bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNMT3A promotes the proliferation and metastasis of esophageal squamous cell carcinoma via upregulating HDAC9
Authors: Pan Minghong , Feng Yingtong , Ding Peng , Ning Jiayi , Wang Yuanyong , Wang Zhaoyang , Duan Hongtao , Shao Changjian , Jiang Menglong , Ren Xiaoya , Lu Di , Yang Hanyi , Zhou Xin , Zhang Yimeng , Han Jing , Ma Zhiqiang , Yan Xiaolong 
Published: October 2022

AbstractBackground Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide and is characterized by high morbidity and mortality. However, the detailed molecular mechanisms underlying malignant progression of ESCC remain unclear. Methods 140 patients with esophageal squamous cell carcinoma who underwent surgery were enrolled in this study. Clinicopathologic characteristics and Kaplan–Meier survival analysis were performed to investigate DNMT3A expression and HDAC9 expression. Cell functional experiments were implemented both in vivo and in vitro to investigate the effects on ESCC cell proliferation and metastasis. Recombinant lentivirus–meditated gene overexpression or knockdown showed that HDAC9 participated in DNMT3A-mediated ESCC progression in vivo and in vitro, and this was further confirmed by HDAC inhibitor (SAHA) and immunohistochemical. Results We first demonstrated that DNMT3A expression was significantly higher in ESCC tissues than in corresponding adjacent non-cancerous tissues, and high DNMT3A expression was associated with poor prognosis. Then we confirmed that DNMT3A promoted proliferation and metastasis of ESCC cells in vivo and in vitro, suggesting that DNMT3A may be a promising therapeutic target for preventing esophageal cancer. Additionally, we found that DNMT3A up-regulated HDAC9, and HDAC9 overexpression significantly reversed the inhibitory effect caused by DNMT3A gene knockdown. In addition, we further confirmed the involvement of HDAC9 in DNMT3A-mediated carcinogenesis using HDAC inhibitor (SAHA). At last, we also validated HDAC9 expression was significantly higher in ESCC tissues than in corresponding adjacent non-tumour tissues, and high HDAC9 expression was positively associated with poor prognosis. Interestingly, Spearman correlation analysis confirmed a significant positive correlation between DNMT3A and HDAC9 expression. and ESCC patients with simultaneous DNMT3A and HDAC9 expression have the worst prognosis. Conclusions These results suggest that HDAC9 is involved in DNMT3A-mediated progression of ESCC, and targeting DNMT3A and HDAC9 may be a potential ESCC therapeutic strategy.
DBE-KT22: A Knowledge Tracing Dataset Based on Online Student Evaluation
Authors: Abdelrahman Ghodai , Abdelfattah Sherif , Wang Qing , Lin Yu 
Published: October 2022

Abstract
Online education has gained an increasing importance over the last decade for providing affordable high quality education to students worldwide. This has been further magnified during the global pandemic as more students switched to study online. The majority of online education tasks, e.g., course recommendation, exercise recommendation, or automated evaluation, depends on tracking students' knowledge progress. This is known as the Knowledge Tracing problem in the literature. Addressing this problem requires collecting student evaluation data that can reflect their knowledge evolution over time. In this paper, we propose a new knowledge tracing dataset named Database Exercises for Knowledge Tracing (DBE-KT22) that is collected from an online student exercise system in a course taught at the Australian National University in Australia. We discuss the characteristics of the DBE-KT22 dataset and contrast it with the existing datasets in the knowledge tracing literature. Our dataset is available for public access through the Australian Data Archive platform upon proper citation to the authors.
Characterization of Cellulose Nanocrystals (CNCs) Derived from Microcrystalline Cellulose (MCC) Synthesized Using Acid Hydrolysis Method.
Authors: Whba Fathyah , Mohamed Faizal , Idris Mohd Idzat , Yahya Mohd Syukri 
Published: October 2022

