bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penehyclidine Hydrochloride Protects Rat Cardiomyocytes from Ischemia-
Reperfusion Injury by Platelet-derived Growth Factor-B
Authors: Lu Yan , Zi Congna , Zhang Liang , Cui Boqun , Li Ling , Ma Jun 
Published: July 2022

Aims and Objective:
The lack of effective treatments for myocardial ischemiareperfusion
(MI-R) injury severely restricts the effectiveness of the treatment of ischemic heart
disease. In the present research, we aimed to investigate the protective effect and molecular mechanism
of penehyclidine hydrochloride (PHC) on MI-R cells.

Method:
Cell viability was quantified using CCK8. Cell apoptosis was analyzed using flow cytometry.
Western blot and Elisa assays were used for the detection of target proteins.

Result:
PHC pretreatment attenuated the inhibition of cell viability and decreased the percentage
of apoptosis induced by simulated ischemia reperfusion (SIR). Platelet-derived growth factor B
(PDGF-B) and its downstream AKT pathway were activated in PHC pretreated cells. After siRNAPDGF-
B transfection, cell viability was inhibited and apoptosis was activated in PHC pretreated
SIR cells, suggesting that PHC protected cells from SIR. PDGF-B knockdown also increased the
levels of CK, LDH, IL-6 and TNF-α in PHC pretreated SIR cells. The effect of AKT inhibitor on
H9C2 cells was consistent with that of PDGF-B knockdown.

Conclusion:
PHC pretreatment can protect cardiomyocytes from the decrease of cell activity and
the increase of apoptosis caused by reperfusion through up-regulating PDGF-B to activate PI3K
pathway. Our study indicates that PHC is a potential drug to protect cells from reperfusion injury
and PDGF-B is a potential target for preventing MI-R injury.
Extracellular Vesicles Derived from Adipose-Derived Stem Cells Facilitate
Frostbite Wound Healing By Regulating SOCS3 Expression
Authors: Zhang Nan , Yu Xin , Li Wei , Zhang Kai , Yu Jiaao , Liu Tongjun 
Published: July 2022

Background:
Though adipose-derived stem cells (ADSCs) have potential applications for the
repair and regeneration of damaged tissues, limited studies have defined the function of ADSCs on dermal
fibroblasts. Our RNA-seq sequencing identified differentially expressed SOCS3 in frostbite injury.

Objective:
In the current study, we aim to examine the hypothesis that extracellular vesicles derived from
adipose-derived mesenchymal stem cells (ADSCs-EVs) may modulate SOCS3/TGF-β1 signaling in
wound healing of frostbite injury.

Methods:
sh-SOCS3 and sh-TGF-β1 were introduced to explore the biological role of SOCS3 in frostbite
injury by detecting the proliferation and migration of human skin fibroblast (HSF) cells and the wound
healing in mice. Furthermore, the extracted ADSCs-EVs were interfered with HSF cells in vitro or injected
into the frostbitten mouse model in vivo.

Results:
Upregulation of SOCS3 occurred in the skin tissues of frostbitten mice. Compared to sh-NC, the
wound healing rate of sh-SOCS3 presented higher on day 7(31.34±4.35 vs 41.83±3.74, p < 0.05) and day
14 (63.42±6.01 vs 88.99±5.12, p < 0.05) after injury. Silencing SOCS3 can promote frostbite wound healing.
Moreover, SOCS3 downregulated TGF-β1 to suppress the proliferation and migration of HSF cells, thus
impeding the skin wound healing. Additionally, ADSCs-EVs could enhance the proliferation and migration
of HSF cells according to the results of CCK-8 assay (p < 0.05), scratch test (17.82±4.25 vs
49.78±2.54, p < 0.05) and Transwell assay (42.33±6.81 vs 91.33±7.02, p < 0.05), and regulate the expression
of SOCS3/TGF-β1. The role of ADSCs-EVs in frostbite wound healing was also confirmed in vivo.
ADSCs-EVs could promote frostbite wound healing by downregulating the expression of SOCS3 and
upregulating the expression of TGF-β1 and collagen I.

