bluuebunny/crossref_metadata_2025_split huggingface dataset:
Existence of three solutions for the fractional p-LAPLACIAN elliptic Dirichlet problems
Authors: Heidarkhani Shapour , Gharehgazloue Fariba , Barilla David , Caristi Giuseppe 
Published: November 2022

Abstract
An optimization problem is formed of maximizing or minimizing a real function. Optimization problems appear in the mathematical modeling of real-world systems and cover a very broad range of applications aris- ing in economics, finance, physics, engineering and computer science. Global optimization has its focus on finding the maximum or minimum over all input values. Global optimization has its focus on finding the maximum or minimum over all input values. In our work, we shall look for local minima for the Euler functional corresponding to the prob- lem. In recent years, a great attention has been focused on the study of fractional and nonlocal operators of elliptic type, both for the pure mathematical research and for concrete real-world applications. In this article, we consider the fractional p-Laplacian elliptic Dirichlet prob- lem that possesses one control parameter and a Lipschitz nonlinearity order of p − 1. The multiplicity of the weak solutions is proved by means of the variational method and critical point theory. We investigate the existence of at least three solutions to the fractional p-Laplacian.
Existence results for a discrete fractional boundary value problem driven by p-Laplacian operator
Authors: Heidarkhani Shapour , Moradi Shahin , Barilla David , Caristi Giuseppe 
Published: November 2022

Abstract
Global optimization concentrates on finding the maximum or mini- mum over all input values. Optimization problems are ubiquitous in the mathematical modeling of real-world systems and cover a very broad range of applications arising in all branches of economics, finance, chemistry, materials science, astronomy, physics, structural and molec- ular biology, engineering, computer science, and medicine. In this paper, we shall discuss the existence of at least one weak solution and infinitely many weak solutions for a discrete fractional bound- ary value problems driven by p-Laplacian operator. In particular, in our work, we shall look for local minima for the Euler functional cor- responding to discrete fractional boundary value problems involving p-Laplacian. On the other hand, equations involving the discrete p- Laplacian operator, subjected to classical or less classical boundary conditions, have been widely studied by many authors using various tech- niques. Our technical approach is based on variational methods. Some recent results are extended and improved. Moreover, some examples are presented to demonstrate the application of our main results.
Assessment of Multiple Model Algorithms to Predict Earthworm Geographic Distribution Range and Biodiversity in Germany: implications for soil-monitoring and species-conservation needs
Authors: Salako Gabriel , Russell David John , Stucke Andres , Eberhardt Einar 
Published: November 2022

Abstract
Identifying the potential distribution of soil-biodiversity with its density and richness relationships, including constituent species, is a pre-requisite for the assessment, conservation and protection of soil biodiversity and the soil functions it drives. Although the role of earthworms in improving soil quality has long been established, to quantitatively and spatially assess how this soil-animal group’s distribution changes along environmental gradients and geographic space and the identification of the drivers of such change has not been fully investigated. This comprehensive study aimed at modelling and mapping earthworm spatial distribution and diversity patterns to determine their conservation needs and provide baseline reference data for Germany. The study compared multiple modelling algorithms to map earthworm community parameters and 12 species-specific distribution probabilities, calculate their geographic range sizes and determine responses to environmental predictor variables. Three general patterns of spatial distribution ranges were identified by the model predictions (large-range, mid-range, and restricted-range species) with the corresponding environmental contributions to the predictions. Modelled species responses to environmental predictors confirm observed environmental drivers of earthworm distribution in Germany. The range classes based both on distributional level and geographic space provide the necessary information for identifying conservation and decision-making priorities, especially for restricted-distribution species as well as those with clearly defined habitat preferences.
Acetylation of Smad1 promotes tumorigenesis and chemoresistance via competitive attenuation of p300-mediated p53 acetylation in glioblastoma
Authors: Gong Lingli , Xu Daxing , Pu Zhening , Zhang Bo , Yin Ying , Ji Li , Wang Jingjing , Hu Yaling , Meng Jiao , Zhang Rui , Fang Xiangming , Jiao Jiantong , Zou Jian 
Published: November 2022

