bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Case of Atypical Rabies with Adams-Stokes Syndrome ： a case report
Authors: Song Yuwei , Wang Miaomiao , Jiang Hongyu , Wang Yujie , Zhu Lin , Liu Ning , Zhu Bin 
Published: December 2022

Abstract
Background
Rabies is an infectious disease of animal origin caused by Rabies lyssavirus, commonly known as the Rabies virus (RABV), and leads to acute encephalitis or meningoencephalitis. However, no single test is sufficient for a precise diagnosis. Moreover, the diagnosis of rabies is also difficult for individuals with atypical symptoms or multizonal failures, which lead to a higher rate of clinical misdiagnosis. Therefore, there is an urgent need to develop a sensitive method to diagnose such patients.
Case presentation:
We report a case of atypical rabies with Adams-Stokes syndrome. A 70-year-old male was admitted to hospital with vomiting for five consecutive days and recurrent loss of consciousness for three days. Electrocardiography monitoring showed frequent sinus arrest and third-degree atrioventricular block, suggesting Adams-Stokes syndrome. Percutaneous external noninvasive pacing therapy was immediately administered. On the third day of admission, the patient tested positive for RABV in bronchoalveolar lavage fluid (BALF) using metagenomic next-generation sequencing (mNGS). The patient then developed psychiatric symptoms such as convulsions and eventually deteriorated to multiple organ failure on the fifth day of admission. The patient’s disease developed rapidly after showing typical symptoms of rabies and the patient died on the same day that he voluntarily discharged.
Conclusion
We demonstrated that mNGS could be used as a complementary tool for diagnosing rabies, especially for individuals with unclear exposure histories or atypical symptoms.
The secondary metabolites of Bacillus subtilis strain Z15 Induce Apoptosis in Hepatocellular Carcinoma Cells
Authors: Aimaier Reyihanguli , Li Haoran , Cao Wenzhi , Cao Xiyuan , Zhang Hui , You Jia , Zhao Jingjing , Zhang Qi , Yin Li , Mei Qinshuang , Zhang Xiaorong , Wang Weiquan , Zhao Huixin , Li Jinyu , Zhao Heping 
Published: December 2022

Abstract
The lipopeptides produced by Bacillus subtilis have anti-cancer potential. We had previously identified a secondary metabolite of B. subtilis strain Z15 (BS-Z15), which has an operon that regulates lipopeptide synthesis, and also demonstrated that the fermentation products of this strain exerted antioxidant and pro-immune effects. The purpose of this study was to investigate in vitro and in vivo the anticancer effects of BS-Z15 secondary metabolites (BS-Z15 SMs) on hepatocellular carcinoma (HCC) cells. BS-Z15 SMs significantly inhibited H22 cell-derived murine xenograft tumor growth without any systemic toxicity. In addition, BS-Z15 SMs decreased the viability of H22 cells and BEL-7404 cells in vitro with respective IC50 values of 33.83µg/mL and 27.26 µg/mL. Consistent with this, BS-Z15 SMs induced apoptosis and G0/G1 phase arrest in the BEL-7404 cells, and the mitochondrial membrane potential was also significantly reduced in a dose-dependent manner. Mechanistically, BS-Z15 SMs upregulated the pro-apoptotic p53, Bax, cytochrome C and cleaved-caspase-3/9 proteins, and downregulated the anti-apoptotic Bcl-2.These findings suggest that the induction of apoptosis in HCC cells by BS-Z15 SMs may be related to the mitochondrial pathway. Thus, the secondary metabolites of B. subtilis strain Z15 are promising to become new anti-cancer drugs for the clinical treatment of liver cancer.
The use of an individual-based FDG-PET volume of interest approach in mild cognitive impairment: a multi-modality longitudinal follow-up study
Authors: Huang Shu-Hua , Hsiao Wen-Chiu , Huang Chi-Wei , Chang Hsin-I , Ma Mi-Chia , Hsu Shih-Wei , Lee Chen-Chang , Chen Hong-Jie , Lin Ching-Heng , Chang Chiung-Chih 
Published: December 2022

