bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Traits Loci and candidate gene mapping for Quinones and Phenolic Biosynthesis in Nigella sativa L. using pseudo-chromosome Assembly Arusa Aftab, Zubaida Yousaf, Zill-e-Huma Aftab, Nadia Riaz, Bushra Shamsheer
Authors: Rashid Madiha , Younas Afifa , Yousaf Zubaida , Aftab Zille Huma , Riaz Nadia , Shamsheer Bushra , Yasin Hamna 
Published: March 2022

Abstract
Quantitative trait loci (QTL) mapping is a genome-wide inference of the relationship between genotype at various genomic locations and phenotype for a set of quantitative traits in terms of the number, genomic positions, effects, and interaction of QTL. The Quinones and phenolics are the products of Geranyl diphospohate synthase (GPPS). Thymoquinone (TQ), dithymoquinone (DTQ), thymohydroquinone (THQ), and thymol (THY) are the most important pharmacologically active quinone based constituents in Nigella sativa (Black seed oil). Quantitative trait loci, candidate genes and associated morphological markers for these traits have been identified and located in the N.sativa genome. The breeding and genetic improvement of N. sativa is highly restricted due to unexplored high-density genetic map. The study was planned to map the genes linked to quinonens ane phenolics biosynthesis in N. sativa. Two inbred parental lines W626529 and W626530 differing in some phenotypic characters were selected for the study. To construct genetic linkage map and locate QTLs, F1 population of 150 crossed individuals was grown and subsequently produced 50 recombinant inbred lines (RIL). Chromosomal linkage map was constructed using 719 polymorphic pairs; consisted of 14 morphological markers and 705 SSRs through Join Map ver. 3.0. Chromosomal mapping was done using pseudochromosome assembly using Win QTL Cart Software. Single Marker Analysis was done to link QTLs with the morphological and molecular markers. Only 5 morphological traits out of 16 were found to be linked to the QTL. Chromosomal marker linkage was ensured at a minimum likelihood of odds (LOD) > 1.5 and maximum distance 50 cM. Interval mapping led to the detection of eight QTLs on 12 linkage groups; LG1, LG2, LG5, LG6, LG8, LG9 and LG11. The detection of eight QTLs on 12 linkage groups confirmed the influence of both morphological and molecular markers on linked traits and inheritance. The genetic map and improved pseudo-chromosome scale genome provide crucial foundations for marker-assisted selection (MAS) of QTL governing important agronomic traits as well as the accelerated molecular breeding of N. sativa in a cost-effective pattern.
Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana
Authors: Jiang Lingmin , Peng Yuxin , Seo Jiyoon , Jeon Doeun , Jo Mi-Gyeong , Lee Ju Huck , Jeong Jae Cheol , Kim Cha Young , Park Hyeong Cheol , Lee Jiyoung 
Published: March 2022

Abstract
A novel Gram-stain-positive, aerobic bacterial strain, designated AK-R2A1-2T, was isolated from surface-sterilized needle leaves of an Abies koreana tree. Strain AK-R2A1-2T was closely related to Subtercola boreus K300T and Subtercola lobariae 9583bT with 97.3% and 96.7% 16S rRNA gene sequence similarities, respectively, and it formed a distinct phyletic lineage from these two strains. Growth of strain AK-R2A1-2T was observed at 4–25°C with pH 5.0–8.0. Strain AK-R2A1-2T contained menaquinone 9 (MK-9) and menaquinone 10 (MK-10) as the predominant respiratory quinones. The major cellular fatty acids were anteiso-C15:0 and summed feature 8 (C18:1ω7c or/and C18:1ω6c), and the polar lipids comprised diphosphatidylglycerol (DPG), and three unknown aminolipids (AKL2, AKL3, and AKL4). The complete genome of strain AK-R2A1-2T was sequenced to understand the genetic basis of the survival strategy of the strain in low temperatures. Multiple copies of cold-associated genes involved in cold-active chaperon, stress response, and DNA repair supported survivability of the strain at low temperatures. Finally, this strain could significantly improve rice seeding growth under low temperatures. Strain AK-R2A1-2T is concluded to represent a novel species of the genus Subtercola, and the proposed name is Subtercola endophyticus sp. nov. The type strain is AK-R2A1-2T (= KCTC 49721T = GDMCC 1.2921T).
Physical mapping of qDTH3 for heading date reveals the evolutionary history of cultivated rice
Authors: Ikegaya Tomohito , Shirasawa Kenta , Fujino Kenji 
Published: March 2022

