bluuebunny/crossref_metadata_2025_split huggingface dataset:
Repetitive mild TBI causes pTau aggregation in nigra without altering preexisting fibril induced Parkinson’s-like pathology burden
Authors: Delic Vedad , Karp Joshua H. , Guzman Maynard , Arismendi Gabriel R. , Stalnaker Katherine J. , Burton Julia A. , Murray Kathleen E. , Stamos Joshua P. , Beck Kevin D. , Sokratian Arpine , West Andrew B. , Citron Bruce A. 
Published: November 2022

AbstractPopulation studies have shown that traumatic brain injury (TBI) is associated with an increased risk for Parkinson’s disease (PD) and among U.S. Veterans with a history of TBI this risk is 56% higher. The most common type of TBI is mild (mTBI) and often occurs repeatedly among athletes, military personnel, and victims of domestic violence. PD is classically characterized by deficits in fine motor movement control resulting from progressive neurodegeneration of dopaminergic neurons in the substantia nigra pars compacta (SNpc) midbrain region. This neurodegeneration is preceded by the predictable spread of characteristic alpha synuclein (αSyn) protein inclusions. Whether repetitive mTBI (r-mTBI) can nucleate PD pathology or accelerate prodromal PD pathology remains unknown. To answer this question, an injury device was constructed to deliver a surgery-free r-mTBI to rats and human-like PD pathology was induced by intracranial injection of recombinant αSyn preformed fibrils. At the 3-month endpoint, the r-mTBI caused encephalomalacia throughout the brain reminiscent of neuroimaging findings in patients with a history of mTBI, accompanied by astrocyte expansion and microglial activation. The pathology associated most closely with PD, which includes dopaminergic neurodegeneration in the SNpc and Lewy body-like αSyn inclusion burden in the surviving neurons, was not produced de novo by r-mTBI nor was the fibril induced preexisting pathology accelerated. r-mTBI did however cause aggregation of phosphorylated Tau (pTau) protein in nigra of rats with and without preexisting PD-like pathology. pTau aggregation was also found to colocalize with PFF induced αSyn pathology without r-mTBI. These findings suggest that r-mTBI induced pTau aggregate deposition in dopaminergic neurons may create an environment conducive to αSyn pathology nucleation and may add to preexisting proteinaceous aggregate burden.
Psychometric evaluation of the Estonian version of the Semi-structured Interview for Personality Functioning DSM-5 (STiP-5.1)
Authors: Oitsalu Maarja-Liisa , Kreegipuu Maie , Hutsebaut Joost 
Published: December 2022

Abstract
Background
The DSM-5 Alternative Model for Personality Disorders introduced a dimensional perspective on personality disorders. The model assesses functioning in four domains: Identity, Self-Direction, Empathy, and Intimacy. This study evaluates the psychometric properties of the Semi-Structured Interview for Personality Functioning DSM-5 (STiP-5.1) in Estonian.

Method
The sample consists of 131 participants: 58 from the general population and 73 from a mixed clinical sample that is further divided into a mood and anxiety disorder sample and personality disorder sample. All participants completed the STiP-5.1 interview and the Level of Personality Functioning Scale–Brief Form (LPFS-BF 2.0).

Results
The Estonian STiP-5.1 interview has good internal consistency (McDonald's ω between .94–.98) and high convergent validity (correlations with LPFS-BF 2.0 above .7). Interview scores successfully differentiated the general population from the mixed clinical sample (Cohen’s d = 2.68), as well as patients with personality disorder from those without (Cohen’s d = 1.76). The LPFS-BF 2.0 total score differentiates the general population sample from the mixed clinical sample (Cohen’s d = 1.99) but not the personality disorder sample from other clinical sample participants.

Conclusions
The properties of the Estonian STiP-5.1 replicate those of other languages, and empirically support a unified personality functioning dimension that can be meaningfully thought of as reflecting impairments in self and interpersonal functioning. Findings of this study will be discussed in the light of the ongoing debate on the dimensionality of personality pathology and the use of self-report versus interview measures for assessing personality pathology.
First report of chemical composition and cytotoxicity evaluation of Foraminispora rugosa basidiomata from Brazil
Authors: Garcia V. O. , Fronza M. , Von Borowski R. , Alves-Silva G. , Zimmer A. R. , Ruaro T. , Gnoatto S. C. B. , Dallegrave A. , Silveira R. M. B. 
Published: November 2022

Abstract
Background
Foraminispora rugosa is a species reported from Brazil, Venezuela, French Guiana, Costa Rica and Cuba. It is a basidiomycete in the Ganodermataceae family. In this study, both chemical composition and cytotoxicity of the ethanolic extract of F. rugosa were investigated for the first time.

