bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does the Modality of Renal Replacement Therapy Affect Gastrointestinal Symptoms and Findings?
Authors: KARAHAN Doğu , ŞAHİN İdris 
Published: March 2022

Abstract
OBJECTİVE: Chronic kidney disease (CKD) affects gastrointestinal system (GIS) and causes histological, functional and mucosal changes. There are scarce data investigating GIS symptoms and findings in patients with CKD stage III-V, receiving hemodialysis (HD) and peritoneal dialysis (PD). In this study, we aimed to evaluate the frequency of gastrointestinal symptoms and findings and effect of renal replacement therapy on GIS.METHOD: A total of 300 patients (97 in CKD stage III-V, 92 PD, 101 HD) included to this study. Gastrointestinal complaints and diseases, background characteristics of patients, drugs they used were questioned by interviews, forms were filled and examinations of patients were performed. Results of upper GIS endoscopy, colonoscopy, abdominal ultrasonography and tomography of patients were evaluated.RESULTS: The most common complaints were dyspepsia (50%), nausea (45%), epigastric pain (44%) among all patients, generally. Gastrointestinal disorders like gastritis (62%), gastroesophageal reflux (39%) were frequent in patients. Ratio of patients with weight loss was 20% in predialysis and 8% in PD and the ratio was higher in predialysis group significantly (p=0,016). It is found that gastritis ratio 70% in PD, 55% in HD, hemorrhoids ratio 24% in PD, 12% in HD. Gastritis and hemorrhoids ratio was higher in the PD group than in the HD group significantly (p=0.043,p= 0.028), otherwise there wasn’t difference between PD and predialysis group, significantly.CONCLUSİON: This study showed that; gastrointestinal symptoms and disorders were very common in CKD, beside of this; while gastritis and hemorrhoids were more frequent in the PD, esophagitis and hiatal hernia were more frequent in the HD.
siRNA-mediated gene knockdown via electroporation in hydrozoan jellyfish embryos
Authors: Nakajima Yu-ichiro , Masuda-Ozawa Tokiha , Fujita Sosuke , Nakamura Ryotaro , Watanabe Hiroshi , Kuranaga Erina 
Published: March 2022

Abstract
As the sister group to bilaterians, cnidarians stand in a unique phylogenetic position that provides insights into evolutionary aspects of animal development, physiology, and behavior. While cnidarians are classified into two types, sessile polyps and free-swimming medusae, most studies at the cellular and molecular levels have been conducted on representative polyp-type cnidarians and have established techniques of genetic manipulation. Recently, gene knockdown by delivery of short hairpin RNAs into eggs via electroporation has been introduced to two polyp-type cnidarians, Nematostella vectensis and Hydractinia symbiolongicarpus, enabling systematic loss-of-function experiments. By contrast, current methods of genetic manipulation for medusa-type cnidarians, or jellyfish, are quite limited, and reliable techniques are required to interrogate function of specific genes. Here, we present a method to knockdown target genes by small interfering RNA (siRNA) delivery into fertilized eggs via electroporation, using the hydrozoan jellyfish, Clytia hemisphaerica and Cladonema paciificum. We show that siRNAs targeting endogenous GFP1 and Wnt3 in Clytia efficiently knockdown gene expression and result in known planula phenotypes: loss of green fluorescence and defects in axial patterning, respectively. We also successfully knock down endogenous Wnt3 in Cladonema by siRNAs electroporation, which circumvents the technical difficulty of microinjecting small eggs. Unexpectedly, Wnt3 knockdown in Cladonema does not induce morphological defects in planula, suggesting a distinct mechanism that operates during embryogenesis. Our method for electroporation of siRNAs targeting specific genes is applicable to other animals, including and beyond jellyfish species, and will facilitate the investigation and understanding of myriad aspects of animal development.
Antitumor effect of polyphyllin D on liver metastases of neuroblastoma
Authors: Kondo Yasuhiro , Watanabe Shunsuke , Naoe Atsuki , Takeuchi Toshiyuki , Niimi Atsuko , Suzuki Motoshi , Asai Naoya , Okada Seiji , Tsuchiya Tomonori , Murayama Mika , Yasui Toshihiro , Inoue Mikihiro , Suzuki Tatsuya 
Published: March 2022

