bluuebunny/crossref_metadata_2025_split huggingface dataset:
CAPturAM, a Chemo‐Enzymatic Strategy for Selective Enrichment and Detection of Physiological CAPAM‐Targets
Authors: Muthmann Nils , Albers Marvin , Rentmeister Andrea 
Published: October 2022

AbstractModified nucleotides impact all aspects of eukaryotic mRNAs and contribute to regulation of gene expression at the transcriptional and translational level. At the 5′ cap, adenosine as first transcribed nucleotide is often N6‐methyl‐2′‐O‐methyl adenosine (m6Am). This modification is tissue dependent and reversible, pointing to a regulatory function. CAPAM was recently identified as methyltransferase responsible for m6Am formation, however, the direct assignment of its target transcripts proves difficult. Antibodies do not discriminate between internal N6‐methyl adenosine (m6A) and m6Am. Here we present CAPturAM, an antibody‐free chemical biology approach for direct enrichment and probing of physiological CAPAM‐targets. We harness CAPAM's cosubstrate promiscuity to install propargyl groups on its targets. Subsequent functionalization with an affinity handle allows for their enrichment. Using wildtype and CAPAM−/− cells, we successfully applied CAPturAM to confirm or disprove CAPAM‐targets, facilitating the verification and identification of CAPAM targets.
Synthesis and Biological Evaluation of C(13)/C(13′)‐Bis(desmethyl)disorazole Z**
Authors: Bold Christian Paul , Lucena‐Agell Daniel , Oliva María Ángela , Díaz José Fernando , Altmann Karl‐Heinz 
Published: October 2022

AbstractWe describe the total synthesis of the macrodiolide C(13)/C(13′)‐bis(desmethyl)disorazole Z through double inter‐/intramolecular Stille cross‐coupling of a monomeric vinyl stannane/vinyl iodide precursor to form the macrocycle. The key step in the synthesis of this precursor was a stereoselective aldol reaction of a formal Evans acetate aldol product with crotonaldehyde. As demonstrated by X‐ray crystallography, the binding mode of C(13)/C(13′)‐bis(desmethyl)disorazole Z to tubulin is virtually identical with that of the natural product disorazole Z. Likewise, C(13)/C(13′)‐bis(desmethyl)disorazole Z inhibits tubulin assembly with at least the same potency as disorazole Z and it appears to be a more potent cell growth inhibitor.
High‐Performance All‐Solid‐State Proton Rectifier Using a Heterogeneous Membrane Composed of Coordination Polymer and Layered Double Hydroxide
Authors: Lu Jiangfeng , Yoshida Yukihiro , Maesato Mitsuhiko , Kitagawa Hiroshi 
Published: October 2022

AbstractRational control of unidirectional proton transport is highly challenging, primarily owing to the difficulty in introducing an asymmetric factor into proton conducting media. In this study, free‐standing membranes of a proton‐conducting two‐dimensional porous coordination polymer, Cu2(CuTCPP) (H2TCPP: 5,10,15,20‐tetrakis(4‐carboxyphenyl)porphyrin) and a hydroxide ion‐conducting layered double hydroxide, Mg‐Al‐LDH(NO3), were combined to generate a pH gradient in the conducting media. The current–voltage measurements revealed that the heterogeneous membrane exhibits a significant unidirectional proton transport with a proton rectification ratio exceeding 200 under 90 % relative humidity in the initial voltage scan. This value is the highest among the reported all‐solid‐state proton rectifiers. The high designability of both components with well‐defined structures, which is in contrast to the organic polymers used so far, provides a new avenue for developing and understanding the proton‐rectifying behavior in the solid state.
The influence of addition of carbon nanotube and graphene platelets on characteristics of carbon/basalt fiber reinforced intra‐ply hybrid composites
Authors: Azimpour‐Shishevan Farzin , Mohtadi‐Bonab M. A. , Akbulut Hamit 
Published: October 2022

