bluuebunny/crossref_metadata_2025_split huggingface dataset:
Illumina MiSeq sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests
Authors: TCHIECHOUA Yves Hermandez , Odee David W. , Ngonkeu Eddy L. M. , Kinyua Johnson , Ngumi Victoria W. , Machuka Eunice M. , Waweru Bernice , Roux Marcellous Le , Pelle Roger 
Published: December 2022

Abstract
Purpose
Arbuscular mycorrhizal fungi (AMF) play a key role in the phytochemical modulation of medicinal plant species, besides their ecological role in shaping plant communities. Knowledge about the diversity and structure of AMF species associated with the endangered Prunus africana is valuable in the conservation and domestication of the species for its medicinal products.
Methods
Thus, we investigated the diversity and structure of AMF species communities in the rhizosphere soils of P. africana trees occurring in four fragmented Afromontane forests found in Cameroon; (Mount Cameroon and Mount Manengouba) and Kenya (Chuka and Malava) using Illumina Miseq of 18S rRNA gene amplicons.
Results
The forward and the reverse reads were A total of 64 virtual taxa (VT) belonging to eight genera were detected, namely Glomus (43 VT), Claroideoglomus (6 VT), Paraglomus (5 VT), Acaulospora (4 VT), Diversispora (3 VT), and Archaeospora, Pacispora, and Scutellospora with 1 VT each. Scutellospora heterogama VTX00286 was the most abundant and common species in all four sites (49.62%). Glomeraceae and Gigasporaceae were the most abundant families found across the sites, while Acaulosporaceae, Pacisporaceae, and Archaeosporaceae were rare, represented by <1% of all the detected taxa.
Conclusion
Our data show a high diversity of AMF species associated with P. africana, and variable community structure partially shaped by local edaphic factors. This study provides preliminary information on the possibility of using indigenous AMF inoculum, for the cultivation and domestication of P. africana for the sustainable management of this important medicinal plant.
Morphological variation of Rumex longifolius DC. in the Alps and the mountains bordering Bohemia
Authors: BerndSonnberger  
Published: December 2022

Abstract
Neophytic populations of Rumex longifolius (Northern Dock) in the Alps and the mountain ranges bordering Bohemia differ in the length-width ratio of the basal leave blades, the shape and size of the valves and the tendency to develop tubercles of the valves. Five discrete populations from the Czech Republic (Krkonoše moutains and Ještěd mountain), Germany (Bavarian Forest), Austria (Arlberg mountain) and Switzerland (Engadin) are described by quantitative evaluation of specimens collected in the years 2014 – 2018 and their offspring cultivated in the Botanical Garden of Frankfurt a. M. for two vegetation periods. They have probably originated by independent introduction events. The Engadin population shows features of an introgression by Rumex crispus. It is possibly native there, which, however, can be concluded by indirect evidence only. The morphology of specimens from the upper Tyrolian Inn valley in the herbarium of the Natural History Museum Vienna suggests their origin from the Engadin, which should be confirmed statistically by additional collections. The taxonomical significance of subsp. sourekii Kubát which has been described based on a single character (leaf shape) is questioned considering the polymorphism of the species, and the seasonal dependence of the leaf width observed in the cultivation experiments. The observed morphological variability and ecological preferences of R. longifolius could be an indication of its experimentally yet not proved hybrid origin from R. aquaticus and R. crispus.
3-Hydroxy-3-methylglutaryl (HMG)-coenzyme A (CoA) reductase inhibitor modulates biomarkers related to Alzheimer's disease pathology in a sepsis-surviving rat model
Authors: Catalão Carlos Henrique Rocha , Costa Luis Henrique Angenendt da , Santos Jonathas Rodrigo dos , Alberici Luciane Carla , Falconi Luiz Luciano Sobrinho , Coimbra Norberto Cysne , Barichello Tatiana , Rocha Maria José Alves 
Published: December 2022