Abstract
PurposeWe previously reported that polyphyllin D, a main component of the traditional Chinese medicinal herb Paris polyphylla, exhibited anticancer effects in vitro against human neuroblastoma cells. The aim of this investigation was to examine the presence or absence of in vivo anti-metastasis effects of polyphyllin D.MethodsSubcutaneous and intraperitoneal tumors, and metastasis models were established in immune-deficient BALB/c nude and BALB/c Rag-2/Jak3 double-deficient (BRJ) mice using the human neuroblastoma cell lines IMR-32, LA-N-2, or NB-69. For evaluating polyphyllin D activity, we used a mouse model of metastasis of the IMR-32 cell line and analyzed the livers of the phosphate buffer solution- and polyphyllin D- treated groups.ResultsLiver metastasis and intraperitoneal dissemination models were successfully established in immune-deficient BRJ mice using the three human neuroblastoma cell lines. In the liver metastasis model, we found that polyphyllin D suppressed both the number and total area of metastatic foci.ConclusionsWe developed a mouse model of neuroblastoma metastasis and demonstrated for the first time that polyphyllin D has an antitumor effect on neuroblastoma liver metastases.
Integrated Bioinformatics Analysis Reveals Marker Genes and Immune Infiltration for Pulmonary Arterial Hypertension
Authors: Tang Shengxin , Zeng Xiaojuan , Liu Yue , Liu Bin 
Published: March 2022

Abstract
Background
It is well known that Pulmonary arterial hypertension (PAH) is a chronic cardiopulmonary syndrome with high pulmonary vascular load and eventually causing RV heart failure even death. This research is aimed to detect the underlying key genes and potential mechanism of PAH using some kinds of bioinformatic tools.
Methods
The gene microarrays GSE22356, GSE131793 and GSE168905 were acquired from the GEO. Subsequently, a host of bioinformatics techniques, for instance, DAVID for GO annotation and KEGG enrichment analysis, STING for PPI, Cytoscape software etc. were utilized to investigate DEGs between PAH and healthy controls. Additionally, we also predicted the transcription factors regulating DEGs. And CIBERSORT was used to conduct immune infiltration analysis.
Results
we identified 403 up-regulated DEGs and 874 down-regulated DEGs in peripheral blood samples between 32 PAH patients and 29 controls. These functional changes of DEGs were mainly enriched in protein binding, extracellular exosome, extracellular space, extracellular region and integral component of plasma membrane. SLC4A1, AHSP, ALAS2, CA1, HBD, SNCA, HBM, SELENBP1, SERPINE1 and ITGA2B were detected as hub genes. These key genes are chiefly enriched at extracellular exosome, hemoglobin complex, blood microparticle, oxygen transporter activity. Among TF-DEGs network, 42 target DEGs and 6 TFs were predicted with an NES > 4 (TEAD4, TGIF2LY, GATA5, GATA1, GATA2, FOS). Immune infiltration analysis showed that monocytes occupied the largest proportion of immune cells.
Conclusion
We detected SLC4A1, AHSP, ALAS2, CA1, HBD, SNCA, HBM, SELENBP1, SERPINE1 and ITGA2B as the most significant markers of PAH. The PAH patients had higher infiltration of NK cell activation, monocyte, T cell CD4 memory activation, and mast cell than healthy controls and lower infiltration of T cell CD4 naive. These identified genes and these immune cells probably have precise regulatory relationships in the development of PAH.
Heart Disease Prediction Using Scaled Conjugate Gradient Back Propagation of Artificial Neural Network
Authors: PAUL BANIBRATA , Karn Bhaskar 
Published: March 2022

Abstract
Heart disease is a deadly disease in human life. The mortality rate from any disease is the highest in the world. Therefore, before reaching the final stage of this heart disease, all precautionary measures must be taken. For this reason without the help of any kind of traditional methods, if we can scientifically diagnose heart disease at an early stage through various decision support systems, then surely death rate of this disease will decrease in the whole world. Many researchers investigate the diagnosis of heart disease by creating various intelligent medical decision support systems. Artificial neural network concepts represent the highest predictive accuracy over medical data compared to other decision support systems. In this paper we propose a better prediction method for the existence of heart disease through the scaled conjugate gradient back propagation of artificial neural networks using K-fold cross validation. For cardiac datasets, the University of California Irvine (UCI) Machine Learning Repository and IEEE data port have been used. For Cleveland processed heart dataset, the proposed system uses 13 input attributes and provides minimum 63.3803% & maximum 100% accurate results similarly for Cleveland Hungarian Statlog heart dataset the proposed system uses 11 input attributes and provides minimum 88.4754% & maximum 100% accurate results by estimating the presence and absence of heart disease during testing.
Interaction of Diabetes Mellitus and Chronic Kidney Disease on Outcome in Heart Failure with Reduced Ejection Fraction
Authors: Yang Yanfang , Huang Zhidong , Wu Bo , Lu Jin , Xiu Jiaming , Tu Jiabin , Pan Yuxiong , Bao Kunming , Wang Junjie , Chen Weihua , Liu Jin , Chen Shiqun , Chen Kaihong , Chen Liling 
Published: March 2022

