bluuebunny/crossref_metadata_2025_split huggingface dataset:
PEMBERIAN BERBAGAI DOSIS PUPUK NPK DAN PUPUK KANDANG KAMBING TERHADAP PERTUMBUHAN DAN HASIL TANAMAN SORGUM ( Sorghum bicolor L.)
Authors: Choirunnisa Pebriana , Ratna Nurhayati Dewi , Triyono Kharis 
Published: August 2022

Penelitian ini berjudul Pengaruh Pemberian Berbagai Dosis Pupuk NPK Dan Pupuk Kandang Kambing Terhadap Pertumbuhan Dan Hasil Tanaman Sorghum (Sorgum bicolor L), bertujuan untuk mengetahui pengaruh pemberian berbagai dosis pupuk NPK dan pupuk kandang kambing terhadap pertumbuhan dan hasil tanaman sorgum, dilaksanakan mulai bulan Mei 2021 sampai bulan Agustus 2021, di Green House atau Kebun Percobaan Fakultas Slamet Riyadi Surakarta, yang berlokasi di Kelurahan Kadipiro, Kec. Banjarsari, Kota Surakarta, Jawa Tengah dengan ketinggian tempat 105 mdpl dan jenis tanahnya Aluvial. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang disususn secara faktorial yang terdiri dari 16 perlakuan yang masing – masing perlakuan diulang sebanyak 3 kali. Pupuk yang digunakan yaitu pupuk NPK dan pupuk kandang kambing. Data hasil penelitian ini dianalisis dengan uji BNJ (Beda Nyata Jujur) pada taraf 5 % . Parameter – parameter yang diamati meliputi tinggi tanaman, jumlah daun, berat segar biji per malai, berat kering biji per malai, berat 1000 biji, jumlah biji per malai, berat brangkasan basah, berat brangkasan kering, dan panjang malai. Hasil penelitian menunjukkan bahwa : (1) Perlakuan pupuk kandang kambing dosis 70 g/tanaman memberikan hasil terbaik terhadap parameter berat 1000 biji dan perlakuan pupuk kandang kambing dosis 105 g/tanaman memberikan hasil terbaik pada berat brangkasan basah. (1)Perlakuan pupuk NPK 8 g/tanaman memberikan hasil terbaik terhadap parameter jumlah daun, perlakuan pupuk NPK 6 g/tanaman memberikan hasil terbaik terhadap parameter berat kering biji per malai dan jumlah biji per malai. Sedangkan pupuk NPK 8 g/tanaman memberikan hasil terbaik terhadap parameter berat basah biji per malai dan berat kering brangkasan, perlakuan pupuk NPK 6 g/tanaman memberikan hasil terbaik terhadap parameter berat brangkasan basah. (3) Interaksi antara dosis pupuk kandang kambing (K) dan pupuk NPK (N) tidak memberikan pengaruh pada semua pengamatan.
Arang Sekam Dan Dosis Pupuk NPK Terhadap Pertumbuhan Dan Hasil Tanaman Jagung (Zea mays L.).
Authors: Indriyanti Sri , Priyono Priyono , Bahri Saiful 
Published: August 2022

