bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gray Matter Volumetric Correlates of Attention Deficit and Hyperactivity Traits in Emerging Adolescents
Authors: Li Clara S. , Chen Yu , Ide Jaime S. 
Published: May 2022

AbstractPrevious research has demonstrated reduction in cortical and subcortical, including basal ganglia (BG), gray matter volumes (GMV) in individuals with attention deficit hyperactivity disorder (ADHD), a neurodevelopmental condition that is more prevalent in males than in females. However, the volumetric deficits vary across studies. Whether volumetric reductions are more significant in males than females; to what extent these neural markers are heritable and relate to cognitive dysfunction in ADHD remain unclear. To address these questions, we followed published routines and performed voxel-based morphometry analysis of a data set (n = 11,502; 5,464 girls, 9-10 years) curated from the Adolescent Brain Cognition Development project, a population-based study of typically developing children. Of the sample, 634 and 2,826 were identified as monozygotic twins (MZ) and dizygotic twins/siblings (DZ), respectively. In linear regressions, a cluster in the hypothalamus showed larger GMV, and bilateral caudate and putamen, lateral orbitofrontal and occipital cortex showed smaller GMVs, in correlation with higher ADHD scores in girls and boys combined. When examined separately, boys relative to girls showed more widespread (including BG) and stronger associations between GMV deficits and ADHD scores. ADHD traits and the volumetric correlates demonstrated heritability estimates (a2) between 0.59 and 0.79, replicating prior findings of the genetic basis of ADHD. Further, ADHD traits and the volumetric correlates (except for the hypothalamus) were each negatively and positively correlated with N-back performance. Together, these findings confirm volumetric deficits in children with more prominent ADHD traits. Highly heritable in both girls and boys and potentially more significant in boys than in girls, the structural deficits underlie diminished capacity in working memory and potentially other cognitive deficits in ADHD.
Imaging unlabeled axons in the mouse retina by second harmonic generation
Authors: Meah Arafat , Boodram Vinessia , Lim Hyungsik 
Published: May 2022

AbstractWe describe a novel microscopy for studying the network of axons in the unlabeled fresh wholemount retina. The intrinsic radiation of second harmonic generation (SHG) was utilized to visualize single axons of all major retinal neurons, i.e., photoreceptors, horizontal cells, bipolar cells, amacrine cells, and the retinal ganglion cells. The cell types of SHG+ axons were determined using transgenic GFP/YFP mice. New findings were obtained with retinal SHG imaging: Müller cells do not maintain uniformly polarized microtubules in the processes; SHG+ axons of bipolar cells terminate in the inner plexiform layer (IPL) in a subtype-specific manner; a subset of amacrine cells, presumably the axon-bearing types, emits SHG; and the axon-like neurites of amacrine cells provide a cytoskeletal scaffolding for the IPL stratification. To demonstrate the utility, retinal SHG imaging was applied for testing whether the inner retina is preserved in glaucoma, using DBA/2 mice as a model of glaucoma and DBA/2-Gpnmb+ as the non-glaucomatous control. It was found that the morphology of the inner retina was largely intact in glaucoma and the pre-synaptic compartments to the retinal ganglion cells were uncompromised. It proves retinal SHG imaging as a promising technology for studying the physiological and diseased retina in 3D.
Modification-Dependent Restriction Endonuclease-based sequencing method (EcoWI-seq) maps the genome-wide landscape of phosphorothioate modification at base resolution
Authors: Yang Weiwei , Fomenkov Alexey , Heiter Dan , Xu Shuang-yong , Ettwiller Laurence 
Published: May 2022

