bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of multiple irradiations and Reference Materials as comparators in quality control of neutron activation analysis data of biological samples
Authors: GARG AMAR , Singh Vivek , Choudhury R. P. 
Published: May 2022

Abstract
We propose that several different reactor irradiation times followed by assaying of activity for differential counting periods may be employed for quality control (QC) of neutron activation analysis (NAA) data of biological samples. It is also recommended that three to four Reference Materials (RMs) of similar matrix but from different agencies such as National Institute of Standards and Technology (NIST, USA), International Atomic Energy Agency (IAEA, Vienna), Institute of Nuclear Chemistry and Technology (INCT, Poland), National Institute of environmental Studies (NIES, Japan) including a synthetic multielemental primary standard should always be analyzed simultaneously along with the samples. Finally, the mean ± σ values so obtained may be considered as more reliable after statistical analysis. We report our data for 20 elements in two candidate RMs Corn flour (INCT-CF-3) and Soya Bean flour (INCT-SBF-4) from the INCT, Poland where Z-score values are in reasonable range of certified values.
The impact of heat treatments on the content of beneficial substances in the most consumed vegetables in Slovakia
Authors: Lidiková Judita , Čeryová Natália , Šnirc Marek , Bobko Marek , Musilová Janette , Vollmannová Alena , Bobková Alica , Bojňanská Tatiana 
Published: May 2022

Abstract
In the present study, we investigated the effects of heat treatment (boiling in water, steaming) on ​​changes in the content of vitamin C, total polyphenols, and changes in antioxidant activity in garlic, onion, pepper, potatoes, and tomatoes. In the obtained extracts of the mentioned raw and heat-treated vegetables, we determined the content of vitamin C by HPLC, the content of total polyphenols by Folin- Ciocalteau colorimetric method, as well as the antioxidant activity (DPPH assay, ABTS assay, FRAP assay). The obtained results showed that the content of vitamin C, as well as the content of total polyphenols in the monitored vegetables after heat treatment (cooking in water), was significantly reduced (P < 0.05) compared to the content of these bioactive substances in raw vegetables. A slight decrease in vitamin C and total polyphenol content was recorded in the steamed samples. This decrease was not statistically significant for all these crops. Determination of antioxidant activity again showed a more significant decrease in antioxidant activity values (P < 0.05) (DPPH, ABTS, FRAP) after boiling in water than after steaming. The heat treatment has a significant impact on the change in the content of monitored bioactive substances in the analyzed vegetables. Based on our achieved results, steaming appears to be a more gentle way in terms of preserving natural antioxidants, which can significantly contribute to the elimination of oxidative stress in our organism.
Looking Beyond the Cytogenetics in Haematological Malignancies- Decoding the Role of Tandem Repeats in DNA Repair Genes
Authors: Bhattacharya Priyanjali , Patel Trupti 
Published: May 2022

Abstract
Background - In cancer research, one of the most significant findings was to characterize the DNA repair deficiency as carcinogenic. Amongst the various repair mechanisms, mismatch repair (MMR) and direct reversal of DNA damage (DRD) systems are designated as multilevel safeguards in the human genome. Defects in these elevate the rate of mutations and results in dire consequences like cancer. Of the several molecular signatures in human genome, tandem repeats (TRs) appear at various frequencies in the exonic, intronic, and regulatory regions of the DNA. Hypervariability among these repeats in the coding and non-coding regions of the genes is well characterized for solid tumours, but its significance in haematologic malignancies remains to be explored. The purpose of our study was to elucidate the role of nucleotide repeat instability in the coding and non-coding regions of 10 different repair genes in myeloid and lymphoid cell lines compared to the control samples. Methods and Results - We selected MMR deficient extensively studied microsatellite instable colorectal cancer (HCT116), and MMR proficient breast cancer (MCF-7) cells along with underemphasized haematologic cancer cell lines to decipher the hypermutability of tandem repeats. A statistically significant TR variation was observed for MSH2 and MSH6 genes in 4 and 3 of the 6 cell lines respectively. KG1 (AML) and Daudi (Burkitt’s lymphoma) were found to have compromised DNA repair competency with highly unstable nucleotide repeats. Conclusion - Taken together, the results suggest that mutable TRs in intronic and non-intronic regions of repair genes in blood cancer might have a tumorigenic role. Since this is a pilot study on cell lines, high throughput research in large cohorts can be undertaken to reveal novel diagnostic markers for unexplained blood cancer patients with normal karyotypes or otherwise with karyotypic defects.
Is there a benefit of the robotic technique in patients undergoing transthoracic hybrid esophagectomy ?
Authors: Reinstaller Therese , Daniela Adolf , Lorenz Eric , Croner Roland S , Benedix Frank 
Published: May 2022

