bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brain proton magnetic resonance spectroscopy in patients with Parkinson’s disease
Authors: Shoeibi Ali , Verdipour Mahdieh , Hoseini Alireza , Moshfegh Mehdi , Olfati Nahid , Layegh Parvaneh , Dadgar-Moghadam Maliheh , Farzadfard Mohammad Taghi , Rezaeitalab Fariborz , Borji Nahid 
Published: November 2022

Background: The accuracy of current laboratory and imaging studies for diagnosis and monitoring of Parkinson’s disease (PD) severity is low and diagnosis is mainly dependent on clinical examination. Proton magnetic resonance spectroscopy (MRS) is a non-invasive technique that can assess the chemical profile of the brain. In this study, we evaluated the utility of proton MRS in diagnosis of PD and determination of its severity.
Methods: Patients with PD and healthy age-matched controls were studied using proton MRS. The level of N-acetylaspartate (NAA), total creatine (Cr), and total choline (Cho), and their ratios were calculated in substantia nigra (SN), putamen (Pu), and motor cortex. PD severity was assessed by the Unified Parkinson’s Disease Rating Scale (UPDRS) and the Hoehn and Yahr scale.
Results: Compared to 25 healthy controls (18 men, age: 59.00 ± 8.39 years), our 30 patients with PD (24 men, age: 63.80 ± 12.00 years, 29 under treatment) showed no significant difference in the metabolite ratios in SN, Pu, and motor cortex. Nigral level of NAA/Cr was significantly correlated with total UPDRS score in patients with PD (r = -0.35, P = 0.08). Moreover, patients with PD with Hoehn and Yahr scale score ≥ 2 had a lower NAA/Cr level in SN compared to patients with a lower stage.
Conclusion: This study shows that 1.5 tesla proton MRS is unable to detect metabolite abnormalities in patients with PD who are under treatment. However, the NAA/Cr ratio in the SN might be a useful imaging biomarker for evaluation of disease severity in these patients.
Socioeconomic Status as Predictors of Adherence to COVID-19 Protocols by Market Men and Women in Ogun State, Nigeria
Authors: Adesina Okueso Samuel , Buraimo Olayinka , Olugbemiga Adeleye Adewale , Adekoya Folajimi Adebolu 
Published: November 2022

Background: Adherence to COVID-19 protocols is a serious issue in Nigeria and this has been worrisome to public health practitioners, including health educators. Obeying precautionary measures has been a great challenge especially among market men and women, including their customers, which appears to be due to several variables. This study examined the impact of socioeconomic status, including income level, level of education, religious affiliation, gender, family structure, cultural background, and age group of market men and women on adherence to COVID-19 protocols in Ogun State, Nigeria.
Methods: The study adopted descriptive survey research design where structured and validated questionnaire was used as the instrument for data collection. Multi-stage sampling procedure was employed to select the sample for the study from the old Ijebu and Egba components of the State, including Ita-Osu new market in Ijebu-Ode and Oba Lipede Market in Abeokuta were purposively selected for the study. In each of the market, n=250 market men and women were randomly selected using simple random sampling technique to make a total sample of n=500, out of this, n=481 questionnaires were retrieved to make a total of 96.0% rate of return.
Results: The results revealed that socioeconomic factors, including gender, income level, type of business, and family structure significantly predicted adherence to COVID-19 protocols at P< 0.05.
Conclusion: It was concluded that market men and women in Ogun State do not significantly adhere to COVID-19 protocols, and the identified socioeconomic factors significantly predicted adherence to the protocols. Recommendation was made to involve health educators at all levels to disseminate health information to promote improved health literacy and carry out interventions considering the aforementioned socioeconomic variables towards improving adherence to the protocols.
GPU Based Similarity Metrics Computation and Machine Learning Approaches for String Similarity Evaluation in Large Datasets
Authors: Baloi Aurel , Belean Bogdan , Turcu Flaviu , Peptenatu Daniel 
Published: November 2022

