bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroprotective effect of Ziziphi Spinosae Semen on p-chlorophenylalanine induced insomnia rats by activating GABA A receptor
Authors: Xiao Fengqin , Shao Shuai , Zhang Hongyin , Li Guangfu , Piao Songlan , Zhao Daqing , Li Guangzhe , Yan Mingming 
Published: May 2022

Abstract
Ziziphi Spinosae Semen (ZSS), as the first choice of traditional Chinese medicine in the treatment of insomnia, has attracted extensive attention. However, the current studies on improving sleep mechanism of ZSS are mainly focused on the role of single component, to further reveal the potential mechanism of ZSS in multi-constituents is necessary. In this study, ZSS extract (ZSSE) was extracted from ZSS via detailed modern extraction, separation and purification technologies. The chemical constituents of ZSSE were analyzed by HPLC-MS. In vivo experiments, rat’s model induced by p-chlorophenylalanine (PCPA) has been established to investigate the potential effect and corresponding mechanism of ZSSE on improving sleep. HE stains experimental results indicated that the drug group showed fascinating advantages over model group in improving sleep. Moreover, the levels of GABA, Glu, 5-HT and DA factors of insomnia rats in brain were also monitored via ELISA method to further study the improving sleep mechanism of ZSSE. The results indicate that sleep could be improved effectively via up-regulation of GABA and 5-HT, and down-regulation of Glu and DA, respectively. In addition, molecular mechanisms of ZSSE in improving sleep were also studied by immunohistochemical analysis. The results indicate sleep could be improved by regulating the expression levels of GABAARα1 and GABAARγ2 receptors in hypothalamus and hippocampus tissue sections. Therefore, this work not only identifies the active ingredients of ZSSE, but also reveals the potential pharmacological mechanism of ZSSE for the improving sleep, which may greatly stimulate the prospective development and application of ZSSE.
Effect of Oral Motor Facilitation Technique on Oral Motor and Feeding Skills in Children with Cerebral Palsy : A Case study
Authors: Min Kyoung-chul , Seo Sang-min , Woo Hee-soon 
Published: May 2022

Abstract
Background : Deficiencies in oral motor and feeding skills are common in children with cerebral palsy (CP). Oral motor therapy is a useful method to improve oral motor skills and feeding skills. The oral motor facilitation technique (OMFT) is a newly designed oral motor therapy that includes postural control, sensory adaptation, breathing control, sensorimotor facilitation, and direct feeding. Methods : This study was performed to determine the effect of OMFT on oral motor and feeding skills in children with CP. A total of 21 children with CP (3–10 years, GMFCS III–V) participated in 16 sessions of OMFT for 30 min, once a week for 16 weeks. The effects on oral motor and feeding skills were assessed using the Oral Motor Assessment Scale (OMAS) before as well as 8 and 16 weeks after treatment. Data were analyzed using the Friedman test and post-hoc analysis. Results : The results showed a significant improvement in oral motor skills and feeding skills, including mouth closure, lip closure on the utensil, lip closure during deglutition, control of the food during swallowing, mastication, straw suction, and control of liquid during deglutition after OMFT. Mouth closure was the most effective and mastication was the least effective item. Both the post-training periods of 8 and 16 weeks were effective, with the latter being more effective than the former. Conclusions : The results suggest that OMFT is an extremely effective and useful oral motor therapy protocol to improve oral motor function in children with CP.
Decreased Sirt3 contributes to cyclic production of ROS and islet β-cell apoptosis in high glucose conditions
Authors: Cai Zhichen , Liu Shuqing , Nie Yaxing , Dong Bingzheng , Li Chenglin , Zhang Jinyuan , Xia Chunya , Du Lei , Yin Xiaoxing , Wang Jianyun 
Published: May 2022

