bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical outcome and adherence rate in Scandinavian patients with intermediate‐intensity prophylaxis before and after the switch of standard half‐life FVIII products to BAY 81–8973
Authors: Arvanitakis Alexandros , Holme Pål Andre , Berntorp Erik , Astermark Jan 
Published: January 2022

AbstractIntroductionTreatment optimization in haemophilia A can be achieved by choice of FVIII product and knowledge of pharmacokinetics (PK), phenotype and adherence. A favourable PK profile of BAY 81–8973 (octocog alfa) (Kovaltry, Bayer AB) compared to other standard half‐life (SHL) FVIII products has been suggested.AimTo evaluate whether the switch to BAY 81–8973, using the same dosing schedule, impact factor consumption and bleed rates, taking arthropathy and adherence into accountMethodsForty patients on prophylaxis with SHL (median age 40.5 years) attending the haemophilia treatment centres in Malmö and Oslo were enrolled. The annualised bleeding rate (ABR) and joint bleeding rate (AJBR) before and after the switch to BAY 81–8973 was calculated. PK analyses were performed with WAPPS‐Hemo. Joint health status and treatment adherence were assessed.ResultsThe median ABR and AJBR was 0 before and after the switch, at both centres. The median yearly factor consumption was 3,345 IU/Kg/year in the entire study group corresponding to intermediate‐intensity prophylaxis in most patients and with significantly more used in Malmö (3,862 IU/Kg/year), compared to Oslo (2,337 IU/Kg/year) (P .006). There was no correlation between arthropathy and bleeding. The median BAY 81–8973 t½ was 15.15 h (range 7.5–29 h), with significant correlation to VWF levels, and 13.4 h after exclusion of VWF outliers. Adherence to treatment was 97%.ConclusionsConcentrate switch, using mainly intermediate‐intensity regimens with high adherence rates, preserves excellent prophylaxis outcome using standard half‐life FVIII products, indicating the value of individualized prophylaxis and close follow‐up.
Prevalence estimates of Helicobacter species infection in pancreatic and biliary tract cancers
Authors: Osaki Takako , Lin Yingsong , Sasahira Naoki , Ueno Makoto , Yonezawa Hideo , Hojo Fuhito , Okuda Masumi , Matsuyama Masato , Sasaki Takashi , Kobayashi Satoshi , Tezuka Shun , Tanaka Kei , Dan Naoaki , Kuruma Sawako , Egawa Naoto , Kamiya Shigeru , Kikuchi Shogo 
Published: January 2022

AbstractBackgroundHelicobacter pylori infection is a well‐established risk factor for gastric cancer and has been linked to other gastrointestinal diseases, including pancreatic and biliary tract cancers; however, the relevance of enterohepatic non‐H. pylori helicobacters to the pathophysiology of these diseases remains unclear.Materials and MethodsWe estimated the prevalence of two enterohepatic non‐H. pylori helicobacters (Helicobacter hepaticus and Helicobacter bilis) in the framework of a hospital‐based case‐control study involving 121 patients with biliary tract cancer, pancreatic cancer, or other gastrointestinal diseases. Bile and blood samples were collected from the patients undergoing endoscopic retrograde cholangiopancreatography. The presence of H. bilis, H. hepaticus, and other Helicobacter spp. was examined using bacterial culture, PCR‐based detection, and serological tests.ResultsCulture of Helicobacter spp. from biliary brush samples was unsuccessful. Approximately 13.0% (15/115) of the bile samples collected from patients with a variety of gastrointestinal cancers, including pancreatic and biliary tract cancers, tested positive for one of the enterohepatic non‐H. pylori helicobacter species as determined by PCR. Specifically, H. bilis and H. hepaticus DNA were detected in 11 and 4 bile samples, respectively. Approximately 20%–40% of the patients tested positive for serum non‐H. pylori helicobacter IgG antibodies. The seroprevalence of H. bilis and H. hepaticus in the patients without evidence of H. pylori infection appeared to be higher in the pancreatic cancer group than in the control group.ConclusionOur findings suggest a role for Helicobacter spp., especially H. bilis and H. hepaticus, in the etiology of pancreatic and biliary tract cancers.
A comparison of wild bee communities in sown flower strips and semi‐natural habitats: A pollination network approach
Authors: Hadrava Jiří , Talašová Anna , Straka Jakub , Benda Daniel , Kazda Jan , Klečka Jan 
Published: January 2022

