bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of 3‐month follow‐up of patients with postacute COVID‐19 syndrome
Authors: Kerget Buğra , Çelik Eda , Kerget Ferhan , Aksakal Alperen , Uçar Elif Yılmazel , Araz Ömer , Akgün Metin 
Published: January 2022

AbstractIn addition to the highly variable clinical presentation of acute COVID‐19 infection, it can also cause various postacute signs and symptoms. This study aimed to evaluate patients with postacute COVID‐19 over 12 weeks of follow‐up. The study included 151 patients who were diagnosed with COVID‐19 by real‐time polymerase chain reaction of a nasopharyngeal swab 1 month earlier, had radiologic findings consistent with COVID‐19 pneumonia, and presented to the post‐COVID‐19 outpatient clinic between May and August 2021. The patients were divided into three groups based on COVID‐19 severity: nonsevere pneumonia (Group 1), severe pneumonia (Group 2), and severe pneumonia requiring intensive care (Group 3). Evaluation of laboratory parameters at 4 and 12 weeks showed that Group 3 had a higher lactose dehydrogenase (LDH) level and a lower mean platelet volume than the other groups at both time points (p = 0.001 for all). Group 3 also had lower percent predicted forced vital capacity (FVC%), percent predicted forced expiration volume in 1 s (FEV1%), and percent predicted diffusion capacity of the lungs for carbon monoxide divided by alveolar volume (DLCO/VA%) compared to Groups 1 and 2 at Week 4 (p = 0.001, 0.004, 0.001, respectively) and compared to Group 1 at 12 weeks (p = 0.002, 0.03, 0.001, respectively). Patients with persistent dyspnea at 12 weeks had significantly lower FEV1%, FVC%, DLCO/VA%, and saturation levels in room air and significantly higher LDH, pro‐BNP, D‐dimer, and heart rate compared to those without dyspnea (p = 0.001 for all). Although the lungs are most commonly affected after COVID‐19 infection, vascular and endothelial damage also causes multisystem involvement. Our study indicates that laboratory values, radiological signs, and pulmonary functional capacity improved in most patients after 12 weeks of follow‐up.
Associations of human papillomavirus (HPV) genotypes and related risk factors in a cohort of women living with HIV in a Brazilian countryside city
Authors: Kury Charbell M. H. , Guimaraes Gabriela R. , Leandro Cinthia G. , Fernandes Lorena A. , Silva Paulo Ricardo T. , M. B. Petraglia Tania Cristina , Cruz Oswaldo G. , da Costa Carneiro Rodrigo , Cavalcanti Silvia M. B. 
Published: January 2022

AbstractThe human papillomavirus (HPV) is associated with cervical abnormalities. People living with HIV are more susceptible to HPV. Campos dos Goytacazes implemented the quadrivalent HPV vaccine (4vHPV) for women living with HIV (WLWH) in 2011, 4 years before the Brazilian public vaccination program. We aimed to characterize the genomic diversity and predictors of HPV infection in WLWH through a prospective cohort study. After the consent form was received, a questionnaire was applied and an endocervical sample was collected. For genotyping, a microarray HPV technique was performed. Two intervention moments were performed: T1, the initial moment, with collection and vaccination; T2 moment, 2 years after T1. Univariate and multivariate analyses were performed. The T1 moment cohort was formed by 146 women,107 belonging to Group 1(HPV‐negative) and 39 to Group 2 (HPV‐positive). The variables age, marital status, number of children, number of sexual partners, and CD4 count were protective against HPV. The variables number of sexual partners, marital status, and the number of children lost significance in multivariate analysis. Concerning T2 moment, 42 patients were followed with three positive cases. The use of 4vHPV is beneficial for this population and should also be recommended at an age from 26 to 45 years inside the public vaccination program.
Generation, characterization, and protective ability of mouse monoclonal antibodies against the HA of A (H1N1) influenza virus
Authors: Wang Liyan , Yang Fan , Xiao Yixin , Chen Bin , Liu Fumin , Cheng Linfang , Yao Hangping , Wu Nanping , Wu Haibo 
Published: January 2022

