bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein signature of human white blood cells enables biochemical tracking of rare neurological diseases
Authors: Hentschel Andreas , Rademacher Nina , Schara-Schmidt Ulrike , Meyer Nancy , Sickmann Albert , Güttsches Anne-Katrin , Vorgerd Matthias , Schorling David , Roos Andreas 
Published: May 2022

Abstract
Background: The identification of pathomechanisms leading or contributing to the clinical manifestation of rare neurological diseases such as neuromuscular diseases (NMD) is crucial. The study of the molecular basis of these diseases is also important for the definition of starting points for (new) therapeutic intervention concepts as well as for testing of pathogenicity of genetic variants identified by sequencing approaches. However, these studies are frequently limited by the availability of human biomaterial. Methods: Human white blood cells were used to generate a spectral library using pH8-fractionation followed by nano liquid chromatography coupled to tandem mass spectrometry. To demonstrate the suitability of this approach, we next subjected proteins isolated from white blood cells derived from two CHKB patients to a data independent acquisition approach. Paradigmatic proteomic findings were confirmed by immunofluorescence in a muscle biopsy derived from one of these patients.Results: To investigate if human white blood cells might serve as valuable biomaterial for (molecular) studies of NMD, we generated a protein library cataloguing 7543 proteins covering 52.2 % of proteins for which mutations in corresponding genes are known to be causative for NMDs. Based on these findings, we aimed to further demonstrate the suitability of this in vitro model to study the etiopathology of a rare NMD caused by bi-allelic variants within CHKB. Dysregulation of paradigmatic proteins could be confirmed in the quadriceps muscle biopsy of one of these patients and protein-functions provided new insights into the underlying pathophysiology indicating affection of subcellular structures (functionally) connected to mitochondria such as the Endoplasmic Reticulum (ER) and cytoskeleton. Moreover, altered protein clearance is indicated by our combined proteomic and immunofluorescence findings.Conclusions: Our combined data reveal that human white blood cells may serve as an in vitro system to study the molecular etiology of rare neurological diseases exemplified on a rare mitochondrial NMD based on pathogenic variants affecting CHKB in an unbiased fashion.
Association Between Muscle Mass Quantity and Quality and Muscle Strength in Adults with Obesity
Authors: Streb¹ Anne Ribeiro , Benedet¹ Jucemar , Rosa¹ Fernanda , Barcelos¹ Guilherme Tadeu , Gerage¹ Aline Mendes , Duca¹ Giovani Firpo Del 
Published: May 2022

Abstract
Background: This cross-sectional study aimed to verify the association between muscle quantity and quality and muscle strength in obese adults.Methods: Lean mass (LM) and intracellular water (ICW) were obtained using bioelectrical impedance. Muscle quantity was expressed in kg for LM, while muscle quality was determined by ICW and the ratio of ICW/LM. Maximum strength was measured by the one-repetition maximum test (1RM) in bench press and leg press exercises at 45º. The score for total strength (Z-score) was given by the average of scores for each test. The statistical analysis included Spearman's correlation, whose results were expressed as correlation coefficients (r); crude and adjusted linear regression, expressed as adjusted coefficients of determination (R2); Bayesian Information Criterion (BIC); and Akaike Information Criteria (AIC). Results: Sixty-nine adults of both sexes with a mean age of 34 (± 7.1) years participated in the study. A strong positive correlation was found between the amount of LM and muscle strength (r = 0.83; p < 0.001). There was also a positive correlation between ICW and the ratio of ICW/LM, indicators of muscle quality, and muscle strength, resulting in strong (r = 0.83 p < 0.001) and moderate (r = 0.40 p < 0.001) magnitudes, respectively. Additionally, ICW had better predictive power for muscle strength (R2 adjusted = 0.98; AIC: 161.8483; BIC: 175.253).Conclusion: Muscle quantity and quality presented good predictive capacity for muscle strength. Assessing muscle quality can provide further clarification on how the muscle is able to produce strength in adults with obesity.
The effect of serum estrogen levels in early pregnancy on the rate of hypertensive disorders of pregnancy after frozen embryo transfer: a case control study
Authors: Hsieh Yun-Chiao , Kao Tzu-Ching , Yang Ih-Jane , Yang Po-Kai , Chao Kuang-Han , Chen Mei-Jou , Yang Jehn-Hsiahn , Chen Shee-Uan 
Published: May 2022

