bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 spike proteins uptake mediated by lipid raft ganglioside GM1 in human cerebrovascular cells
Authors: McQuaid Conor , Solorzano Alexander , Dickerson Ian , Deane Rashid 
Published: March 2022

AbstractWhile there is clinical evidence of neurological manifestation in coronavirus disease-19, it’s unclear whether this is due to differential severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uptake from blood by cells of the cerebrovasculature. SARS-CoV-2 and its spike protein (SP) interact with the endothelium but the roles of extracellular peptidase domain on angiotensin converting enzyme 2 receptors (ACE2) and ACE2 independent pathways (such as glycans) are not fully elucidated. In addition, for SARS-CoV-2 to enter the brain parenchyma from blood it has to cross several cell types, including the endothelium, pericytes and vascular smooth muscle. Since SARS-CoV-2 interacts with host cells via it SP at the entry point of it life cycle, we used fluorescently labelled SP (SP-555) (wild type and mutants) to model viral behaviour, in vitro, for these cell types (endothelial, pericytes and vascular smooth muscle) to explore pathways of viral entry into brain from blood. There was differential SP uptake by these cell types. The endothelial cells had the least uptake, which may limit SP uptake into brain from blood. Uptake was mediated by ACE2, but it was dependent on SP interaction with ganglioside GM1 in the lipid raft. Mutation sites, N501Yand E484K and D614G, as seen in variants of interest, were differentially taken up by these cell types. There was greater uptake but neutralization with anti-ACE2 and anti-GM1antibodies was less effective. Our data suggested that GM1/lipid raft is an important entry point of SARS-CoV-2 into these cells since inhibition of SP uptake with both anti-ACE2 and anti-GM1 together was similar to that with only anti-GM1, and both ACE2 and GM1 are within the lipid raft region of plasma membrane. Thus, GM1 is a potential SARS-CoV-2 and therapeutic target at the cerebrovasculature.
In-silico method for predicting infectious strains of Influenza A virus from its genome and protein sequences
Authors: Roy Trinita , Sharma Khushal , Dhall Anjali , Patiyal Sumeet , Raghava Gajendra P. S. 
Published: March 2022

AbstractInfluenza A is a contagious viral disease responsible for four pandemics in the past and a major public health concern. Being zoonotic in nature, the virus can cross the species barrier and transmit from wild aquatic bird reservoirs to humans via intermediate hosts. Virus gradually undergoes host adaptive mutations in their genome and proteins, resulting in different strain s/vari ants which might spread virus from avians/mammals to humans. In this study, we have developed an in-silico models to identify infectious strains of Influenza A virus, which has the potential of getting transmitted to humans, from its whole genome/proteins. Firstly, machine learning based models were developed for predicting infectious strains using composition of 15 proteins of virus. Random Forest based model of protein Hemagglutinin, achieved maximum AUC 0.98 on validation data using dipeptide composition. Secondly, we obtained maximum AUC of 0.99 on validation dataset using one-hot-encoding features of each protein of virus. Thirdly, models build on DNA composition of whole genome of Influenza A, achieved maximum AUC 0.98 on validation dataset. Finally, a web-based service, named “FluSPred”(https://webs.iiitd.edu.in/raghava/fluspred/) has been developed which incorporate best 16 models (15 proteins and one based on genome) for prediction of infectious strains of virus. In addition, we provided standalone software for the prediction and scanning of infectious strains at large-scale (e.g., metagenomics) from genomic/proteomic data. We anticipate this tool will help researchers in prioritize high-risk viral strains of novel influenza virus possesses the capability to spread human to human, thereby being useful for pandemic preparedness and disease surveillance.Key PointsInfluenza A is a contagious viral disease responsible for four pandemics.Virus can cross species barrier and infect human beings.In silico models developed for predicting human infectious strains of virus.Models developed were build using 15 proteins and whole genome datasets.Webserver and standalone package for predicting and scanning of high-risk viral strains.
Liguleless narrow and narrow odd dwarf act in overlapping pathways to regulate maize development & physiology
Authors: Abraham-Juárez María Jazmín , Busche Michael , Anderson Alyssa A. , Lunde China , Winders Jeremy , Christensen Shawn A. , Hunter Charles T. , Hake Sarah , Brunkard Jacob O. 
Published: March 2022

