bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a quantitative methylation-specific droplet digital PCR assay for detecting Dickkopf-related protein 3
Authors: Araki Kenji , Kurosawa Ai , Kumon Hiromi 
Published: May 2022

Abstract
Objective
The detection and monitoring of DNA methylation status in circulating tumor cell DNA (ctDNA) provides critical insights into cancer diagnosis and progression. The methylation status of the Dickkopf-related protein 3 (DKK3) promoter region is correlated with the metastasis and recurrence of multiple cancers. Thus, detecting the methylation status via non-invasive methods is essential for the diagnosis and prognosis of cancers. Using a droplet digital polymerase chain reaction approach, we have developed a highly sensitive and quantitative measurement of methylated and unmethylated DKK3 derived from circulating cell-free DNA (ccfDNA).

Results
We confirmed the specificity of droplet digital methylation specific polymerase chain reaction (ddMSP). We selected the optimal bisulfite conversion method using commercially available kits. We validated the ddMSP analysis system by analyzing the methylation status of genomic DNA extracted from cultured mesothelioma cells and mesothelial cells. Our system quantified approximately 30 copies of cell-free DNA per 4 mL, which is sufficient for detecting ctDNA. Finally, we quantified methylated and unmethylated DKK3 copies in ccfDNA from 21 patients with malignant mesothelioma.
Elucidating the role of the gut microbiota in the physiological effects of dietary fiber
Authors: Deehan Edward C. , Zhang Zhengxiao , Riva Alessandra , Armet Anissa M. , Perez-Muñoz Maria Elisa , Nguyen Nguyen K. , Krysa Jacqueline A. , Seethaler Benjamin , Zhao Yuan-Yuan , Cole Janis , Li Fuyong , Hausmann Bela , Spittler Andreas , Nazare Julie-Anne , Delzenne Nathalie M. , Curtis Jonathan M. , Wismer Wendy V. , Proctor Spencer D. , Bakal Jeffrey A. , Bischoff Stephan C. , Knights Dan , Field Catherine J. , Berry David , Prado Carla M. , Walter Jens 
Published: May 2022

AbstractBackgroundDietary fiber is an integral part of a healthy diet, but questions remain about the mechanisms that underlie effects and the causal contributions of the gut microbiota. Here, we performed a 6-week exploratory trial in adults with excess weight (BMI: 25–35 kg/m2) to compare the effects of a high-dose (females: 25 g/day; males: 35 g/day) supplement of fermentable corn bran arabinoxylan (AX;n= 15) with that of microbiota-non-accessible microcrystalline cellulose (MCC;n= 16). Obesity-related surrogate endpoints and biomarkers of host-microbiome interactions implicated in the pathophysiology of obesity (trimethylamineN-oxide, gut hormones, cytokines, and measures of intestinal barrier integrity) were assessed. We then determined whether clinical outcomes could be predicted by fecal microbiota features or mechanistic biomarkers.ResultsAX enhanced satiety after a meal and decreased homeostatic model assessment of insulin resistance (HOMA-IR), while MCC reduced tumor necrosis factor-α and fecal calprotectin. Machine learning models determined that effects on satiety could be predicted by fecal bacterial taxa that utilized AX, as identified by bioorthogonal non-canonical amino acid tagging. Reductions in HOMA-IR and calprotectin were associated with shifts in fecal bile acids, but correlations were negative, suggesting that the benefits of fiber may not be mediated by their effects on bile acid pools. Biomarkers of host-microbiome interactions often linked to bacterial metabolites derived from fiber fermentation (short-chain fatty acids) were not affected by AX supplementation when compared to non-accessible MCC.ConclusionThis study demonstrates the efficacy of purified dietary fibers when used as supplements and suggests that satietogenic effects of AX may be linked to bacterial taxa that ferment the fiber or utilize breakdown products. Other effects are likely microbiome independent. The findings provide a basis for fiber-type specific therapeutic applications and their personalization.Trial registrationClinicaltrials.gov,NCT02322112, registered on July 3, 2015.
The effectiveness of adapted group mindfulness-based stress management program on perceived stress and emotion regulation in midwives: a randomized clinical trial
Authors: Aghamohammadi Fatemeh , Saed Omid , Ahmadi Reza , Kharaghani Roghieh 
Published: May 2022

