bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antiviral effects of the petroleum ether extract of Tournefortia sibirica L. against enterovirus 71 infection in vitro and in vivo
Authors: Huang Xinyu , Li Jiemin , Hong Yan , Jiang Chenghan , Wu Jiaxin , Wu Min , Sheng Rui , Liu Hongtao , Sun Jie , Xin Ying , Su Weiheng 
Published: November 2022

Enterovirus 71 (EV71) is the major cause of severe hand, foot, and mouth disease (HFMD). Compared to other HFMD pathogens, like coxsackievirus A16 (CVA16), EV71 can invade the central nervous system and cause permanent damage. At present, there are no available antivirals against EV71 for clinical treatment. Herein, multiple Chinese botanical drugs were collected, and 47 types of botanical extracts were extracted using aqueous solutions and organic solvents. Based on the cytopathic effect inhibition assay, petroleum ether extract of Tournefortia sibirica L. (PE-TS) demonstrated 97.25% and 94.75% inhibition rates for EV71 infection (at 250 μg/ml) and CVA16 infection (at 125 μg/ml), respectively, with low cytotoxicity. Preliminary mechanistic studies showed that PE-TS inhibits replication of EV71 genomic RNA and synthesis of the EV71 protein. The released extracellular EV71 progeny virus titer decreased by 3.75 lg under PE-TS treatment. Furthermore, using a newborn mouse model, PE-TS treatment protected 70% and 66.7% of mice from lethal dose EV71 intracranial challenge via administration of intraperitoneal injection at 0.4 mg/g and direct lavage at 0.8 mg/g, respectively. The chemical constituents of the PE-TS were analyzed by Gas Chromatography-Mass Spectrometer (GC-MS), and a total of 60 compounds were identified. Compound-target network analysis and molecular docking implied potential bioactive compounds and their protein targets against EV71 associated pathology. The present study identified antiviral effects of PE-TS against EV71/CVA16 infection in vitro and EV71 infection in vivo, providing a potential antiviral botanical drug extract candidate for HFMD drug development.
Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice
Authors: Huang Mengying , Yu Lishuang , Wang Xiaoping , Wang Mingmin , Li Weili , Tang Jiayang , Ling Guanjing , Wei Xiaoqi , Wang Yong , Wang Wei , Wu Yan , Lu Linghui 
Published: November 2022

Transverse aortic constriction (TAC) is a frequently used model to investigate pressure overload-induced progressive heart failure (HF); however, there is considerable phenotypic variation among different mouse strains and even sub-strains. Moreover, less is known about the TAC model in ICR mice. Therefore, to determine the suitability of the ICR strain for TAC-induced HF research, we compared the effects of TAC on ICR and C57BL/6J mice at one, two and four weeks post-TAC via echocardiography, organ index, morphology, and histology. At the end of the study, behavior and gene expression patterns were assessed, and overall survival was monitored. Compared to the sham-operated mice, ICR and C57BL/6J mice displayed hypertrophic phenotypes with a significant increase in ventricle wall thickness, heart weight and ratio, and cross-sectional area of cardiomyocytes after a 2-week TAC exposure. In addition, ICR mice developed reduced systolic function and severe lung congestion 4 weeks post-TAC, whereas C57BL/6J did not. Besides, ICR mice demonstrated comparable survival, similar gene expression alteration but severer fibrotic remodeling and poor behavioral performance compared to the C57BL/6J mice. Our data demonstrated that ICR was quite sensitive to TAC-induced heart failure and can be an ideal research tool to investigate mechanisms and drug intervention for pressure overload-induced HF.
Study on potential markers for diagnosis of renal cell carcinoma by serum untargeted metabolomics based on UPLC-MS/MS
Authors: Wang Jun , Yang Wen-Yu , Li Xiao-Han , Xu Bei , Yang Yu-Wei , Zhang Bin , Dai Chun-Mei , Feng Jia-Fu 
Published: November 2022

