bluuebunny/crossref_metadata_2025_split huggingface dataset:
Specific clinical characteristics are correlated with long-term SARS CoV-2 IgG titers
Authors: Schnapp Aviad , Gutbir Yuval , Ohana-Sarna Lea , Ben-Nachum Noa , Benenson Talya , Gilad Rivka , Lazaroff Heiley , Zamir Chen Stein , Shimon Reif , Wolf Dana , Kerem Eitan 
Published: August 2022

Abstract
Purpose
To investigate the correlation between various clinical characteristics and long-term SARS CoV-2 IgG following a COVID-19 outbreak in a school.
Methods
532 sera of polymerase chain reaction (PCR)-tested children, adolescents and adults were collected seven months following a large eruption of COVID-19 in a high school in Jerusalem. Anti-S IgG antibody titers were obtained, quantified and analyzed in relation to clinical symptoms resulting from SARS CoV-2 infection.
Results
Only 80% of PCR-positive individuals at the time of the outbreak were seropositive seven months following exposure. IgG positivity was well-correlated with PCR-positivity, as was the IgG titer (ß=0.56, p < 0.01). Other correlations established included young age (ß=-0.12, p < 0.01) and symptoms including fever (ß=0.11, p = 0.02) and weakness (ß=0.14, p = 0.01). Households of individuals with negative serologies had fewer symptomatic members than households of IgG-positive individuals (p < 0.01).
Conclusions
Clinical symptoms of fever and weakness may serve as predictive factors for long-term SARS-CoV-2 IgG following exposure to the virus.
Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway
Authors: Zhou Lv , Chen Xiaohao , Pu Junxia , Shi Junhao , Deng Yibin 
Published: August 2022

AbstractBackground Hepatocellular carcinoma (HCC) ranks the sixth most common cancer in the number of malignancies worldwide. Accumulating evidence indicated that circular RNAs (circRNAs) play vital roles in the progression of several malignancies. Nonetheless, the molecular mechanism and role of hsa_circ_0006421 in HCC are yet unclear. The present study aimed to verify the molecular mechanism and effects of hsa_circ_0006421 in HCC. Methods A total of 34 paired HCC tissues and paraneoplastic tissues surgically resected from patients were collected, and the expression of hsa_circ_0006421 in HCC tissues and the correlation with clinicopathological characteristics were analyzed. Then, CCK-8, colony formation, cell migration assay, transwell invasion assay, and Annexin-V/PI staining were used to assess the effects of hsa_circ_0006421 on the growth, migration, invasion, and apoptosis of HCC cells. Next, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to detect the expression of miR-134-5p, CELF2, and hsa_circ_0006421. Finally, the targeting interactions of miR-134-5p and hsa_circ_0006421, CELF2 and miR-134-5p were explored using the dual-luciferase reporter assay. Results Hsa_circ_0006421 was diminished in HCC tissues, and its downregulation was related to cirrhosis history. Hsa_circ_0006421 knockdown promoted HCC cell growth, migration, and invasion and inhibited apoptosis, whereas overexpression had opposite effects. Moreover, hsa_circ_0006421 served as the competing endogenous RNA of miR-134-5p. Subsequently, a reciprocal correlation between CELF2 and miR-134-5p was established. Hsa_circ_0006421 positively regulated the protein level of CELF2 in HCC. Conclusion Hsa_circ_0006421 inhibits liver cancer by regulating miR-134-5p/CELF2 axis.
Exploring medical students' perceptions of Family Medicine in Kyrgyzstan: A mixed method study
Authors: Ismailova Zhyldyz , Mambetalieva Damira , Brimkulov Nurlan , Beran David , Nendaz Mathieu , Vu Nu Viet , Loutan Louis , Baroffio Anne , Heller Olivia 
Published: August 2022

