bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simple and practical sialoglycan encoding system reveals vast diversity in nature and identifies a universal sialoglycan-recognizing probe derived from AB5 toxin B subunits
Authors: Sasmal Aniruddha , Khan Naazneen , Khedri Zahra , Kellman Benjamin P , Srivastava Saurabh , Verhagen Andrea , Yu Hai , Bruntse Anders Bech , Diaz Sandra , Varki Nissi , Beddoe Travis , Paton Adrienne W , Paton James C , Chen Xi , Lewis Nathan E , Varki Ajit 
Published: September 2022

Abstract
Vertebrate sialic acids (Sias) display much diversity in modifications, linkages, and underlying glycans. Slide microarrays allow high-throughput explorations of sialoglycan–protein interactions. A microarray presenting ~150 structurally defined sialyltrisaccharides with various Sias linkages and modifications still poses challenges in planning, data sorting, visualization, and analysis. To address these issues, we devised a simple 9-digit code for sialyltrisaccharides with terminal Sias and underlying two monosaccharides assigned from the nonreducing end, with 3 digits assigning a monosaccharide, its modifications, and linkage. Calculations based on the encoding system reveal &gt;113,000 likely linear sialyltrisaccharides in nature. Notably, a biantennary N-glycan with 2 terminal sialyltrisaccharides could thus have &gt;1010 potential combinations and a triantennary N-glycan with 3 terminal sequences, &gt;1015 potential combinations. While all possibilities likely do not exist in nature, sialoglycans encode enormous diversity. While glycomic approaches are used to probe such diverse sialomes, naturally occurring bacterial AB5 toxin B subunits are simpler tools to track the dynamic sialome in biological systems. Sialoglycan microarray was utilized to compare sialoglycan-recognizing bacterial toxin B subunits. Unlike the poor correlation between B subunits and species phylogeny, there is stronger correlation with Sia-epitope preferences. Further supporting this pattern, we report a B subunit (YenB) from Yersinia enterocolitica (broad host range) recognizing almost all sialoglycans in the microarray, including 4-O-acetylated-Sias not recognized by a Yersinia pestis orthologue (YpeB). Differential Sia-binding patterns were also observed with phylogenetically related B subunits from Escherichia coli (SubB), Salmonella Typhi (PltB), Salmonella Typhimurium (ArtB), extra-intestinal E.coli (EcPltB), Vibrio cholera (CtxB), and cholera family homologue of E. coli (EcxB).
Feasibility of a remotely delivered program to prevent Latino adolescent obesity in the Midwestern USA
Authors: Nagao-Sato Sayaka , de Davila Silvia Alvarez , Monardez Popelka Javiera , Burk Gabriela , Soltani Darya , Baltaci Aysegul , Peralta Reyes Alejandro Omar , Rodriguez Matt , Reicks Marla , Hurtado Choque Ghaffar Ali 
Published: September 2022

Summary
Adolescent obesity prevention programs focusing on Latino fathers may be useful to address Latino adolescent obesity. Adolescent obesity has become an urgent issue because of the coronavirus disease 2019 pandemic, with limited ability to deliver prevention programs in-person. This study aimed to assess the feasibility of a community-based, adolescent obesity prevention program for Latino father–adolescent dyads delivered remotely, adapted from the in-person Padres Preparados, Jóvenes Saludables program. A quasi-experimental design was used to assess the feasibility of the remotely delivered program based on criteria adapted from other feasibility studies of community-based health promotion programs for Latino families. Father–adolescent dyads were recruited at two sites in a Midwestern state during 2020; mothers were also encouraged to participate. Recruitment met the feasibility criteria (65 families expressed interest between August and October) with 26 families participating in this study. The retention ratio (percentage of participants who completed a post-session survey to those who completed the baseline survey) among fathers was acceptable (77%), while a lower percentage of adolescents were retained (68%). The session attendance ratio (number of sessions attended of the eight total sessions offered) was higher among adolescents (88%) and lower among fathers (68%) compared to the criteria. Satisfaction ratings (≥88%) and completeness of data collection at both baseline and post-session survey (≥76%) were acceptable. Favorable results were obtained for parent outcomes, while adolescent outcomes were not favorably changed. This remotely delivered adolescent obesity prevention program was feasible for Latino fathers; however, additional engagement with adolescents may be needed.
Phenology and environmental drivers of glass eel entrance in a Mediterranean lagoon
Authors: Lagarde Raphaël , Peyre Jason , Amilhat Elsa , Bourrin François , Prellwitz François , Perrault Alexandre , Simon Gaël , Faliex Elisabeth 
Published: September 2022

