bluuebunny/crossref_metadata_2025_split huggingface dataset:
Das Gesetzbuch als Botschaft? – für eine andere Gesetzgebungsgeschichte
Authors: Caroni Pio 
Published: August 2022

Abstract

The Code as Message? Call for a Different Legislative History. The contribution examines the history of codification from a new perspective. The focus is not on the perspective of the legislator but rather on the perspective of those subjected to the laws. For, in fact, it is them who decide on the fate of the codification. Thus, a painstaking – and often tedious – history of impact should complete, that is replace the hitherto common and less complex textual history. Only such a history of impact enables more differentiated statements about the fate and importance of the code, for which a few examples are given.
Lint‐O cooperates with L(3)mbt in target gene suppression to maintain homeostasis in fly ovary and brain
Authors: Yamamoto‐Matsuda Hitomi , Miyoshi Keita , Moritoh Mai , Yoshitane Hikari , Fukada Yoshitaka , Saito Kuniaki , Yamanaka Soichiro , Siomi Mikiko C 
Published: August 2022

AbstractLoss‐of‐function mutations in Drosophila lethal(3)malignant brain tumor [l(3)mbt] cause ectopic expression of germline genes and brain tumors. Loss of L(3)mbt function in ovarian somatic cells (OSCs) aberrantly activates germ‐specific piRNA amplification and leads to infertility. However, the underlying mechanism remains unclear. Here, ChIP‐seq for L(3)mbt in cultured OSCs and RNA‐seq before and after L(3)mbt depletion shows that L(3)mbt genomic binding is not necessarily linked to gene regulation and that L(3)mbt controls piRNA pathway genes in multiple ways. Lack of known L(3)mbt co‐repressors, such as Lint‐1, has little effect on the levels of piRNA amplifiers. Identification of L(3)mbt interactors in OSCs and subsequent analysis reveals CG2662 as a novel co‐regulator of L(3)mbt, termed “L(3)mbt interactor in OSCs” (Lint‐O). Most of the L(3)mbt‐bound piRNA amplifier genes are also bound by Lint‐O in a similar fashion. Loss of Lint‐O impacts the levels of piRNA amplifiers, similar to the lack of L(3)mbt. The lint‐O‐deficient flies exhibit female sterility and tumorous brains. Thus, L(3)mbt and its novel co‐suppressor Lint‐O cooperate in suppressing target genes to maintain homeostasis in the ovary and brain.
eEF2 in the prefrontal cortex promotes excitatory synaptic transmission and social novelty behavior
Authors: Ma Xuanyue , Li Liuren , Li Ziming , Huang Zhengyi , Yang Yaorong , Liu Peng , Guo Daji , Li Yueyao , Wu Tianying , Luo Ruixiang , Xu Junyu , Ye Wen‐Cai , Jiang Bin , Shi Lei 
Published: August 2022

AbstractRegulation of mRNA translation is essential for brain development and function. Translation elongation factor eEF2 acts as a molecular hub orchestrating various synaptic signals to protein synthesis control and participates in hippocampus‐dependent cognitive functions. However, whether eEF2 regulates other behaviors in different brain regions has been unknown. Here, we construct a line of Eef2 heterozygous (HET) mice, which show a reduction in eEF2 and protein synthesis mainly in excitatory neurons of the prefrontal cortex. The mice also show lower spine density, reduced excitability, and AMPAR‐mediated synaptic transmission in pyramidal neurons of the medial prefrontal cortex (mPFC). While HET mice exhibit normal learning and memory, they show defective social behavior and elevated anxiety. Knockdown of Eef2 in excitatory neurons of the mPFC specifically is sufficient to impair social novelty preference. Either chemogenetic activation of excitatory neurons in the mPFC or mPFC local infusion of the AMPAR potentiator PF‐4778574 corrects the social novelty deficit of HET mice. Collectively, we identify a novel role for eEF2 in promoting prefrontal AMPAR‐mediated synaptic transmission underlying social novelty behavior.
Epidemiology of Neonatal COVID-19 in the United States
Authors: Devin Joan , Marano Rachel , Mikhael Michel , Feaster William , Sanger Terence , Ehwerhemuepha Louis 
Published: August 2022

OBJECTIVES
Data on coronavirus disease 2019 (COVID-19) infections in neonates are limited. We aimed to identify and describe the incidence, presentation, and clinical outcomes of neonatal COVID-19.

