bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Bright Horizon of Intelligent Targeted-cancer Therapy: Nanoparticles
Against Breast Cancer Stem Cells
Authors: Vaez Ahmad , Abbasi Milad , Shabani Leili , Azizipour Esmat , Shafiee Mostafa , Zare Mohammad Ali , Rahbar Omid , Azari Arezo , Amani Ali Mohammad , Golchin Ali 
Published: October 2022

Abstract:
Breast cancer stem cells (BCSCs) are heterogeneous tumor-initiating cell subgroups of breast
cancers that possess some stem cell markers and are sustained after chemotherapy. Due to BCSCs being
sufficient for tumor relapse, and given that the biological behaviors of BCSCs are so complex, it is critical
to figure out exactly how they work, learn more about their cell biology, and discover biomarkers and
strategies for explicitly targeting and destructing cancer stem cells. In order to accomplish innovative
treatment for breast cancer, it is also essential to target BCSCs. Despite the vast quantities of BCSC target
chemicals, their therapeutic implementation is limited due to off-target behavior and bioavailability issues.
Targeted drug delivery systems based on nanoparticles have advantages for transporting anti-BCSC
materials, especially to targeted locations. Hence, breast cancer therapy using a nanoparticle-based
BCSCs targeting system is a promising strategy. Such targeted drug delivery systems can resolve the
biodistribution obstacles of nanosystems. Throughout this paper, we highlight various strategies for targeting
BCSCs utilizing nano-based systems. In conclusion, issues about the inadequate stability of nanoparticles
and the possibility of loaded drug leakage during delivery systems have yet to be answered.
More fundamental and applied research, and proper methods such as coating or surface modification are
required.
Isoniazid Derivatives as Anti-Tubercular Agents: From Structural Design
to Clinical Investigations
Authors: Rishanlang Nongkhlaw , Ridahunlang Nongkhlaw , Bisht Rohit 
Published: October 2022

Background:
Tuberculosis (TB) is one of the fatal infectious diseases, making it one of the
causes of death in the infectious mortality strata, and it is of prime concern globally. It is spread by a
causative agent called Mycobacterium tuberculosis (Mtb) which gets ingressed within the host cells.
The current clinical interventions have been associated with various limitations, such as a long treatment
regimen (6 months), low lipophilicity of drugs to penetrate the bacterial cell, associated side effects
and emerging incidence of multiple drug-resistant strains. Despite these limitations, Isoniazid
(INH), a first-line agent, remains a drug of choice to date due to its effectiveness. However, INH is
associated with poor penetration into the bacteria cell wall and ultimately leads to the low therapeutic
distribution of drugs into the lungs.

Methods:
Studies have shown that the structural modifications of INH by introducing more lipophilic
moiety could lead to its better penetration into the bacterial cell wall resulting in better anti-TB activities.

Results:
This review updates various studies conducted on INH derivatives as anti-tubercular (Anti-TB)
agents, including in silico and preclinical investigations. In addition, updates on clinical investigations
of novel anti-TB molecules have also been highlighted.

Conclusion:
The article focuses on the structural modification of various INH derivatives reported,
including the in vitro studies and molecular modelling preclinical and clinical investigations of various
INH derivatives.
Effects of GABAergic Agents on Multiple Sclerosis. A Narrative Review of In-vivo Models
Authors: Stamoula Еleni , Ainatzoglou Alexandra , Dardalas Ioannis , Vavilis Theofanis , Stamatellos Vasileios-Periklis , Siafis Spyridon , Psathas Thomas , Boskou Ioanna , Papazisis Georgios 
Published: October 2022

Background:
Multiple sclerosis (MS) is a lifelong deteriorating disease characterized by
multiple heterogeneous symptoms. Being an autoimmune disease of the central nervous system, mainly
affecting the myelin sheath of the nerves ordinarily results in neurological symptoms. GABA has
numerous effects on the immune cells, altering cytokine production, cell migration and proliferation.
Immune cells express GABA receptors making GABA an inflammation modulator. Therefore, GABAergic-
associated agents could provide a compatible add-on therapy for MS patients alleviating their
symptoms and providing better quality years.

Objective:
This review aims to highlight and provide evidence of the potential benefits of a secondary
treatment option in MS patients, aiming to better manage this disease.