Abstract
Background Reactive oxygen species (ROS) plays a vital role in the apoptosis of islet β-cells in T2DM. Sirt3 and FoxO1 might be involved in ROS production. This study was to investigate mechanism of ROS production and β-cell apoptosis.Methods Oxidative stress and apoptosis in islet of db/db mice and high glucose cultured β-cells were observed by TUNEL assay and western blotting. Then, H2O2 was used to ascertain the effect of ROS on the expression of Sirt3. Meanwhile, FoxO1, antioxidant enzymes (MnSOD and CAT) and β-cell apoptosis were also determined by western blotting. Finally, Sirt3 was knocked down to evaluate the effect on oxidative stress and apoptosis of β-cells.Results Under high glucose environment, enhanced ROS made a decrease of Sirt3 expression, which increased acetylation of FoxO1, thus reduced the expression of its target proteins --MnSOD and CAT, and further significantly increased ROS levels. Increased ROS further led to the apoptosis of β-cells. Conclusion Down-regulation of Sirt3 played an important role in the cyclic production of ROS. Targeting Sirt3 may be favorable for the treatment of T2DM.
Prediction of Coastal Hydrodynamics Quarantined in the Sediments With Utility of Textural Characteristics and Magnetic Susceptibility of Jagatsinghpur Coast, Odhisa District, India
Authors: Sivaprakasam Vasudevan , G SATHIYAMOORTHY , R Selvaganapathi , Palani Balamurugan , S Baranidharan 
Published: May 2022

Abstract
This current research aims to perceive depositional energy environment of jagatsinghpur coastal area sediments with outcome of Textural aspects of particle size dissemination and Magnetic susceptibility (MS). In study, 36 sediment samples recovered from 12 locations with 3 points such as Low Tide (LT), High Tide (HT), and Berm (BM) along with every 5 km interval. To observe outcome from different parameters such as Mean (MQ), Standard deviation (SD), Skewness (SK) & Kurtosis (KU), bivariate plots, and MS methods were adopted. From the outcome, the MQ and SD of LT, HT and BM infer medium sand to fine sand with a moderately sorted to well-sorted nature as well as SK and KU of LT, HT and BM infer extreme coarser skewed to fine skewed with platykurtic to extreme leptokurtic nature. Textural parameters shows the Southern Part of the study area experienced the low energy condition. Mainly, the energy process plot indicates sediments fall in the inner shelf and river environment. CM plot expressed the sediment samples reacted as graded suspension and rolling mechanism. The MS of low frequency, the higher quantity of magnetic contents of 3 zones low tide (LT), high tide (HT), and berm (BM) has been 62.57 x10− 8 m3 kg− 3, 72.83 x10− 8 m3 kg− 3 and 454.63 x10− 8 m3 kg− 3. The Pearson’s Correlation signifies the + ve relationship between Berm low frequency vs Berm Mean, low tide means (r2 = 0.57, (r2 = 0.40) signifies the multi-source of sediment deposition. From the output, the northern part of study infers high energy environment.
Plasmonically induced transparency peaks fluctuation within multi-rectangle resonators
Authors: Pei Ruoyu , Liu Dongdong , Zhang Qun , Shi Zhe , Sun Yan , Liu Xi , Wang Jicheng 
Published: May 2022

Abstract
Plasmonically induced transparency (PIT) effect in a metal–insulator–metal waveguide coupled to asymmetric three rectangle resonators and an appearance that one PIT peak fall while the other PIT peak rise are investigated numerically. PIT is widely studied duo to the performance on sensing, slow slight and nonlinear effects, which has great potential to be applied on optical communication system. To get a better insight of PIT effect in multi-rectangle resonators, herein, PIT peaks fluctuation is employed to numerically investigate its corresponding properties, effects and performance. By modify geometric parameters and filling dielectric, not only the off-to-on PIT optical response within single or double peaks can be realized, but also the fluctuation of peaks can be achieved as well. Additionally, our findings are well consistent with finite element simulation outcomes. These proposed structures also possess great potential in both communication and sensing applications.
Multisystem Inflammatory Syndrome in children: tools for a timely diagnosis in the Emergency Department from an Italian Multicenter Survey
Authors: Mauro Angela , Maglione Marco , Savoia Fabio , Calvi Matteo , Amoroso Angela , Sangerardi Maria , Tubino Barbara , Piccotti Emanuela , Fabi Marianna , Salvadeei Serena , Gadda Dario , Marchetti Federico , Midulla Fabio , Buonsenso Danilo , Magrassi Silvia , Salvini Filippo Maria , Pignataro Rossana , Florio Francesca Di , Giudice Emanuela Del , Lubrano Riccardo , Tipo Vincenzo , Rosa Mrgherita , Gagliardi Thaililja , Picciano Ludovica , Stanco Michela , Gagliardi Stefania , Orlando Francesca , Ballani Iacopo , Canzaniga Viola , Medici Francesco , Torre Francesco La , Cardinale Fabio , Tibaldi Jessica , Schiappapietra Benedetta , D'Alessandro Matteo , Bitelli Maria , Gallotta Giulia , Masi Stefano , Nucci Alessia , Agosti Massimo , Plebani Annamaria , Iacono Alessandra , Cairello Francesca , Ghizzi Chiara , Rossi Nadia , Schiavone Iolanda , Gentile Antonia 
Published: May 2022

