bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing Seroma Formation at the Deep Inferior Epigastric Perforator, Transverse Musculocutaneous Gracilis, and Superior Gluteal Artery Perforator Flap Donor Sites after Microsurgical Breast Reconstruction
Authors: Merchant Alisha , Speck Nicole E. , Michalak Michal , Schaefer Dirk J. , Farhadi Jian 
Published: August 2022

Abstract
Background Seroma formation is the most common donor site complication following autologous breast reconstruction, along with hematoma. Seroma may lead to patient discomfort and may prolong hospital stay or delay adjuvant treatment. The aim of this study was to compare seroma rates between the deep inferior epigastric perforator (DIEP), transverse musculocutaneous gracilis (TMG), and superior gluteal artery perforator (SGAP) donor sites.
Methods The authors conducted a retrospective single-center cohort study consisting of chart review of all patients who underwent microsurgical breast reconstruction from April 2018 to June 2020. The primary outcome studied was frequency of seroma formation at the different donor sites. The secondary outcome evaluated potential prognostic properties associated with seroma formation. Third, the number of donor site seroma evacuations was compared between the three donor sites.
Results Overall, 242 breast reconstructions were performed in 189 patients. Demographic data were found statistically comparable between the three flap cohorts, except for body mass index (BMI). Frequency of seroma formation was highest at the SGAP donor site (75.0%), followed by the TMG (65.0%), and DIEP (28.6%) donor sites. No association was found between seroma formation and BMI, age at surgery, smoking status, diabetes mellitus, neoadjuvant chemotherapy, or DIEP laterality. The mean number of seroma evacuations was significantly higher in the SGAP and the TMG group compared with the DIEP group.
Conclusion This study provides a single center's experience regarding seroma formation at the donor site after microsurgical breast reconstruction. The observed rate of donor site seroma formation was comparably high, especially in the TMG and SGAP group, necessitating an adaption of the surgical protocol.
Facial Nerve Repair following Acute Nerve Injury
Authors: Fliss Ehud , Yanko Ravit , Zaretski Arik , Tulchinsky Roei , Arad Ehud , Kedar Daniel J. , Fliss Dan M. , Gur Eyal 
Published: August 2022

Abstract
Background Acute facial nerve iatrogenic or traumatic injury warrants rapid management with the goal of reestablishing nerve continuity within 72 hours. However, reconstructive efforts should be performed up to 12 months from the time of injury since facial musculature may still be viable and thus facial tone and function may be salvaged.
Methods Data of all patients who underwent facial nerve repair following iatrogenic or traumatic injury were retrospectively collected and assessed. Paralysis etiology, demographics, operative data, postoperative course, and outcome were examined.
Results Twenty patients underwent facial nerve repair during the years 2004 to 2019. Data were available for 16 of them. Iatrogenic injury was the common category (n = 13, 81%) with parotidectomy due to primary parotid gland malignancy being the common surgery (n = 7, 44%). Nerve repair was most commonly performed during the first 72 hours of injury (n = 12, 75%) and most of the patients underwent nerve graft repair (n = 15, 94%). Outcome was available for 12 patients, all of which remained with some degree of facial paresis. Six patients suffered from complete facial paralysis (50%) and three underwent secondary facial reanimation (25%). There were no major operative or postoperative complications.
Conclusion Iatrogenic and traumatic facial nerve injuries are common etiologies of acquired facial paralysis. In such cases, immediate repair should be performed. For patients presenting with facial paralysis following previous surgery or trauma, nerve repair should be considered up to at least 6 months of injury. Longstanding paralysis is best treated with standard facial reanimation procedures.
First-principles study on the electronic structure transition of β-UH3 under high pressure
Authors: Wu Juefei , Yue-Chao Wang , Liu Yu , Sun Bo , Zhao Yanhong , Xian Jiawei , Gao Xingyu , Liu Haifeng , Song Haifeng 
Published: August 2022

