bluuebunny/crossref_metadata_2025_split huggingface dataset:
“I’m not from a country, I’m from Australia.” Costumes, scarves, and fruit on their heads: The urgent need for Culturally Responsive Pedagogy when sharing diverse books with children.
Authors: Adam Helen , Byrne Matthew 
Published: August 2022

Abstract
Principles of diversity lie at the heart of the United Nations Convention on the Rights of the Child and are reflected in education policies worldwide. However, the translation of these principles into culturally responsive pedagogy (CRP) is problematic. This paper reports on a study conducted in kindergarten rooms of four long day care centres in Australia investigating educator practice when sharing culturally diverse children’s literature. Twenty-four educators and 110 children participated. Data was collected through field notes, semi-structured interviews, and video recordings. Findings show in the majority of book sharing in these centres the cultures, backgrounds, life experiences and funds of knowledge of children from underrepresented backgrounds were invisible. Further, educators’ practices were bereft of CRP and likely to demean and confuse those from underrepresented backgrounds and increase all children’s misconceptions of those different to themselves.
Skeletal muscle atrophy and short-term mortality in patients with acute exacerbation of idiopathic pulmonary fibrosis: An observational cohort study
Authors: Ito Yu , Anan Keisuke , Awano Nobuyasu , Kataoka Yuki , Johkoh Takeshi , Fujimoto Kiminori , Ichikado Kazuya , Tobino Kazunori , Tachikawa Ryo , Ito Hiroyuki , Nakamura Takahito , Kishaba Tomoo , Yamamoto Yosuke , Inomata Minoru , Izumo Takehiro 
Published: August 2022

Abstract
Skeletal muscle atrophy often complicates idiopathic pulmonary fibrosis (IPF). IPF patients frequently experience acute exacerbation (AE), but the association between skeletal muscle atrophy and mortality remains unknown in AE-IPF patients. Herein, cross-sectional areas of the erector spinae muscle (ESMCSA) and the pectoralis muscle (PMCSA) of AE-IPF patients were analysed via computed tomography. Primary outcome was 90-day mortality. Survival probability was estimated using the Kaplan–Meier method, and the log-rank test was used between the low and high groups of ESMCSA and PMCSA. We used multivariable Cox proportional-hazards models to evaluate the association between ESMCSA and PMCSA and prognosis. Among the 212 patients, 94 (44%) died during the observation period. The low ESMCSA group (< 25.6 cm2) had a significantly worse prognosis than the high group (≥ 25.6 cm2) (hazard ratio (HR) [95% confidence interval (CI)]: 1.52 [1.00–2.33], p = 0.049). Multivariable analyses showed that all-cause mortality was associated with low ESMCSA (model 1, adjusted HR [95% CI]: 1.59 [0.98–2.60]; model 2, 1.55 [0.95–2.56] and model 3, 1.67 [1.00–2.78]). A similar trend was observed between low PMCSA and poor prognosis (HR [95% CI]: 1.39 [0.88–2.20]). In conclusion, low ESMCSA is associated with high 90-day mortality in AE-IPF patients.
Disinfectant Stress Enhances Prokaryotic Symbiosis with Lysogenic Phages and Defense Systems Against Detrimental Phage Infection
Authors: Huang Dan , Chen Juhong , Shi Zhou Jason , Liu Jingqing , Alvarez Pedro J.J. , Yu Pingfeng 
Published: August 2022

