bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of annexin V levels and cytokine status in patients with acute myocardial infarction
Authors: Naumov A. V. , Prokofieva T. V. , Polunina O. S. , Saroyants L. V. 
Published: October 2022

Aim. Study of annexin V levels and cytokine status in patients with acute myocardial infarction. Materials and methods. We examined 27 patients with Q-forming myocardial infarction who were admitted 12–24 hours after the development of the clinic, and 20 individuals in the control group. The lipid spectrum was determined using an automatic analyzer Humalizer-3000 (Human, Germany). Immunological studies were performed on a flow cytofluorimeter Navios (Beckman Coulter, USA). Lymphocyte subpopulations were evaluated using monoclonal antibodies produced by Immunotech (Coulter Corporation, USA). A set of ANNEXIN V-FITC/7 AAD (Beckman Coulter, USA) was used to isolate cells that had undergone apoptosis. Isolation of peripheral blood mononuclears was performed by centrifugation on a density gradient Ficol-hypac (Pharmacia Fine Chemicals, Sweden), interleukin-1β was determined using the Vector-Best test system (Vector-Best, Russia), and the tumor necrosis factor was determined using the RayBio Human TNF-alpha ELISA Kit (USA). Statistical data processing was performed using the program package Statistica 10.0. Results. In patients with acute myocardial infarction on the background of dyslipidemia (total cholesterol and LDL cholesterol increased, cholesterol-HDL reduced) showed a significant decrease in the total number of T-lymphocytes (СD 3+) (p < 0.05), T-suppressor (СD 8+) (p < 0.01), B-cells number (СD 19+) and the ratio of CD 4+/CD 8+ lymphocytes increased (p < 0.05). In patients with myocardial infarction there was a significant increase of apoptotic cells and рro-inflammatory cytokines involved in apoptosis (IL-1β and TNF-α) in the blood compared with the control. Conclusion. Increased spontaneous apoptosis of peripheral blood lymphocytes in patients with acute myocardial infarction indicates the systemic nature of apoptosis in acute coronary pathology, due to increased annexin-dependent apoptosis of T-lymphocytes as a result of immune disorders, which leads to activation of the cytokine system with an increased concentration of рro-inflammatory mediators (TNF-α, IL-1β). The identified dependencies require further study with the prospect of developing predictive algorithms for myocardial infarction outcomes based on indicators of annexin V and pro-inflammatory cytokines.
One-stage dental implantation without orthopedic loads – histological and radiographic control of survival
Authors: Studenikin R. V. , Filin A. A. , Mamedov A. A. , Niftaliev K. S. 
Published: October 2022

Purpose: histological and radiographic study and comparison of osseointegration and stability of implants without orthopedic load, installed in different ways (simultaneously in a fresh socket immediately after tooth extraction and in mature bone).Materials and methods: the criterion for inclusion in the study was the possibility of placing a test (immediately after tooth extraction) and a control implant (in mature bone) on opposite sites without the use of osteoplastic materials or membranes. The procedures were followed by periapical radiography, which was repeated three months later. According to the comparative data of the images, the marginal loss of bone tissue was calculated. Both implants were removed to obtain histological specimens. Sections were prepared by standard staining with hematoxylin and eosin. Using an X-ray digitizer, the percentage of implant-to-bone connection was calculated.Results: the data of histological and radiographic studies showed no significant difference in the osseointegration of the implant in the post-extraction lenok and in the mature bone. There was no statistically significant difference in the percentage of bone connection between test and control implants placed in different jaws. Connective tissue in implants is absent, as well as fibrous. Bone destruction was not observed in histological sections.Conclusions: jsseointegration and survival of dental implants without orthopedic load, installed without the use of osteoplastic materials in a fresh post-extraction hole and in a mature bone is almost the same, regardless of the place of installation (upper or lower jaw).
Influence of post-covid hemodynamic disorders on postoperative wound healing process in the maxillofacial region by laser doppler flowmetry
Authors: Grigoreva T. S. , Bezrukov S. G. , Kaladze K. N. 
Published: October 2022

