bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Transition to Siblinghood: An Understudied Yet Influential Process Pertinent to Social Work Research
Authors: Ward Kaitlin P 
Published: January 2022

Abstract
Across cultures, the majority of individuals will have a sibling relationship in their lifetime. Of particular importance to parents and children is the transition to siblinghood, which refers to the process wherein a child becomes a sibling. Although many parents are concerned about how their children will adjust during the transition to siblinghood, scant empirical work on this subject exists. Available empirical work on the transition to siblinghood has stemmed from developmental psychology; however, social work researchers have yet to deeply explore this body of work. The transition to siblinghood is a topic that is rife with opportunity for social work researchers, who have the ability to study this transition from culturally sensitive, person-in-environment, and social justice perspectives. This article provides an overview of the elements and importance of the transition to siblinghood, introduces a theoretical framework relevant to social workers, reviews existent empirical work on the transition to siblinghood, and elucidates limitations in the literature that social work researchers are adept to address. The article concludes with four recommendations to researchers who are interested in studying the transition to siblinghood through a social work lens.
The effects of course format, sex, semester, and institution on student performance in an undergraduate animal science course
Authors: Vinyard James R , Peñagaricano Francisco , Faciola Antonio P 
Published: January 2022

Abstract
The transition of courses from in-person to an online format due to the COVID-19 pandemic could have potentially affected overall student performance in lecture-based courses. The objective of this case study was to determine the impact of course format, as well as the effects of student sex, time of year at which the course was taken, and the institution it was taken at on student performance in an undergraduate animal science course. The course used for this study was taught at two institutions (University of Florida; UF and University of Nevada, Reno; UNR) over 7 yr (2014–2017 at UNR and 2018–2021 at UF). Student’s performance (n = 911) was evaluated using both quizzes and exams from 2014 through the spring semester 2020 and only exams were used for summer and fall semesters of 2020 and the spring and summer semesters of 2021. The final score (out of 100%) for each student was used to evaluate student’s performance. In addition, students were classified as high-performing students, if they scored ≥95% and low-performing students, if they scored ≤70%. The variables evaluated were the effects of semester (spring, summer, or fall), institution (UF or UNR), sex (male or female), number of teaching assistants (TAs; 0–13), and course format (online or in-person). The course was taught in-person at UNR and in-person and online at UF. The spring semester of 2020 was taught in-person until March but was switched to online approximately 9 wk after the semester started and was considered an online semester for this analysis. As the course was only taught online at UF, the variable course format was assessed using UF records only. Data were analyzed using both linear models and logistic regressions. The probability that students were high performing was not affected by sex or institution. Interestingly, both fall semester and the online format had a positive, desirable effect on the probability that students were high performing. The probability that students were low performing was not affected by sex. However, if a student performed poorly in the class, they were more likely to have taken the course at UNR, or at UF with many TAs. Thus, student’s performance was impacted by changing the course format, as well as institution, the number of TAs, and the semester in which the course was taken.
Osteology and phylogenetic relationships ofLigabuesaurus leanzai(Dinosauria: Sauropoda) from the Early Cretaceous of the Neuquén Basin, Patagonia, Argentina
Authors: Bellardini Flavio , Coria Rodolfo A , Pino Diego A , Windholz Guillermo J , Baiano Mattia A , Martinelli Augustin G 
Published: January 2022

