bluuebunny/crossref_metadata_2025_split huggingface dataset:
YAP1 controls degeneration of human cartilage chondrocytes in response to mechanical tension
Authors: Ding Baiyang , Xiao Liang , Xu Hongguang 
Published: July 2022

AbstractDisc herniation is a kind of disease caused by degenerative discs, which is common in the elderly, bringing substantial financial burden to families and society. Mechanical tension has a vital effect on the maintenance of cartilage function, however, the molecular mechanism by which mechanical tension causes degenerative discs to remain unclear. This study was the first to reveal Yes‐associated protein 1(YAP1) is a key regulator in mechanical tension‐mediated degenerative discs. Activation of YAP1 may be a valuable strategy to delays the degeneration of human cartilage chondrocytes. We found that YAP1 expression was significantly decreased in degenerative human endplate cartilage and tissue with the strength and time of mechanical stimulation, but the cell cycle distribution was significantly changed under the 10% cyclic mechanical tension(CMT). Besides, the degeneration of endplate cartilage can be delayed by activating the expression level of YAP1 in vitro and it has also been verified in the cartilage endplate tissue in vitro. Furthermore, We found that YAP1 and TEAD1 overexpression increased the activity of the ACAN or COL2A1 promoter to enhance the transcriptional activity of human chondrocyte collagen. The CMT activates the classic Hippo signaling pathway of YAP1, and piezo1 may regulate YAP1 expression through the Hippo signaling pathway. In conclusion, these results suggest the novel mechanism of YAP contributes to delaying the degeneration of endplate cartilage and targeting YAP in combination with Piezo1 is a potential therapeutic approach for the treatment of endplate cartilage degeneration.
Suture connection of overlapping covered CP stents for transcatheter treatment of sinus venosus atrial septal defect with anomalous pulmonary venous connection
Authors: Tan Weiyi , Levi Daniel , Perens Gregory , Aboulhosn Jamil 
Published: July 2022

AbstractTranscatheter correction of a superior sinus venosus defect and partial anomalous pulmonary venous connection with covered stents is a feasible alternative to surgical repair in select patients. Commercially available balloon‐expandable covered stents may be of inadequate length to treat some patients. This may require multiple stents to be placed, which increases the risk of stent migration or embolization. A modification of this technique utilizing two interdigitating covered stents secured together with sutures is described, allowing for increased stability of a long stent complex. One failed case and a second successful case are presented.
The active sealing cuff revolution
Authors: Musumeci Giuseppe , Annibali Gianmarco 
Published: July 2022

Key Points
Among the different transcatheter heart valves available Portico with its dedicated delivery system has demonstrated good results in terms of safety and efficacy.
The Navitor valve is designed with the addition of an active sealing cuff to reduce the degree of PVL and optimize its radial force.
The indication could also expand to low‐ and intermediate‐risk patients and in valve‐in‐valve procedures if these preliminary data are confirmed in subsequent studies
Directional coronary atherectomy revisited
Authors: Grines Cindy L. , Marshall J. Jeffrey 
Published: July 2022

Key Points
Historically, DCA was able to achieve large lumen diameter and reduction in restenosis but at a cost of higher peri‐procedural MI.
Use of a new DCA device in a Japanese registry demonstrated superior acute success and similar in‐hospital complications to PCI without DCA.
DCA may be useful as a niche application in some coronary lesions, but the exact role will need to be defined by additional studies.
Dual guidewire balloon antegrade fenestration and re‐entry technique for coronary chronic total occlusions percutaneous coronary interventions
Authors: Galassi Alfredo R. , Vadalà Giuseppe , Testa Gabriella , Puglisi Sebastiano , Sucato Vincenzo , Diana Davide , Giunta Rocco , Novo Giuseppina 
Published: July 2022

