bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative suitability evaluation and spatial zoning for seawater desalination water intake site selection
Authors: Huang Pei , Sun Yonggen , Gu Donggi , Yan Wenwen , Chen Yong , Wang Enkang 
Published: November 2022

Seawater desalination water intake site selection has an important impact on the investment, water production cost, stable operation, and safety of seawater desalination plants. Site selection is affected by many factors, such as the natural geography, ecological environment, and social economy of coastal zones; some constraints can be directly identified as unsuitable areas for these construction projects. For the shallow water intake method of seawater desalination, this study selects suitability evaluation indicators for seawater desalination water intake site selection from the three influencing factors of basic geography, water environment, and industrial development and constructs a suitability evaluation model based on the multifactor spatial overlay analysis of the Geographic Information System platform. This model carries out a quantitative suitability evaluation of the seawater desalination water intake site selection and realizes the suitable spatial zoning for spatially selecting the water intake, thus forming a scientific, quantitative, and spatial suitability evaluation system for this process. The evaluation method was applied in the Rongcheng city offshore area of China and analyzed. The evaluation results showed that the suitable areas for seawater desalination water intake site selection comprised 304.8 square kilometers, which were mainly distributed in the offshore areas in northern Rongcheng city and near the coastline areas of its central and southern regions. The unsuitable areas covered 292.4 square kilometers, mainly distributed in the marine ecological red line areas and the coastal aquaculture areas of Rongcheng city. The evaluation results met the site selection needs of the seawater desalination water intake project in Rongcheng city. This study improves upon the existing method of seawater desalination intake site selection at the theoretical and technical levels and provides a scientific basis for the location selection and planning for water intake in large spatial ranges of coastal waters.
RIPK1 mutations causing infantile-onset IBD with inflammatory and fistulizing features
Authors: Sultan Mutaz , Adawi Mohammad , Kol Nitzan , McCourt Blake , Adawi Ihda , Baram Liran , Tal Noa , Werner Lael , Lev Atar , Snapper Scott B. , Barel Ortal , Konnikova Liza , Somech Raz , Shouval Dror S. 
Published: November 2022

PurposeReceptor-interacting serine/threonine-protein kinase 1 (RIPK1) is an important regulator of necroptosis and inflammatory responses. We present the clinical features, genetic analysis and immune work-up of two patients with infantile-onset inflammatory bowel disease (IBD) resulting from RIPK1 mutations.MethodsWhole exome and Sanger sequencing was performed in two IBD patients. Mass cytometry time of flight (CyTOF) was conducted for in-depth immunophenotyping on one of the patient’s peripheral blood mononuclear cells, and compared to control subjects and patients with Crohn’s disease.ResultsThe patients presented with severe colitis and perianal fistulas in the first months of life, without severe/atypical infections. Genetic studies identified pathogenic genetic variants in RIPK1 (Patient 1, A c.1934C&gt;T missense mutation in Exon 11; Patient 2, c.580G&gt;A missense mutation residing in Exon 4). Protein modeling demonstrated that the mutation in Patient 1 displaces a water molecule, potentially disrupting the local environment, and the mutation in Patient 2 may lead to disruption of the packing and conformation of the kinase domain. Immunofluorescence RIPK1 staining in rectal biopsies demonstrated no expression for Patient 1 and minimal expression for Patient 2, compared to controls and patients with active Crohn’s disease. Using CyTOF unbiased clustering analysis, we identified peripheral immune dysregulation in one of these patients, characterized by an increase in IFNγ CD8+ T cells along with a decrease in monocytes, dendritic cells and B cells. Moreover, RIPK1-deficient patient’s immune cells exhibited decreased IL-6 production in response to lipopolysaccharide (LPS) across multiple cell types including T cells, B cells and innate immune cells.ConclusionsMutations in RIPK1 should be considered in very young patients presenting with colitis and perianal fistulas. Given RIPK1’s role in inflammasome activation, but also in epithelial cells, it is unclear whether IL1 blockade or allogeneic hematopoietic stem cell transplantation can suppress or cure the hyper-inflammatory response in these patients. Additional studies in humans are required to better define the role of RIPK1 in regulating intestinal immune responses, and how treatment can be optimized for patients with RIPK1 deficiency.
Systemic immune-inflammation index is associated with hepatic steatosis: Evidence from NHANES 2015-2018
Authors: Song Yancheng , Guo Wencong , Li Zhaopeng , Guo Dong , Li Zhao , Li Yu 
Published: November 2022

