bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Comparison between Frozen Elephant Trunk Technique and Conventional Total Arch Replacement, a Single-center Study
Authors: Phothikun Amarit , Yoongtong Dit , Nawarawong Weerachai , Woragidpoonpol Surin 
Published: November 2022

Objective: The advantage of the frozen elephant trunk technique (FET) is treating the aortic pathology extending from the aortic arch to the descending aorta in a one-stage operation. This study aimed to determine the early and long-term outcomes of total arch replacement with the frozen elephant trunk technique compared to conventional total arch replacement (cTAR).
Materials and Methods: This study was a single-center, retrospective cohort study. Patients who underwent aortic arch replacement between January 2009 and December 2020 were enrolled and divided into two groups. 32 patients underwent the FET and 47 patients underwent cTAR. Outcomes and survival analysis were compared.
Results: The 5-year survival for FET and cTAR were 87.27% and 82.55% and the aortic-related mortality was not significantly different (HR 0.97, 95%CI 0.22-0.43). Aortic re-intervention was significantly lower in the FET group accounting for 3.57%, while there was 39.1% in the cTAR group. FET significantly reduced aortic re-intervention compared with cTAR (HR 0.09, 95%CI 0.01-0.81). There were no differences in the incidence of stroke between the two groups. However, FET patients had significantly increased risks of paraplegia by 21% when compared to cTAR (Risk difference +0.21, 95%CI 0.02-0.40)
Conclusions: The FET technique had comparable early outcomes in terms of mortality, renal function, postoperative bleeding, and recurrent laryngeal nerve injury. Even though FET had a significant risk of postoperative paraplegia, FET reduced the aortic re-intervention rate without increasing aortic-related death.
Effect of Smoking on the Red Blood Cell Count, Hemoglobin Concentration, Hematocrit and Red Cell Indices in Adult Male Smokers
Authors: Nargish Shaheen , Kabir Md Humayun , Akhter Khaleda , Nahid Khondakar Alwan , Hossain Mohammad Monir , Karmakar Pijush , Ahmed Mainuddin , Islam Md Zakirul 
Published: November 2022

Background- Cigarette smoking is one of the common addictions. The effects of cigarette smoking on health are serious and, in many cases, deadly.
Objectives: The present study was carried to observe the effect of smoking on Red Blood Cells (RBC) count, Hemoglobin (Hb) concentration, Hematocrit (HCT) and Red cell indices in adult male smokers and compare it with non-smokers to create awareness.
Materials and Methods: In this study 50 adult male smokers and 50 adult male non-smokers aged from 20-45 years were taken. The smokers were regularly smoking for at least one year. Fresh peripheral blood sample from subjects were collected and analyzed for Red blood cell count, Hemoglobin concentration, Hematocrit and Red cell indices by using Mythmic-22 automated 5-part Hematology Analyzer.
Result: In this study the values of RBCs count, Hb concentration and Hematocrit showed significant increase (p<0.05) in smokers as compared to non-smokers. The values of Red cell indices (MCV, MCH, MCHC) were statistically not significant between two groups.
Conclusion: This study concluded that smoking causes persistent state of hypoxia in the body due to smoke contents which cause increase in RBCs count, Hb concentration and Hematocrit.
EMCJ. January 2022; 7(1): 1-5
CADÊ O TROCO DA MINHA COMPRA: MARKETING ENGANOSO
Authors: BELO DE SOUZA SIMONE , CARDOSO MARQUES EMERSON 
Published: November 2022

O presente ensaio visa demonstrar a prática de supressão de unidade fracionada de moeda de menor valor (troco), indevidamente justificada pelas empresas pela falta destas em circulação no mercado, incorrendo em costume ilegal e danoso aos clientes, em nome da estratégia mercadológica, nomenclatura utilizada de forma enganosa, em seu dia a dia. Tem por escopo, ainda, o objetivo de alertar que tal prática, por parte das empresas, gera significativo prejuízo ao sistema econômico e financeiro do país, pois desequilibra o processo de distribuição de rendas e riquezas, vez que parte delas fica retida com quem não deveria ter tal contemplação, porquanto estes valores são, de fato e de direito, de seus consumidores. Por fim, a pesquisa trata sobre o marketing enganoso, que se exibe como sendo um “costume institucionalizado” no cotidiano dos estabelecimentos e que deve ser combatido pelos órgãos de fiscalização do consumidor, de maneira que haja pleno respeito aos seus clientes.
Palavras-Chave: Troco. Retenção. Consumidor. Empresa. Marketing enganoso.

