bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the application effect of family doctor contract service mode of "basic package + personalized package" in elderly hypertension management: Chinese experience
Authors: Du Qiujing , Ye Jiayi , Feng Jinhua , Gao Shilin , Li Ka 
Published: April 2022

Abstract
Background
At present, there is no relevant research on the application effect of "basic package + personalized package" family doctor contract service mode in hypertension management, therefore, this study intended to evaluate the application effect of this service model in the management of hypertension patients. We speculated that the implementation of this mode would improve the health outcomes of elderly patients with hypertension.
Methods
Patients with hypertension who participated in family doctor contract services in a community health service center in Chengdu, Southwest China from January 1, 2018 to December 31, 2020 were selected as the subjects of the study. According to the inclusion and exclusion criteria of the study, a total of 968 patients were included. The patients were divided into observation group and control group according to the type of service package they received. The primary outcomes included mean blood pressure (systolic, diastolic) and the rate of blood pressure control, Secondary outcomes included the level of cardiovascular disease risk and the level of self-management ability. All outcomes were assessed at baseline and 6 months after signing up.
Results
Of the 10,970 patients screened for eligibility, 968 (8.8%) were enrolled and divided into observation group (receiving "basic package + personalized package [hypertension]" service) (n = 403) and control group (receiving "basic package" service) (n = 565) according to the type of service package they received. Participants in the observation group compared with the control group showed better improvement in mean systolic blood pressure, the rate of blood pressure control, the level of cardiovascular disease risk, and the level of self-management ability at 6 months after signing up (Mean systolic blood pressure, P = 0.023; the rate of blood pressure control, P < 0.001; the level of cardiovascular disease risk, P < 0.001; the level of self-management ability, P < 0.001). There was no significant difference in mean diastolic blood pressure between the two groups (P = 0.735).
Conclusions
The family doctor contract service model of "basic package + personalized package (hypertension)" has a good application effect in the management of elderly hypertension, which can improve the average blood pressure, the rate of blood pressure control, the level of cardiovascular disease risk and the level of self-management ability of elderly hypertension patients.
New York State’s WAVE Method - Evaluation of a method for water quality monitoring by citizen scientists using benthic macroinvertebrates
Authors: Onion Alene M. , Smith Alexander J. , Duffy Brian T. 
Published: April 2022

Abstract
New York State Department of Environmental Conservation (NYSDEC) has developed a robust citizen science macroinvertebrate sampling method. The metric relies on the presence and not the absence of key macroinvertebrates and therefore is resistant to collection and sorting errors. It identifies unimpaired streams with high confidence (0.1% type 1 errors) and at a reasonable efficiency compared to NYSDEC’s multimetric index of biological integrity (54%). We rank remaining stream samples for further investigation using a calculated probability of impairment. This method is valuable as a tool for large monitoring programs with limited resources for quality assurance checks. The value of this method goes beyond data collection, however, as data of known quality is an effective communication tool between citizen scientists and state regulatory agencies and/or local decision makers.
Evaluating the Performance of Gadobutrol and Gadoterate Meglumine for Contrast-Enhanced Magnetic Resonance Angiography of the thoracic aorta at 1.5T
Authors: Pathrose Ashitha , Singer Alyssa , Cerne John Wence , Serhal Ali , Aouad Pascale , Blaisdell Julie , Avery Ryan , Markl Michael , Allen Bradley , Carr James C. 
Published: April 2022

