bluuebunny/crossref_metadata_2025_split huggingface dataset:
POLLEN WALL ABORTION 1 is essential for pollen wall development in rice
Authors: Zhang Lisha , Liu Yang , Wei Gang , Lei Ting , Wu Jingwen , Zheng Lintao , Ma Honglei , He Guanghua , Wang Nan 
Published: September 2022

Abstract
The integrity of pollen wall structures is essential for pollen development and maturity in rice (Oryza sativa L.). In this study, we isolated and characterized the rice male-sterile mutant pollen wall abortion 1 (pwa1), which exhibits a defective pollen wall (DPW) structure and has sterile pollen. Map-based cloning, genetic complementation, and gene knockout experiments revealed that PWA1 corresponds to the gene LOC_Os01g55094 encoding a coiled-coil domain-containing protein. PWA1 localized to the nucleus, and PWA1 was expressed in the tapetum and microspores. PWA1 interacted with the transcription factor TAPETUM DEGENERATION RETARDATION (TDR)-INTERACTING PROTEIN2 (TIP2, also named bHLH142) in vivo and in vitro. The tip2-1 mutant, which we obtained by clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9-mediated gene editing, showed delayed tapetum degradation, sterile pollen, and DPWs. We determined that TIP2/bHLH142 regulates PWA1 expression by binding to its promoter. Analysis of the phenotype of the tip2-1 pwa1 double mutant indicated that TIP2/bHLH142 functions upstream of PWA1. Further studies suggested that PWA1 has transcriptional activation activity and participates in pollen intine development through the β-glucosidase Os12BGlu38. Therefore, we identified a sterility factor, PWA1, and uncovered a regulatory network underlying the formation of the pollen wall and mature pollen in rice.
Sensory Profiles in Patients with Low Back Pain with and Without Radiculopathy
Authors: Reimer Maren , Witthöft Johanna , Greinacher Jessica , Sachau Juliane , Forstenpointner Julia , Hüllemann Philipp , Binder Andreas , Gierthmühlen Janne , Baron Ralf 
Published: September 2022

AbstractObjectiveDuring routine clinical evaluation, it can be challenging to differentiate between lumbar radiculopathy (RAD) and lower back pain with non-radicular somatic referred pain (SRP) or even axial non-radiating low back pain (LBP). The aim of this study was to characterize patients with RAD, axial LBP (aLBP), and SRP on the basis of somatosensory profiles.MethodsPatients with LBP (n = 54) were assessed with quantitative sensory testing in the area of LBP and, in cases of RAD, additionally in the area of projecting pain. Questionnaires (PainDETECT®, EuroQol-5D, Medical Outcomes Study Sleep Scale, Hannover Functional Ability Questionnaire for Back Pain, Roland Morris Disability Questionnaire, Short Form-12 Health Survey, and Hospital Anxiety and Depression Scale) were answered by all patients.ResultsPatients with RAD (n = 12) had higher pain intensity scores (numeric rating scale: 5.7 ± 1.5 vs 4.1 ± 2.2; P &lt; 0.05) and higher PainDETECT scores (14.6 ± 6.13 vs 9.7 ± 6.2; P &lt; 0.05) than did patients with aLBP and SRP (n = 42). Patients with RAD had a more pronounced loss of small-fiber function, increased mechanical hyperalgesia, and a trend toward increased sensitivity to thermal pain in the area of LBP compared with patients with aLBP and SRP. Within patients with RAD, sensory profiles of the area of projecting pain and the area of LBP did not differ. Pressure pain hyperalgesia (measured by pressure pain threshold) and loss of mechanical detection (measured by mechanical detection threshold) in combination with the PainDETECT items numbness and prickling reached the best predictive value in detecting a radiculopathy.ConclusionsPatients with RAD demonstrated more somatosensory abnormalities than did patients with aLBP and SRP, including increased mechanical hyperalgesia and a loss of mechanical detection. The combination of pressure pain threshold, mechanical detection threshold, numbness, and prickling in the area of LBP can be a time-efficient tool to identify patients with RAD.
The efficacy and safety of one-stage endoscopy combined with intrarenal surgery (mini-nephrostomy tract) in the prone split-leg position for complex renal calculi
Authors: Yu Kai , Du Zhebin , Xuan Hanqing , Cao Yang , Zhong Hai , Li Fangzhou , Chen Qi 
Published: September 2022

Abstract

Background
The goal of this study was to determine the safety and efficacy of endoscopic combined intrarenal surgery (ECIRS) performed in the prone split-leg position for the treatment of complex renal stones.

