bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influences of Thermal Stress During Three Weeks Before Market Age on Histology and Expression of Genes Associated With Adipose Infiltration and Inflammation in Commercial Broilers, Native Chickens, and Crossbreeds
Authors: Malila Yuwares , Sanpinit Pornnicha , Thongda Wilawan , Jandamook Anuwat , Srimarut Yanee , Phasuk Yupin , Kunhareang Sajee 
Published: April 2022

The objectives of this study were to examine the effects of cyclic thermal stress on histological characteristics of breast muscle and gene expression regarding adipose infiltration and inflammation in breast muscles collected from different breeds of chickens. The birds, from commercial broilers (CB, Ross 308, 3 weeks), native (NT, 100% Thai native Chee, 9 weeks), H75 (crossbred; 75% broiler and 25% NT, 5 weeks), and H50 (crossbred; 50% broiler and 50% NT, 7 weeks), were equally assigned into control or treatment groups. The control samples were reared under a constant temperature of 26 ± 1°C, while the treatment groups were exposed to 35 ± 1°C (6 h per day). After a 20-day thermal challenge, 12 male birds per treatment group were randomly collected for determination of live body weight, breast weight, numbers of growth-related myopathies, and breast meat chemical composition. Histological lesions were evaluated in the pectoralis major muscle immediately collected within 20 min postmortem based on hematoxylin and eosin staining. The results indicated that despite interaction between thermal stress and breed effects, thermal challenge significantly reduced feed intake, live body weight, and breast weight of the birds and increased moisture content in breast meat (p &lt; 0.05). An interaction between the two main factors was found for protein content (p &lt; 0.05) for which control CB showed less protein than the other groups. Heat stress decreased histological scores for adipose infiltration in CB (p &lt; 0.05), but it did not significantly influence such scores in the other groups. CB received histological scores for adipose tissue at greater extent than those for the other groups. Differential absolute abundance of CD36, FABP4, LITAF, PDGFRA, PLIN1, PPARG, POSTN, SCD1, and TGFB1 in the muscle samples well-agreed with the trend of histological scores, suggesting potential involvement of dysregulated fibro-adipogenic progenitors together with imbalanced lipid storage and utilization in the breast muscle. The findings demonstrated that the cyclic thermal challenge restricted growth performance and breast mass of the birds, but such effects attenuated infiltration of adipose tissue and inflammatory cells in the CB breast muscle.
Non-Invasive Quantification of Fraction Flow Reserve Based on Steady-State Geometric Multiscale Models
Authors: Liu Jincheng , Wang Xue , Li Bao , Huang Suqin , Sun Hao , Zhang Liyuan , Sun Yutong , Liu Zhuo , Liu Jian , Wang Lihua , Zhao Xi , Wang Wenxin , Zhang Mingzi , Liu Youjun 
Published: April 2022

Background: The underuse of invasive fraction flow reserve (FFR) in clinical practice has motivated research towards its non-invasive prediction. The early attempts relied on solving the incompressible three-dimensional Navier–Stokes equations in segmented coronary arteries. However, transient boundary condition has a high resource intensity in terms of computational time. Herein, a method for calculating FFR based on steady-state geometric multiscale (FFRSS) is proposed.Methods: A total of 154 moderately stenotic vessels (40–80% diameter stenosis) from 136 patients with stable angina were included in this study to validate the clinical diagnostic performance of FFRSS. The method was based on the coronary artery model segmented from the patient’s coronary CTA image. The average pressure was used as the boundary condition for the inlet, and the microcirculation resistance calculated by the coronary flow was used as the boundary condition for the outlet to calculate the patient-specific coronary hyperemia. Then, the flow velocity and pressure distribution and the FFRss of each coronary artery branch were calculated to evaluate the degree of myocardial ischemia caused by coronary stenosis. Also, the FFRSS and FFRCT of all patients were calculated, and the clinically measured FFR was used as the “gold standard” to verify the diagnostic performance of FFRSS and to compare the correlation between FFRSS and FFRCT.Results: According to the FFRSS calculation results of all patients, FFRSS and FFR have a good correlation (r = 0.68, p &lt; 0.001). Similarly, the correlation of FFRSS and FFRCT demonstrated an r of 0.75 (95%CI: 0.67–0.72) (p &lt; 0.001). On receiver-operating characteristic analysis, the optimal FFRSS cut point for FFR≤0.80 was 0.80 (AUC:0.85 [95% confidence interval: 0.79 to 0.90]; overall accuracy:88.3%). The overall sensitivity, specificity, PPV, and NPV for FFRSS ≤0.80 versus FFR ≤0.80 was 68.18% (95% CI: 52.4–81.4), 93.64% (95% CI: 87.3–97.4), 82.9%, and 91.1%, respectively.Conclusion: FFRSS is a reliable diagnostic index for myocardial ischemia. This method was similar to the closed-loop geometric multiscale calculation of FFR accuracy but improved the calculation efficiency. It also improved the clinical applicability of the non-invasive computational FFR model, helped the clinicians diagnose myocardial ischemia, and guided percutaneous coronary intervention.
Aberrant Salience and Disorganized Symptoms as Mediators of Psychosis
Authors: Ceballos-Munuera Celia , Senín-Calderón Cristina , Fernández-León Sandra , Fuentes-Márquez Sandra , Rodríguez-Testal Juan Fco. 
Published: April 2022

