bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic metabolome profiling uncovers potential TOR signaling genes
Authors: Reichling Stella , Doubleday Peter F , Germade Tomas , Bergmann Ariane , Loewith Robbie , Sauer Uwe , Holbrook-Smith Duncan 
Published: July 2022

AbstractAlthough the genetic code of the yeastSaccharomyces cerevisiaewas sequenced 25 years ago, the characterization of the roles of genes within it is far from complete. The lack of a complete mapping of functions to genes hampers systematic understanding of the biology of the cell. The advent of high-throughput metabolomics offers a unique approach to uncovering gene function with an attractive combination of cost, robustness, and breadth of applicability. Here we used flow-injection time-of-flight mass spectrometry (FIA-MS) to dynamically profile the metabolome of 164 loss-of-function mutants in TOR and receptor or receptor-like genes under a time-course of rapamycin treatment, generating a dataset with over 7,000 metabolomics measurements. In order to provide a resource to the broader community, those data are made available for browsing through an interactive data visualization app hosted athttps://rapamycin-yeast-metabolome.herokuapp.com/. We demonstrate that dynamic metabolite responses to rapamycin are more informative than steady state responses when recovering known regulators of TOR signaling, as well as identifying new ones. Deletion of a subset of the novel genes causes phenotypes and proteome responses to rapamycin that further implicate them in TOR signaling. We found that one of these genes,CFF1, was connected to the regulation of pyrimidine biosynthesis through URA10. These results demonstrate the efficacy of the approach for flagging novel potential TOR signaling-related genes and highlights the utility of dynamic perturbations when using functional metabolomics to deliver biological insight.
Three species ofMelaleucafrom Western Australia are highly susceptible toAustropuccinia psidiiin controlled inoculations
Authors: Martino Alyssa M , Park Robert F , Tobias Peri A 
Published: July 2022

AbstractAustropuccinia psidii, the fungus causing myrtle rust, was detected in Western Australia for the first time in June 2022. Few Western Australian plant species have been screened for response to the pathogen.Melaleuca thyoides, Melaleuca marginataandMelaleuca leucadendragrown from seeds sourced from Western Australian populations were all highly susceptible to an isolate of the pathogen from eastern Australia.
Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature
Authors: Moreno-Chicano Tadeo , Carey Leiah M. , Axford Danny , Beale John H. , Doak R. Bruce , Duyvesteyn Helen M. E. , Ebrahim Ali , Henning Robert W. , Monteiro Diana C. F. , Myles Dean A. , Owada Shigeki , Sherrell Darren A. , Straw Megan L. , Šrajer Vukica , Sugimoto Hiroshi , Tono Kensuke , Tosha Takehiko , Tews Ivo , Trebbin Martin , Strange Richard W. , Weiss Kevin L. , Worrall Jonathan A. R. , Meilleur Flora , Owen Robin L. , Ghiladi Reza A. , Hough Michael A. 
Published: July 2022

Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies. In the case of metalloenzymes that are highly sensitive to radiation damage, such room-temperature experiments can present challenges, including increased rates of X-ray reduction of metal centres and site-specific radiation-damage artefacts, as well as in devising appropriate sample-delivery and data-collection methods. It can also be problematic to compare structures measured using different crystal sizes and light sources. In this study, structures of a multifunctional globin, dehaloperoxidase B (DHP-B), obtained using several methods of room-temperature crystallographic structure determination are described and compared. Here, data were measured from large single crystals and multiple microcrystals using neutrons, X-ray free-electron laser pulses, monochromatic synchrotron radiation and polychromatic (Laue) radiation light sources. These approaches span a range of 18 orders of magnitude in measurement time per diffraction pattern and four orders of magnitude in crystal volume. The first room-temperature neutron structures of DHP-B are also presented, allowing the explicit identification of the hydrogen positions. The neutron data proved to be complementary to the serial femtosecond crystallography data, with both methods providing structures free of the effects of X-ray radiation damage when compared with standard cryo-crystallography. Comparison of these room-temperature methods demonstrated the large differences in sample requirements, data-collection time and the potential for radiation damage between them. With regard to the structure and function of DHP-B, despite the results being partly limited by differences in the underlying structures, new information was gained on the protonation states of active-site residues which may guide future studies of DHP-B.
Study of strain induced martensite and its reversal on sensitization behaviour of metastable austenitic stainless steel
Authors: Wanjari Devashish Vikas , Patil Awanikumar P. , Singh Sushil Kumar , Ullah Inayat , Shukla Sourabh 
Published: July 2022

