bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does the Covid-19 pandemic have an effect on wound culture in patients undergoing appendectomy? A Case Control Study
Authors: BOLAT Hacı , AVAN MUTLU Tuğba 
Published: March 2022

Aim: The aim of this study is to examine the effect of the changing microbiota structure during the pandemic period on the bacterial aerobic culture profile of the wound taken from patients operated for acute appendicitis, together with clinical variables.
Materials and Methods: Our study included 125 patients who underwent an appendectomy in the General Surgery Clinic between 01.03.2019-01.02.2021 and whose wound culture was taken during an appendectomy. The patients were divided into two groups; group1 (pre-pandemic) and group2 (during a pandemic). Both groups were compared in terms of age, gender, clinical, laboratory and wound culture data.
Results: There was no significant difference between the groups in terms of age, clinical symptoms, surgical method, laboratory and radiological data (p>0.05). In the pre-pandemic group, there was growth of E. coli in 35 (53%) patients, K. pneumoniae in 3 (4.5%) patients, and P. aeruginosae in 2 (3) patients from Gram-negative bacteria while Strep. anginosus and Strep. constellatus growth were most common from Gram-positive bacteria. In the pandemic group, there was the growth of E. coli in 29 (49.2%) patients, P. aeruginosae in 5 (8.5%) patients, K. pneumoniae in 2 (3.4%) patients from Gram-negative bacteria, Citrobacterfreundii and Streptococcusanginosus growth were the most common from Gram-positive bacteria. Escherichiacoli is the most common bacteria in both groups.
Conclusion: Although E. coli was found to be the most frequently identified microorganism in patients with acute appendicitis, an increase in the density and resistance of Pseudomonas group bacteria were detected, possibly due to the effect of the COVID-19 pandemic.
NÚCLEO AMPLIADO DE SAÚDE DA FAMÍLIA E ATENÇÃO BÁSICA: FORMAÇÃO E ATUAÇÃO EM SAÚDE
Authors: Benvindo Vinícius Vieira , Nunes Lélia Cápua , Almeida Nizia Araujo Vieira 
Published: March 2022

Objetivo. Caracterizar o perfil, formação e a atuação dos profissionais integrantes das equipes dos Núcleos Ampliados de Saúde da Família e Atenção Básica (NASF-AB), do munícipio de Governador Valadares - Minas Gerais e investigar o conhecimento desses profissionais sobre o programa e suas diretrizes.Métodos. Tratou-se de um estudo descritivo, realizado por meio da aplicação de um questionário estruturado, preenchido por 82,9% dos integrantes do programa.Resultados. A maioria teve formação em universidades/faculdades privadas (94,1%), não realizaram nenhuma atividade prática curricular em Saúde Pública, exceto o estágio curricular obrigatório. O ingresso no programa ocorreu principalmente pela análise de currículo (47,1%). Verificou-se também mesmo percentual de profissionais sem experiência profissional em Saúde Pública prévia ao ingresso no programa. As diretrizes do NASF-AB mais citadas pelos profissionais foram as relacionadas à Promoção a Saúde (82,4%) e à Interdisciplinaridade (73,5%) e as menos referidas foram a Educação Permanente em Saúde (14,7%) e a Participação Social (17,6%). Metade dos profissionais relataram, uma boa integração entre as equipes do NASF-AB e as da Estratégia de Saúde da Família. Em relação ao planejamento em saúde em cada território foi identificada reduzida utilização de instrumentos para o planejamento (53%) e incipiente participação popular nas decisões de saúde e planejamento para o território (44%).Conclusões. Os achados desse estudo demonstram uma lacuna na formação de profissionais em saúde coletiva na graduação e em serviço para atuação no NASF-AB, o que aponta para a necessidade de aperfeiçoamento da formação em saúde e maior investimento nas ações de Educação Permanente.
Uso de tablets por Agentes Comunitários de Saúde no Brasil
Authors: Leandro Bianca Borges da Silva , Rangel Juliana Felício , Pinto José Mauro Da Conceição , Lopes Reinaldo De Araújo Dantas , Martins Fernanda Do Nascimento 
Published: March 2022

