bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plate Boundary Deformation and Volcano Unrest at the Azores Triple Junction Determined From Continuous GPS Measurements, 2002–2017
Authors: D’Araújo J. , Sigmundsson F. , Ferreira T. , Okada J. , Lorenzo M. , Silva R. 
Published: January 2022

AbstractGround deformation in the Azores, at the triple junction between the Eurasian, Nubian, and North American plates, has been mapped with continuous Global Positioning System (GPS) geodetic measurements to improve tectonic motion estimates and for understanding volcanic unrest. We compute daily positions, spanning almost 17 years (2000–2017), from 18 continuous GPS stations. The GPS time‐series are analyzed by searching for discontinuities and periodic functions. Results show that Flores and Graciosa islands have displacements close to predicted North American and Eurasian plate motions, respectively, while São Miguel, Terceira, São Jorge, Faial and Pico islands have displacements in between predicted Eurasian and Nubian plate motions. The Eurasian‐Nubian plate boundary in the Azores behaves as a diffuse ultra‐slow oblique spreading center with focused deformation found in the central group (CG) and São Miguel islands. The velocity field is modeled by approximating segments of the Eurasian‐Nubian plate boundary with vertical dislocations with right‐lateral motion and opening below a locking depth. Best fitting models have deep motion in the range of 2.0–2.3 mm yr−1 directed N (82.3–88.7)°E. Such displacement accounts for almost half of predicted Eurasian‐Nubian relative plate motion. The modeling results suggest that the locking depth in the CG islands is at least 20 km while in São Miguel it is about 2 km. We find transient deformation at Fogo volcano, São Miguel Island, due to unrest activity mainly during 2003–2006 and 2011–2012, and local continuous subsidence in Terceira Island, attributed to a deflation source centered on the island.
The Onset of Gondwanide Orogeny in Eastern Australia: Insight From the Provenance of Syn‐Orogenic Strata in the New England Orogen (Australia)
Authors: Campbell Matthew J. , Hoy Derek , Rosenbaum Gideon , Fielding Christopher , Allen Charlotte M. 
Published: January 2022

AbstractThe last major episode of cordilleran‐style tectonism in eastern Australia was the late Paleozoic‐early Mesozoic Gondwanide Orogeny. When exactly this deformation commenced and what caused this phase of orogenesis is still debated. Using previous stratigraphic and sedimentological data from pre‐orogenic to syn‐orogenic strata, integrated with new detrital zircon U‐Pb geochronology, we investigated the onset of the Gondwanide Orogeny in the northern New England Orogen (Gogango Overfolded Zone, eastern Australia). The lowermost syn‐orogenic strata display evidence of rapid base‐level change coeval with increasingly abundant and more proximal mass‐wasting deposits, which reflect a shift to static loading‐induced subsidence and the establishment of a ∼1,600‐km long foreland basin system. The syn‐orogenic strata contain abundant detritus sourced from syn‐depositional magmatism, alongside an up‐section increase in detritus derived from uplifted older rocks of the New England Orogen. Detrital zircons show maximum depositional age of ∼276 Ma from immediately above the sequence boundary, and ∼269 Ma from an overlying formation. These results confirm previous suggestions that tectonic forcing of the orogenic hinterland affected subsidence and sedimentation patterns in the easternmost part of the basin during the middle Permian, >11 Myr prior to deformation in the western part of the foreland basin. The onset of the Gondwanide Orogeny in eastern Australia, and in other sectors of the Gondwanan margin, likely resulted from a tectonic switch from crustal extension to contraction, which was driven by increased convergence rates due to a plate reorganization event following the final assembly of Pangea.
Inhibition of Trpv4 rescues circuit and social deficits unmasked by acute inflammatory response in a Shank3 mouse model of Autism
Authors: Tzanoulinou Stamatina , Musardo Stefano , Contestabile Alessandro , Bariselli Sebastiano , Casarotto Giulia , Magrinelli Elia , Jiang Yong-hui , Jabaudon Denis , Bellone Camilla 
Published: January 2022

