bluuebunny/crossref_metadata_2025_split huggingface dataset:
Greater breadth of vaccine-induced immunity in females than males is mediated by increased antibody diversity in germinal center B cells
Authors: Ursin Rebecca L. , Dhakal Santosh , Liu Hsuan , Jayaraman Sahana , Park Han-Sol , Powell Harrison R. , Sherer Morgan L. , Littlefield Kirsten E. , Fink Ashley L. , Ma Zexu , Mueller Alice L. , Chen Allison P. , Woldetsadik Yishak A. , Gearhart Patricia J. , Larman H. Benjamin , Maul Robert W. , Pekosz Andrew , Klein Sabra L. 
Published: June 2022

AbstractInactivated influenza vaccines induce greater antibody responses in females than males among both humans and mice. To test the breadth of protection, we used recombinant mouse-adapted A/California/2009 (maA/Cal/09) H1N1 viruses containing mutations at one (1M), two (2M), or three (3M) antigenic sites, in addition to a virus containing the 1M mutation and a substitution of the Ca2 antigenic site (Sub) with one derived from an H5 hemagglutinin (HA) to challenge mice of both sexes. Following maA/Cal/09 vaccination, females produced greater virus-specific class-switched IgG and IgG2c antibodies against the vaccine and all mutant viruses, and antibodies from females recognized more unique, linear HA epitopes than antibodies from males. While females had greater neutralizing antibody (nAb) titers against the vaccine virus, both sexes showed lower neutralization capacity against mutant viruses. After virus challenge, vaccinated females had lower pulmonary virus titers and reduced morbidity than males against the 1M and 2M viruses, but not the Sub virus. Females generated greater numbers of germinal center (GC) B cells containing superior somatic hypermutation frequencies than vaccinated males. Deletion of activation-induced cytidine deaminase (Aicda) eliminated female-biased immunity and protection against the 2M virus. Harnessing methods to improve GC B cell responses and frequencies of somatic hypermutations, especially in males, should be considered in the development of universal influenza vaccines.SummaryCompared with males, inactivated influenza vaccination of female mice causes greater production of class-switched, somatically-hypermutated antibodies and a more robust germinal center B cell response, leading to more diverse H1N1 antigen recognition and better protection against mutant influenza A viruses.
Ban on wild bird importation accelerated the spread of global viral outbreaks in parrots
Authors: Komorizono Ryo , Aihara Naoyuki , Fujiwara Chinatsu , Kamiie Junichi , Tomonaga Keizo , Makino Akiko 
Published: June 2022

AbstractSince the isolation of the first parrot bornavirus (PaBV), which causes slow-onset, fatal neurological disease in various parrot species, in the United States in 2008, PaBVs have spread rapidly worldwide; however, the reason remains unknown. In this study, we show that the most dominant lethal genotype, PaBV-4, spread via the global trade of captive birds. Analyses of traded parrot numbers in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) database and PaBV-4 phylodynamics suggested that the ban of wild imported birds in the European Union (EU) in 2007 facilitated the international trade of captive parrots, which resulted in an increase in the effective population size of PaBV-4. This change coincided with a historical PaBV-4 epidemic. These data suggest that due to the low transmission efficiency and long incubation period of PaBV-4, the majority of PaBV-4 transmission occurred in breeding facilities and the increased trade of captive parrots accelerated the global spread of PaBV-4 infection. Our results indicate that interventions for the protection of wild animals and prevention of infectious diseases may conversely cause epidemics of infectious diseases in the global system. Conservation of ecosystems requires not only the establishment of importation restrictions and maintenance of the diversity of wild animals but also the implementation of multifaceted management measures, such as quarantine policies and breeding control in captive animals.
Assessing emergence risk of double-resistant and triple-resistant genotypes of Plasmodium falciparum
Authors: Li Eric Zhewen , Nguyen Tran Dang , Tran Thu Nguyen-Anh , Zupko Robert J , Boni Maciej F 
Published: June 2022

