bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic and environmental perturbations alter the rhythmic expression pattern of a circadian long non-coding RNA, Per2AS, in mouse liver
Authors: Miao Lin , Batty Kyle R. , Jackson Ayana N. , Pieno Heather A. , Rhoades Maisy W. , Kojima Shihoko 
Published: October 2022

Background: Long non-coding RNAs (lncRNAs) play a wide variety of biological roles without encoding a protein. Although the functions of many lncRNAs have been uncovered in recent years, the regulatory mechanism of lncRNA expression is still poorly understood despite that the expression patterns of lncRNAs are much more specific compared to mRNAs. Here, we investigated the rhythmic expression of Per2AS, a novel lncRNA that regulates circadian rhythms. Given that Per2AS expression is antiphasic to Period2 (Per2), a core circadian clock gene, and transcribed from the antisense strand of Per2, we hypothesized that the rhythmic Per2AS expression is driven either by its own promoter or by the rhythmic Per2 transcription via transcriptional interference. Methods: We leveraged existing circadian RNA-seq datasets and analyzed the expression patterns of Per2AS and Per2 in response to the genetic or environmental disruption of the circadian rhythm in mouse liver. We tested our hypotheses by comparing the changes in the expression patterns of Per2AS and Per2. Conclusions: We found that, in some cases, Per2AS expression is independently controlled by other circadian transcription factors. In other cases, the pattern of expression change is consistent with both transcriptional interference and independent regulation hypotheses. Although additional experiments will be necessary to distinguish these possibilities, findings from this work contribute to a deeper understanding of the mechanism of how the expression of lncRNA is regulated.
Management of penetrating chest trauma in the context of major trauma networks
Authors: Stretch Benjamin , Kyle Amy , Patel Mihir 
Published: October 2022

Major trauma networks reduce mortality in critically injured patients. Trauma patients should be appropriately triaged straight from the scene of injury, avoiding secondary transfer from a trauma unit. Selection criteria in regionally agreed triage tools are designed to identify which patients should be taken directly to the major trauma centre. Patients with life-threatening injuries still arrive at ‘trauma units’ in circumstances such as self-presentation, under triage, physiological instability or long journey time to the major trauma centre. This article presents a theoretical case of a haemodynamically unstable patient with penetrating injuries, and discusses the management of chest trauma, including diagnosis of life-threatening injuries, resuscitation strategies and definitive surgical management. Secondary transfer to the major trauma centre should be considered after instituting the minimal life-saving interventions. What constitutes a life-saving intervention requires an individual dynamic risk assessment and an understanding of major trauma networks.
Critical limb ischaemia in the time of COVID-19: establishing ambulatory service provision
Authors: Chana M , Muse S , Ball S , Bennett R , McCarthy R 
Published: October 2022

Introduction The coronavirus disease 2019 (COVID-19) pandemic enforced changes to healthcare services at a pace and extent not seen previously in the NHS. The Royal Devon and Exeter provides regional vascular surgery services. A consultant-led urgent ‘hot clinic’ was established, providing patients with ambulatory care. We aim to describe the service for critical limb ischaemia (CLI) before and during the COVID-19 pandemic, and evaluate this against recommended best practice. Methods Retrospective review of electronic databases and records of patients with CLI during a non-COVID vs COVID-19 period. Primary outcome measures were those established by guidance from the Vascular Society of Great Britain and Ireland. Results Non-COVID vs COVID-19: total patients n=97 vs 96, of which CLI patients n=29 vs 21. Median length of stay 15 vs 0 days (p<0.001); median time from referral to specialist review 0 vs 3 days (p<0.001); multidisciplinary team meeting (MDT) recorded 3% vs 29%; median time to intervention 6 vs 8 days; conservative management 52% vs 67%; endovascular 28% vs 10%; open surgery 21% vs 24%; 30-day survival 79% vs 76%. Conclusion COVID-19 imposed a major change to the service for patients with CLI with a focus on ambulatory care pathways for diagnosis and intervention. We observe a significant reduction in overall length of stay with no clinically significant change in time to consultant review, time to imaging, overall management strategy or outcomes. The results of this study show that patients with CLI can be managed safely and effectively on an ambulatory basis in accordance with established best practice.
Transmission optimized LWIR metalens
Authors: Nalbant Halil Can , Balli Fatih , Yelboğa Tolga , Eren Arda , Sözak Ahmet 
Published: October 2022

