bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physical Activity and Health-related Quality of Life from Diagnosis to One Year After Radical Cystectomy in Patients with Bladder Cancer: A Longitudinal Cohort Study
Authors: Rammant Elke , Van Hecke Ann , Van Cauwenberg Jelle , Decaestecker Karel , Poppe Lindsay , Russel Beth , Bultijnck Renée , Albersen Maarten , Verhaeghe Sofie , Van Hemelrijck Mieke , Colman Roos , Deforche Benedicte , Fonteyne Valérie 
Published: August 2022

BACKGROUND: Emerging evidence shows a positive impact of physical activity (PA) on health-related quality of life (HRQoL) in cancer patients. However, longitudinal evidence on PA and HRQoL in patients with bladder cancer (BC) undergoing radical cystectomy (RC) is lacking. OBJECTIVES: To investigate PA levels, HRQoL outcomes and their relationship from diagnosis to one year after RC in BC patients. METHODS: A longitudinal cohort study in 90 BC patients was conducted at Ghent and Leuven University Hospitals between April 2017 and December 2020. The Godin Leisure-Time Exercise Questionnaire (GLTEQ) and the EORTC QLQ-C30 and BLM30 were used to measure PA and HRQoL, respectively, before RC, one, three, six and twelve months after RC. Linear mixed models were used for statistical analyses. RESULTS: The majority was physically inactive before RC (58%), at month one (79%), three (53%), six (61%) and twelve (64%). Among (moderately) active patients, light-intensity activities (mainly walking) were important contributors to the total amount of PA. Clinically important and low HRQoL outcomes in different domains were identified with lowest scores at diagnosis and one month after RC. Active patients before RC have better physical functioning (mean difference (MD) -22.7, standard error (SE) 8.7, p = 0.011), global health status (MD -15.9, SE 6.9, p = 0.023) and fatigue (MD 19.9, SE 9.5, p = 0.038) one month after RC, compared to inactive patients. Active patients at month have better physical functioning (MD -16.2, SE 6.9, p = 0.023) and sexual functioning (MD -16.8, SE 5.4, p = 0.003; MD -13.5, SE 5.5, p = 0.017) at month six and twelve, respectively, compared to inactive patients. CONCLUSIONS: Higher PA levels are associated with better HRQoL outcomes for BC patients undergoing RC. The data suggests that PA interventions could be an asset to improve BC patients’ HRQoL, but should be tested in future trials.
Orthopedic Interventions for Foot Deformities in Non-Ambulant People with Duchenne Muscular Dystrophy: A Retrospective Study on Indications, Post-Operative and Long-Term Outcomes
Authors: Houwen-van Opstal Saskia L.S. , Timmer Amity C. , Ten Ham A.M. , Hosman Allard J.F. , Willemsen Michel A.A.P. , de Groot Imelda J.M. 
Published: August 2022

Background: Progressive equinovarus deformities are common in people with Duchenne Muscular Dystrophy (DMD); they may provoke pain, pressure spots, cause problems with wearing footwear, and may lead to an unstable sitting position. Objective: Explore indications and compare complications and long-term outcomes after soft tissue and osseous interventions in people with DMD. Methods: Retrospective, monocenter, longitudinal study. Data on indications, equinus and varus deformity before and after surgery, wound healing problems, ‘pain’, edema, and long-term outcomes were collected from medical files. Soft tissue interventions were compared with osseous interventions. Results: From a series of 18 patients, data on 32 surgical interventions and 169 follow-up visits were analyzed. ‘Footrest placement’ was the most frequent surgical indication, followed by pain. Osseous interventions were performed in older patients with rigid deformities. Directly after surgery remaining deformities were reported after soft tissue interventions (18 %), no remaining deformities were reported after osseous interventions. Pain and edema were frequently present, especially after osseous surgery. Longitudinal follow-up showed that surgical interventions could lead to a neutral foot for a for more than 3 years on average years. Relapses of foot deformity occurred, especially the recurrence of varus deformity after osseous interventions. Conclusions: Surgical interventions can successfully lead to a neutral foot position for for more than 3 years on average. Soft tissue interventions appear to be superior to osseous corrections, considering the varus recurrence period and complications, and may be considered when feet are still (partly) correctable. Pain management and edema prevention should be anticipated before surgery. Future research on patient reported outcomes as well as evaluating the outcome of the initial indication is needed to further identify benefits.
Budidaya Sayur Hidroponik Sistem Sumbu/Wick Sebagai Upaya Kemandirian Pangan Pasca Pandemi
Authors: Mukhibin M Infarul 
Published: August 2022

