bluuebunny/crossref_metadata_2025_split huggingface dataset:
Introduction of versatile and recyclable network poly (ionic liquid)s as an efficient solvent with desired properties for application in C-C cross-coupling reactions
Authors: Patra Indrajit , Musa Duaa Abdul Rida , Solanki Reena , Mustafa Yasser Fakri , Yakhshieva Zukhra Ziyatovna , Hadi Jihad M. , Kazemnejadi Milad 
Published: August 2022

AbstractSynthesis of high viscosity, ecofriendly, and low melting point poly(ionic liquid)s (LMP-PIL) with network structure has been introduced by the reaction of TAIm[I] ionic liquid monomer with disodium salts of various open-chain carboxylic acids as well as sulfonic acidsviaa polycondensation on counter ions (PC-CI). Changes in methylene group numbers of carboxylate, as well as sulfonate, provide a broad spectrum of properties in terms of viscosity, density, and solubility for the resulting PILs. The physical properties of the PILs including, viscosity, melting point, boiling point, density,M¯n, and DPI, were determined in this study. The ecofriendly PILs served as an efficient solvent and ligand for the Cu-catalyzed Heck and Sonogashira C-C coupling reactions. High to excellent yields were obtained for various coupling products under mild conditions at MP of the PILs. The prepared PILs could be recycled several times from the homogenous reaction mixture with preservation of the intrinsic properties. Also, a deep study was conducted on the mechanism of the coupling reactions.
A study of healthcare provided to diabetic patients during the Syrian crisis in Damascus (Health awareness, Drug therapy, Therapeutic follow-up)
Authors: Alchatti Omar , Shamaa Majd , Al-Fawaris Ma'moun , Hammoud Taghrid 
Published: August 2022

AbstractBackground: This study aims to assess the level of health care for patients with diabetes in Damascus during the Syrian crisis by studying the level of health awareness and its relationship to the levels of Hemoglobin A1c, and studying the availability of drug therapy and the quality of treatment follow-up provided for patients. Methods and materials: A descriptive cross-sectional study, that used a paper questionnaire filled out by means of a mini interview, the sample was selected from patients with diabetes who were in the outpatient department of Damascus Hospital and the endocrinology clinic at the Martyr Bassam Abboud Medical Center. Results: The study included 385 patients (56.6% males and 43.4% females) and 48.05% of the participants were in the age group of 40–60 years. The level of health awareness among patients was good, especially with regard to information about the role of diet and exercise in diabetes, knowledge of interpreting the results of blood sugar tests and methods to prevent hyperglycemia and hypoglycemia attacks. No relationship was found between the high level of health awareness and decreased values ​​of Hemoglobin A1C in the participants. 74.3% of the participants expressed that they do not face difficulties in order to acquire treatment, as they used to get it for free from health centers. The majority of patients checked their blood sugar level continuously and checked the levels of Hemoglobin A1c, kidney function tests, lipids and cholesterol levels periodically. Conclusion: The level of health awareness for diabetic patients in Damascus is generally average, and there are no difficulties in securing treatment for the majority of patients, and the therapeutic follow-up of the disease was at a high level, as laboratory tests required for diabetes are carried out periodically and regularly.
N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysis
Authors: Yuan Bao , Zhou Jin 
Published: August 2022

AbstractIt has been reported that N6-methyladenosine (m6A) modification is mainly involved in clear-cell renal cell carcinoma (ccRCC) tumorigenesis. However, the regulatory mechanisms of m6A reader IGF2BP1 involved in ccRCC tumor energy metabolism are currently unknown. Results showed that the m6A reader IGF2BP1 exhibited significantly higher expression in ccRCC cells. Functionally, results by gainloss functional assay indicated that IGF2BP1 promoted the glucolytic characteristics, including glucose uptake, lactate production and extracellular acidification rate (ECAR). Mechanistically, IGF2BP1 recognizes the m6A modified sites on LDHA mRNA and enhances its mRNA stability, thereby accelerating tumor energy metabolism. Thus, our work reveals a novel facet of the m6A that promotes mRNA stability and highlights the functional importance of IGF2BP1 as m6A readers in post-transcriptional gene regulation.
N6-methyladenine demethylase ALKBH5 alleviates cardiomyocytes apoptosis via modulating m6A/SIRT1 manner
Authors: Liu Liangliang , Liu Zhen 
Published: August 2022