Objective: Renal cell carcinoma (RCC) is the most common malignancy of the kidney. However, there is no reliable biomarker with high sensitivity and specificity for diagnosis and differential diagnosis. This study aims to analyze serum metabolite profile of patients with RCC and screen for potential diagnostic biomarkers.Methods: Forty-five healthy controls (HC), 40 patients with benign kidney tumor (BKT) and 46 patients with RCC were enrolled in this study. Serum metabolites were detected by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and then subjected to multivariate statistical analysis, metabolic pathway analysis and diagnostic performance evaluation.Results: The changes of glycerophospholipid metabolism, phosphatidylinositol signaling system, glycerolipid metabolism, d-glutamine and d-glutamate metabolism, galactose metabolism, and folate biosynthesis were observed in RCC group. Two hundred and forty differential metabolites were screened between RCC and HC groups, and 64 differential metabolites were screened between RCC and BKT groups. Among them, 4 differential metabolites, including 3-β-D-Galactosyl-sn-glycerol, 7,8-Dihydroneopterin, lysophosphatidylcholine (LPC) 19:2, and γ-Aminobutyryl-lysine (an amino acid metabolite), were of high clinical value not only in the diagnosis of RCC (RCC group vs. HC group; AUC = 0.990, 0.916, 0.909, and 0.962; Sensitivity = 97.73%, 97.73%, 93.18%, and 86.36%; Specificity = 100.00%, 73.33%, 80.00%, and 95.56%), but also in the differential diagnosis of benign and malignant kidney tumors (RCC group vs. BKT group; AUC = 0.989, 0.941, 0.845 and 0.981; Sensitivity = 93.33%, 93.33%, 77.27% and 93.33%; Specificity = 100.00%, 84.21%, 78.38% and 92.11%).Conclusion: The occurrence of RCC may involve changes in multiple metabolic pathways. The 3-β-D-Galactosyl-sn-glycerol, 7,8-Dihydroneopterin, LPC 19:2 and γ-Aminobutyryl-lysine may be potential biomarkers for the diagnosis or differential diagnosis of RCC.
CEOs’ leadership behaviors and new venture team stability: The effects of knowledge hiding and team collectivism
Authors: Ma Hongjia , Tang Sisi , Zhao Changyi 
Published: November 2022

PurposeThe reasons for new venture team instability gradually have become a vital issue in the entrepreneurship literature. While chief executive officers’ (CEOs) leadership behaviors is regarded as a critical element of governance within new venture teams, few studies explored the role played by CEOs’ leadership behaviors in new venture team stability. Drawing on the transactional-transformational leadership model, this study divides CEOs’ leadership behaviors in new ventures into two categories, namely, transformational and transactional leadership behaviors. Based on the social exchange theory and the social information processing theory, this study constructs a moderating mediation model to understand how transformational and transactional leadership affects new venture team stability. In this model, knowledge hiding is used as mediating role and team collectivism is used as moderating role.Design/methodology/approachThree-wave and two-source data was collected from 66 new ventures in China and an ordinary least squares hierarchical regression model and Hayes’ moderated-mediation approach were applied to test the hypotheses.Findings-The results show transformational leadership and transactional leadership are positively related to new venture team stability. Knowledge hiding mediates the association between transformational leadership and new venture team stability and that between transactional leadership and new venture team stability. Moreover, a high level of team collectivism corresponds to a stronger relationship between transformational leadership and knowledge hiding and a greater indirect effect of transformational leadership on new venture team stability through knowledge hiding.Originality/valueThis study explores the mechanisms and boundary conditions of the effect of transformational leadership, transactional leadership, and new venture team stability, which is an enrichment to the study of governance within new venture teams. It enlightens managers to take effective measures to reduce knowledge hiding and maintain team stability in new venture teams.
Validation of the Chinese version of the physical education teacher job satisfaction scale
Authors: Yuan Junfeng , Zhang Liping , Weng Shaojing , Yin Yujia , Li Chen , Luo Lin 
Published: November 2022

