bluuebunny/crossref_metadata_2025_split huggingface dataset:
PLEK2 promotes cancer stemness and tumorigenesis of head and neck squamous cell carcinoma via the c‐Myc‐mediated positive feedback loop
Authors: Zhao Xinyuan , Shu Dalong , Sun Wenjuan , Si Shanshan , Ran Wei , Guo Bing , Cui Li 
Published: August 2022

AbstractBackgroundHead and neck squamous cell carcinoma (HNSCC) is one of the most frequent malignancies worldwide and is characterized by unfavorable prognosis, high lymph node metastasis and early recurrence. However, the molecular events regulating HNSCC tumorigenesis remain poorly understood. Therefore, uncovering the underlying mechanisms is urgently needed to identify novel and promising therapeutic targets for HNSCC. In this study, we aimed to explore the role of pleckstrin‐2 (PLEK2) in regulating HNSCC tumorigenesis.MethodsThe expression pattern of PLEK2 and its clinical significance in HNSCC were determined by analyzing publicly assessable datasets and our own independent HNSCC cohort. In vitro and in vivo experiments, including cell proliferation, colony formation, Matrigel invasion, tumor sphere formation, ALDEFLUOR, Western blotting assays and xenograft mouse models, were used to investigate the role of PLEK2 in regulating the malignant behaviors of HNSCC cells. The underlying molecular mechanisms for the tumor‐promoting role of PLEK2 were elucidated using co‐immunoprecipitation, cycloheximide chase analysis, ubiquitination assays, chromatin immunoprecipitation‐quantitative polymerase chain reaction, luciferase reporter assays and rescue experiments.ResultsThe expression levels of PLEK2 mRNA and protein were significantly increased in HNSCC tissues, and PLEK2 overexpression was strongly associated with poor overall survival and therapeutic resistance. Additionally, PLEK2 was important for maintaining the proliferation, invasion, epithelial‐mesenchymal transition, cancer stemness and tumorigenesis of HNSCC cells and could alter the cellular metabolism of the cancer cells. Mechanistically, PLEK2 interacted with c‐Myc and reduced the association of F‐box and WD repeat domain containing 7 (FBXW7) with c‐Myc, thereby avoiding ubiquitination and subsequent proteasome‐mediated degradation of c‐Myc. Moreover, the c‐Myc signaling activated by PLEK2 was important for sustaining the aggressive malignant phenotypes and tumorigenesis of HNSCC cells. c‐Myc also directly bounded to the PLEK2 promoter and activated its transcription, forming a positive feedback loop.ConclusionsCollectively, these findings uncover a previously unknown molecular basis of PLEK2‐enhanced c‐Myc signaling in HNSCC, suggesting that PLEK2 may represent a promising therapeutic target for treating HNSCC.
Nanoarchitectonics with Positionally Isomeric Coumarin Carbamates: Structure‐Gelation Study, F− Recognition, Dye Removal and Excellent Oil‐Spill Recovery
Authors: Raza Rameez , Baildya Nabajyoti , Ghosh Kumaresh 
Published: August 2022

AbstractCoumarin carbamates with cholesteryl group 1 and 2 have been designed, synthesized and characterized. The isomeric compounds show different gelation behaviors in organic solvents such as toluene and CH3CN. Rheological studies reveal the different viscoelastic properties of the gels. Gels of the isomers differ in morphology. While an intertwined fibre‐like structure is noted for the toluene gel of 1, rod like fibres are observed for toluene gel of 2. The positional alteration of carbamate group around coumarin changes the polarity of the isomers and hence influences the packing of molecules to establish different 3D networks for gel formation as explained by DFT study. Gels of the isomers show multiple applications with different attributes. Toluene gel of 1 is found to be F−‐responsive and undergoes gel‐to‐sol phase transition due to the disruption of intermolecular hydrogen bonding through deprotonation. Under the condition, toluene gel of 2 is anion stable. Compound 1 also shows measurable interaction with F− over other anions in CH3CN as interpreted by UV‐vis and emission spectral changes. Based on phase‐selective gelation (PSG) property, both 1 and 2 is deployed to remove cationic dye from water. Further, only gelator 2 is useful for oil spill recovery, whereas isomer 1 is not suitable in this regard.
Tuning the Emission Behaviour of Halogenated Bridged Ethers in Solution, as Solids and as Aggregates by Chalcogen Substitution
Authors: Dubbert Justin , Valtolina Marco , Huber Alexander , Scherz Tim D. , Wölper Christoph , Daniliuc Constantin G. , Filiba Ofer , Sen Saumik , Schapiro Igor , Rizzo Fabio , Voskuhl Jens 
Published: August 2022

