bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical significance of 18F-FDG PET/CT imaging in 32 cases of gastrointestinal stromal tumors
Authors: Du Wen , Cui Guojin , Wang Kaiping , Li Shaojie 
Published: September 2022

Abstract
Objectives
To explore the clinical significance of 18F-FDG metabolic imaging in the diagnosis and biological risk assessment of gastrointestinal stromal tumors (GIST).

Methods
This study is a clinical retrospective study. The research subjects were patients with GIST who were admitted to our hospital from January 2014 to December 2019 and underwent 18F-FDG metabolic imaging, and the relationship between biological risk and FDG metabolism was analyzed retrospectively.

Results
A total of 32 patients with GIST were included in this study, of which 17 patients had very low and low-risk lesions, and the FDG metabolism level did not increase; five patients had moderate-risk gastric lesions, and the FDG metabolism level was abnormally increased; 10 patients had high-risk lesions, and except for one patient with multiple lesions, the FDG metabolism level of these patients was increased.

Conclusions
The level of glucose metabolism is abnormally increased in tumor cells with vigorous mitosis and has higher biological risk. The 18F-FDG metabolism level can determine the biological risk of GIST and whether high-risk lesions involve other tissues and organs, as it more comprehensively reflects the distribution of lesions, the activity of tumor cells and the stage of the disease.
General anesthesia with remimazolam for a pediatric patient with MELAS and recurrent epilepsy: a case report
Authors: Yamadori Yusuke , Yamagami Yuki , Matsumoto Yukihisa , Koizumi Mari , Nakamura Akiyo , Mizuta Daiskuke , Yasuda Kyoko , Shirakami Gotaro 
Published: September 2022

Abstract
Background
Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is a mitochondrial disease. We report here the safe use of remimazolam in a pediatric MELAS patient.

Case presentation
A 10-year-old girl (118 cm, 16 kg) was scheduled for an open gastrostomy to improve nutrition and epileptic seizure control. We induced and maintained general anesthesia with remimazolam, remifentanil, fentanyl, and rocuronium. We also performed a bilateral subcostal transversus abdominis plane block before the surgery. The surgery finished uneventfully. After we discontinued remimazolam administration, the patient woke up immediately but calmly without flumazenil. Epileptic seizures did not occur during intra- and early post-operative periods.

Conclusion
Remimazolam enabled us to provide a pediatric MELAS patient with general anesthesia without causing delayed emergence or epileptic seizures.
Validity evidence of the Brazilian version of the Perceived Motivational Climate in Sports Questionnaire-2 (PMCSQ-2BR)
Authors: Arthur Daniel Perez , Paes Mayara Juliana , do Amaral Machado Thais , Stefanello Joice Mara Facco 
Published: September 2022

AbstractIn sports context, the motivational climate has been widely studied since 2000, when the Perceived Motivational Climate in Sports Questionnaire-2 (PMCSQ-2) was published. Evaluating athletes’ perceptions of the motivational climate created by the coach, this questionnaire has been validated and adapted for different countries, including Brazil. However, important psychometric properties of the Brazilian version present problems, such as poor fit indices and the almost exclusively male samples, make it unfeasible for use in future research. Thus, this study aimed to achieve a new process of cross-cultural adaptation, and search for validity evidence of the PMCSQ-2 for the Brazilian sports context to correct distortions, expand the sources of evidence of validity and ecological validity, and make it suitable for application in future research. The sample consisted of 501 athletes (349 males, 152 females) from different sports. The findings of the current study support the multidimensional hierarchical characteristic of the instrument, its factorial structure, and internal consistency. We concluded that the 33-item PMCSQ-2BR, distributed in two high-order scales (ego-involving and task-involving) with three subscales each, can be used to assess athletes’ perceived motivational climate in the Brazilian sports context.
Neisseria meningitidis carriage rate, antibiotic susceptibility profile, and associated factors among prisoners at Jimma zonal correction facility in Jimma Town, Southwestern Ethiopia: a cross-sectional study
Authors: Assefa Samuel , Abdissa Alemseged , Alemu Yared , Girma Lencho , Sahlu Degemu 
Published: September 2022

Abstract
Background
Neisseria meningitidis causes severe life-threatening meningococcal disease with a case fatality rate of 10–15% even with proper treatment. In Ethiopia, particularly in our study area, inadequate information is found on meningococcal disease. So, this study aimed to assess N. meningitidis carriage rate, antibiotic susceptibility profile, and associated factors among prisoners in Jimma Town, Southwestern Ethiopia.