AbstractInfluenza virus infections pose a continuous threat to human health. Although vaccines function as a preventive and protective tool, they may not be effective due to antigen drift or an inaccurate prediction of epidemic strains. Monoclonal antibodies (mAbs) have attracted wide attention as a promising therapeutic method for influenza virus infections. In this study, three hemagglutinin (HA)‐specific mAbs, named 2A1, 2H4, and 2G2, respectively, were derived from mice immunized with the HA protein from A/Michigan/45/2015(H1N1). The isolated mAbs all displayed hemagglutination inhibition activity and the 2G2 mAb exhibited the strongest neutralization effect. Two amino acid mutations (A198E and G173E), recognized in the process of selection of mAb‐resistant mutants, were located in antigenic site Sb and Ca1, respectively. In prophylactic experiments, all three mAbs could achieve 100% protection in mice infected with a lethal dose of A/Michigan/45/2015(H1N1). A dose of 1 mg/kg for 2H4 and 2G2 was sufficient to achieve a full protective effect. Therapeutic experiments showed that all three mAbs could protect mice from death if they received the mAb administration at 6 h postinfection, and 2G2 was still protective after 24 h. Our findings indicate that these three mAbs may have potential prevention and treatment value in an H1N1 epidemic, as well as in the study of antigen epitope recognition.
Married women's career orientation and life satisfaction: Role of self‐efficacy
Authors: Yang Xinfeng , Choi Byoung Kwon 
Published: January 2022

AbstractThis study conducts an in‐depth exploration of the ways career orientation is related to life satisfaction by considering job satisfaction as a mediator and self‐efficacy as a moderator. The study sample comprised 204 married women employees recruited in China through a self‐reported survey. We found that married women employees’ career orientation was positively related to life satisfaction through job satisfaction, and such an indirect influence was significant when they possessed high levels of self‐efficacy. The current research contributes to the collective understanding regarding how and when married women employees’ career orientation results in life satisfaction.
In vitro and in vivo effects of hyperglycemia and diabetes mellitus on nucleus pulposus cell senescence
Authors: Jiang Zengxin , Jiang Chang , Jin Lixia , Chen Zixian , Feng Zhenzhou , Jiang Xiaoxing , Cao Yuanwu 
Published: January 2022

AbstractDiabetes mellitus contributes to intervertebral disc degeneration. Nucleus pulposus cell senescence plays an important role in intervertebral disc degeneration. However, the effects of hyperglycemia on human nucleus pulposus cells and the underlying process remains poorly understood. In the current study, we evaluated the effects of high glucose levels on human nucleus pulposus cell senescence in vitro and the effects of hyperglycemia on rat nucleus pulposus aging in vivo. Human nucleus pulposus cells were cultured in high‐glucose medium (200 mM glucose) for 48 h. Senescence‐associated β‐galactosidase staining, western blot analysis, and enzyme‐linked immunosorbent assays were performed to evaluate human nucleus pulposus cell senescence. Flow cytometry and enzyme‐linked immunosorbent assays were used to evaluate reactive oxygen species and advanced glycation end‐product levels. Transcriptome sequencing followed by bioinformatics analysis was used to understand the abnormal biological processes of nucleus pulposus cells cultured in high‐glucose medium. Diabetes mellitus rat models were established and histopathological and immunohistochemical analysis was conducted to examine nucleus pulposus tissue senescence in vivo. Exposure to a high glucose concentration promoted human nucleus pulposus cell senescence and increased the senescence‐related secretion phenotype in human nucleus pulposus cells in vitro and in rat nucleus pulposus tissue in vivo. Bioinformatics analysis showed that hub genes were involved in nucleus pulposus cell cycle activities and cell senescence. The results suggest that appropriate blood glucose control may be key to preventing intervertebral disc degeneration in diabetic patients.
Knee symptoms do not affect walking biomechanics among women 6 months after anterior cruciate ligament reconstruction
Authors: Collins Katherine , Fajardo Ryan , Harkey Matthew , Knake Jeffrey , Lisee Caroline , Wilcox Luke , Tasco Jamie , Kuenze Christopher 
Published: January 2022

