bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel CREBBP mutation and its phenotype in a case of Rubinstein-Taybi syndrome
Authors: Wang Qian , Wang Cong , Wei Wen Bin , Rong Wei Ning , Shi Xiang Yu 
Published: May 2022

Abstract
This study was to report a novel CREBBP mutation and phenotype in a child with Rubinstein-Taybi syndrome. We described the patient’s clinical manifestations in detail, and found that in addition to the typical systemic manifestations of the syndrome, the outstanding manifestation of the child was severe intellectual deficiency and prominent ocular abnormalities. Whole-exome sequencing and sanger sequencing were performed on the patient and his parents, a large intragenic deletion, covering the exon 1 region and part of the intron 1 region of the TRAP1 gene, and the entire region from intron 27 to exon 30 of the CREBBP gene (chr16:3745393-3783894) was identified on the patient. This mutation affected the CREBBP histone acetyltransferase (HAT) domain. This findings in our patient add to the spectrum of genetic variants described in Rubinstein-Taybi syndrome and present a RSTS patient with various ocular anomalies including early onset glaucoma.
The potential of genome-wide prediction to support parental selection, evaluated with data from a commercial barley breeding program
Authors: Rembe Maximilian , Zhao Yusheng , Wendler Neele , Oldach Klaus , Korzun Viktor , Reif Jochen 
Published: May 2022

Abstract
Parental selection marks the beginning and contributes significantly to the success of every breeding endeavor. The value of a cross is reflected in the potential of its progeny population. Breeders invest money, time, and space into the evaluation of progeny to select the best performing genotypes as candidates for variety development. Several proposals to support parental selection by genomics have been made, mostly based on theoretical assumptions or simulation studies. While most of them claim to provide the solution to the problem of parental selection, only few consider practical hurdles connected to their implementation or are solely based on simulations. In this study, we tested the potential of genomic prediction for predicting progeny mean, variance and usefulness criterion in an applied breeding population for winter barley. For three traits with genetic architectures at varying levels of complexity, ear emergence, plant height, and grain yield, progeny mean, variance, and usefulness criterion were predicted and validated in scenarios resembling situations in which the described tools shall be used in plant breeding. We showed that the identification of superior crosses remains a challenging task and suppose that the success for the prediction of the usefulness criterion depends highly on the complexity of the underlying trait.
Evaluating the prognostic value of CD56 in pediatric acute myeloid leukemia
Authors: Liang Tianqi , Peng Zhiyong , Li Chunfu , Huang Junbin , Wang Huabin , Bu Chaoke , Li Jian , Zheng Yongzhi , Feng Xiaoqin , Li Huiping , Chen Chun 
Published: May 2022

Abstract
Background：Many cytogenetic changes and gene mutations are associated with acute myeloid leukemia (AML) survival outcomes. CD56 is related to poor prognosis when expressed in adult AML patients. However, the prognostic value of CD56 in children with AML has rarely been reported. In this research, we aimed to evaluate the prognostic value of CD56 in childhood AML.Methods：The present retrospective study included 145 newly diagnosed pediatric patients with de novo AML (excluding AML-M3) in two hospitals between January 2015 and April 2021.Results：The total median (range) age was 75 (8-176) months, and the median follow-up time was 35 months. No significant difference in the 3-year overall survival rate was noted between the CD56-positive and CD56-negative groups (67.0% vs. 79.3%, P=0.157) who received chemotherapy. However, among high-risk patients, the CD56-positive group had a worse overall survival rate and event-free survival rate (P<0.05). Furthermore, among high-risk patients, the CD56-positive group had higher relapse and mortality rates than the CD56-negative group (P<0.05). Conclusions：CD56 represents a potential factor of poor prognosis in specific groups of children with AML and should be considered in the risk stratification of the disease. Given the independent prognostic value of CD56 expression, we should consider integrating this marker with some immunophenotypic or cytogenetic abnormalities for comprehensive analysis.
Photocatalytic degradation of methyl orange behavior comparison of titanium species: alkaline biochar and acidic biochar composites
Authors: Huang Hsin-Liang , Huang Zi-Hao , Zhou Wan-Jia 
Published: May 2022

