bluuebunny/crossref_metadata_2025_split huggingface dataset:
Porcine endometrial heat shock proteins are differentially influenced by pregnancy status, heat stress, and altrenogest supplementation during the peri-implantation period
Authors: Adur Malavika K , Seibert Jacob T , Romoser Matthew R , Bidne Katie L , Baumgard Lance H , Keating Aileen F , Ross Jason W 
Published: July 2022

AbstractHeat stress (HS) deleteriously affects multiple components of porcine reproduction and is causal to seasonal infertility. Environment-induced hyperthermia causes a HS response (HSR) typically characterized by increased abundance of intracellular heat shock proteins (HSP). Gilts exposed to HS during the peri-implantation period have compromised embryo survival, however if (or how) HS disrupts the porcine endometrium is not understood. Study objectives were to evaluate the endometrial HSP abundance in response to HS during this period and assess the effect of oral progestin (altrenogest; ALT) supplementation. Postpubertal gilts (n = 42) were artificially inseminated during behavioral estrus (n = 28) or were kept cyclic (n = 14), and randomly assigned to thermal neutral (TN; 21 ± 1 °C) or diurnal HS (35 ± 1 °C for 12 h/31.6 ± 1 °C for 12 h) conditions from day 3 to 12 postestrus (dpe). Seven of the inseminated gilts from each thermal treatment group received ALT (15 mg/d) during this period. Using quantitative PCR, transcript abundance of HSP family A (Hsp70) member 1A (HSPA1A, P = 0.001) and member 6 (HSPA6, P &lt; 0.001), and HSP family B (small) member 8 (HSB8, P = 0.001) were increased while HSP family D (Hsp60) member 1 (HSPD1, P = 0.01) was decreased in the endometrium of pregnant gilts compared to the cyclic gilts. Protein abundance of HSPA1A decreased (P = 0.03) in pregnant gilt endometrium due to HS, while HSP family B (small) member 1 (HSPB1) increased (P = 0.01) due to HS. Oral ALT supplementation during HS reduced the transcript abundance of HSP90α family class B member 1 (HSP90AB1, P = 0.04); but HS increased HSP90AB1 (P = 0.001), HSPA1A (P = 0.02), and HSPA6 (P = 0.04) transcript abundance irrespective of ALT. ALT supplementation decreased HSP90α family class A member 1 (HSP90AA1, P = 0.001) protein abundance, irrespective of thermal environment, whereas ALT only decreased HSPA6 (P = 0.02) protein abundance in TN gilts. These results indicate a notable shift of HSP in the porcine endometrium during the peri-implantation period in response to pregnancy status and heat stress.
A Grounded Theory Approach to Navigating Infertility Care During U.S. Military Service
Authors: Buechel Jennifer , Spalding Carmen N , Brock Whitney W , Dye Judy L , Todd Natalie , Wilson Candy , Fry-Bowers Eileen K 
Published: July 2022

ABSTRACT

Introduction
In this study, we aimed to understand how active duty service members and their partners navigate the infertility care process within the Military Health System (MHS) while managing a military career.

Materials and Methods
We obtained Institutional Review Board approval to employ a qualitative design using grounded theory methods. We recruited participants using purposive sampling, followed by theoretical sampling. We derived data from demographic questionnaires and semi-structured interviews. Consistent with grounded theory methods, we began analysis with line-by-line coding and moved to focused coding. We employed constant comparative analysis throughout the process to name, categorize, and conceptualize data and relationships.

Results
The participants included 28 patients, five partners, nine health care providers, and two military leaders. The infertility care process began with active duty service members and their partners recognizing the desire to have a child and discovering infertility, followed by deciding to seek infertility care. The experience was temporally bound within the context of the military environment. We identified the following themes, which described facilitators and barriers to accessing care: Duty station location, career stage, military versus the civilian cost of services, command climate, and policy. These facilitators and barriers varied widely across the Department of Defense (DoD), which resulted in fragmented and inconsistent care cycles, contributed to emotional and physical stress, and created tension between career progression and family formation.