Objetivo: Refletir sobre o uso institucionalizado de tablets pelos AgentesComunitários de Saúde (ACS) no seu processo de trabalho. Método: Pesquisa exploratória, com características quantitativa e qualitativa. Busca de reportagens realizada no Google notícias entre 2013 e 2019, utilizando os descritores “Agente Comunitário” e “Tablet”. Elaborou-se um formulário eletrônico no Epi-info 7 com as principais informações de cadauma das notícias, gerando um respectivo banco de dados e clipping das matérias identificadas. Foi feita a análise temporal e geográfica das matérias. A abordagem qualitativa compreendeu a análise dos conteúdos das notícias, com base na análise de conteúdo de Laurence Bardin. Resultados e discussão: Foram encontradas vinte e sete (27) notícias deonze (11) estados de vinte e seis (26) municípios, sendo a maior parte da região Sul e Nordeste do Brasil. Na análise do conteúdo, percebeu-se que o tablet é compreendido como um instrumento que irá fazer com que o trabalho do ACS seja mais dinâmico e prático para eles e para a equipe de saúde da família como um todo, alguns dos termos que mais sedestacaram foram: melhoria, facilidade e agilidade no processo de trabalho. O processo de qualificação dos ACS para o uso da nova tecnologia foi citado em 11 notícias (41%). Conclusão: É importante problematizar a introdução de tablets no processo de trabalho doACS, levantando avanços e desafios, oferecendo também a qualificação apropriada para que estes profissionais possam fazer o melhor uso da ferramenta e não descartar totalmente os papéis, pois podem ser úteis.
Planejamento dos Cuidados Paliativos em Hospital de Retaguarda Clínica através das Linhas de Cuidados
Authors: Ito Süffert Soraya Camargo , Thomaz Raquel Prado , Campos Luciana Silveira , Gomes Eduardo Correa , Ramos Tiago Almeida , Fagundes Marcelo 
Published: March 2022

Introdução: o aumento da expectativa de vida da população brasileira associada a maior prevalência de doenças crônicas não transmissíveis promovem uma maior demanda de recursos assistenciais em saúde, dentre eles os cuidados paliativos (CP). Um serviço hospitalar de Cuidados Paliativos melhora os desfechos em saúde dos pacientes internados e facilita a discussão acerca dos valores dos pacientes, diagnóstico, prognóstico da doença e concordância sobre os objetivos do plano de cuidados, com evidências da redução de indicações de tratamentos não benéficos e até mesmo prejudiciais no final de vida.Metodologia: relato de experiência da implementação das Linhas de Cuidados, para Cuidados Paliativos em hospital de retaguarda clínica e longa permanência de Porto Alegre, utilizando a proposta metodológica de Rosique estruturada em quatro etapas: seleção, desenvolvimento, implementação e avaliação/monitoramento de qualidade. Este processo foi realizado no período de outubro de 2018 a maio de 2019 para organização dos fluxos assistenciais e monitoramento da qualidade.Resultados: obtivemos um planejamento estruturado, com ações estratégicas para implementar os Cuidados Paliativos. Os resultados são descritos nos fluxogramas assistenciais. A construção das LC facilitou a comunicação, coordenação de papéis, organização das atividades da equipe multidisciplinar de atendimento, o adequado registro, monitoramento, avaliação de variações, de resultados, indicadores de desfechos e identificação dos recursos apropriados.Conclusão: este processo qualificou a assistência com a capacitação dos recursos humanos, estabeleceu recursos tecnológicos disponíveis e materiais essenciais, possibilitou o desenvolvimento de protocolos e foi possível instituir indicadores para monitorar a qualidade da assistência aos pacientes e suas famílias.
A preceptoria e o ensino do autocuidado do diabético: das dificuldades aos caminhos possíveis Preceptorship and the teaching of diabetic self-care: from difficulties to possible paths
Authors: Teixeira Patricia Ribeiro da Silva Maia , Almeida Ana Clementina Vieira de , Mourão Lucia Cardoso , Oliveira Gilson Saippa de , Machado Antônia Conceição Cylindro , Penna Fabíola Braz 
Published: March 2022