AbstractMutations in theSHANK3gene have been recognized as a genetic risk factor for Autism Spectrum Disorder (ASD), a neurodevelopmental disease characterized by social deficits and repetitive behaviors. While heterozygousSHANK3mutations are usually the types of mutations associated with idiopathic autism in patients, heterozygous deletion ofShank3gene in mice does not commonly induce ASD-related behavioral deficit. Here, we used in-vivo and ex-vivo approaches to demonstrate that region-specific neonatal downregulation ofShank3in the Nucleus Accumbens promotes D1R-medium spiny neurons (D1R-MSNs) hyperexcitability and upregulates Transient Receptor Potential Vanilloid 4 (Trpv4)to impair social behavior. Interestingly, genetically vulnerableShank3+/−mice, when challenged with Lipopolysaccharide to induce an acute inflammatory response, showed similar circuit and behavioral alterations that were rescued by acute Trpv4 inhibition. Altogether our data demonstrate shared molecular and circuit mechanisms between ASD-relevant genetic alterations and environmental insults, which ultimately lead to sociability dysfunctions.
Plant neighborhood shapes diversity and reduces interspecific variation of the phyllosphere microbiome
Authors: Meyer Kyle M , Porch Robert , Muscettola Isabella E , Vasconcelos Ana Luisa S , Sherman Julia K , Metcalf C Jessica E , Lindow Steven E , Koskella Britt 
Published: January 2022

Abstract
Microbial communities associated with plant leaf surfaces (i.e., the phyllosphere) are increasingly recognized for their role in plant health. While accumulating evidence suggests a role for host filtering of its microbiota, far less is known about how community composition is shaped by dispersal, including from neighboring plants. We experimentally manipulated the local plant neighborhood within which tomato, pepper, or bean plants were grown in a 3-month field trial. Focal plants were grown in the presence of con- or hetero-specific neighbors (or no neighbors) in a fully factorial combination. At 30-day intervals, focal plants were harvested and replaced with a new age- and species-matched cohort while allowing neighborhood plants to continue growing. Bacterial community profiling revealed that the strength of host filtering effects (i.e., interspecific differences in composition) decreased over time. In contrast, the strength of neighborhood effects increased over time, suggesting dispersal from neighboring plants becomes more important as neighboring plant biomass increases. We next implemented a cross-inoculation study in the greenhouse using inoculum generated from the field plants to directly test host filtering of microbiomes while controlling for directionality and source of dispersal. This experiment further demonstrated that focal host species, the host from which the microbiome came, and in one case the donor hosts’ neighbors, contribute to variation in phyllosphere bacterial composition. Overall, our results suggest that local dispersal is a key factor in phyllosphere assembly, and that demographic factors such as nearby neighbor identity and biomass or age are important determinants of phyllosphere microbiome diversity.
Risk for opioid misuse in chronic pain patients is associated with endogenous opioid system dysregulation
Authors: Ballester Javier , Baker Anne K. , Martikainen Ilkka K. , Koppelmans Vincent , Zubieta Jon-Kar , Love Tiffany M. 
Published: January 2022

Abstractµ-Opioid receptors (MOR) are a major target of endogenous and exogenous opioids, including opioid pain medications. The µ-opioid neurotransmitter system is heavily implicated in the pathophysiology of chronic pain and opioid use disorder and, as such, central measures of µ-opioid system functioning are increasingly being considered as putative biomarkers for risk to misuse opioids. To explore the relationship between MOR system function and risk for opioid misuse, 28 subjects with chronic nonspecific back pain completed a clinically validated measure of opioid misuse risk, the Pain Medication Questionnaire (PMQ), and were subsequently separated into high (PMQ > 21) and low (PMQ ≤ 21) opioid misuse risk groups. Chronic pain patients along with 15 control participants underwent two separate [11C]-carfentanil positron emission tomography scans to explore MOR functional measures: one at baseline and one during a sustained pain-stress challenge, with the difference between the two providing an indirect measure of stress-induced endogenous opioid release. We found that chronic pain participants at high risk for opioid misuse displayed higher baseline MOR availability within the right amygdala relative to those at low risk. By contrast, patients at low risk for opioid misuse showed less pain-induced activation of MOR-mediated, endogenous opioid neurotransmission in the nucleus accumbens. This study links human in vivo MOR system functional measures to the development of addictive disorders and provides novel evidence that MORs and µ-opioid system responsivity may underlie risk to misuse opioids among chronic pain patients.
Imbalance between default mode and sensorimotor connectivity is associated with perseverative thinking in obsessive-compulsive disorder
Authors: Stern Emily R. , Eng Goi Khia , De Nadai Alessandro S. , Iosifescu Dan V. , Tobe Russell H. , Collins Katherine A. 
Published: January 2022

