bluuebunny/crossref_metadata_2025_split huggingface dataset:
An 85-amino-acid polypeptide from Myrmeleon bore larvae (antlions) homologous to heat shock factor binding protein 1 with antiproliferative activity against MG-63 osteosarcoma cells in vitro
Authors: Ding Rui , He Ming , Huang Huoying , Chen Jing , Huang Mingxing , Su Yonghui 
Published: October 2022

Abstract

Background
Venomous arthropods have substances in their venom with antiproliferative potential for neoplastic cells.

Objectives
To identify a polypeptide from Myrmeleon bore (antlion) with antiproliferative activity against neoplastic cells, and to elucidate the molecular mechanism of the activity.

Methods
We used gel filtration and ion exchange chromatography to purify a polypeptide with antiproliferative activity against MG-63 human osteosarcoma cells from a proteinaceous extract of antlion. The polypeptide was sequenced and the stability of its antiproliferative activity was tested under a range of conditions in vitro. An 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the antiproliferative activity of the polypeptide against the MG-63 osteosarcoma cells and MC3T3-E1 mouse calvarial osteoblasts, which were used as a non-neoplastic control. We used western blotting to compare the levels of expression of heat shock transcription factor 1 (HSF1), heat shock protein 90 (HSP90), cyclin-dependent kinase 4 (CDK4), and protein kinase B alpha (ATK1) in MG-63 osteosarcoma cells and their mouse homologs in MC3T3-E1 osteoblasts after their treatment with the antlion antiproliferative polypeptide (ALAPP).

Results
The 85-amino-acid ALAPP has a 56% sequence identity with the human heat shock factor binding protein 1 (HSBP1). The antiproliferative activity of the polypeptide is relatively insensitive to temperature, pH, and metal ions. ALAPP has a strong concentration-dependent antiproliferative activity against MG-63 osteosarcoma cells compared with its effect on MC3T3-E1 osteoblasts. ALAPP significantly upregulates the expression of HSF1 in MC3T3-EL osteoblasts, but not in MG-63 osteosarcoma. ALAPP significantly downregulated the expression of HSP90, CDK4, and AKT1 expression in MG-63 osteosarcoma, but not in the osteoblasts.

Conclusions
ALAPP has significant antiproliferative activity against MG-63 osteosarcoma cells, but not nonneoplastic MC3T3-E1 osteoblasts. We speculate that non-neoplastic cells may evade the antiproliferative effect of ALAPP by upregulating HSF1 to maintain their HSP90, CDK4, and AKT1 expression at a relatively constant level.
Therapeutic alliance impact on analgesic outcomes in a real-world clinical setting: An observational study
Authors: Barrachina Jordi , Margarit César , Andreu Blanca , Zandonai Thomas , Ballester Pura , Muriel Javier , Cutillas Esperanza , Peiró Ana M. 
Published: October 2022

