bluuebunny/crossref_metadata_2025_split huggingface dataset:
CIRCADIAN RHYTHMS AND PERSONALIZED STRATEGIES FOR ANTI-AGING THERAPIES
Authors: Kalmukova Olesia , Kyryk Vitalii , Dzerzhynsky Mykola 
Published: July 2022

Background. Nowadays personalized medicine is actively developing and consists of individual approaches during patients' treatment, diagnoses and prognoses. Since the first use of DNA sequence analysis in 2009, many other directions and methods for precision medicine have been proposed, including metabolome, transcriptome, proteome, microbiome analysis etc., which reflect internal factors of organisms. Moreover, to take into account environmental influence on organisms including day/night activity, feeding and physical training regime, it was proposed to apply the descriptions of circadian system rhythmicity of each patient. Also, with organism aging, the sensitivity to external factors is raised that emphasizes the importance of the chronobiological approach in anti-aging concept. In this review we discussed available ways of the application of circadian system parameters to analyze human metabolic state.
Methods. Search strategy: PubMed, Scopus, DOAJ (Directory of Open Access Journals) and Google Scholar were used to search for original research and articles review; no abstracts from meeting reports have been cited. ClinicalTrials.gov was used to search for clinical studies. Search terms included “chronotherapy”, “circadian system”, and “chronobiology”.
Results. According to personalized medicine, the analysis of circadian system in the case of each patient is necessary as circadian rhythmicity varies in every person. Taking into account the peculiarities of patient’s circadian system it will be easy to choose the best time for drug administration resulting in high efficacy and low side effects. The analysis of circadian system can be performed on molecular, physiological and systemic (general, metabolic and inflammation markers) levels. There was shown the increase in the number of clinical trials which are based on the use of chronobiological approach during the treatment of different pathologies that increase with aging: depression, insomnia, metabolic and cardiovascular disease, cancer. More than 1,000 clinical trials involving circadian interventions and chronobiology have been registered worldwide.
Conclusion. Chronobiological approach can be used as an additional measure to anti-aging therapy to diagnose metabolic state, to choose more effective treatment time as well as in preventive healthcare in terms of personalized medicine.
REGENERATIVE EFFECTS OF MOUSE ADIPOSE-DERIVED MULTIPOTENT STROMAL CELLS IN A MICROMASS GRAFT FOR THE TREATMENT OF BONE INJURY MODEL
Authors: Kyryk Vitalii , Kuchuk Olga , Klymenko Pavlo 
Published: July 2022

Background. Adipose-derived stem cells (ADSCs) are a promising source for the regeneration of bone tissue injuries. At the same time, three-dimensional cultures provide spatial organization of stem cells for optimal intercellular signaling, contact interaction and increase the efficiency of directed osteogenic differentiation prior to further transplantation.
The aim of the study was to establish the regenerative potential of mouse adipose-derived stem cells in micromass grafts differentiated into the osteogenic direction to restore the bone injury in mice.
Methods. Three-dimensional micromass cultures of murine ADSCs with further differentiation into osteogenic direction were obtained. The migration potential of cells from micromass in vitro and the effectiveness of differentiation by staining for alkaline phosphatase were evaluated. Mice with the model of femoral bone injury were transplanted with ADSCs micromass grafts and 21 days later the lesion site was examined by histological and morphometric methods.
Results. The protocols for the cultivation and directed osteogenic differentiation of ADSCs in the three-dimensional micromass culture have been developed. Alkaline phosphatase production was demonstrated in cells that migrated from micromass, confirming the effectiveness of differentiation. In macroscopic examination 21 days after graft transplantation, the defect sites in femur were filled with dense tissue, while in control bones without the use of transplants, the size of the defect by 80 ± 6 % corresponded to the initial diameter and depth of injury. Histological examination of femoral bone lesions in the area of transplantation of micromass grafts revealed the formation of granulation tissue followed by the replacement of defects with newly formed bone tissue with thickening of periosteum and compact bone substance, similar to callus in fracture regeneration. In animals that underwent transplantation of micromass without prior osteogenic differentiation, the diameter of the zone of active regeneration of the diaphysis at the site of injury was 1.3 ± 0.2 mm while in the group with transplantation of directed differentiated graft it was significantly lower (0.37 ± 0.12 mm, p ≤ 0.05).
Conclusions. Three-dimensional grafts of adipose-derived multipotent mesenchymal stromal cells cultured in micromass are able to improve bone tissue regeneration in a model of bone injury in mice. In this case, the grafts differentiated into osteogenic direction, provide better morphological indicators of bone recovery, compared with the micromass without prior differentiation.
Quality in higher education: an evaluation in a graduation course in administration at a large university in the municipality of São Paulo
Authors: Silva Alexandre Mendes da , Gouvêa Maria Aparecida , Semensato Bárbara Ilze , Pereira Fernando Antonio de Melo 
Published: July 2022