Abstract
Purpose Minimally invasive en-bloc esophagectomy is associated with a reduction of postoperative morbidity. This was demonstrated for both total minimally invasive and hybrid esophagectomy. However, little is known about any benefits of robotic assistance compared to the conventional minimally invasive technique, especially in hybrid procedures.Methods For this retrospective study, all consecutive patients who had undergone elective esophagectomy with circular stapled intrathoracic anastomosis using the open and the minimally invasive hybrid technique at the University hospital Magdeburg, from January 2010 to March 2021 were considered for analysis.Results In total, 137 patients (60.4%) had undergone open esophagectomy. In 45 patients (19.8%), the laparoscopic hybrid technique and in 45 patients (19.8%), the robot-assisted hybrid technique were applied. In propensity score matching analysis comparing the open with the robotic hybrid technique, significant differences were found in favor of the robotic technique (postoperative morbidity, p < 0.01; hospital length of stay, p < 0.01; number of lymph nodes retrieved, p = 0.048). In propensity score matching analysis comparing the laparoscopic with the robotic hybrid technique, a significant reduction of the rate of postoperative delayed gastric emptying (p = 0.02) was found for patients who had undergone robotic esophagectomy. However, the operation time was significantly longer (p < 0.01).Conclusions En-bloc esophagectomy using the robotic hybrid technique is associated with a significant reduction of postoperative morbidity and of the hospital length of stay when compared to the open approach. However, when compared to the laparoscopic hybrid technique, only few advantages could be demonstrated.
Differences in L-arginine metabolism and asthma morbidity among asthma patients with and without obstructive sleep apnea
Authors: Althoff Meghan D. , Jimenez Guillermo , Peterson Ryan , Jin Ying , Grasemann Hartmut , Sharma Sunita , Federman Alex D. , Wisnivesky Juan P. , Holguin Fernando 
Published: May 2022

Abstract
Background
Imbalance in L-arginine and nitric oxide (NO) metabolism has been implicated in the pathophysiology of asthma and obstructive sleep apnea (OSA), and both diseases impact the other’s morbidity. We sought to determine whether L-arginine/NO metabolism differs between adults with asthma with or without comorbid OSA, and its association with asthma morbidity.
Methods
This is a cross-sectional study of 322 adults with asthma recruited in Denver, CO and New York City, NY. Data were collected on OSA status, spirometry, and metrics of asthma control and morbidity. L-arginine metabolites were quantified in patient serum. Bivariate analyses and multiple regression were performed to determine differences between L-arginine metabolism, OSA and association with asthma morbidity.
Results
Among the 322 participants, 92 (28.5%) had OSA. The cohort was 81.6% female, 23.4% identified as Black and 30.6% as Latino. Patients with asthma and OSA had significantly higher serum concentrations of NO synthase inhibitor asymmetric dimethylarginine (ADMA) (p-value = 0.019), lower L-arginine to ornithine ratios (p-value = 0.003), and increased ornithine (p-value = 0.001) and proline levels (p-value < 0.001) compared to those without OSA. In adjusted models, OSA was associated with worse asthma control, mean difference in asthma control questionnaire of 0.36 (95% confidence interval [CI]: 0.06 to 0.65), and asthma quality of life questionnaire, mean difference: -0.53 (95% CI: -0.85 to -0.21), after adjusting for relevant covariates including body mass index and L-arginine metabolites.
Conclusions
Adults with asthma and OSA had increased ADMA, an inhibitor of nitric oxide synthase, and greater metabolism of L-arginine via the arginase pathway compared to those with asthma alone, indicating a possible shared pathophysiological mechanism of these diseases.
Transgenerational inheritance of paternal DNA damage by linker histone H1-mediated DNA repair restriction
Authors: Schumacher Björn , Wang Siyao , Meyer David 
Published: May 2022

