bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sow Reproductive Analysis for Local Areas and Zones via Automated Recording Information System (SALAZAR IS)
Authors: SALAZAR JOEL C. , MINGALA CLARO N. , TORRES PAUL MICHAEL S. 
Published: February 2022

Abstract
A user-friendly, prototype web-based information system (WBIS) was developed using propriety software (Windows Operating System, Internet Information Services (IIS) for the webserver, Microsoft Visual Studio C#, ASP.net framework and Microsoft SQL for Database Server) to assist the backyard hog raisers in evaluating and analyzing the reproductive performance and disorders in the farm. A farm survey was conducted to acquire basic information together with the problems and farmers’ needs. Programs for data encoding, user interface, and database backups were developed. The developed WBIS was piloted on some farms in different municipalities of the 3rd district of Pampanga, the Philippines. The result of the pilot application shown that WBIS could run on the internet on different platforms (PC/laptop, android, and IOS smartphones) and can serve as a cheaper alternative of the costly software and operating system for existing breeding monitoring system for the sow.Therefore, the WBIS, also known as Sow Reproductive Analysis for Local Areas and Zones via Automated Recording Information System, (SALAZARIS), with further improvement and updating can deliver an efficient tool and guide for backyard farmer to improve their sow’s productivity in the future.
Tumor-infiltrating NK cell subsets associated with the magnitude of T cell response in human lung cancer
Authors: awad Amiera , Ramírez-Suástegui Ciro , Clarke James , Panwar Bharat , Wood Oliver , Chee Serena , Thomas Gareth , Sanchez-Elsner Tilman , Ottensmeier Christian , Friedmann Peter , Vijayanand Pandurangan , Ganesan Anusha-Preethi 
Published: February 2022

Abstract
NK cells are the predominant innate lymphocyte subset that contribute to anti-tumor immunity. The molecular profile of human tumor-infiltrating NK cells, and particularly, their characteristics in relation to CD8+ cytotoxic T lymphocyte (CTL) density within tumors is poorly understood. Here, we performed single-cell and bulk transcriptomic analysis of NK cells within tumor and adjacent normal lung tissue from patients with treatment-naïve lung cancer. We found divergent NK cell subsets with distinct functional features that were associated with the magnitude of CD8+ CTL response in tumors. CD16pos NK cells present in tumors with high density of CD8+ CTLs were enriched for transcripts linked to cytotoxicity, pro-inflammatory chemokines and effector function. CD16neg NK cells predominantly infiltrated tumors relative to lung tissue and harbored sub-populations with unique gene expression programs. Notably, tumors with high density of CD8+ CTLs were enriched for a CD16neg NK cell subset with features of tissue residency and NK cell memory as well as a CD16neg ILC3-like subset that displayed transcriptional features linked to activation, NFκB signaling, dendritic cell recruitment and lymphoid-tissue inducing properties. These data reveal that a potentially synergistic NK cell innate immune program may co-operate with CD8+ CTLs to mediate tumor clearance in tumors with a high tumor-infiltrating lymphocyte (TIL) density.
Metabolic profile analysis based on GC-TOF/MS reveals the negative correlation between catechins and fatty acids in the cottonseed of Gossypium hirsutum
Authors: Ma Lei , Yabing Chen , Shuangjiao Xu , Ruidan Dong , Yiwen Wang , Dan Fang , Jun Peng , Tian XinQuan 
Published: February 2022

Abstract
Background: The diversified and high value-added utilization of cotton by-products can promote the sustainable development of modern agriculture. Differences in potential nutrient among varieties can be explained by variations in the composition and abundance of fatty acids, polyphenols, carbohydrates, amino acids and organic acids, etc. Therefore, the analysis of metabolite species and relationships in cottonseed is meaningful for the development of cotton by-products.Results: In this study, the metabolomes of the three cotton cultivars of different species were compared using an untargeted GC-TOF/MS analysis. A total of 263 metabolites were identified from 705 peaks and their levels were compared across the cultivars. PCA and OPLS-DA of these metabolites could clearly distinguish. There were significant differences in the content of amino acids, carbohydrates, organic acids flavonoids, and lipids in G. hirsutum TM-1 compared with G. arboretum Shixiya1 and G. barbadense Hai7124. It is noteworthy that the bioactive nutrient compound catechin obtained from the differential metabolites is significantly accumulated in TM-1. Furthermore, a comprehensive analysis using catechin and oil-related traits was conducted in core collections of Gossypium. And the results revealed the feasibility of the GC-TOF/MS analysis, as well as that catechin content has a negative association with myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid and total fatty acids.Conclusion: These fndings suggest that catechin content has a negative association with most fatty acids in cottonseed, which may provides a new solution into the underlying plant biochemistry of nutrient variation in cottonseed and pave the way to exploit the value of cotton by-products.
Artificial potential field based anti-saturation positioning obstacle avoidance control for wheeled robots
Authors: zhang pengfei , Yang Tingting , Ma Yichao 
Published: February 2022