Abstract
Background:
Based on a longitudinal cohort design, the aim of this study was to investigate whether individual-based 18F fluorodeoxyglucose positron emission tomography (18F-FDG-PET) regional signals can predict dementia conversion in patients with mild cognitive impairment (MCI).
Methods:
We included 44 MCI converters (MCI-C), 38 non-converters (MCI-NC), 42 patients with amyloid-positive Alzheimer disease, and 40 cognitively normal controls. Data from annual cognitive measurements, 3D T1 magnetic resonance imaging (MRI) scans, and 18F-FDG-PET scans were used for outcome analysis. An individual-based FDG-PET approach was defined using seven volumes of interest (VOIs), Z transformed using a normal FDG-PET template. Hypometabolism was defined as a Z score < -2 of regional standard uptake value ratio. For the longitudinal cognitive test scores, generalized estimating equations were used. A linear mixed effect model was used to compare the time effect of cortical hypometabolism and cortical thickness degeneration.
Results
The clinical follow-up period was 6.6 ± 3.8 years (range 3.1 to 16.0 years). The cognitive decline trends could differentiate MCI-C from MCI-NC after 3 years of follow-up. In MCI at the first 18F-FDG-PET scan, medial temporal lobe (94.7% sensitivity, 80.5% specificity) and posterior cingulate cortex (89.5% sensitivity, 73.1% specificity) hypometabolism predicted conversion with high accuracy. 18F-FDG-PET hypometabolism preceded dementia conversion at an interval of 3.70 ± 1.68 years and was earlier than volumetric changes.
Conclusions
Our analysis support the use of individual-based 18F-FDG-PET analysis to predict MCI conversion. Changes in 18F-FDG-PET occurred 1 to 8 years prior to dementia conversion, and hypometabolism occurred 2 years before MRI findings.
Investigation of AIDS-related high-risk behaviors and self-testing services among students from traditional Chinese medicine university
Authors: Ji Jingmin , Zhang Zhiqin , Yu Kun , Ye Jianya , Xu Lijun , Kong Jing , Shi Xinli 
Published: December 2022

Abstract

ObjectiveTo investigate the awareness of AIDS self-testing services and associated factors, as well as the cognition of AIDS-related high-risk behaviors in students of traditional Chinese medical universities.Methods: 716 sophomores participated in a questionnaire survey concerning their understanding of AIDS-related high-risk behaviors.Results: Although there had been a decline in accuracy, university students continued to have the best knowledge of HIV blood transfer, mother-to-child transmission, and sexual transmission of AIDS. More than half of the students misbelieved that the transmission was caused by an HIV-positive person sneezing or coughing (57.54%) or by a mosquito or other blood-sucking bug bites (68.44%). Ritual kissing with HIV-infected persons (52.93%) and sharing a bathroom or swimming pool with them (53.91%) were thought to be the ways that HIV spread. Male and female students had different cognition of men who have sex with men risk factors (P < 0.05). Monthly expenses (> 2000 CNY) [AOR = 5.34 (2.92–9.76)] had a higher odds of a good level of AIDS voluntary counseling and self-testing services.Conclusion: Sophomore university students need to learn professional AIDS high-risk behavior knowledge. Targeted and timely HIV risk education should be taught in college courses, focusing on AIDS self-testing education among students.
System design of student status pre-warning management based on big data
Authors: gao qianqian , Pan Dan , Xu Yurui , Li Wenjuan , Song Guangyuan 
Published: December 2022

Abstract
Based on the idea of Big Data and technical framework, filtered out the specific condition data displayed in a visual way to pre-warn to specific groups of students after collating and cleaning the collected data of score of students of various majors in various colleges. This paper analyzed the status and significance of school students' performance management and school status early warning. Then, we introduced big data technology, designed Hadoop-based pre-warning system to standardize the management process of intelligent digital teaching service and pre-warning system based on a large number of students' achievements. Finally, the paper puts forward the key technology of big data and the concrete implementation path of system design.
Mapping Part Characteristics to Machining Capabilities using a Matching Algorithm.
Authors: Peck William Jordan , Finke Daniel A. 
Published: December 2022

