bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differently Regulated Gene-Specific Activity of Enhancers Located at the Boundary of Subtopologically Associated Domains: TCRα Enhancer
Authors: Rodríguez-Caparrós Alonso , Álvarez-Santiago Jesús , López-Castellanos Laura , Ruiz-Rodríguez Candela , Valle-Pastor María Jesús , López-Ros Jennifer , Angulo Úrsula , Andrés-León Eduardo , Suñé Carlos , Hernández-Munain Cristina 
Published: January 2022

Abstract
Enhancers activate transcription through long-distance interactions with their cognate promoters within a particular subtopologically associated domain (sub-TAD). The TCRα enhancer (Eα) is located at the sub-TAD boundary between the TCRα and DAD1 genes and regulates transcription toward both sides in an ∼1-Mb region. Analysis of Eα activity in transcribing the unrearranged TCRα gene at the 5′-sub-TAD has defined Eα as inactive in CD4−CD8− thymocytes, active in CD4+CD8+ thymocytes, and strongly downregulated in CD4+ and CD8+ thymocytes and αβ T lymphocytes. Despite its strongly reduced activity, Eα is still required for high TCRα transcription and expression of TCRαβ in mouse and human T lymphocytes, requiring collaboration with distant sequences for such functions. Because VαJα rearrangements in T lymphocytes do not induce novel long-range interactions between Eα and other genomic regions that remain in cis after recombination, strong Eα connectivity with the 3′-sub-TAD might prevent reduced transcription of the rearranged TCRα gene. Our analyses of transcriptional enhancer dependence during T cell development and non-T lineage tissues at the 3′-sub-TAD revealed that Eα can activate the transcription of specific genes, even when it is inactive to transcribe the TCRα gene at the 5′-sub-TAD. Hence distinct requirements for Eα function are necessary at specific genes at both sub-TADs, implying that enhancers do not merely function as chromatin loop anchors that nucleate the formation of factor condensates to increase gene transcription initiated at their cognate promoters. The observed different regulated Eα activity for activating specific genes at its flanking sub-TADs may be a general feature for enhancers located at sub-TAD boundaries.
Pengaruh Konflik Peran dan Jenjang Karir terhadap Produktivitas Karyawan dengan Komitmen Organisasional sebagai Variabel Mediasi di RS Leona Kupang
Authors: Mitzi Mitzi , Fachruddin Arrozi Muhammad , Anindita Rina 
Published: January 2022

Produktivitas Karyawan adalah sebuah isu yang sangat penting dalam dunia kerja terutama di industri Rumah Sakit. Hal ini dikarenakan dalam sebuah rumah sakit, salah satu hal yang dijual adalah jasa. Jasa ini pasti erat kaitannya dengan sumber daya manusia dan komitmen dia produktivitasnya dalam bekerja. Banyak faktor yang dapat mempengaruhinya, baik faktor internal dan eksternal. Penelitian ini bertujuan untuk melihat Pengaruh Konflik Peran dan Jenjang Karir terhadap Produktivitas Karyawan dengan komitmen organisasional sebagai variabel mediasi. Teknik analisa yang dipergunakan path analysis. Populasi sampel penelitian ini adalah seluruh staf di RS Leona Kupang berjumlah 158 orang. Teknik pengambilan sampel menggunakan Kuota Sampling dengan desain penelitian cross-sectional. Pengukuran dalam penelitian ini menggunakan kuesioner yang disebar kepada 158 responden dan kuesioner yang kembali sebanyak 100%. Hasil penelitian menunjukkan bahwa konflik peran, jenjang karir dan komitmen organisasional memiliki pengaruh signifikan terhadap produktivitas karyawan. Namun temuan pada penelitian ini bahwa konflik peran berpengaruh negatif terhadap produktivitas karyawan sedangkan jenjang karir dan komitmen organisasional juga berpengaruh positif terhadap produktivitas karyawan. Implikasi penelitian untuk mendorong manajemen mengembangkan strategi untuk meningkatkan produktivitas karyawan
Profil Penggunaan Obat Hipertensi pada Pasien BPJS Rawat Jalan di Instalasi Farmasi di Rumah Sakit X Sukabumi
Authors: Ismaya Wisma , Emelia Rida 
Published: January 2022

