bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low‐Intensity Pulsed Ultrasound Delays the Onset of Osteoporosis and Dyslipidemia in Mice With Premature Ovarian Insufficiency
Authors: Deng Juan , Li Junfen , Han Xinbin , Zhou Yiqing , He Sicheng , Xu Jie , Qin Juan , Song Guolin , Xiao Xinfang , Wu Liu , Wang Yan 
Published: January 2025

ObjectivesThe pathogenesis of premature ovarian insufficiency (POI) not only affects the ovarian structure and function but also gives rise to complications such as osteoporosis and dyslipidemia. Although low‐intensity pulsed ultrasound (LIPUS) has been proven effective in treating POI, its impact on the associated complications remains unexplored. Therefore, this study aims to investigate the effects of LIPUS irradiation on osteoporosis and dyslipidemia in a mouse model of POI.MethodsThe post‐treatment complications following LIPUS cessation were monitored in POI rats at 30 and 120 days in this trial conducted by us. The secretion of follicle‐stimulating hormone (FSH) was assessed using enzyme‐linked immunosorbent assay (ELISA) at different time points in POI rats. Pathological changes in the liver and femur were observed through HE staining. Blood lipid and bone metabolism indexes were measured via blood biochemical testing. Osteoporosis was evaluated using MicroCT and Masson staining techniques. The expression level of inflammatory factors in bone tissue was detected by Western blot (WB) test.ResultsThe serum FSH content showed a significant decrease in the LIPUS group on day 0, day 15, and day 30 (P < .05). On the 30th day following LIPUS treatment, TC, TG, and LDL‐C decreased in the LIPUS group while HDL‐C increased with no statistically significant differences (P > .05); the indexes of femur parameters (BS/TV, BV/TV, Tb.Th, BMD, and TMD) were increased (P > .05). On the 120th day after LIPUS treatment, TC content demonstrated a significant decrease (P < .05), and TG and LDL‐C displayed a downward trend (P > .05) while HDL‐C content increased (P < .05); the femur parameters were significantly reduced (P < .05). The LIPUS group exhibited an increased presence of new fibrillar fibers. Levels of IL‐6 and IL‐1β significantly decreased with LIPUS treatment, whereas osteocalcin (OCN) expression notably increased (P < .05).ConclusionsThe application of LIPUS demonstrates potential in mitigating complications associated with POI in rats through the reduction of FSH secretion levels and inhibition of tissue inflammation, thereby presenting a promising avenue for women with POI to explore more efficacious treatment alternatives.
Influence of Lubrication Viscosity on Dynamic Characteristics of Full‐Ceramic Bearings
Authors: Wang Zhan , Liu Zhenpeng , Wang Zinan , Zhou Peng , Xing Shiyu 
Published: January 2025

ABSTRACTFull‐ceramic bearings possess numerous exceptional attributes, such as enhanced rigidity and superior resistance to wear. Nevertheless, full‐ceramic bearings consistently encounter elevated temperatures for extended periods of high‐speed operation, which easily affect the processing performance of the equipment. Lubrication viscosity has a significant effect on bearing heat generation, so it is meaningful to approach the effect of lubrication viscosity with respect to the dynamics of full‐ceramic bearings. Full‐ceramic angular contact ball bearings are treated as research objects to analyse their optimal working condition in this article. A coupled fluid–solid simulation model is constructed for analysis of the fluid and solid in the bearing cavity. First, at the conditions of different lubricant viscosity, the oil volume distribution, temperature field distribution in the bearing cavity is analysed. Then, the vibration characteristics of the inner ring is examined by constructing a dynamic model of the inner ring. Meanwhile, temperature and vibration variation of full‐ceramic bearings are verified through experiments under different rotational speeds. The results show that the lubricant volume distribution inside the bearing cavity is nonuniformly distributed, which the lubricant is mainly located in the outer ring groove position. Moreover, elevating the lubricant viscosity within a certain range promotes the enhancement of bearing lubrication properties. The maximum error of the bearing temperature between the simulation results and the experiment is 7.592%. Ultimately, the simulation analysis is validated through experiments, and it provides a theoretical foundation for selecting optimal parameters for the oil–air lubrication of full‐ceramic bearing.
RNA N4‐acetylcytidine modification and its role in health and diseases
Authors: Wang Qiang , Yuan Yixiao , Zhou Qiang , Jia YingDong , Liu Jing , Xiao GuangJun , Li Chunhong , Jiang Xiulin 
Published: January 2025