Conclusions:
Collectively, ADSCs-EVs inhibit SOCS3 and facilitate the expression of TGF-β1, which
promotes the proliferation and migration of HSF cells and subsequently enhances wound healing of frostbite
injury.
Inducing and Recording Acute Stress Responses on a Large Scale With the Digital Stress Test (DST): Development and Evaluation Study
Authors: Norden Matthias , Hofmann Amin Gerard , Meier Martin , Balzer Felix , Wolf Oliver T , Böttinger Erwin , Drimalla Hanna 
Published: July 2022

Background
Valuable insights into the pathophysiology and consequences of acute psychosocial stress have been gained using standardized stress induction experiments. However, most protocols are limited to laboratory settings, are labor-intensive, and cannot be scaled to larger cohorts or transferred to daily life scenarios.

Objective
We aimed to provide a scalable digital tool that enables the standardized induction and recording of acute stress responses in outside-the-laboratory settings without any experimenter contact.

Methods
On the basis of well-described stress protocols, we developed the Digital Stress Test (DST) and evaluated its feasibility and stress induction potential in a large web-based study. A total of 284 participants completed either the DST (n=103; 52/103, 50.5% women; mean age 31.34, SD 9.48 years) or an adapted control version (n=181; 96/181, 53% women; mean age 31.51, SD 11.18 years) with their smartphones via a web application. We compared their affective responses using the international Positive and Negative Affect Schedule Short Form before and after stress induction. In addition, we assessed the participants’ stress-related feelings indicated in visual analogue scales before, during, and after the procedure, and further analyzed the implemented stress-inducing elements. Finally, we compared the DST participants’ stress reactivity with the results obtained in a classic stress test paradigm using data previously collected in 4 independent Trier Social Stress Test studies including 122 participants overall.

Results
Participants in the DST manifested significantly higher perceived stress indexes than the Control-DST participants at all measurements after the baseline (P<.001). Furthermore, the effect size of the increase in DST participants’ negative affect (d=0.427) lay within the range of effect sizes for the increase in negative affect in the previously conducted Trier Social Stress Test experiments (0.281-1.015).

Conclusions
We present evidence that a digital stress paradigm administered by smartphone can be used for standardized stress induction and multimodal data collection on a large scale. Further development of the DST prototype and a subsequent validation study including physiological markers are outlined.
Assessing Social Media Data as a Resource for Firearm Research: Analysis of Tweets Pertaining to Firearm Deaths
Authors: Singh Lisa , Gresenz Carole Roan , Wang Yanchen , Hu Sonya 
Published: July 2022

BackgroundHistoric constraints on research dollars and reliable information have limited firearm research. At the same time, interest in the power and potential of social media analytics, particularly in health contexts, has surged.ObjectiveThe aim of this study is to contribute toward the goal of establishing a foundation for how social media data may best be used, alone or in conjunction with other data resources, to improve the information base for firearm research.MethodsWe examined the value of social media data for estimating a firearm outcome for which robust benchmark data exist—specifically, firearm mortality, which is captured in the National Vital Statistics System (NVSS). We hand curated tweet data from the Twitter application programming interface spanning January 1, 2017, to December 31, 2018. We developed machine learning classifiers to identify tweets that pertain to firearm deaths and develop estimates of the volume of Twitter firearm discussion by month. We compared within-state variation over time in the volume of tweets pertaining to firearm deaths with within-state trends in NVSS-based estimates of firearm fatalities using Pearson linear correlations.ResultsThe correlation between the monthly number of firearm fatalities measured by the NVSS and the monthly volume of tweets pertaining to firearm deaths was weak (median 0.081) and highly dispersed across states (range –0.31 to 0.535). The median correlation between month-to-month changes in firearm fatalities in the NVSS and firearm deaths discussed in tweets was moderate (median 0.30) and exhibited less dispersion among states (range –0.06 to 0.69).ConclusionsOur findings suggest that Twitter data may hold value for tracking dynamics in firearm-related outcomes, particularly for relatively populous cities that are identifiable through location mentions in tweet content. The data are likely to be particularly valuable for understanding firearm outcomes not currently measured, not measured well, or not measurable through other available means. This research provides an important building block for future work that continues to develop the usefulness of social media data for firearm research.
Análisis del ciclo de vida (ACV): De un cemento producido con reemplazo de cáscara de huevo y cáscara de arroz
Authors: Frías-Gutiérrez Steven , Revuelta-Muñoz Mauricio , Pacheco-Bustos Carlos 
Published: July 2022

