bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reported Neurologic, Ocular, and Otic Manifestations Among Syphilis Cases—16 States, 2019
Authors: Jackson David A. , McDonald Robert , Quilter Laura A.S. , Weinstock Hillard , Torrone Elizabeth A. 
Published: July 2022

Background
Syphilis can cause neurologic, ocular, or otic manifestations, possibly resulting in permanent disability or death. In 2018, the Centers for Disease Control and Prevention began collecting syphilis clinical manifestation data via the National Notifiable Diseases Surveillance System. We present the first reported US syphilis neurologic, ocular, and otic manifestation prevalence estimates.

Methods
We reviewed 2019 National Notifiable Diseases Surveillance System data to identify jurisdictions reporting 70% or greater of syphilis cases 15 years or older with clinical manifestation data (considered “complete reporting”). Among these jurisdictions, we determined reported neurologic, ocular, and otic manifestation prevalence, stratified by demographic, behavioral, and clinical characteristics.

Results
Among 41,187 syphilis cases in 16 jurisdictions with complete reporting, clinical manifestations were infrequently reported overall: neurologic (n = 445, 1.1%), ocular (n = 461, 1.1%), otic (n = 166, 0.4%), any (n = 807, 2.0%). Reported clinical manifestation prevalence was highest among cases 65 years or older (neurologic, 5.1%; ocular, 3.5%; otic, 1.2%) and those reporting injection drug use (neurologic: 2.8%; ocular: 3.4%; otic: 1.6%). Although reported neurologic and ocular manifestation prevalence was slightly higher among human immunodeficiency virus (HIV)-infected versus HIV-negative persons, approximately 40% of cases with manifestations were HIV-negative. Reported otic manifestation prevalence was similar regardless of HIV status. When stratifying by HIV status and syphilis stage, reported prevalence was highest among HIV-infected persons with unknown duration/late syphilis (neurologic, 3.0%; ocular, 2.3%; otic, 0.7%).

Conclusions
Reported neurologic, ocular, and otic manifestation prevalence was low among syphilis cases, but these data are likely an underestimate given potential underreporting. Reported clinical manifestation frequency, including among HIV-negative persons, emphasizes the importance of evaluating all syphilis cases for signs/symptoms of neurosyphilis, ocular syphilis, and otosyphilis.
An updated nomenclature for plant ribosomal protein genes
Authors: Scarpin M. Regina , Busche Michael , Martinez Ryan E. , Harper Lisa C. , Reiser Leonore , Lan Ting , Xiong Wei , Mo Beixin , Tang Guiliang , Chen Xuemei , Bailey-Serres Julia , Browning Karen , Brunkard Jacob O. 
Published: July 2022

Ban et al. (2014) proposed a nomenclature for ribosomal proteins (r-proteins) that reflects the current understanding of ribosomal protein evolution. In the past few years, this nomenclature has been widely adopted among biomedical researchers and microbiologists. This homology-based r-protein nomenclature has not been as widely adopted among plant biologists, however, presumably because r-protein nomenclature is much more complicated in plants due to gene duplication. Here, we propose compatible upgrades to the homology-guided nomenclature proposed by Ban et al. (2014) so that this naming system can be adopted for widespread use in the plant biology community. We note that Lan et al. (2022) recently proposed updated nomenclature for plant cytosolic ribosomal proteins, focused on Arabidopsis and rice. The nomenclature outlined here is an extension of that proposed by Lan et al. (2022), expanding to include organellar ribosomes and additional species, with the intent that this nomenclature can serve as a template to guide future plant genome annotations. A more detailed comparison highlighting how this naming system builds on the Ban et al. (2014) and Lan et al. (2022) nomenclatures is offered below.At this time, we request community feedback on this proposed nomenclature so that the naming system ultimately chosen represents a broad consensus. Feedback can be communicated to the this working group at plantribosome@gmail.com before July 25th, 2022. Coauthors of this letter and anyone in the scientific community expressing significant interest will then discuss this feedback as a group, reach a consensus agreement, and communicate the updated nomenclature rules through a letter to the editor (expected to be published at The Plant Cell) and the databases at TAIR and MaizeGDB.
Effects of age and sex on dendritic D2 autoreceptor inhibition in substantia nigra dopamine neurons
Authors: Troyano-Rodriguez Eva , Handa Kylie , Branch Sarah Y , Beckstead Michael J 
Published: July 2022

