bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Silico Identification of Potential Inhibitors of the SARS-CoV-2 Nucleocapsid Through Molecular Docking-Based Drug Repurposing
Authors: Afreen Rukhsar , Iqbal Saleem , Shah Ab Rauf , Afreen Heena , Vodwal Lata , Shkir Mohd. 
Published: May 2022

AbstractSARS-CoV-2 is the virus responsible for the COVID-19 pandemic, and its effects on people worldwide continue to grow. Protein-targeted therapeutics are currently unavailable for this virus. As with other coronaviruses, the nucleocapsid (N) protein is the most conserved RNA-binding structural protein of SARS-CoV-2. The N protein is an appealing target because of its functional role in viral transcription and replication. Therefore, molecular docking method for structure-based drug design was used to investigate the binding energy and binding modes of various anti-N inhibitors in depth. The inhibitors selected were originally developed to target stress granules and other molecules involved in RNA biology, and were either FDA-approved or in the process of clinical trials for COVID-19. We aimed at targeting the N-terminal RNA binding domain (NTD) for molecular docking-based screening, on the basis of the first resolved crystal structure of SARS-CoV-2 N protein (PDB ID: 6M3M) and C-terminal domain (CTD) dimerization of the nucleocapsid phosphoprotein of SARS-COV-2 (PDB ID: 6WJI). Silmitasertib, nintedanib, ternatin, luteolin, and fedratinib were found to interact with RNA binding sites and to form a predicted protein interface with high binding energy. Similarly, silmitasertib, sirolimus-rapamycin, dovitinib, nintedanib, and fedratinib were found to interact with the SARS-CoV-2 N protein at its CTD dimerization sites, according to previous studies. In addition, we investigated an information gap regarding the relationships among the energetic landscape and stability and drug binding of the SARS-CoV-2 N NTD and CTD. Our in silico results clearly indicated that several tested drugs as potent putative inhibitors for COVID-19 therapeutics, thus indicating that they should be further validated as treatments to slow the spread of SARS-CoV-2.
Transatlantic Socialist Feminisms in the Cold War World
Authors: Donert Celia 
Published: May 2022

AbstractThis review essay engages with Dorothy Sue Cobble's For The Many: American Feminists and the Global Fight for Democratic Equality from the perspective of European histories of socialist feminism during the Cold War. The essay suggests three themes that might lead to further discussion. These concern first of all the role of left-Catholic as well as Social Democratic women within the networks that Cobble describes in For the Many; second, the influence of nationalist or other exclusionary discourses on debates about the rights of immigrant workers, and third, the role of social democratic actors in shaping debates about working women's rights in other international organizations - particularly regional organizations such as the EEC/EU. The essay concludes that For the Many is a major contribution to our understanding of transatlantic socialist feminisms in the Cold War world.
Beyond skills and knowledge: the role of self-efficacy and peer networks in building capacity for species conservation planning
Authors: Bruyere Brett L. , Copsey Jamieson , Walker Sarah E. 
Published: May 2022

AbstractBiodiversity loss is one of the greatest global challenges and requires substantial investment in building the capacity of conservation professionals to design and implement robust conservation plans. In this study, we surveyed 155 past participants of training in facilitating species conservation planning processes given by the Conservation Planning Specialist Group of the IUCN Species Survival Commission. Based on a recently developed theory of change for the training, we examined how and to what extent the training contributed to the desired outcome of increasing trainees’ capacity for leading the design and facilitation of species conservation planning processes. Our results indicate that recall of training content, self-efficacy (an individual's belief they can complete a specific task or behaviour successfully) and peer network participation had significant impacts on the outcome of applying training content in the workplace. Furthermore, our results suggest that self-efficacy played a highly influential role in trainees' participation in species conservation planning post-training. The implications of this research point to designing conservation training that considers not only the skills and knowledge to be gained by learners but also the strategies that enhance trainees' self-efficacy in applying new skills and knowledge and in establishing peer networks to support trainees in turning training objectives into realities.
Effect of design parameters on strong and immobilizing grasps with an underactuated robotic hand
Authors: Hota Roshan Kumar , Kumar Cheruvu Siva 
Published: May 2022

