bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expansion of the EZSTATSG1 Microsoft Excel Tool for ANSI/ASB Standard 036 Method Validation by the Addition of Weighted Quadratic External Calibration Models Utilizing Five-, Six- or Seven-Point Curves
Authors: Sofalvi Szabolcs , Schueler Harold E , Abonamah Jocelyn V 
Published: June 2022

Abstract
The first generation of this Microsoft (MS) Excel (Redmond, WA, USA) tool for method validation, EZSTATSG1, was designed for methods utilizing only linear calibration curves requiring seven calibration levels, and quadratic calibration models were not supported. This significantly improved version, EZSTATSG2, includes all of the features of the original template such as weighted linear calibration models, bias and precision data, dilution integrity and ion suppression. New features in this version include flexible five-, six- or seven-point calibration curves; six weighted quadratic calibration models; standardized residuals by use of frequency plots overlaid with the normal distribution function along with five-number summary data and processed sample stability. The implementation of Visual Basic for Applications in Excel UserForms prevents accidental alteration of existing formulas and also ensures that pertinent cells are relocked every time a file is reopened. The quadratic models feature the 95% confidence intervals for checking the significance of the second-order term and are fully characterized by providing the equations for the axis of symmetry, directrix and coordinates for vertex and focus. Example data of α-hydroxymidazolam demonstrate that the quadratic calibration curves fit the data more adequately than the linear models for this method. This second-generation tool summarizes all of the validation parameters of a method for both linear and quadratic calibration models. Models with the lower average sum of relative errors and higher R2 values are color-coded green, indicating the likelihood of a better fitting model. Like the EZSTATSG1, the redesigned EZSTATSG2.xltm MS Excel self-actuating validation tool and a completed PDF example are available to the scientific community for download as supplementary data. Updates to the template can be found at www.EZSTATS4validation.com.
Cannabinoid Stability in Postmortem Brain Samples Stored at Different Temperatures
Authors: Santunione Anna L , Palazzoli Federica , Verri Patrizia , Vandelli Daniele , Chiapelli Francesca , Silingardi Enrico 
Published: June 2022

AbstractDrug stability is an important concern of forensic toxicological testing, particularly postmortem (PM) samples that may be stored for an extensive period of time before analysis. In PM toxicology, the complex assessment of analyte stability in biological matrices can profoundly impact the interpretation of toxicological results and the outcome of forensic casework. The aim of this work is to assess the stability of ∆9-tetrahydrocannabinol (THC), cannabidiol, 11-hydroxy-THC (11-OH-THC), 11-nor-9-carboxy-∆9-THC (THCCOOH) and 11-nor-∆9-THC-9-carboxylic acid glucuronide (THCCOOH-glucuronide) in brain stored at three different temperatures (4°C, −20°C and −70°C) up to over 12 months (390 days) in order to establish the best storage condition for preventing potential drug degradation during the storage period. Brain is suitable and useful for xenobiotic concentrations and is a valuable specimen in the interpretation of PM toxicological results. In our study, pooled brain specimens were spiked at low and high concentrations to evaluate the change in concentration over time. Stable compounds were quantified within ±20% of the target concentration (the mean concentration resulting from the initial analysis). According to stability criteria, our preliminary findings revealed that all the cannabinoids studied are stable in frozen brain samples (−20°C and −70°C) for over 12 months: all the analytes’ concentrations remained unaffected during storage over time, with the analytical variation staying within ±20%. On the contrary, under refrigeration conditions (4°C), 11-OH-THC, THCCOOH and THCCOOH-glucuronide were instable. Authentic brain samples, collected from eight cases during the autopsy, were analyzed, and the stability was evaluated. This study provided new data on cannabinoid stability in brain. The stability of the brain samples, both in spiked samples and in authentic caseworks, highlights the importance of the brain as a valid testing matrix when retesting is required after a long period of time or when laboratories are faced with backlog.
Getting and Feeling Safer, Part 2
Authors: Schielke Hugo J. , Brand Bethany L. , Lanius Ruth A. 
Published: June 2022