Thermal imaging at the infrared wavelength regime has long been applied to different areas such as agriculture and defense industries. Metasurfaces, 2D engineered ultra-thin structures, have attracted much attention due to their compact size, superior performance, and different functionalities at optical frequencies. This work details the design and fabrication of high transmission metalenses operating at the long-wave infrared (LWIR) spectrum. We minimize the reflection losses through anti-reflection coating (ARC) while maintaining the full wavefront control at the central wavelength 9.07 µm. Our unit cell structure provides an average transmission of 97.5%. We experimentally verify our results and show that the fabricated metalenses perform diffraction-limited imaging at the design wavelength.
Linear-cavity Er-doped fiber mode-locked laser with large wavelength tunability
Authors: Jang Yoon-Soo , Park Jungjae , Jin Jonghan 
Published: October 2022

A linear-type wavelength-tunable all-polarization-maintaining fiber mode-locked laser is proposed for the first time, to our knowledge, and is implemented with an Er-doped fiber and polarization-maintaining fiber components. The tuning range of the center wavelength is from 1533.7 nm to 1565.6 nm. The linear-type configuration makes the proposed laser simpler and more compact, allowing it to achieve the highest repetition rate of 126.5 MHz among C-band wavelength-tunable mode-locked lasers due to its short cavity length. Also, its polarization-maintaining fiber components provide reliable operating robustness. The significant wavelength tunability and high repetition rate of the proposed laser can be expected to make it an attractive resource for various applications, including optical communications, broadband spectroscopic LIDAR, and high-precision ranging.
Expertise in die Fläche bringen: Analyse der Covid-19-Telekonsile und szenariobasierte Handlungsempfehlungen
Authors: Neft Florian , Kappler Karolin Eva , Dohmen Sandra Maria , Juhra Christian , Sperling Kathrin , Bause Daniela , Smolnik Stefan 
Published: October 2022

ZusammenfassungDas Auftreten der unbekannten Krankheit Covid-19 und die steigenden Covid-19-Fallzahlen stellten das Gesundheitswesen vor Herausforderungen. Die Häuser der Allgemeinversorgung waren bis dato größtenteils nicht mit der Behandlung eines schweren Lungenversagens vertraut und so musste dieses Wissen aus den Expertenzentren in die Häuser der Allgemeinversorgung transferiert werden. Dazu wurden im Rahmen der Vorstufe des Virtuellen Krankenhauses Nordrhein-Westfalen (VKh.NRW) Telekonsile genutzt. Sie finden zwischen zwei Ärzten der Intensivmedizin statt, stellen ortsunabhängig Expertenwissen bereit und erhöhen so die Behandlungsqualität sowie -effizienz. In der Evaluation dieser Telekonsile zeigen sich unterschiedliche Nutzungsszenarien. Während zu Beginn größtenteils allgemeine Diagnose- und Therapiewege von Interesse waren, kamen später detailliertere Fragen auf, für die unter anderem ein Pharmakologe notwendig war. Dieser Anwendungsfall bedarf mehr explizites Wissen, Patienteninformationen und damit erhöhte Technologieanforderungen. Im Rahmen der technischen Infrastruktur zeigen sich allerdings Barrieren. Daher wird evaluiert, welches Wissen in den unterschiedlichen Anwendungsfällen der Telekonsile ausgetauscht wird und welche technologischen Voraussetzungen erfüllt sein müssen, damit Telekonsile stärker zur Verwendung kommen und das Gesundheitswesen gleichermaßen entlasten und verbessern.
Patients’ knowledge about medicines improves when provided with written compared to verbal information in their native language
Authors: T. Rameshkumar , I. U. Haputhanthrige , M. Y. Misbahunnisa , P. Galappatthy 
Published: October 2022

Background
Patient’s knowledge on the medicines prescribed is vital in reducing medication errors and improving adherence and patient satisfaction. This study assessed the patients’ knowledge on medicines prescribed and compared improvement following verbal or written information given in patients own language.

Methods
Seventy-five trainee pharmacists randomised to verbal or written groups, provided information to consecutive consenting patients attending medical and surgical clinics in the outpatient department of National Hospital of Sri Lanka. Baseline knowledge and immediate recall knowledge was scored on 5 categories (name, indication, dose, frequency of administration, and additional relevant details) for each drug prescribed. Scores <5/10, 5-7/10 and > 7/10 were considered as poor, moderate, and good knowledge. Sample of 384 in each group had 80% power to detect a 10% difference in knowledge level. Data was analysed using SPSS v26.

