bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enzyme Selection, Optimization, and Production toward Biodegradation of Waste Poly(ethylene terephthalate) at Scale
Authors: XIE DONGMING , Soong Ya-Hue , Abid Umer , Chang Allen C. , Ayafor Christian , Patel Akanksha , Xu Jin , Lawton Carl W. , Wong Hsi-Wu , J Margaret Sobkowicz 
Published: December 2022

Poly(ethylene terephthalate) (PET) is one of the world’s most widely
used polyester plastics. Due to its chemical stability, PET is extremely
difficult to hydrolyze in a natural environment. Recent discoveries in
new polyester hydrolases and breakthroughs in enzyme engineering
strategies have inspired enormous research on biorecycling of PET. This
study summarizes our research efforts toward large-scale, efficient, and
economical biodegradation of waste PET, including PET hydrolase
selection and optimization, high-yield enzyme production, and
high-capacity enzymatic degradation of waste PET. First, genes encoding
PETase and MHETase from Ideonella sakaiensis and the ICCG variant
of leaf-branch compost cutinase (LCC) were codon-optimized and expressed
in Escherichia coli BL21(DE3) for high-yield production. To
further lower the enzyme production cost, a pelB leader sequence
was fused to LCC so that the enzyme can be secreted into the
medium to facilitate recovery. To help bind the enzyme on the
hydrophobic surface of PET, a substrate-binding module in a
polyhydroxyalkanoate depolymerase from Alcaligenes faecalis (PBM)
was fused to the C-terminus of LCC. The resulting four different
LCC variants (LCC, PelB-LCC, LCC-PBM, and PelB-LCC-PBM), together with
PETase and MHETase, were compared for PET degradation efficiency. A
fed-batch fermentation process was developed to produce the target
enzymes up to 1.2 g/L. Finally, the best enzyme, PelB-LCC, was selected
and used for the efficient degradation of 200 g/L recycled PET in a
well-controlled, stirred-tank reactor. The results will help develop an
economical and scalable biorecycling process toward a circular PET
economy.
Alterations in Functional Connectivity of Thalamus and Primary Somatosensory Cortex in Painful and Painless Diabetic Peripheral Neuropathy
Authors: Croosu Suganthiya S. , Røikjer Johan , Mørch Carsten D. , Ejskjaer Niels , Frøkjær Jens B. , Hansen Tine M. 
Published: December 2022

OBJECTIVEIn this study we aimed to investigate the functional connectivity of brain regions involved in sensory processing in diabetes with and without painful and painless diabetic peripheral neuropathy (DPN) and the association with peripheral nerve function and pain intensity.RESEARCH DESIGN AND METHODSIn this cross-sectional study we used resting-state functional MRI (fMRI) to investigate functional brain connectivity of 19 individuals with type 1 diabetes and painful DPN, 19 with type 1 diabetes and painless DPN, 18 with type 1 diabetes without DPN, and 20 healthy control subjects. Seed-based connectivity analyses were performed for thalamus, postcentral gyrus, and insula, and the connectivity z scores were correlated with peripheral nerve function measurements and pain scores.RESULTSOverall, compared with those with painful DPN and healthy control subjects, subjects with type 1 diabetes without DPN showed hyperconnectivity between thalamus and motor areas and between postcentral gyrus and motor areas (all P ≤ 0.029). Poorer peripheral nerve functions and higher pain scores were associated with lower connectivity of the thalamus and postcentral gyrus (all P ≤ 0.043). No connectivity differences were found in insula (all P ≥ 0.071).CONCLUSIONSHigher functional connectivity of thalamus and postcentral gyrus appeared only in diabetes without neuropathic complications. Thalamic/postcentral gyral connectivity measures demonstrated an association with peripheral nerve functions. Based on thalamic connectivity, it was possible to group the phenotypes of type 1 diabetes with painful/painless DPN and type 1 diabetes without DPN. The results of the current study support that fMRI can be used for phenotyping, and with validation, it may contribute to early detection and prevention of neuropathic complications.
Effectiveness of Nonmydriatic Ultra-Widefield Retinal Imaging to Screen for Diabetic Eye Disease: A Randomized Controlled Trial (Clearsight)
Authors: Liu Selina L. , Gonder John R. , Owrangi Ehsan , Klar Neil S. , Hramiak Irene M. , Uvarov Artem , Mahon Jeffrey L. 
Published: December 2022

OBJECTIVE
Suboptimal diabetic eye disease screening is a major cause of preventable vision loss. Screening barriers include mydriasis and the need for dedicated screening appointments. The Clearsight trial assessed whether nonmydriatic ultra-widefield (NM UWF) screening on the day of a diabetes clinic visit improved detection of clinically important eye disease versus usual screening.

