bluuebunny/crossref_metadata_2025_split huggingface dataset:
Speckle-type POZ protein could play a potential inhibitory role in human renal cell carcinoma
Authors: Chen Zhi , Li Zuan , Li Chunlin , Li Bingcai , Wang Haojian , Nong Deyong , Li Ximing , Huang Guihai , Lin Junhao , Li Wei 
Published: December 2022

Abstract
Background
Speckle-type POZ protein(SPOP), a substrate adaptor of Cul3 ubiquitin ligase, plays crucial roles in solid neoplasms by promoting the ubiquitination and degradation of substrates. Limited studies have shown that SPOP is overexpressed in human renal cell carcinoma (RCC) tissue. However, the exact role of SPOP in RCC remains unclear and needs to be further elucidated. The present study showed that SPOP was expressed at different levels in different RCC cell lines. The purpose of this study was to explore the roles of SPOP in the biological features of RCC cells and the expression levels of SPOP in human tissue microarray (TMA) and kidney tissues.

Methods
Here, SPOP was overexpressed by lentiviral vector transfection in ACHN and Caki-1 cells, and SPOP was knocked down in Caki-2 cells with similar transfection methods. The transfection efficiency was evaluated by quantitative PCR and western blotting analyses. The role of SPOP in the proliferation, migration, invasion and apoptosis of cell lines was determined by the MTT, wound-healing, transwell and flow cytometry assays. Moreover, the cells were treated with different drug concentrations in proliferation and apoptosis assays to investigate the effect of sunitinib and IFN-α2b on the proliferation and apoptosis of SPOP-overexpressing cells and SPOP-knockdown RCC cells. Finally, immunohistochemical staining of SPOP was performed in kidney tissues and TMAs, which included RCC tissues and corresponding adjacent normal tissues.

Results
Overexpression of SPOP inhibited cell proliferation, migration and invasion and increased cell apoptosis. Interestingly, sunitinib and IFN-α2b at several concentrations increased the proliferation inhibitory rate and total apoptosis rate of cells overexpressing SPOP. The findings of the present study showed that the SPOP protein was significantly expressed at low levels in most clear cell RCC (ccRCC) tissues and at relatively high levels in the majority of adjacent normal tissues and kidney tissues. Kaplan–Meier survival analysis showed that there was no statistically significant difference in cumulative survival based on the data of different SPOP expression levels in TMA and patients.

Conclusions
In contrast to previous studies, our findings demonstrated that overexpression of SPOP might suppress the progression of RCC cells, which was supported by cell experiments and immunohistochemical staining. SPOP could be a potential tumour inhibitor in RCC.
Notch signaling pathway: a comprehensive prognostic and gene expression profile analysis in breast cancer
Authors: Yousefi Hassan , Bahramy Afshin , Zafari Narges , Delavar Mahsa Rostamian , Nguyen Khoa , Haghi Atousa , Kandelouei Tahmineh , Vittori Cecilia , Jazireian Parham , Maleki Sajad , Imani Danyal , Moshksar Amin , Bitaraf Amirreza , Babashah Sadegh 
Published: December 2022

AbstractBreast cancer is a complex disease exhibiting a great degree of heterogeneity due to different molecular subtypes. Notch signaling regulates the differentiation of breast epithelial cells during normal development and plays a crucial role in breast cancer progression through the abnormal expression of the Notch up-and down-stream effectors. To date, there are only a few patient-centered clinical studies using datasets characterizing the role of Notch signaling pathway regulators in breast cancer; thus, we investigate the role and functionality of these factors in different subtypes using publicly available databases containing records from large studies. High-throughput genomic data and clinical information extracted from TCGA were analyzed. We performed Kaplan–Meier survival and differential gene expression analyses using the HALLMARK_NOTCH_SIGNALING gene set. To determine if epigenetic regulation of the Notch regulators contributes to their expression, we analyzed methylation levels of these factors using the TCGA HumanMethylation450 Array data. Notch receptors and ligands expression is generally associated with the tumor subtype, grade, and stage. Furthermore, we showed gene expression levels of most Notch factors were associated with DNA methylation rate. Modulating the expression levels of Notch receptors and effectors can be a potential therapeutic approach for breast cancer. As we outline herein, elucidating the novel prognostic and regulatory roles of Notch implicate this pathway as an essential mediator controlling breast cancer progression.
Rare variation: the absence of both the celiac trunk artery and the common hepatic artery
Authors: Zhang Zhenxing , Wang Shan , Ye Minfeng , Tao Feng , Guo Danling 
Published: December 2022

Abstract
Background
Knowledge of celiac trunk anatomy is important in gastrointestinal surgery, hepatopancreatobiliary surgery, transplantation and interventional radiology. Variations in the celiac trunk are common and should be predicted prior to these interventions.