PurposeThe purpose of this study was to test the factor structure as well as the reliability of the Physical Education Teacher Job Satisfaction Scale (PETJSS).MethodThe scale’s structural validity, internal consistency and reliability were examined using CFA and Cronbach alpha. The predictive validity of the PETJSS was examined using Teacher Self-Efficacy (TSES-11) and the personal characteristics of the subjects.ResultThe three-factor structure of the PETJSS was confirmed. The PETJSS three-dimensional model had good internal consistency/reliability. The three dimensions of the PETJSS (colleague satisfaction, parent satisfaction and student behaviour satisfaction) explained 81.206% of the overall job satisfaction. Also, the PETJSS demonstrated the expected correlation with teachers’ self-efficacy, whilst the PETJSS test results were related to physical education teachers’ job titles.ConclusionThe PETJSS (Chinese version) can be considered as a valid and reliable method.
Emotion regulation, academic buoyancy, and academic adjustment of university students within a self-determination theory framework: A systematic review
Authors: Kritikou Marina , Giovazolias Theodoros 
Published: November 2022

IntroductionThe transition from secondary to tertiary education seems to be a stressful period for many students since they need to adjust to the new academic environment.MethodThis article is a systematic review of 4,285 articles. The aim of this review was to investigate the factors in the university environment associated with emotion regulation, academic buoyancy, and academic adjustment of tertiary students within a self-determination theory framework in combination with the nascent third wave of Positive Psychology. Forty-one articles met the inclusion criteria, all of which were rated as either good or moderate quality.ResultsThe bulk of the studies included in the systematic review reported individual factors, i.e., self-efficacy, intrinsic motivation, academic factors, i.e., intention to drop out, learning climate, and family and social factors i.e., faculty, peer, and parental autonomy support.DiscussionIn accordance with the third wave of Positive Psychology that focuses on how interpersonal and ecological factors create nurturing environments and positive institutions, the systematic review highlighted the factors that institutes should consider in order to help students adjust better to the academic environment.
Improving motor skills and psychosocial behaviors in children with autism spectrum disorder through an adapted judo program
Authors: Morales Jose , Pierantozzi Emanuela , Fukuda David H. , Garcia Vanessa , Guerra-Balic Miriam , Sevilla-Sánchez Marta , Carballeira Eduardo 
Published: November 2022

IntroductionThis study aimed to investigate the long-term effects of an adapted judo program on the motor skills and psychosocial abilities of children with Autism Spectrum Disorder (ASD).MethodsAll participants had been diagnosed with ASD and were assessed twice, one time at the start of the intervention and again 6 months later, with the Test of Gross Motor Development (TGMD-3) and the Gilliam Autism Rating Scale-Third Edition (GARS-3). A one-way repeated measures MANOVA was carried out in order to evaluate these assessments, and a mediation analysis was done to determine the relationship between them.ResultsThe experimental group significantly improved (p &lt; 0.05) from the pre-test to the post-test for several subtests of the TGMD-3 and the GARS-3.ConclusionThe study shows that participation in an adapted judo program clearly helps to improve the motor skills and psychosocial behaviors of children with ASD.
From contact coverage to effective coverage of community care for patients with severe mental disorders: A real-world investigation from Italy
Authors: Corrao Giovanni , Monzio Compagnoni Matteo , Barbato Angelo , D’Avanzo Barbara , Di Fiandra Teresa , Ferrara Lucia , Gaddini Andrea , Saponaro Alessio , Scondotto Salvatore , Tozzi Valeria D. , Carle Flavia , Carbone Simona , Chisholm Daniel H. , Lora Antonio 
Published: November 2022