Abstract
PURPOSE
The purpose of this study was to systematically compare the intra-individual image quality, signal parameters, and aortic dimensions between equal doses of gadobutrol (GB) and gadoterate meglumine (GM) in patients undergoing contrast-enhanced MRA of the thoracic aorta.
METHODS
33 patients (50 ± 12 years, 30M) with aortic disease who underwent GB-enhanced thoracic aortic MRA were prospectively recruited to undergo a GM-enhanced MRA within 8 weeks. MRAs were obtained after administration of 0.2mL/kg of 1.0M GB and 0.4mL/kg of 0.5M GM at flow rate of 2mL/s. Aortic luminal signal intensities (SI) and contrast ratios (CR) with respect to trapezius were measured at three regions. Aortic orthogonal diameters were measured at six regions. Overall image quality, vessel wall conspicuity, and artifacts were scored by two radiologists.
RESULTS
GB demonstrated significantly higher SI at all regions when compared to GM (AAo: 234 ± 97 vs. 116 ± 29, p < 0.001; Arch: 206 ± 92 vs. 99 ± 24, p < 0.001; DAo: 265 ± 132 vs. 102 ± 28, p < 0.001). CR at all regions (AAo: 3.0 ± 1.1 vs. 2.7 ± 1.0, p = 0.503; Arch: 2.7 ± 1.0 vs. 2.8 ± 1.1, p = 0.599; DAo: 3.3 ± 1.5 vs. 3.0 ± 1.1, p = 0.099) and aortic diameters at all regions did not show any significant difference between GB and GM. Overall image quality, vessel wall conspicuity, and artifact scores did not significantly differ between GB and GM.
CONCLUSION
This intra-individual study found that for contrast-enhanced MRA of the thoracic aorta, CR, aortic diameter measurements, overall image quality, vessel wall conspicuity, and artifact scores were comparable between equal doses of GB and GM. However, GB showed significantly higher aortic luminal SI when compared to GM.
Response and Recovery to COVID-19: Tibetan Refugees in Exile
Authors: Kaur Gurmeet 
Published: April 2022

Abstract
The study aimed to assess the impact of COVID-19 and mitigation strategies adopted by the Tibetan community. Narratives were collected from two settlements in January 2021-January’2022 using snowball sampling adhering to the research ethics involved. Respondents reported languishing socio-economic conditions because of partial/full lockdowns. Due to these confinements, the physical and mental health of the sentient being had also undergone unfavourable circumstances. At the same time, it tested an individual's ability to manage an adverse situation. In such precarious conditions, one among the other is religious traditions that bound human patience and tolerance.Nevertheless, Buddhist teachings disseminated by the various religious heads proved to be a boon for the Tibetan community regardless of their age. The study unveiled that a sense of collective good cultivated by Buddhist teachings had been helpful to protect the refugee population, both physically and mentally. Additionally, the theory of being a compassionate sentient being had been brought to praxis by the Tibetan community to contribute to the wellbeing of natives and their community. They have learned how to put calamity into an opportunity.
Whole-embryonic identification of maternal microchimeric cell types in mouse using single-cell RNA sequencing
Authors: Fujimoto Kana , Nakajima Akira , Hori Shohei , Tanaka Yumiko , Shirasaki Yoshitaka , Uemura Sotaro , Irie Naoki 
Published: April 2022

Abstract
Background: Even though the mother and the fetus of placental mammals are immunologically non-self with respect to one other, mutual exchange of small numbers of cells between them is known to occur. Maternal cells entering the fetus, called maternal microchimeric cells (MMc cells), are thought to be involved in different physiological phenomena, such as establishing immune tolerance, tissue repair, and the pathogenesis or deterioration of some inflammatory diseases and congenital malformations. While specific MMc cell types have been reported as associated with these phenomena, the contribution of MMc cells to these different outcomes remains unknown. As one possibility, we hypothesized that different embryos have differing repertoires of MMc cell types, leading to or biasing embryos toward different fates. To date, no studies have succeeded in identifying the MMc cell type repertoire of a single embryo. Accordingly, here, we isolated MMc cells from whole mouse embryos, determined their types, and analyzed their MMc cell type variability.Results: By combining our previously established, whole-embryonic MMc isolation method with single-cell RNA sequencing, we successfully estimated the cell type repertoires of MMc cells isolated from 26 mouse embryos. The majority of MMc cells were immune-related cells, such as myeloid cell and granulocyte. We also detected stem cell-like MMc cells expressing proliferation marker genes and terminally differentiated cells. As hypothesized, we noted statistically significant inter-individual variation in the proportion of immune-related cells in the different embryos.Conclusions: We here successfully estimated MMc cell types in individual whole mouse embryos. The proportion of immune-related cells significantly differed among the individual embryos, suggesting this as one of the potential mechanisms underlying the differing MMc-related physiological phenomena in offspring. These findings provide insight into cell-level epigenetics by maternal cells.
Antimicrobial Activity of Bacillus sp. K-9 Against Potato Scab and Its Genome-wide Analysis
Authors: Shuang Ma , Teng Wang , Ru Jiarong , Jiang Lili , Wang Yanjie , Wang Cong 
Published: April 2022

