bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuropsychological, behavioral, and quality‐of‐life outcomes in children and adolescents with sickle cell disease treated with nonmyeloablative matched sibling donor hematopoietic cell transplantation: A case series
Authors: Fay‐McClymont Taryn B. , Monagel Dania A. , Singh Gurpreet , Schulte Fiona , Brooks Brian L. , MacAllister William S. , Désiré Naddley , Mineyko Aleksandra , Vasserman Marsha , Leaker Michael T. , Truong Tony H. , Shah Ravi , Lewis Victor A. , Yeates Keith Owen , Guilcher Gregory M. T. 
Published: July 2022

AbstractBackground/objectivesDespite advances in the treatment of sickle cell disease (SCD), cerebrovascular and cognitive insults can have lifelong consequences. Hematopoietic cell transplantation (HCT) is an established curative therapy, and recent studies have demonstrated efficacy with reduced toxicity nonmyeloablative (NMA) regimens, but little is known about neuropsychological outcomes. The objective of this study was to describe neuropsychological, behavioral, and quality‐of‐life outcomes with medical correlates in children with SCD who received an NMA matched sibling donor (MSD) HCT.Design/methodsRetrospective cohort analysis of nine recipients with hemoglobin SS SCD who underwent MSD HCT using the National Institutes of Health (NIH) NMA protocol.ResultsMean full‐scale intellectual functioning (FSIQ) was average pre‐HCT (FSIQ = 92.1, SD 9.0; n = 8) and 2 years post‐HCT (mean FSIQ = 96.6; SD 11.1; N = 9). Neuropsychological functioning was largely average across all cognitive domains, and no pre/post‐HCT differences were found to be statistically significant given the small sample size. However, effect sizes revealed moderate improvements in processing speed (Cohen's d = .72) and verbal memory (Cohen's d = .60) post‐HCT, and declines in measures of attention (Cohen's d = −.54) and fine motor speed and dexterity (Cohen's d = −.94). Parents endorsed better quality of life (Cohen's d = .91), less impact of SCD on their family, and less worry about their child's future (Cohen's d = 1.44).ConclusionNeuropsychological functioning in a sample of children and adolescents treated uniformly with NMA MSD HCT remained stable or improved in most cognitive domains, and improvements in quality of life and family functioning were observed.
Aripiprazole sensitizes head and neck cancer cells to ionizing radiation by enhancing the production of reactive oxygen species
Authors: Jeong Hyeon‐Jeong , Jung Chan‐Woong , Kim Hee‐Jin , Park Byunghee , Moon Youna , Kim Jeong‐Yub , Park Myung‐Jin 
Published: July 2022

AbstractDrug repositioning is an alternative process for drug development in cancer. Specifically, it is a strategy for the discovery of new antitumor drugs by screening previously approved clinical drugs. On the basis of this strategy, aripiprazole, an antipsychotic drug, was found to have anticancer activity. In this study, we investigated the radiosensitizing effects of aripiprazole on head and neck cancer cells at sublethal doses of ionizing radiation (IR) in vitro and in vivo. Treatment with aripiprazole suppressed the growth of head and neck cancer cells in a concentration‐dependent manner, as evidenced by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay. Intriguingly, aripiprazole significantly enhanced the sensitivity of these cells to the IC50 dose of IR. The combination of aripiprazole with IR synergistically increased annexin and propidium iodide double‐positive and terminal deoxynucleotidyl transferase dUTP nick end labeling‐positive cell populations, and induced cleaved poly(ADP‐ribose) polymerase and caspase‐3 expression, indicating the induction of apoptosis in these cells. Aripiprazole and IR‐induced apoptosis were accompanied by an increase in reactive oxygen species and was almost completely suppressed by the addition of the antioxidant, N‐acetylcysteine. Finally, aripiprazole greatly sensitized xenograft tumors to IR at doses that did not affect tumor growth. Taken together, these results suggest that aripiprazole could be considered a potent radiosensitizer for head and neck cancer.
Influence of prazosin on systemic iron levels and the associated iron metabolic alterations in spontaneously hypertensive rats
Authors: Chang Hengrui , Zhang Dong , Xin Zhen , Zhang Pengfei , Ding Wenyuan , Chang Yan‐Zhong 
Published: July 2022

