bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upregulation of Hyaluronan-Mediated Motility Receptor Is Linked with Immune Infiltrates and Poor Prognosis in Lung Adenocarcinoma
Authors: Feng Siwei , Ma Wanli , Yang Bohan 
Published: April 2022

Abstract
Background
The hyaluronan-mediated motility receptor (HMMR) is excessive expression in a great deal of malignancies and has been implicated in cancer invasion and metastasis. Nevertheless, the prognostic values of HMMR in lung adenocarcinoma and its connection to immunological infiltrates are still unknown. As a result, we investigated the importance of HMMR in lung tumor prognosis and its relationship with immune infiltrates.
Methods
The Cancer Genome Atlas (TCGA) provided transcriptional profiling of HMMR in normal samples and pulmonary adenocarcinoma samples. The expression of the HMMR protein was analyzed by the Human Protein Atlas (HPA) and the Clinical Proteomic Tumor Analysis Consortium (CPTAC). To distinguish lung adenocarcinoma from normal tissues in the vicinity, we used the receiver operating characteristic (ROC) curve. Kaplan-Meier method was conducted to assess the effect of HMMR on survival. The STRING program created protein-protein interaction (PPI) networks. Functional enrichment analysis was carried out using the "ClusterProfiler" program. The association between HMMR mRNA immune infiltrates and expression was identified using tumor-immune system interaction database (TISIDB) and tumor immune estimate resource (TIMER).
Results
In lung adenocarcinoma tissues, the HMMR expression was substantially higher than in surrounding normal tissues. Lymphoma node metastases and high TNM stage were linked to increase HMMR mRNA expression. HMMR's specificity, sensitivity, and accuracy for distinguishing pulmonary cancer from neighboring controls were 86.2, 98.3, and 87.4 percent, respectively, at a cutoff level of 2.103, according to the ROC curve analysis. The prognosis for patients with high-HMMR lung adenocarcinoma was worse than for those with low-HMMR (41.9 vs. 59.9 months, P < 0.001), according to a Kaplan-Meier survival analysis. Correlation study revealed that the expression of HMMR mRNA was linked to immunological infiltrates and tumor purity.
Conclusion
In lung adenocarcinoma, immune infiltrates and poor survival are associated with upregulated HMMR. Our findings imply that HMMR may be utilized in lung adenocarcinoma as a predictor of poor prognosis and a possible biomarker for immune treatment.
The Efficacy of Tacrolimus Combined with Platelet Rich Plasma in Sjogren's Syndrome's Severe Dry Eye: A Randomized-Controlled Trial
Authors: Shams Majid , Barzegarpur Farzane , Akbari Zahra , Farsinejad Alireza 
Published: April 2022

Abstract
Purpose: Dry eye in Sjogren's ends up with ulcerations, infections, and even corneal perforations. Present treatments are mainly conservative. Due to the immunosuppressive effects of Tacrolimus drop and regenerative properties of platelet-rich plasma (PRP), this study aimed to evaluate the simultaneous use of these products in dry eye management.Methods: 20 cases of Sjogren's syndrome were included in this study. Patients were divided into three groups, the 'artificial tear drop,' Tacrolimus drops,' and 'Tacrolimus, and PRP drops'. The Schirmer's and TUBT tests and corneal fluorescein staining were assessed in each case following the determination of refractive errors and intraocular pressure, one week, one month, and three months after the initiation of therapy.Results: The majority of our cases were females, making up approximately 80%. Significant differences were found when assessing the level of irritation, and the results of the TUBT as well as fluorescein staining tests between the group treated with artificial teardrops and both of the other groups three months after treatment started. (P- values of 0.0005, 0.002 and 0.01, respectively) Schirmer's test, however, did not reveal significant levels (P-value = 0.5) when comparing the three treatment groups.Conclusion: Tacrolimus drop is a suitable and effective agent in severe cases of dry eye in Sjogren's syndrome, showing even more therapeutic benefits when given with PRP, based on the results of this study. Owing to this combination's increased anti-inflammatory and regenerative effects and its accelerating effects on the resolution of clinical symptoms, it can be considered superior to conventional therapies.
GWO based Atherosclerotic Cardiovascular Prediction model integrating Traditional and Non-Traditional parameters
Authors: paul Paulin , Shan B. Priestly 
Published: April 2022

