bluuebunny/crossref_metadata_2025_split huggingface dataset:
A highly immunogenic vaccine platform against encapsulated pathogens using chimeric probiotic Escherichia coli membrane vesicles
Authors: Nakao Ryoma , Kobayashi Hirotaka , Iwabuchi Yusuke , Kawahara Kazuyoshi , Hirayama Satoru , Ramstedt Madeleine , Sasaki Yuki , Kataoka Michiyo , Akeda Yukihiro , Ohnishi Makoto 
Published: November 2022

AbstractVaccines against infectious diseases should elicit potent and long-lasting immunity, ideally even in those with age-related decline in immune response. Here we report a rational polysaccharide vaccine platform using probiotic Escherichia coli-derived membrane vesicles (MVs). First, we constructed a probiotic E. coli clone harboring the genetic locus responsible for biogenesis of serotype 14 pneumococcal capsular polysaccharides (CPS14) as a model antigen. CPS14 was found to be polymerized and mainly localized on the outer membrane of the E. coli cells. The glycine-induced MVs displayed the exogenous CPS14 at high density on the outermost surface, on which the CPS14 moiety was covalently tethered to a lipid A-core oligosaccharide anchor. In in vivo immunization experiments, CPS14+MVs, but not a mixture of free CPS14 and empty MVs, strongly elicited IgG class-switch recombination with a Th1/Th2-balanced IgG subclass distribution without any adjuvant. In addition, CPS14+MVs were structurally stable with heat treatment and immunization with the heat-treated MVs-elicited CPS14-specific antibody responses in mouse serum to levels comparable to those of non-treated CPS14+MVs. Notably, the immunogenicity of CPS14+MVs was significantly stronger than those of two currently licensed vaccines against pneumococci. The CPS14+MV-elicited humoral immune responses persisted for 1 year in both blood and lung. Furthermore, the CPS14+MV vaccine was widely efficacious in mice of different ages. Even in aged mice, vaccination resulted in robust production of CPS14-specific IgG that bound to the pneumococcal cell surface. Taken together, the present probiotic E. coli MVs-based vaccine platform offers a promising, generalizable solution against encapsulated pathogens.
Deglacial increase of seasonal temperature variability in the tropical ocean
Authors: Wörmer Lars , Wendt Jenny , Boehman Brenna , Haug Gerald H. , Hinrichs Kai-Uwe 
Published: November 2022

AbstractThe relatively stable Holocene climate was preceded by a pronounced event of abrupt warming in the Northern Hemisphere, the termination of the Younger Dryas (YD) cold period1,2. Although this transition has been intensively studied, its imprint on low-latitude ocean temperature is still controversial and its effects on sub-annual to decadal climate variability remain poorly understood1,3,4. Sea surface temperature (SST) variability at these timescales in the tropical Atlantic is expected to intensify under current and future global warming and has considerable consequences for environmental conditions in Africa and South America, and for tropical Pacific climate5–8. Here we present a 100-µm-resolution record obtained by mass spectrometry imaging (MSI) of long-chain alkenones in sediments from the Cariaco Basin9–11 and find that annually averaged SST remained stable during the transition into the Holocene. However, seasonality increased more than twofold and approached modern values of 1.6 °C, probably driven by the position and/or annual range of the Intertropical Convergence Zone (ITCZ). We further observe that interannual variability intensified during the early Holocene. Our results demonstrate that sub-decadal-scale SST variability in the tropical Atlantic is sensitive to abrupt changes in climate background, such as those witnessed during the most recent glacial to interglacial transition.
Global hotspots of salt marsh change and carbon emissions
Authors: Campbell Anthony D. , Fatoyinbo Lola , Goldberg Liza , Lagomasino David 
Published: November 2022