AbstractPhosphorothioation (PT), in which a non-bridging oxygen is replaced by a sulfur, is one of the rare modifications discovered in bacteria and archaea that occurs on the sugar-phosphate backbone as opposed to the nucleobase moiety of DNA. While PT modification is widespread in the prokaryotic kingdom, how PT modifications are distributed in the genomes and their exact roles in the cell remain to be defined. In this study, we developed a simple and convenient technique called EcoWI-seq based on a modification-dependent restriction endonuclease to identify genomic positions of PT modifications. EcoWI-seq shows similar performance than other PT modification detection techniques and additionally, is easily scalable while requiring little starting material. As a proof of principle, we applied EcoWI-seq to map at base resolution the PT modifications in the genomes of both the Salmonella enterica cerro 87 and E. coli expressing the dnd+ gene cluster. Specifically, we address whether the partial establishment of modified PT positions is a stochastic or deterministic process. EcoWI-seq reveals a systematic usage of the same subset of target sites in clones for which the PT modification has been independently established.
Peripheral sensory neuron CB2 cannabinoid receptors are necessary for both CB2-mediated antinociceptive efficacy and sparing of morphine tolerance in a mouse model of neuropathic pain
Authors: Carey Lawrence M. , Xu Zhili , Rajic Gabriela , Makriyannis Alexandros , Romero Julian , Hillard Cecilia , Mackie Ken , Hohmann Andrea G. 
Published: May 2022

AbstractPainful peripheral neuropathy is the most common neurological complication associated with human immune deficiency virus (HIV) infection. Currently available treatments fail to provide adequate symptom relief, indicating the need for novel treatment strategies. To address this gap in knowledge, we characterized the impact of cannabinoid CB2 agonists, which lack psychoactivity associated with central CB1 activation, on antiretroviral-induced neuropathic nociception and identified cell types expressing CB2 that mediate the antinociceptive efficacy of CB2 agonists. Two structurally distinct CB2 agonists (AM1710 and LY2828360) alleviated antiretroviral-induced neuropathic pain, benefits which were absent in CB2 knockout mice. Conditional deletion of CB2 from peripheral sensory neurons eliminated the antinociceptive efficacy of CB2 agonists. We also asked whether LY2828360 treatment could reverse established morphine tolerance in the ddC-induced neuropathy model and whether CB2 expression on peripheral sensory neurons is necessary for sparing of morphine tolerance by LY2828360. The present studies suggest that CB2 activation may alleviate HIV-associated antiretroviral neuropathy and identify a previously unreported mechanism through which CB2 activation produces antinociceptive efficacy. Our results also provide the first evidence that a CB2 agonist can reverse established morphine tolerance and demonstrate that CB2 localized to peripheral sensory neurons mediates the opioid tolerance sparing efficacy of CB2 agonists.
A live attenuated vaccine confers superior mucosal and systemic immunity to SARS-CoV-2 variants
Authors: Nouailles Geraldine , Adler Julia M. , Pennitz Peter , Peidli Stefan , Alves Gustavo Teixeira , Baumgart Morris , Bushe Judith , Voss Anne , Langenhagen Alina , Pott Fabian , Kazmierski Julia , Goekeri Cengiz , Simmons Szandor , Xing Na , Langner Christine , Vidal Ricardo Martin , Abdelgawad Azza , Herwig Susanne , Cichon Günter , Niemeyer Daniela , Drosten Christian , Goffinet Christine , Landthaler Markus , Blüthgen Nils , Wu Haibo , Witzenrath Martin , Gruber Achim D. , Praktiknjo Samantha D. , Osterrieder Nikolaus , Wyler Emanuel , Kunec Dusan , Trimpert Jakob 
Published: May 2022

AbstractVaccines are a cornerstone in COVID-19 pandemic management. Here, we compare immune responses to and preclinical efficacy of the mRNA vaccine BNT162b2, an adenovirus-vectored spike vaccine, and the live-attenuated-virus vaccine candidate sCPD9 after single and double vaccination in Syrian hamsters. All regimens containing sCPD9 showed superior efficacy. The robust immunity elicited by sCPD9 was evident in a wide range of immune parameters after challenge with heterologous SARS-CoV-2 including rapid viral clearance, reduced tissue damage, fast differentiation of pre-plasmablasts, strong systemic and mucosal humoral responses, and rapid recall of memory T cells from lung tissue. Our results demonstrate that use of live-attenuated vaccines may offer advantages over available COVID-19 vaccines, specifically when applied as booster, and may provide a solution for containment of the COVID-19 pandemic.
Channel-independent function of UNC-9/INX in spatial arrangement of GABAergic synapses in C. elegans
Authors: Hendi Ardalan , Niu Longgang , Snow Andrew , Ikegami Richard , Wang Zhao-Wen , Mizumoto Kota 
Published: May 2022

