bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variability of secondary metabolites from leaves of Ziziphus mauritiana obtained from different locations in Sumbawa, Indonesia
Authors: NAIRFANA IHLANA , NIKMATULLAH ALUH , SARJAN MUHAMMAD , TANDEANG ADNAN 
Published: November 2022

Abstract. Nairfana I, Nikmatullah A, Sarjan M, Tandeang A. 2022. Variability of secondary metabolites from leaves of Ziziphus mauritiana obtained from different locations in Sumbawa, Indonesia. Biodiversitas 23: 4948-4958. Bidara (Ziziphus mauritiana L.) is an evergreen shrub that grows in many parts of the world, and is an important plant in arid and semi-arid regions for the environment, food, and health. The plant has been used traditionally as medicine, and more recently, growing scientific evidence demonstrated the active compounds of bidara leaves. However, little is known about the plant's secondary metabolite profiles in different environments. This research aimed to characterize the secondary metabolites profile of bidara leaf obtained from the different habitats of semi-arid Sumbawa Island, Indonesia, and to evaluate their relationship based on cluster analysis of the secondary metabolite diversity. The leaf samples were collected from bidara plants obtained in 3 districts on Sumbawa Island: West Sumbawa (SB), Sumbawa (KS), and Dompu (DP) Districts, each from different ecosystems: lowland coastal/savanna, lowland near residential areas, and mid-highland areas of residential or forest-edge. The leaf samples were extracted by maceration with multilevel extraction using solvents with decreased polarity (h-hexane, ethyl acetate, ethanol 70%, methanol, and distilled water, respectively), followed by phytochemical screening for the presence of alkaloid, flavonoid, saponin, and phenolic. The results showed variation in the phytochemical components of bidara leaves obtained from a different environment, with secondary metabolites of DP leaves being more diverse than KS and other SB. All samples contained polar alkaloids and flavonoids, while the DP1 samples also contained non-polar flavonoids. The SB1 and DP samples also contained saponin. Interestingly, phenolic was only detected in the polar extracts of DP1 leaves. Cluster analysis based on the secondary metabolites profile distinguished the SB1 and DP1 bidara from others. Further study is required to understand if the variation is resulted from bidara adaptation to a different environment or also due to genetic variation of the bidara in Sumbawa Island.
Effect of Aspergillus niger extract on production of coumarins in cell suspension cultures of Angelica archangelica
Authors: NADIR DHUHA S. 
Published: November 2022

Abstract. Nadir DS. 2022. Effect of Aspergillus niger extract on production of coumarins in cell suspension cultures of Angelica archangelica. Biodiversitas 23: 5132-5138. Aspergillus niger is one of the most significant microbes employed in biotechnology and has been used for many years to make citric acid and extracellular enzymes. It is also utilized for waste treatment and biotransformation. In the last 20 years, A. niger has become a crucial transformational host to overexpress food enzymes. Different concentrations of A. niger extract were used to investigate their effects on the production of coumarin, both in the liquid medium and the cells, of batch cultures of Angelica archangelica L. Liquid Murashige and Skoog (MS) medium enriched with 2.0 mg.L-1 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0.5 mg.L-1 Kinetin (Kin), was used as growth medium for batch cultures of A. archangelica incubated for different incubation periods (7, 14 and 21 days). The results showed that 3.053 mg.g-1 was the highest coumarin concentration, obtained from the nutritional medium drawn from cultures treated with 2.0 mL.L-1 of A. niger after 21 days of incubation. However, 2.055 mg.g-1 was the maximum coumarin concentration reached in the harvested cells after 21 days of incubation when the cultures were treated with 2.0 mL.L-1 of A. niger extract. This study was aimed at performing extraction, identification, and estimation of coumarins from cell suspension cultures of A. archangelica and also investigating the effects of A. niger extract, at different concentrations, for various incubation periods on the production of coumarins. The concluded that A. niger extract affected coumarin accumulation, both in the medium and cells, from the cultures of A. archangelica. A dose of 2.0 mL.L-1 of A. niger extract as a biotic elicitor was found to have the best elicitor concentration, and that 21 days of incubation was the optimum period for the elicitor concentration to produce the highest coumarin content in batch cultures. Therefore, these results may contribute to increasing coumarin production by using different fungal extracts as biotic elicitors in cell suspension cultures.
Morphological characters variation of Indonesian accession Echinacea purpurea in response to gamma-ray irradiation
Authors: Cahyaningsih Agustina Putri , Etikawati‬ ‪Nita , Yunus Ahmad 
Published: November 2022

