bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic Profiling of Hypoplastic Lungs Suggests an Underlying Inflammatory Response in the Pathogenesis of Abnormal Lung Development in Congenital Diaphragmatic Hernia
Authors: Wagner Richard , Lieckfeldt Paula , Piyadasa Hadeesha , Markel Moritz , Riedel Jan , Stefanovici Camelia , Peukert Nicole , Patel Daywin , Derraugh Gabrielle , Min Suyin Lum , Gosemann Jan-Hendrik , Deprest Jan , Pascoe Christopher D. , Tse Andrew , Lacher Martin , Mookherjee Neeloffer , Keijzer Richard 
Published: March 2022

AbstractThe pathogenesis of lung hypoplasia in congenital diaphragmatic hernia (CDH), a common birth defect, is poorly understood. The diaphragmatic defect can be repaired surgically, but the abnormal lung development contributes to a high mortality in these patients. To better understand the underlying pathobiology, we used the nitrofen rat model of CDH and characterized the proteome of hypoplastic CDH lungs at the alveolar stage (E21). Amongst the 218 significantly altered proteins between CDH and control lungs were Tenascin C, CREBBP, LYN and STAT3. We showed that Tenascin C was decreased around the distal airway branches in nitrofen and human fetal CDH lungs. In contrast, STAT3 was significantly increased in the airway epithelium of nitrofen lungs at E21. STAT3 inhibition after direct nitrofen exposure to fetal rat lung explants (E14.5) partially reversed the hypoplastic lung phenotype ex vivo by increasing peripheral lung budding. Moreover, we demonstrated that several STAT3 associated cytokines (IL-15, IL-9, IL-2) are increased in fetal tracheal aspirates of CDH survivors compared to non-survivors after fetoscopic tracheal occlusion. Using pathway analysis for significantly altered proteins in our proteomic analysis, we observed an enrichment in inflammatory response associated with Epstein Barr Virus and cytokine signaling in nitrofen CDH lungs. However, we were unable to detect EBV mRNA via in-situ Hybridization in human CDH lungs. With our unbiased proteomics approach, we show for the first time that inflammatory processes are likely underlying the pathogenesis of abnormal lung development in CDH.
Human repair-related Schwann cells adopt functions of antigen-presenting cells in vitro
Authors: Berner Jakob , Weiss Tamara , Sorger Helena , Rifatbegovic Fikret , Kauer Max , Windhager Reinhard , Dohnal Alexander , Ambros Peter F. , Ambros Inge M. , Steinberger Peter , Taschner-Mandl Sabine 
Published: March 2022

AbstractThe plastic potential of Schwann cells (SCs) is increasingly recognized to play a role after nerve injury and in diseases of the peripheral nervous system. In addition, reports on the interaction between SCs and immune cells indicate their involvement in inflammatory processes. However, data about the immunocompetence of human SCs are primarily derived from neuropathies and it is currently unknown whether SCs directly regulate an adaptive immune response after nerve injury.Here, we performed a comprehensive analysis of the immunomodulatory capacities of human repair-related SCs (hrSCs), which recapitulate SC response to nerve injury in vitro. We used our previously established protocol for the culture of primary hrSCs from human peripheral nerves and analyzed the transcriptome, secretome, and cell surface proteins for signatures and markers relevant in innate and adaptive immunity, performed phagocytosis assays, and monitored T-cell subset activation in co-cultures with autologous human T-cells.Our findings show that hrSCs are highly phagocytic, which is in line with high MHCII expression. In addition, hrSCs express co-regulatory molecules, such as CD40, CD80, B7H3, CD58, CD86, HVEM, release a plethora of chemoattractants, matrix remodelling proteins and pro- as well as anti-inflammatory cytokines, and upregulate the T-cell inhibiting PD-L1 molecule upon pro-inflammatory stimulation with IFNγ. Furthermore, hrSC contact reduced the number and activation status of allogenic CD4+ and CD8+ T-cells.This study demonstrates that hrSCs possess features and functions typical for professional antigen presenting cells in vitro, and suggest a new role of these cells as negative regulators of T-cell immunity during nerve regeneration.Main pointsHuman repair-related Schwann cells (hrSC) function as professional antigen presenting cells.HrSCs up-regulate PD-L1 upon pro-inflammatory IFNγ stimulation.HrSCs hamper CD4+ and CD8+ T-cell activation.Graphical abstract
A comparison of six DNA extraction protocols for 16S, ITS, and shotgun metagenomic sequencing of microbial communities
Authors: Shaffer Justin P. , Carpenter Carolina S. , Martino Cameron , Salido Rodolfo A. , Minich Jeremiah J. , Bryant MacKenzie , Sanders Karenina , Schwartz Tara , Humphrey Gregory , Swafford Austin D. , Knight Rob 
Published: March 2022