Conclusions
Understanding how military couples perceive and manage demands of infertility care may enhance access to care, decrease patient costs, improve outcomes, result in better support for military couples who experience infertility, and ultimately improve the health and military readiness of our armed forces. The results support the need for action by providers, policy makers, and military leaders to develop effective infertility treatment programs and policies in the DoD.
Decades-long variations in NS-LMXBs observed with MAXI/GSC, RXTE/ASM, and Ginga/ASM
Authors: Asai Kazumi , Mihara Tatehiro , Matsuoka Masaru 
Published: July 2022

Abstract
We investigate the decades-long X-ray variations in bright low-mass X-ray binaries containing a neutron star (NS-LMXBs). The light curves of MAXI/GSC and RXTE/ASM cover ∼26 yr, and high-quality X-ray light curves are obtained from 33 NS-LMXBs. Among these, together with Ginga/ASM, two sources (GX 3+1 and GX 9+1) showed an apparent sinusoidal variation with respective periods of ∼5 and ∼10 yr in the 34 yr light curve. Their X-ray luminosities were (1–4) × 1037 erg s−1 in the middle of the NS-LMXB luminosity distribution. Seven other sources (Ser X-1, 4U 1735−444, GX 9+9, 4U 1746−37, 4U 1708−40, 4U 1822−000, and 1A 1246−588) have similar sinusoidal variations, although the profiles (amplitude, period, and phase) are variable. Compering the 21 sources with known orbital periods, one possible cause of the long-term sinusoidal variation might be mass-transfer cycles induced by irradiation to the donor star.
The plant ESCRT component FREE1 regulates peroxisome-mediated turnover of lipid droplets in germinating Arabidopsis seedlings
Authors: Huang Shuxian , Liu Zhiqi , Cao Wenhan , Li Hongbo , Zhang Wenxin , Cui Yong , Hu Shuai , Luo Mengqian , Zhu Ying , Zhao Qiong , Xie Lijuan , Gao Caiji , Xiao Shi , Jiang Liwen 
Published: July 2022

Abstract
Lipid droplets (LDs) stored during seed development are mobilized and provide essential energy and lipids to support seedling growth upon germination. Triacylglycerols (TAGs) are the main neutral lipids stored in LDs. The lipase SUGAR DEPENDENT 1 (SDP1), which hydrolyzes TAGs in Arabidopsis thaliana, is localized on peroxisomes and traffics to the LD surface through peroxisomal extension, but the underlying mechanism remains elusive. Here, we report a previously unknown function of a plant-unique endosomal sorting complex required for transport (ESCRT) component FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1) in regulating peroxisome/SDP1-mediated LD turnover in Arabidopsis. We showed that LD degradation was impaired in germinating free1 mutant; moreover, the tubulation of SDP1- or PEROXIN 11e (PEX11e)-marked peroxisomes and the migration of SDP1-positive peroxisomes to the LD surface were altered in the free1 mutant. Electron tomography analysis showed that peroxisomes failed to form tubules to engulf LDs in free1, unlike in the wild-type. FREE1 interacted directly with both PEX11e and SDP1, suggesting that these interactions may regulate peroxisomal extension and trafficking of the lipase SDP1 to LDs. Taken together, our results demonstrate a pivotal role for FREE1 in LD degradation in germinating seedlings via regulating peroxisomal tubulation and SDP1 targeting.
Signature of primordial non-Gaussianity on the 21 cm power spectrum from dark ages
Authors: Yamauchi Daisuke 
Published: July 2022

Abstract
We study the signature of primordial non-Gaussianity imprinted on the power spectrum of the 21 cm line differential brightness temperature during dark ages. Employing the perturbative treatment of gravitational clustering, we quantitatively estimate the effects of the non-Gaussian and one-loop corrections on the 21 cm power spectrum. The potential impact of the use of the 21 cm power spectrum for the constraint on local-type primordial non-Gaussianity is investigated based on the Fisher matrix analysis. Our results show that the 21 cm power spectrum for an array with a baseline of several tens of kilometers can constrain the primordial non-Gaussianity to a level severer than that from cosmic microwave background measurements, and its constraining power is stronger than that of the 21 cm bispectrum, while in the ultimate situation the 21 cm bispectrum eventually becomes more powerful.
TheĜ-Index of a Spin, Closed, Hyperbolic Manifold of Dimension 2 Or 4
Authors: Ratcliffe John G , Tschantz Steven T 
Published: July 2022

AbstractIn this paper, we develop general techniques for computing the Atiyah–Singer $\hat G$-index of a spin, closed, hyperbolic 2- or 4-manifold and apply these techniques to compute the $\hat G$-index of the fully symmetric spin structure of the Davis hyperbolic 4-manifold.
Recurrence rate of venous thromboembolic events in granulomatosis with polyangiitis
Authors: Nevares Alana , Yaseen Kinanah , Tamaki Hiromichi , Bena James , Messner William , Villa-Forte Alexandra 
Published: July 2022

Abstract

Objective
The incidence of first-time venous thromboembolic events (VTEs) is high in granulomatosis with polyangiitis (GPA). The incidence of recurrent VTEs is unknown. We aimed to describe the recurrence rate of second VTEs in patients with GPA.

