bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatially restricted drivers and transitional cell populations cooperate with the microenvironment in untreated and chemo-resistant pancreatic cancer
Authors: Cui Zhou Daniel , Jayasinghe Reyka G. , Chen Siqi , Herndon John M. , Iglesia Michael D. , Navale Pooja , Wendl Michael C. , Caravan Wagma , Sato Kazuhito , Storrs Erik , Mo Chia-Kuei , Liu Jingxian , Southard-Smith Austin N. , Wu Yige , Naser Al Deen Nataly , Baer John M. , Fulton Robert S. , Wyczalkowski Matthew A. , Liu Ruiyang , Fronick Catrina C. , Fulton Lucinda A. , Shinkle Andrew , Thammavong Lisa , Zhu Houxiang , Sun Hua , Wang Liang-Bo , Li Yize , Zuo Chong , McMichael Joshua F. , Davies Sherri R. , Appelbaum Elizabeth L. , Robbins Keenan J. , Chasnoff Sara E. , Yang Xiaolu , Reeb Ashley N. , Oh Clara , Serasanambati Mamatha , Lal Preet , Varghese Rajees , Mashl Jay R. , Ponce Jennifer , Terekhanova Nadezhda V. , Yao Lijun , Wang Fang , Chen Lijun , Schnaubelt Michael , Lu Rita Jui-Hsien , Schwarz Julie K. , Puram Sidharth V. , Kim Albert H. , Song Sheng-Kwei , Shoghi Kooresh I. , Lau Ken S. , Ju Tao , Chen Ken , Chatterjee Deyali , Hawkins William G. , Zhang Hui , Achilefu Samuel , Chheda Milan G. , Oh Stephen T. , Gillanders William E. , Chen Feng , DeNardo David G. , Fields Ryan C. , Ding Li 
Published: August 2022

AbstractPancreatic ductal adenocarcinoma is a lethal disease with limited treatment options and poor survival. We studied 83 spatial samples from 31 patients (11 treatment-naïve and 20 treated) using single-cell/nucleus RNA sequencing, bulk-proteogenomics, spatial transcriptomics and cellular imaging. Subpopulations of tumor cells exhibited signatures of proliferation, KRAS signaling, cell stress and epithelial-to-mesenchymal transition. Mapping mutations and copy number events distinguished tumor populations from normal and transitional cells, including acinar-to-ductal metaplasia and pancreatic intraepithelial neoplasia. Pathology-assisted deconvolution of spatial transcriptomic data identified tumor and transitional subpopulations with distinct histological features. We showed coordinated expression of TIGIT in exhausted and regulatory T cells and Nectin in tumor cells. Chemo-resistant samples contain a threefold enrichment of inflammatory cancer-associated fibroblasts that upregulate metallothioneins. Our study reveals a deeper understanding of the intricate substructure of pancreatic ductal adenocarcinoma tumors that could help improve therapy for patients with this disease.
Exploring phytochemical composition, photocatalytic, antibacterial, and antifungal efficacies of Au NPs supported by Cymbopogon flexuosus essential oil
Authors: Pathania Diksha , Sharma Mamta , Thakur Pankaj , Chaudhary Vishal , Kaushik Ajeet , Furukawa Hidemitsu , Khosla Ajit 
Published: August 2022

AbstractBiofabrication of gold nanoparticles (AuNPs) using the aromatic essential oils is highlighted due to its simple, economical, low toxicity, and eco-friendly nature. Essential oil of Cymbopogon flexuosus (CF), an economically valuable medicinal plant, exhibits anti-inflammatory, anti-tumor, antioxidant, and antimicrobial activities. For the first time, this research accounts for the biosynthesis, physicochemical, photocatalytic, antifungal, antibacterial properties of biogenic AuNPs, fabricated using CF essential oil collected from different altitudes (S1-Palampur, S2-Haryana, S3-Dehradun). The altitudinal disparity in the phytochemical composition of essential oils is highlighted. The average crystallite size ranged from 10 to 32 nm and was influenced by CF samples used in the synthesis. The spectroscopic outcomes revealed the involvement of bioactive reagents from CF essential oil in the fabrication and stabilization of AuNPs. The fabricated AuNPs exhibited excellent antimicrobial activities against all tested strains (Staphyloccucs aureus, Escherichia coli, Fusarium oxysporum), showing their efficacy as an antimicrobial agent to treat infectious diseases. Moreover, AuNPs exhibited excellent photocatalytic efficacy of around 91.8% for the degradation of methylene blue under exposure of direct sunlight for 3 h without the assistance of an external reducing agent. The outcomes highlight a potential economic and environmentally friendly strategy to fabricate biogenic AuNPs for diversified industrial applications where antimicrobial and photocatalytic efficacies are the key requirements.
Multifactor assessments to determine the overall performance of supercritical fluid extraction from Gynura procumbens essential oil
Authors: Idris Sitinoor Adeib , Markom Masturah , Abd. Rahman Norliza , Mohd Ali Jarinah 
Published: August 2022

