bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Mindfulness-Based Stress Reduction on Blood Pressure, Mental Health, and Quality of Life in Hypertensive Adult Women: A Randomized Clinical Trial Study
Authors: Babak Anahita , Motamedi Narges , Mousavi Seyedeh Zeinab , Darestani Nadia Ghasemi 
Published: October 2022

Background: Hypertension is one of the most important causes of cardiovascular diseases. Patients with hypertension have a lower quality of life. We aimed to evaluate the effects of mindfulness meditation on blood pressure, mental health, and quality of life in patients with hypertension.
Methods: This randomized clinical trial was performed in 2019 in Isfahan. Eighty adult women with Stage I or II hypertension were included and assigned randomly to 2 groups: 12 weeks of mindfulness-based stress reduction (MBSR) and routine care. At baseline and 1 week after the end of the intervention, blood pressure, stress, depression, anxiety, and quality of life of the studied participants were measured using the Depression, Anxiety, and Stress Scale-21 (DASS-21) and 36-Item Short Form Survey (SF-36) questionnaires. The data were analyzed using the independent t-test, the paired t-test, and the MANCOVA test.
Results: After the intervention, the mean systolic and diastolic blood pressures decreased significantly in the intervention group compared with the baseline (142.82±11.01 mmHg vs 133.7±510.43 mmHg for systolic pressure and 86.12±8.24 mmHg vs 79.15±6.26 mmHg for diastolic pressure) and the control group (140.18±14.27 mmHg vs 142.15±10.23 mmHg for systolic pressure and 84.62± 9.22 vs mmHg 88.51±8.54 mmHg for diastolic pressure; P=0.001). There was also a significant increase in quality of life, stress, anxiety, and depression scores in the intervention group (P<0.05).
Conclusion: The 12-week MBSR program resulted in a significant reduction in the mean systolic and diastolic blood pressures and improvement in mental health and different aspects of quality of life.
PENGARUH MEDIA LOOSE PART TERHADAP KEMAMPUAN PRA MENULIS ANAK PADA KELOMPOK B
Authors: Leonia Rizqy Aisyah , Handayani Tutut , Putri Yecha Febrieanitha 
Published: October 2022

Tujuan penelitian ini adalah untuk mengetahui apakah penggunaan media loose part berpengaruh terhadap kemampuan menulis anak kelompok B di Tebing Tinggi. Subjek penelitian ini adalah 20 anak yang dibagi menjadi dua kelompok yaitu kelompok eksperimen dan kelompok kontrol. Penelitian ini menggunakan metode kuantitatif melalui metode eksperimen, dan menggunakan desain eksperimen semu dan desain kelompok kontrol pre-test dan post-test. Teknik pengumpulan data yang digunakan adalah observasi, tes dan wawancara. Teknik analisis data dalam bentuk statistik non parametrik uji tingkat Kolmogrov Smirnov dengan data hasil penelitian pretest dan posttest. Hasil penelitian ini menunjukkan bahwa rata-rata kemampuan menulis anak kelas eksperimen dan kelas kontrol adalah 42,5 dan 40,7, sedangkan nilai post-test anak kelas eksperimen dan kelas kontrol rata-rata 82 dan 72,3. nilai adalah 0,000, dan kurang dari 0,05 menunjukkan efek.
SISTEM PEMBELAJARAN DI TK SATU ATAP LIMA PULUH SELAMA MASA PANDEMI
Authors: Munasti Kholida , Na'imah Na'imah 
Published: October 2022

The epidemic of coronavirus disease 19 (Covid-19) has dominated the world for about two years. This disrupts all human activity, one of which is in the field of education. Learning during a pandemic really worries teachers and parents about learning that schools provide students with an online system. Therefore, researchers want to see the online learning process during a pandemic. The purpose of this study was to find a learning system for the TK Satu Atap Lima Puluh in Kabupaten Batu Bara, Sumatera Utara during the Covid-19 pandemic. The method chosen in this study is qualitatively descriptive. Observations and interviews were used to collect data. The respondents to the survey were parents of one teacher and four students. The data analysis technique used is to describe exactly what the researcher receives. The research phase begins with observations, interviews, data reductions, and data analysis. The results showed that the learning process still followed the demands of parents and schools, the learning media did not vary much even mostly only used blackboards and markers and the teacher only explained what was written. Researchers recommend that this research be done on a larger scale so that more meaningful learning can be applied to other educational environments.
PEMANFAATAN MEDIA SOSIAL WHATSAPP SEBAGAI MEDIA PEMBELAJARAN DI MASA PANDEMI UNTUK ANAK USIA DINI
Authors: Putri Yuniar Eka , Elita Rd Funny Mustikasari , Gemiharto Ilham 
Published: October 2022

