bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pseudocontact shift NMR data obtained from a non-canonical amino acid-linked lanthanide tag improves integral membrane protein structure prediction
Authors: Ledwitch Kaitlyn V. , Künze Georg , Larochelle Katherine , Okwei Elleansar , Pankewitz Lisa , Ganguly Soumya , Darling Heather L. , Coin Irene , Meiler Jens 
Published: September 2022

AbstractA single experimental method alone often fails to provide the resolution, accuracy, and coverage needed to model integral membrane proteins (IMPs). Integrating computation with experimental data is a powerful approach to supplement missing structural information with atomic detail. We combine RosettaNMR with experimentally-derived paramagnetic NMR restraints to guide membrane protein structure prediction. We demonstrate this approach using the disulfide bond formation protein B (DsbB), an α-helical IMP. We attached a cyclen-based paramagnetic lanthanide tag to an engineered noncanonical amino acid (ncAA) using a copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry reaction. Using this tagging strategy, we collected 203 backbone HN pseudocontact shifts (PCSs) for three different labeling sites and used these as input to guide de novo membrane protein structure prediction protocols in Rosetta. We find that this sparse PCS dataset combined with 44 long-range NOEs as restraints in our calculations improves structure prediction of DsbB by enhancements in model accuracy, sampling, and scoring. The most accurate DsbB models generated in this case gave Cα-RMSD values over the transmembrane region of 2.11 Å (best-RMSD) and 3.23 Å (best-scoring).
Functional comparison of full-length palladin to isolated actin binding domain
Authors: Albraiki Sharifah , Klausmeyer Rachel , Ajiboye Oluwatosin , Beck Moriah R. 
Published: September 2022

AbstractPalladin is an actin binding protein that is specifically upregulated in metastatic cancer cells but also co-localizes with actin stress fibers in normal cells and is critical for embryonic development as well as wound healing. Of nine isoforms present in humans, only the 90 kDa isoform of palladin, comprising three immunoglobulin (Ig) domains and one proline-rich region, is ubiquitously expressed. Previous work has also established that the Ig3 domain of palladin is the minimal binding site for F-actin. In this work, we compare functions of the 90 kDa isoform of palladin to the isolated actin binding domain. To understand the mechanism of action for how palladin can influence actin assembly, we monitored F-actin binding and bundling as well as actin polymerization, depolymerization, and copolymerization. We also provide initial evidence that 90 kDa palladin exists in a closed conformation that prevents binding by the Ig3 domain to G-actin as compared to the isolated domain. Understanding the role of palladin in regulating the actin cytoskeleton may help us develop means to prevent cancer cells from reaching the metastatic stage of cancer progression.
Unsupervised identification of significant lineages of SARS-CoV-2 through scalable machine learning methods
Authors: Cahuantzi Roberto , Lythgoe Katrina A. , Hall Ian , Pellis Lorenzo , House Thomas A. 
Published: September 2022

AbstractSince its emergence in late 2019, SARS-CoV-2 has diversified into a large number of lineages and globally caused multiple waves of infection. Novel lineages have the potential to spread rapidly and internationally if they have higher intrinsic transmissibility and/or can evade host immune responses, as has been seen with the Alpha, Delta, and Omicron variants of concern (VoC). They can also cause increased mortality and morbidity if they have increased virulence, as was seen for Alpha and Delta, but not Omicron. Phylogenetic methods provide the gold standard for representing the global diversity of SARS-CoV-2 and to identify newly emerging lineages. However, these methods are computationally expensive, struggle when datasets get too large, and require manual curation to designate new lineages. These challenges together with the increasing volumes of genomic data available provide a motivation to develop complementary methods that can incorporate all of the genetic data available, without down-sampling, to extract meaningful information rapidly and with minimal curation. Here, we demonstrate the utility of using algorithmic approaches based on word-statistics to represent whole sequences, bringing speed, scalability, and interpretability to the construction of genetic topologies, and while not serving as a substitute for current phylogenetic analyses the proposed methods can be used as a complementary approach to identify and confirm new emerging variants.
Angiotensin 1-7 in severe COVID-19 patients: a phase 1 clinical trial
Authors: Valle Martins Ana Luiza , Annoni Filippo , da Silva Filipe Alex , Bolais-Ramos Lucas , Capanema de Oliveira Gisele , dos Santos Matos Alana Helen , Jardim Heyden Maria Cecília , Pinheiro Beatriz Dias , Rodrigues Natália Abdo , Alves Pereira Danilo Augusto , Lana Diniz Mirella Monique , Granato Fonseca Silva Thuanny , Cardoso Alexandre Carvalho , Martins Juliana Carvalho , Motta-Santos Daisy , Campagnole-Santos Maria José , Verano-Braga Thiago , Taccone Fabio Silvio , dos Santos Robson Augusto Souza 
Published: September 2022