RESEARCH DESIGN AND METHODS
This single-center, randomized, parallel-group controlled trial was conducted at St. Joseph’s Health Care, London, Ontario, Canada. Adults with diabetes due for screening were randomized to same-day, on-site screening (NM UWF imaging) on the day of a scheduled diabetes clinic visit or usual screening (encouraged to arrange optometrist screening). The primary outcome was detection of actionable eye disease (AED), defined as the need for an ophthalmology referral or increased ocular surveillance. The primary analysis (modified intention-to-screen) compared the proportions of AED between groups within 1 year of enrollment.

RESULTS
Of 740 participants randomized between 7 March 2016 and 17 April 2019, 335 on-site screening and 323 usual screening participants met criteria for the primary analysis. More AED was detected in the on-site screening group than in the usual screening group (50 of 335 [14.9%] vs. 22 of 323 [6.8%]; adjusted odds ratio 2.51; 95% CI 1.49–4.36). The number needed to screen by on-site screening in order to detect 1 additional patient with AED was 13 (95% CI 8–29).

CONCLUSIONS
Same-day, on-site screening by NM UWF imaging increased the detection of clinically important diabetic eye disease versus usual screening. Integration of NM UWF imaging into routine diabetes clinic visits improved screening adherence and has the potential to prevent vision loss.
Forty Years of Behavioral Diabetes Research: A Personal Journey
Authors: Delamater Alan M. 
Published: December 2022

Editor’s noteThis article was adapted from the address Dr. Delamater delivered as the recipient of the American Diabetes Association’s Richard R. Rubin Award for 2022. This award recognizes a behavioral researcher who has made outstanding, innovative contributions to the study and understanding of the behavioral aspects of diabetes in diverse populations. Dr. Delamater delivered the address in June 2022 at the Association’s virtual 82nd Scientific Sessions. A webcast of this speech is available for viewing on the DiabetesPro website (https://professional.diabetes.org/webcast/stigma-diabetes-care%E2%80%94evidence-and-solutions-richard-r-rubin-award-lecture).
Effectiveness of non-mydriatic ultra-widefield retinal imaging to screen for diabetic eye disease: a randomized controlled trial (Clearsight)
Authors: Liu Selina L. , Gonder John R. , Owrangi Ehsan , Klar Neil S. , Hramiak Irene M. , Uvarov Artem , Mahon Jeffrey L. 
Published: December 2022

<p> </p>
<p>Objective</p>
<p>Suboptimal diabetic eye disease screening is a major cause of preventable vision loss. Screening barriers include mydriasis and need for dedicated screening appointments. The Clearsight trial assessed whether non-mydriatic ultra-widefield (NM UWF) screening on the day of a diabetes clinic visit improved detection of clinically important eye disease vs usual screening.</p>
<p>Research Design and Methods </p>
<p>This single-center, randomized, parallel-group controlled trial was conducted at St. Joseph’s Health Care, London, Canada. Adults with diabetes due for screening were randomized to same-day On-Site Screening (NM UWF imaging) on the day of a scheduled diabetes clinic visit or Usual Screening (encouraged to arrange optometrist screening). The primary outcome was detection of actionable eye disease (AED) defined as need for ophthalmology referral or increased ocular surveillance. The primary analysis (modified intention-to-screen) compared the proportions of AED between groups within one year of enrollment. </p>
<p>Results </p>
<p>Of 740 participants randomized between March 7, 2016 and April 17, 2019, 335 On-Site Screening and 323 Usual Screening participants met criteria for the primary analysis. More AED was detected in the On-Site Screening than Usual Screening groups (50/335 [14.9%] vs 22/323 [6.8%]; adjusted odds ratio 2.51 [95% CI 1.49–4.36]). The number-needed-to-screen by On-Site Screening to detect one additional patient with AED was 13 [95% CI 8–29].</p>
<p>Conclusions </p>
<p>Same-day On-Site Screening by NM UWF imaging increased detection of clinically important diabetic eye disease vs Usual Screening. Integration of NM UWF imaging into routine diabetes clinic visits improved screening adherence and has the potential to prevent vision loss.</p>
Real-World Prevalence of Type 2 Diabetes Remission in a U.S. Insured Population Using a Large Administrative Claims Database
Authors: Sheils Natalie E. , Jarvis Megan S. , Bangerter Lauren R. , ASCHER DAVID , Clark Callahan N. 
Published: December 2022

