bluuebunny/crossref_metadata_2025_split huggingface dataset:
Newborn Screening for SMA – Can a Wait-and-See Strategy be Responsibly Justified in Patients With Four SMN2 Copies?
Authors: Blaschek Astrid , Kölbel Heike , Schwartz Oliver , Köhler Cornelia , Gläser Dieter , Eggermann Katja , Hannibal Iris , Schara-Schmidt Ulrike , Müller-Felber Wolfgang , Vill Katharina 
Published: July 2022

Background: Early treatment after genetic newborn screening (NBS) for SMA significantly improves outcomes in infantile SMA. However, there is no consensus in the SMA treatment community about early treatment initiation in patients with four copies of SMN2. Objective: Approach to a responsible treatment strategy for SMA patients with four SMN2 copies detected in newborn screening. Methods: Inclusion criteria were a history of SMA diagnosed by NBS, age > 12 months at last examination, and diagnosis of four SMN2 copies at confirmatory diagnosis. Results: 21 patients with SMA and four SMN2 copies were identified in German screening projects over a three-year period. In three of them, the SMN2 copy number had to be corrected later, and three patients were lost to follow-up. Eight of the fifteen patients who were subject to long-term follow-up underwent presymptomatic therapy between 3 and 36 months of age and had no definite disease symptoms to date. Five of the other seven patients who underwent a strict follow-up strategy, showed clinical or electrophysiological disease onset between 1.5 and 4 years of age. In two of them, complete recovery was not achieved despite immediate initiation of treatment after the onset of the first symptoms. Conclusion: A remarkable proportion of patients with four copies of SMN2 develop irreversible symptoms within the first four years of life, if a wait-and-see strategy is followed. These data argue for a proactive approach, i.e., early initiation of treatment in this subgroup of SMA patients.
Pengaruh Experiental Marketing Terhadap Kepuasan Pelanggan di Kebumen Barbershop
Authors: Wardoyo Eko 
Published: July 2022

Penelitian ini dilakukan pada pelanggan Kebumen barbershop dengan tujuan untuk mengetahui dan menganalisis seberapa besar pengaruh experiental marketing Sense, Feel, Act, Relate, Think baik secara parsial maupun simultan terhadap kepuasan pelanggan. Jumlah sampel sebanyak 100 responden dengan teknik pengambilan metode non probability sampling Metode penelitian bersifat analisis kuantitatif, data diperoleh berdasarkan jawaban kuesioner. Berdasarkan dari hasil penelitian variabel yang diteliti, menunjukkan ternyata ada 4 variabel dari dimensi experiential marketing yang berpengaruh signifikan terhadap kepuasan pelanggan yakni sense, feel, act dan relate. Sedangkan variabel think menunjukkan tidak ada pengaruh siginifikan terhadap kepuasan pelanggan. Sedangkan uji simultan diperoleh variabel sense, feel, think, act dan relate secara bersama sama berpengaruh signifikan terhadap kepuasan pelanggan.
Technical Guidance for Increasing Production of Cow Manure-Based Organic Fertilizer in Farmer's Groups in Konda District
Authors: Hafid Harapin , Midranesiah  , Ananda Siti Hadrayanti 
Published: July 2022

This technical guidance activity aims to teach how to process organic fertilizer based on cow dung which is mostly produced by the community's livestock, so that it can reduce the cost of inorganic fertilizer and be more profitable. This activity was carried out in farmer groups in Morome and Alebo Villages, Konda District, South Konawe Regency. The method of activity is carried out by: (1) lectures and discussions on materials and methods of processing organic fertilizers, (2) demonstrations and guidance on the selection of materials and processing of cow dung-based organic fertilizers, (3) packaging and marketing methods for livestock manure-based organic fertilizers. cow. The counseling material presented received a positive and satisfactory response from the participants. In general the participants wanted to adopt the training materials and were very active in the technical guidance. It was concluded that the participants were very interested in applying the guidance materials taught and producing them commercially. The participants hoped that their village would often be given agricultural and livestock guidance and counseling activities.
Pengaruh Range of Motion (ROM) Aktif Kaki terhadap Sensitivitas Kaki pada Pasien Diabetes Millitus Tipe II di Wilayah Kerja Puskesmas Masbagik
Authors: Purnamawati Dewi , Kresnawati Yulia Tri , Mawaddah Ely , Sentana Aan Dwi 
Published: July 2022

