bluuebunny/crossref_metadata_2025_split huggingface dataset:
Atomic-level characterization of the conformational transition pathways in SARS-CoV-1 and SARS-CoV-2 spike proteins
Authors: Ogden Dylan S , Moradi Mahmoud 
Published: November 2022

AbstractSevere acute respiratory syndrome (SARS) coronaviruses 1 and 2 (SARS-CoV-1 and SARS-CoV-2) derive transmissibility from spike protein activation in the receptor binding domain (RBD) and binding to the host cell angiotensin converting enzyme 2 (ACE2). However, the mechanistic details that describe the large-scale conformational changes associated with spike protein activation or deactivation are still somewhat unknown. Here, we have employed an extensive set of nonequilibrium all-atom molecular dynamics (MD) simulations, utilizing a novel protocol, for the SARS-CoV-1 (CoV-1) and SARS-CoV-2 (CoV-2) prefusion spike proteins in order to characterize the conformational pathways associated with the active-to-inactive transition. Our results indicate that both CoV-1 and CoV-2 spike proteins undergo conformational transitions along pathways unique to each protein. We have identified a number of key residues that form various inter-domain saltbridges, suggesting a multi-stage conformational change along the pathways. We have also constructed the free energy profiles along the transition pathways for both CoV-1 and CoV-2 spike proteins. The CoV-2 spike protein must overcome larger free energy barriers to undergo conformational changes towards protein activation or deactivation, when compared to CoV-1.
Calpain-2 mediates SARS-CoV-2 entry and represents a therapeutic target
Authors: Zeng Qiru , Antia Avan , Puray-Chavez Maritza , Kutluay Sebla B. , Ding Siyuan 
Published: November 2022

ABSTRACTSince the beginning of the coronavirus disease 2019 (COVID-19) pandemic, much effort has been dedicated to identifying effective antivirals against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A number of calpain inhibitors show excellent antiviral activities against SARS-CoV-2 by targeting the viral main protease (Mpro), which plays an essential role in processing viral polyproteins. In this study, we found that calpain inhibitors potently inhibited the infection of a chimeric vesicular stomatitis virus (VSV) encoding the SARS-CoV-2 spike protein, but not Mpro. In contrast, calpain inhibitors did not exhibit antiviral activities towards the wild-type VSV with its native glycoprotein. Genetic knockout of calpain-2 by CRISPR/Cas9 conferred resistance of the host cells to the chimeric VSV-SARS-CoV-2 virus and a clinical isolate of wild-type SARS-CoV-2. Mechanistically, calpain-2 facilitates SARS-CoV-2 spike protein-mediated cell attachment by positively regulating the cell surface levels of ACE2. These results highlight an Mpro-independent pathway targeted by calpain inhibitors for efficient viral inhibition. We also identify calpain-2 as a novel host factor and a potential therapeutic target responsible for SARS-CoV-2 infection at the entry step.
Data mining and experimental approaches to identify combination of natural herbs against bacterial infections
Authors: Mittal Ekansh , Duncan Susan , Chamberlin Steven 
Published: November 2022

AbstractVarious studies have identified that natural herbs can be repurposed to treat infectious and bacterial diseases. The purpose of this study is first to test the medicinal value of five herbs including asafoetida, cumin, fenugreek, neem, and turmeric as single agent and in pairs using the bacterial zone of inhibition assay. Second, we used target and network analyses to predict the best combinations. We found that all the herbs as single agent were effective against bacterial infection in the following descending order of efficacy: cumin > turmeric > neem > fenugreek > asafoetida as compared to vehicle (ethanol) treated control. Among all the tested combinations the turmeric and fenugreek combination had the best efficacy in inhibiting the bacterial growth. Next to understand the mechanism of action and to predict the effective combinations among available herbs, we used a data mining and computational analysis approach. Using NPASS, BindingDB, and pathway analysis tools, we identified the bioactive compounds for each herb, then identified the targets for each bioactive compound, and then identified associated pathways for these targets. Then we measured the target/pathway overlap for each herb and identified that the most effective combinations were those which have non-overlapping targets/pathways. For example, we showed as a proof-of-concept that turmeric and fenugreek have the least overlapping targets/pathways and thus is most effective in inhibiting bacteria growth. Our approach is applicable to treat bacterial infections and other human diseases such as cancer. Overall, the computational prediction along with experimental validation can help identify novel combinations that have significant antibacterial activity and may help prevent drug-resistant microbial diseases in human and plants.
Self-regulation of PIN1-driven auxin transport by cell surface-based auxin signaling inArabidopsis
Authors: Wang Jiacheng , Chang Mingzeng , Huang Rongfeng , Gallei Michelle , Friml Jiřn , Yu Yongqiang , Wen Mingzhang , Yang Zhenbiao , Xu Tongda 
Published: November 2022