AbstractDelaying and slowing antimalarial drug resistance evolution is a priority for the World Health Organization and for National Malaria Control Programs in malaria-endemic countries. Until novel therapies become available, the mainstay of antimalarial treatment will continue to be artemisinin combination therapy (ACT), with artemether-lumefantrine, artesunate-amodiaquine, and dihydroartemisinin-piperaquine the three primary therapies deployed worldwide. Deployment of ACTs can be optimized to minimize evolutionary pressure for drug resistance by deploying them as a set of co-equal multiple first-line therapies (MFT) rather than rotating therapies in and out of use. Here, we consider one potential detriment of MFT policies, namely, that the simultaneous deployment of multiple ACTs could drive the evolution of different resistance alleles concurrently and that these resistance alleles could then be brought together by recombination into double-resistant or triple-resistant parasites. Using an individual-based Plasmodium falciparum transmission model, we compare MFT and cycling policies over 20-year periods in malaria transmission settings ranging from 0.1% to 25% Plasmodium falciparum prevalence (PfPR2-10). We define a total risk measure for multi-drug resistance (MDR) by summing the area under the genotype-frequency curves (AUC) of double- and triple-resistant genotypes. When PfPR ≥ 1%, total MDR risk as measured by AUC is 4% to 90% lower under MFT policies than under cycling policies, irrespective of whether resistance is imported or emerges de novo. When PfPR = 0.1%, there is little statistical difference in MDR risk between MFT and cycling.
Aberrant cortical spine dynamics after concussive injury are reversed by integrated stress response inhibition
Authors: Frias Elma S. , Hoseini Mahmood S. , Krukowski Karen , Paladini Maria Serena , Grue Katherine , Ureta Gonzalo , Rienecker Kira D.A. , Walter Peter , Stryker Michael P. , Rosi Susanna 
Published: June 2022

AbstractTraumatic brain injury (TBI) is a leading cause of long-term neurological disability in the world and the strongest environmental risk factor for the development of dementia. Even mild TBI (resulting from concussive injuries) is associated with a >2-fold increase in the risk of dementia onset. Little is known about the cellular mechanisms responsible for the progression of long lasting cognitive deficits. The integrated stress response (ISR), a phylogenetically conserved pathway involved in the cellular response to stress, is activated after TBI, axsnd inhibition of the ISR — even weeks after injury — can reverse behavioral and cognitive deficits. However, the cellular mechanisms by which ISR inhibition restores cognition are unknown. Here we used longitudinal two-photon imaging in vivo after concussive injury in mice to study dendritic spine dynamics in the parietal cortex, a brain region involved in working memory. Concussive injury profoundly altered spine dynamics measured up to a month after injury. Strikingly, brief pharmacological treatment with the drug-like small-molecule ISR inhibitor ISRIB entirely reversed the structural changes measured in the parietal cortex and the associated working memory deficits. Thus, both neural and cognitive consequences of concussive injury are mediated in part by activation of the ISR and can be corrected by its inhibition. These findings suggest that targeting ISR activation could serve as a promising approach for the clinical treatment of chronic cognitive deficits after TBI.Significance StatementAfter traumatic brain injury, temporary pharmacological inhibition of the integrated stress response (ISR), with a small-molecule inhibitor (ISRIB), rescued long lasting trauma-induced cognitive deficits. Here, we found that ISRIB treatment rapidly and persistently reversed the aberrant changes in cortical spine dynamics in the parietal cortex while rescuing working memory deficits. These data suggests that the link between the ISR and memory function involves, at least in part, changes in neuronal structure. Targeting ISR activation could serve as a promising approach for the clinical treatment of chronic cognitive deficits after brain injuries.
Atomistic simulation of protein evolution reveals sequence covariation and time-dependent fluctuations of site-specific substitution rates
Authors: Norn Christoffer , André Ingemar 
Published: June 2022

