bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can Systematic Implementation Support Improve Program Fidelity by Improving Care Providers’ Perceptions of Implementation Factors? A Cluster Randomized Trial
Authors: Hartveit Miriam , Hovlid Einar , Øvretveit John , Assmus Jørg , Bond Gary , Joa Inge , Heiervang Kristin , Stensrud Bjørn , Høifødt Tordis Sørensen , Ruud Torleif 
Published: February 2022

Abstract
Background: Investigations of implementation factors are recommended to better understand and address inadequate implementation outcomes. Little is known about the relationship between implementation factors and outcome, especially in later phases of an implementation effort. The aims of this study were to assess the association between implementation success (measured by program fidelity) and care providers’ perceptions of implementation factors during an implementation process and to investigate whether these perceptions can be affected by systematic implementation support.Methods: Using a cluster-randomized design, mental health clinics were drawn to receive implementation support for one (intervention) and not for another (control) of four evidence-based practices. Program fidelity and care providers’ perceptions (Implementation Process Assessment Tool questionnaire) were scored for both intervention and control groups every sixth month for 18 months. Assessments and group differences were tested by means of descriptive statistics (mean, standard deviation and confidence interval) and linear mixed effect analysis.Results: Including 33 mental health centres or wards, we found care providers’ perceptions of a set of implementation factors to be associated with fidelity but not at baseline. After 18 months of implementation effort, fidelity and care providers’ perceptions were strongly correlated (B(95% CI)= .7 (.2, 1.1), p= .004). Care providers perceived implementation factors more positively when implementation support was provided than when it was not (t(140)=2.22, p=.028). Conclusions: Implementation support can facilitate positive perceptions among care providers, which is associated with higher program fidelity. To improve implementation success, we should pay more attention to how care providers constantly perceive implementation factors during all phases of the implementation effort. Further research is needed to investigate the validity of our findings in other settings and to improve our understanding of ongoing decision-making among care providers, i.e., the mechanisms of sustaining high fidelity of recommended practices. Trial registration: ClinicalTrials.gov Identifier: NCT03271242 (registration date: 05.09.2017).
WITHDRAWN: Germanium-Doped Coupled Ring-Core Fiber for Extremely Dispersive Orbital Angular Momentum Modes
Authors: Geng Wenpu , Fang Yuxi , Wang Yingning , Bao Changjing , Zhang Hao , Huang Hao , Ren Yongxiong , Pan Zhongqi , Yue Yang 
Published: February 2022

Abstract
A coupled ring-core fiber with extremely negative dispersion is proposed, simulated, and analyzed. Featured by two coaxial Ge-doped silica rings, this fiber can support highly dispersive orbital angular momentum (OAM) modes. The dependence of dispersion characteristics on the variable parameters of the fiber is theoretically and systematically investigated, including the geometry parameters and the mole fraction of GeO2. The order of the supported OAM modes in this fiber can be up to 100, achieving a highly negative dispersion reaching to -44,579 ps/(nm·km) at 1348.4 nm. The operation wavelength and the value of dispersion can be adjusted according to the need by selecting the fiber parameters. Furthermore, by adjusting the GeO2 concentration, the operation window of the OAM65,1 mode can be tuned in a much wider range from 1212.4 nm to 1666.2 nm.
Kawasaki Disease Concurrent with Visual Conversion Disorder in Child: A Case Report and Review of The Literature
Authors: Liu Junmei , Zhang Yingying , Zeng Huiqin , zhang jianjiang 
Published: February 2022

Abstract
Kawasaki disease (KD) is a self-limited vasculitis of medium- and small-sized vessels that occurs mainly in children between 5 months and 6 years of age. Conversion disorder is a type of somatoform disorder which is rare occurred in children under six years old. KD accompanied with conversion disorder has never been reported in the literature. We report a 4-year, 6-month-old female KD patient presenting with visual conversion disorder. She presented with classic KD symptoms, including high fever, rash, strawberry tongue and conjunctival and pharyngeal congestion. In addition, the patient displayed transient right vision loss during KD, while her ophthalmology and laboratory tests showed that there were no abnormalities that would result in vision loss; thus, psychogenic factors (conversion disorder) were considered after a simple test to confirm the functional vision loss of the right eye. The case could provides a differential diagnosis for clinical Kawasaki disease combined with vision disorder.
Construction of a prognostic nomogram for transarterial chemoembolization combined with 125I seed implantation for hepatocellular carcinoma with portal vein tumor thrombus: a multicenter retrospective study
Authors: Zhao Xiao-Hui , Zhao Lei , Zhao Yan , Li Hai-Liang , Li Zhen , Cao Guang-Shao , Li Hong-Le , Xu Shi-Jun , Hu Hong-Tao 
Published: February 2022