Abstract
We isolated a bacterial strain K-9, identified as a novel species of the genus Bacillus, from a potato tuber sample. The inhibition rate of the strain K-9 against potato scab (Streptomyces scabies) was 44.90%. The whole genome sequence of Bacillus sp. K-9 was obtained, and genomic analyses were performed. Complete genome sequence of strain K-9 was obtained through PacBio RSII and Illumina platforms. The size of genome is 3891530 bp, with estimated 3915 coding DNA sequences, GC content of 46.45%, 79 tRNA, and 10 rRNA. In addition, functional annotation of the strain K-9 genes was performed by GO, COG, KEGG, and CAZy analyses. There were 12 gene clusters for secondary metabolite synthesis in the genome of Bacillus sp. K-9. Except for the unknown metabolites of the T3PKS (third polyketone), terpene and lanthipeptide class-II, the other eight gene clusters are associated with the synthesis of secondary metabolites that are common in Bacillus and have antibacterial activity. Among them, the homologous alignment function types are mostly related to the synthesis of antibacterial active substances such as PKS (polyketone synthase), NRPS (non-ribosomal peptide synthase) and bacilysin. In the future, information derived from the thorough genomic analyses of this strain may be helpful in directed genetic modification of bacterial strains for production of antibacterial substances to control potato scab.
Isolation, molecular typing and antibiotic sensitivity profiling of enteric bacterial pathogen from chicken eggs
Authors: Kanhar Asif Raza , Phulpoto Irfan Ali , Ur-Rehman Saifu , Qazi Muneer Ahmed , Ghumro Waheed Ahmed , Hussain Syed Fiaz , Kanhar Ahmed Ali , Ujjan Javed Ahmed , Hussain Abdul 
Published: April 2022

Abstract
Food is a basic need of life; nevertheless its contamination due to pathogenic bacteria, especially antibiotic-resistant bacteria (ARB) in the poultry world, is a big threat to public health. In this study, enteric bacteria were isolated from chicken eggs and their antibiotic resistance against a panel of 18 different antibiotics belonging to 09 different antibiotic classes was determined. A total of 300 egg samples (i.e. 150 egg shells and 150 egg interiors) were collected and evaluated for bacterial growth. It was observed that 46% of the samples (n = 138) were positive for the growth of bacteria, while 54% of the samples (n = 162) did not show any bacterial growth in specified culture conditions. From those growth positive samples, three genera of Salmonella (from both the egg shell and the egg interior) and a genus of Enterobacter species, i.e. Enterobacter spp. (only from egg shells), were isolated using enrichment techniques and identified on the basis of cultural, morphological, biochemical, and 16S rRNA gene sequencing characteristics. The antibiotic sensitivity profiling results revealed that 50% of the isolated Enterobacter species were only resistant to amoxycillin, azomax (azithromycin), and fosfomycin, while 100% resistant to cephalothin, cefuroxime sodium, erythromycin, and vancomycin. On the other hand, 50% of the isolated species of Salmonella were resistant to amoxycillin, urixin, fosfomycin, and amoxycillin, whereas all Salmonella spp. were completely resistant to cephalothin, cefuroxime sodium, erythromycin, vancomycin, moxifloxacin, and azomax (azithromycin). The findings of the current study would increase the awareness of ordinary people to understand and overcome the associated risks associated with the utilization of contaminated eggs or other food products.
The Effect of Wearing a Powered Air Purifying Respirator Versus an N95 Mask on the Olfactory Function of Healthcare Workers
Authors: Xia Xiao , Liu Shixu , Xia Kun , Liu Yanyi , Zhang Jinzhi , Liu Xiaohong , Yao Yuhao , Li Guangxi 
Published: April 2022

Abstract
Background: With the COVID-19 epidemic, wearing a mask has become routine to prevent and control the virus's spread. Wearing masks for long periods can have various adverse effects on the human body, but little attention has been paid to the impact on olfaction. This study aimed to investigate whether wearing a mask affects healthcare workers' olfactory function and provide a reference for clinical olfactory research and mask wear regulations.Methods: We recruited fifty-six healthcare workers and randomly divided them into two groups, wearing a Powered Air Purifying Respirator (PAPR, experiment group, N=28) and an N95 mask (control group, N=28) for 8h. Olfactory discrimination tests and threshold tests were performed before and after wearing the masks. SPSS 26.0 software was used for the statistical analyses.Results: Current results suggested no statistical difference in the discrimination test scores between the PAPR Group (Z=-0.707 P =0.480) and N95 Group (Z=-0.828 P =0.408) before and after wearing the masks. The olfactory threshold test revealed a statistical difference in threshold scores before and after wearing the mask in the PAPR Group (Z= -2.595 P = 0.009) and N95 Group (Z= -2.120 P= 0.034). Both PAPRs and N95 masks reduce the sensitivity of healthcare workers to odors, with no significant difference between the two (χ2= 0.292，p = 0.589).Conclusion: Wearing a mask affects the healthcare workers’ olfaction, especially odor sensitivity. Healthcare workers wearing masks have a higher olfactory threshold than before， whether wearing PAPRs or N95 masks. More attention needs to be paid to the effect of masks on the olfactory function.
Comparing Oxidation of Aluminum by Oxygen and Ozone Using Reactive Force Field Molecular Dynamics Simulations
Authors: Saidinik Fateme , Behnejad Hassan 
Published: April 2022

