bluuebunny/crossref_metadata_2025_split huggingface dataset:
Finger millet-based muffin decreases insulin response in individuals with prediabetes in a randomised controlled trial
Authors: Almaski Ameerah , Coe Shelly , Lightowler Helen , Clegg Miriam E , Thondre Pariyarath Sangeetha 
Published: May 2022

AbstractMillet is a grain high in polyphenols and antioxidants, which are bioactive compounds known to influence blood glucose response. The aim of this study was to compare the effect of finger millet muffin and wheat muffin on glycaemic response (GR), insulin response (IR), gastric emptying (GE) and satiety in healthy individuals and people with prediabetes. In a single-blind randomised controlled crossover trial at Oxford Brookes Centre for Nutrition and Health, fifteen healthy individuals and fourteen individuals with prediabetes were recruited between May and December 2017. The participants’ GR (3 h), IR (3 h), GE (4 h) and satiety (4 h) were measured before and after the consumption of muffins. A mixed method ANOVA was used to compare GE and the incremental AUC (iAUC) for GR and IR between the participant groups and muffins. There was a significant interaction between participants and muffins on IR iAUC at 180 min (P = 0·042). A significant effect of muffins was found on the GR peak (P = 0·013). The millet muffin decreased the GR peak and IR iAUC compared with the wheat muffin in participants with prediabetes. A significant interaction between participants and muffins for GE ascension time Tasc (P = 0·017) was observed, with no effect of muffins on satiety AUC in the participant groups. This study suggested that polyphenol and fibre-rich finger millet may have the potential to influence the management of prediabetes.
On the terminology designating the Zoroastrians of Iran and their language
Authors: Gholami Saloumeh 
Published: May 2022

AbstractThis paper* examines how Zoroastrians designate themselves (internal/self-designations), and how they are designated by others (external designations). Focusing on the termGabr/Gavras the external denotation for Zoroastrians and the termGabrī/Gavrūnīas the designation for their language, it argues that these terms, once common in Western scholarship as well as among non-Zoroastrian Iranians, have become obsolete due to their pejorative undertones. However, they have recently been revived by some scholars, who justify such use with reference to the alleged etymology ofGabras meaning “man” and by the fact that even some Zoroastrians useGavr/GavrūnandGavrī/Gavrūnīas an internal designation for themselves and their language. This paper critically examines these views and argues that neither the etymology nor the internal self-designation justifies the use of these terms and proposes the termZoroastrian Darīas the more appropriate designation of the language of the Zoroastrians of Iran.
Can We Algorithmize Politics? The Promise and Perils of Computerized Text Analysis in Political Research
Authors: Mitrani Mor , Adams Tracy , Noy Inbar 
Published: May 2022

ABSTRACTIn recent years, political scientists increasingly have used data-science tools to research political processes, positions, and behaviors. Because both domestic and international politics are grounded in oral and written texts, computerized text analysis (CTA)—typically based on natural-language processing—has become one of the most notable applications of data-science tools in political research. This article explores the promises and perils of using CTA methods in political research and, specifically, the study of international relations. We highlight fundamental analytical and methodological gaps that hinder application and review processes. Whereas we acknowledge the significant contribution of CTA to political research, we identify a dual “engagement deficit” that may distance those without prior background in data science: (1) the tendency to prioritize methodological innovation over analytical and theoretical insights; and (2) the scholarly and political costs of requiring high proficiency levels and training to comprehend, assess, and use advanced research models.
Association between health care access and food insecurity among lower-income older adults with multiple chronic conditions in Washington State, USA
Authors: Hill Courtney M , Tseng Ashley S , Holzhauer Katherine , Littman Alyson J , Jones-Smith Jessica C 
Published: May 2022

