bluuebunny/crossref_metadata_2025_split huggingface dataset:
Time Series Single-Cell Transcriptional Atlases Reveal Cell Fate Differentiation Driven by Light in Arabidopsis Seedlings
Authors: He Hang , Deng Xing Wang , Han Xue , Zhang Yilin , Lou Zhiying , Li Jian , Gao Chunlei , Liu Yi , Li Bosheng , Pan Wenbo , Zhang Huawei 
Published: October 2022

Abstract
Light serves as the energy source as well as a signal for plant growth and development during their whole life cycle. Seedling de-etiolation is the most dramatic manifestation of light-regulated plant development processes, as massive reprogramming of the plant transcriptome occurs. Although there were organ-specific development and genome expression studies reported, there still lack a systematic analysis of cell type specific differentiation and the associated genome transcriptional regulation. Here, we obtained single-cell transcriptional atlases for etiolated, de-etiolating and light-grown Arabidopsis (Arabidopsis thaliana) whole seedlings. These atlases comprised 31,796 and 61,065 informative cells from shoot and root tissues, respectively, that were clustered and annotated as 48 different cell types. Although key transcription factors underlying photomorphogenesis such as HY5/HYH and PIF1,5 were found to be universally induced by light in all the evaluated cell types, one-third (12,447) of all the genes showed preferential spatiotemporal expression during de-etiolation. Different development occurred in respective cell types in pifq mutants, leading to photomorphogensis-like epidermal cells and skotomorphogenesis-like mesophyll cells. With the determination of comprehensive developmental trajectories, light modulation of cell fate differentiation during hook opening, guard cell specialization, and vasculature development have been demonstrated. A series of cell type-specific developmental factors modulated by light were identified, revealing strikingly different light-induced switches in their respective cell types. Our results provide information concerning the light signaling networks at the cell-type resolution, improving our understanding of how light regulates plant development at the cell-type and genome-wide levels. The learned information could serve as a valuable resource for comprehensively investigating molecular mechanism of cell development and differentiation in response to light.
Influencing Factors of Community Residents' Pro-environmental Behavior in East Dongting Lake National Nature Reserve under the Policy Intervention
Authors: Tang Xianglong , Yuan Jianqiong , Zeng Xitong 
Published: October 2022

Abstract
In the study of protected areas, the " Fences & fines " approach is increasingly becoming acknowledged as obsolete and ineffectual, and there is mounting evidence suggesting that the "Community-based conservation" approach is acquiring consideration. It is significant to identify which protection model or factors perform a definitive part in China. Taking the East Dongting Lake National Nature Reserve in China as a survey site, this paper utilizes a random sampling method to interview 431 households to investigate the relationship between legal system, ecological compensation, environmental education, community participation, concessions, livelihoods, job provision, intrinsic motivation and pro-environmental behavior. The regression results declare that intrinsic motivation and legal system are the most effective factors impacting on pro-environmental behavior; concessions has a critical adverse consequence on pro-environmental behavior; ecological compensation, community participation, environmental education, livelihoods, and job provision had insignificant positive impacts on pro-environmental behavior. Further mediating effects analysis and SEM model construction indicated that intrinsic motivation mediates between legal system and pro-environmental behavior of community residents, legal system promotes pro-environmental behavior by promoting intrinsic motivation, which is more effective than legal system promoting pro-environmental behavior directly. This demonstrates that in communal protected areas, long-term strict legal constraints can shape community residents' positive attitude towards conservation and pro-environmental behavior, and appropriate "community-based conservation" approaches can mitigate conflicts between special groups represented by hunters and protected areas. With the combination of these two approaches, the management of protected areas can be successful. This supplies a valuable real-world case for the current debate on biodiversity conservation.
Chaotic Slime Mould Inspired Hybrid Optimizer Approach for Day Ahead Generation Scheduling Problem
Authors: Bhadoria Ashutosh , Marwaha Sanjay 
Published: October 2022