ABSTRACTSubstantia nigra pars compacta (SNc) dopamine neurons are required for voluntary movement and reward learning, and advanced age is associated with motor and cognitive decline. In the midbrain, D2-type autoreceptors located on dendrodendritic synapses between dopamine neurons control cell firing through G protein-activated potassium (GIRK) channels. We previously showed that aging disrupts dopamine neuron pacemaker firing in mice, but only in males. Here we show that D2-receptor inhibitory postsynaptic currents (D2-IPSCs) in aged male mice are moderately smaller compared to young males as well as females, regardless of age. Local application of dopamine revealed a reduction in the amplitude of the D2-receptor currents in old males compared to young, pointing to a postsynaptic mechanism that could not be explained by impairment of the GIRK channels or degeneration of the dendritic arbor. Kinetic analysis showed no differences in D2-IPSCs in old versus young mice or between sexes. Potentiation of D2-IPSCs by corticotropin releasing factor (CRF) is also conserved in aging, indicating preservation of plasticity mechanisms. These findings have implications for understanding dopamine transmission in aging in both sexes and could explain in part the increased susceptibility of males to SNc degeneration of dopamine neurons in neurodegenerative disorders such as Parkinson’s disease (PD).
Transcriptomic cell type structures in vivo neuronal activity across multiple time scales
Authors: Schneider Aidan , Azabou Mehdi , McDougall-Vigier Louis , Parks David , Ensley Sahara , Bhaskaran-Nair Kiran , Nowakowski Tom , Dyer Eva L. , Hengen Keith B. 
Published: July 2022

SUMMARYCell type is hypothesized to be a key determinant of the role of a neuron within a circuit. However, it is unknown whether a neuron’s transcriptomic type influences the timing of its activity in the intact brain. In other words, can transcriptomic cell type be extracted from the time series of a neuron’s activity? To address this question, we developed a new deep learning architecture that learns features of interevent intervals across multiple timescales (milliseconds to >30 min). We show that transcriptomic cell class information is robustly embedded in the timing of single neuron activity recorded in the intact brain of behaving animals (calcium imaging and extracellular electrophysiology), as well as in a bio-realistic model of visual cortex. In contrast, we were unable to reliably extract cell identity from summary measures of rate, variance, and interevent interval statistics. We applied our analyses to the question of whether transcriptomic subtypes of excitatory neurons represent functionally distinct classes. In the calcium imaging dataset, which contains a diverse set of excitatory Cre lines, we found that a subset of excitatory cell types are computationally distinguishable based upon their Cre lines, and that excitatory types can be classified with higher accuracy when considering their cortical layer and projection class. Here we address the fundamental question of whether a neuron, within a complex cortical network, embeds a fingerprint of its transcriptomic identity into its activity. Our results reveal robust computational fingerprints for transcriptomic types and classes across diverse contexts, defined over multiple timescales.
Loss of FOXP3 function causes expansion of two pools of autoreactive T cells in patients with IPEX syndrome
Authors: Borna Šimon , Lee Esmond , Lakshmanan Uma , Mavers Melissa , Narula Mansi , Ramachandran Akshaya , Baker Jeanette , Schulze Janika , Olek Sven , Marois Louis , Gernez Yael , Bhatia Monica , Bertaina Alice , Roncarolo Maria Grazia , Meffre Eric , Bacchetta Rosa 
Published: July 2022