<p> </p>
<p><strong>Objective.</strong> A 2021 international consensus statement defined type 2 diabetes remission as A1C <6.5% measured at least 3 months after cessation of glucose-lowering therapy. We aimed to investigate whether retrospective claims-based data can assess remission based on this definition, whether three increasingly strict alternative definitions affect the prevalence of remission and characteristics of remission cohorts, and how cohorts with and without sufficient data to assess for remission differ.</p>
<p><strong>Research design and methods.</strong> We used de-identified administrative claims from commercially insured and Medicare Advantage members, enriched with laboratory values, to assess diabetes remission. We used alternative glycemic, temporal, and pharmacologic criteria to assess the sensitivity of remission definitions to changes in claims-based logic. </p>
<p><strong>Results.</strong> Among 524,076 adults with type 2 diabetes, 185,285 (35.4%) had insufficient additional laboratory and/or enrollment data to assess for remission. While more likely to be younger, these individuals had similar initialA1C values and geographical distribution as the 338,791 (64.6%) assessed for remission. Of those assessed for remission, 10,694 (3.2%) met the 2021 consensus statement definition. The proportion of individuals meeting the three alternative definitions ranged from 0.8 to 2.3%. Across all criteria, those meeting the remission definition were more likely to be female, had a lower initially observed A1C, and had a higher prevalence of bariatric surgery.</p>
<p><strong>Conclusion.</strong> This study demonstrates the feasibility of laboratory-value enriched claims-based assessments of type 2 diabetes remission. Establishing stable claims-based markers of remission can enable population assessments of diabetes remission and evaluate the association between remission and clinical outcomes.</p>
Didáctica Kinestésica en la formación presencial del SENA. Caso de estudio: Centro de Comercio y Servicios, Regional Risaralda
Authors: Arenas Jaramillo Adriana María , Martínez de Sánchez Adiela , Sanz Cardona Luz Adriana 
Published: December 2022

Esta investigación se realizó para conocer la percepción de los aprendices del Centro de Comercio y Servicios del SENA, Regional Risaralda, sobre la utilización de actividades didácticas de tipo kinestésico en las sesiones pedagógicas orientadas en los ambientes de formación. El objetivo fue caracterizar actividades que involucren el aprendizaje visual, auditivo y kinestésico, para facilitar la apropiación del conocimiento de las competencias de los programas del Centro de formación. Se utilizó el enfoque de investigación cualitativa exploratoria que apunta a establecer normas de conducta y comportamientos concretos basados en la información obtenida. Se contextualizó con todos los programas de formación ofertados en el Centro de formación durante el segundo y tercer trimestre de 2019. Como conclusión se obtuvo que el efecto de integrar actividades kinestésicas en clase, es percibido por los aprendices como una herramienta de enseñanza interesante y efectiva, aunque su utilización es escasa.
Diseño del sistema de control electrónico para la adquisición de datos de un cultivo vertical de Pleurotus Ostreatus en el Centro de Desarrollo Agroempresarial Chía.
Authors: Zambrano Cortés Nelson Humberto , Garzón Claudia Lorena , Velasco Oskar Fernando , Aragon Stephania 
Published: December 2022

