bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enterovibrio paralichthyis sp. nov., isolated from the gut of an olive flounder Paralichthys olivaceus
Authors: Kim Young-Ok , Park In-Suk , Kim Dong-Gyun , Park Sooyeon , Han Sung Hoon , Yoon Jung-Hoon 
Published: December 2022

A Gram-stain-negative, facultatively anaerobic, flagellated and coccoid, ovoid or rod-shaped bacterial strain, NIFS-20-8T, was isolated from the intestine of an olive flounder (Paralichthys olivaceus) from the East Sea, Republic of Korea. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain NIFS-20-8T fell within the clade comprising the type strains of

Enterovibrio

species. Strain NIFS-20-8T exhibited 16S rRNA gene sequence similarities of 97.2 and 97.1 % to the type strains of

Enterovibrio nigricans

and

Enterovibrio norvegicus

, respectively, and of 96.6–97.0 % to the type strains of the other

Enterovibrio

species. The average nucleotide identity and digital DNA–DNA hybridization values between the genomic sequence of strain NIFS-20-8T and those of the type strains of four

Enterovibrio

species were 73.8–75.0 and 19.8–21.1 %, respectively. The DNA G+C content of strain NIFS-20-8T from genomic sequence data was 50.55 mol%. Strain NIFS-20-8T contained Q-8 as the predominant ubiquinone and summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c), C16 : 0 and C18 : 1
ω7c as the major fatty acids. The major polar lipids detected in stain NIFS-20-8T were phosphatidylethanolamine and phosphatidylglycerol. Distinguishing phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain NIFS-20-8T is separated from recognized

Enterovibrio

species. On the basis of the data presented here, strain NIFS-20-8T is considered to represent a novel species of the genus

Enterovibrio

, for which the name Enterovibrio paralichthyis sp. nov. is proposed. The type strain is NIFS-20-8T (= KCTC 82873T=NBRC 115237T).
Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain
Authors: Lin Ya-Hui , Wang Li-Wen , Chen Yen-Hui , Chan Yi-Chieh , Hu Shang-Hsiu , Wu Sheng-Yan , Chiang Chi-Shiun , Huang Guan-Jie , Yang Shang-Da , Chu Shi-Wei , Wang Kuo-Chuan , Lin Chin-Hsien , Huang Pei-Hsin , Cheng Hwai-Jong , Chen Bi-Chang , Chu Li-An 
Published: December 2022

AbstractTissue clearing and labeling techniques have revolutionized brain-wide imaging and analysis, yet their application to clinical formalin-fixed paraffin-embedded (FFPE) blocks remains challenging. We introduce HIF-Clear, a novel method for efficiently clearing and labeling centimeter-thick FFPE specimens using elevated temperature and concentrated detergents. HIF-Clear with multi-round immunolabeling reveals neuron circuitry regulating multiple neurotransmitter systems in a whole FFPE mouse brain, and is able to be used as the evaluation of disease treatment efficiency. HIF-Clear also supports expansion microscopy and can be performed on a non-sectioned 15-year-old FFPE specimen, as well as a 3-month formalin-fixed mouse brain. Thus, HIF-Clear represents a feasible approach for researching archived FFPE specimens for future neuroscientific and 3D neuropathological analyses.
Clinical impact using low dose mycophenolate mofetil with tacrolimus on acute rejection, infectious diseases and non-infectious complications in renal transplant: A Single Hospital Experience in Mexico
Authors: Andrade-Sierra Jorge , Hernández-Reyes Hernesto , Rojas-Campos Enrique , Cardona-Muñoz Ernesto G , Cerrillos-Gutiérrez José I , González-Espinoza Eduardo , Evangelista-Carrillo Luis A , Medina-Pérez Miguel , Jalomo-Martínez Basilio , Miranda-Díaz Alejandra G , Martínez-Mejía Víctor M , Gómez-Navarro Benjamin , Andrade-Ortega Antonio de Jesús , Nieves-Hernández Juan J , Mendoza-Cerpa Claudia 
Published: December 2022