AbstractThe European eel, Anguilla anguilla, is an emblematic facultative catadromous species that spawns in the Sargasso Sea and grow in continental waters of Europe and North Africa. In most of its growing habitats its population has dropped since 1980. Although Mediterranean lagoons represent particularly important habitats for eel, knowledge of eel ecology in lagoons is not as developed as it is in rivers. Particularly, data on the phenology, drivers and biometrics characteristics of glass eel entering lagoons are scarce. To address this lack of data, the abundance, pigmentation stage, size, and weight of glass eel entering the Bages-Sigean lagoon (western French Mediterranean) were monitored during 647 d from December 2018 to April 2021 using passive floating traps. Simultaneously, different environmental drivers were measured (flow velocity, temperature, rainfalls…). The highest abundances of glass eels were observed between mid-November and mid-March especially when the discharge of the main tributary of the lagoon was higher than its base flow. The glass eels captured during the peak of entrance were less pigmented, longer, and bigger than during other months. This work enabled us to identify periods when anthropogenic activities should be limited to decrease human-based impact on glass eel in similar habitats.
A retrospective study of drugs associated with xerostomia from the Australian Database of Adverse Event Notifications
Authors: Choo Pei Jin , Taing Meng-Wong , Teoh Leanne 
Published: September 2022

AbstractObjectivesXerostomia is a subjective sensation of dry mouth associated with many medications and increases the risk of tooth decay and other oral complications. The aim of this study was to identify unreported medications associated with dry mouth from the Australian Database of Adverse Event Notifications (ADAEN) from the Therapeutic Goods Administration (TGA) in Australia.MethodsThis was a descriptive retrospective study. A request was made to the TGA to provide all reports associated with dry mouth. De-identified reports were provided from the commencement of the database in 1971 until June 2020. Drugs were divided into established drugs that are associated with xerostomia in the primary literature and secondary drugs not reported in the primary literature.Key findingsThere were 1927 individual case reports for dry mouth associated with medications. Of these, there were 1379 reports of established (primary) drugs and 1481 reports of secondary drugs associated with xerostomia. Dry mouth was found to be associated with many medication classes; analgesics, cardiovascular and gastrointestinal drugs had the greatest number of secondary drugs reported.ConclusionsA comprehensive list of suspected medications associated with xerostomia has been established. This adds to the growing catalogue of medications associated with dry mouth, where several medications have not previously been identified in the primary literature.
Phenotypic and genotypic antimicrobial susceptibility patterns of the emerging human respiratory pathogen Mycoplasma amphoriforme isolated from the UK and Denmark
Authors: Day Jessica , Afshar Baharak , Rowlands Richard S , Umer Taiba S , Windsor Helena , Paukner Susanne , Jensen Jorgen S , Spiller Owen B , Chalker Victoria J , Beeton Michael L ,   the ESCMID Study Group for Mycoplasma and Chlamydia Infections (ESGMAC), Beeton Michael , Jensen Jorgen Skov , Mestrovic Tomislav , Pereyre Sabine , Van Der Pol Barbara 
Published: September 2022

Abstract

Objectives
To determine the phenotypic and genotypic antibiotic susceptibility of Mycoplasma amphoriforme isolates recovered from patients in the UK and Denmark.

Methods
Seven isolates of M. amphoriforme were examined for antimicrobial susceptibility to seven antibiotics using the microbroth dilution assay in line with the CLSI guidelines for mycoplasmas. Each isolate was additionally subjected to WGS to identify resistance-associated mutations. Based on the consensus sequences from the genomic data, PCR primers were designed, and tested, for the amplification of the QRDR within the parC gene.

Results
Of the seven isolates investigated, four (57%) were resistant to moxifloxacin (0.5–1 mg/L) and levofloxacin (1–2 mg/L), compared with those that were susceptible (0.03–0.06 and 0.006 mg/L, respectively). Isolate H29 was resistant to five of the seven antibiotics tested: moxifloxacin, 0.5 mg/L; levofloxacin, 2 mg/L; azithromycin, 64 mg/L; erythromycin, 128 mg/L; and clindamycin, 64 mg/L. All isolates were susceptible to tetracycline (0.06 mg/L) and lefamulin (0.001–0.004 mg/L). Mutations from genomic data confirmed the presence of an S89F mutation within the ParC protein among all fluoroquinolone-resistant isolates and an A2059G mutation in the 23S rRNA gene in the macrolide- and lincosamide-resistant isolate H29.