Abstract
Background
Mutualistic symbiosis is critical for microbial adaptation to stress and microbiome functions. Recent progress in metagenomics has advanced understanding of prokaryote-phage symbiosis and the ecological and evolutionary significance of viromes. However, it remains elusive how prokaryotic antiviral systems respond to symbiosis with lysogenic phages and its implications to microbial ecosystem services. Microbiomes in drinking water distribution systems (DWDS) under disinfectant stress are an important platform to study the ecological drivers and effects of prokaryote-phage symbiosis.
Results
Enhanced prokaryote-phage mutualism was observed in the microbiome of 7 DWDS under disinfectant stress relative to 5 control DWDS without disinfection. The relative abundance of lysogenic phages increased in microbiomes in DWDS with residual chlorine. Prokaryote-phage linkage analysis revealed that residual chlorine enriched phages that reproduced in disinfectant-tolerant prokaryotes, and selected for broad-host-range phages that could propagate using multiple hosts. Moreover, the virome under disinfectant stress harbored high levels of auxiliary metabolic genes (AMGs) encoding reductase, which could alleviate oxidative stress. Various prokaryotic antiviral systems were elevated under disinfectant stress, particularly the Restriction-Modification (RM) and CRISPR-Cas systems. Some enriched RM and CRISPR-Cas systems were linked to lysogenic phages and prophages, inferring that these antiviral systems are compatible with phage infections with mutualistic potential. In addition, RM systems harbored in lysogenic phages and prophages were enriched in the disinfected DWDS virome. This apparently benefited lysogenic phages to evade prokaryotic antiviral systems through RM-mediated methylation of phage DNA. Transduction of antiviral genes would also empower the hosts with additional capability to defend against secondary infections by lytic phages.
Conclusion
This metagenomic study infers that sublethal residual disinfectants can enhance prokaryote-phage mutualism, and enrich some prokaryotic antiviral systems to defend against detrimental (lytic) phage infection. Our study advances understanding of the development, maintenance and overlooked implications of prokaryote-phage mutualism, which offers new perspectives for microbiome adaptation under environmental stress.
Heavy Metals Accumulation in Dragonflies (Insecta: Odonata) and Their Microhabitats in District Mardan, Khyber Pakhtunkhwa, Pakistan
Authors: Khan Khurshaid , Shams Nida , Rahman Gauhar , Shah Safeer Ullah , Ullah Mujeeb , Jamal Muhsin , Badshah Noor , Zia Syed Ahmad 
Published: August 2022

Abstract
Dragonflies (Odonata: Insecta) are bio-physical environmental indicators and limited information of this important insect group are reported from Khyber Pakhtunkhwa, province of Pakistan. Therefore, the present study was attempted to find out the bionomics of dragonflies and heavy metals concentration in their habitats in District Mardan, Khyber Pakhtunkhwa, Pakistan. During the present study total of 1400 adult dragonflies’ specimens were collected from May to October, 2018 in three tehsils of District Mardan. Orthetrum anceps (N = 209, 14.93%) was the most abundant species followed by O. pruinusum neglectum (N = 177, 12.64%) and Trithemis festiva (N = 141, 10.07%). Diversity was highest for Katlang as compared to other tehsils. Dragonflies’ peak aggregation was observed in July and August. O. pruinusum neglectum positively correlated (p < 0.05) with monthly total rainfall. Dragonfly species were sampled in abundance from all surveyed elevation (< 361 to 953m), on agriculture land and q of lithological unit. Significant difference in heavy metals (Mg, Zn, Fe, Cu, Cr and Pb) accumulation in water bodies and sediments was observed among studied tehsils. Metals accumulation significantly varied among body parts of different species of dragonflies. One of the first studies to report species composition, spatial distribution and effects of heavy metals contamination on dragonflies in district Mardan.
Anisotropic Wood-Water Interactions Determined by Gravimetric Vapor Sorption Experiments
Authors: Sánchez-Ferrer Antoni Antoni , Engelhardt Max , Richter Klaus 
Published: August 2022

Abstract
Gravimetric vapor sorption experiments were performed on beech wood samples to determine the directional permeability, diffusion and sorption coefficients in the three orthotropic wood directions. Dynamic Vapor Sorption (DVS) experiments allowed for the direct evaluation of the diffusion coefficient from the analysis of the kinetic sorption profile using a double stretched exponential model with values ranging from 0.10ꞏ10− 10 to 1.52ꞏ10− 10 m2/s and depending on the wood direction of the sample and the RH-values. Moisture sorption isotherms (MSIs) were constructed and fitted to a modified Guggenheim-Anderson-de Boer model, which allowed for the calculation of the sorption coefficient which was found to be between 2.4 and 3.0 mol/(m3ꞏPa). Dynamic Vapor Transport (DVT) experiments were performed to calculate the permeability coefficient from the vapor flow rate and it ranges between 0.56ꞏ10− 10 and 4.38ꞏ10− 10 mol/(mꞏsꞏPa) as a function of the flow direction and RH conditions. These results indicate that such an experimental approach is suitable for determining wood-moisture interactions.
The influence of sex and sexual orientation disparities on eating disorder behaviours and suicidality in a large Chinese adolescent’s sample
Authors: Wang Yuanyuan , Ma Zhihao , Lu Su , Duan Zhizhou , Wilson Amanda , Jia Yinwei , Yang Yong , Chen Runsen 
Published: August 2022

