bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Alcohol‐Associated Hepatitis Is Associated With Specific Changes in Gut‐Modified Bile Acids
Authors: Muthiah Mark D. , Smirnova Ekaterina , Puri Puneet , Chalasani Naga , Shah Vijay H. , Kiani Calvin , Taylor Stephanie , Mirshahi Faridoddin , Sanyal Arun J. 
Published: January 2022

The perturbations in bile acids (BAs) in alcohol‐associated hepatitis (AH) and its relationship to disease severity is not well defined. The aims of this study were to define (1) the effects of heavy alcohol consumption on BAs and related microbiome, (2) the additional changes with AH, and (3) the relationship of these changes to disease severity. In this multicenter study, plasma and fecal BAs and related microbiome were interrogated in healthy individuals, heavy drinking controls (HDCs) without overt liver disease, and AH. Compared to healthy controls, HDCs had increased glycine‐conjugated 7α and 27α primary BAs and increased secondary BA glycocholenic sulfate (multiple‐comparison adjusted P < 0.05 for all). Plasma‐conjugated cholic and chenodeoxycholic acid increased in AH along with the secondary BAs ursodeoxycholic and lithocholic acid (P < 0.001 for all), whereas deoxycholic acid decreased; however fecal concentrations of both deoxycholic acid and lithocholic acid were decreased. Glycocholenic acid further increased significantly from HDCs to AH. HDCs and AH had distinct plasma and fecal BA profiles (area under the curve, 0.99 and 0.93, respectively). Plasma taurochenodeoxycholic acid and tauroursodeoxycholic acid were directly related to disease severity, whereas fecal ursodeoxycholic acid was inversely related. The fecal abundance of multiple taxa involved in formation of secondary BAs, especially deoxycholic acid (Clostridium cluster XIVa) was decreased in AH. Multiple genera containing taxa expressing 3α, 3β, 7α, and 7β epimerases were decreased with concordant changes in fecal BAs that required these functions for formation. Conclusion: There are distinct changes in BA‐transforming microbiota and corresponding BAs in AH that are related to disease severity.
The evolution and inter‐sectoral interaction mechanism of China's national medical alliance: An analysis based on complex systems theory
Authors: Feng Chong , Zhu Jianping , Chen Yusheng 
Published: January 2022

AbstractThis work investigates the performance and inter‐sectoral interaction mechanism of China's largest vertically integrated care network, the national medical alliance (NMA). The data collected derive from the China Health Statistics Bulletin and the China Health Statistical Yearbook for the period 2009–2018. The data include 64 observation indicators for five medical sectors in the NMA, namely, tertiary hospitals (THS), secondary hospitals (SHS), community health centres (CHCS), township hospitals (TsHS) and professional public health institutions (PPHIS). This research combines complex systems theory with a multilevel structural dynamic factor model, and yields two main results. First, although the trend for the NMA's global factor is increasing, the evolutionary paths for sectoral factors differ substantially. Among the sectoral factors, the sectoral factor of THS continued to decline, and neither the sectoral factor of CHCS nor the sectoral factor of TsHS has significantly improved. Then, the interaction mechanism between the various NMA sectors is investigated. While a close relationship has been formed between THS and CHCS and between SHS and CHCS, there remains no close two‐way relationship between either THS and TsHS or THS and SHS. Thus, going forward, to reach the policy expectations, China's NMA implementation must consider the interaction between different constituent sectors.
Why most psychological research findings are not even wrong
Authors: Scheel Anne M. 
Published: January 2022

AbstractPsychology's replication crisis is typically conceptualized as the insight that the published literature contains a worrying amount of unreplicable, false‐positive findings. At the same time, meta‐scientific attempts to assess the crisis in more detail have reported substantial difficulties in identifying unambiguous definitions of the scientific claims in published articles and determining how they are connected to the presented evidence. I argue that most claims in the literature are so critically underspecified that attempts to empirically evaluate them are doomed to failure—they are not even wrong. Meta‐scientists should beware of the flawed assumption that the psychological literature is a collection of well‐defined claims. To move beyond the crisis, psychologists must reconsider and rebuild the conceptual basis of their hypotheses before trying to test them.
Hesperidin delays cell cycle progression into the G0/G1 phase via suspension of MAPK signaling pathway in intrahepatic cholangiocarcinoma
Authors: Deng Jie , Liu Li , Li Li , Sun Jianhai , Yan Fei 
Published: January 2022

