bluuebunny/crossref_metadata_2025_split huggingface dataset:
Syntheticin vivocompartmentalisation improves metabolic flux and modulates the product profile of promiscuous enzymes
Authors: Cheah Li Chen , Liu Lian , Plan Manuel R. , Peng Bingyin , Lu Zeyu , Schenk Gerhard , Vickers Claudia E. , Sainsbury Frank 
Published: November 2022

ABSTRACTEnzyme spatial organisation and compartmentalisation are naturally evolved mechanisms for facilitating multi-step biocatalysis. We explored the syntheticin vivoco-encapsulation of two different cargo proteins in yeast using a self-assembling virus-like particle. Co-encapsulation was verified using single particle techniques for both end-to-end fusion of the cargo proteins with the encapsulation anchor at one end, and coexpression of each cargo protein with their individual anchors. The co-encapsulation of a bifunctional geranyl diphosphate/farnesyl diphosphate synthase and a bifunctional linalool/nerolidol synthase delivered nerolidol titres up to 30 times that of an unorganised ‘free’ enzyme control, a remarkable improvement from a single engineering step. Interestingly, striking differences in the ratio of products (linalool and nerolidol) were observed with each spatial organisation approach. This work presents the largest reported titre fold increases fromin vivoenzyme compartmentalisation and suggests that enzyme spatial organisation could be used to modulate the product profile of promiscuous enzymes.
The impact of dance movement interventions on psychological health in older adults without dementia: A systematic review and meta-analysis
Authors: Podolski Odile , Whitfield Tim , Schaaf Leah , Cornaro Clara , Köbe Theresa , Koch Sabine , Wirth Miranka 
Published: November 2022

AbstractMultimodal lifestyle-based interventions that integrate physical, mental and social stimulation could promote mental health and brain resilience against dementia. This meta-analysis examined the efficacy of dance movement interventions (DMI) on psychological health in older adults. Pre-registration was done with PROSPERO (CRD42021265112). PubMed, Web of Science and PsycInfo were searched for randomized controlled trials (RCT) evaluating the effects of DMI (>4 weeks’ duration) on measures of psychological health (primary outcome) and cognitive function (additional outcome) among older adults without dementia (≥55). Data of 13 primary RCT (n=943,n-DMI=474,n-control=469) were synthesized using a random effects meta-analysis with robust variance estimation. DMI had a small positive effect on overall psychological health (g=0.31;95% CI: [0.09,0.53];p=.01,I2=62.55) and a medium effect on general cognitive function (g=0.48;95% CI: [0.03,0.93],p=.04,I2=82.45) compared to comparators. None of the primary studies included measures of neuroplasticity. DMI may serve as a multimodal enrichment strategy to promote healthy mental aging. High-quality intervention studies are needed to expand evidence for psychological domains and identify the underlying neurophysiological correlates.
Visualization of Incrementally Learned Projection Trajectories for Longitudinal Data
Authors: Malepathirana Tamasha , Senanayake Damith , Gautam Vini , Engel Martin , Balez Rachelle , Lovelace Michael D. , Sundaram Gayathri , Heng Benjamin , Chow Sharron , Marquis Chris , Guillemin Gilles , Brew Bruce , Jagadish Chennupati , Ooi Lezanne , Halgamuge Saman 
Published: November 2022

ABSTRACTLongitudinal studies that continuously generate data enable the capture of temporal variations in experimentally observed parameters, facilitating the interpretation of results in a time-aware manner. We propose IL-VIS (Incrementally Learned Visualizer), a new machine learning pipeline that incrementally learns and visualizes a progression trajectory representing the longitudinal changes in longitudinal studies. At each sampling time point in an experiment, IL-VIS generates a snapshot of the longitudinal process on the data observed thus far, a new feature that is beyond the reach of classical static models. We first verify the utility and correctness of IL-VIS using simulated data, for which the true progression trajectories are known. We find that it accurately captures and visualizes the trends and (dis)similarities between high-dimensional progression trajectories. We then apply IL-VIS to longitudinal Multi-Electrode Array data from brain cortical organoids when exposed to different levels of Quinolinic Acid, a metabolite contributing to many neuroinflammatory diseases including Alzheimer’s disease, and its blocking antibody. We uncover valuable insights into the organoids’ electrophysiological maturation and response patterns over time under these conditions.
Fast actin disassembly and fimbrin mechanosensitivity support rapid turnover during clathrin-mediated endocytosis
Authors: Mousavi Sayed Iman , Lacy Michael M. , Li Xiaobai , Berro Julien 
Published: November 2022

