bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of activity recognition models for mechanical fuel treatments using consumer-grade GNSS-RF devices and lidar
Authors: Becker Ryer M , Keefe Robert F 
Published: January 2022

Abstract
Mobile technologies are rapidly advancing the field of forest operations and providing opportunities to quantify management tasks in new ways through increased digitalization. For instance, devices equipped with global navigation satellite system and radio frequency transmission (GNSS-RF) enable real-time data collection and sharing of positional data in remote, off-the-grid environments where cellular and internet availability are otherwise inaccessible. In this study, consumer-grade GNSS-RF data were evaluated to determine their effectiveness in developing activity recognition models for excavator-based mastication operations. The ability to automate the classification of cycle elements for operations is valuable for quickly and efficiently quantifying production rates for research and industry applications. The GNSS-RF-based activity recognition model developed successfully classified productive elements versus delay with over 95 per cent accuracy. Individual cycle elements were classified with an overall model accuracy of 73.6 per cent, with individual element classification accuracy ranging from 51.3 per cent for walk/reposition to 95.6 per cent for mastication elements. Reineke’s stand density index, basal area (m2 ha−1) of treated areas and the duration of cycle elements impacted the classification accuracy of the activity recognition model. Impacts of forest stand characteristics on the production rate of mastication treatments were also assessed. Production rates (ha·hr−1) for mastication treatments were affected by the basal area of treated areas. However, the degree to which this would impact operations in practice is minimal. Determining the proper application and capabilities of mobile technologies and remote sensing for quantifying forest operations is valuable in continuing the innovation and advancement of forest digitalization.
A conditional glutamatergic synaptic vesicle marker forDrosophila
Authors: Certel Sarah J , Ruchti Evelyne , McCabe Brian D , Stowers R Steven 
Published: January 2022

AbstractGlutamate is a principal neurotransmitter used extensively by the nervous systems of all vertebrate and invertebrate animals. It is primarily an excitatory neurotransmitter that has been implicated in nervous system development, as well as a myriad of brain functions from the simple transmission of information between neurons to more complex aspects of nervous system function including synaptic plasticity, learning, and memory. Identification of glutamatergic neurons and their sites of glutamate release are thus essential for understanding the mechanisms of neural circuit function and how information is processed to generate behavior. Here, we describe and characterize smFLAG-vGlut, a conditional marker of glutamatergic synaptic vesicles for the Drosophila model system. smFLAG-vGlut is validated for functionality, conditional expression, and specificity for glutamatergic neurons and synaptic vesicles. The utility of smFLAG-vGlut is demonstrated by glutamatergic neurotransmitter phenotyping of 26 different central complex neuron types of which nine were established to be glutamatergic. This illumination of glutamate neurotransmitter usage will enhance the modeling of central complex neural circuitry and thereby our understanding of information processing by this region of the fly brain. The use of smFLAG for glutamatergic neurotransmitter phenotyping and identification of glutamate release sites can be extended to any Drosophila neuron(s) represented by a binary transcription system driver.
Lung Function Decline in Relation to COVID-19 in the General Population: A Matched Cohort Study With Prepandemic Assessment of Lung Function
Authors: Iversen Katrine K , Afzal Shoaib , Ahlström Magnus G , Nordestgaard Børge G , Schneider Uffe V , Nielsen Lene , Kofoed Klaus , Benfield Thomas , Ronit Andreas 
Published: January 2022

Abstract

Background
To quantify the potential decline in dynamic lung volumes following coronavirus disease 2019 (COVID-19) in the general population.

Methods
A prospective matched cohort study of adult Copenhagen General Population Study (CGPS) participants with a prepandemic spirometry available. CGPS individuals with positive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) polymerase chain reaction (PCR) test performed repeat spirometry, a questionnaire regarding respiratory symptoms, and diffusing capacity test for carbon monoxide. A matched uninfected CGPS control sample was used, and simple regression and linear mixed effect models were computed to study lung function decline.

