bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors Determining User Satisfaction with Counter Services of Local Government Offices: On-Site Survey at Higashihiroshima City Hall, Japan
Authors: Moteki Yasutoshi 
Published: April 2022

Abstract
This study empirically investigated the major factors determining customer satisfaction with counter services in local government offices in Japan, comparing them with the results of similar surveys conducted in 2020. It considers visitors’ direct experience of counter services through an on-site survey conducted at a municipal government building, including (1) customer satisfaction research in various countries, especially the expectancy disconfirmation model; (2) the SERVQUAL model; and (3) subsequent methods emphasising customer’s direct experience. SERVQUAL is a five-group scale for evaluating service quality with respect to customer satisfaction. However, in actual organisational settings, five groups of questions may be burdensome to respondents and may reduce the collection rate. This research involves conducting on-site surveys on customer satisfaction or service quality of the local government office using an original three-group questionnaire scale and tailoring the question items to a scale for Japanese local administration settings. A total of 1,000 questionnaires were distributed over four weekdays at the City Hall. As of September 27, 2021, there were 537 respondents (response rate: 53.7%). Regression analysis indicated that the independent variables of group C (service delivery) were most influential, followed by those of groups B (human-related factors) and A (office hardware) (adjusted R2 value: .58). This is consistent with the results of a previous survey of users of Osaka City Government’s ward offices. Focusing on the second principal component of the service delivery category (C), promptness and short waiting times do not necessarily affect customer satisfaction positively. The author's scale, which is a 3-group scale, is less than SERVQUAL's 5-group scale. The validation of a simple three-group scale for public facility user satisfaction provides useful insights for local government practitioners seeking to conduct similar satisfaction surveys and offers methodological suggestions for researchers examining service satisfaction in public and private service organizations.
Lithospheric warping underlies the formation of back-arc basins
Authors: Orvos Peter 
Published: April 2022

Abstract
Back-arc basins represent intriguing phenomenon of the lithospheric evolution. They are the places of potential subduction initiation, what makes them highly important features within the theory of plate tectonics. The circumstances of their origin and life cycle are not well understood and whether the retreat of subduction is a cause or consequence of back-arc basin development remains an open issue. In the presented work, the new approach has been used, based on the model of thin shell warping. Within this concept, the plate warps due to proximity of translational boundary when its forward movement is constrained. During the process, the lithospheric slab can steepen and sink to the 660 km transition layer, reaching an amphitheatre-like geometry. The specific shape of deformation resembles the topology of several reference basins from the Western Pacific region. Results show that subduction retreat, back-arc extension and arcuate geometry may represent only different demonstrations of one underlying physical mechanism. Modelling suggests that the movement of plates and their interactions, together with the curvature of the Earth’s surface, could be responsible for the formation of back-arc basins.
Human basic Fibroblastic Growth Factor production by codon optimization in rice cell suspension culture
Authors: bastami Meysam , Hosseini Ramin 
Published: April 2022

Abstract
Human basic fibroblastic growth factor (hbFGF), possesses a wide range of highly important biological functions, including cell proliferation, protein synthesis and metabolism. This study is the first investigation to transfer a partly home-made hbFGF gene cassette into rice cells. The human bFGF coding sequence was optimized for expression in rice in order to improve its in vitro production. Following synthesis, the expression cassette was constructed by fusing the PCR- amplified Ram3D promoter and terminator sequences in two separate steps to the optimized hbFGF CDS. Then, the cassette was transferred into the pCAMBIA1304 shuttle vector. This vector also contained a signal peptide for the secretion of the recombinant protein into the culture medium. Rice (Oryza sativa cv. Kanto 51) seeds were cultured on the 2N6PG medium composing of the N6 basal medium supplemented with 2 mg/L 2,4-D for callus induction. Callus transformation was performed by employing Agrobacterium tumefaciens strain LBA 4404. The integration of bFGF gene into the chromosomes of transgenic calli was verified by genomic DNA PCR. A transformed callus line expressing the highest level of bFGF (38.1 mg/kg FW) was recognized by ELISA and selected for the establishment of cell suspension culture. The developed transgenic rice cell culture was able to express and secrete the recombinant bFGF into the culture medium on day 3 after incubation of cells in the sucrose-free medium. The SDS-PAGE and Western blot analyses detected the bFGF in the culture medium with a molecular weight of ~17 kDa under reducing conditions. The purification of bFGF was performed using HiTrap Heparin HP column. Interestingly, the activity assay indicated that the rice derived bFGF stimulated the proliferation of NIH/3T3 cells similar to the commercial bFGF.
Safety Assessment and Prophylactic Efficacy of Moringa stenopetala Leaf Extract through Mitigation of Oxidative Stress in BV-2 Microglial Cell
Authors: Adefegha Stephen Adeniyi , Mostardeiro Vitor , Morsch Vera Maria , Morel Ademir F. , Cruz Ivana Beatrice Manica , Somacal Sabrina , Schetinger Maria Rosa Chitolina 
Published: April 2022

