bluuebunny/crossref_metadata_2025_split huggingface dataset:
The cell wall of hornworts and liverworts: innovations in early land plant evolution?
Authors: Pfeifer Lukas , Mueller Kim-Kristine , Classen Birgit 
Published: April 2022

Abstract
An important step for plant diversification was the transition from freshwater to terrestrial habitats. The bryophytes and all vascular plants share a common ancestor that was probably the first to adapt to life on land. A polysaccharide-rich cell wall was necessary to cope with newly faced environmental conditions. Therefore, some pre-requisites for terrestrial life have to be shared in the lineages of modern bryophytes and vascular plants. This review focuses on hornwort and liverwort cell walls and aims to provide an overview on shared and divergent polysaccharide features between these two groups of bryophytes and vascular plants. Analytical, immunocytochemical, and bioinformatic data were analysed. The major classes of polysaccharides—cellulose, hemicelluloses, and pectins—seem to be present but have diversified structurally during evolution. Some polysaccharide groups show structural characteristics which separate hornworts from the other bryophytes or are too poorly studied in detail to be able to draw absolute conclusions. Hydroxyproline-rich glycoprotein backbones are found in hornworts and liverworts, and show differences in, for example, the occurrence of glycosylphosphatidylinositol (GPI)-anchored arabinogalactan-proteins, while glycosylation is practically unstudied. Overall, the data are an appeal to researchers in the field to gain more knowledge on cell wall structures in order to understand the changes with regard to bryophyte evolution.
Identification and characterization of the WYL BrxR protein and its gene as separable regulatory elements of a BREX phage restriction system
Authors: Luyten Yvette A , Hausman Deanna E , Young Juliana C , Doyle Lindsey A , Higashi Kerilyn M , Ubilla-Rodriguez Natalia C , Lambert Abigail R , Arroyo Corina S , Forsberg Kevin J , Morgan Richard D , Stoddard Barry L , Kaiser Brett K 
Published: April 2022

Abstract
Bacteriophage exclusion (‘BREX’) phage restriction systems are found in a wide range of bacteria. Various BREX systems encode unique combinations of proteins that usually include a site-specific methyltransferase; none appear to contain a nuclease. Here we describe the identification and characterization of a Type I BREX system from Acinetobacter and the effect of deleting each BREX ORF on growth, methylation, and restriction. We identified a previously uncharacterized gene in the BREX operon that is dispensable for methylation but involved in restriction. Biochemical and crystallographic analyses of this factor, which we term BrxR (‘BREX Regulator’), demonstrate that it forms a homodimer and specifically binds a DNA target site upstream of its transcription start site. Deletion of the BrxR gene causes cell toxicity, reduces restriction, and significantly increases the expression of BrxC. In contrast, the introduction of a premature stop codon into the BrxR gene, or a point mutation blocking its DNA binding ability, has little effect on restriction, implying that the BrxR coding sequence and BrxR protein play independent functional roles. We speculate that elements within the BrxR coding sequence are involved in cis regulation of anti-phage activity, while the BrxR protein itself plays an additional regulatory role, perhaps during horizontal transfer.
Unsupervised classification reveals consistency and degeneracy in neural network patterns of emotion
Authors: Doyle Cameron M , Lane Stephanie T , Brooks Jeffrey A , Wilkins Robin W , Gates Kathleen M , Lindquist Kristen A 
Published: April 2022