Introducción. La industria cementera es una de las causantes del cambio climático, por lo que las investigaciones actualmente están enfocadas en utilizar materiales de desecho que puedan reducir la cantidad de materia prima que se extraiga para su fabricación. Así como también es necesario evaluar los daños ambientales asociados a estos nuevos productos que garanticen la reducción de los efectos negativos. Objetivo. Evaluar los impactos ambientales, utilizando el análisis del ciclo de vida (ACV) basado en la norma ISO 14040, ocasionados por una cadena de producción de cemento Portland con un reemplazo de 14 % de cáscara de huevo y 5 % de cáscara de arroz, manteniendo los procesos actuales como molienda e incineración con el mismo consumo energético. Materiales y métodos. Para el análisis del inventario se tuvo en cuenta la base de datos de Agribalyse v3.0.1, la cual es un un repositorio francés para el análisis de ACV del sector agrícola y alimentario, estas inmersas en el software a usar como es OpenLCA. Para esto el cemento con porcentaje de reemplazo de cáscara de huevo (14 %) y cascarilla de arroz (5 %) se requiere: • Cáscara de huevo y cascarilla de arroz. • Insumos energéticos de sistemas externos como el carbón y la electricidad. • Piedra Caliza, Arena y Yeso. • Transporte de materiales Y generan ambos como salidas un cemento Portland con reemplazo de 14 % de CH y 5 % de CA (para 1 tonelada de cemento). Resultados. Es posible observar que la industria con mayor aporte al cambio climático es la relacionada con la producción de energía en forma de combustión, la cual aporta un total de 398,10 kg de CO2 equivalente por cada tonelada de cemento con reemplazo de 14 % de CH y 5 % de CA, este proceso es relacionado durante la etapa de producción en los procesos de incineración. Así mismo, la segunda industria con más aporte al cambio climático es la industria minera, la cual es responsable de la liberación 200,10 kg de CO2 equivalente por cada tonelada de este tipo de cemento. Conclusiones. Mediante este estudio se pudo constatar que el uso de reemplazo de 14 % de CH y 5 % de CA en un cemento Portland y su incorporación en el proceso actual, implica una disminución en todos los impactos ambientales asociados. Ya que, a pesar de se generen nuevos efectos asociados a la producción del arroz, estos siempre estarán presentes debido a que es un producto alimentario de primera necesidad que genera gran cantidad de residuos al no disponer adecuada su cáscara, por lo que adicionarla junto a otro desecho como las cáscaras de huevo implica una mejora encaminada a la economía circular.
Economía, Comercio Digital y el Consumo del Ciudadano de Lima Metropolitana
Authors: Vargas Salazar Ivonne , Motta Flores Madalyne , Escudero Cavero Benjamín , Barrientos-Felipa Pedro 
Published: July 2022

Introducción. Se exponen los resultados de la investigación compatibilizando hallazgos y conceptos identificados en la economía. Objetivo. Identificar el contexto en que se desarrolla la economía digital, su avance e impacto en la economía peruana específicamente en la ciudad de Lima, y su relación con el consumo. Materiales y métodos. El enfoque de investigación es cuanti-cualitativo con un alcance descriptivo. Se aplican dos cuestionarios, estructurado para usuarios y no estructurado para expertos. Resultados. La aplicación de cuestionarios y compatibilización con datos secundarios, exponen la acelerada aplicación de tecnologías de información y comunicación y el fortalecimiento de la economía digital. El comercio electrónico relacionado impulsa el desarrollo de la bancarización de los consumidores. La tendencia de los países hacia la economía digital requiere modificaciones sociales y normativas para aprovechar la tecnología. Los consumidores de Lima Metropolitana el 58,4 % reconocen su importancia para la sociedad, pero solo el 6,0 % destina más del 50 % del presupuesto en compras en línea. Conclusiones. El desarrollo de la economía digital y el comercio electrónico favorece a la sociedad con menores costos, diversidad de oferta y ahorro de tiempo en desplazamiento físico. Se necesitan políticas que promuevan la inclusión e igualdad de acceso. En los consumidores de Lima Metropolitana existe posibilidad de desarrollo del comercio electrónico y limitaciones.
Prevalence and risk factors for acquired long QT syndrome in the emergency department: a retrospective observational st
Authors: Fernandes Diogo Filipe , Camões Guilherme , Ferreira Diana , Queijo Carolina , Fontes-Ribeiro Carlos , Gonçalves Lino , Pina Rui , António Natália 
Published: July 2022