Abstract
In the world of optimization algorithms, hybrid algorithms are gaining more and more popularity, in this paper, novel hybrid algorithm is proposed to solve generation scheduling problem by using Chaotic Slime mould optimization algorithm (CSMA) and seagull optimization algorithm (SOA). Generation scheduling problem is one of the most challenging problem of modern power system due to its combinatorial nature and non-linear constraints. The proposed optimizer is initially tested for various CEC2017 benchmark problems for its effective analysis and in the next stage, the proposed optimizer has been applied to solve generation scheduling problem. The proposed method works in three steps: first, the CSMA-SOA solves the unit commitment problem while ignoring the various constraints. Second, previous solutions are forced to agree with unavoidable constraints using a heuristic constraints repair mechanism; finally, the proposed method is used to establish the most cost-effective solution in giving timeframe. This study further investigate solar energy. Solar irradiance is thought to have a stochastic nature and adhere to Beta PDF. The performance of hybrid optimizers has been tested on a wide range of units. The results clearly show that the proposed optimizer performs much better than other well-known heuristics, meta-heuristics and hybrid optimizers.
UQCRFS1 serves as a biomarker of poor prognosis and promotes ovarian cancer progression
Authors: Sun Qiran , Li Jiaqi , Ding Jiashan , Li Yuan , Dong Hao , Zhan Jiao , Xiong Xiaoming , He Linsheng , Wang Jing 
Published: October 2022

Abstract

Background
UQCRFS1 is a crucial subunit of mitochondrial compound III, which mediates the mitochondrial electron transfer process. Although the gene has been reported to be highly expressed in gastric and breast cancer, the prognosis and biological functions of UQCRFS1 in ovarian cancer have not been evaluated.
Methods
UQCRFS1 mRNA and protein expression levels were determined using the Human Protein Atlas (HPA) database and Gene Expression Profiling Interactive Analysis (GEPIA). Western blotting and immunohistochemistry were used for validation. The effect of prognosis was investigated based on the cancer genome atlas (TCGA) database. The correlation with tumor-related signature was evaluated based on c-BioPortal database. Knockdown cell lines were further constructed to verify the biological effect of this gene. Total and phosphorylated levels of AKT and mTOR were analysed by western blotting.
Results
GEPIA and HAP analysis revealed that UQCRFS1 was overexpressed in ovarian cancer compared with normal tissues, which was associated with poor prognosis in ovarian cancer patients. Western blotting and immunohistochemistry also confirmed this finding. Correlation analysis showed that UQCRFS1 overexpression was positively correlated with cycle and oxidative phosphorylation signature, and negatively correlated with apoptosis and DNA damage. Functional analysis showed that UQCRFS1 knockdown resulted in attenuated ovarian cancer cell proliferation, increased G1 phase, and increased apoptosis rate. In addition, mRNA expression of DNA damage genes increased, ROS levels increased, and total AKT and mTOR levels and phosphorylation levels decreased.
Conclusions
UQCRFS1 is overexpressed in ovarian cancer and is associated with poor prognosis. UQCRFS1 affects ovarian cell survival by affecting proliferation, cell cycle, DNA damage and ROS levels, which may be attributed to the AKT/mTOR pathway.
Completion of Four or More ANC Visits Among Women of Reproductive Age in a Rural District in Uganda: A Quantitative Study on the Role of Service-Level Factors
Authors: Muhirwe Lorna Barungi 
Published: October 2022