ResumoObjetivo: discutir as dificuldades e os caminhos possíveis para o ensino do autocuidado do diabético aos alunos em formação na Atenção Básica, evidenciados junto aos profissionais de saúde que atuam como preceptores nos serviços de saúde. Métodos: a pesquisa, tipo estudo de caso com abordagem qualitativa, utilizou como referencial teórico metodológico a análise institucional, em sua abordagem socioclínica institucional. Para a produção de dados foram realizados cinco encontros com trabalhadores da saúde em uma unidade do Programa Médico de Família. Resultados: os resultados revelaram que o sofrimento permeia o contexto do autocuidado, sentido, de um lado, pelos diabéticos, e de outro, pelos profissionais de saúde/preceptores no desempenho do seu duplo papel: educativo e assistencial. Como possíveis caminhos, a pesquisa possibilitou aos profissionais de saúde/preceptores repensarem suas práticas, sugerindo mudanças no processo de trabalho relativo ao ensino do autocuidado ao diabético. Conclusões: destaca-se a necessidade de ampliar as reflexões sobre o papel assistencial e educativo dos profissionais de saúde/preceptores nos serviços do Sistema Único de Saúde.Palavras-chave: Preceptoria; Educação; Assistência à saúde; Diabetes Mellitus. AbstractObjective: to discuss the difficulties and possible ways to teach diabetic self-care to students undergoing training in Primary Care, as evidenced by health professionals who act as preceptors in health services. Methods: the research, a case study with a qualitative approach, used institutional analysis as its theoretical and methodological framework, in its institutional socio-clinical approach. For the production of data, five meetings were held with health workers in a unit of the Family Medical Program. Results: the results revealed that suffering permeates the context of self-care, felt, on the one hand, by diabetics, and on the other, by health professionals/preceptors in the performance of their dual role: educational and assistance. As possible paths, the research enabled health professionals/preceptors to rethink their practices, suggesting changes in the work process related to teaching self-care to diabetics. Conclusions: the need to expand reflections on the care and educational role of health professionals/preceptors in Unified Health System services is highlighted.Keywords: Preceptorship; Education; Health Care; Diabetes Mellitus.
Bioinformatic Investigation of Micro RNA-802 Target Genes, Protein Networks, and Its Potential Prognostic Value in Breast Cancer
Authors: Eini Maryam , Parsi Sepideh , Barati Mahmood , Bahramali Golnaz , Alizadeh Zarei Marziyeh , Kiani Jafar , Azarnezhad Assad , Hosseini Arshad 
Published: March 2022

Background: An increasing number of studies have suggested that unveiling the molecular network of miRNAs may provide novel therapeutic targets or biomarkers. In this study, we investigated the probable molecular functions that are related to microRNA-802 (miR-802) and evaluated its prognostic value in breast cancer utilizing bioinformatics tools.
Methods: PPI network, pathway enrichment and transcription factor analysis were applied to obtain hub genes among overlapping genes of four miRNA target prediction databases. Prognosis value assessments and expression analysis of hub genes using bioinformatics tools, as well as their literature validation were performed.
Results: Our results showed a significant correlation of the miR-802 overexpression with poor patient survival rate (BC, p=2.7e-5). We determined 247 target genes significant for GO and KEGG terms. Analysis of TFs by TRUST showed that RUNX3, FOXO3, and E2F1 are possible TFs that regulate the miR-802 expression and target genes network. According to our analysis; 21 genes might have an important function in miR-802 molecular processes and regulatory networks. The result shows that among these 21 genes, 8 genes (CASC3, ITGA4, AGO3, TARDBP, MED13L, SF1, SNRPE and CRNKL1) are positively correlated with patient survival. Therefore these genes could be considered and experimentally evaluated as a prognostic biomarker for breast cancer.
Conclusion: The comprehensive bioinformatics study on miR-802 target genes provided insight into miR-802 mediated pathways and processes. Furthermore, representing candidate target genes by prognostic values indicates the potential clinical application of miR-802 in breast cancer.
Designing a Multi-Epitope Antigen for Serodiagnosis of Strongyloides stercoralis Based on L3Nie.01 and IgG Immunoreactive Epitopes
Authors: Movahedpour Ahmad , Mostafavi-Pour Zohreh , Sarkari Bahador , Taheri-Anganeh Mortaza , Nezafat Navid , Savardashtaki Amir , Ghasemi Younes 
Published: March 2022

