bluuebunny/crossref_metadata_2025_split huggingface dataset:
Healthy eating and all-cause mortality among Chinese aged 80 years or older
Authors: Yan Lijing L. , Li Chaoyun , Zou Siyu , Li Yaxi , Gong Enying , He Zhengting , Shao Shuai , Jin Xurui , Hua Yechu , Gallis John A. , Turner Elizabeth L. 
Published: May 2022

Abstract
Background
There is little evidence of the influence of dietary patterns on mortality risk among adults 80 years or older (“oldest-old”). We evaluated the association between the Simplified Healthy Eating index (SHE-index) and mortality among Chinese oldest-old.

Methods
Population-based cohort study from the Chinese Longitudinal Healthy Longevity Survey (CLHLS 1998–2014, n = 35 927), conducted in 22 Chinese provinces, were pooled for analysis. The first seven waves of the CLHLS (1998, 2000, 2002, 2005, 2008–09, 2011–12, and 2013–2014) were utilized, with follow-up to the last wave (2018) (range 0–21 years). The SHE-index was collected in each wave, and was constructed from intake frequency of nine dietary variables, with a higher score indicating better diet quality. Cox proportional hazards model with dietary patterns as a time-varying exposure was employed to analyze the relationship between SHE-index and mortality.

Results
At baseline, the median age of all participants was 92 years (25th percentile, 85 years; 75th percentile, 100 years). In multivariable models, the hazard ratios (95% confidence intervals) for SHE-index quartile 2, quartile 3 and quartile 4 versus quartile1 were 0.91 (0.88, 0.93), 0.89 (0.86, 0.92) and 0.82 (0.78, 0.85), respectively. Results were generally consistent for men and women and in a large number of sensitivity analyses.

Conclusions
Healthier eating patterns were associated with a significant reduction in the risk of all-cause mortality among Chinese oldest-old, lending support to the importance of life-long adherence to healthy diet into advanced old age.
DLEU1 promotes cell survival by preventing DYNLL1 degradation in esophageal squamous cell carcinoma
Authors: Li Qihang , Zhang Zhiyu , Jiang HongChao , Hou Jun , Chai Yuhang , Nan Hongxing , Li Feng , Wang Lianghai 
Published: May 2022

Abstract
Background
Emerging evidence has highlighted the critical roles of long noncoding RNAs (lncRNAs) in tumor development and progression. However, the biological functions and underlying mechanisms of DLEU1 in esophageal squamous cell carcinoma (ESCC) remain unclear.

Methods
LncRNA expression in ESCC tissues was explored using lncRNA microarray datasets. The functional roles of DLEU1 in ESCC were demonstrated by a series of in vitro and in vivo experiments. RNA pull-down and immunoprecipitation assays were performed to demonstrate the potential mechanisms of DLEU1.

Results
In a screen for differentially expressed lncRNAs in ESCC, we determined that DLEU1 was one of the most overexpressed lncRNAs in ESCC tissues and that upregulated DLEU1 expression was associated with a worse prognosis. Functional assays showed that DLEU1 promoted tumor growth by inhibiting cell apoptosis. Mechanistically, DLEU1 could bind and stabilize DYNLL1 by interfering with RNF114-mediated ubiquitination and proteasomal degradation. The DLEU1/DYNLL1 axis subsequently upregulated antiapoptotic BCL2 and promoted cell survival. Furthermore, DLEU1 upregulation was at least partly facilitated by promoter hypomethylation. Notably, targeting DLEU1 sensitized ESCC cells to cisplatin-induced death.

Conclusions
Our findings suggest that DLEU1-mediated stabilization of DYNLL1 is critical for cell survival and that the DLEU1/DYNLL1 axis may be a promising therapeutic target for ESCC.
Anti-L1 antibody-bound HPV16 pseudovirus is degraded intracellularly via TRIM21/proteasomal pathway
Authors: Li Meiying , Huang Jianmei , Zhu Yi , Huang Ziyi , Zhang Guonan , Huang Jianming 
Published: May 2022

Abstract
Background
Persistent HPV16 infection is the leading risk factor for developing cervical cancer. Anti-L1 antibodies against HPV16 produced in HPV16 infections play diverse roles in the clearance of virus infection and prevention of persistence. It has been implicated that the cervicovaginal squamous epithelial cells actually express TRIM21 and that some HPV16 particles could escape leaky endosomal compartment into the cytosol and that Fc receptor TRIM21 directly neutralize infection by targeting antibody-opsonized viruses for proteasomal degradation. We explored whether anti-L1 antibody opsonized HPV16 pseudovirus (PsV) entered into the cytosol could be neutralized by TRIM21-mediated activation of a proteasomal pathway to reduce the chance of persistent HPV16 infection.

