bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors driving poor health and influencing unhealthy behaviour uptake in deprived areas.
Authors: Stockton James , Lui Steve , Halsall Jamie P , Ndzogoue Nadege Pouandeu 
Published: October 2022

Abstract
Background

Better understanding health determinants affecting poor health outcomes and influencing unhealthy behaviour is increasingly considered an important focus. This study investigated participants’ Healthy Eating and Active Living (HEAL) behaviours, as well as the attitudes, thoughts and experiences, associated with the Social Ecological Model (SEM) [1] that influenced said health behaviours.

Method

We used HEAL and SEM to identify the uptake and maintenance of modifiable behaviours affecting physical activity, diet, and long-term behaviour (i.e. smoking and alcohol consumption). Participants were collected for 13 focus groups (N=97: Female = 78 and Male
= 19) and 15 one-to-one interviews (N=15: Female = 9 and Male = 6). All data gathered during focus groups and interviews was recorded and transcribed. Thematic analysis (Braun and Clarke, 2019) was used to contextualise the data and develop themes in line with SEM. Recruitment of participants occurred in a Northern English town location was selected based on previously identified large amount of health disparities occurring in research location.

Results

Four themes were identified: healthy eating and sensible drinking awareness, unhealthy food and alcohol consumption affected by outlets density, environmental impact upon mental health, and attitudes towards government interventions. SEM examined the effectiveness of
community level interventions whilst considering the individual responsibility associated with HEAL.

Conclusion

Inherited attitudes towards HEAL are deeply impacting participants. Inter-generational transferal of beliefs arising from a lack of HEAL is likely to remain an uphill struggle. Health determinants remain a concern, with many deprived areas within England suffering from worse health than non-deprived areas. Within these deprived areas, barriers to health exist at multiple levels and are encountered throughout an individual’s life. National interventions provided good information; however, they are either hampered by a lack of effective dissemination to the general population, or lack the key information required. Local interventions often have more success in effectively engaging local people, but transient local interventions eventually disappear due to a lack of long-term funding. We provide some evidence to support the notion that Non-Communicable Diseases (NCD) health interventions should be provided in a local and sustainable fashion.
An ethnobotanical study on wild plants used by Tibetan people in Gyirong valley, Tibet, China
Authors: Guo Chang-An , Ding Xiaoyong , Hu Huabin , Zhang Yu , Yang Huizhao , Wang Yuhua 
Published: October 2022

Abstract
Background: Gyirong valley is known as the “Back Garden of the Himalayas” and is located in the core area of the Everest National Nature Reserve. Gyirong is also one of the important ports from ancient Tibet to Kathmandu, Nepal. Over the years, the Tibetans of Gyirong had accumulated sufficient traditional knowledge about local plant resources. However, there is almost no comprehensive report available on ethnobotanical knowledge about the local people. The purposes of this study were to (1) conduct a comprehensive study of WEPs used by Tibetan people in Gyirong valley, document the traditional knowledge associated with wild useful plants, and (2) evaluate species with important cultural significance to Tibetan people of Gyirong Valley.
Methods: Ethnobotanical data were documented through free listings, key informant interviews, and semi-structured interviews during fieldwork. The culture importance index (CI), and the informant consensus factor index (FIC) were used as quantitative indices.
Results: In total, 120 informants (61 women and 59 men) and 3,333 use reports and 111 wild plant species belonging to 39 families and 81 genera were included. These use reports were then classified into 27 categories belonging to 3 major categories. The use category that contained the most plant species was edible plants (62), followed by medicinal plants (32), and other uses (71). Plants with high CI included Allium prattii, Neopicrorhiza scrophulariiflora, Gymnadenia orchidis, Rhododendron anthopogon, Fritillaria cirrhosa. There are 36 species of plants in the catalog of Gyirong and Yadong were the same, but only 17 species were the same in Gyirong and Burang. There were only 11 overlapping species between all the three regions.
Conclusion: Tibetans of Gyirong have rich and unique knowledge about plant use, and wild edible and medicinal plants play an important role in the nutrition and health protection of local people. There is a higher proportion of ritual plants and wooden bowl handicraft plants in Gyirong. However, traditional knowledge is slowly being lost and is being hit by modern tourism. In the future, more attention needs to be paid to the important role of traditional knowledge in biodiversity conservation.
The Great Synergistic Performance for Adjunction of ACR TI-RADS and Color Flow Imaging Vascularity Scoring in Predicting Malignant Thyroid Nodules
Authors: Khider Mohamed Omer , Ayad Caroline Edward , Gareeballah Awadia , Alshoabi Sultan Abdulwadoud , Gameraddin Moawia , Elzaki Maisa , Alsharif Walaa , Alsultan Kamal D. 
Published: October 2022