Abstract. Cahyaningsih AP, Etikawati N, Yunus A. 2022. Morphological characters variation of Indonesian accession Echinacea purpurea in response to gamma-ray irradiation. Biodiversitas 23: 5351-5359. Indonesia was one of the countries that introduced E. purpurea as a medicinal plant. Accessions of E. purpurea that have been successfully cultivated in Indonesia have narrow genetic diversity, lack accession variation, and have almost uniform tillers. This study was conducted to determine the effect of different doses of gamma-ray irradiation on morphological characteristics of E. purpurea accession B2P2TOOT. The experimental design was a Randomized Block Design with three replications, and six doses of gamma-ray irradiation (0, 15 Gy, 20 Gy, 25 Gy, 40 Gy, and 60 Gy) were used. The qualitative morphological data were presented descriptively; quantitative data were analyzed using ANOVA followed by a DMRT test at a 5% level with SPSS 16.0 application. The Dice similarity algorithm analyzed the similarity index, group analysis, and dendrogram construction using the UPGMA method with the NTSYS 2.02 application. Gamma-ray irradiation treatment increased the survival rate of E. purpurea plants grown in tropical lowlands. A dose of 15-60 Gy gamma irradiation did not affect the qualitative morphology of E. purpurea roots, stems, and leaves. Irradiation at doses of 40 Gy and 60 Gy resulted in flowers with more variation in color, overall flower shape, and arrangement of ray floret. Gamma irradiation significantly affected plant height, leaf length, leaf area, flower angle, and the first day of flowering. The 40 and 60 Gy doses resulted in longer leaves with wider leaf surfaces. The dendrogram revealed that E. purpurea irradiation resulted in two main groups, with doses of 40 and 60 Gy forming their groups and increasing morphological variation by 30% compared to controls.
biblioteques públiques a Catalunya (2020-2021)
Authors: Morlans Giné Gemma , Sánchez Contreras Maria del Mar 
Published: November 2022

En aquest capítol es descriuen i s’analitzen els esdeveniments i les accions més rellevants duts a terme en l’àmbit de les biblioteques públiques de Catalunya durant el bienni 2020-2021.
Sens dubte, el bienni ha estat marcat per la pandèmia de la COVID-19 i hem vist com les biblioteques han hagut de fer front al tancament dels equipaments i a adaptar els serveis i activitats a la nova situació. Després d’estabilitzar el sistema, es van impulsar diverses accions per pal·liar la manca d’obertura física, especialment en format virtual, però també per a persones amb risc de vulnerabilitat. Amb la reobertura, a poc a poc, els serveis es van recuperant, camí d’assolir els nivells anteriors a la pandèmia. Tot i la situació, l’activitat professional en jornades, congressos i esdeveniments no ha desaparegut i s’ha adaptat a formats virtuals o híbrids.
En aquests dos anys també hi ha hagut canvis tècnics en processos com ara catalogació i canvis de programari, i s’ha materialitzat la interfície de petició de documents en préstec interbibliotecari de totes les biblioteques del Sistema de Lectura Pública de Catalunya.
En definitiva, l’objectiu del capítol és mostrar una visió de conjunt de l’activitat de les biblioteques públiques catalanes per reflectir el moment actual i veure les tendències de futur.
Impairment in quantitative microvascular function in non-ischemic cardiomyopathy as demonstrated using cardiovascular magnetic resonance
Authors: Slivnick Jeremy A. , Zareba Karolina M. , Truong Vien T. , Liu Ellen , Barnes Alexis , Mazur Wojciech , Binkley Philip 
Published: November 2022

Background
Microvascular dysfunction (MVD) is present in various cardiovascular diseases and portends worse outcomes. We assessed the prevalence of MVD in patients with non-ischemic cardiomyopathy (NICM) as compared to subjects with preserved ejection fraction (EF) using stress cardiovascular magnetic resonance (CMR).

Methods
We retrospectively studied consecutive patients with NICM and 58 subjects with preserved left ventricular (LV) EF who underwent stress CMR between 2011–2016. MVD was defined visually as presence of a subendocardial perfusion defect and semiquantitatively by myocardial perfusion reserve index (MPRI<1.51). MPRI was compared between groups using univariate analysis and multivariable linear regression.

