bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of the time parents spend with children on children's well-being
Authors: Li Dongxu , GUO XI 
Published: April 2022

Abstract
The time with parents is a crucial factor in the growth of children, and children’s well-being is an important indicator of their mental health. To promote the improvement of children's well-being, relying on data from the 2017 China Time Use Survey（CTUS）, this study explores the relationship between parental time and children's well-being and specific influencing factors. The results show that the more time parents spend with children, the higher their children’s well-being. Regarding the types of companionship, the life and leisure time parents spend with children promotes children's well-being. Regarding parent s’ role division, the life and leisure time mother spend with children,and the life and leisure time and educational interactions time father spend with children positively affect children’s well-being. The influence of the time parents spend with children on children’s well-being is heterogeneous based on children’s academic performance. Therefore, family education and guidance services and mental health services should be strengthened, and it is necessary to improve the time spend with children, pay attention to individual diffrences in children.
Twist Springback and Microstructure Analysis of PEEK Sheets in Ultrasonic-Assisted Thermal Incremental Forming
Authors: Liao Juan , Zhou Shaocong , Xue Xin 
Published: April 2022

Abstract
With a view to reducing the springback and twist of PEEK during the incremental forming process, the ultrasonic vibration is introduced into polymer thermal SPIF. In this study, the effects of temperature and ultrasonic vibration on twist and springback of formed parts are investigated. Several modified tool trajectories are developed, and the influence of tool trajectory on twist and springback are also studied. Finally, the microstructures of PEEK parts are analyzed by XRD. The results show that the increase of temperature is helpful to reduce the twist and springback. The ultrasonic vibration is beneficial to the improvement of geometric accuracy, while the side wall bulge aggravates at 140 ℃ after the ultrasonic vibration is applied. The optimized tool trajectory can effectively eliminate twist and reduce the springback of PEEK parts. The XRD result shows that the ultrasonic vibration is helpful to increase the molecular chain orientation, improve crystallinity and refine crystallite at 60 ℃ and 100 ℃. However, the opposite trend occurs at 140 ℃ due to the disorientation effect of molecular chain.
Construction of proliferation-related lncRNA-mRNA regulatory network and lncRNA-miRNA-mRNA ceRNA network by bioinformatics analysis
Authors: Zhuang Xingxing , Zhang Weifeng , Liu Tao , Wei Liangbing , Guo Yanyan , Gao Jiarong 
Published: April 2022

Abstract
Lately, researches on lncRNA-mRNA regulatory network and lncRNA-miRNA-mRNA competitive endogenous RNA (ceRNA) network have been the hotspots, the present study explored the functional roles and regulatory networks of lncRNAs in chronic glomerulonephritis (CGN). The proliferation ability of glomerular mesangial cells (GMCs) induced by different concentrations of lipopolysaccharide (LPS) were detected with CCK-8 assay and RNA-seq was performed to study differentially expressed lncRNAs in LPS-induced GMCs. According to the sequencing results, 6 lncRNAs were selected for RT-qPCR validation. Furthermore, lncRNA-mRNA regulatory network and lncRNA-miRNA-mRNA ceRNA network were constructed to examine the role and mechanism of proliferation-related lncRNAs. To reveal the biological functions of lncRNAs, GO biological process and KEGG pathway analysis were we performed on all mRNAs involed in lncRNA-mRNA regulatory network and ceRNA network. Finally, 1532 differentially expressed lncRNAs, including 594 upregulated lncRNAs and 938 downregulated lncRNAs were screened out by RNA-seq. The results of RT-qPCR validation were consistent with RNA-seq result. The lncRNA-mRNA regulatory network, including 236 lncRNAs and 556 mRNAs and lncRNA-miRNA-mRNA ceRNA network, including 6 lncRNAs, 18 miRNAs, and 419 mRNAs were successfully constructed. The GO biological process and KEGG pathway analysis demonstrated that those lncRNAs were mainly related to inflammatory response and substance metabolism. This study first identified key proliferation-related lncRNAs in LPS-induced GMCs, and further revealed a global view of lncRNA-mRNA regulatory network and lncRNA-miRNA-mRNA ceRNA network involved in CGN. Our findings offered novel insights into the roles of lncRNAs in the pathogenesis of CGN and provided promising diagnostic biomarkers.
International Comparison of Physicians’ Attitudes Toward Refusal of Treatment by Patients with Severe Anorexia Nervosa: A Case-Based Vignette Study
Authors: TAKIMOTO YOSHIYUKI 
Published: April 2022

