bluuebunny/crossref_metadata_2025_split huggingface dataset:
OXTRHigh stroma fibroblasts control the invasion pattern of oral squamous cell carcinoma via ERK5 signaling
Authors: Ding Liang , Fu Yong , Zhu Nisha , Zhao Mengxiang , Ding Zhuang , Zhang Xiaoxin , Song Yuxian , Jing Yue , Zhang Qian , Chen Sheng , Huang Xiaofeng , O’Reilly Lorraine A , Silke John , Hu Qingang , Ni Yanhong 
Published: August 2022

AbstractThe Pattern Of Invasion (POI) of tumor cells into adjacent normal tissues clinically predicts postoperative tumor metastasis/recurrence of early oral squamous cell carcinoma (OSCC), but the mechanisms underlying the development of these subtypes remain unclear. Focusing on the highest score of POIs (Worst POI, WPOI) present within each tumor, we observe a disease progression-driven shift of WPOI towards the high-risk type 4/5, associated with a mesenchymal phenotype in advanced OSCC. WPOI 4-5-derived cancer-associated fibroblasts (CAFsWPOI4-5), characterized by high oxytocin receptor expression (OXTRHigh), contribute to local-regional metastasis. OXTRHighCAFs induce a desmoplastic stroma and CCL26 is required for the invasive phenotype of CCR3+tumors. Mechanistically, OXTR activates nuclear ERK5 transcription signaling via Gαq and CDC37 to maintain high levels of OXTR and CCL26. ERK5 ablation reprograms the pro-invasive phenotype of OXTRHighCAFs. Therefore, targeting ERK5 signaling in OXTRHighCAFs is a potential therapeutic strategy for OSCC patients with WPOI 4-5.
Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation
Authors: Hu Wenqi , Zeng Haitao , Shi Yanan , Zhou Chuanchuan , Huang Jiana , Jia Lei , Xu Siqi , Feng Xiaoyu , Zeng Yanyan , Xiong Tuanlin , Huang Wenze , Sun Peng , Chang Yajie , Li Tingting , Fang Cong , Wu Keliang , Cai Lingbo , Ni Wuhua , Li Yan , Yang Zhiyong , Zhang Qiangfeng Cliff , Chian RiCheng , Chen Zijiang , Liang Xiaoyan , Kee Kehkooi 
Published: August 2022

AbstractThe combined use of transcriptome and translatome as indicators of gene expression profiles is usually more accurate than the use of transcriptomes alone, especially in cell types governed by translational regulation, such as mammalian oocytes. Here, we developed a dual-omics methodology that includes both transcriptome and translatome sequencing (T&T-seq) of single-cell oocyte samples, and we used it to characterize the transcriptomes and translatomes during mouse and human oocyte maturation. T&T-seq analysis revealed distinct translational expression patterns between mouse and human oocytes and delineated a sequential gene expression regulation from the cytoplasm to the nucleus during human oocyte maturation. By these means, we also identified a functional role of OOSP2 inducing factor in human oocyte maturation, as human recombinant OOSP2 induced in vitro maturation of human oocytes, which was blocked by anti-OOSP2. Single-oocyte T&T-seq analyses further elucidated that OOSP2 induces specific signaling pathways, including small GTPases, through translational regulation.
The emerging role of drought as a regulator of dissolved organic carbon in boreal landscapes
Authors: Tiwari Tejshree , Sponseller Ryan A. , Laudon Hjalmar 
Published: August 2022

AbstractOne likely consequence of global climate change is an increased frequency and intensity of droughts at high latitudes. Here we use a 17-year record from 13 nested boreal streams to examine direct and lagged effects of summer drought on the quantity and quality of dissolved organic carbon (DOC) inputs from catchment soils. Protracted periods of drought reduced DOC concentrations in all catchments but also led to large stream DOC pulses upon rewetting. Concurrent changes in DOC optical properties and chemical character suggest that seasonal drying and rewetting trigger soil processes that alter the forms of carbon supplied to streams. Contrary to expectations, clearest drought effects were observed in larger watersheds, whereas responses were most muted in smaller, peatland-dominated catchments. Collectively, our results indicate that summer drought causes a fundamental shift in the seasonal distribution of DOC concentrations and character, which together operate as primary controls over the ecological and biogeochemical functioning of northern aquatic ecosystems.
Smart low interfacial toughness coatings for on-demand de-icing without melting
Authors: Azimi Dijvejin Zahra , Jain Mandeep Chhajer , Kozak Ryan , Zarifi Mohammad H. , Golovin Kevin 
Published: August 2022

