bluuebunny/crossref_metadata_2025_split huggingface dataset:
From an Al4-Cluster to Al+ Complexes: Lessons on Metalloidal Clustering
Authors: Dabringhaus Philipp , Willrett Julie , Krossing Ingo 
Published: January 2022

Abstract
Low-valent aluminium compounds are among the most reactive and widely researched main-group compounds. Since the isolation of [(AlCp*)4] in 1991 as the first stable, molecular AlI compound, a variety of highly reactive neutral or anionic low-valent aluminium complexes were developed. In particular, the strongly basic aluminyl anions allowed for nucleophilic activation of a large variety of small molecules and formation of elusive transition-metal complexes. By contrast, an accessible cationic, low-valent aluminium compound combining the nucleophilicity of low-valent compounds with the electrophilicity of aluminium is hitherto unknown. Here, we report the synthesis of [Al(AlCp*)3]+[Al(ORF)4]– (RF = C(CF3)3) via a simple metathesis route. Unexpectedly, the complex ion forms a dimer in the solid state and in concentrated solutions. Addition of Lewis bases results in monomerization and coordination to the unique formal Al+ atom giving [(L)xAl(AlCp*)3]+ salts with L = hexaphenylcarbodiphosporane (cdp; x = 1), tetramethylethylenediamine (tmeda; x = 1) and 4-dimethylamino-pyridine (dmap; x = 3). Depending on the donor strength of the ligand added, the Al+–AlCp* bonds in the [(L)xAl(AlCp*)3]+ cluster cations can be finely tuned between very strong (L = nothing) to very weak and approaching isolated [Al(L)3]+ ions (L = dmap). We anticipate our easily accessible low-valent aluminium cation salts to be the starting point for investigation and potential application of this unusual compound class. In particular, the ambiphilic reactivity of the cationic, low-valent compounds will be studied. Moreover, knowledge gained from the stabilization of the reported complex salts is expected to facilitate the isolation and application of novel cationic, low-valent Al complexes.
Draft Genome of Tanacetum Coccineum: Genomic Comparison of Closely Related Tanacetum-Family Plants
Authors: Yamashiro Takanori , Shiraishi Akira , Nakayama Koji , Satake Honoo 
Published: January 2022

Abstract
The plant Tanacetum coccineum (painted daisy) is closely related to Tanacetum cinerariifolium (pyrethrum daisy). However, T. cinerariifolium produces large amounts of pyrethrins, a class of natural insecticides, whereas T. coccineum produces much smaller amounts of these compounds. Thus, comparative genomic analysis is expected to contribute a great deal to investigating the difference in biological defense systems, including pyrethrin biosynthesis. Here, we elucidated the 9.4-Gb draft genome of T. coccineum, consisting of 2,836,647 scaffolds and 103,680 genes. Comparative analyses of the draft genome of T. coccineum and that of T. cinerariifolium, generated in our previous study, revealed distinct features of T. coccineum genes. While the T. coccineum genome contains more numerous ribosome-inactivating protein (RIP)-encoding genes, the number of higher-toxicity type-II RIP-encoding genes is larger in T. cinerariifolium. Furthermore, the number of histidine kinases encoded by the T. coccineum genome is smaller than that of T. cinerariifolium, suggesting a biological correlation with pyrethrin biosynthesis. Moreover, the flanking regions of pyrethrin biosynthesis-related genes are also distinct between these two plants. These results provide clues to elucidation of species-specific biodefense systems, including the regulatory mechanisms underlying pyrethrin production.
Celiac Disease in Autism Spectrum Disorder: Data from an Italian Child Cohort
Authors: Zambrano Stefania , Parma Barbara , Morabito Valeria , Borini Silvia , Romaniello Roberta , Molteni Massimo , Mani Elisa , Selicorni Angelo 
Published: January 2022

