bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modelling osteoarthritis in mice via surgical destabilization of the medial meniscus with or without a stereomicroscope
Authors: Hu Wencheng , Lin Junqing , Wei Jiabao , Yang Yunlong , Fu Kai , Zhu Tianhao , Zhu Hongyi , Zheng Xianyou 
Published: August 2022

Aims To evaluate inducing osteoarthritis (OA) by surgical destabilization of the medial meniscus (DMM) in mice with and without a stereomicroscope. Methods Based on sample size calculation, 70 male C57BL/6 mice were randomly assigned to three surgery groups: DMM aided by a stereomicroscope; DMM by naked eye; or sham surgery. The group information was blinded to researchers. Mice underwent static weightbearing, von Frey test, and gait analysis at two-week intervals from eight to 16 weeks after surgery. Histological grade of OA was determined with the Osteoarthritis Research Society International (OARSI) scoring system. Results Surgical DMM with or without stereomicroscope led to decrease in the mean of weightbearing percentages (-20.64% vs -21.44%, p = 0.792) and paw withdrawal response thresholds (-21.35% vs -24.65%, p = 0.327) of the hind limbs. However, the coefficient of variation (CV) of weight-bearing percentages and paw withdrawal response thresholds in naked-eye group were significantly greater than that in the microscope group (19.82% vs 6.94%, p < 0.001; 21.85% vs 9.86%, p < 0.001). The gait analysis showed a similar pattern. Cartilage degeneration was observed in both DMM-surgery groups, evidenced by increased OARSI scores (summed score: 11.23 vs 11.43, p = 0.842), but the microscope group showed less variation in OARSI score than the naked-eye group (CV: 21.03% vs 32.44%; p = 0.032). Conclusion Although surgical DMM aided by stereomicroscope is technically difficult, it produces a relatively more homogeneous OA model in terms of the discrete degree of pain behaviours and histopathological grading when compared with surgical DMM without stereomicroscope. Cite this article: Bone Joint Res 2022;11(8):518–527.
Optimization of tissue adhesive curing time for surgical wound closure
Authors: Wellington Ian J. , Hawthorne Benjamin C. , Dorsey Caitlin , Connors John P. , Mazzocca Augustus D. , Solovyova Olga 
Published: August 2022

AimsTissue adhesives (TAs) are a commonly used adjunct to traditional surgical wound closures. However, TAs must be allowed to dry before application of a surgical dressing, increasing operating time and reducing intraoperative efficiency. The goal of this study is to identify a practical method for decreasing the curing time for TAs.MethodsSix techniques were tested to determine which one resulted in the quickest drying time for 2-octyle cyanoacrylate (Dermabond) skin adhesive. These were nothing (control), fanning with a hand (Fanning), covering with a hand (Covering), bringing operating room lights close (OR Lights), ultraviolet lights (UV Light), or prewarming the TA applicator in a hot water bath (Hot Water Bath). Equal amounts of TA were applied to a reproducible plexiglass surface and allowed to dry while undergoing one of the six techniques. The time to complete dryness was recorded for ten specimens for each of the six techniques.ResultsUse of the Covering, OR Lights, and Hot Water Bath techniques were associated with a 25- (p = 0.042), 27- (p = 0.023), and 30-second (p = 0.009) reduction in drying time, respectively, when compared to controls. The UV Light (p = 0.404) and Fanning (p = 1.000) methods had no effect on drying time.ConclusionUse of the Covering, OR Lights, and Hot Water Bath techniques present a means for reducing overall operating time for surgeons using TA for closure augmentation, which can increase intraoperative efficiency. Further studies are needed to validate this in vivo. Cite this article: Bone Jt Open 2022;3(8):607–610.
Freshwater gastropod community in South Konawe District, Southeast Sulawesi, Indonesia
Authors: OETAMA DEDY , PURNAMA MUHAMMAD FAJAR 
Published: August 2022

