bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristic clinicopathological findings, prognosis and Epstein–Barr virus infection in rheumatoid arthritis patients with methotrexate-associated T/NK-lymphoproliferative disorders
Authors: Kimura Shoichi , Oshiro Yumi , Iwasaki Hiromi , Kadowaki Masanori , Ogata Masao , Daa Tsutomu , Sakata Toshifumi , Kawauchi Shigeto , Wang Ziyao , Takamatsu Yasushi , Takeshita Morishige 
Published: May 2022

Abstract
Background: Methotrexate (MTX)-associated T/natural killer (NK)-cell lymphoproliferative disorders (TNKLPDs) are rare in patients with rheumatoid arthritis (RA), and patients generally recover after MTX withdrawal. Methods: We investigated the clinicopathological characteristics, genetic findings, therapeutic response, and prognostic factors in 21 RA patients with MTX-associated TNKLPDs, which were compared with those of 38 with MTX-associated B-cell LPDs (BLPDs). Results: Immunohistologically, 11 patients (52%) showed CD4+ TLPDs, and seven had a T follicular helper (TFH) phenotype. The other nine patients (43%) showed CD8+ TLPDs, and remaining one (5%) had features of CD3+ CD4− CD8− CD30+ nasal type TNK-cell lymphoma. CD30+, p53+, and CMYC+ atypical lymphocytes were identified in seven (33%), eight (38%), and five (24%) patients, respectively. In situ hybridisation detected Epstein–Barr virus (EBV)-encoded RNA (EBER)+ large atypical lymphocytes in five patients (24%). Nine of 17 patients (53%) showed clonal peaks of TCRγ by polymerase chain reaction. MTX withdrawal was effective in 12 patients (57%), and eight (38%) received chemotherapies. Two patients with TFH+ or EBV+ CD4+ CD30+ large cell peripheral T-cell lymphoma (PTCL), one of CD8+ systemic anaplastic large cell lymphoma, and two of systemic EBV+ CD8+ T-cell lymphoma showed a lethal progressive clinical course within 13 months. Over than 500 U/L LDH, large atypical lymphocytes, expressions of CD30, p53, CMYC, and EBER in atypical lymphocytes were significantly poor prognostic factors for overall survival (p < 0.05, respectively). Mean interval from RA onset to TNKLPDs was 72 months, which was shorter than 166 in BLPDs (p = 0.003). EBV+ atypical and reactive lymphocytes were frequently found in 15 patients with TNKLPDs (71%) and 27 with BLPDs (71%). Conclusions: RA patients with MTX-associated TNKLPDs occurred in early phase of RA, compared with those of the BLPDs, and occasionally showed a lethal progressive clinical course. Detection of MTX-associated TNKLPD patients with poor prognostic factors is necessary. EBV infection in the immunosuppressive states due to persistent RA, MTX and other biological drugs may play a role in forming the tumour microenvironment and lymphomagenesis of TNKLPDs.
Determination of Ki67 after SARS-CoV-2 infection by enzyme linked immunoassay
Authors: Kldiashvili Ekaterina , Iordanishvili Saba , Shotadze Tamar , Lomidze Mariam 
Published: May 2022

Abstract
Objectives
The immunity status after the sever acute respiratory syndrome coronavirus-2 (SARS-CoV-2) can determine the protection against reinfection and efficacy of vaccination. Although the mechanisms of SARS-CoV-2 induced immune response need further investigation to provide more data on innate and adaptive immune responses. It can be expected that in case of fail of SARS-CoV-2 induced humoral immunity the adaptive one is developed. The NovaLisa SARS-CoV-2 (COVID-19) IgG ELISA diagnostical kit (NovaTec Immunodiagnostica GmbH, Germany) for in vitro diagnostic and Human Ki67 ELISA kit (MyBioSource Inc, USA) were used. The study has been performed according to the ELISA kits specific protocols; the absorbance was measured at 450 nm wavelength. For the calculation of results we used the manufacturers’ guidelines.
Results
It has been revealed that: a) 16 (32.7%) samples are negative; b) 5 (10.2%) samples are equivocal; and c) 28 (57.1%) samples are positive for IgG for nucleocapsid protein of SARS-CoV-2. Median levels of Ki67 in: a) negative for IgG of SARS-CoV-2 nucleocapsid protein samples is 14.95 ng/ml; b) equivocal samples is 10.6 ng/ml; and c) positive - 8.3 ng/ml. Increased level of Ki67 correlates with the negative status for IgG of SARS-CoV-2 nucleocapsid protein.
Combination of long-term 13CO2 labeling and isotopolog profiling allows turnover analysis of photosynthetic pigments in Arabidopsis leaves
Authors: Banh Anh Thi-Mai , Thiele Björn , Chlubek Antonia , Hombach Thomas , Kleist Einhard , Matsubara Shizue 
Published: May 2022

