bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel STING-targeted PET radiotracer for alert and therapeutic evaluation of acute lung injury
Authors: Xu Duo , Jiang Zebo , Zhu Tianxing , Wang Fen , Xiao Yitai , Liang Zibin , Bi Lei , Huang Guolong , Shan Hong , Pang Pengfei , Li Dan 
Published: April 2022

Abstract
Acute lung injury (ALI), as a common clinical emergency, is pulmonary edema and diffuse lung infiltration caused by inflammation. The lack of non-invasive alert strategy, resulting in failure to carry out preventive treatment, means high mortality and poor prognosis. Stimulator of interferon genes (STING) is a key molecular biomarker of innate immunity in response to inflammation, but there is still a lack of STING-targeted strategy. In this study, the first STING-targeted PET tracer, [18F]FBTA, was labeled with high radiochemical yield and molar activity. We confirmed that [18F]FBTA has a strong STING binding affinity and can be used for PET imaging in ALI mice to alert early lung inflammation and to assess the efficacy of drug therapy. Our STING-targeted strategy also reveals that [18F]FBTA can trace ALI before reaching the computed tomography (CT) diagnostic criteria, and demonstrates its better specificity and distribution than [18F]fluorodeoxyglucose ([18F]FDG).
POLDIP3 facilitates the activation and maintenance of DNA damage checkpoint in response to replication stress
Authors: Zhang Sufang , Lee Ernest Y. C. , Lee Marietta Y. W. T. , Zhang Dong 
Published: April 2022

Abstract
The replication stress response is crucial for the maintenance of a stable genome. POLDIP3 was initially identified as one of the DNA polymerase δ (Pol δ) interacting proteins almost twenty years ago. Using a variety of in vitro biochemical assays, we previously established that POLDIP3 is a key regulator of the enzymatic activity of Pol δ. However, the in vivo function of POLDIP3 has been elusive for a long time. Here, we first generated POLDIP3 knockout (KO) cells using the CRISPR technology and investigated its biological functions in vivo. We showed that though the POLDIP3-KO cells manifest no pronounced defect in the global DNA synthesis under non-stress conditions, they are sensitive to a variety of replication fork blockers. Intriguingly, we found that POLDIP3 plays a crucial role in the activation and maintenance of the DNA damage checkpoint in response to exogenous as well as endogenous replication stress. Our results thus indicate that when the DNA replication fork is blocked, POLDIP3 can be recruited to the stalled replication fork and functions to bridge the early DNA damage checkpoint response and the later replication fork repair/restart.
Coping strategies used by Parents of children with chronic kidney conditions: A Qualitative Study
Authors: Parizad Naser , MaslakPak Masoumeh Hemmati , Feizi Aram , Khorsandi Fatemeh 
Published: April 2022

Abstract
Background: The chronic condition of children negatively affects them and their whole family, causing various psychosocial stresses. Meanwhile, parents use coping strategies to reduce these stresses and adapt to the child's health condition. Therefore, the present study aimed to explain the strategies employed by parents of children with chronic kidney conditions to cope with the child's condition.Methods: This qualitative study was conducted in Shahid Motahari Hospital, Urmia, Iran. In this study a total of 22 parents of children with chronic kidney conditions were recruited using purposive sampling. Data were collected using semi-structured face-to-face interviews, and the concepts were then extracted using content analysis.Results: The theme entitled "Striving for stability" was extracted from the results and consisted of two sub-themes of "Self-management" and "Situation management". The sub-theme of "Self-management" included concepts of "Seeking solace through inculcation" and "Taking refuge in spirituality" and the sub-theme of "Situation management" included concepts of "Striving to learn", "Determination to continue treatment", and "Striving to solve the problem".Conclusion: Awareness of parents' experiences of caring for a child with a chronic kidney condition is of critical importance in meeting the unmet needs of children and their parents and can serve as a guide for other parents. This knowledge can also be important in designing community-based interventions, where parents play a key role in implementing and evaluating the intervention.
Implementation of advance care planning decision aids for patients undergoing high-risk surgery: A field-testing study
Authors: YAMAMOTO KANAKO , Kaido Toshimi , Yokoi Tadao , Shimada Gen , Takeda Takashi , Nakayama Kazuhiro 
Published: April 2022