Abstract
Aberrant post-translational modification (PTM) is a key molecular event inciting p53 tumor suppressor function loss or transformation. Here, we report that p53 acetylation, independent of the overall level of p53 expression, predicts improved survival in glioblastoma (GBM). It revealed that acetylation at the C-terminal domain (CTD) reversed tumorigenic gain of function (GOF) of p53 mutants in GBM. An important finding was that acetylation not only strengthened the tumor suppression of wild-type p53, but also reinforced the chemosensitivity of GBM cells with missense mutant p53, as evidenced by attenuated DNA damage repair. Mechanical exploration revealed that the acetylation of p53 in GBM was negatively regulated by Smad1. Smad1 served as an oncoprotein in GBM by promoting tumorigenesis and chemoresistance in a BMP or TGF-β independent manner. It formed a ternary complex with p53 through the MH1 domain and with p300 through the MH2 domain, inhibiting p300-mediated p53 acetylation via competitively binding of the C-terminus of p300. Acetylated Smad1 was identified by p300 at the lysine site 373 (K373). Smad1 acetylation was indispensable due to its transcriptional activity and onco-functions. Collectively, this study highlights that acetylation is critical for p53 and Smad1 functions, and that Smad1 acts as an oncoprotein partially by impairing p53 acetylation in GBM.
CRISPR/Cas9-mediated knockout of IL1RAPL1 in stem cells highlights a role in neural cell migration during cortical development
Authors: Holden Katie , Rath Phalguni , Drydale Edward , Bancroft James , Handunnetthi Lahiru 
Published: November 2022

Abstract

Genetic risk factors contribute to cortical developmental abnormalities observed in a range of neurodevelopmental disorders including autism and schizophrenia. Emerging evidence suggests that IL1RAPL1is a common risk gene across many neurodevelopmental disorders. We combined CRISPR gene editing with induced pluripotent stem cell derived neuronal and microglia model systems to study the function effects of a disease relevant deletion in IL1RAPL1. We investigated several key aspects of human cortical development ranging from radial glia extension, neuronal progenitor migration, excitatory synapse formation and synaptic pruning by microglia. We found that the IL1RAPL1 deletion restricted neural cell migration and excitatory synapse formation in our model system. These findings provide new insight into how rare genetic mutations in IL1RAPL1 can exert deleterious effects on the developing human cortex.
Ultrasound stimulation of piezoelectric nanocomposite hydrogels boosts cartilage regeneration
Authors: Ricotti Leonardo , Cafarelli Andrea , Manferdini Cristina , Trucco Diego , Vannozzi Lorenzo , Gabusi Elena , Fontana Francesco , Dolzani Paolo , Saleh Yasmin , Lenzi Enrico , Columbaro Marta , Piazzi Manuela , Bertacchini Jessika , Aliperta Andrea , Cain Markys , gemmi mauro , Parlanti Paola , Jost Carsten , Fedutik Yirij , Nessim Gilbert , Telkhozhayeva Madina , Teblum Eti , Dumont Erik , Delbaldo Chiara , Codispoti Giorgia , Martini Lucia , Tschon Matilde , Fini Milena , Lisignoli Gina 
Published: November 2022

Abstract
Osteoarthritis implies a progressive degeneration of the whole joint. Cartilage is particularly affected, with inflammation playing a pivotal role1. In recent years, cartilage regeneration has been pursued through several bioengineering strategies and using different stem cell types2-6. Adipose -derived mesenchymal stromal cells (ASCs) constitute an intriguing and minimally invasive option. However, the use of ASCs for cartilage regeneration is hampered by a relatively inefficient expression of key chondrogenic markers7. Thus, new strategies to boost both in situ targeting and chondrogenesis of ASCs are highly desirable. Here we show that ASCs embedded in a nanocomposite hydrogel including piezoelectric nanomaterials and graphene oxide nanoflakes, and stimulated with ultrasound waves with precisely controlled parameters (1 MHz and 250 mW/cm2, for 5 min once every two days for a period of 10 days) dramatically boost cell chondrogenic commitment. Furthermore, this stimulation regimen also has a considerable anti-inflammatory effect. The proposed nanocomposite hydrogel also shows excellent biocompatibility in vivo. Our results show for the first time the chondrogenic potential of the combined piezoelectric nanoparticle-ultrasound stimulus; the proposed paradigm has the potential to trigger cartilage regeneration in osteoarthritis, focal cartilage defects and other pathological conditions involving cartilage lesions and degeneration. Future efforts should expand preclinical data, and target clinical applications of this therapeutic strategy.
Description of the demicyclic life cycle of Puccinia sherardiana in Sphaeralcea angustifolia in Mexico.
Authors: Sánchez-Pale Jesús Ricardo , Leal-León Ivan , QUEZADA-SALINAS ANDRES , Moreno-Velazquez Magnolia 
Published: November 2022

