bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti‐proliferative and metastasis‐inhibiting effect of carbon ions on non‐small cell lung adenocarcinoma A549 cells
Authors: Yang Zhen , Luo Hongtao , Feng Shuangwu , Geng Yichao , Zhao Xueshan , Li Chengcheng , Liu Ruifeng , Zhang Qiuning , Wang Xiaohu 
Published: April 2022

AbstractObjectiveCarbon ions suppress the effect of glutathione peroxidase 4 (GPX4) on the proliferation and metastasis of lung adenocarcinoma A549 cells.MethodsThe clonogenic formation ability of A549 cells was calculated with different ray doses of radiation. Cell proliferation, cycle, and apoptosis of A549 cells after different ray irradiation were detected by cck8 and flow cytometry. The transwell assay was used to detect the cell migration and invasion after different rays of radiation to A549 cells. The GPX4 matrix metalloproteinase 2 and E‐cadherin expression levels were assessed by immunofluorescence and western blot.ResultsAt the dose of 4 Gy, A549 cell viability was 22.95 ± 5.00% and 55.52 ± 3.26% when treated with carbon ions and X‐rays, respectively. The distribution of A549 cells arrested in the G2 phase by carbon ion irradiation was positively correlated with radiation dose, with a most significant increase at 4 Gy irradiation (t = 4.303, p < 0.05). The results of apoptosis after irradiation showed were no significant differences in apoptosis at 1 and 2 Gy of X‐ray compared with 0 Gy, and 4 Gy irradiation significantly induced apoptosis in A549 cells (t = 30.520, p < 0.05). The apoptotic rate of carbon ion‐irradiated cells increased in a dose‐dependent manner and was the highest at 4 Gy (t = 17.356, p < 0.05). The transwell assays showed significantly decreased migration and invasion of both ray‐irradiated cells compared with the 0 Gy control cells. The carbon ions of 2 and 4 Gy significantly inhibited A549 cell migration (t2Gy = 9.260, t4Gy = 19.052, both p < 0.05) and invasion compared with those in the control cells (t2Gy = 6.141, t4Gy = 12.700, both p < 0.05). Treatment with 2 and/or 4 Gy doses of carbon ion reduced GPX4 (t2Gy = 8.170, t4Gy = 13.811, both p < 0.05) and matrix metalloproteinase 2 (t2Gy = 207.620, t4Gy = 93.170, both p < 0.05) protein expression, but E‐cadherin expression was increased (t2Gy = 62.811, t4Gy = 102.106, both p < 0.05).ConclusionCompared with X‐ray irradiation, carbon ions have a higher relative biological effectiveness and can increase the proportion of A549 cells arrested in the G2/M stage to induce apoptosis, thereby inhibiting proliferation. At the same dose with X‐ray, carbon ions may inhibit the migration and invasion of A549 cells by regulating the expression of GPX4, matrix metalloproteinase 2, and E‐cadherin.
Antagonistically Functionalized Diatom Biosilica for Bio‐Triboelectric Generators
Authors: Rajabi‐Abhari Araz , Lee Jeehee , Tabassian Rassoul , Kim Jong‐Nam , Lee Haeshin , Oh Il‐Kwon 
Published: April 2022

AbstractAlthough biomaterial‐based triboelectric nanogenerators (Bio‐TENGs) for use in wearable electronics and implantable sensors have been developed, power generation is not suitable for satisfying the basic requirements for practical applications. Here, to greatly enhance output performances of Bio‐TENG devices, an antagonistic approach of diatom frustules (DFs) with amine and fluorine chemical functionalizations is reported. The DFs are treated with piranha solution to increase the density of hydroxyl groups and tribo‐positive and tribo‐negative composite films are designed with antagonistically functionalized DFs. The tribo‐positive composites having electron donating functionality consist of aminated DFs and cellulose nanocrystals (CNCs), while the tribo‐negative composite is composed of fluorinated DFs and polydimethylsiloxane (PDMS). An antagonistically and chemically functionalized TENG (ACF TENG) with an efficient contact area of 9.6 cm2 under a force of 8 N and a frequency of 5 Hz exhibits an output voltage of 248 V, a short‐circuit current of 16.4 µA, and a power density of 2.01 W m−2, which is 16.6 times higher than a reference (CNC:PDMS) TENG. This study shows a simple antagonistic approach for chemical functionalization as an efficient method to manipulate the tribo‐polarity of bio‐additives for enhancing power generation of Bio‐TENGs.
LINC00365 inhibited lung adenocarcinoma progression and glycolysis via sponging miR‐429/KCTD12 axis
Authors: Zhang Cheng‐Wei , Zhou Bin , Liu Yan‐Chao , Su Li‐Wei , Meng Jie , Li Shao‐Lei , Wang Xue‐Long 
Published: April 2022

