bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitric oxide metabolites: associations with cardiovascular biomarkers and clinical parameters in patients with HFpEF
Authors: Piatek Karsten , Feuerstein Anna , Zach Veronika , Rozados da Conceicao Cristina , Beblo Amelie , Belyavskiy Evgeny , Pieske‐Kraigher Elisabeth , Krannich Alexander , Schwedhelm Edzard , Hinz Sarah , Pieske Burkert , Edelmann Frank 
Published: August 2022

AbstractAimsHeart failure with preserved ejection fraction (HFpEF) is one of the most rapidly growing cardiovascular health burden worldwide, but there is still a lack of understanding about the HFpEF pathophysiology. The nitric oxide (NO) signalling pathway has been identified as a potential key element. The aim of our study was to investigate markers of NO metabolism [l‐arginine (l‐Arg), homoarginine (hArg), and asymmetric and symmetric dimethylarginine (ADMA and SDMA)], additional biomarkers [N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP), endothelin‐1 (ET‐1), mid‐regional pro‐adrenomedullin (MR‐proADM), copeptin, and high‐sensitivity C‐reactive protein (hsCRP)], and the endothelial function in an integrated approach focusing on associations with clinical characteristics in patients with HFpEF.Methods and resultsSeventy‐three patients, prospectively enrolled in the ‘German HFpEF Registry’, were analysed. Inclusion criteria were left ventricular ejection fraction (LVEF) ≥ 50%; New York Heart Association functional class ≥ II; elevated levels of NT‐proBNP > 125 pg/mL; and at least one additional criterion for structural heart disease or diastolic dysfunction. All patients underwent transthoracic echocardiography, cardiopulmonary exercise testing, and pulse amplitude tonometry (EndoPAT™). Patients were categorized in two groups based on their retrospectively calculated HFA‐PEFF score. Serum concentrations of l‐Arg, hArg, ADMA, SDMA, NT‐proBNP, ET‐1, MR‐proADM, copeptin, and hsCRP were determined. Patients had a median age of 74 years, 47% were female, and median LVEF was 57%. Fifty‐two patients (71%) had an HFA‐PEFF score ≥ 5 (definitive HFpEF), and 21 patients (29%) a score of 3 to 4 (risk for HFpEF). Overall biomarker concentrations were 126 ± 32 μmol/L for l‐Arg, 1.67 ± 0.55 μmol/L for hArg, 0.74 (0.60;0.85) μmol/L for SDMA, and 0.61 ± 0.10 μmol/L for ADMA. The median reactive hyperaemia index (RHI) was 1.55 (1.38;1.87). SDMA correlated with NT‐proBNP (r = 0.291; P = 0.013), ET‐1 (r = 0.233; P = 0.047), and copeptin (r = 0.381; P = 0.001). ADMA correlated with ET‐1 (r = 0.250; P = 0.033) and hsCRP (r = 0.303; P = 0.009). SDMA was associated with the left atrial volume index (β = 0.332; P = 0.004), also after adjustment for age, sex, and comorbidities. Biomarkers were non‐associated with the RHI. A principal component analysis revealed two contrary clusters of biomarkers.ConclusionsOur findings suggest an impaired NO metabolism as one possible key pathogenic determinant in at least a subgroup of patients with HFpEF. We argue for further evaluation of NO‐based therapies. Upcoming studies should clarify whether subgroups of HFpEF patients can take more benefit from therapies that are targeting NO metabolism and pathway.
Supramolecular Catalysts for Organic Synthesis: Preparation and Applications of Cyclodextrins and Calixarenes in C−C Cross‐Coupling Reactions
Authors: Lima Carolina G. S. , Pauli Fernanda P. , Madriaga Vinicius G. , Amaral Adriane A. P. , Graciano Isabela A. , Meira Vinicius L. , da S. M. Forezi Luana , Ferreira Vitor F. , de M. Lima Thiago , de Carvalho da Silva Fernando 
Published: August 2022

AbstractReactions that promote the formation of carbon–carbon bonds are of great importance in organic synthesis, since they transform available, relatively inexpensive, and small organic molecules into more complex ones. One of the main protocols used for these transformations are cross‐coupling reactions, and due to their versatility and applications, several efforts have been devoted recently to developing catalysts for such reactions. Supramolecular chemistry has become a major player in this field and encompasses approaches that range from the synthesis of intricate complex architectures that may mimic enzymes, the slight structural modification of commercially available scaffolds, or even repurposing highly available materials such as agarose. This Review discusses the development of supramolecular catalysts for cross‐coupling reactions, focusing mostly on the Suzuki, Heck and Sonogashira reactions promoted by such assemblies.
Convergent validity of the central sensitization inventory in women with fibromyalgia: Association with clinical, psychological and psychophysical outcomes
Authors: Valera‐Calero Juan Antonio , Úbeda‐D'Ocasar Edurne , Arias‐Buría José Luis , Fernández‐de‐las‐Peñas César , Gallego‐Sendarrubias Gracia María , Cigarán‐Méndez Margarita 
Published: August 2022

