bluuebunny/crossref_metadata_2025_split huggingface dataset:
circCUL1 promotes autophagy in trophoblast cells via the miR-30e-ATG7/14 and ANXA1/PI3K/AKT pathways
Authors: Yang Tong , Hu Jianguo , Zhang Lei , Liu Li , Pan Xin , Zhou Yanqiu , WU Yi , Shi Xian , Dong Xiaojing 
Published: May 2022

Abstract
Background
Fetal growth restriction seriously affects the health of newborns. However, the cause of FGR is unclear. Circular RNAs (CircRNAs) are a novel type of endogenous noncoding RNAs that regulate target gene expression by interacting with microRNA (miRNA) .The present research was major in investigating the regulation association among circCUL1, miR-30e, ATG7/14 and MAPK1 in FGR, and we explored the mechanism underlying pathogenesis of FGR
Results
circCUL1 promotes autophagy in placental trophoblast cells. Mechanism studies have found that circCUL1 regulates trophoblast autophagy through the miR-30e-ATG7/14 and ANXA1/PI3K/AKT pathways. We also observed that knocking out circCUL1 promoted mouse development.
Conclusions
our research suggested that circCUL1 may be involved in the pathogenesis of FGR. circCUL1 may be a potential diagnostic marker and therapeutic target of FGR.
Clinical characteristics and long-term outcomes of individualized re-treatment in locoregionally recurrent nasopharyngeal carcinoma
Authors: Niu Xiaoshuang , Liu Peiyao , Xue Fen , Hu Chaosu , He Xiayun 
Published: May 2022

Abstract
OBJECTIVES
To evaluate clinical characteristics and long-term outcomes of individualized re-treatment in patients with different locoregionally recurrent patterns of nasopharyngeal carcinoma (NPC).
MATERIALS AND METHODS
From January 2005 to December 2018, sixty-five locoregionally recurrent NPC patients were included in the study. The location of recurrence determined different treatments with radiotherapy (RT) and/or surgery.
RESULTS
The median age at the time of re-treatment was 51 years old. Twenty-nine (44.6%) patients had tumors classified as T3-4 and forty-four (67.7%) patients had tumors classified as N2-3 in the initial treatment. All patients were previously treated with intensity-modulated radiotherapy (IMRT) to a median dose of 66Gy (range, 66–70.4Gy). The median time between initial treatment and recurrence was 25 months (range: 10–105 months). Tumor relapse was classified as rT3-4 in 19 patients and rN2-3 in 8 patients. The median interval between the date of locoregional relapse to the date of death or last follow-up was 28 months (range: 0 to 112 months). The overall survival (OS) at 1-year, 3-years, and 5-years was 94.4%, 67.3%, and 47.2%, respectively. Age at the time of recurrence (> 45 years) altered the OS significantly (P = 0.007).
CONCLUSIONS
Locoregionally recurrent NPC should be individualized re-treatment. Therapy modality according to the recurrent site achieved relatively satisfactory long-term survival outcomes. Age at the time of recurrence (> 45 years) served as negatively prognostic factor of OS.
Marine biomolecule mediated synthesis of selenium nanoparticles and their antimicrobial efficiency against fish and crustacean pathogens
Authors: Rajashree Radhika , S Gayathri , M Gobalakrishnan 
Published: May 2022

Abstract
Recent techniques of intensive and super intensive aquaculture which involves high rates of stocking and supplementary feedings has substantially enhanced the fish and prawns diseases. Metallic nanoparticles could serve as future substitutes for some conventional antibiotics due to their increase antimicrobial efficiency. The present study reports a simple phytochemical mediated green synthesis of selenium nanoparticles (SeNPs) and evaluates its potential biological application as antimicrobial agent against important fish and crustacean pathogens Vibrio harveyi and Vibrio parahaemolyticus. The work focused on synthesis and characterization of SeNPs by marine macro algae Kappaphycus alvarezii(K.alvarezii) and Halimeda opuntia (H.opuntia) .These nanoparticles were characterized by Scanning Electron Microscopy (SEM) ,UV-Visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR). FTIR spectrum confirms the presence of various functional groups in the plant extract, which may possibly influence the reduction process and stabilization of nanoparticles. Crystal phase composition and average crystalline size of synthesized SeNPs were confirmed with XRD measurements. Characterization of extracted nanoparticles for shape, size, and purity indicated that the particles were spherical with diameters ranging between 30 and 80 nm. Antimicrobial study was done and the results was shown to confirm the activity against marine pathogenic microbes V. harveyi and V. parahaemolyticus. It was found that SeNPs are able to inhibit the cell growth by dose-dependent manner. The ABTS and DPPH assay results shown that the SeNPs prepared using marine macroalgal extracts possess more antioxidant potential among which K.alvarezii shown higher ant oxidative activity when compared to H. opuntia.
Distributed Processing of Ocean Spatiotemporal Data: A Survey
Authors: Li Xiaoyong , Gu Jingyun , Tan Guolong , Jiang Wenjing , Cui Ao , Shu Leiming , Ren Kaijun , Zhu Haoyang , Xu Zichen 
Published: May 2022