Berdasarkan analisis situasi diketahui bahwa sebagian besar masyarakat Desa kalang semanding bermata pencarian disektor pertanian dan produk yang dihasilkan berupa kacang kedelai, padi, dan bawang merah tetapi untuk jenis tanaman sayuran produksinya sangat rendah dan bahkan tidak dibudidayakan oleh penduduk setempat. Padahal, potensi pengembangan pertanian pada skala rumah tangga atau perkarangan cukup besar.
Tujuan program ini adalah (1) Untuk memberdayakan masyarakat Desa Kalang semanding dengan memberikan pelatihan budidaya tanaman sayuran dengan hidroponik sistem wick / Sumbu (2) meningkatkan pengetahuan masyarakat tentang teknik hidroponik. Konsep hidroponikmerupakan budidaya menanam dengan memanfaatkan serabu kayu,serat kelapa dan sekam. Metode yang digunakan dalam program ini adalah sosialisasi program, pelatihan dan praktik pembuatan hidroponik dan pendampingan serta evaluasi kegiatan. Berdasarkan hasil program yang dijalankan dapat disimpulkan bahwa budidaya tanaman sayuran hidroponik sistem wick / Sumbu telah berhasil memberdayakan ibu-ibu rumah tangga masyarakat Desa Kalang Semanding dalam meningkatkan minat untuk memanfaatkan perkarangan sebagai lahan penanaman sayuran. Masyarakat memberikan respon yang sangat positif terhadap kegiatan yang dilakukan dan menilai kegiatan ini sangat bermanfaat
Identifikasi Prioritas Pemeliharaan Jalan Provinsi Menggunakan Metode Simple Additive Weighting
Authors: Haryadi Octadino , Rangga Bakti Imam 
Published: August 2022

Jalan merupakan media untuk transportasi darat yang menghubungkan daerah satu dengan daerah lainnya. Jalan merupakan infastruktur yang bersifat umum dimana semua kalangan dapat menggunakannya dalam keseharian. Kondisi jalan merupakan faktor yang mempengaruhi kenyamanan pengguna jalan dan juga merupakan faktor yang menjadi alasan banyaknya kecekalaan lalu lintas. Banyaknya masalah kondisi jalan menjadi suatu kendala yang tidak dapat dipungkiri dari setiap wilayah di Indonesia begitu juga di Provinsi Riau. Sistem penunjang keputusan adalah sebuah sistem untuk mencari penunjang keputusan ,keputusan diambil menggunakan sistem yang dirancang berdasarkan kebutuhan pemakaian, dalam membantu mengambil suatu keputusan, keputusan dirancang berdasarkan kriteria dan alternatife yang sudah ditentukan sebelumnya dan memiliki sistem yang sudah terstruktur dan terprogram dalam bentuk pembobotan yang akan diakumulasi dan dinormalisasikan dan menghasilkan perangkingan. Penelitian ini menggunakan metode Simple Additive Weighting biasa disebut sebagai metode yang menggunakan bentuk penjumlah terbobot. Bentuk.dari.metode.yaitu menghitung jumlah nilai bobot dari langkah-langkah kerja pada alternatif yang ada dari semua atribut. Untuk menjelaskan tentang penyelesaian metode penulis menggunakan data jalan provinsi. Banyak data jalan yang akan dijadikan alternatif pada penelitian ini sebanyak 20 data jalan provinsi. Dengan hasil priotias perbaikan jalan provinsi menghasilkan 11 jalan yang menjadi prioritas dengan nilai akhir di atas 50
Low-intensity pulsed ultrasound promotes periodontal regeneration in a beagle model of furcation involvement
Authors: Wang Yue , Xiao Qingyue , Zhong Wenjie , Zhang Chuangwei , Yin Yuanyuan , Gao Xiang , Song Jinlin 
Published: August 2022