AbstractThermodynamic stability is a crucial fitness constraint in protein evolution and is a central factor in shaping the sequence landscapes of proteins. The correlation between stability and molecular fitness depends on the mechanism that relates the biophysical property with biological function. In the simplest case, stability and fitness are related by the amount of folded protein. However, when proteins are toxic in the unfolded state, the fitness function shifts, resulting in higher stability under mutation-selection balance. Likewise, a higher population size results in a similar change in protein stability, as it magnifies the effect of the selection pressure in evolutionary dynamics. This study investigates how such factors affect the evolution of protein stability, site-specific mutation rates, and residue-residue covariation. To simulate evolutionary trajectories with realistic modeling of protein energetics, we develop an all-atom simulator of protein evolution, RosettaEvolve. By evolving proteins under different fitness functions, we can study how the fitness function affects the distribution of proposed and accepted mutations, site-specific rates, and the prevalence of correlated amino acid substitutions. We demonstrate that fitness pressure affects the proposal distribution of mutational effects, that changes in stability can largely explain variations in site-specific substitution rates in evolutionary trajectories, and that increased fitness pressure results in a stronger covariation signal. Our results give mechanistic insight into the evolutionary consequences of variation in protein stability and provide a basis to rationalize the strong covariation signal observed in natural sequence alignments.
Non-rapid eye movement sleep determines resilience to social stress
Authors: Bush Brittany J. , Donnay Caroline , Andrews Eva-Jeneé A. , Lewis-Sanders Darielle , Gray Cloe L. , Qiao Zhimei , Brager Allison J. , Johnson Hadiya , Brewer Hamadi C.S , Sood Sahil , Saafir Talib , Benveniste Morris , Paul Ketema N. , Ehlen J. Christopher 
Published: June 2022

AbstractResilience, the ability to overcome stressful conditions, is found in most mammals and varies significantly among individuals. A lack of resilience can lead to the development of neuropsychiatric and sleep disorders, often within the same individual. Despite extensive research into the brain mechanisms causing maladaptive behavioral-responses to stress, it is not clear why some individuals exhibit resilience. To examine if sleep has a determinative role in maladaptive behavioral-response to social stress, we investigated individual variations in resilience using a social-defeat model for male mice. Our results reveal a direct, causal relationship between sleep amount and resilience—demonstrating that sleep increases after social-defeat stress only occur in resilient mice. Further, we found that within the prefrontal cortex, a regulator of maladaptive responses to stress, pre-existing differences in sleep regulation predict resilience. Overall, these results demonstrate that increased NREM sleep, mediated cortically, is an active response to social-defeat stress that is both necessary and sufficient for promoting resilience. They also show that differences in resilience are strongly correlated with inter-individual variability in sleep regulation.
Connectomic analysis of thalamus-driven disinhibition in cortical layer 4
Authors: Hua Yunfeng , Loomba Sahil , Pawlak Verena , Laserstein Philip , Boergens Kevin M. , Kerr Jason N. D. , Helmstaedter Moritz 
Published: June 2022

ABSTRACTIn mammals, sensory signals are transmitted via the thalamus primarily to layer 4 of the primary sensory cortices. While information about average neuronal connectivity in this layer is available, the detailed and higher-order circuit structure is not known. Here, we used 3-dimensional electron microscopy for a connectomic analysis of the thalamus-driven inhibitory network in a layer 4 barrel. We find that thalamic input drives a subset of interneurons with high specificity. These interneurons in turn target spiny stellate and star pyramidal excitatory neurons with subtype specificity. In addition, they create a directed disinhibitory network directly driven by the thalamic input. Together, this circuit can create differential windows of opportunity for activation of the types of excitatory neurons in dependence of strength and timing of thalamic input. With this, we have identified a so-far unknown degree of specialization of the microcircuitry in the main thalamocortical recipient layer of the primary sensory cortex.
Economic complexity and entrepreneurship: insights from Africa
Authors: Ajide Folorunsho M. 
Published: June 2022

Purpose
Economic complexity reflects the nature of knowledge accumulated and technological capability of a nation. This study aims to evaluate the impact of economic complexity on entrepreneurship in selected African countries.

