bluuebunny/crossref_metadata_2025_split huggingface dataset:
KMT2D mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumor-induced regulatory T cell trafficking via FBXW7-NOTCH-MYC/TGF-β1 axis
Authors: Liu Qing-Xiao , Zhu Yue , Yi Hong-Mei , Shen Yi-Ge , Wang Li , Cheng Shu , Xu Peng-Peng , Xu Hai-Min , Zhou Lu-Ting , Huang Yao-Hui , Huang Chuan-Xin , Fu Di , Ji Meng-Meng , Wang Chao-Fu , Zhao Wei-Li 
Published: April 2022

Abstract
Background Histone methyltransferase KMT2D is one of the most frequently mutated genes in diffuse large B-cell lymphoma (DLBCL) and has been identified as an important pathogenic factor and prognostic marker. However, the biological relevance of KMT2D mutations on tumor microenvironment remains to be determined.Methods KMT2D mutations were assessed by whole-genome/exome sequencing (WGS/WES) in 334 patients and by targeted sequencing in 427 patients with newly diagnosed DLBCL. Their correlation with clinical outcomes and immune cell characteristics were evaluated. Underlying molecular mechanisms on tumor microenvironment were investigated both in vitro and in vivo.Results Among all 761 DLBCL patients, somatic mutations in KMT2D were observed in 143 (18.79%) patients and significantly associated with advanced Ann Arbor stage and MYC expression ≥ 40%, as well as inferior progression-free survival and overall survival. In B-lymphoma cells, the mutation or knockdown of KMT2D inhibited methylation of lysine 4 on histone H3 (H3K4), downregulated FBXW7 expression, activated NOTCH signaling pathway and downstream MYC/TGF-β1, resulting in alterations of tumor-induced regulatory T cell trafficking. In B-lymphoma murine models established with subcutaneous injection of SU-DHL-4 cells, xenografted tumors bearing KMT2D mutation presented lower H3K4 methylation, higher regulatory T cell recruitment, thereby provoking rapid tumor growth compared with wild-type KMT2D via FBXW7-NOTCH-MYC/TGF-β1 axis. Conclusions Our work thus contributed to the better understanding of aberrant histone methylation on tumor microenvironment as an alternative mechanism of tumor progression in DLBCL.
Antimalarial potential of Gymnema inodorum leaf extract and dihydroartemisinin combination against Plasmodium berghei infected mice
Authors: Somsak Voravuth , Ounjaijean Sakaewan 
Published: April 2022

Abstract
Chemotherapy plays a crucial role in malaria control. However, the main obstacle to treatment has been the rise of parasite resistance to most antimalarial drugs. Artemisinin-based combination therapies (ACTs) remain today’s most effective antimalarial therapies. However, malaria parasite tolerance to ACTs is now increasingly prevalent, especially in Southeast Asia. Consequently, this creates the need for effective alternative antimalarials. Interestingly, the effectiveness of Gymnema inodorum leaf extract (GIE) as an antimalarial candidate was reported earlier. Therefore, this study sought to evaluate the antimalarial potential of dihydroartemisinin (DHA) based combination therapy with GIE against Plasmodium berghei in a mouse model. Drug assessment was carried out on P. berghei ANKA (PbANKA) using the standard 4-day test for determination of fifty percent effective dose (ED50) of DHA and GIE individually. Moreover, the combination of DHA and GIE was also evaluated based on fixed-ratio strategy, 100/0, 80/20, 60/40, 40/60, 20/80, and 0/100 of ED50 of DHA and GIE, respectively. Overall, 2 mg/kg and 100 mg/kg of DHA and GIE resulted in ED50 against PbANKA. In combination, the ratio of 60/40 (DHA/GIE) had a considerable the highest antimalarial activity significantly (p < 0.001) against PbANKA with 88.95% inhibition that indicated a synergism efficacy (CI value = 0.68695). Additionally, this ratio was protected PbANKA infected mice from BW loss and PCV reduction with prolonged survival time throughout the 30 day follow up. This study points to better efficacy of GIE as an alternative drug partner in combination to enhance antimalarial efficacy of DHA against PbANKA infected mice.
Rubber–Ice friction
Authors: Persson Bo , Tada T. , Kawasaki S. , Shimizu R. , Persson B.N.J. 
Published: April 2022

