bluuebunny/crossref_metadata_2025_split huggingface dataset:
Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis
Authors: Aldholmi Mohammed , Ahmad Rizwan , Carretero‐Molina Daniel , Pérez‐Victoria Ignacio , Martín Jesús , Reyes Fernando , Genilloud Olga , Gourbeyre Léa , Gefflaut Thierry , Carlsson Hanne , Maklakov Alexei , O'Neill Ellis , Field Robert A. , Wilkinson Barrie , O'Connell Maria , Ganesan A. 
Published: March 2022

AbstractBy limiting the nitrogen source to glutamic acid, we isolated cyclic peptides from Euglena gracilis containing asparagine and non‐proteinogenic amino acids. Structure elucidation was accomplished through spectroscopic methods, mass spectrometry and chemical degradation. The euglenatides potently inhibit pathogenic fungi and cancer cell lines e.g., euglenatide B exhibiting IC50 values of 4.3 μM in Aspergillus fumigatus and 0.29 μM in MCF‐7 breast cancer cells. In an unprecedented convergence of non‐ribosomal peptide synthetase and polyketide synthase assembly‐line biosynthesis between unicellular species and the metazoan kingdom, euglenatides bear resemblance to nemamides from Caenorhabditis elegans and inhibited both producing organisms E. gracilis and C. elegans. By molecular network analysis, we detected over forty euglenatide‐like metabolites in E. gracilis, E. sanguinea and E. mutabilis, suggesting an important biological role for these natural products.
Preparation and application of dielectric polymers with high permittivity and low energy loss: A mini review
Authors: Qiu Jie , Gu Qun , Sha Ye , Huang Yang , Zhang Meng , Luo Zhenyang 
Published: March 2022

AbstractThis article reviews the preparation strategies of dielectric polymers with high permittivity and low energy loss, as well as their applications in the field of energy storage and intelligent sensors. We discuss the dependence of dielectric properties of polymer composites on different types of dielectric polarizations, including interfacial polarization, orientational polarization, ionic polarization, and electronic polarization, as well as the improvement of dielectric properties by synergetic effects of multiple polarizations. Due to the difficulties of achieving both high dielectric polarization (hence high permittivity) and low dielectric loss, we focus on the energy loss mechanism of different polarization types and issues in the preparation process. We also discuss current applications and future prospects of dielectric polymers in the field.
Rapid Generation of Tetrahydropyridines and Tetrahydroquinolines by Dearomative Cyanation/Grignard Addition
Authors: Zheng Shixin , Wang Dong , Huang Mindong , Yu Peng 
Published: March 2022

AbstractA rapid, practical and scalable method for the reductant and tansition‐metal‐free synthesis of a variety of novel 2,4‐disubstituted tetrahydropyridines and tetrahydroquinolines is disclosed. The method is based upon dearomative functionalization of pyridines or quinolines to generate amino nitrile intermediates as masked iminium ions, which then react rapidly with various Grignard reagents in complete stereocontrol.
Light‐Controlled Ionic/Molecular Transport through Solid‐State Nanopores and Nanochannels
Authors: Lu Jiahao , Jiang Yanan , Yu Ping , Jiang Wei , Mao Lanqun 
Published: March 2022

AbstractBiological nanochannels perfectly operate in organisms and exquisitely control mass transmembrane transport for complex life process. Inspired by biological nanochannels, plenty of intelligent artificial solid‐state nanopores and nanochannels are constructed based on various materials and methods with the development of nanotechnology. Specially, the light‐controlled nanopores/nanochannels have attracted much attention due to the unique advantages in terms of that ion and molecular transport can be regulated remotely, spatially and temporally. According to the structure and function of biological ion channels, light‐controlled solid‐state nanopores/nanochannels can be divided into light‐regulated ion channels with ion gating and ion rectification functions, and light‐driven ion pumps with active ion transport property. In this review, we present a systematic overview of light‐controlled ion channels and ion pumps according to the photo‐responsive components in the system. Then, the related applications of solid‐state nanopores/nanochannels for molecular sensing, water purification and energy conversion are discussed. Finally, a brief conclusion and short outlook are offered for future development of the nanopore/nanochannel field.
The effect of high‐fidelity flow models on electromagnetic flowmeter analysis
Authors: Beck Kade J. , Barfuss Steven L. , Sharp Zachary B. , Johnson Michael C. , Moon Todd K. , Dutta Som 
Published: March 2022