Methods
HPV16 PsV were generated and extracted in HEK 293FT cells co-transfected with pcDNA3.1-eGFP and p16sheLL plasmids according to the standard protocol. The HPV16 PsV with capsid protein L1 was characterized by fluorescence microscopy and western blot, and the HPV16 PsV titer and anti-L1-bound PsV entry efficiency were detected by flow cytometry. The expressions of transcription factors (TF) and cytokines elicited by the TRIM21-activated proteasomal pathway were confirmed by dual-luciferase reporter assay and RT-qPCR. The changes in HPV16 PsV load with or without inhibitors in the infected HEK 293FT cells were determinated by qPCR.

Results
Simultaneous transfection with pcDNA3.1-eGFP and p16sheLL plasmids into the HEK 293FT cells resulted in the self-assembly of HPV16 PsV with capsid protein L1. Both HPV16 PsV and anti-L1-bound HPV16 PsV could infect HEK 293FT cells. Anti-L1-bound PsV up-regulated TRIM21 mediated-activation of proteasome and increased expressions of TF and cytokines in the infected cells where HPV16 PsV load reduced by ~ 1000-fold in the presence of anti-L1 antibody, but inhibition of proteasomal activity increased HPV16 PsV load.

Conclusion
Our preliminary results indicate that anti-L1 antibody entered with HPV16 PsV into the cells could mediate degradation of HPV16 PsV by TRIM21-activated proteasomal pathway intracellularly, giving anti-capsid protein L1 antibody a role in host defense of persistent HPV16 infection.
MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein–protein interactions
Authors: Velay Florent , Soula Mélanie , Mehrez Marwa , Belbachir Clément , D’Alessandro Stefano , Laloi Christophe , Crete Patrice , Field Ben 
Published: May 2022

AbstractBackgroundThe bimolecular fluorescence complementation (BiFC) assay has emerged as one of the most popular methods for analysing protein–protein interactions (PPIs) in plant biology. This includes its increasing use as a tool for dissecting the molecular mechanisms of chloroplast function. However, the construction of chloroplast fusion proteins for BiFC can be difficult, and the availability and selection of appropriate controls is not trivial. Furthermore, the challenges of performing BiFC in restricted cellular compartments has not been specifically addressed.ResultsHere we describe the development of a flexible modular cloning-based toolkit for BiFC (MoBiFC) and proximity labelling in the chloroplast and other cellular compartments using synthetic biology principles. We used pairs of chloroplast proteins previously shown to interact (HSP21/HSP21 and HSP21/PTAC5) and a negative control (HSP21/ΔPTAC5) to develop standardised Goldengate-compatible modules for the assembly of protein fusions with fluorescent protein (FP) fragments for BiFC expressed from a single multigenic T-DNA. Using synthetic biology principles and transient expression inNicotiana benthamiana, we iteratively improved the approach by testing different FP fragments, promoters, reference FPs for ratiometric quantification, and cell types. A generic negative control (mCHERRY) was also tested, and modules for the identification of proximal proteins by Turbo-ID labelling were developed and validated.ConclusionsMoBiFC facilitates the cloning process for organelle-targeted proteins, allows robust ratiometric quantification, and makes available model positive and negative controls. Development of MoBiFC underlines how Goldengate cloning approaches accelerate the development and enrichment of new toolsets, and highlights several potential pitfalls in designing BiFC experiments including the choice of FP split, negative controls, cell type, and reference FP. We discuss how MoBiFC could be further improved and extended to other compartments of the plant cell and to high throughput cloning approaches.
Stand carbon storage and net primary production in China’s subtropical secondary forests are predicted to increase by 2060
Authors: Jin Jia , Xiang Wenhua , Zeng Yelin , Ouyang Shuai , Zhou Xiaolu , Hu Yanting , Zhao Zhonghui , Chen Liang , Lei Pifeng , Deng Xiangwen , Wang Hui , Liu Shirong , Peng Changhui 
Published: May 2022

Abstract
Background
Forest ecosystems play an important role in carbon sequestration, climate change mitigation, and achieving China's target to become carbon (C) neutral by 2060. However, changes in C storage and net primary production (NPP) in natural secondary forests stemming from tree growth and future climate change have not yet been investigated in subtropical areas in China. Here, we used data from 290 inventory plots in four secondary forests [evergreen broad-leaved forest (EBF), deciduous and evergreen broad-leaved mixed forest (DEF), deciduous broad-leaved forest (DBF), and coniferous and broad-leaved mixed forest (CDF)] at different restoration stages and run a hybrid model (TRIPLEX 1.6) to predict changes in stand carbon storage and NPP under two future climate change scenarios (RCP4.5 and RCP8.5).

