bluuebunny/crossref_metadata_2025_split huggingface dataset:
Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation
Authors: Bowen Andrew J. , Ekbom Dale C. , Hunter Danielle , Voss Stephen , Bartemes Kathleen , Mearns‐Spragg Andrew , Oldenburg Michael S. , San‐Marina Serban 
Published: September 2022

AbstractObjectives/HypothesisCompare proteomic profiles of rabbit vocal folds (VFs) injected with micronized cross‐linked jellyfish collagen “collagen Type 0” (MX‐JC) against two clinical products for injection medialization laryngoplasty (IL).Study DesignAnimal model.MethodsLeft recurrent laryngeal nerve sectioning and IL were performed in New Zealand White rabbits (N = 6/group). Group 1 received (MX‐JC) and adipose‐derived stem cells (ADSCs), Group 2, MX‐JC alone; Group 3, cross‐linked hyaluronic acid; and Group 4, micronized acellular dermis. Animals were sacrificed at 4 and 12 weeks. Proteomic profiling of injected versus noninjected VFs by nano‐liquid chromatography, tandem mass spectrometry, and reactome gene ontology analysis was performed.ResultsOverall, 37–61 proteins were found to be upregulated and 60–284 downregulated in injected versus non‐injected VFs (>1.5 fold, false discovery rate‐adjusted p < .05). Over‐representation analysis (% of total) revealed top up‐regulated pathways at 4 and 12 weeks, respectively: Group 1, keratan sulfate metabolism (46%) and cellular processes (29%); Group 2, extracellular matrix (ECM)/collagen processes (33%) and beta oxidation (39%); Group 3, cellular processes (50%) and energy metabolism (100%); and Group 4, keratan sulfate metabolism (31%) and inflammation (50%). Top downregulated pathways were: Group 1, Inflammation (36%) and glucose/citric acid metabolism (42%); Group 2, cell signaling (38%) and glucose/citric acid metabolism (35%); Group 3, keratan sulfate metabolism (31%) and ECM/collagen processes (48%); and Group 4, glucose/citric acid metabolism (33%) and ECM/collagen processes (43%).ConclusionsMX‐JC “collagen Type 0” upregulates pathways related to ECM/collagen formation and downregulates pathways related to inflammation suggesting that it is promising biomaterial for IL.Level of EvidenceNA
Understanding corallite demography to comprehend potential bias in sclerochronology: Analysis of coral modular growth by micro‐computed tomography
Authors: Medellín‐Maldonado Francisco , López‐Pérez Andrés , Ruiz‐Huerta Leopoldo , Carricart‐Ganivet Juan P. 
Published: September 2022

AbstractThe discovery of alternating growth bands in the skeletons of massive corals led to the possibility of using them as environmental indicators. However, skeleton formation is the result of the growth of thousands of polyps depositing millimeter‐sized CaCO3structures, called corallites. Nevertheless, the orientation of the corallite trajectories and their position with respect to the colony could be altering the information obtained from the skeleton. In this sense, to obtain reliable information from coral skeletons, it is necessary to understand how polyp population growth influences coral growth rates. For this reason, we present a study that combines computed tomography image segmentation, optical densitometry, and demographic equations to follow the trajectory of corallites in order to model coral growth from the replication of its corallites and understand the responses in terms of their growth. We observed that both corallite replication and skeletal growth characteristics vary significantly according to the position they occupy within the colony. The central zone of the colony promotes corallite replication, and it is in this zone that we measured the highest values of extension and calcification. These variations in vegetative and skeletal growth are possibly in accordance with the variability of available resources and environmental stimuli in different zones of the skeleton. This approach will allow us to explore future lines of research associated with the size limits of different coral species and to observe how different drivers modulate polyp budding.
Oxygenation of a karst subterranean estuary during a tropical cyclone: mechanisms and implications for the carbon cycle
Authors: Brankovits David , Pohlman John W. , Lapham Laura L. 
Published: September 2022