Abstract. Oetama D, Purnama MF. 2022. Freshwater gastropod community in South Konawe District, Southeast Sulawesi, Indonesia. Biodiversitas 23: 3364-3372. The freshwater gastropod population in South Konawe District, Southeast Sulawesi, Indonesia, consists of aquatic and terrestrial snail communities. This resource (gastropod) is an ecological and economic potential that needs to be maintained and utilized optimally and sustainably. This research was conducted in April-July 2021 and aimed to determine the ecological index of the inland gastropod community in the South Konawe District. The sampling location was determined qualitatively using the purposive sampling method and a simple random sampling technique (Quantitative based). Gastropod samples were collected manually (hand picking) with a paralon pipe (3.5 inches), Sieve (1 mm), and Gloves. The total gastropods obtained at 33 sampling locations in this district were 2635 individuals. The inland gastropods comprised 19 genera and 33 species spread over 9 families, namely Achantinidae, Ampullariidae, Ariophantidae, Lymnaeidae, Neritidae, Pachychilidae, Planorbidae, Thiaridae, and Viviparidae. The average abundance of gastropods reached 79.85 ind./m2 and the abundance of gastropod species ranged from 0.58-13.48 ind./m2. Overall, the analysis of gastropod ecology index showed a stable condition, where the diversity of gastropod species was categorized as high (H'= 3.03), moderate evenness (E= 0.87), and high species richness (R= 4.06). There were no dominant species (C= 0.08) and the gastropod distribution showed a clustered pattern (Ip= 0.02). These ecological index values indicated that the gastropod community in the inland waters of South Konawe District was in a stable condition. Although the gastropod community in the inland waters of the South Konawe District simultaneously has the same area as the invasive gastropod from the family Thiaridae, namely Melanoides tuberculata (O.F.Müller, 1774) and Tarebia granifera (Lamarck, 1816).
Establishing breeding house of superior sandalwood in Gunung Sewu, Indonesia: preserving the 27 selected genotypes grafted onto two types of rootstocks
Authors: RATNANINGRUM YENI W.N. , FARIDAH ENY , UTAMA ILHAM N.S. , PRASTYO BAMBANG 
Published: August 2022

Abstract. Ratnaningrum YWN, Faridah E, Utama INS, Prastyo B. 2022. Establishing breeding house of superior sandalwood in Gunung Sewu, Indonesia: preserving the 27 selected genotypes grafted onto two types of rootstocks. Biodiversitas 23: 3488-3497. Developing methods for vegetative propagation of sandalwood (Santalum album Linn.) is important for the mass production of selected genotypes. Previous research has selected the superior genotypes of sandalwood in Gunung Sewu based on seed production, crossing-ability, santalol content and/or the rare alleles characters. This study estimates the grafting compatibility of 27 selected genotypes of sandalwood, which are grafted onto two types of rootstock. In the peak rainy season in February 2021, scions were picked from the 27 genotypes originating from four landraces (Imogiri, Bejiharjo, Bleberan and Petir) in Gunung Sewu. Scions were then grafted onto two types of rootstock using the top-cleft technique. The first rootstock type is the 8 months old seedlings of the red-big-flower sandalwood variant planted in a polybag (Type 1); while the second one is the root-suckers that emerged from horizontal roots (Type 2). Results revealed Petir genotypes as the most surviving scions (100% in Type 2 and 75% in Type 1 rootstock), followed by those originating from Imogiri. All four genotypes originating from Petir (Pt1, Pt2, Pt3 and Pt4) achieved the highest scores in most of the parameters observed. The Type 2 rootstock survived more grafts (57%) compared to those of Type 1 (49%). The number of shoots and the height increment of grafting showed significant differences. The top-cleft grafting technique is considered effective in conserving the superior genotypes of sandalwood and to provide a copy of those genotypes for materials of the crossing units. The use of Petir and Imogiri genotypes as the scions, and the red-big flower variant root-suckers as the rootstocks, are highly recommended for the best grafting success.
Genetic diversity of Burmese grape (Baccaurea ramiflora Lour.) cultivars and Ha Chau cultivar identification based on DNA barcodes
Authors: KHANG DODO TAN , HUY TRAN GIA , THI NGUYEN PHAM ANH , QUYEN DOAN THI HONG , NHU NGUYEN THI BICH , MEN TRAN THANH , DUNG TRAN NHAN 
Published: August 2022

