bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tanshinone IIA attenuates the stemness of breast cancer cells via targeting the miR-125b/STARD13 axis
Authors: Li Xiaoman , Jia Qi , Zhou Yinyin , Jiang Xuan , Song Li , Wu Yuanyuan , Wang Aiyun , Chen Wenxing , Wang Shijun , Lu Yin 
Published: January 2022

AbstractBackgroundTanshinone II A is an effective component extracted from Salvia miltiorrhiza and the roles of Tanshinone IIA in regulating the stemness of tumor cells remain unclear. This work aims to explore the roles and underlying mechanisms of Tanshinone IIA in breast cancer stemness.MethodsIn vitro mammary spheroid formation, flow cytometry assay on CD24−/CD44+sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells. MiRNAs-based transcriptome sequencing and data analysis, online dataset analysis, luciferase reporter assay combined with rescuing experiments were constructed to explore the underlying mechanisms.ResultsTanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation, decreasing the CD24−/CD44+sub-population in a concentration-dependent manner and reducing the tumor-initiating ability of breast cancer cells. Additionally, Tanshinone IIA enhanced adriamycin sensitivity and attenuated adriamycin resistance of breast cancer cells. Combined with miRNAs-based transcriptome sequencing assay, it was found that Tanshinone IIA downregulated miR-125b level and upregulated its target gene STARD13 (StAR-related lipid transfer protein 13) level, thus inactivating the miR-125b/STARD13 axis, which had been previously confirmed to promote breast cancer progression. Notably, miR-125b overexpression enhanced the stemness of breast cancer cells, and miR-125b overexpression or STARD13 knockdown impaired the inhibitory effects of Tanshinone IIA on the stemness of breast cancer cells.ConclusionsTanshinone IIA could attenuate the stemness of breast cancer cells via targeting the miR-125b/STARD13 axis.
SLC3A2 inhibits ferroptosis in laryngeal carcinoma via mTOR pathway
Authors: Wu Fangxing , Xiong Gaoyun , Chen Zejun , Lei Chenyang , Liu Qianqian , Bai Yundan 
Published: January 2022

Abstract
Objective
This study aimed to explore the mRNA and protein expression of SLC3A2 in laryngeal carcinoma cells and tissues, and functional regulatory mechanism of SLC3A2 in cell ferroptosis of laryngeal carcinoma.

Methods
We chose the key gene-SLC3A2 of DEGs from TCGA by bioinformatics analysis, and then we constructed stable knockdown of SLC3A2 in laryngeal carcinoma cells. MTT assay and clonogenic assay were used to determine cell viability and cell growth, respectively. The mRNA and protein expression were determined by RT-qPCR and western blotting, respectively. Xenograft tumor model was used to determine the role of SLC3A2 in tumor growth.

Results
The results of limma analysis recovered that 92 genes were involved in both upregulated DEGs and high risk of poor prognosis, whereas 36 genes were involved in both downregulated DEGs and low risk of poor prognosis. Pathway enrichment analysis indicated that mTOR signaling pathway and ferroptosis exerted a role in regulating these intersection genes. Moreover, SLC3A2 is a key gene in ferroptosis in laryngeal carcinoma. SLC3A2 is highly expressed in laryngeal carcinoma tissues and cells. Patients with high SLC3A2 expression exerted poor survival. SLC3A2 deficiency inhibited cell proliferation and foci formation. Furthermore, knockdown of SLC3A2 expression induced the efficacy of ferroptosis and suppressed ferroptosis related proteins expression. Mechanically, SLC3A2 deficiency facilitated ferroptosis through upregulating the expression of mTOR and P70S6K, whereas inhibited p-mTOR and p-P70S6K expression in laryngeal carcinoma cells. SLC3A2 deficiency inhibited tumorigenesis in nude mice.

Conclusion
Our study suggests that SLC3A2 negatively regulates ferroptosis through mTOR pathway in laryngeal carcinoma.
Predicting singleton antepartum stillbirth by the demographic Fetal Medicine Foundation Risk Calculator—A retrospective case-control study
Authors: Muin Dana A. , Windsperger Karin , Attia Nadia , Kiss Herbert 
Published: January 2022

Objective
To assess the risk of singleton intrauterine fetal death (IUFD) in women by the demographic setting of the online Fetal Medicine Foundation (FMF) Stillbirth Risk Calculator.

Methods
Retrospective single-centre case-control study involving 144 women having suffered IUFD and 247 women after delivery of a live-born singleton. Nonparametric receiver operating characteristics (ROC) analyses were performed to predict the prognostic power of the FMF Stillbirth risk score and to generate a cut-off value to discriminate best between the event of IUFD versus live birth.

