bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal trends in produce purchasing behavior: a descriptive study of transaction level data from loyalty card households
Authors: Fernandez Isabel Diana , Johnson Brent A. , Wixom Nellie , Kautz Amber , Janciuras Joanne , Prevost Steve , Luo Jiebo , Ramchandran Rajeev S. 
Published: November 2022

AbstractBackgroundHousehold food purchases (HFP) are in the pathway between the community food environment and the foods available in households for consumption. As such, HFP data have emerged as alternatives to monitor population dietary trends over-time. In this paper, we investigate the use of loyalty card datasets as unexplored sources of continuously collected HFP data to describe temporal trends in household produce purchases.MethodsWe partnered with a grocery store chain to obtain a loyalty card database with grocery transactions by household from January 2016-October 2018. We included households in an urban county with complete observations for head of household age group, household income group, and family size. Data were summarized as weighted averages (95% CI) of percent produce purchased out of all foods purchased by household per month. We modeled seasonal and linear trends in the proportion of produce purchases by age group and income while accounting for repeated observations per household using generalized estimating equations.ResultsThere are 290,098 households in the database (88% of all county households). At baseline, the smallest and largest percent produce purchases are observed among the youngest and lowest income (12.2%, CI 11.1; 13.3) and the oldest and highest income households (19.3, CI 18.9; 19.6); respectively. The seasonal variations are consistent in all age and income groups with an April-June peak gradually descending until December. However, the average linear change in percent produce purchased per household per year varies by age and income being the steepest among the youngest households at each income level (from 1.42%, CI 0.98;1.8 to 0.69%, CI 0.42;0.95) while the oldest households experience almost no annual change.Conclusions We explored the potential of a collaboration with a food retailer to use continuously collected loyalty card data for public health nutrition purposes. Our findings suggest a trend towards a healthier pattern in long-term food purchases and household food availability among the youngest households that may lessen the population chronic disease burden if sustained. Understanding the foods available for consumption within households allows public health advocates to develop and evaluate policies and programs promoting foods and nutrients along the life course.
Modification of mesenchymal stem cells for cartilage-targeted therapy
Authors: Huang Jianghong , Liu Qisong , Xia Jiang , Chen Xi , Xiong Jianyi , Yang Lei , Liang Yujie 
Published: November 2022

AbstractOsteoarthritis (OA) is a chronic degenerative joint disease characterized by the destruction of the articular cartilage, sclerosis of the subchondral bone, and joint dysfunction. Its pathogenesis is attributed to direct damage and mechanical destruction of joint tissues. Mesenchymal stem cells (MSCs), suggested as a potential strategy for the treatment of OA, have shown therapeutic effects on OA. However, the specific fate of MSCs after intraarticular injection, including cell attachment, proliferation, differentiation, and death, is still unclear, and there is no guarantee that stem cells can be retained in the cartilage tissue to enact repair. Direct homing of MSCs is an important determinant of the efficacy of MSC-based cartilage repair. Recent studies have revealed that the unique homing capacity of MSCs and targeted modification can improve their ability to promote tissue regeneration. Here, we comprehensively review the homing effect of stem cells in joints and highlight progress toward the targeted modification of MSCs. In the future, developments of this targeting system that accelerate tissue regeneration will benefit targeted tissue repair.Graphical Abstract
Cladribine treatment improves cortical network functionality in a mouse model of autoimmune encephalomyelitis
Authors: Schroeter Christina B. , Rolfes Leoni , Gothan K. S. Sophie , Gruchot Joel , Herrmann Alexander M. , Bock Stefanie , Fazio Luca , Henes Antonia , Narayanan Venu , Pfeuffer Steffen , Nelke Christopher , Räuber Saskia , Huntemann Niklas , Duarte-Silva Eduardo , Dobelmann Vera , Hundehege Petra , Wiendl Heinz , Raba Katharina , Küry Patrick , Kremer David , Ruck Tobias , Müntefering Thomas , Budde Thomas , Cerina Manuela , Meuth Sven G. 
Published: November 2022

Abstract
Background
Cladribine is a synthetic purine analogue that interferes with DNA synthesis and repair next to disrupting cellular proliferation in actively dividing lymphocytes. The compound is approved for the treatment of multiple sclerosis (MS). Cladribine can cross the blood–brain barrier, suggesting a potential effect on central nervous system (CNS) resident cells. Here, we explored compartment-specific immunosuppressive as well as potential direct neuroprotective effects of oral cladribine treatment in experimental autoimmune encephalomyelitis (EAE) mice.

