bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Functional Cotton Fabric by Simultaneous Dyeing and Finishing with Novel Bioactive Reactive dye
Authors: Nosheen Anum , Ashraf Munir , Hussain Muhammad Tahir , Rehman Abdur , Aziz Humera , Riaz Shagufta , Akram Saba 
Published: July 2022

Abstract
Bioactive textiles have garnered considerable attention during the current pandemic COVID-19 owing to their antimicrobial properties. However, the fabrication and utilization of bioactive textiles have imposed a toxic impact on the environment due to the leaching of bioactive agents during laundering. In this study, a novel functional reactive dye was synthesized with an antibacterial agent covalently linked to the triazine reactive group for simultaneous dyeing and finishing of cotton fabric. The synthesized dye was characterized by FTIR, 1H-NMR, 13C-NMR, and UV-Vis spectroscopy for structure elucidation and confirmation. The dye was applied to cotton fabric using the exhaust dyeing method. The antibacterial efficacy of the powder dye and dyed fabric against Staphylococcus Aureus and Escherichia Coli was estimated according to standard test protocols. The color strength (K/S), colorimetric data (L*, a*, b*, C*, H*) measurement, ultraviolet protection, and colorfastness properties of dyed fabric were also established and evaluated. The synthesized powder dye revealed outstanding antibacterial activity and killed 99.99% of bacteria (E. coli and S. aureus). The dyed fabric showed excellent antibacterial activity before washing and after 20 industrial laundry cycles, there was no significant reduction in antibacterial activity establishing non-leaching of antibacterial agent into environment. The dyed fabrics before and after washing showed excellent ultraviolet protection values (> 50), better dye fixation, and fastness properties. The current study has established a sustainable process to develop bioactive textiles which do not leach bioactive agent in the environment, and it is easily scalable at industrial scale.
Anti-hyperuricemia mechanism of TongFengTangSan (TFTS) by UHPLC-Q-TOF/MS-based nontargeted metabonomics
Authors: Huang Zhichao , Zhang Wugang , An Qiong , Lang Yifan , Liu Ye , Fan Huifang , Chen Haifang 
Published: July 2022

Abstract
Background: TongFengTangSan (TFTS) is a commonly used Tibetan prescription for the treatment of gout. Previously, we confirmed that 90% alcohol extract of TFTS (CF) has a significant uric acid-lowering effect; however, the anti-hyperuricemia mechanisms and main active fractions remain unclear. This study aimed to investigate the anti-hyperuricemia mechanism by metabolomics and confirm the active fraction of CF. Methods: Hyperuricemia model was established by intraperitoneal injection of 100 mg/kg potassium oxonate and 150 mg/kg hypoxanthine by gavage. The serum uric acid (sUA), creatinine (CRE), blood urea nitrogen (BUN), xanthine oxidation (XOD) activity, interleukin-6 (IL-6) and interleukin-1β (IL-1β) were used as indicators to evaluate the efficacy of the CF and four fractions (SX, CF30, CF60, and CF90). The anti-hyperuricemia mechanism of CF in the treatment of hyperuricemia was evaluated through nontargeted metabolomics based on UPLC-Q-TOF-MS technology. Principle component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to explore the potential biomarkers in hyperuricemia, as well as the differential metabolites and metabolic pathways regulated by CF and four fractions.Results: CF showed the significant anti-hyperuricemia effect through the down-regulation on the level of sUA, sCRE, sIL-1β and XOD. SX, CF30, CF60, and CF90 showed the difference on the anti-hyperuricemia effect. Among four fractions, CF60 significantly lowered the level of sUA and could be the main lowering uric acid efficacy fraction of TFTS. 33 differential metabolites were identified in hyperuricemia rats. Pathway analysis revealed five pathways disrupted in hyperuricemia rats. CF reversed 12 endogenous metabolites and the anti-hyperuricemia mechanism of CF could involve in the regulation on purine metabolism and riboflavin metabolism. SX, CF30, CF60, and CF90 regulated 6, 6, 10 and 5 differential metabolites, and involved in differential metabolic pathways, respectively. Among these metabolic pathways, CF60 regulate purine metabolism and riboflavin metabolism. In addition, CF and CF60 could improve intestinal and renal function through the reverse of abnormality of purine metabolism. Conclusions: CF60 could be the main active fraction of CF. CF and CF60 could exert anti-hyperuricemia effect through the regulation on the abnormal purine metabolism caused by hyperuricemia and consequently the improvement of intestinal and renal function.
Knockdown of long noncoding RNA SAN rejuvenates aged adipose-derived mesenchymal stem cells via miR-143-3p/ADD3 axis
Authors: Xiong Hewei , Ren Sen , Chen Jing , Yang Xiaofan , Liu Yutian , Xu Zhao , Guo Jiahe , Jiang Tao , Yuan Meng , Liu Yang , Zhang Guolei , Li Wenqing , Machens Hans-Günther , Chen Zhenbing 
Published: July 2022