Abstract
This paper documented the synthesis of cellulose nanocrystals (CNCs) extracted from commercially-sourced microcrystalline cellulose (MCC) via acid hydrolysis and the subsequent characterization analyses of the yield. The hydrolysis process was completed using 64% sulphuric acid at 45°C for 1 hour. The yield of (CNCs) exhibited prominent physicochemical properties. The X-ray diffraction (XRD) analysis suggested that the crystallinity degree of the CNCs was 8.94% higher than MCC sample. Meanwhile, zeta potential (ζ) analysis showed that the CNCs sample possesses highly negative surface charges of -25.6±8.24 mV. Further investigation by transmission electron microscopy (TEM) analysis revealed the rod-like morphology of the CNCs sample with average diameter of 9.36±3.81nm and 83.84% crystallinity index. Whereas, via field emission scanning electron microscopy (SEM) analysis confirmed considerable reduction in particle size and changes in surface morphology post-acid hydrolysis of the CNCs, with diameter of 7.65±2.56 nm. It is envisaged that the acid hydrolysis technique is promising in synthesizing CNCs of high crystallinity with smaller size for various possible applications, potentially as a contrast agent.
Dynamical modeling and Identification of Li-ion batteries based on Squirrel Search Optimization Algorithm for Electric Vehicle Applications
Authors: DALI Ali , MESBAHI Tedjani , BARTHOLOMEÜS Patrick , Chaaban Khaled 
Published: October 2022

Abstract
This paper presents an advanced method for modeling and parameter identification of the lithium-ion (Li-ion) battery using an experimental characterization. A comparison study between two Li-ion models and a proposed one are performed where each model parameters are identified using three different algorithms. The obtained models are then tested and validated using experimental data obtained from a real test bench. The identification methods are implemented using a precise nonlinear model based on electric equivalent circuit of Li-ion battery and the parameter identification process formulated as a nonlinear optimization problem. In order to compare the capability of each model to represent effectively the Li-ion battery regardless of the optimization method, three optimization algorithms are involved: Squirrel Search Algorithm (SS), Particle Swarm Optimization (PSO) and Genetic Algorithm (GA). Numerical simulations along with experimental validation are performed on 20 Ah Li-ion battery. The models are compared according to the lowest fitness function levels of each algorithm and their limitation were also discussed in this paper. The obtained results show that the proposed models are able to simulate the dynamic behavior of Li-ion battery with good performances.
SPOCD1 serves as a prognostic marker in colon cancer and is associated with immune infiltration
Authors: Hui Juan , Liu Hao , An Guangzhou , Zhou Yun , Liang Junrong , He Yangsong , Wang Pei , Wang Boda , Wang Xin , Lu Yuanyuan , Zhao Xiaodi 
Published: October 2022

Abstract
Purpose Colon cancer is among the most common malignant tumors with poor prognosis in the world. SPOCD1 encodes transcription factor S-II family proteins whose aberrant expression is linked to tumor progression and immune regulation. However, it remains unclear whether SPOCD1 contributes to the progression and immunoregulation of colon cancer. Therefore, we sought to explore and confirm the expression of SPOCD1 in colon cancer and its association with immune regulation in this study.
Methods Based on RNA-seq data from the TCGA colon cancer project, we explored the expression of SPOCD1 and its relationship with the OS, DSS, and PFI of patients with colon cancer. Moreover, the connection between SPOCD1 and the infiltration level of immune cells, immunomodulators and chemokines and receptors in colon cancer was comprehensively investigated utilizing the TIMER, GEPIA and TISIDB databases. Finally, we confirmed the expression of SPOCD1 in colon cancer and its correlation with immune cell infiltration by immunohistochemistry and multiplex immunohistochemistry.
Results We found that SPOCD1 expression was increased in colon cancer and had tremendously diagnostic and prognostic value. The area under the curve (AUC) for SPOCD1 in COAD is 0.910 (95% CI 0.875–0.946) and high expression of SPOCD1 indicates a shorter OS, DSS and PFI in patients with colon cancer. Additionally, the increased expression of SPOCD1 in colon cancer is linked to the infiltration levels of immunomodulators, chemokines and receptors, neutrophils, macrophages and cancer-associated fibroblasts. We demonstrated that the expression of SPOCD1 was elevated in colon cancer tissues and expressed in both the nucleus and cytoplasm by immunohistochemistry. Through a multiplex immunohistochemistry experiment, we further confirmed the positive correlation between SPOCD1 expression and the expression of immune markers, including CD11b, CD163 and αSMA.
Conclusion Taken together, our results suggest that SPOCD1 can be utilized as a promising indicator for diagnosis and prognosis evaluation and has tremendous potential to modulate the immune microenvironment in the progression of colon cancer.
The impact of climate change on Quaternary glaciers of Gharaghom Basin in Iran
Authors: Azirani Tayebeh Akbari , Ghorbani Hossein 
Published: October 2022