AbstractThis study researched the function of long non‐coding RNA LINC00365 in lung adenocarcinoma (LAD) progression. LINC00365, miR‐429, and KCTD12 expression in the LAD clinical tissues and cells were detcetd by qRT‐PCR and Western blot. LINC00365, miR‐429, and KCTD12 effects on H1975 cells malignant phenotype were detected by cell counting kit‐8 assay, clone formation experiment, Transwell experiment, and glycolysis. Dual luciferase reporter gene assay and RNA pull‐down assay were implemented. LINC00365 effect on H1975 cells in vivo growth was detected. LINC00365 was low expressed in the LAD patients and cells, associating with poor outcome. LINC00365 up‐regulation attenuated H1975 cells proliferation, migration, invasion, glycolysis and in vivo growth. LINC00365 inhibited KCTD12 expression by sponging miR‐429. miR‐429 up‐regulation and KCTD12 down‐regulation partial reversed LINC00365 inhibition on H1975 cells malignant phenotype. Thus, LINC00365 inhibited LAD progression and glycolysis via targeting miR‐429/KCTD12 axis. LINC00365 might be a potential candidate for LAD target treatment clinically.
Local Stability to Periodicity in the EuMg5+x Type: Chemical Pressure, Disordered Channels, and Predicted Superstructure in YZn5.225
Authors: Fredrickson Rie T. , Kamp Kendall R. , Fredrickson Daniel C. 
Published: April 2022

AbstractA central theme in the structural chemistry of intermetallic phases is that complex structures can be derived from variations on simpler ones. This is vividly demonstrated by the variety of structure types that can be connected to chemical pressure (CP)‐driven transformations of the simple CaCu5 type. In this Article, we investigate an intriguing addition to this family: the EuMg5‐type intermetallics, as exemplified by YZn5. As expected from the large negative CPs around the cations in CaCu5‐type structures, YZn5 exhibits tightened coordination environments around the cations. However, it also contains an unusually inhomogeneous atomic packing, particularly in channels running between the Y atoms along c. Our structural reinvestigation of YZn5 reveals a disordered occupation pattern of Zn atoms within these channels, consistent with the EuMg5+x type, a disordered variant of the EuMg5 type. DFT‐CP analysis indicates that the transition from the CaCu5 type to the YZn5+x structure indeed creates more compact Y environments, but strong tensions remain within the Zn sublattice. These include CP features on the channel walls that provide a mechanism for the communication of structural information between the channels and favorable cooperation in their occupation patterns. Based on these results, a structural model is proposed that explains an earlier observation of superstructure reflections in the diffraction patterns of ErZn5 corresponding to a √3×√3×3 supercell.
Heterotic Kerr-Schild Double Field Theory and its double Yang-Mills formulation
Authors: Lescano Eric , Roychowdhury Sourav 
Published: April 2022

Abstract
We present a formulation of heterotic Double Field Theory (DFT), where the fundamental fields are in O(D, D) representations. The theory is obtained splitting an O(D, D + K ) duality invariant DFT. This procedure produces a Green-Schwarz mechanism for the generalized metric, and a fundamental gauge field which transforms as a gauge connection only to leading order. After parametrization, the former induces a non-covariant transformation on the metric tensor, which can be removed considering field redefinitions, and an ordinary Green-Schwarz mechanism on the b-field. Within this framework we explore perturbative properties of heterotic DFT. We use a relaxed version of the generalized Kerr-Schild ansatz (GKSA), where the generalized background metric is perturbed up to quadratic order considering a single null vector and the gauge field is linearly perturbed before parametrization. Finally we compare the dynamics of the gauge field and the generalized metric in order to inspect the behavior of the classical double copy correspondence at the DFT level.
Are orthopaedic surgeons prepared? An analysis of severe casualties from the 2021 flash flood and mudslide disaster in Germany
Authors: Gathen Martin , Welle Kristian , Jaenisch Max , Kasapovic Adnan , Rommelspacher Charlotte , Novosel Suncana , Roos Jonas , Kabir Koroush 
Published: April 2022

Abstract
Background
The purpose of this study was to describe and analyse the most severe casualties from the flash flood and mudslides occurring on 14 July 2021 in Germany, focusing on patients who were treated in the closest and largest level I trauma centre in the region the disaster occurred.

