bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vegetation of natural and stabilized riverbanks and early effects of removal of bank fixation
Authors: Seele‐Dilbat Carolin , Kretz Lena , Wirth Christian 
Published: February 2022

AbstractThe majority of rivers in Europe has been dramatically altered in terms of their morphology and hydrology with severe consequences for the diversity and ecological functioning of the rivers and their floodplains. Consequently, an increasing number of river reaches has been restored over the past decades, often including the removal of bank fixation to reinitiate bank erosion and allowing for a rewidening of the rivers' cross‐section. However, monitoring in detail the effect of such a measure on riverine vegetation is scarce. Using a Before‐After‐Control‐Impact (BACI) design, we analysed the early changes of the vegetation 2 years after the removal of bank fixation at the Mulde River (central Germany). The results were compared with two types of control sites, representing a nonrestored as well as a natural riverbank that has never been fixed. We analysed differences in taxonomic and functional composition of the vegetation between bank types as well as community turnover after restoration. Two years after restoration, the vegetation differed clearly from the nonrestored sites. A substantial proportion of the community, especially the newly established species, became more similar to the natural bank community. Most importantly, spatiotemporal heterogeneity of the vegetation increased after removal of bank fixation, being an indicator for re‐established dynamic processes similar to the natural site. The presented data serve as a baseline for a long‐term monitoring and quantitative meta‐analysis of restoration effects.
Serum/glucocorticoid‐regulated kinase 1‐targeted transient receptor potential oxalate subtype 1 regulates bladder smooth muscle cell proliferation due to bladder outlet obstruction in mice via activated T cell nuclear factor transcription factor 2
Authors: He Jiangshu , Yang Jin , Chen Lin , He Pinglin , Liu Xun , Wang Kai , Dong Taotao , Li Jia , Ma Xudong , Bastian Amend , Arnulf Stenzl 
Published: February 2022

AbstractBladder outlet obstruction (BOO) is a type of chronic disease that is mainly caused by benign prostatic hyperplasia. Previous studies discovered the involvements of both serum/glucocorticoid‐regulated kinase 1 (SGK1) and activated T cell nuclear factor transcription factor 2 (NFAT2) in the proliferation of smooth muscle cells after BOO. However, the relationship between these two molecules is yet to be explored. Thus, this study explored the specific mechanism of the SGK1‐NFAT2 signaling pathway in mouse BOO‐mediated bladder smooth muscle cell proliferation in vivo and in vitro. In vivo experiments were performed by suturing 1/2 of the external urethra of female BALB/C mice to cause BOO for 2 weeks. In vitro, mouse bladder smooth muscle cells (MBSMCs) were treated with dexamethasone (Dex) or dexamethasone + SB705498 for 12 h and were transfected with SGK1 siRNA for 48 h. The expression and distribution of SGK1, transient receptor potential oxalate subtype 1 (TRPV1), NFAT2, and proliferating cell nuclear antigen (PCNA) were measured by Western blotting, polymerase chain reaction, and immunohistochemistry. The relationship between SGK1 and TRPV1 was analyzed by coimmunoprecipitation. The proliferation of MBSMCs was examined by 5‐ethynyl‐2′‐deoxyuridine and cell counting kit 8 assays. Bladder weight, smooth muscle thickness, and collagen deposition in mice after 2 weeks of BOO were examined. Bladder weight, smooth muscle thickness, the collagen deposition ratio, and the expression of SGK1, TRPV1, NFAT2, and PCNA were significantly increased in mice after 2 weeks of BOO. Compared with the control, 10 μM Dex promoted the expression of these four molecules and the proliferation of MBSMCs. After inhibiting TRPV1, only the expression of SGK1 was not affected, and the proliferation of MBSMCs was inhibited. After silencing SGK1, the expression of these four molecules and the proliferation of MBSMCs decreased. Coimmunoprecipitation suggested that SGK1 acted directly on TRPV1. In this study, SGK1 targeted TRPV1 to regulate the proliferation of MBSMCs mediated by BOO in mice through NFAT2 and then affected the process of bladder remodeling after BOO. This finding may provide a strategy for BOO drug target screening.
Melatonin Prevents Cartilage Degradation in Early-Stage Osteoarthritis Through Activation of miR-146a/NRF2/HO-1 Axis
Authors: Zhou Xinfeng , Zhang Yijian , Hou Mingzhuang , Liu Hao , Yang Huilin , Chen Xi , Liu Tao , He Fan , Zhu Xuesong 
Published: February 2022