AbstractPrecise synaptic connection of neurons with their targets is essential for the proper functioning of the nervous system. A plethora of signaling pathways act in concert to mediate the precise spatial arrangement of synaptic connections. Here we show a novel role for a gap junction protein in controlling tiled synaptic arrangement in the GABAergic motor neurons in C. elegans, in which their axons and synapses overlap minimally with their neighboring neurons within the same class. We found that while EGL-20/Wnt controls axonal tiling, their presynaptic tiling is mediated by a gap junction protein UNC-9/Innexin, that is localized at the presynaptic tiling border between neighboring DD neurons. Strikingly, the gap junction channel activity of UNC-9 is dispensable for its function in controlling tiled presynaptic patterning. While gap junctions are crucial for the proper functioning of the nervous system as channels, our finding uncovered the novel channel-independent role of UNC-9 in synapse patterning.
Interactive Neurorobotics: Behavioral and Neural Dynamics of Agent Interactions
Authors: Leonardis Eric , Breston Leo , Lucero-Moore Rhiannon , Sena Leigh , Kohli Raunit , Schuster Luisa , Barton-Gluzman Lacha , Quinn Laleh K. , Wiles Janet , Chiba Andrea 
Published: May 2022

AbstractInteractive neurorobotics is a subfield which characterizes brain responses evoked during interaction with a robot, and their relationship with the behavioral responses. Gathering rich neural and behavioral data from humans or animals responding to agents can act as a scaffold for the design process of future social robots. The goals of this research can be broadly broken down into two categories. The first, seeks to directly study how organisms respond to artificial agents in contrast to biological or inanimate ones. The second, uses the novel affordances of the robotic platforms to investigate complex phenomena, such as responses to multisensory stimuli during minimally structured interactions, that would be difficult to capture with classical experimental setups. Here we argue that to realize the full potential of the approach, both goals must be integrated through methodological design that is informed by a deep understanding of the model system, as well as engineering and analytical considerations. We then propose a general framework for such experiments that emphasizes naturalistic interactions combined with multimodal observations and complementary analysis pipelines that are necessary to render a holistic picture of the data for the purpose of informing robotic design principles. Finally, we demonstrate this approach with an exemplar rat-robot social interaction task which included simultaneous multi-agent tracking and neural recordings.
Metabolic plasticity of serine metabolism is crucial for cGAS/STING-signalling and innate immune response to viral infections in the gut
Authors: Becker Björn , Wottawa Felix , Bakr Mohamed , Koncina Eric , Mayr Lisa , Kugler Julia , Yang Guang , Windross Samuel J , Neises Laura , Mishra Neha , Harris Danielle , Tran Florian , Welz Lina , Schwärzler Julian , Bánki Zoltán , Stengel Stephanie T , Ito Go , Krötz Christina , Coleman Olivia I , Jaeger Christian , Haller Dirk , Paludan Søren R , Blumberg Richard , Kaser Arthur , Cicin-Sain Luka , Schreiber Stefan , Adolph Timon E. , Letellier Elisabeth , Rosenstiel Philip , Meiser Johannes , Aden Konrad 
Published: May 2022