Methods
A 58-year-old woman was admitted to our department for surgical treatment of gastric cancer (GC) confirmed by gastroduodenoscopy and gastric antrum biopsy. In the contrast-enhanced computed tomography (CT), we found an absence of both the celiac trunk artery (CA) and the common hepatic artery (CHA). Therefore, we used computerized three-dimensional (3D) vascular reconstruction technology to reconstruct the abdominal trunk and its branch vessels before operation.

Results
Computerized 3D vascular reconstruction confirmed an extremely rare vascular anomaly: the absence of both CA and CHA. The splenic artery (SA) and gastroduodenal artery (GDA) originated from the abdominal aorta (AA). The left gastric artery (LGA) originated from the AA directly above the junction of SA and the GDA. The left hepatic artery (LHA) originated from the left gastric artery (LGA). The right hepatic artery (RHA) originated from the superior mesenteric artery (SMA). Laparoscopic radical resection of GC was performed. This anomaly was also confirmed intraoperatively. This patient was discharged on the 10th day after surgery without any postoperative complication. There were no signs of tumor recurrence during the 6-month follow-up.

Conclusion
Correct identification of abnormal abdominal large blood vessels and their relationship with tumors before surgery is of great significance to avoid intraoperative blood vessel damage, major postoperative complications and the missing of lymph node dissection.
The stochastic frontier analysis technique in measuring the technical and economic efficiency of hospital diagnostic laboratories: a case study in Iran
Authors: Aghlmand Siamak , Feizollahzadeh Sadegh , Fathi Behrouz , Yusefzadeh Hasan , Alinejhad Mina 
Published: December 2022

AbstractAn inefficient health system wastes scarce resources even if it makes considerable gains in accountability and equity. Such a system is expected to perform better. Therefore, it is vital to examine the current performance of health systems and their constituents and assess how to reach their maximum potential. This study aimed to evaluate the technical and economic efficiency of medical diagnostic laboratories in hospitals affiliated with Urmia University of Medical Sciences (UUMS) in 2016. In this descriptive-analytical study, data from diagnostic laboratories of the hospitals of UUMS have been inputted into Frontier4.1software after taking the log of variables. Then, the technical and economic efficiency of the laboratories were obtained by estimating the production and cost function using the stochastic frontier analysis method, assuming input minimization for 2016. The mean technical and economic efficiency score of the diagnostic laboratories were determined to be 93.1% and 51.9%, respectively. These laboratories need to reduce their inputs and costs in order to achieve full efficiency without changing the amount of their output. Although the average economic efficiency of the diagnostic laboratories of the studied hospitals was high, there is still an increase in the efficiency of these units, given the cost of inputs at the time of allocating resources. In addition, it is possible to improve the technical efficiency of the clinical laboratories of hospitals affiliated with UUMS by 48.1% by applying the same level of inputs and without increasing the costs.
Y-shape osteotomy combined with subtalar arthrodesis for calcaneus malunion: a retrospective study
Authors: Bai Wenbo , Zhu Yongzhan , Xu Junkui , Liang Jingqi , Lu Jun 
Published: December 2022

Abstract
Background
This retrospective study aimed to introduce a novel method for simultaneous Y-shape osteotomy combined with subtalar arthrodesis for calcaneus malunion and to evaluate the feasibility of this method.

Methods
We retrospectively analysed the clinical and imaging data of 11 patients with calcaneus malunion treated using Y-shape osteotomy and subtalar arthrodesis who were admitted to our hospital from June 2018 to October 2020. The patients included 9 males and 2 females aged from 24 to 69 years old, with an average age of 42.18 years. The clinical and radiological results were assessed with the Visual Analogue Scale (VAS) pain score and American Orthopaedic Foot and Ankle Society (AOFAS) hindfoot score. In addition, functional recovery and general quality of life were evaluated using the 12-Item Short-Form Survey (SF-12).

