bluuebunny/crossref_metadata_2025_split huggingface dataset:
lncRNA NEAT1-let 7b-P21 axis mediates the proliferation of neural stem cells cultured in vitro promoted by radial extracorporeal shock wave
Authors: Han Kun , Kang Nan , Yu Xiaotong , Lu Jie , Ma Yuewen 
Published: January 2022

Abstract
In previous studies, we found radial extracorporeal shock wave (rESW), can promote the proliferation of neural stem cells（NSCs）. Emerging evidence suggests that lncRNA NEAT1 can regulate NSCs proliferation. Whether lncRNA NEAT1 plays a role in the proliferation of NSC induced by shock waves is unclear. Cell Counting Kit-8（CCK 8） method was used to detect the proliferation of NSCs, and the relative protein and mRNA expression of related genes of Nestin, Cyclin D1 and P21 were detected by Western Blot and Quantitative real-time PCR（RT-qPCR）respectively. Immunofluorescence staining was used to observe the changes in the number of BrdU/nestin positive cells. Overexpression of NEAT1 and let 7b in cells were used to explore whether rESW can rescue the decreased number of NSCs.We found that the optimal dose of R15 transmitter promoting NSCs proliferation is 1.5 bar, 500 pulse, 2 Hz. 1.2-1.5bar showed a dose-dependent effect on the proliferation of NSCs, but it was negatively correlated with the proliferation effect of NSC when it was more than 1.5bar. We revealed that let 7b-P21 axis was involved in regulating the inhibition of NSC proliferation which was activated by NEAT1 in NSCs. In addition, we demonstrated that rESW treatment resulted in the decrease of NEAT1 expression, which was accompanied by the improved biological function including proliferation.Our results confirm that low-intensity rESW（1.5bar，500pulse，2Hz） can promote the proliferation of NSCs through NEAT1-let 7b-P21 axis.
Role of Magnetite, Rutile and H2S in C-O-H Fluids’ Speciation: Implications For Localized Reduction Zones in the Shallow Upper Mantle
Authors: Choudhary Shubham , Das Souvik , Sen Koushik , Kumar Santosh 
Published: January 2022

Abstract
Carbon (C) and sulfur (S) bearing volatiles in the mantle are important as they control processes like metasomatism and melting that ultimately influence Earth’s geochemical cycle. Here we report rare shallow mantle fluid inclusions in a pyroxenite, associated with the Kerguelen Plume, from Northeast India. Optical microscopy and Raman spectroscopy reveal primary inclusions of hydrocarbon (CH) fluid and calcite in diopsidic clinopyroxene (Cpx) of this pyroxenite. Another Cpx from the same rock shows a rare occurrence of pseudo-secondary polyphase fluid inclusions of hydrogen sulfide (H2S), carbon monoxide (CO), rutile and calcite. We infer that metasomatic interaction between silicates and C-O-H volatiles generated these fluids, which are trapped as inclusions. We further suggest two factors controlling carbon speciation in these fluids 1) exsolution of magnetite and diffusion of Ti from the Cpx to form rutile and 2) oxidation buffering due to presence of H2S that prevented the formation of CH and formed CO instead. Our study explains the existence of significant reduction zones at localized scale in the oxidized shallow upper mantle.
Analysis of CircRNAs and CircRNA-Associated Competing Endogenous RNA Networks in β-Thalassemia
Authors: Yang Fang , Li Shuquan , Ruan Heyun , Hou Wei , Qiu Yuling , Deng Lingjie , Su Sha , Chen Ping , Pang Lihong , Lai Ketong 
Published: January 2022

Abstract
The involvement of circRNAs in β-thalassemia and their actions on fetal hemoglobin (HbF) is unclear. Here, the circRNAs in β-thalassemia carriers with high HbF levels were comprehensively analyzed in comparison with healthy individuals. Differential expression of 2183 circRNAs was observed and their correlations with hematological parameters were investigated. Down-regulated has-circRNA-100466 had a strong negative correlation with HbF and HbA2. Bioinformatics was employed to construct a has-circRNA-100466‑associated competing endogenous RNA (ceRNA) network with the determination of hub genes and associated miRNAs. In combination with previous reports, the has-circRNA-100466▁miR-19b-3p▁SOX6 pathway was identified. The ceRNA network was verified by qRT-PCR on β-thalassemia samples and RNA immunoprecipitation of K562 cell lysates. Has-circRNA-100466, miR-19b-3p, and SOX6 were present together in anti-argonaute 2 immunoprecipitates, indicating involvement with HbF induction. Furthermore, spearman correlation coefficients revealed their significant correlations with HbF. In conclusion, a novel has-circRNA-100466▁miR-19b-3p▁SOX6 pathway was identified, providing insight into HbF induction and suggesting targets β-thalassemia treatment.
Glucose-6-Phosphate Dehydrogenase is a Prognostic Biomarker and Correlated with Immune Infiltrates in Hepatocellular Carcinoma
Authors: Bu Yang , Liu Kejun , Niu Yiming , Hao Ji , Cui Lei , Li Cunquan , Chen Bendong 
Published: January 2022

