bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digitalization Of Tax Administration Systems And Tax Complience Costs On Taxpayer Complience Of Individual Taxpayer
Authors: Wulandari Dian Sulistyorini 
Published: May 2022

This study aims to examine the effect of the application of e-filing, e-billing, e-invoicing, and tax compliance costs on taxpayer compliance. The independent variable in this research are e-filing, e-billing, e-invoicing, and tax compliance, while dependent variabe is taxpayer compliance. The population in this research was an individual taxpayer listed in KPP Pratama South Cikarang. The sample in this study is determined by random sampling method with a sample size of 100 respondents. the primary data collectin method used in this study is a survey method with questionnaire media. The method of analysis used is multiple linear regression. The results of this research is shows that the effect of the application of e-filling and e-invoicing has a positif effect on taxpayer compliance. Tax compliance cost have a negative effect on taxpayer compliance. While the effect of the application of the e-billing has no effect on taxpayer compliance.
Rumen microbiota alterations during subclinical ketosis is associated with the development of subclinical mastitis in dairy cows
Authors: Xiang Kaihe , Shen Peng , Mu Ruiying , Li Shuang , Wang Ying , Zhao Caijun , Zhang Naisheng , Hu Xiaoyu , Fu Yunhe 
Published: May 2022

Abstract
Background: Mastitis is the most serious disease endangering animal husbandry, especially dairy farming. Clinical investigations indicated that cows suffering from ketosis have a higher probability of mastitis. The composition of rumen microbiota is closely related to ruminant health. However, it is not clear what role it plays in this process.Results: Blood β-hydroxybutyrate (BHBA), blood non-esterified fatty acids (NEFA), milk somatic cell counts (SCC), milk fat, fat to protein ratio, and milk urea nitrogen (MUN) were significantly elevated in all cows with subclinical ketosis (SCK)，while the milk lactose was significantly decreased. The production and proportion of volatile fatty acids (VFA) in rumen changed during SCK, with significant decreases in acetate, propionate, butyrate, and total VFA (TVFA), and a significant increase in the acetate to propionate ratio. The microbiota in rumen fluid and milk were determined by 16S rRNA gene sequencing. The results showed that the richness of rumen microflora was significantly decreased during SCK. The rumen harbored unique bacterial community in cows suffering SCK, which were significantly correlated with BHBA in plasma, SCC in milk and milk components. LEfSe analysis revealed that it was characterized by the increase of Bacteroidota, Christensenellaceae_R-7_group, Luteimonas and Thermomonas and the decrease of Prevotella. The richness of milk microflora was significantly increased during SCK. LEfSe analysis suggested a significant increase in Staphylococcus and a significant decrease in Coxiella, Acinetobacter, Oscillospiraceae_UCG-005 and Corynebacterium. Conclusions: The SCC of cows with subclinical ketosis was significantly elevated and all individuals suffered from subclinical mastitis. The rumen microbiota during subclinical ketosis changed significantly, characterized by decreased richness, increased acetate and butyrate producing bacteria and decreased propionate producing bacteria, and was closely related to blood BHBA and milk SCC. This provides a new perspective for the study of the relationship between subclinical ketosis and subclinical mastitis as well as the prevention and treatment of the two diseases.
Immediate Effect of Trunk Flexion and Extension Isometric Exercise Using an External Compression Device on Electromyography of the Hip Extensor and Trunk Range of Motion of Healthy Subjects
Authors: Huang Tian-zong , Kim Suhn-yeop 
Published: May 2022

