bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis, Cellular Uptake, and Photodynamic Activity of Oligogalactosyl Zinc(II) Phthalocyanines
Authors: Xiong Junlong , Xue Evelyn Y. , Ng Dennis K. P. 
Published: September 2022

AbstractA series of di‐α‐substituted zinc(II) phthalocyanines with different number of galactose moieties, ranging from 1 to 8, namely Pc‐galn (n=1, 2, 4, and 8) were designed and synthesized. The synthesis involved the copper‐catalyzed azide‐alkyne cycloaddition reaction of a mono‐ or dialkynyl zinc(II) phthalocyanine with an acetyl‐protected galactosyl azide or its dendritic derivative with four acetyl‐protected galactosyl groups, followed by removal of the acetyl protecting groups via alkaline hydrolysis. In N,N‐dimethylformamide, these oligogalactosyl phthalocyanines were non‐aggregated as shown by the strong Q‐band absorption and fluorescence emission. Owing to the di‐α‐substitution, they also behaved as efficient singlet oxygen generators upon light irradiation with a singlet oxygen quantum yield of 0.84. The spectroscopic and photophysical properties were not affected by the number of galactosyl units. In contrast, the compounds became significantly aggregated and quenched in phosphate‐buffered saline. Their cellular uptake was then studied using a range of cell lines, which generally followed the order Pc‐gal1>Pc‐gal2≈Pc‐gal4>Pc‐gal8. Interestingly, the di‐galactosyl analogue exhibited selective uptake against HeLa human cervical carcinoma cells through an energy‐dependent pathway instead of the expected asialoglycoprotein receptor. Upon light irradiation, it could effectively kill the cells with a half‐maximal inhibitory concentration of 0.58 μM.
Kinase‐Catalyzed Crosslinking and Immunoprecipitation (K‐CLIP) to Explore Kinase‐Substrate Pairs
Authors: Beltman Rachel J. , Pflum Mary Kay H. 
Published: September 2022

AbstractKinases are responsible for phosphorylation of proteins and are involved in many biological processes, including cell signaling. Identifying the kinases that phosphorylate specific phosphoproteins is critical to augment the current understanding of cellular events. Herein, we report a general protocol to study the kinases of a target substrate phosphoprotein using kinase‐catalyzed crosslinking and immunoprecipitation (K‐CLIP). K‐CLIP uses a photocrosslinking γ‐phosphoryl‐modified ATP analog, such as ATP‐arylazide, to covalently crosslink substrates to kinases with UV irradiation. Crosslinked kinase‐substrate complexes can then be enriched by immunoprecipitating the target substrate phosphoprotein, with bound kinase(s) identified using Western blot or mass spectrometry analysis. K‐CLIP is an adaptable chemical tool to investigate and discover kinase‐substrate pairs, which will promote characterization of complex phosphorylation‐mediated cell biology. © 2022 Wiley Periodicals LLC.Basic Protocol 1: Kinase‐catalyzed crosslinking of lysatesBasic Protocol 2: Kinase‐catalyzed crosslinking and immunoprecipitation (K‐CLIP)
Screening for eating disorders across genders in college students: Initial validation of the brief assessment of stress and eating
Authors: Forbush Kelsie T. , Richson Brianne N. , Swanson Trevor J. , Thomeczek Marianna L. , Negi Sonakshi , Johnson Sarah N. , Chapa Danielle A. N. , Morgan R. William , O'Brien Colin J. , Gould Sara R. , Christensen Kara Alise , Chen Yiyang 
Published: September 2022