AbstractThe actin cytoskeleton is central to force production in numerous cellular processes in eukaryotic cells. During clathrin-mediated endocytosis (CME), a dynamic actin meshwork is required to deform the membrane against high membrane tension or turgor pressure. Previous experimental work from our lab showed that several endocytic proteins, including actin and actin-interacting proteins, turn over several times during the formation of a vesicle during CME in yeast, and their dwell time distributions were reminiscent of Gamma distributions with a peak around 1 s (Lacy et al., 2019). However, the distribution for the filament crosslinking protein fimbrin contains a second peak around 0.5 s. To better understand the nature of these dwell time distributions, we developed a stochastic model for the dynamics of actin and its binding partners. Our model demonstrates that very fast actin filament disassembly is necessary to reproduce experimental dwell time distributions. Our model also predicts that actin-binding proteins bind rapidly to nascent filaments and filaments are fully decorated. Last, our model predicts that fimbrin detachment from actin endocytic structures is mechanosensitive to explain the extra peak observed in the dwell time distribution.
Analysing the marketing strategies that fish farming businesses in the UK can use to gain a competitive advantage
Authors: Olawunmi Christiana Adeola , Clarke Andrew Paul 
Published: November 2022

Purpose
This study aims to explore marketing strategies that UK fish farming businesses can use to gain a competitive advantage. The marketing strategies examined include product branding and core competencies, sales promotion, market positioning and segmentation.

Design/methodology/approach
A survey through an online questionnaire was mailed to five randomly selected trade associations of UK fish farming businesses and distributed to their registered members, of which 200 responded. Both male and female genders with different age groups and levels of experience in the UK fish farming business participated. In addition, ten articles were sampled for a systematic review.

Findings
Results show that UK fish farming businesses could increase sales by using ecolabels in product branding to attract premium prices, build consumer confidence and using high-quality packages for fish products will keep fish fresh for a longer period.

Research limitations/implications
The scope of this research is limited to the UK. The findings cannot be generalised and used for other jurisdictions because of variable economic and market conditions.

Originality/value
A significant recommendation from this case study is that fish farming businesses need to be creative and innovative in ways such as leveraging branding, sales promotions and core competencies to win the trust and confidence of consumers. Most importantly, each fish farming business should know the specific marketing strategy that works for them; this case study shows that not all branding and sales promotion techniques enhance competitiveness. The scope of this research is limited to the UK. The findings cannot be generalised and used for other jurisdictions because of variable economic and market conditions.
Clinical course and outcomes of pemphigus vulgaris and foliaceus: A retrospective study using a nationwide database in Japan
Authors: Miyachi Hideaki , Konishi Takaaki , Hashimoto Yohei , Matsui Hiroki , Fushimi Kiyohide , Inozume Takashi , Yasunaga Hideo 
Published: November 2022

AbstractPemphigus is a life‐threatening autoimmune blistering disease. Patient characteristics, treatment courses, and outcomes remain unclear owing to its rarity. To describe the background, treatment, and outcomes of pemphigus, we identified 2598 patients with pemphigus vulgaris and 1186 patients with pemphigus foliaceus from a nationwide inpatient database in Japan. Patients with pemphigus vulgaris were younger (62 vs 72 years, P < 0.001), had fewer comorbidities, and were more likely to be admitted to high‐volume hospitals (38% vs 30%, P < 0.001) than those with pemphigus foliaceus. Patients with pemphigus vulgaris had undergone more aggressive treatment, including steroid pulse therapy, intravenous immunoglobulin, or plasmapheresis, compared with those with pemphigus foliaceus (48% vs 42%, P = 0.001); specifically, in patients aged <70 years, the pemphigus vulgaris group was more likely to undergo aggressive treatment than the pemphigus foliaceus group (52% vs 45%), whereas there was no significant difference in patients aged ≥70 years (40% vs 40%). Immunosuppressive agents (30% vs 26%, P = 0.015) and analgesics, including opioids (45% vs 36%, P < 0.001), were used more frequently, whereas topical corticosteroids were used less frequently (32% vs 48%, P < 0.001) in patients with pemphigus vulgaris compared with those with pemphigus foliaceus. In‐hospital mortality was lower in patients with pemphigus vulgaris than in those with pemphigus foliaceus (2.2% vs 4.0%, P = 0.002); in the comparison stratified by age, the mortality was equivalent among the two groups (0.6% in patients aged <70 years and 6.1% in those aged ≥70 years). Overall, patients with pemphigus vulgaris had a 10‐day longer hospitalization period and higher hospitalization costs than those with pemphigus foliaceus. Our findings provide useful information for understanding the current trends in the management of pemphigus in Japan.
Full waveform inversion based on inversion network reparameterized velocity
Authors: Jiang Peng , Wang Qingyang , Ren Yuxiao , Yang Senlin , Li Ningbo 
Published: November 2022

