bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced blood pressure in sickle cell disease is associated with decreased angiotensin converting enzyme (ACE) activity and is not modulated by ACE inhibition
Authors: Brito Pamela L. , dos Santos Alisson F. , Chweih Hanan , Favero Maria E. , Gotardo Erica M. F. , Silva Juliete A. F. , Leonardo Flavia C. , Franco-Penteado Carla F. , de Oliveira Mariana G. , Ferreira Wilson A. , Zaidan Bruna C. , Billis Athanase , Baldanzi Giorgio , Mashima Denise A. , Antunes Edson , Saad Sara T. Olalla , Costa Fernando F. , Conran Nicola 
Published: February 2022

BackgroundSickle cell disease (SCD) incurs vaso-occlusive episodes and organ damage, including nephropathy. Despite displaying characteristics of vascular dysfunction, SCD patients tend to present relatively lower systemic blood pressure (BP), via an unknown mechanism. We investigated associations between BP and renin-angiotensin-system (RAS) components in SCD and determined whether an inhibitor of angiotensin converting enzyme (ACE; often used to slow SCD glomerulopathy) further modulates BP and RAS components in a murine model of SCD.MethodsBP was compared in human subjects and mice with/without SCD. Plasma angiotensin II, ACE and renin were measured by immunoassay. BP was reevaluated after treating mice with enalapril (25 mg/kg, 5x/week) for 5 weeks; plasma and organs were stored for angiotensin II and ACE activity measurement, and quantitative real-time PCR.ResultsDiastolic BP and systolic BP were significantly lower in patients and mice with SCD, respectively, compared to controls. Reduced BP was associated with increased plasma renin and markers of kidney damage (mice) in SCD, as well as significantly decreased plasma ACE concentrations and ACE enzyme activity. As expected, enalapril administration lowered BP, plasma angiotensin II and organ ACE activity in control mice. In contrast, enalapril did not further reduce BP or organ ACE activity in SCD mice; however, plasma angiotensin II and renin levels were found to be significantly higher in enalapril-treated SCD mice than those of treated control mice.ConclusionRelative hypotension was confirmed in a murine model of SCD, in association with decreased ACE concentrations in both human and murine disease. Given that ACE inhibition has an accepted role in decreasing BP, further studies should investigate mechanisms by which ACE depletion, via both Ang II-dependent and alternative pathways, could contribute to reduce BP in SCD and understand how ACE inhibition confers Ang II-independent benefits on kidney function in SCD.
Epigenetic clock analysis and increased plasminogen activator inhibitor-1 in high-functioning autism spectrum disorder
Authors: Okazaki Satoshi , Kimura Ryo , Otsuka Ikuo , Funabiki Yasuko , Murai Toshiya , Hishimoto Akitoyo 
Published: February 2022

Background
Autism spectrum disorder (ASD) is characterized by impaired social communication and behavioral problems. An increased risk of premature mortality has been observed in individuals with ASD. Therefore, we hypothesized that biological aging is accelerated in individuals with ASD. Recently, several studies have established genome-wide DNA methylation (DNAm) profiles as ‘epigenetic clocks’ that can estimate biological aging. In addition, ASD has been associated with differential DNAm patterns.

Methods
We used two independent datasets from blood samples consisting of adult patients with high-functioning ASD and controls: the 1st cohort (38 ASD cases and 31 controls) and the 2nd cohort (6 ASD cases and 10 controls). We explored well-studied epigenetic clocks such as HorvathAge, HannumAge, SkinBloodAge, PhenoAge, GrimAge, and DNAm-based telomere length (DNAmTL). In addition, we investigated seven DNAm-based age-related plasma proteins, including plasminogen activator inhibitor-1 (PAI-1), and smoking status, which are the components of GrimAge.

Results
Compared to controls, individuals with ASD in the 1st cohort, but not in the 2nd cohort, exhibited a trend for increased GrimAge acceleration and a significant increase of PAI-1 levels. A meta-analysis showed significantly increased PAI-1 levels in individuals with ASD compared to controls.