In the era of globalization there is a shift from infectious diseases to non-communicable diseases, more and more degenerative diseases, one of which is Diabetes Millitus. DM is a metabolic disease characterized by increased blood sugar levels due to insulin secretion disorders. Hyperglycemia and metabolic disorders in DM can cause complications, one of which often occurs in DM patient is nerve damage or peripheral neuropathy so that it can cause a decrease in foot sensitivity. The purpose of this study was to determine the influence of foot active range of motion (ROM) on the sensitivity of foot in patients Diabetes Millitus type II in the working area of primary health care Masbagik year 2021. This study is a pre-exsperimental research design with One Grup Pretest-Posttest. Approach. The sample in this study is patients with Diabetes Millitus type II in the working area of primary health care Masbagik 22 people sampel. Sampling technique using Purposive sampling. Statistical test results show that there is an effect of active foot Range Of Motion (ROM) on foot sensitivity in patients Diabetes Millitus type II with P test results, namely p value = 0.000 < a = 0.05, where the null hypothesis (H0) is rejected or the alternative hypothesis (Ha) accepted, It can be concluded that there is an effect of foot active range of motion (ROM) on the sensitivity of foot in patients Diabetes Millitus type II. Respondents are advised to always do Range Of Motion (ROM) active foot of the foot to maintain foot sensitivity. If foot sensitivity is not maintained, the roots experience impaired foot sensitivity which can cause diabetic ulcers or diabetic foot.
EDUCATION OF FILL MY PLATE COMIC ON OVERWEIGHT ELEMENTARY SCHOOL STUDENTS
Authors: Darni Joyeti , Wahyuningsih Retno , Utama Lalu Juntra 
Published: July 2022

Background: In the lifestyle shift in Indonesian society, consumption patterns are high in calories, high in simple sugars, and low in fiber and not balanced with sufficient physical activity. Nutrition education can prevent the increasing prevalence of obesity and overweight. An approach to the client is needed in solving problems to change the pattern of life to be healthier and better. Students in Elementary schools tend to prefer illustration books in realistic or cartoon form. Comics have an excellent visual media function in helping to increase knowledge of balanced nutrition, and it is hoped that this will influence the behavior of balanced nutrition. The purpose is to determine the effect of providing comic education Fill My Plate on the energy of elementary school children with excess nutritional status. Methods: This quasi-experimental study involved 60 students who were divided into treatment groups by providing education on comics Fill My Plate and a control group giving education without comics Fill My Plate energy data analysis in both groups using paired t-test. Research Result: The results showed an influence between the energy before and after giving education on comics Fill My Plate in the treatment group (P = 0.000), while the control group had no effect (p = 0.897). Conclusion: Providing education using comics Fill My Plate affected reducing energy in elementary school students who have more nutritional status.
Problematika Komunikasi Mahasiswa dan Dosen Pada Perkuliahan Daring di Masa Pendemi
Authors: Anrial  , Yansah Savri , Mulkati  
Published: July 2022