AbstractThe monogenic autoimmune disease Immunedysregulation polyendocrynopathy entheropathy X-linked syndrome (IPEX) has elucidated the essential function of the transcription factor FOXP3 and of thymic-derived regulatory T (Treg) cells in controlling autoimmunity. However, the presence of autoreactive T cells in IPEX remains undetermined, thus representing a crucial gap in understanding the origin of autoimmunity in a FOXP3 deficient immune system. Combining epigenetic analysis as a lineage marker of Treg identity and TCR sequencing to assess the self-reactive clones, we showed that IPEX patients have two pools of expanded autoreactive T cells. The first originates from the expansion of autoreactive effector T cells (Teff), likely due to loss of Treg suppressive function since it is absent in carrier mothers, in whom Treg cells are functional. The second pool originates, unexpectedly, from Treg cells which lose their phenotypic markers, including CD25 and FOXP3. We call these loss of identity Treg cells and show that they are i) suppressed by healthy donor Treg in a patient post hematopoietic transplantation despite low donor chimerism, and ii) not detectable in patients with Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy syndrome (APECED), a monogenic autoimmune disease of thymic origin. Moreover, we demonstrate that FOXP3 knock-out in Treg cells leads to increased Treg expansion and production of Th17 and Th2 cytokines, known to be increased in IPEX patients. These results suggest that the loss of identity Treg cells could directly contribute to immune dysregulation in IPEX. Collectively, we provide a better understanding of autoimmunity and novel ways to monitor the effects of Treg cell therapies in IPEX disease or other autoimmune diseases.One Sentence SummaryMutations of FOXP3 gene in humans cause expansion of autoreactive T cells originating from both effector T cells and regulatory T cells which gain effector function.
Canadian newspapers support mifepristone medical abortion to improve fulfillment of the right to health (2015-2019)
Authors: Kendall Tamil , Sriram Pallavi , Parmar Amrit , Norman Wendy V. 
Published: July 2022

AbstractIn 2015, mifepristone, the international gold standard for medical abortion, was approved for use in Canada. Our content analysis of Canadian newspaper coverage describes arguments in favor or against medical abortion and the evolving regulatory framework for mifepristone from six months before approval until the last province included the medication as a publicly insured health benefit (2015-2019).Our study found an exceptionally high level of support for the approval, introduction and removal of regulatory barriers to mifepristone for medical abortion. Of 402 articles, 67% were pro-medical abortion, 25% presented balanced or neutral coverage and only 8% presented solely anti-medical abortion viewpoints. Of the 761 stakeholders cited, more than 90% made positive or neutral statements about medical abortion. Most articles discussed medical abortion as a health issue and contained arguments about how liberalization of the regulatory framework and public payment for mifepristone would improve abortion availability (68%), accessibility (87%), acceptability (34%) and quality (19%). Mifepristone’s introduction in Canada was presented in newspapers as a way to increase women’s health, specifically in rural areas where disparities in abortion access exist.Rather than formal balance, which presents contrasting arguments as equally valid even when the scientific evidence for one vastly outweighs the other, our study identified evidentiary balance where coverage aligned with the majority of evidence and expert opinion. Our results differ from analyses in other high-income countries (United Kingdom, United States) that have found that media frame abortion as a stigmatized and controversial issue or mention abortion predominantly with respect to electoral politics rather than as a health issue. The Canadian print media presented overwhelmingly favorable arguments towards the expansion of mifepristone medical abortion and served to destigmatize abortion by framing the introduction and universal coverage of medical abortion as fulfilling core components of the right to health.
Use of illness severity scores to predict mortality in interstitial lung disease patients hospitalised with acute respiratory deterioration
Authors: Williams Rachel L , Hyams Catherine , Robertshaw Joe , Gonzalez Maria Garcia , Szasz-Benczur Zsuzsa , White Paul , Maskell Nick A , Finn Adam , Barratt Shaney L 
Published: July 2022

ABSTRACTIntroductionHospitalisations relating to acute respiratory deteriorations (ARD) in Interstitial Lung Disease (ILD) have poor outcomes. Factors predicting adverse outcomes are not fully understood and data addressing the use of illness severity scores in prognostication are limited.ObjectiveTo investigate the use of CURB-65 and NEWS-2 severity scores in the prediction of mortality following ARD-ILD hospitalisation, using prospective methodology and to validate previously determined cut-offs, derived from a retrospective study cohort.MethodsA dual-centre prospective observational cohort study of all adults (≥18y) hospitalised with ARD-ILD in Bristol, UK (n=179). Gender-Age-Physiology (GAP), CURB-65 and NEWS-2 scores were calculated for each eligible admission.Receiver operating characteristics (ROC) curve analysis was used to quantify the strength of discrimination for NEWS-2 and CURB-65 scores. Univariable and multivariable logistic regression analyses were performed to explore the relationship between baseline severity scores and mortality.ResultsGAP showed some merit at predicting 30-day mortality (AUC=0.64,P=0.015); whereas CURB-65 showed modest predictive value for in-hospital (AUC=0.72,P<0.001) and 90-day mortality (AUC=0.67,P<0.001). NEWS-2 showed higher predictive value for in-hospital (AUC=0.80,P<0.001) and 90-day mortality (AUC=0.75,P<0.001), with an optimal derived cut-off ≥6.5 found to be sensitive and specific for predicting in-hospital (83% and 63%) and 90-day (73% and 72%) mortality. In exploratory analyses, GAP score addition improved the predictive ability of NEWS-2 against 30-day mortality and CURB-65 across all time-periods.ConclusionNEWS-2 has good discriminatory value for predicting in-hospital mortality and moderate discriminatory value for predicting 90-day mortality. The optimal NEWS-2 cut-off value determined was the same as in a previous retrospective cohort, confirming the NEWS-2 score shows promise in predicting mortality following ARD-ILD hospitalisation.
Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture
Authors: Demir-Yilmaz Irem , Schiavone Marion , Esvan Jérôme , Guiraud Pascal , Formosa-Dague Cécile 
Published: July 2022

