bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tunable control of insect pheromone biosynthesis inNicotiana benthamiana
Authors: Kallam Kalyani , Moreno-Giménez Elena , Mateos-Fernández Ruben , Tansley Connor , Gianoglio Silvia , Orzaez Diego , Patron Nicola J. 
Published: June 2022

SummaryPrevious work has demonstrated that plants can be used as production platforms for molecules used in health, medicine, and agriculture. Production has been exemplified in both stable transgenic plants and using transient expression strategies. In particular, species ofNicotianahave been engineered to produce a range of useful molecules, including insect sex pheromones, which are valued for species-specific control of agricultural pests. To date, most studies have relied on strong constitutive expression of all pathway genes. However, work in microbes has demonstrated that yields can be improved by controlling and balancing gene expression. Synthetic regulatory elements that provide control over the timing and levels of gene expression are therefore useful for maximizing yields from heterologous biosynthetic pathways. In this study, we demonstrate the use of pathway engineering and synthetic genetic elements for controlling the timing and levels of production of Lepidopteran sex pheromones inNicotiana benthamiana. We demonstrate that copper can be used as a low-cost molecule for tightly regulated inducible expression. Further, we show how construct architecture influences relative gene expression and, consequently, product yields in multigene constructs. We compare a number of synthetic orthogonal regulatory elements and demonstrate maximal yields from constructs in which expression is mediated by dCas9-based synthetic transcriptional activators. The approaches demonstrated here provide new insights into the heterologous reconstruction of metabolic pathways in plants.
How does oil price uncertainty affect output in the Central and Eastern European economies? – the Bayesian-based approaches
Authors: Živkov Dejan , Đurašković Jasmina 
Published: June 2022

PurposeThis paper aims to investigate how oil price uncertainty affects real gross domestic product (GDP) and industrial production in eight Central and Eastern European countries (CEEC).Design/methodology/approachIn the research process, the authors use the Bayesian method of inference for the two applied methodologies – Markov switching generalized autoregressive conditional heteroscedasticity (GARCH) model and quantile regression.FindingsThe results clearly indicate that oil price uncertainty has a low effect on output in moderate market conditions in the selected countries. On the other hand, in the phases of contraction and expansion, which are portrayed by the tail quantiles, the authors find negative and positive Bayesian quantile parameters, which are relatively high in magnitude. This implies that in periods of deep economic crises, an increase in the oil price uncertainty reduces output, amplifying in this way recession pressures in the economy. Contrary, when the economy is in expansion, oil price uncertainty has no influence on the output. The probable reason lies in the fact that the negative effect of oil volatility is not strong enough in the expansion phase to overpower all other positive developments which characterize a growing economy. Also, evidence suggests that increased oil uncertainty has a more negative effect on industrial production than on real GDP, whereas industrial share in GDP plays an important role in how strong some CEECs are impacted by oil uncertainty.Originality/valueThis paper is the first one that investigates the spillover effect from oil uncertainty to output in CEEC.
The impact of architectural design studio education on perceptions of sustainability
Authors: Calikusu Ayca Nilufer , Cakmakli Aysem Berrin , Gursel Dino Ipek 
Published: June 2022

PurposeSince architectural design studio education has critical importance on the students' knowledge, skills, and awareness of sustainability, this study presents a point of view about relations between architectural education and social, economic, environmental sustainability perception of students within the scope of design studios. The primary purpose is to investigate the role of design studio education in the context of sustainability.Design/methodology/approachIn the study, the Department of Architecture in Middle East Technical University (METU) was selected as a case study. The impact of design studio education on the understanding of sustainability of architecture students was investigated by conducting a survey with students and in depth-interviews with volunteer lecturers. In quantitative research after formulating four hypotheses, the questionnaire was carried out with 237 architecture students from different years. Then by using the thematic analysis method, the qualitative part of the study was conducted with 11 lecturers.FindingsFindings reveal that the design studio education and theoretical lectures on sustainability are effective in improving students' sustainability understanding.Originality/valueArchitectural education supports the students about comprehensive understanding and knowledge of national and global issues. This article conducted a case study to investigate the impact of the design studio on the perception of sustainability. Data were collected from architecture students and volunteer lecturers of METU between November 2018 and February 2019. The research is original examining the importance of architectural studio education in the development of knowledge, skills, and awareness of sustainability.
Lean-offsite-simulation nexus for housing construction: a state-of-the-art review of the existing knowledge
Authors: Daniel Emmanuel Itodo , Oshodi Olalekan 
Published: June 2022

Purpose
The purpose of this study is to present an overview of the existing knowledge on the combined application of lean, off-site and simulation (LOS) in housing delivery.