Methods
A cross-sectional study was conducted in Jimma town, Southwest Ethiopia, from May to October 2019. A stratified sampling technique was used and proportional allocation was done. A total of 550 oropharyngeal swabs were collected, processed, isolated, and identified N. meningitidis using standard microbiological techniques. Antibiotics susceptibility test was done for isolates using the disk diffusion method. Data on demographic and associated factors for carriage were collected using a structured questionnaire. Data were summarized using frequency, percentage, graph, and table. A logistic regression model was used to see the association between the dependent and independent variables. Variables with a p-value < 0.25 during bivariate analysis were included in multivariate analysis to identify factors significantly associated with the meningococcal carriage and, a p-value < 0.05 was considered statistically significant.

Result
Out of the 550 study participants, 76(13.8%) with (CI: 7.20–18.20) were found carriers of N meningitidis. The predominant isolates were non-serogroupable 26(34.2%) and serogroup W/Y 22(28.9%), respectively. N. meningitidis isolates showed highest sensitivity to chloramphenicol 74(97.4%). Meningococcal carriage rate was significantly associated with being age group of 16–20 years; having respiratory symptoms within 3 months and active cigarette smoking within 3 months.

Conclusions
The majority of participants harbor most of the serogroups responsible for invasive cases of meningococcal disease. Respiratory symptoms, active cigarette smoking, and age group of 16–20 years increased the risk of N. meningitidis pharyngeal carriage rate. This study suggests providing better health education to control respiratory symptoms, smoking, and providing antibiotic prophylaxis for prisoners.
Translation, linguistic validation and reliability of FACT-H&N questionnaire in Oral Cancer patients in Sudan
Authors: Sheikh M. El , Suleiman A. , Satti A. , O’Sullivan E. M. 
Published: September 2022

Abstract
Background
This study aimed to translate and validate an Arabic version of the Functional Assessment of Cancer Therapy Head and Neck Scale (FACT-H&N, v-4) for use among Sudanese oral cancer patients.

Methods
The instrument underwent translation and validation following the standard FACT translation methodology. The translated instrument was pre-tested for face validity and content validity using semi-structured, in-depth interviews with ten oral cancer patients to assess acceptability. The questionnaire was pilot tested with 60 patients; reliability was tested for internal consistency with Cronbach’s alpha while construct validity was tested using ‘known-group validity’.

Results
The pre-test study revealed no major issues, apart from a reluctance to answer questions on sexual satisfaction. The FACT-H&N demonstrated good internal consistency, it considered five particular constructs: PWB, SWB, EWB, FWB and FACT-H&N, their Cronbach’s α values were positive and close to 1 with values of 0.85, 0.788, 0.86, 0.895 and 0.703 respectively, indicating that the questionnaire was valid and the responses consistent. Sixty patients were asked the global health-related quality of life item, 36.3% rated their QOL as very good or good (36.3%), while 41.7% rated it as average, and 21.7% as poor or very poor. Then FACT subscale mean scores were tabulated against three categories; patients with very poor/poor recorded significantly lower scores indicating construct validity. Some psychometric properties were consistent with other FACT-H&N translations such as the Chinese, French, Pakistani and Malaysian.

Conclusions
This study validates the Arabic version of the FACT-H&N. It is a reliable tool and, will assist further QoL research in other Arabic-speaking countries.
Granulosa Cell Dysfunction Is Associated With Diminished Ovarian Response in FMR1 Premutation Carriers
Authors: Boustanai Ilana , Raanani Hila , Aizer Adva , Orvieto Raoul , Elizur Shai E 
Published: September 2022

Abstract

Context
FMR1 premutation (PM) carriers are at increased risk of ovarian impairment resulting in diminished ovarian response (DOR) to exogenous follicle-stimulating hormone (FSH) stimulation. Expanded CGG repeat transcript and RAN-associated protein (FMRpolyG) have been shown to accumulate in cellular aggregates and sequester proteins, thus impairing their function. Sam68 is a multifunctional RNA-binding protein highly expressed in the gonads involved in FSH receptor (FSHR) transcript maturation during FSH-dependent follicular development.

