bluuebunny/crossref_metadata_2025_split huggingface dataset:
The global dynamic of DNA methylation in response to heat stress revealed epigenetic mechanism of heat acclimation in Saccharina japonica
Authors: Liu Fuli , Zhang Pengyan , Liang Zhourui , Yuan Yanmin , Liu Yi , Wu Yukun 
Published: December 2022

Saccharina japonica is an ecologically and economically important kelp in cold‐temperate regions. When it is cultivated on a large scale in the temperate and even subtropical zones, heat stress is a frequent abiotic stress. This study is the first attempt to reveal the regulatory mechanism of the response to heat stress from the perspective of DNA methylation in S. japonica. We firstly obtained the characteristics of variation in the methylome under heat stress, and observed that heat stress caused a slight increase in the overall methylation level and methylation rate, especially in the non‐coding regions of the genome. Secondly, we noted that methylation was probably one of factors affecting the expression of genes, and that methylation within the gene body was positively correlated with the gene expression (rho = 0.0784). Moreover, it was found that among the differentially expressed genes regulated by methylation, many genes were related to heat stress response, such as HSP gene family, genes of antioxidant enzymes, genes related to proteasome‐ubiquitination pathway, and plant cell signaling pathways. This study demonstrated that DNA methylation is involved in regulating the response to heat stress, laying a foundation for studying the acclimation and adaptation of S. japonica to heat stress from an epigenetic perspective.
Questioning Consilience and Autonomy in Self‐Determination Theory: A Critique and Hermeneutic‐Phenomenological Alternative
Authors: Gantt Edwin E. , Yanchar Stephen C. , Parker Jared C. 
Published: December 2022

AbstractThis paper offers a theoretical analysis of Self‐Determination Theory (SDT) and its claims regarding human autonomy. Self‐determination theorists have advanced a form of self‐regulated, engaged behavior (i.e., autonomy), founded upon on a consilient account of human motivation that assumes multiple, hierarchical levels of organization and causation (e.g., biological, psychological, and social). Autonomy, from this perspective, is taken to emerge from underlying biological mechanisms, but also able to exert its own causal effects in the world as a unique psychological phenomenon. We contend that in theorizing this way, self‐determination theorists have invoked a mixed discourse of mechanism and autonomy that leaves important questions unanswered, perhaps most importantly those concerning how autonomy as a kind of volition can fit coherently in the mechanistic account of world that they advocate. We then offer an alternative perspective based upon the work of various hermeneutic‐phenomenological thinkers in philosophy and psychology. This alternative perspective conceptualizes human phenomena such as autonomy and motivation in agentic terms, emphasizing meaningful participation in possibility‐laden contexts of everyday practices.
Lower frequency of MDSCs was significantly related to functional cure in CHB patients treated with peginterferon
Authors: Zhang Yao , Han Jiajia , Zhang Xueyun , Li Fahong , Guo Yifei , He Jingjing , Mao Richeng , Zhu Haoxiang , Yu Jie , Huang Yuxian , Yang Feifei , Zhang Jiming 
Published: December 2022

AbstractBackground and AimsMyeloid‐derived suppressor cells (MDSCs) and CD4+ regulatory T cells (Tregs) expand during chronic hepatitis B virus (HBV) infection and inhibit antiviral immunity. However, the relationship between antiviral effect and the frequencies of those immune suppressive cells after pegylated interferon α‐2a (PegIFNα‐2a) therapy is not clearly understood. This study aimed to investigate the contribution of monocytic MDSCs (mMDSCs) and CD4+ Tregs to functional cure (HBsAg seroclearance) after PegIFNα‐2a therapy and evaluate the effect of PegIFNα‐2a therapy on these cells.MethodsFlow cytometry analysis was performed along with longitudinal immune monitoring of 97 hepatitis B e antigen (HBeAg) negative chronic hepatitis B (CHB) patients receiving PegIFNα‐2a weekly for 48 weeks.ResultsThe frequencies of mMDSCs and CD4+ Tregs increased in all HBV patients, and they were higher in the HBsAg persistence group than in the HBsAg seroclearance group. A significant decline in the frequency of mMDSCs was found in patients who realized functional cure after PegIFNα‐2a treatment. In contrast, the frequency of CD4+ Tregs in both the HBsAg seroclearance and persistence groups significantly increased. Multivariate analyses indicated that the baseline serum HBsAg levels (p < .001) and mMDSCs frequency (p = .027) were independently associated with the HBsAg clearance, and the combined marker (HBsAg plus mMDSCs) displayed the highest specificity (93.1%) than any other markers in predicting HBsAg seroclearance.ConclusionsThese results suggest that a poor response to PegIFNα‐2a treatment in CHB patients may be related to the frequencies of immune suppressive cells, while the therapeutic targeting of these cells might be effective in boosting anti‐HBV immunity.
Ar‐Ar and U‐Pb ages of Chelyabinsk and a re‐evaluation of its impact chronology
Authors: Beard Sky P. , Swindle Timothy D. , Lapen Thomas J. , Kring David A. 
Published: December 2022

