bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics of monocyte-derived macrophage diversity in experimental myocardial infarction
Authors: Rizzo Giuseppe , Gropper Julius , Piollet Marie , Vafadarnejad Ehsan , Rizakou Anna , Bandi Sourish Reddy , Arampatzi Panagiota , Krammer Tobias , DiFabion Nina , Dietrich Oliver , Arias-Loza Anahi-Paula , Prinz Marco , Mack Matthias , Schlepckow Kai , Haass Christian , Silvestre Jean-Sébastien , Zernecke Alma , Saliba Antoine-Emmanuel , Cochain Clément 
Published: August 2022

Abstract

Aims
Macrophages have a critical and dual role in post-ischaemic cardiac repair, as they can foster both tissue healing and damage. Multiple subsets of tissue resident and monocyte-derived macrophages coexist in the infarcted heart, but their precise identity, temporal dynamics, and the mechanisms regulating their acquisition of discrete states are not fully understood. To address this, we used multi-modal single-cell immune profiling, combined with targeted cell depletion and macrophage fate mapping, to precisely map monocyte/macrophage transitions after experimental myocardial infarction.

Methods and results
We performed single-cell transcriptomic and cell-surface marker profiling of circulating and cardiac immune cells in mice challenged with acute myocardial infarction, and integrated single-cell transcriptomes obtained before and at 1, 3, 5, 7, and 11 days after infarction. Using complementary strategies of CCR2+ monocyte depletion and fate mapping of tissue resident macrophages, we determined the origin of cardiac macrophage populations. The macrophage landscape of the infarcted heart was dominated by monocyte-derived cells comprising two pro-inflammatory populations defined as Isg15hi and MHCII+Il1b+, alongside non-inflammatory Trem2hi cells. Trem2hi macrophages were observed in the ischaemic area, but not in the remote viable myocardium, and comprised two subpopulations sequentially populating the heart defined as Trem2hiSpp1hi monocyte-to-macrophage intermediates, and fully differentiated Trem2hiGdf15hi macrophages. Cardiac Trem2hi macrophages showed similarities to ‘lipid-associated macrophages’ found in mouse models of metabolic diseases and were observed in the human heart, indicating conserved features of this macrophage state across diseases and species. Ischaemic injury induced a shift of circulating Ly6Chi monocytes towards a Chil3hi state with granulocyte-like features, but the acquisition of the Trem2hi macrophage signature occurred in the ischaemic tissue. In vitro, macrophages acquired features of the Trem2hi signature following apoptotic-cell efferocytosis.

Conclusion
Our work provides a comprehensive map of monocyte/macrophage transitions in the ischaemic heart, constituting a valuable resource for further investigating how these cells may be harnessed and modulated to promote post-ischaemic heart repair.
The Pictorial Fit-Frail Scale—Malay version (PFFS-M): reliability and validity testing in Malaysian primary care
Authors: Ahip Sally S , Ghazali Sazlina S , Theou Olga , Samad Azah A , Lukas Sabrina , Mustapha Ummu K , Thompson Mark Q , Visvanathan Renuka 
Published: August 2022

AbstractBackgroundThis study investigated the reliability and convergent validity of the PFFS-Malay version (PFFS-M) among patients (with varying educational levels), caregivers, and health care professionals (HCPs). PFFS-M cutoffs for frailty severity were developed.MethodsThis is a cross-sectional study from 4 primary care clinics where 240 patients aged &gt;60 years and their caregivers were enrolled. Patients were assigned to a nurse or a health care assistant (HCA) for 2 separate PFFS-M assessments administered by HCPs of the same profession, as well as by a doctor during the first visit (inter-rater reliability). Patients were also administered the Self-Assessed Report of Personal Capacity & Healthy Ageing (SEARCH) tool, a 40-item frailty index, by a research officer. The correlation between patients’ PFFS-M scores and SEARCH tool scores determined convergent validity. Patients returned 1 week later for PFFS-M reassessment by the same HCPs (test–retest reliability). Caregivers completed the PFFS-M for the patient at both clinic visits. Classification cut-points for the PFFS-M were derived against frailty categories defined through the SEARCH tool.ResultsThe inter-rater (intraclass correlation coefficient [ICC] = 0.92 [95% CI, 0.90–0.93)] and test–retest (ICC = 0.94 [95% CI, 0.92–0.95]) reliability between all raters was excellent, including by patients’ education levels. The convergent validity was moderate (r = 0.637, p &lt; 0.001), including for varying educational background. PFFS-M categories were identified as: 0–3, no frailty; 4–5, at risk of frailty; 6–8, mild frailty; 9–12, moderate frailty; and &gt;13, severe frailty.ConclusionPFFS-M is a reliable and valid tool with frailty severity scores now established for use of this tool in primary care clinics.
TOP-2 is differentially required for the proper maintenance of the cohesin subunit REC-8 on meiotic chromosomes in Caenorhabditis elegans spermatogenesis and oogenesis
Authors: Rourke Christine , Jaramillo-Lambert Aimee 
Published: August 2022

