bluuebunny/crossref_metadata_2025_split huggingface dataset:
NOTCH2NLC GCC repeats are not expanded in Italian Amyotrophic Lateral Sclerosis patients
Authors: Manini Arianna , Gagliardi Delia , Meneri Megi , Antognozzi Sara , Bo Roberto Del , Comi Giacomo Pietro , Corti Stefania , Ronchi Dario 
Published: March 2022

Abstract
IntroductionRepeat expansions in genes other than C9orf72 and ATXN2 have been recently associated with Amyotrophic Lateral Sclerosis (ALS). Indeed, an abnormal number of GGC repeats in NOTCH2NLC has been recently reported in 0.7% of sporadic ALS patients from mainland China. This finding was not confirmed in an ALS cohort of subjects from Taiwan. As the involvement of expanded NOTCH2NLC alleles in ALS is debated, we addressed this point by evaluating NOTCH2NLC repeat expansions in an Italian cohort of ALS patients.MethodsA screening analysis of NOTCH2NLC GGC repeats was performed by repeat-primed polymerase chain reaction (RP-PCR) in a cohort of 350 probable/definite ALS Italian patients.ResultsMean age at onset was 60.7 years (SD 13.6), and 62.6% were males. Sporadic cases were 329 (94.0%), and most patients had a spinal onset (71.8%). None of our patients showed the typical sawtooth tail pattern on RP-PCR, thus excluding abnormal repeat expansion in NOTCH2NLC.DiscussionOverall, we suggest that NOTCH2NLC expanded alleles might be absent or at least extremely rare in ALS Italian patients. Further investigations in larger cohorts with different ethnic backgrounds are required to support the involvement of NOTCH2NLC in ALS.
Effects of in vitro storage temperature and duration time on the state and function of primary granulosa cells after isolated from human follicular fluid
Authors: Zhang Tongwei , Fan Huiying , Zhang Junya , Hu Jingyi , Yang Guang , He Qina , Bai Yucheng , He Jiahuan , Xu Ziwen , Yao Guidong 
Published: March 2022

Abstract
From the collection of human follicular fluid to the isolation of primary granulosa cells, the duration of these two time points is variable. And there is no specific experiment to explore which temperature will make the cell state better in subsequent experiments. In this study, the collected follicular fluid was divided into 7 groups: one fresh control group, three in vitro storage temperatures (4℃, Room Temperature, and 37℃) and two storage duration times (4h and 8h). After isolating the cells, they were cultured in vitro to evaluate their state and function. The results showed that granulosa cells of the fresh control group had better cell viability and steroid hormone secretion ability than the other treatment groups; with the prolongation of in vitro storage time, the cell viability and steroid hormone secretion ability have decreased to varying degrees; low-temperature in vitro storage is conducive to the maintenance of cell survival and steroid hormone secretion ability, which is also confirmed by the detection of ROS, which can reflect the oxidative stress state of cells. This study showed that after obtaining follicular fluid, the in vitro storage time should be shortened as much as possible, and stored at low temperature, which can keep cells maintain good activity and steroid hormone secretion ability, and reduce oxidative stress and aging. It is of great significance for improving the state of human primary granulosa cells after isolation and subsequent experiment, and it also has certain guiding significance for standardization experimental research programs.
Narrative Elaboration Makes Misinformation and Corrective Information Regarding COVID-19 More Believable
Authors: Greer Joanna , Fitzgerald Kaitlyn , Vijaykumar Santosh 
Published: March 2022

Abstract
Objective
People gather information about health topics from online channels oftentimes awash with misinformation. Investigating this problem during the COVID-19 pandemic is important, as the misinformation effect occurs when misleading details are embedded in narratives and questions. This pilot study investigated whether narrative elaboration increases believability in misinformation statements about COVID-19, and willingness to share these statements online.
Results
Results from our online survey (n = 80) demonstrated that narrative elaboration increased believability in both misinformation and accurate statements, with a more pronounced effect on younger adults. Future research may investigate cognitive vulnerabilities imposed by elaborate narratives embedded in online health misinformation with increased attention on developing misinformation resilience among younger adults.
C3aR costimulation enhances antitumor efficacy of CAR-T through promoting Th17 expansion and memory T phenotype
Authors: Lai Peilong , Chen Xiaomei , Wang Yulian , Wang Jinghua , Zhang Yuchen , Geng Suxia , Li Peng , Du Xin , Weng Jianyu , Pei Duanqing 
Published: March 2022