Abstract
This paper studies the anti-saturation positioning and obstacle avoidance control of wheeled robots. First, by modifying the existing control law based on the kinematics model, a continuous function is proposed to overcome the velocity saturation problem, then the positioning control law ensuring the continuity and avoiding chattering phenomenon caused by switching control is proposed. Afterwards, a Gaussian function and a switching function are introduced to guarantee the smoothness of the artificial potential field function, and the positioning obstacle avoidance control law based on Barrier Lyapunov Function is constructed to reach the target point more stably. Finally, the effectiveness and practicability of the control algorithm are verified by simulations.
Genome-wide characterization of the Rho family in cotton provides insights into fiber development
Authors: He Man , Shang LIU , Hailiang CHENG , Dongyun ZUO , Qiaolian WANG , Limin LV , Youping ZHANG , Xingfen WANG , Guoli SONG 
Published: February 2022

Abstract
Background: Cotton provides the most natural fibers globally, and fiber development is one of the most important biological processes. Fiber develops from a single cell, and polar growth plays a vital role in fiber development. Therefore, research about cell polarity is essential to reveal fiber development mechanisms. We got inspiration from research on eukaryotes, and a key factor controlling cell polarity, Cdc-42, was focused on because of the essential role in cell polarity. Result: 2066 Rho members across eight cotton species were identified and grouped into 5 and 8 within A and D sub-genomes, respectively. Asymmetric selection of Rho members was observed among 5 tetraploids. Association analysis of previous resequenced data implied 34 Rho members in G. hirustum were related to fiber development. To verify the roles of these Rho members at the transcriptional level, previously published transcriptome data was utilized, and a weighted gene co-expression network was constructed to characterize genome-wide expression patterns. Twenty-two expressed Rho members from 5 modules were identified as functional Rho genes involved in fiber development. Interaction networks of 5 Rho members were constructed based on transcription abundance, and gene ontology enrichment showed these interacted genes participated in cell wall biosynthesis, fatty acid elongation, and other biological processes. Conclusion: Our study characterized the Rho family in cotton based on previously resequenced data and transcriptome data, providing insights on the polar growth of cotton fiber and potential application in cotton fiber improvement.
Parenchyma sparing strategy and oncological prognosis in patients with colorectal cancer liver metastases
Authors: Burlaka Anton , Makhmudov Dmytro , Lisnyi Ivan , Paliichuk Ariadna , Zvirych Vitalii , Lukashenko Andrii 
Published: February 2022

Abstract
Background: preliminary study results demonstrated parenchyma sparing surgery (PSS) as an effective approach which allowed to remove colorectal cancer (CRC) metastatic lesions within the central liver cites and increased the probability of the liver re-resections. Methods: the prospective analysis re-evaluation of the 185 CRC patients surgical treatment has been performed. Results: an overall 5-year survival (OS) of the 185 enrolled patients was 43 ± 7%, the mean and median value for OS was 48,7 ± 1.9% and 55,2 ± 5 (95%CI: 44,4 – 66,1) months. While CRC patients OS whose metastatic lesions were predominantly located within peripheral and central liver segments was 56 ± 8% and 27 ± 9% respectively (p = 0,08). A 5-year disease-free survival rates of patients with peripheral and central liver cites metastatic lesions was 31 ± 7 % and 15 ± 7%, p = 0,12. Conclusions: parenchyma sparing surgery should be a priority pathway for complex treatment of patients with deeply located lesions of the right liver lobe. Trial registration: study registered in https://www.researchregistry.com/browse-the-registry, № 5679.
The quality of working life interferes in the bioethics in physicians-patient relationship
Authors: Maluf Mario Afonso , Nunes Rui 
Published: February 2022