Abstract
Background: Sacroiliac joints (SIJs) transmitted trunk load to lower extremities through the lumbopelvic. External compression devices across the SIJs could provide stability to the SIJs. A previous study established that using a device known as Active Therapeutic Movement version 2 (ATM®2) has been developed to improve pain and joint range of motion (ROM) in patients with LBP. However, no study has examined the physiological change in the muscle through ATM®2-based exercise thus far. This study aimed to determine the immediate effects of ATM®2 exercise on the contraction timing, back extension endurance, muscle fatigue, and trunk ROM of lumbar and lower limb muscles in healthy subjects.Methods: Thirty-six healthy subjects (mean age=23.16±2.3) volunteered to participate in this study. Subjects were instructed to perform ROM test using sit and reach test, back extensor endurance test using Biering-Sorensen test, erector spinae (ES), lumbar multifidus (LM) fatigue and onset time of Gluteus maximus (GM) in prone hip extension using electromyography before and after trunk flexion and extension isometric exercises.Results: The ROM in trunk flexion showed a significant increase of 7.9% after exercise compared to that before exercise (p<0.05). Relative GM contraction onset timing significantly decreased after exercise (p<0.05). The result of the Sorensen test after exercise showed a trend of increase in duration time. Muscle fatigue in the LM, however, showed a significant increase (p<0.05), whereas muscle fatigue in the ES was reduced without statistical significance.Conclusions: The results base on this study showed a significant increase in the trunk ROM after trunk flexion and extension isometric exercise using an external compression device, while the relative contraction onset timing in the GM was significantly reduced. Furthermore, the muscle endurance test after exercise showed a trend of increase in the duration time with a decreasing trend in muscle fatigue in the ES. Exercise-based on ATM®2 is an effective exercise protocol with an effect on the biomechanics of healthy subjects.· Clinical trial registration numbers : KCT0006728· Clinical trial registration date : 09/11/2021
Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolites
Authors: Sun Lijuan , Wang Xueying , Chen Yue , Song Jianxia , Liang Changting , Zhang Jianbin 
Published: May 2022

Abstract
Objective
The westernized lifestyle has been paralleled by an epidemic of inflammatory bowel disease (IBD). Excessive consumption of cold beverages is especially common among the modern humans. However, whether cold stress contributes directly to the gut barrier and gut-brain axis is not clear. We investigated whether the cold stress affect gut inflammation or not.
Material
: 16 male mice were used in this study.
Treatment:
The mice were treated with 14 consecutive days of intragastric cold or common water administration.
Methods
We observed changes in gut transit and gut barrier in the colon.We also employed RNA sequencing-based transcriptomic analysis to identify the genes potentially driving gut injury, and simultaneously examined the gut microbiota and metabolites in the feces.
Results
Cold stress inhibited the colonic motility and increased gut permeability. A set of core genes related to immune responses were consistently overexpressed in the cold stress group. Additionally, cold stress induced decreased bacterial diversity, ecological network, and increased pathogens mainly belonging to Proteobacteria. The dopamine signaling pathway-related metabolites were largely reduced in the cold stress group.
Conclusion
This study revealed that cold stress could trigger an IBD-like phenotype in mice, implying that cold stress is a possible risk factor for IBD development.
Inverse association between fasting blood glucose and occurrence of gallbladder cancer in type 2 diabetes mellitus patients: a case-control study
Authors: Sheng Bin-Wu , Zhang Jianqin , Chen Min , Ma Mao 
Published: May 2022

Abstract
Purpose To explore the reason for the correlation between diabetes mellitus (DM) and gallbladder cancer (GBC) in an epidemiological setting. Methods We summarized the clinical and laboratory data of 2210 GBC patients within 3524 Chinese patients in our hospital from January 2009 to December 2020. Seventeen influencing factors for GBC, including gender, body mass index (BMI), fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR), retinol binding protein (RBP), lipid indexes, and others, were analyzed by unconditional logistic regression analysis in this case-control study. Results On univariate logistic regression, GBC likelihood was significantly and positively correlated with serum triglyceride, low-density lipoprotein cholesterol, FINS, and HOMA-IR; being female; BMI; DM; NAFLD; and GSD, and it was significantly negatively correlated with high-density lipoprotein cholesterol and FBG concentrations in serum and with hypertension. In multivariate analysis, FINS was significantly positively associated with GBC risk, while DM had an insignificant negative association, and FBG levels were also not important. HOMA-IR was a most remarkable independent factor of GBC risk in DM patients. FBG levels showed a significant negative relationship to GBC in DM individuals. Conclusions Our findings revealed that the efficient treatment of insulin-resistance is an important approach for decreasing GBC risk, as opposed to lowering blood sugar alone, especially in DM patients. Interestingly, FBG might have an inverse association with the development of GBC in type 2 DM patients. Exciting, we found initially that a dramatic drop in RBP might be helpful in predicting the occurrence of GBC.
SSR mining of black cumin (Nigella sativa L) transcriptome for molecular markers development
Authors: Celik Ibrahim , AYDIN Adnan 
Published: May 2022

