bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transmission networks of hepatitis C virus among HIV/HCV-coinfected patients in Guangdong, China
Authors: Deng Xizi , Liang Zhiwei , Cai Weiping , Li Feng , Li Junbin , Hu Fengyu , Lan Yun 
Published: July 2022

Abstract
Background
Coinfection with hepatitis C virus (HCV) is common in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients due to shared routes of transmission. We aimed to investigate the characteristics of HCV subgenotypes among HIV/HCV-coinfected patients in Guangdong and explore the molecular transmission networks and related risk factors for HCV strains.

Methods
Plasma samples were obtained from 356 HIV/HCV-coinfected patients for HCV NS5B region sequencing. A neighbor-joining phylogenetic tree was constructed to affirm HCV subgenotypes. The transmission networks based on maximum likelihood phylogenetic tree were determined by Cluster Picker, and visualized using Cytoscape 3.2.1.

Results
A total of 302 HCV NS5B sequences were successfully amplified and sequenced from the 356 plasma samples. A neighbor-joining phylogenetic tree based on the 302 NS5B sequences revealed the profile of HCV subgenotypes circulating among HIV/HCV coinfection patients in Guangdong. Two predominant strains were found to be 6a (58.28%, 176/302) and 1b (18.54%, 56/302), followed by 3a (10.93%, 33/302), 3b (6.95%, 21/302), 1a (3.64%, 11/302), 2a (0.99%, 3/302) and 6n (0.66%, 2/302). A molecular transmission network of five major HCV genotypes was constructed, with a clustering rate of 44.04%. The clustering rates of subgenotypes 1a, 3a, 3b, 1b, and 6a were 18.18% (2/11), 42.42%, 52.38%, 48.21%, and 44.89%, respectively. Multivariate logistic regression analysis showed no significant effects from sex, age, transmission route, geographical region, baseline CD4 + T cell count or subgenotype (P > 0.05), except marital status. Married or cohabiting people (compared with unmarried people) had more difficulty forming transmission networks.

Conclusions
In summary, this study, based on HCV NS5B subgenotypes, revealed the HCV subtype diversity and distribution among HIV/HCV-coinfected patients in Guangdong. Marital status inclined to be the factor influencing HCV transmission networks formation.
Developing mental health services during and in the aftermath of the Ebola virus disease outbreak in armed conflict settings: a scoping review
Authors: Vivalya Bives Mutume Nzanzu , Vagheni Martial Mumbere , Kitoko Germain Manzekele Bin , Vutegha Jeremie Muhindo , Kalume Augustin Kensale , Piripiri Astride Lina , Masika Yvonne Duagani , Mbeva Jean-Bosco Kahindo 
Published: July 2022

Abstract
Background
Mental health is mostly affected by numerous socioeconomic factors that need to be addressed through comprehensive strategies. The aftermath of armed conflict and natural disasters such as Ebola disease virus (EVD) outbreaks is frequently associated with poor access to mental healthcare. To design the basis of improving mental health services via the integration of mental health into primary health care in the Democratic Republic of Congo (DRC), we conducted a scoping review of available literature regarding mental illness in armed conflict and EVD outbreak settings.

Methods
This scoping review of studies conducted in armed conflict and EVD outbreak of DRC settings synthesize the findings and suggestions related to improve the provision of mental health services. We sued the extension of Preferred Reporting Items for Systematic Reviews and Meta-Analyses to scoping studies. A mapping of evidence related to mental disorders in the eastern part of DRC from studies identified through searches of electronic databases (MEDLINE, Scopus, Psych Info, Google Scholar, and CINAHL). Screening and extraction of data were conducted by two reviewers independently.

Results
This review identified seven papers and described the findings in a narrative approach. It reveals that the burden of mental illness is consistent, although mental healthcare is not integrated into primary health care. Access to mental healthcare requires the involvement of affected communities in their problem-solving process. This review highlights the basis of the implementation of a comprehensive mental health care, through the application of mental health Gap Action Program (mhGAP) at community level. Lastly, it calls for further implementation research perspectives on the integration of mental healthcare into the health system of areas affecting by civil instability and natural disasters.

