bluuebunny/crossref_metadata_2025_split huggingface dataset:
Possibilities of using Biolactase L20 to reduce the concentration of lactose in probiotic beverages
Authors: Rodionova N S , Razinkova T A , Popov E S , Pozhidaeva E A , Sholin V A , Gubarev D A 
Published: July 2022

Abstract
Probiotic fermented milk products are most in demand at present due to the need for the prevention of new viral diseases and rehabilitation after previous diseases. Low-lactose probiotic products are especially relevant at present for people suffering from lactase deficiency, as carriers of probiotics, the restoration of which is necessary for various pathologies from the immune system or the gastrointestinal tract. The results of experimental studies of reducing the mass fraction of lactose in the preparation of probiotic fermented milk drinks on skimmed milk using a combination of the consortia Str. thermophiles, L. acidophilus, L. plantarum, L. fermentum and Str. thermophiles, B. bifidum, B. longum, B. Adolescentis and the enzyme preparation Biolactase L20 are presented. It is shown that the introduction of Biolactase L20 into skimmed milk at 34 oC reduces the lactose concentration by 78 % in 8 hours. An increase or decrease in temperature by more than 1.0-1.5 oC reduces the efficiency of lactose hydrolysis. The studied consortia of probiotic microorganisms showed the possibility of growth at this temperature until the titratable acidity of 110-130oT is reached, casein coagulation, and the formation of a dense clot in 14-16 hours. At the same time, the decrease in the lactose concentration was up to 30 %. The combination of Biolactase L20 and lactic acid fermentation with these consortia allowed one to reduce the lactose concentration by 77-78 %, which corresponded to 1.0-1.1 %. At the same time, a more active growth of lactobacilli was noted, the achieved microbial number was 7-8; the fermentation time to achieve coagulation and the minimum lactose concentration was 6 hours, for a consortium of bifidobacteria – 8 hours with equal product characteristics.
Optimization of the composition of the protein-carbohydrate base of the nutrient medium for the production of monospecific bacterial starter culture of Lactobacillus casei
Authors: Sviridenko G M , Mamykin D S , Komarova T V 
Published: July 2022

Abstract
Mesophilic bacteria of Lactobacillus casei are used in the food industry not only to accelerate the cheese ripening, but also as a probiotic culture in functional foods. This article describes the results of studies to optimize the composition of the nutrient medium for the cultivation of mesophilic lactobacilli of Lbc. casei. The use of cheese whey as the basis of a nutrient medium to ensure the required level of energy metabolism leads to a decrease in production costs. However, cheese whey contains an insufficient amount of protein components and growth factors for the development of Lbc. casei. To solve the problem of providing mesophilic lactobacilli with adequate nutrition, cheese whey was enriched with an enzymatic milk protein hydrolyzates and yeast autolysate as a source of biologically active substances that stimulate the growth of lactobacilli. The optimized composition of the protein-carbohydrate base of the nutrient medium makes it possible to increase the biomass yield during deep liquid-phase cultivation and the initial infection dose of 1 % for 24 hours of cultivation to obtain the number of viable cells of Lbc. casei at the level of (8.5±0.2)⋅108 CFU/cm3, and as a consequence, to increase the efficiency of production of monospecific bacterial starter cultures.
Mass transfer processes modeling in the production of dried and air-dried fish
Authors: Ershov M A , Ershov A M , Votinov M V 
Published: July 2022