Abstract
Background: We investigated the attitudes of physicians in Japan, the United Kingdom (UK), and the United States (US) toward refusal of treatment for anorexia nervosa.Methods: A questionnaire survey was administered to eating disorder physicians (Japan, n=55; UK, n=84; US, n=82) to evaluate their treatment strategies for fictitious cases of refusal of treatment for anorexia nervosa.Results: For acute patients, 53 (96.3%) physicians in Japan, 65 (77.4%) in the UK, and 54 (65.9%) in the US chose compulsory treatment if the patient’s family requested treatment, while 46 (83.6%) physicians in Japan, 53 (63.1%) in the UK, and 47 (57.3%) in the US chose compulsory treatment if the family left the decision to the patient. For severe and enduring patients, 53 (96.3%) physicians in Japan, 62 (73.8%) in the UK, and 57 (69.5%) in the US chose compulsory treatment if the patient’s family requested treatment, while 38 (69.1%) physicians in Japan, 56 (66.7%) in the UK, and 55 (67.1%) in the US chose compulsory treatment if the family left the decision to the patient.Conclusions: Physicians in all three countries tended to choose compulsory treatment irrespective of disease duration or whether the patient’s family requested treatment or not. This may indicate that medical practitioners value the ethical obligation of beneficence, giving priority to the protection of life. Attitudes toward refusal of treatment during a life crisis tend to vary among medical professionals, particularly if the patient’s family does not request treatment.
Using Genetically Encoded Fluorescent Biosensors to Interrogate Ovarian Cancer Metabolism
Authors: Bose Shree , Yao Haipei , Huang Qiang , Whitaker Regina , Kontos Christopher D. , Previs Rebecca A. , Shen Xiling 
Published: April 2022

Abstract
BackgroundEpithelial ovarian cancer (OC) is the most lethal gynecological malignancy and patients present with significant metastatic burden, particularly to the adipose-rich microenvironment of the omentum. Recent evidence has highlighted the importance of metabolic adaptations in enabling this metastasis, leading to significant interest in evolving the arsenal of tools used to study OC metabolism. In this study, we demonstrate the capability of genetically encoded fluorescent biosensors to study OC, with a focus on 3D organoid models that better recapitulate in vivo tumor microenvironments. Materials and MethodsPlasmids encoding the metabolic biosensors HyPer, iNap, Peredox, and Perceval were transfected into 15 ovarian cancer cell lines to assay oxidative stress, NADPH/NADP+, NADH/NAD+, and ATP/ADP, respectively. Fluorescence readings were used to assay dynamic metabolic responses to omental conditioned media (OCM) and 100 μM carboplatin treatment. SKOV3 cells expressing HyPer were imaged as 2D monolayers, 3D organoids, and as in vivo metastases via an intravital omental window. We further established organoids from ascites collected from Stage III/IV OC patients with carboplatin-resistant or carboplatin-sensitive tumors (n=8 total). These patient-derived organoids (PDOs) were engineered to express HyPer, and metabolic readings of oxidative stress were performed during treatment with 100 μM carboplatin. ResultsExposure to OCM or carboplatin induced heterogenous metabolic changes in 15 OC cell lines, as measured using metabolic sensors. Oxidative stress of in vivo omental metastases, measured via intravital imaging of metastasizing SKOV3-HyPer cells, was more closely recapitulated by SKOV3-HyPer organoids than by 2D monolayers. Finally, carboplatin treatment of HyPer-expressing PDOs induced higher oxidative stress in organoids derived from carboplatin-resistant patients than from those derived from carboplatin-sensitive patients. ConclusionsOur study showed that biosensors provide a useful method of studying dynamic metabolic changes in preclinical models of OC, including 3D organoids and intravital imaging. As 3D models of OC continue to evolve, the repertoire of biosensors will likely serve as valuable tools to probe the metabolic changes of clinical importance in OC.
Using Global Navigation Satellite Systems for modeling Athletic Performances in elite football players
Authors: Imbach Frank , Ragheb Waleed , Leveau Valentin , Chailan Romain , Candau Robin , Perrey Stéphane 
Published: April 2022

