bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation of chitosan/β-Cyclodextrin composite membrane and its adsorption mechanism for protein
Authors: Zhao Jun , Dou Xin , Tang Qilong , Qiu Peng , Li Junbo , Gou Dongxia , Liu Tong 
Published: June 2022

Abstract
Food wastewater contains a large amount of protein, which will cause water pollution. In order to solve the problem of poor adsorption protein performance and easy disintegration by a pure chitosan membrane, the chitosan/modified β-cyclodextrin (CS/β-CDP) composite membranes were prepared for the adsorption of bovine serum albumin (BSA) in this work. The effect of preparation conditions (the mass ratio of CS and β-CDP, preparation temperature and glutaraldehyde addition) and adsorption conditions (temperature and pH) on BSA the prepared CS/β-CDP composite membrane was investigated in detail. The physical and chemical properties of pure CS membrane and CS/β-CDP composite membrane was investigated. The results showed that CS/β-CDP composite membrane has higher tensile strength, elongation at break, Young's modulus, contact angle properties, and lower swelling degree. The physicochemical and morphological attributes of composite membrane before and after adsorption of BSA were characterised by SEM, FT-IR and XRD. The results showed that the CS/β-CDP composite membrane adsorbed BSA by both physical and chemical mechanisms, and the adsorption isotherm, kinetics and thermodynamic experiments further confirmed its adsorption mechanism. As a result, the CS/β-CDP composite membrane of absorbing BSA was successfully fabricated, demonstrating the potential application prospect in environmental protection.
Considering soil profile improved barley-pea mixed cropping evaluation in hilly landscape - central Italy
Authors: Tavoletti Stefano , Cocco Stefania , Corti Giuseppe 
Published: June 2022

Abstract
Purpose
Hilly soils of central Italy are characterized by high variability of physicochemical properties that influence standard agronomic practices. Moreover, cereal-grain legume mixed cropping is a strategy to improve agricultural sustainability. This study aimed to i) evaluate if soil variability could affect grain yield in barley-pea mixed cropping, and ii) improve the overall performance of barley-pea by modifying the relative cereal-legume crop densities.
Methods
Two field trials were performed to evaluate differential N fertilization effects on grain yield of barley-pea replacement design combinations. High and Low N inputs were designed by adjusting N levels based on barley density. Moreover, the 2017 trial let us to suppose the occurrence of an interaction between soil physical properties and N fertilization. Therefore, in 2018, based on a pedological survey subsequently evaluated by principal component analysis, the soil effect was included in ANOVA models applied to evaluate crop performance parameters.
Results
Nitrogen fertilization positively affected barley performance only in the soil unit located downslope, and a significantly lower presence of weeds was observed in mixed crops rather than in pea pure crops. Increasing the pea density from 50–80%, reducing the barley density (from 50–20%), and tailoring N fertilization were effective approaches to obtain a more balanced mixed grain harvest for animal feeding.
Conclusion
The combination of crop performance evaluation and assessments of soil conditions suggested that more sustainable agricultural systems based on within-crop biodiversity and a significant reduction of N fertilizers and herbicides can be achieved with barley-pea mixed cropping.
Comparison of Good Clinical Practice Inspection Processes for Marketing Applications between the United States Food and Drug Administration and the European Medicines Agency
Authors: Ayalew Kassa , Ning Yang-Min , Foringer Michelle J. , Leibenhaut Susan , Sellers Jenn , Yu Bei , Kronstein Phillip D. , Higgerson Agata , Mihaescu Camelia , Rodriguez Miguel , Williams LaKisha , Khin Ni A. 
Published: June 2022

Abstract
Background

The U.S. Food and Drug Administration (FDA) and European Medicines Agency (EMA) began collaboration on Good Clinical Practice (GCP) inspections for marketing applications since 2009. The main characteristics of the GCP inspection processes between FDA and EMA were never evaluated. This is the first analysis comparing the GCP inspection processes between the two agencies.

Methods

We examined and analyzed the key characteristics of the GCP inspection processes, including the geographical distribution, inspection types and timelines from application submission to final inspection reporting for marketing applications from September 2009 through December 2015.

