bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of the effect of protein synthesis inhibitors on growing <i>Escherichia coli</i> bacteria using electrochemical sensors
Authors: Tyulenev A. V. , Smirnova G. V. , Muzyka N. G. , Oktyabrsky O. N. 
Published: December 2022

Background. The study of the mechanisms of action of antibiotics requires the integrated use of traditional microbiological and physicochemical methods. The aim. To study the response of Escherichia coli bacteria to the action of four antibiotics, inhibitors of protein synthesis, using combined approach. Methods. Bacteria were grown under aerobic conditions on minimal M9 medium with glucose. Tetracycline, kanamycin, streptomycin and chloramphenicol have been tested. The effect of antibiotics on survival (CFU) and growth rate was determined. Respiratory activity, sulfide production, extracellular potassium, as well as pH and Eh of the medium were measured using electrochemical sensors directly in a growing culture in the “real time”, membrane potential was measured using a DiBAC dye and a Leica DM2000 fluorescent microscope. Results. The tested antibiotics were divided into two groups according to their properties. Tetracycline and chloramphenicol showed a pronounced bacteriostatic effect, growth inhibition began immediately after the addition of antibiotics and occurred at a high rate. Both antibiotics inhibited respiration, stimulated sulfide production and an Eh jump. Bacteria treated with tetracycline and chloramphenicol retained the ability to maintain membrane potential and intracellular potassium better. Inhibition of respiration led to a decrease in glucose catabolism, as evidenced by a lower rate of acidification of the medium compared to the control. Growth inhibition with streptomycin and kanamycin was initiated with a 30-minute delay. Both antibiotics showed a bactericidal effect, did not stimulate sulfide production and Eh jump, did not inhibit respiration, but caused a drop in membrane potential and intracellular potassium. High respiratory activity promoted glucose catabolism, as evidenced by the rapid acidification of the medium. Of interest is the detection of kanamycin-induced sulfide production during E. coli growth on MOPS medium. Conclusion. An analysis of the data obtained indicates that the use of electrochemical sensors in combination with traditional methods is a promising approach to studying the mechanisms of action of antibiotics.
Metabolic features of naïve and memory CD4<sup>+</sup>T cells in quiescence and during proliferation
Authors: Vlasova V. V. , Saidakova E. V. , Korolevskaya L. B. , Shmagel N. G. , Shmagel K. V. 
Published: December 2022

Background. Memory CD4+ T cells proliferation is the basis for accelerated secondary immune response. The characteristics of memory CD4+ T cells providing their faster division compared to naive CD4+ T lymphocytes are poorly understood. T cells proliferative ability is determined by their metabolism. The metabolic features of proliferating memory CD4+ T cells remain elusive. The aim. To compare the metabolic features of naive and memory CD4+ T cells in quiescence and during proliferation. Methods. Peripheral blood mononuclear cells were analyzed using flow cytometry. Dividing cells were identified by CD71 expression. Cellular glucose and fatty acid uptake was assessed using fluorescent glucose (2-NBDG) and palmitate (BODIPY-FL-C16) analogs, respectively. Glutamine transporter expression was analyzed by staining the cells with anti-ASCT2 antibodies. Mitochondrial mass and membrane potential were measured using MitoTracker Green and MitoTracker Orange, respectively. Results. Quiescent memory CD4+ T cells exhibited elevated levels of glucose and palmitate uptake when compared to naive CD4 + T lymphocytes (p < 0.001). Both subsets had increased substrate consumption when proceeding to proliferation (p < 0.001). When dividing, naive CD4+ T cells consumed more glucose and palmitate than memory CD4+ T cell (p < 0.001). Proliferation caused an increase in mitochondrial mass in naive (p < 0.001) and memory CD4+ T lymphocytes (p < 0.05). In memory CD4+ T cells, unlike naive CD4+ T lymphocytes, an increase in mitochondrial mass wasn’t accompanied by an increase in membrane potential. Conclusion. In memory CD4 + T cells, compared to naive CD4+ T lymphocytes, the metabolic change induced by proliferation is moderate and affects the mitochondrial activity to a lesser extent. Lower bioenergetic expenses of memory CD4+ T cells can contribute to their rapid proliferation during secondary immune response.
Production of immunoregulatory molecules by induced erythroblasts at various stages of cell differentiation
Authors: Nazarov K. V. , Perik-Zavodsky R. Yu. , Kuznetsova M. S. , Philippova Yu. G. , Shevchenko Yu. A. , Denisova V. V. , Silkov A. N. , Sennikov S. V. 
Published: December 2022