Conclusions
To the best of our knowledge, this is the first time where phenotypic and genotypic resistance data have been paired for M. amphoriforme confirming a correlation between the two. These data suggest the need for focused testing and resistance determination of isolates from high-risk patients given the backdrop of a high prevalence of antimicrobial resistance.
Model to Inhibit Contraction in Third-Degree Burns Employing Split-Thickness Skin Graft and Administered Bone Marrow-Derived Stem Cells
Authors: Rodriguez-Menocal Luis , Davis Stephen C , Guzman Wellington , Gil Joel , Valdes Jose , Solis Michael , Higa Alexander , Natesan Shanmugasundaram , Schulman Carl I , Christy Robert J , Badiavas Evangelos V 
Published: September 2022

Abstract
Third-degree burns typically result in pronounced scarring and contraction in superficial and deep tissues. Established techniques such as debridement and grafting provide benefit in the acute phase of burn therapy, nevertheless, scar and contraction remain a challenge in deep burns management. Our ambition is to evaluate the effectiveness of novel cell-based therapies, which can be implemented into the standard of care debridement and grafting procedures. Twenty-seven third-degree burn wounds were created on the dorsal area of Red Duroc pig. After 72 h, burns are surgically debrided using a Weck knife. Split-thickness skin grafts (STSGs) were then taken after debridement and placed on burn scars combined with bone marrow stem cells (BM-MSCs). Biopsy samples were taken on days 17, 21, and 45 posttreatment for evaluation. Histological analysis revealed that untreated control scars at 17 days are more raised than burns treated with STSGs alone and/or STSGs with BM-MSCs. Wounds treated with skin grafts plus BM-MSCs appeared thinner and longer, indicative of reduced contraction. qPCR revealed some elevation of α-SMA expression at day 21 and Collagen Iα2 in cells derived from wounds treated with skin grafts alone compared to wounds treated with STSGs + BM-MSCs. We observed a reduction level of TGFβ-1 expression at days 17, 21, and 45 in cells derived from wounds treated compared to controls. These results, where the combined use of stem cells and skin grafts stimulate healing and reduce contraction following third-degree burn injury, have a potential as a novel therapy in the clinic.
Chlordimeform: A Detachment-Stimulating Chemical for Three-Host Ticks
Authors: Gladney W. J. , Ernst S. E. , Drummond R. O. 
Published: September 2022

Abstract
Nymphs and adults of Amblyomma americanum (L.), Rhipicephalus sanguineus (Latreille), and Dermacentor andersoni Stiles attached Co guinea pigs were topically treated with 0.03125-1.0% chlordimeform [N'-(4-chloro-o-tolyl)-N,N-dimethylformamidine] in 1 ul of acetone. At 0.5-1.0%, 83.9-85.5% of adult A. americanum and at 0.125-0.25%, all adult R. sanguineus and D. andersoni detached within 2 hr of treatment. Chlord meform was less effective as a tick detachment agent with nymphs than adults but was more toxic to nymphs than adults. The compound was highly effective in causing ticks to detach; it alto caused a slow and incomplete kill of the detached ticks.
A guide to hydrogen legislation in the USA: a renewed effort
Authors: Talus Kim , Martin Maxwell 
Published: September 2022

Abstract
This article will discuss how hydrogen-based energy could resolve green-energy uptake issues in the USA. In doing so, this article will first present the unique characteristics of hydrogen and its production methods. Next, this article will detail prior US hydrogen policy which often focused on research and development (R&D) rather than implementation strategies. Later, this paper will delve into the Biden Administration’s recent legislation Infrastructure and Jobs Act (IJA) and the Inflation Reduction Act (IRA) and the hydrogen production incentivization policies enacted. And last, this article will delve into issues that were not resolved by the recent legislation.
Detection of circulating DNA for the diagnosis of invasive fusariosis: retrospective analysis of 15 proven cases
Authors: Dellière Sarah , Guitard Juliette , Sabou Marcela , Angebault Cécile , Moniot Maxime , Cornu Marjorie , Hamane Samia , Bougnoux Marie-Elisabeth , Imbert Sébastien , Pasquier Grégoire , Botterel Françoise , Garcia-Hermoso Dea , Alanio Alexandre 
Published: September 2022