Results
Phylogenetic analysis confirmed the identification of the specimens, and the results of cytotoxicity assays showed that at concentrations of 7.8–500.0 µg/mL the ethanolic extract displayed weak cytotoxicity against the tested cell lines. Five oxylipins were identified by ultra high performance liquid chromatography coupled with quadrupole time-of-flight and mass spectrometry (UHPLC-QTOF–MS).

Conclusions
This study provides new insights into the current knowledge of bioactive compounds produced by macrofungi, and provides data for future biological assays with relative selectivity and safety.
Ionospheric responses on the 21 August 2017 solar eclipse by using three-dimensional GNSS tomography
Authors: Chen C. H. , Lin Charles C. H. , Lee C. J. , Liu J. Y. , Saito A. 
Published: November 2022

AbstractIn this study, we have investigated the ionospheric responses on the August 2017 solar eclipse event by using a three-dimensional tomography algorithm with the ground-based GNSS (Global Navigation Satellite System) total electron content observations around Northern America. This three-dimensional ionospheric electron density structure from the tomography can provide us more information regarding the density variations and propagations of disturbances. Results show that the ionospheric electron density depletion triggered by the solar eclipse started from the higher ionosphere and then extended to lower altitudes. The maximum electron density depletion is around 40% compared with the previous day of solar eclipse. After around 30 min of the totality, the electron density continuously returned to the normal level. We further conduct a procedure of Fourier analyses to derive the vertical phase and group velocities of the electron density propagations. Results show that the opposite directions of the vertical phase and group velocities around 220–240 km altitude imply the energy/oscillation source by the solar eclipse.
Graphical Abstract
Three-dimensional shape distribution of lunar regolith particles collected by the Apollo and Luna programs
Authors: Tsuchiyama Akira , Sakurama Takashi , Nakano Tsukasa , Uesugi Kentaro , Ohtake Makiko , Matsushima Takashi , Terakado Kazuo , Galimov Erik M. 
Published: November 2022

AbstractThe shapes of regolith particles on airless bodies, such as the Moon and asteroids, are important to understand their formation and evolution on surfaces. Limited studies have shown that the three-dimensional (3D) shapes of lunar regolith particles are, on average, more equant (spherical) than those of asteroid Itokawa or fragments by impact experiments. Therefore, more studies are required to determine whether such a feature is common. Accordingly, we performed X-ray microtomography imaging of lunar regolith particles collected by the Apollo program by NASA and the Luna program by the Soviet Union to obtain their 3D shapes. The ten samples (65 to 1108 particles/sample) examined had varieties of sampling sites (maria and highlands), reflecting the difference in materials (basalts and anorthosites, respectively, in general), regolith maturities, particle size ranges (< 74 to 450 µm), and petrographic textures (monomineralic, polymineralic, and agglutinate). The 3D particle shape distributions regarding three-axial length ratios (L:I:S, where L, I, and S are the longest, intermediate, and shortest lengths, respectively) showed that the average three-axial ratios were almost similar among the samples, irrespective of the sampling sites, maturities, and the size ranges [S/I = 0.770(8), I/L = 0.758(10), and S/L = 0.581(11) for whole samples]. The 3D shapes of lunar particles were more equant (spherical) than those of the particles collected from asteroid Itokawa and fragments by hypervelocity impact experiments which had the average ratios similar to the 2D silver ratio (S/I = I/L = 0.707 and S/L = 0.500). These findings showed that the balance between impact fragmentation and mechanical abrasion controls the 3D shapes of lunar particles because impact and particle motion on the Moon’s surface occur for a longer duration; however, impact fragmentation on this small asteroid surface primarily controls those of Itokawa particles. We also found shape dependence on petrographic textures of the lunar particles, and this could be explained by the strength of the materials against abrasion. The results obtained in this study will be the basic data to be compared with upcoming new results, such as particles collected from asteroid Ryugu, possibly from asteroid Bennu and Martian moons.
Graphical Abstract
Diagnostic performance and prognostic value of preoperative 18F-FDG PET/CT in renal cell carcinoma patients with venous tumor thrombus
Authors: Chen Silu , Zhao Yanyan , Tang Qi , Wu Caixia , Wang Aixiang , Ma Linlin , Zhang Xi , Chen Jinzhi , Gao Yuan , Liao Xuhe , Feng Ninghan , Fan Yan , Zhang Jianhua , Li Xuesong , Liu Meng 
Published: November 2022

