bluuebunny/crossref_metadata_2025_split huggingface dataset:
Who carries the baby? How lesbian couples in the Netherlands choose birth motherhood
Authors: Geerts Allison , Evertsson Marie 
Published: June 2022

AbstractObjectiveThe purpose of the study is to contribute to an understanding of the cultural and normative meaning of birth motherhood and how lesbian couples decide who carries the child.BackgroundThe decision of who carries the child is central in lesbian family‐making, carrying consequences for life after birth. Even so, it has been relatively overlooked in research. Drawing from the sociology of personal life and Park's (2013) conceptualization of monomaternalism, we study how informants consider and decide birth motherhood.MethodSemistructured interviews with both partners in 21 pregnant lesbian couples in the Netherlands were thematically analyzed.ResultsThe meaning of birth motherhood was ambivalent, linked to femininity, socially recognized motherhood, and biogenetic imaginaries. In couples where both wanted to carry, age, which carried different symbolic meanings, was a powerful tiebreaker.ConclusionOur study shows how the monomaternalist norm shapes conceptualizations of birth motherhood. Desires to experience pregnancy are strong for many. Referring to age can be a way for couples to defuse tension, but it can also be a resource drawn upon to close further negotiations.ImplicationsOur study carries implications for policy makers, health care workers, and mothers‐to‐be. Scholarly, it illuminates the ways in which motherhood, in its various forms, is perceived and recognized.
Breeding forages with climate resiliency in temperate/tropical transition zones
Authors: Quesenberry Kenneth H. , Rios Esteban F. , Kenworthy Kevin E. , Blount Ann R. , Reith Paul E. 
Published: June 2022

AbstractAs the earth's climate changes, the ability of breeders to select for traits within forage species that impart adaptability to these changes will be critical for the maintenance of grassland agricultural systems. Temperate ‐ tropical climate transition zones (between 27 and 31° N and S latitude) have proven to be ideal zones for breeding species with variable climate adaptation. Programs located in these regions have the advantage of exposure to alternating extreme warm and cold temperatures, drought and flood conditions, and a multitude of biotic pests including fungi, viruses, insects and nematodes. In this paper we will present examples of how forage breeding programs in north‐central Florida have capitalized on these distinct advantages for selection of cultivars with resiliency to changing climate variables. Breeding programs in red clover (Trifolium pratense L.) and annual ryegrass (Lolium multiflorum Lam.) will be discussed as examples for moving cool season species into warmer climates. Species attributes that contribute to climate resiliency will be identified and described. The ability to identify small changes in genetic photoperiod responses in these regions, are illustrated as an advantage when the objective is development of earlier or later maturity. Transition zones also provide suitable environments for biotic stresses, from both tropical and temperate areas, including fungal diseases, nematodes, and insects, offering desirable field environments for screening and genetic improvement.
Proteomic analysis of infected root canals with apical periodontitis in patients with type 2 diabetes mellitus: A cross‐sectional study
Authors: Loureiro Caroline , Buzalaf Marília Afonso Rabelo , Pessan Juliano Pelim , Ventura Talita Mendes Oliveira , Pelá Vinícius Taioqui , Ribeiro Ana Paula Fernandes , Jacinto Rogério de Castilho 
Published: June 2022

AbstractAimThis study aimed to quantitatively and qualitatively determine the proteomic profile of apical periodontitis (AP) in type 2 diabetes mellitus (T2DM) patients in comparison with systemically noncompromised patients and to correlate the protein expression of both groups with their biological functions.MethodologyThe sample consisted of 18 patients with asymptomatic AP divided into two groups according to the presence of T2DM: diabetic group—patients with T2DM (n = 9) and control group—systemically healthy patients (n = 9). After sample collection, the root canal samples were prepared for proteomic analysis using reverse‐phase liquid chromatography‐mass spectrometry. Label‐free quantitative proteomic analysis was performed by Protein Lynx Global Service software. Differences in protein expression between groups were calculated using t‐test (p < .05). Biological functions were analysed using the Homo sapiens UniProt database.ResultsA total of 727 human proteins were identified in all samples. Among them, 124 proteins common to both groups were quantified, out of which 65 proteins from the diabetic group showed significant differences compared with the control: 43 upregulated (p < .05) and 22 downregulated (p < .05) proteins. No significant differences in protein expression were seen for the remaining 59 proteins (p > .05). Most proteins with differences in expression were related to immune/inflammatory response. Neutrophil gelatinase‐associated lipocalin, Plastin‐2, Lactotransferrin and 13 isoforms of immunoglobulins were upregulated. In contrast, Protein S100‐A8, Protein S100‐A9, Histone H2B, Neutrophil defensin 1, Neutrophil defensin 3 and Prolactin‐inducible protein were downregulated.ConclusionsQuantitative differences were demonstrated in the expression of proteins common to diabetic and control groups, mainly related to immune response, oxidative stress, apoptosis and proteolysis. These findings revealed biological pathways that provide the basis to support clinical findings on the relationship between AP and T2DM.
Concomitant hypertrophic lichen planus and squamous cell carcinoma: Clinical features and treatment outcomes
Authors: Idriss Munir H. , Barbosa Naiara , Chang Michael B. , Gibson Lawrence , Baum Christian L. , Vidal Nahid Y. 
Published: June 2022