El siguiente articulo refleja los procesos y resultados del trabajo realizado desde el año 2020 para la automatización de un cultivo vertical de Pleurotus Ostreatus para el incremento de cuerpo fructíferos y su posterior análisis para determinar la producción de quitina. El documento presenta el estado del arte de la investigación y el proceso de diseño del sistema de adquisición de datos de temperatura y humedad del cultivo desarrollado.
Antecedentes y Objetivos: El interés por el consumo de hongos comestibles ha incrementado en los últimos años por su vinculación a los alimentos funcionales, debido a que son una fuente de fibra vegetal, minerales, vitaminas y quitina, entre otros. Dentro de los hongos comestibles se encuentra Pleurotus ostreatus, cuya pared celular está compuesta principalmente de quitina, un homopolímero de N – acetilglucosamina el cual ha sido utilizado como materia prima para la fabricación de materiales biomédicos como membranas, esponjas, geles, implantes, nanopartículas y nanofibras. Por lo anterior, se propone el cultivo de P. ostreatus como una alternativa para la obtención de este biopolímero para el desarrollo de materiales biomédicos, direccionando el desarrollo tecnológico y técnicas de control electrónico para la optimización de su producción y siendo esta una oportunidad para innovar sobre dicho proceso.
Para estimular el crecimiento de Pleurotus ostreatus, se diseña un sistema automático que permite el control de las variables de temperatura y humedad y la producción de quitina durante 40 días de cultivo. Nishihara, Watanabe y Asada (2007), describieron la influencia de la temperatura y de la humedad en la expresión génica de la sintasa quitina clase IV durante la formación de cuerpos fructíferos, los cuales le dan la forma al hongo. Sus resultados mostraron que la modulación de la temperatura (25ºC – 15ºC) durante el cultivo incrementó en 1.8 veces la transcripción de la sintasa quitina y así mismo la producción de la misma. De igual forma, los autores proponen estudios sinérgicos entre la variación de la temperatura y la humedad con una proyección de incrementar la concentración de quitina en 1.9 veces.
La automatización del cultivo vertical se desarrolla con el grupo de investigación del SENA CDA - GITAE y su semillero SIPCER en conjunto el grupo de investigación CAPSAB de la Universidad de La Sabana y su semillero INFOSEED. Este trabajo busca el fortalecimiento de las competencias en investigación y desarrollo tecnológico, uniendo esfuerzos y recursos de infraestructura, tecnología y personal para el cumplimiento del objetivo general: Automatizar un cultivo vertical de Pleurotus ostreatus para el incremento de cuerpos fructíferos ricos en quitina.
A xilografia e o livro de horas:
Authors: Caire Silva Regiane Aparecida , CASANOVA Maria da Conceição Lopes Casanova 
Published: December 2022

Resumo
A investigação trata dos primeiros livros impressos, os incunábulos. A tipologia escolhida foi o Livro de Horas, objeto repleto de imagens, destinado à oração em contexto laico. Estudou-se dois livros: o Horae ad usum Romanum (1493) e o Devote ghetidē vanden levē en̄ passie ihū cristi (1498). Objetivou-se comparar a solução gráfica nas duas edições, a repetição da gravura xilográfica relacionada à memória visual e o recurso da cor, com destaque para os fazeres e saberes gráficos do medievo. O resultado mostra que apesar da possibilidade reprodutiva da imagem impressa com a redução de custo, esse recurso foi pouco utilizado nas obras e a estética visual atendeu a públicos diferentes.
Palavras-chave: Livro de Horas, Incunábulo, Xilografia.
Abstract
This research deals with the first printed books, the incunabula. The typology chosen was the Book of Hours, a fully image decorated prayer object used in secular context. Two books were studied: the Horae ad usum Romanum of parchment (1493) and the Devote ghetidē vanden levē en̄ passie ihū cristi of rag paper (1498). The aim was to compare the graphic solution in the two editions, related to visual memory the reiteration of woodcut engraving, the colour use, emphasizing the medieval knowledge and praxis. The result shows that despite the reproductive possibility of the printed image with the cost reduction, this resource was little used in the works and the visual aesthetics served different audiences.
Keywords: Book of Hours, Incunabulum, Woodcut Engraving.
RESULTS OF THE XII INTERNATIONAL SCIENTIFIC CONFERENCE “RUSSIAN SPEECH CULTURE AND TEXT” (TOMSK, MAY 20–21, 2022)
Authors: Болотнова Нина Сергеевна , Болотнов Алексей Владимирович , Савенко Анастасия Сергеевна 
Published: December 2022