Abstract
The study has proposed an optimized intelligent attribute reduction, risk prediction, and classification framework using Machine Learning for early identification of Atherosclerotic Cardiovascular (ASCV) risk status. Data preprocessing with normalization and missing data imputation using Multiple Imputation (MI) with Gaussian Copula (GC) method was done before optimal attribute selection. The proposed framework represented as SVMGWO-ANFISGWO-Multi-SVM has used Grey Wolf Optimization (GWO) algorithm. Feature selection was based on fitness evaluated wrapper-based multi-Support Vector Machine (multi-SVM). The optimal features selected by SVMGWO are processed by ANFISGWO for optimal ASCV risk prediction. Finally, multi-SVM is used to identify the risk class of the predicted ASCV risk. Performance of the study model were evaluated using Accuracy, Sensitivity, Specificity, Precision, G-mean, and F-measure features. Confusion and ROC plots are used to identify the classier performances. The Area Under Curve (AUC) was plotted to study the discrimination between the ASCV risk classes. The implementation was done in MATLAB 2015b. The ANFISGWO in the training phase has shown 80.7% accuracy, 100% sensitivity, 77.4% specificity, 43.3% precision, 60.2% G-mean, and 87.9% F-measure. Whereas, in the testing phase, the accuracy was 80%, 100% sensitivity, 77.1% specificity, 38.1% precision, 54.4% G-mean, and 87.7% F-measure. The overall AUC of 0.976 shows good discrimination between the ASCV risk classes.
Analysis of protein kinase C (HcPKC) gene expression and single-nucleotide polymorphisms related to inner shell color traits in Hyriopsis cumingii
Authors: Zhang Mengying , Chen Xiajun , Zhang Jinpan , Guo Baiying , Li Jiale , Bai Zhiyi 
Published: April 2022

Abstract
Background
Protein kinase C (PKC) is a multifunctional serine and threonine kinase that can regulate the activity of tyrosinase, and thereby regulate the synthesis and secretion of melanin. Hyriopsis cumingii is an important freshwater pearl mussel. In this study, we mainly study the role of PKC gene in the melanin synthesis pathway and its effect on the color of the nacre of H. cumingii.
Results
In this study, a HcPKC gene in H. cumingii was cloned and its effects on melanin synthesis and nacre color were studied. HcPKC was expressed in both purple and white mussels, and the level of mRNA expression was higher in the purple mussels than in white mussels. Strong and specific mRNA signals were detected in the dorsal epithelial cells of the mantle pallial layer, indicating that HcPKC may be involved in nacre formation. Single-nucleotide polymorphisms (SNP) associated with traits related to inner shell colors found that the A + 332G locus on the HcPKC gene was moderately polymorphic, and four other loci were low in polymorphism. There was strong linkage disequilibrium among the five loci. A haplotype was constructed and it was found that the frequency of T1 in the white population was significantly higher than that in the purple population (P < 0.05).
Conclusion
The study found that HcPKC of H. cumingii can be used as a candidate gene related to inner shell color, and some of the SNP sites can be used for molecular-assisted breeding in the spinnaker mussel, providing a reference for cultivating high-quality freshwater pearls.
Pedagogical content knowledge for teaching evolution across the primary school curriculum. A design study of a pre-service teacher education module
Authors: Hanisch Susan , Eirdosh Dustin 
Published: April 2022

