bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alterations in Circulating Levels of Vitamin D Binding Protein, Total and Bioavailability of Vitamin D in Diabetes Retinopathy Patients
Authors: Maghbooli Zhila , Meimand Sepideh Ebrahimi , Hosseini Ali-asghar Malek , Shirvani Arash 
Published: February 2022

Abstract
AimsThe aims of this study were to investigate the association between circulating levels of vitamin D binding protein (VDBP) and its genotypes and diabetic retinopathy risk.MethodsIn this study, 154 patients with type 2 diabetes mellitus; 62 with diabetic retinopathy (DR) and 92 without DR/and or diabetic nephropathy (DN), were recruited. Circulating levels of 25-hydroxyvitamin D3 and VDBP levels were measured in the patients. The genotype and phenotype of VDBP were evaluated based on two common VDBP variations; rs7041 and rs4588.ResultsSerum levels of VDBP were significantly lower in patients with DR than in patients without DR/and or DN (Ln-VDBP (μg/ml): 6.14±0.92 vs. 6.73±1.45, p = 0.001) even after adjustment for age, sex, body mass index, disease duration, eGFR, HbA1C, and serum levels of 25(OH)D. The distribution of VDBP phenotypes and genotypes in the two studied groups were nearly the same, and the distribution was similar to that of the general population.Conclusions In this study, we found out the association between lower levels of VDBP and risk of DR. However, the precise mechanism linking these two remains unknown. Further and more in-depth research to find out the underlying causes of the relationship is needed.
Large spontaneous HBV DNA fluctuations and potential usefulness of a single point measurement of combined HBV DNA and quantitative HBsAg for the exclusion of HBeAg negative chronic hepatitis B: A prospective Tunisian cohort study
Authors: Chtourou Amel , Gargouri Saba , Elleuch Emna , Fki-Berrajah Lamia , Smaoui Fahmi , Taktak Awatef , Mnif Khouloud , Kassis Mondher , Hammami Adnene , Jemaa Mounir Ben , KARRAY HELA 
Published: February 2022

Abstract
Background/Aims: We aimed to describe spontaneous short-term hepatitis B Virus (HBV) DNA level fluctuations and to assess the usefulness of quantitative HBsAg (qHBsAg) in Tunisian patients with HBeAg-negative chronic HBV infection. Patients and methods: We included 174 treatment-naïve patients with chronic HBeAg-negative HBV. A one-year prospective follow-up was carried out with serial determinations of HBV DNA, alanine aminotransferase levels and qHBsAg. Patients were classified into three groups: inactive carriers (G1), patients with HBeAg negative chronic hepatitis B (CHB) (G2) and patients with “indeterminate state” (G3). For this latter group, a liver biopsy was indicated. Results: Only genotype D was detected. During the follow-up, 21.6% and 19.5% of patients with low initial (<2000 IU/mL) and intermediate viral load (2000-20000 IU/mL), experienced a subsequent increase in their HBV DNA levels above 2000 and 20000 IU/mL, respectively. Significant variations of HBV DNA levels (≥0.5 log 10 IU/mL) were observed in 61.1% of patients at 6 months-interval. Among the 174 patients, 89 (51.1%) belonged to G1, 33 (19%) to G2 and 52 (29.9%) to G3. Fourteen patients have undergone liver biopsy, among whom 7 (50%) showed moderate to severe liver disease. Combination of HBV DNA <2000 IU/mL and qHBsAg <832 IU/mL excluded CHB in 98.4% of cases. Conclusions: This study highlights the large short-term HBV DNA fluctuations in Tunisian patients with HBeAg negative chronic HBV of genotype D. HBV DNA < 2000 IU/mL along with qHBsAg < 832 IU/mL excluded CHB in 98.4% of cases. Significant proportion of patients with “indeterminate state” within genotype D would have HBeAg negative CHB.
RSAP-Net:Joint Optic Disc And Cup Segmentation With A Residual Spatial Attention Path Module And MSRCR-PT Pre-Processing Algorithm
Authors: Jiang Yun , Ma Zeqi , Wu Chao , Zhang Zequn , Yan Wei 
Published: February 2022