AbstractObsessive-compulsive disorder (OCD) is highly heterogeneous. Although perseverative negative thinking (PT) is a feature of OCD, little is known about its neural mechanisms or relationship to clinical heterogeneity in the disorder. In a sample of 85 OCD patients, we investigated the relationships between self-reported PT, clinical symptom subtypes, and resting-state functional connectivity measures of local and global connectivity. Results indicated that PT scores were highly variable within the OCD sample, with greater PT relating to higher severity of the “unacceptable thoughts” symptom dimension. PT was positively related to local connectivity in subgenual anterior cingulate cortex (ACC), pregenual ACC, and the temporal poles—areas that are part of, or closely linked to, the default mode network (DMN)—and negatively related to local connectivity in sensorimotor cortex. While the majority of patients showed higher local connectivity strengths in sensorimotor compared to DMN regions, OCD patients with higher PT scores had less of an imbalance between sensorimotor and DMN connectivity than those with lower PT scores, with healthy controls exhibiting an intermediate pattern. Clinically, this imbalance was related to both the “unacceptable thoughts” and “symmetry/not-just-right-experiences” symptom dimensions, but in opposite directions. These effects remained significant after accounting for variance related to psychiatric comorbidity and medication use in the OCD sample, and no significant relationships were found between PT and global connectivity. These data indicate that PT is related to symptom and neural variability in OCD. Future work may wish to target this circuity when developing personalized interventions for patients with these symptoms.
Somatic copy number alteration predicts clinical benefit of lung adenocarcinoma patients treated with cytokine-induced killer plus chemotherapy
Authors: Kou Fan , Wu Lei , Zhu Ye , Li Baihui , Huang Ziqi , Ren Xiubao , Yang Lili 
Published: January 2022

AbstractSomatic copy number alterations (SCNA), which are widespread in cancer, can predict the efficacy of immune checkpoint inhibitors in non-small-cell lung cancer (NSCLC). However, the usefulness of SCNA for predicting the survival of patients treated with cytokine-induced killer (CIK) cells or chemotherapy (CT) is unknown. This study aimed to explore the correlation between SCNA and clinical outcome in NSCLC patients treated with CIK + CT or CT alone. We performed whole-exome sequencing on 45 NSCLC patients treated with CIK + CT, as well as 305 NSCLC patients treated with CT alone, from The Cancer Genome Atlas, which showed SCNA had a superiority in predicting the progression-free survival (PFS) over tumor mutation burden (TMB) and SCNA + TMB in NSCLC patients treated with CIK + CT, especially in lung adenocarcinoma, while SCNA could not predict the efficacy of CT alone. Additionally, we investigated the association between SCNA and immune cell infiltration by RNA sequencing and immunohistochemistry. The results revealed that SCNA was negatively associated with the expression of dendritic cells. Collectively, this study revealed a negative correlation between SCNA and response to CIK + CT and showed that SCNA is a predictive indicator in LUAD patients treated with CIK + CT.
SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth
Authors: Yu Yu-qiang , Thonn Veronika , Patankar Jay V. , Thoma Oana-Maria , Waldner Maximilian , Zielinska Marta , Bao Li-li , Gonzalez-Acera Miguel , Wallmüller Stefan , Engel Felix B. , Stürzl Michael , Neurath Markus F. , Liebing Eva , Becker Christoph 
Published: January 2022

AbstractSMYD2 is a histone methyltransferase, which methylates both histone H3K4 as well as a number of non-histone proteins. Dysregulation of SMYD2 has been associated with several diseases including cancer. In the present study, we investigated whether and how SMYD2 might contribute to colorectal cancer. Increased expression levels of SMYD2 were detected in human and murine colon tumor tissues compared to tumor-free tissues. SMYD2 deficiency in colonic tumor cells strongly decreased tumor growth in two independent experimental cancer models. On a molecular level, SMYD2 deficiency sensitized colonic tumor cells to TNF-induced apoptosis and necroptosis without affecting cell proliferation. Moreover, we found that SMYD2 targeted RIPK1 and inhibited the phosphorylation of RIPK1. Finally, in a translational approach, pharmacological inhibition of SMYD2 attenuated colonic tumor growth. Collectively, our data show that SMYD2 is crucial for colon tumor growth and inhibits TNF-induced apoptosis and necroptosis.
Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats
Authors: Gong Juexiao , Luo Man , Yong Yonghong , Zhong Shan , Li Peng 
Published: January 2022

