bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patient-reported outcome measures for pain in women with pelvic floor disorders: a systematic review
Authors: Ralphsmith Maisie , Ahern Susannah , Dean Joanne , Ruseckaite Rasa 
Published: March 2022

Abstract
Introduction and hypothesis
Patient-reported outcome measures (PROMs) are helpful instruments when measuring and reporting changes in patient health status (Al Sayah et al. J Patient Rep Outcomes 5 (Suppl 2):99, 2021) such as the health-related quality of life (HrQoL) of women with pelvic organ prolapse (POP) and stress urinary incontinence (SUI). The Australasian Pelvic Floor Procedure Registry (APFPR) aims to increase capacity for women to report surgical outcomes through the collection of HrQoL data (Ruseckaite et al. Qual Life Res. 2021) but currently lacks a pain-specific PROM for women with pelvic floor disorders (PFDs), particularly POP and SUI. This review aims to systematically review the existing literature and identify instruments that measure pain in women with POP and SUI for inclusion within the APFPR, which reports on complications from these conditions.

Methods
We conducted a literature search on OVID MEDLINE, Embase, CINAHL, PsycINFO and EMCARE databases in addition to Google Scholar and grey literature to identify studies from inception to April 2021. Full-text studies were included if they used PROMs to measure pain in women with POP and SUI. Two authors independently screened articles, extracted data and assessed methodological quality.

Results
From 2001 studies, 23 publications describing 19 different PROMs were included for analysis. Eight of these instruments were specific to the pelvic floor; four were only specific to pain and used across multiple disorders; three were generic quality of life instruments and four were other non-validated instruments such as focus group interviews. These instruments were not specific to pain in women with POP or SUI, as they did not identify all relevant domains such as the sensation, region and duration of pain, or incidents where onset of pain occurs.

Conclusions
The findings of this review suggest there are no current PROMs that are suitable pain-specific instruments for women with POP or SUI. This knowledge may inform and assist in the development of a new PROM to be implemented into the APFPR.
A subcellular cookie cutter for spatial genomics in human tissue
Authors: Bury Alexander G. , Pyle Angela , Marcuccio Fabio , Turnbull Doug M. , Vincent Amy E. , Hudson Gavin , Actis Paolo 
Published: March 2022

AbstractIntracellular heterogeneity contributes significantly to cellular physiology and, in a number of debilitating diseases, cellular pathophysiology. This is greatly influenced by distinct organelle populations and to understand the aetiology of disease, it is important to have tools able to isolate and differentially analyse organelles from precise location within tissues. Here, we report the development of a subcellular biopsy technology that facilitates the isolation of organelles, such as mitochondria, from human tissue. We compared the subcellular biopsy technology to laser capture microdissection (LCM) that is the state-of-the-art technique for the isolation of cells from their surrounding tissues. We demonstrate an operational limit of  >20 µm for LCM and then, for the first time in human tissue, show that subcellular biopsy can be used to isolate mitochondria beyond this limit.Graphical abstract
Ultra-high-performance liquid chromatography-atmospheric pressure ionization-tandem mass spectrometry method for the migration studies of primary aromatic amines from food contact materials
Authors: Arrizabalaga-Larrañaga Ane , de Juan-de Juan Pedro , Bressan Claudia , Vázquez-Espinosa Mercedes , González-de-Peredo Ana V. , Santos F. Javier , Moyano Encarnación 
Published: March 2022

