bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synovial fluid evaluation in COVID-19 patients
Authors: Jainal Arifin  , Jainal Arifin  , Muhammad Nasrum Massi  , Yosua Adi Nugroho  , Mirza Ariandi  , Yosia Handoko  
Published: December 2022

Background: COVID-19 can provide symptoms outside the respiratory tract and musculoskeletal symptoms. Some viruses can indeed cause arthritis, and the genetic material of these viruses can be found in a joint fluid using PCR assays. With this study, we will conduct an examination using PCR on the joint fluid of COVID-19 patients who have musculoskeletal symptoms, especially arthralgia, during the infection.
Methods: This cross-sectional study with a descriptive explanation of synovial fluid in COVID-19 patients was performed from April 1st to July 30th, 2021, with patient data collection conducted centrally at Wahidin Sudirohusodo Hospital Makassar Synovial fluid was taken from the knee using the sterile procedure in a patient that met the inclusion criteria, such as hospitalized patients who had positive PCR swab results in the nasopharynx for less than 2 weeks and had arthralgia symptoms on April – July 2021. Other musculoskeletal symptoms (e.g., myalgia and fatigue) were noted. About 2 ml sample of synovial fluid was tested using a PCR kit (M BioCov) in the laboratory. The sample shows a positive result when there is an amplification of RDNP with a CT value below 40.
Results: 189 patients with 21 participated, 14 subjects were male, and 7 subjects were female. Age range 18 - 72 years with arthralgia, myalgia, and fatigue symptoms. Only 2 patients had arthralgia, 13 patients had myalgia and arthralgia, 1 patient had fatigue and arthralgia, and 5 patients had all of the symptoms (arthralgia, myalgia, and fatigue). Only 1 patient had direct contact with the COVID-19 patient. PCR test for synovial fluid in all patients showed negative for COVID-19.
Conclusion: COVID-19 infection can be manifest in the musculoskeletal system. Detection of COVID-19 virus using a specimen from knee synovial fluid in a patient with musculoskeletal symptoms (arthralgia, myalgia, fatigue) was negative.
Drop foot and adult onset tethered cord syndrome caused by filum terminal ependymoma: A case report
Authors: Wardhana Dewa Putu Wisnu , Permana Made Agung Yudistira , Awyono Steven , Maliawan Rani Paramitha Iswari , Novita  , Rosyidi Rohadi Muhammad 
Published: December 2022

Introduction: Filum terminal is a fibrous tissue to stabilize the position of the spinal cord, located in both intradural and extradural sections anchored caudally to the coccyx. Tumors located at the filum terminal are a rare case. Filum terminal does not have any spinal cord component, but tumours in that area can directly cause a significant neurological disorder.
Case Presentation: Presenting 43 years old man with chief complaints of drop left foot and tethered cord syndromes. MR Image revealed 9,4x10mm intradural extramedullary mass at L1 with L1-L5 disc desiccation. The patient was untethered by laminectomy and underwent total mass excision. The sample was analyzed pathologically, and the results matched myxopapillary ependymoma. Pain sensation and sensory disturbance immediately improved after surgery. The urinary disorder also gradually improved. The patient was discharged without any urinary problems. After six months of follow-up, painless, mild left lower drop foot and mild gait disturbance was found without residual urinary and other neurological disorder.
Conclusion: Filum terminal ependymomas need to be considered for differential diagnoses in patients with drop foot and adult-onset tethered cord syndromes.
Protective effect of lavender essential oils on depression and multi-organ stress
Authors: Algristian Hafid , Bintarti Tri Wahyuni , Baroroh Rani Nur Mukaromatim , Leila Qorieatul , Ulfa Reina , Amelia Krismawati  , Nurdiana Mifa , Sudjarwo Giftania Wardhani , Nurhidayat Adhi Wibowo , Irawan Satriotomo  , Handajani Retno 
Published: December 2022

