bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Ingenious Strategy to Stereoscopically Enhance the Phosphorescence Properties of Guest–Host Materials: Addition of Metal Ions to Form Coordination with the Guest
Authors: Guo Yan , Cheng Kaijun , Hu Zechen , Lei Yunxiang , Liu Xiaoqing , Liu Miao-Chang , Cai Zhengxu , Wu Huayue , Huang Xiaobo , Dong Yuping 
Published: March 2022

Abstract
The host–guest doped strategy has been widely recognized to be effective for preparing room-temperature phosphorescence materials. However, the advantage of the host in a doped system as a container that can hold the other components is ignored. In this study, benzophenone was selected as the host, and seven phenylquinoline isomers were designed as guests to construct a phosphorescence-doped system. Notably, seven metal salts of elements in the same period or in the same main group (e.g., Al3+, Cu+/2+, Zn2+, Ga3+, Ag+, Cd2+, and In3+, respectively) are selected as the third component to coordinate with the nitrogen atom of the guests, thereby stereoscopically improving the phosphorescence performance of the doped system. Metal ions can significantly enhance the phosphorescence intensity, cause red-shift of the emission wavelength, and prolong the phosphorescence lifetime of doped materials. Ag+ and Cd2+ can considerably increase the emission intensity up to 38 times, and the highest phosphorescence quantum efficiency reaches 70%. Al3+, Ga3+, and In3+ can prolong the emission wavelength, and the phosphorescence wavelength can be red-shifted up to 60 nm. Cu2+, Ga3+, and In3+ can extend the phosphorescence lifetime by a maximum of 3.6 times.
An Ideal Neonatal Congenital Heart Disease Screening Plan by Pulse Oximeter and Auscultation in a Large Single Center
Authors: Huang Yuqiang , Zhong Shiqing , Zhang Xianmei , Kong Linghui , Wu Wenli , Yue Shixia , Tian Ning , Zhu Guanghua , Hu Aiqin , Xu Juan , Zhu Haijan , Sun Airong , Qin Fangling , Wang Ziwen , Wu Shiqiang 
Published: March 2022

Abstract
Purpose
To investigate the feasibility and reliability of pulse oximeter (POX) as a fast-screening device for congenital heart disease (CHD) through comparing performances of cardiac auscultation and POX in a large retrospective single center study.
Methods
Every newborn received auscultation and POX tests separately. When an abnormal murmur or SpO2 level is detected, an echocardiogram will be ordered to confirm the diagnosis of CHD in the newborn.
Results
From January 1, 2018 to December 31, 2019, there were 44,147 livebirths at the studied hospital where 498 newborns were diagnosed with CHD: 27 newborns by POX screening and 471 by cardiac auscultation. The diagnosis was further confirmed in 458 neonates through echocardiogram. This result puts forth an overall diagnosis rate of 91.97%. Cardiac auscultation detected the majority of CHD cases 438 (95.6%) while POX only screened 20 (4.4%) cases. Interestingly, no CHD case was detected by both auscultation examination and POX screening. Auscultation detected most of the common types of CHD, but POX excelled in identifying rare and critical cases. POX screening alone had a very low accuracy of 3.41% in positive predict value (PPV). On the other hand, auscultation can function well in terms of PPV and negative predict value (NPV) (92.99% and 99.95%, respectively), but the addition of POX could improve the overall screening performance and reach NPV to 100%. We also validate the finding with the data six months after the study period.
Conclusion
Auscultation screening operates well in detecting common CHDs whereas POX screening excels in distinguishing rare, yet critical, cases of CHDs. Therefore, an ideal CHD screening plan should combine cardiac auscultation and POX screening.
Mid-band PPN InGaN solar cell for indoor visible light communication in 5G/6G applications
Authors: Manzoor Habib Ullah , Shiong Ng Sha , Aslam Arooj , Manzoor Tareq 
Published: March 2022

