bluuebunny/crossref_metadata_2025_split huggingface dataset:
A4GALT mediated the glycosylation of B7-H3 in human colorectal cancer cell lines
Authors: Li Fuchao , Han Dan , Chen Mengxi , Li Jingmei , Yang Li , Wang Weipeng , Yu Chenggong 
Published: February 2022

Abstract
B7-H3 is one of the most important members of the B7/CD28 family, and its expression level is abnormally high in a variety of tumors. B7-H3 inhibits T cell activation via binding to the corresponding receptors on T cells, thereby mediating tumor immune escape. Glycosylation is a common post-translational modification of proteins, which plays an essential role in protein expression patterns and biological functions. Current evidence has shown that the abnormal glycosylation of B7-H3 in tumors is of great significance for protein expression and ligand-receptor binding. Therefore, in-depth exploration of the underlying mechanism of glycosylation modification of B7-H3 is expected to provide new insights for tumor immunotherapy. To investigate the underlying mechanism of glycosyltransferase-mediated glycosylation of B7-H3. Firstly, the CHIPBase database was used to screen glycosyltransferases with a high correlation with the protein expression of B7-H3. Then their siRNAs were designed and synthesized to transfect into cells, and the western blotting assay was performed for further screening. Secondly, the siRNA and overexpression plasmid of the screened glycosyltransferase were respectively transfected into cells to verify the effect on the expression level of B7-H3. Thirdly, the effect of glycosyltransferase on the expression level of B7-H3 was explored by changing its substrate level. Finally, the co-immunoprecipitation experiment was conducted to verify whether protein binding existed between B7-H3 and the glycosyltransferase. The glycosyltransferase called A4GALT had the highest correlation with the protein expression of B7-H3. After knocking down or overexpressing A4GALT, the protein expression level of B7-H3 both changed significantly. When knocked down GALT to reduce the galactosyl donors, B7-H3 was significantly down-regulated. And B7-H3 was up-regulated while increasing the galactose in the medium. In addition, the Co-immunoprecipitation experiment proved that there was protein binding between B7-H3 and A4GALT. A4GALT can positively regulate the expression of B7-H3, and changing the level of galactosyl donors can also positively regulate the expression of B7-H3. There is a protein interaction between A4GALT and B7-H3.
A Note on Categories about Rough Sets
Authors: Syau Yu-Ru , Lin En-Bing , Liau Churn-Jung 
Published: February 2022

Abstract
Using the concepts of category and functor, we provide some insights and prove an intrinsic property of the category AprS of approximation spaces and relation-preserving functions, the category RCls of rough closure spaces and continuous functions, and the category RInt of rough interior spaces and continuous functions. Furthermore, we define the category IS of information systems and O-A-D homomorphisms, and establish the relationship between the category IS and the category AprS by considering a subcategory NeIS of IS whose objects are information systems and whose arrows are nonexpensive O-A-D homomorphisms with surjective attribute mappings.
Practical Consideration For Successful Sequential Tumor Biopsies In First-In-Human Trials
Authors: Koyama Takafumi , Shimizu Toshio , Sato Jun , Katsuya Yuki , Iwasa Satoru , Kondo Shunsuke , Yoshida Tatsuya , Sudo Kazuki , Nishino Makoto , Takiguchi Yuichi , Yonemori Kan , Yamamoto Noboru 
Published: February 2022

Abstract
Purpose: In first-in-human (FIH) trials, sequential tumor biopsies, i.e., two consecutive tumor biopsies, the first perfomed at baseline (pretreatment) and the second during the early treatment period (on-treatment), provide proof of concept in investigational new drugs. Methods: We evaluated the success of sequential tumor biopsies in FIH trials, and explored approaches for improved success rates. We retrospectively reviewed the sequential tumor biopsies required in 17 of 52 FIH trials conducted from 2015 to 2020. Results: One hundred and thirty-eight patients were identified. Success of either pretreatment or on-treatment biopsy alone, and of sequential tumor biopsies, was defined as the acquisition of viable tumor cells and as obtaining tumor cells from both biopsy specimens, respectively. The success rates of pretreatment and on-treatment biopsy were 98.6% and 94.2%, respectively, and of sequential tumor biopsies was 70.3%. Adverse events associated with the pretreatment biopsies (33.3% positive; 72.0% negative) and timing of the first imaging assessment (before on-treatment biopsy = 40.0%; after on-treatment biopsy = 82.7%) correlated with successful sequential tumor biopsies. The reasons for unsuccessful sequential tumor biopsies could be categorized into two groups: 1) patient refusal of the on-treatment biopsy (most frequently due to early disease progression); and 2) absence of tumor cells in the pretreatment or on-treatment biopsy specimen. Conclusion: We propose an approach to achieving greater success in sequential tumor biopsies in FIH trials; the first imaging assessment during the study should be scheduled after on-treatment biopsy.(Registration number UMIN000042487, Date of registration November 18, 2020)
Time Domain Diversity Combining with Delay-and-Advanced Operation in Two Layered Asymmetrically Clipped Optical OFDM System
Authors: Baig Mohammed Salman , Abas Ahmed Fauzi , ALRESHEEDI MOHAMMED THAMER , Mahdi Mohd Adzir 
Published: February 2022