METHODS
Over 1 million neonatal encounters at 109 United States health systems, from March 2020 to February 2021, were extracted from the Cerner Real World Database. COVID-19 diagnosis was assessed using severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) laboratory tests and diagnosis codes. Incidence of COVID-19 per 100 000 encounters was estimated.

RESULTS
COVID-19 was diagnosed in 918 (0.1%) neonates (91.1 per 100 000 encounters [95% confidence interval 85.3–97.2]). Of these, 71 (7.7%) had severe infection (7 per 100 000 [95% confidence interval 5.5–8.9]). Median time to diagnosis was 14.5 days from birth (interquartile range 3.1–24.2). Common signs of infection were tachypnea and fever. Those with severe infection were more likely to receive respiratory support (50.7% vs 5.2%, P &lt; .001). Severely ill neonates received analgesia (38%), antibiotics (33.8%), anticoagulants (32.4%), corticosteroids (26.8%), remdesivir (2.8%), and COVID-19 convalescent plasma (1.4%). A total of 93.6% neonates were discharged home after care, 1.1% were transferred to another hospital, and discharge disposition was unknown for 5.2%. One neonate (0.1%) with presentation suggestive of multisystem inflammatory syndrome in children died after 11 days of hospitalization.

CONCLUSIONS
Most neonates infected with SARS-CoV-2 were asymptomatic or developed mild illness without need for respiratory support. Some had severe illness requiring treatment of COVID-19 with remdesivir and COVID-19 convalescent plasma. SARS-CoV-2 infection in neonates, though rare, may result in severe disease.
Development of high strength recycled aggregate concrete-composite effects of fly ash, silica fume and rice husk ash as pozzolans
Authors: Fernando Ashani , Selvaranjan Kajanan , Srikanth Gowsijan , Gamage J. C. P. H. 
Published: August 2022

AbstractThe world today has started facing bigger problems related to the concrete industry, especially with concrete becoming the most extensively used construction material in the world. At a time like this, where more eco-friendly substitutions are stringent, it calls for a more comprehensive approach in producing recycled concrete from recycled concrete aggregates (RCA). This study is an extension to previous studies involving composite utilization of pozzolans in treating RCA. It investigates on the possibility of using rice husk ash (RHA) along with other pozzolans as a cement replacement, in both stages of aggregate treatment and concrete production. It was observed that through this treatment, aggregate specific gravity was increased to a value of 2.37 which was earlier 2.18 for untreated RCA, 26% and 59% reductions were obtained for water absorption and porosity of aggregates, respectively. Further the concrete which contained RHA in both stages, attained a high strength of 55.4 MPa, even surpassing the control mix containing natural aggregates. The same mix resulted a 12% increase in its surface resistivity. Rapid Chloride Permeability Test (RCPT) and water permeability results also showed substantial improvements when compared to the reference RCA mix. Similar to previous studies involving composite use of pozzolans, improvements in the microstructure of both the aggregates and concrete through the use of RHA resulted in the amelioration of both mechanical and durability properties of concrete. This evidently indicates the possibility of achieving high strengths, even with the use of RCA derived from parent concretes of lower grades.
Problems of Mudharabah Financing in Islamic Banking After The Implementation of Qanun of Islamic Financial Institutions in Aceh
Authors: Yusmalinda Yusmalinda , Asmuni Asmuni , Tanjung Dhiauddin 
Published: August 2022