Postoperative wound healing is known to depend on the duration and intensity of local inflammatory and restorative responses. It has been established that the course of the wound process is significantly affected by immunodeficiency states caused by previous diseases, including COVID-19. The article presents an analysis of the results of the study of the active components of microvasculature regulation obtained by laser doppler flowmetry in surgical dental patients after surgical treatment tumor-like formations (epidermoid cysts, atheromas) of the soft tissues of the maxillofacial region. The study was performed at the 1st, 7th and 30th days, as well as at 6 and 12 months after surgery. 98 patients were included in equal groups. Representatives of the main group have a history of COVID-19. It was established that against the background of a surgical wound, the post-covid condition contributes to a significant violation of the functioning of the endothelium of the vascular wall, which is confirmed by significant differences in the decrease in the amplitude of endothelial rhythms in the comparison groups from the first day of the postoperative period. At the same time, a significant increase in the amplitude of myogenic rhythms (Am) on the first day of postoperative LDF-metry in patients in the main group, in comparison with the control (31.67 perf. units versus 23.13 perf. units, respectively), should be interpreted not only as a reaction to surgical injury, but also as a result of long-term complications associated with SARS-CoV-2 virus.Thus, the obtained results give reason to conclude that in surgical dental patients who have a history of COVID-19, the active mechanisms of regulation of the peripheral microvasculature (amplitudes of myogenic and endothelial rhythms) are less stable and, accordingly, are subject to imbalances and pronounced dysfunctions, the correction of which takes a longer time (in comparison with the control). In this regard, patients of the main group need additional perioperative therapy with subsequent rehabilitation.
Self-care agency in pregnancy
Authors: ÖZCAN Handan , ŞAHAN Özlem , GUNAY Mehmet , ŞİMŞEK Hediye 
Published: October 2022

Objective:This study was conducted to evaluate the self-care agency of women during pregnancy.
Methods: A descriptive and cross-sectional design was used in the study. The population of the study consisted of women with pregnancy who presented to obstetrics outpatient clinics in Gumushane public hospital between January 30, 2018 and June 20, 2018, and the sample included women who accepted to voluntarily participate in the study (n=201). Data collection tools included a personal information form and the Self-Care Agency Scale. The data were analyzed on a statistical software package, and the level of significance was determined as p
Development and evaluation of different electroactive poly(vinylidene fluoride) architectures for endothelial cell culture
Authors: Durán-Rey David , Brito-Pereira Ricardo , Ribeiro Clarisse , Ribeiro Sylvie , Sánchez-Margallo Juan A. , Crisóstomo Verónica , Irastorza Igor , Silván Unai , Lanceros-Méndez Senentxu , Sánchez-Margallo Francisco M. 
Published: October 2022

Tissue engineering (TE) aims to develop structures that improve or even replace the biological functions of tissues and organs. Mechanical properties, physical-chemical characteristics, biocompatibility, and biological performance of the materials are essential factors for their applicability in TE. Poly(vinylidene fluoride) (PVDF) is a thermoplastic polymer that exhibits good mechanical properties, high biocompatibility and excellent thermal properties. However, PVDF structuring, and the corresponding processing methods used for its preparation are known to significantly influence these characteristics.In this study, doctor blade, salt-leaching, and electrospinning processing methods were used to produce PVDF-based structures in the form of films, porous membranes, and fiber scaffolds, respectively. These PVDF scaffolds were subjected to a variety of characterizations and analyses, including physicochemical analysis, contact angle measurement, cytotoxicity assessment and cell proliferation.All prepared PVDF scaffolds are characterized by a mechanical response typical of ductile materials. PVDF films displayed mostly vibration modes for the a-phase, while the remaining PVDF samples were characterized by a higher content of electroactive β-phase due the low temperature solvent evaporation during processing. No significant variations have been observed between the different PVDF membranes with respect to the melting transition. In addition, all analysed PVDF samples present a hydrophobic behavior. On the other hand, cytotoxicity assays confirm that cell viability is maintained independently of the architecture and processing method. Finally, all the PVDF samples promote human umbilical vein endothelial cells (HUVECs) proliferation, being higher on the PVDF film and electrospun randomly-oriented membranes. These findings demonstrated the importance of PVDF topography on HUVEC behavior, which can be used for the design of vascular implants.
Periosteum and fascia lata: Are they so different?
Authors: Manon Julie , Evrard Robin , Maistriaux Louis , Fievé Lies , Heller Ugo , Magnin Delphine , Boisson Jean , Kadlub Natacha , Schubert Thomas , Lengelé Benoît , Behets Catherine , Cornu Olivier 
Published: October 2022

