bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel Venetin-1 nanoparticle from earthworm coelomic fluid as a promising agent for the treatment of non-small cell lung cancer
Authors: Rybicka Magda , Czaplewska Paulina , Rzymowska Jolanta , Sofińska-Chmiel Weronika , Wójcik-Mieszawska Sylwia , Lewtak Kinga , Węgrzyn Katarzyna , Jurczak Przemysław , Szpiech Agata , Nowak Jakub , Musiał Natalia , Fiołka Marta J. 
Published: November 2022

AbstractThe present research shows the antitumor activity of a protein-polysaccharide complex Venetin-1 obtained from the coelomic fluid of Dendrobaena veneta earthworms against A549 cancer cells. The investigations are a continuation of experiments on the antitumor activity of coelomic fluid obtained from this species. The Venetin-1 nanoparticle was obtained after thermal treatment of the coelomic fluid, separation from coelomocytes, filtration, and lyophilization. The preparation showed a selective effect on cancer cells, whereas normal cells were unaffected. Venetin-1 was effective against the lung cancer cells at doses of 31.3 and 62.5 µg/ml, and the results were imaged using light microscopy and scanning electron microscopy (SEM). The cells died mainly via the apoptosis pathway. Necrotic cells appeared sporadically in the microscopic view. SEM imaging revealed complete destruction of the A549 cells after the incubation with Venetin-1. The atomic force microscopy (AFM) analyses showed changes in the topography, peak force error images, and Young’s modulus (elasticity) of the A549 cells after the incubation with Venetin-1. The transmission electron cryomicroscopy (Cryo-TEM) analysis indicated a polymeric nature of the analyzed preparation. The samples of Venetin-1 showed a very homogeneous size profile with the microparticle size of approximately 58.23 nm. A significant decrease in Venetin-1 binding to sphingomyelin was observed. Venetin-1 lost its pore-forming activity or deactivation of the pore-forming activity occurred. This confirms the absence of hemolytic capacity of Venetin-1 towards red blood cells. The conducted analyses show the suitability of the obtained complex for biomedical research. The next step will consist in analyses of the effect of Venetin-1 on the immune system in mice.
ROOT parameters of sugarcane and soil compaction indicators under deep strip tillage and conventional tillage
Authors: de Lima Camila Cassante , De Maria Isabella Clerici , da Silva Guimarães Júnnyor Wellingthon , Figueiredo Getulio Coutinho , Dechen Sonia Carmela Falci , Bolonhezi Denizart 
Published: November 2022

AbstractSoil tillage and agricultural traffic generate changes in soil physical attributes and affect the growth of the roots. This study evaluates the impact of system soil tillage on compaction and sugarcane root growth. The experiment was carried out on a Rhodic Kandiudox with two soil tillages (Deep Strip Tillage and Conventional Tillage) and two positions (beds or traffic lane and no traffic lane), totaling four treatments (DST-beds + no traffic lane, DST-traffic lane, CT-no traffic lane and CT-traffic lane). Soil penetration resistance (SPR), bulk density, dry mass, and root system lengths and volumes were evaluated. DST-beds presented lower values for SPR (1.45 MPa) compared to the other treatments (2.55 MPa). This lower SPR did not reflect significant increases in root growth in relation to the DST-traffic lane, meaning that the roots were not confined to the beds. The dry root mass for CT- traffic lane was 35% less than for DST- traffic lane, and CT-no traffic lane reduced of the root dry mass in the layers 0.0–0.2 and 0.2–0.4 m by 62% and 47%, respectively, compared to the DST-beds. Therefore, CT, although widely used, does not create adequate conditions for root development in the first sugarcane cycle, even in lanes with no traffic.
Clinical significance of prostate volume and testosterone reduction on lower urinary tract symptoms in patients with prostate cancer undergoing androgen deprivation therapy
Authors: Bang Woo Jin , Kim Hwanik , Oh Cheol Young , Jo Jung Ki , Cho Jin Seon , Shim Myungsun 
Published: November 2022