AbstractSeismic velocity plays an important role in imaging and identifying underground geology. Conventional seismic velocity inversion methods, like full waveform inversion, directly update the velocity model based on the misfit between the observed and synthetic data. However, seismic velocity inversion is a highly nonlinear process, and the inversion effect greatly relies on the initial inversion model. In this paper, we propose a novel network‐domain full waveform inversion method. Different from the existing network‐domain full waveform inversion methods, which use random or fixed numbers as network input, we reparameterize the low‐dimensional acoustic velocity model in a high‐dimensional inversion network parameter domain with seismic observed data as the network input. In this way, the physical information within the observed data can be directly encoded into the inversion parameters, leading to a better inversion effect than the current network‐domain full waveform inversion method. Moreover, comparison experiments on the Society of Exploration Geophysicists and the European Association of Geoscientists and Engineers Overthrust model and the Marmousi model show the advantages of the proposed method over conventional full waveform inversion from the aspects of inversion accuracy, robustness to noisy data, and more complex geological structures. These advantages may benefit from the fact that reparameterization within the inversion network domain can empower the inversion process with the regularization ability of denoising and mitigating the cycle‐skipping issue. In the end, the potential of the proposed method in terms of network initialization is further discussed.
Inversion of self‐potential source based on particle swarm optimization
Authors: Luo Yijian , Du Xingzhong , Cui Yian , Guo Youjun , Xie Jing , Liu Jianxin 
Published: November 2022

AbstractParticle swarm optimization is widely applied in self‐potential inversion, but most of these applications are limited to simple polarized bodies like inclined sheets and spheres. In this paper, two variants of particle swarm optimization are formed by introducing the resistivity constraint matrix and the damping factor and are then applied to two‐dimensional self‐potential source inversion. The two variants are combined to balance the exploration and exploitation capabilities in different situations, and the rationality of the parameter selection is validated through the stability analysis. To ensure both compactness and smoothness of the inversion results, we adopt an objective function based on L1–L2 norm regularization. Finally, we verify the effectiveness of the proposed algorithm through synthetic examples, the Cu–Fe model sandbox experiment and a field example. By imposing certain constraints, particle swarm optimization also shows great potential in high‐dimensional self‐potential source inversion, instead of being limited to the inversion of a few parameters.
Epidermal growth factor receptor tyrosine kinase inhibitors for non‐small cell lung cancer harboring uncommon EGFR mutations: Real‐world data from Taiwan
Authors: Chang John Wen‐Cheng , Huang Chen‐Yang , Fang Yueh‐Fu , Chang Ching‐Fu , Yang Cheng‐Ta , Kuo Chih‐Hsi Scott , Hsu Ping‐Chih , Wu Chiao‐En 
Published: November 2022