Methods
A single-centre retrospective study design was employed, and all patients treated because of the flooding and mudslides who needed inpatient treatment were documented. Data on each patient’s demographic characteristics, type of injury, number of surgeries, duration of hospitalisation, operation time, revision rate, injury severity score (ISS), and complications were collected. The primary outcome measure was status at discharge.

Results
Within the first week after the flood, a total of 63 patients were documented. Forty-one patients were treated on an outpatient basis in the emergency unit, and 22 patients were hospitalised. Of those hospitalised, 15 patients needed surgical treatment in the operation theatre. The most common injuries were fractures of the lower extremity (n = 7) and soft tissue wounds (n = 4). Overall, 20 surgeries were performed; the mean hospital stay was 7.2 ± 6.4 days, and the mean ISS was 5.7 ± 2.7.

Conclusion
The July 2021 flood disaster was one of the largest in German history. The included patients showed complex injuries of various types. Because of the effects of climate change, orthopaedic surgeons might face higher numbers of casualties affected by natural disasters. Learning more about the management and profile of these injuries can become a future challenge for orthopaedic and trauma surgeons.
The effects of nalmefene on the impulsive and reflective system in alcohol use disorder: A resting-state fMRI study
Authors: Grundinger Nadja , Gerhardt Sarah , Karl Damian , Mann Karl , Kiefer Falk , Vollstädt-Klein Sabine 
Published: April 2022

Abstract

Rationale
Central aspects of alcohol use disorder (AUD) are the irresistible desire for alcohol and impaired control over its intake. According to the triadic neurocognitive model of addiction, this arises from aberrant functioning of different neural and cognitive systems: an impulsive system, a reflective system, and the abnormal dynamics between both systems based on an insular-dependent system.

Objectives
In this study, we examined the effects of a single dose of nalmefene on resting-state functional connectivity (rsFC) patterns within and between these addiction-related neural systems in AUD.

Methods
Non-treatment seeking participants with AUD (N = 17; 19–66 years, 6 female) took part in a randomized, placebo-controlled, double-blind, crossover study and received either a single dose of 18 mg nalmefene or a placebo. Using seed-based correlation analyses on resting‐state functional magnetic resonance imaging data, we examined the effects of nalmefene on key nodes related to the (1) impulsive system; (2) reflective system; (3) salience network; and (4) default mode network.

Results
Under nalmefene, participants showed reduced rsFC between components of the impulsive system (Nucleus accumbens–putamen/pallidum/insula). Reduced rsFC was found between elements of the reflective system and impulsive system (orbitofrontal cortex–insula/putamen/pallidum), salience network (orbitofrontal cortex–insula/inferior frontal gyrus), and default mode network (lateral prefrontal cortex–precuneus/cuneus). Components of the salience network showed both increased (anterior cingulate cortex) and decreased (insular cortex) rsFC to elements of the reflective system.

Conclusion
A single dose of nalmefene impacts rsFC and alters the interaction between key nodes of addiction-related neural systems in non-treatment seeking participants with AUD. Nalmefene may normalize rsFC patterns by weakening the impulsive system while strengthening the reflective system.
Trial registration: clinicaltrials.gov: NCT02372318.
Are reaching and grasping effector-independent? Similarities and differences in reaching and grasping kinematics between the hand and foot
Authors: Liu Yuqi , Caracoglia James , Sen Sriparna , Freud Erez , Striem-Amit Ella 
Published: April 2022

AbstractWhile reaching and grasping are highly prevalent manual actions, neuroimaging studies provide evidence that their neural representations may be shared between different body parts, i.e., effectors. If these actions are guided by effector-independent mechanisms, similar kinematics should be observed when the action is performed by the hand or by a cortically remote and less experienced effector, such as the foot. We tested this hypothesis with two characteristic components of action: the initial ballistic stage of reaching, and the preshaping of the digits during grasping based on object size. We examined if these kinematic features reflect effector-independent mechanisms by asking participants to reach toward and to grasp objects of different widths with their hand and foot. First, during both reaching and grasping, the velocity profile up to peak velocity matched between the hand and the foot, indicating a shared ballistic acceleration phase. Second, maximum grip aperture and time of maximum grip aperture of grasping increased with object size for both effectors, indicating encoding of object size during transport. Differences between the hand and foot were found in the deceleration phase and time of maximum grip aperture, likely due to biomechanical differences and the participants’ inexperience with foot actions. These findings provide evidence for effector-independent visuomotor mechanisms of reaching and grasping that generalize across body parts.
MRI signal and morphological alterations of the suprapatellar fat pad in asymptomatic subjects: are these normal variants?
Authors: Cosentino Aurelio , Richard Raphaël , Baron Margaux , Demondion Xavier , Favre Julien , Omoumi Patrick 
Published: April 2022

