bluuebunny/crossref_metadata_2025_split huggingface dataset:
The mammalian Kalrn locus gives rise to several novel long non-coding RNAs
Authors: Pal Muneesh , Chaubey Divya , Tanwar Mohit , Pillai Beena 
Published: February 2022

Abstract
The KALRN gene encodes several multi-domain protein isoforms that localise to neuronal synapses, and play dynamic roles in shaping axonal outgrowth, dendrite morphology and dendritic spine re-modelling. The genomic locus is implicated in several neurodevelopmental and neuropsychiatric diseases including autism, schizophrenia and bipolar disease. Mutations in the coding regions, inherited in a classical Mendelian manner, have also been implicated in certain forms of autism and intellectual disability. At the molecular level, the protein isoforms, encoded by reported transcript isoforms, share some core domains arising from the central exons, while other domains, especially towards the C terminal may be selectively incorporated. This heterogeneity seems to confer the ability to grow and retract dendritic spines, thus making Kalirin a critical and dynamic player in dendritogenesis. We have previously shown that in the zebrafish genome, a novel brain specific non-coding RNA arising from the 5’ end of the Kalirin gene, durga regulates neuronal morphology. In search of the mammalian equivalent, we characterized the mammalian KALRN loci in detail, annotating multiple novel non-coding RNAs, including linear and circular variants, through analysis of transcriptomics data and experimental approaches. By comparing the mouse and human loci and studying the expression of the novel lncRNAs arising from the locus during differentiation of primary cortical neurons in culture, we show that certain non-coding RNAs arising from the locus show a temporal expression profile that coincides with a subset of Kalirin protein coding isoforms. In humans, mouse and zebrafish the 5’end of the KALRN locus gives rise to a chromatin associated lncRNA that is present in adult ovaries besides being expressed during brain development and in certain regions of the adult brain. Besides correcting some of the annotations available in public databases, we propose that this lncRNA arising from the 5’end of the KALRN locus is the mammalian ortholog of zebrafish lncRNA durga.
Assessment of Irrigation Potential in Jewuha Watershed, Middle Awash River Basin, Ethiopia
Authors: Dinku Manamno Beza , Kebede Habtamu Hailu 
Published: February 2022

Abstract
Ethiopia is endowed with immense water and land resources that could be tapped and used for irrigation development. However, little has been done so far due mainly to lack of comprehensive knowledge on the potential of these resources. This study was, therefore, taken up to assess the irrigation potential in data scarce Jewuha watershed of the Awash River Basin. To achieve the objective of the study, the suitability of the land for both surface and pressurized irrigations was estimated using a parametric evaluation technique combined with the FAO land evaluation framework in the GIS environment with multi-criteria analysis of irrigation suitability factors. The irrigation water demands of major crops adopted in the area were determined using CROPWAT software. The surface water potential at the sub watershed was estimated using a combination of SWAT model and the spatial proximity regionalization technique. The performance of the SWAT model was checked by statistical parameters. The irrigation suitability analysis reveals that 51372ha of the study area are suitable and 16274ha are unsuitable for surface irrigation system. Furthermore, 52768ha of the area are suitable and 15088ha are unsuitable for a sprinkler irrigation system. For drip irrigation, 52751ha are suitable and 14855ha are unsuitable. The calibration and validation of SWAT model showed that the model has performed well to simulate the hydrology of the watershed with a coefficient of determination (R2), Nash-Sutcliffe efficiency (NSE) and Percent of bias (PBIAS) of 0.74, 0.73 and 0.80 for calibration and 0.71, 0.70 and 7.90 for validation, respectively. From a combined analysis of the available land and water resources, the gross irrigation potential of the area is estimated to be 12,997ha, of which 3098ha of land is exclusively suitable for surface irrigation and 9,899ha is suitable for pressurized irrigation systems.
TiO2 Nanosheet Supported MnCeOx: A Remarkable Catalyst with Enhanced Low-Temperature Catalytic Activity in o-DCB Oxidation
Authors: He Xu , Guo Haiwei , Liu Xiaoyao , Wen Jiaxin , Ren Gengbo , Ma Xiaodong 
Published: February 2022

