bluuebunny/crossref_metadata_2025_split huggingface dataset:
LBODP078 Role Of Ire1a-xbp1 Pathway In Rodent Functional Pituitary Tumor Cells.
Authors: Nezu Masahiro , Satou Motoyasu , Nakano-Tateno Tae , Chik Constance L , Tateno Toru 
Published: November 2022

Abstract

Introduction
Pituitary tumors (PTs) occur in almost 17% of the population and they represent about 10% of all intracranial tumors. At present, many patients with pituitary tumors suffer from symptoms and complications caused by abnormal hormone production and/or mass effects due to limited treatment options. Therefore, the development of novel therapies is an urgent issue. The inositol requiring enzyme 1a (IRE1a)-X-box binding protein 1 (XBP1) pathway is an important component for the endoplasmic reticulum (ER) stress response. This pathway is activated by immature protein overload in the ER and elimination or repair of such protein can reduce ER stress. This pathway has been reported to regulate cancer cell growth and secretory protein production. The detection of potential XBP1 binding sites in the promoter region of rat GH and mouse POMC similar to those of human genes suggests the involvement of the IRE1a-XBP1 pathway in the production of these hormones and pituitary tumor cell growth. In this study, we aimed to clarify the role of the IRE1a-XBP1 pathway in functional pituitary tumor cells.

Methods and Results
We conducted in vitro experiments by using rat GH-producing pituitary GH4 cells and mouse ACTH-producing AtT-20 cells as the models of GH- and ACTH-producing pituitary adenomas, respectively. Cell proliferation was measured by WST-8 assay, gene expression levels by qPCR, and protein expression by western blotting, respectively. In GH4 cells, STF08310 (40µM), an IRE1a inhibitor, reduced levels of GH and XBP1 spliced form (XBP1s) mRNA and GH protein with suppressed cell proliferation. In AtT-20 cells, STF08310 (40µM) also suppressed protein expression of proopiomelanocortin (POMC) and cell proliferation. Gene suppression of IRE1a by siRNA and over-expression of XBP1s showed marked reduction and increase of POMC protein, respectively.

Conclusion
Our findings suggest that the IRE1a-XBP1s pathway would be a possible therapeutic target that has both anti-tumoral and hormone control effects against functional pituitary tumor.
Presentation: No date and time listed
ODP288 A Cautionary Tale of Two Endocrine Emergencies- A Patient with Pituitary Macroadenoma Presenting with Acute Adrenal Crisis Leading to Pituitary Apoplexy
Authors: Khalifa Maram , Faisal Sobia , Kantorovich Vitaly 
Published: November 2022

Abstract

A 56 year old male with history significant for myasthenia gravis, non-functional pituitary macroadenoma and age related macular degeneration (ARMD), who presented to the hospital with 3 days of diplopia, nausea, vomiting, generalized weakness, and bitemporal headaches. Two months prior to presentation, he was diagnosed with an idiopathic disseminated rash for which he was treated with high dose steroids. Steroids were tapered rapidly and stopped abruptly 4 days ago. Initial presentation was significant for hypotension with BP of 95/64, hyponatremia [sodium 127(136 - 145 mmol/L)], chloride was 90 (98 - 107 mmol/L), potassium 5.9 (3.4 - 5.3 mmol/L) consistent with acute adrenal crisis. He was immediately initiated on high dose Solu-Cortef and intravenous fluids. Of historical significance was new visual field deficit which had occurred in the background of his existing ARMD. This raised the suspicion for pituitary apoplexy. MRI pituitary showed enlarging pituitary adenoma (2.1×2. 0×2.6 cm from 1.6×1.6×2.3 cm 5 months prior) with necrotic changes and internal signal complexity. Ophthalmology was consulted stat and on confirmation of new right optic neuropathy, urgent surgical decompression was performed via transnasal endoscopic resection of pituitary tumor by neurosurgical and ENT team. Further lab testing revealed secondary adrenal insufficiency and pan hypopituitarism. Patient's vision recovered remarkably well post operatively and he was initiated on thyroid hormone supplementation in addition to continuing steroids. He was discharged with recommendations to follow up with endocrinology outpatient for further management.

