bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recovery of male reproductive endocrine function after ceasing prolonged testosterone undecanoate injections
Authors: Handelsman David J , Desai Reena , Conway Ann J , Shankara-Narayana Nandini , Stuckey Bronwyn G A , Inder Warrick J , Grossmann Mathis , Yeap Bu Beng , Jesudason David , Ly Lam P , Bracken Karen , Wittert Gary Allen 
Published: January 2022

Context
The time course of male reproductive hormone recovery after stopping injectable testosterone undecanoate (TU) treatment is not known.

Objective
The aim of this study was to investigate the rate, extent, and determinants of reproductive hormone recovery over 12 months after stopping TU injections.

Materials and Methods
Men (n = 303) with glucose intolerance but without pathologic hypogonadism who completed a 2-year placebo (P)-controlled randomized clinical trial of TU treatment were recruited for further 12 months while remaining blinded to treatment. Sex steroids (testosterone (T), dihydrotestosterone, oestradiol, oestrone) by liquid chromatography-mass sprectometry, luteinizing hormone (LH), follicle-stimulating hormone (FSH) and sex hormone-binding globulin (SHBG) by immunoassays and sexual function questionnaires (Psychosexual Diary Questionnaire, International Index of Erectile Function, and short form survey (SF-12)) were measured at entry (3 months after the last injection) and 6, 12, 18, 24, 40, and 52 weeks later.

Results
In the nested cohort of TU-treated men, serum T was initially higher but declined at 12 weeks remaining stable thereafter with serum T and SHBG at 11 and 13%, respectively, lower than P-treated men. Similarly, both questionnaires showed initial carry-over higher scores in T-treated men but after 18 weeks showed no difference between T- and P-treated men. Initially, fully suppressed serum LH and FSH recovered slowly towards the participant’s own pre-treatment baseline over 12 months since the last injection.

Conclusions
After stopping 2 years of 1000 mg injectable TU treatment, full reproductive hormone recovery is slow and progressive over 15 months since the last testosterone injection but may take longer than 12 months to be complete. Persistent proportionate reduction in serum SHBG and T reflects lasting exogenous T effects on hepatic SHBG secretion rather than androgen deficiency.
Challenges for ethics committees in biomedical research governance: Illustrations from China and Australia
Authors: Huanhuan Cao , Li Ming , Wang Mingxu , Roder David , Olver Ian 
Published: January 2022

In this paper, the evolution of the ethics committees for health research, their history, membership, and function in China and Australia is described. Investigators in each country compared the history and governance of their ethical systems based on the published evidence rather than personal opinions. Similarly, examples of challenges were selected from the literature. In both countries, the aim was to maximize the social benefits of research and minimize the risk imposed on the participants. Common challenges include maintaining independence, funding and delivering timely ethical reviews of the research projects. These challenges can be difficult where research ethics committees rely on voluntary contributions and lack a strong resource base. They must adapt to the increasingly rapid pace of research as well as the technological sophistication. Population health research can challenge the conventional views of consent and privacy. The principles of the sound ethical review are common in both countries; governance arrangements and operational procedures, however, can differ, reflecting the cultural values and norms of their host countries and in respect of legal environments. By studying the evolution and function of ethics committees in the two countries, we established the differences in the governance and health systems, while similar ethical objectives helped sustain collaborative research.
In Vitro Antibacterial Activity, Preliminary Phytochemical Screening Profile, and In Vivo Toxicity of Seven Traditional Medicinal Plants in Ethiopia
Authors: Mekuriaw Eyerus , Mengistu Enat , Erdedo Ayana , Mamo Hassen 
Published: January 2022