Abstract
Background
Senescent adipose-derived stem cells (ASCs) exhibit reduced therapeutic efficacy during wound healing. Transcriptional regulation factors including long non-coding RNAs (lncRNAs) reportedly play essential roles in stem cell aging. However, the mechanisms by which lncRNAs influence mesenchymal stem cell aging have not been investigated.
Methods
The lncRNA senescence-associated non-coding RNA (SAN) was identified among differentially expressed lncRNAs in ASCs obtained from old and young volunteer donors. ASCs with overexpression or knockdown of SAN and ADD3 were constructed by lentiviral transduction. Proliferation, migration, and cellular senescence were investigated in ASCs, as were the angiogenic effects on endothelial cells and promigratory effects on fibroblasts stimulated with conditioned medium (CM) from ASCs. Dual-luciferase assays were performed to characterize RNA interactions. The therapeutic effects of SAN-depleted aged ASCs were evaluated in a skin injury model.
Results
The lncRNA SAN (NONHSAT035482.2) was upregulated in aged ASCs and functioned to regulate cellular senescence in ASCs. lncRNA SAN knockdown and ADD3 silencing in ASCs led to ASC functional enhancement and the inhibition of cellular senescence; it also promoted the effects of CM on endothelial cells and fibroblasts. Mechanistic analysis showed that SAN serves as a sponge for miR-143-3p, thereby regulating the expression of γ-adducin (ADD3); moreover, ADD3 silencing could rejuvenate senescent ASCs. The application of SAN-depleted aged ASCs accelerated the skin wound closure, compared with transplantation of aged ASCs.
Conclusion
The lncRNA SAN mediates ASC senescence by regulating the miR-143-3p/ADD3 pathway, providing a potential target for rejuvenation of senescent ASCs and enhancement of wound repair.
Knowledge, attitude and practice of healthcare providers on mistreatment during labour and childbirth: a cross-sectional study in Tehran, Iran, 2021
Authors: Mirzania Marjan , Shakibazadeh Elham , Bohren Meghan A. , Babaey Farah , Hantoushzadeh Sedigheh , Khajavi Abdoljavad , Foroushani Abbas Rahimi 
Published: July 2022

Abstract
Background: Mistreatment of women during childbirth is a global health challenge. Maternity healthcare providers have key roles in influencing women’s birth experiences. This study aimed to assess knowledge, attitudes and practices of maternity healthcare providers about mistreatment during labour and childbirth in public hospitals in Tehran, Iran.Methods: This cross-sectional study was conducted from October to December 2021 in five public hospitals in Tehran. All maternity healthcare providers (obstetricians, midwives) and students were invited to the study. Data were collected using a questionnaire consisting of four sections: socio-demographic characteristics (11 items), knowledge (11 items), attitudes (13 items), and practices (14 items) about mistreatment. Knowledge, attitude, and practice levels were determined using Bloom’s cut off point. Data were analyzed using descriptive and analytical statistics at a significant level of p < 0.05.Results: A total of 255 individuals participated (response rate: 94.5%). Most participants (82.7%) had poor knowledge about physical abuse, verbal abuse, poor rapport between women and providers, and failure to meet professional standards of care. Most participants (69.4%) were alright with physical abuse, verbal abuse, and discrimination. Self-reported practices of different types of mistreatment were not common and only 3.1% of the participants were in moderate level. However, shouted at women, applied fundal pressure, and slapped the thighs during birth were categories used by providers. Age, profession, field of study, employment status, monthly income, work experience, numbers of night shifts, and training history were significantly related with the participants’ knowledge, attitudes, and practices about mistreatment.Conclusions: Knowledge, attitudes, and practices of our participants were poor about maternity mistreatment. Findings of our study have important implications for program planners and decision makers on development of effective interventions to reduce mistreatment during labour and childbirth in Iran. These interventions should include designing and implementing continuing education courses and revising the educational curriculums to increase knowledge, strengthen positive attitudes, and modify practice of maternity healthcare providers, overcoming staff shortages, paying staff fairly, establishing support culture for mother-centered and respectful care, and increasing quality of maternity care.
Active site and structural analysis of sesquiterpene synthase towards synthesis of aromatic compounds related to agarwood formation in Aquilaria malaccensis
Authors: Aminan Aimi Wahidah , Hamid Azzmer Azzar Abdul , Tajuddin Saiful Nizam , Ramli Aizi Nor Mazila 
Published: July 2022