Objective
The present study examined a possible pathophysiological explanation for DOR to exogenous FSH stimulation in FMR1 PM carriers.

Methods
We used both a human granulosa cell (GC) line model and human GCs from FMR1 PM carriers to evaluate whether Sam68 is sequestered with expanded CGG repeat transcript.

Results
We show that Sam68 is sequestered in GCs, most likely by interaction with the expanded CGG repeat transcript. The sequestration may lead to reduced levels of free Sam68 available for FHSR precursor transcript processing, causing dysregulation of FSHR transcript maturation, and a consequent decrease in FSHR protein levels.

Conclusion
Sam68 sequestration may underlie the diminished ovarian response to FSH stimulation in FMR1 PM carriers.
Cervical MUC5B and MUC5AC are Barriers to Ascending Pathogens During Pregnancy
Authors: Ueda Yusuke , Mogami Haruta , Kawamura Yosuke , Takakura Masahito , Inohaya Asako , Yasuda Eriko , Matsuzaka Yu , Chigusa Yoshitsugu , Ito Shinji , Mandai Masaki , Kondoh Eiji 
Published: September 2022

Abstract

Context
Cervical excision is a risk factor for preterm birth. This suggests that the cervix plays an essential role in the maintenance of pregnancy.

Objective
We investigated the role of the cervix through proteomic analysis of cervicovaginal fluid (CVF) from pregnant women after trachelectomy surgery, the natural model of a lack of cervix.

Methods
The proteome compositions of CVF in pregnant women after trachelectomy were compared with those in control pregnant women by liquid chromatography–tandem mass spectrometry and label-free relative quantification. MUC5B/AC expression in the human and murine cervices was analyzed by immunohistochemistry. Regulation of MUC5B/AC expression by sex steroids was assessed in primary human cervical epithelial cells. In a pregnant mouse model of ascending infection, Escherichia coli or phosphate-buffered saline was inoculated into the vagina at 16.5 dpc, and the cervices were collected at 17.5 dpc.

Results
The expression of MUC5B/5AC in cervicovaginal fluid was decreased in pregnant women after trachelectomy concomitant with the anatomical loss of cervical glands. Post-trachelectomy women delivered at term when MUC5B/AC abundance was greater than the mean normalized abundance of the control. MUC5B levels in the cervix were increased during pregnancy in both humans and mice. MUC5B mRNA was increased by addition of estradiol in human cervical epithelial cells, whereas MUC5AC was not. In a pregnant mouse model of ascending infection, E. coli was trapped in the MUC5B/AC-expressing mucin of the cervix, and neutrophils were colocalized there.

Conclusion
Endocervical MUC5B and MUC5AC may be barriers to ascending pathogens during pregnancy.
Acute Sleep Disruption Does Not Diminish Pulsatile Growth Hormone Secretion in Pubertal Children
Authors: Calvert Madison E , Molsberry Samantha A , Kangarloo Tairmae , Amin Md Rafiul , Genty Valentina , Faghih Rose T , Klerman Elizabeth B , Shaw Natalie D 
Published: September 2022

Abstract

Context
In children, growth hormone (GH) pulses occur after sleep onset in association with slow-wave sleep (SWS). There have been no studies in children to quantify the effect of disrupted sleep on GH secretion.

Objective
This study aimed to investigate the effect of acute sleep disruption on GH secretion in pubertal children.

Methods
Fourteen healthy individuals (aged 11.3-14.1 years) were randomly assigned to 2 overnight polysomnographic studies, 1 with and 1 without SWS disruption via auditory stimuli, with frequent blood sampling to measure GH.

Results
Auditory stimuli delivered during the disrupted sleep night caused a 40.0 ± 7.8% decrease in SWS. On SWS-disrupted sleep nights, the rate of GH pulses during N2 sleep was significantly lower than during SWS (IRR = 0.56; 95% CI, 0.32-0.97). There were no differences in GH pulse rates during the various sleep stages or wakefulness in disrupted compared with undisrupted sleep nights. SWS disruption had no effect on GH pulse amplitude and frequency or basal GH secretion.