AbstractSalt marshes provide ecosystem services such as carbon sequestration1, coastal protection2, sea-level-rise (SLR) adaptation3 and recreation4. SLR5, storm events6, drainage7 and mangrove encroachment8 are known drivers of salt marsh loss. However, the global magnitude and location of changes in salt marsh extent remains uncertain. Here we conduct a global and systematic change analysis of Landsat satellite imagery from the years 2000–2019 to quantify the loss, gain and recovery of salt marsh ecosystems and then estimate the impact of these changes on blue carbon stocks. We show a net salt marsh loss globally, equivalent to an area double the size of Singapore (719 km2), with a loss rate of 0.28% year−1 from 2000 to 2019. Net global losses resulted in 16.3 (0.4–33.2, 90% confidence interval) Tg CO2e year−1 emissions from 2000 to 2019 and a 0.045 (−0.14–0.115) Tg CO2e year−1 reduction of carbon burial. Russia and the USA accounted for 64% of salt marsh losses, driven by hurricanes and coastal erosion. Our findings highlight the vulnerability of salt marsh systems to climatic changes such as SLR and intensification of storms and cyclones.
Ras drives malignancy through stem cell crosstalk with the microenvironment
Authors: Yuan Shaopeng , Stewart Katherine S. , Yang Yihao , Abdusselamoglu Merve Deniz , Parigi S. Martina , Feinberg Tamar Y. , Tumaneng Karen , Yang Hanseul , Levorse John M. , Polak Lisa , Ng David , Fuchs Elaine 
Published: November 2022

AbstractSquamous cell carcinomas are triggered by marked elevation of RAS–MAPK signalling and progression from benign papilloma to invasive malignancy1–4. At tumour–stromal interfaces, a subset of tumour-initiating progenitors, the cancer stem cells, obtain increased resistance to chemotherapy and immunotherapy along this pathway5,6. The distribution and changes in cancer stem cells during progression from a benign state to invasive squamous cell carcinoma remain unclear. Here we show in mice that, after oncogenic RAS activation, cancer stem cells rewire their gene expression program and trigger self-propelling, aberrant signalling crosstalk with their tissue microenvironment that drives their malignant progression. The non-genetic, dynamic cascade of intercellular exchanges involves downstream pathways that are often mutated in advanced metastatic squamous cell carcinomas with high mutational burden7. Coupling our clonal skin HRASG12V mouse model with single-cell transcriptomics, chromatin landscaping, lentiviral reporters and lineage tracing, we show that aberrant crosstalk between cancer stem cells and their microenvironment triggers angiogenesis and TGFβ signalling, creating conditions that are conducive for hijacking leptin and leptin receptor signalling, which in turn launches downstream phosphoinositide 3-kinase (PI3K)–AKT–mTOR signalling during the benign-to-malignant transition. By functionally examining each step in this pathway, we reveal how dynamic temporal crosstalk with the microenvironment orchestrated by the stem cells profoundly fuels this path to malignancy. These insights suggest broad implications for cancer therapeutics.
Social trauma engages lateral septum circuitry to occlude social reward
Authors: Li Long , Durand-de Cuttoli Romain , Aubry Antonio V. , Burnett C. Joseph , Cathomas Flurin , Parise Lyonna F. , Chan Kenny L. , Morel Carole , Yuan Chongzhen , Shimo Yusuke , Lin Hsiao-yun , Wang Jun , Russo Scott J. 
Published: November 2022

AbstractIn humans, traumatic social experiences can contribute to psychiatric disorders1. It is suggested that social trauma impairs brain reward function such that social behaviour is no longer rewarding, leading to severe social avoidance2,3. In rodents, the chronic social defeat stress (CSDS) model has been used to understand the neurobiology underlying stress susceptibility versus resilience following social trauma, yet little is known regarding its impact on social reward4,5. Here we show that, following CSDS, a subset of male and female mice, termed susceptible (SUS), avoid social interaction with non-aggressive, same-sex juvenile C57BL/6J mice and do not develop context-dependent social reward following encounters with them. Non-social stressors have no effect on social reward in either sex. Next, using whole-brain Fos mapping, in vivo Ca2+ imaging and whole-cell recordings, we identified a population of stress/threat-responsive lateral septum neurotensin (NTLS) neurons that are activated by juvenile social interactions only in SUS mice, but not in resilient or unstressed control mice. Optogenetic or chemogenetic manipulation of NTLS neurons and their downstream connections modulates social interaction and social reward. Together, these data suggest that previously rewarding social targets are possibly perceived as social threats in SUS mice, resulting from hyperactive NTLS neurons that occlude social reward processing.
A database of water chemistry in eastern Siberian rivers
Authors: Liu Shiqi , Wang Ping , Huang Qiwei , Gabysheva Olga I. , Li Zehong , Zhang Jialing , Kazak Ekaterina S. , Liu Yu , Bazarzhapov Tcogto Zh. , Shpakova Raisa N. , Gabyshev Viktor A. , Pozdniakov Sergey P. , Frolova Natalia L. 
Published: November 2022