Abstract

Sepsis survivors have persistent neurological changes, including cognitive and behavioral dysfunction, which are associated with increased production of neurodegenerative biomarkers and morphological changes in areas with mnemonic functions. 3-hydroxy-3-methylglutaryl (HMG)-coenzyme A (CoA) reductase inhibitors have been proposed as a potential therapeutic approach in sepsis, given their anti-inflammatory and antioxidant properties. Here we investigated the possible neuroprotective effect of an HMG-CoA reductase inhibitor (simvastatin) by analyzing neurodegenerative markers, mitochondrial respiration, and neuronal tracing in the hippocampus, prefrontal cortex, and thalamic nucleus reuniens (RE) of sepsis survivor animals. Adult Wistar rats (280 ± 30 g) were submitted to sepsis by cecal ligation and puncture (CLP, n = 28) or left as non-manipulated (control, n = 24). The animals were treated with simvastatin (20 mg/kg) or vehicle four days before and ten days after surgery. The treatment recovered expression of Smad-3 in hippocampus (F(3, 28) = 6.22; P < 0.05), and prevented increased expression of calpain-1 (hippocampus (F(3, 28) = 16.06; P < 0.0001; prefrontal cortex: F(3, 28) = 10.54; P < 0.05) and GSKβ (hippocampus: F(3, 28) = 62.79; P < 0.0001; prefrontal cortex: F(3, 28) = 15.35; P < 0.0001) in the brain structures of the sepsis survivor animals. Septic animals showed mitochondrial dysfunction and a decrease in axon terminals in the RE. Simvastatin seems to restore energy metabolism by improve of the ETS values in the hippocampus (F(3, 12) = 7.533; P < 0.01) and the P/E ratio in the prefrontal cortex (F(3, 12) = 5.818; P < 0.05), in addition to preventing the reduction of axon terminals in survivor animals. These results together suggest a potential neuroprotective effect of simvastatin and raise the importance of considering HMG-CoA reductase inhibitors as a possible adjuvant therapy in sepsis.
RILS-ROLS: Robust Symbolic Regression via Iterated Local Search and Ordinary Least Squares
Authors: Kartelj Aleksandar , Djukanović Marko 
Published: December 2022

Abstract
In this paper, we solve the well-known symbolic regression problem that has been intensively studied and has a wide range of applications.
To solve it, we propose an efficient meta-heuristic-based approach, called RILS-ROLS. RILS-ROLS is based on the following two elements: (i) iterated local search, which is the method backbone, mainly solving combinatorial and some continuous aspects of the problem; (ii) ordinary least square method, which focuses on the continuous aspect of the search space -- it efficiently determines the best-fitting coefficients of linear combinations within solution equations. In addition, we introduce a novel fitness function that combines important model quality measures: R2 score, RMSE score, size of the model (or model complexity), and carefully designed local search, which allows systematic search in proximity to candidate solution.
Experiments are conducted on the two well-known ground-truth benchmark sets called Feynman and Strogatz. RILS-ROLS was compared to 14 other competitors from the literature. Our method outperformed all 14 competitors with respect to the true symbolic model accuracy under varying levels of noise. It also proved to be the most efficient method considering the average running time of reaching the exact model. In addition to evaluation on known ground-truth datasets, we introduce a new randomly generated set of problem instances. The goal of the Random dataset was to test the scalability of our method with respect to incremental equation sizes and number of variables, under different levels of noise.
The statistical analysis of obtained experimental results confirmed that RILS-ROLS can be considered as a new state-of-the-art method for solving the problem of symbolic regression when ground-truth equations are known.
Radioterapia śródoperacyjna z wykorzystaniem elektronów
Authors: Graczyk Kinga , Kruszyna-Mochalska Marta , Kijeska Weronika 
Published: December 2022

Taktyki leczenia, które łączą ze sobą oszczędzającą operację chirurgiczną z radioterapią stały się standardem w leczeniu wczesnych nowotworów. Jednym z przykładów skojarzonego leczenia jest wykonanie radioterapii śródoperacyjnej, którą przeprowadza się w trakcie zabiegu chirurgicznego, gdy pacjent nadal znajduje się pod anestezją w sterylnej sali operacyjnej.
Celem pracy jest omówienie techniki radioterapii śródoperacyjnej wykorzystującej wiązkę elektronów (IOERT) generowaną przez mobilne akceleratory.
Metoda IOERT znajduje swoje zastosowanie w leczeniu nowotworów takich lokalizacji jak: pierś, prostata, trzustka, okrężnica i odbytnica. Na podstawie doniesień literaturowych omówione zostało: przebieg procedury, najczęściej stosowane parametry fizyczne oraz wyzwania związane z kontrolą jakości i ochroną radiologiczną personelu.
Endometriosis Painful Symptoms and its Relationship with Quality of Life
in Women Referring to Educational Centers of Tabriz University of Medical
Sciences
Authors: Alizad Sevda , Mirghafourvand Mojgan , Oskouei Behnaz Sadeghzadeh , Bani Soheila 
Published: December 2022

Background:
Endometriosis is one of the most frequent illnesses and is connected with
substantial problems, such as pain and reduced social function.

