bluuebunny/crossref_metadata_2025_split huggingface dataset:
The in vitro effects of selected substances and nanomaterials against Diaporthe eres, Diplodia seriata and Eutypa lata
Authors: Štůsková Kateřina , Pečenka Jakub , Tekielska Dorota Anna , Špetík Milan , Bytešníková Zuzana , Švec Pavel , Ondreáš František , Ridošková Andrea , Richtera Lukáš , Adam Vojtěch , Eichmeier Aleš 
Published: November 2022

AbstractGrapevine trunk pathogens (GTPs) cause serious damage to grapevines and have significant economic impacts. There is no effective protection against grapevine trunk diseases. Newly designed AgSe nanoparticles (NPs) and CuSe NPs, single‐element Ag NPs, Cu NPs, Se NPs and selected chemicals or chemical agents such as sodium arsenite, 8‐hydroxyquinoline, silver nitrate, colloidal silver, Altron Silver fertilizer and silver thiosulfate complex (NH4)3/Ag(S2O3)2 were tested in vitro against two serious GTPs Diaporthe eres, Eutypa lata and Diplodia seriata isolated from walnut. The most significant inhibition of fungal growth was observed for silver nitrate and AgSe NPs, which showed the highest level of half the maximum effective EC50 concentration with the lowest concentrations. In the case of silver nitrate at a concentration of 1000 mg L−1, 79% inhibition of mycelial growth was observed for the pathogen E. lata, 48% for D. seriata and 54% for D. eres. AgSe NPs, in which the concentration of silver is 2588 mg L−1 and that of selenium is 902 mg L−1, showed 68% inhibition of mycelial growth in the pathogen E. lata, 54% in D. seriata and 58% in D. eres.
Combined plasma levels of IL‐10 and testosterone, but not soluble HLA‐G5, predict the risk of death in COVID‐19 patients
Authors: Amodio Giada , Capogrosso Paolo , Pontillo Marina , Tassara Michela , Boeri Luca , Carenzi Cristina , Cignoli Daniele , Ferrara Anna Maria , Ramirez Giuseppe A. , Tresoldi Cristina , Locatelli Massimo , Santoleri Luca , Castagna Antonella , Zangrillo Alberto , De Cobelli Francesco , Tresoldi Moreno , Landoni Giovanni , Rovere‐Querini Patrizia , Ciceri Fabio , Montorsi Francesco , Salonia Andrea , Gregori Silvia 
Published: November 2022

AbstractBackgroundThe identification of biomarkers correlated with coronavirus disease 2019 (COVID‐19) outcomes is a relevant need for clinical management. Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection is characterized by elevated interleukin (IL)‐6, IL‐10, HLA‐G, and impaired testosterone production.ObjectivesWe aimed at defining the combined impact of sex hormones, interleukin‐10, and HLA‐G on COVID‐19 pathophysiology and their relationship in male patients.Materials and methodsWe measured by chemiluminescence immunoassay, electrochemiluminescent assays, and enzyme‐linked immunosorbent assay circulating total testosterone, 17β‐estradiol (E2), IL‐10, and ‐HLAG5 as well as SARS‐CoV‐2 S1/S2 Immunoglobulin G from 292 healthy controls and 111 COVID‐19 patients with different disease severity at hospital admission, and in 53 COVID‐19 patients at 7‐month follow‐up.Results and discussionWe found significantly higher levels of IL‐10, HLA‐G, and E2 in COVID‐19 patients compared to healthy controls and an inverse correlation between IL‐10 and testosterone, with IL‐10, progressively increasing and testosterone progressively decreasing with disease severity. This correlation was lost at the 7‐month follow‐up. The risk of death in COVID‐19 patients with low testosterone increased in the presence of high IL‐10. A negative correlation between SARS‐CoV‐2 Immunoglobulin G and HLA‐G or IL‐10 at hospitalization was observed. At the 7‐month follow‐up, IL‐10 and testosterone normalized, and HLA‐G decreased.ConclusionOur findings indicate that combined evaluation of IL‐10 and testosterone predicts the risk of death in men with COVID‐19 and support the hypothesis that IL‐10 fails to suppress excessive inflammation by promoting viral spreading.
Outcome after veno‐arterial extracorporeal membrane oxygenation in elderly patients: A 14‐year single‐center experience
Authors: Provaznik Zdenek , Philipp Alois , Müller Thomas , Kostiantyn Kozakov , Lunz Dirk , Schmid Christof , Floerchinger Bernhard 
Published: November 2022