Abstract
Background: Living cells maintain and adjust structural and functional integrity by continual synthesis and degradation of metabolites and macromolecules. The maintenance and adjustment of thylakoid membrane involve turnover of photosynthetic pigments along with subunits of protein complexes. Quantifying their turnover is essential to understand the mechanisms of homeostasis and long-term acclimation of photosynthetic apparatus. Here we report methods combining whole-plant long-term 13CO2 labeling and LC-MS analysis to determine the size of non-labeled population (NLP) of carotenoids and chlorophylls (Chl) in leaf pigment extracts of partially 13C-labeled plants.Results: The labeling chamber enabled parallel 13CO2 labeling of up to 15 plants of Arabidopsis thaliana with real-time environmental monitoring ([CO2], light intensity, temperature, relative air humidity and pressure) and recording during the experiment. No significant difference in growth or photosynthetic pigment composition was found in leaves after 7-d exposure to normal CO2 (~400 ppm) or 13CO2 in the labeling chamber, or in ambient air outside the labeling chamber (control). Following chromatographic separation of the pigments and mass peak assignment by high-resolution Fourier-transform ion cyclotron resonance mass spectrometry (MS), mass spectra of photosynthetic pigments were analyzed by triple quadrupole MS to calculate NLP. The size of NLP remaining after the 7-d 13CO2 labeling was ~10.3% and ~11.5% for all-trans- and 9-cis-β-carotene, ~21.9% for lutein, ~18.8% for Chl a and 33.6% for Chl b, highlighting non-uniform turnover of these pigments in thylakoids. Comparable results were obtained in all replicate plants of the 13CO2 labeling experiment except for three that were showing anthocyanin accumulation and growth impairment due to insufficient water supply (leading to stomatal closure and less 13C incorporation).Conclusions: Our methods allow 13CO2 labeling and estimation of NLP for photosynthetic pigments with high reproducibility. The results indicate distinct turnover rates of carotenoids and Chls in thylakoid membrane, which can be investigated in the future by time course experiments. Since 13C enrichment can be measured in a range of compounds, long-term 13CO2 labeling chamber, in combination with appropriate MS methods, facilitates turnover analysis of various metabolites and macromolecules in plants on a time scale of hours to days.
Prevalence of Cryptosporidium infection in Cattle in the Federal Capital Territory, Nigeria: Associated drivers and public health challenge
Authors: adeiza abdulrahman Musa , Alhaji Nma Bida , Godwin Enid , Nafarnda Wesley Daniel , Omeiza Gabriel Kehinde , Chinwe Elizabeth 
Published: May 2022

Abstract
This study was undertaken to detect and identify the oocysts of Cryptosporidium and determine its prevalence using cattle feces in the FCT, Nigeria. A total of 400 Cattle fecal samples were screened for Cryptosporidium oocysts using Safranin-Methylene Blue (SMB) staining technique. Cryptosporidium Oocysts were detected in 17.8% (71/400) of the samples. Prevalence of Cryptosporidium in cattle was highest (32.4%, 22/68) in the Gwagwalada Area Council. There was a significant difference in the prevalence of Cryptosporidium and Area Councils of the sampled cattle in the FCT (X2 = 16.55; p = 0.005). The questionnaire surveyed variables include cattle breed, age, sex, source of cattle drinking water, consistency of feces, size of herd, and herd management practices. Age, consistency of feces, breed, management practices and size of herd were all associated with prevalence of Cryptosporidium (P < 0.05) in the sampled cattle in FCT. Sex was not associated (P > 0.05) with prevalence of Cryptosporidium This study demonstrated a widespread infection of cattle with Cryptosporidium in the FCT. It is hoped that the outcome of this study would facilitate laboratories to make timely communication of the parasitic infection to the public health authorities for effective control measures.
Design Proposal for High-Sensitive Infrared Metamaterial-Based Sensors
Authors: Askari Mehdi , Farrokhi Zahra , Pakarzadeh Hassan 
Published: May 2022