Abstract
Crop breeding programs can improve traits associated with desirable phenotypic characteristics to different local environmental conditions. In rice, such improvements were achieved depending on genetic diversity among local populations. Here, we examined whether differences in traits of elite varieties are due to genetic differences and from what the genetic differences are derived. Previously, we identified one quantitative trait locus associated with heading date, i.e., the quantitative trait locus for days to heading on chromosome 3 (qDTH3). Using 592 plants of the F2 generation, fine mapping narrowed the qDTH3 locus down to a 158-kb region of the corresponding rice reference genome. Based on the genome sequences of a 656-kb region including the qDTH3 locus among four Oryza species populations, we propose the historic evolutionary process of cultivated rice, considering nucleotide polymorphisms and intrapopulation recombination. Phenotypic differences in qDTH3 in elite varieties may be derived from a different lineage split from the ancestor.
A potential decision-making algorithm based on endoscopic ultrasound for staging early gastric cancer: A retrospective study
Authors: YAN YAN , Ma Zhonghua , Ji Xin , Ji Ke , Li Shijie , Wu Qi 
Published: March 2022

Abstract
Background Clinical staging of gastric cancer (GC) before treatment is essential. Endoscopic ultrasound (EUS) is a recommended staging tool, but its efficacy remains controversial. Our previous prospective study evaluated the potential value of EUS for T staging and presented discrepancies. In this study, we aimed to evaluate the efficacy of EUS in T staging by comparing it with pathological staging. We analyze the factors that can potentially affect accuracy to identify suitable subgroups for EUS staging.Methods Data from a total of 1763 consecutive patients with GC from January 2015 to December 2017 were analyzed. Results from EUS and pathological T staging were compared. The factors that might affect EUS’s accuracy were analyzed.Results The sensitivity, specificity, positive predictive value, and negative predictive value of EUS in patients with early GC were 62.08%, 96.13%, 90.94%, and 80.21%, respectively. The accuracy rates of uT1, uT2–uT4, and uT3–uT4 were 90.94%, 79.02%, and 78.39%, respectively. In multivariate analysis, underestimation was more likely to be observed in patients with tumors located in the middle or upper third of the stomach. Overestimation was more likely to be observed in patients with tumors located in the lower third or those without ulcer. Other factors affecting accuracy included ulcer, differentiation, larger size and undergoing surgery.Conclusion Our findings highlight the role of EUS in determining the T staging of GC. Overestimation and underestimation in T-staging were significantly associated with the tumor location in early GC, and a decision-making algorithm was proposed for clinical practice in early cancers based on these findings.
Gynecological cancer patients’ experiences with eHealth technology and partly nurse-led consultations in a follow-up setting: A qualitative study
Authors: Skorstad Mette , Ingvild Vistad , Berntsen Sveinung , Johannessen Berit 
Published: March 2022