Abstract
The biochar was produced from wood waste via pyrolysis. The biochar with pH 9.7 was comprised of titanium species named Ti-WB9. After acidification, the acidic biochar with pH 3.66 was also comprised of titanium species named Ti-WB3. Using XRD, the particle sizes of anatase TiO2 were 7 and 8 nm in Ti-WB9 and Ti-WB3, respectively; these were then synthesized via the solvothermal method at low temperature. The degradation efficiencies of methyl orange were 93.5 and 95.1% in 120 min with Ti-WB9 and Ti-WB3, respectively. During degradation, the by-products were barely observed in the solution when Ti-WB3 was used as a photocatalyst under UV light. However, the by-products exited in another solution that used Ti-WB9 as a photocatalyst for degradation according to the results of UV-Vis and FTIR. Via XPS, the formation of C-Ti in Ti-WB3 played an important role in the enhanced and complete degradation of methyl orange without by-products.
Design, synthesis, and anti-bacterial activities of piperazine based phthalimide derivatives against superbug-MRSA
Authors: S Nagendra Prasad H , Ananda A. P. , Amogh P.M. , Gaonkar Navyatha Prashanth , Jayanth H. S. , Sumathi S. , Krishnamurthy N. B. , Mallesha L. , Mallu P. 
Published: May 2022

Abstract
A series of piperazine-based phthalimide derivatives 5 (a-l) were synthesized and extensively characterized using a variety of spectrum methods, including LC-MS, 1H-NMR, 13C-NMR, and FT-IR. All the derivatives were examined for their physicochemical, pharmacokinetic, bio-activity score, and PASS analysis. The 5e piperazine-based phthalimide derivative demonstrated outstanding antibacterial activity against MRSA in the in vitro antibacterial studies. In comparison to streptomycin and bacitracin (10 µg/mL), the minimum inhibitory concentration of 5e against MRSA was discovered to be 45 ± 0.15 g/mLThe anti-MRSA activity was validated with membrane damage studies by using SEM, and in silico docking studies were against 3VMT and 6FTB proteins of MRSA. In the toxicity study, 5e derivatives were evaluated against L6 cell lines. The results of the studies show the synthesized 5e derivative can be used for the development of anti-MRSA drugs.
Effects of Coix seed extract, Lactobacillus paracasei K56, and their combination on the glycolipid metabolism of obese mice
Authors: Zhang Wei , Jia Xiuzhen , Xu Yuhan , Xie Qiaoling , Zhu Meizhen , Zhao Zifu , Hao Jingyu , Li Haoqiu , Du Jinrui , Liu Yan , Liu Wei-Hsien , Ma Xia , Hong Weilian , Tian Fenghao , Li Hongwei 
Published: May 2022

Abstract
Coix seed extract (CSE) and probiotics have been reported to regulate glycolipid metabolism through different modes of action. We tested the effects of CSE, Lactobacillus paracasei K56, and their combination to determine whether they have synergistic effects on glycolipid metabolism of obese mice. We fed male C57BL/6J mice with high-fat diet for 8 weeks to establish an obesity model. The obesity mice were selected and divided into five groups: the model control group and 4 intervention groups. After 10 weeks of continuous gavage intervention, the mice in the intervention groups exhibited lower body weight, and epididymal and perirenal fat content; decreased fasting blood glucose, total cholesterol, triglycerides, and VLDL; and increased HLDL, respiratory exchange ratio, energy expenditure, and amount of exercise performed. K56 + CSE combined intervention groups were more effective in lowering blood glucose, IL-1β, and TNF-α levels than the CSE and K56 alone interventions. The content of FAS and SREBP-1c protein in liver tissue was lower. The combination has synergistic effects on weight control, fat reduction and blood glucose regulation by improving the chronic inflammatory state and reducing the content of lipid synthesis-related enzymes of obese mice, which can hinder chronic disease progression.
Gamma ray irradiation behavior and damage mechanism of the “Pandan” type Polarization-Maintaining Optical Fibers
Authors: Zhang Hongchen , Xue Hhuijie 
Published: May 2022

Abstract
Gamma ray irradiation experiments were carried out on “Panda” type polarization-maintaining optical fiber made in China, and the relationship between irradiation induced loss and dose and dose rate at 1310 nm of the polarization-maintaining optical fiber was studied. By means of XPS, EPR, FTIR and loss spectrum analysis, the damage mechanism of gamma ray irradiated “Panda” type polarization-maintaining optical fiber is discussed. The results show that for the reason of gamma ray irradiation, the active particles .H are produced in the coating material, and the impurity defects of Si-OH are formed with the products of SiO2 irradiated by gamma ray. The concentration of impurity and defect in Si-OH is the key factor that affects the attenuation coefficient of polarization-maintaining optical fiber with “Panda” type at 1310nm.
Canola oil-based aqueous polyurethane dispersion finishes for cellulosic modification of the textile fabric
Authors: . Sidra , Tabasum Shazia , Zia Khalid Mahmood , Parveen Bushra , Rehman Saima 
Published: May 2022