Abstract
The digital era brings up on one hand massive amounts of available data, and on the other hand the need of parallel computing architectures for efficient data processing. String similarity evaluation is a processing task applied on large data volumes, commonly performed by various applications such as search engines, bio-medical data analysis and even software tools for defending against viruses, spyware, or spam. String similarities are also evaluated in musical industry for matching playlist records with repertory records composed of song titles, performer artists and producers names, aiming to assure copyright protection of massmedia broadcast materials. Thus, the present paper proposes a GPU based approach for parallel implementation of the Jaro-Winkler string similarity metric computation. Further on, a thresholding-based algorithm is also implemented using GPU for matching records over large datasets. The global GPU RAM memory is used to store multiple string lines as raw data. In the case of a single string, its comparisons with the raw data are performed using the maximum number of available GPU threads and the stride operations. Moreover, based on the computed similarity metrics, an adaptive neural network approach guided by a novelty detection classifier together with a naive neural network implementation are proposed to increase the accuracy of the records matching procedure. Timing considerations and the computational complexity are detailed for the proposed approaches compared with state-of-the-art CPU and GPU approaches. A speed-up factor of 21.6 was obtained for the GPU based JaroWinkler implementation compared with the general purpose processor one, whereas improved accuracy for the records matching procedure was delivered using machine learning approaches.
The Cross-talk, Genetic Alteration, Molecular Functions, and Cluster Relevance of the Major Types of Regulated Cell Death in Bladder Cancer
Authors: Zhang Zhihong , Li Jingxian , Qi Yuanjiong , Liu Yang , Liu Xun , Li Shuai , Shi Jianxi , Gao Chao , Li Bo , Zhang Changwen , Du E 
Published: November 2022

Abstract
The dysfunction of regulated cell death (RCD) could cause human diseases such as cancer, neurodegeneration, autoimmune diseases, and infectious diseases. Recent studies have indicated that RCD has closely functional interaction. Our study aimed to explore the cross-talk, genetic alteration, functions, mechanism, and cluster relevance of the several major types of RCD, including apoptosis, ferroptosis, pyroptosis, necroptosis, autophagy-dependent cell death, in bladder cancer (BLCA). Through analyzing the public database, we found that the genes representing these five RCD types showed complex cross-talk in mRNA, protein levels, somatic mutation, and DNA copy number in BLCA. The mRNA expression of regulated cell death genes (RCDGs) was affected by DNA copy number, somatic mutation, DNA methylation, and RNA modification. Several RCDGs were essential for cell viability, and the expression of RCDGs was correlated with radiotherapeutic sensitivity and IC50 of chemotherapy drugs. Based on the expression of RCDGs, the BLCA patients were divided into two clusters. We found multiple immune-related pathways significantly enriched in cluster1/cluster2. In addition, the patients' prognosis, immune therapy response, and drug sensitivity in these two clusters were significantly different. Through the comparison between the RCD clusters and consensus cluster, we found that RCD cluster1 mainly correlated with the basal squamous cluster, and the RCD cluster2 mainly correlated with the luminal cluster. Through the weighted gene co-expression network analysis (WGCNA), we identified the high correlation RCDGs with cluster1/cluster2. We found that most cluster1-RCDGs were correlated with poor prognosis for patients, and most prognosis-protection genes correlated with cluster2. Besides, the expression of these prognosis-related RCDGs showed different distribution patterns in basal/luminal subtypes. In summary, our study conducted a comprehensive analysis of RCDGs in BLCA, which provided the foundation for subsequent experiments and therapeutic options.
Experience-dependent phosphoproteomic changes in hippocampus and neocortex correlate with the abundance of spindle oscillations during the transition between SWS and REM sleep
Authors: Souza Annie C. , Golbert Daiane C. F. , Cassoli Juliana S. , Sánchez-Gendriz Ignacio , Lima Vinícius V. F. , Cini Felipe A. , Martins-de-Souza Daniel , Ribeiro Sidarta 
Published: November 2022

