bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel method of ancient pottery analysis based on radioactive isotope ratios: a pilot study
Authors: Długosz-Lisiecka Magdalena , Sikora Jerzy , Krystek Marcin , Płaza Dominik , Kittel Piotr 
Published: July 2022

Abstract
The proposed method has been applied to a multi-phased settlement complex and pottery manufacturing centre in Ostrowite in northern Poland. In this study the radioactive isotope ratios method has been applied to a set of ceramic pottery specimens from the same multi-layered archaeological site and probably produced from local raw material. The pilot radiometric research was based on: 24 Neolithic (5200–5000 BCE), 21 Early Iron Age (800–600 BCE), 4 Roman Period (100–400 CE), and 13 Middle Ages (1030–1320 CE) pottery fragments, and also 8 samples of local clay (glacial till) and 3 burned clay samples. The method shows the similarities within the ceramic material used to manufacture the pottery. The variations in the quantitative and qualitative compositions of the basic products (clay, silt, loam, sand, ash and organic admixtures) used in the preparation of the ceramic paste change the isotopic composition and activity ratios. Pottery from each ceramic manufacturing centre, based on the specific composition of the raw materials, have characteristic isotope ratios. Radioactive isotope ratios as fingerprints of ancient ceramic manufacturing centres have not yet been applied as an archaeometric method. In this study two isotope ratios have been selected and applied: 40K/228Ac and 226Ra/208Tl. All analysis have been done using gamma spectrometry system, with quality and quantity analysis of the spectrums. Low-background passive shield was used for obtain improvement of results quality. The pilot study confirms the grouping of isotope ratio results for each sample type, even in terms of similarities with the base clay material collected in this region.
Prediction and validation of short-to-long-term earthquake probabilities in inland Japan using the hierarchical space–time ETAS and space–time Poisson process models
Authors: Ogata Yosihiko 
Published: July 2022

AbstractA hierarchical space–time version of the epidemic-type aftershock sequence (HIST–ETAS) model was constructed for an optimally adapted fit to diverse seismicity features characterized by anisotropic clustering as well as regionally distinct parameters. This manuscript validates this elaborate model for short-term prediction based on several years of recent inland Japan earthquakes as a testing data set, by evaluating the results using a log-likelihood ratio score. To consider intermediate- and long-term performance, several types of space–time Poisson models are compared with the background seismicity rate of the HIST–ETAS model. Results show first that the HIST–ETAS model has the best short-term prediction results for earthquakes in the range of magnitudes from M4.0 to M5.0, although, for the larger earthquakes, sufficient recent earthquake data is lacking to evaluate the performance. Second, for intermediate-term predictions, the optimal spatial nonuniform Poisson intensity model has a better forecast performance than the seismic background intensity of the HIST–ETAS model, while the uniform rate Poisson model throughout all of inland Japan has the worst forecast performance. For earthquakes of M6 or larger, the performance of retrospective long-term forecasts was tested in two ways. First, a retrospective forecasting experiment divided the entire period from 1885 to the present into two parts, with the recent ~ 30 years as the forecast period. Second, the historical damaging earthquake data (599–1884) were spatially validated using century data from 1885 to the present. In both validations, it was determined that the spatial intensity of the inland background seismic activity of the HIST–ETAS model is much better than the best-fit nonuniform Poisson spatial model, leading to the best results. The findings of this study will be critical for regional earthquake hazard planning in Japan and similar locations worldwide.
Graphical Abstract
A decellularized flowable placental connective tissue matrix supports cellular functions of human tenocytes in vitro
Authors: Mao Yong , John Nikita , Protzman Nicole M. , Kuehn Adam , Long Desiree , Sivalenka Raja , Junka Radoslaw A. , Gosiewska Anna , Hariri Robert J. , Brigido Stephen A. 
Published: July 2022

Abstract
Purpose
Injectable connective tissue matrices (CTMs) may promote tendon healing, given their minimally invasive properties, structural and biochemical extracellular matrix components, and capacity to fill irregular spaces. The purpose of this study is to evaluate the effects of placental CTMs on the cellular activities of human tenocytes. Decellularization, the removal of cells, cell fragments, and DNA from CTMs, has been shown to reduce the host’s inflammatory response. Therefore, the authors hypothesize that a decellularized CTM will provide a more cell-friendly matrix to support tenocyte functions.