Materials and methods
A mature ECIRS protocol was designed. Retrospective analysis was conducted of medical records between January 2020 and December 2021 of patients with complex renal stones at one center who underwent ECIRS by 2 skilled surgeons using retrograde flexible ureteroscopy and mini-percutaneous nephrolithotomy in the prone split-leg position.

Results
A total of 44 patients were included in this study. Mean stone size was 26.1 ± 12.7 mm, and the number of calyces involved was 4.36 ± 2.09. Mean operative time was 71.1 ± 21.8 minutes. Postoperative decline in hemoglobin was 15.8 ± 9.8 g/L. Seventy-five percent of patients achieved stone-free status. The mean number of residual stones was 2.8 ± 2.3, and the mean residual stone size was 10.30 ± 4.76 mm. Six patients (13.6%) developed postoperative complications, including 4 with fever during the first 2 days postoperatively and 2 patients with transient postoperative pain. No patients developed severe complications.

Conclusions
Endoscopic combined intrarenal surgery in the prone split-leg position can be performed safely by experienced surgeons using retrograde flexible ureteroscopy in conjunction with mini-percutaneous nephrolithotomy as a successful technique for the treatment of complex renal stones.
A comprehensive visual report of urodynamic study in rats with spinal cord injury
Authors: Salehi-Pourmehr Hanieh , Mahmoudi Javad , Saeedi Vahdat Arman , Hajebrahimi Sakineh , Abolhasanpour Nasrin 
Published: September 2022

Abstract

Objectives
Spinal cord injury (SCI) is one of the most debilitating and expensive traumatic conditions. Chronic complications after SCI have a particularly negative impact on patients’ functional independence and quality of life. Urodynamic study (UDS) provides a quantitative assessment of lower urinary tract function in these patients. In many fields, animal models are considered a precursor to clinical trials, so research using laboratory animals play a major role in knowledge acquisition.

Materials and methods
Twelve female Wistar rats (13 weeks old, 220–270 g) were divided randomly into 2 groups: sham or SCI. The sham-operated group underwent a laminectomy at T9–T10 without any spinal cord damage, while the SCI group underwent a complete transection at the T9–T10 vertebral level. We performed cystometry in all animals at the end of the fourth week. In this article, we visualize all procedures for catheter implementation and UDS in animals for the first time at Tabriz University of Medical Sciences, Iran, using a locally developed animal UDS device.

Results
The UDS results showed that the bladders in the SCI group were overactive and that peak and baseline pressures increased significantly in rats with SCI when compared with the sham group (p < 0.05 for all). Conversely, significant reductions in bladder compliance and intercontraction interval were observed in the SCI group (p < 0.05 for both).

Conclusions
This comprehensive visual report will be very useful to all researchers in the field of urology. Furthermore, the measurable variables of the UDS device have been described in this study.
Predictors of stone-free rate after a single-session extracorporeal shockwave lithotripsy for a single kidney stone measuring 10 to 20 mm: A private center experience
Authors: Faddan Amr A. , Najieb Osama , Gadelkareem Rabea A. 
Published: September 2022

Abstract

Background
Despite the continuous update of guidelines for the management of kidney stones, the ability to predict a successful response to extracorporeal shockwave lithotripsy (SWL) remains a topic of research. This is due to the need to refine the decision making in the context of technological advancements and current pandemics. This study aimed to determine the predictors of stone-free rate (SFR) after single-session SWL in adult patients with a single kidney stone 10 to 20 mm in diameter.

Materials and methods
A retrospective review of the records of patients with a single kidney stone 10 to 20 mm in diameter was performed at a private SWL center from December 2019 to February 2021. Univariate and multivariate analyses were performed for stone- and patient-related factors, using the Student t and Mann-Whitney U tests for the quantitative variables and the Fisher exact and Pearson correlation tests for the qualitative variables.