Abstract
Ocean data exhibits interesting yet human critical features affecting all creatures around the world. Studies on Hydrology and Oceanology become the root of many disciplines, including global resource management, macro economy, environment protection, climate predictions, etc, which motivates our further exploration on the underlying feature behind the ocean data. However, with high dimensionality, large quantities, heterogeneous sources, and especially, the spatiotemporal manner, the diversity between the specific knowledge required and massive data chunk puts forward unique challenges in data representation and knowledge mining, effectively. This paper tends to provide a summary of studies on these issues, including the data representation, data processing, knowledge discovery, and algorithms on finding unique patterns on ocean environment changes, such as temperature, tide height, waves, salinity, etc. In detail, we comprehensively discuss about ocean spatiotemporal data processing techniques. We further summarize related representation works on ocean spatiotemporal data, the construction of a ocean knowledge graph, and the management of ocean spatiotemporal data. At last, we combine and compare the collection of the evolution and multiple state-of-the-arts on ocean spatiotemporal data processing.
A Low-Profile Wideband Compressed Single-arm Spiral Antenna Array for Mid-band 5G Beam Steering Applications
Authors: Falkner Benjamin J. , Zhou Hengyi , Zhang Wei , Metaxas Georgios , Mehta Amit , Ziegler Volker , Multerer Thomas , Mirshekar-Syahkal Dariush , Nakano Hisamatsu 
Published: May 2022

Abstract
A low profile wideband spiral antenna array is presented for global mid-band 5G beam steering applications. In the global rollout of mid-band 5G, different frequencies have been licensed within each region (e.g. 3.4–3.8 GHz in the EU and 3.7–5 GHz in the USA). Therefore, antenna arrays must be able to cover a bandwidth of 3.3 GHz to 5 GHz to provide true global coverage. Initially, this work presents the design of a wideband compressed spiral antenna that provides an axial beam throughout its operational bandwidth of 3.3 GHz to 5 GHz, enabling beam steering functionality. Then, this antenna has been placed in a 4 x 4 array with a triangular lattice. The proposed spiral antenna array can provide a scanning range of -40⁰ ≤ θ ≤ +40⁰ in all azimuth directions with an average back lobe level of less than − 9.5 dB. This development will allow for low-cost integration of 5G systems for global use, such as passenger aircraft, UAVs, drones, and marine and ground vehicles.
Designing of Immunodiagnostic Assays Using Polyclonal Antibodies for Detection of Shiga Toxin Producing Pathogenic E. coli (STEC) Strains
Authors: Moges Biniam , Yinur Degisew , Hassen Aliy , Sisay Tesfaye 
Published: May 2022

Abstract
Shiga toxin-producing Escherichia coli (STEC) are the leading pathogenic strain causes of diarrhea diseases and death of humans in worldwide. Many diagnostic assays have been developed to aid for the diagnosis of STEC strain; however, they have different limitations. Thus, this study was aimed at designing rapid, effective, sensitive and specific immunodiagnostic assay for STEC strain detection. Thus, a STEC isolate available was recovered from freezer in EMB agar; then a pure typical growth was inoculated into TSB overnight at 37oC. The broth culture was processed using chloroform methanol extraction method for purification of LPS from the bacteria. Assessment on SDS-PAGE showed that the LPS preparation was contaminated by small amount and identity of cellular proteins. The produced antibody showed positive agglutination both on the purified LPS as well as the STEC isolate carrying LPS on their surface; however, agglutination of STEC was more pronounced. Mice immunized with LPS produced highest agglutination on tertiary immunization showing the progressive buildup of the antibody response against the antigen. Cultures from TSA and when they refresh showed better agglutination than cultures on EMB as well as TSB. Immunodiagnostic assay developed in this study could detect STEC strains including STEC in human feces with rapidly (1–2 minutes), sensitively (90.2%), specifically (89.5%) and accurately (90.6%). However, further studies are still required to improve it sensitivity, specificity and reproducibility. Overall this diagnostic assay provided promising results that may curb current problem with detection methods in clinical health care and research laboratories.
Resilience in older adults despite COVID-19. A multidimensional approach
Authors: Noriega C. , Velasco C. , Carretero I. , López-Frutos P. , Galarraga L. , Pérez-Rojo G. , López J. 
Published: May 2022