Abstract
Background
Diabetes mellitus (DM) is common in patients with heart failure (HF). But the harmful effect of DM on mortality in patients with HF remains controversial. Furthermore, it seems that no consistent conclusion on whether chronic kidney disease (CKD) modifies the relationship of DM and poor prognosis in patients with HF.
Methods
We analyzed the individuals with heart failure with reduced ejection fraction (HFrEF) from the Cardiorenal ImprovemeNt (CIN) cohort between January 2007 to December 2018. The primary endpoint was all-cause mortality. The patients were divided into four groups (control vs. DM alone vs. CKD alone vs. DM and CKD). Multivariate Cox proportional hazards analysis was conducted to examine the association among DM, CKD and all-cause mortality.
Results
There were 3,273 patients included in this study (mean age: 62.7 ± 10.9 years, 20.4% were female). During a median follow-up of 5.0 years (interquartile range: 3.0 to 7.6 years), 740 (22.6%) patients died. Patients with DM have a higher risk of all-cause mortality (HR [95% confidence interval (CI)]:1.32[1.10–1.57]) than those without DM. In patients with CKD, DM had a 61% (HR [95% CI]:1.61[1.26–2.06]) increased adjusted risk of death relative to non-DM, while in patients with non-CKD, there was no significantly difference in risk of all-cause mortality (HR [95% CI]:1.01[0.77–1.32]) between DM and non-DM (p for interaction = 0.013).
Conclusions
Diabetes is a potent risk factor for mortality in patients with HFrEF. Furthermore, DM had a substantially different effect on all-cause mortality depending on CKD. The association between DM and all-cause mortality was only observed in patients with CKD.
WITHDRAWN: Factors Influencing Residents' Satisfaction Under Multiple Sources of Environmental Pollution: A Structural Equation Modelling Approach
Authors: Pham Thoa Thi Kim , Tran Phuong Thi Minh , Nguyen Teron 
Published: March 2022

Abstract
Residents living nearby multiple sources of environmental pollution are suffering from adverse health effects and many other factors. However, very few studies focus on the complex interaction between multiple effects on residents' satisfaction. This study reports a case study in Phuoc Tuong quarry and Khanh Son landfill in Danang city, Vietnam, the two environmental hotspots hindering city development toward sustainability. Using Structural Equation Modelling (SEM) approach and 420 valid questionnaires, the relationship between measured variables and latent constructs was analyzed and established. Due to the wider spread of influencing area, the effects of Phuoc Tuong quarry are higher than Khanh Son landfill (path coefficient |ß| = 0.265 > 0.161), whereas, living distance (ß = 0.165) and residents' demographics (|ß| = 0.134) are also contributing to residents' satisfaction with business, health and life in overall. Among them, living distance from the pollution sources is confirmed to have a positive influence while the rest constructs negatively influence residents' satisfaction. The satisfaction, in turn, has a direct negative influence on residents' moving intention, meaning residents desire to resettle their living locations when dissatisfied with the current living conditions. Based on the study findings, practical applications can be focused more efficiently on appropriate measures to reduce the harmful effects of the quarry as compared to the landfill. Also, urgent measures should also be focused on the resident group living within 1 km from both pollution sources.
HPV16 Status Predicts Differential Interactome in Head and Neck Squamous Cell Carcinoma: Potential Prognostic Biomarkers and Therapeutics in Framework of 3P Medicine
Authors: Kori Medi , Arga Kazim Yalcin 
Published: March 2022