Results
Women in the IUFD cohort born a significantly higher overall risk with a median FMF risk score of 0.45% (IQR 0.23–0.99) compared to controls [0.23% (IQR 0.21–0.29); p<0.001]. Demographic factors contributing to an increased risk of IUFD in our cohort were maternal obesity (p = 0.002), smoking (p<0.001), chronic hypertension (p = 0.015), antiphospholipid syndrome (p = 0.017), type 2 diabetes (p<0.001), and insulin requirement (p<0.001). ROC analyses showed an area under the curve (AUC) of 0.72 (95% CI 0.67–0.78; p<0.001) for predicting overall IUFD and an AUC of 0.72 (95% CI 0.64–0.80; p<0.001), respectively, for predicting IUFD excluding congenital malformations. The FMF risk score at a cut-off of 0.34% (OR 6.22; 95% CI 3.91–9.89; p<0.001) yielded an 82% specificity and 58% sensitivity in predicting IUFD with a positive and negative predictive value of 0.94% and 99.84%, respectively.

Conclusion
The FMF Stillbirth Risk Calculator based upon maternal demographic and obstetric characteristics only may help identify women at low risk of antepartum stillbirth.
Lockdown como medida de intervenção para mitigar a propagação da COVID-19: um estudo de modelagem
Authors: Santana-Santos Eduesley , Gois Aédson Nascimento , Laureano Estêvão Esmi , Santos David da Silva , Santos Luiz Fernando Souza , Sánchez Daniel Eduardo , Vieira Rita de Cássia Almeida , Oliveira Jussiely Cunha 
Published: January 2022

Introdução: Este trabalho visa desenvolver um modelo biomatemático de transmissão da COVID-19, no estado de Sergipe, Brasil, a fim de estimar a distribuição dos casos ao longo do tempo e projetar o impacto na propagação do surto epidêmico devido às intervenções e medidas de controle sobre a população local. Métodos: Estudo de modelagem matemática epidemiológica, realizado para analisar a dinâmica dos casos acumulados de COVID-19, que utilizou um modelo de crescimento logístico que adiciona um termo de retirada de indivíduos como medida de controle. Foram simulados três possíveis cenários de propagação da COVID-19 baseados em três diferentes taxas de retirada de indivíduos. Cada uma das taxas é ajustada com dados reais sobre número de infetados e as medidas de controle sobre a população. Resultados: A medida extrema de isolamento total, ou lockdown, seria o melhor cenário, apresentando menor incidência de infectados, quando comparado às demais medidas. O número de infectados cresceria vagarosamente ao longo dos meses e o número de indivíduos sintomáticos nesse cenário seria de 40.265 casos. Percebeu-se que o Estado de Sergipe ainda encontra-se na fase inicial da doença, em quaisquer dos cenários. Foi possível observar que o pico dos casos e o equilíbrio, no cenário atual de isolamento social, se darão quando atingir a nova capacidade suporte, ao final de agosto em aproximadamente 1.171.353 indivíduos infectados. Conclusão: Percebeu-se que o lockdown é a intervenção com maior capacidade de mitigação da propagação do vírus pela população.
KAJIAN PROBLEMATIKA DAN STANDARISASI ASISTEN LABORATORIUM DI PERGURUAN TINGGI
Authors: Hikmah Fatimah Hikmah , Fahrudin Dimas , Setyowati Emy Shofiah 
Published: January 2022

<p>Perbedaan manajemen asisten dosen laboratorium pada berbagai jensi laboratorium disebabkan oleh adanya perbedaan kebijakan setiap kepala laboratorium. Untuk itu, dilakukan kajian mengenai problematika dan standarisasi asisten laboratorium di perguruan tinggi, utamanya prodi pendidikan sains pada jenjang sarjana. Penelitian dilakukan menggunakan pendekatan deskriptif kualitatif untuk menganalisis input, proses dan output dalam Perguruan Tinggi. Data kualitatif diperoleh melalui kegiatan wawancara kepada asisten dosen di beberapa perguruan tinggi seperti Universitas Negeri Malang, Universitas Negeri Jember dan Universitas Sebelas Maret. Wawancara berisi pertanyaan terkait proses perekrutan, kegiatan dalam laboratorium saat praktikum dan setelah praktikum serta reward yang didapatkan setelah menjadi asisten dosen laboratorium. Hasil yang diperoleh bahwa manajemen laboratorium mengenai asisten laboratorium dilapangan menemui beberapa permasalahan seperti tidak ada standarisasi terkait syarat/kualifikasi perekrutan asisten laboratorium, tidak ada honorarium, dan mahasiswa tidak mendapat sertifikasi setelah selesai melaksanakan tugas. Selain itu, belum terdapat standarisasi manajemen asisten dosen laboratorium dalam skala nasional.</p>
Systematic Review: The Covid-19 Pandemic Effects on Maternal Anxiety During Pregnancy
Authors: Argaheni Niken Bayu 
Published: January 2022

