bluuebunny/crossref_metadata_2025_split huggingface dataset:
PREDICT validity for prognosis of breast cancer patients with pathogenic BRCA1/2 variants
Authors: Muranen Taru , Morra Anna , Khan Sofia , Barnes Daniel , Bolla Manjeet , Dennis Joe , Keeman Renske , Leslie Goska , Parsons Michael , Wang Qin , Ahearn Thomas , Aittomäki Kristiina , Andrulis Irene , Arun Banu , Behrens Sabine , Białkowska Katarzyna , Bojesen Stig , Camp Nicola , Chang-Claude Jenny , Czene Kamila , Devilee Peter , Domchek Susan , Dunning Alison , Engel Christoph , Evans Gareth , Gago-Dominguez Manuela , Garcia-Closas Montserrat , Gerdes Anne-Marie , Glendon Gord , Guénel Pascal , Hahnen Eric , Hamann Prof U. , Hanson Helen , Hooning Maartje , Hoppe Reiner , Izatt Louise , Jakubowska Anna , James Paul , Kristensen Vessela , Lalloo Fiona , Lindeman Geoffrey , Mannermaa Arto , Margolin Sara , Neuhausen Susan , Newman William , Peterlongo Paolo , Phillips Kelly-Anne , Pujana Miquel Angel , Rantala Johann , Rønlund Karina , Saloustros Emmanouil , Schmutzler Rita , schneeweiss andreas , Singer Christian , Suvanto Maija , Tan Yen Yen , Teixeira Manuel , Thomassen Mads , Tischkowitz Marc , Tripathi Vishakha , Wappenschmidt Barbara , Zhao Emily , Easton Douglas , Antoniou Antonis , Chenevix-Trench Georgia , Pharoah Paul , Schmidt Marjanka , Blomqvist Carl , Nevanlinna Heli 
Published: November 2022

Abstract
We assessed the PREDICT v 2.2 for prognosis of breast cancer patients with pathogenic germline BRCA1 and BRCA2 variants, using follow-up data from 5453 BRCA1/2 carriers from the Consortium of Investigators of Modifiers of BRCA1/2 (CIMBA) and the Breast Cancer Association Consortium (BCAC).
PREDICT for estrogen receptor (ER)-negative breast cancer had modest discrimination for BRCA1 carrier patients overall (Gönen & Heller unbiased concordance 0.65 in CIMBA, 0.64 in BCAC), but it distinguished clearly the high-mortality group from lower risk categories. In an analysis of low to high risk categories by PREDICT score percentiles, the observed mortality was consistently lower than the expected mortality, but the confidence intervals always included the calibration slope. Altogether, our results encourage the use of the PREDICT ER-negative model in management of breast cancer patients with germline BRCA1 variants. For the PREDICT ER-positive model, the discrimination was slightly lower in BRCA2 variant carriers (concordance 0.60 in CIMBA, 0.65 in BCAC). Especially, inclusion of the tumor grade distorted the prognostic estimates. The breast cancer mortality of BRCA2 carriers was under-estimated at the low end of the PREDICT score distribution, whereas at the high end, the mortality was over-estimated. These data suggest that BRCA2 status should also be taken into consideration with tumor characteristics, when estimating the prognosis of ER-positive breast cancer patients.
Health risk assessment of heavy metal contamination of groundwater around Nnewi Industrial Area, Anambra State, Nigeria
Authors: Eboagu N. C. , Ajiwe V. I.E. 
Published: November 2022