Abstract
Purpose: To investigate the diagnostic performance of Color Doppler Flow Imaging (CFI) vascularity score in comparison to the ACR TI-RADS for prediction of malignancy in thyroid nodules (TNs).
Materials and Methods: A cross-sectional prospective study design was used. Patients underwent thyroid ultrasound imaging for TNs which classified the TNs using ACR TI-RADS and CFI vascularity score. Receiver operating characteristic (ROC) analysis was applied to compare between the CFI vascularity score and ACR TI-RADS.
Results:A total of 222 patients participated in this study, 76.6% of them were female, with a mean age of 44.73 ± 13.31 years (range, 18 to 85). Ultrasound imaging classified TNs as: (TI-RADS 4= 41%), (TI-RADS 5=30.6%), and (TI-RADS 3= 28.4%). TNs of TIRADS 3, 4, and 5 and with CFI vascularity score category 1 were100% benign, and TNs of TIRADS 3, 4, and 5 and with CFI vascularity score category 4 were malignant in 82.5%, 91.3%, 100%, respectively, (p<0.001). A significant difference in the area under ROC curve among CFI vascularity scores and ACR TI-RADS was found. CFI vascularity showed sensitivity of 85.2%, specificity of 86.8%, PPV of 86% and NPV of 86.1%, in prediction malignancy in TNs, where CFI scores>2. However, the ACR TI-RADS showed sensitivity 57.4%, specificity 94.7%, PPV 91.2% and NPV 70.1% in prediction malignancy, in ACR TI-RADS ≥ 4, (p<0.001).
Conclusion:Combining CFI vascularity score and TI-RADS can be consider as a safe and non-invasive diagnostic method for accurate risk stratification of TNs and can resolve the gap in follicular TNs.
Identification and Validation of Hub Genes and Pathways in the Stenosis of Arteriovenous Fistula
Authors: Li Yu , Cui Wenhao , Wang Jukun , Chen Xin , Zhang Chao , Zhu Linzhong , Bian Chunjing , Luo Tao 
Published: October 2022

Abstract
Background and Purpose
Arteriovenous fistula (AVF) is the most widely used hemodialysis vascular access in China. However, stenosis of AVF limited its use. Moreover, the mechanism of AVF stenosis is currently unknown. Therefore, the purpose of our study is to explore the mechanisms of AVF stenosis.
Materials and Methods
In this study, we identified differentially expressed genes (DEGs) based on Gene Expression Omnibus (GEO) dataset (GSE39488) between venous segments of overflowing of AVF and normal veins. Gene set enrichment analysis (GSEA) were performed for the functional annotation of DEGs. A protein-protein interaction (PPI) network was created to identify the hub genes of AVF stenosis. Combining the results of GSEA and PPI network analysis, along with analyses from other literatures, FOS and NR4A2 were selected as the target genes to be further investigated. Finally, we validated the bioinformatics results with reverse transcription-PCR (RT-PCR) and western blot analysis on human and rat samples, respectively.
Results
Six hub genes (FOS, NR4A2, EGR2, CXCR4, ATF3, and SERPINE1) were found by bioinformatics analysis. Additionally, the expression levels of mRNA and protein of FOS and NR4A2 were upregulated in both human and rat samples.
Conclusions
In summary, the results suggested that FOS may play an important role in AVF stenosis by activating MAPK signaling pathway, which could be a potential therapeutic target of AVF stenosis.
Does the addition of luteal function support drug benefit pregnancy and perinatal outcome in modified natural cycle frozen thawed embryo transfer in women under the age of 35- year-old?
Authors: Zhang Wen , Ren Bingnan , Wu Sheling , Zhang Wenjuan , Guan Yichun , Du Xiaofang , wang bijun 
Published: October 2022

