bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antenatal and delivery practices and neonatal mortality amongst women with institutional and non-institutional deliveries in rural Zimbabwe: observational data from a cluster randomized trial
Authors: Mooney Ciaran , Noble Christie , Makasi Rachel , Ntozini Robert , Majo Florence , Church James , Tavengwa Naume , Prendergast Andrew , Humphrey Jean 
Published: March 2022

Abstract
Background Despite significant investment and global improvements in neonatal mortality rates over the past three decades, neonatal mortality in Zimbabwe, like in other sub-Saharan countries, has continued to rise. Ensuring access to institutional delivery is an important factor in improving both neonatal and maternal outcomes. MethodsThis study uses data from the Sanitation Hygiene Infant Nutrition Efficacy (SHINE) trial, a cluster-randomized community-based trial, to examine the risk factors, birth practices and infant outcomes among women having institutional or non-institutional deliveries in rural Zimbabwe. Results 11.9% (n=536) fetuses in the study were delivered by 529 mothers outside a health institution. These women were more likely to have a lower socioeconomic status than those who delivered in a health institution. Additionally, hygiene practices were poorer and interventions to minimise neonatal hypothermia less commonly utilised outside of health institutions. Infants with non-institutional deliveries were born on average 1.15 weeks (95%CI 0.84-1.46) earlier than those who were delivered in a health institution; their risk of being born preterm was more than twice as high (RR 2.20, 95%CI 1.92-2.53). The risk of stillbirth and neonatal death among infants born outside a health institution was more than two-fold higher (RR 2.38, 95%CI 1.36-4.15; and 2.01, 95%CI 1.31-3.10, respectively) that among infants born in a health institution. ConclusionsOur findings indicate that premature onset of labor, rather than maternal choice, may be the reason for many non-institutional deliveries in low-resource settings. This most often occurs amongst women from lower socioeconomic backgrounds who cannot reach a health institution quickly, and so are attended by untrained caregivers in less hygienic conditions. This cascade of events results in a two-fold higher risk of stillbirth and neonatal mortality amongst children born outside of a health institution. Interventions for primary prevention of preterm delivery will be crucial in reducing neonatal mortality in Zimbabwe. Trial registration The trial is registered with ClinicalTrials.gov, number NCT01824940.
Retinal Layer Segmentation In A Cohort Of Healthy Children Via Optical Coherence Tomography
Authors: Runge Anna-Katharina , Remlinger Jana , Abegg Mathias , Ferrazzini Thomas , Brügger Dominik , Weigt-Usinger Katharina , Lücke Thomas , Gold Ralf , Salmen Anke 
Published: March 2022

Abstract
Background: High-resolution optical coherence tomography (OCT) allows the detection of macular pathology and involvement of the optic nerve in a wide spectrum of diseases. For the differentiation of diseased and healthy status, normal values of retinal layer segmentation are critical. Yet, normative values mostly cover adult populations with only sparse data for paediatric cohorts. We present data of retinal layer characteristics via OCT in a healthy paediatric cohort.Methods: This prospective cross-sectional study screened 75 healthy children (male=42, female=33, range 4-17 years) without visual problems. OCT was performed with a peripapillary ring and macula scan protocol to determine paediatric normative values for routine parameters (peripapillary retinal nerve fibre layer thickness (pRNFL), total macular volume (TMV), macular retinal thickness (RT)). The macula scan (6mm grid) was segmented using the device-inherent automated segmentation software (Heidelberg Eye Explorer) for retinal layers: RNFL, ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL) in 9 segments each and mean of the 9 segments.Results: We obtained OCT data of 72 children with mean age 12.49 years (standard deviation, SD, 2.18; minimum 3.93). Mean global pRNFL was 102.20 μm (SD 8.24), mean TMV 8.81 mm3 (0.30) and mean RT (all segments) 318.22 μm (10.19). Segmented macular retinal layer thicknesses (mean of all segments) were: RNFL 27.67 μm (2.14), GCL 41.94 μm (2.50), IPL 34.97 μm (2.10), INL 35.18 μm (2.15), OPL 29.06 μm (2.24), ONL 68.35 μm (6.20).Conclusion: The OCT is a useful non-invasive imaging technique for the examination of the retina in children with short duration, high imaging resolution and no known adverse effects. Normative values may serve as a comparator for different neuropaediatric disorders and are first presented with this study using an up-to-date and standardized OCT imaging technique.
The Full-length Genome Sequence of a Novel Amalgavirus in Lillium spp. in China
Authors: Huo Yanyan , Ali Mubassir , Li Xiaoting , Zhou Jiale , Wu Juan , Lin Shengjie , Ding Jiahao , Liu Huan , Yu Jianglian , Hong Ni , Wang Guoping , Ding Fang 
Published: March 2022