AbstractBackgroundThe evidence supporting a starting dose of 2 g/day of mycophenolate mofetil (MMF) in combination with tacrolimus (TAC) in renal transplant (RT) is still limited but maintaining the dose of less than 2g could result in worse clinical outcomes in terms of acute rejection (AR).Research QuestionThis study’s aim was to determine the association between AR, infectious and non-infectious complications after RT with the dose of 1.5gvs. 2g of MMF.DesignA prospective cohort was performed with a 12-month follow-up in recipients of RT from living donors with low doses of MMF (1.5 g/day) or use of standard dosage (2 g/day). The association between adverse effects and complications and doses of MMF were examined in Cox proportional hazard models and survival free of AR, infectious diseases, and non-infectious complications was evaluated by the Kaplan-Meier test.ResultsAt the end of the follow-up, the incidence of infectious diseases was 52%vs. 50% (p=0.71) and AR was 5%vs. 5% (p=0.86), respectively. Survival free of GI complications requiring medical attention was higher in the low dose group compared to the standard dose (88%vs. 45%, respectively;p<0.001).DiscussionThe use of 1.5 g/day of MMF is safe in Mexican population with RT from living donors without increasing the risk of AR, and it confers a reduction of adverse events.
Knowledge, Attitude and Practice (KAP) Survey about hepatitis B (HBV) and C (HCV) among migrant populations from Sub-Saharan Africa
Authors: Saaed Frhat M. A. , Ongerth Jerry E. , Ali Muna H. 
Published: December 2022

AbstractBackgroundHepatitis B and C virus infections are serious blood borne diseases with global health concern particularly in developing countries. The association between survey participant’s knowledge, attitude, and practices (KAP) about HBV and HCV infection is rarely studied, particularly among migrants seeking resettlement. Libya is a main transient station for migrants northward toward Europe and the flow is increasing into Al Kufra from neighboring countries that have endemic intermediate and high HBV and HCV prevalence. The purpose of this exploratory descriptive study in Al Kufra was to assess the level of participating migrant’s KAP, regarding cause, transmission, prevention, and treatment of viral hepatitis due to HBV and HCV.MethodA cross-sectional study was conducted among 674 sub-Saharan African migrants in Al Kufra, Libya from January to October 2021. Migrant’s KAP about HBV and HCV infection was assessed by interview questionnaire. Statistical and data analysis used SPSS version of 25.ResultsOut of 700 questionnaires distributed, 674 were returned with a response rate of 96.3%. The study population included 580 (86.1%) males, mean age of 29.6 ± 7.2 SD years. A majority, 508 (75.4%) were illiterate and elementary level, 8 2.483+.232, respectively. Positive significant linear correlations were found between knowledge-attitude (r = 0.524, p < 0.01) knowledge-practice (r = 0.123, p < 0.01) and attitude-practice (r = 0.278, p < 0.01). Ethnicity and education variables were significantly associated with mean KAP. Gender identity and marital status were both significantly associated with mean knowledge and attitude.ConclusionThe migrant population from the neighboring and sub-Saharan African countries have inadequate KAP about HBV and HCV to limit infection.
Centrosome formation in the bovine early embryo
Authors: Uzbekov Rustem , Singina Galina , Shedova Ekaterina , Banliat Charles , Avidor-Reiss Tomer , Uzbekova Svetlana 
Published: December 2022

SummaryCentrosome formation during early development in mice and rats occurs due to the appearance of centriolesde novo. In contrast, in humans and other non-rodent mammals, centrioles are thought to be derived from spermatozoa. Ultrastructural study of zygotes and early embryos of cattle at full series of ultrathin sections showed that the proximal centriole of the spermatozoon disappears by the end of the first cleavage division. Centrioles appear in 2–4 cell embryos in fertilized oocytes and in parthenogenetic embryos. Centriole formation includes the appearance of atypical centrioles with randomly arranged triplets and centrioles with microtubule triplets of various lengths. After the third cleavage, four centriolar cylinders for the first time appear in the blastomeres while each embryo still has two atypical centrioles. Our results showed that the mechanisms of centriole formation in different groups of mammals are universal, differing only in the stage of development in which they occur.
A Novel Localized Tracing Technique to Explore Intra-Amygdala Functional and Structural Connectivity Patterns as Mediators of Individual Variability in Stress Response
Authors: Lipshutz Allie , Saltz Victoria , Anderson Kristin R , Manganaro Alessia , Dumitriu Dani 
Published: December 2022