Abstract
Chimeric antigen receptor (CAR)-modified adoptive T-cell therapy is a promising immunotherapy for hematologic malignancies. However, the efficacy in extramedullary leukemia is still limited and new strategies with improved anti-tumor activity are highly needed. Since C3aR activation builds a bridge between innate immune and adaptive immune and it could trigger Th17 response, we hypothesized that C3aR incorporation as costimulatory domain would augment anti-tumor activity of CAR-T. To address this possibility, we introduced a genetically engineered construct, comprising the domain of C3aR into CAR-T cells. The generated BB-ζ-C3aR CAR-T exhibited potent cytolytic ability to eradicate various tumor cells expressing CD19 or BCMA in vitro. When administrated intravenously into the xenografts leukemia NCG mice who received CD19 + or BCMA + expressing tumor cells, BB-ζ-C3aR CAR-T cells reduced the tumor burden and improved the survival of mice. Of note, these BB-ζ-C3aR CAR-T cells could effectively eradicate subcutaneous CD19 + tumor cells, highlighting the potential therapy for extramedullary leukemia. Mechanistically, the BB-ζ-C3aR CAR-T cells preferred to exhibit tumor-killing Th17 phenotype and suppressed the tumor-tolerated Treg function. In addition, the induction of memory T cells phenotype in BB-ζ-C3aR CAR-T group indicated their long duration effects. Taken together, our findings suggest that the application of C3aR costimulation to boost CAR-T-cell activity is efficacious against aggressive tumor cells via Th17 expansion and memory T cell induction.
Role of the elastography strain ratio using transrectal ultrasonography in the diagnosis of prostate cancer and clinically significant prostate cancer
Authors: Yoo Jeong Woo , Koo Kyo Chul , Chung Byung Ha , Lee Kwang Suk 
Published: March 2022

Abstract
INTRODUCTIONThis study investigated the efficacy of the elastography strain ratio (ESR) as a predictor of prostate cancer (PCa) in targeted prostate biopsy.METHODSIn total, 257 patients who underwent magnetic resonance imaging-targeted biopsy were enrolled. Before biopsy, we placed regions of interest (zone A and B) in the lesion and levator ani. The ESR was measured as zone A/zone B. Multivariate analyses were performed to predict PCa and clinically significant PCa.RESULTSThere were 206 (71.5%) positive cancer lesions. No difference in digit rectal examination findings was found between patients with and without PCa. For predicting clinically significant PCa, an ESR ≥ 6.8 was significantly higher in the PCa (+) group than in the PCa (−) group (p < 0.001). The area under the receiver operating characteristic curve (AUC) for the conventional variables (model 1) plus the ESR was 0.845, which was significantly higher than that for model 1 (p = 0.001). In prostate imaging reporting and data system score 3 lesions, an ESR ≥ 4.6 was a significant predictor of PCa (p = 0.002). The AUC in model 1 plus the ESR was 0.856, which was significantly higher than that in model 1 alone (p = 0.017).CONCLUSIONSThe ESR is useful for predicting clinically significant PCa.
Earthquake sedimentary event record in Early Triassic in southeastern Sichuan Basin, SW China
Authors: Zeng Jianli , Zhang Xi , Zhang Tingshan 
Published: March 2022

Abstract
There are some macroscopic and microscopic sedimentary structures of seismic events in the outcrops in Lower Triassic Feixianguan Fm (T1f). and Jialingjiang Fm (T1j). in southeastern parts of Sichuan Basin. The following basic types of sedimentary structures could be observed: Soft-sediment liquefied deformation structures include curled deformation (sliding deformation structure," sausage structure") and microscopic folds (laminated plastic deformation). Seismites emerge in T1f1 and T1f2 in Baimiaozi section, Beibei, Chongqing, while in T1j2 at Longdongwan, Xuyong, Sichuan, as well as the same strata at Xingwen, Sichuan and Xishui, Guizhou. The sedimentary sequences of seismites in outcrops are clear, presenting the whole process of paleoearthquake from strong to weak. The author argues that the Sanjiang tectonic belt represented by the evolution of Yidun arc in the western Sichuan had strong activities at the west margin of the upper Yangtze plate, which may be related to the formation of seismites in the early Triassic. Earthquake activities and volcanic eruption can be triggered in large areas by the plate collision and the subduction of oceanic crust in the Sanjiang tectonic belt.
Ruptured Basilar Artery Perforator Aneurysm Definitely Diagnosed With Intraoperative Microsurgical Findings: Case Report and Literature Review
Authors: Kumagawa Takahiro , Otani Naoki , Kakei Yuzo , Otaki Ryo , Negishi Hiroshi , Suma Takeshi , Yoshino Atsuo 
Published: March 2022