Abstract
Recently, solar cells have been simultaneously used as energy harvester and receiver in free-space optical (FSO) communication networks. In this study, a mid-band p-In0.01Ga0.99N/p-In0.5Ga0.5N/n-In0.5Ga0.5N (PPN) solar cell that achieved a conversion efficiency of 26.36% (under 1.5 AM condition) was used as a receiver for an indoor FSO communication network. Considering that this solar cell had a length and width of 1 mm, the FSO system was simulated using Optisystm software. Meanwhile, the solar cell was simulated using SCAPS-1D software. The power received from the solar cell was compared with those of four commercially available avalanche photodiode (APD) receivers. Incident wavelength was varied in the visible region from 400 nm to 700 nm for transmission lengths of 5, 10, 15, and 20 m. The current–voltage and power–voltage curves were presented at different incident wavelengths and transmission distances. The InGaN solar cell provides more electrical power than all the commercial APDs. In conclusion, an increase in received power can improve the quality of an FSO network and support longer transmission length.
A 5’ UTR Mutation Contributes To Down-Regulation Of Bbs7 In The Berlin Fat Mouse
Authors: Mohebian Kourosh , Hesse Deike , Arends Danny , Brockmann Gudrun A. 
Published: March 2022

Abstract
The Bardet Biedl syndrome 7 (Bbs7) gene was identified as the most likely candidate gene causing juvenile obesity in the Berlin Fat Mouse Inbred (BFMI) line. Bbs7 is significantly lower expressed in brain, adipose tissue, and liver of BFMI mice compared to lean C57BL/6NCrl (B6N) mice. DNA sequence comparison between BFMI and B6N revealed 16 sequence variants in the Bbs7 promoter region. Here, we tested if these mutations contribute to the observed differential expression of Bbs7.In a cell-based dual luciferase assay we compared the effects of the BFMI and the B6N haplotypes of different regions of the Bbs7 promotor on the reporter gene expression. A single SNP was identified causing a significant reduction in the reporter gene expression. This SNP (rs29947545) is located in the 5’ UTR of Bbs7 at Chr3:36.613.350. The SNP is not unique to BFMI mice, but occurs also in several other mouse lines, where the BFMI allele is not associated with lower Bbs7 transcript amounts. As such, a second factor alongside the SNP rs29947545 or interacting with the SNP location is likely required to cause the reduction of Bbs7 expression which leads to the obese phenotype in BFMI mice.
Global phosphoproteomic profiling of skeletal muscle in ovarian-hormone deficient mice
Authors: Peyton Mina P. , Yang Tzu-Yi , Higgins LeeAnn , Markowski Todd W. , Vue Cha , Parker Laurie L. , Lowe Dawn A. 
Published: March 2022

Abstract
Background
Phosphorylation is important in skeletal muscle development, growth, regeneration, and contractile function. Alterations in the skeletal muscle phosphoproteome due to aging have been reported in males; however, studies in females are lacking. We have demonstrated that estrogen deficiency decreases muscle force which correlates with decreased myosin regulatory light chain phosphorylation. Thus, we questioned whether the decline of estrogen in females that occurs with aging might alter the skeletal muscle phosphoproteome.
Methods
C57BL/6J female mice randomly assigned to a sham-operated (Sham) or ovariectomy (Ovx) group to investigate the effects of estrogen deficiency on skeletal muscle protein phosphorylation in a resting, non-contracting condition. After 16 weeks of estrogen deficiency, the tibialis anterior muscle was dissected and prepped for label-free nano-liquid chromatography tandem mass spectrometry phosphoproteomic analysis.
Results
We identified 4,780 phosphopeptides in tibialis anterior muscles of ovariectomized (Ovx) and Sham-operated (Sham) control mice. Further analysis revealed 647 differentially regulated phosphopeptides (Benjamini – Hochberg adjusted p-value < 0.05 and 1.5-fold change ratio) that corresponded to 130 proteins with 22 proteins differentially phosphorylated (3 unique to Ovx, two unique to Sham, six upregulated, and 11 downregulated). Differentially phosphorylated proteins associated with the sarcomere, cytoplasm, and metabolic and calcium signaling pathways were identified.
Conclusion
Our work provides the first global phosphoproteomic analysis in females and how estrogen deficiency impacts the skeletal muscle phosphoproteome. Future work into specific phosphorylation events on sarcomeric proteins and phosphoproteins pertaining to calcium signaling may offer insight on the molecular mechanism(s) of skeletal muscle strength loss in females.
Traditional Knowledge of Wild Plant Species Used by Local People Inhabiting the Southern Part of Wadi Araba Desert in South-West Jordan
Authors: Albalawneh Abeer , Nawash Oraib , Al-Assaf Amani , Hjazin Amjad , Tadros Maher , Kabariti Rabab , Nowar Lana Abu , Albashbsheh Ghadeer , Diab Mai , Aljaafreh Safaa , Hammour Wafa’a Abu , Alwidyan Jafar , Hadad Nizar 
Published: March 2022