ABSTRACT
Reactive oxygen species (ROS) are implicated in induction of inflammatory response and cartilage degradation in osteoarthritis (OA). Melatonin has been shown to improve the chondrogenic differentiation and promote cartilage matrix synthesis in mesenchymal stem cells. However, the underlying mechanisms of melatonin-regulated antioxidant activity in OA cartilage are not known. The aim of this study was to explore the effect of melatonin on nuclear factor-erythroid 2-related factor 2 (NRF2), a key antioxidant transcription factor, and its target antioxidant genes in early-stage OA cartilage. Primary chondrocytes were isolated from rats with surgically induced OA. In vitro treatment of melatonin significantly increased cartilage matrix synthesis and upregulated antioxidant enzymes, mainly heme oxygenase 1 (HO-1), while decreasing matrix degradation enzymes and intracellular ROS. In vivo intraarticular injection of melatonin effectively ameliorated cartilage degeneration in an experimental rat OA model. Inhibition of melatonin membrane receptors by Luzindole or 4-P-PDOT reversed the beneficial effects of melatonin on cartilage matrix synthesis, implying that melatonin receptor-mediated pathway is involved in its anti-arthritic effects. Interestingly, melatonin showed no significant effect on the mRNA level of Nrf2 but significantly increased its protein level. Silencing of Nrf2 or HO-1 expression abolished the protective effects of melatonin, as shown by increased ROS levels and matrix degradation enzyme expression. Microarray assays revealed that miR-146a, a predicted target for Nrf2, was significantly upregulated in OA chondrocytes but was markedly reduced by melatonin treatment. Overexpression of miR-146a diminished the protective effects of melatonin by inhibiting NRF2 expression and aggravating OA-induced cartilage degradation. These findings demonstrate that melatonin supports the anabolic metabolism of cartilage matrix in OA chondrocytes by enhancing the protein levels of NRF2 via suppressing miR-146a. Melatonin-mediated activation of the NRF2/HO-1 axis prevents cartilage degeneration and represents a promising therapeutic target for treatment of early-stage OA. © 2022 American Society for Bone and Mineral Research (ASBMR).
Abstract
Illustration of the protective effect of melatonin on treating OA. Intraarticular injection of melatonin prevents the progress of rats OA by rescuing the cartilage ECM loss. Mechanically, melatonin promotes the translocation of NRF2 via inhibiting the expression of miR-146a. Activated NRF2 signaling pathway enhances the antioxidant enzyme HO-1, which inhibits excess intracellular ROS to regulate cartilage matrix metabolism.
The strength and selectivity of perfluorinated nano‐hoops and buckybowls for anion binding and the nature of anion‐π interactions
Authors: Fan Dan , Du Juan , Dang Jingshuang , Wang Changwei , Mo Yirong 
Published: February 2022

AbstractPerfluorinated cycloparaphenylenes (F‐[n]CPP, n = 5–8), boron nitride nanohoop (F‐[5]BNNH), and buckybowls (F‐BBs) were proposed as anion receptors via anion‐π interactions with halide anions (Cl−, Br− and I−), and remarkable binding strengths up to −294.8 kJ/mol were computationally verified. The energy decomposition approach based on the block‐localized wavefunction method, which combines the computational efficiency of molecular orbital theory and the chemical intuition of ab initio valence bond theory, was applied to the above anion‐π complexes, in order to elucidate the nature and selectivity of these interactions. The overall attraction is mainly governed by the frozen energy component, in which the electrostatic interaction is included. Remarkable binding strengths with F‐[n]CPPs can be attributed to the accumulated anion‐π interactions between the anion and each conjugated ring on the hoop, while for F‐BBs, additional stability results from the curved frameworks, which distribute electron densities unequally on π‐faces. Interestingly, the strongest host was proved to be the F‐[5]BNNH, which exhibits the most significant anisotropy of the electrostatic potential surface due to the difference in the electronegativities of nitrogen and boron. The selectivity of each host for anions was explored and the importance of the often‐overlooked Pauli exchange repulsion was illustrated. Chloride anion turns out to be the most favorable anion for all receptors, due to the smallest ionic radius and the weakest destabilizing Pauli exchange repulsion.
Dominant bacteria and influencing factors of early intestinal colonization in very low birth weight infants: A prospective cohort study
Authors: Lu Yanbo , Cai Xiaohong , Zheng Yao , Lyv Qin , Wu Junhua 
Published: February 2022

