bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gut microbiota from Mexican patients with metabolic syndrome and HIV infection: An inflammatory profile
Authors: Amador-Lara Fernando , Andrade-Villanueva Jaime F. , Vega-Magaña Natali , Peña-Rodríguez Marcela , Alvarez-Zavala Monserrat , Sanchez-Reyes Karina , Toscano-Piña Marcela , Peregrina-Lucano Alejandro A. , del Toro-Arreola Susana , González-Hernández Luz A. , Bueno-Topete Miriam R. 
Published: February 2022

Abstract

Aim
A remarkable increase in metabolic syndrome (MetS) has occurred in HIV-infected subjects. Gut dysbiosis is involved in the pathogenesis of metabolic disorders. Therefore, the aim is to explore the profile of the gut microbiota in Mexican population with HIV infection and MetS.

Methods and Results
In all, 30 HIV-infected patients with MetS were compared to a group of 30 patients without MetS, treated with integrase inhibitors and undetectable viral load were included in the study. Stool samples were analysed by 16S rRNA next-generation sequencing. High-sensitivity C-reactive protein &gt;3 mg L−1 and higher scores in cardiometabolic indices were associated with MetS. The group with MetS was characterized by a decrease in α-diversity, higher abundance of Enterobacteriaceae and Prevotella, as well as a dramatic decrease in bacteria producing short-chain fatty acids. Prevotella negatively correlated with Akkermansia, Lactobacillus and Anaerostipes. Interestingly, the group without MetS presented higher abundance of Faecalibacterium, Ruminococcus, Anaerofilum, Oscillospira and Anaerostipes. Functional pathways related to energy metabolism and inflammation were increased in the group with MetS.

Conclusions
HIV-infected patients with MetS present a strong inflammatory microbiota profile; therefore, future strategies to balance intestinal dysbiosis should be implemented.
Physiological pacing with a DF‐1 single chamber defibrillator in a patient with permanent atrial fibrillation and heart block: A case report
Authors: Nasarre Mathieu , Clementy Nicolas , Bodin Alexandre , Babuty Dominique , Bisson Arnaud 
Published: March 2022

AbstractWe report a case report of a 68‐year‐old man with chemotherapy‐induced cardiomyopathy and uncontrolled permanent atrial fibrillation. Cardiac resynchronization therapy implantation and atrioventricular junction ablation were planned. DF‐1 single chamber defibrillator was connected to lead's defibrillation and a lead destinated to left bundle branch area pacing. This system leads to reduce costs by one‐third, improve battery longevity, and provide a more physiological pacing.
Patients with hypothermic sepsis have a unique gene expression profile compared to patients with fever and sepsis
Authors: Harmon Matthew B. A. , Scicluna Brendon P. , Wiewel Maryse A. , Schultz Marcus J. , Horn Janneke , Cremer Olaf L. , van der Poll Tom , Joost Wiersinga W. , Juffermans Nicole P. ,   the MARS consortium
Published: March 2022

AbstractThe pathophysiology of hypothermia during sepsis is unclear. Using genomic profiling of blood leukocytes, we aimed to determine if hypothermia is associated with a different gene expression profile compared to fever during sepsis. Patients with sepsis and either hypothermia or fever within 24 hours after ICU admission were included in the study (n = 168). Hypothermia was defined as body temperature below 36 °C. Fever was defined as body temperature equal to or above 38.3°C. We compared blood gene expression (whole‐genome transcriptome in leukocytes) in hypothermic septic compared to febrile septic patients in an unmatched analysis and matched for APACHE IV score and the presence of shock. In total, 67 septic patients were hypothermic and 101 patients were febrile. Hypothermia was associated with a distinct gene expression profile in both unmatched and matched analyses. There were significant differences related to the up‐ and downregulation of canonical signalling pathways. In the matched analysis, the top upregulated gene was cold‐inducible mRNA binding protein (CIRBP) which plays a role in cold‐induced suppression of cell proliferation. In addition, we found three signalling pathways significantly upregulated in hypothermic patients compared to febrile patients; tryptophan degradation X, phenylalanine degradation IV and putrescine degradation III. In conclusion, there are distinct signalling pathways and genes associated with hypothermia, including tryptophan degradation and CIRBP expression, providing a possible link to the modulation of body temperature and early immunosuppression. Future studies may focus on the canonical signalling pathways presented in this paper to further investigate spontaneous hypothermia in sepsis.
New contribution to the peritrichous genus Ophrydium (Protista, Ciliophora) with notes on the morphology, taxonomy, and phylogeny of a well‐known species Ophrydium crassicaule Penard, 1922
Authors: Wu Tong , Wang Zhe , Lu Borong , Lei Jingtao , Al‐Rasheid Khaled A.S. , Sheng Yalan 
Published: March 2022

