bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein Kinase D1 Overexpression Potentiates Epidermal Growth Factor Signaling Pathway in Mcf-7 Cells
Authors: LEGAY Christine , DOUBLIER Sophie , BABAJKO Sylvie , RICORT Jean-Marc 
Published: July 2022

Abstract
I. Background: Protein kinase D1, PKD1, is a serine-threonine kinase implicated in cell proliferation, migration, invasion, and/or apoptosis and its activation by several growth factors sets this enzyme as a key regulator of tumorigenesis and tumor progression. Despite many studies, its role in the regulation of intracellular signaling pathways remains widely disparate and needs to be clarified.II. Methods and Results: By using human breast cancer cells MCF-7, overexpressing or not PKD1, we demonstrated that PKD1 expression level modulated the tumor growth-promoting epidermal growth factor (EGF) signaling pathway. We also showed that EGF acutely stimulated PKD1 phosphorylation with similar time courses both in control and PKD1-overexpressing cells. However, PKD1 overexpression specifically and markedly increased EGF-induced phosphorylation of Akt (onto T308 and S473 residues) and extracellular-regulated protein kinase (ERK1/2). Finally, pharmacological inhibition of PKD1 activity or lowering its expression level using specific siRNAs drastically reduced EGF-stimulated Akt and ERK phosphorylation in PKD1overexpressing cells, but not in control cells.III. Conclusions: Overall, these results identified the level of PKD1 expression as a key determinant in the regulation of the EGF signaling pathway highlighting its crucial role in a tumorigenic setting.
Predicting the next word using the Markov Chain Model according to profiling personality
Authors: ELBAGHAZAOUI Bahaa Eddine , AMNAI Mohamed , FAKHRI Youssef 
Published: July 2022

Abstract
Understanding human data has been the focus of philosophers and scientists. Social media platforms encourage people to be creative and share their personal information. By analyzing data, we will be able to identify people's personalities and information that is also important to specific profils. The aim of this paper is to propose an approach that predicts the next word during writing a sentence based on the user's personality. To achieve this goal, our approach is illustrated by two points: (1) An approximate extraction of the Big Five model for a specific user from his tweets. (2) Predicting the next word while a user is writing a new tweet depends on his personality using the Markov Chain Model. On the basis of these two notions, our approach makes writing posts easier by predicting and suggesting next words based on the user's personality. Experience represents the ease of predicting the next word during the writing of a new post related to individual potential.
Multigenerational analysis of the ecotoxicological effects of paracetamol on the expression of genes HR96 and GST in Daphnia magna
Authors: Olivares René , Pedreros Pablo , Valenzuela Francisca , Aguayo Claudio , Urrutia Roberto 
Published: July 2022

Abstract
Various studies have investigated the effects of paracetamol on D. magna, with abundant information on acute and chronic toxicity, but little on multigenerational molecular-level assays. Therefore, this study is focused on a multigenerational investigation of the effects of paracetamol exposure at a constant concentration of 1 mg L− 1 on the expression of the cellular detoxification genes HR96 and GST in D. magna over three generations. The effective concentration of this compound was also determined by studying daphnid survival. The acute exposure results showed mean effective concentrations of EC50 = 11.74 mg L− 1 (11.07–12.48) and EC1 = 1.63 mg L− 1 (1.40–2.62). The multigenerational analysis showed a significant inhibition of both genes in both generation F0 (HR96 = 32.6%, GST = 33.2%) and generation F1 (HR96 = 27.1%, GST = 20.2%). Meanwhile, in F2 a significant induction was observed for GST and HR96, with values of 10.8% and 13.5%, respectively. The response of D. magna over the generations presented a clear induction trend, indicating that this organism is capable of adapting to the contaminant after multiple generations of exposure. Thus, this study shows that paracetamol is more toxic during the first generations and that after 30 days, the drug would not affect the cellular detoxification system.
Enhanced Toxic Dye Degradation Using Single Crystal Zn-doped Fe3O4 Octahedrons
Authors: Nguyen Xuan Sang 
Published: July 2022