Abstract
Objective To assess the most frequent clinical features of Multisystem Inflammatory Syndrome in children (MIS-C) at presentation to the Emergency Department (ED) in a large multicenter cohort of patients, in order to define useful tools for a timely diagnosis.MethodsClinical and laboratory characteristics were retrospectively reviewed for 210 MIS-C patients from 18 Italian pediatric EDs. We assessed correlations between clinical and laboratory parameters and compared features of patients of different age (≤5 years and >5 years). ResultsFever was the main presenting symptom (100%), followed by conjunctivitis (46%), abdominal pain (44%), vomit (41%) and diarrhea (39%). Forty-nine percent of children presented to the ED in critical or nearly critical conditions. A higher prevalence of mucocutaneous involvement was found in younger children (69% versus 47%, p<0.05), whereas gastrointestinal symptoms were more common in children >5 years (62% versus 85%, p<0.05). Higher values of inflammatory markers (C-Reactive Protein, Ferritin, and Fibrinogen), Troponin T and Brain Natriuretic Peptide were related to abnormal echocardiography (p<0.05). No significant differences were detected in laboratory parameters between the two age groups, apart from ferritin, fibrinogen and troponin T, which resulted significantly lower in children ≤5 years.ConclusionsApart from fever, most common MIS-C manifestations at presentation to the ED are conjunctivitis, abdominal pain, vomit and diarrhea. Younger children more frequently present with mucocutaneous involvement, while gastrointestinal manifestations are more common in older patients. These findings should be considered when MIS-C is suspected in the ED, in order to achieve a timely recognition of the condition.
Development of an insilico model of eccrine sweat using molecular modelling techniques
Authors: Deshpande Parijat , Ravikumar Bharath , Tallur Siddharth , Paul Debjani , Rai Beena 
Published: May 2022

Abstract
Exploring eccrine sweat as an ideal surrogate diagnostic biofluid for physiological and metabolic biomarkers, which are indicators of human health and performance, is an area of significant research interest. These biomarkers correlate well with blood plasma and candidate analytes such as glucose which require periodic monitoring are of particular interest. An insilico model of eccrine sweat can assist in the development these biosensors. In this regard, molecular modelling can be employed to observe the most fundamental interactions. Here, we determine a suitable molecular model for building eccrine sweat. The basic components of sweat are water and sodium chloride, in which glucose and other analytes are present in trace quantities. Given the wide range of water models available in the molecular dynamics space, in this study, we first validate the water models. We use three compounds to represent the base to build bulk sweat fluid and validate the force fields. We compare the self-diffusivity of water, glucose, sodium, and chloride ions as well as bulk viscosity values with the existing literature. This validated model is made available and can serve as an aid for de novo development of biosensors by further addition of other analytes via simulations to expedite their development.
A New Gene Therapy for Pancreatic Cancer: STAT6-CYBB Decoy Oligodeoxynucleotides Focusing on Inhibiting M2 Macrophages
Authors: Chen Chang-Jung , Wang Hao-Chen , Hou Ya-Chin , Wu Yi-Ying , Shieh Chi-Chang , Shan Yan-Shen 
Published: May 2022