Abstract
BackgroundEstrogen (E2) is a key regulator of trophoblast invasion and remodeling of uterine decidual spiral arterioles during placentation. However, there is little research on the effects of E2 in early pregnancy on placentation and subsequent preeclampsia. This study aims to evaluate the relationship between the rate of hypertensive disorders of pregnancy (HDP) and serum estrogen levels in early pregnancy following frozen embryo transfer (FET).MethodsThis retrospective study included all patients who conceived after FET with singleton delivery in the National Taiwan University Hospital from January 1, 2006, to December 31, 2020. A total of 729 patients were included in the analysis. The primary outcome was the rate of HDP.ResultsSerum estrogen levels at the fourth and fifth gestational weeks were significantly different between women with and without HDP after FET (393.4 ± 235.7 vs 479.0 ± 261.2 pg/ml, p=0.007 and 492.0 ± 274.5 vs 563.9 ± 263.0 pg/ml, p=0.015, respectively). Multivariable logistic regression with generalized estimating equations showed that each 100-pg/mL increase in estrogen levels increased the risk of HDP by 12% (p = 0.006) after adjustment for confounders. In a subanalysis stratified using FET protocol type (natural cycle, n = 225; hormone replacement cycle, n = 504), an increased rate of HDP (P-trend = 0.021 and 0.014, respectively) was observed with increasing estrogen level quartile at the fourth and fifth gestational weeks in the hormone replacement cycles, but not in the natural cycles.ConclusionsHigher estrogen levels in early pregnancy were associated with a higher rate of HDP. For better obstetric outcomes, we may alter the estradiol supplementation in FET hormone replacement cycles or choose FET natural cycles. Our results indicate a link between estrogen levels in early pregnancy and the rate of HDP. Further studies are needed to investigate the underlying mechanism.Trial registrationRetrospectively registered
Development of environmental contamination in a new hospital setting
Authors: Arkel Andreas L.E. van , Willemsen Ina , Weterings Esther , Kievits Heidi , Kluytmans Jan 
Published: May 2022

Abstract
Background
ATP measurement is frequently used to determine surface contamination in hospitals. Development of surface contamination in a new hospital building after entry into service is unknown. We aimed to evaluate the development of surface contamination in a new hospital setting.
Methods
Repeated ATP measurements were performed on 10 pre-defined fomites in eight patient rooms on two separate wards. Six rounds of ATP measurements with increasing time intervals were performed; another three rounds were performed directly after cleaning. Development of surface contamination over time was assessed. The lowest achievable RLU values were assessed by performing ATP measurements directly after cleaning.
Results
In total 580 ATP measurements were conducted. The first six rounds consisted of 480 ATP measurements. The three rounds after cleaning consisted of 100 ATP measurements. The median RLU value per round in chronological order was: 100, 396, 670, 273, 733, 351. The median RLU value per round after cleaning in chronological order was 226, 412, 226. Significant differences in median RLU values before and after opening of the new hospital were found and median RLU values directly after cleaning were significantly lower than in routine use.
Conclusions
Before commissioning of the new hospital, low surface contamination is found based on RLU values. An equilibrium in surface contamination is quickly achieved with substantial fluctuations in cleanliness between rounds of measurements.
First data on the ectoparasites (fleas, ticks, and lice) of the stone marten, Martes foina (Erxleben) in Turkey
Authors: KESKİN Adem , DİK Bilal 
Published: May 2022

Abstract
Turkey is a natural land bridge connecting Europe and Asia, and has a rich terrestrial mammalian fauna, with more than 150 species within the seven orders. However, there is limited information on ectoparasites of wild animals, especially carnivores, in Turkey. In the present study, we provide the first data of ectoparasites (fleas, ticks, and lice) of stone marten, Martes foina (Erxleben) (Mammalia: Carnivora) in Turkey. A total of 38 ectoparasite specimens, three flea species [Monopsyllus sciurorum sciurorum (Schrank) (1 male), Pulex irritans L. (1 female), Chaetopsylla rothschildi Kohaut (3 males, 8 females)], two tick species [Haemaphysalis erinacei Pavesi (18 males, 4 females), Ixodes redikorzevi Olenev (1 female)], and a louse species [Stachiella retusa (Burmeister) (1 male and 1 nymph)], were collected from two road-killed stone marten in two different localities in Tokat province of Turkey. Martes foina is a new host record for all ectoparasite species detected in this study for Turkey. In addition, the presence of Stachiella retusa was reported in Turkey for the first time.
A multicenter study on surgical procedure selection and risk factor analysis of postoperative complications after TIP and Duckett hypospadias repair
Authors: Fang YiWei , Sun Ning , Song HongCheng , Zhang WeiPing , Tang YunMan , Huang LuGang , Yang Yi , Chao Min , Ma Hong , Zhang JingTi , Zhang XuHui , Li ShouLin , Li Ning , Chen Chao , He DaWei , Wu WenBo , Xie Hua , Guan Yong 
Published: May 2022

