bluuebunny/crossref_metadata_2025_split huggingface dataset:
A time-resolved fluorescence immunoassay for rapid and precise automatic quality control of human papillomavirus type 68 VLPs in human papillomavirus vaccine
Authors: Li Zhaoyue , Chen Yin , Li Yang , Zhang Zhigao , Zhai Xiangming , Cao Yue , Li Xijiu , Yang Yiqi , Wu Yingsong , Lin Guanfeng 
Published: October 2022

Abstract

The effectiveness and necessity of human papillomavirus (HPV) vaccination to prevent HPV infection and cervical cancer are increasingly recognized by people. The 15-valent HPV vaccine, which protects against almost high-risk types of HPV viruses identified by WHO, has attracted much attention. However, as the valence of vaccines increases, quality control in the HPV vaccine production process is facing more challenges. The precise quality control of the HPV type 68 virus-like particles (VLPs), one of the unique components of the 15-valent HPV vaccine that distinguishes it from existing vaccines, is the new requirement for vaccine manufacturers. Here we developed a novel time-resolved fluorescence immunoassay (TRFIA) for rapid and precise automatic quality control of HPV68 VLPs in HPV vaccine. Two murine monoclonal antibodies specifically targeting the HPV68 L1 protein were used to establish a classical sandwich assay. Except for pretreating the vaccine sample, the whole analysis process was performed by a fully automated machine, which saves detection time and gets rid of manual error. Multiple experiments established that the current novel TRFIA can efficiently and reliably analyses HPV68 VLPs. Present novel TRFIA has exhibited merits with speed, robustness, high sensitivity with a minimum detection value of 0.08 ng/mL, considerable accuracy, a wide detection range (up to 1000 ng/mL) and excellent specificity. It is also expected to provide a new detection method for quality control for each HPV type VLPs. To summarize, the novel TRFIA is of great interest for application in HPV vaccine quality control.
The Antiviral Activity of Triple Motif Protein 38 In Hepatitis B Virus Replication and Gene Expression and Its Association with Treatment Responses During PEG-IFN-α Antiviral Therapy
Authors: Luo Haiying , Hu Xiaoxia , Li Yadi , Lei Dingjia , Tan Guili , Zeng Yueying , Qin Bo 
Published: October 2022

Abstract
Background
Hepatitis B virus (HBV) infection is the most critical factor underlying liver cirrhosis and hepatocellular carcinoma worldwide.The triple motif protein 38 (TRIM38) is an interferon-stimulated gene (ISG) that can indirectly inhibit various DNA and RNA viruses by modulating the type I interferon response.However, the relationship between TRIM38 and HBV infection and therapy is yet to be elucidated.Our study aims to investigate the correlation between TRIM38 expression levels and the efficacy of HBV infection and IFN-α therapy in patients with CHB.
Methods
TRIM38 was overexpressed or knocked down in human hepatoma cells and the cells and supernatant were collected.The levels of HBV RNA, pgRNA and supernatant antigen were detected by qRT-PCR or ELISA to evaluate the inhibitory effect of TRIM38 on HBV.Blood samples of CHB patients who received pegylated interferon-α(PEG-IFN-α) therapy were collected, and PBMC was isolated.The alternation in the gene expression level of TRIM38 was detected by qRT-PCR, and the predictive value of TRIM38 changes during early therapy was evaluated.The induction of antiviral proteins was analyzed by immunoblotting.
Results
In human hepatoma cells, TRIM38 was highly induced by IFN-alpha (IFN-α) and enhanced anti-HBV activity.Furthermore, combined treatment with TRIM38 and IFN-α increased antiviral proteins levels.The overexpression of TRIM38 inhibited while knockdown of TRIM38 elevated HBV replication and gene expression in HepG2 and HepG2.2.15 cells.TRIM38 is negatively correlated with chronic HBV infection.Prospective study showed that high levels of TRIM38 in peripheral blood PBMCs were observed in the early responders, and higher TRIM38 expression co-related with a better response to PEG-IFN-α therapy.
Conclusions
Taken together, our study suggested that TRIM38 plays a vital role in HBV replication and gene expression and TRIM38 may become a new target for the treatment of HBV.
The settlement phase in the common octopus Octopus vulgaris: a complex transition between planktonic and benthic lifestyles
Authors: Roura Alvaro , Castro-Bugallo Alexandra , Martínez-Pérez Miguel 
Published: October 2022