Abstract
We study the friction when a rectangular tire tread rubber block is slid on an ice surface at different temperatures ranging from −38○C to −2○C, and sliding speeds ranging from 3 µm/s to 1 cm/s. At low temperatures and low sliding speeds we propose that an important contribution to the friction force is due to slip between the ice surface and ice fragments attached to the rubber surface. At temperatures above −10○C or for high enough sliding speeds a thin premelted water film occur on the ice surface and the contribution to the friction from shearing the area of real contact is small. In this case the dominant contribution to the friction force comes from viscoelastic deformations of the rubber by the ice asperities.
Evolution of work ability, quality of life and self-rated health in a police department after remodelling shift schedule
Authors: Velasco-Garrido Marcial , Herold Robert , Rohwer Elisabeth , Mache Stefanie , Terschüren Claudia , Preisser Alexandra M. , Harth Volker 
Published: April 2022

Abstract
Background: There exists a great diversity of schedules concerning the way shift work is organized and implemented with ample agreement regarding recommendable features of a shift system. In order to adapt the shift schedule of a metropolitan police department to current recommendations, a remodelled shift schedule was introduced in 2015. The aim of this study was to evaluate the potential associations between the remodelled shift schedule and work ability, quality of life and self-rated health after one and five years.Methods: A controlled before-and- after study were conducted during the piloting phase (2015-2016) as well as a 5-year follow-up using paper questionnaires. Outcome parameters included work ability, quality of life and self-rated health.Results: Work ability, quality of life and self-rated health improved after the first year of the newly implemented shift schedule among police officers working in the piloting police stations compared to those working according to the former schedule. In 5-year follow-up differences between indicators diminished.Conclusions: The implementation of a remodelled shift schedule including more 12-hours shifts accompanied by more days off and a coherent weekend off duty was not associated with detrimental effects to work ability, quality of life or self-reported health among police officers.
Non-invasive transdermal delivery of biomacromolecules with fluorocarbon-modified chitosan for melanoma immunotherapy and transdermal vaccines
Authors: Zhu Wenjun , Wei Ting , Jin Qiutong , Lu Jiaqi , Xu Jun , Chen Muchao , Zhu Jiafei , Chao Yu , Chen Qian , Liu Zhuang 
Published: April 2022

Abstract
Transdermal drug delivery has been regarded as an alternative to oral delivery and subcutaneous injection for its non-invasiveness, improved patient compliance and avoidance of the first-pass effect. However, needleless transdermal delivery of biomacromolecules remains a challenge. Herein, a transdermal delivery platform is developed to achieve highly efficient non-invasive transdermal delivery of biomacromolecules. In this system, fluorocarbon modified chitosan (FCS) is optimized as an effective yet biocompatible transdermal carrier to assemble with different proteins including immune checkpoint blockade (ICB) antibodies, and antigens such as the spike (S) protein of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The formed FCS-containing nanocomplexes exhibited rather effective transdermal penetration ability via both intercellular and transappendageal routes. Interestingly, non-invasive transdermal delivery of ICB antibodies by FCS induced stronger immune responses to treat mouse melanoma compared to intravenous injection of free antibodies, while presenting reduced systemic toxicity. Moreover, transdermal delivery of SARS-CoV-2 vaccine using FCS-containing nanocomplexes resulted in comparable humoral immunity as well as improved cell immunity and immune memory compared to that achieved with subcutaneous vaccine injection. Thus, FCS-based transdermal delivery systems may provide a compelling opportunity to overcome the skin barrier for efficient transdermal delivery of biomacromolecules, widening the range of therapeutics that can be topically administered.
Contribution of histone acetylation to the serotonin-mediated long-term synaptic plasticity in terrestrial snails
Authors: Zuzina Alena B. , Balaban Pavel M. 
Published: April 2022

Abstract
Serotonin plays a decisive role in long-term synaptic plasticity and long-term memory in mollusks. Previously, we demonstrated that histone acetylation is a critical regulatory mechanism of long-term memory in terrestrial snail. At the behavioral level, many studies were done in Helix to elucidate the role of histone acetylation and serotonin. However, the impact of histone acetylation on long-term potentiation of synaptic efficiency in electrophysiological studies in Helix has been studied only in one paper. Here we investigated effects of serotonin, histone deacetylases inhibitors sodium butyrate and trichostatin A, and a serotonergic receptor inhibitor methiothepin on long-term potentiation of synaptic responses in vitro. We demonstrated that long-term potentiation was suppressed by methiothepin but rescued by co-application of histone deacetylase inhibitors sodium butyrate or trichostatin A. Increased histone acetylation compensated the loss of serotonergic activation. We showed that single serotonin application in combination with histone deacetylase blockade could mimic the effect of repeated serotonin applications and be enough for sustained long-lasting synaptic changes. The data obtained demonstrated that histone deacetylases blockade ameliorated deficits in synaptic plasticity induced by different paradigms (methiothepin treatment, the weak training protocol with single application of serotonin), suggesting that histone acetylation contributes to the serotonin-mediated synaptic plasticity.
Enhanced stent visualization system for percutaneous coronary intervention in patients with chronic kidney disease: effects on contrast media volume and radiation exposure
Authors: Tian Yunpeng , Zhao Xiangdong , Yang Yang , Cai Xiaoshu , Jian Lian , Guo Suzhen , Xia Dasheng , Chen Xin , Li Chao , Guo Qianyu , Chen Bingwei , Lu Chengzhi 
Published: April 2022

