bluuebunny/crossref_metadata_2025_split huggingface dataset:
In-depth characterization of denitrifier communities across different soil ecosystems in the tundra
Authors: Pessi Igor S. , Viitamäki Sirja , Virkkala Anna-Maria , Eronen-Rasimus Eeva , Delmont Tom O. , Marushchak Maija E. , Luoto Miska , Hultman Jenni 
Published: June 2022

Abstract
Background
In contrast to earlier assumptions, there is now mounting evidence for the role of tundra soils as important sources of the greenhouse gas nitrous oxide (N2O). However, the microorganisms involved in the cycling of N2O in this system remain largely uncharacterized. Since tundra soils are variable sources and sinks of N2O, we aimed at investigating differences in community structure across different soil ecosystems in the tundra.

Results
We analysed 1.4 Tb of metagenomic data from soils in northern Finland covering a range of ecosystems from dry upland soils to water-logged fens and obtained 796 manually binned and curated metagenome-assembled genomes (MAGs). We then searched for MAGs harbouring genes involved in denitrification, an important process driving N2O emissions. Communities of potential denitrifiers were dominated by microorganisms with truncated denitrification pathways (i.e., lacking one or more denitrification genes) and differed across soil ecosystems. Upland soils showed a strong N2O sink potential and were dominated by members of the Alphaproteobacteria such as Bradyrhizobium and Reyranella. Fens, which had in general net-zero N2O fluxes, had a high abundance of poorly characterized taxa affiliated with the Chloroflexota lineage Ellin6529 and the Acidobacteriota subdivision Gp23.

Conclusions
By coupling an in-depth characterization of microbial communities with in situ measurements of N2O fluxes, our results suggest that the observed spatial patterns of N2O fluxes in the tundra are related to differences in the composition of denitrifier communities.
Feasibility, acceptability, and preliminary effects of PATH FOR timely transfer of geriatric HIP fracture patients from hospital to rehabilitation to home (PATH4HIP): a protocol for a mixed method study
Authors: Backman Chantal , Harley Anne , Papp Steve , Webber Colleen , Poitras Stéphane , Berdusco Randa , Beaulé Paul E. , French-Merkley Veronique 
Published: June 2022

Abstract
Background
Hip fractures in older adults are significant contributors to severe functional decline and disability as well as hospitalization and increased health care costs. Research shows that timely referral to geriatric rehabilitation leads to better patient outcomes. Currently, a wide variability in the timing, the frequency, and the choice of appropriate setting for rehabilitation of hip fracture patients exists.

Aim
Evaluate the feasibility, acceptability, and preliminary effectiveness of PATH4HIP, a pathway intervention for timely transfer of post-operative geriatric hip fracture patients from hospital to rehabilitation to home.

Methods
This is a single-arm, pragmatic feasibility study to measure reach, effectiveness, adoption, implementation, and maintenance of PATH4HIP, a pathway for post-operative hip fracture patients from a large academic health science center to a geriatric rehabilitation service in Ottawa, Canada. During a 6-month period, all hip fracture patients, 65 years of age or older who have undergone surgery and have met the eligibility criteria (n = 96), will be transferred to the geriatric rehabilitation service no later than post-operative day 6. Patients (n = 10–12) and clinicians who are working on the orthopedic team (n = 10–12) and on the geriatric rehabilitation service (n = 10–12) will be invited to participate in an interview to share their feedback on the intervention’s feasibility and acceptability and to provide suggestions to improve PATH4HIP. Descriptive statistics will be used to summarize results of the quantitative data and content analysis will be used to analyze the qualitative data. The study will be open for recruitment from January to July 2022.

Discussion
If feasible, PATH4HIP will result in the reduction of the post-operative acute care length of stay to less than or equal to 6 days, while having no detrimental effect on rehabilitation outcomes such as functional gains, or discharge destination.
The mediating effect of knowledge sharing in the relationship between factors affecting knowledge sharing and reflective thinking: the case of English literature students during the COVID-19 crisis
Authors: Farahian Majid , Parhamnia Farshad , Maleki Nasser 
Published: June 2022