Conclusion
This paper acknowledges poor implementation of community mental health services into primary health care in regions affected by armed conflict and natural disasters. All relevant stakeholders involved in the provision of mental health services should need to rethink to implementation of mhGAP into the emergency response against outbreaks and natural disasters.
Sonographic features differentiating early-stage ovarian clear cell carcinoma from endometrioma with atypical features
Authors: Huang Kuan-Ju , Li Ying-Xuan , Wu Chin-Jui , Chang Wen-Chun , Wei Lin-Hung , Sheu Bor-Ching 
Published: July 2022

Abstract
Background
Ovarian clear cell carcinoma (OCCC) is the most common endometriosis-associated ovarian cancer. Ovarian endometriosis may present with atypical or malignant sonographic features and interfere with clinical judgment about whether definitive surgical intervention is required.

Objective
To compare the characteristics of endometrioma with atypical features and OCCC.

Methods
This study enrolled patients with pathologic diagnoses of either endometrioma or OCCC. For patients with endometrioma, only those with atypical features, defined as the presence of at least one of the following sonographic characteristics: cyst diameter of 10 ± 1 cm, multi-cystic lesions, any solid component or papillary structure, and blood flow of any degree, were included.

Results
Sixty-three patients had endometriomas with atypical features, while 57 patients had OCCC. Patients with endometriomas were younger (39.33 ± 7.04 years vs. 53.11 ± 9.28 years, P < 0.01), had smaller cysts (7.81 ± 2.81 cm vs. 12.68 ± 4.60 cm, P < 0.01), and had smaller solid components (0.93 ± 1.74 cm vs. 4.82 ± 3.53 cm, P < 0.01). In contrast, OCCCs were associated with loss of ground-glass echogenicity (6.3% vs 68.4%, P < 0.01). In multivariate analysis, advanced age (> 47.5 years), large cysts (> 11.55 cm), large solid components (size > 1.37 cm), and loss of ground-glass echogenicity were independent factors suggestive of malignancy.

Conclusion
Advanced age, larger cyst sizes, larger solid component sizes, and loss of ground-glass echogenicity are major factors differentiating endometriomas from malignancies. For women in menopausal transition who have finished childbearing who present with endometrioma with atypical features, removal of the adnexa intact could be considered.
External validation of a machine learning model to predict hemodynamic instability in intensive care unit
Authors: Dung-Hung Chiang , Cong Tian , Zeyu Jiang , Yu-Shan Ou-Yang , Yung-Yan Lin 
Published: July 2022

Abstract
Background
Early prediction model of hemodynamic instability has the potential to improve the critical care, whereas limited external validation on the generalizability. We aimed to independently validate the Hemodynamic Stability Index (HSI), a multi-parameter machine learning model, in predicting hemodynamic instability in Asian patients.

Method
Hemodynamic instability was marked by using inotropic, vasopressor, significant fluid therapy, and/or blood transfusions. This retrospective study included among 15,967 ICU patients who aged 20 years or older (not included 20 years) and stayed in ICU for more than 6 h admitted to Taipei Veteran General Hospital (TPEVGH) between January 1, 2010, and March 31, 2020, of whom hemodynamic instability occurred in 3053 patients (prevalence = 19%). These patients in unstable group received at least one intervention during their ICU stays, and the HSI score of both stable and unstable group was calculated in every hour before intervention. The model performance was assessed using the area under the receiver operating characteristic curve (AUROC) and was compared to single indicators like systolic blood pressure (SBP) and shock index. The hemodynamic instability alarm was set by selecting optimal threshold with high sensitivity, acceptable specificity, and lead time before intervention was calculated to indicate when patients were firstly identified as high risk of hemodynamic instability.