Introduction. Bone marrow erythroblasts produce a wide range of cytokines with opposite biological effects. This may be due to a change in the spectrum of production of immunoregulatory mediators during differentiation and small qualitative and quantitative differences in the spectrum of cytokines produced at each stage of differentiation, which may be important for the regulation of hemo- and immunopoiesis. The aim. To study the spectrum of production of mediators by erythroblasts at different stages of differentiation. Methods. Erythroblasts were obtained from CD34+ bone marrow cells of healthy donors in the presence of recombinant cytokines. Phenotype assessment was performed using flow cytometry for erythroid (CD45, CD71, CD235a, CD44) and lymphoid markers (CD3, CD4, CD8, CD16, CD19). Blocking of erythroblast differentiation at different stages was carried out using specific blocking monoclonal antibodies to melanocortin receptors (MCR) of types 1, 2 and 5. Cytokine analysis in conditioned erythroblast media was performed using the Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Bio-Rad Laboratories, USA). Cytokine production was analyzed using the CytokineExplore online tool. Results. The resulting erythroblasts are divided into positive and negative populations according to the CD45 marker, carry markers of erythroid cells CD71, CD235a and do not express linear markers of lymphoid cells. In type 1 MCR blockage, polychromatophilic erythroblasts predominate, in type 2 MCR blockage, basophilic erythroblasts predominate, and in type 5 MCR blockage, orthochromatophilic erythroblasts accumulate. According to the production of cytokines, it was shown that when using any of the blocking antibodies, we obtain cells that differ qualitatively and quantitatively in a number of mediators from the initial population of induced erythroblasts. Conclusion. Thus, we have shown qualitative and quantitative differences in the production of mediators by erythroblasts depending on the stage of differentiation, which can lead to different regulatory effects.
Systematic search for peptide and protein ligands of human serum albumin capable of affecting its interaction with amyloid β peptide
Authors: Loktyushov E. V. , Litus E. A. , Deryusheva E. I. 
Published: December 2022

Background. Human serum albumin (HSA) is a natural buffer of amyloid-β peptide (Aβ), a key factor in the development of Alzheimer’s disease (AD). A promising approach to the AD prevention is to reduce the concentration of free Aβ by targeted stimulation of the interaction between HSA and Aβ. This approach can be implemented by increasing the affinity of HSA to Aβ through the action of HSA ligands, which was previously demonstrated for some low molecular weight ligands. The aim of the study was to search for peptide and protein ligands of human serum albumin capable of affecting its interaction with Aβ. Materials and methods. To perform a systematic search for peptides/proteins, HSA ligands that are capable of affecting Aβ-HSA interaction, we analyzed the DrugBank, BioGRID, and IntAct databases. As criteria for selecting candidates, along with physicochemical characteristics (molecular weight, solubility, blood-brain barrier passage, molar concentration), we used the requirements of extracellular proteins localization and strict association with AD, according to the DisGeNET and Open Targets Platform databases as well as Alzforum online resource. The algorithms for searching and analyzing the obtained data were implemented using the high-level programming language Python. Results. A candidate panel of 11 peptides and 34 proteins was formed. The most promising candidates include 4 peptides (liraglutide, exenatide, semaglutide, insulin detemir) and 4 proteins (S100A8, transferrin, C1 esterase inhibitor, cystatin C). Conclusions. Selected peptide and protein candidates are subject to experimental verification regarding their effect on the HSA-Aβ interaction and can become the basis for the development of first-in-class drugs for the prevention of Alzheimer’s disease.
Uterine fibroid and its association with metabolic syndrome in women of reproductive age (literature review)
Authors: Babaeva N. I. , Belenkaya L. V. , Sholokhov L. F. , Atalyan A. V. , Suturina L. V. 
Published: December 2022