Conclusion
Our findings suggest there is no epigenetic age acceleration in the blood of individuals with ASD. However, this study provides novel evidence regarding increased plasma PAI-1 levels in individuals with high-functioning ASD. These findings suggest PAI-1 may be a biomarker for high-functioning ASD, however, larger studies based on epigenetic clocks and PAI-1 will be necessary to confirm these findings.
Prevalence of biofilms in Candida spp. bloodstream infections: A meta-analysis
Authors: Atiencia-Carrera María Belén , Cabezas-Mera Fausto Sebastián , Tejera Eduardo , Machado António 
Published: February 2022

Context
Candida-related infections are nowadays a serious Public Health Problem emerging multidrug-resistant strains. Candida biofilm also leads bloodstream infections to invasive systemic infections.

Objective
The present meta-analysis aimed to analyze Candida biofilm rate, type, and antifungal resistance among hospitalized patients between 1995 and 2020.

Data sources
Web of Science, Scopus, PubMed, and Google Scholar databases were searched for English papers using the following medical subject heading terms (MESH): “invasive candidiasis”; “bloodstream infections”; “biofilm formation”; “biofilm-related infections”; “mortality”; and “prevalence”.

Study selection
The major inclusion criteria included reporting the rate of biofilm formation and the prevalence of biofilm-related to Candida species, including observational studies (more exactly, cohort, retrospective, and case-control studies). Furthermore, data regarding the mortality rate, the geographical location of the study set, and the use of anti-fungal agents in clinical isolates were also extracted from the studies.

Data extraction
Independent extraction of articles by 2 authors using predefined data fields, including study quality indicators.

Data synthesis
A total of 31 studies from publicly available databases met our inclusion criteria. The biofilm formation in the data set varied greatly from 16 to 100% in blood samples. Most of the studies belonged to Europe (17/31) and Asia (9/31). Forest plot showed a pooled rate of biofilm formation of 80.0% (CI: 67–90), with high heterogeneity (Q = 2567.45, I2 = 98.83, τ2 = 0.150) in random effects model (p < 0.001). The funnel plot and Egger’s linear regression test failed to find publication bias (p = 0.896). The mortality rate in Candida-related bloodstream infections was 37.9% of which 70.0% were from biofilm-associated infections. Furthermore, Candida isolates were also characterized in low, intermediate, or high biofilm formers through their level of biofilm mass (crystal violet staining or XTT assays) after a 24h growth. When comparing between countries, statistical differences were obtained (p = 0.0074), showing the lower and higher biofilm prevalence values in Italy and Spain, respectively. The prevalence of low, intermediate, and high biofilms were 36.2, 18.9, and 35.0% (p < 0.0001), respectively. C. tropicalis was the prevalent species in high biofilm formation (67.5%) showing statistically significant differences when compared to other Candida species, except for C. krusei and C. glabrata. Finally, the rates of antifungal resistance to fluconazole, voriconazole, and caspofungin related to biofilm were 70.5, 67.9 and 72.8% (p < 0.001), respectively.

Conclusions
Early detection of biofilms and a better characterization of Candida spp. bloodstream infections should be considered, which eventually will help preserve public health resources and ultimately diminish mortality among patients.
Rancang Bangun Sistem Informasi Pengukuran Bobot Daging Lembu Berbasis Android
Authors: M.Kom Sumarsono 
Published: February 2022

Pengukuran bobot daging hewan sapi perlu mempertimbangkan faktor kecermatan, ketepatan dan kemudahan. Dalam era teknologi yang terus berkembang khususnya smartphone diharapkan mamberikan kontribusi positif dalam melakukan perhitungan bobot daging hewan qurban. Setiap tahun panitia penyembelihan hewan qurban dituntut untuk memprediksi bobot sehingga akan diketahui lebih dini berat bersih daging seluruh hewan qurban. Menghitung bobot menggunakn teori kombinasi dengan dua komponen yaitu lingkar dada dan panjang badan. Hasil dari penelitian ini adalah terbentuknya sistem dan alat yang mampu memprediksi secara otomatis bobot daging hewan qurban, melakukan proses kalkulasi data hewan qurban yaitu mulai dari mendeteksi panjang lingkar dada hewan (cm), menghasilkan berat daging bersih (kg), hak shohibul sebanyak 1/3 daging hewan (kg), hak 1/7 shohibul jika jumlah shohibul sebanyak 7 orang per hewan(kg), hingga sisanya dibagikan kepada musthik (kg). seluruh proses otomasi kalkulasi daging hewan qurban ini menggunakan teknologi smartphone berbasis Android yang memiliki keunggulan dan visualisasi interface (antarmuka) perangkat sehingga lebih user friendly ( ramah bagi pengguna).
Experimental and simulation assessment to mitigate the emission of sulfide toxic gases and removing main impurities from Zn + Pb + Cu recovery plants
Authors: Saidi Majid , Kadkhodayan Hossein 
Published: February 2022

