bluuebunny/crossref_metadata_2025_split huggingface dataset:
Severe Heart Failure and Outcomes in 121 Children With Dilated Cardiomyopathy: A Single-Center Retrospective Study from Southwest China
Authors: Chen Cheng , Su Danyan , Qin Suyuan , Ye Bingbing , Huang Yanyun , Zhao Weiying , Huang Yuqin , Liu Dongli , Pang Yusheng 
Published: February 2022

Abstract
Objective
Heart failure is a common complication in children with dilated cardiomyopathy. The aim of this study was to determine whether severe heart failure at diagnosis was related to poor outcomes in children with dilated cardiomyopathy.
Methods
We analyzed medical data from 121 children with dilated cardiomyopathy in our hospital from 2003 to 2021. The children were grouped by the degree of heart failure. Cox regression analysis was performed to confirm whether severe heart failure was associated with poor outcomes.
Results
There were 121 patients with dilated cardiomyopathy in this research. The mean age of the 121 patients was 118.5 ± 63.0 months. Of these patients, 81 (67.8%) were sorted into a severe heart failure group and 39 (32.2%) were placed into a mild heart failure group. A comparison between the two groups showed that patients with severe heart failure were older and more likely to be male, have lower systolic blood pressure measurements, be admitted to the intensive care unit and be treated with β-blockers, loop diuretics, spironolactone and digoxin. In addition, they had larger systolic and diastolic left ventricular end-diastolic dimensional and lower left ventricular ejection fractional z-scores as well as being more prone to aortic and tricuspid regurgitations (P < 0.05 in all cases). After adjusting for age, sex, heart rate, left ventricular ejection fraction z-scores and drug, severe heart failure was found to be independently associated with an increased risk of death (hazard ratio [HR] 2.27; P < 0.005).
Conclusions
Severe heart failure at diagnosis was related to a greater risk of death in children with dilated cardiomyopathy. Further prospective studies are warranted to evaluate the efficacy between the treatment undertaken for heart failure and outcomes in children with dilated cardiomyopathy.
The Role Of Single-Cell RNA Sequencing In Cardiac Tumor - A Case Report
Authors: Qi Xianjie , Shi Chao , Wei Ting , Zhang Shengqiang , Meng Jinjin , Jiang Yiyao 
Published: February 2022

Abstract
Background: The benign cardiac tumor is a common disease. We often completely removed it by operation. However, the characteristic of the tumor is not studied deeply. Here, we used the technique of single-cell RNA sequencing to analyze cell composition and growth patterns in a giant cardiac tumor. Case presentation: A 65-year-old woman presented to our hospital with 5 day of chest tightness, dyspnea, lower abdominal distension. Echocardiography revealed a mass in the right atrium. An emergency operation was carried out to prevent tumor shedding. The single-cell RNA sequencing was applied to analysis the nature of the tumor.Conclusion: Single-cell RNA sequencing is a good manner to analyze the growth pattern and search for therapeutic targets. Based on the result of single-cell RNA sequencing, it is helpful to reduce the recurrence of the tumor and benefit the quality of life of patients.
Application of vancomycin-impregnated calcium sulfate hemihydrate/nano- hydroxyapatite/chitosan injectable hydrogels combined with BMSCs sheet for the treatment of infected bone defects in rabbit model
Authors: Wang Yanjun , Zhao Zihou , Liu Shiyu , Luo Wen , Wang Guoliang , Zhu Zhenfeng , Ma Qiong , Liu Yunyan , Wang Linhu , Lu Shuaikun , Zhang Yong , Qian Jixian , Zhang Yunfei 
Published: February 2022