AbstractBackgroundMidwives' stress can have negative consequences on their emotional state, burnout, and poor quality of midwifery care. This study aimed to determine the effectiveness of an adapted mindfulness-based stress management program on perceived stress and the emotional regulation of midwives.MethodsThe study was a parallel randomized clinical trial on the midwives working in general hospitals of Zanjan, Iran. In this study, 121 midwives registered to participate based on the census sampling method were screened using a cut point of ≥ 28 in the Perceived Stress Scale (PSS). From the initial sample, 42 subjects had inclusion criteria assigned to two groups of control (n = 21) and intervention (n = 21) using online random allocation. The intervention group received an 8-week adapted mindfulness-based stress management program. This program emanates from the Kabat-Zinn's MBSR program, which has been adjusted according to the Iranian culture. The ANCOVA and repeated measure analysis of variance test were used to compare groups over time.ResultsThe results showed that the group intervention effectively affected perceived stress (P = 0.001) and difficulty in emotion regulation during the post-intervention period (P = 0.001). Moreover, the interventions were effective in emotion regulation (P = 0.003), but it was not effective on perceived stress (P = 0.125) at the 3-month follow-up.ConclusionsThis adapted mindfulness-based program successfully reduced stress and increased emotion regulation strategies in midwives; however, the long-term outcomes of this treatment program need further consideration.
Atomevo: a web server combining protein modelling, docking, molecular dynamic simulation and MMPBSA analysis of Candida antarctica lipase B (CalB) fusion protein
Authors: Hao Jin-Heng , Zheng Dun-Jin , Ye Yu-Hao , Yu Jie-Ting , Li Xin-Yao , Xiong Mei-Jie , Jiang Wen-Hao , He Kang-Ping , Li Pei-Yu , Lv Yong-Si , Gu Wei-Ming , Lai Lin-Hao , Wu Yi-Da , Cao Shi-Lin 
Published: May 2022

AbstractAlthough current computational biology software is available and has prompted the development of enzyme–substrates simulation, they are difficult to install and inconvenient to use. This makes the time-consuming and error-prone process. By far there is still a lack of a complete tool which can provide a one-stop service for the enzyme–substrates simulation process. Hence, in this study, several computational biology software was extended development and integrated as a website toolbox named Atomevo. The Atomevo is a free web server providing a user-friendly interface for enzyme–substrates simulation: (1) protein homologous modeling; (2) parallel docking module of Autodock Vina 1.2; (3) automatic modeling builder for Gromacs molecular dynamics simulation package; and (4) Molecular Mechanics/Poisson–Boltzmann Surface Area (MMPBSA) analysis module for receptor–ligand binding affinity analysis. We officially launched the web server and provided instructions through a case for the design and simulation of Candida antarctica lipase B (CalB) fusion protein called Maltose Binding Protein—Thioredoxin A—Candida antarctica lipase B (MBP-TrxA-CalB).
Graphical Abstract
Match Analysis in Team Ball Sports: An Umbrella Review of Systematic Reviews and Meta-Analyses
Authors: Sarmento Hugo , Clemente Filipe Manuel , Afonso José , Araújo Duarte , Fachada Miguel , Nobre Paulo , Davids Keith 
Published: May 2022

AbstractBackgroundMatch analysis has evolved exponentially over the past decades in team sports resulting in a significant number of published systematic reviews and meta-analyses. An umbrella review of the available literature is needed to provide an integrated overview of current knowledge and contribute to more robust theoretical explanations of team performance.MethodsThe Web of Science (all databases), PubMed, Cochrane Library (Cochrane Database of Systematic Reviews), Scopus, and SPORTDiscus databases were searched for relevant publications prior to 19 February 2021. Appraisal of the methodological quality of included articles was undertaken using the tool for Assessing the Methodological Quality of Systematic Reviews (AMSTAR-2). Twenty-four studies were reviewed that met the following criteria: (1) contained relevant data from match analyses in team ball sports; (2) were defined as systematic reviews or/and meta-analyses; and (3) were written in the English language.ResultsThe overall methodological quality of the 24 included reviews, obtained through the AMSTAR-2, revealed very low confidence ratings (Critically Low,n = 12) for the results of most systematic reviews of match analyses in team ball sports. Additionally, the results showed that research is focused mainly on four levels of analysis: (1) dyadic (microlevel); (2) individual (molecular level; predominant); (3) group (mesolevel), and (4) team dynamics (macrolevel). These levels of analysis included tactical, technical, physical, and psychosocial variables. Team performance was contextualized at two levels, with reference to: (1) match context (e.g. match status, match location, match period, quality of opposition) and (2) sociodemographic and environmental constraints (sex, age groups, competitive level, altitude, temperature, pitch surface).ConclusionsThe evolution of methods for match analysis in team ball sports indicates that: (1) an individual-level performance analysis was predominant; (2) the focus on intermediate levels of analysis, observing performance in dyadic and group interactions, has received less attention from researchers; (3) neglected areas of research include psychosocial aspects of team sports and women’s performance; and (4) analyses of match contexts need greater depth.Registration: The protocol was registered in the International Platform of Registered Systematic Review and Meta-Analysis Protocols with the number 202080067 and the DOI numberhttps://doi.org/10.37766/inplasy2020.8.0067.
Extracorporeal Shockwave Therapy for Mid-portion and Insertional Achilles Tendinopathy: A Systematic Review of Randomized Controlled Trials
Authors: Paantjens Marc A. , Helmhout Pieter H. , Backx Frank J. G. , van Etten-Jamaludin Faridi S. , Bakker Eric W. P. 
Published: May 2022