AbstractTo investigate the effect of both prostate volume and serum testosterone changes on lower urinary tract symptoms in patients with prostate cancer undergoing androgen deprivation therapy. A total of 167 patients who received androgen deprivation therapy for prostate cancer treatment from January 2010 to August 2020 were enrolled in this retrospective study. Changes in the International Prostate Symptom Score (IPSS) in the patient groups stratified by prostate volume and the amount of testosterone reduction were assessed every 4 weeks until 12 weeks after androgen deprivation therapy initiation. Longitudinal mixed models were used to assess the adjusted effects of prostate volume and testosterone reduction on IPSS change. All mean values of IPSS-total score (IPSS-total), voiding subscore (IPSS-vs), and storage subscore (IPSS-ss) significantly decreased from baseline to week 12 in both patients with small (< 33 mL) and large (≥ 33 mL) prostates. The mean values of IPSS-total, IPSS-vs, and IPSS-ss similarly decreased in patients with large prostate with a baseline IPSS-total of ≥ 13. However, in those with small prostate, IPSS-ss specifically remained unchanged, while IPSS-total and IPSS-vs significantly decreased. In addition, only in patients with small prostate (< 33 mL), patients with lesser testosterone reduction (< Δ400 ng/dL) showed greater improvement in IPSS-ss by 7.5% compared with those with greater testosterone reduction (≥ Δ400 ng/dL). In conclusion, although androgen deprivation therapy generally improves lower urinary tract symptoms, it may worsen specifically storage symptoms in patients with relatively small prostate and greater testosterone reduction. Our finding suggests that testosterone may influence lower urinary tract symptoms in these patients.
Identification of two QTLs, BPH41 and BPH42, and their respective gene candidates for brown planthopper resistance in rice
Authors: Tan Han Qi , Palyam Sreekanth , Gouda Jagadeesha , Kumar Prakash P. , Chellian Santhosh Kumar 
Published: November 2022

AbstractThe brown planthopper (BPH) is the leading cause of insect damage to rice plants and BPH infestations have caused profound losses in rice production since the 1970’s. There is an urgent need to discover new BPH resistance genes to ensure the successful production of rice. Here, a new BPH resistance source provided by SeedWorks International Pvt. Ltd., SWD10, was used for this purpose. QTL mapping using 232 F2 progenies and 216 polymorphic markers revealed two dominant BPH resistance QTLs, BPH41 and BPH42, located on chromosome 4. BPH resistance mechanism test revealed that antibiosis and antixenosis mechanisms both play a role in BPH resistance conferred by these two QTLs. The QTLs were delimited between markers SWRm_01617 and SWRm_01522 for BPH41, and SWRm_01695 and SWRm_00328 for BPH42. Additionally, using RNA-seq data of lines containing the resistant QTLs, we shortlisted four and three gene candidates for BPH41 and BPH42, respectively. Differential gene expression analysis of lines containing the QTLs suggested that SWD10 BPH resistance is contributed by the plant’s innate immunity and the candidate genes may be part of the rice innate immunity pathway. Currently, the newly identified QTLs are being utilized for breeding BPH resistant rice varieties and hybrids.
Detection of mitochondrial DNA mutations in circulating mitochondria-originated extracellular vesicles for potential diagnostic applications in pancreatic adenocarcinoma
Authors: Vikramdeo Kunwar Somesh , Anand Shashi , Khan Mohammad Aslam , Khushman Moh’d , Heslin Martin J. , Singh Seema , Singh Ajay Pratap , Dasgupta Santanu 
Published: November 2022

AbstractThere is a complete lack of highly sensitive and specific biomarkers for early pancreatic ductal adenocarcinoma (PDAC) diagnosis, limiting multi-modal therapeutic options. Mitochondrial DNA (mtDNA) is an excellent resource for biomarker discovery because of its high copy number and increased mutational frequency in cancer cells. We examined if mtDNA mutations can be detected in circulating extracellular vesicles (EVs) of PDAC patients and used for discerning between cancer and non-cancer subjects. A greater yield of circulating EVs (~ 1.4 fold;p = 0.002) was obtained in PDAC patients (n = 20) than non-cancer (NC) individuals (n = 10). PDAC-EVs contained a higher quantity of total DNA (~ 5.5 folds;p = 0.0001) than NC-EVs and had greater enrichment of mtDNA (~ 14.02-fold;p = 0.0001). PDAC-EVs also had higher levels of cardiolipin (a mitochondrial inner-membrane phospholipid), suggestive of their mitochondrial origin. All mtDNA mutations in PDAC-EVs were unique and frequency was remarkably higher. Most mtDNA mutations (41.5%) in PDAC-EVs were in the respiratory complex-I (RCI) (ND1-ND6), followed by the RCIII gene (CYTB; 11.2%). Among the non-coding genes, D-Loop andRNR2exhibited the most mutations (15.2% each). Altogether, our study establishes, for the first time, that mtDNA mutations can be detected in circulating EVs and potentially serve as a tool for reliable PDAC diagnosis.
Optimism bias and its relation to scenario valence, gender, sociality, and insecure attachment
Authors: Dricu Mihai , Moser Dominik A. , Aue Tatjana 
Published: November 2022