Abstract
Background: Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in the metabolic and immunological aspects of tumors. In hepatocellular carcinoma (HCC), the alteration of tumor microenvironment influences recurrence and metastasis. We extracted G6PD-related data from public databases of HCC tissues and used a bioinformatics approach to explore the correlation between G6PD expression and clinicopathological features and prognosis of immune cell infiltration in HCC.Methods: We extract G6PD expression information from TCGA and GEO databases in liver cancer tissues and normal tissues, validated by immunohistochemistry, and the correlation between G6PD expression and clinical features is analyzed, and the clinical significance of G6PD in liver cancer is assessed by Kaplan-Meier, Cox regression and prognostic line graph models. Functional enrichment analysis is performed by protein-protein interaction (PPI) network, GO/KEGG, GSEA and G6PD-associated differentially expressed genes (DEGs). TIMER and ssGSEA packages are used to assess the correlation between expression and the level of immune cell infiltration.Results: Our results show that G6PD expression is significantly upregulated in hepatocellular carcinoma tissues (P < 0.001). G6PD expression is associated with histological grade, pathological stage, T-stage, vascular infiltration and AFP level (P < 0.05); HCC patients in the low G6PD expression group had longer overall survival and better prognosis compared with the high G6PD expression group (P < 0.05). The level of G6PD expression also affects the levels of macrophages, unactivated dendritic cells, B cells, and follicular helper T cells in the tumor microenvironment.Conclusion: High expression of G6PD is a potential biomarker for poor prognosis of hepatocellular carcinoma, and G6PD may be a target for immunotherapy of HCC.
TNFRSF9 Suppressed the Progression of Breast Cancer via the p38MAPK/PAX6 Signaling Pathway
Authors: Zhu Xun , Liu Xiaorong , Zhou Yehui , Qin Chenglin 
Published: January 2022

Abstract
Background Worldwide, Breast cancer is the most common cancer in females. Endocrine therapy can effectively treat 85% of breast cancer patients, but 15% of patients could only be treated with chemotherapy and surgery, and the prognosis is much worse. Immunotherapy is the novel treatment for breast cancer that PD-1 and CTLA-4 antibodies have shown evidence of immune modulation in breast cancer drug trials. Methods and Result In this study, we report that TNFRSF9 regulates the cell proliferation, invasion, and apoptosis of breast cancer cells through regulating the phosphorylation of p38, thus further regulate the expression of PAX6. In both breast cancer tissues and cell lines, the levels of TNFRSF9 are significantly decreased, and breast cancer cell development will be promoted with knockdown of TNFRSF9. Moreover, we identify that downregulation of TNFRSF9 can upregulate the phosphorylated p38 (p-p38) and PAX6. We further elucidate that p-p38 is essential for PAX6 expression that p38 phosphorylation inhibitor can reverse the upregulation of PAX6 and suppress cell proliferation, invasion, and promote apoptosis in breast cancer cells. Conclusions In summary, this study proposed a novel TNFRSF9/p38/PAX6 axis that contributes to tumor suppression, which suggests a potential immunotherapy target for breast cancer.
New fossil assemblages from the Early Ordovician Fezouata Biota
Authors: Saleh Farid , Vaucher Romain , VIDAL Muriel , HARIRI Khadija EL , Laibl Lukáš , Daley Allison , Gutiérrez-Marco Juan Carlos , Candela Yves , Harper David , Ortega-Hernández Javier , Ma Xiaoya , Rida Ariba , Vizcaïno Daniel , Lefebvre Bertrand 
Published: January 2022