AbstractWomen with anterior cruciate ligament reconstruction report worse pain and knee‐related symptoms, and also exhibit biomechanical changes that may be related to knee osteoarthritis (OA) development. This is particularly concerning as symptom state has been previously associated with knee OA development. The purpose of this study was to compare lower extremity walking biomechanics between women (age: 21.40 ± 8.54 years) experiencing clinically significant knee‐related symptoms and women with acceptable symptoms 6 months following surgery. Twenty‐eight women with history of primary, unilateral anterior cruciate ligament reconstruction who completed a lower extremity walking biomechanics assessment 6 months following surgery were included in this analysis. Women were dichotomized as experiencing acceptable or clinically significant knee symptoms according to Knee injury and OA Outcomes Score cut‐offs described by Englund et al. Walking biomechanics were compared between women with clinically significant and acceptable symptoms using one‐way analysis of covariances for involved limb biomechanics. Biomechanical variables of interest were: peak vertical ground reaction forces (vGRFs), vGRF loading rates, knee flexion angles, knee extension moments, knee adduction angles, and knee adduction moments, and gait speed. Nearly 60% of women reported clinically significant knee symptoms 6 months postoperative. There were no statistically significant differences between symptom groups for walking biomechanics and gait speed outcomes. These findings suggest patient reported knee symptoms may not be a primary influence on walking biomechanics 6 months following anterior cruciate ligament reconstruction. Though, longitudinal assessment of changes in symptom state and walking biomechanics may be warranted as poorer walking biomechanics and symptoms are indicators of knee OA.
Intraoperative angiography imaging correlates with wound complications following soft tissue sarcoma resection
Authors: Lazarides Alexander L. , Saltzman Eliana B. , Visgauss Julia D. , Mithani Suhail K. , Eward William C. , Brigman Brian E. 
Published: January 2022

AbstractFor soft tissue sarcoma patients receiving preoperative radiation therapy, wound complications are common and potentially devastating. The purpose of this study was to assess the feasibility of intraoperative indocyanine green fluorescent angiography (ICGA) as a predictor of wound complications in these patients. A consecutive series of patients with soft tissue sarcoma of the extremities or pelvis who received neoadjuvant radiation and a subsequent radical resection received intraoperative ICGA with the SPY PHI device (Stryker Inc.) at the time of closure. Retrospective analysis of fluorescence signal along multiple points of the wound length was performed and quantified. The primary endpoint was wound complication, defined as delayed wound healing or wound dehiscence, within 3 months of surgery. Fourteen patients with preoperative irradiated soft tissue sarcoma were consecutively imaged. There were six patients with wound complications classified as “aseptic” in five cases. Using the ICGA, blinded surgeons correctly predicted wound complications in 75% of cases. During the inflow phase, a mean ratio of normal of 0.62 maximized the area under the curve (AUC = 0.90) for predicting wound complications with a sensitivity of 100% and specificity of 77.4%. During the peak phase, a mean ratio of normal of 0.55 maximized the AUC (0.95) for predicting wound complications with a sensitivity of 88.9% and a specificity of 100%. Intraoperative use of ICGA may help to predict wound complications in patients undergoing resection of preoperatively irradiated soft tissue sarcomas of the extremities and pelvis.
Biocompatible Composite Microspheres of Chitin/Ordered Mesoporous Carbon CMK3 for Bilirubin Adsorption and Cell Microcarrier Culture
Authors: Yue Pengpeng , Chen Biao , Lv Xiaoyan , Zou Yongkang , Cao Hankun , Ma Yongsheng , Wang Lizhe , Liu Zhongzhong , Zheng Yiran , Duan Bo , Wu Shuangquan , Ye Qifa 
Published: January 2022

AbstractExtra bilirubin in the blood can provoke serious illness in patients with severe liver disease. Hemoperfusion is an effective method to remove the extra bilirubin, but its application is limited by the low adsorption efficiency and poor biocompatibility of available adsorbent materials. In this study, chitin/ordered mesoporous carbon CMK3 (Ch/CMK3) microspheres are successfully prepared. Results of characterization experiments indicated that these composite microspheres possess a multilayered porous nanofibrous structure with an extremely large specific surface area (300.19 m2 g−1) and large pore size. Notably, the Ch/CMK3 microspheres demonstrated a high bilirubin adsorption capacity (228.19 mg g−1) in phosphate buffer solution (PBS), and an outstanding bilirubin removal ratio (76.78% ± 4.40%) in the plasma of rabbits with hyperbilirubinemia without affecting the protein components. More importantly, the Ch/CMK3 microspheres showed no effect on other blood components, no cytotoxicity, and no systemic toxicity to mice. Cell co‐culture experiments revealed that the microspheres can provide a 3‐dimensional (3D) space to promote cell adhesion, proliferation, and nutrient exchange. These Ch/CMK3 microspheres featuring a strong ability for bilirubin adsorption and good biocompatibility can be a promising candidate in biomedical applications such as hemoperfusion, cell microcarrier, and 3D tissue engineering.
Leveraging redundancy in simultaneous multislice acquisitions to improve spike detection
Authors: Sidhu Jagjit Singh , Sakaie Ken , Shin Wanyong , Lowe Mark 
Published: January 2022

