bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional analysis of ARF1 from Cymbidium goeringii in IAA response during leaf development
Authors: Xu Zihan , Li Fangle , Li Meng , He Yuanhao , Chen Yue , Hu Fengrong 
Published: March 2022

Background
Cymbidium is an economically important genus of flowering orchids cultivated in China because of showing graceful leaf shapes and elegant flower coloration. However, the deterioration of the ecological environment and the difficulty of conservation management have become increasing challenges for maintaining its germplasm resources. ARFs are critical transcription factors in the auxin signaling pathway and have been found to play pivotal roles in leaf growth and development in previous studies. However, their functions and mechanisms in Cymbidium goeringii remain to be clarified.

Methods
The sequence of the CgARF1 gene was analyzed by bioinformatics. The expression of this gene in different tissues and under IAA treatment was detected by quantitative real-time PCR analysis. The CgARF1 gene was overexpressed in wild-type Arabidopsis and Nicotiana benthamiana via the Agrobacterium infection method. Acetone-ethanol solvent extraction was applied for the determination of chlorophyll contents, and the contents of endogenous hormones were determined using the enzyme-linked immunosorbent assay technique.

Results
CgARF1 cloned from C. goeringii ‘Songmei’ was 2,049 bp in length and encoded 682 amino acids containing three typical domains: a B3 DNA binding domain, an Aux_resp domain and an AUX/IXX family domain. The expression pattern of CgARF1 in different tissues of C. goeringii showed that its expression was highest in the leaves and changed greatly under IAA treatment. Subcellular localization studies showed that the protein was mainly localized in the cell nucleus. CgARF1-overexpressing lines exhibited leaf senescence and a decreased chlorophyll content. Under IAA treatment, CgARF1 regulates the rooting length, rooting number and rooting rate from detached leaves. The levels of endogenous hormones in transgenic leaves were also significantly changed.

Conclusion
These results indicated that CgARF1 overexpression is responsive to IAA treatment during leaf development. This study provides a foundation for future research on the function of the ARF gene family in C. goeringii.
Feature screening for survival trait with application to TCGA high-dimensional genomic data
Authors: Wang Jie-Huei , Li Cai-Rong , Hou Po-Lin 
Published: March 2022

Background
In high-dimensional survival genomic data, identifying cancer-related genes is a challenging and important subject in the field of bioinformatics. In recent years, many feature screening approaches for survival outcomes with high-dimensional survival genomic data have been developed; however, few studies have systematically compared these methods. The primary purpose of this article is to conduct a series of simulation studies for systematic comparison; the second purpose of this article is to use these feature screening methods to further establish a more accurate prediction model for patient survival based on the survival genomic datasets of The Cancer Genome Atlas (TCGA).

Results
Simulation studies prove that network-adjusted feature screening measurement performs well and outperforms existing popular univariate independent feature screening methods. In the application of real data, we show that the proposed network-adjusted feature screening approach leads to more accurate survival prediction than alternative methods that do not account for gene-gene dependency information. We also use TCGA clinical survival genetic data to identify biomarkers associated with clinical survival outcomes in patients with various cancers including esophageal, pancreatic, head and neck squamous cell, lung, and breast invasive carcinomas.

Conclusions
These applications reveal advantages of the new proposed network-adjusted feature selection method over alternative methods that do not consider gene-gene dependency information. We also identify cancer-related genes that are almost detected in the literature. As a result, the network-based screening method is reliable and credible.
Observational study of medical marijuana as a treatment for treatment‐resistant epilepsies
Authors: Devinsky Orrin , Marmanillo Angelica , Hamlin Theresa , Wilken Philip , Ryan Daniel , Anderson Conor , Friedman Daniel , Todd George 
Published: March 2022