Results
Of the 747 patients, 429(57%) were females, mean age was 54.3 years(SD±12), 450(60%) were on 5 or more drugs and 149(20%) were elderly (>65 years). At baseline, knowledge scores were comparable in both groups with 47% (352/747) patients having poor knowledge, 34% (254/747) moderate knowledge and only 19% (141/747) good knowledge. Patients knew the name only on 1653(42%) drugs prescribed, indication on 1603(41%), dose on 860(22%), administration details on 2255(58%) and additional details (adverse effects/storage) only on 267(7%) medicines prescribed. After the intervention, 276(74%) patients had good knowledge (score>7), in written group vs 217(58%) in verbal group (p<0.05). In all 5 aspects knowledge improved significantly more in written group compared to the verbal group. Binomial logistic regression analysis indicated that ordinary level (OR:2.32), advanced level(OR:2.13) and university education(OR:3.72), and lower number of medicines prescribed(OR:0.83) significantly increased the odds of having a “Good Knowledge on Medicines”.

Conclusion
Patient’s knowledge on medicines prescribed was poor and providing the medication plan in writing in patients’ own language would improve the knowledge significantly compared to providing verbal information.
Prevalence of corneal findings and their interrelation with hematological findings in monoclonal gammopathy
Authors: Al Hariri Mohammad , Munder Markus , Lisch Walter , Schuster Alexander K. , Fehr Eva-Marie , Jacobi Björn , Desuki Alexander , Kreft Andreas , Gericke Adrian , Pfeiffer Norbert , Wasielica-Poslednik Joanna 
Published: October 2022

Purpose
To determine prevalence of paraproteinemic keratopathy (PPK) among patients with monoclonal gammopathy (MG). To evaluate interrelation between corneal and hematological parameters in patients with PPK.

Methods
Fifty-one patients with monoclonal gammopathy of undetermined significance (n = 19), smoldering multiple myeloma (n = 5) or multiple myeloma (n = 27) were prospectively included in this study. Best-corrected visual acuity, slit-lamp biomicroscopy, Scheimpflug tomography, in-vivo confocal laser scanning microscopy, optical coherence tomography and complete hematological workup were assessed.

Results
We identified n = 19 patients with bilateral corneal opacities compatible with PPK. PPK was newly diagnosed in 13 (29%) of 45 patients with a primary hematological diagnosis and in n = 6 patients without previous hematological diagnosis. The most common form was a discreet stromal flake-like PPK (n = 14 of 19). The median level of M-protein (p = 0.59), IgA (p = 0.53), IgG (p = 0.79) and IgM (p = 0.59) did not differ significantly between the patients with and without PPK. The median level of the FLC κ in serum of patients with kappa-restricted plasma cell dyscrasia was 209 mg/l in patients with PPK compared to 38.1 mg/l in patients without PPK (p = 0.18). Median level of FLC lambda in serum of patients with lambda-restricted plasma cell dyscrasia was lower in patients with PPK compared to patients without PPK (p = 0.02).

Conclusion
The PPK was mostly discreet, but its prevalence (29%) was higher than expected. Median level of the monoclonal paraprotein was not significantly higher in patients with PPK compared to patients without PPK. Our results suggest a lack of correlation between morphology and severity of the ocular findings and severity of the monoclonal gammopathy.

Trial registration
German Clinical Trial Register: DRKS00023893.
Rural-urban differences in the initiation of oral anticoagulant therapy in patients with incident atrial fibrillation: A Finnish nationwide cohort study
Authors: Teppo Konsta , Jaakkola Jussi , Langén Ville L. , Biancari Fausto , Halminen Olli , Linna Miika , Haukka Jari , Putaala Jukka , Mustonen Pirjo , Kinnunen Janne , Luojus Alex , Hartikainen Juha , Airaksinen K. E. Juhani , Lehto Mika 
Published: October 2022

Aims
Little is known about rural-urban differences in the treatment and outcomes in patients with atrial fibrillation (AF). We aimed to assess whether the initiation of oral anticoagulant (OAC) therapy in patients with AF differs between those with rural and urban residence.

Methods
The registry-based FinACAF cohort covers all patients with AF from all levels of care in Finland. Patients were divided into rural and urban categories and into urbanization degree tertiles based on their municipality of residence at the time of AF diagnosis. The outcome was the first redeemed OAC prescription.

