bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengaruh Metode Latihan Dan Tingkat Konsentrasi Terhadap Akurasi Tendangan Pinalti
Authors: Saputra Rudy 
Published: February 2022

ABSTRAKPenelitian ini bertujuan untuk mengetahui: 1) perbedaan antara pengaruh metode latihan menggunakan sasaran tetap dan menggunakan sasaran berubah terhadap akurasi tendangan pinalti, 2) perbedaan antara pengaruh tingkat konsentrasi tinggi dan konsentrasi rendah terhadap akurasi tendangan pinalti, 3) interaksi antara metode latihan dan tingkat konsentrasi terhadap akurasi tendangan pinalti.Penelitian ini menggunakanmetode eksperimen rancangan 2x2 pengambilan sampeldengan teknik purplosive samplingyaitu 24 pemain dari total populasi 33 pemain. Teknik analisis data digunakan uji anava dua jalurdan taraf signifikansi a= 0.05 , dan uji tukey. Hasil penelitian: 1) metode latihan menggunakan sasaran tetap memberikan pengaruh lebih baik terhadap akurasi tendangan pinalti dari padalatihan menggunakan sasaran berubah, 2) pemain dengan tingkat konsentrasi tinggi memberikan pengaruh lebih baik terhadap akurasi tendangan pinalti daripada pemain dengan tingkat konsentrasi rendah, 3) terdapat interaksi antara metode latihan dan tingkat konsentrasi terhadap akurasi tendangan pinalti.Kata Kunci : Metode Latihan, Konsentrasi, Akurasi Tendangan
Використання технологій доповненої реальності під час навчання шкільного курсу хімії 11 класу
Authors: Мідак Лілія , Кузишин Ольга , Базюк Лілія 
Published: February 2022

Nowadays, the drastic development of information and communication technologies gives the opportunity to update the study process in the education establishments, using various trends of the up-to-date education. The new methods of teaching the natural sciences must meet the new requirements of providing information technologies. Including the information and communication technologies within teaching chemistry allows mobilize the education process, escalate transferring of experience and knowledge, boost education and study.
Chemistry is considered to be a complicated discipline, which manipulates the definitions that cannot be understood directly, without creating specific pictures and associations in the students’ imagination. Besides, the students' effective memorization in this subject depends not only from the theory supply methods, but also from realizing the experimental part in the form of practical and laboratory experiments, which require descent theoretic training both for the students and for the teacher. That is why augmented reality is the remedy for an appropriate presentation of the study material and for its good quality visualization.
The objective of the paper is investigating the value of providing mobile app LiCo and the developed augmented reality materials during the chemistry lessons in the 11th forms.
As far as the AR technology is multifunctional, it displays the video-files, audio-files, images, 3D-models, providing it within the school chemistry course is pretty wide. The paper gives recommendations on using the AR technology for studying chemistry in the 11th form. The investigation results have shown that the databases of education establishments and specific attention should be paid on upgrading the information and digital skills of the teachers for an effective implementation of the augmented reality technology within the study process. Providing the technologies above can be used for studying different natural science disciplines, which are studied in the general education establishments (physics, biology, geography), studying which require realization of the school experiments within the nowadays programs, and requires good quality visualization.
Можливості вивчення теми «Будова атома. Періодичний закон і періодична система хімічних елементів» як засіб проєктування індивідуальної освітньої траєкторії студента під час педагогічної практики
Authors: Самойленко Павло , Семененко К. , Білоус Ольга 
Published: February 2022

The research is aimed to define peculiarities of methodological approaches to periodic law studying during school course of chemistry and creating an individual
education trajectory (IET) of a teacher trainee for its further implementation during teaching practice. The following objectives have been specified: to perform an analysis of educational programs in Chemistry from the point of a correspondent scientific approach implementation while studying of periodic law, periodic table and atom structure; ground the sequence of educational material on the topic from the point of logical, scientific and methodological approach; provide options of projecting and IET implementation during teaching practice. In the process of research, methods of analysis, synthesis, generalization, analogy as well as hypothesis and deduction have been used.
It is shown that Methods of Chemistry teaching include three approved approaches to studying periodic law, periodic table and atom structure which are historic, logical and historical and logical. However, modern educational program in Chemistry is found not to consider the above-mentioned approaches while projecting educational material on the topic «Atom structure. Periodic law and periodic table». During structuring of educational material on the topic «Atom structure. Periodic law and periodic table» the sequence of the content has been defined according to scientific, logical and methodological approach. Projecting of IET of a student while teaching practice presupposes considering a range of subjective and objective factors which stipulate the choice of scientific and methodological approach to studying the topic «Atom structure. Periodic law and periodic table». The decisive factors among those are the level of studying abilities of both – a teacher trainee and students, as well as the level of professional pedagogical motivation development of a trainee. Defined stages of IET implementation of a teacher trainee (prognostic, diagnostic, projecting, mobilizational, process and action, assessment and reflexive, correction) are discussed in the context of definite topic studying. As a research prospective, the implementation of the projected individual educational trajectories of teachers- trainees during teaching practice is planned as well as further verification of their effectiveness.
Tetanus in Poland in 2018-2019
Authors: Bogusz J , Rumik A , Augustynowicz E , Paradowska-Stankiewicz I 
Published: February 2022

