bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Different Proteins Binding to Na, K-ATPase α1 in LPS-Induced ARDS Cell Model by Proteomic Analysis
Authors: Wen Xu-Peng , Long Guo , Zhang Yue-Zhong , Huang He , Liu Tao-Hua , Wan Qi-Quan 
Published: January 2022

Abstract
Background: Acute respiratory distress syndrome (ARDS) is characterized by refractory hypoxemia caused by accumulation of pulmonary fluid, which is related to inflammatory cell infiltration, impaired tight junction of pulmonary epithelium and impaired Na, K-ATPase function, especially Na, K-ATPase α1 subunit. Up until now, the pathogenic mechanism at the level of protein during lipopolysaccharide- (LPS-) induced ARDS remains unclear.Methods: Using an unbiased, discovery and quantitative proteomic approach, we discovered the differentially expressed proteins binding to Na, K-ATPase α1 between LPS-A549 cells and Control-A549 cells. These Na, K-ATPase α1 interacting proteins were screened by co-immunoprecipitation (Co-IP) technology. Among them, some of the differentially expressed proteins with significant performance were identified and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The protein interaction network was constructed by the related Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Several differentially expressed proteins were validated by Western blot.Results: Of identified 1598 proteins, 89 were differentially expressed proteins between LPS-A549 cells and Control-A549 cells. Intriguingly, protein-protein interaction network showed that there were 244 significantly enriched co-expression among 60 proteins in the group control-A549. while the group LPS-A549 showed 43 significant enriched interactions among 29 proteins. The related GO and KEGG analysis found evident phenomena of ubiquitination and deubiquitination, as well as the pathways related to autophagy. Among proteins with rich abundance, there were several intriguing ones, including the deubiquitinase (OTUB1), the tight junction protein zonula occludens-1 (ZO-1), the scaffold protein in CUL4B-RING ubiquitin ligase (CRL4B) complexes (CUL4B) and the autophagy-related protein sequestosome-1 (SQSTM1).Conclusions: In conclusion, our proteomic approach revealed targets related to the occurrence and development of ARDS, being the first study to investigate significant differences in Na, K-ATPase α1 interacting proteins between LPS-induced ARDS cell model and control-A549 cell. These proteins may help the clinical diagnosis and facilitate the personalized treatment of ARDS.
Analysis and Study of Flow Patterns and Sediment Deposition in the Forebay of a Forward Intake Pumping Station
Authors: WANG Haidong , LI Chunguang , LV Suiju , SONG Lulu 
Published: January 2022

Abstract
In Northwest China, the sediment concentration of the Yellow River is high. A project to investigate the operation of a pumping station shows that the flow patterns in the forebay and inlet tank are disordered, and there is sediment deposition that endangers the normal operation and safety of the pumping station. To solve this problem, the three-dimensional two-phase water-sediment flow in the forebay of the pumping station is modeled by using fluid simulation software, and diagrams of the sediment volume fraction content and vector distribution in the flow layers of different sections are obtained. Combined with the multiphase flow theory of mixtures and the realizable turbulent kinetic energy equation, the location and formation mechanism of each vortex, as well as the area and degree of sediment deposition in the forebay, are analyzed. The actual engineering and numerical simulation results are compared to verify the accuracy of the simulation. The results show that the main reason for sediment deposition is the high sediment concentration of the Yellow River, but the flow pattern disorder is affected by a specific design defect of the forebay, which makes the sediment deposition worse. The results of this study provide specific guidance and methods for the construction and transformation of the forebay of the pump station in the future; construction to weaken the return area to a certain extent can reduce the degree of sedimentation.
Optimization of the Process Parameters for Micro-Milling Thin-Walled Micro Parts Using Advanced Algorithms
Authors: Wang Peng , Bai Qingshun , Cheng Kai , Zhao Liang , Ding Hui 
Published: January 2022