Abstract
Background:In the freeze-thaw embryo transfer, the use of best luteal phase support protocols is continuously being discussed, especially in the natural cycles. Previous comparisons of luteal phase support protocols in natural cycles focused on pregnancy outcome, with less attention to perinatal outcome. Thus, convincing conclusion about which protocol is more appropriate could not be drawn. At the same time, with increasing luteal support drugs, the benefit of using these drugs also needs evidence.
Methods: The clinical data of 3658 mNC-FET cycles in the Reproductive Center of the Third Affiliated Hospital of Zhengzhou University from January 2018 to December 2020 with the participants’ age of less than or equal to 35 years old，were retrospectively analyzed. The cycles were divided into three groups based on luteal phase support protocols used. The patients received a combination of progesterone soft capsule and dydrogesterone in the group A (882 cycles), the patients received dydrogesterone in the group B (627cycles), and the patients received a combination of progesterone vaginal sustained-release gel and dydrogesterone in the group C (2149 cycles). Pregnancy and perinatal outcomes in the group A, group B, and group C were compared.
Results: HCG positive rate, clinical pregnancy rate, and implantation rate were higher in the group B than in the groups A and C （67.30% vs. 57.37% and 61.05%; 64.43% vs. 55.67 % and 57.88 %; 54.83 % vs 42.70 % and 45.92 %）. The 12-week pregnancy rate and live birth rate in the group B were higher than those in the group A （56.30% vs. 49.55%；53.26% vs. 46.83%）. There were no significant differences in newborn weight, premature delivery rate, pregnancy complications rate, and incidence of birth defects among the three groups. Logistic regression analyses showed that three different luteal phase support regimens were not associated with live birth rate （0R 95% CI B VS. A=1.23，P=0.056；0R 95%CI B VS. A=1.07，P=0.374）.
Conclusions: In the mNC-FET cycle, patients younger than or equal to 35 years of age, who chose dydrogesterone alone as luteal support drug, had no difference in live birth rate and perinatal outcome between progesterone soft capsules or progesterone vaginal sustained-release gel combined with dydrogesterone, but the outcome still needs to be confirmed by large sample prospective studies.
Genetic Diversity of Avocado (Persea americana Mill) From Southern Ethiopia Using SSR Markers
Authors: Degu Hewan Demissie , Tadesse Almaz , Kalousova Marie 
Published: October 2022

Abstract
Background: Avocados are a cash crop in Ethiopia. However, its genetic diversity has not been investigated. This study explored the genetic diversity of avocados in southern Ethiopia using SSR markers.
Method and Result: One hundred nine avocado trees were sampled in sixteen districts. One hundred forty alleles were detected across the 12 loci. The average number of alleles per locus was 11.7. The average expected and observed heterozygosity was 0.63 ± 0.12 and 0.48 ±0.19, respectively. All loci showed a significant deviation from HWE. The analysis of molecular variance showed that about 5% of the variation was among the sixteen populations. Pairwise comparisons of population FST values revealed a lack of genetic differentiation for seven out of 48 paired comparisons. UPGMA based on Nie and Li similarity index, classified the genotypes into three major classes with 100 and 53 bootstrap values. Genetic distance analyses showed a mixing of avocado trees from different districts. DAPC divided the tree samples into three groups, while the model-based STRUCTURE subdivided the tree samples into two major genetic clusters.
Conclusion: Moderate diversity was detected in the analyzed avocado germplasm. These imply, the germplasms are a valuable source of alleles for genetic improvement in Ethiopia. The mixing of avocado trees from different districts observed in UPGMA tree, DAPC, and STRUCTURE analysis. This indicated strong gene flow among the avocado populations. There is, however, significant variation among the tree populations from different districts to be used in the avocado breeding program.
Ecological niche modelling approach for climate suitability of spineless cactus pear (Opuntia ficus-indica (L.) Mill.) in semi-arid and arid regions of India
Authors: Kumar Sunil , Dikshit Nilamani , Sivaraj Natarajan , Govindasamy Prabhu , Palsaniya Dana Ram , Louhaichi Mounir , Hassan Sawan , Liguori Giorgia , Kumar Prashant 
Published: October 2022

