bluuebunny/crossref_metadata_2025_split huggingface dataset:
A prospective, randomised comparison of KingVision, Airtraq videolaryngoscopes and TotalTrack video intubating laryngeal mask for face-to-face intubation in morbidly obese patients
Authors: Nowak-Tim Justyna , Gaszynski Tomasz , Ratajczyk Pawel 
Published: April 2022

Abstract
Backgroud: Traditional endotracheal intubation demands unlimited access to the patient and possibility to stand behind his head. However, in case of difficult conditions in emergency settings we can use an alternative method. Face-to-face intubation can be performed in patients in semi-erect position, prone position and in the situation of difficult access to the head. Method: After obtaining an approval from the Local Ethics Committee Nr RNN/62/20/KE and written informed consent from the patients, we performed in 30 patients who were scheduled for planned operations, randomly divided into 3 subgroups of using KingVision or Airtraq videolaryngoscopes or TotalTrack video intubating laryngeal mask. Results: The intubation time was comparable between devices: 9.25 ±2.05 s vs 8 ±2.14 s (p=0.2322) in KingVision and Airtraq videolaryngscopes respectively and significantly longer for TotalTrack VILM: 23.5 ±3.89 sec. All three devices allowed for successful intubation in all cases. Both videolaryngoscopes appeared to deliver an optimal view of the entrance to larynx and TotalTrack worse but acceptable. All devices enable the operator to intubate with face-to-face method without any complications. Conclusions: KingVision and Airtraq videolaryngoscopes and TotalTrack VILM during face-to-face intubation allowed for front-access endotracheal intubation and ensured an good view of larynx entrance. We assume that in case of difficult access to the patient’s head or untypical position, the usage of examined video laryngoscopes, can be considered.
Can sacralization cause morphological changes in lumbar and abdominal muscles?
Authors: ÇANKAL Fatih , UÇAR İlyas , ÇINAROĞLU Selim , KARARTI Caner 
Published: April 2022

Abstract
Purpose
Although sacralization is one of the most common congenital anomalies of the spine, its effect on surrounding muscles is still not well known. This study was conducted to determine the size of the lumbar and abdominal muscles of the individuals with sacralization and to compare with the control group.
Methods
Eighty-five participants with sacralization phenomena and fifty-six asymptomatic participants were included in this study. Sacralization was classified according to the Castellvi classification. The cross-sectional area of the multifidus lumborum, erector spinae and rectus abdominis muscles, and the section thicknesses of external abdominal oblique, internal abdominal oblique and transversus abdominis muscles were measured bilaterally on axial computed tomography images at the L1-2 and L4-5 levels.
Results
ANCOVA revealed that there is no any significant group*side interaction effect regarding muscles sizes in terms of two groups in L1-L2 and L4-L5. In addition, ANCOVA revealed a both side effect [(p = .020; η2p = .038)] and group*side interaction effect [(p = .010; η2p = .049)] regarding length of the L5 transverse process (p˃.05).
Conclusions
Regardless of low back pain, muscle sizes are not associated with the sacralization phenomenon.
Nectin-4 promotes osteosarcoma progression and metastasis through activating PI3K/AKT/NF-κB signaling by down-regulation of miR-520c-3p
Authors: Liu Yongheng , Li Guanghao ,   Jinyan_feng@126.com Feng, Li Lili , Zhang Yan , Zhang Yanting , Huang Xiaoyu , Xu Yao , Zhang Jin , Wei Xianfu , Shen Yuxiang , Zhang Zhongmin , Han Xiuxin , Zhang Chao , Zhang Jin , Zhang Chao , Ma Yuling , Wang Guowen 
Published: April 2022

