bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic factors of gliosarcoma in the real world: a retrospective cohort study
Authors: Yu Ziye , Zhou Zhirui , Xu Ming , Song Kun , Shen Jingjing , Zhu Wenhao , Wei Liqun , Xu Hongzhi 
Published: April 2022

Abstract
Purpose: Gliosarcoma is a histopathological variant of glioblastoma, which is characterized by a biphasic growth pattern consisting of glial and sarcoma components. Owing to its scarcity, data regarding the impact of available treatments on the clinical outcomes of gliosarcoma are inadequate. The purpose of this retrospective cohort study was to analyze the prognostic factors of gliosarcoma.Methods: By screening the clinical database of neurosurgical cases at a single center, patients with gliosarcoma diagnosed histologically from 2013 to 2021 were identified. Clinical, pathological and molecular data were gathered founded on medical records and follow-up interviews. Prognostic factors were derived using Cox proportional hazards model with backward stepwise regression analysis.Results: Forty-five GSM patients were included. Median overall survival was 25.6 months (95%CI 8.0–43.1), and median relapse free survival was 15.2 months (95%CI 9.7–20.8). In multivariable analysis, total resection (p=0.023, HR=0.192, 95%CI 0.046–0.797) indicated an improved prognosis. And low expression of Ki-67 (p=0.059, HR=2.803, 95%CI 0.963–8.162) would be likely to show statistical significance. However, there might be no statistically significant survival benefit from radiotherapy with concurrent temozolomide (n=33, 73.3%, log-rank p=0.99) or adjuvant temozolomide (n=32, 71.1%, log-rank p=0.74). Conclusion: This single centre retrospective study with a limited cohort size has demonstrated the treatment of gross total resection and low expression of Ki-67 are beneficial for patients with GSM, while radiotherapy or temozolomide is not.
Clinical trial for evaluating the effectiveness and safety of a new dental plaque removal device: Microscale mist unit
Authors: Hihara Hiroki , Izumita Kuniyuki , Iwatsu Misato , Sato Tomoya , Tagaino Ryo , Shobara Kenta , Shinohara Yuta , Hatakeyama Takanori , Kayaba Chie , Sato Mariko , Tokue Ayako , Sugawara Tomoko , Ashino Kanamai , Ikeda Koji , Aida Jun , Sasaki Keiichi 
Published: April 2022

Abstract

Objectives: The purpose of this study is to evaluate the effectiveness and safety of a microscale mist unit (MSM-UNIT), a newly developed dental plaque removal device that sprays high-speed fine water droplets, with regard to removal of dental plaque adhering to the oral mucosa (tongue, palate) and tooth surface.Materials and Methods: Fifteen patients who had difficulty self-managing sufficient oral care were included in this study. Effectiveness was evaluated in at least five patients per tongue, palate mucosa, and tooth surface, and the safety evaluation was conducted at all three sites for all patients. Effectiveness was evaluated using the rate of degree of dental plaque removal, and safety was evaluated using a numerical rating scale (NRS) for pain and symptoms of inflammation. An operator who performs treatment and evaluator who evaluates effectiveness and safety were designated. In addition, as a third party, an image judgment committee judged the effectiveness.Results: Although the evaluation for the tongue varied between the evaluator and the image judgment committee, after applying the MSM-UNIT, the rate for all degrees of plaque removal increased in all regions. In addition, low pain NRS and symptoms of inflammation were observed and within acceptable range.Conclusion: The MSM-UNIT can be used effectively and safely for removing oral plaque not only from teeth but also from the oral mucosa.Clinical Relevance: The MSM-UNIT may be used in whole oral care including for the removal of oral membranous substances in nursing and hospitalized patients.Trial registration: This study was registered on the Japan Primary Registries Network (JPRN) of University Hospital Medical Information Network Center (UMIN-CTR). The registration number was JPRN-UMIN000035950 (date of first registration: 01/03/2019).
A 2.5-cm single-port video-assisted thoracoscopic surgery for Stage III tuberculous empyema—a case report
Authors: yang zhi , chang xiujun 
Published: April 2022