Results
All radiological parameters were significantly improved at the last follow-up, with increases of 14.37°, 9.18°, and 4.51 mm in the Böhler’s angle, calcaneal pitch angle, and talocalcaneal height, respectively, and decreases of 6.39 mm and 6.18° in the calcaneal width and Hindfoot alignment angle (p < 0.05). The mean AOFAS and VAS scores at the last follow-up improved compared with those preoperatively, from 34.18 ± 9.53 to 84.18 ± 11.59 and from 6.90 ± 1.22 to 1.90 ± 1.13, respectively (p < 0.05). The SF-12 physical and mental health scores were 49.65 ± 6.84 and 52.68 ± 7.88, respectively. Furthermore, the early postoperative complications included skin necrosis in one and sural neuralgia in one patient, and the late postoperative complication included ankle pain in one patient. No other complications, such as implant discomforts, malunion, nonunion and re-fracture, were presented.

Conclusion
These results indicate that Y-shape osteotomy combined with subtalar arthrodesis is an effective new method for the treatment of calcaneal malunion. Advantages include improvement of the anatomic shape of the calcaneus and union rates for subtalar arthrodesis.
Development and psychometric evaluation of the CanSmart questionnaire to measure chronic disease self-management tasks
Authors: Lambert Sylvie D. , Bartlett Susan J. , McCusker Jane , Yaffe Mark , Ciampi Antonio , Belzile Eric , de Raad Manon 
Published: December 2022

Abstract
Background
Psychometrically sound measures of chronic disease self-management tasks are needed to improve identification of patient needs and to tailor self-management programs. This study aimed to develop and conduct a preliminary psychometric analysis of the CanSMART questionnaire among a diverse, multimorbid Canadian population.

Methods
The data were drawn from a cross-sectional online survey to examine self-management needs and support preferences. Participants were 306 Canadian adults with one or more physical and/or emotional chronic conditions. The questionnaire on frequency of self-management tasks was developed with substantial patient partner input. We conducted Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA) of the 11 self-management tasks comprising the scale in two randomly selected subsamples, followed by Rasch analysis. Associations between patient characteristics and the self-management task subscales and individual items were explored.

Results
The factor analyses identified two self-management task subscales that were labelled Coping tasks (6 items) and Physical tasks (3 items), with Cronbach’s alpha of 0.70 and 0.67, respectively. Rasch analysis suggested that participants had difficulty discriminating between response options “mostly” and “always”. In analyses of independent associations with patient characteristics, both Coping and Physical tasks were associated with reporting more than one chronic disease and employment disability. The Coping tasks subscale was associated with female sex. Two items, on medication use and monitoring biological parameters, did not load on either scale. Both were associated with specific diagnoses.

Conclusions
In this preliminary analysis, two self-management tasks subscales exhibit good psychometric properties. Two items that did not load on either scale may represent additional dimensions of self-management. This work provides the basis for further scale development and use in research and clinical practice.
Anti-drug antibodies and rheumatoid factor level in patients with rheumatoid arthritis using the infliximab biosimilar CT-P13
Authors: Sakane Hideo , Okamura Koichi , Inoue Makoto , Inoue Hiroshi , Yonemoto Yukio , Mitomi Hirofumi , Tsuchida Kosei , Suto Takahito , Kaneko Tetsuya , Chikuda Hirotaka 
Published: December 2022

Abstract
Background
This study evaluated the existence of anti-drug antibodies (ADAs) before and 52 weeks after switching from intravenous infliximab (IFX) to intravenous CT-P13 in patients with rheumatoid arthritis (RA).

Methods
We performed a prospective observational study. Twenty-eight patients (7 males and 21 females) received intravenous CT-P13 after intravenous IFX, and the clinical data were collected from medical records. Rheumatoid factor (RF) and anti-CCP antibody were examined at baseline. At baseline and 52 weeks after the start of CT-P13 treatment, the Disease Activity Score based on the 28-joint count and the levels of C-reactive protein, matrix metalloproteinase-3, and ADA, as well as the erythrocyte sedimentation rate were evaluated. ADAs were measured using an enzyme-linked immunosorbent assay kit.