Methods:
We conducted a literature search through PubMed, Scopus and Google Scholar for GABA
agonists, antagonists and modulators used in the in vivo model of experimental autoimmune encephalomyelitis
(EAE), taking into consideration certain inclusion and exclusion criteria.

Results:
In vivo studies for GABA-a and GABA-b agonists and modulators showed regulation of the
autoimmune response in EAE mice. Increased preservation of myelinated sensitive fibers and diminished
axonal damage in the CNS was also demonstrated. Further, decreased mononuclear inflammatory
infiltration, pro-inflammatory cytokines reduction and reduced levels of Reactive oxygen species
(ROS) were also reported. Biological results included decreased peak disease severity, duration, clinical
scores and EAE incidence in the treatment groups.

Conclusion:
GABA agonists and modulators efficiently challenged different aspects of disease pathophysiology
in vivo models of EAE. The studies showed a significant relevance of neuroprotection via
modulation of the autoimmune response in EAE rats, indicating that they should be considered proper
therapeutic candidates for clinical use, while also further clinical studies could empower their administration
in clinical practice.
Modernization of a Traditional Siddha Medicine Paccai eruvai into a Novel Nanogel Formulation for the Potent Wound Healing Activity-A Phyto- Pharmaceutical Approach
Authors: Yoganandam Gnanakumar Prakash , Sakthiganapathi Meenachisundaram , Gopal Venkatachalam 
Published: October 2022

Background:Paccai eruvai formulation has been widely used in traditional Siddha practice to treat ulcerous wounds due to the content of potentially active compounds.Objective:The present study aimed to determine the enhancement potency of wound healing of nanogels containing Paccai eruvai in an incision and excision wound models.Methods:Paccai eruvai nanogel was synthesized using the high-energy milling method, and characterization and enhancement of the wound healing potential of Paccai eruvai nanogel were assessed.Results:Reportedly, Paccai eruvai nanogel has been produced successfully and its chemical properties confirmed, and physical properties characterized. Paccai eruvai nanogel showed homogeneity, green color, transparency, and an average size of 19.73 nm. We observed a significant reduction of wound area (p<0.001) in the Paccai eruvai nanogel-treated rats. The percentage of wound contraction on the 16th day was higher than the traditional formulation and nitrofurazone treatment. Notably, a lesser epithelialization period (14.33 days) and higher hydroxyproline content were observed in the 10% Paccai eruvai nanogel rats. We found that 10 % Paccai eruvai nanogel treatment increased tensile strength suggesting a better therapeutic indication.Conclusion:The present findings indicate that Paccai eruvai nanogel significantly contributes wound healing activities with the enhancement of collagen synthesis, wound contraction, and wound tensile strength.
Sustainable Approaches to Boost Yield and Chemical Constituents of Aromatic
and Medicinal Plants by Application of Biostimulants
Authors: Shahrajabian Mohamad Hesam , Sun Wenli 
Published: October 2022

Introduction:
Biostimulants consist of natural ingredients, metabolites of fermentation,
micro-organisms, algae or plant extracts, bacteria, mushrooms, humus substances, amino
acids, biomolecules, etc.

Methods:
In this study, all relevant English-language articles were collected. The literature was
reviewed using the keywords of biostimulant, medicinal plant, aromatic plant, natural products,
and pharmaceutical benefits from Google Scholar, Scopus, and PubMed databases.

Results:
The significant and promoting impact of biostimulants has been reported for different
medicinal and aromatic plants, such as salicylic acid for ajuga, artichoke, ajwain, basil, common
rue, common sage, common thyme, coneflower, coriander, dendrobium, desert Indian wheat,
dragonhead, fennel, fenugreek, feverfew, ginger, groundnut, guava, henna, Iranian soda, lavender,
lemon balm, lemongrass, Malabar spinach; seaweed extract on almond, bird,s eye chili; amino
acids on artemisia, broccoli, chamomile, beneficial bacteria on ashwagandha; humic acid on
black cumin, cannabis, chicory, garlic, gerbera, Hungarian vetch, Moldavian dragonhead, niger
plant; chitosan on dragon fruit, marigold, milk thistle, etc. The suggested mechanisms include the
stimulatory impacts on the activity of enzymes involved in different biosynthetic processes, the
hormone-like activity of biostimulant compounds and the improvement of nutrient uptake of
plants.

