bluuebunny/crossref_metadata_2025_split huggingface dataset:
16S rRNA gene‐based identification and plant growth‐promoting potential of cultivable endophytic bacteria
Authors: Raimi Adekunle , Adeleke Rasheed 
Published: October 2022

AbstractEndophytic bacteria play essential roles in plant growth and fitness through nitrogen fixation, nutrient solubilization, and phytohormone production. However, the plant growth‐promoting (PGP) potential of endophytic microorganisms inhabiting vegetables is underexplored. Thus, this study investigated the endophytic bacteria of vegetable crops for their PGP properties using the culture‐dependent technique. Recovered isolates were identified by sequencing the partial 16S rRNA gene using Sanger sequencing. The isolated endophytic bacteria belonged to 15 genera and 39 different species.Bacillus, Enterobacter, Pantoea, andPseudomonaswere the predominant genera and had high phosphate solubilization and siderophore production potential. The isolates differentially produced indole‐3‐acetic acid and ammonium in the range 0.14–44.48 μg mL–1and 0.77–93.24 mg L–1, respectively.Pseudomonas azotoformans, Stenotrophomonas maltophilia, andSerratia liquefaciensshowed a high level of siderophore production, which was not significantly (p > 0.05) different across the plant and organ types. More than 21% of endophytes could produce gelatinase, protease, amylase, and cellulase, contributing to their diverse ecosystem functions. Bacterial density and species composition varied across the vegetable species, organs, and farm locations but were higher in the stems compared with other organs. However, the vegetable organ had no significant (p > 0.05) influence on the bacterial population density, except for the interaction of plant species and organs. Overall, this study provides basic knowledge about the cultivable endophytic bacterial community of vegetables and reveals diverse endophytic bacteria useful in bioinoculant formulations for improving crop productivity.
Identification of the reaction network for the synthesis of adipic acid using machine learning coupling with target factor analysis
Authors: Wei Hui‐Long , Ouyang Bo , Zhu Li‐Tao , Su Yuan‐Hai , Luo Zheng‐Hong 
Published: October 2022

AbstractThis study investigated the reaction network of the oxidation of cyclohexanone with nitric acid through machine learning (ML) model coupling with target factor analysis (TFA). Experiments for the synthesis of adipic acid (AA) were carefully designed and carried out in a microreactor system. An artificial neural network (ANN) model was applied and optimized by training on experimental data. To assess the established ANN model, a comparison between the predicted concentrations of the products and those calculated with the power law kinetic model was implemented. TFA was then performed on both the experimental data and the data simulated by ANN for identifying the candidate reactions and finding out the temperature boundaries. Based on the identification results of the reaction network, the kinetic characteristics of this oxidation process under various operational conditions were further researched through the incremental approach and simultaneous approach.
Designing polysulfides adsorption‐conversion on g‐C3N4‐based separator via doping heteroatoms boron and phosphorus toward high‐performance lithium‐sulfur batteries
Authors: Sun Yajie , Huang Wenzhi , Li Junhao , Pan Jiajie , Nan Haoxiong , Dong Huafeng , Shi Kaixiang , Liu Quanbing 
Published: October 2022

AbstractThe notorious “shuttle effect” of lithium polysulfides (LiPSs) has suppressed the large‐scale commercial application of lithium‐sulfur batteries (LSBs). Also, the intrinsic advantages including inhibiting the shuttling‐circulation and promoting the conversion of LiPSs, on the separator are deemed as stimulating blocks on the exploitation of LSBs. Herein, guided by theoretical calculations, we have designed doping‐heteroatoms boron (B) and phosphorus (P) on graphitic carbon nitride (g‐C3N4), to suppress the “shuttle effect” of LiPSs and improve the battery cycling performance. Theoretical calculations show strong chemical bonding between Li atom and N substance of g‐C3N4, and corroborates synergistic mechanism of electron transfer and van der Waals interaction. The high adsorption energies with doping B/P atoms enable LiPSs adsorption on the separators inhibiting shuttle loss. As expected, the experiment data also demonstrate that superior electrochemical performance are achieved by utilizing B‐doped g‐C3N4 (BCN) separator. Incorporating the B‐doped g–C3N4 (BCN) separator in LSBs delivered an initial specific capacity of 1050 mAh g−1 at 1 C and 920 mAh g−1 at 2 C. Notably, it achieved a decay rate of 0.08% and 0.1% at 1 and 2 C, respectively, after 500 cycles. This study furthers our understanding of B/P doping separator enhancement and restraining LiPSs shuttling loss for an improved LSBs performance.
Retrospective evaluation of clinical and molecular data of 148 cases of esophageal atresia
Authors: Ranza Emmanuelle , Le Gouez Morgane , Guimier Anne , Dunlop Naziha Khen , Beaudoin Sylvie , Malan Valérie , Michot Caroline , Baujat Geneviève , Rio Marlène , Cormier‐Daire Valérie , Abadie Véronique , Sarnacki Sabine , Delacourt Christophe , Lyonnet Stanislas , Attié‐Bitach Tania , Pingault Véronique , Rousseau Véronique , Amiel Jeanne 
Published: October 2022

