bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated analysis of intestinal microbiota and metabolomic reveals that decapod iridescent virus 1 (DIV1) infection induces secondary bacterial infection and metabolic reprogramming in Marsupenaeus japonicus
Authors: He Zihao , Zhong Yunqi , Liao Minze , Dai Linxin , Wang Yue , Zhang Shuang , Sun Chengbo 
Published: June 2022

AbstractIn recent years, with global warming and increasing marine pollution, some novel marine viruses have become widespread in the aquaculture industry, causing huge losses to the aquaculture industry. Decapod iridescent virus 1 (DIV1) is one of the newly discovered marine viruses that has been reported to be detected in a variety of farmed crustacean and wild populations. Previous studies have found that DIV1 can induce the Warburg effect. To further explore the effect of DIV1-induced metabolic reprogramming on Marsupenaeus japonicus intestinal metabolome and microbiota and the consequence on immune response, histological analysis, enzyme activity analysis and the integrated analysis of intestinal microbiome and metabolomics were performed in this study. The results showed that obvious injury in the intestinal mucosa was observed after DIV1 infection. The oxidative and antioxidant capacity of the shrimp intestine was unbalanced, the activity of lysozyme was decreased, and the activities of digestive enzymes were disordered, causing secondary bacterial infection. In addition, the increased abundance of harmful bacteria, such as Photobacterium and Vibrio, synergized with DIV1 to promote the Warburg effect and induce metabolic reprogramming, thereby providing material and energy for DIV1 replication. This study is the first to report the changes of intestinal microbiota and metabolites of M. japonicus under DIV1 infection, demonstrating that DIV1 can induce secondary bacterial infection and metabolic reprogramming, and several highly related bacteria and metabolites were screened as biomarkers. These biomarkers can be leveraged for diagnosis of pathogenic infections or incorporated as exogenous metabolites to enhance immune response.
Neutrophils reprograms the bone marrow to impair T-cell immunity during tuberculosis
Authors: Saqib Mohd. , McDonough Elizabeth , Das Shreya , Cheng Zhongshan , Sankar Poornima , Li Fuxiang , Yang Qianting , Xu Yuzhong , Wang Weifei , Chen Xinchun , Ojha Anil K , Ginty Fiona , Cai Yi , Mishra Bibhuti B 
Published: June 2022

ABSTRACTMycobacterium tuberculosis (Mtb) infection induces persistent influx of neutrophils that associates with poor bacterial control and clinical outcome from tuberculosis (TB). Although implicated in TB pathology, the mechanism by which these cells contribute to pathogenesis is poorly understood. Using Cell-DIVE multiplexed immunofluorescence imaging and spatial analysis of inflammatory TB lesions, we demonstrated that persistent neutrophil infiltration affects the spatiotemporal organization of T-lymphocytes and impairs their function. Instead of directly suppressing T-cells, neutrophils produce granulocyte colony stimulating factor (CSF3/G-CSF) that collaborates with type I interferon (IFN-I) to promote a granulocyte-skewed hematopoiesis impacting T-lymphocyte production. Importantly, neutrophil-intrinsic IFN-I receptor 1 (IFNAR1) is both necessary and sufficient to promote pathologic granulopoiesis. Finally, inhibition of IFNAR1-signaling alone mitigates immunopathogenesis by restoring hematopoietic equilibrium. Collectively, our work uncovers a potential immunevasion strategy by which virulent Mtb strains induce IFN-I to generate pathogen-permissive neutrophils that produce G-CSF and sustain pathogenic hematopoiesis to impair T-cell immunity during TB.
The association between mental status, personality traits, and discrepancy in social isolation and perceived loneliness among community dwellers
Authors: Watanabe-Miura Kumi , Sekiguchi Takuya , Otake-Matsuura Mihoko 
Published: June 2022