AbstractInflammatory bowel diseases (IBD) are characterized by chronic relapsing inflammation of the gastrointestinal tract. While the molecular causality between endoplasmic reticulum (ER) stress and intestinal inflammation is widely accepted, the metabolic consequences of chronic ER-stress on the pathophysiology of IBD remain unclear. By usingin vitro,ex vivo,in vivomouse models and patient datasets, we identified a distinct polarisation of the mitochondrial one-carbon (1C) metabolism and a fine-tuning of the amino acid uptake in intestinal epithelial cells tailored to support GSH and NADPH metabolism upon chronic ER-stress. This metabolic phenotype strongly correlates with IBD severity and therapy-response. Mechanistically, we uncover that both chronic ER-stress and serine limitation disrupt cGAS/STING-signalling, impairing the epithelial response against viral and bacterial infection, fuelling experimental enteritis. Consequently, antioxidant treatment restores STING function and virus control. Collectively, our data highlight the importance of the plasticity of serine metabolism to allow proper cGAS/STING-signalling and innate immune responses upon chronic inflammation in the gut.
Anthrax intoxication reveals that ER-Golgi membrane contact sites control the formation of plasma membrane lipid nanodomains
Authors: Anwar Muhammad U. , Sergeeva Oksana A. , Abrami Laurence , Mesquita Francisco , Lukonin Ilya , Amen Triana , Chuat Audrey , Capolupo Laura , Liberali Prisca , D’Angelo Giovanni , van der Goot F. Gisou 
Published: May 2022

AbstractTo promote infections, pathogens exploit host cell machineries including structural elements of the plasma membrane. Studying these interactions and identifying involved molecular players is an ideal way to gain insights into the fundamental biology of the host cell. Here, using the anthrax toxin, we screened a 1500-gene library of regulatory, cell surface, and membrane trafficking genes for their involvement in the intoxication process. We found that the ER–Golgi-localized proteins TMED2 and TMED10 are required for toxin oligomerization at the cell surface, an essential step for anthrax intoxication that depends on localization to cholesterol-rich lipid nanodomains. Further biochemical, morphological and mechanistic analyses showed that TMED2 and TMED10 are essential components of a multiprotein supercomplex that operates exchange of both cholesterol and ceramides at ER-Golgi membrane contact sites. Overall, this study of anthrax intoxication led to the discovery that lipid compositional remodelling at ER-Golgi interfaces fully controls the formation of functional membrane nanodomains at the cell surface.
Robust optimal design of low frequency metamaterials considering the fabrication imperfections and parameter fluctuations
Authors: Li Yiying , Sun Dun , Yang Shiyou 
Published: May 2022

Purpose
The purpose of this paper is to develop a robust optimization methodology for metamaterial (MM) unit designs to minimize the effect of manufacturing and operational uncertainties.

Design/methodology/approach
A new robustness quantification function, applicable to both convex and nonconvex relationships between the mean and the standard deviation, is introduced. A distance-based local radial basis function network surrogate model is proposed to substitute the global radial basis function network to reduce the heavy computational cost without any scarification on the solution accuracy.

Findings
The optimized results of a prototype MM unit demonstrate the feasibility and merit of the proposed methodology. The proposed methodology outperforms the existing ones in both performance and robust parameters in the design of a prototype MM unit.

Originality/value
It provides a robust optimization methodology for MM units when considering the imperfections in fabrications and fluctuations in operation and environment conditions in engineering applications.
Unpacking the context of value for money assessment in global markets: a procurement option framework for public-private partnerships
Authors: Zhao Jianfeng , Thurairajah Niraj , Greenwood David , Liu Henry , Yuan Jingfeng 
Published: May 2022

