bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and genotype distribution of human papillomavirus in outpatients and healthy female subjects in Wenzhou, Zhejiang Province, China
Authors: Chen Qiong , Qu Wanglei , Zhao Yu , Shu Li , Wang Yi , Chen Xiangnan 
Published: November 2022

Abstract
The present study examined the prevalence of human papillomavirus (HPV) and its genotype distribution in outpatients and healthy female subjects in Wenzhou so as to develop local HPV vaccination strategies and contribute to the prevention of this disease. The present retrospective study enrolled 164,137 women, including 118,484 outpatients and 45,653 healthy female subjects from 2015 to 2020. Cervical exfoliated cells were collected from these women for DNA extraction. The DNA samples were detected with a fluorescence in situ hybridization method and 27 HPV genotypes were analyzed. The overall prevalence of HPV was 17.35%; this corresponded to a prevalence of 19.10% in the outpatient group and 12.82% in the healthy female group. HPVs 52, 58, 16, 53, and 61 were the five most prevalent HPV genotypes in the outpatient group. The five most common genotypes were HPV 52, 53, 58, 61, and 81 in the healthy female group. The HPV infection peak was estimated to be 44.65% in the 10–19 age group and 27.35% in the 60–69 age group. The burden of HPV infection in this area was high; therefore, a scientific and reasonable vaccination strategy should be adopted. The universal use of multivalent vaccines is recommended and considerable attention should be paid to HPV types that are not targeted by the vaccines. Cervical screening should be performed routinely in patients with gynecological clinical symptoms to avoid cervical intraepithelial neoplasia following persistent infection of high-risk HPV, notably in women over 60 years of age.
Effects of land use on habitat quality based on INVEST model:A Case Study of the Zuli River Basin,china
Authors: Zhang Xiaodong , Liu Xuelu 
Published: November 2022

Abstract
With the development of urbanization,the intensity of human use of land is increasing,which in turn has an impact on habitat quality. Therefore, the study of synergistic changes in land use and habitat quality plays an important role in watershed ecological protection and high-quality development.Based on the data of remote sensing in the five phases of 1980,1990,2000,2010 and 2020,the paper studies land use changes, habitat quality changes and synergistic changes in the Zuli River Basin.(1)The land use type of Zuli River basin is dominated by grassland and arable land, which account for more than 90% of the total area of the basin;(2)The rate of change in watershed land use from 1980 to 2020 is Water > Construction Land > Woodland > Grassland > Arable Land, The rate of increase of water, construction land and forest land was 20.38%,6.97% and 5.23%, respectively.The reduction rates of cultivated land and grassland were 0.27% and 0.2% respectively;(3)land use transfer in the Zuli River Basin was divided into two phases. From 1980 to 2000, with the transfer of arable land and grassland, and the transfer of woodland and construction land, cultivated land and grassland diversions were 12.90km2 and 38.19km2.The transfer volume of woodland and construction land is 16.62 km2 and 24 km2 2000 to 2020 respectively. Grassland, woodland, construction land and water diversion, 96.78 km2;(4)the overall habitat quality of the Zuli River basin increased in 1980–2020.Average habitat quality scores were 0.502, 0.5, 0.49, 0.504 and 0.52 respectively,but from the point of view of the development process were reduced and then increased;(5)There is a synergistic relationship between land use and habitat quality, and the change of grassland area in the basin influences the change of habitat quality,which is expected to provide a basis for ecological protection, land use planning and ecological protection and high-quality development of the Yellow River basin. This study aims to provide a basis for ecological protection, land use planning and ecological protection and quality development of the Yellow River basin.
WITHDRAWN: RNF125 attenuates hepatocellular carcinoma progression by downregulating SRSF1-ERK pathway
Authors: Ma Yong , Feng Zhigang , Ke Shanjia , Wang Chaoqun , Lu Shounan , Xu Yanan , Yu Hongjun , Li Zihao , Yin Bing , Li Xinglong , Hua Yongliang , Qian Baolin , Bai Miaoyu , Fu Yao , Zhang Yingmei , Wu Yaohua 
Published: November 2022

