bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytokine changes in cerebrospinal fluid and plasma after emergency orthopaedic surgery
Authors: Fertleman Michael , Pereira Christopher , Dani Melanie , Harris Benjamin H. L. , Di Giovannantonio Matteo , Taylor-Robinson Simon D. 
Published: February 2022

AbstractNeuroinflammation after surgery and its contribution to peri-operative neurocognitive disorders (PND) is not well understood. Studying the association between central and peripheral cytokines and neuroinflammation is a prelude to the development of treatments for PND. Here, we investigate the hypotheses that there is a greater cytokine response in cerebrospinal fluid (CSF) than plasma after orthopaedic surgery, and that plasma cytokine levels are directly related to CSF cytokine levels, indicating that plasma cytokine levels may have potential as biomarkers of neuroinflammation. Patients admitted with a fractured neck of femur were invited to participate in this study. Participants had a spinal catheter inserted just prior to induction of anaesthesia. Samples of blood and CSF were taken before, immediately after, and on the first day following emergency surgery. The catheter was then removed. Samples were analysed for the presence of ten cytokines by immunoassay. A spinal catheter was successfully inserted in 11 participants during the 18-month study period. Five plasma cytokines (IL-4, IL-6, IL-10, IL-12p70 and IL-13) rose significantly following surgery, whereas all ten CSF cytokines rose significantly (IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IFN-γ and TNF-α) (adjusted-p < 0.05). Central (CSF) cytokine levels were consistently higher than their peripheral (plasma) counterparts after surgery, with some patients having a particularly marked neuroinflammatory response. The greatest increases occurred in IL-8 in CSF and IL-6 in plasma. There were significant, strong positive correlations between several of the measured cytokines in the CSF after surgery, but far fewer in plasma. There was no significant correlation between cytokine levels in the plasma and CSF at each of the three time points. To our knowledge, this is the first study to analyse paired samples of plasma and CSF for cytokine levels before and after emergency orthopaedic surgery. This study demonstrates that following surgery for a fractured neck of femur, there is a far greater rise in cytokines in the CSF compared to plasma. The lack of correlation between peripheral and central cytokines suggests measurement of peripheral cytokines are not necessarily related to which patients may have a large neuroinflammatory response.
Ocular anterior segment and corneal parameters evaluation in celiac disease
Authors: De Bernardo Maddalena , Vitiello Livio , Gagliardi Mario , Capasso Luigi , Rosa Nicola , Ciacci Carolina 
Published: February 2022

AbstractThis observational case–control study evaluated the anterior ocular segment parameters of patients with celiac disease with a Scheimpflug imaging system and compared them with those of a healthy controls group, highlighting potential differences related to the underlying pathogenetic mechanisms of the disease. Seventy celiac patients and 70 healthy subjects were assessed with a comprehensive ophthalmological evaluation, including clinical history, Snellen best-corrected visual acuity, axial length (AL) measurements with IOLMaster, and anterior segment tomographic evaluation with Pentacam HR. The measurements of all keratometry values, astigmatism, steep axis, anterior and posterior Q value (asphericity), pupil diameter, pupil center, corneal apex, the thinnest point, corneal volume, anterior chamber depth from the epithelium, anterior chamber depth from endothelium, anterior chamber volume, and iridocorneal angle were also appraised. The two study groups were comparable and similar for gender, age, and AL, with no statistically significant differences regarding all analyzed tomographic parameters. Thus, ocular anterior segment parameters of celiac patients are not significantly different from those of healthy subjects, suggesting no underlying pathogenetic implications of celiac disease affecting the assessed structures. Nevertheless, a routine ophthalmological examination for all celiac patients should be recommended throughout their lifetimes due to the potential ocular manifestations of the disease.
Current status of delirium assessment tools in the intensive care unit: a prospective multicenter observational survey
Authors: Ishii Kenzo , Kuroda Kosuke , Tokura Chika , Michida Masaaki , Sugimoto Kentaro , Sato Tetsufumi , Ishikawa Tomoki , Hagioka Shingo , Manabe Nobuki , Kurasako Toshiaki , Goto Takashi , Kimura Masakazu , Sunami Kazuharu , Inoue Kazuyoshi , Tsukiji Takashi , Yasukawa Takeshi , Nogami Satoshi , Tsukioki Mitsunori , Okabe Daisuke , Tanino Masaaki , Morimatsu Hiroshi 
Published: February 2022

