bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mouse embryonic stem cells – a new cellular system for studying the equine infectious anemia virus <i>in vitro</i> and <i>in vivo</i>
Authors: Savchenkova I. P. , Alekseyenkova S. V. , Yurov K. P. 
Published: May 2022

The complexity of the pathogenesis and insufficient knowledge about the slow retroviral infections, which include equine infectious anemia, necessitates finding an adequate laboratory model for the study of the infection process and immunogenesis to create means of prevention and treatment of diseases. Data about strains and cellular tropism of the virus are discussed. It was shown that mouse embryonic stem cells (ESCS) exhibited unique properties and characteristics. In contrast to fibroblasts and other cell types, these cells can be considered as a new cell system for studying EIAV in vitro and in vivo. Under differentiation-inducing conditions they are able to reproduce in vitro embryogenesis cells and form cells of three germ layers. Differentiation of mouse ESCs in the direction of hematopoiesis could contribute new knowledge and understanding of viral tropism EIAV in vitro. ESC can be returned back to the early pre-implantation embryo. Once in the germ cell environment, they participate in the formation of tissues and organs of the developing fetus. Thus, the adaptation of the mouse ESC to the equine EIAV through genetic transformation makes it possible to get closer to the creation of a laboratory model for the study of the in vivo immune response in the lentiviral infection.
Interaction of herpesviruses with mature human spermatozoa in the model system <i>in vitro</i>
Authors: Malolina E. A. , Lebedeva A. L. , Kulibin A. Yu. , Evdokimov V. V. , Kurilo L. F. , Sorokina T. M. , Tulenev Ju. A. , Naumenko V. A. , Kushch A. A. 
Published: May 2022

The DNA of human herpesviruses (HHV), including the herpes simplex virus (HSV) and cytomegalovirus (CMV), is often identified in ejaculates of patients with urogenital diseases and infertility. At least a part of viral DNA is associated with cell fraction of ejaculate. However, it remains unclear how the semen is infected by the virus. It can be located in gametes or be capable of infecting mature germ cells, including motile sperm cells. In order to resolve this issue, interactions of the CMV and HSV with human sperm cells were studied using an original optimized model of the herpesviral infection of male gametes in vitro. The analysis of the immunofluorescent staining of gametes for viral antigens has shown that CMV infected 2% gametes, while HSV infected 17.26 ± 2.58% gametes. The fraction of progressively motile sperm cells contained 13.99 ± 4.64% infected cells. Localization of HSV was studied by the confocal microscopy. Sometimes, viral gB protein was found on sperm cell membrane. In addition, optical scanning of other cells has shown the intracellular localization of the viral proteins. In the majority of spermatozoa, the viral proteins were observed in the head and neck. In some cells, they were located in the middle piece or, rarely, in the equatorial segment. In general, after in vitro infection HSV antigens were located in the same areas of the sperm cells as in ejaculates from infected patients. According to DNA–DNA hybridization in situ, gametes containing HSV DNA accounted for 16.94 ± 5.28%, which is consistent with the results obtained in the immunofluorescence assay. It can be concluded that mature male gametes are infected by HHV in the genital tract, where the virus binds to the sperm cell membrane and enters the cell. Interaction of HHV with progressively motile sperm cells implies a vertical viral transmission upon fertilization and points to the necessity of testing ejaculate for herpesviruses infections.
Investigation on Acoustic Emission Characteristics of Hole-Joint Contained Granite under a Compressive Disturbance: Experimental Insights
Authors: Li Peng , Cai Meifeng , Guo Qifeng , Ren Fenhua 
Published: May 2022

Abstract
Uniaxial compression experiments were performed in cross-jointed granite samples with a hole, together with acoustic emission (AE) observations, to investigate AE variability and precursory information during sample failure. The variability characteristics of multiple AE time-series parameters (including AE counts, accumulated counts, energy, accumulated energy, and b-value) and frequency domain parameters (including AE frequency and amplitude) were systematically analyzed, the variations in different parameters and precursor characteristics were compared, and the AE parameters suitable for rock failure prediction in a cavern were discussed. The results indicate that the variability characteristics of different AE parameters are different, and each parameter has a precursor phenomenon of specimen fracture. The peak value of specimen AE counts does not necessarily appear at the peak stress, but the AE counts decrease significantly when the samples are damaged. The accumulated AE count curve makes it easier to identify the precursors of sample failure. The AE energy is released at an accelerated speed when the samples are near complete failure, and a critical energy release exists in the samples. The abrupt increase in AE energy has potential as a precursor to sample failure. The b-value shows a fluctuating decline during the whole loading process and a rapid decline before sample failure. The low-frequency with high-amplitude and the high-frequency with low-amplitude signals exhibit distinct characteristics at different specimen deformation stages and are sensitive to the change in specimen stress state, so they can be used as the crucial signal source for predicting specimen failure. The joint analysis of these AE parameters can better capture the precursory information of rock failure.
Analysis of influencing factors of diabetic foot minor amputation
Authors: wang fenglin , Zhu Di , Zhou LuYao , Liang JieMei , Yang CaiZhe 
Published: May 2022