Abstract
Metamaterial-based infrared sensors have attracted a lot of attention due to their ease of fabrication and various applications. By studying the performance of metamaterial sensors, it has been shown that their sensitivity has the potential of being considerably enhanced. In this paper, sensing capability of various metamaterial architectures, with different geometrical designs, is investigated. The unit cell of the initial considered metamaterial architecture contains two layers so that the first layer consists of two metallic nano-bars and two nano-plates, while the second layer includes a uniform silver plate. For this structure, a nearly perfect absorption behavior, along with a sensing capability with the sensitivity value of 2720 nm/RIU, is observed. By applying some changes to the geometry of this structure, it is found that, for some of the new designs, not only the original behavior of perfect absorption is replaced by the fascinating behavior of electromagnetically induced reflectance (EIR), but also the sensitivity of the corresponding sensors is considerably raised as well. The most interesting result is achieved when the uniform silver plate is completely removed, two cut-bars are horizontally emptied out of the two nano-plates, and a π-shaped metallic element is inserted between the two nano-plates. For this design, an EIR behavior, as well as a high sensitivity value of 4077.2 nm/RIU, is observed. Besides, effects of geometrical parameters on the sensitivity and resonance frequency of the sensors are investigated. It is found that the resonance frequency can be tuned in the range of 250–350 THz. This tunability of the resonance frequency is promising for practical applications.
Orthostatic Intolerance as a Potential Contributor to Prolonged Fatigue and Inconsistent Performance in Elite Swimmers
Authors: Petracek Lindsay S. , Eastin Ella F. , Rowe Ian R. , Rowe Peter C 
Published: May 2022

Abstract
BackgroundAthletic underperformance is characterized by fatigue and an inability to sustain a consistent exercise workload. We describe five elite swimmers with prolonged fatigue and athletic underperformance in whom an approach that focused on orthostatic intolerance was associated with improved function.MethodsParticipants were referred for evaluation of fatigue and underperformance to the Chronic Fatigue Clinic at the Johns Hopkins Children’s Center. All patients were evaluated for overtraining syndrome, as well as for features commonly seen in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). The latter included joint hypermobility, orthostatic intolerance, and non-IgE mediated milk protein intolerance. Orthostatic intolerance was tested by performing a ten-minute passive standing test or a head-up tilt table test. ResultsOrthostatic testing provoked fatigue and other symptoms in all five swimmers, two of whom met heart rate criteria for postural tachycardia syndrome. Treatment was individualized, primarily consisting of an increased intake of sodium chloride and fluids to address orthostasis. All patients experienced a relatively prompt improvement in fatigue and other orthostatic symptoms and were able to either return to their expected level of performance or improve their practice consistency. ConclusionsOrthostatic intolerance was an easily measured and treatable contributor to athletic underperformance in the five elite swimmers we describe. We suggest that passive standing tests or formal tilt table tests be incorporated into the clinical evaluation of athletes with fatigue and underperformance as well as into scientific studies of this topic. Recognition and treatment of orthostatic intolerance provides a new avenue for improving outcomes in underperforming athletes.
Effects of aqueous extract of red propolis as an alternative to synthetic antimicrobial for quails enhanced egg quality and maintained health status
Authors: Garcia Patricia López , Stefani Lenita , Boiago Marcel , Araujo Denise , França Arieli , Sponchiado Bruno , Nesi Daniela , Deolindo Guilherme , Rampazzo Maiara , Migliorini Marcos , Junior Paulo de Oliveira 
Published: May 2022

Abstract
This study aimed to determine the effects of aqueous extract of red propolis (RP) as an alternative to synthetic antimicrobial for quails enhanced egg quality and maintained health status. A total of 120 laying quails with 52 days of age were were randomly allotted to dietary treatments and six replicates of five birds each in completely randomized design. There were four dietary treatments as following: Control (TC), with enramycin (TE), 1g RP/kg of feed (RP1), 2g RP/kg of feed (RP2) in concetrated diet raised for 63 days. The results of productive performance such as percentage of egg production, feed intake per quail per day, average egg weight, egg mass, foods conversions showed no significant difference between treatments. The quails fed with red propolis in fresh eggs showed darker yolk (P < 0.05), with higher intensity of red and lower intensity of yellow; as less than 6.0 pH yolk; microbiological characteristics analyzed on the surface of eggs and feces of quails was lower and less than compared with other treatments. It is possible to conclude that red propolis showed promising results to be used as a feed additive for laying quails, since it maintained the productive performance of these animals and caused qualitative improvements in the physicochemical and microbiological characteristics of the eggs.
Morphological evolution of recycled Inconel 718 powder for LPBF process
Authors: Ruiz-Huerta Leopoldo , Moock Verena Margitta , Ulloa-castillo Nicolás A , Segura-Cárdenas Emmanuel , Vázquez Alan Borbolla , Elias-Zuniga Alex , Caballero-Ruiz Alberto 
Published: May 2022