IntroductionIdeas of reference (IR) are frequent in psychopathology, mainly in psychotic disorders. The frequency of IR and preoccupation about them are related to the psychotic dimension, and to a lesser extent, to negative or emotional disorganized dimensions. Aberrant salience (AS), has been proposed as an indicator of the onset of psychosis, particularly of schizophrenia. This study analyzed the mediating role of AS, disorganized symptoms and preoccupation about IR in the relationship between IR and the psychotic dimension.MethodThe sample consisted of 330 participants (116 university students and 214 clinically active patients), 62.4% of whom were women aged 18–79. The Referential Thinking Scale, the Aberrant Salience Inventory, and the Brief Psychiatric Rating Scale were administered.ResultsEvidence of a partial mediation model showed that the relationships between IR and the psychotic dimension were mediated jointly by AS and the disorganized dimension, and preoccupation about IR no longer had a role. This relationship was significantly influenced by participant age. The variables in the model explained 54.16% of the variance.ConclusionThe model proposed enabled a set of vulnerabilities (unusual thought content) to be predicted that could lead to a high-risk general pathological state and proneness to psychosis in particular. These findings are discussed with regard to early detection and prevention of psychosis.
Childhood Maltreatment, Stressful Life Events, Cognitive Emotion Regulation Strategies, and Non-suicidal Self-Injury in Adolescents and Young Adults With First-Episode Depressive Disorder: Direct and Indirect Pathways
Authors: Qian Hong , Shu Chang , Feng Li , Xiang Junyi , Guo Ying , Wang Gaohua 
Published: April 2022

IntroductionChildhood maltreatment (CM), stressful life events (SLE), and cognitive emotion regulation strategies (CERS) have been considered crucial in the development of non-suicidal self-injury (NSSI) and major depressive disorder (MDD), but the pathways of this association are not clear. We aim to identify direct effects of CM and SLE on NSSI and depression severity and its indirect effects via CERS in adolescents and young adults with a diagnosis of MDD.MethodsA total of 114 patients (aged 14–24 years) with first episode MDD were included and further divided into the NSSI group (n = 56) and non-NSSI group (n = 58) according to the DSM-5 criteria. Diagnostic interviews and self-report measures were conducted to assess CM, SLE, CERS, and diagnose NSSI. Severity of depressive symptoms was measured using the Hamilton Rating Scale (HAMD). The structural equation model was used to assess the pathways.ResultsMDD patients with NSSI had more frequent family history of mental illness, more experience of CM and SLE, more serious depression, less use of adaptive CERS, and more use of maladaptive CERS. In the final structural equation model (χ2 = 4.82, df = 6, p = 0.57, CFI = 1.0, TLI = 1.10, and RMSEA = 0), the experience of CM and SLE showed a significant indirect effect on NSSI through adaptive CERS. CM and SLE only had direct effects on depression severity.ConclusionsNSSI are prevalent in adolescents and young adults with MDD and highly intertwined with CM, SLE, and CERS. Adaptive CERS, not maladaptive CERS may be a possible mechanism relating CM and SLE to NSSI in MDD patients.
COVID-19 and Acute Cholecystitis Management: A Systematic Review of Current Literature
Authors: Stavridis Konstantinos , Liosis Ioannis , Konstantinidis Michael K. , Kondylis Georgios , Ioannidis Argyrios 
Published: April 2022