Kebijakan social distancing dan physical distancing yang ditetapkan pemerintah dalam rangka memutus penyebaran Covid-19 memberikan dampak pada kegiatan belajar mengajar di Pendidikan Anak Usia Dini (PAUD). Proses belajar mengajar pada anak usia dini yang cenderung melibatkan tatap muka, interaksi dan keterlibatan secara langsung harus dilakukan melalui pembelajaran online atau daring. Diperlukan sebuah media pembelajaran yang dapat menciptakan atmosfer sekolah walaupun kegiatan sekolah ditutup, yaitu melalui pemanfaatan media sosial WhatsApp karena dinilai sangat mudah digunakan bagi orang tua dan memiliki banyak fitur yang memudahkan kegiatan belajar mengajar bagi anak usia dini. Penelitian ini mengambil tujuan untuk melihat bagaimana dan sejauh apa pemanfaatan media sosial WhatsApp dapat mengubah perilaku belajar anak usia dini di masa pandemi. Penelitian ini menggunakan metode kualitatif deskriptif dengan pendekatan studi kasus dan mengambil lokus penelitian di TPA Bale Bermain Anwari Jakarta Timur. Hasil dari penelitian ini adalah penggunaan media sosial WhatsApp dinilai cukup efektif sebagai media pembelajaran daring karena dapat menjadi sarana edukasi dan evaluasi dari kegiatan belajar mengajar bagi anak usia dini. Namun demikian, penggunaan jaringan internet masih menjadi kendala bagi guru dan orang tua sehingga membutuhkan kebijakan baru dalam dunia pendidikan selama masa pandemi agar tetap tercipta pendidikan Indonesia yang bermutu dan berkualitas.
Alveolar-Capillary Region Exposure (ACRE) Model
Authors: Vitucci Eva CM , McCullough Shaun D. 
Published: October 2022

Abstract
This exposure model was designed as an in vitro representation of trans-alveolar effects of airway particulate matter exposures on microvascular endothelial cells in the alveolar capillary region of the lung. The alveolar epithelial cells are represented by the NCI-H441 cell line, interstitial fibroblasts are represented by the IMR90 cell line, and microvascular endothelial cells are represented by the HULEC cell line. Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use. NOTE: There is a consolidated PDF version of this protocol in the supplementary files section below.
A metabolomic protocol for investigating the gut microbiome
Authors: Han Shuo , Guiberson Emma R. , Sonnenburg Justin L. 
Published: October 2022

Abstract
A significant hurdle that has limited progress in microbiome science has been identifying and studying the diversity of metabolites produced by the gut microbes. Gut microbial metabolism produces thousands of difficult-to-identify metabolites, which present a challenge to study their roles in host biology. Over the recent years, mass spectrometry-based metabolomics has become one of the core technologies for identifying small metabolites. However, metabolomics expertise, ranging from sample preparation, instrument use, to data analysis, is often lacking in academic labs. Most targeted metabolomics methods provide high levels of sensitivity and quantification, while they are limited to a panel of predefined molecules that may not be informative to microbiome-focused studies. Here we have developed a gut microbe-focused, wide-spectrum, targeted metabolomic protocol using Liquid Chromatography-Mass Spectrometry (LC-MS) and bioinformatic analysis. This protocol enables users to carry out experiments from sample collection to data analysis, only requiring access to a LC-MS instrument, which is often available at local core facilities. By applying this protocol to samples containing human gut microbial metabolites, spanning from culture supernatant to human biospecimens, our approach enables high confidence identification of >800 metabolites. We expect this protocol will lower the barrier in tracking gut bacterial metabolism in vitro and in mammalian hosts, propelling hypothesis-driven mechanistic studies and accelerating our understanding of the gut microbiome at the chemical level.
Association of job characteristics and burnout of healthcare workers in different positions in Rural China: a cross-sectional study
Authors: Zhang Mei , Li Sangsang , Han Dan , Wu Yunyi , Zhao Jie , Liao Hui , Ma Ying , Yan Chaoyang , Wang Jing 
Published: October 2022

