bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of an endoplasmic reticulum stress-related signature with potential implications in prognosis and immunotherapy in head and neck squamous cell carcinoma
Authors: Fan Xinlong , Yang Xiao , Guo Nan , Gao Xin , Zhao Yuejiao 
Published: May 2022

Abstract
Background: Head and neck squamous cell carcinoma (HNSCC) is a multisite malignancy which is respond well to immunotherapy. Despite the initial enthusiasm, the clinical benefits of immunotherapy in HNSCC patients are overall limited. Endoplasmic reticulum stress (ERS) has been indicated to play a key role in the process of anti-tumor immune response mediation. However, ERS-related biomarkers which can accurately predict prognosis and immunotherapy response in HNSCC are still lacking. Methods and Results: In this study, we identify and validate an ERS-related signature comprises of six genes (ASNS, EXOSC6, BAK1, TPP1, EXOSC8 and TATDN2) that can predict prognosis of HNSCC patients. GSEA analysis indicates that the ERS-related signature is significantly correlated with tumor immunity in HNSCC. Moreover, the infiltrations of naive B cells and CD8+ T cells are significantly diminished in patients with high-risk scores compared to those with low-risk scores, while macrophages and activated mast cells are remarkably enhanced. Furthermore, the ERS-related signature also displays a tremendous potential for predicting immunotherapy response in HNSCC. Conclusions: Our study identify an ERS-related signature that can predict prognosis of HNSCC patients and highlights its potential value as a predictive biomarker of immunotherapy response, potentially enabling more precise and personalized immunotherapy response and paving the way for further investigation of the prognostic and therapeutic potentials of ERS.
Identification and validation of a novel LncRNA TRG-AS1 that inhibits colorectal cancer proliferation and glycolysis
Authors: Li Lu , Zhang Yong , Li Kunkun , Xing Mengxuan , Xiao Xingguo , Zhang Li , Chu Feifei , Wu Huili 
Published: May 2022

Abstract
Background
Glucose metabolism reprogramming is one of the major hallmarks of malignant tumors and is regulated by long non-coding RNA (LncRNA). However, the role of glycolysis-associated LncRNA in colorectal cancer (CRC) remains largely unknown. In this study, we identified a novel LncRNA TRG-AS1 that inhibits CRC glycolysis and proliferation through comprehensive bioinformatics analysis and experimental validation.
Methods
TRG-AS1 overexpressing stable cell line and silence the target gene cell line were generated. Glucose assay kits and Pyruvate assay kits were utilized to analyze glycolysis. The cell proliferation ability was evaluated by CCK8, Colony formation assays and EdU. Xenograft tumor experiments were performed to assess proliferative capacity. Cell migration and invasion abilities were examined using the Transwell assay.
Results
TRG-AS1 was significantly down-regulated in CRC tumors as revealed by nine independent CRC cohorts, including our own in-house cohort. Clinically, high expression of TRG-AS1 predicts favorable overall survival (OS) and disease-free survival (DFS) in CRC patients, and high level of TRG-AS1 is closely associated with earlier tumor stage. In terms of function, over-expression of TRG-AS1 significantly inhibited CRC aerobic glycolysis, proliferation, metastasis potential and tumor growth, while knockdown of TGR-AS1 dramatically promoted CRC aerobic glycolysis and progression. Data based on bioinformatics analysis suggest that TRG-AS1 may be involved in CRC progression by influencing glycolysis-related molecules, such as SLC2A1, PGK1 and ENO1, etc.
Conclusion
Our study advances the understanding that LncRNA inhibits CRC glycolysis and progression, and provide a promising molecular target for the treatment of CRC.
Antibody-dependent cellular cytotoxicity-null effector developed using mammalian and plant GlycoDelete platform
Authors: Kang Cho Eun , Lee Seungeun , Ahn Taeyoung , Seo Dong Hye , Ko Byung Jun , Jung Minkyu , Lee Jinu , Kim Joo Young , Kim Woo Taek 
Published: May 2022

