bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of microRNA-223 and microRNA-146b in serum and liver tissue of mice infected with Schistosoma mansoni
Authors: El-Taweel Hend A , Issa Yasmine A , Mady Rasha F , Shehata Ghada A , Youssef Eman A , Tolba Mona M 
Published: May 2022

AbstractMicroRNAs (miRNAs) play regulatory roles in several diseases. In schistosomiasis, the main pathological changes are caused by the granulomatous reaction induced by egg deposition. We aimed to study the changes in host miRNA-223 and miRNA-146b expression in relation to egg deposition and development of hepatic pathology in murine schistosomiasis mansoni. Blood and liver tissue samples were collected from non-infected mice (group I), S. mansoni–infected mice at the 4th, 8th, and 12th weeks post-infection (p.i.) (groups II–IV), and 4 weeks after praziquantel treatment (group V). The collected samples were processed for RNA extraction, reverse transcription, and real-time PCR analysis of miRNA-223 and miRNA-146b. miRNAs’ relative expression was estimated by the ΔΔCt method. Liver tissue samples were examined for egg count estimation and histopathological evaluation. Results revealed that miRNA-223 was significantly downregulated in liver tissues 8 and 12 weeks p.i., whereas miRNA-146b expression increased gradually with the progression of infection with a significantly higher level at week 12 p.i. compared to week 4 p.i. Serum expression levels nearly followed the same pattern as the tissue levels. The dysregulated expression of miRNAs correlated with liver egg counts and was more obvious with the demonstration of chronic granulomas, fibrous transformation, and distorted hepatic architecture 12 weeks p.i. Restoration of normal expression levels was observed 4 weeks after treatment. Collectively, these findings provide new insights for in-depth understanding of host-parasite interaction in schistosomiasis and pave a new way for monitoring the progress of hepatic pathology before and after treatment.
Effect of urbanization and its environmental stressors on the intraspecific variation of flight functional traits in two bumblebee species
Authors: Tommasi Nicola , Pioltelli Emiliano , Biella Paolo , Labra Massimo , Casiraghi Maurizio , Galimberti Andrea 
Published: May 2022

AbstractThe way urbanization shapes the intraspecific variation of pollinator functional traits is little understood. However, this topic is relevant for investigating ecosystem services and pollinator health. Here, we studied how urbanization affects the functional traits of workers in two bumblebee species (Bombus terrestrisandB. pascuorum) sampled in 37 sites along a gradient of urbanization in North Italy (an area of 1800 km2including the metropolitan context of Milan and other surrounding capital districts). Namely, we investigated the effect of land use composition, configuration, air temperature, flower resource abundance, and air pollutants on the variation of traits related to flight performance and of stress during insect development (i.e., wing size, wing shape and size fluctuating asymmetry). The functional traits of the two bumblebees responded idiosyncratically to urbanization. Urban temperatures were associated with smaller wing sizes inB. pascuorumand with more accentuated fluctuating asymmetry of wing size inB. terrestris. Moreover, flower abundance correlated with bigger wings inB. terrestrisand with less asymmetric wing size inB. pascuorum. Other traits did not vary significantly, and other urban variables played minor effects. These species-specific variation patterns highlight that environmental stressor linked to urbanization negatively impact the traits related to flight performance and development stability of these syntopic bumblebees, with possible consequences on the pollination service they provide.
Approximation Algorithms for Replenishment Problems with Fixed Turnover Times
Authors: Bosman Thomas , van Ee Martijn , Jiao Yang , Marchetti-Spaccamela Alberto , Ravi R. , Stougie Leen 
Published: May 2022