Abstract
Researchers have mainly focused on aging risk factors and COVID-19 consequences. However, older adults have proved their ability to overcome adversities along their life. Resilience is a protective variable that dampens the impact of stress. Based on MacLeod´s et al. (2016) approach, we aimed to analyze the relationship between older adults´ resilience and COVID-19 related-stressors as well as their physical, mental, and social characteristics. 889 people aged 60 and over participated in this study. Older participants, women, having better perceived health and not losing a loved one because of the virus were associated with more resilience. Moreover, higher levels of gratitude, personal growth, life purpose and lower levels of depression were associated with greater scores in resilience. This study offers a change of perspective in which aging is perceived from a positive viewpoint by focusing on resources that may help older adults to cope with adverse situations.
Novel treatment of small and large artery calcific disease via epigenetic activation of autophagy initiation genes
Authors: Cardenas christian Lino , Jiang Wanlin , Cherbonneau Francois , Shahrooz Zarbafian , Nicholson Christopher , Chou Elizabeth , Li Rebecca , Boerboom Sophie , Ostrom Katrina , Ichinose Fumito , Bloch Donald , Nigwekar Sagar , Ellinor Patrick , Musolino Patricia , Lindsay Mark , Miller Clint , Malhotra Rajeev 
Published: May 2022

Abstract
Vascular calcification is a hallmark of atherosclerotic disease and serves as a strong predictor and risk factor for cardiovascular events1. Growing evidence points towards macroautophagy (herein referred to simply as autophagy), a cellular self-degradation process, as being protective during early atherosclerosis2. However, autophagy may become dysregulated with advanced atherosclerosis3. Vascular smooth muscle cells (VSMC) contribute to early and advanced atherosclerosis by regulating vascular remodeling and calcification. The precise effects of autophagy on VSMC-mediated calcification are unknown. Here, we combined multi-omic profiling and high-resolution structural imaging to pinpoint an epigenetic mechanism by which impaired autophagy drives VSMC calcification. Our studies revealed dysfunctional autophagy initiation4 to be responsible for altered autophagy flux during VSMC calcification. In vivo pharmacologic and genetic activation of autophagy using chromatin modulator GSK343 and Becn1F121A knock-in mice, respectively, inhibited vascular calcification and improved survival rates in mouse models of spontaneous large (Mgp-/-) and small (Abcc6-/-) artery calcification. Taken together, these data enhance our mechanistic understanding of vascular calcification by specifically implicating impairment in the initiation of the autophagy pathway and provide important insights for a broad range of cardiovascular diseases involving VSMC phenotype switch.
Cytokine Changes in Cerebrospinal Fluid Following Vascular Surgery on the Thoracic Aorta
Authors: Pereira Christopher , Perera Anisha H. , Rudarakanchana Nung , Harris Benjamin H. L , Giovannantonio Matteo , Taylor-Robinson Simon D. , Dani Melanie , Fertleman Michael 
Published: May 2022

Abstract
Background: There is growing evidence that surgery can drive an inflammatory response in the brain. However, the mechanisms behind this response are incompletely understood. Neuroinflammation may contribute to peri-operative neurocognitive disorders (PND), including post-operative delirium (POD). Here, we investigate the hypotheses that 1. Cerebrospinal fluid (CSF) cytokines increase after vascular surgery and 2. That these changes in CSF cytokines are interrelated. Methods: Patients undergoing either open or endovascular elective surgery of the thoracic aorta were invited to participate in this study. Cerebrospinal fluid samples were taken before surgery and on the first post-operative day. These were analysed for the presence of ten cytokines by immunoassay to examine for post-operative changes in cytokine levels. Results: After surgery, there were significant increases in six out of the ten measured CSF cytokines (IL-1β, 2, 6, 8, 10 and 13). This included changes in both putative pro-inflammatory (IL-1β, 6 and 8) and putative anti-inflammatory (IL-2, 10 and 13) cytokines. The greatest increases occurred in IL-6 and IL-8, which showed a 63-fold and a 31-fold increase respectively. There was strong intercorrelation between CSF cytokines after the operation. Conclusions: Following surgery on the thoracic aorta, there was a marked increase in CSF cytokines, consistent with a potential role in neuroinflammation. The ten measured cytokines showed intercorrelation after the operation, indicating that a balance between multiple pro- and anti-inflammatory cytokines may be present. This could be detrimental, protective, or both.
Formation of Lunar Basins from Impacts of Leftover Planetesimals
Authors: Nesvorny David , Roig Fernando , Vokrouhlicky David , Bottke William (Bill) , Marchi Simone , Morbidelli Alesandro , Deienno Rogerio 
Published: May 2022