AbstractIntrahepatic cholangiocarcinoma (iCCA) derived from epithelial cells of bile ducts is highly aggressive tumor. Hesperidin extracted from citrus fruits is a promising antitumor compound. The purpose of this study is to explore molecular mechanism by which hesperidin affects cholangiocarcinoma progression. Cellular functional experiments were performed and subcutaneous transplant xenograft model was established. Our findings indicated that hesperidin suppressed iCCA cell proliferation in time‐ and concentration‐dependent manners. Hesperidin treatment induced cell cycle arrest at G0/G1 phase, whereas it has no effect on cell apoptosis. Further, data revealed that hesperidin attenuated MEK5 and ERK5 phosphorylation and inhibited ERK5 nuclear localization by reducing MEKK2 activity in MAPK signaling pathway. It could cause alterations in expression of the downstream genes, including CDK4, CDK6 (cell cycle protein kinases), Cyclin D1 (a G1/S checkpoint), P21, and P27 (two G1‐checkpoint CDK inhibitors), thereby arresting cell cycle distribution of iCCA cells in the G0/G1 phase. BIX02189 treatment, a specific inhibitor of MEK5, in combination with hesperidin displayed synergistic inhibitory effects on cell cycle arrest and gene expressions. Furthermore, hesperidin administration alone or in combination with MEK5 inhibitor BIX02189 restrained iCCA tumor growth in vivo. Taken together, these results confirmed that hesperidin regulated the expression of cell cycle‐related genes by inhibiting the activation of MEKK2/MEK5/ERK5 signaling pathway, inducing iCCA cell cycle arrest at the G0/G1 phase. Our study provides a theoretical foundation and experimental basis for further development of hesperidin as a therapeutic agent for iCCA treatment.
Thinking under fire: Mentalization‐based couple therapy for high conflict and domestically abusive couples'
Authors: McCann Damian 
Published: January 2022

AbstractBackgroundThe question of working psychotherapeutically with high conflict and domestically abusive couples is one that continues to raise anxieties within the field.AimsEmbracing a relational approach offers an alternative perspective to the more familiar individual‐based treatment interventions.Materials and MethodsDrawing on my therapy with a married couple who presented in a crisis following an abusive incident, I will outline how I approached the assessment, my understanding of their presenting problem and the basis on which the therapy was conducted.ResultsDuring the eight months of treatment, the couple had frequent breakdowns in communication, and I found myself drawing on a range of mentalization‐based techniques that supported and maintained the working alliance.DiscussionHelping the couple break the damaging effects of the abuse, which was seriously affecting them and impacting their children, provides a particular focus for this submission.ConclusionThe rationale for undertaking couple therapy in situations of domestic violence and abuse was examined. The dynamic elements of theory underpinning practice, together with mentalization‐based techniques designed to tackle disregulated states of mind that may lead to violent and abusive exchanges, were considered through the presentation of a case example.
Disseminating online parenting resources in the community during the COVID‐19 pandemic: Lessons learned
Authors: Lasecke Meagan , Baeza‐Hernandez Katerina , Dosovitsky Gilly , DeBellis Amanda , Bettencourt Brianna , Park Alayna L. , Bunge Eduardo L. 
Published: January 2022

AbstractOnline parenting programs are an effective way to teach behavioral management skills to parents in the absence of in‐person resources. This community‐engaged study aimed to examine strategies for disseminating online parenting resources in schools. Online resources were disseminated to parents in a Northern California school district. Dissemination strategies were informed by conversations with school principals, teachers, and parents and considered agent, message, and format. A total of 685 parents and teachers clicked on the online resources: 151 parents and 114 teachers attended synchronous classes. The use of dissemination strategies had a compounding influence on the number of synchronous class attendees and clicks. Emails sent by the school district yielded the greatest number of clicks, which was influenced by message content and format. A community–academic partnership (CAP) led to the dissemination of evidence‐based online parenting resources to a large population and led to lessons learned that could inform future research involving CAPs.
Intramuscular and intratendinous placenta‐derived mesenchymal stromal‐like cell treatment of a chronic quadriceps tendon rupture
Authors: Maleitzke Tazio , Reinke Petra , Agres Alison N. , Alves Sónia A. , Akyüz Levent , Fleckenstein Florian N. , Bichmann Anna , Ofir Racheli , Perka Carsten , Duda Georg N. , Winkler Tobias 
Published: January 2022