PurposeThe purpose of the present study is to investigate the effects of strain-induced martensite (SIM) and its reversal on metastable austenitic stainless steel (MASSs) through the analysis of metallurgical and sensitisation behaviour.Design/methodology/approachIn the present investigation, the samples of Cr-Mn ASS (also known as MASSs) including 15%, 30% and 50% cold worked, solution annealed samples with and without thermal ageing (at 700°C for 3 h) were analysed with the help of X-ray diffraction analysis, microstructure examination and electrochemical behaviour. The scanning electron microscope (SEMJOEL 6380 A) was used to examine the microstructure of the sample, and the double-loop electrochemical potentiokinetic reactivation test was used to determine the degree of sensitisation (DOS) in the samples. The cold worked solution annealed samples without thermal ageing are named as CR15, CR30 and CR50, respectively, and the samples with thermal ageing are named as CR15_TA, CR30_TA and CR50_TA, respectively.FindingsIn CR15, CR30 and CR50 samples, the DOS increased with increase in the extent of cold working, which was attributable to passivation deterioration. Because of the high degree of passivation at the grain boundaries, the DOS of CR15_TA and CR30_TA were practically identical. The DOS in the CR50_TA sample, on the other hand, was lowered due to SIM recovery in the austenite.Originality/valueThe present study sheds light on how to choose the right cold working percentage to avoid sensitisation in MASSs during the fabrication of metal forming components.
Image processing methods decision mechanism for surveillance applications with UAVs
Authors: Ayar Murat , Dalkiran Alper , Kale Utku , Nagy András , Karakoc Tahir Hikmet 
Published: July 2022

Purpose
The use of unmanned aerial vehicles (UAVs) has significantly increased in the past decade and nowadays is being used for various purposes such as image processing, cargo transport, archaeology, agriculture, manufacturing, health care, surveillance and inspections. For this reason, using the appropriate image processing method for the intended use of UAVs increases the study’s success. This study aims to determine the most suitable one among the innovative methods that constitute the image processing system for a UAV to be used for surveillance purposes.

Design/methodology/approach
Analytical hierarchy process has been used in the solution of the decision problem to be handled in three stages, namely, platform, architecture and method. The most suitable alternative and the effect weights of these criteria results were determined at each stage.

Findings
As a result of this study, Jetson TX2 was determined as the most suitable embedded platform, ResNet is the optimum architecture and Faster R-convolutional neural networks was the best method in the image processing layer for a system that will provide surveillance with image processing method using UAV.

Practical implications
In UAV designs, where multiple hardware and software choices and system combinations exist, multi-criteria decision-making (MCDM) approaches can be used as a system decision mechanism.

Originality/value
The novelty of this work comes from the application of MCDM methods that are used as a multi-layered decision mechanism in UAV design.
Close-proximity, conservative extrapolation of load spectra
Authors: Rodzewicz Miroslaw 
Published: July 2022

Purpose
The purpose of this paper is to present the author’s method of conservative load spectrum (LS) derivation and close-proximity LS extrapolation applying a correction for measurement uncertainty caused by too low sampling frequency or signal noise, which may affect the load histories collected during the flying session and cause some recorded load increments to be lower than the actual values.

Design/methodology/approach
Having in mind that the recorded load signal is burdened with some measurement error, a conservative approach was applied during qualification of the recorded values into 32 discrete load-level intervals and derivation of 32 × 32 half-cycle arrays. A part of each cell value of the half-cycle array was dispersed into the neighboring cells placed above by using a random number generator. It resulted in an increase in the number of load increments, which were one or two intervals higher than those resulting from direct data processing. Such an array was termed a conservative clone of the actual LS. The close-proximity approximation consisted of multiplication of the LSs clones and their aggregation. This way, the LS for extended time of operation was obtained. The whole process was conducted in the MS Excel environment.

Findings
Fatigue life calculated for a chosen element of aircraft structure using conservative LS is about 20%–60% lower than for the actual LS (depending on the applied value of dispersion coefficients used in the procedure of LSs clones generation). It means that such a result gives a bigger safety margin when operational life of the aircraft is estimated or when the fatigue test for an extended operational period is programed based on a limited quantity of data from a flying session.

Originality/value
This paper presents a proposal for a novel, conservative approach to fatigue life estimation based on the short-term LS derived from the load signal recorded during the flying session.
When does a supplier tolerate resellers’ opportunistic behaviors? Transaction benefit and cost perspectives
Authors: Lee Youngsu 
Published: July 2022

Purpose
The purpose of this study is to examine tolerance of channel partners’ opportunistic behaviors as a viable governance mechanism and to test contingent transaction benefit and cost factors to determine tolerance of opportunistic behaviors.