AbstractGynura procumbens is a medicinal herb that contains bioactive compounds that can relieve coughs and prevent liver cancer. Supercritical fluid extraction (SFE) was suggested as one of the techniques that can be used to extract the valuable compounds from the G. procumbens. SFE was widely applied in extracting medicinal ingredients from herbs. However, most of them were performed only at the laboratory scale. Moreover, study to increase the yield performance, economic studies and safety assessments of the SFE process were also performed; however, these tests were conducted individually. Moreover, to date, there is no integration study between all the factors stated for determining the overall performance of SFE with herbs specifically G. procumbens. The integration between all the factors is beneficial because the data on the overall performance can assist in developing the SFE process with G. procumbens at the pilot or industrial scale. Therefore, this study incorporated a multifactor approach to measure the overall performance of the SFE process towards G. procumbens by using a rating and index approach. A summary of factors, such as the solubility of G. procumbens in CO2, operational cost and safety assessment elements, were taken into consideration as the main influences that determine the overall performance index of this study. Iperformance or overall performance of SFE from G. procumbens was successfully assessed and compared with response surface methodology (RSM). Overall, the results from Iperformance exhibit satisfactory solubility values when compared to the optimized value from RSM when considering the lowest operational costs in the safest SFE environment.
A single latent channel is sufficient for biomedical glottis segmentation
Authors: Kist Andreas M. , Breininger Katharina , Dörrich Marion , Dürr Stephan , Schützenberger Anne , Semmler Marion 
Published: August 2022

AbstractGlottis segmentation is a crucial step to quantify endoscopic footage in laryngeal high-speed videoendoscopy. Recent advances in deep neural networks for glottis segmentation allow for a fully automatic workflow. However, exact knowledge of integral parts of these deep segmentation networks remains unknown, and understanding the inner workings is crucial for acceptance in clinical practice. Here, we show that a single latent channel as a bottleneck layer is sufficient for glottal area segmentation using systematic ablations. We further demonstrate that the latent space is an abstraction of the glottal area segmentation relying on three spatially defined pixel subtypes allowing for a transparent interpretation. We further provide evidence that the latent space is highly correlated with the glottal area waveform, can be encoded with four bits, and decoded using lean decoders while maintaining a high reconstruction accuracy. Our findings suggest that glottis segmentation is a task that can be highly optimized to gain very efficient and explainable deep neural networks, important for application in the clinic. In the future, we believe that online deep learning-assisted monitoring is a game-changer in laryngeal examinations.
Abiotic and biotic factors controlling the dynamics of soil respiration in a coastal dune ecosystem in western Japan
Authors: Teramoto Munemasa , Hamamoto Toru , Liang Naishen , Taniguchi Takeshi , Ito Takehiko Y. , Hu Richa , Yamanaka Norikazu 
Published: August 2022

AbstractIn this study, we examined the abiotic and biotic factors controlling the dynamics of soil respiration (Rs) while considering the zonal distribution of plant species in a coastal dune ecosystem in western Japan, based on periodic Rs data and continuous environmental data. We set four measurement plots with different vegetation compositions: plot 1 on bare sand; plot 2 on a cluster of young Vitex rotundifolia seedlings; plot 3 on a mixture of Artemisia capillaris and V. rotundifolia; and plot 4 on the inland boundary between the coastal vegetation zone and a Pinus thunbergii forest. Rs increased exponentially along with the seasonal rise in soil temperature, but summer drought stress markedly decreased Rs in plots 3 and 4. There was a significant positive correlation between the natural logarithm of belowground plant biomass and Rs in autumn. Our findings indicate that the seasonal dynamics of Rs in this coastal dune ecosystem are controlled by abiotic factors (soil temperature and soil moisture), but the response of Rs to drought stress in summer varied among plots that differed in dominant vegetation species. Our findings also indicated that the spatial dynamics of Rs are mainly controlled by the distribution of belowground plant biomass and autotrophic respiration.
Human health implications of trace metal contamination in topsoils and brinjal fruits harvested from a famous brinjal-producing area in Bangladesh
Authors: Bushra Anika , Zakir H. M. , Sharmin Shaila , Quadir Q. F. , Rashid M. H. , Rahman M. S. , Mallick Supti 
Published: August 2022