Abstract
We report firstly the complete genome sequence of a novel amalgavirus, tentatively designated as ‘Lily amalgavirus 1’(LAV-1), isolated from Lilium spp.in China. LAV-1 is a 3448-nt double-stranded RNA virus. It codes two putative proteins, open reading frame 1(ORF1) is 394 aa in length with unknown function. ORF2 encodes a putative RNA-dependent RNA polymerase (RdRp) of 895 aa. Two ORFs form a ‘1 + 2’ fusion protein translated in ‘+1’ programmed ribosomal frame shift (PRF). BLASTp analysis revealed that the complete genome sequence of LAV-1 shares 48.23%–59.80% sequence identity (query sequence coverage more than 77%) with those of members of the genus Amalgavirus, with the highest nucleotide sequence identity of 59.80% with that of Allium cepa amalgavirus 1 (query sequence coverage 87%). The genome structure, phylogenetic relationships, and sequence similarities to other plant amalgaviruses suggest that LAV-1 is a new member of the genus Amalgavirus.
Effects of Community-Based Family Planning Education Program on Knowledge of Family Planning Methods Among Married Women in Khartoum State, Sudan
Authors: Sagiron Elwaleed , Jarelnape Ahmed 
Published: March 2022

Abstract
Background
Family planning (FP) is an important strategy in promoting maternal and child health. Although family planning was initiated in Sudan in 1965 and then incorporated into the Primary Health Care (PHC) in 1985, the utilization rate remains low, which is among the lowest countries in the region. this study aimed to evaluate the effects of a community-based family planning education program on knowledge of family planning methods for married women in Khartoum State, Sudan.
Methods
Quasi-community interventional study design. It was conducted in Khartoum State, Sudan in December, 2020. A Family planning program was invented as an intervention program. The sample size consists of 456 mothers, the participants in the research are between the ages of (15 and 49). The data were collected by a questionnaire designed to measure mothers’ knowledge. The questionnaire consisted of 30 questions covering the different aspects of family planning. The data were collected by the researcher. A pre-evaluation was done on the knowledge by using a structured questionnaire. After that, the health education program for the experimental group was done within 20 educational hours, pre and post-test is done to the evaluated effectiveness of the educational program.
Results
The study results showed most of the intervention and control groups were illiterate 40%, they have little information about family planning methods, the mothers’ knowledge has increased after family planning program intervention from 40–84.7%.
Conclusion
The study concluded that a family planning educational program has significantly increased a mother’s knowledge regarding family planning.
Genotype and Phenotype of Adenosine Deaminase 2 Deficiency: The Experience From Saudi Arabia
Authors: Alabbas Fahad , Alanzi Talal , Alrasheed Abdulrahman , Essa Mohammed , Elyamany Ghaleb , Asiri Abdulrahman , Almutairi Sajdi , Al-Mayouf Sulaiman , Alenazi Abdullatif , Alsafadi Danyah , Ballourah Walid , Albalawi Naif , Hanafy Ehab , Al-Hebshi Abdulqader , Alrashidi Seham , Albatniji Fatma , Alfaraidi Huda , Ali Tahani Bin , Qwaiee Mansour Al , AlHilali Maryam , Aldeeb Hayam , Alhaidey Ali , Aljasem Hassan , Althubaiti Sami , Alsultan Abdulrahman 
Published: March 2022