Results
The runs of the hybrid model calibrated and validated by using the data from the inventory plots suggest significant increase in the carbon storage by 2060 under the current climate conditions, and even higher increase under the RCP4.5 and RCP8.5 climate change scenarios. In contrast to the carbon storage, the simulated EBF and DEF NPP declines slightly over the period from 2014 to 2060.

Conclusions
The obtained results lead to conclusion that proper management of China’s subtropical secondary forests could be considered as one of the steps towards achieving China’s target to become carbon neutral by 2060.
Advances in the application of low-intensity pulsed ultrasound to mesenchymal stem cells
Authors: Xia Peng , Shi Yi , Wang Xiaoju , Li Xueping 
Published: May 2022

AbstractMesenchymal stem cells (MSCs) are stem cells that exhibit self-renewal capacity and multi-directional differentiation potential. They can be extracted from the bone marrow and umbilical cord, as well as adipose, amnion, and other tissues. They are widely used in tissue engineering and are currently considered an important source of cells in the field of regenerative medicine. Since certain limitations, such as an insufficient cell source, mature differentiation, and low transplantation efficiency, are still associated with MSCs, researchers have currently focused on improving the efficacy of MSCs. Low-intensity pulsed ultrasound (LIPUS) has mechanical, cavitation, and thermal effects that can produce different biological effects on organs, tissues, and cells. It can be used for fracture treatment, cartilage repair, and stem cell applications. An in-depth study of the role and mechanism of action of LIPUS in MSC treatment would promote our understanding of LIPUS and promote research in this field. In this article, we have reviewed the progress in research on the use of LIPUS with various MSCs and comprehensively discussed the progress in the use of LIPUS for promoting the proliferation, differentiation, and migration of MSCs, as well as its future prospects.
Enhancing therapeutic efficacy of human adipose-derived stem cells by modulating photoreceptor expression for advanced wound healing
Authors: Lee Sang Ho , Kim Yu-Jin , Kim Yeong Hwan , Kim Han Young , Bhang Suk Ho 
Published: May 2022

Abstract
Background
Human adipose-derived stem cells (hADSCs) have been widely used for regenerative medicine because of their therapeutic efficacy and differentiation capacity. However, there are still limitations to use them intactly due to some difficulties such as poor cell engraftment and viability after cell transplantation. Therefore, techniques such as photobiomodulation (PBM) are required to overcome these limitations. This study probed improved preclinical efficacy of irradiated hADSCs and its underlying molecular mechanism.

Methods
hADSCs were irradiated with green organic light-emitting diodes (OLEDs). Treated cells were analyzed for mechanism identification and tissue regeneration ability verification. Expression levels of genes and proteins associated with photoreceptor, cell proliferation, migration, adhesion, and wound healing were evaluated by performing multiple assays and immunostaining. Excision wound models were employed to test in vivo therapeutic effects.

Results
In vitro assessments showed that Opsin3 (OPN3) and OPN4 are both expressed in hADSCs. However, only OPN4 was stimulated by green OLED irradiation. Cell proliferation, migration, adhesion, and growth factor expression in treated hADSCs were enhanced compared to control group. Conditioned medium containing paracrine factors secreted from irradiated hADSCs increased proliferation of human dermal fibroblasts and normal human epidermal keratinocytes. Irradiated hADSCs exerted better wound healing efficacy in vivo than hADSCs without OLED irradiation.

Conclusions
Our study introduces an intracellular mechanism of PBM in hADSCs. Our results revealed that photoreceptor OPN4 known to activate Gq-protein and consequently lead to reactive oxygen species production responded to OLED irradiation with a wavelength peak of 532 nm. In conclusion, green OLED irradiation can promote wound healing capability of hADSCs, suggesting that green OLED has potential preclinical applications.
The stem cell quiescence and niche signaling is disturbed in the hair follicle of the hairpoor mouse, an MUHH model mouse
Authors: Choi Keonwoo , Park Sang-Hee , Park Seo-Yeon , Yoon Sungjoo Kim 
Published: May 2022

Abstract
Background
Hair follicle stem cells (HFSC) play an essential role in the maintenance of hair homeostasis; during the hair cycle, HFSC remain quiescent for most of its duration. The hairpoor mouse (+ /HrHp), an animal model of Marie-Unna hypotrichosis (MUHH), overexpresses hairless in the bulge, inner root sheath, and outer root sheath of HF and shows the same phenotype as in MUHH patients manifesting sparse hair with progression to alopecia with age. The aim of this study was to gain an understanding of the hair cycle and the status of HFSC during the hair cycle of the hairpoor mouse in order to delineate the pathogenesis of MUHH.