Abstract
A good therapeutic alliance is relevant for healthcare providers exposed to patients’ suffering, especially since patients and physicians may understand the painful experience differently. Our aim was to explore the impact of therapeutic alliance on analgesic outcomes in a real-world interdisciplinary pain unit (PU). A cross-sectional observational study was conducted on outpatients (n = 69) using opioids on a long-term basis for the treatment of chronic non-cancer pain, where clinical pharmacologists and pharmacists advised patients about their opioid treatment. Responses to the patient-doctor relationship questionnaire (PDRQ), sociodemographic and clinical information (pain level, quality of life and hospital use) were collected, whereas pharmacology data (analgesic prescription, adverse events, and compliance) were obtained from electronic health records. Patients were predominantly middle-aged (75 % women, 72 % retired), experiencing moderate pain (VAS 40–70 mm) on average, and under a high morphine equianalgesic dosage (95 ± 88 mg per day, mainly tapentadol or fentanyl). Patients with better PDRQ outcomes, and therefore better therapeutic alliance, showed lower pain intensity than patients with worse PDRQ outcomes (pain intensity: high scores 60 ± 47 mm and medium scores 60 ± 45 mm vs. low scores 80 ± 75 mm, p < 0.01). Along with this, pain intensity was lower when patients affirmed that, thanks to the health-care providers, they “gained new insight”, “felt better”, or “felt content with their doctor’s treatment”. What´s more, patients who affirmed “I benefit from the treatment” experienced increased pain relief (benefit 40 ± 30 vs. non-benefit 19 ± 26 mm, p = 0.010) and improved quality of life (benefit 33 ± 25 vs. non-benefit 18 ± 16 mm, p = 0.031). However, there was a percentage of patients who did not fully understand the provided information, which is something to be taken into account to improve in clinical routine. Therapeutic alliance supported by pharmacist experts on pain management can be an effective strategy to improve analgesic outcomes. Further efforts are needed to improve communication strategies for pain management. Future directions of research should include the analysis of the role of the pharmacist in poly-professional consultations as related to the advice of patients about their medication, and the mutual trust with the patients.
Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway
Authors: Shu Chen , Yan-Yan Zhang , Hai Zhang , Long-Kun Ding , Yue Xi , Man Yan , Chang Sun , Liang Wu , Hao Hu 
Published: October 2022

Abstract
This study evaluated the anti-inflammation effect of the three main short-chain fatty acids (SCFAs) on Acinetobacter baumannii-induced THP-1 cells. The three main SCFAs could inhibit A. baumannii-stimulated THP-1 cell NF-κB pathway activity and the expressions of NLRP3 inflamma-some and GSDMD, and increase autophagy. The three main SCFAs, especially the sodium butyrate (NaB), had the effect of down-regulation of ROS and TLR-2 expression in THP-1 cells. NaB and sodium propionate (NaPc), but not sodium acetate (NaAc), dramatically suppressed IL-1β and IFN-γ expression. The results indicated that NaB and NaPc could significantly inhibit the inflammation of THP-1 cells induced by A. baumannii, and the inhibitory effect was in the order of NaB > NaPc > NaAC. NaB and NaPc may inhibit inflammation through TLR-2/NF-κB/ROS/NLRP3 signaling pathway.
Synergistic action between a synthetic cannabinoid compound and tramadol in neuropathic pain rats
Authors: Quiñonez-Bastidas Geovanna Nallely , Osuna-Martínez Ulises , Reda-Licea Ana Laura , López-Ortíz Manuel , Regla Ignacio , Navarrete Andrés 
Published: October 2022

Abstract
In the present study the interaction of cannabinoid, PhAR-DBH-Me [(R, Z)-18-((1S,4S)-5-methyl-2,5-diazabicyclo[2.2.1]heptan-2-yl)-18-oxooctadec-9-en-7-ylphenyl-acetate] and tramadol in two neuropathy models, as well as their possible toxic effects, was analyzed. The anti-allodynic effect of PhAR-DBH-Me, tramadol, or their combination, were evaluated in neuropathic rats. Furthermore, the effective dose 35 (as the 35 % of the anti allodynic effect) was calculated from the maximum effect of each drug. Moreover, the isobolographic analysis was performed to determine the type of interaction between the drugs. A plasma acute toxicity study was carried out to assess the hepatic, renal, and heart functions after an individual or combined administration of the drugs, as well as histology using the hematoxylin-eosin or Masson-trichome method. PhAR-DBH-Me, tramadol, and their combination produced an antiallodynic effect on spinal nerve ligation (SNL) and cisplatin-induced neuropathic pain in rats. Moreover, PhAR-DBH-Me and tramadol combination showed a synergistic interaction in neuropathic pain rats induced by SNL but not for cisplatin-induced neuropathy. On the other hand, changes in renal and hepatic functions were not observed. Likewise, analysis of liver, kidney and heart histology showed no alterations compared with controls. Results show that the combination of PhAR-DBH-Me and tramadol attenuates the allodynia in SNL rats; the acute toxicology analysis suggests that this combination could be considered safe in administered doses.
Punicalagin attenuated allergic airway inflammation via regulating IL4/IL-4Rα/STAT6 and Notch- GATA3 pathways
Authors: Yu Li , Li Jianying 
Published: October 2022