AbstractFusarium spp. are plant pathogens and opportunistic pathogens in severely immunocompromised (hematological malignancy, neutropenia, solid organ transplantation, etc.) and severely burned patients. Invasive fusariosis often disseminates and mortality remains high partly due to delayed diagnosis in the absence of a positive culture. The aim of our study is to design a quantitative PCR (qPCR) assay and evaluate the detection of Fusarium spp. DNA for early diagnosis of invasive infection. A qPCR assay was designed and optimized to identify all Fusarium species complex and secondarily evaluated on patient samples. A total of 81 blood samples from 15 patients diagnosed with proven invasive fusariosis from 9 centers in France were retrospectively tested. Circulating DNA was detected in 14 patients out of 15 (sensitivity of 93% [95% Confidence Interval (CI95), 70.1-99.7]). Detection was possible up to 18 days (median 6 days) before the diagnosis was confirmed by positive blood culture or biopsy. By comparison serum galactomannan and ß-D-glucan were positive in 7.1 and 58.3% of patients respectively. qPCR was negative for all patients with other invasive fungal diseases (IFD) tested (n = 12) and IFD-free control patients (n = 40). No cross-reactions were detected using DNA extracted from 81 other opportunistic fungi. We developed and validated a pan-Fusarium qPCR assay in serum/plasma with high sensitivity, specificity, and reproducibility that could facilitate early diagnosis and treatment monitoring of invasive fusariosis.Lay AbstractFusariosis ranks third among invasive mould infections. It is frequently diagnosed late due to the lack of specific tools. We designed and evaluated a new qPCR assay with high sensitivity and specificity allowing detection of Fusarium DNA in serum samples up to 18 days before conventional diagnosis.
Conclusion
Authors: Baumgartner Michael 
Published: September 2022

Abstract
The first part of this concluding chapter summarizes Godard’s concept of film music. He self-consciously approaches film music by either fragmenting musical compositions or repeating the unaltered fragments several times in the same film. Fragmentation and repetition are two devices with which Godard questions the validity of the leitmotif technique for cinematic use. He further explores music with quotations by self-consciously integrating into his films works of the Western musical canon or French, English, American, and Italian popular songs. He also expands the concept of quotation to referencing self-reflexively genre-specific film music. Critically questioning the function of film music in musical comedies, films noir, science-fiction films, and melodramas, he pastiches or parodies music associated with these stereotypical Hollywood genres in several of his 1960s films. A last category of metafilm music in Godard’s work is concerned with music making as a metaphor for filmmaking. This analogy between the two arts was prompted by the increasing intensification in the 1980s of his creative crisis as a postmodern filmmaker struggling with completing a meaningful work. The second part of this concluding chapter offers an outlook confirming that the concept of metafilm music is not confined to Godard’s work. A list provides names of other primarily modernist or postmodern filmmakers drawn to self-reflexive filmmaking and further suggests bodies of films that may likely include music that can be analyzed as metafilm music.
Impact of water flow rate on finishing pig performance
Authors: Miller Hannah E , McClellan Katlyn , Perez-Palencia Jorge Y , Samuel Ryan S , Levesque Crystal L , Thaler Robert C 
Published: September 2022

Abstract
A survey of 23 South Dakota pork producers in 2019 reported that 68% of the waterers in finishing barns had water flow rates above the recommended rate of 500–1,000 mL/min. The objective of the two studies was to determine the impact of water flow rate on finishing pig performance in the summer months. Study 1 used a total of 396 pigs in two groups in a 77-day trial (35.0 to 104.3 kg BW) with 6 pigs/pen and 1 cup waterer/pen. Study 2, conducted in a commercial style barn, used a total of 1,227 pigs in an 84-day trial (60.9 to 117.4 kg BW) with 26 pigs/pen and 2 cup waters/pen. Pens were assigned to one of three water flow rates (high, medium, low) based on the 3-hole settings of the water nipples (2.0, 1.0, and 0.8 mm; n = 22 and 16 pens/treatment for Study 1 and 2, respectively). Room temperature, outside temperature and relative humidity were recorded daily for both studies. In Study 1, water disappearance was recorded daily, and individual pen water flow rates were recorded every two weeks. At every diet phase change (26 ± 2.6 days), feed disappearance and individual pig body weights were recorded. Water flow rates averaged 1856 ± 188, 906 ± 214, 508 ± 100 mL/min for high, medium, and low flow settings, respectively. In Study 2, individual pen water flow rate, water disappearance, BW, and feed disappearance were recorded every two weeks. Water flow rates averaged 1115 ± 98, 906 ± 209, and 605 ± 203 mL/min for high, medium, and low flow settings, respectively. In both studies, there were no differences in final BW, cumulative ADG, or G:F. Due to the variability of water flow rate within a setting, data was further analyzed using regression with flow rate as the independent variable. Apart from average daily water disappearance (adj. R2 = 0.87), there was a low relationship between pig performance and water flow rate (adj. R2 &lt; 0.09). The low R2 values associated with pig performance and the high association with water disappearance suggests that water flow rate above current recommendations has little impact on finishing pig performance but does contribute to water wastage and its associated costs.
Root nitrogen uptake capacity of Chinese fir enhanced by warming and nitrogen addition
Authors: Jiang Qi , Lin Chengfang , Guo Runquan , Xiong Decheng , Yao Xiaodong , Wang Xiaohong , Chen Tingting , Jia Linqiao , Wu Dongmei , Fan Ailian , Chen Guangshui , Yang Yusheng 
Published: September 2022