Abstract
Spineless cactus pear [Opuntia ficus-indica (L.) Mill.] is a promising subtropical non-conventional fodder crop in India. Ecological niche modelling studies using the maximum entropy approach has been used to assess the suitable cactus growing regions in India. The dataset for cactus pear presence locations was obtained from various centres. WorldClim dataset representing current and future climate was downloaded from http:// www.worldclim.org. The cactus presence locations dataset and WorldClim dataset were used with Maximum entropy (MaxEnt) modelling to generate the climate suitability map to show potential growing sites in India. The generated maps indicated that potential regions for the cultivation of cactus exist in several states of India viz., Andhra Pradesh, Bihar, Delhi, Haryana, Karnataka, Madhya Pradesh, Maharashtra, Odisha, and Uttar Pradesh. The outcome of the study would help in managing cactus pear genetic resources in the country to meet the challenges of climate change and fodder production.
Alternating iterative coupling of hydrological and hydrodynamic models applied to Lingjiang river basin, China
Authors: Liu Miao , Ding Yongsheng , Shen Zeyu , Kong Qiao 
Published: October 2022

Abstract

The increasing frequency of extreme-weather flood events is attributable to climate change. In addition to constructing basic water conservancy facilities, predicting the flood inundation range is important for preemptively reducing the flood risk and guiding the formulation of government policies. Conventionally, flood routing simulation based on two-dimensional hydrodynamic simulation is applied. However, the hydrological method has inherent advantages, which can be leveraged to realize information exchange. In 2019, Typhoon Lekima caused a severe flood disaster in Taizhou, China and large-scale inundation in residential areas. In this study, this event is hydrologically and hydrodynamically modeled using HEC-WAT and other HEC platform software. Loose coupling and tight coupling are conducted via different coupling methods, and the differences between the two modeling methods are compared. It is found that tight coupling utilizes the dynamic interaction mechanism of the hydrological and hydrodynamic models, which reduces the simulation error. Similarly, the flood problem in the Taizhou area is analyzed. The two-dimensional flood routing simulation results show that the flood flows in the plain areas along the bank are within the flood area, the maximum depth of inundation in the area reaches 17.0 m, and the water level reaches 11 m. The comparative simulations show that the alternating iterative coupling of hydrological and hydrodynamic can improve the accuracy of the model simulation results.
IL-37 regulates glycolysis by inhibiting GLUT1 expression in lung adenocarcinoma
Authors: Zhao Li , Wang Peng , Chen Wen , Zhu Mingxue , Wang Yushan , Ma Cuiping , Mu Xiaofeng , Wang Ye 
Published: October 2022

Abstract
Aerobic glycolysis is the most classical pathway in tumor metabolic reprogramming. Aerobic glycolysis has been found in several studies to have an essential role in lung adenocarcinoma progression. Interleukin 37 (IL-37) has an important protective role in lung cancer. The mechanism by which glycolysis in lung adenocarcinoma is affected by IL-37 has not been clarified. Using qRT-PCR and western blot, we uncovered that GLUT1 expression was significantly higher in A549 cells than in BEAS-2B cells. GLUT1 expression was decreased in A549 cells treated with rhIL-37. Also, glucose uptake and lactic acid production were significantly reduced in A549 cells treated with 6uM BAY-876 (GLUT1 specific inhibitor). GLUT1 expression in A549 cells in this study was not inhibited by intracellular overexpression of IL-37. Glucose uptake and lactic acid production may be inhibited by extracellular IL-37 by inhibiting GLUT1 expression. Therefore, IL-37 has a role in glucose metabolism in lung adenocarcinoma cells.
Reliability of Clinical Examination Methods for Pain in Periapical Periodontitis
Authors: Eyüboğlu Tan Firat , Kim Hyeon Cheol 
Published: October 2022