Abstract: This essay aims to demonstrate the practice of suppressing fractional units of lesser value currency (change), unduly justified by companies due to their lack of circulation in the market, incurring in an illegal and harmful custom to customers, in the name of the marketing strategy, nomenclature used deceptively, in their daily lives. Its scope is also to warn that such practice, on the part of companies, generates significant damage to the country´s economic and financial system, as it unbalances the process of income and wealth distribution, since part of it is retained with those who do not should have such contemplation, because these values are, in fact and in law, of its consumers. Finally, the research deals with deceptive marketing, which is displayed as an “institutionalized custom” in the daily life of establishments and that must be fought by consumer inspection agencies, so that there is full respect for their customers.
Keywords: Thing. Retention. Consumer. Company. Deceptive marketing.
Kreolisasi Bahasa Indonesia dengan Bahasa Arab pada Komunikasi Santri Pondok Modern Rafah Bogor
Authors: Lukman Syah Burhan , Abdul Aziz Agus Umar , Mu’ti Abdul 
Published: November 2022

Abstract
An educational institution that implements a language system requires a support system from all lines to realize active communication. However, it is the background of the students that affects the type of language that will be studied so that a new language is formed as a result of marriage between the original language and the language studied. The purpose of this study was to determine the art of communication for the students of Pondok Rafah and to find out the structure of the mixed language of Indonesian and Arabic dialects. This research includes library research using qualitative research methods which refers to the book Qualitative and Inquiry Research Design by John W. Cresswell with purposive sampling data collection technique. From the results of this study, the students of Pondok Rafah came from various ethnic and cultural backgrounds and one national language, namely Indonesia. So, a new language was formed as a result of the marriage of the original language with the language he was learning due to the lack of native speakers in Pondok. For example: لَيْسَ ذلِكَ غَرْضِي (that's not what it means) The correct one in Arabic Fushah is لَيْسَ هذَا هُوَ الْمَقْصُوْدُ However, Rafah's students still know the original Arabic language even if fellow alumni are mixed dialects because they have become identities.
STRATEGI KOMUNIKASI PEMASARAN PRODUK LOKAL I-LED MELALUI SOSIAL MEDIA FACEBOOK DI DESA KLAYAN KECAMATAN GUNUNG JATI CIREBON
Authors: Septiani Dea Nita , Siswoyo Mukarto , Wihayati Welly 
Published: November 2022

Tujuan pada penelitian yang berjudul “Strategi Komunikasi Pemasaran Produk Lokal I-LED Melalui Sosial Media Facebook” dengan jenis penelitian yang digunakan adalah penelitian kualitatif serta menghasilkan data yang bersifat deskriptif. Teknik pengumpulan data menggunakan teknik Observasi, wawancara mendalam, studi kepustakaan, dan dokumentasi. Teknik pemeriksaan keabsahan data menggunakan triangulasi dan ketekunan pengamatan. Teknik analisis data terdiri dari reduksi data, penyajian data, dan menarik kesimpulan. Hasil penelitian, menghasilkan data sebagai berikut: 1) Strategi komunikasi pemasaran pada I-LED berjalan dengan optimal, aktif pada berbagai bauran pemasaran. Ada dua dimensi yang harus ditingkatkan lagi, yaitu periklanan khususnya lewat internet dan Promosi Penjualan terutama dalam mengelola media sosial dan online shop agar komunikasi pemasaran dapat berjalan dengan optimal, 2) Faktor pendukung serta Faktor penghambat, seperti seperti kualitas produk yang ditawarkan, pemilihan media sosial, skill pelayanan pendukung dapat menarik konsumen, sedangkan faktor penghambat, yakni kurangnya tenaga kerja, kurang aktif dalam kegiatan Periklanan, dan belum mengikuti kegiatan pameran penjualan produk, dan 3) Upaya yang dilakukan seperti penambahan tenaga kerja baru dengan mengajak orang-orang sekitar untuk bekerja sama, hal tersebut diharapkan dapat meningkatkan efektivitas dalam kegiatan produksi serta meningkatkan penjualan produk, mulai aktif dalam kegiatan Periklanan dalam berbagai hal baik dalam word of mouth maupun di internet agar produknya dapat dikenal oleh khalayak luas. Selain itu I-LED juga mulai bekerja sama dengan beberapa komunitas aquarium air laut dan tawar di Cirebon untuk mengadakan suatu pameran agar masyarakat dapat mengenal lebih dalam mengenai kegiatan hobi aquarium air laut dan tawar.Kata Kunci: Strategi, Komunikasi Pemasaran, Produk Lokal, Media Sosial, Facebook
EDUKASI DAN PEMBUATAN TAMAN OBAT KELUARGA UNTUK PENCEGAHAN DAN PEMELIHARAAN KESEHATAN MASYARAKAT
Authors: Suryani Suryani , Kasmawati Henny , Fitrawan La Ode Muh. , Hikmah Nurull , Sida Nurramadhani A , Ruslin Ruslin , Aswani Aswani 
Published: November 2022