AbstractThe peritrichous genus Ophrydium is relatively distinctive since its colonies have a gelatinous lorica. In the present work, one morphologically well‐described species, Ophrydium crassicaule, was collected from a freshwater habitat in China. Both morphological characters and sequence data for SSU rDNA, ITS1‐5.8S‐ITS2 region and LSU rDNA were obtained. Morphologically, O. crassicaule is characterized by its elongated vase‐shaped zooid, single‐layered peristomial lip, dichotomously branched stalk, colony with gelatinous lorica, and infundibular polykinety 3 (P3) containing one short inner row and two long outer rows. Phylogenetic analyses revealed that the genus Ophrydium is monophyletic and nests within the family Vorticellidae. In addition, we briefly revise the nominal congeners for aiding the species identification in future studies.
The presence of ettringite in concrete affected by alkali‐silica reaction and its potential use as recycled aggregate
Authors: Piersanti Matthew , Shehata Medhat H. 
Published: February 2022

AbstractSamples were collected from a 20‐year‐old concrete suffering alkali‐silica reaction for examination using scanning electron microscopy. The concrete was also exposed to cycles of freezing and thawing in service. The old concrete was processed and used as recycled concrete aggregate in new concrete tested under lab conditions. The study shows that backscattered electron imaging, energy dispersive x‐ray analysis and x‐ray mapping are helpful tools to identify the presence of alkali‐silica reaction products. X‐ray mapping was found helpful to confirm the presence of reaction products in cases where the products or the cracking are not very clear in the collected image. The study shows the presence of ettringite in the air voids of the concrete. The formed ettringite is believed to have no direct effect on the expansion due to the alkali‐silica reaction as they form in an empty space without causing swelling pressure on the concrete. The effect of the ettringite formed in the air voids on the resistance to freezing/thawing was not confirmed in this study. Recycling the old affected concrete as aggregate in new concrete was found to cause significant expansion under lab conditions.
LncRNA KCNQ1OT1‐mediated cervical cancer progression by sponging miR‐1270 as a ceRNA of LOXL2 through PI3k/Akt pathway
Authors: Jiang Li , Jin Haihong , Gong Shan , Han Kun , Li Ze , Zhang Weihu , Tian Jing 
Published: February 2022

AbstractBackgroundDysregulated noncoding RNAs participated in progressions of cervical cancer.PurposeTo verify impacts of KCNQ1OT1 on modulating progressions of cervical cancer cells.MethodExpressions of KCNQ1OT1, miR‐1270, and LOXL2 were analyzed through RT‐qPCR and protein expressions of LOXL2, p‐AKT, and AKT were validated using western blot. Bindings of miR‐1270 with KCNQ1OT1 or LOXL2 were verified using luciferase reporter assay. CCK‐8 and flow cytometry evaluated cell viability and apoptosis, respectively. The PI3K/AKT signaling pathway suppressor, LY294002, was applied to treat the cells and the changes of KCNQ1OT1 expression and LOXL2, p‐AKT, and AKT protein expressions were examined.ResultsKCNQ1OT1 expression was the highest in HeLa cells but lowest in SiHa cells whose upregulation improved the viability but inhibited the apoptosis in SiHa cells while knockdown of KCNQ1OT1 caused opposite results in HeLa cells. MiR‐1270 was sponged and negatively modulated by KCNQ1OT1. MiR‐1270 mimics caused low viability and high apoptosis of SiHa cells but miR‐1270 inhibitor reverse its roles in HeLa cells. LOXL2, the target of miR‐1270, positively interplayed with KCNQ1OT1 but had negative interaction with miR‐1270. LOXL2 overexpression promoted viability and decreased apoptosis of SiHa cells but knockdown of LOXL2 restored its effects in HeLa cells. Moreover, LOXL2 and phosphorylated AKT (p‐AKT) protein expressions were downregulated by suppressed KCNQ1OT1 and LOXL2 and miR‐1270 mimics but promoted by overexpressed KCNQ1OT1 and LOXL2 and miR‐1270 inhibitor. Additionally, LY294002 treatment caused low KCNQ1OT1 RNA expression and decreased LOXL2 and p‐AKT protein expressions.ConclusionKCNQ1OT1/miR‐1270/LOXL2 axis modulated viability and apoptosis of cervical cancer cells.
Clinical and laboratory spectrum of inborn errors of immunity in Egypt: Five years of experience at a tertiary care university hospital
Authors: Almalky Mohamed , Abdelaziz Tarek A. , Baz Eman Gamal 
Published: February 2022