Purpose: Long QT syndrome (LQTS) is a rare heterogeneous syndrome that
may be congenital or, more frequently, acquired (aLQTS). The real-world
prevalence of acquired LQTS in the emergency department is unknown.
Methods: The aim of this study was to determine the prevalence of this
syndrome in the emergency room and to identify risk factors.
Electrocardiograms (ECG) of 5056 consecutively patients admitted in the
ER of a tertiary hospital between January 28 and
March 17 of 2020 were reviewed. All patients with
aLQTS were included. Clinical data with a focus on QT prolonging drugs
and clinical factors were recorded. Statistical comparison was made
between the groups with and without corrected QT interval greater than
500ms (value that is considered severely increased).
Results: A total of 383 ECGs with prolonged QTc were recognized,
corresponding to a prevalence of aLQTS at admission of 7.81%. Patients
with aLQTS were more commonly men (53.3%) with an age of 73.49±14.79
years old and QTc interval of 505.3±32.4ms. Only 20,4% of these
patients with aLQTS were symptomatic. No ventricular arrhythmias were
recorded. Patients with QT interval greater than 500ms were more
frequently female (59,5%; p<0.001) and were more frequently
on QT prolonging drugs (77,3%; p=0.025). Main contributing factor was
intake of antibiotics (OR 4.680) followed by female gender (OR 2.473)
and antipsychotics (OR 1.925).
Conclusions: aLQTS is particularly prevalent in the ER. Female patients
on antibiotics and antipsychotics are at particularly high risk. Efforts
must be made to avoid, detect and treat aLQTS as early as possible.
Changes in visual impairment due to diabetic retinopathy during 1980–2019 based on nationwide register data
Authors: Purola Petri K.M. , Ojamo Matti U.I. , Gissler Mika , Uusitalo Hannu M.T. 
Published: July 2022

<p> </p>
<p><strong>Objective:</strong> To evaluate changes in the incidence, prevalence, severity, and onset age of visual impairment (VI) due to diabetic retinopathy (DR) and compare these trends in the screening and treatment of diabetes during 40 years based on Finnish national register data.</p>
<p><strong>Research Design and Methods:</strong> We included visually impaired persons with non-proliferative DR (NPDR; <em>n</em> = 2490, 73% females) or proliferative DR (PDR; <em>n</em> = 2026, 53% females) as the main diagnosis for VI during 1980–2019 in the Finnish Register of Visual Impairment. Number of treated diabetes patients during 1986–2019 was obtained from the Social Insurance Institution of Finland registers based on reimbursed medication data.</p>
<p><strong>Results:</strong> Annual incidence of reported VI due to DR has decreased since it peaked in the 1990s: regarding NPDR, it decreased from 102.3 to 5.5 per 100 000 treated diabetes patients between the 1990s and 2010s; regarding PDR, respective change was from 39.9 to 7.4. The incidence of diabetes patients treated for DR increased during this period. Annual prevalence of reported VI and differences between sexes steadily decreased in the 2000s and 2010s. The severity of reported VI has decreased and the age at the onset of reported VI increased during the 40 years.</p>
<p><strong>Conclusions: </strong>Prevalence and incidence of VI due to DR have dramatically decreased and shifted to older age during the 40 years despite the increasing prevalence of diabetes. These positive trends highlight the successful development and effectiveness of screening and therapies of diabetes and DR.</p>
Maternal and Paternal Exercise Induce Distinct Metabolite Signatures in Offspring Tissues
Authors: Hernández-Saavedra Diego , Markunas Christina , Takahashi Hirokazu , Baer Lisa A. , Harris Johan E. , Hirshman Michael F. , Ilkayeva Olga , Newgard Christopher B. , Stanford Kristin I. , Goodyear Laurie J. 
Published: July 2022

<p> </p>
<p>Maternal and paternal exercise are well-established to improve the metabolic health of adult offspring. Tissue and serum metabolites play a fundamental role in the health of an organism, but how parental exercise affects offspring tissue and serum metabolites has not yet been investigated. Here, male and female breeders were fed a high-fat diet and housed with or without running wheels before breeding (males) and before and during gestation (females). Offspring were sedentary and chow-fed and had both parents sedentary (Sed); maternal exercise (MatEx); paternal exercise (PatEx); or maternal+paternal exercise (Mat+PatEx). Adult offspring from all parental exercise groups had similar improvement in glucose tolerance and hepatic glucose production. Targeted metabolomics was performed in offspring serum, liver, and triceps muscle. Offspring from MatEx, PatEx, and Mat+PatEx each had a unique tissue metabolite signature, but Mat+PatEx offspring had an additive phenotype relative to MatEx or PatEx alone in a subset of liver and muscle metabolites. Tissue metabolites consistently indicated that the metabolites altered parental exercise were consistent with enhanced fatty acid oxidation. These data identify distinct tissue-specific adaptations and mechanisms for parental exercise-induced improvement in offspring metabolic health. Further mining of this dataset could aid the development of novel therapeutic targets to combat metabolic diseases.</p>
Effects of B Addition on the Microstructure and Microhardness of Melt-Spun Al-7075 Alloy
Authors: Kilicaslan Muhammed Fatih , Elburni Saad Ibrahim , Yilmaz Yasin , Akkaş Mehmet 
Published: July 2022

