bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel and dual digestive symbiosis scales up the nutrition and immune system of the holobiont Rimicaris exoculata
Authors: Aubé Johanne , Cambon-Bonavita Marie-Anne , Velo-Suárez Lourdes , Cueff-Gauchard Valérie , Lesongeur Françoise , Guéganton Marion , Durand Lucile , Reveillaud Julie 
Published: April 2022

Abstract
Background
In deep-sea hydrothermal vent areas, deprived of light, most animals rely on chemosynthetic symbionts for their nutrition. These symbionts may be located on their cuticle, inside modified organs, or in specialized cells. Nonetheless, many of these animals have an open and functional digestive tract. The vent shrimp Rimicaris exoculata is fueled mainly by its gill chamber symbionts, but also has a complete digestive system with symbionts. These are found in the shrimp foregut and midgut, but their roles remain unknown. We used genome-resolved metagenomics on separate foregut and midgut samples, taken from specimens living at three contrasted sites along the Mid-Atlantic Ridge (TAG, Rainbow, and Snake Pit) to reveal their genetic potential.
Results
We reconstructed and studied 20 Metagenome-Assembled Genomes (MAGs), including novel lineages of Hepatoplasmataceae and Deferribacteres, abundant in the shrimp foregut and midgut, respectively. Although the former showed streamlined reduced genomes capable of using mostly broken-down complex molecules, Deferribacteres showed the ability to degrade complex polymers, synthesize vitamins, and encode numerous flagellar and chemotaxis genes for host-symbiont sensing. Both symbionts harbor a diverse set of immune system genes favoring holobiont defense. In addition, Deferribacteres were observed to particularly colonize the bacteria-free ectoperitrophic space, in direct contact with the host, elongating but not dividing despite possessing the complete genetic machinery necessary for this.
Conclusion
Overall, these data suggest that these digestive symbionts have key communication and defense roles, which contribute to the overall fitness of the Rimicaris holobiont.
Education as a homogenizer engine in indigenous cultures: Aymara children in northern Chile´s case
Authors: Paulsen Abraham , Rivera Victoria 
Published: April 2022

Abstract
Chile is a multi-ethnic republic; situation which contradicts itself when one looks at the relationship between the State and its ethnic groups, such as the Aymara in the North. One example of this is the implementation of a state-centric educational model which is homogenizing. Taking a Children’s Geographies approach, the following work will analyse the specific types of relationship in educational institutions located in the Arica & Parinacota Region, between children from Aymara communities, the State and adults. Through different qualitative investigative techniques, such as content analysis of different instruments, it was possible to verify that the educational process which the Aymara children experience does not consider their culture, which sets them apart from their parents and community’s identity. This study also uses the lens of Children’s Geographies to identify some landmarks in their spatial worldview transformation and their spatiality. Lastly, some conclusions will be stated, on how the normative educational model causes uprooted children.
Digitally prefabricated versus conventionally fabricated implant-supported full-arch provisional prosthesis: A retrospective cohort study
Authors: Chen Chaoqun , Lai Haiyan , Zhu Huiyong , Gu Xinhua 
Published: April 2022

Abstract
Background: To evaluate and compare the clinical outcomes of digitally prefabricated and conventionally fabricated implant-supported full-arch provisional prostheses.Methods: Thirty-nine patients who underwent implant-supported full-arch rehabilitation were included in this study. Implant-supported provisional prostheses were digitally prefabricated before implant surgery in group A (20 patients), while implant-supported provisional prostheses were conventionally fabricated immediately after surgery in group B (19 patients). Implant/prosthesis survival rate and complications were recorded. Marginal bone loss（MBL）was investigated by CBCT. Surgical time, restorative time, and total operative time were analyzed. Postoperative pain and swelling were evaluated with visual analog scale (VAS). The Oral Health Impact Profile (OHIP) questionnaire was administered before and after surgery.Results: The implant/prosthesis survival rate was 100% and complications appeared with low frequency in both group, while the mean MBL was 0.30±0.29 mm in group A and 0.31±0.41 mm in group B after 3~6 months(P>0.05). The average restorative time in group A (116.16±16.61 mins) was significantly shorter than that in group B (242.11±30.14 mins) (P<0.05). Patients in group A showed lower pain/swelling VAS scores after surgery than group B (P<0.05). Low OHIP scores with high satisfaction to the overall effects were showed in both groups. Conclusions: Prefabricated prosthesis could reduce prosthetic time and postoperative discomfort in patient whose immediate rehabilitation was based on All-on-4 concept. The prefabrication technology could be a predictable alternative to improve the short-term clinical outcome of implant-supported full-arch provisional rehabilitation.
Comparison of Quality of Life Questionnaires, in Patients With OSAS
Authors: FREITAS GABRIEL Santos , PINTO JOSÉ ANTONIO , RIBEIRO DAVI KNOL , Nunes Heloisa Sobreira 
Published: April 2022