Objective: To evaluate the regeneration potential of periodontitis tissue treated by low-intensity pulsed ultrasound (LIPUS) combined with the guided tissue regeneration (GTR) technique in a beagle model of furcation involvement (FI).Background: Achieving predictable regeneration remains a clinical challenge for periodontitis tissue due to the compromised regenerative potential caused by chronic inflammation stimulation. LIPUS, an FDA-approved therapy for long bone fracture and non-unions, has been demonstrated effective in the in vitro attenuation of inflammation-induced dysfunction of periodontal ligament stem cells (PDLSCs), the key cells contributing to periodontal regeneration. However, the in vivo effect of LIPUS on periodontitis tissue is rarely reported.Methods: A beagle model of FI was established, and the experimental teeth were randomly assigned into three groups: control group, GTR group, and GTR+LIPUS group. Radiographic examinations were performed, and clinical periodontal parameters were recorded to reflect the periodontal condition of different groups. Histological analyses using H&amp;E and Masson’s staining were conducted to evaluate the periodontal tissue regeneration.Results: LIPUS could enhance new periodontal bone formation and bone matrix maturity in FI after GTR treatment. Moreover, clinical assessment and histomorphometric analyses revealed less inflammatory infiltration and superior vascularization within bone grafts in the LIPUS treatment group, indicating the anti-inflammatory and pro-angiogenic effects of LIPUS in FI.Conclusion: Our investigation on a large animal model demonstrated that LIPUS is a promising adjunctive approach for the regeneration of periodontitis tissue, paving a new avenue for LIPUS application in the field of periodontal regenerative medicine.
Transcriptional comparison of adult human primary Retinal Pigment Epithelium, human pluripotent stem cell-derived Retinal Pigment Epithelium, and ARPE19 cells
Authors: Markert Elke K. , Klein Holger , Viollet Coralie , Rust Werner , Strobel Benjamin , Kauschke Stefan G. , Makovoz Bar , Neubauer Heike , Bakker Remko A. , Blenkinsop Timothy A. 
Published: August 2022

The therapeutic potential of pluripotent stem cells is great as they promise to usher in a new era of medicine where cells or organs may be prescribed to replace dysfunctional tissue. At the forefront are efforts in the eye to develop this technology as it lends itself to in vivo monitoring and sophisticated non-invasive imaging modalities. In the retina, retinal pigment epithelium (RPE) is the most promising replacement cell as it has a single layer, is relatively simple to transplant, and is associated with several eye diseases. However, after transplantation, the cells may transform and cause complications. This transformation may be partially due to incomplete maturation. With the goal of learning how to mature RPE, we compared induced pluripotent stem cell-derived RPE (iPSC-RPE) cells with adult human primary RPE (ahRPE) cells and the immortalized human ARPE-19 line. We cultured ARPE-19, iPSC-RPE, and ahRPE cells for one month, and evaluated morphology, RPE marker staining, and transepithelial electrical resistance (TEER) as quality control indicators. We then isolated RNA for bulk RNA-sequencing and DNA for genotyping. We genotyped ahRPE lines for the top age-related macular degeneration (AMD) and proliferative vitreoretinopathy (PVR) risk allele polymorphisms. Transcriptome data verified that both adult and iPSC-RPE exhibit similar RPE gene expression signatures, significantly higher than ARPE-19. In addition, in iPSC-RPE, genes relating to stem cell maintenance, retina development, and muscle contraction were significantly upregulated compared to ahRPE. We compared ahRPE to iPSC-RPE in a model of epithelial-mesenchymal transition (EMT) and observed an increased sensitivity of iPSC-RPE to producing contractile aggregates in vitro which resembles incident reports upon transplantation. P38 inhibition was capable of inhibiting iPSC-RPE–derived aggregates. In summary, we find that the transcriptomic signature of iPSC-RPE conveys an immature RPE state which may be ameliorated by targeting “immature” gene regulatory networks.
Simple immunosensor for ultrasensitive electrochemical determination of biomarker of the bone metabolism in human serum
Authors: Chang Qiang , Huang Jie , He Liming , Xi Fengna 
Published: August 2022

Ultrasensitive and selective determination of biomarkers of the bone metabolism in serum is crucial for early screening, timely treatment, and monitoring of the curative effect of osteoporosis, which is a silent disease with serious health threats. Immunoassay with a simple sensing interface and ultrahigh sensitivity is highly desirable. Herein, a simple electrochemical immunosensor is demonstrated based on gold nanoparticles (AuNPs) electrodeposited on chitosan-reduced graphene oxide (CS-G) composite modified electrode, which can achieve sensitive determination of the important biomarker of bone metabolism, bone gamma-carboxyglutamate protein (BGP). To overcome the agglomeration of graphene and introduce a biocompatible matrix with functional amino groups, CS-G is prepared and modified on the supporting glassy carbon electrode (GCE). Then, AuNPs are electrodeposited on CS-G through their interaction between amine groups of CS. The immobilized AuNPs provide numerous binding sites to immobilize anti-BGP antibodies (AbBGP). The specific recognition between BGP and AbBGP results in a reduction in the mass transfer of the electrochemical probe (Fe(CN)63-/4-) in solution, leading to a reduced electrochemical signal. Based on this mechanism, fast and ultrasensitive electrochemical detection of BGP is achieved when the concentration of BGP ranges from 100 ag ml−1 to 10 μg mL−1 with a limit of detection (LOD) of 20 ag ml−1 (S/N = 3). The determination of BGP in human serum is also realized with high reliability.
Global epidemiology, genetic environment, risk factors and therapeutic prospects of mcr genes: A current and emerging update
Authors: Mmatli Masego , Mbelle Nontombi Marylucy , Osei Sekyere John 
Published: August 2022