AbstractThe microalgae cell wall represents its interface with its environment and a strong barrier to disrupt in order to extract the cell’s products. Understanding its composition and architecture is a challenge that if overcome, could lead to substantial advancements in optimizing microalgae-production systems. However, the cell wall is a dynamic and complex structure that evolves depending on the growth phase or culture conditions. To apprehend this complexity, an experimental approach combining AFM, XPS, and chemical hydrolysis followed by HPAEC-PAD was developed to understand the cell wall of Chlorella vulgaris, a biotechnologically-relevant green microalgae species. Exponential and stationary growth stages were investigated, as well as saline stress condition inducing lipid production. Results showed that both the cell wall composition and architecture changes in stationary phase, with an increase of the lipidic fraction at the expanse of the proteic fraction, changes in the polysaccharidic composition, and a significant increase of its rigidity. Under saline stress, cell wall architecture seems to be affected as its rigidity decreases importantly. Altogether, this study demonstrates the power of combining these three techniques to give new insights into C. vulgaris cell wall, in terms of composition and architecture, and of its dynamics in different conditions.
ION CHANNEL THERMODYNAMICS STUDIED WITH TEMPERATURE JUMPS MEASURED AT THE CELL MEMBRANE
Authors: Bassetto Carlos A Z , Pinto Bernardo I , Latorre Ramon , Bezanilla Francisco 
Published: July 2022

AbstractPerturbing the temperature of a system modifies its energy landscape thus providing a ubiquitous tool to understand biological processes. Here, we developed a framework to generate sudden temperature jumps (Tjumps) and sustained temperature steps (Tsteps) to study the temperature dependence of membrane proteins under voltage-clamp, while measuring the membrane temperature. Utilizing the melanin under the Xenopus laevis oocytes membrane as a photothermal transducer, we achieved short Tjumps up to 10 ºC in less than 1.5 ms and constant Tsteps for durations up to 150 ms. We followed the temperature at the membrane with submillisecond time resolution by measuring the time-course of membrane capacitance, which is linearly related to temperature. We applied Tjumps in Kir 1.1b, which reveals a highly temperature-sensitive blockage relief and characterized the effects of Tsteps on the temperature-sensitive channels TRPM8 and TRPV1. These newly developed approaches provide a general tool to study membrane proteins thermodynamics.
Inflammatory sub-phenotypes in sepsis: relationship to outcomes, treatment effect and transcriptomic sub-phenotypes
Authors: Antcliffe David B , Mi Yuxin , Santhakumaran Shalini , Burnham Katie L , Prevost A Toby , Ward Josie K , Marshall Timothy , Bradley Claire , Al-Beidh Farah , Hutton Paula , McKechnie Stuart , Davenport Emma E , Hinds Charles J , O’Kane Cecilia M , McAuley Daniel F , Shankar-Hari Manu , Gordon Anthony C , Knight Julian C 
Published: July 2022

