bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examining Physical and Cognitive Function in Chronic Low Back Pain Through the Use of a Multisystem Resilience Framework
Authors: Bartley Emily J , Makhoul Melissa , Palit Shreela , Robinson Michael E , Fillingim Roger B 
Published: October 2022

AbstractObjectivesChronic pain results in significant impairment in older adults, yet some individuals maintain adaptive functioning. Limited research has considered the role of positive resources in promoting resilience among older adults. Likewise, these factors have largely been examined independently. We aimed to identify resilience domains based on biopsychosocial factors and explore whether resilience phenotypes vary across sleep disturbance, fatigue, and cognitive function.MethodsSixty adults (ages ≥60 years) with chronic low back pain completed measures of psychological, health, and social functioning. On the basis of previously published analyses, principal-components analysis was conducted to create composite domains for these measures, followed by cluster analysis to identify phenotypes.ResultsFour profiles emerged: Cluster 1, with high levels of psychosocial and health-related functioning; Cluster 2, with high health-related functioning and low psychosocial functioning; Cluster 3, with high psychosocial functioning and poorer health; and Cluster 4, with low levels of functioning across all domains. Significant differences across cluster membership emerged for sleep disturbance (ηp2 = 0.29), fatigue (ηp2 = 0.29), and cognitive abilities (ηp2 = 0.47). Individuals with the highest levels of resilience demonstrated more optimal outcomes in sleep and fatigue (P values ≤0.001) than did individuals with a less resilient phenotype. Furthermore, the High-Resilience group (Cluster 1) and the High Psychosocial / Low Health group (Cluster 3) had lower cognitive impairment than did the High Health / Low Psychosocial group (Cluster 2) and the Low-Resilience group (Cluster 4) (P values ≤0.009).ConclusionsA higher array of protective resources could buffer against the negative sequelae associated with chronic low back pain. These exploratory findings support the multidimensional nature of resilience and suggest that targeting resilience from a multisystem perspective might help to optimize interventions for older adults with chronic pain.
PBDE-47 induces impairment of mitochondrial biogenesis and subsequent neurotoxicity through miR-128-3p/PGC-1α axis
Authors: Tian Zhiyuan , Li Jing , Song Li , Xie Li , Li Dongjie , Xia Tao , Wang Aiguo 
Published: October 2022

Abstract
The potential adverse effects of 2,2′,4,4′-tetrabromodiphenyl ether (PBDE-47) on neurons are extensively studied, and mitochondria are identified as critical targets. This study aimed to investigate whether PBDE-47 impairs mitochondrial biogenesis via the miR-128-3p/PGC-1α axis to trigger mitochondrial dysfunction-related neuronal damage. In vitro neuroendocrine pheochromocytoma (PC12) cells and in vivo Sprague Dawley rat model were adopted. In this study, biochemical methods were used to examine mitochondrial ATP content, cell viability, and expressions of key mitochondrial biogenesis regulators, including peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), nuclear respiratory factor 1 (NRF1), and mitochondrial transcription factor A (TFAM). Mimics and inhibitors of miR-128-3p were employed to explore its role in PBDE-47-induced neurotoxicity. Both in vivo and in vitro evidences suggested that PBDE-47 suppressed PGC-1α/NRF1/TFAM signaling pathways and mitochondrial DNA (mtDNA) encoding proteins synthesis. PBDE-47 also suppressed the relative mtDNA content, mRNA levels of mtDNA-encoded subunits, and mitochondrial ATP levels in vitro. Specifically, 2-(4-tert-butylphenyl) benzimidazole (ZLN005) alleviated PBDE-47-induced neuronal death through the improvement of mitochondrial function by activating PGC-1α/NRF1/TFAM signaling pathways. Mechanistically, PBDE-47 dramatically upregulated miR-128-3p expression. Furthermore, miR-128-3p inhibition enhanced PGC-1α/NRF1/TFAM signaling and abolished PBDE-47-induced impairment of mitochondrial biogenesis. In summary, this study provides in vitro evidence to reveal the role of mitochondrial biogenesis in PBDE-47-induced mitochondrial dysfunction and related neurotoxicity and suggests that miR-128-3p/PGC-1α axis may be a therapeutic target for PBDE-47 neurotoxicity.
Changeable shared bus lane scheme for intersections based on lane signals
Authors: Liu Jiahui , Chen Dawei , Chen Qun 
Published: October 2022