Abstract
Background and aims: Disordered eating leads to impaired well-being including physical health, psychosocial functions, and medical complications. There are insufficient studies have focused on the influence of sex and sexual orientation disparities on disordered eating. This study aimed to investigate disordered eating among male and female adolescents with different sexual orientations in a school sample.Method: Data was analyzed from 11,440 Chinese school adolescents with a mean age of 14.74 years (SD = 1.46). Self-reported data was gathered on sociodemographic information including sexual orientation, eating disorder (ED) behaviours, health factors (Self-rated health, cognitive function), mental health factors (depression, anxiety, suicidal ideation, non-suicidal self-injurious behaviour), and social functioning (school bully victimization, and school bully perpetration). Logistic regression models were used to estimate risk factors associated with disordered eating.Results: Overall, the prevalence of dietary restriction presented sex disparities—a total of 2,610 female adolescents (49.41%) reported having dietary restriction, while 2,064 male adolescents (33.69%) reported having dietary restriction. In the unidentified sexuality group adolescents tended to be less likely to engage in disordered eating. Using heterosexual orientation as reference group, being unidentified sexuality individuals was associated with lower risk in dietary restriction and purging in both male and female adolescents. Using heterosexual orientation as reference group, female sexual minority groups were at high risk of disordered eating, with bisexual orientation and homosexual orientation had a higher likelihood of objective binge eating. Conclusions: The results revealed significant sex and sexual orientation differences across the prevalence of ED behaviours. The study indicated that the sexual awareness period could be critical for understanding adolescents’ eating behaviours. It is important to guide adolescents to healthy eating during the process of sexual awareness. Considerations should be implemented by clinicians when creating interventions for disordered eating behaviours among different sex and sexual orientation groups.
Crystal structure and anticancer screening of 2-(4-acetylphenylcarbamoyl)phenyl acetate
Authors: Mohamed-Ezzat Reham A. , Kariuki Benson M. , S Aladdin M. 
Published: August 2022

Abstract
Aspirin-based 2-(4-acetylphenylcarbamoyl)phenyl acetate (3) has been synthesized through the reaction of 2-(chlorocarbonyl)phenyl acetate and 1-(4-aminophenyl)ethanone in the presence of triethyl amine. Assessment of anti-proliferative properties of the compound on NCI 60-cell line panel revealed remarkable to moderate anti-cancer activity. Single-crystal x-ray diffraction and spectroscopic techniques were used to validate the chemical structure of the compound.
Phylogenetic analysis of Bamboo species using Internal Transcribed Spacer sequence
Authors: Khanday Ashiq Hussain , Gawande Prashant A. , Badroo Irfan Ashraf , Wagay Nasir Aziz , Abdi Gholamreza , Tarighat Maryam Abbasi 
Published: August 2022

Abstract
Need for the use of molecular technique in Bamboo is very useful for better resolution of phylogenetic studies. In this study the present investigator has used (ITS1, 5.8S rRNA and ITS2) region of Bamboo species to construct the phylogenetic tree of 18 tropical bamboo species. Ribosomal internal transcribed spacer (ITS) sequences are commonly used for phylogenetic reconstruction because they are highly reiterated as components of rDNA repeats, and hence are often subject to rapid homogenization through concerted evolution. All Bamboo species studied were collected from different regions of India and were further subjected for the process of DNA sequencing. A phylogenetic analysis of the 18 Bamboo species under six different genera were performed using neighbor-joining and maximum-likelihood methods. These findings are further correlated with the morphological character based dendrogram of these species. The implications of the findings are discussed. Present study has shown a close relation between the two genera Dendrocalamus and Bambusa. However, dendrogram based on the morphological characters showed the intergenic and interspecific clustering of Bamboo species. Keeping in view all the possible outcomes the current research has focused to produce some useful conclusion of the two data sets. Molecular data was used to give the resolution for anomalies encountered which prompted the refinement of morphological character analysis. Although the molecular data helped to resolve the phylogenetic issues in Bamboos still the dependability of combined data sets (molecular and morphological) would be of very importance. This work has focused to reveal the phylogenetic relation among Bamboo species.
Dancing with sterols: can antibiotic-sterol interactions explain the selectivity of aromatic analogues of amphotericin B?
Authors: Borzyszkowska-Bukowska Julia , Czub Jacek , Szczeblewski Paweł , Laskowski Tomasz 
Published: August 2022