Results
Seven (25%) and 6 (21.4%) cases were positive for ADAs at baseline and 52 weeks after, respectively. One case became newly positive for ADAs at week 52. Two of the ADA-positive cases became ADA-negative 52 weeks after. The ADA-positive group showed significantly higher RF values at baseline than the ADA-negative group (p = 0.03). No difference was observed between the ADA-positive group and the ADA-negative group regarding other clinical parameters.

Conclusions
The positive rate of ADAs did not increase after switching from intravenous IFX to intravenous CT-P13. Among the patients with ADAs, a high level of RF was observed at baseline.
Determination of age reference standards based on mandibular third molar root development in a Ugandan population aged 10–22 years
Authors: Mutebi Annet Kutesa , Kamulegeya Adriane , Nabaggala Grace , Mwesigwa Cathy Lutalo 
Published: December 2022

AbstractBackgroundThis study was aimed at establishing age estimates based on distal root development of the mandibular third molar for Ugandans aged 10–22 years. This was a cross-sectional study using orthopantomograms (OPGs) of 671 patients attending the Mulago Hospital Dental Clinic. The patients’ chronological age and sex were obtained from either their national identity cards or birth certificates (females;n= 326, 48.6%). Third molar root development was assessed using Demirjian, Goldstein, and Tanner (DGT), modified Demirjian by Solari, Moorrees, Fanning, and Hunt (MFH), and Haavikko methods. Age was summarized using means/standard deviation (SD), medians, and lower and upper quartiles. Sex differences were assessed using Student’st-test.ResultsCrown completion (stage D) and root initiation (stage Ri) were observed at 12.6 years for females and 13.5 years for males (P= 0.02), while complete apex closure occurred at 19.8 for females and 20.1 for males (P= 0.3). There were statistically significant differences in Demirjian root stages E, F, and G and MFH and Haavikko stages Ri, R1/4, and R1/2between the sexes (P< 0.05). The difference in the mean age of root development between females and males ranged between 0.9 year at DGT/MFH root stages D and Ri(13.5–12.6) and 1.4 years at Solari, MFH, and Haavikko root stages F, R1/4, and R1/2(16.3–14.9, 17.1–15.7). No differences were observed between the right and left mandibular molars.ConclusionsThis study provides age reference standards based on third molar root development specific to the Ugandan adolescent and young adult population. The findings can be used to formulate contemporary standards and utilized as reference material to assess third molar maturity for forensic purposes.
Scoping Review to Inform the Future Development of a Measure for Team-Based Care in Oncology
Authors: Zunich Rada , Roberts Megan C. , Blanchard Carrie , Muluneh Benyam , Carlson Rebecca Beth , Helms Taylor 
Published: December 2022

PURPOSE: Team-based care is the delivery of health services to an individual by at least two health care providers working collaboratively to achieve optimal care. Participants on the National Cancer Institute and the ASCO Teams in the Cancer Care Delivery Project have defined 13 key principles to serve as the foundation for a successful team; however, it is unclear whether there exist measures of these key principles. METHODS: A scoping literature search was conducted for each key principle on PubMed and Embase to identify existing measures for key principles. Articles of interest were exported to a citation manager, Sciwheel, cataloged by the key principle. Existing measures were extracted via a two-stage screening process, with an abstract review followed by a full-text review. RESULTS: Fifteen unique measures were identified, with items extrapolated for 12 of the 13 key principles. Measures were not exclusive and could represent more than one key principle. The number of measures varied per principle from zero to five, with Team Composition and Diversity yielding no existing measure. CONCLUSION: The long-term goal is to compile and edit these measures, to create a comprehensive measure to be used in various team-based oncology care settings, and to address areas for improvement, ultimately optimizing patient care.
MRI and the control of electromagnetic fields at work regulations 2016 – professional body guidance
Authors: Bainbridge Alan , Keevil Stephen 
Published: December 2022