Design/methodology/approach
A systematic literature review approach was adopted. Based on a comprehensive search using Scopus, Web of Science and the International Group for Lean Construction databases, 66 relevant journal articles were identified and analysed.

Findings
This study found that the most significant impacts of the combined application of LOS in housing delivery are the capacity to visualise the production processes as a whole in real time, exposure and removal of non-value-adding activities from the production and faster delivery. However, the combined application of LOS is low compared to a single application of each technique in housing delivery.

Practical implications
The results provide relevant stakeholders and actors in the housing sector (private and public housing developers, off-site housing manufacturers and constructors, housing associations and government housing agencies, among others) with the information needed to improve the outcomes of housing delivery through the application of LOS.

Originality/value
This study contributes to the ongoing debate on addressing the global housing shortage by presenting an integrated overview of the existing knowledge on the impact of the nexus of LOS and providing compelling evidence for its usage in housing delivery. It also demonstrates how the combined application of LOS supports the achievement of the flow and value view in the transformation flow value model, which was not previously reported.
Owner-based benefit evaluation of BIM applications in China's engineering projects
Authors: Li Xiaojuan , Zhang Zhou , Jim C.Y. , Lai Jiyu , Chen Xueqing 
Published: June 2022

PurposeThis paper aims to establish a model to evaluate the benefits of building information modeling (BIM) from the owners' perspective. The model analyzes the correlation between five secondary benefit indicators and their subsystems: product, financial, organizational, management and strategic. The final key factors of BIM benefits provide a decision-making basis for owners to raise the efficiency of BIM application.Design/methodology/approachFirstly, the authors combed 31 BIM-related literature and interviewed experts to identify 15 preliminary benefit indicators. The authors established a BIM benefit evaluation system based on relevant concepts, including two primary indicators, five secondary indicators and 15 tertiary indicators. Secondly, the authors analyzed the indicators by the extension theory of matter element analysis and a questionnaire survey of expert opinion. Finally, the new method was applied to a case study of a large shopping center in east China for empirical verification.FindingsA BIM benefit evaluation model, including a three-tiered hierarchy of primary, secondary and tertiary indicators, was constructed through literature review and expert opinions. The model determined the critical factors of BIM benefits, enhanced understanding of owner benefits and improved BIM application under the owners' leadership.Originality/valueAt present, most studies focus on specific project stages or benefit indicators. This study developed an integrated BIM benefit evaluation system that targets owners. The findings could foster the development of China's construction industry, promote owner-led BIM application and advocate adopting the benefit evaluation method.
Modelling organizational resilience structure: insights to assess resilience integrating bounce-back and bounce-forward
Authors: Bartuseviciene Ilona , Butkus Mindaugas , Schiuma Giovanni 
Published: June 2022

PurposeThis paper aims to model organizational resilience structure. Based on the central insights of the scientific literature, organizational resilience is modelled as the result of an organizational capacity to bounce-back and bounce-forward.Design/methodology/approachThe paper is based on a quantitative empirical study to support the structural perspective of organizational resilience and investigate the relationships among the dimensions to test the above hypothesis by applying confirmatory factor analysis (CFA) and structural equation modelling (SEM) methods.FindingsThe results confirmed three models that could be adopted to assess organizational resilience. The first model endorsed endogenous positive interrelationship among all three dimensions. The second model indicated that bounce-back dimensions, i.e. network and leadership and culture, have endogenous effects. Only the leadership and culture dimension is positively associated with a bounce-forward, i.e. change-ready and learning dimension. And the third model demonstrated that the network dimension is linked to leadership and culture, which is linked to the change ready and learning dimension.Originality/valueThis study attempts to provide empirical evidence identifying the links between the bounce-back and bounce-forward stages of organizational resilience. These results contribute to the development of organizational resilience theory, confirming the conceptual statements that resilience is the ability to return to the routine and to adapt to the changing environment by overcoming dynamic events, stressing the idea of the importance of enhanced learning capacity, which allows for growth by constantly learning from oneself by gaining unique experiences.
Aspiring and inspiring: the role of women in educational leadership
Authors: Mareque Mercedes , de Prada Elena , Pino Juste Margarita 
Published: June 2022

Purpose
This study aims to delve deep into the differences observed in leadership acquisition and development between men and women and analyse the impact of certain extra-curricular activities demonstrated to influence leadership acquisition. With this evidence, this study intends to detect areas that require deeper analysis and suggest actions to redress the balance and promote a more egalitarian leadership in education in both teaching and management positions.

