bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary biliary cholangitis first demonstrated in pregnancy: a case report
Authors: Melo Daniela , Areia Ana Luísa , Moura Paulo 
Published: January 2022

Abstract
Background
We present a case of primary biliary cholangitis diagnosed during pregnancy. Diagnosis of this entity in pregnancy is infrequent, and when everything seemed to point to a simple obstetric cholestasis, close attention to the details of the clinical history was required to raise suspicion of the true diagnosis.

Case presentation
We present a 37-year-old Portuguese Caucasian patient who complained of generalized pruritus and showed alteration in hepatic function tests with a cholestatic pattern. The first diagnostic hypothesis was intrahepatic cholestasis of pregnancy, and she began treatment with ursodeoxycholic acid, which resulted in slight improvement of cholestasis. Her pregnancy was also complicated with occlusive hemorrhagic placenta, and at 30 weeks she underwent emergency cesarean section due to heavy blood loss. However, careful observation of clinical and laboratory findings, postpartum evolution, and a multidisciplinary approach to the patient led to the probable diagnosis of primary biliary cholangitis.

Conclusions
Physiological changes during pregnancy can mimic chronic liver disease that can only be revealed at this stage, having an impact not only on the pregnancy but on the entire future of the woman.
Promise and challenges of clinical non-invasive T-cell tracking in the era of cancer immunotherapy
Authors: Gosmann Dario , Russelli Lisa , Weber Wolfgang A. , Schwaiger Markus , Krackhardt Angela M. , D’Alessandria Calogero 
Published: January 2022

AbstractIn the last decades, our understanding of the role of the immune system in cancer has significantly improved and led to the discovery of new immunotherapeutic targets and tools, which boosted the advances in cancer immunotherapy to fight a growing number of malignancies. Approved immunotherapeutic approaches are currently mainly based on immune checkpoint inhibitors, antibody-derived targeted therapies, or cell-based immunotherapies. In essence, these therapies induce or enhance the infiltration and function of tumor-reactive T cells within the tumors, ideally resulting in complete tumor eradication. While the clinical application of immunotherapies has shown great promise, these therapies are often accompanied either by a variety of side effects as well as partial or complete unresponsiveness of a number of patients. Since different stages of disease progression elicit different local and systemic immune responses, the ability to longitudinally interrogate the migration and expansion of immune cells, especially T cells, throughout the whole body might greatly facilitate disease characterization and understanding. Furthermore, it can serve as a tool to guide development as well as selection of appropriate treatment regiments. This review provides an overview about a variety of immune-imaging tools available to characterize and study T-cell responses induced by anti-cancer immunotherapy. Moreover, challenges are discussed that must be taken into account and overcome to use immune-imaging tools as predictive and surrogate markers to enhance assessment and successful application of immunotherapies.
Combinatorial nanococktails via self-assembling lipid prodrugs for synergistically overcoming drug resistance and effective cancer therapy
Authors: Li Tongyu , Shi Weiwei , Yao Jie , Hu Jingyun , Sun Qiong , Meng Jing , Wan Jian , Song Haihan , Wang Hangxiang 
Published: January 2022

Abstract

Background
Combinatorial systemic chemotherapy is a powerful treatment paradigm against cancer, but it is fraught with problems due to the emergence of chemoresistance and additive systemic toxicity. In addition, coadministration of individual drugs suffers from uncontrollable pharmacokinetics and biodistribution, resulting in suboptimal combination synergy.

Methods
Toward the goal of addressing these unmet medical issues, we describe a unique strategy to integrate multiple structurally disparate drugs into a self-assembling nanococktail platform. Conjugation of a polyunsaturated fatty acid (e.g., linoleic acid) with two chemotherapies generated prodrug entities that were miscible with tunable drug ratios for aqueous self-assembly. In vitro and in vivo assays were performed to investigate the mechanism of combinatorial nanococktails in mitigating chemoresistance and the efficacy of nanotherapy.