Abstract
Agarwood is a fragrant resinous wood that has been widely used in traditional medicines, incense and perfume. Formation of agarwood in branches and trunks of certain genera including Aquilaria and Gyrinops is due to activation of defence mechanism that causes by natural phenomena or microbial infection. The main fragrant compounds of agarwood are sesquiterpenes and phenylethyl chromone derivatives, and a great variety of sesquiterpenes are contained in high-quality agarwood. Their diverse cyclic and acyclic structures are known to be synthesized from farnesyl pyrophosphate (FPP) by sesquiterpene synthase catalytic action. Mechanisms of reaction control and structures of sesquiterpene synthases from Aquilaria sp are studied by many groups trying to clarify the structure-function relationship in these enzymes, however the molecular recognition between the catalytic residues of sesquiterpene synthases with FPP producing the aroma compound remains unclear. In this study, sesquiterpene synthases isolated from Aquilaria malaccensis (AGS3) was predicted for its structure and docking simulation has been performed to determine the affinity of active site residues of sesquiterpene synthases towards FPP molecules. To reveal the unique catalytic mechanism of AGS3, the molecular docking of AGS3 was carried out in two different sites involving active site entrance for initial catalysis and within substrate cavity for final step. It was found that the aspartate and glutamate rich regions interacts with metal complexes at the entrance of active site and aromatic residues was subsequently interact with farnesyl chain of FPP within the substrate binding pocket while the diphosphate moiety of FPP (ppi) detached the substrate. This model will serve to be a framework for exploring the roles of active site residues of AGS3 to explore the enzyme function of sesquiterpene synthase producing aromatic compound sesquiterpene of agarwood in A. malaccensis.
Chromatic Polynomial of Intuitionistic Fuzzy Graphs(IFGs) Using α-Levels
Authors: Hordofa Lateram Zawuga , Repa V.N.SrinivasaRao , Ashebo Mamo Abebe , T Nageswara Rao 
Published: July 2022

Abstract
Objective: In graph coloring, determining the chromatic polynomial is exactly finding the number of alternative solutions anywhere graph coloring is applied. In this article, We use α level to compute the chromatic polynomial of the Intuitionistic fuzzy graph. In addition, We convert (α,β) level to α level and define α level graph. Moreover, We compute the chromatic polynomial for the different α level graphs with illustrative examples.Results: (α,β)- level to α-level conversion method is developed, and based on that α level graph is defined. Besides, the different α level graphs are computed with illustrative examples. In addition, certain properties of α- level graphs and their chromatic polynomials are presented. Moreover, the chromatic number, the number of vertices, the number of edges, and the chromatic polynomial of the different α-level graphs are compared.Mathematics Subject Classification: Primary: 05C72, 05C31. Secondary: 03B20, 03E72.
Clinical profile, treatment, histopathological, immunohistochemical, and molecular analyses of pilocytic astrocytoma- an eight year study from a tertiary health care centre in North East India
Authors: Ahmed Shabnam Akhtar , Barua Navanil , Borah Nabajyoti , Haque Inamul , Barman Ananya , Dandapath Iman 
Published: July 2022