Indonesian people have used herbal ingredients for health. However, there are differences in natural resources and knowledge of plant processing techniques in medicine which differ from region to region. This community service aims to increase public knowledge about the types of plants that can be cultivated in Family Medicine Garden and their health benefits. Educational activities and the establishment of a Family Medicine Garden (TOGA) were held at the UHO Faculty of Pharmacy drug park. The method of educational activities is in the form of lectures and question-and-answer interactions, followed by manufacturing and planting medicinal plants at Family Medicine Garden. Education is carried out by introducing the efficacy of 21 plants, the parts used and the benefits of Family Medicine Garden. The activity went well, and the community was enthusiastic about getting new knowledge about types of plants and how to make Family Medicine Garden.
Wound healing mechanism of antimicrobial peptide cathelicidin-DM
Authors: Wang Guixi , Chen Zhizhi , Tian Pan , Han Qinqin , Zhang Jinyang , Zhang A-Mei , Song Yuzhu 
Published: November 2022

Background and Purpose: Chronic wound infections and the development of antibiotic resistance are serious clinical problems that affect millions of people worldwide. Cathelicidin-DM, an antimicrobial peptide from Duttaphrynus melanostictus, has powerful antimicrobial activity and wound healing efficacy. So, it could be a potential candidate to address this problem. In this paper, we investigate the wound healing mechanism of cathelicidin-DM to establish a basis for preclinical studies of the drug.Experimental Approach: The effects of cathelicidin-DM on cell proliferation and migration, cytokines, and mitogen-activated protein kinase (MAPK) signaling pathways were examined. Then mice whole skin wound model was constructed to evaluate the wound healing activity of cathelicidin-DM, and further histological changes in the wounds were assessed by hematoxylin-eosin staining (H&amp;E) and immunohistochemical assays.Key Results: Cathelicidin-DM promotes the proliferation of HaCaT, HSF, and HUVEC cells in a concentration-dependent manner and the migration of HSF, HUVEC, and RAW.264.7 cells. Moreover,cathelicidin-DM can involve in wound healing through activation of the MAPK signaling pathway by upregulating phosphorylation of ERK, JNK, and P38. However, cathelicidin-DM didn’t affect the secretion of IL-6 and TNF-α. At the animal level, cathelicidin-DM accelerated skin wound healing and early debridement in mice as well as promoted re-epithelialization and granulation tissue formation, α-SMA expression, and collagen I deposition in mice.Conclusion and Implications: Our data suggest that cathelicidin-DM can be engaged in the healing of infected and non-infected wounds through multiple pathways, providing a new strategy for the treatment of infected chronic wounds.
Dual-mode electrochemiluminescence and electrochemical sensor for alpha-fetoprotein detection in human serum based on vertically ordered mesoporous silica films
Authors: Chen Haiyun , Huang Jie , Zhang Rongjing , Yan Fei 
Published: November 2022

