bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening for anti-neoplastic enzymes producing halophilic bacterial extract and their antioxidant activity due to carotenoid synthesis
Authors: Rathakrishnan Deepalaxmi , Gopalan Arunkumar 
Published: April 2022

Abstract
Out of 1127 halophilic bacteria, salt-loving microorganisms from different geographical locations in India isolated, twenty-eight strains were studied for antioxidant qualities for its usage in cancer medicines. The methanolic extracts were extracted and analyzed for anti-cancer effect through anti-neoplastic enzymes (L-asparaginase and L-glutaminase) screening, while antioxidant capabilities were investigated for fifteen isolates using both radical and non-radical assays.. The extracts also have enhanced radical scavenging ability against the stable 2,2'azinobis (3-ethylbenzothiazoline-6-sulfonic acid) disodium salt radical cation (86.34±0.007 mM TE/g extract), nitric oxide (64.79±0.004 μg/mL), phosphomolybdenum (1.69±0.024 mg AAE/g extract), ferric reducing power (28.19±0.012 mM Fe (II) E/mg) and showed better Ic50 values on 1,1-diphenyl- 2-picrylhydrazyl (5.48 mg/ml) assay. All of the studies has been done three times, and the results are significantly better than the standards utilized. Finally, four potent halophilic isolates from various antioxidant activities were chosen. These isolates could synthesize carotenoids that possessed anti-oxidant activities when grown in the halophilic medium was identified by UV/Visible spectra analysis. Secondary metabolite investigations using GC-MS study and LC-MS/MS analysis confirmed that isolated strains had the potential to produce compounds that might be used primarily as therapeutics. The enormous anti-oxidant potential of the strains Staphylococcus arlettae, Bacillus subtilis, B. tequilensis, and B. haynesii extracts could be used as a natural free radical scavenger to prevent oxidative-stress-related illnesses like cancer, neurodegeneration, as well as in the food safety markets in general.
Patient’s preferences on the use of Telemedicine during the Coronavirus disease 2019 (COVID-19) Pandemic. ONCOTELEMED study
Authors: Ribera Paula , Soriano Sandra , Climent Carla , Vilà Laia , Macias Ismael , Fernandez Luis , Giner Julia , Gallardo Enrique , Seguí Miquel Àngel , Pericay Carles 
Published: April 2022

Abstract
BACKGROUND
Since the beginning of the COVID-19 pandemic, the use of telehealth for cancer care was rapidly implemented without previous experience. Afterwards, few published studies have assessed patient’s perception with the use of telemedicine during this period. The main objective of ONCOTELEMD study was to evaluate the opinion of patients attended via telemedicine during the first quarter of 2020 in the Medical Oncology Service of Hospital Parc Taulí, Sabadell.
METHODS
646 patients were visited via telephone between March 13 and April 30 2020. A 12-question survey was presented to them between February 4 and April 19 2021. Statistical analysis was carried out using Chi-square test.
RESULTS
487 patients responded. Median age was 68 years [27–90]. 90% of patients were satisfied with the telephone visit and 60% would agree to switch some in-person to virtual visits in the future. In general, patients agreed to be informed via telehealth of radiological/analytical results but not of new oral or endovenous treatments. 75% of patients had a medium or low-null knowledge of new technologies. However, statistical significant differences were found according to age groups. 60% of patients younger than 50 years would prefer to have different technological tools to contact their oncologist whereas 58% of patients older than 70 years would prefer only telephone or video-calls.
CONCLUSIONS
In global, patients surveyed were satisfied with the virtual visit and believe that telehealth could have a role following COVID-19 pandemic. Nevertheless, it is necessary to individualize its use according to patient’s preferences and conditions.
Genome wide association study of grain hardness and novel Puroindoline alleles in common wheat
Authors: Wang Junyou , Yang Chenkang , Zhao Wenjia , Wang Ying , Qiao Ling , Wu Bangbang , Zhao Jiajia , Zheng Xingwei , Wang Juanlin , Zheng Jun 
Published: April 2022

