bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of omalizumab treatment on gut microbiome in adolescent patients with chronic spontaneous urticaria
Authors: Wang Mei , Zhao Leran , Zeng Sanwu , Qin Yongzhang , Wang Jiangyi , Jin Jingji , Weng Lishou , Wang Dong , You Cong 
Published: April 2022

Abstract
Background: Omalizumab is a humanized anti-immunoglobulin (Ig)E monoclonal antibody that is effective for some patients with chronic spontaneous urticaria (CSU) who do not respond to antihistamines. However, the mechanism by which omalizumab improves urticaria remains obscure. Gut microbiome plays a role in the pathogenesis of allergies. Here, we aimed to investigate differences in gut microbiome of adolescent CSU patients before and after omalizumab treatment, which has not been reported until date. Methods: Ten adolescent patients with CSU were given 300 mg omalizumab subcutaneously in three treatment sessions at 4-week intervals. The personal and clinical factors of patients before and after treatment were collected. Urticaria Activity Score (UAS7) was applied to evaluate the efficacy of omalizumab treatment every 4 weeks during the follow-up period. Fecal samples were collected before treatment and at 12 weeks after the first treatment. Total DNA of the gut microbiota in all fecal samples were extracted. The 16S rRNA gene-targeted sequencing technology was used for the analysis of the diversity and distribution of gut microbiome, followed by bioinformatics analysis. Results: UAS7 scores decreased significantly after each omalizumab treatment sessions compared with the baseline (all P < 0.0001). The dominant bacteria in fecal samples before and after treatment were Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria at the phylum level. Alpha diversity analysis showed no significant difference before and after omalizumab treatment (P > 0.05) whereas beta diversity analysis revealed significant difference in the bacterial abundance before and after omalizumab treatment (P < 0.01) in adolescent patients with CSU. The relative abundance of Alphaproteobacteria and Betaproteobacteria at the class level and Burkholderia, Rhodococcus, and Sphingomonas at the genus level decreased significantly after omalizumab treatment (linear discriminant analysis > 4, P < 0.05). The functional prediction results showed that the differences noted before and after treatment were mainly in the dioxin and xylene degradation pathways, which were more abundant in adolescent patients with CSU before omalizumab treatment.Conclusions: Omalizumab is effective in treating CSU and can reduce the abundance of Alphaproteobacteria and Betaproteobacteria, which may help improve the treatment outcomes of CSU in adolescent patients.
Epigenomics of conventional type-I dendritic cells depicted preferential control of TLR9 versus TLR3 response by NCoR1 through differential IRF3 activation
Authors: Mishra Gyan Prakash , Jha Atimukta , Ahad Abdul , Sen Kaushik , Sen Aishwarya , Podder Sreeparna , Prusty Subhasish , Biswas Viplov K , Gupta Bhawna , Raghav Sunil Kumar 
Published: April 2022

Abstract
Tight control of gene regulation in dendritic cells (DCs) is important to mount pathogen specific immune responses. Apart from transcription factor binding, dynamic regulation of enhancer activity through global transcriptional repressors like Nuclear Receptor Co-repressor 1 (NCoR1) plays a major role in fine-tuning of DC responses. However, how NCoR1 regulates enhancer activity and gene expression in individual or multiple Toll-like receptor (TLR) activation in DCs is largely unknown. In this study, we did a comprehensive epigenomic analysis of murine conventional type-I DCs (cDC1) across different TLR ligation conditions. We profiled gene expression changes along with H3K27ac active enhancers and NCoR1 binding in the TLR9, TLR3 and combined TLR9 + TLR3 activated cDC1. We observed spatio-temporal activity of TLR9 and TLR3 specific enhancers regulating signal specific target genes. Interestingly, we found that NCoR1 differentially controls the TLR9 and TLR3 specific responses. NCoR1 depletion specifically enhanced TLR9 responses as evident from increased enhancer activity as well as TLR9 specific gene expression, whereas TLR3 mediated antiviral response genes were negatively regulated. We validated that NCoR1 KD cDC1 showed significantly decreased TLR3 specific antiviral responses through decreased IRF3 activation. In addition, decreased IRF3 binding was observed at selected ISGs leading to their decreased expression upon NCoR1 depletion. Consequently, the NCoR1 depleted cDC1 showed reduced Sendai virus (SeV) clearance upon TLR3 activation. NCoR1 directly controls the majority of these TLR specific enhancer activity and the gene expression. Overall, for the first time we revealed NCoR1 mediates preferential transcriptional control towards TLR9 as compared to TLR3 in cDC1.
Analysis of the risk of suicide and accidental death in patients with pancreatic cancer in the past 40 years
Authors: Tian Ye , Hou Jingwen , Wang Zonghui , Zheng Rui , Li Ruojin , Liu Yan , Wang Fan 
Published: April 2022