Abstract
Background: Patients undergoing high-risk surgery are at risk of sudden changes in worsening. Advance care planning (ACP) support for patients who want active treatment is insufficient in setting outcomes, as it only targets better end-of-life care. Therefore, it is necessary to clarify how ACP support is implemented in the field and its effects. This study aimed to assess whether the development of two patient decision aids (PtDAs) can support preoperative ACP in patients undergoing high-risk surgeries.Methods: This field-testing implemented two PtDAs that met the international criteria developed by the researchers to patients before surgery. The study participants included patients who were scheduled to be admitted to the intensive care unit after surgery at one acute care hospital in Japan and their families. The primary outcome was decision satisfaction evaluated by the SURE test, and the secondary outcomes were perception of the need to discuss ACP before surgery and mental health status. Data were collected before (preoperative outpatients, baseline) and after providing PtDAs (in the hospital) and after discharge. Results were analyzed using a mixed-methods approach.Results: Nine patients were enrolled, of whom seven and their families agreed to participate. The SURE test result after discharge was four points (indicating no decisional conflict) for patients who were able to discuss with the researcher, patient, and family before discharge. Assisting patients with ACP before undergoing a high-risk surgery allowed them to recognize in advance the need for ACP, have an opportunity to share their thoughts with family members, and begin planning their own lives. Patients and their families were prepared to promote shared decision-making with healthcare providers and implement ideas into action.Conclusions: Two PtDAs supporting ACP in patients undergoing a high-risk surgery were developed, evaluated, and accepted; however, they did not involve any discussion of patients’ ACP treatment wishes with their families. Medical personnel should be coached to connect with adequate support. PtDAs for ACP before surgery could promote shared decision-making among patients, families, and healthcare providers.
Chemical composition of Rhododendron luteum essential oil and its larvacidal effectiveness on Diprion pini larvae
Authors: GOKTURK Temel , BURJANADZE Medea 
Published: April 2022

Abstract
Diprion pini is known as a significant forest pest that damages pine needles in many countries of the world today. Due to the risk of chemical contamination of synthetic pesticides applied against insects, essential oil use is becoming more popular. In this research, the chemical composition of Rhododendron luteum essential oil was analyzed by using GC-MS, and its larvicidal effects on six D. pini larval stages were investigated. The research was conducted in the Artvin Coruh University Central Laboratory under laboratory conditions settings 25±1ºC temperature, 65±5% relative humidity, and a photoperiod of 14:10 h (L:D) in 2019-2020. β-caryophyllene (15,39%), benzyl benzoate (11,05%), δ-cadinene (7,06%), benzyl salicylate (6,08%), and α-pinene (4,14%) were found as the primary components of R. luteum oil. It is observed that the applications of R. luteum essential oil at the doses of 10, 15, and 20 μL/Petri and the controls at 24h, 48h, 72h and 96h induced fatalities at varied rates (20,1-100 %) on six D. pini larval stages (L1, L2, L3, L4, L5, L6). The highest toxicity was identified at L1 (0,72 μL/larva) and the lowest toxicity was determined at L6 (11,92 μL/larva) according to LD50 and LD90 values. To sum it up, R.luteum essential oil showed high toxicity against L1 and L2 larval stages of D. pini.
Why do Chinese older adults in Hong Kong delay or refuse COVID-19 vaccination? A qualitative study based on Grounded Theory
Authors: YUAN Jiehu , Lam Wendy Wing Tak , Xiao Jingyi , Ni Michael Yuxuan , Cowling Benjamin John , Liao Qiuyan 
Published: April 2022

Abstract

Background
Older adults have been disproportionately affected by the COVID-19 pandemic. While COVID-19 vaccines are effective for reducing mortality and severe complications, vaccine hesitancy remains a substantial concern particularly among older adults.
Objective
This was a qualitative study to explore the psychological and contextual factors that contribute to Chinese older adults’ delay or refusal of COVID-19 vaccines.
Methods
Semi-structured in-depth interviews were conducted with 27 older adults aged ≥ 60 years who had never received COVID-19 vaccines. Grounded Theory approach guided the selection of informants, data collection, data analysis and report writing.
Results
Participants who delayed or refused COVID-19 vaccination exhibited a spectrum of vaccine-resistant and vaccine-hesitant attitudes which were weaved into the contexts of lacking decisional support from doctors, family and the government, and attitude roots of negative perception of ageing, fatalistic risk attitudes, low health literacy, present time perspective and negative values on western biomedicine, and reliance on the peripheral processing of vaccine-related information. While participants refused or delayed COVID-19 vaccination, they turned to alternative coping strategies to regain self-control in the pandemic.
Conclusions
Interventions to address vaccine hesitancy in older adults should focus on addressing attitude roots and strengthening the connectivity of older people with family, doctors, and government to engage older people in the vaccination decision making. Risk communication should shift to provide more personal relevant information in a caring style, meet older adults’ preference for peripheral information processing, and address their existing misperceptions about COVID-19 vaccines.
Deep Learning Identifies Intelligible Predictors of Poor Prognosis in Chronic Kidney Disease
Authors: Zhang Qingpeng , Liang Ping , Yang Jiannan , Wang Weilan , Yuan Guanjie , Han Min , Li Zhen 
Published: April 2022

