bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of artificial neural network for prediction of fluoride removal efficiency using neutralized activated red mud from aqueous medium in a continuous fixed bed column.
Authors: Giri Anil Kumar , Mishra Prakash Chandra 
Published: June 2022

Abstract
The present research work approaches the removal of fluoride from aqueous medium using neutralized activated red mud (NARM) in a continuous fixed bed column. Artificial neural network (ANN) technique was applied effectively for optimization of the model for the practicability of the removal process. The consequences of various experimental variables like bed length, adsorbate concentration, experimental time and adsorbate solution flow rate studied to know the breakthrough point and saturation times. The highest removal potentiality of NARM was considered to be 3.815 mg g− 1 of F− in the bed height of 15 cm, starting concentration 1ppm, susceptible time 120 minutes, adsorbate solution flow rate 0.5 mLmin− 1, and constant room temperature, respectively. Bohart-Adams and Thomas models were considered to describe the fixed bed column effect to the bed height and adsorbate concentrations. The experimental data were applied a back propagation (BP) learning algorithm programme with four-seven-one architecture model. The artificial neural network model was considered to be functioning correctly as absolute relative percentage error, which is 0.671 throughout the learning period. Differentiation between the predicted out comes from ANN model and actual results from experimental analysis affords a high degree of correlation (R2 = 0.998) stipulating that the model was able to predict the adsorption efficiency. Experimented adsorbent materials was characterized using different instrumental analysis that is SEM-EDS, FTIR and XRD.
The sleep EEG envelope: a novel, neuronal firing-based human biomarker
Authors: Ujma Péter P. , Dresler Martin , Simor Péter , Fabó Dániel , Ulbert István , Erőss Loránd , Bódizs Róbert 
Published: June 2022

Abstract
Sleep EEG reflects voltage differences relative to a reference, while its spectrum reflects its composition of various frequencies. In contrast, the envelope of the sleep EEG reflects the instantaneous amplitude of oscillations, while its spectrum reflects the rhythmicity of the occurrence of these oscillations. The sleep EEG spectrum is known to relate to demographic, psychological and clinical characteristics, but the envelope spectrum has been rarely studied. In study 1, we demonstrate in human invasive data from cortex-penetrating microelectrodes and subdural grids that the sleep EEG envelope spectrum reflects neuronal firing. In study 2, we demonstrate that the scalp EEG envelope spectrum is stable within individuals. A multivariate learning algorithm could predict age (r = 0.6) and sex (r = 0.5) from the EEG envelope spectrum. With age, oscillations shifted from a 4–5 second rhythm to faster rhythms. Our results demonstrate that the sleep envelope spectrum is a promising biomarker of demographic and disease-related phenotypes.
A nexus study of carbon emissions and financial development in China using the decoupling analysis
Authors: Yuan Rong , Liao Haoyun , Wang Juan 
Published: June 2022

Abstract
Investigating the linkage between financial development (FD) and carbon emissions is important for mitigating climate change, nevertheless, how carbon emissions decouple from FD is still unclear. Here, we investigate the relationship between FD and carbon emissions by using the decoupling model based on cross-province data of China during 2000–2019. Then we use the decomposition method to analyze the nine drivers of decoupling elasticity of FD and CO2 emissions. We found that China experienced weak decoupling and strong negative decoupling in most years. Only the finance develops at a very high level, the FD had spare capacities to promote the reduction in the carbon emissions. For example, several developed provinces (e.g., Tianjin, Zhejiang, Guangdong) realized strong decoupling after 2012. The reduction in energy intensity and the increase of foreign direct investment promoted the decoupling of FD from carbon emissions. During the financial recession period, developing a bank-based financial market helped the emissions reduction. Once financial crisis is overcome, developing a market-based financial market promoted the decoupling of FD from emissions. This is because that with the fast FD, the development of stock market contributed to emission reductions through technological improvement, while the bank loans inhibited the decoupling process through the expansion of capital-labor inputs.
A Security Method of Hardware Trojan Detection Using Path Tracking Algorithm
Authors: Huang Der-Chen , Hsiao Chun-Fang , Chang Tin-Wei , Chu Ying-Yi 
Published: June 2022