Abstract
Background: Hypospadias is a common congenital malformation in pediatric urology with surgery being the only curative treatment. Although there are hundreds of surgical methods for hypospadias, no single method can treat all types, and there are still high rates of postoperative complications. We performed this study to investigate surgical procedure selection and perform risk factor analysis of postoperative complications in hypospadias repair.Methods: Retrospective analysis was performed of complete clinical and follow-up data of children with hypospadias who were treated and followed up at 15 children's clinical centers in Mainland China from December 2018 to December 2019. Children were divided into groups according to Barcat classification and surgical methods in order to analyze the surgical choice for different types of hypospadias and the influencing factors of different surgical methods for complications.Results: In total, 1011 patients were followed up for 26 months. According to Barcat classification, there were 248 cases of distal type hypospadias, 215 of intermediate, and 549 of proximal type. Duckett and tubularized incised plate (TIP) urethroplasty were performed in 375 (37.1%) and 336 cases (33.2%), respectively. The postoperative complication rate of distal hypospadias was 23.4% (15.8%-57.1%), mid shaft 29.0% (22.7%-40.0%), and proximal 43.7% (30.2%-52.9%). Among the 375 patients in Duckett group, 192 had complications. Multivariate logistic analysis showed that the length of skin island flap (OR=3.506, 95% CI:2.258-5.442) was an independent risk factor for complications after Duckett operation (P<0.001). In TIP group, there were 336 cases with 84 complications. Multivariate logistic analysis showed that the width of urethral plate after longitudinal resection (OR=0.836, 95% CI:0.742-0.942) and glans width (OR=0.851, 95% CI: 0.749-0.965) were independent risk factors for postoperative complications after TIP (P=0.003, P=0.012).Conclusion: The width of the urethral plate and glans width were risk factors for postoperative complications after TIP. The length of skin island flap was a risk factor for complications after Duckett operation. For proximal hypospadias, when the flatness and elasticity of the urethral plate are poor or when the penis is severely curved, TIP operation should be used with caution, and transverse urethral plate operation or staged operation should be selected.
Effect of diabetic retinopathy on corneal endothelial parameters: An analytical cross- sectional study
Authors: Mortazavi Seyed-Ali-Akbar , Akhlaghi Mohammadreza , Dehghani Alireza , Pourazizi Mohsen , Malekahmadi Mohammad , Fazel Mohammadreza , Tavakoli Mehran 
Published: May 2022

Abstract
Background: To investigate the possible effect of different types of diabetic retinopathy (DR) on corneal endothelium cells in type 2 diabetes mellitus patients.Methods: In this descriptive-analytical cross-sectional study, corneal endothelium parameters including endothelial cell density (ECD), average cell size (AVG), coefficient of variation in cell size (CV), and hexagonality (Hex) were evaluated by non-contact specular microscopy.Results: One hundred and thirty-four eyes of 134 diabetic patients including 77 females (57.5%) with a mean age of 61.03 ± 8.08 years were enrolled. The overall corneal parameters in diabetic patients with and without retinopathy were not significantly different (P>0.05). There is a significant relationship between CV and the duration of the disease with age variable control (B= 0.369, p-value <0.001)Conclusions: In type-2 DM patients without and with DR, corneal endothelial parameters were not a statistically significant difference. There is a significant relationship between CV and the duration of the disease with age variable control.
Fitness Dependent Optimizer for IoT Healthcare using Adapted Parameters: A Case Study Implementation
Authors: Aladdin Aso M. , Abdullah Jaza M. , Salih Kazhan Othman Mohammed , Rashid Tarik A. , Sagban Rafid , Alsaddon Abeer , Bacanin Nebojsa , Chhabra Amit , Vimal S. , Banerjee Indradip 
Published: May 2022