Introduction: The human fascia lata (HFL) is used widely in reconstructive surgery in indications other than fracture repair. The goal of this study was to compare microscopic, molecular, and mechanical properties of HFL and periosteum (HP) from a bone tissue engineering perspective.Material and Methods: Cadaveric HP and HFL (N = 4 each) microscopic morphology was characterized using histology and immunohistochemistry (IHC), and the extracellular matrix (ECM) ultrastructure assessed by means of scanning electron microscopy (SEM). DNA, collagen, elastin, glycosaminoglycans, major histocompatibility complex Type 1, and bone morphogenetic protein (BMP) contents were quantified. HP (N = 6) and HFL (N = 11) were submitted to stretch tests.Results: Histology and IHC highlighted similarities (Type I collagen fibers and two-layer organization) but also differences (fiber thickness and compaction and cell type) between both tissues, as confirmed using SEM. The collagen content was statistically higher in HFL than HP (735 vs. 160.2 μg/mg dry weight, respectively, p &lt; 0.0001). On the contrary, DNA content was lower in HFL than HP (404.75 vs. 1,102.2 μg/mg dry weight, respectively, p = 0.0032), as was the immunogenic potential (p = 0.0033). BMP-2 and BMP-7 contents did not differ between both tissues (p = 0.132 and p = 0.699, respectively). HFL supported a significantly higher tension stress than HP.Conclusion: HP and HFL display morphological differences, despite their similar molecular ECM components. The stronger stretching resistance of HFL can specifically be explained by its higher collagen content. However, HFL contains many fewer cells and is less immunogenic than HP, as latter is rich in periosteal stem cells. In conclusion, HFL is likely suitable to replace HP architecture to confer a guide for bone consolidation, with an absence of osteogenicity. This study could pave the way to a bio-engineered periosteum built from HFL.
Automated segmentation of vertebral cortex with 3D U-Net-based deep convolutional neural network
Authors: Li Yang , Yao Qianqian , Yu Haitao , Xie Xiaofeng , Shi Zeren , Li Shanshan , Qiu Hui , Li Changqin , Qin Jian 
Published: October 2022

Objectives: We developed a 3D U-Net-based deep convolutional neural network for the automatic segmentation of the vertebral cortex. The purpose of this study was to evaluate the accuracy of the 3D U-Net deep learning model.Methods: In this study, a fully automated vertebral cortical segmentation method with 3D U-Net was developed, and ten-fold cross-validation was employed. Through data augmentation, we obtained 1,672 3D images of chest CT scans. Segmentation was performed using a conventional image processing method and manually corrected by a senior radiologist to create the gold standard. To compare the segmentation performance, 3D U-Net, Res U-Net, Ki U-Net, and Seg Net were used to segment the vertebral cortex in CT images. The segmentation performance of 3D U-Net and the other three deep learning algorithms was evaluated using DSC, mIoU, MPA, and FPS.Results: The DSC, mIoU, and MPA of 3D U-Net are better than the other three strategies, reaching 0.71 ± 0.03, 0.74 ± 0.08, and 0.83 ± 0.02, respectively, indicating promising automated segmentation results. The FPS is slightly lower than that of Seg Net (23.09 ± 1.26 vs.30.42 ± 3.57).Conclusion: Cortical bone can be effectively segmented based on 3D U-net.
An ultrasensitive detection platform for cocaine: Aptasensing strategy in capillary tube
Authors: Zamanian Javad , Khoshbin Zahra , Hosseinzadeh Hossein , Danesh Noor Mohammd , Khakshour Abdolabadi Ali , Abnous Khalil , Taghdisi Seyed Mohammad 
Published: October 2022