Methods
H&E staining was performed in order to define the state of the hair follicle. FACS analysis and immunostaining were performed at the 1st and 2nd telogen stages for observation of the HFSC. A label retaining assay was performed to determine the quiescent state of hair follicles. qRT-PCR was performed to determine expression of factors involved in niche signaling and Wnt signaling.

Results
We observed a drastic decrease in the number of hair follicles after the 1st telogen, followed by an intensified disturbance in the hair cycle with shorter anagen as well as 2nd telogen in the hairpoor mouse. A dramatic reduction in the number of CD34 expressing bulges as well as cells was observed at the telogen of the HFs, with prominent high proliferation of bulge cells, suggesting the loss of HFSC quiescence in the hairpoor mouse. The increased cell proliferation in HF was reiterated following the synchronization of the hair cycle, leading to acceleration of HF cycling. Reduced expression of Fgf18 and Bmp6, the factors involved in HFSC quiescence, was observed in the HFSC niche of the hairpoor mouse. In addition, disturbed expression of Wnt signaling molecules including Wnt7b, Wnt10b, and Sfrp1 was observed, which induced the telogen-to-anagen transition of HFs in the hairpoor mouse.

Conclusions
These results indicate that the quiescent state of HFSC is not properly maintained in the hairpoor mouse, consequently leading HFs to the completely disarrayed hair cycle. These findings may provide an understanding of an underlying mechanism for development of alopecia with age in MUHH patients.
Albatross analytics a hands-on into practice: statistical and data science application
Authors: Caraka Rezzy Eko , Lee Youngjo , Han Jeongseop , Lee Hangbin , Noh Maengseok , Do Ha Il , Gio Prana Ugiana , Pardamean Bens 
Published: May 2022

AbstractAlbatross Analytics is a statistical and data science data processing platform that researchers can use in disciplines of various fields. Albatross Analytics makes it easy to implement fundamental analysis for various regressions with random model effects, including Hierarchical Generalized Linear Models (HGLMs), Double Hierarchical Generalized Linear Models (DHGLMs), Multivariate Double Hierarchical Generalized Linear Models (MDHGLMs), Survival Analysis, Frailty Models, Support Vector Machines (SVMs), and Hierarchical Likelihood Structural Equation Models (HSEMs). We provide 94 types of dataset examples.
Switching control of latex balloon expansion by using switching valve mediated with the Coanda effect
Authors: Keita Kaneko , Kenjiro Takemura 
Published: May 2022

AbstractSoft robots have advantages in terms of safety, softness, and compliance compared to traditional robotic systems. However, fluid-driven soft actuators, often employed in soft robots, require a corresponding number of bulky pressure supplies/valves to drive. Here, we consider a valve that can control the flow without mechanical moving parts for simplifying the driving system of soft actuators. We developed a system comprising a pump, a switching valve, and two latex balloons to demonstrate the feasibility of introducing a fluid valve into soft robotics. As the valve, which makes use of the Coanda effect, can switch the flow between two outlets when the pressure difference between the outlets is 3 kPa, we employed a latex balloon connected to each outlet. The system can control the expansion of each balloon by switching the flow from the pump. The experimental results proved that the system could actuate each balloon.
Novel exterior cover design for radiant heat resistance of firefighting robots in large-scale petrochemical complex fires
Authors: Fujita Jun , Tamura Yoshihiro , Amano Hisanori , Ohno Kazunori , Tadokoro Satoshi 
Published: May 2022

