bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knowledge, attitudes, and practices on food safety and hygiene of wet and dry fish handlers in Cox's Bazar, Bangladesh
Authors: Sobuj Mohammad Khairul Alam , Rabby Ahmad Fazley , Rahman Shafiqur , Hasan Shanur Jahedul , Bhowmik Shuva , Islam Md. Ariful , Islam Md. Mohidul , Mostofa Md. Golam , Mamun Abdullah‐Al 
Published: July 2022

AbstractThis study aimed to provide the very first description of the current scenario of knowledge, attitudes, and practices (KAP) concerning the food safety and hygiene subjects among wet fish handlers (WFHs) and dry fish handlers (DFHs) in Cox's Bazar, Bangladesh. Data collection was performed through the application of face‐to‐face interviews with 234 WFHs and 258 DFHs. The overall score of the correct answer assessed components was 55.95% and 57.05% in WFHs and DFHs, respectively. Among the different knowledge categories, both the respondents showed the highest positive response in the time, temperature, and quality control category and the lowest positive responses noted in the foodborne disease occurrence category. For attitudes, obtained results showed positive attitude with a mean score of 37.82 ± 4.28 and 35.58 ± 5.48 for WFHs and DFHs, respectively. The WFHs gained a mean score of 23.08 ± 4.24 for practices, and the score was 22.78 ± 4.47 in the case of DFHs. A positively significant correlation coefficient (rs) was observed among fish handlers' KAP of 0.326–0.584. Although the association between the three levels was acceptable, several food safety principles and hygiene practices remained unsatisfactory. These findings highlighted the need for rapid action to enhance food safety and hygiene KAP through an intensive training program to assure the production of safe fisheries products for human consumption.
Enzymatic Laser‐Induced Graphene Biosensor for Electrochemical Sensing of the Herbicide Glyphosate
Authors: Johnson Zachary T. , Jared Nathan , Peterson John K. , Li Jingzhe , Smith Emily A. , Walper Scott A. , Hooe Shelby L. , Breger Joyce C. , Medintz Igor L. , Gomes Carmen , Claussen Jonathan C. 
Published: July 2022

AbstractGlyphosate is a globally applied herbicide yet it has been relatively undetectable in‐field samples outside of gold‐standard techniques. Its presumed nontoxicity toward humans has been contested by the International Agency for Research on Cancer, while it has been detected in farmers’ urine, surface waters and crop residues. Rapid, on‐site detection of glyphosate is hindered by lack of field‐deployable and easy‐to‐use sensors that circumvent sample transportation to limited laboratories that possess the equipment needed for detection. Herein, the flavoenzyme, glycine oxidase, immobilized on platinum‐decorated laser‐induced graphene (LIG) is used for selective detection of glyphosate as it is a substrate for GlyOx. The LIG platform provides a scaffold for enzyme attachment while maintaining the electronic and surface properties of graphene. The sensor exhibits a linear range of 10–260 µm, detection limit of 3.03 µm, and sensitivity of 0.991 nA µm−1. The sensor shows minimal interference from the commonly used herbicides and insecticides: atrazine, 2,4‐dichlorophenoxyacetic acid, dicamba, parathion‐methyl, paraoxon‐methyl, malathion, chlorpyrifos, thiamethoxam, clothianidin, and imidacloprid. Sensor function is further tested in complex river water and crop residue fluids, which validate this platform as a scalable, direct‐write, and selective method of glyphosate detection for herbicide mapping and food analysis.
Spatiotemporal variation and sustainability of NDVI in the Yellow River basin
Authors: Dai Qin , Cui Chen‐feng , Wang Shuo 
Published: July 2022