Penelitian ini berjudul Pengaruh Arang Sekam dan Dosis Pupuk NPK Terhadap Pertumbuhan dan Hasil Tanaman Jagung (Zea mays L.), penelitian ini bertujuan untuk menganalisis pengaruh arang sekam dan dosis pupuk NPK terhadap pertumbuhan dan hasil tanaman jagung, yang dilaksanakan mulai bulan Agustus – November 2021. Penelitian dilakukan di Mendalan, Jipangan, Banyudono, Boyolali dengan ketinggian tempat 158 mdpl. Jenis tanah yang digunakan adalah tanah regosol. Penelitian ini menggunakan metode Rancangan Acak Kelompok Lengkap (RAKL) yang disusun secara factorial yang terdiri dari 10 perlakuan yang masing – masing perlakuan diulang sebanyak 3 kali. Pupuk yang digunakan yaitu arang sekam dan pupuk NPK. Data hasil penelitian ini dianalisis dengan uji LSD (Least Significant Difference) pada taraf 5 %. Parameter yang diamati meliputi tinggi tanaman, jumlah daun, jumlah tongkol, berat tongkol dengan kelobot, berat tongkol tanpa kelobot, berat brangkasan basah, berat brangkasan kering. Hasil penelitian menunjukkan bahwa 1. Pada fase pertumbuhan tanaman, perlakuan arang sekam dan dosis pupuk NPK memberikan pengaruh pada tinggi tanaman dan berat brangkasan kering. Perlakuan tanpa arang sekam 2 kg/tan dan dosis pupuk NPK 1,16 g/tanaman (A0N3), pada tinggi tanaman menghasilkan rata - rata 266,37 cm dan berat brangkasan kering arang sekam 2 g/tanaman dan dosis pupuk NPK 1,23 g/tanaman (A1N4) dengan rata – rata yaitu 434,33 g/tanaman. Sedangkan pada jumlah daun, berat brangkasan basah sedikit mempengaruhi (kecenderungan) yang memberikan hasil berbeda. Pada jumlah daun tanpa arang dan dosis pupuk NPK 1,16 g/tanaman (A0N3) dengan hasil yaitu 15,33 helai dan berat berangkasan basah arang sekam 2 g/tanaman dan dosis pupuk NPK 0 g/tanaman (A1N0) dengan rata – rata tertinggi 984,77 gram. 2. Pada hasil panen, perlakuan arang sekam dan dosis pupuk NPK tidak memberikan pengaruh pada perlakuan tanpa arang sekam dan dosis pupuk NPK 1,01 g/tanaman (A0N1), pada jumlah tongkol menghasilkan rata-rata 2,83 buah, berat tongkol dengan kelobot arang sekam 2 kg/tanaman dan dosis pupuk NPK 0 g/tanaman menghasilkan rata-rata 31,72 gram dan berat tongkol tanpa kelobot perlakuan tanpa arang sekam dan dosis pupuk NPK 1,16 g/tanaman (A0N3) menghasilkan rata-rata 20,26 gram.
Kata kunci : Arang Sekam, NPK, Pertumbuhan, Hasil, Jagung
Adaptive Immune Response to Mycobacterium abscessus Complex (MABSC) in Cystic Fibrosis and the Implications of Cross-Reactivity
Authors: Mauch Renan Marrichi , Jensen Peter Østrup , Qvist Tavs , Kolpen Mette , Moser Claus , Pressler Tacjana , Nolasco da Silva Marcos Tadeu , Høiby Niels ,   The Copenhagen Study Group on Mycobacterial Infections in Cystic Fibrosis
Published: August 2022