Methods
Three human placental CTMs were selected for comparison: AmnioFill® (A-CTM), a minimally manipulated, non-viable cellular particulate, BioRenew™ (B-CTM), a liquid matrix, and Interfyl® (I-CTM), a decellularized flowable particulate. Adhesion and proliferation were evaluated using cell viability assays and tenocyte migration using a transwell migration assay. Gene expression of tenocyte markers, cytokines, growth factors, and matrix metalloprotease (MMP) in tenocytes were assessed using quantitative polymerase chain reaction.

Results
Although A-CTM supported more tenocyte adhesion, I-CTM promoted significantly more tenocyte proliferation compared with A-CTM and B-CTM. Unlike A-CTM, tenocyte migration was higher in I-CTM than the control. The presence of I-CTM also prevented the loss of tenocyte phenotype, attenuated the expression of pro-inflammatory cytokines, growth factors, and MMP, and promoted the expression of antifibrotic growth factor, TGFβ3.

Conclusion
Compared with A-CTM and B-CTM, I-CTM interacted more favorably with human tenocytes in vitro. I-CTM supported tenocyte proliferation with reduced de-differentiation and attenuation of the inflammatory response, suggesting that I-CTM may support tendon healing and regeneration in vivo.
Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid
Authors: Ma Yue , Li Tianzhen , Tan Zijian , Ma Long , Liu Haifeng , Zhu Leilei 
Published: July 2022

AbstractCatalytic valorization of raw glycerol derived from biodiesel into high-value chemicals has attracted great attention. Here, we report chemoenzymatic cascade reactions that convert glycerol to lactic acid and glycolic acid. In the enzymatic step, a coenzyme recycling system was developed to convert glycerol into 1,3-dihydroxyacetone (DHA) with a yield of 92.3% in potassium phosphate buffer (300 mM, pH 7.1) containing 100 mM glycerol, 2 mM NAD+, 242 U/mL glycerol dehydrogenase-GldA and NADH oxidase-SpNoxK184R at 30 °C. Subsequently, NaOH or NaClO2 catalyzes the formation of lactic acid and glycolic acid from DHA. The high yield of lactic acid (72.3%) and glycolic acid (78.2%) verify the benefit of the chemoenzymatic approaches.
Graphical Abstract
Genome sequencing of biocontrol strain Bacillus amyloliquefaciens Bam1 and further analysis of its heavy metal resistance mechanism
Authors: Luo Yuanchan , Chen Lei , Lu Zhibo , Zhang Weijian , Liu Wentong , Chen Yuwei , Wang Xinran , Du Wei , Luo Jinyan , Wu Hui 
Published: July 2022

AbstractPlant growth-promoting rhizobacteria (PGPR) or Biocontrol strains inevitably encounter heavy metal excess stress during the product’s processing and application. Bacillus amyloliquefaciens Bam1 was a potential biocontrol strain with strong heavy metal resistant ability. To understand its heavy metal resistance mechanism, the complete genome of Bam1 had been sequenced, and the comparative genomic analysis of Bam1 and FZB42, an industrialized PGPR and biocontrol strain with relatively lower heavy metal tolerance, was conducted. The comparative genomic analysis of Bam1 and the other nine B. amyloliquefaciens strains as well as one Bacillus velezensis (genetically and physiologically very close to B. amyloliquefaciens) was also performed. Our results showed that the complete genome size of Bam1 was 3.95 Mb, 4219 coding sequences were predicted, and it possessed the highest number of unique genes among the eleven analyzed strains. Nine genes related to heavy metal resistance were detected within the twelve DNA islands of Bam1, while only two of them were detected within the seventeen DNA islands of FZB42. When compared with B. amyloliquefaciens type strain DSM7, Bam1 lacked contig L, whereas FZB42 lacked contig D and I, as well as just possessed contig B with a very small size. Our results could also deduce that Bam1 promoted its essential heavy metal resistance mainly by decreasing the import and increasing the export of heavy metals with the corresponding homeostasis systems, which are regulated by different metalloregulators. While Bam1 promoted its non-essential heavy metal resistance mainly by the activation of some specific or non-specific exporters responding to different heavy metals. The variation of the genes related to heavy metal resistance and the other differences of the genomes, including the different number and arrangement of contigs, as well as the number of the heavy metal resistant genes in Prophages and Genomic islands, led to the significant different resistance of Bam1 and FZB42 to heavy metals.
Graphical Abstract
Axillary schwannoma mimicking lymph node metastasis-associated breast cancer: a case report
Authors: Kumagai Hideki , Takehana Kou , Shioi Yoshihiro , Tono Chihiro 
Published: July 2022