Abstract
The role of sleep on memory consolidation is thought to involve experience-dependent changes in spindle oscillations and protein phosphorylation, but how these phenomena are related remains poorly understood. To gain insight into this relationship, we used electrophysiological recordings and quantitative phosphoproteomic analysis to assess spindle oscillations and phosphoprotein levels in the hippocampus (HP) and primary somatosensory cortex (S1) of adult male rats recorded across the sleep cycle. Animals were surgically implanted with multielectrode probes and after recovery were exposed or unexposed to novel objects (+ and – groups, respectively). HP and S1 samples were obtained after periods rich in either slow-wave sleep (SWS) or rapid-eye-movement sleep. Bottom-up shotgun mass spectrometry in a two-dimensional liquid chromatography-tandem mass spectrometry setup (MSE mode with label-free quantification) showed that the proteomes differed in the numbers of phosphoproteins identified by phosphoryl modification STY tags, with a total of 337 validated phosphoproteins identified in S1 and 198 in the HP. A comparison of the phosphoproteomic profiles of the treatments and regions (SWS+ versus SWS-, REM+ versus REM-, REM+ versus SWS+ and REM- versus SWS-), using clustering analysis of the significantly identified phosphoproteins, found that 51 phosphoproteins from S1 were sufficient to separate the four experimental conditions, while 37 phosphoproteins from the HP could only partially separate the groups. Fold change analysis identified 90 significantly modulated phosphoproteins related to synaptic function, actin-microtubule regulation, DNA-RNA binding, proteases-phosphatases-kinases and other regulatory functions, including CaMKII and MAPK. In both the HP and S1, nearly one third of the clustering-relevant phosphoproteins had levels significantly correlated with the abundance of spindle oscillations pooled across the transition from SWS to REM. In S1, phosphorylated Reelin was upregulated during REM compared to SWS, in proportion to the number of spindle oscillations during the transition from SWS to REM. In the HP, a voltage-gated calcium channel subunit (Cacna2d1) was down-regulated during REM+ compared to SWS+, in proportion to spindle counts. Since spindles facilitate calcium entry through the opening of voltage-dependent calcium channels, Cacna2d1 down-regulation may lead to a hippocampus-specific, REM-dependent downregulation of synaptic plasticity after exposure to novel objects. The results point to major experience-dependent differences between HP and S1 in phosphoproteomic regulation across the sleep cycle, with potential implications for memory corticalization.
Risk Assessment of Human Monkeypox Infections for Vaccine Prioritization
Authors: Zucker Roy , Lavie Gil , Sagy Yael Wolff , Arieh Noa Gur , Markovits Hila , Abu-Ahmad Weissam , Battat Erez , Ramot Noga , Beckenstein Tanya , Carmeli Guy , Mark-Amir Avner , Wagner-Kolasko Gal , Edry Amos , Duskin-Bitan Hadar , Yaron Shlomit , Peretz Alon , Netzer Doron , Arbel Ronen 
Published: November 2022

Abstract
Background
The global supply of vaccines against monkeypox virus infection (MPXVi) is significantly lower than demand. Evidence-based vaccine prioritization criteria, based on risk assessment, are essential for fair vaccine distribution. Our objective was to identify the characteristics of individuals at the highest risk for MPXVi.
Methods
This population-based cohort study included all Clalit Health Services (CHS) subjects assumed to be at risk for MPXVi, based on known characteristics of infected subjects worldwide and insights of LGBTQ+ -specialized CHS clinicians. Cox hazards models were used to identify risk factors for MPXVi in the study cohort. The study commenced on June 6, 2022, the date of the first known MPXVi in CHS patients, until July 31, 2022, when the MPXV vaccination campaign started.
Results
8,088 individuals (0.18% of the CHS total population) were identified according to the study inclusion criteria. Of those, 69 (0.85%) were infected during the study period. Risk factors for MPXVi were birth in 1980 or later, history of syphilis, HIV-PrEP use, PDE5 inhibitors use, recent STIs, and registeration to primary healthcare clinics in the Tel-Aviv district. No infections were observed in individuals with none of the factors. Individuals with 3+ risk factors had a 20-fold higher risk for MPXVi compared to those with 0-2, with 85.5% sensitivity and 77.8% specificity.
Conclusions
Counting the number of validated risk factors per individual seems to provide a robust, simple risk score for MPXVi. Weighting individuals’ risk levels against vaccine availability can assist health systems in the equitable prioritization of vaccine allocation.
Large and fast excitatory transcriptomic neuron types in human cortex preferentially express genes of intelligence and HARs.
Authors: Driessens Stan , Driessens Stan , Heyer Djai , Pieterse Isabel , Wilbers René , Mertens Eline , Waleboer Femke , Heistek Tim , Coenen Loet , Idema Sander , Hamer Philip de Witt , Noske David , de Kock Christiaan , Lee Brian , Smith Kimberly , Ting Jonathan , Lein Ed S. , Mansvelder Huibert , Goriounova Natalia 
Published: November 2022

