bluuebunny/crossref_metadata_2025_split huggingface dataset:
Method for full magnetic gradient tensor detection from a single HTS gradiometer
Authors: Keenan S T , Clark D A , Leslie K E 
Published: January 2022

Abstract
A new method to measure the full magnetic gradient tensor (FMGT) using a single rotating high-temperature superconducting planar first-order gradiometer is described. Determining the FMGT provides valuable additional information about magnetic sources and their anomalies and, critically, the location of magnetic targets with less ambiguity and with fewer survey lines required. This paper presents both a theoretical description and experimental demonstration of the single sensor system that can, in principle, extract all the magnetic gradient tensor components, with suitable referencing and/or field cancellation procedures. The technique involves rotating the planar gradiometer mounted on an angled plinth about the vertical axis of the sensor package. The technique works by shifting gradient terms to harmonics of the rotating frequency, above the 1/f noise of the device, and allows extraction of all the components in the frequency domain. Additional referencing enables compensation for the non-ideal gradiometer response and provides a more accurate measurement of contaminated gradient components. Laboratory results show tracking of a moving dipole target and these are compared to a modelled response, both with and without field compensation.
Investigation of self-generated magnetic field and dynamics of a pulsed plasma flow
Authors: TAZHEN Aigerim , DOSBOLAYEV Merlan , RAMAZANOV Tlekkabul 
Published: January 2022

Abstract
Due to the growing interest in studying the compression and disruption of the plasma filament in magnetic fusion devices and Z-pinches, this work may be important for new developments in the field of controlled thermonuclear fusion. Recently, on a coaxial plasma accelerator, we managed to obtain the relatively long-lived (∼300 μs) plasma filaments with its self-magnetic field. This was achieved after modification of the experimental setup by using high-capacitive and low-inductive energy storage capacitor banks, as well as electrical cables with low reactive impedance. Furthermore, we were able to avoid the reverse reflection of the plasma flux from the end of the plasma accelerator by installing a special plasma-absorbing target. Thus, these constructive changes of the experimental setup allowed us to investigate the physical properties of the plasma filament by using the comprehensive diagnostics including Rogowski coil, magnetic probes, and Faraday cup. As a result, such important plasma parameters as density of ions and temperature of electrons in plasma flux, time dependent plasma filament’s azimuthal magnetic field were measured in discharge gap and at a distance of 23.5 cm from the tip of the cathode. In addition, the current oscillograms and I–V characteristics of the plasma accelerator were obtained. In the experiments, we also observed the charge separation during the acceleration of plasma flow via oscillograms of electron and ion beam currents.
Evaluation of a Beta-Blocker–Edema–Loop Diuretic Prescribing Cascade: A Prescription Sequence Symmetry Analysis
Authors: Vouri Scott Martin , Morris Earl J , Jiang Xinyi , Hofer Ann-Kathrin , Schmidt Stephan , Pepine Carl , Winterstein Almut G , Smith Steven M 
Published: January 2022

Abstract

BACKGROUND
Drug-related adverse events associated with antihypertensive therapy may result in subsequent prescribing of other potentially harmful medications, known as prescribing cascades. The aim of this study was to assess the magnitude and characteristics of a beta-blocker–edema–loop diuretic prescribing cascade.

METHODS
A prescription sequence symmetry analysis was used to assess loop diuretic initiation before and after initiation of beta-blockers among patients 20 years or older without heart failure, atrial fibrillation, other arrythmias, or use of calcium channel blocker within a U.S. private insurance claims database (2005–2018). The temporality of loop diuretic initiation relative to a beta-blocker or negative control (renin-angiotensin system blocker) initiation was tabulated. Secular trend-adjusted sequence ratios (aSRs) with 95% confidence intervals (CIs) compared the initiation of loop diuretic 90 days before and after initiation of beta-blockers.

RESULTS
Among 988,675 beta-blocker initiators, 9,489 patients initiated a new loop diuretic prescription 90 days after and 5,245 patients before beta-blocker initiation, resulting in an aSR of 1.78 (95% CI, 1.72–1.84). An estimated 1.72 beta-blocker initiators per 100 patient-years experienced the prescribing cascade in the first 90 days. The aSR was disproportionately higher among older adults (aSR 1.97), men (aSR 2.25), and patients who initiated metoprolol tartrate (aSR 2.48), labetalol (aSR 2.18), or metoprolol succinate (aSR 2.11). Negative control results (aSR 1.09, 95% CI, 1.05–1.13) generally corroborated our findings, but suggested modest within-person time-varying confounding.