Abstract
Drastic reductions of insect diversity and abundance have been observed in highly fragmented agricultural landscapes of central Europe. Declines of pollinators may have detrimental effects on the reproduction of wild insect‐pollinated plants as well as the yield of crops. In order to mitigate such impacts, sown flower strips on arable land within Agri‐Environment Climate Schemes (AECS) are supported across EU countries. However, it is not clear whether sown flower strips provide equivalent benefits to wild flower‐visiting insects as semi‐natural habitats.
Here, we apply plant–pollinator network approach to evaluate the function of sown flower strips for the communities of wild bees. We compared the structural characteristics and the robustness of plant–pollinator networks in sown flower strips and nearby semi‐natural habitats in seven sites in the Czech Republic. We also quantified the importance of individual plant species for bees based on simulations of plant–pollinator extinction cascades.
We found that assemblages of plants and pollinators were less diverse in sown flower strips than in semi‐natural habitats, more generalised, and more nested. However, we did not find any significant differences in network robustness to plant–pollinator coextinctions. Further, simulations revealed large variation in the functional importance among plant species from both habitats.
We conclude that although the analysis of network robustness suggested that plants in the sown flower strips and semi‐natural habitats were functionally equivalent, this masked important differences between the two habitats. From a conservation point of view, semi‐natural habitats were superior in supporting a more diverse community of solitary bees and bumblebees, likely because of their greater longevity and higher habitat heterogeneity.
Current knowledge, attitude and practice among dental hygienists in oral cancer awareness: Systematic review
Authors: Coppola Noemi , Rivieccio Immacolata , Blasi Andrea , Ferrigno Roberto , Baldares Stefania , Mignogna Michele Davide , Leuci Stefania 
Published: January 2022

AbstractObjectivesEvidence on the awareness and knowledge level of oral cancer and its associated risk factors among dental hygienists is scarce; this systematic review aimed to synthesize their available evidence of the level of knowledge, attitude and practice.MethodsPubMed and Scopus were searched for publications from any year up to January 2021. Studies about knowledge and/or attitudes and/or practices of dental hygienists have been taken into account. Overall, 14 studies have been selected for the systematic review.ResultsExcluding tobacco use (99.8%–100%), considerable variability were found among dental hygienists about important oral cancer risk factors such as alcohol consumption (30.0%–90.0%), human papilloma virus (23.0%–90.0%), oldness (37.7%–69.3%), diet (30.0%–42.2%) and betel quid chewing (5.0% and 98.0%). There was a good level of awareness among dental hygienists regarding leukoplakia (86.5%), instead less than half recognized erythroplakia as a precancerous lesion. Moderate knowledge was recorded about frequent sites of oral cancer development. Most of dental hygienists reported to perform intraoral screening (85.2%–100%). To regard attitude, a great variability was found about adequacy of undergraduate training (15.7%–75.0%) and most of dental hygienists expressed the need for continuing education (92.7%–99.0%).ConclusionsDental hygienists play a key role in oral cancer detection. Low knowledge of oral cancer among dental hygienists is strongly associated with the low levels of early detection. These findings provide useful information to improve continuing education programmes pre‐ and post‐graduation targeted at the prevention of oral cancer in order to reduce oral cancer morbidity and mortality.
Improving the drying kinetics and microstructure of vacuum‐fried ripened durian chips
Authors: Thongcharoenpipat Chanoknate , Yamsaengsung Ram 
Published: January 2022

SummaryThis research evaluated the effects of process conditions on the physiochemical properties of vacuum‐fried durian chips. Ripened durians were sliced and fried at 90, 100, 110 and 120°C under 8‐kPa absolute pressure. From this study, microwave heating (MWH) during vacuum frying (VF) significantly reduced the frying time by 20% compared to VF (P < 0.05) with the effective moisture diffusivity for VF, pre‐treatment before vacuum frying (P+VF) and VF+MWH at 110°C determined to be 3.63 × 10−09, 4.30 × 10−09 and 4.84 × 10−09 m2 s−1, respectively. VF+MWH produced chips with significantly lower shrinkage, more yellowness (P < 0.05) and higher crispiness (consistent with the microstructure from scanning electron microscopy (SEM)). In addition, VF+MWH drastically decreased oil absorption by 10%, while P+VF yielded the lowest specific energy consumption (SEC). From sensory evaluation, the overall acceptability of VF+MWH chips rose from 5.83 to 6.07 (seven‐point hedonic scale), thus making the product fall into the ‘Good’ to ‘Very Good’ range.
Fermentation of legumes with Rhizopus oligosporus: effect on physicochemical, functional and microstructural properties
Authors: Toor Barinderjeet Singh , Kaur Amarjeet , Kaur Jaspreet 
Published: January 2022