Objective:
This study aimed to examine the association between painful endometriosis symptoms and
women's quality of life.

Methods:
This research included 169 endometriosis patients. Data was collected using sociodemographic,
ENDO PAIN- 4D, and EHP-30 questionnaires. To examine the relationships between
pain symptoms and quality of life, the Pearson correlation test was performed. Also, the general linear
model with controlling the probable confounding variables was used for multivariate analysis.

Results:
The mean (SD) of the overall ENDOPAIN 4D and the overall EHP 30 scores were 66.0
(61.0), and 35.9 (22.4), respectively. In terms of quality of life, the emotional domain had the greatest
42.0 (20.3), and the pain domain had the lowest mean score 7.2 (4.5). The total score of EHP 30 and
ENDOPAIN 4D had a substantial positive connection (r = 0.55 and p > 0.001). With an increasing
ENDOPAIN 4D score, the quality-of-life score increased (B = 0.2; CI: 95 %: 0.1 to 0.2). Explaining
that in the EHP-30 scale, the quality of life worsens as the score increases.

Conclusion:
This study found that the more intense the painful symptoms of endometriosis, the poorer
the quality of life. Thus, it is vital to lessen the painful symptoms and practice self-care to enhance
these patients' quality of life.
Nalbuphine Suppresses Leukemia Stem Cells and Acts Synergistically with Chemotherapy
Drugs via Inhibiting Ras/Raf/Mek/Erk Pathway
Authors: Dan Jiapeng , Xiao Jinliang , Wang Weilian 
Published: December 2022

Aims:
Retrospective clinical studies have shown that opioids could potentially affect the risk of cancer recurrence and metastasis. Better understanding of the effects of opioids on cancer will help to select the optimal anesthetic regimens to achieve better outcomes in cancer patients.

Background:
Increasing evidence has shown the direct effects of opioids on bulk cancer cells and cancer stem cells. Opioid such as nalbuphine is approved to control cancer-associated pain but little is known on their possible cancer effects.

Objective:
To assess the biological effects of nalbuphine on acute myeloid leukemia (AML) differentiated and stem/progenitor CD34+ cells.

Method:
AML CD34+ cells were isolated with colony formation, growth and apoptosis assays performed. Biochemical and immunoblotting analyses were conducted in AML cells exposed to nalbuphine.

Result:
Nalbuphine at clinically relevant concentrations was active against a panel of AML cell lines with varying IC50. Importantly, nalbuphine augmented the efficacy of cytarabine and daunorubicin in decreasing AML cell viability/growth. Besides bulk AML cells, we noted that nalbuphine was effective and selective in decreasing viability and colony formation of AML CD34+ cells while sparing normal hematopoietic CD34+ cells. The action of nalbuphine on AML cells is not associated with opioid receptors but via inhibiting Ras/Raf/MEK/ERK signaling pathway. Overexpression of constitutively active Ras partially but significantly reversed the inhibitory effects of nalbuphine on AML cells.

Conclusion:
Our findings reveal the selective anti-AML activity of nalbuphine and its ability in inhibiting Ras signaling. Our work suggests that nalbuphine may be beneficial for leukemia patients.
In vitro and In vivo Investigations on Anti-Hyperuricemic Activity of
Commercial Preparations of Persicaria minor (Biokesum®) and Eurycoma
longifolia (Physta®)
Authors: Chinnappan Sasikala M. , George Annie , Choudhary Yogendra Kumar , Godavarthi Ashok , Teng Chi-Ling , Jin Wong Hoi 
Published: December 2022

Background:
Eurycoma longifolia Jack (Simaroubaceae) root extracts and Persicaria
minor Huds. (Polygonaceae) leaf extracts are known to exhibit antioxidant activity, while their antihyperuricemic
effects remain unclear.

Objective:
The objective of this study is to investigate the anti-hyperuricemic activity of Biokesum®
(extract of P. minor) and Physta® (extract of E. longifolia).