IntroductionSince the beginning of the COVID-19 pandemic, many patients with clinically acute presentations have been approached differently. The fear of viral transmission along with the short period of study made patients delay their hospital visits and doctors reassess the approach of certain acute situations. This study aimed to assess the changes in the management of patients with acute cholecystitis before and during COVID-19.MethodsA systematic review of the literature using PubMed (MEDLINE), Scopus, and ScienceDirect databases was performed until 01 September 2021. Totally, two kinds of studies were included, those assessing the management of acute cholecystitis during COVID-19 and those comparing the periods before and during the pandemic. The outcomes recorded include management approaches, complications, and mean length of stay.ResultsA number of 15 eligible articles were included in the study. During the pandemic, six studies revealed a shift toward conservative management of acute cholecystitis and five of them reported that conservative management was opted in 73% of the patients. On the contrary, data from all studies revealed that the surgical approach was preferred in only 29.2% of patients. Furthermore, when comparing the periods before vs. during COVID-19, the conservative approach was reported in 36.3 and 43.2% before vs. during COVID-19, respectively, whereas surgical intervention was performed in 62.5% of patients before COVID-19 and 55.3% during the pandemic. The length of stay was delayed when a non-surgical approach was selected in most studies. Complications, mainly classified by the Clavien-Dindo scale, were higher in the pandemic period.ConclusionA tendency toward more conservative approaches was observed in most studies, reversing the previously used surgical approach in most cases of acute cholecystitis. In most of the examined cases during the COVID-19 pandemic, antibiotic treatment and percutaneous cholecystostomy were much more considered and even preferred.
An Operational Downscaling Method of Solar-Induced Chlorophyll Fluorescence (SIF) for Regional Drought Monitoring
Authors: Hong Zhiming , Hu Yijie , Cui Changlu , Yang Xining , Tao Chongxin , Luo Weiran , Zhang Wen , Li Linyi , Meng Lingkui 
Published: April 2022

Solar-induced chlorophyll fluorescence (SIF) has been shown to be a powerful proxy for photosynthesis and a promising indicator of drought monitoring, but the ability of high-resolution satellite-derived SIF for drought monitoring has not been widely investigated due to a lack of data. The lack of high spatiotemporal resolution satellite SIF hinders the resolution enhancement of SIF derived by downscaling or reconstruction algorithms. The TROPOspheric Monitoring Instrument (TROPOMI) SIF provides an alternative with finer spatiotemporal resolution. We present an operational downscaling method to generate 500 m 16-day SIF (TSIF) using Neural Networks over a local spatiotemporal window. The results showed that our method is very robust against overfitting, and TSIF has a strong spatiotemporal consistency with TROPOMI SIF (TROPOSIF) with R2=0.956 and RMSE=0.054 mWm−2sr−1nm−1. Comparison with another SIF product (CASIF) showed a spatiotemporal consistency with TSIF. Comparison with tower gross primary productivity (GPP) from AmeriFlux in California showed a strong correlation with R2 for multiple ecosystems ranging from 0.58 to 0.88. We explored the capacity of TSIF for monitoring a drought event in Henan, China, showing that TSIF is more sensitive to drought and precipitation compared to the Enhanced Vegetation Index. Our TSIF is a very promising indicator for regional drought monitoring.
Investigation of an Allosteric Deoxyhypusine Synthase Inhibitor in P. falciparum
Authors: Aroonsri Aiyada , Wongsombat Chayaphat , Shaw Philip , Franke Siegrid , Przyborski Jude , Kaiser Annette 
Published: April 2022