The threat of antibiotic-resistance calls for novel antibacterial agents. This study was aimed at screening medicinal plants for their antibacterial properties, phytochemical content and safety. Leaves of Allophylus abyssinicus (Hochst.) Radlk., Dicliptera laxata C.B.Clarke, Ligustrum vulgare L., Solanecio gigas (Vatke) c. Jeffrey and Gymnanthemum myrianthum (Hook.f.) H.Rob.; leaf and stem-bark of Olinia rochetiana A. Juss. and the seed of Cucurbita pepo L. were used. Chloroform and ethanol were used to extract G. myrianthum, D. laxata and O. rochetiana; ethyl acetate and methanol for the rest, and water for all. The extracts were tested against clinical/standard strains of Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Staphylococcus aureus by the agar-diffusion method. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined. Acute toxicity to mice was checked and preliminary phytochemical screening was done. Thirteen extracts, out of 24, were active (inhibition zone >7 mm) at differing levels (9.67±0.33-25.66±0.57 mm) against at least one bacterial strain. The MICs and MBCs were 1.95-15.6 mg/mL and 7.8-125 mg/mL respectively. The aqueous extract of S. gigas, methanol extracts of L. vulgare and A. abyssinicus, and ethanol extract of O. rochetiana leaf were the most active (MIC 1.95mg/ml) against S. aureus. Ethyl acetate extracts of A. abyssinicus, L. vulgare and S. gigas; aqueous of C. pepo, O. rochetiana and G. myrianthum; and all D. laxata had no antibacterial activity. P. aeruginosa was the least susceptible to any extract, although the methanol and aqueous extracts of S. gigas performed better against it. Preliminary phytochemical screening of selected extracts for phenols, flavonoids, tannins, steroids, terpenoids, steroidal glycosides, alkaloids, saponins, resins and glycosides showed positivity at least for four of these phytochemicals with glycoside and terpenoids in nearly all extracts and resin in none. The plants were not toxic to mice at 2000 mg/kg. Further consideration of S. gigas, L. vulgare, A. abyssinicus and O. rochetiana is recommended in light of their promising potential and safety.
Effect of Osmo Dehydration on Quality Attributes of Bilimbi (Averroha bilimbi) Fruits
Authors: Aparna G.S. , Lekshmi P.R. Geetha , Mini C. 
Published: January 2022

Background: Bilimbi is a profusely bearing tree and majority of fruits produced are wasted due to lack of proper preservation methods. Osmo-dehydration studies on quality attributes of bilimbi (Averroha bilimbi) was conducted with the objective to standardize the process variables for osmodehydrated bilimbi and to assess the retention of bioactive compounds. Methods: Harvested mature bilimbi fruits of uniform size were washed, surface dried, pricked and blanched in hot water for one minute. Blanched fruits were subjected to osmotic treatment, with sucrose solution of 40, 60 and 80°B for 60, 120 and 180 minutes. The osmodehydrated bilimbi fruits were analyzed for mass transfer, biochemical and sensory qualities. Best treatments were stored for four months in the room temperature. Result: Mass transfer characters viz., solid gain, water loss, percentage weight reduction, yield and biochemical parameters such as reducing sugar and total sugar increased with increase in osmotic concentration and immersion time whereas free acids, ascorbic acid and antioxidant activity were decreased. The osmotic treatment of 80°B for 180 minutes recorded the highest value for solid gain (5.10%), water loss (16.72%), weight reduction (22.57%), ratio of water loss to solid gain (3.25%) and yield (21.13%) which exhibited superior sensory scores for taste (8.43), flavor (8.27), texture (8.46) and overall acceptability (8.43). The best three treatments selected based on sensory analysis were subjected to storage stability studies under room temperature. Osmodehydrated bilimbi obtained highest sensory score at the end of storage.
Mastitis Detection in Holstein Sahiwal Crossbred Cattle (Bos taurus) Using Different Brands and Dilution Levels of Liquid Anionic Surfactants
Authors: Waminal Yancy Ogdamin , Gambol Marjorie Raboy , Sabado Ahron Caiman , Vallejos John Paul Muring , Briones Ronaldo Curameng , Estabillo Esteward Jones Manlangit 
Published: January 2022