Abstract
Background: To investigate the efficacy of transhepatic arterial chemoembolization (TACE) combined with 125I seed implantation in patients with first-branch portal vein tumor thrombus (PVTT) hepatocellular carcinoma (HCC), establish a prognostic nomogram to determine the prognosis for individual patients.Methods: We retrospectively collected 102 cases of TACE combined with 125I seed implantation in our hospital as the primary cohort. The main observation index of the results of the study was the median overall survival (mOS). Univariate analysis and LASSO regression with cross-validation were used to screen the variables. The selected variables were included in the multivariate Cox survival regression model, and a prognostic nomogram was developed. The performance of the nomogram was assessed with respect to calibration, discrimination, and clinical usefulness. The internal and external verifications were evaluated.Results: The mOS of all patients was 13.5 months. The final determination was made using the Child-Pugh score, tumor burden, and PVTT types to establish a nomogram prediction model [C -index :0.740 (95% CI: 0.692-0.788), C-index: 0.732 through internal validation]. The model demonstrated good discrimination and calibration. Application of the nomogram to the external verification data still yielded good discrimination [C-index: 0.731(95% CI: 0.656–0.806)], and good calibration. The decision curve analysis demonstrated that the clinical validity of the model is clinically useful.Conclusion: TACE combined with 125I seed implantation indicated better long-term outcomes in patients with HCC combined with type II PVTT. This nomogram can be used to predict the prognostic survival time of such patients and help doctors make decisions in clinical practice.
Pseudogenization of the rhizobium-responsive EXOPOLYSACCHARIDE RECEPTOR in Parasponia is a rare event in nodulating plants
Authors: Dupin Simon , Klein Joël , Rutten Luuk , Huisman Rik , Geurts Rene 
Published: February 2022

Abstract
Background: Nodule symbiosis with diazotrophic Frankia or rhizobium occurs in plant species belonging to ten taxonomic lineages within the related orders Fabales, Fagales, Cucurbitales, and Rosales. Phylogenomic studies indicate that this nitrogen-fixing nodulation trait has a single evolutionary origin. In legume model plants, the molecular interaction between plant and rhizobium microsymbiont is mapped to a significant degree. A specific LysM-type receptor kinase, LjEPR3 in Lotus japonicus and MtLYK10 in Medicago truncatula, was found to act in a secondary identity-based mechanism, controlling intracellular rhizobium infection. Furthermore, LjEPR3 showed to bind surface exopolysaccharides of Mesorhizobium loti, the diazotrophic microsymbiont of L. japonicus. EPR3 orthologous genes are not unique to legumes. Surprisingly, however, its ortholog EXOPOLYSACCHARIDE RECEPTOR (EPR) is pseudogenized in Parasponia, the only lineage of non-legume plants that nodulate also with rhizobium.Results: Analysis of genome sequences showed that EPR3 orthologous genes are highly conserved in nodulating plants. We identified a conserved retrotransposon insertion in the EPR promoter region in three Parasponia species, which associates with defected transcriptional regulation of this gene. Subsequently, we studied the EPR gene of two Trema species as they represent the sister genus of Parasponia for which it is assumed it lost the nitrogen-fixing nodulation trait. Both Trema species possess apparently functional EPR genes that have a nodulation-specific expression profile when introduced into a Parasponia background. This indicates the EPR gene functioned in nodulation in the Parasponia-Trema ancestor.Conclusion: We conclude that nodule-specific expression of EPR3 orthologous genes is shared between the legume and Parasponia-Trema lineage, suggesting an ancestral function in the nitrogen-fixing nodulation trait. Pseudogenization of EPR in Parasponia is an exceptional case in nodulating plants. We speculate that this may have been instrumental to the microsymbiont switch -from Frankia to rhizobium- that has occurred in the Parasponia lineage and the evolution of a novel crack entry infection mechanism.
miR-100-5p Enhanced Cell Cycle-mediated Chemoresistance Through Modulating CTDSPL/pRB/E2F1 Signaling Pathway in Oxaliplatin-Resistant Colorectal Cancer
Authors: Chen Ming-Cheng , D Hema Sri , Singh Chaouhan Hitesh , Wang Tso-Fu , Li Chi-Cheng , Ho Tsung-Jung , Shibu Marthandam Asokan , Kuo Wei-Wen , Shih Tzu-Ching , Lin Yu-Jung , Huang Chih-Yang 
Published: February 2022