Abstract
INTRODUCTION: Self-assessment questionnaires, can provide subjective data about the intensity of daytime sleepiness. Among the existing questionnaires the most used one is the Epworth Sleepiness scale (ESS), which measures the propensity to fall asleep, as well as the severity of excessive sleepiness, [5] and the evaluation of the response in OSA treatment. In 1997, the Functional Outcome Sleep Questionnaire (FOSQ), was developed to evaluate the functional impact of sleepiness in daily life activities [6]. In this study, we will use the Epworth sleepiness scale, and the Functional Outcome Sleep Questionnaire in its shortest version, known as FOSQ 10[7], developed in 2009 by Weaver, who in addition to drowsiness included the relationship of sleep to the quality of life. MATERIALS AND METHODS: The following variables were analyzed: age, anthropometric characteristics (weight, height, body mass index [BMI]), duration of complaints, type of complaints related to sleep (snoring, insomnia, excessive daytime sleepiness), sleep apnea and hypopnea index (AHI). Inclusion criteria were patients between 18 and 80 years, without prior surgery, with complaints related to sleep disorders. Exclusion criteria were: children under 18 years, previous surgeries, previous use of CPAP. RESULTS: the significance level at 5%, where we observed moderate and significant inverse correlation between FOSQ and ESS (p < 0.001), which evidences that the higher the ESS value, the lower the FOSQ value will be CONCLUSION: This study has shown an acceptable correlation between the scores on the Epworth and FOSQ scale
COVID-19 Prevention Guidance and the Incidence of Febrile Neutropenia in Breast Cancer Patients Receiving TAC Chemotherapy with Prophylactic Pegfilgrastim
Authors: Gwak Hongki , Lim Seung-Taek , Jeon Ye-Won , Suh Young-Jin J. 
Published: April 2022

Abstract
PurposeChemotherapy-induced febrile neutropenia (FN) is a medical emergency that can cause severe adverse effects and death. Respiratory infection is one of the main causes of FN. We studied whether infection prevention and control (IPC) guidance for COVID-19 reduces the incidence of FN.MethodsWe reviewed female breast cancer patients treated with adjuvant docetaxel, doxorubicin, and cyclophosphamide (TAC) with prophylactic pegfilgrastim between 2019 and 2021. IPC guidance has been implemented since April 2020.ResultsThere was no difference in the incidence of chemotherapy-induced neutropenia (CIN) between patients without and with IPC. In the IPC patients, the incidence of FN (9.5%), hospitalization duration (0.7 ± 1.5 days), and total hospital cost (279.6 ± 42.6 USD) were significantly lower than in non-IPC patients (27.9%, 2.0 ± 3.8 days and 364.7 ± 271.6 USD, respectively).ConclusionIPC guidance should be implemented to prevent FN in high-risk patients.
Effects of inhaled nitric oxide (iNO) in pulmonary hypertension secondary to arteriovenous malformations: a retrospective cohort study from the European iNO Registry
Authors: Chakkarapani Aravanan Anbu , Gupta Samir , Jamil Asma , Yadav Santosh Kumar , Subhedar Nim , Hummler Helmut D 
Published: April 2022