AbstractBackgroundThe intestine of newborns is colonized by bacteria immediately after birth. This study explored dominant bacteria and influencing factors of early intestinal colonization in the early life of very low birth weight infants (VLBWI).MethodsWe enrolled 81 VLBWI and collected anal swabs at 24 h, 7th, 14th and 21st day after birth. We conducted bacterial culture for anal swabs, then selected the colony with obvious growth advantages in the plate for further culture and identification. Afterward, we analyzed the distribution and influencing factors of intestinal dominant microbiota combined with clinical data.ResultsA total of 300 specimens were collected, of which 62.67% (188/300) had obvious dominant bacteria, including 29.26% (55/188) Gram‐positive bacteria and 70.74% (133/188) Gram‐negative bacteria. The top five bacteria with the highest detection rates were Klebsiella pneumoniae, Escherichia coli, Enterococcus faecium, Enterococcus faecalis and Serratia marcescens. Meconium‐stained amniotic fluid and chorioamnionitis were correlated with intestinal bacterial colonization within 24 h of birth. Mechanical ventilation and antibiotics were independent risk factors affecting colonization. Nosocomial infection of K. pneumoniae and S. marcescens were associated with intestinal colonization. The colonization rates of K. pneumoniae, E. coli, E. faecium, and E. faecalis increased with the birth time.ConclusionsThe colonization rate in the early life of VLBWI increased over time and the predominant bacteria were Gram‐negative bacteria. Meconium‐stained amniotic fluid and chorioamnionitis affect intestinal colonization in early life. Mechanical ventilation and antibiotics were independent risk factors for intestinal bacterial colonization. The nosocomial infection of some bacteria was significantly related to intestinal colonization.
Wellbeing, coping with homeschooling, and leisure behavior at different COVID‐19‐related lockdowns: A longitudinal study in 9‐ to 16‐year‐old German children
Authors: Poulain Tanja , Meigen Christof , Kiess Wieland , Vogel Mandy 
Published: February 2022

AbstractBackgroundSchool closures are an effective measure against the spread of Covid‐19. However, they pose a major challenge to children, especially to those from socially disadvantaged families. The present study compared the wellbeing, coping with homeschooling, and leisure behavior of children and adolescents at two different periods of school closures in Germany. Wellbeing was also compared with wellbeing before the pandemic.MethodsWithin the framework of the cohort study LIFE Child, 152 9‐ to 16‐year‐old children completed online surveys on wellbeing (KIDSCREEN‐27 scales on physical wellbeing, psychological wellbeing, and peer and social support), coping with homeschooling (concentration, motivation, fun, mastering of schoolwork, fear of bad marks), and leisure behavior (TV time, computer gaming time, indoor physical activity) during two COVID‐19‐related lockdowns in March 2020 (t1) and in January 2021 (t2). Data from both time points were compared using mixed‐effect models. Wellbeing was additionally compared with the wellbeing in 2019, before COVID‐19 (t0). We also assessed the effects of the socio‐economic status (SES) on all outcomes and changes between time points.ResultsAll considered wellbeing scores declined significantly between t0 and t1. Physical wellbeing decreased further between t1 and t2, while social support increased. Coping with homeschooling degraded significantly between t1 and t2, while leisure behavior did not change significantly. Lower SES was associated with lower physical wellbeing, poorer coping with homeschooling, longer computer gaming times, and a stronger decrease of concentration on schoolwork from t1 to t2.ConclusionRepeated school closures have a negative effect on already compromised physical wellbeing and coping with homeschooling, especially in children from lower social strata.
Dissipation and effect of glyphosate during composting of organic wastes
Authors: Grenier Vanessa , Moingt Matthieu , Lucotte Marc , Pitre Frederic E. 
Published: February 2022