Results
A total of 138 patients were eligible for this study, including 92 men and 46 women. The mean age was 38.6 ± 12.4 years, and the mean body mass index (BMI) was 25.9 ± 3.4 kg/m2. Four weeks after SWL, 120 patients (87%) were free of stones, and 18 (13%) needed further treatment. Univariate analyses showed that SFR was negatively correlated with increased BMI (p = 0.0001), maximum stone length (p = 0.0001), transverse diameter of the stone (p = 0.0001), number of shocks per session (p = 0.052), and Hounsfield unit (HU) (p = 0.0001). Multivariate analysis revealed that HU (p = 0.009), maximum stone length (p = 0.01), BMI (p = 0.000), and presence of double-J stent (p = 0.034) were independent risk factors for failure of single-session SWL in the treatment of kidney stones 10 to 20 mm in diameter. The estimated average cost per case was USD 450.5.

Conclusions
Increased HU, maximum stone length, BMI, and presence of double-J stents were independent risk factors for low SFR after single-session SWL for a kidney stone 10 to 20 mm in diameter. The cost of SWL remains an advantage in the private sector.
From the challenge of assessing autonomy to the instruments used in practice: A scoping review
Authors: Lima Andreia Maria Novo , da Silva Martins Maria Manuela Ferreira , Ferreira Maria Salomé Martins , Fernandes Carla Sílvia , Schoeller Soraia Dornelles , Parola Vítor Sérgio Oliveira 
Published: September 2022

Antecedents:
Autonomy is one of the essential components to live a quality life. Monitoring this autonomy is, in effect, essential, to allow the nurses to conceive, implement and evaluate interventions aimed at its promotion or even maintenance. For this reason, this scoping review aims to map the evidence to identify and analyze the instruments used to assess the person’s autonomy, which emerges from scientific production.

Methods:
Scoping review based on the recommended principles by the Joanna Briggs Institute. The research was realized in the databases: Scopus (excluding MEDLINE), CINAHL complete (via EBSCO, Excluding MEDLINE), and MEDLINE (via PubMed). Two independent reviewers evaluated the articles’ pertinence for the study’s investigation, the extraction, and synthesis of articles.

Results:
After the analysis, according to the inclusion criteria established, 34 articles were selected, allude to 7 different instruments to assess autonomy.

Conclusions:
The need for further development at this level is highlighted, namely through the construction and validation of more comprehensive instruments, integrating the different components of the concept of autonomy.
Portuguese adaptation and validation of a patient-reported experience measure for patients with rheumatoid arthritis: A protocol study
Authors: Oliveira Daniela , Bernardes Miguel , Vaz Carlos , Costa Lúcia , Fonseca João Almeida , Jácome Cristina 
Published: September 2022

Abstract

Background:
There is a growing attention to patient-reported experience measures in assessing the quality of care in patient-centered care models. A specific patient-reported experience measure for patients with rheumatoid arthritis (RA) has been developed in the United Kingdom—Commissioning for Quality in Rheumatoid Arthritis Patient-Reported Experience Measure. This patient-reported experience measure might be feasible to be used in Portugal, yet an adaptation and validation process is needed. Therefore, the aims of this study will be to translate and cross-culturally adapt the Portuguese version of the Commissioning for Quality in Rheumatoid Arthritis Patient-Reported Experience Measure, evaluate its content and face validity through a qualitative approach, and evaluate its psychometric properties through a clinical field testing.

Methods:
This study is based on a multimethod approach combining qualitative and quantitative approaches. This study will include patients with RA from a single rheumatology center. Three sequential phases are planned: Commissioning for Quality in Rheumatoid Arthritis Patient-Reported Experience Measure translation and cultural adaptation, Commissioning for Quality in Rheumatoid Arthritis Patient-Reported Experience Measure content and face validity assessed through 2 focus groups with at least 10 patients, and the Commissioning for Quality in Rheumatoid Arthritis Patient-Reported Experience Measure field testing through a cross-sectional study with 50 patients.

