bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface Polarity Regulation by Relieving Fermi‐Level Pinning with Naphthalocyanine Tetraimides toward Efficient Perovskite Solar Cells with Improved Photostability
Authors: Zhou Qin , Cai Chunsheng , Xiong Qin , Zhang Zilong , Xu Jianbin , Liang Lusheng , Wang Shibo , Sun Weihai , Yuan Zhongyi , Gao Peng 
Published: June 2022

AbstractIn perovskite solar cells (PSCs), defective perovskite grain boundaries (GBs) and/or surface due to photo‐excitation and the resulted suboptimal carrier dynamics at the perovskite/charge transport layer, have largely limited further performance enhancement and aggravated the PSCs instability. Fundamentally preventing the formation of these trap‐states through a simple and efficient approach is thus critical to the enhancement of both device performance and stability. Herein, a novel semiconductive silicon naphthalocyanine derivative (Cl‐SiNcTI) to reduce the deep level trap states at the GBs and the surface of perovskite film is successfully employed via a newly proposed photon‐relaxation mechanism. The resulting benign p‐type surface polarity and suppressed non‐radiation recombination lead to improved charge transport in bulk perovskite and at the perovskite/spiro‐OMeTAD interface. With a synergistic contribution of the Cl‐SiNcTI and 2‐(2‐Fluorophenyl)ethylamine iodide (oFPEAI), a 24.30% efficiency is achieved in a single cell with excellent operational stability. Moreover, under steady‐state light illumination, 93% power output compared to its initial state can still be maintained after 250 h of continuous operation.
TTC5 syndrome: Clinical and molecular spectrum of a severe and recognizable condition
Authors: Musante Luciana , Faletra Flavio , Meier Kolja , Tomoum Hoda , Najarzadeh Torbati Paria , Blair Edward , North Sally , Gärtner Jutta , Diegmann Susann , Beiraghi Toosi Mehran , Ashrafzadeh Farah , Ghayoor Karimiani Ehsan , Murphy David , Murru Flora Maria , Zanus Caterina , Magnolato Andrea , La Bianca Martina , Feresin Agnese , Girotto Giorgia , Gasparini Paolo , Costa Paola , Carrozzi Marco 
Published: June 2022

AbstractBiallelic mutations in the TTC5 gene have been associated with autosomal recessive intellectual disability (ARID) and subsequently with an ID syndrome including severe speech impairment, cerebral atrophy, and hypotonia as clinical cornerstones. A TTC5 role in IDs has been proposed based on the physical interaction of TTC5 with p300, and possibly reducing p300 co‐activator complex activity, similarly to what was observed in Menke‐Hennekam 1 and 2 patients (MKHK1 and 2) carrying, respectively, mutations in exon 30 and 31 of CREBBP and EP300, which code for the TTC5‐binding region. Recently, TTC5‐related brain malformation has been linked to tubulinopathies due to the function of TTC5 in tubulins' dynamics. We reported seven new patients with novel or recurrent TTC5 variants. The deep characterization of the molecular and phenotypic spectrum confirmed TTC5‐related disorder as a recognizable, very severe neurodevelopmental syndrome. In addition, other relevant clinical aspects, including a severe pre‐ and postnatal growth retardation, cryptorchidism, and epilepsy, have emerged from the reversal phenotype approach and the review of already published TTC5 cases. Microcephaly and facial dysmorphism resulted in being less variable than that documented before. The TTC5 clinical features have been compared with MKHK1 published cases in the hypothesis that clinical overlap in some characteristics of the two conditions was related to the common p300 molecular pathway.
PaO2 / FiO2 ratio responsiveness to prone positioning in intubated patients with severe COVID‐19: a retrospective observational study
Authors: Kawakami Akiko , Yamakawa Kazuma , Nishioka Daisuke , Ota Koshi , Kusaka Yusuke , Umegaki Osamu , Ito Yuri , Takasu Akira 
Published: June 2022

