bluuebunny/crossref_metadata_2025_split huggingface dataset:
Appearance, microstructure and bioactive components of Bletilla striata tuber as affected by different drying methods
Authors: Zhang Man , Li Lihong , Han Tingting , Wang Jie , Fang Hailing , Lin Ding 
Published: March 2022

Abstract
Bletilla striata tuber is widely used as healthy food and herbal medicine for its hemostasis, anti-ulcer, promoting wound healing, antibacterial and anti-inflammatory and immune regulation functions. Drying method will affect the qualities of B. striata tuber including appearance and more importantly the content of bioactive components, which play decisive roles of the medical and tonic functions. To investigate the effects of drying method on the qualities of B. striata tuber, samples were treated with five drying methods containing hot-air drying (HD), infrared drying (ID), microwave drying (MD), vacuum drying (VD), and freeze drying (FD) in this study. After drying, samples were evaluated for their appearances, microstructures and the contents of bioactive components. Results showed that FD-dried samples kept most of the appearances and microstructures of fresh B. striata tuber. Samples treated with other methods were deformed and discolored to various degree. The contents of bioactive components containing B. striata polysaccharides (BSP), phenols and militarine were disproportionately influenced by different drying methods, which had been discussed in detail in the article. The research results will provide useful suggestion for production, development and further research of B. striata.
Is There any Role for Autophagy in Progression of Liver Fibrosis in Chronic Hepatitis B Patients on Antiviral Treatment?
Authors: Lankarani Kamran B. , Sadidoost Atefeh , Fattahi Mohammadreza , Fard Saeid Amirzadeh , Mokarram Pooneh 
Published: March 2022

Abstract
IntroductionDespite antiviral treatment, there are patients with chronic hepatitis B (CHB) who progress to cirrhosis. Enhancement of autophagy has been implicated in the proliferation of hepatitis B in hepatocytes. This study was designed to evaluate the possible role of autophagy in the progression of liver fibrosis in patients on antiviral therapies and fully suppressed viral replication .MethodsPatients on anti-hepatitis B nucleotide treatments for at least two years and who were not cirrhotic at baseline but later developed cirrhosis were identified. For the control group, patients who were on the nucleotide regimens and did not have cirrhosis at baseline and on follow-up were randomly selected from our registry. Serum Beclin-1 and LC3 measured by enzyme-linked immunosorbent tests were compared between two groups.ResultsBeclin-1 level was higher in those with cirrhosis, but no statistical difference was seen for the level of LC3.ConclusionAutophagy may have a role in progression to cirrhosis in patients with CHB. Future prospective larger studies are required to find the effect of blockage of autophagy on liver disease progression in this group.
Diabetic microenvironment preconditioning of adipose tissue-derived mesenchymal stem cells enhances their anti-diabetic, anti-long-term complications, and anti-inflammatory effects in type 2 diabetic rats
Authors: Su Wanlu , YU Songyan , Yin Yaqi , Li Bing , Xue Jing , Wang Jie , Gu Yulin , Zhang Haixia , Lyu Zhaohui , MU Yiming , Cheng Yu 
Published: March 2022