Abstract
Single crystalline octahedral Zn doped Fe3O4 was successfully prepared through a simple hydrothermal method without the assistance of any surfactant or template. Porosity and crystalline properties characterized by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and high resolution transmission electron microscopy (HRTEM). Results showed as-synthesized Zn doped Fe3O4 are uniform octahedrons with high crystalline. In addition, the possible mechanism for the formation of octahedral products via the time-dependent experiments and the role of hydrazine hydrate in the synthesis process was also discussed. Their photo-Fenton activity in photodegradation of Rhodamine B under visible light was sequentially investigated, indicating the high photocatalytic capacity of as-obtained octahedrons.
Investigation of a suspected nosocomial infection outbreak in a neonatology department, China
Authors: TANG Wenrui , Cheng Peiyu , TANG Manjuan 
Published: July 2022

Abstract
Objective
To identify the risk factors for the neonatal infections; to determine the infectious sources, and to initiate prevention and treatment processes.
Materials and Methods
The case group consisted of 5 infected neonates. The control group consisted of 10 premature infants without infection, but with similar gestational age, body weight, and with > 48 hours stay in hospital. t and chi-square tests were used for comparison between the two groups and P < 0.05 was considered significant. Environmental samples in the clinic were collected from the two groups from 9th October to 10th November, 2020. Cultures were initiated to detect sources of infectious agents.
Results
There were no significant differences in gender, gestational age, birth weight, delivery mode, maternal age, invasive ventilator, umbilical vein between the two groups (P > 0.05). For the case group, antibiotic application (type and duration of treatment), length of hospital stay, Peripherally Inserted Central Catheter (PICC) catheterization time and gastric tube in-dwelling time were significantly higher than those in control group (P < 0.05). Pseudomonas aeruginosa was identified from sputum and skin cultures from infected neonates, and from multiple faucet samples.
Conclusion
The case groups had significantly more infection-related features than those in the control group. The Pseudomonas aeruginosas outbreaks were associated with exogenous water sources but the infectious agent had different drug resistant patterns. The data, therefore, allowed us to exclude the infection as NI outbreak but to conclude exogenous NIs. Our report also indicates the effectiveness of having pre-determined protocols and expert personnel to handle the suspected outbreak efficiently, and to execute successful prevention and intervention processes.
Knee Joint Proprioceptive Acuity and Kinematic Control in Relation to Motor Competency in 13 to 14-Year-Old Adolescents
Authors: Liu Yan-Ci , Esser Patrick , Weedon Benjamin David , Springett Daniella , Joshi Shawn , Tsou Meng-Hsuan , Wang Ray-Yau , Dawes Helen 
Published: July 2022

Abstract
Motor competence (MC) is a key component reflecting one’s ability to execute motor tasks and is an important predictor of physical fitness. For adolescents experiencing rapid growth changes, understanding the factors affecting MC is pertinent to the development of more sophisticated sporting skills. Previous studies considered the influence of poor proprioceptive ability on MC, however, the relationship between lower limb proprioception and MC is unknown, and the relationship between kinematic control and MC is not well understood. Therefore, the aim of this study was to determine to what extent lower limb proprioception (according to knee joint position sense), and kinematic control relate to MC in adolescents. This study was a cross-sectional design. Young people (n = 427, 196 girls and 231 boys) aged 13 to 14 years were recruited. A movement reproduction task was used to assess joint position sense and quality of performance, while the Movement Assessment Battery for Children (mABC-2) was used to assess MC. In addition, this study categorized 80 adolescents who met the criteria according to their mABC-2 scores as the Developmental Coordination Disorder (DCD) group, while 231 adolescents were categorized as Typically Developed (TD), to further compare motor competency. Results showed that kinematic data, specifically normalized jerk, showed a significant correlation with MC. No correlation between knee joint position sense and MC. No group differences between DCD and TD were found. From these results, it may be concluded that rather than proprioception, there is a greater association between MC and kinematic control and sensorimotor integration.Clinical trial registration number : NCT03150784
MiR-217 regulated autophagy through OPG/RANKL/RANK in giant cell tumor
Authors: Jiang Boyong , Meng Chenyang , Wang Lianjuan , Liu Wanlin , Zhao Zhenqun , Bai Rui , Zhao Yan 
Published: July 2022

