bluuebunny/crossref_metadata_2025_split huggingface dataset:
ApInAPDB: a database of apoptosis-inducing anticancer peptides
Authors: Faraji Naser , Arab Seyed Shahriar , Doustmohammadi Alireza , Daly Norelle L. , Khosroushahi Ahmad Yari 
Published: December 2022

AbstractApInAPDB (Apoptosis-Inducing Anticancer Peptides Database) consists of 818 apoptosis-inducing anticancer peptides which are manually collected from research articles. The database provides scholars with peptide related information such as function, binding target and affinity, IC50 and etc. In addition, GRAVY (grand average of hydropathy), net charge at pH 7, hydrophobicity and other physicochemical properties are calculated and presented. Another category of information are structural information includes 3D modeling, secondary structure prediction and descriptors for QSAR (quantitative structure–activity relationship) modeling. In order to facilitate the browsing process, three types of user-friendly searching tools are provided: top categories browser, simple search and advanced search. Overall ApInAPDB as the first database presenting apoptosis-inducing anticancer peptides can be useful in the field of peptide design and especially cancer therapy. Researchers can freely access the database at http://bioinf.modares.ac.ir/software/ApInAPDB/.
The El Niño Southern Oscillation drives multidirectional inter-reef larval connectivity in the Great Barrier Reef
Authors: Gurdek-Bas Rodrigo , Benthuysen Jessica A. , Harrison Hugo B. , Zenger Kyall R. , van Herwerden Lynne 
Published: December 2022

AbstractThe El Niño Southern Oscillation (ENSO) is the strongest source of interannual global climate variability, and extreme ENSO events are projected to increase in frequency under climate change. Interannual variability in the Coral Sea circulation has been associated with ENSO, although uncertainty remains regarding ENSO's influence on hydrodynamics and larval dispersal in the adjacent Great Barrier Reef (GBR). We investigated larval connectivity during ENSO events from 2010 to 2017 throughout the GBR, based on biophysical modelling of a widespread predatory reef fish, Lutjanus carponotatus. Our results indicate a well-connected system over the study period with high interannual variability in inter-reef connectivity associated with ENSO. Larval connectivity patterns were highly correlated to variations in the Southern Oscillation Index (SOI). During El Niño conditions and periods of weak SOI, larval dispersal patterns were predominantly poleward in the central and southern regions, reversing to a predominant equatorward flow during very strong SOI and extreme La Niña conditions. These ENSO-linked connectivity patterns were associated with positive connectivity anomalies among reefs. Our findings identify ENSO as an important source of variation in larval dispersal and connectivity patterns in the GBR, which can influence the stability of population dynamics and patterns of biodiversity in the region.
SUMO monoclonal antibodies vary in sensitivity, specificity, and ability to detect types of SUMO conjugate
Authors: Garvin Alexander J. , Lanz Alexander J. , Morris Joanna R. 
Published: December 2022

AbstractMonoclonal antibodies (MAb) to members of the Small Ubiquitin-like modifier (SUMO) family are essential tools in the study of cellular SUMOylation. However, many anti-SUMO MAbs are poorly validated, and antibody matching to detection format is without an evidence base. Here we test the specificity and sensitivity of twenty-four anti-SUMO MAbs towards monomeric and polymeric SUMO1-4 in dot-blots, immunoblots, immunofluorescence and immunoprecipitation. We find substantial variability between SUMO MAbs for different conjugation states, for detecting increased SUMOylation in response to thirteen different stress agents, and as enrichment reagents for SUMOylated RanGAP1 or KAP1. All four anti-SUMO4 monoclonal antibodies tested cross-reacted wit SUMO2/3, and several SUMO2/3 monoclonal antibodies cross-reacted with SUMO4. These data characterize the specificity of twenty-four anti-SUMO antibodies across commonly used assays, creating an enabling resource for the SUMO research community.
Benzyl trichloroacetimidates as derivatizing agents for phosphonic acids related to nerve agents by EI-GC-MS during OPCW proficiency test scenarios
Authors: Subramanian Alagu , Rosales José A. , Leif Roald N. , Valdez Carlos A. 
Published: December 2022