Abstract
During 2017 and 2018, plants with dark brown lesions containing hypophilic telia surrounded by chlorotic halos were collected on leaves and stems of Sphaeralcea angustifolia located in Axapusco, Estado de México. Based on the morphological characteristics of pycnia, aecia and telia observed in light microscopy and scanning electron microscopy, the fungus Puccinia sherardiana was identified. No uredinal status was present during the observation period. Verification of identity was performed by phylogenetic analysis with a ribosomal DNA 28S gene sequence. Additionally, pathogenicity tests were performed on leaves of S. angustifolia by inoculation of teliospores. The inoculated plants developed symptoms 15 days after inoculation, beginning with the presence of errupent aecia in the epidermis of the host and later telia were formed, completing Koch's Postulates. Puccinia sherardiana was previously described as a rust with a microcyclic life cycle on species of the genera Alcea, Malvastrum, Sidalcea and Sphaeralcea, belonging to the Malvaceae family, however, this study showed that this phytopathogenic fungus has a demicyclic life cycle.
Mechanical and Functional Property Investigation of 2-Layered Pervious Concrete
Authors: Spoorthy B.M , Chandrappa Anush K. , Sahoo Umesh C. 
Published: November 2022

Background:
Pervious concrete pavements are considered one of the well accepted stormwater management strategies as per environmental protection agencies. The interconnected pore structure in the pervious concrete allows the stormwater to percolate and thus reduce the runoff.

Methods:
The laboratory and field investigations on the pervious concrete pavements have shown that the abrasion and clogging are major distresses that reduce the functional performance of these sustainable pavements. The open graded structure of the pervious concrete undergoes ravelling due to vehicular movement and they may also allow the suspended particles in stormwater runoff to get clogged inside the pore structure. In this study, 2-layered pervious concrete was designed to balance the trade-offs between strength and durability. The porosity and density of 2-layered pervious concrete were found to be in the domain of conventional pervious concrete.

Result:
The failure pattern in compression indicated that the failure of the specimen was concentrated on the bottom layer compared to the top layer. Further, the compressive strength was found to be in the range of 11.90 to 20.0 MPa. The pore distribution at the interface of the top and bottom aggregate was found to follow 3-parameter Weibull distribution, where the scale parameter increased as the aggregate size increased. The abrasion resistance was found to be lower compared to conventional pervious concrete.

Conclusion:
Overall, it was found that 2-layered pervious concrete can be one of the alternatives to implement pervious concrete pavements addressing the demerits of conventional pervious concrete.
Measurement of the Hygric Resistance of Concrete Blocks with Perfect Contact Interface: Influence of the Contact Area – Complementary Comments
Authors: Delgado J.M.P.Q. , Guimarães A.S. , Ribeiro I. , Freitas V.P. 
Published: November 2022

Introduction:
In March 2021, this journal published the paper “Measurement of the Hygric Resistance of Concrete Blocks with Perfect Contact Interface: Influence of the Contact Area”. This commentary aims to provide readers with a set of complementary comments that seek to clarify a few issues that can be raised.

Methods:
The analysis was done based on the original paper “Measurement of the Hygric Resistance of Concrete Blocks with Perfect Contact Interface: Influence of the Contact Area”. The purpose was to complete and comment on the work developed in the original paper, and to clarify some points that might be less understood.