Abstract
Background
Mesenchymal stem cells (MSCs) exert anti-diabetic effects and improve long-term complications via secretory effects that regulate macrophage polarisation and attenuate inflammation. Enhancing the efficacy of MSCs needs to be explored further. The in vitro culture microenvironment influences the secretory profile of MSCs. Therefore, we hypothesised that a diabetic microenvironment would promote the secretion of cytokines responsible for macrophage polarisation, further attenuating systemic inflammation and enhancing the effects of MSCs on type 2 diabetes (T2D) and long-term diabetic complications.
Methods
Preconditioned adipose-derived mesenchymal stem cells (pre-ADSCs) were obtained after co-cultivating ADSCs in a diabetic metabolic environment (including high sugar, advanced glycation end-product, and lipopolysaccharides). The regulatory effects of pre-ADSCs on macrophages were observed in vitro. A T2D rat model was induced with a high-fat diet for 32 weeks combined with an intraperitoneal injection of streptozotocin. Sprague-Dawley (SD) rats were divided into four groups: normal group, diabetes without treatment group (PBS), ADSC treatment group, and pre-ADSC treatment group. ADSCs and pre-ADSCs were intravenously administered weekly to SD rats for 6 months, and then glucose homeostasis and long-term diabetic complications were evaluated in each group.
Results
The secretion of cytokines related to M2 macrophage polarisation (IL-6, MCP-1, etc.) was increased in the pre-ADSC group in the in vitro model. Pre-ADSC treatment significantly maintained blood glucose homeostasis, reduced insulin resistance, promoted islet regeneration, and ameliorated the complications related to diabetes in rats (chronic kidney disease, non-alcoholic steatohepatitis, lung fibrosis, and cataract) compared to the ADSC group (P < 0.05). Additionally, the number of anti-inflammatory M2 macrophage phenotypes was enhanced in tissues following pre-ADSC injections. Moreover, the expression of pro-inflammatory genes (iNOS, TNF-α, IL-1β) was reduced whereas that of anti-inflammatory genes (Arg1, CD206, and Il-10) was increased after cultivation with pre-ADSCs.
Conclusion
Diabetic microenvironment-preconditioned ADSCs effectively strengthen the capacity against inflammation and modulate the progress of long-term T2D complications.
Ulcerative Colitis And Periodontitis – A Cross Sectional Study From A Norwegian Cohort Diagnosed With Ulcerative Colitis For More Than 10 Years
Authors: Haugbo Helena. O. , Klepp Pasquale , Verket Anders 
Published: March 2022

Abstract
Background: Inflammatory bowel disease (IBD) includes the major diseases Crohn’s disease (CD) and ulcerative colitis (UC). IBD is understood to be an inflammatory systemic disorder that may have an impact on periodontal disease through shared inflammatory mechanisms. The two subtypes of IBD are often described together although they differ with regards to affected sites in the gastrointestinal tract, histology and oral manifestations. The primary aim of this study was to investigate the prevalence of periodontitis in a cohort of UC according to the classification scheme in periodontology. The secondary aim was to assess a potential correlation of periodontal status with UC disease parameters, years with UC diagnosis and oral health related quality of life. Method: In a cross-sectional study a cohort from a community hospital in Norway with confirmed ulcerative colitis diagnosis for more than 10 years was comprehensively diagnosed according to the new classification of periodontitis. Periodontal parameters, oral health related quality of life (OHRQoL) (OHIP-14), demographic data were collected. Previous UC data including colon activity index (CAI), Mayo score and years of UC diagnosis was used to correlate with the periodontal status.Results: A total of 50 of 63 invited patients participated. According to the new classification scheme on periodontitis, 74% of the patients presented periodontitis. No correlation was found between periodontitis (stage, grade, BoP or PPD>6mm) and CAI, Mayo score, or years with UC diagnosis. The OHIP-14 scores reported were in line with that of a normal population.Conclusions: Localized stage III periodontitis grade B was the most prevalent periodontal diagnosis. No correlation between periodontal disease and previous UC disease markers or years with UC diagnosis was found. In this population of subjects with well-treated UC, both the prevalence of periodontitis and OHRQoL were in line with that of the general population.
Compound and Simple Leaf Woody Species of The Chilean Matorral Are Equally Affected by Extreme Drought
Authors: Ortíz José , Hernández-Fuentes Carolina , Sáez Patricia L. , Saz Néstor Fernández del , Cavieres Lohengrin A. 
Published: March 2022

Abstract
Two main leaf types are recognized among vascular plant species: compound and simple. Compound leaves are believed to be photosynthetically more productive than simple ones, by diluting mass tissue in more projected area. Conversely, simple leaves are believed to be more stress tolerant by packing mass tissue in less projected area during stress like drought. Nevertheless, convective cooling is more efficient in compound than simple leaves, a process that could alleviate water loss in drought periods. In Central Chile, woody species with simple and compound leaves coexist. This zone is facing a mega-drought event, causing browning and tree mortality. However, photosynthetic limitations on both types of leaves in drought conditions have not been addressed so far. We measured photosynthetic limitations in well-watered and drought conditions in three compound and three simple leaf species, and drought response ratio were obtained. We hypothesized that with no water limitation compound leaf species will show higher net photosynthesis (AN) than simple leaf species associated with a higher mesophyll conductance (gm). Nevertheless, opposite results are expected for simple leaves because their stress-tolerant physiology, showing fewer changes in their photosynthetic traits. We found that (gm) and (AN) were larger in compound leaves in well-watered conditions. Under drought conditions, both types of leaves were negatively affected despite foliar temperature in compound leaf species was 4°C lower. Our result suggests that regardless leaf shape matorral species in Central Chile will be seriously affected in their AN due to the megadrought currently affecting this zone.
Amiodarone improves anemia in a murine model of sickle cell disease and is associated with increased erythrocyte bis(monoacylglycerol) phosphate
Authors: Venugopal Jessica , Wang Jintao , Guo Chiao , Eitzman Daniel T. 
Published: March 2022