The mudharabah contract has become one of the Islamic banking products legitimated by The National Sharia Board and Financial Services Authority. The Aceh government, through the Qanun of Islamic Financial Institutions, has emphasized that banking practices in Aceh must rely on sharia principles. The consequence of this regulation is that banks are only allowed to use sharia contracts in every financial and financing transaction. This research is an empirical juridical study with a sociological and normative approach. This approach was employed to analyze the use of mudharabah contracts from both practical and theoretical aspects. The results of this study indicate that although the mudharabah contract has been designated as one of the financing products in Islamic banking, the mudharabah contract is not fully applied for financing. The mudharabah contract is only implemented for corporate purposes, not for small traders. This is due to several things, including the high risk in mudharabah financing, low bank confidence in customers, fluctuating profits, and low-risk management. This study aims to analyze the problems of mudharabah financing after implementing the 2018 Qanun of Islamic Financial Institutions in Aceh. The results of this study can answer the main problems in the mudharabah contracts. Thus the mudharabah contract can be optimized in the financing system of Islamic banking in Aceh.Akad mudharabah sudah menjadi salah satu produk perbankan syariah yang mendapatkan legitimasi dari DSN-MUI dan OJK. Pemerintah Aceh melalui qanun lembaga keuangan syariah telah menegaskan bahwa praktik perbankan di Aceh harus menggunakan prinsip-prinsip syariah. Konsekuensi dari peraturan tersebut yaitu pihak perbankan hanya dapat menggunakan akad bernuansa syariah dalam setiap transaksi keuangan dan pembiayaan. Penelitian ini merupakan kajian yuridis empiris dengan pendekatan sosiologi dan normatif. Pendekatan ini digunakan untuk menganalisis penggunaan akad mudhrabah baik dari aspek praktik dan teoritis. Hasil penelitian ini menunjukkan bahwa, meskipun akad mudharabah telah ditetapkan sebagai salah satu produk pembiayaan pada perbankan syariah, namun nyatanya akad mudharabah tidak sepenuhnya di gunakan dalam pembiayaan, akad mudharabah hanya digunakan untuk korporasi saja, tidak untuk pedagang kecil dan UMKM. Hal ini disebabkan oleh beberapa hal; pertama, tingginya resiko dalam pembiayaan mudharabah; kedua, rendahnya kepercayaan perbankan terhadap nasabah; ketiga, keuntungan yang fluktuatif; keempat, lemahnya manajemen resiko. Penelitian ini berkontribusi untuk mengatasi persoalan pembiayaan mudharabah pasca penerapan qanun lembaga keuangan syariah tahun 2018 di Aceh. Sehingga dengan hasil penelitian ini dapat menjadi jalan keluar terhadap permasalahan utama dalam akad mudharabah, dan akad mudharabah dapat dioptimalkan dalam sistem pembiayaan pada perbankan syariah di Aceh.
ThGM cells as a prognostic and predictive marker for coronary heart disease
Authors: Mou Dachao , wu shasha , Jiao Ling , Zhou Yi , Bai xiufeng 
Published: August 2022

Abstract
Background
Coronary heart disease (CHD) is causing by the aberrant aggregation of immune cells in plaque. This study aimed to identify abnormal T cell subtypes and inflammatory factors in CHD patients.
Methods
In total, 141 patients with CHD and 46 healthy controls at the West China hospital (Chengdu, China) between June 2020 and August 2020 were enrolled in this study. Patients were divided into five groups: Healthy control, SAP, UAP, AMI, and SCHD, based on the coronary angiography score and the clinical manifestations. Patients with any one of the following diseases were excluded: tumor, severe infectious disease, and severe inflammation. T cell subsets were analyzed using flow cytometry. Plasma concentration of cytokines were analyzed by Luminex.
Results
Flow cytometric analysis revealed that the number of ThGM cells was higher in CHD patients. The proportion of Th17 and Th1 cells were also increased in CHD patients. levels of IL-4, IL-5, IL-6, and IL-10 were significantly higher in CHD patients (P < 0.05). However, levels of GM-CSF were slightly lower in CHD patients. The multivariate analysis included 187 participants. Of these, 46 did not develop heart failure, 17 developed SCHD, 20 developed SAP, 69 developed UAP, and 35 developed AMI. In multivariate analysis, BMI, Hyperlipaemia, Hypertension, Total cholesterol, HDL, and LDL were not associated with CHD or GM-CSF expressing cell number. Using ANOVA for CHD clinical score studies. Using the student’s t test to assess between groups.
Conclusions
Our results suggested that ThGM can be considered as a diagnostic marker of CDH.
STMN2 overexpression promotes cell proliferation and EMT in pancreatic cancer mediated by WNT/β-catenin signaling
Authors: Wang Shiyang , Shao Mingrui , Wang Li , Zhang Qi , Wang Tianlong 
Published: August 2022