Results
The coassembled nanoparticle cocktails were feasibly fabricated and further refined with an amphiphilic matrix to form a systemically injectable and PEGylated nanomedicine with minimal excipients. The drug ratio incorporated into the nanococktails was optimized and carefully examined in lung cancer cells to maximize therapeutic synergy. Mechanistically, subjugated resistance by nanococktail therapy was achieved through the altered cellular uptake pathway and compromised DNA repair via the ATM/Chk2/p53 cascade. In mice harboring cisplatin-resistant lung tumor xenografts, administration of the nanococktail outperformed free drug combinations in terms of antitumor efficacy and drug tolerability.

Conclusion
Overall, our study provides a facile and cost-effective approach for the generation of cytotoxic nanoparticles to synergistically treat chemoresistant cancers.
Ultrasonographic and macroscopic study of pregnancy in golden hamster
Authors: Keshavarz Mohammad Amin , Mogheiseh Asghar , Ahrari-Khafi Mohammad Saeed , Mahboobi Reza 
Published: January 2022

Abstract
Background
Hamster is widely used as an experimental model in the study of reproductive system. However, pregnancy diagnosis and aging always have been a challenge. ultrasonography have been used in diagnosis of pregnancy in some small laboratory animals, such as rabbits, rats, and mice. Current study describes use of trans-abdominal ultrasonography for pregnancy diagnosis and fetal age estimation in golden hamster. Furthermore, a macroscopic examination was performed to evaluate the embryonic vesicle diameter, crown-rump length, and fetal head diameter. Ten adult female golden hamsters were selected and maintained under controlled light conditions (14 h light/10 h darkness). The estrous cycle was synchronized using eCG and hCG. During estrous (18 h after hCG injection), the hamsters were naturally mated. After seven days of mating, the hamsters were examined daily for pregnancy diagnosis and aging with an ultrasound scanner equipped with an 8.5-MHZ linear probe. On each day of the experiment, at least one of the pregnant hamsters was euthanized and dissected for macroscopic fetal measurements using a digital caliper.

Results
The gestational sac and crown-rump length were identified and measured by ultrasonographicly on day 7 of pregnancy and head could be visible after day 10 of gestation. Statistical analysis revealed that the ultrasound estimation of gestational age was significantly correlated with the actual age of the fetus (r = 0.98; p < 0.05).

Conclusions
Real-time ultrasound can be used for the diagnosis of pregnancy and estimation of fetal age in golden hamster from day 7 of gestation.
Fault enhancement comparison among coherence enhancement, probabilistic neural networks, and convolutional neural networks in the Taranaki Basin area, New Zealand
Authors: Mora José P. , Bedle Heather , Marfurt Kurt J. 
Published: January 2022

Fault identification is a critical component of seismic interpretation. During the past 25 years, coherence, curvature, and other seismic attributes sensitive to faults improved seismic interpretation, but human interaction is still required to generate a complete fault interpretation. Today, image enhancement of fault-sensitive attributes, multiattribute fault analysis using shallow learning, and deep-learning algorithms based on extensive training and convolutional neural networks (CNNs) are promising fault interpretation workflows. We have compared three workflows to test fault-detection capabilities; these include image enhancement, probabilistic neural networks (PNNs), and CNNs. We compared results to human-interpreted faults as our ground truth for a merged 3D seismic survey acquired in the Taranaki Basin, New Zealand. We extracted fault surfaces from the results of the workflows using them as seed points for an active contour method. Extracted faults are then compared to the human-interpreted surface using the Hausdorff distance. Data conditioning, including spectral balancing and structure-oriented filtering, improved the performance of all three workflows. Although all three approaches produce enhanced fault volumes, we find differences in fault location and different artifacts (mispredicted faults). Because all three methods exhibit “false positive” predictions, the enhanced multispectral coherence method produces faults and stratigraphic edges in the final image, including residual stair-step artifacts. In our implementation, PNN produces many salt-and-pepper artifacts through the resulting image, suggesting that we might need to include better training data or reduce the volume size to reduce the number of relevant classes to obtain an improved classification. The CNN algorithm is trained with synthetic data that provide rapid results, correctly identifying larger faults, but missing smaller faults and, in some cases, misclassifying mass-transport deposits and angular unconformities as being faults.
Propagation of instability fronts in modulationally unstable systems
Authors: Kamchatnov A. M. , Shaykin D. V. 
Published: January 2022