AbstractGiven that eating disorders (EDs) are relatively common in college populations, it is important to have reliable and valid tools to identify students so that they can be referred to evidence‐based care. Although research supports the psychometric properties of existing ED screens for identifying cases of EDs, most studies have been conducted in samples of young white‐majority women or have not reported the psychometric properties of the screening tool in men.ObjectiveThe purpose of the current study was to validate a brief, 10‐item screening tool for the identification of EDs—the brief assessment of stress and eating (BASE).MethodParticipants were college students (N = 596; 68.2% cisgender women) from a large Midwestern university who completed the BASE and SCOFF. The Eating Disorders Diagnostic Survey was used to generate DSM‐5 ED diagnoses. We evaluated area under the curve (AUC) for both receiver operating curves (ROC) and precision‐recall curves (PRC).ResultsBoth the BASE and SCOFF performed significantly better than chance at identifying probable EDs in cisgender women (BASE AUC: ROC = .787, PRC = .633, sensitivity = .733, specificity = .697; SCOFF AUC: ROC = .810, PRC = .684, sensitivity = .793, specificity = .701). However, the BASE (AUC: ROC = .821, PRC = .605, sensitivity = .966, specificity = .495) significantly outperformed the SCOFF (AUC: ROC = .710, PRC = .354, sensitivity = .828, specificity = .514) for identifying probable EDs in cisgender college men.DiscussionThe BASE is appropriate for student healthcare and college research settings. Because the BASE outperforms the SCOFF in college men, results from the current study are expected to contribute to improved identification of EDs on college campuses.Public SignificanceThe BASE is a new screening tool to identify eating disorders. The BASE performed as well as, if not better than, the SCOFF (particularly in men). Given the need for brief, psychometrically strong, and unbiased ED screening tools in college students, the current study helps address an unmet student healthcare need that we expect will contribute to improved identification of EDs on college campuses.
Impacts of insect frass and cadavers on soil surface litter decomposition along a tropical forest temperature gradient
Authors: Hwang Bernice C. , Giardina Christian P. , Litton Creighton M. , Francisco Kainana S. , Pacheco Cody , Thomas Naneaikealaula , Uehara Tyler , Metcalfe Daniel B. 
Published: September 2022

AbstractInsect herbivores play important roles in shaping many ecosystem processes, but how climate change will alter the effects of insect herbivory are poorly understood. To address this knowledge gap, we quantified for the first time how insect frass and cadavers affected leaf litter decomposition rates and nutrient release along a highly constrained 4.3°C mean annual temperature (MAT) gradient in a Hawaiian montane tropical wet forest. We constructed litterbags of standardized locally sourced leaf litter, with some amended with insect frass + cadavers to produce treatments designed to simulate ambient (Control = no amendment), moderate (Amended‐Low = 2 × Control level), or severe (Amended‐High = 11 × Control level) insect outbreak events. Multiple sets of these litterbags were deployed across the MAT gradient, with individual litterbags collected periodically over one year to assess how rising MAT altered the effects of insect deposits on litter decomposition rates and nitrogen (N) release. Increased MAT and insect inputs additively increased litter decomposition rates and N immobilization rates, with effects being stronger for Amended‐High litterbags. However, the apparent temperature sensitivity (Q10) of litter decomposition was not clearly affected by amendments. The effects of adding insect deposits in this study operated differently than the slower litter decomposition and greater N mobilization rates often observed in experiments which use chemical fertilizers (e.g., urea, ammonium nitrate). Further research is required to understand mechanistic differences between amendment types. Potential increases in outbreak‐related herbivore deposits coupled with climate warming will accelerate litter decomposition and nutrient cycling rates with short‐term consequences for nutrient cycling and carbon storage in tropical montane wet forests.
Feeding ecology of Ellochelon vaigiensis (Quoy & Gaimard, 1825) living in the Mekong Delta, Vietnam
Authors: Dinh Quang Minh , Truong Ngon Trong , Nguyen Ton Huu Duc , Lam Tran Thi Huyen , Nguyen Tien Thi Kieu , Le Dung Quang , Das Simon Kumar 
Published: September 2022

AbstractEllochelon vaigiensis (squaretail mullet) adapts to a wide salt spectrum, grows quickly and is easy to raise with other species, so it is the object of attention in aquaculture. Information on the biology and ecology of the species, diet, in particular, is still scattered. Here, we explore the feeding habit, feeding intensity, and food composition of the squaretail mullet. A total of 942 fish (526 males and 416 females) were collected from November 2020 to October 2021 at four coastal sites in the Mekong Delta, Vietnam. The squaretail mullet is an algi‐omnivorous fish, as their relative gut length (1.81) falls into the range 1–3, and the diet is mostly based on algae. The feeding intensity is high due to the high value of the fullness index (4.39 ± 0.08%). The fullness index did not vary by sampling site and month, while RGL and FI changed depending on sex. Bacillariophyta (49.13%), detritus derived from organic matter (30.37%), and Cyanophyta (18.39%) are the dominant food items in the diet composition of E. vaigiensis, in which detritus is the most important food with the highest IRI index. Besides, Euglenophyta (1.00%), Chlorophyta (0.95%), Paramecium (0.06%), Copepoda (0.04%), Rotatoria (0.03%), Polychaeta (0.02%), and Cladocera (0.01%) are also recorded and ranked based on their biovolume. Some differences in diet composition are observed between immature and mature at different seasons and their interactions. Our results increase the knowledge about the feeding ecology of squaretail mullet and can help the sustainable management of this commercially important fish species.
A simulation study of an innovation‐driven sustainable development model and its impacts and applications: A case study of the Yangtze River Economic Belt
Authors: Wang Wei , Wang Haofei 
Published: September 2022