AbstractRationaleHeterogeneity of sepsis limits discovery and targeting of treatments. Clustering approaches in critical illness have identified patient groups who may respond differently to therapies. These include in acute respiratory distress syndrome (ARDS) two inflammatory sub-phenotypes, using latent class analysis (LCA), and in sepsis two Sepsis Response Signatures (SRS), based on transcriptome profiling. It is unknown if inflammatory sub-phenotypes such as those identified in ARDS are present in sepsis and how sub-phenotypes defined with different techniques compare.ObjectivesTo identify inflammatory sub-phenotypes in sepsis using LCA and assess if these show differential treatment responses. These sub-phenotypes were compared to hierarchical clusters based on inflammatory mediators and to SRS sub-phenotypes.MethodsLCA was applied to clinical and biomarker data from two septic shock randomized trials. VANISH compared norepinephrine to vasopressin and hydrocortisone to placebo and LeoPARDS compared levosimendan to placebo. Hierarchical cluster analysis (HCA) was applied to 65, 21 and 11 inflammatory mediators measured in patients from the GAinS (n=124), VANISH (n=155) and LeoPARDS (n=484) studies.Measurements and Main ResultsLCA and HCA identified a sub-phenotype of patients with high cytokine levels and worse organ dysfunction and survival, with no interaction between LCA classes and trial treatment responses. Comparison of inflammatory and transcriptomic sub-phenotypes revealed some similarities but without sufficient overlap that they are interchangeable.ConclusionsA sub-phenotype with high levels of inflammation and increased disease severity is consistently identifiable in sepsis, with similarities to that described in ARDS. There was limited overlap with the transcriptomic sub-phenotypes.
Study on the construction workforce management based on lean construction in the context of COVID-19
Authors: Jiang Lixuan , Zhong Hua , Chen Jianghong , Cheng Jiajia , Chen Shilong , Gong Zili , Lun Zhihui , Zhang Jinhua , Su Zhenmin 
Published: July 2022

PurposeThe construction industry is facing challenges not only for workers' mobility in the pandemic situation but also for Lean Construction (LC) practise in responding to the high-quality development during the post-pandemic. As such, this paper presents a construction workforce management framework based on LC to manage both the emergency goal in migrant worker management and the long-term goal in labour productivity improvement in China.Design/methodology/approachThe framework is created based on the integrated culture and technology strategies of LC. A combination of qualitative and quantitative methods is taken to explore factors influencing the mobility of construction workers and to measure labour productivity improvement. The case study approach is adopted to demonstrate the framework application.FindingsFor method application, a time-and-motion study and Percent Plan Complete indicator are proposed to offer labour productivity measurements of “resources efficiency” and “flow efficiency”. Moreover, the case study provides an industry level solution for construction workforce management and Lean Construction culture shaping, as well as witnesses the LC culture and technology strategies alignment contributing to LC practise innovation.Originality/valueCompared with previous studies which emphasised solely LC techniques rather than socio-technical system thinking, the proposed integration framework as well as implementation of “Worker's Home” and “Lean Work Package” management models in the COVID-19 pandemic contribute to new extensions of both the fundamental of knowledge and practise in LC.
Predictive model assessment and selection in composite-based modeling using PLS-SEM: extensions and guidelines for using CVPAT
Authors: Sharma Pratyush N. , Liengaard Benjamin D. , Hair Joseph F. , Sarstedt Marko , Ringle Christian M. 
Published: July 2022

Purpose
Researchers often stress the predictive goals of their partial least squares structural equation modeling (PLS-SEM) analyses. However, the method has long lacked a statistical test to compare different models in terms of their predictive accuracy and to establish whether a proposed model offers a significantly better out-of-sample predictive accuracy than a naïve benchmark. This paper aims to address this methodological research gap in predictive model assessment and selection in composite-based modeling.

Design/methodology/approach
Recent research has proposed the cross-validated predictive ability test (CVPAT) to compare theoretically established models. This paper proposes several extensions that broaden the scope of CVPAT and explains the key choices researchers must make when using them. A popular marketing model is used to illustrate the CVPAT extensions’ use and to make recommendations for the interpretation and benchmarking of the results.

Findings
This research asserts that prediction-oriented model assessments and comparisons are essential for theory development and validation. It recommends that researchers routinely consider the application of CVPAT and its extensions when analyzing their theoretical models.

Research limitations/implications
The findings offer several avenues for future research to extend and strengthen prediction-oriented model assessment and comparison in PLS-SEM.

Practical implications
Guidelines are provided for applying CVPAT extensions and reporting the results to help researchers substantiate their models’ predictive capabilities.

Originality/value
This research contributes to strengthening the predictive model validation practice in PLS-SEM, which is essential to derive managerial implications that are typically predictive in nature.