Results
The AUROC of HSI was 0.76 (95% CI, 0.75–0.77), which performed significantly better than shock Index (0.7; 95% CI, 0.69–0.71) and SBP (0.69; 95% CI, 0.68–0.70). By selecting 0.7 as a threshold, HSI predicted 72% of all 3053 patients who received hemodynamic interventions with 67% in specificity. Time-varying results also showed that HSI score significantly outperformed single indicators even up to 24 h before intervention. And 95% unstable patients can be identified more than 5 h in advance.

Conclusions
The HSI has acceptable discrimination but underestimates the risk of stable patients in predicting the onset of hemodynamic instability in an external cohort.
Effects of oil pollution on soil microbial diversity in the Loess hilly areas, China
Authors: Shi Lei , Liu Zhongzheng , Yang Liangyan , Fan Wangtao 
Published: July 2022

Abstract
Purpose
Data support and theoretical basis for bioremediation and treatment of petroleum-contaminated soils in the Loess hills of Yan’an, northern Shaanxi.

Methods
The evolutionary characteristics of soil microbial diversity and community structure under different levels of oil pollution were studied by field sampling, indoor simulation experiments, and analyzed through assays, using the mine soils from Yan’an, Shaanxi Province, as the research object.

Results
Compared with clean soil, the microbial species in contaminated soil were significantly reduced, the dominant flora changed, and the flora capable of degrading petroleum pollutants increased significantly. The soil microbial diversity and community structure differed, although not significantly, between different pollution levels, but significantly from clean soil. In the uncontaminated soil (CK), the dominant soil microbial genera were mainly Pantoea, Sphingomonas, Thiothrix, and Nocardioides. The abundance of Pseudomonas, Pedobacter, Massilia, Nocardioides, and Acinetobacter in the soil increased after oil contamination, while Thiothrix, Sphingomonas, and Gemmatimonas decreased significantly.

Conclusions
After the soil was contaminated with petroleum, the microbial species in the soil decreased significantly, the dominant genera in the soil changed, and the relative abundance of bacteria groups capable of degrading petroleum pollutants increased. The genera that can degrade petroleum pollutants in the petroleum-contaminated soil in the study area mainly include Pseudomonas, Acinetobacter, Pedobacter, Acinetobacter, and Nocardioides, which provide a scientific basis for exploring It provides a scientific basis for exploring remediation methods suitable for petroleum-contaminated soil in this region.
Stability is essential for insecticidal activity of Vip3Aa toxin against Spodoptera exigua
Authors: Fu Bai-Wen , Xu Lian , Zheng Mei-Xia , Chen Qing-Xi , Shi Yan , Zhu Yu-Jing 
Published: July 2022

AbstractVegetative insecticidal proteins 3A (Vip3A) were important insecticidal proteins for control of lepidopteran pests. Previous study demonstrated that Vip3Aa and Vip3Ad showed significant difference in insecticidal activities against Spodoptera exigua, while the molecular mechanism remained ambiguous. Here we demonstrated that the difference in insecticidal activities between Vip3Aa and Vip3Ad might be caused by the difference in stability of Vip3Aa and Vip3Ad in S. exigua midgut protease. Vip3Aa was quite stable while Vip3Ad could be further degraded. Molecular dynamics simulation revealed that Vip3Aa was more stable than Vip3Ad, with smaller RMSD and RMSF value. Amino acid sequence alignment indicated that three were three extra prolines (P591, P605 and P779) located on Vip3Aa. We further identified that residue P591 played a crucial role on stability and insecticidal activity of Vip3Aa. Taken together, our study demonstrated that the stability was essential for the insecticidal activity of Vip3A toxins, which might provide new insight into the action mode of Vip3A toxins and contribute to the design Vip3A variants with improved stability and insecticidal activity.
The drivers and intensity of adoption of beekeeping in northwest Ethiopia
Authors: Andaregie Adino , Worku Aemro , Worku Asnake , Atinkut Lingerew , Astatkie Tessema 
Published: July 2022

Abstract
Background
Beekeeping activity is carried out in most parts of Ethiopia. However, despite the favorable agro-ecology for beekeeping practices and the high number of bee colonies the country is endowed with, the level of beekeeping adoption is low.