Uterine fibroid is one of the most common gynecological diseases in women of reproductive age and many aspects of this disease remain the subject of investigation. In particular, the role of the metabolic syndrome is of interest as a potential predictor of uterine fibroid or a comorbid condition that has pathogenetic significance. The aim of this literature review is to systematize current data on the prevalence and associations of the metabolic syndrome and uterine fibroid in women of reproductive age. Literature search was carried out using the scientific literature databases eLIBRARY, PubMed, NCBI, CyberLeninka, and the official IDF website for the period from 2010 to 2022. In PubMed search, we used the following terms: uterine fibroid, metabolic syndrome, uterine fibroid and metabolic syndrome, uterine myoma and metabolic syndrome, uterine myoma and metabolic disorders, uterine myoma and MetS. A total of 439 sources were analized, 32 sources met the search criteria. Results. An analysis of the largest epidemiological studies conducted in recent years, both in non-selective populations and in hospital samples, demonstrates a significant prevalence of both uterine fibroid and metabolic syndrome among women. The results presented in the publications which were included in the review indicate that there is a relationship between the presence of uterine fibroid and the manifestations of the metabolic syndrome. Conclusions. Uterine fibroid and metabolic syndrome are mutually aggravating conditions. Women with uterine fibroid have a worse risk profile for cardiovascular disease, and the presence of metabolic syndrome increases the risk of uterine fibroid. One of the ways to reduce the risk of occurrence and growth of uterine fibroid is the timely correction of the metabolic syndrome and its components. On the other hand, the presence of uterine fibroid should be considered as a basis for active detection of metabolic disorders and cardiovascular risks.
Smell and taste disorders in pregnant women with COVID-19
Authors: Kosovtseva A. S. , Bairova T. A. , Rychkova L. V. , Orlova E. A. , Khasnatinov M. A. , Danchinova G. A. , Mikhalevich I. M. , Polyakov V. M. , Cherevikova I. A. , Myasishchev N. A. , Prokhorova Zh. V. , Kolesnikova L. I. 
Published: December 2022

Background. Smell disorder is a recognized clinical symptom of COVID-19 infection. The problem of the loss of sense of smell in the structure of COVID-19 pathogenesis during pregnancy remains unresolved. The aim of the study. To determine the sense of smell and taste in pregnant women with COVID-19, as well as to evaluate the relationship between the sense of smell and the number of SARS-CoV-2 genome equivalents in the nasopharyngeal secretion. Material and methods. In the case-control study, 121 pregnant women participated: 40 of them were infected with COVID-19; 81 were not infected with COVID-19 earlier and at the time of the study. The survey was conducted in July 2021. Sense of smell was assessed using the Connecticut Chemosensory Clinical Research Center (CCCRC) olfactory test, which includes olfactory threshold determination and odor identification assessment. Taste was measured according to the O. Massarelli method. Viral load was calculated using a standardized method for determining the numberof SARS-CoV-2 RNA copies in 1 ml of nasopharyngeal secretion. Results. The incidence of olfactory disorders (moderate / severe and anosmia) in pregnant women with COVID-19 is 62.5 % compared to 22.23 % among pregnant women who have never had COVID-19 (p = 0.002). No significant taste disorders were identified. The threshold value for the manifestation of olfactory disorders was 17794 RNA copies of the SARS-CoV-2 virus, which corresponds to the 37th threshold cycle (Ct). Conclusion. During pregnancy in the acute phase of COVID-19, sense of smell is significantly impaired, but not sense of taste. Decreased sense of smell is associated with SARS-CoV-2 viral load.
PENGARUH PERSEPSI TERHADAP PERILAKU MAKAN PAGI MAHASISWA PROGRAM STUDI PENDIDIKAN KESEJAHTERAAN KELUARGA, JURUSAN PENDIDIKAN TEKNOLOGI DAN KEJURUAN, FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN, UNIVERSITAS SARJANAWIYATA TAMANSISWA YOGYAKARTA
Authors: Andayani Sri Wahyu , Karyaningsih Endang Wani 
Published: December 2022