AbstractPIN-FORMED (PIN)-driven auxin transport contributes to establishing and maintaining a dynamic auxin concentration gradient alongside plant tissues, which drives the majority of developmental processes in plants. To maintain developmental plasticity in ever-changing environments, plants have evolved self-organizing feedback machinery between auxin signaling and its transport, which has been shown to play essential roles in many fundamental plant processes. However, the molecular mechanism behind this mutual regulation has not yet been clarified. Here, we identified a cell surface-triggered auxin signaling that regulates the PIN1-mediated auxin efflux and further developmental patterning inArabidopsis. Auxin was able to stimulate PIN1 phosphorylation in plants through transmembrane kinases (TMKs), key components in auxin signaling, at the plasma membrane. TMK1 and TMK4 directly interacted with and phosphorylated PIN1 and functioned redundantly in the regulation of PIN1 polarity in plant cells. The phosphorylation sites in PIN1 proteins, targeted by both auxin and TMKs, were required for PIN1 trafficking and polarity, which further controlled auxin responses and downstream developmental patterning inArabidopsis. Therefore, our findings provide a direct mechanism for the self-regulation between auxin signaling and transport that drives the auxin flows and proper development in plants.
Risk assessment model for halal supply chain using an integrated approach of IFN and D number
Authors: Khan Shahbaz , Haleem Abid , Khan Mohd Imran 
Published: November 2022

PurposeHalal integrity assurance is the primary objective of Halal supply chain management. Several halal-related risks are present that have the potential to breach halal integrity. Therefore, this study aims to develop the framework for the assessment of halal-related risk from a supply chain perspective.Design/methodology/approachRisk related to halal is identified through the combined approach of the systematic literature review and experts’ input. Further, these risks are assessed using the integrated approach of intuitionistic fuzzy number (IFN) and D-number based on their severity score. This integrated approach can handle fuzziness, inconsistency and incomplete information that are present in the expert’s input.FindingsEighteen significant risks related to halal are identified and grouped into four categories. These risks are further prioritised based on their severity score and classified as “high priority risk” or “low priority risks”. The findings of the study suggests that raw material status, processing methods, the wholesomeness of raw materials and common facilities for halal and non-halal products are more severe risks.Research limitations/implicationsThis study only focusses on halal-related risks and does not capture the other types of risks occurring in the supply chain. Risks related to halal supply chain management are not considered in this study. Prioritisation of the risks is based on the expert’s input which can be biased to the experts' background.Practical implicationsThe proposed risk assessment framework is beneficial for risk managers to assess the halal related risks and develop their mitigation strategies accordingly. Furthermore, the prioritisation of the risks also assists managers in the optimal utilisation of resources to mitigate high-priority risks.Originality/valueThis study provides significant risks related to halal integrity, therefore helping in a better understanding of the halal supply chain. To the best of the authors' knowledge, this is the first comprehensive study for developing a risk assessment model for the halal supply chain.
“What they allow us to learn”: exploring how white English teachers cultivate students’ critical literacies through curriculum and pedagogy
Authors: Kelly Lauren Leigh 
Published: November 2022