Abstract
The Moon holds important clues to the early history of the Solar System. The large scars evident on its
near side are impact basins filled with dark basalt; some 50 lunar basins (crater diameter $D>300$ km) have been
recognized overall$^1$. The question of the origin of basin-forming impactors remained unsettled. Here we show
that most impactors were rocky planetesimals left behind at $\sim 0.5$--$1.5$ au after the terrestrial planet
accretion. The number of basins expected from impacts of leftover planetesimals largely exceeds the number of
known lunar basins, suggesting that the first $\sim200$ Myr of impacts is not recorded on the lunar surface$^{2,3}$.
The Imbrium basin formation (age$^4$ $\simeq 3.92$~Gyr; impactor diameter$^{5,6}$ $d>100$ km) occurs with a
15--35\% probability in the model. Imbrium must have formed unusually late to have only two smaller basins
(Orientale and Schr\"odinger) forming afterwards. The model predicts $\simeq 20$ $d>10$-km impacts on the Earth
2.5--3.5 Gyr ago (Ga), which is comparable to the number of known spherule beds in the late Archean$^7$.
Short-Term Changes Detected by Corneal Topography and Optical Coherence Tomography After Prophylactic Laser Iridotomy in Primary Angle Closure Suspect
Authors: Şahin Ömer Faruk , Değirmenci Mehmet Fatih Kağan , Bahar Alperen , Isik Mehmed Ugur 
Published: May 2022

Abstract
Purpose: To evaluate early changes in anterior segment parameters by Scheimpflug-Placido disc topography system and changes in retinal layers by optical coherence tomography in primary angle-closure suspects after laser peripheral iridotomyMethods: One eye of 26 patients with primary angle closure suspect and of 20 healthy subjects were included in this retrospective cross-sectional study. Anterior chamber depth/volume, iridocorneal angle and central corneal thickness were obtained by Scheimpflug-Placido disc topography system. Retinal thickness, retinal nerve fiber layer thickness and ganglion cell-inner plexiform layer thickness were acquired by optical coherence tomography. All the tests were repeated 1 week and 1 month after laser peripheral iridotomy.Results: The mean ages of the patients and healthy controls were 64.8±10.7 years and 64.5 ± 3.9 years, respectively (p=0.990). Anterior chamber depth/volume and iricorneal angle value were lower in the PACS group (p<0.001, for all). Anterior chamber volume and iridocorneal angle increased significantly after laser peripheral iridotomy (p=0.004, for both). While foveal thickness decreased significantly after laser peripheral iridotomy (p=0.027), retinal nerve fiber layer thickness increased in superior and temporal quadrants (p=0.038 and p=0.016, respectively).Conclusion: Our results suggest that LPI in patients with PACS provides improved retinal thickness and RNFL thickness, as well as anterior chamber parameters.
Choice of antibody is critical for specific and sensitive detection of androgen receptor splice variant-7 in circulating tumor cells
Authors: Khan Tanzila , Lock John , Ma Yafeng , Harman David , Souza Paul , Chua Wei , Balakrishnar Bavanthi , Scott Kieran F. , Becker Therese M. 
Published: May 2022

Abstract
Background: Androgen receptor variant 7 (AR-V7) is an important biomarker to guide treatment options for castration-resistant prostate cancer (CRPC) patients. Its detectability in circulating tumour cells (CTCs) opens non-invasive diagnostic avenues. While detectable at the transcript level, AR-V7 protein detection in CTCs may add additional information. Aims: The aim of this study was to compare commercially available anti-AR-V7 antibodies and establish reliable AR-V7 immunocytostaining applicable to CTCs from prostate cancer (PCa) patients.Methods: We compared seven AR-V7 antibodies by western blotting and immmunocytostaining using a set of PCa cell lines with known AR/AR-V7 status. The emerging best antibody was validated for detection of CRPC patient CTCs enriched by negative depletion of leucocytes.Results: The anti-AR-V7 antibody, clone E308L emerged as the best antibody in regard to signal to noise ratio with a specific nuclear signal. Moreover, this antibody detects CRPC CTCs more efficiently compared to an antibody previously shown to detect AR-V7 CTCs. Conclusion: We have determined the best antibody for AR-V7 detection of CTCs, which will open future studies to correlate AR-V7 subcellular localisation and potential co-localisation with other proteins and cellular structures to patient outcomes.
Development of peptide-based biosensors for measuring cross-linking and deamidation activities of transglutaminases
Authors: Tatsukawa Hideki , Aoyama Ruriko , Hitomi Kiyotaka 
Published: May 2022