Abstract
Black cumin (Nigella sativa L.) is an important aromatic plant due to the production of many health-beneficial metabolites. Up to date- molecular characterizations of the germplasm were performed by using general marker systems. In the present study, the transcriptome of the black cumin was searched for SSRs. Dinucleotide and trinucleotide repeats were the major repeat types which comprised 52.4% and 44.7% of total SSRs, respectively. TC/GA was the most frequent repeat motif (11.8%). A total of 4,779 SSR markers were developed. Functional annotation of 2,456 SSR markers was performed. For validation of the SSR markers, randomly selected 20 markers were tested in a subset population containing nine Nigella sativa and one Nigella damascena genotypes. A total of 13 SSR makers produced clear amplification and generated 41 polymorphic fragments with mean gene diversity (0.25). Principal Coordinate Analyses (PCoA) and MCMC methods based on hierarchical clustering analysis revealed two clusters and moderate diversity with a mean value of 0.66 and 0.57 for interspecific and intraspecific populations, respectively. This is the first report of the development of SSR markers from black cumin transcriptome. These high-throughput crop-specific SSR markers are valuable genomic sources for comprehensive genome analysis in black cumin.
Upregulated ONECUT2 impeded the efficacy of 5-FU treatment to relapsed patients and might be an emerging indicator of chemotherapy in gastric cancer
Authors: Zhu Haifeng , Chen Shu , Long Weiguo , Wang Xu 
Published: May 2022

Abstract
Background: Chemotherapeutic resistance is a main obstacle for prolonging the lifetime of gastric cancer (GC) patients, so new emerging solutions still remain to be investigated. We aim to identify an effective target, ONECTU2, for chemotherapy of GC patient, particularly relapsed patients.Methods: STAD patients matched with clinical features and transcriptome sequencing data from TCGA were used in this study. Gene set enrichment analyses (GSEA) were performed using 100 patients who were divided average according to ONECUT2 level. Cytotoxic assays for 5-FU and cisplatin were used to test the role of chemotherapeutic resistance of ONECUT2 in GC cells. 30 relapsed patients with previously resected tumor samples were collected to validate the efficacy of ONECUT2 in GC chemotherapy.Results: ONECUT2 was a crucial regulator of Disease-Free Interval (DFI) in GC patients with reoccurrence once having chemotherapeutic treatment. Gene set enrichment analysis (GSEA) results suggest that upregulated ONECUT2 is positively correlated with the increased ability of xenobiotic drug metabolism, which might contribute to the pharmaceutical resistance in GC. Targeting ONECUT2 reduced half-maximal inhibitory concentration (IC50) of 5-FU and cisplatin in GC cells. The efficacy of 5-FU treatment was negatively correlated with ONECUT2 mRNA level of tumor samples derived from GC patients who relapsed after surgical resection.Conclusions: These findings indicate that ONECUT2 displays a drug-resistant function in GC, providing a new therapeutic target for GC treatment.
Droplet microfluidics based combinatorial indexing for massive-scale 5′-end single-cell RNA sequencing
Authors: Jiang Lan , Li Yun , Huang Zheng , Zhang Zhaojun , Wang Qifei , Fang Xiangdong , Wang Shufang , Li Fengxian 
Published: May 2022

