bluuebunny/crossref_metadata_2025_split huggingface dataset:
H and HL synergistically regulate jasmonate-triggered trichome formation in tomato
Authors: Hua Bing , Chang Jiang , Han Xiaoqian , Xu Zhijing , Hu Shourong , Li Shuang , Wang Renyin , Yang Liling , Yang Meina , Wu Shasha , Shen Jingyuan , Yu Xiaomin , Wu Shuang 
Published: January 2022

Abstract
The development of trichomes, which protect plants against herbivores, is affected by various stresses. In tomato, previous studies showed that stress-triggered jasmonic acid (JA) signaling influences trichome formation, but the underlying mechanism is not fully resolved. Here, we found that two C2H2 zinc finger proteins synergistically regulate JA-induced trichome formation in tomato. The naturally occurring mutations in the H gene and its close homolog H-like in a spontaneous mutant, LA3172, cause severely affected trichome development. Compared with the respective single mutant, the h/hl double mutant displayed more severe trichome defects in all tissues. Despite their partially redundant function, the H and HL genes regulate trichome formation in a spatially distinct manner, with HL more involved in hypocotyls and leaves while H is more involved in stems and sepals. Furthermore, the activity of H/HL is essential for JA-triggered trichome formation. The JA signaling inhibitor SlJAZ2 represses the activity of H and HL via physical interaction, resulting in the activation of THM1, a negative regulator of trichome formation. Our results provide novel insight into the mechanism of trichome formation in response to stress-induced JA signaling in tomato.
Getting to the Root of US Healthcare Injustices through Morrison’s Root Workers
Authors: Anatol Giselle Liza 
Published: January 2022

Abstract
Although a number of scholars have tackled the figure of the Black folk-healer in Toni Morrison’s novels, the character deserves greater attention in the present moment for the insights she provides into two contemporary catastrophes: the coronavirus pandemic and the structural racism that precipitates rampant violence against brown-skinned people in the United States. Beginning with M’Dear, the elderly woman who is brought in to treat Cholly’s Aunt Jimmy in The Bluest Eye (1970), I survey descriptions of several root workers, hoodoo practitioners, and midwives in Morrison’s fiction, including Ajax’s mother in Sula (1973) and Milkman’s aunt Pilate in Song of Solomon (1977). Morrison’s portraits of these women and their communities capture the endurance of African folk customs, the undervalued knowledge of aged members of society, and a sense of Black women’s strength beyond that of the physical, laboring, or hypersexual body. The fictional experiences of Morrison’s healers also alert readers to the very real injustices that have historically impeded the successes of African Americans—and continue to hamper them, as has been exposed during the COVID-19 crisis and public outrages over police brutality. These injustices include inequities in lifelong earning potential, education, housing, and access to healthcare. Paying closer attention to the Nobel Laureate’s root-working women makes her novels more than simply “transformative” and “empowering” for individual readers; analyzing these figures allows one to unearth important critiques of medical bias and other forms of discrimination against marginalized members of society—disparities that must be dismantled in the push for social change.
Public Support for Cigarette Pack Pictorial Health Warnings Among US Adults: A Cross-sectional Analysis of the 2020 Health Information National Trends Survey
Authors: Kaufman Annette R , D’Angelo Heather , Gaysynsky Anna , Seidenberg Andrew B , Vollinger Robert E , Blake Kelly D 
Published: January 2022

Abstract

Introduction
The US Food and Drug Administration issued a final rule requiring new warnings for cigarette packages and advertisements. This study examines population-level characteristics of support for—versus neutrality or opposition toward—cigarette pack warnings that use text and images to portray the negative health effects of smoking.

Methods
We used nationally representative cross-sectional data of US adults age 18 and older from the 2020 Health Information National Trends Survey (n = 3865). Frequencies and weighted proportions were calculated for neutrality toward, opposition to, and support for pictorial warnings across sociodemographics and other predictors. Weighted, multivariable logistic regression examined predictors of being neutral or opposed versus supportive of pictorial warnings.