Abstract
Aims
Increasing evidences have suggested that microRNAs (miRNAs) play crucial roles in cancer development and progression. Our previous study showed that the level of miR-217 was remarkably lower in GCT cells and tissues, and the re-expression of miR-217 induced an inhibitory effect on occurrence and development of GCT in vitro. However, the mechanisms underlying the proliferation inhibition effect of miR-217 in GCT cells still remain unknown. Thus, this article is aimed to explore the mechanisms underlying the proliferation inhibition effect of miR-217 in GCT cells.
Methods
The GCT cells proliferative potential was measured by use of the MTT assay and BrdU straining. The changes in migration and invasion of GCT cells was determined by transwell assay. Finally, western blot and RT-PCR assays were employed to evaluate the expression of OPG/RANKL/RANK signaling pathway related-proteins.
Result
In the present study, the excessive upregulation of miR-217 markedly suppressed GCT cell proliferation and tumorigenesis in vitro and in vivo. Meanwhile, the overexpression of miR-217 could inhibit OPG/RANKL/RANK signal pathway in vitro and in vivo. Furthermore, the ALP activity was also significantly decreased in GCT cells by miR-217 treatment. Importantly, miR-217 could inhibit autophagy-related protein expression and autophagosomes/autolysosomes formation in GCT cells and tissues.
Conclusion
These results suggest that the upregulation of miR-217 inhibit the occurrence and development of GCT through inhibiting autophagy. This study also offers an effective therapeutic target to improve the survival rates of patients with CGT in the future.
Arteannuin-B and its Spiro-isoxazoline Derivative Inhibits LPS Induced Pro-Inflammatory Responses in RAW 264.7 Macrophages and BALB/c Mice via down-regulation of NF-κβ/P38 MAPK Mediated Stress Signalling
Authors: Sawhney Gifty , Rasool Javeed Ur , Saroch Diksha , Ozturk Mumin , Parihar Suraj , Brombacher Frank , Ahmad Bilal , Bhagat Asha , Ali Asif , Ahmed Zabeer 
Published: July 2022

Abstract
Amongst the most significant scientific findings in the last two decades has been the role of inflammatory processes in protecting the host against the causative agents of injury. In this work, we examined the anti-inflammatory activities of Arteannuin-B (1) and its novel spirocyclic-2-isoxazoline derivative (2). Various anti-inflammatory assays were performed in lipopolysaccharide (LPS) induced RAW 264.7 macrophages using enzyme-linked immunosorbent assay, flow cytometric analysis, and western blotting. As a result, treatment with (2) decreased LPS-stimulated inducible nitric oxide synthase protein expression (iNOS), as well as production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) in LPS induced RAW 264.7 cells. Additionally, the effects of (1) and (2) in an acute inflammatory condition were investigated in-vivo by using models like carrageenan-induced paw edema assay, acetic acid-induced writhing and tail immersion model in experimental mice, suggesting that (2) is a more potent anti-inflammatory candidate as compared to (1) with very low cell toxicity. In addition, biocomputational studies and histopathological analysis were also performed to validate the efficiency of (2) as compared to (1).
The effects of projected climate change on crop water availability in the U.S. Caribbean using CMIP6 models
Authors: Moraes Flávia D. S. , Ramseyer Craig , Gamble Douglas W. 
Published: July 2022

Abstract
Recent studies have suggested that anthropogenic climate change mainly affects the Small Island Developing States (SIDS), and farming systems in the Caribbean are vulnerable to climate change, especially changes in temperature and precipitation, due to their relatively high dependence on rainfall. However, there is a lack of research investigating the local impacts of climate change on agriculture in the U.S. Caribbean. This work evaluated how temperature and precipitation projections could affect water crop needs in Puerto Rico (Greater Antilles) and St. Croix (Lesser Antilles). We used Daymet climate data to create a baseline climatology (1981–2010), and the Coupled Model Intercomparison Project Phase 6 (CMIP6) to create two future climatologies (2041–2070 and 2071–2100). The Thornthwaite water budget model was used to estimate water deficit, and the CROPRISK model to determine crop suitability for sweet pepper, banana, and plantain. Results indicated that water stress after 2041 is expected to be greater for most of the region from June–August, except for western Puerto Rico, where water deficit will be greater from January–March. For sweet pepper, banana, and plantain agroclimatic suitability, the most water-stressed season is projected to be January–July, mainly for southern Puerto Rico and St. Croix. November will be the only month during which all regions and all crops were projected to be highly suitable through the end of the 21 st Century. These findings suggested that Puerto Rico and St. Croix crop water stress may be more sensitive to future changes in temperature than changes in precipitation. Understanding the location and periods most sensitive to water stress and crop suitability should help local governments to plan for mitigating future food insecurity in the U.S. Caribbean.
Establishment of a new practical telesurgical platform using the hinotori™ Surgical Robot System: A preclinical study
Authors: Nakauchi Masaya , Suda Koichi , Nakamura Kenichi , Tanaka Tsuyoshi , Shibasaki Susumu , Inaba Kazuki , Harada Tatsuhiko , Ohashi Masanao , Ohigashi Masayuki , Kitatsuji Hiroaki , Akimoto Shingo , Kikuchi Kenji , Uyama Ichiro 
Published: July 2022