Methods
Retrospective chart review was performed in patients with GPA and at least one VTE at a single centre from 2002 to 2016. Inclusion criteria were 1990 ACR criteria or 2012 Revised International Chapel Hill nomenclature for GPA, at least two follow-up visits, at least one VTE during the study period, and VTE occurrence after or within 3 months before GPA diagnosis. Second VTE event-free survival rates were estimated.

Results
Out of 147 patients initially screened for GPA and with at least one VTE, 84 met inclusion criteria. Median age at first VTE was 57 years. Incidence rate for second VTE was 8.4 events per 100 patient-years (95% CI: 5.7, 12.3). Eighty-three point three per cent of first VTEs and 57.7% of second VTEs occurred when disease was active (P &lt; 0.001). Renal involvement and constitutional symptoms at the time of first VTE were associated with VTE recurrence.

Conclusion
GPA has a high rate of VTE recurrence compared with the reported data in the general population with unprovoked VTE. Our results suggest that VTE in GPA is a recurrent co-morbidity, not always during active vasculitis, and more so in those with renal involvement and constitutional symptoms at the time of first VTE.
Cell osteogenic bioactivity mediated precisely by varying scaled micro-pits on ordered micro/nano hierarchical structures of titanium
Authors: Zhang Yanmei , Wang Xiankuan , Li Yaxian , Liang Jianhe , Jiang Pinliang , Huang Qiaoling , Yang Yun , Duan Hongping , Dong Xiang , Rui Gang , Lin Changjian 
Published: July 2022

Abstract
Hierarchical surface structures with micro–nano scale play a crucial role in regulation of cell proliferation and osteogenic differentiation. It has been proven that cells are extremely sensitive to the nanoscaled structure and show multifarious phenotypes. Though a vital function of microstructure on osseointegration has been confirmed, the cell performances response to different microscaled structure is needed to be further dissected and in depth understood. In this work, the ordered micro–nano hierarchical structures with varying micro-scaled pits were precisely fabricated on titanium successfully by the combination of electrochemical, chemical etching and anodization as well. In vitro systematical assessments indicated that the micro–nano multilevel structures on titanium exhibited excellent cells adhesion and spreading ability, as well as steerable proliferation and osteogenic differentiation behaviors. It is shown that smaller micro-pits and lower roughness of the hierarchical structures enabled faster cell propagation. Despite cell growth was delayed on micro–nano titanium with relatively larger cell-match-size micro-pits and roughness, osteogenic-specific genes were significantly elevated. Furthermore, the alkaline phosphatase activity, collagen secretion and extracellular matrix mineralization of MC3T3-E1 on multi-scaled titanium were suppressed by a large margin after adding IWP-2 (an inhibitor of Wnt/β-catenin signal pathway), indicating this pathway played a crucial part in cell osteogenic differentiation modulated by micro–nano structures.
Pathways to Refugee Protection for Women: Victims of Violence or Genuine Lesbians?
Authors: Dustin Moira 
Published: July 2022

Abstract
Failures in UK decision-making for women seeking protection from gender-based violence vary depending on the claimant’s (purported) sexuality. These failures are attributable, at least in part, to the application of the particular social group Refugee Convention ground which channels claims along two distinct pathways: one path, for women assumed to be straight, focuses on the violence that threatens them; in contrast, for lesbian and bisexual women, the focus is on their sexuality. In either case, the claimant’s autonomy and individuality is eclipsed, but different stereotypes come into play depending on her (imputed) sexuality. This article argues that greater use of the political opinion Convention ground, and a holistic, rights-based approach would improve refugee status determination for all women, regardless of their sexuality.
Effectiveness of an integrated primary care intervention in improving psychosocial outcomes among Latino adults with diabetes: the LUNA-D study
Authors: Castañeda Sheila F , Gallo Linda C , Garcia Melawhy L , Mendoza Paulina M , Gutierrez Angela P , Lopez-Gurrola Maria , Roesch Scott , Pichardo Margaret S , Muñoz Fatima , Talavera Gregory A 
Published: July 2022

Abstract

Objective
To compare the effectiveness of usual care (UC) versus a culturally tailored integrated care model in improving mental health symptoms for Latino patients with Type 2 diabetes mellitus (T2DM).