ABSTRACTNeuropsychiatric disorders including anxiety and depression can be induced by stress, but not all individuals exposed to stress develop psychopathology. Therefore, probing the neural substrates that underlie trait vulnerability to stress may open the door for preventive approaches that use biological markers to identify at-risk populations. Here, we developed a novel tracing technique to probe local connectivity patterns as predictors of individual variability in stress responses. Specifically, we combined a retrograde transsynaptic rabies tracing system with cFos colocalization immediately after an acute stressor to elucidate local structural and stress-activated (functional) differences in connectivity within the amygdala complex in female and male mice along a spectrum of social approach/avoidance following acute social defeat stress. While we find no structural or functional connections within the amygdala complex as predictors of individual variability in our behavioral readout, our methods provide a novel approach to investigating cellular and behavioral individual variability in stress responses. Furthermore, we identify overall stress-activation as a predictor of social approach/avoidance in two subregions, medial amygdala and piriform-amygdala area, which may serve as potential biological markers of trait vulnerability, with possible clinical applicability.Significance StatementExposure to stress is ubiquitous, but the outcomes of stress exposure vary greatly between individuals. Our work introduces a novel tracing technique to probe for structural and functional connectivity patterns across a broad spectrum of behavioral responses to social stress. Utilizing pre-clinical classification in conjunction with representative behavioral classification introduces to the field a mechanism to identify potential clinical targets for preventative screening for neuropsychiatric disorders as well as further individualized treatment. Notably, our work identifies two intra-amygdalar neural correlates of social stress, opening the door for future investigation of the role these regions play in mediating social stress.
Data Gap or Biodiversity Gap? Evaluating apparent spatial biases in community science observations of Odonata in the east-central United States
Authors: Bullion Christian M. , Bahlai Christie A. 
Published: December 2022

AbstractOdonates (dragonflies and damselflies) have become popular study organisms for insect-based climate studies, due to the taxon’s strong sensitivity to environmental conditions, and an enthusiastic following by community scientists due to their charismatic appearance and size. Where formal records of this taxon can be limited, public efforts have provided nearly 1,500,000 open-sourced odonate records through online databases, making real-time spatio-temporal monitoring more feasible. While these databases can be extensive, concerns regarding these public endeavors have arisen from a variety of sources: records may be biased by human factors (ex: density, technological access) which may cause erroneous interpretations. Indeed, records of odonates in the east-central US documented in the popular database iNaturalist bear striking patterns corresponding to political boundaries and other human activities. We conducted a ‘ground-truthing’ study to examine these patterns in an area where community science reports indicated variable abundance, richness, and diversity which appeared to be linked to observation biases. Our observations were largely consistent with patterns recorded by community scientists, suggesting these databases were indeed capturing representative biological trends and raising further questions about environmental drivers in the observed data gaps.
The Epithelial Na+Channel UNC-8 promotes an endocytic mechanism that recycles presynaptic components from old to new boutons in remodeling neurons
Authors: Cuentas-Condori Andrea , Chen Siqi , Krout Mia , Gallick Kristin , Tipps John , Flautt Leah , Richmond Janet E. , Miller David M. 
Published: December 2022

SummaryPresynaptic terminals are actively relocated during development to refine circuit function, but the underlying cell biological mechanisms are largely unknown. InC. elegans, the presynaptic boutons of GABAergic DD neurons are moved to new locations during early larval development. We show that developmentally regulated expression of a presynaptic Epithelial Na+Channel (ENaC), UNC-8, promotes a Ca2+-dependent mechanism, resembling Activity-Dependent Bulk Endocytosis (ADBE), that dismantles presynaptic material for reassembly at nascent DD synapses. ADBE normally functions in highly active neurons to accelerate local recycling of synaptic vesicles. We show that DD presynaptic remodeling depends on canonical features of ADBE including elevated intracellular Ca2+, the phosphatase Calcineurin and its targets, dynamin and the F-BAR protein syndapin, and Arp2/3-driven actin polymerization. Thus, our findings suggest that a native mechanism (ADBE) for maintaining neurotransmitter release at local synapses has been repurposed, in this case, to dismantle presynaptic terminals for reassembly at new locations.HighlightsDeveloping GABAergic neurons dismantle presynaptic terminals for reassembly at new locations.The DEG/ENaC protein, UNC-8, promotes presynaptic disassembly and recyclingCa2+-dependent endocytosis drives presynaptic disassembly and recycling to new boutons
Human voltage-gated Na+and K+channel properties underlie sustained fast AP signaling
Authors: Wilbers René , Metodieva Verjinia D. , Duverdin Sarah , Heyer Djai B. , Galakhova Anna A. , Mertens Eline J. , Versluis Tamara D. , Baayen Johannes C. , Idema Sander , Noske David P. , Verburg Niels , Willemse Ronald B. , de Witt Hamer Philip C. , Kole Maarten H. P. , de Kock Christiaan P.J. , Mansvelder Huibert D. , Goriounova Natalia A. 
Published: December 2022