AbstractWe introduce and study a class of optimization problems we call replenishment problems with fixed turnover times: a very natural model that has received little attention in the literature. Clients with capacity for storing a certain commodity are located at various places; at each client the commodity depletes within a certain time, the turnover time, which is constant but can vary between locations. Clients should never run empty. The natural feature that makes this problem interesting is that we may schedule a replenishment (well) before a client becomes empty, but then the next replenishment will be due earlier also. This added workload needs to be balanced against the cost of routing vehicles to do the replenishments. In this paper, we focus on the aspect of minimizing routing costs. However, the framework of recurring tasks, in which the next job of a task must be done within a fixed amount of time after the previous one is much more general and gives an adequate model for many practical situations. Note that our problem has an infinite time horizon. However, it can be fully characterized by a compact input, containing only the location of each client and a turnover time. This makes determining its computational complexity highly challenging and indeed it remains essentially unresolved. We study the problem for two objectives:min–avg minimizes the average tour cost andmin–max minimizes the maximum tour cost over all days. Formin–max we derive a logarithmic factor approximation for the problem on general metrics and a 6-approximation for the problem on trees, for which we have a proof of NP-hardness. Formin–avg we present a logarithmic factor approximation on general metrics, a 2-approximation for trees, and a pseudopolynomial time algorithm for the line. Many intriguing problems remain open.
Students’ conceptions of work and the understanding of the economic value of labor: a developmental study of unemployment and job precarity in times of an economic crisis
Authors: Alonso Lorena , Kohen Raquel C. 
Published: May 2022

AbstractIn the aftermath of the financial and economic recession of 2008, 130 Spanish students of five age groups (8 to 17 years) and two socioeconomic backgrounds were individually interviewed about unemployment and lower wages. The participants were presented with two hypothetical situations, and their responses were qualitatively and quantitatively analyzed. The results indicated that children bring their conceptions of work when attempting to explain those phenomena. Specifically, it was found that the explanations were closely associated with the comprehension of the notion of surplus value (i.e., the idea that workers create more value than the cost of their salaries), and that the development of this notion proceeds in three levels. Younger children were simply unaware of the capacity of workers to create value (Level 1), which gave rise to some alternative conceptions (e.g., the idea that money for salaries comes from external sources). The children within the age range of 12–13 began to understand that workers create some value (Level 2). At this level, however, the notion of surplus value was not fully understood, and the idea that money for salaries comes from external sources persisted in a variety of ways. From the ages of 13–14, the children begin to understand that a wage is the price of labor and that the employer earns a profit because workers create more value than the cost of their wages (Level 3). The article presents a discussion of the possible reasons behind the slow progress observed and highlights some suggestions for educational practice.
Patterns of Service Use in Intensive Case Management: A Six Year Longitudinal Study
Authors: Golay Philippe , Bonsack Charles , Silva Benedetta , Pauli Guillaume , de Boer Eva , Morandi Stéphane 
Published: May 2022

AbstractAn Intensive Case Management (ICM) intervention has been developed in Lausanne, Switzerland. It aims to promote access to care for people with severe mental disorders who have difficulties to engage with mental health services because of the severity of their disorders and/or their marginality. ICM embrace components of assertive community treatment and critical time intervention. It is time limited and focused on critical phases of recovery. The goal of this study was to examine the heterogeneity of service use patterns of people who required ICM interventions and identify differences in patterns of duration and timing of the intervention. Records of 471 patients from the Department of Psychiatry of Lausanne University Hospital for whom the ICM team intervention was requested were analysed over a 6 year period with discrete sequential-state analysis. Trajectories could be split between six meaningful clusters including service light use and critical time intervention (58.0%), transition to long-term regular ambulatory-care (11.3%), partial transition to ambulatory care (14.4%), alternative to hospitalization (10.4%), continued ICM (4.9%) and long hospital stays (1.1%). Diagnoses of substance abuse were overrepresented among heavy users and diagnoses of schizophrenia were the most frequent diagnostic overall. Profiles of service use for ICM patients were very diverse. Long term interventions were frequently not necessary. A time-limited intervention was likely sufficient to stabilize the situation and/or engage the patient in care. A small number of situations required a sustained and long-term investment and did not always allowed for a reduction in the need for hospitalization. A general reflection on alternatives to hospitalization must be pursued, in particular for these patients.
Decoupling of silica, nitrogen and phosphorus cycling in a meromictic subalpine lake (Lake Iseo, Italy)
Authors: Scibona Alessandro , Nizzoli Daniele , Hupfer Michael , Valerio Giulia , Pilotti Marco , Viaroli Pierluigi 
Published: May 2022