Abstract
The service composition problem is a major obstacle that cloud manufacturing platforms must overcome in order to match service providers to consumers such that the requirements of the consumer are met by the service provider. Previous research focuses on supplier selection, quality of service, and semantic web service approaches as a solution to the service composition problem, but rely on subjective aggregations of high-level concepts that provide a distorted perception of the service providers capabilities. Our matching algorithm methodology uses detailed information regarding operation, part, and tooling specific data to create a granular and realistic representation of the service provider. The matching algorithm is validated using a real-world part drawn from the literature and manufacturers in central Pennsylvania. Our findings indicate that the matching algorithm scales linearly as the number of inputs for a parameter increases. The research contribution
of our matching algorithm is significant as its use of detailed machine specific information is novel. Additionally, its inclusion in a cloud manufacturing platform will decrease machine idleness, while providing the customer with an on-demand service that will aid downstream production and design.
SRSF2-P95H decreases JAK/STAT signaling in hematopoietic cells and delays myelofibrosis development in mice
Authors: Solary Eric , Willekens Christophe , Laplane Lucie , Dragher Tracy , Benlabiod Camelia , Papadopoulos Nicolas , LAcout Catherine , Rameau Philippe , Catelain Cyril , Alfaro Alexia , Edmond Valerie , Signolle Nicolas , Marchand Valentine , Droin Nathalie , Hoogenboezem Remco , Schneider Rebekka , Penson Alex , Abdel-Wahab Omar , GIRAUDIER Stéphane , Pasquier Florence , Marty Caroline , Plo Isabelle , Villeval Jean Luc , Constantinescu Stefan N. , Porteu Francoise , Vainchenker William 
Published: December 2022

Abstract
Heterozygous mutation targeting proline 95 in Serine/Arginine-rich Splicing Factor 2 (SRSF2), associates with V617F mutation in Janus Activated Kinase 2 (JAK2) in some myeloproliferative neoplasms (MPNs), most commonly primary myelofibrosis. To explore Srsf2P95H interaction with Jak2V617F, we generated Cre-inducible knock-in mice expressing these mutants under control of the stem cell leukemia (Scl) gene promoter. In transplantation experiments, Srsf2P95H unexpectedly delayed myelofibrosis induced by Jak2V617F and decreased TGFβ1 serum level. Srsf2P95H reduced the competitiveness of transplanted Jak2V617F hematopoietic stem cells while preventing their exhaustion. RNA sequencing of sorted megakaryocytes identified an increased number of splicing events when the two mutations were combined. Focusing on JAK/STAT pathway, Jak2 exon 14 skipping was promoted by Srsf2P95H, an event detected in patients with JAK2V617F and SRSF2P95 co-mutation. The skipping event generates a truncated inactive JAK2 protein. Accordingly, Srsf2P95H delays myelofibrosis induced by the thrombopoietin receptor agonist Romiplostim in Jak2 wildtype animals. These results unveil JAK2 exon 14 skipping promotion as a strategy to reduce JAK/STAT signaling in pathological conditions.
Swyer-James-MacLeod Syndrome in a Patient With Eisenmenger Syndrome
Authors: Gürses Dolunay , Oğuz Merve , Yüksel Doğangün , Ufuk Furkan , Yılmaz Münevver 
Published: December 2022

Abstract
Swyer-James-MacLeod syndrome is a condition characterized radiologically by hyperlucency in a single lung lobe associated with reduced vascularity, alveolar hyperdistention, and air trapping in the absence of bronchial airway obstruction. Ventricular septal defect is the most common congenital heart defect in childhood and causes irreversible pulmonary hypertension and Eisenmenger syndrome if not treated in a timely manner. In this case report, we present a 25-year-old patient with Swyer-James-MacLeod syndrome and Eisenmenger syndrome. Including Swyer-James-MacLeod syndrome in the differential diagnosis of patients with atypically distributed pulmonary emphysema and unilateral hyperlucency is important for early diagnosis and timely treatment.
Fuzzy Radon Hazard Index Assessment for Stochastic Environmental Health Risk Evaluation of Urban Scale Building
Authors: Sarkheil Hamid , Shirkhani Dariush , Azimi Yousef , Talebi Ahmad , Rahbari Shahrokh 
Published: December 2022