Abstract
Allergic asthma is an inflammatory disease of the airways which has a complex etiology. Punicalagin, a major polyphenol present in pomegranates, is reported to possess various biological properties including antioxidant and antiproliferative effects. The current research aimed to evaluate the antiasthmatic effects of punicalagin in an ovalbumin (OVA)-induced experimental model of asthma in female BALB/c mice. Treatment group animals received punicalagin (12.5, 25 or 50 mg kg−1 body mass) per day for 21 days from day 1 of OVA injection. Dexamethasone (DEX) was administered to a separate group of mice, as the standard drug control. Inflammatory cell infiltration into the broncho-alveolar lavage fluid (BALF) was substantially decreased in punicalagin-treated mice. Punicalagin reduced Th2-derived cytokines and OVA-specific IgE levels. The IL-4/STAT6 and Notch/GATA3 signalling pathways were regulated on punicalagin administration. The data obtained illustrate the potency of punicalagin as an anti-asthmatic drug. Conclusively, the study’s observations suggest the potential therapeutic efficiency of punicalagin in allergic asthma.
Correlation analysis between Tervaert glomerular classification and clinical indicators in patients with type 2 diabetic nephropathy
Authors: Zhao Jing , Wu Yonggui 
Published: October 2022

Abstract

Purpose
To investigate the correlation analysis of Tervaert glomerular classification and clinical indicators in patients with type 2 diabetic nephropathy (DN).

Methods
We collected the renal sections of patients with DN and used immunoglobulin G (IgG), albumin (Alb), PAS, and MASSON staining to observe the extent of glomerular lesions. We simultaneously collected their clinical data for statistics and conducted correlation analysis with Tervaert glomerular classification. Moreover, we collected patients’ urine protein components data and conducted a correlation analysis with Tervaert classification.

Results
Tervaert classification reflects glomerular changes and was positively related to proteinuria, creatinine (Cr), and blood urea nitrogen but was negatively related to estimated glomerular filtration rate (eGFR). Tervaert classification was also positively related to total protein (TP), transferrin (TRF), retinol binding protein (RBP), N-acetyl-β-D aminoglucosidase (NAG), Alb, cystatin C (Cyc), IgG, β2-microglobulin (β2-MG), α1-microglobulin (α1-MG), Alb/Cr, and TP/Cr in urine.

Conclusion
Our study reveals that clinical indicators could well reflect glomerular lesions and has great value for the diagnosis and treatment of early DN.
The effects of global risk indicators on the MSCI emerging markets index
Authors: Öner Selma 
Published: October 2022

Abstract
The rising uncertainty in financial markets in the last 40 years has led to the creation of new financial indices that will enable these uncertainties to be defined and measured. For this purpose, the first volatility index created was the VIX Index as an indicator of uncertainty in the stock markets, which was followed by the OVX Index as an indicator of uncertainty in the oil markets and the GVZ Index as an indicator of uncertainty in the gold markets. These volatility indicesare also called “global risk indicators”. The MSCI (Morgan Stanley Capital International) Emerging Markets Index, which is the dependent variable of the study, is an index that is frequently followed by fund and portfolio managers in international markets and used as a benchmark. Therefore, in this study, the relationship between the MSCI EM Index and the global risk indicators for the period 28.03.2011-25.03.2022 was examined by the Toda-Yamamoto Causality Test. Afterwards, impulse-response and variance decomposition tests were applied to the variables. As a result of the study, causality relationships from global risk indicators to the MSCI EM Index were determined.
Economic and financial consequences of war in Ukraine: analysis of development scenarios
Authors: Bida Marianna , Ruda Iryna 
Published: October 2022