Abstract

Purpose
Nectin-4 was specifically up-regulated in various tumors, exerted crucial effects on tumor occurrence and development. Nevertheless, the role and molecular mechanism of Nectin-4 in osteosarcoma (OS) were rarely studied.
Methods
The expression of Nectin-4 and its relationship with clinical characteristics of OS were investigated using OS clinical tissues, tissue microarrays, TCGA and GEO databases. Moreover, the effect of Nectin-4 on cell proliferation, migration, and invasion abilities were detected by in vitro functional assays, respectively. The RT-qPCR, Western blotting and luciferase reporter assays were performed to explore molecular mechanism which Nectin-4 mediated the expression of miR-520c-3p, thereby modulating PI3K/AKT/NF-κB signaling. In vivo mice models were used to validate the functional roles of Nectin-4 and miR-520c-3p.
Results
Nectin-4 displayed a higher expression in OS tissues compared with normal tissues. We also found that Nectin-4 overexpression was positively associated with tumor stage and metastasis in OS patients. Besides, the miR-520c-3p was negatively correlated with Nectin-4, and its low expression is closely related with tumor metastasis in OS patients. Functionally, Nectin-4 enhanced cells proliferation and cell mobility in vitro. Moreover, mice models with subcutaneous xenograft and lung metastasis confirmed that knockdown of Nectin-4 could suppress the tumorigenesis and tumor metastasis in vivo. Mechanistically, Nectin-4 promoted epithelial-mesenchymal transition (EMT) by activating PI3K/AKT/NF-κB signaling through down-regulation of miR-520c-3p.
Conclusions
This study firstly clarified that Nectin-4 promoted OS progression and metastasis through activating PI3K/AKT/NF-κB signaling mediated by negative regulation of miR-520c-3p, which would open a novel avenue for identifying potential therapeutic target to improve patient outcomes.
A New Model in Medicine Education Smart Model Education Set
Authors: Taş Fatih , Bolatlı Güneş 
Published: April 2022

Abstract
Purpose
Education set consisting of three-dimensional smart interactive models with audio, visual, and light features and an application program that allows learning branches of science such as anatomy and histology at the same time can bring innovation to medicine and health education. Our study aims to show contributions of this education set, which we have patented, to student academic success and medical education.
Methods
The students participating in study consisted of three groups. Students were divided into Group 1 (classical education), Group 2 (smart model education set and theoretical expression), and Group 3 (smart model education set). Pre-test, post-test, and state anxiety scale applications were made to all groups before and after education. Trait anxiety scale was administered before education. Education set used in study includes hardware and software parts.
Results
Post-education state anxiety scale in Group 1 mean was significantly higher than Group 2 and 3 mean; Group 2 mean was significantly higher than Group 3 mean. There was no significant difference between groups regarding trait anxiety levels. It was observed that there was a significant difference between the pre-test and post-test in all three groups. The increase in post-test achievement level of Group 2 and 3 was significantly higher than Group 1.
Conclusions
Smart model education set integrates basic and clinical information. Mobile application will ensure continuity of theoretical and practical education at desired place and time. Invention will bring a new breath to basic medical education by preventing inequalities in medical and health sciences education.
Multistrain Probiotic mixture VSL#3 ameliorates inflammation of Gut-Liver axis in bacterial endotoxin Lipopolysaccharide exposed mice
Authors: Babu Gyan , Mohanty Banalata 
Published: April 2022

Abstract
The gastrointestinal (GI) tract mucosa serves as the barrier limiting dissemination of pathogenic microbes, microbial components and other food associated toxins to reach general circulation. When this barrier disrupts in diseases such as GI tract inflammation, pathogenic bacteria, microbial components and other toxins translocate from the gut lumen in to systemic circulation. Liver is in direct vascular connection with gut through portal circulation and is highly susceptible to inflammation on exposure to these toxins. The present study demonstrated that bacterial cell wall endotoxin lipopolysaccharide/LPS induced inflammation of both gut and liver of mice. Intraperitoneal exposure of mice to LPS (1mg/kg bw/day) caused histopathological alterations of both Jejunum-ileum and colon of GI tract. Distinct histopathological alterations of liver were observed as well. Increased plasma pro-inflammatory cytokines IL-6 and TNF-α and decreased of antioxidant defence enzymes Super oxide dismutase/SOD and catalase in both gut and liver of LPS-exposed mice indicated them as the biomarkers of inflammation. Increased serum level of transaminases (ALT & AST) further indicated hepatic inflammation. Supplementation of commercial probiotic VSL#3, a combination probiotic (eight different species of bacteria belonging to three different genera, Lactobacilli, Bifidobacteria and Streptococcus) for a month (4 weeks: 0.6g/kg bw/day) attenuated the inflammation decreasing the plasma pro-inflammatory cytokines, serum ALT and AST, and reducing the oxidative stress as reflected in the improved histological architecture of both gut and liver. Thus, the modulation of intestinal microbiota is critical to maintaining homeostasis of the gut-liver axis and an emerging therapeutic strategy in treatment of gut dysbiosis and hepatic pathology.
Response to PD-1 Blockers Alone or Combined with Anlotinib in Treatment of Anaplastic and Poorly Differentiated Thyroid Carcinoma : A Real-world Study to Identify Best-benefited Patients
Authors: Ma Yifei , Wang Youlong , Zhu Pengfei , Zeng Jiling , Hu Ying-Ying , Wu Hao , Shi Huazheng , Fan Wei , Wang Xinjia 
Published: April 2022

