bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spanish adaptation of the Quality in Psychiatric Care-Outpatient (QPC-OP) instrument Community Mental Health Patients’ Version: Psychometric properties and factor structure
Authors: Tomás-Jiménez Manuel , Roldán-Merino Juan Francisco , Sanchez-Balcells Sara , Schröder Agneta , Lundqvist Lars-Olov , Puig-Llobet Montserrat , Moreno-Poyato Antonio Rafael , Campo Marta Domínguez del , Lluch-Canut Maria Teresa 
Published: July 2022

Abstract
Background
Health systems in the field of mental health are strongly committed to community models that allow patients to be attended in their own environment. This helps them to maintain their family and social ties while trying to avoid costly hospital admissions. The patients’ perspective is a key component in the assessment of the quality of psychiatric care and can even determine their adherence to the devices where they are treated. However, there are few instruments with adequate psychometric properties for the evaluation of the quality of psychiatric care in community mental health. The Quality in Psychiatric Care – Outpatient (QPC-OP) instrument has adequate psychometric properties to assess the quality of psychiatric care from the patients’ perspective. The aim of this study was to validate the Spanish version of the QPC-OP instrument.
Methods
A translation and back-translation of the instrument was carried out. To examine its psychometric properties, the instrument was administered to 200 patients attending various community mental health services. To assess test-retest reliability, the instrument was readministered after 7–14 days (n = 98).
Results
The Confirmatory Factor Analysis revealed a structure of 8 factors identical to the original version, with an adequate model fit. The internal consistency coefficient (Cronbach’s alpha) was 0.951. The intraclass correlation coefficient was 0.764 (95% IC: 0.649–0.842), and higher than 0.70 in 5 of the 8 factors.
Conclusions
Results show that the Spanish version of the QPC-OP instrument is valid and reliable for the assessment of quality of psychiatric care in the community setting.
Fungi associated with woody tissues of Acer pseudoplatanus in forest stands with different health status concerning sooty bark disease (Cryptostroma corticale)
Authors: Schlößer Rebekka , Bien Steffen , Langer Gitta Jutta , Langer Ewald Johannes 
Published: July 2022

Abstract
In previous years, namely 2018–2020, Germany experienced periods of exceptional weather conditions. Extremely high summer temperatures and precipitation deficits induced stress and mortality in forest trees. Acer pseudoplatanus (sycamore) was one of the affected tree species. Symptoms of the Sooty Bark Disease (SBD) and severe damage of entire stands, both caused by the invasive fungal species Cryptostroma corticale, were reported more frequently. In order to get a better understanding of the distribution of C. corticale, case reports of SBD for Germany, in general, as well as for the federal state of Hesse, in particular, are compiled and presented. To explore the endophytic distribution of C. corticale, wood cores from visibly healthy sycamore stems were sampled and all outgrowing fungi were determined and recorded. In total, 50 trees, aged 30–65 years, were sampled at five different forest stands and from which 91 endophytic filamentous fungal species could be isolated. The fungal endophytic community in the woody tissue of the sycamore trees varied greatly at the different sites and between the trees. The number of endophytic species isolated at the different sites ranged from 13 to 43 and no species was found at all sites. At 1.20 m stem height, 3.3 endophyte species could be isolated from woody tissue per tree on average. The most abundant species isolated from visibly healthy sycamore in regard to both occurrence at the studied sites and continuity was C. corticale. It was recorded at four studied forest stands, from 26% of all studied sycamore trees and had a frequency of 7.85% relative to the 293 isolated filamentous strains that were isolated. The second most abundant species was Xylaria longipes followed by Lopadostoma turgidum.
Drumming behavior of a Japanese pygmy woodpecker in Satoyama: Implications of tree felling and human settlement
Authors: Onoda Setsuko 
Published: July 2022

Abstract
The Japanese pygmy woodpecker (Dendrocopos kizuki), called “kogera” in Japanese, normally drums on trees. However, a unique behaviour of drumming on metal plates was observed in a Japanese pygmy woodpecker from observations conducted over seven years in the Satoyama landscape of Japan. The woodpecker often preferred to drum on metal plates of telephone poles in order to exploit the specific high-pitched sound reflecting from the metal. Therefore, he concluded that landscape diversity translates into behavioural diversity within species of an organism. Behaviour of the Japanese pygmy woodpecker is poorly studied, especially in the Satoyama areas. Therefore, this study aimed at assessing the influence of human development and economic growth on specific drumming behaviours of the Japanese pygmy woodpecker as well as the effect of landscape diversity on behavioural patterns and species diversity.
Transcriptomic analysis identified new lipid biomarkers for patient stratification in breast cancer
Authors: Din Zaheer Ud , Cui Bai , Wang Cenxin , Ghayas Hassan , Peng Fei , Haq Farhan , Liu Quentin 
Published: July 2022