Abstract
The focus areas for the Fisheries industry in Russia are increasing the share of products with high added value and development of the domestic consumer market of fishery products. In the Russian Federation the products made of aquatic bioresources, such as stock fish, air-dried and dried fish, are traditionally in strong demand with the population. It has been determined, that the manufacture of these fish products remains at a low level. In order to increase the volume of dried and stock fish production, improve the product’s quality specifications, it is necessary to develop the technology of the drying process, as well as to find the ways and methods to decrease the drying time. The efficiency of these productions can be increased by using the scientific-based process solutions that allow to keep the production costs down, improve the consumer properties of the end products and ensure its safety. The generalized dependences for calculating the duration of dehydration in production of air-dried and stock fish products have been developed in this study. The generalization has been performed based on the ratio of the current moisture content of fish to the first and second critical moistures of the kinetics curves of dehydration. A formula for calculating the current moisture content depending on the duration of the dehydration process and the dependence for calculating the drying time in relation to fish moisture have been introduced. The patterns received allow to model the kinetics curves of fish dehydration. A functional relation between the critical moistures of the kinetics of dehydration, the water diffusion coefficients and the initial moisture content has been determined. The derived dependencies allow to calculate the diffusion coefficients in the critical points of the kinetics curve of dehydration. The obtained formulas could be used for calculating the water diffusion coefficients in fish. Using the suggested formulas makes it possible to solve the diffuse type partial differential equation. This gives an opportunity to model the water distribution in fish during the process of dehydration.
Production of butter paste with dihydroquercetin technology
Authors: Ziablitseva M A , Bessonova J A , Yershova O V 
Published: July 2022

Abstract
The study proposes a technology for producing butter paste with dihydroquercetin. For the production of butter paste with dihydroquercetin, a method of converting high-fat cream has been chosen. Organoleptic, physico-chemical characteristics of the quality of raw materials have been studied. The technology of butter paste with dihydroquercetin was developed. Butter paste with dihydroquercetin was obtained as follows. The quality of raw milk and dihydroquercetin was assessed. Milk was separated, and the resulting cream was pasteurized. Further, high-fat cream was obtained by secondary separation, and their normalization was carried out. Then, dihydroquercetin was added to the normalized mixture according to the recipe. The normalized mixture was converted into butter paste and directed to thermostatting. Butter paste with dihydroquercetin is a low fat product with functional properties. The product is intended for systematic use by the population of all age groups.
The development of formulation for curd products based on the methodology of risk qualimetry
Authors: Yankovskaya V S , Dunchenko N I , Voloshina E S , Kuptsova S V , Mikhaylova K V , Ginzburg M A 
Published: July 2022

Abstract
The authors suggest a new approach to the development of curd products formulation. The approach is based on the methodology of "risk qualimetry" which uses information-matrix models. Defect names are revealed and ranked by the detection rate, as well as reasons for the complaints about the curd products quality that had been coming from retail chains within 5 years. Moreover, the information-matrix model of the influence of the recipe ingredients on the risks of complaints about the curd product quality was presented. The values of the importance indicators were determined and the formulation factors were ranked: low-fat cottage cheese (185.8 points), a structurant (153.9), a functional ingredient (100.3), sugar syrup (60.0) and cream (54.2). The choice of a functional component and a structure former was substantiated. Those components would not only increase the nutritional value of the product, but also minimize the risks of inconsistencies. The formulation of a new curd product with functional components was developed: fat-free curd (65.0%), cream (24.3%), sugar syrup (8.0%), oats cryopowder (1.2%) and red grapes cryopowder (1.5%). The organoleptic evaluation of the prototype model showed high consumer properties.
The use of spectrophotometry to control the quality of edible rapeseed oil in the process of adsorption
Authors: Peregonchaya O V , Korol'kova N V , Sokolova S A , D'yakonova O V , Tolmachev V D 
Published: July 2022

Abstract
Comparison of spectral characteristics made it possible to establish the carotenoid composition. The presence of chlorophyll in rapeseed oil has been established. A comparison of the spectrograms of unrefined, refined oils and a regenerating solvent solution in the range of 200-700 nm is carried out. Almost complete removal of carotenoids from rapeseed oil and the absence of absorption of spectrally active components by the sorbent in the range of 200-350 nm are shown. Such substances can include glucosinolates, tocopherols.
The choice of rational modes of vacuum deposition of fatty components in the preparation of feed for valuable fish species
Authors: Bogomolov I S , Afanasyev V A , Ostrikov A N , Startseva S V 
Published: July 2022