Abstract

The influence of water quality from boreholes situated around the Nnewi industrial area was evaluated by assessing the heavy metal concentrations and health risks associated with human exposure. Water samples were collected from 16 different boreholes around eight industries at one month interval from May – October 2019 and November 2019 – April 2020 to cover the rainy and dry seasons, respectively. Water samples were analyzed for copper (Cu), lead (Pb), iron(Fe), zinc(Zn), chromium (Cr), arsenic(As), cadmium(Cd) and nickel(Ni) using atomic absorption spectrophotometer (AAS). The results were compared with the World Health Organization (WHO) standards. Data obtained for both seasons indicate Cu ranges of (0.04–0.81) mg/L, Pb (0.00–0.09) mg/L, Fe (0.00–0.83) mg/L, Zn (0.26–1.48) mg/L, Cr (0.01–0.09) mg/L, and As(0.01–0.02) mg/L, respectively. All the heavy metal concentrations of the sampled water were within their various WHO permissible limits except As, Cr and Pb. The pollution index of As, Cr and Pb were greater than 1, showing heavy contamination of the water. Carcinogenic risk assessment of water via ingestion and dermal route for Cr and As were above the recommended safe limit of 1×10− 6which posed a cancer risk. The results show that borehole waters were contaminated with heavy metals in both seasons. It is, therefore, recommended that borehole water from the study areas should be treated before used for various domestic purposes.
Barriers and enablers of pelvic floor rehabilitation behaviours in pregnant women with stress urinary incontinence: A qualitative analysis using the Theoretical Domains Framework
Authors: Xu Ping , Jin Ying , Guo Pingping , Xu Xuefen , Wang Xiaojuan , Zhang Wei , Mao Minna , Feng Suwen 
Published: November 2022

Abstract
Background‎
Stress urinary incontinence during pregnancy is closely related to the occurrence of postpartum and long-term urinary incontinence. Early pelvic floor management is of great significance in promoting the recovery of pelvic floor tissues in pregnant women. However, effective management of urinary incontinence is far from achievable owing to the low compliance of pregnant women in partaking in pelvic floor rehabilitation. In this study, we aimed to identify barriers and enablers of pelvic floor rehabilitation behaviours in pregnant women with stress urinary incontinence.
Methods‎
A descriptive, qualitative design was used in this study. Face-to-face semi-structured interviews were conducted with pregnant women with stress urinary incontinence based on the Theoretical Domain Framework. The data were analysed using a combination of inductive and deductive methods. The study followed the COREQ guidelines.
Results
Twenty pregnant women with stress urinary incontinence were interviewed. Seven themes were summarised and used to explain the pelvic floor rehabilitation behaviours of pregnant women with stress urinary incontinence. The seven themes were (1) individual knowledge and experience of pelvic floor management, (2) judgments about expected outcomes, (3) interactions of interpersonal situations, (4) environment, resources, and decision-making processes, (5) personal goal-setting and efforts towards behaviour change, (6) emotional influences on decision-making, and (7) personality traits. After deductive mapping of topics to the Theoretical Domains Framework, it was found that 13 of the Theoretical Domains Framework domains affected the pelvic floor management behaviours of pregnant patients.
Conclusions
The pelvic floor rehabilitation behaviours of pregnant women with stress urinary incontinence are complex and are affected by many factors. Understanding how pregnant patients feel about pelvic floor rehabilitation behaviours and what factors influence their decision to adopt them can guide healthcare professionals in providing targeted interventions to improve adherence to pelvic floor rehabilitation behaviours.
Structural Assessment of the Full-Length Wild-Type Tumor Suppressor Protein p53 by Mass Spectrometry-Guided Computational Modeling
Authors: Sinz Andrea , Ianni Alessio Di , Ihling Christian , Kipping Marc , Köppen Janett , Arlt Christian , Iacobucci Claudio , Tüting Christian , Kastritis Panagiotis 
Published: November 2022

Abstract
The tetrameric tumor suppressor p53 represents a great challenge for 3D-structural analysis due to its high degree of intrinsic disorder (ca. 40%). We aim to shed light on the structural and functional roles of p53’s C-terminal region in full-length, wild-type human p53 tetramer and their importance for DNA binding. For this, we employed complementary techniques of structural mass spectrometry (MS) in an integrated approach with AI-based computational modeling. Our results show no major conformational differences in p53 between DNA-bound and DNA-free states, but reveal a substantial compaction of p53’s C-terminal region. This supports the proposed mechanism of unspecific DNA binding to the C-terminal region of p53 prior to transcription initiation by specific DNA binding to the core domain of p53. The synergies between complementary structural MS techniques and computational modeling as pursued in our integrative approach is envisioned to serve as general strategy for studying intrinsically disordered proteins (IDPs) and intrinsically disordered region (IDRs).
The biological and prognostic role of long non-coding RNA NEAT1 in acute myeloid leukemia
Authors: Miliara Sophia , Neddermeyer Anne , Bonetti Alessandro , Shin Jay W. , Mujahed Huthayfa , Carninci Piero , Arner Erik , Soren Lehmann , Lennartsson Andreas 
Published: November 2022