Abstract
Cancer therapy using immune checkpoint inhibitor antibodies has markedly shifted the paradigm of cancer treatment. However, methods completely eliminating the immunogenicity of these signal-regulating antibodies is urgently required. The heterogeneity of glycan chains in antibodies limits their use as therapeutic agents due to their variability; thus, the development of uniform glycan chains is necessary. Here, we subjected the anti-programmed cell death protein (PD)-1 antibody nivolumab, a representative immune checkpoint inhibitor, to GlycoDelete (GD) engineering to remove the antibody-dependent cellular cytotoxicity (ADCC) of the antibody, leaving only one glycan in the Fc. Glyco-engineered CHO cells were prepared by overexpressing endo-β-N-acetyl-glucosaminidase in CHO cells, in which N-acetyl-glucosaminyl-transferase I was knocked out using Cas9. GD IgG1 nivolumab and GD IgG4 nivolumab were produced using GD CHO cells, and glycan removal was confirmed using mass spectrometry. Target binding and PD-1 inhibition was not altered; however, ADCC decreased. Furthermore, the IgG4 form, determined to be the most suitable form of GD nivolumab, was produced in a plant GD system. The plant GD nivolumab also reduced ADCC without affecting PD-1 inhibitory function. Thus, CHO and plant GD platforms can be used to improve signal-regulating antibodies by reducing their immunogenicity.
Compliance and outcome of osteoporosis treatment after total knee arthroplasty
Authors: Yoon Jung-Ro , Koh Yeong Yoon , Lee Seung Hoon 
Published: May 2022

Abstract
Background: Treatment compliance is important in the treatment of osteoporosis. Our hypotheses were as follows: 1) compliance with osteoporosis treatment after total knee arthroplasty (TKA) will be high, and 2) osteoporosis treatment results after TKA will be as good as those of patients who do not undergo TKA.Methods and Materials: Patients diagnosed with osteoporosis for the first time in an outpatient clinic (OPD group) and those who were diagnosed at hospitalization for TKA (TKA group) from March 2019 to December 2020 were retrospectively evaluated. Only patients treated with denoxumab were included in the study. Compliance, based on the outpatient follow-up rate after 1 year, was compared between the two groups, and the treatment outcomes were compared based on bone mineral density (BMD). Results: In the OPD group, 29 of 41 patients were followed up 1 year later, and in the TKA group, all 14 patients were followed up after 1 year. In the OPD group, the BMD increased significantly. In the TKA group, there was a significant increase in BMD only in the lumbar spine. However, there were no significant between-group differences in the BMD before and after treatment. Conclusion: Osteoporosis treatment compliance was significantly higher in the TKA group than in the OPD group. When denoxumab was used after TKA, there was no decrease in BMD, and osteoporosis treatment results after TKA were similar to those of the OPD group.
Cellulose Grafted with Polyaniline for Simultaneous Adsorption of Cationic and Anionic Dyes in Wastewater Effluent
Authors: Nguyen Thu Ha , Nguyen Manh Trung , Vuong Bich Hien , Le Trong Huyen 
Published: May 2022

Abstract
The adsorbent for both cationic and anionic dye based on cellulose and polyaniline was prepared. Cellulose was purified from sugarcane bagasse through removal of hemicellulose and lignin. Aniline was polymerized in emulsion state in the presence of cellulose and ammonium persulfate as initiator. The FTIR spectroscopy, SEM observation and determination of porosity confirmed that polyaniline was grafted on the surface of cellulose fiber to form cellulose-graft-polyaniline with high porosity. Polyaniline acted as adsorption sites for both cationic dye (methylene blue) and anionic dye (methyl orange). The adsorption on the surface of cellulose-graft-polyaniline followed the Langmuir model, suggesting that the adsorption sites on its surface was uniform. The extent of adsorbing capacity of cellulose-graft-polyaniline toward methylene blue and methyl orange was 1.57 and 0.75 mmol/g, respectively. These values confirmed that cellulose-graft-polyaniline was an efficient adsorbent for both cationic and anionic dyes. The adsorption of either cationic or anionic dye in a mixture solution did not restrain each other. Cellulose-graft-polyaniline was expected to have potential application in practical.
Follow-up of neonatal screening using automatic auditory brainstem responses in infants with congenital syphilis: prospective cross-sectional study
Authors: Aguiar Livia Barbosa , Cunha Brenda Karla Silva da , Bezerra Mylena Taíse Azevedo Lima , de Brito Maria Edinilma Felinto , Arrais Nívia Maria Rodrigues , Moore David R , Balen Sheila Andreoli 
Published: May 2022

