bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contribution to the drosophilid flies (Diptera: Drosophilidae) of Sakartvelo (Georgia) with the new records for the country
Authors: Murvanidze Maka , Deutsch Ferenc , Inasaridze Nino , Japoshvili George 
Published: June 2022

Abstract
Four genera and 12 species of drosophilid flies have been recorded during our survey provided in five regions of Georgia. Two genera: Amiota (Loew, 1862) and Gitona Meigen, 1830, and four species: A. subtusradiata Duda, Drosophila busckii Coquillett, D. kuntzei Duda, 1924 and G. distigma Meigen, 1830 are new records for fauna of Sakartvelo (Georgia).
Implementing a General Practitioner-to-General Physician eConsult service (eConsultant) in Australia
Authors: Job Jennifer , Nicholson Caroline , Calleja Zoe , Jackson Claire , Donald Maria 
Published: June 2022

Abstract
BackgroundIn response to lengthy wait times for specialist outpatient appointments, electronic consultation (eConsult) services have developed globally, providing asynchronous, secure and timely communication between general practitioner (GP) and specialist. This study aims to assess implementation of a Queensland eConsultant service in two Australian Primary Health Networks (Western Queensland and Brisbane South) to understand key barriers and enablers to adoption prior to broader implementation.MethodsOur theory-informed mixed-methods evaluation assessed implementation between July 2020 and March 2022. Adoption and implementation activities were prospectively recorded in bespoke tracking spreadsheets with implementation activities coded against the Expert Recommendations for Implementing Change (ERIC) strategies. Semi-structured interviews with GPs and stakeholders informed by the Consolidated Framework for Implementation Research (CFIR) were conducted to understand determinants of implementation. ResultsOf the 40 practices invited to take part in the eConsultant service, 20 (50%) enrolled. Of the 82 GPs who consented, 37 sent at least one Request for Advice (RFA) to the eConsultant with a total of 112 RFA sent. Implementation was predominantly guided by eight strategies. Qualitative interviews were conducted with 11 GPs and 4 stakeholders (12 from rural/remote regions, 11 females and two sole practitioners). Interviewees felt the eConsultant service supported outpatient appointment avoidance and provided efficient, timely access to specialist support for GPs and their patients. Barriers identified to using eConsultant related to digital infrastructure, competing priorities, and keeping the service ‘front of mind’. Key enablers identified were the relative advantage of eConsultant over other options, patient benefits and COVD-19 facilitating the use of digital technology.ConclusionsThis evaluation highlighted service enablers as well as user priorities for broader implementation. A focus on a well-integrated digital system and availability of a variety of eConsultant specialties are seen as key strategies to embedding the eConsultant option in GP advice processes in Australia.
Optimal internal reference genes for statin-based cancer research
Authors: Irie Nanami , Warita Katsuhiko , Zhou Yaxuan , Tashiro Jiro , Oltvai Zoltán N. , Warita Tomoko 
Published: June 2022

Abstract
Statins have recently been reported to have anticancer effects and can be used as anticancer agents via drug repositioning. In reverse transcription quantitative polymerase chain reaction (RT-qPCR), the internal reference gene itself must not be affected by any experimental conditions. Since statins are considered to have a wide range of effects on cells via inhibition of the mevalonate pathway, there is a high possibility that statins might vary the expression of internal reference genes, thereby misleading the obtained gene expression data. The present study evaluates the expression stability of the internal reference genes. Statin-sensitive cancer cells (lung cancer-derived HOP-92, prostate cancer-derived PC-3, and melanoma-derived SK-MEL-5 and MDA-MB-435) and statin-resistant cancer cells (lung cancer-derived NCI-H322M and prostate cancer-derived DU-145) were used for the analysis. Atorvastatin was administered at seven different concentrations (0–30 µM). RT-qPCR was performed for the 15 internal reference genes, and then five algorithms were used to evaluate the stability of internal reference genes. Both statin-sensitive and statin-resistant cancer cells showed that atorvastatin affected certain internal reference genes in a dose-dependent manner. The effect of statins on internal reference genes is dependent on the cancer cell type, suggesting that caution should be exercised when comparing expression between cells.
Core Outcome Measures for Clinical Effectiveness Trials of Nutritional and Metabolic Interventions in Critical Illness: An International Modified Delphi Consensus Study Evaluation (CONCISE)
Authors: Davies Thomas , Gassel Rob van , de Poll Marcel van , Gunst Jan , Casaer Michael , Christopher Kenneth , Preiser Jean Charles , Hill Aileen , Gundogan Kursat , Reintam-Blaser Annika , Rousseau Anne-Françoise , Hodgson Carol , Needham Dale , Castro Melina , Schaller Stefan , McClelland Thomas , Pilkington Joshua , Sevin Carla , Wischmeyer Paul , Lee Zheng Yii , Govil Deepak , Li Andrew , Chapple Lee-anne , Denehy Linda , Montejo-González Juan Carlos , Taylor Beth , Bear Danielle , Pearse Rupert , McNelly Angela , Prowle John , Puthucheary Zudin 
Published: June 2022