PurposeTo improve the performance of low‐level spike noise artifact detection for daily quality assurance protocols by taking advantage of redundancy in simultaneous multislice (SMS) acquisitions.MethodsMagnitude images were transformed into pseudo k‐space images. Time series at each pseudo k‐space point were detrended. A slice was determined to contain spiking artifact if it exceeded an intensity threshold and if all simultaneously acquired slices contained outliers.ResultsA total of 401 112 slices were inspected. Of these, 42 showed a spike artifact, based on visual inspection of image data and k‐space data. With an intensity threshold of 4.6 SDs over time for each pseudo k‐space point, all slices containing artifact were correctly flagged, and only 30 slices were incorrectly flagged when using the SMS criterion. Without the SMS criterion, 12 908 slices were incorrectly flagged as containing artifact. Without the SMS criterion, sensitivity to artifact would have to be sacrificed to substantially reduce the number of incorrectly flagged slices.ConclusionThis study demonstrates that the SMS criterion reduced the number of outliers reported to a manageable level while accurately identifying low‐level spike artifacts. Successfully identifying low‐level spikes allows early detection of hardware problems that can be fixed before the problem becomes debilitating and corrupts data. As part of a daily quality assurance protocol, the method prevents the need to retrospectively carry out time‐intensive despiking and reanalysis of data.
A k‐means clustering machine learning‐based multiscale method for anelastic heterogeneous structures with internal variables
Authors: Benaimeche Mohamed Amine , Yvonnet Julien , Bary Benoit , He Qi‐Chang 
Published: January 2022

AbstractA new machine‐learning based multiscale method, called k‐means FE, is introduced to solve general nonlinear multiscale problems with internal variables and loading history‐dependent behaviors, without use of surrogate models. The macro scale problem is reduced by constructing clusters of Gauss points in a structure which are estimated to be in the same mechanical state. A k‐means clustering—machine learning technique is employed to select the Gauss points based on their strain state and sets of internal variables. Then, for all Gauss points in a cluster, only one micro nonlinear problem is solved, and its response is transferred to all integration points of the cluster in terms of mechanical properties. The solution converges with respect to the number of clusters, which is weakly depends on the number of macro mesh elements. Accelerations of FE calculations up to a factor 50 are observed in typical applications. Arbitrary nonlinear behaviors including internal variables can be considered at the micro level. The method is applied to heterogeneous structures with local quasi‐brittle and elastoplastic behaviors and, in particular, to a nuclear waste package structure subject to internal expansions.
Tetraglycidyl ether 1.1.2.2‐tetraphenyl ethane epoxy resin modified by carbon nanotubes
Authors: Zhou Jun , Lei Yi 
Published: January 2022

AbstractUsually, the reinforcing effect of carbon nanotubes (CNTs) in a polymer is related to the properties of the matrix and its dispersion in the matrix. In this paper, the modification effect of CNTs on high performance tetraglycidyl ether 1.1.2.2‐tetraphenyl ethane epoxy resin (TGETPE) was studied. CNTs grafted with amino groups (N‐CNTs) were used to react with DGEBA to form a well dispersed CNTs / epoxy resin mixture (E‐CNTs), and then E‐CNTs were added to TGETPE/methyl tetrahydrophthalic anhydride (MeTHPA) to form a E‐CNTs‐reinforced TGETPE composites. The effect of different content of E‐CNTs on TGETPE/MeTHPA cured resin was studied. With an increase in content of E‐CNTs, the mechanical properties of the cured sample first increase and then decrease. At E‐CNT contents of 0.4 and 0.6 wt%, the tensile strength and bending strength of the composites increased by 39% and 34%, respectively. Young's modulus of the cured composite increases with the addition of E‐CNTs. In addition, the addition of E‐CNTs can improve the thermal properties of the cured sample.
Regulated acrylate networks as tough photocurable materials for additive manufacturing
Authors: Kury Markus , Ehrmann Katharina , Gorsche Christian , Dorfinger Peter , Koch Thomas , Stampfl Jürgen , Liska Robert 
Published: January 2022