Methods
In the current study, we compare immune cell frequencies and phenotypes in the periphery and CNS of EAE mice with distinct grey and white matter lesions (combined active and focal EAE) either orally treated with cladribine or vehicle, using flow cytometry. To evaluate potential direct neuroprotective effects, we assessed the integrity of the primary auditory cortex neuronal network by studying neuronal activity and spontaneous synaptic activity with electrophysiological techniques ex vivo.

Results
Oral cladribine treatment significantly attenuated clinical deficits in EAE mice. Ex vivo flow cytometry showed that cladribine administration led to peripheral immune cell depletion in a compartment-specific manner and reduced immune cell infiltration into the CNS. Histological evaluations revealed no significant differences for inflammatory lesion load following cladribine treatment compared to vehicle control. Single cell electrophysiology in acute brain slices was performed and showed an impact of cladribine treatment on intrinsic cellular firing patterns and spontaneous synaptic transmission in neurons of the primary auditory cortex. Here, cladribine administration in vivo partially restored cortical neuronal network function, reducing action potential firing. Both, the effect on immune cells and neuronal activity were transient.

Conclusions
Our results indicate that cladribine exerts a neuroprotective effect after crossing the blood–brain barrier independently of its peripheral immunosuppressant action.
Whole-genome sequence analysis reveals the circulation of multiple SARS-CoV-2 variants of concern in Nairobi and neighboring counties, Kenya between March and July 2021
Authors: Khamadi Samoel Ashimosi , Opanda Silvanos , Symekher Samwel Lifumo , Limbaso Samson Konongoi , Langat Solomon , Cirindi Josyline Kaburi , Mwangi Milkah , Mwikwabe Nicholas , Okeyo Seth , Koskei Edith , Mutisya James , Owaka Samwel , Nyunja Albert , Koka Hellen , Wadegu Meshack , Chitechi Esther , Achilla Rachel , Majanja Janet Masitsa , Kanyara Lucy , Amukoye Evans , Bulimo Wallace 
Published: November 2022

AbstractThe emergence and rapid spread of SARS-CoV-2 variants of concern (VOC) have been linked to new waves of COVID-19 epidemics occurring in different regions of the world. The VOC have acquired adaptive mutations that have enhanced virus transmissibility, increased virulence, and reduced response to neutralizing antibodies. Kenya has experienced six waves of COVID-19 epidemics. In this study, we analyzed 64 genome sequences of SARS-CoV-2 strains that circulated in Nairobi and neighboring counties, Kenya between March 2021 and July 2021. Viral RNA was extracted from RT-PCR confirmed COVID-19 cases, followed by sequencing using the ARTIC network protocol and Oxford Nanopore Technologies. Analysis of the sequence data was performed using different bioinformatics methods. Our analyses revealed that during the study period, three SARS-CoV-2 variants of concern (VOC) circulated in Nairobi and nearby counties in Kenya. The Alpha (B.1.1.7) lineage predominated (62.7%), followed by Delta (B.1.617.2, 35.8%) and Beta (B.1.351, 1.5%). Notably, the Alpha (B.1.1.7) VOC were most frequent from March 2021 to May 2021, while the Delta (B.1.617.2) dominated beginning June 2021 through July 2021. Sequence comparisons revealed that all the Kenyan viruses were genetically similar to those that circulated in other regions. Although the majority of Kenyan viruses clustered together in their respective phylogenetic lineages/clades, a significant number were interspersed among foreign strains. Between March and July 2021, our study's findings indicate the prevalence of multiple lineages of SAR-CoV-2 VOC in Nairobi and nearby counties in Kenya. The data suggest that the recent increase in SARS-CoV-2 infection, particularly in Nairobi and Kenya as a whole, is attributable to the introduction and community transmission of SARS-CoV-2 VOC among the populace. In conclusion, the findings provide a snapshot of the SARS-CoV-2 variants that circulated in Kenya during the study period.
Clinical correlates of women endorsing premenstrual suicidal ideation: a cross-sectional study
Authors: Carlini Sara V. , Weiss Sandra J. , Mordukhaev Lauren , Jacob Sunu , Flynn Heather A. , Deligiannidis Kristina M. 
Published: November 2022