Abstract
Hepatocellular carcinoma (HCC) is one of the most deadly malignant cancers worldwide. Research into the crucial genes responsible for maintaining the aggressive behaviour of cancer cells is important for the clinical treatment of HCC. The purpose of this study was to determine whether the E3 ubiquitin ligase Ring Finger Protein 125 (RNF125) plays a role in the proliferation and metastasis of HCC. RNF125 expression in human HCC samples and cell lines was investigated using TCGA dataset mining, qRT‒PCR, western blot, and immunohistochemistry assays. In addition, 80 patients with HCC were studied for the clinical value of RNF125. Furthermore, the molecular mechanism by which RNF125 contributes to hepatocellular carcinoma progression was determined with mass spectrometry, coimmunoprecipitation (Co-IP), dual-luciferase reporter assays, and ubiquitin ladder assays. We found that RNF125 was markedly downregulated in HCC tumour tissues, which was associated with a poor prognosis for patients with HCC. Moreover, the overexpression of RNF125 inhibited HCC proliferation and metastasis both in vitro and in vivo, whereas the knockdown of RNF125 exerted antithetical effects. Mechanistically, mass spectrometry analysis revealed a protein interaction between RNF125 and SRSF1, and RNF125 accelerated the proteasome-mediated degradation of SRSF1, which impeded HCC progression by inhibiting the ERK signalling pathway. Furthermore, RNF125 was detected to be the downstream target of miR-103a-3p. In this study, we identified that RNF125 is a tumour suppressor in HCC and inhibits HCC progression by inhibiting the SRSF1/ERK pathway. These findings provide a promising treatment target for HCC.
A Comprehensive Comparison of Swarm Optimization-based Extreme Learning Machines to Predict Compression Index of Clay
Authors: Thieu Nguyen Van , Zhou Jian , Romulus Costache , Tran Trung Tin 
Published: November 2022

Abstract
Soft ground improvement is a considerable concern of many researchers worldwide in geotechnical works. In this study, the compressibility of clay (Cc) was considered for compacting the soil for soft ground improvement, and various novel intelligence models have predicted it. Indeed, a dataset containing 739 samples in the laboratory was investigated and used to develop intelligence models for predicting Cc. The extreme learning machine (ELM) was selected for this task. It was then optimized by six metaheuristic algorithms, including particle swarm optimization (PSO), moth search optimization (MSO), firefly optimization (FO), cuckoo search optimization (CSO), bees optimization (BO), and ant colony optimization (ACO), named as PSO-ELM, MSO-ELM, FO-ELM, CSO-ELM, BO-ELM, and ACO-ELM models. We used 517 samples (~ 70%) to develop models and 222 samples (~ 30%) to test the accuracy of those models. The results indicated that the accuracies of hybrid meta-heuristic-based ELM models improved from 3–5% compared to the original ELM model in predicting Cc. The highest accuracy of 87% was also reported in this study with the BO-ELM model when predicting Cc on the testing dataset. It was introduced as a robust model for predicting Cc in practical engineering that can assist in improving the soft ground.
Akkermansia muciniphila alleviates colonic epithelial endoplasmic reticulum stress through activation of farnesoid X receptors in murine experimental colitis
Authors: Bu Fan , Zhou Qiao , Dong Yuwei , Bao Han , Lu Zhihua , Yuan Xiaomin , Chen Chen , Jiang Feng , Tao Yu , Zhang Wei , Zhang Dan , Chen Yugen , Wang Qiong 
Published: November 2022