Abstract
Sickle cell disease (SCD) is with associated altered plasma and erythrocyte lipid profiles. In a previous study, SCD mice with deficiency of proprotein convertase subtilisin/kexin type 9 (PCSK9) were observed to have more severe anemia and increased sickling compared to control SCD mice. Although PCSK9 affects circulating low density lipoprotein (LDL) by regulation of the LDL receptor, the effect of PCSK9 on anemia was independent of LDL receptor expression. In the current study, erythrocyte metabolomics were performed and revealed altered erythrocyte lipid species between SCD mice with and without PCSK9. Of particular interest, the late endosome-specific lipid bis(mono)acylglycerol phosphate (BMP) 44:12 was markedly decreased in erythrocytes from SCD mice deficient in PCSK9 mice relative to control SCD mice. Incubation of sickle erythrocytes with a neutralizing antibody to BMP increased erythrocyte sickling in vitro. In vitro treatment of SCD erythrocytes with amiodarone (1.5 uM) or medroxyprogesterone (6.75 uM), two pharmacologic compounds known to increase BMP, resulted in reduced erythrocyte sickling. Treatment of SCD mice with amiodarone (10 mg/kg) for 2 weeks resulted in increased BMP, improvement in anemia with reduced reticulocytosis, and decreased ex vivo sickling. In conclusion, severity of anemia in SCD is improved with amiodarone treatment, an effect which may be mediated through increased erythrocyte BMP.
WITHDRAWN: Permeability enhancement approaches of dexamethasone using niosomal gel for treating keloid
Authors: Sivaranjani  
Published: March 2022

Abstract
Background
Niosome are surfactant based vesicular drug delivery system that improve the residence time of drugs by overcoming the inherent anatomical barrier. Dexamethasone is used as an intralesional injection for treating keloids, which is formulated as niosomal gel for the inhibition of keloid fibroblast. This study aimed to formulate the Dexamethasone niosome suspension and the suspension was incorporated into the gel. Niosomal suspension of Dexamethasone was formulated by thin-film hydration method using Tween 20, Span 60, and Tween 80. Tween 80 niosome formulation was optimized using Design of expert software (DOE). Optimized niosome were characterized for particle size, zeta potential, Atomic Force Microscopy (AFM), entrapment efficiency, in vitro release, cell proliferation studies, and cell permeation studies. The optimized tween 80 formulation was incorporated into the carbopol gel base and evaluated.
Results
The desirable quantity of tween 80 was 86.2 µM and cholesterol was 38.9 µM. Spherical shaped vesicle with particle size of tween 80 suspension was 498.1 ± 1.1710 nm, entrapment efficiency was 85.2%±2.851, and in vitro release was 96.5%±2.88 at the end of 6 hr. Cell proliferation studies were performed to confirm the anti-keloid activities using MTT assay which decreased the cell viability in the range between 49–58%. Cell permeability studies were performed using Caco-2 cells showed a low Transepithelial electrical resistance (TEER) value after 2 hr treatment indicating more permeation than the drug solution.
Conclusion
The results confirm niosomal gel with Tween 80 enhances the skin permeation of Dexamethasone.
Integrated Analysis of Serum Lymphocytes Develops a Nomogram for Predicting Prognosis of Intracranial Germ Cell Tumors to Chemo-Radiotherapy
Authors: Wang Hairong , Huang He , Mou Yonggao , Chi Peidong , Lin Xiaopin , Chen Hongyu , Chen Jiangen , Chen Zhongping , Yang Qunying , Guo Chengcheng 
Published: March 2022

