bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-cell transcriptome analysis reveals heterogeneity and a dynamic regenerative response of quiescent radial glia in adult zebrafish brain
Authors: Kutsia Manana , Takeuchi Yuki , Ranawat Nishtha , Masai Ichiro 
Published: July 2022

AbstractIn zebrafish telencephalon, radial glial cells (RGs) show a remarkable ability to regenerate damaged neural tissue by re-initiating cell proliferation to produce neural precursors to rebuild the lost neural circuit. However, it is not fully understood how RGs respond to brain damage to initiate this regenerative response. Here we applied single-cell transcriptomics to RGs in adult zebrafish telencephalon and identified five RG subtypes, which are classified into four quiescent RGs (qRGs) and one proliferating RG (pRG). The four qRGs differentially express distinct subsets of qRG markers, suggesting heterogeneity of qRG in zebrafish adult brain. Interestingly, one qRG subtype shows high expression of ribosomal proteins, and its fraction increases in response to brain damage. Consistently, the mTOR pathway is activated in RGs near the injury site. It was reported that inflammatory responses of brain-resident immune cells, microglia, are required for inducing regenerative responses of RGs in zebrafish. Genetical elimination of microglia not only suppressed the damage-induced regenerative response of RGs, but also decreased the fraction of the ribosomal expression-enriched qRGs. Our pseudo-time analysis suggests that putative dormant RGs produce ribosomal expression-enriched qRGs through activation of ribosomal genesis, as well as suppression of cholesterol biogenesis, and pRGs through activation of the JAK/STAT pathway. Our findings reveal heterogeneity of qRGs in adult zebrafish brain and their dynamic regenerative response to brain damage.
Ubiquitination of the GluA1 subunit of AMPA receptors is required for synaptic plasticity, memory and cognitive flexibility
Authors: Guntupalli Sumasri , Park Pojeong , Han Dae Hee , Ringuet Mitchell , Blackmore Daniel G. , Jhaveri Dhanisha J. , Koentgen Frank , Widagdo Jocelyn , Kaang Bong-Kiun , Anggono Victor 
Published: July 2022

SUMMARYActivity-dependent changes in the number of AMPA-type glutamate receptors (AMPARs) at the synapse underpin the expression of long-term potentiation (LTP) and long-term depression (LTD), cellular correlates of learning and memory. Post-translational ubiquitination has emerged as a key regulator of the trafficking and surface expression of AMPARs, with ubiquitination of the GluA1 subunit at Lys-868 controlling the post-endocytic sorting of the receptors into the late endosome for degradation, and thereby regulating their stability at synapses. However, the physiological significance of GluA1 ubiquitination remains unknown. In this study, we generated mice with a knock-in mutation in the major GluA1 ubiquitination site (K868R) to investigate the role of GluA1 ubiquitination in synaptic plasticity, learning and memory. Our results reveal that these mice have normal basal synaptic transmission but exhibit enhanced LTP and deficits in LTD. They also display deficits in short-term spatial memory and cognitive flexibility. These findings underscore the critical roles of GluA1 ubiquitination for bidirectional synaptic plasticity and cognition.
Identification and full genome analysis of the first putative virus of sea buckthorn (Hippophae rhamnoidesL.)
Authors: Balke Ina , Zeltina Vilija , Zrelovs Nikita , Kalnciema Ieva , Resevica Gunta , Ludviga Rebeka , Jansons Juris , Moročko-Bičevska Inga , Segliņa Dalija , Zeltins Andris 
Published: July 2022