AbstractBackgroundTo assess the relationship between demographic, clinical, psychological and pressure pain sensitivity outcomes with the central sensitization inventory (CSI) in female with fibromyalgia (FMS).MethodsOne hundred and twenty‐six (n = 126) females with FMS completed demographic (age, body mass index, height, weight), clinical (pain history, pain intensity at rest and during daily living activities), psychological (depression/anxiety levels) outcomes and widespread pressure pain sensitivity as well as the central sensitization inventory (CSI). After conducting a multivariable correlation analysis to identify the association between variables, a multiple linear regression model was performed to identify CSI predictors.ResultsThe CSI was negatively associated with age (r = −0.262) and PPTs (r ranged from −0.221 to −0.372) and positively associated with anxiety (r = 0.541), depression (r = 0.415), mean intensity (r = 0.305), worst pain (r = 0.249) and pain during daily living activities (r = 0.398). The stepwise regression analysis revealed that 47.4% of CSI variance in this sample was explained by anxiety levels (27.8%), PPT at greater trochanter (10.5%), age (1.4%), years with pain (4.8%) and pain during daily living activities (2.9%).ConclusionThe current study found that age, pain intensity at rest and pain during daily living activities, anxiety levels and pressure pain sensitivity are associated with the CSI (associated sensitization symptoms) in women with FMS.SignificanceThis study found that sensitization‐associated symptoms in women with FMS are partially influenced by age, pain intensity at rest and pain during daily living activities, anxiety levels and pressure pain sensitivity.
A secure IoT based healthcare framework using modified RSA algorithm using an artificial hummingbird based CNN
Authors: Jacob T. Prem , Pravin A. , Kumar R. Raja 
Published: August 2022

AbstractRecently, a wide variety of real‐world problems are solved via cloud computing, deep learning, machine learning, artificial intelligence, and the Internet of Things (IoT). These methodologies are concerned with different areas like smart cities, agriculture, transportation systems, and healthcare systems. The existing researchers focused on the health care monitoring application along with IoT and cloud computing. It met several shortcomings in case of computational complexity, cost, time, and improper health care data storage and so forth. To overcome these challenges, the novel IoT‐enabled secure healthcare monitoring model is proposed in this study. At first, several sensors are deployed in the human body thereby collecting patients' data with respect to vital parameters like body temperature deviation. The patient's health record dataset consists of 10 attributes namely phone numbers, marital status, address, age, name, heartbeat rate, oxygen level, smoking, temperature, and blood pressure. The data size reduction and normalization are performed in the IoT medical sensor dataset which contains the redundant or irrelevant attributes that are eliminated during pre‐processing. The artificial hummingbird (AHB) algorithm‐based convolutional neural network (AHB‐CNN) model performs both feature extraction and classification of cancer disease. The AHB‐CNN model classifies whether the patient is prone to cancer or not based on the sensor input collected. The received results are then sent to the hospital management for analysis. The Rivest‐Shamir‐Adleman (RSA) encryption method is mostly used in this study due to the major benefits it gives in terms of asymmetric encryption, ease of use, simpler deployment, and high security associated with factoring large prime numbers. A modified RSA algorithm is used in this article which uses the double encryption‐decryption process and “n” prime numbers to enhance the security of the conventional RSA algorithm. The data is always encrypted during transit before being stored in the cloud or for further processing. Depending upon the experimental consequences, the proposed method established superior performances compared to other state‐of‐art techniques.
Laparoscopic entry techniques: Which should you prefer?
Authors: Raimondo Diego , Raffone Antonio , Travaglino Antonio , Ferla Stefano , Maletta Manuela , Rovero Giulia , Renzulli Federica , de Laurentiis Umberto , Borghese Giulia , Ambrosio Marco , Salucci Paolo , Casadio Paolo , Mollo Antonio , Seracchioli Renato 
Published: August 2022