Cocaine as a detrimental addictive drug threats human health through inducing heart problem, blood pressure, anxiety, immunodeficiency, paranoia, and organ damage. Thus, the quantification of cocaine in the biological samples by a simple, high specificity, and fast method is highly urgent to decrease the harmful effect of the misuse of this drug. In this study, we constructed a novel fluorescent aptasensor by combining the fluorescein (FAM)-modified specific aptamer and AuNPs in a capillary tube as the sensing substrate for the first time. The presence of cocaine recovered the fluorescence response of the aptasensor through interaction with the aptamer and differentiation of the aptamer@AuNPs complex. By fluorescence microscopy imaging of the aptasensor substrate and its quantitative analysis, a remarkable linear range from 100 pM to 600 µM and the ultra-low limit of detection (LOD) as 0.31 pM were achieved for the target detection. Cocaine was successfully quantified in the real samples (human serum and urine) by using the aptasensor. The aptasensor is simple, easy-to-use, favorable applicability, and cost-effective; and to the best of our knowledge, it is the first use of the capillary tube as a sensing platform just by using about 3 μl of the samples. It is also an easy-to-carry tool, promising for the on-site target detection. Besides, it can be a portable device for monitoring cocaine by using a handheld single-beam fluorescence microscope. It can be an appropriate detection tool in forensic science and medicine.
Proximal aortic stiffness modifies the relationship between heart rate and backward wave and hence central arterial pulse pressure
Authors: Mthembu Nonhlanhla , Peterson Vernice R. , Norton Gavin R. , Sadiq Eitzaz , Kolkenbeck-Ruh Andrea , Naran Ravi , Yusuf Suraj M. , Tade Grace , Bello Hamza , Bamaiyi Adamu , Libhaber Carlos D. , Dessein Patrick , Peters Ferande , Monareng Taalib , Abdool-Carrim Talib , Cassimjee Ismail , Sareli Pinhas , Modi Girish , Woodiwiss Angela J. 
Published: October 2022

AimsA lower heart rate (HR) increases central blood pressure through enhanced backward wave pressures (Pb). We aimed to determine whether these relationships are modified by increases in aortic stiffness.MethodsUsing non-invasive central pressure, aortic velocity and diameter measurements in the outflow tract (echocardiography), we assessed the impact of aortic stiffness on relationships between HR and arterial wave morphology in 603 community participants &lt; 60 years of age, 221 ≥ 60 years, and in 287 participants with arterial events [stroke and critical limb ischemia (CLI)].ResultsAs compared to community participants &lt; 60 years, those ≥ 60 years or with events had increased multivariate adjusted proximal aortic characteristic impedance (Zc) and carotid femoral pulse wave velocity (PWV) (p &lt; 0.05 to &lt; 0.0001). Community participants ≥ 60 years and those with events also had a greater slope of the inverse relationship between HR and Pb (p &lt; 0.001 for comparison). While in community participants &lt; 60 years, no interaction between indexes of aortic stiffness and HR occurred, in those ≥ 60 years (p &lt; 0.02) and in those with arterial events (p = 0.001), beyond aortic root diameter, an interaction between Zc and HR, but not between PWV and HR independently associated with Pb. This translated into stepwise increases in the slope of HR-Pb relationships at incremental tertiles of Zc. Although HR was inversely associated with the systemic reflection coefficient in community participants ≥ 60 years (p &lt; 0.0001), adjustments for the reflection coefficient failed to modify HR-Pb relations.ConclusionBeyond the impact on systemic wave reflection, increases in proximal aortic stiffness enhance the adverse effects of HR on Pb and hence central BP.
The impact of myocardial fibrosis biomarkers in a heart failure population with atrial fibrillation—The HARVEST-Malmö study
Authors: Nezami Zainu , Holm Hannes , Ohlsson Marcus , Molvin John , Korduner Johan , Bachus Erasmus , Zaghi Amir , Dieden Anna , Platonov Pyotr G. , Jujic Amra , Magnusson Martin 
Published: October 2022