AbstractThe economic development process is accompanied by energy consumption and environmental pollution, difficulties which must be overcome to realize sustainable development. A system dynamics model is constructed to simulate the pattern characteristics of an innovation‐driven coupling system scenario for economic development, energy consumption and environmental pollution in the Yangtze River Economic Belt. Then, the impulse response relationship between technological progress and economic, energy and environmental system indexes is further explored by using a vector autoregression (VAR) model. The results show that (1) the model is suitable for predictive analysis. (2) Adjusting the model parameters shows that the innovation‐driven development mode is the optimal sustainable development mode of the Yangtze River Economic Belt. (3) VAR model analysis shows that technological progress has a significant long‐term positive impact on the economic development system index, and the growth of primary industry is the most significant. Technological progress has a significant long‐term inhibition effect on energy consumption and environmental pollution system indicators, among which the inhibition effect on wastewater discharge and power consumption is the strongest. Finally, suggestions are proposed to increase investment in agricultural technology, smart countryside, and wastewater treatment.
DNA damage accumulation and repair defects in FLT3‐ITD acute myeloid leukemia: Implications for clonal evolution and disease progression
Authors: Lagunas‐Rangel Francisco Alejandro 
Published: September 2022

AbstractAcute myeloid leukemia is a group of hematological diseases that have a high mortality rate. During the development of this pathology, hematopoietic cells acquire chromosomal rearrangements and multiple genetic mutations, including FLT3‐ITD. FLT3‐ITD is a marker associated with a poor clinical prognosis and involves the activation of pathways such as PI3K/AKT, MAPK/ERK, and JAK/STAT that favor the survival and proliferation of leukemic cells. In addition, FLT3‐ITD leads to overproduction of reactive oxygen species and defective DNA damage repair, both implicated in the appearance of new mutations and leukemic clones. Thus, the purpose of this review is to illustrate the molecular mechanisms through which FLT3‐ITD generates genetic instability and how it facilitates clonal evolution with the generation of more resistant and aggressive cells. Likewise, this article discusses the feasibility of combined therapies with FLT3 inhibitors and inhibitors of DNA repair pathways.
The impact of policy design on willingness to pay for ecosystem services from prairie strips
Authors: Khanal Badri , Schoengold Karina , Mieno Taro , Schulte Moore Lisa 
Published: September 2022

AbstractPrairie strips on agricultural lands are supported by the Conservation Reserve Program and provide environmental benefits such as reduced soil loss and improved wildlife habitat. The current study measures the value that the public places on those benefits and if that value changes under different policy designs. The policy design varies by who runs the program (state agency vs. nongovernment organization) and who has enrollment priority (historically managed land vs. degraded land). Results from a choice experiment indicate significant overall public support for the expansion and that willingness to pay is highest with priority for land historically managed in conservation‐oriented way.
A 2-Year Randomized Controlled Trial With Low-Dose B-Vitamin Supplementation Shows Benefits on Bone Mineral Density in Adults With Lower B12 Status
Authors: Clements Michelle , Heffernan Maria , Ward Mary , Hoey Leane , Doherty Leanne C , Hack Mendes Roberta , Clarke Michelle M , Hughes Catherine F , Love Ingrid , Murphy Shauna , McDermott Eilish , Grehan Jennifer , McCann Adrian , McAnena Liadhan B , Strain JJ , Brennan Lorraine , McNulty Helene 
Published: September 2022