AbstractN4‐acetylcytidine (ac4C) modification is a crucial RNA modification widely present in eukaryotic RNA. Previous studies have demonstrated that ac4C plays a pivotal role in viral infections. Despite numerous studies highlighting the strong correlation between ac4C modification and cancer progression, its detailed roles and molecular mechanisms in normal physiological processes and cancer progression remain incompletely understood. This review first outlines the key regulatory enzyme mediating ac4C modification, N‐acetyltransferase 10 (NAT10), including its critical roles in regulating RNA stability, transcriptional efficiency, and translational fidelity. Additionally, it systematically summarizes the essential functions and mechanisms of ac4C modification in normal biological processes, including stem cell fate determination, spermatogenesis and oogenesis, embryonic development, cellular senescence, and bone remodeling. Furthermore, this review delves into the central roles and molecular mechanisms of ac4C modification in regulating malignant proliferation, cell cycle arrest, EMT, drug resistance, cell death, cancer metabolism, and tumor immunotherapy. It also emphasizes the potential of NAT10 as a prognostic biomarker and its therapeutic potential as a target for disease treatment. In summary, this review clarifies the multifaceted roles of ac4C modification in both health and disease and explores NAT10‐targeted therapies with the aim of advancing cancer research and improving patient outcomes.
Reconstruction of Abdominal Defects After Open Abdomen Treatment Using Propeller Flaps of the Superior and Deep Inferior Epigastric Artery System: Report of Two Cases
Authors: Lam Martin C. , Henkel Andreas , Greber Lisa , von Websky Martin W. , Kalff Jörg C. , Walgenbach Klaus J. 
Published: January 2025

ABSTRACTOpen abdomen treatment (OAT) is associated with significant morbidity and mortality. In cases where primary or delayed fascial closure cannot be achieved, vacuum‐assisted wound closure and mesh‐mediated fascial traction are indicated, which often result in a planned ventral hernia. If secondary skin closure is not feasible, common treatment of granulated abdominal defects involves split‐thickness skin‐grafting or healing by secondary intention leading to significant scarring and sometimes mutilating defects. Late enteroatmospheric fistulae may develop as a result of instable scar tissue or insufficient soft tissue coverage. Perforator propeller flaps have been described for reconstruction of soft tissue defects of the abdomen; however, not for OAT‐induced abdominal defects. We report two complex cases of OAT‐induced abdominal wall defects of 20 × 8 and 22 × 10 cm, which were reconstructed with a propeller flap based on the superior epigastric artery perforator in the first case and the deep inferior epigastric artery perforator in the second case. The flaps were rotated into each of the abdominal defects following the propeller flap concept with primary closure of the donor sites and successful reconstruction of both defects. At 1‐year follow‐up, both patients developed asymptomatic incisional ventral hernias. Secondary‐stage abdominal wall reconstruction was not considered due to satisfaction with the reconstructive result and feared complications. Pedicled perforator flaps designed based on either the superior or deep inferior epigastric artery system are useful reconstructive options for midline abdominal defects without necessity for pedicle lengthening, microsurgical anastomosis, or another donor site beyond the abdomen. In conclusion, soft tissue coverage of OAT‐induced abdominal defects in critically ill patients can be achieved with the presented propeller flaps avoiding poor results of skin grafting or secondary intention healing.
Prediction of real‐time cine‐MR images during MRI‐guided radiotherapy of liver cancer using a GAN–ConvLSTM network
Authors: Jin Guodong , Liu Yuxiang , Wei Ran , Yang Bining , Pang Bo , Chen Xinyuan , Quan Hong , Dai Jianrong , Men Kuo 
Published: January 2025