CONCLUSIONS
We observed excess use of loop diuretics following beta-blocker initiation that was only partially explained by secular trends or hypertension progression.
A fungal protease named AsES triggers antiviral immune responses and effectively restricts virus infection in arabidopsis andNicotiana benthamianaplants
Authors: Caro Maria Del Pilar , Venturuzzi Andrea Laura , Moschen Sebastian , Salazar Sergio Miguel , Díaz-Ricci Juan Carlos , Asurmendi Sebastian 
Published: January 2022

AbstractBackground and AimsPlants have evolved complex mechanisms to fight against pathogens. Among these mechanisms, pattern-triggered immunity (PTI) relies on the recognition of conserved microbe- or pathogen-associated molecular patterns (MAMPs or PAMPs, respectively) by membrane-bound receptors. Indeed, PTI restricts virus infection in plants and, in addition, BRI1-associated kinase 1 (BAK1), a central regulator of PTI, plays a role in antiviral resistance. However, the compounds that trigger antiviral defences, along with their molecular mechanisms of action, remain mostly elusive. Herein, we explore the role of a fungal extracellular subtilase named AsES in its capacity to trigger antiviral responses.MethodsIn this study, we obtained AsES by recombinant expression, and evaluated and characterized its capacity to trigger antiviral responses against Tobacco mosaic virus (TMV) by performing time course experiments, analysing gene expression, virus movement and callose deposition.Key ResultsThe results of this study provide direct evidence that exogenous treatment with recombinant AsES increases a state of resistance against TMV infection, in both arabidopsis and Nicotiana benthamiana plants. Also, the antiviral PTI response exhibited by AsES in arabidopsis is mediated by the BAK1/SERK3 and BKK1/SERK4 co-receptors. Moreover, AsES requires a fully active salicylic acid (SA) signalling pathway to restrict the TMV movement by inducing callose deposition. Additionally, treatment with PSP1, a biostimulant based on AsES as the active compound, showed an increased resistance against TMV in N. benthamiana and tobacco plants.ConclusionsAsES is a fungal serine protease which triggers antiviral responses relying on a conserved mechanism by means of the SA signalling pathway and could be exploited as an effective and sustainable biotechnology strategy for viral disease management in plants.
Effect of high-dose 290 nm UV-B on resveratrol content in grape skins
Authors: Tsurumoto Tomohiro , Fujikawa Yasuo , Onoda Yushi , Kamimori Masahiro , Hiramatsu Kazuya , Tanimoto Hideo , Ohta Daisaku , Okazawa Atsushi 
Published: January 2022

ABSTRACTUV-C irradiation increases resveratrol content in grape skins, but it reaches a maximum at a certain UV-C dose. In contrast, UV-B has a weak resveratrol-enhancing effect at low doses, but it has not been investigated at high doses. In this study, we investigated the effect of high-dose UV-B on resveratrol contents in grape skins. Irradiation of Muscat Bailey A with 290 nm UV-B LED at 22 500 and 225 000 µmol m−2 increased the resveratrol contents in the grape skins by 2.1- and 9.0-fold, respectively, without significant increases in other phenolic compounds. The effect was also confirmed for 2 other cultivars: Shine Muscat and Delaware. Transcriptome analysis of the grape skins of Muscat Bailey A immediately after irradiation with UV-B at 225 000 µmol m−2 showed that genes related to biotic and abiotic stresses were upregulated. Hence, it was suggested that high-dose UV-B irradiation induces a stress response and specifically activates resveratrol biosynthesis.
Crystal structures of glycoside hydrolase family 136 lacto-N-biosidases from monkey gut- and human adult gut bacteria
Authors: Yamada Chihaya , Katayama Takane , Fushinobu Shinya 
Published: January 2022