Methods
This study was conducted to identify determinants of the decision to adopt beekeeping, and the intensity of adoption by using a cross-sectional data collected from 772 rural households in Northwest Ethiopia. Stratified random sampling method was used to select the households, and the data were collected using a questionnaire. To achieve the objectives, Heckman two-stage sample selection model was used.

Results
The result of the first step Heckman model revealed that age and educational level of the household head, household size, extension visits, training, incentive, home consumption of honey, major economic activities of the household, perception towards better hives, distance to the nearest marketplace, the number of years the household stayed in the village, and location were the significant variables influencing rural households’ beekeeping adoption decision. The second step Heckman model revealed that livestock holding of a household head, number of extension visits, credit use, presence of honey bee pests, whether a household is engaged in swarm catching practices, and major economic activities of a household head were the variables that influence the intensity of beekeeping adoption significantly.

Conclusions
The findings of the study can be used to make evidence-based policy interventions to improve beekeeping adoption and the intensity of beekeeping adoption by rural households, which could also help to improve their livelihoods.
How humans adapt to hot climates learned from the recent research on tropical indigenes
Authors: Tochihara Yutaka , Wakabayashi Hitoshi , Lee Joo-Young , Wijayanto Titis , Hashiguchi Nobuko , Saat Mohamed 
Published: July 2022

AbstractThis review mainly aimed to introduce the findings of research projects comparing the responses of tropical and temperate indigenes to heat. From a questionnaire survey on thermal sensation and comfort of Indonesians and Japanese, we found that the thermal descriptor “cool” in tropical indigenes connotes a thermally comfortable feeling, suggesting that linguistic heat acclimatization exists on a cognitive level. Ten male students born and raised in Malaysia were invited to Fukuoka, Japan, and compared their responses with 10 Japanese male students with matched physical fitness and morphological characteristics. Cutaneous thermal sensitivity: The sensitivities were measured at 28 °C. The forehead warm sensitivity was significantly blunted in Malaysians. The less sensitivity to the warmth of tropical indigenes is advantageous in respect to withstanding heat stress with less discomfort and a greater ability to work in hot climates. Passive heat stress: Thermoregulatory responses, especially sweating, were investigated, during the lower leg hot bathing (42 °C for 60 min). The rectal temperature at rest was higher in Malaysians and increased smaller during immersion. There was no significant difference in the total amount of sweating between the two groups, while the local sweating on the forehead and thighs was lesser in Malaysians, suggesting distribution of sweating was different from Japanese. Exercise: Malaysian showed a significantly smaller increase in their rectal temperature during 55% maximal exercise for 60 min in heat (32 °C 70% relative humidity), even with a similar sweating and skin blood flow response in Japanese. The better heat tolerance in Malaysians could be explained by the greater convective heat transfer from the body core to the skin due to the greater core-to-skin temperature gradient. In addition, when they were hydrated, Malaysian participants showed better body fluid regulation with smaller reduction in plasma volume at the end of the exercise compared to the non-hydrated condition, whereas Japanese showed no difference between hydration conditions. We further investigated the de-acclimatization of heat adaptation by longitudinal observation on the heat tolerance of international students who had moved from tropical areas to Fukuoka for several years.
Consequential research of accountability testing: the case of the CET
Authors: Jin Yan 
Published: July 2022