Abstract
Background
Choice of bone substitutes for the therapy of infected bone defects (IBDs) attracts surgeons’ attention for years. However, single-stage bioabsorbable material that provides carriers for antibiotic releasing as well as scaffold for BMSCs sheet need further exploration. Our study was designed to investigate the effect of vancomycin-loaded calcium sulfate hemihydrate/nano-hydroxyapatite/chitosan (CSH/n-HA/CTS) hydrogels combined with BMSCs sheet as bone substitute in the treatment of IBDs.
Methods
The BMSCs were harvested and cultured into cell sheet. After successful establishment of animal model with chronic osteomyelitis, the 48 New Zealand white rabbits were randomly divided into 4 groups. Animals in group A were treated with thorough debridement as control. Group B were managed with BMSCs sheet. CSH/n-HA/CTS hydrogels were implanted in group C, and group D were treated with CSH/n-HA/CTS + BMSCs sheet. Gross observation and 3D reconstruction of Micro CT were performed to assess the osteogenic and infection elimination ability. Histological staining (Hematoxylin and eosin and Van Gieson) was used to observe inflammatory cell infiltration and formation of collagen fiber 4, 8 ,12 weeks after implantation.
Results
The bone defect of the control group was not repaired in 12 weeks as chronic osteomyelitis still existed. HE staining showed a large number of inflammatory cell infiltration around the tissue, and VG staining showed no new collagen fiber formation. In the group of BMSCs sheet, though new bone formation was observed by gross observation and Micro-CT scan, the infection was not effectively controlled due to the unfilled cavity. Some neutrophils and only a small amount of collagen fiber could be observed. Both groups of hydrogels and hydrogels/BMSCs achieved satisfactory repair effect and infection control. 3D reconstruction of Micro-CT at 4 weeks showed that the hydrogels/BMSCs sheet group had higher reconstruction efficiency and better bone modeling with normal morphology. HE staining showed little aggregation of inflammatory cells, and VG staining showed a large number of new collagen fibers.
Conclusions
Our preliminary results proved that compared with single material, the novel antibiotic-impregnated hydrogels acted as superior scaffold for BMSCs sheet and excellent antibiotics vector against infection, which provided a basis for applying tissue engineering technology to the treatment of chronic osteomyelitis.
Acute upper gastrointestinal hemorrhage with acute pulmonary embolism: A difficult decision?
Authors: Li Che , Chu Zhenliang , Zhang Bing , Zheng Jifeng 
Published: February 2022

Abstract
Upper gastrointestinal hemorrhage and pulmonary embolism are potentially life-threatening conditions with high morbidity and mortality, However, acute upper gastrointestinal hemorrhage with acute pulmonary embolism (APE) is rare, and whether anti-coagulation is a difficult choice for doctors. We report the case of a 70-year-old man with a 3-day history of abdominal pain accompanied by a black stool, He was admitted to our department, then, he was diagnosed with gastric ulcers, and he suffered from a near-syncopal episode accompanied by heavy sweating. Computed tomography pulmonary angiography (CTPA) revealed a partial embolism in the two side pulmonary arteries. Anticoagulant treatment was performed after a complete hematologic assessment for 20 days. The patient improved and was discharged home on warfarin anticoagulation for 3 months without any complications. For patients with a high bleeding risk of pulmonary embolism, clinicians should evaluate adequately before deciding to take anticoagulant therapy.
Photo-crosslinked gelatin methacryloyl strengthened with nanoparticles for regeneration of rabbit calvarial defects
Authors: Lee Dana , Park Jin-Young , Seo Young-Wook , Jin Xiang , Hong Jongmin , Bhattacharyya Amitava , Noh Insup , Choi Seong-Ho 
Published: February 2022

Abstract
Background
Gelatin methacryloyl (GelMa) hydrogel has been regarded as a promising biomaterial in the field of bioprinting and biofabrication. The aim of this study was to investigate the efficacy of photo-crosslinked GelMa hydrogel and calcium phosphate nanoparticles (CNp) when applying different fabrication methods for bone regeneration.
Methods
Four circular defects were created in the calvaria of 10 rabbits. Each defect was randomly allocated to the following study groups: 1) the sham control group; 2) the GelMa group (defect filled with crosslinked GelMa hydrogel); 3) the CNp-GelMa group (GelMa hydrogel crosslinked with nanoparticles); 4) CNp + GelMa group (crosslinked GelMa loaded with nanoparticles). At two, four and eight weeks, samples were harvested, and histological and micro-computed tomography analyses were performed.
Results
Histomorphometric analysis showed that CNp-GelMa and CNp + GelMa groups at 2 weeks were significantly greater in total augmented area compared to the control group (P < 0.05). Greatest new bone area was observed in the CNp-GelMa group, however this was without statistical significance (P > 0.05). Crosslinked GelMa hydrogel with nanoparticles exhibited good biocompatibility with minimal inflammatory reaction.
Conclusion
Crosslinked GelMa hydrogel with calcium phosphate nanoparticles might enhance new bone formation. Further improvement in mechanical stability and resistance to enzymatic degradation is needed prior to application in bone regenerative procedures.
Polyostotic fibrous dysplasia complicated by pathological fracture of right femoral shaft with nonunion-A Case Report
Authors: Dong Zhu , Qifan Yang , jing Liu , lei Tan , ye Jiang 
Published: February 2022