PurposeThe unprecedented SARS-CoV-2 (COVID-19) pandemic has further constrained the budgets of governments worldwide for delivering their much-needed infrastructure. Consequently, public-private partnerships (PPPs), with the private sector's investment and ingenuity, would appear to be an increasingly popular alternative. Value for money (VfM) has become the major criterion for evaluating PPPs against the traditional public sector procurement and, however, is plagued with controversy. Hence, it is important that governments compare and contrast their practice with similar and disparate bodies to engender best practice. This paper, therefore, aims to understand governments' assessment context and provide a cross-continental comparison of their VfM assessment.Design/methodology/approachFaced with different domestic contexts (e.g. aging infrastructure, population growth, and competing demands on finance), governments tend to place different emphases when undertaking the VfM assessment. In line with the theory of boundary spanning, a cross-continental comparison is conducted between three of the most noticeable PPP markets (i.e. the United Kingdom, Australia and China) about their VfM assessment. The institutional level is interpreted by a social, economic and political framework, and the methodological level is elucidated through a qualitative and quantitative VfM assessment.FindingsThere are individual institutional characteristics that have shaped the way each country assesses VfM. For the methodological level, we identify that: (1) these global markets use a public sector comparator as the benchmark in VfM assessment; (2) ambiguous qualitative assessment is conducted only against PPPs to strengthen their policy development; (3) Australia's priority is in service provision whereas that of the UK and China is project finance and production; and (4) all markets are seeking an amelioration of existing controversial VfM assessments so that purported VfM relates to project lifecycles. As such, an option framework is proposed to make headway towards a sensible selection of infrastructure procurement approaches in the post COVID-19 era.Originality/valueThis study addresses a current void of enhancing the decision-making process for using PPPs within today's changing environment and then opens up an avenue for future empirical research to examine the option framework and ensuing VfM decisions. Practically, it presents a holistic VfM landscape for public sector procurers that aim to engage with PPPs for their infrastructure interventions.
Do domestic market characteristics influence firms' export intensity?
Authors: Forte Rosa Portela , Carvalho Sérgio 
Published: May 2022

PurposeThe purpose of this study is to analyze the influence of the firms' external environment on their export intensity. More specifically, it assesses whether domestic market characteristics such as domestic demand and general export environment related to tradability across borders affect firms' export intensity.Design/methodology/approachThe authors use a sample of 29,266 firms from nine European countries, for the period of 2010–2016, and test several estimation methods (random effects models, Tobit models, and Heckman's selection models).FindingsResults show that external factors such as domestic demand and ease of trade across borders are important determinants of firms' export intensity. Moreover, results reveal that firm's internal characteristics such as age, size and productivity also play an import role.Originality/valueStudies about the influence of the firms' external environment on firms' export intensity are scarce because most of them are confined to a single country context. In this way, the present study contributes to the body of knowledge on the influence that external factors can have on firms' export performance by analyzing firms from nine European countries, which has important policy implications.
Effects of cognitive absorption on continuous use intention of AI-driven recommender systems in e-commerce
Authors: Acharya Nirmal , Sassenberg Anne-Marie , Soar Jeffrey 
Published: May 2022

Purpose
The applications of artificial intelligence (AI), natural language processing and machine learning in e-commerce are growing. Recommender systems (RSs) are interaction-based technologies based on AI that can offer recommendations for products for use or of interest to a potential consumer. Curiosity, focused immersion and temporal dissociation are often treated as the dimensions of cognitive absorption, so exploring them separately can provide valuable insights into their dynamics. The paper aims to determine the effect of the cognitive absorption dimensions namely focused immersion, temporal dissociation and curiosity independently on RSs continuous use intention.

Design/methodology/approach
A quantitative research design was used to explore the effect of dimensions of cognitive absorption on AI-driven RSs continuous use intention in e-commerce. Data were gathered from 452 active users of Amazon through an online cross-sectional survey and were analysed using partial least squares structural equation modelling.

Findings
The findings indicated that curiosity and focused immersion directly affect RSs continuous use intention, but temporal dissociation does not affect RSs continuous use intention.

Originality/value
The current research focused on Amazon’s RSs that use AI and machine learning techniques. The research aimed to empirically explore the effects of the dimensions of cognitive absorption separately on AI-driven RSs continuous use intention in e-commerce. This research may be of interest to executives working in both public and private industries to better harness the potential of recommendations driven by AI to maximize RSs’ reuse and to enhance customer loyalty.