bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineering tumor-specific catalytic nanosystem for NIR-II photothermal-augmented and synergistic starvation/chemodynamic nanotherapy
Authors: Zhou Shuixiu , Xu Jiahuan , Dai Yanfei , Wei Yan , Chen Liang , Feng Wei , Chen Yu , Ni Xuejun 
Published: November 2022

Abstract

Background

As an emerging therapeutic modality, chemodynamic therapy (CDT), converting hydrogen peroxide (H
2
O
2
) into highly toxic reactive oxygen species (ROS), has been developed for tumor-specific therapy. However, the deficiency of endogenous H
2
O
2
and high concentration of glutathione (GSH) in the tumor microenvironment (TME) weaken the CDT-based tumor-therapeutic efficacy. Herein, a photothermal-enhanced tumor-specific cascade catalytic nanosystem has been constructed on the basis of glucose oxidase (GOD)-functionalized molybdenum (Mo)-based polyoxometalate (POM) nanoclusters, termed as GOD@POMs.

Methods
GOD@POMs were synthesized by a facile one-pot procedure and covalently conjugation. Then, its structure was characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). In addition, ultraviolet-visible-near-infrared (UV-vis-NIR) absorption spectrum and infrared thermal camera were applied to evaluate the catalytic and photothermal performance, respectively. Moreover, to confirm the therapeutic effects in vitro, cell counting kit-8 (CCK-8) assay, live/dead staining and ROS staining were performed. Furthermore, the biosafety of GOD@POMs was investigated via blood routine, blood biochemistry and hematoxylin and eosin (H&E) staining in Kunming mice. Besides, the C6 glioma tumor-bearing mice were constructed to evaluate its anti-tumor effects in vivo and its photoacoustic (PA) imaging capability. Notably, RNA sequencing, H&E, TdT-mediated dUTP nick end labeling (TUNEL) and Ki-67 staining were also conducted to disclose its underlying anti-tumor mechanism.

Results

In this multifunctional nanosystem, GOD can effectively catalyze the oxidation of intratumoral glucose into gluconic acid and H
2
O
2
, achieving the cancer starvation therapy. Meanwhile, the generated gluconic acid decreases the pH in TME resulting in POM aggregation, which enables PA imaging-guided tumor-specific photothermal therapy (PTT), especially in the second near-infrared (NIR-II) biological window. Importantly, the Mo (VI) sites on POM can be reduced to Mo (V) active sites in accompany with GSH depletion, and then the post-produced Mo (V) transforms in situ overproduced H
2
O
2
into singlet oxygen (
1
O
2
) via Russell mechanism, achieving self-enhanced CDT. Moreover, the PTT-triggered local tumor temperature elevation augments the synergistic nanocatalytic-therapeutic efficacy.

Conclusions

Consequently, the integration of GOD-induced starvation therapy, H
2
O
2
self-supply/GSH-depletion enhanced Mo-based CDT, and POM aggregation-mediated PTT endow the GOD@POMs with remarkable synergistic anticancer outcomes with neglectable adverse effects.
Gene transcriptional profiles in gonads of Bacillus taxa (Phasmida) with different cytological mechanisms of automictic parthenogenesis
Authors: Forni Giobbe , Mikheyev Alexander S. , Luchetti Andrea , Mantovani Barbara 
Published: November 2022

AbstractThe evolution of automixis – i.e., meiotic parthenogenesis – requires several features, including ploidy restoration after meiosis and maintenance of fertility. Characterizing the relative contribution of novel versus pre-existing genes and the similarities in their expression and sequence evolution is fundamental to understand the evolution of reproductive novelties. Here we identify gonads-biased genes in two Bacillus automictic stick-insects and compare their expression profile and sequence evolution with a bisexual congeneric species. The two parthenogens restore ploidy through different cytological mechanisms: in Bacillus atticus, nuclei derived from the first meiotic division fuse to restore a diploid egg nucleus, while in Bacillus rossius, diploidization occurs in some cells of the haploid blastula through anaphase restitution. Parthenogens’ gonads transcriptional program is found to be largely assembled from genes that were already present before the establishment of automixis. The three species transcriptional profiles largely reflect their phyletic relationships, yet we identify a shared core of genes with gonad-biased patterns of expression in parthenogens which are either male gonads-biased in the sexual species or are not differentially expressed there. At the sequence level, just a handful of gonads-biased genes were inferred to have undergone instances of positive selection exclusively in the parthenogen species. This work is the first to explore the molecular underpinnings of automixis in a comparative framework: it delineates how reproductive novelties can be sustained by genes whose origin precedes the establishment of the novelty itself and shows that different meiotic mechanisms of reproduction can be associated with a shared molecular ground plan.
Morphological evolution and diversity of pectoral fin skeletons in teleosts
Authors: Tanaka Yoshitaka , Miura Hiroki , Tamura Koji , Abe Gembu 
Published: November 2022