Abstract
BackgroundAnaplastic and poorly differentiated thyroid carcinoma (ATC/PDTC) have poor survival outcomes. Mono-therapy with either immuno-therapy or tyrosine kinase blockers (TKI) is sparsely reported and seems not to significantly affect prognosis or survival. We sought to testify whether combined therapies have a better outcome in real-world settings. Pathological plus PET/CT volumetric biomarkers were analyzed to find crosstalk associated with response.MethodsPatients receiving PD-1 blockers alone or combined with anlotinib were recruited to undergo sequential PET/CT scanning. Propensity score-matched analysis was carried out for comparison of treatment outcomes. Structure equation modeling was carried out to investigate the crosstalk among sensitive biomarkers. A nomogram was developed and validated using the patients in the same centers.ResultsA total of 69 patients received either combined or mono-therapy with a clinical benefit rate of 88.41% (61 / 69) and tolerable adverse events, with a mean survival time of 14.64 months. The mean survival time of the combined therapy group was 15.67 months. The mean improvement of total lesion glycolysis (TLG) for the combined group was 120.07 (29.42% improved from baseline). Angiogenic marker VEGFR, FGFR, and VEGF mediate the PD-1 blockade impact on TLG improvement.ConclusionAnti-angiogenic agents anlotinib could potentiate PD-1 blockade by diminishing angiogenesis or its downstream effects. The combined treatment increased survival and responses could be better evaluated by volumetric PET/CT parameters. Pathological biomarker expression levels were associated with TLG improvement in combined treatment.
Organelle resolved proteomics reveals new chordoma cell surface markers required for proliferation and association with outcome
Authors: Khan Shahbaz , Zuccato Jeffrey , Ignatchenko Vladimir , Singh Olivia , Govindarajan Meinusha , Waas Matthew , Gao Andrew , Zadeh Gelareh , Kislinger Thomas 
Published: April 2022

Abstract
BackgroundChordomas are rare, clinically aggressive tumors with a median survival of 6-7 years and a high rate of disease progression despite maximal surgery and radiotherapy. Given the limited options available to prevent and treat progression and relatively poor prognosis, there remains an urgent need for the development of novel therapies to improve clinical outcomes. Cell-surface proteins are attractive therapeutic targets due to their accessible subcellular localization.MethodsHere, we used a proteomics approach to identify novel chordoma-specific cell-surface protein markers. Four established chordoma cell lines were analyzed by quantitative proteomics using a comprehensive differential ultracentrifugation organellar fractionation approach. A subtractive proteomics strategy was applied to select proteins that are plasma membrane enriched. Using commercially available antibodies, the expression profiles of these cell-surface proteins were validated across chordoma cell lines, patient surgical tissue samples, and normal tissue lysates via Western blotting. The candidates were further validated by immunohistochemical analysis in a 25-patient tissue cohort. Finally, the essentiality of these candidates for in vitro chordoma growth was evaluated.ResultsMass spectrometry-based proteomics identified 120 high-confidence cell-surface proteins in four established chordoma cell line models. Systematic data integration prioritized two chordoma-specific cell surface proteins for further interrogation. Immunohistochemistry in a richly annotated cohort of chordoma tumor tissues revealed that PLA2R1 and SLC6A12 are broadly expressed in chordoma patient samples. Higher expression of PLA2R1 correlated with poor prognosis whereas SLC6A12 expression was significantly enriched in skull-base chordomas compared to those arising in the spine. Using a siRNA-mediated knockdown of PLA2R1, we demonstrated significant inhibition of cell growth and colony-forming ability, suggesting these proteins play an essential role in chordoma biology.ConclusionWe have comprehensively elucidated the proteome of four established chordoma cell lines. Subtractive proteomics and integrative data mining revealed novel cell-surface proteins required for chordoma cell survival and associated with clinical parameters in a small chordoma tissue sample cohort.
Microcrustaceans inhabiting tropical freshwater contaminated and non contaminated with arsenic: identification of regional suitable bioindicators.
Authors: Mendoza-Chávez Yadira J. , Uc-Castillo Luis , Gutiérrez-Aguirre Martha A. , Cervantes-Martínez Adrián , Martínez-Villegas Nadia 
Published: April 2022