AbstractAccurately determining vegetation coverage and its changes is very important to detect the effectiveness of the Grain for Green Project (GGP) in vegetation construction in the Yellow River basin. Moreover, an understanding of the impact of human activities and the future trends of vegetation cover allows for more effective maintenance of the achievements of ecological construction. Based on the normalized difference vegetation index (NDVI) of SPOT/VEGETATION satellite remote sensing data from 2000 to 2017, the spatiotemporal patterns of the vegetative cover in the Yellow River basin in the last 18 years were studied using linear regression, residual analysis, and the Hurst index. The vegetation coverage of the Yellow River basin was higher in the southeast and lower in the northwest, with a relatively slow growth trend. The whole basin NDVI was relatively stable, and only approximately one‐fifth of the region had a large fluctuation, mainly distributed in Ningxia and northern Shaanxi. The residual trend of the NDVI was positive in 87% of the areas in the basin, and the overall impact of human activities on the increase in NDVI was mainly promoted. The characteristics of vegetation change in the future with the same directionality were stronger than the reverse characteristics, but the area showing continuous degradation in the future still accounted for 7%, mainly distributed in Xining City, Lanzhou City, Ningxia Plain, Hetao Plain, etc., which needs further strengthening of ecological construction and protection. This study is helpful for predicting and evaluating the vegetation dynamics of the Yellow River basin under the background of GGP implementation and provides a theoretical basis for regional environmental protection and the future adjustment of GGP work.
The challenges of cloud adoption among South African small to medium enterprises: A thematic analysis
Authors: Mudzamba Ronald , van der Schyff Karl , Renaud Karen 
Published: July 2022

AbstractThe successful use of Information and Communication Technologies (ICTs) by Small to Medium Enterprises (SMEs) remains a persistent problem within developing economies where they face several technical and skills‐related challenges. Although cloud services can mitigate some of these challenges, many SMEs fail to adopt cloud services. Therefore, the main objective of this paper was to develop key recommendations (based on a high‐level cloud services adoption framework) which South African SMEs could use to guide them in their adoption of cloud services. To develop our recommendations, we conducted 13 semi‐structured interviews with nine South African SMEs within the Eastern Cape province. These were thematically analyzed and the findings used to create a high‐level cloud services adoption framework. Our findings indicate that technical expertise (i.e., knowledge) amongst SMEs plays a vital role in the cloud adoption process. Those SMEs who do realize the importance of such technical expertise often use intermediaries to achieve successful adoption of cloud services. We also found evidence to suggest that SMEs generally do not carry out comprehensive cloud adoption preparatory activities, consequently failing in their adoption of cloud services.
Visible red light does not induce DNA damage in human dermal fibroblasts
Authors: Wang Jennifer Y. , Austin Evan , Jagdeo Jared 
Published: July 2022

AbstractVisible red light (RL) therapy is a rapidly expanding treatment option for dermatological conditions, including acne, psoriasis and chronic wounds. It is currently unknown if high fluences of RL induce DNA damage via reactive oxygen species (ROS) stress or other pathways. Our lab previously demonstrated that RL generates ROS in human dermal fibroblasts (HDFs). Other studies show that UV and blue light generate ROS and DNA damage in fibroblasts. This study aims to determine if RL induces DNA damage in HDFs. We found that 320 J/cm2, 640 J/cm2 and 1280 J/cm2 RL (633 ± 6 nm) did not measurably increase DNA damage in the form of cyclobutane pyrimidine dimers (CPD) or 6‐4 photoproducts (6‐4PP) immediately, 3 hours and 24 hours following irradiation. Our study further supports that RL therapy is safe in human skin fibroblasts.
The proteomic and particle composition of human platelet lysate for cell therapy products
Authors: Rodrigues Raul M. , Valim Vanessa de Souza , Berger Markus , da Silva Annelise P. M. , Fachel Flávia N. S. , Wilke Ianaê I. , da Silva Walter O. B. , Santi Lucélia , da Silva Maria A. L. , Amorin Bruna , Sehn Filipe , Yates John R. , Guimarães Jorge A. , Silla Lucia 
Published: July 2022

AbstractFollowing health agencies warning, the use of animal origin supplements should be avoided in biological products proposed as therapy in humans. Platelet lysate and several other growth factors sources are alternatives to replace fetal calf serum, the current gold standard in clinical‐grade cell culture. However, the platelet supplement's content lacks data due to different production methods. The principle behind these products relays on the lysis of platelets that release several proteins, some of which are contained in heterogeneous granules and coordinate biological functions. This study aims to analyze the composition and reproducibility of a platelet lysate produced with a standardized method, by describing several batches' protein and particle content using proteomics and dynamic light scattering. Proteomics data revealed a diversified protein content, with some related to essential cellular processes such as proliferation, morphogenesis, differentiation, biosynthesis, adhesion, and metabolism. It also detected proteins responsible for activation and binding of transforming growth factor beta, hepatocyte growth factor, and insulin‐like growth factor. Total protein, biochemical, and growth factors quantitative data showed consistent and reproducible values across batches. Novel data on two major particle populations is presented, with high dispersion level at 231 ± 96 d.nm and at 30 ± 8 d.nm, possibly being an important way of protein trafficking through the cellular microenvironment. This experimental and descriptive analysis aims to support the content definition and quality criteria of a cell supplement for clinical applications.
Vasorin plays a critical role in vascular smooth muscle cells and arterial functions
Authors: Louvet Loïc , Lenglet Gaëlle , Krautzberger A. Michaela , Mentaverri Romuald , Hague Frédéric , Kowalewski Clara , Mahtal Nassim , Lesieur Julie , Bonnet Anne‐Laure , Andrique Caroline , Gaucher Céline , Gomila Cathy , Schrewe Heinrich , Tharaux Pierre‐Louis , Kamel Said , Chaussain Catherine , Six Isabelle 
Published: July 2022