Results and Discussion:
Some interesting questions are presented, and the analysis results intend to clarify them, namely: (1) the magnitude of the quantified post-interface flows; (2) the distinguishability of the moisture absorption in the monolithic and perfect contact samples; (3) the robustness of the knee-point identification algorithm; (4) the dependability of the capillary absorption measurements; (5) the consistency of the capillary absorption processing; (6) the number and “quality” of samples that should be used.

Conclusion:
The conclusions to highlight are the following: the hygric resistance results would be different as they consider different methodologies for the knee point detection and a different number of data points after the knee (different ones) to calculate the slope; the monolithic samples reached the highest moisture masses, Mw, and the Mw values became lower with the interface occurrence; for the knee-point identification, it was only considered valid the use of the third of the three algorithms described in Section 2.3; the taping of the samples was carefully done, and absorption tests using epoxy resin is considered a better solution; the C calculation was made for all monolithic samples, but only the 3 more representative experimental results for each contact area were represented (as mentioned) and the Aw of the 10x10 cm2 cross-section should be 0.1013 kg/m2s0.5, which does not influence the conclusions/findings.
Experimental Investigation of the Forklift Truck Impact on CFS Pallet Racks
Authors: Gusella Federico , Orlando Maurizio 
Published: November 2022

Background:
Storage pallet racks are commonly formed by Cold-Formed-Steel (CFS) members and are used worldwide to store goods on pallets. The main racking system is denoted as “selective pallet racking”. This racking system is one pallet deep and is separated by aisles, allowing for each pallet, stored on horizontal beams, to be always accessible. Steel racking systems are frequently subjected to accidental impact forces from operating forklift trucks. If international racking design codes provide an arbitrary value of impact force to design members, several impacts can produce damages, which can lead to system failure, highlighting the fundamental role played by monitoring.

Methods:
Results of an experimental campaign on a full-size selective rack are presented. The investigated rack is subjected to the impact of a forklift truck and hammer test at different points. The propagation of the acceleration among adjacent bracing frames is investigated with the magnitude of the recorded strains in structural members.

Results:
Results highlight that an accelerometer every two spans can establish whether the monitored racking system is accidentally hit. Compressed diagonal and tensile diagonal work in parallel. Only one diagonal brace, every two spans, can be monitored through a strain gauge to establish the forklift truck impact point and estimate the stress distribution on adjacent members.

Conclusion:
The study suggests an optimization in the number, type and position of accelerometers and strain gauges in monitoring racking systems to identify forklift truck impact and its effects.
Implementation of Mechanistic-Empirical Pavement Design Guide against Indonesian Conditions using Arizona Calibration
Authors: Subagio Bambang Sugeng , Prayoga Aldo Budi , Fadilah Siti Raudhatul 
Published: November 2022

Background:
As a transportation infrastructure that connects one area to another, roads have an essential role in economic and social growth, together with establishing a location that may improve the quality of life in the surrounding community. For this reason, it is necessary to perform road maintenance when the structural or functional capacity of the road is inadequate, one of which is by overlaying the road.

Objective:
The main objective of this research is to determine the thickness of the flexible pavement overlay and subsequently examine the damage model produced by the 2015 MEPDG method with Arizona calibration. This study also proposes recommendations for implementing the 2015 MEPDG procedures in Indonesian settings.

Methods:
The thickness of the road overlay can be designed through a mechanistic-empirical approach, which is commonly referred to as the Mechanistic-Empirical Pavement Design Guide (MEPDG). The back calculation on the BAKFAA program was utilized to examine the existing situation. At the same time, a stress-strain analysis was performed using the KENPAVE software to calculate the response of the pavement structure.

Results:
The 2015 MEPDG with Arizona calibration by controlling fatigue cracking has resulted in an overlay thickness of 180 mm. In addition, the damage model was obtained for each type of road failure and can be beneficial in estimating the future IRI value.