Abstract
Background
Extracorporeal shockwave therapy (ESWT) is used commonly to treat pain and function in Achilles tendinopathy (AT). The aim of this study was to synthesize the evidence from (non-) randomized controlled trials, to determine the clinical effectiveness of ESWT for mid-portion Achilles tendinopathy (mid-AT) and insertional Achilles tendinopathy (ins-AT) separately.

Methods
We searched PubMed/Medline, Embase (Ovid), and Cochrane Central, up to January 2021. Unpublished studies and gray literature were searched in trial registers (ACTRN, ChiCTR, ChiCtr, CTRI, DRKS, EUCTR, IRCT, ISRCTN, JPRN UMIN, ClinicalTrials.gov, NTR, TCTR) and databases (OpenGrey.eu, NARCIS.nl, DART-Europe.org, OATD.org). Randomized controlled trials (RCTs) and non-randomized controlled clinical trials (CCTs) were eligible when investigating the clinical effectiveness of ESWT for chronic mid-AT or chronic ins-AT. We excluded studies that focused on treating individuals with systemic conditions, and studies investigating mixed cohorts of mid-AT and ins-AT, when it was not possible to perform a subgroup analysis for both clinical entities separately. Two reviewers independently performed the study selection, quality assessment, data extraction, and grading of the evidence levels. Discrepancies were resolved through discussion or by consulting a third reviewer when necessary.

Results
We included three RCTs on mid-AT and four RCTs on ins-AT. For mid-AT, moderate quality of evidence was found for the overall effectiveness of ESWT compared to standard care, with a pooled mean difference (MD) on the VISA-A of 9.08 points (95% CI 6.35–11.81). Subgroup analysis on the effects of ESWT additional to standard care for mid-AT resulted in a pooled MD on the VISA-A of 10.28 points (95% CI 7.43–13.12). For ins-AT, we found very low quality of evidence, indicating that, overall, ESWT has no additional value over standard care, with a standardized mean difference (SMD) of − 0.02 (95% CI − 0.27 to 0.23). Subgroup analysis to determine the effect of ESWT additional to standard care for ins-AT showed a negative effect (SMD − 0.29; 95% CI − 0.56 to − 0.01) compared to standard care alone.

Conclusions
There is moderate evidence supporting the effectiveness of ESWT additional to a tendon loading program in mid-AT. Evidence supporting the effectiveness of ESWT for ins-AT is lacking.
Trial Registration: PROSPERO Database; No. CRD42021236107.
Isolation, identification and efficacy of three bacterial isolates against the red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)
Authors: Almasoudi Najeeb M. , Asiry Khalid A. , Abo-Elyousr Kamal A. M. 
Published: May 2022

Abstract
Background
The red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), overruns date palm ranches in most Oases of Saudi Arabia and causes massive economic losses. Integrated pest management (IPM) of the RPW by biocontrol agents of bacterial isolates was the primary aim of the present study.