AbstractBackgroundEpidermal growth factor receptor tyrosine kinase inhibitors (EGFR‐TKIs) are the standard treatment for patients with non‐small cell lung cancer (NSCLC) harboring EGFR mutations. This study aimed to evaluate the efficacy of EGFR‐TKIs and prognostic factors for patients with NSCLC harboring uncommon EGFR mutations, which account for 10% of EGFR mutations.MethodsA total of 230 treatment‐naive patients with NSCLC harboring uncommon EGFR mutations treated with first‐line EGFR‐TKIs between 2011 and 2018 at four hospitals (belonging to four institutions, Linkou, Kaohsiung, Keelung, and Chiayi, of the Chang Gung Memorial Hospital) in Taiwan were retrospectively reviewed. Their clinicopathological characteristics, adverse events (AEs), objective response rate (ORR), disease control rate (DCR), progression‐free survival (PFS), and overall survival (OS) were collected. Univariate and multivariate analyses were performed to identify potential prognostic factors for PFS.ResultsOverall, patients who received afatinib (n = 62) had better PFS (median: 6.4 vs. 5.9 months, p = 0.022) and OS (median: 13.4 vs. 13.0 months, p = 0.008) than those who received gefitinib/erlotinib (n = 124), although no significant differences were observed for ORR (46.8% vs. 35.5%, p = 0.137) or DCR (59.7% vs. 58.9%, p = 0.916). Patients who received afatinib showed significantly higher ORR (58.3% vs. 31.3%, p = 0.027) but not DCR compared with gefitinib/erlotinib for major uncommon mutations. Afatinib trended toward better PFS and OS for major uncommon mutations and compound mutations. No EGFR‐TKIs were effective for most NSCLC patients with exon 20 insertions. Performance status, metastasis of the liver and pleura, and dose reduction were independent prognostic factors for PFS.ConclusionAfatinib demonstrated better survival outcomes than gefitinib/erlotinib for NSCLC patients harboring major EGFR uncommon mutations and compound mutations. Performance status and metastatic sites may be useful for predicting PFS for major uncommon mutations and compound mutations.
Follicle‐stimulating hormone effectiveness in male idiopathic infertility: What happens in daily practice?
Authors: Romeo Marilina , Spaggiari Giorgia , Nuzzo Federico , Granata Antonio R. M. , Simoni Manuela , Santi Daniele 
Published: November 2022

AbstractObjectivesTo assess the effectiveness of follicle‐stimulating hormone (FSH) administration in male idiopathic infertility in a clinical setting.MethodsA retrospective real‐world study was carried out, including all consecutive FSH‐treated infertile men attending the Andrology Unit of Modena (Italy) from June 2015 to May 2022. Medical history, physical and andrological examinations, hormonal and seminal parameters, therapeutic management and pregnancy data were collected. The primary endpoint was the number of pregnancies obtained after FSH administration, whereas semen parameters change was the secondary outcome.ResultsA total of 194 of 362 (53.6%) infertile men, eligible according to the Italian Health System regulations, were treated with FSH (mean age 37.9 ± 6.1 years). Following FSH administration (mean therapy duration 9.1 ± 7.1 months), 43 pregnancies were recorded (27.6%), of which 22 occurred naturally and 21 after assisted reproduction. A significant increase in sperm concentration (9.9 ± 12.2 vs. 18.9 ± 38.9 million/mL, p = 0.045) was detected after treatment, together with a significant increase in normozoospermia (from 1.0% to 5.1%, p = .044) and a reduction in azoospermia rate (from 9.8% to 7%, p = 0.044). Dividing the cohort in FSH‐responders and non‐responders, in terms of pregnancy achieved, higher sperm concentrations (15.7 ± 26.6 vs. 22.2 ± 25.7 million/mL, p = 0.033) and progressive sperm motility (18.0 ± 18.2 vs. 27.3 ± 11.3, p = 0.044) were found in pregnancy group.ConclusionOur experience suggests that FSH, empirically administered to men with idiopathic infertility, leads to pregnancy in one out of four patients and increases sperm concentration. Although the expected limits because of a real‐world data study, the number of FSH‐treated patients required to achieve one pregnancy seems to be lower in clinical setting if compared to previously published data.
A critical review of the precautionary approach of the IUCN impact classification for non‐native taxa
Authors: Cassini Marcelo H. 
Published: November 2022

AbstractThe International Union for the Conservation of Nature (IUCN) proposes the use of the Environmental Impact Classification for Alien Taxa to standardize the classification of introduced species (IS) based on their environmental impact. The IUCN invoked the precautionary principle (PP) via 2 rules: the impact assigned to a taxon must be the maximum recorded impact across different impact assessments, and when the main driver of environmental damage is unclear, it must be assumed to be caused by the IS. The validity of PP is conditioned on the degree of emergency that warrants urgent decisions and on the scientific evidence demonstrating the advantages of applying a preventive measure. The application of an impact classification system does not arise in the context of an emergency that requires management; it occurs before the decision‐making phase. Thus, PP should not be used in early steps of the risk analysis process. The IUCN also did not provide enough scientific basis to justify the use of PP. Instead, the PP rules appear to be rooted primarily in the ethical value system underlying conservation science. Conservationists assign intrinsic value to native species by virtue of their roles and relationships within ecological and evolutionary systems and processes; thus, individuals introduced in new environments not only cease to have value because they are no longer part of that natural diversity and lack those links with the rest of the ecosystem, but they become a threat to what conservationists value most. The consequence of this belief is that all introduced taxa will have an impact at some level, suggesting that values justify the PP rules.
Burden of migraine in Brazil: A cross‐sectional real‐world study
Authors: Souza Marcio Nattan Portes , Cohen Joshua M. , Piha Tony , Ribalov Rinat , Lengil Tamar , van der Laan Andressa , Calderaro Marcelo , Lee Lulu K. 
Published: November 2022

