bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adding corn meal into mixed elephant grass–butterfly pea legume silages improves nutritive value and dry matter recovery
Authors: Costa E. R. , Mello A. C. L. , Guim A. , Costa S. B. M. , Abreu B. S. , Silva P. H. F. , Silva V. J. , Simões Neto D. E. 
Published: June 2022

AbstractThe objective of this study was to describe and explain the effect of adding corn meal (CM) on losses, fermentation characteristics and nutritional value of silages from two elephant grass [Cenchrus purpureus (Schumach.) Morrone] genotypes (Taiwan A-146 2.37 and IRI-381) mixed with butterfly pea (Clitoria ternatea L.) legume. The forage was harvested at 75 days of regrowth from elephant grass plots intercropped with butterfly pea legume and ensiled with or without CM at 5% of dry matter (DM) content. Greater gas losses (12 g/kg) and pH (4.2) were observed in the Taiwan A-146 2.37 + butterfly pea silages. The greatest crude protein content was observed in the ‘Taiwan A-146 2.37’ + butterfly pea silage added with CM (116 g/kg). Silages with additive and those containing IRI-381 had a greater acid detergent fibre content (367 and 366 g/kg, respectively). CM increased the silage DM (221 g/kg), remaining water-soluble carbohydrates contents (26 g/kg) and in vitro digestibility of DM. The aerobic stability was maintained until 45 h after opening the silos. All silages presented a good fermentative profile and were not affected by the relatively large proportion of butterfly pea (>34%) in the ensiled mass as indicated by the reduced contents of butyric acid and ammonia nitrogen. CM reduces total losses, increases DM recovery and improves the nutritional value of silages from mixed elephant grass–butterfly pea legume.
Incidence and severity of SARS-CoV-2 infection in former Q fever patients as compared to the Dutch population, 2020–2021
Authors: den Boogert Elisabeth Maria , de Lange Marit M. A. , Wielders Cornelia C. H. , Rietveld Ariene ,   RIVM COVID-19 Surveillance and Epidemiology Team, Knol Mirjam J. , van Gageldonk-Lafeber Arianne B. 
Published: June 2022

Abstract
Surveillance data shows a geographical overlap between the early coronavirus disease 2019 (COVID-19) pandemic and the past Q fever epidemic (2007–2010) in the Netherlands. We investigated the relationship between past Q fever and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in 2020/2021, using a retrospective matched cohort study.
In January 2021, former Q fever patients received a questionnaire on demographics, SARS-CoV-2 test results and related hospital/intensive care unit (ICU) admissions. SARS-CoV-2 incidence with 95% confidence intervals (CI) in former Q fever patients and standardised incidence ratios (SIR) to compare to the age-standardised SARS-CoV-2 incidence in the general regional population were calculated.
Among 890 former Q fever patients (response rate: 68%), 66 had a PCR-confirmed SARS-CoV-2 infection. Of these, nine (14%) were hospitalised and two (3%) were admitted to ICU. From February to June 2020 the SARS-CoV-2 incidence was 1573/100 000 (95% CI 749–2397) in former Q fever patients and 695/100 000 in the general population (SIR 2.26; 95% CI 1.24–3.80). The incidence was not significantly higher from September 2020 to February 2021.
We found no sufficient evidence for a difference in SARS-CoV-2 incidence or an increased severity in former Q fever patients vs. the general population during the period with widespread SARS-CoV-2 testing availability (September 2020–February 2021). This indicates that former Q fever patients do not have a higher risk of SARS-CoV-2 infection.
Effects of EGF and melatonin on gene expression of cumulus cells and furtherin vitroembryo development in bovines
Authors: Cordova A. , Miranda M. S. , King W. A. , Mastromonaco G. F. 
Published: June 2022