Abstract
This research has mapped the quaternary equilibrium line altitude (ELA) using Whrite’s and Porter’s methods to reconstruct past and present temperatures using a regression model and to detect the potential impact of climate change on glaciers using an artificial neural network in the Gharaghom basin located in northeastern Iran. Defining a regression model between temperature and altitude based on available observation data and reconstructed data indicated that the present temperature lapse rate of -0.41°C would occur for every hundred meters of increasing altitude in stations. The reconstruction of the past temperature of the Pleistocene revealed an increase of about 10.36–10.5 ˚C for the mean annual temperature of the current temperature in the basin. The recent ELA rises from the Pleistocene 2236 m and 2200 m a.s.l according to Whrite and Porter’s methods respectively. So, considering the present temperature lapse rate and the highest altitude of 3271m in the basin, the maximum Pleistocene ELA depression would be about 800m due to modern temperature increase of 10.36–10.5°C compared to the past Pleistocene temperature. Based on the neural network prediction model, linear growth in temperature would occur for the next three decades (2021–2051) in the Gharaghom basin. Based on trend analysis, the minimum temperature has been much more affected than the maximum temperature, so global warming has caused an increase in the monthly minimum and maximum temperature in most seasons in different parts of the Basin. The average temperature rise of 0.19°C for future decades would consequently affect the water resources in the basin.
Energy System Analysis with a Focus on Future Energy Demand Projections: the case of Norway
Authors: Malka Lorenc , Bidaj Flamur , Jaku Aldona , Roçi Rexhina , Gebremedhin Alemayehu , Kuriqi Alban 
Published: October 2022

Abstract
Post Covid-19 pandemic and the Ukrainian war is having a significant impact on energy systems worldwide, faltering investments and threatening to throttle the expansion of primary clean energy technologies even in the case of a well-structured and managed energy system, such as Norway. This unprecedented crisis requires deeper analyses of different national energy systems. Hence, providing and highlighting needed interventions and improvements in the actual energy system in the case of Norway is crucial. The focus of this study is to analyze demand-side in households, industry, and transport sectors. LEAP model, a powerful demand-side energy system analysis tool, was used to conduct the analysis. The energy demand projections for 2050 are estimated firstly by considering a population growth rate of 0.8%. Secondly, Norway has set itself an ambitious target of decreasing GHG emissions in 2030 by 55% compared to 1990 levels and 90–95% by the year 2050. It aims the diversification of the overall national energy system. From the perspective of climate change mitigation, EVs include an attractive option, other sustainable fuel sources such as H2, biofuel mixed with diesel, the use of excess heat to cover households' heating demand supplied by industry, and integration of large-scale heat pumps driven by RES during off-peak demand is applied. Energy demand projections are uncertain, and the main goal is to show how different scenario projections up to 2050 affect the energy system of Norway, showing that the combined global warming potential (GWP) will be around 28.9 million metric CO2 from 66 million metric CO2 tones released in the current account scenario.
In-Hospital Outcomes and Biomarkers of Acute Myocardial Infarction With Concomitant Bacterial infection：A Retrospective Study
Authors: Qian Lu , Zhang Suqin , Lin Jiafeng , Li Zhuoyuan 
Published: October 2022