Conclusion:
The current manuscript gives many examples of the potential of biostimulants for
medicinal and aromatic plant production. However, further studies are needed to better understand
the effectiveness of different biostimulants and foliar applications in sustainable agriculture.
Knowledge, attitudes, and behaviors of teachers and students toward sustainable development of Pili (Canarium ovatum)
Authors: Funa Aaron Aytona , Gabay Renz Alvin Emberga , Ibardaloza Rosel T. , Limjap Auxencia Alarcon 
Published: October 2022

The urgent call by UNESCO to scale up interventions for the achievement of sustainable development goals motivated this study to assess the level and association of teachers' (n =107) and students' (n = 342) knowledge, attitudes, and behaviors (KAB) regarding education for sustainable development, focusing on Pili (Canarium ovatum). This is a subset of a larger project that involves Pili to contextualize learning and instructional materials. The researchers used a descriptive cross-sectional survey approach and distributed a questionnaire through Google Forms. KAB levels were categorized into poor, moderate, and high using descriptive data analysis and determined their association with one another using Pearson's Chi-square. The findings indicate that, although the associations among the KAB of teachers are distinct from those of the students, they both perceived that possessing favorable knowledge and attitudes are insufficient to generate positive behaviors. Thus, efforts to increase teachers' and students' motivation and involvement and to help them carry out favorable behaviors toward sustainable development are imperative. Given this, identifying the barriers to converting knowledge and attitudes into behaviors and considering the factors that involve the association of KAB in promoting sustainable development, particularly in education, are recommended.
Сlinical portrait of the ambulatory patient with diabetes 2 types
Authors: Kudryashov E. A. , Skibitsky V. V. , Zabolotskikh T. B. , Kudryashova Y. A. 
Published: October 2022

Objective: estimate the clinical status, a metabolic profile and carried-out cardiotropic, hypoglycemic therapy at outpatients with diabetes 2 types in real clinical practice.Material and metods: the outpatient records of 81 patients of both sexes (average age 63.7 + 1.3 years, men 33 (40.7%) with type 2 diabetes mellitus, who are under dispensary observation in one of the polyclinics of Krasnodar, were analyzed. All patients had some clinical (“experience” of diabetes, body mass index) parameters, blood pressure level, laboratory indicators (fasting glycemia, glycated hemoglobin, total cholesterol, high and low density lipoproteins, triglycerides and medication (antihypertensive, lipid-corrective, antithrombotic, hypoglycemic) therapy evaluated.Results: among patients with type 2 diabetes mellitus, women predominated, most of the patients had obesity of the 1st degree. In 27.2% there was no documented concomitant cardiac pathology, the rest had arterial hypertension and coronary artery disease. Antihypertensive therapy included from 2 to 5 drugs, including all patients receiving ACE inhibitors or sartans, which in 2/3 of cases were combined with amlodipine and in equal quantities with diuretics and beta-blockers. Only 8.5% of patients reached the target level of blood pressure. Lipid-lowering therapy in more than 90% of patients included statins in inadequately low doses, which led to the failure to achieve the target level of low-density lipoproteins in 95% of patients. Antithrombotic therapy was taken by more than 90% of patients, but in a quarter of them without documented indications. One in four outpatients with type 2 diabetes mellitus is diagnosed with nonvalvular atrial fibrillation. Only 71.4% of patients received direct oral anticoagulants. Among the means of hypoglycemic therapy, oral drugs (metformin, sulfonylurea derivatives) or their combination with insulin prevailed.Conclusion: in most of the patients examined with type 2 diabetes, the main goals of cardiotropic and hypoglycemic therapy were not achieved. To improve the prognosis and quality of life of such patients, it is necessary to conduct comprehensive adequate treatment based on modern recommendations.
The gender features of the compound body composition in people of middle age with arterial hypertension
Authors: Gorban V. V. , Manto V. S. , Gorban E. V. 
Published: October 2022

Objective: to study the parameters of the compound body composition in middle-aged people with arterial hypertension (AH) to determine the targets for prevention and treatment.Materials and methods: 37 men and 58 women with AH aged 45 to 59 years were examined. Questioning, anthropometry with determination of waist circumference (WC), body mass index (BMI), study of compound body composition by bioimpedancemetry, as well as measurement of blood pressure (BP) were carried out.Results: overweight and obesity were found in 87,3% of middle-aged people with AH. In both sexes, there was a direct correlation of visceral fat (VF) with age, BMI, WC and also with diastolic BP.Conclusion: detection of hypertension, increased BMI, VF is necessary for diagnosing metabolic imbalance in middle-aged people in order to further conduct non-drug and preventive therapy, as well as reduce the development of possible complications.
Factors Associated With Intention and Use of e–Mental Health by Mental Health Counselors in General Practices: Web-Based Survey
Authors: De Veirman Ann E M , Thewissen Viviane , Spruijt Matthijs G , Bolman Catherine A W 
Published: October 2022

