bluuebunny/crossref_metadata_2025_split huggingface dataset:
The results of treatment of the congenital vertical talus by Dobb`s method
Authors: Grzegorzewski Andrzej , Lipiński Łukasz , Pruszczyński Błażej , Grzegorzewski Paweł , Buchcic Piotr 
Published: June 2022

Abstract
Congenital vertical talus is a rare foot deformity. The hindfoot is valgus and equinus, the midfoot is dorsiflexed and forefoot is abducted caused by a fixed dorsal dislocation of the navicular on the head of the talus and the cuboid on the anterior part of the calcaneus. The epidemiology and ethiology of vertical talus is unknown. Dobbs et al in 2006 described a minimally invasive alternative avoiding the need for extensive soft tissue release procedures in treatment a congenital vertical talus. The study material contains group of 11 congenital vertical talus feet (group 5 according to Hamanishi) in 8 children (4 boys and 4 girls). The age at the time of the diagnosis varied from 5 to 26 months (mean – 14.6). Treatment involved serial manipulation and casting and then a minimally invasive approach with temporary stabilization of the talonavicular joint by K-wire and Achilles tenotomy. On lateral radiographs measurements include the talocalcaneal angle, tibiotalar angle and talar axis – first metatarsal base angle (TAMBA) and on AP radiographs - the talocalcaneal angle and talar axis – first metatarsal angle. The Wilcoxon test was used for comparison the dependent variables. The final clinical assessment during last follow up (mean 35.8 months, range 25–52) revealed in 10 cases neutral position of the foot and normal range of motion and in one case recurrence of foot deformity. Also X-ray assessment showed normalization all of the radiological parameters except for one case and were statistically significant. The minimally invasive technique described by Dobb`s should be the first option when treating the congenital vertical talus. It allows for reducing the talonavicular joint, gives good results and preserves a mobile foot. The attention should be put on early diagnosis.
Investigation of Anti-Epithelial-Mesenchymal Transition and Anti-Inflammation effects of Qing Fei Hua Xian Decotion on Pulmonary Fibrosis via Network Pharmacology and Experimental Verification
Authors: Yu Chao-chao , Li Rui-Jie , Wang Xiao-Fei , Wu Chao-Yan , Wang Xi , Peng Yan-Fang , Wang Xiu-Ping , Chen Wei , Ruan Lian-Guo , Zhang Ying-Wen 
Published: June 2022

Abstract
Background
Pulmonary fibrosis (PF) is a common interstitial pneumonia disease, also occurred in post-COVID-19 survivors. The mechanism underlying the anti-PF effect of Qing Fei Hua Xian Decotion (QFHXD), a traditional Chinese medicine formula applied for treating PF in COVID-19 survivors, is unclear. This study aimed to uncover the mechanisms related to the anti-PF effect of QFHXD through analysis of network pharmacology and experimental verification.
Methods
The candidate chemical compounds of QFHXD and its putative targets for treating PF were achieved from public databases, thereby we established the corresponding “herb- compound -target” network of QFHXD. Moreover, The protein–protein interaction (PPI) network of potential targets was also constructed to screen the core targets. Furthermore, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to predict targets, and pathways, then validated by in vivo experiments.
Results
A total of 188 active compounds in QFHXD and 50 target genes were identified from databases. The key therapeutic targets of QFHXD, such as PI3K/Akt, IL-6, TNF, IL-1β, STAT3, MMP-9, and TGF-β1 were identified by KEGG and GO analysis. Anti-PF effects of QFHXD (in a dose-dependent manner) and prednisone were confirmed by HE, Masson staining, and Sirius red staining as well as in vivo Micro-CT and immunohistochemical analysis in a rat model of bleomycin-induced PF. Besides, QFXHD remarkably inhibits the activity of PI3K/Akt/NF-κB and TGF‑β1/Smad2/3.
Conclusion
QFXHD significantly attenuated bleomycin-induced PF via inhibiting inflammation and EMT. PI3K/Akt/NF-κB and TGF‑β1/Smad2/3 pathways might be the potential therapeutic effects of QFHXD for treating PF.
New technology of multi-directional loading rotary extrusion
Authors: Zhang Zhimin , Xue Yong , Zhang Xing , Dong Beibei , Yan Lin , Chen Zhe 
Published: June 2022