Abstract
Nuclear paraspeckle assembly transcript 1 (NEAT1) is a long non-coding RNA associated with the promotion of several solid cancers. As part of the FANTOM6 project, we aimed to define its role in acute myeloid leukemia (AML) and myelopoiesis, since it remains largely uninvestigated. We show that NEAT1 expression increases during myelopoiesis, especially in monocytes. NEAT1 expression is elevated in AML compared to normal bone marrow (NBM), especially in AML with inv(16)and t(8;21). In addition, NEAT1 expression correlates positively with ASXL1, KRAS and NRAS mutations, but negatively with TP53 mutant AML. Higher NEAT1 expression is associated with better overall survival in AML, independent of other known risk factors. Knockdown of NEAT1 in AML cells induces monocytic differentiation and upregulated gene expression of genes involved in glucose metabolism. By investigating genome-wide RNA and DNA interactions using RADICL-sequencing, we show that NEAT1 binds to the RUNX2 locus that is associated with differentiation, metabolism, and glucose uptake. The results suggest that the lncRNA NEAT1 has a role in myelopoiesis, AML and is implicated in glycolysis.
Runx2 Overexpression Promotes Bone repair of Osteonecrosis of the femoral head (ONFH)
Authors: Xu Hai-Jia , Liu Xiangzhong , Yang Lu , Xu Liang-Liang , Sun Da-Ming , Liao Wen , Yang Yi , Li Zhang-Hua 
Published: November 2022

Abstract
Background: Runt-related transcription factor-2 (Runx2) has been considered an inducer to improve bone repair ability of mesenchymal stem cells (MSCs).
Methods and Results: Twenty-four rabbits were randomly devided into four groups. The Ad-Runx2 group was administrated with Runx2 overexpression MSCs, the Runx2-siRNA group was treated with Runx2-siRNA transfection MSCs, the MSCs group was injected with MSCs, and the Model group was treated with saline. The injection was administered at 1 week and 3 weeks after model establishment. The expression of BMP-2, Runx2 and Osterix from the femoral head was detected at 3 and 6 weeks after MSCs being injected, and Masson Trichrome Staining, Gross Morphology, X-ray and CT images Observation were used to evaluate the repair effect of ONFH. The data revealed that the expression of BMP-2, Runx2 and Osterix in the Runx2-siRNA group was reduced at 3 weeks compared with the MSCs group, and then the expression further reduced at 6 weeks, but was still higher than the Model group besides Osterix; The expression of these three genes in the Ad-Runx2 group was higher than in the MSCs group. Masson Trichrome Staining, Gross Morphology and X-ray and CT images Observation revealed that necrotic femoral head of the MSCs group was more regular and smooth than the Runx2-siRNA group, which has a collapsed and irregular femoral head. In the Ad-Runx2 group, necrotic femoral head was basically completely repaired and covered by rich cartilage and bone tissue.
Conclusions: Overexpression of Runx2 can improve osteoblastic phenotype maintenance of MSCs and promote necrotic bone repair of ONFH.
Medication administration errors in the domain of infusion therapy on intensive care units: A survey study among nurses
Authors: BEAUDART Charlotte , WITJES Maureen , ROOD Paul , Hiligsmann Mickael 
Published: November 2022