AbstractBackgroundQuadriceps tendon ruptures (QTRs) are rare but debilitating injuries, often associated with chronic metabolic conditions or long‐term steroid treatment. While the surgical treatment for acute QTRs is described thoroughly, no common strategy exists for the often frustrating treatment of chronic, reoccurring QTRs. The pro‐angiogenic and immunomodulatory properties of placenta‐derived adherent mesenchymal stromal‐like (PLX‐PAD) cells have been described to protect musculoskeletal tissues from inflammation and catabolic cytokine migration, yet little is known about the regenerative potential of PLX‐PAD cells in repetitively damaged tendon tissue.CaseWe report the case of an 80‐year‐old male patient with a chronic three‐time QTR of his right knee. The quadriceps tendon was reconstructed applying a conventional suture anchor repair procedure combined with a synthetic mesh augmentation and additional intramuscular and intratendineous PLX‐PAD cell injections as an individualized treatment approach. No adverse events were reported, and excellent radiological and functional outcomes with a passive range of motion of 0/0/120° knee extension‐flexion were observed at the 12 month follow‐up. Gait analysis confirmed restoration of joint motion, including gait speed, deficit in step length, and knee extensor muscle strength (pre‐surgery: 0.98 m/s, 40 cm, 42.4 ± 12.4 N; 9 months post‐surgery: 1.07 m/s, 0 cm, 10.4 ± 18.9 N) as well as hyperextension throughout stance and late swing phases (pre‐surgery: −11.2 ± 0.9°; 9 months post‐surgery: −2.7 ± 1.6°). Postoperative lymphocyte and cytokine analyses from the patient's peripheral blood serum suggested a systemic short‐term immunoregulatory reaction with postoperatively increased interleukin (IL)‐6 (pre‐surgery: 0.79 pg/mL; day 1: 139.97 pg/mL; day 5: 5.58 pg/mL; 9 months: 1.76 pg/mL) and IL‐10 (pre‐surgery: 0.9 pg/mL; day 1: 1.21 pg/ mL; day 5: 0.3 pg/mL; 9 months: 0.34 pg/mL) levels that decreased again over time.ConclusionsHerein, we demonstrate a successfully treated chronic QTR with a synergistic surgical and biological reconstructive treatment approach. This local add‐on treatment with PLX‐PAD cells may be considered in specific cases of chronic QTRs, not susceptible to traditional suture anchor procedures and which exhibit a high risk of treatment failure. Further scientific engagement is warranted to explore underlying immunomodulatory mechanisms of action behind PLX‐PAD cell treatment for tendon injuries.
Comparison of photon transport efficiency in simple scintillation electron detector configurations for scanning electron microscope
Authors: Schauer Petr 
Published: January 2022

AbstractThe purpose of this paper is to find some general rules for the design of robust scintillation electron detectors for a scanning electron microscope (SEM) that possesses an efficient light‐guiding (LG) system. The paper offers some general instructions on how to avoid the improper design of highly inefficient LG configurations of the detectors. Attention was paid to the relevant optical properties of the scintillator, light guide, and other components used in the LG part of the scintillation detector. Utilizing the optical properties of the detector components, 3D Monte Carlo (MC) simulations of photon transport efficiency in the simple scintillation detector configurations were performed using the computer application called SCIUNI to assess shapes and dimensions of the LG part of the detector. The results of the simulation of both base‐guided signal (BGS) configurations for SE detection and edge‐guided signal (EGS) configurations for BSE detection are presented. It is demonstrated that the BGS configuration with a matted disc scintillator exit side connected to the cylindrical light guide without optical cement is almost always a sufficiently efficient system with a mean LG efficiency of about 20%. It is simulated that poorly designed EGS strip configurations have an extremely low mean LG efficiency of only 0.01%, which can significantly reduce detector performance. On the other hand, no simple nonoptimized EGS configuration with a light guide widening to a circular or square profile, with a polished cemented scintillator and with an indispensable hole in it has a mean LG efficiency lower than 6.5%.
Sensitive electrochemical recognition ofα‐synucleinprotein in human plasma samples using bioconjugated gold nanoparticles: An innovative immuno‐platform to assist in the early stage identification of Parkinson's disease by biosensor technology
Authors: Aminabad Esmaeil Darvish , Hasanzadeh Mohammad , Ahmadalipour Ali , Mahmoudi Tohid , Feizi Mohammad Ali Hosseinpour , Safaralizadeh Reza , Mobed Ahmad 
Published: January 2022