AbstractPermafrost degradation leads to considerable changes in river ecosystems. The Eastern Siberian River Chemistry (ESRC) database was constructed to create a spatially extensive river chemistry database to assess climate warming-induced changes in freshwater systems in permafrost-dominated eastern Siberia. The database includes 9487 major ion (Na+, K+, Ca2+, Mg2+, Cl−, SO42− and HCO3−) data of chemical results from 1434 water samples collected mainly in six large river basins in eastern Siberia spanning 1940–2019. Data were obtained from public databases, scientific literature in English and Russian, and researchers and were formatted with a consistent table structure. The database is transparent and reproducible. Climate variable (air temperature and precipitation) data, discharge data, trace element concentration data, and isotope data at the basin and subbasin scales are also provided. This database enhances knowledge about the water chemistry of the permafrost region, especially in eastern Siberia, where data are scarce. The database will be useful to those assessing spatiotemporal changes in river water chemistry associated with permafrost degradation or other environmental stressors in a warmer climate.
Numerical study of lateral coolant jet on heat reduction over nose cone with double-aerodome at hypersonic flow
Authors: Ghanbari Mehdi , Maddah Soroush , Alinejad Javad 
Published: November 2022

AbstractOne of the main challenges in designing a supersonic forebody is thermal protection. The application of the mechanical spike mounted at the nose considerably decreases the heat load on the main body. In this investigation, the hybrid technique of mechanical spike and coolant injection are examined to reduce the thermal load on the nose cone in the supersonic air stream. A three-dimensional model of a double aerodisked spike with different cooling systems is provided to find the efficient cooling injection system for reducing the heat load on the nose cone. Computational studies have been done on investigating a cooling mechanism in the proposed injection systems. This study has tried to present valuable information on flow features and shock interaction nearby the nose. The influence of different coolant gas on the thermal performance of the proposed configurations is comprehensively explained. Our results indicate that the cooling performance of single carbon dioxide is 85% more than helium jet in lateral injection. According to our findings, the cooling performance of lateral multi-jets is 90% more than opposing ones.
Fatigue, sleep quality and mental health symptoms in Brazilian women during the COVID-19 pandemic: longitudinal study
Authors: dos Santos Gabriel Bernardi , Beleza Ana Carolina Sartorato , Sato Tatiana de Oliveira , Carvalho Cristiano , Serrão Paula Regina Mendes da Silva 
Published: November 2022

AbstractTo assess the impact of the COVID-19 pandemic on the variables of sleep quality, fatigue, anxiety, and depression in healthy Brazilian women. Longitudinal observational study conducted through an online questionnaire with women in 2020 and 2021. The Pittsburgh Sleep Quality Index, the Fatigue Severity Scale and the Hospital Anxiety and Depression Scale were used. The data were analyzed descriptively and the comparison between the data obtained in the first and second evaluation was performed using the McNemar test. A logistic regression was applied to test the association between the variables that showed a significant difference. A total of 235 women responded to the questionnaires. There was a significant increase in fatigue between the two moments (p < 0.05). In the first assessment, depression (OR: 2.39; 95% CI: 1.14–4.99), anxiety (OR: 2.68; 95% CI: 1.37–5.22) and sleep quality (OR: 4.01; 95% CI: 1.71–9.67) were associated with fatigue. In the second assessment, depression (OR: 2.93; 95% CI: 1.19–7.18) and anxiety (OR: 2.69; 95% CI: 1.27–5.71) were associated with fatigue. There was an impact on biopsychosocial aspects during the COVID-19 pandemic, with worsening of fatigue symptoms within a 6-month interval. In addition, fatigue was associated with symptoms of depression and anxiety, and worse sleep quality in the first year of the pandemic, remaining associated with symptoms of depression and anxiety in the second year of the pandemic in the country.
Self-reported changes in sleep patterns and behavior in children and adolescents during COVID-19
Authors: Bothe Kathrin , Schabus Manuel , Eigl Esther-Sevil , Kerbl Reinhold , Hoedlmoser Kerstin 
Published: November 2022