Abstract
An integrated novel approach employing the Taguchi method and Aspen Plus software has been applied to evaluate a new configuration for the industrial process of Zn + Pb + Cu recovery from sphalerite ore, in order to minimize the toxic gas emission. The optimum operating condition achieved by the Taguchi method has been used as initial data for the process simulation. The impact of operating parameters on the process performance is considered. The optimum condition for the conversion of sulfide toxic gases to H2SO4 have been found to be: acid concentration of 0.867 mol/L, reaction temperature of 120 °C, stirring speed of 400 rpm, leaching time of 120 min, sulfide ore particle size of 0.01 mm; solid-to-liquid ratio of 30 wt%, additives amount of 50 kg/ton and oxygen pressure of 200 psi. Under optimum condition, H2SO4 production from sulfide toxic gases is 99%, the removal percentage of Fe, Co, Mn, Ni and Cd impurities is 99% and the recovery percentage of Zn + Pd + Cu is more than 97%.
Existence and uniqueness of the weak solution for Keller-Segel model coupled with Boussinesq equations
Authors: Slimani Ali , Bouzettouta Lamine , Guesmia Amar 
Published: February 2022

Abstract
Keller-Segel chemotaxis model is described by a system of nonlinear partial differential equations: a convection diffusion equation for the cell density coupled with a reaction-diffusion equation for chemoattractant concentration. In this work, we study the phenomenon of Keller-Segel model coupled with Boussinesq equations. The main objective of this work is to study the global existence and uniqueness and boundedness of the weak solution for the problem, which is carried out by the Galerkin method.
On convergence of explicit finite volume scheme for one-dimensional three-component two-phase flow model in porous media
Authors: Mostefai Mohamed Lamine , Choucha Abdelbaki , Cherif Bahri 
Published: February 2022

Abstract
In this work, we develop and analyze an explicit finite volume scheme for a one-dimensional nonlinear, degenerate, convection–diffusion equation having application in petroleum reservoir. The main difficulty is that the solution typically lacks regularity due to the degenerate nonlinear diffusion term. We analyze a numerical scheme corresponding to explicit discretization of the diffusion term and a Godunov scheme for the advection term.

L

∞

{L}^{\infty }

stability under appropriate CFL conditions and

BV

{\rm{BV}}

estimates are obtained. It is shown that the scheme satisfies a discrete maximum principle. Then we prove convergence of the approximate solution to the weak solution of the problem, and we mount convergence results to a weak solution of the problem in

L

1

{L}^{1}

. Results of numerical experiments are presented to validate the theoretical analysis.
Experiments on liquid film thickness around Taylor finger
Authors: Kumar Amit , Thakur Amit K. , Gaurav Gajendra Kumar , Ravuru Narasimha Reddy 
Published: February 2022

Abstract
Taylor finger is characterized by a single elongated air bubble that grows upward during the drainage of liquid from closed top vertical tubes. The characteristic of the Taylor finger is similar to the Taylor bubble commonly observed in gas–liquid two-phase flow. During the upward growth of the Taylor finger, liquid from the tube drains as a thin film around it. The exact prediction of film thickness is important in several engineering designs and process calculations such as the design of contacting devices, two-phase flow through porous media, boiling in tubes, and monolith reactors. The present study proposes an experimental technique to estimate the thickness of the draining liquid film. Based on experiments an empirical model has been proposed for non-dimensional film thickness in the inviscid region. The proposed model agrees well with the experimental data and equation proposed in published literatures (Davies, R. M., and G. Taylor. 1950. “The Mechanics of Large Bubbles Rising Through Extended Liquids and Through Liquids in Tubes.” Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences 200, 375–90; Dukler, A. E., and J. Fabre. 1994. “Gas-Liquid Slug Flow.” Multiphase Science and Technology 8: 1–4; Fabre, J., and A. Liné. 1992. “Modeling of Two-Phase Slug Flow.” Annual Review of Fluid Mechanics 24: 21–46).
Numerical algorithm for nonlinear fractional equations with nonlocal boundary conditions based on a modified minimum residual method
Authors: Jiang Wei , Yue Zihan , Chen Zhong , Wu Fei 
Published: February 2022