BackgroundAs a novel inflammatory marker, Systemic Immune-Inflammation Index (SII) has not been studied with hepatic steatosis. The aim of this study was to investigate the possible relationship between SII and hepatic steatosis.MethodsIn the cross-sectional investigation, adults having complete information on SII, hepatic steatosis, and bariatric surgery from the 2015–2018 National Health and Nutrition Examination Survey (NHANES) were included. Hepatic steatosis was evaluated with heaptic steatosis index (HSI). The platelet count × neutrophil count/lymphocyte count was used to compute SII. We investigated the independent interaction between SII and hepatic steatosis using weighted multivariable regression analysis and subgroup analysis. To explore the potential relationship between SII, bariatric surgery and hepatic steatosis by controlling potential confounders by propensity score matching.ResultsThe study involved 10505 participants in total, 5937 (56.5%) of whom had hepatic steatosis according to the diagnosis. After adjusted for covariates, multivariable logistic regression revealed that high SII level was an independent risk factor for hepatic steatosis (OR = 1.30, 95% CI: 1.10-1.52, P 0.01). Unexpectedly, bariatric surgery reduced SII even after PSM corrected for differences of BMI and HSI.ConclusionsIn US adults, SII was positively correlated with an increase in hepatic steatosis. The SII may be a simple and affordable way to identify hepatic steatosis. Bariatric surgery may reduce SII without resorting to weight loss. This needs to be verified in additional prospective research.
Divergent dynamics of inflammatory mediators and multiplex PCRs during airway infection in cystic fibrosis patients and healthy controls: Serial upper airway sampling by nasal lavage
Authors: Erdmann Nina , Schilling Theresa , Hentschel Julia , Lehmann Thomas , von Bismarck Philipp , Ankermann Tobias , Duckstein Franziska , Baier Michael , Zagoya Carlos , Mainz Jochen G. 
Published: November 2022

BackgroundIn cystic fibrosis (CF), acute respiratory exacerbations critically enhance pulmonary destruction. Since these mainly occur outside regular appointments, they remain unexplored. We previously elaborated a protocol for home-based upper airway (UAW) sampling obtaining nasal-lavage fluid (NLF), which, in contrast to sputum, does not require immediate processing. The aim of this study was to compare UAW inflammation and pathogen colonization during stable phases and exacerbations in CF patients and healthy controls.MethodsInitially, we obtained NLF by rinsing 10 ml of isotonic saline/nostril during stable phases. During exacerbations, subjects regularly collected NLF at home. CF patients directly submitted one aliquot for microbiological cultures. The remaining samples were immediately frozen until transfer on ice to our clinic, where PCR analyses were performed and interleukin (IL)-1β/IL-6/IL-8, neutrophil elastase (NE), matrix metalloproteinase (MMP)-9, and tissue inhibitor of metalloproteinase (TIMP)-1 were assessed.ResultsAltogether, 49 CF patients and 38 healthy controls (HCs) completed the study, and 214 NLF samples were analyzed. Of the 49 CF patients, 20 were at least intermittently colonized with P. aeruginosa and received azithromycin and/or inhaled antibiotics as standard therapy. At baseline, IL-6 and IL-8 tended to be elevated in CF compared to controls. During infection, inflammatory mediators increased in both cohorts, reaching significance only for IL-6 in controls (p=0.047). Inflammatory responses tended to be higher in controls [1.6-fold (NE) to 4.4-fold (MMP-9)], while in CF, mediators increased only moderately [1.2-1.5-fold (IL-6/IL-8/NE/TIMP-1/MMP-9)]. Patients receiving inhalative antibiotics or azithromycin (n=20 and n=15, respectively) revealed lower levels of IL-1β/IL-6/IL-8 and NE during exacerbation compared to CF patients not receiving those antibiotics. In addition, CF patients receiving azithromycin showed MMP-9 levels significantly lower than CF patients not receiving azithromycin at stable phase and exacerbation. Altogether, rhinoviruses were the most frequently detected virus, detected at least once in n=24 (49.0%) of the 49 included pwCF and in n=26 (68.4%) of the 38 healthy controls over the 13-month duration of the study. Remarkably, during exacerbation, rhinovirus detection rates were significantly higher in the HC group compared to those in CF patients (65.8% vs. 22.4%; p&lt;0.0001).ConclusionNon-invasive and partially home-based UAW sampling opens new windows for the assessment of inflammation and pathogen colonization in the unified airway system.
Nigeria at 62: Quagmire of malaria and the urgent need for deliberate and concerted control strategy
Authors: Oboh Mary A. , Oyebola Kolapo M. , Ajibola Olumide , Thomas Bolaji N. 
Published: November 2022