SummaryThe present study investigated the effect of fermentation by Rhizopus oligosporus on the nutritional, functional, colour and microstructural properties of some commonly consumed legumes, that is, chickpea (kabuli and desi), pigeon pea and soybean. Results revealed a substantial (P < 0.05) increment in the protein (5–14%) and ash (5–15%) contents as well as those of essential (13–21%) and non‐essential (3–15%) amino acids with fermentation. Fatty acid composition showed the varied concentrations of polyunsaturated, monounsaturated and saturated fatty acids in fermented legumes. Further, fermentation significantly (P < 0.05) modified the functional and colour properties of legumes. The alteration in the structural conformation of legumes upon fermentation was observed with scanning electron microscopy which confirmed the enzymatic action on the proteins, fibres and lipid bodies. The results concluded that fermentation of these legumes could be a potential approach in developing the novel functional foods to address the concerns of food and nutrition securities.
Genetic characterization of influenza A virus subtypes H11N6, H11N7, and H11N9 isolated from free‐grazing ducks, Thailand
Authors: Chaiyawong Supassama , Charoenkul Kamonpan , Udom Kitikhun , Chamsai Ekkapat , Jairak Waleemas , Boonyapisitsopa Supanat , Bunpapong Napawan , Amonsin Alongkorn 
Published: January 2022

AbstractInfluenza A viruses (IAVs) infect avian species and several mammalian species including humans. Anseriformes water birds are an important reservoir of IAVs. In this study, we identified and characterized IAV subtypes H11N6 (n = 5), H11N7 (n = 3), and H11N9 (n = 3) isolated during the influenza surveillance program in free‐grazing ducks from 2012 to 2015 in Thailand. Eleven IAV‐H11 viruses were characterized by either whole genome sequencing (n = 5) or HA and NA gene sequencing (n = 6) for phylogenetic and amino acid analyses. Phylogenetic analysis showed that Thai IAV‐H11 were grouped into Avian Eurasian lineage. Amino acid analysis showed that all Thai IAV‐H11 viruses have low pathogenic avian influenza (LPAI) characteristics and sensitive to Oseltamivir and Amantadine. Novel reassortant viruses (IAV‐H11N7 and IAV‐H11N9) have been observed. The reassortant viruses contained NP, M, and NS gene segments which originate from intercontinental sources which never been reported in Thai IAVs. In summary, this study demonstrated high genetic diversity of IAV‐H11 circulating in free‐grazing ducks. Free‐grazing ducks infected with IAVs generated novel reassortant IAV‐H11. Thus, surveillance of IAVs in free‐grazing ducks should be routinely conducted to monitor novel reassortant viruses and subsequently potential virulence viruses.
Porous ceramics with near‐zero shrinkage and low thermal conductivity from hazardous secondary aluminum dross
Authors: Li Wenhao , Zhang Xiaoyan , Zhang Junjie , Shen Hanlin , Yang Junjie , Liu Ying , Liu Jun , Zhang Shengen , Yang Jinlong 
Published: January 2022

AbstractHerein, a simple, versatile, and low‐cost approach has been proposed to realize the green utilization of secondary aluminum dross, the hazardous solid waste, namely directly sintering dry‐pressed green bodies from secondary aluminum dross to fabricate porous ceramics according to high‐temperature foaming process spontaneously without adding spare foaming agents. Aluminum nitride (AlN) in secondary aluminum dross was employed to realize high‐temperature foaming due to its oxidation, which makes traditional AlN and salts removal process needless. Moreover, near‐zero shrinkage or even expansion during sintering of porous ceramics have occurred because in‐situ foaming process together with the oxidation of Al particles well offset the sintering shrinkage. After sintering at 1400°C for 2 h, porous ceramics composed of α‐Al2O3 and spinel phases with open porosity of 37.91%, sintering expansion rate of 1.13%, flexural strength of 45.67 MPa, and thermal conductivity of 0.97 W/(m·K) have been prepared. Cenospheres as pore‐forming agents have been added to further improve the porosity, and alumina‐based porous ceramics with open porosity of 28.39%–43.20% and flexural strength of 15.80–52.48 MPa have been obtained. This effective solution for recycling secondary aluminum dross could supply high‐performance porous ceramics, which is expected to be applied in the fields of light‐weight structural components and thermal insulations.
Excellent thermal stability and energy storage properties of lead‐free Bi0.5Na0.5TiO3‐based ceramic
Authors: Jiang Zehua , Yuan Ying , Yang Hongcheng , Li Enzhu , Zhang Shuren 
Published: January 2022