Abstract
Background : Despite extensive research carried out on medication administration errors (MAEs) in the domain of infusion therapy, there is limited knowledge on nurses perceptions on the occurrence of MAEs during infusion therapy. Since nurses are responsible for medication preparation and administration in Dutch hospitals, it is vital to understand their perspective on the risk factors for MAEs.
Aim: The purpose of this study is investigated the perception of nurses, working on adult ICUs, on the occurrence of MAEs during continuous infusion therapies.
Methods: A digital web based survey was distributed among 91 ICU nurses working in Dutch hospitals. The survey investigated nurses’ perceptions on the frequency, severity of consequences and preventability of MAEs, factors for the occurrence of MAEs, and infusion pump and smart infusion safety technology.
Results: A total of 91 nurses completed the survey Medication-related factors and intensive care-related factors were perceived as the two most important risk categories for the occurrence of MAEs. Important risk factors contributing to the occurrence of MAEs included high patient-nurse ratio, problems in communication between caregivers, frequent staff changes and transfers of care, and no/incorrect dosage/concentration on labels. Drug library was reported as the most infusion pump feature and both Bar Code Medication Administration and medical device connectivity as the two most important smart infusion safety technologies. Nurses perceived the majority of MAEs as preventable.
Conclusions:Results observed in this study suggest ICUs to focus on prevention strategies to reduce risk factors for MAEs as well as to increase awareness among nurses on the risks of MAEs.
Analysis of the Coronary Care Unit Bed Allocation policy and selecting the most suitable model for optimal allocation in a low and middle-income country: a multiphase study
Authors: Headayati Maryam , Azami-Aghdash Saber , Arabloo Jalal , Rezapour Aziz , Alipour Vahid , Moradi Najmeh , Yousefzadeh Negar , Zarei Leila , Zanganeh Marzieh 
Published: November 2022

Abstract
Background
This study aimed to conduct a policy analysis on allocating Coronary Care Unit (CCU) beds and clarifies the well-suited model for allocating CCU beds in Iran.
Methods
As a multiphase study, this was conducted in 4 steps, including a literature review, semi-structured interviews, an analysis of the policymaking process, and the Delphi study. The bed allocation models, their criteria, and the challenges of CCU bed allocation were explored through a literature review and semi-structured interviews. The CCU beds allocation policy was analyzed using Walt and Gilson's Health Policy Triangle (HPT). The Delphi method chose the suitable model for the optimal allocation of CCU beds in Iran.
Results
Findings were reported in four dimensions of the policy analysis triangle, including content, context, process, and actors. Various social, economic, environmental, and political factors affect policy development. Allocating policy emphasizes fair distribution of resources and full access to health services and encourages the private sector. Achieving efficiency of hospital bed utilization put bed allocation on the agenda in Iran. Thorough literature review, 11 models and 14 indicators were found. After conducting interviews, the number of indicators increased to 58 cases, and the CCU bed allocation challenges were identified. The Trend Model was picked through the Delphi study as a framework for CCU bed allocation.
Conclusion
Using the results of the present study and the proposed model can help to allocate hospital beds, especially CCU beds, optimally and efficiently. However, challenges and barriers identified by this study require consideration during the development of the proposed model. Also, managers and policymakers require information about the most effective strategies to reduce the need for CCU beds in the future. It is recommended that future studies and scenario planning be considered prior to the optimal allocation of hospital beds.
Tissue-like cultured fish fillets through a synthetic food pipeline
Authors: Liu Donghong , Xu Enbo , Niu Ruihao , Lao Jihui , Zhang Shengliang , Li Jie , Zhu Yiyuan , Shi Huimin , Zhu Qingqing , Chen Yijian , Jiang Yuyan , Wang Wenjun , Yin Jun , Chen Qihe , Huang Xiao , Chen Jun 
Published: November 2022

Abstract
Tissue-like cultured meats of some livestock have successfully been established by different approaches. However, production of a structure similar to fish fillets is still challenging. Here, we develop tissue-like cultured fish fillets by assembly of large yellow croaker muscle fibers and adipocytes with 3D-printed gel. Inhibition of Tgf-β and Notch signals significantly promoted myogenic differentiation of piscine satellite cells (PSCs). The mixture of fish gelatin and sodium alginate combined with a p53 inhibitor and a Yap activator supported PSC viability and proliferation. Based on the texture of fish muscle tissue, a 3D scaffold was constructed by gelatin-based gel mixed with PSCs. After proliferation and differentiation, the muscle scaffold was filled with cultured piscine adipocytes. Finally, tissue-like fish fillets with 20×12×4 mm were formed, consisting of 5.67×107 muscles and 4.02×107 adipocytes. The biomanufacture of tissue-like cultured fish fillet here could be a promising technology to customize meat production with high fidelity.
Learn from the Best Hospitals: A Comparison of the Mission, Vision and Values
Authors: Qin Xiaoping , Wang Bing-Long , Zhao Jinhong , Wu Peixin , Liu Tingfang 
Published: November 2022