AbstractSilica (Si), nitrogen (N) and phosphorus (P) loads and stoichiometry are key factors controlling the trophic status of lakes and coastal seas. In the hydrographic network, lakes also act as biogeochemical reactors, controlling both nutrient retention and fluxes. This work aimed to examine the coupling of Si, N and P cycling, together with their stoichiometry in a deep meromictic subalpine lake (Lake Iseo, Northern Italy). Si, N and P mass budgets were calculated by quantifying loads in the inlets and in the outlet over a period of 30 months (May 2016−October 2018), in-lake sedimentation rates and net nutrients accumulation in the water body. Lake Iseo acts as a biogeochemical filter, which differentially retains the external Si, N and P loads. Retention of Si and P was similar (75–79%), but considerably higher than N (45%), evidencing a decoupling of their fate due to in-lake processes. This differential retention is likely to be exacerbated by meromixis which enhances Si and P accumulation in the monimolimnion, while impairing denitrification, thus limiting N removal. Such decoupling resulted in an increase of the N:Si and N:P ratios in both the epilimnion and in the outlet compared to the inlets, whereas the ratios decreased in the monimolimnion. As a result, there may be a stronger Si and P limitation of the photic zone, leading to a shift towards more oligotrophic conditions. This transient equilibrium could be impaired in the case of water overturn produced by extreme climate events—a highly relevant issue, considering that a growing number of deep lakes are turning from holo-oligomictic to meromictic as a result of combined eutrophication and climate change.
Numerical effects on the simulation of surfactant flooding for enhanced oil recovery
Authors: Akinyele Olaitan , Stephen Karl D. 
Published: May 2022

AbstractNumerical simulation of surfactant flooding using conventional reservoir simulation models can lead to unreliable forecasts and bad decisions due to the appearance of numerical effects. The simulations give approximate solutions to systems of nonlinear partial differential equations describing the physical behavior of surfactant flooding by combining multiphase flow in porous media with surfactant transport. The approximations are made by discretization of time and space which can lead to spurious pulses or deviations in the model outcome. In this work, the black oil model was simulated using the decoupled implicit method for various conditions of reservoir scale models to investigate behavior in comparison with the analytical solution obtained from fractional flow theory. We investigated changes to cell size and time step as well as the properties of the surfactant and how it affects miscibility and flow. The main aim of this study was to understand pulse like behavior in the water bank, which we report for the first time, Our aim was to identify their cause and associated conditions. The pulses are induced by a sharp change in relative permeability as the interfacial tension changes. Pulses are diminished when adsorption is modeled, and ranged from 0.0002 kg/kg to 0.0005 kg/kg. The pulses are absent for high-resolution model of 5000 cells in x direction with a typical cell size as used in well-scale models. The growth or dampening of these pulses may vary from case to case but in this instance was a result of the combined impact of relative mobility, numerical dispersion, interfacial tension and miscibility. Oil recovery under the numerical problems reduced the performance of the flood, due to large amounts of pulses produced. Thus, it is important to improve existing models and use appropriate guidelines to stop oscillations and remove errors.
Regulation of Location-Specific Externalities from Small-Scale Polluters
Authors: Amundsen Eirik S. , Hansen Lars Gårn , Whitta-Jacobsen Hans Jørgen 
Published: May 2022

AbstractEmission damages caused by small-scale polluters such as farms, vehicles, homes and small businesses are often location-specific and such polluters are often regulated through a combination of location-differentiated cleaner technology standards and uniform, ʻdirtyʼ input regulation. We investigate how such regulations should be designed and combined under realistic assumptions. We find that if the available cleaner technologies are ‘emission capturing’ (e.g., end-of-pipe filters), they should be encouraged in both high and low damage areas, while if they are ‘input displacing’ (i.e., facilitating replacement of dirty input by cleaner input), they should be encouraged in high damage areas, but discouraged in low damage areas. Dirty input use should always be discouraged and the optimal regulation intensity may be substantial, particularly if the available cleaner technologies are input displacing.
Healthcare professionals’ perspectives on implementation of universal tumor DNA testing in ovarian cancer patients: multidisciplinary focus groups
Authors: Witjes Vera M. , Braspenning Jozé C. C. , Hoogerbrugge Nicoline , Smolders Yvonne H. C. M. , Hermkens Dorien M. A. , Mourits Marian J. E. , Ligtenberg Marjolijn J. L. , Ausems Margreet G. E. M. , de Hullu Joanne A. 
Published: May 2022