Abstract
Objective: The risk of congenital syphilis for neonatal hearing loss is currently unclear. In this study we determined the occurrence of risk for hearing loss during follow-up of neonatal hearing screening in infants with congenital syphilis during the first two months of life. Methodology: Prospective cross-sectional study. Automatic auditory brainstem response (AABR) was performed after hospital discharge (nine to 60 days). The sample consisted of 93 newborns in public maternity hospitals, divided into three groups: Exposed to syphilis: seven newborns of mothers who underwent the current best practice treatment for syphilis during the prenatal period after detecting positive for the disease using the Venereal diseases research laboratory (VDRL) exam; Congenital syphilis: 37 newborns and their mothers who received syphilis treatment during the perinatal period, following a positive result of VDRL at birth; Control: 49 newborns of women who had a negative result of VDRL, showing absence of syphilis during pre- and perinatal periods. None of the newborns had other risk indicators for hearing loss. Analysis was based on binary pass/failure in AABR, the standard clinical practice. Results: In the Exposed group, 7/7 newborns (100%) passed the test bilaterally. In the Congenital group, 36/37 (97%) passed the test for the right ear and 35/37 (94%) for the left ear. In the Control group, 47/49 (96%) passed the test for the RE and 46/49 (94%) for the left ear. The proportion of passes did not differ statistically between groups or ears. Conclusion: Neither exposure to syphilis nor the presence of congenital syphilis were indicators of a higher occurrence of neonatal hearing screen failure during the first two months of life.
Brewer’s spent yeast replacement in carp diet leads to muscle biomass production, recycling, waste management and resource conservation
Authors: Pradhan Debashish , Mahanty Arabinda , Mohanty Sasmita , Samantaray Kasturi , Mohanty Bimal Prasanna 
Published: May 2022

Abstract
Brewer’s-spent-yeast (BSY) is among the most voluminous byproducts generated in brewery industry that adds to the waste; however, smart utilization of BSY could lead to edible biomass production besides waste management. To utilize it for biomass production, it is being used in fish feeds; however, its effect on the fish physiology has been scantily studied. The present study investigated the proteomic changes in muscle tissues of carp Labeo rohita fed with BSY-based diet, to understand its impact on muscle physiology and biomass. Six feeds were prepared with different grades of BSY (0, 20, 30, 40, 50, 100% replacement of fishmeal with BSY) and fishes were fed for 90 days. Highest weight gain%, feed conversion efficiency, specific growth rate% were observed in 30% BSY-replaced group and this group was considered for the proteomic study. Comparative shotgun proteomic analysis was carried out by LC-MS/MS and data generated have been deposited in ProteomeXchange Consortium with dataset identifier PXD020093. A total of 62 proteins showed differential abundance; 29 increased and 33 decreased in the 30%BSY replaced group. Pathway analysis using IPA and Panther tools revealed that the proteins tyrosine protein kinase, PDGFα, PKRCB and Collagen promote muscle growth by inducing the PI3K-AKT pathway. Conversely, the proteins Serine/threonine-protein phosphatase, Phosphatidylinositol 3,4,5-trisphosphate5-phosphatase 2A, Ras-specific guanine- nucleotide-releasing factor inhibit muscle growth indicating that 30% BSY replaced feed promote muscle growth in a highly controlled manner. Findings suggest that BSY could be recycled for carp feed production in large-scale thereby leading to resource conservation, reducing environmental effects.
Effect of feeding sulphate reducing bacteria along with sulphur on growth performance, nutrient utilization and methane emission in goats
Authors: Uniyal Sandeep , chaudhary Lal chandra , Kala Anju , Agarwal NEETA , chaturvedi Vishwa bandu 
Published: May 2022