AbstractObjective:Lower-income older adults with multiple chronic conditions (MCC) are highly vulnerable to food insecurity. However, few studies have considered how health care access is related to food insecurity among older adults with MCC. The aims of this study were to examine associations between MCC and food insecurity, and, among older adults with MCC, between health care access and food insecurity.Design:Cross-sectional study data from the 2019 Behavioral Risk Factor Surveillance System survey.Setting:Washington State, USA.Participants:Lower-income adults, aged 50 years or older (n 2118). MCC was defined as having ≥ 2 of 11 possible conditions. Health care access comprised three variables (unable to afford seeing the doctor, no health care coverage and not having a primary care provider (PCP)). Food insecurity was defined as buying food that did not last and not having money to get more.Results:The overall prevalence of food insecurity was 26·0 % and was 1·50 times greater (95 % CI 1·16, 1·95) among participants with MCC compared to those without MCC. Among those with MCC (n 1580), inability to afford seeing a doctor was associated with food insecurity (prevalence ratio (PR) 1·83; 95 % CI 1·46, 2·28), but not having health insurance (PR 1·49; 95 % CI 0·98, 2·24) and not having a PCP (PR 1·10; 95 % CI 0·77, 1·57) were not.Conclusions:Inability to afford healthcare is related to food insecurity among older adults with MCC. Future work should focus on collecting longitudinal data that can clarify the temporal relationship between MCC and food insecurity.
Dementia-related continuing education for rural interprofessional primary health care in Saskatchewan, Canada: perceptions and needs of webinar participants
Authors: Kosteniuk Julie , Morgan Debra , O’Connell Megan E. , Seitz Dallas , Elliot Valerie , Bayly Melanie , Cameron Chelsie , Froehlich Chow Amanda 
Published: May 2022

Abstract
Dementia-related continuing education opportunities are important for rural primary health care (PHC) professionals given scarce specialized resources. This report explores the initial perceptions and continuing education needs of rural interprofessional memory clinic team members and other PHC professionals related to a short series of dementia-related education webinars. Three webinars on separate topics were delivered over an 8-month period in 2020 in Saskatchewan, Canada. The research design involved analysis of webinar comments and post-webinar survey data. Sixty-eight individuals participated in at least one webinar, and 46 surveys were completed. Rural memory clinic team members accounted for a minority of webinar participants and a majority of survey respondents. Initial perceptions were positive, with webinar topics and interactivity identified as the most effective aspects. Continuing education needs were mainly aligned with professional roles; however, some overlap of interests occurred. Future webinars will further explore learning needs within an interprofessional environment.
Elongated Magma Plumbing System Beneath the Coso Volcanic Field, California, Constrained by Seismic Reflection Tomography
Authors: Wang Dongdong , Wu Shucheng , Li Tianjue , Tong Ping , Gao Yongxin 
Published: May 2022

AbstractThe magma plumbing in the lower crust beneath the Coso volcanic field (CVF) remains controversial, largely because of the absence of high‐resolution lower crustal velocity models. For the first time, we develop a high‐resolution crustal P‐wave velocity model for the Coso‐Ridgecrest region by jointly inverting 137,992 first P and 8,636 PmP travel‐time data using an eikonal equation‐based seismic reflection tomography method. More than half of the PmP travel times are picked from earthquakes after the 2019 Mw 7.1 Ridgecrest earthquake. Such abundant PmP travel times significantly improve the resolution of the lower crust. Our final velocity model reveals a prominent low‐velocity body sitting right beneath the CVF at 5–20 km depths, which we interpret as a rhyolite magma reservoir that supplies heat flux to the hot springs and also feeds the volcanic activities at Coso. We find that the upper‐middle crustal low‐velocity body dips southwards into the lower crust, extending to regions beneath the Indian Wells Valley and the Garlock Fault at depth greater than 20 km. We ascribe the lower crustal low‐velocity body (more than 4% Vp reduction) to a basaltic magma reservoir that connects the melts in the uppermost mantle with the eruptible rhyolitic reservoir at shallower depths. The basaltic magma reservoir constitutes an important part of a continuous N‐S elongated crustal magma plumbing system beneath the CVF, formed as a combined result of local extension, faulting, and stress distribution.
Dissolved and Particulate Barium Distributions Along the US GEOTRACES North Atlantic and East Pacific Zonal Transects (GA03 and GP16): Global Implications for the Marine Barium Cycle
Authors: Rahman Shaily , Shiller Alan M. , Anderson Robert F. , Charette Matthew A. , Hayes Christopher T. , Gilbert Melissa , Grissom Karen R. , Lam Phoebe J. , Ohnemus Daniel C. , Pavia Frank J. , Twining Benjamin S. , Vivancos Sebastian M. 
Published: May 2022