Abstract
This discusses a case study on Fitness Dependent Optimizer or so-called FDO and adapting its parameters to the Internet of Things (IoT) healthcare. The reproductive way is sparked by the bee swarm and the collaborative decision-making of FDO. As opposed to the honey bee or artificial bee colony algorithms, this algorithm has no connection to them. In FDO, the search agent's position is updated using speed or velocity, but it's done differently. It creates weights based on the fitness function value of the problem, which assists lead the agents through the exploration and exploitation processes. Other algorithms are evaluated and compared to FDO as Genetic Algorithm (GA) and Particle Swarm Optimization (PSO) in the original work. The key current algorithms—The Salp-Swarm Algorithms (SSA), Dragonfly Algorithm (DA), and Whale Optimization Algorithm (WOA) have been evaluated against FDO in terms of their results. Using these FDO experimental findings, we may conclude that FDO outperforms the other techniques stated. There are two primary goals for this chapter: first, the implementation of FDO will be shown step-by-step so that readers can better comprehend the algorithm method and apply FDO to solve real-world applications quickly. The second issue deals with how to tweak the FDO settings to make the meta-heuristic evolutionary algorithm better in the IoT health service system at evaluating big quantities of information. Ultimately, The target of this chapter's enhancement is to adapt the IoT healthcare framework based on FDO to spawn effective IoT healthcare applications for reasoning out real-world optimization, aggregation, prediction, segmentation, and other technological problems.
In-Vitro results of EG-Covimab-H (Lab cultivated Human polyclonal antibodies) for neutralizing SARSCoV-2 Virus &amp; safety on tissues.
Authors: Mohammed Alaa Abdelkarim , C Prof. Datuk Sri Mike 
Published: May 2022

Abstract
The debate between the active immunisation versus the passive immunisation has been the subject of discussion since the start of SARS-CoV-2 pandemic. The second debate was monoclonal antibodies versus the polyclonal antibodies in the passive vaccination, which one is more potent and safer for the patients. Which one is more valuable for the industry and the patient in the same time. This research adopted the polyclonal antibodies with new technique in production to cope with the industry needs and at the same time to achieve the best results for the patients. EG-Covimab-H, is a polyclonal antibodies derived from cultivating Switched memory B-Lymphocytes from convalescent patient (2-6 weeks after testing negative and recovering from moderate to severe infection). The isolated B Lymphocytes are cultivated in special media and antibodies were harvested and used in this research. The research focus on testing the tissue toxicity and ability of EG-Covimab-H in neutralizing the SARS-CoV-2 Virus that infected the tissue culture. Tissue culture was done on VERO-6 Cells. EG-Covimab-H was tested for 120 hours on different dilutions (1/2, 1/4, 1/8, 1/16, 1/32, 1/64, up to 1/2056). The result of the toxicity test showed 90-100% safety in the dilution of 1/8 and 1/16 respectively. Therefore, the efficacy test was done with the safe dilution and in the same dilution algorithm. The result came as effectiveness is 95%- 96% success in neutralizing the virus within 96 hours. The neutralization curve did not show any surge in the number of virus copies after applying the polyclonal antibodies and the decline in the number started to show within 48 hours.
Longitudinal predictors of informant-rated involvement of people with dementia in everyday decision-making
Authors: Sabatini Serena , Martyr Anthony , Gamble Laura D , Collins Rachel , Matthews Fiona E. , Morris Robin G. , Rusted Jennifer M. , Pentecost Claire , Quinn Catherine , Clare Linda 
Published: May 2022

Abstract
BackgroundAims We 1) described levels of informant-rated involvement of people with dementia in everyday decision-making; 2) explored whether functional, behavioral, and psychological factors related to the person with dementia and the caregiver explain variability in involvement of people with dementia in everyday decision-making at baseline; 3) estimated change in involvement in everyday decision-making over two years.Methods We used IDEAL cohort baseline, 12- and 24-months follow-up data for 1182 people with dementia and their caregivers. Measures were informant-rated everyday decision-making, informant-rated functional ability, informant-rated neuropsychiatric symptoms, cognitive capacity of the person with dementia, self-rated caregiver stress, and self-ratings of relationship quality by both person with dementia and caregiver. Linear regressions and latent growth curve models were used.Results Baseline mean score on the decision-making involvement scale was 31/45; it minimally declined over time. People with dementia who were female, single, and/or whose caregiver was younger had greater involvement in everyday decision-making than those without these characteristics. Better cognitive capacity, fewer functional difficulties, fewer neuropsychiatric symptoms, less caregiver stress, and better informant-rated relationship quality were associated with higher involvement in everyday decision-making. Self-rating of relationship quality by people with dementia was not associated with everyday decision-making.Conclusions As cognitive and functional limitations and neuropsychiatric symptoms increase, people with dementia are gradually less involved in everyday decisions. Cognitive and functional rehabilitation, together with educational resources, tailored towards dementia stage, that inform caregivers about strategies that facilitate involvement of the person with dementia in everyday decision-making, could prolong involvement in everyday decision-making.
An Updated Account on Formulations and Strategies for the Treatment of Burn Infection – A Review
Authors: Kumar Mohit , Mahmood Syed , Mandal Uttam Kumar 
Published: May 2022