AbstractSeasonal precipitation affects carbon turnover and methane accumulation in karst subterranean estuaries, the region of coastal carbonate aquifers where hydrologic and biogeochemical processes regulate material exchange between the land and ocean. However, the impact that tropical cyclones exert on subsurface carbon cycling within karst landscapes is poorly understood. Here, we present 5‐month‐long hydrologic and chemical records from 1 and 2 km inland from the coastline within the Ox Bel Ha Cave System in the northeastern Yucatan Peninsula. The record encompasses wet and dry seasons and includes the impact of rainfall during the development of Tropical Storm Hanna in October 2014. Methane accumulated in highest concentrations at the inland site, especially during the wet season preceding the storm. Intense rainfall led to episodic increases in water level and salinity shifts at both sites, indicating a spatially widespread hydrologic response. The most profound storm effect was a ~ 0.8 mg L−1 pulse of dissolved oxygen that declined to zero within 2 weeks and corresponded with a reduction of methane. A positive shift in methane's stable carbon isotope content from −62.6‰ ± 0.6‰ before the storm to −44.0‰ ± 2.4‰ after the storm indicates microbial methane oxidation was a mechanism for the loss of groundwater methane. Post‐storm methane concentrations did not recover to pre‐storm levels during the observation period, suggesting tropical cyclones have long‐lasting (months) effects on the carbon cycle. Compared to seasonal effects, mixing and oxygen inputs during storm‐induced hydrologic forcing have an outsized biogeochemical influence within stratified coastal aquifers.
Electrochemical study of Al–Zn–Mg–Cu alloy during deep cryogenic process
Authors: Zhang Weijian , Wang Lei , Su Ruiming , Li Guanglong , Qu Yingdong 
Published: September 2022

AbstractAl–Zn–Mg–Cu alloy was treated by deep cryogenic treatment combined with T6 treatment to obtain excellent properties. The hardness of the alloy was measured by a Vickers hardness tester. The corrosion behaviors of the alloy were monitored by electrochemical techniques and scanning electron microscopy. Furthermore, the microstructures of the alloy were examined by transmission electron microscope. The results show that after deep cryogenic treatment, the hardness and corrosion resistance of the alloy will be optimized, and the corrosion resistance and hardness will be strongly affected by the precipitate state. When the distance between grain boundary precipitates becomes larger and discontinuous, the corrosion resistance will be significantly improved. When the amount of matrix precipitates is large, the fine dispersion distribution can well increase the hardness. The best combination of the hardness and corrosion resistance is exhibited for the alloy treated at −196°C for 10 h with aging treatment at 120°C for 16 h, the hardness, the intergranular corrosion depth, the current density, and the corrosion rate are 189.6 HV, 31.8 μm, 0.0019 mA/cm2, and 0.0022 mm/a, respectively.
Characterization and corrosion evaluation of high‐entropy TixNb0.5MnMo0.5Zr0.3 (x = 0.5, 0.75, 1) thin films for biomedical applications
Authors: Sanaei Saber , Atapour Masoud , Kermanpur Ahmad 
Published: September 2022

AbstractIn the present study, high‐entropy TixNb0.5MnMo0.5Zr0.3 (x = 0.5, 0.75, 1) thin films with excellent corrosion behavior were deposited on AISI 316L using direct current magnetron sputtering. The deposited films were structurally characterized using X‐ray diffraction and field‐emission scanning electron microscopy. The results confirmed the formation of an face‐centered cubic structure with a uniform distribution of constituent elements. It was shown that the crystal structure of thin films was independent of the Ti content. The corrosion properties of the films were evaluated using electrochemical impedance spectroscopy (EIS) and polarization examinations at 37°C in the simulated body fluid electrolyte. Based on the EIS results, the stability range of the passive layer was improved by increasing the Ti content. The potentiodynamic polarization curves demonstrated that the film with a higher Ti content had the lowest icorr, indicating more corrosion resistance than other films with a lower Ti content and substrate. Another reason for the superior corrosion performance of the thin film with a higher amount of Ti was correlated with its quasi‐columnar structure. In general, the improvement of the corrosion behavior of the thin films can be associated with the chemical composition and microstructure formed during the deposition process.
Impact of nano‐SiCP and nano‐hBNP on the corrosion performance and fatigue behavior of Mg–Zn hybrid nanocomposites
Authors: Krishnan Parthiban , Lakshmanan Poovazhagan , Annamalai Gnanavelbabu 
Published: September 2022