AbstractObjectivesTo describe the experience of coronary chronic total occlusions (CTOs) percutaneous coronary interventions (PCI) using antegrade fenestration and re‐entry (AFR) technique with a dedicated dual guidewire balloon (DGB).BackgroundAntegrade dissection and re‐entry (ADR) techniques has been emphasized in recent worldwide CTO consensus documents. We investigated the feasibility and safety of DGB as a dedicated device to perform guidewire‐based AFR.Methods and ResultsFourteen consecutive patients with complex CTO (J‐CTO score: 3.1 ± 0.9) underwent DGB‐AFR in the years 2020–2021. DGB‐AFR consists in advancing the DGB over a guidewire that reached the vessel distal to the CTO in an extra plaque fashion, inflating/deflating the DGB to create fenestration between subintimal space and the true lumen and advancing a proximal re‐entry guidewire through fenestration in the true lumen. DGB‐AFR alone was successful in 10 of 14 (71%) cases, a rescue wire‐based ADR was needed in two cases for re‐entry into the true lumen with a total success rate in 12 of 14 (86%) cases. Among all DGB‐AFR cases, four (28%) were performed as a first‐line strategy while the remaining 10 (71%) cases were performed as a bail‐out strategy after failure of other antegrade crossings for 30 min of procedural time. No DGB‐related complications were observed.ConclusionsDGB‐AFR is a user‐friendly reliable strategy for the treatment of many CTO lesions. It can be used as bail‐out after failure of conventional antegrade wiring techniques, achieving high procedural success rate and low occurrence of procedural adverse events.
Hydrogen Peroxide‐Responsive Triggers Based on Borinic Acids: Molecular Insights into the Control of Oxidative Rearrangement
Authors: Gatin‐Fraudet Blaise , Pucher Mathilde , Le Saux Thomas , Doisneau Gilles , Bourdreux Yann , Jullien Ludovic , Vauzeilles Boris , Guianvarc'h Dominique , Urban Dominique 
Published: July 2022

AbstractArylborinic acids represent new, efficient, and underexplored hydrogen peroxide‐responsive triggers. In contrast to boronic acids, two concomitant oxidative rearrangements are involved in the complete oxidation of these species, which might represent a major limitation for an efficient effector (drug or fluorophore) release. Herein, a comprehensive study of H2O2‐mediated unsymmetrical arylborinic acid oxidation to investigate the factors that could selectively guide their oxidative rearrangement is described. The o‐CF3 substituent was found to be an excellent directing group allowing a complete regioselectivity on borinic acid models. This result was successfully applied to synthesizing new borinic acid‐based fluorogenic probes, which exclusively release the fluorescent moiety upon H2O2 treatment. These compounds maintained their superior kinetic properties compared to boronic acids, thus further enhancing the potential of arylborinic acids as valuable new H2O2‐sensitive triggers.
Phosphine‐Functionalized Syndiotactic Polystyrenes: Synthesis and Application to Immobilization of Transition Metal Nanoparticle Catalysts
Authors: Jiang Lei , Wang Yinran , Sun Hanyang , Fu Tingting , Hou Zhaomin , Guo Fang 
Published: July 2022

AbstractThe precise control of monomer sequence and stereochemistry in copolymerization is of much interest and importance for the synthesis of high performance materials, but studies toward this goal have met with only limited success to date. The coordination polymerization of diphenylphosphinostyrene (p‐StPPh2 and o‐StPPh2) and its copolymerization with styrene (St) by (C5Me4SiMe3)Sc(CH2C6H4NMe2‐o)2 have been achieved for the first time to afford a new series of phosphine functionalized syndiotactic polystyrene. By the design of the polymer structure, the copolymer of o‐StPPh2 and St (poly(o‐StPPh2‐alt‐St)‐b‐sPS) containing o‐StPPh2 and St atactic alternating copolymer block and syndiotactic polystyrene block (sPS) showed excellent thermal stability and chemical resistance. The simple combination of the triphenylphosphine and syndiotactic polystyrene realized the stable immobilization of metal nanoparticles to afford highly robust metal@poly(o‐StPPh2‐alt‐St)‐b‐sPS nanocatalysts at high temperature and various atmospheres. The Cu@poly(o‐StPPh2‐alt‐St)‐b‐sPS catalyst can serve as a highly efficient heterogeneous catalyst for the synthesis of quinoline derivatives by acceptorless dehydrogenative coupling of o‐aminobenzylalcohol with ketones.
Mechanochemical Bromination of Naphthalene Catalyzed by Zeolites: From Small Scale to Continuous Synthesis
Authors: Ardila‐Fierro Karen J. , Vugrin Leonarda , Halasz Ivan , Palčić Ana , Hernández José G. 
Published: July 2022