We investigate the electronic properties of stable β-UH3 under high pressure up to 75 GPa within the first-principles DFT + U formalism with pressure-dependent U in a self-consistent calculation, and we find an electronic structure transition at about 20 GPa due to the quantum process of localization and itinerancy for partially filled uranium 5f electrons. The electronic structure transition is examined from four perspectives: magnetization, band structure, density of states, and 5f electron energy. On the basis of the density of states of 5f electrons, we propose an order parameter, namely, the 5f electron energy, to quantify the electronic structure transition under pressure. Analogously to the isostructural transition in 3d systems, β-UH3 retains its magnetic order after the electronic structure transition; however, this is not accompanied by volume collapse at the transition point. Our calculation is helpful for understanding the electronic properties of β-UH3 under high pressure.
Waterlogging priming alleviates the oxidative damage, carbohydrate consumption, and yield loss in soybean (
Authors: Agualongo Darwin Alexis Pomagualli , Da-Silva Cristiane Jovelina , Garcia Natália , de Oliveira Fabiane Kletke , Shimoia Eduardo Pereira , Posso Douglas Antônio , de Oliveira Ana Cláudia Barneche , de Oliveira Denise dos Santos Colares , do Amarante Luciano 
Published: August 2022

In this study, we tested whether waterlogging priming at the vegetative stage would mitigate a subsequent waterlogging event at the reproductive stage in soybean [Glycine max (L.) Merr.]. Plants (V3 stage) were subjected to priming for 7 days and then exposed to waterlogging stress for 5 days (R2 stage) with non-primed plants. Roots and leaves were sampled on the fifth day of waterlogging and the second and fifth days of reoxygenation. Overall, priming decreased the H2O2 concentration and lipid peroxidation in roots and leaves during waterlogging and reoxygenation. Priming also decreased the activity of antioxidative enzymes in roots and leaves and increased the foliar concentration of phenols and photosynthetic pigments. Additionally, priming decreased fermentation and alanine aminotransferase activity during waterlogging and reoxygenation. Finally, priming increased the concentration of amino acids, sucrose, and total soluble sugars in roots and leaves during waterlogging and reoxygenation. Thus, primed plants were higher and more productive than non-primed plants. Our study shows that priming alleviates oxidative stress, fermentation, and carbohydrate consumption in parallel to increase the yield of soybean plants exposed to waterlogging and reoxygenation.
An accurate standardization protocol for heating efficiency determination of 3D printed magnetic bone scaffolds
Authors: Makridis A , Kazeli K , Kyriazopoulos P , Maniotis N , Samaras T , Angelakeris M 
Published: August 2022

Abstract
Last decade, three-dimensional (3D) printing technology has emerged as a useful tool for meticulously fabricated scaffolds with high precision and accuracy, resulting in intricately detailed biomimetic 3D structures. To this end, nowadays, magnetic scaffolds are becoming increasingly attractive in tissue engineering, due to their ability not only to promote bone tissue formation, bone repair, and regeneration, but at the same time allow for nanoscale drug delivery. Although there has been a lot of research effort on the fabrication of bone scaffolds in the last few years, their perspectives as multifunctional magnetic hyperthermia agents remain an open issue. This emerging, uninvestigated research field requires a carefully designed framework to produce reliable results. This work focuses on establishing such a framework by proposing a standardization protocol with certain experimental steps for an accurate evaluation of the heating efficiency of the 3D printed magnetic scaffolds bone phantoms. The specific indexes of specific absorption rate and specific loss power are carefully determined and calculated here to enhance the differences in the heating experimental approaches that have been followed until now between magnetic nanoparticles and magnetic bone scaffolds. Meanwhile, the heating evaluation cases that one can find in magnetic hyperthermia are separately defined and analyzed with their suited experimental protocols. Firstly, 3D printed magnetic scaffolds are designed and fabricated. Secondly, they are evaluated as heating carriers. Agarose is exploited here not only as a tissue mimicking phantom, but also as a heat diffusion medium through the scaffold’s pores. A reliable estimation sequence of the heating efficiency, i.e. the specific absorption rate of the magnetic scaffolds, is introduced, analyzed and discussed in conjunction with the specific loss power, which is the respective quantitative index for evaluating the magnetic nanoparticles’ heating efficacy. Finally, this work proposes how the fabrication procedure of the 3D printed scaffolds can be guided by the magnetic particle hyperthermia literature results, as to increase the scaffolds heating efficiency through printing parameters. Consequently, this work deals with the methodology to create a reproducible and accurate protocol for assessing the heating efficiency of magnetic scaffolds serving as bone implants for deep-seated hyperthermia tumor treatment.
In vivo study of beta-glucan-based biogenic synthesis of silver nanocomposite using Schizophyllum commune for wound dressings in a rat burn model
Authors: Safaee-Ardakani Mohammad Reza , Hatamian-Zarmi Ashrafalsadat , Sadat Seyedeh Mahdieh , Alvandi Hale , Ebrahimi-Hosseinzadeh Bahman , Mokhtari-Hosseini Zahra Beagom , Mohammadi Mehrave 
Published: August 2022