Abstract
This module builds on all you’ve already done as part of this program and focuses on learning more ways to help yourself recognize, interrupt, and reduce things that get in the way of getting and feeling safer. Toward these ends, this module will help you learn about your “window of tolerance” and how to calm your alarm system. You will practice getting and feeling safer by staying within and slowly expanding your window of tolerance. It will also help you practice having safer experiences, doing things that help calm your alarm system, and reducing behaviors that get in the way of getting and feeling safer. You will also learn about reducing your exposure to unhealthy and retraumatizing experiences and ways to help yourself when your risk of doing unhealthy or unsafe things is increasing.
The Self-Healing Mind
Authors: McVeigh Brian J. 
Published: June 2022

AbstractEvolutionary psychology/psychiatry teaches us about why some mental illnesses developed. However, Brian J. McVeigh argues that much more recent changes in mentality hold lessons about improving our mental well-being. Indeed, by around 1000 bce, population expansion and social complexity had forced people to learn conscious interiority, a package of capabilities that culturally upgraded mentality. The functions/features of conscious interiority (FOCI) are instances of adaptive meta-framing: abstracting, metaphorizing, reframing, and transcending one’s circumstances. Adopting a common factors and positive psychology perspective, McVeigh enumerates FOCI—“active ingredients”—of the self-healing mind: mental space (introspectable stage for manipulating mental images); introception (employing semi-hallucinatory quasi-perceptions to “see” different perspectives); self-observing and observed (increasing role/perspective-taking); self-narratization (intensifying retrospection/prospection capabilities); excerption (editing mental contents for higher-order conceptualization); consilience (fitting conceptions together more effectively to bolster abstraction); concentration (peripheralizing unrelated mental material); suppression (deleting distracting and distressing thoughts); self-authorization (a sense of who or what one’s legitimizes one’s decision and behavior); self-autonomy (bolstering self-direction and self-confidence); self-individuation (highlighting personal strengths); self-reflexivity (cultivating insight, self-objectivity, and self-corrective abilities). FOCI underlie the effectiveness of psychotherapeutic techniques. Though the psyche’s recuperative properties correct distorted cognition and provide remarkable adaptive abilities, FOCI sometimes spiral out of control, resulting in runaway consciousness and certain mental disorders. Also addressed, then, is how snowballing FOCI become maladaptive processes in need of restraint. The benefits of temporarily suspending FOCI (hypnosis) and regulating them (meditation) are also explored. This work will appeal to practitioners, researchers, and anyone interested in how therapeutically directed consciousness repairs the mind.
PUMILIO competes with AUF1 to control DICER1 RNA levels and miRNA processing
Authors: Rajasekaran Swetha , Khan Eshan , Ching Samuel R , Khan Misbah , Siddiqui Jalal K , Gradia Daniela F , Lin Chenyu , Bouley Stephanie J , Mercadante Dayna L , Manning Amity L , Gerber André P , Walker James A , Miles Wayne O 
Published: June 2022

Abstract
DICER1 syndrome is a cancer pre-disposition disorder caused by mutations that disrupt the function of DICER1 in miRNA processing. Studying the molecular, cellular and oncogenic effects of these mutations can reveal novel mechanisms that control cell homeostasis and tumor biology. Here, we conduct the first analysis of pathogenic DICER1 syndrome allele from the DICER1 3′UTR. We find that the DICER1 syndrome allele, rs1252940486, abolishes interaction with the PUMILIO RNA binding protein with the DICER1 3′UTR, resulting in the degradation of the DICER1 mRNA by AUF1. This single mutational event leads to diminished DICER1 mRNA and protein levels, and widespread reprogramming of miRNA networks. The in-depth characterization of the rs1252940486 DICER1 allele, reveals important post-transcriptional regulatory events that control DICER1 levels.
Host cell RecA activates a mobile element-encoded mutagenic DNA polymerase
Authors: Ojha Debika , Jaszczur Malgorzata M , Sikand Adhirath , McDonald John P , Robinson Andrew , van Oijen Antoine M , Mak Chi H , Pinaud Fabien , Cox Michael M , Woodgate Roger , Goodman Myron F 
Published: June 2022