AbstractThis work describes the development of an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the determination of 23 primary aromatic amines (PAAs) that can potentially migrate from food contact materials. The chromatographic separation was performed in a pentafluorophenylpropyl (PFPP) column achieving the separation of all PAAs in less than 6.5 min using water to acetonitrile (0.1% acetic acid in both solvents) as mobile phase and a gradient elution. The feasibility of atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) was evaluated as alternative to electrospray ionization (ESI) for the analysis of PAAs. Results showed that for most of the compounds, better responses were obtained with APCI, which shows the advantage of being less susceptible to matrix effects. Tandem mass spectrometry (MS/MS) fragmentation studies of [M + H]+ allowed for the selection of the two most characteristic and abundant product ions of the 23 PAAs which led to the development of a selective and sensitive UHPLC-APCI-MS/MS method with limits of detection ranging from 0.2 to 2 μg kg−1. Moreover, intra-day and inter-day precisions of the method in terms of relative standard deviation (RSD%) were lower than 10% and 15%, while trueness as relative error was <15% for most of the compounds. The UHPLC-APCI-MS/MS method was applied to the analysis of twenty black Nylon kitchenware samples that were submitted to migration tests using food simulant B (3% acetic acid, w/v), and the presence of PAAs were detected in eighteen samples at concentrations above the legislated limit (2 μg kg−1 of food or food simulants).
Graphical abstract
Three-dimensional static-fluid MR urography with gradient- and spin-echo (GRASE) at 3.0T: comparison of image quality and diagnostic performance with respiratory-triggered fast spin-echo (FSE)
Authors: Wang Wei , Yang Junzhe , Liu Jing , Li Wei , Zhao Kai , Xue Ke , Dai Yongming , Qiu Jianxing 
Published: March 2022

Abstract
Purpose
To compare the performance of 3D MRU based on a breath-hold gradient- and spin-echo (BH-GRASE) technique with conventional 3D respiratory-triggered FSE (RT-FSE) sequence in patients with urinary tract dilation.

Methods
We prospectively included 90 patients with urinary tract dilation who underwent both 3D BH-GRASE and RT-FSE MRU at 3T. The acquisition time of two MRU sequences was recorded. Three readers blinded to the protocols reviewed the image quality using a five-point scale and assessed the diagnostic performance related to urinary tract dilation. The relative contrast ratio (CR) between the urinary tract and adjacent area was measured quantitatively.

Results
Acquisition time was 14.8 s for BH-GRASE MRU and 213.6 ± 52.2 s for RT-FSE MRU. The qualitative image analysis demonstrated significant equivalence between the two MRU protocols. 3D BH-GRASE MRU better depicted bilateral renal calyces than RT-FSE MRU (p < 0.05). The CR values of the urinary tract were lower on BH-GRASE MRU compared with RT-FSE MRU (p < 0.05). There were excellent agreements in the assessment of urinary tract dilation between BH-GRASE and RT-FSE MRU, including the dilated degree, obstructive level, and obstructive imaging features (inter-sequence κ = 0.924–1).

Conclusion
3D BH-GRASE MRU significantly decreased the acquisition time and achieved comparable image quality, urinary tract visualization, and diagnostic performance with conventional 3D RT-FSE MRU. Breath-hold 3D MRU with GRASE may provide a feasible evaluation of urinary tract dilation.

Graphical abstract
Agricultural resources in the Bronze Age city of Tel Lachish
Authors: Nicolì Marco , Riehl Simone , Webster Lyndelle , Streit Katharina , Höflmayer Felix 
Published: March 2022

AbstractIn this paper, we present the results of the plant macrofossil analyses from the site of Tel Lachish, Israel with focus on the botanical assemblage of the Middle and Late Bronze Age layers collected in two different areas of the tell: Area S, a trench on the western edge of the site, whose samples belong to Late Bronze Age deposits, and Area P, the palace area on the top of the mound with samples ranging from the Middle to Late Bronze Age. Systematic sampling of these areas and analysis of the remains have extended our knowledge of the agricultural resources of one of the most influential Late Bronze Age cities in the southern Levant. Multivariate statistics have been applied to gain insight into regional patterns of crop growing. Fruit crops account for the majority of the identified remains from this site, which also included large quantities of Hordeum vulgare (barley) and Triticum dicoccum (emmer wheat) grains. The virtual lack of chaff remains is not solely a matter of preservation, since the Late Bronze Age assemblage preserved fragile small seeds. Rather, this finding suggests that cereal processing took place some distance from the area of deposition. Overall high diversity, ubiquity and proportions of fruit crops indicate that these played a fundamental role in their cultivation and probably also in cultural life at Lachish throughout the 15th–12th centuries bce.
Acute, isolated fractures of the metatarsal bones: an epidemiologic study
Authors: Herterich Viktoria , Hofmann Luzie , Böcker Wolfgang , Polzer Hans , Baumbach Sebastian Felix 
Published: March 2022

