bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does higher knee hyperextension in patients with hemiplegia affect lateral and medial meniscus volume in the paretic leg? A Cross-sectional study
Authors: Li Wenshan , Li Tiancong , Xi Xiaoshuang , Zhang Rong , Sun Weishuang , Zhang Dan , Gong Weijun 
Published: August 2022

Abstract
Background: After stroke, an abnormal gait pattern gradually leads to knee pain and joint lesions, resulting the gait instability. However, the correlation between the knee hyperextension and gait pattern, the meniscus volume, and the water content of meniscus in paretic and non-paretic legs has not been fully investigated. Moreover, most of physicians tend to ignore this knee hyperextension. This study attempted to emphasize the importance of knee hyperextension using gait analysis and Magnetic resonance imaging (Trial registration number ChiCTR2000039641, date of registration 04/11/2020).Methods: Eight patients with chronic hemiplegic (6 male, 2 female) volunteered to participate in this study. Participants was recruited if they had a hemiplegia following a stroke occurring more than 6 months, had an ability to walk 10 meters without aids, had a Function Ambulation Category level at least 3 and above, and had a hemiplegic lower extremity identified as Brunnstrom state III or above identification. The spatial-temporal gait parameters and kinematic parameters in the paretic and the non-paretic legs and the percentage of free water content in deep and shallow layers.Results: Longer time since hemiplegia led to larger angles of knee hyperextension (R = 0.56, p = 0.016), larger angles of knee hyperextension led to more tears in meniscus (R = - 0.53, -0,57 and -0.70), and larger angles of knee hyperextension decreased water content of the lateral meniscus in the non-paretic leg (R = -0.91) but increased water content of the medial meniscus (R = 0.53 and 0.63).Conclusions: The knee hyperextension could not be ignored by physicians and needed to be diagnosed and treated as early as possible, the time since hemiplegia could be an indicator of sign of knee hyperextension. https: //doi.org/10.1101/2022.02.18.22271154
Hepatocyte growth factor-mediated apoptosis mechanisms of cytotoxic CD8+ T cells in normal and cirrhotic liver
Authors: Bai Lianhua , Chen Quanyu , Yan Min , Lin Heng , Lai Jiejuan , Yang Zhiqing , Hu Deyu , Deng Yuanyu , Shi Saiyu , Shuai Ling , Zhang Leida , Zhang Hongyu 
Published: August 2022

Abstract
Intrahepatic stem/progenitor cells and cytotoxic CD8+ T cells (CD8+ T cells) in the cirrhotic liver undergo apoptosis potentially facilitating progression to cancer. Here, we report that hepatocyte growth factor (HGF) signaling plays an important role in promoting normal and damaged liver CD8+ T cell Fas-mediated apoptotosis through its only receptor c-Met. In addition to binding with HGF, c-Met also binds to Fas as a complex. Using a diethylnitrosamine (DEN)-induced liver fibrosis/cirrhosis mouse model, immunofluostaining and terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) staining, we identified significantly increased HGF secretion at 10 weeks post-DEN, the liver cirrhotic phase (LCP), compared to it at 3 weeks post-DEN, the liver fibrotic phase (LFP). Correspondingly, difference CD8+ T cell proliferation and apoptosis were noted in the two phases, Interestingly, staining and TUNEL identified that higher smooth muscle actin (α-SMA)+ cell apoptosis, a marker for hepatic stellate cell (HSC) in the LFP compared to their in the LCP, suggesting that beneficial correlation of HGF, CD8+ T cells and HSC in improving fibrotic load during damaged liver repair. In cultures, up to 200 ng/mL amounts of recombinant HGF the naive mouse splenic CD8+ T cells (n-msCD8+ T cells) death, 400 ng/mL rHGF show directly activated death-inducing signaling complex (DISC) to recruit FADD and caspase-8 in both nsCD8+ T cells and healthy human peripheral blood CD8+ T cells (hp-CD8+ T cells), suggesting Fas-mediated apoptosis, may suggest the regulating role of HGF signaling in hepatic homeostasis.
Comparative transcriptome analysis reveals the biological processes of hybridization incompatibility between Brassica napus and B. oleracea
Authors: Yue Fang , Zheng Fajing , Li Qinfei , Qian Wei 
Published: August 2022