AbstractObjectivesTo examine the factors associated with social asymmetry, which refers to the discrepancy between actual social isolation and perceived loneliness, focusing on an individual’s mental status and personality traits.MethodsThis study introduced a cross-sectional study design that was utilizing the data from the University of Michigan Health and Retirement Study (HRS) from waves during 2014 and 2016. The participants were community dwellers aged 50 years and older. The outcome measurement, social asymmetry, was defined as the discrepancy between social isolation according to six criteria and loneliness as assessed by the three-item version of the Revised UCLA Loneliness Scale. Multinomial logistic regression models were conducted to examine the factors associated with social asymmetry.ResultsFewer depressive symptoms and higher extraversion were consistently associated with social asymmetry, compared with both isolation and loneliness. Participants with higher neuroticism were likely to be lonely even in the absence of isolation, whereas those with lower neuroticism were likely to not be lonely even with social isolation. In addition, participants with fewer depressive symptoms, lower neuroticism, and higher extraversion were more likely to be not lonely even with social isolation, compared with lonely even in the absence of isolation.ConclusionsMental status and personality traits may closely relate to social asymmetry. This study suggests that incorporating social, mental, and psychological factors may be essential for interventions in social isolation and loneliness.HighlightsSocial asymmetry is a phenomenon reflecting the discrepancy between actual social isolation and perceived loneliness. This gap between situation and emotion may be associated with health risks. However, little information is available on social asymmetry, and its related factors remain poorly understood. Thus, we examined the factors associated with social asymmetry using data from the University of Michigan Health and Retirement Study (HRS). We hypothesized that both mental status and personality traits play a role in social asymmetry due to their effect on individuals’ perceptions.As a result, depressive symptoms, neuroticism, and extraversion, as well as demographic and socioeconomic status were consistently associated with both social asymmetry outcomes: 1) only social isolation (without loneliness) and 2) only loneliness (without social isolation). This result implies that mental and psychological factors were associated with social asymmetry in a complex manner and that incorporating social, mental, and psychotherapeutic aspects in social interventions may be essential for future intervention strategies for social isolation and loneliness.
An investigation of key precursors of megaproject crises
Authors: Wang Aimin 
Published: June 2022

PurposeDetecting precursory signals is critical to increasing the resilience of megaprojects to crises. This study aims to investigate key crisis precursors to better apprehend traces of megaproject crises and proactively respond to approaching crises, thereby increasing project resilience.Design/methodology/approachDrawing on the theory of weak signals, 25 potential precursors of megaproject crises are extracted. Using exploratory structural equation modeling (ESEM), four models were tested to identify key crisis precursors based on a questionnaire survey of 168 megaproject participants in China. Analysis of variance (ANOVA) was employed to examine the differences in participants’ perceptions of key crisis precursors.FindingsFour key crisis precursors were identified: Imbalance of Benefits and Costs, Irresponsible and Unqualified Key Participants, Regulatory Mechanisms Lacking Transparency and Flexibility, and Information Uncertainty and Asymmetry. Compared with senior executives, team members think that key crisis precursors related to regulatory mechanisms and key participants are more likely to trigger crises, which affects responsive actions toward detected crisis precursors. The findings help identify problems at an early stage and enable timely intervention to mitigate crises.Research limitations/implicationsDetection of key crisis precursors allows for proactive and targeted actions to mitigate crises. Megaproject development is affected by institutional mechanisms. The influence of institutions on the effectiveness of crisis management needs further investigation.Originality/valueLimited attention has been paid to recognizing precursors of megaproject crises. This study identifies four key crisis precursors by uncovering the interrelationships among potential precursors, which enables rapid adaptation to future crises.
Analysing the sustainability competencies of preservice teachers in Spain
Authors: Baena-Morales Salvador , García-Taibo Olalla , Merma-Molina Gladys , Ferriz-Valero Alberto 
Published: June 2022

PurposePhysical education has been described as an essential material to contribute to sustainable development. Therefore, it is necessary to examine the proficiency of higher education students in the three dimensions of sustainable development. This paper aims to analyse both the competences associated with the sustainability of preservice physical education teachers and the curriculum's influence on them.Design/methodology/approachIn total, 341 students (112 women) completed the validated Physical Education for Sustainable Development – Future Teacher PESD-FT questionnaire. This instrument assesses sustainable competences in its three dimensions (social, economic and environmental). In order to determine the influence of the curriculum on sustainability competences, the academic year studied was used as a variable. Variables such as gender, age and competitive experience in institutionalised sport were also considered in the statistical analysis.FindingsThe results showed that the total set of students scored above average in their self-perception of competences associated with sustainable development in all three dimensions. The Mann–Whitney U test and Kruskal–Wallis statistics showed no differences attributed to gender and grade, respectively.Originality/valueThis could help to rule out the influence of the curriculum as the main factor in the acquisition of these sustainable competences. However, greater competitive experience in institutionalised sports was associated with greater proficiency in social competence within sustainable development.
The impact of digital trust on firm value and governance: an empirical investigation of US firms
Authors: Kluiters Leon , Srivastava Mohit , Tyll Ladislav 
Published: June 2022