Conclusion
In pubertal children, GH pulses were temporally associated with episodes of SWS. Acute disruption of sleep via auditory tones during SWS did not alter GH secretion. These results indicate that SWS may not be a direct stimulus of GH secretion.
Patterns of Panethnic Intermarriage in the United States, 1980–2018
Authors: Gullickson Aaron 
Published: September 2022

Abstract
Intermarriage among ethnic groups belonging to the same panethnic category (e.g., Asian, Latino) is an important indicator of the strength of panethnicity. Yet, most of the research on panethnic intermarriage uses older samples with significant data limitations. In this article, I use data on recently married couples from the 2014–2018 American Community Surveys and the 1980 U.S. Census to analyze the likelihood of ethnic exogamy within the panethnic categories of Latino, East/Southeast Asian, and South Asian. I utilize a counterfactual marriage model that accounts for group size within local marriage markets, eliminates immigrants married abroad from analysis, and controls for birthplace and language endogamy. The results show that birthplace and language diversity are significant barriers to ethnic exogamy among Asians but not Latinos. Once birthplace and language endogamy are held constant, panethnic intermarriage is far more likely among Asians than among Latinos. East/Southeast Asian ethnic exogamy has increased over time, while Latino ethnic exogamy has not. Furthermore, East/Southeast Asian and South Asian intermarriage remains rare, suggesting that panethnic intermarriage among Asians occurs within two separate melting pots.
MLL regulates the actin cytoskeleton and cell migration by stabilising Rho GTPases via the expression of RhoGDI1
Authors: Chinchole Akash , Lone Kaisar Ahmad , Tyagi Shweta 
Published: September 2022

ABSTRACT
Attainment of proper cell shape and the regulation of cell migration are essential processes in the development of an organism. The mixed lineage leukemia (MLL or KMT2A) protein, a histone 3 lysine 4 (H3K4) methyltransferase, plays a critical role in cell-fate decisions during skeletal development and haematopoiesis in higher vertebrates. Rho GTPases – RhoA, Rac1 and CDC42 – are small G proteins that regulate various key cellular processes, such as actin cytoskeleton formation, the maintenance of cell shape and cell migration. Here, we report that MLL regulates the homeostasis of these small Rho GTPases. Loss of MLL resulted in an abnormal cell shape and a disrupted actin cytoskeleton, which lead to diminished cell spreading and migration. MLL depletion affected the stability and activity of Rho GTPases in a SET domain-dependent manner, but these Rho GTPases were not direct transcriptional targets of MLL. Instead, MLL regulated the transcript levels of their chaperone protein RhoGDI1 (also known as ARHGDIA). Using MDA-MB-231, a triple-negative breast cancer cell line with high RhoGDI1 expression, we show that MLL depletion or inhibition by small molecules reduces tumour progression in nude mice. Our studies highlight the central regulatory role of MLL in Rho/Rac/CDC42 signalling pathways.
This article has an associated First Person interview with the first author of the paper.
High duty cycle moth sounds jam bat echolocation: bats counter with compensatory changes in buzz duration
Authors: Fernández Yohami , Dowdy Nicolas J. , Conner William E. 
Published: September 2022

ABSTRACT
Tiger moth species vary greatly in the number of clicks they produce and the resultant duty cycle. Signals with higher duty cycles are expected to more effectively interfere with bat sonar. However, little is known about the minimum duty cycle of tiger moth signals for sonar jamming. Is there a threshold that allows us to classify moths as acoustically aposematic versus sonar jammers based on their duty cycles? We performed playback experiments with three wild-caught adult male bats, Eptesicus fuscus. Bat attacks on tethered moths were challenged using acoustic signals of Bertholdia trigona with modified duty cycles ranging from 0 to 46%. We did not find evidence for a duty cycle threshold; rather, the ability to jam the bat's sonar was a continuous function of duty cycle consistent with a steady increase in the number of clicks arriving during a critical signal processing time window just prior to the arrival of an echo. The proportion of successful captures significantly decreased as the moth duty cycle increased. Our findings suggest that moths cannot be unambiguously classified as acoustically aposematic or sonar jammers based solely on duty cycle. Bats appear to compensate for sonar jamming by lengthening the duration of their terminal buzz and they are more successful in capturing moths when they do so. In contrast to previous findings for bats performing difficult spatial tasks, the number of sonar sound groups decreased in response to high duty cycles and did not affect capture success.
Evaluating the ‘cost of generating force’ hypothesis across frequency in human running and hopping
Authors: Allen Stephen P. , Beck Owen N. , Grabowski Alena M. 
Published: September 2022