AbstractThe addition of organic matter (OM) containing glyphosate during compost production, through the introduction of contaminated plant residues or sewage sludge, presents a risk of hindering the proper OM breakdown carried out by microorganisms and causing the accumulation of glyphosate or aminomethylphosphonic acid (AMPA). To measure the effect of glyphosate and glyphosate‐based herbicide (GBH) on OM decomposition as well as the dissipation of glyphosate to AMPA during composting, a controlled‐environment experiment was conducted using mesocosm‐scale vessels. Analytical‐grade (AG) glyphosate (150 mg kg–1) and GBH (VisionMAX) equivalent to the amounts applied in agricultural areas (300 mg kg–1) were added to a mixture of green residues, which were then composted for 112 d. Sampling after 2, 7, 28, and 112 d showed a negligible effect of glyphosate and GBH on physicochemical properties of the mixture (temperature, OM%, pH, total carbon [C], total nitrogen [N], and C/N ratio), ammonification, nitrification, and phosphate content. No differences between AG glyphosate and GBH treatments were measured. Glyphosate levels decreased significantly after 2 d to reach 53.1 and 71.1% of the initial content for the AG glyphosate and GBH treatments, respectively, and glyphosate dissipation was almost complete after 112 d of composting. Aminomethylphosphonic acid could not be detected at any time during the experiment regardless of the treatment. Our results show that conditions for OM decomposition were maintained despite the addition of glyphosate and suggest that only trace amounts of glyphosate or AMPA are likely to be present in mature compost.
Clinical characteristics and viral analysis of severe influenza A [H1N1]pdm09 in Guangzhou, 2019
Authors: Dou Min , Song Wenjun , Lin Yongping , Chen Qigao , Lu Chang , Liu Zhongmin 
Published: February 2022

AbstractTo understand the clinical characteristics of and analyze viral genes in patients with severe pneumonia due to [H1N1]pdm09 influenza virus in Guangzhou, 2019. The clinical data of 120 inpatients with laboratory‐confirmed influenza A [H1N1]pdm09 virus from January to March 2019 were collected and analyzed. The subjects were diagnosed according to the criteria of the “Diagnosis and Treatment Program of Influenza A H1N1 (third Edition 2009)” issued by the Ministry of Health and were divided into severe and nonsevere groups. Serum samples during fever were collected for cytokine analysis, and the viral genes were analyzed after the virus cultured in MDCK cells. The data were analyzed by SPSS 16 software, and the results of gene sequencing were analyzed by MEGA 6 software. Among the 120 inpatients, 36 (30%) were severe and 84 (70%) were nonsevere patients. The average age of severe patients was 53.11 ± 19.94 years, the average age of nonsevere patients, at 44.03 ± 24.47 years. There was no significant difference between the two groups (p < 0.05). There were significant differences in the rates of moist rales and dyspnea in critically ill patients (p < 0.05). There were significant differences in the white blood cell count (WBC), lactate dehydrogenase (LDH), creatine kinase (CK), serum creatinine (sCr), procalcitonin (PCT) and C‐reactive protein (CRP) in severe patients with type A H1N1. Chest radiologic findings in severe patients showed ground glass shadows or pulmonary solid changes, and the difference was statistically significant for pulmonary fibrosis. Chronic lung disease (52.8%) and cardiovascular disease (27.8%) were independent risk factors for severe disease (p < 0.05). There were significant differences in secondary infections by Staphylococcus aureus (11.1%), pulmonary Aspergillus (22%) and Acinetobacter baumannii (16.7%) in critically ill patients (p < 0.05). Serum IL‐8 in critically ill patients was significantly higher than those in nonsevere patients and healthy controls. The origin of virus strains in severe and nonsevere patients was the same, and there was no obvious mutation in the amino acid region of the antigenic site of the HA protein, but compared with the results of gene sequencing in previous years, the mutation sites showed a trend of annual accumulation. In conclusion, there was a high risk of severe pneumonia caused by H1N1 influenza A virus in Guangzhou in spring 2019. Long‐term continuous surveillance, prevention and control of the virus should be carried out to predict its epidemiology and distribution.
Effects of sand burial caused by ant nests on soil microbial biomass, basal respiration, and enzyme activity in/under biocrusts in vegetated areas of the Tennger Desert
Authors: Liu Yanmei , Jia Rongliang , Yang Hangyu , Xing Zisheng , Shi Guoxi , Cui Zheming 
Published: February 2022

AbstractSand burial of crusts, caused by ant burrowing, is a common phenomenon in desert landscapes, yet the potential impacts on soil processes are unknown. Three sand burial depths, 3.0–5.0 mm (deep burial), 1.0–1.5 mm (shallow burial), and 0 mm (control) affecting two dominant biocrusts (viz., cyanobacteria‐lichen crusts and moss crusts) sampled in the moist and dry seasons were selected to explore this issue in two vegetation areas of the Tennger Desert. We collected 180 samples from biocrust layers and topsoil to assess soil physicochemical properties, microbial biomass, basal respiration, and enzymes activities. The results showed that sand burial of crusts reduced soil microbial biomass, basal respiration, and enzyme activities, while deep burial caused a substantial decline in these parameters. Sand burial interfered with the biocrusts structure (e.g., rupture and flaking) and properties, subsequently causing soil‐nutrient deprivation and soil microclimate deterioration. These are major mechanisms for decreasing soil microbial biomass, basal respiration, and enzyme activities. Moss crusts had significantly higher levels of the microbial parameters than cyanobacteria‐lichen crusts following sand burial, indicating that biocrusts' tolerance to sand burial was synchronized with their progressive succession. In addition, soil microbial parameters were higher in natural vegetation areas than artificial vegetation areas following sand burial. Such observations suggest that high soil fertility could mitigate the negative effects of sand burial on soil processes. In conclusion, sand burial induces biocrust degradation and further disturbs surface soil processes in arid desert ecosystems.
Interaction of anti‐wear additiveTCPwith advanced bearing steels
Authors: Trivedi Hitesh K. , Rosado Lewis 
Published: February 2022