Abstract
Objective: To investigate the factors that influence diabetic foot (DF) minor amputation. Methods: In this case-control study, the clinical data of 955 hospitalized patients with DF were retrospectively analyzed, according to whether hospitalization amputation was divided into minor amputation and the non-amputation group, compared two groups of general data, laboratory examination, diabetes complications and complications, such as differences, multiple factors regression analysis DF Risk factors associated with minor amputation in patients. Results: There were statistically significant differences between the two groups in DPN, DR, PAD, ABI, TBI, and Wagner grades, as well as age, sex, HbA1c, FPG, Scr, SUA, TC, ALB, HDL-C, WBC, and Hb (P<0.05). The logistic regression analysis that HbA1c (odds ratio [OR] 1.082 [95% CI 1.011–1.158], p= 0.023), ABI＜0.9 (odds ratio [OR] 1.793 [95% CI 1.316–2.443],p=0.000), TBI＜0.7(odds ratio [OR] 2.569 [95% CI 1.889–3.495], p=0.000), Wagner classification (odds ratio [OR] 2.792 [95% CI 2.303–3.384], p=0.000) and PAD (odds ratio [OR] 2.343 [95% CI 1.731–3.170], p=0.000) were significant risk factors for DF minor amputation (P＜0.05). Higher Hb (odds ratio [OR] 0.981 [95% CI 0.973–0.988], p=0.000) was an independent protective factor for minor amputation. Conclusion:HbA1c, lower ankle brachial index level, and lower toe-brachial index level were all related with minor amputation. Wagner classification and diabetic peripheral angiopathy may represent a novel independent factor. In light of these concerns, early preventive and timely multidisciplinary assistance is critical to prevent diabetic foot minor amputation.
Deficiency of murine UFM1-specific E3 ligase causes microcephaly and inflammation
Authors: Zhang Jie , Zhu Huabin , Liu Siyang , Quintero Michaela , Zhu Tianyi , Xu Renshi , Cai Yafei , Han Ye , Li Honglin 
Published: May 2022

Abstract
The UFM1 conjugation system is a Ubiquitin (Ub) -like modification system that is essential for animal development and normal physiology of multiple tissues and organs. It consists of UFM1, a Ub-like modifier, and the UFM1-specific enzymes (namely E1 enzyme UBA5, E2 enzyme UFC1 E2, and E3 ligases) that catalyze conjugation of UFM1 to its specific protein targets. Clinical studies have identified rare genetic variants in human UFM1, UBA5 and UFC1 genes that were linked to early-onset encephalopathy and defective brain development, strongly suggesting the critical role of the UFM1 system in the nervous system. Yet the phsyiologica function of this system in adult brain remains not defined. In this study, we investigated the role of UFM1 E3 ligase in adult mouse and found that both UFL1 and UFBP1 proteins, two components of UFM1 E3 ligase, are essential for survival of mature neurons in adult mouse. Neuron-specific deletion of either UFL1 or UFBP1 led to significant neuronal loss and elevation of inflammatory response. Interestingly, loss of one allele of UFBP1 genes caused occurance of seizure-like events. Our study has provided genetic evidence for the indispensable role of UFM1 E3 ligase in mature neurons and further demonstrated the importance of the UFM1 system in the nervous system.
Hemabate Increases Cell Adhesion by Inducing Cx43 Protein Up-expression On HUVECs
Authors: Zhang Yanling , Deng Zhizhao , Li Xianlong , Zhang Qian , Zeng Weiqi , Cai Jun , Yuan Dongdong 
Published: May 2022