Results
Thirty-seven bacterial isolates were isolated from the larvae of R. ferrugineus, which were collected from infested Phoenix dactylifera trees growing in different parts of Saudi Arabia. 16S rDNA sequencing showed that the isolated bacteria were: Serratia marcescens, Klebsiella pneumoniae and Bacillus thuringiensis. The potential of these isolates was tested against R. ferrugineus in the laboratory. The use of these three isolates showed significant mortality percentages against R. ferrugineus. The highest reduction in mortality was recorded at the concentration of 1 × 108 CFU/ml; B. thuringiensis isolate displayed the highest potency in mortal percentage rate (100%), 4 days post-treatment, followed by S. marcescens isolate after 5 days, and then K. pneumoniae isolate after 6 days.

Conclusions
These results suggest that the use of these bacterial isolates was very useful in in vitro experiments, and it may consider those bacterial strains of interest as a potential biocontrol agent of R. ferrugineus.
Cavernous haemangioma of cavernous sinus causing diagnostic dilemma: a case report with review of literature
Authors: Mishra Sarvesh Chandra , Chaturvedi Pragya , Gupta Aviral , Sharma Srishti 
Published: May 2022

Abstract
Background
Cavernous haemangioma of the cavernous sinus is an uncommon vascular malformation. It is often confused with other parasellar masses like schwannomas and meningiomas due to overlap in imaging appearance. It is important to pre-operatively diagnose this condition as it is associated with severe intra-operative bleeding.

Case presentation
Here, we report a case of an octogenarian female who presented with left sided progressive ptosis and diminution of vision for one year. Cross-sectional imaging includes computed tomography and magnetic resonance imaging which were not conclusive of cavernous sinus haemangioma. Digital subtraction angiography was done which showed a vascular blush. After corroborating the findings of these imaging investigations, a diagnosis of cavernous sinus haemangioma was concluded. Patient underwent surgery which showed a hypervascular mass. Histopathology was consistent with the imaging diagnosis. Patient was discharged in good stable condition and doing well and is on follow-up in Neurosurgery Out-Patient Department.

Conclusions
The cavernous sinus Haemangiomas are uncommon benign vascular masses. They pose a diagnostic challenge when seen in the parasellar region as the imaging findings of a haemangioma, meningioma and schwannoma in this location can have a significant overlap in the imaging findings. Cross-sectional imaging, digital subtraction angiography and nuclear imaging help in pre-operative diagnosis which is a crucial as the surgery is associated with significant blood loss.
Multimodality radionuclide imaging in fever of unknown origin presenting with a solitary spleen lesion
Authors: Filippi Luca , Bagni Oreste , Schillaci Orazio 
Published: May 2022

Abstract
Background
Fever of unknown origin (FUO) still represents a serious challenge for clinicians, since it can be related to a wide spectrum of disorders, ranging from infections to malignancies. In this scenario, nuclear medicine can be of value to achieve a correct diagnosis both through positron emission computed tomography (PET/CT) and 99mTc labeled hexamethylpropylene amine oxime (HMPAO) white blood cell (WBC) scintigraphy.

Case presentation
We are presenting the case of 65-year-old male, who was referred to our hospital due to prolonged unexplained fever. He was submitted to abdomen ultrasonography (US) that did not disclose relevant pathological findings. Subsequently, he underwent PET/CT scan with 18F-fluorodeoxyglucose (18F-FDG) that revealed an area of increased tracer uptake in splenic inferior pole. In order to solve differential diagnosis between tumor and infection, he was submitted to 99mTc-HMPAO WBC scintigraphy that resulted negative for sites of pathologic radiolabeled cells’ accumulation but revealed a photopenic area in the splenic inferior pole. The pattern of mismatched uptake between 18F-FDG PET/CT and 99mTc-HMPAO WBC scintigraphy was considered highly suspicious for spleen tumor localization. The patient was scheduled for splenectomy and histology resulted positive for non-Hodgkin lymphoma (NHL) of diffuse large B cell type. After splenectomy, a further 18F-FDG PET/CT revealed the appearance of hypermetabolic hepatic lesions. The patient underwent chemotherapy with complete remission.