Abstract
Planktonic octopuses undergo a transitional period from a pelagic lifestyle to the predominantly benthic life of the juvenile stage, known as settlement, that is scarcely documented in the wild or captivity. In this work two generations of the common octopus, Octopus vulgaris, were reared in captivity and three different stages were defined for the settlement phase based on morphological, anatomical, and behavioural changes: pre-settlement or “tactile” stage, settlement, and post-settlement or “ninja” stage. Before settlement the swimming planktonic paralarvae are transparent with 65–80 chromatophores, iridophores covering eyes and digestive system, Kölliker organs, circular pupils, ~ 20 suckers, and mantle length (ML) bigger than total length (TL; ML/TL > 60%). The “tactile” stage (ML/TL from 65 − 55% and ~ 20–25 suckers) is marked by the onset of pre-settlement reflexes, where the late paralarvae touch the walls and bottom of the tank and start crawling clumsily. Morphologically, the paralarvae are transparent with increasing chromatophores and iridophores along the arms and the dorsal area of head and mantle. During the settlement stage (~ 55 − 48% ML/TL, and ~ 25–35 suckers), there is an exponential increase of chromatophores in the dorsal area and a marked change in behaviour, with paralarvae showing strong negative phototaxis, looking for shelter when disturbed. The skin is still transparent but new chromatic cells (leucophores) start to develop and the Kölliker organs are almost lost. During the post-settlement stage (~ 48 − 40% ML/TL, > 35 suckers) the chromatic cells keep increasing exponentially, giving a pale look to the skin. The early juveniles have horizontal pupils, with the “eye-bar” and display very fast and coordinated movements like “ninjas”. The start of the benthic phase is marked by the presence of skin sculptural components (papillae and cirrha) and the display of complex chromatic and body patterns.
The association of menopausal hormone therapy with the incidence of urinary tract cancer: a national population-based study
Authors: Yuk Jin-Sung , Yoon Sang-Hee , Yu Ji Hyeong , Kim Jae Yoon 
Published: October 2022

Abstract

This study evaluates the relationship between menopausal hormone therapy (MHT) and the risk of urologic cancer in women. It was conducted for South Korea’s national population based on the National Health Insurance Service Database between January 2002 and January 2019. The types of MHT in this study included tibolone, combined oestrogen plus progestin by the manufacturer (CEPM) or physician (CEPP), and oral and topical oestrogen. Furthermore, select patient characteristics and reproductive factors were reviewed. We performed a Cox proportional hazard analysis to clarify the risk of urologic cancer associated with MHT. According to MHT types, 104,089 were treated with tibolone, 65,597 with CEPM, 29,357 with oral oestrogen, 3,913 with CEPP, and 1,174 with topical oestrogen. Among women on MHT, the incidence of kidney cancer was significantly increased with oral oestrogen (hazard ratio [HR] 1.36, 95% confidence interval [CI]: 1.062–1.735) and topical oestrogen (HR 2.84, 95% CI: 1.270–6.344), whereas other formulations were not associated with kidney cancer. Meanwhile, tibolone significantly decreased the incidence of bladder cancer (HR 0.69, 95% CI: 0.548–0.858), whereas other formulations were not associated with bladder cancer. Our findings suggest that MHT in postmenopausal women affects the incidence of urologic cancers.
Studies on Entire Span Vibration and Local Deformation Characteristicsof Transmission Line under Impact Load
Authors: Zhu He , Chen Wenlong , Han Zhaobing , Liu Cheng , Yang Junqing , Chen Huan 
Published: October 2022