AbstractIntroductionAlthough metatarsal fractures are common, the significance of previous epidemiologic studies is limited to specific fracture entities, subpopulations, or heterogeneous fracture aetiologies. The aim of the study was to assess the epidemiology of isolated metatarsal fractures in an adult population at a level-1 trauma centre.Materials and methodsRadiological and clinical databases were searched for a five-year period. Eligible were all patients with acute isolated metatarsal fractures over the age of 18 years with radiographs in two planes available. Stress fractures, injuries affecting Lisfranc joint stability, and concomitant injuries to other regions than the metatarsals were excluded. Data collection included general demographics, mechanism of injury, season of the trauma and fracture details.ResultsOut of 3259 patients, 642 patients met the inclusion criteria and were included for the analysis. The patients’ mean age was 44.5 ± 18.9 years, 50.6% were female. 83.3% suffered an isolated, 16.7% multiple metatarsal fractures. Single metatarsal fractures occurred predominantly at the fifth metatarsal bone (81.3%), their frequency decreased with increasing age, with a seasonal peak during the summer. Patients suffering multiple metatarsal fractures were significantly older (51.6 ± 21.2 vs. 43.0 ± 18.1 years;p < 0.001) and the injury resulted significantly more often from a high-energy trauma (6.7% vs. 23.4%;p < 0.001). Multiple metatarsal fractures occurred evenly throughout all metatarsals but revealed a focus on female population with no seasonal differences.ConclusionSingle metatarsal fractures predominantly occurred at the fifth metatarsal bone and showed a seasonal, gender and age dependency. Multiple metatarsal fractures were homogeneously distributed between the different metatarsals with distinct age-dependent gender differences.Level of evidenceLevel III.
A disaster victim identification workshop focused on forensic odontology using embalmed human remains
Authors: Zwirner Johann , Duncan Warwick 
Published: March 2022

AbstractA high number of victims of mass casualty incidences are identified through their teeth. While forensic odontologists need to have a complex skillset during a disaster victim identification (DVI) response, hands-on training opportunities are rare. In countries with very limited forensic casework, such as New Zealand, many forensic odontologists find it difficult to achieve the number of annual forensic dental identifications required to maintain their credentialling. This report details the development of a hands-on forensic odontology-focused DVI workshop using human Crosado-embalmed remains. Anonymous participant evaluations, including five-point Likert and open-ended items, were performed in both years the workshop was held. A total of 10 and 17 participants, predominantly dentists, attended the workshop in 2020 and 2021, respectively. Participant feedback was extremely positive. Likert items were statistically similar between participants in both years. Open-ended items revealed positive feedback regarding the use of cadaveric remains, the gained hands-on experience, or the teamwork aspect. Participants who attended the workshop in both years commented on the positive aspect of repetition to cement their skills. As areas of improvement, participants named (for example) time management and the number of portable X-ray devices, leading to changes that were implemented in 2021. Moreover, the participants expressed interest to further their skills on decomposed, burnt, and fragmented human remains, which for ethical reasons has yet to be implemented. The DVI workshop described here, using embalmed human remains, provides an opportunity to add dental identifications toward annual credentialling requirements for forensic odontologists. Participants rated the course to be excellent overall and highly relevant for their role. For future workshops, there is an interest to include further aspects of the DVI response such as fingerprinting or police work as well as remains, which are altered due to natural or physical reasons.
Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies
Authors: Brenna Cinzia , Simioni Carolina , Varano Gabriele , Conti Ilaria , Costanzi Eva , Melloni Mattia , Neri Luca Maria 
Published: March 2022

AbstractUnderstanding the inner morphology of intact tissues is one of the most competitive challenges in modern biology. Since the beginning of the twentieth century, optical tissue clearing (OTC) has provided solutions for volumetric imaging, allowing the microscopic visualization of thick sections of tissue, organoids, up to whole organs and organisms (for example, mouse or rat). Recently, tissue clearing has also been introduced in clinical settings to achieve a more accurate diagnosis with the support of 3D imaging. This review aims to give an overview of the most recent developments in OTC and 3D imaging and to illustrate their role in the field of medical diagnosis, with a specific focus on clinical applications.Graphical abstract
Effects of vision on energy expenditure and kinematics during level walking
Authors: Eiken Ola , Mekjavic Igor B. , Babič Jan , Danielsson Ulf , Hallberg Magnus , Kounalakis Stylianos N. 
Published: March 2022

Abstract
Purpose
We have previously observed substantially higher oxygen uptake in soldiers walking on terrain at night than when performing the same walk in bright daylight. The aims of the present study were to investigate the influence of vision on mechanical efficiency during slow, horizontal, constant-speed walking, and to determine whether any vision influence is modified by load carriage.