AbstractBackgroundDespite a debate spanning two decades, no consensus has been achieved about the safest laparoscopic entry technique.ObjectivesTo update the evidence about the safety of the main different laparoscopic entry techniques.Search StrategySix electronic databases were searched from inception to February 2021.Selection CriteriaAll randomized controlled trials (RCTs) comparing different laparoscopic entry techniques were included.Data Collection and AnalysisEntry‐related complications and total time for entry were compared among the different methods of entry calculating pooled odds ratios (ORs) and mean differences, with 95% confidence intervals (CIs); P < 0.05 was considered significant.Main ResultsIn total, 25 RCTs (6950 patients) were included. Complications considered were vascular, visceral and omental injury, failed entry, extraperitoneal insufflation, bleeding and infection at the trocar site bleeding, and incisional hernia. Compared to direct trocar, the OR for Veress needle was significantly higher for omental injury (OR 3.65, P < 0.001), for failed entry (OR 4.19, P < 0.001), and for extraperitoneal insufflation (OR 5.29, P < 0.001). Compared to the open method, the OR for Veress needle was significantly higher for omental injury (OR 4.93, P = 0.001), for failed entry (OR 2.99, P < 0.001), for extraperitoneal insufflation (OR 4.77; P = 0.04), and for incisional hernia. Compared to the open method, the OR for direct trocar was significantly lower for visceral injury (OR 0.17, P = 0.002) and for trocar site infection (OR 0.27, P = 0.001).ConclusionsThe direct trocar method may be preferred over Veress needle and open methods as a laparoscopic entry technique since it appears associated to a lower risk of complications.
mibPOPdb: An online database for microbial biodegradation of persistent organic pollutants
Authors: Ngara Tanyaradzwa R. , Zeng Peiji , Zhang Houjin 
Published: August 2022

AbstractMicrobial biodegradation of persistent organic pollutants (POPs) is an attractive, ecofriendly, and cost‐efficient clean‐up technique for reclaiming POP‐contaminated environments. In the last few decades, the number of publications documenting POP‐degrading microbes, enzymes, and experimental data sets has continuously increased, necessitating the development of a dedicated web resource that catalogs consolidated information on POP‐degrading microbes and tools to facilitate integrative analysis of POP degradation data sets. To address this knowledge gap, we developed the Microbial Biodegradation of Persistent Organic Pollutants Database (mibPOPdb) by accumulating microbial POP degradation information from the public domain and manually curating published scientific literature. Currently, in mibPOPdb, there are 9215 microbial strain entries, including 184 gene (sub)families, 100 enzymes, 48 biodegradation pathways, and 593 intermediate compounds identified in POP‐biodegradation processes, and information on 32 toxic compounds listed under the Stockholm Convention environmental treaty. Besides the standard database functionalities, which include data searching, browsing, and retrieval of database entries, we provide a suite of bioinformatics services to facilitate comparative analysis of users' own data sets against mibPOPdb entries. Additionally, we built a Graph Neural Network‐based prediction model for the biodegradability classification of chemicals. The predictive model exhibited a good biodegradability classification performance and high prediction accuracy. mibPOPdb is a free data‐sharing platform designated to promote research in microbial‐based biodegradation of POPs and fills a long‐standing gap in environmental protection research. Database URL: http://mibpop.genome-mining.cn/
PEX1 is a mediator of α1‐adrenergic signaling attenuating doxorubicin‐induced cardiotoxicity
Authors: Li Wenjuan , Xie Huilin , Hu Huang , Huang Jihong , Chen Sun 
Published: August 2022

AbstractDoxorubicin (DOX) is a potent chemotherapeutic agent used for cancer treatment, however, DOX‐induced cardiotoxicity is a serious clinical problem because it causes acute and chronic heart dysfunction. Many studies have indicated that the α1‐adrenergic receptor protects the heart from pathologic stress through activation survival signaling, however, the mechanism remains largely unknown. Previous studies have detected that the phenylephrine‐induced complex‐1 (PEX1) transcription factor, also known as zinc‐finger protein 260 (Zfp260), is an effector of α1‐adrenergic signaling in cardiac hypertrophy. Our present study aimed to investigate the role and underlying mechanism of PEX1 in cardiomyocyte survival during DOX‐induced cardiotoxicity. Mice were exposed to a single intraperitoneal injection of DOX (15 mg/kg) to generate DOX‐induced cardiotoxicity. We found that PEX1 expression was downregulated in DOX‐treated murine hearts. PEX1 deficiency resulted in increased apoptosis, and conversely, PEX1 overexpression alleviated apoptosis induced by DOX in primary cardiomyocytes, as well as upregulated antiapoptotic genes such as BCL‐2 and BCL‐XL. Mechanistically, we identified that PEX1 might exert its antiapoptosis effect by playing a pivotal role in the action of α1‐adrenergic signaling activation, which depends on the presence of GATA‐4. Based on these findings, we supposed that PEX1 may be a novel transcription factor involved in cardiac cell survival and a promising candidate target for DOX‐induced cardiotoxicity.
Tau liquid–liquid phase separation: At the crossroads of tau physiology and tauopathy
Authors: Islam Majedul , Shen Fengyun , Regmi Deepika , Petersen Katherine , Karim Md Raza Ul , Du Deguo 
Published: August 2022