Abstract
Background
Breast cancer is a common malignancy among women and a big challenge that causes an extensive burden on public health. Breast cancer is a heterogeneous malignancy, and the exact mechanism of development is not clear. Lipid biomarkers can play an important role in predicting the outcome of breast cancer patients. Previously different biomarkers have been identified against survival, metastasis, and prognosis. However, lipid associated biomarkers are not readily used for predicting prognosis and devising new treatment options. Therefore, we aimed to analyze lipid metabolism genes to identify the most significant lipid metabolism biomarkers in BC.
Methods
RNA-seq data of 1787 breast cancer patients were downloaded from Gene Expression Omnibus and The Cancer Genome Atlas databases. The mRNA expression of ABAT, SRD5A1, and PSAT1 was analyzed in SK-BR3, MDA-MB-231, BT-549 BC cell lines and normal breast cells. We investigated the clinicopathological parameters of lipid metabolism genes using the Kruskal-Wallis test, post-hoc Mann-Whitney-U-test, Chi-square/Fisher-exact test, Kaplan–Meier, and cox regression survival analysis.
Results
The expression values of 21 lipid metabolism genes showed significant association with different clinical parameters including grade, node, tumor size, stage, race, age, and molecular subtypes of BC patients. Furthermore, survival analysis revealed that ABAT, SRD5A1, and PSAT1 were significantly associated with RFS, DFS, and OS in BC. Overexpression of ABAT was significantly associated with better RFS, DFS, and OS in all independent cohorts. In contrast, overexpression of SRD5A1 and PSAT1 was found associated with poor RFS, DFS, and OS. According to in vitro validation results, mRNA expression of ABAT was downregulated, while, SRD5A1 and PSAT1 were upregulated in all tested BC cell lines compared to normal cells.
Conclusion
We concluded that ABAT can be used as a better prognostic marker, while SRD5A1 and PSAT1 can be used as poor prognostic markers in breast cancer patients. These findings can lead us to devise new strategies classifying BC into different prognosis groups which may lead to novel treatment approaches.
The quantitative importance of key root traits on radial water loss
Authors: Song Zhiwei , Zonta Francesco , Ogorek Lucas León Peralta , Bastegaard Viggo Klint , Herzog Max , Pellegrini Elisa , Pedersen Ole 
Published: July 2022

Abstract
AIMS: Root tissue water can be lost to the dry topsoil via radial water loss (RWL) resulting in root shrinking and loss of contact with the rhizosphere. The root barrier to radial oxygen loss (ROL) has been shown to restrict RWL, therefore we hypothesized that the inducible barrier can be formed as a response to low soil water potential and play a role, together with other root traits, in restricting RWL.METHODS: Rice and wheat were grown in hydroponics with contrasting water potential to diagnose ROL barrier formation and to explore how key root traits (ROL barrier, root diameter, root porosity) affect RWL. Moreover, we developed a numerical model predicting RWL as a function of root diameter, root porosity and presence of a barrier to ROL.RESULTS: Methylene blue staining showed that low water potential induced a ROL barrier formation in roots of rice, and also resulted in an apoplastic barrier, as identified by the apoplastic tracer periodic acid. The barrier significantly restricted RWL, but root diameter and tissue porosity also influenced RWL. Our numerical model was able to reflect the empirical data and clearly demonstrated that thick roots and a barrier to ROL restricts RWL while cortical porosity accelerates RWL.CONCLUSIONS: Our modelling approach highlighted that increase in root tissue porosity, a common response to drought, conserves water when new roots are formed, but the higher desiccation risk related to high-porosity roots can be effectively counteracted by forming thick roots or even better, by a barrier to ROL.
Analysis of the curative effect of arthroscopic coracoid process drill-free U-shaped suspension fixation in the treatment of acute acromioclavicular joint dislocation
Authors: Liu Yongsheng , Yang Yuhang , Zhao Daohong 
Published: July 2022