Pelasanaan perkuliah online / daring selama pendemi Covid-19 yang sudah berjalan selama lebih kurang dua tahun sangat berpengaruh sekali terhadap proses komunikasi antara mahasiswa dan dosen, khususnya mahasiswa angkatan tahun 2019, karena proses perkuliahan ofline hanya dilakukan beberapa pertemuan di semester satu, di saat keluar intruksi pemerintah untuk melaksanakan perkuliahan daring dengan tujuan untuk mengurangi resiko penyebaran covid-19. Sesuai dengan keputusan pemerintah di atas, Institut Agama Islam Negeri Curup juga mengikuti dan melakukan proses perkuliahan secara daring dan berlaku utuk semua mahasiswa. Karena belum ada ketentuan bagaimana proses yang tepat dalam melakukan perkuliahan daring, hal ini akan berpengaruh terhadap kemampuan mahasiwa dalam memahami materi perkuliahan karena proses komunikasi antara mahasiswa dan dosen selama perkuliahan daring sering terjadi hambatan. Metode penelitian yang digunakan untuk mendapatkan data ini adalah penelitian kualitatif dengan pendekatan studi kasus, karena masalah yang di angkat adalah sebuah problema yang terjadi antara mahasiswa dan dosen selama perkuliahan daring. Dari hasil penelitian di peroleh berhubungan dengan penggunaan aplikasi yang di gunakan oleh dosen selama perkuliahan daring adalah media Whatshap sebanyak 7%, Setelah itu menggunakan Zoom Meeting Sebanyak 2 %, Google Meet Sebanyak 1,5 % dan Clas Room sebanyak 0,5. Penggunaan media whatshap merupakan cara yang praktis yang dilakukan karena penggunaan aplikasi lain lebih banyak hambatan yang disebabkan oleh kekuatan sinyal yang berada di tempat masing-masing mahaiswa, namun penggunaan media whatshap selama perkuliahan daring, dosen kebanyakan memberikan tugas kepada mahasiwa sehingga penjelasna materi setiap mata kuliah tidak dilakukan dan mahasiswa secara mandiri hanya melaksanakan dan menyelesaikan tugas yang di berikan dosen sesuai dengan mata kuliah yang mereka ikuti.
EFEKTIVITAS SENAM OTAK (BRAIN GYM) DALAM MENURUNKAN TINGKAT KEJENUHAN PERKULIAHAN DARI RUMAH SELAMA MASA PANDEMI COVID – 19
Authors: Kurniawan Dede Tri , Maryanti Sri 
Published: July 2022

Fenomena Study From Home di masa Pandemi Covid-19 seringkali membuat mahasiswa merasa jenuh. Sudah lebih dari dua bulan sejak diberlakukannya kebijakan untuk work from home. Segala kegiatanpun dialihkan secara online dari rumah, termasuk kegiatan perkuliahan. Selama beberapa lama di rumah saja, wajar jika rasa jenuh dan bosan mulai dirasakan. Fokuspun mulai terganggu yang berujung pada sulitnya memahami materi saat kuliah online. Dari hal itu peneliti meurumuskan masalah bagaimana efektivitas senam otak (brain gym) menurunkan tingkat kejenuhan mahasiswa. Penelitian ini bertujuan untuk mengetahui apakah senam otak (brain gym) efektif dalam menurunkan tingkat kejenuhan belajar (burnout) pada mahasiswa calon guru Raudhatul Athfal (RA). Solusi yang bisa dilakukan agar mahasiswa menjadi lebih aktif dan lebih bisa dengan mudah menerima pelajaran yang disampaikan oleh dosen salah satunya yaitu dengan melakukan senam otak atau bisa disebut dengan brain gym. Dalam penelitian ini peneliti merumuskan masalah yaitu: apakah senam otak efektif dalam menurunkan tingkat kejenuhan mahasiswa dan bagaimana cara senam otak dalam menurunkan kejenuhan mahasiswa calon guru Raudhatul Athfal (RA)? Jenis Penelitian ini adalah penelitian deskriptif kualitatif. Pengumpulan data dilakukan dengan cara observasi, wawancara dan dokumentasi. Hasil penelitian menunjukan bahwa brain gym mampu menurunkan tingkat kejenuhan mahasiswa selama perkuliahan masa pandemi covid-19. Selain mahasiswa tidak jenuh mahasiswa juga lebih konsentrasi fokus dan membantu meningkatkan motivasi. Beberapa gerakan senam otak dapat membantu efektivitas perkuliahan dalam jaringan sehingga meningkatkan fokus konsentrasi dan pemahaman, membantu meningkatkan konsentrasi, meningkatkan konsentrasi dan fokus, dan membantu meningkatkan motivasi.
ANALISIS MOTIVASI BELAJAR SISWA KELAS X IPA TERHADAP MATA PELAJARAN FISIKA DI SMA N 6 MUARO JAMBI
Authors: Putri Wita Ardina , Meliza Wella , Astuti Yuli 
Published: July 2022