AbstractThe agricultural importance of sea buckthorn (Hippophae rhamnoidesL.) is rapidly increasing. Several bacterial and fungal pathogens infecting sea buckthorn have been identified and characterized; however, the viral pathogens are not yet known. In this study, we identified, isolated, and sequenced a virus from a wild plantation of sea buckthorn for the first time. Sequence analysis of the obtained viral genome revealed high similarity with sequences of several viruses belonging to the genusMarafivirus, especially olive latent virus 3 (OLV-3). The genome of the new virus is 6,989 nucleotides (nt) in length according to 5′ and 3′ rapid amplification of cDNA ends (RACE) (without polyA-tail), with 5′ and 3′ untranslated regions being 133 and 109 nt long, respectively. The viral genome encoded two open reading frames (ORFs). ORF1 encoded a polyprotein of 1,954 amino acids (aa) with the characteristic marafivirus non-structural protein domains—methyltransferase, Salyut domain, papain-like cysteine protease, helicase, and RNA-dependent RNA polymerase. ORF1 was separated from ORF2 by a six nt and encoded the coat protein (CP). CP had typical signatures of minor (30.96 kDa) and major (21.18 kDa) forms. Both CP forms were cloned and expressed in a bacterial expression system, and only the major CP was able to self-assemble into 30 nm virus-like particles that resembled the native virus, thus demonstrating that minor CP is not essential for virion assembly. We suggest the newly discovered virus to be named as “Sea buckthorn marafivirus”, abbreviated as “SBuMV”.Author summarySea buckthorn is an exceptionally valuable plant that is currently widely cultivated as multipurpose horticultural species for food, pharmacology, cosmetics, and landscape conservation. Diseases and pests directly affect the cultivation of SBT. To date, several pests and diseases, mainly fungal and bacterial, but no viral, sea buckthorn have been reported. Identification of new pathogens would assist in the development of control strategies, and quarantine purposes and ensure sustainable sea buckthorn cultivation. Here, for the first time, we present a virus putatively infecting sea buckthorn. We had characterized its full genome, cloned and expressed minor and major forms of coat protein either individually or co-expressed. We also showed that major coat protein-derived virus-like particles self- assembled directly in the bacterial cells, and the majority of the expressed CPs were soluble. Our study suggests that the minor CP is not essential for the assembly of seemingly structurally intact viral particles, meaning that it can have other functions.
Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cells
Authors: Sugimoto Chie , Fujita Hiroyoshi , Wakao Hiroshi 
Published: July 2022

AbstractThe function of mucosal-associated invariant T (MAIT) cells, a burgeoning member of innate-like T cells abundant in humans and implicated in many diseases, remains obscure. To explore this, mice with a rearranged T cell receptor (TCR) α or β locus, specific for MAIT cells, were generated via induced pluripotent stem cells derived from MAIT cells and designated Vα19 and Vβ8 mice, respectively. Both mice expressed large amounts of MAIT cells. The MAIT cells from these mice were activated by cytokines and an agonist to produce IFN-γ and IL-17. While Vβ8 mice showed resistance in a cancer metastasis model, Vα19 mice did not. Adoptive transfer of MAIT cells from the latter into the control mice, however, recapitulated the resistance. This has implications for understanding the role of MAIT cells in health and disease and in developing treatments for the plethora of diseases in which MAIT cells are implicated.
Root organogenesis induction in Epipremnum aureum stem cuttings with plant biostimulants and synthetic rooting hormone
Authors: Villafuerte D.E. , Angeles E. , Bayog A. , Duka R. , Meñoza N.L. , Sanchez M.A. , De Jesus R. 
Published: July 2022

AbstractPlant organogenesis induction is a vital method to regenerate explants and produce complete organisms. In this study, we analyzed the applicability of three different root biostimulants and a commercially available synthetic rooting hormone (RH) for root organogenesis induction in Epipremnum aureum stem cuttings. The biostimulants used were Aloe vera gel (AV1), and garlic (GR2) and turmeric extracts (TM3), and the synthetic RH (TakeRoot®) used contained an active ingredient, indole butyric acid (0.01%). The E. aureum stem cuttings were placed in hydroponic pots and root development rates were monitored for up to 30 days. Recorded data from five parameters were analyzed: (1) number of rooted cuttings, (2) number of roots per stem cutting, (3) length of the longest and (4) shortest roots of the cuttings, and (5) rooting time. Stem cuttings were quantified using ImageJ software. The results showed that compared to the application of TakeRoot®, treatment with the biostimulant AV1 produced the longest roots, whereas stem cuttings treated with GR2 and TM3 did not produce significant results. Moreover, AV1 induced root organogenesis 16.67% faster than did TakeRoot® but no significant difference (p<0.05) was observed in the case of number of roots promoted per cutting. This study provides scientific evidence for the application of naturally derived RHs in the propagation of stem cuttings. Furthermore, Aloe vera gel, known for plant growth benefits, is the best choice for plant root propagation.
Pathogenicity of novel Monosporascus species in accessions of four melon botanical groups
Authors: Costa Francisco Cleilson Lopes , Paiva Negreiros Andréia Mitsa , Sales Rui , de Sousa Nunes Glauber Henrique 
Published: July 2022