SummaryDespite previous research demonstrating the benefits of including growth factors and antioxidants to maturation medium to support embryo production, to date the effect of epidermal growth factor (EGF) and melatonin (Mel) on oocyte competency has not been studied. This study supplementedin vitromaturation (IVM) medium with EGF (10 ng/ml) and Mel (50 ng/ml) alone, or in combination, and evaluated cumulus cell (CC) gene expression and the development and quality of parthenogenetic blastocysts. No differences in CC gene expression levels indicative of developmental potential were found among the treatment groups. Antioxidant geneCuZnSODwas significantly (P< 0.05) decreased in CCs from the Mel group. Moreover, blastocyst rates on day 7 were significantly increased in EGF or Mel (P< 0.05), but not EGF+Mel. Significant decrease (P< 0.05) inGPX1,CuZnSOD,SLC2A1andHSPA1A(P= 0.07) mRNA levels was observed in blastocysts from the Mel group.OCT4gene expression was significantly increased (P< 0.05) in EGF+Mel and confirmed using immunofluorescence. Our results indicate that, despite the lack of changes of competence-related genes in CCs, IVM medium supplemented with Mel improved the culture environment sufficiently, resulting in improved blastocysts. Moreover, EGF and Mel combined during maturation increasedOCT4gene and protein expression in blastocysts, indicating its potential for stem cells.
Improved embryonic development and utilization rates with EmbryoScope: a within-subject comparison versus a benchtop incubator
Authors: Setti Amanda Souza , Braga Daniela Paes de Almeida Ferreira , Vingris Livia , Iaconelli Assumpto , Borges Edson 
Published: June 2022

SummaryThe objective of this study was to investigate whether, in consecutive intracytoplasmic sperm injection (ICSI) cycles, embryonic development in an incubator with a time-lapse imaging (TLI) system is better than the previous one obtained in a benchtop incubator (G-185) with similar cultivation characteristics. The study was of a retrospective within-subject design, in which each cycle served as its own control. Data were obtained via the chart review of patients undergoing ICSI in a private university-affiliated in vitro fertilization (IVF) centre who fulfilled the following criteria: second ICSI attempt in which embryos were cultured in a TLI incubator system (TLI group, n = 71), preceded by a first ICSI attempt in which embryos were cultured in a benchtop incubator (Control group, n = 71). Embryonic development up to the fifth day of development, oocyte utilization rate (OUR; transferred embryos plus frozen embryos per total number of retrieved oocytes) and embryo utilization rate (EUR; transferred embryos plus frozen embryos per normally fertilized oocyte) were compared between the groups. There were significant differences in the day 2 non-cleavage rate, day 5 embryo rate, blastocyst development rate, frozen blastocyst rate, OUR, and EUR, in favour of the TLI group. Embryonic development, frozen blastocyst rate, OUR and EUR in the second ICSI cycle were significantly improved when the culture was performed in the EmbryoScope, compared with those rates obtained with culture in a G-185 in the first ICSI cycle of the same patients. The results may also lead to higher cumulative pregnancy outcomes following embryo thawing and transfer.
Geodynamic Modeling With Uncertain Initial Geometries
Authors: Spang A. , Baumann T. S. , Kaus B. J. P. 
Published: June 2022

AbstractGeodynamic codes have become fast and efficient enough to facilitate sensitivity analysis of rheological parameters. With sufficient data, they can even be inverted for. Yet, the geodynamic inverse problem is often regularized by assuming a constant geometry of the geological setting (e.g., shape, location and size of salt diapirs or magma bodies) or approximating irregular bodies with simple shapes like boxes, spheres or ellipsoids to reduce the parameter space. Here, we present a simple and intuitive method to parameterize complex 3D bodies and incorporate them into geodynamic inverse problems. The approach can automatically create an entire ensemble of initial geometries, enabling us to account for uncertainties in imaging data. Furthermore, it allows us to investigate the sensitivity of the model results to geometrical properties and facilitates inverting for them. We demonstrate the method with two examples. A salt diapir in an extending regime and free subduction of an oceanic plate under a continent. In both cases, small differences in the model's initial geometry lead to vastly different results. Be it the formation of faults or the velocity of plates. Using the salt diapir example, we demonstrate that, given an additional geophysical observation, we are able to invert for uncertain geometric properties. This highlights that geodynamic studies should investigate the sensitivity of their models to the initial geometry and include it in their inversion framework. We make our method available as part of the open‐source software geomIO.
Low‐Angle Normal Faults on the NW Shelf of Australia: Implications for Late Paleozoic Rifting
Authors: Deng Hongdan , McClay Ken , Belgarde Catherine 
Published: June 2022