Abstract
Single-cell RNA sequencing methods focus on 5’-end of transcripts can reveal promoter and enhancer activity and allows efficiently profiling immune receptor repertoire. However, ultra-high-throughput 5′-end single-cell RNA sequencing methods have not been described. Here, we introduce five-prime end single-cell combinatorial indexing RNA-Seq (FIPRESCI), enabling the massive sample multiplexing and increasing the throughput of the droplet-microfluidics system by over 10-fold. FIPRESCI is based on combining the ability of indexed Tn5 transposome to barcoding RNA/cDNA hybrids heteroduplexes in situ and Template Switching Oligo barcoding of commercial droplet-microfluidic. Using FIPRESCI, we profiled transcriptome and transcribed cis-regulatory elements from various human and mouse cell lines and demonstrated the approach is compatible for both cells and nuclei. We applied FIPRESCI to E10.5 whole mouse embyro and uncover previously unknown isoform switch during GABAergic neurogenesis of several important regulators, including Rbfox2. We further applied FIPRESCI to primary T cells from human peripheral blood mononuclear cells (PBMCs) and demonstrated it enable simultaneous identification of cancer specific gene expression and T cell receptor (TCR) signatures. Given its simplicity, flexibility, and scalability, FIPRESCI will have wide application in cell atlas projects, large-scale screening, and single-cell immune profiling of large cohorts’s studies.
Invading bivalves replaced the native Levantine populations with negligible local effects on the benthic community
Authors: Diga Rei , Gilboa Merav , Moskovich Raz , Darmon Neomie , Amit Tal , Belmaker Jonathan , Yahel Gitai 
Published: May 2022

Abstract
Constructing the Suez Canal connected the Red Sea and the Mediterranean Sea, allowing rapid marine bio-invasion. Over the last century, several bivalve species have invaded the Levantine basin, yet their distribution and impact on the benthic community have not been thoroughly studied. Large-scale benthic surveys along the rocky substrate of the Israeli Mediterranean coastline indicate that invading bivalves now dominate the rocky environment, reaching densities of tens to hundreds of individuals per m2. No native bivalve specimens were found in any of the transects surveyed. The small-scale ecological effects of the established invading populations on the benthic community were examined using in-situ exclusion experiment where all invading bivalves were either physically removed or poisoned and kept in place to maintain the physical effect of the shells. Surprisingly, the experimental exclusion showed little measurable effect of bivalve presence on the invertebrate community in close vicinity (~1 m). Bivalves presence had a small, but statistically significant, effect only on the community composition of macroalgae, increasing the abundance of some filamentous macroalgae, and reducing the cover of turf. The generally low impact of invading bivalves could be due to (1) wave activity and local currents dispersing the bivalve excreta, (2) high grazing pressure, possibly by invading herbivorous fish, reducing the bottom-up effect of increased nutrient input by the bivalves, or (3) the natural complexity of the rocky habitat masking the contribution of the increased complexity associated with the bivalve’s shell. We found that established invading bivalves have replaced native bivalve species, yet their small-scale effects on the benthic community seem to be negligible.
Small and large scale engineering design optimization using an innovative hybrid algorithm
Authors: Parouha Raghav Prasad 
Published: May 2022

Abstract
An innovative hybrid algorithm (ihPSODE) present in this paper, for finding the solution of small and large scale engineering design optimization problems. A new particle swarm optimization (nPSO) and differential evolution (nDE) introduced in ihPSODE, to promote the search accuracy. Besides, a novel inertia weight, acceleration factor and position update structure is adopted in nPSO to increase the population diversity as well as a novel mutation approach and crossover rate is implemented in nDE to escape from local optima. After fitness function calculations recognize the top half member with discard rest half and apply nPSO which help to sustain exploration and exploitation competency of the algorithm. Furthermore, to achieve rapid convergence and stability, apply nDE on offspring created by nPSO. The population resultant by nPSO and nDE are combined for repetition. The efficiency of ihPSODE with its integrating nPSO and nDE algorithm validated on 23 unconstrained benchmark functions and utilized to solve 5 small (structural optimization) and 1large (economic dispatch with 3-, 6-, 15-, 40-, 140-unit test system considering with or without valve point effect) scale engineering design optimization problems plus comparing the results with various modern algorithms. Experimental and equated results confirm the superiority of the proposed algorithms.
Evaluation of Gentamicin Dosage Regimen and Route of Administration Based on Achieved Serum Concentrations in Neonates
Authors: Lim Chuan Poh , Candra Samuel Rocky , Edison Ebenezer Priyantha , Tan Mary Grace , Yong Mei Hui Amanda , Chen Yufei , Yeo Cheo Lian 
Published: May 2022