Abstract
Background: In the last two decades, numerous studies have accumulated that indicate that evolutionary concepts can and should be taught at the primary school level. However, teaching evolution in primary school is presumably not yet the norm globally. At the same time, the educational potential of evolutionary concepts lies in their applicability to a large variety of (existing) curriculum topics in the natural and social sciences. Capitalizing on this generality of evolution requires broad teacher pedagogical content knowledge (PCK) about the teaching of evolutionary concepts. However, not much is known regarding ways to develop primary teacher PCK during pre-service training. In this article, we present the iterative design, implementation, and mixed methods evaluation (based on a design-based research framework) of a module concept for the development of pre-service primary school teacher PCK for teaching evolution as an interdisciplinary lens across the primary school curriculum.Results: Preliminary evidence indicates that participants were able to develop positive attitudes and motivations as well as core aspects of PCK regarding the teaching of evolutionary concepts in primary school. The resulting module design integrates existing collective PCK on teaching evolution at the primary level as well as novel design considerations and teaching approaches that can be integrated into existing pre-service teacher education programs. Conclusion: This study indicates that pre-service teachers can be enabled and motivated to teach evolutionary concepts across various themes in the primary school curriculum through a semester long course. However, challenges remain particularly regarding the integration of evolutionary concepts in mandated curriculum standards and teacher education contexts such that the educational potential of evolution can be fully capitalized on by teachers.
An Evaluation of the Coordinated Development of Coastal Zone Systems: A Case Study of China's Yellow Sea Coast
Authors: Fang Jian , Xu Min , Liu Baiqiao , Chen Zhou 
Published: April 2022

Abstract
The coastal zone is a zone of transition between the land and sea, and it contains rich natural resources and has high ecological value. It is also an important spatial unit for human beings to engage in production and life. In this study, the DEMATEL model was applied to identify the key factors influencing a complex coastal zone system. The results were then used to construct an indicator system used to evaluate the developmental stages of three coastal zone subsystems[ecological environmental (EE), social (SOC), and economic (ECO)]. The developmental levels of the EE, SOC, and ECO subsystems over the past 10 years were evaluated in five cities along the Yellow Sea coast of China, and the degrees of coupling and coordination among the subsystems were calculated using the coupling coordination degree model. The evaluation results showed that during the past decade, the EE, SOC, and ECO subsystem developmental levels increased in all five coastal regions, except for Yancheng, where there was a small decrease in the EE level, and the coupling degree of all five regions was high. Furthermore, the links and interactions between EE, SOC, and ECO were very close. The evaluation results relating to the coordination degree were relatively poor; the mean values of Qingdao and Nantong were at ‘Intermediate coordination level’, and the other three cities were at ‘Primary coordination’ or ‘Barely coordination level’. Some suggestions based on the multi-year development trend of subsystems and the coupling and coordination laws between them are proposed.
Effect of feeding sulphate reducing bacteria along with sulphur on growth performance, nutrient utilization and methane emission in goats
Authors: Uniyal Sandeep , Chaudhary Lal Chandra , Kala Anju , Agarwal Neeta , chaturvedi Vishu Bandhu 
Published: April 2022

Abstract
Competitive relationship exist between the sulphate reducing bacteria and the methanogens in the anaerobic environment including rumen for hydrogen where sulphate is not limiting for the growth and consequently inhibit the enteric methane emission as thermodynamically energetic of sulphate reduction (∆Go= -21.1 KJ/mole of H2) is more favourable than methanogenesis (∆Go= -16.9 KJ/mole H2). To validate this hypothesis a study was designed to investigate the effect of supplementation of sulphate reducing bacteria (SRB) as microbial feed additives alone or along with sulphur (as sodium sulphate) on methane production, live weight gain, feed intake, nutrient digestibility and energy metabolism in goats. The SRB used as microbial feed additives were isolated from goat rumen. The experiment was conducted on growing kids (n=36, 5-6 months of age) with average body weight of 10.08 ± 0.21 kg, divided in six groups (n=6). The six treatments were control fed basal diet (T1), SRB 0.5ml/kg BW (T2), sulphur (as sodium sulphate) 0.095% of DMI (total sulphur level in the diet 1.5 times the requirement) (T3), sulphur (as sodium sulphate) 0.095% of DMI + SRB 0.5ml/kg BW (T4), sulphur (as sodium sulphate) 0.19% of DMI (total sulphur level in the diet 2 times the requirement) (T5) and sulphur (as sodium sulphate) 0.19 % of DMI + SRB 0.5ml/kg BW (T6). Duration of study was 150 days and the goats were fed as per ICAR (2013) feeding standard. Methane (CH4) production (l/kg DMI) was reduced by 11.8 % (P=0.05) in T6 where sulphur (@ 0.19% DMI) was supplemented along with SRB4 (@ 0.5 ml/kg BW) as compared to T1 (un-supplemented group). However, the dry matter intake (DM), total weight gain (TG), average daily gain (ADG), feed conversion ratio (FCR), excretion of purine derivatives (allantoin, uric acid, xanthine and hypoxanthine) and digestibility of organic matter (OM), dry matter (DM), ether extract (EE), crude protein (CP), acid detergent fibre (ADF) and neutral detergent fibre (NDF) remained comparable (P>0.05) among all the groups. The experimental data revealed that feeding of SRB as an microbial feed additives along with sulphur (as sodium sulphate) is capable in reducing enteric CH4 emission without any adverse effect on rumen fermentation and digestibility of the nutrients.
LLIN Evaluation in Uganda Project (LLINEUP2) – Factors associated with coverage and use of long‑lasting insecticidal nets following the 2020-21 national mass distribution campaign: a cross-sectional survey of 12 districts
Authors: Okiring Jaffer , Gonahasa Samuel , Nassali Martha , Namuganga Jane F. , Bagala Irene , Maiteki‑Sebuguzi Catherine , Opigo Jimmy , Nabende Isaiah , Nangendo Joanita , Kabami Jane , Ssewanyana Isaac , Kiwuwa Steven M. , Nankabirwa Joaniter I. , Dorsey Grant , Briggs Jessica , Kamya Moses R. , Staedke Sarah G. 
Published: April 2022