Abstract
The war affects the socio-economic state of Ukraine and is the cause of the deterioration of the economic situation of neighboring countries. Under such conditions, there is a need to carry out assessments of economic losses from the conduct of military operations and forecast the main economic indicators for the nearest period, taking into account the impact of the war. The purpose of the study is to calculate the losses to the economy, the destruction of residential facilities and critical infrastructure facilities in the first 100 days of the war, the assessment of the potential volume of the fall in GDP and the calculation of projected fiscal losses. The calculation of GDP reduction was based on the forecasts of the International Monetary Fund, the World Bank Group and the Government of Ukraine. The expected amount of GDP reduction is calculated using the GAP-analysis method, based on the forecasts made by these institutions in the prewar period, and the calculation of the level of GDP during the war. Based on the results of the conducted research, it was established that the economic losses from the war consist not only of the losses incurred and the reduction of GDP volumes, but also of the lost opportunities for development and unearned profits. In addition to the reduction in GDP, Ukraine’s losses are manifested in the increase in the level of public debt, the depreciation of the national currency, the reduction of gold and currency reserves, and the outflow of foreign direct investments3.
Exploring Lithuanian Real Estate Crowdfunding Investors’ Rationality
Authors: Legenzova Renata , Leckė Gintarė 
Published: October 2022

Abstract
The research aims to assess how rational investors are in making real estate crowdfunding investment decisions and weather similar rationality investors share common individual-level characteristics. The research data was collected through an online survey and investors’ rationality was assessed according to risk & return and behavioral biases criteria. Results revealed that 18% of the respondents were rational and as much as 78.1% of them demonstrated bounded rationality behavior. Further clustering of bounded rationality investors identified three distinct clusters and suggested that bounded rationality proved to be largely based on established behavioral biases rather than on failing to understand and apply risk and return criteria. In terms of individual-level characteristics, rationality of real estate crowdfunding investors significantly related to their financial knowledge, but not to their age, sex, income level, education, occupation, residence and interface with finance sector.
Dignity of Labour as the Bedrock of a Productive Society: Perspectives from the Nigerian Experience
Authors: Ogunola Abiodun Adekunle 
Published: October 2022

Abstract
The work that an individual engages in shapes and fulfils human dignity by providing for one’s need and that of his/her family as it allows an individual to contribute his/her own quota to the progress of a business venture, enterprise, or organization either personal or otherwise, and to appreciate the usefulness of such contributions. The respect for, protection of, and promotion of the dignity of the individual in the workplace should therefore be the ultimate aim that individual and organizations must strive for as this will help in creating Nigerian workplaces that are dignified, and when there are dignified workplaces, the society becomes more and more productive, as they continue to experience success, growth, progress, and development.
Employees as Brand Ambassadors: A Case Study Exploring the Impact of Employees’ Communication on Consumer Behavior
Authors: Šontaitė-Petkevičienė Miglė , Vaščėgaitė Aušrinė 
Published: October 2022

Abstract
The paper analyses the impact of employees’, as brand ambassadors’, communication on consumer behavior by empirically evaluating and comparing it from the view of customers. The theoretical part of the paper discusses the concept of brand ambassadors, specifics of employees as brand ambassadors and communication features related to it. After discussing theoretical insights on the topic, it is continued with the findings of the study from the selected Lithuanian case addressing main research question – how communication of employees as brand ambassadors affects consumer behavior. Research results proved that communication of employees as brand ambassadors increase customer interest and trust towards the company, however the impact towards purchase intention is not significant.
Decline of Losses in the Share of GDP Created by Russian Healthcare
Authors: Ragozin A. V. , Grishin V. V. , Glazunova S. A. , Shcheglova Yu. V. 
Published: October 2022