AbstractBackgroundThe coronavirus-related disease (COVID-19) is mainly characterized by a respiratory involvement, with few available therapeutics for critically cases. The renin-angiotensin system (RAS) has a relevant role in the pathogenesis of COVID-19, as the virus enter host’s cells via the angiotensin-converting enzyme 2 (ACE2) and RAS disequilibrium promote inflammation and fibrosis. Exogenous angiotensin-(1-7) might modulate RAS in COVID-19 patients; however, no data on its safety are available in this setting.MethodsThis investigator-initiated, open label, phase I clinical trial was conducted to test the safety of intravenous administration of Angiotensin-(1-7) in severe COVID-19 patients admitted in two intensive care units (ICU) in Belo Horizonte, Brazil. In addition to standard of care, intravenous administration of Angiotensin-(1-7) was started at 5 mcg/Kg*day and increased to 10 mcg/Kg*day after 24 hours and continued for a maximum of 7 days or until ICU discharge. The rate of serious adverse events (SAEs) served as the primary outcome of the study.ResultsBetween August and December 2020, 28 patients were included (mean age of 55.8±12.0 years). All but one patient underwent dose escalation after 24 hours and 8 (28.5%) received the treatment until day 7. No significant differences in mean blood pressure and heart rate were observed before and after the initiation of the drug. During the period of intervention, 5/28 (17.8%) patients required vasopressors, 4 at low dose norepinephrine (i.e. <0.05 mcg/kg*min), while one patient required higher doses because of septic shock. One patient presented with sinus bradycardia, which was considered possibly related to the study drug and resolved after discontinuation. Six patients (21.4%) died before ICU discharge.ConclusionsIntravenous infusion of Angiotensin-(1-7) up to 10 mcg/Kg*day was safe in severe COVID-19 patients and could represent a potential therapeutic strategy in this setting.Trial RegistrationRegistro Brasileiro de Ensaios Clínicos, UTN code: U1111-1255-7167, registered on 08/05/2020; ClinicalTrials.gov Identifier: NCT04633772; retrospectively registered on November 18 2020
Periodic solutions for a class of perturbed sixth-order autonomous differential equations
Authors: Berrehail Chems Eddine , Makhlouf Amar 
Published: September 2022

PurposeThe objective of this work is to study the periodic solutions for a class of sixth-order autonomous ordinary differential equations x(6)+(1+p2+q2)x… .+(p2+q2+p2q2)x¨+p2q2x=εF(x,ẋ,x¨,x…,x… .,x(5)), where p and q are rational numbers different from 1, 0, −1 and p ≠ q, ε is a small enough parameter and F ∈ C2 is a nonlinear autonomous function.Design/methodology/approachThe authors shall use the averaging theory to study the periodic solutions for a class of perturbed sixth-order autonomous differential equations (DEs). The averaging theory is a classical tool for the study of the dynamics of nonlinear differential systems with periodic forcing. The averaging theory has a long history that begins with the classical work of Lagrange and Laplace. The averaging theory is used to the study of periodic solutions for second and higher order DEs.FindingsAll the main results for the periodic solutions for a class of perturbed sixth-order autonomous DEs are presenting in the Theorem 1. The authors present some applications to illustrate the main results.Originality/valueThe authors studied Equation 1 which depends explicitly on the independent variable t. Here, the authors studied the autonomous case using a different approach.
Minority shareholder activism in light of the Indian companies act, 2013
Authors: Sharma Pooja , Sachdeva Shikha 
Published: September 2022