Abstract
Aiming at the demand of precise forming of large-scale high-performance lightweight components with inner ring reinforcement, a new multi-directional loading rotary extrusion forming technology is developed, which matches the linear motion with the rotary motion and actively increases the strong shear force. Its principle is that while the blank is axially extruded and loaded, the radial force and rotating torque are increased. Through the synergistic action of axial, radial and rotating motion, the orderly flow of metal is controlled and the cumulative severe plastic deformation (SPD) of "uplift-trowel" micro area is generated, so as to realize the uniform strengthening and toughening of materials. At the same time, through the continuous deformation of punch "ellipse-circle", high reinforcement is grown on the cylinder wall to achieve the high-quality forming of cylindrical parts or inner ring reinforcement components. This technology enriches the plastic forming theory and widens the application field of plastic forming. In addition, the formed large-scale high-performance inner ring stiffened magnesium (Mg) components have been successfully verified in aerospace equipment, solves the problems of integral forming and severe deformation strengthening and toughening of large-scale inner ring stiffened components, and has a good prospect of popularization and application.
A ten-gene retinal pigment epithelium (RPE)/choroid complex diagnosis signature for age-related macular degeneration
Authors: Li He-Yan , Dong Li , Zhou Wen-Da , Wu Hao-Tian , Li Yi-Fan , Zhang Rui-Heng , Wei Wen Bin 
Published: June 2022

Abstract
Purpose
Age-related macular degeneration (AMD) is a multifactorial disease in the elderly with a prominent genetic basis. This study aimed to apply machine learning method to develop a novel diagnostic model for AMD based on gene biomarkers in RPE/choroid complex, which may be potential therapeutic targets.
Methods
We collected RPE/choroid tissue gene expression profiles of AMD and normal patients from the Gene Expression Omnibus (GEO) database as training and validation cohorts. After differential expression analysis and the selection of gene biomarkers by random forest algorithms, selected genes were applied to the least absolute shrinkage and selection operator (LASSO) logistic regression to construct a diagnostic model in the training cohort. The diagnostic ability of the model was further tested in the validation cohort. Gene set enrichment analysis (GSEA) and immune cell assessment were also conducted for further analyses.
Results
A noval diagnostic model based on ten genes (BMPR2, CNOT3, CRLF1, FXYD6, HRASLS5, KRTDAP, NUDT16L1, PI16, PLAGL1, SART1) was constructed in the training cohort. The AUC in the training cohort reached 0.908 (95% CI: 0.823–0.975), while it remained 0.809 (95% CI: 0.522–0.889) in the validation cohort. According to the GSEA analysis, glutathione metabolism and phototransduction pathway are the two shared enriched pathways in the training and validation cohorts. Functional enrichment analysis and immune cell evaluation demonstrated that AMD was significantly correlated with both adaptive and innate immune cells, and the levels of neutrophil in the high-risk group were significantly higher than that in the low-risk group in both training and validation datasets
Conclusion
We identified and validated a novel ten-gene-based diagnostic model with high accuracy for AMD. The current study provided a promising tool to be used as a precise and cost-effective non-invasive test in clinical practice.
Business Constraints and Firm Exit in Developing Countries: Evidence from Nigeria
Authors: ORJIAKOR EBUKA 
Published: June 2022

Abstract
This study examines the impact of business constraints on firm exit using harmonized dataset of panel firms from the World Bank Enterprise Survey in Nigeria. It applies a confirmatory methodology – factor analysis to aggregate 15 firm-level indicators of business constraints into an index scale and estimates firm likelihood of exit from the market using binary probit model. Findings suggest that firm exit in Nigeria is not determined by current level of constraints in the business environment; however, additional level of constraints will result to more firms exiting the market. This finding holds true in the entire model specifications; that is, with and without accounting for firms’ differences or demographics, the squared business constraint index has a significant positive association with firm exit. By implication, addressing constraints in the business environment can improve business survival prospect and in return contribute to national development. Therefore, it is imperative that policymakers in developing countries like Nigeria should gear more efforts towards improving business environment in their economies through carefully designed policies that can foster private sector development.
Identifying Soil Erosion Hotspot Area Using GIS and MCDA Techniques, Case Study of Dengora and Meno Watersheds in Belesa Woredas, Amhara Region
Authors: Munye Kefale , Sintayehu Gashaw , Belayneh Alemshet , Mulat Chalachew 
Published: June 2022