Abstract
Background
As a trigger of acute myocardial infarction (AMI), bacterial infection usually accompanies with AMI, and will lead to worse outcomes of AMI patients. We aimed to assess the prognosis of AMI with concomitant bacterial infection and find out the best laboratory examinations to recognize it.
Methods
All patients hospitalized for an AMI in cardiology department were prospectively included. Patients were stratified into those with or without concomitant bacterial infection. Outcomes and laboratory examinations were compared between groups in unadjusted and adjusted analyses.
Results
Among the 456 patients hospitalized for AMI, 120 (26%) had a concomitant diagnosis of bacterial infection. Out-comes in hospital were worse in patients with bacterial infection (more acute heart failure: 61.7% vs. 22.6%, p < 0.001, and higher all-cause mortality in hospital: 15% vs. 3.9%, p < 0.001). In the Receiver Operating Curves (ROC) of biomarkers of AMI and concomitant bacterial infection, Areas under the Receiver Operating Curves (AUC) for c-reaction protein (CRP) and CRP to Platelet Ratio (CRP/PLA) were higher than Neutrophil to Lymphocyte Ratio (NLR) and leucocyte count. (0.852(0.81–0.89) mg/L, 0.848(0.81–0.89) *10− 9mg, p < 0.001). The sensitivity of CRP and the Specificity of CRP/PLA were the highest (80% and 88%). After adjusting for confounders, CRP/PLA (> 0.08) was associated with a fivefold increased risk of bacterial infection when compared with other biomarkers (OR (95%CI) = 5.62 (2.64–11.96), p < 0.001). CRP (> 8.05) was also associated with a higher risk of bacterial infection (OR (95%CI) = 4.02 (1.81–5.85), p = 0.001).
Conclusions
Bacterial infection will lead to worse outcomes of AMI patients, including in-hospital mortality and heart failure. It is the first time to use the CRP/PLA to distinguish AMI with concomitant bacterial infection from other AMI patients. The combination of CRP > 8.05 mg/L and CRP/PLA > 0.08*10− 9mg was the best hallmark of AMI with concomitant bacterial infection.
Expression of Src kinase in hypertrophic cardiomyocytes of the left ventricle of exercising rats
Authors: Liu Mengmeng , Li Pengge , Li Lijin , Sun Mengjiao , Hou Suyun , Li Zhen , Sun Xinxin , Hua Shaohua 
Published: October 2022

Abstract
Objective: To identify the downstream target protein Src kinase of the SDF-1/CXCR-4 pathway in exercise rat hypertrophic cardiomyocytes.
Methods: The cardiomyocytes of 14 successfully modeled swimming rats were used as the control group, and the CXCR-4 inhibitor AMD3100 was applied to the cardiomyocytes of swimming rats for 30 min as the experimental group. The expression levels of Src kinase in the cardiomyocytes of both groups were measured using immunoblotting assay (westernblot, WB).
Results: Compared with the control group, the expression of Src kinase was significantly lower in the experimental group (P < 0.05).
Conclusion: The downstream target protein of the SDF-1/CXCR-4 pathway in exercising rat cardiomyocytes is Src kinase, which is involved in the remodeling process of the left ventricle in exercising rats.
Analysis of the differences in water chemistry characteristics and spatial-temporal variation patterns in typical areas in northern and southern Xinjiang – a case study in Fukang City and Cele County
Authors: 马 玉 , Liu Xiaolong , Bai Xiang 
Published: October 2022

Abstract
Water chemistry characterization of natural water bodies is important for the study of rational water resources utilization in arid zones. In this paper, the hydrochemical characteristics of natural water bodies in the south (Cele) and north (Fukang) parts of Xinjiang, China, during 2006-2016 were analysed. It was found that the ion content of surface water in Cele in South Xinjiang was higher than that in Fukang in North Xinjiang, while the opposite was true for groundwater ion content; there was a good correlation between TDS and each ion content, and the mineralisation of natural water bodies was controlled by Na+ and SO42-; the water chemistry types in Cele were Na+-Cl- and mixed, and those in Fukang were Ca2+-HCO3-, Na+-Cl- and mixed; the water bodies are mainly influenced by the control of rock weathering, with the groundwater in Fukang being controlled by evaporative crystallisation; the water bodies in Cele are mostly suitable for irrigation water, and the surface water in Fukang is more suitable for irrigation water compared to groundwater.
Silver nanoparticle toxicity on Artemia parthenogenetica nauplii hatched on axenic tryptic soy agar solid medium
Authors: Do Minh Anh , Pham Hong Lam Thi , Tran Tuyet Anh , Le Van Cam Thi , Young Tim , Le Dung Viet , Dang Hoa Thi , Doan Nhinh Thi 
Published: October 2022