Abstract
PurposeDADA2 is a rare and potentially fatal systemic autoinflammatory disease characterized by reduced or absence of ADA2 enzyme activity resulting from mutations in the ADA2 gene. Symptoms of DADA2 are variable and include vasculitis, immune dysregulation, and cytopenia. Scattered Saudi cases of DADA2 have been reported. We describe clinical, molecular, and outcome characterization of Saudi cases focused on phenotypic variability of DADA2.Methods To summarize the clinical features of Saudi patients with DADA2, we conducted a retrospective observational study in the seven major tertiary medical centers. Analysis of clinical characteristics, diagnostic findings, and treatment outcomes was obtained.ResultsWe identified 21 individuals with DADA2 from 14 families. At the time of the study, the patient's age was between 4 and 26 years (median age of 11.3 years). 20 patients presented during childhood. Homozygous c1447-1451del of ADA2 was the most frequently observed mutation in 38%, followed by c882-2A: G in 29%. Absent ADA2 activity was identified in all tested patients with homozygous mutations. Phenotypic manifestations included vasculopathy (stroke or cutaneous lesion) in 48%, cytopenia in 95%, autoinflammatory features such as intermittent fever, transaminases, and hepatosplenomegaly, and or immunodeficiency in 71% of the patients. 4 patients presented with Hodgkin lymphoma and one patient presented with clinical features mimic transverse myelitis, adding to the clinical spectrum of DADA2. Anti-tumor necrosis factor (anti-TNF) is the main treatment. However, regular blood transfusion, splenectomy, cyclosporine, and hematopoietic stem cell transplant (HSCT) were initiated in other patients because of anti-TNF failure. Morality was reported in 3 patients due to fulminant hepatitis and septic multi-organ failure.ConclusionOur cohort expands the variability of molecular and clinical characteristics of DADA2. Hematological phenotype predominates in the patients. Consensus diagnostic criteria are required to facilitate early diagnosis and initiation of prompt therapy. Evaluation of the rare disease complications such as cancer needs large prospective studies or disease registries
Discovery of Novel Inhibitors Targeting HDM2: Virtual Screening, Molecular Dynamics Simulation, Binding affinity predication and Cell Assay
Authors: Cao Feng , Chen Liang , Xiong Feng , Li Hongmei , Liu Xingyong , Zhang Li 
Published: March 2022

Abstract
HDM2(human double-minute 2 protein) is an important drug target for tumor treatment. The main physiological function of HDM2 is to negatively regulate the tumor suppressor protein p53 and inhibit the normal biological function of p53, which leads to the occurrence and development of tumors. Therefore, the discovery of small molecules that can inhibit the activity of HDM2 is a promising cancer therapy. In this study, we reported a virtual screening as an effective strategy to discover potential HDM2 inhibitors from the Specs database. Then 100 ns all-atom molecular dynamics (MD) simulation and binding free energies calculated were carried out on the selected compounds, and cell assay showed that Hit 3 was the most active against SMMC-7721 cell line with an IC50 of 14.14μM. In conclusion, we reported seven prospective candidates with novel skeletons among them Hit 3 is a potential HDM2 inhibitor and provided an insight into the mechanism of interaction of the compounds to HDM2 target.
Duck Circovirus in Northern Vietnam: Genetic Characterization of the Complete Genome and Epidemiological Analysis of the Causative Agent
Authors: Tran Giang Thi Huong , Mai Ngan Thi , Bui Vuong Nghia , Dao Tung Duy , Trinh Dai Quang , Vu Tra Thi Thu , Le Van Phan , Dong Hieu Van 
Published: March 2022

Abstract
In the present study, tissue samples collected from a total of 130 commercial ducks collected from clinically suspected flocks and diseased birds in six provinces of northern Vietnam were tested for DuCV infection. The DuCV genome was detected in 56 out of 130 (43.08%) duck samples by PCR. Of 38 tested farms, 26 (68.42%) were positive for the DuCV genome. The rate of the DuCV genome detection in ducks at 3–4 (54.17%) is significantly higher (p<0.05) than that of <3 (32.43%) and >7 (33.33%), insignificantly higher than that of 5–7 (43.33%) (p=0.11) weeks of age. Length of the six Vietnamese DuCV genomes obtained ranged from 1,988 to 1,995 nucleotides. Among the six Vietnamese DuCV genomes, nucleotide identities were from 83.24% to 99.69%. Phylogenetic analysis of the complete genomes indicated that the DuCVs circulating in northern Vietnam were divided into two main genotypes I and II, and several subgenotypes. The Vietnamese DuCVs were closely related to the Chinese, Taiwanese, and Korean strains. One positive selection site of capsid protein was detected.
Knockdown long non-coding RNA PCAT7 inhibits proliferation, migration and invasion of luminal B breast cancer cells via cell cycle arrest
Authors: Zhu Fangyuan , Shao Yingbo , Chen Qi , He Yaning , Liu Chaojun , Nie Bing , Liu Hui 
Published: March 2022

