bluuebunny/crossref_metadata_2025_split huggingface dataset:
Micro-recycling of spent Ni-Cd batteries for the obtention of electrocatalytic coatings
Authors: Delvasto Pedro , Rueda Héctor , Monsalve Andrés , Vargas Ronald , Blanco Sergio , Delvasto Pedro 
Published: January 2022

Abstract
A micro-recycling approach was explored to produce catalytic metallic coatings for the hydrogen evolution reaction (HER). For this aim, discarded Ni-Cd batteries were employed as raw material. After dismantling the batteries, the active powder material, containing Cd and Ni compounds, was leached in a solution containing citric acid and hydrogen peroxide. The dissolved metals were electro-deposited on copper plates using a two electrodes cell at the following potentials (mV): -1900, -2000, and -2100 mV. The CdNi coating produced at -2000 mV, contained 92.6 % Cd and 7.4 % Ni. This coating was studied by cyclic voltammetry (CV) and potentiodynamic analysis in two different KOH solutions (0.1 M and 1.0 M). The CV analysis showed that the CdNi electrode was electrochemically stable in a wide operating voltage range (between oxygen evolution reaction and HER). Using an uncompensated resistance correction, the Tafel slopes for HER were obtained. The potentiodynamic analysis revealed that the synthesized CdNi electrode showed a catalytic activity for HER just 25.5 % smaller than the correspondent response of a standard pure Ni electrode. Our results serve as a proof of concept about the application of micro-recycling of spent batteries to produce sustainable electroactive catalytic materials for hydrogen production.
A Preclinical Model of Cutaneous Melanoma Based on Reconstructed Human Epidermis
Authors: Groeber-Becker Florian K. , Leikeim Anna , Wußmann Maximiliane , Schmidt Freia F. , Neto Nuno G. B. , Benz Franziska , Tiltmann Kendra , Junger Corinna , Monaghan Michael G. , Schilling Bastian 
Published: January 2022

Abstract
Malignant melanoma is among the tumor entities with the highest increase of incidence worldwide. To elucidate melanoma progression and develop new effective therapies, rodent models are commonly used. While these do not adequately reflect human physiology, two-dimensional cell cultures lack crucial elements of the tumor microenvironment. To address this shortcoming, we have developed a melanoma skin equivalent based on an open-source epidermal model. Melanoma cell lines with different driver mutations were incorporated into these models forming distinguishable tumor aggregates within a stratified epidermis. Although barrier properties of the skin equivalents were not affected by incorporation of melanoma cells, their presence resulted in a higher metabolic activity indicated by an increased glucose consumption. Furthermore, we re-isolated single cells from the models to characterize the proliferation state within the respective model. The applicability of our model for tumor therapeutics was demonstrated by treatment with a commonly used v-raf murine sarcoma viral oncogene homolog B (BRAF) inhibitor vemurafenib. This selective BRAF inhibitor successfully reduced tumor growth in the models harboring BRAF-mutated melanoma cells. Hence, our model is a promising tool to investigate melanoma development and as a preclinical model for drug discovery.
Antibacterial Efficacy of Herbal Toothpaste Containing Bamboo Salt: A Randomized Controlled Clinical Trial
Authors: Biria Mina , Rezvani Yasaman , Roodgarian Romina , Rabbani Abbas , Iranparvar Parastoo 
Published: January 2022