AbstractThe use of benzyl trichloroacetimidates for the benzylation of phosphonic acid nerve agent markers under neutral, basic, and slightly acidic conditions is presented. The benzyl-derived phosphonic acids were detected and analyzed by Electron Ionization Gas Chromatography–Mass Spectrometry (EI-GC–MS). The phosphonic acids used in this work included ethyl-, cyclohexyl- and pinacolyl methylphosphonic acid, first pass hydrolysis products from the nerve agents ethyl N-2-diisopropylaminoethyl methylphosphonothiolate (VX), cyclosarin (GF) and soman (GD) respectively. Optimization of reaction parameters for the benzylation included reaction time and solvent, temperature and the effect of the absence or presence of catalytic acid. The optimized conditions for the derivatization of the phosphonic acids specifically for their benzylation, included neutral as well as catalytic acid (< 5 mol%) and benzyl 2,2,2-trichloroacetimidate in excess coupled to heating the mixture to 60 °C in acetonitrile for 4 h. While the neutral conditions for the method proved to be efficient for the preparation of the p-methoxybenzyl esters of the phosphonic acids, the acid-catalyzed process appeared to provide much lower yields of the products relative to its benzyl counterpart. The method’s efficiency was tested in the successful derivatization and identification of pinacolyl methylphosphonic acid (PMPA) as its benzyl ester when present at a concentration of ~ 5 μg/g in a soil matrix featured in the Organisation for the Prohibition of Chemical Weapons (OPCW) 44th proficiency test (PT). Additionally, the protocol was used in the detection and identification of PMPA when spiked at ~ 10 μg/mL concentration in a fatty acid-rich liquid matrix featured during the 38th OPCW-PT. The benzyl derivative of PMPA was partially corroborated with the instrument's internal NIST spectral library and the OPCW central analytical database (OCAD v.21_2019) but unambiguously identified through comparison with a synthesized authentic standard. The method’s MDL (LOD) values for the benzyl and the p-methoxybenzyl pinacolyl methylphosphonic acids were determined to be 35 and 63 ng/mL respectively, while the method’s Limit of Quantitation (LOQ) was determined to be 104 and 189 ng/mL respectively in the OPCW-PT soil matrix evaluated.
Ventricular and lumbar cerebrospinal fluid analysis in 77 HIV-negative patients with Cryptococcal meningitis who received a ventriculoperitoneal shunt
Authors: Dong Qing , Huang Zhenchao , Yu Peng , Song Enpeng , Chen Zhijie , Qin Feng 
Published: December 2022

AbstractLumbar cerebrospinal fluid (CSF) parameters are widely studied and have wide clinical applications, but ventricular CSF has rarely been studied since it is relatively difficult to obtain. To determine whether there are differences between ventricular and lumbar CSF parameters and whether the differences have clinical significance, we retrospectively reviewed 77 patients with Cryptococcal meningitis who received a ventriculoperitoneal shunt. We analyzed the following parameters: white blood cell count, total protein concentration, CSF/blood glucose ratio, chloride ion concentration, and Cryptococcal count. All parameters between lumbar and ventricular CSF were remarkably different (all p < 0.001). White blood cell count, total protein level and Cryptococcal count were lower in ventricular CSF than in lumbar CSF, while CSF/blood glucose ratio and chloride ion concentration were higher. Compared to patients without ventriculomegaly, patients with ventriculomegaly had a significantly higher total protein concentration in ventricular CSF (p = 0.047). Compared to patients without surgical complications, patients with complications had a significantly lower CSF/blood glucose ratio in ventricular CSF (p = 0.032). The lumbar CSF parameters had no significant differences between these groups. The changes in lumbar CSF indices over time after shunt placement were also analyzed. After shunt placement, total protein concentration was transiently increased, white blood cell count, CSF/blood glucose ratio and chloride ion concentration were continued at the preoperative level until two months after shunting surgery. These findings suggest that the composition of ventricular CSF differs from that of lumbar CSF, and different CSF parameters have disparate rostro-caudal gradients in patients with Cryptococcal meningitis. Furthermore, ventricular and lumbar CSF parameters may have different clinical implications. Transient deterioration of lumbar CSF parameters after ventriculoperitoneal shunt placement may not be due to disease progression, but to change in CSF flow rate by CSF shunts.
Mechanochemical synthesis of alumina-based catalysts enriched with vanadia and lanthana for selective catalytic reduction of nitrogen oxides
Authors: Weidner Ewelina , Dubadi Rabindra , Samojeden Bogdan , Piasecki Adam , Jesionowski Teofil , Jaroniec Mietek , Ciesielczyk Filip 
Published: December 2022