Abstract
Background Bioethics and Quality of Working Life for physicians constitute direct relationship. This quantitative and qualitative research has shown a close relationship between the two. Through it we seek to demonstrate that aspects that involve their work can interfere in the person of the medical professional, also directly affecting their quality in the field to the relationship with the patient, essential basis for good medicine.MethodsA structured questionnaire with topics pertinent to the context of the Quality of Working Life was sent from the Information Technology Department of the Federal Council of Medicine to the valid email addresses of 95,022 registered physicians (of the 451,777 who are active in Brazil) with a return of 426 units, 11% higher than the 383 needed to technically validate this research. The data presented were subsequently tabulated on the Excel spreadsheet, analyzed and concluded.ResultsPositive results were observed on average of the questions formulated where approximately 55% of the questions in which bioethics can be deconstructed from the context related to Quality of Working Life. ConclusionsThis study demonstrated with its positive results that, depending on the quality of work life, it also compromises more 50%, the quality of bioethics in the physicians-patient relationship, a fundamental part of the foundation of medicine. Having a new look at the medical profession is fundamental for the patients as well. This case constitutes a public health problem, since almost 90% (89.3%) of the Brazilian physicians are connected to this sector. These alarming results must be further explored and contextualized in studies carried out in other countries as well, focusing on health management, encompassing the vision in public health.
Can Fluoxetine Mitigate Mental Health Decline in Musculoskeletal Trauma Patients: A Pilot Single-Center Randomized Clinical Trial
Authors: Lossada-Soto Elizabeth , Pazik Marissa , Horodyski MaryBeth , Vasilopoulos Terrie , de Faria Ludmila Barbosa , Mathews Carol , Hagen Jennifer 
Published: February 2022

Abstract
Background

​​Musculoskeletal trauma is one of the leading causes of disability in the United States and its negative quality of life impact extends beyond that of physical recovery. More than 50% of victims of musculoskeletal trauma suffer lasting mental health issues and Post Traumatic Stress Disorder (PTSD) symptomology following their injury. These symptoms can develop across all spectrums of patients and are independent predictors of poor outcome. Access to mental health care is limited, expensive and time intensive, and a large majority of the trauma population do not get to utilize this valuable resource. This leaves the burden of management on the orthopedic team, as they are often the only point of contact for the patient within the medical system.
Methods

This is a single-center, repeated measures, randomized controlled pilot study including up to 100 orthopaedic trauma patients aged between 18-85 years of age. Subjects are approached during their index hospitalization and are randomized to one of two pharmaceutical interventions, fluoxetine (also known as Prozac) or calcium, for 9 months. Fluoxetine is a Selective Serotonin Reuptake Inhibitor (SSRI) that is supported for the treatment of PTSD by the American Psychiatric Association. It is low-cost and has minimal side effects and withdrawal symptoms if stopped suddenly. Calcium is a supplement with minimal side effects that is used in our study for its bone-healing potential. Subjects will complete physical and mental health surveys at baseline, 2 weeks, 6 weeks, 3 months, 6 months, and 1 year.
Discussion

The goals of this exploratory clinical trial are to test the effect of immediate (post-injury) treatment with fluoxetine on the development of PTSD symptomology and physical recovery for victims of musculoskeletal trauma (Aim 1). The secondary aim (Aim 2) is to develop a safe, effective and time limited protocol for use by orthopaedic providers and other non-mental health care providers treating these patients. This study is novel in that it strives to prevent the development of symptomology from the time of injury and empowers surgeons to manage their patients in a more holistic manner.
Trial registration:

ClinicalTrials.gov, NCT04850222. Registered on April 20, 2021.
Role of Soil Minerals on Organic Phosphorus Availability and Phosphorus Uptake by Plants
Authors: Amadou issifou , Faucon Michel-Pierre , Houben David 
Published: February 2022