Abstract
Background: Glaucoma can cause irreversible blindness to people’s eyesight. Since there are no symptoms inits early stage, it is particularly important to accurately segment the optic disc (OD) and optic cup (OC) fromfundus medical images for the screening and prevention of glaucoma. In recent years, the mainstream methodof OD and OC segmentation is convolution neural network (CNN). However, most existing CNN methodssegment OD and OC separately and ignore the a priori information that OC is always contained inside the ODregion, which makes the segmentation accuracy of most methods not high enough. Methods: This paper proposes a new encoder-decoder segmentation structure, called RSAP-Net, for jointsegmentation of OD and OC. We first designed an efficient U-shaped segmentation network as the backbone.Considering the spatial overlap relationship between OD and OC, a new Residual spatial attention path isproposed to connect the encoder-decoder to retain more characteristic information. In order to further improvethe segmentation performance, a pre-processing method called MSRCR-PT has been devised. It incorporates amulti-scale Retinex colour recovery algorithm and a polar coordinate transformation, which can help RSAP-Netto produce more refined boundaries of the optic disc and the optic cup. Results: The experimental results show that our method achieves excellent segmentation performance on theDrishti-GS1 standard dataset. In the OD and OC segmentation effects, the F1 scores are 0.9752 and 0.9012,respectively. The BLE are 6.33 pixels and 11.97 pixels, respectively. Conclusions: This paper presents a new framework for the joint segmentation of optic discs and optic cups,called RSAP-Net. The framework mainly consists of a U-shaped segmentation skeleton and a residual spaceattention path module. The design of a pre-processing method called MSRCR-PT for the OD/OCsegmentation task can improve segmentation performance. The method was evaluated on the publiclyavailable Drishti-GS standard dataset and proved to be effective.
The role of αA-crystallin and lnc-TMPRSS15 when human lens epithelial cells exposed to dexamethosone
Authors: wang lin , wang yuqing , zhao wencheng , liu ping 
Published: February 2022

Abstract
Objective: To identify the protective and anti-apoptotic role of alpha-crystallin related lncRNAs.Methods: We established stable overexpression of αA- and αB-crystallin in human lens epithelial cells (HLECs). All cells were treated with 0.1 μmol, 1 μmol, 10 μmol and 100 μmol Dex for 12 h, 24 h, 36 h, and 48 h then we detected nine related apoptotic proteins and closely related lncRNAs . Results: Based on western blot results, we determined the most significant exposure factors and cell types and performed long non-coding RNA (lncRNA) and mRNA chip scanning using microchip assay. The results of microchip assay were also verified by real-time PCR. We found that αA- and αB-crystallin had a close relationship with the expression of three anti-apoptotic proteins heat shock protein 60 (HSP60), survivin and P53. However, no significant changes in the expression of cytochrome C, Samc, P65, and Fas were found in HLECs (αA-crystallin overexpressed) and in HLECs (αB-crystallin overexpressed). Following lncRNA and mRNA chip scanning and depth analysis, we found that overexpressed αA-crystallin could increase the expression of lnc-TMPRSS15, TMPRSS15 and related CDH12 and could decrease the expression of HSD11B1, lnc-RNF13-2 and lnc-CDH2-1 which may result in the significantly high expression of anti-apoptotic proteins such as HSP60, survivin and P53. Conclusion: Our results showed that αA-crystallin promoted the expression of some anti-apoptotic proteins. Lnc-TMPRSS15 played a very important role which provides a theoretical basis for the mechanism of glucocorticoid-induced cataract.
Study of the flavonoids of Anemone obtusiloba in the alpine meadow and the adaptation mechanism to the environment based on metabolic pathways
Authors: Wanling Lv , Zuojun Liu 
Published: February 2022

Abstract
In this study, the main pigment composition of Anemone obtusiloba was analysed by UV-vis spectrophotometry, HPLC, and LC-MS. The results showed that there were two kinds of pigmentsin A. obtusiloba, carotenoids, and flavonoids The absorbance spectra regularly changed with the depth of flower colour. Based on HPLC and LC-MS analyses, seven kinds of flavonoids were inferred, including luteolin-3-7-O-glucoside, quercetin-3-O-rutoside, quercetin-3-O-galactoside, quercetin-3-O-glucoside, quercetin-3-O-rhamnoside, kaempferol-3-O-sophoroside, and myricetin-3-O-rhamnose, and the derivative of quercetin was identified as the main component in A. obtusiloba. Besides, according to the analysis of the flavonoid metabolism pathway, it could be determined that flavonoid 3'-hydroxylase (F3′H) was the key enzyme to increase the content of quercetin. This paper speculates that A. obtusiloba increases the content of quercetin by regulating flavonoid F3′H, so that it can deepen the flower colour to adapt to the environment.
Non-invasive ventilation for preoxygenation before general anesthesia: a systematic review and meta-analysis of randomized controlled trials.
Authors: Chiang Tsai-Lien , Tam Ka-Wai , Chen Jui-Tai , Wong Chung-Shun , Yeh Chun-Ting , Huang Ting-Yun , Ong Jiann-Ruey 
Published: February 2022