Telah dilakukan penelitian Profil Penggunaan Obat Hipertensi Pada Pasien BPJS Rawat Jalan di Instalasi Farmasi di Rumah Sakit x sukabumi jangka waktu 5 april s/d 31 mei 2021. Latar belakang dari penelitian ini adalah meningkatnya prevalensi penderita penyakit kronis dibanding penyakit non kronis dalam penelitian ini penyakit kronis dikhususkan pada hipertensi. Adapun tujuan penelitian ini untuk mendapatkan gambaran tentang profil penggunaan obat anti hipertensi pada pasien rawat jalan yang menggunakan BPJS dengan metode penelitian yang digunakan deskriptif retrospektif yaitu dengan mengambil data sekunder yang bersumber dari resep pasien rawat jalan BPJS pada waktu yang telah ditentukan dikhususkan yang menderita hipertensi dan dikelompokan berdasarkan jenis kelamin, usia, golongan obat, jenis obat dan jumlah obat pada resep. Pasien rawat jalan BPJS yang menderita hipertensi terbanyak adalah laki - laki yaitu sebanyak 198 orang (53,37 %), usia penderita adalah diatas 55 tahun (70.35%), golongan anti hipertensi yang paling banyak digunakan adalah penghambat ACE (42,24%) dan yang paling sedikit adalah nitrat (0,9%), jenis anti hipertensi yang paling banyak diresepkan adalah amlodipine yaitu sebanyak 147 item (33,03%) dan yang paling sedikit adalah diltiazem yaitu sebanyak 3 item (0,67%), jumlah item anti hipertensi yang paling banyak diresepkan adalah satu item antihipertensi (82,21%).
A VONTADE GERAL E O PAPEL DO LEGISLADOR EM ROUSSEAU
Authors: Zimmermann Jr Giovani Luiz 
Published: January 2022

O autor genebrino em seu ideal visava construir o novo homem político, que daria origem a uma nova política, capaz de substituir os fundamentos injustos sobre os quais a sociedade foi construída. Para que tudo isso mude é necessário um novo pacto baseado na vontade geral, a qual por sua vez não simplesmente é a soma das vontades particulares uma a uma, mas tão somente retém o suprassumo, isto é, a essência de todas as vontades unidas numa só, cujo maior valor é a vontade coletiva (moral coletiva). Para que haja uma sociedade justa, é necessária a soberania do povo através da vontade geral, sem poderosos e oprimidos, livres ou escravos. Todos os contratantes devem poder agir, sobretudo, com igualdade e liberdade. Essa nova sociedade é aquela que ainda está por ser alcançada. Já não é possível, conservar a condição do estado natural. A desnaturação gesta um novo homem, que passa a viver com os outros e, nessa nova condição, sofrerá mudanças, virtualmente possíveis em seu estado. Na base dessas mudanças está a necessidade primária de criação de um artifício, o “espírito social”, assentado sobre uma condição existencial básica. Que condição é essa? É a condição de homem ao mesmo tempo “integrado” (súdito) e “integrante” (cidadão). Buscando garantir a legitimidade da vida social, Rousseau preserva dois valores fundamentais: a liberdade e a igualdade. E pensa ter encontrado a fórmula para o homem que se associa, uma condição que, a seu ver, lhe daria um poder soberano, necessário à salvaguarda desses valores.
Pengetahuan Ibu hamil Tentang Pencegahan COVID-19
Authors: El Rahman Lubna Tahta Lazuardi , Khasanah Yuni Uswatun , Ratnawati Anggit Eka 
Published: January 2022

Latar Belakang: Menurut data dari Perkumpulan Obstetri dan Ginekologi Indonesia (POGI) Jakarta, didapatkan jumlah ibu hamil yang terinfeksi COVID-19 sejumlah 13,7% ibu hamil. Hal ini dikarenakan wanita hamil memiliki riwayat perjalanan atau tinggal di wilayah transmisi lokal, riwayat kontak dengan kasus konfirmasi, dan riwayat kontak dengan hewan yang terinfeksi COVID Metode: Desain yang digunakan adalah deskriptif kuantitatif dengan pendekatan cross sectional. Populasi dalam penelitian ini adalah seluruh ibu hamil yang periksa di PMB Sumarni Pundong Bantul sebanyak 30 responden. Pengambilan data menggunakan kuesioner sebanyak 27 kuesioner yaitu 5 soal dari uji validitas dan 22 soal menggunakan kuesioner yang diadopsi dari penelitian Yanti, dkk (2020), sedangkan analisa menggunakan analisa univariat. Hasil: Hasil penelitian ini menunjukkan bahwa mayoritas responden berusia 20-35 tahun sebanyak 27 responden (90%), berpendidikan SMA sebanyak 16 reponden (53%), Bekerja sebanyak 19 responden (63,3%), Multipara sebanyak 18 responden (60%), trimester 3 sebanyak 18 responden (60%) responden berpengetahuan baik sebanyak 22 responden (73,3%), dan pengetahuan cukup sebanyak 8 responden (26,7%). Kesimpulan: Pengetahuan ibu Hamil di PMB Sumarni Pundong Bantul Yogyakarta tahun 2021 adalah Baik.
Rancang Bangun Robot Pemindah Barang Berbasis Mikrokontroler Untuk Monitoring Stok Gudang
Authors: Bima Jodhi Satria  , Sumaryanto  
Published: January 2022