Results

Fifty-five shared applications were included for analysis. For these applications, a total of 433 GCP inspections were conducted in 47 countries. Most CI inspections were conducted in regions outside of each agency’s own regulatory jurisdiction, while most sponsor/contract research organizations (CRO) inspections were conducted in the U.S. by both agencies. Twenty-eight shared applications included common sites inspected by both agencies. There were 15 joint inspections conducted for seven of these applications and the remaining applications had common sites inspected by both agencies at separate times. Of the joint inspections, 73% were conducted in the U.S and 20% in the E.U. The median time from submission of an application to generation of final inspection reports was 232 days for FDA and 204 days for EMA, with no significant differences noted among applications with and without common sites.

Conclusion

The inspection processes and timelines between the two agencies were similar, providing support for continued FDA-EMA GCP collaboration.
Consecutive Transcutaneous and Epidural Spinal Cord Neuromodulation to Restore Complete Paralysis
Authors: Mukhametova Elvira , Militskova Alena , Biktimirov Artur , Kharin Nikita , Semenova Elena , Sachenkov Oskar , Baltina Tatiana , Lavrov Igor 
Published: June 2022

Abstract
In this study the effect of transcutaneous (tSCS) and epidural electrical spinal cord stimulation (EES) to facilitate volitional movements, balance, and non-motor functions was consecutively tested in two subjects with motor complete spinal cord injury (SCI). We hypothesized that tSCS and EES have different but complimentary effects on restoration after SCI. In both subjects tSCS demonstrated minimal improvement in volitional movements critical to initiate tSCS-enabled rehabilitation. Compared to tSCS, following EES demonstrated immediate restoration of volitional movements, while tSCS was more effective in improving balance. Continuous improvement in non-motor functions was found during tSCS-enabled and then EES-enabled rehabilitation. These results show significant difference in effect of tSCS and EES on recovery of neurologic functions and demonstrate advantages of consecutive tSCS and EES therapy of SCI. Proposed approach will help to select responsive to neuromodulation subjects and early initiate therapy, particularly for motor complete SCI with minimal effect from conventional rehabilitation.
Multiple Sclerosis and health outcomes: an umbrella review of systematic reviews and meta-analyses
Authors: Shen Lianli , Zhong Lixian , Chen Sisi , Li Lichen , Tang shaohui 
Published: June 2022

Abstract
Purpose The previous analysis of systematic reviews and meta-analyses have illustrated that multiple sclerosis (MS) is correlated with multiple health outcomes. We designed an umbrella review to evaluate the breadth, validity and presence of biases of the associations of MS with diverse health outcomes. Methods We searched three databases (PubMed, We of Science and Embase) to recalculate the summary effect sizes, 95%CI, heterogeneity and small-study effects in the included meta-analyses. For each meta-analysis, we assessed the quality with AMSTAR2 and graded the epidemiologic evidence. Results A total of 28 articles comprising 84 unique meta-analyses were included in this study. Among the 84 unique outcomes, 52 unique outcomes had significant associations (p < 0.05). Only 6 outcomes (inflammatory bowel disease (IBD), ulcerative colitis (UC), crohn’s disease (CD), atrial fibrillation, herpes simplex virus type 2-IgG, hypothyroidism) showed high quality of epidemiological evidence. The remaining 46 outcomes were assessed to moderate or weak quality of evidence. Conclusion In our umbrella review, MS seems to increase the risk of IBD, UC, CD, herpes simplex virus type 2-IgG and hypothyroidism while decrease the risk of atrial fibrillation, with high epidemiological evidence. In order to verify our results, more prospective observational studies are needed.
A Supervised Learning Approach for Classification of Medical Image Retrieval
Authors: Battur Ranjana , N Jagadisha 
Published: June 2022