Tujuan penelitian ini adalah untuk mengetahui gambaran tentang persepsi mahasiswa, perilaku mahasiswa, dan pengaruh persepsi terhadap perilaku makan pagi. Persepsi mahasiswa terhadap perilaku makan pagi merupakan pandangan atau pengamatan seseorang /mahasiswa terhadap suatu obyekÂ yaitu makan pagi. Makan pagi atau sarapan merupakan kegiatan yang dilakukan seseorang di pagi hari sebelum melakukan kegiatan /aktivitas. Hipotesis yang diajukan dalam penelitian ini adalah ada pengaruh positif dan signifikan antara Persepsi mahasiswa terhadap perilaku makan pagi . Penelitian ini termasuk jenis penelitian deskriptif kuantitatif . Populasi penelitian iniÂ adalah seluruh mahasiswa ProdiÂ PKK, JPTK, FKIP, UST yang berjumlah 282 responden. Penelitian ini merupakan penelitian sampel, karena tidak meneliti seluruh populasi, besar sampel dihitung dengan menggunakan rumus sloven yaitu 165 responden. Teknik pengumpulan data menggunakan metode angket . Angket sebelum digunakan diuji validitasnya menggunakan uji korelasi Product Moment Â dan uji reliabilitas menggunakan rumus Alpha Cronbach. Sedangkan teknik analisis data menggunakan teknik analisis deskriptifÂ dan analisis korelasi Product Moment didahului dengan uji persyaratan analisis yaitu uji normalitas dan uji linieritas. Hasil analisis diskriptif menunjukkan bahwaÂ persepsi mahasiswa pada kategori cukup (55,8%), perilaku makan pagiÂ pada kategori cukup (67,9 %). Hasil analisis korelasi Product Moment, diperoleh r hitung = 0,297Â . Nilai r tableÂ pada taraf signifikan 5% untuk N=165 adalah 0,148 ( r xy 0,297 > r table Â 0,148 ). Dapat diinterpretasikan bahwa ada pengaruh positif dan signifikan variable persepsi mahasiswa terhadap perilaku makan pagi. Nilai tersebut didukung dengan harga koefisien determinan sebesar 0,88, artinya besarnya sumbangan persepsi mahasiswa terhadap perilaku makan pagi sebesar 88,0%Â sedangkan sisanya 12,0 %Â dipengaruhi oleh factor lain, seperti keadaan ekonomi, lingkungan keluarga, kesempatan dan sebagainya.Â Kata kunci : persepsi mahasiswa, perilaku makan
Learning through Online Engagement Amidst Covid-19 Pandemic: Exploring Students’ Perceptions, Opportunities and Challenges
Authors: Jyoti Rameshwar 
Published: December 2022

This study aims at exploring how M Ed English (Master of Education) students perceive online learning amidst the COVID-19 pandemic. The study has adopted online collaborative learning theory (OCL) as a theoretical stance for the study and employed a qualitative research design as a research method. Six M Ed students have been purposively selected as research participants, who were learning English in online environment during the COVID-19 pandemic. They were selected from two TU affiliated campuses located in Rupandehi district. In order to collect the data, a semi-structured interview technique has been used. The study uncovers that M Ed English students perceive online learning as an alternative way of learning during pandemic situation. It further depicts that through online learning, students improve their language skills, collaborative practices, and digital literacy. Besides these, the study demonstrates that inadequate skills of ICT in teachers and students, lack of internet and smart digital gadgets, learning anxiety, lack of peace zone at home are some challenges in online learning. The study contributes to the students’ understanding of learning through online mode.
अनुसन्धानात्मक लेखको एपिए ढाँचा र पद्धति
Authors: पौड्याल शालिकराम 
Published: December 2022