Methods
We conducted a two-arm randomized controlled trial from 2015 to 2019 at a federally qualified health center. Participants were 456 adults ages 23–80 years who had a previous diagnosis of T2DM and were not currently using insulin. Participants were randomly assigned to Integrated Care Intervention (ICI; including behavioral/mental healthcare, medical visits, health education and care coordination) or UC; standard of care including referrals for health education and behavioral/mental health care where appropriate. Intention-to-treat, multilevel models were used to compare group × time changes in depression and anxiety symptoms (PHQ-8; GAD-7) and perceived stress (PSS-10) across 6 months.

Results
Participant mean age was 55.7 years, 36.3% were male, and 63.7% were primarily Spanish speaking. Baseline sociodemographic factors and mental health symptoms across study arms were balanced. Significant group × time interaction effects were observed for anxiety and depression symptoms (p &lt; .05). Within the ICI and UC groups, mean depression symptom changes were −0.93 and −0.39 (p &lt; .01); anxiety symptom changes were −0.97 (p &lt; .01) and −0.11 (p = .35); and perceived stress changes were −1.56 and −1.27 (p &lt; .01), respectively.

Conclusions
Although both ICI and UC showed decreases over time, the ICI group evidenced larger, statistically significant changes in both depression and anxiety. Adapted integrated models of behavioral and chronic disease management appear to be effective and could be considered for usual care practices.

ClinicalTrials.gov Identifier
NCT03983499
Evidence-Based Considerations for the Design of an Open-Source Ventilator: A Systematic Review
Authors: Dave Chintan , Sivajohan Asaanth , Basmaji John , Slessarev Marat 
Published: July 2022

OBJECTIVE:
To inform the design of open-source ventilators, we performed a systematic review of clinical practice guidelines (CPGs) to consolidate the evidence on mechanical ventilation strategies that result in improved patient-important outcomes for acute hypoxic respiratory failure.

DATA SOURCES:
We developed a search strategy to identify relevant CPGs from Ovid Medline, Ovid Medline In-Process & Other Non-Indexed Citations, Embase, the Cochrane Library, Mendeley, and Google scholar from 2010 to February 17, 2022.

STUDY SELECTION:
Using a two-step screening process with two independent reviewers, we included CPGs that made recommendations on mechanical ventilation strategies of interest. Guidelines that reported at least one recommendation about mechanical ventilation in ICU patients with acute hypoxic respiratory failure were included.

DATA EXTRACTION:
From the 13 eligible guidelines, we collected data on country, aim, patient population, impact on morbidity and mortality (effect size and CIs), recommendations, strength of Recommendation (as per Grading of Recommendations, Assessment, Development and Evaluations), and details of supporting evidence base.

DATA SYNTHESIS:
We identified three ventilation strategies that confer a mortality and morbidity benefit for ventilated patients with acute hypoxic respiratory failure: low-tidal volume ventilation, plateau pressures of less than 30 cm H2O, and higher positive end-expiratory pressure (PEEP). These moderate-to-strong recommendations were based on moderate-to-high certainty in evidence. We identified several other recommendations with no or minimal certainty in evidence.

CONCLUSIONS:
Our systematic review of international CPGs identified no recommendations favoring specific mode of ventilation and three ventilation strategies that confer mortality and morbidity benefits, backed by moderate-to-strong evidence. Ventilator design teams must include the ability to consistently provide and measure low-tidal volume ventilation, plateau pressures of less than 30 cm H2O, and higher PEEP into their designs. Based on our findings, we provide the first public framework for open-source ventilator design.
Mystery Shopper Assessment of PrEP Service Delivery Quality for Adolescent Girls and Young Women in Kenya: A Cross-sectional Study
Authors: Kohler Pamela , Larsen Anna , Sila Joseph , Wilson Kate , Abuna Felix , Lagat Harison , Owiti George , Owens Tamara , Pintye Jillian , Richardson Barbra , John-Stewart Grace , Kinuthia John 
Published: July 2022