AbstractBackgroundUse of veno‐arterial extracorporeal membrane oxygenation (VA‐ECMO) in elderly patients is controversial because of presumed poor outcome. Our primary aim was to determine the influence of advanced age on short‐ and long‐term outcome; the secondary aim was to analyze risk factors for impaired outcome.MethodsBetween January 2006 and June 2020, 645 patients underwent VA‐ECMO implantation in our department. The patients were categorized into four groups:<50, 50–59.9, 60–69.9 and ≥70 years old. Data were retrospectively analyzed for short‐ and long‐term outcome. Risk factors for in‐hospital mortality and mortality during follow‐up were assessed using multivariate regression analysis.ResultsVA‐ECMO support duration was comparable in all age groups (median 3 days). Weaning rates were 60.8%/n = 104 (<50 years), 51.4%/n = 90 (50–59.9 years), 58.8%/n = 107 (60–69.9), and 67.5%/n = 79 (≥70, p = 0.048). Hospital mortality was highest in the patients aged 50–59.9 years (68%/n = 119), but not in the elderly patients (60–69.9, ≥70:62.1%/n = 113, 58,1%/n = 68). At discharge, the cerebral performance category scores were superior in the patients <50 years. Multivariate logistic regression analysis revealed chronic kidney failure requiring hemodialysis, duration of cardiopulmonary resuscitation, and elevated blood lactate levels before VA‐ECMO, but not age as predictors of in‐hospital mortality. Cox's regression disclosed age as relevant risk factor for death during follow‐up. The patients' physical ability was comparable in all age groups.ConclusionVA‐ECMO support should not be declined in patients only because of advanced age. Mortality and neurological status at hospital discharge and during follow‐up were comparable in all age groups.
Randomised clinical trial: effects of MD‐7246 on irritable bowel syndrome with diarrhoea
Authors: Lembo Anthony , Kuo Braden , Boinpally Ramesh , Li Ella , Mallick Madhuja , Bochenek Wieslaw , Bartolini Wilmin 
Published: November 2022

SummaryBackgroundMD‐7246, a delayed‐release formulation of linaclotide, is designed to target the ileocaecal junction and caecum with the aim of relieving abdominal pain independently of bowel function.AimsTo evaluate the efficacy, safety and dose–response of MD‐7246 in patients with irritable bowel syndrome with diarrhoea (IBS‐D).MethodsA randomised, double‐blind, phase 2 clinical trial enrolled adult patients with IBS‐D (Rome IV criteria). Patients were randomised to placebo or once‐daily oral MD‐7246 300, 600 or 1200 μg for 12 weeks. Abdominal and bowel symptoms were assessed daily. Key efficacy endpoints were change from baseline in abdominal pain and responder rates for a 30% reduction in abdominal pain in 6/12 weeks. Additional abdominal pain responder and exploratory bowel function endpoints were also assessed.ResultsAmong the 388 randomised patients, there was no significant difference in mean change from baseline in abdominal pain between the MD‐7246 300 μg, 600 μg and 1200 μg groups and placebo (−1.93, −1.58, −1.95 and − 2.01, respectively; p > 0.05 for each group vs placebo). The abdominal pain responder rates in the MD‐7246 groups were similar to or lower than those in the placebo group. All doses of MD‐7246 had a minimal effect on bowel function and were generally well tolerated.ConclusionsMD‐7246 at the doses studied did not improve abdominal pain relative to placebo in an IBS‐D patient population. Similarly, most additional efficacy endpoints showed no improvement with MD‐7246 relative to placebo.
In vitro effects of 2‐methyl‐3‐propylbutane‐1,4‐diol purified from Alstonia boonei on erythrocyte membrane stabilization and mitochondrial membrane permeabilization
Authors: Olanlokun John Oludele , Oyebode Olubukola Titilope , Popoola David , Bodede Olusola , Idowu Thomas Oyebode , Moodley Roshila , Olorunsogo Olufunso Olabode 
Published: November 2022