AbstractCatalyzed reactions of organic substrates that operate continuously by extrusion techniques are rare. In this study, we developed a mechanochemical bromination of unactivated naphthalene (1) with 1,3‐dibromo‐5,5‐dimethylhydantoin (DBDMH), catalyzed by zeolites in a ball mill. The use ofDBDMHenabled the atom economy of the reaction to be superior compared to the other brominating agents evaluated. Among the zeolites tested, a FAU‐type zeolite demonstrated high catalytic activity and recyclability. The success of the bromination route on a small scale enabled the development of a continuous catalyzed bromination of1by twin‐screw extrusion.
Hydroxide Ion Mechanism for Long‐Range Proton Pumping in the Third Proton Transfer of Bacteriorhodopsin
Authors: Ono Junichi , Okada Chika , Nakai Hiromi 
Published: July 2022

AbstractIn bacteriorhodopsin, representative light‐driven proton pump, five proton transfers yield vectorial active proton translocation, resulting in a proton gradient in microbes. Third proton transfer occurs from Asp96 to the Schiff base on the photocycle, which is expected to be a long‐range proton transfer via the Grotthuss mechanism through internal water molecules. Here, large‐scale quantum molecular dynamics simulations are performed for the third proton transfer, where all the atoms (∼50000 atoms) are treated quantum‐mechanically. The simulations demonstrate that two reaction paths exist along the water wire, namely, via hydronium and via hydroxide ions. The free energy analysis confirms that the path via hydroxide ions is considerably favorable and consistent with the observed lifetime of the transient water wire. Therefore, the proposed hydroxide ion mechanism, as in the first proton transfer, is responsible for the third long‐range proton transfer.
Using Full‐Spectrum Flow Cytometry to Phenotype Memory T and NKT Cell Subsets with Optimized Tissue‐Specific Preparation Protocols
Authors: Farrand Kathryn , Holz Lauren E. , Ferrer‐Font Laura , Wilson Michael D. , Ganley Mitch , Minnell Jordan J. , Tang Ching‐Wen , Painter Gavin F. , Heath William R. , Hermans Ian F. , Burn Olivia K. 
Published: July 2022

AbstractFull‐spectrum flow cytometry is now routinely used in many laboratories internationally, and the demand for this technology is rapidly increasing. With capacity to use larger and more complex staining panels, standardized protocols are required for optimal panel design and analysis. Importantly, for ex vivo analysis, tissue preparation methods also need to be optimized to ensure samples are truly representative of tissues in situ. This is particularly relevant given the recent interest in adaptive immune cells that form residency in specific organs. Here we provide optimized protocols for tissue processing and phenotyping of memory T cells and natural killer T (NKT) cell subsets from liver, lung, spleen, and lymph node using full‐spectrum flow cytometry. We provide a 21‐color antibody panel for identification of different memory subsets, including tissue‐resident memory T (TRM) cells, which are increasingly regarded as important effectors in adaptive immunity. We show that processing procedures can affect outcomes, with liver TRM cells particularly sensitive to heat, such that accurate evaluation requires fast processing at defined temperatures. © 2022 Wiley Periodicals LLC.Basic Protocol 1: Processing mouse liver for flow cytometric analysis of memory T and NKT cell subsetsBasic Protocol 2: Processing mouse spleen for flow cytometric analysis of memory T and NKT cell subsetsBasic Protocol 3: Processing mouse lungs for flow cytometric analysis of memory T and NKT cell subsetsBasic Protocol 4: Processing mouse lymph nodes for flow cytometric analysis of memory T and NKT cell subsetsBasic Protocol 5: Staining and flow cytometric analysis of samples for memory T and NKT cell subsetsSupport Protocol: Obtaining cell counts from flow cytometry data
Development of the CogDrisk tool to assess risk factors for dementia
Authors: Anstey Kaarin J. , Kootar Scherazad , Huque Md Hamidul , Eramudugolla Ranmalee , Peters Ruth 
Published: July 2022

AbstractIntroductionWe aimed to develop a comprehensive risk assessment tool for Alzheimer's disease (AD), vascular dementia (VaD), and any dementia, that will be applicable in high and low resource settings.MethodRisk factors which can easily be assessed in most settings, and their effect sizes, were identified from an umbrella review, or estimated using meta‐analysis where new data were available.ResultsSeventeen risk/protective factors met criteria for the algorithm to estimate risk for any dementia including age, sex, education, hypertension, midlife obesity, midlife high cholesterol, diabetes, insufficient physical activity, depression, traumatic brain injury, atrial fibrillation, smoking, social engagement, cognitive engagement, fish consumption (diet), stroke, and insomnia. A version for AD excluded atrial fibrillation and insomnia due to insufficient evidence and included pesticide exposure. There was insufficient evidence for a VaD risk score.DiscussionValidation of the tool on external datasets is planned. The assessment tool will assist with implementing risk reduction guidelines.
Mid‐aortic syndrome: a rare cause of heart failure in infants
Authors: Zhao Lu , Zhu Li , Zhao Qu‐ming , He Lan , Liang Xue‐cun , Wang Lai‐Shuan , Wu Lin 
Published: July 2022