AbstractThe quest for high‐performance energy efficient aircraft turbine engines has led to the development of a number of high‐performance rolling element bearing materials and engine lubricants with the aim of providing superior mechanical component durability. The heat treatments/surface treatments used to achieve the desired physical and mechanical properties for these newer alloys can result in altered surface chemistry from currently used materials. Surface chemistry plays an important role during lubricant‐bearing material interactions and the formation of beneficial tribological films during component operation. The objective of this study was to analyse the tribo‐films formed on bearing surfaces and investigate the interaction of lubricant additives, specifically the phosphorus‐based anti‐wear additive tricresyl phosphate (TCP), with different bearing materials under relevant bearing operating conditions. Bearing tests were conducted on 208‐size (40 mm bore) angular contact bearings at 127°C and 154°C using gas turbine engine lubricants conforming to MIL‐PRF‐23699G at maximum Hertzian contact stresses of 3.1 GPa and 3.55 GPa. Bearing materials evaluated included AISI M50, M50NiL, nitrided M50NiL (N) and three variants of Pyrowear 675 with silicon nitride rolling elements. Tribo‐films were analysed using Energy dispersive X‐ray spectroscopy and Auger Electron spectroscopy. Results indicate that phosphorus‐rich anti‐wear tribo‐films form on all of the bearing materials studied. The applied thrust load and heat treatment had a significant effect on tribo‐film thickness. The study also suggests that current gas turbine engine lubricants formulated with TCP should form beneficial tribo‐films that enhance bearing fatigue life and performance.
An m‐Health telerehabilitation and health education program on physical performance in patients with hip fracture and their family caregivers: Study protocol for the ActiveHip+ randomized controlled trial
Authors: Mora‐Traverso Marta , Molina‐Garcia Pablo , Prieto‐Moreno Rafael , Borges‐Cosic Milkana , Cruz Guisado Victor , del Pino Algarrada Rogelio , Moreno‐Ramírez Paz , Gomez‐Jurado Gema , Gomez Tarrias Consuelo , Hidalgo Isla Margarita , Jimenez Andrés Patricia , Linares Gago Marta , Lirola‐Liebanas Ana , Mesa‐Ruiz Antonio , Muñoz‐Garach Araceli , Salazar‐Graván Susana , Estevez‐Lopez Fernando , Martín‐Matillas Miguel , Ariza‐Vega Patrocinio 
Published: February 2022

AbstractTelerehabilitation interventions administered via a smartphone may provide new feasible and effective rehabilitation options at home for patients with hip fracture. However, to date, no such interventions have been shown to be effective in the recovery key health outcomes of these patients. The present multicentre randomized controlled trial (RCT) aims to test the effect of the ActiveHip+ m‐Health system in the recovery of physical performance, functional level, quality of life, and other health‐related outcomes in both patients with hip fracture and their family caregivers. A total of 104 patients older than 65 years, with hip fracture, and their family caregivers will be randomized into the ActiveHip+ rehabilitation (N = 52) or the control group (N = 52). ActiveHip+ is a 12‐week smartphone‐based rehabilitation program conducted in Granada and Cádiz (Spain) that includes: (1) 24 sessions of physical exercise and 12 sessions of occupational therapy; (2) seven educational modules for patients and for caregivers; and (3) general recommendations in activities of daily living. The control group will receive the usual rehabilitation protocol offered by the Andalusian Public Healthcare System. The primary outcome is the patient's physical performance, while the secondary outcomes are the patient's functional level, quality of life, pain, fear of falling, fitness perception, pre‐fracture functional level, emotional status, and caregiver burden. The present project will substantially contribute to the existing knowledge by testing for the first time the efficacy and feasibility of a multidisciplinary m‐Health system in the rehabilitation of patients with hip fracture.