PurposeThis study aims to investigate the effects of firm- and governance-specific characteristics on digital trust (DT) and firm value. Firm-specific factors include return on assets (ROA), market-to-book ratio (M/B ratio), size and leverage, whilst governance-related factors comprise board size, percentage of female board members, board independence and institutional ownership. All listed US firms over the period of 2011–2016 were analysed in this study.Design/methodology/approachThis study provides a novel method to empirically measure DT by combining multiple variables to create a combined DT score. The variables include security and privacy scores, security rankings and data breaches, amongst others. Subsequently, a linear regression was performed to evaluate the effect of firm- and governance-specific characteristics on DT, as well as the effect of DT on firm value.FindingsBy using signalling theory, this study finds significant evidence that a firm’s profitability (ROA) decreases whilst its size increases DT. This could be due to the fact that firms with lower DT monetise data more actively, decrease DT and increase short-term profitability. Significant evidence also shows that increasing DT leads to an increase in firm value.Originality/valueAlthough numerous studies have been conducted on developing customers’ trust by incorporating corporate social responsibility to improve firm value, the literature remains still on its digital analogue. Therefore, this study extends the knowledge of corporate digital responsibility (CDR) by providing a novel method for calculating DT across industries as an antecedent of CDR. Specifically, it sheds light on how firms can enhance DT by utilising firm- and governance-level factors. This enhanced DT can subsequently increase firm value. The study provides important managerial implications by providing empirical evidence that cybersecurity investments increase firm value. This value increase is related to the rise in shareholder value amongst investors and the increase in the organisation’s consumer perceptions as the latter’s interests are better managed.
Epidemiological and hematological investigation of dengue virus infection
Authors: Anwar Faheem , Ullah Sami , Aziz Aziz ur Rehman , Rehman Abid Ur , Khan Jawad , Tayyab Muhammad , Haq Ihteshamul , Mahmood Muhammad Tariq 
Published: June 2022

AbstractDengue is a mosquito‐borne viral illness that infects humans. For the past few decades, it has been declared a global public health problem. The current study, conducted at the district headquarter hospital (DHQ) Bannu between June to September 2018, was based on the seroprevalence of antibodies against dengue virus serotypes and their hematological parameters among the patients. A total of 1738 individuals suspected of having dengue were diagnosed through NS1, IgG, and IgM antibodies and RT‐PCR techniques. Of all the samples, 716 (41.19%) were found to be positive for dengue. A higher infection rate was found in males (65.92%) compared with females (34.07%). The most affected age group was 16–40 years, whereas the most affected tehsil was Bannu, where the DENV‐3 serotype was prevalent. The rare serotype (DENV‐4) was found in 1% of cases. Symptoms including fever (100%), myalgia (100%), headache (61.31%), vomiting (34.63%), and rashes were common among the dengue patients. However, the mild cases showed fewer clinical signs compared with the severely infected cases. The study also revealed a significant association (P < 0.05) between hematological parameters and dengue infection, showing a significant decrease in TC, eosinophils, neutrophils, and platelets and a significant increase in monocytes and lymphocytes. Based on the current report, it is concluded that patients with the above symptoms and hematological changes may have an increased probability of dengue and should be kept under observation to separate dengue‐positive patients and to enhance the treatment process.
Genetic characterization of the cyclohexane carboxylate degradation pathway in the denitrifying bacterium Aromatoleum sp. CIB
Authors: Sanz David , Díaz Eduardo 
Published: June 2022