Abstract
The surface integrity and machining accuracy of thin-walled micro parts are significantly affected by micro-milling parameters mostly because of their weak stiffness. Furthermore, there is still a lack of studies focusing on parameters optimization for the fabrication of thin-walled microscale parts. In this paper, an innovative approach is proposed for the optimization of machining parameters with the objectives of surface quality and dimension accuracy, which integrates the Taguchi method, principal component analysis method (PCA) and the Non-dominated sorting genetic algorithm (NSGA-II). In the study, surface arithmetic average height Sa, surface root mean square height Sq, and 3-D fractal dimension Ds are selected to evaluate surface quality. Then micro-milling experiments are conducted based on the Taguchi method. According to the experimental results, the significance of machining parameters can be determined by range analysis. Besides, regression models for the responses are developed comparatively, and the PCA method is employed for dimension reduction of the optimization objective space. Finally, two combinations of machining parameters with the highest satisfaction are obtained through NSGA-II, and verification experiments are carried out. The results show that the surface quality and dimension accuracy of the thin-walled microscale parts can be simultaneously improved by using the proposed approach.
A Novel Geopolymer Foam Based On ZSM-5 Zeolite Fabricated Using Templating Emulsion/Chemical Foaming Method and Its Application in Batch and Continuous Dye Removal Systems
Authors: Monjezi Mahboobeh , Javanbakht Vahid 
Published: January 2022

Abstract
Geopolymers as sustainable and environmentally friendly “green materials”, can be synthesized by utilizing waste material and by-products. A porous geopolymer foam adsorbent based on ZSM-5 zeolite was prepared using templating emulsion/chemical foaming method in different conditions and used for dye removal in batch and continuous systems. The parameters affecting the dye adsorption including temperature, concentration, and pH, kinetics, isotherm, and thermodynamics of the process were investigated. The results of the geopolymer foam synthesis showed that thermal pretreatment of the zeolite has a positive effect on the strength and adsorption capacity. Moreover, the increase in sodium silicate more than the stoichiometric reduces the strength and adsorption capacity. The findings obtained from the batch adsorption process showed that the adsorption kinetics of the pseudo-second-order model and the adsorption isotherm of the Temkin model is adjusted with the experimental data. Thermodynamic results indicated that the process of dye adsorption with geopolymer foam is exothermic. The results from continuous experiments indicated more compatibility of the adsorption process with the models of Thomas and Bohart-Adams. The maximum adsorption capacity of methylene blue in batch and continuous processes was 9.82 and 8.17 mg/g. The adsorbent reduction was performed successfully by chemical and thermal processes.
Biomarkers of Length of Stay On An Inpatient Eating Disorder Unit For Treatment of Anorexia Nervosa in Females
Authors: Breiner Courtney E. , Qi Baiyu , Thornton Laura M. , Brownley Kimberly A. , Foreman Tonya , Bardone-Cone Anna M. , Bulik Cynthia M. , Baker Jessica H. 
Published: January 2022

Abstract
Background. Length of stay on an inpatient unit for treatment of anorexia nervosa (AN) is widely variable. Although previous research has used anthropometric and clinical variables and duration of illness to predict length of stay, there has been limited investigation of the predictive ability of biomarkers. Biomarkers, including those collected through a comprehensive metabolic panel (CMP) and appetite hormones, such as ghrelin and leptin, are impacted by disease presence and may play an etiological role in AN. Methods. Using a series of regression models, we retrospectively evaluated the associations of these putative biomarkers at admission with length of inpatient stay in 59 females receiving treatment on an inpatient eating disorder unit for anorexia nervosa. Results. Both lower levels of magnesium and higher active ghrelin levels at inpatient admission predicted length of stay. Conclusions. This research provides further evidence supporting both biological and psychological components of AN, identifying potential biomarkers that could aid in prospective prediction of treatment needs. Ghrelin monitoring throughout inpatient stays may aid clinicians in better predicting physical recovery and renourishment from AN and prepare for stepdown from an inpatient setting. Further research is necessary to replicate and extend these findings across treatment settings.
Translation and Validation of the Hungarian Version of the Infection Control Standardized Questionnaire: A Cross-sectional Study
Authors: Hammoud Sahar , Amer Faten , Khatatbeh Haitham , Alfatafta Huda , Zrínyi Miklós , Kocsis Béla 
Published: January 2022