Abstract
Background: M2 tumor-associated macrophages can promote pancreatic cancer progression. Under ROS, cancer cells derived IL-4 can skew M2 with uncertain mechanism. In this study, we tried to elucidate the mechanism and design gene therapy to treat pancreatic cancer by inhibiting M2 macrophages. Methods: We used CRISPR/Cas9 to knock out CYBB that encodes NOX2 in macrophages and adopted CYBB-/- B6 mice. Co-culture assay and syngeneic orthotopic pancreatic cancer mice models were used to study the mechanism in skewing M2 and tumor growth. The decoy oligodeoxynucleotides were designed to inhibit M2 formation and control the tumor growth. Results: Co-culture with pancreatic cancer cells induced M2 polarization at 72 h, while CYBB knockout prevented it. In the syngeneic orthotopic model, M2 macrophages count was decreased and tumor formation was inhibited in Cybb-/- mice. Under secretion of ROS in activated macrophages, cancer cell derived IL-4 can increase CD204 expression on macrophages. Chromatin immunoprecipitation study showed a binding site of STAT6 and HDAC2 in the promotor region of Cybb gene. Using epigenetic inhibitors or knocking down STAT6 and HDAC2 could block the M2 skewing with reducing the IL-10 expression and keep on M1 macrophages with increasing the IL-12 expression. Gene therapy using STAT6-CYBB decoy oligodeoxynucleotides to interrupt STAT6/HDAC2 binding to CYBB promoter could inhibit M2 formation and reduce tumor growth significantly.Conclusion: Gene therapy using STAT6-CYBB decoy ODN can inhibit M2 formation to treat pancreatic cancer.
Waterborne diseases as an indicator of health disparities: A nationwide study of WaSH related morbidity and mortality in Ecuador from 2011-2020
Authors: Ortiz-Prado Esteban , Simbaña-Rivera Katherine , Cevallos Gabriel , Gómez-Barreno Lenin , Guerrero Jhon , Ayala Lesly , Cevallos Domenica , Lister Alex , Fernandez-Naranjo Raul , Ríos-Touma Blanca , Vasconez Jorge , Izquierdo-Condoy Juan S. 
Published: May 2022

Abstract
BackgroundDespite worldwide progress in terms of clean water supply, sanitation, and hygiene knowledge, some middle and most of low-income countries are still experiencing many diseases transmitted using unsafe water and the lack of sanitationMethodsTo understand the impact of all waterborne diseases (WBD) registered in Ecuador, we performed an analysis of all cases and deaths related to WBD to compute incidence and mortality rates.ResultsWe found that in Ecuador, mestizo people had the greatest morbidity rate (141/100,000) patient followed by indigenous (63/100,000) and self-determined white patients (21/100,000). However, in terms of mortality, indigenous population have a 790% increase in mortality rate (2.6 /100,000) when compared to self-determined white populations (0.29/100,000) or 176% more when compared to mestizos (0.94/100,000). This trend remains the same among children and the elderly who have higher mortality rates when compared to other ethnic groups.ConclusionsIn Ecuador, water borne diseases (WBD) are still a major public health problem. We found that younger children and elderly are more likely to be get sick and die due to water borne diseases. In terms of morbidity, mestizos reported the highest rate, while in terms of mortality, indigenous populations are the most affected, having the highest mortality among different ethnic groups. We hypostatize that reduced health care access is linked to fewer reporting incidence rates among indigenous populations but higher mortality rates.
The serum creatinine to cystatin C ratio predicts the risk of acute exacerbation of chronic obstructive pulmonary disease
Authors: Lei Ling , Li Yan , Gou Xijun , He Liang 
Published: May 2022