Abstract
Moringa stenopetala is often consumed as food and used in folkloric medicine for the management of several diseases. Purpose: This study was set up in order to assess the effect of aqueous extract of Moringa stenopetala on cell viability and oxidative stress biomarkers in BV-2 microglial cells. Aqueous extracts of M. stenopetala were prepared, lyophilized and reconstituted in 0.5% dimethylsulphoxide (DMSO). Cells were treated with M. stenopetala extracts (0.1 - 100 µg/ml) for cell viability and nitric oxide (NO) production tests. However, M. stenopetala extract (50 µg/ml) was used in the treatment of cells for the determination of protein carbonyl content and reactive oxygen species (ROS) level. Incubation of BV-2 microglia cell with M. stenopetala extract maintained cell viability, diminished NO and ROS levels, and reduced protein carbonyl contents Chlorogenic acid, rutin, kaempferol and quercetin derivatives were the main phenolic compounds identified in M. stenopetala leaf extract. These phenolic compounds present in M. stenopetala may be responsible for the mitigation of oxidative stress in BV-2 microglial cells.
Effects of a safe sex intervention on the commercial sexual behaviour of brothel sex workers in Bangladesh: Evidence from a randomized controlled trial
Authors: Hasnain Md , Islam Asad , Smyth Russell , Tan HongQi Alexis , Wang Liang Choon 
Published: April 2022

Abstract
Objectives. To evaluate the effect of the intervention, consisting of a combination of having information about sexually transmitted infections, especially human immune deficiency virus, and receiving training in negotiation to increase safer sexual behaviours among female sex workers and their clients. Methods. We implemented a randomized controlled study which recruited 1,332 female sex workers from eight different brothels in Bangladesh. The study consisted of a control and two treatments (T1 and T2). Participants in T1 took part in a safe sex information session and participants in T2 participated in a safe sex information session and received a counselling session in which they were taught the skills and techniques to negotiate with their clients to get them to wear condoms. Results. In this study we found no improvement in the sexual behaviour of female sex workers upon intervention. we find minimal differences in treatment effects between the control and treated participants. We did, however, find that participants in T2 were marginally more likely to engage in protected sexual transactions than T1 participants.Conclusions. Although the implemented intervention did not work, in this study, we examined several factors that might explain this result and help to develop future effective interventions.
The vaginal outlet: Anatomy and histology discoveries
Authors: Ostrzenski Adam 
Published: April 2022