AbstractDelirium is a critical challenge in the intensive care unit (ICU) or high care unit (HCU) setting and is associated with poor outcomes. There is not much literature on how many patients in this setting are assessed for delirium and what tools are used. This study investigated the status of delirium assessment tools of patients in the ICU/HCU. We conducted a multicenter prospective observational study among 20 institutions. Data for patients who were admitted to and discharged from the ICU/HCU during a 1-month study period were collected from each institution using a survey sheet. The primary outcome was the usage rate of delirium assessment tools on an institution- and patient-basis. Secondary outcomes were the delirium prevalence assessed by each institution’s assessment tool, comparison of delirium prevalence between delirium assessment tools, delirium prevalence at the end of ICH/HCU stay, and the relationship between potential factors related to delirium and the development of delirium. Result showed that 95% of institutions used the Intensive Care Delirium Screening Checklist (ICDSC) or the Confusion Assessment Method for the ICU (CAM-ICU) to assess delirium in their ICU/HCU, and the remaining one used another assessment scale. The usage rate (at least once during the ICU/HCU stay) of the ICDSC and the CAM-ICU among individual patients were 64.5% and 25.1%, and only 8.2% of enrolled patients were not assessed by any delirium assessment tool. The prevalence of delirium during ICU/HCU stay was 17.9%, and the prevalence of delirium at the end of the ICU/HCU stay was 5.9%. In conclusion, all institutions used delirium assessment tools in the ICU/HCU, and most patients received delirium assessment. The prevalence of delirium was 17.9%, and two-thirds of patients had recovered at discharge from ICU/HCU.Trial registration number: UMIN000037834.
Primary glia cells from bank vole propagate multiple rodent-adapted scrapie prions
Authors: Schwenke Karla A. , Wälzlein Joo-Hee , Bauer Agnieszka , Thomzig Achim , Beekes Michael 
Published: February 2022

AbstractSince the beginning prion research has been largely dependent on animal models for deciphering the disease, drug development or prion detection and quantification. Thereby, ethical as well as cost and labour-saving aspects call for alternatives in vitro. Cell models can replace or at least complement animal studies, but their number is still limited and the application usually restricted to certain strains and host species due to often strong transmission barriers. Bank voles promise to be an exception as they or materials prepared from them are uniquely susceptible to prions from various species in vivo, in vitro and in cell-free applications. Here we present a mainly astrocyte-based primary glia cell assay from bank vole, which is infectible with scrapie strains from bank vole, mouse and hamster. Stable propagation of bank vole-adapted RML, murine 22L and RML, and hamster 263K scrapie is detectable from 20 or 30 days post exposure onwards. Thereby, the infected bank vole glia cells show similar or even faster prion propagation than likewise infected glia cells of the corresponding murine or hamster hosts. We propose that our bank vole glia cell assay could be a versatile tool for studying and comparing multiple prion strains with different species backgrounds combined in one cell assay.
A promising deep learning-assistive algorithm for histopathological screening of colorectal cancer
Authors: Ho Cowan , Zhao Zitong , Chen Xiu Fen , Sauer Jan , Saraf Sahil Ajit , Jialdasani Rajasa , Taghipour Kaveh , Sathe Aneesh , Khor Li-Yan , Lim Kiat-Hon , Leow Wei-Qiang 
Published: February 2022

AbstractColorectal cancer is one of the most common cancers worldwide, accounting for an annual estimated 1.8 million incident cases. With the increasing number of colonoscopies being performed, colorectal biopsies make up a large proportion of any histopathology laboratory workload. We trained and validated a unique artificial intelligence (AI) deep learning model as an assistive tool to screen for colonic malignancies in colorectal specimens, in order to improve cancer detection and classification; enabling busy pathologists to focus on higher order decision-making tasks. The study cohort consists of Whole Slide Images (WSI) obtained from 294 colorectal specimens. Qritive’s unique composite algorithm comprises both a deep learning model based on a Faster Region Based Convolutional Neural Network (Faster-RCNN) architecture for instance segmentation with a ResNet-101 feature extraction backbone that provides glandular segmentation, and a classical machine learning classifier. The initial training used pathologists’ annotations on a cohort of 66,191 image tiles extracted from 39 WSIs. A subsequent application of a classical machine learning-based slide classifier sorted the WSIs into ‘low risk’ (benign, inflammation) and ‘high risk’ (dysplasia, malignancy) categories. We further trained the composite AI-model’s performance on a larger cohort of 105 resections WSIs and then validated our findings on a cohort of 150 biopsies WSIs against the classifications of two independently blinded pathologists. We evaluated the area under the receiver-operator characteristic curve (AUC) and other performance metrics. The AI model achieved an AUC of 0.917 in the validation cohort, with excellent sensitivity (97.4%) in detection of high risk features of dysplasia and malignancy. We demonstrate an unique composite AI-model incorporating both a glandular segmentation deep learning model and a classical machine learning classifier, with excellent sensitivity in picking up high risk colorectal features. As such, AI plays a role as a potential screening tool in assisting busy pathologists by outlining the dysplastic and malignant glands.
Assessment of the ameliorative effect of curcumin on pendimethalin-induced genetic and biochemical toxicity
Authors: Acar Ali , Singh Divya , Srivastava Akhileshwar Kumar 
Published: February 2022