AbstractAlamandine (Ala) is a novel member of the renin–angiotensin-system (RAS) family. The present study aimed to explore the effects of Ala on hypertension and renal damage of Dahl salt-sensitive (SS) rats high-salt diet-induced, and the mechanisms of Ala on renal-damage alleviation. Dahl rats were fed with high-salt diets to induce hypertension and renal damage in vivo, and HK-2 cells were treated with sodium chloride (NaCl) to induce renal injury in vitro. Ala administration alleviated the high-salt diet-induced hypertension, renal dysfunction, and renal fibrosis and apoptosis in Dahl SS rats. The HK-2 cells’ damage, and the increases in the levels of cleaved (c)-caspase3, c-caspase8, and c-poly(ADP-ribose) polymerase (PARP) induced by NaCl were inhibited by Ala. Ala attenuated the NaCl-induced oxidative stress in the kidney and HK-2 cells. DETC, an inhibitor of SOD, reversed the inhibitory effect of Ala on the apoptosis of HK-2 cells induced by NaCl. The NaCl-induced increase in the PKC level was suppressed by Ala in HK-2 cells. Notably, PKC overexpression reversed the moderating effects of Ala on the NaCl-induced apoptosis of HK-2 cells. These results show that Ala alleviates high-salt diet-induced hypertension and renal dysfunction. Ala attenuates the renal damage via inhibiting the PKC/reactive oxygen species (ROS) signaling pathway, thereby suppressing the apoptosis in renal tubular cells.
Feasibility and clinical utility of handheld fundus cameras for retinal imaging
Authors: Das Susmit , Kuht Helen J. , De Silva Ian , Deol Sundeep S. , Osman Lina , Burns Joyce , Sarvananthan Nagini , Sarodia Usman , Kapoor Bharat , Islam Tahir , Sampath Raghavan , Poyser Alicia , Konidaris Vasileios , Anzidei Rossella , Proudlock Frank A. , Thomas Mervyn G. 
Published: January 2022

Abstract
Background/objectives
Handheld fundus cameras are portable and cheaper alternatives to table-top counterparts. To date there have been no studies comparing feasibility and clinical utility of handheld fundus cameras to table-top devices. We compare the feasibility and clinical utility of four handheld fundus cameras/retinal imaging devices (Remidio NMFOP, Volk Pictor Plus, Volk iNview, oDocs visoScope) to a table-top camera (Zeiss VisucamNM/FA).

Subjects/methods
Healthy participants (n = 10, mean age ± SD = 21.0 ± 0.9 years) underwent fundus photography with five devices to assess success/failure rates of image acquisition. Participants with optic disc abnormalities (n = 8, mean age ± SD = 26.8 ± 15.9) and macular abnormalities (n = 10, mean age ± SD = 71.6 ± 15.4) underwent imaging with the top three scoring fundus cameras. Images were randomised and subsequently validated by ophthalmologists masked to the diagnoses and devices used.

Results
Image acquisition success rates (100%) were achieved in non-mydriatic and mydriatic settings for Zeiss, Remidio and Pictor, compared with lower success rates for iNview and oDocs. Image quality and gradeability were significantly higher for Zeiss, Remidio and Pictor (p < 0.0001) compared to iNview and oDocs. For cup:disc ratio estimates, similar levels of bias were seen for Zeiss (−0.09 ± SD:0.15), Remidio (−0.07 ± SD:0.14) and Pictor (−0.05 ± SD:0.16). Diagnostic sensitivities were highest for Zeiss (84.9%; 95% CI, 78.2–91.5%) followed by Pictor (78.1%; 95% CI, 66.6–89.5%) and Remidio (77.5%; 95% CI, 65.9–89.0%).

Conclusions
Remidio and Pictor achieve comparable results to the Zeiss table-top camera. Both devices achieved similar scores in feasibility, image quality, image gradeability and diagnostic sensitivity. This suggests that these devices potentially offer a more cost-effective alternative in certain clinical scenarios.
QTL-seq for the identification of candidate genes for days to flowering and leaf shape in pigeonpea
Authors: Singh Vikas , Sinha Pallavi , Obala Jimmy , Khan Aamir W. , Chitikineni Annapurna , Saxena Rachit K. , Varshney Rajeev K. 
Published: January 2022