Abstract
Background: In recent decades some studies described the frequent co-occurrence of celiac disease autoimmunity and/or overt celiac disease in patients with autism. Therefore, it was suggested that celiac disease could play a possible role in the etiopathogenesis of autism spectrum disorder. However, several other studies have not confirmed this association. The aim of the present study was to elucidate the potential association between autism spectrum disorder and celiac disease.Methods: We prospectively collected data from an Italian cohort of 223 children at the time of their clinical diagnosis of autism spectrum disorder in the 2019-2020 period. A serological celiac disease screening was performed and data were available for 196 patients; male (M):female (F) ratio = 4.4:1; median age = 3.6 years; age range = 1.6–12.8 years. Full-blown celiac disease was established according to the diagnostic algorithm of the European Society of Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) 2012 or 2019 guidelines. Fisher’s exact test was used to compare the celiac disease seroprevalence and prevalence in our autism spectrum disorder cohort and in the Italian healthy pediatric population studied by Gatti et al. to highlight the possible differences between the two groups.Results: A not statistically significant difference between the celiac disease seroprevalence in our autism spectrum disorder cohort (4.08%) and Gatti’s Italian healthy group (2.22%) was found, p = 0.0810; OR = 1.871. A similar result emerged for overt celiac disease prevalences (2.24% versus 1.58%, respectively), p = 0.2862; OR = 1.431.Conclusions: Our data validates a weakness of association between autism spectrum disorder and celiac disease. Regular screening for celiac disease in young patients with autism spectrum disorder is not strongly recommended to a greater extent than in the general population.
The COVID-19 pandemic impacted maternal mental health differently depending on pregnancy status and trimester of gestation
Authors: Bérard Anick , Gorgui Jessica , Tchuente Vanina , Lacasse Anaïs , Gomez Yessica-Haydee , Côté Sylvana , King Suzanne , Muanda Flory , Mufike Yves Mufike , Boucoiran Isabelle , Nuyt Anne Monique , Quach Caroline , Ferreira Ema , Kaul Padma , Winquist Brandace , O'Donnell Kiernan , Eltonsy Sherif , chateau Dan , Zhao Jinping , Hanley Gillian , Oberlander Tim , Kassai Behrouz , Mainbourg Sabine , Bernatsky Sasha , Vinet Evelyne , Brodeur-doucet Annie , Demers Jackie , Richebé Philippe , Zaphiratos Valerie 
Published: January 2022

Abstract
Introduction: We aimed to measure the impact of the COVID-19 pandemic on maternal mental health, stratifying on pregnancy status, trimester of gestation, and pandemic period/wave.Methods: Pregnant persons and persons who delivered in Canada during the pandemic, >18 years, were recruited, and data were collected using a web-based strategy. The current analysis includes data on persons enrolled between 06/2020-08/2021. Maternal sociodemographic indicators, mental health measures (Edinburgh Perinatal Depression Scale (EPDS), Generalized Anxiety Disorders (GAD-7), stress) were self-reported. Maternal mental health in pregnant women (stratified by trimester, and pandemic period/wave at recruitment) was compared with mental health of women who had delivered; determinants of severe depression were identified with multivariate logistic regression models.Results: 2,574 persons were pregnant and 626 had already delivered at recruitment. Participants who had delivered had significantly higher mean depressive symptom scores compared to those pregnant at recruitment (9.1 (SD, 5.7) vs. 8.4 (SD, 5.3), p=0.009). Among those who were pregnant at recruitment, depressive symptoms were significantly higher in women recruited in their third trimester, and those recruited during the 2nd wave of the pandemic. Maternal anxiety (aOR 1.51; 95%CI 1.44-1.59) and stress (aOR 1.35; 95%CI 1.24-1.48) were the most significant predictors of severe maternal depression (EDPS˃13) in pregnancy. Conclusion: The COVID-19 pandemic had a significant impact on maternal depression during pregnancy and in the post-partum period. Given that gestational depression/anxiety/stress have been associated with preterm birth and childhood cognitive problems, it is essential to continue following women/children, and develop strategies to reduce COVID-19’s longer-term impact.
Disparities In Eye Clinic Patient Encounters Among Patients Requiring Language Interpreter Services
Authors: Mudie Lucy I. , Patnaik Jennifer L. , Gill Zafar , Wagner Marissa , Christopher Karen L. , Seibold Leonard K. , Ifantides Cristos 
Published: January 2022