Abstract
We study the evolution of pulses propagating through focusing nonlinear media. A small disturbance of a smooth initial non-uniform pulse amplitude leads to formation of a region of strong nonlinear oscillations. We develop here an asymptotic method for finding the law of motion of the front of this region. The method is based on the conjecture that instability fronts propagate with the minimal group velocity of linear waves. To support this conjecture, at first we review several physical situations where this statement was obtained as a result of direct calculations. Then we generalize it to situations with a non-uniform flow and apply it to the focusing nonlinear Schrödinger equation for the particular cases of Talanov and Akhmanov-Sukhorukov-Khokhlov initial distributions. The approximate analytical results agree very well with the exact numerical solutions for these two problems.
Latent TGFβ-binding proteins 1 and 3 protect the larval zebrafish outflow tract from aneurysmal dilatation
Authors: Abrial Maryline , Basu Sandeep , Huang Mengmeng , Butty Vincent , Schwertner Asya , Jeffrey Spencer , Jordan Daniel , Burns Caroline E. , Burns C. Geoffrey 
Published: January 2022

ABSTRACTAortic root aneurysm is a common cause of morbidity and mortality in Loeys-Dietz and Marfan syndromes, where perturbations in transforming growth factor beta (TGFβ) signaling play a causal or contributory role, respectively. Despite the advantages of cross-species disease modeling, animal models of aortic root aneurysm are largely restricted to genetically engineered mice. Here, we report that zebrafish devoid of the genes encoding latent-transforming growth factor beta-binding protein 1 and 3 (ltbp1 and ltbp3, respectively) develop rapid and severe aneurysm of the outflow tract (OFT), the aortic root equivalent. Similar to syndromic aneurysm tissue, the distended OFTs display evidence for paradoxical hyperactivated TGFβ signaling. RNA-sequencing revealed significant overlap between the molecular signatures of disease tissue from mutant zebrafish and a mouse model of Marfan syndrome. Moreover, chemical inhibition of TGFβ signaling in wild-type animals phenocopied mutants but chemical activation did not, demonstrating that TGFβ signaling is protective against aneurysm. Human relevance is supported by recent studies implicating genetic lesions in LTBP3 and, potentially, LTBP1 as heritable causes of aortic root aneurysm. Ultimately, our data demonstrate that zebrafish can now be leveraged to interrogate thoracic aneurysmal disease and identify novel lead compounds through small-molecule suppressor screens.This article has an associated First Person interview with the first author of the paper.
ALFY localizes to early endosomes and cellular protrusions to facilitate directional cell migration
Authors: Søreng Kristiane , Pankiv Serhiy , Bergsmark Camilla , Haugsten Ellen M. , Dahl Anette K. , de la Ballina Laura R. , Yamamoto Ai , Lystad Alf H. , Simonsen Anne 
Published: January 2022

ABSTRACT
Cell migration is a complex process underlying physiological and pathological processes such as brain development and cancer metastasis. The autophagy-linked FYVE protein (ALFY; also known as WDFY3), an autophagy adaptor protein known to promote clearance of protein aggregates, has been implicated in brain development and neural migration during cerebral cortical neurogenesis in mice. However, a specific role of ALFY in cell motility and extracellular matrix adhesion during migration has not been investigated. Here, we reveal a novel role for ALFY in the endocytic pathway and in cell migration. We show that ALFY localizes to RAB5- and EEA1-positive early endosomes in a PtdIns(3)P-dependent manner and is highly enriched in cellular protrusions at the leading and lagging edge of migrating cells. We find that cells lacking ALFY have reduced attachment and altered protein levels and glycosylation of integrins, resulting in the inability to form a proper leading edge and loss of directional cell motility.
Novel stress granule-like structures are induced via a paracrine mechanism during viral infection
Authors: Iadevaia Valentina , Burke James M. , Eke Lucy , Moller-Levet Carla , Parker Roy , Locker Nicolas 
Published: January 2022