Abstract
How paternal exposure to ionizing radiation (IR) affects genetic inheritance and disease risk in the offspring has been one of the most long-standing questions in radiation biology. While in humans, 80% of transmitted mutations arise in the paternal germline, the transgenerational effects of ionizing radiation (IR)-exposure has remained controversial and the mechanisms elusive. Here, we show that in sex-separated C. elegans strains paternal, but not maternal, IR-exposure leads to a transgenerational embryonic lethality. Offspring of irradiated males displayed various genome instability phenotypes, including DNA fragmentation, chromosomal rearrangement, and aneuploidy. We determined that paternal DNA double strand breaks (DSBs) are repaired by maternally provided error-prone polymerase-theta mediated end joining (TMEJ). Mechanistically, we show that depletion of a human histone H1.0 ortholog, HIS-24, or the heterochromatin protein, HPL-1, could significantly reverse the transgenerational embryonic lethality. Removal of HIS-24 or HPL-1 reduced the heterochromatin marker histone 3 lysine 9 di-methylation (H3K9me2) and allowed the employment of the error-free Homologous Recombination Repair (HRR) in the germline of the F1 of IR-treated P0 males, consequently improving the viability of the F2 generation. Our work establishes the mechanistic underpinnings of transgenerational consequences of paternal radiation exposure on the health of offspring, which may lead to congenital disorders and cancer in humans.
Thermal error prediction of ball screw feed system based on inverse heat transfer analysis
Authors: Li Zhen-jun , Tan Zhi , Chen Ye , Lu Ze-chen , Fan Yu-chuan 
Published: May 2022

Abstract
The thermal error of the ball screw feed system will reduce the positioning accuracy of the machine tool, and the main reason for the thermal error is the friction heat generated by bearings and screw nut in the feed system. Therefore, a time-varying thermal error prediction method of the ball screw feed system based on the inverse method is proposed in this paper. Based on data-driven and dynamic thermal network model, the heat source estimation method of ball screw feed system under unsteady thermal effect is established, and the thermodynamic equilibrium equation is deduced into explicit form for heat source load identification. Aiming at the common matrix ill-conditioned problem of load identification, the regularization algorithm is used to identify the heat source load, and the optimal selection method of regularization parameters is proposed based on Monte Carlo algorithm. Using the collected temperature experiment data and the position data of the moving nut, the dynamic heat source load, temperature field and thermal error of the feed system under the actual working condition are predicted and analyzed by using the inverse method. Finally, the accuracy and effectiveness of the prediction method are verified by experiment. The inverse method proposed in this paper has great application potential for the prediction and estimation of heat source and temperature field of machine tools and various mechanical structures.
Effect of supplementing hydrolysable tannins extract on milk proteins and productive performance of early lactating Nili Ravi buffaloes
Authors: Raza Mohsin , Pasha Talat Naseer , Saima  , Azam Burhan e , Shahid Muhammad Qamar , Ahmed Saeed , Ali Usman , Ahmad Sohail , Akhtar Muhammad Uzair 
Published: May 2022