AbstractThe present study aimed to assess the toxic effects of pendimethalin herbicide and protective role of curcumin using the Allium test on cytological, biochemical and physiological parameters. The effective concentration (EC50) of pendimethalin was determined at 12 mg/L by the root growth inhibition test as the concentration reducing the root length by 50%. The roots of Allium cepa L. was treated with tap water (group I), 5 mg/L curcumin (group II), 10 mg/L curcumin (group III), 12 mg/L pendimethalin (group IV), 12 mg/L pendimethalin + 5 mg/L curcumin (group V) and 12 mg/L pendimethalin + 10 mg/L curcumin (group VI). The cytological (mitotic index, chromosomal abnormalities and DNA damage), physiological (rooting percentage, root length, growth rate and weight gain) and oxidative stress (malondialdehyde level, superoxide dismutase level, catalase level and glutathione reductase level) indicators were determined after 96 h of treatment. The results revealed that pendimethalin treatment reduced rooting percentage, root length, growth rate and weight gain whereas induced chromosomal abnormalities and DNA damage in roots of A. cepa L. Further, pendimethalin exposure elevated malondialdehyde level followed by antioxidant enzymes. The activities of superoxide dismutase and catalase were up-regulated and glutathione reductase was down-regulated. The molecular docking supported the antioxidant enzymes activities result. However, a dose-dependent reduction of pendimethalin toxicity was observed when curcumin was supplied with pendimethalin. The maximum recovery of cytological, physiological and oxidative stress parameters was recorded at 10 mg/L concentration of curcumin. The correlation studies also revealed positive relation of curcumin with rooting percentage, root length, weight gain, mitotic activity and glutathione reductase enzyme level while an inverse correlation was observed with chromosomal abnormalities, DNA damage, superoxide dismutase and catalase enzyme activities, and lipid peroxidation indicating its protective effect.
Arm-to-retina time predicts visual outcome of anti-vascular endothelial growth factor treatment for macular edema due to central retinal vein occlusion
Authors: Takei Tatsuhiko , Nagai Norihiro , Ohkoshi Kishiko , Ozawa Yoko 
Published: February 2022

AbstractTo explore the factors associated with best-corrected visual acuity (BCVA) after anti-vascular endothelial growth factor (anti-VEGF) treatment for macular edema secondary to central retinal vein occlusion (CRVO). We retrospectively reviewed the medical charts of 22 eyes of 22 treatment-naïve patients with CRVO diagnosed between September 2014 and December 2020. They received anti-VEGF treatment and follow-up for > 12 months. Mean patient age was 64.3 years; 13 (59.1%) were men. Eyes with baseline arm-to-retina (AR) time ≥ 16 s had better BCVA at 12 months (adjusted for baseline BCVA and age; B, − 0.658; 95% confidence interval − 1.058 to − 0.257; P = 0.003), greater mean BCVA change (P = 0.006), lower frequency of residual macular edema at 12 months (P = 0.026) and recurrent and/or unresolved macular edema during 12 months (P = 0.046), and higher frequency of reduction in central retinal thickness ≥ 150 μm at 1 and 12 months (both P = 0.046). Delayed AR time was associated with a better visual outcome and macular edema improvement in CRVO after anti-VEGF treatment regardless of initial BCVA and age. Our results may help understand the pathogenesis and predict the visual prognosis of patients before anti-VEGF therapy initiation.
Bis(5-chloroquinolin-8-olato)-bis(pyridine)-cobalt(II) as new catalytic material
Authors: Drzeżdżon Joanna , Mokwa Celina , Sikorski Artur , Parnicka Patrycja , Zaleska-Medynska Adriana , Malinowski Jacek , Kwiatkowska Magdalena , Gawdzik Barbara , Jacewicz Dagmara 
Published: February 2022