Abstract
We investigated microcustraceans inhabiting arsenic contaminated and non contaminated freshwater in order to identify those absent in arsenic contaminated water as suitable bioindicators of arsenic contamination in tropical freshwater in mining and metallurgical areas in northern Mexico. For doing so, we collected zooplankton, water and sediment samples, at five sampling points in two sampling campaigns, to determine water temperature, pH, electrical conductivity (EC), dissolved oxygen (DO), alkalinity (Alk), salinity (Sal), and total arsenic concentration in water and sediments samples. We additionally determine arsenic mobility from sediments and its speciation. We also identified microcrustacean species and determined abundance, richness and Shannon Index. Results showed that the maximum arsenic concentration in freshwater was 53.23 mg/L, while the minimum arsenic concentration was < 0.01 mg/L. Arsenic concentration in sediments was between 10.37 mg/kg and 2472.84 mg/kg, with high arsenic mobility (up to 100%) where the arsenic leached was As(V). Nine species of microcrustaceans were found. Simocephalus punctatus, Alona glabra, Eucyclops leptacanthus, Macrocyclops albidus and Pleuroxus (Picripleuroxus) quasidenticulatus, inhabiting arsenic-free water, Latonopsis australis, Eucyclops chihuahuensis, Acanthocyclops americanus, Pleuroxus (Picripleuroxus) quasidenticulatus, Macrocyclops albidus and Paracyclops chiltoni, inhabiting moderately and highly contaminated water. Simocephalus punctatus (Orlova-Bienkowskaja, 1998) was absent in contaminated water bodies in study area, and therefore we proposed this microcustracean could serve as a bioindicator of water quality in waterbodies contaminated with arsenic in study area and northern Mexico, where arsenic contamination is common ground.
Systematic Review and Meta-analysis of Environmental Toxic Metals Contaminants and Risk of Ischemic Stroke
Authors: Dev Priya , Gupta Priya , Mahapatra Archisman , Cyriac Mareena , Kumar Amit , Singh Varun Kumar , Mishra Vijaya Nath , Pathak Abhishek 
Published: April 2022

Abstract
BackgroundStroke is the second largest cause of mortality (WHO 2014) and long-lasting disability worldwide. Many risk factors are associated with stroke: age, gender, chronic illnesses, cardiovascular disease, lifestyle, and smoking. With global industrialisation, the roles of environmental contaminants and their association with stroke are still unclear and have attracted much more attention.MethodologyWe conducted a systematic review on environmental toxic metals contaminants and the risk of ischemic stroke. A comprehensive literature search was carried out till 30th June 2021 from databases such as PubMed, Science Direct, Embase and Scopus. The quality of all the articles which met our inclusion criteria was assessed using Newcastle-Ottawa scaling, and four eligible studies were included for our systematic review.ResultThe serum and urine cadmium concentrations were positively associated with the risk of ischemic stroke. There is an inverse association of serum and urine concentration of Mercury (Hg), serum concentration of gold and cerium with ischemic stroke, and the serum and urine concentration of Lead (Pb) had no association with ischemic stroke risk.ConclusionThe study showed strong associations between heavy metals and ischemic strokes, but more studies are required to prove the associations.
Prediction of microvascular obstruction by coronary artery angiography score after acute ST-segment elevation myocardial infarction：a single-center retrospective observational study
Authors: Li Ziwen , Yin Hongbin , Wang Dehua , Zhang Yayun , Feng Yongli , Zhou Yi , Zhou Ying 
Published: April 2022