Abstract
The Fezouata Biota (Morocco) is an exceptionally well-preserved fossil community of Early Ordovician age and although its oldest units are comparable with Burgess Shale-type localities of the Cambrian Explosion, little attention has been paid to the younger units despite potential to reveal the conditions of the Ordovician Radiation. Herein, we describe a new middle to upper Floian Fezouata locality (Taichoute) encompassing an assemblage dominated by large bivalved euarthropods and giant filter-feeding radiodonts, which were transported and preserved in concretions associated with density-flow deposits. Taichoute captures the closing of the taphonomic window that characterizes exceptional fossil preservation during the Cambrian Explosion (i.e., carbonaceous compressions) as well as the faunal transition to assemblages dominated by typical Palaeozoic taxa.
Efficacy and Safety of the Combination of Nano-Liposomal Irinotecan and 5-Fluorouracil/L-Leucovorin in Unresectable Advanced Pancreatic Cancer: A Real-World Study
Authors: Yasuoka Hidetoshi , Naganuma Atsushi , Kurihara Eishin , Kobatake Tsutomu , Ijima Masashi , Tamura Yuki , Suzuki Yuhei , Hoshino Takashi , Hosaka Hisashi , Hatanaka Takeshi , Yoshida Sachiko , Aihara Ryusuke , Hosouchi Yasuo , Ishii Norihiro , Araki Kenichiro , Shirabe Ken , Kakizaki Satoru 
Published: January 2022

Abstract
Aim: This retrospective study investigated the efficacy and safety of nano-liposomal irinotecan (nal-IRI) plus 5-fluorouracil/l-leucovorin (5-FU/l-LV) treatment in the second-line or later setting for advanced pancreatic cancer under real-world conditions.Methods: Between June 2020 and September 2021, a total of 44 patients with unresectable advanced pancreatic cancer treated with nal-IRI + 5-FU/l-LV in our affiliated hospitals were included. The prognosis, predictive factors (including systemic inflammation-based prognostic indicators), and adverse events were investigated.Results: The median age was 68 (interquartile range [IQR] 62-73) years old, and 22 patients (50.0%) were male. Concerning tumor factors, 9 patients (20.5%) had local advanced disease, and 35 patients (79.5%) had metastases. Twenty-five of the 44 patients were receiving second-line treatment, and 19 were receiving third-line or later treatment. The median overall survival (OS) and progression free survival (PFS) were 9.0 (range, 0.7-15.4) months and 4.4 (range, 0.6-15.4) months, respectively. The overall response rate (ORR) was 5.3%. The disease control rate (DCR) was 44.7%. Patients with a neutrophil-to-lymphocyte ratio (NLR) of >2.7 had a significant risk of a poor OS (HR=0.275, P=0.017). Adverse events were manageable, although gastrointestinal symptoms and neutropenia were observed. The most common grade ≥3 adverse event was neutropenia, which was reported in 20% of patients.Conclusions: Nal-IRI + 5-FU/l-LV therapy was considered to be a useful regimen as second-line or later treatment for unresectable advanced pancreatic cancer, even in clinical practice.
Design, Synthesis of Amide Derivatives of Scutellarin And Their Anti-Leukemia And Neuroprotective Activities
Authors: Han Tong , Jiang Chunyu , Wei Xing , Sheng Meilin , Xie Qin , Zhang Jiqiang , Zhang Yongyi , Jin Chenghao 
Published: January 2022

Abstract
A unique series of amide-scutellarin derivatives were designed and synthesized in order to develop the function of scutellarin further. The antiproliferative activity of all target compounds against two human leukaemia cell lines were evaluated. Among them, compounds 6g and 7c displayed the most antitumor activities against HL-60 and THP-1. Moreover, all compounds were also assayed for their neuroprotective activity against hydrogen peroxide (H2O2)-induced PC-12 cell injury, and the majority of the compounds had moderate to good neuroprotective properties. These findings confirmed that these target compounds could be used as anti-leukaemia and neuroprotective candicates in the future.
Energy-aware and Carbon-efficient VM Placement Optimization in Cloud Datacenters Using Evolutionary Computing Methods
Authors: Abbasi-khazaei Tahereh , Rezvani Mohammad Hossein 
Published: January 2022

