bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pediatric lumbar disc herniation: a report of two cases and review of the literature
Authors: Wang Yi , Xu Yan , Tian Guogang , Dai Guogang 
Published: June 2022

AbstractBackgroundLumbar disc herniation (LDH) is not a common condition in children. Most reports on pediatric LDH concern the outcomes of surgeries performed in children in whom nonsurgical treatment failed while the outcome of nonsurgical treatment of LDH in children was rarely reported.Cases presentationCase 1: a 10-year-old girl presented with back pain and sciatica in her left leg for over 3 months. The physical examination revealed exacerbation of back pain by waist extension or flexion, and a positive Lasegue’s sign was revealed in her left leg. Magnetic resonance imaging (MRI) revealed lumbar disc herniation at the L5/S1 level. She was diagnosed with LDH. After receiving nonsurgical treatment of traditional Chinese medicine (TCM) for 30 days, the girl had mild low back pain and sciatica and the symptoms had resolved completely at the 3-month follow-up. There was no recurrence within the following 2 years. MRI performed 30 months later revealed that the herniated disc did not shrink significantly. However, she was totally asymptomatic at the follow-up performed 30 months later. Case 2: a 13-year-old boy presented with sciatica in his left leg for over 3 months. The physical examination revealed that Lasegue’s sign was positive in the left leg, the level of muscle strength in the left ankle plantar flexors was grade 4. MRI revealed a lumbar disc herniation at the L5/S1 level. He was diagnosed with LDH. The boy underwent 2 weeks of TCM treatment, and exhibited a favorable outcome: only mild pain was noticed in his left buttocks after walking for more than 15 min. He was asymptomatic at the 3-month follow-up and there was no recurrence within the next 3 years. MRI scan performed at 40 months later showed no significant resorption of the herniated disc. However, he was totally asymptomatic at the follow-up performed 40 months later.ConclusionsFor the nonsurgical treatment of pediatric LDH, resorption of herniated discs is not necessary for favorable long-term outcomes, and children with symptomatic LDH may become asymptomatic without resorption.
High-sensitivity C-reactive protein as a better predictor of post-thrombolytic functional outcome in patients with previous antiplatelet therapy
Authors: Li Tan , Yu Qiannan , Wang Yiqing , Cai Xiuying , Kong Yan , Zhao Hongru , Diao Shanshan , Qin Yiren , Fang Qi 
Published: June 2022

Abstract
Background
C-reactive protein (CRP) is an important biomarker of inflammation and plays a pivotal role in predicting the clinical prognosis of cardiovascular and cerebrovascular diseases. However, the mechanism of inflammation influencing the outcome of patients with ischemic stroke are unknown.

Aims
We aim to investigate the association between hsCRP and mRS in 194 eligible patients by therapy-stratified analyses.

Methods
The modification effects of antiplatelet therapy on the association between mRS and different exposure variables were analyzed. The retained variables were analyzed in the receiver operating characteristic (ROC) curve to discriminate patients with poor outcome.

Results
hsCRP was positively correlated with mRS in therapy-stratified analyses. There was a statistical modification effect of antiplatelet therapy on the association of hsCRP and mRS (P for interaction = 0.0101). The discriminative effect of poor outcome was further verified by ROC curve analyses (AUCwith from 0.758 to 0.872, AUCwithout from 0.709 to 0.713).

Conclusions
hsCRP is correlated with the clinical outcome of patients treated with IVrt-PA, and may be a better predictor of post-thrombolytic functional outcome in patients with previous antiplatelet therapy than in non-used patients.
Transfer RNA-derived small RNA: an emerging small non-coding RNA with key roles in cancer
Authors: Gu Xinliang , Zhang Yu , Qin Xinyue , Ma Shuo , Huang Yuejiao , Ju Shaoqing 
Published: June 2022

