bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative miRNA Transcriptomics of Mouse and Macaque Reveals MYOC is An Inhibitor for C. neoformans Invasion into Brain
Authors: Li Hailong , Han Xiaoxu , Du Wei , Meng Yang , Li Yanjian , Sun Tianshu , Liang Qiaojing , Li Chao , Suo Chenhao , Gao Xindi , Qiu Yu , Tian Wen , An Minghui , Zhang Hui , Fu Yajing , Li Xiaolin , Lan Tian , Yang Sheng , Zhang Zining , Geng Wenqing , Ding Chen , Shang Hong 
Published: February 2022

AbstractCryptococcal meningoencephalitis is an emerging infection shifted from primarily ART-naive to being ART-experienced HIV/AIDS patients, COVID-19 patients and also in immune competent individuals, mainly caused by the human opportunistic pathogen Cryptococcus neoformans, yet mechanisms of the brain or CNS dissemination remain to elucidate, which is the deadest process for the disease. Meanwhile, illustrations of clinically relevant responses in cryptococcosis were limited, as the low availabilities of clinical samples. In this study, macaque and mouse infection models were employed and miRNA-mRNA transcriptomes were performed and combined, which revealed cytoskeleton, a major feather in HIV/AIDS patients, was a centric pathway regulated in both two infection models. Notably, assays of clinical immune cells confirmed an enhanced “Trojan Horse” in HIV/AIDS patients, which can be shut down by cytoskeleton inhibitors. Furthermore, we identified a novel enhancer for macrophage “Trojan Horse”, myocilin, and an enhanced fungal burden was achieved in brains of MYOC transgenic mice. Taking together, this study reveals fundamental roles of cytoskeleton and MYOC in blocking fungal CNS dissemination, which not only helps to understand the high prevalence of cryptococcal meningitis in HIV/AIDS, but also facilitates the development of novel drugs for therapies of meningoencephalitis caused by C. neoformans and other pathogenic microorganisms.
Optical Control of Membrane Fluidity Modulates Protein Secretion
Authors: Jiménez-Rojo Noemi , Feng Suihan , Morstein Johannes , Pritzl Stefanie D. , Harayama Takeshi , Asaro Antonino , Vepřek Nynke A. , Arp Christopher J. , Reynders Martin , Novak Alexander J. E. , Kanshin Evgeny , Ueberheide Beatrix , Lohmüller Theobald , Riezman Howard , Trauner Dirk 
Published: February 2022

AbstractThe lipid composition of cellular membranes is dynamic and undergoes remodelling affecting biophysical properties, such as membrane fluidity, which are critical to biological function. Here, we introduce an optical approach to manipulate membrane fluidity based on exogenous synthetic fatty acid with an azobenzene photoswitch, termed FAAzo4. Cells rapidly incorporate FAAzo4 into phosphatidylcholine (PC), the major phospholipid in mammalian cells, in a concentration- and cell type-dependent manner. This generates photoswitchable PC analogs (AzoPC), which are predominantly located in the endoplasmic reticulum (ER). Irradiation causes a rapid photoisomerization that increases membrane fluidity with high spatiotemporal precision. We use these ‘PhotoCells’ to study the impact of membrane mechanics on protein export from the ER and demonstrate that this two-step process has distinct membrane fluidity requirements. Our approach represents an unprecedented way of manipulating membrane fluidity in cellulo and opens novel avenues to probe roles of fluidity in a wide variety of biological processes.
Molecular dynamics simulations of the Spike trimeric ectodomain of the SARS-CoV-2 Omicron variant: structural relationships with infectivity, evasion to immune system and transmissibility
Authors: Amorim Vitor Martins de Freitas , de Souza Robson Francisco , Souza Anacleto Silva de , Guzzo Cristiane Rodrigues 
Published: February 2022

AbstractThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant is replacing Delta, the most prevalent variant worldwide from the beginning of 2021 until early 2022. The Omicron variant is highly transmissible and responsible for a new worldwide COVID-19 wave. Herein, we calculated molecular dynamics simulations of the SARS-CoV-2 trimeric spike protein of Wuhan-Hu-1 strain (wild type, WT) and the Omicron variant of concern. Structural analyses reveal that the SpikeOmicron presents more conformational flexibility than SpikeWT, mainly in the N-terminal domain (NTD) and receptor-binding domain (RBD). Such flexibility results in a broader spectrum of different conformations for SpikeOmicron, whereby the RBD can more easily visit an up-conformational state. We reported how the mutations in this variant may influence the intra- and inter-protomer contacts caused by conformational flexibility of the NTD. Based on our analysis, we suggest that the differences in conformational flexibility between SpikeOmicron and SpikeWT may explain the observed gains in infectivity, immune system evasion and transmissibility in this novel variant.Graphical abstract
Vector competence and transcriptional response of Aedes aegypti for Ebinur Lake virus, a newly mosquito-borne orthobunyavirus
Authors: Yang Cihan , Wang Fei , Huang Doudou , Ma Haixia , Zhao Lu , Zhang Guilin , Li Hailong , Han Qian , Bente Dennis , Yuan Zhiming , Xia Han 
Published: February 2022

AbstractThe global impact of mosquito-borne diseases is increasing in the last decades. The newly classified orthobunyavirus, Ebinur Lake virus (EBIV) has been verified with highly virulent pathogenic to adult laboratory mice, and antibodies against EBIV have been detected in humans. As a potential emerging virus, it is necessary to assess the vector capacity of mosquitoes for EBIV to predicting its risk to public health. Herein, Aedes aegypti, the gradually important vector in China, was used as a model to evaluate the vector competence for EBIV. It was showed that EBIV can be transmitted by Ae. aegypti through oral feeding and the transmission rates could get to 11.8% at 14 days post infection (dpi). The highest infection rate, dissemination rate and ovary infection rate were 70%, 42.9%, and 29.4%, respectively. Through intrathoracic infection, Ae. aegypti was highly susceptible to EBIV and the transmission rates could get to 90% at 10 dpi. Moreover, the infection rate, dissemination rate and ovary infection rate were all 100%. Transcriptome analysis demonstrated EBIV can alter expressions of mosquito genes related to immune-related process and metabolism-related process. Defensin-C and chitinase 10 had been continuously down-regulated in the mosquitoes infected by intrathoracic inoculation. Our studies made the comprehensive analysis of the vector competence and transcriptional response of Ae. aegypti for EBIV, which implied the potential risk of EBIV to public health. Moreover, these findings indicated a complex interplay between EBIV and the mosquito immune system to affect the vector transmission capability.
Oligodendrocyte origin and development in the zebrafish visual system
Authors: Santos-Ledo Adrián , Montes-Perez Cristina , DeOliveira-Mello Laura , Arévalo Rosario , Velasco Almudena 
Published: February 2022

AbstractOligodendrocytes are the myelinating cells in the central nervous system (CNS). Their developmental origin and specification are well known in birds and mammals but remains unclear in fish. To fulfill this gap, we have studied their early progression during zebrafish visual morphogenesis using the transgenic line Olig2:GFP. We have tracked the Olig2+ cells in the visual system from 48 hours post fertilization (hpf) until 11 days post fertilization (dpf). We have also analyzed the differential expression of the Sox2 and Sox10 transcription factors in this cellular line. Oligodendrocyte progenitor cells (OPCs) originate at 48hpf in regions close to the preoptic area, near to the ventral hypothalamus. Then, at 5 dpf, they migrate to the optic chiasm, where they invade the optic nerve, extending towards the retina. While OPCs in the retina also express Sox2, in the the optic tectum they express Sox10. Within the optic nerve tract, they express both. We have also observed that these Olig2:GFP line do not colocalize with the expression of Mbpa, a myelin marker, but are intimately intertwined. Our data matches with other animal models, where OPCs are specified in the preoptic area and migrate to the optic nerve through the optic chiasm. We revealed that oligodendrocyte is a complex population of cells expressing different transcription factors.
Genetic load in incomplete lupus erythematosus
Authors: Slief Matt , Kheir Joseph , Smith Miles , Mowery Colin , Macwana Susan , DeJager Wade , Wagner Catriona A. , Aberle Teresa , James Judith A. , Guthridge Joel M. 
Published: February 2022