Abstract
Transglutaminases (TGs) are a protein family that catalyzes isopeptide bond formation between glutamine and lysine residues of various proteins. There are eight TG isozymes in humans, and each is involved in diverse biological phenomena due to their characteristic distribution. Abnormal activity of TG1 and TG2, which are major TG isozymes, is believed to cause various diseases, such as ichthyosis, and celiac disease. To elucidate TGs’ mechanisms of action and develop new therapeutic strategies, it is essential to develop bioprobes that can specifically examine the activity of each TG isozyme, which has not been sufficiently studied. We previously have identified several substrate peptide sequences containing Gln residues for each isozyme and developed a method to detect isozyme-specific activities by incorporating a labeled substrate peptide into lysine residues of proteins. We prepared the fluorescein isothiocyanate (FITC)-labeled Gln substrate peptide (FITC-K5 and FITC-T26) and Rhodamine B-labeled Lys substrate peptide (RhoB-Kpep). Each TG reaction specifically cross-linked these probe pairs, and the proximity of FITC and Rhodamine B significantly decreased the fluorescence intensity of FITC depending on the concentration and reaction time of each TG. In this study, we developed a peptide-based biosensor that quickly and easily measures TG isozyme-specific activity. This probe is expected to be helpful in elucidating TG’s physiological and pathological functions and in developing compounds that modulate TG activity.
Hydride-ion conductivity and enhanced atmospheric stability of hydride-fluoride SrMgH4–xFx
Authors: Ayu Nur , Matsui Naoki , Jiang Guangzhong , Hirose Takashi , Itoh Takashi , Mezaki Takeya , Toda Yoshitake , Suzuki Kota , Hirayama Masaaki , Saito Takashi , Kamiyama Takashi , Kanno Ryoji 
Published: May 2022

Abstract
Few hydride-ion conductors exhibit chemical stability and hydride-ion conductivity, which are essential properties in developing hydride-ion-driven electrochemical devices. Herein, we successfully synthesized a novel hydride-fluoride system, SrMgH4 − xFx, possessing high atmospheric stability and relatively high hydride-ion conductivity. The correlation between anion composition, structure, hydride-ion conductive properties, and atmospheric stability in the hydride-fluoride system was elucidated. The doping of fluoride ions at hydrogen sites resulted in high atmospheric stabilities while retaining hydride-ion conductivities of SrMgH2F2 and SrMgHF3 of 3.4 × 10− 4 and 1.4 × 10− 4 S cm− 1 at 400°C, respectively. For SrMgHF3, in particular, 84% ionic conductivity was retained after exposure to the atmosphere. Hydride-fluoride systems enable the balancing of the ionic conductivity and atmospheric stability by controlling the H/F ratio. Our findings provide insight for use in effective material design to expand the use of metal hydrides in practical solid electrolytes for use in hydride-ion-driven devices.
Heat transfer performance and prediction of open pulsating heat pipe for self-cooling cutting tool
Authors: Wu Ze , Bao Hang , Xing Youqiang , Liu Lei 
Published: May 2022

Abstract
The pulsating heat pipe (PHP) can be used to transfer massive heat to reduce the thermal damage to the cutting tool when machining difficult-to-cut materials. To select a better open PHP, the heat transfer performance was experimentally investigated in this study. The operating characteristics of different types of working fluids under different heat flux were analyzed compared with the closed PHP. Visual experiments were established to verify the experimental characteristics. The effects of heat flux, length, ratio of inner/outer diameter, and inclination angle on equivalent thermal resistance were analyzed. Based on the experimental data and four boosting integrating learning methods, a model for heat transfer performance prediction was proposed. The prediction model based on the CatBoost method had better goodness-of-fit and the best prediction effect. The R2, MAPE, and RMSE of the validation set were the best, which are 0.9258, 7.2564, and 0.1057 respectively. In addition, the contribution of input parameters to the output results was evaluated, while L, Di/Do, and Pr were the top three variables. Sub-tree structures used to explain the prediction model were also presented. This proposed prediction model can be used to select the most suitable open PHP before designing PHP self-cooling tools.