Введение. Рассмотрены результаты XII Международной научной конференции, посвященной актуальным проблемам изучения русской речевой культуры и текста в общем научном контексте современной русистики и методики обучения речевой культуре в вузе и школе. Цель – представить обзор докладов, прозвучавших на секциях и пленарных заседаниях конференции «Русская речевая культура и текст» (Томск, 20–21 мая 2022 г.). Материал и методы. Экспертное описание, анализ и обобщение. Результаты и обсуждение. Анализ содержания докладов позволил выявить основные тенденции, характерные для современного речеведения и теории текста, актуальные для дальнейшего развития современной русистики. Намечены перспективы дальнейшего изучения речевой культуры и текста для обучающихся в вузе и школе на основе достижений лингвистической прагматики, когнитивной лингвистики, теории дискурса, медиалингвистики, лингвоперсонологии, стилистики текста, текстоцентрического и аксиологического направлений современной методики преподавания русского языка. Заключение. На конференции определены ключевые темы и проблемы, требующие дальнейшего изучения, намечены планы по укреплению научных связей, позволяющих обмениваться опытом и реализовать имеющие­ся научные разработки в практической научной и образовательной деятельности.
Introduction. The results of the XII International Scientific Conference, dedicated to topical issues of the study of Russian speech culture and text, are considered in the general scientific context of modern Russian studies and methods of teaching speech culture in a university and school practice. The aim is to present a brief review of the reports made at the sections and Plenary sessions of the conference “Russian Speech Culture and Text” (Tomsk, May 20–21, 2022). Material and methods. Expert description, analysis and generalization. Results and discussion. Analysis of the content of the reports made it possible to reveal the main trends characteristic of modern speech science and text theory, which are relevant for the further development of modern Russian studies. The prospects for further study of speech culture and text for university and school students based on the achievements of linguistic pragmatics, cognitive linguistics, discourse theory, media linguistics, linguopersonology, text style, text-centric and axiological directions of modern methods of teaching the Russian language are outlined in the article. Conclusion. The conference identified key topics and issues that require further study, outlined plans to strengthen scientific ties that allow the exchange of experience and the implementation of existing scientific developments in practical scientific and educational activities.
DEICTIC PARTICLES IN THE KHAKASS LANGUAGE
Authors: Чугунекова Алена Николаевна 
Published: December 2022

Представлены результаты исследования дейктических (указательных) частиц в хакасском языке. Дейктические частицы, как и другие указательные слова, выступают в качестве слов, служащих для определения степени удаленности объекта относительно позиции говорящего лица («дейктического центра»). В каждом языке своя (двучленная, трехчленная и более) система определения степени удаленности предмета относительно дейктического центра. В хакасском языке степень удаленности предметов относительно говорящего лица определяется через трехчленную систему: проксимальную («ближе к говорящему лицу»), медиальную («чуть дальше от говорящего лица») и экстремальную («далеко от говорящего лица»). В отличие от дейктических локативных наречий и местоимений, у дейктических частиц наблюдается сближение медиального и экстремального дейксиса: для частицы мына ключевым является ближний дейксис, а для тiгiне и ана – дальний дейксис. Кроме того, данные частицы часто употребляются вместе с соответствующими сериями дейктических наречий, дейктических местоимений и других слов для уточнения идеи указания, общей оценки ситуации, качественно-количественной характеристики объекта и др. Для частицы ана в сочетании со словами, выражающими временной отрезок, характерна анафорическая функция.
This article presents the results of a study of deictic (indicative) particles in the Khakass language. Deictic particles, like other indicative words, act as words that serve to determine the degree of remoteness of an object relative to the position of the speaker (“deictic center”). Each language has its own (binomial, trinomial and more) system for determining the degree of remoteness of the subject relative to the deictic center. In the Khakass language, the degree of remoteness of objects relative to the speaking person is determined through a threeterm system: proximal (“closer to the speaking person”), medial (“a little further from the speaking person”) and extreme (“far from the speaking person”). Unlike deictic locative adverbs and pronouns, deictic particles have a convergence of medial and extreme deixis: for the mna particle, the near deixis is the key, and for tigine and ana, the far deixis is the key. In addition, these particles are often used together with the corresponding series of deictic adverbs, deictic pronouns and other words to clarify the idea of indication, general assessment of the situation, qualitative and quantitative characteristics of the object, etc. An anaphoric function is characteristic of the particle ana in combination with words expressing a spatial and temporal interval.