Abstract
This paper discusses the implementation of time domain delay-and-advanced operation with diversity combining at the transmitter for a two-layered LACO-OFDM system. This technique utilizes all the subcarriers and improves the BER performance. The new improved 2-LACO-OFDM system achieves 2.7 dB, 3.3 dB and 3.7dB better optical signal to noise ratio (OSNR) than the ACO-TDDC, 2-LACO-TDDC, 3-LACO-TDDC respectively.
Hepatotoxicity Following Immune Checkpoint Inhibitor Therapy in Metastatic Lung Adenocarcinoma: A Case Report
Authors: Lou Shike , Wang Xiaolin , Yuan Fei , Zhao Gangde , Lin Lanyi , Ding Yezhou , Liu Kehui , Xie Qing , Bao Shisan , Wang Hui 
Published: February 2022

Abstract
Immune checkpoint inhibitors (ICIs) are mAbs against cytotoxic T-lymphocyte antigen 4 (CTLA-4), programmed death-1 receptor (PD-1) and programmed death-ligand 1 (PD-L1), which the inhibitory pathways that leads to T cell inactivation. Here, we report a case of ICI-induced immune-mediated hepatotoxicity with liver biopsy. A 63-year-old man with brain metastasis from lung adenocarcinoma was treated with three times of g knife treatment, following by a combination of chemotherapy [carboplatin and (550 mg) + pemetrexed (1000 mg)] plus bevacizumab (anti-VEGF monoclonal antibody) (600 mg D1) and camrelizumab (anti-PD-1 mAb) (200 mg D1). Around 21 days later, abnormal liver function was detected. He received a diagnosis of ICI-induced liver injury after liver biopsy. The liver function of this patient was recovered, following methylprednisolone treatment. Such data offers useful information for clinicians to deal with cancer patients for ICI treatment, suggesting that liver function must be closely monitored.
Association between neonatal hyperbilirubinemia and hypoglycemia in Chinese women with diabetes in pregnancy and influence factors
Authors: Chen Zhen , He Jing , Song Jiayang , Zou Zhijie , Fan Xiaoxiao , Tian Ruixue , Xu Jingqi , Yan Yu , Bai Jinbing , Liu Yanqun , Chen Xiaoli 
Published: February 2022

Abstract
This retrospective study aimed to investigate the correlation between neonatal hyperbilirubinemia (NHB) and hypoglycemia (NH) in Chinese women with diabetes in pregnancy (DIP), and the influencing factors. All the data were collected July 1, 2017 and June 30, 2020, and 10,558 Chinese women with DIP and live births were included. Two separate multivariate binary stepwise forward logistic regression analysis calculated OR with 95% CI. The prevalence rates of NHB and NH was respectively 6.47% and 6.42% among women with DIP. The comorbidity of both diseases was 1.15%. NH were 1.73 times (OR 1.73, 1.21-2.47) more likely to have hyperbilirubinemia. NHB is positively correlated with NH (OR 3.61, 2.51-5.20). Increased gestational age has a protective effect on both NH (OR 0.81, 0.77-0.87) and NHB (OR 0.85. 0.76-0.95). Abnormal placental morphology is related to NH (OR 1.47, 1.11-1.95) and NHB (OR 1.41, 1.02-1.98). Regarding neonatal outcomes, congenital heart disease (CHD) (OR 3.00, 2.10-4.29; and OR 7.67, 5.50-10.70) and neonatal anemia (OR 4.90, 2.13-11.26; and OR 6.16, 2.34-16.22) were risk factors of both. NHB and NH were significantly correlated in women with DIP. The offspring of DIP with multiple risk factors have a significantly increased risk of neonatal hyperbilirubinemia.
Designing an Optimization Model for Green Closed-Loop Supply Chain Network of Heavy Tire by Considering Economic Pricing under Uncertainty
Authors: Amirian Javad , amoozadkhalili hossein , Mehrabian Ahmad 
Published: February 2022