Abstract
Bacterial infection is a significant barrier to the natural wound healing process. Silver nanoparticles have antibacterial activity through the destruction of DNA and bacterial membranes. In the present study, a green method was introduced to synthesize silver nanoparticles produced by the Schizophyllum commune. The antibacterial mat loaded with 0.5, 1, and 3% (w/w) of bioactive silver nanoparticles were produced in polyvinyl alcohol (PVA) and schizophyllan (SPG) solution (20:80) with electrospun (PVA/SPG). Then the physicochemical properties of silver nanoparticles and the mats PVA/SPG containing silver nanoparticles were investigated. An animal model also examined antimicrobial activity, cytotoxicity, adhesion, and grade II burn ulcers. The results showed that spherical silver nanoparticles with a diameter of about 40 nm were produced and completely scattered on the nanofibers according to TEM images. A non-covalent interaction was revealed between SPG and AgNPs; the nanofibers were hydrophilic. Nanofibers containing silver nanoparticles had intense antimicrobial activity against E. coli and S. aureus. This inhibition increases with increasing concentration. The cytotoxicity and adhesion results showed that the PVA/SPG-AgNPs 1% sample had the best effect on fibroblast cells. The 1% sample was tested for the animal model, and after 14 days, it had a practical effect on the control. At 14 days post-treatment, a skinny epithelial layer was formed and covered the wound area, and these nanofibers were suitable for wound dressing. Consequently, PVA/SPG-AgNPs nanofibers could be a good option to cover burn wounds by speeding up the wound healing process and treatment costs.
Comparison of the contact and focus variation measurement methods in the process of surface topography evaluation of additively manufactured models with different geometry complexity
Authors: Bazan Anna , Turek Paweł , Przeszłowski Łukasz 
Published: August 2022

Abstract
One of the key measurement parameters of the surface topography is the measurement area. It influences the possibility of using filters separating components of surface texture and thus determines the reliability of the obtained measurement results. The currently applicable standard does not define the size of the measuring area. To determine its size, fractal analysis was carried out in the article. The paper presents research on two types of geometry: simple geometry in the form of cylindrical and spherical surfaces and more complex geometry represented by free surfaces such as crowns and molars of teeth. In the process of making the research models, four 3D printing techniques were used: Fused Deposition Modeling (FDM), Melted and Extruded Modeling (MEM) Fused Filament Fabrication (FFF) and Material Jetting (MJ). 3D measurements of surface texture were made using a contact profilometer and a focus variation microscope. The analysis of topography images and selected parameters of the surface topography showed that the optical method gave better measurement results than the contact method. In the case of models made with the FDM and MEM techniques, similar values of the Sa parameter were obtained. Slightly smaller values of Sa were recorded for FFF models, while the highest for MJ models. Models made using the FFF method were also characterized by the lowest variability of results. Models made using the MJ method were characterized by relatively deep valleys in comparison with the other models, which was reflected in the Ssk and Svk parameters. The valleys counting from the top surface of the specimen were the shallowest for models made with the FFF method. Surfaces with simpler geometry were characterized by smaller variability of parameters values.
Controlling high-speed dry friction through the geometry of micro-patterned asperities
Authors: Florio Catherine S 
Published: August 2022