Abstract
Background and Objectives:

Preoxygenation is crucial for providing sufficient oxygen reservoir to a patient before intubation and enables the extension of the period between breathing termination and critical desaturation (safe apnoea time). Conventionally, face mask ventilation is used for preoxygenation. Non-invasive ventilation is a new preoxygenation method. The study objective was to compare the outcomes of non-invasive ventilation and face mask ventilation for preoxygenation.
Method:

PubMed, Embase, Cochrane Library, and the ClinicalTrials.gov registry were searched for eligible studies published from database inception to September 2021. Individual effect sizes were standardized, and a meta-analysis was conducted using random effects models to calculate the pooled effect size. Inclusion criteria were randomised controlled trials of comparing the outcomes of non-invasive ventilation or face mask ventilation for preoxygenation in patients scheduled for surgeries. The primary outcome was safe apnea time, and the secondary outcomes were post-operative complications, number of patients who achieved the expired O2 fraction (FeO2) after 3 minutes of preoxygenation, minimal SpO2 during tracheal intubation, partial pressure of oxygen in the arterial blood (PaO2) and partial pressure of carbon dioxide (PaCO2) after preoxygenation, and PaO2 and PaCO2 after tracheal intubation.
Results:

13 trials were eligible for inclusion in this study. Significant differences were observed in safe apnoea time, number of patients who achieved FeO2 90% after preoxygenation for 3 minutes, and PaO2 and PaCO2 after preoxygenation and tracheal intubation. Only in the non-obese subgroup, no significant difference was observed in safe apnoea time (mean difference: 125.38, 95% confidence interval: −12.26 to 263.03).
Conclusion:

Non-invasive ventilation appeared to be more effective than conventional methods for preoxygenation. We recommend non-invasive ventilation based on our results.
Anterior Synechiae After Penetrating Keratoplasty In Infants And Children With Peters’ Anomaly
Authors: Yang Yujing , Xiang Jun , Xu Jianjiang 
Published: February 2022

Abstract
Purpose: To evaluate anterior synechiae (AS) after penetrating keratoplasty (PK) in patients with Peters’ anomaly using anterior segment optical coherence tomography (OCT). Methods: A retrospective cross-sectional study was performed. The medical records of patients diagnosed with Peters anomaly who underwent PK between 2013 and 2018 were reviewed. In addition to basic ophthalmic examinations, images of anterior segment structures were obtained via spectral-domain OCT at baseline and during the postoperative follow-up period. The profiles of postoperative AS and multiple potential risk factors were analyzed.Results: Seventy-one eyes of 58 patients, aged 5 to 23 months, were included. Various extent of postoperative AS were observed in 59 eyes (83.1%). OCT findings revealed graft-host junction synechiae, peripheral anterior synechiae and a combination of both. Disease severity and malapposition of the internal graft-host junction (GHJ) were significantly associated with the formation of postoperative AS. Multivariate regression analysis found that preexisting iridocorneal adhesion (ICA) (odds ratio [OR]=16.639, 95% confidence interval [CI] 1.494-185.294, p=0.022) was positively correlated with postoperative AS, whereas anterior chamber depth (OR=0.009, 95% CI 0.000-0.360, p=0.012), and graft size (OR=0.016, 95% CI 0.000-0.529, p=0.020) were negatively correlated with postoperative AS. In addition, quadrants of preexisting ICA and the width of the host corneal bed were identified as risk factors for increased postoperative AS.Conclusions: Postoperative AS is a relatively common occurrence in Peters’ anomaly patients following PK and is found to be associated with preexisting ICA, a shallow anterior chamber, small graft size, GHJ malappositions and grafts closer to the corneal limbus. These data indicate the need for careful consideration when performing PK on these patients.
Fitting the Causal Model of Marital Relationship Distress Based on the Stigma of Infertility: The Mediating Role of Relational Aggression in Infertile Women
Authors: Mikaeili Niloofar , Salvat Habibeh , Ebadi Matine 
Published: February 2022