AbstractIn the present study, we used an unsupervised classification algorithm to reveal both consistency and degeneracy in neural network connectivity during anger and anxiety. Degeneracy refers to the ability of different biological pathways to produce the same outcomes. Previous research is suggestive of degeneracy in emotion, but little research has explicitly examined whether degenerate functional connectivity patterns exist for emotion categories such as anger and anxiety. Twenty-four subjects underwent functional magnetic resonance imaging (fMRI) while listening to unpleasant music and self-generating experiences of anger and anxiety. A data-driven model building algorithm with unsupervised classification (subgrouping Group Iterative Multiple Model Estimation) identified patterns of connectivity among 11 intrinsic networks that were associated with anger vs anxiety. As predicted, degenerate functional connectivity patterns existed within these overarching consistent patterns. Degenerate patterns were not attributable to differences in emotional experience or other individual-level factors. These findings are consistent with the constructionist account that emotions emerge from flexible functional neuronal assemblies and that emotion categories such as anger and anxiety each describe populations of highly variable instances.
Emerging ecophenotype: reward anticipation is linked to high-risk behaviours after sexual abuse
Authors: Pechtel Pia , Harris Jennifer , Karl Anke , Clunies-Ross Caroline , Bower Susie , Moberly Nicholas J , Pizzagalli Diego A , Watkins Edward R 
Published: April 2022

Abstract
Adolescents frequently engage in high-risk behaviours (HRB) following childhood sexual abuse (CSA). Aberrant reward processes are implicated in HRB, and their underlying fronto-striatal networks are vulnerable to neurodevelopmental changes during adversity representing a promising candidate for understanding links between CSA and HRB. We examined whether fronto-striatal responses during reward anticipation and feedback (i) are altered in depressed adolescents with CSA compared to depressed, non-abused peers and (ii) moderate the relationship between CSA and HRB irrespective of depression. Forty-eight female adolescents {14 with CSA and depression [CSA +  major depressive disorder (MDD)]; 17 with MDD but no CSA (MDD); 17 healthy, non-abused controls} completed a monetary reward task during functional magnetic resonance imaging. No differences in fronto-striatal response to reward emerged between CSA + MDD and MDD. Critically, high left nucleus accumbens activation during reward anticipation was associated with greater HRB in CSA + MDD compared to MDD and controls. Low left putamen activation during reward feedback was associated with the absence of HRB in CSA + MDD compared to MDD. Striatal reward responses appear to play a key role in HRB for adolescents with CSA irrespective of depression, providing initial support for a CSA ecophenotype. Such information is pivotal to identify at-risk youth and prevent HRB in adolescents after CSA.
The validation of the existence of the entero-mammary pathway and the assessment of the differences of the pathway between first and third parity sows
Authors: Greiner Laura L , Humphrey Dalton C , Holland Shayla N , Anderson C J , Schmitz-Esser Stephan 
Published: April 2022

Abstract
Forty sows (PIC Camborough 1050) from a single farm were randomly selected at 112 days of gestation to evaluate if gut bacteria transverse the blood system of the sow to deposit gut microbiota into the colostrum for piglet gut inoculation via the entero-mammary pathway. Fourteen first-parity gilts and 20 third-parity sows were used for the study. At the time of farrowing, colostrum, fecal samples, and blood samples were collected to evaluate the presence of bacteria in each sample. Colostrum and blood samples were processed via centrifugation to separate the immune cell fraction. Total DNA was extracted from fecal, colostrum, and white blood cell fractions. 16S ribosomal RNA gene amplicon sequencing was conducted at the Iowa State University DNA Facility (Ames, IA) to further characterize the bacterial and archaeal taxa present within each sample. Data were analyzed using Mothur and using R v4.0.3 (R Core Team, 2020). The experimental unit was the sow. Tables were generated to demonstrate the relative abundances of bacteria and archaea present in each type of sample and also identify organisms differentially abundant between sample types. Firmicutes were the most abundant phylum in colostrum and fecal samples and Tenericutes had the greatest abundance in blood comparative to other phyla. Further evaluation of the classification of bacteria present demonstrated that a few genera of bacteria are present in all three samples. Clostridum_sensu_stricto 1 was present in high relative abundance in colostrum and moderate abundance in the feces while also being present within the blood. Other genera present in all three sample types include Ruminococcus and Mycoplasma. In conclusion, the data suggest that there are bacteria present in all three locations of the sow at the time of farrowing and that first parity sows have different microbial populations than third parity sows.
Harboyan Syndrome: A Novel SLC4A11 Variant With Unique Genotype–Phenotype Correlation
Authors: Magan Tejal , Hammersmith Kristin M. , Viaene Angela N. , Kumar Priyanka , Eagle Ralph C. , Milman Tatyana 
Published: April 2022

Purpose:
The purpose of this study was to describe the genotypic and phenotypic characteristics of an infant with a SLC4A11 mutation associated with bilateral corneal edema, hearing loss, and hydronephrosis present since birth.