Abstract
Purpose
The vaginal outlet (VO) anatomy and histology have not been described in scientific-clinical literature or traditional anatomy textbooks. The question of the present study is whether the VO has a different anatomical structure(s) and histology than the vaginal wall? The objectives are to describe VO’s gross and topographic anatomy, histology stained with hematoxylin and eosin, and document findings by digital photograms.
Methods
Ten fresh human adult female corpses were subjected to stratum-by-stratum anatomical, macro, and micro dissections in sagittal, transverse, and coronal planes. The multiple excisional biopsies were obtained from VO at 12, 3, 6, and 9 o’clock locations and submitted to histological examination.
Results
VO consisted of specific anatomical structures in vertical orientation and fused with horizontally located the vaginal wall at the hymeneal plate level. VO had three identifiable anatomical-histological layers a) the hymeneal membrane, b) the hymeneal ring, c) the hymeneal plate, and had specific histological characteristics features that distinguish VO from the vaginal wall. In addition, the vaginal wall was connected to the vaginal outlet at the level of the hymenal plate, the deepest layer of the vaginal outlet.
Conclusions
The vaginal outlet is an anatomical structure consisting of the hymeneal membrane, hymeneal ring, hymeneal plate, and it is in vertical topographic orientation. Anatomically and histologically, it differs from the vaginal walls, and the vaginal wall fuses with the hymeneal plate.
Risk factors associated with chemotherapy-induced nausea and vomiting among women receiving highly emetogenic chemotherapy: individual patient-based analysis of three prospective antiemetic trials
Authors: Yeo Winnie , Ngai Nicole TY , Yip Christopher CH , Mo Frankie KF , Yeo Victoria A , Ko Jonathan WH , Li Leung , Lau Thomas KH , Lai Kwai T , Pang Elizabeth , Yip Claudia CH , Kwok Carol CH , Molassiotis Alex 
Published: April 2022

Abstract
Purpose: Although risk factors related to chemotherapy-induced nausea and vomiting (CINV) have been identified by prior studies, there are only few studies evaluating risk factors associated with the contemporary antiemetic prophylaxis, including that of olanzapine/aprepitant- or NEPA- containing regimens. The present study aimed to identify the risk factors related to CINV development in Chinese breast cancer patients receiving doxorubicin and cyclophosphamide chemotherapy.Methods: Data from 304 patients who were enrolled to 3 previously reported prospective antiemetic studies were included. Multivariate logistic regression models were used to predict risk factors associated with the occurrence of CINV. Additionally, likelihood of treatment failure in relation to number of risk factors of individual patient was evaluated.Results: Multivariate analysis of the entire study group revealed that obese status (defined as body mass index >/= 25.0 kg/m2) and the use of olanzapine/aprepitant- or NEPA- containing antiemetic regimens were associated with high likelihood, while history of motion sickness was associated with lower likelihood, of complete response (CR) and ‘no nausea’ in the overall phase. History of vomiting in pregnancy was also associated with lower likelihood of overall CR. Patients with increasing number of risk factors had higher likelihood of treatment failure as well as shorter time to first vomiting. Those who did not achieve CR and ‘no nausea’ in the first cycle were less likely to achieve these parameters in the subsequent cycle of chemotherapy.Conclusions: This present study confirmed the previously reported risk factors to be important for CINV in Chinese breast cancer patients receiving doxorubicin and cyclophosphamide. Further optimization of CINV control is required for patients with identifiable risk factors; olanzapine/aprepitant- or NEPA- containing prophylaxes are the preferred contemporary antiemetics regimens for Chinese breast cancer patients undergoing doxorubicin and cyclophosphamide chemotherapy.
A person-centred intervention remotely targeting family caregivers’ support needs in the context of allogeneic hematopoietic stem cell transplantation - a feasibility study
Authors: Kisch Annika M , Bergkvist Karin , Adalsteinsdóttir Sólveig , Wendt Christel , Alvariza Anette , Winterling Jeanette 
Published: April 2022