Abstract
Purpose: To explore the performance and intelligibility of machine-learning and deep-learning models on end-stage renal disease (ESRD) prediction, based on readily-accessible clinical and laboratory features of patients suffering from chronic kidney disease (CKD). Materials and Methods: This single-center retrospective study included 2,382 patients diagnosed with CKD, of which 1,765 were included in the modelling analysis. Eight models (Logistic Regression (LR); Ridge Regression Classification (RRC); Least Absolute Shrinkage and Selection Operator (LASSO); Support Vector Machine (SVM) with a Gaussian kernel (SVM-RBF); and a linear kernel (SVM-Linear); Random Forest (RF); XGBoost; and Deep Neural Network (DNN)) were used to predict whether one person suffering from CKD would progress to ESRD within three years based on basic demographics, and clinical and comorbidity information. LASSO, RF, and XGBoost were introduced to screen the most significant markers to ESRD from the input features. For the DNN model, we introduced four advanced attribution methods (Integrated Gradients, DeepLIFT, GradientSHAP, and Feature Ablation) to enhance model intelligibility. Results: Age, follow-up duration, and 17 biochemical test outcomes (for instance, serum creatinine and hemoglobin) showed significant differences between patients in four CKD stages. The DNN model achieved an area under the receiver operating characteristic curve (AUC-ROC) of 0.8843, which was significantly higher than that of baseline models. Nonlinear machine learning models (SVM-RBF, RF, XGBoost, and DNN) generally outperformed linear ones (LR, RRC, LASSO, and SVM-Linear). The interpretation generated by DNN with attribution methods, RF, and XGBoost were consistent with clinical knowledge, whereas LASSO-based interpretation was inconsistent. Hematuria, proteinuria, potassium, urine albumin to creatinine ratio (ACR) were positively associated with the progression of CKD, while eGFR and urine creatinine were negatively associated with the progression of CKD. Hematuria is the most important independent risk predictor for the progression of diabetic nephropathy and urolithiasis. Conclusion: The adopted DNN with attribution algorithms extracted intelligible features of CKD progression. In addition, the DNN model identified a number of critical, but under-reported features, such as hematuria, that may be novel markers for the progression of CKD. This study provides physicians solid data-driven evidence in using machine learning and deep learning models for CKD clinical management and treatment.
Comparison of the Anti-Tumor Effect of Conditioned Media of Tonsil-Derived Mesenchymal Stem Cells on A549 and PANC-1 Cancer Cell Lines
Authors: Yüce Melek , Albayrak Esra , Gümüşkaptan Çağrı 
Published: April 2022

Abstract
Background Mesenchymal stem cells (MSCs) are the apple of the eye of cancer studies. It was indicated that the secreted factors, especially released by MSCs, have tumoral and anti-tumoral effects on tumor progression. MSCs obtained from different sources show different anti-tumoral effects. At the same time, MSCs originating from the same source also show different tumoral effects in different cancer cells. Here, we compared the anti-tumor effects of soluble factors secreted from MSCs derived from the palatine tonsil (T-MSC) as a new source of MSC on cancer cell lines A549 and PANC-1. Methods and Results Conditioned medium (CM) was obtained from T-MSCs that were successfully isolated from palatine tonsil tissue and characterized, and the cytotoxic effect of CM on the growth of A549 and PANC-1 at different concentrations was demonstrated by MTT analysis. In addition, the apoptotic effect of T-MSC-CM treatment was evaluated on cancer cells using Annexin-V/PI detection method by flow cytometry. Cell cycle arrest in cancer cells was analyzed by flow cytometry using propidium iodide (PI). As a result, we demonstrated that T-MSC-CM treatment significantly decreased the viability of A549 and PANC-1 cell lines in a dose dependent manner post 48 hours. Treatment of CM also differentially induced apoptosis on A549 and PANC-1 cells and caused G2/M arrest in cells. Conclusions In the light of these findings, our study is the first to show that T-MSC-CM has antitumor effect by stimulating apoptosis at different rates on A549 and PANC-1 cells, and has a cell-free cellular therapeutic potential.
Tectonic and velocity structure of the Gulf of Gökova, southwest Anatolia, determined by double-difference seismic tomography: the 20 July 2017 Mw6.5 Bodrum Earthquake
Authors: Özdemirli-Esat Tuğba , Doğanay-Özkan Melike , Esat Korhan 
Published: April 2022