Conclusion:
The damage model generated from the 2015 MEPDG procedure is specific to the types of road damage, which can eventually be utilized to predict the future IRI value. It also encompasses local and global calibration variables, such as adjustment factors for its implementation in road pavement conditions in Indonesia. The MEPDG application can be simplified by shortening the mechanistic analysis process, along with reducing traffic variations and the level of detail in the daily climate analysis.
Bioinformatics Analysis of the Rothia dentocariosa Proteome and Assessment of the Proinflammatory Potential of Biofilm and Planktonic Cells
Authors: Alshatti Fatmah , Almutairi Rahaf , Alkandari Shareefa , Haneef Asma , Bhardwaj Radhika , Karched Maribasappa 
Published: November 2022

Background:
Rothia dentocariosa is an opportunistic pathogen found in the oral cavity and is found to be involved in many oral infections as it has the ability to attach to the tooth and mucosal surfaces, produce substantial amounts of acids and integrate into dental plaque biofilms.

Aim:
To analyze the proteome of R. dentocariosa by using bioinformatics tools and to investigate the proinflammatory potential of R. dentocariosa.

Materials and Methods:
Protein sequences of R. dentocariosa were downloaded from NCBI and various in silico analyses were performed using bioinformatics tools. R. dentocariosa CCUG 35437 was grown on blood agar in 5%CO2 in air at 37 C for 2 days. Biofilms were cultured for 2 days and quantified by crystal violet staining. Human whole blood was stimulated with biofilms, biofilm-supernatants, planktonic cells, and whole cells. Proteome Profiler and ELISA-based quantification of cytokines were performed for the samples.

Results:
In silico analysis of the whole genome and proteome of R. dentocariosa revealed a number of proteins predicted to be potentially secreted but also possess virulence properties. R. dentocariosa was able to form only moderate biofilms. The ability of R. dentocariosa to induce different cytokines varied depending on the stimulant being used. Biofilms and planktonic cultures induced specific cytokines that were not induced by whole cells or biofilm supernatants. While IL-8 was induced at near-similar levels from biofilm and planktonic cells, IL-10 was induced at significantly higher levels (P<0.05) only by the planktonic cultures. The biofilm-supernatant and the whole cell stimulants induced lower levels of cytokines than biofilm and planktonic cultures.

Conclusion:
Identification of potential virulence factors predicted to be secreted extracellularly may suggest a key role for R. dentocariosa in oral and non-oral infections. Different stimulants from R. dentocariosa showed varying potential to induce cytokines from human whole blood. This may suggest differences in the composition/concentration of the bacterial components in the stimulants, with varying abilities to induce cytokine production, maybe the reason for the observed differences.
Knowledge of General Practitioner’s toward Spondyloarthritis (SpA): A Qualitative Study
Authors: Pratama Mirza Zaka , Wahono Cesarius , Mukti Nabila , Rahman Perdana 
Published: November 2022

Background: Low back pain (LBP) is one of major health problems experienced by 80-85% of patients in their lifetime. Spondyloarthritis (SpA) has become one of the leading causes of chronic LBP but is often undiagnosed. General practitioners (GP) have an essential role in the early diagnosis of SpA.
Aim: To explore the knowledge of GP about SpA from clinical diagnosis and early management and elaborate on each GP’s clinical perspective and practice performance.
Methods: A qualitative study that involved 12 GP. All was consented to participate in an in-depth interview with the experts by online meeting with 15-20 minutes duration. Analysis was focused on the definition, classification, clinical manifestation, diagnosis, and early management of SpA in primary care.
Results: All GP were interviewed (12 GP, 9 men, and 3 women; mean age 29.42) with average years in clinical practice was 3.8 years. All GP could describe the definition of SpA. Only a few subjects are aware of the subtype of SpA. Most of the GP could mention chronic back pain as the main SpA symptom, some GP mentioned extra-articular manifestation, but incomplete. All GP understood the current treatment in clinical practice.
Conclusion: GP is aware of SpA, but not all could completely mention the type and clinical entities. In addition, a limited resource for investigation makes a diagnosis of SpA difficult. Current early treatment has been acceptable in clinical practice. A comprehensive understanding of diagnosis and effective early treatment may reduce delayed diagnosis and improve patients' quality of life.