ABSTRACT
The volume of active muscle and duration of extensor muscle force well explain the associated metabolic energy expenditure across body mass and velocity during level-ground running and hopping. However, if these parameters fundamentally drive metabolic energy expenditure, then they should pertain to multiple modes of locomotion and provide a simple framework for relating biomechanics to metabolic energy expenditure in bouncing gaits. Therefore, we evaluated the ability of the ‘cost of generating force’ hypothesis to link biomechanics and metabolic energy expenditure during human running and hopping across step frequencies. We asked participants to run and hop at 85%, 92%, 100%, 108% and 115% of preferred running step frequency. We calculated changes in active muscle volume, duration of force production and metabolic energy expenditure. Overall, as step frequency increased, active muscle volume decreased as a result of postural changes via effective mechanical advantage (EMA) or duty factor. Accounting for changes in EMA and muscle volume better related to metabolic energy expenditure during running and hopping at different step frequencies than assuming a constant EMA and muscle volume. Thus, to ultimately develop muscle mechanics models that can explain metabolic energy expenditure across different modes of locomotion, we suggest more precise measures of muscle force production that include the effects of EMA.
Women physicians, their social issues & Barriers to their success in Academic Medicine
Authors: Jawaid Shaukat Ali 
Published: September 2022

doi: https://doi.org/10.12669/pjms.38.8.7047
How to cite this:Jawaid SA. Women physicians, their social issues & Barriers to their success in Academic Medicine. Pak J Med Sci. 2022;38(8):2049-2052. doi: https://doi.org/10.12669/pjms.38.8.7047
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Policy Brief 10: Using anti-money laundering frameworks to fight illegal wildlife trade in Uganda
Authors: Walugembe Tom 
Published: September 2022

In February 2020, Uganda made a high-level political commitment to work with the Financial Action Task Force (FATF) and Eastern and Southern Africa Anti-Money Laundering Group (ESAAMLG) to strengthen the effectiveness of its anti-money laundering (AML) regime. Among other commitments, Uganda undertook to demonstrate that law enforcement agencies and judicial authorities apply the money laundering offence consistent with the identified risks.
Studies show that Uganda has high risks for cross-border movement of illegally obtained wildlife and wildlife products, both as a source and transit country. In addition, Uganda and the East African region in general have made some massive seizures of illegal wildlife products, which points to organised criminal activity. Notably, no money laundering prosecutions have to date arisen out of illegal wildlife trade (IWT) as a predicate offence in Uganda, Kenya and Tanzania.
This short Policy Brief provides an outline of how the AML framework (intelligence, investigations and prosecutions) can be utilised to help combat IWT and the corruption that facilitates it. The insights are drawn from the Ugandan context, but can be applied with appropriate adjustments to other jurisdictions seeking to strengthen efforts to combat IWT and related corruption/money laundering.
Working Paper 40: Developing anti-corruption interventions addressing social norms
Authors: Baez Camargo Claudia 
Published: September 2022

This Working Paper provides guidance on developing anti-corruption interventions based on a Social Norms and Behaviour Change (SNBC) approach. Still a relatively nascent field, SNBC interventions typically address social norms that make corruption acceptable or expected, and attempt to influence behaviours away from corrupt practices.
The guidance is based on lessons learned from a largely successful pilot project in Tanzania that targeted social norms fuelling bribery ("gift giving") in health facilities and attempted to change the behaviours of both health care providers and users away from exchanging gifts. Survey results showed a 14–44% decrease in gift-giving intentions, attitudes and positive beliefs among hospital users following the pilot intervention.
The guidance covers:

How to identify when a SNBC approach is suitable
Essential background research needed to design anti-corruption SNBC interventions
Frameworks to formulate theories of change
Specific elements to build into SNBC interventions
What practitioners should expect when embarking on an SNBC intervention
Ways they can help build evidence and understanding of SNBC approaches in the anti-corruption field.

About and acknowledgements
This publication was supported by the Deutsche Gesellschaft für Internationale Zusammenarbeit (GIZ) GmbH on behalf of the Federal Ministry for Economic Cooperation and Development (BMZ). The contents of this publication do not represent the official position of either BMZ or GIZ.
The pilot intervention that serves as the basis for most of the reflections included in this document was funded by the Global Integrity Anti-Corruption Evidence Programme (GI-ACE), funded with UK aid from the UK government.