<p><strong>Background:</strong> Anxiety is a common response to any stressful situation. Pregnant women are more likely to catch viral illnesses, and the Coronavirus can cause stress and anxiety in pregnant women in different parts of the world. The purpose of this study was to determine the effect of the Covid-19 pandemic on maternal anxiety during pregnancy.</p><p><strong>Method:</strong> Systematic review using the database: Google Scholar. The search results that meet the criteria are then analyzed for articles.</p><p><strong>Result:</strong> The saturation of mass media and social media with news related to COVID-19 and the dissemination of information in it provides a climate of fear, uncertainty and anxiety, about the present and the future. This will be magnified for pregnant women today who, even without a pandemic, generally pregnant women experience feelings of uncertainty and anxiety related to childbirth, childbirth, the presence of a baby and the care of a newborn.</p><p><strong>Conclusion:</strong> Pregnant women have a greater psychological impact as well as higher anxiety during the COVID-19 pandemic. Identification of pregnant women who are at high risk of anxiety that occurs during the Covid-19 pandemic is important so that health workers can suggest early psychological intervention and prevent some complications related to stress in pregnancy.</p><p> </p>
The Impact of Mental Health on The Immune System During the Covid-19 Pandemic
Authors: Dharmawan Caroline , Argaheni Niken Bayu 
Published: January 2022

<p><strong><em>Background</em></strong><em>: The outbreak of Covid-19 virus in various countries has caused a lot of very significant changes. This Covid-19 outbreak has had profound impact on various aspects of life, from an economic, political, social perspective, and especially in terms of public health, especially in Indonesia. The symptoms caused by the Covid-19 virus are not only focused on physical health, but also affect psychological health. Goal: To prove that mental health affects immunity system.</em></p><p><em></em><strong><em>Methods:</em></strong><em> Systematic review.</em></p><p><strong><em>Result:</em></strong><em> The lower positive attitude, lack of social support, poor strategies in reducing the spread of virus, working with Covid-19 infected patient, causes high levels of stress. </em></p><p><strong><em>Conclusion:</em></strong><em> Prolonged stress and anxiety is decreasing body’s immune respons, thus making the body susceptible to disease. So that in overcoming this pandemic, beside focused on physical health, it is necessary to consider mental and psychosocial health</em><em> </em></p><p><strong>K</strong><strong>eywords</strong><strong>: </strong><em>Mental Health, Covid-19, Immune system</em></p>
A Two-Stage Diabetic Foot Salvage Using Synthetic Bone Void Filler and Lesser Toe Fillet Flap
Authors: Lee Jong Min , Del Balso Christopher , Gupta Sanjay , Tay Sherilyn , Daniels Timothy R. , Halai Mansur 
Published: January 2022

Case:
A 54-year-old type 2 diabetic man with a diabetic forefoot ulcer and osteomyelitis successfully underwent a staged reconstruction to salvage his foot. An antibiotic-carrying bone void filler was used to decrease the infection burden after initial debridement. The fifth toe fillet flap was performed to cover the large soft-tissue defect. The patient returned to baseline ambulation at the 1-year follow-up with a well-incorporated flap.

Conclusion:
This case highlights the utility of combining an antibiotic-carrying bone void filler and a toe fillet flap in a two-stage approach to salvage complex diabetic foot ulcers.
Aortic Injury After Shoulder Reduction in a Patient with Multiple Rib Fractures
Authors: Lim Eic-Ju , Kim Se-Heon , Shon Hyun-Chul , Yang Jae-Young 
Published: January 2022

Case:
A 74-year-old woman presented with multiple rib fractures, hemothorax, spleen rupture, and right shoulder dislocation. Shoulder joint reduction was performed using the traction-countertraction method. An injury to the descending aorta, caused by the seventh rib, was confirmed.