Abstract
The 20 July 2017 Mw6.5 Bodrum main shock that occurred in the Gulf of Gökova is one of the largest earthquakes in the north-south extensional Aegean region. In this study, we generated the first three-dimensional local earthquake tomography for the Gökova region using the double-difference tomography algorithm to provide a contribution to discussions on the source fault of the main shock. The 2500 earthquake records of the magnitude ≥ 2.3 between 01 January 2017 and 31 December 2017 were chosen from the 18 strong-motion accelerometer and 12 weak-motion seismometer stations. The P- and S-wave velocity models and Vp/Vs ratio model of the study area were created, and 1442 earthquakes were relocated. With the help of seismic tomographic imaging, we concluded that the main shock occurred on the south-dipping Gökova Fault Zone, not on the so-called north-dipping fault plane suggested in some studies. We also determined that some earthquakes that occurred in 2017 were associated with a north-dipping normal fault observed up to 10 km depth immediately north of the Gökova Fault Zone. Additionally, the Datça-Kale Main Breakaway Fault, which separates the sedimentary cover and the basement rocks, was also determined in the tomographic profiles. The kinematic analysis of moment tensor solutions of the earthquakes with magnitudes greater than 4, twenty-one of which were generated in this study, is consistent with the north-south extensional tectonic regime in the Aegean region.
Impact of ICU bed availability on ovarian cancer surgical hospitalization rates during the first wave of the COVID-19 pandemic: an ecological study.
Authors: Moterani Vinicius Cesar , Junior Nino Jose Wilson Moterani , Pimentel Franklin Fernandes , Reis Francisco Jose Candido dos 
Published: April 2022

Abstract
BackgroundThe COVID-19 pandemic, which began in 2020, disrupted healthcare services. Reports of changes in surgical activity coincide with the outbreak period. We aimed to identify if any changes could be determined in hospitalization rates of ovarian cancer patients from 2016 to 2020, comparing pre-pandemic and pandemic levels. ResultsHospitalization rates in the state fell, coinciding with the pandemic. Surgical hospitalization rate ratios were inversely correlated with COVID-specific ICU bed occupation rates during the third trimester of 2020, with a Pearson Correlation coefficient of -0.50 (95% CI: -0.78 to -0.05, p = 0.03). Observed ovarian cancer hospitaliation rates were 85% compared to expected values if pre-pandemic trends were mantained.ConclusionA 15% reduction on ovarian cancer hospitalization rates was identified in this scenario. ICU availability was inversely correlated with ovarian cancer surgical activity. These results demonstrate the impact of the COVID-19 pandemic on the treatment of conditions that compete for the same healthcare resources.
Method for constructing human iPS cell line RZPBi001 from Peripheral Blood Cells
Authors: Chen Xiaoqing , Luan yuling , Ying Rong , Yuan Weiming , Tian Pengfei , Hua Dongming , Feng Yu 
Published: April 2022

Abstract
Patient-derived hiPSCs could generate indefinitely illness-related cells and organoids, allowing research scientist to reproduce some pathologic change of human sickness models in petri dishes. Whereas, dermal fibroblasts require some traumatic injury to obtain and have the risky of accumulating variations due to unveil to external stimulus such as UV light. Meanwhile, peripheral blood is regarded to be a more excellent source of somatopiasm because it is less damage to collect and its mutation load is significantly lower than that of skin tissue.In this paper, we describe the successful construction of non-integration hiPSCs form human peripheral blood cells by Sendai virus. A healthy adult female of Han nationality from China contributed her peripheral blood mononuclear cells (PBMCs). Non-integrating Sendai virus recombinant PBMCs with human OSKM (Oct4, Sox2, Kl4 and c-Myc) transcription factors.The pluripotency of transgenic-free iPSCs was confirmed by immunofluorescence staining of pluripotency markers and the capacity of iPSCs to initially differentiate into 3 germ layers in vitro. Moreover, iPSC lines demonstrated awfully normal karyotypes. In the research of illness progression and pathogenesis (eg, DMD, SMA, ALS), iPSC lines can be used as controls.
Two-dimensional multiferroic material of metallic p-doped SnSe
Authors: Du Ruofan , Wang Yuzhu , Cheng Mo , Wang Peng , Li Hui , Feng Wang , Song Luying , Shi Jianping , He Jun 
Published: April 2022