PurposeThe genesis of “shareholder activism” in the USA may be traced back to several decades, but it only evolved in India at the start of the 21st century. This paper aims to explore the concept of “shareholder activism” in the Indian context, in light of the New Companies Act, 2013. The act is envisioned as a precursor to invoking the intention of shareholders to confront managers. Further, it aims to look at the possibilities of using tools of shareholder activism to make companies aware of their concerns.Design/methodology/approachAuthors explore the concept of shareholder activism with the help of textual analysis, using R. Then, the authors study the mediating effects of “shareholder’s intention towards activism” between the “regulatory mechanisms” and “the usage of various tools of activism”, using the partial least square approach.FindingsRegulatory mechanisms, such as the Companies Act, 2013, enhance the shareholders’ power to sensitise companies towards various corporate governance issues. It also increases their intention towards shareholder activism, eventually leading to favourable opinion on using various tools of “activism” in their investee companies.Practical implicationsThis study is a unique attempt to assess the minority shareholders’ potential to become active in their investee companies induced by changes in the rules and regulations of a country.Originality/valueShareholder activism in India has not been thoroughly explored thus far. This paper specifically studies the opinions of retail investors, who possibly could increase companies’ accountability towards their minority shareholders, especially in light of the New Companies Act, 2013.
Gender pay-gap: exploring the school-to-work transition of graduates in Italy
Authors: Ferri Valentina , García-Pereiro Thaís , Pace Roberta 
Published: September 2022

PurposeIn this article, the authors study the gender pay-gap (GPG) among graduates in Italy (2011 cohort) who were employed four years after graduation. The authors focus on individuals who are new entering in the labour market or who match a low level of experience with a high level of education.Design/methodology/approachAimed at estimating the amount of the differential between male and female average wages, the authors have applied the Oaxaca–Blinder (O–B) decomposition. The results identify the presence of a GPG at the very beginning of graduates’ careers given that, shortly after graduation, women receive lower salaries than men, even after controlling for several characteristics (individual, academic, job and local labour market). The authors completed the analysis with the reweighted O–B decomposition using the recentered influence function (RIF) and the Juhn, Murphy and Pierce and Machado and Mata decomposition approaches.FindingsThe results show that the GPG is already present at the very beginning of graduates’ careers, and it increases when correcting for women’s lower level of participation in the labour market. The authors also identified sticky floor and the glass ceiling effects due to the existence of a relevant high GPG both at the bottom and the top of the graduates’ wage distribution.Originality/valueBy focussing attention particularly on graduates, this paper adds to the existing literature a deeper understanding not only of individuals who have recently entered the labour market, but also those who are highly skilled but have little on-the-job experience. In fact, the authors are looking at a particular sample (graduates who are all transitioning from university to work during the same period) with small heterogeneity which allows the authors to compare very similar young men and women graduates and gain a deeper understanding of GPGs in early careers while controlling for confounding and hidden sources of variability.
Effect of emotional intelligence and cognitive flexibility on entrepreneurial intention: mediating role of entrepreneurial self-efficacy
Authors: Mishra Ankita , Singh Parwinder 
Published: September 2022

Purpose
Entrepreneurship is one of the significant drivers of economic growth, development and job generation in several countries worldwide. Realizing its significant contribution to the nation’s development, policymakers and educators have also drawn attention to fostering entrepreneurship among the youth. Researchers attempted to comprehend the dynamics and investigate the factors influencing entrepreneurial intention (EI). As is true for other abilities and response tendencies, individual differences exist for EI also. This study aims to explore the relationship of emotional intelligence (EIn) and cognitive flexibility (CF) with EI and mediating effect of entrepreneurial self-efficacy (ESE) in the relationship between CF, EIn and EI.