Introduction: Lavender essential oils (LEO) have been known to have relaxing effects, improve mood, and treat anxiety, but the effect on multiple organ stress concurrently is unknown. This multiorgan stress is related to depression can be caused by chronic psychological stress due to excessive cortisol levels and can lead to organ damage. This study analyzed LEO in preventing depression and multiorgan failure using intraperitoneal injection of corticosteroids in animal-model.
Methods: Rats (Rattus norvegicus) Wistar strain, male, aged 7-8 weeks were involved in this study. Depression in animal-model is defined by immobilization using tail suspension test and anhedonia using sucrose preference test. LEO 5% was diluted in virgin coconut oils as vehicle. Serum cortisol was analyzed using enzyme-linked immunosorbent assay (ELISA). Organs were extracted and processed using hematoxylin eosin staining.
Results: The results of this study indicate that LEO was able to prevent damage to the glial cells, myocardiac cells, and gastrointestinal mast cells infiltration, but not to the hepatocytes and renal cells damage. LEO also induced behavioral activation as improvement of depression, but anhedonia was still remained.
Conclusion: The effect of LEO is to prevent the increase in blood cortisol levels, thus reduce the reactivity of depressed individuals to stress, although the individual still has anhedonia as a residual symptoms
Antibacterial activity of Sentul fruit peel extract (Sandoricum koetjape) against Streptococcus mutans and Staphylococcus aureus
Authors: Wirata I Nyoman , Anak Agung Gede Agung  , Ni Wayan Arini  , Regina Tedja Sulaksana  , Mochammad Choirul Hadi  , I Gusti Ayu Raiyanti  
Published: December 2022

Introduction: Infectious mouth diseases are caused by microorganisms such as Staphylococcus aureus and Streptococcus mutans. Sentul fruit peel extract contains several phytochemical compounds, flavonoid compounds, saponins and tannins are aromatic hydroxyl groups that act as antibacterial so that they can be used as a treatment for infections of the mouth. This study aimed to determine the inhibitory ability of Sentul fruit peel extract (Sandoricum koetjape) against Streptococcus mutans and Staphylococcus Aureus bacteria which can cause oral infections.
Method: Sentul fruit peel simplicia was extracted by maceration method with ethanol for 24 hours. The maceration method was chosen in this study because it is a method that is easy to do and uses simple tools, which is enough to soak the sample in a solvent. A filtering process followed this and the filtrate was then evaporated with a vacuum rotary evaporator at a temperature of 45oC, so that a thick extract was produced. The maceration process was repeated 2 times. After the extraction process, then proceed with liquid-liquid fractionation using distilled water. The thick extract was put into a separating funnel and distilled water was added. Then it was shaken and the aquadest fraction was taken, followed by evaporation with a vacuum rotary evaporator at 45oC, so that the aqua fraction of the ethanol extract was produced.
Result: The results showed that the Sentul fruit peel extract was able to inhibit the growth of S. mutans and S. aureus bacteria as seen from the formation of a clear zone. Based on the ANOVA test, the extract concentration treatment had a significant effect (p<0.005) on the diameter of the clear zone on S. mutans and S. aureus bacteria. Positive control (streptomycin) showed significant differences in Duncan's test, because it produced the greatest antibacterial activity against test bacteria compared to negative control and various extract concentrations. The diameter of the clear zone in the positive control against S. mutans and S. aureus bacteria were 17.63±0.28 mm and 17.62±1.04 mm, respectively. Sentul peel ethanol extract of aqua fraction at a concentration of 100% gave the highest inhibition zone compared to concentrations of 75%, 50%, and 25%, although it was still smaller than the diameter of the clear zone in the positive control. In bacteria S. mutans, the ethanol extract of Sentul peel aqua fraction at a concentration of 100% gave a clear zone diameter of 14.31±1.06 mm and in S. aureus bacteria it gave a clear zone diameter of 15.34±1.81 mm.
Conclusion: Sentul fruit peel extract (Sandoricum koetjape) has the ability to inhibit Streptococcus mutans bacteria and Staphylococcus Aureus bacteria.
The impact of age, gender, family history of allergy, clinical symptoms, and duration of illness on flare diameter of skin prick test
Authors: Wardana Uuk Riuh Kusuma , Endaryanto Anang , Prakoeswa Cita Rosita Sigit 
Published: December 2022