Abstract
Recent studies have shown that the potential regulatory role of N6-methyladenine (m6A) modification may affect the occurrence and development of various cardiovascular diseases. However, the regulatory mechanism of m6A modification on myocardial ischemia reperfusion injury (MIRI) is rarely reported. A mice model of myocardial I/R was established by ligation and perfusion of the left anterior descending coronary artery, and a cellular model of H/R was conducted in cardiomyocytes (CMs). We found that the protein expression of ALKBH5 in myocardial tissues and cells were decreased, accompanied by increased m6A modification levels. Overexpression of ALKBH5 significantly inhibited H/R induced oxidative stress and apoptosis in CMs. Mechanistically, deacetylases Sirtuin-1(SIRT1), a master regulator of oxidative stress, apoptosis and endothelial function genes, at enriched m6A motif in the 3’-UTR, which promotes the stability of SIRT1 mRNA. Furthermore, overexpression or knockdown of SIRT1 confirmed the protective effect of SIRT1 on H/R induced CMs apoptosis. Together, our study reveals a critical mechanism between ALKBH5-medicated m6A and CM apoptosis, supplying an important regulating effect of m6A methylation in ischemic heart disease.
Power-Up Stable CMOS DC Offset Cancellation Circuit for Programmable Gain Lowpass Filters
Authors: Yesil Ercem , Zencir Ertan 
Published: August 2022

Abstract

In this study, a 5th-order active-RC lowpass filter with a power-up stable DC offset cancellation (DCOC) circuit is designed. The filter achieves a 120 MHz cutoff frequency with a 4-bit programmable 0-18 dB gain. The DCOC circuit is utilized for the last stage of the filter and can compensate for a 3σ, 128 mV offset voltage to 1.2 mV in the maximum gain setting. The settling time of the DCOC circuit is approximately 50 μs. DCOC introduces a 46 kHz and 11.3 kHz lower cutoff frequencies for maximum and minimum gain settings respectively. The design is realized in the commercial ST 130 nm PD SOI process, and the main supply voltage is 1.2 V along with 1.8 V utilized in a section of DCOC. The total power consumption of the design is 21 mW with a 590 μs x 280 μs active layout area for the design.
Da Vinci robot-assisted pylorus- and vagus nerve-preserving gastrectomy for early gastric cancer: A single-center study
Authors: Fan Yichuan , Wei Maohua , Zhang Chi , Liang Pin , Hu Xiang 
Published: August 2022

Abstract
Background
This study aimed to assess the efficacy, feasibility, and safety of robot-assisted pylorus- and vagus nerve-preserving gastroplasty (RA[PPG]), using the da Vinci Surgical System, compared with those of laparoscopy-assisted PPG (LAPPG).
Methods
Clinical and follow-up data of patients who underwent PPG were retrospectively analyzed, which included 14 RAPPG and 21 LAPPG cases. The histological data, surgical results, postoperative recovery, and complication rates were compared between the two groups; the surgical experience was also summarized.
Results
The operation time was slightly longer in the RAPPG group (309.3 ± 59.9 vs. 236.2 ± 36.5 min, P < 0.05), but this group had less intraoperative bleeding. The length of the preserved pyloric canal, number of dissected lymph nodes, postoperative recovery, and overall complication rates were not significantly different. The proportion of delayed gastric emptying and acid reflux symptoms in the LAPPG group was slightly greater than that in the RAPPG group.
Conclusion
The da Vinci Surgical System is a safe and feasible way to control the postoperative gastric emptying speed and to effectively reduce reflux.
Preserving the medial arm of the iliofemoral ligament in total hip arthroplasty using the anterolateral approach: A retrospective analysis of patient-reported outcome measure with 2 years follow-up
Authors: Harada Yoshifumi , Yamamoto Yuji , Oishi Kazuki , Inoue Ryo , Akaishi Koichi , Ishibashi Yasuyuki 
Published: August 2022