Abstract
Background
Health workers in rural primary care systems are at increased risk of job burnout, but their associations with different positions have received scant attention in the literature. Thus, this study aims to measure job burnout in different positions in rural China and identify the associated factors of job burnout in different positions.
Methods
A cross-sectional study was conducted in six provincial regions in China. A total of 15,925 PC providers participated in this study, and job burnout was measured using the Maslach Burnout Inventory-General Scale. Multilevel regression analyses were used in examining factors potentially associated with job burnout in different positions.
Results
Overall, more than half of providers in different positions suffered from moderate burnout. The degree of job burnout varied among different positions. Middle managers showed higher levels personal stress, and the general staff showed the lowest interpersonal and self-evaluation dimensions of burnout. Job duty, job capability, job treatment, and career advancement in different positions are potential factors affecting these results.
Conclusions
The degree of job burnout varied among different positions in health systems. Interventions aimed at improving communication skills, professional skills, and salary adjustments may be promising strategies for alleviating burnout in different positions and improving health system performance.
Does neuroprotective magnesium sulfate treatment impact short-term outcomes in preterm infants?
Authors: Bekmez Buse Ozer , Kutman Hayriye Kanmaz , Oguz Yuksel , uygur Dilek , Elbayiyev Sarkhan , CANPOLAT FUAT EMRE , Oguz Serife , Tayman Cuneyt 
Published: October 2022

Abstract
Objective: Magnesium sulfate (MgSO4) treatment is widely used for the fetal neuro-protective purpose along with the controversy concerning side effects. A scant number of studies searched the impact of different cumulative maternal doses and neonatal serum Mg levels on short-term neonatal morbidity and mortality. We opted to carry out a study to determine the impact of neonatal serum Mg levels on immediate neonatal outcomes.
Study design: This prospective observational study was conducted between 2017 and 2021. Antenatal MgSO4 was used for the neuro-protective purpose only during the study period. Inborn preterm infants delivered between 23 and 32 weeks of gestation were enrolled consecutively. Routine neonatal serum Mg sampling was performed at the 6th hour of life. A neonatal Mg concentration of 2.5 mg/dl was used to classify MgSO4 exposed patients into two groups. Another analysis was performed between babies whose mothers were exposed to MgSO4 and those without. Finally, the groups were compared regarding neonatal outcomes.
Result: Of the 584 babies, 310 received antenatal MgSO4. The birth weight was significantly lower in the MgSO4 exposed group (1113±361g vs. 1202±388g) (p=0.005). Antenatal corticosteroid usage and intrauterine growth restriction were also noted to be higher. MgSO4 group was more likely to have bronchopulmonary dysplasia, longer duration of invasive ventilation, necrotizing enterocolitis, patent ductus arteriosus, delayed full enteral nutrition, and feeding intolerance (p<0.05). MgSO4 treatment was found to be an independent risk factor for feeding intolerance (OR 2.09 95% CI 1.2-3.6, p=0.008). Furthermore Serum Mg level was significantly correlated with feeding intolerance (r =0.21, p=0.002).
Conclusion: This study highlighted the effect of MgSO4 treatment and the the potential superiority of serum Mg level as a predictor of immediate neonatal outcomes, particularly delayed full enteral nutrition and feeding intolerance. Further studies are warranted to ascertain the optimal serum Mg concentration of preterm infants in early life that provides maximum benefit with minimal side effects.
Antiviral Activities of Artemisia vulgaris L. Extract Against Herpes Simplex Virus
Authors: Wang Yifei , Xiao Ji , Liu Ping , Hu Yuze , Liu Tao , Guo Yuying , Sun Pinghua , Zheng Junxia , Ren Zhe 
Published: October 2022