Abstract
Background: Initial three-dimensional computed tomography angiography and cerebral angiography fail to identify any aneurysm in 20% of cases of subarachnoid hemorrhage. Basilar artery (BA) perforator aneurysm is rare and about 30–60% were not identified by initial angiography.Case presentation: A 71-year-old male was transferred with sudden onset of headache and loss of consciousness. Computed tomography demonstrated subarachnoid hemorrhage, but no ruptured aneurysm was detected. Repeat preoperative cerebral angiography indicated bifurcation aneurysm of the circumflex branch of the superior cerebellar artery perforator but microsurgical observation identified BA perforator aneurysm.Conclusions: If the location of the BA perforator aneurysm cannot be clearly identified, as in this case, repeat angiography should be considered, and the treatment strategy should be decided based on detailed consideration of the site of the aneurysm.
Is it feasible to measure intra-abdominal pressure using a balloon-tipped rectal catheter? – Results of a pilot study
Authors: Malbrain Manu L.N.G. , Staelens Anneleen S , Heymans Ann , Christiaens Sigrid , Regenmortel Niels , Gyselaers Wilfried 
Published: March 2022

Abstract
Background The gold standard to measure intra-abdominal pressure (IAP) is via intra-vesical measurement via the urinary bladder. However, this technique is restricted in ambulatory settings because of the risk of iatrogenic urinary tract infections. Rectal IAP measurements (IAPrect) may overcome these limitations, but requires validation. This pilot study aims to validate the IAPrect technique against standard intra-vesical IAP measurements (IAPves).Methods IAPrect using an air-filled balloon catheter and IAPves using Foley Manometer Low Volume were measured simultaneously in sedated and ventilated patients. Measurements were performed twice in different positions (supine and 45° elevated head of bed) and with an external abdominal velcro belt. ResultsSixteen patients were included. Seven were not eligible for analysis due to unreliable IAPrect values. IAPrect was significantly higher than IAPves for all positions (p<0.001) and the correlation between IAPves and IAPrect was not significant in any position (p≥0.25, bias 7.2mmHg, LOA -3.5 to 18mmHg). Repeatability of IAPrect was not reliable (r=0.539, p=0.315). For both techniques, measurements with the external abdominal velcro belt were significantly higher compared to those without (p<0.030).ConclusionsIAPrect has important shortcomings making IAP estimation using a rectal catheter unfeasible because the numbers cannot be trusted nor validated.
AI-enabled Automatic Multimodal Fusion of Cone-Beam CT and Intraoral Scans for Intelligent 3D Tooth-Bone Reconstruction and Clinical Applications
Authors: Hao Jin , Liu Jiaxiang , Lin Hangzheng , Li Jin , Pan Wei , Feng Yang , Chen Ruizhe , Xiong Huimin , Sun Kaiwei , Liu Wanlu , Yang H.Howard , Ding Wanghui , Yang Jianfei , Hu Haoji , Zhang Yueling , Zhao Zeyu , Wu Huikai , Zheng Youyi , Fang Bing , Liu Zuozhu , Zhao Zhihe 
Published: March 2022

Abstract
Cone-beam computed tomography (CBCT) is one of the most widely used digital models in dental practices. A critical step in virtual dental treatment planning is to accurately delineate all tooth-bone structures from CBCT with high fidelity. Previous studies have established several methods for CBCT segmentation using deep learning. However, the inherent resolution discrepancy and the loss of occlusal information in CBCT largely limited its clinical applicability. Here, we present a Deep Dental Multimodal Analysis (DDMA) framework consisting of a CBCT segmentation model, an intraoral scan (IOS) segmentation model (the most accurate digital dental model), and a fusion model to generate 3D fused tooth-bone structures with high fidelity and accurate occlusal information. Our model was trained with a large-scale dataset with 503 CBCT and 28,559 IOS meshes manually annotated by human experts. For CBCT segmentation, our framework achieves an average Dice coefficient of 93.99%, significantly outperforming the baselines. For IOS segmentation, our model achieves a mIoU of 93.07% and 95.70% on the maxillary and mandible on a test dataset of 200 IOS meshes, 1.77% and 3.52% higher than the state-of-art method. In addition, our DDMA framework takes 20 to 25 minutes to generate the fused model, compared to over 5 hours by human experts. Notably, real-world clinical cases demonstrate that our model can visualize tooth-bone structures and relationships during the entire orthodontic treatment and predict clinical risks like dehiscence and fenestration. These findings demonstrate the potential of deep multimodal learning to improve the quality of digital dental models and guide clinical treatment and diagnostic decisions.
A path-based analysis of infected cell line and COVID-19 patient transcriptome reveals novel potential targets and drugs against SARS-CoV-2
Authors: Agrawal Piyush , Sambaturu Narmada , Olgun Gulden , Hannenhalli Sridhar 
Published: March 2022