Abstract
Morphology engineering was an effective strategy for 1,2-dichlorobenzene (o-DCB) oxidation. Herein, TiO2 nanosheet supported MnCeOx (TiMn15Ce30-NS) showed excellent catalytic activity with T50% = 156 oC and T90% = 238 oC, which was better than the T50% = 213 oC and T90% = 247 oC for TiO2 nano truncated octahedron supported MnCeOx (TiMn15Ce30-NTO). TiMn15Ce30-NS also exhibited enhanced water resistance (T50% = 179 oC, T90% = 240 oC), and good stability with the o-DCB conversion retained at 98.9% for 12 h at 350 oC. The excellent catalytic activity of TiMn15Ce30-NS could be mainly ascribed to the preferentially exposed {001} crystal plane which favored the higher concent of Mn4+ along with surface active oxygen, as well as stronger interaction between MnOx and CeOx in TiMn15Ce30-NS. The present results deepen the understanding of the morphology-dependent effect on o-DCB oxidation.
Closed Reduction and Percutaneous Pinning Versus Open Reduction and Internal Fixation for Displaced Rockwood and Wilkins’ Type C Thumb Metacarpal Base Fractures in Children
Authors: Qiao Fei , Cheng Lei , Jiang Fei 
Published: February 2022

Abstract
Background
This retrospective study compared closed reduction and percutaneous pinning (CRPP) versus open reduction internal fixation (ORIF) methods in the treatment of severely displaced Rockwood and Wilkins’ type C (RWC) thumb metacarpal base fractures in children.
Methods
The article included 30 patients (14 boys, 16 girls; mean age 10.3 years; age range 6.2 to 14.0 years) with severely displaced RWC fractures. All patients were treated by CRPP and ORIF. The clinical results were assessed according to the criteria of modified Mayo scores. The mean follow-up period was 30.3 months (range 24.0–45.0 months). The level of significance was p <0.05.
Results
All fractures healed within 7 weeks postsurgery, regardless of the surgery method used, and differences between groups were observed in time to union. Recovery time after CRPP treatment averaged 4.2 weeks and was faster than that after ORIF, which averaged 4.7 weeks (P<0.05). The operative time for CRPP averaged 20 minutes, which was faster than that of ORIF (P<0.05). The overall minor complication rates were 6.3% for patients in the CRPP group and 21.4% for patients in the ORIF group (P>0.05). No major complications were observed in patients in the two groups. The clinical outcomes of CRPP were evaluated by modified Mayo scores: 15 patients had excellent outcomes, and one patient had a good outcome in the CRPP group. Twelve patients had excellent outcomes, and 2 patients had a good outcome in the OPIF group. (P>0.05). There were no refractures or incidences of nonunion, avascular necrosis (AVN), osteomyelitis, or premature physeal closure.
Conclusion
The CRPP and ORIF data of considerably displaced RWC fractures in children revealed approximately equal results for patient outcomes and rates of complications. We assert that CRPP is superior to ORIF as a treatment for RWC fractures because CRPP minimizes the operative time, precludes the need for an incision and enables faster fracture union; thus, it is our preferred treatment method.
TBK1 Participates in Glutaminolysis by Mediating the Phosphorylation of RIPK3 to Promote Endotoxin Tolerance
Authors: Pan Lehan , Yang Lian , Yi Zhujun , Zhang Wenfeng , Gong Jianping 
Published: February 2022

Abstract

Background: TBK1 (TRAF-associated NF-κB activator (TANK)-binding kinase 1), a nonclassical IκB kinase (IKK), and its effect on inflammation have not been entirely clarified. This study aimed to determine how TBK1 participates in the catabolism of glutamine by mediating the phosphorylation of receptor-interacting protein kinase 3 (RIPK3) and promoting macrophage endotoxin tolerance (ET). Methods: Lipopolysaccharide (LPS)-induced ET mice models were used for in vivo studies. Hematoxylin and eosin staining, immunofluorescence staining and immunohistochemical staining protocols were used to analyze histological changes in the liver, and the related key pathway proteins were analyzed by western blotting. Kupffer cells (KCs) and bone marrow-derived macrophages (BMDMs) isolated from C57BL/6 mice were used to establish ET models. ELISA was used to detect the levels of necrosis factor-α (TNF-α), interleukin-10 (IL-10) and α-ketoglutarate (α-KG). The differentiation of M1/M2 macrophages was assessed by flow cytometry for assessing the cell-surface markers, CD86 and F4/80. The related key proteins were analyzed by western blotting and immunofluorescence staining. Results: The phosphorylation of TBK1 was higher in the ET group than in the NET group in vitro and in vivo. As TBK1 was overexpressedd by shRNA, the phosphorylation level of TBK1 was increased, and the protective effect of ET was also strengthened. The TBK1 protein directly interacts with the RIPK3 protein and mediates the phosphorylation of RIPK3 in macrophages. According to our co-IP results, the glutamate dehydrogenase 1 (GLUD1) and RIPK3 proteins interact in ET models. In addition, activated RIPK3 can directly bind to GLUD1, thereby improving its catalytic activity and increasing the production of α-ketoglutarate (α-KG) in macrophages. α-KG generated from glutaminolysis promotes M2 activation and restricts M1 polarization to induce the macrophage tolerance of LPS. These findings reveal a novel mechanism for the metabolic control of inflammation and for the induction of ET by modulating glutamine metabolism. Conclusion: As a result of TBK1 regulating the phosphorylation level of RIPK3, overexpressed TBK1 could enhance the tolerance of macrophages to endotoxin through glutaminolysis, which unveils the potential for therapeutic targeting of the metabolic status of macrophages.
MiR-362-5p targets CDK2 and inhibits tumorigenesis in renal cell carcinoma
Authors: Liu Yang , Tang Ja , Yang Dandan 
Published: February 2022