The treatment of a variety of protozoal infections, in particular those causing disabling human diseases, is still hampered by a lack of drugs or increasing resistance to registered drugs. However, in recent years, remarkable progress has been achieved to combat neglected tropical diseases by sequencing the parasites’ genomes or the validation of new targets in the parasites by novel genetic manipulation techniques, leading to loss of function. The novel amino acid hypusine is a posttranslational modification (PTM) that occurs in eukaryotic initiation factor 5A (EIF5A) at a specific lysine residue. This modification occurs by two steps catalyzed by deoxyhypusine synthase (dhs) and deoxyhypusine hydroxylase (DOHH) enzymes. dhs from Plasmodium has been validated as a druggable target by small molecules and reverse genetics. Recently, the synthesis of a series of human dhs inhibitors led to 6-bromo-N-(1H-indol-4yl)-1-benzothiophene-2-carboxamide, a potent allosteric inhibitor with an IC50 value of 0.062 µM. We investigated this allosteric dhs inhibitor in Plasmodium. In vitro P. falciparum growth assays showed weak inhibition activity, with IC50 values of 46.1 µM for the Dd2 strain and 51.5 µM for the 3D7 strain, respectively. The antimalarial activity could not be attributed to the targeting of the Pfdhs gene, as shown by chemogenomic profiling with transgenically modified P. falciparum lines. Moreover, in dose-dependent enzymatic assays with purified recombinant P. falciparum dhs protein, only 45% inhibition was observed at an inhibitor dose of 0.4 µM. These data are in agreement with a homology-modeled Pfdhs, suggesting significant structural differences in the allosteric site between the human and parasite enzymes. Virtual screening of the allosteric database identified candidate ligand binding to novel binding pockets identified in P. falciparum dhs, which might foster the development of parasite-specific inhibitors.
Hemoconcentration of Creatinine Minimally Contributes to Changes in Creatinine during the Treatment of Decompensated Heart Failure
Authors: Maulion Christopher , Chen Sheldon , Rao Veena S. , Ivey-Miranda Juan B. , Cox Zachary L. , Mahoney Devin , Coca Steven G. , Negoianu Dan , Asher Jennifer L. , Turner Jeffrey M. , Inker Lesley A. , Wilson F. Perry , Testani Jeffrey M. 
Published: April 2022

Key Points

Hemoconcentration is a minimal contributor to changes in serum creatinine during treatment of decompensated heart failure.Changes in GFR is the primary driver of serum creatinine in treatment of decompensated heart failure.

Background
Worsening serum creatinine is common during treatment of acute decompensated heart failure (ADHF). A possible contributor to creatinine increase is diuresis-induced changes in volume of distribution (VD) of creatinine as total body water (TBW) contracts around a fixed mass of creatinine. Our objective was to better understand the filtration and nonfiltration factors driving change in creatinine during ADHF.

Methods
Participants in the ROSE-AHF trial with baseline to 72-hour serum creatinine; net fluid output; and urinary KIM-1, NGAL, and NAG were included (n=270). Changes in VD were calculated by accounting for measured input and outputs from weight-based calculated TBW. Changes in observed creatinine (Crobserved) were compared with predicted changes in creatinine after accounting for alterations in VD and non–steady state conditions using a kinetic GFR equation (Cr72HR Kinetic).

Results
When considering only change in VD, the median diuresis to elicit a ≥0.3 mg/dl rise in creatinine was −7526 ml (IQR, −5932 to −9149). After accounting for stable creatinine filtration during diuresis, a change in VD alone was insufficient to elicit a ≥0.3 mg/dl rise in creatinine. Larger estimated decreases in VD were paradoxically associated with improvement in Crobserved (r=−0.18, P=0.003). Overall, −3% of the change in eCr72HR Kinetic was attributable to the change in VD. A ≥0.3 mg/dl rise in eCr72HR Kinetic was not associated with worsening of KIM-1, NGAL, NAG, or postdischarge survival (P>0.05 for all).

Conclusions
During ADHF therapy, increases in serum creatinine are driven predominantly by changes in filtration, with minimal contribution from change in VD.
The Modern View on Etiopathogenesis of Vitiligo. Literature Review
Authors: Цепколенко В.А. , Карпенко Е.С. 
Published: April 2022