Results
In total, 41 patients with NICM (mean age 51 ± 14, 59% male) and 58 subjects with preserved LVEF (mean age 51 ± 13, 31% male) were identified. In the NICM group, MVD was present in 23 (56%) and 11 (27%) by semiquantitative and visual evaluation respectively. Compared to those with preserved LVEF, NICM patients had lower rest slope (3.9 vs 4.9, p = 0.05) and stress perfusion slope (8.8 vs 11.7, p<0.001), and MPRI (1.41 vs 1.74, p = 0.02). MPRI remained associated with NICM after controlling for age, gender, hypertension, ethnicity, diabetes, and late gadolinium enhancement (log MPR, β coefficient = -0.19, p = 0.007).

Conclusions
MVD—as assessed using CMR—is highly prevalent in NICM as compared to subjects with preserved LVEF even after controlling for covariates. Semiquantitative is able to detect a greater number of incidences of MVD compared to visual methods alone. Further studies are needed to determine whether treatment of MVD is beneficial in NICM.
Prevalence and clinical characteristics of headache among medical students of Nepal: A cross-sectional study
Authors: Bhattarai Ayush Mohan , Gurung Shekhar , Pathak Bishnu Deep , Karki Saurab , Adhikari Ayush , Tandon Om Prakash , Poudel Sabin , Yadav Deepak , Pant Chiranjibi , Dhakal Bishal 
Published: November 2022

Background
Headache is a common neurological disorder, with a global prevalence of around 50%. It may affect people of any age, gender, education, socioeconomic status and occupation. Tension headache, migraine headache and cluster headache are commonly encountered headache types. The prevalence of headache problems is higher among medical students. This could potentially affect their academic performance and quality of life. The objective of this study is to find out the prevalence of headaches and their clinical characteristics among students of a medical college.

Materials and methods
An online, single-centre, cross-sectional study was conducted among undergraduate medical students in Nepal. Stratified sampling followed by a simple random sampling technique was adopted depending upon the academic years of students. For data collection, pre-tested semi-structured questionnaire was used. The data entry and analysis were done by using Statistical Package for the Social Sciences (IBM-SPSS), version-23. The prevalence of headache and its subtypes were calculated. All the clinical characteristics associated with headaches were also studied.

Results
The prevalence of headache disorder was 65 (26.86%), with tension headache (69.23%) being the commonest one. It was highest among fourth-year students (37.84%) followed by first-year students (33.33%). Anxiety/stress (75.40%) was the most common precipitating factor. This problem stopped most of the students (63.09%) from doing daily activities. More than half of them (53.85%) practised self-medication in case of non-resolution of pain, and non-steroidal anti-inflammatory drugs (NSAIDs) were frequently used.

Conclusion
Headache was fairly prevalent among medical students. Anxiety/stress in medical life has led to headaches in the majority of students. The headache disturbed their daily activities and promoted self-medication practice. So, this problem should be properly looked into and addressed in time by the concerned authority.
Dissociative experiences among Lebanese university students: Association with mental health issues, the economic crisis, the COVID-19 pandemic, and the Beirut port explosion
Authors: Mhanna Mariam , El Zouki Christian-Joseph , Chahine Abdallah , Obeid Sahar , Hallit Souheil 
Published: November 2022