AbstractUniversal tumor DNA testing in epithelial ovarian cancer patients can function not only as an efficient prescreen for hereditary cancer testing, but may also guide treatment choices. This innovation, introduced as Tumor-First workflow, offers great opportunities, but ensuring optimal multidisciplinary collaboration is a challenge. We investigated factors that were relevant and important for large-scale implementation. In three multidisciplinary online focus groups, healthcare professionals (gynecologic oncologists, pathologists, clinical geneticists, and clinical laboratory specialists) were interviewed on factors critical for the implementation of the Tumor-First workflow. Recordings were transcribed for analysis in Atlas.ti according to the framework of Flottorp that categorizes seven implementation domains. Healthcare professionals from all disciplines endorse implementation of the Tumor-First workflow, but more detailed standardization and advice regarding the logistics of the workflow were needed. Healthcare professionals explored ways to stay informed about the different phases of the workflow and the results. They emphasized the importance of including all epithelial ovarian cancer patients in the workflow and monitoring this inclusion. Overall, healthcare professionals would appreciate supporting material for the implementation of the Tumor-First workflow in the daily work routine. Focus group discussions have revealed factors for developing a tailored implementation strategy for the Tumor-First workflow in order to optimize care for epithelial ovarian cancer patients. Future innovations affecting multidisciplinary oncology teams including clinical geneticists can benefit from the lessons learned.
Prior exposure of a fungal parasite to cyanobacterial extracts does not impair infection of its Daphnia host
Authors: Manzi Florent , Agha Ramsy , Mühlenhaupt Max , Wolinska Justyna 
Published: May 2022

AbstractCyanobacteria periodically dominate phytoplankton composition in lakes, and produce a wide array of toxic secondary metabolites. Blooms of cyanobacteria often coincide with infections of zooplankton by microparasites (such as Metschnikowia bicuspidata, a parasitic yeast of Daphnia), and prior research has shown that cyanobacteria-based diets could mitigate fungal infections of the host. Here, we tested whether cyanotoxins could exert detrimental effects against free-living parasite stages: we inoculated two genotypes of the host Daphnia galeata × longispina with fungal spores, which were previously exposed to cyanobacterial extracts or to a placebo solution. Additionally, to test for interactive effects of cyanotoxins through environmental exposure and host consumption, Daphnia from each treatment were fed using either green algae or the same cyanobacterium. Exposing spores to cyanobacterial extracts did not reduce their infectivity; instead, parasite infectivity was increased, but only on one host genotype. The effect of host diet on parasite growth was also host-genotype dependent, with only one Daphnia genotype showing impaired spore production under a toxic diet. Our results suggest that dissolved cyanobacterial compounds released during blooms may not exert any detrimental effect on fungal spore banks, but likely influence transmission of the parasite when incorporated as part of the host’s diet.
The potential influence of high uric acid exposure on surface and corrosion susceptibility of pure titanium
Authors: Liu Yao , Zhang Wen-si , Tang Ze-hua , Zhang Song-mei , Qiu Jing 
Published: May 2022