AbstractIn this study, effects of addition of carbon nanotubes (CNTs) and graphene platelets (GPLs) on characteristics of carbon/basalt fiber reinforced intra‐ply hybrid composites were investigated. The composites were fabricated using vacuum assisted resin infusion molding (VARIM) method in two types including bare and 0.1, 0.5 wt.% of GPL and CNT nanoparticles filled hybrid composites. Fabricated normal and multiscale composites were cut by water jet and mechanical properties of specimens were examined by tensile, flexural, SBS experiments. Therefore, the modulus of elasticity, flexural modulus, tensile and flexural strength and ILSS of bare and multiscale composites were compared. Thermomechanical properties of fabricated composites were evaluated by dynamic mechanic analyze (DMA), thermogravimetric analyze(TGA) and thermal conductivity (TC) tests and storage modulus, loss modulus, damping ratio, glass transition temperature, weight loss and derivative weight loss were compared in fabricated normal and multiscale composites. Similarly, modal properties of fabricated composites such as natural frequency and damping factor were obtained by vibrational tests and compared in fabricated composites. According to the results, the addition of carbon‐based nanoparticles improved the characteristics of carbon/basalt fiber intra‐ply hybrid composites. The response of composites was directly proportional to the addition ratio of the carbon‐based nanoparticles.
Atomically Dispersed Mn for Electrochemical CO2 Reduction with Tunable Performance
Authors: Yao Zhibo , Zhan Xinyu , Ruan Yukun , Li Weixiang , Xu Yiwen , Chen Yuxin , Robertson Alex W. , Tao Ranting , Hong Song , Hao Leiduan , Sun Zhenyu 
Published: October 2022

AbstractElectrochemical CO2 reduction (ECR) is recognized as a sustainable and promising approach for the production of high‐value chemicals. To facilitate widespread application of this technology, the design and construction of efficient cathodic electrocatalysts is critically important. Here we report the synthesis of atomically dispersed manganese on nitrogen‐doped porous carbon (Mn SAs/NC) using a facile and scalable annealing method for catalyzing the ECR reaction. The as‐obtained Mn SAs/NC delivers high activity and selectivity toward CO formation with a faradaic efficiency of 80.5±0.6%, over 5 times that of bare NC. The high activity is preserved even after 10 h of continuous polarization. The catalytic properties of our cost‐effective Mn SAs/NC catalyst are readily tuned by regulating the nitrogen configurations and the percentage of Mn SAs via modulation of the nitrogen precursor and the thermal treatment conditions.
The roles ofMMP8/MMP10polymorphisms in ischemic stroke susceptibility
Authors: Zhao Yong , Zhang Qi , Zhang Xiaobo , Zhang Yu , Lu Ying , Ma Xiaojuan , Li Weiping , Niu Xiaochen , Zhang Gejuan , Chang Mingze , Shi Wenzhen , Tian Ye 
Published: October 2022

AbstractBackgroundIschemic stroke (IS), a multifactorial and polygenic disease, is the most common cause of death. This study aimed to determine the roles ofMMP8/MMP10polymorphisms in IS susceptibility in the Chinese Han population.MethodsMMP8rs1940475 and rs3765620, andMMP10rs17860949 from 700 IS patients and 700 controls were genotyped by the MassARRAY iPLEX platform. The impact of polymorphisms on IS risk was evaluated by logistic regression analysis.ResultsOur study indicated that rs17860949 inMMP10was significantly associated with a reduced risk of IS (OR = 0.632,p= .002). Precisely, stratification analysis showed that rs17860949 was relate to a decreased susceptibility to IS in patients aged > 55 years (OR = 0.472,p< .001), males (OR = 0.632,p= .012), nonsmokers (OR = 0.610,p= .017), and nondrinkers (OR = 0.559,p= .006). All these significant findings were verified by false‐positive report probability test. Furthermore, GG genotype and AG genotype inMMP8rs3765620 polymorphism were related to a reduced triglycerides concentration (p= .018).ConclusionOur study suggests that rs17860949 inMMP10may play a protective role in IS in the Chinese Han population.
Fine needle aspiration of pancreatic lesions focusing on secondary tumors with emphasis of metastatic breast cancer: A clinicopathological study with follow‐up
Authors: Chen Maria Yanqing , Zarrin‐Khameh Neda , Xu Ya 
Published: October 2022