Abstract
Background
Endoplasmic reticulum (ER) stress-related mucin depletion could be involved in the pathogenesis of inflammatory bowel disease (IBD). Akkermansia muciniphila (A. muciniphila), a symbiotic bacterium of the mucus layer, uses mucin as its sole energy source and shows potential in the treatment of colitis. However, the effects and underlying mechanisms of A. muciniphila on colonic epithelial ER stress in colitis are largely unknown.
Methods
Colitis was induced by adding 2.5% DSS in drinking water. Mice were orally administered A. muciniphila (3*10^7, 3*10^8 cfu/day) once daily for 10 days during DSS intervention. UHPLC high-resolution orbitrap mass spectrometry-based metabolomic analyses were performed on faeces. 16S rRNA sequencing were used to quantify and characterize the gut microbiota of mice and human. Colons were collected from mice and analyzed by histopathology, quantitative PCR and immunofluorescence. Colon biopsies from the patients with ulcerative colitis (UC) and controls were collected and analyzed by immunohistochemistry.
Results
Metabolite pathway enrichment analysis demonstrated that colitis-affected metabolites after A. muciniphila supplementation were mainly enriched in mineral absorption, bile secretion and protein digestion and absorption. P-hydroxyphenyl acetic acid, which showed the highest VIP scores, was significantly increased by A. muciniphila, and could cause ER stress. A. muciniphila supplementation changed the relative abundance and composition of intestinal microbiotaespecially a decrease inParasutterella, which showed the potential role in bile acid maintenance. A. muciniphila supplementation protected colon shortening, histological injury, intestinal inflammation and barrier damage in wild-type (WT) mice but not in farnesoid X receptor-null (FXR−/−) mice. Mechanistically, A. muciniphila supplementation activated FXR/SHP signaling, which directly increased X-box binding protein-1 splicing (XBP1s) and phosphorylated inositol requiring enzyme 1α (p-IRE1α) expression, and in turn formed XBP1s-SHP regulatory loop in response to ER stress. We further showed that the abundance of A. muciniphila in faeces from UC patients positively correlates with p-IRE1α expression.
Conclusions
Our results suggest that A. muciniphila supplementation alleviates DSS-induced colitis involvement of the IRE1α/XBP1 ER stress pathway via FXR/SHP axis activation.
Biochemical response of human endothelial and fibroblast cells to silver nanoparticles
Authors: Palomares Cristofher Victor Vivas , Santos Jennifer Adriane , Barreto Yan Borges , Toma Sergio Hiroshi , Santos Jonnatan Julival , Stephano Marco Antonio , Oliveira Cristiano Luis Pinto , Araki Koiti , Alencar Adriano Mesquita , Bloise Antonio Carlos 
Published: November 2022

Abstract
Nowadays the bactericidal and antimicrobial properties of silver nanoparticles, AgNPs, in addition to their cytotoxic effects, have been explored to properly modulate cell biochemistry processes in order to improve the healing of wounds. Herein we investigate the cytotoxicity and metabolic profiling of two human cell lineages, the fibroblast FN1 and endothelial HUV-EC-C, planning doses of AgNPs and incubation times. Cytotoxicity assays showed consistent decrease in proliferation rates, viable cells number, and average surface areas. Metabolomics based on proton Nuclear Magnetic Resonance was successfully used to obtain quantitative and qualitative changes in metabolic events triggered by silver treatments. The metabolic profiling provided by endo- and exometabolome revealed biochemical changes induced on treated cells compared to controls. Glycolytic pathway is up-regulated due to the elevation in glucose consumption; however, the consequent elevation in pyruvate production seems to be wasted by cells to generate energy by aerobic means that are choosing to oxidize it to acetate. Aminoacid metabolism is down-regulated, signalizing the protein degradation mechanism. Tricarboxylic Acid Cycle is also down-regulated, indicating a starvation situation once succinate was left over in the culture media. Concurrently, the ketogenic pathway is up-regulated due to the excess of acetone. Changes in pyroglutamate metabolism were detected indicating the up-regulation of glutathione biosynthesis used to equilibrate the effects induced by oxidative stress, in accordance with the N-Acetylcysteine finds. Phospholipid metabolism is down-regulated, as revealed by the changes in O-Phosphocholine and Sn-Glycerol-3-PC levels, signaling reduction in the cellular proliferation rates. To the best of our knowledge, this is the first report describing AgNP-induced changes in the UDP-GlcNAc levels, which plays an essential role in modifying nucleocytoplasmic proteins. In summary, AgNPs can induce oxidative stress and cytotoxicity in endothelial and fibroblast cells, influencing their endo- and exometabolome.
Diabetes and periodontitis: Bi-directional association in population-based 15-year retrospective cohorts
Authors: Chien Wu-Chien , Fu Earl , Chung Chi-Hsiang , Cheng Chia-Mao , Tu Hsiao-Pei , Lee Wei-Cheng , Chen Wei-Liang , Shih Kuang-Chung 
Published: November 2022