Abstract
Background: Currently, there is still have some controversies in surgical treatment modality for acute acromioclavicular joint dislocation. The objective of this study is to investigate the clinical effect of arthroscopic coracoid process drill-free U-shaped suspension fixation in the treatment of acute acromioclavicular joint dislocation, compared with coracoid process drilling TightRope fixation technique.Patients and methods: Twenty-five patients with acute acromioclavicular joint dislocation admitted to our hospital were reviewed from May 2015 to June 2021. The patients were divided into drill-free group (the use of coracoid process drill-free U-shaped suspension fixation technique under arthroscopy, 12 cases) and drilling group (the use of coracoid process drilling TightRope fixation technique under arthroscopy, 13 cases). The (UCLA) score, (VAS) score, Constant score and shortest distance between the coracoid process and clavicle (CCD) were evaluated before and after surgery. Results: The patients were followed up with a mean time of 41.3 (rang,12-84) months. At the final follow-up, the postoperative (UCLA) score, Constant score and (VAS) score of both groups were significantly improved compared with those before surgery, and the difference was significant (P<0.05). In terms of imaging evaluation, the (CCD) and the side-to-side (CCD) difference of both groups were significantly reduced immediately after surgery and at the final follow-up, and the difference were significant (P<0.05). There was no significant difference between the groups. Unfortunately, reduction loss occurred in 4 patients (drill-free group: 33%, drilling group: 31%) in both groups, but it had no significant effect on patients' pain perception and life and work, 2 patients (15%) in the drilling group developed coracoid fractures in the postoperative 6-week follow-up and received surgery, no cases of coracoid fractures were observed in the drill-free group.Conclusion: The use of arthroscopic coracoid process drill-free U-shaped suspension fixation in the treatment of patients with acute acromioclavicular joint dislocation can obtain the same good clinical effects as the arthroscopic coracoid process drilling TightRope technique, and can reduce the complications of coracoid fractures.Level of evidence: Level IV, therapeutic case series.
A Novel Lynch Syndrome Kindred with Hereditary Adrenal Cortical Carcinoma
Authors: Ahuja Kripa , Goudar Ranjit 
Published: July 2022

Abstract
Background: Adrenal cortical carcinoma (ACC) is an extremely rare malignancy, and advanced ACC carries a very poor prognosis. Early detection is critical, since early-stage disease can be cured with surgical resection. ACC can be seen in Lynch syndrome; this case and review of the literature provides insight as to the potential biological origin of this malignancy. Clinicians should be aware of this association and the potential impact on cancer screening in these kindreds. Case Presentation: We describe a novel kindred with hereditary adrenal cortical carcinoma and the Muir-Torre syndrome, a phenotypic variant of Lynch syndrome that includes sebaceous neoplasms and visceral malignancies. We report a 59-year-old Caucasian man with an MSH2 deletion who was diagnosed with metastatic adrenal cortical carcinoma. The patient’s brother also had a history of adrenal cortical carcinoma. The patient’s cancer initially responded to immunotherapy with pembrolizumab. Somatic genetic testing performed on a tumor biopsy did not identify the germline MSH2 deletion.Conclusions: Review of the literature identifies an association between germline MSH2 mutations and ACC, suggesting a potential biologic basis for carcinogenesis. This case highlights the importance of ACC screening for patients with Lynch Syndrome and a family history of adrenal cortical carcinoma, due to the high mortality from this malignancy. This case also highlights the importance of separate germline and somatic testing for patients with a concerning personal or family history of cancers.
Spatiotemporal variation and multivariate controls of short-cycle drought-flood abrupt alteration in China’s north-south transitional zone
Authors: lei xiaoping , ma rui , song xiaoyan , hou jingzhi , liu wenbin 
Published: July 2022