AbstractIce accretion causes problems in vital industries and has been addressed over the past decades with either passive or active de-icing systems. This work presents a smart, hybrid (passive and active) de-icing system through the combination of a low interfacial toughness coating, printed circuit board heaters, and an ice-detecting microwave sensor. The coating’s interfacial toughness with ice is found to be temperature dependent and can be modulated using the embedded heaters. Accordingly, de-icing is realized without melting the interface. The synergistic combination of the low interfacial toughness coating and periodic heaters results in a greater de-icing power density than a full-coverage heater system. The hybrid de-icing system also shows durability towards repeated icing/de-icing, mechanical abrasion, outdoor exposure, and chemical contamination. A non-contact planar microwave resonator sensor is additionally designed and implemented to precisely detect the presence or absence of water or ice on the surface while operating beneath the coating, further enhancing the system’s energy efficiency. Scalability of the smart coating is demonstrated using large (up to 1 m) iced interfaces. Overall, the smart hybrid system designed here offers a paradigm shift in de-icing that can efficiently render a surface ice-free without the need for energetically expensive interface melting.
Large contribution of fossil-derived components to aqueous secondary organic aerosols in China
Authors: Xu Buqing , Zhang Gan , Gustafsson Örjan , Kawamura Kimitaka , Li Jun , Andersson August , Bikkina Srinivas , Kunwar Bhagawati , Pokhrel Ambarish , Zhong Guangcai , Zhao Shizhen , Li Jing , Huang Chen , Cheng Zhineng , Zhu Sanyuan , Peng Pingan , Sheng Guoying 
Published: August 2022

AbstractIncomplete understanding of the sources of secondary organic aerosol (SOA) leads to large uncertainty in both air quality management and in climate change assessment. Chemical reactions occurring in the atmospheric aqueous phase represent an important source of SOA mass, yet, the effects of anthropogenic emissions on the aqueous SOA (aqSOA) are not well constrained. Here we use compound-specific dual-carbon isotopic fingerprints (δ13C and Δ14C) of dominant aqSOA molecules, such as oxalic acid, to track the precursor sources and formation mechanisms of aqSOA. Substantial stable carbon isotope fractionation of aqSOA molecules provides robust evidence for extensive aqueous-phase processing. Contrary to the paradigm that these aqSOA compounds are largely biogenic, radiocarbon-based source apportionments show that fossil precursors produced over one-half of the aqSOA molecules. Large fractions of fossil-derived aqSOA contribute substantially to the total water-soluble organic aerosol load and hence impact projections of both air quality and anthropogenic radiative forcing. Our findings reveal the importance of fossil emissions for aqSOA with effects on climate and air quality.
The importance and mitigation of mycotoxins and plant toxins in Southeast Asian fermented foods
Authors: Owolabi Iyiola O. , Kolawole Oluwatobi , Jantarabut Phantakan , Elliott Christopher T. , Petchkongkaew Awanwee 
Published: August 2022

AbstractFermented foods (ffs) and beverages are widely consumed in Southeast Asia (SEA) for their nutritional balance, flavor, and food security. They serve as vehicles for beneficial microorganisms performing a significant role in human health. However, there are still major challenges concerning the safety of ffs and beverages due to the presence of natural toxins. In this review, the common toxins found in traditional ffs in SEA are discussed with special reference to mycotoxins and plant toxins. Also, mitigation measures for preventing risks associated with their consumption are outlined. Ochratoxin, citrinin, aflatoxins were reported to be major mycotoxins present in SEA ffs. In addition, soybean-based ff food products were more vulnerable to mycotoxin contaminations. Common plant toxins recorded in ffs include cyanogenic glycosides, oxalates, phytates and saponins. Combined management strategies such as pre-harvest, harvest and post-harvest control and decontamination, through the integration of different control methods such as the use of clean seeds, biological control methods, fermentation, appropriate packaging systems, and controlled processing conditions are needed for the safe consumption of indigenous ffs in SEA.
African-specific molecular taxonomy of prostate cancer
Authors: Jaratlerdsiri Weerachai , Jiang Jue , Gong Tingting , Patrick Sean M. , Willet Cali , Chew Tracy , Lyons Ruth J. , Haynes Anne-Maree , Pasqualim Gabriela , Louw Melanie , Kench James G. , Campbell Raymond , Horvath Lisa G. , Chan Eva K. F. , Wedge David C. , Sadsad Rosemarie , Brum Ilma Simoni , Mutambirwa Shingai B. A. , Stricker Phillip D. , Bornman M. S. Riana , Hayes Vanessa M. 
Published: August 2022