AbstractThe Monosporascus root rot and vine decline (MRRVD) can be caused by the fungal species Monosporascus cannonballus and M. eutypoides. The Monosporascus species recently described in Brazil (M. brasiliensis, M. caatinguensis, M. mossoroensis, M. nordestinus and M. semiaridus) are potentially causal agents of the MRRVD. This work was made to evaluate them comparing to M. cannonballus pathogenicity, and to evaluate melon accessions reaction in four botanical groups of melon. MRRVD was evaluated by the severity of the damages in the roots and by the root dry matter reduction index (RIDM). On average, the studied species caused damage to melon accessions. After all, only M. brasiliensis, M. caatinguensis and M. nordestinus were virulent according to the accessions evaluated (A-16, C-32 syn. Pat 81, ‘Goldex’ and ‘HBJ’), i.e., accession-species interaction occurred, and among them, M. caatinguensis was the most virulent. The accession A-16 (acidulus group) showed higher resistance (<10 % of root dry mass loss) to M. cannonballus, M. caatinguensis and M. nordestinus. The accession C-32 was susceptible to M. caatinguensis and moderately resistant to the others. The accession A-16 was the most promising one and can be used as a donor of resistance alleles or as a rootstock.
Crystal structures of glutamyl-tRNA synthetase from Elizabethkingia anopheles and E. meningosepticum
Authors: Brooks Lauryn , Subramanian Sandhya , Dranow David M. , Mayclin Stephen J. , Myler Peter J. , Asojo Oluwatoyin A. 
Published: July 2022

Elizabethkingia bacteria are globally emerging pathogens that cause opportunistic and nosocomial infections, with up to 40% mortality among the immunocompromised. Elizabethkingia species are in the pipeline of organisms for high-throughput structural analysis at the Seattle Structural Genomics Center for Infectious Disease (SSGCID). These efforts include the structure–function analysis of potential therapeutic targets. Glutamyl-tRNA synthetase (GluRS) is essential for tRNA aminoacylation and is under investigation as a bacterial drug target. The SSGCID produced, crystallized and determined high-resolution structures of GluRS from E. meningosepticum (EmGluRS) and E. anopheles (EaGluRS). EmGluRS was co-crystallized with glutamate, while EaGluRS is an apo structure. EmGluRS shares ∼97% sequence identity with EaGluRS but less than 39% sequence identity with any other structure in the Protein Data Bank. EmGluRS and EaGluRS have the prototypical bacterial GluRS topology. EmGluRS and EaGluRS have similar binding sites and tertiary structures to other bacterial GluRSs that are promising drug targets. These structural similarities can be exploited for drug discovery.
Measuring diversity, equity and inclusion: a holistic approach
Authors: Chaudhry Smita 
Published: July 2022

Purpose
This paper aims to explore how organizations can measure diversity, equity and inclusion, and what are the potential areas where they can take initiatives to meet the diversity, equity and inclusion goals.

Design/methodology/approach
This paper explains the distinction among diversity, equity and inclusion. It elaborates on the separate perspectives that organizations need to adopt to define and measure each one of them. It consequently brings attention to their implications for research, practice and society.

Findings
This paper reveals that the goals of diversity, equity and inclusion, although related, need to be approached independently. The three concepts need to be measured individually and separate initiatives need to be designed and implemented to attain those goals.