Abstract
Supplementation of tannin extract with proper dosage can increase the protein precipitation, decreasing the ruminal protein losses, ensuring the availability of nutrient and their utilization in the body. Nine early lactating Nili Ravi buffaloes were selected and divided into 3 groups. Buffaloes were randomly assigned to 1of 3 treatments in a 3× 3 Latin square design. Treatments were 0, 15, and 30 g/d per animal supplemental levels of tannin extract. The period length of each treatment was 21 days. All the buffaloes were fed similar basal diet and tannin extract was mixed in concentrate. Dry matter intake and milk protein content increased linearly with increasing tannin supplementation. Nitrogen intake, apparent digestibility of nitrogen, and milk nitrogen efficiency were similar among treatments. Blood urea nitrogen and milk urea nitrogen decreased with tannin supplementation indicating reduced protein degradation. Manure scoring increased but feed efficiency decreased linearly with increasing level of tannin supplemental level. Overall, it is observed that tannin supplementation decreases blood and milk urea nitrogen and increases milk protein content and tends to increase dry matter intake.
Biotransformation of testosterone by the filamentous fungus Penicillium pinophilum
Authors: Mehmannavaz Maryam , Nickavar Bahman 
Published: May 2022

Abstract
The microbial biotransformation is a robust procedure in developing steroids and fungi are practical tools in this process therefore, the fungal modification of testosterone by Penicillium pinophilum was investigated. The three prominent metabolites, including 14α-hydroxyandrost-4-en-3,17-dione (II), 14α-hydroxytestosterone (III), and 11α-hydroxytestosterone (IV), were isolated and characterized by chromatographic and spectroscopic methods. The time course profile showed that the content of the metabolites II and III began to decrease after 24 h and 96 h, respectively. In comparison, the content of the metabolite IV remained stable after 24 h. In-silico studies showed that the probability of binding to the androgen receptor remains high for all three metabolites. However, the probability of binding to the estrogen receptors α and β increased for metabolite IV but decreased for metabolite III. Penicillium pinophilum as a potentially viable biocatalyst could hydroxylate C-11α and C-14α positions and oxidize the C-17β hydroxyl group to 17-ketone in testosterone molecule.
Ultrastructure of the fertilized egg envelope from Poropanchax normani, Poeciliidae, Teleostei
Authors: Kim Dong Heui 
Published: May 2022

Abstract
We examined the morphology of fertilized egg and fine structure of fertilized egg envelopes of Norman’s lampeye, Poropanchax normani belongs to Poeciliidae using light and electron microscopes. The fertilized eggs with narrow perivitelline space were spherical, demersal, and have small oil droplets in the vitelline membrane. And a bundle of adhesive filaments was on one side of fertilized egg. The size of fertilized egg was about 1.49 ± 0.07 mm (n = 30) and length of a bundle of adhesive filaments with very high elasticity was about 1–3 mm. The outer surface was smooth. The adhesive filaments are distributed around the egg envelope and each adhesive filaments are joined together to form a long bundle. The around the micropyle was a peak shape with several straight wrinkles. But accurate structure of micropyle could not be observed because it is in too deep. Also the total thickness of the egg envelope was approximately12.5-14.5 µm and the egg envelope consisted two layered, an outer electron dense layer and an inner lamellar layer consisting of 10 layers of differing thickness. Collectively these morphological characteristics of fertilized egg and fine structures of around micropyle, outer surface, adhesive structure consisting adhesive filaments and section of fertilized egg envelope are showed species specificity.
The mechanisms of ion chanels involved in developmental neurotoxicity due to anesthetic exposure
Authors: Zeng Shuang , Zhu Ruilou , Fu Ningning , Li Ningning , Zhang Jiaqiang 
Published: May 2022

Abstract
General anesthesia is an important clinical treatment drug.General anesthesia is the result of the mixed interaction between a variety of nonspecific drugs and multiple targets in the nervous system. Preclinical studies have shown that cognitive impairment and behavioral abnormalities in rodents persist into adulthood after exposure to anesthesia, revealing the potential neurotoxicity of anaesthetic drugs.Studies have shown that neonatal rats exposed to sevoflurane increase the concentration of Cl− in neurons, leading to the depolarization of GABA receptor and neurotoxicity, which eventually leads to the impairment of spatial learning and memory in rats, indicating that anesthetics may produce neurotoxicity through ion channels, but the specific mechanism is not clear.So far, ion channel has been identified as one of the molecular targets of general anesthetics.General anaesthesia drugs have been shown to affect sodium, potassium and calcium channels.Changes in these ion channels can cause neurotoxicity that can affect the nervous system.In this paper, the effects of general anesthetics on nervous system were discussed from the changes of ion channels.
Grandmaternal smoking during pregnancy and grandchild health: A systematic review and meta-analysis
Authors: Zhang Guo-Qiang , Luo Ying , Zhang Hui-Hua , Li Zhong-Yue , Kankaanranta Hannu , Wennergren Göran , Sheikh Aziz , Nwaru Bright 
Published: May 2022