Abstract
Most transcriptomic studies of SARS-CoV-2 infection have focused on differentially expressedgenes, which do not necessarily reveal the genes mediating the transcriptomic changes. In contrast,exploiting curated biological network, our PathExt tool identifies central genes from thedifferentially active paths mediating global transcriptomic response. Here we apply PathExt tomultiple cell line infection models of SARS-CoV-2 and other viruses, as well as to COVID-19patient-derived PBMCs. The central genes mediating SARS-CoV-2 response in cell lines wereuniquely enriched for ATP metabolic process, G1/S transition, leukocyte activation and migration.In contrast, PBMC response reveals dysregulated cell-cycle processes. In PBMC, the mostfrequently central genes are associated with COVID-19 severity. Importantly, relative todifferential genes, PathExt-identified genes show greater concordance with several benchmarkanti-COVID-19 target gene sets. We propose six novel anti-SARS-CoV-2 targets ADCY2, ADSL,OCRL, TIAM1, PBK, and BUB1, and potential drugs targeting these genes, such as Bemcentinib,Phthalocyanine, and Conivaptan.
Growth Pattern Fingerprinting for Automatic Analysis of Lung Adenocarcinoma Overall Survival
Authors: Alsubaie Najah , Raza Shan E Ahmed , Snead David , Rajpoot Nasir 
Published: March 2022

Abstract
Lung adenocarcinoma (LUAD) tumour tissue grows into variable morphological architecture called growth patterns (GPs). The GPs are clinically linked to the biological behaviour of the tumour. However, due to the complex heterogeneity of the tumours, there is high inter-and intra-observer variability in the pathologist reporting of GPs. In this paper, we propose a deep learning model for the automatic classification of the LUAD growth patterns in whole slide images (WSIs). The model is trained and tested on 78 cases of LUAD in the digitised WSI of the sample. For each case, all the growth patterns were automatically classified and quantified. Our multivariate analysis shows that lepidic and the micropapillary patterns are independent predictors for five-year survival (p<0.05). The proposed model splits our study cohort into short-and long-term survival with p = 0.009.
Resistome characterization of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolated from wastewater treatment utilities in Oregon
Authors: Easler Maeghan , Cheney Clinton , Johnson Jared D. , Zadeh Marjan Khorshidi , Nguyen Jacquelynn N. , Yiu Sue Yee , Waite-Cusic Joy , Radniecki Tyler S. , Navab-Daneshmand Tala 
Published: March 2022

Abstract
Infections resistant to broad spectrum antibiotics due to the emergence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is of global concern. This study characterizes the resistome (i.e., entire ecology of resistance determinants) of 11 ESBL-producing Escherichia coli isolates collected from eight wastewater treatment utilities across Oregon. Whole genome sequencing was performed to identify the most abundant antibiotic resistance genes including ESBL-associated genes, virulence factors, as well as their sequence types. Moreover, the phenotypes of antibiotic resistance were characterized. ESBL-associated genes (i.e., blaCMY, blaCTX, blaSHV, blaTEM) were found in all but one of the isolates with five isolates carrying two of these genes (four with blaCTX and blaTEM; one with blaCMY and blaTEM). The ampC gene and virulence factors were present in all the E. coli isolates. Across all the isolates, 31 different antibiotic resistance genes were identified. Additionally, all E. coli isolates harbored phenotypic resistance to beta-lactams (penicillins and cephalosporins), while 8 of the 11 isolates carried multidrug resistance phenotypes (resistance to three or more classes of antibiotics). Findings highlight the risks associated with the presence of ESBL-producing E. coli isolates in wastewater systems that have the potential to enter the environment and may pose direct or indirect risks to human health.
Effects of Supplementation with Probiotics in Experimental Models of
Alzheimer's Disease: A Systematic Review of Animal Experiments
Authors: Neta Francisca Idalina , de Souza Francisco Emílio Simplício , Batista André Lima , Pinheiro Francisco Irochima , Cobucci Ricardo Ney , Guzen Fausto Pierdoná 
Published: March 2022

Background:
Conditions along the brain-gut-microbiota (BGM) axis can significantly contribute
to the pathogenesis of Alzheimer's disease (AD). Evidence from animal studies indicates a role
of probiotics in regulating mood, cognition, and stress response via the BGM axis. However, the effect
of probiotics on AD needs to be better clarified in preclinical and clinical studies.