AbstractObjectiveTo assess the burden and consequences of migraine in Brazil in terms of health‐related quality of life (HRQoL), work productivity and daily activities, and healthcare resource utilization (HRU).BackgroundDespite existing data on how migraine affects populations worldwide, there are limited data on the burden of migraine in Latin America.MethodsThis cross‐sectional study used patient‐reported data from the 2018 Brazil National Health and Wellness Survey. HRQoL scores (EuroQol 5‐dimension 5‐level [EQ‐5D‐5L]; 36‐item Short Form Health Survey, version 2 [SF‐36v2]; and Short Form 6‐dimension [SF‐6D]), impairments to work productivity and daily activities (Work Productivity and Activity Impairment questionnaire), and all‐cause HRU were compared between migraine respondents and matched non‐migraine controls.ResultsOf the 12,000 total respondents in the survey database, 1643 self‐reported a physician diagnosis of migraine and were propensity score matched 1:1 with controls without migraine. HRQoL was lower in patients with migraine versus non‐migraine controls, with significantly lower SF‐36v2 physical (mean [± SD] 50.3 [7.5] vs. 52.0 [7.6]) and mental component (mean [± SD] 42.9 [10.2] vs. 46.0 [9.9]) summary scores and SF‐6D (mean [± SD] 0.7 [0.1] vs. 0.7 [0.1]) and EQ‐5D‐5L (mean [± SD] 0.7 [0.2] vs. 0.8 [0.2]) utility scores (all p < 0.001). Patients with migraine reported higher levels of work productivity loss (mean [± SD], 40.6% [31.4%] vs. 28.6% [30.9%], including absenteeism 12.8% [19.1%] vs. 8.4% [17.1%] and presenteeism 35.0% [28.7%] vs. 24.8% [28.0%]; all p < 0.001); activity impairment (mean [± SD] 36.0% [28.8%] vs. 25.5% [28.1%]; p < 0.001); and significantly higher HRU in the past 6 months (healthcare provider and emergency department visits [mean [± SD] 7.2 [9.5] vs. 4.5 [6.3] and 1.7 [3.8] vs. 0.9 [2.2]; both p < 0.001] and hospitalizations [mean [± SD] 0.4 [2.7] vs. 0.2 [1.1]; p = 0.002]) than controls.ConclusionMigraine is associated with poorer HRQoL, higher all‐cause HRU, and greater activity impairment and work productivity loss versus non‐migraine controls in Brazil.
B4C reinforced NiTi‐based composites: Microstructure and wear performance
Authors: Velayatipour Omid , Nikzad Leila , Farvizi Mohammad 
Published: November 2022

AbstractIn this study, NiTi–x wt.% B4C (x = 0, 2, and 4) composites were consolidated with spark plasma sintering method, and the effects of boron carbide reinforcement addition on the microstructure and wear behavior of samples were investigated. Identification of the constituent phases of samples by the X‐ray diffraction method plus Rietveld analysis revealed that the stability of the martensite phase increased in the composite samples because of mismatch stresses between the NiTi matrix phase and the reinforcing particles, which increases the density of the dislocations and facilitates the diffusion process that subsequently leads to the formation of stable intermetallics. The results of hardness test indicated that the hardness value increased from 3.67 GPa for pure NiTi to 10.99 GPa for NiTi–4 wt.% B4C. Results of wear test revealed that boron carbide reinforced composite specimens had higher wear resistance, whereas wear rate of NiTi sample was 3.6 × 10−3 mm3/N m, and it reached to .21 × 10−3 mm3/N m for NiTi–4 wt.% B4C. Investigation of microstructure by scanning electron microscopy images and EDS analysis revealed that the wear mechanism in NiTi samples was abrasive and the addition of B4C to NiTi changed the wear mechanisms from abrasive to a combination of oxidation, adhesive, and delamination mechanisms.