In this study, we demonstrated the highly sensitive detection of alpha-fetoprotein (AFP) by electrochemiluminescence (ECL) and electrochemistry (EC) based on the gated transport of the bifunctional probe (tris(1,10-phenanthroline) ruthenium (II) chloride, Ru (phen)3Cl2) into the nanochannels of vertically ordered mesoporous silica films (VMSFs). Due to the negatively charged surface and ultrasmall pore size, VMSF displays a signal amplification effect on Ru (phen)3Cl2 and is suitable for the construction of sensors with excellent sensitivity. With the linkage of (3-glycidyloxypropyl) trimethoxysilane, the anti-AFP antibody could covalently bind to the external surface of VMSF, generating a highly specific recognized sensing interface toward AFP. When AFP is presented, the formed immunocomplex hinders the diffusion of Ru (phen)3Cl2 to the underlying electrode surface, resulting in a decreased ECL or EC response. The dual-mode detection of AFP is achieved with a relatively low limit of detection (0.56 fg/ml for ECL and 4.5 pg/ml for EC) and a wide linear range (10 fg/ml∼1 μg/ml for ECL and 10 pg/ml∼1 μg/ml for EC). Moreover, owing to the inherent anti-fouling property of VMSF, satisfactory results in the analysis of human serum were obtained, showing the great potential of the designed strategy in clinical diagnosis.
PERM1 regulates energy metabolism in the heart via ERRα/PGC−1α axis
Authors: Oka Shin-ichi , Sreedevi Karthi , Shankar Thirupura S. , Yedla Shreya , Arowa Sumaita , James Amina , Stone Kathryn G. , Olmos Katia , Sabry Amira D. , Horiuchi Amanda , Cawley Keiko M. , O’very Sean A. , Tong Mingming , Byun Jaemin , Xu Xiaoyong , Kashyap Sanchita , Mourad Youssef , Vehra Omair , Calder Dallen , Lunde Ty , Liu Tong , Li Hong , Mashchek J. Alan , Cox James , Saijoh Yukio , Drakos Stavros G. , Warren Junco S. 
Published: November 2022

AimsPERM1 is a striated muscle-specific regulator of mitochondrial bioenergetics. We previously demonstrated that PERM1 is downregulated in the failing heart and that PERM1 positively regulates metabolic genes known as targets of the transcription factor ERRα and its coactivator PGC-1α in cultured cardiomyocytes. The aims of this study were to determine the effect of loss of PERM1 on cardiac function and energetics using newly generated Perm1-knockout (Perm1–/–) mice and to investigate the molecular mechanisms of its transcriptional control.Methods and resultsEchocardiography showed that ejection fraction and fractional shortening were lower in Perm1–/– mice than in wild-type mice (both p &lt; 0.05), and the phosphocreatine-to-ATP ratio was decreased in Perm1–/– hearts (p &lt; 0.05), indicating reduced contractile function and energy reserves of the heart. Integrated proteomic and metabolomic analyses revealed downregulation of oxidative phosphorylation and upregulation of glycolysis and polyol pathways in Perm1–/– hearts. To examine whether PERM1 regulates energy metabolism through ERRα, we performed co-immunoprecipitation assays, which showed that PERM1 bound to ERRα in cardiomyocytes and the mouse heart. DNA binding and reporter gene assays showed that PERM1 was localized to and activated the ERR target promoters partially through ERRα. Mass spectrometry-based screening in cardiomyocytes identified BAG6 and KANK2 as potential PERM1’s binding partners in transcriptional regulation. Mammalian one-hybrid assay, in which PERM1 was fused to Gal4 DNA binding domain, showed that the recruitment of PERM1 to a gene promoter was sufficient to activate transcription, which was blunted by silencing of either PGC-1α, BAG6, or KANK2.ConclusionThis study demonstrates that PERM1 is an essential regulator of cardiac energetics and function and that PERM1 is a novel transcriptional coactivator in the ERRα/PGC-1α axis that functionally interacts with BAG6 and KANK2.
Helium isotopes in Plinian and inter-Plinian volcanic products of Vesuvius, Italy
Authors: Gherardi Fabrizio , Barsanti Mattia , Principe Claudia , Magro Gabriella 
Published: November 2022

We investigated helium isotopes on gas extracted by crushing from melt and fluid inclusions in minerals from Plinian and inter-Plinian tephra and lavas of Vesuvius, Italy. Erupted products of different ages were considered, from Avellino eruption (1995 BCE) to the last eruption of 1944, with special focus on the 79 AD Plinian eruption. He/He 4 3 ratios between 1.5 and 2.7 RA were measured, with the highest values associated with rocks representative of the roof and the walls of the magma chambers (cumulates). Lowest values occurred in sanidines representative of magma-skarn interfaces. Noteworthy, the highest measured values of the 79 AD pumices were comparable with both lavas and tephra emitted from flank vents and under open-conduit conditions during the Medieval Period and Present Period of Vesuvius activity, and present-day fumarolic discharges. He 3/He 4 values are buffered within an extended, deep-seated reservoir at about 10 km filled with magma rising from the mantle. A fact that might potentially limit the accuracy of future eruption forecasting through monitoring of He/He 4 3 changes in Vesuvius fumaroles. Ageing and interaction with crustal rocks emerged as possible mechanisms that lowered the He/He 4 3 ratio of the melt during its intra-crustal magma chambers stay, with highest values associated with more dynamic conditions.
Effects of semaglutide on vascular structure and proteomics in high-fat diet-induced obese mice
Authors: Yue Lin , Chen Shuchun , Ren Qingjuan , Niu Shu , Pan Xiaoyu , Chen Xing , Li Zelin , Chen Xiaoyi 
Published: November 2022