Abstract
Purpose: miR-100-5p has been found upregulated in Oxaliplatin-resistant LoVo colorectal cell lines, but its function still need further study. Here, we investigated its function in cell regulation chemoresistance in chemoresistant colorectal cancer cell lines.Methods: Microarray, quantitative polymerase chain reaction (qRT-PCR) and westernblot were used to analysis the level of miR-100-5p. The downstream candidate gen target of miR-100-5p, CTDSPL in Oxaliplatin resistant (OXR)-LoVo colorectal cell lines were screened using the different types of bioinformatics based miRNA target prediction data are TargetScan (http://www.targetscan.org/), miRanda (http://www.microrna.org/ and mirTar (http://mirtar.mbc.nctu.edu.tw/human/). CTDSPL, a phosphatase-like tumor suppressor level was detected by westernblot as well with cell cycle progression chemoresistance associated colorectal cancer cell CTDSPL/p-RB/E2F1. Immunofluorescence assay and westernblot showed the cell properties were altered between parental and Oxaliplatin resistant LoVo cell lines. Bioinformatic prediction, (http://alggen.1si.upc.es/cgi-bin/promo_v3) was used to extend the investigation of miR-100-5p upstream regulation through checking dissimilarity index with margin less or equal than 15%, FOXP3, trancription factor forkhead box as upstream target. Interaction between miR-100-5p and CTDSPL regulation was confirmed using mimic miR-100-5p overexpression and knockdown upstream target sh-RNA approach.Results: hsa-miR-100-5p level was found significantly upregulated in LoVo-OXR than parental LoVo cell line, resulted in downregulation CTDSPL as a major target of miR-100-5p. The downregulated of CTDSPL/p-RB/E2F1 promotes cell proliferation and cell cycle progression in LoVo OXR compared with LoVo parental cell lines. Moreover, among various upstream targets FOXP3 is an upstream regulator of miR-100-5p. In addition, mimic miR-100-5p overexpression downregulated CTDSPL/p-RB/E2F1 signaling pathway was reverted upon miR-100-5p inhibition in CRC cell lines.Conclusion: Collectively, miR-100-5p is chemoresistance inducer through modulation of CTDSPL/p-RB/E2F1 signaling pathway in CRC cell. This phenomenon might offer a therapeutic platform for overcoming chemoresistance in cancer treatment.
Endoscopic Features of Oxyntic Gland Adenoma and Gastric Adenocarcinoma of the Fundic Gland Type Differ Between Patients with and Without Helicobacter pylori Infection: A Retrospective Observational Study
Authors: Iwamuro Masaya , Kusumoto Chiaki , Nakagawa Masahiro , Matsueda Kazuhiro , Kobayashi Sayo , Yoshioka Masao , Inaba Tomoki , Toyokawa Tatsuya , Sakaguchi Chihiro , Tanaka Shouichi , Tanaka Takehiro , Okada Hiroyuki 
Published: February 2022

Abstract
Background
The endoscopic features of oxyntic gland adenoma and gastric adenocarcinoma of the fundic gland type have not been fully investigated in relation to Helicobacter pylori infection status. We compared the morphology, color, and location of these lesions between patients with and without H. pylori infection.
Methods
We retrospectively enrolled 165 patients (180 lesions) from 10 institutions. We divided the patients into the (i) Hp group (patients with current H. pylori infection [active gastritis, n = 13] and those with past infection [inactive gastritis, n = 76]) and (ii) uninfected group (H. pylori-uninfected patients, n = 52). We compared the clinical and endoscopic features of the two groups. We also performed an analysis between (i) lesions with atrophy of the surrounding gastric mucosa (atrophy group) and (ii) lesions without atrophy of the surrounding gastric mucosa (non-atrophy group).
Results
The average age was older in the Hp group than in the uninfected group (68.1 ± 8.1 vs. 63.4 ± 8.7 years, p < 0.01). Although the difference was not statistically significant (p = 0.09), multiple lesions were observed in 9 of 89 patients (10.1%) in the Hp group and in only 1 of 52 patients (1.9%) in the uninfected group. Meanwhile, significant differences were observed in the prevalence of lesions located in the gastric fornix or cardia (uninfected group: 67.3% vs. Hp group: 38.0%, p < 0.01), with an elevated morphology (80.0% vs. 56.0%, p < 0.01), with a subepithelial-like appearance (78.2% vs. 42.0%, p < 0.01), and with a color similar to that of the peripheral mucosa (43.6% vs. 25.0%, p = 0.02). The male-to-female ratio, lesion size, and presence or absence of vascular dilatation or black pigmentation on the surface were not different between the two groups. In the analysis comparing lesions with and without mucosal atrophy, the prevalence of multiple lesions was significantly higher (p = 0.02) in the atrophy group (5/25 patients, 20.0%) than in the non-atrophy group (7/141 patients, 5.0%).
Conclusions
The endoscopic features of oxyntic gland adenoma and gastric adenocarcinoma of the fundic gland type differ between patients with and without H. pylori infection.
Automatic Osteosarcoma Classification System Based Multilayer Grid XG Boost Architecture
Authors: B karthicsonia , Vanitha M. 
Published: February 2022