AbstractObjectivesMedical cannabis formulations with cannabidiol (CBD) and delta‐9‐tetrahydrocannabinol (THC) are widely used to treat epilepsy. We studied the safety and efficacy of two formulations.MethodsWe prospectively observed 29 subjects (12 to 46 years old) with treatment‐resistant epilepsies (11 Lennox–Gastaut syndrome; 15 with focal or multifocal epilepsy; three generalized epilepsy) were treated with medical cannabis (1THC:20CBD and/or 1THC:50CBD; maximum of 6 mg THC/day) for ≥24 weeks. The primary outcome was change in convulsive seizure frequency from the pre‐treatment baseline to the stable optimal dose phase.ResultsThere were no significant differences during treatment on stable maximal doses for convulsive seizure frequency, seizure duration, postictal duration, or use of rescue medications compared to baseline. No benefits were seen for behavioral disorders or sleep duration; there was a trend for more frequent bowel movements compared to baseline. Ten adverse events occurred in 6/29 patients, all were transient and most unrelated to study medication. No serious adverse events were related to study medication.InterpretationOur prospective observational study of two high‐CBD/low‐THC formulations found no evidence of efficacy in reducing seizures, seizure duration, postictal duration, or rescue medication use. Behavioral disorders or sleep duration was unchanged. Study medication was generally well tolerated. The doses of CBD used were lower than prior studies. Randomized trials with larger cohorts are needed, but we found no evidence of efficacy for two CBD:THC products in treating epilepsy, sleep, or behavior in our population.
Membranotronics: Bioinspired Nonlinear Ion Transport with Negative Differential Resistance Based on Elastomeric Membrane System
Authors: Faghih Maryam , Karnaushenko Daniil , Besford Quinn A. , Becker Christian , Ravishankar Rachappa , Karnaushenko Dmitriy D. , Cuniberti Gianaurelio , Fery Andreas , Schmidt Oliver G. 
Published: March 2022

AbstractBiological neural networks enable rapid communication between cells, which has inspired the development of artificial smart neuro‐mimetic systems. In this work, freestanding elastomeric membranes are fabricated with 3D structured holes to induce nonlinear ion‐based transport. These 3D microholes are embedded in a soft polymeric membrane via a grayscale lithographical process and demonstrate ionic rectification, negative differential ionic resistance, ionic conductivity gating, and spiking under an applied electric field bias. The ion transport behavior of these membranes is affected by the shape, size, number, and chemical functionalization of the embedded microholes. The microholes are functionalized with responsive poly(N‐isopropylacrylamide) polymer brush layers allowing to alter the electric gating behavior by external stimuli that includes temperature and chemical composition of the surrounding environment. The electrically biased membranes with a linear array of eight equidistantly spaced microholes exhibit synchronous gating of ionic transport through each one. These individual microholes are equipped with circumjacent blocking stretchable electrodes to simultaneously measure the exact onset time delay of the spikes during the synchronous gating. The operation of the freestanding elastomeric membranes with embedded microholes mimics the behavior of neural membranes. Membranotronics will pave the way toward bioinspired soft artificial neuro‐mimetic systems mechanically and electrochemically comparable to their biological counterpart.
Barycenter Self‐Adapting Triboelectric Nanogenerator for Sea Water Wave High‐Entropy Energy Harvesting and Self‐Powered Forecasting in Marine Meteorology
Authors: Yang Yuhan , Wen Jing , Chen Furong , Hao Yutao , Gao Xiaobo , Jiang Tao , Chen Baodong , Wang Zhong Lin 
Published: March 2022

AbstractAs a high entropy energy (HEE), irregular seawater waves are a promising source of sustainable energy, and the development of matched harvesting technology is imperative to accommodate the new era. Here, a barycenter self‐adapting triboelectric nanogenerator (BSA‐TENG) that addresses these concerns and shows its applications as a distributed power source in isolated waters is reported. Benefitting from the physical gravity‐guided structure design, random vibrational water wave HEE can be effectively converted into rotational mechanical energy and then converted into electric energy. In a nonlinear seawater wave pool, a unit delivers a peak power of 0.1 mW under a load resistance of 500 MΩ when the working frequency is <1 Hz. On this basis, a self‐powered temperature sensor system and wireless signal transmitter have been developed and driven entirely by the BSA‐TENG. This work plays a key role in promoting ocean HEE harvesting and provides a workable way for a self‐powered service system in seawater wave and marine meteorology monitoring and forecasting.
Multi‐Mode Antibacterial Strategies Enabled by Gene‐Transfection and Immunomodulatory Nanoparticles in 3D‐Printed Scaffolds for Synergistic Exogenous and Endogenous Treatment of Infections
Authors: Qu Xinhua , Wang Minqi , Wang Miaochen , Tang Haozheng , Zhang Shutao , Yang Hongtao , Yuan Weien , Wang You , Yang Jianping , Yue Bing 
Published: March 2022