Abstract
Background: The aims of acute exacerbation of chronic obstructive pulmonary disease (AECOPD) treatment are to minimize the negative impact of the current exacerbation and to prevent the development of subsequent events. Hence, it is important to identify serological indicators that are readily available at the time of hospital admission to assess the prognosis of patients with AECOPD. Methods: All patients hospitalized in a Department of Respiratory and Critical Care Medicine of tertiary care hospital between January 2021 and December 2021 for AECOPD were analyzed using univariable correlations and binary logistic regression analysis with two models for associations between demographic, clinical and laboratory features and AECOPD risk.Results: The Cre/Cys C ratio was significantly associated with age (r = –0.206, P = 0.000), weight (r = 0.331, P = 0.000), BMI (r = 0.133, P = 0.007), and FVC% predicted (r = 0.130, P = 0.009). Multiple regression was run to predict the Cre/Cys C ratio from age, weight, BMI, FEV1/FVC ratio and FVC% predicted FABP-4, with F (5, 405) = 24.571, P=0.000, R2 = 0.233. The results showed that the most significant predictors of the Cre/Cys C ratio were age (P=0.007), weight (P=0.000), BMI (P=0.000), and predicted FEV1 (P=0.000). Multivariate analysis was performed to determine whether the Cre/Cys C ratio was a predictor of AECOPD risk. Model 1 showed that a low Cre/Cys C ratio was associated with an increased hospital LOS (odds ratio (OR): -0.114, 95% confidence interval (CI): -0.061- -0.005) and admission to the ICU (OR: 0.951, 95% CI: 0.907-0.996). After adjustment for potential confounding factors, model 2 showed that a low Cre/Cys C ratio was not independently associated with AECOPD risk.Conclusion: The present study indicated that the Cre/Cys C ratio is an easy, cheap, repeatable and promising tool that allows us to evaluate the risk of AECOPD using serum markers. A low Cre/Cys C ratio was associated with a prolonged LOS and admission to the ICU in AECOPD patients. However, the associations were not independent.
PI3K/AKT and SHH singling pathway contribute to metastasis relapsed during gene evolution in human Oligodendroglioma
Authors: Liu Guanzheng , Bu Chaojie , Guo Guangzhong , Zhang Zhiyue , Sheng Zhiyuan , Deng Kaiyuan , Wu Shuang , Xu Sensen , Bu Yage , Gao Yushuai , Wang Meiyun , Liu Gang , Kong Lingfei , Li Tianxiao , Li Ming , Bu Xingyao 
Published: May 2022

Abstract
Background: Oligodendroglioma shows a relatively better prognosis, responds well to radiotherapy and chemotherapy, and rarely progress to a very aggressive tumor. Little is known about the evolution of genetic and epigenetic changes that occur in the progression of oligodendroglioma. Methods: We evaluated gene evolution changes in progressive relapsed oligodendroglioma based on deep whole-genome-sequencing data(ctDNA). We analyzed longitudinal genomic and molecular data from 6 patients under therapy. Multiple metastasis recurrence occurred in 5 cases.Results: Whole-exome sequencing demonstrated that the rate of shared mutation between the primary tumors and recurrent tumors or TISF was relatively low. In two cases, even well-known presumptive major driver mutations of CIC and FUBP1 that were detected in primary tumors were not detected at relapsed TISF. Among them, 2 cases converted into IDH1 wild state in the process of gene evolution under stress treatment, indicating that the cell phenotype and genetic characteristics of oligodendroglioma will change in the process of tumor evolution, and metastasis relapsed is a later event. Two patients underwent long-term chemotherapy before operation, we find that recurrence tumors harbor mutations in PI3K/AKT and sonic hedgehog(SHH) singling pathways. One case showed hypermutation when it recurred, with a clear mutational signature, lead to the rapid progress of the tumor. Conclusions: Oligodendroglioma has great spatial and temporal heterogeneity during gene evolution, lead to the rapid progress of the tumor. Metastasis relapsed is a later event during oligodendroglioma progression. PI3K/AKT and SHH signaling pathway plays an important role in promoting tumor metastasis and drug resistance during the relapsed of oligodendrocytes. Cell phenotype and genetic characteristics of oligodendroglioma will also change in the process of tumor evolution, tendency to increase the malignant degree of the tumor. Trial registration: The report was retrospectively registered in Zhengzhou University People’s Hospital, and the registration number is 2122432055 and date of registration should be in March, 2021.
Analyzing the Reliability of the Grouping-Based Concept Lattice Reductions and a Method for Improving It
Authors: Peng Siqi , Yamamoto Akihiro 
Published: May 2022

Abstract
Formal concept analysis (FCA) is an important task for analyzing rela-tional data as well as extracting formalized knowledge from them. It has a wide range of practical applications, while a problem is frequently encountered that the extracted knowledge, i.e., the concept lattice may be too complicated for further processing and/or analysis. To reduce the concept lattice, various branches of techniques have been proposed and one of the most preferred categories of methods is the grouping-based simplification methods which aim at reducing the volume of the concept lattice by removing the redundant objects/attributes. However, we figure out that these methods may introduce too much information distortion to the context and the concept lattice, making the reduction result unreliable. To identify and overcome these problems, we propose a method for analyzing the reliability of a reduction in terms of context fidelity and lattice fidelity as well as a novel grouping-based concept lattice reduction method using an integer linear programming model. We conduct experiments on several data sets to prove that our method works in different cases and can produce more reliable reductions.