AbstractBackgroundRespiratory motion during radiotherapy (RT) may reduce the therapeutic effect and increase the dose received by organs at risk. This can be addressed by real‐time tracking, where respiration motion prediction is currently required to compensate for system latency in RT systems. Notably, for the prediction of future images in image‐guided adaptive RT systems, the use of deep learning has been considered.PurposeThis study proposed a modified generative adversarial network (GAN) for predicting cine‐MR images in real time.MethodsSagittal cine magnetic resonance (cine‐MR) images of 15 patients with liver cancer who received RT were collected. The image series length of each patient was 300, and each series was divided into training, validation, and test sets. The datasets were further divided using a sliding window size of 10 and a stride of 1. A pix2pix GAN with the generator replaced by convolutional long short‐term memory (ConvLSTM) was proposed herein. A five‐frame cine‐MR image series was inputted into the network, which predicted the next five frames. The proposed network was compared with three advanced networks: ConvLSTM, Eidetic 3D LSTM (E3D–LSTM), and SwinLSTM. Personalized models were trained for each patient. The peak signal‐to‐noise ratio (PSNR), structural similarity index measure (SSIM), visual information fidelity (VIF), Pearson correlation coefficient (Pearson corr), and respiratory motion accuracy of the predicted images were used to evaluate the methods.ResultsThe proposed network demonstrated optimal performance in the four networks across various indicators. The proposed method provided better SSIM values than ConvLSTM at time steps 1, 2, 3, and 4, and outperformed E3DLSTM at all time steps. In terms of the VIF, the proposed method outperformed E3D–LSTM at all time steps and SwinLSTM at time steps 2, 3, 4, and 5. The proposed method was not significantly different from other methods in terms of Pearson correlation values except that it outperformed E3DLSTM at time step 1. In terms of the Pearson corr, the proposed method consistently achieves better values, especially in the high‐frequency components. Low average landmark tracking errors were provided by the proposed method at time steps 4 and 5 (2.42 ± 0.91 and 2.44 ± 0.96 mm, respectively).ConclusionsThe GAN–ConvLSTM network can generate high‐acutance real‐time cine‐MR images and predict respiratory motion with better accuracy.
Informing Implementation of a National Integrated Clinical Pathway for Low Back Pain in Ireland: A Pre‐Implementation Qualitative Study With General Practitioners
Authors: Murphy Cathriona , French Helen , McCarthy Geraldine , Albers Bianca , Cunningham Caitriona 
Published: January 2025

ABSTRACTIntroductionIreland's Health Service Executive is developing a new national integrated low back pain (LBP) pathway spanning primary and secondary care to improve LBP healthcare. Clinical pathways are frequently employed to optimise clinical outcomes and resource use but are challenging to implement. Context‐specific implementation planning, leveraging implementation science and its conceptual frameworks, should inform successful implementation.AimTo explore General Practitioner (GP) perceptions of the new LBP pathway, using their on‐the‐ground knowledge to assess barriers and facilitators to implementation with the goal of developing tailored implementation strategies.MethodsQualitative data will be collected through semi‐structured one‐to‐one interviews with a purposive sample of GPs. Data analysis will be guided by Framework Analysis with initial open coding followed by mapping to the domains and constructs of the Consolidated Framework for Implementation Science and Proctor's implementation outcomes of acceptability, appropriateness and feasibility. Potential implementation strategies will be devised from the identified determinants of implementation.ConclusionSystem‐wide changes and improvements in healthcare are difficult to accomplish. The rationale and design of a pre‐implementation study with key stakeholders is outlined with the view of informing optimal implementation of Ireland's new national integrated LBP pathway in primary care settings.
THE Key Elements of the Exercise Prescription Process in Musculoskeletal Rehabilitation in a One‐On‐One Setting. A Delphi Study of Australian Physiotherapy Specialists
Authors: Smith Samantha S. , Snodgrass Suzanne J. , Osmotherly Peter G. 
Published: January 2025