Abstract
Two-way relationship between periodontitis and diabetes was advocated; however, bidirectional observation in general population is still inconclusive. Using the Taiwan Health Insurance Database (covering over 99% of the entire population),11,011 patients with severe periodontitis were recruited from 2000 to 2015.After matching by age, sex, and index date, 11,011 patients with mild periodontitis and 11,011 non-periodontitis controls were registered. The outcome of T2DM was traced. Conversely, the development of periodontitis was traced in 157,798 patients with T2DM, and 157,798 non-diabetic controls enrolled. The risks of T2DM significantly increased in groups with severe and mild periodontitis, with the adjusted hazard ratio (aHR) and 95% confidence interval (CI) being 1.94 (1.49–2.63, p < 0.01) and 1.72 (1.24–2.52, p < 0.01), respectively. Patients with severe periodontitis had a high risk of having diabetes compared to those with mild periodontitis [aHR, 1.17 (95% CI 1.04–1.26, p < 0.001)]. Conversely, the risk of periodontitis increased significantly in patients with T2DM [1.99 (1.42–2.48, p < 0.01)]. However, the high risk was not observed for the outcome of mild periodontitis [0.97 (0.38–1.57, p = 0.462)]. We, therefore, suggested the bi-direction is between diabetes and severe periodontitis, but not in mild type.
CDK4/6i treatment induces ferroptosis via downregulation of SLC7A11 mediated by SP1 for estrogen receptor positive breast cancers.
Authors: Xiaosong Li , Yingshu Cui , Xu Yuanyuang , Yuanyuan Sun , Liu Xinxin , Junwen Ju , Shan Long , Xiaofeng Kang , Zhijia Sun , Yimeng Du , Xu Xiaojie 
Published: November 2022

Abstract
Purpose: Cyclin-dependent kinase 4 and 6 inhibitors (CDK4/6is) can significantly extend tumor response in patients with metastatic estrogen receptor–positive (ER+) breast cancer, but intrinsic and acquired resistance is common. Elucidation of the molecular features of CDK4/6i sensitivity and the efficiency of their combination with novel targeted cell death inducers may pave the way toward improving patient outcomes.
Experimental Design: Ferroptosis-related characteristics were observed following treatment with the CDK4/6 inhibitor palbociclib. Transcriptomic analyses and functional assays were performed to determine the targets and regulatory mechanism of palbociclib in the ferroptosis pathway in ER+ breast cancer cell lines. A tumor xenograft model was used to study the synergistic antitumor effects of CDK4/6is in combination with ferroptosis inducers.
Results: Ferroptosis, a form of regulated cell death driven by iron-dependent phospholipid peroxidation, is partly responsible for the efficacy of the CDK4/6 inhibitor palbociclib. Mechanistically, palbociclib downregulates cystine transporter SLC7A11 by inhibiting SP1 binding to the promoter region of SLC7A11. Furthermore, genetic or pharmacological inhibitors of SP1 or SLC7A11 can enhance cell sensitivity to palbociclib and synergistically inhibit ER+ breast cancer cell growth in combination with palbociclib. A syngeneic ER+ mouse mammary tumor model was used to verify that combined inhibition of SLC7A11 or SP1 and CDK4/6 resulted in marked suppression of tumor growth in vivo.
Conclusions: Experimentally, ferroptosis represents some of the CDK4/6i-induced cell death response. This study illustrates the potential for targeting SLC7A11 in combination with CDK4/6 inhibitors and supports the investigation of combination therapy in ER+ breast cancer.
3,4-benzo[a]pyrene aggravates myocardial infarction injury by activating NLRP3-related pyroptosis through PINK1/Parkin-mitophagy-mPTP opening axis
Authors: Wu Bo-sen , Xiang Hua-qiang , Yu Yong-wei , Liu Shuai , Song Dong-yan , Lin Zhi-hui , Zhu Chen-xi , Wu Chang , Xue Yang-jing , Ji Kang-ting 
Published: November 2022

Abstract
Acute myocardial infarction(AMI) accounts for more than one third of ischemic heart disease deaths. Air pollution exposure,even for a short-term exposure,is conspicuously relevant to increased risk of MI mortality and clinical evidence has showed that air pollution particulate matter(PM) induces the aggravation of AMI.3,4-benzo[a]pyrene(BaP),a polycyclic aromatic hydrocarbon(PAH) with toxicity,is a typical air pollutant present in PM and is often measured as the representative of PAHs.The purpose of this study was to investigate whether BaP can aggravate myocardial infarction(MI) injury and,on this basis,to investigate the relevant mechanisms.The MI mouse model and the oxygen and glucose deprivation(OGD) H9C2 cell model were used to investigate the effect of BaP in MI injury.The involvement of mitophagy and NLRP3-related pyroptosis in regulating deterioration of cardiac function and aggravation of MI injury induced by BaP was comprehensively evaluated.Our study showed that BaP can aggravate MI injury in vivo and in vitro,and this result was based on NLRP3-related pyroptosis induced by BaP.In addition,BaP can inhibit PINK1/Parkin dependent mitophagy through the aryl hydrocarbon receptor(AhR),thus inducing mitochondrial permeability transition pore(mPTP) opening.Our results suggested a role for the BaP from air pollution in the aggravation of MI injury and revealed that BaP aggravates MI injury by activating NLRP3-related pyroptosis through PINK1/Parkin-mitophagy-mPTP opening axis.
Super-strong and flexible wood through cell wall swelling-assisted spontaneously fibers aggregation
Authors: Zhang tao , Chen weimin , Zhang daotong , Chen Yan , Yang kai , Yang pei , Pan nana , Quan qi , Li zhao , Zhou Ke , Chen minzhi , Zhou xiaoyan 
Published: November 2022