Abstract
Introduction:
It is common to evaluate lymphocytes subsets in virus infection, immunodeficiency diseases and cancer. This study retrospectively analyzed serum lymphocyte subsets and survival outcomes in intracranial germ cell tumors (iGCTs) patients.
Methods
Serum from145 iGCTs patients were collected and lymphocyte subsets were analyzed by flow cytometry. Clinical information was extracted from the database in Sun Yat-sen University Cancer Center and outcomes were confirmed through outpatient visits and telephone follow-up.
Results
76 patients were confirmed germinoma, 29 mixed germ cell tumor, 4 teratoma, 9 malignant teratoma, 6 endodermal sinus tumor, 20 choriocarcinoma and 1 embryonal carcinoma. 123(84.8%) patients received comprehensive treatments and 104(71.7%)reached complete or partial response. T lymphocytes (CD3+), cytotoxic T cells (CD3+CD8+ or Tc) and B lymphocytes(CD19+)decreased(p = 0.033, p = 0.011, and p < 0.001 respectively), while activated cytotoxic T lymphocytes(CD8+CD25+) increased(p < 0.001). Dynamic changes of activated Tc cells were associated with treatment patterns and evaluation of the disease(p = 0.038 and p = 0.043). Median survival was 45.81 months and patients of increased Tc cells, activated T helper cells(CD4+CD25+T cells or activated Th cells), activated Tc cells and normal CD4+/CD8+ ratio presented encouraging outcomes (p = 0.007;0.016;0.028;0.016 respectively). Nomogram and multivariate Cox Regression analysis indicated increased Tc cells was related to reduced risk death(p = 0.001,HR = 0.217 95%CI = 0.085—0.551).)
Conclusion
Implement of comprehensive treatments led to positive responses. Dynamic monitoring of serum lymphocyte subsets can be used as an auxiliary indicator for prognosis judgment.
Identification of key molecules and signaling pathways in GOLPH3 mediated colorectal cancer cells
Authors: Wang Wei , Wang Xiu-ying , Wang Mengshan , Zhang Liang 
Published: March 2022

Abstract
GOLPH3 is a peripheral membrane protein that is significantly associated with the development of cancer. However, the signaling pathways and mechanisms of GOLPH3 mediated cancer are still unclear. Here, our objective is to identify the essential molecules and signaling pathways by analyzing the RNA-seq data. The GSE196875 was produced by the Illumina NovaSeq 6000 (Homo sapiens). The KEGG and GO analyses indicated that axon guidance, signaling pathways regulating pluripotency of stem cells, and circadian entrainment are the major affected processes during the development of prostate cancer with GOLPH3 overexpression. Moreover, the ten interacting molecules were figured out including BMP2, RAC2, CACNA1C, PAX6, COL4A1, SPARC, GRIN1, CAV3, COL5A1, COL4A2. Thus, our study provides novel knowledge on the GOLPH3 affected cancer cells.
Triple-hit lymphoma in a 95-year-old patient: a case report and literature review
Authors: Tang Dongsheng , Chen Yue , Shi Yuye , Tao Hong , Tao Shandong , Zhang Quan'e , Ding Banghe , He Zhengmei , Yu Liang , Wang Chunling 
Published: March 2022

Abstract
Introduction: Diffuse large B cell lymphoma (DLBCL) is a highly heterogeneous tumor mainly occuring in the elderly, with approximately 40% of cases occurring in patients over 70 years old and this proportion will increase in the future. High grade B cell lymphomas (HGBL) with MYC and BCL2 and BCL6 rearrangements, so called triple-hit lymphomas (HGBL-THL), which usually presents a high-aggressive clinical behavior as well as to be associated with a very poor outcome. However, there are no standard treatment strategies for very elderly HGBL-DHL/THL patients, it still remains a challenge for clinicians. Case Presentaion: we report a case of a 95-year-old patient diagnosed with diffuse large B-cell lymphoma (HGBL-THL, non-germinal central type, stage IV group A, IPI 5 points, high risk group), coronary atherosclerotic heart disease (paroxysmal atrial fibrillation, cardiac insufficiency), hypertension, treated with chemotherapy, radiotherapy and targeted drug therapy obtained better outcome of keeping alive for 1 year. Conclusion: For very elderly HGBL-THL patients, a careful assessment of performance, nutritional status and comorbidities are needed before treatment. Novel therapies including immunotherapy, targeted therapy and chimeric antigen receptor T (CAR-T) cell therapy may provide benefit.
Redo of ventricular tachycardia ablation in a patient with ischemic cardiomyopathy using the newly omnipolar technology
Authors: Conti Sergio , Sabatino Francesco , Sgarito Giuseppe 
Published: March 2022