अनुसन्धानात्मक लेख अनुसन्धानका निश्चित विधि र पद्धति अवलम्बन गरी लेखिने लेख हो । अनुसन्धानात्मक लेख निश्चित प्राज्ञिक समस्याको प्रामाणिक समाधान पहिल्याउनका निम्ति वस्तुगत तथ्य सङ्कलन गरी ती तथ्यलाई निश्चित सैद्धान्तिक पद्धति अवलम्बन गरी नवीन ज्ञानको खोजी गर्न लेखिन्छ । अनुसन्धानका पद्धतिहरूलाई आधार मानेर लेखिएको लेख वस्तुपरक, तथ्यपरक र वैज्ञानिक मानिन्छ । कुनै पनि विषयका बारेमा खोजबिन गरी गहन र व्यवस्थित ढङ्गबाट अध्ययन गरेर निष्कर्ष दिने काम अनुसन्धानात्मक लेखमा गरिन्छ । प्रस्तुत अनुसन्धानात्मक लेखको आवश्यक तत्वहरू शीर्षक चयन, समस्याको निर्धारण, अनुसन्धानात्मक लेखको उद्देश्य, अनुसन्धानात्मक लेख सङ्गठनको ढाचाका बारेमा प्रकाश पारिएको छ । अनुसन्धानात्मक ढाँचालाई आदि, मध्य र अन्त्यमा विभाजन गरी लेखसार वा सारसङ्क्षेप, शब्दकुञ्जी, विषयपरिचय, अध्ययनविधि, सैद्धान्तिक पर्याधार, छलफल र परिणाम, निष्कर्ष, कृतज्ञताज्ञापन, सन्दर्भ सामग्री, परिशिष्टका बारेमा अध्ययन गरिएको छ । यसका साथै अनुसन्धानात्मक लेखमा प्रयोग हुने शीर्षकीकरण, सन्दर्भाङ्कन, सम्पादन र अभिलेखीकरण, सङ्ख्याको प्रयोग, उद्धरणको प्रयोग, अनुच्छेद योजना आदि पक्षका बारेमा पनि अध्ययन गरिएको छ । यसमा एपिए सातौँ संस्करण (सन् २०२०) ले स्वीकार गरेका ढाँचा र पद्धतिलाई अवलम्बन गरी त्यसका मुख्य पक्षहरूलाई यहाँ प्रस्तुत गरिएको छ ।
PERANCANGAN GAJI KARYAWAN MENGGUNAKAN METODE POINT SYSTEM DI PT. MITRA KARYA TEHNIK INDONESIA
Authors: Daniswara Datu Mas Satriaji , Dewayana Triwulandari Satitidjati , Habyba Anik Nur 
Published: December 2022

PT Mitra Karya Tehnik Indonesia merupakan perusahaan yang belum memiliki divisi sumber daya manusia dan sistem penggajian yang pasti. Penggajian karyawan dilakukan sama rata untuk setiap jabatan dan hal ini menimbulkan ketidakpuasan karyawan terhadap kompensasi yang diterima. Tujuan penelitian ini adalah memberikan usulan kepada perusahaan rancangan gaji karyawan. Berdasarkan metode Point System terdapat tiga tahapan utama yaitu analisa jabatan, evaluasi jabatan, dan penentuan besaran gaji. Metode Pairwise Comparison digunakan dalam penentuan bobot setiap indikator kinerja yang akan digunakan dalam penggajian. Analisa jabatan dilakukan pada lima manajer berbeda divisi yang dirancang ulang sistem penggajiannya. Keterampilan, tanggung jawab, kondisi kerja, dan usaha adalah empat faktor utama yang digunakan dalam evaluasi jabatan. Hasil perhitungan point system didapatkan usulan gaji karyawan yang baru dari nilai satu point dengan nominal Rp 106,596.85 dan alokasi gaji dengan jumlah Rp 24,700,000 dengan Divisi Marketing (Rp 4,937,556); Divisi (Rp 5,033,409); Divisi Purchasing (Rp 4,748,913); Divisi Quality (Rp 4,938,817); dan Divisi Engineer (Rp 5,041,303). Hasil perhitungan ulang gaji menghasilkan keseluruhan gaji yang lebih tinggi untuk masing-masing jabatan jika dibandingkan Upah Minimum Regional (UMR) dan diharapkan dapat meningkatkan loyalitas karyawan.Kata Kunci: Gaji, Analisa Jabatan, Evaluasi Jabatan, Metode Point System
Pendidikan Hukum Tindak Pidana Penganiayaan Dan Aspek Hukumnya Bagi Kader Desa Giriwarno Kec.Kaliangkrik Kab.Magelang
Authors: Kurniaty Yulia , Basri Basri , Krisnan Johny , Hakim Hary Abdul , Nurwati Nurwati 
Published: December 2022