Background
Mental health care counselors have a high intention to use e–mental health (EMH), whereas actual use is limited. Facilitating future use requires insight into underlying factors as well as eligibility criteria that mental health care counselors use in their decision to apply EMH.

Objective
The aim of this study was to unfold the intention and underlying reasons for mental health counselors to use EMH and to unveil the criteria they use to estimate patient eligibility for EMH. The theoretical framework was based on the reasoned action approach model, the Unified Theory of Acceptance and Use of Technology, and the Measurement Instrument for Determinants of Innovation model.

Methods
To empirically validate our theoretical model, a web-based survey was conducted among mental health care counselors (n=132). To unveil the eligibility criteria, participants were asked to rank their reasons for considering EMH suitable or unsuitable for a patient.

Results
The mean intention to use EMH was positive (mean 4.04, SD 0.64). The mean use of EMH before the COVID-19 pandemic was 38% (mean 0.38, SD 0.22), and it was 49% (mean 0.49, SD 0.25) during the pandemic. In total, 57% of the patient population was considered eligible for EMH. Usefulness and benefits (β=.440; P<.001), Task perception (β=.306; P=.001), and Accessibility (β=.140; P=.02) explained the intention to use EMH (F3,131=54.151; P<.001; R2=0.559). In turn, intention explained patient eligibility (F1,130=34.716; P<.001; R2=0.211), whereas intention and patient eligibility explained EMH use (F2,129=41.047; P<.001; R2=0.389). Patient eligibility partially mediated the relationship between intention to use EMH and EMH use, with a larger direct effect (c′=0.116; P<.001) than indirect effect (c=0.065, 95% CI 0.035-0.099; P<.001). Mental health counselors assessed patients’ eligibility for EMH mainly through the availability of computers and the internet and patient motivation.

Conclusions
To stimulate the use of EMH, intention and patient eligibility need to be influenced. Intention, in turn, can be enhanced by addressing the perceived usefulness and benefits of EMH, perceived accessibility, and task perception. Access to a computer and patients’ motivation to use EMH are important in facilitating patient eligibility. To cause an impact with EMH in general practice, mental health counselors need to be convinced of the benefits of EMH and transfer this enthusiasm to the patient. It is recommended to involve mental health counselors in the development of EMH to increase the (perceived) added value and use.
A Living Database of HIV Implementation Research (LIVE Project): Protocol for Rapid Living Reviews
Authors: Eshun-Wilson Ingrid , Ford Nathan , Le Tourneau Noelle , Baral Stefan , Schwartz Sheree , Kemp Christopher , Geng Elvin 
Published: October 2022

Background
HIV implementation research evolves rapidly and is often complex and poorly characterized, which makes the synthesis of data on HIV implementation strategies inherently difficult. This is further compromised by prolonged data abstraction processes due to variable interventions, outcomes, and context, and delays in the publication of review findings; this can all result in outdated and irrelevant systematic reviews.

Objective
The LIVE project (A Living Database of HIV Implementation Research) aims to overcome these challenges by applying an implementation science lens to the conduct of rapid living systematic reviews and meta-analyses to inform HIV service delivery priorities and guideline development.

Methods
The LIVE project will generate a series of living systematic reviews exploring implementation strategies for improving HIV cascade outcomes (HIV infection, HIV diagnosis, linkage and retention in HIV care, viral suppression, and mortality). We will search Embase and MEDLINE as well databases specific to review questions for studies conducted after 2004 using predefined search terms to identify studies conducted in any age group or setting, and using implementation strategies that target policy makers, society, health organizations, health workers, and beneficiaries of care and their families. Both randomized controlled trials and observational studies will be included to ensure reviews include pragmatic data. In addition to assessments of methodological quality, features of the implementation strategies, relevance for implementation, and evidence quality will be determined using recognized frameworks. After initial publication, knowledge gaps will be identified, and review questions and search strategies revised to address ongoing critical areas of inquiry. Updated searches will be conducted every 6 months, with subsequent ongoing screening, data abstraction, and revision of meta-analyses.