Abstract
Background: The inclusion of herbal antibacterial agents in the composition of toothpastes is becoming increasingly popular, due to lower side effects. The present study intended to investigate the antibacterial efficacy of a herbal toothpaste containing Bamboo salt on cariogenic oral bacteria. Methods: The present double-blinded parallel randomized controlled clinical trial was conducted on 60 dental students (age range: 18-30). Following the baseline saliva sampling, the participants were randomly assigned into the case and control groups, to use the Bamboo salt herbal toothpaste and conventional non-herbal toothpaste, respectively. They were instructed to brush their teeth twice a day using the Bass technique. Saliva sampling was repeated after four weeks. The salivary counts of Streptococcus mutans and Lactobacillus at baseline and 4-week follow-up were determined and presented as the logarithm of colony-forming units per milliliter (log CFU/mL). statistical analysis was performed using independent samples t-test and paired sample t-test (P<0.05). Results: A significant decrease in salivary Streptococcus mutans and Lactobacillus was observed using both toothpastes (*P<0.001). The difference between the antibacterial efficacy of two toothpaste types on Streptococcus mutans and Lactobacillus was not statistically significant (P=0.530, and P=0.137, respectively). Conclusion: Due to the comparable efficacy of the investigated herbal toothpaste with conventional toothpaste, it potentially qualifies as a complementary agent for self-care oral hygiene procedures. Trial registration: This trial was registered in the “Iranian Registry of Clinical Trials” (IRCT20210414050964N1) on 21/06/2021.
Hematological Effects and Benchmark Doses of Long-Term Co-Exposure to Benzene, Toluene, and Xylenes (BTX) in BTX-Exposed Petrochemical Workers Cohort (BEPWC)
Authors: Zhang Zhaorui , Liu Xin , Zhang Xinjie , Zhang Yingying , Deng Na , Lai Guanchao , Yang Aichu , Huang Yongshun , Dang Shanfeng , Zhu Yanqun , Xing Xiumei , Xiao Yongmei , Deng Qifei 
Published: January 2022

Abstract
Background
Ubiquitous benzene, toluene, and xylenes (BTX) frequently occur together. Exposure to single BTX component and BTX-rich mixtures could induce hematological effects. However, it still needs to clarify the hematological influences of long-term co-exposure to BTX components, and propose reference exposure levels (REL) base on their hematological effects.
Objective
We sought to evaluate the hematological effects of long-term BTX co-exposure and estimate REL based on these effects.
Methods
We established BTX-Exposed Petrochemical Workers Cohort (BEPWC), quantified long-term BTX exposure levels by calculating cumulative exposure doses (CED), and detected multiple hematologic parameters in both baseline and follow-up stages. Generalized weighted quantile sum (gWQS) regression models were used to evaluate the combined effects of BTX components and identify their contributions. Benchmark Dose (BMD) Software was used to calculate BMD and the lower confidence limits (BMDL).
Results
Most hematologic parameters were decreased after four-year follow-up (P<0.05). We found a positive association of benzene with the decline in monocyte counts (β = 0.012), and a negative association of toluene with the decline in mean corpuscular hemoglobin concentrations (β =-0.905) after false discovery rate (FDR) adjustment. The associations of BTX components with the decline in hematologic parameters were mostly significantly stronger in subjects with higher baseline parameters, males, drinkers, and overweighted subjects (FDR-adjusted Pinteraction <0.05). BTX components had positive combined effects on the decline in monocyte counts, red blood cell counts, and hemoglobin concentrations (Ptrend for WQS index <0.05). BMD (and BMDL) for CED levels of benzene, toluene, and xylene were estimated at 2.138 (1.559), 1.449 (1.325), and 2.937 (2.312) mg/m3×year, respectively.
Conclusions
Our study revealed complex hematological effects of long-term BTX occupational co-exposure, and proposed some REL-TWA around 0.01 ppm for BTX components based on their hematological effects. All these findings are worthy of further investigation.
Asymmetric Boreal Summer Intraseasonal Oscillation Events Over the Western North Pacific and Their Impacts on East Asian Precipitation
Authors: Li Wen , Yang Xiu-Qun , Fang Jiabei , Tao Lingfeng , Sang Xiaozhuo , Cai Danping , Yin Manman , Sun Xuguang 
Published: January 2022

Abstract
The boreal summer intraseasonal oscillation (BSISO) is the most prominent tropical subseasonal signature especially over the western North Pacific (WNP). Due to restrictions of methodology in extracting BSISO with band-pass filtering or EOF decomposition, most of the previous studies ignored the asymmetry of BSISO. This study reexamines the BSISO events over WNP and their impacts on the East Asian precipitation. With a hierarchical cluster analysis, the BSISO events over WNP during the summers of 1985-2010 are classified into two categories, the long-period (30-60 day) and short-period (10-20 day) events. The long-period BSISO events manifest as a northward propagating mode with a significant phase asymmetry characterized by a fast development, but a slow decay of the intraseasonal convection. The fast development tends to cause a rapid reversal of the atmospheric anomalies over WNP from an anomalous anticyclone induced by the preceding slow convection suppression to an anomalous cyclone, leading to a fast northeastward retreat of the preceding enhanced western North Pacific subtropical high. Accordingly, the middle and lower reaches of Yangtze River valley experience a rapid reversal from the increased precipitation to the decreased, while the precipitation in coastal South China keeps decreased. The short-period BSISO events which are symmetric in phase act as a northwestward propagating mode, mainly affecting East Asian precipitation in an oblique belt extending from southwest China to southern Japan and southern Korean Peninsula. Therefore, the two types of the BSISO events especially the asymmetric long-period BSISO events over WNP and their impacts on the East Asian precipitation revealed in this study would provide a new potential for subseasonal-to-seasonal forecast of the East Asian summer monsoon precipitation.
Inquisition of Electro-Magnetic Riga Plate for Laminar Flow
Authors: Hussain Azad , Akbar Sobia , Arshad Muhammad , Nadeem Sohail 
Published: January 2022

