bluuebunny/crossref_metadata_2025_split huggingface dataset:
Median lethal Dose and Acute Toxicity of Rosa canina L: In-Vivo Study
Authors: Zaman Mahmood Jasim  , Ghaith Ali Jasim  , Ibrahim Saleh Abbas  
Published: October 2022

Rosa canina belongs to rosacea family. Rosa. canina has a high concentration of phyto-constituents such as flavonoids, carotenoids, triterpene and vitamins as vitamin C, E, and A Rosa canina have an anti-inflammatory and antioxidant

effects. The antioxidant effect belongs to presence of large quantities of phytochemicals such as flavonoids and polyphenols. Rosa canina has been used for arthritis gout, osteoarthritis, urinary tract disorder, diabetes, inflammation and cancer. After grinding the leaves of Rosa canina, they are extracted by using ethanol solvent (cold extraction method), then the extract concentrated by rotary evaporator at 40 c° and leaving it to dry. Median lethal dose (LD50) has been examined on 84 mice (male and female) divided into seven groups, each one contains 12 (6 male and 6 female). The animals were monitored for signs and any behavior changes after administration of Rosa canina ethanol extract. Acute toxicity study was done on 20 rats (male and female for fourteen days. The weight of animals was taken at day 0, 7, and 14. At day fourteen, relative organ weight as well as histopathological examination for (heart, liver, spleen, kidney, lung, abdominal stomach, testes and ovaries) were taken. In addition to the serum biochemical tests for) blood glucose, urea, creatinine, ALT, AST and total bilirubin (were done at day fourteen. The result of this study, indicated that lethal dose 50 was 16.527 gram/kilogram. Acute toxicity study, revealed that there is no significant difference between relative organ weight of controlled and treated groups for all organs that were selected and mentioned above. In addition, there is no significant difference between serum biochemical tests for both controlled and treated groups. Finally, no changes have been found between controlled and treated groups regarding histopathology examination due to the p value was P ˃ 0.05.
Conclusion
According to the presented study, ethanol extract of Rosa canina showed wide range of safety depending on the result of lethal dose 50h (16.527 g/kg). Therefore, the extract considered nontoxic. No cytotoxic effect appeared by using ethanol extract of Rosa canina in acute toxicity study. This belongs to the results obtained, which include no significant difference between control and treated male and female rats in biochemistry tests and histopathological examination.
Atypical Features of Chikungunya Infection
Authors: Dutta Pradip Kumar , Dutta Arup , Rashid Md Mamunur , Dutta Sudipa 
Published: October 2022

Typical chikungunya virus (CHIKV) infection results in an acute febrile illness characterized by fever, severe joint pain and rash. Although chikungunya is generally not considered life threatening, atypical clinical manifestations resulting in significant morbidity have been documented, especially during epidemics. Our review is intended to elicit clinicians awareness of the atypical and severe forms of the disease in our region, as a way to potentially improve the outcomes of such life-threatening conditions.
JCMCTA 2017 ; 28 (1) : 3 - 7
Cerebrotendinous Xanthomatosis: Case Report on a Rare Genetic Disease
Authors: Chowdhury Goutam , Kayasthagir Pradip Kumar , Chowdhury Abhijit 
Published: October 2022

Cerebrotendinous Xanthomatosis (CTX) is a rare inborn progressive lipid storage disorder of autosomal recessive inheritance pattern.The multisystem disease causes decreased bile acid synthesis alongside cholesterol and cholestanol deposition in various tissue sites. This case report focuses on describing a middle-aged Bangladeshi male patient presented chiefly with neurological symptoms of CTX who received treatment with Chenodeoxycholic Acid (CDCA.
JCMCTA 2017 ; 28 (1) : 72 - 75
Higiene Sanitasi Pengolahaan dan Keberadaan Bakteri E. coli pada Es Teh di Warung Makan Kelurahan Mamboro Palu Utara
Authors: Politon Fellysca V. M. , Novarianti Novarianti 
Published: October 2022