Abstract
The advancement in biomedical engineering have been significant to the medical or healthcare industry. However, it faces issues like how it can be applied to the medicine and biology for healthcare aspects. Recently, quick advances of programming and equipment innovation have made simple, the issue of keeping up beneficial images accumulations. Visual elements like shading, and shape and composition are actualized for image retrieval. Conventional strategies for image indexing have been demonstrated neither reasonable nor effective regarding space and time so it set off the advancement of the new approach. A new concept called Content-based image retrieval (CBIR) is beneficial for the different sort of medical images having dissimilar imaging modalities, anatomic areas with diverse directions and biological schemes is projected. Classification of the medical image retrieval is the major concern for group of medical image. Hence, support vector machine (SVM) classifier can be favorable for grouping forecast of query and database images based on similarity matching. It is very difficult to detect the features of the compared images effectively for all the different types of queries. Hence, the proposed SVM-MIR aims to classify and retrieval of biomedical images using SVM classifier method. The SVM-MIR based classification considers numerous groups of medical images for analysis. The outcomes of the proposed SVM-MIR approach achieve better performance compared to the existing approach.
Hypoxia-reprogramed megamitochondrion contacts and engulfs lysosome to mediate mitoselfphagy
Authors: Hao Tianshu , Yu Jianglong , Wu Zhida , Jiang Jie , Gong Long , Wang Bing-Jun , Zhao Huabin , Lu Bin , Engelender Simone , He He , Song Zhiyin 
Published: June 2022

Abstract
Mitochondria are the key organelles for sensing oxygen, which is consumed by oxidative phosphorylation to produce ATP to power the cell. Lysosomes contain hydrolytic enzymes that degrade misfolded proteins and damaged organelles, including mitochondria, to maintain cellular homeostasis. Mitochondria physically and functionally interact with lysosomes to regulate cellular metabolism. However, the mode and biological functions of mitochondria-lysosome communication remain largely unknown. Here, we show that hypoxia reshapes normal tubular mitochondria to megamitochondria by inducing wide inter-mitochondria contacts and subsequent fusion. Importantly, under hypoxia, mitochondria-lysosome contacts are promoted, and certain lysosomes are engulfed by megamitochondria, in a process we termed “megamitochondria engulfing lysosome (MMEL)”. Both megamitochondria and mature lysosome are required for MMEL. Moreover, we identified that the STX17-SNAP29-VAMP7 complex contributes to mitochondria-lysosome contacts and MMEL under hypoxia. Intriguingly, MMEL and mitochondrial proteases, which are activated by hypoxia, mediate a new mode of mitochondrial degradation, which we named “mitoselfphagy”. Unlike mitophagy, mitoselfphagy degrades mitochondrial proteins unevenly in a manner independent of the autophagosome. Additionally, mitoselfphagy increases mitochondrial ROS production. Our findings reveal a novel mode of crosstalk between mitochondria and lysosomes and uncover a new pathway of mitochondrial quality control.
Protective Effect of Thyme Leaf on Metabolic Changes Induced by Monosodium Glutamate
Authors: Dusak Zeynep Bozkoyun , Atasoy Nurhayat , Kömüroğlu Ahmet Ufuk 
Published: June 2022

Abstract
Monosodium glutamate (MSG) is widely used as a food additive, and there is a growing concern that excitotoxins such as MSG play an important role in the development of metabolic disorders. Therefore, studies on MSG are increasing day by day. In this study, the protective effect of thyme on the metabolic syndrome caused by MSG was investigated. Thirty-two Wistar albino rats were divided into 4 groups, 8 in each group, as a control group, thyme group (250 mg/kg), MSG group (2g/kg), and MSG + thyme group. At the end of the 28-day trial, the rats were sacrificed and their blood was taken. The blood was centrifuged and the serum was separated. AST, ALT, LDH, ALP, glucose, insulin, urea, creatinine, total protein, albumin levels of serums, and 8-OHdG levels were measured in the Van YYU Faculty of Medicine Biochemistry laboratory using Abbott brand commercial kits. In addition, liver and kidney tissues were examined with only hispatological tests. Compared to the control group, the serum glucose level increased significantly in the MSG group. The serum glucose level of the MSG + thyme group decreased significantly from the MSG group. There was a slight increase in serum AST and ALT levels in the MSG group. As a result, it was concluded that the administration of MSG may have disrupted the carbohydrate metabolism by increasing the serum glucose level significantly. It was observed in the study that the thyme plant could cause this effect to decrease.
Simulated digestion of the pigmented legumes’ (black chickpea (Cicer arietinum L.) and brown lentil (Lens culinaris Medikus)) phenolics to estimate their bioavailability
Authors: Kose Bengi Ece , Tacer-Caba Zeynep , Nilufer-Erdil Dilara 
Published: June 2022