Abstract
Background
In the last decade, there has been a move toward personalized care, with a focus on the diversity of survivorship needs after initial cancer treatment. Empowering patients to actively participate in their own health management and survivorship care is encouraged. Consequently, a new follow-up model, called Lifestyle and Empowerment Techniques in Survivorship of Gynecologic Oncology (LETSGO), for gynecological cancer patients was developed and piloted at a Norwegian hospital. In LETSGO, a dedicated nurse replaced the physician in every second follow-up consultation. The nurse provided the patients with self-management techniques following cancer treatment, reinforced with eHealth technology (a specially designed app). Encouragement of behavioral change and evaluation of the late effects of treatment and recurrence symptoms were central aspects of the self-management techniques. The app further encouraged physical activity and positive lifestyle change, helped identify recurrence-related symptoms, and provided reminders on activity goals. This study aimed to investigate the views on alternating nurse-led and physician-led care supported by eHealth technology among participants who piloted the LETSGO intervention.
Methods
Semi-structured qualitative interviews were conducted to analyze how the participants experienced the LETSGO intervention 6–7 months after the intervention onset.
Results
All LETSGO pilot study’s participants felt safe and well taken care of. They perceived the nurses as being less busy than doctors, which made it easy for them to bring up any cancer-related challenges. Most participants reported increased empowerment and confidence in being able to recognize symptoms of cancer recurrence. Half of the participants used the app regularly and were motivated to increase their levels of physical activity. Some experienced technical errors with the app.
Conclusions
Overall, partly nurse-led consultations and eHealth technology were well-received among the participants. An intervention study is required for further evaluation. The reported technical app errors should be resolved and tested prior to eHealth application implementation. This study reports gynecological cancer patients’ perspectives of nurse-led follow-up and the use of eHealth technology, and is expected to be useful in personalized survivorship care planning.
Trial registration:
ClinicalTrials.gov, NCT03453788. Registration 5 March 2018, https://clinicaltrials.gov/ct2/show/NCT03453788
The pandemic within the pandemic: the surge of neuropsychological disorders in Italian children during the COVID-19 era.
Authors: Bozzola Elena , Ferrara Pietro , Spina Giulia , Villani Alberto , Roversi Marco , Raponi Massimiliano , Corsello Giovanni , Staiano Annamaria 
Published: March 2022

Abstract
BackgroundQuarantine and isolation measures during COVID-19 pandemic may have caused additional stress and challenged mental health of the youth. Aim of the study is to investigate the COVID-19 pandemic impact on neuropsychological disorders (NPD) of Italian children and adolescents to provide general pediatric recommendations.Material and methodsA retrospective multicenter observational study was planned by the Italian Pediatric Society (SIP) to explore the impact of COVID-19 on the access of children to pediatric Emergency Departments (pED) for the evaluation of neuropsychological symptoms, collecting the classification codes of diagnoses between March 1, 2019 and March 2, 2021. The period study was split into two sub-periods: a pre COVID-19 period (from March 1 2019 to March 1, 2020) and a COVID-19 period (from March 2, 2020 to March 2, 2021). As additional information, data on NPD hospitalizations in any pediatric department of the involved centers were recorded.ResultsDuring the study period, a total of 533,318 children were admitted to the pED involved in the study. Despite a 48.2% decline of pED admissions, there was a significant increase (83.1%) in patient admissions for NPD. The most frequent NPD conditions which increased during the COVID-19 pandemic were suicidal ideation (+ 147%), depression (+ 115%), eating disorder (+ 78.4%), and psychosis (+ 17.2%). During the pandemic period, a 39.5% increase in NPD hospitalizations was observed as well. The NPD disorders that mostly required hospitalizations were suicidal ideation (+ 134%), depression (+ 41.4%), eating disorder (+ 31.4%), and drug abuse (+ 26.7%).DiscussionThe COVID-19 pandemic had a major impact on children's health, mainly on their NPD development. Neuropsychological assessment should be required at the primary level, in the pediatrician's office, to facilitate early capture of the sign of impairment and provide an adequate treatment.Conclusion. SIP underlines the psychological consequences of COVID 19 pandemic on the youngest and recommend an early identification of NPD in the pediatric population to avoid other serious consequences for children's physical and mental health.
Dynamic stability of salt stable cowpea chlorotic mottle virus capsid protein dimers and pentamers of dimers
Authors: Fejer Szilard , Szövérfi János 
Published: March 2022

Abstract
Parts of the self-assembly process of the salt stable cowpea chlorotic mottle virus (ss-CCMV) capsid can be modelled atomistically on realistic computational timescales only if we focus on the dissociation of protein complexes instead of their aggregation. The aim of the current study is to find and understand the conditions that lead to the separation of ss-CCMV capsid protein dimers. Our previous studies showed that among the three possible interfaces in the icosahedral capsid, two (T1 and T2) are important during capsid formation. Long timescale molecular dynamics simulations of the various dimers and the pentamer of dimers underscore the importance of large contact surfaces on stabilizing the capsid subunits within an oligomer. At higher temperature, the N-terminal tails of the protein units act as tethers, delaying dissociation for all but the most stable (T1) interfaces, therefore their flexibility likely has an important role during the self-assembling process, as tethering increases the effective concentration of the monomers and the likelihood of finding native contacts. The pentamer of dimers is also found to be stable on long timescales, with an inherent flexibility of the outer protein chains.
Wall Shear Stress Differences Between Arterial and Venous Coronary Artery Bypass Grafts One Month After Surgery
Authors: Tran-Nguyen Nhien , Condemi Francesca , Yan Andrew , Fremes Stephen , Triverio Piero , Jimenez-Juan Laura 
Published: March 2022