AbstractNovel alumina-based materials enriched with vanadia and lanthana were successfully synthesized via in situ modification using a mechanochemical method, and were applied in ammonia-induced selective catalytic reduction of nitrogen oxides (SCR process). The synthesis was optimized in terms of the ball milling time (3 or 5 h), vanadium content (0.5, 1 or 2 wt% in the final product), and lanthanum content (0.5 or 1 wt% in the final product). Vanadium (V) oxide was immobilized on an alumina support to provide catalytic activity, while lanthana was introduced to increase the affinity of nitrogen oxides and create more active adsorption sites. Mechanochemical synthesis successfully produced mesoporous materials with a large specific surface area of 279–337 m2/g and a wide electrokinetic potential range from 60 to (− 40) mV. Catalytic tests showed that the incorporation of vanadia resulted in a very large improvement in catalytic performance compared with pristine alumina, increasing its efficiency from 14 to 63% at 400 °C. The best SCR performance, a 75% nitrogen oxide conversion rate at a temperature of 450 °C, was obtained for alumina enriched with 2 and 0.5 wt% of vanadium and lanthanum, respectively, which may be considered as a promising result.
Effects of match contextual factors on internal and external load in elite Brazilian professional soccer players through the season
Authors: Aquino Rodrigo , Guimarães Rodrigo , Junior Geraldo Oliveira Carvalho , Clemente Filipe Manuel , García-Calvo Tomas , Pulido Juan José , Nobari Hadi , Praça Gibson Moreira 
Published: December 2022

AbstractThis study aimed to investigate the effects of contextual match factors (quality of opposition, match outcome, change of head coach or playing style) on internal and external load in elite Brazilian professional soccer players, considering the total and effective playing time. Twenty-two professional male outfield soccer players participated in this study (age 28.4 ± 4.9 years; height 1.78 ± 0.1 cm; body mass 72.9 ± 7.1 kg). The internal (rating of perceived exertion-based load [sRPE]) and external load (distance and accelerometry-based measures) were recorded during 38 matches, over the 2021 season of the Brazilian National 1st Division League using a global position system (10 Hz) integrated with an accelerometer (200 Hz). The main results were: (i) matches played against weak opponents presented greater values of sprinting distances compared to matches against intermediate and strong opponents; (ii) players covered greater high-intensity running distances when drawing than winning the matches; (iii) matches with assistant coaches presented higher mean speed relative to effective playing time (MSEPT) compared to coach 1 and coach 2 conditions. In addition, players covered greater MSEPT and high-acceleration in matches with coach 2 vs. coach 3; (iv) finally, small positive correlations were observed between positional attack sequences and MSTPT, total distance covered, and acceleration. Coaches and practitioners should consider these results when interpreting external load variables during elite Brazilian soccer matches.
Experimental investigation of rotating nodal line of MEMS-based nonlinear multi-mode resonators
Authors: Liu Chun-You , Li Sheng-Shian 
Published: December 2022

AbstractNonlinear phenomenon is presently attracting considerable attention in the field of microelectromechanical systems (MEMS). By adjusting a controllable tuning voltage, the nonlinearity of microdevices, especially on microactuators, can be precisely manipulated. To trap and separate small particles, generating a large and stable rotation force is critical in micromanipulations. Here, we report a simple and potential angular momentum cell comprising a piezoelectric MEMS-based nonlinear multi-mode resonator with integrated electrodes. A nonlinear rotating nodal line has been observed in specific frequency bands by applying a controllable low voltage of sub 5 V on a 4-port resonator made of lead zirconate titanate (PZT) thin films. The magnitude of the actuated voltage is Complementary-Metal-Oxide-Semiconductor (CMOS)-compatible and easy to integrate with the circuit. Furthermore, the real-time rotation motion of the MEMS-based nonlinear multi-mode resonator is also verified by a laser doppler vibrometer (LDV) at both chirp and single input frequencies, respectively. Therefore, this angular momentum cell shows great potential in the application of micromanipulation.
Efficient co-production of EPA and DHA by Schizochytrium sp. via regulation of the polyketide synthase pathway
Authors: Ma Wang , Liu Mengzhen , Zhang Zixu , Xu Yingshuang , Huang Pengwei , Guo Dongsheng , Sun Xiaoman , Huang He 
Published: December 2022