ABSTRACTTo rapidly adapt to stresses such as infections, cells have evolved several mechanisms, which include the activation of stress response pathways and the innate immune response. These stress responses result in the rapid inhibition of translation and condensation of stalled mRNAs with RNA-binding proteins and signalling components into cytoplasmic biocondensates called stress granules (SGs). Increasing evidence suggests that SGs contribute to antiviral defence, and thus viruses need to evade these responses to propagate. We previously showed that feline calicivirus (FCV) impairs SG assembly by cleaving the scaffolding protein G3BP1. We also observed that uninfected bystander cells assembled G3BP1-positive granules, suggesting a paracrine response triggered by infection. We now present evidence that virus-free supernatant generated from infected cells can induce the formation of SG-like foci, which we name paracrine granules. They are linked to antiviral activity and exhibit specific kinetics of assembly-disassembly, and protein and RNA composition that are different from canonical SGs. We propose that this paracrine induction reflects a novel cellular defence mechanism to limit viral propagation and promote stress responses in bystander cells.
Selective microautophagy of proteasomes is initiated by ESCRT-0 and is promoted by proteasome ubiquitylation
Authors: Li Jianhui , Hochstrasser Mark 
Published: January 2022

ABSTRACT
The proteasome is central to proteolysis by the ubiquitin-proteasome system under normal growth conditions but is itself degraded through macroautophagy under nutrient stress. A recently described AMP-activated protein kinase (AMPK)-regulated endosomal sorting complex required for transport (ESCRT)-dependent microautophagy pathway also regulates proteasome trafficking and degradation in low-glucose conditions in yeast. Aberrant proteasomes are more prone to microautophagy, suggesting the ESCRT system fine-tunes proteasome quality control under low-glucose stress. Here, we uncover additional features of the selective microautophagy of proteasomes in budding yeast. Genetic or pharmacological induction of aberrant proteasomes is associated with increased mono- or oligo-ubiquitylation of proteasome components, which appears to be recognized by ESCRT-0. AMPK controls this pathway in part by regulating the trafficking of ESCRT-0 to the vacuole surface, which also leads to degradation of the Vps27 subunit of ESCRT-0. The Rsp5 ubiquitin ligase contributes to proteasome subunit ubiquitylation, and multiple ubiquitin-binding elements in Vps27 are involved in their recognition. We propose that ESCRT-0 at the vacuole surface recognizes ubiquitylated proteasomes and initiates their microautophagic elimination during glucose depletion.
This article has an associated First Person interview with the first author of the paper.
Metabolic trade-offs favor regulated hypothermia and inhibit fever in immune-challenged chicks
Authors: do Amaral-Silva Lara , da Silva Welex Cândido , Gargaglioni Luciane Helena , Bícego Kênia Cardoso 
Published: January 2022

ABSTRACT
The febrile response to resist a pathogen is energetically expensive, while regulated hypothermia seems to preserve energy for vital functions. We hypothesized here that immune-challenged birds facing metabolic trade-offs (reduced energy supply/increased energy demand) favor a regulated hypothermic response at the expense of fever. To test this hypothesis, we compared 5 day old broiler chicks exposed to fasting, cold (25°C), and fasting combined with cold with a control group fed under thermoneutral conditions (30°C). The chicks were injected with saline or with a high dose of endotoxin known to induce a biphasic thermal response composed of a drop in body temperature (Tb) followed by fever. Then Tb, oxygen consumption (metabolic rate), peripheral vasomotion (cutaneous heat exchange), breathing frequency (respiratory heat exchange) and huddling behavior (heat conservation indicator) were analyzed. Irrespective of metabolic trade-offs, chicks presented a transient regulated hypothermia in the first hour, which relied on a suppressed metabolic rate for all groups, increased breathing frequency for chicks fed/fasted at 30°C, and peripheral vasodilation in chicks fed/fasted at 25°C. Fever was observed only in chicks kept at thermoneutrality and was supported by peripheral vasoconstriction and huddling behavior. Fed and fasted chicks at 25°C completely eliminated fever despite the ability to increase metabolic rate for thermogenesis in the phase correspondent to fever when it was pharmacologically induced by 2,4-dinitrophenol. Our data suggest that increased competing demands affect chicks' response to an immune challenge, favoring regulated hypothermia to preserve energy while the high costs of fever to resist a pathogen are avoided.
Highly Active Ni/SiO2 Catalyst Prepared Through Citric Acid-Assisted Impregnation for the Hydrogenation of Acetoin to 2,3-Butanediol
Authors: Nakazono Kazuki , Hosaka Shuya , Yamada Yasuhiro , Sato Satoshi 
Published: January 2022