Abstract
In Ethiopia soil erosion and land degradation has become a key issue, because of roughed and steep slope topography that soil erosion become accelerating. The main objective of this study was to identify soil erosion hotspot areas in Dengora and Meno watersheds using Revised Universal Soil Loss Equation (RUSLE) and Multi Criteria Decision Analysis (MCDA) techniques. To achieve such objective RUSLE model was used to estimate potential soil losses by utilizing information on Soil, land use/cover, topography, and climatic data. MCDA technique considered land use, soil type, topographic wetness index, stream power index and potential location of gullies. The factors were weighted using pair-wise comparison matrix and weights were combined using Weighted Overlay. Based on RUSLE model, the average annual soil loss of Dengora and Meno watersheds were reaches up to 223.97and 256.09-ton ha-1yr-1 respectively. In Dengora watershed 70.4%, 18.7%, 10.74% and 0.14% and Meno watershed 76%, 16.54%, 7.3% and 0.14% of the total watershed area slightly, moderately, highly and very highly sensitive to soil erosion respectively. On the other hand, GIS based MCDA technique reveals that in Dengora watershed 9.7%, 64.5%, 18% and 7.8% and Meno watershed 6.1%, 71.3%, 23.23% and 0.375% of the total watershed area was highly, moderately, slightly and currently not sensitive to soil erosion respectively. Based on validation, field level observation, MCDA model prediction was more accurate than RUSLE. Both of the watersheds were at moderate risk. Bottomlands of the watersheds under were highly sensitive areas for erosion. Therefore, immediate attention for soil and water conservation practices.
The “Y”-shaped Denonvilliers’ fascia and its adjacent relationship with the urogenital fascia based on the male cadaveric anatomical study
Authors: Yi Li , Ya-Min Zhao , Yan-Bing Ma , Jin-Song Zhou , Cong Tong , Li-Kun Yan 
Published: June 2022

Abstract
Background
The controversies about the anatomical structure of the Denonvilliers’ fascia and the relationship with surrounding fasciae have sparked a heated discussion, especially about whether it contains multilayers. This study aimed to expound on the anatomical structure of the Denonvilliers’ fascia and its correlation with the peritoneum from the sagittal position and clarify the complex fasciae relationship.
Methods
Our study was performed on 20 adult male pelvic specimens fixed in formalin, including 2 entire pelvic specimens and 18 semi-pelvic specimens. By dissecting the local adjacent organs and fasciae, the Denonvilliers’ fascia was observed and removed for histological examination.
Results
Almost the Denonvilliers’ fascia was a single layer and tough fascia. In the sagittal position, the peritoneum constituting peritoneum reflection and the Denonvilliers’ fascia showed a “Y” shape. The Denonvilliers’ fascia was originated from the peritoneum reflection, continually ran on the ventral side of the seminal vesicles and prostate, terminated at the perineal body, and gradually both sides thinned to fuse with the urogenital fascia (UGF). In addition, the histology preliminarily manifested that the basal cell layers of the peritoneum and that of the Denonvilliers’ fascia were continuous, and they also showed a “Y” shape. Also, the basal cells of the two peritonea extended to the Denonvilliers’ fascia, showing a fused double-layer structure. Some tiny blood vessels or a network of them extended from the peritoneum to the Denonvillier’ fascia.
Conclusion
As the extension of the peritoneum in the pelvic floor, the Denonvilliers’ fascia is a “Y”-shaped monolayer layer fascia in the sagittal position. Our study provides new support for the peritoneum fusion theory of it. Understanding the anatomical characteristics of the Denonvilliers’ fascia and its relationship with the UGF is a guiding significance for youth colorectal surgeons to conduct rectal cancer surgery.
Synthesis, structural and theoretical studies on new morpholino acetamide ligand and rare earth metal complexes and corrosion Inhibition Effect
Authors: Ahmed Yasmin mamdoh hussien , Ashmawy Ashraf M. , Abbas Ashraf.A. , Mohamed Gehad G. 
Published: June 2022