Abstract
STMN2, as a key regulator in microtubule disassembly and dynamics, has recently been reported to participate in the development of cancer. However, the corresponding role in pancreatic ductal adenocarcinoma (PC), to our knowledge has not been reported. We investigate the potential role of STMN2 in the progression of PC in vitro and vivo. Overexpression of STMN2 was prevalently observed in human PC tissues compared with that in paired pancreas (44/81,54.3% vs 15/81, 18.5%, P<0.01), which was positively with multiple advanced stage of PC patients (tumor size, T stage, lymph-node metastasis and the poor prognosis). Meanwhile, a close correlation between high STMN2 and cytoplasmic/nuclear β-catenin expression (P=0.007) was observed in PC tissues and cell lines. STMN2 overexpression induced EMT and cell proliferation in vitro, involving stimulation of EMT-like cellular morphology, cell motility and proliferation, and the change of EMT (Snail1, E-cad and Vimentin) and Cyclin D1 signaling. However, XAV939 inhibited STMN2 overexpression-enhanced EMT and proliferation. Conversely, KY19382 reversed STMN2 silencing- inhibited EMT and cell proliferation in vitro. Furthermore, activated STMN2 and β-catenin were co-localized in cytoplasm/nuclear in vitro. β-catenin/TCF-mediated the transcription of STMN2. Finally, STMN2 promoted subcutaneous tumor growth with the overexpression of EMT and Cyclin D1 signaling. STMN2 overexpression promotes aggressive clinical stage of PC patients and promotes EMT and cell proliferation in vitro and vivo. β-catenin/TCF-mediated the transcription of STMN2.
An experimental study in Real-time Facial Emotion Recognition on 3RL dataset
Authors: Zafra Rahmeh Abou , Abdullah Lana Ahmad , Alaraj Rouaa , Albezreh Rasha , Barhoum Tarek , Jallad Khloud Al 
Published: August 2022

Abstract
Although real-time facial emotion recognition is a hot topic research domain in the field of human-computer interaction, state-of-the-art available datasets still suffer from various problems, such as some unrelated photos such as document photos, unbalanced numbers of photos in each class, and misleading images that can negatively affect correct classification. The 3RL dataset was created, which contains approximately 24K images and will be publicly available, to overcome previously available dataset problems. The 3RL dataset is labelled with five basic emotions: happiness, fear, sadness, disgust, and anger. Moreover, we compared the 3RL dataset with other famous state-of-the-art datasets (FER dataset, CK + dataset), and we applied the most commonly used algorithms in previous works, SVM and CNN. The results show a noticeable improvement in generalization on the 3RL dataset. Experiments have shown an accuracy of up to 91.4% on 3RL dataset using CNN where results on FER2013, CK + are, respectively (approximately from 60–85%).
Arbuscular mycorrhizal fungi in oat-pea intercropping
Authors: Lee Alan , Neuberger Patrick , Omokanye Akim , Hernandez-Ramirez Guillermo , Kim Keunbae , Gorzelak Monika A. 
Published: August 2022

Abstract
Arbuscular mycorrhizal fungal diversity can be altered by intercropping plant species, as well as N fertilizer applications. This study examined the effects of oat-pea intercropping and N fertilizer addition on the richness and diversity of mycorrhizal species, as well as identified the most common arbuscular mycorrhizal fungi (AMF) genera recruited for oats and peas in two growing seasons (2019 and 2020). The AMF diversity was higher in an intercropped system compared to their respective monocropping system. Under drier conditions in 2019, arbuscular mycorrhizal richness decreased with N fertilizer addition in sole peas and increased with N fertilizer addition in sole oats, but no significant change in richness was observed in oat-pea intercropping. During the wetter growing season 2020, arbuscular mycorrhizal diversity increased when oat and pea were intercropped, compared to either sole oat or sole pea. Diversispora in sole pea was a significant indicator differentiating the root associated AMF community from sole oat. Claroideoglomus richness increased in peas in 2020, thus this genus could be moisture dependent. Paraglomus richness in oat-pea intercropping was similar to sole oat in 2019, and similar to sole pea in 2020. This can suggest that Paraglomus is an indicator of plant stress under intercropping, as based on the premise that stressed plants release more exudates, and the subsequent mycorrhizal associations favor these plants with higher exudation. Future investigations can further reveal the functions and benefits of these mycorrhizal genera in annual monocrop and intercropping systems.
Redox-dependent Structural Origins of the Temperature Threshold and Sensitivity in the TRPV3 Bio-thermometer
Authors: Wang Guangyu 
Published: August 2022