Abstract
Since government regulations have forced manufacturers to minimise volatile organic compounds and consumer trends drive the demand for environmentally friendly products, polyurethane formulations have shifted away from solvent-based polyurethane formulations toward aqueous polyurethane dispersions. Because of the current need in this work, a renewable canola oil-based polyol derived from canola oil was synthesised by the epoxidation and oxirane ring-opening procedure and polymerized with different diisocyanates, dimethylol propionic acid, and 1,4-butanediol, used to produce waterborne PU dispersion. The structural characterization of the prepared polyol and dispersions was carried out by FTIR spectroscopy. The synthesised dispersions were applied to the poly/cotton fabrics by the pad-dry-cure process and their textile performances, such as pilling resistance and color fastness properties, were investigated. The antibacterial activity of the finished applied fabrics was also evaluated against gram-positive and gram-negative bacteria. All the samples have shown remarkable enhancement in both anti-pilling (1/2 to 3/4 in dyed) and color fastness properties (such as rubbing in dyed, dry 3/4 to 4/5, and wet 3 to 4) as the solution concentration of the dispersions increases. The results reveal that canola oil-based PU dispersions applied as a finish to the poly/cotton fabrics enhanced their textile as well as antibacterial performance.
U2AF2 promote the proliferation and migration of esophageal adenocarcinoma cells by sustaining the stability of SNORA21
Authors: Gao Lijuan , Chen Yongshun , Li Bin 
Published: May 2022

Abstract
Purpose
This study is aimed to investigate the role and mechanism of U2AF2 in esophageal adenocarcinoma cell line.
Methods
The expression level of U2AF2 and SNORA21 was analysed by TCGA database. The expression level of U2AF2 and SNORA21, Bax, Bcl-2 and E-cadherin were determined by Western Blot and RT-qPCR assay. The cell viability and proliferation were detected by CCK8 assay and BrdU incorporation assay. The apoptosis was examined by caspase3 immunocytofluorescense and flow cytometry assay. The interaction between U2AF2 and SNORA21 was predicted by Starbase 3.0 and confirmed by RIP assay. The expression pre-mRNA of U2AF2 and SNORA21 was detected by Nuclear Run On assay.
Results
The expression of U2AF2 and SNORA21 was found to be upregulated and served as an unfavourable factor which negatively related with overall survival of esophageal adenocarcinoma patients. Silencing the expression of U2AF2 or SNORA21 could suppress the proliferation and migration of OE19 and OE33 cells and stimulate its apoptosis. Moreover, we identified that the U2AF2 could bind and interact with SNORA21 to sustain its stability to regulate the viability of esophageal adenocarcinoma cells. Moreover, U2AF2-OE could accelerate the proliferation and migration ability of normal esophageal cell lines HET-1A by SNORA21.
Conclusion
Our study provides several lines of evidence supporting the intriguing concept that U2AF2 could promote the proliferation of OE19 and OE33 cells by directly targeting SNORA21, which might serve as a novel candidate biomarker and a new target for the treatment of esophageal adenocarcinoma.
Hypverventilation Strain CMR Imaging in Patients With Acute Chest Pain
Authors: Siry Deborah , Riffel Johannes H. , Salatzki Janek , Andre Florian , Ochs Marco , Weberling Lukas D. , Giannitsis Evangelos , Katus Hugo A. , Friedrich Matthias G. 
Published: May 2022