Abstract
To address situations in which the bus lanes at intersections are idle for long periods, this paper proposes a changeable shared bus lane scheme for intersections based on lane signals. This scheme set up two GPS monitoring points for buses in the bus lane of the intersection to monitor the bus approaching the intersection. The lane signals linked to the monitoring points provide car drivers with a signal of whether they can enter the bus lane. This paper establishes an optimization model to maximize the number of vehicles entering the bus lane and uses numerical example simulations to discuss in detail the optimal monitoring point locations in different situations. Without affecting the bus, we obtain the optimal monitoring point setting schemes for several common situations and compare the total delay for ordinary vehicles in the bus lane of the lane-based signal scheme and a baseline case (without the lane-based signal). The results show that this scheme can effectively reduce vehicle delays at intersections. Finally, we discuss the limitations of this scheme and directions for future research.
RISK OF INTRAOCULAR INFLAMMATION AFTER INJECTION OF ANTIVASCULAR ENDOTHELIAL GROWTH FACTOR AGENTS
Authors: Patil Nikhil S. , Dhoot Arjan S. , Popovic Marko M. , Kertes Peter J. , Muni Rajeev H. 
Published: October 2022

Purpose:
This meta-analysis investigates the incidence of intraocular inflammation (IOI) after intravitreal antivascular endothelial growth factor injections in neovascular age-related macular degeneration.

Methods:
A systematic search was performed on Ovid MEDLINE, Embase, and Cochrane Central from January 2005 to April 2021. Randomized controlled trials comparing IOI after intravitreal bevacizumab, ranibizumab, brolucizumab, or aflibercept in neovascular age-related macular degeneration were included. Primary outcomes were sight-threatening IOI, final best-corrected visual acuity, and change in best-corrected visual acuity from baseline. Secondary outcomes included the incidence of other IOI events. Meta-analysis was performed using a random-effects model.

Results:
Overall, 11,460 unique studies were screened, of which 14 randomized controlled trials and 6,759 eyes at baseline were included. There was no difference between agents for the risk of endophthalmitis and retinal vascular occlusion. Compared with aflibercept, brolucizumab had a higher incidence of generalized IOI (risk ratio = 6.24, 95% confidence interval = [1.40–27.90]) and vitreous haze/floaters (risk ratio = 1.64, 95% confidence interval = [1.00–2.67]). There were no significant differences between comparators for other secondary end points.

Conclusion:
There was no difference in the risk of severe sight-threatening IOI outcomes between intravitreal antivascular endothelial growth factor agents. There was a significantly higher risk of generalized IOI after brolucizumab relative to aflibercept. Our results alongside other recent safety findings suggest the need for further investigation in the risk–benefit profile of brolucizumab for the treatment of neovascular age-related macular degeneration.
REFRACTIVE ERROR CHANGE DURING TREATMENT OF DIABETIC MACULAR EDEMA
Authors: Salabati Mirataollah , Mahmoudzadeh Raziyeh , Starr Matthew R. , Zhang Qiang , Sharpe James , Hsu Jason , Ho Allen C. , Regillo Carl , Kuriyan Ajay E. 
Published: October 2022

Purpose:
To evaluate the impact of anti–vascular endothelial growth factor therapy on the refractive error in eyes with diabetic macular edema.

Methods:
Post hoc analysis of Diabetic Retinopathy Clinical Research protocol T. Spherical equivalent (SE) was calculated for study and fellow eyes at baseline, 1-year, and 2-year visits. The SE change of the eyes with edema was compared with those with resolved edema. The correlation between refractive error changes and central subfield thickness was evaluated.

Results:
Among 543 study eyes, SE changed from −0.17 (2.04) D at baseline visit to −0.16 (2.14) D at 2 years giving a hyperopic shift of 0.04 (0.82) D (P = 0.022). Among fellow eyes, mean (SD) SE changed from −0.19 (2.1) D at baseline to −0.11 (2.1) D at 2 years, giving a hyperopic shift of 0.12 (0.84) D (P = 0.001). No significant difference in SE shift was found between eyes with and without edema at 2 years in phakic (0.12D and 0.08 D, P = 0.87) and pseudophakic eyes (−0.24D and −0.08D, P = 0.30). The SE shift was not correlated with central subfield thickness change at the end of the second year (r = 0.02, P = 0.62).