This study generally aims to design an information system that can handle problems in putting and sorting goods into warehouses at CV. Sekar Intan KIC Gatsu, Semarang. In terms of using the Information System, CV. Sekar Intan KIC Gatsu, Semarang. In the transfer and sort of goods in the warehouse still using a manual system when the implementation, warehouse employees must take and place the goods to the warehouse that has been determined each - each type of item, so employees must calculate the number of items when the goods take place, here various problems occur, because it requires some HR to manage the warehouse performance process at CV. Sekar Intan.
To support the research, researchers used Research and Development (R & D) research methods through the microcontroller-based moving robot design simulation design stages that have been tested by experts declared valid with Valid validity levels and final product development design simulation prototypes carried out. goods transfer robot uses Arduino IDE development software that is integrated with Arduino Mega 2560, 6 pin line sensor, dc motor, motor driver, servo motor, motor driver, lcd, and has been tested in the field by General Adv. Administration and HRD as users / users and stated to be very effective.
Based on the results of the research and discussion, it can be concluded that the robot design system developed can overcome the problems in the old system on the CV. Sekar Intan KIC Gatsu Semarang, so as to produce a system that can facilitate work processes and parking processes for employees to be more effective and efficient.
SISTEM PAKAR DIAGNOSA KERUSAKAN MOTOR YAMAHA NMAX MENGGUNAKAN METODE FORWARD CHAINING
Authors: Wiwid Wahyudi  , Khoirur Rozikin  , Afriliawan Indra Permana  
Published: January 2022

The recruitment of new mechanics at Mataram Sakti is no longer through the training process at the head office, but new mechanics are placed at the dealership accompanied by the chief mechanic as a supervisor, with the aim of providing training to new mechanics in order to be able to service or periodically repair Yamaha products especially the latest motorbikes namely Yamaha NMAX, because of the flurry sometimes the mechanical head is not in place so that when the mechanic is experiencing problems it cannot directly ask the mechanical head causing the handling of repairs to be less effective and efficient.
One way to overcome this is to create a web-based expert system application with a forward chaining method so that the chief mechanic can transfer the knowledge he has into the computer. Forward chaining method is a search technique that starts with known facts and then matches these facts with the IF part of the IF-Then rules, so that conclusions can be drawn. This expert system was built using supporting software including, Macromedia Dreamweaver MX, PHP script language, MySQL database and WAMP server.
The expert system for diagnosing damage to Yamaha NMAX motorcycles using the forward chaining method can be used as a new mechanical learning media in diagnosing damage to NMAX motorcycles and as a means of chief mechanics transferring their knowledge.
Aquifer recharge in the Piedmont Alpine zone: historical trends and future scenarios
Authors: Brussolo Elisa , Palazzi Elisa , von Hardenberg Jost , Masetti Giulio , Vivaldo Gianna , Previati Maurizio , Canone Davide , Gisolo Davide , Bevilacqua Ivan , Provenzale Antonello , Ferraris Stefano 
Published: January 2022