Abstract
In order to study the entire span vibration and local deformation characteristics of transmission conductor under impact load collision, the mathematical model of entire span vibration and local deformation of transmission conductor is established. Considering the influence of conductor local deformation and conductor steel and aluminum strand on Poisson's ratio and elastic modulus, the entire span impact vibration simulation model and local impact deformation simulation model of transmission conductor are established, The macroscopic entire span vibration and local deformation of transmission line are analyzed. The results show that the maximum sag of each position on the transmission line occurs when the impact force acts on that position; The change of impact force has the greatest impact on the maximum value of sag near the suspension point and the least impact on the maximum value of sag near the center of span; Impact has the greatest impact on the outermost aluminum strand. With the deepening of impact, the deformation of each layer decreases gradually, but the deformation area increases gradually; The influence of impact velocity on the deformation of inner aluminum strand increases gradually, but the influence on the deformation of inner steel core is basically unchanged.
Autobiographical memory specificity and restrained eating: examining the influence of priming with images of healthy and unhealthy foods.
Authors: Wallis Deborah Jane , Moss Jessica , Varnam Bethany , Dritschel Barbara , Ridout Nathan 
Published: October 2022

Abstract

Background: Dietary restraint has been linked to deficits in the ability to recall detailed memories of personally experienced events (referred to as autobiographical memory specificity). As priming with healthy foods increases the salience of restraint it would be expected to lead to greater deficits in memory specificity.
Objective: To determine if priming word cues with images of healthy or unhealthy foods would influence the specificity of memory retrieval, and if deficits in memory specificity would be more evident in those reporting higher levels of dietary restraint, or currently dieting.
Methods: Sixty female undergraduates self-reported if they were currently dieting and completed measures of mood, restraint, and disinhibition, and a modified version of the autobiographical memory task. Participants were presented with positive and negative words (unrelated to eating concerns) and asked to retrieve a specific memory in response to each cue. A food image was shown prior to each word cue; half of the participants were primed with images of healthy foods and half with images of unhealthy foods.
Results: As expected, participants primed with healthy foods retrieved fewer specific memories than did those primed with unhealthy foods. However, neither restraint nor current dieting behaviour were associated with memory specificity.
Conclusions: Differences in memory specificity between the priming conditions cannot be explained in terms of increased salience of restraint. However, it is plausible that unhealthy images led to an increase in positive affect, which in turn improved memory specificity.
Level of evidence: Level I: Evidence obtained from: at least one properly designed experimental study
Genomic Variability Correlates with Biofilm Phenotypes in Multidrug Resistant Clinical Isolates of Pseudomonas aeruginosa
Authors: Islam Ovinu Kibria , Islam Israt , Saha Otun , Sultana Munawar , Bockmuhel Dirk , Hossain M Anwar 
Published: October 2022

Abstract
The multifactorial nature of Pseudomonas aeruginosa biofilm development and genomic variabilities implicates its resistance to conventional antimicrobials and virulence. Therefore, genetic determinants need to be extensively studied to block the early steps of biofilm or already formed biofilms. In this study, a total of 20 multidrug resistant (MDR) clinical P. aeruginosa isolates were evaluated for their biofilm forming abilities and related genes. Of the isolates tested, all of them showed surface attachment tendencies in nutrient limiting conditions, and classified as strong (SBF=45%), moderate (MBF=30%) and weak (WBF=25%) biofilm formers. Complete genome sequencing of representative strong (DMC-27b), moderate (DMC-20c) and weak biofilm former (DMC-30b) isolates was performed. Analysis of biofilm related genes in the sequenced genomes revealed that,80 of the 88 biofilm related genes possess 98–100% sequence identity to the reference PA01 strain. Complete and partial sequence data of LecB proteins from tested isolates indicate that isolates containing PA14-like LecB sequences produced strong biofilms. All of the 7 pel operon protein coding genes in weak biofilm former isolate 30b showed significant nucleotide sequence variation with other tested isolates, and their corresponding proteins are 99% identical with the pel operon proteins of PA7. Bioinformatics analyses identified divergent sequence and structural features that separate PA7 like pel operon proteins from reference PAO1 like pel operon. Congo red and pellicle forming assays revealed that the sequence and structure variations may have interfered with the Pel production pathway and resulted in impaired Pel production in isolate 30b that has a PA7 like pel operon. Expression analysis also showed that both PelB and LecB proteins were about 5 to 6 folds upregulated after 24 hours in SBF 27b in comparison with WBF 30b. Our findings indicate significant genomic divergence in biofilm related genes of P. aeruginosa strains that affect their biofilm phenotypes.
Evidence of a compensatory response in invasive Rusty Crayfish (Faxonius rusticus) following intensive harvest removal from northern Lake Michigan fish spawning reefs
Authors: Kvistad Jake T , Galarowicz Tracy L. , Clapp David F. , Chadderton W. Lindsay , Tucker Andrew J. , Herbert Matthew 
Published: October 2022