AimProne positioning of coronavirus disease 2019 (COVID‐19) patients could improve oxygenation. However, clinical data on prone positioning of intubated COVID‐19 patients are limited. We investigated trends of PaO2 / FiO2 ratio values in patients during prone positioning to identify a predictive factor for early detection of patients requiring advanced therapeutic intervention such as extracorporeal membrane oxygenation (ECMO).MethodsThis retrospective, observational cohort study was undertaken between April 2020 and May 2021 in a tertiary referral hospital for COVID‐19 in Osaka, Japan. We included intubated adult COVID‐19 patients treated with prone positioning within the first 72 h of admission to the intensive care unit and followed them until hospital discharge or death. Primary outcomes were in‐hospital mortality and escalation of care to ECMO. We used unsupervised k‐means clustering modeling to categorize COVID‐19 patients by PaO2 / FiO2 ratio responsiveness to prone positioning.ResultsThe final study cohort comprised 54 of 155 consecutive severe COVID‐19 patients. Three clusters were generated according to trends in PaO2 / FiO2 ratios during prone positioning (cluster A, n = 16; cluster B, n = 24; cluster C, n = 14). Baseline characteristics of all clusters were almost similar. Cluster A (no increase in PaO2 / FiO2 ratio during prone positioning) had a significantly higher proportion of patients placed on ECMO or who died (6/16, 37.5%). Numbers of patients with ECMO and with in‐hospital death were significantly different between the three groups (p = 0.017).ConclusionIn Japanese patients intubated due to COVID‐19, clinicians should consider earlier escalation of treatment, such as facility transfer or ECMO, if the PaO2 / FiO2 ratio does not increase during initial prone positioning.
Polymeric films prepared from starch and a crosslinker extracted from avocado seeds
Authors: Castro‐García Perla Guadalupe , Vasquez‐Garcia Salomon Ramiro , Flores‐Ramirez Nelly , Rico Jose Luis , Abdel‐Gawwad Hamdy Ahmed , García‐González Leandro , Domratcheva‐Lvova Lada , Fernández‐Quiroz Daniel 
Published: June 2022

AbstractThe avocado seeds, considered as waste, were used for the extraction of oil rich in pentosans and starch. The avocado starch (AS) and a commercial starch (CS) are then used for the formation of polymeric films by casting. Avocado oil (AO, as natural crosslinker) and ethylene glycol (EG) are utilized as crosslinkers to modify the properties of the films. All polymeric structures are characterized by physical inspection, FTIR, TGA‐DSC, and water‐insoluble fraction. The results indicate that the incorporation of AO and EG augments the thermal stability by decreasing the degradation rates compared to that of the unmodified AS and CS polymeric films. In addition, the degradation temperature of the polymeric films is modified by the addition of crosslinkers. The degradation temperature of AS, AS‐AO, AS‐EG, were 300, 286, and 306°C, accordingly, whereas those for CS films are 314, 314.5, and 307.7°C for CS, CS‐AO, and CS‐EG, respectively. Our study demonstrates that natural wastes could be useful and increase the profits of the avocado processing industries.
The impact of COVID‐19 on autism research: A cross‐sectional analysis of discontinued or suspended clinical trials
Authors: Neale Monika , Landers Elizabeth , Sajjadi Nicholas B. , Mazur‐Mosiewicz Anya , Hartwell Micah 
Published: June 2022

AbstractDue to uncertainties associated with the COVID‐19 public health crisis, several clinical trials had to be withdrawn or postponed. Our investigation aimed to assess the rate of discontinuation of clinical trials focusing on Autism Spectrum Disorder. Of the 197 registered trials included in our systematic review, 15 (7.6%) were discontinued, with nearly half of these explicitly citing COVID‐19 as their reason for discontinuation. Pharmacological trials were six times more likely to be discontinued during the pandemic than non‐pharmacological studies. The difference between the likelihood of discontinuation was statistically significant (OR: 6.13; 95% CI: 1.22–30.71). There was no evidence of association between funding source and reasons for discontinuation. Limitations, along with implications for future trials are discussed.Lay SummaryWe investigated the impact of the COVID‐19 pandemic on the discontinuation rate of autism clinical trials. We found that drug trials were six times more likely to be discontinued during the pandemic compared to behavioral, diagnostic, and nutritional trials. The overall discontinuation rate was notably lower in autism clinical trials than in other areas of medical research. We recommend an examination of the methodology of the continued autism trials to assess their applicability in other fields.
Bioinformatics‐based and molecular docking study on the mechanism of action of Galla chinensis in the treatment of diabetic foot ulcers
Authors: Fan Shuyuan , Xu Yue , Qiu Feng , Liu Jing , Pan Taowen , Li Kun , Li Bin , Diao Yunpeng 
Published: June 2022