Abstract
Purpose
Iliofemoral ligament (ILFL) is a capsular ligament located in the anterosuperior part of the hip joint capsule and contributes to hip joint stability. The purpose of this study was to compare the postoperative outcomes of resecting and preserving the medial arm of the ILFL in primary total hip arthroplasty (THA) using the anterolateral approach.
Methods
Patients with unilateral hip joint disease were selected, including 26 in the resection group and 23 in the preservation group. As a patient-reported outcome measure (PROM), the Japanese Orthopaedic Association Hip Disease Evaluation Questionnaire was administered at 3 months, 6 months, 1 year and 2 years postoperatively.
Results
The visual analog scale of satisfaction about the hip was significantly higher in the preservation group than in the resection group at 3 months (93.9 vs 80.8, P = 0.005), 6 months (94.3 vs 80.3, P = 0.001), and 1 year (94.0 vs 82.1, P = 0.001) postoperatively. The pain score of PROM was significantly superior in the preservation group than in the resection group at 3 months (25.2 vs 22.2, P = 0.024), 6 months (25.9 vs 21.8, P < 0.001), and 1 year (25.3 vs 21.8, P = 0.004) postoperatively. The mental score of PROM was significantly superior in the preservation group than in the resection group at 3 months (21.1 vs 17.7, P = 0.032), 6 months (23.3 vs 20.7, P = 0.038), and 1 year (23.3 vs 20.5, P = 0.027) postoperatively.
Conclusion
Our results demonstrated the effectiveness of preserving the medial arm of the ILFL in THA using the anterolateral approach for improvement of PROMs for 1 year postoperatively.
A narrow host-range and lack of persistence in non-target insect species of symbiotic bacteria exploited to deliver insecticidal RNAi in Western Flower Thrips
Authors: Whitten Miranda M.A. , Xue Qi , Taning Clauvis Nji Tizi , James Reuben , Smagghe Guy , Sol Ricardo Del , Dyson Paul 
Published: August 2022

Abstract
Insecticidal RNAi is a targeted pest insect population control measure. The specificity of insecticidal RNAi can theoretically be enhanced by using symbiotic bacteria with a narrow host range to deliver RNAi, an approach termed symbiont-mediated RNAi (SMR), a technology we have previously demonstrated in the globally-invasive pest species Western Flower Thrips (WFT). Here we determine a very limited distribution of the two predominant bacterial symbionts of WFT, BFo1 and BFo2, among genome-sequenced insects. Moreover, we have challenged two non-target insect species with both bacterial species, namely the pollinating European bumblebee, Bombus terrestris, and an insect predator of WFT, the pirate bug Orius laevigatus. Our data indicate that whereas BFo1 could establish itself in both these non-natural hosts, albeit with no significant effects on insect fitness, BFo2 was unable to persist in either species. In terms of biosafety, these data, together with the more specific growth requirements for BFo2, vindicate the choice of this species for precision pest management of WFT using SMR.
Laparoscopic versus robot-assisted radical prostatectomy: Comparison of outcomes of a single surgeon
Authors: Estrela João Rafael Libório , Ziomkowski Alexandre Azevedo , Leão Nilo Jorge , Fernandes Laís Araújo , Gomes Perlla Cerqueira Couto , Calazans Leonardo 
Published: August 2022

Abstract
Objective
To compare the functional and oncological results of laparoscopic radical prostatectomy (LRP) and RALP performed by a single surgeon.
Methods
This retrospective cohort study included patients who underwent LRP and RALP performed by a single surgeon between June 2017 and April 2020 and were followed-up for 12 months. The Da Vinci ® robotic system was used for all surgeries. The potency, continence, surgical margins, postoperative prostate specific antigen (PSA) level, and surgical complications were compared.
Results
The cohort included 156 patients: 103 and 53 patients underwent RALP and LRP, respectively. We found that RALP were superior to LRP with respect to potency in all periods analyzed. At 3 months, 60% of the patients in the RALP group were potent, and this proportion rose to 87.1% at the end of one year, versus 36.6% and 66.7% at the corresponding periods in the LRP group. No statistical differences were observed in the surgical margins, post-operative PSA, or continence.
Conclusion
The comparison of the RALP and LRP performed by the same surgeon revealed the superiority of the former over the latter, with respect to the postoperative sexual potency. There was no difference in the continence rates, surgical margin, or persistence of disease.
Changes in IL-27 and its effect on CD4 + T cells in patients with coronary artery disease
Authors: Cai Yifan , Tang Hongxia , Tang Wenjuan , Xu Wenbin , Wang Yue , Ding Yan , Yu Jian , Pan Chengliang , Li Zhiyang , Peng Yudong , Zhu Ruirui , Yu Kunwu , Zeng Qiutang , Zhong Yucheng 
Published: August 2022