AbstractAs research on refractory Staphylococcus aureus‐related implant infection intensifies, certain challenges remain, including low antibiotic concentrations within infected areas, immune escape achieved by intracellular bacteria, myeloid‐derived suppressor cells (MDSCs) inducing regional immunosuppression, and recurrence of residual pathogenic bacteria after drug suspension. Herein, a novel antimicrobial system to simultaneously address these issues is proposed. Specifically, an oxygen‐species‐responsive 3D‐printed scaffold with shell–core nanoparticles is designed, which are loaded with an antimicrobial peptide plasmid (LL37 plasmid) and have LL37 grafted on their surface (LL37@ZIF8‐LL37). The surface‐grafted LL37 directly kills S. aureus and, following entry into cells, the nanoparticles kill intracellular bacteria. Moreover, in vitro and in vivo, following translation of the LL37 plasmid, cells function as factories of the antimicrobial peptide, thereby generating a continuous, prolonged antibacterial effect at the site of infection. This system significantly reduces the abnormal increase in MDSCs within the infected microenvironment, thus relieving the immunosuppressive state and restoring a protective antimicrobial immune response. Hence, this proposed antimicrobial system provides an antimicrobial immune response and a novel strategy for S. aureus‐related infections by offering a combined active antimicrobial and immunotherapeutic strategy, thereby significantly reducing the recurrence rate following recovery from implant‐associated infections.
A Biomaterial‐Based Hedging Immune Strategy for Scarless Tendon Healing
Authors: Wang Zhen , Xiang Lei , Lin Feng , Tang Yunkai , Deng Lianfu , Cui Wenguo 
Published: March 2022

AbstractScarring rather than regeneration, is an inevitable outcome of unbalanced amplifications of inflammation‐destructive signals and atresia of the regenerative niche. However, identifying and effectively hedging against the risk of scarring and realizing the conversion of regenerative cues remain difficult. In this work, a hedging immune strategy based microfibrous membrane (Him‐MFM), by tethering distearoyl phosphoethanolamine layer‐supported copoly(lactic/glycolic acid) electrospun fibers with identified CD11b+/CD68+scarring subpopulation membranes in the immune landscape after tendon injury to counterweigh tissue damage, is reported. Him‐MFM, carrying relevant risk receptors is shown to shift high type I biased polarization, alleviate apoptosis and metabolic stress, and mitigate inflammatory tenocyte response. Remarkably, the hedging immune strategy reverses the damaged tendon sheath barrier to the innate IL‐33 secretory phenotype by 4.36 times and initiates the mucous‐IL‐33‐Th2 axis, directly supplying a transient but obligate regenerative niche for sheath stem cell proliferation. In murine flexor tendon injury, the wrapping of Him‐MFM alleviates pathological responses, protects tenocytes in situ, and restores hierarchically arranged collagen fibers covered with basement membrane, and is structurally and functionally comparable to mature tendons, demonstrating that the hedging immunity is a promising strategy to yield regenerative responses not scarring.
Polydopamine Decorated Microneedles with Fe‐MSC‐Derived Nanovesicles Encapsulation for Wound Healing
Authors: Ma Wenjuan , Zhang Xiaoxuan , Liu Yuxiao , Fan Lu , Gan Jingjing , Liu Weilin , Zhao Yuanjin , Sun Lingyun 
Published: March 2022

AbstractWound dressing with the capacities of antioxidation, antiinflammation, and efficient angiogenesis induction is expected for effectively promoting wound healing. Herein, a novel core‐shell hyaluronic acid (HA) microneedle (MN) patch with ferrum‐mesenchymal stem cell‐derived artificial nanovesicles (Fe‐MSC‐NVs) and polydopamine nanoparticles (PDA NPs) encapsulated in the needle tips is presented for wound healing. Fe‐MSC‐NVs containing multifunctional therapeutic cytokines are encapsulated in the inner HA core of the MN tips for accelerating angiogenesis. The PDA NPs are encapsulated in the outer methacrylated hyaluronic acid (HAMA) shell of the MN tips to overcome the adverse impacts from reactive oxygen species (ROS)‐derived oxidative stress. With the gradual degradation of HAMA patch tips in the skin, the PDA NPs are sustainably released at the lesion to suppress the ROS‐induced inflammation reaction, while the Fe‐MSC‐NVs significantly increase the migration, proliferation, and tube formation of human umbilical vein endothelial cells (HUVEC). More attractively, the combination of PDA NPs and Fe‐MSC‐NVs further promotes M2 macrophage polarization, thereby suppressing wound inflammation. Through in vivo experiment, the Fe‐MSC‐NVs/PDA MN patch shows an excellent effect for diabetic wound healing. These features of antioxidation, antiinflammation, and pro‐angiogenesis indicate the proposed composite core‐shell MN patch is valuable for clinical wound healing applications.
The stability of youth popular opinion leaders selected over time using social network analysis
Authors: Waterman Emily A. , Edwards Katie M. , Keyes Antoni B. , Zulfiqar Hafsa , Banyard Victoria L. , Valente Thomas W. 
Published: March 2022