Conclusion
Pituitary apoplexy although well known can easily be missed clinically without a high degree of suspicion. While our patient presented with acute adrenal crisis leading to some of the presenting complaints, his new visual field deficits were unaccounted for. This case highlights the importance of a through history and examination to delineate between acute and chronic visual field symptoms especially in the background of pre-existing retinal diseases. Secondly, although the patient's adrenal insufficiency could be due to apoplexy, we hypothesize that it is more likely that it could have resulted due to abrupt discontinuation of high dose steroids leading to adrenal crisis. The fluctuation in BP in turn could have triggered an apoplectic event in an otherwise stable macroadenoma leading to two independent but interrelated endocrine emergencies.
Presentation: No date and time listed
Incidence, risk and impact of unplanned ICU readmission on patient outcomes and resource utilisation in tertiary level ICUs in Nepal: A cohort study
Authors: Aryal Diptesh , Paneru Hem Raj , Koirala Sabin , Khanal Sushil , Acharya Subhash Prasad , Karki Arjun , Dona Dilanthi Gamaga , Haniffa Rashan , Beane Abi , Salluh Jorge I F 
Published: November 2022

Background: Unplanned readmissions to Intensive Care Units (ICUs) result in increased morbidity, mortality, and ICU resource utilisation (e.g. prolonged mechanical ventilation), and as such, is a widely utilised metric of quality of critical care. Most of the evidence on incidence, characteristics, associated risk factors and attributable outcomes of unplanned readmission to ICU are from studies performed in high-income countries This study explores the determinants of risk attributable to unplanned ICU readmission in four ICUs in Kathmandu, Nepal. Methods: The registry-embedded eCRF reported data on case mix, severity of illness, in-ICU interventions (including organ support), ICU outcome, and readmission characteristics. Data were captured in all adult patients admitted between September 2019 and February 2021. Population and ICU encounter characteristics were compared between those with and without readmission. Independent risk factors for readmission were assessed using univariate analysis. Results: In total 2948 patients were included in the study. Absolute unplanned ICU readmission rate was 5.60 % (n=165) for all four ICUs. Median time from ICU discharge to readmission was 3 days (IQR=8,1). Of those readmitted, 29.7% (n=49) were discharged at night following their index admission. ICU mortality was higher following readmission to ICU(p=0.016) and mortality was increased further in patients whose primary index discharge was at night(p= 0.019). Primary diagnosis, age, and use of organ support in the first 24hrs of index admission were all independently attributable risk factors for readmission. Conclusions: Unplanned ICU readmission rates were adversely associated with significantly poorer outcomes, increased ICU resource utilisation. Clinical and organisational characteristics influenced risk of readmission and outcome.
Side effects of treatment with tyrosine kinase inhibitors in patients with chronic myeloid leukemia and the occurrence of anxiety symptoms
Authors: Gibek Katarzyna , Sacha Tomasz , Cyranka Katarzyna 
Published: November 2022

Cel pracyCelem pracy było sprawdzenie czy poszczególne skutki uboczne leczenia inhibitorami kinaz tyrozynowych u pacjentów chorych na przewlekłą białaczkę szpikową mogą sprzyjać występowaniu objawów lękowych. Dodatkowo postanowiono sprawdzić, czy występują zależności pomiędzy wiekiem, płcią oraz czasem trwania leczenia a nasileniem lęku w podziale na występowanie poszczególnych skutków ubocznych.MetodaBadanie zostało przeprowadzone w Poradni Hematologicznej Szpitala Uniwersyteckiego w Krakowie, objęto nim 91 chorych. Do analizy występowania objawów lękowych zastosowano ankietę własną, Kwestionariusz Davida Goldberga GHQ-28 oraz czterowymiarowy Kwestionariusz 4DSQ.WynikiNajczęściej wymienianymi skutkami ubocznymi leczenia były: bóle kości i stawów oraz skurcze i bóle mięśni, zatrzymanie wody oraz męczliwość. Średni czas trwania choroby wyniósł 10 lat. Męczliwość, nudności/niestrawność, częste infekcje, bóle kości i stawów, ból brzucha oraz utrata apetytu były skutkami ubocznymi leczenia IKT, które wpływały na zwiększony poziom objawów lękowych. Wiek, długość trwania leczenia oraz płeć w minimalnym zakresie wpływały na występowanie objawów lękowych.WnioskiPraca wskazuje na duże znaczenie występowania skutków ubocznych leczenia IKT dla pojawiania się objawów lękowych. Praca lekarzy, psychologów oraz firm farmaceutycznych nad zmniejszeniem/ złagodzeniem skutków ubocznych może wpłynąć na lepszą jakość życia tych pacjentów. Dodatkowo, prowadzone od kilku lat próby odstawienia leczenia niektórymi IKT (imatynibem i nilotynibem) u pacjentów uzyskujących głęboką odpowiedź molekularną może sprzyjać poprawie ich stanu psychicznego.
PRODUTOS EDUCACIONAIS DIGITAIS PARA AUXÍLIO EM AULAS REMOTAS DE BIOLOGIA NO CONTEXTO DE (PÓS) PANDEMIA COVID-19
Authors: Amorim Douglas Carvalho de , Costa Cleide Jane de Sá Araújo 
Published: November 2022