AbstractProstate cancer is characterized by considerable geo-ethnic disparity. African ancestry is a significant risk factor, with mortality rates across sub-Saharan Africa of 2.7-fold higher than global averages1. The contributing genetic and non-genetic factors, and associated mutational processes, are unknown2,3. Here, through whole-genome sequencing of treatment-naive prostate cancer samples from 183 ancestrally (African versus European) and globally distinct patients, we generate a large cancer genomics resource for sub-Saharan Africa, identifying around 2 million somatic variants. Significant African-ancestry-specific findings include an elevated tumour mutational burden, increased percentage of genome alteration, a greater number of predicted damaging mutations and a higher total of mutational signatures, and the driver genes NCOA2, STK19, DDX11L1, PCAT1 and SETBP1. Examining all somatic mutational types, we describe a molecular taxonomy for prostate cancer differentiated by ancestry and defined as global mutational subtypes (GMS). By further including Chinese Asian data, we confirm that GMS-B (copy-number gain) and GMS-D (mutationally noisy) are specific to African populations, GMS-A (mutationally quiet) is universal (all ethnicities) and the African–European-restricted subtype GMS-C (copy-number losses) predicts poor clinical outcomes. In addition to the clinical benefit of including individuals of African ancestry, our GMS subtypes reveal different evolutionary trajectories and mutational processes suggesting that both common genetic and environmental factors contribute to the disparity between ethnicities. Analogous to gene–environment interaction—defined here as a different effect of an environmental surrounding in people with different ancestries or vice versa—we anticipate that GMS subtypes act as a proxy for intrinsic and extrinsic mutational processes in cancers, promoting global inclusion in landmark studies.
Mapping the association between tau-PET and Aβ-amyloid-PET using deep learning
Authors: Ruwanpathirana Gihan P. , Williams Robert C. , Masters Colin L. , Rowe Christopher C. , Johnston Leigh A. , Davey Catherine E. 
Published: August 2022

AbstractIn Alzheimer’s disease, the molecular pathogenesis of the extracellular Aβ-amyloid (Aβ) instigation of intracellular tau accumulation is poorly understood. We employed a high-resolution PET scanner, with low detection thresholds, to examine the Aβ-tau association using a convolutional neural network (CNN), and compared results to a standard voxel-wise linear analysis. The full range of Aβ Centiloid values was highly predicted by the tau topography using the CNN (training R2 = 0.86, validation R2 = 0.75, testing R2 = 0.72). Linear models based on tau-SUVR identified widespread positive correlations between tau accumulation and Aβ burden throughout the brain. In contrast, CNN analysis identified focal clusters in the bilateral medial temporal lobes, frontal lobes, precuneus, postcentral gyrus and middle cingulate. At low Aβ levels, information from the middle cingulate, frontal lobe and precuneus regions was more predictive of Aβ burden, while at high Aβ levels, the medial temporal regions were more predictive of Aβ burden. The data-driven CNN approach revealed new associations between tau topography and Aβ burden.
A retrospective observational study analyzing work and study motivation based on the work environment of 15,677 Japanese clinicians in 2016
Authors: Shimazu Yuzo , Kobashi Yurie , Imoto Seiya , Tsubokura Masaharu 
Published: August 2022