AbstractThe ceramic capacitors with excellent energy storage properties and wide operating temperature are the main challenges in power system applications. Here, the lead‐free (1‐x)Bi0.5Na0.5TiO3‐xCaTiO3 (abbreviated as BNT‐xCT) ceramics were synthesized through solid‐state reaction method. The introduction of CT reduced the temperature of permittivity peak of BNT ceramic, guaranteeing excellent thermal stability over a wide temperature range of −100 ∼ 136°C. Meanwhile, the long‐range order structure of BNT was destructed by structural distortion, and the relaxor behavior was enhanced after doping CT. Moreover, the direct current breakdown strength was improved from 203 to 455 kV/cm, and the high recoverable energy density (Wrec ∼ 2.74 J/cm3) with high efficiency (η ∼ 91%) was achieved for BNT‐0.25CT ceramic, along with a fast discharge speed (t0.9 ∼ 110 ns) superior cycle stability and thermal stability. Those properties enabled a promising practical prospect of BNT‐CT ceramics.
The combination of hexanal and geraniol in sublethal concentrations synergistically inhibits quorum sensing in Pseudomonas fluorescens—In vitro and in silico approaches
Authors: Yu Hang , Liu Yu , Yang Fangwei , Xie Yunfei , Guo Yahui , Cheng Yuliang , Yao Weirong 
Published: January 2022

Abstract

Aim
Hexanal and geraniol are essential oil components with anti-quorum sensing (QS) activity against Pseudomonas fluorescens. This study demonstrated that QS inhibition (QSI) efficacy of the hexanal and geraniol combination (HG) was significantly higher when compared to those of their mono-counterparts at the same concentration.

Methods and Results
Tests on P. fluorescens motility, biofilm formation, acyl-homoserine lactones’ (AHLs) production, gene expression in vitro, and molecular docking in silico were conducted to evaluate the synergistic effect of hexanal and geraniol on QSI. HG mixture at 0.5 minimal inhibitory concentration (MIC) showed a strong synergistic inhibition of biofilm formation (51.8%), motility (60.13%), and extracellular protease activity (58.9%) of P. fluorescens. The synthesis of AHLs, e.g., C8-HSL and C12-HSL, was inhibited by hexanal, geraniol, and HG; both AHLs are responsible for regulating virulence factors in P. fluorescens. The expression of pcoI and gacA genes regulating AHL synthetase and sensor kinase was significantly down-regulated by HG (0.29 and 0.38-fold) at 0.5 MIC. Hexanal and HG showed significant inhibition of the expression of pcoR and gacS genes, which are responsible for AHL receptor protein and response regulation; however, geraniol failed to downregulate the two genes. Molecular docking in silico also supported these findings. Hexanal, which gets inserted into the minor groove of pcoI/pcoR DNA fragments, inhibits the expression of both the genes. Both hexanal (−31.487 kcal/mol) and geraniol (−25.716 kcal/mol) had a higher binding affinity with PcoI protein than the halogenated furanone C30 (−24.829 kcal/mol), which is a known competitor of AHLs. Similarly, hexanal and geraniol strongly bind to the PcoR protein also.

Conclusions
It was found that HG at 0.5 MIC could effectively inhibit QS by suppressing the expression of pcoR/gacS and gacA/gacS genes and therefore, could inhibit the motility and biofilm formation of P. fluorescens.

Significance and Impact of the Study
The present study indicated that HG at sub-MIC as QS inhibitor could be further developed as a new preservative of agri-food products.
Melatonin ameliorates paclitaxel‐induced mice spermatogenesis and fertility defects
Authors: Wang ZhiXin , Teng Zi , Wang ZeLin , Song Zhan , Zhu Peng , Li Ning , Zhang YuSheng , Liu XueXia , Liu FuJun 
Published: January 2022

