bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA GAS5 regulates the Wnt/β-catenin pathway through the miR-18a-5p/AXIN2/GSK3β axis to inhibit the proliferation and migration of bladder cancer cells
Authors: Zhang Ze , Liu Tiantian , Cheng Chao , Wang Jiawei , Wang Chong , Huang Houbao , Li Yawei 
Published: November 2022

Abstract
LncRNA growth arrest specific 5 (GAS5) has been confirmed to play an essential role in a number of biological processes, such as tumor regulation and gene transcription. GAS5 has been shown to be a tumor suppressor gene in many types of cancer, but its specific mechanism of action in bladder cancer (BC) remains to be elucidated. In this study, we explored the biological properties of GAS5 in BC and its mechanism of action in BC. We analyzed the expression of GAS5 in 50 pairs of BC tissues and found that GAS5 was low expressed in BC tissues compared with normal mucosal tissues. In vitro and in vivo experiments showed that GAS5 could affect the proliferation and migration of BC cells. Nucleoplasmic isolation assays and fluorescence in situ hybridization (FISH) assays demonstrated the localization of GAS5 in cell cytoplasm. Chromatin isolation by RNA purification (ChIRP), RNA immunoprecipitation (RIP) and luciferase assay demonstrated the target binding relationship of GAS5 with miR-18a-5p. Rescue experiments demonstrated that GAS5 promoted the proliferation and migration of BC cells through target binding of miR-18a-5p. Moreover, miR-18a-5p bound to its targets AXIN2 and GSK3β, which in turn affected the expression of Wnt/β-catenin pathway-related proteins. Our findings demonstrate that GAS5 regulates Wnt/β-catenin pathway activity by regulating the miR-18a-5p/AXIN2/GSK3β axis to modulate BC progression, providing a new potential therapeutic strategy for the treatment of BC.
Health-related quality-of-life up to one year after myocardial infarction with non-obstructive coronary arteries
Authors: Berg Emma , Agewall Stefan , Brolin Elin B , Caidahl Kenneth , Cederlund Kerstin , Collste Olov , Daniel Maria , Ekenbäck Christina , Jensen Jens , Y-Hassan Shams , Henareh Loghman , Maret Eva , Spaak Jonas , Sörensson Peder , Tornvall Per , Lyngå Patrik 
Published: November 2022

Abstract

Aims
Patients with Myocardial Infarction with Non-Obstructive Coronary Arteries (MINOCA) are a heterogenous group and previous studies indicate a decreased Health-related quality-of-life (HRQoL) compared with patients with myocardial infarction with obstructive coronary artery disease and healthy individuals. However, longitudinal data are scarce. Therefore, the aim was to explore HRQoL among patients with MINOCA during a one-year period after the acute event in comparison with a group of healthy individuals and to describe HRQoL in patients with Takotsubo Syndrome (TTS).

Methods and results
Patients with MINOCA were recruited from five hospitals in the Stockholm region (SMINC-2 study, clinical trials: NCT2318498). Patients responded to the HRQoL questionnaire RAND-36 between days 2–4, after 6 and 12 months respectively. A sample of population-based individuals was used as a comparison group. A total of 142 MINOCA patients, (70% women) mean age of 56 years, responded. A population-based sample of 317 volunteers (66% women) mean age of 57 years. Patients with MINOCA scored lower than the comparison group in the domains role functioning physical, social functioning, and role functioning emotional (P = 0.01–0.02) at 12 months. In these domains of HRQoL there was no improvement in MINOCA patients during 12 months follow-up. In the domains of energy/fatigue vitality and emotional well-being the scores improved and were similar to the comparison group at 12 months. Patients with TTS scored generally lower on RAND-36 than MINOCA patients without TTS.

Conclusion
Physical, social, and emotional functioning did not improve during the first year after MINOCA, indicating a need for increased follow-up including psychological support.
Inclisiran and cardiovascular events: a patient-level analysis of phase III trials
Authors: Ray Kausik K , Raal Frederick J , Kallend David G , Jaros Mark J , Koenig Wolfgang , Leiter Lawrence A , Landmesser Ulf , Schwartz Gregory G , Lawrence David , Friedman Andrew , Garcia Conde Lorena , Wright R Scott ,   ORION Phase III investigators
Published: November 2022

Abstract

Background
Inclisiran, an siRNA administered twice-yearly, significantly reduced LDL cholesterol (LDL-C) in Phase III trials. Whether lowering LDL-C with inclisiran translates into a lower risk of cardiovascular (CV) events is not yet established.