AbstractNowadays, studies are carried out on the design and synthesis of new catalysts for olefin oligomerization and polymerization, which would contain non-toxic metals and at the same time show high catalytic activities. Complex compounds of transition metal ions such as Fe(II), Cr(III) and Zr(II) containing pyridine or quinoline as ligands show at least moderate catalytic activity in ethylene and propylene polymerizations. To investigate the catalytic activity of the complex containing pyridine ligands and quinoline derivatives, here we have synthesized the crystals of new bis(5-chloroquinolin-8-olato)-bis(pyridine)-cobalt(II) solvate. The synthesized cobalt(II) complex compound was tested in reactions of 2-chloro-2-propen-1-ol and norbornene oligomerizations. Our studies showed that bis(5-chloroquinolin-8-olato)-bis(pyridine)-cobalt(II) after activation by MMAO-12 catalyzes the formation of oligomers in nitrogen atmosphere, under atmospheric pressure and at room temperature. Bis(5-chloroquinolin-8-olato)-bis(pyridine)-cobalt(II) possesses moderate catalytic activity in the formation of norbornene oligomers process and low catalytic activity in 2-chloro-2-propen-1-ol oligomerization.
Identification and validation of a cigarette smoke-related five-gene signature as a prognostic biomarker in kidney renal clear cell carcinoma
Authors: Huang Yefei , Wang Qinzhi , Tang Yu , Liu Zixuan , Sun Guixiang , Lu Zhaojun , Chen Yansu 
Published: February 2022

AbstractCigarette smoking greatly promotes the progression of kidney renal clear cell carcinoma (KIRC), however, the underlying molecular events has not been fully established. In this study, RCC cells were exposed to the tobacco specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK, nicotine-derived nitrosamine) for 120 days (40 passages), and then the soft agar colony formation, wound healing and transwell assays were used to explore characteristics of RCC cells. RNA-seq was used to explore differentially expressed genes. We found that NNK promoted RCC cell growth and migration in a dose-dependent manner, and RNA-seq explored 14 differentially expressed genes. In TCGA-KIRC cohort, Lasso regression and multivariate COX regression models screened and constructed a five-gene signature containing ANKRD1, CYB5A, ECHDC3, MT1E, and AKT1S1. This novel gene signature significantly associated with TNM stage, invasion depth, metastasis, and tumor grade. Moreover, when compared with individual genes, the gene signature contained a higher hazard ratio and therefore had a more powerful value for the prognosis of KIRC. A nomogram was also developed based on clinical features and the gene signature, which showed good application. Finally, AKT1S1, the most crucial component of the gene signature, was significantly induced after NNK exposure and its related AKT/mTOR signaling pathway was dramatically activated. Our findings supported that NNK exposure would promote the KIRC progression, and the novel cigarette smoke-related five-gene signature might serve as a highly efficient biomarker to identify progression of KIRC patients, AKT1S1 might play an important role in cigarette smoke exposure-induced KIRC progression.
Understanding the ZIF-L to ZIF-8 transformation from fundamentals to fully costed kilogram-scale production
Authors: Deacon Adam , Briquet Ludovic , Malankowska Magdalena , Massingberd-Mundy Felicity , Rudić Svemir , Hyde Timothy l. , Cavaye Hamish , Coronas Joaquín , Poulston Stephen , Johnson Timothy 
Published: February 2022

AbstractThe production of MOFs at large scale in a sustainable way is key if these materials are to be exploited for their promised widespread application. Much of the published literature has focused on demonstrations of preparation routes using difficult or expensive methodologies to scale. One such MOF is nano-zeolitic imidazolate framework-8 (ZIF-8) – a material of interest for a range of possible applications. Work presented here shows how the synthesis of ZIF-8 can be tracked by a range of methods including X-ray diffraction, thermo gravimetric analysis and inelastic neutron scattering – which offer the prospect of in-line monitoring of the synthesis reaction. Herein we disclose how the production of nano-ZIF-8 can be conducted at scale using the intermediate phase ZIF-L. By understanding the economics and demonstrating the production of 1 kg of nano-ZIF-8 at pilot scale we have shown how this once difficult to make material can be produced to specification in a scalable and cost-efficient fashion.
Alpine rockwall erosion patterns follow elevation-dependent climate trajectories
Authors: Draebing Daniel , Mayer Till , Jacobs Benjamin , McColl Samuel T. 
Published: February 2022