Results
In 2020, an estimated 69.9% of US adults supported pictorial warnings, 9.1% opposed, and 20.9% neither supported nor opposed them. In fully adjusted models, current smokers had almost twice the odds of being neutral or opposed to pictorial warnings as never smokers (odds ratio [OR] = 1.99, confidence interval [CI] 1.12, 3.52). Adults 75 years and older (vs. 18–34) (OR = 0.55, CI 0.33, 0.94) and those with children under 18 in their household (vs. no children) (OR = 0.67, CI 0.46, 0.98) were less likely to be neutral or opposed.

Conclusions
In advance of the Food and Drug Administration’s implementation of pictorial warnings on cigarette packages, nearly 70% of American adults support this policy. Disseminating information about the effectiveness of pictorial warnings may further strengthen support among current smokers who are less supportive than never smokers. Furthermore, framing messages around the benefits of pictorial warnings for protecting youth may increase public support.

Implications
While public support for pictorial warnings on cigarette packages is high in the United States, it may increase further after policy implementation and be strengthened by utilizing information campaigns that convey the evidence that pictorial warnings are an effective public health strategy.
“New normal” at work in a post-COVID world: work–life balance and labor markets
Authors: Vyas Lina 
Published: January 2022

Abstract
The coronavirus pandemic has interrupted labor markets, triggering massive and instant series of experimentations with flexible work arrangements, and new relationships to centralized working environments. These approaches have laid the basis for the “new normal,” likely extending into the organization of work in the post-pandemic era. These new arrangements, especially flexible work arrangements, have challenged traditional relationships with employees and employers, work time and working hours, the work–life balance (WLB), and the relationship of individuals to work. This paper investigates how labor markets have been interrupted due to the pandemic, focusing especially on manual (blue-collar) and nonmanual (white-collar) work and the future of the WLB, along with exploring the projected deviations that are driving a foreseeable future policy revolution in work and employment. This paper argues that although hybrid and remote working would be more popular in the post-pandemic for nonmanual work, it will not be “one size fits all” solution. Traditional work practices will remain, and offices will not completely disappear. Manual labor will continue current work practices with increased demands. Employers’ attention to employees’ WLB in the new normal will target employees’ motivation and achieving better WLB. These trends for the labor market and WLB are classified into three categories—those that are predicated on changes that were already underway but were accelerated with arrival of the pandemic (“acceleration”); those that represent normalization of what were once considered avant-garde ways of work (“normalization”); and those that represent modification or alteration of pre-pandemic set-up (“remodelling”).
Monocyclic Components and Photosynthetic Damage Caused by Myrtle Rust in Guava Leaves
Authors: Gonçalves Manoel Penachio , Nogueira Júnior Antonio Fernandes , Ribeiro Rafael Vasconcelos , Amorim Lilian 
Published: January 2022

Austropuccinia psidii, the causal agent of myrtle rust, was, for many years, restricted to the Americas, but since reaching Hawaii in 2005, the pathogen has expanded its global range exponentially. In Brazil, myrtle rust is the main fungal disease in guava plants. Despite this, there are few studies on guava rust epidemiology. The objectives of this study were to quantify the monocyclic components of rust and to evaluate the photosynthetic damage caused by A. psidii in young and old leaves of ‘Paluma’ guava. The monocyclic components of guava rust and gas exchange in healthy or inoculated (105 ml–1 urediniospores of A. psidii) leaves were quantified over time. Additionally, young leaves were inoculated with varying concentrations of A. psidii inoculum, and leaf gas exchange and chlorophyll fluorescence were measured at 25 days postinoculation. The relationship between the relative CO2 assimilation of a diseased leaf (Px) and a healthy leaf (Po) is related to disease severity (x) by Px/Po = (1 – x)β. The density of lesions, disease severity, and urediniospore production were high in young leaves, averaging 58 lesions cm–2, 50% leaf area diseased, and 2.5 × 104 urediniospores per lesion, respectively. Rust symptoms were not observed in old leaves, and resistance to infection did not cause any photosynthetic cost to these leaves. On young leaves, β was 2.13, indicating a reduction on CO2 assimilation at green tissues from symptomatic leaves. Our data revealed that photosynthesis reduction in diseased guava leaves was caused by biochemical and photochemical damage rather than by stomatal limitation.
Exposure to 1800 MHz LTE electromagnetic fields under proinflammatory conditions decreases the response strength and increases the acoustic threshold of auditory cortical neurons
Authors: Souffi Samira , Lameth Julie , Gaucher Quentin , Arnaud-Cormos Délia , Lévêque Philippe , Edeline Jean-Marc , Mallat Michel 
Published: January 2022