ABSTRACTIncomplete lupus erythematosus (ILE) patients have lupus features but insufficient criteria for systemic lupus erythematosus (SLE) classification. Some ILE patients transition to SLE, but most avoid major organ involvement. This study tested whether the milder disease course in ILE is influenced by reduced SLE-risk allele genetic load. We calculated the genetic load based on 99 SLE-associated risk alleles in European American SLE patients (>4 ACR-1997 criteria, n=171), ILE patients (3 ACR-1997 criteria; n=174), a subset of ILE patients not meeting SLICC classification (ILESLICC, n=119), and healthy controls (n=133). ILE and SLE patients had significantly greater SLE-risk allele genetic load compared to healthy controls, while ILESLICC patients had a trend toward an increased genetic load, although not statistically significant. However, the genetic load did not differ between ILE and SLE. In conclusion, ILE and SLE patients have comparable genetic loads of SLE risk loci, suggesting similar genetic predisposition between these conditions. Phenotypic differences between SLE and ILE may instead be influenced by ILE-specific variants and gene-environment interactions.
Octopaminergic/tyraminergicTdc2neurons regulate biased sperm usage in femaleDrosophila melanogaster
Authors: Chen Dawn S. , Clark Andrew G. , Wolfner Mariana F. 
Published: February 2022

AbstractIn polyandrous internally fertilizing species, a multiply-mated female can use stored sperm from different males in a biased manner to fertilize her eggs. The female’s ability to assess sperm quality and compatibility is essential for her reproductive success, and represents an important aspect of postcopulatory sexual selection. InDrosophila melanogaster, previous studies demonstrated that the female nervous system plays an active role in influencing progeny paternity proportion, and suggested a role for octopaminergic/tyraminergicTdc2neurons in this process. Here, we report that inhibitingTdc2neuronal activity causes females to produce a higher-than-normal proportion of first-male progeny. This difference is not due to differences in sperm storage or release, but instead is attributable to the suppression of second-male sperm usage bias that normally occurs in control females. We further show that a subset ofTdc2neurons innervating the female reproductive tract is largely responsible for the progeny proportion phenotype that is observed whenTdc2neurons are inhibited globally. On the contrary, overactivation ofTdc2neurons does not further affect sperm storage and release or progeny proportion. These results suggest that octopaminergic/tyraminergic signaling allows a multiply-mated female to bias sperm usage, and identify a new role for the female nervous system in postcopulatory sexual selection.
Functional characterization of a neuropeptide receptor exogenously expressed in Aplysia neurons
Authors: Zhang Guo , Guo Shi-Qi , Yin Si-Yuan , Yuan Wang-Ding , Chen Ping , Kim Ji-Il , Wang Hui-Ying , Zhou Hai-Bo , Susswein Abraham J. , Kaang Bong-Kiun , Jing Jian 
Published: February 2022

AbstractNeuropeptides act mostly on a class of G-protein coupled receptors, and play a fundamental role in the functions of neural circuits underlying behaviors. However, the functions of neuropeptide receptors are poorly understood. Here, we used the mollusc model system Aplysia and microinjected the exogenous neuropeptide receptor apATRPR (Aplysia Allatotropin-related peptide receptor) with an expression vector (pNEX3) into Aplysia neurons that did not express the receptor. Physiological experiments demonstrated that apATRPR could mediate the excitability increase activated by its ligand, apATRP (Aplysia Allatotropin-related peptide), in the Aplysia neurons that now express the receptor. This study provides the first definitive evidence for a physiological function of a neuropeptide receptor in molluscan animals.
A novel dual-responsive halloysite nano-container for anti-corrosion coatings
Authors: Liu Yuanwei , Wang Bin , Xie Yan , Chen Yu , Yang Zhongnian , Han Guojun , Dang Yanqiu 
Published: February 2022

Purpose
The purpose of this paper is to prepare a dual-encapsulated halloysite nano-container to release the capsuled inhibitor as an additive for corrosion protection of epoxy coating.