AbstractThe COVID-19 pandemic has had serious implications on educational systems worldwide and, hence, online courses have been organized. It is expected that the use of online learning in higher education promote knowledge sharing among students and the sharing of knowledge result in the improvement of reflective thinking among them. As such, we examined the knowledge sharing behavior among the undergraduate students in online learning English literature courses, the student’ perceptions towards reflective thinking, the relationship between the students’ knowledge sharing and reflective thinking, and finally, we tested a structural model of factors affecting knowledge sharing components, knowledge sharing, and reflective thinking. The data were collected through two surveys of 104 Iranian English literature students. A Pearson’s correlation coefficient and path analysis were used to analyze the data and to generate a model. The results showed that the students’ online knowledge sharing behavior and their perceptions towards reflective thinking are at unacceptable levels. Furthermore, a significant relationship was found between factors affecting knowledge sharing with the students’ knowledge sharing behavior, and between knowledge sharing and reflective thinking. The results also confirmed the mediator role of knowledge sharing and supported the hypothesized model of the relationships among the variables. Pedagogical implications of the study are finally discussed.
Assessing facilitating conditions and barriers for innovation implementation in Canadian long-term care homes: a research protocol
Authors: Hardy Marie-Soleil , Sasseville Maxime , Attieh Randa , Bergeron-Drolet Laurie-Ann , Sanchez Romina Helena Barony , Gallani Maria Cecilia , Côté André , Laberge Maude , Voyer Philippe , Couture Vincent , Gagnon Marie-Pierre 
Published: June 2022

Abstract
Background
The COVID-19 pandemic has profoundly affected the health and care of older adults, with particularly negative consequences for those residing in long-term care homes (LTCH) and retirement homes (RH). To inform the implementation of interventions with the most potential for impact, Healthcare Excellence Canada identified six promising practices and policy options that can be introduced to ensure that LTCH and RH are better prepared for potential future outbreaks. A total of 22 implementation science teams (ISTs) were funded to support LTCH and RH across Canada in their implementation of these practices. This study aims to identify the enablers and barriers to the successful implementation of evidence-based practices and the impact of intervention in LTCH and RH across Canada.

Methods
A survey-based longitudinal correlational design will be used. The Organizational Readiness for Knowledge Translation (OR4KT) tool will be used to assess the readiness of LTCH and RH to implement the selected practice. The OR4KT includes 59 questions and takes about 15 min to complete. Five to ten respondents per organization, holding different job positions, will be invited by the ISTs to complete the OR4KT in 91 LTCH or RH across Canada at the beginning of the project (T1) and 6 months after the first measurement (T2).

Discussion
The study will provide a benchmark for assessing the readiness of LTCH and RH to implement evidence-based practices. It will also inform decision-makers about barriers and facilitators that influence the integration of promising practices in these organizations.
Implementing specialised vestibular physiotherapy in an emergency department: a process evaluation
Authors: Ip Kelvin , Lloyd Melanie , Luscombe Allison , Hitch Danielle 
Published: June 2022

Abstract
Background
Dizziness and vertigo-like symptoms, often caused by common peripheral vestibular disorders such as benign paroxysmal positional vertigo (BPPV), may significantly impact function and quality of life. These symptoms often result in emergency department (ED) presentations. Evidence-based clinical practice guidelines strongly recommend using physical assessment and treatment manoeuvres for the assessment, diagnosis and treatment of these symptoms. This study aimed to evaluate the process of implementing specialised vestibular physiotherapy (SPV) in an emergency department from the clinician’s perspective.

Methods
This implementation study utilised a retrospective mixed-methods process evaluation to understand how SVP operated in an Australian emergency department. The i-PARiHS framework was embedded within the methodology and analytical approach of the study to ensure a comprehensive approach closely aligned to implementation science. Nine clinicians retrospectively completed the Organisational Readiness for Change Assessment (ORCA), Acceptability of Intervention Measure (AIM), Intervention Appropriateness Measure (IAM) and Feasibility of Intervention Measure (FIM). Seven clinicians also participated in a focus group or interview.

Results
A range of barriers and facilitators to the implementation process were identified by participants, some of which spanned multiple domains of the i-PARiHS framework. Relationships with service leaders, champions and medical staff were pivotal facilitators to implementation, along with a generally held perception that SVP was acceptable and feasible. The main barrier identified was a lack of capacity to deliver and facilitate this innovation within the physiotherapy workforce and the broader multidisciplinary recipients.

