bluuebunny/crossref_metadata_2025_split huggingface dataset:
Further variation of the adulticide protocol for the treatment of canine heartworm infection: Can it be even shorter and cost less?
Authors: Ward Karen Ann , Jacobson Linda Susan , Lacaden Aveline Baldasan , Harrison Kelly Ann 
Published: December 2022

Abstract
Background

This retrospective study evaluated modified 3-dose melarsomine treatment protocols in a shelter setting and compared them to the American Heartworm Society (AHS) recommended protocol.

Methods

As compared to the AHS protocol, the shelter protocols utilized doxycycline 10 mg/kg once daily (SID) or twice daily (BID), and varied the time from initiation of doxycycline (day 1) to the first melarsomine injection (M1). Dogs were retrospectively grouped based on the shelter’s current protocol (M1 on day 14; Group A) and the AHS protocol (M1 on day 60; Group C), allowing a week on either side of the target M1 day. Treatments that fell outside of these ranges formed two additional treatment groups (Groups B and D). Respiratory complications were defined as respiratory signs requiring additional treatment, and were statistically compared for Groups A and C. New respiratory signs and gastrointestinal (GI) signs were compared between dogs receiving SID or BID doxycycline.

Results

One hundred fifty-seven dogs with asymptomatic or mild heartworm disease at presentation were included. All dogs survived to discharge. There was no statistically significant difference between Groups A (n = 79) and C (n = 27) for new respiratory signs post-melarsomine (P = 0.73). The time to M1 for 14 dogs that developed new respiratory signs was median 19 days, compared with 22 days for 143 dogs without new respiratory signs (P = 0.2). Respiratory complications post-melarsomine were uncommon. New respiratory signs post-melarsomine occurred in 10/109 (9.2%) dogs receiving SID doxycycline and 4/48 (8.3%) dogs receiving BID doxycycline (P > 0.999). GI signs prior to M1 were recorded for 40/109 (36.7%) dogs receiving SID doxycycline and 25/48 (52.1%) receiving BID doxycycline (P = 0.08). Forty-four follow-up antigen test results were available; all tests performed > 3 months after the third melarsomine injection were negative.

Conclusions

This study provided support for initiating melarsomine after 14 days of doxycycline and for a lower doxycycline dose. Shorter and less expensive treatment protocols can increase lifesaving capacity and improve quality of life for shelter dogs by reducing the duration of exercise restriction and length of stay.
Comprehensive benefits evaluation of highway sand control engineering based on matter-element extension model with variable weight
Authors: LI Liangying , LV Lele , YIN Wenhua , TAO Zhizhong , LI Qi 
Published: December 2022

Abstract
A scientific and reasonable sand control engineering can effectively reduce the impact of wind and sand activities on highway. The accurate evaluation of the comprehensive benefits about sand control engineering is the key to judging whether they can achieve the expected protective effect and ensure the smooth operation of desert highway. Through literature surveys and integrated analysis of typical highway sand control engineering in desert areas, a comprehensive benefits evaluation indicator system for highway sand control engineering was constructed from three dimensions: engineering benefits, economic benefits and environmental benefits. Then, the matter-element extension model with variable weight was introduced to calculate the indicator weights and a comprehensive benefit evaluation model for sand control engineering was proposed. The model allows the problem of distortion in evaluation result due to fluctuations in indicator values to be resolved. Combined with sand control engineering in the desert section of the Wuhai-Maqin Highway in China, the verification of the evaluation results was implemented. The research results show that the comprehensive benefits of the sand control engineering in the desert section of the Wuhai-Maqin Highway is in the “good” level, but it is slightly inclined towards the trend of the “medium” level. In this case, the ability to resisting wind and sand, construction quality and growth and restoration of vegetation, the three evaluation indicators of 13 evaluation indicators are the key indicators which make a significant impact on the comprehensive benefits. In the operation and maintenance stage, the growth and recovery ability of vegetation can be enhanced by strengthening the cultivation of vegetation, and the linkage of engineering, economy and environmental benefits can be promoted. The research results can be used as a reference for the evaluation of highway sand control engineering in wind and sand areas.
The Complete Chloroplast Genome of Endangered Species Dendrobium flexicaule: Insight into the Phylogenetic Relationship and Conservation Implications
Authors: Zhang Jie , Wang Le , Ming Xingjia , Shu Shu , Qu Xianyou , Zhang Jiayi , Liu Xue 
Published: December 2022