ABSTRACT
Folate, vitamins B12, B6, and riboflavin are required for one-carbon metabolism and may affect bone health, but no previous randomized trial has investigated all four nutrients in this context. We investigated the effect of low-dose B-vitamins for 2 years on bone mineral density (BMD) in a dual-centered, 2-year randomized controlled trial (RCT) in adults aged ≥50 years. Eligible participants not consuming B-vitamin supplements or fortified foods &gt;4 times weekly were randomized to receive daily either combined folic acid (200 μg), vitamin B12 (10 μg), vitamin B6 (10 mg), and riboflavin (5 mg), or “active” placebo, whereby both the intervention and placebo groups received vitamin D (10 μg). BMD was assessed before and after intervention using dual-energy X-ray absorptiometry (DXA) scanning of the total hip, femoral neck, and lumbar spine (L1 to L4). Of 205 eligible participants randomized, 167 completed the trial in full. B-vitamin intervention resulted in increases in serum folate (p &lt; 0.001), serum B12 (p &lt; 0.001), and plasma pyridoxal-5-phosphate (p &lt; 0.001) and decreases in functional biomarkers of B-vitamin status, erythrocyte glutathione reductase activation coefficient (p &lt; 0.001), serum methylmalonic acid (MMA; p &lt; 0.001), and serum total homocysteine (p &lt; 0.001). B-vitamin intervention had no overall effect on BMD, which declined in both treatment groups by approximately 1% (ranging from −0.7% to −1.4%). However, in participants with lower baseline B12 status (serum B12 &lt;246 pmol/L or MMA ≥0.22 μmol/L), B-vitamin intervention reduced the 2-year BMD decline versus placebo: adjusted mean (95% confidence interval [CI]) change of −0.003 (−0.008, 0.002) versus −0.015 (−0.021, −0.010) g/cm2 at the total hip and −0.004 (−0.010, 0.001) versus −0.013 (−0.018, −0.007) g/cm2 at the femoral neck. In conclusion, the findings indicate that although low-dose B-vitamin intervention for 2 years had no overall effect on BMD, improving B-vitamin status appears to have specific benefits for bone health in adults with lower B12 status. © 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
Orofacial abnormalities in mucopolysaccharidosis and mucolipidosis typeIIandIII: A systematic review
Authors: de Bode Chiel J. , Dogterom Emma J. , Rozeboom Antoinette V. J. , Langendonk Janneke J. , Wolvius Eppo B. , van der Ploeg Ans T. , Oussoren Esmée , Wagenmakers Margreet A. E. M. 
Published: September 2022

AbstractMucopolysaccharidoses (MPSs) and mucolipidosis II and III (ML II and III) often manifest with orofacial (progressive) abnormalities, which may have a major impact on quality of life. However, because these patients have multiple somatic health issues, orofacial problems are easily overlooked in clinical practice and available literature on this topic solely consists of case reports, small case series, and small cohort studies. The aim of this systematic review was to gain more insight in the nature and extent of orofacial abnormalities in MPS, ML II, and III. A systematic review of all previously published articles addressing orofacial abnormalities in MPS, ML II, and III was performed. Both clinical studies and case reports were included. Outcome was the described orofacial abnormalities, subdivided into abnormalities of the face, maxilla, mandible, soft tissues, teeth, and occlusion. The search resulted in 57 articles, describing orofacial features in 340 patients. Orofacial abnormalities were present in all subtypes of MPS, ML II, and III, and consisted of thickened lips, a hypoplastic midface, a high‐arched palate, hypoplastic condyles, coronoid hyperplasia, macroglossia, gingival hyperplasia, thick dental follicles, dentigerous cysts, misshapen teeth, enamel defects, and open bite. Orofacial abnormalities are present in all subtypes of MPS, ML II, and III. As orofacial abnormalities may cause complaints, evaluation of orofacial health should be part of routine clinical care.
Influence of interspecific competition on mule deer birthing and rearing site selection
Authors: Sallee Daniel W. , McMillan Brock R. , Hersey Kent R. , Petersen Steven L. , Larsen Randy T. 
Published: September 2022

AbstractUngulates often alter behavior and space use in response to interspecific competition. Despite observable changes in behavior caused by competitive interactions, research describing the effects of competition on survival or growth is lacking. We used spatial modeling to determine if habitat use by female mule deer (Odocoileus hemionus) was affected by other ungulate species prior to, during, and after parturition. We conducted our study in the Book Cliffs region of eastern Utah, USA, during 2019 and 2020. We used resource selection function (RSF) analysis to model space use of 4 ungulate species that potentially competed with mule deer: bison (Bos bison), cattle, elk (Cervus canadensis), and feral horses. We incorporated RSF models for competing species into a random forest analysis to determine if space use by mule deer was influenced by these other ungulate species. We used survival and growth data from neonate mule deer to directly assess potential negative effects of other ungulates. Habitat use by elk was an important variable in predicting use locations of mule deer during birthing and rearing. The relationship was positive, suggesting interference competition was not occurring. Survival of neonate mule deer increased as the probability of use by elk increased (hazard ratio = 0.185 ± 0.497 [SE]). Further, probability of use by elk in rearing habitat had no influence on growth of neonate mule deer from birth to 6 months of age, suggesting that exploitative competition was not occurring.
Self‐Healing, High‐Strength, and Antimicrobial Polysiloxane Based on Amino Acid Hydrogen Bond
Authors: Guo Ruilu , Zhou Lixia , Lin Jiwei , Chen Guangxin , Zhou Zheng , Li Qifang 
Published: September 2022

