bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel stable biomimetic adhesive coating for functionalization of orthodontic brackets against bacterial colonization and white spot lesions
Authors: Singer Lamia , Karačić Sabina , Bierbaum Gabriele , Palmer Brianne , Kirschneck Christian , Bourauel Christoph 
Published: January 2025

Abstract
Background
This study aimed to evaluate the efficacy of polydopamine (PDA) functionalization on orthodontic brackets in inhibiting biofilm formation and promoting surface bioactivity to buffer the acidity of caries-causing bacteria around orthodontic brackets and prevent demineralization. The stability of the coating in artificial saliva (AS) and distilled water was evaluated, along with its effect on pH changes in simulated body fluid (SBF) and distilled water.

Methods
Maxillary incisor orthodontic brackets underwent PDA functionalization using a dopamine hydrochloride solution following a specific protocol. Biofilm formation on both control (Br-0) and coated (Br-PDA) brackets was assessed immediately after coating and after two months of aging (Aged Br-PDA) in artificial saliva. The adherent biofilm bacteria on brackets were quantified with colony count assessment and optical density. Surface morphology, Bioactivity, and coating stability were analyzed using Scanning Electron Microscopy (SEM). Coated and uncoated samples were immersed in SBF and deionized water, and pH changes were monitored over 7 days using a pH meter.

Results
PDA-functionalized brackets, both freshly coated (1.08 OD) and aged for two months (1.6 OD), showed significantly reduced biofilm formation compared to non-functionalized control brackets (2.07 OD), with p-value < 0.05. This reduction was confirmed through optical density measurements and colony-forming unit (CFU) counts (1.63E + 06, 4.53E + 07, and 7.56E + 07 respectively, p-value < 0.05). SEM analysis revealed alterations in surface morphology and composition, suggesting enhanced biointeraction in the coated brackets. Stability assessments in artificial saliva and deionized water demonstrated the durability of the coating. pH measurements indicated minimal changes in SBF and water, with PDA-functionalized brackets showing slight alterations.

Conclusions
Our research findings suggest that PDA-functionalized brackets possess promising antimicrobial properties and stability, offering potential applications in orthodontic treatment to mitigate biofilm formation and prevent white spot lesions around orthodontic brackets. Further investigation is required to optimize the coating formulation and explore its long-term efficacy in clinical settings.
A lack of sexual autonomy is associated with increased loneliness in young mothers
Authors: Bunke Chelsea , Keck Tara 
Published: January 2025

Abstract
Background
Loneliness is a significant risk factor for both mental and physical health issues, including depression and increased mortality. Loneliness is reported at higher levels during life transitions, such as the transition to motherhood. Loneliness in mothers has far-reaching detrimental impacts on both mother and child, such as an increased risk of maternal depression and child abuse. Understanding the impact of different risk factors for loneliness, specifically in young mothers, may inform potential interventions for this at-risk group. The aim of this study was to determine whether mothers were lonelier than childfree women, and whether there are different risk factors for loneliness in mothers relative to childfree women, both for gender-associated and established risk factors for loneliness.

Methods
This cross-sectional study included partnered mothers and partnered childfree women between the ages of 20 and 29 from the 2020 Generations and Gender Survey (GGS) in the Republic of Moldova. The De Jong Gierveld Loneliness Scale was used to assess overall, emotional, and social loneliness. A total of 11 potential risk factors were considered, across gender, well-being, relationships, and household status. Depending on the nature of the variables and their distributions, Wilcoxon rank-sum tests or Spearman correlation coefficients were used to assess loneliness risk factors for partnered mothers and childfree women.

Results
Data from 396 mothers and 113 childfree women in the Republic of Moldova were analysed in this study. There was no significant difference between the mean overall, emotional, or social loneliness scores in partnered mothers and childfree women. A lack of sexual autonomy was a risk factor associated with social loneliness in young mothers, but not in childfree women. This was the only gendered risk factor that differed between populations. Other gendered risk factors were not significant for any types of loneliness in either population. There were differences between mothers and childfree women in several established risk factors for loneliness.