Abstract
The aim of this study was to fit the causal model of marital relationship distress based on infertility stigma mediated by relational aggression in infertile women. The research method was descriptive and structural equations. The statistical population of the study consisted of all infertile women who referred to infertility centers and obstetrics and gynecology medical centers in Tehran in the summer of 1400 and due to lack of full access to them 300 people were selected by simple random sampling method and participated in the research through online questionnaire. Data were collected using the Marital Self-Reporting Questionnaire Thakson-Wisman et al. (2009), the Fu et al. (2014) Infertility Stigma, and the Nelson- Carroll (2006) Hidden relational Aggression Questionnaire. Data analysis was performed using structural equation modeling. The results showed that the causal model of the relationship between infertility stigma, relational aggression and marital relationship distress in infertile women was confirmed based on various fit indices. Infertility stigma and associated aggression have a direct effect on the marital turmoil of infertile women; Also, the stigma of infertility through relational aggression has an indirect effect on the marital turmoil of infertile women (P <0.05). It can be effective in reducing the chaos of the marital relationship.
The Use of Co-Design in Developing Physical Activity Interventions for Older Adults: A Scoping Review
Authors: Constantin Natalie , Edward Holly , Ng Hayley , Radisic Anna , Yule Amy , D’Asti Alina , D’Amore Cassandra , Reid Julie C , Beauchamp Marla 
Published: February 2022

Abstract
Background: Promoting physical activity (PA) participation in older adults is important for preserving quality of life and functional independence. Co-design has been shown to increase engagement of end-users in health-related policies and interventions. This scoping review aimed to examine how co-design has been used to develop PA interventions for older adults. Methods: We searched MEDLINE, EMBASE, AMED, and CINAHL. Peer-reviewed primary research studies that met the following criteria were included: had at least one participant aged ≥ 60 years involved in the co-design process and the intervention was delivered to individuals whose mean age was ≥ 60, used co-design methodologies, and any form of PA. After duplicate removal, two or more independent reviewers completed title and abstract and full text screening. Data were extracted from the included studies according to study aims. Results: Of the 29 included studies, 12 different terms were used to describe co-design with variable operational definitions that we consolidated into five proposed components. Fifteen studies engaged users in a consultative way, 13 studies using collaboration, and one study engaged end-users in consumer-control. No studies involved end-users in the dissemination phase. Further, no studies directly measured the effectiveness of the co-design process. Five categories of barriers and facilitators to co-design were identified including frameworks and methodologies, logistics, relationships, participation, and generalizability. Conclusions: There is a large degree of variability in how co-design is used to develop PA interventions for older adults. Our findings can be used by researchers to improve rigor and standardization in this emerging field. OSF registration: osf.io/vsw2m
Changes in the Transcriptomic Profiles of Allohexaploid Wheat in Response to Low Fe Stress
Authors: Wang Yue , Hua Ying-peng , Zhou Ting , Huang Jin-yong , Yue Cai-peng 
Published: February 2022

Abstract
Background: Around the world, especially in aerobic soils, because the solubility of iron (Fe) is very low, plants are often stressed by low Fe tress, and the growth, yield, and quality of crops will be inhibited in the case of severe Fe deficiency. The metabolism of Fe in plants is controlled by a series of complex transport, storage, and regulatory mechanisms to maintain the homeostasis of Fe in cells. Allohexaploid wheat (Triticum aestivum L.) is an important food crop and is more sensitive to low Fe tress. Although some studies have been conducted on the low Fe tress response of different plant species, these mechanisms are still unclear in wheat. Results: According to the results of transmission electron microscope, and paraffin section, under low Fe stress, leaf chlorosis was due to damage to the chloroplast structure, and the effect on root structure was mediated by reducing the rate of cell division in meristems and reducing cell elongation in the elongation zone. ICP-MS showed that low Fe stress significantly limited the absorption of essential elements, including N, Pi, K, Ca, Mg, Fe, Mn, Cu, Zn, and B nutrients. RNA sequencing revealed the transcriptomic changes of wheat under low Fe stress. The results showed that 378 and 2,619 differentially expressed genes (DEGs) were identified in the shoots and roots, respectively. These DEGs were mainly involved in the synthesis of Fe chelating agents, ion transport, photosynthesis, amino acid metabolism, protein synthesis, and other processes. Next, to find the core genes responding to low Fe stress, the gene co-expression network diagram was constructed. The results indicated that TaIRT1b-4A, TaNAS2-6D, TaNAS1a-6A, TaNAS1-6B, and TaNAAT1b-1D might play a key role in dealing with low-Fe stress. Conclusions: These research results might help to fully understand the morphological and molecular responses of plants to low Fe stress, and provide excellent genetic resources for the genetic modification of Fe deficiency crops.
Growth Hormone Supplementation During Ovarian Stimulation Does Not Alter Outcomes In Non-Poor Ovarian Response Patients With Multiple IVF Failures
Authors: Yang Jingwei , Zhang Xiaodong , Jing Guo , Chen Qiaoli , Huang Guoning 
Published: February 2022