AbstractBackgroundThe data on metastatic tumors to the pancreas diagnosed by fine needle aspiration (FNA) biopsy is limited. We report our experience of FNA of primary and secondary pancreatic tumors emphasizing metastatic breast cancer in the pancreas.MethodTotal 274 cases of pancreatic FNA in 10 years were retrospectively reviewed. Literature review of metastatic breast cancers to the pancreas was performed.ResultsOut of the 274 cases, 7 (7/274, 2.6%) cases were non‐diagnostic, 46 (46/274, 16.8%) cases were negative for malignancy, and 40 (40/274, 14.6%) cases were under the category of atypical cells. There were 133 (133/274, 48.5%) cases diagnosed as positive for malignancy, 20 (20/274, 7.3%) suspicious for malignancy, and 28 (28/274, 10.2%) cases in the category of neoplastic: other. The most common neoplasm diagnosed was ductal adenocarcinoma (114/274, 41.6%; 114/133, 85.7% in positive for malignancy category). Ten cases (10/274, 3.7%) were diagnosed as metastatic neoplasms to the pancreas, including four breast infiltrating ductal carcinomas (IDC), one endocervical adenocarcinoma, one anal/rectal squamous cell carcinoma, one renal cell carcinoma, one hepatocellular carcinoma, one seminoma and one lung adenocarcinoma. We summarized the biomarkers of the four metastatic breast cancers and conducted literature review on biomarkers of metastatic breast cancers to the pancreas.ConclusionsUpon analyzing FNAs of primary and secondary tumors in the pancreas, we have found breast carcinoma is the most common secondary pancreatic neoplasm in our patient population. Triple negative breast ductal carcinoma is the most common tumor among the metastasis of breast carcinomas to the pancreas. To the best of our knowledge, this study is the first report with a literature review focusing on biomarkers of metastatic breast cancer to the pancreas.
Inverse In Vitro Selection Enables Comprehensive Analysis of Cross‐Chiral L‐Aptamer Interactions
Authors: Piwko Alexander T. , Han Xuan , Kabza Adam M. , Dey Sougata , Sczepanski Jonathan T. 
Published: October 2022

AbstractAptamers composed of mirror‐image L‐(deoxy)ribose nucleic acids, referred to as L‐aptamers, are a promising class of RNA‐binding reagents. Yet, the selectivity of cross‐chiral interactions between L‐aptamers and their RNA targets remain poorly characterized, limiting the potential utility of this approach for applications in biological systems. Herein, we carried out the first comprehensive analysis of cross‐chiral L‐aptamer selectivity using a newly developed “inverse” in vitro selection approach that exploits the genetic nature of the D‐RNA ligand. By employing a library of more than a million target‐derived sequences, we determined the RNA sequence and structural preference of a model L‐aptamer and revealed previously unidentified and potentially broad off‐target RNA binding behaviors. These results provide valuable information for assessing the likelihood and consequences of potential off‐target interactions and reveal strategies to mitigate these effects. Thus, inverse in vitro selection provides several opportunities to advance L‐aptamer technology.
The Osteosarcoma Stem Cell Activity of a Gallium(III)‐Phenanthroline Complex Appended to Salicylate
Authors: Vincent Ruby A. , Passeri Ginevra , Northcote‐Smith Joshua , Singh Kuldip , Suntharalingam Kogularamanan 
Published: October 2022