Abstract
Grain hardness is a key determinant of wheat milling and end-use quality. Puroindoline genes (PINs) are the major genes responsible for wheat grain hardness, but other QTLs also contribute to this trait. Therefore, it is essential to identify loci associated with grain hardness and to the better describe the diversity of PINs in wheat. In this study, 287 wheat accessions representing 70 years of Shanxi Province wheat breeding were used to study grain hardness. Genome-wide association analysis (GWAS) was performed using the 15 K array, and the diversity of PIN alleles was investigated. Among the accessions there was a continuous distribution for grain hardness index (HI) with hard wheat being most common. GWAS identified nine significant marker-trait associations (MTAs), including that located on PINs, which explained 7.03–17.70% of phenotypic variation. Four MTAs on chromosome 2A, 2B, 5A, and 7A were novel loci. A total of 11 PIN alleles were detected, including a novel double-deletion allele of PIN genotype in Donghei1206. The most frequent genotypes were Pina-Dla/Pinb-Dlb (43.9%) and Pina-Dla/Pinb-Dip (18.8%), and both the frequency of Pina-D1a/Pinb-D1b and the HI value increased with breeding years. These results improve understanding of the genetics of grain hardness and help future molecular marker-assisted breeding for this important trait.
Trypanosoma cruzi antigenic proteins, shared with acute lymphoblastic leukemia and neuroblastoma
Authors: García Leticia Eligio , Guerrero Mariana Soria , Vázquez María del Pilar Crisóstomo , Acosta Víctor Alberto Maravelez , Campos Adrián Cortés , Jimenez Enedina 
Published: April 2022

Abstract
An inhibitory relationship has been reported between the incidence of some parasitic infections and the development of cancer. Some studies indicate that immunization with protein extracts of T.cruzi, the protozoan parasite that causes Chagas disease, prevents the appearance of tumors in 60% of mice injected with the murine lung carcinoma tumor line. The molecular bases of this process have not been elucidated, although the presence of some antigens present in tumor cells and on the surface of T.cruzi suggests an anti-parasitic immune response with an effective cross-reaction against cancer cells, hence the importance to identify the antigens involved and determine their potential as target cells in anticancer therapy. The general objective of this work is to determine the presence of antigenic proteins of T.cruzi, shared with cells in culture of ALL and NB, for this, polyclonal antibodies against T.cruzi were developed in rabbits, and the reactivity with protein extracts of cultured ALL and NB cells was determined, Likewise, the protein immunodetection of the different strains of T.cruzi was carried out with the anti-T.cruzi antibodies of the five strains. The study would also contribute to broadening the knowledge of immunological interactions between cancer and parasites in the future and therefore would broaden the panorama of new therapeutic strategies applied in oncology.
The Effect of Arterial Cannula Tip Position on Differential Hypoxia During Venoarterial Extracorporeal Membrane Oxygenation
Authors: Wickramarachchi Avishka , Burrell Aidan J.C. , Stephens Andrew F. , Šeman Michael , Vatani Ashkan , Khamooshi Mehrdad , Raman Jaishankar , Bellomo Rinaldo , Gregory Shaun D. 
Published: April 2022

Abstract
Background: The interaction between native left ventricular output and venoarterial extracorporeal membrane oxygenation (VA ECMO) flow may hinder perfusion of oxygenated blood to the aortic arch branches, resulting in differential hypoxia. Typically, the arterial cannula tip is placed in the iliac artery or abdominal aorta. However, the hemodynamics of an advanced arterial cannula tip have not been studied before. This simulation study aimed to investigate the effect of altering arterial cannula tip position on VA ECMO perfusion to the upper extremities.Methods: Computational fluid dynamics simulations were performed using a patient-specific geometry of the aorta and physiologically representative boundary conditions. Four arterial cannula tip positions (P1. common iliac, P2. abdominal aorta, P3. descending aorta and P4. aortic arch) were compared with different degrees of cardiac dysfunction and VA ECMO support (50%, 80% and 90% VA ECMO support).Results: P4 was able to supply oxygenated blood to the arch vessels at all support levels, while P1 to P3 only perfused the arch vessels with the highest level (90%) of VA ECMO support. Even during the highest level of support, P1 to P3 could only provide oxygenated VA ECMO flow at 0.11 L/min to the brachiocephalic artery, compared with 0.5 L/min at P4.Conclusions: Advancing the arterial cannula tip into aortic arch increased the perfusion of blood from VA ECMO to the aortic arch vessels at all simulated support levels. In contrast, the tip position used in standard clinical practice was predicted to result in differential hypoxia during 50% and 80% support with only a slight improvement at 90% support. Therefore, during VA ECMO, advancing the arterial cannula tip has the potential to improve upper body perfusion and reduce the risk of differential hypoxia.
CircCARM1 governs the miR-338-3p/TRAF3 axis to activate the IkBα/P65 signaling pathway, thus contributing to OGD/R-induced AC16 cell injuries
Authors: Yin Xuejiao , Chen Hongdan , Sun Guowei , Xu Yangxing , Wang Lingnan 
Published: April 2022