Objective: to assess the quality perceived by students in the on-site undergraduate course in administration at a private university located in the city of São Paulo, using a new scale developed from the skills expected in the training of future administrators.
Design/Methodology/Approach: a 46-item questionnaire was developed and tested, with multivariate statistical techniques (exploratory factor analysis and Cronbach's alpha). In total, 629 students participated in the study.

Results: the study identified four blocks of competences: managerial skills, problem solving, proactivity, communication, and creativity and techniques. In the global assessment of the quality of educational services, the institution achieved 76.19%, which indicates that the Administration course offered by the institution can be considered of good quality. The work delivers an instrument and an evaluation methodology that can be used to improve administration courses in the country.

Originality/value: the study is relevant when assessing the quality of higher education from a new scale based on the skills required to exercise the profession of an administrator defined by Resolution No. 4 of July 13th, 2005.
Satisfação no trabalho e retenção de profissionais em organizações de TI públicas e privadas
Authors: Steil Andrea Valéria , Bello Janine da Silva Alves , Cuffa Denise de , Freitas Aline Ferreira de 
Published: July 2022

Objetivo: Examinar a relação da satisfação global no trabalho, assim como das dimensões da satisfação (satisfação com a natureza do trabalho, satisfação com os colegas, satisfação com a chefia, satisfação com o salário, satisfação com as promoções), com a retenção de profissionais de TI em organizações de TI públicas e privadas.

Design/metodologia/abordagem: Participaram da pesquisa 252 profissionais (139 de organizações públicas e 113 de organizações privadas) de organizações localizadas no estado de Santa Catarina, Brasil. Os dados foram coletados por meio de survey e analisados por meio do teste estatístico de Spearman.

Resultados: Não houve relação positiva entre a medida global de satisfação dos profissionais de TI e das dimensões satisfação com colegas, satisfação com a chefia, satisfação com a natureza do trabalho e satisfação com as promoções e a sua retenção. Apenas a satisfação com o salário apresentou relação positiva com a retenção dos profissionais nas organizações de TI públicas e privadas.

Implicações práticas: Os resultados encontrados geram insumos para o estabelecimento de políticas de retenção dos profissionais de TI, especialmente relacionadas ao salário.

Originalidade/valor: Este estudo investigou não apenas a medida global de satisfação com o trabalho, mas também as suas dimensões. O estudo demonstra, de forma inédita, que a medida global de satisfação com o trabalho não retém profissionais de TI em organizações de TI públicas e privadas e que estratégias de retenção precisam focar especialmente na satisfação desses profissionais com o seu salário.
Compensation for radiotherapy treatment interruptions due to a cyberattack: An isoeffective DVH‐based dose compensation decision tool
Authors: O'Shea Katie , Coleman Linda , Fahy Louise , Kleefeld Christoph , Foley Mark J. , Moore Margaret 
Published: July 2022