AbstractMagnesium (Mg) alloys are characterized by their high specific strength, strong damping resistance, and poor performance against corrosion. This study attempted to evaluate the corrosion capability and fatigue behavior of Mg–Zn alloys reinforced with nanosized silicon carbide (SiC) and hBN particles. Initially, different combinations (1.0 wt% nano‐SiCP [fixed] and 0.5, 1.0, and 1.5 wt% nano‐hBNP) of hybrid nanocomposites were synthesized through a stir‐ultrasonication casting process. The samples cut from the nanocomposites were subjected to salt spray, potentiodynamic polarization, tensile, and fatigue tests. The resistance to corrosion and fatigue characteristics of the monolithic alloy and hybrid nanocomposites were measured and compared. Due to the reduction in potential imbalance across the Mg‐α and I‐phases, the hybrid nanocomposites revealed enhanced corrosion performance as compared with the base alloy. The fatigue strength of the Mg–Zn alloy reinforced with 1% SiCP and 1.5% hBNP is about 94 MPa, which is 41% greater than the base alloy's fatigue strength. The scanning electron microscopy micrograph is helpful in investigating the microstructures of the corrosion, tensile, and fatigue‐tested samples.
Different outer membrane c‐type cytochromes are involved in direct interspecies electron transfer to Geobacter or Methanosarcina species
Authors: Holmes Dawn E. , Zhou Jinjie , Smith Jessica A. , Wang Caiqin , Liu Xinying , Lovley Derek R. 
Published: September 2022

AbstractDirect interspecies electron transfer (DIET) may be most important in methanogenic environments, but mechanistic studies of DIET to date have primarily focused on cocultures in which fumarate was the terminal electron acceptor. To better understand DIET with methanogens, the transcriptome of Geobacter metallireducens during DIET‐based growth with G. sulfurreducens reducing fumarate was compared with G. metallireducens grown in coculture with diverse Methanosarcina. The transcriptome of G. metallireducens cocultured with G. sulfurreducens was significantly different from those with Methanosarcina. Furthermore, the transcriptome of G. metallireducens grown with Methanosarcina barkeri, which lacks outer‐surface c‐type cytochromes, differed from those of G. metallireducens cocultured with M. acetivorans or M. subterranea, which have an outer‐surface c‐type cytochrome that serves as an electrical connect for DIET. Differences in G. metallireducens expression patterns for genes involved in extracellular electron transfer were particularly notable. Cocultures with c‐type cytochrome deletion mutant strains, ∆Gmet_0930, ∆Gmet_0557 and ∆Gmet_2896, never became established with G. sulfurreducens but adapted to grow with all three Methanosarcina. Two porin–cytochrome complexes, PccF and PccG, were important for DIET; however, PccG was more important for growth with Methanosarcina. Unlike cocultures with G. sulfurreducens and M. acetivorans, electrically conductive pili were not needed for growth with M. barkeri. Shewanella oneidensis, another electroactive microbe with abundant outer‐surface c‐type cytochromes, did not grow via DIET. The results demonstrate that the presence of outer‐surface c‐type cytochromes does not necessarily confer the capacity for DIET and emphasize the impact of the electron‐accepting partner on the physiology of the electron‐donating DIET partner.
A deep‐sea sulfate‐reducing bacterium generates zero‐valent sulfur via metabolizing thiosulfate
Authors: Liu Rui , Shan Yeqi , Xi Shichuan , Zhang Xin , Sun Chaomin 
Published: September 2022

AbstractZero‐valent sulfur (ZVS) is a crucial intermediate in the sulfur geobiochemical circulation and is widespread in deep‐sea cold seeps. Sulfur‐oxidizing bacteria are thought to be the major contributors to the formation of ZVS. However, ZVS production mediated by sulfate‐reducing bacteria (SRB) has rarely been reported. In this study, we isolated and cultured a typical SRB designated Oceanidesulfovibrio marinus CS1 from deep‐sea cold seep sediment in the South China Sea. We show that O. marinus CS1 forms ZVS in the medium supplemented with thiosulfate. Proteomic and protein activity assays revealed that thiosulfate reductase (PhsA) and the sulfide:quinone oxidoreductase (SQR) played key roles in driving ZVS formation in O. marinus CS1. During this process, thiosulfate firstly was reduced by PhsA to form sulfide, then sulfide was oxidized by SQR to produce ZVS. The expressions of PhsA and SQR were significantly upregulated when O. marinus CS1 was cultured in a deep‐sea cold seep, strongly indicating that strain CS1 might form ZVS in the deep‐sea environment. Notably, homologs of phsA and sqr were widely identified from microbes living in sediments of deep‐sea cold seep in the South China Sea by the metagenomic analysis. We thus propose that SRB containing phsA and sqr genes potentially contribute to the formation of ZVS in deep‐sea cold seep environments.
Suppression of Invasive Fish in the West: Synthesis and Suggestions for Improvement
Authors: Klein Zachary B. , Quist Michael C. , Guy Christopher S. 
Published: September 2022

