bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multicolor light‐sheet microscopy for a large field of view imaging: A comparative study between Bessel and Gaussian light‐sheets configurations
Authors: Luna‐Palacios Yryx Y. , Licea‐Rodriguez Jacob , Camacho‐Lopez M. Dolores , Teichert Ines , Riquelme Meritxell , Rocha‐Mendoza Israel 
Published: February 2022

AbstractLight‐sheet fluorescence microscopy (LSFM) is useful for developmental biology studies, which require a simultaneous visualization of dynamic microstructures over large fields of views (FOVs). A comparative study between multicolor Bessel and Gaussian‐based LSFM systems is presented. Discussing the chromatic implications to achieve colocalized and large FOVs when both optical arrays are implemented under the same excitation objective is the purpose of this work. The light‐sheets FOVs, optical sectioning, and resolution are experimentally characterized and discussed. The advantages of using Bessel beams and the main drawbacks of using Gaussian beams for multicolor imaging are highlighted. Multiple Bessel excitation minimizes the FOV's mismatch's effects due to the beams chromatic defocusing and alleviates the aside object blurring obtained with multiple Gaussian beams. It also offers a fair homogeneous axial resolution and optical sectioning over a larger effective FOV. Imaging over perithecia samples of the fungus Sordaria macrospora demonstrates such advantages. This work complements previous comparative studies that discuss only single wavelengths light‐sheets excitations.
Low levels of serum IL‐39 are associated with autoimmune thyroid disease
Authors: Weng Linjie , Huang Guoqing , Gong Luping , Xu Jialu , Mao Yushan , Li Yan , Li Mingcai 
Published: February 2022

AbstractBackgroundInterleukin (IL)‐39 is a novel member of IL‐12 cytokine family, but its role in autoimmune thyroid diseases (AITD) is unclear. The aim of the present study was to determine serum levels of IL‐39 in Hashimoto's thyroiditis (HT) and Graves' disease (GD) patients.MethodsA total of 48 patients with HT, 50 patients with GD, and 45 healthy controls (HCs) were recruited for this study. Levels of serum IL‐39 were determined by ELISA.ResultsCompared with HC group, levels of serum IL‐39 in patients with HT (p < 0.05) and GD (p < 0.01) were drastically reduced. Among patients with HT, serum IL‐39 levels had a positive correlation with white blood cell count (WBC) count and free triiodothyronine level. Among patients with GD, the levels of IL‐39 in serum were positively correlated with WBC count and C‐reactive protein levels.ConclusionsIL‐39 may be a new potential predictor for patients with HT and GD.
Quantification of the impact of factors affecting the technical performance of operating room personnel: Expert judgment approach
Authors: Jafari Nodoushan Reza , Taherzadeh Chenani Khalil , Jahangiri Mehdi , Madadizadeh Farzan , Fallah Hossein 
Published: February 2022

AbstractIncorrect assessment of the performance shaping factors (PSFs), especially in health care systems, may lead to irreversible consequences such as death. The current study aimed to adapt PSFs taxonomy given in standardized plant analysis risk human reliability analysis (SPAR‐H) technique to surgical context. PSFs taxonomy of the SPAR‐H technique was revised, and more contextspecific definitions to surgical context were suggested. Step‐wise weight assessment ratio analysis (SWARA) and eleven‐digit numerical scale were used for quantification of the weight and negative influence rate of the PSFs during surgical processes, respectively. Nine PSFs were proposed following the SPAR‐H taxonomy. The order of the importance of the PSFs was quietly conflicting in terms of weight and negative influence rate. From the perspective of experts, fatigue and threat stress were assessed as the most important PSFs in terms of both weight and negative influence rate. Current study has offered a domain‐based understanding of the PSFs, particularly in the surgical context. Results of this study could be used to evaluate human error behaviors during the performance of tasks in the operating room. Moreover, these results could be used for improving patient safety programs in health care systems.
Semantic Context Search in Creative Idea Generation
Authors: Johnson Dan R. , Hass Richard W. 
Published: February 2022

