bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pure Metal Clusters with Atomic Precision for Nanomanufacturing
Authors: Wu Haiming , Zhang Hanyu , Geng Lijun , Jia Yuhan , Huang Benben , Yang Mengzhou , Yin Baoqi , Lei Xin , Luo Zhixun 
Published: May 2022

AbstractAdvances in cluster science have enabled the preparation of atomically precise metal clusters with one to a hundred atoms under controllable expansion conditions. After introducing typical gas-phase cluster preparation and reaction apparatuses, this work summarized recent progress in preparing pure metal clusters of single-atom resolution, including neutral and ionic ones, with typical examples of Al, V, Nb, Fe, Co, Ni, Rh, Pt, Ag, Cu, and Pb. With the development of soft-landing deposition technology, the size-selective pure metal clusters with strict atomic precision and predictive property will benefit nanomanufacturing down to atomic and near-atomic scales. This work serves as a modest motivation to stimulate the interest of scientists focusing on interdisciplinary subjects.
Trajectory of patients consulting the emergency department for high blood pressure values
Authors: Vadeboncoeur Alain , Marcil Marie-Joelle , Cyr Samuel , Gupta Mona , Cournoyer Alexis , Minichiello Anthony , Larose Dominic , Sirois-Leclerc Julie , Tardif Jean-Claude , Morin Josée , Masson Violaine , Cossette Mariève , Brouillette Judith 
Published: May 2022

Abstract
Objectives
Emergency department (ED) visits for high blood pressure are increasing in frequency. We aimed to map those patients’ trajectory, from referral sources to the type of care received at the ED to anticipated actions for future high blood pressure concerns, and to better understand their reasons for consulting the ED for high blood pressure values.

Methods
Between 2018 and 2020, patients who presented to the Montreal Heart Institute’s ED for elevated blood pressure were recruited in a prospective observational study including a post hoc structured telephone interview and medical chart review. Five possible referral sources were predetermined. We provided proportions and 95% confidence intervals.

Results
A total of 100 patients were recruited (female: 59%, mean age: 69 ± 12). A majority (93%, 95% CI 88–98%) possessed a home blood pressure device, among which 46% (95% CI 36–56%) remembered receiving advice for its use. The main referral sources for high blood pressure to the ED were self-reference (53%, 95% CI 43–63%), advice of a lay person (19%, 95% CI 11–27%) or a nurse (13%, 95% CI 6–20%). Mainly, patients reported being concerned by concomitant symptoms or experiencing acute medical consequences (44%, 95% CI 34–54%), having followed the recommendation of a third party (33%, 95% CI 24–42%), or having concerns about their medication (6%, 95% CI 1–11%). Two weeks following their ED visits, consulting ED remained the main choice for future concerns about high blood pressure for 27% of participants. When specifically asked if they would return to the ED for elevated blood pressure, 73% (95% CI 64–83%) said yes.

Conclusions
Most patients who consulted the ED for elevated blood pressure values were self-referred. More can be done to promote blood pressure education, effective use of personal blood pressure devices, and recommendations for patients and health professionals when confronted with high blood pressure results.
Electrical Conductivity of Clayey Rocks and Soils: A Non‐Linear Model
Authors: Qi Youzheng , Wu Yuxin 
Published: May 2022

AbstractIt is well‐accepted that Archie's law is only applicable to “clean” rocks and soils but fails in “dirty” ones where clay minerals possess an additional component of surface conductivity. Although several models, for example, Waxman‐Smits model, were presented to account for this phenomenon, surface conductivity is always inappropriately treated as constant, which actually only holds at high salinities. The essential non‐linear characteristic differing over fluid salinities has not been physically or mathematically explained well in those models. We scrutinize the conduction mechanism of clayey rocks and soils and ascribe this non‐linear feature to (a) variation of the electrical double layer and (b) the intrinsic clay‐and‐water conduction pattern. With effective medium theory, we develop an easy‐to‐use non‐linear model that both reflects electrochemical theories and explains the measurement data well. Our model can be used to produce more accurate results for laboratory‐ and field‐scale petrophysical parameter evaluations than the previous models.
Strong Long‐Lived Anticyclonic Mesoscale Eddies in the Balearic Sea: Formation, Intensification, and Thermal Impact
Authors: Aguiar E. , Mourre B. , Alvera‐Azcárate A. , Pascual A. , Mason E. , Tintoré J. 
Published: May 2022