Abstract
This study aims to predict individual Acceleration-Velocity profiles (A-V) from Global Navigation Satellite System (GNSS) measurements in real-world situations.
Data were collected from professional players in the Superleague division during a 1.5 season period (2019-2021).
A baseline modeling performance was provided by time-series forecasting methods and compared with two multivariate modeling approaches using ridge regularisation and long short term memory neural networks. The multivariate models considered commercial features and new features extracted from GNSS raw data as predictor variables. A control condition in which profiles were predicted from predictors of the same session outlined the predictability of A-V profiles. Multivariate models were fitted either per player or over the group of players. Predictor variables were pooled according to the mean or an exponential weighting function.
As expected, the control condition provided lower error rates than other models on average (p = 0.001). Reference and multivariate models did not show significant differences in error rates (p = 0.124), regardless of the nature of predictors (commercial features or extracted from signal processing methods) or the pooling method used. In addition, models built over a larger population did not provide significantly more accurate predictions.
In conclusion, GNSS features seemed to be of limited relevance for predicting individual A-V profiles. However, new signal processing features open up new perspectives in athletic performance or injury occurrence modeling, mainly if higher sampling rate tracking systems are considered.
Early surgical treatment using regional clinical pathways reduces the length of postoperative hospitalization in hip fracture patients: Retrospective analysis using the Japanese Diagnosis Procedure Combination database
Authors: Nishimura Haruki , Suzuki Hitoshi , Tokutsu Kei , Muramatsu Keiji , Kawasaki Makoto , Yamanaka Yoshiaki , Uchida Soshi , Nakamura Eiichiro , Fushimi Kiyohide , Matsuda Shinya , Sakai Akinori 
Published: April 2022

Abstract
Background: Hip fracture is a common injury in elderly people, especially those with weak bone contracture, such as in osteoporosis. Although early surgical treatment is recommended, the optimal timing for hip fracture surgery has not been determined in Japan. To date, regional clinical pathways have been introduced into the clinical field to treat patients in a more effective and unified manner. In this study, we aimed to determine the rate of early surgery among hip fracture patients and the effectiveness of early surgery and the implementation of regional clinical pathways in reducing the length of postoperative hospital stay in hip fracture patients using Japanese Diagnosis Procedure Combination (DPC) database.Methods: Data of patients diagnosed with femoral neck and femoral peritrochanteric fractures retrieved from the Japanese DPC database between April 2016 and March 2018 were used. Patients were divided into early surgery group (43,928 [34%] patients, surgery within 2 days of admission) and delayed surgery group (84,237 [66%] patients, surgery after 2 days of admission).Results: The difference in length of stay (LOS) between the early and delayed surgery groups was 2 days (early vs. delayed: 21.00 days vs. 23.00 days). The early surgery group had more cases of intertrochanteric fractures (57.4% vs. 43.4%) and internal fixation (74.3% vs. 55.4%) than the delayed surgery group. In contrast, the delayed surgery group had more cases of femoral neck fractures (42.6% vs. 56.6%) and bipolar hip arthroplasty (24.5% vs. 41.6%) or total hip arthroplasty (1.2% vs. 3.0%). The early surgery group showed a lower incidence of complications, except anemia (12.0% vs. 8.8%), than the delayed surgery group. Multilevel regression analysis showed that early surgery and implementation of regional clinical pathways reduced LOS by 2.58 and 8.06 days, respectively (p < 0.001). Conclusions: Early surgery was performed in a third of all hip fracture patients. Early surgery and implementation of regional clinical pathways for hip fracture patients are effective in reducing postoperative hospital LOS, with regional clinical pathways having a greater impact. These findings will help acute care providers when treating patients with hip fractures.
Lipid oxidation in cured meat model systems containing either antioxidant or prooxidant: A comparative study on determination of malondialdehyde concentration by using conventional, test-kit and chromatographic assays
Authors: Ozturk-Kerimoglu Burcu , Nakilcioglu Emine , Serdaroglu Meltem 
Published: April 2022