Conclusions:
By involving patients with RA in the validation and implementation of the Commissioning for Quality in Rheumatoid Arthritis Patient-Reported Experience Measure, we expect to demonstrate the usefulness of this specific patient-reported experience measure to improve health care provided to patients with RA.
Understanding the Mental Health Impacts of the COVID-19 Pandemic on Railway Workers
Authors: Cogan Nicola , McGibbon Megan , Gardiner Amy , Morton Liza 
Published: September 2022

ObjectiveRailway workers have provided an essential service throughout the COVID-19 pandemic. This study explored the effects of COVID-19 on the mental well-being of railway workers (N = 906) in the United Kingdom during the third lockdown period.MethodThe online survey included measures of COVID-19–related risk factors (perceived risk, stress, burnout, trauma) and protective factors (resilience coping, team resilience, general help seeking) associated with mental well-being. Responses were analyzed using multiple regression and content analysis.ResultsCOVID-19–related risk factors negatively predicted well-being. Higher scores on adaptive resilience, intentions to seek help, and team resilience significantly predicted higher mental well-being scores. Mental health decline throughout the COVID-19 pandemic and concerns for the future were reported.ConclusionsBuilding a resilient railway workforce requires attention to staff mental well-being and to ensuring that support systems are robust and accessible.
Improving the Hearing Status Discrimination of the Health Utilities Index, Mark 3: Design of the Hearing Status Classification System
Authors: Dixon Peter R. , Feeny David , Tomlinson George , Cushing Sharon L. , Chen Joseph M. 
Published: September 2022

Objective
Redesign the health status classification system of the Health Utilities Index, Mark 3 (HUI-3) “hearing” attribute to improve its discrimination of hearing-impaired health states.

Study Design
Domain and item selection from a previously generated item set guided by a domain and item importance survey, structural independence, and cognitive interviews with patients.

Setting
Tertiary referral center.

Participants
Patients with a range of hearing loss severities, etiologies, and treatment experiences participated in the domain and item importance survey (n = 108) and hour-long cognitive interviews (n = 10).

Main Outcome and Measure(s)
Subattributes and levels for the novel Hearing attribute. Domain and item importance was scored on a seven-point Likert scale (1, not at all important; 7, extremely important).

Results
Mean domain importance was highest for “speech in noise” (6.21; 95% confidence interval, 5.98–6.43) and lowest for “benefits of hearing loss” (3.46; 95% confidence interval, 3.03–3.89). Domains with moderate or greater (r ≥ 0.5) domain importance Pearson correlation or construct overlap that interfered with structural independence were combined into single subattributes. Iterative adjustments to instructions, items, and phrasing were guided by cognitive interviews to derive the final instrument with seven subattributes: speech, environmental sounds, localization, listening effort, tinnitus, music, and assistive devices. The novel hearing attribute defines 25,920 unique hearing states.

Conclusion
The novel HUI-hearing is part of a comprehensive health utility instrument designed for individuals with hearing loss. Pending derivation of a hearing single attribute utility function and evaluation of measurement properties, our innovative approach could be used to improve health utility measurement for impairments described by any of the other HUI-3 attributes.
Influence of Sociodemographic and Occupational Factors on Safety Culture in Neonatal Intensive Care in Brazil
Authors: Manzo Bruna Figueiredo , Correa Allana dos Reis , Chaves Taylane Vilela , Damaceno Maria Alice Fonseca , Antunes Karine , Macieira Tamara G. R. , Diniz Catharine Galvão , Parker Leslie A. 
Published: September 2022

Background:
Patient safety culture is influenced by factors such as professional category, experience, and age. Understanding these factors can inform initiatives to improve safety.

Purpose:
To evaluate the relationship between sociodemographic and occupational characteristics on health professionals' perception of patient safety culture.

Methods:
A cross-sectional study involving 514 health care professionals from Brazilian neonatal intensive care units was conducted using the Hospital Survey on Patient Safety Culture.

Results:
Several sociodemographic and occupational characteristics were associated with higher perceptions of safety culture, including older age and having a higher level of education.