Abstract
Introduction: Fibrous dysplasia is a benign fibrous bone tumor that accounts for 5% to 10% of benign bone tumors. It can manifest as simple fibrous dysplasia (70% - 80%), polyostotic fibrous dysplasia (20% - 30%), with approximately the same incidence in men and women(1). We report a patient with a rare case of multiple fibrous dysplasia combined with proximal femoral shepherd deformity with pathological fracture of the femoral shaft complicated by nonunion. It is necessary to understand the disease in more detail to avoid overtreatment of benign lesions or misdiagnosis of malignant tumors and other diseases.Case presentation: A 58-year-old man with polyostotic fibrous dysplasia, bilateral proximal femur deformity, Shepherd's angle deformity, right femoral shaft pathological fracture complicated by nonunion, we under fluoroscopy, in the obvious proximal fracture, take osteotomy, and process the shape of the cut bone fragment to adapt it to the corrected force line, and then restore it back to its original position, using intramedullary nailing technology complete the operation.Three months after the operation, he came to the hospital for re-examination, and an X-ray of the right femur was taken. It was found that the fractured end had a tendency to heal. The patient was instructed to gradually bear weight. After six months of re-examination, the patient could walk with a walker. One year after the operation, the patient could walk without a walker and take care of himself at home. However, there was still stretch-like pain in the right lower back, but it was tolerable.Conclusions: For patients with polyostotic fibrous dysplasia combined with proximal femoral shepherd deformity and pathological fracture of the femoral shaft with nonunion, osteotomy combined with intramedullary nailing is a simple and convenient way to correct the deformity and obtain correct fracture alignment.
Muscle Size, Strength, and Physical Function in Response to Augmented Calorie Delivery: A TARGET Sub-Study
Authors: Chapple Lee-anne S , Summers Matthew J , Weinel Luke M , Lange Kylie , Yang Woo Han , Deane Adam M , Chapman Marianne J 
Published: February 2022

Abstract
Background
Critical illness leads to muscle wasting which may be attenuated with augmented calorie delivery. The Augmented versus Routine approach to Giving Energy Trial (TARGET) randomized 4000 patients to receive energy-dense (1.5kcal/ml) or standard (1.0kcal/ml) enteral nutrition. The aim of this sub-study was to evaluate whether augmented calorie delivery attenuates muscle loss and maintains strength and physical function when compared to routine care.
Methods
TARGET participants from a single participating ICU were eligible for enrolment in this sub-study if consent could be obtained. Ultrasound-derived muscle layer thickness (MLT) at three landmarks (quadriceps, forearm and mid-upper arm) and handgrip strength were measured at baseline and every 7 days until hospital discharge and at 3- and 6-months following randomization. Physical function was also assessed at 3- and 6-months using the ‘get up and go’ and 6-minute walk tests. Data are presented as mean ± standard deviation.
Results
Eighty patients (1.5kcal: n = 38, 58 ± 14 years, 60% male, APACHE II 20 ± 7 vs. 1.0kcal: n = 42, 54 ± 18 years, 66% male, APACHE II 22 ± 10) were recruited. Although patients in the 1.5kcal group received more calories (2075 ± 344 vs 1325 ± 313 kcal/day; P < 0.001), there was no significant difference in quadriceps MLT at any timepoint, including ICU discharge (primary outcome) (1.5kcal: 2.90 ± 1.27 vs 1.0kcal: 2.39 ± 1.06 cm; P = 0.141), hospital discharge (2.47 ± 1.03 vs 2.10 ± 1.08 cm; P = 0.227) or at 3- and 6-months. Similar relationships were seen for forearm and mid-upper arm MLT and handgrip strength at all timepoints. Patients in the 1.5 kcal group took less time to complete the ‘get up and go’ test (6.66 ± 1.33 vs. 9.11 ± 2.94 secs; mean group difference (95% CI) -2.45 (-4.35, -0.55); P = 0.014), but there was no difference between groups at the 6-month follow-up. There were no differences in the distances walked in the 6-minute walk test at either 3- or 6-month timepoints.
Conclusion
Augmented calorie delivery compared to routine care did not attenuate loss of muscle size or strength during hospital admission or at 3- and 6-months following randomization. Patients receiving more calories had better physical function at 3- but not at 6-months when assessed using the get up and go test, but not the 6-minute walk test.
Effective Interventions on Health Effects of Chinese Rural Elderly under Heat Exposure
Authors: Huang Yujia , Zhang Ting , Lou Jianing , Wang Peng , Huang Lei 
Published: February 2022