Abstract
This work investigated the Al(100) surface oxidation simulations by O2 and O3 molecules at 400, 600, and 800 K temperatures with the Reactive Force Field (ReaxFF) method. One hundred ozone molecules and 150 oxygen molecules were placed in an empty space above the Al(100) surfaces in separate simulation boxes to compare this metal's behavior with them (gases incipient density of 0.47 g/cm3). We found further growth of the oxide layer in the case of ozone-aluminum. Subsequently, we surveyed the correlation between temperatures and the growth of alumina layers in the case of oxygen and ozone in separate sets through ReaxFF simulation to untangle the mechanism of oxidation kinetics of the Al(100) surface by ozone. Even though there are proven constraints on growing an oxide layer with ozone as well as oxygen, it’s possible to produce a thicker oxide layer at lower temperatures using ozone.
Expressed Emotion among families of female adolescents with nonsuicidal self- injury using a bidirectional approach
Authors: Tschan Taru , Pfeiffer Simone , Gutzweiler Raphael , In-Albon Tina 
Published: April 2022

Abstract
Background: Expressed Emotion refers to the extent to which close relatives express critical/hostile and/or emotionally overinvolved attitudes and feelings when speaking about a family member. High Expressed Emotion is a valuable predictor of clinical outcomes and is related to the presence of various mental disorders, including non-suicidal self-injury NSSI. Interpersonal factors have been shown to be relevant in initiating and in maintaining with non-suicidal self-injury (NSSI), as interpersonal difficulties are often reported as triggers for emotional dysregulation. The parental role in the treatment of NSSI still needs to be further investigated by focusing on their bidirectional relationship. This study uses a bidirectional approach for the assessment of Expressed Emotion in adolescents with non-suicidal self-injury (NSSI) and their mothers.Method: We examined Expressed Emotion levels of mother–daughter dyads among adolescents with NSSI, clinical controls (CCs), and nonclinical controls (NCs). The sample consisted of 70 female adolescents aged 12–20 years (M = 15.28 years, SD = 1.81; NSSI: n = 21, CC: n = 17, NC: n = 32) and 24 mothers aged 38–56 years (M = 46.47 years, SD = 4.61) using The Five-Minute Speech Sample (FMSS). Results: Adolescents with NSSI exhibited significantly more covert criticism and critical tone toward their mothers than CCs (d = 0.65, d = 1.10) and NCs (d = 1.30, d = 1.10). Conclusion: The findings highlight the importance of family-based interventions for the treatment of NSSI in order to enhance a positive relationship quality between parents and adolescents.
Liposomal stem cell extract formulation from Coffea canephora shows outstanding anti- inflammatory activity, increased tissue repair, neocollagenesis and neoangiogenesis
Authors: Guidoni Marcio , Júnior Antônio Domingos Sousa , Aragão Victor Paulo , Pereira Thiago Melo Costa , Santos Wedson Correa , Monteiro Flavio Cunha , Guimarães Marco Cesar Cunegundes , Fronza Marcio 
Published: April 2022