Conclusion:
Sociodemographic and occupational factors may influence the safety culture in neonatal intensive care units and should be considered when developing and implementing strategies to improve safety.
Dory Flop Sign in Monkeypox: 2 Cases
Authors: Peñuelas Leal Rodrigo , Labrandero Hoyos Carolina , Grau Echevarría Andrés , Martínez Domenech Álvaro , Lorca Sprohnle Javier , Casanova Esquembre Andrés , Magdaleno Tapial Jorge , Hernández Bel Pablo 
Published: September 2022

Abstract
We describe 2 monkeypox cases in human immunodeficiency virus–positive men who have sex with men with undetectable viral loads. Both patients presented with the dory flop sign.
An evaluation of digital partner notification tool engagement & impact for patients diagnosed with gonorrhea and syphilis
Authors: Folke Tomas , Menon-Johansson Anatole Sebastian 
Published: September 2022

Abstract

Objective
Partner Notification (PN) is a key public health service to alert the partners of patients diagnosed with a sexually transmitted infection and then support these individuals to test. PN is a challenge to deliver because of the personal nature of this communication and the time it requires to deliver effectively. Digital tools have been developed to support patients to inform their partners; unfortunately, it has not been possible to measure engagement with – and the impact of – these solutions. A digital PN tool (dPNt) was developed for use by the healthcare provider to (1) deliver anonymous PN, (2) support the partner to locate and test at an appropriate local service, and (3) track real-time outcome metrics. To assess the usefulness of this tool we analysed dPNt adoption and performance in the delivery of PN services.

Method
dPNt engagement for 5,715 patients diagnosed by 23 healthcare providers with either gonorrhea or syphilis in 2019 was analysed to determine what factors were associated with the use of this tool. The PN performance and a sub analysis of the patients who used dPNt was compared with the adjusted aggregate PN performance reported by Public Health England (PHE).

Results
Overall dPNt engagement was 21% and 27% for patients with gonorrhea and syphilis, respectively. Male gendered patients with gonorrhea and younger patients with either diagnosis were more likely to engage with dPNt. Non-engagement with dPNt was associated with a significantly higher number of partners reported as already seen and tested at the time that PN was initiated. The overall number of partners seen and tested per index patient increased in the clinics using dPNt from 0.43 to 0.84 for gonorrhea and 0.71 to 0.94 for syphilis, relative to PHE baseline results. Half of all prospective partner testing verification was done automatically by dPNt and no interaction was demonstrated between dPNt and standard methods.

Conclusion
dPNt engagement increased when more partners needed to be informed of their risk of infection. Future work is planned to improve the use of and engagement with dPNt to alert and test more partners or their risk and improve public health.
Comparing Sexual Network Mean Active Degree Measurement Metrics among Men who have Sex with Men
Authors: Chandra Christina , Morris Martina , Van Meter Connor , Goodreau Steven M. , Sanchez Travis , Janulis Patrick , Birkett Michelle , Jenness Samuel M. 
Published: September 2022

ABSTRACT

Background
Mean active degree is an important proxy measure of cross-sectional network connectivity commonly used in HIV/STI epidemiology research. No current studies have compared measurement methods of mean degree using a cross-sectional study design for men who have sex with men (MSM) in the United States. We compared mean degree estimates based on reported ongoing main and casual sexual partnerships (current method) against dates of first and last sex (retrospective method).

Methods
We used data from ARTnet, a cross-sectional survey of MSM in the U.S. (2017–2019). ARTnet collected data on the number and types of sexual partners in the past year, limited to the 5 most recent partners (data truncation). We quantified partnerships for months 0–12 prior to the survey date (retrospective method), and compared that to ongoing partnerships on the day of survey (current method). We used linear regression to understand the impact of truncated partnership data on mean degree estimation.

Results
The retrospective method yielded similar degree estimates to the current for months proximate to the day of survey. The retrospective method mean degree systematically decreased as the month increased from 0–12 months prior to survey date. This was driven by data truncation: among participants with >5 partners in the past year compared to those with ≤5, the average change in main partnership degree between 12 and 0 months prior to survey date was -0.05 (95% CI: -0.08, -0.03) after adjusting for race/ethnicity, age, and education. The adjusted average change in casual partnership degree was -0.40 (95% CI: -0.45, -0.35).

Conclusions
The retrospective method underestimates mean degree for MSM in surveys with truncated partnership data, especially for casual partnerships. The current method is less prone to bias from partner truncation when the target population has high rate of partners per year.