Abstract
Purpose Allogeneic hematopoietic stem cell transplantation (HSCT) is an intensive curative treatment that increases family caregivers’ burden. The aim of this study was to explore the feasibility of remotely assessing and addressing family caregivers’ support needs in terms of demand and acceptability using the Carer Support Needs Assessment Tool Intervention (CSNAT-I) in the HSCT context.Methods CSNAT-I consists of an evidence-based tool and a five-stage person-centred process. The intervention was performed remotely by two designated nurses from two HSCT centres, one before HSCT and the second 6 weeks after (November 2020 to March 2021). To capture the experiences of using CSNAT-I, interviews were conducted with family caregivers and reflections were gathered from the designated nurses.Results Of 34 eligible family caregivers, 27 participated, 70% were partners and the rest children, siblings, or other relatives. The main support needs were: Knowing what to expect in the future and Dealing with your feelings and worries. The most frequent support actions according to CSNAT-I were psychological support and medical information. Four categories summarised family caregivers and designated nurses’ experiences: CSNAT-I was relevant and became an eye opener; Nurses’ experiences were important for enabling trustful CSNAT-I conversations; CSNAT-I provided family caregivers with support and a sense of security; and CSNAT-I gave family caregivers insight and enabled change. Conclusion Both family caregivers and designated nurses experienced that using CSNAT-I in an HSCT context was feasible and had the potential to provide valuable support for most of the participating family caregivers.
Heavy metals pollution in leachates and its impacts on quality of groundwater around Iringa municipal solid waste dumpsite.
Authors: Sanga Victor Fanuel , Pius Christina Fabian , Kimbokota Fikira 
Published: April 2022

Abstract
This paper presents the results on the heavy metals concentrations in leachates and on the quality of groundwater resources as impacted by leachates leaking from Iringa municipal solid waste dumpsite. The results on physicochemical properties such as pH, electrical conductivity (EC), and total dissolved solids (TDS) that were analyzed by using an electronic multiparameter analyser in both leachates and groundwater samples are also presented. Heavy metals (Fe, Pb, Cd, Cr, Cu, Ni, Mn, and Zn) were quantified using Atomic Absorption Spectrophotometer (AAS). Relatively higher electrical conductivity which ranged from 14090 ± 62.34 to 26700 ± 104.10 µS/cm and total dissolved solids (76930 ± 41.39–13360 ± 59.40 mg/L) than FAO irrigation water standard were recorded in all leachates. All leachates were identified to be alkaline in nature with pH ranging from 7.40 ± 0.02 to 9.10 ± 0.01; this signified older age of the Iringa municipal dumpsite which is at its methanogenic phase. Heavy metals analysis indicated no potential threat of Cd as its concentrations in all leachates were below the detection limit (< 0.01 mg/L). Meanwhile, the concentrations of Fe, Pb, Cr, Cu, Ni, Mn, and, Zn in all leachate samples were either above TBS guideline limits for wastewater reuse, or FAO standards for irrigation water. In all groundwater samples, total dissolved solids (446 ± 55.30–776 ± 52.34 mg/L) and pH (7.15 ± 0.02–7.6 ± 0.03) were within WHO acceptable range (pH = 6.5–8.5) for drinking water. Electrical conductivity (869 ± 89.64 to 1570 ± 105.50 µS/cm values) in three groundwater samples was above the WHO's desirable limit for drinking water. Whereas in some groundwater samples, the concentrations of Fe, Pb, Mn, and Zn exceeded WHO guideline limits for drinking water quality, implying that the water is unsuitable for human consumption without treatment. The results of Principal Components Analysis (PCA) in groundwater samples indicated that both geologic and anthropogenic processes could be the possible source of heavy metals pollution in the groundwater. However, the presence of heavy metals can be used as tracers of groundwater contamination in the study area. Moreover, water quality indices (WQI) for all groundwater samples ranged from 169.07 to 220.72 translating from poor quality (≤ 100 WQI ≤ 199.99) to very poor quality ((≤ 200 WQI ≤ 299.99). Therefore, the present study calls for the proper management of solid waste in the Iringa municipality to reduce further pollution of groundwater reservoirs through leachate percolation.
Malnutrition Screening and Treatment in Pediatric Oncology: A systematic review
Authors: Franke Jessica , Bishop Chris , Runco Daniel V. 
Published: April 2022