Pendahuluan: Minuman es teh dapat terkontaminasi oleh beberapa jenis mikroba apabila pengolahannya tidak memenuhi standar kesehatan. Misalnya air tidak dimasak hingga mendidih. Mikroba yang terdapat pada minuman ini adalah jenis bakteri Escherichia coli. Tujuan: Tujuan penelitian untuk mengetahui higiene sanitasi pengolahan dengan keberadaan bakteri E. coli pada minuman Es Teh yang dijual Di Warung Makan Kelurahan Mamboro Palu Utara. Metode: Metode penelitian ini adalah deskriptif dengan pendekatan observasional pengolahan es teh dan pemeriksaan keberadaan bakteri E. coli pada es teh. Populasi dan sampel adalah 9 kantong plastik es teh masing-masing 1 kantong dari 9 warung makan di Kelurahan Mamboro Palu Utara. Hasil: Hasil penelitian, Higiene pengolahan makanan Tidak memenuhi syarat dengan nilai yang diperoleh 55% - <75%. sanitasi pengolahan dari hasil observasi mendapatkan 3 rumah makan dengan skor ≥ 75 % kategori memenuhi syarat dan 6 rumah makan nilai presentasi yang di dapat 55% - <75% kategori tidak memenuhi syarat). Pengujian 9 sampel ditemukan mengandung bakteri E. coli. Kesimpulan: Higiene pengolahan makanan tidak memenuhi syarat, Sanitasi pengolahan sebagian besar memenuhi syarat. Pada es teh yang dijual di Kelurahan Mamboro Palu utara 100% bernilai positif mengandung bakteri E. coli (Tidak Memenuhi Syarat).
Balloon pulmonary angioplasty can be an effective and safe therapeutic option in non-surgical elderly patients
Authors: Velázquez Martín Maite , Maneiro Melón Nicolás , Albarrán González-Trevilla Agustín , Sarnago Cebada Fernando , Huertas Nieto Sergio , Cruz-Utrilla Alejandro , Hinojosa Williams , López-Gude María Jesús , Alonso Charterina Sergio , Revilla Ostolaza Yolanda , Aguilar Colindres Ricardo José , Arribas Ynsaurriaga Fernando , Escribano Subias Pilar 
Published: October 2022

BackgroundAdvanced age, frailty, and age-related comorbidities are the major causes of pulmonary endarterectomy disqualification in patients with chronic thromboembolic pulmonary hypertension (CTEPH). Balloon pulmonary angioplasty (BPA) is an attractive and less invasive therapy for elderly patients. However, information about the safety, procedure tolerance, and effectiveness of BPA in elderly patients is limited.Objective and methodsWe aimed to analyze the safety, tolerance, and efficacy of BPA in CTEPH patients aged ≥70 years. This observational, descriptive, and retrospective series included consecutive patients aged ≥70 years, who underwent completed or interrupted BPA programs at a pulmonary hypertension reference center between May 2013 and May 2022.ResultsWe enrolled 155 patients in our institution's BPA program. Among these, 33 patients were aged ≥70 years (mean age, 76.4 years; women, 75.8%) and had finished or interrupted BPA programs. In this cohort, we performed 116 BPA procedures (average, 3.6 ± 1.8 sessions/patient). Among the 33 patients, 19 (57.6%) completed treatment for all lobes, while the BPA program was interrupted in the remaining 14 (42.4%). Among all 33 patients, BPA was associated with a significant reduction in mean pulmonary arterial pressure (39.2 ± 9.3 vs. 32.8 ± 8.8 mmHg; p &lt; 0.001) and pulmonary vascular resistance (6.7 ± 3.1 vs. 4.4 ± 2.0 WU; p &lt; 0.001), along with an improvement in the cardiac index (2.5 ± 0.6 vs. 2.8 ± 0.7 L/min/m2; p = 0.04) with significant reductions in the N-terminal prohormone of brain natriuretic peptide level (pre-BPA, 353 pg/mL [207–1,960 pg/mL] vs. post-BPA, 167 pg/mL [73–629 pg/mL]; p = 0.03). The patients' functional class improved, and pulmonary hypertension-targeting drug requirements were significantly reduced. The pulmonary injury appeared in 3.4% of the 116 procedures, of which 50% were of grade 2. No patient of ≥70 years had grade 5 pulmonary injury. One periprocedural mortality was recorded (3%), and the median follow-up period was 2.8 years. The survival rate of the entire cohort at 1 and 3 years was 90.5 and 82.8%, respectively.ConclusionBPA is an effective and safe approach in patients aged ≥70 years. It significantly improves patients' functional class, hemodynamic, and biomarkers, and reduces their pulmonary hypertension-targeting medical therapy requirements. These successes were achieved even though a significant percentage of patients did not complete the therapy. The rates of procedural complications and periprocedural mortality were low. Survival at 1 and 3 years was good in comparison to that of younger patients undergoing BPA.
Using machine learning to find genes associated with sudden death
Authors: Zhou Kena , Cai Congbo , He Yi , Chen Zhihua 
Published: October 2022