AbstractBackgroundPatients with hypertrophic lichen planus (HLP) and squamous cell carcinoma (SCC) diagnoses present clinicians with diagnostic and disease management challenges.ObjectiveTo better define the clinical and treatment outcomes of patients with concomitant diagnoses of HLP and SCC.Methods and MaterialsA retrospective review was performed from January 1, 2008, to July 31, 2015, at Mayo Clinic. Patients with a histologic diagnosis of HLP and SCC were included. Patient demographics, associated comorbidities, histopathologic characteristics, treatment, and outcomes were evaluated.ResultsThirty‐three patients were identified; 79% were female, and mostly the lower extremities were involved. Most of the SCCs were well‐differentiated and in situ with the majority treated with destruction or excision. There were no cases of local recurrence, metastasis, or disease‐specific death during the follow‐up period (mean 55.8 months).ConclusionPatients with diagnoses of both HLP and SCC appear to be a distinct population that is predominantly female with lesion predilection for the lower extremities. However, regardless of treatment modality or tumor size, there were no adverse outcomes. An initial trial of more conservative treatment measures with close follow‐up may be reasonable with biopsy of lesions unresponsive to conventional treatment.
Metaphylactic antimicrobial effects on occurrences of antimicrobial resistance in Salmonella enterica, Escherichia coli and Enterococcus spp. measured longitudinally from feedlot arrival to harvest in high-risk beef cattle
Authors: Long Nathan S. , Wells James E. , Berry Elaine D. , Legako Jerrad F. , Woerner Dale R. , Loneragan Guy H. , Broadway Paul R. , Carroll Jeff A. , Sanchez Nicole C. Burdick , Fernando Samodha C. , Bacon Carley M. , Helmuth Cory L. , Smock Taylor M. , Manahan Jeff L. , Hoffman Ashley A. , Hales Kristin E. 
Published: June 2022

Abstract

Aims
Our objective was to determine how injectable antimicrobials affected populations of Salmonella enterica, Escherichia coli and Enterococcus spp. in feedlot cattle.

Methods and Results
Two arrival date blocks of high-risk crossbred beef cattle (n = 249; mean BW = 244 kg) were randomly assigned one of four antimicrobial treatments administered on day 0: sterile saline control (CON), tulathromycin (TUL), ceftiofur (CEF) or florfenicol (FLR). Faecal samples were collected on days 0, 28, 56, 112, 182 and study end (day 252 for block 1 and day 242 for block 2). Hide swabs and subiliac lymph nodes were collected the day before and the day of harvest. Samples were cultured for antimicrobial-resistant Salmonella, Escherichia coli and Enterococcus spp. The effect of treatment varied by day across all targeted bacterial populations (p ≤ 0.01) except total E. coli. Total E. coli counts were greatest on days 112, 182 and study end (p ≤ 0.01). Tulathromycin resulted in greater counts and prevalence of Salmonella from faeces than CON at study end (p ≤ 0.01). Tulathromycin and CEF yielded greater Salmonella hide prevalence and greater counts of 128ERYR E. coli at study end than CON (p ≤ 0.01). No faecal Salmonella resistant to tetracyclines or third-generation cephalosporins were detected. Ceftiofur was associated with greater counts of 8ERYR Enterococcus spp. at study end (p ≤ 0.03). By the day before harvest, antimicrobial use did not increase prevalence or counts for all other bacterial populations compared with CON (p ≥ 0.13).