Abstract
It is an important breeding strategy to improve Brassica napus by crossing with its parental species, B. oleracea and B. rapa. Interspecific hybridization between B. napus and B. rapa is compatible with high rate of survival embryo, while the hybridization between B. napus and B. oleracea is incompatible with high occurrence of embryo abortion. To understand the diverse embryo fate in the two interspecific hybridizations, here the siliques of B. napus pollinated with B. oleracea (AE) and B. rapa (NE) were employed for transcriptome sequencing at 8 and 16 days after pollination. Compared with NE and female, there were 1274 and 1698 differentially expressed genes (DEGs) in AE at 8 and 16 days after pollination (AE8 and AE16), which were significantly enriched into 67 and 72 GO terms, respectively. Of which 9 and 15 GO terms in cell growth, cell wall formation harbored high ratio of down-regulated expression DEGs (96/131 in AE8, 174/235 in AE16), while 12 and 17 GO terms in the processes of photosynthesis, photorespiration, peroxisome, oxidative stress and systemic acquired resistance harbored high ratio of up-regulated expression DEGs (222/304 in AE8, 214/287 in AE16). A high level of O2−, H2O2 content and antioxidation activity (SOD, CAT) was tested in the siliques of B. napus pollinated with B. oleracea. Our data suggest that disorder of plant hormone metabolism, retardation of cell morphogenesis and the accumulation of reactive oxygen species (ROS) may associate with hybridization incompatibility between B. napus and B. oleracea.
The transcription factor HBP1 activates ferroptosis in tumor cells by regulating the UHRF1-CDO1 axis
Authors: Zhang Xiaowei , Yang Ruixiang , Zhou Yue , Zhang Tongjia , Wang Shujie , Wang Jiyin , Cheng Yuning , Li Hui , Jiang Wei , Zhe Yang 
Published: August 2022

Abstract
The induction of ferroptosis in tumor cells is one of the most important mechanisms by which tumor progression can be inhibited; however, the specific regulatory mechanisms underlying ferroptosis remain unclear. In this study, we investigated the important role of HBP1 in ferroptosis. HBP1 down-regulates the protein levels of UHRF1 by inhibiting the expression of the UHRF1 gene at the transcriptional level. Reduced levels of UHRF1 have been shown to regulate the ferroptosis-related gene CDO1 by epigenetic mechanisms, thus up-regulating the level of CDO1 and promoting ferroptosis in tumor cells. On this basis, we constructed metal-polyphenol-network coated HBP1 nanoparticles by combining biological and nanotechnological. MPN-HBP1 nanoparticles entered tumor cells efficiently and innocuously, induced ferroptosis and inhibited the malignant proliferation of tumors by regulating the HBP1-UHRF1-CDO1 signaling pathway. This study provides a new perspective for further research on the regulatory mechanism underlying ferroptosis and its potential role in tumor therapy.
Tumor Lesion Detection in Patients with Cervical Cancer by Indocyanine Green Near-Infrared Imaging
Authors: Li Pengfei , Liu Jiaqi , He Kunshan , Gong Shipeng , Chi Chongwei , Liu Pan , Su Guidong , Li Weili , Duan Hui , Liu Ping , Tian Jie , Chen Chunlin 
Published: August 2022

AbstractPurpose：To investigate the feasibility and accuracy of near-infrared fluorescence (NIRF) imaging for detecting the extent of tumor invasion in cervical cancer using indocyanine green (ICG).Methods:We enrolled 51 patients who were diagnosed with cervical cancer with FIGO stage IB1-IIA2 disease. Patients were administered indocyanine green (ICG) at a dose of 5 mg/kg 24 hours prior to surgery. A customized near-infrared fluorescence (NIRF) imaging system was used to identify the extension of tumor invasion when radical hysterectomy specimens were harvested. The relationship between tumor fluorescence intensity and clinicopathological characteristics was analyzed.Results:Three patients were without residual tumors after cervical conization, and tumor lesions were identified by NIRF imaging in all the remaining 48 patients. The results of NIRF imaging were in agreement with the postoperative pathological findings in 95.8% of the patients with stromal invasion, 100% of those with surgical margin invasion, 100% of those with parametrial tumor involvement, and 100% of patients with uterine corpus invasion. The mean signal-to-background ratio (SBR) of the cervical tumors was 2.91±1.64, and the SBR was independent of clinicopathological characteristics. Fluorescence microscopy confirmed that ICG fluorescence was present in the tumor nests.Conclusions:NIRF imaging enables objective, accurate and safe identification of tumor invasion during cervical cancer surgery.Clinical Trial Registration:ClinicalTrials.gov NCT04224467.
An MMSPEED based droplet Routing protocol for Digital Microfluidic Biochip
Authors: Swain Jyotiranjan , Pyne Sumanta 
Published: August 2022