Design/methodology/approach
The study uses country’s level data of 18 countries covering a period of 2006–2017. Data are sourced from Massachusetts Institute of Technology’s Observatory of Economic Complexity database, World Bank’s Entrepreneurship Database and World Development indicators. To estimate models, the study uses panel-spatial correlation consistent, which is based on Driscoll and Kraay’s (1998) standard error, Method of Moments Panel Quantile regression proposed by Machado and Silva (2019) and instrumental variables estimation techniques.

Findings
The study’s findings are as follows. First, economic complexity improves entrepreneurship in Africa. Second, there is no evidence of nonlinear relationship between economic complexity and entrepreneurship for the case of African nations. The positive impact of economic complexity on entrepreneurship is persistent across all quantiles in the analysis. The empirical analysis suggests that the beneficial impact of African entrepreneurship is further strengthened by ethnic and religious diversity but reduced by weak political institutions.

Originality/value
This study stresses the role of economic complexity in the entrepreneurial activities. To the best of the authors’ knowledge, this is the first attempt to empirically provide insights on the important role of economic complexity on entrepreneurship in Africa.
Green innovation; a way to enhance economic performance of Chinese hotels
Authors: Gu Shengyu 
Published: June 2022

Purpose
This study aims to examine the influencing factors of green innovation and their effect on economic performance of the hotel industry.

Design/methodology/approach
The study used a quantitative and cross-sectional research design, and primary data were collected using structured questionnaire. To analyze this data, AMOS software was used. Exploratory factor analysis (EFA) was performed to purify the measurement model. Moreover, validities and model fitness were confirmed through confirmatory factor analysis (CFA). Structural equation modeling (SEM) was used to test the study hypothesis.

Findings
The study results showed green organizational culture, green organizational strategies, energy conservations and environmental regulations as key influencing factors in the adoption of green innovation. Moreover, the results also reveal a significant positive effect of green innovation on hotels’ economic performance.

Research limitations/implications
This study is limited to hotels operating in the sole city of Shanghai, China, and for more generalized results, the future study can be made by incorporating hotels in other cities as well. Moreover, the study is conducted in China and replication of this research might be possible in a different cultural and environmental context. Furthermore, this study address only the hotel industry, and future study can be made to investigate the implementation of green practices in industries other than hotels.

Originality/value
Previously, many studies have highlighted the influencing factors of green innovation, but there is limited research in the context of the hotel industry. The relationship between economic performance and green innovation is well established, but still, it is not fully understood how sustainable economic performance is contributed by green innovation in the tourism industry. To the best of the authors’ knowledge, this study first examines the influencing factors of green innovation and its potential effect on economic performance in the context of the Chinese hotel industry.
Non-Newtonian couple stress lubrication of MHD elastic journal bearings
Authors: Kardoudi Mohammed , Nabhani Mohamed , El Khlifi Mohamed 
Published: June 2022

Purpose
This paper aims to present a numerical model to examine the finite magneto-hydrodynamic (MHD) journal bearings performances including both non-Newtonian couple stress and bearing deformation impacts.

Design/methodology/approach
Based upon the MHD and Stokes theories, a novel expression of modified Reynolds equation including bearing deformation is obtained. The bearing elastic deformation impact is predicted by means of the Winkler model. Using the numerical differentiation approach, the film pressure is iteratively solved. Different bearing characteristics are then numerically calculated. The validity of the proposed model was verified by comparing with some particular cases from literature.

Findings
From the numerical presented results, it is demonstrated that the conducting couple stress lubricant with an applied radial magnetic field results in an induced electric current density and thus significantly improves the performances of elastic journal bearings. Particularly, the load-carrying capacity is increased, whereas a reduction in friction factor is observed.