AbstractThis research work explains the development of an electrochemical immunosensor for the selective recognition of SNCA in human biofluids. An innovative protocol was proposed for the green synthesis of gold nanoparticle‐supported dimethylglyoxime (AuNPs@DMGO) using one‐step electrogeneration method. Also, the application of AuNPs@DMGO for the sensitive quantification ofα‐Synuclein (SNCA) protein and its biomedical analysis. So, an innovative sandwich immunosensor was designed for the sensitive identification of SNCA antigen in an aqueous solution. The gold nanoparticles (AuNPs) were decorated on the surface of the glassy carbon electrode by chronoamperometry technique to provide appropriate immobilization surface with a large number of active sites for immobilization of specific biotinylated antibody (Ab1) and against SNCA protein. Then, the sandwich‐type immuno‐platform was completed by the attachment of secondary antibody (HRP conjugated Ab [Ab2]) to the primary complexes on the surface of the electrode. For the first time, α‐Synuclein protein was measured with an acceptable linear range of 4–64 ng/mL and a lower limit of quantification of 4 ng/mL. Benefiting from the simplicity and high sensitivity, the proposed method shows a potential of employment in clinical applications and high‐throughput screening of Parkinson's disease using POC.
Characterization of a CH3NH3PbI3 perovskite microwire by Raman spectroscopy
Authors: Chen Xiaoming , Xu Ying , Wang Zixian , Wu Ren‐Jie , Cheng Horng‐Long , Chui Hsiang‐Chen 
Published: January 2022

AbstractWe investigated the structural properties of organic–inorganic lead halide perovskite microwires through joint experimental and theoretical Raman spectroscopy. High‐quality methyl ammonium lead iodide (MAPBI3, MA = CH3NH3+) microwires with tetragonal phases and explicit crystalline orientation were prepared. Raman spectroscopy was used in studying the as‐prepared microwires and determining not only the crystal quality but also crystalline orientation of MAPBI3 with the aid of normal mode calculations. High‐quality structural features were verified by well‐resolved Raman peaks related to the inorganic cage (IPbI stretching and bending modes below 100 cm−1), organic MA cation motions (libration ~150–200 cm−1 and torsional ~200–250 cm−1), a coupled mode of cage and MA cation liberation (~105–110 cm−1), and the almost undetectable degraded signal at 95 cm−1. In micro‐Raman mapping, an intensity ratio of 105 and 95 cm−1 was used for the direct imaging of crystal quality along the microwire. The characterization of the crystalline orientation of MAPBI3 microwires posed a challenge, which can be addressed by using polarized Raman technique with normal mode analysis. We believe that the proposed method can be extended to other organic–inorganic lead halide perovskites with different structures, such as polycrystalline films and single crystals.
Capillary electrophoresis applied for the determination of acidity constants and limiting electrophoretic mobilities of ionizable herbicides including glyphosate and its metabolites and for their simultaneous separation
Authors: Graf Hannes Georg , Biebl Sonja Maria , Müller Linda , Breitenstein Christina , Huhn Carolin 
Published: January 2022

AbstractThermodynamic acidity constants and limiting ionic mobilities were determined for polyprotic non‐chromophore analytes using capillary electrophoresis with capacitively coupled contactless conductivity detection. It was not necessary to work with buffers of identical ionic strength as ionic strength effects on effective electrophoretic mobilities were corrected by modeling during data evaluation (software AnglerFish). The mobility data from capillary electrophoresis coupled to conductivity detection were determined in the pH range from 1.25 to 12.02 with a high resolution (36 pH steps). With this strategy, thermodynamic acidity constants and limiting ionic mobilities for various acidic herbicides were determined, sometimes for the first time. The model analytes included glyphosate, its metabolites, and its acetylated derivates (aminomethyl phosphonic acid, glyoxylic acid, sarcosine, glycine, N‐acetyl glyphosate, N‐acetyl aminomethyl phosphonic acid, hydroxymethyl phosphonic acid). The obtained data were used in simulations to optimize separations by capillary electrophoresis. Simulations correlated very well to experimental results. With the new method, the separation of glyphosate from interfering components like phosphate in beer samples was possible.
Structural and functional landscape connectivity for lesser prairie‐chickens in the Sand Shinnery Oak Prairie Ecoregion
Authors: Schilder Lucas J. , Heintzman Lucas J. , McIntyre Nancy E. , Harryman Samuel , Hagen Christian A. , Martin Russell , Boal Clint W. , Grisham Blake A. 
Published: January 2022