AbstractThis case reports describe a rare disease, mid‐aortic syndrome (MAS), that can cause severe heart failure and hypertension in infancy. The typical images, key points of diagnosis, and therapy methods of the disease have also been presented. We report two critical thoracoabdominal aortic coarctation cases in infants aged 2 and 11 months with severe heart failure. The patients were initially misdiagnosed as dilated myocardiopathy, with the correct diagnosis confirmed through imaging. Both patients underwent balloon angioplasty; one patient also had bare‐metal stents implanted. The patient treated with balloon angioplasty alone died after the procedure, whereas the other patient recovered well. In conclusion, careful physical examinations, especially upper and lower extremity blood pressure differences and palpation of upper and lower limb pulses, are critical in unexplained infant heart failure cases. Stent implantation may be a safer and more effective treatment than simple balloon angioplasty in infants with MAS.
Host‐Guest Properties of a Trigonal Iminoboronate Ester Cage Self‐Assembled from Hexahydroxytriphenylene
Authors: Herrera‐España Angel D. , Höpfl Herbert , Morales‐Rojas Hugo 
Published: July 2022

AbstractThe self‐assembly of 1,3‐diaminopropane (DAP), 2‐formylphenylboronic acid (FPBA) and 2,3,6,7,10,11‐hexahydroxytriphenylene (HHTP) into a trigonal‐prismatic molecular cage through reversible iminoboronate ester motifs is reported. The organic cage generates solvates with acetonitrile and nitrobenzene, exhibiting vapochromic behavior. Single‐crystal X‐ray diffraction analysis evidenced the inclusion of nitrobenzene within the cage cavity. The resulting host‐guest complex is stabilized by donor‐acceptor‐donor interactions between the nitrobenzene guest and the π electron‐rich HHTP units of the cage.
Novel Chlorination of Imidazo‐Fused Heterocycles via Dichloro(aryl)‐λ3‐iodanes
Authors: Dai Peng‐Fei , Xu Huiqin 
Published: July 2022

AbstractA novel, safe, convenient and efficient protocol has been developed for regioselective chlorination of imidazo‐fused heterocycles with dichloro(4‐(trifluoromethyl)phenyl)‐λ3‐iodanes under mild conditions. The present approach avoids the need of toxic chlorinating reagents, strong stoichiometric oxidants, metal catalysts or harsh reaction conditions. 36 examples of imidazo‐fused heterocycle substrates were investigated, and the chlorinated products were obtained with moderate to excellent yields. Control experiments indicated that the chlorinated reaction proceeded through an electrophilic aromatic substitution.
Flexible biofilm monitoring device
Authors: Reiferth Viktoria Maria , Holtmann Dirk , Müller Daniela 
Published: July 2022

AbstractBiofilms and their analysis are increasingly attracting the attention of the scientific community due to the immense importance and impact of biofilms in various natural, technical and medical fields. For these purposes, an optimized and extended antibiofilm assay system based on the Calgary Biofilm Device (MBEC Assay® system) consisting of microtiter plate and PCR tubes was established. Its implementation was used to study the growth characteristics of the sessile phenotype of Pseudomonas fluorescens exposed to antimicrobial peptides. Inhibitory effects of an antimicrobial peptide on P. fluorescens biofilm formation could be determined at a concentration of 250 μg/ml (biofilm prevention concentration (BPC)) using the modified biofilm assay. Similarly, the biofilm bactericidal concentration (BBC) at 125 μg/ml and the minimum biofilm elimination concentration to remove 90% of the total biofilm mass (MBEC90) were measured at a concentration range of 15.625–1.95 μg/ml. In conclusion, this optimized system provides a highly variable, simple, and cost‐effective alternative to high‐throughput screening based on the Calgary Biofilm Device (CBD).
BER analysis of tightly packed planar RIS system using the level of spatial correlation and discrete phase shifter
Authors: Thirumavalavan Vetrivel Chelian , Ruppa Hariharan Ajay Babu , Thiruvengadam Sundarrajan Jayaraman 
Published: July 2022