Abstract. The spatial and temporal variability of air temperature, precipitation, actual evapotranspiration (AET) and their related water balance components, as well as their responses to anthropogenic climate change, provide fundamental information for an effective management of water resources and for a proactive involvement of users and stakeholders, in order to develop and apply adaptation and mitigation strategies at the local level. In this study, using an interdisciplinary research approach tailored to water management needs, we evaluate the past, present and future quantity of water potentially available for drinking supply in the water catchments feeding the about 2.3 million inhabitants of the Turin metropolitan area (the former Province of Turin, north-western Italy), considering climatologies at the quarterly and yearly timescales.
Observed daily maximum surface air temperature and precipitation data from 1959 to 2017 were analysed to assess historical trends, their significance and the possible cross-correlations between the water balance components.
Regional climate model (RCM) simulations from a small ensemble were analysed to provide mid-century projections of the difference between precipitation and AET for the area of interest in the future CMIP5 scenarios RCP4.5 (stabilization) and RCP8.5 (business as usual). Temporal and spatial variations in recharge were approximated with variations of drainage. The impact of irrigation, and of snowpack variability, on the latter was also assessed. The other terms of water balance were disregarded because they are affected by higher uncertainty. The analysis over the historical period indicated that the driest area of the study region displayed significant negative annual (and spring) trends of both precipitation and drainage. Results from field experiments were used to model irrigation,
and we found that relatively wetter watersheds in the northern and in the southern parts behave differently, with a significant increase of AET due to irrigation.
The analysis of future projections suggested almost stationary conditions for annual data. Regarding quarterly data, a slight decrease in summer drainage was found in three out of five models in both emission scenarios. The RCM ensemble exhibits a large spread in the representation of the future drainage trends.
The large interannual variability of precipitation was also quantified and identified as a relevant risk factor for water management, expected to play a major role also in future decades.
Determinants of voter participation in Latin American referendums
Authors: Strnad Matyáš 
Published: January 2022

Voter turnout in regular parliamentary or presidential elections is a very frequent topic, mainly in the recent debate about its decline. This article works with several theories explaining variations in voter turnout that are subsequently applied on referendums in Latin America. Referendums as one of the main pillars of direct democracy are not, in this regard, sufficiently scientifically explored topic. In this point of view, the region of Latin America is an ideal environment for research of the given subject, because there are many cases of direct democracy applications in the institutional configuration as well as in practice. Voter turnout in referendums is set into a comparative perspective using regression models that allow the researcher to monitor possible correlations and control explaining values. The analysis employs aggregate data from various statistical databases (e. g. V-Dem, Polity IV, World Bank) as well as secondary data from available comparative literature focused on elections (and referendums) in Latin America. The results indicate very high circumstantiality of referendums and the respective societies that apply them in politics. Some institutional settings have been found to positively influence the turnout, mainly compulsory elections.
Evaluation of the techniques and steps of intranasal corticosteroid sprays administration
Authors: Rattanawong Supachet , Wongwattana Panuwat , Kantukiti Supatat 
Published: January 2022

Background
Intranasal corticosteroid (INCS) is one of the most effective treatments for allergic rhinitis (AR). To achieve optimum efficiency while avoiding adverse effects (AEs), patients must comply with and follow the drug administration instructions.

Objective
To evaluate INCS administration techniques and steps and the association between inaccurate drug administration and AEs.

Methods
A descriptive cross-sectional study was performed in patients diagnosed with AR who had used an INCS for more than 1 month at the HRH Princess Maha Chakri Sirindhorn Medical Center, Nakhon Nayok, Thailand between September 2020 and August 2021. Patient information was collected, evaluate the accuracy of the application techniques and the steps they followed for INCS administration and the occurrence of any associated AEs.

Results
In total, 150 subjects diagnosed with AR met the criteria. Only 6 patients (4%) correctly performed all 12 steps of INCS administration, while 44 patients (29.33%) correctly performed the 5 recommended essential steps. AEs were found in 23 patients (15.33%), with not pointing the tip slightly outward away from the septum significantly associated with a 3.6 times higher risk of AEs in patients (odds ratio, 3.6; 95% confidence interval, 1.3-9.4; p = 0.012).

Conclusion
Investigations into INCS use in patients with AR revealed that only 4% of patients correctly performed all the administration techniques and steps, while 30% of patients at least followed the 5 recommended essential steps perfectly. Not pointing the tip slightly outward away from the septum was found to result in the most AEs.