AbstractLate Paleozoic rifting of the NW Shelf of Australia formed a wide basin that fundamentally controlled the Mesozoic continental rifting and passive margin development; however, structural style associated with this extensional process remains poorly constrained. Here, we integrate high‐resolution seismic data with well data from the proximal domain of the NW Shelf to show that the Mermaid fault system in the eastern Dampier Sub‐basin is a large‐scale (∼80 km long), low‐angle (5–20°) normal fault with up to 10 km offset formed during the Late Paleozoic rifting. In its northern and southern domains, the Mermaid fault has a planar geometry with characteristic extensional structures. In its central domain, seaward‐dipping, domino‐style normal faults in the upper crust extend downward into a highly reflective seismic zone that arches upward to form an elliptical dome. Growth strata in the hangingwall half‐graben and footwall basement reflections indicate that the Mermaid fault initiated as a high‐angle normal fault and was rotated by footwall exhumation during extension. Intrabasement deformation along and below the Mermaid fault is characterized by moderate‐ to high‐amplitude, semi‐continuous seismic reflectors that are distinct from transparent reflection character in the overlying basement unit. Comparison of the Mermaid fault system to well‐studied detachment faults in metamorphic core complex settings reveals striking similarities. We therefore suggest that the Mermaid fault developed as part of a nascent metamorphic core complex with a warm to hot footwall that enabled middle‐lower crustal flow during the Late Paleozoic continental rifting, likely induced by southward subduction of the Paleo‐Tethys Ocean.
Non‐Stationary Influences of Large‐Scale Climate Drivers on Low Flow Extremes in Southeast Australia
Authors: Goswami Pallavi , Peterson Tim J. , Mondal Arpita , Rüdiger Christoph 
Published: June 2022

AbstractThe intensity, duration, and frequency (IDF) of low streamflow events are often considered stationary in time. Given droughts are likely to become more frequent and extreme, here those IDFs are analyzed for their non‐stationarity in relation to three climate drivers, namely, the El Niño‐Southern Oscillation, the Indian Ocean Dipole, and the Southern Annular Mode. For this, low flow events were identified for 161 unregulated catchments in Victoria, Australia. The relationship between the climate drivers and the IDFs were developed using covariate‐based non‐stationary statistical models. We found that several of these catchments exhibit strong non‐stationary influences from the climate modes. Such catchments have a higher probability of experiencing more intense, prolonged, and/or frequent low flows when a dry phase of these climate drivers occurs. We found that catchments having strong evidence of non‐stationary frequency (n = 77) were more compared to those having non‐stationary intensity (n = 19) or duration (n = 34). Further, we derived projected indices of these climate drivers from CMIP5 models for the RCP8.5 scenario. These indicate an intensification in the dry phase of the climate drivers. Using information of the projected indices in the IDF models, we found that future dry phases of these climate drivers are likely to further increase the probability of experiencing higher magnitudes of low flow IDFs in catchments non‐stationary in these characteristics in relation to one or more of these climate drivers. Frequency shows greater response to future dry phases of these drivers than intensity or duration of low flows.
Burial and Exhumation of Sedimentary Rocks Revealed by the Base Stimson Erosional Unconformity, Gale Crater, Mars
Authors: Watkins Jessica A. , Grotzinger John P. , Stein Nathan T. , Banham Steven G. , Gupta Sanjeev , Rubin David M. , Morgan Kathryn Stack , Edgett Kenneth S. , Frydenvang Jens , Siebach Kirsten L. , Lamb Michael P. , Sumner Dawn Y. , Lewis Kevin W. 
Published: June 2022