Abstract
Background. In 2020-2021, long-lasting insecticidal nets (LLINs) were distributed nationwide in Uganda during the COVID-19 pandemic. A cross-sectional survey was conducted in 12 districts to evaluate the impact of the campaign 1-5 months after LLIN distribution. Methods. During April-May 2021, households were randomly selected from target areas (1-7 villages) surrounding 12 government-run health facilities established as Malaria Reference Centres; at least 50 households were enrolled per cluster. Outcomes included household ownership of LLINs distributed through the universal coverage campaign (UCC) (at least one UCC LLIN), adequate coverage of UCC LLINs (at least one UCC LLIN per 2 residents), and use of LLINs (resident slept under a LLIN the previous night). Multivariate logistic regression models were used to identify household- and individual-level factors associated with outcomes, controlling for clustering around health facilities.Results. In total, 634 households, with 3,342 residents and 1,631 bed-nets, were included. Most households (93.4%) owned at least 1 UCC LLIN, but only 56.8% were adequately covered by UCC LLINs. In an adjusted analysis, the factor most strongly associated with adequate coverage by UCC LLINs was fewer household residents (1-4 vs 7-14; adjusted odds ratio [aOR] 12.96, 95% CI 4.76-35.26, p<0.001; 5-6 vs 7-14 residents; aOR 2.99, 95% CI 1.21-7.42, p=0.018). Of the 3,166 residents of households that owned at least one UCC LLIN, only 1,684 (53.2%) lived in adequately covered households; 89.9% of these used an LLIN the previous night, compared to 1034 (69.8%) of 1,482 residents living in inadequately covered households. In an adjusted analysis, restricted to residents of inadequately covered households, LLIN use was higher in children under-five than those aged 5-15 years (aOR 3.04, 95% CI 2.08-4.46, p<0.001), and higher in household heads than distantly-related residents (aOR 3.94, 95% CI 2.38-6.51, p<0.001). Conclusions. Uganda’s 2021-21 campaign was successful, despite the COVID-19 pandemic. In future campaigns, strategies should be adopted to ensure high LLIN coverage, particularly for larger households. A better understanding of the drivers of LLIN use within households is needed to guide future interventions, educational messages, and behaviour change communication strategies; school-aged children and distantly-related residents appear vulnerable and could be targeted.
Sustainability consciousness, green innovation initiatives and CSR practices:A way forward for developing countries:
Authors: Chishti Mazhar , Bashir Rizwana , Safdar Sofia , Mancinelli Tanja 
Published: April 2022