AbstractDevelopmental abnormalities provide a unique opportunity to seek for the molecular mechanisms underlying human organogenesis. Esophageal development remains incompletely understood and elucidating causes for esophageal atresia (EA) in humans would contribute to achieve a better comprehension. Prenatal detection, syndromic classification, molecular diagnosis, and prognostic factors in EA are challenging. Some syndromes have been described to frequently include EA, such as CHARGE, EFTUD2‐mandibulofacial dysostosis, Feingold syndrome, trisomy 18, and Fanconi anemia. However, no molecular diagnosis is made in most cases, including frequent associations, such as Vertebral‐Anal‐Cardiac‐Tracheo‐Esophageal‐Renal‐Limb defects (VACTERL). This study evaluates the clinical and genetic test results of 139 neonates and 9 fetuses followed‐up at the Necker‐Enfants Malades Hospital over a 10‐years period. Overall, 52 cases were isolated EA (35%), and 96 were associated with other anomalies (65%). The latter group is divided into three subgroups: EA with a known genomic cause (9/148, 6%); EA with Vertebral‐Anal‐Cardiac‐Tracheo‐Esophageal‐Renal‐Limb defects (VACTERL) or VACTERL/Oculo‐Auriculo‐Vertebral Dysplasia (VACTERL/OAV) (22/148, 14%); EA with associated malformations including congenital heart defects, duodenal atresia, and diaphragmatic hernia without known associations or syndromes yet described (65/148, 44%). Altogether, the molecular diagnostic rate remains very low and may underlie frequent non‐Mendelian genetic models.
Anticipatory banking of samples enables diagnosis of adenylosuccinase deficiency following molecular autopsy in an infant with vacuolating leukoencephalopathy
Authors: Sitaram Spatikha , Banka Hetalika C. , Vassallo Grace , Pavaine Julija , Fairclough Adele , Wright Ronnie , Fairbanks Lynette , Bierau Jörgen , Bowden Lydia , Schwahn Bernd , Horman Alistair , Banka Siddharth 
Published: October 2022

AbstractAdenylosuccinase deficiency is a rare inborn error of metabolism. We present a newborn who died at 52 days of age with clinical features suggestive of severe epileptic encephalopathy and leukodystrophy of unknown cause. Post‐mortem examination showed an unusual vacuolar appearance of the brain. A molecular autopsy performed via singleton clinical exome analysis revealed a known pathogenic and a variant of uncertain significance in ADSL that encodes adenylosuccinase. Tests on previously stored plasma samples showed elevated succinyladenosine and succinylaminoimidazole carboxamide riboside levels. Adenylosuccinase activity in stored fibroblasts was only ~5% of control confirming the diagnosis of adenylosuccinase deficiency in the child. The parents opted for a chorionic villus biopsy in a subsequent pregnancy and had a child unaffected by adenylosuccinase deficiency. This report adds vacuolating leukodystrophy as a novel feature of adenylosuccinase deficiency and shows the power of biochemical investigations directed by genomic studies to achieve accurate diagnosis. Importantly, this case demonstrates the importance of anticipatory banking of biological samples for reverse biochemical phenotyping in individuals with undiagnosed disorders who may not survive.
Muscle spasms as presenting feature of Nivelon‐Nivelon‐Mabile syndrome
Authors: Saini Neelam , Das Bhowmik Aneek , Yareeda Sireesha , Venkatapuram Vijayasree , Jabeen Shaik Afshan , Tallapaka Karthik , Dalal Ashwin , Aggarwal Shagun 
Published: October 2022