Abstract
Herpes simplex virus type 1 (HSV-1) is a double-stranded DNA virus belonging to the α herpesvirus subfamily. It often leads to herpes simplex virus encephalitis (HSE), keratitis, and cold sores. Currently, acyclovir is approved by the FDA for clinical use, but with the use of this drug, HSV-1 resistance frequently becomes increasingly intense and recurrent. Therefore, it is increasingly urgent to explore the anti-HSV-1 mechanism of other nonnucleoside analogs and natural compounds.
Artemisia vulgaris L., belonging to the Compositae family, genus Artemisia, is often used as a traditional Chinese medicine as both medicine and food. Its leaves have multiple biological functions, such as anti-inflammatory, antibacterial, insecticidal, antitumor, antiviral, antioxidant and immune regulation functions. We collected and isolated a variety of A. vulgaris L. samples from Tangyin County, Henan Province and then screened the A. vulgaris L. leaf extracts for anti-HSV-1 activity. The results of the plaque reduction test showed that the crude extract of A. vulgaris L.-Fr.8.3 had anti-HSV-1 activity, and we further verified the anti-HSV-1 activity of Fr.8.3 at the DNA, RNA and protein levels. Moreover, we found that Fr.8.3 also had a broad spectrum of antiviral activity. Finally, we explored its anti-HSV-1 mechanism, and the results showed that Fr.8.3 exerted an anti-HSV-1 effect by acting directly on the virus itself. Then, the extracts were screened on HSV-1 surface glycoproteins and host cell surface receptors for potential binding ability by molecular docking, which further verified the phenotypic results. LC-MS analysis showed that 1 and 2 were the two main components of the extracts. Docking analysis suggested that compounds from extract 1 might similarly cover the binding domain between the virus and the host cells, thus interfering with virus adhesion to cell receptors, which provides new ideas and insights for clinical drug development for herpes simplex virus type 1.
Evaluation of the Star Family Doctors Training Program: A semi-control cohort study of a novel continuing medical education program for general practitioners within a compact medical consortium
Authors: Liang Ling-Bo , Li Xu , Liu Xiang-Ping , Li Cai-Zheng , Luo Dan , Liu Feng , Mao Ting-Rui , Su Qiao-Li 
Published: October 2022

Abstract
Introduction: To determine the effectiveness of the Star Family Doctors Training Program, a comprehensive continuing medical education (CME) program for general practitioners (GPs) in a compact medical consortium.
Patients and Methods: Semi-control cohort study with quantitative and qualitative analyses in primary health care institutions in Sichuan Province. The interventions were as following: (1) The Star Family Doctors Training Program is a full-time, local government allocation program certified by the Health Department of Sichuan Province, emphasizing small group learning and practice, and using standard patients and medical patient simulators; 30 participants were selected by their institutions. (2) The control group underwent a self-financed after-work CME program using conventional lectures; 50 participants were self-selected. Short-term effectiveness assessed using immediate post-training tests and self-evaluations; long-term (1 year) effectiveness evaluated using self-reported surveys.
Results: The study involved 80 GPs (28.75% men; mean age: 38.2 ± 9.2 years). The average post-training total score was higher in the STAR group than in the control group (72.83 ± 5.73 vs. 68.18 ± 7.64; p = 0.005). Compared to the controls, the STAR group trainees showed better application of the training contents (p = 0.03), more outpatients (p = 0.02) and patients who signed family-doctor contracts (p = 0.001), and increased patient satisfaction (p = 0.03). STAR-group trainees appraised the program higher and were more willing to recommend it to colleagues (90% vs. 64%, p = 0.011).
Conclusion: The Star Family Doctors Training Program achieved good responses and provides a reference for future CME programs.
Short and long-term consequences of heat exposure on mitochondrial metabolism in zebra finches (Taeniopygia guttata)
Authors: Pacheco-Fuentes Hector , Ton Riccardo , Griffith Simon C. 
Published: October 2022

Abstract
Understanding the consequences of heat exposure on mitochondrial function is crucial as mitochondria lie at the core of metabolic processes, also affecting population dynamics. In adults, mitochondrial metabolism varies with temperature but can also depend on thermal conditions experienced during development. We exposed zebra finches to two alternative heat-treatments during early development; “constant”, maintained birds at ambient 35°C from parental pair formation to fledglings’ independence, while “periodic” heated broods at 40°C, six hours daily at nestling stage. Two years later, we acclimated birds from both experiments at 25°C for 21 days, before exposing them to artificial heat (40°C, five hours daily for ten days). After both conditions we measured red blood cells’ mitochondrial metabolism using a high-resolution respirometer. We found significantly decreased mitochondrial metabolism for Routine, Oxidative Phosphorylation (OxPhos) and Electron Transport System maximum capacity (ETS) after the heat-treatments. Additionally, the birds exposed to “constant” heat in early life showed lower oxygen consumption at the proton leak (Leak) stage after the heat treatment as adults. Females showed higher mitochondrial respiration for Routine, ETS and Leak independently of treatments, while this pattern was reversed for OxPhos coupling efficiency (OxCE). Our results show that short-term acclimation involved reduced mitochondrial respiration, and that the reaction of adult birds to heat depends on the intensity, pattern and duration of temperature conditions experienced at early-life stages. Our study provides insight into the complexity underlying variation in mitochondrial metabolism and raises questions on the adaptive value of long-lasting physiological adjustments triggered by the early-life thermal environment.
C/ebpα represses the oncogenic Runx3–Myc axis in p53-deficient osteosarcoma development
Authors: Ito Kosei , Omori Keisuke , Otani Shohei 
Published: October 2022