Penelitian ini merupakan penelitian kuantitatif. Penelitian ini bertujuan untuk menganalisis serta mengetahui motivasi belajar peserta didik kelas X di SMA N 6 Muaro Jambi terhadap mata pelajaran fisika. Penelitian ini menggunakan metode penelitian survey. Dimana penelitian ini dilakukan dengan cara menyebarkan kuisioner atau yang biasa disebut dengan angket secara online kepada peserta didik. Setelah melakukan penyebaran angket dan memperoleh data yang diinginkan, selanjutnya peneliti mengelolah data menggunakan spss dengan teknik desktiprif. Hasil yang diperoleh dari penelitian ini kemudian dijelaskan melalui deskriptif kuantitatif dalam bentuk tabel yang terdiri dari presentase motivasi belajar peserta didik. Adapun perolehan presentase motivasi belajar fisika dari peserta didik kelas X di SMA N 6 Muaro Jambi yaitu 0% yang dapat dikatakan tidak baik, 5% cukup baik, 78,3 % baik, dan 16,7% sangat baik. Berdasarkan hasil prosentase motivasi belajar peserta didik di SMAN 6 Muaro Jambi, maka dapat dikatakan bahwa motivasi belajar peserta didik di SMAN 6 Muaro Jambi terhadap mata pelajaran fisika sudah baik..
Peningkatan Pengetahuan Petugas Ahli Teknologi Laboratorium Medis (ATLM) di Klinik Utama Bunga Emas Terkait Manajemen Laboratorium
Authors: Parwati Putu Ayu 
Published: July 2022

Manajemen laboratorium merupakan usaha untuk mengelola laboratorium. Suatu laboratorium dapat dikelola dengan baik sangat ditentukan oleh beberapa faktor yang saling berkaitan satu dengan lainnya. Oleh karena itu, manajemen laboratorium adalah salah satu bagian yang tidak dapat dipisahkan dengan kegiatan laboratorium sehari-hari. Untuk menghasilkan pemeriksaan laboratorium yang dapat dipercaya/ bermutu, maka setiap tahap pemeriksaan laboratorium harus dikendalikan. Pengendalian setiap tahap ini untuk mengurangi atau meminimalisir kesalahan yang terjadi di laboratorium. Mutu laboratorium klinik meliputi mutu hasil pemeriksaan dan mutu layanan. Kegiatan ini bertujuan untuk meningkatkan pengetahuan dengan memberikan informasi kepada ATLM terkait manajemen laboratorium. Metode kegiatan yang digunakan yaitu penyuluhan dan pengukuran tingkat pengetahuan ATLM terkait manajemen laboratorium. Hasil kegiatan yaitu terdapat perbedaan hasil pre-test dan post-test yang diberikan kepada petugas ATLM. Hal ini menunjukkan bahwa penyuluhan yang dilakukan memberikan tambahan ilmu terkait manajemen laboratorium. Melalui kegiatan ini diharapkan peserta mampu mengaplikasikan manajemen mutu laboratorium dalam proses pelayanan di laboratorium klinik
An Efficient Modern Strategy to Screen Drug Candidates Targeting RdRp of SARS-CoV-2 With Potentially High Selectivity and Specificity
Authors: Zhang Haiping , Gong Xiaohua , Peng Yun , Saravanan Konda Mani , Bian Hengwei , Zhang John Z. H. , Wei Yanjie , Pan Yi , Yang Yang 
Published: July 2022