Methods
Each subject (n = 15) walked (3.3 km/h) for 10 min on a treadmill in four different conditions: (1) full vision, no carried load, (2) no vision, no carried load, (3) full vision with a 25.5-kg rucksack, (4) no vision with a 25.5-kg rucksack.

Results
Oxygen uptake was 0.94 ± 0.12 l/min in condition (1), 1.15 ± 0.20 l/min in (2), 1.15 ± 0.12 l/min in (3) and 1.35 ± 0.19 l/min in (4). Thus, lack of vision increased oxygen uptake by about 19%. Analyses of movement pattern, by use of optical markers attached to the limbs and torso, revealed considerably shorter step length (12 and 10%) in the no vision (2 and 4) than full vision conditions (1 and 3). No vision conditions (2 and 4) increased step width by 6 and 6%, and increased vertical foot clearance by 20 and 16% compared to full vision conditions (1 and 3).

Conclusion
The results suggest that vision has a marked influence on mechanical efficiency even during entrained, repetitive movements performed on an obstacle-free horizontal surface under highly predictable conditions.
Hydroxycinnamoyltransferase and CYP98 in phenolic metabolism in the rosmarinic acid-producing hornwort Anthoceros agrestis
Authors: Ernst Lucien , Wohl Julia , Bauerbach Elke , Petersen Maike 
Published: March 2022

Abstract
Main conclusion
Anthoceros agrestis hydroxycinnamoyltransferase accepts shikimic and 3-hydroxyanthranilic acids while hydroxycinnamoylester/amide 3-hydroxylase (CYP98A147) preferred p-coumaroyl-(3-hydroxy)anthranilic acid compared to the shikimic acid derivative. Alternative pathways towards rosmarinic acid have to be considered.

Abstract
Rosmarinic acid (RA) is a well-known ester of caffeic acid and 3,4-dihydroxyphenyllactic acid. In the search for enzymes involved in RA biosynthesis in the hornwort Anthoceros agrestis, the hydroxycinnamoyltransferase sequence with the highest similarity to rosmarinic acid synthase from Lamiaceae has been amplified and heterologously expressed in Escherichia coli. In parallel, the single cytochrome P450 sequence belonging to the CYP98 group in Anthoceros agrestis was isolated and expressed in Saccharomyces cerevisiae which did not result in protein formation. Codon optimization and co-expression with NADPH:cytochrome P450 reductase (CPR) from Coleus blumei resulted in the formation of active enzymes. Both, the hydroxycinnamoyltransferase and CYP98 were characterized with respect to their temperature and pH optimum as well as their substrate acceptance. The hydroxycinnamoyltransferase (AaHCT6) readily accepted p-coumaroyl- and caffeoyl-CoA with a slightly higher affinity towards p-coumaroyl-CoA. The best acceptor substrate was shikimic acid (Km 25 µM with p-coumaroyl-CoA) followed by 3-hydroxyanthranilic acid (Km 153 µM with p-coumaroyl-CoA). Another accepted substrate was 2,3-dihydroxybenzoic acid. Anthranilic acid and 4-hydroxyphenyllactic acid (as precursor for RA) were not used as substrates. p-Coumaroylesters and -amides are substrates hydroxylated by CYP98 hydroxylases. The only CYP98 sequence from Anthoceros agrestis is CYP98A147. The best substrates for the NADPH-dependent hydroxylation were p-coumaroylanthranilic and p-coumaroyl-3-hydroxyanthranilic acids while p-coumaroylshikimic and p-coumaroyl-4-hydroxyphenyllactic acids were poor substrates. The biosynthetic pathway towards rosmarinic acid thus still remains open and other enzyme classes as well as an earlier introduction of the 3-hydroxyl group to afford the caffeic acid substitution pattern must be taken into consideration.
Burden of hospitalizations and outpatient visits associated with moderate and severe acute graft-versus-host disease in Finland and Sweden: a real-world data study
Authors: Sabatelli Lorenzo , Keränen Mikko , Viayna Elisabet , Roset Montserrat , Lara Nuria , Thunström Daniel , Pfeiffer Minja , Nicklasson Malin , Itälä-Remes Maija 
Published: March 2022