Abstract
Background: Communication barriers are a major cause of health disparities for patients with limited English proficiency. Medical interpreters play an important role in bridging this gap, however the impact of interpreters on outpatient eye center visits has not been studied. We aimed to evaluate the impact of medical interpreters on eyecare patient encounters at a tertiary, safety-net hospital in the United States.Methods: A retrospective review of patient encounter metrics collected by our electronic medical record was conducted for all encounters between January 1, 2016 and December 31, 2020. Patient demographics, primary language spoken, need for interpreter and encounter characteristics including new patient status, patient time waiting for providers and time in room were collected. We compared these patient visit times by interpreter use, with our main outcomes being time spent with ophthalmic technician, time spent with eye care provider, and time waiting for eye care provider. Interpreter services at our hospital are typically remote (via phone or video). Results: A total of 202,895 patient encounters, of which 56,230 (27.7%) required an interpreter, were analyzed. After adjusting for patient age at visit and new patient status, patients requiring an interpreter spent between 1.7 to 4.5 minutes longer with the technician and 1.1 to 2.1 minutes with their ophthalmologist (p<0.01), dependent on language. Spanish speakers also spent on average 1.2 minutes longer waiting for their providers after the technician completely their work up than English speakers (p<0.01), but for other languages this difference was not significant. Limited English Proficiency patients with an interpreter needed were more likely to keep their appointment once it was made when compared to those patients not needing an interpreter, and more likely to have their allergies, problem list and medications reviewed during their visit. Conclusions: Interpreters have a statistically significant impact on the eye clinic patient encounter, but less than we expected. Eyecare providers must be aware of this to prevent negative impacts on patient care. Equally important, healthcare systems should consider ways to prevent unreimbursed extra time from being a financial disincentive for seeing patients who require interpreter services.
Wellens’ Syndrome: Incidence, Characteristics, and Long-Term Clinical Outcomes
Authors: Zhou Li , Gong Xuhe , Dong Tianhui , Cui He-he , Chen Hui , Li Hongwei 
Published: January 2022

Abstract
Background: Sparse study of large sample size are available on patients with Wellens’ syndrome. We sought to assess the current incidence, risk factors, clinical presentation and long-term outcomes of this population. Methods: Among a total of 3528 patients with ACS underwent angioplasty from 2017 to 2019 in our center, 2127 NSTE-ACS patients with culprit vessel of LAD were enrolled in this study. According to electrocardiographic criteria, they were divided into Wellens’ group (n = 200) and non-Wellens’ group (n =1927). The primary endpoint was cardiac death; the secondary endpoint was MACCE, a composite of all-cause death, cardiac death, recurrent myocardial infarction, target lesion revascularization, heart failure and stroke. Results: The incidence of Wellens’ syndrome was 5.7% (200 of 3528) in all the ACS patients. Wellens’ syndrome was more often manifested as NSTEMI (69% vs 17.5%, P＜0.001). Percent of preexisting coronary heart disease (39.6% vs 23%) and previous PCI (19.5% vs 9%) were significantly higher in the non-Wellens’ group than in Wellens’ group (all P<0.001). More importantly, the proportion of early PCI was higher in Wellens’ group (68% vs 59.3%, P=0.017). At a median follow-up of 24 months, Wellens’ syndrome was not a factor that affects the prognosis of MACCE (P=0.05) and cardiac death (P=0.188). Conclusions: In patients with NSTE-ACS, Wellens’ syndrome does not affect the prognosis. The presence of age≥65years, diabetes, NSTEMI, eGFR< 60ml/min and left main disease were associated with an incidence of cardiac death. Early recognition and aggressive intervention are critical as they may help to attenuate adverse outcomes.
Manipulator Trajectory Control Combined with Moving Average Filtering Algorithm
Authors: Meng Jian-Jun , Chen Xiao-Tong , Qi Wen-Zhe , Li De-Cang , Xu Ru-Xun 
Published: January 2022

Abstract
To solve the problem of abnormal angular velocity and angular acceleration in manipulator trajectory motion controlled by quintic spline interpolation algorithm, a manipulator trajectory control algorithm combined with moving average filtering algorithm was proposed. Based on the quintic spline interpolation algorithm, the moving average filtering algorithm was used to clean the abnormal data under the quintic spline interpolation. And the recursive forward dynamics model based on joint space motion was used to design the trajectory motion control of the manipulator. The simulation results show that the manipulator trajectory control algorithm combined with the moving average filtering algorithm has strong constraint ability of diagonal velocity and angular acceleration, and 67% of the maximum velocity and maximum acceleration of the joint axis of the designed manipulator trajectory are significantly reduced, and the curve is smoother.
Alcohol Consumption and Closed Borders - How COVID-19 Restrictions have Impacted Alcohol Sales and Consumption in Europe
Authors: Leifman Håkan , Dramstad Kalle , Juslin Emil 
Published: January 2022