ABSTRACTBackgroundClinical decision making to develop an exercise program and then prescribe that program should be based on the best evidence available. However, little evidence exists to guide the development of an exercise program, determining optimal dosage parameters or for effective prescription techniques to achieve best patient outcomes possible.ObjectiveTo gain consensus from expert clinical physiotherapists in the field of musculoskeletal rehabilitation on key recommendations for developing and prescribing effective exercise programs in musculoskeletal rehabilitation in a one‐on‐one setting.MethodsDelphi study method involving three rounds of questionnaires with subsequent rounds developed based upon results of the previous round, aiming to achieve consensus in the final round. The consensus threshold was set at 80% agreement. Participants were physiotherapists considered experts in the field of musculoskeletal rehabilitation as acknowledged by the Australian College of Physiotherapists, being Clinical Fellows of the College in musculoskeletal or sports and exercise physiotherapy.ResultsThirteen experts completed the three rounds of questionnaires. Most experts reported ‘always’ prescribing exercises to their patients. The expert clinicians agreed that a clinician should blend evidence‐based protocols, clinical experience, and empirical knowledge to develop the exercise program, and then tailor the program further based on individual patient factors. Most experts agreed that it was essential to collaborate with the patient when designing the exercise program.ConclusionFuture research is needed to determine the effectiveness and necessity of each element of the exercise program in musculoskeletal rehabilitation in a one‐on‐one setting to achieve best patient outcomes possible.
Biotin Mitigates Alcohol Withdrawal‐Induced Anxiety and Depression by Regulating Serotonin Metabolism, BDNF, Inflammation, and Oxidative Stress in Rats
Authors: Hossaini Dawood , Alipour Adam Khan , Sajjadi Meysam , Ansari Mustafa , Haidary Murtaza 
Published: January 2025

ABSTRACTIntroductionSubstance use disorders, particularly alcohol use disorders, represent a significant public health problem, with adolescents particularly vulnerable to their adverse effects. This study examined the possible anxiolytic and antidepressant effects of biotin, a crucial vitamin for brain function, in attenuating the behavioral and neurobiological changes associated with alcohol withdrawal in adolescent rats.Materials and MethodsSixty male Sprague–Dawley rats were exposed to a 20% ethanol solution for 21 days, followed by a 21‐day drug‐free period to assess long‐term behavioral and physiological changes. Behavioral assessments included the Open Field Test, Elevated Plus Maze, and Forced Swimming Test, administered post‐withdrawal to evaluate anxiety and depression behaviors. Additionally, biochemical analyses were performed to measure serotonin levels, monoamine oxidase‐A (MAO‐A) activity, and BDNF concentrations.ResultsThe results indicate that ethanol withdrawal significantly induced anxiety‐ and depression‐like behavior in the rats. However, treatment with biotin, particularly at higher doses, effectively attenuated these withdrawal‐related behavioral changes. Mechanistically, biotin administration was found to regulate serotonin levels, monoamine oxidase activity, brain‐derived neurotrophic factor, and glial fibrillary acidic protein, and alleviate oxidative stress markers in cortical tissue.DiscussionThe results of this study suggest that biotin may have therapeutic potential for alleviating the negative effects of alcohol withdrawal, particularly those related to anxiety and depression. Further research is needed to elucidate the underlying mechanisms and examine the clinical effects of biotin supplementation for individuals undergoing alcohol withdrawal.
From crop to compost: Sonali Bag®—The biopolymer revolutionizing sustainable agriculture
Authors: Aftab Hrithita , Samadder Rajib , Rahman Md. Latifur , Ruhane Tania Akter , Khan Mubarak A. 
Published: January 2025