Abstract
Aim
The recent development of new surgical robots and network telecommunication technology has opened new avenues for robotic telesurgery. Although a few gastroenterological surgeries have been performed in the telesurgery setting, more technically demanding procedures including gastrectomy with D2 lymphadenectomy and intracorporeal anastomosis have never been reported. We examined the feasibility of telesurgical robotic gastrectomy using the hinotori™ Surgical Robot System in a preclinical setting.
Methods
First, the suturing time in the dry model was measured in the virtual telesurgery setting to determine the latency time threshold. Second, a surgeon cockpit and a patient unit were installed at Okazaki Medical Center and Fujita Health University, respectively (approximately 30 km apart), and connected using a 10-Gbps leased optic-fiber network. After evaluating the feasibility in the dry gastrectomy model, robotic distal gastrectomies with D2 lymphadenectomy and intracorporeal B-I anastomosis were performed in two porcine models.
Results
The virtual telesurgery study identified a latency time threshold of 125 ms. In the actual telesurgery setting, the latency time was 27 ms, including a 2-ms telecommunication network delay and a 25-ms local information process delay. After verifying the feasibility of the operative procedures using a gastrectomy model, two telesurgical gastrectomies were successfully completed without any unexpected events. No fluctuation was observed across the actual telesurgeries.
Conclusion
Short-distance telesurgical robotic surgery for technically more demanding procedure may be safely conducted using the hinotori Surgical Robot System connected by high-speed optic-fiber communication.
Random fractal-enabled physical unclonable functions with dynamic AI authentication
Authors: Sun Ningfei , Chen Ziyu , Wang Yanke , Wang Shu , Xie Yong , Liu Qian 
Published: July 2022

Abstract
Physical unclonable functon(PUF) is an indispensable fundament in anti-counterfeiting due to its inherent uniqueness. However, self-limitation of conventional graphical/spectral PUFs in materials often causes it difficult to simultaneously possess high code flexibility and excellent environmental stability in practice. Here we propose a universal, fractal-guided film annealing strategy to realize the random Au network-based PUFs that can be designed on-demand in complexity, enabling the tags’intrinsic uniqueness and stability. Based on the roughening-enabled plasmonic network platform, an additional chemical encoding with enhanced Raman response can be demonstrated for multiple-level security. Moreover, a dynamic deep learning-based authentication system with an expandable database is built for identifying and tracking the PUFs, realizing an efficient and reliable authentication with 100% validation accuracy. The configurable and multidimensional tags in mass production can be served as the competitive PUF carriers for high-level anti-counterfeiting and data encryption.
Gene Expression Profiling Analysis to Investigate the Role of CIMT after Stroke in Neurological Behaviors
Authors: Sun Xiaoyu , Xu Haichun , Zheng Tianyi , Liu Liang , Qu Huiling 
Published: July 2022

Abstract
Objectives
Constraint induced movement therapy (CIMT) promoting neurogenesis, synaptic plasticity and axon regeneration after stroke in animal models, but the candidate genes and pathways associated with these changes are unknown. The present study was conducted to explore the effects of CIMT on motor function and cognitive function in stroke-induced rats.
Methods
The study included three groups: sham-operated rats (n = 6, SHAM), rats subjected to cerebral ischemia (n = 8, ISC), rats with cerebral ischemia plus CIMT(n = 8, CIMT). A cerebral infarction rat model was established by transient middle cerebral artery occlusion (tMCAO). CIMT began on the 8th day after the cerebral ischemia surgery by fitting a plaster cast around the unimpaired upper limbs of rats for 3 weeks. The neurological scoring test was performed to evaluate the neurological function of rats. Stable Platform Test and Coat Hanger Test were performed to evaluate the motor function. The Morris water maze(MWM)test was conducted to assess the cognitive function of rats. RNA sequencing and bioinformatics analysis were employed to study the underlying mechanisms.
Results
We observed that CIMT significantly ameliorate the memory dysfunction as well as neurological deficits in cerebral ischemic rats caused by tMCAO. We identified a total of 105 DEGs, including 71 upregulated genes and 34 downregulated genes. GO analysis revealed that these DEGs were associated with the major categories: at the MF level, the DEGs were mainly enriched in N-acetylgalactosamine 4-O-sulfotransferase activity, lyase activity, glutathione transferase activity and oxidoreductase activity, acting on paired donors. KEGG analysis showed that the DEGs were mainly enriched in 4 categories: environmental information processing, human disease, metabolism, organismal system.
Conclusions
We identified the following 16 genes with the most significant differential expression: En1, Cpne7, Siglec5, Chchd4, Fcrl2, Trhr, Myom3, Piezo2, Ankrd34b, Slc6a3, Rftn1, Chst9, Gstm6, Cd34 and Tgm2. These findings prompt exploration of the potential mechanisms and key genes involved in the effect of CIMT.
Research on dry shrinkage behavior of bamboo based on the fibers content and arrangement of vascular bundles in the cross section
Authors: Zhang Ying , Xu Haocheng , Li Jing , Zhong Tuhua , Wang Hankun 
Published: July 2022