Abstract
Aromatic heptaene macrolides (AHMs) belong to the group of polyene macrolide antifungal antibiotics. Members of this group were the first to be used in the treatment of systemic fungal infections. Amphotericin B (AmB), a non-aromatic representative of heptaene macrolides, is of significant clinical importance in the treatment of internal mycoses. It includes the all-trans heptaene chromophore, whereas the native AHMs contain two cis-type (Z) double bonds within the chromophore system. Lately we have proven that it is possible to obtain AHMs’ stable derivatives in the form of all-trans (AmB-type) isomers by photochemical isomerization. Our further studies have shown that such alteration leads to the improvement of their selective toxicity in vitro. Computational experiments carried out so far were only an initial contribution in the investigation of the molecular basis of the mechanism of action of AHMs and did not provide explanation to observed differences in biological activity between the native (cis-trans) and isomeric (all-trans) AHMs. Herein, we presented the results of two-dimensional metadynamics studies upon aromatic AmB analogues regarding preferable binary antibiotic/sterol complexes orientation, as well as more detailed research on the behavior of their alkyl-aromatic side chain in cholesterol- or ergosterol-enriched lipid bilayers.
Degradation of lignin in different lignocellulosic biomass by steam explosion combined with microbial consortium treatment
Authors: Zhang Wen , Diao Chenyang , Wang Lei 
Published: August 2022

Abstract
The difficulty of degrading lignin is the main factor limiting the high-value conversion process of lignocellulosic biomass. Biodegradation of lignin has attracted much attention because of its strong environmental friendliness, but it still faces some dilemmas such as slow degradation speed and poor adaptability. The microbial consortia with high lignin degradation efficiency and strong environmental adaptability were obtained in our previous research. In order to further increase the lignin degradation efficiency, this paper proposes a composite treatment technology of steam explosion combined with microbial consortium degradation to treat three kinds of biomass. We measured the lignin degradation efficiency, selectivity value (SV) and enzymatic saccharification efficiency. The structural changes of the biomass materials and microbial consortium structure were also investigated. The experimental results showed that after 1.6 MPa steam explosion treatment, the lignin degradation efficiency of the eucalyptus root reached 35.35% on the 7th d by microbial consortium. At the same time, the lignin degradation efficiency of the bagasse and corn straw treated by steam explosion followed by microbial biotreatment was 37.61% and 44.24%, respectively, after only 7 days biotreatment. The microbial consortium also showed strong selectivity degradation to lignin. The composite treatment technology can significantly improve the enzymatic saccharification efficiency. Saccharomycetales, Ralstonia and Pseudomonadaceae were the dominant microorganisms in the biomass degradation systems. It was proved that the combined treatment technology of steam explosion and microbial consortium degradation could overcome the drawbacks of traditional microbial pretreatment technology, and can facilitate the subsequent high-value conversion of lignocellulose.
Antioxidative stress protein SRXN1 can be used as a radiotherapy prognostic marker in prostate cancer patients
Authors: Wang Xing , Yu Jiandi , Wen Huali , Yan Junfeng , Peng Kun , Zhou Haiyong 
Published: August 2022