BackgroundObesity is a chronic metabolic disease caused by a combination of genetic and environmental factors. To determine whether semaglutide could improve aortic injury in obese C57BL/6J mice, and further explore its molecular mechanism of action using proteomics.Methods24 C57BL/6J male mice were randomly divided into normal diet group (NCD group), high-fat diet group (HFD group) and high-fat diet + semaglutide group (Sema group, semaglutide (30 nmol/kg/d) for 12 weeks). The serum samples were collected from mice to detect blood glucose, insulin and blood lipid concentrations. Aortic stiffness was detected by Doppler pulse wave velocity (PWV). Changes in vascular structure were detected by HE, masson, EVG staining and electron microscopy. The aorta-related protein expression profiles were detected by proteomic techniques, and proteins with potential molecular mechanisms were identified.ResultsSemaglutide could reduce body weight, the concentrations of blood glucose, total cholesterol (TC), triglycerides (TG), lipoprotein cholesterol (LDL-C), and reduce the aortic PWV and ameliorate vascular damage in obese mice. The results of proteomic analysis showed there were 537 up-regulated differentially expressed proteins (DEPs) and 322 down-regulated DEPs in NCD/HFD group, 251 up-regulated DEPs and 237 down-regulated proteins in HFD/Sema group. There were a total of 25 meaningful overlapping DEPs in the NCD/HFD and HFD/Sema groups. GO enrichment analysis of overlapping DEPs found that these differential proteins were mainly located in the signaling pathways of the extracellular matrix. The most obvious changes of extracellular matrix associated proteins in the three experimental groups were Coll5a1, Lama4, Sparc.ConclusionSemaglutide may protect vascular structure and improve endothelial permeability by reducing the levels of Coll5a1, Lama4, Sparc in extracellular matrix, so as to improve vascular function and achieve vascular protection.
A neutrophil extracellular traps-associated lncRNA signature predicts the clinical outcomes in patients with lung adenocarcinoma
Authors: Ding Wencong , Li Biyi , Zhang Yuan , He Liu , Su Junwei 
Published: November 2022

Backgrounds: Neutrophil extracellular traps (NETs) play an important role in the occurrence, metastasis, and immune escape of cancers. We aim to investigate Long non-coding RNAs (lncRNAs) that are correlated to NETs to find some potentially useful biomarkers for lung adenocarcinoma (LUAD), and to explore their correlations with immunotherapy and chemotherapy, as well as the tumor microenvironment.Methods: Based on the The Cancer Genome Atlas (TCGA) database, we identified the prognosis-related lncRNAs which are associated with NETs using cox regression. The patients were then separated into two clusters based on the expression of NETs-associated lncRNAs to perform tumor microenvironment analysis and immune-checkpoint analysis. Least absolute shrinkage and selection operator (LASSO) regression was then performed to establish a prognostic signature. Furthermore, nomogram analysis, tumor mutation burden analysis, immune infiltration analysis, as well as drug sensitivity analysis were performed to test the signature.Results: Using univariate cox regression, we found 10 NETs-associated lncRNAs that are associated with the outcomes of LUAD patients. Also, further analysis which separated the patients into 2 clusters showed that the 10 lncRNAs had significant correlations with the tumor microenvironment. Using LASSO regression, we finally constructed a signature to predict the outcomes of the patients based on 4 NETs-associated lncRNAs. The 4 NETs-associated lncRNAs were namely SIRLNT, AL365181.3, FAM83A-AS1, and AJ003147.2. Using Kaplan-Meier (K-M) analysis, we found that the risk model was strongly associated with the survival outcomes of the patients both in the training group and in the validation group 1 and 2 (p &lt; 0.001, p = 0.026, and p &lt; 0.01). Using receiver operating characteristic (ROC) curve, we tested the sensitivity combined with the specificity of the model and found that the risk model had a satisfactory level of 1-year, 3-year, and 5-year concordance index (C-index) (C = 0.661 in the training group, C = 0.679 in validation group 1, C = 0.692 in validation group 2). We also explored the immune microenvironment and immune checkpoint correlation of the risk model and found some significant results.Conclusion: We constructed a NETs-associated lncRNA signature to predict the outcome of patients with LUAD, which is associated with immunephenoscores and immune checkpoint-gene expression.