AbstractThe current research is unclear regarding the sensitivity of the output of a magnetic flowmeter analysis to the fidelity of the simulated flow field. This study evaluates the effects of higher fidelity models on magnetic flowmeter analysis. An eddy viscosity model, second‐moment closure model, and a Large‐Eddy simulation were compared to laboratory velocity profile data 0.67D downstream of a 1.58D elbow at a Reynolds number of 34,000. The Large‐Eddy simulation results matched the laboratory velocity profile data best. The authors conclude that the fidelity of the flow field model does cause differences in the analysis of the flowmeter voltage output.
Computational analysis and in vitro evaluation of TMF 104, for its antioxidant, antimicrobial, and anticancer efficacies
Authors: Saif Ahmed , Al Shahrani Mesfer , Alshehri Mohammed , Abohassan Mohammad , Alshehri Mohammed A. , Radhakrishnan Suresh , Rajagopalan Prasanna 
Published: March 2022

AbstractBenzylidene chromanones are small molecules, structurally similar to active phytochemicals. Herein, we report one novel benzylidene chromanone, TMF 104, for its bio‐efficacies. Its computational docking for Vanin‐1, antioxidant, free radical scavenging capacities, antimicrobial effects, and anticancer efficacy were analyzed. TMF 104 predicated strong binging to Vanin‐1 protein with a docking energy of −8.1 kcal/mol. The compound dose‐dependently exhibited free‐radical scavenging and antioxidant activities when tested in vitro. The compound also had remarkable activity against Salmonella typhimurium, Enterococcus faecalis, Staphylococcus aureus, and Escherichia coli with minimum inhibitory concentration values of 1.5, 2.0, 12.5, and 13.5 μg/ml, respectively. The compound was also effective against Bacillus cereus and Pseudomonas aeruginosa albeit at higher concentrations. TMF 104 dose‐dependently inhibited the proliferation of MCF‐7, NCI H460, and Caki‐1 cells with respective GI50 values of 24.51, 21.95, and 32.95 μg/ml, whereas the compound was toxic to normal Vero cells at much higher concentration of 264.70 μg/ml. The compound also aided in apoptosis and increased the sub G0/G1 phase of the cell cycle in all three cancer cells tested. Our study identified a novel, potent benzylidene analogue with potent antioxidant, antimicrobial, and anticancer activities, which drives further attention for further research.
Nitrogen‐doped carbon dots as a fluorescent probe for the highly sensitive detection of bilirubin and cell imaging
Authors: Li Ningbo , Hu Chuqian , Zhang Wenkun , Ma Rong , Zhang Liting , Qiao Jie 
Published: March 2022

AbstractNitrogen‐doped carbon dots (NCDs) with bright blue fluorescence were constructed by a hydrothermal method using sucrose and l‐proline as raw materials. The NCDs were characterized by transmitted electron microscopy, X‐ray diffraction, Fourier‐transform infrared spectrometry, X‐ray photoelectron spectroscopy, and ultraviolet‐visible absorption and fluorescence spectroscopy to investigate the morphology, elemental composition, and optical properties. The NCDs had good water solubility, high dispersibility with an average diameter of only 1.7 nm, and satisfactory optical properties with a fluorescence quantum yield of 23.4%. The NCDs were employed for the detection of bilirubin. A good linear response of the NCDs in the range 0.35–9.78 μM was obtained for bilirubin with a detection limit of 33 nM. The NCDs were also applied to the analysis of real samples, serum and urine, with a recovery of 95.34% to 104.66%. The low cytotoxicity and good biocompatibility of the NCDs were indicated by an MTT assay and cell imaging of HeLa cells. Compared with other detection systems, using NCDs for bilirubin detection was a facile and efficient method with good selectivity and sensitivity.
A facile fluorescence turn‐on biosensor customized for monitoring of protein kinase activity based on carboxylic carbon nanoparticle–peptide complexes
Authors: Feng Tingting , Yan Shuzhu , Wang Zheng , Fan Xiaohui 
Published: March 2022