ObjectiveTo search for significant biomarkers associated with sudden death (SD).MethodsDifferential genes were screened by comparing the whole blood samples from 15 cases of accidental death (AD) and 88 cases of SD. The protein-protein interaction (PPI) network selects core genes that interact most frequently. Machine learning is applied to find characteristic genes related to SD. The CIBERSORT method was used to explore the immune-microenvironment changes.ResultsA total of 10 core genes (MYL1, TNNC2, TNNT3, TCAP, TNNC1, TPM2, MYL2, TNNI1, ACTA1, CKM) were obtained and they were mainly related to myocarditis, hypertrophic myocarditis and dilated cardiomyopathy (DCM). Characteristic genes of MYL2 and TNNT3 associated with SD were established by machine learning. There was no significant change in the immune-microenvironment before and after SD.ConclusionDetecting characteristic genes is helpful to identify patients at high risk of SD and speculate the cause of death.
A systematic review and Bayesian network meta-analysis comparing left bundle branch pacing, his bundle branch pacing, and right ventricular pacing for atrioventricular block
Authors: Zhang Yue , Jia Yuan , Liu Jia , Du Rongpin 
Published: October 2022

BackgroundAlthough right ventricular pacing (RVP) is recommended by most of the guidelines for atrioventricular block, it can cause electrical and mechanical desynchrony, impair left ventricular function, and increase the risk of atrial fibrillation. Recently, the His–Purkinje system pacing, including His bundle pacing (HBP) and left bundle branch pacing (LBBP), has emerged as a physiological pacing modality. However, few studies have compared their efficacy and safety in atrioventricular block (AVB).Methods and results:The PubMed, Web of Science, Cochrane Library, and ScienceDirect databases were searched for observational studies and randomized trials of patients with atrioventricular block requiring permanent pacing, from database inception until 10 January 2022. The primary outcomes were complications and heart failure hospitalization. The secondary outcomes included changes in left ventricular ejection fraction (LVEF) and left ventricular end-diastolic diameter (LVEDD), pacing parameters, procedure duration, and success rate. After extracting the data at baseline and the longest follow-up duration available, a pairwise meta-analysis and a Bayesian random-effects network meta-analysis were performed. Odds ratios (ORs) with 95% confidence intervals (CIs) or 95% credible intervals (CrIs) were calculated for dichotomous outcomes, whereas mean differences (MDs) with 95% CIs or 95% CrIs were calculated for continuous outcomes. Seven studies and 1,069 patients were included. Overall, 43.4% underwent LBBP, 33.5% HBP, and 23.1% RVP. Compared with RVP, LBBP and HBP were associated with a shorter paced QRS duration and a more preserved LVEF. HBP significantly increased the pacing threshold and reduced the R-wave amplitude. There was no difference in the risk of complications or the implant success rate. The pacing threshold remained stable during follow-up for the three pacing modalities. The pacing impedance was significantly reduced in HBP, while a numerical but non-significant pacing impedance decrease was observed in both LBBP and RVP. LBBP was associated with an increased R-wave amplitude during follow-up.ConclusionIn this systematic review and network meta-analysis, HBP and LBBP were superior to RVP in paced QRS duration and preservation of LVEF for patients with atrioventricular block. LBBP was associated with a lower pacing threshold and a greater R-wave amplitude than HBP. However, the stability of the pacing output of LBBP may be a concern. Further investigation of the long-term efficacy in left ventricular function and the risk of heart failure hospitalization is needed.Systematic review registration[https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=315046], identifier [CRD42022315046].
Left ventricular remodeling following aortic root and ascending aneurysm repair
Authors: Houben Ignas B. , Chu Angel K. Y. , Yang Bo , Kim Karen M. , Fukuhara Shinichi , van Herwaarden Joost A. , Moll Frans L. , Nordsletten David A. , Figueroa C. Alberto , Burris Nicholas S. , Patel Himanshu J. 
Published: October 2022