Abstract
Human papillomavirus (HPV) infection is one of the causative factors in the development of head and neck squamous cell carcinoma (HNSC). One in four HNSC cases is positive for HPV infection, and HPV16 is the most common infection in HNSC cancer. HPV-positive and HPV-negative HNSC patients differ significantly in their molecular profiles and clinical features. Although the diagnosis and treatment strategies of HNSC patients should differ depending on the HPV status of the patient, there are no specific treatment regimens for these patients today. Given the enormous variation in HNSC cancers depending on HPV status, the 3P framework is of great importance specifically for these patients. In this study we present systemic biomarkers and treatment options tailored to the patient's HPV status. Gene expression levels of HPV16-positive and -negative cancer patients were used as proxies, and the differential interactome algorithm was employed to identify the differential interacting proteins (DIPs) around which significant changes occur at protein-protein interactome level. By assessing the prognostic capabilities and druggabilities of DIPs and their interacting partners (DIP-centered modules), we were able to introduce eight DIP-centered modules as potential systems biomarkers specialized for either HPV16-positive or HPV16-negative phenotype. The prognostic power of three modules specific for HPV16-positive phenotype was also validated by an independent cohort study. Finally, raloxifene was repositioned as a drug candidate for the treatment of HPV16-positive HNSC patients. With this study, we open new perspectives for the development of effective prognostic and treatment strategies for HNSC patients depending on their HPV16 status.
Consecutive transcutaneous tissue oxygen measurement for determining peripheral revascularization and correlation to ischemic diabetic ulcers outcome
Authors: Sun Haoyi , Wu Yichun , Sung Lichin , Lin Xinyi , Tsai Fengchou , Lin Yenkuang , Tam Kawai , Chang Shuncheng 
Published: March 2022

Abstract
BACKGROUND
The ankle-brachial index (ABI) might underestimate the prevalence of peripheral vascular disease (PVD) in patients with diabetic foot ulcers (DFUs). This study manifested the effectiveness of transcutaneous oxygen pressure (TcPO2) in determining whether PVD should be treated with revascularization.
METHODS
100 patients with diabetic foot ulcers underwent ABI and TcPO2 measurement and were divided into the vascular or nonvascular reconstruction group. TcPO2 and ABI were consecutively measured 4 times within 6 months in patients with DFUs. Differences in TcPO2 values up to 6 months were compared between vascular reconstruction and non-reconstruction groups and patients with and without end-stage renal disease (ESRD).
RESULTS
Of 100 patients with diabetic foot ulcers, 51 who had abnormal transcutaneous oxygen pressure values underwent vascular reconstruction. TcPO2, but not the ABI, measured before treatment significantly differed between the 2 groups. TcPO2 values significantly increased after vascular reconstruction. TcPO2 values gradually decreased during the 6-month follow-up after vascular reconstruction; however, the ABI tended to increase. In patients with ESRD, transcutaneous oxygen pressure values declined significantly and rapidly within 3 months. TcPO2 values before vascular reconstruction were significantly lower in patients receiving amputation than those receiving limb salvage surgery.
CONCLUSIONS AND RELEVANCE:
Compared with ABI, TcPO2 measurement is a more accurate and appropriate method for evaluating ischemic limb perfusion and the necessity of revascularization. TcPO2 measurement should be used as the major follow-up tool, and patients with end-stage renal disease should undergo more follow-ups.
TRIAL REGISTRATION:
Joint Institutional Review Board of Taipei Medical University (N201701054 and N201805105).
The effect of Ethyl methanesulfonate and Methylsalicylate induced on physiological and biochemical characteristics of rice OMCS2000 in anaerobic germination and plant growth under waterlogging
Authors: Dat Luu Khai , Thong Le Minh , Hien Pham Thi Thu , Loi Tran Thuy , Ha Pham Thi Thu 
Published: March 2022

Abstract
Background
The use of direct seeding in lowland rice ecosystems brings many advantages for cultivators such as reducing labor costs, effective water management, early harvesting, and less dependence on herbicides, thus improving tolerance to submergence during germination in rice is the best suggestion. In this study, rice seeds of variety OMCS2000 were treated with different concentrations of Ethyl-methane sulphonate (EMS) (0.1% and 0.125%) and concentration of Methylsalicylate (MeSA) (0.1 mM and 0.125 mM) to examine the positive and negative impact on seed germination, plant height, root length, α-amylase activity, phenolic, and flavonoid content under various water levels for submerged conditions (0, 5, 10, and 15 cm) of plants at 14 days.
Results
Our data showed that 0.1% EMS significantly increased the germination rate, germination vigor index, morphological (root and shoot lengths), and alpha amylase of rice under waterlogging while MeSA significantly reduced them. But we also recognized that MeSA (0.1 mM) significantly improved in phenolic and flavonoid contents of rice under waterlogging.
Conclusion
The treatment with 0.1% EMS showed the highest tolerance to anaerobic conditions which could be recommended to apply in a breeding program new OMCS2000 line for waterlogging tolerance.
Intervention Efficacy of Off-Label use of Alprostadil Lipid Microsphere Injection on Neurosurgical Patients: A Retrospective Before-after Study
Authors: Luo Yuling , Fan Qingze , Yu Yongqi , Zhang Lunhui , Dong Limei , Luo Hongli 
Published: March 2022