AbstractThe alicyclic compound cyclohexane carboxylate (CHC) is anaerobically degraded through a peripheral pathway that converges with the central benzoyl‐CoA degradation pathway of aromatic compounds in Rhodopseudomonas palustris (bad pathway) and some strictly anaerobic bacteria. Here we show that in denitrifying bacteria, e.g. Aromatoleum sp. CIB strain, CHC is degraded through a bad‐ali pathway similar to that reported in R. palustris but that does not share common intermediates with the benzoyl‐CoA degradation pathway (bzd pathway) of this bacterium. The bad‐ali genes are also involved in the aerobic degradation of CHC in strain CIB, and orthologous bad‐ali clusters have been identified in the genomes of a wide variety of bacteria. Expression of bad‐ali genes in strain CIB is under control of the BadR transcriptional repressor, which was shown to recognize CHC‐CoA, the first intermediate of the pathway, as effector, and whose operator region (CAAN4TTG) was conserved in bad‐ali clusters from Gram‐negative bacteria. The bad‐ali and bzd pathways generate pimelyl‐CoA and 3‐hydroxypimelyl‐CoA, respectively, that are metabolized through a common aab pathway whose genetic determinants form a supraoperonic clustering with the bad‐ali genes. A synthetic bad‐ali‐aab catabolic module was engineered and it was shown to confer CHC degradation abilities to different bacterial hosts.
Colorado Basin Incentive‐Based Urban Water Policies: Review and Evaluation
Authors: Colby Bonnie G. , Hansen Hannah 
Published: June 2022

ABSTRACTMajor cities located in the Colorado River Basin (CRB) rely on incentive‐based policies to address water use and supply reliability challenges, through programs provided by cities themselves, by state and federal agencies, and by NGOs. This review examines water use trends across cities, the phenomena of declining per capita use, and finds that most large cities have adopted municipal rate structures designed to incentivize lower use. A number of urban areas provide incentives to use gray water and effluent for outdoor use and to harvest rainwater. Incentive‐based programs to protect watershed health have become a water supply strategy implemented through programs and partnerships across the CRB. The paper concludes by reviewing the ways that incentive‐based urban water policies are being evaluated, and by providing guidelines for designing and evaluating programs to reduce urban use and protect watersheds that provide urban supplies.
Erlotinib with or without bevacizumab as a first‐line therapy for patients with advanced nonsquamous epidermal growth factor receptor‐positive non‐small cell lung cancer: Exploratory subgroup analyses from the phase II JO25567 study
Authors: Hosomi Yukio , Seto Takashi , Nishio Makoto , Goto Koichi , Yamamoto Noboru , Okamoto Isamu , Tajima Kosei , Kajihara Yusuke , Yamamoto Nobuyuki 
Published: June 2022

AbstractBackgroundIn the phase II JO25567 study (JapicCTI‐111390), erlotinib plus bevacizumab demonstrated a significant clinical benefit in Japanese patients with epidermal growth factor receptor mutation‐positive (EGFR+) non‐small cell lung cancer (NSCLC). Here, we present an exploratory analysis investigating the impact of baseline pleural/pericardial effusion (PPE) on patient outcomes.MethodsPatients with stage IIIB/IV or postoperative recurrent EGFR+ NSCLC were randomized 1:1 to receive erlotinib (150 mg/day) plus bevacizumab (15 mg/kg every 3 weeks) or erlotinib monotherapy. Progression‐free survival (PFS), overall survival (OS), objective response rate (ORR), and safety were evaluated according to the presence or absence of baseline PPE.ResultsThe population comprised 152 patients, 66 with baseline PPE and 86 without. Median PFS was longer with erlotinib plus bevacizumab than with erlotinib alone, with (hazard ratio [HR] 0.45; 95% confidence interval [CI]: 0.25–0.82) or without (HR 0.62; 95% CI: 0.37–1.04) baseline PPE. Median OS was also prolonged with erlotinib plus bevacizumab relative to erlotinib regardless of the presence (HR 0.82; 95% CI: 0.46–1.47) or absence (HR 0.84; 95% CI: 0.46–1.55) of baseline PPE. ORR was higher with erlotinib plus bevacizumab (70.0%) than with erlotinib (55.6%) in patients with baseline PPE, but similar (68.9% vs. 70.7%) in patients without. Most common grade ≥3 adverse events were hypertension and rash in the erlotinib plus bevacizumab arm, and rash in the erlotinib arm, regardless of baseline PPE status.ConclusionsErlotinib plus bevacizumab may be a beneficial treatment strategy in patients with EGFR+ NSCLC, especially for those with baseline PPE.
Shaping ability of different NiTi rotary systems during the preparation of printed mandibular molars
Authors: Falakaloğlu Seda , Silva Emmanuel João Nogueira Leal , Yeniçeri Özata Merve , Gündoğar Mustafa 
Published: June 2022