AbstractPresently, the supply of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) traditionally produced by marine fisheries will be insufficient to meet their market demand in food industry. Thus a sustainable alternative source is urgently required. Schizochytrium sp. is an ideal producer of DHA; however, its ability to co-produce DHA and EPA has not yet been proved. Herein, we first described a cobalamin-independent methionine synthase-like (MetE-like) complex, which contains independent acyltransferase and 3-ketoacyl synthase domains, independent of the traditional polyketide synthase (PKS) system. When the MetE-like complex was activated, the EPA content was increased from 1.26% to 7.63%, which is 6.06-folds higher than that in the inactivated condition. Through lipidomics, we find that EPA is more inclined to be stored as triglyceride. Finally, the EPA production was enhanced from 4.19 to 29.83 (mg/g cell dry weight) using mixed carbon sources, and the final yield reached 2.25 g/L EPA and 9.59 g/L DHA, which means that Schizochytrium sp. has great market potential for co-production of EPA and DHA.
Aging is associated with a systemic length-associated transcriptome imbalance
Authors: Stoeger Thomas , Grant Rogan A. , McQuattie-Pimentel Alexandra C. , Anekalla Kishore R. , Liu Sophia S. , Tejedor-Navarro Heliodoro , Singer Benjamin D. , Abdala-Valencia Hiam , Schwake Michael , Tetreault Marie-Pier , Perlman Harris , Balch William E. , Chandel Navdeep S. , Ridge Karen M. , Sznajder Jacob I. , Morimoto Richard I. , Misharin Alexander V. , Budinger G. R. Scott , Nunes Amaral Luis A. 
Published: December 2022

AbstractAging is among the most important risk factors for morbidity and mortality. To contribute toward a molecular understanding of aging, we analyzed age-resolved transcriptomic data from multiple studies. Here, we show that transcript length alone explains most transcriptional changes observed with aging in mice and humans. We present three lines of evidence supporting the biological importance of the uncovered transcriptome imbalance. First, in vertebrates the length association primarily displays a lower relative abundance of long transcripts in aging. Second, eight antiaging interventions of the Interventions Testing Program of the National Institute on Aging can counter this length association. Third, we find that in humans and mice the genes with the longest transcripts enrich for genes reported to extend lifespan, whereas those with the shortest transcripts enrich for genes reported to shorten lifespan. Our study opens fundamental questions on aging and the organization of transcriptomes.
Developing Social-ABI-lity: An Online Course to Support Safe Use of Social Media for Connection After Acquired Brain Injury
Authors: Brunner Melissa , Rietdijk Rachael , Avramovic Petra , Power Emma , Miao Melissa , Rushworth Nick , MacLean Liza , Brookes Anne-Maree , Togher Leanne 
Published: December 2022

Purpose:
People who have an acquired brain injury (ABI) experience challenges using social media. Inversely, rehabilitation clinicians report feeling inadequately prepared to support them in its use. We aimed to develop a collaboratively designed, evidence-based online training resource to support people with an ABI to learn about using social media. Key recommendations for course design have previously been identified through a mixed methods approach, including (a) qualitative exploration of the experiences of people who use social media after ABI, (b) a scoping review to identify key features and effective teaching approaches from existing social media skills training programs.

Method:
Further design recommendations were obtained in this mixed methods study through (c) collaborative design of course content and features with 23 people, including people with living experience of ABI and other key stakeholders, and (d) a pilot trial of the course prototype with four participants who had an ABI (two men, two women; aged 28–69 years).

Results:
Training needs to be interactive, including practical components addressing online safety and wellbeing, and to explain how to use social media platforms to connect with others. The first social-ABI-lity prototype incorporated these findings. Pilot data indicated that the prototype was beneficial, with participants demonstrating small increases in social media confidence and knowledge. Areas for further refinement were also identified.