AbstractThis article examines the changes taken place over the decades after a test-based accountability system was in place and proposes an agenda for consequential research of the testing program. The article starts with a review of theories of test-based accountability (Supovitz, Journal of Educational Change 10:211–227, 2009) and a socially grounded, integrated approach to test validation (Chalhoub-Deville, Language Testing 33:453–472, 2016; Chalhoub-Deville and O’Sullivan, Validity: Theoretical development and integrated arguments, 2020). Following Supovitz’s frameworks of accountability testing, the case of the College English Test (CET), a national English language testing program in China, is analyzed to explain the nature of the consequences of accountability testing and show the motivational effects, alignment effects, the informational role and the symbolic values of the accountability testing program. The case analysis highlights the importance of investigating consequences of an accountability test at the aggregate and the system levels. Guided by Chalhoub-Deville and O’Sullivan’s (Validity: Theoretical development and integrated arguments, 2020) integrated validity framework, the article delineates the claims for the theory of action argument and the communication engagement argument for future investigation of the consequences of the CET.
‘Everything is data’: towards one big data ecosystem using multiple sources of data on higher education in Indonesia
Authors: Yunita Ariana , Santoso Harry B. , Hasibuan Zainal A. 
Published: July 2022

AbstractBig data is increasingly being promoted as a game changer for the future of science, as the volume of data has exploded in recent years. Big data characterized, among others, the data comes from multiple sources, multi-format, comply to 5-V’s in nature (value, volume, velocity, variety, and veracity). Big data also constitutes structured data, semi-structured data, and unstructured-data. These characteristics of big data formed “big data ecosystem” that have various active nodes involved. Regardless such complex characteristics of big data, the studies show that there exists inherent structure that can be very useful to provide meaningful solutions for various problems. One of the problems is anticipating proper action to students’ achievement. It is common practice that lecturer treat his/her class with “one-size-fits-all” policy and strategy. Whilst, the degree of students’ understanding, due to several factors, may not the same. Furthermore, it is often too late to take action to rescue the student’s achievement in trouble. This study attempted to gather all possible features involved from multiple data sources: national education databases, reports, webpages and so forth. The multiple data sources comprise data on undergraduate students from 13 provinces in Indonesia, including students’ academic histories, demographic profiles and socioeconomic backgrounds and institutional information (i.e. level of accreditation, programmes of study, type of university, geographical location). Gathered data is furthermore preprocessed using various techniques to overcome missing value, data categorisation, data consistency, data quality assurance, to produce relatively clean and sound big dataset. Principal component analysis (PCA) is employed in order to reduce dimensions of big dataset and furthermore use K-Means methods to reveal clusters (inherent structure) that may occur in that big dataset. There are 7 clusters suggested by K-Means analysis: 1. very low-risk students, 2. low-risk students, 3. moderate-risk students, 4. fluctuating-risk students, 5. high risk students, 6. very high-risk students and, 7. fail students. Among the clusters unreveal, (1) a gap between public universities and private universities across the three regions in Indonesia, (2) a gap between STEM and non-STEM programmes of study, (3) a gap between rural versus urban, (4) a gap of accreditation status, (5) a gap of quality human resources distribution, etc. Further study, we will use the characteristics of each cluster to predict students’ achievement based on students’ profiles, and provide solutions and interventions strategies for students to improve their likely success.
Exploring the appropriate test methods to examine the effects of firefighter personal protective equipment on mobility
Authors: Son Su-Young , Muraki Satoshi , Tochihara Yutaka 
Published: July 2022

AbstractThis study aimed to ascertain the appropriate test methods for the investigation of the effect of personal protective equipment on mobility of firefighters. Nine male volunteers performed a physical performance test (crawl, obstacle course, side-to-side jumps, heavy-object drag, and step-ups), functional balance test (functional reach, timed up and go, and wooden plank time), range of motion test, and subjective evaluation under four clothing conditions with different weights and combinations of personal protective equipment. The results showed that physical performance, functional balance ability, and joint angles significantly declined with increased weight of personal protective equipment. On subjective evaluation, wearing heavy personal protective equipment increased perceived exertion and discomfort on movement. Our results proved that some of our test methods, including the obstacle course, side-to-side jumps, functional reach, timed up and go, range of motion test, and subjective evaluation, can be used to examine the mobility of personal protective equipment wearers. The findings of this study provide an effective guide for researchers and firefighters regarding the evaluation of mobility with personal protective equipment, as well as for manufacturers in the development of enhanced, comfortable personal protective equipment.