bluuebunny/crossref_metadata_2025_split huggingface dataset:
High levels of chromosomal instability facilitate the tumor growth and sphere formation
Authors: Iemura Kenji , Anzawa Hayato , Funayama Ryo , Iwakami Runa , Nakayama Keiko , Kinoshita Kengo , Tanaka Kozo 
Published: June 2022

AbstractMost cancer cells show chromosomal instability (CIN), a condition in which chromosome missegregation occurs at high rates. Growing evidence suggests that CIN is not just a consequence of, but a driving force for, oncogenic transformation, although the relationship between CIN and tumorigenesis has not been fully elucidated. Here we found that conventional two‐dimensional (2D) culture of HeLa cells, a cervical cancer‐derived cell line, was a heterogenous population containing cells with different CIN levels. Although cells with high‐CIN levels (high‐CIN cells) grew more slowly compared with cells with low‐CIN levels (low‐CIN cells) in 2D monolayer culture, they formed tumors in nude mice and larger spheres in three‐dimensional (3D) culture, which was more representative of the in vivo environment. The duration of mitosis was longer in high‐CIN cells, reflecting their higher mitotic defects. Single‐cell genome sequencing revealed that high‐CIN cells exhibited a higher karyotype heterogeneity compared with low‐CIN cells. Intriguingly, the karyotype heterogeneity was reduced in the spheres formed by high‐CIN cells, suggesting that cells with growth advantages were selected, although genomic copy number changes specific for spheres were not identified. When we examined gene expression profiles, genes related to the K‐ras signaling were upregulated, while those related to the unfolded protein response were downregulated in high‐CIN cells in 3D culture compared with 2D culture, suggesting the relevance of these genes for their survival. Our data suggested that, although CIN is disadvantageous in monolayer culture, it promotes the selection of cells with growth advantages under in vivo environments, which may lead to tumorigenesis.
Multi‐system factors impacting youth justice involvement of children in residential out‐of‐home care
Authors: Baidawi Susan , Ball Rubini 
Published: June 2022

AbstractChildren placed in residential care are significantly over‐represented in youth justice systems. Drawing on interviews and focus groups with service providers, this exploratory study examines practice factors that impact on the criminalization of this group of children across multiple services and systems, including in the residential care environment, police, lawyers, courts and youth justice systems, as well as multi‐systems practice with this group in one Australian state. Positive outcomes were observed for children in residential care where well‐functioning care teams existed, as well as for children in therapeutic residential care settings. However, clear limitations were identified across all phases of children's youth justice system involvement, including placement with offending peers, the criminalization of behaviours of concern, greater use of remand and detention, limited support to navigate legal and youth justice processes, challenges to service collaboration, and limited applicability of sentencing considerations. The findings indicate a pervasive level of systemic disadvantage for this group of children, and imply that a holistic strategy underpinned by affirmative action across several systems will be necessary to address the ongoing criminalization of children in residential care.
Impact of the novel CYP2C:TG haplotype and CYP2B6 variants on sertraline exposure in a large patient population
Authors: Bråten Line Skute , Ingelman‐Sundberg Magnus , Jukic Marin M. , Molden Espen , Kringen Marianne Kristiansen 
Published: June 2022

AbstractSertraline is a commonly used SSRI antidepressant drug, metabolized by CYP2C19 and CYP2B6, that exhibits a substantial interindividual variation in clinical response, of which only a part can be attributed to known genetic variants. In the current study we have examined the role of a newly discovered ultrarapid CYP2C:TG haplotype and CYP2B6 variants in order to identify the possible missing heritability for such variation in sertraline response in a large patient population (n = 840). Compared to the reference group (CYP2C19*1/*1, n = 160), sertraline exposure was increased by 128% in CYP2C19 PMs (n = 29, p < 0.001) but decreased by about 20% in CYP2C19 ultrarapid metabolizers (Ums) (homozygous carriers of CYP2C19*17 and/or CYP2C:TG haplotype) with the diplotypes CYP2C19*17/*17, CYP2C:TG/TG, or CYP2C19*17/CYP2C:TG (n = 135, p < 0.003, p = 0.022, p < 0.003, respectively). Interestingly, in patients carrying the increased function CYP2B6*4 allele, and also carrying the CYP2C19*17 and CYP2C:TG alleles (n = 10), sertraline exposure was 35.4% lower compared to the reference group, whereas in subjects being poor metabolizers (PM) in both the CYP2C19 and CYP2B6 gene, the sertraline concentrations were raised by 189%. In summary, the CYP2C19 variants including the CYP2C:TG haplotype had a significant impact on sertraline metabolism, as well as the CYP2B6*4, *6, and *9 alleles. Knowing the CYP2B6 and CYP2C19 genotype, including the CYP2C:TG haplotype status, can prospectively be useful to clinicians in making more appropriate sertraline dosing decisions
Hypoglycaemia and rebound hyperglycaemia increase left ventricular systolic function in patients with type 1 diabetes
Authors: Andreasen Christine R. , Andersen Andreas , Hagelqvist Per G. , Lauritsen Julius V. , Jørgensen Peter G. , Engberg Susanne , Faber Jens , Hartmann Bolette , Pedersen‐Bjergaard Ulrik , Knop Filip K. , Vilsbøll Tina 
Published: June 2022