Abstract
Background: Stage III tuberculous empyema is a common disease of tuberculosis, Traditionally,it has been treated by thoracotomy or video-assisted thoracoscopic surgery with two to four incisions. But conventional surgery has large trauma, large bleeding volume and long recovery time. To our knowledge it is the first report of surgery for stage III tuberculous empyema with a mini single-port approach.Case presentation: A 23-year-old woman admitted to our hospital with complaints of intermittent chest pain for half a year. We got the diagnosis of stage III tuberculous empyema after medical treatment. Considering that the patient was young and unmarried, we decided to perform minimally invasive pleural decortication through a 2.5 cm single incision. The operation time was 240 min, and blood loss was 100ml. The patient recovered well and postoperative pain was mild.Conclusion: This case demonstrates that single-port VATS with a smaller incision for the Stage III tuberculous empyema should be considered in well selected patients.
Distribution of Microplastics in Beach Sand on the Can Gio Coast, Ho Chi Minh City, Vietnam
Authors: Nhon Nguyen Thi Thanh , Nguyen Nguyen Thao , Hai Ho Truong Nam , Hien To Thi 
Published: April 2022

Abstract
Microplastics pollution in Vietnam has received significant attention in recent years because of its adverse effects on the environment. This study examined the abundance, physical characteristics, and chemical composition of microplastics in beach sand from the Can Gio Coast, Ho Chi Minh City, Vietnam for the first time. Five beaches with different features and anthropogenic activities along the Can Gio Coast were selected. Ninety sand samples were collected from the edge of the water to the upper shoreline at different depths to assess the spatial distribution of microplastics. Microplastics were extracted by density separation in a solution of sodium chloride (NaCl) and zinc chloride (ZnCl2) and examined by attenuated total reflection-Fourier transform infrared spectroscopy (FTIR-ATR). The abundance of microplastics varied from 0 to 6.58 pieces/kg d.w. Microplastics were detected mostly along the upper shoreline and in the surface sand layer. The dimension of the microplastics ranged from 2.8 to 5 mm (71.4%), granules accounted for the highest proportion of shape (42.9%), and white and blue were the two most prevalent colors (81%). Polypropylene, polystyrene, and polyethylene were the three most common polymer types. The characteristics of microplastics indicate that their origin may be from resin pellets, tourism activities, and aquacultural activities.
Identification of functional genes in liver fibrosis based on bioinformatics analysis
Authors: Zhang Feng , Pei Siya , xiao meifang 
Published: April 2022

Abstract
Liver fibrosis is a major global health problem; nevertheless, its molecular mechanisms are not completely clear. The roles of long non-coding RNAs (lncRNAs) and their related competing endogenous RNAs (ceRNA) networks in liver fibrosis are unknown. This study aimed to build a lncRNA-miRNA-mRNA network, identify potentially related lncRNAs, and explore the pathogenesis of liver fibrosis. We used the Gene Expression Omnibus databases and bioinformatics analysis to identify differentially expressed genes (DEGs) between liver fibrosis and normal tissues. The ceRNA network was constructed according to the interactions between DElncRNA, miRNA, and DEmRNA. Then, these DEGs were identified using functional enrichment analysis, and a protein-protein interaction (PPI) network was established. The critical lncRNAs were verified using the quantitative real-time polymerase chain reaction (qRT-PCR). We firstly analyzed the different expression genes (DEGs) between liver fibrosis and normal tissues based on TCGA database, and a total of 210 differentially expressed lncRNAs and 333 differentially expressed mRNAs were obtained. The ceRNA network was composed of three lncRNAs, five miRNAs, and 93 mRNAs. Gene Ontology functional enrichment analysis revealed significant enhancement in cell components, molecular function, and biological process. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed pathways associated with transcriptional misregulation in cancer, including the Rap1 signaling pathway, proteoglycans in cancer, mineral absorption, HTLV-l infection, and central carbon metabolism in cancer. According to the PPI network and the GSE84044 database, 7 hub genes associated with liver fibrosis were identified. In addition, qRT-PCR revealed that lncRNA AC100861 (lncRNA TNFRSF10A-DT) was explicitly decreased in liver fibrosis tissues and activated hepatic stellate cells. In conclusion, this study preliminarily found that lncRNA TNFRSF10A-DT may be a biomarker for the diagnosis and outcome of liver fibrosis. lncRNA TNFRSF10A -DT /has -mir- 873-5/RCC2, UBR4, and the AKR7A2 axis may be involved in liver fibrosis.
Long-Term Cardiovascular outcomes Differ Across Metabolic Dysfunction-Associated Fatty Liver Disease Subtypes
Authors: Lee Hokyou , Lim Tae Seop , Kim Seung Up , Kim Hyeon Chang 
Published: April 2022