Abstract
Competitive relationship exist between the sulphate reducing bacteria and the methanogens in the anaerobic environment including rumen for hydrogen where sulphate is not limiting for the growth and consequently inhibit the enteric methane emission as thermodynamically energetic of sulphate reduction (∆Go= -21.1 KJ/mole of H2) is more favourable than methanogenesis (∆Go= -16.9 KJ/mole H2). To validate this hypothesis a study was designed to investigate the effect of supplementation of sulphate reducing bacteria (SRB) as microbial feed additives alone or along with sulphur (as sodium sulphate) on methane production, live weight gain, feed intake, nutrient digestibility and energy metabolism in goats. The SRB used as microbial feed additives were isolated from goat rumen. The experiment was conducted on growing kids (n = 36, 5–6 months of age) with average body weight of 10.08 ± 0.21 kg, divided in six groups (n = 6). The six treatments were control fed basal diet (T1), SRB 0.5ml/kg BW (T2), sulphur (as sodium sulphate) 0.095% of DMI (total sulphur level in the diet 1.5 times the requirement) (T3), sulphur (as sodium sulphate) 0.095% of DMI + SRB 0.5ml/kg BW (T4), sulphur (as sodium sulphate) 0.19% of DMI (total sul- phur level in the diet 2 times the requirement) (T5) and sulphur (as sodium sulphate) 0.19% of DMI + SRB 0.5ml/kg BW (T6). Duration of study was 150 days and the goats were fed as per ICAR (2013) feeding standard. Methane (CH4) production (l/kg DMI) was reduced by 11.8% (P = 0.05) in T6 where sulphur (@ 0.19% DMI) was supplemented along with SRB4 (@ 0.5 ml/kg BW) as compared to T1 (un-supplemented group). However, the dry matter intake (DM), total weight gain (TG), average daily gain (ADG), feed conversion ratio (FCR), excretion of purine derivatives (allantoin, uric acid, xanthine and hypoxanthine) and digestibility of organic matter (OM), dry matter (DM), ether extract (EE), crude protein (CP), acid detergent fibre (ADF) and neutral detergent fibre (NDF) remained comparable (P > 0.05) among all the groups. The experimental data revealed that feeding of SRB as an microbial feed additives along with sulphur (as sodium sulphate) is capable in reducing enteric CH4 emission without any adverse effect on rumen fermentation and digestibility of the nutrients.
Positive solution for weighted logarithmic problem involving continuous potential under exponential nonlinearities growth
Authors: DRIDI Brahim , JAIDANE Rached 
Published: May 2022

Abstract
In this paper, we study the nonlinear weighted elliptic problem
[[EQUATION]]
where $B$ is the unit ball of $\mathbb{R^{N}}$, $N>2$, $ w(x)=\big(\log \frac{1}{|x|}\big)^{\beta(N-1)}$,$~~\beta\in[0,1)$
the singular logarithm weight with the limiting exponent $N-1$ in the Trudinger-Moser embedding. $V$ is a continuous positive potentiel.
The nonlinearities are critical or subcritical growth in view of Trudinger-Moser
inequalities. We prove the existence of non trivial
solutions via the critical point theory. In the critical case, the associated energy functional does not satisfy
the compactness condition. We give a new growth condition and we point out its importance
for checking the Palais-Smale compactness condition.
Strontium (II) biosorption studies on starch-functionalized magnetic nanobiocomposites using full factorial design method
Authors: Prill Busra , Sedir Umitcan , Yusan Sabriye 
Published: May 2022

Abstract
The purpose of this research was to fabricate starch based magnetic nanobiocomposites using 2 different starch types (corn and wheat) and to examine the effects of some experimental parameters (such as starch concentration, NaOH concentration and aging time of nanoparticles) that play a role in the synthesis products. The characterization of the starch-based magnetic-nanobiocomposite materials were realized with several techniques. The usability of starch-based magnetic-nanobiocomposite materials, which can provide rapid separation with their magnetic properties and are not toxic, in removing Sr (II) ions from aqueous solutions has been investigated. The parameters affecting the biosorption were investigated using a full factorial experimental design. In the biosorption study, pH, temperature, initial Sr(II) concentration and contact time were determined as four independent variables. The regression coefficients were found using the least squares method and the response surface graphs were created according to the polynomial equation obtained from the full factorial experimental design. ANOVA (analysis of variance) analysis within the 95% confidence interval of the model applied for the full factorial experimental design was examined and the compatibility of the model with the experimental findings was examine. The composition and chemical state of the magnetic nanobiocomposites were investigated by XPS analysis after Sr(II) biosorption. For the purpose of determine the adsorption model, the relevant parameters were calculated using Langmuir, Freundlich and Dubinin-Radushkevich isotherms. Gibbs free energy change, enthalpy and entropy values, which are the values of adsorption thermodynamics, were calculated.
Mechanical activation of mitochondrial energy metabolism during cell differentiation
Authors: Wang Zong-Heng , Combs Christian , Zhao Wenjing , Knutson Jay , Lilly Mary , Xu Hong 
Published: May 2022

Abstract
In multicellular lives, differentiation of many types of stem and progenitor cells is often accompanied by a metabolic transition from glycolysis to mitochondrial oxidative phosphorylation. The mechanisms driving this metabolic transition in vivo are largely unknown. Here, we show that, during differentiation of the Drosophila female germline cyst, the surrounding somatic cells compress the cyst and increase the tension of cyst cells’ membranes. Transmembrane channel-like, an evolutionarily conserved ion channel involved in mechanosensation, maintains cytosolic Ca2+ levels in compressed differentiating cysts. Cytosolic Ca2+ induces transcriptional activation of oxidative phosphorylation through a CaMKI-Fray-JNK signaling relay. Our findings demonstrate a molecular link between cell mechanics and mitochondrial energy metabolism, with implications in other developmentally orchestrated metabolic transitions in mammals.
Clinician Reports of Self-Awareness after Traumatic Brain Injury: A Retrospective Chart Review
Authors: Mamman Rinni , Cheng Anika , Tsow Rebecca , Schmidt Julia 
Published: May 2022