Abstract
BackgroundEthnobotanical research studies in Jordan mostly targeted the medicinal uses of plants ignoring the other important uses. Comprehensive documentation of traditional knowledge of using native plants in the delicate desert ecosystem is very crucial for prioritizing biodiversity conservation and socio-economic improvement programs. This study therefore documents and analyzes the traditional knowledge of native plants used for grazing, firewood, food, and medicine by local inhabitants of the southern part of Wadi Araba desert in southwest Jordan. MethodsThe information was obtained using a structured survey targeting 296 informants inhabiting the southern part of Wadi Araba. The documented data was quantitively analyzed by calculating the frequency of citation for the uses categories and species used. ResultsThe results showed that the most important species used as a source of feed for the grazing animals and for firewood is Haloxylon persicum, which also indicates that the species is under overexploitation stress. About 91% of the informants mentioned that they collect plants for medicinal uses compared to 7% who collect them for food, and 6.4% of the informants mentioned that they get the plants from their families and they rarely cultivate them. The informants attributed the reduction in vegetation cover to the lack of water and overgrazing. Whereas, firewood scarcity and long distance to the grazing and wood cutting sites are the most obstacles that face firewood collection. ConclusionOur study concluded that the local people of the study area are still interested in the utilization of the native plants in their surroundings as their ancestors did. Yet, there is some pressure on certain plant species and certain geographic areas that conservation and development programs should take into consideration. This information obtained in this study is interesting for economic improvement and biodiversity conservation programs in adopting small businesses that cultivate the most collected plants to reduce pressure on the ecosystem and to generate income for local families.
A 3-year retrospective study of 866 children and adolescent outpatients followed in the Nice Pediatric Psychotrauma Center created after the 2016 mass terror attack
Authors: Gindt Morgane , Fernandez Arnaud , Ménard Mariline , Nachon Ophélie , Richez Aurélien , Auby Philippe , Battista Michèle , Askenazy Florence 
Published: March 2022

Abstract
Background
The mass terrorist attack in Nice, France in July 2016 caused deaths and injuries in a local population, including children and adolescents. Within six months, the Nice Pediatric Psychotrauma Center was opened to provide mental health care to the pediatric population (0–18 years) who experienced traumatic events.
Objectives
To report clinical findings and provide considerations focusing on 1) Traumatic events; 2) Psychiatric diagnoses; 3) Needed care of pediatric population; 4) Recommendations based on the experience of Nice Pediatric Psychotrauma Center (NPPC).
Methods
In this retrospective study, we conducted quantitative and qualitative data collection and analysis of new and follow-up consultations, main and comorbid diagnoses, and the type of trauma (terrorist attack versus other potentially traumatic events). Ethics approval was obtained from the local Ethics committee.
Results
866 children and adolescents were followed in the NPPC. A high number of children and adolescents impacted by the terrorist attack required follow-up consultations (average 30 consultations per week). The first care-seeking requests after exposure to the mass terrorist attack continued to occur three years later, although at a lower rate than in the first and second years. New consultations for other traumatic events were observed over time. We found a high rate of Post-Traumatic Stress Disorder (PTSD; 71%) in this population with a high rate of comorbidities (67%), mainly sleep disorders (34.7%) and mood and anxiety disorders (16.2%).
Discussion
This study supports previous findings on the significant impact of mass trauma in pediatric population showing even a higher level of PTSD and a high rate of comorbidities. This may be explained by the brutality of the traumatic event, particularly, for this age group. The findings of this study have implications for early interventions and long-term care for children and adolescents to prevent the development of chronic PTSD into adulthood.
Construction of polycyclic aromatic hydrocarbon emission inventories in major cities in Jiangsu Province
Authors: Zhang Pei , zhou Ying , Chen Ying , Yu Miaotong , xia zhonghuan 
Published: March 2022

Abstract
The sources of 16 polycyclic aromatic hydrocarbons (PAHs) in Nanjing were studied. The overall amount for these 16 PAHs in 2019 in Nanjing was 293.54t, with 13.70t identified as carcinogenic PAHs. Most of the PAHs were generated from the combustion of Petrol, Diesel, and coke-production coal, accounting for 82.71% of the total emission amount. Among the emissions of these 16 PAHs, two- and there- ring PAHs made up the majority (81.09%). In the different areas (11 in total) of Nanjing, the PAH emissions ranged from 45.056t in Jiangning County to 10.258t in Yuhuatai County. The estimated 16 PAH emissions in Nanjing from 2005 to 2019 based on historical energy consumption data showed that the amount of PAH emission has been gradually increasing.
Decompression only versus fusion in octogenarians with spinal epidural abscesses: early complications, clinical and radiological outcome with two years follow-up
Authors: Lenga Pavlina , Gülec Gelo , Bajwa Awais Akbar , Issa Mohammed , Oskouian Rod , Chapman Jens , Kiening Karl , Unterberg Andreas , Ishak Basem 
Published: March 2022