Conclusions
This study demonstrates that the process of implementing an SVP service in an ED context was generally well-received by clinicians but also involved some challenges and barriers. Services looking to implement SVP in the ED should aim to build stakeholder relationships; develop a shared vision with clear goals and intended outcomes; embed the innovation in organisation processes, procedures and policies; and increase workforce capacity to deliver and facilitate SVP to guide their approach to this innovation.
Optimized rice transformation protocol for transformation of the blast susceptible Indica rice accession CO39
Authors: Greenwood Julian R. , Glaus Anna N. 
Published: June 2022

Abstract
Background
Many rice transformation protocols have been reported, but optimization is still required to ensure efficient transformation of specific rice accessions. The modified rice transformation protocol presented here builds upon the original protocol: ‘An improved protocol for efficient transformation and regeneration of diverse Indica rice cultivars’ volume 7, Article number: 49 (2011), Plant Methods.

Results
Following the aforementioned transformation protocol, calli browning occurred and no Agrobacterium-mediated transformation could be achieved, but this could be remedied by increasing the concentration of l-Proline. Improved callus health lead to successful transformation and proliferation of calli on selection media, but a low frequency of plantlet regeneration occurred when calli were transferred to regeneration media. The efficiency of plantlet regeneration was greatly improved by removing antibiotics from regeneration media, with the presence of escapes selected against during subsequent transfer of plantlets to antibiotic containing rooting media. Transformation of CO39 callus was found to be possible 8 days after callus induction resulting in a time saving of 10 days compared to the original protocol.

Conclusions
This optimized transformation protocol allows for the generation and survival of healthy CO39 calli, efficient transformation of calli using Agrobacterium, and produces a high frequency of regenerated transgenic plants. These protocol modifications will be useful for optimizing the transformation and regeneration of other recalcitrant Indica rice cultivars, particularly those sensitive to antibiotics during plantlet regeneration.
The potential for gene-editing to increase muscle growth in pigs: experiences with editing myostatin
Authors: Dilger A. C. , Chen X. , Honegger L. T. , Marron B. M. , Beever J. E. 
Published: June 2022

AbstractGene-editing holds promise as a new technique for growth promotion in livestock, especially in the face of increased opposition to traditional methods of growth promotion like feed additives. However, to date, there has been only limited progress toward models of growth promotion through gene-editing. The vast majority of gene-editing projects have focused on a single gene, myostatin (MSTN), with several reports of successful editing events. These attempts have been limited by the low efficiency of successful edits and issues of viability. The use of both microinjection and somatic cell nuclear transfer appear to be susceptible to these viability issues. Herein, we report a successful editing of myostatin in pigs using a zinc-finger nuclease targeted at exon 3 of myostatin. Overall, the successful editing rate was 1% (37 edited cell lines/3616 screened cell lines). Edits included a variety of small indels and larger deletions. One male and one female cell line with a deletion of one thymidine residue (− 1 T) were selected for somatic cell nuclear transfer. Of the ~ 900 embryos transferred, there were 12 live births (1 male, 11 females) but only 5 female pigs survived to sexual maturity. These animals were bred to commercial sires to expand the population and determine potential interactive effects between myostatin mutations and the naturally-occurring g.3072G > A mutation in insulin-like growth factor 2. Even in subsequent generations (F0xWT, F1xF0, F1xF1), viability of piglets continued to be poor and was associated with the progeny’s proportion of the original clone genome, even in pigs lacking MSTN loss-of-function (LOF) mutations. However, viability of pigs with myostatin LOF (MSTN−/−) was especially poor as none of the 37 animals born were viable. Sequencing of cloned pigs indicated that off-target effects did not explain this poor viability. Reducing the percentage of the cloned genome by outcrossing successfully improved viability of MSTN+/+ and MSTN± pigs, but not of MSTN−/− pigs. Characterization of MSTN± pigs did reveal an increase in muscle growth and body weight compared with MSTN+/+ pigs. Therefore, in order for the promise of gene-editing of myostatin for growth promotion in livestock to be fulfilled, issues of viability of offspring and efficacy of editing have yet to be overcome. Additionally, gene-editing targets other than myostatin must be explored.
Directly wireless communication of human minds via non-invasive brain-computer-metasurface platform
Authors: Ma Qian , Gao Wei , Xiao Qiang , Ding Lingsong , Gao Tianyi , Zhou Yajun , Gao Xinxin , Yan Tao , Liu Che , Gu Ze , Kong Xianghong , Abbasi Qammer H. , Li Lianlin , Qiu Cheng-Wei , Li Yuanqing , Cui Tie Jun 
Published: June 2022