Abstract

Droplet routing is very important phase in biochemical synthesis using digital microfluidic biochip. The goal is to transport droplet from one module to other maintaining fluidic constraint at every instant of time. In this paper we proposed a new droplet routing based on MMSPED routing protocol. It use multiple copies of route request packets to explore and discover the routes. To validate these routes, hello packets are used.The routes are then classified and sorted based on a user defined heuristics. Finally, route compaction is performed to generate the parallel moving sequence. Simulation result shows 12.25% and 20.5% improvement in latest arrival time for free and virtual topology respectively.
Modeling a Remanufacturing Reverse Logistics Planning Problem: Some Insights into Disruptive Technology Adoption
Authors: Yu Hao 
Published: August 2022

AbstractRemanufacturing is the process to restore the functionality of high-value Endof-life (EOL) products, which is a substantial link in reverse logistics systems for value recovery. However, due to the uncertainty of the reverse material flow, the planning of a remanufacturing reverse logistics system is complex. Furthermore, the increasing adoption of disruptive technologies in Industry 4.0/5.0, e.g., Internet of things (IoT), smart robots, cloud-based digital twin, additive manufacturing, etc., have shown a great potential for a smart paradigm transition of remanufacturing reverse logistics operations. In this paper, a new mixed-integer program is modeled for supporting several tactical decisions in remanufacturing reverse logistics, i.e., remanufacturing setups, production and inventory levels, purchase and transportation, and remanufacturing line utilization and balancing. The model is further extended by incorporating utilization-dependent nonlinear idle time cost constraints and stochastic takt time to accommodate different real-world scenarios. Through a set of numerical experiments, the influences of different demand patterns and idle time constraints are revealed. The potential impacts of disruptive technology adoption in remanufacturing reverse logistics are also discussed from managerial perspectives, which may help remanufacturing companies with a smart and smooth transition in the Industry 4.0/5.0 era.
Lymphedema therapy referral is associated with improved understanding of lymphedema prevention among breast cancer survivors
Authors: Klugman Madelyn , Tringale Kathryn R. , Patil Sujata , Montagna Giacomo , Finik Jackie , Kuo Ting-Ting , Eberle Carolyn , Pinkhasov Alexandr , Gillis Theresa , Gany Francesca , Blinder Victoria 
Published: August 2022

Abstract
Purpose: Lymphedema is common among breast cancer survivors (BCS) and negatively affects quality of life. There are modifiable factors to reduce lymphedema risk and exacerbation; it is unknown how aware survivors are of these factors. We hypothesized referral to lymphedema therapy (LT) to be associated with greater lymphedema knowledge.Methods: BCS at an NCI-designated cancer center between 2014-2015 were asked to complete an anonymous questionnaire. Eligibility criteria were the following: age ≥18, female sex, English-speaking, > 6 months post definitive breast cancer surgery, no cancer recurrence, and no prior or subsequent second cancer. The questionnaire included sociodemographics, clinical factors including LT referral, and 10 true/false questions assessing lymphedema knowledge. Multivariable logistic regressions assessed the relationship between prior LT referral and correctly answering questions about lymphedema misconceptions.Results: Of 209 participants, 53 (25%) had been referred to LT. Those who had sentinel lymph node dissection were less frequently referred to LT [15 (14%)] than those who had axillary lymph node dissection [38 (39%)]. Five of the true/false questions had a correct response rate of <80%. In multivariable regression adjusted for age, race/ethnicity, education, axillary surgery, and radiation therapy, LT referral was associated with answering the questions about weight gain and exercising the arm on an airplane correctly [weight gain: odds ratio, 95% confidence interval (OR, 95%CI): 3.63 (1.66-7.96), airplane: 2.65 (1.15-6.13)].Conclusion: Misconceptions surrounding lymphedema prevention and management are common among BCS. LT referral is a potential opportunity to debunk common misunderstandings regarding lymphedema risk reduction.
Investigating the Effect of Zinc Supplementation on Probability of Relapse and Mental Health in Patients with Substance Abuse Disorder Undergoing Methadone Maintenance Treatment
Authors: Amini Zahra , HeidariFarsani Ebrahim 
Published: August 2022