AbstractRecent years have witnessed the rapid development of self‐healing and recyclable materials because they can extend the life of the material. For polysiloxane materials, exploring polysiloxanes with high‐strength and self‐healing properties remains a challenge. In this work, a high‐strength and self‐healing polysiloxane containing N‐acetyl‐L‐cysteine (NACL) side groups is prepared. The NACL is used to form strong hydrogen bonds to build a self‐healing network. Molecular simulations help explain the reasons and processes for the repair of modified polysiloxanes. On the one hand, the obtained modified polysiloxanes have good self‐healing properties. The self‐healing efficiency of modified polysiloxane can reach 96.9%. As the number of NACL increases, the tensile strength of the modified polysiloxane increases. For PMVS‐30%NACL, the tensile strength can reach 4.36 MPa, and the strain can reach 586%. On the other hand, modified polysiloxane has an apparent inhibitory effect on Staphylococcus aureus. With the increase in the number of NACL, the antibacterial effect of modified polysiloxane is more obvious. Furthermore, NACL is a bio‐based amino acid with excellent biocompatibility. This work expands the idea of designing and synthesizing high‐strength polysiloxanes with antibacterial properties. It has great potential in the field of polysiloxane antimicrobial coatings.
Chinese expert consensus on diagnosis and treatment of radiation pneumonitis
Authors: Zhou Caicun , Yu Jinming 
Published: September 2022

AbstractRadiotherapy is one of the main tumor treatment modalities that provides great survival benefits to patients with cancer. However, it is important to pay attention to the occurrence of adverse events after radiotherapy and take early measures. Radiation pneumonitis (RP) is a major adverse response in patients with cancer receiving thoracic radiotherapy, and it affects the quality of life and survival of patients. We analyzed the latest evidence on RP, and summarized research developments on the pathophysiological mechanism, risk factors, clinical manifestations, diagnosis and differential diagnosis, classification, treatment, and prognosis to form a consensus. With the development of comprehensive treatments, including immunotherapy, the diagnosis and identification of RP has become more complex, requiring multidisciplinary discussion and decision‐making. In the future, it is critical to explore more effective treatments, various risk factors, and precautions for RP.
Synthesis of One‐Dimensional Mo2C‐Embedded Carbon Nanofibers with Enhanced Lithium Storage Capacity for Lithium‐Ion Batteries
Authors: Ragupathi Jeevani , Annal Therese Helen 
Published: September 2022

AbstractMXenes are in the limelight nowadays due to their fascinating structure and outstanding physical and chemical properties. Mo2C, a part of MXenes, has a unique structure where the carbon present in the interstitial voids between the layers of the molybdenum (Mo) atom is very sensitive to even a small addition of electrons. Herein, we have pursued the synthesis of one‐dimensional Mo2C‐embedded carbon nanofibers (Mo2C@CNF) as a potential anode material for lithium‐ion batteries. The Mo2C@CNF is synthesized by simple electrospinning (E‐Spin) technique followed by iodization and thermal treatment at 1000 °C under Argon. The iodization process plays a major role in the formation of Mo2C@CNF, which creates a stronger backbone and high yield of carbon content during the carbonization process. The presence of highly disordered carbon in Mo2C@CNF was demonstrated by FESEM, XRD, RAMAN, and HRTEM studies. The electrochemical performance studies of Mo2C@CNF, carried out using 2032 type coin cell configuration, show an enhanced specific capacity of 1492 mAh g−1 at 0.15 C‐rate during the first discharge. These high‐capacity values obtained due to the synergistic interaction between the embedded Mo2C and carbon nanofibers open up a new avenue to high performance carbide based anodes for energy storage applications.