Витилиго – это хроническое приобретенное нарушение пигментации кожи, характеризующееся наличием резко ограниченных депигментированных участков кожи часто симметричных, что связано с потерей функционирующих эпидермальных меланоцитов и иногда меланоцитов волосяных фолликулов. Встречается примерно в 0,5–2% популяции, однако точную распространенность трудно оценить, так как она отличается в разных географических регионах и среди разных этнических групп. В зависимости от площади, симметричности поражения кожи при витилиго и активности процесса выделяют различные классификации данного заболевания. Витилиго – значимая психологическая и социальная проблема, заболевание может привести к серьезному нарушению качества жизни пациента, особенно если задействованы видимые области тела (лицо, руки). Витилиго является многофакторным заболеванием, которое связано как с генетическими, так и с негенетическими факторами. Патогенез витилиго до настоящего времени остается дискутабельным. Выделяют аутоиммунную теорию, теорию повреждения меланоцитов, теорию оксидативного стресса, а также комбинированную теорию патогенеза витилиго. До настоящего времени многие вопросы этиопатогенеза витилиго остаются недостаточно изученными.
Vitiligo is a chronic acquired disorder of skin pigmentation, characterized by the presence of sharply limited depigmented areas of the skin, often symmetrical, which is associated with the loss of functioning epidermal melanocytes and sometimes melanocytes of hair follicles. It occurs in about 0.5–2% of the population, however, the exact prevalence is difficult to estimate, since it differs in different geographical regions and among different ethnic groups. Depending on the area, the symmetry of skin lesions with vitiligo and the activity of the process, various classifications of this disease are distinguished. Vitiligo is a significant psychological and social problem; a disease can lead to a serious violation of thepatient’s quality of life, especially if visible areas of the body (face, hands) are involved. Vitiligo is a multifactorial disease that is associated with both genetic and non-genetic factors. The pathogenesis of vitiligo is still debatable. The autoimmune theory, the theory of damage to melanocytes, the theory of oxidative stress, as well as the combined theory of the pathogenesis of vitiligo are distinguished. To date, many issues of etiopathogenesis of vitiligo remain insufficiently studied.
Lymphomatoid Papulosis. Clinical Case
Authors: Клименкова Н.В. , Левончук Е.А. , Бич Т.А. , Кузьменко-Москвина Ю.А. , Климова Л.В. 
Published: April 2022

Лимфомы кожи – группа лимфопролиферативных заболеваний Т- и В-клеточной природы, которые первично локализуются только в коже и имеют различные клиническую и морфологическую картины и прогноз. Диагностика лимфом кожи, особенно на ранних стадиях, осложняется клиническим и гистологическим совпадением с другими доброкачественными воспалительными дерматозами. Оценка дерматоскопической картины заболевания может быть полезна для дифференциальной диагностики с воспалительными дерматозами и другими лимфопролиферативными заболеваниями кожи. Лимфоматоидный папулез относится к группе первичных кожных CD30+ лимфопролиферативных заболеваний, второй по частоте возникновения после грибовидного микоза и составляющей до 25% всех первичных лимфом кожи. Лимфоматоидный папулез характеризуется относительно доброкачественным течением и тенденцией к самопроизвольному разрешению элементов.В статье представлены данные клинической картины, морфологических, гистологических и иммуногистохимических характеристик лимфоматоидного папулеза кожи. Приведено собственное наблюдение клинического случая лимфоматоидного папулеза. Описаны дерматоскопические признаки данного заболевания. Представлены фотографии клинического, дерматоскопического, гистологического и иммуногистохимического исследований.
Skin lymphomas are a group of lymphoproliferative diseases of T- and B-cell nature, which are primarily localized only in the skin and have different clinical and morphological patterns and prognosis. Diagnosis of skin lymphomas, especially in the early stages, is complicated because of clinical and histological coincidence with other benign inflammatory dermatoses. Evaluation of the dermatoscopic picture of the disease may be useful for the differential diagnosis with inflammatory dermatoses and other lymphoproliferative skin diseases. Lymphomatoid papulosis belongs to the group of primaryskin CD30+ lymphoproliferative diseases. It appears to be the second most common after fungal mycosis and up to 25% of all primary skin lymphomas. Lymphomatoid papulosis is characterized by a relatively benign course and a tendency to spontaneous resolution of elements.The article presents the data of the clinical picture, morphological, histological, and immunohistochemical characteristics of lymphomatoid skin papulosis. Own observation of the clinical case of lymphomatoid papulosis is given. Dermatoscopic signs of this disease are described. Photographs of clinical, dermatoscopic, histological, and immunohistochemical studies are presented.
The Restoration of the Blood Service in Belarus in 1944–1945 (to the 75th Anniversary of the Victory in the Great Patriotic War)
Authors: Гольдинберг Б.М. , Климович О.В. 
Published: April 2022

В статье рассмотрены особенности начала восстановления службы крови Беларуси по мере освобождения территории республики от немецко-фашистских захватчиков и вплоть до окончания Великой Отечественной войны. Показано, какими усилиями выполнялась основная задача, поставленная Государственным комитетом обороны СССР и правительством республики, – заготовка крови для нужд фронтовых и тыловых госпиталей.
In the article, there are considered the peculiarities of the beginning of the restoration of the Blood Service in Belarus as the territory of the Republic was liberated from the German-fascist invaders and until the end of the Great Patriotic war. It was showed what efforts were made to fulfill the main task set by the State Defense Committee of the USSR and the government of the Republic – blood preservation for the needs of front and rear hospitals.