Abstract
Background: Growth hormone (GH) is being used in different clinical situations in assisted reproductive technologies (ART), but its role has yet to be determined. The aim of this study was to determine the effect of human growth hormone (GH) supplementation during ovarian stimulation in IVF cycles on non-poor ovarian response (non-POR) patients showing two previous failures of IVF cycles with no clearly identifiable reasons.Method: This is a retrospective cohort study of 139 women who underwent a 3rd IVF cycle with adjuvant GH treatment (n=52), compared retrospectively to patients also received a 3rd IVF treatment but without GH during the same period of time (n=87). Generalized estimating equation (GEE) models were used to evaluate the relation between GH and the cumulative clinical pregnancy rate (CCPR) or the number of available embryos.Results: With multiple cycles the proportion of GnRH-ant was higher in the GH group than GH-free group (GH group vs.GH-free: 1st cycle, 11.54% vs. 8.04%; 2nd cycle, 44.23% vs. 33.33%; 3rd cycle, 69.23% vs. 59.77%; P<0.001). The CCPR of the 3rd cycle was highest in self-paired cycles (GH group(cycle 1st-3rd): 30.77% vs.25.00% vs. 36.54%; GH-free group(cycle 1st-3rd):32.18% vs 28.74% vs 40.23%;P<0.001),the CCPR of the GH-free group was higher than that of the GH group in the 3rd cycle (40.23% vs. 36.54%,P<0.01). The use of fixed-effects GEE models revealed that after adjusting for potential confounders, the application of GH was not significantly associated with an increase in the CCPR or the number of available embryos(OR=1.05[95%CI,0.46–2.47] and 0.98[95%CI,0.66-1.48]; P>0.05).Conclusions: The data obtained herein indicated that GH co-treatment in IVF cycles did not exert any obvious effect on non-POR patients.
An Injectable Photo-cross-linking Silk Hydrogel System Augments Diabetic Wound Healing in Orthopaedic Surgery Through Spatiotemporal Immunomodulation
Authors: Mei Jiawei , Zhou Jun , Kong Lingtong , Dai Yong , Zhang Xianzuo , Song Wenqi , zhu chen 
Published: February 2022

Abstract
Background: The complicated hyperglycaemic and chronic inflammation of diabetic wounds in orthopaedic surgery leads to dysregulated immune cell function and potential infection risk in surgical implants. Immune interventions in diabetic wounds face a possible contradiction between simultaneous establishment of the pro-inflammatory environment in response to potential bacterial invasion and the anti-inflammatory environment required for tissue repair. To study this contradiction and accelerate diabetic-wound healing, we developed a photocurable methacryloxylated silk fibroin hydrogel (Sil-MA) system, co-encapsulated with metformin-loaded mesoporous silica microspheres (MET@MSNs) and silver nanoparticles (Ag NPs). Results: The hydrogel system (M@M–Ag–Sil-MA) enhanced diabetic-wound healing via spatiotemporal immunomodulation. Sil-MA imparts a hydrogel system with rapid in situ Ultra-Violet-photocurable capability and allows preliminary controlled release of Ag NPs, which can inhibit bacterial aggregation and create a stable, sterile microenvironment. The results confirmed the involvement of Met in the immunomodulatory effects following spatiotemporal dual-controlled release via the mesoporous silica and Sil-MA, which resulted in induction of macrophage polarisation toward the anti-inflammatory M2-phenotype. Simultaneously, the M@M–Ag–Sil-MA system inhibited the formation of neutrophil extracellular traps (NETs) and decreased the release of neutrophil elastase, myeloperoxidase, and NETs-induced pro-inflammatory factors. Additionally, the M@M–Ag–Sil-MA system promoted fibroblast migration and endothelial cell angiogenesis in vivo, with verification of enhanced diabetic-wound healing accompanied with the spatiotemporal immunoregulation of macrophages and NETs in a diabetic mouse model.Conclusions: Our findings demonstrated that the M@M–Ag–Sil-MA hydrogel system resolved the immune contradiction in diabetic wounds through spatiotemporal immunomodulation of macrophages and NETs, suggesting its potential as a promising engineered nano-dressing for the treatment of diabetic wounds in orthopaedic surgery.