Abstract
Pre-exposure prophylaxis (PrEP) uptake and adherence among adolescent girls and young women (AGYW) may be negatively influenced by poor interactions with health care providers. We assessed PrEP counseling using unannounced standardized patient actors (USPs) at routine care clinics in Kenya. Trained actors posed as AGYW seeking PrEP services following case scripts and completed a checklist of provider adherence to national guidelines and communication skills. Scores were converted into a percentage and compared using linear regression. The overall mean quality score was 52.1 and varied across case scripts: a married new initiator yielded higher scores than portrayals of adherence/stigma challenges, transactional sex, and a minor adolescent. Mean guideline scores (31.4) were lower than communication scores (72.8), although in 36.5% of encounters, USPs stated they would not seek help from that provider again. Unannounced standardized patients reported provider reluctance to offer PrEP to AGYW. Interventions to strengthen provider counseling skills are needed.
Induction of antigenic immune tolerance to delay type 1 diabetes – challenges for clinical translation
Authors: Wesley Johnna D. , Pagni Philippe P. , Bergholdt Regine , Kreiner Frederik Flindt , von Herrath Matthias 
Published: July 2022

Purpose of review
Dissect the field of antigen-specific immunotherapy (ASIT) in type 1 diabetes (T1D), highlighting the major barriers currently blocking clinical translation.

Recent findings
ASIT remains a promising approach in T1D to re-establish the proper balance in the immune system to avoid the autoimmune-mediated attack or destruction of beta-cells in the pancreas. Despite some encouraging preclinical results, ASIT has not yet successfully translated into clinical utility, predominantly due to the lack of validated and clinically useful biomarkers.

Summary
To restore immune tolerance towards self-antigens, ASIT aims to establish a favourable balance between T effector cells and T regulatory cells. Whilst most ASITs, including systemic or oral administration of relevant antigens, have appeared safe in T1D, meaningful and durable preservation of functional beta-cell mass has not been proven clinically. Development, including clinical translation, remains negatively impacted by lack of predictive biomarkers with confirmed correlation between assay readout and clinical outcomes. To be able to address the high unmet medical need in T1D, we propose continued reinforced research to identify such biomarkers, as well efforts to ensure alignment in terms of trial design and conduct.
Determinants of healing and readiness for prosthetic fitting after transtibial amputation: Integrative literature review
Authors: Day Jonathan D. , Dionne Carol P. , James Shirley , Wang Hongwu 
Published: July 2022

Background:
Healing after an amputation is a critical step in the recovery process. Delayed wound healing can lead to deconditioning and flexion contractures which reduce a patient's ability to use a prosthesis, ambulate independently, and return to community participation. The purpose of this integrative literature review was to determine the evidence-based physiological factors, comorbidities, postoperative management, and outcome measures associated with healing after transtibial amputation and address how these factors clinically guide readiness for prosthetic intervention.

Methods:
Authors completed Google Scholar searches to identify the most effective search terms to locate salient publications. Authors also completed literature searches of Ovid MEDLINE and Epub Ahead of Print, In-Process, and Other Nonindexed Citations and Daily <1946 to August 6, 2020>; Embase Classic + Embase <1947 to August 6, 2020>; and CINAHL Complete <1946 to August 6, 2020> databases using the following search terms: “transtibial,” “trans-tibial,” “below knee,” “BKA,” “amputation,” amputation stump,” “amputee,” “wound healing,” and “heal/s/ed/ing.” The authors decided to include all levels of evidence to capture the maximum number of articles related to the determinants of healing and readiness for prosthetic fitting after transtibial amputation.

Results:
The searches identified 2067 potential articles for review, and after removing articles not relevant to the topic, authors completed full-text assessment on 20 articles. These included review and synthesis on three randomized controlled trials and 12 cohort studies.

Conclusion:
Preamputation assessment is most critical in patients who present with a longer list of comorbidities and suboptimal physiologic factors known to predict wound complications. Clinical judgment is most subjective when determining the degree of healing over time. Readiness for prosthetic treatment need not wait for complete healing of the residuum. Future research is needed to assess transcutaneous oxygen profusion along with other noninvasive measures of blood flow and perfusion as a more objective way to track progression of healing over time. This objective methodology would quantify healing, reduce subjectivity, and promote research to compare different enhanced recovery after surgery protocols for their impact on healing after amputation.