Introduction. Many developed countries have recognized the social sphere, including healthcare, as a full-fledged manufacturing sector of the economy, creating a significant share of value added in the sectoral structure of GDP. As part of the study, a comparison was made of the share of gross value added in the GDP of the United States, EU countries and Russia created by healthcare in 2018, as an example of the analysis of the impact of healthcare in different countries on gross value added, which is a significant indicator for assessing the correctness of the organization of healthcare financing.Material and methods. Used a comprehensive, statistical, comparative and retrospective analysis of available data.Research results. The data obtained indicate that the share of Russia's GDP created by healthcare is many times less than that of the United States and EU countries, which limits the growth of the Russian economy, causes poverty for a significant part of medical workers and hinders investment in the healthcare system and its demand for innovation.Discussion and conclusion. The authors see the root cause of the problems in the mistake of choosing the mandatory health insurance income system, the linkage of which to formal employment in a largescale shadow economy leads to the “free rider problem” (Free-Rider Problem) and a pronounced payment imbalance between providers and consumers of public health care due to its payment at deliberately unprofitable (below actual cost) tariffs. The most optimal solution is considered to be the replacement of salary-linked compulsory medical insurance contributions with a universal targeted tax for automated payment transactions.
PENGGUNAAN PROGRAM GEO-STUDIO SEEP/W UNTUK MENENTUKAN REMBESAN AIR LINDI PADA TANAH LEMPUNG
Authors: Aribudiman I Nyoman , Kurniawan Nggae Yohanes Erik 
Published: October 2022

Tanah merupakan bahan dan tempat konstruksi bangunan sipil berdiri yang terdiri dari beberapa unsur yang berperan penting bagi kehidupan manusia. Masalah yang sering dijumpai terkait tanah adalah pencemaran akibat air lindi yang dihasilkan oleh penumpukan sampah yang terlalu lama. Penelitian ini bertujuan untuk mengetahui rembesan air lindi pada tanah lempung dengan menggunakan program Geo-Studio Seep/W. Sampel limbah cair yang digunakan adalah air lindi dari TPA Suwung. Sampel tanah menggunakan tanah lempung yang diperoleh di Daerah Suwung, Denpasar, Bali. Pemodelan rembesan dengan Geo-Studio dilakukan pada kondisi tanah lempung yang dialiri oleh air murni (tanpa limbah) dan air lindi dari TPA Suwung. Pemodelan rembesan di Geo-Studio Seep/W dilakukan pada kondisi Saturated/Unsaturated. Hasil penelitian viskositas limbah cair menunjukkan bahwa air lindi TPA Suwung memiliki viskositas sebesar 0.002061309 N s/m2. Viskositas suatu fluida mempengaruhi nilai konduktivitas hidraulik tanah. Akibat pencampuran dengan air lindi, nilai konduktivitas hidraulik tanah juga mengalami penurunan. Nilai konduktivitas hidraulik tanah menurun dari 0.0001165 cm/s menjadi 0.0000328 cm/s untuk tanah lempung. Debit rembesan dan kecepatan rembesan terbesar pada tanah lempung melalui pemodelan di Geo-Studio Seep/W adalah akibat pengaliran air murni (tanpa limbah). Besarnya pengaruh perubahan debit rembesan atau kecepatan rembesan tanah lempung TPA Suwung akibat pengaliran air limbah lindi TPA Suwung terhadap debit rembesan atau kecepatan rembesan air murni sebesar 28,320%.
أستخراج المطر الفعال في محافظة نينوى
Authors: Khadr Mohammed Musa , Atiyah Khalid Satam 
Published: October 2022