AbstractOsteological knowledge of the sauropod dinosaur Ligabuesaurus leanzai is increased by the description of new postcranial elements assigned to the holotype MCF-PVPH-233. Furthermore, a newly referred specimen, MCF-PVPH-228, is recognized after a detailed revision of the abundant sauropod material collected from the Lohan Cura Formation outcrops in the Cerro de los Leones locality (southern Neuquén Basin, Patagonia, Argentina). Recent laboratory preparation and fieldwork allowed us to recognize several new morphological features of the pectoral and pelvic girdles and the cervical and caudal anatomy. Thus, a new diagnosis of Ligabuesaurus is proposed that includes new autapomorphies and a unique combination of features. A phylogenetic analysis based on this new material recovers Ligabuesaurus as a non-titanosaurian somphospondylan, more derived than Sauroposeidon. Therefore, we discuss the palaeobiogeographical implications for the diversification and distribution of South American somphospondylans, especially in the Neuquén Basin, which are closely related to the early stages of evolution of Titanosauria. In this context, Ligabuesaurus represents one of the more complete Early Cretaceous Titanosauriformes and the earliest non-titanosaurian somphospondylan of South America. Finally, the new information on Ligabuesaurus contributes not only to reconstruction of the sauropod faunal composition of south-western Gondwana, but also sheds light on the early stages and emergence of titanosaurians.
The multiple molecular dimensions of long noncoding RNAs that regulate gene expression and tumorigenesis
Authors: Marcia Marco 
Published: January 2022

Purpose of review
LncRNAs are emerging as key regulators of gene expression and they ensure homeostasis during cell differentiation and development, replication, and adaptation to the environment. Because of their key central role in regulating the biology of living cells, it is crucial to characterize how lncRNAs function at the genetic, transcriptomic, and mechanistic level.

Recent findings
The low endogenous abundance and high molecular complexity of lncRNAs pose unique challenges for their characterization but new methodological advances in biochemistry, biophysics and cell biology have recently made it possible to characterize an increasing number of these transcripts, including oncogenic and tumor suppressor lncRNAs. These recent studies specifically address important issues that had remained controversial, such as the selectivity of lncRNA mechanisms of action, the functional importance of lncRNA sequences, secondary and tertiary structures, and the specificity of lncRNA interactions with proteins.

Summary
These recent achievements, coupled to population-wide medical and genomic approaches that connect lncRNAs with human diseases and to recent advances in RNA-targeted drug development, open unprecedented new perspectives for exploiting lncRNAs as pharmacological targets or biomarkers to monitor and cure cancer, in addition to metabolic, developmental and cardiovascular diseases.
Transcriptional and epigenetic control of early life cell fate decisions
Authors: Al-Mousawi Jasmina , Boskovic Ana 
Published: January 2022

Purpose of review
Global epigenetic reprogramming of the parental genomes after fertilization ensures the establishment of genome organization permissive for cell specialization and differentiation during development. In this review, we highlight selected, well-characterized relationships between epigenetic factors and transcriptional cell fate regulators during the initial stages of mouse development.

Recent findings
Blastomeres of the mouse embryo are characterized by atypical and dynamic histone modification arrangements, noncoding RNAs and DNA methylation profiles. Moreover, asymmetries in epigenomic patterning between embryonic cells arise as early as the first cleavage, with potentially instructive roles during the first lineage allocations in the mouse embryo. Although it is widely appreciated that transcription factors and developmental signaling pathways play a crucial role in cell fate specification at the onset of development, it is increasingly clear that their function is tightly connected to the underlying epigenetic status of the embryonic cells in which they act.

Summary
Findings on the interplay between genetic, epigenetic and environmental factors during reprogramming and differentiation in the embryo are crucial for understanding the molecular underpinnings of disease processes, particularly tumorigenesis, which is characterized by global epigenetic rewiring and progressive loss of cellular identity.
Autophagy, not apoptosis, plays a role in lumen formation of eccrine gland organoids
Authors: Du Lijie , Zhang Lei , Zhao Junhong , Chen Zixiu , Liu Xiang , Cao Manxiu , You Lei , Zhang Yonghong , Fu Xiaobing , Li Haihong 
Published: January 2022

Abstract

Background:
Sweat secreted by eccrine sweat glands is transported to the skin surface through the lumen. The eccrine sweat gland develops from the initial solid bud to the final gland structure with a lumen, but how the lumen is formed and the mechanism of lumen formation have not yet been fully elucidated. This study aimed to investigate the mechanism of lumen formation of eccrine gland organoids (EGOs).