Abstract

Uganda is one of 24 countries in the world where 70% of global maternal deaths occur. Evidence indicates that antenatal care (ANC) has both indirect and direct effects on maternal and perinatal morbidity and mortality reduction. In Uganda, the ANC attendance rate stands at 97.3% for one visit, but 59.9% for four or more visits. The aim of this quantitative study was to assess service- level factors affecting completion of ANC attendance defined by completion of four or more visits among women of reproductive age in a rural district in Uganda to contribute to design of patient-centered ANC services. Facility assessment scores on the service-level factors of interest for health facilities were obtained using a service level index tool. The relationship between the ANC completion rates of clients sampled from records at the health facilities and facility scores on service-level factors of interest were analyzed. Regression was conducted to determine the predictive relationship between ANC service availability, ANC service content, and ANC
service organization, and completion of ANC attendance. The model was statistically significant, χ2 (6) = 26.118, p ˂ 0.05, and accounted for approximately 17.3% of the variance of ANC attendance completion (R2 = .173). Completion of ANC attendance was primarily predicted by better timing of provision of ANC services, and to a lesser extent by higher levels of availability of medicines and medical supplies. This study demonstrated that service-level factors have a predictive value for completion of ANC attendance. The findings can be used to improve availability, content, and organization of ANC services with the aim of enhancing positive experiences for clients and motivating them to complete the recommended number of ANC visits.
Experimental modelling of compact Double-GasChamber Hydro-Pneumatic Strut considering gas temperature and heat transfer
Authors: Li Ruihong , Yang Fan , Lin Dezhao , Zhang Weiqiang 
Published: October 2022

Abstract
The gas relationship is one of the important inherent characteristics of the Hydro-Pneumatic Strut (HPS) system, which will affect the gas/oil pressure distribution and then system output characteristic. The ideal gas law utilized by most researchers in the modelling of HPS structure cannot well describe the gas characteristics, such as the hysteresis and other nonlinear phenomena, and then the dynamic properties of the HPS system. The compacted Double-Gas-Chamber Hydro-Pneumatic Strut (DHPS) system consists of double gas chambers combined in the strut, and then dynamic properties of the strut are much more sensitive to the gas relationship as the heat generated in the working condition is directly transferred to the gas chambers without buffer through the hydraulic media comparing to the HPS system with external gas chamber(s) arrangement. In this study, the Benedict-Webb-Rubin (BWR) equation with the heat transfer equation considering the variation of air contact area has been established and utilized to describe the gas properties of two gas chambers in the designed compact DHPS. It has been shown that the coefficient of determinations (R2) between the experimental data and model estimated result of the gas pressure in two chambers were both above 0.989. Besides, the dynamic properties of the compacted DHPS have been established based on the established gas relationship combined with the friction model, orifice damping force and inertial properties of both floating pistons. Good agreement can be found from the comparison of the dynamic properties of the designed compacted DHPS between the experimental data and the model estimation result. Through extensive numerical analysis, it has been shown that the necessity of the established gas model, the effect of orifices damping and friction forces, and slight effect of the inertial force of the floating pistons, to the dynamic properties of the compacted DHPS system.
Looking for hypoxia-fingerprint in multiple myeloma and malignant melanoma patients' transcriptomic data using a binominal distribution model
Authors: Kocemba-Pilarczyk Kinga A. , Leskiewicz Katarzyna , Trojan Sonia , Piwowar Monika 
Published: October 2022

Abstract
Background/aim:
Reduced partial oxygen pressure is a characteristic feature of many cancers. HIF-1 transcription factor, activated under hypoxic conditions, alters the gene expression profile, triggering genes, which facilitate the survival of cells in oxygen diminished environment. Importantly, the HIF-1 signaling pathway itself has been considered a potential target of anti-cancer therapy since inhibition of this pathway may significantly slow down tumor growth.
Materials and methods
Initially, using the in vitro hypoxic conditions we determined the set of hypoxia target genes forming the hypoxia signature for Malignant Melanoma and Multiple Myeloma cells. Subsequently, the expression profile of selected genes was tested on patients’ transcriptomic data sets using binominal distribution model.
Results
Based on in vitro experiment we determined the 12 hypoxia target genes forming the hypoxia signature for Malignant Melanoma and 12 hypoxia target genes for Multiple Myeloma. Importantly, 9 genes out of 12 were common for both neoplasms. The analysis of expression distribution for selected genes revealed that in Multiple Myeloma the distribution pattern follows the theoretical binominal distribution model, opposite to Malignant Melanoma, where groups of patients with high probability of active or non-active HIF-1 signaling were apparently visible.
Conclusion
Our analysis clearly indicates that the probability of HIF-1 pathway activation can be assessed using binominal distribution model for the selected genes forming the hypoxia signature. The model developed based on two studied neoplasms expeditiously verifies the subgroups with high and/or low probability of HIF-1 signaling in a given transcriptomic data set and allows for assessing the probability of hypoxia pathway activation or any other active signaling in tumors, at the level of individual patients.
Assessment of landslide hazards in Miocene Basins based on Modified Frequency Ratio and Limit Equilibrium models: A case study in Aomar Miocene basin, Bouira, Algeria
Authors: Nassim HALLAL , Hamai Lamine , Hamidatou Mouloud , Lamali Atmane , YELLES-CHAOUCHE Abdelkrim , Medjnoun Amal 
Published: October 2022