Abstract
Purpose: To assess the effect of inhaled nitric oxide (iNO) on oxygenation in the management of pulmonary hypertension (PH) secondary to arteriovenous malformations (AVMs) in neonates.Method: A matched retrospective cohort study from January 1, 2013, to December 31, 2017. European inhaled nitric oxide registry from 43 neonatal and pediatric ICUs in 13 countries across Europe was used to extract data. The target population was neonates treated with iNO for the management of PH. The cases (PH secondary to AVMs treated with iNO) were matched (1:4 ratio) to controls (PH without AVMs treated with iNO). The main outcome measure was the absolute change of oxygenation index (OI) from baseline to 60 minutes after starting iNO in cases and control.Results: A total of 45 infants out of 1830 (9 cases and 36 controls) were identified, who received inhaled nitric oxide during ICU stay. The mean baseline value of OI (%) (cases: 27.5, controls: 34), the mean value of OI (%) after 60 minutes of iNO use (cases: 16.8, controls: 28.5), and the mean absolute change in OI value (%) from baseline to 60 minutes of iNO (cases: 10.7 and controls: 6) was not statistically different between groups.Conclusion: Infants with PH secondary to AVMs treated with iNO did not respond differently compared to babies presented with PH without AVMs treated with iNO. Right ventricle dysfunction on echocardiography was higher in cases (cases: 66.7% and controls: 28.6%) but not statistically significant. The death rate before discharge was significantly higher among cases (cases: 55.6% and controls: 8.3%).
Nanorod-Like Nanocrystalline CsSnI3 and CNTs Composite Thin Film-Based Hybrid Photodetector
Authors: Howlader Ashraful Hossain , Li Feng , Zheng Rongkun 
Published: April 2022

Abstract
An experimental detail on the morphology engineering and characterizations of the all-inorganic Sn-based perovskite (here CsSnI3) thin films and their application in photodetectors are presented. In particular, we demonstrated that the chlorobenzene anti-solvent treatment during thin film spin-coating could effectively optimize the morphology properties of the obtained CsSnI3 thin film. SEM and AFM measurements showed the uniform thin film with nanorod-like nanocrystalline morphology. In addition, EDS and XPS measurements confirmed the low level of oxidation of the thin film, indicating good ambient stability. A planar photodetector was also made with the prepared thin film, and electrical characteristics were taken. The dark current and photocurrent were found in the range of 10-9 A and 10-7 A, respectively, with an on/off ratio of 102. The photoresponsivity was 10-5 AW-1. A further experiment was conducted to make composite thin films between CsSnI3 and CNTs for additional morphological engineering. The SEM measurement and Raman mapping manifested the nanonet-like morphology of the composite thin film. The quenching of the PL curve indicated the efficient photo-generated carrier extraction from the CsSnI3 matrix to CNTs. The absorption spectra also showed enhanced absorption ability of the prepared composite thin film. A hybrid photodetector made from the composite thin film showed dark current and photocurrent in the range of 10-6 A and 10-4 A, respectively, with an on/off ratio of 102. The photoresponsivity was 10-2 AW-1. Due to the combination of the CNTs with CsSnI3, The photoresponsivity increased 1000 times. At the same time, the hysteresis of the hybrid photodetector also reduced significantly compared to pristine CsSnI3-based photodetector.
Efficient development of gait classification models for five-gaited horses
Authors: Davíðsson Haraldur B. , Rees Torben , Ólafsdóttir Marta Rut , Einarsson Hafsteinn 
Published: April 2022

Abstract
In this study, we perform horse gait classification using mobile phone sensors located in the rider’s pocket. We train a classifier using data obtained simultaneously from a mobile phone and a gait label acquired from a device based on four wearable sensors attached to a horse’s limbs. With this efficient approach to acquire labelled data, we train a gait classification model and demonstrate that data from mobile phones can be used to classify the five gaits of the Icelandic horse with up to 98% accuracy. The result suggests that horseriding activities can be studied at a large scale using mobile phones to gather data on gaits.
Correlation between carpal rotational alignment and postoperative wrist range of motion following total wrist arthroplasty
Authors: Ota Mitsutoshi , Matsui Yuichiro , Kawamura Daisuke , Urita Atsushi , Endo Takeshi , Iwasaki Norimasa 
Published: April 2022