AbstractOptimism bias refers to the tendency to display unjustified high/low expectations of future positive/negative events. This study asked 202 participants to estimate the likelihood of 96 different events. We investigated optimism biases for both oneself and the general population, and how these biases are influenced by gender, valence of the event, sociality of the event, as well as attachment anxiety and attachment avoidance. We found that sociality interacted with gender, with the difference in optimism bias for social vs. alone events being larger among women than among men. Attachment anxiety mainly reduced the optimism bias among men deliberating over future alone situations, while attachment avoidance primarily reduced optimism bias among female respondents deliberating over future social interactions. These results may have implications for the well-being and motivation of differently attached men and women and ultimately inspire psychotherapy interventions.
Fused Raman spectroscopic analysis of blood and saliva delivers high accuracy for head and neck cancer diagnostics
Authors: Koster Hanna J. , Guillen-Perez Antonio , Gomez-Diaz Juan Sebastian , Navas-Moreno Maria , Birkeland Andrew C. , Carney Randy P. 
Published: November 2022

AbstractAs a rapid, label-free, non-destructive analytical measurement requiring little to no sample preparation, Raman spectroscopy shows great promise for liquid biopsy cancer detection and diagnosis. We carried out Raman analysis and mass spectrometry of plasma and saliva from more than 50 subjects in a cohort of head and neck cancer patients and benign controls (e.g., patients with benign oral masses). Unsupervised data models were built to assess diagnostic performance. Raman spectra collected from either biofluid provided moderate performance to discriminate cancer samples. However, by fusing together the Raman spectra of plasma and saliva for each patient, subsequent analytical models delivered an impressive sensitivity, specificity, and accuracy of 96.3%, 85.7%, and 91.7%, respectively. We further confirmed that the metabolites driving the differences in Raman spectra for our models are among the same ones that drive mass spectrometry models, unifying the two techniques and validating the underlying ability of Raman to assess metabolite composition. This study bolsters the relevance of Raman to provide additive value by probing the unique chemical compositions across biofluid sources. Ultimately, we show that a simple data augmentation routine of fusing plasma and saliva spectra provided significantly higher clinical value than either biofluid alone, pushing forward the potential of clinical translation of Raman spectroscopy for liquid biopsy cancer diagnostics.
Exploration of a novel and efficient source for production of bacterial nanocellulose, bioprocess optimization and characterization
Authors: El-Naggar Noura El-Ahmady , El-Malkey Sahar E. , Abu-Saied M. A. , Mohammed A. B. Abeer 
Published: November 2022

AbstractThe demand for bacterial nanocellulose is expected to rise in the coming years due to its wide usability in many applications. Hence, there is a continuing need to screen soil samples from various sources to isolate a strain with a high capacity for bacterial nanocellulose production. Bacillus sp. strain SEE-12, which was isolated from a soil sample collected from Barhiem, Menoufia governorate, Egypt, displayed high BNC production under submerged fermentation. Bacillus sp. strain SEE-12 was identified as Bacillus tequilensis strain SEE-12. In static cultures, BNC was obtained as a layer grown in the air liquid interface of the fermentation medium. The response surface methodology was used to optimise the process parameters. The highest BNC production (22.8 g/L) was obtained using 5 g/L peptone, 5 g/L yeast extract, 50%, v/v Cantaloupe juice, 5 g/L Na2HPO4, 1.5 g/L citric acid, pH 5, medium volume of 100 mL/250 mL conical flask, inoculum size 5%, v/v, temperature 37 °C and incubation time 6 days. The BNC was purified and characterized by scanning electron microscopy (SEM), Energy-dispersive X-ray (EDX) spectroscopy, differential scanning calorimetry (DSC), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and transmission electron microscopy (TEM).
Association between thyroid cancer and cardiovascular disease risk: a nationwide observation study
Authors: Tsai Ming-Chieh , Hsieh Cheng-Tzu , Hsu Hsin-Yin , Yeh Tzu-Lin , Lee Wen-Chung , Chiang Chun-Ju , Hsiao Bo-Yu , Jhuang Jing-Rong , Tsai Wen-Hsuan , Cheng Shih-Ping , Chou Chao-Liang , Lee Chun-Chuan , Liu Sung-Chen , Tseng Po-Jung , Chien Kuo-Liong 
Published: November 2022