Abstract
Purpose: Pilocytic astrocytoma (PA) is a central nervous system (CNS) World Health Organization (WHO) grade 1 glial tumor that is highly prevalent in children and young adults. The main aim of the study was to assess the frequency, clinicopathological features and treatment of PAs along with their immunohistochemical and molecular analyses. Methods: Approximately, 144 glial tumors were diagnosed in patients from 3 to 75 yrs of age from January 2015 to March 2022. The tumors were classified according to 2021 CNS WHO classification. Nine pediatric PA patients were identified and their clinical data analyzed. Immunohistochemistry (IHC), fluorescence in-situ hybridization (FISH) and molecular analysis using the real time polymerase chain reaction (RT-PCR) were performed for the PAs. Standard deviations were calculated using the Microsoft excel for statistical validations. Results: The mean age of the glioma patients was 41.7 yrs ± 18.2 with a male/female ratio of 1.3:1. The most common form of glial tumor was found to be astrocytoma CNS WHO grade 2 (31.9 %). Other frequently occurring tumors were astrocytoma CNS WHO grade 3 (29.2 %), astrocytoma CNS WHO grade 4 (13.9 %), diffuse astrocytoma CNS WHO grade 2 (7.4 %), PA CNS WHO grade 1 (6.9 %), and oligodendroglioma CNS WHO grade 3 (4.2 %). The pediatric PA cohort had a mean age of 9.2 yrs ± 4.9 with a male/female ratio of 2:1. Glial fibrillary acidic protein (GFAP) positive immunostaining and retention of transcriptional regulator ATRX expression was seen in all the tested PAs. None of the PAs showed isocitrate dehydrogenase (IDH1), MIB1, and tumor protein p53 expression. The KIAA1459-BRAF fusion was detected in four PAs. Surgical intervention with total or radical tumor excision was performed for the PA patients. Most PA patients exhibited improved condition post-surgery. Conclusion: With the advent of healthcare and newer diagnostic facilities there is increased incidence of glial tumors in developing countries. Combination of histological, immunohistochemical, and molecular analysis is very important for the diagnosis, accurate treatment, and prognosis of PA patients.
Asellus aquaticus removal of unfertilized fish eggs and possible use in aquaculture as a biological control organism
Authors: Kucska Balázs , Ngọc Quyến Nguyễn , Havasi Máté , Staszny Ádám , Ivánovics Bence , Vranovics Károly , Griffitts Jeffrey Daniel , Urbányi Béla , Müller Tamás 
Published: July 2022

Abstract
The aim of the present experimental study was to better understand the foraging behavior of Asellus aquaticus. Different numbers of A. aquaticus were introduced into different experimental setups of unfertilised eggs, viable eggs, hatched larvae of Danio rerio. The number and time of A. aquaticus significantly affected the ratio of consumed non-fertilised eggs in each experimental cycle (MANOVA, p<0.05). A. aquaticus belongs to the aquatic saprophytes and no predatory behavior was observed during the experiments. They were able to distinguish between the dead eggs and those containing living embryos. Additionally, zebrafish larvae were not harmed by the A. aquaticus, even when there was not an alternative food source. The results help to understand the potential sanitary role of these crustaceans in natural waters and provide new insight into their possible application as a biological control organism in aquaculture hatcheries. Finally, our results indicate that there is a potential for A. aquaticus application against pathogens by reducing bacterial and fungal growth substrates.
Effect of religious service attendance on mortality and hospitalisations among Danish men and women: longitudinal findings from REGLINK-SHAREDK
Authors: Ahrenfeldt Linda Juel , Möller Sören , Hvidt Niels Christian , VanderWeele Tyler J. , Stripp Tobias Kvist 
Published: July 2022

Abstract
Background: Research suggests a protective effect of religious service attendance on various health outcomes. However, most research has been done in religious societies, raising the question of whether these associations are also prominent in secular cultures. Here we examine mortality and hospitalisations by religious service attendance among men and women in a secular society. Methods: We performed a cohort study including 2987 Danes aged 40+ interviewed in SHARE from 2004-2007, whom we followed up in the Danish registries until 2018. We used Cox regressions and negative binomial regressions to examine associations, including interactions with sex and adjusting for age, wave, socioeconomic factors, lifestyle factors, body mass index, and history of diseases. Results: Overall, 5.0% of men and 6.6% of women reported that they took part in a religious organisation within the last month. Among 848 deaths, we found lower overall mortality for people who attended religious services (hazard ratio (HR) 0.70; 95% CI 0.50-0.99). There was evidence for association among women (HR 0.56; 95% CI 0.35-0.89), but not among men (HR 0.95; 95% CI 0.59-1.53). In contrast, regarding hospital admissions (n = 12,010), we found lower hospitalisation rates among men who attended religious services (incidence rate ratio (IRR) 0.67; 95% CI 0.45-0.98), whereas no association was found among women (IRR 0.95; 95% CI 0.70-1.29). Sensitivity analyses with e-values were moderately robust.Conclusions: Our results contribute to the limited literature on possible health benefits of religious service attendance in secular societies, demonstrating lower mortality among women and fewer hospitalisations among men.
Hybrid prediction modeling for residual stress profile induced by multi-axis milling of Ti-6Al-4V titanium alloy combined finite element with experiment
Authors: Wang Zongyuan , Zhou Jinhua , Ren Junxue , Shu Ailing 
Published: July 2022