BackgroundWe aimed to characterise the adaptive immune response to Mycobacterium abscessus complex (MABSC) and its cross-reactivity with Mycobacterium avium complex (MAC) and Mycobacterium bovis (Bacille Calmette-Guérin, BCG) in cystic fibrosis (CF) patients and non-CF controls in terms of lymphocyte proliferation and immunophenotyping, cytokine production and anti-MABSC IgG plasma levels.MethodsIn this cross-sectional analysis, peripheral blood mononuclear cells (PBMC) from CF patients with MABSC (CF/MABSC, n=12), MAC infection history (CF/MAC, n=5), no NTM history (CF/NTM-, n=15), BCG-vaccinated (C/BCG+, n=9) and non-vaccinated controls (C/BCG-, n=8) were cultured for four days under stimulation with an in-house MABSC lysate and we used flow cytometry to assess lymphocyte proliferation (given by lymphoblast formation) and immunophenotypes. Cytokine production was assessed after overnight whole blood stimulation with the same lysate, and anti-MABSC IgG levels were measured in plasma from non-stimulated blood.ResultsAll CF/MABSC patients had increased CD3+ and CD19+ lymphoblast formation upon PBMC stimulation with MABSC lysate. There was a higher rate of CD3+ than CD19+ lymphoblasts, predominance of CD4+ over CD8+ lymphoblasts, IFN-γ, TNF-α and IL-2 production, low production of the Th17-associated IL-17, and discrete or no production of Th2/B cell-associated cytokines soluble CD40 ligand (CD40L), IL-4 and IL-5, indicating a Th1-dominated phenotype and infection restricted to the lungs. A similar pattern was seen in C/BCG+ controls, and CF/MAC patients, pointing to cross-reactivity. MABSC-IgG levels were higher in CF/MABSC patients than in both control groups, but not CF/NTM- patients, most of whom also had CD3+ and/or CD19+ lymphoblast formation upon PBMC stimulation, indicating previous exposure, subclinical or latent infection with MABSC or other NTM.ConclusionThe anti-MABSC immune response is Th1-skewed and underlines the cross-reactivity in the anti-mycobacterial immune response. The results, together with published clinical observations, indicate that BCG vaccination may cross-react against NTM in CF patients, and this should be investigated. Due to cross-reactivity, it would also be interesting to investigate whether a combination of MABSC-induced cytokine production by blood cells and anti-MABSC IgG measurement can be useful for identifying latent or subclinical infection both with MABSC and other NTM in CF patients.
Energy fluxes, mass balance, and climate sensitivity of the Sutri Dhaka Glacier in the western Himalaya
Authors: Oulkar Sunil N. , Thamban Meloth , Sharma Parmanand , Pratap Bhanu , Singh Ajit T. , Patel Lavkush Kumar , Pramanik Ankit , Ravichandran M. 
Published: August 2022

Various regional climatic factors influence glacier mass balance and thus control the water budget of the Himalayan rivers. However, the scarcity of observational data hinders a detailed understanding of the processes governing glacier mass balances in the Himalaya. Here we analyze the mass balance of the Sutri Dhaka Glacier, a debris-free glacier in the Chandra basin (western Himalaya) combining field observations and a physically based model to understand the drivers of mass balance variability. The modeled energy flux showed that net shortwave radiation contributed 56% to the total surface energy fluxes, followed by net longwave radiation (27%), sensible heat (8%), latent heat (5%), and ground heat flux (4%). However, over the ablation zone, inward fluxes account for most of the total heat flux, resulting in strong summertime melting. The model estimated glacier mass balance was −1.09 ± 0.31 and −0.62 ± 0.19 m w.e. during 2015/16 and 2016/17, which matches well with the in situ glaciological mass balance of −1.16 ± 0.33 and −0.67 ± 0.33 m w.e., respectively. A sensitivity analysis demonstrates that the mass balance of the glacier is affected by both air temperature (−0.21 m w.e. a−1 °C−1) and precipitation (0.19 m w.e. a−1 (10%)−1) changes. Our study suggests that, the mass balance of the Sutri Dhaka Glacier is less sensitive to changes in the partitioning of precipitation into snow and rain because the majority of precipitation falls as snow during the winter when the temperature is well below 0°C.
"Double Frozen Transfer" Could Influence the Perinatal and Children's Growth: A Nested Case-Control Study of 6705 Live Birth Cycles
Authors: Gao Jie , Zhang Yiyuan , Cui Linlin , Zhang Tao , Wu Bingjie , Gao Shanshan , Chen Zi-Jiang 
Published: August 2022