AbstractTransfer RNAs (tRNAs) promote protein translation by binding to the corresponding amino acids and transporting them to the ribosome, which is essential in protein translation. tRNA-derived small RNAs (tsRNAs) are derived fragments of tRNAs that are cleaved explicitly under certain conditions. An increasing amount of research has demonstrated that tsRNAs have biological functions rather than just being degradation products. tsRNAs can exert functions such as regulating gene expression to influence cancer progression. Their dysregulation is closely associated with various cancers and can serve as diagnostic and prognostic biomarkers for cancer. This review summarizes the generation, classification, and biological functions of tsRNAs, and highlights the roles of tsRNAs in different cancers and their applications as tumor markers.
Pembrolizumab activity in patients with Fanconi anemia repair pathway competent and deficient tumors
Authors: Villalona-Calero Miguel A. , Diaz John P. , Duan Wenrui , Diaz Zuanel , Schroeder Eric D. , Aparo Santiago , Gatcliffe Troy , Albrecht Federico , Venkatappa Siddhartha , Guardiola Victor , Garrido Sara , Rubens Muni , DeZarraga Fernando , Vuong Hao 
Published: June 2022

Abstract
Background
Given the observed antitumor activity of immune-checkpoint-inhibitors in patients with mismatch-repair deficient (MSI-H) tumors, we hypothesized that deficiency in homologous-recombination-repair (HRR) can also influence susceptibility.

Methods
Patients with disease progression on standard of care and for whom pembrolizumab had no FDA approved indication received pembrolizumab. Patients with MSI-H tumors were excluded. Objectives included immune-related objective response rate (iORR), progression-free survival (PFS) and 20-weeks-PFS. Pembrolizumab was given every 3 weeks and scans performed every six. We evaluated a triple-stain (FANCD2foci/DAPI/Ki67) functional assay of the Fanconi Anemia (FA) pathway: FATSI, in treated patients’ archived tumors. The two-stage sample size of 20/39 patients evaluated an expected iORR≥20% in the whole population vs. the null hypothesis of an iORR≤5%, based on an assumed iORR≥40% in patients with functional FA deficiency, and < 10% in patients with intact HRR. An expansion cohort of MSI stable endometrial cancer (MS-EC) followed. Exploratory stool microbiome analyses in selected patients were performed.

Results
Fifty-two patients (45F,7M;50-evaluable) were enrolled. For the 39 in the two-stage cohort, response evaluation showed 2CR,5PR,11SD,21PD (iORR-18%). FATSI tumor analyses showed 29 competent (+) and 10 deficient (−). 2PR,9SD,17PD,1NE occurred among the FATSI+ (iORR-7%) and 2CR,3PR,2SD,3PD among the FATSI(−) patients (iORR-50%). mPFS and 20w-PFS were 43 days and 21% in FATSI+, versus 202 days and 70% in FATSI(−) patients. One PR occurred in the MS-EC expansion cohort.

Conclusions
Pembrolizumab has meaningful antitumor activity in malignancies with no current FDA approved indications and FA functional deficiency. The results support further evaluation of FATSI as a discriminatory biomarker for population-selected studies.
CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in Huntington’s disease
Authors: Yu Dahyun , Zarate Nicole , White Angel , Coates De’jah , Tsai Wei , Nanclares Carmen , Cuccu Francesco , Yue Johnny S. , Brown Taylor G. , Mansky Rachel H. , Jiang Kevin , Kim Hyuck , Nichols-Meade Tessa , Larson Sarah N. , Gundry Katherine , Zhang Ying , Tomas-Zapico Cristina , Lucas Jose J. , Benneyworth Michael , Öz Gülin , Cvetanovic Marija , Araque Alfonso , Gomez-Pastor Rocio 
Published: June 2022