AbstractPhysicians play an active role in public health. However, there is a limit to the knowledge and experience that can be gained through hospital work alone. This was a secondary data analysis from 100,000 doctors in Japan (15,677 respondents). The results of the analysis showed that 898 (8.4%) male and 190 (6.0%) female doctors worked 60 h or more in a week. The percentage of physicians whose spouse was a physician was found to be 31.4% (male) and 61.7% (female) (p < 0.001), and the rate of full-time working clinicians was 85.7% (male) and 30.0% (female) (p < 0.001). In the univariate analysis, female’s working hours were affected by childbirth and childcare experience (p < 0.001, 95% CI − 10.3 to − 8.4, with “none” as reference) and specialty certification (p < 0.001, 95% CI − 3.5 to − 1.4, with “none” as reference). In the multivariate analysis, physician’s working hours were associated with sex (coefficient, − 7.4; 95% CI − 8.3 to − 6.5, with “male as reference), childbirth/childcare (coefficient, − 2.2; 95% CI − 2.9 to − 1.4, with “possession” as reference), and specialty qualification (coefficient − 4.0, 95% CI − 5.0 to − 3.0, with “possession” as reference). To summarize the results of the analysis, work/study motivation of physicians will be facilitated by ensuring adequate learning opportunities and by developing support systems and environments.
Environment-specificity and universality of the microbial growth law
Authors: Wang Qirun , Lin Jie 
Published: August 2022

AbstractAs the nutrient quality changes, the fractions of ribosomal proteins in the proteome are usually positively correlated with the growth rates due to the auto-catalytic nature of ribosomes. While this growth law is observed across multiple organisms, the relation between the ribosome fraction and growth rate is often more complex than linear, beyond models assuming a constant translation speed. Here, we propose a general framework of protein synthesis considering heterogeneous translation speeds and protein degradations. We demonstrate that the growth law curves are generally environment-specific, e.g., depending on the correlation between the translation speeds and ribosome allocations among proteins. Our predictions of ribosome fractions agree quantitatively with data of Saccharomyces cerevisiae. Interestingly, we find that the growth law curve of Escherichia coli nevertheless appears universal, which we prove must exhibit an upward bending in slow-growth conditions, in agreement with experiments. Our work provides insights on the connection between the heterogeneity among genes and the environment-specificity of cell behaviors.
Improved seasonal prediction of harmful algal blooms in Lake Erie using large-scale climate indices
Authors: Tewari Mukul , Kishtawal Chandra M. , Moriarty Vincent W. , Ray Pallav , Singh Tarkeshwar , Zhang Lei , Treinish Lloyd , Tewari Kushagra 
Published: August 2022

AbstractHarmful Algal Blooms lead to multi-billion-dollar losses in the United States due to shellfish closures, fish mortalities, and reluctance to consume seafood. Therefore, an improved early seasonal prediction of harmful algal blooms severity is important. Conventional methods for harmful algal blooms prediction using nutrient loading as the primary driver have been found to be less accurate during extreme bloom years. Here we show that a machine learning approach using observed nutrient loading, and large-scale climate indices can improve the harmful algal blooms prediction in Lake Erie. Moreover, the seasonal prediction of harmful algal blooms can be completed by early June, before the expected peak in harmful algal bloom activity from July to October. This improved early seasonal prediction can provide timely information to policymakers for adopting proper planning and mitigation strategies such as restrictions in harvesting and help in monitoring toxins in shellfish to keep contaminated products off the market.
Thick and old sea ice in the Beaufort Sea during summer 2020/21 was associated with enhanced transport
Authors: Moore G.W.K. , Steele Michael , Schweiger Axel J. , Zhang Jinlun , Laidre Kristin L. 
Published: August 2022