Abstract
Background
Axillary schwannoma associated with breast cancer is an extremely rare disease, and previous reports have been limited. In this setting, there is great concern about whether a tumor in the axillary region is lymph node metastasis. Herein, we report a unique case of axillary schwannoma that mimicked lymph node metastasis associated with breast cancer.

Case presentation
A 68-year-old woman who underwent mastectomy and axillary lymph node dissection for right breast cancer over 20 years ago presented to our hospital with numbness and weakness in the right arm for 6 months. Ultrasonography, computed tomography, and magnetic resonance imaging showed a 20-mm well-circumscribed round tumor in the right axillary region. Initially, she was suspected of having lymph node metastasis-associated breast cancer, but the result of the core needle biopsy was a schwannoma. The patient underwent tumor enucleation. The patient has had no recurrence 1 year after the operation.

Conclusion
Axillary schwannomas often mimic lymph node metastasis in patients with a history of malignancy, particularly breast cancer. To select the optimal treatment, the clinicians should make as accurately as possible a diagnosis, with histopathological examinations, when examining patients with cancer who develop tumors in the axillary region.
Delayed laparoscopic cholecystectomy for a patient with coronavirus disease 2019 who developed gangrenous cholecystitis: a case report
Authors: Yoshida Yurie , Iguchi Tomohiro , Iseda Norifumi , Hirose Kosuke , Honboh Takuya , Iwasaki Noriko , Kato Seiya , Sadanaga Noriaki , Matsuura Hiroshi 
Published: July 2022

Abstract
Background
Gangrenous cholecystitis has a high risk of perforation and sepsis; therefore, cholecystectomy in the early stage of the disease is recommended. However, during the novel coronavirus disease 2019 (COVID-19) pandemic, the management of emergent surgeries changed to avoid contagion exposure among medical workers and poor postoperative outcomes.

Case presentation
A 56-year-old man presented to our hospital with abdominal pain. Computed tomography revealed intraluminal membranes, an irregular or absent wall, and an abscess of the gallbladder, indicating acute gangrenous cholecystitis. Early laparoscopic cholecystectomy seemed to be indicated; however, a COVID-19 antigen test was positive despite no obvious pneumonia on chest computed tomography and no symptoms. After discussion among the multidisciplinary team, antibiotic therapy was started and percutaneous transhepatic gallbladder drainage (PTGBD) was planned for the following day because the patient’s vital signs were stable and his abdominal pain was localized. Fortunately, the antibiotic therapy was very effective, and PTGBD was not needed. The cholecystitis improved and the patient was discharged from the hospital on day 10. One month later, laparoscopic delayed cholecystectomy was performed after confirming a negative COVID-19 polymerase chain reaction test result. The postoperative course was uneventful, and the patient was discharged on postoperative day 2 in satisfactory condition.

Conclusion
We have reported a case of acute gangrenous cholecystitis in a patient with asymptomatic COVID-19 disease. This report can help to determine treatment strategies for patients with gangrenous cholecystitis during future pandemics.
Magnitude and factors associated with iron supplementation among pregnant women in Southern and Eastern Regions of Ethiopia: Further Analysis of mini demographic and health survey 2019
Authors: Mengistu Girma Teferi , Mengistu Bizunesh Kefale , Gudeta Tolesa Gemeda , Terefe Ayana Benti , Habtewold Fedhesa Mamo , Senbeta Mebratu Demissie , Sori Seboka Abebe , Jiru Hirut Dinku 
Published: July 2022

Abstract
Background
Anemia is a global public health problem that affects pregnant women. The most common cause of anemia is iron deficiency which is extremely common in developing countries. World health organization reported that 36.5% of pregnant women are anemic globally. In Ethiopia, 27.08% of women of the reproductive age group are anemic. Therefore, this study aimed to identify the magnitude and factors associated with iron supplementation during pregnancy in the southern and eastern regions of Ethiopia.