Abstract
Objective: We aimed to assess the impact of using enhanced stent visualization (ESV) systems on contrast media volume and radiation dose in percutaneous coronary intervention (PCI), especially for patients with chronic kidney disease (CKD). Background: Coronary heart disease (CHD) is associated with chronic kidney disease (CKD) as they share the similar pathological pathway. In addition, the iodinated contrast media used for angiography is a risk factor for contrast-associated acute kidney injury (CA-AKI), which could aggravate the progression of CKD. We hypothesized that ESV systems have the potential to reduce the use of contrast media as well as radiation dose, however, few study reported the impact on contrast media with use of ESV systems. Methods: We retrospectively collected 124 patients with acute coronary syndromes underwent PCI from May 2020 to July 2021. Patients were divided into ESV-guided group (n = 64) and angiography-guided group (n = 60). Procedural parameters including contrast media volume, radiation exposure (in Air Kerma), number of cines, cine frames, fluoroscopy and procedure time were recorded and analyzed.Results: Groups were comparable for patient characteristics. A significant reduction in contrast media volume (179.41 ± 16.76 ml vs. 114.5 ± 13.56ml, p = 0.0001), radiation exposure (693.99 ± 351.04 mGy vs. 1439.45 ± 973.01 mGy, p = 0.001) and procedure time (47.75 ± 15.43 min vs. 65.88 ± 23.81 min, p = 0.029) with the use of ESV systems. Similar results were observed in the subgroup analysis for the patients with CKD.Conclusion: This study suggested that the use of ESV is associated with reduced contrast media usage, radiation dose and procedure time during PCI. The same results were observed in subgroup analysis in patients with CKD, which is of great significance in decreasing the occurrence of contrast-induced nephropathy and mitigating the progression of CKD and CHD.
Evaluation of the effectiveness of androgenetic alopecia treatment in men us- ing platelet-rich plasma: A systematic review of randomized, placebo-con- trolled trials
Authors: Borowiecka Julia Maria , Pałka Łukasz 
Published: April 2022

Abstract
Platelet-Rich Plasma becomes more and more popular treatment alternative or additional method in androgen alopecia (AGA). AGA is a multifactorial disease, in which testosterone plays a significant role in influencing hair growth. The aim was to evaluate the effectiveness of PRP treatment in androgenetic alopecia affecting men. The research was performed using the following databases: PubMed, Embase, and Cochrane Library. The effects were measured with a Tricho-Scan and the measure of the effect was the difference between the initial and final hair density. A significant difference was observed between the areas of the scalp where PRP injections were made and those where saline was administered. Compared to conventional minoxidil 5% topical platelet-rich plasma therapy PRP is more effective in male-pattern baldness treatment. A beneficial effect of combining PRP Therapy with minoxidil 5% was observed, therefore PRP is not only an excellent alternative for patients in whom the minoxidil 5% topical monotherapy did not bring the expected effect or experienced unacceptable side effects, but also can be used as a complementary therapy.
Ecological scheme of cyanobacterial responses to hydrogen peroxide and antioxidant enzymes against diurnal light intensity
Authors: Asaeda Takashi , Rahman Mizanur , Matsuo Akihiko , Abeynayaka H. Damitha L. 
Published: April 2022