Abstract
The conditions of global competition and environmental sensitivities have made organizations and factories to collect returned products. In such a way that these organizations have tried rehabilitate, recycle or destroy these products in order to protect the environment. This paper propose a mathematical model for the green closed-loop supply chain network of heavy tire by considering the economic pricing of its products under conditions of uncertainty, which economically determines the price and leads to more profitability. In addition, the relevant model is a two-objective fuzzy model, the first objective of which is to minimize costs and maximize profits and the second objective is to minimize environmental issues. The proposed model can also determine the optimal location of each center based on potential locations, the optimal amount of production, distribution, collection, recycling, as well as the reproduction of products. The ε-constraint method is used to solve the model with two objective functions; this method ensures strong Pareto optimal answers and prevents weak Pareto answers. Independent two-sample t-test is used to verify the results of certain and uncertain models in the studied model. In order to evaluate the effectiveness and profitability of the proposed method, a case study in the field of heavy tires is finally used, through which very useful results are obtained.
Revised Leachate Pollution Index (r-LPI): A Tool to Quantify Pollution Potential of Landfill Leachate
Authors: Bisht Tribhuwan Singh , Kumar Dinesh , Alappat Babu J. 
Published: February 2022

Abstract
The leachate pollution index (LPI), a technique to quantify the contamination potential of landfill leachate, was developed in 2003. Since then, numerous factors have challenged the relevance of LPI, including advancements in technology, the long-term reliability of these indicators, the incidence of emerging contaminants, and the LPI’s efficacy. As a result, using LPI as a benchmark can lead to misinterpretation of the magnitude of leachate Pollution. To mitigate this, a revised leachate pollution index (r-LPI) was developed, which is more precise and robust in assessing the Pollution potential of landfill leachate. This article presents a comprehensive account of the development of r-LPI. The r-LPI was developed by incorporating fuzzy technique with a multi-criteria decision-making technique (MCDM), wherein the inputs from 60 experts in the field of the environment, specifically solid waste management, were acquired at different stages during its development. The fuzzy Delphi method (FDM) was used to select the parameters. The fuzzy analytic hierarchy process (FAHP) was used to compute the relative weights of the parameters and sub-index curves were used for normalization of the parameters. As an application, the LPI and the r-LPI of the Bhalswa, Okhla, and Ghazipur landfills were calculated. The results indicate that r-LPI provides a more comprehensive prediction of leachate Pollution than the LPI.
Intact fibroblast growth factor 23 tested by a fully automated assay: clinical correlates and prognostic value in chronic systolic heart failure
Authors: Vergaro Giuseppe , Franco Annamaria Del , Gentile Francesco , Castiglione Vincenzo , Aimo Alberto , Saponaro Federica , Masotti Silvia , Prontera Concetta , Fusari Niccolò , Emdin Michele , Passino Claudio 
Published: February 2022

Abstract
Background Fibroblast growth factor-23 (FGF23) has been associated to left ventricular (LV) hypertrophy and heart failure (HF) severity. We aimed to investigate clinical correlates and prognostic value of intact FGF23 (iFGF23) in HF patients.Methods Patients with systolic HF (left ventricular ejection fraction - LVEF<50%) were prospectively enrolled. iFGF23 was tested with a fully automated immuno-chemiluminescent assay. Patients were divided into iFGF23 tertiles and followed-up for all-cause death, cardiac death, and the composite of all-cause death and HF hospitalizations. Results We enrolled 150 patients (82% males; median age 65 years). First, second, and third iFGF23 tertiles were <35.2 pg/mL, 35.2-50.9 pg/mL, and >50.9 pg/mL. Patients in the upper iFGF23 tertile had lower LVEF (p=0.014), higher NT-proBNP (p=0.001), and lower peak VO2 (p<0.001). Phosphate, estimated glomerular filtration rate (eGFR), and LV mass index were independently associated to iFGF23 (all p<0.05). Patients in the upper tertile were at higher risk of all-cause death (p=0.001), cardiac death (p=0.001), and of the composite of all-cause death and HF hospitalizations (p=0.003). iFGF-23 was an independent predictor of all the end-points after adjustment for age, LVEF, eGFR, and NT-proBNP. Conclusions Circulating iFGF23 is associated with renal dysfunction, disease severity and outcome in systolic HF.
The effect of chronic stress and its preconditioning on spatial memory as well as hippocampal LRP1 and RAGE expression in a streptozotocin-induced rat model of Alzheimer’s disease
Authors: Taghadosi Zohreh , Zarifkar Asadollah , Razban Vahid , aligholi Hadi 
Published: February 2022