AbstractAimTo investigate echocardiographic changes during acute hypoglycaemia followed by recovery to hyperglycaemia or euglycaemia in patients with type 1 diabetes.Materials and MethodsIn a randomized crossover study, 24 patients with type 1 diabetes took part in two experimental study days, consisting of a hyperinsulinaemic‐euglycaemic phase (5.0‐8.0 mmol/L) for 45 minutes followed by a hyperinsulinemic‐hypoglycaemic phase (2.5 mmol/L) for 60 minutes, and a recovery phase in either hyperglycaemia (20 mmol/L) or euglycaemia (5.0‐8.0 mmol/L) for 60 minutes. Cardiac function was evaluated with echocardiography during each phase.ResultsAcute hypoglycaemia increased all markers of left ventricular (LV) systolic function, including LV ejection fraction (LVEF), global longitudinal strain (GLS), GLS rate and peak systolic velocity of mitral annular longitudinal movement (s'; P < 0.001 for all). During the recovery phases, all markers of LV systolic function were increased during hyperglycaemia (P < 0.01 for all), and LVEF and GLS remained increased during euglycaemia (P = 0.0116 and P = 0.0092, respectively). The increment in LVEF during the recovery phase was greater during hyperglycaemia than euglycaemia (P = 0.0046).ConclusionsHypoglycaemia, recent hypoglycaemia, and overcorrection of hypoglycaemia to rebound hyperglycaemia increased LV systolic function in type 1 diabetes and may imply consideration of plasma glucose when evaluating LV function in patients with type 1 diabetes. An increase in LV systolic function may cause increased strain on the heart and partly explain the link between hypoglycaemia, high glycaemic variability and cardiovascular disease.
Multiple Lines of Evidence for Estimating NSZD Rates Overlying a Shallow LNAPL Source Zone
Authors: Wozney Anne , Hers Ian , Stevenson Krista , Campbell Calista , Nickerson Nick , Gosse Colleen 
Published: June 2022

AbstractQuantitative methods of monitoring natural source zone depletion (NSZD) via biodegradation of petroleum hydrocarbons (PHC) are required to investigate source zone longevity and guide long‐term management of PHC impacted sites. Vadose zone NSZD processes can be monitored using analysis of surficial CO2 effluxes, soil‐gas gradients, and thermal gradients. This study describes an applied research and development program conducted at a former refinery site over a 4‐year period (2015 to 2019) on quantitative technologies for evaluation of NSZD of PHC light nonaqueous phase liquid (LNAPL) present within a shallow soil zone. A multiyear study using discrete CO2 efflux measurements from dynamic closed chambers was compared with estimates obtained using static traps and continuous monitoring using forced diffusion (FD) technology. Thermistor strings along a transect were used to monitor hourly thermal gradients and assess NSZD rates using the temperature gradient method. Discrete soil‐gas data were used to quantify the vertical oxygen gradient to estimate NSZD rates using the concentration gradient method (CGM). Results of discrete and continuous monitoring methods provide estimates of monthly NSZD rates that range seasonally from 80 to 1300 US gal/acre/year using radiocarbon corrected CO2 efflux method for decane (C10H22) equivalent, 120 to 1600 US gal/acre/year using CGM (for wet to dry conditions) and 400 to 2000 US gal/acre/year using the temperature gradient method. Both seasonal temperature and precipitation fluctuations contribute to variability in rates. Continuous methods are shown to provide for improved resolution of temporal variability and seasonal estimates, although discrete methods provide for improved spatial quantification.
Shade tree isolation in pastures modulates diversity of epiphyte‐dwelling spiders: The role of epiphyte biomass and species dispersal capacity
Authors: Quijano‐Cuervo Luis G. , Rao Dinesh , Escobar Sarria Federico , Dáttilo Wesley , Negrete‐Yankelevich Simoneta 
Published: June 2022