Design/methodology/approach
Accidental, as opposed to random, sampling was used via a cross-curricular study with a quasi-experimental design. The participants in this study were 335 teacher training students in Spanish universities. The Teamwork Skills Questionnaire was used to evaluate leadership and a self-assessment questionnaire to measure extracurricular activities.

Findings
Female students score lower in leadership skills and are engaged in fewer activities associated with successful leadership skills development. Very few students receive any training on leadership, with even fewer female than male students. Furthermore, the practice of certain extra-curricular activities can boost leadership skills. It has also been noted that these activities are carried out in different ways, depending on student gender.

Originality/value
This study has two points of focus: the level of leadership among education students, differentiating between male and female students, and the impact of certain extra-curricular activities carried out during the academic year, which have proven to have a considerable effect on the development of leadership skills.
University student experiences of work-based placements during COVID-19 pandemic: an inter-disciplinary survey of allied health and social work students
Authors: Quigley Duana , Poole Claire , Whiting Sinead , O'Connor Erna , Gleeson Claire , Alpine Lucy 
Published: June 2022

PurposeWork-based placements are central to the university education of allied health and social work (AHSW) students. As a result of COVID-19, the clinical learning environment of students' work-based placements was dramatically altered resulting in numerous documented challenges. This inter-disciplinary study aimed to evaluate AHSW students' perceptions and experiences of completing a diverse range of work-based placements during COVID-19.Design/methodology/approachThis study was a mixed-method inter-disciplinary study using an anonymous online survey consisting of multiple choice, Likert scale and free text questions. Mixed-methods design supported amalgamation of insights from positivism and interpretivism perspectives and enabled research questions to be answered with both breadth and depth. 436 students were invited to participate who were enrolled in five AHSW educational university programmes: speech and language therapy, occupational therapy, physiotherapy, radiation therapy and social work. Data collected was analysed using both quantitative (descriptive and analytical statistics) and qualitative (thematic analysis) methods.Findings118 students participated (response rate: 27%) representing a range of AHSW disciplines who attended diverse placement settings. While there was extensive disruption in the learning environment leading to increased levels of stress and concern, a triad of individual and systemic supports helped to ensure positive work-based placement experiences and student success for the majority of AHSW students during COVID-19: (1) university preparation and communication; (2) placement site and supervisor support; and (3) students' resilience and capacity to adapt to a changed work-place environment.Originality/valueThis inter-disciplinary study reports the work-based placement experiences from the professional education programmes of healthcare students during the COVID-19 pandemic, giving a unique view of their perspectives and learning during this unprecedented crisis.
Positive and negative switching barriers: promoting hotel customer citizenship behaviour through brand attachment
Authors: Lee Chang-Hun , Kim Hye-Rhim 
Published: June 2022

Purpose
This study aims to develop and test a theoretical model postulating that a hotel customer’s brand attachment is reinforced by positive and negative switching barriers, which, in turn, determine customer citizenship behaviour (CCB) towards hotel brands.

Design/methodology/approach
Surveys were conducted and completed by 233 respondents in the USA who had favourite hotel brands and used these brands in the previous year. A framework was developed based on the literature, and eight hypotheses were tested using structural equation modelling.

Findings
The findings suggest that a customer’s brand attachment (brand-self connection and brand prominence) to a hotel is strengthened not only by relational benefits (positive switching barriers) but also by switching costs (negative switching barriers). Brand prominence can promote CCB, whereas the impact of brand-self connection on CCB is rather limited.

Research limitations/implications
This study highlights the importance of affirmative and passive reasons for customers to remain in a relationship with the hotel brand and how sub-dimensions of switching barriers are interrelated to predict a customer’s attitude and behaviour to the brand. By emphasising the role of customers’ hotel brand attachment, this study also ascertains that cognitive and affective bonds towards a hotel brand can be significant antecedents to their extra-role behaviours.

Originality/value
This research contributes to the hospitality literature by expanding the realm of consumer behaviour research on switching barriers, brand attachment and CCB.
Managing complementors in innovation ecosystems: a typology for generic strategies
Authors: Liang Xingkun , Luo Yining , Shao Xiaolin , Shi Xianwei 
Published: June 2022