Abstract
Recently, the issue of malicious circuit alteration and attack draws more attention than ever before due to the globalization of IC design and manufacturing. Malicious circuits, also known as hardware Trojans, are found able to degrade the circuit performance or even leak confidential information, and accordingly it is definitely an issue of immediate concern to develop detection techniques against hardware Trojans. This paper presents a ring oscillator-based detection technique to improve the hardware Trojan detection performance. A circuit under test is divided into a great number of blocks, path assignment is optimized using a path tracking algorithm, and a high coverage is reached accordingly.
Cervical cancer, the human papillomavirus vaccine, and cervical screenings from 2004—2021: a Google Trends analysis in Japan
Authors: Niu Qian , Liu Junyu , Kawaguchi Asuka , Yoshinari Yuka , Nakata Sakura , Aoyama Tomoki , Nagai-Tanima Momoko 
Published: June 2022

Abstract
Background
The World Health Organization (WHO) established a global strategy to accelerate the elimination of cervical cancer in 2020; fortunately, it can be effectively controlled or prevented by the human papillomavirus (HPV) vaccine. Nevertheless, Japan has been experiencing an HPV vaccine crisis since 2013, with a vaccination rate of less than 1% lasting for seven years. We examined trend changes related to cervical cancer topics and assessed the feasibility and limitations of Google Trends as an indicator of public health surveillance.
Methods
We collected search query data from Google Trends and the annual number of cervical cancer cases from the National Cancer Center, Japan. We then conducted a chronological and geographical analysis of the public interest in the phrases “cervical cancer,” “HPV vaccine,” and “cervical screening” from 2004—2021. We calculated correlations between the annual number of cervical cancer cases and the search terms. We also detected change points in public search interest. Finally, we analyzed subregional-level search interest disparities.
Results
Japanese public search interest in “cervical cancer,” “HPV vaccine,” and “cervical screening” increased from 2010—2013. Subsequently, interest in “cervical cancer” and “cervical screening” remained stable and relatively high, while interest in “HPV vaccine” was suddenly minimal. The actual number of cervical cancer cases showed a strong correlation with search volumes for “cervical screening” (r = 0.953, p < .001) and “cervical cancer” (r = 0.898, p < .001), respectively. In 2020, the public reignited its interest in “HPV vaccine.” We detected six change points in these three topics around 2010, 2013, and 2020, which aligned with critical policy decisions. Prefectures’ public interest levels were different for these three topics.
Conclusions
The Japanese public retained low interest in “HPV vaccine” from 2013—2020, while interest in “cervical cancer” and “cervical screening” terms was relatively high. Encouragingly, the public reignited its interest in “HPV vaccine” in 2020, which is expected to promote vaccination. Google Trends can extend unique perspectives for authorities to understand public concerns nearly synchronously, but it has a limited interpretation in subregions.
Expansion of the osteocytic lacunar-canalicular system involved in pharmacological action of PTH revealed by AI-driven fluorescence morphometry in rabbits
Authors: Takakura Aya , Sato Takanori , Lee Ji-Won , Hirano Kyoko , Takao-Kawabata Ryoko , Ishizuya Toshinori , Iimura Tadahiro 
Published: June 2022

Abstract
Osteoporosis is an age-related disorder that is characterized by reduced bone mass. Its prevention and treatment are important healthcare issues for maintaining social activity in aged societies. Although bone fractures mostly occur at sites of weakened cortical bone, pathophysiological and pharmacological evaluations of bone mass have tended to be predominantly assessed in trabecular bone. To statistically characterize cortical bone remodeling, we originally established multimode fluorescence imaging and artificial intelligence-driven morphometric analyses in rabbits with well-defined cortical remodeling, similar to that in humans. We evaluated three distinct administration frequencies of teriparatide [TPTD; human parathyroid hormone, hPTH (1–34)]: once (1/w), twice (2/w), and seven times (7/w) a week, with the same total dose (140 μg/kg/week). Our analyses revealed significant expansions of the osteocytic lacunar-canalicular system and Haversian canals accompanied by the development of cortical porosity and endosteal naïve bone formation induced by a frequent administration regimen (7/w) of TPTD; however, once-weekly (1/w) and twice-weekly (2/w) administration of TPTD showed little effect. These findings demonstrate a clear contrast between the effects of frequent and infrequent administration of TPTD on cortical bone metabolism and suggest that osteocytic bone remodeling is involved in the pharmacological action of PTH.
VPC: A time-efficient control method for a hexapod wheel-legged robot based on velocity planning
Authors: Sun Junkai , Sun Zezhou , Li Jianfei , Wang Chu , Jing Xin , Wei Qingqing , Liu Bin , Yan Chuliang 
Published: June 2022