Results
A total of 606 individuals were included; 92/107 (85.9%) with positive SARS-CoV-2 PCR test experienced coronavirus disease 2019 (COVID-19) symptoms and 12 (11.2%) were hospitalized. Spirometry was performed at median 5.6 months (interquartile range, 3.9–12.8) after positive SARS-CoV-2 PCR test. COVID-19 was associated with adjusted 7.3 mL (95% confidence interval [CI], .3–14.3) and 22.6 mL (95% CI, 13.1–32.0) steeper decline in annual forced expiratory volume in first second (FEV1) and FVC or total 113.8 and 301.3 mL lower FEV1 and FVC from baseline to follow-up. Results were robust in analyses restricted to individuals not requiring hospitalization.

Conclusions
COVID-19–related declines of dynamic lung volume in the general population not requiring hospitalization were small but measurable.
Five-Year Retrospective Analysis of a Vented Mobility Algorithm in the Burn ICU
Authors: O’Neil Audrey M , Rush Cassandra , Griffard Laura , Roggy David , Boyd Allison , Hartman Brett C 
Published: January 2022

Abstract
Early mobilization with mechanically ventilated patients has received significant attention within recent literature; however, limited research has focused specifically on the burn population. The purpose of this single-center, retrospective analysis was to review the use of a burn critical care mobility algorithm, to determine the safety and feasibility of a burn vented mobility program, share limitations preventing mobility progression at our facility, and discuss unique challenges to vented mobility with intubated burn patients. A retrospective review was completed for all intubated burn center admissions between January 2015 and December 2019. Burn therapy notes were then reviewed for data collection, during the intubation period, using stages of the mobility algorithm. In the 5-year period following initial implementation, the vented mobility algorithm was utilized on 127 patients with an average TBSA of 22.8%. No adverse events occurred. Stage 1 (range of motion) was completed with 100% of patients (n = 127). Chair mode of bed, stage 2a, was utilized in 39.4% (n = 50) of patients, while 15.8% (n = 20) of patients were dependently transferred to the cardiac chair in stage 2b. Stage 3 (sitting on the edge of the bed) was completed with 25% (n = 32) of patients, with 11% (n = 14) progressing to stage 5 (standing), and 3.9% (n = 5) actively transferring to a chair. In the 5 years, only 4.7% (n = 6) reached stage 6 (ambulation). The most common treatment limitations were medical complications (33%) and line placement (21%). Early mobilization during mechanical ventilation is safe and feasible within the burn population, despite challenges, including airway stability, sedation, and line limitations.
Liquid biopsy detection of genomic alterations in pediatric brain tumors from cell-free DNA in peripheral blood, CSF, and urine
Authors: Pagès Mélanie , Rotem Denisse , Gydush Gregory , Reed Sarah , Rhoades Justin , Ha Gavin , Lo Christopher , Fleharty Mark , Duran Madeleine , Jones Robert , Becker Sarah , Haller Michaela , Sinai Claire E , Goumnerova Liliana , Golub Todd R , Love J Christopher , Ligon Keith L , Wright Karen D , Adalsteinsson Viktor A , Beroukhim Rameen , Bandopadhayay Pratiti 
Published: January 2022

AbstractBackgroundThe ability to identify genetic alterations in cancers is essential for precision medicine; however, surgical approaches to obtain brain tumor tissue are invasive. Profiling circulating tumor DNA (ctDNA) in liquid biopsies has emerged as a promising approach to avoid invasive procedures. Here, we systematically evaluated the feasibility of profiling pediatric brain tumors using ctDNA obtained from plasma, cerebrospinal fluid (CSF), and urine.MethodsWe prospectively collected 564 specimens (257 blood, 240 urine, and 67 CSF samples) from 258 patients across all histopathologies. We performed ultra-low-pass whole-genome sequencing (ULP-WGS) to assess copy number variations and estimate tumor fraction and developed a pediatric CNS tumor hybrid capture panel for deep sequencing of specific mutations and fusions.ResultsULP-WGS detected copy number alterations in 9/46 (20%) CSF, 3/230 (1.3%) plasma, and 0/153 urine samples. Sequencing detected alterations in 3/10 (30%) CSF, 2/74 (2.7%) plasma, and 0/2 urine samples. The only positive results were in high-grade tumors. However, most samples had insufficient somatic mutations (median 1, range 0-39) discoverable by the sequencing panel to provide sufficient power to detect tumor fractions of greater than 0.1%.ConclusionsChildren with brain tumors harbor very low levels of ctDNA in blood, CSF, and urine, with CSF having the most DNA detectable. Molecular profiling is feasible in a small subset of high-grade tumors. The level of clonal aberrations per genome is low in most of the tumors, posing a challenge for detection using whole-genome or even targeted sequencing methods. Substantial challenges therefore remain to genetically characterize pediatric brain tumors from liquid biopsies.
In-plane and vertical heterostructures from 1T′/2H transition-metal dichalcogenides
Authors: Ma Yang , Xu Shiyu , Wei Juntian , Zhou Bin , Gong Yongji 
Published: January 2022