Abstract
Purpose: Data were extracted from the Surveillance, Epidemiology, and End Results (SEER) 18-registry Custom database (1975-2016) to determine the latest risk and risk factors of suicide/accidental death in pancreatic cancer patients. Methods: SEER*Stat was used to get the Standardized Mortality Ratio (SMR) for suicide/accidental death in pancreatic cancer patients. The Adjusted Risk Ratio (ARR) and risk factors for suicide/accidental death in pancreatic cancer patients were achieved by the multivariate Poisson regression model and multivariate Cox regression model.Results: Pancreatic cancer patients diagnosed within three months have the highest SMR of suicide (8.52, 95% Confidence Interval [CI]: 5.71-12.24) and accidental death (2.75, 95% CI: 1.98-3.72); Among the pancreatic cancer patients hadn’t performed surgery, patients classified as ‘Recommended but not performed’ have a higher ARR of suicide(3.35, 95% CI: 2.22-4.87) and accidental death(2.12, 95% CI: 1.53-2.87) than patients classified as ‘Not recommended’, the same trend also showed in the multivariate Cox regression model: The Hazard Ratio (HR) of ‘Recommended but not performed’ patients for suicide(Vs ‘Performed’, 2.68, 95% CI: 1.36-5.30) and accidental death(Vs ‘Performed’, 2.68, 95% CI: 1.36-5.30) higher than ‘Not recommended’ patients.Conclusion: Pancreatic cancer patients have a significantly higher risk of suicide and accidental death than the general population and other cancer patients, especially within three months of diagnosis. In addition, we found in the pancreatic cancer patients who hadn’t performed surgery, those ‘Recommended but not performed’ have a higher risk of suicide and accidental death than those ‘Not recommended’. Therefore, we suggest Department doctors collaborate with oncology psychologists and maintain regular touch with patients who are ‘Recommended but not performed’ and diagnosed within three months.
Fibroblasts And Mouse's Breast Cancer Cells Can Form Cellular Aggregates in Improved Soft Agar Culture Medium
Authors: Zhang Xiangnan , Liang Shuo , Wang Enze , Tao Ning 
Published: April 2022

Abstract
Purpose
Cancer-associated fibroblasts (CAFs) promote cancer cell growth, invasion and migration. In this research, we aimed to build cellular aggregates of mouse’s breast cancer cells (TS/A) and normal fibroblasts (LX-2) or CAFs (ME-iLX-2) in soft agar culture medium, verifying this co-culture model’s value in screening of anticancer drugs and preliminarily demonstrating the effect of CD44 in aggregates formation.
Methods
We improved soft agar culture medium to co-culture CAFs (NFs) and TS/A to build the cellular aggregates model, and compared the amount and area of different co-culture groups. To verify its value in screening of anticancer drugs, eugenol which has been demonstrated its anticancer effect was added into this model. The transcription of human CD44 was analyzed through Real-time quantitative PCR (RT-qPCR).
Result
TS/A-fibroblast cellular aggregates were formed in improved soft agar culture medium. What’s more, the amount and area of aggregates in TS/A-ME-iLX-2 co-culture group were significantly higher than in TS/A-LX-2 co-culture group. In co-culture group with eugenol, the amount and area of TS/A-ME-iLX-2 aggregates were lower than control group. The result of RT-qPCR showed that the transcription volume of human CD44 in TS/A-ME-iLX-2 aggregates was higher than in TS/A-LX-2 aggregates.
Conclusion
Co-culturing cellular aggregates of fibroblasts and TS/A was successfully formed in improved soft agar culture medium, and the amount and area of aggregates further confirmed CAFs’ promotion effect to cancer cells. Eugenol test showed its value in screening of anticancer drugs. RT-qPCR result demonstrated the important effect of CD44 in aggregates formation.
A Quality Assessment of Information Provided on Websites Selling Cannabis to Consumers in Canada
Authors: Ng Jeremy Y , Tahir Umair , Lum Nicholas 
Published: April 2022