AbstractTreatment with levothyroxine and radioiodine contribute alternative cardiovascular function in adults with thyroid cancer. The risks of long-term cardiovascular conditions among thyroid cancer patients is unknown. This study aimed to compare the incidence of coronary heart disease (CHD), ischemic stroke (IS), and atrial fibrillation (AF) among adults with thyroid cancer with that of the general population, especially when stratified by age (< 65 and ≥ 65 years old). This observational cohort study enrolled patients between January 1, 2011 and December 31, 2016 with a follow-up until December 31, 2018. This study analyzed the data of Taiwanese thyroid cancer patients registered on the National Taiwan Cancer Registry Database, with CHD and IS. SIR models were used to evaluate the association between thyroid cancer and CHD, IS, AF, and cardiovascular disease outcome, stratified by age and sex. SIR analyses were also conducted for both sexes, age groups (< 65, ≥ 65 years), and different follow-up years. After excluding 128 individuals (< 20 years or ≥ 85 years old) and with missing index data, 4274 eligible thyroid cancer patients without CHD history, 4343 patients without IS history, and 4247 patients without AF history were included for analysis. During the median follow-up of 3.5 (1.2) years among thyroid cancer patients, the observed number of new CHD events was 70; IS, 30; and AF, 20, respectively. The SIR was significantly higher for CHD (SIR, 1.57; 95% confidence interval [CI] 1.2–1.93) among thyroid cancer patients compared with the age- and sex-specific standardized population. However, the association between thyroid cancer and the risks of IS (SIR, 0.74; 95% CI 0.47–1), cardiovascular disease (SIR, 0.88; 95% CI 0.7–1.05), and atrial fibrillation (SIR, 0.74; 95% CI 0.42–1.06) were insignificant. Moreover, stratification by age < 65 or age ≥ 65 years old and by sex for CHD suggested that the diagnosis of thyroid cancer in the young may attenuate the CHD risk (SIR, 2.08; 95% CI 1.5–2.66), and the CVD risk was constant among both men (SIR, 1.63; 95% CI 1.03–2.24) and women (SIR, 1.53; 95% CI 1.06–1.99). The patients had persistent higher CHD risk for 5 years after cancer diagnosis. Thyroid cancer survivors have a substantial CHD risk, even at long-term follow-up, especially in those patients < 65 years old. Further research on the association between thyroid cancer and CHD risk is warranted.
Fish feeds supplemented with calcium-based buffering minerals decrease stomach acidity, increase the blood alkaline tide and cost more to digest
Authors: Goodrich Harriet R. , Berry Alex A. , Montgomery Daniel W. , Davison William G. , Wilson Rod W. 
Published: November 2022

AbstractPredatory fish in the wild consume whole prey including hard skeletal parts like shell and bone. Shell and bone are made up of the buffering minerals calcium carbonate (CaCO3) and calcium phosphate (Ca3(PO4)2). These minerals resist changes in pH, meaning they could have physiological consequences for gastric acidity, digestion and metabolism in fish. Using isocaloric diets supplemented with either CaCO3, Ca3(PO4)2 or CaCl2 as non-buffering control, we investigated the impacts of dietary buffering on the energetic cost of digestion (i.e. specific dynamic action or SDA), gastric pH, the postprandial blood alkalosis (the “alkaline tide”) and growth in juvenile rainbow trout (Oncorhynchus mykiss). Increases in dietary buffering were significantly associated with increased stomach chyme pH, postprandial blood HCO3−, net base excretion, the total SDA and peak SDA but did not influence growth efficiency in a 21 day trial. This result shows that aspects of a meal that have no nutritional value can influence the physiological and energetic costs associated with digestion in fish, but that a reduction in the SDA will not always lead to improvements in growth efficiency. We discuss the broader implications of these findings for the gastrointestinal physiology of fishes, trade-offs in prey choice in the wild, anthropogenic warming and feed formulation in aquaculture.
Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems
Authors: Kajiura Hiroyuki , Tatematsu Ken-ichiro , Nomura Tsuyoshi , Miyazawa Mitsuhiro , Usami Akihiro , Tamura Toshiki , Sezutsu Hideki , Fujiyama Kazuhito 
Published: November 2022