Abstract
There is a knowledge gap in the effects of climate warming and nitrogen (N) deposition on root N absorption capacity, which limits our ability to predict how climate change alters the N cycling and its consequences for forest productivity especially in subtropical areas where soil N availability is already high. In order to explore the effects and mechanism of warming and the N deposition on root N absorption capacity of Chinese fir (Cunninghamia lanceolata), a subtropical arbuscular mycorrhizal conifer, the fine root 15NH4+ and 15NO3− uptake kinetics at a reference temperature of 20 °C were measured across different seasons in a factorial soil warming (ambient, +5 °C) × N addition (ambient, +40 kg N ha−1 yr−1) experiment. The results showed that (i) compared with the control, warming increased the maximal uptake rate of NH4+ (Vmax,20 °C-NH4+) in summer, while N addition enhanced it in spring and summer; compared with non-warming treatments, warming treatments increased the uptake rate of NO3− at a reference concentration of 100 μmol (V100,20 °C-NO3−) in spring. (ii) The analysis of covariance showed that Vmax,20 °C-NH4+ was positively correlated with root mycorrhizal colonization rate (MCR) and V100,20 °C-NO3− was positively correlated with specific root respiration rate (SRR), whereas no N uptake kinetic parameter was correlated with specific root length, root N and non-structural carbon concentrations. Thus, our results demonstrate that warming-increased root NH4+ uptake might be related to warming-increased MCR, whereas warming-increased root NO3− uptake might be related to warming-increased SRR. We conclude that root NH4+ and NO3− uptake capacity of subtropical Chinese fir can be elevated under warming and N deposition, which could improve plantation productivity and mitigate N leaching loss and soil acidification.
Encouragement for the Christian Nurse
Authors: Jones Christina L. Cummins 
Published: September 2022

ABSTRACT:
In today's difficult and chaotic time, nurses are reminded that their work is a calling from God. When overwhelmed and discouraged, biblical truths in this article may serve as reminders that God is present, carries burdens, comforts, and provides hope. God gives multiple spiritual resources for encouragement and enablement to do his work.
Instruments for Assessing Spiritual/Religious Coping in Multicultural Clinical Settings
Authors: Casaleiro Tiago , Caldeira Sílvia , Esperandio Mary Rute G. 
Published: September 2022

ABSTRACT:
A review of literature was conducted to discover and compare various instruments that could be used in daily nursing practice to assess spiritual and religious coping in a variety of multicultural clinical settings. Fifteen spiritual and religious (S/R) instruments were identified, used in 31 studies in multiple countries. These S/R coping tools may be useful to nurses in assessment and for planning interventions.
Demonstrating Servant Leadership During the COVID-19 Pandemic
Authors: Simon Elizabeth , Mathew Anjali Nisha , Thomas V.V. 
Published: September 2022

ABSTRACT:
Servant leadership (SL) is an especially effective leadership style for healthcare. This article relays characteristics, attributes, and essential behaviors of SL. Jesus Christ demonstrated SL by inverting the traditional leadership triangle of leader at the top. Servant leadership promotes a culture of openness, transparency, empowerment, and sharing of ownership. The servant leader values listening, developing, encouraging, building collaboration, and maintaining personal relationships, while displaying authenticity, openness, accountability, and willingness to learn. The experience of a staff nurse functioning as a servant leader for patients and colleagues during the COVID-19 pandemic elaborates how SL can be evidenced in bedside care.