Abstract
Background: The hospital's mission, vision, and values are the core of the hospital's culture and the most profound expression of the hospital's culture. Although there have been many comparative studies on the mission, vision and values of organizations in the past, there have been few studies on the mission, vision and values of hospitals in the healthcare field. The purpose is to understand how the world's top hospitals perform the effective role of mission, vision and value.
Methods: This paper collects and discusses the approaches of the world's top five hospitals in mission, vision and value through a qualitative analysis method. Documents for the study were collected from the publicly available information of the five hospitals, including their websites, annual reports, and relevant academic literature published in English on Google Scholar, PubMed, Medline, and Web of Science.
Results: These five hospitals have similarities and differences in the development of their missions, visions and values, which are worthy of study by other hospitals. The setting of a mission is a useful reflection of the hospital's focus and direction showing the social responsibility and sustainability of the hospital. The development of a vision has a guiding role in the equity and development of patients and employees and can improve the efficiency and effectiveness of hospital management and ensure the quality of services. The elaboration of values can greatly help hospitals to develop strategic plans and improve daily management.
Conclusion: Our research found that the top five hospitals in the world, regardless of the nature of the hospital or their management model, have some overlap in their missions, visions and values. In addition, each hospital also has some distinctive description that reflects their special characteristics.
Targeted therapy monitoring of BRAF-V600-mutant Erdheim-Chester disease by fast quantitative whole-body bone CZT-tomoscintigraphies
Authors: MELKI Saifeddine , Moulinet Thomas , Verger Antoine , Marie Pierre-Yves , Imbert Laetitia , Bahloul Achraf 
Published: November 2022

Abstract
Erdheim-Chester disease (ECD) is a rare histiocytosis due to proto-oncogene mutations, primarily affecting the long bones and possibly being treated by novel targeted therapies. 18F-FDG PET is a reference technique for ECD assessment. However, we present a case where easier and more objective monitoring of the ECD-related bone metabolism abnormalities under treatment was obtained with the Standardized Uptake Value (SUV)-based information provided by fast whole-body [Tc-99m]-HDP bone tomoscintigraphies (QWBT) recorded with a high-sensitivity CZT-camera / Computed Tomography (CT) hybrid system.
The landscape of fear as a safety eco-field: Experimental evidence
Authors: Farina Almo , James Philip 
Published: November 2022

Abstract
In a development of the ecosemiotic vivo-scape concept, a ‘safety eco-field’ is proposed as a model of a species response to the safety of its environment. The safety eco-field is based on the ecosemiotic approach which considers environmental safety as a resource sought and chosen by individuals to counter predatory pressure. To test the relative safety of different locations within a landscape, 66 bird feeders (BF) were deployed in a regular 15 x 15 m grid in a rural area, surrounded by shrubs, small trees, hedgerows, and buildings. On each of 48 days in November 2021 and February and March 2022, dried mealworms were placed on each BF and counts of larvae at each BF were made at noon and dusk. The European robin (Erithacus rubecula) and the great tit (Parus major) were the most regular visitors to the BFs. Land cover at each BF was recorded. Bird behaviour at the BFs was noted from direct video recordings of the birds at nine selected BFs, totalling 32 daily sessions in March. The different behaviours of the European robin and the great tit were observable. The safety eco-field changed according to the month and the time of day. The distance of the BF from the woodland edges seemed to be important only in the morning. In the afternoon, BFs that were more distant from the woodland edges received the highest number of visits. A significant relationship between land cover and the number of mealworm larvae removed from the BFs was observed. Within the grid of BF, three regions were distinguishable which were related to land cover in the safety eco-field process. The experimental framework confirms the adequacy, at least for birds that have cryptic predators, to map the landscape as a proxy of safety resource. Weather conditions were found to influence the number of mealworms removed, but this requires further investigation. From the video recordings it was noted that the European robin visits were distributed throughout the day without apparent temporal preferences, while the great tit visits were concentrated in the central part of the day. This result has the limitation of the short period of observation (March) and should be extended to the entire period of the experiment to eventually capture seasonal variation. The experimental evidence obtained confirms that the ecosemiotic-based models of safety eco-field are an efficient approach to explain bird feeding preferences and behaviours.