Conclusion
Mothers were not lonelier than childfree women in this study, but a lack of sexual autonomy was a risk factor associated with loneliness only in mothers.
Facilitators and barriers affecting the implementation of e-health for chronic respiratory diseases in remote settings: a qualitative evidence synthesis
Authors: Salmi Emil Matias , Basile Francesca Wanda , Khan Faiz Ahmad , Watt Larry , Song Rinn , Bijker Else Margreet 
Published: January 2025

Abstract
Background
Chronic respiratory diseases are important causes of disability and mortality globally. Their incidence may be higher in remote locations where healthcare is limited and risk factors, such as smoking and indoor air pollution, are more prevalent. E-health could overcome some healthcare access obstacles in remote locations, but its utilisation has been limited. An improved understanding of barriers and facilitators to the implementation of e-health in remote locations could aid enhanced application of these approaches.

Methods
We performed a qualitative evidence synthesis to explore factors affecting the successful implementation of e-health interventions in remote locations for patients with chronic respiratory diseases. We searched PubMed, CINAHL, Embase, Web of Science and PsycINFO databases for qualitative and mixed-methods studies. Studies were assessed by two researchers, and 41 studies were included in the synthesis. Quality was assessed via the CASP-tool. Findings were coded with Atlas.ti software and categorised based on an adapted Digital Health Equity Framework.

Results
Nineteen themes were identified across five levels (individual, interpersonal, community, society and technology), with associated facilitators and barriers for implementation. An important facilitator of e-health was its role as a tool to overcome obstacles of distance and to increase access to care and patients’ self-efficacy. Potential barriers included the reduction of in-person interactions and an increased burden of work for healthcare providers. Good quality, usability, adaptability and efficacy of e-health interventions were important for implementation to be successful, as were adaptation to the local setting — including culture and language —and involvement of relevant stakeholders throughout the process.

Conclusions
Several factors affecting the implementation of e-health in remote and rural locations for patients with chronic respiratory disease were identified. Intervention objectives, target population, geographical location, local culture, and available resources should be carefully considered when designing an e-health intervention. These findings can be used to inform the successful design and implementation of future e-health interventions.
Role of extracellular vesicles in the pathogenesis of mosquito-borne flaviviruses that impact public health
Authors: Martínez-Rojas Pedro Pablo , Monroy-Martínez Verónica , Ruiz-Ordaz Blanca H. 
Published: January 2025

AbstractMosquito-borne flaviviruses represent a public health challenge due to the high-rate endemic infections, severe clinical outcomes, and the potential risk of emerging global outbreaks. Flavivirus disease pathogenesis converges on cellular factors from vectors and hosts, and their interactions are still unclear. Exosomes and microparticles are extracellular vesicles released from cells that mediate the intercellular communication necessary for maintaining homeostasis; however, they have been shown to be involved in disease establishment and progression. This review focuses on the roles of extracellular vesicles in the pathogenesis of mosquito-borne flavivirus diseases: how they contribute to viral cycle completion, cell-to-cell transmission, and cellular responses such as inflammation, immune suppression, and evasion, as well as their potential use as biomarkers or therapeutics (antiviral or vaccines). We highlight the current findings concerning the functionality of extracellular vesicles in different models of dengue virus, Zika virus, yellow fever virus, Japanese encephalitis virus, and West Nile virus infections and diseases. The available evidence suggests that extracellular vesicles mediate diverse functions between hosts, constituting novel effectors for understanding the pathogenic mechanisms of flaviviral diseases.
Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity
Authors: Hassan Mervat G. , El-Sayyad Gharieb S. , Abdel-Monem Mohamed O. , Malash Mohamed N. , Kishk Mona A. , El Awady Mohamed E. , El-khonezy Mohamed I. 
Published: January 2025

Abstract
Background
Actinomycetes are a well-known example of a microbiological origin that may generate a wide variety of chemical structures. As excellent cell factories, these sources are able to manufacture medicines, agrochemicals, and enzymes that are crucial.