Abstract
Background & Aims: The new metabolic dysfunction-associated fatty liver disease (MAFLD) criteria include three distinct subtypes: MAFLD with diabetes mellitus (DM), overweight/obese (OW), or lean/normal weight with metabolic dysfunction. We investigated whether long-term cardiovascular disease outcomes differ across the MAFLD subtypes.Methods: From a nationwide health screening database, we included 8,412,730 participants (48.6% male) aged 40-64 years, free of cardiovascular disease history, between 2009 and 2010. Participants were categorized into non-MAFLD, OW-MAFLD, lean-MAFLD, and DM-MAFLD. The primary outcome was a composite cardiovascular disease event, including myocardial infarction, ischemic stroke, heart failure, or cardiovascular disease-related death. The presence of advanced liver fibrosis was estimated using a BARD score ≥2. Results: Among the study participants, 3,087,640 (36.7%) had MAFLD, among which 2,424,086 (78.5%), 170,761 (5.5%), and 492,793 (16.0%) had OW-MAFLD, lean-MAFLD, and DM-MAFLD, respectively. Over a median follow-up period of 10.0 years, 169,433 new cardiovascular disease events occurred. With the non-MAFLD group as reference, multivariable-adjusted hazard ratios (95% confidence intervals) for cardiovascular disease events were 1.16 (1.15-1.18), 1.23 (1.20-1.27), and 1.82 (1.80-1.85) in the OW-MAFLD, lean-MAFLD, and DM-MAFLD groups, respectively. Participants with lean-MAFLD or DM-MAFLD had a higher cardiovascular disease risk than those with OW-MAFLD, irrespective of metabolic abnormalities or comorbidities. The presence of advanced liver fibrosis was significantly associated with a higher cardiovascular disease risk in each MAFLD subtype.Conclusion: Long-term cardiovascular disease outcomes differed across the MAFLD subtypes. Further studies are required to investigate whether preventive or therapeutic interventions should be optimized according to the MAFLD subtypes.
Soluble factors in COVID mRNA Vaccine-Induced Myocarditis Causes Cardiomyoblast Hypertrophy and Cell injury: A Case Report
Authors: Paredes-Vazquez Jose Gildardo , Rubio-Infante Nestor , Garza Hector Lopez-de la , Brunck Marion E G , Guajardo-Lozano Jaime Alberto , Ramos Martin R , Vazquez-Garza Eduardo , Torre-Amione Guillermo , Garcia-Rivas Gerardo , Jerjes-Sanchez Carlos 
Published: April 2022

Abstract
Background: Inflammation affecting the heart and surrounding tissues is a clinical condition recently reported following COVID mRNA vaccination. Assessing trends of these events related to immunization will improve vaccine safety surveillance and best practices for forthcoming vaccine campaigns. However, the causality is unknown, and the mechanisms associated with cardiac myocarditis are not understood. Case presentation: After the first dose, we reported an mRNA vaccine-induced perimyocarditis in a young patient with a history of recurrent myocardial inflammation episodes and progressive loss of cardiac performance. We tested this possible inflammatory cytokine-mediated cardiotoxicity after vaccination in the acute phase, and we found a significant elevation of MCP-1, IL-18, and IL-8 inflammatory mediators. Still, at the recovery phase, these cytokines decreased considerably. We used cardiomyoblast in culture to test the effect of serum on cell viability, observing that serum from the acute phase reduced the cell viability to 75%. We did not detect this cellular injury in the sera from the patient in the recovery phase. We also tested serum-induced hypertrophy, a phenomenon presented in myocarditis, and heart failure. We found that acute phase-serum has hypertrophy effects, increasing 25% of the treated cardiac cells' surface and significantly increasing B-type natriuretic peptide. However, we did not observe the hypertrophic effect in the recovery phase or sera from healthy controls. Conclusion: Our results opened the possibility of the inflammatory cytokine or serum soluble mediators-mediated toxicity associated effects. In this regard, identifying anti-inflammatory compounds that reduce inflammatory cytokines could be helpful to avoid vaccine-induced myocardial inflammation.
RNF185 is required for K27-linked polyubiquitination to downregulate Ebola virus glycoprotein GP1,2 expression by reticulophagy
Authors: Zhang Jing , Wang Bin , Johnson Silas , Richard Schwartz , Zheng Yong-Hui 
Published: April 2022