Abstract
Reservoirs are ubiquitous features on the landscape of the western United States. Although reservoirs provide numerous benefits (e.g., irrigation, flood control, hydropower, recreational use), these systems are often a concern from an ecological perspective. Reservoirs support fisheries primarily composed of nonindigenous sport fishes that may become invasive and negatively influence recipient ecosystems. Furthermore, reservoirs alter adjacent riverine habitats, further increasing the threat of invasive fishes to aquatic systems. As such, most western natural resource management agencies focus considerable effort on managing the threat of invasive fish species. Unfortunately, controlling invasive fish is expensive and rarely effective because of a lack of clear objectives, appropriate fishing mortality, and long-term commitment. In an effort to improve management of invasive fish in the western United States, we reviewed existing literature to identify the steps necessary to effectively suppress these species. Specifically, we provide guidance on defining achievable objectives, assessing feasibility, evaluating success, and improving the efficiency of invasive fish suppression. This iterative approach provides managers with a framework to effectively address the challenge of suppressing invasive fish in the western United States.
Fibrosis imaging with multiparametric proton and sodium MRI in pig injury models
Authors: Rasmussen Camilla W. , Bøgh Nikolaj , Bech Sabrina K. , Thorsen Thomas H. , Hansen Esben S. S. , Bertelsen Lotte B. , Laustsen Christoffer 
Published: September 2022

AbstractChronic kidney disease (CKD) is common and has huge implications for health and mortality. It is aggravated by intrarenal fibrosis, but the assessment of fibrosis is limited to kidney biopsies, which carry a risk of complications and sampling errors. This calls for a noninvasive modality for diagnosing and staging intrarenal fibrosis. The current, exploratory study evaluates a multiparametric MRI protocol including sodium imaging (23Na‐MRI) to determine the opportunities within this modality to assess kidney injury as a surrogate endpoint of fibrosis. The study includes 43 pigs exposed to ischemia–reperfusion injury (IRI) or unilateral ureteral obstruction (UUO), or serving as healthy controls. Fibrosis was determined using gene expression analysis of collagen. The medulla/cortex ratio of 23Na‐MRI decreased in the injured kidney in the IRI pigs, but not in the UUO pigs (p = 0.0180, p = 0.0754). To assess the combination of MRI parameters in estimating fibrosis, we created a linear regression model consisting of the cortical apparent diffusion coefficient, ΔR2*, ΔT1, the 23Na medulla/cortex ratio, and plasma creatinine (R2 = 0.8009, p = 0.0117). The 23Na medulla/cortex ratio only slightly improved the fibrosis prediction model, leaving 23Na‐MRI in an ambiguous place for evaluation of intrarenal fibrosis. Use of multiparametric MRI in combination with plasma creatinine shows potential for the estimation of fibrosis in human kidney disease, but more translational and clinical work is warranted before MRI can contribute to earlier diagnosis and evaluation of treatment for acute kidney injury and CKD.
Aspects of emotional labor of public health nurses engaged in interpersonal support
Authors: Honda Junko , Sasaki Akiko , Tsuda Shio , Matsuyama Machiko , Morita Kumiko 
Published: September 2022