Abstract
Background: Some coronary artery angiography scores are associated with no-reflow phenomenon after percutaneous coronary intervention (PCI) in patients with acute ST-segment elevation myocardial infarction (STEMI). However, quality evidence about the association between coronary artery angiography score and microvascular injury is still needed. Our study aimed to validate the ability of coronary artery angiography score in predicting microvascular obstruction (MVO) detected by cardiac magnetic resonance (CMR) imaging.Methods: From October 2020 to October 2021, 141 consecutive patients with acute STEMI who underwent primary PCI and CMR were reviewed retrospectively. CMR were performed between 3 to 7 days after PCI. Patients were divided into MVO group and non-MVO group based on the CMR results. Two coronary artery angiography scores (SYNTAX score and Gensini score) were used to assess the severity of coronary artery atherosclerotic burden.Results: A total of 122 patients were included (mean age 60.6 ± 12.8 years). MVO occurred in 51 (41.8%) patients. Patients with MVO had higher SYNTAX scores (p < 0.001) and Gensini scores (p < 0.001) than those without MVO. Gensini score (r =0.580, p < 0.001) showed relative stronger correlation with infarction size than SYNTAX score (r =0.521, p < 0.001). The areas under the receiver operator characteristic curves of SYNTAX score and Gensini score for predicting MVO patients were 0.726 and 0.805. In multivariable regression analysis, peak troponin I (odd ratio [OR] = 1.196, p = 0.001), left ventricular ejection fraction (LVEF) (OR = 0.915, p = 0.021) and Gensini score (OR = 17.567, p = 0.016) were identified as independent predictors of MVO.Conclusions: In patients with acute STEMI undergoing primary PCI treatment, Gensini score showed relative stronger correlation with infarction size than SYNTAX score and Gensini score may be an independent predictor of MVO.
Human albumin administration is associated with increased in-hospital mortality in heart failure with hypoproteinaemia patients: analysis of MIMIC-III Database
Authors: Liu Xuebo , Yao Tongqing , Qian Jun , Chen Fei , Lin Hao , Lai Yan 
Published: April 2022

Abstract
Background: Heart failure (HF) is a growing health problem worldwide, especially in the senior. To overcome diuretic resistance in inpatients with HF and hypoproteinaemia, some doctors administer albumin combined with diuretics, though clinical studies on the use of albumin in patients with HF were rare. We aimed to clarify the effect on mortality of administering human albumin during management of HF inpatient especially with hypoproteinaemia.Methods: We extracted 6094 HF patients from Medical Information Mart for Intensive Care III (MIMIC-III) database. Receiver operating characteristic (ROC) curve was used to elucidate the relationship between serum albumin content and in-hospital mortality. Multivariate logistic regression models，propensity score matching (PSM) and lowess smoother curve were employed to analyze the relationship between albumin administration and clinical outcomes. The clinical outcomes contained in-hospital mortality, output at 24-hour, ICU length of stay (LOS) and hospital LOS. In addition, Kaplan-Meier (KM) curve was used to assess 90-day survival in albumin administration and non-albumin administration subgroups before and after PSM.Results: Patients with heart failure, serum albumin concentration is negatively correlated with risk of in-hospital death. Based on ROC curve, the optimal cut-off value for predicting the mortality were 2.9g/dl (area under curve (AUC)= 0.643). Albumin administration was significantly associated with increased mortality during hospitalization in the HF plus serum albumin≤2.9g/dl group (p<0.001), but no significant difference in serum albumin>2.9g/dl group (p= 0.25). After PSM in serum albumin≤2.9g/dl group, hospital LOS and ICU LOS were prolonged (p<0.001, p<0.001), output at 24-hour had no association with albumin infusion (p= 0.229), 90-day mortality was no significant difference between albumin administration and non-albumin administration patients with albumin≤2.9g/dl after PSM (p= 0.12). Conclusion: Albumin administration was associated with increased risk of in-hospital mortality, as well as longer hospital LOS and ICU LOS among HF patients with hypoproteinaemia.
Finite element modelling on the effect of the height-to-diameter ratio of bilayer iron powder compact
Authors: Yusoff Syamimi Mohd , Tahir Suraya Mohd , Ariff Azmah Hanim Mohammed , Supeni Eris Elliandy , Anuar Mohd Shamsul 
Published: April 2022

Abstract
In this study, finite element modelling (FEM) had been implemented to capture the changes in interfacial local relative density (RD) distribution between compressed layers of a green bilayer iron powder compact system. Validation work had been taken as an initial work before undergoing several modelling tests on different green bilayer iron compacts with specified height-to-diameter (H/D) ratios. Our proposed experimental framework with a new mapping technique was used to gather the details of RD distribution on a real green single (Sample A1) and a bilayer iron sample (Sample A2). We produced the desired experimental local RD distribution under a magnification of 100X via an optical microscope and visualization through Tecplot software. An effective prediction on local RD distribution on single green iron powder compacts allowed for further assumption in layered samples. To proceed, four kinds of H/D ratios of 1.0 (Sample A2), 1.3 (Sample B), 1.6 (Sample C) and 1.9 (Sample D) were assigned for layers with the same thickness. Via modelling, observation on the highest interfacial local RD distribution had been successfully captured for H/D ratios of 1.0, followed by 1.3 and 1.6. It had been revealed that the green bilayer iron powder compacts efficiently delivered its densification as well as minimization on its density gradient compared to the green single iron powder compact.