Abstract
Background and purpose: As an adjuvant drug, alprostadil lipid microsphere injection (Lipo-PGE1) is one of the best-selling drugs in China in recent years. However, the off-label use of Lipo-PGE1 is very common. This study aimed to investigate the use of Lipo-PGE1 and evaluate the clinical effects and economic benefits after administrative intervention on inappropriate use of Lipo-PGE1 in neurosurgical patients in a Chinese tertiary hospital. Methods: Administrative interventions were implemented from January to December 2018 by reducing the procurement volume of Lipo-PGE1, judging the rationality of medical records, and establishing reward and punishment mechanism. Results: Administrative interventions significantly decreased prescription rate (49.98% vs 22.49%), utilization (22311 DDDs vs 8334 DDDs), drug use density (43.52 DDDs/TID vs 15.84 DDDs/TID), total expenditure (3.58 million RMB vs 1.30 million RMB), and average expenditure (2025.04 RMB vs 1466.49 RMB) of Lipo-PGE1. To our delight, these intervention effects were maintained or even better in the 1-year post-intervention period. Moreover, in the intervention and post-intervention phases, the Lipo-PGE1 use for no indications as well as inappropriate drug dose, frequency, menstruum type, combination and contraindication were markedly reduced. Besides, the mean costs (P < 0.001), and mean duration (P < 0.001) of Lipo-PGE1 were also obviously decreased. Conclusions: The administrative intervention obviously reduced the off-label use of Lipo-PGE1. However, there still remains a number of inappropriate uses of Lipo-PGE1. To further improve rational use of Lipo-PGE1, the combination of administrative intervention and real-time clinical pharmacists intervention should be implemented.
An early relapse-associated gene signature optimizes prognostic prediction for gastric cancer patients
Authors: Wang Pengchao , Song Qingyu , Xia Qingcheng , Qiu Shipei , Tang Xin , Lu Ming , Zhao Qinghong , Ma Xiang 
Published: March 2022

Abstract
Background: Gastric cancer (GC) is one of the most common cancers in the world. Patients with GC who experienced early relapse have poor prognosis. We aim to develop an early relapse-associated gene signature to optimize prognosis prediction in patients with replapsing GC. Methods: The GC cohorts including GSE62254 set (N=300) and GSE15459 set (N=192) were extracted from Gene Expression Omnibus (GEO) database. Propensity score matching (1:1) based on pathological stage was executed between patients with early relapse and long-term survival from GSE62254 set. Global transcriptome analysis was performed between the two groups to identify early relapse-associated gene. Based on the differentially expressed genes, we developed a classifier incorporating 5 genes that using LASSO Cox regression model. The signature’s prognostic value was internally validated in 210 GC patients and validated in GSE15459 set externally. The patients from GSE62254 set could be divided into high-risk or low-risk group.Results: In the train set, patients in high-risk group had poor prognosis as compared to those in low-risk group [hazard ratio (HR): 3.002, 95% confidence interval (CI): 2.132-4.226, P<0.001)]. Good reproducibility for the prognostic value of early relapse-associated gene signature was verified in the internal validation set (HR: 2.772, 95% CI: 1.836–4.184, P<0.001) and another external validation set (HR: 1.733, 95% CI: 1.149–2.614, P=0.009). Also, we developed a nomogram which integrated the five mRNA classifier, pathological stage and lymph node ratio (LNR) to evaluate prognosis based on GSE62254 set. Time-dependent receiver-operating characteristic at 1 year demonstrated that integrated signature had better prognostic accuracy [area under curve (AUC=0.849)] than the American Joint Commission on Cancer TNM staging system (AUC=0.773) and LNR (AUC=0.811) in GSE62254 set. Conclusions: This study suggest that an early relapse-associated gene signature that can robustly divide gastric cancer patients into two groups with distinctive prognosis. This classifier may contribute to select gastric cancer patients with poor prognosis who require more frequent follow-up and more aggressive therapeutic intervention.