Abstract
BackgroundThe closing of bars, restaurants and international borders during the COVID-19 pandemic led to significant changes in alcohol availability. The study provides a first systematic overview of the monthly development of alcohol sales in Europe during the pandemic in order to determine the effect of closed borders on the sales and consumption of alcohol.MethodsThe study covers 60 months from January 2015 to December 2020 in 14 northern-European countries with excise revenue data for beer, wine, spirits separately and summed, converted into litres of pure alcohol per capita 15+ as a proxy for alcohol sales. March-December 2020 is seen as the pandemic period. The analyses consist of (1) descriptive trends of sales before and during the pandemic, (2) assessment of the pandemic impact on sales by time-series analyses and (3) case studies of countries with substantial cross-border inflow or outflow of alcohol.ResultsThe result shows an overall reduction in alcohol sales during the pandemic. Nevertheless, the results differ based on the level of cross-border purchasing flows pre-pandemic, as countries with high cross-border inflow saw an increase in domestic sales as the pandemic hit. ConclusionThe closing of intra-European borders had a significant redistributing effect on alcohol sales. While noting sales increases, cross-border inflow countries generally saw a decrease in total alcohol consumption as not all cross-border purchases were replaced by domestic sales. This has important policy implications as large volumes of cross-border inflow of alcohol can negatively affect excise revenue as well as public health outcomes. The methodology can be used to further explore the reliance of different purchasing streams in a domestic alcohol market.
Cerebellar Engagement In The Attachment Behavioral System
Authors: Picerni Eleonora , Laricchiuta Daniela , Piras Fabrizio , Petrosini Laura , Spalletta Gianfranco , Cutuli Debora 
Published: January 2022

Abstract
Brain structural bases of individual differences in attachment are not yet fully clarified. Given the evidence of relevant cerebellar contribution to cognitive, affective, and social functions, the present research was aimed at investigating potential associations between attachment dimensions (through the Attachment Style Questionnaire, ASQ) and cerebellar macro- and micro-structural measures (Volumetric and Diffusion Tensor Imaging data). In a sample of 79 healthy subjects, cerebellar and neocortical volumetric data were correlated with ASQ scores at the voxel level within specific Regions Of Interest. Also, correlations between ASQ scores and age, years of education, anxiety and depression levels were performed to control for the effects of sociodemographic and psychological variables on neuroimaging results.Positive associations between scores of the Preoccupation with Relationships (ASQ subscale associated to insecure/anxious attachment) and cortical volume were found in the cerebellum (right lobule VI and left Crus 2) and neocortex (right medial OrbitoFrontal Cortex, OFC) regions. Cerebellar contribution to the attachment behavioral system reflects the more general cerebellar engagement in the regulation of emotional and social behaviors. Cerebellar properties of timing, prediction, and learning well integrate with OFC processing supporting the regulation of attachment experiences. Cerebellar areas might be rightfully included in the attachment behavioral system.
Oxidative stress promotes liver cancer metastasis via a PKA-activated RNF25/ECAD/YAP circuit
Authors: Huang Zhao , Zhou Li , Duan Jiufei , Qin Siyuan , Wang Yu , Jiang Jingwen , Jin Ping , Li Bowen , Luo Maochao , He Bo , Li Lei , Wu Peijie , Qiao Ling , Chen Haining , Nice Edouard , Wei Yuquan , Huang Canhua 
Published: January 2022

Abstract
Loss of E-cadherin (ECAD), often caused by epigenetic inactivation, is closely associated with tumor metastasis. However, how ECAD is regulated in response to oxidative stress during tumorigenesis is largely unknown. Here we identify RNF25 as a new E3 ligase of ECAD, whose activation by oxidative stress leads to ECAD protein degradation in hepatocellular carcinoma (HCC). Loss of ECAD activates YAP, which in turn promotes the transcription of RNF25, thus forming a positive feedback loop to sustain the ECAD downregulation. YAP activation mitigates oxidative stress in detached HCC cells by upregulating antioxidant genes, protecting detached HCC cells from ferroptosis, resulting in anoikis resistance. Mechanistically, we found that protein kinase A (PKA) senses oxidative stress by redox modification in its β catalytic subunit (PRKACB) at Cys200 and Cys344, which increases its kinase activity towards RNF25 phosphorylation at Ser450, facilitating RNF25-mediated degradation of ECAD. Moreover, RNF25 expression is associated with HCC metastasis and depletion of RNF25 is sufficient to diminish HCC invasion and metastasis in vitro and in vivo. Together, these results identify a dual role of RNF25 as a critical regulator of ECAD protein turnover, promoting both anoikis resistance and metastasis, and PKA is a necessary redox sensor to enable this process. Our study provides mechanistic insight into how tumor cells sense oxidative stress signals to spread while escaping cell death.
Colocolic intussusception in children: A case series and literature review
Authors: Yan Jiayu , Shen Qiulong , Peng Chunhui , Pang Wenbo , Chen Yajun 
Published: January 2022