Abstract
Objective：Coronary artery disease (CAD) is an immune-mediated chronic disease, and interleukin-27(IL-27) regulates CD4+ T cell differentiation. However, little is known about its effects on CAD. Therefore, we aimed to investigate the changes of IL-27 and its effect on CD4+ T cells in patients with CAD.Methods: The severity of coronary artery stenosis was assessed by Gensini score, and the concentrations of plasma IL-27, ox-LDL were measured by ELISA. qRT-PCR and Western Blotting (WB) were performed to evaluate the mRNA and protein expression of T-bet, IFN-γ, GATA-3, and RORγt. After monocytes were stimulated with recombinant IL-2 and/or IL-27, CD4+IFN-γ+T cells, CD4+IL-4+T cells, CD4+IL-17+T cells, CD4+LAP+T cells and CD4+CD25+Foxp3+ Tregs were counted by flow cytometry. Results: Plasma IL-27 levels were significantly elevated in patients with Acute Coronary Syndromes (ACS). IL-27 levels were positively correlated with ox-LDL and Gensini scores (P < 0.01) and ox-LDL levels were positively correlated with Gensini scores (P < 0.01). The more severe the stenosis in CAD patients, the more Th1 and Th17 cells, and the less Th2, CD4+CD25+Foxp3+Tregs and CD4+LAP+T cells. IL-27 can increase the expression of T-bet and IFN-γ, and inhibit the expression of RORγt and GATA-3, and finally promote the differentiation of CD4+T cells into Th1 cells, and inhibit the differentiation of Th2, Th17, CD4+CD25+Foxp3+Tregs and CD4+LAP+T cells.Conclusion: IL-27 regulates CAD by increasing the expression of T-bet and IFN-γ and inhibiting the expression of RORγt and GATA-3, thereby increasing the frequency of Th1 cells and decreasing the frequency of Th2, Th17, CD4+CD25+Foxp3+Tregs and CD4+LAP+T cells.
Differences in short-term outcomes after right and left sided colorectal resections for colorectal cancer may indicate a need for modification of ERAS protocol elements
Authors: Mantoo Surendra Kumar , Goh Serene 
Published: August 2022

Abstract
Purpose: Our study aims to compare postoperative bowel function recovery between standard right and left sided colorectal resections and explore the need to modify ERAS protocol for right sided colonic resections.Methods: data from a prospective database of elective right colonic resections (RCR) and left colorectal resections (LCR) between January 2016 to December 2019 was analyzed. Primary outcome was to compare return of bowel function and development of post-operative ileus between RCR and the LCR groups. Secondary outcomes were length of stay, post-operative complications, 30-day re-admission, and re-operation rates.Results: Of 193 eligible patients, 72 (37.3%) were in RCR group while 171 (62.7%) were in LCR group. Laparoscopic approach was used in (41/72) 56.9% of RCR group and (90/121) 74.4% in the LCR group. Time to escalation to low residue diet was 4.29 (1-18) days in RCR vs 2.89 (1-16) days in LCR group, p=0.001. Time to flatus was 3.07 (1-12) days in RCR group and 2.44 (1-6) days in LCR group, p=0.008. 21(29.2%) patients in RCR and 16 (13.2%) in LCR groups had postoperative ileus, p=0.007. Male sex and right colonic resections were found to be significant and independent risk factors for post-operative ileus after uni- and multivariate analysis. Conclusion: Right colonic resections are associated with delayed postoperative bowel function even after high compliance with ERAS protocol. Modification of ERAS protocol elements related to postoperative feeding and addition of new interventions may help to avoid delayed bowel function recovery.
Development and validation of a multiparametric semi-quantitative scoring system for the histopathological assessment of ischaemia severity in skeletal muscle.
Authors: Sanz-Nogués Clara , Creane Michael , Hynes Sean O. , Chen Xizhe , O’Brien Timothy 
Published: August 2022