Abstract
According to available evidence, prolonged or chronic exposure to stress is detrimental to various brain structures, including the hippocampus. The current study examined the expression of two critical blood-brain barrier receptors required for amyloid-beta clearance to understand better the mechanism by which chronic stress impairs learning and memory in patients with Alzheimer's disease (AD). Rats were bilaterally injected with streptozotocin (STZ) (3mg/kg) using the intracerebroventricular (ICV) technique to induce the Alzheimer's model. Additionally, they were subjected to foot shock (1mA, 1Hz) for 10s every 60s (1h/day) for ten consecutive days prior to and following STZ injection. The Morris Water Maze (MWM) test was used to assess spatial learning and memory. Real-time PCR was used to determine Low-density lipoprotein receptor-related protein-1 (LRP1) and receptor for advanced glycation end-products (RAGE) mRNA levels in the hippocampus. Moreover, the animals' body weights were determined as physiological parameters in all groups. The results indicated that 10-day chronic electric foot shock stress reduced body weight, impaired spatial learning and memory, decreased hippocampal LRP1 mRNA expression, and increased hippocampal RAGE mRNA expression in a rat AD model. It can be concluded that chronic stress in conjunction with AD alters the expression of LRP1 and RAGE in the hippocampus. The findings pave the way for scientists to develop novel treatment strategies for AD.
Characterization of Hearing-Impairment in Generalized Arterial Calcification of Infancy (GACI)
Authors: Theng Elizabeth Hartanti , Brewer Carmen C , Oheim Ralf , Zalewski Christopher K , King Kelly A , Delsmann Maximillian M , Rolvien Tim , Gafni Rachel I , Braddock Demetrios T , Kim H Jeffrey , Ferreira Carlos R 
Published: February 2022

Abstract
Background and ImportanceHearing loss (HL) has been sporadically described, but not well characterized, in Generalized Arterial Calcification of Infancy (GACI), a rare disease in which pathological calcification typically presents in infancy. ObjectivesThis study aims to describe the clinical audiologic and otologic features and potential etiology of hearing impairment in GACI and gain pathophysiological insight from a murine model of GACI.DesignCross-sectional cohort study of individuals with GACI. Murine ossicle micromorphology of the ENPP1asj/asj mutant compared to wild-type.SettingClinical research hospital; basic science laboratoryParticipantsNineteen individuals with GACI who met clinical, biochemical, and genetic criteria for diagnosis.Main Outcomes and MeasuresClinical, biochemical, and radiologic features associated with hearing status. ResultsPure tone thresholds could be established in 15 (n=30 ears) of the 19 patients who underwent audiological assessments. The prevalence of HL was 50% (15/30) of ears, with conductive HL in 80% and sensorineural HL in 20%. Degree of HL was mild to moderate for 87% of the 15 ears with hearing loss. Of those patients with sufficient pure tone and middle ear function data, 80% (8/10) had audiometric configurations suggestive of ossicular chain dysfunction (OCD). Recurrent episodes of otitis media (ROM) requiring pressure-equalizing tube placement were common. In patients who underwent cranial CT, 54.5% (6/11) had auricular calcification. Quantitative backscattered electron imaging (qBEI) of murine ossicles support an OCD component of auditory dysfunction in GACI, suggesting loss of ossicular osteocytes without initiation of bone remodeling. Conclusions and RelevanceHearing loss is common in GACI; it is most often conductive, and mild to moderate in severity. The etiology of HL is likely multifactorial, involving dysfunction of the ossicular chain and/or recurrent otitis media. Clinically, this study highlights the importance of early audiologic and otologic evaluation in persons with GACI. Novel findings of high rates of OCD and ROM may inform management, and in cases of unclear HL etiology, dedicated temporal bone imaging should be considered.
Spatiotemporal differentiation and the obstacle factors influencing the coupling coordination between economic development and water pollution control capability in the Yangtze River Economic Belt
Authors: Lv Lelin , Chen Canjun , Wang Zhuofu 
Published: February 2022