Abstract
The vapor-phase hydrogenation of acetoin (AC) to 2,3-butanediol (2,3-BDO) was investigated over SiO2-supported Ni (Ni/SiO2) catalysts prepared by impregnation assisted with organic additives. Among 14 catalysts with different organic additives, the Ni/SiO2 prepared with citric acid (CA) was found to show the highest activity. The Ni/SiO2 catalyst prepared with CA has a much higher hydrogenation ability for AC and a higher selectivity to 2,3-BDO than typical noble metal catalysts. In addition, it was preferable that the hydrogenation of AC was conducted at a low temperature in the hydrogenation-dehydrogenation equilibrium between AC and 2,3-BDO to provide 2,3-BDO selectively.
Assessment of nurses’ and assistants’ knowledge on the prevention and management of pressure ulcers
Authors: Vrachni Eleni , Kaba Evridiki , Fasoi Georgia , Stavropoulou Areti , Bourazani Maria , Kelesi Martha 
Published: January 2022

Background: The pressure ulcers constitute a major clinical problem with enormous socio-economic consequences worldwide. Nurses' knowledge on pressure ulcers’ management plays a crucial role in the prevalence of pressure ulcers.Aim: The investigation of nurses’ knowledge, working in a general hospital, regarding the prevention and management of pressure ulcers. Material and Method: It was a cross-sectional study using a structured questionnaire. The sample consisted of 54 nurses and assistants working in medical and surgical departments of a General Hospital in Athens, Greece. The collection of data took place from June 2019 until November 2019. The analysis of data conducted using the IBM SPSS® program, version 25 (IBM Corp. in Armonk, NY).Results: The mean age of the participants was 42,73 ±8,42 years, and 90,6% were women, with an average work experience of 19,05±9,84 years. The age of nurses of the sample was not associated with the knowledge regarding the prevention of pressure ulcers (p = 0,80). No significant differences were found between their knowledge and the department of work, or the years of work experience and the related seminars organized in the department (p > 0,05). Nurses who had a postgraduate degree had statistically significant more correct answers (p = 0,01).Conclusions: Despite the development of nursing science in the treatment of pressure ulcers, nurses continue to present insufficient knowledge in the daily clinical practice. Τhe implementation of clinical guidelines and the continuing education courses may help nurses to enhance their knowledge.
Temporal Risk: Utility vs. Probability Weighting
Authors: Abdellaoui Mohammed , Diecidue Enrico , Kemel Emmanuel , Onculer Ayse 
Published: January 2022

This paper reports two experiments in which attitudes toward temporal risk resolution is elicited from choices between two-outcome lotteries that pay out at some future fixed date and can be resolved either now or later. We show that matching probabilities provides a simple method to measure attitudes toward temporal resolution—via the utility scale—under the recursive expected utility of Kreps and Porteus. We also analyze our data using a general recursive model that can reveal attitudes toward temporal risk resolution through the utility scale and/or the probability weighting scale. In terms of goodness of fit, as well as of prediction accuracy, our results point to a better performance of the probability weighting approach. More specifically, we show that individuals become less sensitive and more pessimistic with respect to winning probabilities when lotteries are resolved later rather than now. This paper was accepted by George Wu, decision analysis.