Abstract
An avalanche of research has been carried out on two-dimensional (2D) transition metal dichalcogenides (TMDs) due to their potential applications in advanced electronics and flexible devices. To take full use of the emerging 2D TMDs materials, their in-plane/vertical heterostructures have been explored, enabling effective tuning of their physical and chemical properties. However, structural differences between the various phases impede the formation of functional heterostructures. Therefore, robust synthesis strategies for heterostructures with different phases have been explored in this study. A chemical vapor deposition process has been proposed in which the key parameters like reaction sources and deposition sites have been carefully adjusted, trying to achieve simultaneous synthesis of 1T′/2H in-plane and vertical heterostructures. Consequently, 2D in-plane RexMo1−xS2/MoS2 and vertical ReS2/MoS2 heterostructures have been produced in different regions at the same time. Atomic-resolution Z-contrast images reveal the detailed atomic structure of the 1T′/2H interfaces. The lateral interface is found to contain Mo atoms with only 5-fold coordination with S due to the phase mismatch. This work demonstrates a route to exploit heterostructures of different phases and opens the possibility to build more complicated 2D heterostructures using CVD.
Silicon Reduces Aluminum-Induced Suberization by Inhibiting the Uptake and Transport of Aluminum in Rice Roots and Consequently Promotes Root Growth
Authors: Xiao Zhuoxi , Ye Mujun , Gao Zixiang , Jiang Yishun , Zhang Xinyuan , Nikolic Nina , Liang Yongchao 
Published: January 2022

AbstractSilicon (Si) can alleviate aluminum (Al) toxicity in rice (Oryza sativa L.), but the mechanisms underlying this beneficial effect have not been elucidated, especially under long-term Al stress. Here, the effects of Al and Si on the suberization and development of rice roots were investigated. The results show that, as the Al exposure time increased, the roots accumulated more Al, and Al enhanced the deposition of suberin in roots, both of which ultimately inhibited root growth and nutrient absorption. However, Si restricted the apoplastic and symplastic pathways of Al in roots by inhibiting the uptake and transport of Al, thereby reducing the accumulation of Al in roots. Meanwhile, the Si-induced drop in Al concentration reduced the suberization of roots caused by Al through down-regulating the expression of genes related to suberin synthesis and then promoted the development of roots (such as longer and more adventitious roots and lateral roots). Moreover, Si also increased nutrient uptake by Al-stressed roots and thence promoted the growth of rice. Overall, these results indicate that Si reduced Al-induced suberization of roots by inhibiting the uptake and transport of Al in roots, thereby amending root growth and ultimately alleviating Al stress in rice. Our study further clarified the toxicity mechanism of Al in rice and the role of Si in reducing Al content and restoring root development under Al stress.
Exotic species of rotifers in Mexico
Authors: Nandini S , Sarma S S S , Wallace R L 
Published: January 2022

Abstract
Mexico is a megadiverse country, with 10% of all known species found within its borders. The CONABIO catalogue registers rotifers as one of the best-studied groups of animals in Mexico, with the number of species recorded representing 18% of the total global rotifer fauna. However, this registry does not record a single exotic species of Rotifera in Mexico. Here, we confirm the presence of six species of exotic rotifers in Mexican inland waters, highlighting the case of Kellicottia bostoniensis, recorded in Mexico since the 1990’s, but never as an exotic species.
Effectiveness of Specific Exercise for Deep Cervical Muscles in Nonspecific Neck Pain: A Systematic Review and Meta-Analysis
Authors: Garzonio Stefano , Arbasetti Chiara , Geri Tommaso , Testa Marco , Carta Giacomo 
Published: January 2022

Objective

Objective
The purpose of this review, focused on people with nonspecific neck pain (NSNP), was to assess the effectiveness of specific exercises that recruit the deep cervical muscles compared with other types of exercises or interventions and minimal or no treatment.