AbstractChemotherapeutic drug of paclitaxel (PTX) has been shown to cause reproductive toxicity thus affecting male fertility, but its underlying molecular basis is unclear. Melatonin (MLT) can mitigate the reproductive damage caused by certain chemotherapy drugs. In this study, we aimed to identify impact of PTX on the main biological processes and protective effect of MLT on reproductive damage caused by PTX. Mice exposed to PTX mainly impaired spermatogenesis, such as decreased sperm counts, reduced sperm motility and increased abnormal sperm. Decreased expressions of germ cell proliferation‐associated protein PCNA and meiosis‐related protein SYCP3 induced by PTX were determined by Western blot, while MLT ameliorated this effect and increased the expressions of PCNA, SYCP3, DMC1, STRA8 and fertility‐related protein of HSPA2, resulting in significantly improved spermatogenesis and sperm quality levels. In vitro fertilization experiment showed that PTX significantly decreased blastocyst formation rates, which can be improved by MLT administration, but not two‐cell development rates. Taken together, this work demonstrated PTX can adversely affect germ cell proliferation and meiosis, which ultimately influence sperm quality and male fertility, and highlighted the protective ability of MLT on ameliorating the side effects of PTX, especially on sperm quality. The results provide information to further the study on the molecular mechanism of PTX's effects on male reproduction and the protective mechanism of MLT.
Prevalence and molecular subtyping of Blastocystis in domestic pigeons in Henan Province, Central China
Authors: Su Changwei , Mei Xuefang , Wei Lai , Wang Jiawen , Feng Xia , Wang Pei , He Bo , Chang Yuan , Xu Fuyang , Wang Mingyong , Tian Xiaowei , Zhang Zhenchao , Li Xiangrui , Wang Shuai 
Published: January 2022

Abstract
Blastocystis is an anaerobic intestinal protozoan parasite found in humans and many kinds of animals that mainly causes diarrhea, abdominal pain, and other clinical symptoms. At present, research on the prevalence and subtype diversity of Blastocystis in domestic pigeons is very limited. The purpose of this study was to detect the infection rate and gene subtype distribution of Blastocystis in domestic pigeons in Henan Province, Central China, to provide a foundation for preventing and controlling Blastocystis in domestic pigeons. Fecal DNA was extracted from 504 fresh fecal samples of pigeons collected from four areas in Henan Province, Central China. All DNA samples were investigated by polymerase chain reaction, and positive samples were sequenced to analyze the gene subtypes based on small ribosomal subunit (SSU rRNA) gene. The overall infection rate of Blastocystis in pigeons in Henan Province was 7.7% (39/504). Four subtypes (STs) of Blastocystis were identified including ST1 (2/39, 5.1%), ST3 (16/39, 41%), ST4 (1/39, 2.6%), and ST7 (20/39, 51.3%), all of which belonged to zoonotic subtypes, and ST7 was the dominant gene subtype. The results show that Blastocystis infection is common in domestic pigeons in Henan Province, Central China, and the pathogens were zoonotic subtypes. Particular attention should be given to reducing the risk of transmission of Blastocystis from domestic pigeons to humans.
Clinical Anti‐aging Efficacy of Propolis Polymeric Nanoparticles Prepared by a Temperature‐induced Phase Transition Method
Authors: An Joo Young , Kim Chaejin , Park Na Rae , Jung Han Soo , Koo Tae‐sung , Yuk Soon Hong , Lee Eun Hee , Cho Sun Hang 
Published: January 2022

AbstractBackgroundCollagen forms a dermal matrix in the skin. Biosynthesis and decomposition of collagen are the major processes in skin aging. Propolis is rich in flavonoids and phenolic compounds, which are known to be effective in preventing skin aging, including the enhancement of fibroblast proliferation, activation, and growth capacity.ObjectivesThe aim of this study was to develop a poorly soluble propolis extract as an active ingredient in cosmetic products for anti‐aging efficacy.Methods & ResultsPolymeric nanoparticles containing propolis extract, polyethylene glycol 400, and poloxamer 407 were prepared via a temperature‐induced phase transition method. The particle size of the polymeric nanoparticles was approximately 20.75 nm. The results of an in vitro procollagen type I carboxy‐terminal peptide assay and a matrix metalloproteinase‐1 inhibition assay showed that the polymeric nanoparticles increased collagen production by 19.81%–24.59% compared to blank (p < 0.05), and significantly reduced intracellular collagenase activity by 7.46%–31.52% compared to blank (p < 0.05). In a clinical trial, polymeric nanoparticles in a cosmetic formulation were applied around the eyes of 24 female subjects for 8 weeks. Five skin parameters were significantly improved after the application of the test ampoule. Visual evaluation using the Global Photo Damage Score showed a significant reduction in wrinkles after the application of the test ampoules (p < 0.001).ConclusionsThis study outlines the development of stable polymeric nanoparticles containing poorly soluble propolis in a cosmetic formulation, and its efficacy in wrinkle improvement. The developed polymeric nanoparticles were effective for alleviating wrinkles and can be used for pharmaceutical applications that utilize propolis as antiseptic, anti‐inflammatory, antimycotic, antifungal, antibacterial, antiulcer, anticancer, and immunomodulatory agents.