ObjectiveAdverse left ventricular remodeling due to a mismatch between stiffness of native aortic tissue and current polyester grafts may be under-recognized. This study was conducted to evaluate the impact of proximal aortic replacement on adverse remodeling of the left ventricle.Materials and methodsAll aortic root and ascending aortic aneurysm patients were identified (n = 2,001, 2006–2019). The study cohort consisted of a subset of patients (n = 98) with two or more electrocardiogram (ECG)-gated CT angiograms, but without concomitant aortic valve disease or bicuspid aortic valve, connective tissue disease, acute aortic syndrome or prior history of aortic repair or mitral valve surgery. LV myocardial mass was measured from CT data and indexed to body surface area (LVMI). The study cohort was divided into a surgery group (n = 47) and a control group; optimal medical therapy group (OMT, n = 51).ResultsThe mean age was 60 ± 11 years (80% male). Beta-blocker use was significantly more frequent in the surgery group (89 vs. 57%, p &lt; 0.001), whereas, all other antihypertensive drugs were more frequent in the OMT group. The average follow-up was 9.1 ± 4.0 months for the surgery group and 13.7 ± 6.3 months for the OMT group. Average LVMI at baseline was similar in both groups (p = 0.934). LVMI increased significantly in the surgery group compared to the OMT group (3.7 ± 4.1 vs. 0.6 ± 4.4 g/m2, p = 0.001). Surgery, baseline LVMI, age, and sex were found to be independent predictors of LVMI increased on multivariable analysis.ConclusionProximal aortic repair with stiff polyester grafts was associated with increased LV mass in the first-year post-operative and may promote long-term adverse cardiac remodeling. Further studies should be considered to evaluate the competing effects of aortic aneurysm related mortality against risks of long-term graft induced aortic stiffening and the potential implications on current size thresholds for intervention.
Immunotherapy response and microenvironment provide biomarkers of immunotherapy options for patients with lung adenocarcinoma
Authors: Zhan Xue , Feng Shihan , Zhou Xutao , Liao Wei , Zhao Bin , Yang Qian , Tan Qi , Shen Jian 
Published: October 2022

Background: Immunotherapy has been a promising approach option for lung cancer.Method: All the open-accessed data was obtained from the Cancer Genome Atlas (TCGA) database. All the analysis was conducted using the R software analysis.Results: Firstly, the genes differentially expressed in lung cancer immunotherapy responders and non-responders were identified. Then, the lung adenocarcinoma immunotherapy-related genes were determined by LASSO logistic regression and SVM-RFE, respectively. A total of 18 immunotherapy response-related genes were included in our investigation. Subsequently, we constructed the logistics score model. Patients with high logistics score had a better clinical effect on immunotherapy, with 63.2% of patients responding to immunotherapy, while only 12.1% of patients in the low logistics score group responded to immunotherapy. Moreover, we found that pathways related to immunotherapy were mainly enriched in metabolic pathways such as fatty acid metabolism, bile acid metabolism, oxidative phosphorylation, and carcinogenic pathways such as KRAS signaling. Logistics score was positively correlated with NK cells activated, Mast cells resting, Monocytes, Macrophages M2, dendritic cells resting, dendritic cells activated and eosinophils, while was negatively related to Tregs, macrophages M0, macrophages M1, and mast cells activated. In addition, ERVH48-1 was screened for single-cell exploration. The expression of ERVH48-1 increased in patients with distant metastasis, and ERVH48-1 was associated with pathways such as pancreas beta cells, spermatogenesis, G2M checkpoints and KRAS signaling. The result of quantitative real-time PCR showed that ERVH48-1 was upregulated in lung cancer cells.Conclusion: Our study developed an effective signature to predict the immunotherapy response of lung cancer patients.
Comprehensive analysis of cuproptosis-related genes in immune infiltration and diagnosis in ulcerative colitis
Authors: Huang Jinke , Zhang Jiaqi , Wang Fengyun , Zhang Beihua , Tang Xudong 
Published: October 2022