Abstract
The Yangtze River Economic Belt (YREB) is a highly influential economic belt and an innovative demonstration belt for the protection and restoration of ecological and environmental systems. The dynamic coordination between economic development system (EDs) and water pollution control capability system (WPCCs) is a critical issue to be solved for regional sustainable development. However, this topic has not been adequately addressed in previous studies. To bridge this gap, this paper analyzed the spatiotemporal differentiation and obstacle factors influencing the coupling coordination between EDs and WPCCs in the Chinese Five-Year Plan based on coupling coordination degree model and obstacle degree model. The main results suggest that: (1) the comprehensive level of the comprehensive level of water pollution control capability presents an upward trend with the increase of economic development. A J-shaped relationship existed between the EDs and WPCCs. (2) the regions with rapid economic growth are mainly distributed in the Shanghai, Zhejiang, Jiangsu, Chongqing, and Sichuan. Moreover, water pollution control capability system shows this pattern, eastern regions ＞ western regions＞ central regions. (3) the coupling coordination level of each region in the YREB has improved from a moderately unbalanced development level to a superiorly balanced development level from 2006 to 2019. (4) per capita gross domestic product, gross product of tertiary industry, total volume of waste water treated, and per daily volume treated of sewage treatment facilities are the major indexes influencing the coordinated development of the EDs and WPCCs. These finding are conductive to formulating reasonable strategies for water environment protection and sustainable development and providing a direction for urban planning.
Predictors of the Development of Nab-Paclitaxel-Induced Peripheral Neuropathy in Breast Cancer Patients: Post Hoc Analysis of a Prospective, Phase II, Self-Controlled Clinical Trial
Authors: Kanbayashi Yuko , Sakaguchi Koichi , Ishikawa Takeshi , Tabuchi Yusuke , Takagi Ryo , Yokota Isao , Katoh Norito , Takayama Koichi , Taguchi Tetsuya 
Published: February 2022

Abstract
In a previous study, we showed that cryotherapy and compression therapy have comparable efficacy in preventing nab-paclitaxel-induced peripheral neuropathy. However, even with cryotherapy or compression therapy, there were patients with National Cancer Institute Common Terminology Criteria for Adverse Events (CTCAE) version 4.0 grade ≥ 2 and/or Patient Neurotoxicity Questionnaire (PNQ) grade ≥ D peripheral neuropathies. Therefore, this post hoc analysis was performed to identify predictors of nab-paclitaxel-induced peripheral neuropathy. The clinical data in this post hoc analysis were the data of 38 breast cancer patients receiving chemotherapy with nanoparticle albumin-bound paclitaxel (nab-PTX) at our outpatient chemotherapy center from August 2017 to March 2019. The number of patients was analyzed assuming that there were data for 76 hands. Variables related to the development of nab-PTX-induced peripheral neuropathy were used for regression analysis. Multivariate ordered logistic regression analysis was performed to identify predictors for the development of nab-PTX-induced peripheral neuropathy. Significant factors included smoking history [odds ratio (OR) = 4.64, 95% confidence interval (CI) = 1.60-13.5; P = 0.0048] with neuropathy evaluated by CTCAE, body mass index (BMI) (OR = 1.13, 95% CI = 1.01-1.26; P = 0.039) with neuropathy evaluated by PNQ (sensory), and smoking history (OR = 3.80, 95% CI = 1.40-10.30; P = 0.0087) and age (OR = 1.06, 95% CI = 1.01-1.11; P = 0.012) with neuropathy evaluated by PNQ (motor). In conclusion, smoking history, BMI and age were identified as significant predictors of the development of nab-PTX-induced-peripheral neuropathy.
Selection of Oxygen Reduction Catalysts For Secondary Tri-Electrode Zinc-Air Batteries
Authors: Loh Adeline , Trudgeon David P. , Li Xiaohong , Liu Mao-Cheng , Kong Ling-Bin , Walsh Frank C. 
Published: February 2022

Abstract
The selection of a highly efficient, low-cost, yet durable ORR electrocatalyst is important for secondary Zn-air cell applications. ORR activities of single and mixed metal oxide and carbon electrocatalysts were studied using rotating disc electrode (RDE) measurements, Tafel slope and Koutecky-Levich plots. It was found that MnOx combined with XC-72R demonstrated high ORR activity and good stability – up to 100 mA cm-2. The performance of the selected ORR electrode was thereafter tested in a custom-built secondary Zn-air cell in a tri-electrode configuration. The effects of current density, electrolyte molarity, temperature, and oxygen purity on the performance of the OER and ORR electrode were investigated. Finally the durability of the secondary Zn-air system was assessed, demonstrating energy efficiencies of 58-61% at 20 mA cm-2 over 40 h in 4 M NaOH + 0.3 M ZnO at 333 K.