Abstract
Diurnal variations of the oxidative stress condition of cyanobacteria were measured by field observations and laboratory experiments. In the field experiment, on clear summer days, transparent bottles, filled with surface water, were set up near the water surface, and several depths to 1 m deep. One bottle from each depth was collected every three hours together with the measurement of the photosynthetically active radiation (PAR). In the laboratory experiment, two cyanobacterial species were exposed to gradually increasing then decreasing light intensities. The samples were analyzed with the hydrogen peroxide (H2O2) concentration, the catalase activities (CAT), protein and chlorophyll a (Chl-a) contents. Regardless of difference in cyanobacterial species, similar diurnal trends were obtained. Protein contents did not change for one day experiments. Biologically generated H2O2 increased following the PAR intensity variation, with the delay of a few hours. The H2O2 concentration per protein was given as an increasing function of the exposure period (T) to the PAR. The amount of Chl-a content declined with the H2O2 per protein value. The model was developed to estimate the H2O2 per protein value, elucidating that the effect of the cyanobacterial biomass and the surface layer mixing on the oxidative stress and mortality.
Springer Nature 2021 L A T E X template ARAM: a Technology Acceptance Model to Ascertain the Behavioural Intention to Use Augmented Reality
Authors: Marto Anabela , Gonçalves Alexandrino , Melo Miguel , Bessa Maximino , Silva Rui 
Published: April 2022

Abstract
The expansion of augmented reality across society, its availability in mobile platforms and the novelty character it embodies by appearing in a growing number of areas have raised new questions related to people’s predisposition to use this technology in their daily life. Acceptance models, which have been updated in accordance with technological breakthroughs and society changes, are known to be great tools for predicting the intention to use a technological system. This paper proposes a new acceptance model aiming to ascertain the intention to use augmented reality technology in heritage sites-the Augmented Reality Acceptance Model (ARAM). ARAM relies on the use of the UTAUT model’s constructs to which the new and adapted constructs of Trust Expectancy, Technological Innovation, Computer Anxiety and Hedonic Motivation are added. This model was validated with data gathered from 528 participants. Results confirm ARAM as a reliable tool to determine the acceptance of augmented reality technology. The direct impact of Performance Expectancy, Facilitating Conditions and Hedonic Motivation is validated as having a positive influence on Behavioural Intention. Trust Expectancy and 1 Springer Nature 2021 L A T E X template ARAM: a Technology Acceptance Model Technological Innovation are demonstrated to have a positive influence on Performance Expectancy whereas Hedonic Motivation is negatively influenced by Effort Expectancy and by Computer Anxiety. The research, thus, supports ARAM as a suitable model to ascertain the behavioural intention to use augmented reality in new areas of activity.
Medical Image Reconstruction Method Based on Smooth L0 Norm
Authors: Xiang Jianhong , Xiang Hao , Wang Linyu , Zhong Yu 
Published: April 2022

Abstract
Compressed sensing (CS) has a wide range of applications in sparse signal reconstruction. Aiming at the problems of low recovery accuracy and long reconstruction time of existing reconstruction algorithms in medical imaging, this paper proposes a corrected smoothing L0 algorithm based on compressed sensing (CSL0). Firstly, an approximate hyperbolic tangent function (AHTF) which is more similar to the L0 norm is proposed to approximate the L0 norm. Secondly, aiming at the problem that the steepest descent method has a “sawtooth phenomenon” and the modified Newton method is sensitive to the initial value selection, the steepest descent method and the modified Newton method are jointly optimized to improve the reconstruction accuracy. In addition, the CCSL0 algorithm is proposed by introducing a composite inverse proportional function, and the reconstruction time of the CSL0 algorithm is further optimized. Finally, the CSL0 algorithm and CCSL0 algorithm are simulated on medical images. The results show that the proposed algorithm improves the reconstruction accuracy of the test image by 0 – 0.96 dB.
Combining ability studies in muskmelon (Cucumis melo L.) for quantitative and qualitative traits
Authors: Duradundi Shivaji kallappa , Gasti V. D. , Mulge Ravindra , Masuthi Deelipkumar A. 
Published: April 2022

Abstract
An investigation was undertaken to study the combining ability in muskmelon (Cucumis melo L.) for quantitative and qualitative traits, during 2016–2017 at department of vegetable science, K. R. C. College of Horticulture, Arabhavi. Combining ability analysis was conducted to understand the nature of gene action of quantitative traits and to identify promising parents for breeding Programme in muskmelon. The ten lines and three testers were sown and crossed in a line X tester mating system to obtain 30 F1 hybrid combinations. Among the lines KM-1, KM-2 and KM-10 were the best general combiner for fruit yield per hectare and most of the quantitative traits. The crosses like KM-2 x PS, KM-1 x DK and KM-3 x PS exhibited significant SCA effects for fruit yield per hectare and most of the other traits.
Bisphenol S Promotes the Progression of Prostate Cancer by Regulating the Expression of COL1A1 and COL1A2.
Authors: Liu shengdi , Li Hua 
Published: April 2022