Methods:
This was a case report. Ophthalmic and systemic examination of the proband, histopathologic and ultrastructural characteristics of bilateral corneal discs, and molecular genetic evaluation by whole-exome sequencing are described.

Results:
A male infant was born with bilateral corneal opacities, sensorineural hearing loss, and hydronephrosis to healthy parents after an uneventful pregnancy. Penetrating keratoplasty of the left eye at age 10 months demonstrated minimal corneal edema with normal thickness Descemet membrane and cellular endothelium with intracytoplasmic vacuoles and degenerative changes in rare cells. Penetrating keratoplasty of the right eye 6 months later disclosed prominent corneal edema with a thickened posterior banded layer of Descemet membrane and severe endothelial atrophy. Whole-exome sequencing of the proband and parents' blood demonstrated a homozygous mutation in SLC4A11 gene (c.1735_1737delCTC,p.Leu579del). The combined clinical, histopathologic, and molecular genetic findings raised consideration of an unusual phenotype of Harboyan syndrome manifesting as congenital hereditary endothelial dystrophy with a prelingual rather than, as previously described, postlingual hearing loss.

Conclusions:
We report a novel homozygous SLC4A11 variant with a previously undocumented phenotype of CHED in association with prelingual sensorineural hearing loss and hydronephrosis, thus broadening our understanding of the spectrum of genotypic and phenotypic findings of Harboyan syndrome.
Case for Epithelium-Off Corneal Cross-linking
Authors: Dupps William J. 
Published: April 2022

Abstract:
The question of whether the epithelium should be removed in corneal cross-linking (CXL) in the treatment of keratoconus and other corneal ectatic disorders remains controversial. The motivation for epithelium-on CXL methods, which are not yet FDA approved and vary greatly in methodology, is to reduce the risk of vision-threatening complications related to debridement. However, as discussed in this counterpoint piece, most high-level evidence suggests that removal of the epithelium facilitates greater crosslinking effectiveness as measured by primary clinical outcome metrics such as topographic flattening and stabilization of disease. Furthermore, quality evidence is still lacking for a significant reduction in rates of infectious keratitis or loss of vision that can be attributed to debridement-related complications. In the absence of comparative effectiveness trials or long-term follow-up studies that show otherwise, the FDA-approved epi-off protocol is still the standard-bearer for safe and effective stabilization of corneal ectatic disease.
Colletotrichum asianum: A Novel Phytopathogen Causing Human Infection—A Case Report
Authors: Ghorpade Aditya , Anitha Venugopal , Ravindran Meenakshi 
Published: April 2022

Purpose:
The purpose of this study was to report the predisposing factors, clinical features, microbiological characteristics, and the management of the novel identified species, Colletotrichum asianum.

Methods:
Two patients, farmers by occupation, with a history of trauma with vegetative material, presented to us with redness and pain. The corneal scrapings revealed branched hyphae on potassium hydroxide mount, and woolly aerial fungal mycelia with gray colonies were seen on blood agar and potato dextrose agar. The findings were suggestive of a novel fungal organism C. asianum.

Results:
Both patients showed a remarkable response to commonly used antifungal agents. A healing response was obtained in 4 weeks. Molecular analysis by sequencing of the internal transcribed region of ribosomal DNA was performed to confirm the species C. asianum in both cases.

Conclusions:
To the best of our knowledge, this is the first case report describing infective keratitis secondary to C. asianum.
The relationship of frontal QRS-T angle between patients with newly diagnosed true and white coat hypertension
Authors: Akin Halil , Bilge Önder , Altintaş Bernas , Yildiz Görkem 
Published: April 2022

Objectives
The frontal QRS-T (f-QRST) angle is a measure of depolarization and repolarization heterogeneity and may be a predictor of poor ventricular health. We aimed to investigate whether the f-QRST angle indicates myocardial damage and predicts newly diagnosed true hypertension (HT) in patients with white coat hypertension.