Conclusion
Nuclear medicine provides valuable tools for differential diagnosis in FUO. In case of patients presenting solitary lesion of the spleen, the combined use of 18F-FDG PET/CT and 99mTc-HMPAO WBC scintigraphy can provide relevant information to aid clinicians to a correct diagnosis.
Ironic Disagreement and Objection in Everyday Communication
Authors: Luzhnaya M. 
Published: May 2022

The paper describes the speech acts of disagreement and objection. An attempt to differentiate these speech acts is made. Direct and conventional indirect speech acts that implement these intentions are presented in dictionaries and textbooks on speech etiquette, as well as described in the scientific literature. The systematic research of contextual and situational indirect speech acts of disagreement and objection has not been practically carried out.
The research focuses on ironic contextual and situational indirect speech acts of disagreement and objection ("the second degree of indirection"). The aim of the paper is to describe the functioning of these speech acts.
The methods of definitional analysis, discursive analysis, quantitative analysis, as well as descriptive and analytical method were used in the research.
The analysis of the material revealed that ironic contextual and situational indirect speech acts of disagreement and objection have the forms of representatives, rogatives, directives, expressives. The research considers examples of combining the intentions of disagreement and objection with other intentions (advice and reproach) in polyintentional contextual and situational indirect speech acts.
The scientific novelty of the study lies in the fact that it analyzes the ironic contextual and situational indirect speech acts of disagreement and objections in everyday communication. The analysis of indirect speech implementations of these intentions is carried out.
Using the results of the research can be useful for further study of everyday communication and in practical activities of teachers who are taking up the problems of communication and language teaching.
Indonesian nursing students' intention to accept COVID-19 vaccines: an online, multicentre survey
Authors: Sirait Healthy Seventina , Saidah Qori'ila , Hasanah Oswati , Hanifah Astin Nur , Arifin Hidayat , Rosyad Yafi Sabila , Rias Yohanes Andy 
Published: May 2022

Background: The determinants of intention to accept the COVID-19 vaccine and associated factors among nursing students during this pandemic need to be identified. Aims: The study assessed nursing students' attitudes to vaccination, health engagement and trust in government, as well as their intention to have a COVID-19 vaccination. Methods: A cross-sectional study was carried out among nursing students in three provinces of Java, Indonesia, from December 2020 to February 2021. Convenience sampling was used to gather primary data from 640 participants through a structured online survey. Multiple linear regression was used to analyse the results. Findings: All items of vaccine attitude and health engagement as well as trust in government were positive predictors of vaccine acceptance after adjusting for confounding factors. Conclusion: Nursing students were more likely to accept vaccination if they had a positive attitude to vaccination, high levels of health engagement and trust in the government regarding tackling the COVID-19 pandemic.
Temporomandibular disorders. Part 2: non-surgical management
Authors: Foster-Thomas Emma , James Martin , Crawford Charles , Clarke Pete , Oluwajana Funmi , Lancelott-Redfern Cathleen 
Published: May 2022

For optimal success, a multidisciplinary team approach that uses a combination of non-surgical treatment modalities is recommended in the care of TMD patients. In this article, the second in a series of six focusing on the diagnosis and management of temporomandibular disorders (TMD), the importance of self-management, psychological interventions and physical therapies is discussed. Intra-oral appliances will be covered in a dedicated article later in this series. CPD/Clinical Relevance: Clinicians should have both an understanding and appreciation for the non-surgical management options available to patients diagnosed with TMD.
The role of AGEs in pathogenesis of cartilage destruction in osteoarthritis
Authors: He Chao-Peng , Chen Cheng , Jiang Xin-Chen , Li Hui , Zhu Li-Xin , Wang Ping-Xiao , Xiao Tao 
Published: May 2022

Osteoarthritis (OA) is a degenerative disease resulting from progressive joint destruction caused by many factors. Its pathogenesis is complex and has not been elucidated to date. Advanced glycation end products (AGEs) are a series of irreversible and stable macromolecular complexes formed by reducing sugar with protein, lipid, and nucleic acid through a non-enzymatic glycosylation reaction (Maillard reaction). They are an important indicator of the degree of ageing. Currently, it is considered that AGEs accumulation in vivo is a molecular basis of age-induced OA, and AGEs production and accumulation in vivo is one of the important reasons for the induction and acceleration of the pathological changes of OA. In recent years, it has been found that AGEs are involved in a variety of pathological processes of OA, including extracellular matrix degradation, chondrocyte apoptosis, and autophagy. Clearly, AGEs play an important role in regulating the expression of OA-related genes and maintaining the chondrocyte phenotype and the stability of the intra-articular environment. This article reviews the latest research results of AGEs in a variety of pathological processes of OA, to provide a new direction for the study of OA pathogenesis and a new target for prevention and treatment. Cite this article: Bone Joint Res 2022;11(5):292–300.