AbstractHedgehog acyltransferase gene (HHAT)‐associated Nivelon‐Nivelon‐Mabile syndrome (NNMS) is a rare genetic disorder of multiple system involvement with microcephaly, central nervous system malformations, skeletal dysplasia, and 46,XY sex reversal. Other variable and inconsistent features reported in this disorder are muscle spasms, facial dysmorphism, prenatal onset growth restriction, microphthalmia, and holoprosencephaly. This is the sixth postnatal reported patient with biallelic variants in HHAT gene, who presented with microcephaly, short stature, muscle hypertrophy, muscle spasms, and facial dysmorphism. The most prominent and presenting finding in this patient were muscle hypertrophy and muscle spasms which had a clinical response to phenytoin and acetazolamide treatment. Our report emphasizes the phenotypic variability of NNMS and further reiterates muscle spasms as an important clinical manifestation of this extremely rare condition.
A multicenter study to evaluate pain characteristics in osteogenesis imperfecta
Authors: Rodriguez Celin Mercedes , Kruger Karen M. , Caudill Angela , Murali Chaya N. , Nagamani Sandesh C. S. ,   Members of the Brittle Bone Disorders Consortium (BBDC), Smith Peter A. , Harris Gerald F. 
Published: October 2022

AbstractThe objective was to describe pain characteristics and treatments used in individuals with varying severity of osteogenesis imperfecta (OI) and investigate pain‐associated variables. This work was derived from a multicenter, longitudinal, observational, natural history study of OI conducted at 12 clinical sites of the NIH Rare Diseases Clinical Research Network's Brittle Bone Disorders Consortium. Children and adults with a clinical, biochemical, or molecular diagnosis of OI were enrolled in the study. We did a cross‐sectional analysis of chronic pain prevalence, characteristics, and treatments used for pain relief and longitudinal analysis to find the predictors of chronic pain. We included 861 individuals with OI, in 41.8% chronic pain was present, with similar frequency across OI types. Back pain was the most frequent location. Nonsteroidal anti‐inflammatory drugs followed by bisphosphonates were the most common treatment used. Participants with chronic pain missed more days from school or work/year and performed worse in all mobility metrics than participants without chronic pain. The variables more significantly associated with chronic pain were age, sex, positive history of rodding surgery, scoliosis, other medical problems, assistive devices, lower standardized height, and higher body mass index. The predictors of chronic pain for all OI types were age, use of a wheelchair, and the number of fractures/year. Chronic pain is prevalent in OI across all OI types, affects mobility, and interferes with participation. Multiple covariates were associated with chronic pain.
Copper‐Catalyzed Simultaneous Installation of Boryl and Carbonyl Functionalities to Unsaturated Hydrocarbons
Authors: Fujihara Tetsuaki 
Published: October 2022

AbstractCu‐catalyzed borylative functionalization of unsaturated hydrocarbons is an effective method for synthesizing various organoboron compounds under mild reaction conditions. In particular, installing carbonyl functionalities such as acyl, formyl, and carboxy groups is interesting because of the essential role of these functionalities in organic synthesis. This review focuses on the installation of boryl and carbonyl functionalities in unsaturated hydrocarbons using Cu catalysts. First, formylation and acylation reactions using formate esters, acyl halides, or acid anhydrides are addressed. Second, the simultaneous incorporation of a boryl group and a carboxy group using carbon dioxide as the electrophile is summarized. Third, the borocyanation of unsaturated hydrocarbons using electrophilic cyanation reagents is described.
Design and Synthesis of Amino Acid Derivatives via Diethyl Acetadimalonate and Late‐Stage Modification by Chemical Methods
Authors: Kotha Sambasivarao , Gaikwad Vidyasagar , Chaurasia Usha Nandan 
Published: October 2022

AbstractWe report various unusual amino acid derivatives containing heterocycles and heteroatoms (S, O, N) via diethyl acetadimalonate as a glycine equivalent and carried out further chemical transformations to modify these unusual amino acid derivatives. Sulfur‐containing halo and alkene derivatives are prepared, and these substrates are further modified by Suzuki‐Miyaura cross‐coupling reaction, cross metathesis sequence and selective formation of sulfoxide and sulfone derivatives. We also report oxazole‐containing amino acid derivative by using TosMIC.
Highly Efficient and Ultralong Afterglow Emission with Anti‐Thermal Quenching from CsCdCl3 : Mn Perovskite Single Crystals
Authors: Tang Zhe , Liu Runze , Chen Junsheng , Zheng Daoyuan , Zhou Panwang , Liu Siping , Bai Tianxin , Zheng Kaibo , Han Keli , Yang Bin 
Published: October 2022