Introduction: Skin prick test (SPT) is a method for diagnosing IgE-mediated allergen sensitization. Although the sensitivity and specificity are quite good, false positive and false negative results are possible. The diameter of the wheal and flare of each allergen can affect the skin prick test measurement, especially if the results of the examination overlap. Because of the usage of the SPT and the emphasis on quality control of health services recently, several variables that can affect the results of SPT must be considered. This study aims to analyze the effect of age, gender, family history of allergy, clinical symptoms, and illness duration on SPT's flare diameter.
Method: This is a cross-sectional study conducted from 2015 to 2020 in Surabaya, Indonesia. Children with possible allergies were tested with a skin prick test at the allergy immunology outpatient clinic of the Dr. Soetomo General Academic Hospital. Inclusion criteria were ≤ 18 years old children, possibly diagnosed with allergies, who had skin prick test. Children with incomplete medical record data were excluded. The Chi-square test or Fisher exact test to determine the relationship between the independent and dependent variables for nominal data. The p-value was declared significant if P<0.05 to determine the effect between variables on flare diameter on skin prick test. We use SmartPLS to analyse the relationships simultaneously to compare relationships with the regression analysis.
Result: Age, gender, clinical symptoms, and duration of illness did not show a significant effect. In contrast, the flare diameter determined by SPT and the presence of an allergic family had a significant correlation (p = 0.017). Therefore, the appropriate distance between allergens must be specified to prevent overlap of SPT flare diameters.
Conclusion: Member of family allergy affects the diameter of the flare on skin prick test.
Comparison of Protein-C levels in diabetes melitus type 2 patients with and without ulcers at Haji Adam Malik Hospital Medan Indonesia May-July 2017
Authors: Zulfa Zulfahmi , Mardia Andri I , Handayani Savita , Syafril Santi , Gatot Dairion 
Published: December 2022

Introduction People with diabetes mellitus (DM) have an increased risk for thrombosis compared with non-diabetic patients. Several studies showed contradicting in data on levels of protein C in people with type 2 DM and diabetic ulcers.Methods This is a descriptive and analytical cross-sectional study on protein C concentration of patients with type 2 DM with and without foot ulcers. The control group was the type 2 DM patients without foot ulcers, whereas the case group was the type 2 DM patients with a rigid diabetic foot ulcer based on the Wagner criteria. Blood samples are then taken after 8 to 10 hours of fasting to check for protein C concentration and hemostasis examination including platelet count, prothrombin time (PT),Â activated partial thromboplastin time (aPTT), thrombin time (TT), fibrinogen levels, and D-dimers.Results Of a total of 20 samples, there was no statistically significant difference in protein C levels of diabetic foot ulcers with hypercoagulation compared with no diabetic foot ulcers with hypocoagulation and no significant correlation between grade diabetic foot ulcers and protein C concentration.
HER2/neu and Ki-67 as prognostic factors in serous type ovarian carcinoma
Authors: Dewi I Gusti Ayu Sri Mahendra , Susraini Anak Agung Ayu Ngurah , Ekawati Ni Putu 
Published: December 2022

Introduction: Serous type ovarian carcinoma occurs in about 59% of all ovaryâ€™s malignant tumors. The high incidence of advanced ovarian carcinoma at the time of diagnosis causes 5-year survival rate of only about 20%. Thus deep science in predictive biomarkers of prognosis is needed, which later could use in therapeutic considerations. Human epidermal growth factor receptor-2 (HER2/neu) is a proto-oncogene that is involved in cell proliferation, differentiation, and apoptosis. Ki-67 is a marker of proliferative activity that plays an essential role in tumor aggressiveness. This study aimed to prove that HER2/neu and Ki-67 have a role as a prognostic factor in serous type ovarian carcinoma.Methods: The study design was an analytic cross-sectional study, with total sample were 36 samples from the patientâ€™s surgery specimen of serous type ovarian carcinoma, examined at Anatomical Pathology Laboratory Faculty of Medicine, Universitas Udayana/Sanglah General Hospital, Denpasar. Histopathological diagnosis for the type of malignancy, grade, and pathological stage according to tumor size was determined on H & E stain. The expression of HER2/neu and Ki-67 was examined with immunohistochemical stain. The correlation between HER2/neu and Ki-67 expression with the degree of differentiation and tumor size was analyzed by the chi-square test, with p<0.05 was significant.Result: The mean of the age was 49.6 Â± 11.28, with range was between 26-64 years. Â The most were in the age group 51-60 years (38.9%). No significant correlation was found between HER2/neu expression with the degree of differentiation (Î± =0.178) and tumor size (Î± =0.264). A significant correlation was found between Ki-67 expression with the degree of differentiation (Î± =0.019) and tumor size (Î± =0.039).Conclusion: In this study, Ki-67 can be considered for its role as a prognostic factor, whereas HER2/neu requires further research, with a larger sample and followed by an examination of gene amplification.
IDH1 mutation in Balinese glioma patients and its relationship with clinicopatological parameters
Authors: Sriwidyani Ni Putu , Wahyuniari Ida Ayu Ika , Saputra Herman , Arijana I Gusti Kamasan Nyoman 
Published: December 2022