Abstract
Aiming at the problem that control methods of wheel-legged robot for future Mars exploration missions are too complex, a time-efficient control method based on velocity planning for a hexapod wheel-legged robot is proposed in this paper, which is named as velocity planning control. When foot end or wheel at knee contacts ground, the desired velocity of foot end or knee is transformed according to the velocity transformation of rigid body from the desired velocity of torso which is obtained by the deviation of torso position and posture. Furthermore, the torques of joints can be obtained by impedance control. When suspended, the leg is regarded as a system consisted of a virtual spring and a virtual damper to realize control of legs in the swing phase. Besides, leg sequences of switching motion between wheeled configuration and legged configuration are planned. According to complexity analysis, velocity planning control has lower time complexity and less times of multiplication and addition compared with virtual model control. Besides, simulations show that velocity planning control can realize stable periodic gait motion, wheel-leg switching motion and wheeled motion and the operation time of velocity planning control is about 33.89% less than that of virtual model control, which promises a great prospect for velocity planning control in future planetary exploration mission.
Targeted Modulation of Inflammatory Lymphocytes and Neurogenesis/Neuroprotection Associated Genes by Curcumin and a Novel Derivative of Curcumin on Mice Experimental Autoimmune Encephalomyelitis Model
Authors: Khosropour Saeid , Noori Shokoofe , Salehi Niloufar , Rezaeizadeh Hossein , Esmaeelzadeh Maryam , Salehi Peyman , Nikzamir Abdolrahim , Zarghi Afshin 
Published: June 2022

Abstract
Multiple sclerosis (MS) is a chronic demyelinating disease that causes neurological disabilities mostly in young adults. Curcumin (CUR), a polyphenol found in Turmeric, has anti-inflammatory and neuroprotective properties in autoimmune diseases including MS, although it has low bioavailability and solubility. In the present study, administration of CUR and FCUR, a potent synthetic derivative of CUR, on experimental autoimmune encephalomyelitis (EAE), animal model of MS, was investigated. MOG-induced EAE mice were treated with placebo, 4 mg/Kg CUR, or 4 mg/Kg FCUR 10 days post immunization. After 20 days of treatment, mice were euthanized and subjected to ex-vivo examination. Protein was detected with ELISA and western blot. mRNA level was measured with qRT-PCR. The results showed that compared with the control group, in both CUR and FCUR treated groups, clinical score was improved. Histological H&E staining of spinal cord tissue demonstrated reduction in lymphocyte infiltration. Cytokine production of IL-6 and IL-17 decreased and TGF-β increased in the splenocytes and serum. Also the compounds stimulated mRNA expression of IL-4, IL-10, TGF-β, FOXP3, GATA3, MBP, and Olig2, and inhibited IL-1, IL-17, IFN-γ, RORγt, and T-bet genes in spinal cord lymphocytes. However, no considerable effect on PDGFRα, nestin, GAS6, Tyro3, and MERTK regulation was observed. The reported results were more significant in FCUR treated group. In conclusion, this study suggests that CUR and FCUR can alleviate neurological symptoms, reduce inflammation, and increase remyelination and neuroprotection. However, the stronger preliminary efficacy of FCUR would render this newly synthesized derivative as a potent therapeutic candidate against MS.
The Ferroptosis Inhibitor Liproxstatin-1 Ameliorates LPS-Induced Cognitive Impairment in Mice
Authors: Li Yang , Sun Miao , Zhang Linlin , Ma Yulong , Li Hao , Cao Jiangbei , Song Jie , Mi Weidong , Zhang Xiaoying 
Published: June 2022

