bluuebunny/crossref_metadata_2025_split huggingface dataset:
SWATH-MS identification of CXCL7, LBP, TGFβ1 and PDGFRβ as novel biomarkers in human systemic mastocytosis
Authors: Graham R. L. J. , McMullen A. A. , Moore G. , Dempsey-Hibbert N. C. , Myers B. , Graham C. 
Published: March 2022

AbstractMastocytosis is a rare myeloproliferative disease, characterised by accumulation of neoplastic mast cells in one or several organs. It presents as cutaneous or systemic. Patients with advanced systemic mastocytosis have a median survival of 3.5 years. The aetiology of mastocytosis is poorly understood, patients present with a broad spectrum of varying clinical symptoms that lack specificity to point clearly to a definitive diagnosis. Discovery of novel blood borne biomarkers would provide a tractable method for rapid identification of mastocytosis and its sub-types. Moving towards this goal, we carried out a clinical biomarker study on blood from twenty individuals (systemic mastocytosis: n = 12, controls: n = 8), which were subjected to global proteome investigation using the novel technology SWATH-MS. This identified several putative biomarkers for systemic mastocytosis. Orthogonal validation of these putative biomarkers was achieved using ELISAs. Utilising this workflow, we identified and validated CXCL7, LBP, TGFβ1 and PDGF receptor-β as novel biomarkers for systemic mastocytosis. We demonstrate that CXCL7 correlates with neutrophil count offering a new insight into the increased prevalence of anaphylaxis in mastocytosis patients. Additionally, demonstrating the utility of SWATH-MS for the discovery of novel biomarkers in the systemic mastocytosis diagnostic sphere.
HIV-reservoir size is not affected either by HCV coinfection or by direct acting antivirals (DAAs) therapy
Authors: Álvarez Beatriz , Navarrete-Muñoz María A. , Briz Veronica , Olmedillas-López Susana , Nistal Sara , Cabello Alfonso , Prieto Laura , Górgolas Miguel , García-Arranz Mariano , Benito José M. , Rallón Norma 
Published: March 2022

AbstractThe role of HCV on the HIV reservoir is controversial since the reduction on HIV-DNA levels after HCV eradication with IFNα/RBV treatment seems to be the result of drugs instead of HCV clearance. We assessed whether HCV eradication can decrease HIV-DNA content in HIV/HCV-coinfected patients treated with direct-acting antivirals, DAAs (IFNα/RBV-free regimens). Cell-associated HIV-DNA was measured by ddPCR in 25 HIV-monoinfected and 25 HIV/HCV-coinfected patients. There were no differences in HIV-DNA levels between groups neither at baseline nor at 12 weeks after DAAs treatment completion. Our results indicate that HCV does not appear to influence the HIV reservoir size and suggest the lack of an anti-HIV action for DAAs.
Self-guided quantum state tomography for limited resources
Authors: Ahmad Syed Tihaam , Farooq Ahmad , Shin Hyundong 
Published: March 2022

AbstractQuantum state tomography is a process for estimating an unknown quantum state; which is innately probabilistic. The exponential growth of unknown parameters to be estimated is a fundamental difficulty in realizing quantum state tomography for higher dimensions. Iterative optimization algorithms like self-guided quantum tomography have been effective in robust and accurate ascertaining a quantum state even with exponential growth in Hilbert space. We propose a faster convergent simultaneous perturbation stochastic approximation algorithm which is more practical in a resource-deprived situation for determining the underlying quantum states by incorporating the Barzilai–Borwein two-point step size gradient method with minimal loss of accuracy.
Microwave radiation induces neuronal autophagy through miR-30a-5p/AMPKα2 signal pathway
Authors: Hao Yanhui , Li Wenchao , Wang Hui , Zhang Jing , Wang Haoyu , Dong Ji , Yao Binwei , Xu Xinping , Zhao Li , Peng Ruiyun 
Published: March 2022