Methods and results
Patient-level, pooled analysis of ORION-9, −10 and −11, included patients with heterozygous familial hypercholesterolaemia, atherosclerotic CV disease (ASCVD), or ASCVD risk equivalent on maximally tolerated statin-therapy, randomized 1:1 to receive 284 mg inclisiran or placebo on Days 1, 90, and 6-monthly thereafter for 18 months. Prespecified exploratory endpoint of major cardiovascular events (MACEs) included non-adjudicated CV death, cardiac arrest, non-fatal myocardial infarction (MI), and fatal and non-fatal stroke, evaluated as part of safety assessments using a standard Medical Dictionary for Regulatory Activities basket. Although not prespecified, total fatal and non-fatal MI, and stroke were also evaluated. Mean LDL-C at baseline was 2.88 mmol/L. At Day 90, the placebo-corrected percentage reduction in LDL-C with inclisiran was 50.6%, corresponding to an absolute reduction of 1.37 mmol/L (both P &lt; 0.0001). Among 3655 patients over 18 months, 303 (8.3%) experienced MACE, including 74 (2.0%) fatal and non-fatal MIs, and 28 (0.8%) fatal and non-fatal strokes. Inclisiran significantly reduced composite MACE [OR (95% CI): 0.74 (0.58–0.94)], but not fatal and non-fatal MIs [OR (95% CI): 0.80 (0.50–1.27)] or fatal and non-fatal stroke [OR (95% CI): 0.86 (0.41–1.81)].

Conclusion
This analysis offers early insights into the potential CV benefits of lowering LDL-C with inclisiran and suggests potential benefits for MACE reduction. These findings await confirmation in the larger CV outcomes trials of longer duration.
Serial troponin-T and long-term outcomes in suspected acute coronary syndrome
Authors: Pareek Manan , Kragholm Kristian H , Kristensen Anna Meta Dyrvig , Vaduganathan Muthiah , Pallisgaard Jannik L , Byrne Christina , Biering-Sørensen Tor , Lee Christina Ji-Young , Bonde Anders Nissen , Mortensen Martin Bødtker , Maeng Michael , Fosbøl Emil L , Køber Lars , Olsen Niels Thue , Gislason Gunnar H , Bhatt Deepak L , Torp-Pedersen Christian 
Published: November 2022

Abstract

Background
Long-term prognostic implications of serial high-sensitivity troponin concentrations in subjects with suspected acute coronary syndrome are unknown.

Methods and results
Individuals with a first diagnosis of myocardial infarction, unstable angina, observation for suspected myocardial infarction, or chest pain from 2012 through 2019 who underwent two high-sensitivity troponin-T (hsTnT) measurements 1–7 h apart were identified through Danish national registries. Absolute and relative risks for death at days 0–30 and 31–365, stratified for whether subjects had normal or elevated hsTnT concentrations, and whether these concentrations changed by &lt;20%, &gt; 20 to 50%, or &gt;50% in either direction from first to second measurement, were calculated through multivariable logistic regression with average treatment effect modeling. Of the 28 902 individuals included, 2.8% had died at 30 days, whereas 4.9% of those who had survived the first 30 days died between days 31–365. The standardized risk of death was highest among subjects with two elevated hsTnT concentrations (0–30 days: 4.3%, 31–365 days: 7.2%). In this group, mortality was significantly higher in those with a &gt; 20 to 50% or &gt;50% rise from first to second measurement, though only at 30 days. The risk of death was very low in subjects with two normal hsTnT concentrations (0–30 days: 0.1%, 31–365 days: 0.9%) and did not depend on relative or absolute changes between measurements.