AbstractThe ability to search memory for diverse contextual usages of words is proposed to play a role in creative idea generation. However, it is not yet known whether searching for more diverse contextual usages of words enhances the novelty of ideas during idea generation. In three experiments, participants were given a noun as a creativity prompt (e.g., chamber) and generated words creatively linked to the prompt (e.g., secret, relic). We directly manipulated the semantic diversity of creativity prompts to induce differential levels of semantic context search. Semantic diversity refers to the diversity of contexts in which a word is used (e.g., chamber orchestra vs. judge’s chamber). Semantic context search involves searching semantic memory for different contextual usages of a prompt word. Supporting the facilitative role of semantic context search, participants given prompts high in semantic diversity generated responses higher in novelty, compared to participants given prompts low in semantic diversity (Experiments 1 and 3). Capitalizing on recent advances in distributional semantics, our novel experimental approach led to a critical new insight—semantic context search can enhance the novelty of ideas.
Diacerein‐induced interleukin‐1β deficiency reduces the inflammatory infiltrate and immunoexpression of matrix metalloproteinase‐8 in periodontitis in rat molars
Authors: Silva Renata Cristina Lima , Sasso‐Cerri Estela , Cerri Paulo Sérgio 
Published: February 2022

AbstractBackgroundPeriodontitis is an immunoinflammatory disease that involves the release of cytokines and enzymes, including interleukin‐1 (IL‐1) and matrix metalloproteinases (MMPs). Diacerein is an anti‐IL‐1 drug used for the treatment of osteoarthritis. The aim of the study was to evaluate whether diacerein suppresses the inflammatory reaction and reduces the collagen degradation in the gingival connective tissue in periodontitis.MethodsFifty‐four male rats were distributed into three groups (n = 18 animals/group): 1) periodontitis + diacerein group (PDG), 2) periodontitis + saline group (PSG) and control Group (CG; without treatment). Periodontitis was induced for 7 days in the upper right first molars; after 7 days, the animals of PDG received 100 mg/kg of diacerein while in PSG, the animals received saline solution for 7, 15, and 30 days. The animals were killed and fragments of maxilla containing the right molars were processed for paraffin embedding. In hematoxlin & eosin‐stained sections, the volume density of inflammatory cells (VvIC) and fibroblasts (VvFb) in the gingival mucosa, the distances between the junctional epithelium (JE) to the cemento‐enamel junction (CEJ) and the CEJ to the alveolar process crest (AP) were obtained. The number of IL‐1β‒ and MMP‐8‒immunolabeled cells, and collagen content in the gingival mucosa were computed. Data were subjected to two‐way analysis of variance and Tukey post‐test (P≤ 0.05).ResultsThe PDG and PSG rats showed a significant increase in the distances of JE‐CEJ and CEJ‐AP. In all periods, the VvIC, the number of IL‐1β‒ and MMP‐8‒immunolabeled cells was significantly lower in PDG than in PSG while the collagen content was significantly greater in PDG than PSG. At 30 days, significant differences in the IL‐1β immunoexpression, collagen content, and in the MMP‐8 immunostaining were not seen between PDG and CG groups.ConclusionsOur results show an inhibitory effect of diacerein on IL‐1β in the inflamed gingival mucosa of rat molars, decreasing the inflammatory infiltrate and immunoexpression of MMP‐8, and restoring the collagen content.
Detection of multiple endocrine‐disrupting chemicals in milk: Improved and safe high performance liquid chromatography tandem mass spectrometry method
Authors: Yu Yunhan , Kuang Mengya , Zheng Baohua , Wang Meiling , Liu Zhenqiang , Xu Huilan , Wang Jianwu 
Published: February 2022

AbstractHerein, an improved method for detecting the endocrine‐disrupting chemicals in milk is presented, which is based on high performance liquid chromatography tandem mass spectrometry, coupled with a quick, effective, and safe method. The linearity of the proposed method was in the range of 0.05–100 μg/L, and all correlation coefficients were ≥0.9973. At three concentration levels, the spiked recoveries ranged from 77.7 to 107.5%, relative standard deviations ranged from 0.2 to 14.6%, limits of quantitation ranged from 0.1 to 40 μg/kg, limits of detection ranged from 0.03 to 13.3 μg/kg. The proposed method for the identification and quantitation of 26 endocrine disruptors present in milk is not only easy, fast, and cost‐efficient but also provides a reference for the detection of various endocrine disruptors in milk and other dairy products.
Bi‐amino acid functionalized biomimetic honeycomb chitosan membrane as a multifunctional hydrophilic probe for specific capture of N‐linked glycopeptides in nasopharyngeal carcinoma's disease patient's serum
Authors: Fu Mengyao , Wang Baichun , Yi Linhua , Jin Xueting , Yan Yinghua , Ding Chuan‐Fan 
Published: February 2022