Abstract
Despite increased life expectancy due to healthcare quality improvements globally, pyogenic vertebral osteomyelitis (PVO) treatment with a spinal epidural abscess (SEA) remains challenging in patients older than 80 years. We aimed to assess octogenarians for PVO prevalence with SEA and compare after-surgery clinical outcomes of decompression and decompression and instrumentation. A retrospective review of electronic medical records at a single institution was conducted between September 2005 and December 2020. Patient demographics, surgical characteristics, complications, hospital course, and 90-day mortality were collected. Comorbidities were assessed using the age-adjusted Charlson comorbidity index (CCI). Over 16 years, 35 patients aged ≥80 years with PVO and SEA were identified. Eighteen patients underwent surgical decompression (“decompression group”), and 17 underwent surgical decompression with instrumentation (“instrumentation group”). Both groups had a CCI > 6 (mean ± SD, 8.9 ± 2.1 vs. 9.6 ± 2.7, respectively; p = 0.065). Instrumentation group patients had a significantly longer hospital stay but no ICU stay. In-hospital and 90-days mortality rates were similar in both groups. The mean follow-up was 26.6 ± 12.4 months. No further surgeries were performed. Infection levels and neurological status were improved in both groups at discharge. At the second-stage analysis, significant improvements in the blood infection parameters and the neurological status were detected in the decompression group. Octogenarians with PVO and SEA have a high adverse events risk after surgical procedures. Surgical decompression might contribute to earlier clinical recovery in older patients. Thus, the surgical approach should be discussed with patients and their relatives and be carefully weighed.
Effect of treadmill walking on prefrontal cortex activation and dual-task performance in older adults
Authors: Baek Chang Yoon , Kim Hyeong Dong , Yoo Dong Yup 
Published: March 2022

Abstract
Background
Older adults have higher prefrontal cortex (PFC) activation and lower dual-task performance than younger adults because of age-related physical and cognitive functional impairments.
Objective
To investigate the effect of treadmill walking on PFC activation and dual-task performance in older adults.
Methods
A total of 20 older adults (mean age, 64.35 ± 2.74 years) and 20 younger adults (mean age, 30.00 ± 3.15 years) performed single- and dual-task walking in overground and treadmill conditions. A wearable functional near-infrared spectroscopy device and gait analyzer were used to analyze PFC activation and dual-task cost (DTC).
Results
In both groups, treadmill walking demonstrated reduced PFC activation while dual-task walking and reduced DTC compared to overground walking. Furthermore, despite a higher DTC in gait variability, correct response, total response, response index and a higher error score in older adults than in younger adults during overground walking, there was no difference in treadmill walking. The difference in PFC activation between single and dual tasks was also observed only in overground walking. There was no difference in PFC activation between the two age groups.
Conclusions
Older adults can maintain the similar level of dual-task performance as younger adults in treadmill walking, with PFC activation reduced. However, although older adults recruit PFC level similar to that in younger adults in overground walking, older adults show lower dual-task performance than younger adults due to inefficient use of PFC with aging. This indicates that treadmill and overground walking do not share similar activation patterns during dual-task walking.
Membrane-anchored and tumor-targeted Il-12(attIL12) PBMC therapy for osteosarcoma
Authors: Yang Qing , Hu Jiemiao , Jia Zhiliang , Wang Qi , Wang Jing , Dao LongHoang , Zhang Wendong , Xia Xueqing , Gorlick Richard , Li Shulin 
Published: March 2022