ABSTRACTnarrow odd dwarf (nod) and Liguleless narrow (Lgn) are pleiotropic maize mutants that both encode plasma membrane proteins, cause similar developmental patterning defects, and constitutively induce stress signaling pathways. To investigate how these mutants coordinate maize development and physiology, we screened for protein interactors of NOD by affinity purification. LGN was identified by this screen as a strong candidate interactor, and we confirmed the NOD-LGN molecular interaction through orthogonal experiments. We further demonstrated that LGN, a receptor-like kinase, can phosphorylate NOD in vitro, hinting that they could act in intersecting signal transduction pathways. To test this hypothesis, we generated Lgn-R;nod mutants in two backgrounds (B73 and A619) and found that these mutations enhance each other, causing more severe developmental defects than either single mutation on its own, with phenotypes including very narrow leaves, increased tillering, and failure of the main shoot. Transcriptomic and metabolomic analyses of the single and double mutants in the two genetic backgrounds revealed widespread induction of pathogen defense genes and a shift in resource allocation away from primary metabolism in favor of specialized metabolism. These effects were similar in each single mutant and heightened in the double mutant, leading us to conclude that NOD and LGN act cumulatively in overlapping signaling pathways to coordinate growth-defense tradeoffs in maize.
Ultrabithorax modifies a regulatory network of genes essential for butterfly eyespot development in a wing sector-specific manner
Authors: Matsuoka Yuji , Monteiro Antónia 
Published: March 2022

SummaryNymphalid butterfly species often have a different number of eyespots in forewings and hindwings but how the hindwing identity gene, Ultrabithorax (Ubx), drives this asymmetry, is not fully understood. It is also unclear why eyespot serial homologs originated initially only in hindwings. To address these questions, we examined a three-gene regulatory network (GRN) for eyespot development in the hindwings of Bicyclus anynana butterflies, and compared it to the same network previously described for forewings. We also examined how Ubx interacted with each of these three eyespot-essential genes. We found similar genetic interactions between the three genes in fore and hindwings, but we discovered three regulatory differences: Antennapedia (Antp) merely enhances spalt (sal) expression in the eyespot foci in hindwings but is not essential for sal activation, as in forewings; Ubx up- regulates Antp in all hindwing eyespot foci but represses Antp outside these wing regions and; Ubx is regulating sal expression in a wing-sector specific manner, i.e., it does not affect sal expression in the wing sectors that contain eyespots in both fore and hindwings, but it positively regulates sal expression in the sectors that have hindwing-specific eyespots. These results suggest that Ubx, or its downstream targets, might have paid a role in the origin of eyespots, restricted initially to hindwings, via the positive regulation of sal, an essential gene for eyespot development. We propose that Antp got co-opted into the eyespot GRN at a later stage by coming under Ubx regulation. This Hox gene redundancy, together with a novel positive feedback loop between sal and Antp, might have allowed Antp to functionally replace Ubx in forewings and lead to the origin of forewing eyespots. Outside the eyespot focal regions, we discovered that Ubx is up-regulated by Distal-less along the wing margin. We propose a model for how the regulatory connections between these genes might have evolved to produce wing- and sector-specific variation in eyespot number.
Complete chloroplast genomes and phylogeny in three Euterpe palms (E. edulis, E. oleraceae and E. precatoria) from different Brazilian biomes
Authors: Francisconi Ana Flávia , dos Santos Luiz Augusto Cauz , Morales Marroquín Jonathan Andre , van den Berg Cássio , Alves-Pereira Alessandro , Delmondes de Alencar Luciano , Picanço-Rodrigues Doriane , Zanello Cesar Augusto , Ferreira Costa Marcones , Gomes Lopes Maria Teresa , Veasey Elizabeth Ann , Zucchi Maria Imaculada 
Published: March 2022