Design/methodology/approach
Halloysite nano-containers (HNT) were prepared by simultaneously implanting inhibitor benzotriazole (BTA) into the inside and outside of the halloysite using reduced pressure and layer-by-layer (LBL) assembly, respectively. The microstructure and morphology of treated HNT were investigated using Fourier transform infrared spectroscopy and transmission electron microscopy. In addition, the anti-corrosion behaviors of the composite polyepoxy coating with inhibitor-loaded nano-containers BTA@HNT-2 were investigated using the electrochemical impedance spectroscopy and neutral salt spray test.

Findings
Test results showed that the LBL assembly structure of the halloysite nano-container makes the BTA@HNT-2 nano-container be controlled and sustained to release BTA, relying on the pH. Very importantly, the obtained nano-container is also responsive to temperature, owing to the thermosensitivity polyelectrolyte out-shell of the HNT. The result showed Rct of the composite polyepoxy coating can be sufficient to maintain higher than 8.510E+7 Ω·cm2 over 72 h of immersion test. Moreover, the artificial induced defects on the coating surface were sufficiently inhibited in the presence of BTA@HNT-2 nano-container in the polyepoxy coating.

Originality/value
Use of the BTA@HNT-2 as corrosion inhibitor nano-container, with good anti-corrosion property and dual-responsive to pH and temperature, offers a significant rout to prepare smart anti-corrosion coating for protecting metal substrate.
Acceptability and preliminary effectiveness of a text messaging intervention to reduce high-risk alcohol use among adults 50 and older: an exploratory study
Authors: Kuerbis Alexis , Behrendt Silke , Arora Varnica , Muench Frederick J. 
Published: February 2022

Purpose
Prevalence rates of older adults (OA, 50+) with high-risk alcohol use are rapidly growing. Barriers to intervention persist for OA. Mobile interventions, specifically short message service (SMS, also known as text messaging), provide an opportunity to overcome such barriers. The purpose of this paper is to explore the acceptability and preliminary effectiveness of four SMS intervention conditions compared to mobile assessment only among OA (50–65 years old, n = 49) who drink at high-risk levels compared to younger adults (YA, 21–49 years old, n = 102).

Design/methodology/approach
Within and between age group differences at baseline and end-of-treatment (12 weeks) were explored. Generalized linear modeling was used to test age by receipt of any SMS intervention (compared to assessment only) effects across health outcomes. Effect sizes and visual analyses were generated to assess for age by type of SMS interactions.

Findings
Both age groups reported intervention satisfaction. Two-thirds elected to continue receiving SMSs after treatment concluded. Descriptively, SMS groups demonstrated reduced drinking across age groups at end-of-treatment compared to mobile assessment only, though slightly larger effects were found for YA than for OA. Age significantly moderated the receipt of any SMS intervention only for depression (i.e., older age weakened the therapeutic effect). Of all the SMS types, tailored messages outperformed the other conditions on drinking outcomes for both age groups. Depression, craving and alcohol problems responded to gain-framed messages among OA and loss-framed messages among YA.

Research limitations/implications
The findings of this paper suggest that SMS is acceptable and potentially effective across age groups. Further adaptation of SMS across age groups could enhance its effectiveness.