AbstractProcesses controlling dissolved barium (dBa) were investigated along the GEOTRACES GA03 North Atlantic and GP16 Eastern Tropical Pacific transects, which traversed similar physical and biogeochemical provinces. Dissolved Ba concentrations are lowest in surface waters (∼35–50 nmol kg−1) and increase to 70–80 and 140–150 nmol kg−1 in deep waters of the Atlantic and Pacific transects, respectively. Using water mass mixing models, we estimate conservative mixing that accounts for most of dBa variability in both transects. To examine nonconservative processes, particulate excess Ba (pBaxs) formation and dissolution rates were tracked by normalizing particulate excess 230Th activities. Th‐normalized pBaxs fluxes, with barite as the likely phase, have subsurface maxima in the top 1,000 m (∼100–200 μmol m−2 year−1 average) in both basins. Barite precipitation depletes dBa within oxygen minimum zones from concentrations predicted by water mass mixing, whereas inputs from continental margins, particle dissolution in the water column, and benthic diffusive flux raise dBa above predications. Average pBaxs burial efficiencies along GA03 and GP16 are ∼37% and 17%–100%, respectively, and do not seem to be predicated on barite saturation indices in the overlying water column. Using published values, we reevaluate the global freshwater dBa river input as 6.6 ± 3.9 Gmol year−1. Estuarine mixing processes may add another 3–13 Gmol year−1. Dissolved Ba inputs from broad shallow continental margins, previously unaccounted for in global marine summaries, are substantial (∼17 Gmol year−1), exceeding terrestrial freshwater inputs. Revising river and shelf dBa inputs may help bring the marine Ba isotope budget more into balance.
Spatio‐Temporal Complexity of Aftershocks in the Apennines Controlled by Permeability Dynamics and Decarbonization
Authors: Gunatilake Thanushika , Miller Stephen A. 
Published: May 2022

AbstractIn 2016, a series of large normal faulting earthquakes in the Apennines filled the seismic gap between the 1997 Colfiorito and 2009 L’Aquila earthquakes. These earthquakes, known as the Amatrice‐Visso‐Norcia (AVN) sequence, spawned hundreds of thousand of aftershocks in the first year, extending about 60 km along strike. The Colfiorito and L’Aquila aftershocks showed a significant high fluid‐pressure component in their aftershock genesis, and here we show evidence that the AVN aftershocks also include a significant high pressure CO2 component. This CO2 is both deeply‐derived, and internally generated by thermal decomposition. Using a simple model of non‐linear diffusion with a source term, we compare model results with the observed cumulative number of aftershocks, and also spatial comparisons between the calculated fluid pressure and the hypocenters of about 35,000 well‐located events. The good comparisons between model and observations provide evidence that the AVN sequence includes a contribution (additional fluid source) from co‐seismic decarbonization. Our results suggest that internal fluid generation through devolitization or decarbonization is responsible for non‐Omori type aftershock behavior such as observed for the AVN sequence and for the 2011 Mw 9.1 Tohoku (Japan) and the 2014 Iquique (Chile) Mw 8.2 megathrust subduction zone earthquakes.
Systematic characterization of cleanroom-free fabricated macrovalves, demonstrating pumps and mixers for automated fluid handling tuned for organ-on-chip applications
Authors: Bossink Elsbeth G. B. M. , Vollertsen Anke R. , Loessberg-Zahl Joshua T. , van der Meer Andries D. , Segerink Loes I. , Odijk Mathieu 
Published: May 2022

AbstractIntegrated valves enable automated control in microfluidic systems, as they can be applied for mixing, pumping and compartmentalization purposes. Such automation would be highly valuable for applications in organ-on-chip (OoC) systems. However, OoC systems typically have channel dimensions in the range of hundreds of micrometers, which is an order of magnitude larger than those of typical microfluidic valves. The most-used fabrication process for integrated, normally open polydimethylsiloxane (PDMS) valves requires a reflow photoresist that limits the achievable channel height. In addition, the low stroke volumes of these valves make it challenging to achieve flow rates of microliters per minute, which are typically required in OoC systems. Herein, we present a mechanical ‘macrovalve’ fabricated by multilayer soft lithography using micromilled direct molds. We demonstrate that these valves can close off rounded channels of up to 700 µm high and 1000 µm wide. Furthermore, we used these macrovalves to create a peristaltic pump with a pumping rate of up to 48 µL/min and a mixing and metering device that can achieve the complete mixing of a volume of 6.4 µL within only 17 s. An initial cell culture experiment demonstrated that a device with integrated macrovalves is biocompatible and allows the cell culture of endothelial cells over multiple days under continuous perfusion and automated medium refreshment.
Transfer of microRNA-22-3p by M2 macrophage-derived extracellular vesicles facilitates the development of ankylosing spondylitis through the PER2-mediated Wnt/β-catenin axis
Authors: Liu Chong , Liang Tuo , Zhang Zide , Chen Jiarui , Xue Jang , Zhan Xinli , Ren Liang 
Published: May 2022