Abstract
Human intelligence is a highly heritable trait, and GWAS have yielded numerous associated genes. These genes overlap with human accelerated regions (HARs) implicated in human brain evolution and might act on the same biological processes. Here, we investigated whether genes associated with human cognition and HAR genes are similarly expressed in adult human cortical neurons and brain areas of cognition, and how their expression relates to neuronal function and structure. We find that these gene sets are preferentially expressed in L3 excitatory neurons in middle temporal gyrus (MTG). Furthermore, neurons with higher expression had larger dendrites and faster action potential kinetics, properties previously linked to intelligence. Finally, we identify a subset of genes associated with dendritic length, with predominantly synaptic functions and high abundance of HARs. Our results indicate that mechanisms underlying human brain evolution and interindividual differences in intelligence might share genetic origin and manifest in specific neuronal types.
The experience of being a caregiver of patients with digestive cancer, from patients and caregivers' perception: a mixed study.
Authors: GRIVEL Charlotte , NIZET Pierre , MARTIN Manon , PECOUT Solange , LEPEINTRE Aurélie , TOUCHEFEU Yann , PROT-LABARTHE Sonia , EVIN Adrien , HUON Jean François 
Published: November 2022

Abstract
Objective
To explore the experience of caregivers of patients with digestive cancer and to compare the perspectives of patients and caregivers.
Methods
Caregiver-patient dyads were enrolled in this mixed-methods study. The Caregiver Reaction Assessment (CRA) and the Supportive Care Needs Survey for Partners and Caregivers (SCNS-PC) questionnaires were distributed to caregivers. Semi-structured interviews with the dyads were conducted.
Results
Thirty-two caregivers completed the questionnaires. Responses showed high self-esteem, schedule burden, and a need for care and information services. Ten dyads participated in the interviews. Three themes emerged from the caregiver’s interviews: illness is an upheaval; loneliness and helplessness are experienced; caring is a natural role with positive outcomes. Four themes emerged from patient's interviews: the caregiver naturally assumes the role and gets closer; he is the patient's anchor; his life is disrupted; anxiety and guilt accompany the desire to protect him. In comparing patient and caregiver data, we identified many similarities. The main theme of disagreement was their relationship.
Conclusions
Caregiver care does not appear to be optimal, particularly in terms of their need for information. Even though the patients have a fairly good representation of their caregiver’s experience, their opinion and their communication still need to be considered.
Outcomes after first IVF/ICSI attempts (fresh and frozen) differ between overweight and obese patients
Authors: Pretalli Jean-Baptiste , Pithois Alixane , Tio Grégory , Frontczak-Franck Sophie , Briet Maïté , Mauppin Camille , Roux Christophe 
Published: November 2022

Abstract
Background
Women with high body mass index (BMI) may have reduced natural fecundity and compromised In vitro fertilization outcomes. However, data are discordant.. Our objective was to evaluate the relationship between BMI and outcomes of IVF and/or ICSI, for the first fresh embryo transfers, and for complete (fresh and frozen) cycles.
Methods
We included 1912 consecutive cycles; 1286 (67.3%) first fresh embryo transfers and 626 (32.7%) frozen embryo transfers, all performed between 01/01/2013 and 12/31/2018 in the Department of Reproductive Medicine and Biology - Cryobiology of the University Hospital of Besançon (France). The study population comprised infertile women undergoing IVF/ICSI treatment divided into 3 groups :obese, overweight and normal BMI.
Results
The cumulative live birth rate (after fresh and frozen cycles) was lower in overweight women compared to those with normal BMI (respectively 110/421 (26.1%) vs 406/1273 (31.9%), p = 0.025), while there was no significant difference between obese (58/214 (27.1%)) and normal BMI women. The cumulative clinical pregnancy rate was lower for overweight women compared to normal BMI (respectively 128/421 (30.4%) and 467/1277 (36.6%), p = 0.021), but did not differ between obese (66/214 (30.8%)) and normal BMI women.
Conclusion
Our results raise questions about the optimal management of women undergoing IVF/ICSI. They highlight complexity of management for women with high BMI. Further research is warranted to clarify the exact contribution of overweight and obesity to outcomes. Pending further research, we might consider overweight to be as impairing as obesity in this setting.
Label-free adaptive optics single-molecule localization microscopy for whole animals
Authors: Choi Wonshik , Park Sanghyeon , Jo Yonghyeon , Kang Minsu , Hong Jin Hee , Ko Sangyoon , Kim Suhyun , Park Sangjun , Park Hae-Chul , Shim Sang-Hee 
Published: November 2022