Abstract
The Neogene basins are well known for their high susceptibility to landslides. This feature is mainly favoured by the lithological nature of the filling materials and slope morphology. This study proposes a combined frequency ratio and limits equilibrium methodology to perform a regional analysis of slope stability in the Neogene basin of Aomar Ben Haroun (Bouira, Algeria). For this purpose, different work steps have been conducted. Initially, the field site selection was made according to the surface geology underlying ground motion and the extent of landslide events. Subsequently, the landslide survey was built on existing studies and on high-resolution aerial photography and satellite imagery analysis, as well as on detailed fieldwork involving mapping and compilation of triggering factors. Besides, the evaluation of the landslide susceptibility around the study area was performed using the frequency ratio (FR) in the GIS environment. Note that the geotechnical characterization of each formation was determined from the physical and mechanical parameters of recent and old geotechnical studies carried out in the region. Moreover, the determination of the​​ landslide-prone areas with a characteristic stability factor for each slope was then computed using a two-dimensional limit equilibrium model. Thanks to the provided results, the classes of slopes that are characterized by parting surfaces susceptible to landslides are clearly defined. The result maps of the Aomar Miocene basin were categorized into low, moderate and high susceptibility classes. We have then been able to validate the obtained results by each used method. In the case of the inventory and susceptibility maps, we have also compared the two results based on the results of the area under the curve (AUC). For the frequency ratio (FR) and Limit Equilibrium (LE) models, the AUC are (83.23%) and (89.25%) respectively, which are indicating good and very good accuracy in the identification of landslide susceptibility zones of a basin and can be used in land use planning. In conclusion, this methodology highlights the fact that the landslides susceptibility map, which is obtained using the limit equilibrium model, was significantly more reliable and consistent than the susceptibility map completed by the frequency ratio model after field verification.
Magnitude of common mental disorders and associated factors among pregnant women attending Antenatal Care visits in North Wollo Public Health Facilities, Northwest Ethiopia
Authors: Dessie Amanuel Addisu , Tenaw Lebeza Alemu , Arage Mulugeta Wodaje , Demeke Solomon Moges , Melese Amare Alemu , Bantie Getasew Mulat 
Published: October 2022