AbstractThe Brazilian palm fruits and hearts-of-palm of Euterpe edulis, E. oleracea and E. precatoria are an important source for agro-industrial production, but overexploitation requires conservation strategies to maintain genetic diversity. Chloroplast genomes have conserved sequences, which are useful to explore evolutionary questions. Besides the plastid DNA, genome skimming allows the identification of other genomic resources, such as single nucleotide polymorphisms (SNPs), providing information about the genetic diversity of species. We sequenced the chloroplast genome and identified gene content in the three Euterpe species. We performed comparative analyses, described the polymorphisms among the chloroplast genome sequences (repeats, indels and SNPs) and performed a phylogenomic inference based on 55 palm species chloroplast genomes. Finally, using the remaining data from genome skimming, the nuclear and mitochondrial reads, we identified SNPs and estimated the genetic diversity among these Euterpe species. The Euterpe chloroplast genomes varied from 159,232 to 159,275 bp and presented a conserved quadripartite structure with high synteny with other palms. In a pairwise comparison, we found a greater number of insertions/deletions (indels = 93 and 103) and SNPs (284 and 254) between E. edulis/E. oleracea and E. edulis/E. precatoria when compared to E. oleracea/E. precatoria (58 indels and 114 SNPs). Also, the phylogeny indicated a closer relationship between E. oleracea/E. precatoria. The nuclear and mitochondrial genome analyses identified 1,077 SNPs and high divergence among species (FST = 0.77), especially between E. edulis and E. precatoria (FST = 0.86). These results showed that, despite the few structural differences among the chloroplast genomes of these Euterpe palms, a differentiation between E. edulis and the other Euterpe species can be identified by point mutations. This study not only brings new knowledge about the evolution of Euterpe chloroplast genomes, but also these new resources open the way for future phylogenomic inferences and comparative analyses within Arecaceae.
Project Titli – product development for women at the bottom of the pyramid
Authors: Chatterjee Debabrata , Kaur Jasleen 
Published: March 2022

Learning outcomes
The learning outcomes are as follows: Understand the concept and characteristics of Bottom of Pyramid (BoP) markets; understand the concept and characteristics of frugal innovations; understand the Design Thinking approach to product design and how it might be useful to develop frugal innovations for BoP markets.

Case overview/Synopsis
The case details the journey of a group of students at a premier engineering college in India. The group aimed to develop and implement a social innovation that addressed a serious and important health issue – menstrual hygiene practices among urban slum dwellers in India. The case begins with how a chance visit to an NGO inspired a pair of students to take up this issue, how the project unfolded at their college, the challenges faced in their journey and, finally, an outcome that was only a partial success. It raises important questions of challenges that are specific to bottom of pyramid markets in emerging economies. The case can provide a context for discussions on approaching frugal innovations from a Design Thinking perspective.

Complexity academic level
This case can be used in social innovation courses/modules at an undergraduate or graduate level in social innovation and social entrepreneurship courses. The case is best positioned towards the beginning of the course as an overview of the process of Social Innovation, and to discuss the relevance of concepts of BoP markets and frugal innovation.

Supplementary materials
Teaching Notes are available for educators only.

Subject code
CSS 7: Management Science.
Perceptions of empowerment and motivation as outcomes of a continuing vocational education and training (CVET) programme for adults
Authors: Mara Liviu-Catalin , Cascón-Pereira Rosalía , Brunet Icart Ignasi 
Published: March 2022