Conclusions
Antimicrobial resistance (AMR) in feedlot cattle is not caused solely by using a metaphylactic antimicrobial on arrival, but more likely a multitude of environmental and management factors.
Unique prediction of developmental psychopathology from genetic and familial risk
Authors: Loughnan Robert J. , Palmer Clare E. , Makowski Carolina , Thompson Wesley K. , Barch Deanna M. , Jernigan Terry L. , Dale Anders M. , Fan Chun Chieh 
Published: June 2022

BackgroundEarly detection is critical for easing the rising burden of psychiatric disorders. However, the specificity of psychopathological measurements and genetic predictors is unclear among youth.MethodsWe measured associations between genetic risk for psychopathology (polygenic risk scores (PRS) and family history (FH) measures) and a wide range of behavioral measures in a large sample (n = 5,204) of early adolescent participants (9–11 years) from the Adolescent Brain and Cognitive Development StudySM. Associations were measured both with and without accounting for shared variance across measures of genetic risk.ResultsWhen controlling for genetic risk for other psychiatric disorders, polygenic risk for problematic opioid use (POU) is uniquely associated with lower behavioral inhibition. Attention deficit hyperactivity disorder (ADHD), depression (DEP), and attempted suicide (SUIC) PRS shared many significant associations with externalizing, internalizing, and psychosis‐related behaviors. However, when accounting for all measures of genetic and familial risk, these PRS also showed clear, unique patterns of association. Polygenic risk for ASD, BIP, and SCZ, and attempted suicide uniquely predicted variability in cognitive performance. FH accounted for unique variability in behavior above and beyond PRS and vice versa, with FH measures explaining a greater proportion of unique variability compared to the PRS.ConclusionOur results indicate that, among youth, many behaviors show shared genetic influences; however, there is also specificity in the profile of emerging psychopathologies for individuals with high genetic risk for particular disorders. This may be useful for quantifying early, differential risk for psychopathology in development.
Factors associated with severity of atopic dermatitis – a Finnish cross‐sectional study
Authors: Salava A. , Salo V. , Leppänen J. , Lauerma A. , Remitz A. 
Published: June 2022

AbstractBackgroundSeverity‐associated factors in atopic dermatitis (AD) have focussed on early onset, concomitant atopic diseases, markers of Th2‐shifted inflammation and filaggrin mutations.ObjectivesTo investigate factors associated with severe AD in Finnish patients.MethodsWe conducted a single‐centre, cross‐sectional observational study with 502 AD patients aged 4.79 to 79.90 years (mean 32.08 years). Disease severity was assessed with the Rajka–Langeland severity score and EASI and associated clinical signs were evaluated. Data regarding onset, relatives, atopic and other comorbidities was gathered retrospectively. We investigated total serum IgE‐levels, a panel of filaggrin null mutations and functional variants of genes associated with skin barrier defects.ResultsFactors more frequent in severe AD included early onset (P = 0.004, 95%CI 0.000–0.024), male sex (P = 0.002, 95%CI 0.000–0.11), history of smoking (P = 0.012, 95%CI 0.000–0.024), concomitant asthma (P = 0.001, 95%CI 0.000–0.011), palmar hyperlinearity (P = 0.013, 95%CI 0.014–0.059), hand dermatitis (P = 0.020, 95%CI 0.000–0.029) and history of contact allergy (P = 0.042, 95%CI 0.037–0.096). Body mass indices (P < 0.000, 95%CI 0.000–0.011) and total serum IgE‐levels (P < 0.000, 95%CI 0.000–0.011) were higher in severe AD. No differences were observed for allergic rhinitis, allergic conjunctivitis, food allergy, peanut allergy, prick positivity, keratosis pilaris, history of herpes simplex infections, filaggrin null mutations and other gene variants.ConclusionsSeverity determinants in Finnish patients seem to be early‐onset, male sex, smoking, overweight, concomitant asthma, palmar hyperlinearity, hand dermatitis and high IgE‐levels. A sub‐typing of patients in relation to confirmed severity determinants may be useful for course prediction, prognosis and targeted AD management.
Effects of Fe3+ in sillimanite on mineral stabilities and parageneses in ultrahigh‐temperature metapelites
Authors: Wang Bin , Wei Chunjing 
Published: June 2022