Abstract
The probation is made to study the stagnation point flow of non-Newtonian fluid for Riga plate. Electric potential and magnetic flux density with time dependent flow is examined. Mesh for electric potential, magnetic flux, laminar flow with physics controlled fine, finer and extra finer option is also represented in details. Inquisition is solved in COMSOL Multi-physics 5.4 to obtain the results of surface magnitude, counter, table surface, magnetic flux, electric potential and coarse mesh for velocity, pressure, magnetic and electric fields. Coarse mesh of electric insulation and magnetic flux of the geometry is created with 6067, 18688 domain elements and 901, 1448 boundary elements. Tables for velocity surface, mesh domain, quadrilateral and triangular elements are also presented. Obtained results are discussed with graphs and tables in details.
Heart Sound Classification Based on Fractal Dimension and MFCC Features Using Hidden Markov Model
Authors: Bahreini Mahbubeh , Barati Ramin , Kamaly Abbas 
Published: January 2022

Abstract
Early diagnosis is crucial in the treatment of heart diseases. Researchers have applied a variety of techniques for cardiovascular disease diagnosis, including the detection of heart sounds. It is an efficient and affordable diagnosis technique. Body organs, including the heart, generate several sounds. These sounds are different in different individuals. A number of methodologies have been recently proposed to detect and diagnose normal/abnormal sounds generated by the heart. The present study proposes a technique on the basis of the Mel-frequency cepstral coefficients, fractal dimension, and hidden Markov model. It uses the fractal dimension to identify sounds S1 and S2. Then, the Mel-frequency cepstral coefficients and the first- and second-order difference Mel-frequency cepstral coefficients are employed to extract the features of the signals. The adaptive Hemming window length is a major advantage of the methodology. The S1-S2 interval determines the adaptive length. Heart sounds are divided into normal and abnormal through the improved hidden Markov model and Baum-Welch and Viterbi algorithms. The proposed framework is evaluated using a number of datasets under various scenarios.
Etiology and Chinese Medicine Screening of Severe COVID-19 Based on Multiomics and Serum Pharmacology
Authors: Zhang Yong-Zheng , Yong-Zheng ZHANG , Meng-Jia LI , Yang WU , CHENG Lu-Feng 
Published: January 2022

Abstract
Background

At present, scholars believe that severe COVID-19 is related to a variety of basic diseases, and we also observe this point using multi-omics method. The latest sequencing data of severe COVID-19 patients were combined to analyze the pathological mechanism, and pharmacological experimental research on local drugs was conducted, and a compound ingredient was found to have potential medicinal value.
Results

Here, we observed, for patients with severe COVID-19 disease, the differential miRNA expression is mainly low but having higher expression of mRNA. These differential mRNA expressions are associated with the activation of inflammatory pathways and ultimately with hypoxia and coagulation. Using database analysis, we found that Yi Xin Tong Mai Granule(YXTMG) might regulate COVID-19 through Toll-like receptor signaling pathway by acting on different immune targets. We found a new molecular mechanism for COVID-19 to turn the crisis around, the down-regulated miR-181a-5p mediates the up-regulation of PLAU and SERPINE1 molecules to cause cardiovascular adverse events, and YXTMG may prevent it. At the same time, molecular docking indicated that the its various components have anti-inflammatory activity. In vitro studies, we confirmed that YXTMG had antioxidant and anti-inflammatory activities.
Conclusions