AbstractTo identify genomic segments associated with days to flowering (DF) and leaf shape in pigeonpea, QTL-seq approach has been used in the present study. Genome-wide SNP profiling of extreme phenotypic bulks was conducted for both the traits from the segregating population (F2) derived from the cross combination- ICP 5529 × ICP 11605. A total of 126.63 million paired-end (PE) whole-genome resequencing data were generated for five samples, including one parent ICP 5529 (obcordate leaf and late-flowering plant), early and late flowering pools (EF and LF) and obcordate and lanceolate leaf shape pools (OLF and LLS). The QTL-seq identified two significant genomic regions, one on CcLG03 (1.58 Mb region spanned from 19.22 to 20.80 Mb interval) for days to flowering (LF and EF pools) and another on CcLG08 (2.19 Mb region spanned from 6.69 to 8.88 Mb interval) for OLF and LLF pools, respectively. Analysis of genomic regions associated SNPs with days to flowering and leaf shape revealed 5 genic SNPs present in the unique regions. The identified genomic regions for days to flowering were also validated with the genotyping-by-sequencing based classical QTL mapping method. A comparative analysis of the identified seven genes associated with days to flowering on 12 Fabaceae genomes, showed synteny with 9 genomes. A total of 153 genes were identified through the synteny analysis ranging from 13 to 36. This study demonstrates the usefulness of QTL-seq approach in precise identification of candidate gene(s) for days to flowering and leaf shape which can be deployed for pigeonpea improvement.
Postmortem high-dimensional immune profiling of severe COVID-19 patients reveals distinct patterns of immunosuppression and immunoactivation
Authors: Wu Haibo , He Peiqi , Ren Yong , Xiao Shiqi , Wang Wei , Liu Zhenbang , Li Heng , Wang Zhe , Zhang Dingyu , Cai Jun , Zhou Xiangdong , Jiang Dongpo , Fei Xiaochun , Zhao Lei , Zhang Heng , Liu Zhenhua , Chen Rong , Li Weiqing , Wang Chaofu , Zhang Shuyang , Qin Jiwei , Nashan Björn , Sun Cheng 
Published: January 2022

AbstractA complete diagnostic autopsy is the gold-standard to gain insight into Coronavirus disease 2019 (COVID-19) pathogenesis. To delineate the in situ immune responses to SARS-CoV-2 viral infection, here we perform comprehensive high-dimensional transcriptional and spatial immune profiling in 22 COVID-19 decedents from Wuhan, China. We find TIM-3-mediated and PD-1-mediated immunosuppression as a hallmark of severe COVID-19, particularly in men, with PD-1+ cells being proximal rather than distal to TIM-3+ cells. Concurrently, lymphocytes are distal, while activated myeloid cells are proximal, to SARS-CoV-2 viral antigens, consistent with prevalent SARS-CoV-2 infection of myeloid cells in multiple organs. Finally, viral load positively correlates with specific immunosuppression and dendritic cell markers. In summary, our data show that SARS-CoV-2 viral infection induces lymphocyte suppression yet myeloid activation in severe COVID-19, so these two cell types likely have distinct functions in severe COVID-19 disease progression, and should be targeted differently for therapy.
Morphodynamic limits to environmental signal propagation across landscapes and into strata
Authors: Toby Stephan C. , Duller Robert A. , De Angelis Silvio , Straub Kyle M. 
Published: January 2022

AbstractThe sedimentary record contains unique information about landscape response to environmental forcing at timescales that far exceed landscape observations over human timescales. However, stochastic processes can overprint and shred evidence of environmental signals, such as sediment flux signals, and so inhibit their transfer to strata. Our community currently lacks a quantitative framework to differentiate between environmental signals and autogenic signals in field-scale analysis of strata. Here we develop a framework and workflow to estimate autogenic thresholds for ancient sediment routing systems. Crucially these thresholds can be approximated using measurements that are readily attainable from field systems, circumventing the low temporal resolution offered by strata. This work demonstrates how short-term system dynamics can be accessed from ancient sediment routing systems to place morphodynamic limits on environmental signal propagation across ancient landscapes and into strata.
Genetic diversity in terrestrial subsurface ecosystems impacted by geological degassing
Authors: Bornemann Till L. V. , Adam Panagiotis S. , Turzynski Victoria , Schreiber Ulrich , Figueroa-Gonzalez Perla Abigail , Rahlff Janina , Köster Daniel , Schmidt Torsten C. , Schunk Ralf , Krauthausen Bernhard , Probst Alexander J. 
Published: January 2022

AbstractEarth’s mantle releases 38.7 ± 2.9 Tg/yr CO2along with other reduced and oxidized gases to the atmosphere shaping microbial metabolism at volcanic sites across the globe, yet little is known about its impact on microbial life under non-thermal conditions. Here, we perform comparative metagenomics coupled to geochemical measurements of deep subsurface fluids from a cold-water geyser driven by mantle degassing. Key organisms belonging to uncultivatedCandidatusAltiarchaeum show a global biogeographic pattern and site-specific adaptations shaped by gene loss and inter-kingdom horizontal gene transfer. Comparison of the geyser community to 16 other publicly available deep subsurface sites demonstrate a conservation of chemolithoautotrophic metabolism across sites. In silico replication measures suggest a linear relationship of bacterial replication with ecosystems depth with the exception of impacted sites, which show near surface characteristics. Our results suggest that subsurface ecosystems affected by geological degassing are hotspots for microbial life in the deep biosphere.