Methods
We measured the f-QRST angle of 63 subjects with WHC and 105 patients with newly diagnosed HT. Laboratory tests and ABPM were followed up in all patients. The f-QRST angle was calculated on the surface ECGs.

Results
Of the patients in the study, 38.9% were female and 61.1% were male. The mean age was calculated as 59 ± 11 years. A comparison between both groups with the f-QRST angles was seen to be statistically significantly higher in the true HT group. The results of the receiving operating characteristic curve showed that the AUC value of the f-QRST angle was 0.94 (95% confidence interval, 0.91–0.97), the cutoff value was 60.5°, the sensitivity was 89.5%, and the specificity was 81%.

Conclusion
In our study, the f-QRST angle was found to be lower in patients with WHC than in true hypertensive patients. We think that ECG, which is a simple test, can be used to distinguish between true HT and WHC.
Androgen Deprivation and Sleep Disturbance
Authors: Myers Jamie S. , Siengsukon Catherine , Sherman Joseph , Shen Xinglei , Ptomey Lauren T. , Montgomery Robert , Bock Karen , Rice Anna , Parker William P. , Maliski Sally 
Published: April 2022

Background
Men receiving androgen deprivation therapy (ADT) for prostate cancer (PC) are at risk for cardiovascular comorbidities and cognitive changes. Interventional research involves in-person assessment of physical fitness/activity and cognitive function, which has been negatively affected by the COVID-19 pandemic. Androgen deprivation therapy–related hot flashes and nocturia increase risk for insomnia. Insomnia is associated with fatigue and may exacerbate ADT-related cognitive changes.

Objectives
The purpose of this mixed-methods pilot was to (1) determine feasibility/acceptability of remotely assessing physical fitness/activity, cognitive function, and sleep; (2) deliver telehealth cognitive behavioral training for insomnia (teleCBT-I) to improve sleep; and (3) garner qualitative feedback to refine remote procedures and teleCBT-I content.

Methods
Fifteen men with PC receiving ADT completed a 4-week teleCBT-I intervention. Videoconferencing was used to complete study assessments and deliver the weekly teleCBT-I intervention.

Results
Self-report of sleep quality improved (P < .001) as did hot flash frequency (P = .04) and bother (P = .025). Minimal clinically important differences were detected for changes in insomnia severity and sleep quality. All sleep logs indicated improvement in sleep efficiency. Remote assessment of fitness/cognitive function was demonstrated for 100% of participants. Sufficient actigraph wear time allowed physical activity/sleep assessment for 80%. Sleep actigraphy did not demonstrate significant changes.

Conclusions
Remote monitoring and teleCBT-I are feasible/acceptable to men with PC on ADT. Further research to confirm teleCBT-I efficacy is warranted in this population.

Implications for Practice
Preliminary efficacy for teleCBT-I interventions was demonstrated. Remote assessments of physical fitness/activity, sleep, and cognitive function may enhance clinical trial access for rural or economically disadvantaged PC survivors.
Barriers and Facilitators When Implementing Electronic Patient-Reported Outcome Measures at a Municipal Cancer Care Unit
Authors: Skåre Terese S. , Midtbust May Helen , Lund Jo-Åsmund , Kaasa Stein , Dreyer Anne 
Published: April 2022

Background
Few qualitative studies of barriers and facilitators when implementing electronic patient-reported outcome measure (ePROM) in municipal cancer care exist within the large body of symptom assessment research. Such data, gathered from healthcare professionals’ (HCPs) perspective, are central to the development and design of sustainable interventions aiming for a systematic and patient-centered symptom assessment to patients with cancer.