Abstract
During meiotic prophase I, accurate segregation of homologous chromosomes requires the establishment of chromosomes with a meiosis-specific architecture. The sister chromatid cohesin complex and the enzyme Topoisomerase II (TOP-2) are important components of meiotic chromosome architecture, but the relationship of these proteins in the context of meiotic chromosome segregation is poorly defined. Here, we analyzed the role of TOP-2 in the timely release of the sister chromatid cohesin subunit REC-8 during spermatogenesis and oogenesis of Caenorhabditis elegans. We show that there is a different requirement for TOP-2 in meiosis of spermatogenesis and oogenesis. The loss-of-function mutation top-2(it7) results in premature REC-8 removal in spermatogenesis, but not oogenesis. This correlates with a failure to maintain the HORMA-domain proteins HTP-1 and HTP-2 (HTP-1/2) on chromosome axes at diakinesis and mislocalization of the downstream components that control REC-8 release including Aurora B kinase. In oogenesis, top-2(it7) causes a delay in the localization of Aurora B to oocyte chromosomes but can be rescued through premature activation of the maturation promoting factor via knockdown of the inhibitor kinase WEE-1.3. The delay in Aurora B localization is associated with an increase in the length of diakinesis bivalents and wee-1.3 RNAi mediated rescue of Aurora B localization in top-2(it7) is associated with a decrease in diakinesis bivalent length. Our results imply that the sex-specific effects of TOP-2 on REC-8 release are due to differences in the temporal regulation of meiosis and chromosome structure in late prophase I in spermatogenesis and oogenesis.
Aging in Nigeria: A Growing Population of Older Adults Requires the Implementation of National Aging Policies
Authors: Mbam Kingsley Chima , Halvorsen Cal J , Okoye Uzoma Odera 
Published: August 2022

Abstract
While the continent of Africa currently has the lowest share of adults aged 60 and older in its population among all world regions, the total number of older Africans is projected to triple between 2020 and 2050. Nigeria—Africa’s leading economy and most populated country—has the highest number of older people in the continent and the 19th highest across the globe, with the population of Nigerians aged 65 and older projected to nearly triple by 2050. However, the increase in older Nigerians is occurring against the backdrop of extreme poverty, unsolved development problems, socioeconomic inequality, the HIV/AIDS epidemic, and a decline in the traditional care and support of older adults. Additionally, the absence of an operational national aging policy or safety net services and programs poses a unique challenge to older Nigerians and their families. In this article, we examined social statistics and the current literature to describe aging in the Nigerian context. Our findings highlight the importance of establishing effective governmental policy interventions for the adequate care and support of older Nigerians and the training of gerontological professionals. This article will describe the demography of aging in Nigeria, significant areas of research, key scholars and publicly available data sets, public policy issues, and emerging issues affecting the health and well-being of older Nigerians.
Soy protein concentrate replacing animal protein supplements and its impacts on intestinal immune status, intestinal oxidative stress status, nutrient digestibility, mucosa-associated microbiota, and growth performance of nursery pigs
Authors: Deng Zixiao , Duarte Marcos Elias , Jang Ki Beom , Kim Sung Woo 
Published: August 2022