Abstract
Background
Hemabate is one of the most frequently-used drug in obstetrics, mostly applied in postpartum hemorrhage. Its hemostatic effect is explicit via inducing intensive vasoconstriction. Inevitably, it may result in vascular endothelial injury. Connexin 43 (Cx43) is reported to be of great importance in vascular damage, and triggers monocyte-endothelial adhesion, which changes hemodynamics and worsens vascular injury. Therefore, we conducted researches on the hemabate-induced monocyte-endothelial adhesion and preliminarily explored the underlying mechanism.
Results
We found that the expression level of Cx43 was much higher on HUVECs cultured with hemabate, which could activate downstream PKCα/MEK/ERK signaling pathway, manifested as PKCα, MEK and ERK phosphorylation levels increase. Then, cell adhesion molecules, such as ICAM-1, VCAM-1 and E-selectin, and THP-1/HUVECs adhesion were all elevated. With the inhibition of Cx43 expression with oleamide, the activity of PKCα/MEK/ERK signaling pathway was downregulated and cell adhesion molecules were attenuated. Also, the effect of hemabate on THP-1/HUVECs adhesion were reversed.
Conclusions
Hemabate application could induce cell adhesion molecules expression and increase monocyte-endothelial adhesion, the underlying mechanism of which was that hemabate upregulated Cx43 expression and activated PKCα/MEK/ERK signaling pathway. This side effect of hemabate should be noticed by clinicians.
Assessment of clinical and subclinical endometritis impacts on the reproductive performance and milk production of dairy cows in Brazilian herds
Authors: Paiano Renan Braga , Bonilla Jeannine , Pugliesi Guilherme , Moreno Andrea Micke , Baruselli Pietro Sampaio 
Published: May 2022

Abstract
Clinical endometritis (CE) and subclinical endometritis (SE) are one of the main uterine diseases in dairy cows during the puerperium period, which can directly affect milk production and fertility in dairy herds. The aim of this study was to investigate the effects of the CE and SE on the subsequent reproductive performance and milk production of dairy cows in Brazilian herds. A total of 279 lactating Holstein dairy cows (28 to 33 d in milk) from six commercial farms were evaluated. The cows were classified into three groups: healthy cows (without CE and SE, n = 161), cows with CE (vaginal discharge score = 3 and ≥ 18% PMNL, n = 83) and cows with SE (absence of signs of CE and > 18% PMNL, n = 35). Cows with CE and SE had a lower (P < 0.05) conception rate at first AI than healthy cows. Furthermore, both cows with CE and SE needed more service to become pregnant and days to be pregnant (P < 0.05) than healthy cows. Cows with CE and SE produced less milk than healthy cows. The logistic regression showed that any factor evaluated in this study were not associated with the incidence of CE and SE. In conclusion, our results reveal a negative impact of CE and SE on the reproductive performance and milk production of dairy cows.
Stochastic model in neural network - an application in pacemaker GABAergic neurons
Authors: Guimarães Karine , Duarte Aline 
Published: May 2022

Abstract
In this paper we present a numerical study of a mathematical model that describes the continuous temporal evolution of membrane potentials in a GABAergic neuronal network. This model, with stochastic and evolutionary characteristics, is an application of the version introduced in1 where the authors present the continuous time version of a new class of stochastic models for biological neural networks.More specifically, the computational simulations of the model provide numerical results describing spiking neuron with electrophysiological characteristics of a brain region called substantia nigra pars reticulada, emphasizing changes in desynchronized firing activity and how changes in individual activity propagate through the network.
HSP expression depends on its molecular construction and different organs of the chicken: a meta-analysis
Authors: Siddiqui Sharif Hasan , Khan Mousumee , Choe Hosung , Kang Darae , Shim Kwanseob 
Published: May 2022