AbstractHuntington’s disease (HD) is a neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the HTT gene for which no therapies are available. HTT mutation causes protein misfolding and aggregation, preferentially affecting medium spiny neurons (MSNs) of the basal ganglia. Transcriptional perturbations in synaptic genes and neuroinflammation are key processes that precede MSN dysfunction and motor symptom onset. Understanding the interplay between these processes is crucial to develop effective therapeutic strategies to treat HD. We investigated the role of protein kinase CK2α’, a kinase upregulated in MSNs in HD and previously associated with Parkinson’s disease (PD), in the regulation of neuroinflammation and synaptic function in HD. We used the heterozygous knock-in zQ175 HD mouse model and compared that to zQ175 mice lacking one allele of CK2α’ (zQ175:CK2α’(±)). CK2α’ haploinsufficiency in zQ175 mice resulted in decreased levels of pro-inflammatory cytokines, HTT aggregation, astrogliosis and transcriptional alterations of synaptic genes related to glutamatergic signaling. zQ175:CK2α’(±) mice also presented increased frequency of striatal miniature excitatory postsynaptic currents (mEPSCs), an indicator of synaptic activity, and improved motor coordination compared to zQ175 mice. Neuropathological and phenotypic changes mediated by CK2α’ were connected to alpha-synuclein (α-syn) dysregulation and correlated with differences in α-syn serine 129 phosphorylation (pS129-α-syn), a post-translational modification involved in α-synucleinopathy and shown to be regulated by CK2 in PD. pS129-α-syn was increased in the nuclei of MSNs in zQ175 mice and in the striatum of patients with HD, and it decreased in zQ175:CK2α’(±) mice. Collectively, our data established a novel connection between CK2α’, neuroinflammation and synaptic gene dysregulation with synucleinopathy in HD and suggested common molecular mechanisms of neurodegeneration between HD and PD. Our results also support CK2α’ inhibition as a potential therapeutic strategy to modulate neuronal function and neuroprotection in HD.
Genome-level analysis of BpR2R3-MYB family genes transcribed in seedlings of Betula platyphylla and BpR2R3-MYB15 enhanced flavonoid production
Authors: Liu Huimin , Yu Zhongyang , Fan Guizhi , Zheng Baojiang 
Published: June 2022

Abstract
Background
Flavonoids have a wide range of biological activities in plant development, stress resistance and human health, etc. R2R3-MYBs are one of the key elements in regulation of flavonoid production, but their functional importance in Betula platyphylla remains elusive.

Methods
The full-length transcriptome data of 30-day-old seedlings of Betula platyphylla were used to identify BpR2R3-MYB family genes, and their gene structure, chromosome distribution and syntenic relationships were predicted by bioinformatics methods. Agrobacterium-mediated transient transformation was used to verify the function of BpR2R3-pMYB15 in flavonoid production.

Results
44 BpR2R3-MYB family genes expressed in seedlings of Betula platyphylla were identified and found to be unevenly distributed in 11 chromosomes. Among them, 90.90% of the BpR2R3-MYBs had introns, and only four genes had no introns. Five gene pairs with segment duplication were found, and their Ka/Ks ratios were less than 1. Thirty orthologs between Betula platyphylla and Arabidopsis thaliana and 68 orthologs between Betula platyphylla and Populus trichocarpa were detected. Five BpR2R3-MYBs were clustered with R2R3-MYB genes related to flavonoid synthesis, and BpR2R3-pMYB15 had the highest correlation coefficients between the value of gene expression and flavonoid content. BpR2R3-pMYB15 was cloned, and its transient overexpression obtained using Agrobacterium-mediated transformation positively regulated flavonoid production.

Conclusion
This work enriches the collection of R2R3-MYBs related to flavonoid production in seedlings of Betula platyphylla.

Graphical Abstract
Using ensemble quantitative precipitation forecast for rainfall-induced shallow landslide predictions
Authors: Ho Jui-Yi , Liu Che-Hsin , Chen Wei-Bo , Chang Chih-Hsin , Lee Kwan Tun 
Published: June 2022