PurposeThe focus of this study is to explore the perceptions of motivation for further training and empowerment in future jobs of participants in different training activities under a public programme implemented in Catalonia (Spain), which delivers continuing vocational education and training (CVET) courses for unemployed and for active workers alike.Design/methodology/approachThe authors used a mixed methodology approach to study the motivation and empowerment perceived in the sample of participants. From an online survey of 281 participants in a CVET programme from the network of public centres that implement the programme in Catalonia, the authors analysed quantitatively the responses and then applied an inductive analysis for the responses related to motivation and empowerment perceived by the participants.FindingsResults show that the participation in this CVET programme has influenced positively the perception of motivation of the majority of participants to enrol in further education or training (80.43%), while at the other end of the spectrum, 18.86% of the participants reported low or no motivation to participate in further education or training. Regarding the empowerment towards their future workplace, 59.43% of participants perceived a high empowerment, while 37.37% reported feeling low-empowered or disempowered.Originality/valueThis is one of the few studies that takes interest in studying a CVET public programme and its potential impact in generating perceptions of motivation for further education or training and empowerment in the participants. Moreover, its implementation was possible due to the collaboration of the public administration, which disseminated the survey to their students.
To stream or not to stream? Exploring factors influencing impulsive consumption through gastronomy livestreaming
Authors: Yu Cheng , Cheah Jun-Hwa , Liu Yide 
Published: March 2022

Purpose
In China, the practice of livestreaming while shopping has evolved from a form of entertainment into a new business strategy. In recent years, the gastronomy industry has also adopted livestreaming as a means of online promotion. Based on the stimulus–organism–response theoretical model, this study aims to investigate the effects of gastronomy livestreaming on viewers’ impulsive consumption by considering gamification, perceived professionalism and telepresence as causative factors.

Design/methodology/approach
This study conducted a survey of gastronomy livestreaming viewers that received 1,093 responses. The effects of gamification, perceived professionalism and telepresence were then analyzed using partial least squares-path modeling and necessary condition analysis.

Findings
This study finds that gamification, perceived professionalism and telepresence are sufficient conditions for explaining impulsive consumption. Innovativeness mediates the relationships between these factors and impulsive consumption. Furthermore, gamification and innovativeness represent necessary conditions for impulsive consumption.

Practical implications
The findings of this study contribute to an enhanced understanding of livestreaming in the gastronomy industry. Based on these findings, managers in the gastronomy industry can use more interactive gamification activities and enhance telepresence to increase viewers’ impulsive consumption during livestreaming sessions.

Originality/value
This study identifies the modalities through which gastronomy livestreaming can stimulate impulsive consumption. This is an early study to investigate the effect of experiences of gamification, perceived professionalism and telepresence on viewers’ impulsive consumption in the context of gastronomy livestreaming. In addition, this early study investigates the effect of gastronomy livestreaming innovativeness on impulsive consumption.
New trends in digital marketing emergence during pandemic times
Authors: Alsukaini Ali Khalaf Mohammed , Sumra Kalsoom , Khan Ruqia , Awan Tahir Mumtaz 
Published: March 2022

Purpose
This study relies on an integrated model to study the role of instant mobile messaging apps in the pandemic. The COVID-19 pandemic is reshaping different forms of businesses; one of them is digital marketing. Many aspects of digital marketing augmented in response to the consequences of the virus. A comparative study between Pakistan and Iraq is conducted to investigate the resistors of innovation with the mediation of intention toward actual system usage. It examines the behavioral intentions and actual behavior of individuals in response to the resistance toward innovation.

Design/methodology/approach
A total of 800 responses were collected through a convenient sampling method from individuals residing in Pakistan and Iraq in the first wave of COVID-19. The data was analyzed through covariance-based structural equation modeling; SPSS and Smart PLS 3.0 were used as efficient data analysis tools in the study.

Findings
The results inferred that individuals are faced with resistance to innovation when they adopt innovative technology. It was inferred that technology adoption is not poised through image both in Pakistan and Iraq. Intention toward actual behavior was determined to be a potential mediator, which enhances the stature of the integrated model.

Originality/value
The significance of this study considering practical and theoretical implications is incorporated for marketer’s policymakers and consumers, along with recommendations for future research.
From market driving to market shaping: impact of a language shift
Authors: Pels Jaqueline , Mele Cristina , Spano Maria 
Published: March 2022

Purpose
Prior research acknowledges the lack of theorizing of markets within marketing. This paper aims to capture the emerging themes linked to different conceptualizations of the market and provide a language system for theorizing the market.