AbstractHomologs of the mutagenic Escherichia coli DNA polymerase V (pol V) are encoded by numerous pathogens and mobile elements. We have used Rum pol (RumA′2B), from the integrative conjugative element (ICE), R391, as a model mobile element-encoded polymerase (MEPol). The highly mutagenic Rum pol is transferred horizontally into a variety of recipient cells, including many pathogens. Moving between species, it is unclear if Rum pol can function on its own or requires activation by host factors. Here, we show that Rum pol biochemical activity requires the formation of a physical mutasomal complex, Rum Mut, containing RumA′2B-RecA-ATP, with RecA being donated by each recipient bacteria. For R391, Rum Mut specific activities in vitro and mutagenesis rates in vivo depend on the phylogenetic distance of host-cell RecA from E. coli RecA. Rum pol is a highly conserved and effective mobile catalyst of rapid evolution, with the potential to generate a broad mutational landscape that could serve to ensure bacterial adaptation in antibiotic-rich environments leading to the establishment of antibiotic resistance.
G-quadruplex structure of the C. elegans telomeric repeat: a two tetrads basket type conformation stabilized by a non-canonical C–T base-pair
Authors: Marquevielle Julien , De Rache Aurore , Vialet Brune , Morvan Estelle , Mergny Jean-Louis , Amrane Samir 
Published: June 2022

Abstract
The Caenorhabditis elegans model has greatly contributed to the understanding of the role of G-quadruplexes in genomic instability. The GGCTTA repeats of the C. elegans telomeres resemble the GGGTTA repeats of the human telomeres. However, the comparison of telomeric sequences (Homo sapiens, Tetrahymena, Oxytricha, Bombyx mori and Giardia) revealed that small changes in these repeats can drastically change the topology of the folded G-quadruplex. In the present work we determined the structure adopted by the C. elegans telomeric sequence d[GG(CTTAGG)3]. The investigated C. elegans telomeric sequence is shown to fold into an intramolecular two G-tetrads basket type G-quadruplex structure that includes a C–T base pair in the diagonal loop. This work sheds light on the telomeric structure of the widely used C. elegans animal model.
H3K36me2 methyltransferase NSD2 orchestrates epigenetic reprogramming during spermatogenesis
Authors: Li Zhiming , Zhang Xinzong , Xie Shiming , Liu Xingping , Fei Caifeng , Huang Xunbin , Tang Yunge , Zhou Li-quan 
Published: June 2022

Abstract
Spermatogenesis is precisely controlled by sophisticated gene expression programs and is driven by epigenetic reprogramming, including histone modification alterations and histone-to-protamine transition. Nuclear receptor binding SET domain protein 2 (Nsd2) is the predominant histone methyltransferase catalyzing H3K36me2 and its role in male germ cell development remains elusive. Here, we report that NSD2 protein is abundant in spermatogenic cells. Conditional loss of Nsd2 in postnatal germ cells impaired fertility owing to apoptosis of spermatocytes and aberrant spermiogenesis. Nsd2 deficiency results in dysregulation of thousands of genes and remarkable reduction of both H3K36me2 and H3K36me3 in spermatogenic cells, with H3K36me2 occupancy correlating positively with expression of germline genes. Nsd2 deficiency leads to H4K16ac elevation in spermatogenic cells, probably through interaction between NSD2 and PSMA8, which regulates acetylated histone degradation. We further reveal that Nsd2 deficiency impairs EP300-induced H4K5/8ac, recognized by BRDT to mediate the eviction of histones. Accordingly, histones are largely retained in Nsd2-deficient spermatozoa. In addition, Nsd2 deficiency enhances expression of protamine genes, leading to increased protamine proteins in Nsd2-deficient spermatozoa. Our findings thus reveal a previously unappreciated role of the Nsd2-dependent chromatin remodeling during spermatogenesis and provide clues to the molecular mechanisms in epigenetic abnormalities impacting male reproductive health.
The Normativity of Law: Has the Dispositional Model Solved our Problem?
Authors: Vassiliou Andreas 
Published: June 2022

Abstract
In Legal Directives and Practical Reasons, Noam Gur has presented a novel account, called the dispositional model, to explain how law bears on our normative practical reasons. Gur holds that his model is superior to the current models, namely the standard weighing model and Joseph Raz’s exclusionary model. Although his work provides useful insights into the practical impact of law, I argue that: (i) his challenge against the exclusionary model is valid only insofar as one accepts Raz’s normal justification thesis and dependence thesis; (ii) his argument against the weighing model misses its target, because it attacks the model as a decision-making method, not as an account of practical reason; and (iii) his dispositional model solely constitutes a decision-making strategy and does not offer a third alternative answer to the question of how law affects our normative practical reasons. Hence, the dispositional model is not a competitor to the weighing and the exclusionary model, and the problem of accounting for the normativity of law remains.
A myosin XI adaptor, TAPE, is essential for pollen tube elongation in rice
Authors: Hong Woo-Jong , Kim Eui-Jung , Yoon Jinmi , Silva Jeniffer , Moon Sunok , Min Cheol Woo , Cho Lae-Hyeon , Kim Sun Tae , Park Soon Ki , Kim Yu-Jin , Jung Ki-Hong 
Published: June 2022