<p><strong>ABSTRACT</strong><br /><strong>BACKGROUND.</strong> Tetanus is an acute disease of the nervous system caused by tetanus toxin (neurotoxin) produced by tetanus bacilli (Clostridium tetani). This disease can be prevented by vaccination. In Poland, thanks to obligatory vaccinations of children and adolescents and high vaccination coverage, the disease has been practically eliminated. Less than 20 cases are recorded each year.<br /><strong>AIM.</strong> The aim of the work is to analyze the epidemiological indicators of tetanus in Poland in 2018-2019.<br /><strong>METHODS.</strong> The analysis of the Polish tetanus epidemiological situation in 2018-2019 was based on a review of data from the bulletins “Infections diseases in Poland in 2018”, “Infections diseases in Poland in 2019” and the analysis of the population immunization was made on the basis of data from the bulletins “Protective Vaccination in Poland in 2018”, “Protective Vaccination in Poland in 2019”.<br /><strong>RESULTS.</strong> There are 17 tetanus cases registered in 2019, in 2018 8 cases. The total incidence rate was 0.044 per 100 thousand population and was higher compared to 2018 (0.021). All reports involved hospitalized individuals.<br /><strong>SUMMARY.</strong> The few cases of tetanus in Poland occur mainly in people over 60 years of age. These can be linked to the lack of tetanus booster vaccination of adults.<br /><br /><strong>STRESZCZENIE</strong><br /><strong>WSTĘP.</strong> Tężec jest ostrą chorobą układu nerwowego, spowodowaną działaniem toksyny tężcowej (neurotoksyny) wytwarzanej przez laseczki tężca (Clostridium tetani). Chorobie tej można zapobiegać przez szczepienia.<br />W Polsce dzięki obowiązkowym szczepieniom dzieci i młodzieży oraz wysokiemu stanowi zaszczepienia choroba ta została praktycznie wyeliminowana. Każdego roku rejestrowanych jest poniżej 20 zachorowań.<br /><strong>CEL PRACY.</strong> Celem pracy była ocena sytuacji epidemiologicznej tężca w Polsce w latach 2018-2019.<br /><strong>MATERIAŁ I METODY.</strong> Oceny sytuacji epidemiologicznej tężca w Polsce w latach 2018-2019 dokonano na podstawie interpretacji danych z biuletynów ,,Choroby zakaźne i zatrucia w Polsce w 2018 roku”, ,,Choroby zakaźne i zatrucia w Polsce w 2019 roku” oraz ,,Szczepienia ochronne w Polsce w 2018 roku” i ,,Szczepienia ochronne w Polsce w 2019 roku”.<br /><strong>WYNIKI.</strong> W 2019 r. w Polsce zarejestrowano 17 przypadków tężca, zaś w 2018 r. 8 przypadków. Zapadalność ogółem wyniosła w 2019 r. 0,044 na 100 tys. mieszkańców i była wyższa w porównaniu z rokiem 2018 (0,021).<br />W latach 2018-2019 wszystkie zgłoszenia dotyczyły osób hospitalizowanych. Nieliczne zachorowania na tężec w Polsce występują głównie u osób po 60 r.ż. Nie odnotowano zachorowań u osób poniżej 30 r.ż.<br /><strong>WNIOSKI.</strong> Sporadyczne zachorowania na tężec w Polsce odnotowywane są w starszych grupach wieku. Można je powiązać z brakiem szczepień przypominających przeciw tężcowi osób dorosłych.</p>
Process Engineering of Biopharmaceutical Production in Moss Bioreactors via Model-Based Description and Evaluation of Phytohormone Impact
Authors: Ruiz-Molina Natalia , Parsons Juliana , Schroeder Sina , Posten Clemens , Reski Ralf , Decker Eva L. 
Published: February 2022