Trial Registration
Thai Clinical Trials Registry: TCTR-20210718003
Effect of Lamun Leaves [Enhalus acoroides (L.f.) Royle] on Leydig Cell Morphology
Authors: Antarsih Novita Rina , Hardiyanto Lutfi , Jusman Sri Widia A. 
Published: January 2022

The ageing male process lead deterioration fertilization ability that caused of Leydig cell degeneration by an accumulation of oxidative stress with decreased testosterone levels. Enhalus acoroides contains antioxidants and phytosterols which can be converted into testosterone. The aim of this study to investigate the extract of Enhalus acoroides effect on Leydig cell morphology in male mice. Male mice was divided into 8 groups: adult mice no treatment (AG1), adult mice with olive oil (AG2), adult mice with extract of Enhalus acoroides at dose 25 mgKg-1 (AG3), adult mice with extract of Enhalus acoroides at dose 50 mgKg-1 (AG4), old mice no treatment (OG1), old mice with olive oil (OG2), old mice with extract of Enhalus acoroides at dose 25 mg Kg-1 (OG3), and at dose 50 mgKg-1 (OG4). All groups were repeated for 14 days. There was a significantly increased number of old Leydig cells in old mice group and adult mice group at dose 25 mgKg-1 compared without treatment. There is no difference in the observation of adult Leydig cell degeneration.
Corpus-Based Error Analysis of Chinese Learners’ Use of High-Frequency Verb Take
Authors: Li Yanru 
Published: January 2022

This study investigated the erroneous use of the high-frequency verb TAKE by the Chinese college learners of English as a foreign language (EFL), aiming to identify the similarities and differences between Chinese EFL learners, aimed at finding out more effective ways for the teaching and researching of the high-frequency verbs. Corpus-based Contrastive Interlanguage Analysis and Error Analysis were carried out in the present study, with the subcorpora ST4 and ST6 of CLEC (Chinese Learner English Corpus) as the learner corpora.&nbsp;
The analyses involved the misuse of the verb TAKE by the Chinese EFL learners. The error analysis of TAKE was based on the classification in the corpus CLEC. From the perspective of the overall frequency, the ST6 learners commit fewer errors than the ST4 learners. From the perspective of error types, the ST6 learners and the ST4 learners have much in common. That is, the error types of &ldquo;wd&rdquo; and &ldquo;cc&rdquo; take up an overwhelming part of all the errors in both corpora. These errors are caused by some interlingual and intralingual factors such as language transfer, overgeneralization, and communication strategy. In comparison, in the process of EFL learning, the non-English majors are interfered by their mother tongue to a larger extend than the English majors.
Modeling and synthesis of carbon-coated LiMnPO4 cathode material: Experimental investigation and optimization using response surface methodology
Authors: El-Khalfaouy Redouan , Khallouk Khadija , Elabed Alae , Addaou Abdellah , Laajeb Ali , Lahsini Ahmed 
Published: January 2022

Nanostructured LiMnPO4 cathode materials for lithium-ion batteries (LIBs) have been successfully prepared by a modified solvothermal method under controlled conditions. Polyethylene glycol (PEG-10000) was used as a solvent to optimize the particle size/mor­phology and as a carbon conductive matrix. In order to investigate the effect of synthesis parameters such as concentration of PEG-10000, reaction time and reaction temperature on the LiMnPO4 phase purity, Response surface methodology was carried out to find variations in purity results across the composition. The purity of all materials was checked using HighScore software by comparing the matched lines score to ones of reference data. As a result, it has been found that the pure phospho-olivine material LiMnPO4 can be syn­thesized using the following optimum conditions: PEG concentration = 0.1 mol l-1, reaction time = 180 min, and reaction temperature = 250 °C. The as-prepared LiMnPO4 under opti­mum conditions delivered an initial discharge capacity of 128.8 mAh g-1 at 0.05 C‑rate. The present work provides insights and suggestions for optimizing synthesis conditions of this material, which has been considered the next promising cathode candidate for high-energy lithium-ion batteries.
Training as enrichment: A critical review
Authors: Fernandez EJ 
Published: January 2022

AbstractHusbandry training and environmental enrichment are both important advancements associated with current behavioural welfare practices. Additionally, the use of training procedures has been proposed as a form of enrichment, with the implication that training can produce beneficial behavioural welfare results. This paper examines the concept of training as enrichment through three distinct ways training procedures could enrich: (i) training facilitates enrichment usage; (ii) training modifies interactions, conspecific or otherwise; and (iii) training expands behavioural repertoires. Within each category, the paper focuses on past research that provides empirical support for training functioning as enrichment, as well as related areas of research that provide additional evidence. Previous studies support the claim that training is enriching, with additional research necessary to better understand how prevalent and under what conditions training procedures function as enrichment. Future training research should examine these potential enrichment effects, including methodology that allows for comparisons to traditional enrichment, the use of welfare diversity/variability indices, and the effects of learning on trainers and trainees alike.