Methods:
In vitro anti-hyperuricemic activity for Biokesum® and Physta® was measured by xanthine
oxidase inhibition based on a biochemical enzymatic assay at concentrations of 1, 10, and 100
μg/mL. In vivo anti-hyperuricemic activity was assessed in potassium oxonate-induced hyperuricemia
Sprague-Dawley rat model. Biokesum® at doses of 100, 300, and 500 mg/kg and Physta® at
doses of 300 and 500 mg/kg were administered for 7 days to the hyperuricemic rats. Serum uric acid
was measured on days 0, 1, 3, and 7, and liver xanthine oxidase activity was measured on day 8.
Allopurinol was used as positive control in both in vitro and in vivo investigations.

Results:
Based on xanthine oxidase biochemical enzymatic assay, IC50 of Biokesum® and Physta®
were 88.6 μg/mL and > 100 μg/mL respectively. In hyperuricemic rats, Biokesum® and Physta®
significantly reduced the serum uric acid by 5.6% and 5.5% respectively at 300 mg/kg (p < 0.05)
and 9.8% and 9.0% respectively at 500mg/kg (p < 0.001) compared to untreated rats. Significant
liver xanthine oxidase inhibition at 37.5% and 34.1% were observed in rats treated with Biokesum®
and Physta® respectively.

Conclusion:
The study has demonstrated hyperuricemic activity of Biokesum® and Physta®, clinical
studies in hyperuricemia-related diseases population are required to further confirm the activity.
Evaluación financiera de la producción in vitro de árboles de olivo con opciones reales
Authors: Perea-Gómez Lorena , García-Salazar José Alberto , López-Reyna María del Carmen , Arellano-Ostoa Gregorio 
Published: December 2022

Los proyectos para establecer empresas biotecnológicas son difíciles de evaluar porque se encuentran en escenarios de riesgo e incertidumbre. El objetivo de este trabajo fue evaluar la viabilidad financiera de un laboratorio comercial de cultivo in vitro para propagar árboles de olivo (Olea europaea L.). El proyecto se evaluó con el método de análisis financiero a través del cálculo de indicadores como el valor actual neto (VAN), la tasa interna de retorno (TIR), la tasa de actualización (TREMA) y la relación beneficio costo (B/C), y con el enfoque de opciones reales utilizando arboles binomiales. Este último método consideró la volatilidad de precio del producto y la probabilidad de éxito de cada una de las cinco etapas (establecimiento, multiplicación, enraizamiento, aclimatación y vivero) del proyecto. Los resultados obtenidos en el análisis financiero indicaron que el proyecto se rechazó pues el VAN fue de MXN -1 498 656, la TIR fue de 13%, la TREMA de 25% y la B/C de 0.83. Los resultados de la evaluación con el método de opciones reales indican que cada una de las cinco etapas del proyecto se podría llevar a cabo en los dos escenarios analizados, ya que el VAN total fue positivo en cada etapa y, además, el valor de la etapa siguiente fue mayor al valor de la anterior. Con este método se pudo determinar el valor real del proyecto y el momento oportuno de abandonarlo; por lo tanto, se recomienda a los inversionistas utilizarlo al momento de tomar decisiones en inversiones de alto riesgo de proyectos biotecnológicos.
Adopting Incremental Innovation Approaches in the Digitalization of Village Government Services
Authors: Ahmad Jamaluddin 
Published: December 2022

Although much research has established the importance of digitalization in public services, in practice, village governments responsible for village community services often obscure public perceptions about the potential impact on digital-based public services. This study aims to provide a more detailed picture of the incremental innovation approach in the implementation of public services, especially the adoption of digital technology in the delivery of village government service. This research is a case study research that uses a qualitative research design. study results underline the importance of the incremental innovation approach. Digitalization impacts public perceptions about transparency, speed, and accountability; willingness to accept changes, especially changes in the use of mobile phones (mobile phones) as needed; and resultant social impact. Thus, study results are an invaluable input into the decision-making process related to public services.
Schools’ Perceived Readiness in Responding to Employment Policy for Person with Disability
Authors: Kustulasari Agustina , Pramahayekti Gupita , Setiawan Thomas Julio Aji 
Published: December 2022