Abstract

Purpose
The aim of this study was to describe patient characteristics and quantify hospital stays and outpatient visits (H&OV) following diagnosis with moderate-to-severe acute graft-versus-host disease (aGVHD) in Finland and Sweden.

Methods
A retrospective chart audit collected data from patient medical records of 3 specialized centers performing allogeneic hematopoietic stem cell transplantation (HSCT; Finland, n = 2; Sweden, n = 1). Eligible patients received allogeneic HSCT (January 1, 2016–June 30, 2017) from any donor source, were diagnosed with grade II–IV aGVHD (MAGIC or modified Glucksberg criteria) at any time from transplantation to 12 months before data collection, and were ≥ 18 years old at diagnosis. Criteria for comparing patients graded with modified Glucksberg and MAGIC severity scales were defined.

Results
Fifty-five patients (Finland, n = 45; Sweden, n = 10) were included. Myeloablative conditioning was the most common conditioning regimen (81.8%); immunosuppression regimens were based on combinations of methotrexate (96.4%), in vivo T-cell depletion (80.0%), cyclosporine (63.6%), mycophenolate (40.0%), and tacrolimus (34.5%). Sixteen patients (29.1%) developed grade III/IV aGVHD; skin was the most common organ involved (80.0%). Most patients required ≥ 1 hospital stay (89.1%; median of 2 hospitalizations per patient); 7 patients (14.3%) required admission to an intensive care unit. Median hospitalization duration from HSCT to discharge was 26 days. Most patients also required outpatient or emergency department visits (90.9%). Subgroup analyses showed longer hospital stays for patients receiving multiple treatment lines; no clear differences in H&OV were observed between prophylactic regimens.

Conclusion
Based on this retrospective study, moderate-to-severe aGVHD is associated with considerable healthcare resource utilization in Finland and Sweden, particularly in patients who received multiple lines of therapy.
A novel deep ordinal classification approach for aesthetic quality control classification
Authors: Rosati Riccardo , Romeo Luca , Vargas Víctor Manuel , Gutiérrez Pedro Antonio , Hervás-Martínez César , Frontoni Emanuele 
Published: March 2022

AbstractNowadays, decision support systems (DSSs) are widely used in several application domains, from industrial to healthcare and medicine fields. Concerning the industrial scenario, we propose a DSS oriented to the aesthetic quality control (AQC) task, which has quickly established itself as one of the most crucial challenges of Industry 4.0. Taking into account the increasing amount of data in this domain, the application of machine learning (ML) and deep learning (DL) techniques offers great opportunities to automatize the overall AQC process. State-of-the-art is mainly oriented to approach this problem with a nominal DL classification method which does not exploit the ordinal structure of the AQC task, thus not penalizing the error among distant AQC classes (which is a relevant aspect for the real use case). The paper introduces a DL ordinal methodology for the AQC classification. Differently from other deep ordinal methods, we combined the standard categorical cross-entropy with the cumulative link model and we imposed the ordinal constraint via the thresholds and slope parameters. Experimental results were performed for solving an AQC task on a novel image dataset originated from a specific company’s demand (i.e., aesthetic assessment of wooden stocks). We demonstrated how the proposed methodology is able to reduce misclassification errors (up to 0.937 quadratic weight kappa loss) among distant classes while overcoming other state-of-the-art deep ordinal models and reducing the bias factor related to the item geometry. The proposed DL approach was integrated as the main core of a DSS supported by Internet of Things (IoT) architecture that can support the human operator by reducing up to 90% the time needed for the qualitative analysis carried out manually in this specific domain.