AbstractIn this contribution, we describe a set of three chlorinated bridged ethers with varying numbers of sulfur and oxygen atoms. The substitution leads to highly emissive compounds with tunable photophysical properties in relationship to their state of aggregation, i. e. in solution, as aggregates and in the solid state. Additionally, an in‐depth X‐ray diffractometric analysis supported by a Hirshfeld study of non‐covalent interactions and quantum chemical simulations was carried out. As the outcome, it was found that the content of sulfur in the compounds regulates the tuning of emission in solution as well as in the aggregated states as a consequence of their variation of planarity.
Maternal and zygotic ZFP57 regulate coronary vascular formation and myocardium maturation in mouse embryo
Authors: Zhao Junzheng , Zhao Jingjie 
Published: August 2022

AbstractBackgroundGenomic and epigenomic dynamics both play critical roles during embryogenesis. Zfp57 maintains genomic imprinting with both maternal and zygotic functions. In our previous study, we found that maternal and zygotic Zfp57 modulate NOTCH signaling during cardiac development. In this study, we investigated Zfp57 mutants from E11.5 to E13.5 to delineate its function during cardiac development.ResultsHere, we describe novel roles of maternal and zygotic Zfp57 during cardiovascular system development. We found that maternal and zygotic Zfp57 was required for coronary vascular development. Maternal and zygotic loss of Zfp57 perturbed the sprouting of the sinus venosus‐derived endothelial cells and led to underdeveloped coronary vasculature, meanwhile, there was an ectopic overproduction of blood islands over the ventricles. Furthermore, loss of Zfp57 and failed vasculature disturbed myocardium maturation. Loss of maternal and zygotic Zfp57 resulted in hyper trabeculation and failed myocardium compaction. Zfp57 zygotic mutant (M+Z−) hearts displayed noncompaction cardiomyopathy at E18.5.ConclusionsOur results suggest that maternal and zygotic ZFP57 are essential for coronary vascular formation and myocardium maturation in mice. Our research provides evidence for the role of genomic imprinting during embryogenesis.
Parsing spatial and temporal variation in stream ecosystem functioning
Authors: Mancuso Jasmine , Messick Emily , Tiegs Scott D. 
Published: August 2022

AbstractThe decomposition of organic matter is a fundamental ecosystem‐level process that governs nutrient cycling, fuels ecosystems, and impacts our global climate. Despite the central importance of this process for ecosystems across the planet, little is known about how the capacity of ecosystems to decompose organic matter—that is, their decomposition potential—varies across time and space. Closing this gap is needed to provide baseline information on the dynamics of ecosystems, for tracking anthropogenic impacts, and for development of practical and efficient sampling procedures. For nine consecutive years during the months of July–August, we deployed an identical organic‐matter decomposition assay in the same 26 temperate deciduous‐forest streams (234 stream‐years total) to evaluate the interannual and among‐stream variation in the decomposition of organic matter. Decomposition rates (as k values) ranged widely across all stream‐years (mean = 0.026, SD = 0.018, range = 0.0020–0.094). Interestingly, among‐stream variation, expressed on a per‐stream basis, was more than three times greater than interannual variation (expressed on a per year basis), explaining 75% and 22% of the overall variation in our dataset, respectively. Removing the effects of temperature by adjusting decomposition rates for degree days did not substantially impact this pattern (stream: 80% of variation, year: 17% of variation). Surprisingly, despite large differences in temperature among streams, temperature explained little variation in decomposition rates, pointing to the importance of other factors. When compared to the decomposition rates from stream sites across the planet, the rates presented here spanned 27.3% of this range, highlighting the diversity of decomposition rates that can be found in a relatively compact geographic area. From the perspective of using organic‐matter decomposition as a bioassessment tool, this variation could limit the sensitivity of decomposition‐based assays to human impacts, although study designs could be utilized to ameliorate this problem (e.g., those that stratify by geology). The results and dataset presented here are a needed step toward a more complete understanding of how ecosystem processes vary across time and space.
Two quality and stability indicating imaged CIEF methods for mRNA vaccines
Authors: Krebs Finja , Burger Udo , Dörks Susanne , Kramer Markus , Wätzig Hermann 
Published: August 2022