ABSTRACT
Glycoside hydrolase family 136 (GH136) was established after the discovery and structural analysis of lacto-N-biosidase (LNBase) from the infant gut bacterium Bifidobacterium longum subsp. longum JCM1217 (BlLnbX). Homologous genes of BlLnbX are widely distributed in the genomes of human gut bacteria and monkey Bifidobacterium spp., although only 2 crystal structures were reported in the GH136 family. Cell suspensions of Bifidobacterium saguini, Tyzzerella nexilis, and Ruminococcus lactaris exhibited the LNBase activity. Recombinant LNBases of these 3 species were functionally expressed with their specific chaperones in Escherichia coli, and their kinetic parameters against p-nitrophenol substrates were determined. The crystal structures of the LNBases from B. saguini and T. nexilis in complex with lacto-N-biose I were determined at 2.51 and 1.92 Å resolutions, respectively. These structures conserve a β-helix fold characteristic of GH136 and the catalytic residues, but they lack the metal ions that were present in BlLnbX.
Habitat determines convergent evolution of cephalic horns in vipers
Authors: Busschau Theo , Boissinot Stephane 
Published: January 2022

AbstractPhenotypic convergence of traits in similar environments can provide insights into the evolutionary processes shaping trait evolution. Among squamate reptiles, horn-like cephalic appendages have evolved under various selective pressures, including selection for defence, crypsis or sexual selection. Yet, among snakes, particularly vipers, the functional and evolutionary significance of horns are unknown. We used a comparative phylogenetic approach with habitat and diet data on 263 viper taxa to shed light on the selective pressures underlying horn evolution in vipers. We detected significant correlations with habitat but not diet. The relative positions of horns are ecologically divergent in that supranasal horns are positively correlated with terrestrial forest habitats while supraocular horns are negatively correlated with terrestrial forest habitats and are instead associated with arboreal or sparsely vegetated habitats. Multiple independent origins of supranasal or supraocular horns in similar habitats provide evidence of adaptive convergence. Comparisons with other snake lineages suggest that cephalic appendages may have evolved under selection for crypsis in ambush foraging snakes.
Effect of the learning curve on survival after laparoscopic liver resection for colorectal metastases
Authors: Shanti Hiba , Raman Rakesh , Chakravartty Saurav , Belgaumkar Ajay P. , Patel Ameet G. 
Published: January 2022

Abstract

Background
Laparoscopic liver resection (LLR) is a highly demanding procedure with great variability. Previously published randomized trials have proven oncological safety of laparoscopic liver resection (LLR) as compared to open surgery. However, these were started after the learning curve (LC) was established. This leaves the question of whether the LC of LLR in the early laparoscopic era has affected the survival of patients with colorectal liver metastasis (CRLM).

Methods
All consecutive LLRs performed by a single surgeon between 2000 and 2019 were retrospectively analysed. A risk-adjusted cumulative sum (RA-CUSUM) chart for conversion rate and the log regression analysis of the blood loss identified two phases in the LC. This was then applied to patients with CRLM, and the two subgroups were compared for recurrence-free (RFS) and overall survival (OS). The analysis was repeated with propensity score-matched (PSM) groups

Results
A total of 286 patients were included in the LC analysis, which identified two distinct phases, the early (EP; 68 patients) and the late (LP; 218 patients) phases. The LC was applied to 192 patients with colorectal liver metastasis (EPc, 45 patients; LPc, 147 patients). For patients with CRLM, R0 resection was achieved in 93 per cent: 100 per cent in the EPc group and 90 per cent in the LPc group (P = 0.026). Median OS and RFS were 60 and 16 months, respectively. The 5-year OS and RFS were 51 per cent and 32.7 per cent, respectively. OS (hazard ratio (h.r.) 0.78, 95 per cent confidence interval (c.i.) 0.51 to 1.2; P = 0.286) and RFS (h.r. 0.94, 95 per cent c.i. 0.64 to 1.37; P = 0.760) were not compromised by the learning curve. The results were replicated after PSM.

Conclusion
In our experience, the development of a laparoscopic liver resection programme can be achieved without adverse effects on the long-term survival of patients with CRLM.
Coffee consumption and its interaction with the genetic variant AhR rs2066853 in colorectal cancer risk: a case–control study in Korea
Authors: Lu Y-Thanh , Gunathilake Madhawa , Lee Jeonghee , Kim Youngyo , Oh Jae Hwan , Chang Hee Jin , Sohn Dae Kyung , Shin Aesun , Kim Jeongseon 
Published: January 2022