Abstract
Radon gas emission is an emerging phenomenon that modern lifestyles have become a potential danger to humans. So, stochastic evaluation of the amount of this hazardous gas in urban scale and residential buildings can be critical in identifying environmental health risks. A novel fuzzy Radon Hazard Index is proposed in this research to assess statistical radon environmental health risks. The output index FRHI ranges from 0 (No Hazard) to 100 (The highest degree of hazard). The approach can serve as a circumstantially integrated standard for stochastic radon risk assessment and management because innovation fuzzifies this field's most important stochastic standards. In this study, first, Radon was measured in the urban scale building, and then, Arc GIS software prepared natural Radon emission zoning maps at urban and geological scales. A residential building unit in one of the critical areas has been selected. Some corrective actions have been adopted to reduce Radon in urban building units. The FRHI Assessment for stochastic environmental health risk evaluation shows that the initial fuzzy level for the mean value of FRHI is Hazardous (for FRHI value equal to 60.1) determined by Red color. This is while the Maximum FRHI level for 48 hours after installation is Rather Hazardous (for FRHI value equal to 44.8) determined with orange color, and the maximum statistical environmental health risk after steady-state installation would fall into an improved category. So identifying critical areas can provide exceptional control at the urban scale building that reduces the risks of natural Radon.
A dynamic epibiont community associated with the bone eating worm Osedax
Authors: Goffredi Shana , Panossian Balig , Brzechffa Camille , Field Naomi , King Chad , Moggioli Giacomo , Rouse Greg W. , Martín-Durán José M. , Henry Lee 
Published: December 2022

Abstract

BackgroundOsedax,the deep-sea annelid found at sunken whalefalls, is known to host bacterial endosymbionts intracellularly in specialized roots, that help it feed exclusively on vertebrate bones. Past studies, however, have also made mention of external bacteria on their trunks. Here, we present an examination of the bacterial communities associated with the external surfaces of seven species ofOsedaxworms. Using molecular, metagenomic, and microscopy analyses we reveal a dynamic community of Campylobacterales epibionts associated withOsedaxthat are unique from close relatives and metabolically suited to different successional stages of whale decomposition.ResultsDuring this 14-year study, we reveal a dynamic, yet persistent, succession of Campylobacterales epibionts integrated into the epidermis ofOsedax, that change over time as the whale carcass degrades on the sea floor. The epibionts associated with seven species ofOsedax, which comprise 67% of the bacterial community on the trunk, are initially dominated by the genusArcobacter(at early time points < 24 months), theSulfurospirillumat intermediate stages (~ 50 months), and theSulfurimonasat later stages (>140 months) of whale carcass decomposition. Metagenome analysis of the epibiont metabolic capabilities suggests a transition from heterotrophy to autotrophy along the successional gradient, and differences in their capacity to metabolize oxygen, carbon, nitrogen, and sulfur. Compared to free living relatives, theOsedaxepibionts were highly enriched in transposable elements, implicating genetic exchange on the host surface, and contained numerous secretions systems with enriched effector proteins having eukaryotic-like domains.ConclusionsDiverse bacteria form non-transient associations with the external surfaces of eukaryotes and can contribute to the health and physiology of their hosts. The recurrence of three Campylobacterales associated with diverseOsedaxspecies collected from multiple deep-sea locations suggests they are specific epibionts that share a long-evolutionary history with these enigmatic, yet widely distributed deep-sea worms. All three epibionts have an affinity for organic-rich and sulfide-rich habitats, however a successional shift in their composition reveals that they are a dynamic community that changes over time. These results provide evidence of a persistent yet dynamic relationship betweenOsedaxand specific Campylobacterales epibionts that possess unique genomic features.
Post-harvest protective applications of rhamnolipid producing phyllospheric Pseudomonas sp. MP 11 isolated from Musa acuminata
Authors: George Maria Liz , Theresa Mary , Geevarghese Amala , Anilkumar Athira , K Radhakrishnan E 
Published: December 2022