Abstract
In this paper, we will solve a nonlinear time-fractional equation with nonlocal boundary conditions. First, we construct a set of suitable base according to the correlation theory of reproducing kernel space and the nonlocal boundary conditions. Then we introduce F-derivative and Newton iterative to linearize the nonlinear terms of the equation. The appropriate initial iteration value is constructed by boundary conditions. Finally, we use the ɛ-approximate solution theory to solve the system of linear equations and give the corresponding theoretical derivation. In conclusion, several numerical examples illustrate the feasibility and effectiveness of the method.
Application of mining algorithm in personalized Internet marketing strategy in massive data environment
Authors: Pan Qianqian , Yang Gang 
Published: February 2022

Abstract
Internet marketing requires a personalized marketing strategy. In this study, the application of data mining in personalized Internet marketing was studied. Based on the mining algorithm, a personalized marketing method was designed. Through the calculation of frequent closed item sets and support counts of positive and negative samples, the interval with a high success rate for marketing was obtained. With performance analysis, it was found that the success rate of the marketing method proposed in this study improved 8% compared with the traditional marketing method and had a better performance under the smaller interval number and smaller minimum success number. After applying the designed method in telecommunication enterprise A, it was found that after adopting the marketing method of this study, the marketing success rate of enterprise A increased from 2.72 to 6.31%, which indicated the effectiveness of the method. The research results of this study verify the role of data mining algorithms in Internet marketing, which is conducive to the further application of mining algorithms in personalized marketing and innovation of business modes.
Dental formula variations in wild and domestic Sus scrofa: is the first premolar agenesis an evolutionary trend?
Authors: Azorit Concepción , Oya Antonia , Hohl Leandro S. L. , Carrasco Rafael , Rocha-Barbosa Oscar , Casinos Adrià 
Published: February 2022

Abstract
We investigated the occurrence of congenital dental abnormalities in 94 wild boars Sus scrofa scrofa, and 20 Iberian pigs Sus scrofa domesticus from Doñana (DNP) and Sierra de Andújar Natural Park (ANP) southern Spain. The only dental variation found was agenesis of the lower first premolar, either deciduous (Dp1) or permanent (P1). We analyzed prevalence variations using: odds ratios, Fisher’s exact test and logistic-regression models. The lower first premolar has often been reported to be absent in modern domestic breeds of pigs, but it is usually reported as present in wild boar. However, we found a similar occurrence of agenesis in wild boar as in Iberian pigs. A common genotypic background between wild boar and Iberian pig populations of ANP sharing the same ecosystems may explain this result. When considering only wild boar, unexpected differences between populations were detected. The wild boar from ANP showed higher estimated probabilities of agenesis than those from DNP. Environmental conditions can also represent influencing factors for changes within subspecies. Our findings reinforce the idea that in suids the congenital absence of premolars probably has an evolutionary relevance, which could represent a generalized trend towards the reduction of the dental formula from the plesiomorphic placental dentition.
Puberty and sex in pediatric thyroid cancer: could expression of estrogen and progesterone receptors affect prognosis?
Authors: da Silva Breder Julia Ramalho Amalio , Alves Paulo Alonso Garcia , Araújo Mario Lucio , Pires Barbara , Valverde Priscila , Bulzico Daniel Alves , Accioly Fernanda Andrade , Corbo Rossana , Vaisman Mario , Vaisman Fernanda 
Published: February 2022

Objective
A sharp increase in pediatric thyroid cancer incidence is observed during adolescence, driven mainly by girls. Differences in disease presentation across sexual maturity stages raise the question of whether sex steroids have a role in the heterogeneity. The aims of this study were to analyze the influence of puberty and sex on clinical presentation and prognosis and to evaluate the correlation between the expression of sex hormone receptors.

Design and methods
Clinical records and immunohistochemical of specimens from 79 patients were analyzed. Puberty was analyzed by two criteria: end of puberty and beginning, in which the age of 10 was the cutoff.

Results
Postpubertal were more frequently classified as having low-risk disease and a lower frequency of persistent disease, especially when the completion of puberty was used as the criteria. Male sex was associated with a higher risk of persistent disease at the end of the observation period. Estrogen receptor α positivity was low in the entire sample, while progesterone receptor positivity was positive in 30% of the cases. Female hormone receptor expression was not associated with sex, American Thyroid Association risk score, persistent structural disease, or pubertal status.