AbstractBrain-computer interfaces (BCIs), invasive or non-invasive, have projected unparalleled vision and promise for assisting patients in need to better their interaction with the surroundings. Inspired by the BCI-based rehabilitation technologies for nerve-system impairments and amputation, we propose an electromagnetic brain-computer-metasurface (EBCM) paradigm, regulated by human’s cognition by brain signals directly and non-invasively. We experimentally show that our EBCM platform can translate human’s mind from evoked potentials of P300-based electroencephalography to digital coding information in the electromagnetic domain non-invasively, which can be further processed and transported by an information metasurface in automated and wireless fashions. Directly wireless communications of the human minds are performed between two EBCM operators with accurate text transmissions. Moreover, several other proof-of-concept mind-control schemes are presented using the same EBCM platform, exhibiting flexibly-customized capabilities of information processing and synthesis like visual-beam scanning, wave modulations, and pattern encoding.
A Novel Germline Deletion of p.C630 inRETCauses MTC and Promotes Cell Proliferation and Sensitivity to Pralsetinib
Authors: Ma Xiao , Ma Xiuli , Chin Lihan , Zhu Zhen , Han Haibo 
Published: June 2022

AbstractContextMedullary thyroid cancer (MTC) is usually caused by gain-of-function mutations in the proto-oncogene RET.ObjectiveThis study aimed to determine the underlying mechanism in a male patient diagnosed with MTC at age 51 years.MethodsGenomic DNA extracted from leukocytes or tumor tissues of patients was used for next-generation sequencing (NGS)-panel sequencing and Sanger sequencing. Wild-type (WT) and p.C630 deletion RET were expressed in HEK 293T cells. Activation of phosphorylation of the crucial tyrosine-905 of RET and MAPK/ERK was analyzed by Western blotting. The effect of RET mutants on cell viability and colony formation ability was determined by CCK8 assay and a colony forming assay.ResultsNGS-Panel sequencing revealed a 3-nucleotide/1-amino acid C630 in-frame deletion in exon 11 of RET (c.1887_1889delGTG p.C630del). In vitro expression showed that phosphorylation of the crucial tyrosine 905 was much stronger in the p.C630del RET mutant than in WT RET, indicating ligand-independent activation of the Ret protein tyrosine kinase. Furthermore, p.C630del RET mutant induced strong activation of the MAPK/ERK pathway. In addition, p.C630del RET mutant cells exhibited increased HEK 293T cell viability and colony formation compared with WT RET cells. Pralsetinib (BLU-667), a highly selective RET inhibitor, inhibited the viability of WT RET and p.C630del RET mutant-transfected HEK 293T cells (IC50s: 18.54 and 16.49 µM after treatment for 24 hours), followed by inhibition of the RET-induced MAPK/ERK pathway.ConclusionThe finding in our patient with MTC was a 3-base-pair deletion in exon 11 of RET, a p.C630 deletion not previously reported. The p.C630del RET stimulates cell proliferation by increasing ligand-independent phosphorylation and activation of MAPK/ERK pathway, demonstrating the pathogenic nature of the mutation. We therefore recommend screening panel sequence of RET in MTC patients with indications of a genetic cause.
Existence and concentration of ground-states for fractional Choquard equation with indefinite potential
Authors: Zhang Wen , Yuan Shuai , Wen Lixi 
Published: June 2022