Abstract
Background: Clinical research on nutritional and metabolic interventions in critically ill patients is heterogenous regarding time points, outcomes and measurement instruments used, impeding intervention development and data syntheses, and ultimately worsening clinical outcomes. We aimed to identify and develop a set of core outcome domains and associated measurement instruments to include in all research in critically ill patients.Methods: An updated systematic review informed a two-stage modified Delphi consensus process (domains followed by instruments). Measurement instruments for domains considered “essential” were taken through the second stage of the Delphi and a subsequent consensus meeting.Results: 213 participants (41 patients/caregivers, 50 clinical researchers, 122 healthcare professionals) from 24 countries contributed. Consensus was reached on time points (30 and 90 days post randomisation). Three domains were considered “essential” at 30 days (Survival, Physical function and Infection) and five at 90 days (Survival, Physical function, Activities of daily living, Nutritional status and Muscle/nerve function). Core “essential” measurement instruments reached consensus for Survival and Activities of daily living, and “recommended” measurement instruments for Physical function, Nutritional status, and Muscle/nerve function. No consensus was reached for a measurement instrument for Infection. Four further domains met criteria for “recommended”, but not “essential”, to measure at 30 days post randomisation (Organ dysfunction, Muscle/nerve function, Nutritional status, and Wound healing) and three at 90 days (Frailty, Body composition and Organ dysfunction). Conclusion: The CONCISE core outcome set is an internationally agreed minimum set of outcomes for use at 30 and 90 days post randomisation, in nutritional and metabolic clinical research in critically ill adults.
Malaria Spatio-Temporal Patterns in Busia and Tororo Districts, Eastern Uganda
Authors: Chemutai Faith , Ondijo Calvince Ochieng , Kisakye Joseph , Kabbale Fredrick , Egeru Anthony 
Published: June 2022

Abstract
Malaria burden remains one of the major public health challenges in sub-Saharan Africa, Uganda inclusive. Uganda has the 3rd highest global disease cases estimated to be 225 million and the 8th highest level of deaths equivalent to 781,000 per year. Malaria remains a leading cause of morbidity and mortality in Uganda, accounting for 30-50% of outpatient visits at health facilities, 15-20% of all hospital admissions, and up to 20% of all hospital deaths with at least 27.2% of inpatient deaths among children under five years of age. Widely recommended Plasmodium vector control approaches include utilization of long-lasting insecticidal nets and indoor residual sprays which are insecticide-based. This study assessed a nine-year period malaria cases data (2012-2020) obtained from the health management database to depict malaria spatial and temporal patterns in Busia and Tororo districts pre and post-vector control interventions. The routine malaria surveillance data reported passively through public and high-volume private health facilities were entered and manipulated into MS Excel. This was done separately for each of the 9 years. Considering the malaria cases registered on annual basis, Mann- Kendal test revealed a drastic decline of malaria cases over the nine-year period (2012 -2020) in Tororo district with Sen’s slope of -22, while for Busia district it revealed an increase of malaria cases (Sen’s slope +28). Equally, these trends reveal varied spatial patterns over the two districts. Both Busia and Tororo revealed a high prevalence of malaria between May-November in respect to bimodal rainfall pattern, matching with the perennial transmission setting of Uganda. This study has shown that, for further epidemiological characterization, vector behavior, biology and physiology need consistent monitoring and surveillance while implementing new vector control interventions targeting outdoor biting Plasmodium vectors.
Impact of Hematopoietic Cell Transplantation on Cardiovascular Risk Factors and Insulin Resistance
Authors: Ketterl Tyler , Chow Eric , Baker K. Scott , Ballard Sheri , Goodman Pam , Moran Antoinette , Steinberger Juliia , Leisenring Wendy , Dengel Donald , Sinaiko Alan , Koves Ildiko 
Published: June 2022