Abstract
The bioactive compounds in coffee have several antioxidant properties that may beneficially impact colorectal cancer (CRC) development. The aryl hydrocarbon receptor (AhR) is an important transcription factor that regulates an enzyme related to the caffeine metabolism pathway. We investigated the modification effect on coffee of AhR gene polymorphism in the risk of CRC. A case–control study was conducted with 699 cases and 1393 controls to investigate the interaction between coffee intake and the AhR rs2066853 variant in CRC risk. The odds ratios (ORs) and 95% confidence intervals (CIs) were assessed using multiple logistic regression analyses. We observed a significant protective effect of coffee against CRC in the overall and male populations. Consuming three or more cups of coffee per day may significantly lower CRC risk in all subjects by 77% and in men by 83% (OR = 0.23, 95% CI: 0.14–0.39 and OR = 0.17, 95% CI: 0.09–0.34, respectively, P-trends &lt; 0.001). No association between AhR rs2066853 and CRC risk was found. In the dominant model, the G/G genotype had a strongest synergistic effect with coffee on protection against CRC (OR = 0.12, 95% CI: 0.06–0.26, P-interaction = 0.014). The interaction remained significant in men and the distal colon cancer subgroup. In the additive model, the interaction was clearly shown strongest in G/G carriers (OR = 0.12, 95% CI: 0.06–0.27, P-interaction = 0.039), followed by A/A and G/A carriers. The interaction remained significant in men and the rectal cancer subgroup. In conclusion, the protective effect of coffee on CRC risk might interact with the genetic variant AhR rs2066853, and this joint effect was determined by sex and site-specific cancer.
Prefrontal cortical distribution of muscarinic M2 and cannabinoid-1 (CB1) receptors in adult male mice with or without chronic adolescent exposure to Δ9-tetrahydrocannabinol
Authors: Garzón Miguel , Chan June , Mackie Ken , Pickel Virginia M 
Published: January 2022

Abstract
Chronic adolescent administration of marijuana’s major psychoactive compound, ∆9-tetrahydrocannabinol (Δ9-THC), produces adaptive changes in adult social and cognitive functions sustained by prelimbic prefrontal cortex (PL-PFC). Memory and learning processes in PL-PFC neurons can be regulated through cholinergic muscarinic-2 receptors (M2R) and modulated by activation of cannabinoid-1 receptors (CB1Rs) targeted by Δ9-THC. Thus, chronic exposure to Δ9-THC during adolescence may alter the expression and/or distribution of M2Rs in PL-PFC neurons receiving CB1R terminals. We tested this hypothesis by using electron microscopic dual CB1R and M2R immunolabeling in adult C57BL/6 J male mice that had received vehicle or escalating dose of Δ9-THC through adolescence. In vehicle controls, CB1R immunolabeling was mainly localized to axonal profiles virtually devoid of M2R but often apposing M2R-immunoreactive dendrites and dendritic spines. The dendrites received inputs from CB1R-labeled or unlabeled terminals, whereas spines received asymmetric synapses exclusively from axon terminals lacking CB1Rs. Adolescent Δ9-THC significantly increased plasmalemmal M2R-immunogold density exclusively in large dendrites receiving input from CB1R-labeled terminals. In contrast, cytoplasmic M2R-immunogold density decreased in small spines of the Δ9-THC-treated adult mice. We conclude that Δ9-THC engagement of CB1Rs during adolescence increases M2R plasmalemmal accumulation in large proximal dendrites and decreases M2R cytoplasmic expression in small spines of PL-PFC.
Is a Mummy a Person or a Property: The Classification and Choice of Law of Cultural Objects in Private International Law
Authors: Chen Zhen 
Published: January 2022

Abstract
In the Buddha Mummy Statue case, the Chinese village committees sued the Dutch defendants for the return of a stolen golden statue that contains a mummified Buddha. The parties had different opinions on the legal nature of the mummy contained in the statue. The Chinese court classified the statue as a cultural property and applied the choice of law over movable properties provided in Article 37 of the Chinese Private International Law Act (lex rei sitae). Based on a comparative study, this article argues that a mummy does not fall within the traditional dichotomy between a person and a property. Instead, a mummy should be classified as a transitional existence between a person and a property. If the classification of a mummy has to be confined to the traditional dichotomy, a mummy can be regarded as a quasi-person or a special kind of property. Following this new classification, a new choice-of-law rule should be established. In this regard, the Belgian Private International Law Act, which adopts the lex originis rule, supplemented by the lex rei sitae rule, is a forerunner. This article advocates that the adoption of the lex originis rule may help to stop the vicious circle of illegal possession of stolen cultural objects and facilitate the return of stolen cultural objects, especially those containing human remains, to their country of origin.
Contributing factors to lymph node recovery with esophagectomy by thoracic surgeons: an analysis of the Society of Thoracic Surgeons General Thoracic Surgery Database
Authors: Sachdeva Uma M , Axtell Andrea L , Kroese Tiuri E , Chang David C , Mathisen Douglas J , Morse Christopher R 
Published: January 2022