Abstract
Heat shock proteins (HSPs) expression protect the cell from stress, this expression varies on tissue and stress level. Here, we investigated the structure and functional expression of HSPs in different chicken organs using meta-analysis. A total of 1,253 studies were collected from three different electronic databases from January 1, 2015 to February 1, 2022. Of these studies, 28 were selected based on the specific criteria for this meta-analysis. The results for the expression of HSPs and the comparative expression of HSPs (HSP90, HSP70, and HSP60) in different chicken organs (brain, heart, liver, muscle, and intestine) were analyzed using the odds ratio or the random-effects model (REM) at a confidence interval (CI) of 95%. Compared to the thermoneutral groups, heat stress groups exhibited a significant (P < 0.01) change in their HSP70 expression in the chicken liver (8 trials: REM = 1.41, 95% CI: 0.41, 4.82). The expression of different HSPs in various chicken organs varied and the different organs were categorized according to their expression levels. HSP expression differed among the heart, liver, and muscle of chickens. HSPs expression level depends on the structure and molecular weight of the HSPs, as well as the type of tissue.
Clinical manifestations and genotype of primary ciliary dyskinesia diagnosed in Korea: a nationwide, multicenter, retrospective study
Authors: Kim Minji , Lee Mi-Hee , Hong Soo-Jong , Yu Jinho , Cho Joongbum , Suh Dong In , Kim Hyung Young , Kim Hye-young , Jung Sungsu , Lee Eun , Lee Sooyoung , Jeong Kyunguk , Shim Jung Yeon , Kim Jeong Hee , Chung Hai Lee , Jang Yoon Young , Kwon Ji-Won , Seo Ju-Hee , Kim Ju Hee , Ahn Ji Young , Song Kun Baek , Song Kyu-Sang , Kim So Yeon , Kim Seon Young , Chung Eun Hee 
Published: May 2022

Abstract
Background: Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder that leads to secondary ciliary dysfunction. PCD is a rare disease worldwide, and its data are limited in Korea. This study systematically evaluated the clinical symptoms, diagnostic characteristics, and treatment of pediatric PCD in Korea. Methods: The medical records of paediatric patients diagnosed with PCD from January 2000 to August 2021 were retrospectively evaluated in this Korean nationwide, multicenter study.Results: Overall, 42 patients were diagnosed with PCD in 15 medical institutions. The median age at diagnosis was 9.9±6.0 years (range: 0.5 months–25.5 years). Most patients (40/42) were born at full term, 18 (42.9%) had neonatal respiratory symptoms, and 15 (35.7%) had a history of admission to the neonatal intensive care unit. The most common complaint (59.5%) was chronic nasal symptoms. Among them, 18 patients had a PICADAR score over 5 points, 32 patients were diagnosed through transmission electron microscopy (TEM) and 14 patients through genetic studies. TEM mostly identified outer dynein arm defects (alone or combined with inner dynein arm defects, n = 16). The genes with the highest mutation rates were DNAH5 (three cases) and DNAAF1 (three cases). Chest computed tomography revealed bronchiectasis in 34 out of 42 patients.Conclusions: Although PCD is a rare disease, early diagnosis can improve prognosis. However, a single gold standard diagnostic method has not yet been established, and its prevalence may be low owing to misdiagnosis.
Percutaneous coronary angiography and interventions in the porcine model: A feasible and safe proposal
Authors: Tsigkas Grigorios , Vasilagkos Georgios , Tousis Alexandros , Theofanis Michail , Apostolos Anastasios , Spyridonidis Ioannis , Goudas Leonidas , Karpetas Georgios , Moulias Athanasios , Katsouras Christos S. , Kitrou Panagiotis , Mplani Virginia , Moulas Anargyros N , Karnabatidis Dimitrios , Davlouros Periklis 
Published: May 2022

Abstract
Background/Purpose
Coronary angiography and percutaneous coronary intervention (PCI) procedural details in swine are similar to those performed to humans, since their heart and coronary anatomy closely resembles. However, only a few detailed descriptions of the procedure are available, containing notable differences. We present a feasible and reproducible protocol for percutaneous coronary interventions in porcine experimental models, utilizing ultrasound-guided femoral approach.
Methods/Materials
Nine female pigs were studied to explore the feasibility of superficial femoral arterial (SFA) access for coronary angiography and provisional PCI, as well as the most suitable guiding coronary catheters and angiographic projections for the above interventions. Experiments were performed under general anesthesia, using ultrasound-guided puncture of the SFA to gain arterial access. The Amplatzer AR1® catheter, and the Right Coronary Bypass® catheter were used for the selective engagement of the right and the left coronary artery respectively.
Results
Successful arterial access and subsequent cardiac catheterization were performed in all pigs. Only one animal required a second puncture for femoral artery access. None of the 9 animals presented any significant tachycardia or hypotensive episode. One animal developed an access site-related complication following the first catheterization procedure. During follow-up, 100% success of SFA catheterization was achieved using the same ultrasound-guided technique.
Conclusions
The ultrasound-guided superficial femoral artery access for coronary angiography and provisional interventions in porcine models is a quick and safe alternative to the carotid artery approach. The RCB and AR1 catheters may be the best choice for the quick and easy selective coronary engagement of the right and left ostia respectively.
WITHDRAWN: Data Mining and Analysis for Adverse Event Signals of Emicizumab Based on Food and Drug Administration Adverse Event Reporting System Database
Authors: Wei Lianhui , Tian Ye , Chen Xiao , Guo Xiaojing , Chen Chenxin , Zheng Yi , Xu Jinfang , Ye Xiaofei 
Published: May 2022