Abstract
Although coronary artery bypass graft (CABG) surgery is a well-established intervention, graft failure can occur, and the underlying mechanisms remain incompletely understood. The purpose of this prospective study is to utilize computational fluid dynamics (CFD) to investigate how graft hemodynamics one month post surgery may vary among graft types, which have different long-term patency rates. Twenty-four grafts from 10 participants (64.6 ± 8.5 years, 9 men) were scanned with coronary CT angiography and 4D flow MRI one month after CABG surgery. Grafts included 10 left internal mammary arteries (LIMA), 3 radial arteries (RA), and 11 saphenous vein grafts (SVG). Image-guided CFD was used to quantify blood flow rate and wall area exposed to abnormal wall shear stress (WSS). Arterial grafts had a lower abnormal WSS area than venous grafts (17.9% vs. 70.1%; p = 0.001), and a similar trend was observed for LIMA vs. SVG (13.8% vs. 70.1%; p = 0.001). Abnormal WSS area correlated positively to lumen diameter (p < 0.001) and negatively to flow rate (p = 0.001). This CFD study is the first of its kind to prospectively reveal differences in abnormal WSS area 1 month post surgery among CABG types, suggesting that WSS may influence the differential long-term graft failure rates observed among these groups.
Calculation of tooth thickness errors and its influence on meshing backlash of harmonic drive
Authors: Yang Congbin , Ma Honglie , Zhang Tao , Zheng Jigui , Liu Zhifeng , Cheng Qiang 
Published: March 2022

Abstract
Meshing backlash mainly determines the transmission accuracy of harmonic drive, which is an important index in design. In this paper, a design method of short tooth involute profile is proposed, which relies on positive modification to enhance the strength of dedendum. By intercepting part of tooth profile, the meshing interference of addendum can be effectively avoided. Based on the dimensions of over pins of circular spline and flexspline, a calculation method of tooth thickness deviation is proposed, and the positive and negative of tooth thickness deviation are defined. Involute functions need not be calculated by this method, which simplifies the solution process. By discretizing the teeth profile points of circular spline and flexspline, a calculation method of time-varying backlash is proposed, the variation of backlash in the process of meshing in and out is analyzed, and the influence law of tooth thickness deviation on backlash is studied. On this basis, an algorithm of adjusting the radial deformation to compensate the backlash is proposed. The results show that the excessive backlash in the meshing area can be reduced by increasing the radial deformation. when meshing interference occurs, the interference free meshing can be realized by decreasing the radial deformation.
Laboratory study of adsorption kinetics of benzene and toluene contaminants on copper oxide nanoparticles
Authors: Farahbod Farshad 
Published: March 2022

Abstract
In recent years, various studies have been defined on the removal of contaminants from the soil. This shows the importance of this issue and human attention to a healthy ecosystem and human health. The petroleum derivatives are one of the most important pollutants in industrial effluents, especially oil effluents. In this study, the soil contaminated with oil derivatives was washed and then the resulting effluent was in contact with copper oxide nanoparticles. In this study, the concentrations of benzene and toluene in the effluent are 5.8, 6.3, 6.9, 7.2, 8.1, 8.7, 9.8, 10.3, 10.8, 11.2, 11.5 and 12.1 mg/l, respectively. In this work, the effect of variables such as initial concentration of pollutants, time and acidity on the absorption efficiency of these pollutants was investigated. Laboratory results indicate that the highest removal efficiencies of benzene and toluene occur at concentrations of 6.3 and 6.9, respectively. Also, other results show that the maximum adsorption of benzene and toluene occurs on the surface of copper oxide nanoparticles if the acidity of the effluent is 9.7 and 5.6, respectively. The presented results show that the equilibrium data of adsorption of benzene and toluene pollutants follow the quadratic kinetic model and intraparticle diffusion model.
Experimental Evidence for E = n 1 2 hν Energy Transfer
Authors: P. Bhanumoorthy 
Published: March 2022