Abstract
Background
Prevalence of premenstrual syndrome (PMS) may be as high as 13-18%, but it remains under-recognized and is associated with increased suicidal ideation (SI), plans, and attempts in epidemiological studies. The present study reports on women endorsing premenstrual SI (PMSI) and characterizes this at-risk group and its clinical correlates.

Methods
A cross-sectional study assessed demographics, anxiety and depression severity, psychiatric diagnoses, menstrual symptoms, SI, and trauma in adult women at a major medical center over 11 months.

Results
Three hundred two women were assessed. Of 153 participants endorsing premenstrual symptoms, 41 (27%) reported new or worsening concurrent premenstrual passive or active SI. Women who reported PMSI were significantly more likely to be single, unemployed, and childless as well as significantly more likely to report interference from premenstrual symptoms, histories of psychiatric hospitalization, adverse childhood events, suicide attempts, and current and past depression and anxiety compared to women without PMSI. The final regression model indicated the most significant predictors of PMSI were history of a depression diagnosis, severity of current depressive symptoms, and having experienced 3 or more childhood adverse events.

Conclusion
Nearly one-third of women reporting premenstrual symptoms endorsed concurrent SI, a clinically valuable demonstration of the importance of this predictable cyclic risk factor.
Lineage abundance estimation for SARS-CoV-2 in wastewater using transcriptome quantification techniques
Authors: Baaijens Jasmijn A. , Zulli Alessandro , Ott Isabel M. , Nika Ioanna , van der Lugt Mart J. , Petrone Mary E. , Alpert Tara , Fauver Joseph R. , Kalinich Chaney C. , Vogels Chantal B. F. , Breban Mallery I. , Duvallet Claire , McElroy Kyle A. , Ghaeli Newsha , Imakaev Maxim , Mckenzie-Bennett Malaika F. , Robison Keith , Plocik Alex , Schilling Rebecca , Pierson Martha , Littlefield Rebecca , Spencer Michelle L. , Simen Birgitte B. ,   Yale SARS-CoV-2 Genomic Surveillance Initiative, Altajar Ahmad , Brito Anderson F. , Watkins Anne E. , Muyombwe Anthony , Neal Caleb , Liu Chen , Castaldi Christopher , Pearson Claire , Peaper David R. , Laszlo Eva , Tikhonova Irina R. , Razeq Jafar , Rothman Jessica E. , Wang Jianhui , Bilguvar Kaya , Niccolai Linda , Wilson Madeline S. , Anderson Margaret L. , Landry Marie L. , Adams Mark D. , Hui Pei , Downing Randy , Earnest Rebecca , Mane Shrikant , Murphy Steven , Hanage William P. , Grubaugh Nathan D. , Peccia Jordan , Baym Michael 
Published: November 2022

AbstractEffectively monitoring the spread of SARS-CoV-2 mutants is essential to efforts to counter the ongoing pandemic. Predicting lineage abundance from wastewater, however, is technically challenging. We show that by sequencing SARS-CoV-2 RNA in wastewater and applying algorithms initially used for transcriptome quantification, we can estimate lineage abundance in wastewater samples. We find high variability in signal among individual samples, but the overall trends match those observed from sequencing clinical samples. Thus, while clinical sequencing remains a more sensitive technique for population surveillance, wastewater sequencing can be used to monitor trends in mutant prevalence in situations where clinical sequencing is unavailable.
AGI grade-guided chaiqin chengqi decoction treatment for predicted moderately severe and severe acute pancreatitis (CAP trial): study protocol of a randomised, double-blind, placebo-controlled, parallel-group, pragmatic clinical trial
Authors: Chen Zhiyao , Yang Xiaonan , Guo Jia , Jin Tao , Lin Ziqi , Zhu Ping , Li Jing , Li Ling , Sun Xin , Du Dan , Jiang Kun , He Yanqiu , Cai Fei , Li Lan , Hu Cheng , Tan Qingyuan , Huang Wei , Deng Lihui , Xia Qing 
Published: November 2022