AbstractMountainous topography reflects an interplay between tectonic uplift, crustal strength, and climate-conditioned erosion cycles. During glaciations, glacial erosion increases bedrock relief, whereas during interglacials relief is lowered by rockwall erosion. Here, we show that paraglacial, frost cracking and permafrost processes jointly drive postglacial rockwall erosion in our research area. Field observations and modelling experiments demonstrate that all three processes are strongly conditioned by elevation. Our findings on catchment scale provide a potential multi-process explanation for the increase of rockwall erosion rates with elevation across the European Alps. As alpine basins warm during deglaciation, changing intensities and elevation-dependent interactions between periglacial and paraglacial processes result in elevational shifts in rockwall erosion patterns. Future climate warming will shift the intensity and elevation distribution of these processes, resulting in overall lower erosion rates across the Alps, but with more intensified erosion at the highest topography most sensitive to climate change.
Malaria elimination on Hainan Island despite climate change
Authors: Tian Huaiyu , Li Naizhe , Li Yapin , Kraemer Moritz U. G. , Tan Hua , Liu Yonghong , Li Yidan , Wang Ben , Wu Peiyi , Cazelles Bernard , Lourenço José , Gao Dongqi , Sun Dingwei , Song Wenjing , Li Yuchun , Pybus Oliver G. , Wang Guangze , Dye Christopher 
Published: February 2022

Abstract
Background
Rigorous assessment of the effect of malaria control strategies on local malaria dynamics is a complex but vital step in informing future strategies to eliminate malaria. However, the interactions between climate forcing, mass drug administration, mosquito control and their effects on the incidence of malaria remain unclear.

Methods
Here, we analyze the effects of interventions on the transmission dynamics of malaria (Plasmodium vivax and Plasmodium falciparum) on Hainan Island, China, controlling for environmental factors. Mathematical models were fitted to epidemiological data, including confirmed cases and population-wide blood examinations, collected between 1995 and 2010, a period when malaria control interventions were rolled out with positive outcomes.

Results
Prior to the massive scale-up of interventions, malaria incidence shows both interannual variability and seasonality, as well as a strong correlation with climatic patterns linked to the El Nino Southern Oscillation. Based on our mechanistic model, we find that the reduction in malaria is likely due to the large scale rollout of insecticide-treated bed nets, which reduce the infections of P. vivax and P. falciparum malaria by 93.4% and 35.5%, respectively. Mass drug administration has a greater contribution in the control of P. falciparum (54.9%) than P. vivax (5.3%). In a comparison of interventions, indoor residual spraying makes a relatively minor contribution to malaria control (1.3%–9.6%).

Conclusions
Although malaria transmission on Hainan Island has been exacerbated by El Nino Southern Oscillation, control methods have eliminated both P. falciparum and P. vivax malaria from this part of China.
Geschlechtsspezifische Unterschiede in der Pharmakologie
Authors: Fender Anke C. , Dobrev Dobromir 
Published: February 2022

ZusammenfassungFrauen erfahren Krankheit und Pharmakotherapie oft anders als Männer. Neben
psychosozialen Aspekten bestehen geschlechtsspezifische Unterschiede auch bei Prozessen
der Pharmakokinetik (Aufnahme, Verteilung, Metabolisierung, Ausscheidung) sowie der
Pharmakodynamik (Bindung, biologische und molekulare Wirkung). Diese Variabilität kann die
Wirkdauer, Wirkstärke und Wirkqualität eines Arzneistoffs entscheidend beeinflussen und
sollte bei der individuellen Nutzen-Risiko-Abwägung unbedingt einbezogen werden.
Pulmonalarterielle Hypertonie bei Frauen
Authors: Xanthouli Panagiota , Eichstaedt Christina A. , Ewinger Max , Marra Alberto M. , Grünig Ekkehard 
Published: February 2022

ZusammenfassungDie pulmonalarterielle Hypertonie (PAH) ist eine seltene Erkrankung mit vielen
Untergruppen. Frauen sind wesentlich häufiger betroffen bei der „klassischen“
idiopathischen Form, der hereditären und bei der Kollagenose-assoziierten PAH. Es wird
vermutet, dass der höhere Östrogenspiegel bei Frauen die Pathophysiologie mit Einengung
der kleinen Pulmonalarteriolen als Ursache der PAH zumindest bei den Mutationsträgerinnen
begünstigt. PAH-Patientinnen haben ähnliche Symptome wie betroffene Männer, sprechen aber
möglicherweise anders auf gezielte PAH-Therapien an. Sie haben im Mittel ein längeres
Überleben im Vergleich zu männlichen Patienten. In dieser Arbeit werden wir