Abstract
The impacts of curing plus antioxidant or prooxidant inclusion in model meat systems on malondialdehyde (MDA) levels detected by three different assays as spectrophotometry (M-1), test kit (M-2), and high-performance liquid chromatography (M-3) were explored. Four different treatments that included non-cured, cured, cured plus zeaxanthin-added, and cured plus ferric iron-added meat systems were produced. MDA levels during storage recorded by M-1 were higher than both M-2 (up to 29-fold) and M-3 (up to 53-fold). The effects of curing were visibly detected by all assays, whereas the impacts of antioxidant or prooxidant addition were much obvious when determined by M-2 and M-3. Despite the variations within numerical values, both M-1 and M-2 presented strong correlations with M-3. Overall, it was concluded that spectrophotometric assays could be reliable on condition that acceptable coefficients are utilized to avoid overestimation. Besides, the simultaneous improvement and simplification of chromatographic techniques as well as rapid test kits would pave the way for their widespread use.
Tip-enhanced Raman spectroscopy with amplitude-controlled tapping-mode AFM
Authors: Umakoshi Takayuki , Kawashima Koji , Moriyama Toki , Kato Ryo , Verma Prabhat 
Published: April 2022

Abstract
Tip-enhanced Raman spectroscopy (TERS) is a powerful tool for analyzing chemical compositions at the nanoscale owing to near-field light localized at a metallic tip. In TERS, atomic force microscopy (AFM) is commonly used for tip position control. AFM is often controlled under the contact mode for TERS, whereas the tapping mode, which is another major operation mode, has rarely been employed despite several advantages, such as low sample damage. One of the reasons is the low TERS signal intensity because the tip is mostly away from the sample during the tapping motion. In this study, we quantitatively investigated the effect of the tapping amplitude on the TERS signal. We numerically evaluated the dependence of the TERS signal on tapping amplitude. We found that the tapping amplitude had a significant effect on the TERS signal, and an acceptable level of TERS signal was obtained by reducing the amplitude to a few nanometers. We further demonstrated amplitude-controlled tapping-mode TERS measurement. We observed a strong dependence of the TERS intensity on the tapping amplitude, which is in agreement with our numerical calculations. This practical but essential study encourages the use of the tapping mode for further advancing TERS and related optical techniques.
Exploring the clinicopathological significance of YKL-39 in gastric adenocarcinoma based on tumor angiogenesis
Authors: Xue Ling , Chu Wei , Wan Fangsheng , Wu Pingfan , Zhao Xiaowen , Ma Linna , She Yali , Li Changtian , Li Yaling 
Published: April 2022

Abstract
Background
Gastric cancer is a malignant tumor with a high incidence and mortality rate. Angiogenesis is necessary for tumor infiltration and metastasis, and also affects patient prognosis. YKL-39 has monocyte chemotactic activity and pro-angiogenic activity in some of the tumors. In this study, we will investigate the relationship between YKL-39 and tumor-associated macrophages and microangiogenesis in gastric cancer and explore the value of YKL-39 as a prognostic biomarker for gastric cancer.
Methods
A total of 119 patients with gastric cancer who had undergone gastrectomy at the 940th Hospital of the Joint Security Force between 2013 and 2019 were included in this study, and the protein expression of YKL-39, CD68, and CD34 was detected by immunohistochemistry, and intracellular expression of YKL-39 and CD68 was detected by immunofluorescence.
Results
Our results showed that YKL-39 was expressed in both nucleus and cytoplasm of gastric cancer tissues and tumor mesenchyme, and the expression of YKL-39 was positively correlated with CD68 and CD34, as well as the high expression of YKL-39 was associated with poor prognosis of gastric cancer patients.
Conclusion
In gastric cancer, YKL-39 expression positively correlated with the degree of tumor-associated macrophage infiltration and angiogenesis, and it could be a potential prognostic marker and therapeutic target for gastric cancer.
Nurses' knowledge, attitudes, and practices about nutritional management of diabetes mellitus
Authors: Farzaei Mahsa , Shahbazi Shahla , Gilani Neda , Ostadrahimi Alireza , Gholizadeh Leila 
Published: April 2022