AbstractBackgroundTo observe the diagnostic efficacy of preoperative fluorine-18 fluorodeoxyglucose (18F-FDG) positron emission tomography/computed tomography (PET/CT) upon venous tumor thrombus (VTT) in patients with renal cell carcinoma (RCC), and investigate the prognostic value of imaging parameters integrated with clinicopathological characteristics in patients with VTT after nephrectomy with tumor thrombectomy.MethodsPatients with newly diagnosed RCC who underwent18F-FDG PET/CT were reviewed retrospectively. The diagnostic efficacy of18F-FDG PET/CT in VTT was analyzed. Logistic regression analysis was carried out to identify the clinical variables and PET/CT variables (including maximum standardized uptake value (SUVmax) of primary tumor, VTT SUVmax and primary tumor size) for differentiating early VTT (Mayo 0-II) from advanced VTT (Mayo III-IV). Cox proportional hazard analyses were used to evaluate clinicopathological factors and PET/CT factors (including distant metastasis, primary tumor SUVmax, VTT SUVmax and primary tumor size) for disease-free survival (DFS) in patients with VTT after operation.ResultsA total of 174 eligible patients were included in this study, including 114 men (65.5%) and 60 women (34.5%), with a median age of 58 years (range, 16–81 years). The distribution of pathological tumor stage (T stage) was 56 (T1), 17 (T2), 95 (T3), and 6 cases (T4), respectively. According to WHO/ISUP grade, except for 4 cases of chromophobe cell RCC, there were 14 patients (8.0%) of grade 1, 59 patients (33.9%) of grade 2, 74 patients (42.5%) of grade 3 and 23 patients (13.2%) of grade 4. The median maximum diameter of the primary tumor on PET/CT was 7.3 cm (5.0–9.5 cm). The distal metastasis was observed in 46 patients (26.4%). Sixty-one cases (35.1%) were confirmed with VTT by pathology. The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of18F-FDG PET/CT imaging were 96.7, 99.1, 98.3, 98.3, and 98.2%, in detecting VTT, respectively, and 70.0, 100.0, 94.9, 100.0, and 94.2%, in evaluating the level of VTT, respectively. Elevated VTT SUVmax (≥5.20) could significantly distinguish the early VTT group and advanced VTT group (P = 0.010). In the prognosis analysis, elevated VTT SUVmax (≥4.30) (P = 0.018, HR 3.123, 95% CI 1.212–8.044) and distant metastasis (P = 0.013, HR 3.344, 95% CI 1.293–8.649) were significantly independent predictors for DFS.ConclusionPreoperative18F-FDG PET/CT has a high diagnostic efficacy in detecting VTT and evaluating its level in RCC patients. Those patients with elevated VTT SUVmax should be carefully monitored to detect the possibility of disease progression after operation.
Extracellular signals regulate the biogenesis of extracellular vesicles
Authors: Jin Yong , Ma Lele , Zhang Wanying , Yang Wen , Feng Qiyu , Wang Hongyang 
Published: November 2022