Abstract
The potential health hazards of microwaves have attracted much more attention. Our previous study found that 2856 MHz microwave radiation damaged synaptic plasticity and activated autophagy in neurons. However, the mechanisms underlying microwave-induced autophagy were still unclear. In the present study, we established neuronal damage models by exposing rat hippocampal neurons and rat adrenal pheochromocytoma (PC12) cell-derived neuron-like cells to 30 mW/cm2 microwaves, which resulted in miR-30a-5p (‘miR-30a’ for short) down-regulation and autophagy activation in vivo and in vitro. Bioinformatics analysis was conducted, and Beclin1, Prkaa2, Irs1, Pik3r2, Rras2, Ddit4, Gabarapl2 and autophagy-related gene 12 (Atg12) were identified as potential downstream genes of miR-30a involved in regulating autophagy. Based on our previous findings that microwave radiation could lead to abnormal energy metabolism in neurons, Prkaa2, encoding adenosine 5′-monophosphate-activated protein kinase (AMPK) α2 (AMPKα2, an important catalytic subunit of energy sensor AMPK), was selected for further analysis. Dual-luciferase reporter assay results showed that Prkaa2 was a downstream gene of miR-30a. Moreover, microwave radiation increased the expression of AMPKα2 and the phosphorylation of AMPKα (Thr172) both in vivo and in vitro. The transfection of PC12 cells with miR-30a mimics increased miR-30a levels, reduced AMPKα2 expression, suppressed AMPKα (Thr172) phosphorylation, and inhibited autophagy occurrence in neuron-like cells. Importantly, miR-30a overexpression abolished microwave-activated autophagy and inhibited microwave-induced AMPKα2 up-regulation and AMPKα (Thr172) phosphorylation. In conclusion, microwave radiation promoted the occurrence of autophagy in neurons through the miR-30a/AMPKα2 signal pathway.
Endoscopic ultrasound-guided gastroenterostomy versus duodenal stenting for malignant gastric outlet obstruction: an international, multicenter, propensity score-matched comparison
Authors: van Wanrooij Roy L. J. , Vanella Giuseppe , Bronswijk Michiel , de Gooyer Peter , Laleman Wim , van Malenstein Hannah , Mandarino Francesco Vito , Dell’Anna Giuseppe , Fockens Paul , Arcidiacono Paolo G. , van der Merwe Schalk W. , Voermans Rogier P. 
Published: March 2022

Abstract
Background Endoscopic duodenal stenting is the current standard treatment for malignant gastric outlet obstruction (GOO) in patients with limited life expectancy. However, duodenal stenting is prone to stent dysfunction. Endoscopic ultrasound-guided gastroenterostomy (EUS-GE) is a novel technique with potentially superior stent patency. We compared clinical success, safety, and stent dysfunction of EUS-GE and duodenal stenting in patients with malignant GOO using propensity score matching.
Methods This international, multicenter, retrospective study analyzed consecutive patients undergoing EUS-GE or duodenal stenting for GOO between 2015 and 2021 in three European centers. Primary outcomes were clinical success (GOO scoring system [GOOSS] ≥ 2) and stent dysfunction (GOOSS ≤ 1 after initial clinical success). A propensity score matching (1:1) analysis was performed using age, sex, underlying disease, disease stage, ascites, and peritoneal carcinomatosis as variables.
Results 214 patients underwent EUS-GE (n = 107) or duodenal stenting (n = 107). After propensity score matching, 176 patients were matched and compared. Technical success rates for EUS-GE and duodenal stenting were 94 % (95 %CI 89 %–99 %) vs. 98 % (95 %CI 95 %–100 %), respectively (P = 0.44). Clinical success rates were 91 % (95 %CI 85 %–97 %) vs. 75 % (95 %CI 66 %–84 %; P = 0.008). Stent dysfunction occurred in 1 % (95 %CI 0–4 %) vs. 26 % (95 %CI 15 %–37 %) of patients (P < 0.001). Adverse event rate was 10 % (95 %CI 4 %–17 %) vs. 21 % (95 %CI 12 %–29 %; P = 0.09).
Conclusion EUS-GE had higher clinical success and lower stent dysfunction, with similar safety, compared with duodenal stenting, suggesting that EUS-GE may be preferred over duodenal stenting in patients with malignant GOO.
Oxidized Forms of Olive Oil Secoiridoids: Semisynthesis, Identification and Correlation with Quality Parameters
Authors: Antoniadi Lemonia , Angelis Apostolis , Stathopoulos Panagiotis , Bata Eirini-Maria , Papoutsaki Zoe , Halabalaki Maria , Skaltsounis Leandros A. 
Published: March 2022