Abstract
Osteosarcoma (OS) is characterized by TP53 mutations in humans. In mice, loss of p53 triggers OS development, and osteoprogenitor-specific p53-deleted mice are widely used to study the process of osteosarcomagenesis. However, the molecular mechanisms underlying the initiation or progression of OS following or parallel to p53 inactivation remain largely unknown. Here, we examined the role of transcription factors involved in adipogenesis (adipo-TFs) in p53-deficient OS and identified a novel tumor suppressive molecular mechanism mediated by C/ebpα. C/ebpα specifically interacts with Runx3, a p53 deficiency-dependent oncogene, and, in the same manner as p53, decreases the activity of the oncogenic axis of OS, Runx3-Myc, by inhibiting Runx3 DNA binding. The identification of a novel molecular role for C/ebpα in p53-deficient osteosarcomagenesis underscores the importance of the Runx-Myc oncogenic axis as a therapeutic target for OS.
Knowledge, Attitude and Implementation of Evidence-Based Practice among Nurses; A National Survey
Authors: Alblooshi Sumaya Mohammed , Razzak Hira Abdul , Hijji Farha H R , Wishah Moayad Ahmad Mohammad , Alkarbi Mubarkah , Harbi Alya Zaid 
Published: October 2022

Abstract
Background:Evidence-based practice has gained prominence among the nursing profession. It is gradually being acknowledged as a crucial component of enlightening the quality of healthcare and nursing care services, and in attaining excellence in patient care. EBP across UAE has been widely adopted as country strategic direction but the knowledge, attitudes, and its implementation among nurses are yet to be explored. This research aimed to explore the knowledge, attitude and implementation of evidence-based practice (EBP) among nurses working in the United Arab Emirates (UAE).
Methods: A cross-sectional survey was administered to a random sample of 1,602 nurses across UAE healthcare settings, from January to March 2022. The self-reported EBP Questionnaire developed by Upton in 2006 and EBP Implementation scale by Melnyk, Fineout-Overholt, & Mays in 2008 were used for data collection.
Results: A total of 1602 nurses (response rate: 95.9%) completed the questionnaire. The mean age was 38.7 years (age range = 20 - >60 years). Most were staff nurses 634 (39.6), and had a nursing experience of 11-15 years -452 (28.2%). Majority of the participants were female (84.1%), and Indian (55.6%). Attitudes towards EBP showed the highest mean score of 4.93±1.7 followed by knowledge (4.5±1.4) and practice (3.92±1.7). Hence, the nurses' attitudes towards EBP appeared to be positive, their implementation and knowledge were comparatively moderate. The total EBPQ score was found to be 106.3 out of 168.
Conclusion: This finding from the study can serve as a baseline assessment for future evaluations as well as it provides a foundation for the enactment of strategies to promote EBP utilization among nurses across different health care settings in United Arab Emirates. Organizations and nursing leaders need to invest in establishing academic and professional development programs on EBP. EBP education coupled with experienced mentor support holds promise for acculturating nurses’ engagement in clinical practice informed by the best available evidence.
Modelling infection risk and emergency evacuation from bioaerosol leakage around an urban vaccine factory
Authors: Liu zhijan , Hu Chenxing , Cao Hongwei , Wu Minnan , Zhang Siqi , He Junzhou , Jiang Chuan 
Published: October 2022

Abstract
Heightened interests in modelling outdoor diffusion and transmission of bioaerosols due to the prevalence of COVID-19 in urban environment led to a better knowledge of the issues concerning exposure risks and evacuation planning. In this study, the numerical method was applied in calculating the dispersion of bioaerosols. Then, the infection risk at the pedestrian level was assessed by the improved Wells-Reily equation. Finally, the Dijkstra algorithm derived from the greedy algorithm based on the was adopted to predict the evacuation path. The results show that the deposition of bioaerosols can reach 80 m on windward side wall of the high-rise buildings driven by the buoyancy force. Compared with stable thermal stratification, the infection risk of unstable thermal stratification in upstream and downstream of the research area can increase by 4.76% and 0.88%, respectively. The present work provides a promising approach of infection risk assessment and evacuation planning for the emergency response to urban bioaerosol leakage.