Abstract
Dendrobium flexicaule is an endangered species, listed at first-class National Key protected Wild Plant in China, narrowly endemic to China. It has a unique application different from other Dendrobium plants as a cure of infantile convulsions. Studying endangered species is helpful for current management and conservation.To compare analyze of the chloroplast genome (cp DNA) of D. flexicaule and other three related species(D. officinale, D. huoshanense, and D. moniliforme) and to analyze their chloroplast genome difference, we analyzed it from basic feature of chloroplast genome of D. flexicaule, codon usage and IR boundary, simple repeats, and phylogenetic tree, respectively. Download the chloroplast genome sequences of D. flexicaule, D. officinale, D. Huoshanense and D. moniliforme from the public database of NCBI were used to re-annotate by CPGAVAS2 software. We also constructed the MaximumLikehood phylogenetic tree using the MEGA-X tool.Comparing the IR/SC boundaries of the four Dendrobium species found that the IRb/SSC nodes of D. flexicaule and D.moniliforme expanded into the ndhF gene, while the IRb/SSC nodes of D. officinale and D. Huoshanense were between ycf1 and ndhF. The results of phylogenetic analysis show that D. flexicaule and D. officinaleare clustered together. The analysis of the cp genome of D. flexicaule provided crucial genetic information for further studies of this vulnerable species and the taxonomy, phylogenetics and evolution of Dendrobium.
System Dynamics Modeling to Compensate Co 2 Emissions in Road and Road-rail Transport Models in the Atlantic Forest Biome
Authors: Oliveira George , Silva Liniker Fernandes , Reis Leonardo Pequeno , Freitas Luís Carlos , Carvalho Jonatas Moreira Cruz 
Published: December 2022

Abstract

The objective of this study was to determine between the purchase of carbon credits and the activity of forest restoration in the Atlantic Forest which investment in CO2 emissions compensation is more attractive from an economic point of view and also to determine between the road and intermodal modes (road + rail) the most attractive option from an environmental point of view. To this end, a System Dynamics model was developed capable of converting CO2 emissions in restored forest areas in the Atlantic Forest biome and/or carbon credits from REDD + projects and estimating the costs associated with these offsets. The model was fed with data related to cargo transport in two modes: road and rail. For each modal, four different scenarios were developed for the offsetting of emissions: only forest plantation; only purchase of carbon credits; 50% forestry planting and 50% purchase of carbon credits; and 30% forestry planting and 30% purchase of carbon credits. The results indicate that the most attractive option from an economic point of view is the compensation of emissions through forestry planting and that the most attractive option from an environmental point of view is the use of the road-rail modal.
Existence of random mild solutions of a random integrodifferential equation with lack of compactness
Authors: El-Ghabi Adil , Khchine Abdelmjid , Taoudi Mohamed Aziz 
Published: December 2022

Abstract
In this paper, we show the existence of random mild solutions for a broad class of random integrodifferential equations with lack of compactness. The results are obtained using noncompact resolvent operators and a fixed point theorem of Monch type. Our results are applied to a large variety of partial differential equations in which memory effects are considered. Some examples are provided to illustrate the theoretical results.
Mathematics Subject Classification (2010). Primary 45K05, 47G20, 47D06, 47H10; Secondary 47H08.
Blockade of Erythropoietin Enhances the Abscopal Effects of Radiotherapy Restraining Lung Metastasis by Inducing an Immunopermissive Tumor Microenvironment
Authors: Liu Shin-Yi , Kuo Feng-Chi , Liou Wan-Zu , Chou Ying-Hsiang , Wu I-Fang , Lee Chi-Pin , Aicher Alexandra , Chen Yu-Jen 
Published: December 2022