Abstract
Metallic powder alloys for Additive Manufacturing (AM) are required to have a certain particle size and shape distribution in different processes such as metal powder bed fusion. For reasons of sustainability and cost effectiveness, it is common to recycle powder that was not melted in the build construction process. The influence of the particle size and shape distribution of the feedstock material has an impact on the AM process, but also on the structural properties of the final built part. As usual in a Laser Powder Bed Fusion (LPBF) process, the metal powder undergoes a heuristically designed sieving and blending procedure to maintain a certain particle distribution. However, keeping track of the powder’s age during each building cycle can be obscure or laborious. To overcome this challenge, the authors of this research propose a theoretical model and an experimental methodology to study the powder morphology evolution with respect to a specific number of building cycles. Such morphological investigation was carried out through a scanning electron microscope, analyzing the morphological evolution of nickel-chromium alloy metal (Inconel 718) powder. The powder analysis gives a close fit of its mean age to the span of the retained particle solidity measurements, as well as the evolution of the mean major diameter. The findings from this methodology give an insight on the powder aging rate, which contributes to determining the powder’s effective lifetime, impacting positively on the organization and storage of metal powders for AM.
Americans’ Support for Adaptation Policies on Natural Disasters of Wildfires and Floods -Surprise After Surprise
Authors: MacInnis Bo , Krosnick Jon 
Published: May 2022

Abstract
According to many natural scientists, global warming has worsened wildfires and floods in the United States, causing increasingly devastating damage, and will do so in the future. Whether government takes action to better mitigate the risks and costs of these natural disasters may depend on public opinion. This study explored Americans’ preferences for policies to prevent and cope with such damage and the factors influencing these preferences. A telephone survey of a nationally representative sample of American adults in August 2020 showed that majorities, and sometimes huge majorities, favored a variety of specific policies to protect people from damage, most of which are not being implemented well currently. As expected, policy support was driven more by sociotropic reasoning than by self-interest but surprisingly was not smaller among racial minorities or lower income individuals, refuting Maslow’s hierarchical of need theory and the notion of a “finite pool of worry.” Challenging theories of issue framing and demonstrating the robustness of opinions on this issue, an experiment showed that policy support was not altered when increasing future damage from wildfires and floods were attributed to global warming. Americans overwhelmingly favored federal government involvement in prevention and coping but preferred that the costs be borne mostly by people in fire- and flood-prone areas. This evidence sends surprisingly strong signals to lawmakers about public preferences and highlights their contours and determinants, thus contributing to the development of theories of public opinion.
Construction of Chronic glomerulonephritis-related lncRNA-mRNA regulatory network and lncRNA-miRNA-mRNA ceRNA network by bioinformatics analysis
Authors: Zhuang Xingxing , Zhang Weifeng , Liu Tao , Wei Liangbing , Guo Yanyan , Gao Jiarong 
Published: May 2022