AbstractIncreased needs in mobile phone communications have raised successive generations (G) of wireless technologies, which could differentially affect biological systems. We have assessed how a single 2h head-only exposure to a 4G long term evolution (LTE)-1800 MHz electromagnetic field (EMF) impacts on the microglial space coverage and the electrophysiological neuronal activity in the primary auditory cortex (ACx) in rats submitted to an acute neuroinflammation induced by lipopolysaccharide. The mean specific absorption rate in the ACx was 0.5 W/kg. Multiunit recording revealed that LTE-EMF triggered reduction in the response strength to pure tones and to natural vocalizations, together with an increase in acoustic threshold in the low and medium frequencies. Iba1 immunohistochemistry showed no change in the area covered by microglia cell bodies and processes. In healthy rats, the same LTE-exposure induced no change in response strength and acoustic threshold. Our data indicate that an acute neuroinflammation sensitize neuronal responses to LTE-EMF, which leads to an altered processing of acoustic stimuli in the ACx.
ELF5 is a respiratory epithelial cell-specific risk gene for severe COVID-19
Authors: Pietzner Maik , Chua Robert Lorenz , Wheeler Eleanor , Jechow Katharina , Radbruch Helena , Trump Saskia , Heidecker Bettina , Heppner Frank L. , Eils Roland , Mall Marcus A. , Sander Leif-Erik , Lehmann Irina , Lukassen Sören , Wareham Nick , Conrad Christian , Langenberg Claudia 
Published: January 2022

ABSTRACTDespite two years of intense global research activity, host genetic factors that predispose to a poorer prognosis and severe course of COVID-19 infection remain poorly understood. Here, we identified eight candidate protein mediators of COVID-19 outcomes by establishing a shared genetic architecture at protein-coding loci using large-scale human genetic studies. The transcription factor ELF5 (ELF5) showed robust and directionally consistent associations across different outcome definitions, including a >4-fold higher risk (odds ratio: 4.85; 95%-CI: 2.65-8.89; p-value<3.1×10−7) for severe COVID-19 per 1 s.d. higher genetically predicted plasma ELF5. We show that ELF5 is specifically expressed in epithelial cells of the respiratory system, such as secretory and alveolar type 2 cells, using single-cell RNA sequencing and immunohistochemistry. These cells are also likely targets of SARS-CoV-2 by colocalisation with key host factors, including ACE2 and TMPRSS2. We also observed a 25% reduced risk of severe COVID-19 per 1 s.d. higher genetically predicted plasma G-CSF, a finding corroborated by a clinical trial of recombinant human G-CSF in COVID-19 patients with lymphopenia reporting a lower number of patients developing critical illness and death. In summary, large-scale human genetic studies together with gene expression at single-cell resolution highlight ELF5 as a novel risk gene for COVID-19 prognosis, supporting a role of epithelial cells of the respiratory system in the adverse host response to SARS-CoV-2.
Robust, long-term video EEG monitoring in a porcine model of post-traumatic epilepsy
Authors: Martinez-Ramirez Luis , Slate Andrea , Price George , Duhaime Ann-Christine , Staley Kevin , Costine-Bartell Beth A. 
Published: January 2022