Design/methodology/approach
The cross-sectional survey was conducted to gather responses from 635 individuals aged 17–26 years (M = 19.2, SD = 1.49). The hypotheses were tested using correlation, regression and mediation analysis.

Findings
The findings indicated that EIn and CF were significantly and positively related to EI. Furthermore, ESE was found to be a partial mediator between EIn and EI and a full mediator between CF and EI.

Research limitations/implications
Results reflected the critical significance of ESE and implied that EI might be strengthened by intervening in ESE through various sources.

Originality/value
This study adds to the existing literature by incorporating less studied individual factors (EIn and CF) to better understand EI by explaining the mediation mechanism through ESE.
The economics of COVID-19: a systematic literature review
Authors: Rathnayaka Imalka Wasana , Khanam Rasheda , Rahman Mohammad Mafizur 
Published: September 2022

PurposeA systematic, PRISMA-guided literature review was conducted using four databases (ProQuest, PubMed, EconLit and Scopus) to analyze research published between February 2020 and August 2021. This review included 31 studies out of 1,248 that were identified.Design/methodology/approachIn addition to the serious health issues it causes, severe acute respiratory syndrome coronavirus 2 (COVID-19) has a destructive impact on the global economy. The objectives of this study are (1) to examine the growing literature on variations of economic factors due to COVID-19 (2) to review the literature on the governmental response to the pandemic and (3) to discover the perspective and the gaps and outline the future avenues for further research.FindingsAll selected studies (31) have used the macroeconomic, household and health economic factors to analyze the economic impacts of the COVID-19 pandemic. Among these studies, 22 articles examined the economic consequences and macroeconomic activities, 7 analyzed microeconomic costs and healthcare trade-offs and 2 studies reviewed economic uncertainty and macroeconomic expectations.Research limitations/implicationsThis study comprises the most relevant research articles to measure the economic consequences of COVID-19. As a result of the lockdown and other containment initiatives, price levels, employment and consumption patterns have all suffered.Practical implicationsTherefore, the government's requirement to develop policy tools and approaches to ensure a full recovery from the pandemic should lead to greater long-term economic resilience.Originality/valueThis study examines the economic implications of COVID-19, with the aim of not only analysing COVID-19's negative economic effects but also, those measures that provide new directions in the form of short-run economic impacts and policy decisions.
An analysis of a novel Canadian pilot health information exchange to improve transitions between hospital and long-term care/skilled nursing facility
Authors: Aird Tyler , Holditch Ceara , Culgin Sarah , Vanderheyden Margareta , Rutledge Greg , Encinareal Carlo , Perri Dan , Edward Fraser , Boyd Hugh 
Published: September 2022

PurposeThe purpose of the article is to assess the effectiveness, compliance, adoption and lessons learnt from the pilot implementation of a data integration solution between an acute care hospital information system (HIS) and a long-term care (LTC) home electronic medical record through a case report.Design/methodology/approachUtilization statistics of the data integration solution were captured at one-month post implementation and again one year later for both the emergency department (ED) and LTC home. Clinician feedback from surveys and structured interviews was obtained from ED physicians and a multidisciplinary LTC group.FindingsThe authors successfully exchanged health information between a HIS and the electronic medical record (EMR) of an LTC facility in Canada. Perceived time savings were acknowledged by ED physicians, and actual time savings as high as 45 min were reported by LTC staff when completing medication reconciliation. Barriers to adoption included awareness, training efficacy and delivery models, workflow integration within existing practice and the limited number of facilities participating in the pilot. Future direction includes broader staff involvement, expanding the number of sites and re-evaluating impacts.Practical implicationsA data integration solution to exchange clinical information can make patient transfers more efficient, reduce data transcription errors, and improve the visibility of essential patient information across the continuum of care.Originality/valueAlthough there has been a large effort to integrate health data across care levels in the United States and internationally, the groundwork for such integrations between interoperable systems has only just begun in Canada. The implementation of the integration between an enterprise LTC electronic medical record system and an HIS described herein is the first of its kind in Canada. Benefits and lessons learnt from this pilot will be useful for further hospital-to-LTC home interoperability work.
A modified model for ultimate bearing capacity of CFRP-shear-strengthened pre-cracked beams with geopolymer
Authors: Liu Jinliang , Su Xincheng 
Published: September 2022