AbstractSecoiridoids is the prominent chemical class of olive oil polar constituents and are characterized by significant biological properties. They are abundant in different chemical forms and
relatively high concentrations compared to other components, while prone to oxidation due to their chemical motif. In recent years, oxidized derivatives of secoiridoids have been reported,
either as natural constituents of olive oil or as components which are gradually formed in all stages of its production and storage. The mono-oxidized forms of oleocanthal and oleacein named
as the respective acids have been recently isolated from olive oil and unambiguously structurally characterized. Other oxidized forms of elenolic acid or more complex secoiridoids, such as
those of oleuropein and ligstroside aglycones are also sporadically mentioned in the literature. No further information is provided since they have not been isolated in pure form in order to
be accurately identified. Most of the time, they are generally referred as oxidized forms of the parent compounds and commonly identified based on mass spectrometric data. In the current
study, the semi-synthesis of the main oxidized olive oil secoiridoids, i.e., oleocanthalic acid, oleaceinic acid, EDA acid, carboxylic form of elenolic acid, carboxylic form of ligstroside
aglycon, and carboxylic form of oleuropein aglycon is described starting from the corresponding aldehydic derivatives, using SeO2/H2O2 as oxidative agents.
Furthermore, their presence in a number of Greek olive oils was investigated as well, as possible correlation thereof with quality parameters.
Endoskopieeinsatz bei Keratoprothesen mit biologischer Haptik
Authors: Korbmacher Jonas Philipp , Borrelli Maria , Geerling Gerd 
Published: March 2022

Zusammenfassung
Hintergrund Bei schwerster Erkrankung der Augenoberfläche, bei der eine perforierende Keratoplastik nicht erfolgreich ist, kann die Implantation einer Keratoprothese eine
Visusrehabilitation erreichen. Die Osteo-Odonto-Keratoprothese bietet durch die biologische Haptik eine gute Gewebe-Integration und damit eine bessere Überlebensrate und geringere
Extrusionsrate. Aufgrund der häufig vollständigen Hornhautintransparenz stellt die Abschätzung des Visuspotenzials präoperativ eine besondere Herausforderung dar. Auch während der
Implantation einer Keratoprothese oder bei notwendigen intraokularen Revisionseingriffen besteht aufgrund der kleinen zentralen Prothesenoptik und der geometrischen Bedingungen der
Prothesenhaptik ein eingeschränkter Einblick auf die periphere Netzhaut bzw. die Ziliarkörperregion und kann den Eingriff erschweren.
Methoden An 7 Augen von 6 verschiedenen Patienten (6 männlich, 2 weiblich, mittleres Alter 66 ± 9 Jahre) wurde die endoskopische Videoassistenz eingesetzt. Dies erfolgte an 3 Augen
zur Indikationsprüfung und Abschätzung des Visuspotenzials im Rahmen einer 23-G-Vitrektomie, an 2 Augen während der Implantation und an 2 Augen bei erforderlichen Revisionseingriffen
ebenfalls im Rahmen einer Vitrektomie. Es wurde ein Endoskop mit semirigider Optik und einem Bildleitsystem mit 3000 Pixeln und 70°-Blickfeld der Firma PolyDiagnost und 23-G-Trokarsysteme
verwendet.
Ergebnisse Die Endoskopie konnte bei allen untersuchten Augen erfolgreich eingesetzt werden. Nur bei 1 von 3 Augen, bei denen sie zur Abschätzung des Visuspotenzials durchgeführt
wurde, erfolgte im Weiteren die Planung der Keratoprothese, während das Visuspotenzial befundbedingt bei 2 Augen als unzureichend bewertet wurde. Die Beurteilung von Makula und Papille war
jedoch durch die Bildauflösung auf den Ausschluss grober Pathologien limitiert. Bei der Implantation konnte die korrekte Positionierung der posterioren Haptik hinter der Wirtshornhaut
visualisiert werden. Mittels endoskopieassistierter Vitrektomie konnten retroprothetische Membranen erfolgreich entfernt werden.
Schlussfolgerung Die Endoskopieassistenz bietet Vorteile in der Visualisierung sowohl bei der Indikationsstellung, während der Implantation als auch beim Management von
Komplikationen nach der Implantation einer Keratoprothese. Die Auflösung des Bildsystems erlaubte jedoch nur eine eingeschränkte Beurteilung. Auch wenn technische Weiterentwicklungen
wünschenswert sind, stellt die Endoskopie im Kontext der Keratoprothesenchirurgie schon heute eine wertvolle Ergänzung dar.
Flow Hydrogenation of 1,3,5-Trinitrobenzenes over Cu-Based Catalysts as an Efficient Approach for the Preparation of Phloroglucinol Derivatives
Authors: Nuzhdin Alexey L. , Shchurova Irina А. , Bukhtiyarova Marina V. , Bulavchenko Olga A. , Alekseyeva Natalia A. , Sysolyatin Sergey V. , Bukhtiyarova Galina A. 
Published: March 2022