Abstract. Khang DT, Huy TG, Thi NPA, Quyen DTH, Nhu NTB, Binh BN, Men TT, Dung TN. 2022. Genetic diversity of Burmese grape (Baccaurea ramifloraLour.) cultivars and Ha Chau cultivar identification based on DNA barcodes. Biodiversitas 23: 3513-3520. Burmese grape (Baccaurea ramiflora Lour.) is an underutilized fruit tree in the Mekong Delta and has the potential for food sources as well as landscape plants,especially Ha Chau cultivar. It has been considered an indigenous plant in Can Tho city, Vietnam with specific taste. However, their genetic diversity, utilization,and identification have not been dealt with in-depth. This study aimed to characterize genetic diversity among the five cultivars of Burmese grape in the Mekong Delta and to distinguish Ha Chau from other cultivars based on DNA barcodes, namely matK, rbcL, ycf1b, rpoC1, psbK-I, atpF-H,and ITS. DNA of twelve individuals belong to five Burmese grape cultivars was extracted prior to further amplification and sequencing. Sequences were analyzed to detect variable sites and the phylogenetic tree was constructed by the Maximum Likelihood method. Based on substitution sites and indel mutations, the plastid intergenic spacer atpF-H and ycf1b gene reflected the genetic diversity in five Burmese grape cultivars. Moreover, these sequences were valuable DNA barcodes for discrimination of Ha Chau cultivar. In combination of four markers (rbcL, rpoC1, ycf1b, and psbK-I) for phylogenetic construction, our finding revealed that Ha Chau cultivar is closely related to Red cultivar with highly supported bootstrap value of 89%. Such data could be applied for reliable identification of Ha Chau cultivar from other B. ramiflora cultivars in plant authentication.
Cytogenetic study of five species of medicinal plants from Maha Sarakham Province, Thailand
Authors: SAENSOUK PIYAPORN , SAEN-IN NATKAMON , SAENSOUK SURAPON 
Published: August 2022

Abstract. Saensouk S, Saen-in N, Saensouk P. 2022. Cytogenetic study of five species of medicinal plants from Maha Sarakham Province, Thailand. Biodiversitas 23: 3593-3603. The cytogenetic study of five species of medicinal plants, namely Butomopsis latifolia (D. Don) Kunth, Iris domestica (L.) Goldblatt & Mabb., Limnocharis flava (L.) Buchenau, Murdannia loriformis (Hassk.) R.S. Rao & Kammathy, and Pontederia hastata L. from Maha Sarakham Province, northeastern Thailand was conducted. This study aimed to investigate the chromosome numbers, fundamental number (NF), karyotype forms, and perform ideogram analysis of the five species. The cytogenetic characteristics, including the chromosome number, karyology (fundamental number (NF), karyotype formula), symmetrical karyotype, chromosome size, relative length (RL), centromeric indexes (CI), and ideograms of B. latifolia (D. Don) Kunthwere studied for the first time. The chromosome numbers of five medicinal plant species were reported as 2n = 14 (B. latifolia (D. Don) Kunth)- 80 (I. domestica (L.) Goldblatt & Mabb.). Moreover, the NF had 28 (B. latifolia (D. Don) Kunth) - 80 (Iris domestica). Two species (M. loriformis(Hassk.) R.S. Rao & Kammathyand P. hastata L.) were recognized as having symmetrical karyotypes. Three species, namely B. latifolia (D. Don) Kunth, Iris domestica, and L. flava(L.) Buchenau were reported as having asymmetrical karyotypes. Chromosomes of large size (L), chromosomes of medium size (M), and chromosomes of small size (S) were observed. Ideograms of five species were provided in this study. The NF, RL, CI, chromosome size, and ideograms of all species were reported for the first time in this study. The karyotype formulae of the five species, except the karyotype formula of P. hastata L., differed from those previously studied. The cytogenetic data of the five species in this study can be used for identification.
Short Communication: Analysis of detected metabolites compounds from the crude extract of Rimau Gerga Lebong oranges fruit (Citrus reticulata ‘RGL’) using LC-QTOF-MS/MS
Authors: YUNITA ELVIRA , KURNIATI TRI , ROSA FEBY LILIA , MELATI PUTJHA , LESTARI NOVRIANTIKA 
Published: August 2022

