bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of an ultra-wideband omnidirectional and polarization insensitive flower petal antenna for potential ambient electromagnetic energy harvesting applications
Authors: Wang Wei-Chih , Garu Prabir 
Published: April 2022

AbstractDeveloping a polarization insensitive, omnidirectional, and ultra-wideband (UWB) antenna is highly desired for improving the utilization of freely available electromagnetic (EM) radiation energy. In this study, we have designed an UWB antenna based on tapered flower petals and numerically analyzed to show that it is a promising candidate for energy harvesting applications in the infrared (IR) to UV–visible regime. The impacts of design strategy and parameters on the absorption performance are studied numerically. The antenna shows a high performance in both bandwidth and absorptivity (average absorption of 84.5% spanning a broad range from 25 to 800 THz) under normal incidence of plane waves. To get a better understanding behind such high and UWB absorption mechanism, we investigated the electric field (E-field) distribution over the structure. The antenna also generates less than 5% absorption deviation between normal to 45° incident angle and 0.05% absorption deviation between 0° and 90° polarizations for both transverse electric (TE) and transverse magnetic (TM) modes. This new design aspect and the numerical findings unfolds the new direction for numerous EM wideband applications such as THz technology, photo detection, bolometric sensing, camouflaging, spectral imaging, and ambient EM energy harvesting applications.
PET imaging of mitochondrial function in acute doxorubicin-induced cardiotoxicity: a proof-of-principle study
Authors: Detmer Felicitas J. , Alpert Nathaniel M. , Moon Sung-Hyun , Dhaynaut Maeva , Guerrero J. Luis , Guehl Nicolas J. , Xing Fangxu , Brugarolas Pedro , Shoup Timothy M. , Normandin Marc D. , Pelletier-Galarneau Matthieu , El Fakhri Georges , Petibon Yoann 
Published: April 2022

AbstractMitochondrial dysfunction plays a key role in doxorubicin-induced cardiotoxicity (DIC). In this proof-of-principle study, we investigated whether PET mapping of cardiac membrane potential, an indicator of mitochondrial function, could detect an acute cardiotoxic effect of doxorubicin (DOX) in a large animal model. Eight Yucatan pigs were imaged dynamically with [18F](4-Fluorophenyl)triphenylphosphonium ([18F]FTPP+) PET/CT. Our experimental protocol included a control saline infusion into the left anterior descending coronary artery (LAD) followed by a DOX test infusion of either 1 mg/kg or 2 mg/kg during PET. We measured the change in total cardiac membrane potential (ΔΨT), a proxy for the mitochondrial membrane potential, ΔΨm, after the saline and DOX infusions. We observed a partial depolarization of the mitochondria following the DOX infusions, which occurred only in myocardial areas distal to the intracoronary catheter, thereby demonstrating a direct association between the exposure of the mitochondria to DOX and a change in ΔΨT. Furthermore, doubling the DOX dose caused a more severe depolarization of myocardium in the LAD territory distal to the infusion catheter. In conclusion, [18F]FTPP+ PET-based ΔΨT mapping can measure partial depolarization of myocardial mitochondria following intracoronary DOX infusion in a large animal model.
Early menopause is associated with increased risk of retinal vascular occlusions: a nationwide cohort study
Authors: Hwang Sungsoon , Kang Se Woong , Choi Kyung Jun , Son Ki Young , Lim Dong Hui , Shin Dong Wook , Choi DooSeok , Kim Sang Jin 
Published: April 2022

AbstractThis nationwide population-based cohort study evaluated the association between female reproductive factors and the incidence of retinal vein occlusion (RVO) and retinal artery occlusion (RAO) using data provided by the Korea National Health Insurance Service. A total of 2,289,347 postmenopausal women over 50 years of age who participated in both national health screening and cancer screening in 2013 or 2014 were included. Data on female reproductive factors, including age at menarche, age at menopause, parity, history of hormone replacement therapy, and oral contraceptive pill usage, were collected. Patients were followed up until December 2018, and incident cases of RVO and RAO were identified using registered diagnostic codes from claim data. During an average follow-up period of 4.90 years, 7461 and 1603 patients were newly diagnosed with RVO and RAO, respectively. In the multivariable-adjusted Cox proportional hazard model, patients who experienced menopause after 55 years of age had a lower risk of RVO and RAO development compared to those who had menopause before 45 years of age, with a hazard ratio (95% confidence interval) of 0.83 (0.76–0.95) for RVO and 0.80 (0.66‒0.98) for RAO. In conclusion, early menopause was an independent risk factor for future development of RVO and RAO.
Direct effects of elevated dissolved CO2 can alter the life history of freshwater zooplankton
Authors: Ramaekers Lana , Pinceel Tom , Brendonck Luc , Vanschoenwinkel Bram 
Published: April 2022