AbstractIn this article, the bit error rate (BER) performance of a tightly packed reconfigurable intelligent surface (RIS) assisted communication system is analyzed in terms of the level of spatial correlation. In conventional models, channels are assumed to be independent and identically distributed and continuous phase shifts are available for phase compensation. However, in practice, as RIS reflecting units are arranged in more compact form (less than 0.5) due to space constraints, correlation effects among the reflecting elements are to be considered. Further, discrete phase shifters are widely used to meet practical deployment limitations. These practical constraints severely affect the error performance of the system. It necessitates that the impact of spatial correlation and discrete phase shifts on the BER performance of the system is to be analyzed. The instantaneous signal to noise ratio is the square of the sum of correlated random variables. In order to analyze the BER performance accurately, the exact statistical distribution for the sum of spatially correlated random variables is to be chosen. In this work, appropriate distribution fitting is chosen based on the level of spatial correlation. Numerical BER expressions are derived with the chosen statistical distributions. The analysis on the impact of spatial correlation and discrete phase shifter on BER performance would be more beneficial for design and practicing engineers in the field of 6G wireless communications.
Taxonomic notes onApostasia nipponicaandCrepidium cordilabium(Orchidaceae), two species newly recorded from Hong Kong
Authors: Kumar Pankaj , Gale Stephan 
Published: July 2022

AbstractApostasia nipponicaandCrepidium cordilabiumare newly added to the orchid flora of Hong Kong and their taxonomy is reviewed. A detailed description and line drawing are provided for both species, as are notes on flowering phenology and conservation status. Following careful examination of type material and other authentic specimens from throughout their range, as well as of photographs of plants in the wild,A. nipponicais removed from the synonymy ofA. wallichiiand reinstated as a valid species, under whichA. ramiferaandA. shenzhenicaare newly synonymized. Additionally,Crepidium cordilabium, which was originally described from Taiwan as a natural hybrid betweenC. matsudaeandDienia ophrydis, is treated here as a distinct species belonging to the sectionHololobusand sub‐sectionMaximowiczianae.
Multi‐stage metamorphism of ultrahigh‐temperature Mg‐Al granulites during Gondwana assembly: Evidence from southern India
Authors: Yu Bing , Santosh M. , Tsunogae Toshiaki , Yang Cheng‐Xue , Kim Sung Won 
Published: July 2022

The Southern Granulite Terrane (SGT) in India hosts granulite facies rocks metamorphosed at ultra‐high temperature (UHT) conditions in the various crustal blocks, as well as within the Palghat‐Cauvery Suture Zone (PCSZ), that is considered as a trace of the Late Neoproterozoic—Cambrian Gondwana suture. Here we investigate UHT granulites from the northern margin of the Madurai Block adjacent to the PCSZ where Mg‐Al‐rich granulites are exposed. We identify sodic gedrite + kyanite in these rocks as the high‐pressure prograde stage assemblage, followed by sillimanite‐garnet‐orthopyroxene that formed during pressure decrease and temperature increase. The rare remnant gedrite is also stable at the near‐peak UHT metamorphism until it was replaced by sapphirine. The rocks subsequently underwent decompression that formed sapphirine + cordierite and sapphirine + plagioclase symplectite around sillimanite. Dehydration during decompression generated orthopyroxene‐sillimanite‐quartz assemblage with the appearance of sapphirine, defining the diagnostic mineral assemblage indicative of peak UHT metamorphism (T > 900°C) at relatively high‐pressure (P > 9 kbar). The UHT peak metamorphism in this region is consistent with the results of P–T calculations using conventional geothermometers and phase equilibrium modelling (T up to 1,050°C, P over12 kbar). Zircon and monazite geochronology on the UHT metapelites indicate distinct stages. Detrital zircon grains in the metasediments indicate protolith from ca. 2.5 Ga igneous source and the metamorphic overgrowths yield 206Pb/238U mean ages concentrated at ca. 550–520 Ma. Monazite ages define another younger group 206Pb/238U mean ages at ca. 450 Ma. The prograde high‐pressure granulite‐facies metamorphism and following UHT event correlate with the subduction‐collision tectonics at 550–500 Ma associated with the final stage of amalgamation of the Gondwana supercontinent, while the 420–460 Ma monazite age records a later hydration at the post‐orogenic stage, possibly associated with deep shearing and fluid influx.