Abstract
Background Despite knowing that health systems with a strong primary care basis improve overall health outcomes within a population, many countries are facing a global trend of declining interest and shortage of family doctors. This is the case of the Kyrgyz Republic, in which rural areas are struggling to attract and retain family medicine (FM) doctors. This study aims to explore how Kyrgyz medical students perceive FM and the factors that influence their specialty choice.Methods This study used a cross-sectional explanatory sequential design, including quantitative survey and focus group discussions that were carried out at the Kyrgyz State Medical Academy (KSMA) in Bishkek in 2017. Overall, 66% (953 out of 1449) of medical undergraduate students registered in year 1, 4 and 6 completed the survey, and 42 participated in the focus groups. The results were organized around 7 factors influencing perceptions and attitudes towards FM identified through a qualitative systematic review by Olid et al. Results The interest of Kyrgyz students for FM was the lowest of all specialties. Access to high medical technologies, career opportunities, salary, patient interaction and possibility to work abroad were the five most important factors influencing specialty choice. FM was perceived as a difficult profession, yet with poor prestige, insufficient remuneration, limited career possibilities and poor working conditions, especially in rural areas. The academic discourse, which disregards FM specialty had a negative influence on student’s perceptions and prevented students' ability to identify with the practice of family medicine. However, students’ awareness of their social accountability arose as a positive leverage to increase the choice of FM, provided other problems were solved. Conclusion This study highlighted key factors responsible of the low number of students choosing to become FM in Kyrgyzstan. The first major factor, presumably specific to many LMICs was the poor working conditions in remote areas. The second factor, common to many countries, was the distorted image of FM and its specialty transmitted through the medical schools’ institutional culture which does not value FM through positive role models. This study served as a basis to establish a strategy to promote FM within the KSMA and potentially at National level.
How can we push social robot use cases in healthcare - An interoperable programming interface
Authors: Glauser Robin Paul , Holm Jürgen , Bender Matthias , Bürkle Thomas 
Published: August 2022

Abstract
Background: Currently, it is hard for non-robotics-experts to create healthcare use cases for robots. To improve this situation, we want to implement our own middleware. This middleware should hide the technical details from our students. With this it will be possible to create social robotic use cases without robotics domain knowledge. The new middleware should cover the existing requirements of being usable by non-experts. Existing robot middleware were researched, and we found that most are too complicated or outdated. This is the reason a new middleware was developed. We developed our new middleware based on Android. The middleware can be put over existing robot SDKs and middleware. It runs on the android tablet of the Cruzr robot. Some tooling for the middleware was developed as well, e.g. a web component to control the robot via a webinterface.Results: We created the new robot middleware and deployed it on a Cruzr robot. The middleware relies on the WebSocket API and includes a Python implementation. The middleware supports various robot functions e.g. text-to-speech, speech-to-text, navigation, displaying content and scanning bar codes. We can show that the middleware can run on a Pepper robot. Although we did not implement all functions.Conclusions: In a one week course the middleware held its ground. It was used by the students to implement healthcare use cases. The feedback about the middleware from the students was good as well. There were minor issues that we were able to correct or adjust for during the course. With the provided architecture, it is possible to port this interface to other robots. This allows us to then use all the tools and documentation created for this interface. As we made the interface easy to get started with, it is a good foundation for teaching non-technical staff. We showed that it is possible to run the middleware on the Pepper robot. For future student courses this means students can exchange code even if they didn't work with the same robot. We also share our look on cloud based and local speech services. We discuss what the middleware needs, to run without having to change any code on other robots. Also we give an outlook at how the programming interface can be made even simpler by using natural text to code generators. For other researchers using the mentioned platforms (Cruzr, Pepper) this new middleware can be used for testing human robot interaction. It could help implementing their own real life usecases and be used in a teaching setting as well. It is also possible to adapt the middleware to other robots using the same interface and philosophy of using simple methods.
Clinical utility of the neutrophil elastase inhibitor Sivelestat for the treatment of sepsis asscociated with both acute respiratory distress syndrome and Septic cardiomyopathy patients
Authors: Lv Hui , Shi Qingyuan , Huang Langjing , Xiao Shuna , Zhang Changdong , Shang Xiaoke 
Published: August 2022