AbstractSedimentary rocks record the ancient climate of Mars through changes between subaqueous and eolian depositional environments, recognized by their stratal geometries and suites of sedimentary structures. Orbiter‐ and rover‐image‐based geologic mapping show a dynamic evolution of the 5‐km‐thick sedimentary sequence exposed along the flanks of Aeolis Mons (informally, Mt. Sharp) in Gale crater, Mars, by deposition of subaqueous strata followed by exhumation via eolian erosion and then deposition of overlying, onlapping strata of inferred eolian origin. This interpretation suggests that a significant unconformity should occur at the base of the onlapping strata, thus predicting lateral variations in elevation along the contact between the underlying Mt. Sharp group and overlying Stimson formation. Curiosity rover and high‐resolution orbital image data quantify paleotopographic variability associated with the contact; ∼140 m of net elevation change and a slope closely aligned with the modern topography is expressed along the regional contact. These results support the interpretation of an erosional unconformity between these strata and that it was likely formed as a result of eolian erosion within the crater, indicative of a transition from wet to dry climate and providing insight into the stratigraphic context, geologic history, and habitability within Gale crater.
Extracellular sialyltransferase st6gal1 in breast tumor cell growth and invasiveness
Authors: Hait Nitai C. , Maiti Aparna , Wu Rongrong , Andersen Valerie L. , Hsu Chang-Chieh , Wu Yun , Chapla Digantkumar G. , Takabe Kazuaki , Rusiniak Michael E. , Bshara Wiam , Zhang Jianmin , Moremen Kelley W. , Lau Joseph T. Y. 
Published: June 2022

AbstractThe sialyltransferase ST6GAL1 that adds α2–6 linked sialic acids to N-glycans of cell surface and secreted glycoproteins is prominently associated with many human cancers. Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion and resistance to cell stress and chemo- and radio-treatments. Canonically, ST6GAL1 resides in the intracellular secretory apparatus and glycosylates nascent glycoproteins in biosynthetic transit. However, ST6GAL1 is also released into the extracellular milieu and extracellularly remodels cell surface and secreted glycans. The impact of this non-canonical extrinsic mechanism of ST6GAL1 on tumor cell pathobiology is not known. We hypothesize that ST6GAL1 action is the combined effect of natively expressed sialyltransferase acting cell-autonomously within the ER-Golgi complex and sialyltransferase from extracellular origins acting extrinsically to remodel cell-surface glycans. We found that shRNA knockdown of intrinsic ST6GAL1 expression resulted in decreased ST6GAL1 cargo in the exosome-like vesicles as well as decreased breast tumor cell growth and invasive behavior in 3D in vitro cultures. Extracellular ST6GAL1, present in cancer exosomes or the freely soluble recombinant sialyltransferase, compensates for insufficient intrinsic ST6GAL1 by boosting cancer cell proliferation and increasing invasiveness. Moreover, we present evidence supporting the existence novel but yet uncharacterized cofactors in the exosome-like particles that potently amplify extrinsic ST6GAL1 action, highlighting a previously unknown mechanism linking this enzyme and cancer pathobiology. Our data indicate that extracellular ST6GAL1 from remote sources can compensate for cellular ST6GAL1-mediated aggressive tumor cell proliferation and invasive behavior and has great clinical potential for extracellular ST6GAL1 as these molecules are in the extracellular space should be easily accessible targets.
IGF2BP3 enhances the mRNA stability of E2F3 by interacting with LINC00958 to promote endometrial carcinoma progression
Authors: Wang Cuicui , Kong Fanfei , Ma Jian , Miao Jianing , Su Peng , Yang Hui , Li Qing , Ma Xiaoxin 
Published: June 2022

AbstractLong noncoding RNAs (lncRNAs) play important regulatory roles in a variety of pathological processes involving cancer. However, the exact molecular mechanisms of lncRNA regulation in endometrial carcinoma (EC) remain poorly defined. The aim of this study was to illustrate the mechanism of LINC00958 in regulating the function of IGF2BP3, an RNA binding protein involved in mRNA stability, and their clinical implications in EC. First, we investigated the clinical role of IGF2BP3 in EC and demonstrated its prognostic value. Loss-of-function and gain-of-function studies showed that IGF2BP3 promoted EC cell proliferation, migration and invasion. Then, we carried out RNA immunoprecipitation sequencing (RIP-seq) analysis, RNA pulldown and immunofluorescence-RNA fluorescence in situ hybridization to identify LINC00958 that interacted with IGF2BP3 in the cytoplasm of EC cells. Rescue experiments indicated that knockdown of LINC00958 partially offset the EC cell progression mediated by IGF2BP3. After that, RNA sequencing was used to screen out the downstream genes of IGF2BP3 and LINC00958. The results revealed that IGF2BP3 upregulated E2F3 expression by interacting with LINC00958. Furthermore, RNA stability assays demonstrated that silencing LINC00958 partially rescued the IGF2BP3-mediated promoting effect on the mRNA stability of E2F3. Collectively, this study suggests that LINC00958, as an oncogene, assists IGF2BP3 in stabilizing E2F3 mRNA and ultimately promotes EC progression, providing a promising therapeutic target for patients with EC.
Disturbance-based management of ecosystem services and disservices in partial nitritation-anammox biofilms
Authors: Suarez Carolina , Sedlacek Christopher J. , Gustavsson David J. I. , Eiler Alexander , Modin Oskar , Hermansson Malte , Persson Frank 
Published: June 2022