Abstract
In Mexico, most forest habitats have been lost, leaving mostly agroecosystems such as coffee plantations. In some areas, coffee plants are commonly planted under a closed canopy of shade trees and sometimes these coffee plantations are transformed into cattle pastures, in which scattered and isolated trees remain.
Isolated trees (i.e. trees without contact between their crowns) left in disturbed environments such as pastures represent an extreme of habitat reduction for tree‐dwelling organisms. However, these isolated trees can also be biodiversity reservoirs, serving as regeneration nuclei and connection points between terrestrial habitats.
In this study, we evaluated whether the alpha and beta diversities of epiphyte‐dwelling spiders differ between isolated trees in a pasture and shade trees (i.e. which sustain a continuous canopy above the crop) in a coffee plantation, and whether epiphyte biomass and differences in the dispersal capacity of the spider species could explain these changes.
We found that spider alpha diversity was almost 50% higher in isolated trees than in shade trees. Isolated trees had the highest epiphyte biomass and there was a positive relationship between spider diversity and epiphyte biomass in these trees but not in shade trees.
Spider community composition differed between tree conditions. We found that compositional similarity decreased and turnover increased with distance between trees only for spiders with low dispersal capacity in isolated trees.
The high subset of unique species and high species richness in isolated trees highlight their importance in preserving spider communities in agrolandscapes.
Pre‐processed fruits as raw materials: part II—process conditions, demand and safety aspects
Authors: Bhatkar Nikita S. , Shirkole Shivanand S. , Brennan Charles , Thorat Bhaskar N. 
Published: June 2022

SummaryThe demand for pre‐processed fruits has grown rapidly in recent years as a result of consumer attention towards fresh, healthy and convenient foods. Most of the fruits are available seasonally and they are perishable, so it is necessary to have a continuous supply of the fruits to avoid the blockages in the fruit processing industry. The review is written in the purview of this issue, and it presents pre‐processed fruits as a sustainable solution. The present review is part II of the series of articles on pre‐processed fruits as the raw material for various food industries. The fruits covered here are mango, pineapple, pear, olive and papaya. The article continues to emphasise the critical processing and storage condition for these fruits ranked higher in terms of their production quantity and economic value. The fruits are critically reviewed for their processing, storage and safety aspects along with key research findings of different processing techniques. The important safety aspects associated with such processed commodities are also discussed.
Cold sintering co‐firing of (Ca,Bi)(Mo,V)O4–PTFE composites in a single step
Authors: Li Yongqiang , Zheng Mupeng , Zang Moyan , Zhu Mankang , Hou Yudong 
Published: June 2022

AbstractBecause of large differences in the processing temperature windows between ceramics and polymers, the single‐step co‐sintering of microwave dielectric ceramic–polymer substrates remains challenging. In this work, a dense (Ca0.65Bi0.35)(Mo0.65V0.35)O4 (CBMVO) ceramic was first prepared through cold sintering at 150°C, under a uniaxial pressure of 300 MPa for 60 min with Li2MoO4 (LMO) as a transient low‐temperature solvent. Cold‐sintered CBMVO–5 wt% LMO ceramic shows excellent microwave dielectric properties: εr ∼ 11.4, Q × f ∼ 7070 GHz, τf ∼ −7.4 ppm/°C. Moreover, the optimized cold sintering process enabled the preparation of a layered co‐sintered (2–2 type) CBMVO–polytetrafluoroethylene composite, which maintained excellent microwave dielectric properties and showed a good heterogeneous interface bonding. The proposed cold sintering co‐firing of ceramic–polymer composites in a single step shows great potential for application in the seamless integration between ceramics and polymer substrates.
Continuous glucose monitoring system profile of women diagnosed as gestational diabetes mellitus by International Association of Diabetes and Pregnancy Study Groups criteria and labeled as normoglycemic by alternate criteria in early pregnancy
Authors: Gupta Yashdeep , Singh Charandeep , Goyal Alpesh , Mani Kalaivani , Bharti Juhi , Singhal Seema , Kachhawa Garima , Kulshrestha Vidushi , Kumari Rajesh , Mahey Reeta , Sharma Jai B , Malhotra Neena , Bhatla Neerja , Khadgawat Rajesh , Tandon Nikhil 
Published: June 2022