Abstract
The subject of research is kinetics of vacuum deposition of liquid components on extruded fish feed pellets. The kinetic regularities of the vacuum deposition process under investigation are established and the choice of rational modes of vacuum deposition of fat components in preparing compound feeds for valuable fish species is justified. The use of vacuum deposition of fat components on extruded feed for valuable fish species has led to the possibility of increasing the fat content up to 40 %, reducing the crushing of feed by 10 %. Improving the physical characteristics of finished compound feed (a moisture content of 10-12 %); increasing the penetration level of liquid components by 25-30 %; maintaining a dry surface of the extrudate; reducing pathogenic microflora to 0 are included. It was revealed that the amount of vacuum in working chamber of sprayer is the most important parameter affecting a fat leakage factor, and the amount of vacuum of 0.020 MPa provides intensive penetration and significantly reduces a fat leakage factor.
Development of process for obtaining crystalline food products with improved granulometric composition
Authors: Vorontsov V V , Shakhova M N , Butova S V 
Published: July 2022

Abstract
The subject of this research is the process of isohydric crystallization of mass from solution in a circulating crystallizer with the ability to control the size of food crystals. The developed design of a continuous circulation crystallizer provides the opportunity to adjust the circulation rate and meet temperature conditions. The functions of the solution particles and solid phase distribution over the time of residence in the crystallizer filled with a circulating suspension are found for the modes of ideal mixing and displacement determined by the circulation ratio of the suspension in the apparatus. The ability to control solution supersaturation and the time of suspension particles residence in the apparatus enables to obtain large production crystals.
Biotechnologies used to deal with recycling of grape pomace
Authors: Ageeva N M , Tikhonova A N , Globa E V 
Published: July 2022

Abstract
The article presents experimental data on the chemical composition of grape pomace subject to the used grape processing technology. Grape pomace is shown to contain a rich complex of biologically valuable compounds, including polyphenols (with antioxidant activity), polysaccharides (including pectin substances), and nitrogenous substances which serve as the substrate for fermentative catalysis and development of microorganisms. It has been established that the microorganisms of Baikal EM-1 preparation most actively developed on sweet pomace of white and red grape varieties. When Baikal EM-1 was inoculated onto the pomace surface, the number of microorganisms using mineral forms of nitrogen grew substantially, and the number of free amino acids triples. It was fungi of Trichoderma and Rhisopus genera that were developing most actively on all types of grape pomace, including that of red grapes. The effect of the fungi was accompanied by a fast change in the structure of the pomace – viz., appearance of the liquid and slimy phases. An active complex of enzymes of cellulolytic and hemicellulasic activity was shown to ensure a deeper level of maceration of the vegetative tissue of pomace, enabling to perfect its processing technology.
Improving the efficiency of the bee-bread drying process
Authors: Oskin S V , Kharchenko S N , Tsokur D S 
Published: July 2022

Abstract
Drying is one of the most common operations in the processing of agricultural products. Improvement of installations for its implementation is an important task, especially in beekeeping in the drying of bee-bread. The article deals with the problem of uneven drying of bee-bread in trays when using convective installations. A method for solving this problem by using trays with holes is proposed. The results of modeling the processes occurring in the drying chamber during the drying of the traditional dryer and the modernized one are shown. Comparison of the results confirmed the reduction of the difference in moisture content between the upper and lower trays in the process of drying by a factor of 3.
Determination of the limiting stage of the adsorption kinetics of the dye “Methylene Blue” by the biomass of apricot pits
Authors: Vinogradenko Yu A , Sverguzova S V , Shaikhiev I G , Zhabskiy V V , Ievleva E S 
Published: July 2022

