bluuebunny/crossref_metadata_2025_split huggingface dataset:
Future Population Exposure to Daytime and Nighttime Heat Waves in South Asia
Authors: Ullah Safi , You Qinglong , Chen Deliang , Sachindra D. A. , AghaKouchak Amir , Kang Shichang , Li Mingcai , Zhai Panmao , Ullah Waheed 
Published: April 2022

AbstractClimate change is expected to result in more frequent and intense heat waves (HWs) in South Asia (SA). The simultaneous increases in temperature and population will exacerbate the population exposure to future HWs. Here we estimate the future population exposure to daytime and nighttime HWs in SA using the Coupled Model Intercomparison Project 6 (CMIP6) models under four Shared Socioeconomic Pathways (SSPs) during 2061–2100, relative to 1975–2014. The results show that the projected frequency and spatial extent of the daytime (nighttime) HWs will be higher under scenario SSP5‐8.5, followed by SSP2‐4.5, SSP3‐7.0, and SSP1‐2.6 (SSP5‐8.5, followed by SSP3‐7.0, SSP2‐4.5, and SSP1‐2.6), relative to the historical period. The approach presented here allows decomposing the effects of climate change and future population on the overall exposure. The results reveal that the compounding effects of projected trends in population and HWs will significantly escalate the population exposure to HWs. Under the selected SSPs, the total population exposure to daytime and nighttime HWs ranges from 185 to 492 and 204–555 million people‐event, respectively, with the maximum exposure occurring in the Indo‐Gigantic Plain. The wide range of exposed populations highlights the sensitivity of the overall exposure to our future socioeconomic pathway decisions, emphasizing the importance of curbing anthropogenic greenhouse gas emissions and adopting sustainable urban planning solutions to minimize the potential socioeconomic and health impacts of HWs.
Evidence for Gravity Waves in the Thermosphere of Saturn and Implications for Global Circulation
Authors: Brown Zarah L. , Medvedev Alexander S. , Starichenko Ekaterina D. , Koskinen Tommi T. , Müller‐Wodarg Ingo C. F. 
Published: April 2022

AbstractGravity wave (GW) signatures have been derived from temperature profiles observed by Cassini/Ultraviolet Imaging Spectrograph in the Saturnian thermosphere during the Grand Finale campaign. They demonstrate upward propagation of GW packets, their saturation, and breaking. We determined wave amplitudes, potential energy, and momentum fluxes and estimated the associated wave drag imposed by dissipating harmonics on the ambient flow. The data set of 18 profiles covers the middle and high latitudes of both hemispheres, which allows for exploring the global impact of waves. The diagnostics based on the Transformed Eulerian Mean and modified geostrophy approach reveal that the GW drag induces an equatorward flow in both hemispheres, facilitating transport of heat away from the auroral zones and redistributing energy across latitudes. Like all the outer planets, Saturn's thermosphere is hundreds of degrees hotter than what follows from radiative balance and these results help to explain the observed temperatures at all latitudes.
Subduction Erosion Revealed by Late Mesozoic Magmatism in the Gangdese Arc, South Tibet
Authors: Huang Tong‐Yu , Wang Qiang , Wyman Derek A. , Ma Lin , Tang Gong‐Jian , Zhang Zhi‐Ping , Dong Han 
Published: April 2022

AbstractSubduction of oceanic lithosphere can cause crustal growth and destruction, but whether this balancing act is common in a single fossil convergent system has been unclear. Here we report geochronologic‐geochemical‐isotopic data on Mesozoic igneous rocks in the central‐eastern Gangdese arc. Our results reveal: (a) A coupling of magmatic arc migration toward the continent interior with a marked incompatible enrichment of sub‐arc mantle magma sources; (b) numerous Late Cretaceous adakites were generated via combined partial melting of both eroded forearc crust debris and subducted oceanic crust; (c) the truncation of forearc crust and extremely short arc‐suture distance. Our work demonstrates that although there was significant vertical growth of juvenile crust throughout the Gangdese arc during the Late Mesozoic, a large amount of continental crust was returned back into the mantle through lateral subduction erosion at the same time. This study provides important insights into long‐term material recycling on Earth.
A Model Intercomparison Analysis for Controls on C Accumulation in North American Peatlands
Authors: Zhao Bailu , Zhuang Qianlai , Treat Claire , Frolking Steve 
Published: April 2022