AbstractWe report the synthesis, characterisation, and anti‐osteosarcoma properties of a gallium(III) complex (1) comprising of two 1,10‐phenanthroline ligands and salicylate, a non‐steroidal anti‐inflammatory drug. The gallium(III) complex 1 displays micromolar potency towards bulk osteosarcoma cells and osteosarcoma stem cells (OSCs). Notably, the gallium(III) complex 1 exhibits significantly higher toxicity towards OSCs grown in monolayer and three‐dimensional cultures than cisplatin, a frontline anti‐osteosarcoma drug. Nuclei isolation and immunoblotting studies show that the gallium(III) complex 1 enters osteosarcoma cell nuclei and induces DNA damage. Flow cytometry and cytotoxicity studies (in the presence of prostaglandin E2) indicate that the gallium(III) complex 1 downregulates cyclooxygenase‐2 (COX‐2) expression and kills osteosarcoma cells in a COX‐2‐dependent manner. Further, the mode of osteosarcoma cell death evoked by the gallium(III) complex 1 is characterised as caspase‐dependent apoptosis.
Concentrated ternary ether electrolyte allows for stable cycling of a lithium metal battery with commercial mass loading high‐nickel NMC and thin anodes
Authors: Yang Jun , Li Xing , Qu Ke , Wang Yixian , Shen Kangqi , Jiang Changhuan , Yu Bo , Luo Pan , Li Zhuangzhi , Chen Mingyang , Guo Bingshu , Wang Mingshan , Chen Junchen , Ma Zhiyuan , Huang Yun , Yang Zhenzhong , Liu Pengcheng , Huang Rong , Ren Xiaodi , Mitlin David 
Published: October 2022

AbstractA new concentrated ternary salt ether‐based electrolyte enables stable cycling of lithium metal battery (LMB) cells with high‐mass‐loading (13.8 mg cm−2, 2.5 mAh cm−2) NMC622 (LiNi0.6Co0.2Mn0.2O2) cathodes and 50 μm Li anodes. Termed “CETHER‐3,” this electrolyte is based on LiTFSI, LiDFOB, and LiBF4 with 5 vol% fluorinated ethylene carbonate in 1,2‐dimethoxyethane. Commercial carbonate and state‐of‐the‐art binary salt ether electrolytes were also tested as baselines. With CETHER‐3, the electrochemical performance of the full‐cell battery is among the most favorably reported in terms of high‐voltage cycling stability. For example, LiNixMnyCo1–x–yO2 (NMC)‐Li metal cells retain 80% capacity at 430 cycles with a 4.4 V cut‐off and 83% capacity at 100 cycles with a 4.5 V cut‐off (charge at C/5, discharge at C/2). According to simulation by density functional theory and molecular dynamics, this favorable performance is an outcome of enhanced coordination between Li+ and the solvent/salt molecules. Combining advanced microscopy (high‐resolution transmission electron microscopy, scanning electron microscopy) and surface science (X‐ray photoelectron spectroscopy, time‐of‐fight secondary ion mass spectroscopy, Fourier‐transform infrared spectroscopy, Raman spectroscopy), it is demonstrated that a thinner and more stable cathode electrolyte interphase (CEI) and solid electrolyte interphase (SEI) are formed. The CEI is rich in lithium sulfide (Li2SO3), while the SEI is rich in Li3N and LiF. During cycling, the CEI/SEI suppresses both the deleterious transformation of the cathode R‐3m layered near‐surface structure into disordered rock salt and the growth of lithium metal dendrites.
Asymptotic theory in bipartite graph models with a growing number of parameters
Authors: Fan Yifan , Jiang Binyan , Yan Ting , Zhang Yuan 
Published: October 2022