Abstract
Background
In patients with suspected acute coronary syndrome (ACS) high-sensitivity cardiac tropnonin T (hscTnT) is used for rapid patient triage. Fast-strain encoded (fSENC) CMR imaging with breathing maneuvers may accelerate diagnostic work-up and identify patients suffering from ACS.
Methods
Patients presenting with ACS (hscTnT level 5-52ng/L) were prospectively enrolled. fSENC imaging was performed within the 1-hour timeframe (0h/1h algorithm) prior to 2nd hscTnT lab results. Images were acquired at rest as well as after 1-minute of hyperventilation followed by a short breath-hold.
Results
In 108 patients (58 male; mean age: 57±17y) the mean study time was 17 ± 3 minutes. An abnormal strain response after the breathing maneuver correctly identified all 17 patients with a hscTnT dynamic (0h/1h algorithm) and explanatory significant coronary lesions, while in 86 patients without serologic or angiographic evidence for severe coronary artery disease the strain response was normal (sensitivity 100%, specificity 94.5%; 5 false positive results). The number of dysfunctional segments (strain>-10) proved to be a quantifiable marker for identifying patients with ACS.
Conclusions
In patients with suspected ACS and inconclusive initial hscTnT, fSENC imaging with a breathing maneuver may safely and rapidly identify patients with ACS, without the use of stress or contrast agents/ medication.
Development and Validation of a Kidney-Transplant Specific Measure of Treatment Burden
Authors: Lorenz Elizabeth C. , Petterson Tanya M. , Zaniletti Isabella , Lackore Kandace A. , Johnson Bradley K. , Mai Martin L. , Nair Sumi S. , Bentall Andrew , Yost Kathleen J. , Eton David T. 
Published: May 2022

Abstract
Background
Treatment burden refers to the work involved in managing one’s health and its impact on well-being and has been associated with nonadherence in patients with chronic illnesses. No kidney transplant (KT)-specific measure of treatment burden exists. The aim of this study was to develop a KT-specific supplement to the Patient Experience with Treatment and Self-Management (PETS), a general measure of treatment burden.
Methods
After drafting and pretesting KT-specific survey items, we conducted a cross-sectional survey study involving KT recipients from Mayo Clinic in Minnesota, Arizona, and Florida. Exploratory factor analysis (EFA) was used to identify domains for scaling the KT-specific supplement. Construct and known-groups validity were determined.
Results
Survey respondents (n = 167) had a mean age of 61 years (range 22–86) and received a KT on average 4.0 years ago. Three KT-specific scales were identified (transplant function, self-management, adverse effects). Higher scores on the KT-specific scales were correlated with higher PETS treatment burden, worse physical and mental health, and lower self-efficacy (p < 0.0001). Patients taking more medications reported higher transplant self-management burden.
Conclusions
We developed a KT-specific supplement to the PETS general measure of treatment burden. Scores may help providers identify recipients at risk for nonadherence.
Time will tell: Larger and less abundant fruit-feeding butterflies (Lepidoptera: Nymphalidae) are synchronized with food availability in the Brazilian Cerrado
Authors: Freire Geraldo Brito , Salscido Danielle , Oliveira Hernani F. M. , Ribeiro Danilo B. , Provete Diogo B. , Silva Thayane , Dias Joao Paulo , Rodrigues Hanna P. , Santos Jessie P. , Diniz Ivone R. 
Published: May 2022

Abstract
Introduction: Diet and temporal specialists, especially those in tropical seasonal environments, require synchronous phenology with hosts. Species with life-history-traits that covary with temporal specialization may be particularly vulnerable to climate-driven changes in phenology. In the Cerrado, larger fruit-feeding butterflies species tend to be less abundant and more seasonal than their smaller-bodied relatives. It is important to consider how ecological process and phylogenetic history are associated with these attributes. Aim/Methods: Here we evaluated the effects of climate and body size on the seasonality of abundance of fruit-feeding butterflies as well as their association with natural history traits including diet breadth and synchrony with fruits.Results: The distribution of abundances of these butterflies species was not uniform throughout the year, was associated with body size and shaped by habitat type and climate. Overall, hot and wet months were associated with higher abundance and species richness of these nymphalids in the Cerrado. Lineages of large-bodied species were less abundant, more seasonal and more generalized in larval diet breadth. In addition, these large-bodied clades were more tightly synchronized with fruit phenology.Discussion: Our findings support the hypothesis that large and less abundant species to occur in a narrow temporal window and synchronized with fruit phenology. Implications for insect conservation: Particular attention should be given for the conservation of larger-bodied temporal specialist species as they are vulnerable to phenology mismatching as a result of rapid global warming, but they will also face additional pressures with the advancing deforestation in the Cerrado.
A new polysaccharide platform constructs self-adjuvant nanovaccines to enhance immune responses
Authors: Chen Sisi , Yang Liu , Ou Xia , Li Jin-Yu , Zi Cheng-Ting , Wang Hao , Hu Jiang-Miao , Liu Ye 
Published: May 2022