Abstract
Purpose
Combination antibiotics consisting beta-lactam and aminoglycoside are commonly utilized in the treatment of neonatal septicaemia. The aims of this study were (1) to determine safety and efficacy of the revised gentamicin dosage regimen and (2) to compare intravascular (IV) versus intramuscular (IM) route of gentamicin administration, in term of attaining peak and trough serum concentration targets.
Methods
This was a retrospective study from 2012 to 2017. All neonates who received gentamicin with therapeutic drug monitoring performed were included in this study. Data for all eligible neonates were collected from electronic medical records and included demographics, serum creatinine levels, the complete gentamicin dosing and concentration history.
Results
A total of 737 neonates who received the correct institutional gentamicin dosage regimen (± 5% of the intended dose) were included in this study. There were 635 trough concentrations (within 30 minutes prior to subsequent dose) and 430 peak concentrations (60 to 90 minutes post administration) included in the comparison of IV and IM route of administration. With the revised dosage regimen, 91% of peak and 98% of trough concentrations were found within therapeutic range. There was no difference in terms of proportion of trough concentrations within therapeutic range for IV as compared to IM (97.8 vs 98.6%, p = 0.556) while IM resulted in a higher proportion of peak concentrations within therapeutic range (86.1 vs 97.0%, p < 0.001).
Conclusions
The current institutional gentamicin dosage regimen is safe and effective in attaining therapeutic targets. Intramuscular injection can be an alternative route of administration for gentamicin in neonates.
Determinants of Nonrenewal of National Health Insurance (NHI) Membership Cards Among Healthcare Workers in the Kintampo North Municipality, Bono East Region of Ghana.
Authors: Hallidu Mustapha , Sumaila Issah 
Published: May 2022

Abstract
Introduction:
National Health Insurance Scheme (NHIS) was implemented in Ghana in 2004 to serve as the lifeline to realizing Universal Health Coverage (UHC). Available evidence suggests that, formal sector workers do not promptly renew their expired NHIS membership cards. This study was therefore conducted to unearth factors responsible for the failure of healthcare workers in the Kintampo North Municipality to promptly renew their health insurance membership whenever it expires.
Methods
A descriptive cross-sectional approach was used to conduct this study where three hundred and ninety-seven (397) participants were recruited using a proportionate stratified sampling technique. All variables with a p-value < 0.25 at the bivariate analysis level were selected and put into multiple logistic regression analysis models for statistical significance (p-value < 0.05). Odds ratios with their corresponding 95% Confidence Interval were reported. A p-value < 0.05 was set as level of significance.
Results
Almost all the respondents (94.0%) had NHIS membership cards; out of which 70.7% had valid membership cards. 40.0% did not renew their expired NHIS membership cards for more than 7 months. From the study, reasons given for health workers’ inability to promptly renew NHIS membership included: 212 (19.8%) indicated forgetfulness, busy schedules 191 (17.9%), procrastination 167 (15.6%), self-medication 170 (15.9%) and utilization of spiritual homes (4.5%). All socio-economic factors were significantly associated at the bivariate level (p < 0.05). However, in the multiple logistic regression model, employment status, the type of health staff and monthly salary lost their statistical significance.
Conclusion
NHIS subscription and membership renewals is high among healthcare works in the Kintampo North District of the Bono East Region of Ghana. However, there is the need to encourage those who do not renew their expired cards by ensuring that more NHIS cards renewal centers are established at close proximity to the health facilities. It will be prudent for NHIS to liaise with Government of Ghana (GoG) to put measures in place to facilitate automatic membership renewals for public sector workers who for some other reasons often fail to renew their cards.