AbstractAffiliation networks contain a set of actors and a set of events, where edges denote the affiliation relationships between actors and events. Here, we introduce a class of affiliation network models for modelling the degree heterogeneity, where two sets of degree parameters are used to measure the activeness of actors and the popularity of events, respectively. We develop the moment method to infer these degree parameters. We establish a unified theoretical framework in which the consistency and asymptotic normality of the moment estimator hold as the numbers of actors and events both go to infinity. We apply our results to several popular models with weighted edges, including generalized ‐, Poisson and Rayleigh models. Simulation studies and a realistic example that involves the Poisson model provide concrete evidence that supports our theoretical findings.
The influence from a demand perspective with real economic activity: China versus the United States in world oil markets
Authors: Cross Jamie , Nguyen Bao H. , Zhang Bo 
Published: October 2022

AbstractIn this paper, we provide direct evidence for an increasingly critical role of China in the world oil markets. Specifically, our empirical results confirm that the influence of China's oil demand on the oil price of the world has increased over time and surpassed that of the United States. The contribution of demand perspective from China are formally quantified by three key variables of world oil market, which are world oil production, real economic activity and the real price of crude oil. The impact of oil demand from China is used to compared with those from other oil markets, including shocks from the United States. We also construct suitable proxies that reflect real economic activity from these economies, and identify them via a structural vector autoregressive model to disentangle the influence of oil demand from China, the United States and the rest of the world. Our results confirm the finding of recent research, which is the increasing demand for oil from developing countries has become a key driver of the price of oil.
Construction of α‐Fe2O3/g‐C3N4/COF Ternary Hybrid with Double Z‐Scheme Heterojunctions for Photocatalysis**
Authors: Lu Wenjie , Qin Tong , Wang Bowei , Li Jiayi , Zhu Hongqi , Bai Guoyi , Li Yang , Chen Ligong , Yan Xilong 
Published: October 2022

AbstractBy report, Z‐scheme heterojunction can markedly improve the photocatalytic performance of semiconductor materials. Thus, we rationally designed and successfully prepared α‐Fe2O3/g‐C3N4/covalent organic framework (COF) ternary photocatalyst with double Z‐scheme heterojunctions. XRD, FT‐IR, and TEM characterized results demonstrated its successful construction. Further characterization results revealed that the existing double Z‐scheme heterojunctions endow the material with superior photoelectric properties and realize the synergy among the three components, thus achieving the excellent separation of photo‐generated carriers. Consequently, it exhibited excellent performance in the photocatalytic degradation of tetracycline (TC). Therefore, the double Z‐scheme heterojunction strategy proposed in this study established a promising approach for constructing high‐efficiency photocatalysts.
Functional Assays Evaluating Immunosuppression Mediated by Myeloid‐Derived Suppressor Cells
Authors: Reuven Or , Mikula Ivan , Ashkenazi‐Preiser Hadas , Twaik Nira , Ben‐Meir Kerem , Meirow Yaron , Daniel Leonor , Kariv Guy , Kurd Mahdi , Baniyash Michal 
Published: October 2022

AbstractMyeloid‐derived suppressor cells (MDSCs) are heterogenous populations of immature myeloid cells that can be divided into two main subpopulations, polymorphonuclear (PMN) MDSCs and monocytic (M) MDSCs. These cells accumulate during chronic inflammation, characterizing an array of pathologies such as cancer, inflammatory bowel disease, and infectious and autoimmune diseases, and induce immunosuppression. The suppressive effects of MDSCs on the immune system are studied mainly when focusing on their features, functions, and impact on target cells such as T cells, natural killer cells, and B cells, among others. Herein, we describe methods for the analysis of MDSC immunosuppressive features and functions, measuring different mediators that contribute to their activities and how they impact on T cell function. The protocols described are a continuation to those in a companion Current Protocols article by Reuven et al. (2022), which uses a generated single‐cell suspension and isolated cells to test their activity. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.This article was corrected on 13 December 2024. See the end of the full text for details.Basic Protocol 1: Evaluating MDSC suppressive featuresAlternate Protocol 1: Dichlorofluorescein diacetate–based reactive oxygen species detectionSupport Protocol 1: Detection of nitric oxide secretionSupport Protocol 2: Measurement of arginase activityBasic Protocol 2: Evaluating MDSC suppressive functionAlternate Protocol 2: In vitro effects of MDSCs on expression of T cell receptor complex during activationSupport Protocol 3: Effect of MDSCs on interferon γ productionBasic Protocol 3: Effect of MDSCs on T cell proliferationBasic Protocol 4: Effect of MDSCs on T cell cytotoxic activityAlternate Protocol 3: In vivo cytotoxicity assayBasic Protocol 5: Analysis of MDSC differentiation
Development of the reciprocating airflow generator for wave power generator and study on the vertical axis turbine characteristics in reciprocating airflow
Authors: Tsuji Kentaro , Shiono Mitsuhiro 
Published: October 2022