Abstract
Background: Although total wrist arthroplasty (TWA) has become a common treatment option for wrists with damage due to rheumatoid arthritis (RA), the optimal implant axial alignment for TWA has been inadequately studied. This study was performed to investigate the relationships between implant alignment and carpal rotational alignment and the wrist range of motion (ROM) following TWA.Methods: This study included 18 patients who underwent TWA using a DARTS® Total Wrist System (Teijin Nakashima Medical, Okayama, Japan) for wrist RA. Pre- and 6-month postoperative computed tomography scans were performed, including the radial volar line (Rv), capitohamate axis (CH), and Rv-CH angle in axial scans. The wrist ROM was also measured. The relationship between the Rv-CH angle and ROM was examined.Results: The mean Rv-CH angle showed significant wrist pronation from 73.0° to 83.4° postoperatively. We observed a significant positive correlation (0.58) between the postoperative Rv-CH angle and extension and a significant negative correlation (−0.56) between the postoperative Rv-CH angle and flexion.Conclusions: Implantation of the DARTS® TWA prosthesis resulted in pronation of the carpal axial alignment, which was correlated with postoperative wrist extension. The volar cortex of the distal radius can be a novel reference axis for adequate implant placement.
Overexpression of Dock180 and Elmo1 in melanoma is associated with cell survival and migration
Authors: Lee Yoon Jin , Choi Yu Sung , Kim Sooyoung , Heo Jae Young , Kim Dong Sung , Nam Seung Min , Nam Hae Seon , Lee Sang Han , Choi Dongsic , Cho Moon Kyun 
Published: April 2022

Abstract
Melanoma is one of the most aggressive and metastatic skin cancers, of these metastatic melanoma is a lethal disease with invasive behavior. Although overexpression of Dock180 and Elmo1 has been identified in various cancers, including glioma, ovarian cancer, hepatocellular carcinoma, and breast cancer, the expression and functions of them in melanoma remain unknown. Therefore, the purpose of this study is to confirm the expression of Dock180 and Elmo1, their underlying mechanisms, and roles in melanoma. Both immunohistochemical staining and Western blotting were used to confirm expression of Dock180 and Elmo1 in human melanoma and normal skin. To identify roles of Dock180 and Elmo1 in cell survival, apoptosis and migration, downregulation of Dock180 or Elmo1 in melanoma cells treated with siRNA was performed in Cell viability assay, Phase contrast images, DAPI staining, Cell cycle analysis, Apoptosis assay, Wound healing assay, Colony formation assay, and Western blotting. We identified overexpression of Dock180 and Elmo1 in human melanoma compared to normal skin ex vivo. Inhibition of Dock180 or Elmo1 following siRNA treatment in melanoma cells reduced cell viability and increased apoptosis as supported by increased proportion of cells with Annexin V-PE(+) staining and sub-G0/G1 peak in cell cycle analysis. Moreover, inhibition of Dock180 or Elmo1 regulated apoptosis-related proteins, showing downregulaton of Bcl-2, caspase-3, and PARP and upregulation of Bax, PUMA, cleaved caspase3, and cleaved PARP. Furthermore, knockdown of Dock180 and Elmo1 in melanoma cells reduced cell migration and changed cellular signaling pathways including ERK and AKT. Vemurafenib treatment decreased cell viability in a concentration-dependent manner, while transfection with Dock180- or Elmo1-specific siRNA in melanoma cells significantly reduced cell viability compared to non-transfected cells. Collectively, these findings suggest that both Dock180 and Elmo1 may be associated with cancer progression such as cell survival and migration in melanoma, and can be potential targets for treatment of melanoma.
Assessing Visualization in Robotic-Assisted Surgery: Demystifying a Misty Lens
Authors: Dae Jessica Y. , Ginjupalli Mahija , Rickmeyer Zachary , Uecker John M. , Laviana Aaron , Idelson Christopher R. 
Published: April 2022