Abstract
Breast cancer is the most commonly diagnosed cancer and one of the leading causes of cancer death. In recent years, an increasing number of studies have shown that the dysregulation of long non-coding RNAs has vital effects on the progression of breast cancer. But the function of PCAT7 in luminal B breast cancer has not been explored. This study aims to investigate the expression of PCAT7 in luminal B breast cancer tissues and cell lines and the effects of PCAT7 knockdown in luminal B breast cancer cells. We found that PCAT7 was dramatically up-regulated in luminal B breast cancer tissues and related cell lines. Knockdown of PCAT7 significantly inhibited cell proliferation, migration and invasion of luminal B breast cancer cells. Cell cycle assay showed that PCAT7 knockdown induced G0/G1 phase arrest of the luminal B breast cancer cells. Moreover, the relative expression of p27 protein significantly increased after PCAT7 knockdown. In conclusion, knockdown of PCAT7 inhibits the proliferation, migration, and invasion of luminal B breast cancer cells via cell cycle arrest.
Computational analysis of potential candidate genes involved in the cold stress response of ten Rosaceae members
Authors: Shafi K. Mohamed , Sowdhamini Ramanathan 
Published: March 2022

Abstract
Background: Plant species from Rosaceae family are economically important. One of the major environmental factors impacting those species is cold stress. Although several Rosaceae plant genomes have recently been sequenced, there have been very few research conducted on cold upregulated genes and their promoter binding sites. In this study, we used computational approaches to identify and analyse potential cold stress response genes across ten Rosaceae family members. Results: Cold stress upregulated gene data from apple and strawberry were used to identify syntelogs in other Rosaceae species. Gene duplication analysis was carried out to better understand the distribution of these syntelog genes in different Rosaceae members. A total of 11145 popular abiotic stress transcription factor-binding sites were identified in the upstream region of these potential cold-responsive genes, which were subsequently categorised into distinct transcription factor (TF) classes. MYB classes of transcription factor binding site (TFBS) were abundant, followed by bHLH, WRKY, and AP2/ERF. TFBS patterns in the promoter regions were compared among these species and gene families, found to be quite different even amongst functionally related syntelogs. A case study on important cold stress responsive transcription factor family, AP2/ERF showed less conservation in TFBS patterns in the promoter regions. This indicates that syntelogs from the same group may be comparable at the gene level but not at the level of cis-regulatory elements. Therefore, for such genes from the same family, different repertoire of TFs could be recruited for regulation and expression. Duplication events must have played a significant role in the similarity of TFBS patterns amongst few syntelogs of closely related species.Conclusions: Our study over all suggests that, despite being from the same gene family, different combinations of TFs may play a role in their regulation and expression. The findings of this study will provide information about potential genes involved in the cold stress response, which will aid future functional research of these gene families involved in many important biological processes.
Progressive crushing 40Ar/39Ar dating of gold-bearing quartz vein from the Liaotun Carlin-type gold deposit at the northwest of Guangxi
Authors: Hu Rongguo , Pang Baocheng , Bai Xiujuan , Bai Lingan , Liu Xijun , Li Yuanqiang , Xu Jianqi , Qiu Huaning 
Published: March 2022

Abstract
Progressive crushing 40Ar/39Ar dating has been performed on a gold-bearing quartz vein from the Liaotun Carlin-type gold deposit at the northwest of Guangxi for the first time. Gases liberated from the secondary and primary fluid inclusions yielded two age plateau with ages of 168.4 ± 1.9 Ma and 200.5 ± 1.9 Ma, respectively. The data points constituting the age plateau yielded excellent inverse isochrons with isochron ages of 167.0 ± 1.9 Ma and 200.7 ± 2.1 Ma. The initial 40Ar/36Ar ratios corresponding to the two isochrons are 308.9 ± 6.8 and 298.0 ± 4.3, which are statistically indistinguishable from the air, indicating that the ore-forming fluids probably mainly derived from the basin pressure flow dominated by atmospheric precipitation. The age from the PFIs of 200.5 ± 1.9 Ma can be taken as the timing of Au mineralization. In contrast, the age of 167.0 ± 1.9 Ma recorded by SFIs represents the timing of late hydrothermal fluid superposition and transformation after the formation of gold deposit. Our preliminary study shows the feasibility and great potential of 40Ar/39Ar dating by progressive crushing of fluid inclusions from quartz vein to date the mineralization age and decipher the fluid origins of Carlin-type gold deposits.
Assessing Fine Motor and Sensory Functions In Patients With Breast Cancer At Risk for Chemotherapy Induced Peripheral Neuropathy (CIPN)
Authors: Tuğral Alper , Arıbaş Zeynep , Akyol Murat , Bakar Yeşim 
Published: March 2022