AbstractAnticyclonic mesoscale eddies are often observed in the Balearic Sea (BS) toward the end of summer and autumn. In some years, these eddies become strong and persistent, modifying the local water mass properties. In this study, we analyze two of the most significant recent long‐lived anticyclonic eddies, occurring in 2010 and 2017, using data from a high‐resolution circulation model, altimetry and satellite‐borne sea surface temperature observations. These eddies lasted around 2 and 4 months, respectively, with a radius varying between 40 and 75 km. The generation and intensification mechanisms of these long‐lived anticyclonic eddies are studied by means of (a) energy conversion terms associated with eddy‐mean flow interaction and (b) model sensitivity tests. Results show that these eddies were formed and intensified through mixed barotropic and baroclinic instabilities. The former are produced under the action of intense northwesterly (NW) winds. The latter are related to the existence of an intense summer thermal front between the BS and the Gulf of Lion, and to northward inflows of relatively lower salinity waters. Both the wind events and the presence of the thermal front are necessary for the formation of the eddies. The intensification process varied between both events. While in 2010 it was driven by significant salinity gradients produced by northwards inflows, in 2017 it was produced by additional intense NW winds. Both long‐lived anticyclonic eddies created long‐lasting surface temperature anomalies up to 2.5°C, which have characteristics of local marine heatwaves.
Northern Mid‐Latitude Mesospheric Cloud Frequencies Observed by AIM/CIPS: Interannual Variability Driven by Space Traffic
Authors: Stevens Michael H. , Randall Cora E. , Carstens Justin N. , Siskind David E. , McCormack John P. , Kuhl David D. , Dhadly Manbharat S. 
Published: May 2022

AbstractRecent advances in data processing from the Cloud Imaging and Particle Size (CIPS) instrument on the NASA Aeronomy of Ice in the Mesosphere satellite allow observation of bright mesospheric clouds at mid‐latitudes (<60°). When adjusted for the evolving local time (LT) of the CIPS observations during its mission we find that the frequencies of these bright clouds in the northern hemisphere show no trend from 2007 to 2021 and no dependence on the solar cycle, although the interannual variability is extreme. Rather we investigate the possible link with propellant exhaust from orbital vehicles, typically launched at lower latitudes. By filtering the launch record equatorward of 60°N using only those launches between 23 and 10 LT, we find a strong correlation with the observed mid‐latitude mesospheric cloud frequency variability between 56° and 60°N. Meridional winds at 92 km from a meteorological analysis system reveal that these morning launches occurred at the time of maximum northward transport. Based upon this combination of high correlation between the cloud frequency and the launch record plus favorable transport conditions, it is likely that space traffic has a strong influence on the interannual variability of these bright mesospheric clouds.
RUNX1 mutations contribute to the progression of MDS due to disruption of antitumor cellular defense: a study on patients with lower-risk MDS
Authors: Kaisrlikova Monika , Vesela Jitka , Kundrat David , Votavova Hana , Dostalova Merkerova Michaela , Krejcik Zdenek , Divoky Vladimir , Jedlicka Marek , Fric Jan , Klema Jiri , Mikulenkova Dana , Stastna Markova Marketa , Lauermannova Marie , Mertova Jolana , Soukupova Maaloufova Jacqueline , Jonasova Anna , Cermak Jaroslav , Belickova Monika 
Published: May 2022

AbstractPatients with lower-risk myelodysplastic syndromes (LR-MDS) have a generally favorable prognosis; however, a small proportion of cases progress rapidly. This study aimed to define molecular biomarkers predictive of LR-MDS progression and to uncover cellular pathways contributing to malignant transformation. The mutational landscape was analyzed in 214 LR-MDS patients, and at least one mutation was detected in 137 patients (64%). Mutated RUNX1 was identified as the main molecular predictor of rapid progression by statistics and machine learning. To study the effect of mutated RUNX1 on pathway regulation, the expression profiles of CD34 + cells from LR-MDS patients with RUNX1 mutations were compared to those from patients without RUNX1 mutations. The data suggest that RUNX1-unmutated LR-MDS cells are protected by DNA damage response (DDR) mechanisms and cellular senescence as an antitumor cellular barrier, while RUNX1 mutations may be one of the triggers of malignant transformation. Dysregulated DDR and cellular senescence were also observed at the functional level by detecting γH2AX expression and β-galactosidase activity. Notably, the expression profiles of RUNX1-mutated LR-MDS resembled those of higher-risk MDS at diagnosis. This study demonstrates that incorporating molecular data improves LR-MDS risk stratification and that mutated RUNX1 is associated with a suppressed defense against LR-MDS progression.
Cytoglobin attenuates pancreatic cancer growth via scavenging reactive oxygen species
Authors: Hoang Dinh Viet , Thuy Le Thi Thanh , Hai Hoang , Hieu Vu Ngoc , Kimura Kenjiro , Oikawa Daisuke , Ikura Yoshihiro , Dat Ninh Quoc , Hoang Truong Huu , Sato-Matsubara Misako , Dong Minh Phuong , Hanh Ngo Vinh , Uchida-Kobayashi Sawako , Tokunaga Fuminori , Kubo Shoji , Ohtani Naoko , Yoshizato Katsutoshi , Kawada Norifumi 
Published: May 2022