AbstractExtracellular vesicles (EVs) are naturally released membrane vesicles that act as carriers of proteins and RNAs for intercellular communication. With various biomolecules and specific ligands, EV has represented a novel form of information transfer, which possesses extremely outstanding efficiency and specificity compared to the classical signal transduction. In addition, EV has extended the concept of signal transduction to intercellular aspect by working as the collection of extracellular information. Therefore, the functions of EVs have been extensively characterized and EVs exhibit an exciting prospect for clinical applications. However, the biogenesis of EVs and, in particular, the regulation of this process by extracellular signals, which are essential to conduct further studies and support optimal utility, remain unclear. Here, we review the current understanding of the biogenesis of EVs, focus on the regulation of this process by extracellular signals and discuss their therapeutic value.
Hypercalcemia affected in metastatic breast cancer patients without bone metastasis: report of three cases
Authors: Hashizume Sakina , Shimoda Masafumi , Yoshinami Tetsuhiro , Abe Kaori , Masunaga Nanae , Sota Yoshiaki , Miyake Tomohiro , Tanei Tomonori , Shimazu Kenzo 
Published: November 2022

Abstract
Background
Since humoral hypercalcemia of malignancy (HHM) in breast cancer patients without bone metastasis is rare, the clinical features of this condition are not fully understood.

Case presentation
During the recent 12 years, 3602 patients were diagnosed with breast cancer in our institution, and only three patients developed HHM without bone metastasis. They were all recurrent breast cancer patients with visceral metastases including the lung and the liver. It took no more than 2 months since symptomatic onset to hospitalization because of hypercalcemia. The maximum serum calcium concentrations were 15.0 mg/dL or higher. All patients had symptoms related to hypercalcemia. Treatment of hypercalcemia including hydration, calcitonin, bisphosphonate, and diuretics was initially effective in the three patients. However, two of three cases were eventually fatal because of unsuccessful treatment of breast cancer.

Conclusions
The common features of HHM without bone metastasis in breast cancer patients include acute onset, severe symptomatic hypercalcemia, and presence of visceral metastasis. Treatment of hypercalcemia decreased serum calcium level in a short period, while successful treatment of breast cancer was essential for a long-term management of HHM. This report provides a consideration to help elucidate the pathophysiology and medical care of breast cancer patients with HHM without bone metastasis.
Expanding known viral diversity in plants: virome of 161 species alongside an ancient canal
Authors: Yang Shixing , Mao Qingqing , Wang Yan , He Jingxian , Yang Jie , Chen Xu , Xiao Yuqing , He Yumin , Zhao Min , Lu Juan , Yang Zijun , Dai Ziyuan , Liu Qi , Yao Yuxin , Lu Xiang , Li Hong , Zhou Rui , Zeng Jian , Li Wang , Zhou Chenglin , Wang Xiaochun , Shen Quan , Xu Hui , Deng Xutao , Delwart Eric , Shan Tongling , Zhang Wen 
Published: November 2022

Abstract
Background
Since viral metagenomic approach was applied to discover plant viruses for the first time in 2006, many plant viruses had been identified from cultivated and non-cultivated plants. These previous researches exposed that the viral communities (virome) of plants have still largely uncharacterized. Here, we investigated the virome in 161 species belonging to 38 plant orders found in a riverside ecosystem.

Results
We identified 245 distinct plant-associated virus genomes (88 DNA and 157 RNA viruses) belonging to 27 known viral families, orders, or unclassified virus groups. Some viral genomes were sufficiently divergent to comprise new species, genera, families, or even orders. Some groups of viruses were detected that currently are only known to infect organisms other than plants. It indicates a wider host range for members of these clades than previously recognized theoretically. We cannot rule out that some viruses could be from plant contaminating organisms, although some methods were taken to get rid of them as much as possible. The same viral species could be found in different plants and co-infections were common.

Conclusions
Our data describe a complex viral community within a single plant ecosystem and expand our understanding of plant-associated viral diversity and their possible host ranges.
A multi-country, prospective cohort study to evaluate the economic implications of relapse among children recovered from severe acute malnutrition: a study protocol
Authors: Puett Chloe , King Sarah , Stobaugh Heather 
Published: November 2022

Abstract
Background
Community-based management of acute malnutrition (CMAM) is an effective intervention at recovering children from severe acute malnutrition (SAM) and preventing mortality. However, there is growing evidence that for many children recovery is not sustained post-discharge. This study will assess the economic implications of relapse by calculating the average cost of treating a case of SAM that relapses after initial CMAM treatment compared to the cost of a case that remains recovered for 6 months post-discharge.