BackgroundMobile colistin resistance (mcr) genes modify Lipid A molecules of the lipopolysaccharide, changing the overall charge of the outer membrane.Results and discussionTen mcr genes have been described to date within eleven Enterobacteriaceae species, with Escherichia coli, Klebsiella pneumoniae, and Salmonella species being the most predominant. They are present worldwide in 72 countries, with animal specimens currently having the highest incidence, due to the use of colistin in poultry for promoting growth and treating intestinal infections. The wide dissemination of mcr from food animals to meat, manure, the environment, and wastewater samples has increased the risk of transmission to humans via foodborne and vector-borne routes. The stability and spread of mcr genes were mediated by mobile genetic elements such as the IncHI2 conjugative plasmid, which is associated with multiple mcr genes and other antibiotic resistance genes. The cost of acquiring mcr is reduced by compensatory adaptation mechanisms. MCR proteins are well conserved structurally and via enzymatic action. Thus, therapeutics found effective against MCR-1 should be tested against the remaining MCR proteins.ConclusionThe dissemination of mcr genes into the clinical setting, is threatening public health by limiting therapeutics options available. Combination therapies are a promising option for managing and treating colistin-resistant Enterobacteriaceae infections whilst reducing the toxic effects of colistin.
Barriers to hydroxyurea use from the perspectives of providers, individuals with sickle cell disease, and families: Report from a U.S. regional collaborative
Authors: Treadwell Marsha J. , Du Lisa , Bhasin Neha , Marsh Anne M. , Wun Theodore , Bender M. A. , Wong Trisha E. , Crook Nicole , Chung Jong H. , Norman Shannon , Camilo Nicolas , Cavazos Judith , Nugent Diane 
Published: August 2022

Sickle cell disease (SCD) is an inherited blood disorder that affects about 100,000 people in the U.S., primarily Blacks/African-Americans. A multitude of complications negatively impacts quality of life. Hydroxyurea has been FDA approved since 1998 as a disease-modifying therapy for SCD, but is underutilized. Negative and uninformed perceptions of hydroxyurea and barriers to its use hinder adherence and promotion of the medication. As the largest real-world study to date that assessed hydroxyurea use for children and adults with SCD, we gathered and analyzed perspectives of providers, individuals with SCD, and families. Participants provided information about socio-demographics, hospital and emergency admissions for pain, number of severe pain episodes interfering with daily activities, medication adherence, and barriers to hydroxyurea. Providers reported on indications for hydroxyurea, reasons not prescribed, and current laboratory values. We found that hydroxyurea use was reported in over half of eligible patients from this large geographic region in the U.S., representing a range of sickle cell specialty clinical settings and practices. Provider and patient/caregiver reports about hydroxyurea use were consistent with one another; adults 26 years and older were least likely to be on hydroxyurea; and the likelihood of being on hydroxyurea decreased with one or more barriers. Using the intentional and unintentional medication nonadherence framework, we found that, even for patients on hydroxyurea, challenges to taking the medicine at the right time and forgetting were crucial unintentional barriers to adherence. Intentional barriers such as worry about side effects and “tried and it did not work” were important barriers for young adults and adults. For providers, diagnoses other than HgbSS or HgbS-β0 thalassemia were associated with lower odds of prescribing, consistent with evidence-based guidelines. Our results support strengthening provider understanding and confidence in implementing existing SCD guidelines, and the importance of shared decision making. Our findings can assist providers in understanding choices and decisions of families; guide individualized clinical discussions regarding hydroxyurea therapy; and help with developing tailored interventions to address barriers. Addressing barriers to hydroxyurea use can inform strategies to minimize similar barriers in the use of emerging and combination therapies for SCD.
Liver transcriptomics reveals features of the host response in a mouse model of dengue virus infection
Authors: Zheng Wenjiang , Yan Qian , Li Zonghui , Wang Xianyang , Wu Peng , Liao Feng , Lao Zizhao , Jiang Yong , Liu Xiaohong , Zhan Shaofeng , Li Geng 
Published: August 2022