Abstract
Background: Renal cell carcinoma (RCC) is a very common malignant tumor. Identifying novel biomarkers is essential to predict the progression and prognosis of RCC. MicroRNA (miRNA) expression profiles have been extensively studied in major cancers. The purpose of this study was to evaluate the expression of miR-362-5p in clear cell renal cell carcinoma (ccRCC) and to evaluate its potential mechanisms in RCC.Material and Methods:In this study, 4 groups of clear ccRCC and adjacent miRNA expression profiling chips were screened using the GEO database. Six miRNAs in 4 sets of expression spectrum chips were expressed at low levels in ccRCC tissues, among which miR-362-5p exhibited the greatest downregulation. The effects of miR-362-5p were observed by real-time fluorescence quantitative PCR, cell transfection experiments, CCK-8 experiments and flow cytometry cell cycle assays. Target genes of miR-362-5p were verified by luciferase reporter assay and confirmed by western blot.Results:Through data analysis, miR-362-5p was selected for study. Cell growth curves and flow cytometry detection showed that overexpression of miR-362-5p inhibited the growth of RCC cells and induced cell cycle arrest at the G0/G1 phase. Bioinformatics analysis indicated that cyclin-dependent kinase 2 (CDK2) could be a potential target of miR-362-5p. Mechanistic studies revealed that miR-362-5p is directly responsible for inhibiting CDK2 expression in ccRCC cell lines.Conclusion:In summary, our study confirmed that miR-362-5p in ccRCC tissues is significantly downregulated. MiR-362-5p regulates CDK2 to inhibit the proliferation of clear cell renal cell carcinoma. CDK2 regulates tumor cell growth and cell cycle progression, which arrests the cell cycle at the G1/S phase.
High-throughput Sequencing Reveals the Dominance of Shewanella Species in the Intestinal Microbiota of Barbour’s Seahorses (Hippocampus Barbouri)
Authors: Ortega Rose Chinly Mae Huerte , Tabugo Sharon Rose , Martinez Joey Genevieve , Balcázar José 
Published: February 2022

Abstract
In this study, taxonomic classification of intestinal microbiota from healthy Barbour’s seahorses (Hippocampus barbouri) was determined as it plays an important role in host nutrition and immunity. Genomic DNA was extracted from the intestinal mucus samples of eleven Barbour’s seahorses, which were subjected to high-throughput sequencing of bacterial 16S rRNA genes targeting V1-V3 regions. The results revealed that the most abundant operational taxonomic units (OTUs) were affiliated to the genus Shewanella. Our findings may provide baseline data for further studies in order to explore the potential implications of intestinal microbiota to seahorses in terms of health status, development, growth, and survival.
Quantitative estimation of essential/toxic elemental levels in the serum of esophagus cancer patients in relation to controls
Authors: Qayyum Muhammad Abdul , Sultan Muhammad Habib , Farooq Zahid , Muddassir Khawaja , Farooq Tahir , Irfan Ahmad 
Published: February 2022