Abstract
Background: The prevalence of diabetes is increasing rapidly worldwide. Nurses work collaboratively with health care team to improve diabetes management. Yet, little is known about nurses’ role in the nutritional management of diabetes. This study aimed to evaluate nurses’ knowledge, attitude, and practice (KAP) about nutritional management of diabetes. Methods: This cross-sectional study was conducted on 160 nurses recruited between July 4 and July 18, 2021 from two referral tertiary teaching hospitals in Iran. A validated paper-based self-reported questionnaire was used to assess Nurses’ KAP. Nurses’ KAP regarding nutritional management of diabetes were assessed and the associated factors identified using multiple linear regression analysis.Results: The mean knowledge score was 12.16 ± 2.83, with 61.2% of participants showing a moderate knowledge level on nutritional management of diabetes. The mean attitude score was 60.68 ± 6.11, with 86.92% of participants demonstrating positive attitudes. The mean practice score of study participants was 44.74 ± 7.81, with 51.9% having a moderate level of practice. Higher knowledge scores were observed among male nurses (B = -7.55, p = 0.009) and those with blended learning as a preferred learning method (B = 7.28, p = 0.029). Having an opportunity to provide education to patients with diabetes during shifts affected nurses’ attitudes positively (B = -7.59, p = 0.017). Practice scores were higher among nurses who perceived themselves competent in the nutritional management of diabetes (B = -18.05, p = 0.008).Conclusion: Nurses’ knowledge and practice in the nutritional management of diabetes should be increased to help improve the quality of the dietary care and patient education they provide these patients. Further studies are needed to confirm the results of this study both in Iran and internationally.
GAP method: a gap-based technique for outlier detection and its application to wildlife GPS data
Authors: Hirakawa Hirofumi , Muramatsu Daisuke , Gordo Marcelo , Takii Akiko , Izumiyama Shigeyuki 
Published: April 2022

Abstract
Outliers in datasets are a concern for analysts as noise or signals. To detect them, we must quantify how much each data point lies away from others and then set a threshold to separate outliers from the others. However, there is scant research on the latter. We developed a method to address this issue. The method uses value gaps between adjacent data pairs in a univariate dataset, where data are in ascending order of values. Its core process is to find the largest gap in the upper range of the dataset and remove the data above the gap as outliers, and its supplementary process is to repeat the core process with the same dataset after removal. Analysts must decide when to stop this repetition; however, this technique lessens subjectivity by leaving only a few logical options. We can apply this method to any dataset, such as a time series or multivariate dataset, by quantifying data to expose anomalies as outliers in a univariate dataset. We demonstrate how to implement the technique using wildlife GPS data. We expect that this method has a much broader application because, to our knowledge, there are no other techniques to play the role.
Comparison between relative and absolute quantitative real-time PCR applied to single-cell analyses: Transcriptional levels in a key neuron for long-term memory in the pond snail
Authors: Hatakeyama Dai , Chikamoto Nozomi , Fujimoto Kanta , Kitahashi Takashi , Ito Etsuro 
Published: April 2022

Abstract
Quantitative real-time PCR (qPCR) is a powerful method for measuring nucleic acid levels and quantifying mRNA levels, even in single cells. In the present study, we compared the results of single-cell qPCR obtained by different quantification methods (relative and absolute) and different reverse transcription methods. In the experiments, we focused on the cerebral giant cell (CGC), a key neuron required for the acquisition of conditioned taste aversion in the pond snail Lymnaea stagnalis, and examined changes in the mRNA levels of 3 memory-related genes, cAMP-response element binding proteins (LymCREB1 and LymCREB2) and CREB-binding protein (LymCBP), during memory formation. The results obtained by relative quantification showed similar patterns for the 3 genes. For absolute quantification, reverse transcription was performed using 2 different methods: a mixture of oligo d(T) primers and random primers (RT method 1); and gene-specific primers (RT method 2). These methods yielded different results and did not show consistent changes related to conditioning. The mRNA levels in the samples prepared by RT method 2 were up to 3.3 times higher than those in samples prepared by RT method 1. These results suggest that for qPCR of single neurons, the efficacy and validity do not differ between relative and absolute quantification methods, but the reverse transcription step critically influences the results of mRNA quantification.