Abstract
Background: Up to our knowledge, there is currently no psychometrically validated Hungarian scale to evaluate nurses’ knowledge about infection prevention and control (IPC) practices. Thus, we aim in this study to assess the validity and reliability of the infection control standardized questionnaire Hungarian version (ICSQ-H).Methods: A cross-sectional, multisite study was conducted among 591 nurses in Hungary. The original ICSQ including 25 items was translated into Hungarian. A panel of four experts assessed the content validity of the questionnaire by calculating the item content validity index and scale content validity index. Then, construct validity was evaluated using principal component analysis and confirmatory factor analysis. The goodness of fit for the model was measured through fit indices. Convergent validity was assessed by calculating the average variance extracted. Additionally, discriminant validity was evaluated by computing the spearman correlation coefficient between the constructs. Finally, the interitem correlations, the corrected item-total correlations, and the internal consistency were calculated.Results: Content validity of the questionnaire was established with 23 items. The final four-construct ICSQ-H including 10 items showed a good fit model. Convergent validity was met except for the alcohol-based hand rub (ABHR) construct, while discriminant validity was met for all constructs. The interitem correlations and the corrected item-total correlations were met for all constructs but, the internal consistency of ABHR was unsatisfactory due to the low number of items.Conclusions: The results did not support the original three-factor structure of the ICSQ. However, the four-factor ICSQ-H demonstrated an adequate degree of good fit and was found to be reliable. Based on our findings, we believe that the ICSQ-H could pave the way for more research regarding nurses’ IPC knowledge to be conducted in Hungary. Nevertheless, its validation among other healthcare workers is important to tailor effective interventions to enhance knowledge and awareness.
The bows and rings of dawn and dusk
Authors: Cruz Gerson Kniphoff da 
Published: January 2022

Abstract
The arcs of dawn and dusk are natural phenomena that define the boundary of the border between day and night. They are associated with the refraction of solar rays at high angles of incidence that converge to project an arc of light onto the back side of the earth's atmosphere. The rings of dawn and dusk, in turn, are associated with rays, also at large angles of incidence, which converge to project the image of the Sun. Arcs and rings become visible by scattering light by clouds or particles suspended in atmospheric air in the region in which they occur. Here we show a model that describes these natural phenomena and report the first-time record image produced in July of this year.
Performance Investigation of Routing Protocol with the velocity of 30 m/s for Random Mobility Model
Authors: Vyas Ajay Kumar , Suthar Margam 
Published: January 2022

Abstract
A mobile ad-hoc network (MANET) is a network of mobile nodes short of Infrastructure, linked by wireless links. While mobility is the key feature of MANETs, the frequent movement of nodes may lead to link failure. A mobile multi-hop wireless ad hoc network carries a dynamic structure feature, and each node has mobility; due to this, the network has altered topology change dynamically. Developing the wireless ad hoc network protocol is the major challenge because, compared to the wired routing node, all node is mobile, energy limitation, the node's physical location, and multicast routing. In this article, a comparative investigation of routing protocol performance for large wireless ad hoc networks (100 nodes) under the impact of the random mobile environment with the velocity of 30 m/sec for 1800 seconds with ten different results for each node-set. The comparative analysis includes packet delivery ratio, throughput, packet dropping ratio, routing overhead, and end-to-end delay quality of service (QoS) metrics. It concludes that Ad-hoc On-demand Distance Vector protocol performance is more stable as the number of nodes & traffic increase in the random mobility environment.
Enhanced osseointegration of dental implants with reduced graphene oxide coating
Authors: Shin Yong Cheol , Bae Ji-Hyeon , Lee Jong Ho , Raja Iruthayapandi Selestin , Kang Moon Sung , Kim Bongju , Hong Suck Won , Huh Jung-Bo , Han Dong-Wook 
Published: January 2022