AbstractPeatland biogeochemical processes have not been adequately represented in existing earth system models, which might have biased the quantification of Arctic carbon‐climate feedbacks. We revise the Peatland Terrestrial Ecosystem Model (PTEM) by incorporating additional peatland biogeochemical processes. The revised PTEM is evaluated by comparing with Holocene Peatland Model (HPM) in simulating peat physical and biogeochemical dynamics in three North American peatlands: a permafrost‐free fen site, a permafrost‐free bog site and a permafrost bog site. Peatland carbon dynamics are simulated from peat initiation to 1990 and then to year 2300. Model responses to the changes in temperature and precipitation are analyzed to identify key processes affecting peatland carbon accumulation rates. We find that the net C balance is sensitive to water table depth and nutrient availability. Future simulations to year 2300 are conducted with both models under RCP 2.6, RCP 4.5, and RCP 8.5. PTEM predicts these peatlands to be C sources or weaker C sinks when insufficient precipitation suppresses soil moisture and thereby net N mineralization and net primary production, while HPM predicts the same when drier climate leads to increasing water table depth. Our results highlight the importance of water balance and C‐N feedback on peatland C dynamics. With a warmer climate, these peatlands could become a weaker C sink or a source under drier conditions, otherwise a larger C sink if wetter. Improved understanding to peatland processes can help future quantification of peatland C dynamics in the boreal and Arctic regions.
Complexity in the Evolution, Composition, and Spectroscopy of Brown Carbon in Aircraft Measurements of Wildfire Plumes
Authors: Washenfelder R. A. , Azzarello L. , Ball K. , Brown S. S. , Decker Z. C. J. , Franchin A. , Fredrickson C. D. , Hayden K. , Holmes C. D. , Middlebrook A. M. , Palm B. B. , Pierce R. B. , Price D. J. , Roberts J. M. , Robinson M. A. , Thornton J. A. , Womack C. C. , Young C. J. 
Published: April 2022

AbstractBiomass burning is a major source of light‐absorbing organic aerosol (brown carbon), but its composition, chemical evolution, and lifetime are not well known. We measured water‐soluble brown carbon absorption from 310 to 500 nm on the National Oceanic and Atmospheric Administration Twin Otter aircraft during flights downwind of western United States wildfires in summer 2019. The sampling strategy was near‐Lagrangian and the plume ages spanned 0–5 hr. Trends in brown carbon mass absorption coefficient with plume age varied between flights, and did not show an exponential decay over these short time scales. The measured absorption spectra were smoothly varying, without identifiable contributions from individual chromophores with structured absorption. Using aerosol tracer ions and reference absorption spectra, the calculated contribution of 4‐nitrocatechol to total absorption was <22 ± 9% and <11 ± 5%, although spectral fitting showed that it may be as low as <1.1% and <0.6% at 365 and 405 nm, respectively.
Dynamic synovial fibroblasts are modulated by NBCn1 as a potential target in rheumatoid arthritis
Authors: Ji Minjeong , Ryu Hee Jung , Baek Hyeon-Man , Shin Dong Min , Hong Jeong Hee 
Published: April 2022

AbstractRheumatoid arthritis (RA) is an autoimmune disease characterized by aggressive fibroblast-like synoviocytes (FLSs) and pannus formation. Various therapeutic strategies have been developed against inflammatory cytokines in RA in recent decades. Based on the migratory features of FLSs, we examined whether modulation of the migratory module attenuates RA severity. In this study, inflamed synovial fluid-stimulated FLSs exhibited enhanced migration and migratory apparatus expression, and sodium bicarbonate cotransporter n1 (NBCn1) was identified in primary cultured RA-FLSs for the first time. The NBC inhibitor S0859 attenuated the migration of FLSs induced with synovial fluid from patients with RA or with TNF-α stimulation. Inhibition of NBCs with S0859 in a collagen-induced arthritis (CIA) mouse model reduced joint swelling and destruction without blood, hepatic, or renal toxicity. Primary FLSs isolated from the CIA-induced mouse model also showed reduced migration in the presence of S0859. Our results suggest that inflammatory mediators in synovial fluid, including TNF-α, recruit NBCn1 to the plasma membrane of FLSs to provide dynamic properties and that modulation of NBCn1 could be developed into a therapeutic strategy for RA.
Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
Authors: Georgievski Aleksandra , Michel Anaïs , Thomas Charles , Mlamla Zandile , Pais de Barros Jean-Paul , Lemaire-Ewing Stéphanie , Garrido Carmen , Quéré Ronan 
Published: April 2022