Methods
This systematic review with meta-analysis screened 5 databases (MEDLINE, Embase, CINAHL, Physiotherapy Evidence Database, and The Cochrane Library). Randomized controlled trials investigating the effectiveness of this type of exercise on the intensity of pain were included. Data for each included trial were extracted by 2 independent reviewers. The Cochrane Risk of Bias 2.0 tool was adopted to assess the internal validity of the included trials. The overall quality of evidence was layered with the Grading of Recommendations, Assessment, Development and Evaluations system.

Results
The review identified a total of 2397 records. Sixteen articles were included in the qualitative synthesis, and 9 studies were included in the meta-analysis. The pooled results found moderate- to very low-quality evidence that deep cervical muscle exercise protocols are not superior to other types of active exercises in reducing the intensity of pain in people with NSNP. Studies not included in the meta-analysis suggest that specific exercises induce better effects that are superior to those of nontreatment with clinically relevant results.

Conclusion
Clinicians can share this information with their patients through shared decision-making to determine a more tailored approach to adopt. Future studies with high methodological quality are necessary to reach firm conclusions.

Impact
There has been no consensus on exercise type and dosage for the management of NSNP. This study shows that exercises are a useful tool and that the effect of an exercise program that recruits deep cervical muscles seems to be comparable with the effect of other types of active exercises on pain intensity reduction.

Lay summary
If you have neck pain, exercises designed by a physical therapist can be a useful treatment tool. An exercise program that targets specific deep cervical muscles may be just as good as other types of active exercises to reduce the intensity of pain intensity; you and your therapist can work together to develop a program.
Prx1
+ and Hic1+ Mesenchymal Progenitors Are Present Within the Epidural Fat and Dura Mater and Participate in Dural Injury Repair
Authors: Shah Sophia , Mudigonda Sathvika , Underhill Tully Michael , Salo Paul T , Mitha Alim P , Krawetz Roman J 
Published: January 2022

Abstract
Epidural fat is commonly discarded during spine surgery to increase the operational field. However, mesenchymal progenitor cells (MPCs) have now been identified in human epidural fat and within the murine dura mater. This led us to believe that epidural fat may regulate homeostasis and regeneration in the vertebral microenvironment. Using two MPC lineage tracing reporter mice (Prx1 and Hic1), not only have we found that epidural fat MPCs become incorporated in the dura mater over the course of normal skeletal maturation, but have also identified these cells as an endogenous source of repair and regeneration post-dural injury. Moreover, our results reveal a partial overlap between Prx1+ and Hic1+ populations, indicating a potential hierarchical relationship between the two MPC populations. This study effectively challenges the notion of epidural fat as an expendable tissue and mandates further research into its biological function and relevance.
Alterations in Hematopoietic and Mesenchymal Stromal Cell Components of the Osteopetrotic Bone Marrow Niche
Authors: Zeytin Inci Cevher , Alkan Berna , Ozdemir Cansu , Cetinkaya Duygu Uckan , Okur Fatma Visal 
Published: January 2022

AbstractOsteopetrosis is a rare inherited disease characterized by impaired osteoclast activity causing defective bone resorption and bone marrow aplasia. It is fatal in early childhood unless hematopoietic stem cell transplantation is performed. But, the transplant course is complicated with engraftment failure. Recently, osteoclasts have been described as the potential regulators of hematopoietic stem cell (HSC) niche. Here we investigated the alterations in the HSC and mesenchymal stromal cell (MSC) components of osteopetrotic niche and their interactions to mimic the stem cell dynamics/trafficking in the BM niche after HSC transplantation. Induced pluripotent stem cells were generated from peripheral blood mononuclear cells of patients with osteopetrosis carrying TCIRG1 mutation. iPSC lines were differentiated into hematopoietic and myeloid progenitors, then into osteoclasts using a step-wise protocol. We first demonstrated a shift toward monocyte-macrophages lineage regarding hematopoietic differentiation potential of osteopetrotic iPSC-derived hematopoietic progenitors (HPCs) and phenotypically normal and functionally defective osteoclast formation. The expression of the genes involved in HSC homing and maintenance (Sdf-1, Jagged-1, Kit-L, and Opn) in osteopetrotic MSCs recovered significantly after coculture with healthy HPCs. Similarly, the restoration of phenotype, impaired differentiation, and migratory potential of osteopetrotic iHPCs were observed upon interaction with healthy MSCs. Our results establish significant alterations in both MSC and HPC compartments of the osteopetrotic niche, and support the impact of functionally impaired osteoclasts in defective niche formation.
Analysis of Outer Membrane Vesicles Indicates That Glycerophospholipid Metabolism Contributes to Early Symbiosis Between Sinorhizobium fredii HH103 and Soybean
Authors: Li Dongzhi , Li Ziqi , Wu Jing , Tang Zhide , Xie Fuli , Chen Dasong , Lin Hui , Li Youguo 
Published: January 2022