AbstractAn environmentally friendly and safe synthesis of phloroglucinol and its derivatives through the flow hydrogenation of 1,3,5-trinitrobenzenes on heterogeneous copper catalysts is reported. It was found that hydrogenation of 1,3,5-trinitrobenzene, 2,4,6-trinitrotoluene, 2,4,6-trinitroxylene, and 2,4,6-trinitromesitylene in methanol over Cu–Al mixed oxides derived from layered double hydroxides led to selective formation of the corresponding triaminobenzenes, which were isolated from the reaction mixture in the form of double salts with sulfuric acid and were stable in storage. Subsequent hydrolysis in aqueous solution gave the phloroglucinol derivatives in good yields (75–82%).
Endoscopic Endonasal Approach to the Third Ventricle Using the Surgical Corridor of the Reverse Third Ventriculostomy: Anatomo-Surgical Nuances
Authors: Karadag Ali , Camlar Mahmut , Turkis Omer Furkan , Bayramli Nijat , Middlebrooks Erik H. , Tanriover Necmettin 
Published: March 2022

Abstract
Objective Surgical access to the third ventricle can be achieved through various corridors depending on the location and extent of the lesion; however, traditional transcranial approaches risk damage to multiple critical neural structures.
Methods Endonasal approach similar to corridor of the reverse third ventriculostomy (ERTV) was surgically simulated in eight cadaveric heads. Fiber dissections were additionally performed within the third ventricle along the endoscopic route. Additionally, we present a case of ERTV in a patient with craniopharyngioma extending into the third ventricle.
Results The ERTV allowed adequate intraventricular visualization along the third ventricle. The extracranial step of the surgical corridor included a bony window in the sellar floor, tuberculum sella, and the lower part of the planum sphenoidale. ERTV provided an intraventricular surgical field along the foramen of Monro to expose an area bordered by the fornix anteriorly, thalamus laterally, anterior commissure anterior superiorly, posterior commissure, habenula and pineal gland posteriorly, and aqueduct of Sylvius centered posterior inferiorly.
Conclusion The third ventricle can safely be accessed through ERTV either above or below the pituitary gland. ERTV provides a wide exposure of the third ventricle through the tuber cinereum and offers access to the anterior part as far as the anterior commissure and precommissural part of fornix and the whole length of the posterior part. Endoscopic ERTV may be a suitable alternative to transcranial approaches to access the third ventricle in selected patients.
Tethered Rh(III)-N-(p-Tolylsulfonyl)-1,2-Diphenylethylene-1,2-Diamine Complexes: Efficient Catalysts for Asymmetric Transfer Hydrogenation
Authors: Phansavath Phannarath , Ratovelomanana-Vidal Virginie , Echeverria Pierre-Georges , Zheng Long-Sheng , Llopis Quentin , He Bin , Westermeyer Anne , Molina Betancourt Ricardo 
Published: March 2022

Abstract
Experience Delivering Tele-practice Services among Upcoming and Working Professionals of Speech Language Pathology
Authors: Bhattarai Biraj , Sanghavi Tanvi , Panchakshari Abhishek Buddiguppe 
Published: March 2022

Abstract
Introduction Since the outbreak of the COVID-19 epidemic, tele-practice has become the new normal in the field of Speech-Language Pathology. Students and professionals throughout the country are embracing this new normal. Both groups confront problems; documentation of the problems may provide insight into how to improve tele-practice services.
Aim The purpose of this study was to compare the problems that undergraduate students, postgraduate students, and working professionals in the field of Speech-Language Pathology experience in daily practice.
Methods A descriptive cross-sectional survey study using a web-based questionnaire was conducted to examine the obstacles experienced by students and working professionals in the field of speech-language pathology, as well as their perspectives about tele-practice. There was a total of 20 questions. Divided into four sections, closed questions and Likert scale questions were used for demographic details, practice aspects, experience during telerehabilitation, and evaluation and treatment.
Results The study had 118 participants (47% postgraduate students, 29% undergraduate students, and 24% professionals). Only 16% of clinicians were properly trained to provide services via tele-mode. All participants reported providing service to the pediatric population to be challenging, with autism spectrum disorder, fluency disorders, and hearing impairment to be difficult to handle cases. In tele-mode, undergraduate students reported a decrease in the number of cases and difficulties selecting therapy materials when compared with the other two groups. All three groups reported a lack of evidence-based teletherapy resources available. There was no difference in perceived difficulty between the three groups while conducting assessment and achieving treatment goals via tele-mode.
Conclusion Tele-practice is generally recognized and employed, according to data availability. In terms of resource selection, evaluation, and treatment sessions, the difficulties faced by students and experts are disturbing. In the realm of speech-language pathology, strategies to address these issues could pave the way for tele-practice. As a result, better policies are strongly advised to address these difficulties to improve future tele-mode service quality.
Temperature dependence of thermodynamic, dynamical, and dielectric properties of water models
Authors: Morozova Tatiana I. , García Nicolás A. , Barrat Jean-Louis 
Published: March 2022