The moss Physcomitrella is an interesting production host for recombinant biopharmaceuticals. Here we produced MFHR1, a synthetic complement regulator which has been proposed for the treatment of diseases associated to the complement system as part of human innate immunity. We studied the impact of different operation modes for the production process in 5 L stirred-tank photobioreactors. The total amount of recombinant protein was doubled by using fed-batch or batch compared to semi-continuous operation, although the maximum specific productivity (mg MFHR1/g FW) increased just by 35%. We proposed an unstructured kinetic model which fits accurately with the experimental data in batch and semi-continuous operation under autotrophic conditions with 2% CO2enrichment. The model is able to predict recombinant protein production, nitrate uptake and biomass growth, which is useful for process control and optimization. We investigated strategies to further increase MFHR1 production. While mixotrophic and heterotrophic conditions decreased the MFHR1-specific productivity compared to autotrophic conditions, addition of the phytohormone auxin (NAA, 10 µM) to the medium enhanced it by 470% in shaken flasks and up to 230% and 260%, in batch and fed-batch bioreactors, respectively. Supporting this finding, the auxin-synthesis inhibitor L-kynurenine (100 µM) decreased MFHR1 production significantly by 110% and 580% at day 7 and 18, respectively. Expression analysis revealed that the MFHR1 transgene, driven by the Physcomitrellaactin5(PpAct5) promoter, was upregulated 16 h after NAA addition and remained enhanced over the whole process, whereas the auxin-responsive genePpIAA1Awas upregulated within the first 2 hours, indicating that the effect of auxin onPpAct5promoter-driven expression is indirect. Furthermore, the day of NAA supplementation was crucial, leading to an up to 8-fold increase of MFHR1-specific productivity (0.82 mg MFHR1/g fresh weight, 150 mg accumulated over 7 days) compared to the productivity reported previously. Our findings are likely to be applicable to other plant-based expression systems to increase biopharmaceutical production and yields.
Galectin-9 Mediates the Therapeutic Effect of Mesenchymal Stem Cells on Experimental Endotoxemia
Authors: Zhao Yiming , Yu Dingding , Wang Hongda , Jin Wang , Li Xiang , Hu Yonghao , Qin Yafei , Kong Dejun , Li Guangming , Ellen Acheampong , Wang Hao 
Published: February 2022

Endotoxemia remains a major cause of mortality in the intensive care unit, but the therapeutic strategy is still lacking. Mesenchymal stem cell (MSC) was reported with a tissue-oriented differentiation ability and an excellent immunoregulatory capacity. However, the immunity signaling pathways that govern MSC modulation effect are not completely understood. In our current study, MSCs (2.5 × 105 /ml) were obtained and stimulated with IFN-γ (20 ng/ml) for 72 h. Gal-9 expression on MSCs was measured by ELISA, RT-PCR, flow cytometry, and immunofluorescence, respectively. Experimental endotoxemia was induced by LPS injection (10 mg/kg, i. p.) followed by the treatment with Gal-9 high-expressing MSCs, unmodified MSCs, and Gal-9 blocking MSCs. Therapeutic effects of MSCs were assessed by monitoring murine sepsis score, survival rate, splenocyte proportion rate, inflammatory mediator levels, and pathological manifestations. The results showed that Gal-9 expressed in MSCs, and this expression was increased in a dose-dependent manner after pre-stimulating with IFN-γ. Adoptive transfer of Gal-9 high-expressing MSCs into modeling mice significantly alleviated endotoxemia symptoms and multi-organ pathological damages. Splenocyte analysis indicated that Gal-9 high-expressing MSCs could promote macrophage polarization to M2-subtype and boost Treg generation. Moreover, there were also attenuated pro-inflammatory mediator expressions (TNF-α, IL-1β, IFN-γ, and iNOS), and increased anti-inflammatory mediator expressions (T-SOD and IL-35) in the sera and damaged organ homogenates. Additionally, we found a higher expression of Gal-9 in liver, lung, and kidney homogenate. Taken together, this study reveals that the optimized immunoregulatory effect of MSCs is strongly correlated with Gal-9 high expression, which provides a novel idea for the investigation of MSC immunomodulatory mechanisms and offers a potential strategy for the treatment of endotoxemia in clinical settings.
Integrated Chromatin Accessibility and Transcriptome Landscapes of 5-Fluorouracil-Resistant Colon Cancer Cells
Authors: Zhang Bishu , Lin Jiewei , Zhang Jiaqiang , Wang Xuelong , Deng Xiaxing 
Published: February 2022