Abstract
Background: Cannabis is used by millions of people for both medical and recreational purposes, and this use is even greater in jurisdictions where it is legalized, such as Canada. Online cannabis vendors have gained popularity for purchasing cannabis due to the easy access and convenience to consumers. The objective of this study was to evaluate the quality of health information provided by websites of cannabis vendors selling products to Canadian consumers, and to further identify trends in the information provided. Methods: Six different searches were conducted on Google.ca, and the first 40 webpages of each search were screened for eligibility. A total of 33 unique websites of cannabis vendors selling products to Canadian consumers were identified and included. The DISCERN instrument, which consists of 16 questions divided into three sections, was used to evaluate the quality of cannabis-related health information on these websites. Results: Across the 33 websites, the average of the summed DISCERN scores was 36.83 (SD = 9.73) out of 75, and the mean score for overall quality of the publication (DISCERN question 16) was 2.41 (SD=0.71) out of 5. Many of these websites failed to discuss uncertainties in research evidence on cannabis, the impact of cannabis use on quality of life, alternatives to cannabis use, risks associated with cannabis use, and lacked references to support claims on effects and benefits of use.Conclusion: Our findings indicate that the quality of cannabis-related health information provided by online vendors is poor. HCPs should be aware that patients may use these websites as primary sources of information, and appropriately caution patients while directing them to high-quality sources. Future research should serve to replicate this study in other jurisdictions and assess the accuracy of information provided by online cannabis vendors, as this was outside the scope of the DISCERN instrument.
Insolvency Resolution and Doing Business Reforms in BRICS Nations
Authors: Chawla Neelam , Kumar Basanta , Patel Gokulananda 
Published: April 2022

Abstract
This paper analyzes the factors responsible for the efficacy of resolving insolvency issues influencing the ease of doing business (EoDB) ranking in the BRICS (Brazil-Russia-India-China-South Africa) nations' context, with particular reference to India. Based on published data from the World Bank's EoDB reports, the paper analyzes the impact of EoDB parameters, particularly resolving insolvency issues, on its ranking. Besides, the study examines the legal framework of insolvency resolution that boosts stakeholders' confidence and improves the business environment. The research also investigates the legal framework of insolvency resolution vis-a-vis judicial pronouncement to understand government strategies, stakeholders' trust and the business environment aiding EoDB ranking. Descriptive statistics and analytical hierarchy process (AHP) were applied to analyze and interpret the findings and results. The key findings show that insolvency resolution has been one of the priorities of the governments in Brazil, Russia, and China almost during the last decade; India's importance came after the Insolvency and Bankruptcy Code (IBC), 2016 enactment. Resolving insolvency is the prime factor responsible for India's rank improvement, whereas it is not a priority for South Africa. The AHP in the Indian context proves that the government's importance to insolvency resolution moves in lockstep with expert opinions. The findings add value to the body of knowledge on insolvency resolution, contributing to the EoDB ranking vis-a-vis the business environment, and provide a broader implication for further research.
WITHDRAWN: Deep Learning Based CSI Feedback for Massive MIMO Aided IoT Networks with Edge Computing
Authors: Wu Di , Liu Zhusong , Sun Li , Li Chao , Zhou Wei , Li Yun , Wu Rui 
Published: April 2022

Abstract

Let us suppose we are given a complete, complete modulus H''. Recently, there has been much interest in the derivation of hulls. We show that Dⲉ,a is p-Russell and unique. Thus in this setting, the ability to compute fields is essential. It would be interesting to apply the techniques of [1] to embedded systems. It has long been known that ||ⲱ|| ≥ ℵ0 [1]. Moreover, is it possible to describe Einstein, globally linear, uncountable isometries? It has long been known that Ḡ is not greater than e'' [1]. On the other hand, the goal of the present paper is to construct co-compactly affine, finitely co-dependent subrings. In [1], the authors studied almost everywhere hyper-hyperbolic primes. Hence it is well known that there exists a linearly covariant functional. The groundbreaking work of J. Sato on Hermite random variables was a major advance.
Relativistic effect inside matter. Obtaining a relativistic dispersion formula.
Authors: Chernov Andrey 
Published: April 2022