Law Number 8 of 2016 concerning Persons with disability in Indonesia has been ratified since 2016, with one of the objectives is to expand employment opportunities for persons with disabilities. This study aims to identify the schools' perceived readiness understudy in responding to the employment policy (quota of workers with disabilities of 1% for private companies and 2% for government, regional government, BUMN, and BUMD) through the implementation of vocational-based education. This study compares a number of Special Schools (SLB) and Vocational High Schools (SMK) in the Special Region of Yogyakarta to provide an overview of the perceived readiness of 2 types of schools that serve education for children with special needs. In measuring the level of perceived readiness, this research drew seven aspects: curriculum, learning process, teaching staff, management, funding, infrastructure, and environment, with an additional aspect of student input SMKs. This research is a quantitative study with a survey approach. The study found that schools perceived themselves ready, but this may be bound to their common practice and understanding of inclusive education. This study hopes to contribute to the literature and policy discussions on special education and the employment of persons with disabilities.
CAN A PROPER STRATEGIC PLANNING SECURE ITS IMPLEMENTATION? A CASE IN NEW DISTRICT IN DEVELOPING COUNTRY
Authors: Hidayati Firda 
Published: December 2022

Strategic planning is highly recommended as one of the key factors that determine the success of the organization. However, only a few organizations succeed in implemented it. The research studied causes of poor strategy implementation. The study used Data collection techniques included previous research on factors that influence strategy implementation failures, and a survey of . three levels of managers in 38 agencies in the government. Using structural Equation Modelling-Partial Least Squares (SEM-PLS) as an analysis tool, results showed that stakeholder uncertainty, resources, and expertise are the major factors that influence the failure of strategy implementation. The finding provides an important explanation for poor strategy implementation performance in Batu Bara , which is a new district in Indonesia. One of the key policy recommendations is for the government to take measures toward enhancing the capacity of managers to manage stakeholders’ uncertainty.
Sustainable Coral Reef Ecosystem Management in Palopo City Territorial Waters
Authors: Bibin Muhammad 
Published: December 2022

Damage to coral reefs in the waters of Palopo City poses danger to the productivity of the habitat. Therefore, carrying out the adopting the principles of a conservation in the use and management of coral reefs in Palopo City area waters through an integrated system and participation of multi-stakeholders is considered imperative. The study analyzed the level and causes of damage coral reefs, assessed the role of stakeholders in managing coral reef ecosystems, and formulated a strategic model for managing coral reef ecosystems in Palopo City waters. Methods of data analysis included assessment of coral reef condition, stakeholder analysis, and hierarchical analysis. Result showed that fishing activity using environmentally unfriendly gear such as fish bombs and potassium is one of the principal causes of damage to coral reefs in the waters of Palopo City. Most damage occured to coral reefs at a depth of 4 meters at station 1, which registered a the percentage of the mortality index of 82.07%. Meanwhile, the least damage was 69.73%. At the depth of 4 meters station two is Result of stakeholder mapping identified three groups inter alia, contest setters, key players, and subject. The first priority strategy in the management of coral reef ecosystems in the waters of Palopo City should be designate the zoning of coastal areas, coordination among stakeholders that are relevant and important with respect to coral reef ecosystems power relations, drafting local regulations that mandate sustainable use and management of coral reef ecosystems and increasing training programs for fishing communities.
Software Package for Remote Diagnostics of Agricultural Machinery Condition
Authors: Kostomakhin M. N. , Pestryakov E. V. 
Published: December 2022

Artificial intelligence is stated to be more and more widely used in agriculture, as well as for the diagnostics of the agricultural machinery condition. It was noted that in besides software, new computing devices are developed that enable processing and storing large amounts of data. (Research purpose) To create a neural network-based software package for remote diagnostics of the limit state of machinery individual components and assemblies. (Materials and methods) Foreign studies within the problem area were analysed. It was found out that for data collection for artificial intelligence there exist STM32 and Arduino microcontroller-based devices, and the Nvidia CUDA (Compute Unified Device Architecture) hardware and software platform is used. For the software was developed in the C / C ++ programming language, and the MS SQL Server database were used as a repository. The general software is emphasized to be able to run on all major operating systems such as Windows, Mac OS, Linux. The role of neural network is argued to be important since it integrated all program blocks and provides its own analysis. (Results and discussion) The information from the diagnostics devices is accumulated in a database. The neural network created on the basis of this database is constantly learning and simultaneously analyzing incoming data in real time, automatically issuing its recommendations. It was found that the neural network created by the employees of the Federal Scientific Agroengineering Center VIM has more functional options, for example, it is able to work directly with devices and conduct a more detailed technical analysis. (Conclusions) A neural network for equipment condition diagnostics was created, which increases the efficiency of decision-making in case of repair, and improves forecast and predictability. The criteria for equipment operation were proposed.