Abstract
The goal of a harvest removal program to control invasive species is usually to achieve sustained reductions in population abundance. Yet removal efforts may be counteracted by density-dependent processes by increasing available resources and reducing stage-specific mortality. We tested a harvest removal strategy for invasive Rusty Crayfish (Faxonius rusticus) focused on nearshore cobble reefs, used by several native fishes as spawning habitats, in Lake Michigan with a before-after control-impact (BACI) study design. We removed 3182 Rusty Crayfish, primarily adults (> 20 mm carapace length), at two sites over two harvest seasons, expending 17,825 trap days in effort. While the BACI analysis results suggested that a statistically significant reduction in Rusty Crayfish densities assessed from diver quadrat surveys was achieved on one reef (Little Traverse Bay Crib), suppression effects were highly variable across sites. In addition, reduced densities were sustained over the egg maturation period for native fish and into the following year after removal ceased. However, by late summer/early fall between consecutive suppression efforts in 2018 and 2019 we observed a threefold increase in pre-removal densities. Size-frequency histograms from diver quadrat surveys showed higher abundance of juvenile size classes over the following spring and summer at Little Traverse Bay Crib compared to its paired reference site, suggesting a compensatory response. Evidence of compensation was corroborated by stock-recruitment analysis and a coincident increase in ovarian egg counts at Little Traverse Bay Crib between 2018 and 2019. With a proviso that we only conducted two seasons of consecutive suppression, our results emphasizes the effects of density-dependent processes in invasive species management and raises questions about efficacy of adult-only removal strategies.
Wetlands Mapping with Deep ResU-Net CNN and Open-Access Multisensor and Multitemporal Satellite Data in Alberta’s Parkland and Grassland Region
Authors: Onojeghuo Alex Okiemute , Onojeghuo Ajoke Ruth 
Published: October 2022

Abstract
Wetlands are a valuable ecosystem that provides various services to flora and fauna. This study developed and compared deep and shallow learning models for wetland classification across the climatically dynamic landscape of Alberta’s Parkland and Grassland Natural Region. This approach to wetland mapping entailed exploring multi-temporal (combination of spring/summer and fall months over four years – 2017 to 202) and multisensory (Sentinel 1 and 2 and Advanced Land Observing Satellite - ALOS) data as input in the predictive models. This input image consisted of S1 dual-polarization vertical-horizontal bands, S2 near-infrared and shortwave infrared bands and ALOS-derived Topographic Wetness Index. The study explored the ResU-Net deep learning (DL) model and two shallow learning models, namely random forest (RF) and support vector machine (SVM). We observed a significant increase in the average F1-score of the ResNet model prediction (0.77) compared to SVM and RF prediction of 0.65 and 0.64, respectively. The SVM and RF models showed a significant occurrence of mixed pixels, particularly marshes and swamps confused for upland classes (such as agricultural land). Overall, it was evident that the ResNet CNN predictions performed better than the SVM and RF models. The outcome of this study demonstrates the potential of the ResNet CNN model and exploiting open-access satellite imagery to generate credible products across large landscapes.
Clinician perspectives on offering services to adolescents with mental health problems in a National Referral Hospital in Kenya
Authors: Wambua Grace Nduku , Kumar Manasi , Falkenström Fredrik , Cuijpers Pim 
Published: October 2022