AbstractHuman cortical pyramidal neurons are large, have extensive dendritic trees, and yet have surprisingly fast input-output properties: rapid subthreshold synaptic membrane potential changes are reliably encoded in timing of action potentials (APs). Here, we tested whether biophysical properties of voltage-gated sodium (Na+) and potassium (K+) currents in human pyramidal neurons can explain their fast input-output properties. Human Na+and K+currents exhibited more depolarized voltage-dependence, slower inactivation and faster recovery from inactivation compared with their mouse counterparts. Computational modeling showed that despite lower Na+channel densities in human neurons, the biophysical properties of Na+channels resulted in higher channel availability and contributed to fast AP kinetics stability. Finally, human Na+channel properties also resulted in a larger dynamic range for encoding of subthreshold membrane potential changes. Thus, biophysical adaptations of voltage-gated Na+and K+channels enable fast input-output properties of large human pyramidal neurons.One-Sentence SummaryBiophysical properties of Na+and K+ion channels enable human neurons to reliably encode fast inputs into output.
Dual-color single molecule localization microscopy on transparent polymer waveguide chips
Authors: Engdahl Anders Kokkvoll , Dutta Surjendu Bikash , Belle Stefan , Schürstedt Jasmin , Szafranska Karolina , McCourt Peter , Hellmann Ralf , Huser Thomas , Schüttpelz Mark 
Published: December 2022

AbstractPhotonic waveguide chips offer near-field excitation of biological samples, which enables cost-effective, large field-of-view super-resolution microscopy without the need for high numerical aperture (NA) objective lenses. Single molecule localization based super-resolution microscopy that requires high illumination intensities is currently limited to solid state photonic waveguide chips composed of hard-coated, high NA planar waveguides deposited on opaque substrates. These platforms do not permit epi-detection of fluorescence through the substrate, which limits the use of photonic waveguide chips to the upright configuration. Additionally, the detection efficiency is reduced because the majority of the fluorescence emission is directed towards the high refractive index substrate. A low cost waveguide chip based on a polymer core material deposited on common #1.5 coverslips that is easy to produce was recently demonstrated. Here, a platform that is capable of performing single-molecule localization microscopy (SMLM) of biological samples using polymer-based photonic waveguide chips is presented, enabling super-solution microscopy in the inverted microscope configuration. Super-resolved imaging of two different structures of the cytoskeleton in primary liver sinusoidal endothelial cells (LSECs) by two popular SMLM methods,dSTORM and DNA-PAINT, down to 23 nm is demonstrated.
Plasmodium falciparumadapts its investment into replicationversustransmission according to the host environment
Authors: Abdi Abdirahman I. , Achcar Fiona , Sollelis Lauriane , Silva-Filho Joao Luiz , Mwikali Kioko , Muthui Michelle , Mwangi Shaban , Kimingi Hannah W. , Orindi Benedict , Kivisi Cheryl Andisi , Alkema Manon , Chandrasekar Amrita , Bull Peter C. , Bejon Philip , Modrzynska Katarzyna , Bousema Teun , Marti Matthias 
Published: December 2022

AbstractThe malaria parasite life cycle includes asexual replication in human blood, with a proportion of parasites differentiating to gametocytes required for transmission to mosquitoes. Commitment to differentiate into gametocytes, which is marked by activation of the parasite transcription factorap2-g, is known to be influenced by host factors but a comprehensive model remains uncertain. Here we analyze data from 828 children in Kilifi, Kenya with severe, uncomplicated, and asymptomatic malaria infection over 18 years of falling malaria transmission. We examine markers of host immunity and metabolism, and markers of parasite growth and transmission investment. We find that inflammatory responses and reduced plasma lysophosphatidylcholine levels are associated with markers of increased investment in parasite sexual reproduction (i.e., transmission investment) and reduced growth (i.e., asexual replication). This association becomes stronger with falling transmission and suggests that parasites can rapidly respond to the within-host environment, which in turn is subject to changing transmission.
HTRX: an R package for learning non-contiguous haplotypes associated with a phenotype
Authors: Yang Yaoling , Lawson Daniel 
Published: December 2022