Abstract
Purpose: This study was aimed to assess potential Chemotherapy-Induced Peripheral Neuropathy (CIPN) related motor and sensorial deficits in patients with breast cancer undergoing chemotherapy. This study was registered to Clinical Trials with the following unique number: NCT04799080.Methods: 27 female patients (Mean age: 51.59±10.55 year) were assessed in three different time points as follows: T1 (before chemotherapy), T2 (interim analysis), and T3 (completion of chemotherapy). Motor function was assessed with handgrip strength (HGS) and peripheral muscle strength (PMS). Semmens Weinstein Monofilament Test was used to assess the potential sensory loss. Minnesota Manual Dexterity Test (MMDT) was used to assess fine motor function. Fatigue was assessed with the European Organization for Research and Treatment of Cancer Quality of Life Module Cancer Related Fatigue (EORTC-QLQ-FA12)Results: HGS was not found significant among all three assessment time points (F (2,52) =0.590, p=0.558). PMS was found significant (F (2,52) =4.058, p=0.023). Bonferroni post-hoc test revealed that PMS was significantly decreased between T1 and T2 (170.38±30.79 N vs 154.41±23.79 N, mean difference=15.972, p=0.046, respectively). Cutaneous sensory tactile function (p>0.05) and MMDT (F (2,52) =0.789, p=0.460) did not change significantly from T1 through T3. In T3, fatigue and PMS was found significantly correlated (r=-0.636, p=0.002).Conclusions: CIPN related problems affect a great number of cancer survivors. Establishing knowledge and awareness associated with CIPN as well as its early recognition and detection might improve patient-centered clinical outcomes in oncology setting.
The NEOM Brine Pools – The First Discovery in the Gulf of Aqaba
Authors: Purkis Sam , Shernisky Hannah , Swart Peter , Sharifi Arash , Oehlert Amanda , Marchese Fabio , Benzoni Francesca , Chimienti Giovanni , Duchâtellier Gaëlle , Klaus James , Eberli Gregor , Peterson Larry , Craig Andrew , Rodrigue Mattie , Titschack Jürgen , Kolodziej Graham , Abdulla Ameer 
Published: March 2022

Abstract
We present the NEOM Brine Pools, the first complex of brine pools discovered in the Gulf of Aqaba. The discovery was made at 1,770 m water depth and consists of one large pool (10,000 m2) flanked by three minor ones (<10 m2). Situated immediately at the toe-of-slope, the largest of the NEOM brine pools episodically receives terrestrial outwash from the Saudi coastal plain. A transect of cores through this pool’s bed reveals a stratigraphy spanning the last 1,200 yrs. Major terrestrial inputs to the basin are recorded once per century, which we attribute to tsunami. Turbidite beds, meanwhile, deposit every 25 yrs. and likely record both flashfloods and the pervasive seismicity of the Aragonese Deep, the pull-apart basin in which the pools situate. Such signals are exquisitely preserved beneath the pools as bioturbating organisms cannot occupy the harsh hypersaline, anoxic brine. These observations extend the known geographical range of Red Sea brine pools, introduce a new sediment archive of event horizons, and document a new bathyal ecosystem in the Gulf of Aqaba; ultimately providing a range of significant data that will contribute to the reconstruction of more than one millennium of preserved turbidites, flashfloods, and tsunami sedimentary deposits.
Genome-wide association mapping of agronomic and quality traits in foxtail millet
Authors: Lou Yanhong , Chen Yun , Chao Ying , Han Fei , Sun Mingjie , Pan Hong , Wang Hui , Liu Zhihao , Zhuge Yuping 
Published: March 2022

Abstract
Foxtail millet [Setaria italica L.] is a particularly momentous cereal and forage crop in arid and semi-arid regions, and was recognised worldwide for its potential as a health-promoting functional food. An understanding of the genomic variation and alleles underpinning its agronomic and quality traits is important for improving foxtail millet breeding. Therefore, high-quality single nucleotide polymorphisms (SNPs) to perform a genome-wide association study (GWAS) was employed to better understand the diversity of foxtail millet and facilitate the genetic dissection of its key traits. Using genotyping-by-sequencing, 107 foxtail millet accessions were sequenced; further analysis revealed 72,181 high-quality SNPs, 46 of which were significantly associated with 13 agronomic and quality traits. These SNPs were distributed across the 8 chromosomes; 36 were located in intergenic regions, whereas 1 and 5 SNPs were located in exon and intron regions, respectively. The GWAS revealed that 28 SNPs were associated with a single trait (Peak time). For some of the significant SNPs, favourable genotypes showed pyramiding effects for several traits. The 46 loci identified in present study will therefore be helpful for breeding plans aimed at foxtail millet improvement. Overall, the results provide sufficient fundamental for excellent cultivar breeding in the sustainable development of foxtail millet.