AbstractPathological osteogenesis and inflammation possess critical significance in ankylosing spondylitis (AS). The current study aimed to elucidate the mechanisms regarding extracellular vesicle (EV)-packaged microRNA-22-3p (miR-22-3p) from M2 macrophages in the osteogenic differentiation of mesenchymal stem cells (MSCs) in AS. EVs were initially isolated from M2 macrophages, which had been treated with either restored or depleted miR-22-3p. AS-BMSCs were subsequently treated with M2 macrophage-derived EVs to detect osteogenic differentiation in BMSCs using gain- or loss-of-function experiments. The binding affinity among miR-22-3p, period circadian protein 2 (PER2), and Wnt7b was identified. Finally, AS mouse models were established for testing the effects of M2-EV-miR-22-3p on the bone metastatic microenvironment in vivo. miR-22-3p from M2 macrophages could be transferred into BMSCs via EVs, which promoted the osteogenic differentiation of AS-BMSCs. miR-22-3p inhibited PER2, while PER2 blocked the Wnt/β-catenin signaling pathway via Wnt7b inhibition. M2-EV-shuttled miR-22-3p facilitated alkaline phosphatase activity and extracellular matrix mineralization via PER2-regulated Wnt/β-catenin axis, stimulating the BMSC osteogenic differentiation. Taken together, these findings demonstrate that miR-22-3p in M2 macrophage-released EVs downregulates PER2 to facilitate the osteogenesis of MSCs via Wnt/β-catenin axis.
Signal requirement for cortical potential of transplantable human neuroepithelial stem cells
Authors: Varga Balazs V. , Faiz Maryam , Pivonkova Helena , Khelifi Gabriel , Yang Huijuan , Gao Shangbang , Linderoth Emma , Zhen Mei , Karadottir Ragnhildur Thora , Hussein Samer M. , Nagy Andras 
Published: May 2022

AbstractThe cerebral cortex develops from dorsal forebrain neuroepithelial progenitor cells. Following the initial expansion of the progenitor cell pool, these cells generate neurons of all the cortical layers and then astrocytes and oligodendrocytes. Yet, the regulatory pathways that control the expansion and maintenance of the progenitor cell pool are currently unknown. Here we define six basic pathway components that regulate proliferation of cortically specified human neuroepithelial stem cells (cNESCs) in vitro without the loss of cerebral cortex developmental potential. We show that activation of FGF and inhibition of BMP and ACTIVIN A signalling are required for long-term cNESC proliferation. We also demonstrate that cNESCs preserve dorsal telencephalon-specific potential when GSK3, AKT and nuclear CATENIN-β1 activity are low. Remarkably, regulation of these six pathway components supports the clonal expansion of cNESCs. Moreover, cNESCs differentiate into lower- and upper-layer cortical neurons in vitro and in vivo. The identification of mechanisms that drive the neuroepithelial stem cell self-renewal and differentiation and preserve this potential in vitro is key to developing regenerative and cell-based therapeutic approaches to treat neurological conditions.
Structural insights into the mechanism of archaellar rotational switching
Authors: Altegoer Florian , Quax Tessa E. F. , Weiland Paul , Nußbaum Phillip , Giammarinaro Pietro I. , Patro Megha , Li Zhengqun , Oesterhelt Dieter , Grininger Martin , Albers Sonja-Verena , Bange Gert 
Published: May 2022