Gram-negative bacteria can produce outer membrane vesicles (OMVs), and most functional studies of OMVs have been focused on mammalian-bacterial interactions. However, research on the OMVs of rhizobia is still limited. In this work, we isolated and purified OMVs from Sinorhizobium fredii HH103 under free-living conditions that were set as control (C-OMVs) and symbiosis-mimicking conditions that were induced by genistein (G-OMVs). The soybean roots treated with G-OMVs displayed significant deformation of root hairs. G-OMVs significantly induced the expression of nodulation genes related to early symbiosis, while they inhibited that of the defense genes of soybean. Proteomics analysis identified a total of 93 differential proteins between C-OMVs and G-OMVs, which are mainly associated with ribosome synthesis, flagellar assembly, two-component system, ABC transporters, oxidative phosphorylation, nitrogen metabolism, quorum sensing, glycerophospholipid metabolism, and peptidoglycan biosynthesis. A total of 45 differential lipids were identified through lipidomics analysis. Correlation analysis of OMV proteome and lipidome data revealed that glycerophospholipid metabolism is the enriched Kyoto Encyclopedia of Genes and Genomes metabolic pathway, and the expression of phosphatidylserine decarboxylase was significantly up-regulated in G-OMVs. The changes in three lipids related to symbiosis in the glycerophospholipid metabolism pathway were verified by enzyme-linked immunosorbent assay. Our results indicate that glycerophospholipid metabolism contributes to rhizobia-soybean symbiosis via OMVs. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Remediation in Practice: A Polarity to be Managed
Authors: Bourgeois-Law Gisèle , Varpio Lara , Teunissen Pim , Regehr Glenn 
Published: January 2022

Abstract:
Originally developed in the business literature, a polarity is a concept where 2 distinctive and opposing characteristics (poles), each presenting advantages and disadvantages or opportunities and pitfalls, must both be taken into account to ensure effective management of a challenging problem. Managing a polarity is a thorny endeavor because it entails striving to maximize the benefits of both poles while simultaneously minimizing or controlling the downsides of each. Previous investigations into stakeholder conceptualizations of remediation led us to suggest that remediation is framed in stakeholders' minds simultaneously as an educational endeavor (ie, the remediatee needs educational support to regain full competence) and a regulatory act (ie, the revocation of the individual’s professional right to self-regulate their practice and learning). In this article, we argue that viewing remediation for practicing physicians as a polarity to be managed offers a framework that can further the conversation about how to address some of remediation’s challenges.
Modelling of hemodynamics in bifurcation lesions of coronary arteries before and after myocardial revascularization
Authors: Starodumov Ilya O. , Sokolov Sergey Yu. , Alexandrov Dmitri V. , Zubarev Andrey Yu. , Bessonov Ivan S. , Chestukhin Vasily V. , Blyakhman Felix A. 
Published: January 2022

Modelling of patient-specific hemodynamics for a clinical case of severe coronary artery disease with the bifurcation stenosis was carried out with allowance for standard angiographic data obtained before and after successfully performed myocardial revascularization by stenting of two arteries. Based on a non-Newtonian fluid model and an original algorithm for fluid dynamics computation operated with a limited amount of initial data, key characteristics of blood flow were determined to analyse the features of coronary disease and the consequences of its treatment. The results of hemodynamic modelling near bifurcation sites are presented with an emphasis on physical, physiological and clinical phenomena to demonstrate the feasibility of the proposed approach. The main limitations and ways to minimize them are the subjects of discussion as well.
This article is part of the theme issue ‘Transport phenomena in complex systems (part 2)’.