Originality/value
This paper highlights the conceptual differences among diversity, equity and inclusion, and their evaluation. It underscores that diversity itself is not sufficient in ensuring organizational performance. Most of the discussion around implementing diversity pays little attention to steps that need to be taken to ensure equity and inclusion of the diverse workforce. This paper seeks to address this gap.
Entropy and institutional theory
Authors: Aksom Herman 
Published: July 2022

PurposeOnce introduced and conceptualized as a factor that causes erosion and decay of social institutions and subsequent deinstitutionalization, the notion of entropy is at odds with predictions of institutional isomorphism and seems to directly contradict the tendency toward ever-increasing institutionalization. The purpose of this paper is to offer a resolution of this theoretical inconsistency by revisiting the meaning of entropy and reconceptualizing institutionalization from an information-theoretic point of view.Design/methodology/approachIt is a theoretical paper that offers an information perspective on institutionalization.FindingsA mistaken understanding of the nature and role of entropy in the institutional theory is caused by conceptualizing it as a force that counteracts institutional tendencies and acts in opposite direction. Once institutionalization and homogeneity are seen as a product of natural tendencies in the organizational field, the role of entropy becomes clear. Entropy manifests itself at the level of information processing and corresponds with increasing uncertainty and the decrease of the value of information. Institutionalization thus can be seen as a special case of an increase in entropy and a decrease of knowledge. Institutionalization is a state of maximum entropy.Originality/valueIt is explained why institutionalization and institutional persistence are what to be expected in the long run and why information entropy contributes to this tendency. Contrary to the tenets of the institutional work perspective, no intentional efforts of individuals and collective actors are needed to maintain institutions. In this respect, the paper contributes to the view of institutional theory as a theory of self-organization.
Parliamentary engagement in fighting corruption: a discourse on the Parliament of Tanzania
Authors: Lukiko Lukiko Vedastus 
Published: July 2022

Purpose
This study aims to examine the participation of the Parliament of Tanzania in the fight against corruption in the country.

Design/methodology/approach
A desk-based research approach based on the review of documents and legal instruments was used.

Findings
The results of this study show that the Parliament of Tanzania has a chequered performance in fighting corruption. While it has passed several anti-corruption-related laws and in some respects succeeded to hold a few government officials accountable for the abuse of public office, there is little evidence to demonstrate its contribution at fighting this conundrum. Factors contributing to this deficiency include irresponsiveness to corruption allegations involving Members of Parliament, parliament’s remote oversight of the anti-corruption agency and shrinking democratic space in the parliament.

Practical implications
Tanzania has relatively high corruption levels. The country’s Development Vision 2025 envisages a nation free of corruption. Hence, efforts are needed from public and private sectors to overcome this conundrum. The parliament holds a special place in that fight. Through its representation, legislative and oversight roles and powers, parliament has a wider opportunity to strengthen anti-corruption in the country. This study shows that the Parliament of Tanzania has not been very effective in that regard. It offers suggestions to strengthen the parliament’s position and engagement to fight corruption.

Originality/value
There is scanty literature on the role of the Parliament of Tanzania in fighting corruption. This study is seminal, as it investigates the Tanzanian anti-corruption arsenal from a crucial organ that is vested with constitutional powers to make laws and oversee the executive and its agencies.
Intersection control with connected and automated vehicles: a review
Authors: Wu Jiaming , Qu Xiaobo 
Published: July 2022

Purpose
This paper aims to review the studies on intersection control with connected and automated vehicles (CAVs).

Design/methodology/approach
The most seminal and recent research in this area is reviewed. This study specifically focuses on two categories: CAV trajectory planning and joint intersection and CAV control.

Findings
It is found that there is a lack of widely recognized benchmarks in this area, which hinders the validation and demonstration of new studies.

Originality/value
In this review, the authors focus on the methodological approaches taken to empower intersection control with CAVs. The authors hope the present review could shed light on the state-of-the-art methods, research gaps and future research directions.
Unintended consequences of knowledge management during the COVID-19 pandemic in 2021: the case of Netflix
Authors: Oh Ingyu , Fei Li , Rowley Chris 
Published: July 2022

Purpose
Unintended consequences of knowledge management (KM) can be harmful if they are calamitous. However, they can occasionally be advantageous during catastrophes. The purpose of this study is to investigate how KM can be accidentally propitious during the COVID-19 pandemic using the case of Netflix.