AbstractThe LL5 chondrite Chelyabinsk has had numerous isotopic studies since its fall in 2013. These data have been used to suggest ~8 impact events recorded from multiple isotopic systems (e.g., Ar‐Ar, U–Pb, Sm‐Nd, Rb‐Sr, among others). We report details of Ar‐Ar and U‐Pb results and re‐evaluate the geochronology of Chelyabinsk. Argon has the youngest Ar‐Ar age recorded in meteorites, 25 ± 11 Ma, and an older resetting event at ~2550 Ma. The U‐Pb analysis has an upper concordia age of 4456 ± 23 Ma and a lower concordia age of 184 ± 200 Ma. The lower concordia intercept represents a later thermal event (e.g., an impact), the most recent time that lead loss occurred, and could represent resetting by the youngest event recorded by Ar‐Ar. Combining our data with literature results, we find strong evidence of at least four impact events (~4450, 2550, 1700, 25 Ma), with some evidence for two additional impacts (~3700, 1000 Ma).
Learner engagement and teaching effectiveness in livestreamed versus in‐person CME
Authors: Stephenson Christopher R. , Yudkowsky Rachel , Wittich Christopher M. , Cook David A. 
Published: December 2022

AbstractIntroductionEngaging learners in continuing medical education (CME) is challenging. Recently, CME courses have transitioned to livestreamed CME, with learners viewing live, in‐person courses online. The authors aimed to (1) compare learner engagement and teaching effectiveness in livestreamed with in‐person CME and (2) determine how livestream engagement and teaching effectiveness is associated with (A) interactivity metrics, (B) presentation characteristics and (C) medical knowledge.MethodsA 3‐year, non‐randomised study of in‐person and livestream CME was performed. The course was in‐person for 2018 but transitioned to livestream for 2020 and 2021. Learners completed the Learner Engagement Inventory and Teaching Effectiveness Instrument after each presentation. Both instruments were supported by content, internal structure and relations to other variables' validity evidence. Interactivity metrics included learner use of audience response, questions asked by learners and presentation views. Presentation characteristics included presentations using audience response, using pre/post‐test format, time of day and words per slide. Medical knowledge was assessed by audience response. A repeated measures analysis of variance (anova) was used for comparisons and a mixed model approach for correlations.ResultsA total of 159 learners (response rate 27%) completed questionnaires. Engagement did not significantly differ between in‐person or livestream CME. (4.56 versus 4.53, p = 0.64, maximum 5 = highly engaged). However, teacher effectiveness scores were higher for in‐person compared with livestream (4.77 versus 4.71 p = 0.01, maximum 5 = highly effective). For livestreamed courses, learner engagement was associated with presentation characteristics, including presentation using of audience response (yes = 4.57, no = 4.45, p < .0001), use of a pre/post‐test (yes = 4.62, no = 4.54, p < .0001) and time of presentation (morning = 4.58, afternoon = 4.53, p = .0002). Significant associations were not seen for interactivity metrics or medical knowledge.DiscussionLivestreaming may be as engaging as in‐person CME. Although teaching effectiveness in livestreaming was lower, this difference was small. CME course planners should consider offering livestream CME while exploring strategies to enhance teaching effectiveness in livestreamed settings.
Estimating intraseasonal intrinsic water‐use efficiency from high‐resolution tree‐ring δ13C data in boreal Scots pine forests
Authors: Tang Yu , Sahlstedt Elina , Young Giles , Schiestl‐Aalto Pauliina , Saurer Matthias , Kolari Pasi , Jyske Tuula , Bäck Jaana , Rinne‐Garmston Katja T. 
Published: December 2022

