bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Vitro Biological Control of Fungus Botrydiplodia sp. Using Plant-based Pesticide
Authors: Herliyana E N , Istikorini Y , Septyan A R 
Published: January 2022

Abstract

Botryodiplodia sp. is a pathogenic fungus with a wide range of hosts, including agricultural and forestry crops. Botryodiplodia sp. attacks have been reported to cause symptoms of dry rot, branch rot, fruit rot, and shoot death resulting in decreased productivity. Efforts to control diseases caused by the fungus Botryodiplodia sp. are needed to avoid further infection in healthy plants. Control using chemical pesticides will cause several negative problems for the environment and human health. Alternative control of Botryodiplodia sp. is using biopesticide. This study aimed to analyze the ability of plant extract-based pesticides to inhibit the growth of Botryodiplodia sp. in vitro. The method used was the food poisoning method on PDA and PDB with concentrations of biopesticide 0%, 0.25%, 0.5%, 1%, 4%, 7%, and 10%. The result of this research is the concentration of 0.5% is sufficient to inhibit the growth of Botrydiplodia sp by 11.73% in PDA and 21.96% in PDB. Biopesticides are suspected to have fungistatic characteristics so that they only inhibit but do not stop fungal growth.
Effect of Pyrolysis Methods on Characteristics of Biochar from Young Coconut Waste as Ameliorant
Authors: Maulana A , Herviyanti  , Prasetyo T B , Harianti M , Lita A L 
Published: January 2022

Abstract
Biochar is produced from the pyrolysis of organic matter (waste and biomass of agriculture) e.g young coconut waste (YCW). However, there is little information describing methods for producing biochar as an ameliorant for agriculture. Therefore, research is needed to determine the biochar characteristics of the Kon-Tiki, Drum, and Soil-Pit methods. This study was conducted using a Completely Randomized Design with three replications. The results of biochar production with methods significant on temperature (682°C), moisture (81.27 %), dry weight (2.09 kg) and yield ratio (20.87%) of biochar, and characteristics of biochar from YCW on pH (10.82 unit), liming potential (5.50% CaCO3), proximate analysis (moisture of 38.80%; volatile matter of 62.73%; ash of 19.50% and fixed carbon of 43.22%), CEC (457.13 mmol kg−1), cation base (K of 356.14 and Na-exch of 131.28 mmol kg−1), Cárgame (0.35 g kg−1), and C Organic (14.27 g kg−1), with the recommended method of YCW biochar production using the Kon-Tiki method.
The impact of revegetation activities in various post-mining lands in Indonesia (study of literature)
Authors: Lestari K G , Budi S W , Suryaningtyas D T 
Published: January 2022

AbstractMany revegetation activities have been carried out on post-mining land in Indonesia and have produced various positive impacts on the land. This raises a question that what changes have occurred in various post-mining areas that have been revegetated in Indonesia. The method used a literature review from 37 sources (Journals and research report) related to the impact of revegetation activities in various post-mining lands in Indonesia. The results of this literature review conclude that revegetation in Indonesia greatly affects and has changed 3 main aspects of a land, namely (1) soil conditions both chemically, physically and biologically, (2) improvement of nutrient cycles in revegetation areas and (3) improvement of hydrological cycles and microbial and animal habitats. Suggestions that revegetation activities continue to be carried out and their success monitored and revegetation cooperation with the community will be more profitable.
The infiltration capacity and rate at the grass, building yard and green open space areas of Universitas Gadjah Mada campus
Authors: Wikantyasa B , Kusumandari A 
Published: January 2022

Abstract
Land use and soil greatly determine the rate of infiltration. There are three land uses according to vegetation cover and buildings at the Universitas Gadjah Mada (UGM) campus, namely grassland, building yard and green open space area. The purpose of this study is to determine the capacity and rate of infiltration at the UGM campus area and to design the proposed land use that increases infiltration. This research uses a double-ring infiltrometer, soil samples consist of disturbed and undisturbed. Analysis of infiltration capacity using Horton’s formula, then we analysed the physical and chemical properties of the soil at the laboratory. Based on the results of the study, it concludes that the infiltration capacity of grassland is 12.52 mm/hour, the building yard is 10.89 mm/hour, and the green open space area is 14.02 mm/hour. The infiltration rate of the three land uses is slightly slow. The soil texture is mostly silty clay, crumb soil structure and organic matter content ranges from 1-2.1%. The recommended land use plan for the area of UGM is planting more trees.
Development of frequency comb generation by spectral broadening of periodic optical pulses in semiconductor laser amplifiers
Authors: Sharma Vishal , Singh Surinder , Lovkesh  
Published: January 2022