Methods:
Human eccrine sweat glands were isolated from the skin for tissue culture, and the primary cultured cells were collected and cultured in Matrigel for 14 days in vitro. EGOs at different development days were collected for hematoxylin and eosin (H&E) staining to observe morphological changes and for immunofluorescence staining of proliferation marker Ki67, cellular motility marker filamentous actin (F-actin), and autophagy marker LC3B. Western blotting was used to detect the expression of Ki67, F-actin, and LC3B. Moreover, apoptosis was detected using a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis assay kit, and the expression of poly (ADP-ribose) polymerase and Caspase-3 was detected by Western blot. In addition, 3-methyladenine (3MA) was used as an autophagy inhibitor to detect whether the formation of sweat glands can be effectively inhibited.

Results:
The results showed that a single gland cell proliferated rapidly and formed EGOs on day 4. The earliest lumen formation was observed on day 6. From day 8 to day 14, the rate of lumen formation in EGOs increased significantly. The immunofluorescence and Western blot analyses showed that the expression of Ki67 gradually decreased with the increase in days, while the F-actin expression level did not change. Notably, the expression of autophagy marker LC3B was detected in the interior cells of EGOs as the apoptosis signal of EGOs was negative. Compared with the control group, the autophagy inhibitor 3MA can effectively limit the formation rate of the lumen and reduce the inner diameter of EGOs.

Conclusion:
Using our model of eccrine gland 3D-reconstruction in Matrigel, we determined that autophagy rather than apoptosis plays a role in the lumen formation of EGOs.
General Anxiety Is Associated with Problematic Initial Recovery After Carpal Tunnel Release
Authors: Ryan Claire , Miner Harrison , Ramachandran Shyam , Ring David , Fatehi Amirreza 
Published: January 2022

Abstract

Background
Carpal tunnel release can stop the progression of idiopathic median neuropathy at the wrist (carpal tunnel syndrome). Intermittent symptoms tend to resolve after surgery, but loss of sensibility can be permanent. Both pathophysiology (severe neuropathy) and mental health (symptoms of despair or worry) contribute to problematic recovery after carpal tunnel release, but their relative associations are unclear.

Question/purpose
Is problematic initial recovery after carpal tunnel release associated with psychologic distress rather than with disease severity?

Methods
We retrospectively studied 156 patients who underwent in-office carpal tunnel release between November 2017 and February 2020, and we recorded their symptoms of anxiety (Generalized Anxiety Disorder-7 [GAD]) and depression (Patient Health Questionnaire), signs of severe median neuropathy (loss of sensibility, thenar muscle atrophy, and palmar abduction weakness), and problematic recovery. The initial recovery (first 2 weeks) was categorized as problematic if the patient was upset about persistent numbness, experienced unsettling postoperative pain, developed hand stiffness, or experienced wound issues—all of which are routinely recorded in the medical record by the treating surgeon along with signs of severe median neuropathy. Twenty-four percent (38 of 156) of patients had a problematic initial recovery characterized by distress regarding persistent numbness (16% [25 of 156]), unsettling pain (8% [12 of 156]), hand stiffness (5% [8 of 156]), or wound issues (1% [2 of 156]); 6% (9 of 156) of patients had more than one issue. Associations between problematic initial recovery and age, gender, symptoms of anxiety and depression, disease severity, specific exam findings, and insurance were evaluated using t-tests, Mann-Whitney tests, and chi-square tests, with the plan to perform logistic regression if at least two variables had an association with p < 0.10.

Results
The only factor associated with problematic initial recovery was greater symptoms of anxiety (median GAD score 1.5 [interquartile range 0 to 7.8] for problematic initial recovery compared with a median score of 0 [IQR 0 to 2] for nonproblematic recovery; p = 0.04), so we did not perform a logistic regression. Physical examination findings consistent with severe median neuropathy were not associated with problematic initial recovery.

Conclusion
The finding that problematic initial recovery after carpal tunnel release was related to symptoms of anxiety and not to the severity of median neuropathy highlights the need to study the ability of efforts to ameliorate anxiety symptoms before carpal tunnel release as an effective intervention to reduce unplanned visits and additional tests, therapy, and repeat surgery, while improving patient-reported outcomes and experience.