AbstractObjectiveTo study the prevalence of suprapatellar fat pad (SPFP) MR alterations in asymptomatic subjects, in relation to a wide range of clinical/imaging parameters, including muscle performance tests and physical activity data.Materials and methodsWe prospectively included 110 asymptomatic subjects as part of a cohort study. Inclusion criteria were no knee pain in the last year. Exclusion criteria were any medical/surgical history of a knee disorder. Subjects underwent knee and low-dose posture radiographs [EOS®], 3 T MRI, clinical examination including muscle performance tests, and physical activity monitoring. The presence/absence of SPFP alterations (hyperintensity and mass effect) were assessed through consensus reading on fluid-sensitive sequences. Differences between groups of knees with SPFP alterations and controls were tested for a total of 55 categorical/continuous clinical/imaging parameters, including SPFP relative-T2-signal, trochlear/patellar/lower-limb morphologic measurements. Wilcoxon-rank-sum and chi-square tests were used to compare groups of patients. The histological correlation was obtained in a cadaveric specimen.ResultsSPFP alterations were common in asymptomatic subjects: hyperintensity 57% (63/110) and mass effect 37% (41/110), with 27% (30/110) showing both. Among the 55 imaging, clinical, or activity parameters tested, only increased patellar tilt angle (p = 0.02) and TT-TG distance (p = 0.03) were statistically different between groups of SPFP alterations and controls. The histological correlation showed more abundant connective tissue in SPFP compared to the prefemoral fat pad.ConclusionsSPFP hyperintensity and mass effect are common MRI findings in asymptomatic knees, and they are not related to most imaging, clinical, and activity parameters. Care should be taken not to overcall them pathological findings as they most likely represent normal variants.
Transition metal dichalcogenide-based Janus micromotors for on-the-fly Salmonella detection
Authors: Pacheco Marta , Jurado-Sánchez Beatriz , Escarpa Alberto 
Published: April 2022

AbstractJanus micromotors encapsulating transition metal dichalcogenides (TMDs) and modified with a rhodamine (RhO)-labeled affinity peptide (RhO-NFMESLPRLGMH) are used here for Salmonella enterica endotoxin detection. The OFF–ON strategy relies on the specific binding of the peptide with the TMDs to induce fluorescence quenching (OFF state); which is next recovered due to selectively binding to the endotoxin (ON state). The increase in the fluorescence of the micromotors can be quantified as a function of the concentration of endotoxin in the sample. The developed strategy was applied to the determination of Salmonella enterica serovar Typhimurium endotoxin with high sensitivity (limits of detection (LODs) of 2.0 µg/mL using MoS2, and 1.2 µg/mL using WS2), with quantitative recoveries (ranging from 93.7 ± 4.6 % to 94.3 ± 6.6%) in bacteria cultures in just 5 min. No fluorescence recovery is observed in the presence of endotoxins with a similar structure, illustrating the high selectivity of the protocol, even against endotoxins of Salmonella enterica serovar Enteritidis with great similarity in its structure, demonstrating the high bacterial specificity of the developed method. These results revealed the analytical potential of the reported strategy in multiplexed assays using different receptors or in the design of portable detection devices.
Graphical abstract
Strukturqualität im Berliner Notarztdienst: Funktionen, Qualifikationen und Kompetenzerhalt
Authors: Breuer Florian , Dahmen Janosch , Malysch Tom , Poloczek Stefan 
Published: April 2022