AbstractBased on the nature of ferric sillimanite, an activity model for sillimanite containing Fe3+ is constructed, tested and adopted to calculate phase equilibria of pelitic compositions under ultrahigh‐temperature (UHT) conditions. The calculated P–T projections and pseudosections suggest that the incorporation of Fe3+ into sillimanite can fairly solve the current imperfectly topological match between thermodynamic calculations and synthetic experiments, especially at high oxygen fugacity. Fe3+ in sillimanite remarkably elevates the temperature to switch the parageneses of orthopyroxene + sillimanite (Opx + Sil) and sapphirine + quartz (Spr + Qz) in oxidized metapelites, with an increment around 50–70°C. The calculated compatibility diagrams show that the widely approbatory UHT‐diagnostic mineral assemblages of Opx + Sil and Spr + Qz usually occur in metapelites with high Mg/Fe2+ ratios, which depend on both bulk‐rock MgO and oxygen fugacity, whereas the metapelites with low Mg/Fe2+ ratios are characterized by the assemblage of Garnet + sillimanite (Grt + Sil) with or without spinel (Spl) in UHT conditions. Moreover, comprehensive comparisons suggest that the essential petrogenetic framework of natural UHT metapelites is mostly governed by the two metamorphic reactions of Opx + Sil = Spr + Grt and Grt + Sil = Spl + Spr.
Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia (DIPNECH): Prevalence, clinicopathological characteristics and survival outcome in a cohort of 311 patients with well‐differentiated lung neuroendocrine tumours
Authors: Hayes Aimee R. , Luong Tu Vinh , Banks Jamie , Shah Heer , Watkins Jennifer , Lim Eric , Patel Anant , Grossman Ashley B. , Navalkissoor Shaunak , Krell Daniel , Caplin Martyn E. 
Published: June 2022

AbstractIntroductionDiffuse idiopathic pulmonary neuroendocrine cell hyperplasia (DIPNECH) is considered to be a rare condition associated with lung neuroendocrine tumours (NET), and its natural history is poorly described. We aimed to assess the prevalence and clinicopathologic characteristics of DIPNECH in the lung NET population, and to investigate predictors of time‐to‐progression (TTP) and overall survival (OS).MethodsWe retrospectively identified patients diagnosed with DIPNECH between April 2005 and December 2020. Clinical data were collected from medical records. The relationship between baseline characteristics and TTP and OS was analysed using the Kaplan–Meier method. Univariate analysis was performed using the Cox proportional hazards model.ResultsOf 311 patients with well‐differentiated lung NETs, 61 (20%) had DIPNECH and were included in the study. Baseline demographics described 95% female, 59% never smokers and mean body mass index 34.4 kg m‐2; 77% were typical carcinoids (TC), 13% atypical carcinoids (AC), and 10% both TC and AC (multicentric). At presentation, 54% of patients were asymptomatic. Multicentric NETs were demonstrated in 16 (26%) on histopathology, and a further 32 (52%) had synchronous NETs suggested on imaging (multiple nodules ≥ 5 mm). Seven (11%) patients developed metastases and the median OS from time of first metastasis was 37 months. AC histopathology and NET TNM stage ≥ IIA were associated with poorer TTP and OS. Of the DIPNECH cohort, the 15‐year survival rate was 86%.ConclusionsDIPNECH may be more prevalent in the lung NET population than previously appreciated, especially in women. Although our results confirm that DIPNECH is predominantly an indolent disease associated with TC, 23% developed AC and these patients may warrant closer observation.
Chemical, radiological and microbiological characterization of a drinking water source: a case study
Authors: Grujović M. Ž. , Mladenović K. G. , Marković S. M. , Đukić N. H. , Stajić J. M. , Ostojić A. M. , Zlatić N. M. 
Published: June 2022

AbstractThis study examined water samples from a local stream in Central Serbia, which was consumed as drinking water. The chemical parameters (chemical oxygen demand, COD; pH, total concentration of dissolved substances and electrical conductivity), the concentration of major, trace and radioactive elements in the water as well as the content of those from the environment, were examined. In addition, the microbiological quality of the water was inspected. The water samples were acidic (pH from 5·27–5·69) and COD ranged in upper permissible limits (up to 6·25 mg O2 l−1 [WR]). The concentrations of major, trace and radioactive elements, including radon, were below maximum contaminant levels. The water contained a higher number of total coliform bacteria (TCB) than it was allowed (˃10 colony-forming units (CFU) in 100 ml of water) as well as enterococci and Escherichia coli. The characterization of the isolated bacteria indicated that two isolates demonstrated proteolytic activity, while full antibiotic resistance was not detected. The isolates showed moderate to strong ability to produce biofilm, while the isolates of E. coli were nonpathogenic. The results indicated that examined water samples were not microbiologically and chemically safe, therefore, the usage of analysed water was not recommended as a water supply. Further research needs to include more frequent monitoring in order to propose measures for the improvement of the water quality and prevention of health risks for consumers.
Single‐cell analysis reveals immune cellular components in odontogenic keratocysts
Authors: Man Qi‐Wen , Li Rui‐Fang , Liu Jian‐Feng , Zhao Yi , Liu Bing 
Published: June 2022