Design/methodology/approach
The paper conducts a bibliometric analysis of 177 articles and 22 keywords and adopts Mele et al.’s (2015) market conceptualization to interpret the results.

Findings
The bibliometric analysis detects five thematic clusters: market driving, market exchange, market shaping, market practices and macro-marketing. Based on their degrees of relevance and development, these are arranged into two groups. The analysis of these two language systems shows a shift toward a more comprehensive conversation on how the market is conceptualized. The authors synthesize this shift under the expression “from market driving to market shaping.”

Research limitations/implications
The comparison of the two language systems allows the argument that market is a complex concept and that to understand it necessitates a rich set of terms.

Practical implications
The different ways of conceptualizing the market mean that managers face a choice when analyzing their market situations (what language system to use). This choice will be consequential for their subsequent actions.

Originality/value
This paper contributes to the identification of two rival language systems and narratives: market driving and market shaping.
Global research on community-based enterprise: a bibliometric portrait
Authors: Pradhan Suchisweta , Samanta Sasmita 
Published: March 2022

Purpose
The purpose of this paper is to provide a comprehensive review of scholarly literature on community-based enterprise (CBE) through a bibliometric analysis and to comprehend the qualitative dimensions of research in this specific field.

Design/methodology/approach
The study is based on scholarly papers indexed in Scopus from 1990 to 2021. The bibliometric analysis focuses on journals, documents, writers, organizations and countries. VOSviewer is used for network visualization mapping of citation, co-citation, bibliographic coupling and co-occurrence of keywords.

Findings
The analysis of the bibliometric aspects of CBE literature reveals an upward trend in publication of CBE documents, with a significant increase of research productivity in the past few years. This behaviour shows that CBE is becoming increasingly popular among academics and practitioners. The document “Toward a theory of community based enterprise” by Ana Maria Peredo is the most cited document. USA has so far published the maximum number of documents in this field.

Practical implications
This study provides an overview of the current state of research in the subject as well as the primary themes explored in this burgeoning discipline, with the potential to help the researchers identify new topics and gaps that need to be investigated further.

Originality/value
This work contributes to the literature by conducting a bibliometric analysis that has not yet been explored. It gives an overview of the field’s organization as well as specifics on the major issues explored in this discipline.
Face-to-face with the new normal: libraries' readiness and perspectives toward the changing service environment
Authors: Abrigo Christine , Torres Efren 
Published: March 2022

PurposeThis paper seeks to explore how library management in academic and school institutions has been adapting to the changes brought about by the global COVID-19 pandemic. It discusses how their priorities have shifted in reinventing their strategic plans and innovative library responses; and their readiness to meet new expectations to support their communities, as necessitated by the changes.Design/methodology/approachEmploying the change readiness concept as a framework, it encompasses inquiry into the library management's response to the changes in the service and work environments at the onset of change, while in the process of adapting to the current changes, and readiness to the perceived changes in the next normal future service environment. Quantitative design was employed for this study, and self-selection strategy as a non-probability sampling technique was used in collecting data from respondents. Target respondents were head librarians. An online questionnaire was used as instrument for data gathering, which was tested valid and reliable.FindingsFindings reveal that respondents have achieved quite a lot in preparing to adapt to change and generally agree that they are ready to deal with the perceived, future-wise changes in all aspects.Research limitations/implicationsThe study was open to all types of libraries as subjects; however, one-person libraries were excluded. Being self-selected, the respondent group for this study is not a true representative of the target population. Data analysis was strictly based on the gathered results.Practical implicationsThe study offers insights on the value of integrating measures pertinent to the emergence of new roles and needs in the changing service environment.Originality/valueThe results of the study are seen to have a potential contribution to the library organization's growth as they oversee their operations, prospectively.