Desired drug candidates should have both a high potential binding chance and high specificity. Recently, many drug screening strategies have been developed to screen compounds with high possible binding chances or high binding affinity. However, there is still no good solution to detect whether those selected compounds possess high specificity. Here, we developed a reverse DFCNN (Dense Fully Connected Neural Network) and a reverse docking protocol to check a given compound’s ability to bind diversified targets and estimate its specificity with homemade formulas. We used the RNA-dependent RNA polymerase (RdRp) target as a proof-of-concept example to identify drug candidates with high selectivity and high specificity. We first used a previously developed hybrid screening method to find drug candidates from an 8888-size compound database. The hybrid screening method takes advantage of the deep learning-based method, traditional molecular docking, molecular dynamics simulation, and binding free energy calculated by metadynamics, which should be powerful in selecting high binding affinity candidates. Also, we integrated the reverse DFCNN and reversed docking against a diversified 102 proteins to the pipeline for assessing the specificity of those selected candidates, and finally got compounds that have both predicted selectivity and specificity. Among the eight selected candidates, Platycodin D and Tubeimoside III were confirmed to effectively inhibit SARS-CoV-2 replication in vitro with EC50 values of 619.5 and 265.5 nM, respectively. Our study discovered that Tubeimoside III could inhibit SARS-CoV-2 replication potently for the first time. Furthermore, the underlying mechanisms of Platycodin D and Tubeimoside III inhibiting SARS-CoV-2 are highly possible by blocking the RdRp cavity according to our screening procedure. In addition, the careful analysis predicted common critical residues involved in the binding with active inhibitors Platycodin D and Tubeimoside III, Azithromycin, and Pralatrexate, which hopefully promote the development of non-covalent binding inhibitors against RdRp.
Pre-existing Psychiatric Morbidity Is Strongly Associated to Takotsubo Syndrome: A Case-Control Study
Authors: Pozzi Gino , D'Amario Domenico , Princi Giuseppe , Ciliberti Giuseppe , Irano Antonella , Simone Maria Velia , Crea Filippo , Galiuto Leonarda 
Published: July 2022

BackgroundTakotsubo syndrome (TTS) is an emerging disease characterized by an acute and reversible myocardial dysfunction which may have an influence on clinical status and prognosis. Despite extensive research, its pathophysiology has not been completely elucidated; among other hypothesis, a heart-brain interaction has been proposed.MethodsThe aim of this study was to assess the impact of psychiatric disorders and of some personality types on the pathogenesis of TTS. We conducted a retrospective observational case-control study. We enrolled a total of 50 patients, 25 with a previous diagnosis of TTS and 25 patients with a history of acute coronary syndrome (ACS), that underwent a comprehensive lifetime psychiatric assessment.ResultsWe found no significant difference between TTS and ACS patients in cardiovascular risk profile. The frequency of lifetime psychiatric disorders was significantly greater in TTS. In particular, in the univariate analysis, TTS group showed a higher prevalence of mood disorders (Major Depressive Disorder, Bipolar Disorder, Dysthymia; 16 vs. 2, P &lt; 0.001) and anxiety disorder (Generalized Anxiety Disorder, Panic Disorder, Agoraphobia; 20 vs. 8, P = 0.001) compared with ACS group. There was also a significant tendency in TTS patients to psychotropic medication use, substance abuse, and psychologist or psychiatrist consulting. However, there was no difference between the groups in previous stressful events and Type D personality. Moreover, the multivariate analysis showed that mood disorders were independently associated with TTS (OR 16.9, 95% CI, 2.2–127).ConclusionOur study demonstrated that pre-existing anxiety disorders and mostly mood disorders were significantly higher in TTS patients than in ACS group, suggesting the role of psychiatric disorders as possible pathophysiological substrate of TTS.
3D Ultrasound Measurements Are Highly Sensitive to Monitor Formation and Progression of Abdominal Aortic Aneurysms in Mouse Models
Authors: Ibrahim Nahla , Bleichert Sonja , Klopf Johannes , Kurzreiter Gabriel , Knöbl Viktoria , Hayden Hubert , Busch Albert , Stiglbauer-Tscholakoff Alexander , Eilenberg Wolf , Neumayer Christoph , Bailey Marc A. , Brostjan Christine 
Published: July 2022