Abstract
Background Considering different factors such as high withdrawal rates in methadone maintenance treatment (MMT) programs alongside mental health (MH) problems appearing in patients with substance abuse disorder and the lack of prior research on the effect of zinc supplementation in this respect, the present study aimed to investigate the effect of zinc supplementation on probability of relapse (PoR) and MH problems in patients with substance abuse disorder undergoing MMT.Methods For this purpose, a randomized controlled trial with a clinical basis was fulfilled on a total of 68 patients with substance abuse disorder receiving MMT, allocated to two groups, viz. intervention and control (each one consisting of 34 individuals). Then, the participants in the intervention group were given zinc supplements combined with methadone for three months and the controls only took methadone, according to the treatment plan. The data were collected using the Relapse Prediction Scale (RPS) and the Depression, Anxiety and Stress Scale 21 (DASS-21) before, one month after, and at the end of the intervention program.Findings Compared to the control group, PoR (p=0.01), drug craving (p=0.002), and the RPS total score (p=0.002) in the intervention group were significantly lower. Moreover, the results revealed a significant decreasing trend in depression (p=0.01), anxiety (p<0.001), stress (p = 0.001), and the DASS-21 total score (p=0.001) in the intervention. Compared to the control group, the DASS-21 total score (p<0.001) in the intervention group were significantly lower.Conclusion Accordingly, it was concluded that zinc supplementation could reduce the PoR and improve MH problems in patients with substance abuse disorder experiencing MMT. However, further research is recommended to fill the gaps.Trial Registration The research protocol has been also listed on the Iranian Registry of Clinical Trials (IRCT) with the code no. IRCT2020050904736N1
Highly Sensitive and Selective Surface Molecularly Imprinted Polymer Electrochemical Sensors Prepared by Au and MXene Modified Glassy Carbon Electrode for Efficient Detection of Tetrabromobisphenol A in Water
Authors: Shao Yanming , Zhu Ying , Zheng Rui , Wang Peng , Zhao Zhizhen , An Jun 
Published: August 2022

Abstract
Tetrabromobisphenol A (TBBPA) was a widely used brominated flame retardants (BFRs) to impede or inhibit flammability. It was detected in aquatic food webs, soils, and sediments from diverse electronic waste (e-waste) recycling sites. A selective and sensitive electrochemical sensor which was construct by dropped molecular imprinted polymer onto MXene and Au nanoparticles modified glassy carbon electrode was used to detect TBBPA. The specific recognition site provided by molecular imprinted polymer can ensure the accuracy of detection results. Excellent conductivity of MXene and Au nanoparticles can effectively enhance the responsive signal of the sensors. Fe3O4 nanoparticles was firstly fixed on graphene sheets via the solvothermal method, and the azido was introduced by nucleophilic addition between carbonyl and amino after dopamine was self-polymerized onto the surface of Fe3O4 nanoparticles. Molecularly imprinted polymer was prepared by RAFT polymerization after RAFT agent was introduced by the click chemistry between azido and alkynyl. The concentration of TBBPA was detected by differential pulse voltammetry. The influences of accumulation time and pH value were investigated. The as-prepared molecular imprinting sensor exhibited a good linear relationship to the concentration of TBBPA range from 0.05 to 10 nM with a detection limit of 0.0144 nM. The proposed sensor was also successfully used to detect TBBPA in water samples and the recovery for the standards added was 97.1–106%.
Erythrocyte membrane integrity and protein activity in high fat diet fed male Wistar rats
Authors: Ogbara Festus Freedom , Ige Abayomi O. , Adele Bernard Omokheshi , Adewoye Elsie Olufunke 
Published: August 2022