Abstract
Background
Chimeric antigen receptor (CAR) T cell therapy has shown great promise for hematologic malignancies but requires a long duration of T-cell expansion, is associated with severe toxicity, and has limited efficacy for treating solid tumor.
Methods
To address these challenges, we generated a cell membrane-anchored and tumor-targeted interleukin-12 (attIL12) to arm peripheral blood mononuclear cells (PBMCs) instead of T cells to omit the expansion phase for required CAR-T cells.
Results
This attIL12-PBMC therapy showed significant antitumor efficacy in both heterogeneous osteosarcoma patient-derived xenograft (PDx) tumors and metastatic osteosarcoma tumors with no observable toxic effects. Mechanistically, attIL12-PBMC treatment resulted in tumor-restricted antitumor cytokine release and accumulation of attIL12-PBMCs. It also induces terminal differentiation of osteosarcoma cells into bone like cells to impede tumor growth.
Conclusion
In summary, attIL12-PBMC therapy is safe and effective against osteosarcoma. Our goal is to move this treatment into a clinical trial. Owing to the convenience of the attIL12-PBMC production process, we believe it will be feasible.
Psychometric Properties of the Patient Reported Outcomes Measurement Information System (PROMIS) Scales in Acute Intermittent Porphyria Patients
Authors: Naik Hetanshi , Montgomery Guy H. , Overbey Jessica R. , Winkel Gary , Anderson Karl E. , Balwani Manisha , Wang Bruce , McGuire Brendan , Bonkovsky Herbert L. , Keel Sioban , Levy Cynthia , Erwin Angelika , Phillips John D. , Desnick Robert J. 
Published: March 2022

Abstract
Background: Acute Intermittent Porphyria (AIP) is a rare inborn error of heme biosynthesis characterized clinically by life-threatening acute neurovisceral attacks. Few studies have assessed quality of life (QoL) tools in the AIP population, and none are validated for use in AIP. In this study, the Patient Reported Outcomes Measurement Information System 57 (PROMIS-57) scales were assessed in AIP patients to determine the factor structure of PROMIS items and to compare this to previously proposed factor structures in the general population.Methods: Baseline data from the Porphyrias Consortium’s Longitudinal Study of the Porphyrias was obtained for 259 AIP patients. This included detailed disease and medical history information as well as PROMIS-57 data. Exploratory and confirmatory factor analyses (EFA and CFA, respectively) were conducted on baseline PROMIS-57 data. CFA was used to test the hypothesized three-factor structure from the PROMIS literature. Results: Internal consistency was high for all the PROMIS scales assessed. The EFA revealed a five-factor model, each consisting of a separate domain: pain interference, anxiety, depression, sleep disturbance, and fatigue. Model fit was good overall. A CFA of the hypothesized three-factor model did not converge suggesting an inappropriate structural model. Conclusion: The EFA showed a five-factor model that fit the data well, however a CFA of the three-factor model observed in the general population did not fit the data well. These findings, together with previous studies assessing correlations with clinical features, indicate that the PROMIS scales may be valid and reliable measures for AIP patients. However, further analyses are needed to confirm this. Further studies should assess correlations with other tools, whether the PROMIS scales are responsive to treatment, if they capture QoL longitudinally, and a CFA in a second sample.
An Anoikis-related Gene Signature for Forecasting Prognosis, Tumor Microenvironment, and Treatment Response in Hepatocellular Carcinoma
Authors: Chen Junliang , Liu Zhihao , Wang Huaitao , Zhou Lei , Tan Xiaodong 
Published: March 2022

Abstract
Background: As hepatocellular carcinoma (HCC) is molecularly heterogeneous, this study aimed to generate an anoikis-related gene signature for prognosis prediction and precision medication. Methods: In the TCGA-LIHC cohort, anoikis-related genes with prognostic values were determined by univariate Cox regression, followed by prognostic model construction based on the LASSO-Cox method. Independent prognostic factors were integrated to develop a nomogram. The diagnostic performances of the established model and the nomogram were assessed in training (TCGA-LIHC) and validation (GSE14520 and ICGC-LIRP-JP) cohorts using time-dependent receiver operating characteristic curve (ROC), Kaplan-Meier (KM) plot, calibration plot, principal components analysis (PCA), concordance index, and decision curve analysis (DCA). Seven published methods such as CIBERSORT were used to analyze the tumor microenvironment. The drug sensitivity was analyzed using Tumor Immune Dysfunction and Exclusion (TIDE) algorithm, IMvigor210 cohort, CellMiner database, and "oncoPredict" package. Results: Eight genes were included in the signature, including PTRH2, ITGAV, BSG, DAP3, SKP2, LGALS3, SFN, and PLK1, which could successfully divide patients into two subgroups with survival differences. The nomogram comprised of the TNM stage and our gene signature could further improve predictive accuracy. The patients in the low-risk group could benefit less more from immunotherapy, and this gene signature was also conducive to tailoring chemotherapy and molecular-targeted therapy. Conclusions: The anoikis-related gene signature provides reliable evidence for prognosis prediction, treatment optimization, and new therapeutic targets for HCC patients.