AbstractObjectivesVarious types of cells comprising a complex and diverse cell population are required for the biological activities of odontogenic keratocyst (OKC). Immune and non‐immune cells collaborate via cytokine‐ or chemokine‐mediated communication and direct cell–cell interactions. This study aimed to characterize the immune ecosystem and understand the potential chemotactic role of OKC fibroblasts in immune cell migration.Materials and MethodsMass cytometry of 41 markers was employed for the classification of OKC cells from six OKC samples. Immunofluorescence staining and single‐cell RNA sequencing (GSE176351) were used for the detection of fibroblast subpopulations. Enzyme‐linked immunosorbent assay and immunofluorescence staining were employed for chemokine detection in hypoxia‐ and/or HIF‐1α inhibitor‐treated OKC fibroblasts and tissues. Chemotaxis assay was employed to determine the chemotactic effect of fibroblasts via co‐culture with peripheral blood mononuclear cells. A cell communication network was constructed based on the single‐cell RNA sequencing data.ResultsThe characterization of the immune cell types of OKC evidenced the enrichment of macrophages, neutrophils and B cells. The majority (41.5%) of fibroblast subsets consisted of chemokine ligand‐enriched myofibroblasts. The activation of the HIF‐1α signaling pathway in fibroblasts was associated with chemokine release. The chemokines released by OKC fibroblasts remarkably promoted the migration of peripheral blood mononuclear cells in the co‐culture system. Close interactions between myofibroblasts and immune cells were validated by cell–cell interaction analysis. Increased RANKL expression was detected in OKC fibroblasts in the co‐culture system with peripheral blood mononuclear cells.ConclusionsOur results provided deep insights into the immune ecosystem and highlighted the potential chemotactic effects of chemokine‐enriched myofibroblasts within OKCs. The close interaction between immune cells and fibroblasts demonstrated in this study may be responsible for the osteoclastogenic effects of OKC fibroblasts.
Oral leukoplakia classification and staging system with incorporation of differentiated dysplasia
Authors: Brouns Elisabeth R. , Evren Ilkay , Wils Leon J. , Poell Jos B. , Brakenhoff Ruud H. , Bloemena Elisabeth , de Visscher Jan G. A. M. 
Published: June 2022

AbstractObjectivesA classification and staging system for oral leukoplakia (OL) was introduced to promote uniform reporting. In this system, size and the histopathologic diagnosis are assessed and combined in a staging system. The various stages could be predictive for malignant transformation of OL. Differentiated dysplasia (DD) was recently recognized as an important architectural pattern of dysplasia and is highly associated with malignant transformation (MT) of OL. In the present study, DD was incorporated in the OL‐system. The aim of the present study was to test the adapted system on a cohort of patients with OL.Patient and methodsThe group consisted of 140 patients. The size, absence or presence and degree of classic dysplasia (CD) and DD were incorporated into the OL‐system.ResultsIn 31/140 patients, MT occurred. Size was not statistically significant with MT (p = 0.422). The presence of dysplasia was predictive for MT (p = 0.003), whereby severe CD and DD were highly statistically significant for MT (p = 0.008). Stage IV was statistically significant for MT (p = 0.011).ConclusionsThe present study emphasizes the value of the slightly modified OL‐system with incorporation of DD in uniform reporting of OL and the value in predicting MT.
Visual observation of extraction of a Micra leadless pacemaker from a human cadaver
Authors: Bonner Matthew , Hilpisch Kathryn , Wika Kent , Mesich Mary Lauren , Harris Megan , White Troy 
Published: June 2022

AbstractIntroductionIn this article we present the extraction of a Micra from a human cadaver implanted 3 years previously with both visual and X‐ray imaging taken during the removal.MethodsA Micra pacemaker was extracted from a human cadaver with endoscopy and fluoroscopy using a Micra delivery tool. Histological analysis was performed on slices from the tissue surrounding the Micra.ResultsThe fully encapsulated Micra was easily retrieved with a maximum force of 1.9 pounds.ConclusionsEven though the Micra was implanted almost 3 years previously, the snaring and extraction of the Micra was performed relatively easily and with minimal force required.