Abstract

PurposeHigh fat diet (HFD) has been reported to induce systemic inflammation and oxidative stress that may affect the structural integrity of erythrocytes and alter their ability to undergo deformation. This study was therefore designed to investigate the effect of HFD feeding on erythrocyte membrane integrity and protein activity in male Wistar rats.MethodsTen animals (100-120g) were grouped equally and exposed to either standard diet or HFD (25%-fat) for 42days. Thereafter, retro-orbital sinus blood was collected under anesthesia (thiopental) and aliquots were analyzed for erythrocyte sedimentation rate (ESR), osmotic fragility and mean corpuscular fragility. Erythrocyte ghost membranes were also isolated from blood sample aliquots and analyzed for total protein concentration, malondialdehyde (MDA), Na+K+-ATPase activity, Ca2+Mg2+-ATPase activity and intercellular adhesion molecule (ICAM)-4 level.ResultsOsmotic fragility and mean corpuscular fragility were significantly increased (P<0.05) in the HFD-fed group compared to control. ESR (mm/hr) (64.60±2.34 vs. 21.20±1.53), membrane MDA (µMol) (3.66±0.86 vs. 0.43±0.08) and ICAM-4 (ng/ml) (1.68±0.23 vs. 0.49±0.16) levels were also increased (P<0.05) in the group 2 (HFD) compared to group 1 (standard diet). Compared to standard diet group, erythrocyte membrane total protein concentration (10.46±0.96 vs 6.00±0.38 g/dl) and Na+K+ATPase activity (1.37±0.22 vs 0.22±0.03 x107µmol pi/mg protein/hr) was reduced (P<0.05) in the HFD group while Ca2+Mg2+-ATPase exhibited a 27.9% increase in activity.ConclusionThis study suggests that high fat diet may compromise the structural and functional integrity of erythrocytes by activating systemic inflammation, erythrocyte membrane and protein oxidation as well dysregulated membrane ATPase activity required to maintain erythrocyte deformability in male Wistar rat.
Chidamide induces EBV lytic infection and synergistically with tenofovir eliminate EBV-positive Burkitt lymphoma and inhibit EBV DNA replication
Authors: Xu Linyan , Zhang Meng , Tu Dongyun , Lu Ziyi , Lu Tianyi , Ma Dongshen , Zhou Yi , Zhang Shuo , Ma Yuhan , Yan Dongmei , Wang Xiangmin , Sang Wei 
Published: August 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
LMSuccSite: Improving Protein Succinylation Sites Prediction Using Embeddings from Protein Language Model
Authors: Pokharel Suresh , Pratyush Pawel , Heinzinger Michael , Newman Robert , KC Dukka 
Published: August 2022

Abstract
Protein succinylation is an important post-translational modification (PTM) responsible for many vital metabolic activities in cells, including cellular respiration, regulation, and repair. Here, we present a novel approach that combines features from supervised word embedding with embedding from a protein language model called ProtT5-XL-UniRef50 (hereafter termed, ProtT5) in a deep learning framework to predict protein succinylation sites. To our knowledge, this is one of the first attempts to employ embedding from a pre-trained protein language model to predict protein succinylation sites. The proposed model, dubbed LMSuccSite, achieves state-of-the-art results compared to existing methods, with performance scores of 0.36, 0.79, 0.79 for MCC, sensitivity, and specificity, respectively. LMSuccSite is likely to serve as a valuable resource for exploration of succinylation and its role in cellular physiology and disease.
Data-Driven Contagion Risk Management in Low-Income Countries: Machine Learning Applications with COVID-19 in South Asia
Authors: Shonchoy Abu , Mahzab Moogdho , Mahmood Towhid , Ali Manhal 
Published: August 2022

Abstract

Objectives To derive and validate a data-driven Contagion Risk Index (CR-Index) at sub-national level for low-income countries – detecting potential infection hotspots – aiding policymakers with efficient mitigation planning for COVID-19 and future epidemics and pandemics. Methods We utilize daily district-level COVID-19 data (positive cases and deaths) from South Asia (India, Pakistan, and Bangladesh) from 2020–2022 to derive the CR-Index – founded on commutable disease spreadability vectors across four domains: urbanization, informality, migration, and health infrastructure. We validated CR-Index based risk-zoning by utilizing time-series regressions and machine learning (ML) estimates (Random Forests and a battery of cross-validation) for predictive accuracy. Results Regressions demonstrate a strong association between the CR-Index and sub-national COVID-19 epidemiology data. ML driven validation show strong predictive support for the CR-Index that can distinguish districts with high-risk COVID-19 cases/deaths for more than 85% of the time. Conclusion Our proposed simple and replicable CR-Index is an easily interpretable tool that can help low-income countries to prioritize resource mobilization (such as vaccination roll-out or free in-home test-kits) to contain the disease spread and associated crisis management, with global relevance and applicability.