AbstractPatient-derived xenografted (PDX) models were generated through the transplantation of primary acute lymphoblastic leukemia (ALL) cells into immunodeficient NSG mice. We observed that ALL cells from mouse bone marrow (BM) produced extracellular vesicles (EVs) with specific expression of inducible heat shock protein HSP70, which is commonly activated in cancer cells. Taking advantage of this specific expression, we designed a strategy to generate fluorescent HSP70-labeled ALL EVs and monitor the impact of these EVs on endogenous murine BM cells ex vivo and in vivo. We discovered that hematopoietic stem and progenitor cells (HSPC) were mainly targeted by ALL EVs, affecting their quiescence and maintenance in the murine BM environment. Investigations revealed that ALL EVs were enriched in cholesterol and other metabolites that contribute to promote the mitochondrial function in targeted HSPC. Furthermore, using CD34+ cells isolated from cord blood, we confirmed that ALL EVs can modify quiescence of human HSPC. In conclusion, we have discovered a new oncogenic mechanism illustrating how EVs produced by proliferative ALL cells can target and compromise a healthy hematopoiesis system during leukemia development.
The brainstem connectome database
Authors: Schmitt Oliver , Eipert Peter , Ruß Frauke , Beier Julia , Kadir Kanar , Horn Anja 
Published: April 2022

AbstractConnectivity data of the nervous system and subdivisions, such as the brainstem, cerebral cortex and subcortical nuclei, are necessary to understand connectional structures, predict effects of connectional disorders and simulate network dynamics. For that purpose, a database was built and analyzed which comprises all known directed and weighted connections within the rat brainstem. A longterm metastudy of original research publications describing tract tracing results form the foundation of the brainstem connectome (BC) database which can be analyzed directly in the framework neuroVIISAS. The BC database can be accessed directly by connectivity tables, a web-based tool and the framework. Analysis of global and local network properties, a motif analysis, and a community analysis of the brainstem connectome provides insight into its network organization. For example, we found that BC is a scale-free network with a small-world connectivity. The Louvain modularity and weighted stochastic block matching resulted in partially matching of functions and connectivity. BC modeling was performed to demonstrate signal propagation through the somatosensory pathway which is affected in Multiple sclerosis.
A multi-million image Serial Femtosecond Crystallography dataset collected at the European XFEL
Authors: Kirkwood Henry J. , de Wijn Raphael , Mills Grant , Letrun Romain , Kloos Marco , Vakili Mohammad , Karnevskiy Mikhail , Ahmed Karim , Bean Richard J. , Bielecki Johan , Dall’Antonia Fabio , Kim Yoonhee , Kim Chan , Koliyadu Jayanath , Round Adam , Sato Tokushi , Sikorski Marcin , Vagovič Patrik , Sztuk-Dambietz Jolanta , Mancuso Adrian P. 
Published: April 2022

AbstractSerial femtosecond crystallography is a rapidly developing method for determining the structure of biomolecules for samples which have proven challenging with conventional X-ray crystallography, such as for membrane proteins and microcrystals, or for time-resolved studies. The European XFEL, the first high repetition rate hard X-ray free electron laser, provides the ability to record diffraction data at more than an order of magnitude faster than previously achievable, putting increased demand on sample delivery and data processing. This work describes a publicly available serial femtosecond crystallography dataset collected at the SPB/SFX instrument at the European XFEL. This dataset contains information suitable for algorithmic development for detector calibration, image classification and structure determination, as well as testing and training for future users of the European XFEL and other XFELs.
Parallel gold enhancement of quantum dots 565/655 for double-labelling correlative light and electron microscopy on human autopsied samples
Authors: Uematsu Miho , Mikami Kyohei , Nakamura Ayako , Takahashi Ryosuke , Yokota Takanori , Hirokawa Katsuiku , Uchihara Toshiki 
Published: April 2022