AbstractA recent review on the ethnomedicinal, chemical, pharmacological, and toxicological properties of Alstonia boonei revealed the plant's potential in the treatment and management of a range of diseases. However, most of these pharmacological effects are only traceable to the crude form of the plant extract and not specific natural products. Phytochemical investigation of the methanol fraction of the methanol extract of the stem‐bark of Alstonia boonei led to the isolation and identification of 2‐methyl‐3‐propylbutane‐1,4‐diol. The structures were elucidated by the application of 1D‐, and 2D‐NMR spectroscopic analyses and by comparison with literature data. In this study, the membrane stabilizing activity, mitochondrial membrane permeability transition pore opening, cytochrome c release, mitochondrial ATPase activity, and prevention of mitochondrial lipid peroxidation activity of 2‐methyl‐3‐propylbutane‐1,4‐diol (MPBD) isolated from A. boonei were determined. The results showed that MPBD significantly (p < .05) prevented peroxidation of mitochondrial membrane lipids and hemolysis using both the heat‐induced and hypotonic solution‐induced membrane stabilization assays. On the contrary, the compound caused large amplitude swelling of rat liver mitochondria in the absence of calcium, significant (p < .05) cytochrome c release and enhancement of mitochondrial ATPase activity in vitro. Our findings suggest that MPBD showed characteristic biological properties useful in modulating cell death.
Reliability and validity of the Dutch‐language version of the Viking Speech Scale in children with cerebral palsy
Authors: Spaans Irene E. M. , Geytenbeek Joke J. M. , Vaillant Emma , de Kleijn Maaike A. M. C. , Buizer Annemieke I. , Pennington Lindsay 
Published: November 2022

AbstractPurposeThe Viking Speech Scale is used to classify speech performance in children with cerebral palsy (CP). A Dutch‐language version (VSS‐NL) has recently become available. This study aimed to determine the reliability and validity of the VSS‐NL and the association with motor type of CP, Gross Motor Function Classification System (GMFCS), Manual Ability Classification System (MACS) and Communication Function Classification System (CFCS).MethodsA total of 90 children with CP, recruited throughout the Netherlands, took part in the study. VSS‐NL classifications by speech and language therapists unfamiliar (SLT1) and familiar (SLT2) with the child, parents and physicians were compared. Convergent and discriminant validity were determined with the Intelligibility in Context Scale‐Dutch (ICS‐NL) and the Computer Based Instrument for Low Motor Language Testing (C‐BiLLT). Inter‐ and intrarater reliability were determined by weighted Kappa (ƙw). Validity and associations between VSS‐NL and GMFCS, MACS and CFCS were determined with Spearman's coefficient. Association between VSS‐NL and motor type of CP was determined with Fisher's exact test.ResultsInterrater reliability was excellent between SLT1‐SLT2 (ƙw = 0.93, 95% confidence interval [CI] 0.87–0.99), good between SLT1‐parent (ƙw = 0.71, 95% CI 0.60–0.83), SLT1‐physician (ƙw = 0.70, 95% CI 0.58–0.81), SLT2‐parent (ƙw = 0.71, 95% CI 0.57–0.84), SLT2‐physician (ƙw = 0.73, 95% CI 0.62–0.85) and parent‐physician (ƙ = 0.72, 95% CI 0.60–0.85). Intrarater reliability was excellent for SLTs familiar and unfamiliar to the child (ƙw = 1.00, 95% CI 1.00–1.00), and very good for physicians (ƙw = 0.89, 95% CI 0.75–1.00) and parents (ƙw = 0.72, 95% CI 0.62–1.00).Convergent validity was very strong (r = −0.81, p < 0.001) and discriminant validity moderate (r = −0.56, p < 0.001). Association with motor type of CP was significant (χ2 = 27.558, p < 0.001) and strong with GMFCS (r = 0.62, p < 0.001), MACS (r = 0.63, p < 0.01) and CFCS (r = 0.69, p < 0.001).ConclusionThe VSS‐NL is a reliable and valid system to classify speech performance in children with cerebral palsy. Classifications can be performed by SLTs, parents and physicians.
Sulphur flow in soil–plant system with long‐term agricultural practices on a Vertisol
Authors: Ghosh Deblina , Murmu Sidhu , Adhikary Samrat , Mukherjee Aritra Kumar , Mal Sudipa , Khanam Rubina , Pawar Archana , Pharande Ashok Laxman , More Nilkanth B. , Mandal Biswapati 
Published: November 2022