Abstract
A series of new complexes of the general formula [Ln(H2L)Cl2]Cly.4H2O (Ln = Er and Ta) and [Yb(H2L)(H2O)2]Cl3, H2L = N,N'-((ethane-1,2-diylbis(sulfanediyl))bis(2,1-phenylene))bis(2-morpholinoacetamide) are synthesized and characterized. The physical properties of these complexes are investigated including melting point, color, and percent yield. At room temperature, complexes exhibit similar solution absorption spectra in the UV–Vis region. As shown by thermal analysis, the complexes lose water molecules first, then anions, and finally ligand molecules, leaving a metal oxide residue. The complexes were resulted from monomolar coordination of neutral tetradentate ligand through the amide nitrogen and sulfur, with octahedral geometries. Ligand and its Ta(V) complex are structurally characterized by X-ray powder diffraction. Their optical band gaps demonstrated the ligand and complexes have semiconducting property. Aluminum alloys (AlSi) corrosion inhibition was investigated in 1 M HCl using potentiodynamic polarization (PP) and electrochemical impedance spectroscopy (EIS). The inhibition efficiency was 96.36% at 500 ppm inhibitor in 1 M HCl.. Multiple bonding patterns were discovered in molecular docking simulations of the drugs against SARS-CoV-2 (6Y84) and E. coli DNA gyrase (5MMN). The binding energy of complexes revealed active compounds capable of inhibiting SARS-CoV-2 and E. coli DNA gyrase. The antioxidant activity of the ligand and its Ln-complexes was investigated, yielding some surprising results.Finally, it is worthy to note that, the biochemical outcomes revealed that these complexes may be promising antibacterial agents.
Clinical features and treatment strategies of Helicobacter cinaedi infections in emergency departments: a descriptive study
Authors: Izuta Kento , Matsuoka Yoshinori , Hasuike Toshikazu , Hijikata Yasukazu , Kuwahara Yusuke , Mizu Daisuke , Ariyoshi Koichi 
Published: June 2022

Abstract
Background: Helicobacter cinaedi, a Gram-negative spiral bacterium, is a rare cause of bacteraemia in humans. It is increasingly reported in both immunocompetent and immunocompromised patients. Nevertheless, little is known about H. cinaedi infections in the emergency departments (ED). Our study aimed to describe the clinical features of H. cinaedi infections in the ED.Methods: We conducted the descriptive study at the ED of Kobe City General Hospital between November 2011 and December 2020. We included all ED patients with H. cinaedi infections, and all H. cinaedi were isolated in blood cultures. Following bacterial culture, clinical specimens with gram-negative spiral bacilli underwent polymerase chain reactions to confirm H. cineadi infection. We retrospectively obtained the patient data from the electronic medical records and described patient characteristics, clinical course, and management of H. cinaedi bacteraemia. Results: During the study period, 22 patients in the ED were diagnosed with H. cinaedi bacteraemia. The youngest patient was 32 years old, and the oldest was 91 (median: 65.5 [interquartile range, 49.3 to 63.3] years). Diabetes mellitus was the most common comorbidity (7/22, 31.8%), followed by haematological malignancy and solid tumour (5/22, 22.7%), recent chemotherapy (4/22, 18.2%), while 3/22 (13.6%) patients had no comorbidities. In our cohort, complicated cases were identified: vertebral osteomyelitis (three cases), infected aortic aneurysm (two cases), and infected cyst (renal cyst, and pancreatic cyst with concomitant empyema) (two cases). Tetracycline (minocycline) was most commonly prescribed; administered intravenously in 5/15 (33.3%) and orally in 9/20 (45.0%) patients. Only one (4.5%) patient required surgical interventions. None died in the hospital.Conclusions: We reported 22 cases of H. cinaedi bacteraemia in the ED, most of which were successfully treated with antibiotics, primarily tetracycline. This report gives an important insight into the presentation and management of such infections. We suggest that future studies are warranted to evaluate the optimal choice and route of antibiotic treatment in H. cinaedi infections.
Insights into the primitives and sequence of 7YSZ TBCs deposition mechanism
Authors: Huang Rongchen , Yang Wenchao , Fan Xiujuan , Mao Jie , Liu Min , Zhan Yongzhong , Zhang Xiaofeng 
Published: June 2022

Abstract
The deposition mechanism of 7YSZ (7 wt% Y2O3-ZrO2) thermal barrier coatings (TBCs) prepared by plasma spray–physical vapor deposition (PS-PVD) and electron beam–physical vapor deposition (EB-PVD) were investigated in detail. The results show that the deposition mechanism of 7YSZ TBCs can be divided into two stages, including primitives and sequences. These coating primitives (grains) were deposited using atomic vapor. The PS-PVD primitives were short rod–shaped, while the EB-PVD primitives were needle-shaped. The PS-PVD coating primitives were ordered in the interior but disordered on the surface due to quenching under the influence of the plasma jet. However, the EB-PVD coating primitives were ordered from the interior to the surface. The feather-like columnar structure in PS-PVD was sequenced due to the vapor-liquid-solid mixed-phases deposition. In contrast, the needle-like columnar structure in EB-PVD was sequenced because of the single-vapor phase deposition.
Sustainable nano-interventions to enhance crop yield, anthocyanin content, and marketability of onion (Allium cepa)
Authors: Jangir Himanshi , Kaler Brijesh , Srivast Gaurav , Das Mainak 
Published: June 2022