Abstract
Cardiovascular Disease (CVD) contributes to the increased risk of non-relapse late mortality after hematopoietic cell transplant (HCT). HCT survivors have a higher risk for insulin resistance and adverse CVD risk factors but the impact of exposure to HCT preparative conditioning regimens has not been clearly delineated. We enrolled 151 HCT recipients (26.4 ±0.9 years; time since HCT of 2.6-31.5 years) and 92 sibling controls to complete a cardiovascular risk assessment including insulin sensitivity by hyperinsulinemic euglycemic clamp, anthropometry, body composition by dual X-ray absorptiometry, blood pressure, and serum biomarkers. We used linear models to test for mean differences in all continuous outcomes between survivors and siblings, accounting for intra-family correlations with generalized estimating equations. Recipients of HCT were found to have lower insulin sensitivity and more likely to have adverse CVD risk factors in comparison to their healthy siblings. Significantly higher percent fat mass and visceral adipose tissue, and significantly lower lean body mass were noted in HCT recipients than sibling controls despite having a similar body mass index between the two groups. Total body irradiation in the conditioning regimen was one of the strongest factors associated with lower insulin sensitivity, dyslipidemia and abnormal body composition leading to sarcopenic obesity.
Aetiology and outcome of paediatric acute liver failure in Asian children
Authors: Ng Ruey Terng , Chew Kee Seang , Choong Chee Liang , Song Zhi Liang , Teh Jane Kimm Lii , Koay Zhong Ling , Wong Shin Yee , Kam Choy Chen , Ranai Norashikin Mohd , Lee Way Seah 
Published: June 2022

Abstract
Objective: Outcome of paediatric acute liver failure (PALF) in countries with limited availability of LT is not well described. We evaluated the outcome and prognostic indicators of PALF in Malaysia where emergency LT for ALF is limited.Methods: In this retrospective review on children <18 years with PALF, we compared clinical and laboratory parameters between survival after supportive treatment and after LT or succumbed without LT. The predictive values of Liver Injury Unit (LIU; peak laboratory values for international normalized ratio [INR], ammonia, total bilirubin) and upon admission (aLIU) on outcome of PALF were evaluated using receiver operator characteristic (ROC) curves. Results: Of 77 children (39 males [51%]; median age 2.8 years) with PALF, the overall survival was 55% (n=42); 52% (n=40) survived with supportive management, 2.6% (n=2) after LT. As compared to children who survived without LT, children who had LT/died had lower haemoglobin, aspartate transferase, g-glutamyl transpeptidase (GGT), and higher serum bilirubin, alkaline phosphatase, ammonia, and serum sodium (p <0.05). On multivariate analysis, significant independent predictor for death or LT were peak bilirubin >452 mmol/L and peak GGT <96 IU/L. The C-index of LIU and aLIU score were 0.79 and 0.68, respectively, indicating that LIU score was a good model in predicting outcome of PALF.Conclusions: Overall survival of PALF remained poor. High peak bilirubin and low GGT predict poor outcome of PALF. LIU score is a good model in predicting outcome of PALF and maybe useful in selecting children for emergency LT.
Evaluation of coal seam floor water bursting in multi aquifer situation: case study in the Gequan coal mine, China
Authors: Lv Situ , Zeng Yifan , Zhang Longqiang , Zhao Haonan 
Published: June 2022

Abstract
In order to ensure the safe production of Gequan coal mine, it is important to carry out a water bursting evaluation of the coal seam floor. This study selects vulnerability index method based on AHP as the evaluation method. Based on the analysis of the geological data in study area, water pressure, measured specific yield, equivalent thickness of effective aquiclude, brittle rock thickness under mining pressure damage zone, distribution of faults, distribution of collapse column, distribution of endpoints and intersection of fault and fault-scale index are taken as the evaluation index. As there are two aquifers in the lower part of the coal seam that are a threat to mining, the author evaluated Benxi limestone aquifer and Ordovician limestone aquifer separately, the reliability of the results was verified by comparing the results obtained with the water bursting point and some of the boreholes. The results of the evaluation provide guidance on the safe production of coal mines.
Cultivation practices can decrease genetic diversity in plant reintroduction programs
Authors: Diaz-Martin Zoe , De Vitis Marcello , Havens Kayri , Kramer Andrea , Mackechnie Linda , Fant Jeremie 
Published: June 2022