Abstract
The use of gnobiotic brine shrimp (Artemia spp.) for ecotoxicology and bacteria-host interaction studies is common. However, requirements for axenic culture and matrix effects of seawater media can be an obstacle. Thus, we investigated the hatching ability of Artemia cysts on a novel sterile Tryptic Soy Agar (TSA) medium. We further optimized the culture conditions for temperature and salinity and assessed this culture system for toxicity screening of silver nanoparticles (AgNPs) across multiple biological endpoints. Maximal hatching of embryos occurred at 28°C and without addition of sodium chloride. When capsulated cysts were cultured on TSA solid medium Artemia were negatively impacted by AgNPs at 30–50 mgL-1 in terms of embryo hatching rate, umbrella- to nauplii-stage transformation rate, and nauplii-stage growth. At 50–100 mgL-1 AgNPs and higher, evidence of damage to lysosomal storage was recorded. At 500 mgL-1 AgNPs, development of the eye was inhibited and locomotory behavior impeded. Our study reveals that Artemia cysts can hatch on TSA solid medium which offers more controllability of axenic requirements.
High levels of m6A methylation and histone acetylation modification patterns contribute to the survival of LUAD patients
Authors: Jia LiGuo , Gao ZhenZhen , Chen Jing 
Published: October 2022

Abstract
Background
The m6A methylation-regulated histone acetylation modification affects the proliferation and differentiation of mouse embryonic neural stem cells, and recent studies have shown that the deacetylase SIRT1 regulates RNA m6A methylation to promote hepatocellular carcinogenesis. However, the interrelationship between the m6A methylation and histone acetylation, and the potential roles as well as interactions of related regulators in TME cell infiltration and drug sensitivity have not been explored at a holistic level.
Methods
Unsupervised clustering method was used to identify lung adenocarcinoma m6A modification patterns based on 14 m6A regulators and histone acetylation modification patterns based on 37 histone acetylation regulators. Individual samples were then quantified based on their differential gene construction models. Finally, the models were analysed in relation to transcriptional background and TME characteristics to predict potential target drugs and explore the association of m6A methylation with histone acetylation.
Result
Three histone acetylation patterns as well as two m6A methylation patterns were identified. Patients with LUAD in the low-score group had poorer overall survival times and more active cancer-related malignant pathways. m6A methylation was strongly associated with histone acetylation, and high levels of m6A methylation and histone acetylation patients had significantly superior survival and immunoreactivity. VX-680 and GW843682X may be potential drugs available for the low level m6A group.
Conclusion
This work revealed that m6A modifications and histone acetylation modifications have a non-negligible role in the development of TME diversity and complexity. We found that both m6A methylation and histone acetylation are closely associated with tumor malignant pathways. Combined m6A methylation and histone acetylation analysis will help to obtain the understanding of tumor internal regulation and provide new therapeutic directions.
Exergy and Energy Analysis for a Feasibility Trigeneration System at Kocaeli University Umuttepe Campus
Authors: KADIOĞLU Ebru KOLAY , ALTINAY Meral 
Published: October 2022

Abstract

In this study, a feasibility of the trigeneration system, which is one of the on-site energy production methods, was prepared for Kocaeli University Umuttepe Campus, and energy and exergy analyses were made for each point of the system and exergy destruction in the lines was found. It is aimed to be able to produce the energy, which is the most basic indicator of sustainability, at the closest point to the consumption place, thus keeping the distribution and transmission losses at a minimum level and dimensioning the designed system. The key point of the gains, losses and efficiency analyses in the lines is presented with engineering calculations based on thermodynamic proofs, exergetic efficiency and losses, the energy and balance equations of the whole system, and the feasibility of the entire trigeneration system, including mathematical modeling, energy analysis, the whole system in II. Law Efficiency, COP, Thermal efficiency were calculated. The seasonal working methods were shown by the numerical analyses prepared in the summer and winter working disciplines of the system effectively and the seasonal working methods were found and the working conditions and operating load values ​​suitable for the current season of the system were found. In addition, by performing separate mathematical models for summer and winter mode, in the light of the above-mentioned technical studies, both the reduction in the energy production costs of the country thanks to the energy that the university will produce in-house, the reduction of foreign dependency in energy demand, a feasibility report ready for investors and an academic. In this sense, it is aimed to present a ready-to-use design of a medium-sized power plant that will set an example for trigeneration studies. From the designed system; 66% exergic efficiency in winter, thermal efficiency 0.87411 with 2.05 MW thermal capacity, 63% exergic efficiency in summer, COP 0.82 and 1.5 MW cooling capacity, and 2.02 MWe instantaneous electrical power was found in summer and winter. The results have shown us that the designed system can be a facility that is open to development and worth establishing with the results obtained technically. Based on the current applications made so far, it has been determined that there are similar results between the studies in the literature and the exergy and energy analyses of the trigeneration system, which we have made feasibility in Umuttepe campus and are close to the findings obtained in the calculations in the literature.