A pandemia causada pelo vírus Sars-Cov-2, que provocou a doença Covid-19, promoveu o isolamento social afetando assim diferentes setores sociais, incluindo a Educação. Como prevenção à saúde, as aulas presenciais foram substituídas por aulas remotas emergenciais mediadas por tecnologias digitais de forma síncrona e assíncrona. Neste contexto, este estudo propõe investigar como seis produtos educacionais digitais produzidos no intervalo de tempo de 2013 a 2019 no Programa de Pós-Graduação em Ensino de Ciências e Matemática (PPGECIM) da Universidade Federal de Alagoas (UFAL) podem auxiliar professores no ensino remoto de Biologia no contexto atual de pandemia Covid-19 e pós-pandemia. A hipótese que sustentou o estudo foi que todos os produtos educacionais digitais encontrados de 2013 a 2019 apresentam potencialidades para auxiliar professores de Biologia no ensino remoto de diferentes conteúdos da disciplina, no contexto atual da pandemia e pós-pandemia Covid-2019. A abordagem do estudo foi qualitativa com delineamento exploratório bibliográfico. Os dados foram coletados no site do PPGECIM nas dissertações e produtos digitais encontrados. A análise dos dados ocorreu por meio da técnica análise do conteúdo (AC). Como principais resultados, o estudo revelou que diferentes produtos digitais com diferentes temas podem auxiliar professores de Biologia no enriquecimento de aulas remotas no tempo atual e que, no contexto de pós-pandemia, se evidencia contribuições para aulas híbridas.
Co-Creative Vermittlungsplattformen für die Psychische Gesundheitsversorgung
Authors: Kegel Felix , Greve Maike , Schnell Knut , Stein Miriam , Kolbe Lutz M. 
Published: November 2022

ZusammenfassungIm deutschen Gesundheitswesen nimmt die Versorgung psychischer Störungen eine immer wichtigere Rolle ein. Nicht erst seit dem Beginn der Corona-Pandemie steigt die Zahl der Menschen mit psychischen Erkrankungen deutlich an. Damit gehen sowohl wirtschaftliche als auch persönliche Herausforderungen einher.Dieser Artikel leistet einen Beitrag zur digitalen Transformation im Gesundheitswesen, indem eine konzeptionelle Analyse der Vermittlungsproblematik in der psychischen Gesundheitsversorgung vorgenommen wird. Die Studie zeigt, dass digitale Plattformen die Möglichkeit bieten, den bisherigen undurchsichtigen Angebotsmarkt zu strukturieren, Betroffenen sowie deren Angehörigen passende online und offline Versorgungs- und Unterstützungsangebote niederschwellig zugänglich zu machen und die Patient*innenreise sinnvoll zu erweitern. Die Ergebnisse verdeutlichen die Notwendigkeit der Berücksichtigung der Eigenschaften Regionalität, Inklusion und Kollaboration der Plattform sowie die der Umsetzung mit Hilfe co-creativer Methodiken. Zusammenfassend stellt die Studie eine umfassende und kontextualisierte Konzeption einer Vermittlungsplattform dar, und liefert damit sowohl für den wissenschaftlichen Diskurs im Bereich Gesundheits-IT als auch für Stakeholder aus der Praxis relevante Anhaltspunkte für die Gestaltung und Konzeption zukünftiger Plattformen.
Reliability of COVID-19 data: An evaluation and reflection
Authors: Miller April R. , Charepoo Samin , Yan Erik , Frost Ryan W. , Sturgeon Zachary J. , Gibbon Grace , Balius Patrick N. , Thomas Cedonia S. , Schmitt Melanie A. , Sass Daniel A. , Walters James B. , Flood Tracy L. , Schmitt Thomas A. ,   on behalf of the COVID-19 Data Project
Published: November 2022

Importance
The rapid proliferation of COVID-19 has left governments scrambling, and several data aggregators are now assisting in the reporting of county cases and deaths. The different variables affecting reporting (e.g., time delays in reporting) necessitates a well-documented reliability study examining the data methods and discussion of possible causes of differences between aggregators.

Objective
To statistically evaluate the reliability of COVID-19 data across aggregators using case fatality rate (CFR) estimates and reliability statistics.

Design, setting, and participants
Cases and deaths were collected daily by volunteers via state and local health departments, as primary sources and newspaper reports, as secondary sources. In an effort to begin comparison for reliability statistical analysis, BroadStreet collected data from other COVID-19 aggregator sources, including USAFacts, Johns Hopkins University, New York Times, The COVID Tracking Project.