AbstractThe relationship between cardiovascular diseases and iron disorders has gained increasing attention; however, the effects of hypotensive drugs on iron metabolic alterations in hypertension are not well understood. The purpose of this study was to investigate iron metabolic changes after prazosin treatment of spontaneously hypertensive rats (SHRs) and Wistar–Kyoto (WKY) rats. Our second objective was to examine the effects of hypertension and anti‐hypertensive drugs on bone formation and resorption. SHRs and WKY rats were randomized into either prazosin‐treated groups (WKY + PZ and SHR + PZ) or untreated groups (WKY and SHR). After 7 days of intragastric prazosin administration, the rats were sacrificed for analysis; blood samples and organs (the duodenum, liver, kidneys, spleen, and femur) were collected. Both WKY + PZ and SHR groups exhibited iron deficiency in the serum and liver. Prazosin increased the iron levels in the bone tissue of SHRs. Prazosin stimulated the expression of hepcidin mRNA in the liver of SHRs and inhibited the expression of this iron‐regulatory hormone in WKY rats. FPN1 expression in the duodenum was increased significantly in SHRs, however markedly decreased after prazosin treatment. The expression of TLR4 and Ctsk was enhanced in the bone tissue of SHRs, whereas CLC‐7 expression was inhibited. Both hypotension and hypertension can lead to iron deficiency. Treatment with prazosin restored iron homeostasis in SHRs. The inverse impacts of prazosin on hepatic hepcidin expression in SHRs versus WKY rats indicates differing iron regulatory mechanisms between hypertensive and normal animals. The osteoclast activity was found to be enhanced in SHRs. Further study is needed to address whether the changes in osteoblast and osteoclast activity in SHRs correlates with the effects on iron metabolism.
Pneumatic spray delivery‐based solid set canopy delivery system for oblique banded leaf roller and codling moth control in a high‐density modern apple orchard
Authors: Sahni Ramesh K. , Ranjan Rakesh , Hoheisel Gwen‐Alyn , Khot Lav R. , Beers Elizabeth H. , Grieshop Matthew J. 
Published: July 2022

AbstractBACKGROUNDPneumatic spray delivery (PSD)‐based solid set canopy delivery systems (SSCDS) have demonstrated comparable spray deposition and reduced off‐target drift compared with axial‐fan airblast sprayers in high‐density apple orchards. An important next step is to quantify whether PSD‐based SSCDS can provide effective pest management. This study evaluated the biological efficacy of this fixed spray system variant and compared it with that of an axial‐fan airblast sprayer. Partial field trials were conducted in a commercial apple orchard (cv. Jazz) trained in tall spindle architecture. Insecticides were applied at a rate of 935 L ha−1 (100 gallons per acre) for both application systems. Twenty‐four hours after spraying, leaves and fruits were collected to prepare the laboratory bioassays enabling evaluation of efficacy against obliquebanded leafroller (OBLR) and codling moth (CM).RESULTSOBLR mortality for SSCDS, airblast sprayer and untreated control treatments after 24 h of larval exposure was 91%, 98% and 4%, respectively and increased to 98%, 100% and 19% after 48 h. First‐instar CM leaf bioassay mortality was 100% for SSCDS and airblast sprayer treatment, and 13% for the untreated control at 24 h post exposure. Larval CM mortality on fruit was 100% for SSCDS and airblast sprayer treatments, and 33% on the untreated control.CONCLUSIONSInsecticides applied using SSCDS and an airblast sprayer had comparable larval mortality in all three assays, significantly higher than the untreated controls. These results suggest that PSD‐based SSCDS may provide a viable alternative to axial‐fan airblast sprayers in high‐density apple orchards. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Application of longitudinal item response theory models to modeling Parkinson’s disease progression
Authors: Zou Haotian , Aggarwal Varun , Stebbins Glenn T. , Müller Martijn L. T. M. , Cedarbaum Jesse M. , Pedata Anne , Stephenson Diane , Simuni Tanya , Luo Sheng 
Published: July 2022