Conclusions
Individuals with suspected acute coronary syndrome and two consecutively elevated hsTnT concentrations consistently had the highest risk of death. Mortality was very low in subjects with two normal hsTnT concentrations, irrespective of changes between measurements.
Country differences in the cross-sectional associations between smoking and depressive symptoms in adolescence
Authors: Raffetti Elena , Donato Francesco , Triolo Federico , Andersson Filip , Forsell Yvonne , Galanti Maria Rosaria 
Published: November 2022

Abstract

Background
The aim of the present study was to compare the cross-sectional association between smoking and depressive symptoms among adolescents between Sweden and Italy, two countries historically characterized by different norms about tobacco use and different tobacco control efforts.

Methods
A cross-sectional study including 3283 adolescents 15–16 years of age participating in the Swedish KUPOL study and 1947 same-age adolescents from the Italian BE-TEEN study. Current smoking was defined as any smoking in the past 30 days. Depressive symptoms were assessed using the Centre for Epidemiological Studies Depression Scale for Children (CES-DC) and the internalizing score of the Strengths and Difficulties Questionnaire (SDQ). Country differences were explored in stratified and interaction analyses.

Results
Current smoking was associated with a 2- to 3-fold increased odds of depressive symptoms among Swedish adolescents using both CES-DC and SDQ internalizing scale. Among Italian adolescents, slightly lower increased odds of 1.5–2.5 for depressive symptoms with smoking were found using the CES-DC but not the SDQ scale. Both multiplicative and additive interactions for country were significant. The association between smoking and depressive symptoms was weaker among Italian compared with Swedish adolescents for both scores.

Conclusions
Countries with different tobacco norms and control show different associations between smoking and depressive symptoms in adolescence, probably due to different psychosocial profiles of smokers. These findings need to be considered when planning tobacco prevention programmes, e.g. by focusing on early detection of mental health distress among adolescents in settings with declining smoking prevalence and restrictive tobacco control environments.
Alignstein: Optimal transport for improved LC-MS retention time alignment
Authors: Skoraczyński Grzegorz , Gambin Anna , Miasojedow Błażej 
Published: November 2022

Abstract

Background
Reproducibility of liquid chromatography separation is limited by retention time drift. As a result, measured signals lack correspondence over replicates of the liquid chromatography–mass spectrometry (LC-MS) experiments. Correction of these errors is named retention time alignment and needs to be performed before further quantitative analysis. Despite the availability of numerous alignment algorithms, their accuracy is limited (e.g., for retention time drift that swaps analytes’ elution order).

Results
We present the Alignstein, an algorithm for LC-MS retention time alignment. It correctly finds correspondence even for swapped signals. To achieve this, we implemented the generalization of the Wasserstein distance to compare multidimensional features without any reduction of the information or dimension of the analyzed data. Moreover, Alignstein by design requires neither a reference sample nor prior signal identification. We validate the algorithm on publicly available benchmark datasets obtaining competitive results. Finally, we show that it can detect the information contained in the tandem mass spectrum by the spatial properties of chromatograms.

Conclusions
We show that the use of optimal transport effectively overcomes the limitations of existing algorithms for statistical analysis of mass spectrometry datasets. The algorithm’s source code is available at https://github.com/grzsko/Alignstein.
Topography effect on ambient noise tomography: a case study for the Longmen Shan area, eastern Tibetan Plateau
Authors: Jin Ruizhi , He Xiaohui , Fang Hongjian , Xie Jun , Liu Ying , Zhang Peizhen 
Published: November 2022

SUMMARY
Ambient noise tomography (ANT) is a widely used method to obtain shear wave velocity structure in the crust and upper mantle. Usually, the topography is assumed to have negligible effect on the resulting models. This, however, might not be proper in regions with large topographic variation, such as plateau edges, submarine slopes and volcanic islands. In this study, we use synthetics from waveform-based numerical simulation to quantify the topography effect on ANT in the Longmen Shan area, eastern Tibetan Plateau margin. Three kinds of models are used in forward simulation to obtain theoretical waveforms, including Case1: the layered model, Case2: the layered model with topographic variation and Case3: the flattened model of Case2. The final inversion results show that the bias of ANT is negligible in the blocks with relatively flat topography, such as the interior regions of the Tibetan Plateau and the Sichuan Basin. However, for the Longmen Shan boundary zone with significant topographic variation (∼4 km), the shear wave velocity image has an obvious negative bias that can reach up to −4 per cent. The maximum depth of bias is ∼5 km, which is mirrored with the maximum topographic elevation difference of the region, and the average bias disappears as the depth decreases to the surface (0 km) or increases to three times of the maximum influence depth (∼15 km). The horizontal distribution of the tomographic bias is almost linearly related to the topographic elevation difference with a slope of −1.04 and a correlation coefficient of 0.90 at maximum influence depth. According to this first-order correction formula and the decreasing trend of average bias with depth, the topography effect on ANT can be suppressed to a certain extent.
The policy environment of self-care: a case study of the Philippines
Authors: Jaminola Leonardo Iii , Negre Jana Marie , Pepito Veincent Christian F , Loreche Arianna Maever , Dayrit Manuel M 
Published: November 2022