Background: Serological diagnosis of Strongyloides stercoralis (S. stercoralis) is frequently challenging because of cross-reactivity with other parasitic nematodes. Therefore, it is necessary to introduce novel serological tests with high performance to properly diagnose this neglected parasitic infection. The purpose of the current study was to design a multi-epitope construct for the diagnosis of S. stercoralis.
Methods: For the purpose of this study, first, highly antigenic segments and potential immunodominant epitopes of S. stercoralis were identified from two antigenic proteins, and then all of the selected parts were linked by an appropriate linker. Next, the physico-chemical features of the designed construct were analyzed. Then, tertiary structures of the construct were built and evaluated to find out the best one. Lastly, the amino acid sequence was reverse-translated and optimized for over-expression in Escherchia coli (E. coli).
Results: The bioinformatic evaluation indicated that the designed protein construct could be hydrophilic, thermostable, and acidic and the estimated half-life was more than 10 hr in E. coli.
Conclusion: According to the results of the study, the designed construct could be used as an efficient antigen in the ELISA system for serological diagnosis of human strongyloidiasis.
Could Frequency of Candida spp. Be Different on Various Parts of Tongue in Denture and Non-denture Wearers?
Authors: Owlia Fatemeh , Hekmatimoghaddam Seyedhossein , Kheirollahi Faraz 
Published: March 2022

Background & Objectives: Oral Candidiasis is the most common opportunistic fungal infection that could affect the oral mucosa. Studies to date did not compare the colony count of candida of the anterior and posterior surfaces of the tongue. This study makes an attempt to compare the Candida spp. on the anterior and posterior surfaces of tongue among healthy denture and non-denture wearers.
Materials & Methods: Participants of the current cross-sectional study were 26 healthy denture wearers (DW) and 10 healthy non-denture wearers (NDW). Oral specimens were collected from anterior and posterior tongue dorsa by swabbing for mycological examination. After 48 h incubation on Sabouraud dextrose agar (S) and chloramphenicol (SC) and chloramphenicol and Cycloheximide (SCC) ™ medium, C. Albicans and non-Albicans were identified by Germ Tube test. Isolated colonies were evaluated. Obtained data were analyzed by SPSS software version 17. Chi-square, Mann-Whitney and Wilcoxon tests were used for statistical comparison of data due to non-compliance with normal distribution.
Results: The higher rate of isolated colonies was seen in DW group compared to NDW group (P-Value = 0.03). There was a significant difference between candida colonization of anterior and posterior surface of tongue (P-Value = 0.006). C. Albicans was the most common isolated candida species.
Conclusions: Mycological findings of this study revealed that the presence of denture can increase colonization of candida on the posterior surface of the tongue. It could be an important role in choosing the best form for medical management of oral candidiasis.
Toxicity Effects of Lipid Nanoparticles Containing Artemisia Absinthium L Essential Oil on BRC-03 Breast Cancer Cell Line
Authors: Teimouri Maryam , Pooladi Mehdi 
Published: March 2022

Background & Objective: According to research, breast cancer is the second most common cancer among women. Nanomaterials of various materials significantly increase the solubility, stability and effective drug delivery, and in recent years research on the effectiveness of essential oils and plant extracts in inhibiting the growth of cancer cells is expanding. Artemisia belongs to the Asteraceae family. In many studies, the effectiveness of the essential oil and extract of this plant in inhibiting the growth of cancer cells has been reported.
Materials & Methods: In this experiment, nanoparticles containing Artemisia absinthium L essential oil (thistle) were first synthesized by homogenizer & sonication method and then their physicochemical properties were determined, such as particle size, particle size distribution, zeta potential, percentage of loading efficiency. Then, the anti-apoptotic effect of lipid nanoparticles containing Artemisia essential oil in breast cancer cells (BRC-03) was evaluated.
Results: The results of cellular effect of lipid nanoparticles containing essential oil of Artemisia absinthium L (thistle) on breast cancer cells showed that increasing the concentration of lipid nanoparticles containing essential oil of this plant reduced the survival rate of cancer cells. Breast (BRC-03) was purified compared to essential oil (p <0.05).
Conclusion: Nano-essential oil of this plant is effective in reducing the IC50 of the drug and in increasing the cytotoxicity of the essential oil of the plant. Lipid nanoparticles containing essential oil of Artemisia absinthium L (thistle) activated the apoptotic pathway in breast cancer cells.
Evaluation of the Long noncoding RNA NONHSAT037832 Gene Expression Changes in Patients with Thyroid Carcinoma
Authors: Keysan Parya , Ghorbian Saeid 
Published: March 2022