Abstract
This paper reports an approach for generating an ultra-flat and ultra-wide optical frequency comb by exploiting gain modulation, experienced by a periodic third ordered Gaussian-shaped optical pulse propagating through the semiconductor optical amplifier (SOA). The gain experienced by optical signal propagating inside SOA is independent of its polarization state and phase, makes the technique more favorable and stable to the optical frequency comb generation. This paper reports a 94-line optical frequency comb with 5 dB maximum power deviation and a 190-line optical frequency comb with 10 dB maximum power deviation.
Injection Guidelines for Treating Midface Volume Deficiency With Hyaluronic Acid Fillers: The ATP Approach (Anatomy, Techniques, Products)
Authors: Trévidic Patrick , Kaufman-Janette Joely , Weinkle Susan , Wu Raymond , Dhillon Benji , Antunes Stéphanie , Macé Emilie , Maffert Pauline 
Published: January 2022

AbstractMidface rejuvenation is among the most valuable indications of hyaluronic acid dermal fillers, because malar projection and full upper cheeks significantly contribute to a youthful appearance. Hyaluronic acid fillers have evolved over the past 2 decades to meet specific clinical needs such as strong projection capacity and adaptability to facial dynamism. As a result, they now represent the treatment of choice for midface rejuvenation throughout age ranges by offering the potential for noninvasive treatment, immediate results, and minimal downtime. Because the 5-layered structure of the midface plays a central role in the human face, injecting the midface area may also indirectly improve other aesthetic concerns such as infraorbital hollowing and nasolabial folds. Nonetheless, midface rejuvenation requires a tailored treatment approach and a thorough knowledge of anatomy to minimize procedural risks and achieve natural-looking results. This article provides an extensive anatomical description of the midface and of the usual course and depth of vascular structures circulating nearby to delineate a treatment area and minimize procedural risks. Furthermore, considering the differential mobility and mechanical constraints of each layer of the midface, a multilayer treatment algorithm is proposed for adapting the treatment strategy to patient specificities (including age, gender, skin type, and morphology). Emphasis is also placed on desirable filler properties to create deep structural support on the one hand and accompany facial movement on the other hand.
Distribution of flagellin CD2-1, flg22, and flgII-28 recognition systems in plant species and regulation of plant immune responses through these recognition systems
Authors: Murakami Takahiko , Katsuragi Yuya , Hirai Hiroyuki , Wataya Koki , Kondo Machiko , Che Fang-Sik 
Published: January 2022

ABSTRACTThe first layer of active plant immunity relies upon the recognition of pathogen-associated molecular patterns (PAMPs), and the induction of PTI. Flagellin is the major protein component of the bacterial flagellum. Flagellin-derived peptide fragments such as CD2-1, flg22, and flgII-28 function as PAMPs in most higher plants. To determine the distribution of CD2-1, flg22, and flgII-28 recognition systems within plant species, the inducibility of PTI by CD2-1, flg22, and flgII-28 in 8 plant species, including monocotyledonous and dicotyledonous plants, was investigated. CD2-1 caused PTI responses in Oryza sativa, Brachypodium distachyon, and Asparagus persicus; flg22 caused PTI responses in Phyllostachys nigra, A. persicus, Arabidopsis thaliana, Nicotiana tabacum, Solanum lycopersicum, and Lotus japonicus; and flgII-28 caused PTI responses only in S. lycopersicum. Furthermore, quantitative analysis of FLS2 receptor revealed that the responsiveness of flg22 in plants was dependent on the expression level of the receptor.
Factors influencing quality of lymphadenectomy in minimally invasive esophagectomy: a US-based analysis
Authors: Odetoyinbo Kolade , Bachman Katelynn , Worrell Stephanie , Gray Kelsey , Linden Philip , Towe Christopher 
Published: January 2022