Abstract
The CNS inflammation is known to play a significant role in the mechanism of perioperative neurocognitive disorder (PND) and iron homeostasis imbalance was reported to involve in this process by participating oxidative stress. Ferroptosis is a newly discovered, iron-dependent form of cell death which occurs in multiple neurodegenerative diseases characterized by cognitive dysfunction. However, the effect of ferroptosis in inflammation-related PND is unknown. In this study, we explored the role of ferroptosis in a mouse model of LPS-induced cognitive impairment. The results showed that ferroptosis inhibitor liproxstatin-1 ameliorated LPS-induced memory impairment in MWM by attenuating ROS reaction (SOD, MDA) and lipid peroxidation (LPO), decreasing the activation of microglia and the inflammatory cytokines (IL-6, TNF-α). The iron deposition (iron content) and the expression alteration of ferroptosis-related proteins (TF, xCT, Fth, Gpx4, FtMt) were alleviated, and the mitochondrial injury and neuronal damage were weakened by liproxstatin-1 compared to the LPS model group. These findings suggested that ferroptosis could participate in the mechanism underlying LPS-induced cognitive dysfunction by taking part in iron metabolism imbalance, motivating oxidative stress, activating inflammatory cascade response and contributing further to neuronal damage.
Estimating Critical Value from Electrocardiogram using a Deep Ordinal Convolutional Neural Network
Authors: Wei Guodong , Di Xinxin , Zhang Wenrui , Geng Shijia , Zhang Deyun , Wang Kai , Fu Zhaoji , Hong Shenda 
Published: June 2022

Abstract
Critical values are commonly used in clinical laboratory tests to define health-related conditions of varying degrees. Knowing the values, people can quickly become aware of health risks, and the health professionals can take immediate actions and save lives. In this paper, we propose a method that extends the concept of critical value to one of the most commonly used physiological signals in the clinical environment - Electrocardiogram (ECG). We first construct a mapping from common ECG diagnostic conclusions to critical values. After that, we build a 61-layer deep convolutional neural network named CardioV, which is characterized by an ordinal classifier. We conduct experiments on a recently released large ECG dataset, and demonstrate that CardioV achieves a mean absolute error of 0.4984 and a ROC-AUC score of 0.8735. In addition, we find that the model performs better for extreme critical values and the younger age group, while gender does not affect the performance. The ablation study confirms that the ordinal classification mechanism suits for estimating the critical values which contain ranking information. Moreover, model interpretation techniques help us discover that CardioV focuses on the characteristic ECG locations during the critical value estimation process.
SIRT1's O-GlcNAcylation protects cold exposed-induced skeletal muscle damage through ameliorates mitochondrial homeostasis
Authors: Cao Yu , Zhang Meng , Li Ye , Lu Jing-Jing , Zhou Wan-Hui , Li Xiao-Shuag , Shi Hao , Xu Bin , Li Shi-Ze 
Published: June 2022