AbstractRenewable energy is attracting attention as an alternative to current mainstream power generator sources. One source of renewable energy is wave energy. We devised a simulator for the OWC‐type wave power generator and developed a reciprocating airflow generator is generated reciprocating airflow by using the reciprocating motion of a piston. This piston motion was achieved by converting the rotational motion of the motor into linear motion of the ball screw via gears. Furthermore, we used a vertical axis turbine with arc camber blades and studied on the turbine characteristics. Owing to the reciprocation of the piston, a substantially sinusoidal reciprocating airflow was generated in the measurement section. It was confirmed that a reciprocating airflow speed was generated for all the target values. A load characteristic test of the turbine was conducted. It was confirmed that the maximum turbine output was 1.60 W.
Maternal Transfer and Apical and Physiological Effects of Dietary Hexabromocyclododecane Exposure in Parental Fathead Minnows (Pimephales promelas)
Authors: Malala Irugal Bandaralage Susari , Ignacio Bertucci Juan , Park Brad , Green Derek , Brinkmann Markus , Masse Anita , Crump Doug , Basu Niladri , Hogan Natacha , Hecker Markus 
Published: October 2022

Abstract
Hexabromocyclododecane (HBCD) is a persistent organic pollutant that has been characterized as an endocrine disruptor, undergoes maternal transfer, and hinders development and growth in oviparous organisms. The present study examined the apical effects of dietary HBCD (11.5, 36.4, 106 mg/kg, wet wt) on adult fathead minnow exposed for 49 days and the subsequent accumulation and maternal transfer kinetics in adult tissue and eggs, respectively. Exposed adults displayed a significant increase in egg production in the medium treatment group, but no other significant effects were noted. Maternal transfer of dietary HBCD had a similar egg-to-muscle ratios (EMR) in the low and medium treatment groups (1.65 and 1.27 [wet wt], respectively). However, the high treatment group deviated from other treatments with an EMR of 4.2 (wet wt), potentially due to differences in total lipid content in food and/or reaching diffusion/lipid saturation limits in adult tissue, resulting in lower accumulation in the adult muscle tissue. A positive correlation was observed between egg HBCD concentration and time of exposure, which indicates that maternal transfer of HBCD is of concern in fish, and further studies should be conducted to fully elucidate the potential adverse effects that may be observed in the early life stage of oviparous organisms. Environ Toxicol Chem 2023;42:143–153. © 2022 SETAC
The influence of oil price uncertainty on stock liquidity
Authors: Zhang Qin , Wong Jin Boon 
Published: October 2022

AbstractUsing high‐frequency intraday data, this study provides strong empirical evidence that elevated oil price uncertainty has a significant and negative influence on stock liquidity. More specifically, the results suggest that large oil‐related corporations are most affected, followed by small‐listed firms more generally. Further analysis reveals that liquidity providers widen the bid‐ask spreads to protect themselves during periods of high oil price uncertainty for large‐listed firms, particularly those in the oil industry. These findings are robust to various measures of oil price uncertainty, different market conditions, structural break analysis and show the influence of oil price movements extends to stock liquidity.