AbstractDissolved CO2 levels (pCO2) are increasing in lentic freshwaters across the globe. Recent studies have shown that this will impact the nutritional quality of phytoplankton as primary producers. However, the extent to which freshwater zooplankton may also be directly affected remains unclear. We test this in three model species representative of the main functional groups of primary consumers in freshwaters; the water flea Daphnia magna, the seed shrimp Heterocypris incongruens and the rotifer Brachionus calyciflorus. We experimentally exposed individuals to three pCO2 levels (1,500; 25,500 and 83,000 ppm) to monitor changes in life history in response to current, elevated and extreme future pCO2 conditions in ponds and shallow lakes. All species had reduced survival under the extreme pCO2 treatment, but the water flea was most sensitive. Body size and reproduction were reduced at 25,500 ppm in the water flea and the seed shrimp and population growth was delayed in the rotifer. Overall, our results show that direct effects of pCO2 could impact the population dynamics of freshwater zooplankton. By differentially modulating the life history of functional groups of primary consumers, elevated pCO2 has the potential to change the evolutionary trajectories of populations as well as the ecological functioning of freshwater communities.
OCT1-target neural gene PFN2 promotes tumor growth in androgen receptor-negative prostate cancer
Authors: Obinata Daisuke , Funakoshi Daigo , Takayama Kenichi , Hara Makoto , Niranjan Birunthi , Teng Linda , Lawrence Mitchell G. , Taylor Renea A. , Risbridger Gail P. , Suzuki Yutaka , Takahashi Satoru , Inoue Satoshi 
Published: April 2022

AbstractAndrogen and androgen receptor (AR) targeted therapies are the main treatment for most prostate cancer (PC) patients. Although AR signaling inhibitors are effective, tumors can evade this treatment by transforming to an AR-negative PC via lineage plasticity. OCT1 is a transcription factor interacting with the AR to enhance signaling pathways involved in PC progression, but its role in the emergence of the AR-negative PC is unknown. We performed chromatin immunoprecipitation sequencing (ChIP-seq) in patient-derived castration-resistant AR-negative PC cells to identify genes that are regulated by OCT1. Interestingly, a group of genes associated with neural precursor cell proliferation was significantly enriched. Then, we focused on neural genes STNB1 and PFN2 as OCT1-targets among them. Immunohistochemistry revealed that both STNB1 and PFN2 are highly expressed in human AR-negative PC tissues. Knockdown of SNTB1 and PFN2 by siRNAs significantly inhibited migration of AR-negative PC cells. Notably, knockdown of PFN2 showed a marked inhibitory effect on tumor growth in vivo. Thus, we identified OCT1-target genes in AR-negative PC using a patient-derived model, clinicopathologial analysis and an animal model.
Upper limb isokinetic muscle strength predicts the performance in cross-country sit-skiing
Authors: Liu Chenglin , Tian Yuan , Zhou Longfeng , Tian Zhulin , Sun Gang , Yin Jun , Zhou Zhixiong 
Published: April 2022