AbstractThe lesser prairie‐chicken (Tympanuchus pallidicinctus) is a species of conservation concern on the Southern High Plains of Texas and New Mexico, USA. Because fragmentation and isolation have increased since pre‐settlement, dispersal through this heterogeneous landscape may be constrained, with serious implications for conservation and management of this species. Our objectives were to quantify landscape connectivity for lesser prairie‐chickens within a patch network of potentially isolated leks (breeding display grounds), and examine effects of land use change on modeled lesser prairie‐chicken movements through the landscape. We used graph theory to quantify structural landscape connectivity and circuit theory to quantify functional landscape connectivity for lesser prairie‐chickens in the Sand Shinnery Oak Prairie Ecoregion of the Southern High Plains. There was a high degree of clustering among leks (n = 1,023 leks), with a 41.9‐km coalescence distance of the network. We identified 3 leks as cutpoints within the network, meaning if the habitat patches containing these leks were fragmented, the remaining leks would become isolated from each other. We also identified several leks that were important for maintaining overall population connectivity for lesser prairie‐chickens on the Southern High Plains. Conservation Reserve Program land was important for maintaining connectivity among leks to the north and west of the main lek core area located in New Mexico, but wind energy development constrained pathways to the north and south of this main lek core area. Our results suggest that landscape connectivity was reduced by row‐crop agriculture and energy production and facilitated by the Conservation Reserve Program within the Sand Shinnery Oak Prairie Ecoregion.
Cyclic oxidation behavior of MCrAlX coatings made by electrolytic codeposition
Authors: Zhang Ying , Witman Jason , Dryepondt Sebastien 
Published: January 2022

AbstractMCrAlY coatings made by electron beam physical vapor deposition or thermal spray are often used to provide protection for superalloys against high‐temperature oxidation and corrosion. In addition to Y, other elements such as Ta, Hf, and Si have been reported to be beneficial. In this paper, NiCoCrAlX (where X = Y, Ta, Hf, and/or Si) coatings were fabricated via an electrolytic codeposition process in a sulfate‐based Ni–Co plating solution. As compared to uncoated René 80 substrates, the electro‐codeposited NiCoCrAlX coatings demonstrated superior performance during oxidation testing with a 1‐h cycle frequency at 1000°C and 1100°C. Further improved oxidation resistance was observed for the coatings with Hf and Si additions, but not with the addition of Ta. The levels of S, Y, and O in the electrodeposited NiCoCrAlY coating were measured by high‐resolution glow discharge mass spectrometry and were compared to those in thermal spray coatings.
Robotic deep inferior epigastric perforator flap harvest in breast reconstruction
Authors: Daar David A. , Anzai Lavinia M. , Vranis Neil M. , Schulster Michael L. , Frey Jordan D. , Jun Min , Zhao Lee C. , Levine Jamie P. 
Published: January 2022

AbstractIntroductionReducing donor site morbidity after deep inferior epigastric artery perforator (DIEP) flap harvest relies mainly upon maintaining integrity of the anterior rectus sheath fascia. The purpose of this study is to describe our minimally‐invasive technique for robotic DIEP flap harvest.MethodsA retrospective review of four patients undergoing seven robotic‐assisted DIEP flaps from 2019 to 2020 was conducted. Average patient age and BMI were 52 years (range: 45–60 years) and 26.7 kg/m2 (range: 20.6–32.4 kg/m2), respectively. Average follow‐up was 6.31 months (range: 5.73–7.27 months). Robotic flap harvest was performed with intramuscular perforator dissection in standard fashion, followed by the transabdominal preperitoneal (TAPP) approach to DIEP pedicle harvest using the da Vinci Xi robot. Data was collected on demographic information, perioperative characteristics. Primary outcomes included successful flap harvest as well as donor site morbidity (e.g., abdominal bulge, hernia, bowel obstruction, etc.).ResultsAll four patients underwent bilateral abdominally‐based free flap reconstruction. Three patients received bilateral robotic DIEP flaps, and one patient underwent unilateral robotic DIEP flap reconstruction. The da Vinci Xi robot was used in all cases. Average flap weight and pedicle length were 522 g (range: 110–809 g) and 11.2 cm (range: 10–12 cm), respectively. There were no flap failures, and no patient experienced abdominal wall donor site morbidity on physical exam.ConclusionWhile further studies are needed to validate its use, this report represents the largest series of robotic DIEP flap harvests to date and is a valuable addition to the literature.