AbstractThis paper presents a study on the effect of design parameters of an underactuated hand on its grasp performance. Three kinds of grasp performance characteristics are considered: grasp range, grasp strength, and immobilizing grasp range. Grasp strength is defined as the stiffness of the grasp. Immobilizing grasps are those in which the object cannot be moved for a force up to a certain threshold. In general, underactuated hands cannot produce immobilizing grasps. However, we show that immobilizing grasps can be created by including joint limits in the hand design. We consider the effect of two design parameters on the grasp performance: torque ratio and finger-base distance. Results show that an optimal finger-base distance and torque ratio exists that maximizes the grasp range and grasp strength. Also, the immobilizing grasp range is increased by decreasing the finger-base distance and increasing the torque ratio and joint limits.
In Pursuit of Racial Equality: Identifying the Determinants of Support for the Black Lives Matter Movement with a Systematic Review and Multiple Meta-Analyses
Authors: Azevedo Flavio , Marques Tamara , Micheli Leticia 
Published: May 2022

The Black Lives Matter (BLM) movement arose to put a much-needed spotlight on police brutality and systemic racism. In two comprehensive studies, we sought to investigate the determinants of support for the BLM movement. First, in a systematic review 1,588 records were identified and findings from twenty-four studies (Npooled=27,691) were narratively synthesized along five categories relating to demographics, race, partisanship and ideology, discrimination and prejudice, and psychology. Second, we exhaustively examined the determinants of BLM support across thirteen probability-based nationally representative datasets (Npooled=31,779), finding thirty-seven common predictors for which individual meta-analyses were conducted to estimate the strength and robustness of their associations. Our results suggest a near perfect match between BLM opposition and positive attitudes towards political actors and institutions rooted in systemic racism in the United States. The present work contributes to a broad categorization of correlates of support for BLM across social, psychological, and political domains.
Radiation Properties Improvement of 1 × 4 Array of Wideband Printed Antenna Supported by AMC Surface for Vehicular MIMO Systems
Authors: Malekpoor Hossein , Shahraki Mojtaba 
Published: May 2022

AbstractA 1 × 4 array of printed dipole antenna (PDA) with a broadband rhomboid artificial magnetic conductor (AMC) is introduced. A suggested PDA using a pair of microstrip dipoles with unequal arms excited by a T‐shaped microstrip feedline expands the bandwidth in the measured range of 5.27–6.74 GHz. Then, the suggested 3 × 3 AMC reflector is inserted into the PDA to exhibit −10 dB measured impedance bandwidth from 4.20 to 7.11 GHz for WLAN and WiMAX applications. The suggested PDA with AMC compared to the PDA without AMC exhibits a size reduction of 46.72%, enhanced gain up to 8.4 dBi, and excellent impedance matching with unidirectional radiation patterns. The novel AMC unit cell operates at 6.20 GHz with an AMC bandwidth of 5.09–7.21 GHz. Also, by loading a 3 × 8 AMC periodic array on a FR4 substrate with 3 mm thickness underneath the four‐element array of PDA with a gap distance of 3 mm, a low‐profile wideband structure with enhanced radiation properties is achieved. The measured S‐parameters show the broad bandwidth from 4.26 to 7.28 GHz with enhanced gains and efficiencies of all elements. Besides, the suitable isolation between the array elements of more than 30 dB for multiple‐input multiple‐output (MIMO) systems is achieved by applying various polarized directions of PDAs and loading the 3 × 8 periodic AMC reflector. Finally, the proposed MIMO design introduces remarkable advantages of good size reduction and bandwidth enhancement, low‐profile antenna with high stable gain, high efficiency and large isolation, and unidirectional radiation patterns with reduced backside direction.
The Chemistry of Mercury in the Stratosphere
Authors: Saiz‐Lopez Alfonso , Acuña A. Ulises , Mahajan Anoop S. , Dávalos Juan Z. , Feng Wuhu , Roca‐Sanjuán Daniel , Carmona‐García Javier , Cuevas Carlos A. , Kinnison Douglas E. , Gómez Martín Juan Carlos , Francisco Joseph S. , Plane John M. C. 
Published: May 2022

AbstractMercury, a global contaminant, enters the stratosphere through convective uplift, but its chemical cycling in the stratosphere is unknown. We report the first model of stratospheric mercury chemistry based on a novel photosensitized oxidation mechanism. We find two very distinct Hg chemical regimes in the stratosphere: in the upper stratosphere, above the ozone maximum concentration, Hg0 oxidation is initiated by photosensitized reactions, followed by second‐step chlorine chemistry. In the lower stratosphere, ground‐state Hg0 is oxidized by thermal reactions at much slower rates. This dichotomy arises due to the coincidence of the mercury absorption at 253.7 nm with the ozone Hartley band maximum at 254 nm. We also find that stratospheric Hg oxidation, controlled by chlorine and hydroxyl radicals, is much faster than previously assumed, but moderated by efficient photo‐reduction of mercury compounds. Mercury lifetime shows a steep increase from hours in the upper‐middle stratosphere to years in the lower stratosphere.
Bursty Bulk Flow Turbulence as a Source of Energetic Particles to the Outer Radiation Belt
Authors: Ergun R. E. , Usanova M. E. , Turner D. L. , Stawarz J. E. 
Published: May 2022