AbstractThe double poling (DP) technique in cross-country sit-skiing is primarily considered as an upper-body exercise. The upper limb muscle strength and motion economy are important factors accounting for DP performance in cross-country sit-skiing. The present study investigates how upper limb muscle strength predicts DP performance in cross-country sit-skiing. A total of 19 female non-disabled college students (age 23.2 ± 0.8 years, BMI 20.4 ± 2.2) performed 30-s and 3-min DP performance tests using a sit-skiing ergometer. Isokinetic muscle strength of the shoulder and elbow extensor were measured at the angular velocity of 30°/s, 60°/s, and 120°/s with an ISOMED2000 isokinetic system. A medium correlation was found between DP output power and isokinetic upper limb muscle strength (shoulder strength at all speeds, r = 0.39–0.74, p ≤ 0.1). Multiple regression analyses which were employed to predict power production in the 30-s and 3-min tests showed that shoulder extension strength at 60°/s accounted for 34% of the variation in the 30-s test, and 40% of the variance in the 3-min test. Muscle strength and biomechanical analysis of DP process indicated that upper limb extensor muscle strength and muscle coordination were important factors for the power output generation in sit-skiing DP. These results may use to guide special physical fitness training for paralympic cross-country sit-skiing.
An influence of dew point temperature on the occurrence of Mycobacterium tuberculosis disease in Chennai, India
Authors: Krishnan Rajendran , Thiruvengadam Kannan , Jayabal Lavanya , Selvaraju Sriram , Watson Basilea , Malaisamy Muniyandi , Nagarajan Karikalan , Tripathy Srikanth P. , Chinnaiyan Ponnuraja , Chandrasekaran Padmapriyadarsini 
Published: April 2022

AbstractClimate factors such as dew point temperature, relative humidity and atmospheric temperature may be crucial for the spread of tuberculosis. This study was conducted for the first time to investigate the relationship of climatic factors with TB occurrence in an Indian setting. Daily tuberculosis notification data during 2008–2015 were generated from the National Treatment Elimination Program, and analogous daily climatic data were obtained from the Regional Meteorological Centre at Chennai city, Tamil Nadu, India. The decomposition method was adopted to split the series into deterministic and non-deterministic components, such as seasonal, non-seasonal, trend and cyclical, and non-deterministic climate factors. A generalized linear model was used to assess the relation independently. TB disease progression from latent stage infection to active was supported by higher dew point temperature and moderate temperature. It had a significant association with TB progression in the summer and monsoon seasons. The relative humidity may be favored in the winter and post-monsoon. The water tiny dew droplets may support the TB bacterium to recuperate in the environment.
Evaluation of dental caries detection with quantitative light-induced fluorescence in comparison to different field of view devices
Authors: Oh Song Hee , Choi Jin-Young , Kim Seong-Hun 
Published: April 2022

AbstractThis study evaluated dental caries detection ability between the Qraycam and Qraypen on the same dental caries lesions. A total of 178teeth from 61patients were imaged using Qraypen C®(QC) and Qraycam Pro®(QP) devices and evaluated using analysis software (QA2). Occlusal, secondary, and proximal dental caries were evaluated and scored according to International Caries Detection and Assessment System(ICDAS II) and X-ray criteria. Bland–Altman plots were used to compare quantitative light-induced fluorescence(QLF) parameters obtained from the different QLF devices. Sensitivity, specificity, and area under the receiver operating characteristic curve(AUROC) were calculated. The ΔFaver. of the QLF-parameters showed that the mean difference between the two different QLF devices was close to zero and that the ± 5 error value was included in the mean ± 1.96SD range for the detection of dental caries. The accuracies for diagnosing occlusal dental caries were 0.83–0.96 and 0.81–0.82 and the accuracies for diagnosing proximal dental caries were 0.52–0.62 and 0.52–0.71 for the QC and QP devices, respectively. In conclusion, the ΔFaver. obtained from the QP showed diagnostic value mainly for screening of demineralized teeth. For teeth selected through screening, the depth of the lesion must be precisely evaluated using additional QP and radiographic imaging.
Separation of U87 glioblastoma cell-derived small and medium extracellular vesicles using elasto-inertial flow focusing (a spiral channel)
Authors: Shiri Farhad , Feng Haidong , Petersen Kevin E. , Sant Himanshu , Bardi Gina T. , Schroeder Luke A. , Merchant Michael L. , Gale Bruce K. , Hood Joshua L. 
Published: April 2022