AbstractThis study aimed to evaluate the shaping ability of XP‐endo Shaper, TruNatomy and EdgeFile X3 during the preparation of resin‐printed mandibular molar mesial root canals. Thirty‐three resin‐based mandibular mesial roots with two canals, obtained from extracted tooth cone‐beam computed tomography (CBCT) image and printed on a three‐dimensional (3D) printer, were divided into three experimental groups according to the different nickel–titanium (NiTi) systems used for root canal preparation. The specimens were scanned using CBCT imaging before and after root canal preparation. Then images were registered using a dedicated software and changes in the canal area, volume, untouched canal surface and the maximum and minimum dentine wall wear were calculated. The XP‐endo Shaper instruments showed improved shaping ability with lower untouched root canal surface and better preservation of root canal anatomy during the preparation of resin‐printed mandibular mesial root canals compared with TruNatomy and EdgeFile X3 systems.
Butterflies' biodiversity of Souk Ahras Forest (Mechroha and Ouled Driss North east of Algeria)
Authors: Saouli Hamza , Boucif Abdelhak , Djabourabi Ryma , Soualah Alila Hana 
Published: June 2022

AbstractThis study investigated for the first time species richness, abundance of butterflies and disturbed habitats in Souk Ahras Forest. The assessment of butterflies' diversity and relative abundance was conducted from July 2019 to July 2021. We recorded 60,696 individuals of 49 butterfly species belonging to six families (Papilionidae, Pieridae, Lycaenidae, Nymphalidae, Hesperiidae and Sphingidae). Nymphalidae was dominant by 19 species, followed by Lycaenidae 13 species, Pieridae 10 species, Hesperiidae 4 species, Papilionidae 2 species and diurnal moths ‘Sphingidae’ with only one species. The highest number of species was recorded in oaks groves (medium high altitude) with 35 species; in terms of abundance, grassland (low altitude) dominates in number (n = 20,094). The lowest species diversity (S = 7) and number of individuals (n = 1227) were recorded in the Dense tree stratum scrub station which records significant water pollution. We notice that the abundance of butterflies was important during the spring and summer periods but less important during the winter. Landscape composition and habitat influence butterfly diversity and their dynamics. Many species used more than one distinct habitat type, or just one special habitat; we suggest that vegetation cover is an important mechanism for butterfly diversity persistence in forest ecosystems mosaics.
Short recce transects or camera trap surveys—Short recce surveys highlighted as a useful supplement for rapid biodiversity assessments in the Republic of the Congo
Authors: Gaugris Jerome , Orban Ben , Niemand Lukas , Walsh Gina , Burger Marius , Morley Robert , Melville Haemish , Drescher Karsten , Kabafouako Gérard , Vasicek Gaugris Caroline 
Published: June 2022

AbstractA rapid biodiversity assessment was conducted in the Republic of the Congo including a systematic biodiversity study (including a targeted study of mammals), implemented over two seasons. One hundred thirteen recce transects (250 m by 2 m) were undertaken over 34 days of fieldwork. A total of 19 species were detected (3 to genus level only). The encounter rate was 26.65 mammalian signs per km; no differences in numbers of signs between season appeared; however, the dry‐season species accumulation pattern was higher than during wet season. Correlation analysis showed increasing density of mammals as distance away from villages, lakes and coast increased. The correlation result is deemed to highlight not only a substantial hunting pressure and avoidance of humans, but also preference for forested habitats noted to appear from 20 km distance from the coast. Using high numbers of relatively short recce transect proved a useful supplementary method for assessing mammalian diversity during rapid biodiversity assessments. Results suggest that 70 recce transects (with a minimum of eight per habitat type) would have been sufficient to yield this information.