Results
As of July 2022, five reviews are at various stages of development within the LIVE project. Three systematic reviews are underway and living review processes are in development for two reviews with estimated completion over the next 12 months.

Conclusions
This project and resulting systematic reviews will provide critical insights for HIV service delivery to inform international guideline development.

International Registered Report Identifier (IRRID)
DERR1-10.2196/37070
Mobile Apps for the Management of Gastrointestinal Diseases: Systematic Search and Evaluation Within App Stores
Authors: Messner Eva-Maria , Sturm Niklas , Terhorst Yannik , Sander Lasse B , Schultchen Dana , Portenhauser Alexandra , Schmidbaur Simone , Stach Michael , Klaus Jochen , Baumeister Harald , Walter Benjamin M 
Published: October 2022

Background
Gastrointestinal diseases are associated with substantial cost in health care. In times of the COVID-19 pandemic and further digitalization of gastrointestinal tract health care, mobile health apps could complement routine health care. Many gastrointestinal health care apps are already available in the app stores, but the quality, data protection, and reliability often remain unclear.

Objective
This systematic review aimed to evaluate the quality characteristics as well as the privacy and security measures of mobile health apps for the management of gastrointestinal diseases.

Methods
A web crawler systematically searched for mobile health apps with a focus on gastrointestinal diseases. The identified mobile health apps were evaluated using the Mobile Application Rating Scale (MARS). Furthermore, app characteristics, data protection, and security measures were collected. Classic user star rating was correlated with overall mobile health app quality.

Results
The overall quality of the mobile health apps (N=109) was moderate (mean 2.90, SD 0.52; on a scale ranging from 1 to 5). The quality of the subscales ranged from low (mean 1.89, SD 0.66) to good (mean 4.08, SD 0.57). The security of data transfer was ensured only by 11 (10.1%) mobile health apps. None of the mobile health apps had an evidence base. The user star rating did not correlate with the MARS overall score or with the individual subdimensions of the MARS (all P>.05).

Conclusions
Mobile health apps might have a positive impact on diagnosis, therapy, and patient guidance in gastroenterology in the future. We conclude that, to date, data security and proof of efficacy are not yet given in currently available mobile health apps.
Does multiple gastric aspirate collection increase sensitivity of M. tuberculosis detection in children with pulmonary tuberculosis?
Authors: Galli Luisa , Venturini Elisabetta , Bortone Barbara , Cini Gianmaria , Venanzi Jacopo , Bartolesi Anna Maria , Vaggelli Guendalina , Trapani Sandra , Indolfi Giuseppe , Bianchi Leila , Montagnani Carlotta , Chiappini Elena , Rossolini Gian Maria 
Published: October 2022

Objectives. This study aims to investigate the
sensitivity of microscopy, culture and polymerase chain reaction on
three gastric aspirates (GAs) in the microbiological confirmation of
active pulmonary tuberculosis (TB) and to identify possible changes in
sensitivity derived from the collection of a different number of
aspirates. Methods. Children with clinical and
radiological diagnoses of active pulmonary TB who underwent three GAs
between March 2007 and June 2019 were retrospectively evaluated.
Clinical, radiological, and microbiological data were collected. The
sensitivity of microbiological tests on GAs was calculated. Moreover,
differences in sensitivity according to age and radiological pattern
were investigated. Results. Overall, 156 children with
active pulmonary TB were enrolled with a median age of 51.5 (IQR:
25.2–113.2) months. Microbiological investigations on the first GA
showed a sensitivity of 34% (95%CI 26.7, 42), the cumulative
sensitivity of first and second GAs was 40.4% (95%CI 32.7, 48.5) and
of the three GAs was 47.4% (95%CI 39.8, 55.2). The collection of three
GAs leads to an overall increase in sensitivity of the first GA by
13.4% (95%CI 2.8, 24.1%; p=0.014). Moreover, the increase in
sensitivity was significantly higher in children < 4 years of
age and in those with uncomplicated TB (p=0.008). Conclusions. Performing a higher number of GAs increases the sensitivity of
microbiological confirmation of active pulmonary TB, particularly in
children ≤ 4 years and with an uncomplicated radiological pattern.