Conclusion:
Diabetic macular edema patients have minimal changes in refractive error. The correction of refractive error may be considered during treatment, regardless of the presence of edema.
Plastic Surgery Diversity, Equity, and Inclusion Mentorship Program and Workshop: A Single Institution’s Experience
Authors: Reghunathan Meera , Crowley Jiwon S. , Segal Rachel , Aguilar Diaz Marco Alejandro , Gosman Amanda A. 
Published: October 2022

Summary:
Several minority groups and students without home plastic surgery programs remain underrepresented in plastic surgery. To address these barriers, our division created the Plastic Surgery Diversity, Equity, and Inclusion Mentorship Program and Workshop. Students meeting at least one of the following eligibility criteria were invited in fall of 2020: medical student in years 1 though 3 or a research year who is (1) underrepresented in medicine, including African American, Hispanic/Latinx, Native American, Pacific Islander; (2) lesbian, gay, bisexual, transgender, or queer (LGBTQ); (3) first-generation low-income; or (4) does not have a plastic surgery residency program associated with their medical school. Students participated in quarterly meetings with their resident mentor and the annual hands-on workshop. The workshop was held on March 20, 2020, with both virtual and in-person participants. It included various didactic lectures, a suture laboratory, a resident panel, a social lunch hour, and extremity and head cadaver dissections with fracture plating. Twenty-four students attended the workshop. The course was rated 9.4 of 10 by students. The top met goals were gaining didactic plastic surgery knowledge (100%), exposure to a plastic surgery residency program (100%), resources to help overcome minority-specific barriers (96%), and technical skills such as suturing (96%). At the conclusion of the workshop, students had a significant increase in confidence in various aspects of plastic surgery knowledge and technical skills. The Plastic Surgery Diversity, Equity, and Inclusion Mentorship Program and Workshop demonstrated preliminary success in providing support for students underrepresented in medicine and students without home programs.
Postmortem Fetal Temperature Estimation with Magnetic Resonance Imaging: Apparent Diffusion Coefficient Measurements in the Vitreous Body and Cerebrospinal Fluid
Authors: Tijssen Maud P.M. , Hofman Paul A.M. , Robben Simon G.F. 
Published: October 2022

Objectives:
Magnetic resonance imaging (MRI) is increasingly used in postmortem fetal imaging. Several factors influence the quality of MRI in this setting, such as small size, autolytic and maceration changes, and temperature. Knowing the fetal temperature at the time of scanning can improve the MRI interpretation. Temperature can be calculated using diffusion-weighted imaging with measurements of the apparent diffusion coefficient (ADC) in the cerebrospinal fluid (CSF). However, this is complicated by small ventricle size and hemorrhage and, therefore, may be unreliable in postmortem imaging. The current study evaluated the feasibility and reliability of using the ADC for temperature measurements of the vitreous body compared to that of CSF.

Materials and Methods:
Two lambs were scanned postmortem at five different time points over 28 hours. Furthermore, 10 stillborn fetuses were scanned once, at 4 to 62 hours after birth. The temperature was measured with a digital thermometer and calculated using the ADCs of the vitreous body (lambs and fetuses) and CSF (fetuses).

Results:
There was an excellent correlation between measured and calculated temperatures in vitreous bodies of lambs (r = 0.997, P < 0.001) and fetuses (r = 0.970, P < 0.001). The correlation between measured and calculated temperatures in the CSF was poor (r = 0.522, P = 0.122).

Conclusion:
The calculation of the temperature based on the ADC of the vitreous body is feasible and reliable for postmortem fetal imaging.
Mediterranean-DASH Intervention for Neurodegenerative Delay diet and risk of dementia: three prospective studies and meta-analysis of cohort studies
Authors: Chen Hui , Dhana Klodian , Huang Yuhui , Huang Liyan , Tao Yang , Liu Xiaoran , van Lent Debora Melo , Zheng Yan , Ascherio Alberto , Willett Walter , Yuan Changzheng 
Published: October 2022