AbstractWithin the cardiovascular system, the protein vasorin (Vasn) is predominantly expressed by vascular smooth muscle cells (VSMCs) in the coronary arteries and the aorta. Vasn knockout (Vasn−/−) mice die within 3 weeks of birth. In the present study, we investigated the role of vascular Vasn expression on vascular function. We used inducible Vasn knockout mice (VasnCRE‐ERT KO and VasnSMMHC‐CRE‐ERT2 KO, in which respectively all cells or SMCs only are targeted) to analyze the consequences of total or selective Vasn loss on vascular function. Furthermore, in vivo effects were investigated in vitro using human VSMCs. The death of VasnCRE‐ERT KO mice 21 days after tamoxifen injection was concomitant with decreases in blood pressure, angiotensin II levels, and vessel contractibility to phenylephrine. The VasnSMMHC‐CRE‐ERT2 KO mice displayed concomitant changes in vessel contractibility in response to phenylephrine and angiotensin II levels. In vitro, VASN deficiency was associated with a shift toward the SMC contractile phenotype, an increase in basal intracellular Ca2+ levels, and a decrease in the SMCs' ability to generate a calcium signal in response to carbachol or phenylephrine. Additionally, impaired endothelium‐dependent relaxation (due to changes in nitric oxide signaling) was observed in all Vasn knockout mice models. Our present findings highlight the role played by Vasn SMC expression in the maintenance of vascular functions. The mechanistic experiments suggested that these effects are mediated by SMC phenotype switching and changes in intracellular calcium homeostasis, angiotensin II levels, and NO signaling.
A critical role of AMP‐activated protein kinase in regulating intestinal nutrient absorption, barrier function, and intestinal diseases
Authors: Wu Zhihui , Xu Chengfei , Zheng Tenghui , Li Qihui , Yang Siwang , Shao Jiayuan , Guan Wutai , Zhang Shihai 
Published: July 2022

AbstractAs one of the most important organs in animals, the intestine is responsible for nutrient absorption and acts as a barrier between the body and the environment. Intestinal physiology and function require the participation of energy. 5′‐adenosine monophosphate‐activated protein kinase (AMPK), a classical and highly expressed energy regulator in intestinal cells, regulates the process of nutrient absorption and barrier function and is also involved in the therapy of intestinal diseases. Studies have yielded findings that AMPK regulates the absorption of glucose, amino acids, and fatty acids in the intestine primarily by regulating transportation systems, as we detailed here. Moreover, AMPK is involved in the regulation of the intestinal mechanical barrier and immune barrier through manipulating the expression of tight junctions, antimicrobial peptides, and secretory immunoglobulins. In addition, AMPK also participates in the regulation of intestinal diseases, which indicates that AMPK is a promising therapeutic target for intestinal diseases and cancer. In this review, we summarized the current understanding regarding how AMPK regulates intestinal nutrient absorption, barrier function, and intestinal diseases.
Association of histo‐blood group antigens and predisposition to gastrointestinal diseases
Authors: Saikia Kasturi , Saharia Niruprabha , Singh Chongtham S. , Borah Partha P. , Namsa Nima D. 
Published: July 2022