ObjectiveThis study aims to evaluate neonatal and children growth outcomes of cryotransfer of embryos developed from frozen gametes [double frozen transfer (DFT)].MethodsThis nested case-control study included 6,705 women who had a singleton live birth after embryo transfer at the Center for Reproductive Medicine, Shandong University, from 2008 to 2020. Of these, 745 women underwent frozen embryo transfer (FET) using embryos developed from frozen gametes (DFT). Propensity score methodology was used to balance the two groups by maternal age and body mass index (BMI) before evaluating outcomes. After age and BMI were matched using the propensity score methodology in a ratio of 1:4, the control groups enrolled 2,980 women who underwent fresh embryo transfer (ET) and 2,980 women underwent FET from fresh gametes. The children born were followed to at least 5 years of age, and some were followed up to 10 years. Neonatal outcomes and childhood growth measurements were compared among the three groups.ResultsThe average birth weight of the DFT group (3,462 g) was significantly higher than the FET group (3,458 g) and ET group (3,412 g). The rate of large for gestational age (LGA) babies in the DFT and FET group was higher than that for the ET group (30.9% vs. 24.8%; 29.4% vs. 24.8%, respectively). After adjusting for different confounder combinations in the three models, the birth weight and risk of LGA in the DFT and FET groups were still higher than in the ET group, and the values group of P for trend in the models were significant. In multiple linear regression analysis of the children’s development, the height Z-score of children born from the DFT and FET group was higher than that for children from the ET group (β = 0.21, 95% CI 0.07–0.35; b = 0.17, 95% CI 0.05–0.28, respectively). However, childhood growth measurements including body weight Z-score and BMI Z-score were not significantly different among the three groups. In addition, the proportion of male children born from DET was higher than that from ET.ConclusionsThere is an increased risk of LGA babies associated with pregnancies conceived from DFT. Children are inclined to be taller in the future in this group than after FET. The related etiology and pathophysiology mechanisms still need to be revealed. In the future, well-designed, observational studies with in-depth collection of patients’ characteristics may shed more light on this issue.
The Causal Evidence of Birth Weight and Female-Related Traits and Diseases: A Two-Sample Mendelian Randomization Analysis
Authors: He Renke , Liu Rui , Wu Haiyan , Yu Jiaen , Jiang Zhaoying , Huang Hefeng 
Published: August 2022

Objectives: A large meta-analysis indicated a more pronounced association between lower birth weight (BW) and diseases in women but less concern about the causality between BW and female-related phenotypes and diseases.Methods: Mendelian randomization (MR) analysis was used to estimate the causal relationship between two traits or diseases using summary datasets from genome-wide association studies. Exposure instrumental variables are variants that are strongly associated with traits and are tested using four different statistical methods, including the inverse variance weighting, MR-Egger, weighted median, and weighted mode in MR analysis. Next, sensitivity analysis and horizontal pleiotropy were assessed using leave-one-out and MR-PRESSO packages.Results: The body mass index (BMI) in adulthood was determined by BW (corrected β = 0.071, p = 3.19E-03). Lower BW could decrease the adult sex hormone-binding globulin (SHBG) level (β = −0.081, p = 2.08E-06), but it resulted in increased levels of bioavailable testosterone (bio-T) (β = 0.105, p = 1.25E-05). A potential inverse effect was observed between BW and menarche (corrected β = −0.048, p = 4.75E-03), and no causal association was confirmed between BW and the risk of endometriosis, leiomyoma, and polycystic ovary syndrome.Conclusion: Our results suggest that BW may play an important role and demonstrates a significant direct influence on female BMI, SHBG and bio-T levels, and menarche.
Next-generation sequencing reveals a case of Norrie disease in a child with bilateral ocular malformation
Authors: Li Haijun , Li Zhiming , Wang Degang , Chen Chuanming , Chen Zhiqiang , Wang Jinhua , Xu Chenxia , Dong Xingsheng 
Published: August 2022