Abstract
This study simulated the gastrointestinal digestions of black chickpea (BC) and brown lentil (BL). BC phenolics were better retained (an increase of 35.1%) than the BL phenolics (a loss of 7.4%) after cooking. In contrast, the remaining flavonoids after cooking were higher in BL (about 47%). TAA (Total antioxidant activity) of free fractions increased in BC and BL samples by cooking. After in vitro GID, moderate levels of flavonoids were detected in colon (OUT) fractions. TAA levels correlated well with total flavonoids. TAA by the CUPRAC assay was higher in cooked and in vitro GID BC and BL samples. Catechin was the most abundant compound detected in BC samples, while gallic acid was for BL. BC and BL, have unique and superior benefits for health when compared with the conventional legumes. The possible interactions between their remaining phenolics and other bioactives in the colon are promising for their widespread consumption.
Deep Learning Driven Adaptive Optics for Single Molecule Localization Microscopy
Authors: Huang Fang , Zhang Peiyi , Ma Donghan , Cheng Xi , Tsai Andy , Tang Yu , Gao Hao-Cheng , Fang Li , Bi Cheng , Landreth Gary , Chubykin Alexander 
Published: June 2022

Abstract
The inhomogeneous refractive indices of biological tissues blur and distort single molecule emission patterns generating image artifacts and decreasing the achievable resolution of single molecule localization microscopy (SMLM). To compensate tissue induced aberrations, conventional sensorless adaptive optics methods rely on iterative mirror-changes and image-quality metrics. However, these metrics result in inconsistent, and sometimes opposite, metric responses and thus fundamentally limit the efficacy of these approaches for aberration correction in tissues. Bypassing the previous iterative trial-then-evaluate processes, we developed deep learning driven adaptive optics for SMLM to allow direct inference of wavefront distortion and near real-time compensation. Our trained deep neural network monitors the individual emission patterns from single molecule experiments, infers their shared wavefront distortion, feeds the estimates through a dynamic filter, and drives a deformable mirror to compensate sample induced aberrations. We demonstrated that our method simultaneously estimates and compensates 28 types of wavefront deformation shapes, restores single molecule emission patterns approaching the conditions untouched by the specimen, and improves the resolution and fidelity of 3D SMLM through >130 µm thick tissue specimens, with as few as 3-20 mirror changes.
Measurement of Adherence and Health-related Quality of Life during Anticoagulation Therapy in Cancer Associated-Venous Thromboembolism (VTE): A Multicenter Quantitative Study
Authors: Font Carme , Gomez-Mesa Juan Esteban , López-Núñez Juan Jose , Calderón Caterina , Galindo-Coral Stephania , Wu Carol C. , Ma Junsheng , Kroll Michael H. , Rojas-Hernandez Cristhiam Mauricio 
Published: June 2022

Abstract
Purpose
Therapy for cancer associated-venous thromboembolism (VTE) includes long term anticoagulation, which may have substantial impact on the health-related quality of life (HRQL) of patients. We assessed patient-reported outcomes to characterize the HRQL associated with VTE treatment and to begin to examine those HRQL elements impacting anticoagulation adherence (AA).
Methods
Participants were adult cancer patients with confirmed symptomatic acute lower extremity deep venous thrombosis. Patients were excluded if there was an indication for anticoagulation other than VTE, ECOG performance status > 3 or life expectancy < 3 months. Participants were assessed with a self-reported adherence tool. HRQL was measured with a 6-domain questionnaire using a seven-point Likert scale. Evaluations were performed at 30 days and 3 months after enrollment. For the primary objective, an overall adherence rate was calculated at each time point of evaluation. For the HRQL domains, non-parametric testing was used to compare results between subgroups.
Results
74 patients were enrolled. AA and HRQL at 30 days and 3 months were assessed in 50 and 36 participants, respectively. At 30 days the AA rate was 90% and at 3 months it was 83%. In regard to HRQL, patients suffered frequent and moderate-severe distress in the domains of emotional and physical symptoms, sleep disturbance and limitations to physical activity. An association between emotional or physical distress and AA was observed.
Conclusion
Patients with VTE suffer a substantial impairment of their HRQL. Increased distress correlated with better AA. These results can be used to inform additional research aimed at developing novel strategies to improve AA.
IoT edge based framework for sybil and buffer overflow detection
Authors: Pandey Utpal , Chaurasia Brijesh Kumar 
Published: June 2022