Abstract
Background
Acute pancreatitis (AP) is a common digestive disease with increased incidence globally but without internationally licenced pharmacological therapy. Moderately severe and severe acute pancreatitis (MSAP/SAP) contributes predominately for its morbidities and mortality and has been managed in West China Hospital for decades using the traditional Chinese medicinal formula chaiqin chengqi decoction (CQCQD). The current study tests whether the early administration of CQCQD will result in improved clinical outcomes in predicted MSAP/SAP patients.

Methods
This is a single-centre, randomised, controlled, double-blind pragmatic clinical trial. AP patients aged 18–75 admitted within 72 h of onset will be assessed at admission for enrolment. We excluded the predicted mild acute pancreatitis (Harmless Acute Pancreatitis Score > 2 at admission) and severe organ failure (Sequential Organ Failure Assessment [SOFA] score of respiratory, cardiovascular, or renal systems > 3) at admission. Eligible patients will be randomly allocated on a 1:1 basis to CQCQD or placebo control administration based on conventional therapy. The administration of CQCQD and placebo is guided by the Acute Gastrointestinal Injury grade-based algorithm. The primary outcome measure will be the duration of respiratory failure (SOFA score of respiratory system ≥ 2) within 28 days after onset. Secondary outcome measures include occurrence of new-onset any organ failure (SOFA score of respiratory, cardiovascular, or renal system ≥ 2) and new-onset persistent organ failure (organ failure lasts > 48 h), dynamic surrogate biochemical markers and clinical severity scores, gut-centred treatment modalities, local complications status, intensive care need and duration, surgical interventions, mortality, and length of hospital stay. Follow-up will be scheduled on 6, 12, and 26 weeks after enrolment to assess AP recurrence, local complications, the requirement for surgical interventions, all-cause mortality, and patient-reported outcomes.

Discussion
The results of this study will provide high-quality evidence to appraise the efficacy of CQCQD for the early management of AP patients.

Trial registration
Chictr.org.cn Registry (ChiCTR2000034325). Registered on 2 July, 2020.
The mixed liver and kidney transcriptome dataset of Darevskia valentini rock lizard
Authors: Ryakhovsky Sergei S. , Zhernakova Daria V. , Korchagin Vitaly I. , Vergun Andrey A. , Girnyk Anastasiya E. , Dikaya Victoria A. , Arakelyan Marine S. , Komissarov Aleksey S. , Ryskov Alexey P. 
Published: November 2022

Abstract
Objectives
This study is performed in the frame of a bigger study dedicated to genomics and transcriptomics of parthenogenesis in vertebrates. Among vertebrates, obligate parthenogenesis was first described in the lizards of the genus Darevskia. In this genus, all found parthenogenetic species originated via interspecific hybridization. It remains unknown which genetic or genomic factors play a key role in the generation of parthenogenetic organisms. Comparative genomic and transcriptomic analysis of parthenogens and their parental species may elucidate this problem. Darevskia valentini is a paternal species for four (of seven) parthenogens of this genus, which we promote as a particularly important species for the generation of parthenogenetic forms.

Data description
Total cellular RNA was isolated from kidney and liver tissues using the standard Trizol Tissue RNA Extraction protocol. Sequencing of transcriptome libraries prepared by random fragmentation of cDNA samples was performed on an Illumina HiSeq2500. Obtained raw sequences contained 117,6 million reads with the GC content of 47%. After preprocessing, raw data was assembled by Trinity and produced 491,482 contigs.
Chest wall loading in the ICU: pushes, weights, and positions
Authors: Selickman John , Marini John J. 
Published: November 2022