Abstract
One of the most important concerns of cloud service providers is balancing renewable and fossil energy consumption. On the other hand, the policy of organizations and governments is to reduce energy consumption and greenhouse gas emissions in cloud data centers. Recently, a lot of research has been conducted to optimize the Virtual Machine (VM) placement on physical machines to minimize energy consumption. Many previous studies have not considered the deadline and scheduling of IoT tasks. Therefore, the previous modelings are mainly not well-suited to the IoT environments where requests are time-constraint. Unfortunately, both the sub-problems of energy consumption minimization and scheduling fall into the category of NP-hard issues. In this study, we propose a multi-objective VM placement to joint minimizing energy costs and scheduling. After presenting a modified memetic algorithm, we compare its performance with baseline methods as well as state-of-the-art ones. The simulation results on the CloudSim platform show that the proposed method can reduce energy costs, carbon footprints, SLA violations, and the total response time of IoT requests.
Effects of Transcranial Magneto-acoustic Electrical Stimulation on Discharge propagation in feed-forward neural network
Authors: Wu Jiankang , Zhang Shuai , Xu Jiayue , Dang Junwu , Zhao Qingyang , Zhao Mingkang , Xu Guizhi , Zhang Xueying , Wang Hongbin , Du Wenjing 
Published: January 2022

Abstract
The mammalian brain has an extremely complex, diversified and highly modular structure, and information dissemination in the modular brain network affects various brain diseases. Although a variety of neuromodulation techniques have been used to study the discharge characteristics of neural networks, the effects of transcranial magneto-acoustic electrical stimulation(TMAES) have rarely been mentioned. Based on the excitatory and inhibitory Izhikevich neuron model, we constructs a feed-forward neural network connected by electrical synapses and chemical synapses, and analyzes the firing frequency of the neural network under TMAES and magnetic stimulation and the differences in each layer types of firing patterns of neurons. The results showed that the discharge patterns of neurons in each layer were different, the discharge frequency of inhibitory neurons was higher than that of excited neurons, and the stimulation signal could be transmitted to the whole network layer.The maximum discharge frequency of neural network connected by electrical coupling can reach 0.94kHz, and the discharge frequency of neural network connected by chemical coupling is less than 0.5 kHz.With the increase of coupling degree, the discharge frequency of neurons in each network layer under TMAES is much greater than that under magnetic stimulation.When the induced current is less than 26.5μA/cm 2 , magnetic stimulation can promote the inhibitory neurons, and TMAES has a variety of regulatory effects on the inhibitory neurons in the neural network. The results show that TMAES has better regulation effect than magnetic stimulation, and the regulation effect is affected by neural network structure and stimulation parameters.
A Retrospective Analysis of Serous Effusions Based on the Newly Proposed International System for Reporting Serous Fluid Cytopathology: A Report of 3633 Cases in an Oncological Center
Authors: Zhu Yan-li , Ren Wen-hao , Wang Qian , Jin Hai-zhu , Guo Yi-yi , Lin Dong-mei 
Published: January 2022

Abstract
Background: The International System for Reporting Serous Fluid Cytopathology (TIS) was recently proposed. We retrospectively applied TIS recommendations for reporting the cytological diagnosis of serous effusions and reported our experience.Methods: All the serous effusions from January 2018 to September 2021 were retrieved from the database. Recategorization was performed using the TIS classification, the risk of malignancy (ROM) was calculated for each TIS category, and the performance evaluation was carried out among different samples (pleural, peritoneal and pericardial effusions) and preparation methods (conventional smears, liquid-based preparations and cell-blocks). Results: A total of 3633 cases were studied, 17 (0.5%) were diagnosed as ND, 1100 (30.3%) as NFM, 101 (2.8%) as AUS, 677 (18.6%) as SFM, and 1738 (47.8%) as MAL. The ROM for the categories were found to be 38.5%, 28.6%, 52.1%, 99.4% and 100%, respectively. The ROM for SFM was significantly higher than that for AUS (P < 0.001), while the difference between the ROMs for the IVa and IVb was insignificant. The sensitivity, negative predictive value(NPV) and diagnostic accuracy of liquid-based preparations were all superior to conventional smears and cell-blocks in detecting abnormalities. Using three preparation methods simultaneously had the highest sensitivity, NPV and diagnostic accuracy.Conclusion: Serous effusion cytology has a high specificity and positive predictive value(PPV), and the TIS is a user-friendly reporting system. Liquid-based preparations could improve the sensitivity of diagnosis, and it is best to use three different preparation methods simultaneously for serous effusion cytologic examination.
Detection of Suspended SARS-CoV-2 in Indoor Air Using an Electrostatic Sampler
Authors: Fukuda Kyohei , Yoshida Mie , Noto Kensuke , Kitabayashi Kouichi , Katsushima Shinjirou , Sonehara Hiroki , Mizuno Kazue , Mizuno Akira 
Published: January 2022