Abstract
Background: Acute respiratory distress syndrome(ARDS) and Septic cardiomyopathy(SCM) are most serious complications of sepsis. We aimed to evaluate the effects of the neutrophil elastase inhibitor sivelestat for the treatment of sepsis induced ARDS and SCM. Methods: Seventy patients who were diagnosed with ARDS and SCM between January 2019 and December 2021 at our hospital were randomly divided into sivelestat-treated group and the control group. Serum concentrations of IL-6, IL-8, TNF-α and HMGB1 were compared at five time points(baseline, 12h, 24h, 48h and 72h after ICU admission). Cardiac function evaluation by color Doppler ultrasound and Heart rate variability evaluation by 24h Holter recording was assessed at the time of ICU admission and 72h after sivelestat treatment. Results:The levels of IL-6, IL-8, and TNF-αwere significantly lower in the sivelestat-treated group at different time points(12h, 24h, 48h and 72h). HMGB1 levels were significantly lower 72 h after ICU admission in the sivelestat-treated group(19.46±2.63pg/mL vs. 21.20±2.03pg/mL, P = 0.003). The SV, TAPSE, E/A, e’, and a’value were significantly low in the control group compared with the sivelestat-treated group. Tei index was high in the control group compared with the sivelestat-treated group (0.60±0.08 vs. 0.56±0.07, P = 0.029). The result of Heart rate variability showed there were significant differences in SDNN, LF and LF/HF between two groups. Conclusions: Sivelestat can significantly reduce the level of plasma inflammatory factors, improve cardiac function and reduce heart rate variability in patients with sepsis induced ARDS and SCM. The trial registration number: ChiCTR-OPC-17013149
Thi̇rdhand Smoke: Are Parents Aware of İt?
Authors: AKCA Gulfer , AKCA Unal 
Published: August 2022

Abstract
PURPOSEThis study aims to understand the beliefs about thirdhand smoke among parents or grandparents of children in Northern Turkey and to provide an evidence base to incorporate thirdhand smoke preventative action into tobacco control interventions.METHODSThis cross-sectional study were collected through face-to-face interviews in a tertiary training hospital. Total of 1016 parents make assessments on the 'beliefs about thirdhand smoke' (BATHS) scale. Sociodemographic details including age, gender, education level, smoking status, personal income, exposure in the home, car or outside, type of home ownership and awareness of the term third-hand smoke were investigated. Scale assessment, univariate and multivariate analyses to explore the factors influencing the BATHS scale and awareness of the term third-hand smoke, were performed using SPSS version 22.0.RESULTSParticipants who were grandparents,non smokers, higher incomes, those who have heard the term third-hand smoke and higher education levels were more likely to get higher scores on the BATHS scale (p<0.001). The level of awareness is 8.7%. Awareness of the term third-hand smoke was associated with education (OR=18.835;95% CI:5.205- 68.186, p<0.001), living own home (OR=2,667;95% CI:1.377-5.181, p=0.004). Smoking status, exposure in the home,car and ouside get higher BATHS score (p<0.001) but not significant with awereness (p>0.05).CONCLUSIONSThirdhand smoke harm belief was associated with education, enforced smoke-free home and car and attempts to quit smoking. Sensitizing parents to thirdhand smoke risk could guide targeted actions for smoke-free home interventions
Deciphering of seed Health of common food grains(wheat,rice) of North Eastern UP and Gurgaon Haryana, India
Authors: NK Narendra Kumar , Khurana SM Paul Khurana , VN Vashist. N. Pandey 
Published: August 2022