AbstractInfectious gastroenteritis is a common illness afflicting people worldwide. The two most common etiological agents of viral gastroenteritis, rotavirus and norovirus are known to recognize histo‐blood group antigens (HBGAs) as attachment receptors. ABO, Lewis, and secretor HBGAs are distributed abundantly on mucosal epithelia, red blood cell membranes, and also secreted in biological fluids, such as saliva, intestinal content, milk, and blood. HBGAs are fucosylated glycans that have been implicated in the attachment of some enteric pathogens such as bacteria, parasites, and viruses. Single nucleotide polymorphisms in the genes encoding ABO (H), fucosyltransferase gene FUT2 (Secretor/Se), FUT3 (Lewis/Le) have been associated with changes in enzyme expression and HBGAs production. The highly polymorphic HBGAs among different populations and races influence genotype‐specific susceptibility or resistance to enteric pathogens and its epidemiology, and vaccination seroconversion. Therefore, there is an urgent need to conduct population‐based investigations to understand predisposition to enteric infections and gastrointestinal diseases. This review focuses on the relationship between HBGAs and predisposition to common human gastrointestinal illnesses caused by viral, bacterial, and parasitic agents.
A new intelligent approach for size optimization of a renewable energy based grid connected hybrid energy system
Authors: Sharma Bandana , Rizwan Mohammad , Anand Priyanka 
Published: July 2022

AbstractAdopting hybrid power systems to provide access to clean, reliable, and inexpensive energy is necessary for countries like India to accomplish their goals of sustainable development. This work proposes an optimization approach for sizing a grid‐connected hybrid renewable energy system (HRES) that includes photovoltaic, biomass, biogas, and a battery. In this research, a recently developed Aquila optimization algorithm is utilized to evaluate the optimal size of HRES for a cluster of villages near Sarai Jairam, Agra district, Uttar Pradesh, India using the MATLAB software package. The goal of this optimization approach is to reduce the net present cost for HRES while satisfying the operational constraints, including the reliability of the power supply and maximum utilization of the photovoltaic, biomass, and biogas complementary properties. Initially, three HRES configurations in the off‐grid mode were optimized using the suggested Aquila optimization technique. To confirm the findings, the optimization results for the same models of off‐grid HRES were obtained using the harmony search (HS) and particle swarm optimization (PSO) algorithms. The same algorithms were also used to optimize the grid‐connected SPV/biomass/biogas with the battery model of HRES. Finally, the outcomes from the off‐grid models presented above were compared to the grid‐linked model. The grid‐connected configuration which is obtained by the Aquila optimization approach provides an optimal solution with the least net present cos t (NPC) and minimum cost of energy (COE) when compared with HS and PSO. The optimal grid‐connected HRES includes 235 kW of PV, a 10 kW biogas generator, a 64 kW biomass generator, and a 50.40 kWh battery bank. The overall net present cost and the COE are found to be $547 670 and $0.0768/kWh, respectively.
Abaloparatide outperforms teriparatide in protecting against alveolar bone loss in experimental periodontitis
Authors: Li Yuan , Qian Yuran , Qiao Hui , Pan Weiyi , Xie Liang , Li Yu 
Published: July 2022

AbstractBackgroundThe aim of this study was to compare the effects of two osteoanabolic drugs, abaloparatide (ABL) and teriparatide (TPTD), on protecting alveolar bone in experimental periodontitis.MethodsTwenty‐four 9‐week‐old, male, Sprague‐Dawley rats were placed with silk suture around the right maxillary second molar, and then were randomly divided into three groups, that is, the ABL, TPTD, and saline group, receiving intermittent subcutaneous injections of ABL (80 μg/kg), TPTD (80 μg/kg) or saline respectively every other day for 4 weeks. Samples on both sides were assessed through micro‐computerized tomography, histological, and immunohistochemical analysis. Mouse pre‐osteoblast MC3T3 cell was cultured with lipopolysaccharide (LPS) and treated with ABL or TPTD, before assays of cell proliferation, alkaline phosphatase (ALP) activity and real‐time polymerase chain reaction.ResultsOn the ligature side, both ABL and TPTD significantly reduced alveolar bone loss, and ABL had significantly better effects with higher expression of runt‐related transcription factor 2 (RUNX2) and Bglap (formerly called osteocalcin); meanwhile, the ligature induced osteoclastogenesis and down‐regulation of osteoprotegerin (OPG) was affected by neither drug. On the non‐ligature side, ABL also showed better osteoanabolic effects. In vitro studies revealed that, in the presence of LPS, ABL, and TPTD similarly promoted MC3T3 proliferation, whereas ABL induced higher ALP activity and osteoblastic gene expression compared to TPTD.ConclusionBoth ABL and TPTD protect and regenerate alveolar bone in experimental periodontitis, and ABL behaves even better than TPTD at the same dose, attributed to its stronger osteoanabolic effects in this context.
Acceleration effect of galacturonic acid on acrylamide generation: evidence in model reaction systems
Authors: Wang Pengpu , Sun Guoyu , Lu Pei , Zhu Yuchen , Hu Xiaosong , Chen Fang 
Published: July 2022