AbstractThe Teleostei class has the most species of the fishes. Members of this group have pectoral fins, enabling refined movements in the water. Although teleosts live in a diverse set of environments, the skeletal pattern of pectoral fins in teleosts is considered to show little morphological variability. Here, in order to elucidate variations in pectoral fin skeletons and to identify their evolutionary processes, we compared the pectoral fin skeletons from 27 species of teleosts. We identified several variations and a diversity of pectoral fin skeletal patterns within some teleost groups. Taken together with previous reports on teleost skeletons, our findings reveal that in the course of teleost evolution, there are a mixture of conserved and non-conserved components in the pectoral fin skeletons of teleosts, and that teleosts may have experienced the variation and conservation of the number and shape of the proximal radials, the loss of the mesocoracoid, and the change in the distal radial-fin ray relationship.
Teaching, coaching, or debriefing With Good Judgment: a roadmap for implementing “With Good Judgment” across the SimZones
Authors: Fey Mary K. , Roussin Christopher J. , Rudolph Jenny W. , Morse Kate J. , Palaganas Janice C. , Szyld Demian 
Published: November 2022

AbstractSimulation-based learning occurs in multiple contexts, and one teaching style cannot adequately cover the needs at each learning level. For example, reflective debriefing, often used following a complex simulation case, is not what is needed when learning new skills. When to use which facilitation style is a question that educators often overlook or struggle to determine. SimZones is a framework used to clarify the multiple contexts in simulation. This framework, combined with elements of Debriefing With Good Judgment, can help educators match the appropriate facilitation style with learner needs and learning context. We have distilled the core elements of the “with good judgment” approach to debriefing and applied them to the SimZones framework to guide educators with (1) what type of learning can be expected with each learning context, (2) what behaviors and activities can be expected of the learners in each learning context, (3) what instructional strategies are most effectively used at each stage, and (4) what are the implications for the teacher-learner relationship.
High-flow continuous hemodiafiltration successfully decreased blood pregabalin levels in a patient with severe pregabalin intoxication: a case report
Authors: Shimazui Takashi , Kitamura Nobuya , Kako Kuniyuki , Iwase Shinya , Suzuki Toshinao , Hoshino Shota , Futagami Hiromi , Kibayashi Kazuhiko , Nakao Ken-ichiro 
Published: November 2022

AbstractBackgroundSevere pregabalin intoxication may cause serious symptoms, such as coma. Since pregabalin is a small molecule with no protein binding sites and has low volume of distribution, hemodialysis can be effective in eliminating pregabalin from the blood. However, in cases of emergency, it is not always possible to perform hemodialysis because of limited availability and time delay associated with using the plumbing equipment. Continuous hemodiafiltration (CHDF) can be performed without plumbing equipment; however, the knowledge on the effectiveness of CHDF in pregabalin elimination is insufficient.Case presentationA septuagenarian woman with normal renal function was found in a collapsed state with symptoms of coma and miosis. Empty medical bags of pregabalin (2350 mg), bepotastine besilate (600 mg), celecoxib (4600 mg), quetiapine fumarate (87.5 mg), clotiazepam (180 mg), and teprenone (50 mg) were found around her. During the patient's transfer to our hospital, her cognition worsened and she developed glossoptosis necessitating her emergent intubation upon arrival. We considered that the coma was mainly caused by pregabalin intoxication and were concerned about the consequent critical comorbidities. Thus, we performed CHDF in a high-flow setting in our intensive care unit for pregabalin elimination. After 8 h of CHDF, the patient regained consciousness, and after 6.5 h we extubated her. At a later date, we measured her serum pregabalin levels during the clinical course and estimated the blood pregabalin clearance levels depending on her metabolism as 76.8 mL/min and depending on CHDF itself as 65.1 mL/min. Based on these findings, we concluded that CHDF contributed to reducing blood pregabalin levels in this patient.ConclusionsOur case revealed that pregabalin clearance using CHDF is similar to metabolic clearance in patients with normal renal function, indicating that CHDF decreases blood pregabalin levels and can be a potential treatment for severe pregabalin intoxication.
On intersectionality: visualizing the invisibility of Black women
Authors: Billups Shelby , Thelamour Barbara , Thibodeau Paul , Durgin Frank H. 
Published: November 2022