Abstract
Background
Maternal smoking during pregnancy is associated with a host of detrimental effects on the exposed child. However, emerging evidence suggests that the adverse effects of smoking during pregnancy may be transmitted across generations. We conducted a systematic review to comprehensively summarize evidence on the association between grandmaternal smoking during pregnancy and health outcomes in the grandchild.
Methods
We searched MEDLINE, EMBASE, and 10 other databases from inception to March 02, 2021, updated on October 18, 2021, to identify analytic epidemiologic studies (prospective or retrospective cohort and case-control design) investigating the association between grandmaternal smoking during pregnancy and multiple health outcomes in the grandchild in all countries and settings. Three investigators independently screened records, extracted data, and assessed study quality using the Effective Public Health Practice Project (EPHPP) tool. Random-effects robust variance estimation was used to combine effect estimates whenever possible, separately for maternal and paternal line.
Results
Twenty-four reports from 15 unique studies with 23 health outcomes and 231,478 grandchildren were included. The overall study quality was moderate. In maternal line, grandmaternal smoking during pregnancy was associated with an increased risk of asthma (seven studies, 148,527 grandchildren, risk ratio (RR) 1.10, 95% CI 1.02 to 1.18) and higher birth weight (four studies, 19,478 grandchildren, mean difference (g) 40.04, 95% CI 22.60 to 57.49) in the grandchild. There was suggestive evidence that maternal grandmaternal smoking was associated with increased risks of acute lymphoblastic leukaemia, any cancer, autism, and attention deficit hyperactivity disorder, decreased risks of early-onset myopia and small birth weight for gestational age, and higher birth length and body mass index at birth. In paternal line, grandmaternal smoking during pregnancy was not associated with asthma (four studies, 41,069 grandchildren, RR 1.01, 95% CI 0.85 to 1.19), but there was suggestive evidence for lower intelligence quotient scores and decreased risks of early-onset myopia and intolerance to loud sound.
Conclusions
The effects of maternal smoking during pregnancy may not be limited to the exposed child, but be transmitted to the grandchild, possibly through germline epigenetic inheritance. Further public health efforts are warranted to strengthen smoking cessation programs for pregnant women.
Workplace wellness programs for working mothers: a systematic review
Authors: Ernawati  , Mawardi Fitriana , Roswiyarni  , Melissa  , Wiwaha Guswan , Tiatri Sri , Hilmanto Dany 
Published: May 2022

Abstract

Background and objectives: This systematic review aimed to uncover the evidence and benefits of employers’ commitment to delivering workplace wellness programs for working mothers.Methods: The articles published in PubMed, Embase, Scopus, and AgeLine databases between 2017 and 2021 were searched to evaluate the workplace wellness programs for working mothers with at least one resultant wellness or wellbeing (e.g., physical health, less stress, mental health, burnout, depression, smoking, bullying, alcohol consumption, overweight), work-life balance outcome, or job satisfaction.Results: Four studies that met the criteria were retrieved from databases. They showed some effective workplace wellness programs that can reduce depression, stress, and burnout, improve mental health, healthy behaviours, work-family balance and work-life balance. Working mothers participating in a workplace wellness program generally gain some benefits; one of which is reduced stress typically related to childcare, economic, and personal health issues.Conclusions: The implementation of workplace wellness programs for working mothers showed positive effects on their health problems and health costs. These four studies revealed that workplace wellness programs specifically designed for working mothers can lead to time efficiency by holding the programs in or near the workplace and implementing them during the workdays. This greatly suits the conditions of many working mothers whose limited time and energy to balance the household, family and work tasks.