Abstract
The DNA hairpin with a limited size or strength is sensitive to temperature. However, it is unclear if thermosensitive TRP channels also use the temperature-dependent little hairpin sizes or strengths to govern their temperature thresholds and sensitivity. Here, graph theory was used as a novel tool to test this hypothesis by analyzing the redox- and state-dependent cryo-electron microscopy structures of mouse TRPV3 with or without the Y564A mutation at different temperatures. The results showed that the biggest little hairpin with the minimal topological loop size and strength determines the temperature threshold while a change in the total minimal little hairpin sizes upon a change in the total little hairpin-dependent non-covalent interactions along the channel gating pathway governs the temperature sensitivity. This size- and strength-dependent hairpin thermodynamics may tune the thermal activity and sensitivity of biological macromolecules. (136 words)SignificanceThe temperature thresholds and sensitivity of the thermosensitive transient receptor potential channels are often mediated by non-covalent interactions including H-bonds, salt bridges and aromatic p interactions at different ambient temperatures. Although their relative complexity has made them challenging to study, graph theory and hairpin thermodynamic modelling have now reached the stage at which we can explain their physical origins and gain reliable insight into the effects of the little hairpin size and strength on the thermo-stability of those non-covalent interactions along the channel gating pathway. This computational study offers opportunities for us to understand how temperature manipulates these complex non-covalent interactions and their direct incorporation into the design of bio-thermometers protects our bodies from noxious heat or cold damages. (119 words)Category: computational biochemistry; biophysics; graph theory; network analysis; redox regulation; structural bioinformatics
Design and Implementation of Two- Dimensional Pipelined&amp; Parallel FIR Filter Architectures
Authors: Singh Thingujam Churchil , Kumar Manoj 
Published: August 2022

Abstract
This paper aims at designing and implementation of two dimensional pipelining, fine-grain pipelining, parallel processing and combined pipelining and parallel processing architecture for FIR filter using VHDL (Very High Speed Integrated Circuit Hardware Description Language). These specific architectures significantly reduce the delay and the power consumption of the filter. Sampling frequency for the proposed designs is given in this paper. Rectangular window method is used for calculating Filter coefficients for the proposed two dimensional FIR filter architectures. The proposed filters are compared with the existing FIR filter in respect to area, operational speed, power consumption and delay. The proposed designs of the FIR digital filters provides an optimum output with 81% increase in speed of operation and up to 75% reduction in power consumption when compare to the existing architectures. Implementation of the FIR filter is carried out by using VHDL codes. Simulation and synthesis have been executed on Artix-7 FPGA series with the target device (xc7a35t-cpg236) speed grade-I using Xilinx Vivado 2015.2 version.
Long noncoding RNA ASH1L-AS1 promotes glycolysis and cisplatin resistance in ovarian cancer cells via its regulation on EZH2-mediated CNRIP1 methylation
Authors: Sun Xiao-Fei , Wang Yu , Yin Li-Li , Song Gui-Yu , Yang Qing 
Published: August 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Does secondary data suffice? Protected area conflict analysis in the era of the COVID-19 pandemic.
Authors: Rechciński Marcin , Tusznio Joanna , Akhshik Arash , Grodzińska-Jurczak Małgorzata 
Published: August 2022

Abstract
The COVID-19 pandemic has challenged protected area (PA) conflict analyses. A compelled shift towards broader use of secondary data evokes doubts about the validity of the results unless restrictive assessment procedures are implemented. To address this need, we propose a three-fold (theory-, method-, and cross-scale simulation-driven) approach to assess the usefulness of the utilized state register dataset and the indicator analysis methodology for the multi-level recognition of PA conflict determinants. With the ultimate aim to inform case study selection, we processed 187 relevant indicators from the Polish Central Statistical Office register for a Lesser Poland region. We distinguished five types of PA conflict determinants in Lesser Poland (‘urbanity’, ‘agriculture’, ‘tourism’, ‘small-scale entrepreneurship’, and ‘sprawl’) and respective groups of 15 clusters comprising local-level units. For one cluster we juxtaposed the obtained results with secondary data from another source (Internet content) and for a specific PA (Tatra National Park). Although the reported conflict issues corresponded with indicator-derived descriptors of the cluster, in the theory-driven phase of the assessment the state register failed to address the key prerequisites of PA conflicts. We proved that the proposed method can serve as a proxy for a multi-level PA conflict determinant analysis in crisis conditions such as COVID-19, provided it synthesizes the results of various methodological approaches, followed by in-person inquiries in the selected case studies.