Abstract
The Internet of Things (IoT) is gradually spreading across the globe, offering a wide variety of opportunities across every part of our lives. Unfortunately, the Internet of Things comes with several information technology flaws and exploits. As the number of security vulnerabilities continues to rise, cybersecurity remains a serious problem for every business in cyberspace. In recent times thousands of zero-day attacks are known to exist. As a result, the addition of new attacks emerges regularly several protocols, mostly from the IoT. There are minor modifications to previously reported cyberattacks. This suggests that even advanced mechanisms like basic machine learning systems have a hard time identifying anomalies. IoT applications pose various threats to businesses, according to security experts. Because of the widespread acceptance of IoT devices, their variety, standardization challenges, and inherent versatility, businesses need an intelligent mechanism capable of the detecting unauthorized IoT devices automatically and connected to their networks. In this study, a machine learning approach is used to identify unauthorized IoT devices by detection of sybil and buffer overflow attack among IoT devices infrastructure. The CNN technique focuses on detecting the attack or activity of any malicious node, as well as attempting to resolve the problem. In this research, CNN, a deep learning technique, was applied for extracting features from the network traffic data to accurately identify malicious IoT devices.
Polarization-independent graphene-based electro-absorption modulator using semi-ellipsoid HfO2 slot waveguide
Authors: Dalvand Hanye , Nikoufard Mahmoud , Zangeneh Hamid Reza 
Published: June 2022

Abstract
In this article, a wideband polarization-independent graphene based electro-absorption modulator (EAM) operating in the wavelength range of 1400 nm–2000 nm is proposed. The proposed modulator consists of a slot waveguide comprising two semi-ellipsoid hafnia (HfO2) layers with a slot width of 100 nm embedded in a silicon dioxide (SiO2) substrate, caped with a graphene layer, and two electrodes on both sides. The key advantages of this structure include improvements at a wavelength of 1550 nm in polarization-sensitive loss (PSL ≤ 0.11 dB), propagation length of 6170 µm, 3dB bandwidth of 500 GHz, mode power attenuation of 7×104 dB/m, and insertion loss of -23.22 dB/µm. Moreover, the effects of wavelength on PSL and radius on extinction ratio were investigated, resulting in tunable PSLs in “OFF” and “ON” states at a wavelength of 1550 nm. Thus, the proposed EAM can be potentially utilized in electro-optical (EO) switching.
Spontaneous ventral hernia through the rectus abdominis muscles: a case report
Authors: washiro mitsutsune Benedict , Kitahara Takuya , Yanagisawa Nobuo , Narita Jun , Danzuka Takao , Ohtsuka Masayuki 
Published: June 2022

Abstract
Background A hernia is a condition in which tissues or organs are abnormally protruding through a defect in the surrounding wall. We experienced a rare spontaneous ventral hernia that penetrated the right transverse abdominal fascia and rectus abdominis muscles. Case presentation A 92-year-old woman was admitted to our hospital with major complaints of right abdominal pain and some quadrant mass. She had no history of trauma and lower abdominal surgery. Computed tomography revealed that a protrusion of the hernia from the right lower abdominal wall. The hernia contents were small intestine, which penetrated the transverse abdominis fascia and rectus abdominis muscles. The herniation has been manually reduced. Elective surgery was performed 3 days later. The intraoperative findings indicated that the hernia sac was located around the middle of the rectus abdominis muscles in the right lower abdominal wall. Hernioplasty was performed using Bard Light PerFix Plug®. The patient fully recovered and was discharged 8 days after surgery. No recurrence was identified for 42 months during patient follow-up. Conclusions We presented a rare case of hernia, which was protruded through the rectus abdominis muscles. Intrinsic anatomical weakness of the abdominal wall and age-related muscle atrophy might have caused this rare hernia. This case report provides clinicians useful information for accurate diagnosis and successful treatments of this type of hernia in the elderly, and contributes to identifying the anatomical features of some rare ventral hernias.