Background: 5-Fluorouracil (5-FU) is one of the most effective and widely used chemotherapeutic drugs in the treatment of colon cancer, yet chemoresistance is a common feature of colon cancer treatment, resulting in poor prognosis and short survival. Dynamic reprogramming of chromatin accessibility is crucial for proper regulation of gene transcription associated with cancer drug resistance by providing the gene regulatory machinery with rapid access to the open genomic DNA.Methods: Here, we explored the global chromatin accessibility and transcription changes by the assay for transposase-accessible chromatin using sequencing (ATAC-seq) in combination with transcriptome sequencing of both parental and 5-FU-resistant HCT15 cells, followed by integrative analysis to better understand the regulatory network underlying 5-FU resistance in colon cancer cells.Results: A total of 3,175 differentially expressed mRNAs (DEGs), lncRNAs (DELs), and miRNAs (DEMs) related to 5-FU resistance were identified, including significantly upregulated IL33, H19, and miR-17-5p; the downregulated AKR1B10, LINC01012, and miR-125b-5p; and chromatin modifiers such as INO80C, HDAC6, and KDM5A. The construction of the ceRNA regulatory network revealed that H19, HOXA11-AS, and NEAT1 might function as ceRNAs associated with 5-FU resistance in HCT15 cells. Moreover, 9,868 differentially accessible regions (DARs) were obtained, which were positively (r = 0.58) correlated with their nearest DEGs and DELs. The upregulated genes related to 4,937 hyper-accessible regions were significantly enriched in signaling pathways of MAPK, FOX, and WNT, while the 4,931 hypo-accessible regions were considered to be involved in declined biosynthesis of amino acids and nucleotide sugars, signaling pathways of Notch, and HIF-1. Analyses of the DAR sequences revealed that besides the AP-1 family, the TF motifs of FOX and KLF family members were highly enriched in hyper- and hypo-accessible regions, respectively. Finally, we obtained several critical TFs and their potential targets associated with DARs and 5-FU resistance, including FOXA1 and KLF3.Conclusion: These data provided clear insights and valuable resources for an improved understanding of the non-genetic landscape of 5-FU-resistant colon cancer cells based on chromatin accessibility and transcript levels, which allowed for genome-wide detection of TF binding sites, potential cis-regulatory elements and therapeutic targets.
A DFT Mechanistic Study on Base-Catalyzed Cleavage of the β-O-4 Ether Linkage in Lignin: Implications for Selective Lignin Depolymerization
Authors: Mensah Mary , Tia Richard , Adei Evans , de Leeuw Nora H. 
Published: February 2022

The detailed mechanism of the base-catalyzed C-C and C-O bond cleavage of a model compound representing the β-O-4 linkage in lignin is elucidated using DFT calculations at the M06/6-31G* level of theory. Two types of this linkage have been studied, a C2 type which contains no γ-carbinol group and a C3 type which contains a γ-carbinol. Cleavage of the C2 substrate is seen to proceed via a 6-membered transition structure involving the cation of the base, the hydroxide ion and the α-carbon adjacent to the ether bond. The reaction with KOH has the lowest activation barrier of 6.1 kcal mol−1 with a calculated rate constant of 2.1 × 108 s−1. Cleavage of the C3 substrate is found to proceed via two pathways: an enol-formation pathway and an epoxide-formation pathway. The first path is the thermodynamically favored pathway which is similar to the pathway for the C2 substrate and is the preferred pathway for the isolation of an enol-containing monomer. The second path is the kinetically favored pathway, which proceeds via an 8-membered transition state involving a hydrogen hopping event, and is the preferred pathway for the isolation of an epoxide-containing monomer. The KOH-catalyzed reaction also has the lowest activation barrier of 10.1 kcal mol−1 along the first path and 3.9 kcal mol−1 along the second path, with calculated rate constants of 2.4 × 105s−1 and 8.6 × 109s−1 respectively. Overall, the results provide clarity on the mechanism for the base-catalyzed depolymerization of lignin to phenolic monomers. The results also suggest both NaOH and KOH to be the preferred catalysts for the cleavage of the β-O-4 linkage in lignin.
The Impact of Occupational Noise on Hypertension Risk: A Case-Control Study in Automobile Factory Personnel
Authors: Wu Xiaomei , Li Chaoxiu , Zhang Xiaohong , Song Yumeng , Zhao Dan , Lan YueYan , Zhou Bo 
Published: February 2022