Abstract.Yunita E, Kurniati T, Rosa FL, Melati P, LestariN. 2022. Short Communication: Analysis of detected metabolites compounds from the crude extract of Rimau Gerga Lebong orangesfruit (Citrus reticulata ‘RGL’) using LC-QTOF-MS/MS. Biodiversitas 23: 3778-3783. Rimau Gerga Lebong (RGL) oranges are the leading commodity in Bengkulu Province. However, the content of bioactive compounds in RGL oranges has not been widely reported. This study aims to identify the flavonoid, alkaloids, and tannins from the ethanol extract of RGL oranges. The results of further identification will be used as a reference in further research on molecular docking in various biological activities. Chemical compounds were identified using qualitative tandem liquid chromatography quadrupole time of flight mass spectrometry (LC-QTOF-MS/MS). The measurement of vitamin C levels was carried out using High-Performance Liquid Chromatography (HPLC). The results showed 25 identified compounds, consisting of 6 compounds belonging to the alkaloid group, 17 from the flavonoid group, and 2 from the tannin group. The alkaloid group compounds were 2?, 3?, 6exo-trihydroxy nortopane, 2?, 3?-dihydroxy nortopane, isosalsolin,3?-dihydrocadambine, chelidimerine, and oleracein D. The compounds from identified flavonoid group were chebuloside, neokurarinol, rutinoside, flavonone, glucoside, licochalcone, viscumneoside, and pinnatifinoside. The identified tannins are corilagin and moracin. These compounds that have been identified have biological activity potential as antioxidants, anti-asthma, anti-dementia, and anticancer. The vitamin C content of the ethanol extract of RGL oranges was 0.02±0.0001%. This vitamin C supports the antioxidant activity of RGL orange.
Chronotropic incompetence in end-stage liver disease
Authors: Główczyńska Renata , Borodzicz-Jażdżyk Sonia , Peller Michał , Raszeja-Wyszomirska Joanna , Milkiewicz Piotr , Zieniewicz Krzysztof , Opolski Grzegorz 
Published: August 2022

Background
Cirrhosis causes alterations in the cardiovascular and autonomic nervous systems and leads to cirrhotic cardiomyopathy (CCM). CCM is defined as cardiac dysfunction characterized by an impaired systolic responsiveness to stress or exercise, and/or impaired diastolic function, as well as electrophysiological abnormalities, including chronotropic incompetence (CI), in the absence of other known cardiac disease. CI is a common feature of autonomic neuropathy in cirrhosis. The aim of the study is to assess the role of cardiac exercise stress test in the diagnosis of CCM.

Methods
The analysis included 160 end-stage liver disease (ESLD) patients who underwent a cardiac exercise stress test prior to the orthotopic liver transplantation. CI was defined as the inability to achieve the heart rate reserve (HRR). Pertaining to the therapy with beta-blockers: 80% of HRR was achieved in patients not taking beta-blockers and 62% in patients taking beta-blockers.

Results
In the analyzed population, 68.8% of patients met the criteria for CI. CI was more frequent in the more severe ESLD (with a higher MELD score and in a higher Child-Pugh class). In comparison to the viral hepatitis and other etiologies of ESLD, patients with alcoholic cirrhosis had a significantly lower rest heart rate (HR), lower maximal HR, lower median achieved percentage of maximal predicted HR (MPHR), a smaller percentage of patients achieved ≥ 85% of MPHR and a lower heart rate reserve. No significant relationship between the survival of OLT recipients and presence of chronotropic incompetence regarding to class of Child-Pugh scale, MELD score and etiology of ESLD were found.

Conclusions
The prevalence of CI is higher among liver transplant candidates than previously described. The altered chronotropic response may differ in regard to the severity of liver disease correlating with both the Child-Pugh and MELD scores, however CI does not seem to influence the long-term survival post OLT. Exercise stress test is a reliable, safe and useful tool for the diagnosis of CCM in liver transplant candidates and should be included in the standard cardiovascular assessment prior to OLT.
Could ultrasound midwifery training increase antenatal detection of congenital anomalies in Ghana?
Authors: Abdul-Mumin Alhassan , Rotkis Lauren N. , Gumanga Solomon , Fay Emily E. , Denno Donna M. 
Published: August 2022

Background
As part of World Health Organization (WHO) 2016 updated antenatal care (ANC) guidelines routine ultrasonography is recommended, including to detect congenital anomalies. The Ghana Health Service (GHS) developed an in-service midwifery ultrasound training course in 2017, which includes fetal anomaly detection. Training rollout has been very limited. We sought to determine proportions of anomalies among neonates presenting to Tamale Teaching Hospital (TTH) that should be prenatally detectable by course-trained midwives in order to determine training program potential utility.

Methods
We analyzed data from a registry of neonates admitted to TTH with congenital anomaly diagnoses in 2016. We classified ultrasonographic detectability of anomalies at ≤13 and 14–23 weeks gestation, based on GHS course content and literature review. Secondary analysis included 2011–2015 retrospective chart review data.