Abstract
Self-care is the ability and empowerment of individuals to maintain health through informed health-care decisions, with or without the support of a health provider. High-income countries have made advances to their conceptualization, research and institutionalization of self-care, given its reported benefits to patients, the health system and economy. A similar undertaking in low- and middle-income countries (LMICs) with already fragile health systems is warranted as highlighted by the coronavirus disease 2019 pandemic. Our article therefore aimed to describe and analyse the policy environment of self-care using the Philippines as a case study, which may have relevance to other similar countries and settings that are transitioning towards Universal Health Care (UHC) to reform and strengthen their primary care systems. We conducted 13 key informant interviews and 2 focus group discussions among representatives from the government, the pharmaceutical retail/industry, community retail pharmacy, primary health physicians and health workers, an infirmary administrator and patients and/or patient advocates. We triangulated our qualitative data with findings from our policy review. We found a total of 13 relevant policies on self-care in the Philippines recently drafted and/or implemented from 2016 to 2021 that fall under the broad categories of unifying frameworks and road maps, capacity building and institutional streamlining, regulations and disease guidelines. Our case study highlights the role of the UHC Law as a driver for self-care and patient empowerment towards better health outcomes with its passage resulting in the promulgation of self-care-related policies. Our findings also suggest that changes in the local policy and built environment, and the formal educational and health systems, are needed to foster a culture of responsible self-care. There are notable exemplars in advancing self-care in the region, including Thailand, from which LMICs like the Philippines can draw lessons to make progress on institutionalizing self-care and, ultimately, realizing UHC and Health For All.
Population dynamics and gene regulation of T cells in response to chronic antigen stimulation
Authors: Hsiung Sunnie , Egawa Takeshi 
Published: November 2022

Abstract
T cells are activated by antigen and co-stimulatory receptor signaling and undergo robust proliferation and differentiation into effector cells with protective function. Such quantitatively and qualitatively amplified T cell responses are effective in controlling acute infection and are followed by contraction of the effector population and the formation of resting memory T cells for enhanced protection against previously experienced antigens. However, in the face of persistent antigen during chronic viral infection, in autoimmunity, or in the tumor microenvironment, T cells exhibit distinct responses relative to those in acute insult in several aspects, including reduced clonal expansion and impaired effector function associated with inhibitory receptor expression, a state known as exhaustion. Nevertheless, their responses to chronic infection and tumors are sustained through the establishment of hierarchical heterogeneity, which preserves the duration of the response by generating newly differentiated effector cells. In this review, we highlight recent findings on distinct dynamics of T cell responses under “exhausting” conditions and the roles of the transcription factors that support attenuated yet long-lasting T cell responses as well as the establishment of dysfunctional states.
Will the United States come to Taiwan’s defense? Analysis of public opinion in Taiwan and the United States
Authors: Wu Chung-li , Lin Alex Min-Wei 
Published: November 2022

Abstract
If conflict breaks out between Taiwan and China, would the United States come to the island’s defense? China’s growing assertiveness in the region and aggressive military activities around Taiwan have renewed interest in this decades-old question. This study examines the issue by employing two surveys, one conducted in Taiwan and another in the United States. Results of the Taiwan survey indicate that while a majority of Taiwan citizens do indeed believe the United States would help defend Taiwan if it were attacked by China, five types of respondents—idealists, pragmatists, democracy skeptics, political realists, and pessimists—can be discerned, each with a distinctive pattern of reasoning. Findings from the US survey reveal that while Americans mostly hold positive views of Taiwan, there is little consensus on the preferred US military response in the event of a Chinese attack. These analyses contribute to the existing literature on evolving public opinion in Taiwan and the United States concerning the possibility of armed conflict in the Taiwan Strait.
What a 31-yr multibreed herd taught us about the influence of B. indicus genetics on reproductive performance of cows
Authors: Martins Thiago , Rocha Cecilia C , Driver Joseph Danny , Rae Owen , Elzo Mauricio A , Mateescu Raluca G , Santos Jose Eduardo P , Binelli Mario 
Published: November 2022