The Control of Electromagnetic Fields at Work Regulations (2016) implement the requirements of the EU Physical Agents (Electromagnetic Fields) Directive 2013 into UK Law. These regulations prohibit exposure of employees to electromagnetic fields above defined exposure limit values (ELVs). However, for MRI work activities, there is an exemption from this requirement provided that exposures are reduced to a level that is as low as is reasonably practicable. There are additional requirements for an adequate risk assessment, provision for staff training and monitoring of any health effects. This document provides specific guidance on achieving compliance with the regulations in the MRI setting. Consideration of what level of exposure is ‘reasonable’ is key to the proper application of these regulations. The ELVs, as defined in the regulations, are presented in terms of their relevance to exposures in the MRI-setting, what the direct and indirect effects of exposures are likely to be and what reasonable control measures can be put in place. Worked examples provide a step-by-step guide to achieving compliance with the regulations. For typical MRI working practices, it is unlikely that any permanent health effects will be experienced. Indeed, any effects that are experienced are likely to be transient and have no long-term consequences. Control measures to minimise the risks to workers in the MRI environment should already be in place in well-run units and there should be no need to implement additional control measures for exposures above the ELVs.
Prevalence and correlates of neurocognitive impairment and psychiatric disorders among schoolchildren in Wakiso District, Uganda: a cross-sectional study
Authors: Nampijja Margaret , Sembajjwe Wilber , Mpairwe Harriet , Mpango Richard , Kinyanda Eugene 
Published: December 2022

Background: There is limited data on the burden of mental disorders among children in the general population in Africa. We examined the prevalence and correlates of neurocognitive and psychiatric disorders among schoolchildren in Uganda. Methods: This cross-sectional study enrolled 322 schoolchildren aged 5-17years in Wakiso, Uganda. We assessed for neurocognitive impairment using the Kaufmann-Assessment-Battery, and psychiatric disorders (major-depressive-disorder (MDD), attention-deficit-hyperactivity-disorder (ADHD), generalised-anxiety-disorder (GAD), and substance-use-disorder (SUD)) using the parent version of the Child and Adolescent Symptom Inventory-5, and Youth Inventory-4R Self Report. Prevalence and risk factors were determined using respectively descriptive statistics, and univariable and multivariable logistic regression. Results: Twenty-five participants (8%) had neurocognitive impairment. Nineteen (5.9%) participants had MDD, nine (2.8%) had ADHD, seven (2.2%) had GAD, 14 (8.6%) had SUD; and 30 (9.3%) had any psychiatric disorder. Among the exposure variables examined in this study, including asthma, age, sex, grade of schooling, type of school and maternal and father’s education and family socio-economic status, only asthma was associated with the disorders (MDD). Conclusions: The relatively high burden of mental disorders in this general population of children warrants targeted screening of those at risk, and treatment of those affected. Further, future studies should extensively investigate the factors that underlie the identified psychiatric disorders in this and similar general populations.
O ESTADO ESTRATÉGICO E A PARTICIPAÇÃO SOCIAL DO TERCEIRO SETOR
Authors: Faria Andrade Silva Alan , Villas Bôas Regina Vera 
Published: December 2022

No Terceiro Setor encontramos diversas formas da atuação das associações e fundações na sociedade brasileira, as quais visam à efetividade dos direitos fundamentais e dos direitos humanos, por meio da permissão ou não interferência do Estado. Para aprofundar essas formas de atuação e relação ao Estado, a pesquisa objetivou-se neste estudo estabelecer conceitos sobre o que é o Terceiro Setor, a sua composição e respaldo jurídico perante o ordenamento jurídico brasileiro, bem como analisar estudos relacionados ao Estado Democrático de Direito e às contribuições que agentes da sociedade civil – como movimentos sociais e organizações do Terceiro Setor – deram na compilação da Constituição Federal de 1988. Foi necessário também mergulhar nos estudos de Naves sobre o Estado Estratégico. Verificou-se que houve contribuições significativas tanto dos movimentos sociais como de agentes do Terceiro Setor no período da redemocratização do Brasil – anos 80/90 –, possibilitando, assim, maior participação da sociedade civil nos mecanismos de poder do Estado brasileiro. Ocorreu, então, a possibilidade de tecer mais uma conceituação de Estado, qual seja, o Estado Estratégico. Para abordar essas questões, o referencial utilizado foi a pesquisa bibliográfica disponível na seara jurídica e demais áreas do conhecimento quanto ao Terceiro Setor e ao Estado Democrático de Direito. Resultou, assim, no entendimento de que Estado Estratégico não é mais um conceito atribuído ao Estado, mas uma atitude, a partir do conceito de Estado Democrático de Direito, que gerencia a participação de vários atores sociais e inclusive a sua própria atuação. 10.12957/rfd.2020.38492