Abstract
This study was to evaluate the effects of soy protein concentrate (SPC) supplementation replacing animal protein supplements on intestinal immune status, intestinal oxidative stress status, nutrient digestibility, mucosa-associated microbiota, and growth performance of nursery pigs. Thirty-two newly weaned pigs at 21 d of age with 6.4 ± 0.4 kg body weight (BW) were allotted to four treatments in a randomized complete block design with initial BW and sex as blocks. Pigs were fed for 35 d in three phases. Dietary treatments were SPC 0% (diets with fish meal 4/2/1%, poultry meal 10/8/4%, blood plasma 4/2/1%, and crude protein 24.6/22.6/20.9% for phase 1/2/3, respectively), SPC 33%, SPC 66%, and SPC 100% (SPC 0% diets with SPC replacing 33/66/100% of animal protein supplements, respectively). Pigs were euthanized on day 35 to collect jejunal mucosa and tissues to evaluate intestinal immune status, intestinal oxidative stress status, intestinal morphology, and mucosa-associated microbiota in the jejunum. Titanium dioxide was added in phase three diets as an indigestible marker. Ileal digesta was collected to measure apparent ileal digestibility (AID) of nutrients. Data were analyzed using MIXED and NLMIXED procedures of SAS. Increasing SPC supplementation by replacing animal protein supplements linearly decreased (P &lt; 0.05) the BW, ADG, and ADFI of pigs during the overall period, and linearly increased (P &lt; 0.05) peptide tyrosine tyrosine (PYY) in jejunum. Increasing SPC supplementation linearly decreased (P &lt; 0.05) feed cost per weight gain. In the exponential model, SPC can replace animal protein supplements up to 10.5% and 16.5% without reducing the ADG and ADFI of pigs, respectively. The SPC 100% decreased (P &lt; 0.05) Helicobacteraceae, Campylobacteraceae, alpha diversity, and changed beta diversity of microbiota in the jejunal mucosa. In conclusion, SPC supplementation replacing animal protein supplements reduced growth performance by reducing feed intake, which might be related to increased PYY. However, 10.5% and 16.8% of animal protein supplements can be replaced by SPC without affecting BW gain and feed intake of nursery pigs, respectively. Complete removal of animal protein supplements by SPC supplementation modulated the composition of jejunal mucosa-associated microbiota by reducing Helicobacteraceae and Campylobacteraceae, whereas without affecting the intestinal immune status, intestinal oxidative stress status, intestinal morphology, and AID of nutrients in nursery pigs.
Alteration of Vesicle-Associated Membrane Protein-Binding Protein B in α-Synuclein Aggregates in Lewy Body Disease
Authors: Mori Fumiaki , Nakamura Yukino , Miki Yasuo , Tanji Kunikazu , Kon Tomoya , Tomiyama Masahiko , Kakita Akiyoshi , Wakabayashi Koichi 
Published: August 2022

Abstract
α-Synuclein (α-Syn) binds to vesicle-associated membrane protein-binding protein B (VAPB) in the endoplasmic reticulum membrane. Recent studies have shown that α-Syn-immunoreactive Lewy pathology is characterized by membrane crowding, including vesicular structures. To elucidate the role of VAPB and vesicular structures in Parkinson’s disease (PD) and in dementia with Lewy bodies (DLB), the relationships among VAPB, vesicular structures, and Lewy pathology were investigated by immunohistochemistry and immunoelectron microscopy in 8 PD and 4 DLB autopsy cases. The proportions of VAPB-negative neurons in the substantia nigra in PD and in the temporal cortex in DLB were significantly higher than those in 5 controls. In PD, the incidence of α-Syn inclusions in VAPB-negative neurons was significantly higher (77.4%) than in VAPB-positive neurons (1.6%) in the substantia nigra. In DLB, the incidence of α-Syn inclusions in VAPB-negative neurons was also significantly higher (65.3%) than in VAPB-positive neurons (2.8%) in the temporal cortex. Immunoelectron microscopy revealed that α-Syn and VAPB were localized to filamentous structures of Lewy bodies (LBs). However, only a few vesicular structures labeled with anti-α-Syn were observed within LBs. These findings suggest that reduction of VAPB is involved in the disease processes of PD and DLB, although vesicular structures may not directly contribute to the formation of LBs.
Different evolutionary pathways to generate plant fructan exohydrolases
Authors: Van den Ende Wim 
Published: August 2022