Abstract
Pollen tube (PT) elongation is important for double fertilization in angiosperms and affects the seed-setting rate and, therefore, crop productivity. Compared to Arabidopsis (Arabidopsis thaliana L.), information on PT elongation in rice (Oryza sativa L.) is limited by the difficulty in obtaining homozygous mutants. In a screen of T-DNA insertional mutants, we identified a mutant in the Tethering protein of actomyosin transport in pollen tube elongation (TAPE) gene with an unusual segregation ratio by genotyping analysis. A CRISPR/Cas9 knockout mutant of TAPE that produced a short PT was sterile, and TAPE was expressed specifically in pollen grains. TAPE is a homolog of a myosin XI adaptor in Arabidopsis with three tetratricopeptide repeat and Phox and Bem1 protein domains. TAPE showed latrunculin B-sensitive, actin-dependent localization to the endoplasmic reticulum. Yeast two-hybrid screening and transcriptome analysis revealed that TAPE interacted with pollen-specific LIM protein 2b and elongation factor 1-alpha. Loss of TAPE affected transcription of 1,259 genes, especially genes related to cell organization, which were downregulated. In summary, TAPE encodes a myosin XI adaptor essential for rice PT elongation.
Dance for Chronic Pain Conditions: A Systematic Review
Authors: Hickman Benjamin , Pourkazemi Fereshteh , Pebdani Roxanna N , Hiller Claire E , Fong Yan Alycia 
Published: June 2022

Abstract

Objectives
Globally, 20–25% of people will experience chronic pain in their lifetimes. Dance is a physical activity with psychosocial benefits that might positively impact pain. This review aimed to investigate the effect of dance interventions on the experience of pain by quantitative measures and qualitative themes.

Methods
Seven major databases were searched from inception to January 2021. Two independent reviewers screened articles at each stage. Qualitative and quantitative studies were included if the dance interventions lasted more than 6 weeks, participants reported pain of duration longer than 3 months, and pain was an outcome of the study. All articles were critically appraised with appropriate Joanna Briggs Institute tools, and data were collated through the use of results-based convergent synthesis.

Results
From 23,628 articles, 34 full papers were included, with a total of 1,254 participants (75.2% female). Studies predominantly investigated individuals with fibromyalgia (26%) and generalized chronic pain (14%), with aerobic dance (20.7%) and Biodanza (20.7%) being the most common dance genres investigated. Overall, 74% of studies noted either reduced pain through quantitative pain measures or qualitative themes of improved pain experience (88% for chronic primary pain and 80% for chronic secondary musculoskeletal pain).

Discussion
There were positive effects of dance on chronic primary and secondary musculoskeletal pain across diverse populations. A variety of study designs and interventions noted improved pain measures and themes around pain coping and acceptance, with all dance therapies showing improvements, particularly when performed for 60–150 minutes’ duration weekly. Dance should be considered as an effective adjunct in the management of chronic pain.
Prognostic Factors for Quality of Life After Interdisciplinary Pain Rehabilitation in Patients with Chronic Pain—A Systematic Review
Authors: Liechti Seraina , Tseli Elena , Taeymans Jan , Grooten Wilhelmus 
Published: June 2022

Abstract

Background
Health-related quality of life (hrQoL) is a core outcome in evaluating interdisciplinary pain rehabilitation (IPR). This systematic review aimed to identify prognostic factors for hrQoL at least six months after IPR in chronic pain patients.

Methods
A systematic search was conducted in MEDLINE, PsycINFO, EMBASE, CINAHL, Web of Science and Cochrane CENTRAL until September 2020. Included were prognostic studies on the outcome hrQoL in adults aged 18 to 67 years with chronic pain (excluding malignancies, systemic-, inflammatory or degenerative joint diseases) who had undergone IPR. Studies were assessed with The Quality in Prognostic Studies-tool. Potential prognostic factors at baseline for the domains pain, psychological and physical functioning were qualitatively synthesized for hrQoL. Grading of Recommendations Assessment, Development and Evaluation was used to evaluate the level of evidence.