AbstractAims/IntroductionWe aimed to evaluate and compare continuous glucose monitoring system (CGMS)‐based glycemic parameters in women in early pregnancy (<20 weeks of gestation) who were classified as: (i) gestational diabetes mellitus (GDM) by the International Association of Diabetes and Pregnancy Study Groups (IADPSG), but normoglycemia by alternate (UK National Institute for Health and Care Excellence, Canadian Diabetes Association and Diabetes in Pregnancy Study group of India) criteria; and (ii) normoglycemia by both (IADPSG and alternate) criteria.Material and MethodsIn this cross‐sectional study, eligible women underwent standard 75‐g oral glucose tolerance test, followed by the placement of a CGMS. Glycemia‐related parameters were calculated using the standard approach for CGMS data in pregnancy.ResultsWe enrolled 96 women at 14.0 ± 3.2 weeks of gestation. Of the women diagnosed as GDM by IADPSG criteria, 34.2%, 26.3% and 44.7% were classified as normoglycemic by UK National Institute for Health and Care Excellence, Canadian Diabetes Association and Diabetes in Pregnancy Study group of India criteria, respectively. Mean 1‐h postprandial glucose and time above range were significantly higher in women who were GDM by IADPSG, but normoglycemia by Canadian Diabetes Association criteria, compared with women with normoglycemia using both criteria. Similarly, mean 1‐h postprandial glucose, 2‐h postprandial glucose, peak postprandial glucose, 1‐h postprandial glucose excursion and time above range were significantly higher in women who were not identified as GDM by the UK National Institute for Health and Care Excellence criteria. Finally, women missed by the Diabetes in Pregnancy Study group of India criteria had significantly higher mean 1‐h postprandial glucose, 2‐h postprandial glucose, peak postprandial glucose, postprandial glucose excursion, 24‐h glucose and time above range parameters.ConclusionsMore than one‐quarter of women diagnosed as GDM by IADPSG criteria are not identified by alternate criteria. Such women are significantly different from normoglycemic women in terms of several CGMS‐based glycemic parameters of clinical significance.
Lethal effect of ethanol on Solenopsis invicta Buren and economic fish
Authors: Song Shicheng , Liang Mingrong , Wang Lei , Xu Yijuan 
Published: June 2022

AbstractThe red imported fire ant (RIFA), Solenopsis invicta Buren, is an invasive pest species worldwide in many countries and regions. At present, its control mainly depends on synthetic insecticides. Although synthetic insecticides have the advantages of efficacy and convenience, their widespread use has a negative impact on the diversity of local arthropods. Ethanol is widely used as a disinfectant and has been consumed by people, but its insecticidal effect has not yet been evaluated. Here, the control effect of ethanol on the RIFA and its toxicity on four fish species were evaluated. The results exhibited that mortalities of all tested adult castes reached 100% after soaking in 70% v/v ethanol solution for one hour. All reproductive and worker pupae survived at 60% v/v ethanol treatment. In the soaking plus fumigation experiment, all adult castes and brood except female pupae were sensitive to 30% v/v ethanol solution. Conversely, female pupae showed the highest resistance to ethanol. More than 60% v/v ethanol concentration was needed to cause its 100% mortality. In the field experiment, 25% v/v and 50% v/v ethanol solution resulted in 70% and 80% reduction of mound 14 d after drench treatment, respectively. The ethanol vapour in the nest soil was higher than 80 ppm after 72 h of mound drench in the field with 25% v/v ethanol solution. Moreover, 30% v/v ethanol solution did not adversely affect the survival of four fish species exposed to the solution for a short time. This study suggests that mound drench can be alternatively employed with ethanol as a green organic solvent to control RIFA.
Comparison between connective tissue graft and xenogeneic acellular dermal matrix to treat single gingival recession: A data reanalysis of randomized clinical trials
Authors: Miguel Manuela Maria Viana , Ferraz Laís Fernanda Ferreira , Rossato Amanda , Cintra Tuana Mendonça Faria , Mathias‐Santamaria Ingrid Fernandes , Santamaria Mauro Pedrine 
Published: June 2022