PurposeThis qualitative research study examines classroom observations and transcripts, teacher and student interviews and student writing to investigate how white English teachers can cultivate students’ critical literacies regarding race and oppression through classroom literature. As research and practice in the field of critical literacy has yet to effectively center black, indigenous, and people of color (BIPOC) lives and histories, this study aims to expand on existing critical literacy research by examining how literature teachers disrupt the perpetuation of whiteness through literature instruction that explicitly grapples with race and structures of oppression.Design/methodology/approachThis research examines the pedagogical practices of two white English teachers through a yearlong investigation of classroom instruction and curriculum in an urban high school in a large Northeastern city. The overarching question of this study asks, how do white English teachers cultivate students’ critical literacies regarding race and social justice through classroom literature? Additional questions that guided this study are: How do students in these classes learn about structures of oppression? What language is used in these classrooms to discuss ideas about power? What texts and materials do these teachers use to engage students in critical literacy practices?FindingsThe findings of this study provide insight as to how white English teachers can foster students’ critical literacy development regarding race and oppression through their pedagogy and curriculum. The two teachers’ introduction of critical language and frameworks in the classroom supported students’ ability to critically engage with classroom literature and with their own social worlds. In addition, these teachers’ practices emphasize the need for white teachers to decenter their own knowledge and identities to effectively foster students' critical and sociopolitical development.Originality/valueThis research responds to McLeanet al.’s (2021) call for a disruption of the “perpetuation of Eurocentric, hegemonic perspectives by white scholars” in the field by centering race in approaches to critical literacy development in the classroom. By analyzing data from classrooms in the same school with distinct curricular approaches, this study examines not only what but also how educators are teaching in classrooms designed to cultivate students’ critical and sociopolitical development through English Language Arts. This study offers hope for developing critical and culturally sustaining pedagogies among non-BIPOC educators who teach Black and Latinx populations.
The nexus between households’ indebtedness and consumption: the role of gender, geographical area and income groups
Authors: Wong Zun Yuan , Kusairi Suhal , Abdul Halim Zairihan 
Published: November 2022

Purpose
The persistent increase in household indebtedness is an alarming issue that is becoming a major concern for economists and governments in developing nations. Although household consumption is an essential source of economic growth, households’ failure to meet their financial obligations will be one of the causes of economic problems if the increase in consumption is largely financed by household borrowing. Therefore, this study aims to analyse the nexus between households’ indebtedness and consumption and the roles of household characteristics.

Design/methodology/approach
This study uses a microdata set of the Household Expenditure and Income Survey in 2019, which contained a simple random sampling of 4,730 households.

Findings
Using a simultaneous equations model, our results show a negative nexus between households’ consumption and their indebtedness. We find that household savings and size have an indirect impact on the debt service ratio, while the assets and total debt repayment instalments indirectly influence household consumption. We also identify differences in the relationship between the gender of the household head, rural and urban locations and income groups in consumption and indebtedness.

Research limitations/implications
The implication of this study is that governments should adopt several programmes to increase the awareness of household financial and debt management, especially for those in the low-income group.

Originality/value
This study contributes to the empirical literature by establishing a microeconomic perspective of consumption and an indebtedness model focusing on the differences in household characteristics in explaining consumption and indebtedness.
Suicide in prisons: describing trends and staff knowledge and preparedness to address suicide
Authors: Freese Rebekah A. , Canada Kelli E. , Nichols Pagena M. , McNamara Brianna 
Published: November 2022

PurposeSuicide prevention and intervention in prisons is a challenge. Prisons were not designed to be clinical facilities, yet with the growing numbers of people who face mental health challenges in prisons, staff require knowledge and skills to adequately address mental health crises, especially suicide. This study aims to: describe trends in suicide attempts and completions within one state’s prison system and measure staff knowledge and preparedness to address suicide.Design/methodology/approachThis research uses a nonexperimental research design and two data sources. Administrative data from 2000 to 2017 on serious suicide attempts and completions were analyzed, and all correctional staff employed in the state’s Department of Corrections were surveyed at one point in time. Univariate and bivariate analyses were conducted.FindingsThe number of serious suicide attempts trended up but completed suicides decreased. Correctional staff demonstrated high suicide knowledge of risk factors and warning signs of suicide. Staff who viewed a media-based suicide training displayed significantly more knowledge of suicide and perceived greater preparedness compared to staff who did not or did not recall viewing the training.Originality/valueCorrections staff play a key role in preventing suicides in prison. Innovative intervention is needed to increase suicide awareness, improve communication and enhance prevention skills.
Returning for skill or popularity? The demand for esports match replay
Authors: Wang Wenche 
Published: November 2022