AimThe recognition and diagnosis of primary immunodeficiency disorders (PIDs) is challenging in developing countries. This study aimed to describe the features of PID patients in a tertiary care setting in Egypt and analyse the distribution, clinical features and outcome of PID among paediatric patients.MethodsThis cross‐sectional retrospective study was conducted between January 2016 and January 2021, to evaluate all paediatric patients aged below 18 years with PID that were diagnosed according to the International Union of Immunological Societies 2017 classification. We retrospectively studied the clinical features, diagnostic spectrum, laboratory investigations and relevant immunological workup, and treatment options.ResultsA total of 61 PID patients were enrolled in the current study. The median age at diagnosis was 22 months. The overall consanguinity rate was 49.2%, and the family history of PID was 19.7%. Among all PIDs, the combined immunodeficiency with syndromic features predominates with 17 cases, accounting for 27.9% of all cases of PIDs. The predominant antibody deficiency was the second common PID that was diagnosed in 14 patients (23%). Recurrent pneumonia was the most common initial presentation, occurring in 77% of patients, followed by failure to thrive (63.9%), and recurrent otitis media (55.7%). The total deaths were 18 patients (29.5%).ConclusionPaediatric patients with PIDs are not uncommon in Egypt. There is a need to improve PID diagnosis and treatment, for better estimation of PID and to decrease morbidity and mortality.
Streptococcus strain C17T as a potential probiotic candidate to modulate oral health
Authors: Zhang Wen Xiao , Xiao Chun Ling , Li Shu Yin , Bai Xiao Cui , Qi He , Tian Han , Wang Nan , Yang Biao , Li Xin Ming , Sun Ye 
Published: February 2022

Abstract
In the microbiome, probiotics modulate oral diseases. In this study, Streptococcus strain C17T was isolated from the oropharynx of a 5-year-old healthy child, and its potential probiotic properties were analysed using human bronchial epithelial cells (16-HBE) used as an in vitro oropharyngeal mucosal model. The results demonstrated that the C17T strain showed tolerance to moderate pH ranges of 4–5 and 0·5–1% bile. However, it was more tolerant to 0·5% bile than 1% bile. It also demonstrated an ability to accommodate maladaptive oropharyngeal conditions (i.e. tolerating lysozyme at 200 μg ml−1). It was also resistant to hydrogen peroxide at 0·8 mM. In addition, we found out that the strain possesses inhibitory activities against various common pathogenic bacteria. Furthermore, C17T was not cytotoxic to 16-HBE cells at different multiplicities of infection. Scanning electron microscopy disclosed that C17T adhesion to 16-HBE cells. Competition, exclusion and displacement assays showed that it had good anti-adhesive effect against S. aureus. The present study revealed that Streptococcus strain C17T is a potentially efficacious oropharyngeal probiotic.
Consensus recommendations for histological criteria of autoimmune hepatitis from the International AIH Pathology Group
Authors: Lohse Ansgar W. , Sebode Marcial , Bhathal Prithi S. , Clouston Andrew D. , Dienes Hans P. , Jain Dhanpat , Gouw Annette S. H. , Guindi Maha , Kakar Sanjay , Kleiner David E. , Krech Till , Lackner Carolin , Longerich Thomas , Saxena Romil , Terracciano Luigi , Washington Kay , Weidemann Sören , Hübscher Stefan G. , Tiniakos Dina 
Published: March 2022