Method:
We prepared this systematic review according to PRISMA. PubMed, Web of Science, Embase,
and Virtual Health Library (VHL) were searched for original articles concerning the effects of
probiotics in experimental AD.

Results:
Results were presented as a narrative synthesis according to the Synthesis Without Metaanalysis
(SWiM) Guideline. Seventeen studies met the inclusion criteria. The results showed significant
effects in the experimental models of AD treated with probiotics alone or in mixture due to expressive
improvements in cognitive tests.

Conclusion:
Furthermore, in most of the included studies, it was possible to observe a reduction in inflammatory
processes, an increase in the concentration of peptide hormones, insulin homeostasis in the
brain, increased antioxidant enzymes, and a decrease in beta-amyloid deposition and tau hyperphosphorylation.
Supplementation of probiotics seems to improve performance in cognitive tests and increase
the concentration of substances capable of delaying the neurodegenerative process of AD in experimental
models.
Preliminary In Vitro Evaluation of Some Porphyrins Using Human
Breast Tumor Cells
Authors: Boscencu Rica , Manda Gina , Vasiliu Georgiana , Socoteanu Radu , Lupuleasa Dumitru , Burloiu Andreea Mihaela , Neagoe Ionela Victoria , Olariu Laura 
Published: March 2022

Background::
Tetrapyrrolic compounds, such as porphyrins and metalloporphyrins, are
highly interesting for pharmaceutical chemistry designs considering their good biocompatibility and
therapeutic potential.

Objective::
The aim of the present work was a preliminary in vitro evaluation of some unsymmetrical
porphyrins and the corresponding symmetrical structures as potential candidates for the photodynamic
therapy of malignant tumors.

Methods::
The biocompatibility of compounds was assessed in terms of their in vitro effect on the
viability and proliferation of breast human carcinoma MCF-7 cells and human normal peripheral
blood mononuclear cells.

Results::
Results indicated that unsymmetrical and symmetrical porphyrins were non-toxic against
tumor MCF-7 cells in the concentration range of 0.2–2μM, making them valuable candidates for
further development as photosensitizers for PDT in tumors. Moreover, unsymmetrical compounds
tended to restore the response of normal and tumor cells affected by the vehicle (dimethyl sulfoxide)
used for the initial solubilization of porphyrins, while the symmetrical compounds were less active in
this respect.

Conclusion::
Unsymmetrical A3B type porphyrins prove enhanced capacity as potential theranostic
agents in interaction with human carcinoma MCF-7 cells, despite reduced differences with the corresponding
symmetrical form.
An Overview of the Methodologies and the Quality of Studies on Pharmacotherapy
for the Treatment of Fibromyalgia
Authors: Walker Cristiani Isabel Banderó , Figueiredo Renata Ferraz , Costa Alana Teles , de Oliveira Santos Silva Rafaella , da Silva Tatiana Damasceno , dos Santos Alcântara Thaciana , de Lyra Junior Divaldo Pereira 
Published: March 2022

Background:
It is often unclear whether systematic reviews and primary studies are de-signed to elucidate the efficacy or effectiveness of interventions. This may compromise the use of the information in clinical or policy decisions.

Objective:
This overview aimed to evaluate the methodological profiles of studies on fibromyalgia pharmacotherapy in terms of the quality and nature of the interventions (efficacy versus effective-ness).

Methods:
The protocol was registered in the International Prospective Register of Systematic Re-views database. Seven databases were searched for relevant publications. Systematic reviews inves-tigating the effectiveness or efficacy of fibromyalgia pharmacotherapy were included. Methodolog-ical quality was investigated using A MeaSurement Tool to Assess Systematic Reviews (AM-STAR), and efficacy andeffectiveness were evaluated using Rating of Included Trials on the Effica-cy-effectiveness Spectrum (RITES).

Results:
In this overview, 4,107 studies were initially identified. 8 systematic reviews and 34 prima-ry studies remained after overlaps were removed. Of the eight systematic reviews, 4.76% (n=3) and 7.93% (n=5) were of moderate and high quality, respectively. An analysis of systematic reviews clearly showed the criteria “participants characteristics” and “trial setting" with the most frequent answers as scales 1 and 2 (strong emphasis on efficacy or rather strong emphasis on efficacy), re-spectively. RITES analysis revealed that the most frequent response was “strong emphasis on effi-cacy” in 68% (92/136) of primary studies.

Conclusion:
This analysis showed, in both systematic reviews and primary studies, a predominantly strong emphasis on efficacy, suggesting the need for methodological quality improvement in future studies, especially those designed to provide evidence related to effectiveness.