Abstract
The paper presents the results of experimental studies to determine the limiting stage of the adsorption kinetics of the methylene blue dye by the biomass of the crushed peel of apricot pits. Morphological studies of the surface of the peel of apricot pits were carried out using a scanning electron microscope of the brand “TESCAN MIRA 3 LMU” (Czech Republic). Adsorption studies were carried out under static conditions by treating the biomass of the apricot pit peel with solutions of methylene blue of specified concentrations for a certain time. On the basis of the obtained data, the kinetic dependences were constructed and the necessary indicators were calculated. It was found that the sorption capacity of the apricot pit biomass under experimental conditions is 0.565 mmol/g. The results of the experiment were processed using the Microsoft office Excel software in the framework of the diffusion model, and an intradiffusion kinetic model of sorption was obtained. The constants of the intradiffusion kinetic model of sorption and the parameters of the internal diffusion coefficient are calculated, and the contribution of external diffusion to the adsorption process is determined. It is revealed that the adsorption process is limited by external diffusion.
Detection of causes of defects in the cooked sausage production
Authors: Voloshina E S , Dunchenko N I , Yankovskaya V S , Kuptsova S V , Mikhaylova K V , Odintsova A A 
Published: July 2022

Abstract
The work presents a method for analysing hazardous factors in the cooked sausage production. The method includes the formation of a register of hazardous factors and the determination of cause-and-effect relationships between the hazards and their causes. The formed register includes 30 indicators and 25 factors affecting the occurrence of defects in cooked sausages. The critical control points of the cooked sausage production technology were determined, and a hazard analysis was carried out for each critical control point. In integration with the principles of HACCP, the developed method helps to reduce the number of defective products and increase customers` satisfaction.
Study of the amino acid composition of beef proteins using ionizing radiation treatment technology
Authors: Timakova R T , Iliukhina Iu V 
Published: July 2022

Abstract
The choice of modern technologies at the enterprises of the agroindustrial complex allows ensuring the safety and high quality indicators of food products. The spread of industrial ionizing radiation treatment technology requires compliance with procedural processes in specialized radiation centers. To ensure the safety and preservation of the quality of raw meat, in particular chilled beef, after treatment with ionizing radiation, it is of scientific and practical importance to substantiate the radiation doses used and to regulate the absorbed radiation doses, the calculation of which is carried out according to the proposed methodology. After treatment with ionizing radiation in all samples of chilled beef, the processing efficiency was 100%. A decrease in the total amino acid content was found, lower amino acid losses were found in beef samples treated with a dose of ionizing radiation up to 10 kGy, including for essential amino acids - by 0.6% and for interchangeable amino acids - by 1.1%. The limiting amino acid has not been established, which makes it possible to provide daily coverage of the need for amino acids. As a result of the study of the balance of the amino acid composition by ion exchange chromatography and organoleptic parameters, the optimal dose of ionizing radiation of 10 kGy for processing chilled beef was established.
Predicting Need for Escalation of Care or Death From Repeated Daily Clinical Observations and Laboratory Results in Patients With Severe Acute Respiratory Syndrome Coronavirus 2
Authors: Crooks Colin J , West Joe , Fogarty Andrew , Morling Joanne R , Grainge Matthew J , Gonem Sherif , Simmonds Mark , Race Andrea , Juurlink Irene , Briggs Steve , Cruickshank Simon , Hammond-Pears Susan , Card Timothy R 
Published: July 2022

Abstract
We compared the performance of prognostic tools for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using parameters fitted either at the time of hospital admission or across all time points of an admission. This cohort study used clinical data to model the dynamic change in prognosis of SARS-CoV-2 at a single hospital center in the United Kingdom, including all patients admitted from February 1, 2020, to December 31, 2020, and then followed up for 60 days for intensive care unit (ICU) admission, death, or discharge from the hospital. We incorporated clinical observations and blood tests into 2 time-varying Cox proportional hazards models predicting daily 24- to 48-hour risk of admission to the ICU for those eligible for escalation of care or death for those ineligible for escalation. In developing the model, 491 patients were eligible for ICU escalation and 769 were ineligible for escalation. Our model had good discrimination of daily risk of ICU admission in the validation cohort (n = 1,141; C statistic: C = 0.91, 95% confidence interval: 0.89, 0.94) and our score performed better than other scores (National Early Warning Score 2, International Severe Acute Respiratory and Emerging Infection Comprehensive Clinical Characterisation Collaboration score) calculated using only parameters measured on admission, but it overestimated the risk of escalation (calibration slope = 0.7). A bespoke daily SARS-CoV-2 escalation risk prediction score can predict the need for clinical escalation better than a generic early warning score or a single estimation of risk calculated at admission.
Detecting retinal neural and stromal cell classes and ganglion cell subtypes based on transcriptome data with deep transfer learning
Authors: Madadi Yeganeh , Sun Jian , Chen Hao , Williams Robert , Yousefi Siamak 
Published: July 2022