BackgroundSub-Saharan Africa (SSA) has disproportionately contributed the majority (95%) of all malaria cases and deaths for more than a decade (2010-2021) and Nigeria contributes the highest in global malaria cases and deaths in the last decade.Main bodyDespite several malaria control initiatives, why is Nigeria still the most endemic malaria country? Published reports have underlined possible reasons for the sustenance of malaria transmission. Malaria transmission pattern in the country is largely and remarkably heterogeneous, hence control measures must take this uniqueness into consideration when designing intervention strategies. Nigeria became 62 years post-independence on the 1st of October, 2022, therefore making positive impacts on all aspects of the country, especially in the health sector becomes imperative more than ever before. To achieve a pre-elimination malaria status, we propose the implementation of focused and calculated research strategies. Such strategies would be consciously geared towards understanding vectorial capacity, susceptibility to approved insecticides, identifying malaria hotspots, and deciphering the genetic structure and architecture of P. falciparum within and between groups and regions. This will provide insight into delineating the inter/intra-regional migration of parasite populations, amongst others.ConclusionWith regard to malaria elimination, Nigeria still has a long way to go. There is a need for dedicated prioritization of research efforts that would provide a basic understanding of the Plasmodium parasite in circulation. Such information will support the implementation of policies that will drive down malaria transmission in Nigeria.
Effects of acupuncture on the outcome of tinnitus: An overview of systematic reviews
Authors: Xu Xianpeng , Xie Hui , Liu Zifeng , Guo Tao , Zhang Ying 
Published: November 2022

BackgroundTo systematically summarize the evidence for the efficacy of acupuncture in tinnitus treatment, we assessed the methodological quality, reporting quality, and evidence quality of systematic reviews/meta-analyses (SRs/MAs) of acupuncture in the treatment of tinnitus.MethodsFrom inception to March 2022, we conducted a detailed and comprehensive search of eight electronic databases in Chinese and English. The Assessing the Methodological Quality of Systematic Reviews 2 (AMSTAR-2), the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist, and the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) were used to assess methodological quality, reporting quality and evidence quality for inclusion in SRs/MAs, respectively.ResultsFourteen published SRs/MAs met the inclusion criteria and were included in the study. Eleven studies reported that acupuncture was effective in treating tinnitus, and three studies reported that no firm conclusions could be drawn about the effectiveness of acupuncture in treating tinnitus. The results of the AMSTAR-2 assessment showed that the methodological quality of the included studies was relatively low in general, with one being moderate quality and the rest being very low quality. The PRISMA checklist evaluation results showed that no studies fully report checklists, with protocol registration and search strategies being the main reporting weaknesses. The GRADE assessment showed that no results were high-quality evidence, 17 results were moderate-quality evidence, 25 results were low-quality evidence, and 12 results were very low-quality evidence.ConclusionAcupuncture seems to be a positive and effective treatment for tinnitus. However, the methodological quality and quality of evidence for SRs/MAs in the included studies were generally low, and this result must be viewed with caution. Therefore, more high-quality, large-scale, multi-center randomized controlled trials are needed in the future to verify the effectiveness of acupuncture in the treatment of tinnitus.
The relationship between aesthetic preferences of people for ceramic tile design and neural responses: An event-related potential study
Authors: Chen Jiayin , Cheng Yue 
Published: November 2022