AbstractPancreatic cancer is a highly challenging malignancy with extremely poor prognosis. Cytoglobin (CYGB), a hemeprotein involved in liver fibrosis and cancer development, is expressed in pericytes of all organs. Here, we examined the role of CYGB in the development of pancreatic cancer. CYGB expression appeared predominately in the area surrounding adenocarcinoma and negatively correlated with tumor size in patients with pancreatic cancer. Directly injecting 7, 12-dimethylbenz[a]anthracene into the pancreatic tail in wild-type mice resulted in time-dependent induction of severe pancreatitis, fibrosis, and oxidative damage, which was rescued byCygboverexpression in transgenic mice. Pancreatic cancer incidence was 93% in wild-type mice but only 55% in transgenic mice. Enhanced CYGB expression in human pancreatic stellate cells in vitro reduced cellular collagen synthesis, inhibited cell activation, increased expression of antioxidant-related genes, and increased CYGB secretion into the medium.Cygb-overexpressing or recombinant human CYGB (rhCYGB) -treated MIA PaCa-2 cancer cells exhibited dose-dependent cell cycle arrest at the G1 phase, diminished cell migration, and reduction in colony formation. RNA sequencing in rhCYGB-treated MIA PaCa-2 cells revealed downregulation of cell cycle and oxidative phosphorylation pathways. An increase in MIA PaCa-2 cell proliferation and reactive oxygen species production by H2O2challenge was blocked by rhCYGB treatment orCygboverexpression. PANC-1, OCUP-A2, and BxPC-3 cancer cells showed similar responses to rhCYGB. Known antioxidants N-acetyl cysteine and glutathione also inhibited cancer cell growth. These results demonstrate that CYGB suppresses pancreatic stellate cell activation, pancreatic fibrosis, and tumor growth, suggesting its potential therapeutic application against pancreatic cancer.
COVID-19 vaccine development: milestones, lessons and prospects
Authors: Li Maochen , Wang Han , Tian Lili , Pang Zehan , Yang Qingkun , Huang Tianqi , Fan Junfen , Song Lihua , Tong Yigang , Fan Huahao 
Published: May 2022

AbstractWith the constantly mutating of SARS-CoV-2 and the emergence of Variants of Concern (VOC), the implementation of vaccination is critically important. Existing SARS-CoV-2 vaccines mainly include inactivated, live attenuated, viral vector, protein subunit, RNA, DNA, and virus-like particle (VLP) vaccines. Viral vector vaccines, protein subunit vaccines, and mRNA vaccines may induce additional cellular or humoral immune regulations, including Th cell responses and germinal center responses, and form relevant memory cells, greatly improving their efficiency. However, some viral vector or mRNA vaccines may be associated with complications like thrombocytopenia and myocarditis, raising concerns about the safety of these COVID-19 vaccines. Here, we systemically assess the safety and efficacy of COVID-19 vaccines, including the possible complications and different effects on pregnant women, the elderly, people with immune diseases and acquired immunodeficiency syndrome (AIDS), transplant recipients, and cancer patients. Based on the current analysis, governments and relevant agencies are recommended to continue to advance the vaccine immunization process. Simultaneously, special attention should be paid to the health status of the vaccines, timely treatment of complications, vaccine development, and ensuring the lives and health of patients. In addition, available measures such as mix-and-match vaccination, developing new vaccines like nanoparticle vaccines, and optimizing immune adjuvant to improve vaccine safety and efficacy could be considered.
Anti-cancer effect of LINC00478 in bladder cancer correlates with KDM1A-dependent MMP9 demethylation
Authors: Yang Han-Jie , Liu Tian , Xiong Yang 
Published: May 2022