Abstract

BackgroundDistant out-of-field, so-called abscopal, anti-metastatic effects of local radiation are rarely observed in cancer patients treated with radiotherapy alone. However, the era of immune checkpoint inhibitors (ICI) has increased abscopal effects following combinational treatment using radiotherapy and ICI (Radio-ICI). Hence, ICI-induced activation of cytotoxic T cells in the metastatic tumor microenvironment (TME) was instrumental in mediating the abscopal effect. Here, we hypothesized to improve the efficacy of abscopal effects observed in Radio-ICI through additional inhibition of immunosuppressive cells originating from the bone marrow. Therefore, we employed focal radiotherapy on the bone marrow of a single limb combined with ICI as alternative treatment for the induction of anti-metastatic abscopal responses.MethodsWe established lung metastatic mouse models by intravenous injection of colorectal cancer and melanoma cells, followed by single limb irradiation (SLI) treatment with 5 Gy to trigger abscopal effects. Tumor control, adverse effects, and composition of immune cells in the TME were monitored after radiotherapy as monotherapy or combinational therapy with ICI. Suppression of erythropoietin (EPO) using a neutralizing antibody was combined with SLI treatment to dissect the contribution of EPO signaling for the induction of abscopal effects. Functional markers for lymphoid and myeloid lineage cells, including subsets of immunosuppressive myeloid-derived suppressor cells (MDSC) and erythroid progenitor cells (EPC), were determined by flow cytometry, western blotting, and real-time PCR.ResultsSLI treatment alone induced a significant abscopal effect against lung metastases and enhanced the therapeutic efficacy of anti-PD-1. MDSC and EPC were suppressed after SLI exposure, accompanied by the reduction of M-CSF and EPO in the plasma of lung metastatic mice. Addition of EPO protein neutralized the SLI-induced antitumor response, while treatment with EPO antibody alone or in combination with SLI effectively inhibited tumor growth. Suppression of arginase 1 protein with concomitant increase of CD8 mRNA expression in the TME was observed after SLI treatment combined with EPO antibody. These effects were abrogated when SLI was combined with EPO protein.ConclusionSLI treatment induced an abscopal anti-metastatic tumor effect mitigating immunosuppressive barriers provided by MDSC and EPC, thus reversing the tumor-induced T cell dysfunction in the TME.
D3 lymph node dissection improves the survival outcome in patients with pT2 colorectal cancer
Authors: Wang Liming , Song Bolun , Chen Yinggang , Hirano Yasumitsu 
Published: December 2022

Abstract
Background: The extent of lymphadenectomy in patients with pT2 colorectal cancer (CRC) remains controversial. This study aimed to elucidate the effects of D3 and D2 lymph node dissection (LND) on survival in patients diagnosed with pT2 CRC.
Methods: This was a retrospective cohort study from a high-volume cancer center in Japan. From April 2007 to December 2020, 6,273 patients with primary CRC were included in the study; among these, 616 patients diagnosed with pT2 CRC underwent radical colorectal resection. Propensity score matching (PSM) was applied to balance potential confounding factors and a total of 104 matched pairs were extracted from the entire cohort. Independent risk factors associated with prognosis were determined by Cox regression analysis. The main outcome measures were overall survival (OS) and cancer-specific survival (CSS).
Results: Before PSM, there was a statistically significant difference across the cohort in OS and CSS (p=0.000 and 0.013) between D3 and D2 LND groups; the estimated hazard ratio (HR) was 2.2 (95% confidence interval (CI), 1.1-4.4, p=0.031) for OS in the D3 LND and 4.4 (95% CI, 1.7 to 11, p=0.0027) for CSS (p=0.013). There was also a significant difference (p=0.024) in OS between the D3 and D2 LND groups in the matched cohort, with an estimated HR for OS of 3.3 (95% CI, 1.2 to 9.1, p=0.024) and an estimated HR for CSS of 7.2 (95% CI, 1.6 to 33, p=0.011).
Conclusions:
D3 LND had a significant survival advantage in the treatment of pT2 colorectal cancer. The results of this study provide a theoretical basis for the application of D3 LND in radical surgery for pT2 colorectal cancer.
Copper-inducible expression system for metabolic engineering of Escherichia coli
Authors: Liu Lingrui , Zhang Lu , Wang Junyi , Cao Mingfeng , Yuan Jifeng 
Published: December 2022

Abstract
Background
The inducible expression system plays an important role in engineering Escherichia coli for chemical production, however, it still heavily relies on expensive chemical inducers, like IPTG. There is a pressing need to develop alternative expression systems with more affordable inducers.
Results
We herein reported a copper-induced gradient expression system in E. coli based on the two-component Cus system and T7 RNA polymerase (RNAP). By integrating the gene encoding T7 RNAP at the CusC locus, we managed to program eGFP expression under T7 promoter in response to different concentrations of Cu2+ (0–20 µM). Subsequently, we demonstrated that the copper-induced gradient expression system was suitable for metabolic engineering of E. coli towards protocatechuic acid (PCA) overproduction, and the resulting strain with combined manipulation of the central metabolism via CRISPRi produced 4.12 g/L PCA under the optimal copper concentration and induction time.
Conclusions
We established a copper-inducible T7 RNAP expression system in E. coli. The copper-induced gradient expression system could rationally control metabolic pathways in a temporal and dose-dependent manner. The gradient expression system based on copper inducer could be widely used in E. coli cell factories, and the design principle reported here would also be applicable in other prokaryotes.
Socio-Economic Impacts of FIFA World Cup Qatar 2022: A Study in Chon Buri Sports City Residents
Authors: Chankuna Dittachai 
Published: December 2022