AbstractThe resistance and resilience provided by functional redundancy, a common feature of microbial communities, is not always advantageous. An example is nitrite oxidation in partial nitritation-anammox (PNA) reactors designed for nitrogen removal in wastewater treatment, where suppression of nitrite oxidizers likeNitrospirais sought. In these ecosystems, biofilms provide microhabitats with oxygen gradients, allowing the coexistence of aerobic and anaerobic bacteria. We designed a disturbance experiment where PNA biofilms, treating water from a high-rate activated sludge process, were constantly or intermittently exposed to anaerobic sidestream wastewater, which has been proposed to inhibit nitrite oxidizers. With increasing sidestream exposure we observed decreased abundance, alpha-diversity, functional versatility, and hence functional redundancy, amongNitrospirain the PNA biofilms, while the opposite patterns were observed for anammox bacteria withinBrocadia. At the same time, species turnover was observed for aerobic ammonia-oxidizingNitrosomonaspopulations. The different exposure regimens were associated with metagenomic assembled genomes ofNitrosomonas,Nitrospira, andBrocadia, encoding genes related to N-cycling, substrate usage, and osmotic stress response, possibly explaining the three different patterns by niche differentiation. These findings imply that disturbances can be used to manage the functional redundancy of biofilm microbiomes in a desirable direction, which should be considered when designing operational strategies for wastewater treatment.
Ultrastretchable and wearable conductive multifilament enabled by buckled polypyrrole structure in parallel
Authors: Li Yimeng , Gao Yaya , Lan Lizhen , Zhang Qian , Wei Leqian , Shan Mengqi , Guo Lamei , Wang Fujun , Mao Jifu , Zhang Ze , Wang Lu 
Published: June 2022

AbstractStretchable conductive fibers have attracted much attention due to their potential use in wearable electronics. However, the ultra-high strain insensitive conductivity is hindered by mechanical mismatch in Young’s modulus and failure of stretchable structures under large deformation. This challenge is addressed with a conductive and elastic multifilament made of the polyurethane monofilaments that are surface-coated with buckled polypyrrole (PPy) of which flexibility is improved by sodium sulfosalicylate. Such parallel conductive monofilaments with PPy buckling on surface reduce the influence of cracks in the conductive coating on the overall conductivity, displaying an ultra-high strain insensitive behavior (quality factor Q = 10.9). Remarkably, various complex forms of wearable electronic textiles made by this conductive multifilament maintain the strain-insensitive behavior of the original multifilament, even upon the large deformation of human joint. This multifilament with wrinkled PPy has attractive advantages in the application of super-stretched wearable electronic devices.
Quantum dynamics of topological strings in a frustrated Ising antiferromagnet
Authors: Zhou Zheng , Liu Changle , Yan Zheng , Chen Yan , Zhang Xue-Feng 
Published: June 2022

AbstractWe investigate the quantum dynamics of the antiferromagnetic transverse field Ising model on the triangular lattice through large-scale quantum Monte Carlo simulations and stochastic analytic continuation. This model effectively describes a series of triangular rare-earth compounds, for example, TmMgGaO4. At weak transverse field, we capture the excitations related to topological quantum strings, which exhibit continuum features described by XY chain along the strings and those in accord with ‘Luttinger string liquid’ in the perpendicular direction. The continuum features can be well understood from the perspective of topological strings. Furthermore, we identify the contribution of strings from the excitation spectrum. Our study provides characteristic features for the experimental search for string-related excitations and proposes a theoretical method to pinpoint topological excitations in the experimental spectra.