Results
Fourteen studies on 6,668 participants (mean age 37.4–52.8 y), with musculoskeletal pain/fibromyalgia and a pain duration ranging between 13.1 and 177.4 months were considered eligible. With a very low certainty of evidence, pain intensity, emotional distress, and physical functioning at baseline were inconsistent for prediction of hrQoL and pain duration was not predictive. With low certainty of evidence, fewer pain sites, lower levels of negative cognitive behavioral factors, and higher levels of positive cognitive behavioral factors predicted a better outcome.

Conclusions
The overall certainty of evidence was low to very low, making it difficult to reach definitive conclusions at present. Future studies with a predefined core set of predictors investigating hrQoL in patients with chronic pain after IPR are needed.
Natural Language Processing and Psychosis: On the Need for Comprehensive Psychometric Evaluation
Authors: Cohen Alex S , Rodriguez Zachary , Warren Kiara K , Cowan Tovah , Masucci Michael D , Edvard Granrud Ole , Holmlund Terje B , Chandler Chelsea , Foltz Peter W , Strauss Gregory P 
Published: June 2022

Abstract

Background and Hypothesis
Despite decades of “proof of concept” findings supporting the use of Natural Language Processing (NLP) in psychosis research, clinical implementation has been slow. One obstacle reflects the lack of comprehensive psychometric evaluation of these measures. There is overwhelming evidence that criterion and content validity can be achieved for many purposes, particularly using machine learning procedures. However, there has been very little evaluation of test-retest reliability, divergent validity (sufficient to address concerns of a “generalized deficit”), and potential biases from demographics and other individual differences.

Study Design
This article highlights these concerns in development of an NLP measure for tracking clinically rated paranoia from video “selfies” recorded from smartphone devices. Patients with schizophrenia or bipolar disorder were recruited and tracked over a week-long epoch. A small NLP-based feature set from 499 language samples were modeled on clinically rated paranoia using regularized regression.

Study Results
While test–retest reliability was high, criterion, and convergent/divergent validity were only achieved when considering moderating variables, notably whether a patient was away from home, around strangers, or alone at the time of the recording. Moreover, there were systematic racial and sex biases in the model, in part, reflecting whether patients submitted videos when they were away from home, around strangers, or alone.

Conclusions
Advancing NLP measures for psychosis will require deliberate consideration of test-retest reliability, divergent validity, systematic biases and the potential role of moderators. In our example, a comprehensive psychometric evaluation revealed clear strengths and weaknesses that can be systematically addressed in future research.
Continuous Renal Replacement Therapy with Regional Citrate Anticoagulation in Children with Liver Dysfunction/Failure
Authors: Botan Edin , Durak Ayşen , Gün Emrah , Gurbanov Anar , Balaban Burak , Kahveci Fevzi , Özen Hasan , Uçmak Hacer , Aycan Fulden , Kendirli Tanıl 
Published: June 2022

Abstract
Regional citrate anticoagulation (RCA) is an option but citrate accumulation is risk and it is a giving up cause for this situation. This retrospective study was conducted in the pediatric intensive care unit (PICU) between May 2019 and April 2021. We investigated 47 patients with liver failure (LF) in our PICU, and RCA during continuous renal replacement therapy (CRRT) was applied to 10 (21.3%) of them. Half of them were male (n: 5/10), their mean age was 104.7 ± 66.20 months. Nine of them needed vasoactive support during follow-up. The most common indication for CRRT was hepatorenal syndrome (40%). There was no significant difference between liver transaminases and liver function tests before and after CRRT (p &gt; 0.05). In terms of citrate toxicity of the patients, there was no significant difference between total calcium/ionized calcium, lactate level, pH and bicarbonate values before and after CRRT (p &gt; 0.05). The mean total CRRT time was 110.2 ± 118.2 h, and the mean circuit lifespan was 43.8 ± 48.7 h; the mean number of circuits was 2.7 ± 2.4. Total Ca/ionized Ca &gt;2.5 was a clinically relevant endpoint, but no patient interrupted dialysis for this cause. There was no complication about RCA. This study did not observe any adverse effects on acid–base status, transaminases, an increase in bilirubin during RCA–CRRT treatment in pediatric patients with LF. Total calcium/ionized calcium ratio, serum lactate level and prothrombin time level should be closely monitored daily in terms of citrate accumulation in this patient group.