Abstract
Microorganisms residing within the diverse parts of plants play a significant role in the plant growth and defence response. Hence, the current study emphasized the isolation and screening of plant-beneficial traits of isolates obtained from leaves of Musa acuminata cultivated in Kottayam, Kerala. Among the many isolates obtained during screening, MP 11 was found to have promising activity against the tested phytopathogens viz. Fusarium rolfsii, Pythium aphanidermatum, Phytophthora infestans, Sclerotium rolfsii. This isolate was further identified as Pseudomonas sp. by the 16S rRNA-based sequence analysis. Additionally, the isolate was analysed for various plant growth promoting traits, whereby it showed its capability to solubilise zinc and phosphate, IAA, HCN and ACC deaminase production. MP 11 also showed potential to provide post-harvest protection to the rice seeds from P. aphanidermatum and S. rolfsii. Based on all these properties, the isolated PGPB could be developed into a formulation which would induce multiple beneficial impacts in the production of rice.
Can habitat preferences of ground-dwelling insects be a good indicator for terrestrial ecosystem recovery after an oil-spill?
Authors: Segev Nitzan , Groner Elli , Bouskila Amos , Berger-Tal Oded 
Published: December 2022

Abstract
Although oil spills are a major source of global pollution, little is known about the effects of oil spills on animals in terrestrial ecosystems. We investigated two oil spills that occurred in 1975 and 2014 in a nature reserve within a hyper-arid desert, aiming at determining the long- and short-term effects of oil spills on the habitat selection behavior of desert insects, as a possible behavioral indicator for restoration success. We performed habitat selection experiments under lab conditions, giving ground-dwelling Mesostena angustata beetles and Myrmeleon hyalinus antlion larvae choice trials between various combinations of clean and oil-contaminated soils from the new and old oil spills sites. When given a choice between clean and 2014 contaminated soil, beetles and antlions selected the clean soil. Moreover, antlions in contaminated soil from 2014 reduced their movements and did not dig pit-traps. Surprisingly, both beetles and antlions selected the 1975 contaminated soil over other soil types, and antlions dug many pit-traps in the 1975 contaminated soil.
Implications for insect conservation: The antlions' behavior reflects the status of its population in the field and can be used as an indicator for habitat restoration. In contrast, the beetle's behavior may not be a reliable behavioral indicator. Beetles were scarce in 1975 contaminated areas in the field. The disparity between individual behavior of beetles in the lab and field population distribution raised the question: Can old oil spills become an ecological trap for these species? This remains an open question for further research.
SARS-CoV2 mutations and impact on mortality: observational study in a sub- Saharan Africa hospital
Authors: Makulo Jean-Robert , Mbala Placide , Nlandu Yannick , Kabwe Benjanmin , Wumba Roger , Aziza Adrienne Amuri , Mandina Madone Ndona , Mangala Donatien , Bepouka Ben Izizag , Odio Jerome Ossam , Longokolo Murielle , Mukenge Eric K , Kamwiziku Guyguy , Kingand Eddy Lusamaki , Bashengezi Constantin , Kabanda Gilbert , Longo-Mbenza Benjamin 
Published: December 2022

Abstract
Background
One year after the COVID-19 pandemic, the focus of attention has shifted to the emergence and spread of SARS-CoV-2 variants of concern (VOCs). The aim of the study was to assess the frequency of VOCs in patients followed for COVID-19 at Kinshasa university hospital (KUH) during the 3rd and 4th waves of the pandemic in Kinshasa. Hospital mortality was compared to that of the first two waves.
Method
The present study was conducted from June 2021 to January 2022 and included all patients in whom the diagnosis of SARS-CoV-2 infection was confirmed by the polymerase chain reaction (PCR). The laboratory team sequenced a subset of all SARS-CoV-2 positive samples with high viral loads define as Ct < 25 to ensure the chances to generate complete genome sequence. RNA extraction was performed using the Viral RNA Mini Kit (Qiagen). Depending on the platform, we used the iVar bioinformatics or artic environments to generate consensus genomes from the raw sequencing output in FASTQ format.
Results
During the study period, the original strain of the virus was no longer circulating. The Delta VOC was predominant from June (92%) until November 2021 (3rd wave). The Omicron VOC, which appeared in December 2021, became largely predominant one month later (96%) corresponding the 4th wave. In-hospital mortality associated with COVID-19 fell during the 2nd wave (7% vs 21% 1st wave), had risen during the 3rd (16%) wave before falling again during the 4th wave (7%) (p < 0.001).
Conclusion
The Delta (during the 3rd wave) and Omicron VOCs (during the 4th wave) were very predominant among patients followed for Covid-19 in our hospital. Contrary to data in the general population, hospital mortality associated with severe and critical forms of COVID-19 had increased during the 3rd wave of the pandemic in Kinshasa.