IntroductionThe aesthetic preferences of people can determine the success of a design and are often closely related to design features. The discovery of designs that match user preferences can provide a reference for designers. Ceramic tiles are widely used in environmental design; however, little attention has been paid to the aesthetic preferences of people for tiles. This study aimed to explore the relationship between aesthetic preferences for tile design and neural responses.Materials and methodsIn this study, two groups of tiles with different preference levels were randomly presented to 16 participants, and their electroencephalograms were recorded. The mean amplitudes of event-related potentials were analyzed by ANOVA.ResultsThe results showed that: (1) the aesthetic preferences of people for tiles could modulate brain activity; (2) tiles that people liked triggered higher N100 amplitudes; and (3) tiles that people disliked triggered higher P200 and late positive potential (LPP) amplitudes.DiscussionThese results suggest that N100, P200, and LPP are significantly related to the aesthetic preferences of people for ceramic tiles. The difference in N100 and P200 amplitudes indicates that participants developed aesthetic perceptions of the tiles in the early and middle stages of vision and formed different attention allocations to tiles with varying levels of aesthetic preference; in the middle and late stages of visual processing, the difference in the LPP amplitude indicates that the impression of people for tiles is further deepened in the later stage, forming a top-down emotion-driven evaluation. Exploring the relationship between the aesthetic preferences of people and neural responses is significant in establishing objective aesthetic judgment indicators for tiles and understanding the process of aesthetic cognition. This study provides relevant information for quantitative aesthetic assessments of environmental design, interior design, and marketing involving ceramic tiles.
Resting-state electroencephalogram microstate to evaluate post-stroke rehabilitation and associate with clinical scales
Authors: Wang Zhongpeng , Liu Zhaoyang , Chen Long , Liu Shuang , Xu Minpeng , He Feng , Ming Dong 
Published: November 2022

IntroductionStroke is usually accompanied by a range of complications, like post-stroke motor disorders. So far, its evaluation of motor function is developed on clinical scales, such as Fugl-Meyer Assessment (FMA), Instrumental Activities of Daily Living (IADL), etc. These scale results from behavior and kinematic assessment are inevitably influenced by subjective factors, like the experience of patients and doctors, lacking neurological correlations and evidence.MethodsThis paper applied a microstate model based on modified k-means clustering to analyze 64-channel electroencephalogram (EEG) from 12 stroke patients and 12 healthy volunteers, respectively, to explore the feasibility of applying microstate analysis to stroke patients. We aimed at finding some possible differences between stroke and healthy individuals in resting-state EEG microstate features. We further explored the correlations between EEG microstate features and scales within the stroke group.Results and discussionBy statistical analysis, we obtained significant differences in EEG microstate features between the stroke and healthy groups and significant correlations between microstate features and scales within the stroke group. These results might provide some neurological evidence and correlations in the perspective of EEG microstate analysis for post-stroke rehabilitation and evaluation of motor disorders. Our work suggests that microstate analysis of resting-state EEG is a promising method to assist clinical and assessment applications.
The role of [18F]FDG-PET/CT in gram-positive and gram-negative bacteraemia: A systematic review
Authors: Packham Alice , Spence Niamh , Bawa Tanveer , Srinivasan Rohit , Goodman Anna L. 
Published: November 2022