AbstractA study was undertaken to determine the contents of trace metals in 60 topsoils and 80 brinjal fruits samples from a famous brinjal-producing area of Bangladesh using atomic absorption spectrophotometer. The study also looked at soil pollution levels, dietary intake of nutritionally important trace elements, and human health risks from toxic metals induced by dermal soil exposure and consumption of brinjal. The content of Pb, Ni, Cd, Cu, Fe, Mn, and Zn in brinjal fruits harvested from farmer′s fields ranged from 0.204–0.729, 0.031–0.212, < 0.010–0.061, 1.819–2.668, 3.267–5.910, < 0.010–0.866 and 2.160–3.846 µg g-1, respectively, while the amount of Cr was negligible. The calculated enrichment factors showed that 70, 50, and 25% of soil sampling sites had values in the 2.00–5.00 range for Pb, Zn, and Cd, respectively, while 30% of sites had values > 5.00 for Cd, indicating moderate to significant enrichment of these metals in the soil. The study also revealed that brinjal consumption provides a tiny amount of nutritionally important trace elements required for an adult human. Regarding the computed incremental lifetime cancer risks (ILCR), the study revealed that the values for Pb and Ni in all samples and Cd in 40% of samples were several hundred times higher for males and females than the USEPA threshold level due to oral ingestion of brinjal fruits. In contrast, dermal exposures to soil trace elements were within an acceptable range. The PCA results revealed that the contents of Cd, Pb, Ni, and Cu in soils showed strong positive correlations with those elements present in brinjal. The current study suggests future traceability research, focusing on pinpointing potential entry routes for toxic elements into the vegetable food chain.
The relationship between just world belief and wellbeing, cheating behaviors, and academic work behaviors during COVID 19 among university students
Authors: Münscher Susan 
Published: August 2022

AbstractIs the belief in a just world among students also stable under COVID-19? To answer this question, a study was conducted with university students from Germany (n = 291). The aim of the study was to analyze the predictive performance of the personal belief in a just world (PBJW) on students' life satisfaction and academic cheating and to take into account important mediators from the university context such as fellow student justice, lecturer justice, and procrastination. Derived from existing research, university students with a stronger PBJW should be more satisfied with their lives and cheat less than those with a weaker PBJW. The results support the hypothesized direct effects of PBJW on life satisfaction. Procrastination additionally mediated the effect of PBJW on life satisfaction. The level of PBJW predicted academic cheating only indirectly. The mediators procrastination and lecturer justice were crucial here. The results persisted when gender, learning, time to exam, socially desirable responding, general BJW, and self-efficacy were controlled. The findings were discussed in relation to the stressful situation caused by COVID-19. A reflection on the adaptive function of PBJW as a resource and relevant situation-specific mediators for university research and practice followed.
Color image encryption scheme based on alternate quantum walk and controlled Rubik’s Cube
Authors: Zhao Jingbo , Zhang Tian , Jiang Jianwei , Fang Tong , Ma Hongyang 
Published: August 2022