AbstractHeavy rainfall brought by typhoons has been recognised as a major trigger of landslides in Taiwan. On average, 3.75 typhoons strike the island every year, and cause large amounts of shallow landslides and debris flow in mountainous region. Because landslide occurrence strongly corresponds to the storm dynamics, a reliable typhoon forecast is therefore essential to landslide hazard management in Taiwan. Given early warnings with sufficient lead time, rainfall-induced shallow landslide forecasting can help people prepare disaster prevention measures. To account for inherent weather uncertainties, this study adopted an ensemble forecasting model for executing precipitation forecasts, instead of using a single-model output. A shallow landslide prediction model based on the infinite slope model and TOPMODEL was developed. Considering the detailed topographic characteristics of a catchment, the proposed model can estimate the change in saturated water table during rainstorms and then link with the slope-instability analysis to clarify whether shallow landslides can occur in the catchment.Two areas vulnerable to landslide in Taiwan were collected to test the applicability of the model for landslide prediction. Hydrological data and landslide records derived from 15 typhoons events were used to verify the applicability of the model. Three indices, namely the probability of detection (POD), false alarm ratio (FAR), and threat score (TS), were used to assess the performance of the model. The results indicated that for landslide prediction through the proposed model, the POD was higher than 0.73, FAR was lower than 0.33, and TS was higher than 0.53. The proposed model has potential for application in landslide early warning systems to reduce loss of life and property.
Depth profile of frictional properties in the inner Nankai accretionary prism using cuttings from IODP Site C0002
Authors: Fujioka Riho , Katayama Ikuo , Kitamura Manami , Okuda Hanaya , Hirose Takehiro 
Published: June 2022

AbstractWe conduct frictional experiments using cuttings collected at Nankai Trough IODP Site C0002 over 980.5–3262.5 mbsf (meters below seafloor) depth interval to better understand the frictional properties through the accretionary prism. The experiments are conducted at the in situ effective normal stresses (9–37 MPa) under brine-saturated conditions, and the slip velocity is abruptly changed in a stepwise manner to either of 0.3, 3, or 33 µm/s after the steady-state friction is reached. The friction coefficient (μ) of the cuttings samples ranges from 0.45 to 0.60, with a slight increase in μ with increasing depth, related to decreasing smectite content. The velocity dependence of friction (a − b) is positive at all depths and ranges from 0.001 to 0.006, which indicates a velocity-strengthening behavior; these values are consistent with relatively homogeneous deformation microstructures. The critical slip distance (Dc) ranges from 0.5 to 123 μm, with relatively large values obtained for the smectite-rich samples. The changes in both the friction coefficient and rate- and state-friction parameters are likely associated with mineralogical change and consolidation with increasing depth. Although all of the cuttings samples collected from Site C0002 exhibit a velocity-strengthening behavior, a slight decreasing trend in a − b with increasing depth indicates either a nearly neutral velocity dependence or a possible transition to velocity-weakening behavior at greater depths, which may be attributed to the occurrence of slow earthquakes in the Nankai accretionary prism.
Seismicity distribution in the Tonankai and Nankai seismogenic zones and its spatiotemporal relationship with interplate coupling and slow earthquakes
Authors: Yamamoto Yojiro , Yada Shuichiro , Ariyoshi Keisuke , Hori Takane , Takahashi Narumi 
Published: June 2022

AbstractWe conducted seismic tomography to estimate the seismic velocity structure and to evaluate the spatiotemporal distribution of interplate earthquakes of the Kii Peninsula, central Honshu, Japan, where the Tonankai and Nankai megathrusts are located. Microearthquakes were quantitatively detected by using the data from a cable-type seafloor seismic observation network, completed in 2015. Our velocity model was consistent with the previous 2-D active-source surveys, which reported the areal extent of key structures: a high-velocity zone beneath Cape Shionomisaki, a subducted seamount off Cape Muroto, and the subducted Paleo-Zenith Ridge. The absence of any other subducted seamount with the same or larger spatial scale, than the identified key structures, was confirmed. Our velocity model also revealed that there was not a simple relationship between areas of large coseismic slip or strong interplate coupling and areas of high velocity in the overriding plate. Relocated hypocenters widely ranged from the upper plate to within the slab, while the most active region was attributed to the oceanic crust in the aftershock region of 2004 off-Kii earthquake. Compared with the results from the land-based observation network, the accuracy of the focal depth estimation was substantially improved. Furthermore, we identified the seismic activity in the vicinity of the plate boundary and determined 14 locations for interplate seismicity areas. They were primarily distributed in the range of seismogenic zone temperature (150–350 °C) along the plate boundary and were located outside of the strong interplate coupling zone. Several active areas of interplate earthquakes exhibited clustered activity during the periods of slow-slip events, observed and accompanied with shallow very-low-frequency earthquakes. Thus, regular interplate microearthquakes became active at the plate boundary in the conjunction with slow slip. In summary, as regular earthquakes provide a more accurate source location than slow earthquakes and can detect events of smaller magnitude, monitoring such interplate earthquakes may reveal spatiotemporal variations in the stick–slip conditions on the plate boundary.
Effects of Different Long-Term Exercise Modalities on Tissue Stiffness
Authors: Thomas Ewan , Ficarra Salvatore , Nakamura Masatoshi , Paoli Antonio , Bellafiore Marianna , Palma Antonio , Bianco Antonino 
Published: June 2022