AbstractIntersectionality refers to the simultaneous and interacting effects of multiple group categorization on individuals with minoritized status, often leading to being perceived in a manner inconsistent with the additive contributions of those categories. For Black women, a number of findings have contributed to the idea that Black women have a unique perceived absence of status, for example, and are perceived as distinct from being Black or a woman. We sought to quantify and visualize the combined effects of race and gender on judgments of persons using data-defined dimensions (the Semantic Differential; Osgood et al. in The measurement of meaning, University of Illinois Press, Champaign, 1957). Our data suggest that gender and race contribute to orthogonal dimensions of difference in the perception of persons. Whereas white males, white females, and Black males all seem to be perceived in accord with additive effects in these two dimensions, Black females seem to be perceived more neutrally, as if neither their gender nor their race is treated as predictive.
Comparability of a provisioned device versus bring your own device for completion of patient-reported outcome measures by participants with chronic obstructive pulmonary disease: quantitative study findings
Authors: Hudgens Stacie , Newton Louise , Eremenco Sonya , Crescioni Mabel , Symonds Tara , Griffiths Philip C. G. , Reasner David S. , Byrom Bill , O’Donohoe Paul , Vallow Susan ,   the Patient-Reported Outcome (PRO) Consortium and Electronic Clinical Outcome Assessment (eCOA) Consortium
Published: November 2022

Abstract
Objective
To quantitatively compare equivalence and compliance of patient-reported outcome (PRO) data collected via provisioned device (PD) versus bring your own device (BYOD).

Methods
Participants with stable chronic obstructive pulmonary disease (COPD) completed the EXAcerbations of Chronic Pulmonary Disease Tool (EXACT®) daily and COPD Assessment Test™ (CAT) and Patient Global Impression of Severity (PGIS) of COPD weekly on either PD or BYOD for 15 days, then switched device types for 15 days. EXACT was scored using the Evaluating Respiratory Symptoms in COPD (E-RS®: COPD) algorithm and equivalence assessed using intraclass correlation coefficients (ICCs) adjusting for cross-over sequence, period, and time. Two one-sided tests (TOSTs) used ICC adjusted means with 10%, 20%, and 40% of total score tested as equivalence margins. Compliance and comfort with technology were assessed. Equivalence across 3 device screen sizes was assessed following the second completion period.

Results
Participants (N = 64) reported high comfort with technology, with 79.7% reporting being “quite a bit” or “very” comfortable. Weekly compliance was high (BYOD = 89.7–100%; PD = 76.9–100%). CAT and E-RS: COPD scores correlated well with PGIS (r > 0.50) and demonstrated equivalence between PD and BYOD completion (ICC = 0.863–0.908). TOST equivalence was achieved within 10% of the total score (p > 0.05). PRO measure scores were equivalent across 3 different screen sizes (ICC = 0.972–0.989).

Conclusions
Measure completion was high and scores equivalent between PD and BYOD, supporting use of BYOD in addition to PD for collecting PRO data in COPD studies and in demographically diverse patient populations.
Fully automated method for dental age estimation using the ACF detector and deep learning
Authors: Pintana Patipan , Upalananda Witsarut , Saekho Suwit , Yarach Uten , Wantanajittikul Kittichai 
Published: November 2022

Abstract
Background
Dental age estimation plays an important role in identifying an unknown person. In forensic science, estimating age with high accuracy depends on the experience of the practitioner. Previous studies proposed classification of tooth development of the mandibular third molar by following Demirjian’s method, which is useful for dental age estimation. Although stage of tooth growth is very helpful in assessing age estimation, it must be performed manually. The drawback of this procedure is its need for skilled observers to carry out the tasks precisely and reproducibly because it is quite detailed. Therefore, this research aimed to apply computer-aid methods for reducing time and subjectivity in dental age estimation by using dental panoramic images based on Demirjian’s method. Dental panoramic images were collected from persons aged 15 to 23 years old. In accordance with Demirjian’s method, this study focused only on stages D to H of tooth development, which were discovered in the 15- to 23-year age range. The aggregate channel features detector was applied automatically to localize and crop only the lower left mandibular third molar in panoramic images. Then, the convolutional neural network model was applied to classify cropped images into D to H stages. Finally, the classified stages were used to estimate dental age.