AbstractAimClarifying the emotional labor of public health nurses while providing interpersonal support.DesignQualitative descriptive study conducted using content analysis to clarify the aspect of emotional labor of PHNs regarding interpersonal support.MethodsIndividual semi‐structured interviews were conducted with 28 public health nurses employed by seven city governments in Japan. The data items obtained were categorized using deductive content analysis.ResultsWhen attempting interpersonal support, PHNs showed the following aspects of emotional labor: (1) negative emotions towards residents and resident‐focused emotions; (2) emotional rules based on duty performance, emotional rules based on fairness, emotional rules based on intimacy; (3) adaptive emotional regulation, maladaptive emotional regulations and (4) emotional expressions based on friendliness, emotional expressions based on calmness.ConclusionPHNs used adaptive emotional regulation in some cases and sometimes resorted to maladaptive forms. A system of support should be established to ensure the smooth and effective provision of interpersonal support.
Central monitoring of depression and anxiety symptoms reduces placebo responses in depression clinical trials: A post hoc exploratory analysis of data from the phase III CCT‐004 trial of vortioxetine
Authors: Watanabe Yoshinori , Nishimura Akira , Kikuchi Toshiaki , Sawada Norifusa , Imazaki Manami , Inada Isao , Watanabe Koichiro 
Published: September 2022

AbstractAimClinical trials of antidepressants often fail to demonstrate their efficacy versus placebo, suggesting that patient selection based on physician ratings of depression may contribute to a high placebo response.MethodsIn the CCT‐004 trial of vortioxetine, central monitoring was employed to compare physician and patient ratings of depression and anxiety at baseline and over time to identify factors contributing to a large placebo response, as well as to explore the potential of a unique patient‐rated clinical measure combining QIDS‐J and Himorogi Self‐rating Anxiety Scale (HSAS), to contribute to optimal patient selection at baseline and patient monitoring over time.ResultsThe CCT‐004 trial showed similar trends between the QIDS‐J and MADRS (Montgomery‐Åsberg Depression Rating Scale) ratings. It was suggested that central monitoring of the QIDS‐J and MADRS ratings of depression and anxiety symptoms helped reduce the baseline score inflation by calling the study sites' attention to discrepancies between these ratings at baseline; it also allowed these ratings to be assessed for their concordance over time. Of note, MDD patients with baseline QIDS‐J scores ≥11/HSAS ≤19 were associated with the smallest placebo response, with the effect size being larger than that for those with QIDS‐J scores ≤10/HSAS ≥20.ConclusionThe use of both physician and patient ratings of depression and anxiety symptoms at baseline and over time, as well as their central monitoring, helped minimize the baseline score inflation and optimize patient monitoring over time, and allowed the antidepressant to be evaluated for its full therapeutic potential.
Excellent flame retardancy and air stability through surface coordination of few‐layer black phosphorus with TiL4 in epoxy resin
Authors: Diao Wenjie , Wang Kunxin , Jiao Enxiang , Yang Hui , Li Zhao , Wu Kun , Shi Jun 
Published: September 2022

AbstractBlack phosphorus (BP) has been attractive for many research groups as its promising properties. However, the poor air stability of BP has limited its practical applications. To simultaneously address this problem and improve the flame retardancy of BP in epoxy resin (EP), a surface coordination strategy was proposed. Herein, a titanium ligand (denoted as TiL4) was designed to coordinate BP nanosheets, which can occupy the lone pair electrons of BP. The Ti–P coordination contributed to the improvement of ambient stability of BP. The serious degradation was observed from pure BP owing to the oxidation. Whereas, the surface coordination can impede the ambient degradation rate of BP by 74.07%. With the addition of 1.5 wt% TiL4@BP, the char yield of EP nanocomposites was increased by 20.55% due to the catalytic charring effect of TiL4@BP. The incorporation of 1.5 wt% TiL4@BP can reduce the peak of heat release rate and total heat release values of EP by 29.41% and 23.32%. The EP/TiL4@BP 1.5 also can pass the UL‐94 V‐0 rating, and its value of limiting oxygen index was enhanced by 13.60%. The improvement in the flame retardancy of BP in EP can be largely ascribed to synergistic catalytic charring effects between BP and TiL4. The condense and compact char layer can act as a physical barrier to restrict the exchange of pyrolytic products and the transfer of heat. In addition, the free radical quenching effect of BP nanosheets also accounted for the excellent flame retardant performance of EP. This work proposed a reference for synchronically obtaining the improvement for the air stability and flame retardant performance of BP.