AbstractAbnormal deposition of tau in neurons is a hallmark of Alzheimer's disease and several other neurodegenerative disorders. In the past decades, extensive efforts have been made to explore the mechanistic pathways underlying the development of tauopathies. Recently, the discovery of tau droplet formation by liquid–liquid phase separation (LLPS) has received a great deal of attention. It has been reported that tau condensates have a biological role in promoting and stabilizing microtubule (MT) assembly. Furthermore, it has been hypothesized that the transition of phase‐separated tau droplets to a gel‐like state and then to fibrils is associated with the pathology of neurodegenerative diseases. In this review, we outline LLPS, the structural disorder that facilitates tau droplet formation, the effects of posttranslational modification of tau on condensate formation, the physiological function of tau droplets, the pathways from droplet to toxic fibrils, and the therapeutic strategies for tauopathies that might evolve from toxic droplets. We expect a deeper understanding of tau LLPS will provide additional insights into tau physiology and tauopathies.
Concordance between the BD Onclarity and Roche cobas assays for detection of HPV DNA in a Chinese population
Authors: Wang Yakun , Li Tingyuan , Yin Jian , Liu Yin , Li Zhifang , Liu Yujing , Chen Tingting , Chen Simiao , Dai Yu , Cui Jianfeng , Liu Bin , Feng Xiangxian , Zhang Shaokai , Chen Wen 
Published: August 2022

AbstractAs cervical cancer screening shifts from cytology to human papillomavirus (HPV) testing, a major issue involves validating more HPV tests. In recent years, some HPV tests are used for clinical performance verification in China. The purpose of this study was to explore whether the BD Onclarity (Becton, Dickinson and Company)HPV assay differs from the Roche cobas (Roche Molecular Systems)HPV assay, as determined using 944 cervical samples, including 588 with sequencing results. In the nucleic acid assay accuracy verification, the assays showed excellent concordance for detection of HPV16 (κ = 0.93, 95% confidence interval [CI]: 0.89–0.97) and HPV18 (κ = 0.90, 95% CI: 0.83–0.97), and very good concordance for the 12 other high‐risk types (HPV31/33/35/39/45/51/52/56/58/59/66/68, κ = 0.79, 95% CI: 0.75–0.83). The overall agreement for HPV DNA detection between Onclarity and cobas was very good (κ = 0.7755). No difference for ≥CIN2 sensitivity was observed between Onclarity and cobas (both 96.5%), whereas the ≥CIN2 specificity for detection of Onclarity (16.6%, 95% CI: 13.7–19.9) was higher than that of cobas (11.5%, 95% CI: 9.1–14.5). Onclarity exhibited comparable screening performance and triage efficiency compared to cobas in the detection of cervical disease in Chinese women.
The Late Pleistocene megafauna of the Chulym River basin, southeastern West Siberian Plain: chronology and stable isotope composition
Authors: Kuzmin Yaroslav V. , Shpansky Andrei V. 
Published: August 2022

AbstractNew data were obtained for the Chulym River basin in the southeastern part of the West Siberian Plain, one of the understudied parts of Siberia in terms of age and composition of carbon and nitrogen stable isotopes for Late Pleistocene megafauna. The 14C dates from the Sergeevo outcrop, the most complete section of Late Quaternary deposits in the region, are mostly greater than ~30 550 bp. Other localities yielded 14C values in the range from >44 500 to ~19 300 bp. The finite date of ~42 270 bp for the Khozarian steppe elephant (Mammuthus trogontherii chosaricus) from Asino is intriguing because previously it was not detected in the Late Pleistocene of Siberia after the last interglacial (Marine Isotope Stage 5e), ~115 000–130 000 years ago. Stable isotope data show both similarities and differences compared to the pre‐Last Glacial Maximum megafaunal species in other parts of Siberia.
Clinical outcomes of patients with Liver Imaging Reporting and Data System 3 or Liver Imaging Reporting and Data System 4 observations in patients with cirrhosis: A systematic review
Authors: Kanneganti Mounika , Marrero Jorge A. , Parikh Neehar D. , Kanwal Fasiha , Yokoo Takeshi , Mendiratta‐Lala Mishal , Rich Nicole E. , Gopal Purva , Singal Amit G. 
Published: August 2022