Abstract
AimsOrganic P (OP) accounts for significant fractions of the total phosphorus (P) pool in soils, especially in soils treated with organic inputs. In soils, OP compounds can be sorbed onto soil minerals but the consequences of these interactions on P availability and plant uptake are poorly known. This research aims at elucidating the extent to which adsorbed OP compounds to major soil minerals may be available to plants.MethodsRyegrass (Lolium multiflorum) plants were grown in RHIZOtest devices in the presence of OP (myo-inositol hexakisphosphate (IHP), glycerophosphate (GLY) and glucose-6-phosphate(G6P)) and inorganic P (IP) compounds that were previously adsorbed onto Fe and Al oxides (goethite and gibbsite, respectively) and clay minerals (montmorillonite and kaolinite). Phosphorus availability and P uptake were then determined through rhizosphere and plant characterization. ResultsIrrespective of the type of mineral, ryegrass was able to take up about 3-18% of adsorbed OP compounds. The magnitude of availability and uptake depended on the OP compounds and the type of soil minerals. The potential availability of OP adsorbed by different minerals was strongly mediated by mineral-OP interaction types and properties. The P uptake increased in the following order: kaolinite-OP << gibbsite-OP ≤ goethite OP << montmorillonite-OP. Phosphorus uptake from adsorbed OP compounds showed contrasting pattern compared to adsorbed IP and depended on available P in the rhizosphere and not necessarily on the binding strength of OPs to the mineral surface.ConclusionsUnravelling the role of both OP and soil minerals properties on P availability constitutes an important knowledge to understand P dynamic in soil-plant system. Our results evidenced that the forms of OP adsorbed on Fe and Al oxides or clay minerals are available for plants to an extent which is strongly driven by OP forms and mineral types.
Iris lactea var. chinensis Plant Drought Tolerance Depends on Response of Proline Metabolism, Transcription Factors, Transporters and the ROS Scavenging System
Authors: Zhang Yue , Zhang Ruihai , Song Zhen , Fu Weidong , Yun Lingling , Gao Jinhui , Hu Guang , Wang Zhonghui , Wu Hanwen , Zhang Guoliang , Wu Jiahe 
Published: February 2022

Abstract
BackgroundIris lactea var. chinensis, a perennial herb, is widely distributed and possesses good drought tolerance traits. However, there is not much information concerning this herb in public databases, so it is difficult to understand its underlying mechanism of drought tolerance. ResultsIn this study, we conducted RNA-seq analysis of I. lactea var. chinensis plants treated under water deficiency condition and rehydration by Illumina sequencing technology to explore potential mechanisms involved in plant drought tolerance. The results showed that de novo assembly of the transcriptome generated 126,979 unigenes, of which 44,247 unigenes were successfully annotated. Among these, 1,187 differentially expressed genes (DEGs) were identified from the comparison of water deficiency treatment with the control (T/CK), including 481 up-regulated and 706 down-regulated genes. Additionally, 275 DEGs were identified in the comparison of rehydration plants and water deficiency treatment (R/T). Based on real-time quantitative RT-PCR analysis, the expression levels of eight unigenes arbitrarily selected were consistent with transcriptome data under water deficiency stress and rehydration treatment, as well as the control. According to Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway analysis, the results showed that Proline metabolism-related DEGs, including the ‘Proline catabolic process’, ‘Proline metabolic process’, and ‘arginine and Proline metabolism’, possibly play important roles in plant drought tolerance. Additionally, these DEGs were involved in 43 transcription factors, 46 transporters, and 22 reactive oxygen species (ROS) scavenging system-related proteins. Biochemical analysis and histochemical detection showed that Proline and ROS were accumulated under water deficiency treatment, consistent with transcriptomic analysis. ConclusionsIn sum, our transcriptome data revealed that the plant drought tolerance of I. lactea var. chinensis depends on Proline metabolism, the action of transcription factors and transporters, and a strong ROS scavenging system, which related genes found in this study could be a useful molecular breeding resources to enhance I. lactea var. chinensis drought tolerance.
Holocene paleolimnological changes in Rundvågshetta lakes of the Soya Coast region and their paleoenvironmental significance with glacio-isostatic uplift in East Antarctica
Authors: INOUE Genki , Honda Eisuke , Ito Keisuke , Kang Ijin , Seto Koji , Tani Yukinori , Watanaabe Takahiro , Kashima Kaoru , Ohtani Shuji , Yamanaka Toshiro , Nakamura Toshio , Takano Yoshinori , Imura Satoshi 
Published: February 2022