Level of Evidence
Level III, therapeutic study.
Long Term Follow-up of Composite Flaps for Single-stage Reconstruction of Concomitant Tendon and Soft Tissue Defects
Authors: Bekeny Jenna C. , Zolper Elizabeth G. , Dekker Paige K. , Kim Kevin G. , Wang Jessica , Nigam Manas , Attinger Christopher E. , Fan Kenneth L. , Evans Karen K. 
Published: January 2022

Background:
Tendon rupture in the setting of significant soft tissue loss poses a challenging reconstructive situation, which requires (1) recreating a stable gait cycle, (2) reducing shear forces and re-rupture risk, and (3) providing adequate soft tissue coverage. In this study, we outline our experience with composite flaps in single-step reconstruction of various lower extremity tendinous injuries with soft tissue loss.

Methods:
A retrospective review of all patients requiring free tissue transfer at our tertiary wound care center between 2011 and 2020 was performed. Patients undergoing single-stage free tissue transfer for both soft tissue coverage and tendon reconstruction were selected. Variables of interest included demographics, comorbid conditions, baseline functionality, reconstructive details, and wound characteristics. Outcomes of interest were flap success, return to ambulation, time to ambulation, and postoperative complications.

Results:
Nineteen patients were included in this study. Patients were on average 48.0 years old (SD 16.5), with a median Charlson Comorbidity Index of 1.00 (IQR: 0.0–2.5). Defects were most often on the ankle (n = 1 3, 68.4%), with extension to the foot or leg in six of these cases. Median wound size was 68.0 cm2 (IQR: 48.0–120.0). The most common tendon requiring reconstruction was the Achilles (n = 13, 68.4%). An anterolateral thigh flap with attached fascia lata extension rolled into a neotendon was used in all 19 cases. At baseline, all patients were ambulatory. Only one patient (5.3%) required return to the operating room for suspected vascular compromise. At a median of 14.4 months (IQR: 8.5–40.5), all 19 patients were ambulatory.

Conclusions:
Simultaneous reconstruction of tendinous injuries and soft tissue defects can be readily achieved via composite free flaps. Although other methods of reconstruction can be considered for smaller soft tissue and tendon loss, this approach has significant utility for patients with large defects and yields robust return to preinjury functionality.
Characteristics associated with neuropsychiatric symptoms in persons living with dementia and caregiver distress and diminished well-being
Authors: Kovaleva Mariya A. , Higgins Melinda , Dietrich Mary S. , Jennings Bonnie Mowinski , Song Mi-Kyung , Clevenger Carolyn K. , Griffiths Patricia C. , Hepburn Kenneth 
Published: January 2022

ABSTRACT

Background:
The population of persons living with dementia (PLWDs) is increasing, although mainstream dementia care quality is suboptimal.

Purpose:
To identify characteristics associated with: (1) PLWDs' neuropsychiatric symptoms and quality of life; and (2) distress from neuropsychiatric symptoms and well-being among their family caregivers (N = 49).

Methodology:
Cross-sectional single-group examination of PLWD and caregivers when they enrolled into a nurse-led dementia-centered primary care clinic. Pearson correlations were run between characteristics of PLWD and caregiver and variables representing PLWD's neuropsychiatric symptoms and quality of life and their caregivers' well-being. Statistically significant correlations were reported via Cohen d statistics.

Results:
Caregivers' characteristics associated with higher distress from neuropsychiatric symptoms and diminished well-being included Black race, female gender, younger age, caring for a parent with dementia, and being employed. Characteristics of PLWD associated with caregivers' higher distress and diminished well-being included longer time since dementia onset, higher Charlson Comorbidity Index, and non-Alzheimer dementia. Caregivers' characteristics associated with higher neuropsychiatric symptom burden included Black race, female gender, younger age, caring for parent PLWD, and being employed. Characteristics of PLWDs associated with higher neuropsychiatric symptom burden included non-Alzheimer dementia, longer time since dementia onset, more comorbidities, and higher Charlson Comorbidity Index. Finally, a longer time since dementia onset was associated with PLWDs' lower quality of life.

Conclusions:
Black race, caring for caring for a parent with dementia, younger age, and being employed were characteristics linked to PLWDs' higher neuropsychiatric symptom burden and caregivers' diminished well-being.