Results
Experimental results showed that the proposed method in this study can localize the lower left mandibular third molar automatically with 99.5% accuracy, and training in the convolutional neural network model can achieve 83.25% classification accuracy using the transfer learning strategy with the Resnet50 network.

Conclusion
In this work, the aggregate channel features detector and convolutional neural network model were applied to localize a specific tooth in a panoramic image and identify the developmental stages automatically in order to estimate the age of the subjects. The proposed method can be applied in clinical practice as a tool that helps clinicians to reduce the time and subjectivity for dental age estimation.
Comparative study of the efficacy of ultrasound-guided erector spinae block and oblique subcostal transversus abdominis plane block for postoperative analgesia after laparoscopic cholecystectomy
Authors: Engineer Smita R. , Devanand Asha , Kulkarni Mrinalini 
Published: November 2022

Abstract
Background
Laparoscopic cholecystectomy has changed the surgical management of cholelithiasis and has become the mainstay of the management of uncomplicated gallstone disease. Adequate postoperative pain relief leading to early ambulation is imperative for patient satisfaction and early discharge of the patient. The use of ultrasound in anesthetic practice has ushered in a new era of ultrasound-guided blocks for postoperative analgesia, replacing the conventional methods. This study compares two modalities of postoperative pain relief, namely the oblique subcostal transversus abdominis plane block and the newer erector spinae plane block for patients undergoing laparoscopic cholecystectomy.

Results
Sixty patients between the age group 18 to 75 of ASA grades I, II, and III were enrolled in the study. The erector spinae plane block group showed lower numerical rating scores up to 12 h, a longer time period for the requirement of first rescue analgesic, and lower total analgesic consumption postoperatively compared to the oblique subcostal transversus abdominis plane block group. Both blocks were found to have minimal side effects.

Conclusions
The erector spinae plane block is superior to the oblique subcostal transversus abdominis plane block in that it affords lower pain scores and a longer duration of analgesia and reduces the total analgesic consumption after laparoscopic cholecystectomy.

Trial registration
Clinical Trials Registry of India/CTRI/2020/10/028603/ registered on 23 October 2020
http://ctri.nic.in/Clinicaltrials/rmaindet.php?trialid=47807&EncHid=18303.55562&modid=1&compid=19
“Preoperative oral duloxetine: does it affect duration of spinal anesthesia and early postoperative pain after arthroscopic ACL repair?” A prospective, randomized, double-blind controlled trial
Authors: Abdelaziz Tamer Samir Abdelsalam , Mahmoud Ismail Mohammed Ibrahim 
Published: November 2022

Abstract
Background
Postoperative pain is one of the problems in which a lack of adequate controls can cause many complications. Duloxetine is a potent serotonin and norepinephrine reuptake inhibitor (SNRI) prescribed for the treatment of depression, chronic pain, neuropathy, and recently early postoperative pain.

Results
The results showed that the effect of duloxetine on the onset and duration of the spinal anesthesia was statistically non-significant (P = 0.067 and P = 0.21) respectively; also, duloxetine delayed the time to the first dose of rescue analgesia request (479.71 ± 50.32 vs 218.29 ± 12.48) (P < 0.001) and maintained VAS score in the lower range in comparison to control group (P = 0.001) with less frequency and total morphine consumption (4.2 ± 2.08 vs 10.37 ± 1.52) (P < 0.001) up to 24 h. No significant differences in adverse effects.

Conclusions
A single dose of 60 mg duloxetine orally 2 h before arthroscopic ACL repair provided better postoperative pain control and decreased total morphine consumption without affecting the duration of spinal anesthesia.
Chemical characterization and antimicrobial activity of Blumenbachia insignis (Loasaceae) native to South America
Authors: Fangio Maria Florencia , Herrera Gonzalo Burgos , Medici Sandra , Cardinali Francisco , Ramirez Cristina Lujan 
Published: November 2022

Abstract
Background
Blumenbachia insignis is a plant native to Brazil, Uruguay, and Argentina. It is used as an ornamental plant for its flowers, but also, in popular medicine, the stems and leaves are used as antirheumatic agents. Detailed studies on the differential chemical composition of their organs were carried out to establish a relationship with their biological activity and ethnomedicinal uses and the compounds present.