AbstractBased on transcriptome sequencing and molecular biology, the active ingredient of Galla chinensis in the treatment of diabetic foot ulcers was identified, and its mechanism of action was analyzed.
A study of the THP‐1 cell line as the potential biologics production platform with the emphasis on serum‐free media substitution for economic expediency
Authors: Kimiz‐Gebologlu Ilgin , Demirden S. Furkan , Oncel Suphi S. 
Published: June 2022

AbstractCell cultures are frequently preferred in the industrial production of high value‐added biopharmaceuticals on a large scale. In the production of biopharmaceuticals, the selection of the appropriate cell line, the cell culture medium, and the culture conditions are very important. In medical products to be offered for human use, authorized institutions do not allow the use of serum which increases the culture yield, added to the culture. For this reason, the cell lines to be used must be adapted to the serum‐free medium. In this study, first, the direct and gradual adaptation of the THP‐1 cell line, which has a high potential for use in biopharmaceutical production, to a locally produced chemically defined serum‐free medium was carried out. Then, a comparison of the production efficiency in the shake flasks with the commercially available two different serum‐free media was performed. Finally, for the first time, THP‐1 cells were produced in spinner flasks and stirring tank bioreactor to simulate the large scale within the scope of this work.
Proposition of a structural model for business value creation based on circular business models, innovation, and resource recovery in the pet industry
Authors: Kuzma Edson , Sehnem Simone 
Published: June 2022

AbstractBased on the assumptions of circular economy, resource recovery, and innovation, the aim of this research is to analyze the relationship between innovation constructs, resource recovery practices, Circular Economy Business Models (CEBMs), and value creation in a fast‐growing emerging economy. A set of empirical data from 443 companies in the pet industry were used. In terms of methodology, the study was processed using the SPSS software, using Structural Equation Modeling, with manipulation of primary data. CEBMs demand greater engagement, internalization of initiatives, and synergy with the operating ecosystem of the pet industry. Innovation is present but has the potential for expansion through adherence to the innovation ecosystem and its technological package, partnerships, and relationships so that companies can scale up and create value with the support of resource recovery practices. The results of this study can be useful for companies in the pet industry, as it allows the identification of areas and practices that require their attention to improve organizational performance and create value for the business based on initiatives supported by the assumptions of circular economy and sustainability.
Advancing engineering education: Using the three‐phase methodology to teach IoT
Authors: Assumpção Raphael M. , Chaves Pedro R. , Ferreira Luiz C. , Cardieri Paulo , Branquinho Omar C. , Fruett Fabiano 
Published: June 2022

AbstractThe multidisciplinary aspect of Internet of Things (IoT) requires teaching approaches that can support this characteristic. Research has shown that inductive teaching methods can contribute to deeper learning. The development of the three‐phase methodology (TpM), an approach that guides the many disciplines involved in the creation of IoT solutions, precipitated the development of strategies for its implementation. To test these strategies, we propose a new learning environment that uses the TpM in a postgraduate course in IoT where students are introduced to the concepts required to design, build, and test IoT solutions. The adoption of the TpM combined with extensive experimentation can produce a learning environment that encourages students’ active participation in the activities and facilitate the retention of the concepts learned. To evaluate the effectiveness of the proposed approach, we compared the results of an earlier edition of the course (2019), when the approach was not used, to two more recent ones (2020 and 2021) when it was. The same syllabus and rubrics were used in all three editions of the course. The final group projects’ grades and satisfaction surveys were used to evaluate the students’ performance and motivation. Findings indicate that the 2020 and 2021 classes performed better than the 2019's, as their final group project grades were considerably higher, indicating that the approach has an impact on the development of students’ transversal skills. The proposed learning environment encourages active participation in the activities and facilitates the retention of the concepts learned.
DPEP1 promotes drug resistance in colon cancer cells by forming a positive feedback loop with ASCL2
Authors: Zeng Cheng , Qi Guoping , Shen Ying , Li Wenjing , Zhu Qi , Yang Chunxia , Deng Jianzhong , Lu Wenbin , Liu Qian , Jin Jianhua 
Published: June 2022