AbstractSummaryHaplotype Trend Regression with eXtra flexibility (HTRX) is an R package which uses cross-validation to learn sets of interacting features for a prediction. HTRX identifies haplotypes composed of non-contiguous single nucleotide polymorphisms (SNPs) associated with a phenotype. To reduce the space and computational complexity when investigating many features, we constrain the search by growing good feature sets using ‘Cumulative HTRX’, and limit the maximum complexity of a feature set.AvailabilityHTRX is implemented in R and is available under GPL-3 license from CRAN and Github at:https://github.com/YaolingYang/HTRX.Contactyaoling.yang@bristol.ac.uk
Intermittent treatment with elamipretide preserves exercise tolerance in aged female mice
Authors: Campbell Matthew D. , Samuelson Ashton T. , Chiao Ying Ann , Sweetwyne Mariya T. , Ladiges Warren C. , Rabinovitch Peter S. , Marcinek David J. 
Published: December 2022

AbstractThe pathology of aging impacts multiple organ systems including the kidney, skeletal, and cardiac muscle. Long-term treatment with the mitochondrial targeted peptide elamipretide has previously been shown to improvein vivomitochondrial function in aged mice that is associated with increased fatigue resistance and treadmill performance, improved cardiovascular diastolic function, and glomerular architecture of the kidney. However, elamipretide is a short tetrameric peptide that is not orally bioavailable limiting its routes of administration. This study tested whether twice weekly intermittent injections of elamipretide could recapitulate the same functional improvements as continuous long-term infusion. We found that intermittent treatment with elamipretide for 8 months preserved endurance running in mice, skeletal muscle force production, and left ventricular mass but did not affect heart or kidney function as previously reported using continuous treatment.
Population mortality impacts of the rising cost of living in Scotland: modelling study
Authors: Richardson Elizabeth , McCartney Gerry , Taulbut Martin , Douglas Margaret , Craig Neil 
Published: December 2022

AbstractObjectivesTo estimate the potential impacts of unmitigated and mitigated cost of living increases on real household income, mortality, and mortality inequalities in Scotland.DesignModelling study.SettingScotland, 2022/23.ParticipantsA representative sample of 5,602 Scottish individuals (within 2,704 households) in the 2015/16 Family Resources Survey. We estimated changes in real household income associated with differential price inflation (based on proportion of household spending on different goods and services, by income group), both with and without mitigating UK Government policies, and scaled these to the Scottish population. We estimated mortality effects using a cross-sectional relationship between household income and mortality data, by deprivation group.InterventionsBaseline was Scotland in 2022/23 with the average wage and price inflation of preceding years. The comparison scenarios were unmitigated cost of living increases, and mitigation by the UK Government’s Energy Price Guarantee (EPG) and Cost of Living Support payments.Main outcome measuresPremature mortality rate and life expectancy at birth by Scottish Index of Multiple Deprivation (SIMD) group, and inequalities in both.ResultsUnmitigated price inflation was 14.9% for the highest income group and 22.9% for the lowest. UK Government policies partially mitigated impacts of the rising cost of living on real incomes, although households in the most deprived areas of Scotland would still be £1,400 per year worse off than at baseline. With the mitigating measures in place, premature mortality was estimated to increase by up to 6.4%, and life expectancy to decrease by up to 0.9%. Effects would be greater in more deprived areas, and inequalities would increase as a result.ConclusionsLarge and inequitable impacts on mortality in Scotland are predicted if real-terms income reductions are sustained. Progressive Cost of Living Support payments are not sufficient to offset the mortality impacts of the greater real income reductions in deprived areas.What is already known on this topicOver the last decade, life expectancy in Scotland has stalled and inequalities have increased.Income reductions have been related to increased mortality risk, hence the economy matters for public health.The impacts of the rising cost of living and mitigating policies on mortality and inequalities require estimation to inform policymaking.What this study addsThe mortality impacts of inflation and real-terms income reduction are likely to be large and negative, with marked inequalities in how these are experienced.Current public policy responses are not sufficient to protect health and prevent widening inequalities.Bolder and more progressive policy responses are required if health is to improve and health inequalities are to narrow.