Abstract
Background: The implants of pure titanium (Ti) and its alloys can lead to implant failure because of their poor interaction with bone-associated cells during bone regeneration. Surface modification over implants has achieved successful implants for enhanced osseointegration.Methods: Herein, we prepared sandblasted, large-grit, and acid-etched (SLA) Ti (ST) implants with different surface modifications [i.e., reduced graphene oxide (rGO) and recombinant human bone morphogenetic protein-2 (rhBMP-2)] and investigated their dental tissue regenerating ability in animal models. We performed comparative studies in surface property, in vitro cellular behaviors, and in vivo osseointegration activity among different groups, including ST (control), rhBMP-2-immobilized ST (BI-ST), rhBMP-2-treated ST (BT-ST), and rGO-coated ST (R-ST).Results: Spectroscopic, diffractometric, and microscopic analyses confirmed that rGO was coated well around the surfaces of Ti discs (for cell study) and implant fixtures (for animal study). Furthermore, in vitro and in vivo studies revealed that the R-ST group showed significantly better effects in cell attachment and proliferation, alkaline phosphatase activity, matrix mineralization, and osseointegration than the control (ST), BI-ST, and BT-ST groups.Conclusion: Hence, we suggest that the rGO-coated Ti can be a promising candidate for the application to dental or even orthopedic implants due to its ability to accelerate the healing rate with the high potential of osseointegration.
SLC6A14 impacts cystic fibrosis lung disease severity via mTOR and epithelial repair modulation
Authors: Mercier Julia , Calmel Claire , Mésinèle Julie , Sutanto Erika , Merabtene Fatiha , Longchampt Elisabeth , Sage Edouard , Kicic Anthony , Boëlle Pierre-Yves , Corvol Harriet , Ruffin Manon , Guillot Loic 
Published: January 2022

Abstract
Cystic fibrosis (CF), due to variants in CFTR gene, is associated with chronic infection/inflammation responsible for airway epithelium alteration and lung function decline. Modifier genes induce phenotype variability between people with CF (pwCF) carrying the same CFTR variants. Among these, the gene encoding for the amino acid transporter SLC6A14 has been associated with lung disease severity and age of primary airway infection by the bacteria Pseudomonas aeruginosa. In this study, we investigated whether the single nucleotide polymorphism (SNP) rs3788766, located within SLC6A14 promoter, is associated with lung disease severity in a large French cohort of pwCF. We also studied the consequences of this SNP on SLC6A14 promoter activity using a luciferase reporter and the role of SLC6A14 in mammalian target of rapamycin (mTOR) signaling pathway and airway epithelial repair. We confirm that SLC6A14 rs3788766 SNP is associated with lung disease severity in pwCF (p=0.020; n=3,257, pancreatic insufficient, aged 6 to 40 years old), with the minor allele G being deleterious. In bronchial epithelial cell lines deficient for CFTR, SLC6A14 promoter activity is reduced in the presence of the rs3788766 G allele. SLC6A14 inhibition with a specific pharmacological blocker reduced 3H-arginine transport, mTOR phosphorylation and bronchial epithelial repair rates in wound healing assays. To conclude, our study highlights that SLC6A14 genotype might affect lung disease severity of people with cystic fibrosis via mTOR and epithelial repair mechanisms modulation in the lung.
Probabilistic Modelling of Sustainable Urban Drainage Systems
Authors: Raimondi Anita , Chiano Maria Gloria Di , Marchioni Mariana , Sanfilippo Umberto , Becciu Gianfranco 
Published: January 2022

Abstract
Sustainable Urban Drainage Systems (SuDS) gatherer effective strategies and control systems for stormwater management especially in highly urbanized areas characterized by large impervious surfaces that increase runoff peak flow and volume. The main goal is to restore the natural water balance by increasing infiltration, evapotranspiration and promoting rainwater reuse. This paper proposes an analytical probabilistic approach for the modelling SuDS applicable to different structures and goals. Developed equations allow to estimate the probability of overflow and the probability of pre-filling at the end of dry periods, to evaluate the efficiency of the storage in rainwater management and its ability to empty between consecutive events. A great advantage of the proposed method is that it allows to consider a chain of rainfall events; this aspect is particularly important for control systems SuDS characterized by low outflow rates which storage capacity is often not completely available at the end of a dry period because pre-filled by previous events. Suggested formulas were tested to two cases studies in Milan and Genoa, Italy.
Assessment of correlation amongst physico-chemical, topographical, geological, lithological and soil type parameters for measuring water quality of Rawal watershed using remote sensing
Authors: Ahmed Mehreen , Mumtaz Rafia , Baig Shahbaz , Zaidi Syed Muhammad Hassan 
Published: January 2022