AbstractThe Movement Disorder Society revised version of the Unified Parkinson’s Disease Rating Scale (MDS‐UPDRS) parts 2 and 3 reflect patient‐reported functional impact and clinician‐reported severity of motor signs of Parkinson’s disease (PD), respectively. Total scores are common clinical outcomes but may obscure important time‐based changes in items. We aim to analyze longitudinal disease progression based on MDS‐UPRDS parts 2 and 3 item‐level responses over time and as functions of Hoehn & Yahr (H&Y) stages 1 and 2 for subjects with early PD. The longitudinal item response theory (IRT) modeling is a novel statistical method addressing limitations in traditional linear regression approaches, such as ignoring varying item sensitivities and the sum score balancing out improvements and declines. We utilized a harmonized dataset consisting of six studies with 3573 subjects with early PD and 14,904 visits, and mean follow‐up time of 2.5 years (±1.57). We applied both a unidimensional (each part separately) and multidimensional (both parts combined) longitudinal IRT models. We assessed the progression rates for both parts, anchored to baseline H&Y stages 1 and 2. Both the uni‐ and multidimensional longitudinal IRT models indicate significant worsening time effects in both parts 2 and 3. Baseline H&Y stage 2 was associated with significantly higher baseline severities, but slower progression rates in both parts, as compared with stage 1. Patients with baseline H&Y stage 1 demonstrated slower progression in part 2 severity compared to part 3, whereas patients with baseline H&Y stage 2 progressed faster in part 2 than part 3. The multidimensional model had a superior fit compared to the unidimensional models and it had excellent model performance.
Extracting daily isotopic records on fish otolith (Trachurus japonicus) by combining micro‐milling and micro‐scale isotopic analysis (MICAL‐CF‐IRMS)
Authors: Muto Daichi , Ishimura Toyoho , Takahashi Motomitsu , Nishida Kozue 
Published: July 2022

RationaleThe recent progress in micro‐scale isotopic analytical techniques for otoliths has enabled the reconstruction of the experienced water temperature history of fish in every few days resolution using the stable oxygen isotope ratio (δ18O) of otoliths. We aimed to improve those techniques and extract the daily δ18O records of otoliths formed during the juvenile period.MethodsGrowth rings were formed daily in fish otoliths. We precisely distinguished the daily rings in otoliths of Japanese jack mackerel Trachurus japonicus, and milled them along daily growth rings using a high‐spatial resolution micromilling system (Geomill326). Then, we determined the stable carbon and oxygen (δ13C, δ18O) isotopic compositions using a high‐precision micro‐scale isotopic analytical system (MICAL3c with IsoPrime 100).ResultsWe successfully milled each daily ring with width ranging from 14.0 to 62.9 μm (average 27.0 μm) during the high growth period (30–70 days after hatching), and determined the isotopic compositions of otolith aragonite.ConclusionsOur improved micro‐scale analytical method is the first to determine the daily δ18O history of fish otoliths. By using our method together with the δ18O – water temperature equation, the daily history of experienced water temperature can be elucidated. Our high‐resolution milling and analytical technique can also be applied to high‐resolution isotope analysis for stalactites, clams, and corals.
Phylogenomics of the Caribbean melocacti: Cryptic species and multiple invasions
Authors: Majure Lucas C. , Barrios Duniel , Díaz Edgardo , Bacci Lucas F. , Piñeyro Yuley Encarnación 
Published: July 2022

AbstractThe widespread Neotropical genusMelocactusof approximately 42 currently recognized species, is most diverse in eastern Brazil and the Greater Antilles, especially Cuba. Species delimitation is notoriously problematic in the group, although this is due in part to a lack of detailed systematic studies, as well as a severely cluttered nomenclatural history. To date, no comprehensive phylogenetic hypotheses have been generated for the clade, although some population genetic and morphological studies exist. We generated the largest phylogenetic dataset ofMelocactusto date based on plastome data derived from a genome‐skimming approach for 26 taxa, which provided a framework for understanding species limits and relationships among Caribbean species. Our time‐calibrated phylogeny revealed a mid‐Pleistocene origin forMelocactus, and we resolved three major clades, a Cuban clade, a mostly South American clade, and a widespread Caribbean clade, which also included some South American taxa. Our topology recovered the Cuban clade as sister to the rest of the species, although this placement was poorly supported, and several other Cuban species are scattered throughout the rest of the tree. Biogeographic analyses suggested multiple dispersal events from South America leading to the current diversity on Cuba, as well as other parts of the Antilles. Based on our phylogenetic results, previous hypotheses of species numbers and relationships in the Caribbean generated solely on morphology have, in some cases, been greatly underestimated. Our study shows that plastome data are effective for resolving clades and species limits inMelocactus, although future work will need to include broader sampling and larger datasets to fully resolve relationships in this complicated group of cacti. We describe one new cryptic species for Cuba,Melocactus santiagoensissp. nov., and provide a new combination (Melocactus lagunaensiscomb. & stat. nov.), based on our phylogenetic results and morphological data and typify numerous names in the genus. The genusMelocactusis another striking example of the exceptional diversity that has been generated in the poorly studied, seasonally dry tropical forest of the Greater Antilles.
Caecal dysfunction following standing surgical procedures
Authors: Gough Rachel L , McGovern Kate F , Bladon Bruce M , Carmichael Lucy AJ 
Published: July 2022