Abstract
Hosting FIFA World Cup obviously produce immense socio-economic impacts to a nation. Proximal non-host city perception to mega event was frequently explored, while distal non-host city perception has less investigated. This study assessed the socio-economic impacts of FIFA World Cup Qatar 2022 in Chon Buri Sports City residents. Participants were 422 Chon Buri Sports City residents from eleven districts. Perception on socio-economic impacts of hosting FWC Qatar 2022 was measured from the translated Scale of Perceived Social Impacts. Extracted seven dimensions by confirmatory factor analysis is consistent with empirical data. Positive impact dimensions are Community Development, Community Pride, Economic Benefits. Negative impact dimensions are Traffic Problems, Security Risks, Economic Costs, and Environmental Concerns. Chon Buri Sports City residents perceived fewer negative impacts than other non-host city residents. Positive impacts of hosting FWC Qatar 2022 has more influenced on Chon Buri Sports City residents’ perception than negative impacts. These findings advantage for sport stakeholders to deeper understand the impacts of mega event hosting.
One Pattern Analysis (OPA) for the quantitative determination of protein interactions in plant cells
Authors: Maika Jan Eric , Strotmann Vivien Ingeborg , Krämer Benedikt , Wellmer Frank , Weidtkamp-Peters Stefanie , Stahl Yvonne , Simon Rüdiger 
Published: December 2022

Abstract
Background:

A commonly used approach to study the interaction of two proteins of interest (POIs) in vivo is measuring Förster Resonance Energy Transfer (FRET). This requires the expression of the two POIs fused to two fluorescent proteins that function as a FRET pair. A precise way to record FRET is Fluorescence Lifetime IMaging (FLIM) which generates quantitative data that, in principle, can be used to resolve both complex structure and protein affinities. However, this potential resolution is often lost in many experimental approaches. Here we introduce a novel tool for FLIM data analysis of multiexponential decaying donor fluorophores, One Pattern Analysis (OPA), which allows to obtain information about protein affinity and complex arrangement by extracting the relative amplitude of the FRET component and the FRET transfer efficiency from other FRET parameters.

Results:

As a proof of concept for OPA, we used FLIM-FRET, or FLIM-FRET in combination with BiFC to reassess the dimerization and tetramerization properties of known interacting MADS-domain transcription factors in Nicotiana benthamiana leaf cells and Arabidopsis thaliana flowers. Using the OPA tool and by extracting protein BINDING efficiencies from FRET parameters to dissect MADS-domain protein interactions in vivo in transient N. benthamiana experiments, we could show that MADS-domain proteins display similar proximities within dimeric or tetrameric complexes but bind with variable affinities. By combining FLIM with BiFC, we were able to identify SEPALLATA3 as a mediator for tetramerization between the other MADS-domain factors. OPA also revealed that in vivo expression from native promoters at low levels in Arabidopsis flower meristems, in situ complex formation of MADS-domain proteins cannot be detected.

Conclusions

We conclude that MADS-domain protein interactions are transient in situ and may involve additional, so far unknown interaction mediators. We conclude that OPA can be used to separate protein binding from information about proximity and orientation of the interacting proteins in their complexes. Visualization of individual protein interactions within the underlying interaction networks in the native environment is still restrained if expression levels are low and will require continuous improvements in fluorophore labelling, instrumentation set-ups and analysis tools.
Unveiling the gemcitabine drug complexation with cucurbit[n]urils (n=6-8): A computational analysis
Authors: Venkataramanan Natarajan Sathiyamoorthy , Suvitha Ambigapathy , Sahara Ryoji , Kawazoe Yoshiyuki 
Published: December 2022