Implications:
Clinicians must assess and intervene with unpaid caregivers who may not appear obviously distressed.
“Struggling to Accept the New Breast as Part of My Body” – The Challenge of Immediate Breast Reconstruction in Women With Breast Cancer
Authors: Han Jeehee , Jin Juhye , Kim Sanghee , Lee Dong Won , Kim Sue 
Published: January 2022

Background
Women have difficulties in making decisions and experiences an unexpectedly long recovery period after immediate breast reconstruction. There are still many limitations in understanding the overall changes in life experienced by women with breast cancer before and after immediate breast reconstruction.

Objective
This study aimed to explore the challenges experienced by women with breast cancer undergoing immediate breast reconstruction and how they coped with these problems.

Methods
Strauss and Corbin’s grounded theory design was used. In-depth face-to-face individual interviews were undertaken with 11 women with breast cancer from 4 hospitals in Korea. Data collection and analysis were conducted simultaneously, using the constant comparative method.

Results
The core category was “struggling to accept the new breast as part of my body.” “Decision making regarding immediate breast reconstruction” was the initial step, followed by “facing the changed breasts after surgery.” The challenge of “living with the new breasts” slowly merged into “making up my mind to accept my breasts with gratitude.”

Conclusion
Women with breast cancer undergoing immediate breast reconstruction struggled to accept the new breast as part of their body, and some reached a measure of closure and acceptance with gratitude.

Implication for Practice
Nurses can apply findings by recognizing the experiences, perceptions, and needs of women; providing patients with sufficient information on the whole process; and offering continuous supportive care to facilitate the coping process. In addition, nurses should consider a variety of approaches that help women to integrate their changed bodies into their self-concept and self-image and restore a sense of normality.
Lifestyle Behaviors and Parents’ Mental Well-Being Among Low-Income Families During COVID-19 Pandemic
Authors: Robbins Lorraine B. , Ling Jiying 
Published: January 2022

Background
The coronavirus 2019 (COVID-19) pandemic has negatively altered many families’ lifestyles and the mental well-being of parents, especially those who have a low income and young children. To improve low-income parents’ mental well-being, especially during a pandemic, understanding parents’ and children’s lifestyle behaviors and the relationship between their lifestyle behaviors and parents’ mental well-being is essential.

Objective
This cross-sectional study examined relationships between lifestyle behaviors (sleep, physical activity, screen time, and eating behavior of parents and children) and low-income parents’ well-being (stress, anxiety, and depression) during COVID-19.

Methods
Parents were recruited from two Michigan Head Start organizations as well as across the United States; 408 parents completed an online survey. Demographic characteristics were assessed, along with parents’ sleep, physical activity, screen time, and dietary intake; stress, anxiety, and depression were also examined. Children’s sleep time, physical activity, screen time, and fruit/vegetable intake were assessed. Descriptive statistics, correlations, and the multivariate general linear model procedure were used.

Results
Approximately 69.4% of parents reported moderate stress levels, and 17.2% reported high levels. Most parents had sleep disturbances, attained minimal physical activity, and consumed <5 fruits/vegetables per day; average screen time was >2 hours per day. Only 41% of preschoolers were active 7 days a week and slept ≥10 hours per day. Two thirds had >2 hours per day of screen time, and less than one fifth consumed ≥5 fruits/vegetables per day. After adjusting for parents’ demographics and children’s lifestyle behaviors, parents’ sleep disturbance was positively correlated with their levels of stress, anxiety, and depression. After controlling for parents’ demographics and lifestyle behaviors, child sleep time was negatively associated with parents’ stress levels. Family demographics and parents’ and children’s lifestyle behaviors explained 33.4%, 29.8%, and 28.1% of the variances in parents’ stress, anxiety, and depression, respectively.

Discussion
Most parents and preschoolers were not meeting many lifestyle behavior recommendations, indicating a need for interventions. Improving parents’ sleep quality and reducing bedtime challenges involving their preschoolers may be necessary for enhancing parental mental well-being.