A Norrie disease protein gene (NDP) variant, c.174 + 1G &gt; A, was found in a Chinese family through next-generation sequencing and verified with Sanger sequencing. A case of Norrie disease was reported in the first child, and the symptoms were consistent with the results of gene sequencing. The child’s mother, who was pregnant at the time, was found to be a carrier of the identified pathogenic variant. To determine if the fetus carried the same disease-causing variant, prenatal examination and prenatal diagnosis were conducted. The fetus had biocular vitreous abnormalities and complete retinal abnormalities. Genetic testing showed that the fetus had maternally inherited the NDP gene variant found in the proband. It was concurrently confirmed that the NDP gene variant led to the deletion of 246 bp at the 3′ end of exon 2, resulting in the deletion of the initiation codon and the occurrence of disease. Our study suggests that the diagnosis of rare diseases through next-generation sequencing, combined with prenatal ultrasound and prenatal diagnosis, can help families with known familial genetic diseases. Furthermore, the findings of this study broaden the known genetic spectrum of Norrie disease.
Comprehensive Analysis of Molecular Subtypes and Hub Genes of Sepsis by Gene Expression Profiles
Authors: Lai Yongxing , Lin Chunjin , Lin Xing , Wu Lijuan , Zhao Yinan , Shao Tingfang , Lin Fan 
Published: August 2022

Background: Sepsis is a systemic inflammatory response syndrome (SIRS) with heterogeneity of clinical symptoms. Studies further exploring the molecular subtypes of sepsis and elucidating its probable mechanisms are urgently needed.Methods: Microarray datasets of peripheral blood in sepsis were downloaded from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were identified. Weighted gene co-expression network analysis (WGCNA) analysis was conducted to screen key module genes. Consensus clustering analysis was carried out to identify distinct sepsis molecular subtypes. Subtype-specific pathways were explored using gene set variation analysis (GSVA). Afterward, we intersected subtype-related, dramatically expressed and module-specific genes to screen consensus DEGs (co-DEGs). Enrichment analysis was carried out to identify key pathways. The least absolute shrinkage and selection operator (LASSO) regression analysis was used for screen potential diagnostic biomarkers.Results: Patients with sepsis were classified into three clusters. GSVA showed these DEGs among different clusters in sepsis were assigned to metabolism, oxidative phosphorylation, autophagy regulation, and VEGF pathways, etc. In addition, we identified 40 co-DEGs and several dysregulated pathways. A diagnostic model with 25-gene signature was proven to be of high value for the diagnosis of sepsis. Genes in the diagnostic model with AUC values more than 0.95 in external datasets were screened as key genes for the diagnosis of sepsis. Finally, ANKRD22, GPR84, GYG1, BLOC1S1, CARD11, NOG, and LRG1 were recognized as critical genes associated with sepsis molecular subtypes.Conclusion: There are remarkable differences in and enriched pathways among different molecular subgroups of sepsis, which may be the key factors leading to heterogeneity of clinical symptoms and prognosis in patients with sepsis. Our current study provides novel diagnostic and therapeutic biomarkers for sepsis molecular subtypes.
Utilization of hypoxia-derived gene signatures to predict clinical outcomes and immune checkpoint blockade therapy responses in prostate cancer
Authors: Chen Minhua , Chen Zhang , Lin Zongbin , Ding Xiang , Liang Tianyu 
Published: August 2022

Background: Increasing evidences show a clinical significance in the interaction between hypoxia and prostate cancer. However, reliable prognostic signatures based on hypoxia have not been established yet.Methods: We screened hypoxia-related gene modules by weighted gene co-expression network analysis (WGCNA) and established a hypoxia-related prognostic risk score (HPRS) model by univariate Cox and LASSO-Cox analyses. In addition, enriched pathways, genomic mutations, and tumor-infiltrating immune cells in HPRS subgroups were analyzed and compared. HPRS was also estimated to predict immune checkpoint blockade (ICB) therapy response.Results: A hypoxia-related 22-gene prognostic model was established. Furthermore, three independent validation cohorts showed moderate performance in predicting biochemical recurrence-free (BCR-free) survival. HPRS could be a useful tool in selecting patients who can benefit from ICB therapy. The CIBERSORT results in our study demonstrated that hypoxia might act on multiple T cells, activated NK cells, and macrophages M1 in various ways, suggesting that hypoxia might exert its anti-tumor effects by suppressing T cells and NK cells.Conclusion: Hypoxia plays an important role in the progression of prostate cancer. The hypoxia-derived signatures are promising biomarkers to predict biochemical recurrence-free survival and ICB therapy responses in patients with prostate cancer.
Comparison of PuraStat self-assembling peptide hydrogel versus mineral-based Hemospray for endoscopic hemostasis of upper and lower gastrointestinal lesions in pigs
Authors: Gil Eun Seok , O’Neill Kate , Aleksi Elton , Budrewicz Jay , Melidone Raffaele , Spirio Lisa 
Published: August 2022