Abstract
Reticulophagy reduces Zaire Ebolavirus (EBOV) glycoprotein GP1,2 expression, and thus increases viral fitness. Reticulophagy degrades endoplasmic reticulum (ER) subdomains containing misfolded proteins to restore the ER proteostasis, but whether degraded cargo proteins are polyubiquitinated is still unknown. Here, we show that the calnexin-calreticulin cycle promoted EBOV-GP1,2 misfolding via PDIA3/ERp57, and that misfolded GP1,2 was degraded via reticulophagy dependent on ATG3, ATG5, and SQSTM1/p62. EBOV-GP1,2 degradation also required its lysine 673 (K673), which served as a substrate for K27-linked ubiquitination. Although E3 ubiquitin ligases RNF26, RNF185, MARCH8, and TRIM25 either degraded or polyubiquitinated EBOV-GP1,2, only RNF185 had both activities. Thus, RNF185 polyubiquitinates misfolded EBOV-GP1,2 in the ER and then SQSTM1/p62 recruits polyubiquitinated GP1,2 into autophagosomes for autophagic degradation. We conclude that ubiquitin is required for reticulophagy, which has played a major role in other autophagic pathways. In addition, EBOV infection should be blocked by enhanced GP1,2 degradation via reticulophagy.
Endoscopic Surgery Versus Drainage of Hematoma under Robot CAS-R-2 for Treatment of Basal Ganglia Hemorrhage
Authors: Zou Dongdong , Chen Xin , Zhang Pengqi , Lu Youming , Zhang Aijun , Yuan Lei , Wei Fance 
Published: April 2022

Abstract
Aims: To compare Endoscopic Surgery and Robot CAS-R-2 drainage for surgical treatment of basal ganglia hypertensive intracerebral hemorrhage. Methods: Fifty consecutive basal ganglia hemorrhage patients were reviewed retrospectively. The primary and secondary outcomes were 6-months mortality and 6-months modified Rankin Scale score, which were assessed by a multivariate logistic regression mode.Results: Compared with the Robot CAS-R-2 group, the 6-month prognosis was significantly higher in the endoscopic evacuation group (OR 5.912, 95% CI 1.014-34.459). Preoperative hematoma volume (OR = 0.931, 95% CI 0.875-0.990) and IVH (OR = 0.578, 95% CI0.396-0.844) were independent predictors for 6-month prognosis. No significant difference in the mortality was found in the two groups (OR 0.501, 95% CI 0.192-1.308).Conclusion: Endoscopic hematoma removal significantly improved the 6-month prognosis compared with the drainage of hematoma under Robot CAS-R-2.
Formation of synthetic RNA protein granules using engineered phage-coat-protein -RNA complexes
Authors: Granik Naor , Katz Noa , Willinger Or , Goldberg Sarah , Amit Roee 
Published: April 2022

Abstract
Liquid-solid transition, also known as gelation, is a specific form of phase separation in which the interacting molecules cross-link to form a highly interconnected compartment with solid – like dynamical properties. Here, we utilize RNA hairpin coat-protein binding sites to form synthetic RNA based gel-like granules via liquid-solid phase transition. we show both in-vitro and in-vivo that hairpin containing synthetic long non-coding RNA (slncRNA) molecules granulate into bright localized puncta. We further demonstrate that upon introduction of the coat-proteins, less-condensed gel-like granules form with the RNA creating an outer shell with the proteins mostly present inside the granule. Moreover, by tracking puncta fluorescence signals over time, we detected addition or shedding events of slncRNA-CP nucleoprotein complexes. Consequently, our granules constitute a genetically encoded storage compartment for protein and RNA with a programmable controlled release profile that is determined by the number of hairpins encoded into the RNA. Our findings have important implications for both the potential regulatory role that naturally occurring granules play and for the broader biotechnology and gene-expression sectors.
Interventions supporting cancer patients in making decisions regarding participation in clinical trials - a systematic review
Authors: Hillersdal Line , Nielsen Zandra Engelbak , Nørmark Ane Taudorf , Knop Ann Soeegaard , Piil Karin 
Published: April 2022