Abstract
Crystal diffraction involves energy transfer into and from the crystal. Bragg’s law defines an inter-planar spacing as 2π radians and corresponds to E = 12.4/λ which is E = nhν energy transfer. Energy interval between adjacent peaks measured in the historic Davisson-Germer experiment demonstrated E = 6.2/λ which is E = n 1 2 hν energy transfer. One-full oscillation in 2π radians is one-full quantum energy and one-half oscillation in π radians is one-half quantum energy. The phenomenon of E = n 1 2 hν energy transfer defines the Maxwell diffraction law 2dk sin θk = λ in terms of diffraction angle θk where inter-planar spacing dk becomes π radians characteristic of an anti-node of a standing wave. Cartesian description cannot accommodate π in place of 2π. Spherical polar coordinate description alone can include angle dependence measured in the experiment to determine orbital structure and bonding schemes of individual atoms. The Maxwell diffraction law in place of Bragg’s law becomes the starting point for the new field of partial wave quantum crystallography to use experimental precision angles for direct method solution to phase problem for macromolecular crystallography
Fe[4-(3-Phenylpropyl)pyridine]2[Fe(CN)5NO]: A 2D coordination polymer with thermally-induced spin transition and nature of its asymmetric hysteresis loop
Authors: Scanda K. , Avila Y. , Mojica R. , Cano A. , Gonzalez M. , Rodríguez-Hernández Joelis , Reguera Edilso 
Published: March 2022

Abstract
The titled material crystallizes with an orthorhombic unit cell, in the P21212 space group (Nr. 18). Its crystal structure was solved and refined from XRD powder patterns. The solid framework is formed by stacked undulated sheets of inorganic nature, Fe[Fe(CN)5NO], separated by bimolecular organic pillars, [4-(3-Phenylpropyl)pyridine]2, which remain coordinated to the axial coordination sites for the iron atom and occupy the interlayer region. The molecules forming these pillars remain coupled through C-H⋅⋅⋅π and dispersive interactions between neighboring molecules. When this solid is cooled and then warming, a reversible spin transition, from high to low spin (HS → LS), and vice versa, is observed. This transition occurs in the temperature interval of 135–165 K, with a hysteresis between them of about 20 K. That hysteresis loop appears with a pronounced asymmetry when the slopes for the HS → LS and LS → HS transitions are compared. This effect is discussed in terms of the related structural changes in the solid structure during the spin transitions. The transition was also monitored from IR and Raman spectra recorded at 80 and 300 K. Relevant information on the electronic structure for both, the LS and HS phases of this material, was derived from the corresponding XPS spectra recorded at 114 and 270 K. This contribution emphasizes the role of the nitrosyl group (NO) as an electron buffer for tuning the bonding properties of the inorganic layer at the CN 5σ orbital to make possible the observed thermally-induced SCO behavior.
An anatomical and radiological study of the tectorial membrane and supradental venous plexus: its clinical significance in traumatic craniovertebral dislocation
Authors: Lee Shin Hyo , Cho Tae-Hyeon , Kwon Hyun-Jin , Hong Ju Eun , Lee Young Han , Yang Hun-Mu 
Published: March 2022

Abstract
The radiological image of an intact tectorial membrane (TM) became an important favorable prognostic factor for craniovertebral instability. This study visualized the fascial layers of the TM and adjacent connective tissues with clinical significance by micro-CT and histological analysis. The TM firmly attached to the bony surface of the clivus, traversed the atlantoaxial joint posteriorly, and was inserted to the body of the axis showing wide distribution on the craniovertebral junction. The supradental space between the clivus, dens of the axis, anterior atlantooccipital membrane, and the TM contained profound venous networks within the adipose tissues. At the body of the axis, the compact TM layer is gradually divided into multiple layers and the deeper TM layers reached the axis while the superficial layer continued to the posterior longitudinal ligament of the lower vertebrae. The consistent presence of the fat pad and venous plexus in the supradental space and firm stabilization of the TM on the craniovertebral junction was demonstrated by high-resolution radiologic images and histological analysis. The evaluation of the TM integrity is a promising diagnostic factor for traumatic craniovertebral dislocation.