AbstractThe COVID-19 pandemic and lockdowns worldwide forced children and adolescents to change and adapt their lives to an unprecedented situation. Using an online survey, we investigated whether they showed changes in sleep quality and other related factors due to this event. Between February 21st, 2021 and April 19th, 2021, a total of 2,290 Austrian children and adolescents (6–18 years) reported their sleep habits and quality of sleep as well as physical activity, daylight exposure and usage of media devices during and, retrospectively, before the pandemic. Results showed an overall delay of sleep and wake times. Almost twice as many respondents reported having sleeping problems during the pandemic as compared to before, with insomnia, nightmares and daytime sleepiness being the most prevalent problems. Furthermore, sleeping problems and poor quality of sleep correlated positively with COVID-19 related anxiety. Lastly, results showed a change from regular to irregular bedtimes during COVID-19, higher napping rates, a strong to very strong decrease in physical activity and daylight exposure, as well as a high to very high increase in media consumption. We conclude that the increase in sleeping problems in children and adolescent during COVID-19 is concerning. Thus, health promoting measures and programs should be implemented and enforced.
Serum vitamin D levels and acute kidney injury: a systemic review and meta-analysis
Authors: Zhang Huanran , Jiang Yan , Shi Nan , Lu Yuan-Qiang 
Published: November 2022

AbstractNumerous researches have evaluated the prevalence and clinical outcome of vitamin D deficiency in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD). But the quantitative vitamin D status in acute kidney injury (AKI) patients and its relationship with prognosis remains controversial. We conducted this systemic review and meta-analysis to assess the quantitative difference of vitamin D status, including serum 25(OH) D and 1,25(OH)2D levels, between AKI patients and non-AKI controls, and further explore whether vitamin D status can be clearly correlated with the mortality of AKI. Major databases, including PubMed, Web of Science and EBSCO, were searched until 1st September 2021. All published observational studies related to vitamin D and AKI According to predefined inclusion and exclusion criteria were extracted. Meta-analyses were performed using Review Manager 5.3.5. Four studies including five cohorts were included with a total of 413 patients. The serum 25(OH)D levels showed no statistically significant difference in AKI patients and non-AKI controls. On the other hand, the serum 1,25(OH)2D levels were significant lower in AKI patients than in non-AKI controls (MD =  − 17.79, 95% CI =  − 32.73 to − 2.85, P = 0.02). As for the relationship between serum vitamin D status and AKI patients’ mortality, we were unable to give a consistent conclusion based on current limited and conflict study results. Our meta-analysis suggested that serum 1,25(OH)2D levels, rather than 25(OH)D, is significantly lower in AKI patients. The relationship between vitamin D status and clinical outcome of AKI remains controversial based on current evidence. Future comprehensive studies are required to confirm these relations and to elucidate potential mechanisms.
Diagnostic performance of attenuated total reflection Fourier-transform infrared spectroscopy for detecting COVID-19 from routine nasopharyngeal swab samples
Authors: Heino Helinä , Rieppo Lassi , Männistö Tuija , Sillanpää Mikko J. , Mäntynen Vesa , Saarakkala Simo 
Published: November 2022

AbstractAttenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy coupled with machine learning-based partial least squares discriminant analysis (PLS-DA) was applied to study if severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could be detected from nasopharyngeal swab samples originally collected for polymerase chain reaction (PCR) analysis. Our retrospective study included 558 positive and 558 negative samples collected from Northern Finland. Overall, we found moderate diagnostic performance for ATR-FTIR when PCR analysis was used as the gold standard: the average area under the receiver operating characteristics curve (AUROC) was 0.67–0.68 (min. 0.65, max. 0.69) with 20, 10 and 5 k-fold cross validations. Mean accuracy, sensitivity and specificity was 0.62–0.63 (min. 0.60, max. 0.65), 0.61 (min. 0.58, max. 0.65) and 0.64 (min. 0.59, max. 0.67) with 20, 10 and 5 k-fold cross validations. As a conclusion, our study with relatively large sample set clearly indicate that measured ATR-FTIR spectrum contains specific information for SARS-CoV-2 infection (P < 0.001 for AUROC in label permutation test). However, the diagnostic performance of ATR-FTIR remained only moderate, potentially due to low concentration of viral particles in the transport medium. Further studies are needed before ATR-FTIR can be recommended for fast screening of SARS-CoV-2 from nasopharyngeal swab samples.
Integrated taxonomy reveals new threatened freshwater mussels (Bivalvia: Hyriidae: Westralunio) from southwestern Australia
Authors: Klunzinger Michael W. , Whisson Corey , Zieritz Alexandra , Benson Justin A. , Stewart Barbara A. , Kirkendale Lisa 
Published: November 2022