ObjectivesCuproptosis is a recently discovered form of programmed cell death; however, its role in ulcerative colitis (UC) remains a void.MethodsThree gene expression profiles were acquired from the GEO database. Subsequently, the single sample gene set enrichment analysis (ssGSEA) was performed to identify the immune infiltration characteristics of UC. Correlation analysis between cuproptosis and immune infiltration was further conducted, and the cuproptosis-related genes were applied to construct a UC diagnostic model. Subsequently, analysis results of microarray data were experimentally validated by DSS-induced colitis in mice. Finally, therapeutic agents for the cuproptosis-related genes were screened owing to the gaping field of therapeutic agents on cuproptosis.ResultsThree gene expression profiles with 343 samples (290 UC and 53 healthy samples) were included. Immune infiltration revealed that UC patients had a higher level of DCs, B cells, CD8+ T cells, iDCs, Macrophages, neutrophils, pDCs, T helper cells, Tfh, Th1 cells, Th2 cells, TIL and Treg than normal subjects. Moreover, almost all cuproptosis-related genes were significantly negatively associated with immune infiltration in UC patients. The risk prediction model based on cuproptosis-related genes showed an excellent discrimination for UC. Animal experiments revealed significant alterations in genes essential for cuproptosis between DSS-induced colitis mice and healthy controls, providing experimental validation for the analysis results of microarray data. Further analysis revealed that latamoxef, vitinoin, clomipramine, chlorzoxazone, glibenclamide, pyruvic acid, clindamycin, medrysone, caspan, and flavin adenine dinucleotide might be the target agents for cuproptosis-related genes.ConclusionsIn conclusion, cuproptosis was significantly associated with immune infiltration in UC, and the cuproptosis-related genes showed an excellent discrimination for UC.
Morphometric similarity networks discriminate patients with lumbar disc herniation from healthy controls and predict pain intensity
Authors: Yang Lili , Vigotsky Andrew D. , Wu Binbin , Shen Bangli , Yan Zhihan , Apkarian A. Vania , Huang Lejian 
Published: October 2022

We used a recently advanced technique, morphometric similarity (MS), in a large sample of lumbar disc herniation patients with chronic pain (LDH-CP) to examine morphometric features derived from multimodal MRI data. To do so, we evenly allocated 136 LDH-CPs to exploratory and validation groups with matched healthy controls (HC), randomly chosen from the pool of 157 HCs. We developed three MS-based models to discriminate LDH-CPs from HCs and to predict the pain intensity of LDH-CPs. In addition, we created analogous models using resting state functional connectivity (FC) to perform the above discrimination and prediction of pain, in addition to comparing the performance of FC- and MS-based models and investigating if an ensemble model, combining morphometric features and resting-state signals, could improve performance. We conclude that 1) MS-based models were able to discriminate LDH-CPs from HCs and the MS networks (MSN) model performed best; 2) MSN was able to predict the pain intensity of LDH-CPs; 3) FC networks constructed were able to discriminate LDH-CPs from HCs, but they could not predict pain intensity; and 4) the ensemble model neither improved discrimination nor pain prediction performance. Generally, MSN is sensitive enough to uncover brain morphology alterations associated with chronic pain and provides novel insights regarding the neuropathology of chronic pain.
The effects of supplementation of probiotics, prebiotics, or synbiotics on patients with non-alcoholic fatty liver disease: A meta-analysis of randomized controlled trials
Authors: Xing Wenmin , Gao Wenyan , Lv Xiaoling , Zhao Zhenlei , Mao Genxiang , Dong Xiaoyan , Zhang Zuyong 
Published: October 2022

BackgroundNon-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease. Research on the efficacy of probiotics, prebiotics, and synbiotics on NAFLD patients continues to be inconsistent. The purpose of this study is to evaluate the effectiveness of these microbial therapies on NAFLD.MethodsEligible randomized-controlled trials reporting the effect of probiotics, prebiotics, or synbiotics in NAFLD were searched in PubMed, Web of Science, Embase, Google scholar, and CNKI databases from 2020 to Jul 2022. The changes in the outcomes were analyzed using standard mean difference (SMD) and 95% confidence intervals (CIs) with a random- or fixed-effects model to examine the effect of microbial therapies. Subgroup analysis, influence and publication bias analysis were also performed. The quality of the eligible studies was evaluated using the Cochrane Risk of Bias Tool.ResultsEleven studies met the inclusion criteria involving 741 individuals. Microbial therapies could improve liver steatosis, total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL-c), alanine aminotransferase (ALT), alkaline phosphatase (ALP), glutamyl transpeptidase (GGT), and homeostasis model assessment-insulin resistance (HOMAI-R) (all P &lt; 0.05). But microbial therapies could not ameliorate body mass index (BMI), energy, carbohydrate, fat intake, fasting blood sugar, HbA1c, insulin, high-sensitivity C-reactive protein (hs-CRP), and hepatic fibrosis of patients with NAFLD.ConclusionProbiotics, prebiotics, and synbiotics supplementation can potentially improve liver enzymes, lipid profiles, and liver steatosis in patients with NAFLD.