Abstract
A full knowledge of the alteration patterns of drought and flood in adjacent months is crucial to facilitate climate adaptation and mitigation strategies. However, the exploring of short-cycle drought-flood abrupt alteration (s-DFAA) and its multi-scale responses to multiple environmental factors remains poorly understood due to insufficient formula construction (e.g., drought standards cannot be introduced) of the existing short-cycle drought-flood abrupt alternation index (SDFAI). In this study, we proposed a new short-cycle drought-wetness abrupt alternation index (SDWAI) allowing to customize drought standards and applied which to China’s north-south transition zone. The multi-scale relationships (i.e. small scales (<4 years), medium scales (4-8 years), large scales (>8years) and all scales) between the s-DFAA(SDWAI) and its pertinent factors (i.e., precipitation, relative humidity, sunshine duration, temperature, evaporation; Arctic Oscillation, AO; NINO 3.4 SSTA Index, NINO3.4; Total Sunspot Number Index, TSNI; Pacific Decadal Oscillation Index, PDO) were further investigated using the wavelet transform coherence and multiple wavelet coherence. Results showed that the SDWAI outperformed the traditional one. Different-level s-DFAA events(i.e., mild, moderate, severe, extreme and total) identified by SDWAI exhibited insignificant downward trends. Spatially, there were more s-DFAA events in the east than the west. Among the single and multiple meteorological elements, precipitaion was the dominant factor influencing the SDWAI, with the highest percent area of significant coherence (PASC) at different scales. Each teleconnection and their combinations, which were irreplaceable, had the most siginificant influence on SDWAI at large scales where TSNI and NINO3.4 that contributed higher PASC were substantiated the predominant roles.
Umbilical Mesenchymal Stem Cell-derived Exosomes Regulate CD4+ T cells in Patients with Primary Sjogren's Syndrome through the Autophagy Pathway
Authors: Ma Dan , Su Yazhen , Wu Zewen , Zhao Xingxing , Zhu Xueqing , An Qi , Yang Baoqi , Wang Yajing , 赵 Jingwen , Xu Ke , Zhang Liyun 
Published: July 2022

Abstract
Background: Primary Sjögren's syndrome (pSS) is an autoimmune disease that leads to the destruction of exocrine glands and formation of multisystem lesions. Abnormal proliferation, apoptosis, and differentiation of CD4+ T cells are key factors in the pathogenesis of pSS, seriously affecting the appearance, progression, and outcome of the disease. Human umbilical cord mesenchymal stem cell-derived exosomes (UCMSC-Exos) may effectively simulate the immunoregulatory and tissue repair functions of MSCs while avoiding the risks associated with MSCs treatment. Hence, UCMSC-Exos treatment is expected to become a new treatment for pSS. However, if UCMSC-Exos can regulate the abnormal proliferation, apoptosis, and differentiation of CD4+ T cells in pSS and the specific underlying mechanisms remain to be elucidated. Methods: UCMSC-Exos were isolated and identified. Peripheral blood CD4+ T-cells were sorted using immunomagnetic beads. The proliferation, apoptosis, differentiation, and inflammatory factors of CD4+ T cells were determined using flow cytometry. Autophagosomes of CD4+ T cells were detected using transmission electron microscopy, autophagy-related proteins were detected using western blotting, and autophagy-related genes were detected using RT-PCR. Results: UCMSC-Exos inhibited excessive proliferation of peripheral blood CD4+ T cells in pSS patients, blocked the G0/G1 phase transition, inhibited S phase cells, suppressed excessive apoptosis of CD4+ T cells, reduced the Th17 cell ratio, elevated the regulatory T cell (Treg) ratio, restored the Th1/Th2 and Th17/Treg balance, inhibited the CD4+ T cell-associated pro-inflammatory factors IFN-γ, TNF-α, IL-6, IL-17A, and IL-17F secretion, and promoted anti-inflammatory factor IL-10 and TGF-β secretion. UCMSC-Exos reduced the elevated autophagy levels in the peripheral blood CD4+ T cells of patients with pSS. Furthermore, UCMSC-Exos regulated CD4+ T cell proliferation and early apoptosis in pSS patients through the autophagy pathway, inhibited Th17 cell differentiation, promoted Treg cell differentiation, and restored the Th17/Treg balance. Conclusions: UCMSC-Exos regulate CD4+ T cell proliferation, apoptosis, and differentiation in patients with pSS through the autophagy pathway and exhibit immunoregulatory effects. These results provide an important theoretical and experimental foundation for exploring UCMSC-Exos as a new treatment for pSS and its application in clinical transformation.
Evaluation of the focus reduction neutralization and ELISA tests compared to the plaque reduction neutralization test for the detection of antibodies against measles virus
Authors: yaghoobizad somayeh , Norouzbabaei Zahra , Jandaghi Nazanin Zahra Shafiei , Foroushani Abbas Rahimi , Sadeghi Kaveh , Izadi Shahrokh , Heidari Elaheh , Salimi Vahid , Mokhtari-Azad Talat 
Published: July 2022