Summary
NCCN guidelines suggest that at least 15 lymph nodes (LN) should be evaluated at the time of esophagectomy to consider the lymphadenectomy ‘adequate’. Despite these guidelines, this may not always be achieved in practice. The purpose of this study was to determine factors associated with adequate lymphadenectomy among patients receiving minimally invasive esophagectomy (MIE). Patients receiving MIE in the National Cancer Database from 2010 to 2016 were identified. Patients with metastatic disease were excluded. The primary endpoint was adequate lymphadenectomy, defined as &gt;15 or greater LN evaluated. Factors associated with adequate lymphadenectomy and overall survival were evaluated in univariable and multivariable analyses. Categorical variables were assessed using chi-squared, and continuous variables were assessed with rank-sum test. Survival was evaluated using the Kaplan–Meier method. A total of 6,539 patients underwent MIE between 2010 and 2016 (5,024 thoracoscopic–laparoscopic MIE and 1,515 robotic-assisted MIE). A total of 3,527 patients (53.9%) received adequate lymphadenectomy. Receiving MIE at an academic center (odds ratio [OR] 1.37, 95% confidence interval [CI] 1.15–1.63, P &lt; 0.001), institutional volume of MIE (OR 1.01, 95% CI 1.008–1.011, P &lt; 0.001), and presence of clinical nodal disease (OR 1.17, 95%CI 1.02–1.33, P = 0.025) were associated with adequate lymphadenectomy. Patients with &gt;15 LN removed had increased overall survival (46.6 vs. 41.5 months, P &lt; 0.001). Adequate lymphadenectomy (hazard ratio [HR] 0.77, 95%CI 0.71–0.85, P &lt; 0.001), receiving surgery at an academic center (HR 0.87, 95%CI 0.78–0.96, P = 0.007) and private insurance status (HR 0.88, 95%CI 0.81–0.98, P = 0.02) were independently associated with improved survival. Nearly half of patients receiving MIE do not receive adequate lymphadenectomy as defined by NCCN guidelines. Receiving MIE at an academic center with high procedural volume and the presence of nodal disease were independently associated with adequate lymphadenectomy. Adequate lymphadenectomy was associated with improved survival. These findings suggest that providers performing esophagectomy should follow guideline-based recommendations for lymphadenectomy.
Right atrium angiosarcoma with feeding vessels from right coronary artery: a case report
Authors: Yeh Jian-Kuan , Tsai Yi-Shan , Chen Ya-Ping , Roan Jun-Neng , Chang Hsien-Yuan 
Published: January 2022

Abstract

Background
Primary cardiac angiosarcoma is a rare primary cardiac malignancy. Biopsy of such vascular-rich tumours may result in serious complications.

Case summary
This is a case of a 43-year-old woman who presented with syncope. According to clinical history, she initially had massive pericardial effusion, with an uncertain aetiology. Multimodality imaging at our hospital revealed a cauliflower-like tumour in the right atrium. Coronary angiography results confirmed multiple feeding vessels from the right coronary artery to the tumour. Thoracoscopic biopsy resulted in a massive bleeding requiring haemostasis via thoracotomy. Histopathological examination of the specimen showed an angiosarcoma with atypical cells and spindle cells in a myxomatous background. Treatment with systemic targeted therapy and chemotherapy was initiated, and the patient is still under active treatment.

Discussion
Cardiac angiosarcomas most commonly arise from the right atrium and may be hard to detect with transthoracic echocardiography. Biopsy of primary cardiac angiosarcomas requires careful planning because they are highly vascularized. Currently, no guidelines regarding the treatment of such tumours exist, and a multidisciplinary treatment is needed.
Functional Connectivity of the Supplementary and Presupplementary Motor Areas in Postoperative Transition Between Stages of Frailty
Authors: Lammers-Lietz Florian , Zacharias Norman , Mörgeli Rudolf , Spies Claudia D , Winterer Georg 
Published: January 2022

Abstract

Background
Frailty is a multietiological geriatric syndrome of run-down physical reserves with high vulnerability to stressors. Transitions between physical robustness and frailty often occur in the context of medical interventions. Studies suggest that neurological disorders contribute to faster progression of frailty. In a previous cross-sectional study we found altered functional connectivity of supplementary motor area (SMA) in (pre)frail compared to robust patients. We analyzed functional connectivity of the SMA and presupplementary motor area (pre-SMA) in patients with postoperative transitions between physical robustness and stages of frailty.