AbstractBackground & AimsDiagnostic histological criteria for autoimmune hepatitis (AIH) have not been clearly established. Previously published criteria focused mainly on chronic AIH, in which inflammatory changes mainly occur in portal/periportal regions and may not be applicable to acute presentation of AIH, in which inflammatory changes are typically predominantly lobular in location. International consensus criteria for the diagnosis and assessment of disease severity in both acute and chronic AIH are thus urgently needed.MethodsSeventeen expert liver pathologists convened at an international workshop and subsequently used a modified Delphi panel approach to establish consensus criteria for the histopathological diagnosis of AIH.ResultsThe consensus view is that liver biopsy should remain standard for diagnosing AIH. AIH is considered likely, if there is a predominantly portal lymphoplasmacytic hepatitis with more than mild interface activity and/or more than mild lobular hepatitis in the absence of histological features suggestive of another liver disease. AIH is also considered likely if there is predominantly lobular hepatitis with or without centrilobular necroinflammation and at least one of the following features: portal lymphoplasmacytic hepatitis, interface hepatitis or portal‐based fibrosis, in the absence of histological features suggestive of another liver disease. Emperipolesis and hepatocellular rosettes are not regarded as being specific for AIH.ConclusionsThe criteria proposed in this consensus statement provide a uniform approach to the histological diagnosis of AIH, which is relevant for patients with an acute as well as a chronic presentation and to more accurately reflect the current understanding of liver pathology in AIH.
Influence of background characteristics in responders of regenerative therapy by periurethral injection of adipose‐derived regenerative cells for male stress urinary incontinence
Authors: Yamanishi Tomonori , Ishizuka Osamu , Shimizu Shinobu , Kobayashi Yumiko , Kinoshita Fumie , Yamamoto Tokunori , Mizokami Atushi , Narimoto Kazutaka , Toriyama Kazuhiro , Kamei Yuzuru , Kuwatsuka Yachiyo , Mizuno Masaaki , Gotoh Momokazu 
Published: February 2022

AbstractObjectivesTo determine if the male responders with post‐prostatectomy incontinence in the ADRESU study, which is a clinical trial of regenerative therapy by periurethral injection of adipose‐derived regenerative cells, are influenced by any background characteristics.MethodsBriefly, autologous adipose‐derived regenerative cells isolated from abdominal adipose tissue and a mixture of adipose‐derived regenerative cells with fat tissue were transurethrally injected into the rhabdosphincter and submucosal space of the urethra, respectively. Sixteen out of 43 patients (37.2%) responded to treatment (responders) and exhibited improvement in the urine leakage volume, defined as >50% reduction from baseline determined by the 24‐hour pad test at 52 weeks of treatment (or last visit within 52 weeks). Background data such as age, body weight, method of prostatectomy, baseline frequency of leaks, number of leaks, number of pad changes, International Consultation on Incontinence Questionnaire‐Short Form, King's Health Questionnaire, urodynamic urethral function including functional profile length and maximum urethral closure pressure, and abdominal leak point pressure were collected and compared between responders and nonresponders.ResultsNone of the background factors influenced improvement in the responders as compared with the nonresponders. However, a significant between‐group difference in the rates of decrease in urine leakage volume was noted in patients of younger age (<70 years), compared with those of older age (≥70 years) from 2 to 26 weeks of treatment.ConclusionA greater decrease in urine leakage volume was noted in the younger patient group than in the older patient group.
Monitoring nurturing care environments for early childhood from the national to the municipal level
Authors: Pérez‐Escamilla Rafael , Venancio Sonia , Buccini Gabriela 
Published: March 2022

Key messages
There is a dearth of indicators and management information systems to assess early childhood nurturing care environments.
The Brazilian Early Childhood Care Friendly Municipal Index (IMAPI) is a robust tool to assess nurturing care environments at the local level.
IMAPI was able to detect nurturing care inequities within and across regions.
Further validation work is needed to confirm the utility of IMAPI for policymaking.
Combining population genomics with demographic analyses highlights habitat patchiness and larval dispersal as determinants of connectivity in coastal fish species
Authors: Knutsen Halvor , Catarino Diana , Rogers Lauren , Sodeland Marte , Mattingsdal Morten , Jahnke Marlene , Hutchings Jeffrey A. , Mellerud Ida , Espeland Sigurd H. , Johanneson Kerstin , Roth Olivia , Hansen Michael M. , Jentoft Sissel , André Carl , Jorde Per Erik 
Published: March 2022