Abstract
Esophageal cancer is a very deadly disease ranking 8th most common cancer in terms of incidence and the 6th highest in terms of mortality both in USA and around the world. A growing body of evidence indicated that changes in the concentrations of essential and toxic elements may affect/increase esophagus carcinoma risk. The aim of this study was to measure serum levels of essential and toxic (Fe, Na, Ca, K, Zn, Mg, Co, Se, Cu, Ni, Mn, Sr, Pb, Li, Sb, Cr, Ag, Cd, As and Hg) elements in patients with esophagus carcinoma and controls. Atomic absorption spectroscopy was used to determine serum concentrations of essential and toxic elements by using nitric acid/perchloric acid–based wet digestion method. Mean levels of Cu, Ni, Cr, Cd, Pb, As and Ag were exhibited to be significantly higher and mean Se, Co, Zn, Ca, Fe, Hg, Li and Mg were noted lower in the serum of cancer patients than controls. The correlation coefficients among the elements in the cancerous patients revealed significantly dissimilar communal relationships than the controls. Further, multivariate methods demonstrated considerably different apportionment between the elements in the cancerous patients and the controls. Significant inequalities in the elemental concentrations were also observed for esophagus cancer types (adenocarcinoma and squamous cell carcinoma) and stages (I, II, III, and IV) between the patients. Majority of the elements exposed perceptible disparities in their levels based on smoking habits, dietary habits, habitat and gender of the esophagus cancer patients and controls. Multivariate analysis of the essential and toxic elemental data explained significantly divergent apportionment in the serum of esophagus cancer patients when compared to controls.
Impact of Income Inequality on Health and Education in Africa: The Long run Role of Public Spending with Short Run Dynamics
Authors: Chatterjee Tonmoy , Teame Ghirmai Tesfamariam 
Published: February 2022

Abstract

In this paper we empirically investigated the long-run behavior of income inequality on the pattern of major social sector elements like health and education in African continent. In this context we have used a data set for 31 African countries for the period of 1980-2017. Our empirical model amalgamated with the rationality of theoretical underpinning claims that income inequality adversely affects health and education both in Short-run and long-run. However, it also claims that in the presence of efficient state intervention in the form of investment in both health and education unambiguously can better-off health and education scenarios in long-run, although the curse of income disparity remains with uphold in short-run. Apart from this, the present study also finds the threshold levels of government investments are required to make the process of better-off more sustainable for both health and education in the presence of income inequality in Africa. These results could be important for policymaking to boost health, education in Africa under the cloud of income disparity.
Remediation potential of a soil bacterium, Planomicrobium flavidum (S5-TSA-19) for 2,4,6-trinitrotoluene (TNT) i.e., a nitro-aromatic explosive in aquatic medium.
Authors: Lamba Jyoti , Anand Shalini , Dutta Jayanti , Rai Pramod Kumar 
Published: February 2022

Abstract
2,4,6-Trinitrotoluene (TNT), a nitro-aromatic explosive commonly used for defence and several non-violent applications is contributing to serious environmental pollution problems including human health. The current study investigated the remediation potential of a native soil isolate i.e., Planomicrobium flavidum (strain S5-TSA-19) against TNT. At a TNT concentration of 120 mg/L with a specified environment, the isolate remained incubated for 30 days in a minimal salt medium (MSM). Regular growth of the isolate, the concentration of TNT, nitrate, nitrite and ammonium ion were evaluated at a particular time during the experiment. Within 168 hours (i.e., 7 days) of incubation, P. flavidum co-metabolically degraded 100% TNT. The biodegradation procedure succeeded the first order kinetics mechanism. Formation of additional metabolites like 2,4-dinitrotoluene (DNT), 2,4-diamino-6-nitrotoluene (2-DANT) and 2-amino-4,6-dinitrotoluene (2-ADNT), were also witnessed. TNT seems to be non-toxic for the isolate, as it reproduced magnificently in explosive presence. For TNT degradation, the nitro-reductase enzyme was observed to be responsible. This is so far the first reporting of Planomicrobium flavidum, efficiently bioremediating TNT i.e., a nitro-aromatic compound via different degradation pathways, leading to the production of simpler as well as less harmful end products. Further, in the near future, at the field scale application Planomicrobium flavidum may be explored for the bioremediation of TNT (i.e., a nitroaromatic- compound) contaminated effluents.
An online human blood transcript correlation browser for easy access to the blood cell interactome
Authors: Gautvik Kaare M. , Sachse Daniel , Hinton Alexandra C. , Olstad Ole K. , Kiel Douglas P. , Hsu Yi-Hsiang , Utheim Tor P. , Lary Christine W. , Reppe Sjur 
Published: February 2022