AbstractCommunity interventions that use social network analysis to identify and involve influential individuals are promising for behavior change. However, youth friendships are often unstable. The current study examined the stability of the youth selected as influential in a friendship social network, that is, the degree to which youth selected at one time point were also selected at subsequent time points. Influential youth, also called popular opinion leaders (POLs), were selected to be part of a community‐wide sexual violence prevention initiative. POLs were selected based on high in‐degree (number of times an individual was nominated as a best friend by another student). We found that POLs were unstable: only 29.81%–41.01% of POLs were stable across time. The percentage of POLs who were stable decreased across time. No factors (social identities, behavioral, attitudinal) consistently predicted POL stability. Although these findings are in need of replications, social network interventions for youth may need to repeatedly select new POLs to account for instability.
Identical EP300 variant leading to Rubinstein–Taybi syndrome with different clinical and immunologic phenotype
Authors: Saettini Francesco , Fazio Grazia , Bonati Maria Teresa , Moratto Daniele , Massa Valentina , Di Fede Elisabetta , Castiglioni Silvia , Marchetti Daniela , Chiarini Marco , Sottini Alessandra , Iascone Maria , Cazzaniga Giovanni , Imberti Luisa , Biondi Andrea , Gervasini Cristina , Badolato Raffaele 
Published: March 2022

AbstractThe Rubinstein–Taybi syndrome (RSTS) is a rare developmental disorder characterized by craniofacial dysmorphisms, broad thumbs and toes, intellectual disability, growth deficiency, and recurrent infections. Mutations in the cyclic adenosine monophosphate response element‐binding protein (CREB)‐binding protein (CREBBP) or in the E1A‐associated protein p300 (EP300) genes have been demonstrated in 55% (RSTS1) and up to 8% of the patients (RSTS2), respectively. Dysfunction of immune response has been reported in a subgroup of individuals with RSTS. Here we characterize two patients carrying the same EP300 variant and distinctive RSTS features (including congenital heart abnormalities, short stature, feeding problems, and gastroesophageal reflux). Whole exome sequencing did not support a dual molecular diagnosis hypothesis. Nonetheless, patients showed distinct clinical manifestations and immunological features. The most severe phenotype was associated with reduced T‐cell production and diversity. This latter feature was confirmed in a control group of four RSTS patients.
Circularly Polarized White Organic Light‐Emitting Diodes Based on Spiro‐Type Thermally Activated Delayed Fluorescence Materials
Authors: Zhang Yi‐Pin , Mao Meng‐Xi , Song Shi‐Quan , Wang Yi , Zheng You‐Xuan , Zuo Jing‐Lin , Pan Yi 
Published: March 2022

AbstractIn this study, we report the first circularly polarized white organic light‐emitting diodes (CP‐WOLEDs) based on all thermally activated delayed fluorescence (TADF) materials. Two pairs of spiro‐type TADF enantiomers, (R/S)‐SPOCN (5,5′‐((2,2′,3,3′‐tetrahydro‐1,1′‐spirobi[indene]‐7,7′‐diyl)bis(oxy))bis(4‐(10H‐phenoxazin‐10‐yl)phthalonitrile)) and (R/S)‐OSFSO (2′‐(trifluoromethyl)‐spiro[quinolino[3,2,1‐kl]phenoxazine‐9,9′‐thioxanthene]‐10′,10′‐dioxide), serve as emitters with complementary emission. The CP‐OLEDs exhibit warm white emission with a CIE coordinate of (0.35, 0.46). Besides, decent device performances are observed with an external quantum efficiency of up to 21.6 % at maximum and 11.8 % at 1000 cd m−2. Obvious circularly polarized electroluminescence signals are detected with a dissymmetry factor |gEL| of around 3.0×10−3. This is the first report of CP‐WOLEDs that can harvest both singlet and triplet excitons, which provides a feasible strategy for the development of CP‐WOLEDs with remarkable device performances.
Enantiomeric Water‐Soluble Octopus[3]arenes for Highly Enantioselective Recognition of Chiral Ammonium Salts in Water
Authors: Han Xiao‐Ni , Li. Peng‐Fei , Han Ying , Chen Chuan‐Feng 
Published: March 2022