Abstract
The quality of water is traditionally assessed by the collection of physico-chemical parameters, i.e., pH, turbidity, dissolved oxygen of the water bodies. However, the variations in environmental factors may have an impact on the quality of water, as changes in these attributes may affect the water bodies. These factors include the topographical, geological, lithological and soil type parameters of the watershed. In this study, the relationship amongst the physico-chemical, topographical, geological, lithological and soil type parameters of the Rawal watershed was evaluated. The parameters included in the present study could be classified as follows: (a) water quality parameters (b) topographical parameters, (c) geological parameters, (d) lithological parameters, and (e) soil type parameters. Water quality parameters consisted of dissolved oxygen, pH, turbidity and temperature. The topographical parameters include the slope and aspect of the watershed while the lithological, geological and soil type parameters include the lithology, geology and soil type of the watershed. Pearson's correlation was used to determine the relationship amongst these different parameters. The results have revealed that the correlations of the topographical, lithological, geological parameters with the water quality parameters in the Rawal watershed for the monsoon seasons of June to August mostly have the same trend. Throughout the four-year time period, turbidity and temperature parameters had positive correlations with soil type (ranging 0.03–0.24), but however had weak correlation with geological and lithological parameters. Dissolved oxygen did not show any relationship with topographical and lithological parameters. The results for pH show that it has weak to fair positive correlations with topographical parameters. However, this analysis is based on the Landsat 8 images extracted for the monsoon seasons of the years 2017–2020, and to examine a more prominent impact of geographical or environmental factors on the physico-chemical features, a large dataset should be considered.
Operatywno-heurystyczne kształcenie dla zdrowia w koncepcji filozoficznej Juliana Aleksandrowicza
Authors: Jagiełłowicz Alina Bernadetta 
Published: January 2022

W tekście podjęto zagadnienie wartości operatywno-heurystycznego prozdrowotnego kształtowania postaw inspirowanego filozoficzną koncepcją Juliana Aleksandrowicza. Tło rozważań stanowią obecne we współczesnej kulturze tendencje rozwoju ultratechnologii. Z uwagi na odcięcie się medycyny od korzenia filozofii zachodzi konieczność przewartościowania dziedzictwa neopozytywistycznego oraz redefinicji pojęć: „zdrowie” i „choroba”. Problem decyzyjny „życie albo śmierć”, definiowany w kategoriach dysjunkcji, narzuca nowy sposób interpretacji pojęć moralnych. Idea autokreacji w procesie przywracania dobrze stworzonej natury jest w tej koncepcji równoważona przez subiektywne poczucie zdrowia, odnoszone do szerokiego kontekstu fizycznego i psycho-duchowego, społecznego, środowiskowego. Myśl ta znalazła odzwierciedlenie w przyjętej przez Juliana Aleksandrowicza koncepcji holistycznie ujmowanego zdrowia jako wartości moralnej.
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Zgłoszono: 21/04/2021. Zrecenzowano: 14/06/2021. Zaakceptowano do publikacji: 06/09/2021.
W poszukiwaniu tożsamości współczesnej filozofii przyrody
Authors: Janowski Jarosław Maciej 
Published: January 2022

W artykule podjęto kwestię dotyczącą tożsamości filozofii przyrody w kontekście trzech współcześnie wymienianych charakterystycznych cech tej dyscypliny, a mianowicie jej różnorodności metodologicznej, interdyscyplinarności oraz swoistej dynamiki rozwojowej. Analizowane są zagrożenia dla tożsamości filozofii przyrody, wynikające z takiego zestawu cech, w szczególności z różnorodności metodologicznej filozofii przyrody. Jednocześnie podjęto próbę obrony wymienionych cech jako powodujących nie tylko zagrożenia, ale również (paradoksalnie) wzmacniających tożsamość filozofii przyrody. Całość analiz odnosi się do metafilozoficznej charakterystyki dziedziny filozofii.
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Zgłoszono: 13/08/2021. Zrecenzowano: 06/09/2021. Zaakceptowano do publikacji: 14/10/2021.