AbstractTriplet exciton‐based long‐lived phosphorescence is severely limited by the thermal quenching at high temperature. Herein, we propose a novel strategy based on the energy transfer from triplet self‐trapped excitons to Mn2+ dopants in solution‐processed perovskite CsCdCl3. It is found the Mn2+ doped hexagonal phase CsCdCl3 could simultaneously exhibit high emission efficiency (81.5 %) and long afterglow duration time (150 s). Besides, the afterglow emission exhibits anti‐thermal quenching from 300 to 400 K. In‐depth charge‐carrier dynamics studies and density functional theory (DFT) calculation provide unambiguous evidence that carrier detrapping from trap states (mainly induced by Cl vacancy) to localized emission centers ([MnCl6]4−) is responsible for the afterglow emission with anti‐thermal quenching. Enlightened by the present results, we demonstrate the application of the developed materials for optical storage and logic operation applications.
The Covalent Functionalization of Surface‐Supported Graphene: An Update
Authors: Wetzl Cecilia , Silvestri Alessandro , Garrido Marina , Hou Hui‐Lei , Criado Alejandro , Prato Maurizio 
Published: October 2022

AbstractIn the last decade, the use of graphene supported on solid surfaces has broadened its scope and applications, and graphene has acquire a promising role as a major component of high‐performance electronic devices. In this context, the chemical modification of graphene has become essential. In particular, covalent modification offers key benefits, including controllability, stability, and the facility to be integrated into manufacturing operations. In this Review, we critically comment on the latest advances in the covalent modification of supported graphene on substrates. We analyze the different chemical modifications with special attention to radical reactions. In this context, we review the latest achievements in reactivity control, tailoring electronic properties, and introducing active functionalities. Finally, we extended our analysis to other emerging 2D materials supported on surfaces, such as transition metal dichalcogenides, transition metal oxides, and elemental analogs of graphene.
Electrosynthetic C−O Bond Activation in Alcohols and Alcohol Derivatives
Authors: Villo Piret , Shatskiy Andrey , Kärkäs Markus D. , Lundberg Helena 
Published: October 2022

AbstractAlcohols and their derivatives are ubiquitous and versatile motifs in organic synthesis. Deoxygenative transformations of these compounds are often challenging due to the thermodynamic penalty associated with the cleavage of the C−O bond. However, electrochemically driven redox events have been shown to facilitate the C−O bond cleavage in alcohols and their derivatives either through direct electron transfer or through the use of electron transfer mediators and electroactive catalysts. Herein, a comprehensive overview of preparative electrochemically mediated protocols for C−O bond activation and functionalization is detailed, including direct and indirect electrosynthetic methods, as well as photoelectrochemical strategies.
Catalytic Ammonia Oxidation to Dinitrogen by a Nickel Complex
Authors: Stephens David N. , Szilagyi Robert K. , Roehling Paige N. , Arulsamy Navamoney , Mock Michael T. 
Published: October 2022

AbstractWe report a nickel complex for catalytic oxidation of ammonia to dinitrogen under ambient conditions. Using the aryloxyl radical 2,4,6‐tri‐tert‐butylphenoxyl (tBu3ArO⋅) as a H atom acceptor to cleave the N−H bond of a coordinated NH3 ligand up to 56 equiv of N2 per Ni center can be generated. Employing the N‐oxyl radical 2,2,6,6‐(tetramethylpiperidin‐1‐yl)oxyl (TEMPO⋅) as the H‐atom acceptor, up to 15 equiv of N2 per Ni center are formed. A bridging Ni‐hydrazine product identified by isotopic nitrogen (15N) studies and supported by computational models indicates the N−N bond forming step occurs by bimetallic homocoupling of two paramagnetic [Ni]−NH2 fragments. Ni‐mediated hydrazine disproportionation to N2 and NH3 completes the catalytic cycle.
Fluorescence and colorimetric‐based sensing of Hg2+ and Cu2+ using rhodamine derivative
Authors: Hu Jian‐Peng , Lin Qi , Yao Hong , Zhang You‐Ming , Wei Tai‐Bao 
Published: October 2022