Abstract
Bamboo is easily affected by moisture, resulting in dimensional instability or even cracking. In this paper, based on manual measurement and vascular bundle detection model, the changes in appearance, shape, areas of cross section and fiber sheath, fiber volume fraction, and arrangement of vascular bundles of the bamboo ring during dry shrinkage process were studied. The results showed that the dry shrinkage of fibers was greater than that of parenchyma cells. The dry shrinkage stress decreased from the outside to the inside along the bamboo wall with the fiber volume fraction, gradually causing an increase in radius, a decrease in radian and arc length, and the generation of cracks in the bamboo ring. The radial dry shrinkage rate was 8.14%, slightly larger than the tangential dry shrinkage rate of 6.02%, and both were much larger than the longitudinal dry shrinkage rate of 0.48%. This work further revealed the dry shrinkage mechanism of bamboo, the partition and marking method used in this research might be meaningful for the quantitative study of bamboo structural changes at the micro-scale.
Low dose of Esmolol attenuates sepsis-induced immunosuppression via modulating T-lymphocyte apoptosis and differentiation
Authors: Ma Ying , Cheng Zhenshun , Zheng Yong , Wang Wei , He Shaojun , Zhou Xiaolian , Yang Jiong , Wei Chaojie 
Published: July 2022

Abstract
Background
Persistent immunosuppression characterized by immune cells apoptosis and T- lymphocyte subsets imbalance, occurs in septic patients with poor outcomes. Recent studies reported that Esmolol improved survival in septic shock via modulating immune responses. We hypothesized Esmolol alleviate sepsis-induced immunosuppression by regulating immune cell apoptosis and differentiation and sought to investigate the potential mechanisms and optimal dose.
Methods
Four hours after cecal ligation and puncture (CLP), Wistar rats were randomized into CLP, CLP + E-5 (Esmolol:5mg.kg− 1.h− 1) and CLP + E-18 (Esmolol:18mg.kg− 1.h− 1) group. Another 8 rats were under sham operation. 18 hours after CLP, hemodynamics and organ histological injuries were evaluated, peripheral blood mononuclear cells apoptosis and circulating T-lymphocyte subsets counts were determined by flow cytometry, protein expressions of p-Akt, Bcl-2, cleaved Caspase-3 and p-Erk1/2 were determined by western blot and immunohistochemistry. mRNA and protein expressions of β1-adrenoreceptors were evaluated by quantitative real-time PCR and immunohistochemistry.
Results
CLP induced tachycardia, hypotension, hyperlactatemia and multiple organ injuries. Infusion of Esmolol at 5mg.kg− 1.h− 1 didn't reduce heart rate, but improved mean arterial pressure, lactatemia and multiple organ injuries, decreased circulating T-lymphocyte apoptosis and the ratio of T help 2 cells and T help 1 cells, unregulated p-Akt and Bcl-2 expression and down-regulated cleaved Caspase-3 and p-Erk1/2 expression.
Conclusions
Esmolol without heart rate reduction attenuated sepsis-induced immunosuppression by preventing T-lymphocytes from apoptosis through regulating Akt/Bcl-2/Caspase-3 pathway and reducing naive CD4+ T cells differentiation to T help 2 cell through inhibiting Erk1/2 activation. Esmolol may be a potential immune regulator in septic shock.