ObjectiveTo compare a RADA16-based self-assembling peptide hydrogel versus an inorganic powder-based spray device for controlling postoperative bleeding in upper and lower GI mucosal lesions in pigs.MethodsMultiple mucosal lesions were endoscopically-created in the stomachs and lower colons of six Yorkshire swine on Day 0. Three animals’ wounds were treated with 2.5% RADA16 solution (PuraStat®), two animals were treated with an aerosolized mineral powder (Hemospray®), and one animal was an untreated control. Primary outcomes were test article applications required to control initial bleeding, time-to-hemostasis, and rebleeding incidence. Secondary outcomes included animal recovery, and clinical pathology at weekly endoscopic evaluations and the 4-week study terminus.ResultsNumber of material administrations required and time-to-hemostasis was comparable between PuraStat and Hemospray groups. Rebleeding rates were comparable between treatments. Two of 12 (17%) Hemospray and none of 18 (0%) PuraStat stomach sites experienced rebleeding during the final 4 min of the 10-min observation period. No delayed bleeding was observed during weekly endoscopic follow-ups. Hematology and serology values remained normal in all animals. Histology showed expected healing responses at all PuraStat- and Hemospray-treated defects, with less inflammation than untreated sites. Histomorphological observations were comparable between different groups for both the stomach and colon for test and control materials, with lower inflammation scores than untreated sites. Performance and usability responses were generally good with both systems, although the Ability to Treat Intended Site score was significantly better with PuraStat in upper GI lesions.ConclusionsPuraStat and Hemospray were effective topical hemostats for mild-to-moderate bleeding in upper and lower GI wounds. Rebleeding was observed in two of 12 Hemospray-treated sites and none of 18 PuraStat-treated sites. PuraStat and Hemospray were associated with better wound healing than untreated controls. The ability to treat upper GI lesions was easier with the PuraStat versus Hemospray system.
Display of receptor-binding domain of SARS-CoV-2 Spike protein variants on the Saccharomyces cerevisiae cell surface
Authors: Xing Hongguan , Zhu Liyan , Wang Pingping , Zhao Guoping , Zhou Zhihua , Yang Yi , Zou Hong , Yan Xing 
Published: August 2022

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), represents a significant global human health threat. The most effective way to end the pandemic is through timely vaccination. In this study, the receptor-binding domains (RBDs) of Spike protein of the initial strain of SARS-CoV-2 and its variants, B.1.1.7 (Alpha), B.1.351 (Beta), and B.1.617.1 (Kappa), were successfully displayed on the surface of a Saccharomyces cerevisiae strain for development as a vaccine candidate. To rapidly express the recombinant protein and avoid the need for expensive galactose as an inducer, the gal80 gene of S. cerevisiae was knocked out, and the conventional 72-h culture period was thus successfully shortened to 24 h. Mice vaccinated against variant B.1.617.1 showed robust humoral and cellular immune responses. Moreover, the antiserum in the B.1.671.1 group had neutralizing activity against wild-type RBD and high binding titers against RBD mutants of variants B.1.351 and B.1.1.7. Double deglycosylation at N331Q and N343Q resulted in marked reduction of the affinity of RBD binding to angiotensin converting enzyme 2 (ACE2) and escaped antibody neutralization. This study demonstrates that yeast surface display technology can provide an alternative approach to rapid large-scale preparation of promising SARS-CoV-2 vaccine candidates at low cost.