Abstract
The main prerequisite for this study was the external similarity of such physical processes as the refraction of light in a transparent substance and the deflection of light by the gravitational field of massive cosmic bodies. Based on the external similarity, the author put forward a hypothesis about the internal similarity of these two physical processes. This made it possible to integrate Einstein's well-known relativistic formula into the process of light propagation in electron clouds (fields) of a transparent substance. As a result of the transformations carried out, a new physical dispersion formula with a wide spectrum of action was obtained from the Einstein formula. According to the new dispersion formula, 26 refractive indices of light were calculated in 5 transparent substances in 3 states of aggregation. Comparison of the obtained indicators with laboratory indicators showed the high accuracy of the new physical formula, which amounted to ±10 -7−10 -5 in the calculated sections of the wave ranges over 100 nm. The successful application of the relativistic formula to the process of light refraction in transparent substances indicates that relativistic effects are present at the atomic-molecular level.
Relevansi Nilai Dasar Bela Negara Dengan Pembayaran Zakat dan Implikasinya Terhadap Ketahanan Pribadi
Authors: ROHIM ADE NUR 
Published: April 2022

ABSTRACT This article aimed to examined the relevance of the basic values of state defense with the activity of paying zakat, so that the approach taken was ideological, which had implications for the realization of personal resilience. The research used a qualitative method through descriptive content and literature study. The data analyzing process used were collecting data, reducing data, presenting data, and drawing conclusion.The results of the study showed that zakat beside that it was an obligation and pillar of Islam for every Muslim, it was also an activity that implemented the basic values of state defense and internalizing them into the attitudes and behavior of those who payed zakat. The values that were implemented include loving the country, being aware of the nation and state, believing in Pancasila as the state ideology, being willing to sacrificed for the nation and state, and having the initial ability to defended the state. Thus, the value relevance and wisdom contained in zakat on the basic value of defending the country had a positive impact on the formation of the personal resilience of those who payed zakat. The behavior of paying zakat directly fostered a patriotic spirit and loved for the motherland in him and strengthened his belief in Pancasila, which was the nation's ideology and contributed to increased self-sacrifice for the benefit of religion and the nation.ABSTRAK Artikel ini bertujuan untuk mengkaji relevansi nilai-nilai dasar bela negara dengan aktivitas membayar zakat, sehingga pendekatan yang dilakukan bersifat ideologis yang berimplikasi pada perwujudan ketahanan pribadi.Metode penelitian yang digunakan adalah metode kualitatif melalui pendekatan deskriptif dan studi pustaka. Proses analisis data dilakukan dengan pengumpulan data, reduksi data, penyajian data, dan penarikan kesimpulan.Hasil kajian menunjukkan bahwa zakat selain menjadi kewajiban dan rukun Islam bagi setiap muslim, ia juga merupakan aktivitas yang mengimplementasikan nilai-nilai dasar bela negara dan menginternalisasikannya ke dalam sikap dan perilaku orang yang membayar zakat. Nilai-nilai yang diimplementasikan antara lain cinta tanah air, sadar berbangsa dan bernegara, yakin pada Pancasila sebagai ideologi negara, rela berkorban untuk bangsa dan negara, serta memiliki kemampuan awal bela negara. Dengan demikian, relevansi nilai dan hikmah yang terkandung di dalam zakat terhadap nilai dasar bela negara memberikan dampak positif terhadap pembentukan ketahanan pribadi orang yang membayarkan zakat. Perilaku membayar zakat tersebut secara langsung menumbuhkan jiwa patriot dan cinta tanah air dalam dirinya, serta meneguhkan keyakinannya terhadap Pancasila yang merupakan ideologi bangsa, dan turut meningkatkan sikap rela berkorban untuk kepentingan agama dan bangsa.
Penerapan Strategi Pemanduan Terpadu Sebagai Upaya Mewujudkan Ketahanan dan Keamanan Di Selat Malaka
Authors: Hutabarat TSNB , Maarif Syamsul , Zulbainarni Nimmi , Yusgiantoro Purnomo 
Published: April 2022