The study was conducted to evaluate the effect, consistency, and economic viability of using different brands and dilution levels of liquid anionic surfactants to detect Mastitis in dairy cattle. Fifty-five milk samples were collected and subjected to macroscopic evaluation at a cattle farm located in Sta. Maria, Bulacan, Philippines. Milk samples were brought to the Philippine Carabao Center National Headquarters and Genepool Science City of Muñoz, Nueva Ecija for Somatic Cell Count. The experiment used 10 treatments with 3 replications arranged in a Completely Randomized Design. The macroscopic evaluation results showed that liquid anionic surfactants at 30%-50% dilution levels could detect Mastitis in dairy cattle. This was validated by the somatic cell count results from PCC Dairy Laboratory. On the other hand, statistical analysis results revealed that the treatments and the control (commercial mastitis test) were comparable except for brand Z at a 40% dilution rate. Brand Z at 30% and 50% dilution rate were found to be consistent among other treatments in detecting Mastitis in dairy cattle. In terms of economic viability, brand X and brand Y at 30% were the cheapest and had the lowest production cost and highest percentage savings.
The study was conducted to evaluate the effect, consistency, and economic viability of using different brands and dilution levels of liquid anionic surfactants to detect Mastitis in dairy cattle. Fifty-five milk samples were collected and subjected to macroscopic evaluation at a cattle farm located in Sta. Maria, Bulacan, Philippines. Milk samples were brought to the Philippine Carabao Center National Headquarters and Genepool Science City of Muñoz, Nueva Ecija for Somatic Cell Count. The experiment used 10 treatments with 3 replications arranged in a Completely Randomized Design. The macroscopic evaluation results showed that liquid anionic surfactants at 30%-50% dilution levels could detect Mastitis in dairy cattle. This was validated by the somatic cell count results from PCC Dairy Laboratory. On the other hand, statistical analysis results revealed that the treatments and the control (commercial mastitis test) were comparable except for brand Z at a 40% dilution rate. Brand Z at 30% and 50% dilution rate were found to be consistent among other treatments in detecting Mastitis in dairy cattle. In terms of economic viability, brand X and brand Y at 30% were the cheapest and had the lowest production cost and highest percentage savings.
Supply and Value Chain Models in Cattle Marketing and Its Derivative Products in East Nusa Tenggara Province
Authors: R Lole Ulrikus , Keban Arnoldus , Sogen Johanes G. , Mulyantini Ni Gusti Ayu 
Published: January 2022

In general, the determination of the price of cattle is based on the condition of the cattle's body. This will result in an unsatisfactory transfer of revenue value for farmers, because the bargaining position is still weak. In addition, transactions in the marketing chain of beef products and their by-products have not yet been solidly established, so the obligations and rights of some parties are not guaranteed. The objective was to analyze the role of stakeholders in each supply chain and distribution of value chains as revenue in the marketing transactions of cattle, beef, and derivative products. The study was conducted in West Timor, which has 85.0% of the cattle population in NTT. Four sample districts (Kupang, TTS, TTU and Belu) in 8 sample sub-districts or 16 sample villages were included in the study. Respondents in marketing activities consisted of farmers, village traders, sub-district/district traders, slaughter traders, inter-island traders, by-product/waste traders, as well as beef, cowhide, bone, and fat/blood processing industries. Data collection were conducted by a questionnaire-based interview. The data were analyzed descriptively-quantitatively with a supply chain analysis model. It canbe concluded that (1) Market supply chains include cattle traders (farmers, village traders, sub-district traders, inter-island traders and slaughtering traders), as well as beef traders (fresh beef retailers, frozen beef exporting traders, and processed beef products traders); (2) The ideal model of the value chain in the form of revenue share from marketing of cattle, beef, processed beef products, by-products, and cattle waste has not been fully established in NTT; (3) The ideal model includes five marketing blocks (cattle block, beef block, processed beef block, by-product block, and waste block).
PENGEMBANGAN BROWNIES KUKUS TEPUNG TALAS-KECAMBAH KACANG HIJAU DAN UBI UNGU KUKUS (TALAHIBU) UNTUK IBU HAMIL KURANG ENERGI KRONIK (KEK)
Authors: Amalia Islamiati Putri , Winarsi Hery , Ramadhan Gumintang Ratna 
Published: January 2022

Background: The prevalence of pregnant women with a risk of Chronic Energy Deficiency (CED) is quite high (24.2%). CED in pregnant women requires additional feeding interventions (PMT). Brownies enriched with taro flour–mung bean sprouts and purple sweet potato (TALAHIBU) were chosen as it is an energy-dense product and expected to have preferred organoleptic value and energy as PMT.
Objectives: To determine the phenolic antioxidant content of the best TALAHIBU formula and to determine the serving size of TALAHIBU.
Methodology: This factorial experimental study used a randomized block design (RBD) with 2 factors, consisting of taro flour-mung bean sprout proportion and the percentage of added fine steamed sweet potato. Organoleptic variable analysis used Friedman test continued with 5% Multiple Appeal Test. The best treatment combination was chosen, continued with Proximate and phenolic test then energy calculations.
Results: The best treatment was TALAHIBU G2J2 with water content of 23.94% BW; ash content 3.16% bb; fat 25.7% BW; carbohydrates 45.57% bb; protein 1.63% bb; energy 419.83kcal; and phenolic antioxidant 19,1 mg/g.
Conclusion: The best product TALAHIBU is suitable PMT for pregnant women with CED by serving 5 slice brownies per day.
Diabetic Foot Ulcers and Risk Fall in People with Type 2 Diabetes Mellitus in BLUD RSUD Dr. Ben Mboi Manggarai Regency, NTT
Authors: Ningsih Oliva Suyen , Iwa Kornelia Romana , Simon Maria Getrida , Sinar Kataria Anastasia 
Published: January 2022