AbstractIn this work, a novel porous bifunctionalized composite material was synthesized via a simple method. Gold nanoparticles are uniformly dispersed on the surface of the biomimetic honeycomb chitosan membrane through the interaction between amino and Au, and then cysteine and glutathione are successfully grafted onto the surface of the Au by the Au‐S bond. The modification of cysteine and glutathione makes this bifunctionalized composite material have significant advantages of superhydrophilicity and small steric hindrance simultaneously. This material manifests excellent property in glycopeptides enrichment, with high selectivity (1:5000), low detection limit (0.1 fmol·μL–1), high recovery rate (99.4 ± 0.5%), and good repeatability. In addition, with the help of nano‐flow liquid chromatography tandem mass spectrometry, this composite achieved excellent performance in efficiently enriching glycopeptides in the serum of healthy people and nasopharyngeal carcinoma's disease patient. More excitingly, further gene ontology analysis of molecular function and biological process indicated that 41 original glycoproteins of the identified glycopeptides from serum of nasopharyngeal carcinoma's disease patient significantly partake in numerous cancer‐associated events, including protease binding, calcium ion binding, enzyme binding, extracellular matrix organization, cellular response to tumor necrosis factor, and inflammatory response.
Effectiveness of psychosocial interventions in mitigating adverse mental health outcomes among disaster‐exposed health care workers: A systematic review
Authors: Ottisova Livia , Gillard Julia A. , Wood Maximillian , Langford Sarah , John‐Baptiste Bastien Rayanne , Madinah Haris Aishah , Wild Jennifer , Bloomfield Michael A.P. , Robertson Mary 
Published: February 2022

AbstractHealth care workers worldwide are at an increased risk of a range of adverse mental health outcomes, including posttraumatic stress disorder (PTSD), following the unprecedented demand placed upon them during the COVID‐19 pandemic. Psychosocial interventions offered to mitigate these risks should be based on the best available evidence; however, limited information regarding the comparative effectiveness of interventions is available. We undertook a systematic review of psychosocial interventions delivered to health care workers before, during, and after disasters. Eight databases were searched, including the Cochrane Central Register of Controlled Trials, PubMed/MEDLINE, EMBASE, and PsycINFO. Our primary outcomes were changes in symptoms of PTSD, anxiety, depression, and sleep. We calculated effect sizes, where unreported, and reliable change indices to appraise intervention effectiveness. The study was registered with PROSPERO (CRD42020182774). In total, 12,198 papers were screened, 14 of which were included in the present review. Interventions based on evidence‐based protocols, including individual and group‐based cognitive behavioral therapy (CBT) for PTSD, anxiety, and depression were found to lead to reliable changes in PTSD and anxiety symptoms. Single‐session debriefing and psychological first aid workshops showed limited efficacy. There is limited evidence on psychosocial interventions for health care workers faced with disasters, with the strongest evidence base for CBT‐based approaches. Future research should include controlled evaluations of interventions and aim to target identified risk factors.
Psychometric properties of the Chinese version of the Centrality of Event Scale across multiple trauma‐exposed Taiwanese samples
Authors: Kung Yi‐Wen , Su Yi‐Jen , Chen Sue‐Huei 
Published: February 2022

AbstractThe widely used Centrality of Event Scale (CES) measures the extent that a traumatic event serves as a central component of self‐identity, a reference point, and a turning point in an individual's life story. The present study aimed to develop a Chinese version of the CES and assess its reliability, criterion validity, and factor structure. Data were collected from three samples of trauma‐exposed Taiwanese individuals (N = 939), including 420 earthquake survivors, 300 trauma‐exposed community adults, and 219 trauma‐exposed undergraduate students. We conducted an exploratory factor analysis and confirmatory factor analysis and compared the resulting models with a one‐factor model and the originally proposed model. The results indicated that a new three‐factor model, S‐Bχ2(167, N = 519) = 687.01, p < .001, CFI = .95, IFI = .95, NNFI = .94, RMSEA = .078, SRMR = .047, might better represent the construct compared to the one‐factor or originally proposed model. Furthermore, the Chinese CES demonstrated excellent internal consistency, Cronbach's αs = .89–.94; adequate 1‐month reliability, rs = .54–.64, and 6‐month temporal stability, rs = .52–.67; and good concurrent and predictive validity. The findings indicate that the Chinese version of the CES demonstrates good psychometric properties with a three‐factor structure, and it could be used to assess event centrality among nonclinical trauma‐exposed Taiwanese adults.
Neutrophils and their extracellular traps impair ablative fractional carbon dioxide laser‐induced dermal remolding in mice
Authors: Zheng Wenyue , Zhu Zhenlai , Shi Yanqiang , Wen Si , Ye Li , Man Maoqiang , Yang Bin , Liu Zhenfeng 
Published: February 2022