AbstractTwo imaged capillary isoelectric focusing methods were developed to provide insight into the quality and stability of messenger ribonucleic acid (mRNA) vaccines, specifically, mRNA encapsulated in lipid nanoparticles (LNPs). A variety of stressed and lipid composition‐modified samples were measured and detected by their UV absorption. The results were supported by the data of an encapsulation assay and particle sizing. One method, using 9 M urea as an additive, shows two broad and jagged peaks in which the peak shape offers detailed information. The summed peak area of both peaks showed RSDs from 2% to 8% when one batch was measured in triplicate and apparently depends on the size of the LNPs. In the second method, a combination of 5.5 M urea and 2 M N‐ethylurea was used. This method is characterized by a high repeatability of the isoelectric point (pI, <0.5%). The repeatable peak area (RSD of 2%–7%) correlates linearly with the mRNA content, which also applies to the first method, and added stress is evident by the change in pI and peak area. Furthermore, experiments with the addition of a fluorescent dye were performed (fluorescence detection), which tremendously increased the sensitivity of the methods. Both methods can be used to characterize the stability of mRNA‐loaded LNPs, for example, when investigating various storage times at different temperatures and freeze–thaw cycles, as well as the ability of the methods to distinguish lipid compositions and measure batch‐to‐batch variability.
Challenges for low middle‐income people with epilepsy during the COVID‐19 pandemic:Lessons learnt, call for action
Authors: Shah Urvashi , Rajeshree Shivani , Ravat Parthvi , Kalika Mayuri , Mehta Saloni , Sapre Antara , Ravat Sangeeta H. 
Published: August 2022

AbstractObjectiveThe COVID‐19 pandemic impacted the care of people with epilepsy (PWE). Several online surveys were conducted but there is limited data regarding the impact on low‐income PWE from lower‐middle income countries (LMICs) who have no access or ability to answer online surveys. The purpose of this interview was to understand the challenges faced by low‐income PWE during the lockdown phase of the pandemic.MethodPWE visiting the epilepsy specialty outpatient department of a tertiary referral government hospital to avail of subsidized services were interviewed. In the interview, they discussed challenges in obtaining medical care, the impact on wellbeing, employment, and vaccination status during the lockdown phase of the pandemic.ResultsOut of the 214 PWE interviewed, 20.6% had increased seizure frequency, 28.9% did not have access to medication mainly due to travel restrictions, 30.5% reported lack of availability of medication and 50% were not able to afford the medication mainly due to loss of income. 51% were unable to have follow‐up consultations. 36% reported worsening of mood and some reported impact on other aspects of wellbeing. The impact on wellbeing was significantly associated with an increase in seizure frequency (P < .05). The study revealed hesitation related to vaccines in the majority and expectations of financial support by the government and assistance for procuring medication. There was a lack of awareness about telemedicine services and the same was not adequately offered by government hospitals.SignificanceThe study underscores the need to learn lessons from the challenging experiences of low‐income PWE and create an action plan for the future to address the issues of lack of affordability of medical care and access to telemedicine. It is critical that the care of the marginalized, underrepresented PWE from lower‐middle income countries is not neglected during a pandemic.
A machine learning investigation into the temporal dynamics of physical activity‐mediated emotional regulation in adolescents with anorexia nervosa and healthy controls
Authors: Lekkas Damien , Gyorda Joseph A. , Jacobson Nicholas C. 
Published: August 2022

AbstractObjectiveAnorexia nervosa (AN) is commonly experienced alongside difficulties of emotion regulation (ER). Previous works identified physical activity (PA) as a mechanism for AN sufferers to achieve desired affective states, with evidence towards mitigation of negative affect. However, temporal associations of PA with specific emotional state outcomes are unknown.MethodUsing lag‐ensemble machine learning and feature importance analyses, 888 affect‐based ecological momentary assessments across N = 75 adolescents with AN (N = 44) and healthy controls (N = 31) were analysed to explore significance of past PA, measured through passively collected wrist‐worn actigraphy, with subsequent self‐report momentary affect change across 9 affect constructs.ResultsAmong AN adolescents, later lags (≥2.5 h) were important in predicting change across negative emotions (hostility, sadness, fear, guilt). AN‐specific model performance on held‐out test data revealed the holistic “negative affect” construct as significantly predictable. Only joviality and self‐assurance, both positively‐valenced constructs, were significantly predictable among healthy‐control‐specific models.DiscussionResults recapitulated previous findings regarding the importance of PA in negative ER for AN individuals. Moreover, PA was found to play a uniquely prominent role in predicting negative affect 4.5–6 h later among AN adolescents. Future research into the PA‐ER dynamic will benefit from targeting specific negative emotions across greater temporal scales.
Simulation and optimization of the waste heat recovery system of the ship power system based on the heat current method
Authors: Wei Zhi‐guo , Qiu Zhi‐qiang , Xiao Qi , Shao Wei 
Published: August 2022