This article comments on:Oku S, Ueno K, Sawazaki Y, Maeda T, Jitsuyama Y, Suzuki T, Onodera S, Fujino K, Shimura H. 2022. Functional characterization and vacuolar localization of fructan exohydrolase derived from onion (Allium cepa). Journal of Experimental Botany 73,4908–4922.
Effects of probiotic supplementation on performance of resistance and aerobic exercises: a systematic review
Authors: de Paiva Anne K F , de Oliveira Erick P , Mancini Laura , Paoli Antonio , Mota João F 
Published: August 2022

Abstract

Context
Strenuous exercise may lead to negative acute physiological effects that can impair athletic performance. Some recent studies suggest that probiotic supplementation can curtail these effects by reducing the permeability of the intestinal barrier, yet results are inconsistent.

Objective
The aim of this systematic review is to assess the effects of probiotic supplementation on athletic performance.

Data Sources
The PubMed/MEDLINE, Cochrane, and Scopus databases were searched for articles that assessed the effects of probiotic supplementation on athletic performance.

Data Extraction This systematic review is reported according to
PRISMA guidelines. Risk of bias was assessed through the Cochrane RoB 2.0 tool. Seventeen randomized clinical trials assessing athletic performance as the primary outcome were included. In total, 496 individuals (73% male) comprising athletes, recreationally trained individuals, and untrained healthy individuals aged 18 to 40 years were investigated.

Data Analysis
Three studies showed an increase or an attenuation of aerobic performance (decline in time to exhaustion on the treadmill) after supplementation with probiotics, while 3 found an increase in strength. However, most studies (n = 11) showed no effect of probiotic consumption on aerobic performance (n = 9) or muscular strength (n = 2). The most frequently used strain was Lactobacillus acidophilus, used in 2 studies that observed positive results on performance. Studies that used Lactobacillus plantarum TK10 and Lactobacillus plantarum PS128 also demonstrated positive effects on aerobic performance and strength, but they had high risk of bias, which implies low confidence about the actual effect of treatment.

Conclusion
There is not enough evidence to support the hypothesis that probiotics can improve performance in resistance and aerobic exercises. Further well-controlled studies are warranted.
Social investment as a conceptual framework for analysing well-being returns and reforms in 21st century welfare states
Authors: Hemerijck Anton , Ronchi Stefano , Plavgo Ilze 
Published: August 2022

AbstractWelfare provision is often conceived through the lens of decommodification and analysed in (re)distributive terms. This article argues that a distributive approach does not sufficiently capture the complexity of 21st century welfare state dynamics. It proposes re-conceptualizing provision as a mix of three policy functions: raising and maintaining human capital stock; easing the flow of gendered life-course and labour-market transitions; guaranteeing social safety-net buffers. This analytical perspective allows theorizing life-course multiplier effects and policy (non-)complementarities, both at the level of individual objective and subjective well-being and in terms of aggregate employment, poverty and fiscal sustainability. This perspective also enables us to extend the temporal horizon of welfare politics beyond short-term electoral logics for explaining welfare reform. The article underscores how methodological pluralism remains key for understanding contemporary welfare states, and for grasping welfare outcomes and institutional change in a research endeavour that involves both generalization and contextualization.
Sedation versus General Anesthesia for Tracheal Intubation in Children with Difficult Airways: A Cohort Study from the Pediatric Difficult Intubation Registry
Authors: Sequera-Ramos Luis , Laverriere Elizabeth K. , Garcia-Marcinkiewicz Annery G. , Zhang Bingqing , Kovatsis Pete G. , Fiadjoe John E. ,   for the PeDI Collaborative
Published: August 2022

Background
Sedated and awake tracheal intubation approaches are considered safest in adults with difficult airways, but little is known about the outcomes of sedated intubations in children. The primary aim of this study was to compare the first-attempt success rate of tracheal intubation during sedated tracheal intubation versus tracheal intubation under general anesthesia. The hypothesis was that sedated intubation would be associated with a lower first-attempt success rate and more complications than general anesthesia.