Methods
We investigated 120 cognitively healthy patients (49.2% robust, 47.5% prefrail, 3.3% frail, 37.5% female, median age 71 [65–87] years) undergoing elective surgery from the BioCog project, a multicentric prospective cohort study on postoperative delirium and cognitive dysfunction. Assessments took place 14 days before and 3 months after surgery, comprising assessments of a modified frailty phenotype according to Fried and resting-state functional magnetic resonance imaging at 3 T. The associations between functional connectivity of the SMA and pre-SMA networks, preoperative frailty stages, and postoperative transitions were examined using mixed linear effects models.

Results
Nineteen patients showed physical improvement after surgery, 24 patients progressed to (pre)frailty and in 77 patients no transition was observed. At follow-up, 57 (47.5%) patients were robust, 52 (43.3%) prefrail, and 11 (9.2%) frail. Lower functional connectivity in the pre-SMA network was associated with more unfavorable postoperative transition types. An exploratory analysis suggested that the association was restricted to patients who were prefrail at baseline. There was no association of transition type with SMA functional connectivity in the primary analysis. In an exploratory analysis, transition from prefrailty to robustness was associated with higher functional connectivity and progression in robust patients was associated with higher SMA network segregation.

Conclusions
Our findings implicate that dysfunctions of cortical networks involved in higher cognitive control of motion are associated with postoperative transitions between frailty stages. The pre-SMA may be a target for neurofeedback or brain stimulation in approaches to prevent frailty.
Clinical Trials Registration Number: NCT02265263
Extent of wild–to–crop interspecific introgression in grapevine (Vitis vinifera) as a consequence of resistance breeding and implications for the crop species definition
Authors: Foria Serena , Magris Gabriele , Jurman Irena , Schwope Rachel , De Candido Massimo , De Luca Elisa , Ivanišević Dragoslav , Morgante Michele , Di Gaspero Gabriele 
Published: January 2022

AbstractOver the past two centuries, introgression through repeated backcrossing has introduced disease resistance from wild grape species into the domesticated lineage Vitis vinifera subsp. sativa. Introgression lines are being cultivated over increasing vineyard surface areas, as their wines now rival in quality those obtained from preexisting varieties. There is, however, a lot of debate about whether and how wine laws defining commercial product categories, which are based on the classification of V. vinifera and interspecific hybrid grapes, should be revised to accommodate novel varieties that do not fit either category. Here, we developed a method of multilocus genotype analysis using short–read resequencing to identify haplotypic blocks of wild ancestry in introgression lines and quantify the physical length of chromosome segments free–of–introgression or with monoallelic and biallelic introgression. We used this genomic data to characterize species, hybrids and introgression lines and show that newly released resistant varieties contain 76.5–94.8% of V. vinifera DNA. We found that varietal wine ratings are not always commensurate with the percentage of V. vinifera ancestry and linkage drag of wild alleles around known resistance genes persists over at least 7.1–11.5 Mb, slowing down the recovery of the recurrent parental genome. This method also allowed us to identify the donor species of resistance haplotypes, define the ancestry of wild genetic background in introgression lines with complex pedigrees, validate the ancestry of the historic varieties Concord and Norton, and unravel sample curation errors in public databases.
Characterization of Terpene synthase variation in flowers of wild Aquilegia species from Northeastern Asia
Authors: Yang Song , Wang Ning , Kimani Shadrack , Li Yueqing , Bao Tingting , Ning Guogui , Li Linfeng , Liu Bao , Wang Li , Gao Xiang 
Published: January 2022