AbstractSulphur (S) deficiency is widespread in agricultural fields across the globe. Its flow in the soil–plant system under different management practices is too poorly understood to strategize S fertilisation with increased efficiency. Accordingly, an attempt was made to elucidate the pathways of S flow in soils and to plants under organic, inorganic, and integrated nitrogen (N) management practices using a 30‐year‐old long‐term field experiment planted with sorghum in the semi‐arid tropics. Partitioning soil S into pools, we observed that exchangeable (4.2%) and adsorbed (4.6%) pools constituted a small part of total soil S but contributed mainly to S nutrition (46% and 33%) of sorghum; it also contributed to the variability of S extractable by six commonly used extractants. Using multiple criteria, the superiority of monocalcium phosphate as an extractant for assessment of S in Vertisols was established. Among the N management practices, integrated N management performed better in supplying S in soils and to sorghum, and excelled, both in retention (35.9% of applied) and utilisation (13%) of S, curbing its losses (51.1%) from the system, over the organics (26%, 8.4%, and 65.6%, respectively) and inorganic (11.6%, 7.1%, and 81.3%, respectively) management practices. The integrated management practices with farmyard manure (FYM) excelled in improving S in soils, including economics. Out of the organics, FYM was more effective in improving S availability in soils and its nutrition of the crop over the crop residue, and green manure. Changes in organic C, and oxides and hydroxides of Fe and Al in soils are the keys governing the flows of S in the soil–plant system. A detailed pathway of the increased efficacy of S fertilisation upon conjoint application of N sources over the others was highlighted for adoption in Vertisols of semi‐arid tropics.Highlights
Inter‐relationship between pools and extractants of S were elucidated.
Organic C and sesquioxides played a key role in S transformation in soils.
Monocalcium phosphate was the most sensitive extractant for S in soils.
Sixty two percent of the applied S was lost, 28% retained and 10% utilised.
Random regression models using B‐splines functions provide more accurate genomic breeding values for milk yield and lactation persistence in Murrah buffaloes
Authors: Silva Alessandra A. , Brito Luiz F. , Silva Delvan A. , Lazaro Sirlene F. , Silveira Karina R. , Stefani Gabriela , Tonhati Humberto 
Published: November 2022

AbstractThere is a great worldwide demand for cheese made with buffalo milk, due to its flavour and nutritional properties. In this context, there is a need for increasing the efficiency of buffalo milk production (including lactation persistence), which can be achieved through genomic selection. The most used methods for the genetic evaluation of longitudinal data, such as milk‐related traits, are based on random regression models (RRM). The choice of the best covariance functions and polynomial order for modelling the random effects is an important step to properly fit RRM. To our best knowledge, there are no studies evaluating the impact of the order and covariance function (Legendre polynomials—LEG and B‐splines—BSP) used to fit RRM for genomic prediction of breeding values in dairy buffaloes. Therefore, the main objectives of this study were to estimate variance components and evaluate the performance of LEG and BSP functions of different orders on the predictive ability of genomic breeding values for the first three lactations of milk yield (MY1, MY2, and MY3) and lactation persistence (LP1, LP2, and LP3) of Brazilian Murrah. Twenty‐two models for each lactation were contrasted based on goodness of fit, genetic parameter estimates, and predictive ability. Overall, the models of higher orders of LEG or BSP had a better performance based on the deviance information criterion (DIC). The daily heritability estimates ranged from 0.01 to 0.30 for MY1, 0.08 to 0.42 for MY2, and from 0.05 to 0.47 for MY3. For lactation persistence (LP), the heritability estimates ranged from 0.09 to 0.32 for LP1, from 0.15 to 0.33 for LP2, and from 0.06 to 0.32 for LP3. In general, the curves plotted for variance components and heritability estimates based on BSP models presented lower oscillation along the lactation trajectory. Similar predictive ability was observed among the models. Considering a balance between the complexity of the model, goodness of fit, and credibility of the results, RRM using quadratic B‐splines functions based on four or five segments to model the systematic, additive genetic, and permanent environment curves provide better fit with no significant differences between genetic variances estimates, heritabilities, and predictive ability for the genomic evaluation of dairy buffaloes.
Systematic review of dyadic psychoeducational programs for persons with dementia and their family caregivers
Authors: Ghosh Manonita , Dunham Melissa , O'Connell Beverly 
Published: November 2022