Summary
Intrinsic water‐use efficiency (iWUE), a key index for carbon and water balance, has been widely estimated from tree‐ring δ13C at annual resolution, but rarely at high‐resolution intraseasonal scale.
We estimated high‐resolution iWUE from laser‐ablation δ13C analysis of tree‐rings (iWUEiso) and compared it with iWUE derived from gas exchange (iWUEgas) and eddy covariance (iWUEEC) data for two Pinus sylvestris forests from 2002 to 2019.
By carefully timing iWUEiso via modeled tree‐ring growth, iWUEiso aligned well with iWUEgas and iWUEEC at intraseasonal scale. However, year‐to‐year patterns of iWUEgas, iWUEiso, and iWUEEC were different, possibly due to distinct environmental drivers on iWUE across leaf, tree, and ecosystem scales. We quantified the modification of iWUEiso by postphotosynthetic δ13C enrichment from leaf sucrose to tree rings and by nonexplicit inclusion of mesophyll and photorespiration terms in photosynthetic discrimination model, which resulted in overestimation of iWUEiso by up to 11% and 14%, respectively.
We thus extended the application of tree‐ring δ13C for iWUE estimates to high‐resolution intraseasonal scale. The comparison of iWUEgas, iWUEiso, and iWUEEC provides important insights into physiological acclimation of trees across leaf, tree, and ecosystem scales under climate change and improves the upscaling of ecological models.
Effects of Allogeneic Bone Substitute Configurations on Cell Adhesion Process In Vitro
Authors: Liu Jie , Yang Li , Zhang Hao , Zhang Jing‐yu , Hu Yong‐cheng 
Published: December 2022

ObjectiveTo explore the potential effect of three allogenic bone substitute configurations on the viability, adhesion, and spreading of osteoblasts in vitro.MethodsFreeze‐dried cortical bone were ground and fractions were divided into three groups with different sizes and shapes, defined as bone fiber (0.1 mm × 0.1 mm × 3 mm), bone powder (0.45–0.9 mm), and bone granule group (3–6 mm). MC3T3‐E1 cells were divided and co‐cultured within groups to induce cell adhesion. The configuration of allogenic bone was captured by scanning electron microscopy and confocal laser scanning microscopy, and substrate roughness values were quantified. Cell adhesion rate was assessed using the hemocyte counting method, cell viability was determined by CCK‐8 assay and live/dead staining, and cell morphology was visualized by Phalloidin and DAPI, and the mRNA expression of adhesion‐related gene (vinculin) of different substitutes were determined with quantitative real‐time polymerase chain reaction.ResultsThe roughness values of bone fiber, bone powder, and bone granule group were 1.878 μm (1.578–2.415 μm), 5.066 μm (3.891–6.162 μm), and 0.860 μm (0.801–1.452 μm), respectively (bone powder group compared with bone granule group, H = 18.015, P < 0.001). Similar OD values of all groups in CCK‐8 assay indicated good biocompatibility of these substitutes (bone fiber, 0.201 ± 0.004; bone powder, 0.206 ± 0.008; bone granule group, 0.197 ± 0.006; and the control group, 0.202 ± 0.016, F = 0.7152, P > 0.05). In addition, representative cell adhesion rates at 24 h showed significantly lower cell adhesion rate in bone fiber group (20.3 ± 1.6%) compared to bone powder (29.3 ± 4.4%) and bone granule group (27.3 ± 3.2%) (F = 10.51，P = 0.009 and P = 0.034, respectively), but there was no significant difference between the latter two groups (P > 0.05). Interestingly, the expression of vinculin mRNA steadily decreased in a time‐dependent manner. The vinculin expression reached its peak at 6 h in each group, and the vinculin levels in bone fiber, bone powder, and bone granule group were 2.119 ± 0.052, 3.842 ± 0.108, and 3.585 ± 0.068 times higher than those in the control group, respectively (F = 733.643, all P < 0.001). Meanwhile, there was a significant difference in the expression of target gene between bone powder and bone granule group (P = 0.006).ConclusionAll allogenic bone substitutes presented an excellent cell viability. Moreover, bone powder and bone granule group were more likely to promote cell adhesion and spreading compared to bone fiber group.
Computer Navigation Assisted Restricted Kinematic Alignment Improves Short‐Term Outcomes in Total Knee Arthroplasty: An Ambispective Cohort Study
Authors: Ma Ruichen , Chen Xi , Li Haolin , Wang Yiou , Li Songlin , Li Shanni , Qian Wenwei 
Published: December 2022