Abstract
Agricultural production practices can decrease genetic diversity in managed plant species meant for conservation programs. In this study we exposed seeds of three Viola species to different dormancy breaking conditions and show how prioritizing rapid germination likely imposes artificial selection, leading to selective sweeps and a genetic bottleneck that limits genetic diversity in all species. The ongoing use of typical horticultural production practices to propagate plant material may jeopardize the adaptive potential and long-term persistence of reintroduced populations.
Comparison of short-term and medium-term outcomes between intracorporeal anastomosis and extracorporeal anastomosis for laparoscopic left hemicolectomy
Authors: Wang Li-Ming , Jong Bor-Kang , Liao Chun-Kai , Kou Ya-Ting , Chern Yih-Jong , Hsu Yu-Jen , Hsieh Pao-Shiu , Tsai Wen-Sy , You Jeng-Fu 
Published: June 2022

Abstract
Background:Few studies have investigated the feasibility and safety of intracorporeal anastomosis (IA) for left hemicolectomy. This study aimed to investigate the potential advantages and disadvantages of laparoscopic left hemicolectomy with IA and compare the short- and medium-term outcomes between IA and extracorporeal anastomosis (EA).Methods:We retrospectively analyzed 133 consecutive patients who underwent laparoscopic left hemicolectomies from July 2016 to September 2019 and categorized them into the IA and EA groups. Short- and medium-term outcomes were compared.Results:The IA group had a shorter hospital stay, a shorter time to tolerate a liquid or soft diet, and higher serum C-reactive protein levels on postoperative day 3. There was no difference between both groups in operative time, postoperative pain, specimen length, or nearest margin. In addition, there were comparable 2-year overall survival (95.0% and 93.5%, p=0.747) and disease-free survival (97.5% and 90.9%, p=0.182) between both groups. Conclusions:Laparoscopic left colectomy with IA is technically feasible, with better short-term outcomes, including shorter hospital stays and faster tolerance to a liquid or soft diet. Higher postoperative serum C-reactive protein levels were observed in the IA group; however, no complications were observed. The medium-term outcomes were comparable to the overall survival and disease-free survival between IA and EA procedures.
Topographic ruggedness indices in ecology: past, present and future
Authors: Dilts Thomas E. , Blum Marcus E. , Shoemaker Kevin T. , Weisberg Peter J. , Stewart Kelley M. 
Published: June 2022

Abstract
Context:
Topographic ruggedness has been examined in thousands of ecological studies and is a popular variable for characterizing habitat selection. Despite widespread adoption, ruggedness metrics are often applied uncritically and require systematic and thorough testing using both artificial landscapes and real-world applications.
Objectives
In this paper we introduce a technique that removes the correlation of topographic ruggedness with curvature in order to more accurately represent fine-scale surface ruggedness.
Methods
We test our modified version of several ruggedness metrics against traditional ruggedness metrics using three ideal ruggedness criteria in artificial landscapes. We further tested our modified ruggedness measures using 449 real mountain ranges in Nevada, USA. Using desert bighorn sheep as a case study, we tested both modified and uncorrected ruggedness measures and slope in a multiscale context in order to examine habitat selection by female bighorn sheep.
Results
The modified versions of the metrics passed all three criteria of the ideal ruggedness test and was able to accurately capture surface ruggedness. Modified versions of ruggedness differed from uncorrected versions by containing fewer highly rugged cells along ridgelines and drainages. Habitat relationships of desert bighorn sheep with ruggedness were scale-dependent, such that female sheep selected for steep slopes at fine spatial scales and ruggedness at moderate spatial scales.
Conclusions
We demonstrate that there are three components to ruggedness: elevation variation, aspect diversity, and surface ruggedness representing first, second, and third generation ruggedness indices. Our technique for removing underlying topographic variation provides an improved mapping of surface ruggedness and augments the other two generations of ruggedness metrics.
Ocular Symptoms In COVID-19 Infection: A Survey Study
Authors: McHarg Matthew , Wang Yujuan , Yakin Mehmet , Zeleny Alex , Caplash Sonny , Sen Hatice Nida , Kodati Shilpa 
Published: June 2022