BackgroundAvailable mouse models for abdominal aortic aneurysms (AAAs) differ substantially in the applied triggers, associated pathomechanisms and rate of vessel expansion. While maximum aortic diameter (determined after aneurysm excision or by 2D ultrasound) is commonly applied to document aneurysm development, we evaluated the sensitivity and reproducibility of 3D ultrasound to monitor aneurysm growth in four distinct mouse models of AAA.MethodsThe models included angiotensin-II infusion in ApoE deficient mice, topical elastase application on aortas in C57BL/6J mice (with or without oral administration of β-aminoproprionitrile) and intraluminal elastase perfusion in C57BL/6J mice. AAA development was monitored using semi-automated 3D ultrasound for aortic volume calculation over 12 mm length and assessment of maximum aortic diameter.ResultsWhile the models differed substantially in the time course of aneurysm development, 3D ultrasound measurements (volume and diameter) proved highly reproducible with concordance correlation coefficients &gt; 0.93 and variations below 9% between two independent observers. Except for the elastase perfusion model where aorta expansion was lowest and best detected by diameter increase, all other models showed high sensitivity of absolute volume and diameter measurements in monitoring AAA formation and progression by 3D ultrasound. When compared to standard 2D ultrasound, the 3D derived parameters generally reached the highest effect size.ConclusionThis study has yielded novel information on the robustness and limitations of semi-automated 3D ultrasound analysis and provided the first direct comparison of aortic volume increase over time in four widely applied mouse models of AAA. While 3D ultrasound generally proved highly sensitive in detecting early AAA formation, the 3D based volume analysis was found inferior to maximum diameter assessment in the elastase perfusion model where the extent of inflicted local injury is determined by individual anatomical features.
Metformin Protects Cardiovascular Health in People With Diabetes
Authors: Chen Chong , Yuan Shiqi , Zhao Xuenuo , Qiao Mengmeng , Li Shuna , He Ningxia , Huang Liying , Lyu Jun 
Published: July 2022

BackgroundMetformin is the most commonly used drug for patients with diabetes, but there is still some controversy about whether it has a protective effect on cardiovascular health. We therefore used the National Health and Nutritional Examination Survey (NHANES) database to analyze the impact of metformin use on cardiovascular health in patients with diabetes.MethodsWe extracted the demographic data and laboratory test results of all people with diabetes in the NHANES database from January 2017 to March 2020. The outcomes were seven indicators of cardiovascular health from the American Heart Association, each was scored as 0, 1, and 2 to represent poor, moderate, and ideal health statuses, respectively. The scores for the indicators (excluding diet and glycemic status) were summed, and the sum score was then considered to indicate unhealthy (0–5) or healthy (&gt;5). Multivariate logistic regression analysis was used, and subgroup analyses were performed by age, alcohol consumption, education, and marital status.ResultsThis study included 1,356 patients with diabetes, among which 606 were taking metformin. After adjusting for all included variables, oral metformin in patients with diabetes had a protective effect on the cardiovascular health of patients (OR = 0.724, 95% CI = 0.573–0.913, P = 0.007). Subgroup analysis indicated that metformin protects the cardiovascular health of people with diabetes more clearly in those who are young (OR = 0.655, 95% CI = 0.481–0.892, P = 0.007), married (OR = 0.633, 95% CI = 0.463–0.863, P = 0.003), and drink alcohol (OR = 0.742, 95% CI = 0.581–0.946, P = 0.016).ConclusionThis study found that metformin has a protective effect on the cardiovascular health of patients with diabetes. The study findings support the general applicability of metformin.