Abstract
In recent decades, Bisphenol S (BPS), which have been considered as alternatives for Bisphenol A (BPA), have become widely used in personal care products, paper products and food. Clarifying the relationship between bisphenol and tumors is of great significance for the treatment and prevention of diseases. In this work, we discovered a new method to predict the correlation between bisphenol interactive genes and tumors. The transcriptome profile and interactive genes of bisphenol were obtained from the Cancer Genome Atlas and Genotype-Tissue Expression, Comparative Toxicology Genomics and PharmMapper database. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis showed that interactive genes were mainly enriched in prostate cancer. Gene targetd prediction and gene set variation analysis proved that bisphenol exert potential effects on prostate cancer. The operating characteristic curves and survival analysis showed that role of COL1A1 and COL1A2 in predicting the prognosis of prostate cancer. Cell counting kit-8 assay demonstrated that the cells with BPS-treated could remarkably promote the cell proliferation ability in both PC-3 and LNCap cells. Wound healing assay and the transwell assay demonstrated that the cells with BPS-treated could significantly promote the cell invasion capacity of prostate cells. Two key genes, COL1A1 and COL1A2, were significantly upregulated with BPS-treated in the PC-3 and LNCap cells.
PROJETO T.I.P.O. ASSIM! - PROMOÇÃO DE SAÚDE E PROTAGONISMO COM ADOLESCENTES
Authors: REINHEIMER DE BITTENCOURT ELISANGELA , De Camargo Machado Magale 
Published: April 2022

O estudo versa sobre o Projeto T.I.P.O. Assim! (Territorialidade, intersetorialidade, promoção de saúde, protagonismo e ocupação de espaços) - desenvolvido em bairros de periferia de uma cidade do estado do Rio Grande do Sul (RS), Brasil. Partiu-se da interrogação sobre as possibilidades de acesso aos adolescentes em situação de vulnerabilidade e uso de álcool e outras drogas associados ao sofrimento psíquico, que não aderem a abordagens de cuidado ao nível ambulatorial de convencionais de saúde mental. Objetivou-se promover saúde e protagonismo como alternativa ao consumo de álcool e outras drogas; possibilitar a subjetivação de adolescentes; criar estratégias de acesso, cuidado e prevenção em saúde mental de adolescentes. O referencial teórico foi a psicanálise, o conceito de sublimação foi relacionado à transformação da pulsão em produção cultural. Traz-se a territorialidade, associada ao princípio do Sistema Único de Saúde (SUS). A pesquisa-intervenção foi proposta na metodologia. O projeto iniciou com o estudo das demandas nos territórios; formação de seis grupos com encontros semanais, com atividades ligadas à música, à poesia, à culinária e à percepção sensorial. Realizou-se um questionário com quarenta adolescentes para auxiliar na análise dos resultados. A exemplo de respostas, encontram-se: 97,5% dos adolescentes se reconhecem como protagonistas, 55% chegaram ao projeto pelo convite de amigos, demonstrando a importância da multiplicação; 57,5% perceberam mudanças nas relações de amizade, 37,5% na escola e 25% na família. A construção de espaços coletivos com adolescentes, a utilização de atividades culturais favorecem o protagonismo, a subjetivação e promovem saúde e prevenção ao uso de álcool e outras drogas.
POTENSI BUDIDAYA TANAMAN SONOKELING MELALUI PEMBERDAYAAN KELOMPOK BM CREATIVE WOODS DI DESA GIRIHARJO, KECAMATAN PANGGANG, KABUPATEN GUNUNG KIDUL
Authors: Safitri Kinanti Indah 
Published: April 2022

The object of this research in Giriharjo Village which has potential trees that can be used for making wooden crafts. The kind of tree is sonokeling. Sonokeling has a strong texture with brownish purple fibers. Sonokeling wood is high quality wood and it used for home decor, wooden watch, flashdisk cover and various other products. This research was carried out on since October 2018 – Mei 2019. The research method used qualitative- study case approach. The data collection technique through in depth interview, observation and documentation . The research result showed that in Giriharjo Village , there are BM creative woods group that make wooden crafts. However, this group has a problem to continue production process because the human resources have a lack of interest and constraints in marketing strategy. Therefore, innovation is needed that includes: institutional strengthening, cultivation sonokeling plants and internalization effective marketing strategy. Keywords: Empowerment, Institution, Cultivation, Marketing.