AbstractFires in petrochemical complexes are inaccessible because of the intense radiant heat from flames. Therefore, water cannon robots require radiant heat countermeasures to perform firefighting safely. Conventional radiant heat countermeasures employ a self-spraying method, wherein the water cannon robot requires a water tank of capacity 1.5 m3 (= 1500 L) to function for 7–8 min in an environment with a 20 kW/m2 radiation heat. However, the water cannon robot has size limitations because it is transported on one transport vehicle (10 t truck) to the site, and only a tank of capacity ~ 0.02 m3 can be installed on the robot. To overcome these drawbacks, this study proposes a method that utilizes a mountable radiant heat-resistant exterior cover that works with a small amount of water. The cover is made of radiant heat-shielding fireproof clothing with an aluminum coating that reflects 90% of the radiant heat on the surface and a mist nozzle that sprays water on the back surface. The remaining 10% is removed by the heat of vaporization of water sprayed on the back of the clothing and natural convection. The amount of water required for cooling was reduced to 1/80th of that compared to self-spraying because of the use of the developed cover. The proposed method of radiation reflection via vaporization and natural convection can be employed to protect firefighting robots.
Right pneumothorax with congenital pericardial defect showed right atrium mimicking bulla in surgery
Authors: Sugiura Yasoo , Hashizume Toshinori 
Published: May 2022

Abstract
Background
Congenital pericardial defect (CPD) is found incidentally in cases of pneumothorax. CPD is seen in left side rather than right side and it is not generally known among thoracic surgeons how the inside of the pericardial space can be seen from the thoracic cavity in cases of pericardial defect.

Case presentation
A 52-year-old man with dyspnea was referred to our hospital because of the diagnosis of right pneumothorax. Chest radiography showed a right lung collapse and a pneumopericardium on the left side. Despite insertion of a chest tube, air leakage prolonged, bullectomy at the apex of the right lung was performed under thoracoscopy. During surgery, thoracoscope showed that the right atrium seemed as if it had been a non-pedunculated bulla or cardiac cyst. Heart beating, continuity with the heart, and the absence of respiratory motion could distinguish the right atrium from a bulla, and pericardial defect was confirmed. Preoperatively, the patient had no cardiac symptoms related to the CPD, and therefore, it was determined that a procedure to close the CPD was not necessary. Any complication and recurrence did not occur 6 months after surgery.

Conclusions
Right pneumothorax with CPD showed right atrium mimicking bulla in surgery. It is important to consider correction of CPD if there are cardiac symptoms at the onset of pneumothorax, and not to misinterpret the right atrium as a bulla.
Investigating and defining outcomes of suprapatellar versus infrapatellar intramedullary nailing of tibial shaft fractures: a protocol for a pilot randomised controlled trial
Authors: Thwaites Simon , Thewlis Dominic , Hall Kelly , Rickman Mark 
Published: May 2022

Abstract
Background
Anterior knee pain is often reported following intramedullary nailing of tibial shaft fractures. The aetiology remains unclear, but the surgical approach may play an important role. To date, no biomechanically validated method exists to assess patient outcomes specific to anterior knee pain in this cohort. The central aims of this study are to (1) evaluate the feasibility of a full-scale randomised controlled trial (RCT) investigating the influence of surgical approach on intramedullary nailing of tibial shaft fractures (suprapatellar versus infrapatellar nailing), (2) explore differences in clinical outcomes between the approaches, and (3) explore the development of a biomechanically validated methodology for assessing post-operative anterior knee pain and knee function specific to intramedullary nailing of tibial shaft fractures.

Methods
This pilot study will follow a prospective randomised controlled design at the Royal Adelaide Hospital and The Queen Elizabeth Hospital (South Australia). This study aims to recruit 60 patients between 18 and 60 years old who will be randomly assigned to either the suprapatellar or infrapatellar approach following a decision for intramedullary surgical fixation by the treating surgeon. All nails in this study will be Stryker T2 Alpha nails. Patients will undergo standard radiograph, magnetic resonance imaging, and clinical assessments in-line with their standard operative care, and complete a number of patient-reported and performance-based outcome measures. Performance-based outcome measures will be assessed utilising three-dimensional motion capture techniques. Follow-up time points are 3, 6, 12, and 18 months. Feasibility outcomes include ability to meet enrolment and retention metrics, compliance with all questionnaires and assessment procedures, and the occurrence of any adverse events. The primary clinical outcome is the incidence of anterior knee pain at 12 months after surgery.

Discussion
This study will establish the feasibility and inform the design of a large-scale RCT. Evaluation of all clinical data and patient outcomes will lead to the development of a new tool for assessing patient outcomes in this cohort. Limitations of the study include an unpredictable enrolment rate and loss to follow-up, small sample size, and the unknown ability of three-dimensional motion analysis to pick up the effects of anterior knee pain after tibial nailing.

Trial registration
This trial was prospectively registered on the 7 February 2020 on ANZCTR, ACTRN12620000109909.