ObjectivesMechanical alignment (MA)‐total knee arthroplasty (TKA) has been challenged due to the excessive soft tissue release and the evidence of the clinical outcomes of computer assisted navigation is still limited. The aim of this ambispective cohort study was to: (i) investigate whether computer assisted navigation is capable to achieve restricted kinematic alignment (rKA)‐TKA; and (ii) compare the short‐term outcomes between rKA‐TKA and MA‐TKA.MethodsWe retrospectively included 41 patients diagnosed with osteoarthritis who received MA‐TKA between April 2019 and January 2021 and 43 patients diagnosed with osteoarthritis who received rKA‐TKA were included in the prospective cohort from January 2021 to September 2021. Demographical, peri‐operative, and radiological data were collected and compared. Unpaired two‐sample t‐test for continuous variables and χ2 test for categorical variables were used to compare various measurements in two groups. The patient‐reported outcome measures at baseline, 10 days (T1), and 6 months (T6) after surgery were statistically analyzed by generalized estimating equation (GEE) models.ResultsFourty‐one patients (45 knees) and 43 patients (48 knees) were included in the MA and the rKA group respectively. Three constitutional knee phenotypes (II, I, IV) were the commonest in our population. Navigation improved the surgical accuracy (1.5° vs 3.5°, p < 0.001) and precision (interquartile range 4.0 vs 2.0, p < 0.001) in the rKA group than the MA group. The changes in Knee Injury and Osteoarthritis Outcome Score 12 (KOOS12), EuroQol five‐dimension questionnaire (EQ5D) from baseline to T1 and T6 for patients with on‐target rKA were larger than on‐target MA counterparts (26.053 vs 18.607, P < 0.001(KOOS12, T1), 0.457 vs 0.367 p < 0.001(EQ5D, T1); 51.017 vs 46.896, P = 0.023(KOOS12, T6), 0.606 vs 0.565, P = 0.01(EQ5D, T6)). Patients with on‐target rKA had better Forgotten Joint Score (FJS) at T1 (54.126 vs 40.965, P = 0.002) compared with on‐target MA counterparts.ConclusionsComputer assisted navigation achieved the level of accuracy required by rKA‐TKA. rKA‐TKA offered significantly better short‐term outcomes than MA‐TKA.
A Rare Case of Melanotic Schwannoma Occurred Intraosseous of Sacrum: A Literature Review
Authors: Yan Xiaobo , Wang Keyi , Lin Nong , Huang Xin , Fu YanBiao , Ye Zhaoming 
Published: December 2022

BackgroundMelanotic schwannoma is a rare tumor when it occurs in the sacrum. Though it is mostly classified as benign, the prognosis is unpredictable due to the possibility of recurrence and metastasis. Here, we reported a case of intraosseous of sacrum with good results and reviewed the literature.Case PresentationA 33‐year‐old male patient complained of low back pain and was discovered to have an obstruction at S2. Following the necessary imaging diagnosis, we treated the patient with piecemeal excision in conjunction with extended curettage, and the frozen biopsy revealed that the tumor was melanotic schwannoma. The intraosseous portion of the lesion was curettaged using high‐speed drill to enlarge the edge of curettage, and piecemeal excision for lesion within the sacral canal. After surgery, the patient received total 56Gy radiotherapy and frequent follow‐up. After 15 months follow‐up, there was no evidence of recurrence, and the nerve function was normal.ConclusionMelanotic schwannoma that occurs intraosseous of the sacrum is extremely rare and lacks typical clinical manifestations; however it can be identified through careful pathological and imaging diagnosis. Intralesional extended curettage combined with radiotherapy can achieve a good local control with a satisfactory clinical effect in this rare disease.
Meliaceae genomes provide insights into wood development and limonoids biosynthesis
Authors: Cui Gaofeng , Li Yun , Yi Xin , Wang Jieyu , Lin Peifan , Lu Cui , Zhang Qunjie , Gao Lizhi , Zhong Guohua 
Published: December 2022