AbstractAiming at solving the trouble that digital image information is easily intercepted and tampered during transmission, we proposed a color image encryption scheme based on alternate quantum random walk and controlled Rubik’s Cube transformation. At the first, the color image is separated into three channels: channel R, channel G and channel B. Besides, a random sequence is generated by alternate quantum walk. Then the six faces of the Rubik’s Cube are decomposed and arranged in a specific order on a two-dimensional plane, and each pixel of the image is randomly mapped to the Rubik’s Cube. The whirling of the Rubik’s Cube is controlled by a random sequence to realize image scrambling and encryption. The scrambled image acquired by Rubik’s Cube whirling and the random sequence received by alternate quantum walk are bitwise-XORed to obtain a single-channel encrypted image. Finally the three-channel image is merged to acquire the final encrypted image. The decryption procedure is the reverse procedure of the encryption procedure. The key space of this scheme is theoretically infinite. After simulation experiments, the information entropy after encryption reaches 7.999, the NPCR is 99.5978%, and the UACI is 33.4317%. The encryption scheme with high robustness and security has a excellent encryption effect which is effective to resist statistical attacks, force attacks, and other differential attacks.
Carbon nanoparticles adversely affect CFTR expression and toxicologically relevant pathways
Authors: Stermann Torben , Nguyen Thach , Stahlmecke Burkhard , Todea Ana Maria , Woeste Selina , Hacheney Inken , Krutmann Jean , Unfried Klaus , Schins Roel P. F. , Rossi Andrea 
Published: August 2022

AbstractCystic fibrosis is an autosomal recessive disorder caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) that can lead to terminal respiratory failure. Ultrafine carbonaceous particles, which are ubiquitous in ambient urban and indoor air, are increasingly considered as major contributors to the global health burden of air pollution. However, their effects on the expression of CFTR and associated genes in lung epithelial cells have not yet been investigated. We therefore evaluated the effects of carbon nanoparticles (CNP), generated by spark-ablation, on the human bronchial epithelial cell line 16HBE14o− at air–liquid interface (ALI) culture conditions. The ALI-cultured cells exhibited epithelial barrier integrity and increased CFTR expression. Following a 4-h exposure to CNP, the cells exhibited a decreased barrier integrity, as well as decreased expression of CFTR transcript and protein levels. Furthermore, transcriptomic analysis revealed that the CNP-exposed cells showed signs of oxidative stress, apoptosis and DNA damage. In conclusion, this study describes spark-ablated carbon nanoparticles in a realistic exposure of aerosols to decrease CFTR expression accompanied by transcriptomic signs of oxidative stress, apoptosis and DNA damage.
Field validation of deep learning based Point-of-Care device for early detection of oral malignant and potentially malignant disorders
Authors: Birur N. Praveen , Song Bofan , Sunny Sumsum P. , G. Keerthi , Mendonca Pramila , Mukhia Nirza , Li Shaobai , Patrick Sanjana , G. Shubha , A.R. Subhashini , Imchen Tsusennaro , Leivon Shirley T. , Kolur Trupti , Shetty Vivek , R. Vidya Bhushan , Vaibhavi Daksha , Rajeev Surya , Pednekar Sneha , Banik Ankita Dutta , Ramesh Rohan Michael , Pillai Vijay , O.S. Kathryn , Smith Petra Wilder , Sigamani Alben , Suresh Amritha , Liang Rongguang , Kuriakose Moni A. 
Published: August 2022

AbstractEarly detection of oral cancer in low-resource settings necessitates a Point-of-Care screening tool that empowers Frontline-Health-Workers (FHW). This study was conducted to validate the accuracy of Convolutional-Neural-Network (CNN) enabled m(mobile)-Health device deployed with FHWs for delineation of suspicious oral lesions (malignant/potentially-malignant disorders). The effectiveness of the device was tested in tertiary-care hospitals and low-resource settings in India. The subjects were screened independently, either by FHWs alone or along with specialists. All the subjects were also remotely evaluated by oral cancer specialist/s. The program screened 5025 subjects (Images: 32,128) with 95% (n = 4728) having telediagnosis. Among the 16% (n = 752) assessed by onsite specialists, 20% (n = 102) underwent biopsy. Simple and complex CNN were integrated into the mobile phone and cloud respectively. The onsite specialist diagnosis showed a high sensitivity (94%), when compared to histology, while telediagnosis showed high accuracy in comparison with onsite specialists (sensitivity: 95%; specificity: 84%). FHWs, however, when compared with telediagnosis, identified suspicious lesions with less sensitivity (60%). Phone integrated, CNN (MobileNet) accurately delineated lesions (n = 1416; sensitivity: 82%) and Cloud-based CNN (VGG19) had higher accuracy (sensitivity: 87%) with tele-diagnosis as reference standard. The results of the study suggest that an automated mHealth-enabled, dual-image system is a useful triaging tool and empowers FHWs for oral cancer screening in low-resource settings.
Estimation of porcine pancreas optical properties in the 600–1100 nm wavelength range for light-based therapies
Authors: Lanka Pranav , Bianchi Leonardo , Farina Andrea , De Landro Martina , Pifferi Antonio , Saccomandi Paola 
Published: August 2022