Abstract
Objective: Existing research on the perspectives of patients with cancer and health care professionals indicates that patient decision making on cancer clinical trial participation is a complex process and that Patients’ reasoning may be poorly understood, possibly compromising their decision to participate. This systematic narrative review describes interventions that support patients in their decision to participate and assesses the qualities of the interventions, measures used and related outcomes. Methods: Six databases were systematically searched and only studies evaluating interventions that support the decision making of adult patients with cancer to enter a cancer clinical trial were included. Ten articles met the criteria. Results: The research focus of the included studies reflected the multifactorial nature of what constitutes support for patient decision making in terms of entering a cancer clinical trial. However, most interventions were based on the hypothesis that more information leads to support in decision making, and did not take other factors, such as the relationship to the clinical staff or relatives, the patients’ strong hope for therapeutic benefit or other existential needs into account. The interventions were primarily based on a specific tool, executed once, which seems to imply that decisions need only to be supported once and not at several time points throughout the decision process, and did not assess the importance of a patient’s family- or social relations. Moreover, few interventions focused on the patients’ counselling experience or assessed patient preferences in relation to decision making. Conclusions: The findings show a lack of research on interventions to support patients’ decision making that takes other factors, apart from improving knowledge of trials, into account, such as patients' relationship to the clinical staff or relatives, patients’ strong hope for therapeutic benefit or other existential needs. Limited evidence exists on the effectiveness of decision support interventions to improve the experience of support in adult patients with cancer. Interventions that take the social context of decision processes into account need to be developed and assessed.
Comparison of canine owner profile according to food choice: An online preliminary survey in France.
Authors: Hoummady sara , Fantinati Marco , Maso Djerene , Bynens Aurélie , Banuls Damien , Santos Natalia Ribeiro Dos , Roche Mélanie , Priymenko Nathalie 
Published: April 2022

Abstract
Background
Nowadays, more people are treating dogs as family members. This reflects their increased attention towards their nutrition, with renewed interest for non-conventional diets such as Biologically Appropriate Raw Food/ Bones and Raw Food in United States (BARF) or homemade. In previous studies, owners feeding their dog non-conventional diets reported lower levels of trust in veterinary advice. The aim of the study was to identify differences in lifestyle between owners feeding dogs non-conventional diets and those feeding conventional diets (i.e., dry/wet pet food) to give further insight for improving communication between veterinarians and owners.
Results
A total of 426 surveys were usable. Fifteen percent of the participants lived in the metropole of Paris and had more than one dog (mean 1.72 dogs). Thirty-eight percent of the survey respondents stated that their dogs were fed exclusively with non-conventional diets, while 55% declared using conventional diets alone (not considering treats). The study canine population was for the most part neutered (63%) and purebred (68%). Amongst owners feeding conventional diets exclusively, 47% determined how much food to feed by consulting the feeding guidelines on the packaging, and only 28% said that the amount of food was prescribed by their veterinarian or veterinary nurse. Out of the participants feeding non-conventional diets, 65% declared that the information for formulating the recipes was gathered on the internet or in non-veterinary books. When compared with owners feeding exclusively conventional diets, those feeding non-conventional diets were living more frequently outside the metropole of Paris, had fewer children (0.23 ± 0.57 vs 0.37 ± 0.78; p = 0.03) and had more frequently other animals. They also dewormed less often their pets, walked their dog more each day (91 vs 78%; p < 0.001) and without leash for more than 6 h per week (46 vs 31%; p = 0.003).
Conclusions
This survey described differences in the habits of owners feeding dogs non-conventional diets in comparison with those feeding conventional diets. Data suggest that owners using non-conventional diets may be more attentive to the ethological needs of their dog which could be a starting point for practitioners for achieving better client-veterinarian communication.