Abstract
Complex curved thin-walled structures, mainly using multi-axis milling, are highly susceptible to deformation induced by residual stress. It is therefore that there is a considerable amount of researches on developing predictive models for machining-induced residual stress. However, these developed models for residual stress prediction mainly focus on turning and three-axis milling. For multi-axis milling, a hybrid model combining experimental results and finite element(FE) model is established to predict the residual stress profile of Ti-6Al-4V titanium alloy in the current study. Based on the experimental and simulated results, the residual stress profile is fitted by the hyperbolic tangent function using the firefly algorithm (FA). Good fitting accuracy is obtained, which the R2 values change from 85.3–99.1% in the σx direction and change from 80.7–98.1% in the σy direction. The radial basis function (RBF) neural network is used to establish the relationship between the coefficients of hyperbolic tangent model and the milling parameters. The prediction accuracy is verified to achieve 92.7% and 91.4% in the σx and σy directions, respectively. The effects of cutting speed, feed rate, and inclination angle on surface residual stress and influence depth are investigated. The results show that there is a strong nonlinear relationship between the surface residual stress and milling parameters. The proposed hybrid prediction model of residual stress can be used for further machining optimization of complex curved thin-walled structures.
Simulating Large PEPs Tensor Networks on Small Quantum Devices
Authors: MacCormack Ian , Galda Alexey , Lyon Adam 
Published: July 2022

Abstract
We systematically map low-bond-dimension PEPs tensor networks to quantum circuits. By measuring and reusing qubits, we demonstrate that a simulation of an N x M square-lattice PEPs network, for arbitrary M, of bond dimension 2 can be performed using N + 2 qubits. We employ this approach to calculate the values of a long-range loop observable in the topological Wen plaquette model by mapping a 3 x 3 PEPs tensor network to a 5-qubit quantum circuit and executing it on the Honeywell System Model H1-1 trapped-ion device. We find that, for this system size, the noisy observable values are sufficient for observing the phase transition from topological to trivial order, as the Wen model is perturbed by a magnetic field term in the Hamiltonian. Our results serve as a proof-of-concept of the utility of the measure-and-reuse approach for simulating large two-dimensional quantum systems on small quantum devices.
Electric-Inductive-Interdigital Capacitor Array MIMO Antenna with Enhanced Isolation by Metamaterial for Wi-Fi 5 &amp; 6E Applications
Authors: Kanakavalli Harshasri , R Pandeeswari 
Published: July 2022

Abstract
In this paper, two-port Electric-Inductive-Interdigital Capacitor (ELIDC) metamaterial Array Multi-Input-Multi-Output (MIMO) antenna is designed with enhanced gain, bandwidth, and isolation for frequency bands 5.125GHz – 5.825GHz and 5.925GHz-7.125GHz for the Wi-Fi 5 and 6E applications. The proposed MIMO antenna comprises corporate fed ELIDC metamaterial arrays, which are fed in two opposite directions. The ground plane consists of a Defected Ground Structure (DGS) for enhancement of bandwidth. A novel four split-ring resonator (SRR) array is embedded between two radiating elements of the DGS ground plane to increase the isolation. To validate the blocking of electromagnetic propagation between antenna elements, negative permeability in the desired frequency is extracted using the Nicolson-Ross-Weir (NRW) method. The isolation is enhanced to -30dB by incorporating the SRR array. The proposed ELIDC array MIMO antenna is fabricated and measured S parameters, gain and radiation pattern are giving good consistency between simulated results. The Envelope Correlation Coefficient (ECC), Diversity Gain (DG), and Total Active Reflection Coefficient (TARC) of the diversity performance show that the proposed ELIDC array MIMO antenna can be used in MIMO Tx/Rx systems.