Originality/value
This numerical model is original, which combines both non-Newtonian couple stress and bearing deformation impacts on finite MHD journal bearings performances. It provides useful information in designing MHD journal bearings, given the lack of experimental studies.
Study on the continuance intention in using virtual shoe-try-on function in mobile online shopping
Authors: Jiang Qianling , Gu Chao , Feng Yan , Wei Wei , Tsai Wang-Chin 
Published: June 2022

PurposeMobile e-commerce has brought convenience to consumers. But for goods such as shoes, mobile e-commerce has failed to provide the same experience as consumers would have in physical stores, and that also causes problems for online merchants, such as high return rates. As a result, the augmented reality (AR) virtual shoe-try-on function appeared. The way that AR virtual shoe-try-on study different from other AR virtual try-on studies is that AR virtual shoe-try-on study only satisfies consumers' visual experience and consumers cannot judge whether the shoes are comfort or not. Whether consumers would accept AR virtual try-on function to help them make purchase decision due to the visual experience provided by AR virtual try-on function is worth discussion. Measuring users' perceptions and preferences can help companies design AR shoe-trying functions and provide services more cost-effectively.Design/methodology/approachTo promote the continuous use and better development of such mobile e-commerce based on the technology acceptance model (TAM), this study explored the influencing factors for users' intentions to continue using the AR virtual shoe-try-on function, including the perceived usefulness, perceived ease of use, system quality, perceived playfulness and attitude.FindingsThe results of this study showed that TAM is a powerful theoretical tool of the new technology in mobile e-commerce and that the system quality and perceived playfulness also have a positive impact on the original variables of TAM. System quality and perceived playfulness are important predictors of users' continuance intentions to use the AR virtual shoe-try-on function.Originality/valueThe main contribution of this study to model iteration and theoretical update is to verify the applicability of the TAM in the AR shoe-try-on function and to expand TAM model with system quality and perceived playfulness. The authors' results will help shoe enterprises win users' recognition through AR shoe-try-on function and improve users' continuance intention of use.
Harmonious passion, effectuation and firm performance: the moderated mediation effect of entrepreneurial experience
Authors: Laskovaia Anastasiia , Lee Younggeun , Bogatyreva Karina , Herrmann Pol 
Published: June 2022

PurposeEntrepreneurial passion lies at the foundation of the business development process, yet the available evidence is ambiguous regarding its effects on firm performance. In this study, we examine the entrepreneurial passion puzzle by looking into decision-making mechanisms underlying its potential to increase business performance outcomes. Based on the literature on passion and effectuation theory, we link harmonious passion to firm performance through effectual behavioral logic.Design/methodology/approachTo empirically test the theoretical model, we performed bootstrapping-based mediation, ordinary least squares moderated regression and moderated mediation analysis based on survey data collected from 105 executives of US firms.FindingsWe found that harmonious passion indirectly increases firm performance through effectuation. The direct link between harmonious passion and firm performance is insignificant. Moreover, the proposed mediation model is strengthened when the executives attain high levels of entrepreneurial experience.Research limitations/implicationsThis study is not without limitations. First, a cross-sectional data set was adopted as the empirical setting of our research. Hence, further studies could benefit from applying longitudinal research designs. Second, a self-reported survey was utilized to measure firm performance. Although such operationalization is widely accepted in management and entrepreneurship studies, objective measures can produce additional insights into focal relationships.Practical implicationsThis study provides practical implications for educators, consultants and managers. First, educators should encourage harmonious passion and teach effectual decision-making through various educational programs. Consultants and business practitioners should take similar orientations when organizing training for employees and executives. Finally, managers need to comprehend the motivational types of their employees and promote harmonious passion through appraisal systems and organizational culture.Originality/valueThis research uncovers a mechanism and a boundary condition on the relationship between harmonious passion and performance. The results show the critical moderating role of human capital and the mediating role of effectual decision-making on the passion and performance relationship. The current study contributes to the passion literature by providing a more nuanced understanding of the dynamics between entrepreneurial passion and firm performance.