Abstract
There are several causes for the great diversity in floral terpenes. The terpene products are determined by the catalytic fidelity, efficiency and plasticity of the active sites of terpene synthases (TPSs). However, the molecular mechanism of TPS in catalyzing terpene biosynthesis and its evolutionary fate in wild plant species remain largely unknown. In this study, the functionality of terpene synthases and their natural variants were assessed in two Northeastern Asia endemic columbine species and their natural hybrid. Synoptically, TPS7, TPS8, and TPS9 were highly expressed in these Aquilegia species from the Zuojia population. The in vitro and in vivo enzymatic assays revealed that TPS7 and TPS8 mainly produced (+)-limonene and β-sesquiphellandrene, respectively, whereas TPS9 produced pinene, similar to the major components released from Aquilegia flowers. Multiple sequence alignment of Aquilegia TPS7 and TPS8 in the Zuojia population revealed amino acid polymorphisms. Domain swapping and amino acid substitution assays demonstrated that 413A, 503I and 529D had impacts on TPS7 catalytic activity, whereas 420G, 538F and 545 L affected the ratio of β-sesquiphellandrene to β-bisabolene in TPS8. Moreover, these key polymorphic amino acid residues were found in Aquilegia species from the Changbai Mountain population. Interestingly, amino acid polymorphisms in TPSs were present in individuals with low expression levels, and nonsynonymous mutations could impact the catalytic activity or product specificity of these genes. The results of this study will shed new light on the function and evolution of TPS genes in wild plant species and are beneficial to the modification of plant fragrances.
Quantitative trait loci mapping for free amino acid content using an albino population and SNP markers provides insight into the genetic improvement of tea plants
Authors: Huang Rong , Wang Jun-Ya , Yao Ming-Zhe , Ma Chun-Lei , Chen Liang 
Published: January 2022

Abstract
Free amino acids are one of the main chemical components in tea, and they contribute to the pleasant flavor, function, and quality of tea, notably the level of theanine. Here, a high-density genetic map was constructed to characterize quantitative trait loci (QTLs) for free amino acid content. A total of 2688 polymorphic SNP markers were obtained using genotyping-by-sequencing (GBS) based on 198 individuals derived from a pseudotestcross population of “Longjing 43” × “Baijiguan”, which are elite and albino tea cultivars, respectively. The 1846.32 cM high-density map with an average interval of 0.69 cM was successfully divided into 15 linkage groups (LGs) ranging from 93.41 cM to 171.28 cM. Furthermore, a total of 4 QTLs related to free amino acid content (theanine, glutamate, glutamine, aspartic acid and arginine) identified over two years were mapped to LG03, LG06, LG11 and LG14. The phenotypic variation explained by these QTLs ranged from 11.8% to 23.7%, with an LOD score from 3.56 to 7.7. Furthermore, several important amino acid metabolic pathways were enriched based on the upregulated differentially expressed genes (DEGs) among the offspring. These results will be essential for fine mapping genes involved in amino acid pathways and diversity, thereby providing a promising avenue for the genetic improvement of tea plants.
Exploiting sterility and fertility variation in cytoplasmic male sterile vegetable crops
Authors: Xu Fengyuan , Yang Xiaodong , Zhao Na , Hu Zhongyuan , Mackenzie Sally A , Zhang Mingfang , Yang Jinghua 
Published: January 2022

Abstract
Cytoplasmic male sterility (CMS) has long been used to economically produce hybrids that harness growth vigor through heterosis. Yet, how CMS systems operate within commercially viable seed production strategies in various economically important vegetable crops, and their underlying molecular mechanisms, are often overlooked details that could expand the utility of CMS as a cost-effective and stable system. We provide here an update on the nature of cytoplasmic–nuclear interplay for pollen sterility and fertility transitions in vegetable crops, based on the discovery of components of nuclear fertility restoration and reversion determinants. Within plant CMS systems, pollen fertility can be rescued by the introduction of nuclear fertility restorer genes (Rfs), which operate by varied mechanisms to countermand the sterility phenotype. By understanding these systems, it is now becoming feasible to achieve fertility restoration with Rfs designed for programmable CMS-associated open reading frames (ORFs). Likewise, new opportunities exist for targeted disruption of CMS-associated ORFs by mito-TALENs in crops where natural Rfs have not been readily identified, providing an alternative approach to recovering fertility of cytoplasmic male sterile lines in crops. Recent findings show that facultative gynodioecy, as a reproductive strategy, can coordinate the sterility and fertility transition in response to environmental cues and/or metabolic signals that reflect ecological conditions of reproductive isolation. This information is important to devising future systems that are more inherently stable.