Abstract
BackgroundHigh-definition multielectrode mapping is becoming the cornerstone of complex ablation procedures. Differently from traditional bipolar electrograms that have susceptibility to directionality, Omnipolar Technology (OT) provides voltage, timing, and activation direction assessments independent of catheter orientation by incorporating simultaneously acquired EGM signals from multiple directions. Case presentationA 62 years-old male with ischemic cardiomyopathy underwent VT ablation guided by the EnSite X 3D mapping system. The first procedure was performed without the OT, while for the redo, OT was available. The substrate map performed by using the OT was much more defined, and the LPs map showed residual activity. Of great interest, an area noted as a dense scar during the first procedure appeared to be outside the dense scar region at the redo. Complete elimination of LPs in this region made the VT not inducible. ConclusionsHigh-definition multipolar mapping using the HD Grid can be rapidly and accurately performed during complex substrate mapping and ablation, such as post-ischemic VT. In particular, omnipolar mapping provide rapid, high-density, orientation-independent assessment of the underlying atrial substrate. This case report has a unique nature considering that the same patient underwent the index procedure without OT and redo with OT.
Microcredentials Training In Pharmacy Practice And Education: An Exploratory Study Of Its Viability And Pharmacists’ Professional Needs
Authors: Lok Peggy , Beyene Kebede , Awaisu Ahmed , Woods David , Kheir Nadir 
Published: March 2022

Abstract
Background: Microcredentials (MCs) are short courses that certify/recognise an individual's achievement of specific skills or knowledge. Schools of pharmacy could be well-placed to contribute to the continuing professional development (CPD) of pharmacists through the inclusion of MCs training in their programs. This study aimed to explore pharmacy professionals’ views on the need and viability of MC courses globally. Methods: Eleven semi-structured telephone interviews were conducted with pharmacy practitioners, policymakers, and academics across seven countries. The participants were selected using purposive sampling to explore information from varying pharmacy disciplines. Interviews were audio-recorded, transcribed verbatim, and analysed using a general inductive approach. Results: Participants regarded MCs in pharmacy as an innovative idea, well-suited to the increasingly technology-driven world. They believe MCs provide easily accessible means of skills and knowledge acquisition that fulfils the needs of the pharmacy profession. MCs were also perceived as an alternative pathway of meeting the requirements of traditional CPD programmes. Many participants believe universities are well-suited to provide MCs; however, numerous challenges such as recognition, time and resources have been identified as potential barriers to enrolment and implementation. Conclusions: This study provides an insight into the views of pharmacy practitioners and academics on MCs, and their potential utility in pharmacy education and practice. The findings should help in the development of MCs that could be utilised by pharmacy practitioners around the world for CPD purposes. This study comes at a time when alternative models of teaching and learning are being explored as a direct result of the COVID-19 pandemic.
Motion characteristics and energy efficiency of potential energy recycling system of telescopic handler
Authors: Xu Huairen , Liu Zhiqi , Wang Yao , Ge Lei 
Published: March 2022

Telescopic handler is combined of truck crane and forklift. Equipped with different accessories, the telescopic handler can achieve fork loading, lifting and other operations. Large amount of energy is needed to maintain lifting and lowering in large range with high frequency of the working device of telescopic handler. There is no energy recycling device for the working device that leads to the large dissipating of gravitational potential energy. In order to recycle the wasted energy, a three-chamber hydraulic cylinder boom system (TCCBS) is proposed. In this paper, the recycling principle is described. Then the energy consumption characteristics are analyzed. Further, the mode of TCCBS of telescopic handler is established and analyzed. Compared with the double chamber cylinder boom system (DCCBS), the single-action time of the boom is reduced 4.15 s. The velocity shock decrease from 0.12 m/s to 0.02 m/s at the beginning of the boom lowering process. The peak pressure decreased from 24 MPa to 21 MPa and the average pressure decreased from 17 MPa to 13 MPa during boom lifting process. The potential energy recycling of the working device is 139 kJ, which accounts for about 47.1 % of total potential energy. The cumulative output energy of TCCBS is 311 kJ, which is 92 kJ less than the DCCBS. The comprehensive energy saving efficiency is 22.3 %. The results show that the TCCBS can realize the potential energy recycling.