AbstractThe silkworm,Bombyx mori, is an attractive host for recombinant protein production due to its high expression efficiency, quality, and quantity. Two expression systems have been widely used for recombinant protein production inB. mori: baculovirus/silkworm expression system and transgenic silkworm expression system. Both expression systems enable high protein production, but the qualities of the resulting recombinant proteins have not been well evaluated. In this study, we expressed bovine interferon γ (IFN-γ) using the two systems and examined the quality of the resulting proteins in terms ofN-glycosylation and protein cleavage. Both expression systems successfully produced IFN-γ as anN-glycoprotein. Although the production in the baculovirus/silkworm expression system was much more efficient than that in the transgenic silkworm expression system, unexpected variants of IFN-γ were also produced in the former system due to the differentN-glycosylation and C-terminal truncations. These results indicate that while high protein production could be achieved in the baculovirus/silkworm expression system, unintentional protein modification might occur, and therefore protein expression in the transgenic silkworm expression system is preferable from the point-of-view ofN-glycosylation of the recombinant protein and evasion of unexpected attack by a protease inB. mori.
Weber number and the outcome of binary collisions between quantum droplets
Authors: Alba-Arroyo J. E. , Caballero-Benitez S. F. , Jáuregui R. 
Published: November 2022

AbstractA theoretical analysis of binary collisions of quantum droplets under feasible experimental conditions is reported. Droplets formed from degenerate dilute Bose gases made up from binary mixtures of ultracold atoms are considered. Reliable expressions for the surface tension of the droplets are introduced based on a study of low energy excitations of their ground state within the random phase approximation. Their relevance is evaluated considering an estimation of the expected excitation energy having in mind the Thouless variational theorem. The surface tension expressions allow calculating the Weber number of the droplets involved in the collisions. Several regimes on the outcomes of the binary frontal collisions that range from the coalescence of the quantum droplets to their disintegration into smaller droplets are identified. Atoms losses of the droplets derived from self-evaporation and three-body scattering are quantified for both homo- and hetero-nuclear mixtures. Their control is mandatory for the observation of some interesting effects arising from droplets collisions.
Strategies of protected area use by Asian elephants in relation to motivational state and social affiliations
Authors: Madsen Anastasia E. , Minge Christin , Pushpakumara T. V. , Weerathunga U. Sameera , Padmalal U. K. , Weerakoon Devaka K. , de Silva Shermin 
Published: November 2022

AbstractAnimals’ space requirements may vary according to life-history and social considerations. We observed 516 wild adult Asian elephants from both sexes, over 9 years, to investigate how life-history traits and social behavior influence protected-area (PA) use at Udawalawe National Park, Sri Lanka. Male PA-use, quantified in terms of average between-sightings-interval (BSI), was significantly influenced by the interaction of age class and motivational state (i.e. reproduction vs. foraging). Musth lengthened with age, with a median of 24.5 days for ages 21–30, 32.5 days for ages 31–40, and 45 days for those > 40. A minority (11%) used it exclusively during musth, while others used it exclusively for foraging (44%) or both (45%). Males using it in both states and older musth-only males were more likely to be seen across years. There were 16 social communities containing between 2–22 adult females. Females’ BSI was significantly influenced by social ties, but this relationship was weak, because members of social communities do not necessarily disperse together, resulting in high individual variation in space-use. Inter-annual variability in sightings among individuals of both sexes indicates that around ¾ of the population is likely non-residential across years, challenging the prevailing fortress-conservation paradigm of wildlife management.
Data-driven comorbidity analysis of 100 common disorders reveals patient subgroups with differing mortality risks and laboratory correlates
Authors: Koskinen Miika , Salmi Jani K. , Loukola Anu , Mäkelä Mika J. , Sinisalo Juha , Carpén Olli , Renkonen Risto 
Published: November 2022

AbstractThe populational heterogeneity of a disease, in part due to comorbidity, poses several complexities. Individual comorbidity profiles, on the other hand, contain useful information to refine phenotyping, prognostication, and risk assessment, and they provide clues to underlying biology. Nevertheless, the spectrum and the implications of the diagnosis profiles remain largely uncharted. Here we mapped comorbidity patterns in 100 common diseases using 4-year retrospective data from 526,779 patients and developed an online tool to visualize the results. Our analysis exposed disease-specific patient subgroups with distinctive diagnosis patterns, survival functions, and laboratory correlates. Computational modeling and real-world data shed light on the structure, variation, and relevance of populational comorbidity patterns, paving the way for improved diagnostics, risk assessment, and individualization of care. Variation in outcomes and biological correlates of a disease emphasizes the importance of evaluating the generalizability of current treatment strategies, as well as considering the limitations that selective inclusion criteria pose on clinical trials.