Design/methodology/approach
Through the theoretical lenses of governance value analysis and transaction cost economics, this study theorizes that a supplier’s tolerance of its reseller’s opportunistic behaviors should depend on transaction benefit factors (e.g. new product creativity and marketing program creativity) and transaction cost factors (e.g. performance ambiguity and opportunity cost). The author empirically tests the moderation model using data from a large-scale survey of 141 mobile phone suppliers in South Korea.

Findings
The empirical results largely support the predictions on the moderating effects. For transaction benefit factors, marketing program creativity increases the supplier’s tolerance, while new product creativity does not increase the supplier’s tolerance. For transaction cost factors, the supplier’s concerns about opportunity cost increase the level of tolerance, while performance ambiguity of a business partner decreases the tolerance level.

Research limitations/implications
Theorizing opportunistic behaviors as a policy variable subject to benefit-cost assessment rather than an assumption provides new insights to interfirm governance research.

Originality/value
To the best of the author’s knowledge, this study is the first kind to consider transaction benefit and cost factors together in a single contingency framework in tolerance research. Also, this research provides a new perspective on a microlevel marketing factor (i.e. creativity) as an influential factor in governance mechanisms.
“The moment he realized he needed our joint assistance made the difference”: a multiple case study into working elements in the supervision of probation clients with debt
Authors: Beek G. van , de Vogel Vivienne , van de Mheen Dike 
Published: July 2022

Purpose
Little is known about effective supervision of offenders with debt. This multiple case study aims to gain insight into working elements in offender supervision on debt. This is important for probation officers to choose the most effective interventions in daily offender supervision.

Design/methodology/approach
This study included five best practice cases based on both interviews with involved professionals and clients and client file information. One case was described in detail to illustrate what probation officers and clients encounter when working on debt. All five cases were analyzed thematically using pattern matching techniques and crosscase syntheses on debt background, current supervision, barriers and working elements.

Findings
Organization processes and lack of aftercare hinder effective supervision. Close collaboration with other professionals (e.g. debt counselors) is important in supervising clients with debt. The client’s own behavior and motivation for supervision are crucial in the success of debt supervision and can be both hindering and effective. Working elements in supervision depend on personal characteristics of professionals involved and on the extent to which elements of a working alliance, particularly trust and bonding, are built.

Practical implications
Support and facilitation from probation organizations regarding primary conditions and collaboration, training professionals in methods of stimulating clients motivation and an effective working alliance are essential to supervise clients with debt adequately.

Originality/value
To the best of the authors’ knowledge, no other in-depth study has yet been conducted on working elements in supervision of probationers with debt.
Time headway distribution analysis of naturalistic road users based on aerial datasets
Authors: Yu Ruilin , Zhang Yuxin , Wang Luyao , Du Xinyi 
Published: July 2022

Purpose
Time headway (THW) is an essential parameter in traffic safety and is used as a typical control variable by many vehicle control algorithms, especially in safety-critical ADAS and automated driving systems. However, due to the randomness of human drivers, THW cannot be accurately represented, affecting scholars’ more profound research.

Design/methodology/approach
In this work, two data sets are used as the experimental data to calculate the goodness-of-fit of 18 commonly used distribution models of THW to select the best distribution model. Subsequently, the characteristic parameters of traffic flow are extracted from the data set, and three variables with higher importance are extracted using the random forest model. Combining the best distribution model parameters of the data set, this study obtained a distribution model with adaptive parameters, and its performance and applicability are verified.

Findings
In this work, two data sets are used as the experimental data to calculate the goodness-of-fit of 18 commonly used distribution models of THW to select the best distribution model. Subsequently, the characteristic parameters of traffic flow are extracted from the data set, and three variables with higher importance are extracted using the random forest model. Combining the best distribution model parameters of the data set, this study obtained a distribution model with adaptive parameters, and its performance and applicability are verified.