Abstract
Background. Skeletal muscle is one of the most abundant and dynamic tissues of the body, with a strong regenerative capacity. Muscle injuries can occur as a result of a variety of events, including tissue ischaemia. Lower limb ischaemia occurs when there is an insufficient nutrient and oxygen supply, often caused by stenosis of the arteries due to atherosclerosis. The aim of this study was to develop and validate a multiparametric scoring tool for assessing ischaemia severity in skeletal muscle in a commonly used pre-clinical animal model.Methods. Tissue ischaemia was surgically induced in mice by ligation and excision of the femoral artery. Calf muscles were carefully dissected and prepared for histological analysis and scored for inflammation, fibrosis, necrosis, adipocyte (fat) infiltration and muscle fibre degeneration/regeneration. Tissue samples from calf muscles from 70 mice were scored by three independent blinded operators. Kendell’s coefficient of concordance (W) was used to measure agreement among the three observers and for intra-rater agreement. Spearman’s rank-order correlation was used to assess the association between the cumulative ischaemia severity score (cISS) and calf muscle weights.Results. Kendall’s W showed a very good agreement between the appraisers when scoring each individual histological feature: inflammation (W = 0.92 p-value 0.000), fibrosis (W = 0.94 p-value 0.000), necrosis (W = 0.77 p-value 0.000), adipocyte infiltration (W = 0.91 p-value 0.000) and fibre degeneration/regeneration (W = 0.86 p-value 0.000). Intra-rater agreement was also excellent (W = 0.94 or more, p-value 0.000). There was a statistically significant negative direct association between the level of muscle ischaemia damage (defined by the cISS) and the calf muscle weight (r= -0.86; R2adj.= 75.7% p-value 0.000).Conclusion. Here, we have developed and validated a new multiparametric, semi-quantitative scoring system for assessing skeletal muscle damage due to ischaemia, with excellent inter- and intra-rater reproducibility. This scoring system can be used for assessing treatment efficacy in a pre-clinical model of hindlimb ischaemia.
Effects of Forkhead Box D1 (FOXD1) on renal cell carcinoma
Authors: Wang Mengshan , Li Na , Qin Jiang , Hu Adam K 
Published: August 2022

AbstractRenal cell carcinoma is a common malignancy, and the treatment is not totally effective. Studies suggest that FOXD1 may be a critical determinant of tumor biology. Our study aims to identify the key genes and signaling to find the potential targets for the FOXD1-regulated renal cell carcinoma. In our study, we analyzed the RNA-seq data of Renal cell carcinoma (RCC) with the knockout of FOXD1 and identified the transcriptional landscapes. We then introduced the gene enrichment methods including KEGG and GO to further analyze the potential signaling pathways. Additionally, we constructed the PPI network and Reactome map to further identified the biological processes. We found that the PI3K − Akt signaling pathway and MAPK signaling pathway are the major signaling pathways in the FOXD1 knockout renal carcinoma cells. We identified the top ten interactive genes including TP53, ERBB2, CXCL8, CD44, MMP9, CCL2, ICAM1, HIF1A, FGF2, and CSF2. Our study provided new insights into the mechanisms for FOXD1-regulated renal cell carcinoma.
The regional case for disruptive energy policy: a civic energy perspective
Authors: McGovern Gerard 
Published: August 2022

Abstract
The paper deploys a regional actor perspective to detect policy enablers of and barriers to the uptake of civic energy. In common with other forms of regional, decentralized energy, the civic energy concept not only challenges historical energy business models but changes the energy agenda to prioritize community development and climate issues. Energy policy needs to react by shifting from purely market regulations to the promotion of shared community energy solutions. Both current energy structures and mainstream policy are unsuited to deliver on widely recognized energy transition and sustainable development goals. Switching from a centralized to a decentralized energy mode is viewed as a major re-structuring of a socio-technical regime. In a series of expert interviews, two disruptive policy approaches, creative destruction and experimentation, are analysed as to whether they can contribute to overcoming path dependency and carbon lock-in. Over and above dismantling existing administrative and legislative restrictions on civic energy uptake, experimentation is needed to develop new community partnerships that can host civic energy initiatives and mobilize untapped local energy resources. Whereas the fossil versus renewable energy policy debate seems to have been won, the shift from centralized to decentralized systems threatens existing power structures and remains highly contentious.
Economics and water allocation reform
Authors: Pérez-Blanco C. Dionisio 
Published: August 2022

Abstract
This chapter discusses major economic challenges and outlines key economic considerations in the design and implementation of water allocation reforms where agriculture is a major water use. It first examines key economic issues in water allocation reform, focusing on the challenges brought by the trade-offs between efficiency, social (distributive) justice, environmental sustainability and institutional reform, and the inherent uncertainties in managing these trade-offs, which are amplified by the complex nature of social-ecological systems. Based on this analysis, the chapter outlines a series of principles towards a water allocation reform that achieves sustainable, equitable and robust economic growth, and discusses the role of economic instruments in such reform. Finally, it identifies persistent research gaps towards delivering actionable science for informed water (re)allocations.