AbstractTo date, post-traumatic epilepsy (PTE) research in large animal models has been limited. Recent advances in neocortical microscopy have made possible new insights into neocortical PTE. However, it is very difficult to engender convincing neocortical PTE in rodents. Thus, large animal models that develop neocortical PTE may provide useful insights that also can be more comparable to human patients. Because gyrencephalic species have prolonged latent periods, long-term video EEG recording is required. Here, we report a fully subcutaneous EEG implant with synchronized video in freely ambulatory swine for up to 14 months during epileptogenesis following bilateral cortical impact injuries or sham surgery The advantages of this system include the availability of a commercially available system that is simple to install, a low failure rate after surgery for EEG implantation, radiotelemetry that enables continuous monitoring of freely ambulating animals, excellent synchronization to video to EEG, and a robust signal to noise ratio. The disadvantages of this system in this species and age are the accretion of skull bone which entirely embedded a subset of skull screws and EEG electrodes, and the inability to rearrange the EEG electrode array. These disadvantages may be overcome by splicing a subdural electrode strip to the electrode leads so that skull growth is less likely to interfere with long-term signal capture and by placing two implants for a more extensive montage. This commercially available system in this bilateral cortical impact swine model may be useful to a wide range of investigators studying epileptogenesis in PTE.SignificancePost-traumatic epilepsy (PTE) is a cause of significant morbidity after traumatic brain injury (TBI) and is often drug-resistant. Robust, informative animal models would greatly facilitate PTE research. Ideally, this biofidelic model of PTE would utilize a species that approximates human brain anatomy, brain size, glial populations, and inflammatory pathways. An ideal model would also incorporate feasible methods for long-term video EEG recording required to quantify seizure activity. Here, we describe the first model of PTE in swine and describe a method for robust long-term video EEG monitoring for up to 14 months post-TBI. The relatively easy “out-of-the-box” radiotelemetry system and surgical techniques described here will be adaptable by a wide array of investigators studying the pathogenesis and treatment of PTE.
Combiroc: when ‘less is more’ in bulk and single cell marker signatures
Authors: Ferrari I. , Mazzara S. , Crosti M. , Abrignani S. , Grifantini R. , Bombaci M. , Rossi R.L. 
Published: January 2022

AbstractHere we present thecombirocR package, for signatures refinement in high throughput omics. Based on a ROC-driven marker selection, it can be used to find powerful smaller sub-signatures from scRNAseq experiments and to annotate cells using fewer markers. Trained on PBMC dataset, combiroc found NK marker combinations with high cell-discriminating power, in agreement with human protein atlas and that were validated both computationally and experimentally on independent datasets.
Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse Striatum
Authors: Carpenter Marco D. , Fischer Delaney K. , Zhang Shuo , Bond Allison M , Czarnecki Kyle S. , Woolf Morgan T. , Song Hongjun , Heller Elizabeth A. 
Published: January 2022

AbstractHistone post-translational modifications (hPTMs) regulate gene expression via changes in chromatin accessibility and transcription factor recruitment. At a given locus, the coordinated enrichment of several distinct hPTMs regulate gene expression in response to external stimuli. However, neuronal hPTMs have been primarily characterized in bulk brain tissue and/or tissue pooled across subjects. This obscures both cell-type and individual variability, features essential to understand individual susceptibility to psychiatric disease. To address this limitation, we optimized a hybrid protocol, ICuRuS, to profile both activating and repressive hPTMs in neuronal subtypes from a single mouse. We report here profiling of striatal medium spiny neuron (MSN) subtypes, genetically defined by expression of Adenosine 2a Receptor (A2a) or Dopamine Receptor D1 (D1), which differentially regulate reward processing and pathophysiology. Using ICuRuS, we defined genome-wide, A2a- or D1-specific combinatorial hPTM profiles, and discovered regulatory epigenomic features at genes implicated in neurobiological function and disease.
Espalier: Efficient tree reconciliation and ARG reconstruction using maximum agreement forests
Authors: Rasmussen David A. , Guo Fangfang 
Published: January 2022