The study has supplemented the potential mechanism for the conversion of mild to critical COVID-19 disease and screened the Chinese medicines for improving these factors, providing methodological reference for disease pathology and drug development.
Serum Cytokine Levels for Predicting Immune-Related Adverse Events and the Clinical Response in Lung Cancer Treated with Immunotherapy
Authors: Zhao Ni , Yi Ye , Cao Wen , Mei Nan , Fu Xiao , Li Chunli 
Published: January 2022

Abstract
Analyses of the composition of peripheral cytokines hold promise for providing a basis for determining the prognosis of lung cancer treated with immunotherapy. In this study, we assessed correlations between interleukins in peripheral blood and the disease prognosis in patients with lung cancer. We retrospectively collected eligible adult patients with histologically confirmed lung cancer. Patients with immune-related adverse events (AE) from immunotherapy had higher pretreatment levels of IL-2 (p=0.002), IL-17 (p=0.01), and IFN-α (p=0.02) than patients with nonimmune-related adverse events (NAE). Univariate analysis showed that changes in IL-2 (p=0.04), IL-5 (p=0.007), IFN-α (p=0.003), IFN-γ (p=0.012) and TNF-α (p=0.049) levels were significantly increased in patients with AE compared with those with NAE before the second cycle of therapy. Patients with a clinical benefit had higher levels of IL-17 before the third cycle than patients without a clinical benefit. No significant cytokine differences were observed between patients with and without a clinical benefit undergoing ICI pretreatment or in the first two cycles of therapy. Plasma cytokines are related to immune-related adverse events and clinical responses, which are potential predictive markers for anti-PD-1/PD-L1 therapy in lung cancer patients and may play an important role in selecting patients who would benefit from PD-1/PD-L1 inhibitors.
Effects of Urbanization Intensity on Glomalin-Related Soil Protein in Nanchang, China: Influencing Factors and Implications for Greenspace Soil Improvement
Authors: Jin Taotao , Liu Wei , Wang Yu , Zhao Ming , Fu Yao , Dong Yulin , Luo Tianyu , Fu Hang , Wang Qiong 
Published: January 2022

Abstract
Glomalin-related soil protein (GRSP) is a stable and persistent glycoprotein secreted by arbuscular mycorrhizal (AM) fungi that plays important roles in sequestering soil organic carbon (SOC) and soil quality improvement. Rapid urbanization has led to serious greenspace soil disturbances, resulting in soil degradation. However, few researches have examined the effects of urbanization on GRSP and its influencing factors. In this study, impervious surface area (ISA) was selected as an indicator of urbanization intensity. A total of 184 soil samples were collected from the 0-20 cm soil layer in the Nanchang greenspace, China (505 km2). The GRSP content, soil properties, urban forest characteristics, and land-use configuration were determined and investigated. The results showed that total GRSP (TG) and easily extractable GRSP (EEG) averages were 2.38 and 0.57 mg·g-1, respectively. TG and EEG decreased by 16.22 % and 19.68 %, respectively, from low to heavy urbanization areas. Linear regression analysis revealed a negative correlation between SOC and GRSP/SOC. SOC decreased from 39.9 to 1.4 mg·g-1, while EEG/SOC and TG/SOC increased by about 17 % and 34 %, respectively, indicating the important contribution of GRSP to the SOC pool. Pearson and redundancy analysis showed that GRSP was positively correlated with soil SOC, P, N, vegetation richness, and tree height but negatively correlated with pH, bulk density, and impervious area. The partial least squares path model (PLS-PM) further showed that urbanization affected soil properties, forest characteristics, and land use factors leading to GRSP changes. This study revealed the effects and key influencing factors of urbanization on GRSP. In the future, urban greenspace soil improvement can be considered from the new perspective of enhancing GRSP soil content.
Chronic Spontaneous Urticaria: A Low Grade Disseminated Intravascular Coagulation Only Partially Reversed by Omalizumab
Authors: Pino Marina Di , Ruberto Maria Filomena , Costanzo Giulia Anna Maria Luigia , Firinu Davide , Piras Maria Sebastiana , Mura Mario Nicola , Giacco Stefano Del , Coghe Ferdinando , Marongiu Francesco , Barcellona Doris 
Published: January 2022