BackgroundDengue virus (DENV) infection induces various clinical manifestations and even causes organ injuries, leading to severe dengue haemorrhagic fever and dengue shock syndrome. Hepatic dysfunction was identified as a risk predictor of progression to severe disease during the febrile phase of dengue. However, the underlying mechanisms of hepatic injury remain unclear.MethodsA model of dengue disease was established in IFNAR−/− C57BL/6 mice by challenge with DENV-2. Body weight, symptoms, haematological parameters and liver pathological observations in mice were used to determine the effects of DENV infection. Liver transcriptome sequencing was performed to evaluate the features of the host response in IFNAR−/− mice challenged with DENV. Functional enrichment analysis and analysis of significantly differentially expressed genes (DEGs) were used to determine the critical molecular mechanism of hepatic injury.ResultsWe observed haemoconcentration, leukopenia and liver pathologies in mice, consistent with findings in clinical dengue patients. Some differences in gene expression and biological processes were identified in this study. Transcriptional patterns in the liver indicated that antiviral responses to DENV and tissue damage via abnormal expression of proinflammatory cytokines were induced. Further analysis showed that the upregulated DEGs were significantly enriched in the leukocyte transendothelial migration, complement and coagulation cascades, and cytokine-cytokine receptor interactions signalling pathways, which are considered to be closely associated with the pathogenic mechanism of dengue. IL6, IL 10, ICAM-1, VCAM-1, MMP9 and NLRP3 were identified as biomarkers of progression to severe disease.ConclusionsThe interactions of these cytokines, which activate inflammatory signalling, may lead to organ injury and haemoconcentration and even to vascular leakage in tissues, including the mouse liver. Our study identifies candidate host targets that could be used for further functional verification.
Etanercept/celecoxib on improving MRI inflammation of active ankylosing spondylitis: A multicenter, open-label, randomized clinical trial
Authors: Tu Liudan , Zhao Minjing , Wang Xiaohong , Kong Qingcong , Chen Zena , Wei Qiujing , Li Qiuxia , Yu Qinghong , Ye Zhizhong , Cao Shuangyan , Lin Zhimin , Liao Zetao , Lv Qing , Qi Jun , Jin Ou , Pan Yunfeng , Gu Jieruo 
Published: August 2022

ObjectiveTo investigate the efficacy and safety of clinical, magnetic resonance imaging (MRI) changes in active ankylosing spondylitis (AS) patients with etanercept and celecoxib alone/combined treatment.MethodsA randomized controlled trial was conducted in three medical centers in China. Adult AS patients with BASDAI ≥4 or ASDAS ≥2.1, CRP &gt;6 mg/L, or ESR 28 mm/1st hour were randomly assigned (1:1:1 ratio) to celecoxib 200 mg bid or etanercept 50 mg qw or combined therapy for 52 weeks. The primary outcomes were SPARCC change of the sacroiliac joint (SIJ) and spine and the proportion of patients achieving ASAS20 response at 52 weeks.ResultsBetween September 2014 and January 2016, we randomly assigned 150 patients (mean age, 32.4 years; mean disease duration, 109 months), and 133 (88.6%) completed the study. SPARCC inflammation scores of the SIJ and spine decreased in the three groups, and significant differences were found between the combined group and the celecoxib group [between-group difference: −6.33, 95% CI (−10.56, −2.10) for SIJ; −9.53, 95% CI (−13.73, −5.33) for spine] and between the etanercept group and the celecoxib group [between-group difference: −5.02, 95% CI (−9.29, −0.76) for SIJ; −5.80, 95% CI (−10.04, −1.57) for spine]. The ASAS20 response rates were 44%, 58%, and 84% in the celecoxib, etanercept, and combined groups, respectively, and a significant difference was only found between the combined and the celecoxib groups.ConclusionEtanercept with or without celecoxib decreases inflammation detected by MRI at 1 year compared to celecoxib alone in active AS patients. The combination of etanercept and celecoxib was superior to celecoxib alone for the primary clinical response.Clinical Trial RegistrationClinicalTrials.gov, identifier NCT01934933.