AbstractIn the presence of recombination individuals may inherit different regions of their genome from different ancestors, resulting in a mosaic of phylogenetic histories across their genome. Ancestral recombination graphs (ARGs) can capture how phylogenetic relationships vary across the genome due to recombination, but reconstructing ARGs from genomic sequence data is notoriously difficult. Here we present a method for reconciling discordant phylogenetic trees and reconstructing ARGs using maximum agreement forests (MAFs). Given two discordant trees, a MAF identifies a set of topologically concordant subtrees present in both trees. We show how discordant trees can be reconciled through their MAF in a way that retains discordances strongly supported by sequence data while eliminating conflicts likely attributable to phylogenetic noise. We further show how MAFs and our reconciliation approach can be combined to select a path of local trees across the genome that maximizes the likelihood of the genomic sequence data, minimizes discordance between neighboring local trees, and identifies the recombination events necessary to explain remaining discordances to obtain a fully connected ARG. While heuristic, our ARG reconstruction approach is often as accurate as more exact methods while being much more computationally efficient. Moreover, important demographic parameters such as recombination rates can be accurately estimated from reconstructed ARGs. Finally, we apply our approach to plant infecting RNA viruses in the genusPotyvirusto demonstrate how true recombination events can be disentangled from phylogenetic noise using our ARG reconstruction methods.
Xeno-free induced pluripotent stem cell-derived neural progenitor cells for in vivo applications
Authors: Rust Ruslan , Weber Rebecca Z. , Generali Melanie , Kehl Debora , Bodenmann Chantal , Uhr Daniela , Wanner Debora , Zürcher Kathrin J. , Saito Hirohide , Hoerstrup Simon P. , Nitsch Roger M. , Tackenberg Christian 
Published: January 2022

AbstractCell-based therapies are a promising treatment paradigm for neurodegenerative diseases and other brain injuries. Despite recent advances in stem cell technology, major concerns have been raised regarding the feasibility and safety of cell therapies for clinical applications. Here, we generate good manufacturing practice (GMP)-compatible neural progenitor cells (NPCs) from transgene- and xeno-free induced pluripotent stem cells (iPSCs) that can be smoothly adapted for clinical applications. The produced NPCs have a stable gene-expression over at least 15 passages and can be scaled for up to 1018 cells per initially seeded 106 cells. To ensure a pure NPC population for in vivo applications, we reduce risks of iPSC contamination using micro RNA-switch technology as a safety checkpoint. Using lentiviral transduction with a fluorescent and bioluminescent dual-reporter construct, combined with non-invasive in vivo bioluminescent imaging, we longitudinally tracked the grafted cells in healthy wild-type and genetically immunosuppressed mice as well as in a mouse model of ischemic stroke. Long term in-depth characterization revealed that transplanted cells have the capability to survive and spontaneously differentiate into functional and mature neurons throughout a time course of a month.
Connecting the dots: Harnessing dual-site transcranial magnetic stimulation to assess the causal influence of medial frontal areas on the motor cortex
Authors: Neige Cécilia , Vassiliadis Pierre , Zazou Abdelkrim Ali , Dricot Laurence , Lebon Florent , Brees Thomas , Derosiere Gerard 
Published: January 2022

AbstractDual-site transcranial magnetic stimulation (TMS) has been widely employed to investigate the influence of cortical structures on the primary motor cortex (M1). Here, we leveraged this technique to probe the causal influence of two key areas of the medial frontal cortex, namely the supplementary motor area (SMA) and the medial orbitofrontal cortex (mOFC), on M1. We show that SMA stimulation facilitates M1 activity across short (6 and 8 ms) and long (12 ms) inter-stimulation intervals, putatively recruiting cortico-cortical and cortico-subcortico-cortical circuits, respectively. Crucially, magnetic resonance imaging revealed that this facilitatory effect depended on a key morphometric feature of SMA: individuals with larger SMA volumes exhibited more facilitation from SMA to M1. Notably, we also provide evidence that the facilitatory effect of SMA stimulation at short intervals did not arise from spinal interactions of volleys descending simultaneously from SMA and M1. On the other hand, mOFC stimulation moderately suppressed M1 activity at both short and long intervals, irrespective of mOFC volume. These results suggest that dual-site TMS is an interesting tool to study the differential influence of SMA and mOFC on M1 activity, paving the way for the multi-modal assessment of these fronto-motor circuits in health and disease.Key pointsDual-site TMS has been widely employed to investigate effective connectivity between cortical structures and the primary motor cortex (M1).Here, we probed the causal influence of the supplementary motor area (SMA) and the medial orbitofrontal cortex (mOFC) on M1 activity.SMA stimulation facilitates M1 activity at both short and long inter-stimulation intervals; this facilitatory effect is related to SMA volume.mOFC stimulation moderately suppresses M1 activity, independent of mOFC volume.The findings pave the way for multi-modal assessment of fronto-motor circuits in health and disease.