Abstract
The specimen-induced aberration has been a major factor limiting the imaging depth of single-molecule localization microscopy (SMLM). Here, we report the application of label-free wavefront sensing adaptive optics to SMLM for deep-tissue super-resolution imaging. The proposed system measures complex tissue aberrations from intrinsic reflectance rather than fluorescence emission and physically corrects the wavefront distortion more than three-fold stronger than the previous limit. This enables us to resolve sub-diffraction morphologies of cilia and oligodendrocytes in whole intact zebrafish as well as dendritic spines in thick mouse brain tissues at the depth of up to 102 µm with localization number enhancement by up to 37 times and localization precision comparable to aberration-free samples. The proposed approach can expand the application range of SMLM to intact animals that cause the loss of localization points owing to severe tissue aberrations.
Renewable and non-renewable energy consumption, economic growth and Ecological footprint relationship in the top Russian energy importers: A panel data analysis
Authors: Öncel Abidin , Kabasakal Ali , Kutlar Aziz , Acar Samet 
Published: November 2022

Abstract
This study examines the relationship between renewable and non-renewable energy consumption, economic growth, and EF for the top 14 Russian energy importing countries in the 2000-2018 period. Through PVAR analysis, this study calculates the long-term correlation between variables using a VEC model. The VAR approach addresses the endogeneity issue by including endogenous interactions between the variables in the model. Additionally, the impulse response functions and the influence of various variables on certain lags are evaluated. Cointegration between variables was evaluated using DOLS and FMOLS panel analysis to evaluate the long-term link further. Our findings demonstrate that there is a cointegration relationship between all series. According to the impulse response analysis results, an energy shock from Russia will negatively affect the countries' economic growth and decrease the EF. Increasing both PEC and REC domestically increases EF, but the effect of REC on EF seems to be a little less. According to DOLS findings, a 10 percent increase in LNFUELS increases the elasticity of LNPGDP by 0.61%.In contrast, FMOLS findings indicate a 10% increase in the EF or LNFUELS, increasing the elasticity of LNPGDP by 1%. Although the direction of the relationship is positive when EF is considered the dependent variable, while the PEC elasticity coefficient was 0.030 in the DOLS model, it was found to be 0.023 in the FMOLS model. The elasticity value of the LNPGDP variable was found to be 0.69 in the FMOLS model.
JEL CODES: O13; O44; C59, Q56
Chronological measurement of effector CD8 T cell activity using a bioluminescence-based cell viability assay
Authors: Hayashi Ryota , Nakatani Hayato , Kawahata Hinami , Fujie Ryonosuke , Kurowarabe Kaoru , Hayasaka Haruko 
Published: November 2022

Abstract
Background
Although cell-mediated cytotoxicity has been evaluated with various protocols, methods for monitoring cytotoxicity in a time series have not been established. This work describes a method for evaluating cytotoxicity using a real-time luminometer.
Materials & Methods
The efficiency of effector CD8 T cell expansion from melanoma-bearing splenocytes was analyzed. The effect of CD8 T cells on the viability of luciferase-expressing target cells was measured by bioluminescence.
Results
Melanoma-specific effector CD8 T cells were differentiated by in vitro coculture. The melanoma cell growth was significantly inhibited in the presence of in vitro expanded activated T cells inthe bioluminescence-basedtime-lapse analysis.
Conclusion
The bioluminescence-based assay is a useful method for monitoring the time course of cell viability of target tumor cells.