AbstractStudy of enantioselective recognition in water by synthetic chiral macrocyclic receptors is undoubtedly of theoretical and practical significance, but it is a big challenge to achieve the enantioselective recognition with both high enantioselectivity and high affinity in water probably due to the deficiency of such water‐soluble macrocyclic receptors with stable chiral cavities. Herein, we report a new class of chiral macrocyclic arenes named octopus[3]arenes. The enantiomeric macrocycles are composed of three homochiral ethenoanthracene subunits, and they can be synthesized by two pathways and then easily converted into water‐soluble octopus[3]arenes P‐1 and M‐1. Notably, P‐1 and M‐1 with the rigid hexagonal structures and stable chiral hydrophobic cavities exhibit highly enantioselective recognition towards three pairs of chiral ammonium salts in water with the association constant up to 106 M−1 and the S/R selectivity up to 12.89.
Sizing carbon fiber by in situ polymerization of maleic acid and glycerol for reinforcing polyamide 66
Authors: Yao Lili , Lu Yonggen , Zhang Chenyang , Yang Shulei , Yang Changling 
Published: March 2022

AbstractThe interfacial adhesion character highly depends on interactions of the interfacial molecules. Carbon fiber (CF) is sized by in situ polymerization of maleic acid and glycerol to form a hyperbranched polyester (HBPE) layer on the surface in an environmental and efficient technique. Vast hydroxyl groups are introduced to the CF surface with the presence of HBPE. Moreover, the HBPE sizing structure on 6 A/m2 moderately oxidized CF surface presents higher branch extent and more terminal hydroxyl groups than that on unoxidized or over‐oxidized CF surface. The interlaminar shear strength of polyamide 66 composite reinforced with HBPE sized oxidized‐CF under 6 A/m2 current density is optimal and remarkably enhanced to 77.9 MPa by 46% compared to that with the unsized one. The significant interfacial property improvement is attributed to the abundant terminal hydroxyl groups on HBPE sizing structure, which enhances the interaction at the interface of CF/sizing and sizing/matrix by constructing many covalent and hydrogen bonds.
Unusual SMG suspects recruit degradation enzymes in nonsense‐mediated mRNA decay
Authors: Gilbert Agathe , Saveanu Cosmin 
Published: March 2022

AbstractDegradation of eukaryotic RNAs that contain premature termination codons (PTC) during nonsense‐mediated mRNA decay (NMD) is initiated by RNA decapping or endonucleolytic cleavage driven by conserved factors. Models for NMD mechanisms, including recognition of PTCs or the timing and role of protein phosphorylation for RNA degradation are challenged by new results. For example, the depletion of the SMG5/7 heterodimer, thought to activate RNA degradation by decapping, leads to a phenotype showing a defect of endonucleolytic activity of NMD complexes. This phenotype is not correlated to a decreased binding of the endonuclease SMG6 with the core NMD factor UPF1, suggesting that it is the result of an imbalance between active (e.g., in polysomes) and inactive (e.g., in RNA‐protein condensates) states of NMD complexes. Such imbalance between multiple complexes is not restricted to NMD and should be taken into account when establishing causal links between gene function perturbation and observed phenotypes.
Coronavirus disease 2019 (COVID‐19) as a potential trigger for benign recurrent intrahepatic cholestasis
Authors: Çalhan Turan , Yivli Elif 
Published: March 2022

AbstractBenign recurrent intrahepatic cholestasis (BRIC) is a rare disease characterized by recurrent severe itching and jaundice. Coronavirus disease 2019 (COVID‐19) is a multisystemic acute viral disease and the liver is frequently affected. Here, we wanted to present a BRIC case triggered by COVID‐19 infection, discussing it together with current information.
Acid Phosphatase Immobilization and Production Study by Trichoderma spp. in Soybean Molasses
Authors: Alves Lima Frederico , Rola Juliana Cristina , Gomes de Freitas Matheus Marçal , de Almeida Afonso Jussara Maria Martins , de Resende Miriam Maria 
Published: March 2022

AbstractAcid phosphatase production was carried out by Trichoderma spp. using treated soybean molasses at different concentrations. Results showed higher acid phosphatase activity when the soybean molasses concentration increased for the microorganisms Trichoderma harzianum and Trichoderma reesei. The acid phosphatase activity performance of Trichoderma asperellum was also evaluated. In general, the polyethyleneimine glyoxyl agarose support showed much better performance and final recovery of activity than DEAE Toyopearl 650s.