Abstract
Background: Impaired self-awareness (i.e., lack of insight) is experienced by most individuals after a moderate to severe traumatic brain injury (TBI). These individuals do not recognize their abilities and limitations which can negatively impact daily life and function. Although there are evidence-based approaches to improve self-awareness after TBI, it is not known how clinicians respond and address this impairment in an inpatient rehabilitation setting.Objective: To examine how clinicians report, assess, and provide intervention for impaired self-awareness after TBI.Methods: A retrospective chart review was conducted on interdisciplinary rehabilitation clinician entries for individuals with TBI (n=67) who received inpatient rehabilitation within a five-year period (2014-2019). A reflexive thematic analysis approach was used to analyse the data.Results: Three themes were generated to explore clinician responses to their clients’ impaired self-awareness: 1) ‘recalling and understanding’ described clinicians observing client behaviours and expressions of self-awareness, 2) ‘applying and analyzing’ identified clinicians providing relevant tasks and advice to clients, and 3) ‘evaluating and creating’ described clinicians actively interacting with clients by providing feedback, guided prompts, and a follow-up plan. Conclusion: Clinicians described varied responses to clients’ impaired self-awareness after TBI. Findings may help to develop research priorities and integrated knowledge translation initiatives to increase evidence-based practice for impaired self-awareness after TBI.
The Effect of Topical Ketamine Administration on the Corneal Epithelium Repair
Authors: Sanatkar Mehdi , Nozarian Zohre , Bazvand Fatemeh , Abdi Parisa 
Published: May 2022

Abstract
Introduction: The cornea is regarded as a sensitive organ to pain. Ketamine can effectively reduce postoperative neuropathic pain. We hypothesized that topical ketamine could mitigate postoperative corneal neuropathic pain. The aim of this study was to determine whether topical ketamine is safe for cornea and evaluate its effect on the repair procedure the damaged corneal tissue.Methods: Our study was performed on only the right eyes of 15 male rats. All animals underwent general anesthesia and the whole corneal epithelium was removed. All subjects were divided into two groups: group 1 (n = 8), one drop of ketamine, and group 2 (n = 7), one drop of 0.9% sodium chloride administered topically on the scraped cornea every 6 hours for seven days. The rats’ s cornea was carefully monitored daily for the size of epithelial defects under a microscope and was photographed. On the eighth day, the eyes were sent for pathological examination. The eyes were examined for the amount of inflammation, neovascularization, keratinization, epithelial thickness and Descemet's membrane pathologies.Results: The epithelial defect has healed completely on the sixth day in all rats in both groups. There was no significant difference in the speed of complete recovery between the two groups. No significant difference was observed between the two groups in terms of inflammation grade, neovascularization grade, and epithelial thickness.Conclusion: Our study showed that topical ketamine had no significant effect on corneal wound healing in a rat animal model and could be used safely for the management of postoperative chronic ocular pain.
Tunable reverse rectification of layed Janus MSeS (M=Hf, Zr) and SnS2 heterojunctions
Authors: Pan Jinghua , Jing Sicheng , Chen Wen , Li Wei , Wang Yu , Bian Baoan , Biao Liao , Wang Guoliang 
Published: May 2022

Abstract
Two-dimensional (2D) Janus transition metal dichalcogenides (JTMDs) exhibit suitable band gaps and strong visible-light absorption, which are extensively applied to the field of optoelectronic devices. Here, we investigate the electronic properties of 2D JTMDs MSeS (M=Hf, Zr) and SnS2 van der Waals heterojunction through density functional theory. The calculated electronic properties reveal that ZrSeS/SnS2 heterojunction has a type-I band alignment, while HfSeS/SnS2 heterojunction has a type-II band alignment. We build the diodes based on the MSeS (M=Hf, Zr)/SnS2 heterojunctions and study the electronic transport. The currents of the devices exhibit the asymmetry and the negative turn-on voltages suggest that constructed devices are backward diodes. Moreover, it is found that the gate voltage can modulate the rectifying ratio, and the rectifying performance of ZrSeS/SnS2 is better than that of HfSeS/SnS2.