Abstract
Main aim of this study is to examine change of microstructural and the mechanical properties of Al-7075 alloy depending on B content added with different proportions. Rapid solidification process used to produce the alloys was carried out with a single roll melt spinner via a wheel with a rotational speed of 25 m/s. For the microstructural characterization of the alloys, a scanning electron microscope and X-ray diffraction analyzes were used. According to obtained results, it can be said that B addition led to modification of dimensions and shapes of both α−Al and intermetallic phases occurred, it reduced average grain size from 0.45 μm to 0.34 μm in the microstructure. The B addition also led to dramatically increase in microhardness of the Al-7075 alloy. The microhardness of the alloy with 0.4 wt. % B is 0.19 GPa, this is clearly one times higher than that of the alloy without B addition. The microhardness of the alloy with 1 wt. % B is 0.21 GPa, this is also slightly higher than that of the alloy with 0.4 wt. % B.
Effect of Shop-Primer Coating on S235JR Steel on MAG Weldability
Authors: Çetinkaya Cemil , Akay Ali , Arabacı Uğur , Özdemir Uğur 
Published: July 2022

Abstract
In this study, S235JR structural steel samples in uncoated condition and coated (shop-primer) in different thicknesses were welded by MAG (Metal Active Gas) welding method, and the effects of these applications on the mechanical and microstructure properties of the material were investigated. In the experimental studies, the first specimen group were used without any sandblasting and coating application, the second group specimens were sandblasted at Sa 2 ½ degree, and 25 µm, 50 µm, and 75 µm coatings were applied to the specimens in the other group. Surface conditions and coating thicknesses were selected as the variable parameters. With the examination of the radiography films, it was observed that the surface conditions affected the welded joint. As a result of the study, it was observed that altered coating thicknesses caused defects in the welding zone. It was determined that the coating thickness partially affected the mechanical properties, and the highest hardness values occurred in the weld zone in all samples. Relatively low values were obtained in tensile, bending and Charpy impact tests performed on sample groups with 75 µm coating thickness. The results were verified by the surface fracture, SEM, and EDS studies.
Ethanolic extract of Edamame (Glycine max L. merril) enhance second degree burn wound healing trough modulating of hydroxiproline levels and increasing epithelial thickness
Authors: Sutejo Ika Rahmawati , Hasanah Arifah Nur , Sudarko Faizah Ramadhani 
Published: July 2022

Abstract

Objective: Burns has become a global health problem because it causes about 180.000 deaths worldwide every year. In developing countries, silver sulfadiazine cream is usually used as drug management for second-degree burns, but it is expensive, so several herbal treatments have developed recently. The seed of edamame has genistein compounds that can increase collagen synthesis. The antioxidant component also enhances the burn wound healing process. The study aims to prove the effectiveness of ethanolic extract of edamame’s seed in the second-degree burn wound healing process.

Methods: Burn wound was made by applying hot aluminum (70 °C) coin on the skin rat. The negative control group (C-) was given Na-CMC 0.5%, the positive control group (C+) was given cream silver sulfadiazine, the treatment groups T1, T2, T3, and T4 were given an ethanolic extract of edamame with the concentration of 20%, 40%, 60%, and 80% for 15 days. Hydroxyproline levels were evaluated by hydroxyproline biochemistry assay, the epithelial thickness was observed on histopathological preparations with HE staining.

Results: The results showed that the hydroxyproline levels on the 16th were modulated, while epithelial thickness was higher in the treatment group than in the control group (P<0.05).

Conclusions: We conclude that the certain concentration of ethanolic extract of edamame (Glycine max L. Merril) was effectively enhanced the second-degree burn wound healing by modulating hydroxyproline levels and increasing epithelial thickness.