AbstractBackgroundDrug resistance is an important factor affecting the efficacy of chemotherapy in patients with colon cancer. However, clinical markers for diagnosing drug resistance of tumor cells are not only a few in number, but also low in specificity, and the mechanism of action of tumor cell drug resistance remains unclear.MethodsDipeptidase 1 (DPEP1) expression was analyzed using the cancer genome atlas (TCGA) and genotype‐Tissue Expression pan‐cancer data. Survival analysis was performed using the survival package in R software to assess the prognostic value of DPEP1 expression in colon cancer. Correlation and Venn analyses were adopted to identify key genes. Immunohistochemistry, western blot, qRT–PCR, Co‐immunoprecipitation, and dual‐luciferase reporter experiments were carried out to explore the underlying associations between DPEP1 and Achaete scute‐like 2 (ASCL2). MTT assays were used to evaluate the role of DPEP1 and ASCL2 in colon cancer drug resistance.ResultsDPEP1 was highly expressed in colon cancer tissues. DPEP1 expression correlated negatively with disease‐specific survival but not with overall survival. Bioinformatics analysis and experiments showed that the expressions of DPEP1 and ASCL2 in colon cancer tissues were markedly positively correlated. Mechanistic research indicated that DPEP1 enhanced the stability of protein ASCL2 by inhibiting its ubiquitination‐mediated degradation. In turn, ASCL2 functioned as a transcription factor to activate the transcriptional activity of the DPEP1 gene and boost its expression. Furthermore, DPEP1 also could enhance the expression of colon cancer stem cell markers (LGR5, CD133, and CD44), which strengthened the tolerance of colon cancer cells to chemotherapy drugs.ConclusionsOur findings reveal that the DPEP1 enhances the stemness of tumor cells by forming a positive feedback loop with ASCL2 to improve resistance to chemotherapy drugs.
Anti‐inflammatory and immune‐mediated therapy for a case of febrile infection‐related epilepsy syndrome with rapid recurrence
Authors: Kurimoto Tomonori , Matsuoka Tsuyoshi , Ami Yuki , Kanno Koji , Fujii Takashi , Fujiwara Naoki , Matsuoka Takashi 
Published: June 2022

AbstractFebrile infection‐related epilepsy syndrome (FIRES) is a disease of unknown etiology, characterized by refractory frequent focal seizures, which require prolonged intensive care. We successfully treated a boy with FIRES with anti‐inflammatory and immunosuppressive therapy. This case suggests that an autoimmune mechanism may play a role in the development of FIRES.
Chiral Gold Clusters with Crosslinking Ligands: Geometric Structures and Chiroptical Activities
Authors: Shichibu Yukatsu , Konishi Katsuaki 
Published: June 2022

AbstractThe geometric diversity of ligand‐protected metal clusters has greatly provoked the interest in the chirality of gold clusters bearing crosslinking ligands, which are a subject of active investigations. This review introduces recent geometric and computational studies on chiral gold clusters whose metal core is covered by intramolecular crosslinking moieties. Following an overview of the structural constructions, the geometric features of the ligand shells and metal cores of chiral gold clusters are elucidated, where an emphasis is placed on the importance of helical patterns of the crosslinking ligands arranged around the gold cores. In addition, insights into the atomic‐level origin of chiroptical activity, which cannot be accessed solely through experiments, are presented from computational studies using numerical and theoretical approaches, thereby providing a novel methodology based on transition‐moment and transition‐density analyses.
Population Pharmacokinetics of Tralokinumab in Adult Subjects With Moderate to Severe Atopic Dermatitis
Authors: Soehoel Anders , Larsen Malte Selch , Timmermann Stine 
Published: June 2022

AbstractTralokinumab is the first biologic therapy for moderate‐to‐severe atopic dermatitis (AD) that specifically neutralizes interleukin‐13 activity, a key driver of AD signs and symptoms. Tralokinumab is a human immunoglobulin G4 monoclonal antibody administered subcutaneously every 2 weeks (with possibility of maintenance dosing every 4 weeks). This population pharmacokinetic (PK) analysis aimed to identify sources of PK variability and relevant predictors of tralokinumab exposure in adults with moderate to severe AD. Nonlinear mixed‐effect modeling, including covariate analysis, was used on a data set including 2561 subjects (AD, asthma, healthy) from 10 clinical trials. A 2‐compartment model with first‐order absorption and elimination adequately described the tralokinumab PK. Body weight was identified as a relevant predictor of tralokinumab exposure; other covariates including age, sex, race, ethnicity, disease type, AD severity, and renal and hepatic impairment were not. For body weight, the difference in exposure between the upper‐ and lower‐weight quartiles in patients with AD was <2‐fold, supporting the appropriateness of flat dosing (300 mg). Given the reduced exposure associated with higher body weight, coupled with the reduced exposure provided by dosing every 4 weeks, it is uncertain whether higher‐weight patients will achieve sufficient exposure to maintain efficacy if dosed every 4 weeks instead of the standard every 2 weeks.
Detection of resistance loci to English grain aphid in common wheat
Authors: Zhankui Zeng , Zhipeng Han , Chunping Wang , Zhenghong Wang , Cheng Guo , Liming Wang , Li Gao 
Published: June 2022