Abstract
BackgroundLong non-coding RNAs (lncRNAs), generally known as transcripts longer than 200nt, are important genetic regulators. Lately, researches on lncRNA-mRNA regulatory network and lncRNA-miRNA-mRNA competitive endogenous RNA (ceRNA) network has been the hotspots in the field of non-coding RNAs. The present study explored the functional roles and regulatory mechanisms of lncRNAs in chronic glomerulonephritis (CGN).MethodsIn the current study, the RNA-seq was performed to study the differences in the expression profiles of lncRNAs in lipopolysaccharide (LPS) -induced glomerular mesangial cells (GMCs). LncRNA-mRNA regulatory network and lncRNA-miRNA-mRNA competitive endogenous RNA (ceRNA) network were constructed to examine the role and mechanism of proliferation-related lncRNAs. To reveal the biological functions of lncRNAs, GO biological process and KEGG pathway analysis were we performed on all mRNAs involed in lncRNA-mRNA regulatory network and ceRNA network.Results1532 differentially expressed lncRNAs, including 594 upregulated lncRNAs and 938 downregulated lncRNAs were screened out by RNA-seq in LPS-induced GMCs. Furthermore, the lncRNAs-mRNAs regulatory network, including 236 lncRNAs and 556 mRNAs and lncRNA-miRNA-mRNA ceRNA network, including 6 lncRNAs, 18 miRNAs, and 419 mRNAs were successfully constructed. The GO biological process and KEGG pathway analysis demonstrated that lncRNAs were mainly related to inflammatory response and substance metabolism.ConclusionsThis study first identified key proliferation-related lncRNAs in LPS-induced GMCs, and further revealed a global view of lncRNAs-mRNAs regulatory network and lncRNA-miRNA-mRNA ceRNA network involved in CGN. Our findings offered novel insights into the roles of lncRNAs in the pathogenesis of CGN and provided promising diagnostic biomarkers.
Optically-triggered strain-driven Néel vector manipulation in a metallic antiferromagnet
Authors: Grigorev Vladimir , Filianina Mariia , Lytvynenko Yaryna , Sobolev Sergei , Pokharel Amrit , Sapozhnik Alexey , Kleibert Armin , Bodnar Stanislav , Grigorev Petr , Skourski Yurii , Kläui Mathias , Elmers Hans-Joachim , Jourdan Martin , Demsar Jure 
Published: May 2022

Abstract
The absence of stray fields, their insensitivity to external magnetic fields, and ultrafast dynamics make antiferromagnets promising candidates for active elements in spintronic devices. Here, we demonstrate manipulation of the Néel vector in the metallic collinear antiferromagnet Mn2Au by combining strain and femtosecond laser excitation. Applying tensile strain along either of the two in-plane easy axes and locally exciting the sample by a train of femtosecond pulses, we align the Néel vector along the direction controlled by the applied strain. The dependence on the laser fluence and strain suggests the alignment is a result of optically-triggered depinning of 900 domain walls and their sliding in the direction of the free energy gradient, governed by the magneto-elastic coupling. The resulting, switchable, state is stable at room temperature and insensitive to magnetic fields. Such an approach may provide ways to realize robust high-density memory device with switching timescales in the picosecond range.
Evaluating a tele-asthma self-management intervention, Canadian Asthma Text Messaging Study (CANATEXTS), among adults with asthma: study protocol for a pragmatic randomized controlled trial
Authors: Poureslami Iraj M , Tregobov Noah , Bergeron Celine 
Published: May 2022

Abstract
Background: While effective treatments are available, asthma control is suboptimal for many asthma patients. To help patients self-manage their asthma symptoms, provision of an asthma action plan (AAP), with instructions on managing worsening asthma symptoms is recommended, as a standard of care; however, only about half of patients correctly adhere to their AAP. Canadian Asthma Text Messaging (CANATEXTS) is a TH intervention facilitating access to an electronic AAP (eAAP) via a mobile device. In a feasibility study to assess the safety and efficacy of CANATEXTS, an 18% reduction in the relative risk of exacerbation was observed among the intervention group compared to control group. This study will assess the effectiveness of CANATEXTS on asthma outcomes in a Canada-wide study. Methods: This is a superiority a 2-arm, multisite randomized control trial (RCT). This study aims to determine if CANATEXTS reduces asthma exacerbations over a 12-month period, improves asthma control, QoL and medication adherence, and is cost-effective. The intervention includes access to an electronic asthma action plan (eAAP) on patients’ mobile devices, disease-related education, and weekly reminders via an SMS interface. The study will include 600 adult participants with asthma recruited from 14 respiratory clinics across Canada. Participants will be randomly assigned to either Intervention (eAAP) or Control (wAAP) groups. All outcomes of interest will be assessed during three in-person assessments (baseline, 6-month, and 12-month) and two telephone follow-ups (3-month and 9-month). Data will be analyzed with a linear mixed-effects model across all time points. Discussion: TH has the potential to improve adult individuals with asthma's engagement in self-management practices. Our feasibility study showed TH could yield a reduction in asthma exacerbations. If the proposed TH intervention is found to be effective for asthma management in a nation-wide trial, it will generate evidence to support integration of TH in asthma self-management. This study will also provide important information on the cost-effectiveness of CANATEXTS. Trial registration: ClinicalTrials.gov identifier: NCT02017795; date of registration: 06 May 2018 (registered prospectively)