Abstract
Background: Myocardial cell injury is a key event in acute myocardial infarction (AMI). The involvement of circular RNA (circRNA) in cardiovascular disease has aroused much attention. The purpose of this study was to uncover the role of circRNA coactivator-associated arginine methyltransferase 1 (circCARM1) in cell model of AMI.Methods: Myocardial cells (AC16) were subjected to oxygen glucose deprivation/reoxygenation (OGD/R) to induce AMI models. Expression analysis of circCARM1, miR-338-3p and tumor necrosis factor receptor-associated factor 3 (TRAF3) was conducted using Real-time quantitative PCR (RT-qPCR). Cell viability, proliferation and apoptosis were assessed by CCK-8, EdU or flow cytometry assay. Oxidative stress was evaluated according to MDA level and SOD activity using commercial kits and the protein levels of NOX2 and SOD2 using western blot. The release of inflammatory factors was measured using ELISA kits. The predicted binding association between miR-338-3p and circCARM1 or TRAF3 was validated by pull-down assay or dual-luciferase reporter assay.Results: CircCARM1 was overexpressed in OGD/R-treated AC16 cells. OGD/R suppressed AC16 cell viability and proliferation and promoted apoptosis, oxidative stress and inflammation, while circCARM1 knockdown alleviated these injuries. MiR-338-3p was targeted by circCARM1, and miR-338-3p repression reversed the effects of circCARM1 knockdown. In addition, TRAF3 was a downstream target of the circCARM1/miR-338-3p axis, and TRAF3 reintroduction reversed the effects of miR-338-3p overexpression, thus recovering OGD/R-induced AC16 cell injuries. TRAF3 knockdown attenuated the activity of IkBα/P65 signaling pathway via modulating the miR-338-3p/TRAF3 axis in OGD/R-treated AC16 cells.Conclusion: CircCARM1 governed the miR-338-3p/TRAF3 axis to activate the IkBα/P65 signaling pathway, thus contributing to OGD/R-induced AC16 cell injuries.
Impacts of Koka Hydropower Dam on Benthic Macroinvertebrate Assemblages in the Awash River, Ethiopia
Authors: Getachew Melaku , Gebrie Geremew Sahilu , Mulat Worku Legesse , Mereta Seid Tiku , Kelly-Quinn Mary 
Published: April 2022

Abstract
Macroinvertebrate distribution and community structure in lotic water are influenced by a range of natural environmental variables and other anthropogenic factors, particularly related to the construction of dams. Hydropower dams can alter natural flow regimes, sediment transportation, hydrochemical water quality, channel morphology, water temperature, and nutrient cycles and physically obstruct the dispersal and the migration of macroinvertebrates and fish communities. The objective of this study was to evaluate the impacts of the Koka Hydropower Dam on benthic macroinvertebrate assemblage structures. Environmental, hydromorphological, and macroinvertebrate community data were collected along the course of the Awash River in the upper Awash basin. A total of 2305 macroinvertebrates assigned to 11 orders and 41 families were collected from 15 sites in the upstream, midstream, and downstream reaches. Ephemeroptera (32.2%), Diptera (24.6%), and Trichoptera (20.2%) represented 77% of the total abundances.The canonical correspondence analyses, multivariate test, showed that river velocity and phosphate concentration explained the majority of the variation (60%) in macroinvertebrate community structure among the three reaches possibly due to the dam’s impact in addition to other forms of impairment differences between reaches. The water quality categories of the river reach based on the Shannon diversity index at upstream, midstream, and downstream locations were high, moderate, and low, respectively. Based on the macroinvertebrate family-level information, this study found that the water quality degradation was most severe in the downstream reach. Overall, detection of the specific impacts of the dam is complicated by nutrient pollution in the river.
Prediction Model with External Validation for Early Detection of Postoperative Pediatric Chylothorax
Authors: Moza Rohin , NP Melissa Winder , Adamson Gregory T. , Ou Zhining , Presson Angela P. , Vijayarajah Senthuran , Goldstein Stephanie A. , Bailly David K. 
Published: April 2022