Results
In this study, about 34 randomly selected Streptomyces isolates were discovered in soil, sediment, sea water, and other environments. Using a qualitative fast plate assay, they were tested for L-glutaminase production, and nine of them produced a significant amount of pink L-glutamine. Streptomyces sp. strain 5 M was identified by examining the 16S rRNA gene in the promising strain G8. A pH of 7.5, an incubation temperature of 40 °C, and the use of glucose and peptone as the carbon and nitrogen sources, respectively, produced the highest quantities of L-glutaminase. The molecular weight of the isolated L-glutaminase was estimated to be 52 kDa using SDS-PAGE analysis. At pH 7.5 and Temp., 40 °C, the isolated enzyme exhibited its highest levels of stability and activity. The isolated enzyme’s Km and Vmax values were 2.62 mM and 10.20 U/ml, respectively. Strong toxicity against HepG-2, HeLa, and MCF-7 was observed due to the anticancer properties of the isolated L-glutaminase.

Conclusion
Our findings include the discovery of Streptomyces sp. strain 5 M, which yields a free L-glutaminase and maybe a possible applicant for extra pharmacological investigation as an antineoplastic drug.
Significance of siderophore-producing cyanobacteria on enhancing iron uptake potentiality of maize plants grown under iron-deficiency
Authors: Brick Mandees Bakr , Hussein Mervat H. , Mowafy Amr M. , Hamouda Ragaa A. , Ayyad Amr M. , Refaay Dina A. 
Published: January 2025

Abstract
Background
In response to iron deficiency and other environmental stressors, cyanobacteria producing siderophores can help in ameliorating plant stress and enhancing growth physiological and biochemical processes. The objective of this work was to screen the potential of Arthrospira platensis, Pseudanabaena limnetica, Nostoc carneum, and Synechococcus mundulus for siderophore production to select the most promising isolate, then to examine the potentiality of the isolated siderophore in promoting Zea mays seedling growth in an iron-limited environment.

Results
Data of the screening experiment illustrated that Synechococcus mundulus significantly recorded the maximum highest siderophore production (78 ± 2%) while the minimum production was recorded by Nostoc carneum (24.67 ± 0.58%). Therefore, Synechococcus mundulus was chosen for the beneficiary study and the intended agricultural application. Siderophore-type identification tests proved that Synechococcus mundulus produced hydroxamate-type. The response surface approach was successful in optimizing the conditions of siderophore production in Synechococcus mundulus with actual values for maximum biomass (387.11 mg L− 1) and siderophore production (91.84%) higher than the predicted values. The proton nuclear magnetic resonance (1H NMR) analysis data and the Fourier transformer-infrared spectrum analysis (FT-IR) signify the hydroxamate nature of Synechococcus mundulus isolated siderophore. Zea mays seedlings’ growth response in the hydroponic system was significantly stimulated in response to supplementation with Synechococcus mundulus siderophore in the absence of iron compared to plants grown without iron and the positive controls. Additionally, the contents of chlorophyll a, chlorophyll b, carotenoids, total carbohydrates, and total protein were all surpassed in siderophore-treated plants, which is expected due to the increased iron content.

Conclusions
The results introduced in this study highlighted the significant potential of Synechococcus mundulus-derived siderophore in stimulating Zea mays physicochemical growth parameters and iron uptake. Findings of this study present novel visions of cyanobacteria producing siderophores as an ecofriendly alternative candidate to synthetic iron chelators and their role in plant stress management.
The effect of an app-based dietary education on dietary intake and cardiometabolic risk markers in people with type 2 diabetes: results from a randomized controlled trial
Authors: Sjöblom Linnea , Hantikainen Essi , Dahlgren Anna , Trolle Lagerros Ylva , Bonn Stephanie E. 
Published: January 2025

Abstract
Background
mHealth, i.e. mobile-health, strategies may be used as a complement to regular care to support healthy dietary habits in primary care patients. We evaluated the effect of a 12-week smartphone-based dietary education on overall diet quality (primary outcome), and dietary intake and cardiometabolic risk markers (secondary outcomes) in people with type 2 diabetes.