AbstractThe aim of this study is to report cases of caecal dysfunction following surgical procedures in the standing horse. The study design is retrospective. Six client‐owned horses developed caecal dysfunction following a variety of surgical procedures undertaken in the standing sedated horse. Medical records were reviewed for caecal dysfunctions that had occurred in horses within 2 weeks of standing surgical procedures. Signalment, details of the original standing surgery and medications administered were recorded. Short‐term outcome was obtained from clinical records. Long‐term outcome was obtained by telephone questionnaire with the owner. Six horses were identified to have developed caecal dysfunction following standing surgery for tooth extraction, laparoscopic ovariectomy, laparoscopic cryptorchidectomy, fracture repair, melanoma removal and castration. Three horses were euthanised with caecal perforation at the time of diagnosis. Three underwent surgical treatment (typhlotomy, decompression and caecal bypass by ileocolostomy). All three horses were alive post‐operatively (follow‐up at 2, 12 and 24 months). Caecal dysfunction may develop following surgical procedures performed under standing sedation. Careful post‐operative monitoring and early identification of caecal dysfunction, and consequent potential need for surgical intervention, are important to optimise outcomes and minimise the risk of fatal caecal perforation occurring.
Effect of processed sweet lupin (Lupinus angustifolius) grain supplementation on growth performance and socioeconomic feasibility of Doyogena sheep in Ethiopia
Authors: Tilaye Habite , Asmare Bimrew , Meheret Fentahun , Bezabih Melkamu , Jane Wamatu 
Published: July 2022

AbstractBackgroundThe experiment evaluated the effect of supplementing sheep fed natural pasture hay withprocessed sweet lupin grain on growth performance and its economic feasibility. The finding revealed that use of steamed lupin shown to improve the nutritivevalue of the grain and sheep performance.MethodsThe experiment was carried out using 24 yearling lambs with initial body weight of 27.53 ± 2.67 kg (mean ± SD) for 126 days (21 days quarantine, 15 days of adaptation and 90 days growth trial followed by 7 days digestibility trial). The experiment was laid out in a randomised complete block design consisting of four treatments and six blocks. Treatments comprised the feeding of natural pasture hay ad libitum + concentrate mix 440 g (T1), natural pasture hay + 440 g/day roasted, coarsely ground sweet lupin grain (T2), natural pasture hay + 440 g/day sweet lupin grain soaked in water for 72 h (T3), natural pasture hay + 440 g/day steamed sweet lupin grain (T4).ResultsThere was improvements in total dry matter intake and digestibility coefficients of dry matter, organic matter, crude protein, neutral detergent fibre and acid detergent fibre in sheep supplemented with processed sweet lupin grains compared (T4) by 58.49%, 24.66%, 39.39%, 22.97% and 39.68%, respectively, over the control group. Specifically sheep supplemented with T4 had significantly higher (p < 0.001) average daily gain (by 51.04%), feed conversion efficiency (46.34%) and daily weight gain (144.78 g/day) compared to the control treatment, respectively. All processing methods resulted in favourable average daily gain and net return, thus can be employed in feeding systems depending on their availability and relative cost.ConclusionsSupplementing sheep fed natural pasture hay with 440 g/day steamed sweet lupin grains improved growth performance and fattening economics of Doyogena sheep compared to T2 (roasted sweet lupin grain), T3 (soaked sweet lupin grain) or the control (T1).
Three‐dimensional in vitro maturation of rabbit oocytes enriched with sheep decellularized greater omentum
Authors: Fazelian‐Dehkordi Khatereh , Talaei‐Khozani Tahereh , A S. Fakhroddin Mesbah 
Published: July 2022