Results
Eighty-five neonates with congenital anomalies were admitted to TTH in 2016. Seventy-three (86%) mothers received ≥1 ANC visit; 47 (55%) had at least one prenatal ultrasound, but only three (6%) were interpreted as abnormal. Sixteen (19%) and 26 (31%) of the anomalies should be readily detectable by course-trained midwives at ≤13 and 14–23 weeks gestation, respectively. When the 161 anomalies from 2011–2015 were also analyzed, 52 (21%) and 105 (43%) should be readily detectable at ≤13 and 14–23 weeks gestation, respectively. “Optimal conditions” (state-of-the-art equipment by ultrasonography-trained physicians) should readily identify 53 (22%) and 115 (47%) of the anomalies at ≤13 and 14–23 weeks gestation, respectively.

Conclusion
Training Ghanaian midwives could substantially increase second trimester anomaly detection, potentially at proportions nearing highly resourced settings. Our data also highlight the need for refinement of the WHO antenatal ultrasonography recommendation for a scan before 24 weeks gestation for multiple purposes. Gestational dating accuracy requires first trimester scanning while fetal anomaly detection is more accurate during second trimester. Further specification will enhance guideline utility.
Analysis of mobility level of COVID-19 patients undergoing mechanical ventilation support: A single center, retrospective cohort study
Authors: Nawa Ricardo Kenji , Serpa Neto Ary , Lazarin Ana Carolina , da Silva Ana Kelen , Nascimento Camila , Midega Thais Dias , Caserta Eid Raquel Afonso , Corrêa Thiago Domingos , Timenetsky Karina Tavares 
Published: August 2022

Background
Severe coronavirus disease 2019 (COVID-19) patients frequently require mechanical ventilation (MV) and undergo prolonged periods of bed rest with restriction of activities during the intensive care unit (ICU) stay. Our aim was to address the degree of mobilization in critically ill patients with COVID-19 undergoing to MV support.

Methods
Retrospective single-center cohort study. We analyzed patients’ mobility level, through the Perme ICU Mobility Score (Perme Score) of COVID-19 patients admitted to the ICU. The Perme Mobility Index (PMI) was calculated [PMI = ΔPerme Score (ICU discharge–ICU admission)/ICU length of stay], and patients were categorized as “improved” (PMI > 0) or “not improved” (PMI ≤ 0). Comparisons were performed with stratification according to the use of MV support.

Results
From February 2020, to February 2021, 1,297 patients with COVID-19 were admitted to the ICU and assessed for eligibility. Out of those, 949 patients were included in the study [524 (55.2%) were classified as “improved” and 425 (44.8%) as “not improved”], and 396 (41.7%) received MV during ICU stay. The overall rate of patients out of bed and able to walk ≥ 30 meters at ICU discharge were, respectively, 526 (63.3%) and 170 (20.5%). After adjusting for confounders, independent predictors of improvement of mobility level were frailty (OR: 0.52; 95% CI: 0.29–0.94; p = 0.03); SAPS III Score (OR: 0.75; 95% CI: 0.57–0.99; p = 0.04); SOFA Score (OR: 0.58; 95% CI: 0.43–0.78; p < 0.001); use of MV after the first hour of ICU admission (OR: 0.41; 95% CI: 0.17–0.99; p = 0.04); tracheostomy (OR: 0.54; 95% CI: 0.30–0.95; p = 0.03); use of extracorporeal membrane oxygenation (OR: 0.21; 95% CI: 0.05–0.8; p = 0.03); neuromuscular blockade (OR: 0.53; 95% CI: 0.3–0.95; p = 0.03); a higher Perme Score at admission (OR: 0.35; 95% CI: 0.28–0.43; p < 0.001); palliative care (OR: 0.05; 95% CI: 0.01–0.16; p < 0.001); and a longer ICU stay (OR: 0.79; 95% CI: 0.61–0.97; p = 0.04) were associated with a lower chance of mobility improvement, while non-invasive ventilation within the first hour of ICU admission and after the first hour of ICU admission (OR: 2.45; 95% CI: 1.59–3.81; p < 0.001) and (OR: 2.25; 95% CI: 1.56–3.26; p < 0.001), respectively; and vasopressor use (OR: 2.39; 95% CI: 1.07–5.5; p = 0.03) were associated with a higher chance of mobility improvement.

Conclusion
The use of MV reduced mobility status in less than half of critically ill COVID-19 patients.