Abstract
Purpose
Malnutrition and cachexia during pediatric cancer treatment worsen toxicity and quality-of-life. Clinical practice varies with lack of standard malnutrition definition and nutrition interventions. This systematic review highlights available malnutrition screening and intervention data in childhood cancer and the need for standardizing assessment and treatment.
Methods
Ovid Medline, CINAHL, and Cochrane Library were searched for studies containing malnutrition as the primary outcome with anthropometric, radiographic, or biochemical measurements. Secondary outcomes included validated nutritional assessment or screening tools. Two authors reviewed full manuscripts for inclusion. Narrative analysis was chosen over statistical analysis due to study heterogeneity.
Results
The search yielded 234 articles and 17 articles identified from external sources. Nine met inclusion criteria with six nutritional intervention studies (examining appetite stimulants, nutrition supplementation, and proactive feeding tubes) and three nutritional screening studies (algorithms or nutrition support teams) each with variable measures and outcomes. Both laboratory evaluations (albumin, prealbumin, total protein) and body measurement (weight loss, mid-upper arm circumference) were used. Studies demonstrated improved weight, without difference between formula or appetite stimulant used. Screening studies yielded mixed results on preventing weight loss, weight gain, and survival.
Conclusion
Our review demonstrated a paucity of evidence for malnutrition screening and intervention in pediatric cancer treatment. While a variety of malnutrition outcomes, interventions, and screening tools exist, nutritional interventions increased weight and decreased complications. Screening tools decreased malnutrition risk and may improve weight gain. Potential age- and disease-specific nutritional benefits and toxicities also exist, further highlighting the benefit of standardizing malnutrition definitions, screening, and interventions.
Oligo replication advantage driven by GC content and Gibbs free energy
Authors: Qi Hao , Qiao Hongyan , Gao Yanmin , Wei Yanan , Li Jiaojiao , Liu Qian , Wang Zhaoguan 
Published: April 2022

Abstract
Large scale DNA oligo pools are emerging as a novel material in a variety of advanced applications. However, GC content and length cause significant bias in amplification of oligos. We systematically explored the amplification of one oligo pool comprising of over ten thousand distinct strands with moderate GC content in the range of 35–65%. Uniqual amplification of oligos result to the increased Gini index of the oligo distribution while a few oligos greatly increased their proportion after 60 cycles of PCR. However, the significantly enriched oligos all have relatively high GC content. Further thermodynamic analysis demonstrated that a high value of both GC content and Gibbs free energy could improve the replication of specific oligos during biased amplification. Therefore, this double-G (GC content and Gibbs free energy) driven replication advantage can be used as a guiding principle for the sequence design for a variety of applications, particularly for data storage.
Premature termination codon rs67047829 in ERV3-1 may be protective against obesity
Authors: Clark Jeremy S.C. , Podsiadło Konrad , Sobalska-Kwapis Marta , Marciniak Błażej , Rydzewska Kamila , Ciechanowicz Andrzej , Wetering Thierry , Strapagiel Dominik 
Published: April 2022

Abstract
Premature termination codons are usually single nucleotide polymorphisms which can have high, but ethnically-dependent, prevalence in a population. The aim of this fishing study was to assess all such premature termination codons (n=140) from a database (with 551915 exonic variants; HumanCoreExome-24 v1.0 and v1.1 BeadChip) with association with obesity in a large sample (n=5757) of the Polish population. Statistical analyses were conducted using regression models with body mass index (BMI) as a continuous variable. Interactions with year of birth and sex; and recessive, dominant and heterozygote models for males and females together and then separately were assessed. A significant association, which was interaction independent, was found between the ERV3-1: rs67047829 heterozygote model and BMI with both sexes together (p-value adjusted for year of birth and sex: 0.001; BMI for AA, GA, GG: means (standard deviations): 22.9 (3.68), 24.7 (4.44), 24.9 (4.59)). Corresponding values for males, but not for females, were also significant: (p=0.003; AA 22.2 (3.96), GA 23.8 (4.44), GG 24.3 (4.96)). As rs67047829 produces a stop codon in the Endogenous retrovirus group 3 member 1, envelope protein, the mRNA of which is highly expressed in adipose tissue, it is possible this premature termination codon provides protective effect against obesity.