AbstractSignal transduction via phosphorylated CheY towards the flagellum and the archaellum involves a conserved mechanism of CheY phosphorylation and subsequent conformational changes within CheY. This mechanism is conserved among bacteria and archaea, despite substantial differences in the composition and architecture of archaellum and flagellum, respectively. Phosphorylated CheY has higher affinity towards the bacterial C-ring and its binding leads to conformational changes in the flagellar motor and subsequent rotational switching of the flagellum. In archaea, the adaptor protein CheF resides at the cytoplasmic face of the archaeal C-ring formed by the proteins ArlCDE and interacts with phosphorylated CheY. While the mechanism of CheY binding to the C-ring is well-studied in bacteria, the role of CheF in archaea remains enigmatic and mechanistic insights are absent. Here, we have determined the atomic structures of CheF alone and in complex with activated CheY by X-ray crystallography. CheF forms an elongated dimer with a twisted architecture. We show that CheY binds to the C-terminal tail domain of CheF leading to slight conformational changes within CheF. Our structural, biochemical and genetic analyses reveal the mechanistic basis for CheY binding to CheF and allow us to propose a model for rotational switching of the archaellum.
Origins of direction selectivity in the primate retina
Authors: Kim Yeon Jin , Peterson Beth B. , Crook Joanna D. , Joo Hannah R. , Wu Jiajia , Puller Christian , Robinson Farrel R. , Gamlin Paul D. , Yau King-Wai , Viana Felix , Troy John B. , Smith Robert G. , Packer Orin S. , Detwiler Peter B. , Dacey Dennis M. 
Published: May 2022

AbstractFrom mouse to primate, there is a striking discontinuity in our current understanding of the neural coding of motion direction. In non-primate mammals, directionally selective cell types and circuits are a signature feature of the retina, situated at the earliest stage of the visual process. In primates, by contrast, direction selectivity is a hallmark of motion processing areas in visual cortex, but has not been found in the retina, despite significant effort. Here we combined functional recordings of light-evoked responses and connectomic reconstruction to identify diverse direction-selective cell types in the macaque monkey retina with distinctive physiological properties and synaptic motifs. This circuitry includes an ON-OFF ganglion cell type, a spiking, ON-OFF polyaxonal amacrine cell and the starburst amacrine cell, all of which show direction selectivity. Moreover, we discovered that macaque starburst cells possess a strong, non-GABAergic, antagonistic surround mediated by input from excitatory bipolar cells that is critical for the generation of radial motion sensitivity in these cells. Our findings open a door to investigation of a precortical circuitry that computes motion direction in the primate visual system.
Complex dynamics in a synchronized cell-free genetic clock
Authors: Aufinger Lukas , Brenner Johann , Simmel Friedrich C. 
Published: May 2022

AbstractComplex dynamics such as period doubling and chaos occur in a wide variety of non-linear dynamical systems. In the context of biological circadian clocks, such phenomena have been previously found in computational models, but their experimental study in biological systems has been challenging. Here, we present experimental evidence of period doubling in a forced cell-free genetic oscillator operated in a microfluidic reactor, where the system is periodically perturbed by modulating the concentration of one of the oscillator components. When the external driving matches the intrinsic period, we experimentally find period doubling and quadrupling in the oscillator dynamics. Our results closely match the predictions of a theoretical model, which also suggests conditions under which our system would display chaotic dynamics. We show that detuning of the external and intrinsic period leads to more stable entrainment, suggesting a simple design principle for synchronized synthetic and natural genetic clocks.
Prediction of protein–ligand binding affinity from sequencing data with interpretable machine learning
Authors: Rube H. Tomas , Rastogi Chaitanya , Feng Siqian , Kribelbauer Judith F. , Li Allyson , Becerra Basheer , Melo Lucas A. N. , Do Bach Viet , Li Xiaoting , Adam Hammaad H. , Shah Neel H. , Mann Richard S. , Bussemaker Harmen J. 
Published: May 2022

AbstractProtein–ligand interactions are increasingly profiled at high throughput using affinity selection and massively parallel sequencing. However, these assays do not provide the biophysical parameters that most rigorously quantify molecular interactions. Here we describe a flexible machine learning method, called ProBound, that accurately defines sequence recognition in terms of equilibrium binding constants or kinetic rates. This is achieved using a multi-layered maximum-likelihood framework that models both the molecular interactions and the data generation process. We show that ProBound quantifies transcription factor (TF) behavior with models that predict binding affinity over a range exceeding that of previous resources; captures the impact of DNA modifications and conformational flexibility of multi-TF complexes; and infers specificity directly from in vivo data such as ChIP-seq without peak calling. When coupled with an assay called KD-seq, it determines the absolute affinity of protein–ligand interactions. We also apply ProBound to profile the kinetics of kinase–substrate interactions. ProBound opens new avenues for decoding biological networks and rationally engineering protein–ligand interactions.