Methods
In this two-armed randomized clinical trial, people with type 2 diabetes were recruited within a primary care setting and randomized 1:1 to a smartphone-delivered dietary education for 12 weeks or a control group receiving regular care only. Dietary intake and cardiometabolic risk markers were measured at baseline and after 3 months. Diet was assessed using a 4-day dietary record and a food frequency questionnaire (FFQ). Overall diet quality was estimated with a Nordic Nutrition Recommendation (NNR) score and specific dietary intake was estimated for 13 food groups/nutrients. We used linear regression models to examine differences in change from baseline to the 3-month follow-up between the intervention and control group, adjusted for baseline values of each outcome variable.

Results
The study included 129 participants (67 in the intervention group and 62 controls), of whom 61% were men. At baseline, mean age was 63.0 years and mean body mass index was 29.8 kg/m2. When analyzing dietary record data, we found no effect of the intervention on diet quality or intake, however, the control group had increased their score by 1.6 points (95%CI: -2.9, -0.26) compared to the intervention group. In the analyses of FFQ data, the intervention group had lowered their daily intake in grams of saturated (β = -4.1, 95%CI: -7.9, -0.2) and unsaturated (mono- and polyunsaturated) (β = -6.9, 95%CI: -13.5, -0.4) fat more than the control group. The intervention group also presented lower serum triglycerides levels than the controls (β = -0.33, 95%CI: -0.60, -0.05). No statistical differences were found in any other dietary variables or cardiometabolic risk markers.

Conclusion
While we found no effect on overall diet quality, our findings suggest that a smartphone-based dietary education might impact dietary fat intake and corresponding cardiometabolic risk markers in people with type 2 diabetes. Our results should be considered hypothesis-generating and need to be confirmed in future studies.

Trial registration
Registered at ClinicalTrials.gov (NCT03784612). Registered 24 December 2018.
Managing xylazine-involved overdoses in a community harm reduction setting: lessons from Tijuana, Mexico
Authors: Bufanda Lilia Pacheco , Montoya Alejando González , Carrillo Brenda Torres , Tejeda Mariana Alejandra Gonzalez , Segovia Luis A. , Calderón-Villarreal Alhelí , Friedman Joseph R. 
Published: January 2025

Abstract
Background
Xylazine is a α2-adrenergic receptor agonist, used for sedation in veterinary contexts. Although it is increasingly found in overdose deaths across North America, the clinical management of xylazine-involved overdoses has not been extensively studied, especially in community-based harm reduction settings. Here we present a clinical series of xylazine-involved overdose and share the clinical approach and lessons learned by a community overdose response team in Tijuana, Mexico amidst the arrival of xylazine.

Case Presentation
We present three cases describing the clinical management of patients with xylazine-involved overdoses that occurred in close proximity to the Prevencasa community harm reduction clinic. The long period of post-naloxone sedation in xylazine overdoses is a unique clinical feature. The first case is a 61-year-old man with longstanding opioid and methamphetamine use disorder found hypoxic, who received 4.0 mg of intranasal naloxone, and quickly began respirating well. He remained unconscious for 20 min, and upon awakening, experienced withdrawal symptoms, agitation and confusion, and exposed himself to considerable physical danger by entering a local roadway. The second is a 28-year-old man who primarily uses stimulants, who overdosed while trying “China White”. His oxygen saturation improved from 81 to 100% with supplemental oxygen and field management, and he did not require naloxone administration. He recovered consciousness after 40 min. The third patient is a 36-year-old male who was found down, saturating at 20%, who received 0.4 mg intramuscular naloxone, was placed in recovery position, and remained unconscious for 12 min before making a complete recovery. The first and third patients provided urine and drug samples that tested positive for xylazine and fentanyl.