BackgroundSeveral studies suggest that circulating biomarkers of myocardial fibrosis are associated with worse prognosis in subjects with atrial fibrillation (AF). Here, we aimed to explore associations between fibrosis biomarkers, prevalent AF, and left atrial volume (LAV) enlargement in subjects with heart failure (HF). Additionally, we evaluated the prognostic impact of fibrotic biomarkers in HF with co-existing AF.Materials and methodsPatients hospitalized for HF (n = 316, mean age 75 years; 30% women) were screened for AF. Seven proteins previously associated with myocardial fibrosis [metalloproteinase inhibitor 4 (TIMP-4), suppression of tumorigenicity 2 (ST-2), galectin-3 (GAL-3), growth/differentiation factor-15 (GDF-15), and matrix metalloproteinase 2, 3, and 9 (MMP-3, MMP-3, and MMP-9, respectively)] were analyzed using a proximity extension assay. Proteins with significant Bonferroni-corrected associations with mortality and re-hospitalization risk were taken forward to multivariable Cox regression analyses. Further, Bonferroni-corrected multivariable logistic regression models were used to study associations between protein plasma levels, prevalent AF, and severely enlarged left atrial volume index (LAVI ≥ 48 ml/m2).ResultsPrevalent AF was observed in 194 patients at the hospitalization of whom 178 (92%) were re-hospitalized and 111 (57%) died during the follow-up period. In multivariable logistic regression models, increased plasma levels of TIMP-4, GDF-15, and ST-2 were associated with the prevalence of AF, whereas none of the seven proteins showed any significant association with severely enlarged LAVI. Increased plasma levels of five proteins yielded significant associations with all-cause mortality in patients with co-existing AF; TIMP-4 (HR 1.33; CI95% 1.07–1.66; p = 0.010), GDF-15 (HR 1.30; CI95% 1.05–1.62; p = 0.017), GAL-3 (HR 1.29; CI95% 1.03–1.61; p = 0.029), ST-2 (HR 1.48; CI95% 1.18–1.85; p &lt; 0.001), and MMP-3 (HR 1.33; CI95% 1.09–1.63; p = 0.006). None of the proteins showed any significant association with re-hospitalization risk.ConclusionIn this study, we were able to demonstrate that elevated levels of three plasma proteins previously linked to myocardial fibrosis are associated with prevalent AF in a HF population. Additionally, higher levels of five plasma proteins yielded an increased risk of mortality in the HF population with or without co-existing AF.
Impact of levosimendan on efficacy and renal function in acute heart failure according to renal function: A perspective, multi-center, real-world registry
Authors: Zhang Han , Jiang Li , Fu Rui , Qin Ping , Zhang Xuan , Tian Tao , Feng Guang-xun , Yang Yan-min 
Published: October 2022

ObjectiveAcute heart failure (AHF) is associated with high mortality. Levosimendan, an inodilator, has proved to increase cardiac output and exert renoprotective effect in AHF. Our aim was to investigate the efficacy and renoprotective effects of levosimendan in patients with AHF and different renal function.MethodsThis is a prospective, observational, multi-center registry. Patients admitted with AHF between June 2020 and May 2022 and treated with levosimendan during the hospital stay were included. Baseline characteristics, laboratory tests, electrocardiogram (ECG), chest X-ray, echocardiography, and treatment were collected. A 5-point Likert scale was used to document patients' baseline dyspnea. The estimated glomerular filtration rate (eGFR) was calculated by means of the Modification of Diet in Renal Disease equation. After levosimendan infusion, patients underwent assessment of degree of dyspnea, and levels of brain-type natriuretic peptide (BNP) /N-terminal pro-BNP (NT-pro BNP), and eGFR repeatedly.ResultsAmong 789 AHF patients who received levosimendan treatment in this study, 33.0 % were female, mean age was 64.9 ± 16.8 years, and mean eGFR was 72.6 ± 32.5 ml/min/m2. The mean score of dyspnea was 3.0 ± 1.0 using 5-point Likert scale before levosimendan infusion. Dyspnea improved in 68.7% patients at 6h after infusion of levosimendan, and in 79.5% at 24 h. Lower eGFR was associated with lower efficacy rate after 6h infusion (71.7, 70.7, 65.2, and 66.0%, respectively) and after 24 h infusion (80.5, 81.4, 76.2, and 77.8%, respectively). The levels of BNP or NT-pro BNP were also decreased after levosimendan treatment, and in each eGFR category. Levels of eGFR increased from baseline (72.6 ± 32.5 ml/min/m2) to 12–24h (73.8 ± 33.5 ml/min/m2) and 24–72h (75.0 ± 33.4 ml/min/m2) after starting treatment (p &lt; 0.001). However, the eGFR levels increased only in patients with eGFR lower than 90.0 ml/min/m2.ConclusionsIn AHF patients who received levosimendan, degree of dyspnea and levels of BNP or NT-pro BNP were significantly improved, especially in patients with higher eGFR levels. However, levosimendan infusion increase eGFR only in AHF patients with renal dysfunction.