Abstract
Purpose
To explore the mechanisms that affect the radiosensitivity of prostate cancer and to search for radiotherapy outcome-related prognostic markers in prostate cancer patients through bioinformatics analysis.
Methods
The cancer cell data set GSE192817 and TCGA PRAD data set were downloaded from GEO database and UCSC Xena database for differential analysis, and biological function analysis of differential genes was performed (p < 0.05). Five gene sets were obtained from the MsigDB database, and ssGSEA and GSVA algorithms were used to quantitatively score cell line samples and patients. According to the expression of differential genes, the radiotherapy patients were divided into high and low expression groups for survival analysis, and the correlation between the expression of the prognostic gene SRXN1 and the GSVA score of oxidative stress response was analyzed. The differences between patients with high and low expression of SRXN1 were compared, and the patients were divided into two groups with high and low GSVA scores in response to oxidative stress and survival analysis was performed using the survival package.
Results
A total of six cancer cell lines after different radiotherapy treatment times were detected from GSE192817, and 114 differentially expressed genes were obtained, which were mainly related to nuclear membrane reassembly (p = 0.038) and cell adhesion (p = 0.042). Comparison of DNA damage-stimulated ssGSEA in different cell lines showed that, with the exception of the FaDu cell line, all cell lines treated with proton radiotherapy had higher response scores than those treated with X-ray radiotherapy. Comparing the three DNA damage-related functional GSVA results in TCGA PRAD patients showed that X-ray therapy had higher scores than patients without radiotherapy. Wilcoxon test screened 8 differential genes common to PC3 and DU145 cell lines. Survival analysis found that the high expression of TOR1B and SRXN1 was not conducive to the radiotherapy outcome of patients. Correlation analysis showed that SRXN1 gene expression was significantly negatively correlated with oxidative stress response GSVA score (PCC=-0.1536, p = 0.0003), and the oxidative stress response GSVA score was significantly higher in patients with low SRXN1 expression (p = 0.0072) ), high oxidative stress response scores in patients were beneficial to radiotherapy outcomes.
Conclusions
The prognostic marker SRXN1 of prostate cancer screened by bioinformatics plays an antioxidant stress role in prostate cancer cell lines after radiotherapy, which is associated with poor prognosis of radiotherapy outcomes in prostate cancer patients.
Assessing the Relationship Between Zero-Dose Communities and Access to Selected Primary Healthcare Services for Children and Pregnant Women in Emergency Settings
Authors: Suprenant Mark Paladin , Nyankesha Elevanie , Moreno-Garcia Ralfh , Buj Valentina , Yakubu Ahmadu , Shafique Fouzia , Zaman Muhammad Hamid 
Published: August 2022

Abstract
In this study the authors examine the relationship between “zero-dose” communities and access to other healthcare services. This was done by first ensuring the first dose of the Diphtheria Tetanus and Pertussis vaccine was a better measure of zero-dose communities than the measles-containing vaccine. Once the best variable was selected, it was used to examine the association with access to primary healthcare services for children and pregnant women residing in the Democratic Republic of Congo, Afghanistan, and Bangladesh, each of which are currently experiencing emergencies of various contexts and degrees. These services were divided into: a) unscheduled healthcare services such as birth assistance as well as seeking care and treatment for diarrheal diseases and cough/fever episodes and b) other scheduled health services such as antenatal care visits and vitamin A supplementation. Using the most recent Demographic Health Survey data from each country (2014: Democratic Republic of Congo, 2015: Afghanistan, 2018: Bangladesh), data was analyzed via Chi Squared analysis or Fischer’s Exact Test. If results were significant, a univariate linear regression analysis was performed to examine if the noted association was linear. While the linear relationship observed between children who had received the first dose of the Diphtheria Tetanus and Pertussis vaccine (the reverse to zero-dose communities) and coverage of other vaccines was expected, the results of the regression analysis depicted an unexpected split in behavior between scheduled (and birth assistance) and unscheduled illness treatment services. For scheduled and birth assistance health services, a linear relationship was generally observed. Meanwhile, for unscheduled services associated with infectious disease treatments, a linear relationship was generally not observed. While in our study it does not appear that first dose of the Diphtheria Tetanus and Pertussis vaccine, the best proxy of zero-dose communities, can be used to predict (at least in a direct linear manner) access to some primary (particularly infectious treatments) healthcare services in emergency/ humanitarian settings, it can serve as an indirect measure of health services not associated with the treatment of childhood infections such as antenatal care, skilled birth assistance, and to a lesser degree even vitamin A supplementation.