Abstract
Coffea canephora plant stem cells can have bioactive compounds with tissue repairing and anti-inflammatory action. This study aimed to develop a liposomal stem cell extract formulation from C. canephora (LSCECC) and to investigate its capacity to contribute to the dynamic mechanisms of tissue repair. The liposome was developed and characterized through the dynamic light scattering technique, atomic force microscopy, and transmission electron microscopy. The excisional full-thickness skin wound model was used and daily topically treated with the LSCECC formulation or vehicle control. On days 2, 7, 14, and 21 after wounding, five rats from each group were euthanized and the rates of wound closure and re-epithelialization were evaluated using biochemical and histological tests. LSCECC resulted in faster re-epithelialization exhibiting a significant reduction in wound area of ​​36.4, 42.4, and 87.5% after 7, 10, and 14 days, respectively, when compared to vehicle control. LSCECC treated wounds exhibited an increase in granular tissue and a proper inflammatory response mediated by the reduction of pro-inflammatory cytokines like TNF-α and IL-6 and an increase of IL-10. Furthermore, wounds treated with LSCECC showed an increase in the deposition and organization of collagen fibers at the wound site and improved scar tissue quality due to the increase in transforming growth factor-beta and vascular endothelial growth factor. Altogether, these data revealed that LSCECC may have remarkable applications in skincare cosmetic formulations to support healthy skin.
Canna x generalis irrigated with greywater in a nature-based solution
Authors: Menezes Caroline dos Santos , Takahashi Karen Midori , Bacchi Cláudia Gonçalves Vianna , de Lima Liana Baptista , Paulo Paula Loureiro 
Published: April 2022

Abstract
To achieve Goal 6 of the 2030 Agenda for Sustainable Development and provide water for all, water reuse is essential. We assessed the potential of treating greywater (LGw) while reusing it via irrigation of Canna x generalis, commonly used in nature-based solutions (NBS), with a view on plant development. The study was conducted at a mesocosm scale with factorial designs to test two substrates and four types of irrigation water. Stage 1 tested LGw, LGw with nutrients (LGw + N), and tap water with nutrients (TW + N). Stage 2 tested indoor and outdoor applications of TW (TWi and TWo, respectively) and LGw (LGwi and LGwo, respectively). All treatments resulted in reductions in total nitrogen and phosphate concentrations. The effluent turbidity, chemical oxygen demand, and surfactant contents of LGw + N and LGw decreased considerably after passing through the substrate. The results showed no statistical differences among the measured variables when LGw and TW received artificial fertilizers, which provided advantageous conditions for complete plant development, as evidenced by flowering. Conversely, compared with all other treatments, LGw treatment alone resulted in inferior development and exhibited some symptoms of nutritional deficiency, observed as reductions in dry biomass in the aboveground parts (range: 1.87–6.52 g) and belowground parts (10.03–36.19 g). Overall, Canna x generalis did not exhibit symptoms of toxicity and became fully developed, except for flowering, proving to be a robust species that is resistant to adverse environmental conditions, and is therefore recommended for cultivation in nature-based systems for landscaping integrated with LGw treatment and reuse.
COVID-19 vaccine effectiveness against SARS-CoV-2 infection in the United States prior to the Delta and Omicron-associated surges: a retrospective cohort study of repeat blood donors
Authors: Grebe Eduard , Yu Elaine A. , Bravo Marjorie D. , Welte Alex , Bruhn Roberta L. , Stone Mars , Green Valerie , Williamson Phillip C. , Feldstein Leora R. , Jones Jefferson M. , Busch Michael P. , Custer Brian 
Published: April 2022

Abstract
To inform public health policy, it is critical to monitor COVID-19 vaccine effectiveness (VE), including against acquiring infection. We estimated VE using a retrospective cohort study among repeat blood donors who donated during the first half of 2021, demonstrating a viable approach for monitoring of VE via serological surveillance. Using Poisson regression, we estimated overall VE was 88.8% (95% CI: 86.2–91.1), adjusted for demographic covariates and variable baseline risk. Time since first reporting vaccination, age, race-ethnicity, region, and calendar time were statistically significant predictors of incident infection. Studies of VE during periods of Delta and Omicron spread are underway.
Le Fort I osteotomy for removal of an odontoma from the maxillary sinus: a case report and literature review
Authors: Medeiros Yuri de Lima , Santos Lívia Marques dos , Resende Zarife Tirapani Adum , de Andrade Bruno Augusto Benevenuto , Marlière Daniel Amaral Alves 
Published: April 2022

Abstract
Odontoma is a common odontogenic tumour in the maxilla-mandibular region, but whose occurrence is rare within the maxillary sinus. The objective of the present study was to report a case of surgical removal of an extensive complex odontoma from within the maxillary sinus by using the Le Fort I osteotomy. A 22-year-old male patient presented an asymptomatic radiopaque lesion with well-defined margins in the right sinus cavity. The treatment consisted of surgical removal by using the Le Fort I osteotomy to facilitate access, full excision of the lesion and maintenance of bone integrity. The treatment was considered safe and effective as it allowed adequate access and low post-operative morbidity.