AbstractThe English grain aphid (Sitobion avenae F.) causes significant yield and quality losses in wheat (Triticum aestivum L.) worldwide, and the use of insect‐resistant wheat cultivars reduces the loss level. The present study aimed to identify quantitative trait loci (QTL) and perform putative candidate gene evolutionary analysis for English grain aphid resistance in 261 F2:5 recombinant inbred lines (RILs) derived from the cross Amigo/XN1376. The RIL population and parents were genotyped with the Wheat 55K single nucleotide polymorphism (SNP) array. A total of 14,694 polymorphic SNPs were used to construct a genetic linkage map. One major effect of QTL was detected in F2:5 RILs and was located on chromosome 3DL, explained 19.33% phenotypic variance, temporarily designated as QSa3.haust‐3D. It was located in a 2.73 Mb, flanked by markers AX‐109429351 and AX‐110834100, from 550.25 to 552.98 Mb according to Chinese Spring reference genome. This region contained 39 annotated genes. Eight putative candidate genes for resistance to English grain aphid were identified among these genes. The QTL with closely linked SNP markers could be useful for marker‐assisted selection for resistance to English grain aphid for wheat breeding.
How and when does perceived greenwashing affect employees' job performance? Evidence from China
Authors: Li Wei , Li Weining , Seppänen Veikko , Koivumäki Timo 
Published: June 2022

AbstractDespite increasing interest in the implications of greenwashing, few studies have examined the underlying mechanism and contingency of how greenwashing affects employee outcomes. In this study, we develop a mediated moderation model to analyze the impact of perceived greenwashing on employee job performance (i.e., task performance and organizational citizenship behavior). Using a questionnaire survey of 400 employees in 20 Chinese companies, the results reveal that perceived greenwashing negatively affects job performance and that the relationship is mediated by organizational cynicism. Furthermore, employees' green values strengthen the indirect negative relationship between perceived greenwashing practices and job performance through organizational cynicism. The study contributes to addressing the long‐discussed problem of whether greenwashing pays vis‐à‐vis a human resource management perspective and micro‐level approach. The findings indicate that a close cooperation between the human resources and corporate environmental responsibility management departments is required to achieve the sustainable development of businesses.
Higher BCG‐induced trained immunity prevalence predicts protection from COVID‐19: Implications for ongoing BCG trials
Authors: Singh Samer , Kishore Dhiraj , Singh Rakesh K. , Pathak Chandramani , Ranjan Kishu 
Published: June 2022

AbstractEndeavors to identify potentially protective variables for COVID‐19 impact on certain populations have remained a priority. Multiple attempts have been made to attribute the reduced COVID‐19 impact on populations to their Bacillus–Calmette–Guérin (BCG) vaccination coverage ignoring the fact that the effect of childhood BCG vaccination wanes within 5 years while most of the COVID‐19 cases and deaths have occurred in aged with comorbidities. Since the supposed protection being investigated could come from heterologous ‘trained immunity’ (TI) conferred by exposure to Mycobacterium spp. (i.e., environmental and BCG), it is argued that the estimates of the prevalence of TI in populations currently available as latent tuberculosis infection (LTBI) prevalence would be a better variable to evaluate such assertions. Indeed, when we analyze the European populations (24), and erstwhile East and West Germany populations completely disregarding their BCG vaccination coverage, the populations with higher TI prevalence consistently display reduced COVID‐19 impact as compared to their lower TI prevalence neighbors. The TI estimates of the populations not the BCG coverage per se, negatively correlated with pandemic phase‐matched COVID‐19 incidences (r(24): −0.79 to −0.57; p‐value < .004), mortality (r(24): −0.63 to −0.45; p‐value < .03), and interim case fatality rates (i‐CFR) data. To decisively arrive at dependable conclusions about the potential protective benefit gained from BCG vaccination in COVID‐19, the ongoing or planned randomized controlled trials should consciously consider including measures of TI as: (a) all individuals immunized do not respond equally, (b) small study groups from higher background TI could fail to indicate any protective effect.