AbstractGene flow shapes spatial genetic structure and the potential for local adaptation. Among marine animals with nonmigratory adults, the presence or absence of a pelagic larval stage is thought to be a key determinant in shaping gene flow and the genetic structure of populations. In addition, the spatial distribution of suitable habitats is expected to influence the distribution of biological populations and their connectivity patterns. We used whole genome sequencing to study demographic history and reduced representation (double‐digest restriction associated DNA) sequencing data to analyse spatial genetic structure in broadnosed pipefish (Syngnathus typhle). Its main habitat is eelgrass beds, which are patchily distributed along the study area in southern Norway. Demographic connectivity among populations was inferred from long‐term (~30‐year) population counts that uncovered a rapid decline in spatial correlations in abundance with distance as short as ~2 km. These findings were contrasted with data for two other fish species that have a pelagic larval stage (corkwing wrasse, Symphodus melops; black goby, Gobius niger). For these latter species, we found wider spatial scales of connectivity and weaker genetic isolation‐by‐distance patterns, except where both species experienced a strong barrier to gene flow, seemingly due to lack of suitable habitat. Our findings verify expectations that a fragmented habitat and absence of a pelagic larval stage promote genetic structure, while presence of a pelagic larvae stage increases demographic connectivity and gene flow, except perhaps over extensive habitat gaps.
Interbank market and funding liquidity risk in a stock‐flow consistent model
Authors: Reale Jessica 
Published: March 2022

AbstractThe present stock‐flow consistent model aims at capturing the second causal link of endogenous monetary theory, from deposits to reserves, by including intrasectoral flows within the banking sector anddebt maturity structuredecisions. For this purpose, banks can choose the demanded duration of interbank loans, either overnight or term, according to a measure for maturity mismatch which captures funding liquidity risk. The simulations show that: (i) a well‐functioning term interbank market is needed when banks face exogenous shocks; and (ii) banks’ funding structure may act as an endogenous source of credit market pressures.
Robust comparative performance of genomic DNA extraction methods from non‐engorged phlebotomine sandflies
Authors: Senne Nathália Alves , Santos Huarrisson Azevedo , Araújo Thamires Rezende , Paulino Patrícia Gonzaga , Mendonça Leo Paulis , Moreira Haika Victória Sales , Camilo Tays Araújo , da Costa Angelo Isabele 
Published: March 2022

AbstractThe present study is a comparative analysis of DNeasy Blood & Tissue Qiagen® kit (Qiagen®, Hilden, Alemanha), salting out, HotShot and phenol–chloroform protocols to extract DNA from sandflies. In addition, a comparative test using sandflies with and without eyes evaluated the potential inhibitory effect in the cPCR. An inhibition test was performed using an exogenous DNA added to the qPCR. The genomic DNA quality of each sample was evaluated by cPCR based on the cytochrome c oxidase subunit I (cox1) gene. The DNA extraction protocols showed the following percentage of amplification: HotShot (91.6% [55/60]), salting out (71.6% [43/60]), phenol–chloroform (95% [57/60]) and kit DNeasy Blood & Tissue Qiagen® (73.3% [44/60]). The phenol–chloroform method achieved a significantly higher frequency of cox1 gene amplification. The pigment present in the phlebotomine's eyes seems to inhibit cPCR reactions since the frequency of amplification of the cox1 gene increased in the sandflies without eyes (p < 0.0001). The HotShot method showed the highest inhibitory potential. These manual extraction techniques can be an inexpensive and effective alternative to study vector–pathogen interactions.
Core arbuscular mycorrhizal fungi are predicted by their high abundance–occupancy relationship while host‐specific taxa are rare and geographically structured
Authors: Kajihara Kacie T. , Egan Cameron P. , Swift Sean O. I. , Wall Christopher B. , Muir Christopher D. , Hynson Nicole A. 
Published: February 2022

Summary

Habitat restoration may depend on the recovery of plant microbial symbionts such as arbuscular mycorrhizal (AM) fungi, but this requires a better understanding of the rules that govern their community assembly.

We examined the interactions of soil and host‐associated AM fungal communities between remnant and restored patches of subtropical montane forests.

While AM fungal richness did not differ between habitat types, community membership did and was influenced by geography, habitat and host. These differences were largely driven by rare host‐specific AM fungi that displayed near‐complete turnover between forest types, while core AM fungal taxa were highly abundant and ubiquitous. The bipartite networks in the remnant forest were more specialized and hosts more specific than in the restored forest. Host‐associated AM fungal communities nested within soil communities in both habitats, but only significantly so in the restored forest.

Our results provide evidence that restored and remnant forests harbour the same core fungal symbionts, while rare host‐specific taxa differ, and that geography, host identity and taxonomic resolution strongly affect the observed distribution patterns of these fungi. We suggest that host‐specific interactions with AM fungi, as well as spatial processes, should be explicitly considered to effectively re‐establish target host and symbiont communities.