AbstractPlastic pollution, particularly in agriculture has reached alarming levels with over 400 million metric tons produced annually. This study evaluates the Sonali Bag® film (cellulose‐based biopolymer derived from jute) as a sustainable alternative to conventional plastic films used in agriculture. The film exhibits significant water absorption peaking at 1.45% within the first 10 min, due to its hydrophilic nature making it ideal for moisture retention in soil. Hydrolytic stability tests disclose that Sonali Bag® films degradation rates influenced by environmental conditions such as pH, temperature, and water salinity. At 100°C, the film fully dissolves while at lower temperatures it remains stable. Furthermore, porosity ranges from 83% to 96% depending on film thickness contributing to improved soil aeration and nutrient retention. Biodegradation tests show complete decomposition of the film within 100 days leaving no toxic residue. Thermal analysis (TGA) indicates the film remains stable up to 250°C, and DSC confirms a glass transition temperature (Tg) of 50°C. Fourier‐transform infrared spectroscopy and scanning electron microscopy analyses expose strong hydrogen bonding and a smooth porous surface respectively. These properties establish Sonali Bag® as an eco‐friendly, compostable material with potential to significantly reduce agricultural plastic waste while improving soil health and crop productivity.Highlights
Sonali Bag® a cellulosic sustainable alternative to agricultural plastic film.
Water absorption and biodegradation ensure no microplastic emission.
Hydrolytic stability influenced with salinity, temperature, and pH of water.
Porosity and bulk density enhance soil conditions and nutrient retention.
Structural analyses validate its suitability for various agricultural uses.
Sustainable Layer‐by‐Layer Films Using Chitosan Decorated With Anthocyanins and Carboxylated Graphene Oxide for Food Packaging Applications
Authors: Shen Jiaming , He Wendi , Zheng Yahui , Che Jianfei 
Published: January 2025

ABSTRACTThe abuse of traditional plastic food packaging has caused severe food safety and environmental pollution, urgently necessitating the development of green and sustainable biodegradable packaging materials. Chitosan (CS) belongs to an inexpensive and readily available biopolymer; however, the poor mechanical properties and weak resistance to UV light and water make it unsuitable for the food packaging industry. In this study, a layer‐by‐layer (LBL) assembly of CS decorated with anthocyanins as a polycationic electrolyte and carboxylated graphene oxide (GO‐COOH) as a polyanionic electrolyte is used to fabricate bio‐based films with the aim of improving comprehensive properties of CS‐based films. The results indicate that the LBL assembly extremely enhanced the UV‐blocking ability (the average transmittance in the UV‐B region drops to 1.68%), tensile strength (TS) (53.52 MPa), toughness (71.81%), thermal stability, water resistance, hydrophobicity (the water contact angle is up to 109.5°), and antioxidant activities of the films, attributed to hydrogen bonds among CS, anthocyanins, and GO‐COOH and electrostatic interactions between GO‐COOH and CS along with anthocyanins. In addition, the LBL films show exceptional advantages in the storage application for fresh‐cut apples. In general, the study demonstrates that a novel and sustainable CS‐based film can be fabricated by the LBL technique for food packaging.
Natural Products From Plants Targeting Leptin Resistance for the Future Development of Anti‐Obesity Agents
Authors: Liu Yu , Gong Fengying 
Published: January 2025

ABSTRACTObesity is a serious health threat, which has affected 16% of adults globally in 2022 and shows a trend toward youthfulness. Leptin, as a regulator of body weight, can suppress appetite and promote energy expenditure, making it potential in obesity treatment. Nevertheless, with the progress of relevant research, it is worth noting that monotherapy with leptin is not an effective strategy since most obese individuals are hyperleptinemic and resistant to leptin, where high levels of leptin fail to exert its weight‐loss effects. Therefore, the potential to unlock the weight‐loss properties of leptin using pharmacology to improve resistance has provided a new direction for this field. However, most synthetic medicines have retreated from the market due to their undesirable side effects, while natural products are increasingly sought after for drug development due to their minimal side effects. Indeed, natural products are ideal alternatives to oral synthetic agents since a growing body of research has demonstrated their desirable effects on improving leptin resistance through potential therapeutic targets like the JAK2/STAT3 signaling pathway, protein tyrosine phosphatase 1B, the exchange proteins directly activated by cAMP/Ras‐related protein 1 signaling pathway, endoplasmic reticulum stress, pro‐opiomelanocortin gene, and leptin levels. This review outlines natural products that can improve leptin resistance by inhibiting or activating these targets and evaluates their efficacy in experiments and human clinical trials, offering insights for the development of anti‐obesity agents. However, more high‐quality clinical research is necessary to validate these findings, as current clinical evidence is constrained by heterogeneity and small sample sizes.