Abstract
Bos taurus × Bos indicus crosses are widespread in tropical and subtropical regions, nonetheless, quantitative information about the influence of B. indicus genetics on the reproductive performance of beef cattle is lacking. Herein, we determined the association between level of B. indicus genetics and reproduction from a 31-yr dataset comprising sequential breeding seasons of the University of Florida multibreed herd (n = 6,503 Angus × Brahman cows). The proportion of B. indicus genetics in this herd is evenly distributed by each 1/32nd or approximately 3-percentage points. From 1989 to 2020, the estrous cycle of cows was synchronized for artificial insemination (AI) based on detected estrus or timed-AI (TAI) using programs based on gonadotropin-releasing hormone and prostaglandin, and progestin/progesterone. All cows were exposed to natural service after AI and approximately 90-d breeding seasons, considering the day of AI as day 0. The proportion of B. indicus genetics of cows was associated negatively with pregnancy per AI, ranging from 51.6% for cows with 0%–19% of B. indicus genetics to 37.4% for cows with 81%–100% of B. indicus genetics. Similar association was found for estrous response at the end of the synchronization protocol, ranging from 66.3% to 38.4%, respectively. This reduced estrous response helped to explain the pregnancy results, once the pregnancy to AI of cows showing estrus was 2.3-fold greater than for those not showing estrus and submitted to TAI. Despite reduced pregnancy per AI, the increase in the proportion of B. indicus genetics of cows was not associated with a reduction in the proportion of pregnant cows at the end of the breeding season. Nevertheless, the interval from entering the breeding season to pregnancy was lengthened as the proportion of B. indicus genetics of cows increased. The median days to pregnancy was extended by 25 when the proportion of B. indicus genetics surpassed 78% compared with less than 20%. Thus, the increase in the proportion of B. indicus genetics of cows was related to a reduction in pregnancy per AI and lengthening the interval to attain pregnancy during the breeding season, but not with the final proportion of pregnant cows. As a result, reproductive management strategies directed specifically to cows with a greater proportion of B. indicus genetics are needed to improve the rate of pregnancy in beef herds.
Horizontal and vertical crossover of sine cosine algorithm with quick moves for optimization and feature selection
Authors: Hu Hanyu , Shan Weifeng , Tang Yixiang , Heidari Ali Asghar , Chen Huiling , Liu Haijun , Wang Maofa , Escorcia-Gutierrez José , Mansour Romany F , Chen Jun 
Published: November 2022

AbstractThe sine cosine algorithm (SCA) is a metaheuristic algorithm proposed in recent years that does not resort to nature-related metaphors but explores and exploits the search space with the help of two simple mathematical functions of sine and cosine. SCA has fewer parameters and a simple structure and is widely used in various fields. However, it tends to fall into local optimality because it does not have a well-balanced exploitation and exploration phase. Therefore, in this paper, a new, improved SCA algorithm (QCSCA) is proposed to improve the performance of the algorithm by introducing a quick move mechanism and a crisscross mechanism to SCA and adaptively improving one of the parameters. To verify the effectiveness of QCSCA, comparison experiments with some conventional metaheuristic algorithms, advanced metaheuristic algorithms, and SCA variants are conducted on IEEE CEC2017 and CEC2013. The experimental results show a significant improvement in the convergence speed and the ability to jump out of the local optimum of the QCSCA. The scalability of the algorithm is verified in the benchmark function. In addition, QCSCA is applied to 14 real-world datasets from the UCI machine learning database for selecting a subset of near-optimal features, and the experimental results show that QCSCA is still very competitive in feature selection (FS) compared to similar algorithms. Our experimental results and analysis show that QCSCA is an effective method for solving global optimization problems and FS problems.