Abstract
The stored samples of food grains of wheat and rice(60 samples) were purchased from places of Eastern UP and Gurgaon district Haryana. Its moisture contents were estimated.The Mycological investigations of wheat revealed presence of a total number of 16 fungal species viz., Alternaria alternata, Aspergillus candidus ,Aspergillus flavus, A. niger, A. ochraceous ,A. phoenicis, A. tamari,A. terreus, A. sydowi ,Fusarium moniliforme, F. oxysporum F. solani ,P. glabrum ,Rhizopus nigricans, Trichoderma viride and Trichothecium roseum. While Mycological analysis of rice seeds showed presence of 15 fungal species viz., Alternaria padwickii, A. oryzae, Curvularia lunata, Fusarium moniliforme, Aspergillus clavatus, A. flavus, A. niger, Cladosporium sp., Nigrospora oryzae, Alternaria tenuissima, Chaetomium globosum, F. solani, Microascus cirrosus, Helminthosporium oryzae, Pyricularia grisea.It also projected variation in presence of fungal species in blotter and agar plate method of analysis. The insect analysis revealed that wheat samples were infected with Tribolium castaneum.While rice sample showed presence of insect Sitophilus oryzae. The investigations revealed that Aspergillus flavus, A.niger, Sitophilus oryzae and Tribolium castaneum caused reduction in seed weight loss, seed germination,carbohydrate and protein contents of common food grains(wheat,rice).It also revealed that randomly selected A. flavus isolate 1 of wheat showed higher potential of aflatoxin B1 production (1392.940 µg/l) while rice isolate 2 showed 1231.117 µg/l production.
Phenotypic, Molecular Detection And Antibiogram Analysis of Aeromonas Hydrophila from Oreochromis Niloticus (Nile Tilapia) And Ready-To- Eat Fish Products in selected Rift Valley Lakes of Ethiopia
Authors: Kerigano Nebiyu Kassa , Chibsa Tesfaye Rufael , Molla Yitbarek Getachew , Mohamed Abde Aliy , Tamiru Mekdes , Bulto Abebe Olani , Wodaj Tafesse Koran , Gebreweld Dereje Shegu , Abdi Alemu Kebede 
Published: August 2022

Abstract

Background: Aeromonas hydrophila is a zoonotic bacterial pathogen that frequently causes disease and mass mortalities among cultured and feral fishes worldwide. In Ethiopia, A. hydrophila outbreak was reported in Sebeta fish ponds and in Lake Tana fishery. However, there is no to little information on the molecular, and phenotypical characteristics of A. hydrophila in Ethiopian fisheries. Therefore, a cross-sectional study was conducted from November 2020 to May 2021 in selected Ethiopian Rift valley lakes.Results: A total of 140 samples were collected aseptically from fish (Muscle, Gill, Intestine, Spleen and Kidney) from fish landing sites, market and restaurants with purposive sampling methods. Aeromonas selective media (AMB), morphological and biochemical tests were used to isolate and identify A. hydrophila. Accordingly, the pathogen was isolated from 81 (60.45%) of samples. Among the isolates 92.59% expressed virulence trait through β hemolysis on blood agar media with 5% sheep blood. Moreover, 54 strains (66.67%) were further confirmed with Real-Time PCR (qPCR) using ahaI gene specific primers and optimized protocol. The highest (68.51%) were detected from live fish, (24.07%) were from market fish and the lowest (7.4%%) were from ready-to-eat products. Antibiogram analysis was conducted on ten representative isolates. Accordingly, A. hydrophila isolates were susceptible to ciprofloxacin (100%), chloramphenicol (100%) and ceftriaxone (100%). However, all ten isolates were resistant to Amoxicillin and Penicillin.Conclusions: The study indicates A. hydrophila strains carrying virulence ahaI gene that were ß-hemolytic and resistant to antibiotics commonly used in human and veterinary medicine are circulating in the fishery. The detection of the pathogen in 140 of the sampled fish population is alarming for potential outbreaks and zoonosis. Therefore, further molecular epidemiology of the disease should be studied to establish potential inter host transmission and antibiotic resistance traits. Therefore, raising the public awareness on risk associated with consuming undercooked or raw fish meat is pertinent.
What computations can be done with traveling waves in visual cortex?
Authors: Benigno Gabriel , Budzinski Roberto , Davis Zachary , Reynolds John , Muller Lyle 
Published: August 2022

Abstract
Recent analyses have found waves of neural activity traveling across entire visual cortical areas in awake animals. These traveling waves modulate excitability of local networks and perceptual sensitivity. The general computational role for these spatiotemporal patterns in the visual system, however, remains unclear. Here, we hypothesize that traveling waves endow the brain with the capacity to predict complex and naturalistic visual inputs. We present a new network model whose connections can be rapidly and efficiently trained to predict natural movies. After training, a few input frames from a movie trigger complex wave patterns that drive accurate predictions many frames into the future, solely from the network’s connections. When the recurrent connections that drive waves are randomly shuffled, both traveling waves and the ability to predict are eliminated. These results show traveling waves could play an essential computational role in the visual system by embedding continuous spatiotemporal structures over spatial maps.
A novel rectenna design: To make Wireless Sensor Network
Technology Affordable for Low-Income Countries
Authors: Sharma Priya , Singh Ashutosh , Naresh B , Singh Vinod 
Published: August 2022