AbstractThe freshwater musselWestralunio carteri(Iredale, 1934) has long been considered the soleWestraluniospecies in Australia, limited to the Southwest and listed as vulnerable on the IUCN Red List and under Australian legislation. Here, we used species delimitation models based on COI mtDNA sequences to confirm existence of three evolutionarily significant units (ESUs) within this taxon and conducted morphometric analyses to investigate whether shell shape differed significantly among these ESUs. “W. carteri” I was found to be significantly larger and more elongated than “W. carteri” II and “W. carteri” II + III combined, but not different from “W. carteri” III alone. We recognise and redescribe “W. carteri” I asWestralunio carteri(Iredale, 1934) from western coastal drainages and describe “W. carteri” II and “W. carteri” III asWestralunio inbisisp. nov. from southern and lower southwestern drainages. Two subspecies are further delineated: “W. carteri” II is formally described asWestralunio inbisi inbisisubsp. nov. from southern coastal drainages, and “W. carteri” III asWestralunio inbisi meridiemussubsp. nov. from the southwestern corner. Because this study profoundly compresses the range ofWestralunio carterinorthward and introduces additional southern and southwestern taxa with restricted distributions, new threatened species nominations are necessary.
Green synthesis of zinc oxide nanoparticles using Sea Lavender (Limonium pruinosum L. Chaz.) extract: characterization, evaluation of anti-skin cancer, antimicrobial and antioxidant potentials
Authors: Naiel Bassant , Fawzy Manal , Halmy Marwa Waseem A. , Mahmoud Alaa El Din 
Published: November 2022

AbstractIn the present study, a green, sustainable, simple and low-cost method was adopted for the synthesis of ZnO NPs, for the first time, using the aqueous extract of sea lavender, Limonium pruinosum (L.) Chaz., as a reducing, capping, and stabilizing agent. The obtained ZnO NPs were characterized using ultraviolet–visible spectroscopy (UV–VIS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDX), transmission electron microscopy (TEM), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). The UV–Vis spectra of the green synthesized ZnO NPs showed a strong absorption peak at about 370 nm. Both electron microscopy and XRD confirmed the hexagonal/cubic crystalline structure of ZnO NPs with an average size ~ 41 nm. It is worth noting that the cytotoxic effect of the ZnO NPs on the investigated cancer cells is dose-dependent. The IC50 of skin cancer was obtained at 409.7 µg/ml ZnO NPs. Also, the phyto-synthesized nanoparticles exhibited potent antibacterial and antifungal activity particularly against Gram negative bacteria Escherichia coli (ATCC 8739) and the pathogenic fungus Candida albicans (ATCC 10221). Furthermore, they showed considerable antioxidant potential. Thus, making them a promising biocompatible candidate for pharmacological and therapeutic applications.
Regulatory T cells in psoriatic arthritis: an IL-17A-producing, Foxp3intCD161 + RORγt + ICOS + phenotype, that associates with the presence of ADAMTSL5 autoantibodies
Authors: Pouw Juliëtte N. , Olde Nordkamp Michel A. M. , van Kempen Tessa , Concepcion Arno N. , van Laar Jacob M. , van Wijk Femke , Spierings Julia , Leijten Emmerik F. A. , Boes Marianne 
Published: November 2022

AbstractIn psoriatic arthritis (PsA), predisposing class I HLA alleles, the presence of synovial clonally proliferated CD8 + T cells and autoantibodies all point towards the loss of immune tolerance. However, the key mechanisms that lead to immune dysregulation are not fully understood. In other types of inflammatory arthritis, T regulatory cell (Treg) dysfunction and plasticity at sites of inflammation were suggested to negatively affect peripheral tolerance. We here addressed if Treg variances associate with psoriatic disease. We collected clinical data, sera and peripheral blood mononuclear cells from 13 healthy controls, 21 psoriasis and 21 PsA patients. In addition, we obtained synovial fluid mononuclear cells from 6 PsA patients. We studied characteristics of CD4 + CD25 + CD127loFoxp3 + Tregs by flow cytometry and used ELISA to quantify antibodies against ADAMTSL5, a recently discovered autoantigen in psoriatic disease. In comparison with their circulating counterparts, Tregs from inflamed joints express increased levels of ICOS, CTLA-4 and TIGIT. Furthermore, synovial fluid-derived Tregs have a distinct phenotype, characterized by IL-17A production and upregulation of CD161 and RORγt. We identified a subset of Tregs with intermediate Foxp3 expression as the major cytokine producer. Furthermore, ICOS + Tregs associate with PsA disease activity as measured by PASDAS. Lastly, we observed that presence of the Foxp3int Tregs associates with an increased abundance of anti-ADAMTSL5 autoantibodies. Tregs derived from the inflammatory environment of inflamed PsA joints exhibit a distinct phenotype, which associates with loss of peripheral immune tolerance in psoriatic disease.