AbstractUnscheduled interruptions to radiotherapy treatments lead to decreased tumor control probability (TCP). Rapid cell repopulation in the tumor increases due to the absence of radiation dose, resulting in the loss of TCP. Compensation for this loss is required to prevent or reduce an extension of the patient's overall treatment time and regain the original TCP. The cyberattack on the Irish public health service in May 2021 prevented radiotherapy treatment delivery resulting in treatment interruptions of up to 12 days. Current standards for treatment gap calculations are performed using the Royal College of Radiologists (RCR) methodology, using a point‐dose for planning target volume (PTV) and the organs at risk (OAR).An in‐house tool, named EQD2VH, was created in Python to perform treatment gap calculations using the dose–volume histogram (DVH) information in DICOM data extracted from commercial treatment planning system plans. The physical dose in each dose bin was converted into equivalent dose in 2‐Gy fractions (EQD2), accounting for tumor cell repopulation. This EQD2‐based DVH provides a 2D representation of the impact of treatment gap compensation strategies on both PTV and OAR dose distributions compared to the intended prescribed treatment plan. This additional information can aid clinicians’ choice of compensation options. EQD2VH was evaluated using five high‐priority patients experiencing a treatment interruption when the cyberattack occurred. Compensation plans were created using the RCR methodology to evaluate EQD2VH as a decision‐making tool.The EQD2VH method demonstrated that the comparison of compensated treatment plans alongside the original intended treatment plans using isoeffective DVH analysis can be achieved. It enabled a visual and quantitative comparison between treatment plan options and provided an individual analysis of each structure in a patient's plan. It demonstrated potential to be a useful decision‐making tool for finding a balance between optimizing dose to PTV while protecting OARs.
Chondro‐Inductive b‐TPUe‐Based Functionalized Scaffolds for Application in Cartilage Tissue Engineering
Authors: Martínez‐Moreno Daniel , Venegas‐Bustos Desiré , Rus Guillermo , Gálvez‐Martín Patricia , Jiménez Gema , Marchal Juan Antonio 
Published: July 2022

AbstractOsteoarthritis is a disease with a great socioeconomic impact and mainly affects articular cartilage, a tissue with reduced self‐healing capacity. In this work, 3D printed 1,4 butanediol thermoplastic polyurethane (b‐TPUe) scaffolds are functionalized and infrapatellar mesenchymal stem cells are used as the cellular source. Since b‐TPUe is a biomaterial with mechanical properties similar to cartilage, but it does not provide the desired environment for cell adhesion, scaffolds are functionalized with two methods, one based on collagen type I and the other in 1‐pyrenebutiric acid (PBA) as principal components. Alamar Blue and confocal assays display that PBA functionalized scaffolds support higher cell adhesion and proliferation for the first 21 days. However, collagen type I functionalization induces higher proliferation rates and similar cell viability than the PBA method. Further, both functionalization methods induce extracellular matrix synthesis, and the presence of chondrogenic markers (Sox9, Col2a, and Acan). Finally, SEM images probe that functionalized 3D printed scaffolds present much better cell/biomaterial interactions than controls and confirm early chondrogenesis. These results indicate that the two methods of functionalization in the highly hydrophobic b‐TPUe enhance the cell‐biomaterial interactions and the improvement in the chondro‐inductive properties, which have great potential for application in cartilage tissue engineering.
Fabrication and Electrochemical Performance of TiO2–TiN/Sn–SnO2 Composite Films on Ti for LIB Anodes with High Capacity and Excellent Conductivity
Authors: Chen Xuewen , Kure‐Chu Song‐Zhu , Matsubara Takashi , Hihara Takehiko , Yashiro Hitoshi , Okido Masazumi 
Published: July 2022

AbstractAccording to the requirements for anode materials of safe and high‐capacity lithium‐ion batteries (LIBs), various Sn/TiO2 and SnO2/TiO2 composite materials have been developed, but no reports have yet combined them by a simple fabrication method. Herein, a novel and simple approach is reported for the fabrication of nanoporous TiO2–TiN/Sn–SnO2 composite films by successive Ti anodization and cathodic hybrid electrodeposition of Sn in a nitric acid‐based aqueous solution containing Sn2+ and/or Sn4+ ions. The conductivity of the anodic TiO2–TiN composite films facilitates the simultaneous electrodeposition of metallic Sn and electrophoretic deposition of SnO2 colloids, both on the surface and inside the nanopores of the film. Notably, the nanoporous structure is preserved, which provides a large surface area. The electrodeposition of Sn–SnO2 nanoparticles on the nanoporous TiO2–TiN film significantly improves the charge transfer and conductivity of the composite film and enhances the discharge capacity to up to 2386.1 µAh cm−2 (approximately sixfold higher than that of the bare matrix film).
Archaeometallurgical characterisation of ancient copper slags from pre‐Harappan site, Kunal, India
Authors: Kanth Aditya Prakash , Singh Manager Rajdeo , Mani Buddha Rashmi 
Published: July 2022