AbstractStiffness is a fundamental property of living tissues, which may be modified by pathologies or traumatic events but also by nutritional, pharmacological and exercise interventions. This review aimed to understand if specific forms of exercise are able to determine specific forms of tissue stiffness adaptations. A literature search was performed on PubMed, Scopus and Web of Science databases to identify manuscripts addressing adaptations of tissue stiffness as a consequence of long-term exercise. Muscular, connective, peripheral nerve and arterial stiffness were considered for the purpose of this review. Resistance training, aerobic training, plyometric training and stretching were retrieved as exercise modalities responsible for tissue stiffness adaptations. Differences were observed related to each specific modality. When exercise was applied to pathological cohorts (i.e. tendinopathy or hypertension), stiffness changed towards a physiological condition. Exercise interventions are able to determine tissue stiffness adaptations. These should be considered for specific exercise prescriptions. Future studies should concentrate on identifying the effects of exercise on the stiffness of specific tissues in a broader spectrum of pathological populations, in which a tendency for increased stiffness is observed.
Determinants of grandparent–grandchild digital contact in Italy
Authors: Arpino Bruno , Meli Eleonora , Pasqualini Marta , Tomassini Cecilia , Cisotto Elisa 
Published: June 2022

AbstractThe broad definition of intergenerational contact includes not only meeting face-to-face, but also the important element of communication at a distance, such as via telephone. With the pervasion of the Internet and electronic devices, digital contact has become another increasingly important option. We examined digital contact between grandparents and grandchildren in comparison with traditional forms of contact (i.e. face-to-face and by telephone) using Italian data from the 2016 Families, Social Subjects and life cycle (FSS) survey. We found that grandparents belonging to younger cohorts, those with higher education, those who lived in urban areas and those in better health were more likely to maintain digital contact with their grandchildren. Results also show that digital contact tends to compensate for a lack of (frequent) face-to-face contact, and to cumulate with telephone contact. Our results have significant implications for the current and future development of intergenerational relationships as they suggest an increasing role of digital contact for distant geographical situations. Against the background of persisting inequalities in the access and the use of the Internet, our findings emphasise the need to improve digital network connectivity and user skills, particularly among specific sub-groups of the population.
Impact of active and latent concerns about COVID-19 on attention
Authors: Sisk Caitlin A. , Toh Yi Ni , Jun Jihyang , Remington Roger W. , Lee Vanessa G. 
Published: June 2022

AbstractThe interactions between emotion and attention are complex due to the multifaceted nature of attention. Adding to this complexity, the COVID-19 pandemic has altered the emotional landscape, broadly heightening health and financial concerns. Can the heightened concerns about COVID-19 impair one or more of the components of attention? To explore the connection between heightened concerns about COVID-19 and attention, in a preregistered study, we collected survey responses from 234 participants assessing levels of concerns surrounding COVID-19, followed by four psychophysics tasks hypothesized to tap into different aspects of attention: visual search, working memory, sustained attention, and cognitive control. We also measured task-unrelated thoughts. Results showed that task-unrelated thoughts, but not survey reports of concern levels, negatively correlated with sustained attention and cognitive control, while visual search and working memory remained robust to task-unrelated thoughts and survey-indicated concern levels. As a whole, these findings suggest that being concerned about COVID-19 does not interfere with cognitive function unless the concerns are active in the form of task-unrelated thoughts.