AbstractWe report observations of a Bursty Bulk Flow (BBF) penetrating close to the outer edge of the radiation belt. The turbulent BBF braking region is characterized by ion velocity fluctuations, magnetic field (B) variations, and intense electric fields (E). In this event, energetic (>100 keV) electron and ion fluxes are appreciably enhanced. Importantly, fluctuations in energetic electrons and ions suggest local energization. Using correlation distances and other observed characteristics of turbulent E, test‐particle simulations support local energization by E that favors higher‐energy electrons and leads to an enhanced energetic shoulder and tail in the electron distributions. The energetic shoulder and tail could be amplified to MeV energies by adiabatic transport into the radiation belt where |B| is higher. This analysis suggests that turbulence generated by BBFs can, in part, supply energetic particles to the outer radiation belt and that turbulence can be a significant contributor to particle acceleration.
A Lower Mantle Slab Below the East Asia Margin Constrained by Seismic Waveform Complexity
Authors: Yu Jing , Sun Daoyuan 
Published: May 2022

AbstractThe precise location, morphology, and physical properties of the slab in the lower mantle provide critical information for deciphering the subduction history. Here, we study the waveform complexity of the data recorded by the dense China National Seismograph Network and delineate a lower mantle slab structure below the East Asia margin. For the events that occurred in the Solomon Island area, we observe fast and strongly multipathed S waveforms at the stations near the Sichuan Basin. Furthermore, the observed multipathing patterns vary among different events, which provide robust constraints for the depth of this lower mantle slab. Through detailed waveform modeling, we confirm that a southeastern‐dipping low‐mantle slab exists at a depth of 1,000 km below the East Asia margin with a shear wave velocity perturbation of 2.5%, a length of 1,000 km, and a thickness of 200 km. The spatial correlation between the imaged lower mantle slab and the plate reconstruction model suggests that this slab may be a fragment of the Izanagi Plate from the late Cretaceous subducted beneath the proto South China Sea. However, this southeastern‐dipping structure may invoke the hypothesis of intraoceanic subduction within the Izanagi Plate since the late Cretaceous, which might exist further south of the western Pacific.
A User Guide for Choosing Planktic Foraminiferal Mg/Ca‐Temperature Calibrations
Authors: Rosenthal Yair , Bova Samantha , Zhou Xiaoli 
Published: May 2022

AbstractAlthough foraminiferal magnesium/calcium (Mg/Ca) measurements are now widely used for reconstructing sea surface temperature (SST), there is uncertainty about the fidelity of different calibrations of this proxy. Whereas single‐variable calibrations suggest temperature sensitivity of about 9–10% per °C, multivariable calibrations suggest ∼6% per °C, with additional dependence on salinity and pH. Here, we apply five calibrations to six published Mg/Ca records of Globigerinoides ruber, a planktic foraminifer typically used for reconstructing low latitude SST during the Pleistocene. Reconstructed SST by the different calibrations, spanning the past 250,000 years, can be offset by a few degrees, possibly reflecting variable dissolution or hydrographic effects. However, for 4 out of 5 calibrations, the reconstructed temperature anomalies yield estimates that are consistent within the calibrations' uncertainty (<±1 °C), despite the fundamental differences in temperature sensitivity among the equations. We further propose a new seawater Mg/Ca record for the late Neogene and show that the same consistency holds for longer time scales (∼4 My) independently of the choice of the calibration or which seawater Mg/Ca record is used. These comparisons attest to the robustness of the calibrations despite all the confounding nonthermal effects, and offer an empirical basis for researchers and reviewers to judge the records without any prejudice about which calibration is the “best” and evaluate their uncertainties.
Hepatocyte Bcl-3 protects from death-receptor mediated apoptosis and subsequent acute liver failure
Authors: Gehrke Nadine , Wörns Marcus A. , Mann Amrit , Hövelmeyer Nadine , Waisman Ari , Straub Beate K. , Galle Peter R. , Schattenberg Jörn M. 
Published: May 2022