AbstractBACKGROUNDAcrylamide (AA) is a potential carcinogen formed in food rich in carbohydrate during heating. Recently, AA has been found in several fruit products, such as prune juice, sugarcane molasses and canned black olives. This study focused on the role of galacturonic acid (GalA), the main acid hydrolysis product of fruit pectin, in AA formation in three model systems – asparagine (Asn)/glucose (Glc), Asn/GalA, and Asn/Glc/GalA – during heating under different pH values (pH 3.8–7.8), Glc concentration (0–0.1 mol L−1), molar ratio of substrates (Asn/Glc = 1:1, 0.025–0.5 mol L−1) and temperature (120–180 °C) for 30 min, respectively.RESULTSThe results suggested that the addition of 0.1 mol L−1 GalA strongly accelerated AA formation in a manner dependent on pH value and temperature (P < 0.05). AA concentration under different Glc concentration and molar ratio of substrates suggested that GalA was more reactive than Glc when reacted with Asn. Furthermore, the Amadori rearrangement product/Schiff base/oxazolidine‐5‐one were identified as the intermediates formed in the Asn/GalA model system using ultra‐performance liquid chromatography–quadrupole‐time‐of‐flight–mass spectrometry.CONCLUSIONThe results suggested that Maillard reaction between Asn and GalA might contribute to AA formation. This study is significant in elucidating the contribution of interaction between components for AA formation in fruit products. © 2022 Society of Chemical Industry.
Development of stability indicating reversed‐phase high‐performance liquid chromatography method for determination of elobixibat in pure form and laboratory prepared tablets: Application to dissolution study
Authors: Elagamy Samar H. , Mansour Fotouh R. , Elbastawissy Almoataz Bellah B. , EL‐Malla Samah F. 
Published: July 2022

A simple stability‐indicating reversed‐phase high‐performance liquid chromatography method has been developed for determination of elobixibat in presence of its potential impurities and degradation products. The chromatographic separation was carried on a Thermo scientific Base Deactivated Silica BDS Hypersil‐C18 (150 × 4.6 mm; 5 μm) column using a mobile phase of acetonitrile and phosphate buffer (25 mM, pH 2.5) in a ratio of (70:30, v/v). The experimental conditions were accurately investigated, and the method was validated according to ICH guidelines Q2 (R1). The drug was subjected to various stress conditions including acidic, basic, oxidative, and photolytic conditions. The method successfully separates the drug from the three reported impurities and different degradants. The method was also successfully applied for the determination of elobixibat in laboratory prepared tablets (5.0 mg). Analysis shows no interference from excipients and degradation products. The method was also applied for performing in vitro dissolution testing of elobixibat laboratory prepared tablets. Since elobixibat is recently introduced into the market, there are no previous stability studies and no reported analytical methods for its determination. Thus, this study presents a validated and selective method that can be effectively employed in routine quality control studies.
On the determination of partial‐lives of dissipation and/or sorption of a solute in liquid‐solid systems
Authors: Shahwan Talal 
Published: July 2022

AbstractIn most sorption systems, chemical equilibrium is reached before achieving complete removal of the sorbate/solute from solution. In such cases, the determination of the partial‐life values of sorbate removal using the classical approach might lead to erroneous results, since that approach assumes ultimately total dissipation of the sorbate/solute. In order to overcome this limitation, a new approach is used in this work to develop novel expressions of the partial‐life of dissipation and the partial‐life of sorption of a single sorbate (or solute) in liquid‐solid batch systems. The expressions are formulated using both first and second order rate equations, in a manner which takes into account the equilibrium concentration of the sorbate/solute. For the sake of preliminary evaluation, the expressions are tested on previously published data for various systems. It is suggested that the proposed method for the determination of partial lives is useful in improving the reliability of the analysis of kinetic data of sorption systems, in particular those which involve sorbents of high surface activity and fast kinetics, like nanomaterials.