Purpose: Isocitrate dehydrogenase 1 (IDH1) mutation plays an important role in the carcinogenesis of gliomas. The most recent WHO classification of central nervous system tumors has added molecular genetic in addition to the histological features of the tumor, including status of IDH mutation in glial tumors. The purpose of this study was to determine the prevalence of IDH1 mutations in Balinese glioma patients and its association with clinicopathological features.Patients and methods: This study was conducted from 30 glioma patients at Sanglah General Hospital during January 2018 until June 2019. DNA extractions were carried out from the FFPE of tumor tissue, followed by amplification of codon 132 in exon 4 of the IDH1 gene by allele specific PCR. Relationship between IDH1 mutation status and clinicopathological features was tested with X2 with 0.05 significance.Results: The age range of patients is 14-81 years with an average age of 43.4 years (SDÂ±17.9 years). Most patients with astrocytic tumors (25/30; 83.3%), others were oligodendroglial tumor (2/30; 6.7%) and oligoastrocytic tumor (3/30; 10%). Most patients were found with grade IV glioblastoma (18/30; 60%).Â Genotyping analysis showed IDH1 mutations in the majority of glioma patients (90%). Most cases (92.6%) of mutant IDH1 showed heterozygous mutations (GA genotype) while the rest showed homozygous mutations (AA genotype). There were no significant relationship between IDH1 status and age (p=0.09), sex (p=0.63) and histologic type (0.14), but there was significant relationship between IDH1 mutation status and tumor grade (p=0.01). All of IDH1 mutation were found in diffuse glioma (grade II and III gliomas and grade IV glioblastoma).Conclusion: Most of diffuse glioma (grade II-IV glioma) patients in Bali had IDH1 mutation and IDH1 mutation status has significant relationship with tumor grade. Further research about genetic abnormalities in order to improve therapeutic efficacy against IDH-mutated gliomas is needed.
HIV-related toxoplasma encephalitis: a tertiary care experience
Authors: Prabowo Kurniawan Adi , Gayatri Anak Agung Ayu Yuli , Sukmawati Ni Made Dewi Dian , Utama Made Susila , Somia Ketut Agus , Merati Ketut Tuti Parwati 
Published: December 2022

Introduction: Toxoplasma encephalitis (TE) is the most commonly found in HIV patients who have no cotrimoxazole prophylaxis, especially when the CD count is <100 / µL. Therapy is often based on a presumptive diagnosis because definitive diagnosis is difficult, and TE can be life threatening. Aim of this study is to provide a characteristic description of TE in HIV patients who admitted at Sanglah Hospital.
Methods: This is a retrospective descriptive study of 43 adult patients admitted with diagnosis TE at the Sanglah Hospital, Indonesia between January 1st 2018 to December 31th 2018.
Results: Most patients were male (69.8%), with the predominant age ranged 31-40 years old (39.5%). Among all patients; 14% on cotrimoxazole prophylaxis, and 23.3% on antiretroviral therapy. The most common clinical manifestation was headache (58.1%). Oral candidiasis was found in 67.4% of patients. CD4 levels <100 cells / µL were found in 62.8% of patients, absolute lymphocytes <500 / µL in 32.6% of patients. Anemia was found in 37.2% of patients. Multiple ring enhancement was the most common feature found on head CT scan, with the profound location was parietal lobe (46.5%). Pyrimethamine + clindamycin is used in 72.1% of cases. The TE mortality rate in HIV patients in this study was 11.6%.
Conclusion: TE in HIV patients was more common on male, younger age and who are not receiving appropriate prophylaxis and/or without antiretroviral therapy. Laboratory study predominantly showed severe immunodeficiency. The most patients making a full recovery with pyrimethamine and clindamycin treatment.
BODY HEIGHT INCREASED OF NEW STUDENT OF UDAYANA UNIVERSITY 1990 AND 2000
Authors: Arya Sena I Gede , Suyasning HI , Sutjana IDP 
Published: December 2022