ZusammenfassungDie Voraussetzungen zum Einsatz im Notarztdienst sind in Deutschland unterschiedlich geregelt, allein die Rahmenbedingungen zur Erlangung der Zusatzbezeichnung Notfallmedizin variieren in den Bundesländern. Diese stellt nur die Mindestvoraussetzung zur ärztlichen Teilnahme am Rettungsdienst dar und ist nach aktueller Literatur und Rechtsprechung, ebenso wie die alleinige Teilnahme am Notarzt- oder Klinikdienst zum Kompetenzerhalt, längst nicht mehr ausreichend. Im Rahmen der erneuten Ausschreibung des Notarztdiensts im Land Berlin im Jahr 2020 wurden die Voraussetzungen zur Teilnahme am Notarztdienst an den Stand der medizinischen Wissenschaft und aktuellen Rechtsprechung angepasst, die Definition von ärztlichen Kompetenzniveaus und deren strukturierter Erwerb inklusive festgelegter Einarbeitungs- und Einsatzzeiten entsprechend definiert sowie supervidierende Komponenten festgelegt. Bereits existierenden ärztlichen Führungsfunktionen wie dem Oberarzt vom Dienst (OAVD) kommt als operativer Vertreter der Ärztlichen Leitung Rettungsdienst in der Notfallrettung der Hauptstadt eine zentrale Rolle bei der Etablierung und Sicherung dieser gebotenen Anpassungsmaßnahmen zu.
Hume on the Monetary Fallacy of Monotonic Counterfactuals
Authors: Maffezioli Paolo 
Published: April 2022

AbstractI focus on the commonly shared view that Hume’s monetary theory is inconsistent. I review several attempts to solve the alleged inconsistency in Hume’s monetary theory, including the consensus interpretation according to which Hume was committed to the neutrality of money only in the long run, while he conceded that money can be non-neutral in the short run. Then, building on a monetary version of the logical fallacy of monotonic counterfactuals in the essay Of the Balance of Trade, I argue for the consistency of Hume’s theory of money by ascribing to Hume a distinction between money as collectively neutral and distributively non-neutral.
Driving state evaluation of intelligent connected vehicles based on centralized multi-source vehicle road collaborative information fusion
Authors: Tong Qiuhong , Yang Zhuolin , Chai Guoqong , Wang Yijie , Qi Zihao , Wang Feng , Yin Kangchao 
Published: April 2022

AbstractWhen the intelligent vehicle is driving on the road, the driving state of the vehicle needs to be monitored and evaluated in real time, such as whether the distance from the vehicle or obstacle in front of it is often less than or close to the safe driving distance, whether it often deviates from the lane at high speed, whether the temperature of the power battery is often higher or lower, etc. The intelligent vehicle has rich environment perception systems and communication systems. A large amount of effective driving state information is obtained through these systems, then the information is fused by intelligent algorithm, which is very useful to evaluate the driving state. By using the developed Intelligent Connected Vehicle (ICV) information collection system platform, including vision, radar, environmental perception, positioning, and V2X communication system for obtaining roadside information, etc. various driving state parameters of the vehicle during the current driving process of the intelligent vehicle are obtained in real time when the vehicle is driving, then transmitted to the monitoring platform, and the intelligent immune algorithm is applied to establish model on monitoring platform. In the model, the state space representation of related problems is designed. The user establishes the autologous library according to the typical vehicle driving state in the monitoring road driving characteristics, which is used as the known antigen. The parameters of the real-time driving state of the vehicle are used as the unknown antigen, and the immune algorithm model is applied to get the evaluation of the current vehicle driving state and feedback the evaluation results to the vehicle and give early warning. In this paper, the model is verified by experiments, and the data are analyzed. In the experiment, the evaluation results of the monitoring platform are consistent with the state of the driving vehicle, which shows the feasibility of this model for the evaluation of the driving state of the ICV.
Quantification of adaptive forces on SNP rs1010211 due to viral zoonotic pathogens
Authors: Alsufyani Daniah , Lindesay James 
Published: April 2022

AbstractWidespread genotyping of human populations in environmental homeostasis has created opportunities to quantify how environmental parameters affect the genomic distribution of variants in healthy populations. This represents an ongoing natural experiment upon the human species that can only be understood through developing models of adaptation. By examining the information dynamics of optimal SNP distributions within such populations, “adaptive forces” on genomic variants can be quantified through comparisons between different populations. To this end, we are performing double-blind scans of SNPs in order to ascertain any potential smooth functional relationships between the frequencies of the variants and changes in quantified environmental parameters. At present, we have sequentially examined more than twenty thousand SNPs (on chromosome 3) of nine homeostatic native populations for potential adaptive flagging of the variants as functions of 15 environmental parameters. Our first significant flag has related rs1010211 to viral pathogens in mammalian hosts. Such pathogens present a significant risk for the emergence of new infectious diseases in humans. This genomic variant is within the gene TNIK, which is a germinal center kinase (GCK). GCKs are participants in both adaptive and innate immune regulation. However, the function of TNIK is not fully understood. We quantify the adaptive force on the C allele due to the pathogens as 0.04 GEU’s/viral species.