Abstract
Background
Physical molecular interactions are the basis of intracellular signalling and gene regulatory networks, and comprehensive, accessible databases are needed for their discovery. Highly correlated transcripts may reflect important functional associations, but identification of such associations from primary data are cumbersome. Therefore, we have constructed a user-friendly online browser supporting identification of putative macromolecular interactions in human peripheral blood based on significant correlations at the transcriptional level.
Methods
The blood transcriptome was characterized by quantification of 17,328 RNA species, including ~341 mature microRNAs in 105 clinically well-characterized postmenopausal women. Intercorrelation of detected transcripts signal levels generated a matrix with >150 million correlations recognizing the human blood RNA interactome. The correlations with calculated adjusted p-values were made easily accessible by a freely available online browser.
Results
We document that significant transcript correlations within the giant matrix reflect experimentally documented interactions between select ubiquitous blood relevant transcription factors CREB1, GATA1, and the glucocorticoid receptor (GR, NR3C1). Their responsive genes recapitulated up to 91% of these as significant correlations, and were replicated in a separate cohort of 1204 individual blood samples from the Framingham Heart Study. Furthermore, experimentally documented mRNAs/miRNA associations were also reproduced in the matrix, and their predicted functional co-expression described. The blood transcript correlation browser is freely available at http://app.uio.no/med/klinmed/correlation-browser/blood/index.php and works on all commonly used internet browsers.
Conclusions
The matrix and web browser ought to be a valuable tool for easy access to the human blood transcriptome and molecular interactions documented as significant correlations at the RNA-level. This application should also be useful as a hypothesis generating tool for identification of regulatory mechanisms.
A Control Strategy for Improving the Accuracy of Lateral Tracking of Autonomous Vehicles
Authors: Liu Ping , Liu Zibin , Wu Chaohui , Wan Zhisong , Ding Weiping , Huang Haibo 
Published: February 2022

Abstract
Lateral tracking is one of the key technologies for autonomous vehicles. It is particularly important when vehicles are traveling at low speeds in narrow and complex scenes, such as parking lots and alleys. In order to improve the effective and accurate control of the traditional Pure Pursuit algorithm (PP) in the lateral tracking of low-speed autonomous vehicles, a continuous adaptive piecewise fitting method based on cubic Bezier curve was proposed to smooth the reference path, and then search the look-ahead point according to the geometric relationship between the vehicle and the reference path: An improved search algorithm based on vehicle speed and road curvature was used to search the look-ahead point. If the reference path curvature changes too much, the preliminarily obtained look-ahead point are modified, and finally the improved control algorithm (Improve Pure Pursuit Method (IPP) was obtained to calculate the steering Angle of the front wheel. Subsequently, the Carsim/Simulink co-simulation model and the autonomous vehicle platform were established for verification. Simulation and real Vehicle verification show that, compared with the pure pursuit algorithm based on Vehicle speed control (VPP) and Fuzzy Logic control pure Pursuit (FPP), IPP can perform better in complex low-speed lateral tracking scenes, reduce the problem of cutting-corner when the vehicle enters a corner, and improve the lateral tracking accuracy.
Isolation Of Bacterial Strains From Compost Teas And Screening Of Their PGPR Properties
Authors: Samet Mariem , Ghazala Imen , Karray Fatma , Abid Cyrine , Chiab Nour , Nouri-Ellouze Oumèma , Sayadi Sami , Gargouri-Bouzid Radhia 
Published: February 2022

Abstract
The beneficial effect of compost and compost tea on plant growth and protection is mainly associated with the microbial diversity and the presence of bacteria with Plant Growth Promoting effect. PGPR are considered as eco-friendly bio-fertilizers that may reduce the use of chemical pesticides and fertilizers. Three Composts (AT, A10 and A30) were previously prepared from industrial wastes (olive mill wastewater, olive pomace, coffee ground and phosphogypsum). In the present study we isolated three bacterial strains from the compost teas. The phylogenetic identification of these bacterial strains (B.AT, B.A10 and B.A30) showed that they were affiliated with Serratia plymuthica (B.AT), Serratia grimesii (B.A10) and Achromobacter piechaudii (B.A30) species. A characterization of the PGPR traits of these three bacteria was carried out in vitro (Siderophore, Exopolysaccharides, IAA production…) and the study of their effect on potato plants growth, yields and tuber quality was studied under field culture conditions.