Abstract
Background: Earlier diagnosis of chylothorax following pediatric cardiac surgery is associated with decreased duration of chylothorax. Pleural fluid testing is used to diagnosis chylothorax which may delay detection in patients who are not enterally fed at time of chylothorax onset. Our aim was to develop and externally validate a prediction model to detect chylothorax earlier than pleural fluid testing in pediatric patients following cardiac surgery.Methods: A Multivariable logistic regression model was developed to detect chylothorax using a step-wise approach. The model was developed using data from patients <18 years following cardiac surgery from Primary Children’s Hospital, a tertiary-care academic center, between 2017 and 2020. External validation used a contemporary cohort (n=171) from Lucille Packard Children’s Hospital. Results: A total of 763 encounters (735 patients) were analyzed, of which 72 had chylothorax. The final variables selected were chest tube output (CTO) the day after sternal closure (dichotomized at 15.6 mL/kg/day, and as a continuous variable) and delayed sternal closure. The highest odds of chylothorax was associated with CTO on post sternal closure day 1 >15.6mL/kg/day (odds ratio 11.3, 95% CI 6,3, 21.3). The c-statistic for the internal and external validation datasets using the dichotomized CTO variable were 0.78 (95% CI:0.73, 0.82) and 0.84 (95% CI, 0.78, 0.9) and performance improved when using CTO as a continuous variable (OR 0.84, CI: 95% CI 0.80, 0.87) Conclusions: Using the models described, chylothorax after pediatric cardiac surgery may be detected earlier and without reliance on enteral feeds.
Molecular evidences show human papillomavirus type 124 can be trans-species infection in horses
Authors: Tong Panpan , Palidan Nuerlan , Song Xiaozhen , Jia Chenyang , Duan Ruli , Tian Shuyao , Yang Enhui , Pan Juanjuan , Kuang Ling , Jia Erken , Xie Jinxin 
Published: April 2022

Abstract
Human papillomavirus type 124 (HPV124), a member of betapapillomavirus, is associated with viral warts, actinic keratosis, and squamous cell carcinoma incidence in human. No reports of HPV124 infection in mainland China have been reported to date. In a survey of Chinese horses, we detected HPV124 via next-generation sequencing (NGS) in nasal swabs from 42% (39/93) of Thoroughbred horses, 7% (2/30) of the Akhal-Teke horses, and 17/50 aborted fetal lung tissues of Yili mares that were initially found to be negative for equid herpesvirus 1, which indicated the virus can be trans-species infection in different breeding horses, and suggested that this pathogen may potentially contribute to abortion incidence among horses. Further investigation showed that some workers involved in horse farm were positive for HPV124, which strongly suggested that horse-origin HPV124 strain may be transmissible from human workers to horses. Sequence analyses of E6 gene sequences from 18 Chinese HPV124 samples were performed, revealing that the 7 of 18 HPV124 strain identified both horses and human in the present study shared 100% sequence identity with previously published HPV124 isolate. Phylogenetic analyses based on E6 gene sequences in GenBank further revealed that the HPV124 isolate identified herein was closely related to the NJ3900 HPV124 reference strain, clustering together with the established Beta 1 HPV lineage. These results provide first evidence that HPV124 can infect horses, may function as a causative agent for abortions in Yili horses, and come from HPV124-positive humans laboring.
Inferring predictive genetic models and regulatory elements by deep learning of cross-species single-cell gene expression landscapes
Authors: Li Jiaqi , Wang Jingjing , Zhang Peijing , Wang Renying , Mei Yuqing , Sun Zhongyi , Fei Lijiang , Jiang Mengmeng , Ma Lifeng , E Weigao , Chen Haide , Wang Xinru , Fu Yuting , Liu Daiyuan , Wang Xueyi , Li Jingyu , Guo Qile , Liao Yuan , Yu Chengxuan , Jia Danmei , Wu Jian , He Shibo , Liu Huanju , Ma Jun , Lei Kai , Chen Jiming , Han Xiaoping , Guo Guoji 
Published: April 2022

Abstract
Despite extensive efforts to sequence different genomes, genetic models to interpret gene regulation and cell fate decisions are lacking for most species. Here, we performed whole-body single-cell transcriptomic analysis of zebrafish, Drosophila, and earthworm. We then mapped cell landscapes covering eight representative metazoan species to study gene regulation through evolution. With uniformly constructed cross-species datasets, we developed a deep learning-based strategy, Nvwa, to predict gene expression landscapes and decipher cis-regulatory elements (CREs) at the single-cell level. We systematically compared cell type-specific transcription factors (TFs) and CREs to reveal conserved genetic regulation among vertebrates and invertebrates. Our work provides a valuable resource and a novel strategy for studying regulatory language in diverse biological systems.
Maschinelle Gesichtserkennung im öffentlichen Raum
Authors: Braun Binder Nadja , Kunz Eliane , Obrecht Liliane 
Published: April 2022