Abstract
Background: The aim of this paper was to explore clinicians’ perspectives on the provision of psychotherapy to adolescents referred for care in a public health facility, with a focus on identifying both facilitators and barriers to the treatment process.
Method: The study was conducted at the Youth Clinic of the Mental Health Department at Kenyatta National Hospital (KNH) in Nairobi. Ten clinicians participated in the interviews. Interviews were recorded and transcribed verbatim for coding. A reflexive thematic approach was utilized for analysis.
Findings: Our analysis identified five overarching but interrelated themes. The clinicians describe different facilitators and barriers to the therapeutic process within each theme. They included (i) awareness of mental health issues was limited among adolescents and caregivers with the internet offering new source of information for adolescents, (ii) community awareness of available help was also inadequate with adolescents and caregivers often preferring a quick solution as opposed to time-consuming therapy, (iii) dose of treatment and perception of adequacy identified as conflict areas for all stakeholders involved, with 3 sessions often being prescribed by the schools, with clinicians describing them as inadequate, (iv) premature termination of the process which was influenced by user perceptions and attitudes toward treatment and finally (v) the clinicians identified that a good therapeutic relationship was important for good therapeutic outcomes.
Discussion: Our findings give us insight into the therapeutic process, giving an understanding of some of the barriers and facilitators as experienced by clinicians working with adolescents in Kenya. Issues such as low mental health literacy have greater impact on not only help-seeking behaviors in the group, but appropriate utilization of services for their benefit. This work yields important insights that can be used to improve adolescent mental health services.
Childhood maltreatment and emotion regulation in everyday life: An experience sampling study
Authors: Miu Andrei C. , Bîlc Mirela I. , Szentágotai-Tătar Aurora , Pop Claudia Felicia , Ion Andrei 
Published: October 2022

Abstract
Childhood maltreatment is a major risk factor for psychopathology, and increasing evidence suggests that emotion regulation (ER) is one of the underlying mechanisms. However, most of this evidence comes from cross-sectional studies and single assessments of habitual ER strategies. In the present study, we investigated the relation between history of childhood maltreatment, positive and negative affect, and multiple dimensions of spontaneous ER (strategy use, ER success, effort, and goals) in everyday life, using experience sampling method (3 assessments/day, for 10 consecutive days), in a sample of healthy volunteers (N = 118). Multilevel modeling results indicated that childhood maltreatment was associated with lower positive affect and higher negative affect. Childhood maltreatment was also related to lower use of reappraisal and savoring (but not suppression, rumination and distraction), reduced ER success (but not effort), as well as lower levels of and higher within-individual variability of hedonic (but not instrumental) ER goals. These results provide ecological evidence that individuals with a history of childhood maltreatment are characterized by differences in multiple ER dimensions.
Adverse cardiovascular, limb, and renal outcomes in patients with diabetes after peripheral artery disease revascularization treated with sodium glucose cotransporter 2 inhibitors versus dipeptidyl peptidase-4 inhibitors Cover title: SGLT2i versus DPP4i in T2D patients after PAD revascularization
Authors: Lee Hsin-Fu , Chuang Chi , Li Pei-Ru , Yeh Yung-Hsin , Chan Yi-Hsin , See Lai-Chu 
Published: October 2022

Abstract
Aims
The effectiveness and limb safety of sodium glucose co-transporter 2 inhibitors (SGLT2i) for patients with type-2 diabetes (T2D) who have received peripheral artery disease (PAD) revascularization are unknown.
Methods and results
In this nationwide retrospective cohort study, we identified a total of 2,455 and 8,695 patients with T2D who had undergone PAD revascularization and received first prescriptions for SGLT2i and dipeptidyl peptidase-4 inhibitors (DPP4i), respectively, between May 1, 2016, and December 31, 2019. We used 1:1 propensity score matching (PSM) to balance covariates between the two study groups. Patients were followed up from the drug index date until the occurrence of specified outcomes, death, discontinuation of the index drug, or the end of the study period, whichever occurred first. After PSM, we observed that compared with DPP4i, SGLT2i were associated with comparable risks of ischemic stroke, acute myocardial infarction, and heart failure hospitalization but were associated with a lower risk of cardiac death (hazard ratio [HR]: 0.60; 95% confidence interval [CI]: 0.40–0.90]; p = 0.0126). Regarding major limb outcomes, SGLT2i were associated with comparable risks of repeated revascularization and lower limb amputation compared with DPP4i. SGLT2i were associated with a lower risk of composite renal outcomes (HR: 0.40; 95% CI: 0.27–0.59; p < 0.0001) compared with DPP4i.
Conclusion
In a real-world study of patients with T2D who had undergone PAD revascularization, SGLT2i were associated with lower risks of cardiac death and composite renal outcomes but not associated with increased risks of adverse limb eventscompared with DPP4i.