AbstractAcute liver failure (ALF) is a rare entity but exhibits a high mortality. The mechanisms underlying ALF are not completely understood. The present study explored the role of the hepatic B cell leukemia-3 (Bcl-3), a transcriptional regulator of nuclear factor-kappa B (NF-κB), in two independent models of ALF. We employed a recently developed transgenic mouse model in a C57BL6/J background comparing wild-type (WT) and transgenic littermates with hepatocyte-specific overexpression of Bcl-3 (Bcl-3Hep) in the ALF model of d-galactosamine (d-GalN) and lipopolysaccharide (LPS). Additionally, the apoptosis-inducing CD95 (FAS/APO-1)-ligand was explored. Bcl-3Hep mice exhibited a significant protection from ALF with decreased serum transaminases, decreased activation of the apoptotic caspases 8, 9, and 3, lower rates of oxidative stress, B-cell lymphoma 2 like 1 (BCL2L1/BCL-XL) degradation and accompanying mitochondrial cytochrome c release, and ultimately a decreased mortality rate from d-GalN/LPS compared to WT mice. d-GalN/LPS treatment resulted in a marked inflammatory cytokine release and stimulated the activation of signal transducer and activator of transcription (STAT) 3, c-Jun N-terminal kinases (JNK) and extracellular signal-regulated kinase (ERK) signaling comparably in the hepatic compartment of Bcl-3Hep and WT mice. However, in contrast to the WT, Bcl-3Hep mice showed a diminished rate of IkappaB kinase-beta (IKK-β) degradation, persistent receptor interacting protein kinase (RIPK) 1 function and thus prolonged cytoprotective nuclear factor-kappa B (NF-κB) p65 signaling through increased p65 stability and enhanced transcription. Likewise, Bcl-3 overexpression in hepatocytes protected from ALF with massive hepatocyte apoptosis induced by the anti-FAS antibody Jo2. The protection was also linked to IKK-β stabilization. Overall, our study showed that Bcl-3 rendered hepatocytes more resistant to hepatotoxicity induced by d-GalN/LPS and FAS-ligand. Therefore, Bcl-3 appears to be a critical regulator of the dynamics in ALF through IKK-β.
Compound heterozygous variants in MAN2B2 identified in a Chinese child with congenital disorders of glycosylation
Authors: Tian Qi , Shu Li , Shu Chuqiang , Xi Hui , Ma Na , Mao Xiao , Wang Hua 
Published: May 2022

AbstractCongenital disorders of glycosylation (CDG) is a group inherited disorders. It is characterized by multi-organ dysfunction with significant morbidity and mortality. MAN2B2-CDG caused by pathogenic variants in the MAN2B2 gene was a rare CDG. To date, only one case of MAN2B2-CDG was reported. The representative clinical features were immune deficiency, dysmorphic facial features, coagulopathy, and severe developmental delay. More cases are needed to support the pathogenesis of MAN2B2 variation and elucidate its clinical heterogeneity. In this study, we described the clinical presentations of a CDG proband with compound heterozygous variants in MAN2B2. Serum N-glycan profiling was measured by MALDI coupled to time-of-flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS analysis of patient serum showed disorders of N-linked glycosylation, including increased N-glycans and elevated Man5/Man6 and Man5/Man9 value. Our proband presented severe developmental delay, dysmorphic facial features as in the previous case. But our case presented new features, including cleft palate and hypospadias with no immune deficiency. Our data expands both the molecular and clinical phenotypes of MAN2B2-CDG and highlights the importance of the role of MAN2B2 gene in CDG.
Highly stable and tunable peptoid/hemin enzymatic mimetics with natural peroxidase-like activities
Authors: Jian Tengyue , Zhou Yicheng , Wang Peipei , Yang Wenchao , Mu Peng , Zhang Xin , Zhang Xiao , Chen Chun-Long 
Published: May 2022

AbstractDeveloping tunable and stable peroxidase mimetics with high catalytic efficiency provides a promising opportunity to improve and expand enzymatic catalysis in lignin depolymerization. A class of peptoid-based peroxidase mimetics with tunable catalytic activity and high stability is developed by constructing peptoids and hemins into self-assembled crystalline nanomaterials. By varying peptoid side chain chemistry to tailor the microenvironment of active sites, these self-assembled peptoid/hemin nanomaterials (Pep/hemin) exhibit highly modulable catalytic activities toward two lignin model substrates 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 3,3’,5,5’-tetramethylbenzidine. Among them, a Pep/hemin complex containing the pyridyl side chain showed the best catalytic efficiency (Vmax/Km = 5.81 × 10−3 s−1). These Pep/hemin catalysts are highly stable; kinetics studies suggest that they follow a peroxidase-like mechanism. Moreover, they exhibit a high efficacy on depolymerization of a biorefinery lignin. Because Pep/hemin catalysts are highly robust and tunable, we expect that they offer tremendous opportunities for lignin valorization to high value products.
Dynamic arrest and aging of biomolecular condensates are modulated by low-complexity domains, RNA and biochemical activity
Authors: Linsenmeier Miriam , Hondele Maria , Grigolato Fulvio , Secchi Eleonora , Weis Karsten , Arosio Paolo 
Published: May 2022