Conclusion:
The social-ABI-lity self-directed online course is the first of its kind to support people with an ABI in using social media and will be a valuable resource for rehabilitation clinicians internationally. This resource may drive sustainable changes in participation by helping people with ABI to build their social media mastery and to participate in supportive online networks.
A hybrid approach for energy‐efficient routing in IoT using duty cycling and improved ant colony
Authors: Rana Bharti , Singh Yashwant , Singh Pradeep Kumar , Ghafoor Kayhan Zrar , Shrestha Sachin 
Published: December 2022

AbstractThe promising technology of IoT in the fourth industrial revolution connects everything to the Internet in the digital world of the current era. Though the gigantic connectivity among things through the blend of multifarious technologies offers potential opportunities, it also increases the overhead to optimise the energy consumption in IoT networks. Energy optimisation has become the major concern in the IoT realm because of the continuous sensing of the constrained sensor nodes and data transmission to longer distances. The state‐of‐the‐art studies do not take into account the management of duty‐cycling and the process of efficient route discovery hand in hand. Also, the prevalent studies focus on the management of the sensor node's duty cycling (DC) solely instead of adaptive DC scheduling of the communication unit of the sensor node that consumes more energy during transmission. Therefore, keeping in view the existing tribulations regarding energy consumption, the authors attempt to devise an energy‐efficient routing approach using On‐Demand Duty Cycling and Ant‐Colony optimisation (DC‐ACO) for IoT. The ACO‐based routing approach is applicable in IoT networks because the ants’ environment is conceptualised as a distributed set of interconnected graph nodes. The proposed approach poses the empirical notion to manage the energy consumption of the IoT network by considering the key performance indicators (KPI) like energy consumption, packet delivery rate, average residual energy, mobility factor, distance, throughput, and network lifespan to accomplish the tangible outputs. The proposed approach is modelled using Data flow Diagrams (DFDs) and algorithms supported by results. Experimental results show significant improvement in relative throughput, network lifetime, and energy efficiency by 45%, 78%, and 68%, respectively, after simulating for successive iterations.
Betreuung Schwerkranker und Sterbender in der stationären
Versorgung
Authors: George Wolfgang , Sitte Thomas , Weber Karsten 
Published: December 2022

ZusammenfassungDie Ausgangssituation des Sterbens wird entlang der bestehenden
Sterbeortverhältnisse beschrieben. Ein Sterbeortregister fehlt in
Deutschland. Deutlich wird, dass bis heute ca. 80% aller
Bundesbürger:innen in stationärem Umfeld versterben. Nach
Ansicht verschiedener Expert:innen hat die Covid-19-Pandemie zu einem Verlust
erreichter Versorgungsqualitäten geführt. Die weiterhin
hervorragende Bedeutung einer gültigen Patienten:innenverfügung
für die letzte Lebensphase wird erkennbar.In einem Projekt wurde die bestehende Situation, dass 20–25% der
sterbenden Pflegeheimbewohner:innen nochmals in ein Krankenhaus verlegt werden,
zum Ausgangspunkt gewählt, um geeignete Lösungen zu entwickeln.
Auch innerhalb der Krankenhäuser kommt es zu Verlegungen Sterbender,
obwohl diese sehr häufig weder pflegerisch-medizinisch noch
psycho-sozial indiziert sind. Diese beiden Verlegungsprozesse betreffen
jährlich ca. 150 000 Patienten:innen bzw. Bewohner:innen. 13
ursächliche, sich zum Teil wechselseitig beeinflussende Faktoren, wurden
entlang von Vorarbeiten empirisch und faktorenanalytisch ermittelt. Unter
Begleitung des Marburger Instituts der Arbeitsgemeinschaft der
Wissenschaftlichen Medizinischen Fachgesellschaften (AWMF) wurden zwei
strukturiert-konsensbasierte Leitlinien entwickelt und in einer
Pflegeeinrichtung und einem Krankenhaus implementiert. Ergänzend zu
diesen Entscheidungshilfen wurden Leitfäden und Managementwerkzeuge
entwickelt, welche die Einführung unterstützen.Als Ergebnis konnte gezeigt werden, dass es nicht nur zu einer Reduktion der
Verlegungen, sondern auch zu einer Erhöhung der
Mitarbeiter:innenzufriedenheit kam. Die Beteiligten sehen die
Möglichkeit, die Leitlinien bundesweit zu transferieren. Das
gewählte Projektmanagement, die Anpassungsmöglichkeit der
entwickelten Instrumente an regionale Bedingungen, der intersektorale,
kollaborative und kommunale Ansatz ermöglichen dies.