PurposeInnovation ecosystem research has highlighted complementors as the critical force to determining focal firm innovation’s success in addition to the traditional value chain or supply chain perspective. However, literature is relatively scarce in terms of innovation ecosystem governance, especially, on how to manage various types of complementors. The purpose of this paper is to fill this theoretical gap by developing a typology of managing complementors from multiple case studies.Design/methodology/approachThis study conducted multiple case studies of three leading focal firms with ecosystem strategies to understand innovation ecosystem governance. Theoretical themes are inductively generated to reveal their success in managing complementors in their ecosystems.FindingsThe case analysis reveals four generic strategies to manage complementors. These strategies are contingent on the types of complementors and level of interdependence: focal firms tend to engage functional complementors and collaborate with infrastructural complementors when the level of interdependence is higher, and acquire functional complementors and nurture infrastructural complementors when the level of interdependence is lower.Practical implicationsFor practitioners, this study can improve their understanding on the mechanisms of innovation ecosystem governance, particularly interdependence between participants in an innovation ecosystem, and developing appropriate strategies to manage different types of complementors in innovation ecosystems.Originality/valueThis study contributes to innovation ecosystem literature by enriching the conceptualization of interdependence in innovation ecosystems and unpacking innovation ecosystem governance with the inductively developed holistic typology of strategies to manage complementors. Meanwhile, this study also suggests underlying mechanisms for how innovation ecosystem governance and, therefore, contributes to a systematic theory on understanding innovation ecosystem governance.
Battery R&D decision of electric vehicle manufacturer considering government subsidy
Authors: Ke Hua , Chen Xingyue 
Published: June 2022

PurposeIn this paper, the authors aim to consider the manufacturer's battery research and development (R&D) decision under subsidy. The supply chain includes two manufacturers, which produce substitutable electric vehicles, and a battery supplier. One of the manufacturers can choose to develop batteries or buy batteries. The authors assume consumers do not have enough trust in the manufacturer-made battery.Design/methodology/approachStackelberg game is made use of to study the battery R&D strategy of the manufacturer under the incentive of government subsidies. This paper makes a comparative analysis on six situations, then the authors get some conclusions and give some managerial insights.FindingsThe results show that subsidy strategies do not necessarily reduce actual payments when the manufacturer does not research and develop batteries. The retail prices and actual payments are closely related to the substitutability and total cost advantage of product. The authors also find consumer trust positively affects the demand of the electric vehicles using the manufacturer-made batteries and then affects the manufacturer's battery R&D decision. When consumers have low trust in manufacturer-made battery, subsidy can bring greater sales and make R&D more profitable than procurement, so that the manufacturer chooses R&D. This study's findings also suggest consumer subsidy is always better for the government.Originality/valueDistinguished from previous studies, the authors discuss the decision-making of component research, and introduce various government subsidy strategies and consumer trust to study their roles in the manufacturer's battery R&D choice.
The moderating role of age in the relationship between perceived over qualification, employability, job insecurity and global psychological needs satisfaction
Authors: Sabino Ana , Basilio Elizabete , Lopes Silvia , Chambel Maria José , Dias Paulo C. , Cesário Francisco 
Published: June 2022

Purpose
This study aims to examine the relationship between perceived overqualification, perceived employability, perceived job insecurity and global psychological needs satisfaction, considering the moderating role of age groups.

Design/methodology/approach
A cross-sectional survey design with a sample of 350 individuals was used in this study.

Findings
A cross-sectional survey design with a sample of 350 individuals was used in this study.

Originality/value
By addressing the moderating role of age, this study is original and has practical implications for age diversity management.
Sclerotherapy versus percutaneous catheter drainage for treatment of post‐mastectomy seroma: How to select?
Authors: Awad Joseph Rizk , Alkilany Mohamed M. , Abdelhamid Mohamed I. 
Published: June 2022

AbstractBackgroundPost‐mastectomy seroma is a well‐known complication which may be a seat for further complications as infection and flap necrosis, or it may cause delay in the adjuvant therapy. Several methods are used now in its prevention and treatment. This opened the door for on‐going evaluation of these methods as in this article.MethodsThis is an interventional study aiming to define predictive factors for selecting the most appropriate treatment modality for post‐mastectomy seroma. Female patients with established diagnosis of post‐mastectomy seroma following modified radical mastectomy, were treated by local injection of tetracycline after the seroma fluid was aspirated, then a crepe bandage was applied over the mastectomy area. Then, after 5 days the patients were examined again for seroma re‐collection and, at this time, percutaneous catheter drainage was applied.ResultsEighteen patients were submitted to this study where multiple factors were studied regarding their effect on the response gained by local tetracycline injection (and can predict its failure with the need for percutaneous catheter application) like; patient age, physical activities, comorbidities, tumour size and pathological type, total leukocytic count, serum albumin, and the volume of the amount aspirated at the first session. Both serum albumin and the volume of the amount aspirated at the first session showed significant predictive effect (p‐value; 0.019, <0.00001 respectively).ConclusionsBoth serum albumin and the volume of the amount aspirated at the first session are efficient predictors for deciding which treatment modality can be selected.