AbstractAccumulating evidence has highlighted the important roles of long intergenic non-coding RNAs (lincRNAs) during cancer progression. However, the involvement of LINC00478 in bladder cancer remains largely unclear. Accordingly, the current study sought to investigate the function of LINC00478 on malignant phenotypes of bladder cancer cells as well as the underlying mechanism. By integrating data from in silico analysis, we uncovered that LINC00478 was differentially expressed in bladder cancer. We further analyzed the expression of LINC00478 and matrix metalloprotein 9 (MMP9) in bladder cancer tissues and cell lines and observed a significant decline in LINC00478 expression and an elevation in MMP9 expression. In addition, chromatin immunoprecipitation, RNA-binding protein immunoprecipitation, and RNA pull-down assays predicted and validated that LINC00478 targeted lysine-specific demethylase-1 (KDM1A) and down-regulated the expression of MMP9 by decreasing the monomethylation on lysine 4 of histone H3 (H3K4me1) of MMP9 promoter. Treatment with KDM1A inhibitor tranylcypromine (TCP) also led to an increase in the enrichment of H3K4me1 in the MMP9 promoter region. Through gain- and loss-of-function approaches, we found that LINC00478 up-regulation diminished the malignant phenotype of bladder cancer cells in vitro, and further inhibited xenograft tumor growth and metastasis in vivo by repressing MMP9. Collectively, our findings unraveled a LINC00478-mediated inhibitory mechanism in bladder cancer via the recruitment of histone demethylation transferase KDM1A to the MMP9 promoter region, which can provide potential implications for novel therapeutic targets against bladder cancer.
circDHTKD1 promotes lymphatic metastasis of bladder cancer by upregulating CXCL5
Authors: Lu Qun , Yin Haoli , Deng Yongming , Chen Wei , Diao Wenli , Ding Meng , Cao Wenmin , Fu Yao , Mo Wenjing , Chen Xiaoqing , Zhang Qing , Zhao Xiaozhi , Guo Hongqian 
Published: May 2022

AbstractLymph node (LN) metastasis is associated with unfavorable prognosis of bladder cancer (BCa). Although lymphangiogenesis is functionally important in LN metastasis of tumors, the potential mechanism in BCa remains unclear. Here, we clarified a regulatory mechanism of circRNA-mediated lymphangiogenesis and LN metastasis in BCa based on next-generation sequencing data. We revealed that circDHTKD1 was positively associated with LN metastasis and significantly upregulated in BCa. By analyzing the co-expression patterns of circDHTKD1 and differentially expressed mRNAs, we identified that circDHTKD1 facilitated lymphangiogenesis by upregulating CXCL5. Mechanistically, circDHTKD1 directly interacted with miR-149-5p, and antagonized the repression of miR-149-5p on CXCL5. Furthermore, circDHTKD1-induced CXCL5 expression recruited and activated neutrophils, which participated in lymphangiogenesis by secreting VEGF-C. Our study supports circDHTKD1 as a promising diagnostic and therapeutic target for LN metastasis in BCa.
Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis
Authors: Yu Fan , Zhang Qianping , Liu Hanyu , Liu Jinming , Yang Song , Luo Xiaofan , Liu Wei , Zheng Hao , Liu Qiqi , Cui Yunxi , Chen Guo , Li Yanjun , Huang Xinglu , Yan Xiyun , Zhou Jun , Chen Quan 
Published: May 2022