BackgroundDissociative experiences are psychological manifestations characterized by a loss of connection and continuity between thoughts, emotions, environment, behavior, and identity. Lebanon has been facing indescribable events in the last few years, including the COVID-19 pandemic, the Beirut explosion, a crushing economic crisis with the highest inflation rate the country has known in over three decades. The aim of this study was to evaluate the correlation between dissociative experiences and post-traumatic stress symptoms from the economic crisis, the Beirut blast, the COVID-19 pandemic, and other mental health issues in a sample of Lebanese university students.MethodsThis cross-sectional study enrolled 419 active university students (18–35 years) from all over Lebanon (May and August 2021). The respondents received the online soft copy of a survey by a snowball sampling technique through social media and messaging apps. The questionnaire included sociodemographic data, the Dissociative Experience Scale (DES-II), the PTSD Checklist Specific Version (PCL-S), the Financial Wellbeing Scale, the Beirut Distress Scale, the Lebanese Anxiety Scale, the Patient Health Questionnaire.ResultsThe two-factor model of the DES fitted best according to CFI, RMSEA and χ2/df values, but modestly according to TLI. The two factors were absorption and amnesia/depersonalization. Higher stress (Beta = 0.95) and more PTSD from the Beirut blast (Beta = 0.29) and from the economic crisis (Beta = 0.23) were significantly associated with more absorption. A personal history of depression (Beta = 6.03), higher stress (Beta = 0.36) and more PTSD from the Beirut blast (Beta = 0.27) and from the COVID-19 pandemic (Beta = 0.16) were significantly associated with more amnesia/depersonalization.ConclusionSignificant rates of dissociative experiences and their sub-manifestations (amnesia/depersonalization and absorption) were found among Lebanese university students, with remarkable co-occurrence of a traumatic/stressful pattern, whether on an individual (history of PTSD) or a collective level (Post-traumatic manifestations from Beirut blast, COVID-19 pandemic and/or economic crisis), or whether correlated to an acute single event or to certain chronic stressors, or even to a personal history of depression. Such findings must raise the attention to serious mental and psychosocial alteration in the Lebanese national identity.
Multidisciplinary team healthcare professionals’ perceptions of current and optimal acute rehabilitation, a hip fracture example A UK qualitative interview study informed by the Theoretical Domains Framework
Authors: Guerra Stefanny , Lambe Kate , Manolova Gergana , Sadler Euan , Sheehan Katie J. 
Published: November 2022

Objective
To understand multidisciplinary team healthcare professionals’ perceptions of current and optimal provision of acute rehabilitation, perceived facilitators and barriers to implementation, and their implications for patient recovery, using hip fracture as an example.

Methods
A qualitative design was adopted using semi-structured telephone interviews with 20 members of the acute multidisciplinary healthcare team (occupational therapists, physiotherapists, physicians, nurses) working on orthopaedic wards at 15 different hospitals across the UK. Interviews were audio-recorded, transcribed verbatim, anonymised, and then thematically analysed drawing on the Theoretical Domains Framework to enhance our understanding of the findings.

Results
We identified four themes: conceptualising a model of rehabilitative practice, which reflected the perceived variability of rehabilitation models, along with facilitators and common patient and organisational barriers for optimal rehabilitation; competing professional and organisational goals, which highlighted the reported incompatibility between organisational goals and person-centred care shaping rehabilitation practices, particularly for more vulnerable patients; engaging teams in collaborative practice, which related to the expressed need to work well with all members of the multidisciplinary team to achieve the same person-centred goals and share rehabilitation practices; and engaging patients and their carers, highlighting the importance of their involvement to achieve a holistic and collaborative approach to rehabilitation in the acute setting. Barriers and facilitators within themes were underpinned by the lack or presence of adequate ways of communicating with patients, carers, and multidisciplinary team members; resources (e.g. equipment, staffing, group classes), and support from people in leadership positions such as management and senior staff.

Conclusions
Cornerstones of optimal acute rehabilitation are effective communication and collaborative practices between the multidisciplinary team, patients and carers. Supportive management and leadership are central to optimise these processes. Organisational constraints are the most commonly perceived barrier to delivering effective rehabilitation in hospital settings, which exacerbate silo working and limited patient engagement.
Study of phylogenetic relationships between wild and cultivated oat species (Avena L.)
Authors: Gnutikov A. A. , Nosov N. N. , Loskutov I. G. , Blinova E. V. , Rodionov A. V. 
Published: November 2022

We performed next generation sequencing (NGS) of wild and cultivated oat species (Avena L.) ITS1 sequence and the beginning of the gene 5.8S rRNA on the Illumina platform. For the first time, the NGS method was used to carry out a phylogenetic analysis of the domestication pathways of three cultivated oat species: tetraploid A. abyssinica (AB), hexaploid A. sativa and A. byzantina (ACD). We found that the A. abyssinica genome with the most massive number of reads is the A-genome, while in A. sativa, it is the D-genome. Also, we studied the putative ways of obtaining polyploid genomes from diploid species. According to NGS sequencing data, it was revealed that the most widespread A. sativa ribotype is inherited from A. ludoviciana, A. byzantina has two unique families of ribotypes, and A. abyssinica probably comes from the wild A. vaviloviana (A-genome was obtained by this species from the diploid A. atlantica (As-genome)). Putative ancestor of A. abyssinica, tetraploid A. agadiriana, forms unique families of ribotypes, to which the most widespread ribotype belongs. It most likely was not a direct ancestor of A. abyssinica. C-genome species A. clauda turned out to be related to the D-genome of A. sativa; it could also be involved in the formation of the hexaploid A. ludoviciana.
Study of ribotypes of experimentally obtained hybrids of polyploid species of the genus Avena L.
Authors: Gnutikov A. A. , Nosov N. N. , Loskutov I. G. , Blinova E. V. , Rodionov A. V. 
Published: November 2022