BackgroundMany epidemiological studies have investigated the relationship between occupational noise and hypertension, but with conflicting findings. This study aimed to assess the relationship between occupational noise exposure and the risk of hypertension.MethodsA case-control study was conducted to explore hypertension predictors, and then sensitivity analysis was performed based on propensity score matching (PSM). Data were collected from participants' annual physical examinations and occupational noise exposure measurements. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using logistic regression analysis. A restricted cubic spline (RCS) function was used to fit the dose-effect relationship.Results500 cases and 4,356 controls were included in the study. Multivariate logistic regression showed that an increase in the level of occupational noise [range 68–102 dB(A)] of 1 dB(A), corresponded to an increase in hypertension risk of 8.3% (OR: 1.083, 95% CI: 1.058–1.109). Compared to the first quartile, the risk of hypertension in the fourth quartile was 1.742 (95% CI: 1.313–2.310). After applying PSM to minimize bias, we obtained a population of 500 cases and 1,000 controls. Noise level was significantly associated with the risk of hypertension. In addition, the RCS curve showed the risk of hypertension was relatively stable until a predicted noise level of around 80 dB(A) and then started to increase rapidly afterward (Pnonlinear = 0.002).ConclusionsOccupational noise exposure was significantly associated with hypertension risk and there was a positively correlated dose-response relationship.
A Nomogram to Predict Regrowth After Ultrasound-Guided Radiofrequency Ablation for Benign Thyroid Nodules
Authors: Yan Lin , Zhang Mingbo , Li Xinyang , Li YingYing , Luo Yukun 
Published: February 2022

ObjectiveTo develop and validate a nomogram to predict regrowth for patients with benign thyroid nodules undergoing radiofrequency ablation (RFA).MethodsA total of 200 patients with 220 benign thyroid nodules who underwent RFA were included in this respective study. After RFA, patients were followed up at 1, 3, 6, and 12 months, and every 12 months thereafter. Regrowth was defined as an increase in nodule volume 50% over the previously recorded smallest volume. A nomogram was developed based on the variables identified by multivariate logistic regression and the model performance was evaluated by discrimination(concordance index) and calibration curves.ResultsThe incidence of regrowth was 13.64% (30/220) after a mean follow-up period of 27.43 ± 17.99 months. Multivariate logistic regression revealed initial volume (OR = 1.047, 95%CI 1.020–1.075), vascularity (OR = 2.037, 95%CI 1.218–3.404), and location close to critical structure (OR = 4.713, 95%CI 1.817–12.223) were independent factors associated with regrowth. The prognostic nomogram incorporating these three factors achieved good calibration and discriminatory abilities with a concordance index of 0.779 (95%CI 0.686–0.872).ConclusionsA prognostic nomogram was successfully developed to predict nodule regrowth after RFA, which might guide physician in stratifying patients and provide precise guidance for individualized treatment protocols.
Levothyroxine Supplementation in Euthyroid Pregnant Women With Positive Autoantibodies: A Systematic Review and Meta-Analysis
Authors: Di Girolamo Raffaella , Liberati Marco , Silvi Claudia , D’Antonio Francesco 
Published: February 2022

ObjectivesTo explore the role of levothyroxine (LT4) supplementation in affecting the outcome of pregnant euthyroid women with thyroperoxidase (TPO) antibodies.MethodsMEDLINE, EMBASE, Google Scholar, and the Web of Science databases were searched. The primary outcome was pre-term birth (PTB), defined as live birth before 37 weeks of gestation; secondary outcomes were gestational hypertension, pre-eclampsia (PE), placental abruption, miscarriage, intra-uterine death (IUD), and admission to neonatal intensive care unit (NICU). All these outcomes were explored in euthyroid women with TPO antibodies receiving compared to those not receiving LT4 supplementation in pregnancy. Random-effect meta-analyses were used to analyze the data and results reported as pooled odds ratios (OR) with their 95% confidence intervals (CI).ResultsThe risk of PTB was lower in women with TPO antibodies receiving compared to those not receiving LT4 supplementation (OR of 0.60 (95% CI 0.4-0.9). However, this association came mainly from observational studies (OR: 0.29, 95% CI 0.1-0), while RCTs did not show any beneficial effect of LT4 supplementation in affecting such outcomes. Conversely, there was no difference in the risk of gestational hypertension, preeclampsia, placental abruption, miscarriage, and admission to NICU between the two groups.ConclusionsLT4 supplementation in TPO euthyroid women is not associated with a reduced risk of PTB in TPO-positive women with normal thyroid function.