Der Einsatz von maschineller Gesichtserkennung im öffentlichen Raum birgt die Gefahr einer gesellschaftlichen Massenüberwachung. Werden dabei biometrische Daten, die ein Individuum eindeutig identifizieren, genutzt, handelt es sich nach dem neuen Datenschutzgesetz um eine Bearbeitung besonders schützenswerter Personendaten. Die maschinelle Gesichtserkennung ist in der Schweiz regulatorisch kaum umrissen. Dabei sind Überlegungen dazu dringend geboten. Dies ergibt sich einerseits aus der Grundrechtsrelevanz der Thematik, andererseits fordern zivil-gesellschaftliche Organisationen ein entsprechendes Verbot. Auch die geplante EU-Regelung zur künstlichen Intelligenz, in der die Gesichtserkennung enthalten ist, sollte in den Erwägungen berücksichtigt werden. Vor diesem Hintergrund gibt der vorliegende Aufsatz einen Überblick über die im Zusammenhang mit maschineller Gesichtserkennung im öffentlichen Raum auftre-tenden Rechtsfragen und befasst sich mit der Frage der Notwendigkeit eines Verbots oder Moratoriums.
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L’utilisation de la reconnaissance faciale automatique dans l’espace public comporte le risque d’une surveillance de masse de la société. Si des données biométriques identifiant clairement un individu sont utilisées, il s’agit, selon la nou- velle loi sur la protection des données, d’un traitement de données personnelles sensibles. En Suisse, la reconnaissance faciale automatique n’est guère encadrée par la réglementation. Il est pourtant urgent de réfléchir à cette question. Cela résulte, d’une part, de l’importance de cette question en termes de droits fondamentaux et, d’autre part, de la volonté de certaines organisations de la société civile d’interdire une telle utilisation de ces données. La réglementation européenne prévue en matière d’intelligence artificielle, qui inclut la reconnaissance faciale, devrait également être prise en compte dans les réflexions. Dans ce contexte, le présent article donne un aperçu des questions juridiques qui se posent s’agissant de la reconnaissance faciale automatique dans l’espace public et se penche sur la question de la nécessité d’une interdiction ou d’un moratoire.
Using solar photovoltaic energy in urban water supply systems: a case study in Kayseri, Turkey
Authors: Sekmen Ufuk , Yildiz Omer Faruk , Yilmaz Mehmet , Ozdemir Ozgur 
Published: April 2022

AbstractSubmersible pumps, widely used in deep wells drilled to meet the water needs of users in urban areas, add an additional load to the conventional electricity grid infrastructure and are responsible for increasing the concentration of greenhouse gas emissions in the atmosphere. The main objective of this study is to investigate the technical and economic feasibility of using solar photovoltaic (PV) energy to reduce the net power drawn from the grid by submersible pumps in urban water supply systems. The Germiraltı catchment area (Kayseri, Turkey), which provides all of its energy needs from the city electricity grid, was chosen as the research area. Two different scenarios, ‘hybrid-powered water pumping system’ and ‘grid-connected PV system’, were analyzed and evaluated. A 1,620 kWp grid-connected PV system can inject 2,787.8 MWh/year of energy into the grid, which is more than the total annual energy need of the submersible pumps. The system operates with 85.7% performance ratio (PR), 19.6% capacity factor (CF) and 4.71 (h/d) final yield. The payback period of the system is calculated as 4.1 years. The results show that it is economically competitive to meet the energy needs of submersible pumps in urban water supply systems with solar PV energy.
River flow modelling for flood prediction using artificial neural network in ungauged Perkerra catchment, Baringo County, Kenya
Authors: Chebii Sally Jeruto , Mukolwe Micah Mukungu , Ong'or Basil Iro 
Published: April 2022

Abstract
Artificial neural network (ANN) modelling has been applied successfully in hydrology to predict future flows based on previous rainfall-runoff values. For a long time, flooding has been experienced in the surrounding areas of the Rift Valley lakes including Lake Baringo, fed by the River Perkerra, due to the rising water levels because of the above-normal rainfall season, resulting in massive socioeconomic losses. The study aims at predicting the occurrence of floods in River Perkerra using an ANN model with the input data being 417 consistent pairs of daily rainfall and discharge, and simulated runoff as the output. The model was trained, tested and validated producing a best fit regression with R2 of 0.951 for training, 0.938 for validation, 0.953 for testing giving an average of 0.949 indicating a close relationship between the input and output values. The overall best validation performance, RMSE, was 0.9204 m3/s indicating high efficiency of the FFNN model developed to predict floods. Flows greater than 14 m3/s, Q1, were the extreme flood events closely associated with socioeconomic losses. This prediction of Q1 value is crucial in the formulation and implementation of measures and policies by the County Government that will mitigate adverse impacts of predicted floods in the catchment.