Abstract
Background: Due to climate change, the heatwave has become a more serious public health threat with aging as an aggravating factor in recent years. There is a pressing need to detect the most effective prevention and response measures. However, the specific health effects of interventions have not been characterized on an individual scale.Method: In this study, an intervention experiment was designed to explore the health effects of heat exposure at the individual level and assess the effects of different interventions based on a comprehensive health sensitivity index (CHSI) in Xinyi, China. Forty-one subjects were recruited randomly, and divided into one control group and three intervention groups. Interventions included education (Educate by lecturing, offering relative materials, and communication), subsidy support (offer subsidy to offset the cost of running air conditioning), and cooling-spray (install a piece of cooling-spray equipment in the yard).Results: Results showed that systolic blood pressure (SBP) and deep sleep duration (DSD) were significantly affected by short-term heat exposure, and the effects could be alleviated by three types of interventions. The estimated CHSI indicated that the effective days of the education group were longer than other groups, while the lower CHSI of the subsidy group showed lower sensitivity than the control group.Conclusions: Education and subsidy support can help reduce the effect of heat exposure efficiently. These findings provide feasible implementation strategies to optimize Heat-health action plans and evaluate the intervention performance.
Clinical Characteristics And Associated Factors Of Colonic Polyps In Acromegaly
Authors: Peng Guiliang , Li Xing , Zhou Yuanyuan , Bai Jianying , Hong Pian , Li Weixing , Zhang Yuling , Zhang Lei , Liao Qian , Liao Mingyu , Zhou Ling , Sun Zheng , Shen Rufei , Zheng Hongting , Long Min 
Published: February 2022

Abstract
Purpose: This study investigates the clinical characteristics and the associated factors of colonic polyps in patients with acromegaly.Methods: We retrospectively reviewed clinical characteristics and colonoscopy findings of 86 acromegalic patients who received treatment between August 2015 and July 2020 at Xinqiao Hospital. We analyzed colonoscopy findings and correlation with growth hormone (GH)-secreting pituitary adenoma (GHPA) volume and hormonal/metabolic levels.Results: Our analysis revealed that the prevalence of colonic polyps in acromegalic patients was 40.7% and increased significantly with advanced age, especially ≥50 years. Multiple polyps (62.8%) and colonic polyps in the left colon (54.2%) were detected more frequently. Compared to acromegalic patients without polyps, patients with polyps displayed higher IGF-1×ULN levels (P=0.03). IGF-1 levels and GHPA volumes in patients with polyps showed increasing trends, although there were no significant differences. Meanwhile, patients with polyps in the right colon showed higher GH levels and GH nadir (GHnadir) levels in the oral glucose tolerance test (OGTT) than those with polyps in the whole colon (P=0.023 and P=0.037, respectively). GH levels were higher in patients with polyps diameter ≤5mm than those with polyps diameter >5mm (P=0.031). In addition, the univariate and multivariate logistic regression analysis exhibited GHPA volumes (OR: 1.09, 95% CI: 1.01–1.20; P = 0.039) and IGF-1×ULN Q2 levels (OR: 6.51, 95% CI: 1.20–44.60; P=0.038) were independent factors for predicting the risk of colonic polyp occurrence in acromegalic patients. A nomogram was created to evaluate the risk of colonic polyps in acromegalic patients.Conclusion: The acromegalic patients are a high prevalence population of colonic polyps. Colonic polyps in acromegaly were multiple and frequently detected in the sigmoid colon and rectum. GHPA volumes and IGF-1×ULN levels may be predictors of colonic polyp occurrence.
Haploid Helps Phaeocystis Globosa Distribute to Deeper Dim Water, as Evidenced by Growth and Photosynthetic Physiological Responses to Different Irradiances
Authors: Zhuang Junlian , Lu Jiachang , Cao Kun-Fang , Li Jie 
Published: February 2022