Methods
The data used in this analysis were extracted from Mini Demographic and Health Survey 2019. The survey was conducted in 9 regional states and two city administrations. The data used in the analysis were extracted from individual women datasets, and 1780 study participants were included in this study. The logistic regression analysis including bivariate and multivariable logistic regression at a 95% confidence interval and a p-value less than 0.05 was used.

Result
The finding of the study shows that iron supplementation during pregnancy in Southern and Eastern parts of Ethiopia was 50.06%. Among those who received iron, only about 20% took it for 90 days and more during their pregnancy. Iron supplementation among the pregnant women was affected by secondary education [AOR = 2.20, 95%CI (1.325, 3.638)], residing in urban [AOR = 1.75, 95%CI (1.192, 2.574)], having media at home [AOR = 1.41, 95%CI (1.022, 1.946)], having antenatal care follow up [AOR = 9.27, 95%CI (4.727, 18.169)], having 4 and more ANC follow up [AOR = 2.01, 95%CI (1.468,2.760], having antenatal care follow up at government health institutions [AOR = 3.40, 95%CI (1.934, 5.982)], and giving birth at governmental health institutions [AOR = 1.70, 95%CI (1.236, 2.336)].

Conclusion
Only one in two pregnant women was supplemented with iron during their recent pregnancy. The supplementation was affected by women's education, place of residence, presence of media at home, antenatal care follow-up, the number of antenatal care follow up, antenatal care follows up at governmental health institutions, and giving birth at the governmental health institution. The availability and accessibility of maternal care services and their functionality in providing maternal care services improve the supplementation.
UHPLC assessment of embelin in specialized mangrove plant Aegiceras corniculatum (L) Blanco
Authors: Mohapatra Manisha , Basak Uday Chand 
Published: July 2022

AbstractBackgroundEmbelin is one of the biologically charming natural benzoquinones with wide medicament use in therapeutics that is pertained to its unique biochemical structure. Mostly, the plants belonging to Myrsinaceae family especially the fruits of genusEmbeliaare rich source of embelin. However, the colossal use of these plants as an active ingredient in several drug formulations has led toward their decrease population number increasing the threat status. This major issue requires a well-versed scientific approach to search for suitable alternative substitute that can be used as an active embelin source. On this panorama,Aegiceras corniculatum(L.) Blanco can be used as alternate source of embelin. On this aspect, all the plant parts (root, stem bark, leaf and fruit) are being estimated for embelin content both qualitatively and quantitatively using spectrophotometric and ultra-high-performance liquid chromatographic (UHPLC) methods and also asserted through histochemical analysis. All the aforesaid plant parts are extracted through Soxhlet and water bath methods separately and analyzed for embelin content after isolation through column chromatography and thin layer chromatography.ResultsFrom results, it was evaluated that all plant parts showed the presence of embelin in a range of 0.17–1.95% dry wt. through UHPLC method, while the highest content was found in fruit followed by root, bark and leaves. The retention time for embelin was found to be 2.7 min.ConclusionsFrom the above experimentation, both vegetative and reproductive parts ofA. corniculatumhave shown the presence of embelin that can be utilized in ample amount so as to reduce the threat status of its primary source.
Impacts of the transition from family farming to monoculture farming on the eating habits of two cities in the Valle de Tenza, Boyacá—Colombia
Authors: Zambrano Jennifer Lorena Avendaño , da Cruz Denise Dias , de Oliveira Paulino Flavia 
Published: July 2022