AbstractObjectivesThere are few studies comparing xenogeneic acellular dermal matrixes (XDMs) with connective tissue graft (CTG) to treat single gingival recession (GR). The present study involved a reanalysis of previous studies and comparison of CTG or XDM to treat single RT1/GR.Materials and methodsFifty patients from three previous randomized clinical trials treated either by CTG (n = 25) or XDM (n = 25) were evaluated after 6‐month. Clinical, patient‐centered, and esthetic parameters were assessed. Pearson's correlation and regression analyses were also performed.ResultsGreater recession reduction (RecRed), percentage of root coverage (%RC), and complete root coverage (CRC) were observed in the CTG group (p ≤ 0.02). Gingival thickness (GT) and keratinized tissue width (KTW) gains were higher in the CTG group. The CTG group showed better esthetic at 6‐month. Logistic analyses reported that GT([OR] = 1.6473) and papilla height (PH) (OR = 8.20) are predictors of CRC. GT*XDM interaction was a negative predictor of CRC (OR = 7.105−5). GT at baseline acted as a predictor of RecRed in both groups (p = 0.03). The XDM graft impacted RecRed and %RC negatively. Baseline PH was a predictor of %RC for both grafts.ConclusionCTG was superior for treating RT1/GR, providing better root coverage outcomes and tissue gains over time. GT, PH, and graft type were classified as predictors of CRC.Clinical significanceCTG presented better outcomes to treat single RT1 gingival recession when compared to the XDM.
Microtexture analysis of copper‐doped iron oxide thin films prepared by air pneumatic spray
Authors: Ghribi Faouzi , Ţălu Ştefan , Chouikh Fethi , Bouznit Yazid , Boudour Samah , Méndez‐Albores Alia , Cordova Gabriel Trejo 
Published: June 2022

AbstractThe stereometric and fractal concepts are crucial tools to analyse, to verify, to report 3‐D microtexture of thin film surfaces on the nanometre scale and thereby to generate useful topographic characteristics for better understanding and steering them toward further improvements and rational use in modern applications. At first, the present work aimed to prepare hematite α‐Fe2O3 thin films with (0, 2, 4, 6 and 8 wt%) of Cu doping by using the air pneumatic spray method. Subsequently, the obtained pure α‐Fe2O3 and Cu‐doped α‐Fe2O3 thin films were characterised by XRD device, which determines their polycrystalline nature with the rhombohedral hematite structure. Analysis by UV‐VIS absorption showed that the transmittance of the thin films is extinct in the wavelength from approximately 500 to 800 nm, revealing that the films have good optical absorbance in the visible region. The obtained bandgap values varied between 2.23 and 2.21 eV. At second stage, the stereometric and fractal analysis are applied on 3‐D image data of pure α‐ Fe2O3 and Cu‐doped α‐Fe2O3 thin films, which in prior generated using AFM device. Accordingly, the obtained statistical parameters such as surface roughness, density distribution of peaks, depths etc. were used to understand the influence of Cu doping on the 3D microtexture of pure α‐ Fe2O3 and Cu‐doped α‐ Fe2O3 thin film surfaces.
A critical review of relationship quality measures: Is a fresh start needed? An agenda to move forward
Authors: Gottfredson Ryan K. , Wright Sarah L. , Heaphy Emily D. 
Published: June 2022

AbstractSocial relationships in and around work are a fundamental building block of organizational life. According to a number of relationship theories, the quality of those relationships is a critical aspect of their influence on a variety of employee and organizational outcomes. Relationship quality is, therefore, one of the most commonly studied constructs in management research. Our paper critically reviews the landscape of relationship quality measures used in management studies, identifying critical issues with how the construct has been conceptualized and measured. To help advance research on the subject and improve measurement, we identify and evaluate 21 relationship quality measures that have been developed for, or used in, management research. Overall, we find that the instruments used to measure relationship quality in management research have significant limitations associated with their conceptualizations and operationalizations. Many of these limitations stem from the fact that most of the measures used to assess the construct were not originally designed to measure relationship quality, which impedes research clarity and implications. We offer a future research agenda and several recommendations for the advancement of management research on relationship quality.