AbstractWaste heat recovery technique is of great importance to improve the coefficient and reduce the emission of greenhouse gas for ship power system. This paper builds the model of a waste heat recovery system of single pressure Rankine cycle with heat current method. Combining with the thermo‐electrical analogy method, the global heat current model of waste heat recovery system is obtained, which indicates the direction of heat flow, the distribution of thermal resistance and the relationship of temperatures. Applying the law of Kirchhoff builds a mathematical model of a system with no intermediate nodes temperatures. Considering the variation of physical property and distribution of thermal resistance in phase change heat exchanger, a double nested iteration solving algorithm is proposed. The results show that the heat transfer rates of economizer, superheater evaporator, condenser, and steam receivers are 9.1, 70.42, 143.89, 19.03, 171.98, and 17.28 kW, respectively. With the objective of maximum output of turbine, applying the genetic algorithm obtains the optimal mass flow rate of cycle water, which is 0.53 kg/s and improves the output of turbine from 53.25 to 147.45 kW. Discussions on the optimization model show that decreasing extraction ratio and increasing the mass flow rates of air discharge of compressor will increase the output work of turbine. Both the optimal mass flow rate of cycle water and maximum output work decrease rapidly at first and then change little while the circulation ratio increases. Particularly, the relative variations of mass flow rate and output work are less than 2% while the circulation ratio is larger than 4.
A tug of war of forecasting the US stock market volatility: Oil futures overnight versus intraday information
Authors: Ma Feng , Wahab M. I. M. , Chevallier Julien , Li Ziyang 
Published: August 2022

AbstractThis study is the first to examine the impacts of overnight and intraday oil futures cross‐market information on predicting the US stock market volatility the high‐frequency data. In‐sample estimations present that high overnight oil futures RV can lead to high RV of the S&P 500. Moreover, negative overnight returns are more powerful than positive components, implying the existence of the leverage effect. From statistical and economic perspectives, out‐of‐sample results indicate that the decompositions of overnight oil futures and intraday RVs, based on signed intraday returns, can significantly increase the models' predictive ability. Finally, when considering the US stock market overnight effect, the decompositions are still useful to predict volatility, especially during high US stock market fluctuations and high and low EPU states.
Geochemistry, geochronology and fluid inclusion studies of the Dongji epithermal gold deposit in Fujian province, south‐east China
Authors: Huang Liang‐Liang , Fan Hong‐Rui , Ma Feng‐Shan , Liu Ri‐Fu , Wang Hui‐Sheng , Zheng Guang‐Shi , Zhang Zhi‐Cheng 
Published: August 2022