AbstractFerroptosis is a regulated iron-dependent cell death characterized by the accumulation of lipid peroxidation. A myriad of facets linking amino acid, lipid, redox, and iron metabolisms were found to drive or to suppress the execution of ferroptosis. However, how the cells decipher the diverse pro-ferroptotic stress to activate ferroptosis remains elusive. Here, we report that protein O-GlcNAcylation, the primary nutrient sensor of glucose flux, orchestrates both ferritinophagy and mitophagy for ferroptosis. Following the treatment of ferroptosis stimuli such as RSL3, a commonly used ferroptosis inducer, there exists a biphasic change of protein O-GlcNAcylation to modulate ferroptosis. Pharmacological or genetic inhibition of O-GlcNAcylation promoted ferritinophagy, resulting in the accumulation of labile iron towards mitochondria. Inhibition of O-GlcNAcylation resulted in mitochondria fragmentation and enhanced mitophagy, providing an additional source of labile iron and rendering the cell more sensitive to ferroptosis. Mechanistically, we found that de-O-GlcNAcylation of the ferritin heavy chain at S179 promoted its interaction with NCOA4, the ferritinophagy receptor, thereby accumulating labile iron for ferroptosis. Our findings reveal a previously uncharacterized link of dynamic O-GlcNAcylation with iron metabolism and decision-making for ferroptosis, thus offering potential therapeutic intervention for fighting disease.
DNA methylation signature of chronic low-grade inflammation and its role in cardio-respiratory diseases
Authors: Wielscher Matthias , Mandaviya Pooja R. , Kuehnel Brigitte , Joehanes Roby , Mustafa Rima , Robinson Oliver , Zhang Yan , Bodinier Barbara , Walton Esther , Mishra Pashupati P. , Schlosser Pascal , Wilson Rory , Tsai Pei-Chien , Palaniswamy Saranya , Marioni Riccardo E. , Fiorito Giovanni , Cugliari Giovanni , Karhunen Ville , Ghanbari Mohsen , Psaty Bruce M. , Loh Marie , Bis Joshua C. , Lehne Benjamin , Sotoodehnia Nona , Deary Ian J. , Chadeau-Hyam Marc , Brody Jennifer A. , Cardona Alexia , Selvin Elizabeth , Smith Alicia K. , Miller Andrew H. , Torres Mylin A. , Marouli Eirini , Gào Xin , van Meurs Joyce B. J. , Graf-Schindler Johanna , Rathmann Wolfgang , Koenig Wolfgang , Peters Annette , Weninger Wolfgang , Farlik Matthias , Zhang Tao , Chen Wei , Xia Yujing , Teumer Alexander , Nauck Matthias , Grabe Hans J. , Doerr Macus , Lehtimäki Terho , Guan Weihua , Milani Lili , Tanaka Toshiko , Fisher Krista , Waite Lindsay L. , Kasela Silva , Vineis Paolo , Verweij Niek , van der Harst Pim , Iacoviello Licia , Sacerdote Carlotta , Panico Salvatore , Krogh Vittorio , Tumino Rosario , Tzala Evangelia , Matullo Giuseppe , Hurme Mikko A. , Raitakari Olli T. , Colicino Elena , Baccarelli Andrea A. , Kähönen Mika , Herzig Karl-Heinz , Li Shengxu ,   BIOS consortium, Conneely Karen N. , Kooner Jaspal S. , Köttgen Anna , Heijmans Bastiaan T. , Deloukas Panos , Relton Caroline , Ong Ken K. , Bell Jordana T. , Boerwinkle Eric , Elliott Paul , Brenner Hermann , Beekman Marian , Levy Daniel , Waldenberger Melanie , Chambers John C. , Dehghan Abbas , Järvelin Marjo-Riitta 
Published: May 2022

AbstractWe performed a multi-ethnic Epigenome Wide Association study on 22,774 individuals to describe the DNA methylation signature of chronic low-grade inflammation as measured by C-Reactive protein (CRP). We find 1,511 independent differentially methylated loci associated with CRP. These CpG sites show correlation structures across chromosomes, and are primarily situated in euchromatin, depleted in CpG islands. These genomic loci are predominantly situated in transcription factor binding sites and genomic enhancer regions. Mendelian randomization analysis suggests altered CpG methylation is a consequence of increased blood CRP levels. Mediation analysis reveals obesity and smoking as important underlying driving factors for changed CpG methylation. Finally, we find that an activated CpG signature significantly increases the risk for cardiometabolic diseases and COPD.
Indigenous oyster fisheries persisted for millennia and should inform future management
Authors: Reeder-Myers Leslie , Braje Todd J. , Hofman Courtney A. , Elliott Smith Emma A. , Garland Carey J. , Grone Michael , Hadden Carla S. , Hatch Marco , Hunt Turner , Kelley Alice , LeFebvre Michelle J. , Lockman Michael , McKechnie Iain , McNiven Ian J. , Newsom Bonnie , Pluckhahn Thomas , Sanchez Gabriel , Schwadron Margo , Smith Karen Y. , Smith Tam , Spiess Arthur , Tayac Gabrielle , Thompson Victor D. , Vollman Taylor , Weitzel Elic M. , Rick Torben C. 
Published: May 2022

AbstractHistorical ecology has revolutionized our understanding of fisheries and cultural landscapes, demonstrating the value of historical data for evaluating the past, present, and future of Earth’s ecosystems. Despite several important studies, Indigenous fisheries generally receive less attention from scholars and managers than the 17th–20th century capitalist commercial fisheries that decimated many keystone species, including oysters. We investigate Indigenous oyster harvest through time in North America and Australia, placing these data in the context of sea level histories and historical catch records. Indigenous oyster fisheries were pervasive across space and through time, persisting for 5000–10,000 years or more. Oysters were likely managed and sometimes “farmed,” and are woven into broader cultural, ritual, and social traditions. Effective stewardship of oyster reefs and other marine fisheries around the world must center Indigenous histories and include Indigenous community members to co-develop more inclusive, just, and successful strategies for restoration, harvest, and management.