AbstractObjectivesAblative fractional CO2 laser (AFL) therapy is an effective intervention to induce dermal remodeling. AFL treatment of the skin triggers the recruitment of immune cells, with neutrophils dominating the early phase. However, the role of recruited neutrophils in AFL‐induced microinjuries and their subsequent dermal remodeling capacity remains elusive.Materials and MethodsA mouse model of AFL‐induced dermal remodeling was established. RNA sequencing was used to identify the prominent features of AFL‐treated tissues. Histological analysis, including H&E and Masson staining, ultrastructure observation by transmission microscopy, immunofluorescence, and quantitative real‐time polymerase chain reaction were used for dermal remodeling analysis. Moreover, AFL‐treated mice were intraperitoneally injected with anti‐mouse Ly6G antibodies to deplete neutrophils. Neutrophil extracellular traps (NETs) were explored using immunofluorescence, transmission microscopy, and in vitro coculture experiments.ResultsDermal remodeling, characterized by an increased number of CD31‐positve vessels and elevated messenger RNA (mRNA) expression of genes encoding transforming growth factor‐β (TGF‐β), collagen I, and collagen III, was observed at 15 days after AFL treatment. In the AFL‐induced inflammation phase, RNA sequencing identified neutrophil chemotaxis, and degranulation genes were significantly enriched. Histology and immunofluorescence staining of human and mouse tissues harvested at Day 1 after AFL treatment revealed significant neutrophil infiltration surrounding thermal‐induced microinjuries. Neutrophil depletion decreased the expression of stress‐related genes such as S100A8 and S100A9 in the early phase following AFL treatment. Importantly, neutrophil depletion enhanced dermal remodeling at Day 15, as reflected by enrichment of the extracellular matrix and collagen biosynthesis genes based on RNA sequencing. Moreover, increased collagen I, collagen III, and TGF‐β mRNA expression, increased cell proliferation, and vascularity were observed. Interestingly, NETs, which could be induced by AFL‐treated fibroblasts in vitro, were identified in both human and mouse tissues on Day 1 after AFL treatment.ConclusionsAFL‐treated human and mouse skin recruited a large number of neutrophils. The neutrophil surge impaired dermal remodeling in mice. The microenvironment and fibroblast functional modulation mediated by neutrophil degranulation and NET formation were determined to be the underlying mechanisms. Our results indicate that modification of infiltrated neutrophil activity might be a potential therapeutic target for AFL‐induced dermal remodeling.
Understanding the Biological Interactions of pH‐Swellable Nanoparticles
Authors: Kermaniyan Sarah S. , Chen Moore , Zhang Changhe , Smith Samuel A. , Johnston Angus P. R. , Such Chris , Such Georgina K. 
Published: February 2022

AbstractpH‐responsive nanoparticles have generated significant interest for use as drug delivery systems due to their potential for inducible release at low pH. The pH variation from the bloodstream (pH 7.4) to intracellular compartments of cells called endosomes/lysosomes (pH < 5.0) has been of particular interest. However, one of the limitations with nanoparticle delivery systems is the inability to migrate out of these compartments to the cytosol or other organelles, via a process termed endosomal escape. Previous studies have postulated that pH‐responsive nanoparticles can facilitate endosomal escape through a range of mechanisms including membrane interaction, pH‐induced swelling, and the proton‐sponge effect. In this study, a series of pH‐swellable nanoparticles (85–100 nm) are designed and their impact on biological interactions, particularly endosomal escape, are investigated. The particles exhibit tunable pH‐induced swelling (from 120% to 200%) and have good buffering capacity. The cellular association is studied using flow cytometry and endosomal escape is determined using a calcein leakage assay. Interestingly, no endosomal escape with all nanoparticle formulations is found, which suggests there are limitations with both the proton‐sponge effect and pH‐induced swelling mechanism as the primary methods for inducing endosomal escape.
Hyaluronic‐Acid‐Coated Chitosan Nanoparticles for Insulin Oral Delivery: Fabrication, Characterization, and Hypoglycemic Ability
Authors: Wu Haishan , Guo Ting , Nan Jian , Yang Liu , Liao Guangfu , Park Hyun Jin , Li Jinglei 
Published: February 2022