Results
The presence of phenolic compounds, flavonoids, and triterpenoid saponins was detected in the extracts of the different organs of Blumenbachia insignis, those of the flower being the ones that had the highest concentration of these families of compounds, and also the highest antioxidant and antimicrobial activity. Volatile compounds were identified using gas chromatography coupled to mass spectrometry (GC–MS) in all the extracts. Some of them possess recognized antimicrobial and antioxidant activity among others. On the other hand, the roots showed an important presence of monoterpenes, not so common for these organs.

Conclusions
These characteristics could be useful to prevent various oxidative stress processes and against pathogenic bacteria.
Taste characteristics, volatile components, sensory properties, and antioxidant activity of fresh onion (Allium cepa L.) leaves
Authors: Yuasa Masahiro , Ueno Mayuko , Kawabeta Koji , Morikawa Maho , Uemura Momoe , Matsuzawa Tetsuhiro , Tominaga Mihoko 
Published: November 2022

Abstract
Background
To evaluate the usefulness of fresh onion (Allium cepa L.) leaves, a food waste, as a new food resource, we characterized the taste characteristics, volatile compounds, sensory properties, and antioxidant activity of fresh onion leaves compared with those of Welsh onion (Allium fistulosum L.) (green leaf, small variety) leaves.

Results
The total sugar (g/100 g), total organic acid (mg/100 g), and total free amino acid (mg/100 g) concentrations of fresh onion leaves were 2.12 ± 0.15, 730.02 ± 17.43, and 93.72 ± 7.17, respectively, and were significantly (p < 0.05) lower than those of Welsh onion leaves (2.38 ± 0.07, 907.23 ± 20.79, and 131.34 ± 10.22, respectively). In fresh onion leaves, dipropyl disulfide concentration was higher than five times that in Welsh onion leaves. Regarding the sensory properties of the boiled samples, the fresh onion leaves’ color was less favorable than Welsh onion leaves (p < 0.05), and the taste and aroma were indifferent. The total oxygen radical absorbance capacity value (μmol Trolox equivalents/100 g) and 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging activity (μmol Trolox equivalents/100 g) of fresh onion leaves were 805.78 ± 100.32 and 406.70 ± 63.64, respectively, and differed insignificantly compared with Welsh onion leaves (888.00 ± 112.61 and 382.98 ± 26.08, respectively). However, the total phenolic content (mg gallic acid equivalents/100 g) and quercetin concentration (mg/100 g) of fresh onion leaves were 36.53 ± 2.53 and 5.71 ± 0.11, respectively, and were significantly (p < 0.05) higher than those of Welsh onion leaves (25.07 ± 2.02 and 0.18 ± 0.02, respectively). Additionally, total vitamin C (23.36 ± 0.62 mg/100 g) and β-carotene (1529.32 ± 167.77 μg/100 g) concentrations in fresh onion leaves were similar to those rich vegetables.

Conclusions
This is the first study to report fresh onion leaves’ sensory properties and volatile compounds. Additionally, fresh onion leaves could be a new food resource, presenting an alternative to Welsh onion leaves and acting as an excellent dietary source of antioxidants, including quercetin, vitamin C, and β-carotene.
The wandering spleen: case report of laparoscopic splenectomy in a pregnant woman
Authors: Crepaz Lorenzo , Di Leo Alberto , Borzellino Giuseppe , Pomini Paola , Genna Michele 
Published: November 2022

Abstract
Background
Wandering spleen is a rare condition, which is characterized by augmented mobility of the spleen, due to congenital or acquired causes. It is more frequent in multiparous women, but only a few cases are reported during pregnancy. Wandering spleen is usually asymptomatic until the onset of its possible complications, and this can mislead clinicians in reaching the correct diagnosis.

Case presentation
We report the case of a wandering spleen with acute splenic infarction in a pregnant woman and its minimally invasive surgical treatment, focusing on the clinical and radiological findings that could mislead or drive clinicians to the right diagnosis, or a potential disaster.

Conclusions
Splenic preservation in the wandering spleen where it is safe and possible is the main goal of the treatment. Clinical and radiological findings are the most relevant elements to drive surgical treatment. To reach the best and opportune treatment for the patient, the wandering spleen is an insidious condition and must be included in the differential diagnosis in the acute abdomen diagnostic process.