Abstract
Patients with indeterminate liver nodules, classified as LR‐3 and LR‐4 observations per the Liver Imaging Reporting and Data System, are at risk of developing hepatocellular carcinoma (HCC), but risk estimates remain imprecise. We conducted a systematic review of Ovid MEDLINE, EMBASE, and Cochrane databases from inception to December 2021 to identify cohort studies examining HCC incidence among patients with LR‐3 or LR‐4 observations on computed tomography (CT) or magnetic resonance imaging (MRI). Predictors of HCC were abstracted from each study, when available. Of 13 total studies, nine conducted LR‐3 observation‐level analyses, with the proportions of incident HCC ranging from 1.2% to 12.5% at 12 months and 4.2% to 44.4% during longer study follow‐up. Among three studies with patient‐level analyses, 8%–22.2% of patients with LR‐3 lesions developed LR‐4 observations and 11.1%–24.5% developed HCC. Among nine studies conducting LR‐4 observation‐level analyses, incident HCC ranged from 30.8% to 44.0% at 12 months and 30.9% to 71.0% during study follow‐up; conversely, 6%–42% of observations were downgraded to LR‐3 or lower. Patient‐level factors associated with HCC included older age, male sex, higher alpha‐fetoprotein levels, viral etiology, and prior history of HCC; observation‐level factors included maximum diameter, threshold growth, T2 hyperintensity, and visibility on ultrasound. Studies were limited by small sample sizes, inclusion of patients with prior HCC, short follow‐up duration, and failure to account for clustering of observations in patients or competing risks of transplantation and death. LR‐3 and LR‐4 observations have elevated but variable risks of HCC. Higher quality studies are necessary to identify high‐risk patients who warrant close CT or MRI‐based follow‐up.
Botulinum toxin A injection to the external urethral sphincter for voiding dysfunction in females: A tertiary center experience
Authors: Nadeem Mehwash , Lindsay Jamie , Pakzad Mahreen , Hamid Rizwan , Ockrim Jeremy , Greenwell Tamsin 
Published: August 2022

AbstractAimThe aim of this study is to examine the functional outcomes of ona‐botulinum toxin A (BTX‐A) injection into the external urethral sphincter (EUS) for female patients with nonneurogenic nonrelaxing sphincter as the underlying cause of voiding dysfunction (VD).METHODA retrospective analysis was performed for all the patients with the urodynamic findings of higher than expected maximum urethral closure pressure (MUCP) who received their first injection during the study period. All patients were evaluated with preoperative videourodynamic study and urethral pressure profilometry and received 100 U of EUS BTX‐A. Patients aged less than 18 years and those with neurogenic bladder were excluded. All patients were followed up with the free flow, postvoid residuals (PVR), and patient global impression of improvement (PGI‐I) scale at 6 weeks and then at 3 monthly intervals.ResultWe identified 35 female patients with a mean age of 37.5 ± 15 years (range 18–72 years) with a mean follow‐up of 20 months. More than 50% of patients had a history of prior surgical intervention and 28 (80%) patients were catheter dependent, a suprapubic catheterization, or clean intermittent self‐catheterization. Mean MUCP was 97.1 ± 22 cm of water. After treatment with BTX‐A, 21 (60%) patients were able to void per urethral (p = 0.02). The mean maximum flow rate (Qmax) improved from 8.8 to 11 mls/s and the mean PVR decreased from 200 to 149 mls (p < 0.05). On multivariate analysis, we identified high preoperative PVR, high preoperative actual MUCP, and previous surgical intervention (urethral dilatation, sacral neuromodulation, and pelvic surgery) as predictors of successful voiding restoration. The mean duration of response was 4.7 months, 46% of patients requested repeat injection, and 29% were established on maintenance injections. On the 5‐point PGI‐I score, 13 (37%), 12 (34%), and 10 (29%) patients reported good, some, and no improvement, respectively. Quality of life was also improved in 60% of patients. Two patients had transient stress urinary incontinence (for <6 weeks) and there were no significant long‐lasting adverse events.ConclusionEUS BTX‐A is a valid treatment option for VD considering therapeutic options are limited. The patient must be made aware of the need for repeat treatments.