Abstract
Objective: O-GlcNAcylation, a nutrient sensing pathway involved in a myriad of cellular processes, it plays a key role in metabolism homeostasis. Cold stress disturbs cellular metabolic and energy homeostasis and is one of the causes of stress-induced illnesses. It is reported that O-GlcNAcylation has been shown to be upregulated during cold stress. Skeletal muscle and its residential mitochondria play an important role in maintaining the metabolic homeostasis of the whole body. Nevertheless, the mechanism of which O-GlcNAcylation adapts to cold stress in skeletal muscle remains unknown.Methods: To characterize the effect of O-GlcNAcylation on mouse skeletal muscle during cold exposure, this research investigated the effects of skeletal muscle structure, function, mitochondrial homeostasis, the deacetylation activity of SIRT1, acetylation expression levels and oxidative stress levels in Ogt mKO mice by cold exposure (4 ℃, 3 h a day, for 1 week). In order to understand the mechanism of O-GlcNAcylation on skeletal muscle homeostasis under cold stress, C2C12 cells was used as a model in vitro. C2C12 cells were treated with OGT inhibitor (Alloxan) and OGA inhibitor (Thiamet G) to decrease and enhance O-GlcNAcylation signals in mild hypothermia, respectively, and then recapitulated in vivo phenotype. Finally, the interaction between OGT and SIRT1 was demonstrated, and the O-GlcNAcylation of SIRT1 played an important role in the imbalance of skeletal muscle homeostasis induced by cold exposure.Results: Results showed that in addition to enhance cold stress-induced mitochondrial abnormalities, cell senescence, and collagen accumulation, Ogt deficiency was found to induce autophagy, mitophagy and oxidative stress level in skeletal muscle during cold exposure. And the results showed that the expression level of SIRT1 was reduced, concomitant with increased expression and acetylation of FoxO1 in wild type mice challenged with cold stress. Because the depletion of Ogt further reduced SIRT1, and increased FoxO1 in muscle challenged with cold stress. Furthermore, this study showed that OGT had a physiological interaction with SIRT1. Thr160 and Ser161 sites of SIRT1 could be O-GlcNAcylation, and overexpression of SIRT1 saved mitochondrial defects in C2C12 cells caused by cold exposure.Conclusion: Cold stress can cause skeletal muscle damage, and O-GlcNAcylation of SIRT1 is an important protection mechanisms of skeletal muscle’s adaptation to cold stress.
Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris
Authors: Zhang Kun , Duan Xingpeng , Cai Peng , Gao Linhui , Wu Xiaoyan , Yao Lun , Zhou Yongjin 
Published: June 2022

Abstract
Background
The methylotrophic yeast Pichia pastoris is considered as an ideal host for the production of recombinant proteins and chemicals. However, low homologous recombination (HR) efficiency hinders its precise and extensive genetic manipulation. To enhance the homology-directed repair over non-homologous end joining (NHEJ), we expressed five exonucleases that were fused with the Cas9 for enhancing end resection of double strand breaks (DSBs) of DNA cuts.
Results
The endogenous exonuclease Mre11 and Exo1 showed the highest positive rates in seamless deletion of FAA1, and fusing the MRE11 to the C-terminal of CAS9 had the highest positive rate and relatively high number of clones. We observed that expression of CAS9-MRE11 significantly improved positive rates when simultaneously seamless deletion of double genes (from 76.7–86.7%) and three genes (from 10.8–16.7%) when overexpressing RAD52. Furthermore, MRE11 overexpression significantly improved the genomic integration of multi-fragments with higher positive rate and clone number.
Conclusions
Fusion expression of the endogenous exonuclease Mre11 with Cas9 enhances homologous recombination efficiency in P. pastoris. The strategy described here should facilitate the metabolic engineering of P. pastoris toward high-level production of value-added compounds.
Nuclear localization of platelet-activating factor receptor accounts for microglial phagocytosis in ischemic stroke
Authors: Yang Jin , Ji Juan , Sun Yu-Qin , Li Shang-Ze , Jiang Zi-Kai , Dong Yin-Feng , Sun Xiu-Lan 
Published: June 2022

Abstract
Platelet-activating factor receptor (PAFR), known as a G-protein-coupled receptor (GPCR), is extensively expressed in the plasma membrane of several cells. Several studies showed that nuclear PAFR performs distinct functions from cell surface. Our previous studies showed that inhibition of microglial PAFR by miR-98 overexpression can protect salvageable neurons from microglial phagocytosis in ischemic stroke; however, the underlying mechanism remains to be fully elucidated. Herein, we created PAFR knockout mice by Crispr/Cas9-mediated genome engineering and performed in vitro studies to determine whether nuclear PAFR is a crucial regulator of microglial phagocytosis in ischemic stroke. Our results demonstrated that nuclear PAFR recruited transcription factor Specificity protein 1 (Sp1) to trigger gene transcription of milk fat globule EGF factor 8 (MFG-E8), which binds to the exposed phosphatidylserine, mediates primary phagocytosis of viable neurons during inflammation, and, in turn, enhances microglial phagocytosis. Membrane-permeable PAFR antagonist Apafant had greater therapeutic effects than membrane-impermeable antagonist Ginkgolide B in ischemic stroke. Our findings provide an explanation for the nuclear PAFR-mediated microglial phagocytosis and have implications for the selection of PAFR antagonists for use in the treatment of ischemic stroke based on the nuclear receptors target.