Abstract
Background
Colocolic intussusceptions is a rare subtype of intussusception mostly caused by juvenile polyps. The treatment of colocolic intussusception caused by other pathologic lead points remains poorly understood.
Methods
A systematic literature review between January 2000 and June 2021 was performed to characterize the comprehensive treatment of colocolic intussusception in children. This report also included 10 patients admitted to our center between 2010 and 2020 not previously reported in the literature.
Results
We identified 27 patients in 20 studies in addition to 10 patients from our center for a total of 37 patients (median age, 4.0 years; 54.1% male). The lead point was identified in 33 patients (33/37, 89.2%). The most common lead point was juvenile polyps (16/33, 48.5%). A therapeutic enema was performed in 12 patients with colocolic intussusception caused by juvenile polyps and was successful in 8 patients (8/12, 66.7%). Colonoscopic polypectomy was subsequently performed in 7 patients and was successful in 6 patients (6/7, 85.7%). The other patient had undergone laparoscopic exploration, and no abnormality was found. Subsequently, the patient underwent open surgery. The patients with colocolic intussusception caused by other pathologic lead points almost underwent surgical treatment (14/17, 82.4%), including 12 open surgeries and 2 laparoscopic surgeries.
Conclusion
A therapeutic enema followed by colonoscopic polypectomy is feasible to treat colocolic intussusception caused by juvenile polyps unless the patient has bowel perforation; however, open surgery is sometimes needed. For patients with colocolic intussusception caused by other pathologic lead points, open surgery may be preferable to laparoscopic surgery.
Novel Data About Unusual Location of Toxoplasma Gondii Within Active Microbial Community in the Anterior Chamber of the Eye of Egyptian Rural Children
Authors: Eleinen Khaled G. Abu , Abdelaal Amany A. , Nadar Ahmed H. , El-Adawy Azza I. , Sayed Ahmed , Diab Aya A. , Samir Omar , Abdel-Hafez Yosra N. , Abdel-Shafi Iman R. , Kotb Ahmed A. , Saif Ahmed T.S. , Youseif Ahmed B. , Alatyar Adham A. , Sheble Mostafa A. , Noman Aser M. , EI-Dardiry Marwa Ahmed 
Published: January 2022

Abstract
In Egypt, many cases of granulomatous anterior uveitis consisting of single or multiple gelatinous nodules were detected in children living in rural areas. These lesions are believed to be waterborne and were previously attributed to flatworms ‘stage, showing some improvement after antiparasitic treatment. In a trial to explore the nature of these ocular lesions among rural Egyptian children, twenty surgically excised ocular lesions were subjected to transmission electron microscopy (TEM) examination. TEM results were combined with previous results of the metagenomic analysis performed for four cases out of the twenty samples, revealing the presence of Toxoplasma gondii (T. gondii), besides, a wide range of microbial communities, including variable species of fungi, bacteria, and archaea. The excised lesions ranged from 1 to 5 mm in size and demonstrated an extensive inflammatory cellular infiltrate. Using TEM, five out of twenty samples revealed active eukaryotic organisms with intact energetic cellular organelles, besides, numerous nuclei encircled within a syncytial layer and enclosed by a hyaline layer rich in mitochondria. Six samples showed inactivity in the cellular and the covering portions, while just inflammatory reaction was seen in the remaining nine samples. Toxoplasma gondii was found free within the distal part of the syncytium while, the proximal part showed the active synthesis of possibly extra polymeric substance, perhaps secreted by the microbial community. In a conclusion, Toxoplasma gondii has been detected among a microbial community in an atypical lesion in the eye. Further studies need to be sustained on genotype characterization, proteomic analysis, besides, the aquatic transmission of these mixed microbial species to the ocular tissues to clarify the reason behind such ocular illness.