Penganiayaan sering ditempuh sebagai jalan pintas dalam menyelesaikan perselisihan warga, oleh karena itu perlu dilaksanakan pendidikan hukum bagi warga Desa Giriwarno untuk meningkatkan kesadaran hukum melalui penyuluhan hukum dengan tema “Tindak Pidana Penganiayaan Dengan Aspek Hukumnya. Tujuan kegiatan ini adalah untuk meberikan pemahaman keapada masyarakat Giriwarno tentang pentingnya pencegahan terjadinya penganiayaan. Pnegabdian dilaksanakan menggunakan metode ceramah dan diskusi. Hasil dari kegiatan ini warga menjadi paham bahwa tindak pidana penganiayaan terbagi atas kategori ringan, berat dan berencana. Adapun berat ringan sanksi hukumnya mengikuti berat ringannya penderitaan yang dialami korban.
Penyuluhan Hukum di Desa Umbulsari Kecamatan Windusari Tentang Tata Cara Pelaporan Tindak Pidana Kekerasan Dalam Rumah Tangga (KDRT)
Authors: Basri Basri , Kurniaty Yulia , Krisnan Johny , Sulistyaningsih Puji , Nurwati Nurwati 
Published: December 2022

Kehidupan bahagia adalah cita-cita semua orang. Dalam mewujudkan kebahagiaan maka setiap orang bekeluarga dalam rangka membentuk rumah tangga. Ternyata di dalam menjalankan kehidupan berumah tangga tidak selalu berjalan secara tenteram dan damai. Sehingga bahagia yang didambakan tidak terwujud dalam kehidupan berumah tangga. Salah satu persoalan yang sering terjadi dalam kehidupan berumah tanggal adalah kekerasan dalam rumah tangga (KDRT). Di Desa Umbulsari, Kecamatan Windusari tempat penyuluhan hukum dilakukan, berdasarkan observasi dan wawancara yang peneliti lakukan ditemukan sering terjadi tindak kekerasan dalam rumah tangga. Salah satu permasalahan yang dihadapi masyarakat ketika terjadi tindak kekerasan dalam rumah tangga adalah ketidaktahuan tentang tindakan apa yang mesti dilakukan apabila tindak kekerasan itu terjadi. Dari permasalahan ini peneliti berkesimpulan bahwa perlu dilakukan pemberdayaan masyarakat Desa Umbulsari Kecamatan Windusari tentang “Tata Cara Pelaporan dan Pengaduan Tindak Pidana Kekerasan dalam Rumah Tangga (KDRT)”.
Determination of Islamic Month Start by Moonsighting Australia (Case Study: 1 Dzulhijah 1441)
Authors: Fatmawati  , Andi Muhammad Akmal  , Andi Muh. Akhyar  , Azwar  , Achmad Nasyori  
Published: December 2022

The determination of the Islamic calendar is paramount in Islam because it strongly relates to worship, like Ramadan fasting, eid-al-fitr, and zakat fitr. Many studies have examined young moon visibility criteria in many Muslim countries, such as Indonesia, Saudi Arabia, Thailand, and Singapore. However, no study on the initiation of the Islamic month has been conducted in Australia, a Muslim-minority country with middle-eastern immigrants seeking jobs. One of Australia's most trusted organizations to announce the start of Hijri month is Moonsighting Australia. Therefore, this study aimed to analyze the determination of the Islamic calendar by Moonsighting Australia organization based on factors such as method, matla, rukyat time, hilāl visibility, and resistors (1 Dzulhijjah 1441 H). A descriptive study with a qualitative approach used literature reviews, content analysis, and case studies. Primary data were taken from a decision letter from Moonsighting Australia about the start of Dzulhijjah 1441 H, interviews with the coordinator, and relevant references. The findings showed that Moonsighting Australia applies a rukyat method by the naked eye - without any optical aids and hilāl visibility criteria consideration - every 29th of Hijri month. Also, it tunes the concept of matla wilayat al hukmi, where the sighting process and result are implemented throughout Australia’s territory. Keywords: Australia, Dzulhijjah, Hijri, Islamic Month, Moonsighting.