AbstractThe copper slags collected during the excavation of the Early Harappan period site at Kunal in northern India were studied to understand the advancement of smelting technology and the achieved smelting temperature in the furnace by undertaking archaeometallurgical characterisation of the slags. In this research, two types of slags such as slag with glassy appearance and granulated slag were selected for the study. The microscopic structure and distribution of slag components were investigated using optical microscopy and phase determination was done by X‐ray diffraction (XRD). Chemical characterization of the slags was conducted using scanning electron microscopy–energy‐dispersive X‐ray spectroscopy, X‐ray fluorescence analysis (XRF) and inductively coupled plasma–mass spectrometry to build a complete chemical profile of the slags. Fayalite and magnetite were the dominant phases in the glassy slag; however, the granulated slag showed the dominance of calcite as secondary phase which reflected the dolomitic stoichiometry of the slag. The presence of dominant fayalite mineral phase as detected by XRD and higher concentration of iron as detected by XRF indicated the reducing environment during the smelting process. In this study, the absence of sulphur is reported which is unique to the ancient copper slag.
Are there long‐living Hilda‐like asteroids between Jupiter and Saturn?
Authors: Dvorak Rudolf , Kubala Michael 
Published: July 2022

AbstractTo clarify the question of asteroids between Jupiter and Saturn a numerical study of the stability of orbits of fictitious bodies in this region has been undertaken. There are almost no minor planets moving there with the exception of 61 faint asteroids. After determining the mean motion resonances and the three‐body resonances, we have studied orbits for inclinations from 1 to 91 degrees to find out stable regions from 6.8 to 8.0 au. This was done up to 100 Myrs in the dynamical model Sun–Jupiter–Saturn and massless fictitious asteroids. In a study with a finer grid in inclination from 1 to 31 degrees and semi‐major axes from 6.88 to 7.30 au, we integrated ten thousand orbits up to the moment of their escape. These results showed a region in semi‐major axes with stable orbits in the interval from 7.12 to 7.23 au for moderate inclinations. Nevertheless, being a temporary capture there could be these objects much larger than the tiny ones discovered up to now in the region between Jupiter and Saturn.
The role of USP51 in attenuating chemosensitivity of lung cancer cells to cisplatin by regulating DNA damage response
Authors: Zhou Feng , Lu Jinchang , Jin Wei , Li Zhanbo , Xu Donghui , Gu Liang 
Published: July 2022

AbstractLung cancer is the most frequent type of cancer affecting both men and women globally, and it is associated with a high mortality rate. It is clinically treated with cisplatin, a platinum‐based drug that works by generating DNA lesions, which activates DNA damage response and induces cell death. However, chemoresistance by cancer cells limits the clinical usefulness of cisplatin as an anticancer drug. Here, we uncovered a role of ubiquitin‐specific protease 51 (USP51) in the chemosensitivity of lung cancer cells to cisplatin by regulating DNA damage response. USP51 was more upregulated in lung cancer tissues of chemotherapy‐resistant patients than those of chemotherapy‐sensitive patients with adjacent, nontumor tissues. USP51 overexpression in lung cancer cells in vitro reduced γ‐H2AX formation and promoted checkpoint kinase 1 (CHK1) phosphorylation, whereas USP51 knockdown showed opposite effects, indicating that USP51 played an important role in promoting DNA damage repair. Finally, USP51 knockdown weakened cisplatin resistance in A549/DDP cells and significantly suppressed tumor growth in vivo, suggesting that a USP51 inhibitor combined with cisplatin may be considered as an effective treatment strategy to eliminate drug‐resistant lung cancer cells.
Exploration of critical thinking and self‐regulated learning in online learning during the COVID‐19 pandemic
Authors: Anwar Yunita Arian Sani , Muti'ah Muti'ah 
Published: July 2022

AbstractAn in‐depth analysis of today's online biochemistry learning is required to ensure better learning in the future. As a skill that students need to enter the world of work, critical thinking remains a goal in higher education. To develop critical thinking, students need to self‐regulate by developing their self‐regulated learning (SRL). This study aims to analyze students' critical thinking and SRL during online biochemistry learning. The research sample comprises 54 University of Mataram students. The data on critical thinking and SRL were gathered using tests and questionnaires. Supporting data were collected from observations on the Moodle platform, which was used as a learning tool during the learning process, and free‐response data. The results revealed low average scores for three components of the students' critical thinking in online biochemistry learning, namely hypothesis testing, developing conclusions and argument analysis. Among the indicators, metacognitive skill had the highest average score and help seeking had the lowest average score in the SRL measurement. Students' low critical thinking in online biochemistry learning may be caused by a lack of student–student interaction.
Green supply chain management in manufacturing firms: A resource‐based viewpoint
Authors: Khan Mehmood , Ajmal Mian M. , Jabeen Fauzia , Talwar Shalini , Dhir Amandeep 
Published: July 2022