AbstractNanoscale and microscale cell-derived extracellular vesicle types and subtypes are of significant interest to researchers in biology and medicine. Extracellular vesicles (EVs) have diagnostic and therapeutic potential in terms of biomarker and nanomedicine applications. To enable such applications, EVs must be isolated from biological fluids or separated from other EV types. Developing methods to fractionate EVs is of great importance to EV researchers. Our goal was to begin to develop a device that would separate medium EVs (mEVs, traditionally termed microvesicles or shedding vesicles) and small EVs (sEVs, traditionally termed exosomes) by elasto-inertial effect. We sought to develop a miniaturized technology that works similar to and provides the benefits of differential ultracentrifugation but is more suitable for EV-based microfluidic applications. The aim of this study was to determine whether we could use elasto-inertial focusing to re-isolate and recover U87 mEVs and sEVs from a mixture of mEVs and sEVs isolated initially by one round of differential ultracentrifugation. The studied spiral channel device can continuously process 5 ml of sample fluid per hour. Using the channel, sEVs and mEVs were recovered and re-isolated from a mixture of U87 glioma cell-derived mEVs and sEVs pre-isolated by one round of differential ultracentrifugation. Following two passes through the spiral channel, approximately 55% of sEVs were recovered with 6% contamination by mEVs (the recovered sEVs contained 6% of the total mEVs). In contrast, recovery of U87 mEVs and sEVs re-isolated using a typical second centrifugation wash step was only 8% and 53%, respectively. The spiral channel also performed similar to differential ultracentrifugation in reisolating sEVs while significantly improving mEV reisolation from a mixture of U87 sEVs and mEVs. Ultimately this technology can also be coupled to other microfluidic EV isolation methods in series and/or parallel to improve isolation and minimize loss of EV subtypes.
Learned self-regulation in top-level managers through neurobiofeedback training improves decision making under stress
Authors: Iodice Pierpaolo , Cannito L. , Chaigneau A. , Palumbo R. 
Published: April 2022

AbstractTop-level management teams are particularly exposed to stress factors as they frequently have to make important decision under stress. While an existing body of research evidence suggests that stress negatively affects decision-making processes, very little is known about possible strategies to reduce these negative effects. The aim of the current work is to investigate the effect of training self-regulation ability through neurobiofeedback on managers’ intertemporal and risky decision making. Twenty-three managers were assigned to the experimental or the control condition. All participants performed, two decisional tasks, before and after a training phase. The tasks were administered through mouse tracker software, in order to measure participants’ delay discounting and risk taking propensity on both explicit and implicit choice parameters. During the training phase, the experimental condition received a training protocol based on stress assessment tests via neurobiofeedback signals (i.e., temperature and skin conductance), with the goal of improving self-regulation ability while the control condition was administered a control training. The main result of this study is to have conclusively demonstrated that NBF training increases an individual's ability to self-regulate stress-related psychophysiological phenomena. Consequently, the improved ability to manage one's own reaction to stress enables a reduction in instinctive behavior during a probabilistic choice task.
Telomere length was associated with grade and pathological features of meningioma
Authors: Yamakawa Keiko , Mukai Yuri , Ye Juanjuan , Muto-Ishizuka Mariko , Ito Masumi , Tanimoto Misa , Suizu Futoshi , Asano Kenichiro , Kurose Akira , Matsuda Yoko 
Published: April 2022

AbstractTelomeres are tandem repeats of the TTAGGG sequence at chromosomal ends and afford protection against chromosomal instability. To investigate the contribution of telomere dysfunction in meningiomas, here we estimate the associations between telomere length, tumor grade, and proliferation index in a series of 14 archived samples, using quantitative-fluorescence in situ hybridization, Ki67 immunostaining, and pathological analysis. The number of mitoses per 10 high-power fields (HPF) and Ki67 index was higher in grade III cases than in grade I or grade II cases. Telomere length was negatively associated with both the number of mitoses/10HPF and Ki67 index. Meningioma cases with atypical mitosis, a morphological marker of chromosomal instability, exhibited shortened telomeres. Among telomere-shortened meningioma cases, 40% were grade I, 20% were grade II, and 100% were grade III. In grade I or II meningiomas, shortened telomeres lacked high proliferation activity and atypical mitosis. In conclusion, telomere shortening might be pivotal in the development of high-grade meningioma. Analysis of telomere length might be a selective marker for meningiomas with high-grade malignant potential.
Experimental evidence of auxeticity in ion implanted single crystal calcite
Authors: Liao Michael E. , Li Chao , Shah Nachiket , Hsiao Yi-Hsuan , Bauchy Mathieu , Sant Gaurav , Goorsky Mark S. 
Published: April 2022