AbstractIn lithography‐based additive manufacturing, mostly crosslinking monomers with acrylate functionality are applied, which yield brittle materials with inhomogeneous network architectures. The toughening of state‐of‐the‐art materials is an integral requirement for the advancement of photopolymer‐based three‐dimensional (3D) products. Here we show that the final material properties of acrylate networks can be adjusted through regulation of the radical curing mechanism using difunctional vinyl sulfonate esters to obtain toughened 3D structured materials. A substantial improvement of the thermomechanical behavior of resulting materials over materials regulated by state‐of‐the‐art reagents (e.g. thiols) is presented and first 3D parts have successfully been printed. Resulting materials exhibit reduced shrinkage stress, reduced warpage, higher overall conversion and higher glass transition temperatures compared with the pure acrylate network. © 2022 The Authors. Polymer International published by John Wiley & Sons Ltd on behalf of Society of Industrial Chemistry.
Leaf coordination between petiole vascular development and water demand in response to elevated CO2 in tomato plants
Authors: Cohen Itay , Lichston Juliana Espada , de Macêdo Cristiane Elizabeth Costa , Rachmilevitch Shimon 
Published: January 2022

AbstractThe rise in atmospheric CO2 has a profound impact on plants physiology and performance. Stomatal gas exchange such as reduction in water loss via transpiration and higher photosynthetic rates are among the key plant physiological traits altered by the increase of CO2. Water acquired in plant roots is transported via the xylem vessels to the shoots. Under conditions of elevated CO2, water flux decreases due to higher water use efficiency and a decline in stomatal conductance. However, the mechanism by which the shoot vascular development is affected under elevated CO2 is still largely unclear in herbaceous crops. In the current study, tomato plants were exposed to either 400 or 800 ppm of CO2 and were analyzed for growth, leaf area, gas exchange rate, and petiole anatomy. Elevated CO2 caused a reduction in metaxylem vessel diameter, which in turn, decreased leaf theatrical conductivity by 400% as compared with plants grown under ambient CO2. This work links anatomical changes in the petioles to the rise in atmospheric CO2 and water use. Plant water demand declined under elevated CO2, while photosynthesis increased. Thus, the decrease in leaf specific conductivity was attributed to lower water consumption in leaf gas exchange and, by extension, to higher leaf water use efficiency. As the global climate changes and water scarcity becomes more common, such anatomical alterations caused by elevated CO2 may affect plant response to water limitation. Further research on petiole anatomical alterations under conditions of combined climate change factors such as drought and heat with elevated CO2 may assist in clarifying the responses expected by future climate scenarios.
Genotypic differences in response of durum wheat (Triticum durum Desf.) to lime‐induced iron chlorosis
Authors: Salhi Khaled , Hajlaoui Hichem , Krouma Abdelmajid 
Published: January 2022

AbstractWheat, durum wheat, is the first cereal cultivated and consumed in Tunisia. Because the dominance of calcareous soils in its agroecological systems, known by their low availability of iron (Fe) inducing Fe chlorosis and limiting crop production, its yield remains low. Therefore, the search for tolerant genotypes is always current. In this context, the physiological behavior of six Tunisian genotypes of durum wheat (salim, karim, razek, khiar, inrat100, and maali) cultivated on calcareous and fertile soils for 2 months in a pot experiment was investigated. A greenhouse was used to conduct experiments under natural light. Plant growth, SPAD index, Fe nutrition, Fe distribution, and photosynthesis were monitored and used to evaluate and discriminate their respective physiological responses. On calcareous soil, results revealed reduced plant growth, active Fe, SPAD index, and net photosynthesis. Genotypic differences in the response of wheat to calcareous‐induced Fe deficiency were observed and allowed to classify the genotypes Salim and Karim as relatively tolerant. These genotypes expressed Fe translocation capacity (FeT) up to 3 times, Fe use efficiency for photosynthesis (FeUEAn) up to 1.6 times, and chlorophyll use efficiency for photosynthesis (ChlUEAn) up to 3.5 times greater than that expressed by the other genotypes, particularly inrat100 and maali. Thus, the relative tolerance of Salim and Karim is the result of the high ability of Fe uptake and translocation to shoots to support chlorophyll biosynthesis, photosynthesis, and plant growth as well as an important Fe and chlorophyll use efficiency.