AbstractBackgroundTo prevent ovarian hyperstimulation syndrome, in vitro maturation (IVM) allows the oocytes for infertility treatment without hormone therapy. Although many oocytes matured during IVM, some deficiencies in the culture conditions lead to inhibition of the growth and development of the cumulus cells and the oocyte nuclear and cytoplasmic maturation.ObjectivesThe challenge of improving the oocyte culture conditions prompted us to use greater omentum (GOM), full of growth factors and proteins, as a rich supplement to the base culture medium.MethodsCumulus‐oocyte complexes were recovered from rabbits and divided into 3D and 2D conditions cultured for 12 and 24 h. In 3D cultures, the oocytes embedded in alginate containing FBS decellularized GOM. Corresponding supplements were also added in 2D conditions—maturation of the oocytes evaluated by Aceto‐Orcein, TEM, and RT‐PCR for MAP2K1 and Cdk2.ResultsDNA quantification, Hoechst, and H&E staining confirmed cell depletion from GOM, and SEM showed the preservation of ultra‐architecture after decellularization. Histochemical staining methods showed appropriate extracellular matrix preservation. ELISA assessment showed retention of VEGF content. MTT assessment indicated decellularized GOM was non‐toxic. Both Aceto‐Orcein assessment and ultra‐structure study of the oocytes showed that supplementation of 2D or 3D cultures with decellularized omentum promoted oocyte maturation. Expression of MAP2K1 and Cdk2 also increased in the presence of GOM.ConclusionsGOM supplementation has a beneficial impact on oocyte maturation, probably due to the presence of growth factors and proteins.
Vertical Land Motion at Tide Gauges Observed by GNSS: A New GFZ-TIGA Solution
Authors: Männel Benjamin , Schöne Tilo , Bradke Markus , Schuh Harald 
Published: July 2022

Abstract
Long-term tide gauge records provide valuable insights to sea level variations but interpretation requires an accurate determination of associated vertical land motion. Within the Tide Gauge Benchmark Monitoring Pilot Project of the International GNSS Service dedicated reprocessing campaigns are performed for GNSS stations co-located with tide gauges. Based on 341 stations the GFZ contribution to the third TIGA reprocessing provides vertical land motion rates for 230 stations at or close to recently active tide gauges. GNSS station coordinate time series determined by using a network approach and a conventional time series analysis show mean repeatabilities of 2.9, 3.3, and 5.6 mm for north, east, and up coordinates. The derived vertical velocity pattern is analyzed but also compared to the ALTIGAPS and the ULR6a solutions showing mean differences of 0.04 mm yr−1 and −0.1 mm yr−1, respectively. By correcting tide gauge records available via PSMSL for the individual vertical station velocity including eventually velocity changes geocentric sea level changes are determined. Compared to AVISO’s multi-mission altimetric trend map a difference of −0.7 mm yr−1 is determined.
Folk Fish Names Are Condensed Forms of Traditional Knowledge: Case Study with the Vaie People of Sarawak, Malaysia
Authors: Hidayati Syafitri , Franco F. Merlin , Abdul Ghani Bibi Aminah , Giridharan Beena , Hassan Mohd Zafri 
Published: July 2022

AbstractFolk names that form the backbone of folk biological classifications are coined using traditional knowledge and linguistic mechanisms. In the process, traditional knowledge on the salient characteristics of the denoted taxa gets encoded in their respective names. In this chapter, we present 132 folk names used by the Vaie community of Sarawak, Malaysia to denote the fish known to them. Based on the apparency of traditional knowledge encoded by these names, we classify them into barefaced and cryptic. We found 61 Vaie fish names encoding traditional knowledge related to ecology, morphology, quality, and utility values of the respective taxa. Of these, 21 names were found to be cryptic (metaphors—11; metonyms—10), and the rest barefaced. Cryptic names such as metonyms and metaphors often encode traditional knowledge that spans beyond the taxon denoted. Folk names encoding traditional knowledge are indicative of the larger body of traditional knowledge held by local communities.