AbstractClinicians monitor mechanical ventilatory support using airway pressures—primarily the plateau and driving pressure, which are considered by many to determine the safety of the applied tidal volume. These airway pressures are influenced not only by the ventilator prescription, but also by the mechanical properties of the respiratory system, which consists of the series-coupled lung and chest wall. Actively limiting chest wall expansion through external compression of the rib cage or abdomen is seldom performed in the ICU. Recent literature describing the respiratory mechanics of patients with late-stage, unresolving, ARDS, however, has raised awareness of the potential diagnostic (and perhaps therapeutic) value of this unfamiliar and somewhat counterintuitive practice. In these patients, interventions that reduce resting lung volume, such as loading the chest wall through application of external weights or manual pressure, or placing the torso in a more horizontal position, have unexpectedly improved tidal compliance of the lung and integrated respiratory system by reducing previously undetected end-tidal hyperinflation. In this interpretive review, we first describe underappreciated lung and chest wall interactions that are clinically relevant to both normal individuals and to the acutely ill who receive ventilatory support. We then apply these physiologic principles, in addition to published clinical observation, to illustrate the utility of chest wall modification for the purposes of detecting end-tidal hyperinflation in everyday practice.
Using tensor contractions to derive the structure of slice-wise multiplications of tensors with applications to space–time Khatri–Rao coding for MIMO-OFDM systems
Authors: Naskovska Kristina , Sokal Bruno , de Almeida André L. F. , Haardt Martin 
Published: November 2022

AbstractThe slice-wise multiplication of two tensors is required in a variety of tensor decompositions (including PARAFAC2 and PARATUCK2) and is encountered in many applications, including the analysis of multidimensional biomedical data (EEG, MEG, etc.) or multi-carrier multiple-input multiple-output (MIMO) systems. In this paper, we propose a new tensor representation that is not based on a slice-wise (matrix) description, but can be represented by a double contraction of two tensors. Such a double contraction of two tensors can be efficiently calculated via generalized unfoldings. It leads to new tensor models of the investigated system that do not depend on the chosen unfolding (in contrast to matrix models) and reveal the tensor structure of the data model, such that all possible unfoldings can be seen at the same time. As an example, we apply this new concept to the design of new receivers for multi-carrier MIMO systems in wireless communications. In particular, we consider MIMO-orthogonal frequency division multiplexing (OFDM) systems with and without Khatri–Rao coding. The proposed receivers exploit the channel correlation between adjacent subcarriers, require the same amount of training symbols as traditional OFDM techniques, but have an improved performance in terms of the symbol error rate. Furthermore, we show that the spectral efficiency of the Khatri–Rao-coded MIMO-OFDM can be increased by introducing cross-coding such that the “coding matrix” also contains useful information symbols. Considering this transmission technique, we derive a tensor model and two types of receivers for cross-coded MIMO-OFDM systems using the double contraction of two tensors.
Reducing interference via link adaptation in delay-critical wireless networks
Authors: Mandelli Silvio , Lieto Alessandro , Razenberg Mark , Weber Andreas , Wild Thorsten 
Published: November 2022

AbstractOne of the current (6G) wireless networks research’s trends is to investigate short distance and dense scenarios, where users are locally connected in sub-networks. Such use case is critical to support the advances of industrial internet of things or Industry 4.0 (I4.0), e.g. connecting an entire group of sensors and actuators of a robot. Therefore, schemes that can properly manage the interference must be deployed in practical systems to allow the promised performance advances of 6G. Targeting these high density scenarios, we describe the Power Optimization for Low Interference and Throughput Enhancement (POLITE) paradigm for link adaptation and power allocation, which leverages available radio resources to stabilize and reduce the interference. The baseline link adaptation schemes are compared with POLITE in their performance in a 3rd generation partnership project (3GPP) calibrated system level simulator for industrial scenarios. As services in industrial environments require high reliability under constrained delays, we propose different delay-aware formulations in the POLITE design. In this work, we provide solutions both for relaxed delay requirements and for latency critical traffic, whose delay must be minimized. In particular, in the latter case, we propose also modifications of user selection and resource allocation procedures to further improve the reliability and latency. Simulation results prove the benefits of POLITE in terms of increased throughput, fulfillment of relaxed and delay-critical requirements, with an overall reduced transmit power compared to the current baseline link adaptation schemes.