Originality/value
The results show that the proposed model has a 62.7% performance improvement over the distribution model with fixed parameters. Moreover, the parameter function of the distribution model can be regarded as a quantitative analysis of the degree of influence of the traffic flow state on THW.
Firm objects: new realist insights into the sociohistorical ontology of the business enterprise
Authors: Reveley James 
Published: July 2022

PurposeBy engaging with recent debates between management historians over social constructionism, this paper aims to show the merits of adopting a new realist ontology of the business enterprise. In contrast with ANTi-History, the purpose is to provide a philosophically rigorous conception of social objects and to argue that enterprises are a member of this category.Design/methodology/approachInsights from Maurizio Ferraris’s documentality theory and Graham Harman’s philosophy of social objects are used to identify the ontological forming ground and developmental pathway of an Antipodean stevedoring company that operated prior to the deregulation of New Zealand’s ports in 1989.FindingsWith regard to social entities in general and firms in particular, continental philosophy’s resurgent realist movement provides a history-aware social ontology that incorporates the grain of truth lying within social constructionism. As exemplified by the writings of Ferraris and Harman, realism provides a viable conception of social objects and, in so doing, a more coherent ontological foundation for the business enterprise than the relational ontology embraced by management history ANTians.Originality/valueBy drawing on two realist perspectives hitherto neglected by management historians, this paper resolves disagreements about the ontology of the business enterprise.
Do mobile device and mobile app innovations trigger lifestylisations? Insights from consumers in developing countries
Authors: Odoom Raphael 
Published: July 2022

Purpose
The exponential growth of smartphones is complemented by an astronomical development of mobile apps that have been changing ways in which humans interact with each other, as well as how brands and customers interact. This study aims to examine the effect of mobile device and mobile app innovations on user lifestylisation among consumers from developing countries.

Design/methodology/approach
Through a qualitative approach, data was collected via focus group interviews from 32 participants from across 15 developing countries who were largely emerging cosmopolitans.

Findings
Using the thematic analysis technique, the study finds that consumer lifestylisations, based on consumption and utilisation of mobile devices and mobile apps, hinge on either pre-purchase considerations or post-purchase discoveries that stem from a bouquet of hedonic and/or utilitarian motivations. Two consumer categories are identified, with each category exhibiting unique patterns.

Originality/value
The empirical findings provide valuable theoretical contributions to new knowledge as well as practical implications for mobile gadget manufacturers and mobile app developers domiciled, or those aiming to establish their presence, in developing economies.
Today’s workforce is evolving before your eyes – it’s a moment businesses can’t afford to miss
Authors: Alvino Nicole 
Published: July 2022

Purpose
Employee attitudes are changing: it is time to embrace Gigification. Sure enough, employees feel empowered and re-energized to set their own path in life, professionally and personally, but the idea that just offering “flexibility” on where employees work will fix things, is a knee jerk reaction, and does not reflect the nuance of what is really happening. The idea that a job is no longer for life, was seen as a negative turn of phrase, to today’s it is a positive and a way of life to embrace, that means they can take the “gigs” they want, that meet their needs, and move on when it suits them.

Design/methodology/approach
For employers that want to attract the best talent, a rethink is needed addressing the needs of employees, communication, management style, benefits and the digital employee experience, from onboarding to exit.

Findings
This byline will pull in data from Firstup’s latest employee survey of 23,105 workers from across the UK, USA and Europe, and suggest a number of areas that companies can start to look at to embrace gamification, including changes to company culture, using technology to create personal experiences, upskilling the soft skills and new forms of internal communication.

Originality/value
The author offers unique insight based on her extensive experience in this space.
Tourism value chain: synthesizing value webs to support tourism development and planning
Authors: Sutomo Yoan Adi Wibowo , Sianipar Corinthias P.M. , Basu Mrittika , Onitsuka Kenichiro , Hoshino Satoshi 
Published: July 2022

Purpose
Tourism encompasses multiple activities along with actors, locations and roles involved. Thus, tourism development and planning should consider value-added activities that form a tourism value chain (TVC). In the literature, the conceptual and practical understanding of TVC is significantly diverse, making it challenging to comprehend the entire framework. This study aims to synthesize a general TVC framework.

Design/methodology/approach
This review uses the Preferred Reporting Items for Systematic Reviews and Meta-Analyses protocol to search for and select relevant literature in two databases (Scopus® and Web of Science™). Covering six aspects (logic, scope, value, perspective, type and scale), this study systematically analyzes the selected empirical studies to synthesize a functional framework of TVC as the basis to explore insights for effective TVC practices.

Findings
The results show that existing empirical studies have adopted four types of logic (supply logic, destination logic, global value chain and tourism global value chain), which complement each other in explaining the entire concept of TVC. Besides, the TVC concept is applicable on various scales and in different types of tourism with slight practical differences. Furthermore, values appear in various forms and offer benefits for suppliers and consumers as primary actors in TVC. Findings for the six aspects form tourism value webs as the functional framework of TVC.

Originality/value
This study offers a wide-ranging review of various empirically implemented TVC concepts for comprehensive TVC-based tourism development and planning. This review sets a theoretical foundation for future studies to improve the body of knowledge of TVC and tourism development and planning in general.