AbstractThe Valle de Tenza region, located in the Department of Boyacá—Colombia, shows a transition situation from the family farming of various food crops to coffee farming following an agribusiness model. From this perspective, in order to understand the current scenario of food sovereignty in Guateque and Guayatá, two cities of the Valle de Tenza, this study evaluated socioeconomic, environmental, and cultural aspects based on questionnaires and semi-structured interviews applied to peasant families that practice family farming and/or coffee farming. Moreover, these same aspects were also evaluated among urban food consumers. These evaluations aimed to assess the perception of the interviewees about the availability of regional food crops and current eating habits in relation to those from a decade or more ago, in addition to investigating their knowledge about the traditional cuisine of the region. The cultivation of regional food crops used to prepare local and regional traditional dishes such aspiquete,sancocho, and differentamasijosbased on corn andsagú(Maranta arundinacea) has been significantly reduced. The investigation revealed changes in the eating habits of the Valle de Tenza inhabitants due to the consumption of processed foods and the reduced cultivation of local traditional food crops. As a consequence of this transition to coffee production, the most representative traditional foods are being replaced by more profitable crops, including coffee and some fruits not traditionally grown in the Valle de Tenza and with more local and regional acceptance. This reduction can affect food availability and change the gastronomic and cultural identity of the Valle de Tenza population, among other aspects related to food sovereignty.
Automatic detection of autism spectrum disorder (ASD) in children using structural magnetic resonance imaging with machine vision system
Authors: Khadem-Reza Zahra Khandan , Zare Hoda 
Published: July 2022

Abstract
Background
Autism spectrum disorder (ASD) is a group of developmental disorders of the nervous system whose main manifestations are defects in social interactions, communication, repetitive behaviors, and limited interests. Over the years, the use of magnetic resonance imaging (MRI) to help identify patterns that are common in people with autism has increased for classification purposes. This study propose a method for classifying ASD patients versus controls using structural MRI information. In order to increase the accuracy of this method, the volume and surface features of the structural images are used simultaneously.

Results
The accuracy of diagnosis respectively was 86.29%, 71.15%, 86.53%, and 88.46% with SVM, RF, KNN, and ANN classifiers. The highest accuracy of diagnosis was obtained using ANN.

Conclusions
Since clinical evaluations for the diagnosis of autism are extremely time-consuming and depend on the expertise of a specialist, the importance of intelligent diagnosis of this disorder becomes clear. The aim of this study was to design an intelligent system to diagnose autism spectrum disorder.
Correlation between tumor diameter, distant metastasis site, and survival in extensive stage small cell lung cancer
Authors: Cimen Filiz , Düzgün Sevim , Aloglu Melike , Sentürk Aysegül , Atikcan Sükran 
Published: July 2022

Abstract
Background
Small cell lung cancer (SCLC) is a malignant disease that spreads quickly. There is limited research on the relationship between tumor diameter and distant metastatic patterns in extensive stage small cell lung cancer (ES-SCLC). This study aimed to investigate the relationship between tumor diameter, distant metastasis site, and survival in extensive stage small cell lung cancer.

Method
Patients over the age of 18 who applied to Ankara Atatürk Chest Diseases and Thoracic Surgery Training and Research Hospital with the diagnosis of small cell lung cancer and distant organ metastasis between January 2015 and December 2019 were retrospectively analyzed.

Results
The study comprised a total of 178 patients, with 12 women (6.7%) and 166 men (93.3%) participating. The patient was followed for a period of 1 to 36 months, with a median value of 7 months. The univariate model showed that pancreatic metastasis, single metastasis, tumor diameter, and tumor N stage had a significant (p = 0.003, p = 0.001, p = 0.013, p = 0.001, respectively) effect on survival. The N stage III group’s expected life expectancy [6.8 months (5.8–7.7)] was considerably (p = 0.000) lower than the N stage I–II groups [11.2 months (8.8–13.4)]. The predicted life expectancy for the group with pancreatic metastasis [4.1 months (2.6–5.5)] was significantly (p = 0.001) shorter than that of the group without pancreatic metastasis [8.9 months (7.6–10.1)]. The predicted life expectancy for the group with tumor size > 7 cm [6.7 months (5.4–8.0)] was significantly shorter than that of the group with tumor size of 0–3 cm [10.9 months (7.3–14.6)] (p = 0.019) and 3–7 cm [9.2 months (7.5–11)] (p = 0.023).

Conclusion
The authors of this study found that pancreatic metastasis, single metastasis, tumor diameter, and tumor N stage can be used as independent predictive factors for the survival of SCLC patients.