Abstract
Green innovation hasn't gotten much attention as a factor of environmental policies in developing countries like Pakistan. Green innovation and long-term financial performance are very much dependent yet; despite the fact that many studies have looked into the influence of various corporate social responsibility (CSR) activities on environmental sustainability, few have looked into the implications of green innovation strategies and sustainability. From January to December 2021, data were collected from the respondent of 184 listed companies of 12 sectors of Pakistan Stock Exchange (PSX). The results were estimated by using partial least squares structural equation modeling (PLS-SEM). According to the findings of the study, all aspects of CSR were determined to be favorably significant in promoting environmentally sustainable growth except one aspect of CSR to consumers. Furthermore the mediation effect of green innovation is also aided by environmentally sustainable development.
Thermoacoustic sensor-based system for detection of respiratory diseases in chicken (Gallus domesticus L.)
Authors: Mugide Catherine , Ocen Gilbert Gilibrays , Omara Timothy , Lusiba Badru , Semwogerere Twaibu , Alunyu Andrew Egwar 
Published: April 2022

Abstract
Objective
Diseases such as avian influenza, infectious laryngotracheitis and Mycoplasma gallisepticum responsible for huge losses in poultry target the respiratory system of birds. The aim of this study was to design and assemble a system which can be used to detect respiratory diseases among chicken basing on the abnormal vocalization and temperature of chicken.
Results
The proposed system was assembled and operated in a way that the acoustic and thermal sensors acquire sound and temperature from the chicken environment, sends them to a microcontroller which then interprets the input and in case of abnormal sounds or temperature due to cough or snore, the system administrator and the farmer are notified. The results illustrated that the system could be used to improve the detection of respiratory disease outbreaks in chicken, which can potentially minimize losses in poultry farming.
Surgical approach could be a major factor in placenta Previa outcome: a retrospective study
Authors: Uwais Ala , Hijazin Marleen , Satari Anas , Al-abadleh Ahmed , Al-kharabsheh Ahlam , Abufraijeh Seham 
Published: April 2022

Abstract
Background:
Placenta previa carry high morbidity and mortality due to massive hemorrhage occur during surgery. So, there should be a standard surgical approach with less morbidity.
Methods:
This is a retrospective study that reviewed all deliveries in Al-karak governmental hospital between 2019–2022. Placenta previa cases were divided into two groups according to management. Group A was managed by incising the uterus at the level of fundus to avoid opening through the placenta, while Group B was managed by opening the lower uterine segment and delivering the baby through placenta after incising it.
Result:
This study included 26 cases of placenta previa, 12 of them (Group A) were managed by avoiding the placenta and the other 14 cases (Group B) were managed by opening through the placenta. There was no difference between the two groups in terms of age and the number of previous caesarean sections (table 1). Patients who underwent the suggested surgical approach (Group A) had less blood loss (Mdn = 775 cc) while Group B (Mdn = 1700 cc) U = 20.0 p = 0.001 was significantly higher in terms of blood loss. Similarly, the number of blood units given for Group B (Mdn = 5 units) was significantly higher than Group A (Mdn = 1 unit) U = 29.5. p = 0.005. lastly, the duration of hospital stay for Group A (Mdn = 2 days) was significantly less than Group B (Mdn = 6 days) U = 10.0. p = 0.000
Conclusion:
Incising the upper uterine segment to avoid the placenta may have better outcome regarding blood loss and its consequences.
Can we scale Digital Twins of Nature-based Solutions for stormwater and transboundary water security projects?
Authors: Brasil José Artur Teixeira , de Macedo Marina Batalini , de Oliveira Thalita Raquel Pereira , Ghiglieno Filippo Giovanni , de Souza Vladimir Caramori Borges , Marinho e Silva Gabriel , Gomes Júnior Marcus Nóbrega , de Souza Felipe Augusto Arguello , Mendiondo Eduardo Mario 
Published: April 2022

Abstract
Nature-based Solutions (NbS) are presented as an alternative and decentralized solutions with different application scales for problems addressed to urban expansion as water quality reduction and floods. The usage of control strategies and mathematical modeling techniques has shown promising results for optimizing hydraulic and water treatment processes. The Digital Twins (DT) as process integration technology are widely used in industry, and recently these technique usages in urban water systems are showing effective results in both management and planning. However, there is a lack of proper literature definition for DT applied to NbS, especially for stormwater and transboundary water security projects. Thus, this paper sought through a literature review to access the existing conceptual challenges and the DT definition as a framework, identify how the mathematical modeling reported in the literature can improve the DT development, and evaluate the potential benefits associated with the application of DT in NbS.