PurposeThe effects of failure mode and strain conditions of CFRP, concrete and stirrups on the shear capacity of reinforced beams bonded by geopolymer and epoxy are studied. In addition, a prediction model of the ultimate bearing capacity of CFRP-shear-strengthened beams is proposed, which considers adhesive performance parameters adhesive performance parameter ßE and FRP width parameter ßw.Design/methodology/approachThis paper presents an experimental study on ultimate bearing capacity of CFRP-shear-strengthened pre-cracked beams with geopolymer and epoxy resin, which considers parameters such as impregnated adhesives types and CFRP-strengthened scheme.FindingsThe failure modes of CFRP-strengthened beams bonded by geopolymer are the combination of the CFRP-concrete interface substrate failure and fracture failure of CFRP, and that of epoxy is the local substrate failures with small area. The ultimate load of CFRP-strengthened beams is directly affected by the failure modes. The ultimate bearing capacity of CFRP-strengthened beams with geopolymer is 91.4% of that of epoxy resin. Compared with ultimate bearing capacity of CFRP-strengthened beams with U-shaped, that of complete-wrapping increases by 2.5%. Moreover, the stirrup peak strain is reduced by more than 30% in CFRP-strengthened beams bonded with geopolymer and epoxy resin in comparison with the unstrengthened beam. The existing prediction model cannot accurately predict the CFRP shear capacity contribution of strengthened beams with different CFRP-strengthened schemes and adhesive properties. The estimated results are much lower than the test data, and the deviation is much larger than 20%.Originality/valueGeopolymer alternative to epoxy as an adhesive is feasible and effective for CFRP reinforcement. Furthermore, the accuracy is improved by introducing parameters about adhesive properties based on the existing prediction model. The estimated results are in excellent agreement with the test data, and the deviation is controlled within −12.80%, and the model is suitable for predicting the shear capacity of FRP-strengthened beams with ßf = 90° in shear capacity database.
Responsible management education: mapping the trends, influential sources and research themes
Authors: Ahmad Nisar , Bibi Aqsa 
Published: September 2022

PurposeThis study carries out a systematic literature review (SLR) on responsible management education (RME). A total of 174 publications listed on the Scopus database addressing RME, published between 2007 and 2022 (inclusive), have been analyzed.Design/methodology/approachThe articles included in the study were searched based on relevant key terms in the title, abstract and author keywords associated with each publication. The analysis led to the identification of the most relevant sources, authors and publications that can be used to circumscribe RME. Selected studies were analyzed using the Bibliometrix R-tool.FindingsThis study shows how three interrelated levels of analysis—namely, conceptual, intellectual and social—allow researchers to further organize the data to produce rich content for the RME. The contribution of this study is twofold: first, our values-based approach helps overcome the axiological ambiguity of the principles for RME (PRME), which invoke the importance of incorporating “the values of global social responsibility” (Principle 2) but fail to define and operationalize these values. Second, the authors provide a rationale and guidance for implementing values-based RME in business schools.Originality/valueThis study offers a unique SLR on RME. It gives a clear picture of this field by talking about what has been done and what the future might hold for RME.