Abstract
Objectives: This study aimed to compare pain intensity levels of a tooth at rest and in function, and percussion and chewingtests to verify a reliable clinical test method.
Materials and Methods: A total of 36 asymptomatic necrotic teeth that required root canal treatment, one in each patient, were included. The entire treatment procedure was performed during a single visit by an experienced endodontist. Patients were asked to mark their pain levels on a vertical visual analog scale (VAS) while the relevant tooth was at rest and during function 24 h after the treatment. In addition, patients were asked to mark their pain levels on the same VAS after the clinical tests of percussion and chewing. Finally, the pain levels were compared using Pearson’s correlation for the reliability of the test methods at a significance level of 95%.
Results: The postoperative pain levels measured during the clinical tests and functions were significantly higher than the pain levels at rest (p<0.05). The pain levels by percussion were significantly higher than that during the function and chewing tests (p<0.05). Pain intensity during the function was simulated with a higher correlation using the chewing strip method rather than the percussion method.
Conclusions: According to this study, a bite test using chewing strips as a pain intensity assessment can mimic the actual postoperative pain experience, whereas percussion tests fail.
Clinical relevance: The pain intensity levels determined by the bite test using chewing strips may provide reliable clinical signs similar to actual pain levels.
Trends in the quality of care for acute chest pain after chest pain center accreditation
Authors: Hou Shuang , An Ning , Liu Meina 
Published: October 2022

Abstract
Background:Chest pain centers (CPCs) have become important for improving the quality of care for acute chest pain. However, no study has shown whether adherence to guidelines continuously improvesin CPCs after certification.
Objectives:We aimed to analyze trends in quality indicator (QI) compliance rates of Chinese CPCs between January 1, 2016 and December 31, 2018.
Methods: We drew a time series stacked area plot of compliance rates forQIs to describe the change in their cumulative value over time. Next, we selected the time points with the most remarkable change in the stacked area plot as the breakpoints and constructed an interrupted time series (ITS) design for analysis. Significant ITS results indicated that the breakpoints had effects on the trend. We then estimated the average monthly percent change (AMPC) using joinpoint regression analysis.
Results: We found an unexpected downward trend in the cumulative compliance rates for the nine QIs assessed between the 12thand 13th months and between the 24thand 25th months. The trends of QI1‒5 showed a continuously increasing change. QI6‒8 showed an increasing trend in the middle and late periods of the study; QI9 showed an increasing trend in the early period.
Conclusions:This study demonstrated a trend toward continued improvement in quality of care in CPCs after certification. Accreditation should focus on the coordination and management of cross-cutting clinical issues, while not neglecting the accuracy of timely diagnosis.
Genome-wide association analysis and validation with KASP markers for nut and shell traits in almond (Prunus dulcis [Mill.] D.A.Webb).
Authors: Sideli Gina , Mather Diane , Wirthensohn Michelle , Dicenta Federico , Goonetilleke Shashi N , Martinez-Garcia Pedro Jose , Gradziel Thomas M 
Published: October 2022

Abstract
Almond shell hardness is an economically important quality trait that protects the integrity of the kernel. It is directly related with shelling or crack-out percent. In the U.S. and Australian markets, soft to semi-hard shells varieties have been used in the industry, while in the European market, hard shells are dominantly sold and distributed. The origin of a hard shell is from wild almond species. Soft shells in the U.S. and Australia have been selected for a higher crack-out percent or larger kernel and ease of processing. Here we examined breeding populations totaling 264 trees that were genotyped with genotyping-by-sequencing (GBS) where shell hardness was measured with a texture analyzer, and shell weight, in-shell weights, and shell thickness were measured and crack-out percent was calculated. In a genome-wide association seven genetic loci were found to be associated with shell hardness, crack-out percent, shell thickness and shell weight. Of these seven, one was shared between multiple traits. Kompetitive allele-specific PCR (KASP) assays were developed and tested in unrelated breeding lines from Australia, Spain, France and Italy. One KASP assay was found to be highly predictive of shell hardness in this diverse panel and can be applied for use in marker-assisted breeding.