The prevalence of diabetes mellitus in Manggarai Regency in 2019 is 535 people and some of them have diabetic foot ulcers. Patients with diabetic foot ulcers had a higher risk of falling than others.This study was to determine the risk factors for diabetic foot ulcers and the risk of falls in patients with type 2 diabetes mellitus. A quantitative study using a cross-sectional study was conducted at BLUD RSUD dr. Ben Mboi in April-June 2020 (n = 51).The sampling technique used was purposive sampling with a questionnaire of diabetic foot screening and risk stratification form and Morse scale. Logistic regression results showed that there was a significant relationship between claudication (AOR: 8.409, 95% CI 1.664-42.500, p value 0.010), history of previous diabetic foot ulcers (AOR: 5,680, 95% CI 1,151-28,035, p value 0.033) with diabetic foot ulcers. There was a significant relationship between hypertension (AOR: 0.152, 95% CI 0.028-0.834, p value 0.030), diabetic foot ulcers (AOR: 11.392, 95% CI 1.277-101.651, p value 0.029) with the risk of falling for patients with type 2 diabetes mellitus. Assessment of the risk of diabetic foot ulcers and the risk of falls should be done in patients with diabetes mellitus with or without neuropathy peripheral
An Integrated Approach of Numerical Well Test for Well Intersecting Fractures Based on FMI Image
Authors: Jin Guodong , Xing Huilin , Li Tianbin , Zhang Rongxin , Liu Junbiao , Guo Zhiwei , Ma Zihan 
Published: January 2022

Abstract
Fluid flow is strongly affected by fractures in unconventional reservoirs. It is essential to deeply understand the flow characteristics with fractures for improving the production and efficiency of unconventional reservoir exploitation. The purpose of this work is to develop an accurate numerical model to evaluate the transient-pressure response for well intersecting fractures. The meshes generated from Fullbore Formation Micro-Imager (FMI) images ensure an efficient numerical description of the geometries for fractures and interlayers. The numerical simulation is implemented by an inhouse finite element method-based code and benchmarked with drill stem test (DST) data. The results show that three flow regimes appear in the reservoir with fractures within the test period: wellbore afterflow, pseudolinear flow, and radial flow. In contrast, only the wellbore afterflow and radial flow appear for the wells without fractures. The results also reveal that fractures dominate the flow near the wellbore. Verification and application of the model show the practicability of the integrated approach for investigating the transient-pressure behaviors in the unconventional reservoir.
Reservoir Architecture Modeling at Sub-Seismic Scale for a Depleted Carbonate Reef Reservoir for CO2 Storage in Sarawak Basin, Offshore Malaysia
Authors: Cai Zhong , Widyanita Ana , Chidambaram Prasanna , Jones Ernest A 
Published: January 2022

Abstract
It is still a challenge to build a numerical static reservoir model, based on limited data, to characterize reservoir architecture that corresponds to the geological concept models. The numerical static reef reservoir model has been evolving from the oversimplified tank-like models, simple multi-layer models to the complex multi-layer models that are more realistic representations of complex reservoirs. A simple multi-layer model for the reef reservoir with proportional layering scheme was applied in the CO2 Storage Development Plan (SDP) study, as the most-likely scenario to match the geological complexity. Model refinement can be conducted during CO2 injection phase with Measurement, Monitoring and Verification (MMV) technologies for CO2 plume distribution tracking.
The selected reservoir is a Middle to Late Miocene carbonate reef complex, with three phases of reef growth: 1) basal transgressive phase, 2) lower buildup phase, and 3) upper buildup phase. Three chronostratigraphic surfaces were identified on 3D seismic reflection data as the zone boundaries, which were then divided into sub-zones and layers. Four layering methods were compared, which are ‘proportional’, ’follow top’, ‘follow base’ and ‘follow top with reference surface’. The proportional layering method was selected for the base case of the 3D static reservoir model and the others were used in the uncertainty analysis. Based on the results of uncertainty and risk assessment, a risk mitigation for CO2 injection operation were modeled and three CO2 injection well locations were optimized. The reservoir architecture model would be updated and refined by the difference between the modeled CO2 plume patterns and The MMV results in the future.
First Successful Application of Multi-Stage Proppant Fracturing on Horizontal Well in Carbonate Reservoirs in Iraq
Authors: Zhu Dawei , Cui Mingyue , Chen Yandong , Wang Yongli , Ding Yunhong , Xiong Chunming , Liang Chong , Yao Fei , Wang Xiaoyong , Cai Wenxin , He Yanhui , Ling Zongfa , Wang Dayong 
Published: January 2022