ObjectivesTo measure the gap between contact and effective coverage of mental healthcare (MHC).Materials and methods45,761 newly referred cases of depression, schizophrenia, bipolar disorder, and personality disorder from four Italian regions were included. A variant of the self-controlled case series method was adopted to estimate the incidence rate ratio (IRR) for the relationship between exposure (i.e., use of different types of MHC such as pharmacotherapy, generic contact with the outpatient services, psychosocial intervention, and psychotherapy) and relapse (emergency hospital admissions for mental illness).Results11,500 relapses occurred. Relapse risk was reduced during periods covered by (i) psychotherapy for patients with depression (IRR 0.67; 95% CI: 0.49 to 0.91) and bipolar disorder (0.64; 0.29 to 0.99); (ii) psychosocial interventions for those with depression (0.74; 0.56 to 0.98), schizophrenia (0.83; 0.68 to 0.99), and bipolar disorder (0.55; 0.36 to 0.84), (iii) pharmacotherapy for patients with schizophrenia (0.58; 0.49 to 0.69), and bipolar disorder (0.59; 0.44 to 0.78). Coverage with generic care, in absence of psychosocial/psychotherapeutic interventions, did not affect risk of relapse.ConclusionThis study ascertained the gap between contact and effective coverage of MHC and showed that administrative data can usefully contribute to assess the effectiveness of a mental health system.
Mental health and life satisfaction in East and West Germany: Effects of generation and migration of citizens
Authors: Beutel Manfred E. , Braunheim Lisa , Heller Ayline , Schmutzer Gabriele , Decker Oliver , Brähler Elmar 
Published: November 2022

ObjectiveThe reunification of Germany after the separation between 1949 and 1990 has offered a unique chance of studying the impact of socialization, political transformation, and migration on mental health. The purpose of this article was to compare mental distress, resources, and life satisfaction (1) between residents of East and West Germany and migrants who have fled from East to West Germany before reunification and (2) between three generations.MethodsWe assessed anxiety, depression, resilience, self-esteem, and life satisfaction, comparing groups based on their residency and migration, as well as three different birth cohorts. Using a representative survey of 2006, analyses of variance show the differences between these groups. Based on a representative survey (N = 4,530), the effects of gender (53.6% women), residency/migration (74.4% grown up in the West, 20.4% in the East, 5.3% migrants from the East to the West) from three generations (32% born until 1945, 39% until 1967, and 29% to 1989), and positive and negative mental health indicators were analyzed.ResultsWomen reported higher distress and lower resilience. Residents of the Western states reported the lowest burden of distress (depressive and anxiety symptoms) and the highest overall life satisfaction, exceeding residents from the Eastern states and migrants from the Eastern to the Western states. Migrants from the Eastern to the Western states, however, reported the lowest resilience and self-esteem. They reported lower satisfaction with income, living conditions (compared to the Western residents), and the lowest levels of satisfaction with family (compared to East and West).ConclusionOverall, our data point to inequalities between the Eastern and Western states regarding mental health 16 years after reunification favoring the residents of the Western states by lower distress and life satisfaction. Our data attest to the stresses and adjustments associated with migration from the Eastern to the Western states before reunification. A lower level of mental health and life satisfaction in the oldest generation may be related to the sequelae of World War II and also to aging.
A capillary flow-driven microfluidic device for point-of-care blood plasma separation
Authors: Brakewood William , Lee Kiara , Schneider Lindsay , Lawandy Nabil , Tripathi Anubhav 
Published: November 2022

Plasma has significant utility as an input for diagnostics and screening for conditions such as viral infections, cancer, and more. However, plasma is difficult to obtain at the point-of-care, as separation from whole blood is typically carried out via centrifugation. We have designed and optimized a low-cost, simple-to-operate microfluidic device which carries out the separation of plasma from whole blood. The device utilizes depth filtration as its separation mechanism and collects plasma via capillary action, allowing for operation without components that drive flow externally. We first optimized device dimensions and operating parameters and demonstrated consistent separation efficiencies for the samples with hematocrits ranging from 25–65%. The impact of input sample hematocrit percentage on flow rate through the device was also examined, with samples with hematocrits greater than 45% decreasing plasma flow rate. Lastly, we evaluated the ability of this device to produce plasma with a high protein concentration and found no significant difference between protein levels in samples from the device compared to samples produced via centrifugation. This system produced plasma with a maximum separation efficiency of 88.5% and achieved a maximum plasma volume of ∼14 μl from a 50 μl whole blood input. The low cost, simplicity of operation, and high plasma quality associated with this device give it many advantages in a point-of-care setting. This device could be integrated into plasma-based diagnostic workflows to increase access to various types of disease testing and monitoring.
On the importance of neutral composition and temperature measurements in the 100–200 km altitude region
Authors: Jones McArthur , Emmert John T. , Gan Quan , Yue Jia 
Published: November 2022