Abstract
In this work, the DFT-D3 method was employed to investigate the complex formation capability of gemcitabine drug with host cucurbit[n]uril Q[n] (n = 6,7 and8) molecules. The density functional theory studies demonstrate that the most stable configuration is a fully encapsulated complex. In the gemcitabine@[6] and gemcitabine@[7] encapsulated systems the gemcitabine amino -NH2 and the alcoholic group in the carbohydrate bonds with the carbonyl units of Q[n]. The addition of sodium ions leads to the partial exclusion of the gemcitabine molecule and the sodium atoms lie close to the carbonyl portal of Q[7]. Thermodynamic parameters computed for the complexation process exhibit high negative entropy change implying that the encapsulation process is spontaneous and is an enthalpy-driven process. Frontier molecular orbitals are located mainly on the gemcitabine uracil ring, before and after encapsulation formation, indicating that the encapsulation happens by pure physical adsorption. Quantitative molecular electrostatic potentials demonstrate a shift in charge occurs during the complex formation and is more pronounced in gemcitabine@Q[7]. AIM topological analysis illustrates that these complexes are stabilized by various noncovalent interactions including HBs and C···F interactions. The 2D RDG plots exhibit the presence of strong HBs and weak van der Waals interactions and the presence of steric repulsion. The isosurface NCI diagram shows predominant steric interaction in the gemcitabine@Q[6] complex. The NCI isosurface for gemcitabine encapsulated complexes with Q[7] and Q[8] host displays that the green patches are uniformly distributed in all directions. Finally, EDA results demonstrate Paulis repulsive energy is predominant in the gemcitabine@Q[6] complex, while the orbital and dispersion energies stabilize the gemcitabine@Q[7] complex.
Exploring the dynamics of triglyceride with stroke onset in the Chinese population through parameterized joint models
Authors: Mao Qian , Gao Wenfeng , Yang Liping , Yang Yi , Xu Yaqi , Cong Huiwen , Wang Lianyuan , Shi Fuyan , Wang Suzhen 
Published: December 2022

Abstract
Background: Stroke has become one of the diseases with the highest mortality and disability rates in the world, especially in low-income and developing countries. Our objective was to discuss the relationship between the longitudinal dynamic changes of triglyceride and stroke onset in healthy populations by constructing different parametric joint models.
Methods: 298 participants aged 23 to 69 in Xijing hospital of Xi’an City in Shanxi Province from 2008 to 2015 were included. The Cox proportional hazards model was performed to analyze the correlation between triglyceride and stroke incidence at baseline. Different parameterized joint models were used to analyze the impact of dynamic changes of triglyceride on the incidence of stroke under longitudinal data.
Results: Of the 298 participants, a total of 70 (23.49%) subjects developed stroke during the study period. Cox proportional hazards model showed that the risk of disease increased by 1.056 times (95%CI=0.920-0.975) for each 1 unit of baseline age decrease. Each 1 mmol/L increase in sqrt(triglyceride) increased the risk by 1.816 times (95%CI=1.017-3.245). Joint model showed that the risk of sqrt(triglyceride) increased by 4.869 times (95%CI=3.987-8.857) for each 1 mmol/L increase in the longitudinal direction. The lagged effects (HR=5.284, 95%CI=4.397-9.680) and cumulative effects (HR=1.786, 95%CI=1.613-3.399) of sqrt(triglyceride) dynamic trajectory were also statistically related to the incidence of stroke.
Conclusions: Over time, the longitudinal growth of triglyceride levels in individuals will increase the risk of stroke even more. People should pay more attention to the dynamic changes of individual triglyceride values, as well as the lagged effect and cumulative effect, to reduce the incidence of stroke.
Metody obrazowania w radioterapii sterowanej obrazem
Authors: Graczyk Kinga , Kruszyna-Mochalska Marta 
Published: December 2022

Radioterapia sterowana obrazem (ang. Image-Guided Radiation Therapy – IGRT) to technika wykorzystująca obrazowanie dwu- i trójwymiarowe. Stosowana jest, aby dokładnie zlokalizować objętość leczoną przed ekspozycją pacjenta na wiązkę terapeutyczną, generowaną w celu realizacji planów leczenia zapewniających wysoką precyzję dostarczania dawki, opartych na technikach z modulacją intensywności wiązki.
Celem niniejszej pracy było omówienie używanych obecnie w radioterapii sterowanej obrazem metod obrazowania - wolumetrycznych i planarnych, opartych na wykorzystaniu promieniowania jonizującego i pozostałych, które opierają się na zjawiskach niejonizacyjnych.