Abstract
Wireless Sensor Networks have numerous applications in several fields like Military, HealthCare, Surveillance applications, Agriculture monitoring etc. It is worth mentioning here that developing countries and low income countries are still deprived of availing the benefits of wireless sensor networks (wsn) due to their high cost and poor life time. Since, most of the wireless sensor networks work in an unattended environment; therefore it is not possible to charge the motes. Motes become dead after getting discharged and huge expenditure in creating wireless sensor network becomes meaningless. We have no other option except to install some energy harvesting device in the motes to make the wsn technology economical and suitable for developing and low income countries. A novel and compact monopole conductor-backed coplanar waveguide (CBCPW) fed rectenna design is proposed for radio frequency (RF) energy harvesting in wireless sensor networks (WSN). The rectenna's maximum measured power conversion efficiency (PCE), tested at 2.5 GHz, 2KΩ of load resistance, and 1.89 V of output voltage, is determined to be 33.5%.
Efficacy of olive stones and corncobs crystalline silica nanoparticles (Sio2, NPs) treatments on potato tuber moths (Phthorimaea operculella)
Authors: Idris I. , Naddaf M. , Harmalani H. , Alshater R. , Alsafadi R. 
Published: August 2022

Abstract
Morphological and microstructural properties of silica (SiO2) are essential factors that need to be considered during the experimental applications. In this report, SiO2 nanoparticles (NPs) have been prepared at different concentrations from SiO2 powder derived from olive stones and corncobs by an alkali leaching extraction method. Thermal treatment has been used to modify the morphological and microstructural properties of the extracted SiO2. X-ray diffraction (XRD) shows that the extracted amorphous SiO2 has been transformed into a crystalline phase after the thermal treatment. Nitrogen adsorption-desorption measurements revealed significant reduction in both the specific surface area (SABET) and the total pores volume (Vtotal) of the derived SiO2 samples after the thermal treatment. The acquired thermal treatment properties of SiO2 found to have high impacts on the influence of the SiO2 NPs on the survival and development of larvae and pupae of P. opercullela. SiO2 NPs prepared from the crystalline silica samples exhibited less efficiency on suppressing potato tuber moth comparing to SiO2 NPs of the amorphous silica powders. Varied effects on biological parameters including larvae mortality, pupae weight, larval and pupal development time, fecundity and fertility are also linked to the SiO2 NPs source type and showed concentration depending manner effects. SiO2 NPs are conditionally effective as an alternative pesticide against P. opercullela, based on their sources, mesoporous structures and concentrations.
Genome-wide identification and expression analysis of the WRKY gene family in five legumes
Authors: Ling Lei , Zhao He , Chen Naiyu , Yin Yajie , Yue Hua , Qin Shumian , Zhao Xiaoju , Ren Guoling 
Published: August 2022

Abstract
Background: WRKY is a transcription factor with 60 highly conserved amino acid region, regulating physiological processes and development in many plants and respond to a variety of abiotic stresses. However, the WRKY gene family has not been systematically studied in multiple legumes.Results: In this study, genome-wide analysis of five legumes (Phaseolus vulgaris, Medicago truncatula, Lotus japonicus, Glycine max, Cicer arietinum) identified 90, 108, 83, 185 and 61 WRKY genes, respectively. Besides, the chromosomal localization, phylogenesis, conserved motifs, gene structure and gene replication analysis were proceed for WRKY genes. The results showed that the legumes WRKY gene branches from the same evolutionary system have similar protein structures. In addition, by analyzing the transcriptome data we figured out that the expression level of WRKY differed in each tissue. Meanwhile, qRT-PCR was carried out to investigate the expression patterns of WRKY under abiotic stress. The results show that WRKY protein play crucial regulatory roles under salt and drought stress. Conclusions: In summary, these results comprehensively describes the WRKY gene of five legumes, which provides a theoretical basis for further exploring the biological functions of members of the WRKY gene family in legumes.