Abstract
Measles is an infectious febrile sickness. Despite the existence of effective vaccination, eradicating measles remains a global priority. It is required to set up a sensitive and specific test to identify measles-specific antibodies in order to estimate the level of community protection against measles. Although the plaque reduction neutralization test (PRNT), the gold standard test, is technically challenging, it is not suitable for routine use. The enzyme-linked immunosorbent assay (ELISA) was also unable to detect weak antibody responses in people vaccinated against measles. As a result, the FRNT (focus reduction neutralization test) was established and validated. In eight assay runs, the samples were analyzed in triplicate by PRNT, FRNT, and ELISA. In a total of 50 samples analyzed, 38/50 samples were positive by standard PRNT, 37/50 samples by FRNT, and 19/50 samples were positive by ELISA. The results showed that ELISA was not sensitive enough to identify low levels of anti-measles antibodies, and that ELISA and both neutralization assays had a weak agreement. The two neutralization assays, on the other hand, had a perfect correlation. Finally, the PRNT and FRNT have similar sensitivity. For characterizing the immunological response to measles and vaccination efficacy trials, FRNT seems to be a good alternative to PRNT. Therefore, it will be very important to validate the negative and equivocal ELISA results with this standard test in the final stages of eradication.
Correlation of Dynamic Contrast-Enhanced MR Imaging (DCE-MRI) and Histopathology in Osteosarcoma Response to Neoadjuvant Chemotherapy
Authors: Johan Muhammad Phetrus , Saleh M. Ruksal , Ariandi Mirza 
Published: July 2022

Abstract
Background To evaluate the dynamic contrast-enhanced MRI (DCE-MRI) parameters for monitoring the neoadjuvant chemotherapy (NAC) response in osteosarcoma and correlate the parameters of histopathology specimens.Methods Patients with pathologically confirmed as osteosarcoma had three cycles of NAC administered. Dynamic MRI were performed on all patients before and after chemotherapy. Semi-quantitative and quantitative parameters were compared between the good response group (TNR ≥ 90 percent) and poor response group (TNR < 90 percent) using the histology response as the reference standard (tumor necrosis rate). The Mann-Whitney U test was used to assess the differences in DCE-MRI parameters before and after NAC. Results 15 patients were enrolled in this study of whom 3 patients lack of preoperative imaging examination, 2 patients inoperable to asess tumor necrosis rate and 1 had an allergy to a contrast agent. DCE-MRI of n = 9 patients were subsequently used for this study. Prior to chemotherapy, there were no statistically significant differences between the good response group and poor response group in TTP, AUC, MAX Slope, Ktrans, Kep, and Ve (P < 0.05). Following the completion of chemotherapy, the TTP, AUC, MAX Slope, Kep and Ktrans were significantly difference between the good response group and poor response group (P < 0.05). The Ve did not show significant difference between the two groups after the chemotherapy (P = 0,121).Conclusion DCE-MRI has effectively monitor the response to NAC during osteosarcoma treatment cycle.
Non-invasive early prediction of Immune Checkpoint Inhibitor efficacy in Non-Small Cell Lung Cancer patients using on-treatment serum CRP and NLR
Authors: Matsuzawa Reiko , Morise Masahiro , Kinoshita Fumie , Tanaka Ichidai , Koyama Junji , Kimura Tomoki , Kondoh Yasuhiro , Tanaka Taro , Shima Koichiro , Hase Tetsunari , Wakahara Keiko , Ishii Makoto , Hashimoto Naozumi 
Published: July 2022

Abstract
PurposeWe determined the clinical relevance of early C-reactive protein (CRP) and neutrophil-lymphocyte ratio (NLR) change in blood as surrogate markers of pro-tumor inflammation (PTI) for predicting clinical outcome of programmed cell death (PD)-1/programmed cell death ligand (PD-L) 1 inhibitor treatment in non-small cell lung carcinoma (NSCLC).MethodsWe retrospectively reviewed NSCLC patients treated with anti-PD-1 or PD-L1 inhibitors. Early CRP change was defined as the ratio of 6-week CRP to baseline CRP, and early NLR change was defined as that of the 6-week NLR to baseline NLR. PTI index was determined by combinatorial evaluation of early CRP change and early NLR change, PTI index low: both of these were low, intermediate: either of these was low, high: both of these were high.ResultsThe study included 217 patients. Early CRP change and early NLR change were both associated with PFS and OS. The combinatorial evaluation using these two markers enabled the clear stratification of PFS and OS. The median PFS in patient with PTI index low was 13.9 months, while the median PFS in those with PTI index high was 2.5 months (p < 0.01, log-rank test). The median OS in patients with PTI index low was not reached; the median OS in those with PTI index high was only 15.4 months (p < 0.01, log-rank test).ConclusionsThe combinatorial early CRP change and early NLR change as PTI biomarkers have clinical potential in identifying NSCLC patients who can achieve a durable response and long-term survival using PD-1/PD-L1 inhibitors.