AbstractIn this study, we present a facile and low‐cost approach for detecting protein kinase A (PKA) by assembling a purpose‐designed carboxyfluorescein (FAM)‐labelled peptide with carboxylic carbon nanoparticles (CNPs). Fluorescence of the FAM‐labelled peptide gradually decreases to a low background signal as a result of the electron transfer from CNPs to FAM‐labelled peptide via the peptide, which acts as a bridge. The reaction in the sensor in the presence of adenosine 5′‐triphosphate and PKA phosphorylates the substrate peptide and disrupts the electrostatic repulsive force between the CNPs and the peptide, therefore altering the spectroscopic signal of the system. The change in fluorescence signal was directly proportional to the PKA concentration in the range 0–1.8 U/ml with a detection limit of 0.04 U/ml. These results suggest that PKA activity can be effectively measured using the developed PKA biosensor. Moreover, the fluorescence biosensor was successfully used in the investigation of PKA in spiked human embryonic kidney (HEK) 293 cells lysates, indicating its potential applications in protein kinase‐related biochemical fundamental research.
Pharmacogenomics of cisplatin‐induced neurotoxicities: Hearing loss, tinnitus, and peripheral sensory neuropathy
Authors: Zhang Xindi , Trendowski Matthew R. , Wilkinson Emma , Shahbazi Mohammad , Dinh Paul C. , Shuey Megan M. ,   Regeneron Genetics Center, Feldman Darren R. , Hamilton Robert J. , Vaughn David J. , Fung Chunkit , Kollmannsberger Christian , Huddart Robert , Martin Neil E. , Sanchez Victoria A. , Frisina Robert D. , Einhorn Lawrence H. , Cox Nancy J. , Travis Lois B. , Dolan M. Eileen 
Published: March 2022

AbstractPurposeCisplatin is a critical component of first‐line chemotherapy for several cancers, but causes peripheral sensory neuropathy, hearing loss, and tinnitus. We aimed to identify comorbidities for cisplatin‐induced neurotoxicities among large numbers of similarly treated patients without the confounding effect of cranial radiotherapy.MethodsUtilizing linear and logistic regression analyses on 1680 well‐characterized cisplatin‐treated testicular cancer survivors, we analyzed associations of hearing loss, tinnitus, and peripheral neuropathy with nongenetic comorbidities. Genome‐wide association studies and gene‐based analyses were performed on each phenotype.ResultsHearing loss, tinnitus, and peripheral neuropathy, accounting for age and cisplatin dose, were interdependent. Survivors with these neurotoxicities experienced more hypertension and poorer self‐reported health. In addition, hearing loss was positively associated with BMIs at clinical evaluation and nonwork‐related noise exposure (>5 h/week). Tinnitus was positively associated with tobacco use, hypercholesterolemia, and noise exposure. We observed positive associations between peripheral neuropathy and persistent vertigo, tobacco use, and excess alcohol consumption. Hearing loss and TXNRD1, which plays a key role in redox regulation, showed borderline significance (p = 4.2 × 10−6) in gene‐based analysis. rs62283056 in WFS1 previously found to be significantly associated with hearing loss (n = 511), was marginally significant in an independent replication cohort (p = 0.06; n = 606). Gene‐based analyses identified significant associations between tinnitus and WNT8A (p = 2.5 × 10−6), encoding a signaling protein important in germ cell tumors.ConclusionsGenetics variants in TXNRD1 and WNT8A are notable risk factors for hearing loss and tinnitus, respectively. Future studies should investigate these genes and if replicated, identify their potential impact on preventive strategies.
Different patients, different preferences: A multicenter assessment of patients' personality traits and anxiety in shared decision making
Authors: Köther Anja K. , Büdenbender Björn , Grüne Britta , Holbach Sonja , Huber Johannes , von Landenberg Nicolas , Lenk Julia , Martini Thomas , Michel Maurice S. , Kriegmair Maximilian C. , Alpers Georg W. 
Published: March 2022

AbstractObjectivePatient‐centered care and shared decision making (SDM) are generally recognized as the gold standard for medical consultations, especially for preference‐sensitive decisions. However, little is known about psychological patient characteristics that influence patient‐reported preferences. We set out to explore the role of personality and anxiety for a preference‐sensitive decision in bladder cancer patients (choice of urinary diversion, UD) and to determine if anxiety predicts patients' participation preferences.MethodsWe recruited a sample of bladder cancer patients (N = 180, primarily male, retired) who awaited a medical consultation on radical cystectomy and their choice of UD. We asked patients to fill in a set of self‐report questionnaires before this consultation, including measures of treatment preference, personality (BFI‐10), anxiety (STAI), and participation preference (API and API‐Uro), as well as sociodemographic characteristics.ResultsMost patients (79%) indicated a clear preference for one of the treatment options (44% continent UD, 34% incontinent UD). Patients who reported more conscientiousness were more likely to prefer more complex methods (continent UD). The majority (62%) preferred to delegate decision making to healthcare professionals. A substantial number of patients reported elevated anxiety (32%), and more anxiety was predictive of higher participation preference, specifically for uro‐oncological decisions (β = 0.207, p < 0.01).ConclusionsOur findings provide insight into the role of psychological patient characteristics for SDM. Aspects of personality such as conscientiousness influence treatment preferences. Anxiety contributes to patients' motivation to be involved in pertinent decisions. Thus, personality and negative affect should be considered to improve SDM.
Uptake of genetic counseling and testing in a clinic‐based population of women with breast cancer
Authors: Wehbe Alexandra , Manning Mark , Assad Hadeel , Purrington Kristen S. , Simon Michael S. 
Published: March 2022