AbstractAims and ObjectivesSynthesising evidence for effects of dyadic psychoeducational support programs on both people with dementia and their caregivers' health and well‐being.BackgroundThere is an increasing need for psychoeducational support programs for people with dementia and their caregivers; therefore, it is important to identify the benefits and practical implications of the programs on the dyads.MethodsGuided by Joanna Briggs Institute (JBI) methodology, and Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) framework, a systematic search of literature was conducted on dyadic psychoeducational programs published in English between January 2012 and January 2021 from four electronic databases.ResultsTwenty‐four studies evaluating 27 psychoeducational programmes were included in this review. Programs varied in activity types, intensity and duration. Outcome effects on people with dementia were grouped into seven categories: quality of life, cognitive function, psychological and mental health, physical health, changed behaviours, communication and relationship, institutionalisation or mortality. Outcome effects on caregivers were grouped into six: psychological and mental health, quality of life, impact of caregiving, communication and relationship, physical health, and competency. Dyadic psychoeducational programs which were goal oriented and tailored to address individual needs had consistent benefits on various aspects of health and quality of life for the dyads.ConclusionsMulticomponent psychoeducational support programs combined with addressing individual needs, identifying goals and providing support to attain specific outcomes are recommended. Given the progressive deterioration of people with dementia, and the increased needs for homecare by family members, delivering long‐term, support programs are recommended to maintain the positive effects on the dyads.Relevance to Clinical PracticeThe findings contribute to dementia‐care provision and policy making and inform the development of person‐centred interventions and governance.Patient or Public ContributionThis systematic review was a part of a larger service evaluation project which involved a dementia consumer advisory group.
e‐Learning interventions for nurses to prevent venous thromboembolism in patients: A realist review
Authors: Dyke Ellen V. , Jauncey‐Cooke Jacqueline , Johnston Amy N. B. 
Published: November 2022

AbstractAimTo synthesise published literature exploring online venous thromboembolism (VTE) prevention education programmes to identify, test and refine a program theory supporting translation of knowledge into practice for registered nurses.BackgroundVenous thromboembolism is a leading cause of preventable morbidity and mortality in hospitalised patients. Successful implementation of prevention strategies can be impeded by a lack of education and translation of knowledge to practice. Continuing professional development programs using e‐learning platforms are increasingly common, however, there is little published literature exploring effective translation of such education to practice.DesignRealist Review.MethodsSearches were conducted in seven healthcare databases prior to July 2020 and updated in March 2022. Synthesis was informed by the unified theory of acceptance and use of technology (UTAUT) model and followed stages of a realist review outlined by Pawson et al. The results were reported according to the RAMESES publication standards.ResultsEight context‐mechanism‐outcome combinations were identified to explain engagement, completion and adoption of VTE e‐learning into practice. Mechanisms included valuing content as relevant to practice, having confidence in the ability to use the technology and empowerment to enact change based on learning. Contextual factors that may constrain the completion of learning include the support of managers and organisations through time, and the availability of resources.ConclusionTranslation of e‐learning is critical to ensure clinical excellence. This realist review demonstrated the varying mechanisms contributing to engagement, completion and adoption of learning. These illustrate the complex nature of education for professional development. It demonstrates that the UTAUT model is suitable for guiding the design, implementation and adoption of e‐learning programmes.Relevance to clinical practiceThis review used a common clinical practice (VTE) to provide a program theory that can guide clinical educators to understand mechanisms which can facilitate engagement with, completion and adoption of e‐learning into practice by nurses.No patient or public contributionThe focus of this realist review was on e‐learning for registered nurses. As such, no patient or public contribution was sought or given in the development, progress and writing of the submitted manuscript.
Out of the Qinghai‐Tibet plateau: Genomic biogeography of the alpine monospecific genus Megadenia (Biscutelleae, Brassicaceae)
Authors: Yang Wenjie , Feng Landi , Jiao Pengfei , Xiang Ling , Yang Luobai , Olonova Marina V. , Chepinoga Victor V. , Al‐Shehbaz Ihsan A. , Liu Jianquan , Hu Quanjun 
Published: November 2022

AbstractNumerous high‐elevation alpine plants of the Qinghai‐Tibet Plateau (QTP) also have disjunct distribution in adjacent low‐altitude mountains. The out‐of‐QTP versus into‐the‐QTP hypothesis of alpine plants provide strong evidence for the highly disputed assumption of the massive ice sheet developed in the central plateau during the Last Glacial Maximum (LGM). In this study, we sequenced the genomes of most known populations of Megadenia, a monospecific alpine genus of Brassicaceae distributed primarily in the QTP, though rarely found in adjacent low‐elevation mountains of north China and Russia (NC‐R). All sequenced samples clustered into four geographic genetic groups: one pair was in the QTP and another was in NC‐R. The latter pair is nested within the former, and these findings support the out‐of‐QTP hypothesis. Dating the four genetic groups and niche distribution suggested that Megadenia migrated out of the QTP to adjacent regions during the LGM. The NC‐R group showed a decrease in the effective population sizes. In addition, the genes with high genetic divergences in the QTP group were mainly involved in habitat adaptations during low‐altitude colonization. These findings reject the hypothesis of development massive ice sheets, and support glacial survival of alpine plants within, as well as further migration out of, the QTP.