Design/methodology/approach
Explanatory factor analysis, multilevel and multiple regressions were used with a sample of 45 countries.

Findings
In the authors’ sample, the hypothesized direct relationship between culture (i.e. individualism, power distance and indulgence) and collective pandemic resilience (CPR) was found. In addition, the hypothesized moderating effect of Netflix KM on the relationship between culture and CPR was partially confirmed. The findings suggest that KM during the pandemic can generate an unintended consequence of intensifying the degree of CPR.

Research limitations/implications
Small sample size, data paucity and the constructed variable of CPR might limit the generalizability of this study’s results. Nonetheless, one important research implication is that KM qua unintended consequences can have a significant moderating effect on the relationship between culture and resilience.

Practical implications
This paper highlights how organizations and society can cocreate the value of KM accidentally for the benefit of a larger public during calamities. Also, firms should proactively search for a wider application of their KM beyond their original intention.

Originality/value
This paper initiates a new discussion of positive consequences of unintended KM. Unlike individual-level studies of collective resilience in the past, to the best of the authors’ knowledge, this study generates country-level implications for the first time.
Using product lifecycle management (PLM) to re-think fashion business education: an assessment of pedagogical practices and learning benefits
Authors: Conlon Jo 
Published: July 2022

Purpose
Organisations are investing in systems such as product lifecycle management (PLM) to support product development, collaboration across complex supply chains and to provide a framework for digital transformation. Graduates of apparel programmes would benefit from a knowledge of PLM to help realise the opportunities that PLM offers. The purpose of this paper is to report on an educational research project that used PLM as a context for practice-based learning and as a mechanism to update the learning experience and stimulate the development of future practice.

Design/methodology/approach
This paper reports on the experiences, critical reflections and data from an action research study to establish a learning community through an educational partnership for PLM software within an undergraduate fashion business course. The cohort of the first year of the intervention (n = 28) is the main study population.

Findings
The findings indicate that PLM provided a stimulating learning context supportive of a detailed understanding of current industry practice, critical and innovative thinking and the development of a professional identity.

Research limitations/implications
The opportunity for the development of both industry and educational practice is outlined.

Practical implications
A general introduction to PLM provides important information to support and advance Fashion Industry 4.0. Educational partnerships can reduce barriers to the integration of advanced technologies into the higher education curriculum.

Originality/value
Applications of PLM are under researched in textiles and apparel. The paper contributes to the broadening of the knowledge base of PLM and its potential to achieve strategic transformation of the sector.
Floral and pollination biology of the critically endangered insular orchid Calanthe amamiana: Implications for in situ conservation
Authors: Sugiura Naoto 
Published: July 2022

AbstractCalanthe amamiana is a terrestrial orchid that thrives on the shady forest floors of broad‐leaved forests on Amami Island, central Ryukyus, Japan. Here, I report on the floral and pollination biology of this critically endangered orchid based on a 7‐year study conducted in the species' natural habitats. As the nectarless flowers are probably self‐compatible but are unable to autonomously self‐pollinate, they need to attract pollen vectors by deceit for pollination. To advertise themselves, the flowering inflorescences, which comprise a few to dozens of flowers, use whitish floral colors that are comparatively conspicuous against the shaded background. The exclusive pollinator was the long‐horned bee Eucera okinawae, which nests in soils of the forest floor, although bee abundance varied considerably among study years. Natural fruit‐set ratios were generally low but showed interannual variation. The annual fruit‐set ratios were positively correlated with annual bee abundance, thereby suggesting that under natural conditions, the orchid is pollinator‐limited. Given that C. amamiana is dependent exclusively on E. okinawae for pollination and is also probably pollinator‐limited, conservation plans for this orchid should take into consideration the welfare of long‐horned bees. In particular, as nectariferous flowers were unavailable within broad‐leaved forests, to satisfy the foraging requirements of long‐horned bees, conservation managers should be cognizant of conservation values of flowering plants distributed in sun‐lit sites near orchid habitats, and manage those in an appropriate manner.