Currently, thermospheric species densities and temperatures between ∼100 and 200 km are not known to the accuracy needed to fully characterize how the thermosphere transitions from a well-mixed atmosphere to a diffusively separated atmosphere with zero temperature gradient. This greatly inhibits scientific discovery attainable from either models or observations in this region, especially the understanding of mechanisms that drive thermosphere and ionospheric variability from space weather to climatological time scales. The purpose of this paper is to highlight the importance and critical need for new, global, height-resolved neutral composition (O, O2, N2) and temperature measurements in the new ignorosphere: the 100–200 km region of the thermosphere. We conclude with observation recommendations and requirements for new comprehensive composition and temperature measurements in the 100–200 km altitude region that would lead to significant advances in thermosphere-ionosphere science, space weather, and space climate.
Transcriptome Level Reveals the Triterpenoid Saponin Biosynthesis Pathway of Bupleurum falcatum L.
Authors: Li Yuchan , Zhao Jun , Chen Hua , Mao Yanping , Yang Yuping , Feng Liang , Mo Chuanxin , Huang Lin , Hou Dabin , Yu Ma 
Published: November 2022

Bupleurum falcatum L. is frequently used in traditional herbal medicine in Asia. Saikosaponins (SSs) are the main bioactive ingredients of B. falcatum, but the biosynthetic pathway of SSs is unclear, and the biosynthesis of species-specific phytometabolites is little known. Here we resolved the transcriptome profiles of B. falcatum to identify candidate genes that might be involved in the biosynthesis of SSs. By isoform sequencing (Iso-Seq) analyses of the whole plant, a total of 26.98 Gb of nucleotides were obtained and 124,188 unigenes were identified, and 81,594 unigenes were successfully annotated. A total of 1033 unigenes of 20 families related to the mevalonate (MVA) pathway and methylerythritol phosphate (MEP) pathway of the SS biosynthetic pathway were identified. The WGCNA (weighted gene co-expression network analysis) of these unigenes revealed that only the co-expression module of MEmagenta, which contained 343 unigenes, was highly correlated with the biosynthesis of SSs. Comparing differentially expressed gene analysis and the WGCNA indicated that 130 out of 343 genes of the MEmagenta module exhibited differential expression levels, and genes with the most “hubness” within this module were predicted. Manipulation of these genes might improve the biosynthesis of SSs.
Four New Species of Macquartia (Diptera: Oestroidea) from China and Phylogenetic Implications of Tachinidae
Authors: Li Henan , Zhang Baihui , Pei Wenya , Sun Haoran , Chen Jinliang , Gao Xinzhang , Peng Honglin , Zhang Dong , Zhang Chuntian 
Published: November 2022

Macquartia Robineau-Desvoidy (Diptera: Tachinidae, Tachininae) represents one of the most ancient evolutionary lineages of tachinids, parasitizing Chrysomelidae larvae. We found four new Macquartia species collected by malaise traps, namely M. brunneisquama sp. nov., M. chinensis sp. nov., M. flavifemorata sp. nov., and M. flavipedicel sp. nov. These new species are described and illustrated, and their comparison with congeners as well as an identification key to the 12 species of Macquartia from China known to date are included. To determine the significance of the mitogenome architecture and evolution across different tachinid lineages of this primitive taxonomic group, four complete mitochondrial genomes were sequenced, annotated, and analyzed. The gene arrangements are consistent with the ancestral insect mitogenomes. The full-length sequences and protein-coding genes (PCGs) of the mitogenomes of the four species are all AT-biased. Analyses of Ka/Ks and overall p-genetic distance demonstrated that nad5 showed the highest evolutionary rate and nad1/nad4L were the most conserved genes among the four species. Phylogenetic reconstruction based on 13 PCGs strongly supported the monophyly of Macquartia, and the relationships of the four species are (M. flavifemorata + (M. flavipedicel + (M. brunneisquama + M. chinensis))). This study will help enhance our understanding of the taxonomic status and phylogenetic relationships in Tachinidae.