Changes in body height have been affected by many factors among others genetic,health, hormonal, social economic, exercise, nutrition intake, etc. It was due toimportance physiology parameter to determine growth and nutrition conditioned of thepeople. Income per capita in West Countries higher than Asian includes Indonesian.Some approximates of mean the body height were done on Norwegian and USA isincreasing, up to 13 mm and 10 mm until ten years. Further, the assumption of nutritionintake is increasing for mothers and infant, unanimously increasing of Bali (Indonesian)gross domestic product until 1975 - 1980. So that body height has been increased to nextgeneration. A total of 3.917 subject on 1990, divided in to 2.340 male and 1.577 femaleÂ students and 1.218 subject on 2000 divided in to 525 male and 693 female students. Theresult showed that body height has been increased for male and female 22,1 mm; 19.88mm, respectively.
PEMBELAJARAN SAINS MELALUI PENDEKATAN ERGONOMI MENGURANGI KELUHAN MUSKULOSKELETAL, KEBOSANAN DAN KELELAHAN SERTA MENINGKATKAN MOTIVASI DAN PRESTASI BELAJAR SISWA SD 1 SANGSIT KECAMATAN SAWAN KABUPATEN BULELENG
Authors: Wijana Nyoman , Nala I Gusti Ngurah , Tirtayasa I Ketut , Sutajaya I Made 
Published: December 2022

Tujuan penelitian ini adalah untuk mengetahui peranan pendekatan ergonomidalam pembelajaran sains antara kelompok kontrol dengan kelompok eksperimenPeranan tersebut dalam hal (1) penurunan keluhan muskuloskeletal siswa SD; (2)penurunan kebosanan; (3) penurunan kelelahan siswa SD; (4) peningkatan motivasibelajar sains; dan (5) peningkatan prestasi belajar sains. Jenis penelitian ini termasukjenis penelitian quasi eksperimental dengan rancangan randomized pre-test and posttestcontrol group design. Pada kelompok kontrol pembelajarannya dengan carakonvensional (tanpa PE) dan kelompok eksperimen dengan PE. Pengambilan sampeldilakukan secara random. Analisis data dilakukan dengan menggunakan uji Mann-Whitney dan uji t Group dengan taraf signifikansi 5 %. Simpulan dari penelitian iniadalah (1) pendekatan ergonomi dapat menurunkan keluhan muskuloskeletal siswa SD,baik akibat penggunaan meja belajar dan tempat duduk siswa serta akibat penggunaantas punggung, antara kelompok kontrol yang dalam pembelajarannya tanpamenggunakan pendekatan ergonomi (PE) dengan kelompok eksperimen yang dalampembelajarannya dengan menggunakan PE (p < 0,05). Penurunan keluhanmuskuloskeletal akibat penggunaan meja dan tempat duduk siswa sebesar 42,54(99,88%) dan penurunan keluhan muskuloskeletal akibat penggunaan tas punggungsebesar 47,97 (99,83%); (2) pendekatan ergonomi dapat menurunkan kebosanan siswaSD, antara kelompok kontrol yang dalam pembelajarannya tanpa menggunakanpendekatan ergonomi (PE) dengan kelompok eksperimen yang dalam pembelajarannyadengan menggunakan PE (p < 0,05). Penurunan kebosanannya adalah sebesar 18,73(26,40%); (3) pendekatan ergonomi dapat menurunkan kelelahan siswa SD, antarakelompok kontrol yang dalam pembelajarannya tanpa menggunakan pendekatanergonomi (PE) dengan kelompok eksperimen yang dalam pembelajarannya denganmenggunakan PE (p < 0,05). Penurunan kelelahannya adalah sebesar 30,78 (73,76%);(4) pendekatan ergonomi dapat meningkatkan motivasi belajar sains pada siswa SD,antara kelompok kontrol yang dalam pembelajarannya tanpa menggunakan pendekatanergonomi (PE) dengan kelompok eksperimen yang dalam pembelajarannya denganmenggunakan PE (p < 0,05). Peningkatan motivasi belajar siswa adalah sebesar 14,65(65,81%); (5) pendekatan ergonomi dapat meningkatkan prestasi belajar dalam bidangsains pada siswa SD, antara kelompok kontrol yang dalam pembelajarannya tanpamenggunakan pendekatan ergonomi (PE) dengan kelompok eksperimen yang dalampembelajarannya dengan menggunakan PE (p < 0,05). Peningkatan prestasi belajarsiswa di bidang sains adalah sebesar 12,72 (33,70%). Dapat disarankan bahwaÂ pembenahan di dunia pendidikan hendaknya tidak bersifat partial akan tetapi dilakukansecara multidisipliner.