AbstractCadmium selenide quantum dots (QDs) are fluorescent and electron-dense nanoparticles. When used as reporter of immunolabeling, this dual visibility is essential for direct comparison of its fluorescent signals on light microscopy (LM) and their ultrastructrual counterparts on electron microscopy (EM) as correlative light and electron microscopy (CLEM). To facilitate EM recognition, QDs on EM grid were gold enhanced, which increased their size and electron density. On histological sections as well, gold-enhanced QDs, used as a reporter of immunolabeling, were easily recognized on EM. Because target structures are visible on bright field microscopy, gold enhancement facilitated trimming the target structures into final EM sections. Furthermore, gold enhancement of rod-shaped QD655 on EM grid was accentuated on their tips while spherical QD565 was gold-enhanced as sphere in contrast. This EM distinction was evident on histological sections where QD565 (green fluorescence) and QD655 (red fluorescence) were used as a reporter pair for double immunolabeling. Double-labeled immuno-fluorescent images, initially captured before EM processing, are now compared with their respective immuno EM counterparts. Specific labeling of each epitope was corroborated by mutual comparison between LM and EM. Although fluoronanogold may be a candidate reporter partner with QDs for gold-enhanced, double-labeling CLEM, its limited penetration into fixed tissue hampers universal use for thick histological sections. Gold-enhancement of QD immunolabeling, now expanded to double-labeling CLEM for human brain samples, will pave the way to translate molecular events into ultrastructural morphopathogenesis in situ.
High genomic diversity in the endangered East Greenland Svalbard Barents Sea stock of bowhead whales (Balaena mysticetus)
Authors: Cerca José , Westbury Michael V. , Heide-Jørgensen Mads Peter , Kovacs Kit M. , Lorenzen Eline D. , Lydersen Christian , Shpak Olga V. , Wiig Øystein , Bachmann Lutz 
Published: April 2022

AbstractThe East Greenland-Svalbard-Barents Sea (EGSB) bowhead whale stock (Balaena mysticetus) was hunted to near extinction and remainsEndangeredon the International Union of Conservation of Nature Red List. The intense, temporally extensive hunting pressure may have left the population vulnerable to other perturbations, such as environmental change. However, the lack of genomic baseline data renders it difficult to evaluate the impacts of various potential stressors on this stock. Twelve EGSB bowhead whales sampled in 2017/2018 were re-sequenced and mapped to a previously published draft genome. All individuals were unrelated and void of significant signs of inbreeding, with similar observed and expected homo- and heterozygosity levels. Despite the small population size, mean autosome-wide heterozygosity was 0.00102, which is higher than that of most mammals for which comparable estimates are calculated using the same parameters, and three times higher than a conspecific individual from the Eastern-Canada-West-Greenland bowhead whale stock. Demographic history analyses indicated a continual decrease ofNefrom ca. 1.5 million to ca. 250,000 years ago, followed by a slight increase until ca. 100,000 years ago, followed by a rapid decrease inNebetween 50,000 and 10,000 years ago. These estimates are lower than previously suggested based on mitochondrial DNA, but suggested demographic patterns over time are similar.
Acute and long-term effects of cannabinoids on hypertension and kidney injury
Authors: Golosova Daria , Levchenko Vladislav , Kravtsova Olha , Palygin Oleg , Staruschenko Alexander 
Published: April 2022

AbstractCannabinoids and their endogenous and synthetic analogs impact blood pressure and contribute to the incidence of hypertension. It was previously reported that the endocannabinoid system plays an important role in developing hypertension; however, it was also shown that cannabinoids elicit profound hypotension associated with hemorrhagic, cardiogenic, and endotoxic shock. This study aimed to test acute and chronic effects of an endogenous ligand of cannabinoid receptor anandamide (AEA) on blood pressure and kidney injury in vivo in conscious Dahl salt-sensitive (SS) rats. We demonstrated that acute i.v. bolus administration of a low or a high doses (0.05 or 3 mg/kg) of AEA did not affect blood pressure for 2 h after the injection in Dahl SS rats fed a normal salt diet (0.4% NaCl). Neither low nor high doses of AEA had any beneficial effects on blood pressure or kidney function. Furthermore, hypertensive rats fed a HS diet (8% NaCl) and chronically treated with 3 mg/kg of AEA exhibited a significant increase in blood pressure accompanied by increased renal interstitial fibrosis and glomerular damage at the late stage of hypertension. Western blot analyses revealed increased expression of Smad3 protein levels in the kidney cortex in response to chronic treatment with a high AEA dose. Therefore, TGF-β1/Smad3 signaling pathway may play a crucial role in kidney injury in SS hypertension during chronic treatment with AEA. Collectively, these data indicate that prolonged stimulation of cannabinoid receptors may result in aggravation of hypertension and kidney damage.
Large-scale forecasting of Heracleum sosnowskyi habitat suitability under the climate change on publicly available data
Authors: Koldasbayeva Diana , Tregubova Polina , Shadrin Dmitrii , Gasanov Mikhail , Pukalchik Maria 
Published: April 2022