Abstract
Two-dimensional (2D) multiferroic materials have garnered broad interests attributed to their fascinating magnetoelectric properties and energy-efficient multifunctional device applications. Multiferroic heterostructures have recently been realized, nevertheless, the direct coupling between ferroelectric and ferromagnetic order in a single material still remains a daunting challenge, especially for 2D materials. Herein, we develope a simple physical vapor deposition approach to synthesize 2D p-doped SnSe. The local phase segregation of SnSe2 microdomains and accompanying interfacial charge transfer result in the emergence of degenerate semiconductor and metallic feature in SnSe. Intriguingly, the room-temperature ferrimagnetism has been demonstrated first in 2D p-doped SnSe with the Curie temperature approaching to ~337 K. Meanwhile, the robust in-plane ferroelectricity is maintained even under the depolarizing field introduced by SnSe2. The coexistence of ferrimagnetism and ferroelectricity in 2D metallic p-doped SnSe verifies its multiferroic feature. This work presents a significant advance for exploring the magnetoelectric coupling in 2D limit and constructing high-performance logic devices to extend Moore's law.
Exploring a better option for the treatment of autoimmune pulmonary alveolar proteinosis: whether GM-CSF is combined with WLL: a meta-analysis.
Authors: Liu Xiaohong , Cui Jian , Liu Shixu , Xiong Mengran , Zhang Jinzhi , Liu Yanyi , Xia Xiao , Li Guangxi 
Published: April 2022

Abstract
Background
Understanding the efficacy and safety of treatment methods related to Autoimmune Pulmonary alveolar proteinosis (aPAP) will help provide information for selecting clinical trials. Our objective was to assess the efficacy and safety of granulocyte macrophage colony stimulating factor (GM-CSF) combined with Whole lung lavage (WLL) in aPAP using a systematic review and meta-analysis of the literature.
Methods
PubMed/ MEDLINE, EmBase, Web of Science and Cochrane Collaboration databases were systematically searched. The search included literature published or presented from inception to February 2022.
Results
The main outcomes were response rate, relapse rate, alveolar-arterial oxygen gradient (P(A-a)O2), alveolar oxygen partial pressure (PaO2), diffusing capacity of the lung for carbon monoxide (DLCO), forced vital capacity (FVC) and 6 min’ walk distance (6MWD). Of 1347 studies yielded by the search, 18 were retained, totalling 477 patients (48 treated with the combination therapy vs 429 receiving GM-CSF alone). Compared with GM-CSF alone, the clinical treatment of GM-CSF combined with WLL was more effective, including significantly higher response rate (98.6% vs. 73.9%, P = 0.018), lower relapse rate (3% vs. 29%, P < 0.001), more improvements in P(A-a)O2 (41.93 mmHg vs. 7.44 mmHg, P < 0.001), PaO2 (26.6 mmHg vs. 10.29 mmHg, P < 0.001), DLCO (10.88% vs. 7.94%, P = 0.223), FVC (10.4% vs. 2.62%, P < 0.001) and 6MWD (100.6 m vs. 52.25 m, P = 0.077).
Conclusions
This meta-analysis found superior efficacy of GM-CSF combined with WLL than GM-CSF alone and highlighted the benefit of the combination of GM-CSF and WLL therapy of PAP. Specially, GM-CSF combined with WLL therapy showed a higher response rate, more improvements on gas exchange and pulmonary function, suggesting combination therapy could have more benefits on aPAP patients. These data provided convincing evidence that GM-CSF combined with WLL could be a promising treatment option for aPAP.
Trial registration:
A protocol of this meta-analysis has been registered on PROSPERO (registration number: CRD42022306244); registered on 22 February 2022.