Abstract
Osteosarcoma is the most frequent primary malignant bone tumour. Computer-aided detection (CAD) and diagnosis are being used to enhance osteosarcoma detection and diagnosis . The use of machine learning and deep learning algorithms may save up surgeons' time while also improving patient outcomes. An enormous quantity of data must be fed into the classifier for it to become more accurate. Adapted to a public dataset of osteosarcoma histology pictures, a mix of machine and deep learning is used in this work to distinguish between necrotic and healthy tissue images. First, the dataset was preprocessed, and contour based threshold segmentation techniques are applied. Then, Stochastic linear embedding based Feature extraction is used for extracting the abnormal features. Finally, the proposed multilayer grid XG Boost classifier is trained on stained images in order to increase the output accuracy. The experimental findings indicate that the proposed classifier has the greatest accuracy of any illness classification approach currently available. Our finetuned model showed superior performance in identifying osteosarcoma malignancy using H and E stained pictures.
Monitoring Non-Parametric Profiles using Adaptive EWMA Control Chart
Authors: Yeganeh Ali , Abbasi Saddam A. , Shongwe Sandile C. 
Published: February 2022

Abstract
To monitor the quality of a process in statistical process control (SPC), considering a functional relationship between a dependent variable and one or more independent variables which is denoted as profile monitoring is becoming an increasingly common approach. Most of the studies in the SPC literature have dealt with cases in which the functional relationship has the same form in the in-control (IC) and out-of-control (OC) situation or considered parametric approaches. Non-parametric profiles, which have different relationship in OC conditions is also very common. This paper designs a novel control chart to monitor not only the regression parameters but also the variation of the profile in phase II applications, using an adaptive approach. Adaptive control charts adjust the final statistic with regard to information of previous samples and the proposed method considers the relative distance of the chart statistic to the control limits as a tendency index and provides some outcomes about the process condition. The results of Monte Carlo simulations show the superiority of the proposed monitoring scheme in comparison with the common non-parametric control chart.
Biosynthesis and characterization of CuO nanoparticles from plant of Adenathera pavonina linn bark and leaves.
Authors: OWOEYE TAIWO FELICIA , Akinlabu Kehinde Deborah , Emetere Moses , ADEBOYE Yinka John 
Published: February 2022

Abstract
Copper, a naturally occurring element, has medicinal potential and has recently gained in the textile industry. This paper seeks to chart the way forward on the commercial production of copper nanoparticles (Cu-NPs) for textile industry. The leaves and bark of Adenathera pavonina linn were used to synthesize copper nanoparticles (Cu-NPs) which are utilized in a range of applications as heterogeneous catalysts in textile industry. Copper nanoparticles were created using a chemical and natural method (Leaf extraction). In the absence of any reducing or stabilizing agents, the assynthesized samples were submitted to a range of analytical techniques in order to learn more about the physical properties of the materials. The radius of the Cu-NP from leave extract ranged from 56 nm – 89 µm with copper, carbon, and oxygen having a composition of 73.31%, 6.40% and 20.29% respectively. Likewise, the radius of Cu-NP from bark extract ranged from 56 nm – 293 µm with copper, oxygen, carbon, silicon, manganese, and aluminum having a composition of 65%, 24%, 1.22%, 4.20%, 3.10%, and 2.12% respectively. The impurities are largely from soil which the plant takes its nutrient. Carbon was found in both extract as it is expected in organic compounds in plants. The crystalline characterization shows that their exist a significant composition of Cu (I) species in both Cu-NP from the extracts that makes it highly relevant to the catalytic procedure in the textile industries. Each feature of the planes shows the copper atom represented as Cu-1 and Cu-2. Since Adenathera pavonina linn is in abundant in the tropics, its sustainability and low-cost production and processing are highly desired for textile industries.
A CDK-mediated phosphorylation switch of disordered protein condensation
Authors: Altelaar Maarten , Valverde Juan , Dubra Geronimo , Toorn Henk W.P. Van den , Mierlo Guido van , Vermeulen Michiel , Heck Albert , Elena-Real Carlos , Fournet Aurélie , Ghoul Emile Al , Chahar Dhanvantri , Haider Austin , Paloni Matteo , Constantinou Angelos , Barducci Alessandro , Ghosh Kingshuk , Sibille Nathalie , Bernadó Pau , Knipscheer Puck , Krasinska Liliana , Fisher Daniel 
Published: February 2022