Abstract
Phaeocystis globosa has a haplo-diplontic alternative life cycle and is an important causative species of harmful algal blooms. We hypothesize that the haploid cells of Phaeocystis globosa could better adapt to deep dim water than its diploid cells. Haploid and diploid solitary cells of P. globosa were cultured with eutrophic medium (f/2) under moderately low (15 µmol photons m− 2 s− 1), extremely low (5 µmol photons m− 2 s− 1) and normal (60 µmol photons m− 2 s− 1) irradiance conditions. The results showed that irradiances used in this study did not induce reactive oxygen species (ROS) damage in either haploid or diploid cells. Both haploid and diploid solitary cells grew faster at higher irradiance during the exponential growth phase. However, the haploid abundances under moderately and extremely low irradiances were higher than that under normal irradiance after the exponential growth phase, but diploid cells formed more colonies at higher irradiances. An increase in the photosynthetic pigments (PSC) ratio combined with a reduction in photoprotective pigments (PPC) ratio were found in both ploidies with decreasing irradiance, but the ratios of PSC and PPC and xanthophyll cycle pigments were significantly higher in haploid cells than in diploid cells. For haploids, the highest potential photochemistry efficiency of photosystems П was found under extremely low irradiance, but for diploids, it was observed under moderately low irradiance. The results suggest that both haploid and diploid solitary cells of P. globosa in eutrophic water can survive under low-light conditions, but haploid cells have an advantage in extremely low irradiance.
Deep ocean drivers better explain sperm whales Physeter macrocephalus than beaked whale habitat preferences in the Bay of Biscay
Authors: Virgili Auriane , Teillard Valentin , Dorémus Ghislain , Dunn Tim E. , Laran Sophie , Lewis Mark , Louzao Maite , Martínez-Cedeira José , Pettex Emeline , Ruiz Leire , Saavedra Camilo , Santos M.Begoña , Canneyt Olivier , Bonales José Antonio Vázquez , Ridoux Vincent 
Published: February 2022

Abstract
Species Distribution Models are commonly used with surface dynamic environmental variables as proxies for prey distribution to characterise marine top predator habitats. For oceanic species that spend lot of time at depth, surface variables might not be relevant to predict deep-dwelling prey distributions. We hypothesised that descriptors of deep-water layers would better predict the deep-diving cetacean distributions than surface variables. We combined static variables and dynamic variables integrated over different depth classes of the water column into Generalised Additive Models to predict the distribution of sperm whales Physeter macrocephalus and beaked whales Ziphiidae in the Bay of Biscay, eastern North Atlantic. We identified which variables best predicted their distribution. Although the highest densities of both taxa were predicted near the continental slope and canyons, the most important variables for beaked whales appeared to be static, surface and subsurface dynamic variables while for sperm whales, only surface and deep-water variables were selected. This could suggest differences in foraging strategies and in the prey targeted between the two cetacean taxa. A more systematic use of variables describing the deep-water layers would provide a better understanding of the oceanic species distribution and better assist in the planning of human activities in these habitats.
Primary Hepatic Synovial Sarcoma with Synchronous Colorectal Adenocarcinoma: A Case Report and Review of Literature
Authors: Vu Xuan Vinh , Nguyen Thanh Khiem , Nguyen Ham Hoi , Nguyen Ngoc Hung , Luong Tuan Hiep , Nguyen Cong Nguyen , Tran Van Chuong 
Published: February 2022

Abstract
Background: Primary hepatic synovial sarcoma is an extremely rare disease with a poor prognosis. The symptoms are nonspecific, and it is usually diagnosed at an advanced stage. Case presentation: A 63-year-old male patient was admitted with asymptomatic but elevated Alpha Fetoprotein (AFP) serum level in routine medical examination with a medical history of Hepatitis B with Tenofovir 300 mg per day. No sign of jaundice was observed. Her abdomen was flat and soft without tenderness or palpable mass. Dynamic abdominal computed tomography (CT) scan and magnetic resonance imaging (MRI) showed a hypervascular 15 x 26 mm mass within hepatic subsegment V. There was no distant spread location. Colonoscopy revealed 3-cm sessile polyp in Sigmoid colon with pathological result was adenocarcinoma. Immunohistochemistry was performed to accurate diagnosis. The tumor cells were positive for CD99, EMA and a Ki67 value of 40% and were negative for Hepar-1, CD34, CD117, CK7, STAT6, DOG1, SMA and S100. The final diagnosis for this patient was a Malignant Sigmoid-colon polyp with a Primary hepatic synovial sarcoma. Laparoscopic Sigmoid-colon and rectal resection with injecting autologous blood to mark the tumor before surgery was performed for malignant polypectomy lesion and Hepatic Radiofrequency Ablation (RFA) was performed four days before surgery. With no postoperative complications as well as acute hepatic failure, the patient was discharged on the POD8 uneventfully.Conclusion: A primary hepatic synovial sarcoma is especially rare, with the present case being the fifth accounted in the English literature. Synovial sarcoma (SS) is a genetically soft tissue sarcoma with uncertain differentiation and originated from primitive mesenchymal cells. We here present a report of this case with had primary hepatic synovial sarcoma synchronously with a colorectal adenocarcinoma.