AbstractMotivationTo develop and assess the accuracy of deep learning models that identify different retinal cell types, as well as different retinal ganglion cell (RGC) subtypes, based on patterns of single-cell RNA sequencing (scRNA-seq) in multiple datasets.ResultsDeep domain adaptation models were developed and tested using three different datasets. The first dataset included 44 808 single retinal cells from mice (39 cell types) with 24 658 genes, the second dataset included 6225 single RGCs from mice (41 subtypes) with 13 616 genes and the third dataset included 35 699 single RGCs from mice (45 subtypes) with 18 222 genes. We used four loss functions in the learning process to align the source and target distributions, reduce misclassification errors and maximize robustness. Models were evaluated based on classification accuracy and confusion matrix. The accuracy of the model for correctly classifying 39 different retinal cell types in the first dataset was ∼92%. Accuracy in the second and third datasets reached ∼97% and 97% in correctly classifying 40 and 45 different RGCs subtypes, respectively. Across a range of seven different batches in the first dataset, the accuracy of the lead model ranged from 74% to nearly 100%. The lead model provided high accuracy in identifying retinal cell types and RGC subtypes based on scRNA-seq data. The performance was reasonable based on data from different batches as well. The validated model could be readily applied to scRNA-seq data to identify different retinal cell types and subtypes.Availability and implementationThe code and datasets are available on https://github.com/DM2LL/Detecting-Retinal-Cell-Classes-and-Ganglion-Cell-Subtypes. We have also added the class labels of all samples to the datasets.Supplementary informationSupplementary data are available at Bioinformatics online.
Case report: mRNA COVID-19 vaccine-related acute pericarditis with evolution to myopericarditis
Authors: Riddell Craig , Edwards Nicola C , Stewart Ralph 
Published: July 2022

Abstract

Background
Both acute pericarditis and myocarditis have been reported as rare complications following vaccination with the Pfizer-Biotech and Moderna mRNA COVID-19 vaccines.

Case summary
An 18-year-old man presented with clinical and electrocardiographic changes of acute pericarditis 2 days after receiving the second dose of the BNT162b2 (Pfizer–BioNTech) vaccine. His electrocardiogram also showed an incomplete right bundle branch block. Troponin T on presentation was normal (reference &lt;14 ng/L) but subsequently increased to a peak 1080 ng/L by day 4 post vaccination. Evolving electrocardiographic changes and cardiac MRI findings were consistent with acute myopericarditis.

Discussion
This patient’s clinical course was uncomplicated, which is consistent with studies indicating that post-COVID vaccine myocarditis usually has a mild course with a low chance of arrhythmia or heart failure. Troponin elevation is a part of the diagnostic criteria for myocarditis. This case is consistent with another report demonstrating that troponin levels can be within the normal range early in the clinical course of post-COVID vaccine myopericarditis. The incomplete right bundle branch block resolved by day 4 post-vaccination and thus may have represented early myocardial involvement at presentation. Further testing and monitoring should be considered in patients who present soon after COVID-19 mRNA vaccination with pericarditis features or minor conduction delays, in order to rule out progression to myopericarditis. Identifying myocardial involvement is clinically relevant as it indicates a risk of developing arrhythmia or heart failure, as well as having implications for physical activity advice and future booster vaccination.