AbstractThis study investigated the corrosion susceptibility of pure titanium under uric acid exposure for 7 days based on surface analysis. The prepared pure titanium specimens, exposed to different concentrations of uric acid, were examined for surface microstructure, surface element composition and surface wettability using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and static contact angle measurement, respectively. The corrosion behaviors of titanium specimens were measured by open-circuit potential (OCP), electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization. The titanium ion release from the prepared specimens, which were immersed in Hank’s balanced salt solution (HBSS) containing different amount of uric acid, was measured by inductively coupled plasma atomic emission spectrometry (ICP-AES). More irregular pitting holes were observed on titanium surfaces exposed to a high concentration of uric acid, and XPS analyses revealed that the amount of titanium dioxide (TiO2) decreased. Titanium surfaces pre-treated with high uric acid became more hydrophobic. Furthermore, the results of OCP and potentiodynamic polarization tests showed increased corrosion susceptibility of titanium samples, while EIS data indicated more active corrosion behavior of titanium materials. The high concentration of uric acid also induced titanium ion release. High concentration of uric acid negatively influenced the surface characteristics and corrosion properties of titanium materials, which destroyed the titanium oxide film barrier. High uric acid exposure increased corrosion susceptibility of pure titanium specimens and accelerated titanium ion release.
Green & Sensitive pH-dependent Spectrofluorimetric Assay of Tamsulosin Hydrochloride and Tadalafil in their New Combined Formulation for Benign Prostatic Hyperplasia: Application to Spiked Human Plasma
Authors: Abdel Moneim Mona M. 
Published: May 2022

AbstractSensitive and green spectrofluorimetric methods were utilized for Tamsulosin Hydrochloride (TAM) and Tadalafil (TDL) assessment in bulk and their newly available combined mixture for benign prostatic hyperplasia and erectile dysfunction. The technique relies on measuring native fluorescence of TAM in 0.1 N HCl at 324 nm and TDL in 0.1 N NaOH at 348 nm due to their different fluorimetric behavior in acidic and basic media where TAM has no fluorescence in basic medium and vice versa. To achieve better regression, the spectra were derivatized allowing determination of TAM at 314 nm and TDL at 320 and 380 nm (peak to peak) by applying third and first derivative, respectively. In addition, pH-dependent “constant-wavelength synchronous” spectrofluorimetry was applied where TAM and TDL were determined at 218 nm in acidic medium and at 268 nm in basic medium, respectively. Finally, derivatizing the latter emission spectra allowed determination of TAM and TDL at 232 nm and at 262 and 278 nm (peak to peak), respectively. Acidic and basic emission spectra where scanned at λexc = 225 nm (for TAM assay) and at λexc = 247 nm (for TDL assay), respectively. Fluorescence–concentration plots were linear and the proposed methods were used for analysis of TAM and TDL combined laboratory prepared formulation. These procedures are green, sensitive and of low cost which make them suitable for quality control analysis of the two drugs. In addition, the high selectivity of the proposed methods was tested by successfully applying them for TAM and TDL assay in plasma samples.
Experimental Procedure to Assess Depth and Size of Defects with Pulsed Thermography
Authors: D’Accardi Ester , Palumbo Davide , Galietti Umberto 
Published: May 2022

AbstractSubsurface defects can be detected by the pulsed thermography (PT) technique analysing the raw thermal data with the application of different post-processing algorithms. In this regard, different methods, based on one-dimensional models, are used in the literature to estimate the depth and size of defects. Two of the most established methods are the thermal signal reconstruction (TSR) and the pulsed phase thermography (PPT) algorithms. These latter require a careful set up of the testing parameters such as the frame rate, the truncation window size, and the energy density to obtain an accurate estimation of both depths and sizes of defects. Even if some works have already investigated the issue of defect characterization, there are few works in which the correct procedures to obtain both the size and depth were deeply explained, above all for real components with real defects. The aim of this work is to propose a new empirical procedure to obtain depth and size estimation of the defects using the pulsed thermography technique and in particular the principal component thermography (PCT) algorithm. The proposed procedure is based on the experimental observation that exists a linear correlation between the defect contrasts and the relative aspect ratios. In this way, by means of a master specimen, a calibration curve can be obtained considering a suitable truncation window of the analysed data. Then, the size and depth of defects have been retrieved imposing threshold criteria. The procedure is quite general, and it can be also tested with other algorithms. Different experimental tests have been carried out on two materials, aluminium and glass fiber reinforced polymer (GFRP) and then the procedure has been applied and validated both on simulated (flat bottom holes) and real defects.