Abstract
Chronic spontaneous urticaria (CSU) is a disorder characterized by wheals and/or angioedema. The coagulation cascade and inflammation pathways are closely linked together. The aim of our study was first to investigate the dynamics of clot formation in plasma (Clot Waveform Analysis, CWA) in a group of 47 patients with CSU along with other coagulative parameters dedicated to the study of hypercoagulability such as: D-Dimer, F 1+2 peptide, Fibrinogen, Platelet count and Mean Platelet Volume (MPV). Secondly, 23 out of 47 patients were treated with Omalizumab at four administration intervals from T0 to T4. A statistically significant increase in Activated Partial Thromboplastin (aPTT) ratio, D-Dimer, F1+2, Platelet count and MPV was found when compared with 53 healthy controls (HC). In contrast, the 2nd Derivative of aPTT showed lower values than those of the HC. No differences were found between 1st derivative of aPTT and Fibrinogen. D-Dimer only showed a significant difference between T0 and T3. An activation of both coagulation and fibrinolysis along with a weaker clot acceleration may be in agreement with a low-grade DIC. The accelerated turnover pf platelets expressed by both an increase of platelet count and MPV further support this pathway in CSU. Omalizumab does not affect the relationship between the immune and the haemostatic systems.
Ecophysiological properties of a cluster root-forming plant, Helicia cochinchinensis (Proteaceae) grown on Miyajima Island, Japan
Authors: Wasaki Jun , Okamura Tadashi , Yamauchi Taiki , Maruyama Hayato , Uchida Shinji , Mukai Seiji , Tsubota Hiromi 
Published: January 2022

Abstract
Aims The family Proteaceae is one of the dominant families in nutrient-impoverished habitats in the Southern hemisphere, and less common in the Northern hemisphere. Helicia cochinchinensis Lour. is the only Proteaceae species in Japan. This study aimed to unveil the ecophysiological properties of H. cochinchinensis grown on Miyajima Island, Hiroshima, Japan.Methods Phosphorus (P) status and dynamics of soils in H. cochinchinensis habitats were measured. Plant P and nitrogen (N) concentrations of leaves were measured after digestion. Roots and rhizosheath soil were collected to assess root morphology and root exudates.Results Available P (Olsen-P) in soils in habitats of H. cochinchinensis was 0.46–3.7 mg P kg-1 soil. Citrate was the major carboxylate in root exudates and its concentration increased during cluster-root formation. Acid phosphatase activity was greater at the surface of cluster roots that on the surface of other roots and bulk soil, especially for mature cluster roots. Sparingly soluble organic P concentrations decreased in the rhizosheath soil of mature cluster roots. The P concentrations of H. cochinchinensis leaves were relatively low; 0.34–0.69 mg P g-1 DW and 0.15–0.29 mg P g-1 DW in mature and senesced leaves, respectively. The P demand of H. cochinchinensis was less than that of nearby trees, showing greater P-remobilization efficiency.Conclusions Phosphorus mobilization from unavailable P by cluster roots supported P uptake by H. cochinchinensis, and P remobilization from senescing leaves contributed to sustain growth under P-deficient conditions.
The Immune Cell Infiltration Patterns and Characterization Score in Bladder Cancer to Identify Prognostic.
Authors: Zhang Yongsheng , Wang Yunlong , Wang Jichuang , Zhang Kaixiang 
Published: January 2022

Abstract
Bladder cancer (BLCA) is among the most frequent types of cancer. Patients with BLCA have a significant recurrence rate and a poor post-surgery survival rate. Recent research has found a link between tumor immune cell infiltration (ICI) and the prognosis of BLCA patients. However, the ICI picture of BLCA remains unclear. Common gene expression data was obtained by combining the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) expression databases. Two computational algorithms were proposed to unravel the ICI landscape of BLCA patients. The R package "limma" was applied to find differentially expressed genes (DEGs). Principal-component analysis (PCA) was used to calculate the ICI score. A total of 569 common gene expression data were retrieved from TCGA and GEO cohorts. CD8+ T cells were found to have a substantial positive connection with activated memory CD4+ T cells and immune score. On the contrary, CD8+ T cells were found to have a substantial negative connection with Macrophages M0. Thirty-eight DEGs were selected. Two ICI patterns were defined by unsupervised clustering method. Patients of BLCA were separated into two groups. The high ICI score group exhibits better outcome than the low one (p < 0.001). Finally, the group with a high tumor mutation burden (TMB) as well as a high ICI score had the best outcome. (p <0.001). Combining TMB and ICI score resulted in a more accurate survival prediction, suggesting that ICI score could be used as a prognostic marker for BLCA patients.