AbstractMicrobial communities contain a broad phylogenetic diversity of organisms, however the majority of methods center on describing bacteria and archaea. Fungi are important symbionts in many ecosystems, and are potentially important members of the human microbiome, beyond those that can cause disease. To expand our analysis of microbial communities to include fungal ITS data, we compared five candidate DNA extraction kits against our standardized protocol for describing bacteria and archaea using 16S rRNA gene amplicon- and shotgun metagenomics sequencing. We present results considering a diverse panel of host-associated and environmental sample types, and comparing the cost, processing time, well-to-well contamination, DNA yield, limit of detection, and microbial community composition among protocols. Across all criteria, we found the MagMAX Microbiome kit to perform best. The PowerSoil Pro kit performed comparably, but with increased cost per sample and overall processing time. The Zymo MagBead, NucleoMag Food, and Norgen Stool kits were included.Accession numbersRaw sequence data were deposited at the European Nucleotide Archive (accession#: ERP124610) and raw and processed data are available at Qiita (Study ID: 12201). All processing and analysis code is available on GitHub (github.com/justinshaffer/Extraction_kit_testing).Methods summaryTo allow for downstream applications involving fungi in addition to bacteria and archaea, we compared five DNA extraction kits with our previously established, standardized protocol for extracting DNA for microbial community analysis. Across ten diverse sample types, we found one extraction kit to perform comparably or better than our standardized protocol. Our conclusion is based on per-sample comparisons of DNA yield, the number of quality-filtered sequences generated, the limit of detection of microbial cells, microbial community alpha-diversity, beta-diversity, and taxonomic composition, and extent of well-to-well contamination.
The MIF SNP rs755622 is a germline determinant of tumor immune activation in Glioblastoma
Authors: Alban Tyler J. , Grabowski Matthew M. , Otvos Balint , Bayik Defne , Zalavadia Ajay , Makarov Vlad , Troike Katie , McGraw Mary , Rabljenovic Anja , Lauko Adam , Neumann Chase , Roversi Gustavo , Waite Kristin A. , Cioffi Gino , Patil Nirav , Tran Thuy T. , McCortney Kathleen , Steffens Alicia , Diaz C. Marcela , Brown J. Mark , Egan Kathleen M. , Horbinski Craig M. , Barnholtz-Sloan Jill S. , Vogelbaum Michael A. , Bucala Richard , Chan Timothy A. , Ahluwalia Manmeet S. , Lathia Justin D. 
Published: March 2022

AbstractWhile immunotherapies have shown durable responses for multiple tumors, their efficacy remains limited in some advanced cancers, including glioblastoma. This may be due to differences in the immune landscape, as the glioblastoma microenvironment strongly favors immunosuppressive myeloid cells, which are linked to an elevation in immune-suppressive cytokines, including macrophage migration inhibitory factor (MIF). We now find that a single-nucleotide polymorphism (SNP) rs755622 in the MIF promoter associates with increased leukocyte infiltration in glioblastoma. Furthermore, we identified lactotransferrin expression as being associated with the rs755622 SNP, which could also be used as a biomarker for immune infiltrated tumors. These findings provide the first example in glioblastoma of a germline SNP that underlies differences in the immune microenvironment and identifies high lactotransferrin as a potential factor promoting immune activation.
Generalized Wintgen inequality for BI-SLANT submanifolds in conformal Sasakian space form with quarter-symmetric connection
Authors: Aslam Mohd , Siddiqi Mohd Danish , Siddiqui Aliya Naaz 
Published: March 2022

PurposeIn 1979, P. Wintgen obtained a basic relationship between the extrinsic normal curvature the intrinsic Gauss curvature, and squared mean curvature of any surface in a Euclidean 4-space with the equality holding if and only if the curvature ellipse is a circle. In 1999, P. J. De Smet, F. Dillen, L. Verstraelen and L. Vrancken gave a conjecture of Wintgen inequality, named as the DDVV-conjecture, for general Riemannian submanifolds in real space forms. Later on, this conjecture was proven to be true by Z. Lu and by Ge and Z. Tang independently. Since then, the study of Wintgen’s inequalities and Wintgen ideal submanifolds has attracted many researchers, and a lot of interesting results have been found during the last 15 years. The main purpose of this paper is to extend this conjecture of Wintgen inequality for bi-slant submanifold in conformal Sasakian space form endowed with a quarter symmetric metric connection.Design/methodology/approachThe authors used standard technique for obtaining generalized Wintgen inequality for bi-slant submanifold in conformal Sasakian space form endowed with a quarter symmetric metric connection.FindingsThe authors establish the generalized Wintgen inequality for bi-slant submanifold in conformal Sasakian space form endowed with a quarter symmetric metric connection, and also find conditions under which the equality holds. Some particular cases are also stated.Originality/valueThe research may be a challenge for new developments focused on new relationships in terms of various invariants, for different types of submanifolds in that ambient space with several connections.
Examining coaches’ feedback to preservice teacher candidates on a core practice
Authors: Cash Anne Henry , Dack Hilary , Leach William 
Published: March 2022