AbstractBackgroundThe study was conducted to evaluate racial differences in referral and uptake of genetic counseling (GC) in a clinic‐based population of women with breast cancer.MethodsMedical records of 150 breast cancer patients at the Karmanos Cancer Institute were reviewed to determine eligibility for GC according to National Comprehensive Cancer Network guidelines, GC referral rates, and appointment completion rates. Logistic regression was used to assess the relationship between demographic and clinical factors and GC eligibility and referral.ResultsThe mean age at diagnosis was 57.1 (SD 12.6) and 66% of the women were Black. There were 91 women (60.7%) eligible for GC and of those, 54 (61.4%) were referred. After multivariable analyses, factors associated with reduced eligibility were older age at diagnosis (OR = 0.91, 95% CI [0.87,0.95]) and Black race (OR = 0.37, 95% CI [0.15, 0.96]). After additional multivariable analysis, eligibility was associated with an increased likelihood of referral (OR = 5.97, 95% CI [2.29, 15.56]), however, Medicare versus private insurance was associated with a lower likelihood for referral (OR = 0.32, 95% CI [0.12–0.80]. Of those referred, 49 (76.6%) completed an appointment, and 47 had genetic testing. Women with Medicare were also less likely to complete an appointment. Race had no impact on referral or appointment completion.ConclusionsThere were no racial differences in GC referral or appointment completion in a clinic‐based sample of women with breast cancer. Further interventions are needed to promote increased referral and appointment completion for women with breast cancer who are eligible for GC.
Comprehensive evaluation of the test for 5′‐/3′‐end mRNA unbalanced expression as a screening tool for ALK and ROS1 fusions in lung cancer
Authors: Mitiushkina Natalia V. , Romanko Alexandr A. , Preobrazhenskaya Elena V. , Tiurin Vladislav I. , Ermachenkova Tatiana I. , Martianov Alexandr S. , Mulkidjan Rimma S. , Sokolova Tatiana N. , Kholmatov Maksim M. , Bizin Ilya V. , Ivantsov Alexandr O. , Yatsuk Olga S. , Zaitseva Olga A. , Iyevleva Aglaya G. , Kuligina Ekatherina Sh. , Imyanitov Evgeny N. 
Published: March 2022

AbstractBackgroundDespite the progress in the development of next‐generation sequencing (NGS), diagnostic PCR assays remain to be utilized in clinical routine due to their simplicity and low cost. Tests for 5′‐/3′‐end mRNA unbalanced expression can be used for variant‐independent detection of translocations, however, many technical aspects of this methodology require additional investigations.MethodsKnown ALK/ROS1 fusions and 5′‐/3′‐end unbalanced expression were analyzed in 2009 EGFR mutation‐negative non‐small cell lung cancer (NSCLC) samples with RT‐PCR tests, which were optimized for the use with FFPE‐derived RNA.ResultsVariant‐specific PCR tests for 4 common ALK and 15 common ROS1 translocations detected 115 (5.7%) and 44 (2.2%) rearrangements, respectively. Virtually all samples with common ALK fusions demonstrated some level of 5′/3′ mRNA ends unbalanced expression, and 8 additional NSCLCs with rare ALK fusions were further identified by PCR or NGS among 48 cases selected based on ALK expression measurements. Interestingly, NSCLCs with unbalanced 5′‐/3′‐end ALK expression but without identified ALK translocations had elevated frequency of RAS mutations (21/40, 53%) suggesting the role of RAS activation in the alternative splicing of ALK gene. In contrast to ALK, only a minority of ROS1 translocation‐positive cases demonstrated unbalanced gene expression, with both 5′‐ and 3′‐end mRNA expression being elevated in most of the samples with translocations. Surprisingly, high ROS1 expression level was also found to be characteristic for NSCLCs with activating mutations in other tyrosine kinases such as EGFR, ALK, or MET.ConclusionsComprehensive ALK analysis can be performed by the test for 5′‐/3′‐end unbalanced expression with minimal risk of missing an ALK rearrangement. In contrast, the use of the test for 5′‐/3′‐end unbalanced expression for the detection of ROS1 fusions is complicated; hence, the utilization of variant‐specific PCR assays for ROS1 testing is preferable.
Patient‐reported outcomes provide prognostic information for survival in patients with diffuse large B‐cell lymphoma: Analysis of 1239 patients from the GOYA study
Authors: Huang Huang , Datye Asim , Fan Ming , Knapp Andrea , Nielsen Tina , Bottos Alessia , Paulson Joseph N. , Trask Peter C. , Efficace Fabio 
Published: March 2022