AbstractAs sustainability draws increasing attention due to the COVID‐19 pandemic, interest in the green supply chain has likewise substantially increased. The present study contributes deeper insights into the logical integration of the resource‐based characteristics that can facilitate the effective transition from traditional to green supply chains while also increasing the effectiveness of existing green supply chains. The proposed model, rooted in the resource‐based view (RBV) theory, examines the associations of supply chain connectivity (SCC), supply chain information sharing (SCIS), top management commitment (TMC) and green procurement and logistics acceptance (GPLA) with green supply chain management (GSCM). Analysis of data from employees at 381 manufacturing firms in the United Kingdom confirmed the positive associations of SCC and SCIS with TMC and GPLA, of TMC with GPLA and GSCM and of GPLA with GSCM. The results also confirmed the serial mediation effect of TMC and GPLA on the associations of both SCC and SCIS with GSCM. The study offers several practical insights and theoretical contributions, including a novel GSCM scale.
Low ferroptosis score predicts chemotherapy responsiveness and immune‐activation in colorectal cancer
Authors: Lv Yang , Feng Qing‐Yang , Zhang Zhi‐Yuan , Zheng Peng , Zhu De‐xiang , Lin Qi , Chen Si‐min , Mao Yi‐Hao , Xu Yu‐Qiu , Ji Mei‐ling , Xu Jian‐Min , He Guo‐dong 
Published: July 2022

AbstractBackgroundExisting studies for ferroptosis and prognosis in colorectal cancer (CRC) were limited. In this study, we aim to investigate the prognostic role of ferroptosis markers in patients with CRC and exploration of its micro‐environmental distributions.MethodsImmunohistochemical staining was performed for CRC patients’ tissue microarray. Selection and prognostic validation of markers were based on mRNA data from the cancer genome atlas (TCGA) database. Gene Set Enrichment Analysis (GSEA) was performed to indicate relative immune landmarks and hallmarks. Ferroptosis and immune contexture were examined by CIBERSORT. Survival outcomes were analyzed by Kaplan‐Meier analysis and cox analysis.ResultsA panel of 42 genes was selected. Through mRNA expression difference and prognosis analysis, GPX4, NOX1 and ACSL4 were selected as candidate markers. By IHC, increased GPX4, decreased NOX1 and decreased FACL4 indicate poor prognosis and worse clinical characteristics. Ferroptosis score based on GPX4, NOX1 and ACSL4 was constructed and validated with high C‐index. Low ferroptosis score can also demonstrate the better progression free survival and better adjuvant chemotherapy (ACT) responsiveness. Moreover, tumor with low ferroptosis score tend to be infiltrated with more CD4+ T cells, CD8+ T cells and less M1 macrophage. Finally, we found that IFN‐γ was potentially the central molecule at the crossroad between ferroptosis and onco‐immune response.ConclusionFerroptosis plays important role on CRC tumor progression, ACT response and prognosis. Ferroptosis contributes to immune‐supportive responses and IFN‐γ was the central molecule for this process.
Catalytic Hydrogenolysis of the Pt−OPh Bond of a Molecular Pt(II) Complex using Silica Supported Pd, Rh and Pt Nanoparticles
Authors: Luo Zhongwen , Xie Jiahan , Kaylor Nicholas , Dickie Diane A. , Ketcham Hannah E. , Davis Robert J. , Gunnoe T. Brent 
Published: July 2022

AbstractSilica‐supported Pd, Rh and Pt metal nanoparticles catalyze the hydrogenolysis of the Pt−OPh bond of (tbpy)Pt(OPh)Cl to release PhOH. Based on kinetic studies monitored by 1H NMR spectroscopy, the reactivity trend is Pd>Rh>Pt. Kinetic studies with Pd/SiO2 are consistent with a first‐order dependence on the catalyst and the molecular Pt(II) complex (tbpy)Pt(OPh)Cl. Using TEM‐EDS mapping and ICP‐OES measurements of a recovered Pd catalyst, after 1 hour of hydrogenolysis of (tbpy)Pt(OPh)Cl, approximately 10–16 % Pt deposition (relative to Pd mol %) on the Pd/SiO2 surface was quantified.