AbstractObjectiveTo evaluate the association of Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet with risk of dementia in three prospective cohort studies, and to conduct a meta-analysis of cohort studies.Design & SettingThree prospective cohort studies – the Whitehall II study (WII, mean follow-up=13.2 years), the Health and Retirement Study (HRS, mean follow-up=4.3 years) and Framingham Heart Study Offspring cohort (FOS, mean follow-up=10.4 years), and meta-analysis of cohort studies.ParticipantsWe included 18163 middle-aged and older women and men (8360 in WII, 6758 in HRS, and 3045 in FOS) without baseline prevalent dementia for cohort analyses.Main outcome measuresIncident all-cause dementia, with cohort-specific definitions.ResultsThe mean (SD) of baseline MIND diet score (range: 0-15) was 8.3 (1.4) in WII, 7.1 (1.9) in HRS, and 8.1 (1.6) in FOS. Over 171,079 person-years, a total of 802 participants (222 in WII, 338 in HRS, and 242 in FOS) developed incident dementia. In the multivariable-adjusted model, every 3-point increment in MIND diet score (range: 0-15) was related to 20% decreased risk of dementia (pooled hazard ratio (HR) = 0.80; 95% confidence interval (CI): 0.70-0.91, P-trend=0.0014, P-heterogeneity=0.3039). In the WII, the corresponding HRs (95% CIs) was 1.04 (0.71-1.52) for remote MIND score in 1991-1993, and 0.76 (0.53-1.09) for recent score in 2002-2004. In the FOS, the HRs were 0.66 (0.50-0.86) for MIND score in 1991-1995 and 0.67 (0.51-0.88) for that in 1998-2001, respectively. In the meta-analysis of eleven cohorts with 224,140 participants, the highest category of MIND diet score was related to 18% lower risk of dementia (pooled relative risk: 0.82, 95% CI: 0.74-0.92, I2=74%, P-heterogeneity<0.01) compared with the lowest category.ConclusionsAdherence to the MIND diet was related to lower risk of incident dementia in middle-aged and older adults. Further studies are warranted to develop and refine the specific MIND diet for different populations.Systematic review registrationINPLASY202270127Summary boxWhat is already known on this topic-Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet has been proposed as a healthy dietary pattern for human brain.-The relation of MIND to incident dementia was relatively inconsistent and inconclusive in previous cohort studies.What this study adds-Results from the cohort analyses and the meta-analysis confirmed that adherence to the MIND diet is prospectively related to lower risk of incident dementia.-Results in current studies are heterogeneous across different populations, calling for further investigations into the culture-specific MIND diet.
Associations of Cumulative Paternal and Maternal Childhood Maltreament Exposure with Neonate Brain Anatomy
Authors: Tuulari Jetro J. , Kataja Eeva-Leena , Karlsson Linnea , Karlsson Hasse 
Published: October 2022

AbstractBackgroundChildhood maltreatment exposure (CME) can lead to adverse long-term consequences for the exposed individual. Emerging evidence suggests that the long-term effect of CME may be transmitted across generations, starting already during prenatal development.MethodsIn this study, we measured brain grey and white matter volumes from MR images in 62 healthy neonates at 2–5 weeks of gestation corrected age and obtained Trauma and Distress Scale (TADS) questionnaire data from both parents.ResultsWe found that paternal CME associated positively with neonate supratentorial grey matter volumes while the association for the maternal TADS scores was not statistically significant. Maternal pre-pregnancy BMI associated with supratentorial white matter volumes, but not with parental CME.ConclusionsWe are the first to report that paternal CME is linked with variation in newborn cortical volume. Our results imply an intergenerational transmission of paternal CME to offspring. Elucidating the later relevance of these associations and mechanisms involved remains an enticing avenue for future studies.
A Deep-learning based RNA-seq Germline Variant Caller
Authors: Cook Daniel E. , Venkat Aarti , Yelizarov Dennis , Pouliot Yannick , Chang Pi-Chuan , Carroll Andrew , De La Vega Francisco M. 
Published: October 2022

AbstractRNA sequencing (RNA-seq) can be applied to diverse tasks including quantifying gene expression, discovering quantitative trait loci, and identifying gene fusion events. Although RNA-seq can detect germline variants, the complexities of variable transcript abundance, target capture, and amplification introduce challenging sources of error. Here, we extend DeepVariant, a deep-learning based variant caller, to learn and account for the unique challenges presented by RNA-seq data. Our DeepVariant RNA-seq model produces highly accurate variant calls from RNA-sequencing data, and outperforms existing approaches such as Platypus and GATK. We examine factors that influence accuracy, how our model addresses RNA editing events, and how additional thresholding can be used to facilitate our models’ use in a production pipeline.
Environmental gradients reveal stress hubs predating plant terrestrialization
Authors: Dadras Armin , Fürst-Jansen Janine M. R. , Darienko Tatyana , Krone Denis , Scholz Patricia , Rieseberg Tim P. , Irisarri Iker , Steinkamp Rasmus , Hansen Maike , Buschmann Henrik , Valerius Oliver , Braus Gerhard H. , Hoecker Ute , Mutwil Marek , Ischebeck Till , de Vries Sophie , Lorenz Maike , de Vries Jan 
Published: October 2022