Abstract
Background
Despite major technological advancements in robotic-assisted laparoscopic surgery (RAS), there remain shortcomings yet to be addressed. This study assesses the prevalence of suboptimal vision in minimally invasive RAS and corresponding factors regarding related surgical conditions.
Methods
45 minimally invasive robotic surgeries, performed using Da Vinci XI, were observed across three surgical subspecialties: general, urology, and OB/GYN. Lens occlusion events were monitored and defined as the presence of a visual distortion caused by debris deposition on the scope lens. Lens occlusions and cleanings, and “active instrumentation” were recorded. Descriptive statistics summarized duration-based variables, and one-factor ANOVA compared the presence of active instrumentation.
Results
Cases averaged 127 ± 76 minutes. Active instrumentation ANOVA during lens occlusions demonstrated significant variation between categories (F7, 256 = 11.63, P = 2.558e-13). Post-hoc Tukey HSD found electrocautery devices were active significantly more during occlusion events (37.9%) than other instruments. On average, lens cleaning occurred every 36.5 ± 39.8 minutes despite lens occlusion occurring every 24.5 ± 15.7 minutes. Of the operative time observed, 41.4% ± 28.1% was conducted with visual distortion. 1.16% ± 0.97% of time observed was spent cleaning.
Conclusions
Although only 1.16% of operative time was spent cleaning, surgeons experienced suboptimal conditions for nearly 35x the time it would take the clear lens, potentially indicating a tendency to avoid cleaning the lens to disrupt surgery. Future research may examine the impact of occluded visualization and lens cleaning on other aspects of surgery.
Does local government competition improve ecological welfare performance? Evidence from China
Authors: Lv Yanqin , Fan Tianzheng , Zhang Jinning , Zhao Bin 
Published: April 2022

Abstract
Improving the efficiency of converting natural resources into social benefits is an important issue for sustainable development in today's world, at the same time, competition among local governments in China has important implications for both economy development and environment damage. Based on this, this paper measures the ecological welfare performance (EWP) of 30 provinces in China from 2005 to 2019, and empirically analyzes the impact of local government competition on EWP by using the spatial Durbin and spatial quantile models. It was found that: EWP in China has a significant spatial correlation, and government competition has a facilitating effect on EWP in both local as well as neighboring regions. The spatial Durbin quantile regression results indicate that government competition does not have a significant effect on EWP at higher quantile levels. In addition, there is significant regional heterogeneity, Government competition in the eastern region can only promote the EWP in local province without spatial spillover. In contrast, government competition in the center inhibits EWP not only in local province, but also in neighboring provinces, and there is white-hot "cut-throat competition" between provinces, with a significant negative spatial spillover.
Recurrent culture-negative endocarditis and osteomyelitis caused by Q fever in Iran, a case report
Authors: Moradi Maryam , Kanafgorabi Mahsa Azadbakhsh , Larti Farnoosh , Ghiasvand Fereshteh , Esmaeili Saber , Ghaderkhani Sara 
Published: April 2022

Abstract
Background: Endocarditis is an infection of the endocardial layer of the heart known as fatal infection. Gold standard diagnosis of infectious endocarditis is blood culture, which in some cases can be negative. In blood culture-negative endocarditis, the early diagnosis, and treatment are much harder, which can increase morbidities and also mortality. Case presentation: In this case report we are presenting a patient with intermittent fever for three months with a history of aortic and pulmonary valve replacement and also recurrent blood culture-negative endocarditis. According to the pandemic situation, we checked the Covid-19 PCR and also performed a chest computed tomography (CT) -scan, which both were negative and did not represent any pathologic conditions. Other examinations such as transesophageal echocardiography (TEE) and blood cultures were all normal and the only abnormal finding we had was a positron emission tomography (PET) - CT scan with endocarditis and sternum osteomyelitis evidence. Conclusion: After several evaluations based on endemic epidemiology, the Real-time PCR and IFA (IgG phase I; 1:16384, IgG phase II; 1:16384) were positive for Q fever and the patient responded to the proper doxycycline and hydroxychloroquine treatment.