We investigate the temperature dependence of thermodynamic (density and isobaric heat capacity), dynamical (self-diffusion coefficient and shear viscosity), and dielectric properties of several water models, such as the commonly employed TIP3P water model, the well-established four-point water model TIP4P-2005, and the recently developed four-point water model TIP4P-D. We focus on the temperature range of interest for the field of computational biophysics and soft matter (280–350 K). The four-point water models lead to a spectacularly improved agreement with experimental data, strongly suggesting that the use of more modern parameterizations should be favored compared to the more traditional TIP3P for modeling temperature-dependent phenomena in biomolecular systems.
Vibrational and vibronic coherences in the energy transfer process of light-harvesting complex II revealed by two-dimensional electronic spectroscopy
Authors: Zhu Ruidan , Ruan Meixia , Li Hao , Leng Xuan , Zou Jiading , Wang Jiayu , Chen Hailong , Wang Zhuan , Weng Yuxiang 
Published: March 2022

The presence of quantum coherence in light-harvesting complex II (LHCII) as a mechanism to understand the efficiency of the light-harvesting function in natural photosynthetic systems is still debated due to its structural complexity and weak-amplitude coherent oscillations. Here, we revisit the coherent dynamics and clarify different types of coherences in the energy transfer processes of LHCII using a joint method of the high-S/N transient grating and two-dimensional electronic spectroscopy. We find that the electronic coherence decays completely within 50 fs at room temperature. The vibrational coherences of chlorophyll a dominate over oscillations within 1 ps, whereas a low-frequency mode of 340 cm−1 with a vibronic mixing character may participate in vibrationally assisted energy transfer between chlorophylls a. Our results may suggest that vibronic mixing is relevant for rapid energy transfer processes among chlorophylls in LHCII.
Ab initio investigation of the role of the d-states on the adsorption and activation properties of CO2 on 3d, 4d, and 5d transition-metal clusters
Authors: Collacique Matheus N. , Ocampo-Restrepo Vivianne K. , Da Silva Juarez L. F. 
Published: March 2022

We report a theoretical investigation of the adsorption and activation properties of CO2 on eight-atom 3d, 4d, and 5d transition-metal (TM) clusters based on density functional theory calculations. From our results and analyses, in the lowest energy configurations, CO2 binds via a chemisorption mechanism on Fe, Co, Ni, Ru, Rh, Pd, Os, Ir, and Pt (adsorption energy from −0.49 eV on Pt up to −1.40 eV on Os), where CO2 breaks its linearity and adopts an angular configuration due to the charge transfer from the clusters toward the C atom in the adsorbed CO2. In contrast, it binds via physisorption on Cu, Ag, and Au and maintains its linearity due to a negligible charge transfer toward CO2 and has a small adsorption energy (from −0.17 eV on Cu up to −0.18 eV on Ag). There is an energetic preference for twofold bridge TM sites, which favors binding of C with two TM atoms, which enhances the charge transfer ten times than on the top TM sites (onefold). We identified that the strength of the CO2–TM8 interaction increases when the energy values of the highest occupied molecular orbital (HOMO) of the TM8 are closer to the energy values of the lowest unoccupied molecular orbital of CO2, which contributes to maximize the charge transfer toward the molecule. Beyond the energy position of the HOMO states, the delocalization of 5d orbitals plays an important role in the adsorption strength in TM, especially for the iron group, e.g., the adsorption energies are −1.08 eV (Fe, 3d), −1.19 eV (Ru, 4d), and −1.40 eV (Os, 5d).
Formation of clustered DNA damage in vivo upon irradiation with ionizing radiation: Visualization and analysis with atomic force microscopy
Authors: Nakano Toshiaki , Akamatsu Ken , Tsuda Masataka , Tujimoto Ayane , Hirayama Ryoichi , Hiromoto Takeshi , Tamada Taro , Ide Hiroshi , Shikazono Naoya 
Published: March 2022

Significance
DNA damage causes loss of or alterations in genetic information, resulting in cell death or mutations. Ionizing radiations produce local, multiple DNA damage sites called clustered DNA damage. In this study, a complete protocol was established to analyze the damage complexity of clustered DNA damage, wherein damage-containing genomic DNA fragments were selectively concentrated via pulldown, and clustered DNA damage was visualized by atomic force microscopy. It was found that X-rays and Fe ion beams caused clustered DNA damage. Fe ion beams also produced clustered DNA damage with high complexity. Fe ion beam–induced complex DNA double-strand breaks (DSBs) containing one or more base lesion(s) near the DSB end were refractory to repair, implying their lethal effects.