Abstract
Advances in manufacturing technologies have provided means to create surface textures with regular patterns of uniform asperities, leading to the potential for improved control of friction. In order to design surface topologies that induce desirable tribological effects, an understanding of the influences of the geometric features of asperities on measures of frictional resistance is required. Dynamic elastic-plastic finite element modeling methods, which included material damage and failure, were used to study the interactions of directly modeled 100 micron rib-like asperities on two deformable aluminum blocks. The relationships between the mechanics of the deformation and failure of five unique asperity geometries, the coefficients of static and kinetic friction, and the system energy stored and dissipated were studied under dry, high-load rate conditions, where motion was initiated in under 1 ms and acceleration approached 100 kG. Influences of the geometric features of the asperities were explored using semi-circular, triangular, and square-shaped cross-sectional profiles and evaluated for complex geometries created by combinations of these basic shapes. Static coefficients of friction were found to vary more than two-fold with asperity geometry based on the contact area normal direction. The study found that it was also possible to maintain the static friction coefficient but more than triple the force to initiation motion simply by changing the asperity shape. While kinetic friction coefficients were less influenced by asperity shape for the high-speed conditions studied, the geometric characteristics directed the way an asperity deformed under load and the extent of the material failure during sliding. A more than four-fold variation in energy stored within the system and over an order of magnitude variation in energy dissipated by the system was found for the geometries examined. This study demonstrates the importance of understanding the mechanical behavior of the asperity when designing surface textures to tailor dry, high-speed friction.
A novel IgG Fc by computer-aided design enhances heavy-chain heterodimerization in bi- or trispecific antibodies
Authors: Wang Bo , Lin Jun , Hoag Matthew R , Wright Meredith , Ma Mingjun , Cai Wenyan , Gallolu Kankanamalage Sachith , Liu Yue 
Published: August 2022

Abstract
The classical `knob-into-holes' (KIH) strategy (knob(T366Y)/hole (Y407T)) has successfully enhanced the heterodimerization of a bispecific antibody (BsAb) resulting in heterodimer formation up to 92% of protein A (ProA)-purified protein pool. However, it does not show high efficiency for every BsAb. KIH was initially applied to a CD20/CD3 BsAb. After in silico modeling, two additional new mutations, S354Y in knob-heavy chain (HC) and Q347E in hole-HC, together with KIH named `ETYY', were introduced in the Fc. The CD20/CD3 BsAb hybrid only represented ~ 50% of the ProA-purified protein pool when KIH was applied. With ETYY, the percentage of CD20/CD3 hybrid increased to 93.8%. CD20/CD3-v4b (containing ETYY) retains the original activity of the BsAb at both Fab and Fc regions, and also shows good developability. These results indicate that the computer-aided novel ETYY design has the potential to improve the development of next-generation BsAbs with higher yields and simpler purification.
Implementation of an integrated pharmacist collaborative care model in specialty disease state clinics
Authors: Stroedecke Natasha , Lee Jenna , Stutsky Martha , Boothe Kimberly , Tong Kimhouy , Luon Steph , Sawant Vinay , Renauer Marie 
Published: August 2022

Abstract

Purpose
To expand health-system specialty pharmacy (HSSP) clinical continuity by implementing a specialty integrated model for clinical services in target sites.

Summary
After evaluation of baseline clinical continuity and institutional goals, select clinics were identified as target sites to which to expand this integrated approach of a medication management clinic (MMC). In this MMC model, the key steps included engaging stakeholders, workflow training, optimization of the electronic health record, service evaluation, compliance with regulatory standards, and development of marketing strategies. The initial focus was development of innovative collaborative practice agreements (CPAs) to expand the scope of ambulatory care pharmacists’ practice. Analysis of existing specialty and ambulatory workflows and technology was completed before development of the integrated workflow. Existing credentialing policies were updated to support expanded practices, and marketing collaterals were developed to support growth of pharmacy referrals. Meetings with stakeholders took place to ensure smooth transitions into integrated areas. Primary endpoints included clinical continuity, as determined by prescription orders placed within the health system sent to the HSSP, and number of signed referrals to MMC. Secondary endpoints included disease state–specific clinical outcomes as well as overall outcomes such as medication adherence, laboratory test adherence, immunization rates, and patient and clinician satisfaction. An MMC model was successfully implemented in 5 target specialty practices. Specialty clinic CPAs were developed for rheumatology and digestive health (including viral hepatitis). Since implementation, clinical continuity increased 23% and referrals exceeded the target at 165%. Data on secondary endpoints are currently being collected to evaluate quality of pharmacy services. Pharmacy services have enhanced patient care and received positive feedback from clinicians.