For the first time, using next generation sequencing (NGS) on the Illumina platform of the ITS1 sequence and the beginning of the 5.8S rRNA gene, the current state of genomic compositions of complex cultivated oat hybrids based on the Avena macrostachya was studied. It was shown that A. sativa х A. macrostachya hybrid used for further hybridization with other oat species actually acts as a “pure” A. sativa in the next generations of hybridization. The most represented ribotypes (abundant by the number of marker sequences reads) are inherited from the hexaploid A. sativa (ACD genome). Other parental taxa, such as hexaploids A. byzantina, A. sterilis (ACD-genome), tetraploids A. magna, A. murphyi (AC-genome), as a rule, form the second most abundant ribotype in hybrids. Also, this ribotype includes the minor components of A. sativa rDNA (probably left over from common ancestors). We assume that this is due to the harsh consequences of the reorganization of genomes in interspecific hybrids from crossing cultivated oat with wild ones, as a result of which the number of sequences of these species in the genomic set of hybrids naturally gradually decreases. It especially manifests in the artificial hybridization of hexaploids of the A. sativa group (A. sativa and A. fatua, ACD-genome) and A. macrostachya (homotetraploid CmCm). In such hybrids, according to our data, the loss of entire chromosomes of A. macrostachya sometimes occurs, due to which we do not see rDNA sequences of this species at all.
Morphological and anatomical structure of two species of the genus Alchemilla L. (Rosaceae Juss.) in the Alpine belt of the northwestern Caucasus
Authors: Lyubeznova N. V. 
Published: November 2022

Two species of the genus Alchemilla L. growing in the alpine zone of the Northwestern Caucasus are considered. A. sericea Willd. occupies rock crevices, A. caucasica Buser grows in alpine lichen tundra. A. sericea, in comparison with A. caucasica, has more compact sizes, a reduced leaf series, rare branching, and later formation of adventitious roots, which secondarily thicken only one season. Its main root is secondary thickens and preserved for a long time. When dying, it is replaced by a lateral one, in which an additional cambium is formed for secondary thickening. A. caucasica forms more branches and adventitious roots to feed and take over new territory. It has the system of epigeogenic rhizomes with a supply of nutrients lying in the upper soil layer. In anatomy, the general plan of the structure of organs is preserved, with the exception of the structure of the main root. A. caucasica is characterized by the accumulation of orange or black contents in the endodermis, phloem, in the cells layer under the epidermis, to protect against the adverse effects of ultraviolet light.
New growth locations of Artemisia sieversiana Willd. in the Western Pamir
Authors: Miravalova G. Sh. , Kurbonbekova Sh. Sh. 
Published: November 2022

The article presents data on the place of growth of Artemisia sieversiana Willd. in the Western Pamirs, previously unpublished. This medicinal plant is well known to the locals of the area and is widely used in folk medicine. Some literature shows a limited distribution of this species, however, the plant has a wider distribution in the Western Pamirs. During our studies on the study of the habitat of A. sieversiana in the Western Pamirs, 11 new localities of this species were found, 6 of which belong to the Shugnan and 5 to the Ishkashim regions. During expeditionary research, we found that A. sieversiana is widespread in many villages of Porshinev. It can be found in the villages of Barchid, Midenshor, Tishor, Ji-ruj, etc. In the Gund valley of the Shugnan region, it can be found in the villages of Charsem and Sardem. Also in the Ish-kashim region, the plant grows in the villages of Kozideh, Yakhshvol, Boypar, Ratm and Yamchun. which refers to the new localities of this species in the Western Pamirs. In the Roshtkala district of the A. sieversiana, it grows from 2100 to 3840 m above sea level in the river valley, in gardens and near residential areas.