Methods
This study used data from an international observational registry, the Pediatric Difficult Intubation Registry, which prospectively collects data about tracheal intubation in children with difficult airways. The use of sedation versus general anesthesia for tracheal intubation were compared. The primary outcome was the first-attempt success of tracheal intubation. Secondary outcomes included the number of intubation attempts and nonsevere and severe complications. Propensity score matching was used with a matching ratio up to 1:15 to reduce bias due to measured confounders.

Results
Between 2017 and 2020, 34 hospitals submitted 1,839 anticipated difficult airway cases that met inclusion criteria for the study. Of these, 75 patients received sedation, and 1,764 patients received general anesthesia. Propensity score matching resulted in 58 patients in the sedation group and 522 patients in the general anesthesia group. The rate of first-attempt success of tracheal intubation was 28 of 58 (48.3%) in the sedation group and 250 of 522 (47.9%) in the general anesthesia group (odds ratio, 1.06; 95% CI, 0.60 to 1.87; P = 0.846). The median number of intubations attempts was 2 (interquartile range, 1 to 3) in the sedation group and 2 (interquartile range, 1, 2) in the general anesthesia group. The general anesthesia group had 6 of 522 (1.1%) intubation failures versus 0 of 58 in the sedation group. However, 16 of 58 (27.6%) sedation cases had to be converted to general anesthesia for successful tracheal intubation. Complications were similar between the groups, and the rate of severe complications was low.

Conclusions
Sedation and general anesthesia had a similar rate of first-attempt success of tracheal intubation in children with difficult airways; however, 27.6% of the sedation cases needed to be converted to general anesthesia to complete tracheal intubation. Complications overall were similar between the groups, and the rate of severe complications was low.

Editor’s Perspective

What We Already Know about This Topic

What This Article Tells Us That Is New
PROMIS Utilization in Pediatric Orthopaedics: A Scoping Review
Authors: Eisenberg Matthew T. , Block Andrew M. , Ganapathy Aravinda K. , Huckleby Jeremy M. , Nepple Jeffrey J. 
Published: August 2022

Background:
The lack of a common patient-reported outcome metric used among the orthopaedic population is a problem that has been previously identified by the American Academy of Orthopaedic Surgeons. The Patient-Reported Outcomes Measurement Information System (PROMIS) was developed by the National Institute of Health with the goal of creating a precise and efficient measurement tool for patient-reported symptoms, functioning, and health-related quality of life to be used in clinical research. A study summarizing its use in the pediatric orthopaedic population has not been previously performed.

Methods:
We performed a literature search of Ovid Medline, Embase, Scopus, and the Cochrane Central Register of Controlled Trials from 2010 to August 2021. There were 1961 unique citations included after the removal of 1756 duplicates. After initial screening, 183 studies were screened under full-text review leaving a final number of 51 studies included in this scoping review.

Results:
Pediatric PROMIS studies were grouped by body part or sub-speciality: “Hand and Upper Extremity” (25.5%, n=13), “Sports” (23.5%, n=12), “Spine” (13.7%, n=7), “Trauma” (13.7%, n=7), “General Pediatric Orthopaedics” (11.8%, n=6), “Lower Extremity” (9.8%, n=5), and “Orthopaedic Oncology” (2%, n=1). An increase in studies utilizing PROMIS was seen throughout the study period with only 3 studies published from 2013 to 2016 to 39 in 2020 and 2021 alone. The 3 most frequently used pediatric PROMIS domains were Pain Interference (76.5%, n=39/51), Mobility (60.8%, n=31/51), and Upper Extremity (54.9%, n=28/51). 64.3% (n=9/14) of the included studies which reported on the floor effects of Pain Interference exhibited a significant floor effect. In all, 77.8% (n=7/9) of the included studies which reported on ceiling effects of Upper Extremity exhibited a significant ceiling effect.

Conclusion:
The use of PROMIS increased significantly since the first publication in 2013 suggesting orthopaedic providers have increasingly utilized PROMIS in their day-to-day practice as an outcome measure. Ceiling and floor effects were prominent in several of the included domains (Pain Interference and Upper Extremity). Overall, PROMIS measures are efficient, reliable, and effective to use.

Level of Evidence:
IV.