Results
We identified 222 419 patients (50.1% female; mean age 72.8 (SD 13.2) years) with incident AF during 2007–2018. Urban residence was associated with a lower rate of OAC therapy initiation (adjusted subdistribution hazard ratio (SHR) (95% CI) 0.96 (0.95–0.97)). Correspondingly, an inverse graded dose-response relationship was observed between higher urbanization degree tertile and OAC initiation rate (highest tertile compared to lowest: adjusted SHR (95% CI) 0.94 (0.93–0.95)). The adoption of direct oral anticoagulants for stroke prevention was faster among patients with urban residence.

Conclusion
This nationwide cohort study documented that urban residence is associated with a slightly lower rate of OAC therapy initiation in patients with incident AF, but faster adoption of direct oral anticoagulant use.
A cross-sectional analysis of podiatrist-initiated review processes after issuing prescribed foot orthoses
Authors: Donnan Luke , Horn Anna , Baker Emma 
Published: October 2022

Background
Foot orthoses are widely used in clinical practice to treat foot, lower limb and back pathology. As published information guiding the clinical use of foot orthoses is scarce, the aim of this study is to profile the review processes used by practicing podiatrists after issuing an orthotic device.

Methods
A cross-sectional observational study design formed the basis for a self-administered online questionnaire. The questionnaire was distributed through podiatry networks based in Australia.

Results
Two-hundred and thirty-eight practicing podiatrists participated in this study. Ninety-seven percent of respondents indicated that they would recommend a review appointment after the initial fitting of an orthotic device. Forty percent (n = 84) of respondents scheduled the first review appointment four weeks after the initial fitting, while 33% (n = 69) preferred a two-week review period. A second review consultation was standard practice for 32% (n = 68) or respondents, and were typically scheduled either two (23%, n = 12) or four (38%, n = 20) weeks after the initial review consultation. Annual review of orthotic devices was recommended by 64% (n = 123) of participants in the study, while 19% (n = 37) would suggest that yearly reviews were scheduled only if required.

Conclusions
Variation was identified in the orthotic review processes used by practicing podiatrists, although most respondents recommend a routine short-term review appointment for foot orthoses. It is not clear why practitioners adopt such varied approaches. In the absence of any clear evidence on this topic, it may be that the differing approaches to patient review reflect different philosophical perspectives regarding patient management.
The effects of a 5-year physical activity on prescription (PAP) intervention in patients with metabolic risk factors
Authors: Lundqvist Stefan , Cider Åsa , Larsson Maria E. H. , Hagberg Lars , Björk Marcus Praetorius , Börjesson Mats 
Published: October 2022

Background
Increased physical activity (PA) has positive effects on health and longevity. In Swedish healthcare, the physical activity on prescription (PAP) method reportedly increases patients’ PA levels for up to 12 months, but long-term follow ups are lacking. As it remains difficult to maintain lifestyle changes, our aim was to evaluate adherence and clinical effects at a 5-year follow-up of PAP treatment in primary healthcare.

Methods
This longitudinal, prospective cohort study included 444 patients, (56% female), aged 27–85 years, with at least one metabolic risk factor. Participants were offered PAP by nurses or physiotherapists. The PAP intervention included an individualised dialogue, a PA recommendation by written prescription, and individually adjusted follow-up over 5 years, according to the Swedish PAP model. Patient PA level, metabolic risk factors, and health related quality of life (HRQoL) were measured at baseline and at the 6-month, 1.5-year, 2.5-year, 3.5-year, and 5-year follow-ups. Estimated latent growth curves were used to examine levels and rates of change in the outcomes.

Results
The study dropout rate was 52%, with 215 of 444 patients completing the 5-year follow-up. At follow-up, the mean PA level had increased by 730 MET-minutes per week or 3 hours of moderate-intensity PA/week when compared to baseline. During the 5-year intervention, we observed significant positive changes (p ≤ 0.05) in 9 of 11 metabolic risk factors and HRQoL parameters: body mass index, waist circumference, systolic and diastolic blood pressure, fasting plasma glucose, triglycerides, cholesterol, high-density lipoprotein, and mental component summary.