Objective
The aim of this study was to identify and explore barriers and facilitators, as described by HCPs, in the implementation of the ePROM application “Eir” at a municipal cancer care unit in Norway.

Methods
The study applies a qualitative method, conducting an inductive data inquiry of semistructured individual interviews and focus groups with 14 Norwegian HCPs. Analysis was inspired by thematic analysis as described by Braun and Clarke.

Results
The analysis revealed 3 main themes affecting the implementation of ePROM in municipal cancer care: “achieving patient-centered care,” “crucial management and training,” and “technological barriers.”

Conclusion
The results from this study suggest that HCPs’ motivation plays a significant role when implementing ePROM. Motivation of HCPs was strongly influenced by whether the application added value to previously used symptom assessment. Hands-on management and a multiprofessional approach enabled the implementation by facilitating adaptations, training, and resources.

Implications for Practice
The findings show that adapting the implementation of ePROMs to patient population could be of major importance. Early integration of ePROMs in cancer care could facilitate use throughout the disease trajectory.
Synergetic energy-conscious scheduling optimization of part feeding systems via a novel chaotic reference-guided policy
Authors: Zhou Binghai , Yi Qi , Li Xiujuan , Zhu Yutong 
Published: April 2022

PurposeThis paper aims to investigate a multi-objective electric vehicle’s (EV’s) synergetic scheduling problem in the automotive industry, where a synergetic delivery mechanism to coordinate multiple EVs is proposed to fulfill part feeding tasks.Design/methodology/approachA chaotic reference-guided multi-objective evolutionary algorithm based on self-adaptive local search (CRMSL) is constructed to deal with the problem. The proposed CRMSL benefits from the combination of reference vectors guided evolutionary algorithm (RVEA) and chaotic search. A novel directional rank sorting procedure and a self-adaptive energy-efficient local search strategy are then incorporated into the framework of the CRMSL to obtain satisfactory computational performance.FindingsThe involvement of the chaotic search and self-adaptive energy-efficient local search strategy contributes to obtaining a stronger global and local search capability. The computational results demonstrate that the CRMSL achieves better performance than the other two well-known benchmark algorithms in terms of four performance metrics, which is inspiring for future researches on energy-efficient co-scheduling topics in manufacturing industries.Originality/valueThis research fully considers the cooperation and coordination of handling devices to reduce energy consumption, and an improved multi-objective evolutionary algorithm is creatively applied to solve the proposed engineering problem.
COVID-19 and earnings management: a comparison between Chinese family and non-family enterprises
Authors: Rahman Md Jahidur , Ding Jinru , Hossain Md Moazzem , Khan Eijaz Ahmed 
Published: April 2022

PurposeThe main objective of this study is to examine the impact of the COVID-19 pandemic on earnings management practices in China using a sample of family and non-family enterprises. More specifically, this study aims to examine whether the COVID-19 pandemic causes variation in Chinese listed family and non-family enterprises' operations, as reflected in the level of real earnings management (REM).Design/methodology/approachThis study uses three standardised REM indicators, namely, the abnormal level of cash flows from operations, the abnormal level of production costs and the abnormal level of discretionary expenses. Ordinary least squares (OLS) regressions are applied to compare the earnings management of Chinese family and non-family enterprises during the pre-pandemic period (2017–2019) and the pandemic period (2020).Findings The authors find that Chinese listed non-family enterprises tend to participate in more REM activities than family enterprises before the COVID-19 outbreak. However, the opposite is true during the pandemic. The authors also find that COVID-19 has increased the involvement of family and non-family enterprises in REM activities.Originality/valueThe results of previous studies based on REM using Chinese listed firms may not be applicable under the new social background of COVID-19. As the period after the COVID-19 outbreak is relatively recent, Chinese researchers have yet to study it comprehensively. The present study is amongst the first empirical attempts investigating the effect of a pandemic financial reporting by investigating whether and how the burst of the COVID-19 crisis affected financial reporting through the earnings management practices of listed Chinese family and non-family enterprises. Such information is crucial because it can provide analysis for all stakeholders to make better decisions.