ABSTRACT Malacca Strait was the busiest strait the world, where most of distribution lines of sea transportation pass through. However, there were some issues in this area, including security and safety issues, which could interfere the business operation and threaten national resilience. On the other hand, the high cost of the efforts to maintained the security and safety in the area burdens the government. Departing from this background, this research aimed to developed a strategy in handling sea transportation system in order to achieved the equilibrium between economy efficiency and the effectiveness of the government in maintaining security and safety of the shipping traffic in Malacca Strait. This study was a problem solving-based research using Soft System Methodology and Analytical Hierarchy Process. As the results, The Integrated Piloting Strategy had been developed as a recommendation to be implemented. With this strategy the ships that passed through the Malacca Strait were advised to adopted Integrated Piloting, in which the Pilots together with the security guards, would be on board and helped the ship to passed through Malacca Strait safely and securely. By that, the safety and security were guaranteed and the business objectives were achievedABSTRAK Moda transportasi laut merupakan sarana utama distribusi logistik di dunia. Sebagian besar alur distribusi ini melewati wilayah perairan Selat Malaka, menjadikan Selat Malaka sebagai selat tersibuk di dunia, yang mana 75 persen perairannya merupakan wilayah Negara Kesatuan Republik Indonesia (NKRI). Namun demikian terdapat beberapa permasalahan di wilayah Selat Malaka tersebut, di antaranya permasalahan keamanan serta keselamatan yang berpotensi mengganggu kelancaran operasional bagi para pelaku bisnis serta mengancam ketahanan nasional. Di sisi lain upaya menjaga keamanan serta keselamatan di wilayah tersebut membebani Pemerintah dengan biaya yang sangat besar. Berdasarkan latar belakang tersebut penelitian ini bertujuan untuk merumuskan strategi di dalam menangani sistem transportasi laut agar terjadi kesetimbangan antara efisiensi ekonomi bagi pelaku bisnis, serta efektivitas instansi pemerintah dalam menjaga keamanan dan keselamatan pelayaran di Selat Malaka. Studi ini merupakan penelitian berbasis problem solving menggunakan metode Soft System Methodology (SSM) dan Analytical Hierarchy Process (AHP). Sebagai hasil dari penelitian ini disusun rekomendasi berupa Strategi Pemanduan Terpadu di mana kapal-kapal yang melintasi Selat Malaka disarankan untuk menggunakan Pemanduan Terpadu yaitu petugas Pandu yang terintegrasi dengan keberadaan petugas keamanan yang turut on board di kapal selama melintas di Selat Malaka, dengan segala tantangannya, sehingga keamanan dan keselamatan terjamin, keuntungan bisnis tercapai serta terwujud efisiensi ekonomi bagi semua pihak yang terlibat..
Strategi Komunikasi Dan Pemanfaatan Teknologi Informasi Dan Komunikasi Dalam Pengembangan Ketahanan Desa Wisata Pada Masa Pandemi Covid-19 Di Cirebon
Authors: Subejo Subejo , Chamidah Nurul , Nirmalasari Nirmalasari , Suyoto Suyoto , Hariadi Sunarru Samsi , Muhamad Muhamad , Selvi Apredeah Monica , Siddiq Dedi Muhammad , Imawan Khaerudin , Isamayana Isamayana 
Published: April 2022

The governance of tourism villages, synergy and coordination among tourism village stakeholders that are efficient and effective and strengthening the capacity and support capacity of stakeholders requires a communication strategy for developing tourism villages that is right in line with advances in information technology which have great potency to facilitate various tourism village development activities. In addition, the latest global developments with the Covid-19 pandemic appear to have a significant impact on the development of tourist villages. The study was conducted in three tourist villages in Jamblang District, Cirebon Regency, which have initiated the tourism village since 2016. Several research techniques used were FGD, in-depth interviews and direct interviews with 95 respondents. The study is able to map stakeholders based on status (internal and external) and stakeholders based on the level of interest and contribution (primary, secondary and tertiary). The main relationships between stakeholders include: socialization, training, extension, reports, coordination, promotion and assistance. Formally, there is no communication strategy document available, but related parties have practiced communication according to their interests. New media and several applications or platforms have begun to be used by the community in supporting the development of tourism villages. The development of tourist villages was significantly affected by the Covid-19 pandemic and the existing strategies are not yet adaptive enough to the problems that arise. Several prospective tourism village development strategies include improving infrastructure aspects, human resource capacity, diversifying tourist attractions and strengthening promotions.