ABSTRACTPlant terrestrialization brought forth the land plants (embryophytes). Embryophytes account for most of the biomass on land and evolved from streptophyte algae in a singular event. Recent advances have unraveled the first full genomes of the closest algal relatives of land plants; among the first such species wasMesotaenium endlicherianum. Here, we used fine-combed RNAseq in tandem with photophysiological assessment onMesotaeniumexposed to a continuous range of temperature and light cues. Our data establish a grid of 42 different conditions, resulting in 128 transcriptomes and ~1.5 Tbp (~9.9 billion reads) of data to study combinatory effects of stress response using clustering along gradients. We describe major hubs in genetic networks underpinning stress response and acclimation in the molecular physiology ofMesotaenium. Our data suggest that lipid droplet formation, plastid and cell wall-derived signals denominate molecular programs since more than 600 million years of streptophyte evolution—before plants made their first steps on land.
Protease inhibitor ASP enhances freezing tolerance by inhibiting protein degradation in Kumquat
Authors: Yang Hua , Qiao Ke-wei , Teng Jin-jing , Chen Jia-bei , Zhong Ying-li , Rao Li-qun , Li Huang , Xiong Xing-yao 
Published: October 2022

AbstractCold acclimation is a complex biological process leading to the development of freezing tolerance in plants. In this study, we demonstrated that cold-induced expression of protease inhibitor FmASP in a citrus relative species kumquat (Fortunella margarita(Lour.) Swingle) contributes to its freezing tolerance by regulating protein degradation. First, we found that only cold-acclimated kumquat plants, although with extensive leaf cellular damage during freezing, are able to resume their normal growth upon stress relief. To dissect the impact of cold acclimation on this extraordinary freezing tolerance, we performed protein abundance assay and quantitative proteomics analysis of kumquat leaves subjected to cold acclimation (4 °C), freezing treatment (−10 °C) and post-stress recovery (25 °C). FmASP and a few non-specific proteases were identified as differentially expressed proteins induced by cold acclimation and associated with stable protein abundance throughout the course of freezing treatment. FmASP was further characterized as a robust inhibitor that inhibits the degradation capacity of multiple proteases. In addition, heterogeneous expression ofFmASPin Arabidopsis confirmed its positive function in freezing tolerance. Finally, we proposed a working model of FmASP and illustrated how this extracellular-localized protease inhibitor protects proteins from degradation and consequently maintains essential cellular function for freezing stress recovery. These findings revealed the important role of protease inhibition on freezing response and provide insights on how this role may help develop new strategies to enhance plant freezing tolerance.One-sentence summaryA protease inhibitor ofFortunella margaritaenhances protein stability and freezing tolerance by regulating non-specific protease degradation
Fatigue presentation, severity, and related outcomes 12 weeks post-COVID-19 hospitalization
Authors: Magel Tianna , Meagher Emily , Boulter Travis , Albert Arianne , Tsai Melody , Muñoz Carola , Carlsten Chris , Johnston James , Wong Alyson , Shah Aditi , Ryerson Chris , Mckay Rhonda Jane , Nacul Luis 
Published: October 2022

AbstractBackgroundIncreasing evidence on long-term health outcomes following SARS CoV-2 infection shows post-viral symptoms can persist for months. The aim of the present study was to examine the prevalence and outcome predictors of post-viral fatigue and related symptoms 3 and 6 months following infection.MethodsA prospective cohort of patients hospitalized with COVID-19 (n=88) were recruited from a Post-COVID-19 Respiratory Clinic (PCRC) in Vancouver, Canada to examine predictors of long-term fatigue and substantial fatigue. Multivariable logistic and linear regression analysis were used to examine the relationship between patient predictors (medical history at the time of hospitalization and follow-up patient-reported outcome measures) and the presentation of fatigue and substantial fatigue at 3 and 6 months follow-up.ResultsThe number of patients exhibiting fatigue and substantial fatigue was 58 (67%) and 14 (16%) at 3 months and 47 (60%) and 6 (7%) at 6 months post-infection, respectively. Adjusted analysis revealed the number of pre-existing comorbidities to be associated with fatigue (OR 2.21; 95% CI 1.09-4.49; 0.028) and substantial fatigue (OR 1.73; 95% CI 1.06-2.95; 0.033) at 3 months follow-up. Except for shortness of breath, self-care, and time, all follow-up variables were found to be associated with fatigue and substantial fatigue at 3 months follow-up.ConclusionFatigue and substantial fatigue are common, and decrease from 3 to 6 months; however, a significant number of patients continue to exhibit long-term fatigue at 6 months follow-up. Further research is needed to clarify the causality of viral infections and co-factors in the development and severity of fatigue as a symptom and in meeting post-viral fatigue syndrome or ME/CFS diagnostic criteria.