Main outcomes and measures
COVID-19 cases and death counts at the county and state levels.

Results
Lower levels of inter-rater agreement were observed across aggregators associated with the number of deaths, which manifested itself in state level Bayesian estimates of COVID-19 fatality rates.

Conclusions and relevance
A national, publicly available data set is needed for current and future disease outbreaks and improved reliability in reporting.
The COVID HOME study research protocol: Prospective cohort study of non-hospitalised COVID-19 patients
Authors: Tami Adriana , van der Gun Bernardina T. F. , Wold Karin I. , Vincenti-González María F. , Veloo Alida C. M. , Knoester Marjolein , Harmsma Valerie P. R. , de Boer Gerolf C. , Huckriede Anke L. W. , Pantano Daniele , Gard Lilli , Rodenhuis-Zybert Izabela A. , Upasani Vinit , Smit Jolanda , Dijkstra Akkelies E. , de Haan Jacco J. , van Elst Jip M. , van den Boogaard Jossy , O’ Boyle Shennae , Nacul Luis , Niesters Hubert G. M. , Friedrich Alex W. 
Published: November 2022

Background
Guidelines on COVID-19 management are developed as we learn from this pandemic. However, most research has been done on hospitalised patients and the impact of the disease on non-hospitalised and their role in transmission are not yet well understood. The COVID HOME study conducts research among COVID-19 patients and their family members who were not hospitalised during acute disease, to guide patient care and inform public health guidelines for infection prevention and control in the community and household.

Methods
An ongoing prospective longitudinal observational study of COVID-19 outpatients was established in March 2020 at the beginning of the COVID-19 pandemic in the Netherlands. Laboratory confirmed SARS-CoV-2 infected individuals of all ages that did not merit hospitalisation, and their household (HH) members, were enrolled after written informed consent. Enrolled participants were visited at home within 48 hours after initial diagnosis, and then weekly on days 7, 14 and 21 to obtain clinical data, a blood sample for biochemical parameters/cytokines and serological determination; and a nasopharyngeal/throat swab plus urine, stool and sperm or vaginal secretion (if consenting) to test for SARS-CoV-2 by RT-PCR (viral shedding) and for viral culturing. Weekly nasopharyngeal/throat swabs and stool samples, plus a blood sample on days 0 and 21 were also taken from HH members to determine whether and when they became infected. All participants were invited to continue follow-up at 3-, 6-, 12- and 18-months post-infection to assess long-term sequelae and immunological status.
DNA damage and antioxidant capacity in COPD patients with and without lung cancer
Authors: dos Santos Camila Freitas , Braz Mariana Gobbo , de Arruda Nayara Micarelli , Caram Laura , Nogueira Duelene Ludimila , Tanni Suzana Erico , de Godoy Irma , Ferrari Renata 
Published: November 2022

Background and objectiveChronic obstructive pulmonary disease (COPD) is characterized by chronic inflammation of the lower airways, and COPD patients show two to five times higher risk of lung cancer than smokers with normal lung function. COPD is associated with increased oxidative stress, which may cause DNA damage and lung carcinogenesis. Our aim was to evaluate DNA damage and oxidative stress (lipid peroxidation and antioxidant status) and their relationship in patients with COPD with and without lung cancer.MethodsWe evaluated 18 patients with COPD, 18 with COPD with lung cancer, and 18 controls (former or current smokers). DNA damage was evaluated in peripheral blood lymphocytes using a comet assay; the concentration of malondialdehyde (MDA) and hydrophilic antioxidant performance (HAP) were measured in the plasma.ResultsDNA damage was higher in patients with COPD with cancer than in the controls (p = 0.003). HAP was significantly lower in patients with COPD with cancer than in those without cancer and controls. The presence of lung cancer and COPD showed a positive association with DNA strand breaks and the concentration of MDA.ConclusionCOPD with lung cancer was associated with elevated DNA damage in peripheral lymphocytes, and cancer and COPD showed a positive correlation with DNA damage. The antioxidant capacity showed a negative association with the interaction COPD and cancer and presence of COPD. The mechanisms underlying the increased incidence of lung cancer in COPD are unknown; DNA damage may be involved. Further research may provide insights into their development and treatment.
A mixed-methods study of factors influencing postpartum intrauterine device uptake after family planning counseling among women in Kigali, Rwanda
Authors: Tounkara Mariama S. , Ingabire Rosine , Comeau Dawn L. , Karita Etienne , Allen Susan , Nyombayire Julien , Parker Rachel , Haddad Lisa B. , Da Costa Vanessa , Tichacek Amanda , Mazzei Amelia , Mukamuyango Jeannine , Wall Kristin M. 
Published: November 2022