Abstract
Background: Coronavirus disease 2019 (COVID-19) systemic symptoms and sequelae have been studied extensively, but less is known about the characterization, duration, and long-term sequelae of ocular symptoms associated with COVID-19 infection. The purpose of this study was to analyze the frequency, spectrum, and duration of ocular symptoms in participants with COVID-19 infection treated in inpatient and outpatient settings.Methods: A retrospective electronic survey was distributed to NIH employees and the public who reported testing positive for SARS-CoV-2. The anonymous survey collected information on demographics, past ocular history, systemic COVID-19 symptoms, and ocular symptoms. Results: A total of 229 (21.9% male and 78.1% female, mean age 42.5 ±13.9) survey responses were included. Ocular symptoms were reported by 165 participants with a mean of 2.31±2.42 symptoms. The most commonly reported ocular symptoms were light sensitivity (31.0%), itchy eyes (24.9%), tearing (24.9%), eye redness (24.5%), and eye pain (24.5%). Participants with ocular symptoms had a higher number of systemic symptoms compared to participants without ocular symptoms (mean 9.17 ± 4.19 vs 6.22 ± 3.63; OR: 1.21; 95% CI: 1.11 – 1.32; p<0.001). Ocular symptoms were more common in those who reported a past ocular history compared to those who did not (81.8% vs 67.1%; OR: 2.17; 95% CI: 1.08 – 4.37; p=0.03). Additionally, the onset of ocular symptoms occurred most frequently at the same time as systemic symptoms (47.5%), and 12.6% reported symptoms lasting ≥14 days. Conclusions: Ocular surface-related symptoms are the most frequent ocular manifestations, and systemic disease severity is associated with the presence of ocular symptoms. Additionally, our results show that ocular symptoms can persist post-COVID-19 infection. Further work is needed to better understand ocular symptoms in COVID-19 and long-term sequelae.
A bioinformatics analysis: ZFHX4 promotes metastasis in ovarian cancer by modulating epithelial-mesenchymal transition and remodeling extracellular matrix
Authors: Zong Shuai , Xu Ping-ping , Xu Yin-hai , Guo Yi 
Published: June 2022

Abstract
Background
Ovarian cancer is a highly malignant gynecological tumor with high mortality worldwide. The poor prognosis mainly results from extensive metastasis at the time of presentation. Although some mechanisms of metastasis in ovarian cancer were proposed, few have been used in the clinical practice. In the study, we aimed to identify novel genes and pathways contributing to metastatic ovarian cancer from bioinformatics databases, thus providing more potential candidates for clinical applications.
Methods
Studies collecting matched primary tumors and metastatic lesions from ovarian cancer patients were searched in the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) between groups were screened by software R language. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for the DEGs were implemented by using online software Metascape. Venn diagram was plotted to present overlapping DEGs. The associations between the overlapping DEGs and prognosis were tested by Cox proportional hazard regression model using a cohort from the TCGA database. Genes affecting patients’ outcome significantly were served as hub genes. The mechanisms of the hub genes in promoting ovarian cancer metastasis were predicted using samples from TCGA database by R software.
Results
Two gene expression profiles (GSE30587 and GSE73168) met the inclusion criteria and were finally analyzed. A total of 362 genes were significantly differentially expressed in GSE30587, whereas 653 genes were in GSE73168. In GSE30587, DEGs were mainly involved in extracellular matrix (ECM) organization; For GSE73168, most of DEGs showed ion trans-membrane transport activity. Both of the DEG sets were related to system development. There were 8 overlapping genes between the two datasets (DCN, SVEP1, MS4A6A, FABP4, APBB1IP, VCAM1 and ZFHX4), and ZFHX4 was proved to be an independent adverse prognostic factor for ovarian cancer patients (HR = 1.40, 95%CI: 1.11–1.75, p = 0.004). Mechanistically, ZFHX4 was positively significantly correlated with epithelial-mesenchymal transition (EMT) markers (r = 0.54, p = 2.59×10− 29) and ECM-related genes (r = 0.52, p = 2.86×10− 27).
Conclusions
ZFHX4 could promote metastasis in ovarian cancer by regulating EMT and reprogramming matrisome, which rendered ZFHX4 as a potential therapeutic target and prognostic biomarker for ovarian cancer metastasis.