Conclusion:
The authors encountered an aortic injury, a rare complication of shoulder joint reduction, in a patient with a posterior rib fracture. When managing patients with posterior fractures of the fourth to ninth ribs on the left side, the reduction method should be carefully selected depending on the status of the patient to avoid aortic injuries.
Highly aligned bacterial nanocellulose films obtained during natural biosynthesis
Authors: Murugarren Nerea , Roig-Sanchez Soledad , Anton-Sales Irene , Malandain Nanthilde , Xu Kai , Solano Eduardo , Reparaz Juan , Laromaine Anna 
Published: January 2022

Abstract
Bacterial nanocellulose (BNC) is usually produced as films of randomly-organized highly pure cellulose nanofibers and its properties such as high water-holding capacity and porosity, tunable morphology, mechanical strength, and biocompatibility make it a unique material. As a result, BNC has attracted interest in the paper and food industry, biotechnology, photonics and optoelectronics. Ordered structures found in nature, as the reed leaf, and the potential properties appearing upon aligning polymers fibers (piezoelectricity, conductivity, optoelectronics, to name a few) inspired us to develop a straightforward method to achieve highly aligned BNC films (Herman’s order S-parameter of 0.85) benefiting from the natural bacteria biosynthesis and extrusion of the cellulosic fibers. We locally confined the bacteria at the bottom of a tube containing liquid culture media and statically incubated them for a few days. Due to the oxygen requirements of the bacteria and the water insolubility of BNC, cellulose nanofibers were synthesized in a single direction without entanglement, which allowed obtaining band-chain aligned BNC in the liquid phase. The obtained film is highly oriented within the total volume of the film, as shown by polarization-resolved second-harmonic generation signal (P-SHG) and Small Angle X-ray Scattering (SAXS). Additionally, we proved an increase of hydrophilicity, suitable to tissue engineering scaffolds as human fibroblasts successfully adhered and grew on it. Finally, we evaluate the thermal conductivity by two independent approaches, i.e. using the well-known 3ω-method, as well as through a recently developed contactless thermoreflectance approach, confirming a thermal conductivity of 1.63 W/mK in the direction of the aligned fibers in contrast with 0.3 W/mK in the perpendicular direction. The 5-fold increase in thermal conductivity of BNC in the parallel direction forecasts the potential of BNC-based devices outperforming some other natural polymer and synthetic materials.
Homocysteine Level Related To Age Is Associated With Embryo Quality In IVF Women With Diminished Ovarian Reserve
Authors: Wang Haiyan , Han Aiqing , Wei Bowen , Cao Dan , Jiang Yangyu , Sun Lin , Zou Na , Tao Shiying , Xue Xiaoou , Shao Xiaoguang , Li Jian 
Published: January 2022

Abstract
Background: Diminished ovarian reserve (DOR) contributes significantly to female infertility. Embryo quality affects pregnancy outcome. However, the factors that influence pregnancy outcomes in DOR patients are not well established. In this study, we analyzed the medical records of infertile women with DOR who underwent ART to reveal the possible correlation between clinical characteristics and embryo quality. Methods: A retrospective cohort study was conducted using 3390 medical record data on IVF cycles from Dalian Municipal Women and Children’s Medical Center (Group) from January 2015 to December 2020. The data was divided into poor-quality embryos group and high-quality embryos group according to the embryo status. We summarized the demographic and clinical characteristics (age, infertility years, infertility type, AFC, and BMI), medical history, reproductive hormone parameters (AMH, FSH, LH, PRL, and E2), and carbohydrate and lipid metabolic indexes (CHO, TG, LP, LDL, HDL, FINS, Glucose, and Hcy). To further evaluate the Hcy levels in patients with different embryo quality, we performe subgroup analysis. In order to further evaluate the Hcy levels of patients with different embryo quality, we conducted a subgroup analysis, which was divided into three subgroups: Hcy ≥15, 15> Hcy >8 and Hcy ≤8. A total of 1023patients with DOR were included in this study.Results: Of these, 483 cases were with high-quality embryos (the number of high quality embryo, number≥1), and 540 cases with poor-quality embryos (no high quality embryo, number=0). Demographic characteristics of the two groups were similar. However, the level of serum Hcy was significantly higher in the poor-quality embryo group compared with the high-quality embryo group (P < 0.05). In addition, the levels of serum AMH and PRL were statistically different between the two groups. Patients with serum Hcy ≥ 15 μmol/L were at higher risk of having poor-quality embryos (82.1%) compared to patients with serum Hcy less than 8 μmol/L (43.9%) and at 8~15 μmol/L (48.1%) (c2 =15.859, P < 0.001).Conclusion: The presented study demonstrated that high serum Hcy level is linked to poor-quality embryos. Moreover, Hcy coupled with age might be a sensitive marker for embryo quality after IVF.
Wolman Disease Presenting With HLH Syndrome and a Novel LIPA Gene Variant: a Case-based Review
Authors: Ashari Kosar Asna , Azari-Yam Aileen , Shahrooei Mohammad , Ziaee Vahid 
Published: January 2022