Abstract
Cell cycle transitions arise from collective changes in protein phosphorylation states triggered by cyclin-dependent kinases (CDKs), but conceptual and mechanistic explanations for the abrupt cellular reorganisation that occurs upon mitotic entry are lacking. Specific interactions between distinct CDK-cyclin complexes and sequence motifs encoded in substrates might result in highly ordered phosphorylation1, while bistability in the mitotic CDK1 control network can trigger switch-like phosphorylation2. Yet the dynamics of mitotic phosphorylation has not been demonstrated in vivo, and the roles of most cell cycle-regulated phosphorylations are unclear. Here, we show evidence that switch-like phosphorylation of intrinsically disordered proteins (IDPs) by CDKs contributes to mitotic cellular reorganisation by controlling protein-protein interactions and phase separation. We studied protein phosphorylation in single Xenopus embryos throughout synchronous cell cycles, performed parallel assignment of cell cycle phases using egg extracts, and analysed dynamics of mitotic phosphorylation using quantitative targeted phosphoproteomics. This provided a high-resolution map of dynamic phosphosites from the egg to the 16-cell embryo and showed that mitotic phosphorylation occurs on entire protein complexes involved in diverse subcellular processes and is switch-like in vivo. Most cell cycle-regulated phosphosites occurred in CDK consensus motifs and located to intrinsically disordered regions. We found that substrates of CDKs and other cell cycle kinases are significantly more disordered than phosphoproteins in general, a principle conserved from yeast to humans, while around half are components of membraneless organelles (MLOs), whose assembly is thought to involve phase separation. Analytical modelling predicts modulation of homotypic IDP interactions by CDK-mediated phosphorylation, which was confirmed by biophysical and biochemical analysis of a model IDP, Ki-67. These results highlight the dynamic control of intrinsic disorder as a conserved hallmark of the cell cycle and suggest a mechanism for CDK-mediated mitotic cellular reorganisation.
Association between lipid profiles and the risk of incident early-onset vasomotor symptoms
Authors: Choi Hye Rin , Chang Yoosoo , Kim Yejin , Kang Jeonggyu , Kwon Ria , Lim Gayoung , Kim Kye-Hyun , Kim Hoon , Hong Yunsoo , Zhao Di , Cho Juhee , Guallar Eliseo , Park Hyun-Young , Ryu Seungho 
Published: February 2022

Abstract
Objectives: We investigated the associations between serum lipid profiles and risk of early-onset vasomotor symptoms (VMSs) in premenopausal women.Study design: This cohort study comprised 2,540 premenopausal women aged 42–52 years without VMSs at baseline (median follow-up: 4.4 years). Main outcome measures: VMSs, including hot flashes and night sweats, were assessed using the Menopause-Specific Quality of Life questionnaire (Korean version). Early-onset VMSs were defined as VMSs that occurred premenopause; moderate/severe VMSs were defined as a score of ≥ 3 points (range: 0 to 6, 6 being most bothersome). Parametric proportional hazard models were used to determine whether lipid levels affected VMS onset during the follow-up period. Results: Higher low-density lipoprotein (LDL) cholesterol levels were positively associated with increased risk of early-onset VMSs. Compared to the < 100 mg/dL LDL group, the multivariable-adjusted hazard ratios (HRs) with 95% confidence intervals (CIs) for incident VMSs were 1.19 (1.03–1.37) and 1.22 (1.04–1.42) in participants with LDL cholesterol levels of 100-129 mg/dL and ≥ 130 mg/dL, respectively (P for trend = 0.015). The multivariable-adjusted HR for incident moderate/severe VMSs was 1.40 (95% CI: 1.11–1.77) in participants with LDL ≥ 130 mg/dL, compared to those with LDL < 100 mg/dL. Meanwhile, triglycerides and total and high-density lipoprotein cholesterol levels were not significantly associated with early-onset VMSs risk in premenopausal women.Conclusions: Premenopausal women with high serum LDL cholesterol concentrations had a higher risk of incident early-onset VMSs. Further studies should confirm our findings and examine whether LDL-lowering interventions reduce the risk of early-onset VMSs among women during menopause transition.