AbstractWe report initial experimental evidence of auxeticity in calcite by ion implanting (1010) oriented single crystalline calcite with Ar+ at room temperature using an ion energy of 400 keV and a dose of 1 × 1014 cm−2. Lattice compression normal to the substrate surface was observed, which is an atypical result for ion implanted materials. The auxetic behavior is consistent with predictions that indicate auxeticity had been predicted along two crystallographic directions including [1010]. Materials with a positive Poisson’s ratio experience lattice expansion normal to the substrate surface when ion implanted, whereas lattice contraction normal to the surface is evidence of auxetic behavior. Triple-axis X-ray diffraction measurements confirmed the auxetic strain state of the implanted calcite substrates. Reciprocal space maps for the symmetric 3030 and asymmetric 1450 reflections revealed that the implanted region was fully strained (pseudomorphic) to the bulk of the substrate, as is typical with implanted single crystals. A symmetric (3030) ω:2θ line scan was used with X-ray dynamical diffraction simulations to model the strain profile and extract the variation of compressive strain as a function of depth normal to the substrate surface. SRIM calculations were performed to obtain a displacement-per-atom profile and implanted Ar+ concentration profile. It was found that the strain profile matches the displacement-per-atom profile. This study demonstrated the use of ion implantation and X-ray diffraction methods to probe mechanical properties of materials and to test predictions such as the auxeticity.
Bovine host genome acts on rumen microbiome function linked to methane emissions
Authors: Martínez-Álvaro Marina , Auffret Marc D. , Duthie Carol-Anne , Dewhurst Richard J. , Cleveland Matthew A. , Watson Mick , Roehe Rainer 
Published: April 2022

AbstractOur study provides substantial evidence that the host genome affects the comprehensive function of the microbiome in the rumen of bovines. Of 1,107/225/1,141 rumen microbial genera/metagenome assembled uncultured genomes (RUGs)/genes identified from whole metagenomics sequencing, 194/14/337 had significant host genomic effects (heritabilities ranging from 0.13 to 0.61), revealing that substantial variation of the microbiome is under host genomic control. We found 29/22/115 microbial genera/RUGs/genes host-genomically correlated (|0.59| to |0.93|) with emissions of the potent greenhouse gas methane (CH4), highlighting the strength of a common host genomic control of specific microbial processes and CH4. Only one of these microbial genes was directly involved in methanogenesis (cofG), whereas others were involved in providing substrates for archaea (e.g.bcdandpccB), important microbial interspecies communication mechanisms (ABC.PE.P), host-microbiome interaction (TSTA3) and genetic information processes (RP-L35). In our population, selection based on abundances of the 30 most informative microbial genes provided a mitigation potential of 17% of mean CH4emissions per generation, which is higher than for selection based on measured CH4using respiration chambers (13%), indicating the high potential of microbiome-driven breeding to cumulatively reduce CH4emissions and mitigate climate change.
SimiC enables the inference of complex gene regulatory dynamics across cell phenotypes
Authors: Peng Jianhao , Serrano Guillermo , Traniello Ian M. , Calleja-Cervantes Maria E. , Chembazhi Ullas V. , Bangru Sushant , Ezponda Teresa , Rodriguez-Madoz Juan Roberto , Kalsotra Auinash , Prosper Felipe , Ochoa Idoia , Hernaez Mikel 
Published: April 2022

AbstractSingle-cell RNA-Sequencing has the potential to provide deep biological insights by revealing complex regulatory interactions across diverse cell phenotypes at single-cell resolution. However, current single-cell gene regulatory network inference methods produce a single regulatory network per input dataset, limiting their capability to uncover complex regulatory relationships across related cell phenotypes. We present SimiC, a single-cell gene regulatory inference framework that overcomes this limitation by jointly inferring distinct, but related, gene regulatory dynamics per phenotype. We show that SimiC uncovers key regulatory dynamics missed by previously proposed methods across a range of systems, both model and non-model alike. In particular, SimiC was able to uncover CAR T cell dynamics after tumor recognition and key regulatory patterns on a regenerating liver, and was able to implicate glial cells in the generation of distinct behavioral states in honeybees. SimiC hence establishes a new approach to quantitating regulatory architectures between distinct cellular phenotypes, with far-reaching implications for systems biology.