-induced acute lung injury and the effect of autophagy on ADSC functions, but the role of ADSC-derived sEVs (ADSC-sEVs) and autophagy-mediated regulation of ADSC-sEVs in LPS-induced pulmonary microvascular barrier damage remains unclear. Methods: After treatment with sEVs from ADSCs with or without autophagy inhibition, LPS-induced human pulmonary microvascular endothelial cell (HPMVECs) barrier damage was detected. LPS-induced acute lung injury in mice was assessed in vivo after intravenous administration of sEVs from ADSCs with or without autophagy inhibition. The effects of autophagy on the bioactive miRNA components of ADSC-sEVs were assessed after prior inhibition of cell autophagy. Results: We found that ADSC-sEV effectively alleviated LPS-induced apoptosis, tight junction damage and high permeability of PMVECs. Moreover, in vivo administration of ADSC-sEV markedly inhibited LPS-triggered lung injury. However, autophagy inhibition, markedly weakened the therapeutic effect of ADSC-sEVs on LPS-induced PMVECs barrier damage and acute lung injury. In addition, autophagy inhibition, prohibited the expression of five specific miRNAs in ADSC-sEVs -under LPS-induced inflammatory conditions. Conclusions: Our results indicate that ADSC-sEVs protect against LPS-induced pulmonary microvascular barrier damage and acute lung injury. Autophagy is a positive mediator of sEVs function, at least in part through controlling the expression of bioactive miRNAs in sEVs.
Urban Development Reduces Bee Abundance and Diversity
Authors: Pfeiffer Vera Wilder , Crowder David W. , Silbernagel Janet 
Published: January 2022

Abstract
Wild bee communities persist in cities despite major disruption of nesting and food resources by urban development. Bee diversity and abundance is key for urban agriculture and maintenance of plant diversity, and assessing what aspects of cities enhance bee populations will promote our capacity to retain and provision bee habitat. Here, we assessed how variation in land cover and neighborhood development history affected bee communities in the midwestern US urban landscape of Madison, Wisconsin. We sampled bee communities across 38 sites with relatively high (> 55%) or low (< 30%) levels of impervious surface, and assessed effects of land use and neighborhood development history on bee abundance and species richness. We show abundance and richness of bees was lower in recently developed neighborhoods, with particularly strong negative effects on soil nesting bees. Soil nesting bees and bee community richness decreased as cover of impervious surface increased, but above ground nesting bees were minimally impacted. Bee community similarity varied spatially and based on dissimilar local land cover, only for soil nesting bees, and the overall bee community. Impervious surface limited bee abundance and diversity, but new neighborhoods were associated with greater negative effects. We suggest that enhancing the structural diversity of new neighborhoods in urban ecosystems may imitate the structural benefits of older neighborhoods for bee populations.
An Experimental Study on the Effect of Micro-Metakaolin on the Strength and Swelling Characteristics of Expansive Soils
Authors: Sakr Mohamed , Azzam Waseim , Meguid Mohamed , Ghoneim Hebatalla 
Published: January 2022

Abstract

Expansive soils are found in many parts of the world, especially in arid areas and dry weather regions. Urbanization and development of new cities around the world resulted in construction in areas of challenging subsurface soil conditions. For example, in the Middle East, the Government of Egypt is building several new cities to accommodate the continuous increase in the country’s population. Most of these new cities are located in areas underlain by expansive soils. In this study, a series of laboratory tests were carried out to investigate the effect of introducing micro-metakaolin into the matrix of an expansive soil to improve the swelling potential as a new stabilizing material. Test results showed that micro-metakaolin can considerably decrease the free swell index of the soil by 37% and 54% at micro-metakaolin content of 15% and 25%, respectively. In addition, the shear strength of the soil was found to also increase as a result of the introduction of the micro-metakaolin material. Adding 25% micro-metakaolin content reduced the swelling pressure of the soil by about 33%. The results suggest that the proposed method is efficient in stabilizing and improving the properties of expansive soils found in arid areas. This is important to control excessive swelling and prevent possible damage to the supported structures.
Does Prevalence of Sexual Dysfunction Differ Among the Most Common Causes of Infertility? A Cross-Sectional Study
Authors: Ashrafi Mahnaz , Jahangiri Nadia , Sadatmahalleh Shahideh Jahanian , Mirzaei Negin , Hesari Naiiere Gharagozloo , Rostami Frahnaz , Mousavi Seyedeh Saeedeh , Zeinaloo Mona 
Published: January 2022

Abstract
Background

Sexuality as a fundamental component of women’s health, can be affected by infertility. The current study aimed at comparing the prevalence of sexual dysfunction among women with the most common causes of infertility.
Methods

The current cross-sectional study was conducted on 240 infertile females with infertility due to polycystic ovary syndrome (PCOS, n=80), endometriosis (n=80) and male factor (n=80) at Royan Institute for Reproductive Biomedicine (Tehran, Iran) and 160 fertile women at health care centers, between May 2016 and June 2017. Sexual function was assessed by Female Sexual Function Index (FSFI). Data were analyzed using SPSS (version 25.00) and differences were regarded statistically significant at p < 0. 05.
Results

The prevalence of female sexual dysfunction was 98.8% in women with PCOS, 100.0% in those with endometriosis, and 80.0% in those with male factor infertility. Overall, 36.2% of the enrolled fertile women were suffering from sexual dysfunction.
Conclusions

There was an association between the prevalence of female sexual dysfunction or individual domain scores of the FSFI, and infertility etiologies. Therefore, infertility care providers are required to take this into consideration and develop preventive strategies in this regard.
Factors influencing and changes in childhood vaccination coverage over time in Bangladesh: A multilevel mixed-effects analysis
Authors: Kundu Satyajit , Kundu Subarna , Seidu Abdul-Aziz , Okyere Joshua , Ghosh Susmita , Hossain Ahmed , Alshahrani Najim Z. , Banna Md. Hasan Al , Rahman Md. Ashfikur , Ahinkorah Bright Opoku 
Published: January 2022

Abstract
There is a dearth of information on childhood vaccination coverage in Bangladesh. Thus, this study aimed to investigate the associated factors and changes in childhood vaccination coverage over time in Bangladesh. Bangladesh's Demographic and Health Surveys from 2011, 2014, and 2017-18 provided data on vaccination coverage for children aged 12 to 35 months. For three survey periods, multilevel binary logistic regression models were employed. The overall prevalence (weighted) of full vaccination among children aged 12-35 months was 86.17% in 2011 and 85.13% in 2014, and 89.23% in 2017-18. Children from families with high wealth index, mothers with higher education, and over the age of 24 and who sought at least four ANC visits, as well as children from urban areas were more likely to receive full vaccination. Rangpur division had the highest change rate of immunization coverage from 2011 to 2014 (2.26%), whereas Sylhet division had the highest change rate from 2014 to 2017-18 (34.34%). To improve immunization coverage for Bangladeshi children, policymakers must integrate vaccine programs, paying special attention to mothers without at least a high school education and families with low wealth index. Increased antenatal care visits may also aid in increasing the immunization coverage of their children.