The Dongji epithermal Au deposit formed in the Early Cretaceous is located in the Dongkeng volcanic Basin of the South China Block. The orebodies of the deposit are mainly hosted in the Jurassic volcanic–subvolcanic rocks. Here, detailed zircon U–Pb geochronology, major‐trace elements, and fluid inclusion data of the Dongji deposit are reported. At least two epochs of magmatic activity were identified at Dongji, including the Late Jurassic rhyolitic crystal tuff lava (165.3 ± 1.8 Ma to 162.8 ± 2.6 Ma) and quartz porphyry (156.0 ± 1.9 Ma), and the Early Cretaceous dacitic crystal tuff lava (108.4 ± 5.7 Ma) and purplish‐red crystal tuff lava (98.1 ± 0.8 Ma). The Late Jurassic volcanic–subvolcanic rocks are characterized by high SiO2content, enriched LILE (large ion lithophile element) elements, depleted HFSE (high field strength element) elements, and obvious P, Ti and Eu anomalies. These geochemical characteristics suggest that the volcanic–subvolcanic rocks were generated by partial melting of the lower crust and controlled by fractional crystallization of Ti‐bearing phases, apatite, and plagioclase. In comparison, the Early Cretaceous volcanic–subvolcanic rocks are characterized by high K contents, and arc‐like signatures (e.g., enriched Rb, Th, K, and depleted Nb, Ta, Ti), indicating a partial melting of crust–mantle interaction under an extensional tectonic setting. The ore‐bearing dacitic crystal tuff lava and the caprock of purplish‐red tuff lava constrain the timing of gold mineralization in the range of 108–98 Ma. The Au mineralization at Dongji is divided into the early stage of quartz‐pyrite association and the late main mineralization stage of quartz‐polymetallic sulfide association. The fluid inclusion microthermometry results show that the ore‐forming fluids at the early mineralization are characterized by enrichment of liquid‐rich two‐phase inclusions and CO2‐bearing aqueous inclusions. The fluids with moderate homogenization temperatures (292–382°C) and low salinities (5.4–10.0 wt% NaClequiv) were possibly exsolved from magmatic rocks. Nevertheless, fluid inclusions in the late‐stage contain liquid‐rich two‐phase inclusions and pure liquid phase inclusions. The homogenization temperatures (222–309°C) and salinities (2.4–8.3 wt% NaClequiv) of the late‐stage fluid inclusions are relatively lower than those of the early stage, probably indicating a fluid mixing of magmatic and meteoric fluids. Based on the above, we propose that fluid mixing is the key factor facilitating Au precipitation in the Dongji deposit, probably applicable to other Au deposits in the Dongkeng volcanic Basin.
Delirium in older COVID‐19 patients: Evaluating risk factors and outcomes
Authors: Kroon Bart , Beishuizen Sara J. E. , van Rensen Inge H. T. , Barten Dennis G. , Mehagnoul‐Schipper Jannet J. , van der Bol Jessica M. , Ellerbroek Jacobien L. J. , Festen Jan , van de Glind Esther M. M. , Hempenius Liesbeth , van der Jagt Mathieu , Jansen Steffy W. M. , van der Linden Carolien J. M. , Mooijaart Simon P. , van Munster Barbara C. , Oosterwijk Leanne L. E. , Smit Lisa , Urlings‐Strop Louise C. , Willems Hanna C. , Mattace‐Raso Francesco U. S. , Polinder‐Bos Harmke A. 
Published: August 2022

AbstractObjectivesA high incidence of delirium has been reported in older patients with Coronavirus disease 2019 (COVID‐19). We aimed to identify determinants of delirium, including the Clinical Frailty Scale, in hospitalized older patients with COVID‐19. Furthermore, we aimed to study the association of delirium independent of frailty with in‐hospital outcomes in older COVID‐19 patients.MethodsThis study was performed within the framework of the multi‐center COVID‐OLD cohort study and included patients aged ≥60 years who were admitted to the general ward because of COVID‐19 in the Netherlands between February and May 2020. Data were collected on demographics, co‐morbidity, disease severity, and geriatric parameters. Prevalence of delirium during hospital admission was recorded based on delirium screening using the Delirium Observation Screening Scale (DOSS) which was scored three times daily. A DOSS score ≥3 was followed by a delirium assessment by the ward physician In‐hospital outcomes included length of stay, discharge destination, and mortality.ResultsA total of 412 patients were included (median age 76, 58% male). Delirium was present in 82 patients. In multivariable analysis, previous episode of delirium (Odds ratio [OR] 8.9 [95% CI 2.3–33.6] p = 0.001), and pre‐existent memory problems (OR 7.6 [95% CI 3.1–22.5] p < 0.001) were associated with increased delirium risk. Clinical Frailty Scale was associated with increased delirium risk (OR 1.63 [95%CI 1.40–1.90] p < 0.001) in univariable analysis, but not in multivariable analysis. Patients who developed delirium had a shorter symptom duration and lower levels of C‐reactive protein upon presentation, whereas vital parameters did not differ. Patients who developed a delirium had a longer hospital stay and were more often discharged to a nursing home. Delirium was associated with mortality (OR 2.84 [95% CI1.71–4.72] p < 0.001), but not in multivariable analyses.ConclusionsA previous delirium and pre‐existent memory problems were associated with delirium risk in COVID‐19. Delirium was not an independent predictor of mortality after adjustment for frailty.