AbstractThis research aims to establish the possible habitat suitability of Heracleum sosnowskyi (HS), one of the most aggressive invasive plants, in current and future climate conditions across the territory of the European part of Russia. We utilised a species distribution modelling framework using publicly available data of plant occurrence collected in citizen science projects (CSP). Climatic variables and soil characteristics were considered to follow possible dependencies with environmental factors. We applied Random Forest to classify the study area. We addressed the problem of sampling bias in CSP data by optimising the sampling size and implementing a spatial cross-validation scheme. According to the Random Forest model built on the finally selected data shape, more than half of the studied territory in the current climate corresponds to a suitability prediction score higher than 0.25. The forecast of habitat suitability in future climate was highly similar for all climate models. Almost the whole studied territory showed the possibility for spread with an average suitability score of 0.4. The mean temperature of the wettest quarter and precipitation of wettest month demonstrated the highest influence on the HS distribution. Thus, currently, the whole study area, excluding the north, may be considered as s territory with a high risk of HS spreading, while in the future suitable locations for the HS habitat will include high latitudes. We showed that chosen geodata pre-processing, and cross-validation based on geospatial blocks reduced significantly the sampling bias. Obtained predictions could help to assess the risks accompanying the studied plant invasion capturing the patterns of the spread, and can be used for the conservation actions planning.
Investigating functional fitness of older adults in Korea in the period 2013–2018
Authors: Jeoung Bogja , Pyun Do Young 
Published: April 2022

AbstractThe purpose of this study is two-fold: (a) to examine differences in the body compositions and the functional fitness tests across genders, age groups, and the test periods; and (b) to develop the functional fitness standards for older Korean adults. This is a cross-sectional study using data from the Korea Institute of Sport Science Fitness Standards. The data were derived from 155,256 old adults aged 65–90 who participated in the National Fitness Award Project from 2013 to 2018 in Korea. A series of t-test, ANOVA, and Cajori’s 5-grade method were used to analyze the data. The group comparison tests revealed significant differences in the body compositions and the fitness tests across genders (p < 0.001 for all items), five age groups (p < 0.001 for all items), and two test periods (p < 0.001 for all items, except for sit-reach [p = 0.138] in males and body mass index [p = 0.258] and grip strength [p = 0.130] in females). The study also proposed the guidelines for the functional fitness standards for this population. This study would provide useful information to practitioners to design more effective physical activity programs or interventions for people at-risk of declining health, thus improving health conditions of the older adults in Korea.
Reflectance and photophysical properties of rhodamine 6G/2-(4-methyl-2-oxo-2H-chromen-7-yloxy) acetic acid as cold hybrid colorant
Authors: Gheitarani Behnam , Golshan Marzieh , Hosseini Mahdi Salami , Salami-Kalajahi Mehdi 
Published: April 2022

AbstractRhodamine 6G (Rh6G) is modified by ethylenediamine to obtain rhodamine with amine functional groups (Rh6G-NH2). Rh6G-NH2 as an initial core is used to bond coumarin derivatives. Synthesized fluorescent colorants are specified using Fourier transform infrared spectroscopy (FT-IR), proton and carbon nuclear magnetic resonance (1H NMR and 13C NMR), X-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM) to analyze the structure of the fluorescent pigments. Fluorescence microscopy, fluorescence spectrophotometer, and UV–visible–NIR reflectance spectra are used to demonstrate the optical properties. UV–Vis–NIR reflectance spectra showed that synthesized colorants were transparent in NIR region. Also, photophysical properties of 2-(4-methyl-2-oxo-2H-chromen-7-yloxy) acetic acid (MOHCYAA), Rh6G-NH2, and hybrid 2-(4-methyl-2-oxo-2H-chromen-7-yloxy) acetic acid/rhodamine 6G (HMR) were investigated. Type of solvent had a strong effect on quantum yield. Rh6G-NH2 (ϕs = 0.66) and HMR (ϕs = 0.72) displayed the maximum quantum yield in ethanol due to good interaction with ethanol and the formation of ring-opened amide form of rhodamine group. Finally, Rh6G-NH2 and HMR displayed the maximum quantum yield in ethanol due to good interaction of structure with ethanol and the formation of ring-opened amide form of rhodamine group in compound.