Effective and simultaneous detection of multimetal ions has been achieved by a colorimetric and fluorometric sensor (RA), which was fabricated between rhodamine hydrazide and 9‐anthraldehyde through a simple Schiff base reaction. Sensor RA provided an effective platform for reliable detection of Hg2+ with a detection limit as low as 9.978 × 10−9 and displayed fabulous sensitivity for Cu2+ with a low limit of detection (LOD) of 4.81 × 10−8 M of detection limit toward Cu2+. Among them, the detection limit for heavy metal mercury ions has reached the ultrasensitive level. The sensing mechanism between RA and metal ions was further confirmed by 1H NMR and IR titration experiments and high‐resolution mass spectrometry and discussed from a theoretic level based on crystal structures and density functional theory (DFT) calculations. The switch in turn‐on signaling was attributed to the spiro‐lactam and ring‐opened form of RA and was accompanied by significant changes in ultraviolet spectroscopy and fluorescent spectrum.
Biosynthesis of astaxanthin by using industrial yeast
Authors: Zhou Dawei , Yang Xinyi , Wang Hanxiao , Jiang Yujia , Jiang Wankui , Zhang Wenming , Jiang Min , Xin Fengxue 
Published: October 2022

AbstractAstaxanthin has been widely applied in feed additives and the medical industry due to its antioxidant, coloring, and health‐promoting properties. Traditional processes for astaxanthin production mainly include natural extraction and chemical synthesis; however, they cannot meet the growing market demand and consumers’ requirements for natural products. Microbial synthesis of astaxanthin offers a promising alternative. Many natural sources of astaxanthin, such as Xanthophyllomyces dendrorhous, and genetically modified yeasts, such as Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus have been isolated and contructed. Various strategies such as mutagenesis, genetic modification, and fermentation regulation have been developed to increase the astaxanthin production from industrial yeast. Due to the broad substrate spectrum of industrial yeast, many low‐cost resources have also been explored for astaxanthin production. Accordingly, this review will mainly summarize the progress of research into the production of astaxanthin with yeasts, analyze factors affecting astaxanthin synthesis in metabolic pathways, and propose methods and strategies to achieve high yields of astaxanthin from yeasts. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.
Does central environmental protection inspection improve corporate environmental, social, and governance performance? Evidence from China
Authors: Wang Lin , Le Quanquan , Peng Manru , Zeng Huixiang , Kong Lingyao 
Published: October 2022

AbstractThe analysis of the impact of central environmental protection inspection (CEPI) on corporate environmental, social, and governance（ESG) performance is important for reforming the operation mechanism of CEPI to improve its effectiveness and fundamentally enhance corporate ESG to achieve sustainable development. This paper empirically examines the mechanism of the impact of CEPI on corporate ESG performance using a multi‐period difference‐in‐differences (DID) model with a research sample of 674 listed firms in China from 2013 to 2018. The results show that CEPI can significantly improve corporate ESG performance, and the strengthening of regulation by local governments is a potential channel. The important internal governance mechanisms of foreign and institutional investor shareholding, as well as the external governance mechanisms of analyst following and media coverage, can effectively strengthen the improvement effects. Further analysis also reveals that there are differences in the effectiveness of different inspection approaches, with “look‐back” inspection outperforming routine inspection. Heterogeneity at the firm and regional levels is also a critical factor in causing differences in the effectiveness of ESG performance improvements. This work not only enriches the research on the micro‐effects of CEPI on firms but also provides a reference for firms to improve their ESG performance and use ESG for investment and business decisions.
Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors
Authors: Xu Jiaqian , Peng Lijie , Guo Cuiping , Xu Fang , Lin Dong‐Shi , Tang Yi , Li Zhengqiu 
Published: October 2022

AbstractCompetitive proteome profiling is a powerful approach for the identification of targets of small molecules. This approach usually employs an inhibitor‐derived probe or a cysteine‐reactive probe such as an IA‐alkyne in a comparison between inhibitor‐treated and untreated samples, thus enabling distinction between genuine targets and nonspecific labeling. We have developed an active probe derived from an EGFR inhibitor, afatinib, and a cysteine reactive probe, an alkyne‐containing α,β‐unsaturated amide, to compare their characterization of cellular targets. In both approaches, myosin heavy chain 9 (MYH9) was identified as an off‐target. Subsequent functional validation experiments suggested that MYH9 might be involved in the function of afatinib.
Successful desensitization after hypersensitivity reaction to cisplatin in a patient with nasopharyngeal carcinoma
Authors: Kinouchi Arisa , Ishii Hiroki , Sakamoto Kaname , Sakurai Daiju 
Published: October 2022

AbstractHypersensitivity reaction to cisplatin can result in discontinuation of chemoradiotherapy in patients with head and neck squamous cell carcinoma. We describe a patient with nasopharyngeal carcinoma who developed cisplatin hypersensitivity and was successfully treated with cisplatin desensitization. Furthermore, it had little impact on the therapeutic performance of cisplatin‐combined chemoradiotherapy.