Abstract
We studied Holocene paleolimnological changes inferred from multi-proxy data set of CNS elements, biomarkers and microscopic observation of microalgae and cyanobacteria in sediment cores from Rundvågshetta lakes (Maruwanminami-ike and Maruwan o-ike) in the Soya Coast region of East Antarctica, along with sedimentary facies and AMS 14C dating. They are discussed with paleoenvironmental changes, transition ages and glacio-isostatic uplift rates in the Soya Coast region and East Antarctica. Ages of the Maruwanminami-ike core (MwS4C-01, length 147 cm) and Maruwan-oike (Mw4C-01, length 226 cm) ranged from 1,230-5,010 cal BP) and 2,240-5,700 cal BP, respectively. Reservoir effects of the Rundvågshetta lakes were very high which may be due to the influence of dead carbon in glacially eroded marine sediments and base rocks. Average sedimentation rates of Lakes Maruwanminami-ike and Maruwan-oike were 0.389 and 0.649 mm/yr, respectively. Crustal uplift rates of Lakes Maruwanminami-ike and Maruwan-oike determined by sedimentary facies, green sulfur bacterial biomarkers and diatoms were estimated to be 8.24 and 8.25 mm/yr, respectively. Coastal marine environment: Maruwanminami-ike (147-72.5 cm, 5,010-2,590 cal BP) and Maruwan-oike (226-47.2 cm, 5,700-3,190 cal BP) were characterized by low biological production with the predominance of diatoms. Transition period of stratified brackish lake environment: Maruwanminami-ike (72.5-65.6 cm, 2,590-2,500 cal BP) and Maruwan-oike (47.2-28.8 cm, 3,190-2,890 cal BP) were characterized by stratified conditions with marine water overlain by freshwater, and a chemocline developed together with an anoxic layer in the bottom of photic zone. Freshwater lake environment of Maruwanminami-ike (65.6-0 cm, 2,500-1,230 cal BP) was characterized by high biological production by cyanobacteria (e.g. Leptolyngbya spp.) and green algae (e.g. Comarium clepsydra) with some contribution of diatoms, while that of Maruwan-oike (28.8-0 cm, 2,890-2,240 cal BP) was very low biological production. The marine to terrestrial transition ages of raised beaches and isolated basins in the Saya Coast region ranged from 970 to 9,290 yr BP with an average of 3,660±1,520 yr BP (standard deviation) suggesting that major warm periods in the region are the middle Holocene. Glacio-isostatic uplift rates of raised basins and isolated basins in the Soya Coast region ranged from 0.19 to 4.40 mm/yr with an average of 2.0 ± 0.92 mm/yr which is similar to East Antarctica. Glacio-isostatic uplift rates of raised beaches are correlated with altitudes with a correlation coefficient of r2 = 0.724. Increasing crustal uplift rates with altitudes reflect continuous crustal uplifts in the Soya Coast region.
Hazard and risk assessment for early phase road planning in Norway.
Authors: Solheim Anders , Sverdrup-Thygeson Kjetil , Kalsnes Bjørn 
Published: February 2022

Abstract
Road construction in rugged terrain and variable, partly harsh climate is challenging. Proper assessment of natural hazards at an early planning stage can give large cost savings and safer roads. In assessing natural hazards along 720 km of planned roads in Norway, a GIS based tool was developed to utilize publicly available data and dynamic runout models. The output is a definition of the most critical locations and serves to limit the extent of necessary field work. The Norwegian national susceptibility maps are generally conservative and using only these in the planning would give unrealistically high hazard levels. Various optimizing techniques were therefore implemented in the GIS tool and the outputs further calibrated against existing detailed hazard maps in selected locations and further validated during field work at the defined 'hotspots'. The field work comprised assessing return periods of unwanted events, probable closure time, and relevant mitigation measures, all within sets of pre-defined ranges of values. The following consequence evaluation quantified the indirect economic consequences of closed road and assessed the consequence for emergency preparedness qualitatively. Other consequences were not considered in the study. Climate change was considered and evaluated to increase the probability for flooding and debris flows, whereas the link between climate change and the other assessed hazards were considered too uncertain to impact on the risk estimates. Results of the study were communicated through an interactive map solution, with key results presented as fact sheets activated in the map for each risk section of the roads.