Conclusion
Expansion of integrated decentralized pharmacists into target practices has increased clinical continuity and the number of pharmacist referrals. Clinicians have regarded pharmacists as vital members of the team. Creation of additional specialty CPAs will be needed to support further growth in other clinics.
Transcriptomic and histochemical analysis reveal the complex regulatory networks in equine chorioallantois during spontaneous term labor
Authors: El-Sheikh Ali Hossam , Scoggin Kirsten , Murase Harutaka , Norris Jamie , Menarim Bruno , Dini Pouya , Ball Barry 
Published: August 2022

AbstractThe equine chorioallantois (CA) undergoes complex physical and biochemical changes during labor. However, the molecular mechanisms controlling these changes are still unclear. Therefore, the current study aimed to characterize the transcriptome of equine CA during spontaneous labor and compare it with that of normal preterm CA. Placental samples were collected postpartum from mares with normal term labor (TL group, n = 4) and from preterm not in labor mares (330 days GA; PTNL group, n = 4). Our study identified 4137 differentially expressed genes (1820 upregulated and 2317 downregulated) in CA during TL as compared with PTNL. TL was associated with the upregulation of several proinflammatory mediators (MHC-I, MHC-II, NLRP3, CXCL8, and MIF). Also, TL was associated with the upregulation of matrix metalloproteinase (MMP1, MMP2, MMP3, and MMP9) with subsequent extracellular matrix degradation and apoptosis, as reflected by upregulation of several apoptosis-related genes (ATF3, ATF4, FAS, FOS, and BIRC3). In addition, TL was associated with downregulation of 21 transcripts coding for collagens. The upregulation of proteases, along with the downregulation of collagens, is believed to be implicated in separation and rupture of the CA during TL. Additionally, TL was associated with downregulation of transcripts coding for proteins essential for progestin synthesis (SRD5A1 and AKR1C1) and angiogenesis (VEGFA and RTL1), as well as upregulation of prostaglandin synthesis-related genes (PTGS2 and PTGES), which could reflect the physiological switch in placental endocrinology and function during TL. In conclusion, our findings revealed the equine CA gene expression signature in spontaneous labor at term, which improves our understanding of the molecular mechanisms triggering labor.
Disrupted dynamic network reconfiguration of the brain functional networks of individuals with autism spectrum disorder
Authors: Wang Min , Wang Lingxiao , Yang Bo , Yuan Lixia , Wang Xiuqin , Potenza Marc N , Dong Guang Heng 
Published: August 2022

AbstractHuman and animal studies on brain functions in subjects with autism spectrum disorder have confirmed the aberrant organization of functional networks. However, little is known about the neural features underlying these impairments. Using community structure analyses (recruitment and integration), the current study explored the functional network features of individuals with autism spectrum disorder from one database (101 individuals with autism spectrum disorder and 120 healthy controls) and tested the replicability in an independent database (50 individuals with autism spectrum disorder and 74 healthy controls). Additionally, the study divided subjects into different age groups and tested the features in different subgroups. As for recruitment, subjects with autism spectrum disorder had lower coefficients in the default mode network and basal ganglia network than healthy controls. The integration results showed that subjects with autism spectrum disorder had a lower coefficient than healthy controls in the default mode network–medial frontal network and basal ganglia network–limbic networks. The results for the default mode network were mostly replicated in the independent database, but the results for the basal ganglia network were not. The results for different age groups were also analysed, and the replicability was tested in different databases. The lower recruitment in subjects with autism spectrum disorder suggests that they are less efficient at engaging these networks when performing relevant tasks. The lower integration results suggest impaired flexibility in cognitive functions in individuals with autism spectrum disorder. All these findings might explain why subjects with autism spectrum disorder show impaired brain networks and have important therapeutic implications for developing potentially effective interventions.