AbstractThis work reports the optical properties of porcine pancreatic tissue in the broad wavelength range of 600–1100 nm. Absorption and reduced scattering coefficients (µa and µs′) of the ex vivo pancreas were obtained by means of Time-domain Diffuse Optical Spectroscopy. We have investigated different experimental conditions—including compression, repositioning, spatial sampling, temporal stability—the effect of the freezing procedure (fresh vs frozen-thawed pancreas), and finally inter-sample variability. Good repeatability under different experimental conditions was obtained (median coefficient of variation less than 8% and ~ 16% for µa and µs′, respectively). Freezing–thawing the samples caused an irreversible threefold reduction of µs′ and no effect on µa. The absorption and reduced scattering spectra averaged over different samples were in the range of 0.12–0.74 cm−1 and 12–21 cm−1 with an inter-sample variation of ~ 10% and ~ 40% for µa and µs′, respectively. The calculated effective transport coefficient (µeff) for fresh pancreatic tissue shows that regions between 800–900 nm and 1050–1100 nm are similar and offer the lowest tissue attenuation in the considered range (i.e., µeff ranging from 2.4 to 2.7 cm−1). These data, describing specific light-pancreas interactions in the therapeutic optical window for the first time, provide pivotal information for planning of light-based thermotherapies (e.g., laser ablation) and instruction of light transport models for biophotonic applications involving this organ.
Voltammetric estimation of residual nitroxynil in food products using carbon paste electrode
Authors: Salim Mohamed M. , Ashraf Sally , Hashem Heba M. , Belal Fathalla 
Published: August 2022

AbstractA simple and sensitive voltammetric method was developed and validated for the recognition of the veterinary drug nitroxynil (NTX). The method is based on studying its voltammetric behavior at a carbon paste electrode. Square wave voltammetry (SWV) was successfully applied in this study. The anodic peak current obtained was a linear function of NTX concentration in Britton Robinson buffer of pH 3 over the range of 3.9 × 10–6–1.0 × 10−4 M with lower detection and quantitation limits of 3.1 × 10–7and 9.4 × 10–7 M, respectively. The proposed method was first applied to the assessment of the drug in commercial vials. The method was further used to monitor the residual amounts of the drug in bovine meat, kidney, fat, and milk samples. The results obtained were favourably compared with those given by reference method. The interference likely to be introduced by co-administered drugs was evaluated. The electrode reaction was elucidated, and electron transfer kinetics were studied.
Assessment of creatinine concentration in whole blood spheroids using paper spray ionization–tandem mass spectrometry
Authors: Sham Tung-Ting , Badu-Tawiah Abraham K. , McWilliam Stephen J. , Maher Simon 
Published: August 2022

AbstractAccurate quantification of blood creatinine is important to estimate the glomerular filtration rate. Existing techniques using liquid chromatography tandem mass spectrometry (LC–MS/MS) have a high accuracy and eliminate most interferences encountered in routine enzymatic and Jaffé methods. However, they require laborious and time-consuming sample treatment and data acquisition. The aim of this study is to develop a fast and simple method to enable a direct analysis of whole blood creatinine with performance measures that are comparable to conventional LC–MS/MS. 5μL whole blood is formed as a three-dimensional spheroid on hydrophobic silanized paper substrates which then undergoes paper-spray ionization—tandem mass spectrometry (PSI–MS/MS). The method is validated using real human samples and compared with LC–MS/MS. PSI–MS/MS whole blood analysis exhibited a lower limit of quantification of 2.5 μg/mL, precision ≤ 6.3%, recovery in the range of 88–94% and excellent linearity (R2 > 0.99; 2.5—20 μg/mL) covering the normal range for creatinine levels. Creatinine levels were comparable to those measured by LC–MS/MS with small deviations of less than 0.3 μg/mL. This simple, fast and accurate microsampling technique for direct analysis of creatinine from whole blood shows promise for routine clinical screening and monitoring. This approach can be readily extended for other analytes of interest and, due to inherent advantages relating to cost, storability, speed, and simplicity, it can be especially advantageous for use in resource-limited settings.