AbstractThis paper is concerned with existence and concentration properties of ground-state solutions to the following fractional Choquard equation with indefinite potential:(−Δ)su+V(x)u=∫RNA(εy)∣u(y)∣p∣x−y∣μdyA(εx)∣u(x)∣p−2u(x),x∈RN,{\left(-\Delta )}^{s}u+V\left(x)u=\left(\mathop{\int }\limits_{{{\mathbb{R}}}^{N}}\frac{A\left(\varepsilon y)| u(y){| }^{p}}{| x-y{| }^{\mu }}{\rm{d}}y\right)A\left(\varepsilon x)| u\left(x){| }^{p-2}u\left(x),\hspace{1em}x\in {{\mathbb{R}}}^{N},wheres∈(0,1)s\in \left(0,1),N>2sN\gt 2s,0<μ<2s0\lt \mu \lt 2s,2<p<2N−2μN−2s2\lt p\lt \frac{2N-2\mu }{N-2s}, andε\varepsilonis a positive parameter. Under some natural hypotheses on the potentialsVVandAA, using the generalized Nehari manifold method, we obtain the existence of ground-state solutions. Moreover, we investigate the concentration behavior of ground-state solutions that concentrate at global maximum points ofAAasε→0\varepsilon \to 0.
Hemothorax after fetal pleural effusion-thoracoamniotic shunting procedure due to transient myeloproliferative disorder
Authors: Iwahata Yuriko , Iwahata Hideyuki , Hasegawa Junichi , Homma Chika , Nishimura Yoko , Kondo Haruhiro , Suzuki Nao 
Published: June 2022

Abstract

Objectives
To present a case of fetal hemothorax after two times of thoracoamniotic shunting (TAS) performed to pleural effusion with hydrops fetalis, resulting in non-reassuring fetal status due to hemothorax.

Case presentation
This is a case of bilateral pleural effusion with hydrops fetalis and polyhydramnios at 32 weeks gestation, in which unilateral fetal TAS was performed twice, resulting in non-reassuring fetal status due to hemothorax. After delivery, the infant was diagnosed with trisomy 21 and transient myeloproliferative disorder (TMD) with disseminated intravascular coagulation and congenital systemic lymphangiopathy.

Conclusions
In conclusion, since TAM case do not always show hemothrax, TAM is not inhibited but technical carefulness should be necessary.
The simulation of two-dimensional plane problems using ordinary state-based peridynamics
Authors: Zhao Jingjing , Lu Guangda , Zhang Qing , Du Wenchao 
Published: June 2022

Abstract
The ordinary state-based peridynamics (OSB PD) model is an integral nonlocal continuum mechanics model. And the three-dimensional OSB PD model can deal with linear elastic solid problems well. But for plane problems, the calculation results of existing models have large deviations. In this paper, a set of OSB PD models for plane problems is established by theoretical derivation. First, through the strain energy density function equivalence of peridynamics and classical continuum mechanics, the equivalent coefficients of the plane strain and plane stress problems of OSB PD are deduced. Then, consider the cantilever beam deformation simulation under concentrated load. The simulation results show that the maximum displacements are in good agreement with the corresponding analytical solutions in all directions. Finally, in the simulation of the slab with a hole, the two cases of uniform displacement and uniform load are considered, respectively. The simulation results are consistent with the ANSYS analysis results, and the deviation is small, which verifies the validity of the model.
Effect of Om chanting and Yoga Nidra on depression anxiety stress, sleep quality and autonomic functions of hypertensive subjects – a randomized controlled trial
Authors: Rajagopalan Archana , Krishna Anjana , Mukkadan Joseph Kurian 
Published: June 2022

Abstract

Introduction
Hypertension (HTN) is a common and growing public health challenge with severe risk factors. Hence, this study aimed to assess the effect of Om chanting and Yoga Nidra on depression, anxiety, stress, sleep quality and autonomic functions on individuals with hypertension.

Methods
This prospective randomized controlled study was conducted in patients with hypertension at Little Flower Medical Research Center. A total of 80 patients with diagnosed hypertension were recruited and randomized equally to either the experimental group or control group. The experimental group received a combination of Om chanting and Yoga Nidra for five days a week for two months. The control group participants continued with their regular conventional medications. Depression anxiety stress scale (DASS), Pittsburgh sleep quality index (PSQI) and heart rate variability (HRV) scores were assessed at baseline, 30 and 60 day for both the groups.

Results
A total of 34 subjects in the experimental group and 31 subjects in the control group were included in the analysis. There was a significant (p<0.001) reduction in depression, anxiety, stress, and a significant (p<0.001) improvement in PSQI and HRV parameters in the experimental group was observed as compared to the control group. No adverse events were reported during the trial period.

Conclusions
The current study validates the effectiveness of Om chanting and Yoga Nidra in reducing depression, anxiety, stress and improving sleep quality and autonomic functions in hypertensive patients. These interventions could thus be considered a safer form of complementary therapy in managing stress and hypertension.