PurposeAmong scholarly analyses of sport demand, few have considered the demand for match replays despite replays' indispensable role in sport broadcasts. Different from live matches, viewers of match replays generally have access to match outcomes. Outcome uncertainty is thus less relevant in attracting viewers. This paper addresses this research gap by analyzing the demand for replays of competitive esports matches.Design/methodology/approachWe collected match replay data for two competitive esports leagues, CrossFire Pro League and King Pro League, from two Chinese esports online platforms, Douyu.com and Huya.com. Drawing on two economic theories of superstar effects—a skilled-based theory and a popularity-based theory, we performed regression analyses and dominance analyses to examine the demand for esports match replays.FindingsOur results indicated skill to be more important in the demand for tactical games while non-skill-popularity is more instrumental in attracting viewers to replays of leisurely games. The platform with knowledge-sharing features also exhibited a stronger skill impact for tactical games.Originality/valueFindings contribute to the understanding of sport match replays and offer implications for esports organizers and broadcasters to market games effectively.
Strategic international human resource management (SIHRM) framework: an integrated review and directions for future research
Authors: Patrick Warren Stanley , Jha Jatinder Kumar , Sharma Kumari Gargee 
Published: November 2022

PurposeThis study aims to review all frameworks of strategic international human resource management (SIHRM) published between 1990 and 2022 to ascertain their relevance in the current context with a focus on methodologies and theories in the post-pandemic era.Design/methodology/approachIn this study a pool of 69 papers published in 16 journals was considered for full-text evaluation using a set of relevant keywords and pre-defined inclusion/exclusion criteria.FindingsThe number of qualitative research papers is the highest (90%) and theoretical perspectives are dominated by research-based (17%), institutional (17%) and SIHRM (14%) theories that emphasize competitive advantage, resource dependence and multiple SIHRM frameworks.Research limitations/implicationsThis research incorporates dominant theoretical perspectives and methodologies within an integrated SIHRM framework which accommodates the post-pandemic era.Practical implicationsThe integrated SIHRM framework reinforces the alignment of multiple contexts, dimensions, models and proportions to enable effective decisions for mitigating the current crisis and future research.Originality/valueThis research integrated a hybrid model of SIHRM by aligning the relevant existing SIHRM frameworks, which management can choose from to leverage the benefits that distributed remote work in an international context and decide what is most suitable for their businesses as they prepare for the future.
To share or not to share screens with customers? Lessons from learning theories
Authors: Roten Yonathan Silvain , Vanheems Regine 
Published: November 2022

Purpose
Even as retailers add digital features to their physical stores and equip their service teams with digital devices, no research has addressed the implications of frontline employees (FLEs) sharing a screen side-by-side with customers as a contemporary service practice. This paper aims to identify the potential customer benefits of this service practice.

Design/methodology/approach
Noting the lack of theoretical considerations of screen-sharing in marketing, this paper adopts an interdisciplinary approach and combines learning theories with computer-supported collaborative learning topics to explore how screen-sharing service practices can lead to benefits or drawbacks.

Findings
The findings specify three main domains of perceived benefits and drawbacks (instrumental, social link, individual control) associated with using a screen-sharing service. These three dimensions in turn are associated with perceptions of accepted or unaccepted expertise status and relative competence.

Research limitations/implications
The interdisciplinary perspective applied to a complex new service interaction pattern produces a comprehensive framework that can be applied by services marketing literature.

Practical implications
This paper details tactics for developing appropriate training programmes for FLEs and sales teams. In omnichannel service environments, identifying and leveraging the key perceived benefits of screen-sharing can establish enviable competitive advantages for service teams.

Originality/value
By integrating findings of a qualitative research study with knowledge stemming from education sciences, this paper identifies some novel service postures (e.g. teacher, peer, facilitator) that can help maximise customer benefits.
Engaging in cybersecurity proactive behavior: awareness in COVID-19 age
Authors: Alsmadi Duha , Maqousi Ali , Abuhussein Tala 
Published: November 2022

PurposeDue to the lack of awareness and poor cybersecurity practices that pose cyber threats during COVID-19 time, this research aims to explore user's attitude toward engaging in proactive cybersecurity awareness behavior.Design/methodology/approachBased on the theory of planned behavior, the relationship between multiple factors and their influence on the attitude is explored. A survey-based approach was utilized to collect responses and a model was proposed and tested on 229 respondents from the University of Petra-Jordan.FindingsThe attitude was significantly influenced by peers' influence and the individuals' cybersecurity threats awareness, especially threats that emerged during the COVID-19 time.Research limitations/implicationsThe research benefits decision makers in educational institutions who intend to develop cybersecurity awareness programs and helps them to assess user cybersecurity background weaknesses.Originality/valueThe research is the first to explore users' knowledge dimensions including organizational, information systems and social media as well as peers' influence on cybersecurity awareness. Also, it sheds light on the users’ perception of major cybersecurity hazards in COVID-19 time.