Background:Burn injury is considered one of the critical injuries of the skin. According to WHO (World Health Organization), approximately 3,00,000 deaths are caused each year mainly due to fire burns, with additional deaths attributed to heat and other causes of burn e.g., electric devices, chemical materials, radioactive rays, etc. More than 95% of burn injuries occur in developing countries.Introduction:Burn injuries have been a prominent topic of discussion in this present era of advancements. Burns is one of the common and devastating forms of trauma. Burn injuries are involved in causing severe damage to skin tissues and various other body parts triggered particularly by fire, blaze, or exposure to chemicals and heated substances. They leave a long-lasting negative impact on the patients in terms of their physical and mental health.Method:The various methods and bioactive hydrogels, a viable and widely utilised approach for treating chronic wounds, remain a bottleneck. Many traditional approaches such as woven material, conventional antimicrobial agents, hydrogel sheets, and creams are utilised in wound healing. Nowadays, lipid-based nanoparticles, nanofibres systems, and foam-based formulations heal the wound.Result:The prepared formulation showed wound healing activity when tested on rat model. The nanofibres containing SSD help in the burn-wound healing study on Male Sprague Dawley (SD) rats. The healing effect on rats was examined by western blot analysis, digital camera observation, and histological analyses.Conclusion:Burn is also considered the most grievous form of trauma. Nowadays, several large and foambased formulations are used in wound healing, which heals the wound better than previously existing formulations and is less prone to secondary infection. Recently, nanofiber delivery systems have piqued the interest of academics as well as researchers because of its unique advantages and features, which include an extraordinarily high surface to volume ratio, a highly porous structure, and tiny pore size.
Immunopathogenesis and Immunogenetic Variants in COVID-19
Authors: Sagulkoo Pakorn , Plaimas Kitiporn , Suratanee Apichat , Colado Simão Andrea Name , Vissoci Reiche Edna Maria , Maes Michael 
Published: May 2022

Abstract:
Coronavirus disease 2019 (COVID-19) continues to spread globally despite the discovery of vaccines.
Many people die due to COVID-19 as a result of catastrophic consequences, such as acute respiratory
distress syndrome, pulmonary embolism, and disseminated intravascular coagulation caused by a cytokine
storm. Immunopathology and immunogenetic research may assist in diagnosing, predicting, and treating severe
COVID-19 and the cytokine storm associated with COVID-19. This paper reviews the immunopathogenesis
and immunogenetic variants that play a role in COVID-19. Although various immune-related genetic variants
have been investigated in relation to severe COVID-19, the NOD-like receptor protein 3 (NLRP3) and interleukin
18 (IL-18) have not been assessed for their potential significance in the clinical outcome. Here, we a) summarize
the current understanding of the immunogenetic etiology and pathophysiology of COVID-19 and the
associated cytokine storm; and b) construct and analyze protein-protein interaction (PPI) networks (using enrichment
and annotation analysis) based on the NLRP3 and IL18 variants and all genes, which were established
in severe COVID-19. Our PPI network and enrichment analyses predict a) useful drug targets to prevent the onset
of severe COVID-19, including key antiviral pathways such as Toll-Like-Receptor cascades, NOD-like receptor
signaling, RIG-induction of interferon (IFN) α/β, and interleukin (IL)-1, IL-6, IL-12, IL-18, and tumor
necrosis factor signaling; and b) SARS-CoV-2 innate immune evasion and the participation of MYD88 and
MAVS in the pathophysiology of severe COVID-19. The PPI network genetic variants may be used to predict
more severe COVID-19 outcomes, thereby opening the door for targeted preventive treatments.