Background & Objective: Thyroid cancer is the most common endocrine disorder in the world. One of the main causes of this cancer is genetic changes, in particular mutations in the tumor and oncogene inhibition genes. In addition to mutations, some non-encoding RNAs called lncRNAs play an important role in the progression of tumors. NONHSAT is a lancer and has recently been referred to the role of this gene in various types of cancer. This study aimed to evaluate the lncRNA-NONHSAT037832 gene expression changes in patients with thyroid carcinoma.
Materials & Methods: A cross-sectional study was conducted on 140 homogeneous samples (70 cancer tissue homogeneous samples and 70 homogeneous healthy tissue samples) from patients with thyroid cancer referred to Alzahra Hospital in Tabriz. Using the Real-Time PCR, changes in the expression of the lncRNA-NONHSAT037832 gene were evaluated. In addition, the relationship between lncRNA-NONHSAT037832 gene expression changes and clinical parameters was investigated.
Results: The results showed that the expression of lncRNA-NONHSAT037832 gene expression in the tissues of cancer was significantly higher than healthy tissues (P <0.001) and a significant relationship with MTN (P = 0.001) and node metastasis Lymphoma was shown (P = 0.30).
Conclusion: According to the results, the lncRNA-NONHSAT037832 gene may be used as a prognostic biomarker for thyroid cancer.
The Effect of Simvastatin and Vitamin D Co-Administration on Rats Brain Function and Behavior: A Behavioral and Biochemical Study
Authors: Ranaiy Mohammad Saleh , Farokhi Farah , Babaei Balderlou Farrin 
Published: March 2022

Background & Objective: The simvastatin (Sim) is a lipophilic statin and can cross the blood-brain barrier. The role of vitamin D (Vit D) in brain development and function has been supported over the past decade. This study aimed to evaluate the effect of simvastatin on memory and anxiety levels in healthy male rats.
Materials & Methods: In this experimental study, 36 male Wistar rats weighing 250-300 g were randomly divided into six groups (n=6) including control, Vit D (5 µg/kg/day; IP), Simvastatin (1 mg/kg/day; orally) (SimL), Simvastatin (10 mg/kg/day; orally) (SimH), SimL + Vit D and SimH + Vit D. After 28 days, at the end of the treatment, the behavioral anxiety test and memory behavioral test were performed. Then, the rats were euthanized, and oxidative stress markers of the brain, serum low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and cholesterol levels were investigated. Data were analyzed by SPSS 24 software and Tukey’s test.
Results: Co-administration of simvastatin and vitamin D significantly increased working memory, catalase activity, total antioxidant capacity, HDL-C, and decreased anxiety levels, malondialdehyde (MDA) concentration, cholesterol, and LDL-C compared to the control group (p<0.05). However, the administration of simvastatin and vitamin D alone did not significantly change the mentioned parameters compared to the control group (p>0.05).
Conclusion: Co-administration of simvastatin and vitamin D can improve brain function by reducing oxidative stress and cholesterol levels.
Effects of Melatonin on Hepatic and Renal Complication of Diabetes
Authors: Safarzadeh Hamed , Zargari Felor 
Published: March 2022

Background & Objective: Diabetes is associated with biochemical and pathological changes in liver and kidney tissue. One of the most important mechanisms in causing complications of diabetes is increasing the production of free radicals due to impaired glucose metabolism. Some studies have shown that melatonin is effective in protecting tissues due to its antioxidant role. The aim of this study was to evaluate the effect of melatonin on biochemical factors, histopathological changes of liver and kidney in streptozotocin-induced (STZ) diabetic rats.
Materials & Methods: In this experimental study, 40 adult male rats were randomly divided into 4 groups (n=10): control group, diabetic group, melatonin group (20mg/kg BW) and melatonin + diabetic group. At the end of the 6th week, blood samples were collected. Biomarkers of liver function (alkaline phosphatase: ALP, aspartate aminotransferase: AST, alanine aminoteransferase: ALT) and kidney biomarkers (urea, uric acid, BUN, creatinine), serum glucose and histopathological changes of these tissues were evaluated. Data analysis was performed using SPSS and the significance level was P<0.05.
Results: Melatonin treatment significantly (P<0.05) reduced hyperglycemia, hepatic enzymes and renal biochemical factors due to diabetes and improved histopathological changes of liver and kidney tissue.
Conclusions: Melatonin consumption can be effective in improving the renal and kidney complications of diabetes.