PurposeFor preservice teacher candidates (PSTs), receiving feedback on core practices is an important component in supporting the development of their practice. However, coaches are often underprepared to support PSTs on core practices, and feedback can be infrequent or low quality (Anderson and Stillman, 2013; Clarke et al., 2014). Understanding such variation in the content and process for providing feedback to PSTs is important in evaluating and improving feedback effectiveness for amplifying their learning.Design/methodology/approachThe authors studied feedback provided by coaches in response to a video of a sample PST’s lesson. The authors examined the extent to which coaches’ feedback targeted the core practice of eliciting student thinking and whether this was associated with their assigned PSTs’ instructional practices during student teaching. The authors also questioned whether this aspect of coach feedback could be changed in response to professional development.FindingsThe results provide preliminary evidence that coaches vary in the extent to which they focus feedback on a particular practice, even when directed to do so. Moreover, when coaches provide focused feedback on a core practice, the PSTs that they coach use the core practice during student teaching. Further, coaches’ feedback can be improved through professional development.Originality/valueThis study contributes to a limited evidence base examining the association between feedback and PSTs’ observed practice. It also establishes that coach feedback can be improved with professional development. The authors discuss these results in the context of documenting and improving teacher preparation.
Assessing the effects of cost, revenue and profit efficiency on bank performance: empirical evidence from Indian banking
Authors: Rakshit Bijoy 
Published: March 2022

Purpose
This paper aims to investigate the effects of cost, revenue and profit efficiency on bank profitability in an emerging economy such as India over the period 1997 to 2017. Additionally, this study examines the effect of efficiency on profitability across different ownership groups for a panel of 70 Indian commercial banks.

Design/methodology/approach
In the first stage, using stochastic frontier analysis, we estimate the efficiency scores of cost, revenue and profit over the examined period. In the second stage, this study uses the two-step system generalized-method of moments dynamic panel approach to investigate the impact of several efficiency measures on bank profitability.

Findings
Results estimated through and system generalized-method of moments indicate that a higher level of cost, revenue and efficiency significantly improves India's bank profitability. Regarding ownership groups, this study finds that the public sector banks are most cost-efficient compared to private and foreign banks. Other bank-specific, macroeconomic and institutional variables have played a significant role in determining bank profitability.

Practical implications
The findings of the study extend some important policy implications. In light of the rapid decline in bank profitability, banks should focus on increasing the efficiency of their operations. Improvement in profit, cost and revenue efficiency can ameliorate bank performance significantly. Profit efficiency that takes into account both cost and revenue efficiency should be maintained reasonably to prevent the declining pattern of bank profitability that the industry has witnessed over the years.

Originality/value
To the best of the author's knowledge, this study is a fresh piece of research that fulfils an urgent need of investigating the dynamics between bank efficiency and bank profitability in India. In an emerging economy like India, where the banking sector has witnessed substantial structural transformations over the past two decades, such study demands an immediate empirical investigation.
High-performance work systems and organizational citizenship behavior: the role of goal congruence and servant leadership
Authors: Arefin Md. Shamsul , Faroque Omar , Zhang Junwei , Long Lirong 
Published: March 2022

PurposeAligning employees' goals with organizational goals is an overarching objective of an organization to increase employees' outcomes and, ultimately, the firm's performance. Employees' perceived goal congruence is proposed to be an important mediator of the effect of high-performance work systems (HPWS) on organizational citizenship behaviors (OCB). In this paper, the authors proposed and tested a moderated mediation model that depicted how servant leadership increased or restrained these effects.Design/methodology/approachThis study used data from 56 managers and 322 employees working in Bangladeshi organizations. The study conducted cross-level analyses using hierarchical linear modeling (HLM) to examine the hypothetical relationships among variables.FindingsThis study revealed that employees' perceived goal congruence mediated the influence of HPWS on OCB. Consistent with the moderated mediation prediction, employee-perceived goal congruence mediated the relationship between HPWS and OCB when servant leadership is high.Originality/valueThis study examined how and when HPWS affects OCB by incorporating perceived goal congruence and servant leadership as mediating and moderating variables, respectively.
Modelling ragpickers’ productivity at the bottom of the pyramid: the use of artificial neural networks (ANNs)
Authors: Johnson Neil , Prasad Sameer , Vahedian Amin , Altay Nezih , Jain Ashish 
Published: March 2022