Conclusion
Our study showed that the completion of puberty correlated best with the clinical behaviour of pediatric thyroid cancer. It was also shown that postpubertal patients have a less aggressive initial presentation and better outcomes. However, this observation could not be explained by the expression of estrogen and progesterone receptors in the primary tumors.
Makna Agama dan Budaya di dalam Foto Karya Rony Zakaria Berjudul Men, Mountains and the Sea
Authors: Sayyida Hana , Ridho Kholis 
Published: February 2022

Foto documenter bertema hubungan manusia, gunung dan laut karya Rony Zakaria berjudul Men, Mountains and the Sea menurut penulis penting dikaji. Karena di dalamnya merepresentasikan tata cara hidup yang diyakini, dijalani, dan dikembangkan sebagai pandangan hidup umumnya masyarakat Jawa. Akulturasi agama dan budaya, yang diantaranya dicerminkan dari upacara keagamaannya, merupakan bukti bahwa agama tidak hadir dalam ruang yang hampa budaya. Foto dokumentasi tersebuttidak saja bernilaihistoris, tetapi juga ke depan dapat menjadi pijakan habituasi cultural bagi masa depan generasi suku Jawa.Penelitian ini menggunakan paradigma konstruktivis dengan pendekatan kualitatif melalui model analisis semiotika Roland Barthes. Yakni memberi titik tekan pada makna denotasi, konotasi, dan mitos yang ditafsirkan melalui prespektif makna budaya yang dikembangkan dalam keilmuan antropologi, sosiologi dan sejarah.Hasil analisa buku foto ini didapatkan kesimpulan bahwa manusia Jawa tidak dapat meninggalkan kelestarian alam semesta sebagai bagian dari kehadiran Tuhan di dunia. Alam semesta itu dapat berupa gunung dan laut, yang juga adalah penghidupan yang melekat dalam keseharian mereka. Meskipun pergeseran zaman telah memasuki sistem keyakinan keagamaan mereka, namun agama local tetap bertahan dan terus hidup di masyarakatnya.
Mutation in mgrB is the major colistin resistance mechanism in Klebsiella pneumoniae clinical isolates in Tehran, Iran
Authors: Khoshbayan Amin , Shariati Aref , Razavi Shabnam , Baseri Zohre , Ghodousi Arash , Darban-Sarokhalil Davood 
Published: February 2022

Abstract
Colistin is considered as one of a last resort antimicrobial agent against multidrug-resistant Gram-negative bacteria including Escherichia coli and Klebsiella pneumoniae. However, the recent emergence of colistin resistance (ColR) worldwide that severely restricts therapeutic options is a serious threat to global public health. In this study we have investigated the molecular determinants in ColR K. pneumoniae isolates collected from clinical specimens.
A total of 98 E. coli and 195 K. pneumoniae clinical isolates were collected from two hospitals from August 2018 to December 2019 in Tehran, Iran. Colistin susceptibility and minimum inhibitory concentrations (MIC) were determined according to the Clinical and Laboratory Standards Institute by disk diffusion method, and microdilution method, respectively. For isolates with colistin MIC ≥4 μg mL−1, PCR was performed for the detection of mcr-1 to mcr-4 genes. Moreover, nucleotide sequences of mgrB, phoP, phoQ, pmrA, and pmrB genes were determined by sequencing. Finally, the transcriptional level of pmrK and pmrC genes was evaluated by quantitative reverse transcription PCR (RT-qPCR).
None of the E. coli isolates were resistant to colistin while 21 out 195 K. pneumoniae isolates were identified as resistant, 19 of which carried mutation in the mgrB gene. Three different mutations were observed in the pmrB gene in 3 K. pneumoniae isolates. None of the ColR isolates showed alternations in pmrA, phoP, and phoQ genes. Furthermore, none of the plasmid-encoding genes were detected. Transcriptional level of the pmrK gene increased in all ColR isolates meanwhile, pmrC overexpression was detected in 16 out 21 (76.19%) isolates. Eventually, all ColR isolates were susceptible to tigecycline. Our results demonstrated that the alternation of mgrB gene is the main mechanism related to colistin resistance among ColR K. pneumoniae isolates in this study.