Abstract
A prototype virus sampler using electrostatic precipitation has been developed to investigate aerosol infection by SARS-CoV-2. The sampler consists of a discharge electrode placed inside a vial, and a thin layer of viral lysis buffer at the bottom, working as a collection electrode. The sampler was operated with the sampling air flow rate of 40 L/min. Collection efficiency of the sampler is about 80% for 25nm to 5.0µm diameter particles. We sampled the air of a food court of a commercial facility, a connecting corridor of a clouded train station, and two office rooms (A and B) in September 2021, just after the 5th peak of COVID-19 in Japan. The analysis using a RT-qPCR detected the virus RNA in the air of the office A, B and the food court. Estimated concentration of the virus in the air determined by calibration curve was 2.0 x 102, 7.8 x 102, and 0.6 - 2.4 x 102 copies/m3, in the office A, B, and the food court, respectively. These results indicate that the sampler using electrostatic precipitation can detect SARS-CoV-2 in indoor air. It could be developed as a risk assessment method for aerosol infection.
Economics of wheat as influenced by natural farming, organic farming and recommended package of practices
Authors: Behera Alok , P Chandrashekara C 
Published: January 2022

Abstract
A field experiment was laid out on split-plot design with two uneven controls viz. four foliar concentrations of cow urine and jeevamrutha under natural farming which were compared with Organic farming (OF) and Recommended package of practices (RPP) given by UAS, Dharwad. The treatments were replicated thrice. The study revealed that RPP recorded significantly higher grain yield, straw yield, gross return, net return and B:C ratio than OF and natural farming practices. The grain yield reduction in the best treatment (cow urine @ 50 % + jeevamrutha @ 100 %) was 16 % lesser than RPP and 2 % higher than OF. However, the cost of cultivation in natural farming with cow urine @ 50 % + jeevamrutha @ 100 % was lesser to the extent of 6.91 and 30.42 % than RPP and OF. Our study indicates that the irrigated wheat can be grown under natural farming conditions profitably.
Does a One Health Approach to Human African Trypanosomiasis Control Hasten Elimination? A Stochastic Compartmental Modeling Approach
Authors: Meisner Julianne , Kato Agapitus , Lemerani Marshall , Miaka Erick Mwamba , Taban Acaga Ismail , Wakefield Jonathan , Rowhani-Rahbar Ali , Pigott David , Mayer Jonathan , Lorton Christopher , Rabinowitz Peter 
Published: January 2022

Abstract
Background: In response to large strides in the control of human African trypanosomiasis (HAT), in the early 2000s the WHO set targets for elimination of both the gambiense (gHAT) and rhodesiense (rHAT) forms as a public health (EPHP) problem by 2020, and elimination of gHAT transmisson (EOT) by 2030. While global EPHP targets have been met, and EOT appears within reach, there is ample evidence that current control strategies will not achieve gHAT EOT in the presence of animal reservoirs, the role of which is currently uncertain. Furthermore, rHAT is not targeted for EOT due to the known importance of animal reservoirs for this form. Methods: To evaluate the utility of a One Health approach to gHAT and rHAT EOT, we built and parameterized a compartmental stochastic model, using the Institute for Disease Modeling's Compartmental Modeling Software, to six HAT epidemics: the national rHAT epidemics in Uganda and Malawi, the national gHAT epidemics in Uganda and South Sudan, and two separate gHAT epidemics in Democratic Republic of Congo distinguished by dominant vector species. In rHAT foci the reservoir animal sub-model was stratified on four species groups, while in gHAT foci domestic swine were assumed to be the only competent reservoir. The modeled time horizon was 2005-2045, with calibration performed using HAT surveillance data from 2000-2004 and Optuna. Interventions included insecticide and trypanocide treatment of domestic animal reservoirs at varying coverage levels. Results: Validation against HAT surveillance data indicates favorable performance overall, with the possible exception of DRC. EOT was not observed in any modeled scenarios for rHAT, however insecticide treatment consistently performed better than trypanocide treatment in terms of rHAT control. EOT was not observed for gHAT at 0% coverage of domestic reservoirs with trypanocides or insecticides, but was observed by 2030 in all test scenarios; again, insecticides demonstrated superior performance to trypanocides. Conclusions: EOT cannot be achieved for rHAT without control of wildlife reservoirs, however insecticide treatment of domestic animals holds promise for improved control. In the presence of domestic animal reservoirs, gHAT EOT will not be achieved under current control strategies.