Abstract
The highly oriented cellulose microfibrils in secondary cell wall provide a unique advantage for developing super-strength wood. Here, we developed a two-tiered dynamic strategy that realized the self-densified wood by adequate swelling and moisture evaporation process without further hot pressing or via chemical oxidation pre-treatment. Our method requires only exposing delignified wood to a poor solvent that is able to infiltrate the cellulose skeleton without molecular-level dissolution, thus facilitating the effective penetration of moisture upon water replacement. The following natural moisture evaporation triggers self-aggregation of cellulose fibrils accompanying with dynamic re-formation of hydrogen bonds, thereby leading to a super-high mechanical strength (tensile strength: 596.24 ± 57.01 MPa, toughness: 10.43 ± 2.07 MJ m−3, and flexural strength: 418.51 ± 23.86 MPa (balsa wood), 296.52 ± 10.41 MPa (pine wood)) to the resulting self-densified wood. More importantly, the swollen wood has the hydroplastic processing ability (using air-drying to design the shapes). The “cold” processing method satisfies the self-densification for various species of wood, providing a general strengthening strategy.
QoS-Centric Web service Recommendation with Personalized Diversity
Authors: Kang Guosheng , Liang Bowen , Liu Jianxun , Cao Buqing , Xu Yu 
Published: November 2022

Abstract
With the popularity and widespread adoption of SOA (Service Orient Architecture), the number of Web services has increased exponentially on the Internet. Meanwhile, as the cost of Internet applications decreases, users tend to use online Web services for their daily business and software development needs. Under such a background, recommending desirable Web services to users which meet users’ personalized QoS (Quality of Service) requirements becomes a challenging research issue owing to the QoS preference is usually hard to provide for users, i.e., QoS preference is uncertain. To solve the problem, some recent works try to recommend QoS-diversified services to enhance the probability of fulfilling the user’s latent QoS preferences. However, the former QoS-diversified service recommendation methods recommend services with a uniform diversity degree for different users, while the diversified preference requirements are not considered. To this end, this paper proposes to mine a user’s diversity preference from the user’s service invocation history and provides a Web service recommendation algorithm, named personalized DPP (Personalized Determinantal Point Process), through which a personalized service recommendation list with preferred diversity is generated for the user. Comprehensive experiment results show that the proposed approach can provide personalized and diversified Web services on the premise of ensuring the overall accuracy of the recommendation results.
Providing a multi-stage optimization model of vehicle routing and product arrangement in Safir Etka Distribution Company
Authors: sadeghi saeed , Fallah Mohammad , Najafi Seyed Esmaeil 
Published: November 2022

Abstract
This paper presents a vehicle routing model with multiple warehouses and product arrangements in vehicles. Problem modeling consists of two general stages that the result of the first stage directly impacts the decisions of the second stage. Therefore, in the first stage of the problem, the main goal is the optimal arrangement of products requested by customers in packages. While in the second stage, the issue deals with the arrangement of packages (optimal dimensions obtained from the first stage) in vehicles and the optimal routing of vehicles. The goal at this stage is to reduce transportation costs, locate facilities, and select suitable vehicles. Genetic algorithms have been used to optimize the value of the first stage objective function to solve the proposed two-stage model. The weed optimization algorithm has been used to achieve the optimal value of the aim of the second stage function. The result of small sample size problem calculations is the relative difference of 0.68% of the genetic algorithm with GAMS in the first stage and the relative difference of 0.16% of the weed optimization algorithm with GAMS in the second stage; Which shows the high efficiency of the mentioned algorithms in solving the sample problem. Then 10 sample problems were designed and solved in larger sizes. The results show the high efficiency of genetic algorithms and weed optimization in solving large sample problems and case studies in a much shorter time.