PurposeIn this research, the authors apply artificial neural networks (ANNs) to uncover non-linear relationships among factors that influence the productivity of ragpickers in the Indian context.Design/methodology/approachA broad long-term action research program provides a means to shape the research question and posit relevant factors, whereas ANNs capture the true underlying non-linear relationships. ANN models the relationships between four independent variables and three forms of waste value chains without assuming any distributional forms. The authors apply bootstrapping in conjunction with ANNs.FindingsThe authors identify four elements that influence ragpickers’ productivity: receptiveness to non-governmental organizations, literacy, the deployment of proper equipment/technology and group size.Research limitations/implicationsThis study provides a unique way to analyze bottom of the pyramid (BoP) operations via ANNs.Social implicationsThis study provides a road map to help ragpickers in India raise incomes while simultaneously improving recycling rates.Originality/valueThis research is grounded in the stakeholder resource-based view and the network–individual–resource model. It generalizes these theories to the informal waste value chain at BoP communities.
Quality of smart-work support service, perceived value and intention to continue smart-work: empirical evidence from Korea
Authors: Kim Sang Soo 
Published: March 2022

PurposeSmart-work has been attracting more attention since the COVID-19 outbreak hit the world in 2020. Smart-working practices do not always run smoothly despite the necessary infrastructure being in place. Taking the quality-value-loyalty chain and information system continuance model as the basis, this study aims to identify how “smart-work support service” leads to employees’ continuance intention toward smart-work. In this study, the smart-work support service refers to the infrastructure making smart-work possible and services needed to support smart-work.Design/methodology/approachUsing data collected from a survey of 406 employees working in Korea, this study verifies the components of smart-work support service quality and dimensions of perceived value through partial least squares analysis and then tests the proposed conceptual model using structural equation modeling.FindingsThe results verified that the quality of smart-work support service consists of eight attributes while dividing the perceived value of smart-work into three dimensions and further revealed that the service quality influences the intention to continue smart-working by way of perceived value and satisfaction.Originality/valueThis study sought to explore the relationship among service quality, perceived value and satisfaction by clearly conceptualizing and measuring them in the context of smart-work, thereby ultimately understanding the impact they have on the intention to continue smart-work.
From care packages to Zoom cookery classes: youth work during the COVID-19 “lockdown”
Authors: Shaw Aileen , Brady Bernadine , Dolan Patrick 
Published: March 2022

Purpose
This paper aims to explore the experience of one large Irish youth work organisation, Foróige, to measures introduced during the initial phase of COVID-19 in 2020. In the face of the unprecedented crisis including the closure of schools and curtailment of many youth services, this paper examines how the organisation responded and adapted its service offering.

Design/methodology/approach
Qualitative semi-structured interviews were conducted with 12 senior managers and youth officers in Foróige to explore their perspectives on the organisation’s response. Participants were purposively sampled from across the operational management functions and also from regional levels and youth workers engaging in work “on the ground”.

Findings
Shifting from a face-to -face, relationship-based to a distanced mode of engagement with young people, colleagues and volunteers required significant adaptation of Foróige’s service model. Innovation took place both in the delivery platform and fundamentally, in its service orientation. The accelerated move to online youth work brought about by the pandemic enabled the organisation to embrace and learn from the challenges and opportunities posed by digital technology. Responding to the immediate and tangible needs of young people in receipt of services, staff found themselves working with families at the more basic levels of intervention.

Originality/value
This paper provides new insights into the nature of non-profit service innovation during a time of unprecedented crisis management. It highlights characteristics of organisational agility that can assist organisations in managing crises, while also pointing the way towards a more flexible operating model for youth work service delivery.
Individuals responsible for video games: an exploration of cataloging practice, user need and authorship theory
Authors: Cho Hyerim , Hubbles Chris , Moulaison-Sandy Heather 
Published: March 2022

PurposeAuthor information is one of the primary metadata elements for information access. While assigning “author(s)” has been relatively straightforward in library systems for textual resources, challenges have emerged in recording creatorship information for collaborative creative works, with surrogates erring on the side of caution and providing little information. This study aims to present improvements to the conceptual understanding of collaborative creatorship and relevant cataloging practice in video games.Design/methodology/approachThe current study is a theoretical investigation of the authorship role of individuals in collaborative creative works, using video games as a case study. The investigation is based on the literature on video game user needs and authorship theory.FindingsReviews of literature present a disconnect between video game information user needs and currently available author information in library systems. Further analysis of the author/creator concept reveals insufficiencies in adopting auteur theory as the theory is applied to film. Exploration of access practices for other large collaborative creative products and an analysis of user tasks show potentially fruitful directions for future studies. This study recommends identifying primary roles that individuals adopt in video game creations and leveraging crowdsourced-creator information in library databases to enhance the visibility of author information for video games.Originality/valueBy incorporating authorship theories and research from various domains such as film studies, intellectual history and library and information science, this study provides interdisciplinary, theoretical considerations as well as practical suggestions to enhance the current cataloging practice.