AbstractBiomolecular condensates require suitable control of material properties for their function. Here we apply Differential Dynamic Microscopy (DDM) to probe the material properties of an in vitro model of processing bodies consisting of out-of-equilibrium condensates formed by the DEAD-box ATPase Dhh1 in the presence of ATP and RNA. By applying this single-droplet technique we show that condensates within the same population exhibit a distribution of material properties, which are regulated on several levels. Removal of the low-complexity domains (LCDs) of the protein decreases the fluidity of the condensates. Structured RNA leads to a larger fraction of dynamically arrested condensates with respect to unstructured polyuridylic acid (polyU). Promotion of the enzymatic ATPase activity of Dhh1 reduces aging of the condensates and the formation of arrested structures, indicating that biochemical activity and material turnover can maintain fluid-like properties over time.
Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells
Authors: Zhou Qiyin , Lin Wenyu , Wang Chaoqun , Sun Fei , Ju Siwei , Chen Qian , Wang Yi , Chen Yongxia , Li Haomin , Wang Linbo , Hu Zeping , Jin Hongchuan , Wang Xian , Sun Yi 
Published: May 2022

AbstractAbnormal neddylation activation is frequently observed in human cancers and neddylation inhibition has been proposed as a therapy for cancer. Here, we report that MLN4924, a small-molecule inhibitor of neddylation activating enzyme, increases glutamine uptake in breast cancer cells by causing accumulation of glutamine transporter ASCT2/SLC1A5, via inactivation of CRL3-SPOP E3 ligase. We show the E3 ligase SPOP promotes ASCT2 ubiquitylation, whereas SPOP itself is auto-ubiquitylated upon glutamine deprivation. Thus, SPOP and ASCT2 inversely regulate glutamine uptake and metabolism. SPOP knockdown increases ASCT2 levels to promote growth which is rescued by ASCT2 knockdown. Adding ASCT2 inhibitor V-9302 enhances MLN4924 suppression of tumor growth. In human breast cancer specimens, SPOP and ASCT2 levels are inversely correlated, whereas lower SPOP with higher ASCT2 predicts a worse patient survival. Collectively, our study links neddylation to glutamine metabolism via the SPOP-ASCT2 axis and provides a rational drug combination for enhanced cancer therapy.
Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling
Authors: Moriyama Hidenori , Endo Jin , Kataoka Masaharu , Shimanaka Yuta , Kono Nozomu , Sugiura Yuki , Goto Shinichi , Kitakata Hiroki , Hiraide Takahiro , Yoshida Naohiro , Isobe Sarasa , Yamamoto Tsunehisa , Shirakawa Kohsuke , Anzai Atsushi , Katsumata Yoshinori , Suematsu Makoto , Kosaki Kenjiro , Fukuda Keiichi , Arai Hiroyuki , Sano Motoaki 
Published: May 2022

AbstractPulmonary hypertension is a fatal rare disease that causes right heart failure by elevated pulmonary arterial resistance. There is an unmet medical need for the development of therapeutics focusing on the pulmonary vascular remodeling. Bioactive lipids produced by perivascular inflammatory cells might modulate the vascular remodeling. Here, we show that ω-3 fatty acid-derived epoxides (ω-3 epoxides) released from mast cells by PAF-AH2, an oxidized phospholipid-selective phospholipase A2, negatively regulate pulmonary hypertension. Genetic deletion of Pafah2 in mice accelerate vascular remodeling, resulting in exacerbation of hypoxic pulmonary hypertension. Treatment with ω-3 epoxides suppresses the lung fibroblast activation by inhibiting TGF-β signaling. In vivo ω-3 epoxides supplementation attenuates the progression of pulmonary hypertension in several animal models. Furthermore, whole-exome sequencing for patients with pulmonary arterial hypertension identifies two candidate pathogenic variants of Pafah2. Our findings support that the PAF-AH2-ω-3 epoxide production axis could be a promising therapeutic target for pulmonary hypertension.