Abstract
The carbonate reservoir S is a giant limestone reservoir in H Oilfield, Iraq. Although the reserves account for 25%, the production contribution is only 0.4% to the total oilfield production due to poor petrophysical properties. Accordingly, the first proppant fracturing on vertical well was successfully executed in December 2016, which has already achieved a steady production period over than 3 years. In order to further improve the productivity, the first multi-stage proppant fracturing(MSPF) on horizontal well(SH01X) was successfully applied in November 2019, a technique which is rarely reported for porous limestone reservoir in the Middle East.
Proppant fracturing in carbonate reservoirs is a technique difficulty worldwide, especially this is a lack of experiences in the Middle East. To ensure the success of this campaign, a holistic technical study including geology evaluation, reservoir performance analysis, drilling trajectory design, completion and fracturing technique design have been carried out based on principle of "geology-engineering integration". This paper will present a comprehensive illustration including treatment design (main completion-fracturing technique, total scale, fracturing fluid, proppant), job execution (mini-frac, main-frac) and post-frac production performance for this successful campaign.
True vertical depth (TVD) of Well SH01X is 2720 m and the horizontal section length is 811 m. Based on the main technique of multi-stage proppant fracturing with open hole packers and sliding sleeves, totally 3784.3 m3 fracturing fluid and 452 m3 proppant were pumped in 8 stages. The test production was 3214 BOPD (choke size: 40/64", wellhead pressure: 970 psi). A historical breakthrough in the productivity of S reservoir has been achieved by the campaign. The post-frac evaluation shows that the treatment parameters are consistent with the design. The connectivity between artificial fractures and formation is greatly improved, and the stimulation effect is significant. Currently the "production under controlled pressure" mode has been executed and the stable production under stimulation target rate has been maintained. The systematic "geology-engineering integration" workflow is of significance to the success of the treatment as well as the stimulation effect.
MSPF is planned to be a game-changing technique to develop the huge reserves of S reservoir. The experience gained from this case could provide theoretical as well as practical references for similar reservoirs in the Middle East.
Multiplexed and flexible neural coding in sensory, parietal, and frontal cortices during goal-directed virtual navigation
Authors: Noel Jean-Paul , Balzani Edoardo , Avila Eric , Lakshminarasimhan Kaushik , Bruni Stefania , Alefantis Panos , Savin Cristina , Angelaki Dora 
Published: January 2022

Abstract
We do not understand how neural nodes operate within the recurrent action-perception loops that characterize naturalistic self-environment interactions, nor how brain networks reconfigure during changing computational demands. Here, we record local field potentials (LFPs) and spiking activity simultaneously from the dorsomedial superior temporal area (MSTd), parietal area 7a, and dorsolateral prefrontal cortex (dlPFC) as monkeys navigate in virtual reality to “catch fireflies”. This task requires animals to actively sample from a closed-loop visual environment while concurrently computing latent variables: the evolving distance and angle to a memorized firefly. We observed mixed selectivity in all areas, with even a traditionally sensory area (MSTd) tracking latent variables. Strikingly, global encoding profiles and unit-to-unit coupling suggested a functional subnetwork between MSTd and dlPFC, and not between these areas and 7a, as anatomy would suggest. When sensory evidence was rendered scarce, lateral connectivity through neuron-to-neuron coupling within MSTd strengthened but its pattern remained fixed, while neuronal coupling adaptively remapped within 7a and dlPFC. The larger the remapping in 7a/dlPFC and the greater the stability within MSTd, the less was behavior impacted by loss of sensory evidence. These results highlight the distributed nature of neural coding during closed-loop action-perception naturalistic behaviors and suggest internal models may be housed in the pattern of fine-grain lateral connectivity within parietal and frontal cortices.