Abstract
Background: Emicizumab is the latest treatment for patients with hemophilia A. Its safety in real-world data is limited, and both regulatory agencies and clinical researchers have raised concerns about its risk of adverse events.Aim: This study utilized the Food and Drug Administration Adverse Event Reporting System database to tap into potential adverse event signals of Emicizumab to inform its clinical use and further protect patient health.Method: The analysis was carried out by searching data from the fourth quarter of 2017 to the second quarter of 2021 in the Food and Drug Administration Adverse Event Reporting System database. Adverse events were defined as the preferred term in the International Council for Harmonisation Medical Dictionary for Regulatory Activities version 24.0. Disproportionality analysis was performed using the reporting odds ratio and information component methods based on statistical shrinkage transformation.Results: A total of 5,598,717 records were included, of which 1,244 were emicizumab-related adverse event records. A total of 703 emicizumab-related adverse event signals were mined, and 101 positive signals were detected. Adverse events such as haemarthrosis, haemorrhage, haematoma muscle, and traumatic haemorrhage had stronger signal intensity, while haemorrhage, haemarthrosis, arthralgia, fall, and injection site pain were reported more frequently.Conclusion: Emicizumab has an adequate safety profile, with adverse drug reactions mostly consisting of arthralgia and injection site reactions with mild symptoms. Attention should be paid to potential adverse drug reactions such as acute myocardial infarction and sepsis, and certain precautions should be taken to ensure patient safety.
Indirect coagulation of residual lesions of circumferential piles after excisional hemorrhoidectomy; a prospective controlled pilot study
Authors: Hamed Mohamed KF , Sabry Ahmed Mohamed , Elazazy Mohamed 
Published: May 2022

Abstract
Background
“Circumferential prolapsed piles” poses great challenge for colorectal surgeons. “Infrared photocoagulation” (IFC) is a safe procedure for early stages of piles but is not readily available due to high costs. We introduce a simple simulation of (IFC) for management of residual daughter piles after surgical excision of the main piles.
Method
10 patients with circumferential grade III-IV piles were recruited for “indirect cauterization” of residual daughter piles after open excision of the main piles and were observed for one month for postoperative complications. The results were compared to those of the last 10 correlated patients in the database managed by simple excision of the main piles only.
Results
Three cases of SSI (Surgical Site Infection) with minimal, temporary spotting after removal of dressing were found in the study group. No case of incontinence was recorded
Conclusion
The “indirect cauterization” technique is feasible with satisfactory outcome for cases of circumferential piles in low resource areas.
Customer churn prediction from Internet banking transactions data using an ensemble meta-classifier algorithm
Authors: Ehsani Fatemeh 
Published: May 2022

Abstract
With the advancement of electronic markets platforms, customers show different purchase behaviors. Since they have a wide range of choices and low exit barriers, customer movement from one digital store to another has become a natural problem for every business. Customers are the most valuable property of every enterprise. Thus, Suppliers have to tie a strong relationship with their customers to prevent their leaving by providing the most appropriate produces and services based on their desires and trying to satisfy them. Customer churn prediction (CCP) is one of the customer relationship management (CRM) strategies to estimate the probability of their abandonment. Marketers use CCP to attract visitors, engage them in websites activities, convert them into customers, and retain them for a long time. Internet banking transactions dataset is a dependable resource to analyze customer interactions and their churn behaviors. Transactions data scrutinizes the customer’s specifications and their payment details. In this paper, we introduced a meta-classifier algorithm to predict customer churn behavior according to the transactions data. We applied the four most used supervised classification algorithms. Then, we improved their performance by hyperparameters and tuning. We also performed RFECV feature selection to extract and rank the most critical variables. The experimental results represent that combining various machine learning developed algorithms in a funnel of meta-classifier can extract the highest prediction accuracy.