IntroductionRwanda has high unmet need for family planning (FP), especially in the postpartum period when women are advised to space pregnancies at least two years for improved maternal-child health. Despite interest in the copper intrauterine device (IUD), a highly cost-effective method, access and uptake remain low. This study aimed to determine factors associated with postpartum IUD (PPIUD) uptake after postpartum family planning (PPFP) counseling as well as provider perceptions of facilitators and barriers to clients’ PPIUD uptake.MethodsPostpartum women who received PPFP counseling and were less than 6 weeks postpartum were recruited for a case-control study in Kigali, Rwanda in 2018. We recruited n = 74 women who had accepted and n = 91 women who had declined the PPIUD. Multivariate logistic regression analyses evaluated associations between women’s socio-demographics, FP knowledge and decision-making, and the outcome of PPIUD uptake. Six focus groups (FGs) were conducted with FP providers (n = 24) and community health workers (n = 17) trained to deliver PPFP counseling to assess perceptions of PPFP counseling and facilitators and barriers to PPIUD uptake. FG discussions were recorded, translated, and analyzed for themes.ResultsFactors associated (P<0.1) with PPIUD uptake included citing its non-hormonal nature, effectiveness, and duration of protection against pregnancy as advantages. Exclusive male partner control over FP decisions (relative to women’s control or joint decision-making) was associated with non-use. Overall, limited knowledge about some aspects of the PPIUD persisted among clients even after counseling. Provider FGs highlighted client concerns, inconsistent FP messaging, and lack of male partner involvement as factors influencing non-use.ConclusionsKnowledge of the IUD and its benefits was associated with PPIUD uptake. There is need to refine PPFP counseling messages to address remaining knowledge gaps and concerns. Additionally, male partner involvement in FP counseling and decisions with their partners could be a key strategy to increase both PPIUD and FP uptake in Rwanda.
SPARE-Tau: A flortaucipir machine-learning derived early predictor of cognitive decline
Authors: Toledo Jon B. , Rashid Tanweer , Liu Hangfan , Launer Lenore , Shaw Leslie M. , Heckbert Susan R. , Weiner Michael , Seshadri Sudha , Habes Mohamad ,   for the Alzheimer’s Disease Neuroimaging Initiative
Published: November 2022

BackgroundRecently, tau PET tracers have shown strong associations with clinical outcomes in individuals with cognitive impairment and cognitively unremarkable elderly individuals. flortaucipir PET scans to measure tau deposition in multiple brain areas as the disease progresses. This information needs to be summarized to evaluate disease severity and predict disease progression. We, therefore, sought to develop a machine learning-derived index, SPARE-Tau, which successfully detects pathology in the earliest disease stages and accurately predicts progression compared to a priori-based region of interest approaches (ROI).Methods587 participants of the Alzheimer’s Disease Neuroimaging Initiative (ADNI) cohort had flortaucipir scans, structural MRI scans, and an Aβ biomarker test (CSF or florbetapir PET) performed on the same visit. We derived the SPARE-Tau index in a subset of 367 participants. We evaluated associations with clinical measures for CSF p-tau, SPARE-MRI, and flortaucipir PET indices (SPARE-Tau, meta-temporal, and average Braak ROIs). Bootstrapped multivariate adaptive regression splines linear regression analyzed the association between the biomarkers and baseline ADAS-Cog13 scores. Bootstrapped multivariate linear regression models evaluated associations with clinical diagnosis. Cox-hazards and mixed-effects models investigated clinical progression and longitudinal ADAS-Cog13 changes. The Aβ positive cognitively unremarkable participants, not included in the SPARE-Tau training, served as an independent validation group.ResultsCompared to CSF p-tau, meta-temporal, and averaged Braak tau PET ROIs, SPARE-Tau showed the strongest association with baseline ADAS-cog13 scores and diagnosis. SPARE-Tau also presented the strongest association with clinical progression in cognitively unremarkable participants and longitudinal ADAS-Cog13 changes. Results were confirmed in the Aβ+ cognitively unremarkable hold-out sample participants. CSF p-tau showed the weakest cross-sectional associations and longitudinal prediction.DiscussionFlortaucipir indices showed the strongest clinical association among the studied biomarkers (flortaucipir, florbetapir, structural MRI, and CSF p-tau) and were predictive in the preclinical disease stages. Among the flortaucipir indices, the machine-learning derived SPARE-Tau index was the most sensitive clinical progression biomarker. The combination of different biomarker modalities better predicted cognitive performance.