Abstract
BackgroundNanovaccines have shown the promising potential in controlling and eradicating the threat of infectious diseases worldwide. There has been a great need in developing a versatile strategy to conveniently construct diverse types of nanovaccines and induce potent immune responses. To that end, it is critical for obtaining a potent self-adjuvant platform to assemble with different types of antigens into nanovaccines. ResultsIn this study, we identified a new natural polysaccharide from the rhizomes of Bletilla striata (PRBS), and used this polysaccharide as a platform to construct diverse types of nanovaccines with potent self-adjuvant property. In the construction process of SARS-CoV-2 nanovaccine, PRBS molecules and RBD protein antigens were assembled into ~300 nm nanoparticles by hydrogen bond. For HIV nanovaccine, hydrophobic effect dominantly drove the co-assembly between PRBS molecules and Env expression plasmid into ~350 nm nanospheres. Importantly, PRBS can potently activate the behaviors and functions of multiple immune cells such as macrophages, B cells and dendritic cells. Depending on PRBS-mediated immune activation, these self-adjuvant nanovaccines can elicit significantly stronger antigen-specific antibody and cellular responses in vivo, in comparison with their corresponding traditional vaccine forms. Moreover, we also revealed the construction models of PRBS-based nanovaccines by analyzing multiple assembly parameters such as bond energy, bond length and interaction sites. ConclusionsPRBS, a newly-identified natural polysaccharide which can co-assemble with different types of antigens and activate multiple critical immune cells, has presented a great potential as a versatile platform to develop potent self-adjuvant nanovaccines.
A personalized multi-interventional approach focusing on customized nutrition, progressive fitness, and lifestyle modification resulted in the reduction of HbA1c, fasting blood sugar and weight in type 2 diabetes: A retrospective study
Authors: Mehra Chhavi , Raymond Annie Mattilda , Prabhu Rekha 
Published: May 2022

Abstract
Background: Type 2 diabetes (T2D) is a chronic, progressive lifestyle disease and the most rapidly growing health challenge of the 21st century. The American Diabetes Association recommends that T2D reversal can be achieved through an organized, and systematic approach focusing on nutrition, fitness, and lifestyle management. Aim: This study aimed to evaluate the effectiveness of a comprehensive and multi-interventional diabetes care program called Sugar.Fit Diabetes Reversal Programme (SDRP) on glycosylated haemoglobin (HbA1c), fasting blood sugar (FBS), and body weight for T2D reversal.Methodology: SDRP is a personalized intervention study that uses technology-enabled medical management, dedicated coach-led diabetes, and nutrition experts. The study involved 150 patients living with type 2 diabetes in the age group of 20 to 80 years and having HbA1c of >6.5%. In SDRP, the participants were assigned personal medical doctors specializing in diabetes, along with health coaches for providing customized nutrition, personalized fitness routines, relevant lifestyle modifications to holistically reverse type 2 diabetes Type 2 diabetes. The HbA1c level, fasting blood glucose, and weight of the participants were measured at baseline and the end of the study (90th day). The effectiveness of SDRP was analyzed by comparing it with a control group that involved 110 individuals with type 2 diabetes managed by conventional pharmacotherapy and regular dietary advice but not participating in the SDRP.Results: All 150 participants adhered to the program for 90 days. The analysis was performed on participants and represented as mean ± standard deviation (mean ± SD). At the end of SDRP, a significant reduction in HbA1c level, FBS, and weight was observed as compared to the control group. The results showed that Hba1c levels dropped from 9.0±1.5% to 7.1±1.3% with a mean change of 1.9±1.5%; FBS levels decreased from 178.3±57.1 mg/dL to 116.1±24.2 mg/dL with a mean loss of 62.2±51.8 mg/dL, and the weight decreased from 76.7±12.7 kg to 73.8±11.8 kg with a mean weight loss of 2.8±1.6 kg. The results also showed that participants between 20 to 35 years showed the highest drop in HbA1c, FBS, and weight.Conclusion: The findings indicate that a comprehensive and multi-interventional diabetes care program involving personalized nutrition, fitness, and lifestyle modification such as SDRP, help in significant and sustained improvements in HbA1c level, glycaemic control, and weight loss in adults with type 2 diabetes.