Originality/value
This is the first paper to explore the impact of older age on the efficacy of text messaging as an intervention for hazardous alcohol use.
The differential impact of monological and dialogical corporate social responsibility communication strategies on brand trust in the credence goods market
Authors: Dai Wenbin , Reich Allen Z. 
Published: February 2022

PurposeThis study aims at investigating the differential impact of different CSR communication strategies on consumers' brand trust and consequent attitudes and behaviors in the credence goods market.Design/methodology/approachAn experimental laboratory study was conducted to collect data from graduate students (n = 414) from two Chinese universities. Structural equation modeling was used to test the hypothesized model.FindingsThe results show that a dialogical communication strategy, compared to a monological communication strategy, is significantly more effective in increasing consumers' brand trust for credence goods as well as their willingness-to-buy and positive word-of-mouth. The results also confirm the mediating effect of CSR knowledge and the moderating effect of broad-scope trust on the relationship between CSR communication strategies and brand trust.Practical implicationsThis study implies that in order to promote consumer trust and supportive behaviors toward brands, managers of companies in credence goods market should consider the increased use of various dialogical CSR communication strategies.Originality/valueThe study is one of the first to verify the impact of dialogical vs monological CSR communication strategies on consumers of credence goods.
Pre- and post-production water treatment in the food processing industry: managerial perceptions of environmental pressure increase adoption of voluntary environmental management
Authors: Meng Ting , Jiang Qijun , Florkowski Wojciech J. 
Published: February 2022

PurposeThis paper examines pre- and post-production water treatment practices among food processors and investigates factors, especially managerial perceptions of environmental pressure that encourage or preclude either process.Design/methodology/approachTo consider potential spillover effects across two water-treatment practices, the bivariate probit model based on random utility theory is used to investigate how practices are influenced by managerial perceptions of environmental pressure and measured by manager perceptions on water costs, water availability, water safety and quality.FindingsResults indicate that firms with a managerial perception that water costs are low are less likely to conduct both pre- and post-production water treatment practices, while the perception of high water quality has a negative effect on water treatment prior to use. This study also confirms the positive correlation of the pre- and post-water treatment practices among food processors. Practices also change with firm features including production scope, scale, target market and expected future sales growth.Practical implicationsThis study provides unique insights about water treatment practices and generates knowledge to enhance food safety and environmental sanitation in the food industry. Results are helpful to design and provide additional training and educational programs that target the enhancement of environmental and water quality awareness among food company managers and modify food safety policy instruments and environmental regulations pertaining to surface water resources.Originality/valueResearch exploring water-treatment practices in the food industry has been limited. Using a representative sample of food processors in the city of Shanghai, this study contributes to the literature on the examination of internal drivers of voluntary environmental management (VEM) with a focus on managerial perceptions of environmental pressure, establishes the correlation between pre- and post-production water treatment practices and identifies and quantifies the effects of relevant factors.
Probabilistic weighted voting model using multiple machine learning methods for fault detection and classification
Authors: Ülker Fevzeddin , Küçüker Ahmet 
Published: February 2022

Purpose
The individual machine learning methods used for fault detection and classification have accuracy performance at a certain level. A combined learning model composed of different base classifiers rather than an individual machine learning model is introduced to ensure diversity. In this way, this study aims to improve the generalization capability of fault detection and classification scheme.

Design/methodology/approach
This study presents a probabilistic weighted voting model (PWVM) with multiple learning models for fault detection and classification. The working principle of this study’s proposed model relies on weight selection and per-class possibilities corresponding to predictions of base classifiers. Moreover, it can improve the power of the prediction model and cope with imbalanced class distribution through validation metrics and F-score.

Findings
The performance of the proposed PWVM was better than the performance of the individual machine learning methods. Besides, the proposed voting model’s performance was compared with different voting mechanisms involving weighted and unweighted voting models. It can be seen from the results that the presented model is superior to voting mechanisms. The performance results revealed PWVM has a powerful predictive model even in noisy conditions. This study determines the optimal model from among voting models with the prioritization method on data sets partitioned different ratios. The obtained results with statistical analysis verified the validity of the proposed model. Besides, the comparative results from different benchmark data sets verified the effectiveness and robustness of this study’s proposed model.

Originality/value
The contribution of this study is that PWVM is an ensemble model with outstanding generalization capability. To the best of the authors’ knowledge, no study has been performed using a PWVM composed of multiple classifiers to detect no-faulted/faulted cases and classify faulted phases.