SummaryMeliaceae is a useful plant family owing to its high‐quality timber and its many limonoids that have pharmacological and biological activities. Although some genomes of Meliaceae species have been reported, many questions regarding their unique family features, namely wood quality and natural products, have not been answered. In this study, we provide the whole‐genome sequence of Melia azedarach comprising 237.16 Mb with a contig N50 of 8.07 Mb, and an improved genome sequence of Azadirachta indica comprising 223.66 Mb with a contig N50 of 8.91 Mb. Moreover, genome skimming data, transcriptomes and other published genomes were comprehensively analysed to determine the genes and proteins that produce superior wood and valuable limonoids. Phylogenetic analysis of chloroplast genomes, single‐copy gene families and single‐nucleotide polymorphisms revealed that Meliaceae should be classified into two subfamilies: Cedreloideae and Melioideae. Although the Meliaceae species did not undergo additional whole‐genome duplication events, the secondary wall biosynthetic genes of the woody Cedreloideae species, Toona sinensis, expanded significantly compared to those of A. indica and M. azedarach, especially in downstream transcription factors and cellulose/hemicellulose biosynthesis‐related genes. Moreover, expanded special oxidosqualene cyclase catalogues can help diversify Sapindales skeletons, and the clustered genes that regulate terpene chain elongation, cyclization and modification would support their roles in limonoid biosynthesis. The expanded clans of terpene synthase, O‐methyltransferase and cytochrome P450, which are mainly derived from tandem duplication, are responsible for the different limonoid classes among the species. These results are beneficial for further investigations of wood development and limonoid biosynthesis.
Assessment of the soluble proteins HMGB1, CD40L and CD62P during various platelet preparation processes and the storage of platelet concentrates: The BEST collaborative study
Authors: Cognasse Fabrice , Hamzeh Cognasse Hind , Eyraud Marie Ange , Prier Amélie , Arthaud Charles Antoine , Tiberghien Pierre , Begue Stephane , de Korte Dirk , Gouwerok Eric , Greinacher Andreas , Aurich Konstanze , Noorman Femke , Dumont Larry , Kelly Kathleen , Cloutier Marc , Bazin Renée , Cardigan Rebecca , Huish Sian , Smethurst Peter , Devine Dana , Schubert Peter , Johnson Lacey , Marks Denese C. ,   Biomedical Excellence for Safer Transfusion (BEST) Collaborative
Published: December 2022

AbstractBackgroundStructural and biochemical changes in stored platelets are influenced by collection and processing methods. This international study investigates the effects of platelet (PLT) processing and storage conditions on HMGB1, sCD40L, and sCD62P protein levels in platelet concentrate supernatants (PCs).Study Design/MethodsPC supernatants (n = 3748) were collected by each international centre using identical centrifugation methods (n = 9) and tested centrally using the ELISA/Luminex platform. Apheresis versus the buffy coat (BC‐PC) method, plasma storage versus PAS and RT storage versus cold (4°C) were investigated. We focused on PC preparation collecting samples during early (RT: day 1–3; cold: day 1–5) and late (RT: day 4–7; cold: day 7–10) storage time points.ResultsHMGB1, sCD40L, and sCD62P concentrations were similar during early storage periods, regardless of storage solution (BC‐PC plasma and BC‐PC PAS‐E) or temperature. During storage and without PAS, sCD40L and CD62P in BC‐PC supernatants increased significantly (+33% and +41%, respectively) depending on storage temperature (22 vs. 4°C). However, without PAS‐E, levels decreased significantly (−31% and −20%, respectively), depending on storage temperature (22 vs. 4°C). Contrastingly, the processing method appeared to have greater impact on HMGB1 release versus storage duration. These data highlight increases in these parameters during storage and differences between preparation methods and storage temperatures.ConclusionsThe HMGB1 release mechanism/intracellular pathways appear to differ from sCD62P and sCD40L. The extent to which these differences affect patient outcomes, particularly post‐transfusion platelet increment and adverse events, warrants further investigation in clinical trials with various therapeutic indications.
Design-as-a-Service Framework for Enabling Innovations in Small- and Medium-Sized Enterprises
Authors: Agarwal Ankit , Sorathiya Pratik C. , Vaishnav Shubham , Desai K. A. , Mears Laine 
Published: December 2022

Abstract
Modern manufacturing enterprises must be agile to cope with sudden demand changes arising from increased global competition, geopolitical factors, and unforeseen circumstances such as the Covid-19 pandemic. Small- and Medium-Sized Enterprises (SMEs) in the manufacturing sector lack agility due to lower penetration of Information Technology (IT) and Operational Technology (OT), the inability to employ highly skilled human capital, and the absence of a formal innovation ecosystem for new products or solutions. In recent years, Cloud-based Design and Manufacturing (CBDM) has emerged as an enabler for product realization by integrating various service-based models. However, the existing framework does not thoroughly support the innovation ecosystem from concept to product realization by formally addressing economic challenges and human skillset requirements. The present work considers the augmentation of the Design-as-a-Service (DaaS) model into the existing CBDM framework for enabling systematic product innovations. The DaaS model proposes to connect skilled human resources with enterprises interested in transforming an idea into a product or solution through the CBDM framework. The model presents an approach for integrating human resources with various CBDM elements and end-users through a service-based model. The challenges associated with successfully implementing the proposed model are also discussed. It is established that the DaaS has the potential for rapid and economical product discovery and can be readily accessible to SMEs or independent individuals.