Abstract
Given the association between lymphadenectomy and survival after esophagectomy, and the ongoing development of effective adjuvant protocols for identified residual disease, we determined factors contributing to lymph node yield and effects on postoperative morbidity following esophagectomy by thoracic surgeons. Using the Society of Thoracic Surgeons General Thoracic Surgery Database, all patients who underwent esophagectomy for primary esophageal cancer with gastric conduit reconstruction from 2012 to 2016 were identified. Patient demographics, technical factors, and tumor characteristics associated with lymph node yield were determined using a multivariable multilevel mixed-effects regression model. Associations between lymph node yield and perioperative morbidity and mortality were similarly assessed. A total of 8480 patients were included. The median number of nodes harvested was 16 [Interquartile Range 11–22]. Factors associated with fewer nodes included female gender (b=−0.53, P=0.032), body mass index &lt;18.5 (b=−1.46, P=0.012), prior cardiothoracic surgery (b=−0.73, P=0.015), intraoperative blood transfusion (b=−1.43, P&lt;0.001), squamous cell histology (b=−0.86, P=0.006), and neoadjuvant treatment (b=−1.41, P&lt;0.001). Operative approach significantly affected lymph node yield, with minimally invasive approaches demonstrating higher lymph node counts, and open transhiatal esophagectomy recovering the fewest nodes. Findings were independent of clinical center. There was no association of higher lymph node yield with 30-day mortality, with only slightly increased risk for chyle leak (odds ratio [OR] 1.02, P=0.012). In conclusion, several patient and tumor factors affect lymph node recovery with esophagectomy, independent of hospital center. Technical aspects, specifically minimally invasive approach, play a significant role in quantified lymph node yield. Higher operative lymph node yield was associated with minimal increased morbidity.
Estimating the SARS-CoV-2 infection fatality rate by data combination: the case of Germany’s first wave
Authors: Dimpfl Thomas , Sönksen Jantje , Bechmann Ingo , Grammig Joachim 
Published: January 2022

Summary
Assessing the infection fatality rate (IFR) of SARS-CoV-2 in a population is a controversial issue. Due to asymptomatic courses of COVID-19, many infections remain undetected. Reported case fatality rates are therefore poor estimates of the IFR. We propose a strategy to estimate the IFR that combines official data on cases and fatalities with data from seroepidemiological studies in infection hotspots. The application of the method yields an estimate of the IFR of wild-type SARS-CoV-2 in Germany during the first wave of the pandemic of 0.83% (95% CI: [0.69%; 0.98%]), notably higher than the estimate reported in the prominent study by Streeck et al. (2020) (0.36% [0.17%; 0.77%]) and closer to that obtained from a world-wide meta analysis (0.68% [0.53%; 0.82%]), where the difference can be explained by Germany’s disadvantageous age structure. Provided that suitable data are available, the proposed method can be applied to estimate the IFR of virus variants and other regions.
Misclassification-robust semiparametric estimation of single-index binary-choice models
Authors: Čížek P , Sadıkoğlu S 
Published: January 2022

Summary
In this paper, a new class of semiparametric estimators for single-index binary-choice models is introduced. The proposed estimators are based on the semiparametric indirect inference that identifies and estimates the parameters of the model via possibly misspecified auxiliary criteria. A large class of considered auxiliary criteria includes the ordinary least squares, nonlinear least squares, and nonlinear least absolute deviations estimators. Besides deriving the consistency and asymptotic normality of the proposed methods, we demonstrate that the proposed indirect inference methodology—at least for selected auxiliary criteria—combines weak distributional assumptions, good estimation precision, and robustness to misclassification of responses. We conduct Monte Carlo experiments and an application study to compare the finite-sample performance of the proposed and existing estimators.