يهتم البحث بإستخراج قيم المطر الفعّال في محافظة نينوى والعوامل المؤثرة فيه، والمتمثلة بخصائص عناصر المناخ و التبخر/النتح وعلاقته بتحديد قيم المطر الفعال ، تم إعتماد برنامج (CROP WAT) وإستخدام معادلة بنمان مونتيث الفاو 1998, على محطات منطقة الدراسة (الموصل، ربيعة، تلعفر، سنجار، تل عبطة ، البعاج) لإستخراج قيم المطر الفعّال وإستخراج قيم التبخر/النتح الممكن من كمية الأمطار والتي تشير إلى وجود عجز مائي خصوصاً خلال الخريف والربيع بسبب إرتفاع معدلات درجات الحرارة وزيادة كمية التبخر، بينما يتباين العجز المائي في أشهر الشتاء بين المحطات المذكورة. وتمت الاستعانة بالسجل المناخي لمحطات منطقة الدراسة للمدة (1988 - 2018) وقد أظهرت نتائج المطر الفعّال لهذه المدة وجود تباين في كمياته خلال الأشهر والفصول بين المحطات المناخية وهذا يعود إلى تباين عناصر المناخ (الإشعاع الشمسي، درجات الحرارة ، الرطوبة، الرياح) من منطقة لأخرى بالإضافة إلى تباين العوامل الطبيعية الأخرى، كما تم إختيار مواسم متفرقة (1989-1990) (1997-1998) (2007-2008) (2017 2018) لحساب المطر الفعّال وتوضيح إختلافه من موسم لآخر وماله من أثر على الزراعة والتدهور البيئي في محافظة نينوى .
الادخار في النهج الاقتصادي العربي الإسلامي
Authors: Flayyh Kareem Ali 
Published: October 2022

ورد الادخار في القرآن الكريم وفي نصوص كثيرة منها باللفظ الصريح كما جاء في قوله ﭧﭐﭨﭐﱡﭐ ﱩ ﱪ ﱫ ﱬ ﱭ ﱮ ﱯ ﱰ ﱱ ﱲ ﱳ ﱴ ﱵ ﱶ ﱷ ﱸ ﱹ ﱺ ﱻ ﱼ ﱽ ﱾ ﱿﲀ ﲁ ﲂ ﲃ ﲄ ﲅ ﲆ ﲇﲈ ﲉ ﲊ ﲋ ﲌ ﲍ ﲎ ﲏﲐ ﲑ ﲒ ﲓ ﲔ ﲕ ﲖ ﲗ ﲘ ﲙ ﱠ سورة آل عمران/ الآية 49 وفي مكان آخر جاء ذكر الادخار بالإشارة إلى معنى من معانيه كما في سورة طه/ الآية 131 وما يدخره المسلمون في الدنيا يعود عليه في الآخرة بالخير.
والأموال لما لها من حظوة كبيرة عندما يدخرها الإنسان ويوزعها وقت الحوائج على الناس
تعد المدخرات النقدية عند الاشخاص ثروة حقيقية يحسب لها الناس الف حساب وما يؤكد هذا هو قيام المؤسسات المالية والمصارف باعتماد اقسام وشعب شرعية لكسب اكبر قدر من الزبائن والهدف الرئيسي الذي يفكر به الناس هو الادخار والاستثمار متمسكين بالوازع الديني لتجنب الربا والحرام .
ولا يخفى على احد اهمية الادخار في حياة الاشخاص والدول عندما يكون المنقذ والسبيل الى تجاوز الكوارث والمحن وهو في حد ذاته تنظيم راق للدخل والمال ، يستند الادخار الى عوامل سلوكية وذاتية متجذره في النفس الانسانية وهذا بشكل عام يشير الى ترشيد السلوك خاصة اذا كان ضمن سياقات التوظيف الصحيح وفي هذا البحث نظهر عملية تطبيقية سليمة للادخار ضمن الاليات الفردية واليات مؤسسية لكي نكون ضمن اطار المعنى العام الشامل للادخار الذي يفضي الى عدم التصرف بهذه الاموال الا وقت الحاجة الاضطرارية وهو ما يعنينا في هذا البحث تضمن البحث. المقدمة، الادخار لغة واصطلاحاً، الادخار في القرآن الكريم والسنة النبوية، النهي عنه والمباح فيه الادخار ورأي الفقهاء فيه، الادخار والقروض، دوره في التنمية.