Conclusions
We describe insights about the clinical management of combined xylazine-fentanyl involved overdoses in the field, from a community harm reduction context where xylazine arrived suddenly spurring a large number of overdoses. In response to the long period of post-naloxone sedation inherent to xylazine overdoses, the clinical team learned to center oxygenation—not consciousness—as the key parameter of interest for the titration of naloxone, increase emphasis on field airway management, portable oxygen administration, and scene safety, and employ xylazine testing strips for urine and direct substance analysis to educate the patient population about health risks.
Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavities
Authors: Sola-Leyva Alberto , Romero Bárbara , Canha-Gouveia Analuce , Pérez-Prieto Inmaculada , Molina Nerea M. , Vargas Eva , Mozas-Moreno Juan , Chamorro Clara , Saare Merli , Salumets Andres , Altmäe Signe 
Published: January 2025

Abstract
Background
A didelphic uterus represents a unique and infrequent congenital condition in which a woman possesses two distinct uteri, each with its own cervix. This anomaly arises due to partial or incomplete merging of the Müllerian ducts during the developmental stages in the womb. Accounting for uterine malformations, a didelphic uterus is a relatively rare condition, affecting approximately 0.5–2% of the population and is considered one of the more uncommon types of uterine abnormalities.

Methods
This case report aims to study the physical separation in uterine didelphys and its impact on endometrial microbiome and inflammation, and the patterns of endometrial receptivity observed.

Results
Endometrial receptivity analyses revealed a similar receptive state in both uteri, both in the early receptive phase. Differential markers of chronic endometritis, including CD138, and MUM1-positive cells, were observed when comparing endometrial biopsies from both uteri. The right uterus exhibited a higher prevalence of these positive cells. Regarding the microbiome, significant differences were found between the uteri, notably in the right uterus, a clear non-dominance of lactobacilli and the presence of genera such as Staphylococcus, Streptococcus, and Acinetobacter. Additionally, the right uterus presented a less ‘favourable’ microenvironment, a characteristic that was also reflected in the right cervix; both sites presenting less lactobacilli than the left side samples. A distinct metabolomic signature associated with the physical separation of the uteri contributed to the differences in endometrial milieu.

Conclusions
Our study revealed that physical separation, among other factors in uterus didelphys, affects the endometrial microbiome, metabolome, and inflammatory state, with significant microbiome variation observed between the uteri, although similar endometrial receptivity patterns were noted.
Weight cycling exacerbates glucose intolerance and hepatic triglyceride storage in mice with a history of chronic high fat diet exposure
Authors: Bernecker Miriam , Lin Anna , Feuchtinger Annette , Molenaar Anna , Schriever Sonja C. , Pfluger Paul T. 
Published: January 2025

Abstract
Background
Obese subjects undergoing weight loss often fear the Yoyo dieting effect, which involves regaining or even surpassing their initial weight. To date, our understanding of such long-term obesity and weight cycling effects is still limited and often based on only short-term murine weight gain and loss studies. This study aimed to investigate the long-term impacts of weight cycling on glycemic control and metabolic health, focusing on adipose tissue, liver, and hypothalamus.

Methods
Chow-fed mice and mice subjected to prolonged high-fat diet (HFD) consumption for 20 weeks, followed by 24 weeks of dietary interventions to either induce weight gain, weight loss, or weight cycling were monitored for perturbations in feeding efficiency and glucose homeostasis. Post-mortem analyses included qPCR, Western Blotting, biochemical and microscopical assessments for hepatic steatosis and insulin resistance, hypothalamic and adipose tissue inflammation, and circulating lipid, leptin and IL-6 levels.

Results
Weight cycling led to hyperphagia and rapid weight regain, matching the weights of mice continuously on HFD. Despite weight loss, adipose tissue inflammation persisted with elevated pro-inflammatory markers, macrophage infiltration, and impaired Glut4 expression. HFD-induced dysregulation in hypothalamic expression of orexigenic peptides and synaptic plasticity markers persisted also after weight normalization suggesting long-lasting neural alterations. Weight-cycled mice exhibited higher circulating IL-6 and leptin levels, increased hepatic lipid storage, and dysregulated glucose metabolism compared to those with consistent diets, indicating worsened metabolic effects by Yoyo dieting.

Conclusion
In sum, our study highlights significant metabolic risks associated with weight cycling, particularly following prolonged obesity. Persistent adipose tissue inflammation, perturbed neural peptide and plasticity markers and impaired glucose tolerance emphasize the need for effective and sustainable weight loss strategies to mitigate the adverse outcomes of weight regain and improve long-term metabolic health.