AbstractPurposeWe investigated the prognostic value of pretreatment patient‐reported outcomes (PROs) in patients with diffuse large B‐cell lymphoma (DLBCL) receiving obinutuzumab/rituximab plus chemotherapy in the GOYA phase III study.MethodsPatients completed the European Organization for Research and Treatment of Cancer Quality of Life (EORTC QLQ‐C30) and the Functional assessment of chronic illness therapy‐Lymphoma (FACT–Lym) lymphoma subscale (LYMS) during the study. PRO scales with high prognostic value were identified through Cox regression analyses of overall survival (OS) and progression‐free survival (PFS). These scales were evaluated in terms of their additional prognostic value beyond the International Prognostic Index (IPI). A preliminary assessment was performed to evaluate whether the scales provided improved patient‐risk stratification beyond IPI.ResultsOne thousand two hundred and fifty‐nine patients with valid pretreatment PRO scales were included in the analyses, and complete pretreatment data were available for 1239/1414 patients (87.6%). Four PRO scales with high prognostic value were identified: FACT–Lym LYMS and EORTC QLQ‐C30 physical functioning, global health status/quality of life (QoL), and fatigue. All four scales retained significant prognostic value for OS and PFS after IPI adjustment (all p < 0.05). After adjusting for multiple clinical variables (IPI, cell of origin, BCL2 status, and total metabolic tumor volume), all four scales retained significant prognostic value (all p < 0.05) for OS. Only the EORTC QLQ‐C30 physical functioning scale was significant (p < 0.05) for PFS after adjustment for multiple clinical variables.ConclusionsIn this large population of patients with DLBCL, pretreatment PROs provided prognostic information for OS and PFS beyond the well‐established IPI.
Depression before and after diagnostic procedures among women with abnormal finding of Papanicolaou screening test
Authors: Ilic Irena , Babic Goran , Dimitrijevic Aleksandra , Grujicic Sandra Sipetic , Ilic Milena D. 
Published: March 2022

AbstractBackgroundSome studies did find significant differences in the level of depression of women while undergoing diagnostic evaluation of an abnormal Papanicolaou screening smear, but findings were not consistent. This study aimed to assess prevalence and correlates of depression in women with abnormal cervical screening results before and after diagnostic procedures.MethodsA cross‐sectional study was carried out during 2017 in a cohort of women with positive Papanicolaou screening test before and after diagnostic procedures (colposcopy/biopsy/endocervical curettage) at the university Clinical Centre Kragujevac, Serbia. Women completed a questionnaire about demographics, lifestyle, and other factors of interest. Also, questionnaire “Hospital Anxiety and Depression Scale” (HADS) was used immediately before and 2–4 weeks after the diagnostic procedures: a score of ≥8 on HADS‐D and HADS‐A subscales indicated depression and anxiety, respectively. Multivariate logistic regression was applied in the data analysis.ResultsThe study comprised 172 women, giving a response rate of 72.3%. The mean age of the participants was 47.8 ± 11.1 years (range 23–65). The frequency of depressive symptoms was significantly higher after diagnostic procedures (48.3%) than before diagnostic procedures (37.2%) (p = 0.038). Before diagnostic procedures, older age (OR = 1.60; 95% CI = 1.09–2.34; p = 0.017), and level of anxiety according to the HADS‐A subscale (OR = 1.61; 95% CI = 1.38–1.88; p < 0.001) were significant independent predictors of depression. After diagnostic procedures, significant independent predictors of depression were urban place of residence (OR = 0.12; 95% CI = 0.03–0.47; p = 0.002) and level of anxiety according to the HADS‐A subscale (OR = 1.85; 95% CI = 1.54–2.21; p < 0.001).ConclusionOur study showed that older age, rural residence, and anxiety play a role in shaping the risk of depression among women undergoing additional diagnostic procedures after receiving an abnormal Papanicolaou screening result.