ObjectivesBacteraemia is associated with significant morbidity and mortality. [18F]FDG-PET/CT is increasingly used to detect infectious metastatic foci, however there remains international variation in its use. We performed a systematic review assessing the impact of [18F]FDG-PET/CT in adult inpatients with gram-positive and Gram-negative bacteraemia.DesignThe systematic review was performed according to PRISMA guidelines. Studies published between 2009 and December 2021 were searched in MEDLINE, EMBASE and Cochrane clinical trials database. Data extraction and quality assessment was performed using ROBINS-I and GRADE.SettingEligible study designs included randomised-controlled trials, clinically-controlled trials, prospective trials, retrospective trials, case-control studies, and non-controlled studies.ParticipantsStudies solely assessing adult inpatients with blood-culture confirmed bacteraemia with one cohort of patients receiving [18F]FDG-PET/CT were included.Main outcome measuresprimary outcomes were mortality, identification of metastatic foci and relapse rate. Studies not examining any of the pre-specified outcomes were excluded.ResultsTen studies were included, of which five had a non-PET/CT control arm. Overall, there was low quality of evidence that [18F]FDG-PET/CT is associated with reduced mortality, improved identification of metastatic foci and reduced relapse rate. Six studies assessed Staphylococcus aureus bacteraemia (SAB) only; nine studies included Gram-positive bacteraemia only, and one study included data from Gram-negative bacteraemia. Two studies compared outcomes between patients with different types of bacteraemia. Four studies identified a statistically significant difference in mortality in [18F]FDG-PET/CT recipients and controls. Relapse rate was significantly reduced in patients with SAB who received [18F]FDG-PET/CT. Studies identified significantly higher detection of metastatic foci in [18F]FDG-PET/CT recipients compared to controls. [18F]FDG-PET/CT was the first to identify an infectious site in 35.5% to 67.2% of overall foci identified.ConclusionsFurther research is required to establish the role of [18F]FDG-PET/CT in bacteraemia, and its impact on management and mortality.
The glucotoxicity protecting effect of honokiol in human hepatocytes via directly activating AMPK
Authors: Liu Hui , Luo Wu , Liu Jiazheng , Kang Xincong , Yan Jianming , Zhang Tingting , Yang Lan , Shen Lu , Liu Dongbo 
Published: November 2022

IntroductionSustained hyperglycemia causes glucotoxicity, which has been regarded as a contributor to hepatocyte damage in type 2 diabetes (T2D) and its metabolic comorbidities. Honokiol is a natural biphenolic component derived from the dietary supplement Magnolia officinalis extract. This study aimed to investigate the effects of honokiol on glucose metabolism disorders and oxidative stress in hepatocytes and the underlying mechanisms.MethodsHepG2 cells were treated with glucosamines (18 mM) to induce glucotoxicity as a diabetic complication model in vitro.Results and discussionHonokiol significantly increased glucose consumption, elevated 2-NBDG uptake, and promoted GLUT2 translocation to the plasma membrane in glucosamine-treated HepG2 cells, indicating that honokiol ameliorates glucose metabolism disorders. Furthermore, glucosamine-induced ROS accumulation and loss of mitochondrial membrane potential were markedly reduced by honokiol, suggesting that honokiol alleviated glucotoxicity-induced oxidative stress. These effects were largely abolished by compound C, an AMPK inhibitor, suggesting an AMPK activation-dependent manner of honokiol function in promoting glucose metabolism and mitigating oxidative stress. Molecular docking results revealed that honokiol could interact with the amino acid residues (His151, Arg152, Lys243, Arg70, Lys170, and His298) in the active site of AMPK. These findings provide new insights into the antidiabetic effect of honokiol, which may be a promising agent for the prevention and treatment of T2D and associated metabolic comorbidities.