Abstract
Introduction: Wolman Disease (WD) is a rare disease caused by the absence of functional liposomal acid lipase (LAL) due to mutations in LIPA gene. It presents with organomegaly, malabsorption and adrenal calcifications. The presentations can resemble Hemophagocytic lymphohistiocytosis (HLH), the life threatening hyper-inflammatory disorder.Method: We present a 4.5-month-old boy with fever, icterus and hepatosplenomegaly who was treated according to presumed HLH disease. There are some case reports in the literature presenting patients with WD primarily diagnosed as HLH, which we discussed in this review.Results: The genetic analysis revealed after his demise was compatible with WD, introducing a novel mutation in LIPA gene: exon 4: NM_001127605: c. G353A (p.G118D), which converts the Glycine amino acid to Aspartic acid.Conclusions: Considering the similarities in presentation of WD and HLH, the patient’s life can be saved if special attention is paid to presenting features of a patient with suspected HLH, i.e., symptoms, finding on physical exams, laboratory values and radiologic findings, and the proper treatment is urgently initiated. Reporting the novel mutations of WD can help geneticists interpret the results of their patients’ genetic studies appropriately, leading to correct diagnosis and treatment.
Design and Optimization of Surface Plasmonic Sensor with Tunable Optical Actuation Angle based on Microsystems Technology for Microfluidic Application
Authors: Mokhtarpour Reza , Ghavifekr Habib Badri 
Published: January 2022

Abstract
Surface Plasmon resonance-based sensors are one of the most accurate detectors of chemical and biochemical components in microfluidic structures performed based on changes in the refractive index of the medium adjacent to the sensor surface. Precise adjustment of the excitation angle and occupation volume of one kinds of such sensors are among their highlighted challenges that come from conventional light coupling technique. In this paper, a novel idea for designing this type of sensor in microsystem technology is devised, which is capable of adjusting the angle of optical excitation using the thermal-optical effect while it has the minimum occupied volume. This design is optimized and validated using finite element method, so that the sensor performance in exchange for changes in parameters such as Refractive Index, metal Thickness, etc. is examined and displayed in the form of tables and graphs. Simulation results reveal that the proposed model is able to tune optical excitation angle such accurate that the SPR phenomenon occurs at an angle of 21.21˚at the outer boundary of 42 nm thick of gold layer at its best.
The Treatment and Outcome of Superior Mesenteric Artery Embolism: a Hospital-based Survey
Authors: Ito Ryogo , Aoba Taro , Yoshihara Motoi , Hiramatsu Kazuhiro 
Published: January 2022

Abstract
Objectives: The treatment for superior mesenteric artery (SMA) embolism is controversial because of the low prevalence of this disease. This study aimed to examine the treatment options for SMA embolism.Methods: We retrospectively reviewed the clinical data of twenty patients with SMA embolisms at the Toyohashi Municipal Hospital from April 2010 to March 2020. Clinical characteristics, findings, treatment and outcomes were evaluated.Results: The overall median age of the patients was 80 years old. In 16 cases, the obstructed regions of the SMA were proximal to the ileocecal artery (ICA) bifurcation. Nine cases had pneumatosis intestinalis (PI). Two patients underwent surgery, twelve patients received endovascular intervention (EI) and four patients received heparin treatment. Five patients died in the hospital. A comparative study of the outcomes after EI showed that the nonsurvivor group had a significantly higher rate of PI than the survivor group (P = 0.046). The patients that had obstructions proximal to the ICA bifurcation and the presence of PI had a high mortality rate of 60% after EI.Conclusions: Determining if there is the presence of PI and finding the obstructed region of the SMA are useful for evaluating the appropriate treatment for SMA embolisms.
The intensity of the plasmon-exciton of three spherical metal nanoparticles on the semiconductor quantum dot having three external fields
Authors: Ali Hagar M. Ali , abd-elnabi somia , Osman Kariman 
Published: January 2022

Abstract
The influence of the plasmonic of three spherical metal nanoparticles (MNPs) on the semiconductor quantum dot (SQD) having three external fields, is analyzed. The density matrix equations are modified for the description of the optical properties of the SQD-MNPs nanosystem. We study theoretically the role of the plasmon-exciton dipole coupling in the SQD-MNPs nanosystem. We investigate the dependence of the plasmon-exciton dipole coupling of the SQD-MNPs nanosystem on the position of three spherical MNPs with respect to SQD as well as on the material parameters of the hybrid nanosystem. The direction and detunings of the three external fields play an important role on the characterization of the SQD-MNPs nanosystem.