Abstract
Introduction: Mental health disorders during pregnancy are of public health concern because of the implications for the mother and child's health. Mental health issues during pregnancy must, therefore, be diagnosed and addressed. And interventions to improve maternal mental health during pregnancy are critical preventive strategies against adverse health and developmental outcomes in newborns and children. Therefore, this study aimed to assess the prevalence of common maternal mental disorders and associated factors among pregnant women in North Wollo, Northwest Ethiopia.
Objective: The study aimed to assess the magnitude of common mental disorders and associated factors among pregnant women in North Wollo, 2022
Method: Institution-based cross-sectional study was conducted in North Wollo zone public health facilities from February to April 2022. A total of 777 samples were selected using a multistage sampling technique. Women were assessed for the risk of common mental disorders using a pretested, structured, self-reporting questionnaire (SRQ-20). The collected data were entered into Epi-data version 3.1 software and analyzed using R version 4.0 software. Descriptive statistics were computed. A simple logistic regression model was fit to identify the association and strength of exploratory variables and common mental disorders at a 95% confidence interval and p-value < 0.05.
Results: The study revealed that the prevalence of common mental disorders during pregnancy was 18.1% (95%CI: 15.5, 21.0). Educational status of participants (AOR=0.17, 95% CI: 0.06, 0.48), husband educational status (AOR=11.13, 95%: 4.18, 29.66), unplanned pregnancy (AOR=2.54, 95% CI: 1.26, 5.09), self-reported complication on the current pregnancy (AOR=0.11, 95% CI: 0.05, 0.21), self-reported complication during the previous delivery (AOR=3.38, 95% CI: 1.39, 8.18), nutritional status (AOR=2.19, 95%: 1.26, 3.81), psychosocial risk (AOR=20.55, 95% CI: 9.69, 43.59), legal problem (AOR=2.06, 95%: 1.12, 3.79) and relationship problem (AOR=7.22, 95% CI: 3.59, 14.53) were showed statistical significant association.
Conclusions: The prevalence of common mental disorders among pregnant women was high. Educational status of the participants and their spouse, unplanned pregnancy, self-reported complication during current pregnancy and previous delivery, nutritional status, psychosocial risk, and legal and relationship problem were the main determinants of CMD.
Clinical characteristics and risk factors of organ failure and death in necrotizing pancreatitis
Authors: Yu Liqing , Xie Fengwen , He Cong , Li Nianshuang , He Wenhua , Lu Nonghua , Yu Bingjun 
Published: October 2022

Abstract
Background: Organ failure (OF) and death are considered the most significant adverse outcome in necrotizing pancreatitis (NP). However, there are few NP-related studies describing the clinical traits of OF and aggravated outcomes.
Purpose: Our objective was not only to address the risk factors of OF and death in NP patients, but further identify simple parameters that can discriminate high-risk patients of NP to improve outcomes.
Methods: We performed a study of 432 NP patients from May 2017 to December 2021. All patients with NP were followed for 36 months. The primary end point was risk factors of OF and death in NP patients. The risk factors that were evaluated by logistic regression analysis.
Results: Patients of NP with OF or death were generally older, higher APACHE II score, longer hospital stay, longer ICU stay, as well as a higher incidence of severe acute pancreatitis (SAP), shock and pancreatic necrosis. Independent risk factors related to OF included BMI, APACHE II score and SAP (P < 0.05). Age, shock and APACHE II score (P < 0.05) were the most significant factors correlated with the risk of death in NP patients. Notably, increased mortality was linked to the number of failed organs.
Conclusions: NP is a potentially fatal disease with a longer hospital or ICU stay. Our study reported that the incidence of OF and death in NP patients are 69.9% and 10.2%, respectively. BMI, SAP, APACHE II score, age and shock could be the risk factors of OF and death in NP patients. Clinicians should focus on these factors for early diagnosis and appropriate therapy.
Investigation of thermophysical and physicochemical characteristics of Al 2 O 3 -SiO 2 - CaO - Na 3 AlF 6 flux for SMAW electrode coating
Authors: Gupta Alok , Singh Jaiveer , Chhibber Rahul 
Published: October 2022

Abstract
This study investigated the thermophysical, physicochemical, and electrical characteristics of electrode coatings developed for application in the weld joint of nuclear power plants (NPPs). The extreme vertices design technique has been used in this work to develop the Al2O3-SiO2-CaO-Na3AlF6-based SMAW electrodes. Twenty-six electrode coating compositions were formulated, and flux coatings were milled into a fine powder for further characterization. XRD technique was utilized to investigate the structure and phases of the coating composition, and the FTIR analysis technique was employed to determine the nature of bonds. The coating's thermal characteristics, including conductivity, diffusivity, and specific heat, were evaluated using hot disk equipment. Enthalpy change and thermal stability of flux coating were determined using the TGA technique. The precision LCR instrument was used further to examine the electrical characteristics of the flux coatings. In addition, a regression model has been developed for each coating property using statistical analysis and investigated a correlation between the properties and mineral interactions. Results reveal that the individual elements and their binary and tertiary interaction significantly influence the physicochemical, thermophysical, and electrical properties of the flux coatings.