Methods
This protocol outlines the methods for a cost-efficiency analysis to assess cost per episode of treatment for acute malnutrition for children enrolled in CMAM programs for initial SAM treatment in Mali, Somalia and South Sudan. Cost data will be collected and analyzed on a monthly basis for each CMAM service component (outpatient treatment program for SAM, supplementary feeding program for moderate acute malnutrition, and inpatient stabilization care for SAM with medical complications). Financial data will be extracted from expenditure records from institutional accounting systems where possible. Where these are not present, cost data will be collected via interview and review of financial documents. Staff time allocation interviews will be conducted. This data will be applied to quantify personnel costs, to apportion costs that are shared between programs and to exclude staff time spent on research activities.

Discussion
This study will provide the first estimates to address the limited evidence on the economic implications of SAM relapse in CMAM programs. Data from this economic analysis will help raise awareness and provide actionable data for the global nutrition community to address the financial burden of relapse. Estimating the cost of relapse in three countries representing different geographic and operational contexts will help in generalizing these results.

Trial registration
Registration # IORG0007116, Date of registration: 06/09/2020. This study is not registered as a clinical trial as it is observational research and does not include an intervention. The study has received the required ethical approvals as outlined in the declarations.
Modified exosomal SIRPα variants alleviate white matter injury after intracerebral hemorrhage via microglia/macrophages
Authors: Gao Xinjie , Yang Heng , Xiao Weiping , Su Jiabin , Zhang Yuwen , Wang He , Ni Wei , Gu Yuxiang 
Published: November 2022

Abstract

Background
Despite limited efficiency, modulation of microglia/macrophages has shown to attenuate neuroinflammation after intracerebral hemorrhage (ICH). In this context, we evaluated the efficacy of modified exosomal signal regulatory protein α (SIRPα) variants (SIRPα-v Exos) in microglia/macrophages and neuroinflammation-associated white matter injury after ICH.

Methods
SIRPα-v Exos were engineered to block CD47-SIRPα interactions. After obtaining SIRPα-v Exos from lentivirus-infected mesenchymal stem cells, C57BL/6 mice suffering from ICH underwent consecutive intravenous injections of SIRPα-v Exos (6 mg/kg) for 14 days. Afterwards, the volume of hematoma and neurological dysfunctions were assessed in mice continuously until 35 days after ICH. In addition, demyelination, electrophysiology and neuroinflammation were evaluated. Furthermore, the mechanisms of microglial regulation by SIRPα-v Exos were investigated in vitro under coculture conditions.

Results
The results demonstrated that the clearance of hematoma in mice suffering from ICH was accelerated after SIRPα-v Exo treatment. SIRPα-v Exos improved long-term neurological dysfunction by ameliorating white matter injury. In addition, SIRPα-v Exos recruited regulatory T cells (Tregs) to promote M2 polarization of microglia/macrophages in the peri-hematoma tissue. In vitro experiments further showed that SIRPα-v Exos regulated primary microglia in a direct and indirect manner in synergy with Tregs.

Conclusion
Our studies revealed that SIRPα-v Exos could accelerate the clearance of hematoma and ameliorate secondary white matter injury after ICH through regulation of microglia/macrophages. SIRPα-v Exos may become a promising treatment for ICH in clinical practice.

Graphical Abstract
Recent biomedical advancements in graphene oxide- and reduced graphene oxide-based nanocomposite nanocarriers
Authors: Bellier Naline , Baipaywad Phornsawat , Ryu Naeun , Lee Jae Young , Park Hansoo 
Published: November 2022

Abstract
Recently, nanocarriers, including micelles, polymers, carbon-based materials, liposomes, and other substances, have been developed for efficient delivery of drugs, nucleotides, and biomolecules. This review focuses on graphene oxide (GO) and reduced graphene oxide (rGO) as active components in nanocarriers, because their chemical structures and easy functionalization can be valuable assets for in vitro and in vivo delivery. Herein, we describe the preparation, structure, and functionalization of GO and rGO. Additionally, their important properties to function as nanocarriers are presented, including their molecular interactions with various compounds, near-infrared light adsorption, and biocompatibility. Subsequently, their mechanisms and the most appealing examples of their delivery applications are summarized. Overall, GO- and rGO-based nanocomposites show great promise as multipurpose nanocarriers owing to their various potential applications in drug and gene delivery, phototherapy, bioimaging, biosensing, tissue engineering, and as antibacterial agents.