Abstract
Our reliance on synthetic agrochemicals has detrimental effects on the environment and living species. To restore the ecological balance, we need to explore strategies to minimize agrochemicals’ use without compromising agricultural production. Nano-formulations help lower dosages of agrochemicals, leading to the emergence of nano-agriculture. Judicious use of nanomaterials for enhancing plant metabolic performance and yield remains a challenge. Here, we have achieved the same by root treatment of the onion crop with nanopyrite (FeS2) and further organic goat manure fertilizer application. We report an increase in yield through root treatment with nano-pyrite plus soil application of goat dropping (Test) as compared to the use of goat dropping alone (Control). The total biomass of the test sample was 4.75 kg (n=86), while control samples weighed 3.5 kg (n=86). The total bulb weight for the control and test was 2.6 kg and 3.6 kg, respectively. We observed a yield-boosting effect of root treatment with nanopyrite in onion. There is a significant increase in the anthocyanin content in test bulbs and flavonol content in test leaves. We have integrated two types of farming systems: organic and nano-agriculture to bolster the metabolic fitness of the onion (Allium cepa) and achieve sustainable food production.
Hypoxia score for predicting prognosis and tumor response to immunotherapy in bladder urothelial carcinoma
Authors: Li Yinghao , Zhang Yanlong , Chen Chao , Zhao Sicong , Zhang Xinwei , Zhang Jian , Yan Yong 
Published: June 2022

Abstract
Numerous types of cancers are characterized by hypoxia. However, the effects of hypoxia on cancer immunology, tumor growth, and therapeutic responses in bladder urothelial carcinoma (BLCA) remain unknown. In this study, we examined the biological characteristics of hypoxia and hypoxia-related gene clusters in BLCA. Clustering analysis was performed to identify the subtypes of BLCA based on hypoxia biomarker genes. Principal component analysis and hypoxia-related genes were used to calculate a hypoxia score for BLCA. The level of infiltration of immune and stromal cells in the BLCA immune microenvironment was quantified using CIBERSORT and ESTIMATE. A hazard ratio was determined for the hypoxia score through meta-analysis of three separate BLCA cohorts. The association between the hypoxia score and the tumor microenvironment was determined using pan-cancer analysis. A tumor immunotherapy cohort and the Genomics of Drug Sensitivity in Cancer database were used to examine the predictive value of the hypoxia score in relation to various treatment options. We evaluated 1,449 BLCA samples, 5 cell lines, and 9,858 samples from 32 different tumor types. In the BLCA cohort, three hypoxia and two hypoxia-related gene clusters with different hypoxia levels in the tumor microenvironment were found. The final hypoxia scoring system considered 77 hypoxia-related genes. A low hypoxia score was associated with high KDM6A, FGFR3, and OBSCN mutation rates; better prognosis; and a strong response to immunotherapy. The understanding of tumor invasion and metastasis can be improved by analyzing the hypoxia patterns in different tumor microenvironments. The hypoxia scoring system may be useful for determining patient prognosis and personalized immune treatment plans.
Valorization of Residual Biomass From Forest Harvesting Through Its Pelletization
Authors: Lúcio Danielle de Moraes , Hillig Everton , Neto Raul de Abreu , Lopes Eduardo da Silva , Silva Dimas Agostinho da 
Published: June 2022

Abstract
Residual biomass from forest harvesting consists of a mixture of wood, bark, leaves and needles that are often used to generate energy. In addition, this biomass can remain stored in the field for up to six months, which can affect its physical and energy properties. The pelleting process can be an alternative to improve the energy properties, transport and storage of this biofuel, allowing companies to better manage these resources. Thus, the effect of storage time in the field of the harvesting residual biomass of pine and eucalyptus on the properties of its pellets were evaluated. The results show that there is little influence of the storage time on the energy properties and there is a tendency to increase the physical and mechanical properties of the pellets produced from biomass with longer storage time in the field. We show that benefits can be obtained by producing pellets with forest harvest residues, even if they contain a non-woody fraction and regardless of the storage time in the field.