Conclusion
This first evaluation of a 5-year PAP intervention in primary care demonstrated positive long-term (5 years) effects regarding PA level, metabolic health, and HRQoL. The recorded long-term adherence was ~50%, which is in line with medical treatment. Despite limitations, PAP can have long-term effects in an ordinary primary care setting.
Physical activity, fear avoidance beliefs and level of disability in a multi-ethnic female population with chronic low back pain in Suriname: A population-based study
Authors: Ho-A-Tham Nancy , Struyf Niels , Ting-A-Kee Beverly , de Almeida Mello Johanna , Vanlandewijck Yves , Dankaerts Wim 
Published: October 2022

Background
Chronic low back pain (CLBP) is an important cause for reduced daily physical activity (PA) and loss of quality of life, especially in women. In Suriname, a middle-income country in South America, the relationship between PA and CLBP is still unknown.

Aims
To assess the level of PA in women with CLBP of different ethnicity, and to identify whether fear avoidance beliefs (FAB), disability, co-occurring musculoskeletal pain sites and various sociodemographic and lifestyle factors were associated with self-reported PA.

Methods
A cross-sectional community-based house-to-house survey was conducted between April 2016 and July 2017. The survey followed the Community Oriented Program for Control of Rheumatic Diseases methodology. Selection criteria were being female of Asian-Surinamese, African-Surinamese or of Mixed ethnicity and aged 18 or older, living in an urban area, and reporting CLBP. Data was collected on PA, FAB, disability, co-occurring musculoskeletal pain sites, CLBP intensity and sociodemographic and lifestyle factors.

Results
Urban adult women with current CLBP (N = 210) were selected. Nearly 57% of the population met the WHO recommendation on PA, with work-related PA as the largest contributor to total self-reported PA. Most women showed low FAB scores (FABQ-Work ≤34 (96.2%) and FABQ-PA ≤14 (57.6%)) and low disability levels (Oswestry Disability Index ≤20 (62.4%)). An inverse association between total PA and FABQ-Work (OR = 0.132, CI: 0.023; 0.750) was found. In contrast, total PA had a significant, positive association with disability (OR = 2.154, CI: 1.044; 4.447) and workload (OR = 2.224, CI: 1.561; 3.167). All other variables showed no association with total PA.

Conclusion
This was the first study in Suriname reporting that 43.3% of urban adult women with CLBP were physically inactive. Total self-reported PA is influenced by FABQ-Work, average to heavy workload and moderate to severe disability. In this study, PA-Work was the major contributor to total PA. Therefore, future longitudinal studies should evaluate different types and aspects of PA in relation to CLBP management.
Effect of active tuberculosis on the survival of HIV-infected adult patients who initiated antiretroviral therapy at public hospitals of Eastern Ethiopia: A retrospective cohort study
Authors: Sime Tadesse , Oljira Lemessa , Diriba Aboma , Firdisa Gamachis , Gezimu Wubishet 
Published: October 2022

Background
In resource-limited countries such as Ethiopia, tuberculosis is the major cause of morbidity and mortality among people living with the human immunodeficiency virus. In the era of antiretroviral therapy, the effect of tuberculosis on the survival of patients who are living with human immunodeficiency virus has been poorly understood in Ethiopia. Therefore, this study aimed to determine the effect of active tuberculosis on the survival of HIV-infected adult patients who initiated antiretroviral therapy in public hospitals in Eastern Ethiopia.

Methods
An institution-based retrospective cohort study was conducted among 566 participants from January 1, 2014, to June 30, 2018. The collected data were entered into EpiData version 3.1 before being exported to Stata version 14 for analysis. A Cox proportional hazard model was used to determine the effect of active tuberculosis on the survival of HIV-infected adult patients who initiated antiretroviral therapy, and a p-value less than 0.05 and a 95% confidence level were used to declare statistical significance.

Result
Of the 566 patients included in the study, 76 died. The mortality rate was 11.04 per 100 person-years in tuberculosis co-infected patients, while it was 2.52 per 100 person-years in non-tuberculosis co-infected patients. The patients with tuberculosis co-infection had a 2.19 times higher hazard of death (AHR: 2.19; 95% CI: 1.17, 4.12) compared to those without tuberculosis. Advanced clinical stage, low CD4+ cell count, and previous episodes of an opportunistic infection other than tuberculosis were found to be independent predictors of mortality.

Conclusion
Co-infection with tuberculosis at antiretroviral therapy initiation increases the hazard of death approximately two-fold. Hence, we recommend key organizations to enhance the region’s collaborative interventional and preventative strategies for TB and HIV.