AbstractOral administration of insulin faces multiple biological challenges, such as varied digestive environments, mucin exclusion, and low epithelial cells’ absorption. In the present study, a hyaluronic acid (HA)‐coated chitosan (CS) nanoparticle (HCP) delivery system is fabricated for insulin oral delivery. It is hypothesized that the developed nanoparticles will protect insulin from digestive degradation, promote intestinal epithelial cell absorption, and exert strong in vivo hyperglycemic ability. Nanoparticles formulated by CS and sodium tripolyphosphate (TPP) are optimized to form the core nanoparticles (CNPs). HA is further applied to coat CNP (HCP) to improve stability, reduce enzymatic degradation, and promote absorption of insulin. HCP promotes insulin uptake by Caco‐2 cells, absorbs less mucin, and improves intestinal absorption. Moreover, an in vivo test demonstrates that oral administration of insulin‐loaded HCP exerts strong and continuous hyperthermia effect (with a pharmacological availability (PA) of 13.8%). In summary, HCP is a promising delivery platform for insulin oral administration in terms of protecting insulin during digestion, facilitating its absorption and ultimately promoting its oral bioavailability.
Effect of retrogression re‐aging treatment on the stress corrosion behavior of the 7075 Al–Zn–Mg–Cu alloy
Authors: Liu Jinqiu , Guo Fuqiang , Wu Dongting , Matsuda Kenji , Wang Tao , Zou Yong 
Published: February 2022

AbstractThis paper studies the influence of the regression re‐aging (RRA) process on the mechanical properties and corrosion resistance of 7075. In the RRA heat treatment, the material properties are markedly affected by the variation of parameters in the regression stage. When the regression temperature and the regression duration increase, the mechanical properties of the material decrease. There was no intergranular corrosion in the samples treated by RRA, while obvious intergranular corrosion occurred in the T6 sample. Compared with the T6 sample, the RRA‐190‐0.5 sample with optimized parameters has a higher density of the precipitated phase and narrower precipitate‐free zone, and the precipitated phase along the grain boundary is discontinuous, isolated, and short. Compared with the single‐stage peak aging treatment, the RRA treatment can considerably improve the corrosion resistance and stress corrosion resistance but slightly reduce the strength. The best heat treatment process condition is 120°C/24 h + 190°C/0.5 h + 120°C/24 h.
A Comparison Study of Fiber Diameter's Effect on Characteristic Features of Donepezil/Curcumin‐Loaded Polycaprolactone/Polylactic Acid Nanofibers
Authors: Aydin Saliha , Kabaoglu Ilke , Guler Ece , Topal Fadime , Hazar‐Yavuz Ayse Nur , Ekentok Ceyda , Tatar Esra , Gurbuz Fatmanur , Gunduz Oguzhan , Cam Muhammet Emin 
Published: February 2022

AbstractNanofibers (NFs) offer an alternative option for the treatment of Alzheimer's disease (AD) by addressing unmet clinical problems. In this study, anti‐AD drugs, donepezil (DO) and curcumin (CUR), are loaded in polylactic acid/polycaprolactone NFs. The effect of fiber diameter on drug release behavior is mainly observed, and the successful loading of DO and CUR to NFs is demonstrated. The tensile strength of DO/CUR‐loaded NFs (DNFs) with lower fiber diameter is found to be higher. The working temperature is increased by the decrease of glass transition temperature and increase of the melting temperature after loading drugs. Furthermore, the increase in the percentage of swelling and decrease in the degradation rate for NFs are observed due to the increase of fiber diameter. Encapsulation efficiency and burst release percentages for DNFs are augmented by the increase of fiber diameter. Nevertheless, DNFs exhibit a sustained drug release manner over 2 weeks. NFs do not demonstrate a toxic effect on L929 (mouse fibroblast) cells, and additionally, they promote cell proliferation. Considering all these results, it is proven that the fiber diameter affects all characteristic features of NFs, and DNFs lead to a new and promising drug delivery system for the treatment of AD.