AbstractThe Arctic Ocean has seen a remarkable reduction in sea ice coverage, thickness and age since the 1980s. These changes are most pronounced in the Beaufort Sea, with a transition around 2007 from a regime dominated by multi-year sea ice to one with large expanses of open water during the summer. Using satellite-based observations of sea ice, an atmospheric reanalysis and a coupled ice-ocean model, we show that during the summers of 2020 and 2021, the Beaufort Sea hosted anomalously large concentrations of thick and old ice. We show that ice advection contributed to these anomalies, with 2020 dominated by eastward transport from the Chukchi Sea, and 2021 dominated by transport from the Last Ice Area to the north of Canada and Greenland. Since 2007, cool season (fall, winter, and spring) ice volume transport into the Beaufort Sea accounts for ~45% of the variability in early summer ice volume—a threefold increase from that associated with conditions prior to 2007. This variability is likely to impact marine infrastructure and ecosystems.
The complex genetic basis of fibromuscular dysplasia, a systemic arteriopathy associated with multiple forms of cardiovascular disease
Authors: Georges Adrien , Bouatia-Naji Nabila 
Published: August 2022

Abstract
Artery stenosis is a common cause of hypertension and stroke and can be due to atherosclerosis accumulation in the majority of cases and in a small fraction of patients to arterial fibromuscular dysplasia (FMD). Artery stenosis due to atherosclerosis is widely studied with known risk factors (e.g. increasing age, male gender, and dyslipidemia) to influence its etiology, including genetic factors. However, the causes of noninflammatory and nonatherosclerotic stenosis in FMD are less understood. FMD occurs predominantly in early middle-age women, a fraction of the population where cardiovascular risk is different and understudied. FMD arteriopathies are often diagnosed in the context of hypertension and stroke and co-occur mainly with spontaneous coronary artery dissection, an atypical cause of acute myocardial infarction. In this review, we provide a comprehensive overview of the recent advances in the understanding of molecular origins of FMD. Data were obtained from genetic studies using complementary methodological approaches applied to familial, syndromic, and sporadic forms of this intriguing arteriopathy. Rare variation analyses point toward mechanisms related to impaired prostacyclin signaling and defaults in fibrillar collagens. The study of common variation, mainly through a recent genome-wide association study, describes a shared genetic link with blood pressure, in addition to point at potential risk genes involved in actin cytoskeleton and intracellular calcium homeostasis supporting impaired vascular contraction as a key mechanism. We conclude this review with future strategies and approaches needed to fully understand the genetic and molecular mechanisms related to FMD.
Estimating Early Language Input in Deaf and Hard of Hearing Children With the Language Access Profile Tool
Authors: Hall Matthew L. , De Anda Stephanie 
Published: August 2022

Purpose:
The purpose of this study was to describe the Language Access Profile Tool (LAPT) and its psychometric properties with the aim of evaluating its suitability as an alternative to the deaf or hard of hearing (DHH) Language Exposure Assessment Tool (D-LEAT) in clinical practice with DHH children age 12 years and younger.

Method:
We administered both the LAPT and D-LEAT to the caregivers of 105 DHH children 12 years old and younger from across the United States, 40% of whom were interviewed again after a delay of at least 1 month. Each interview resulted in a child-specific estimate of their cumulative experience with language input, expressed as a proportion divided across eight categories.

Results:
Participants in the sample reported experience with all eight input categories, but four categories were common and four were rare. Estimates for all input categories were consistent at both initial and follow-up interviews. Estimates for each input category were also strongly correlated with the corresponding estimates from the D-LEAT, although correlations for the rare categories should be interpreted cautiously.

Conclusions:
The LAPT demonstrates sufficient test–retest reliability and convergent validity to be a useful and more user-friendly alternative to the D-LEAT. We provide recommendations for how the LAPT and the D-LEAT can be best used in their current form.

Supplemental Material:

https://doi.org/10.23641/asha.20669001
Which Videofluoroscopy Parameters Are Susceptible to the Influence of Differences in Barium Product and Concentration?
Authors: Steele Catriona M. , Barrett Emily , Peladeau-Pigeon Melanie 
Published: August 2022

Purpose:
Prior studies suggest there may be differences in videofluoroscopic measures of swallowing across different barium concentrations. Whether different barium products of identical concentration result in similar swallowing physiology remains unknown. This is important, as barium intended for videofluoroscopy (i.e., Bracco Varibar) is not available globally. Our aim was to identify differences in healthy swallowing across five different barium stimuli.

Method:
Twenty healthy adults (10 women), aged 22–54 years, underwent videofluoroscopy including comfortable sips of thin liquid barium: two sips of 20% weight-to-volume (w/v) barium prepared with E-Z-HD powder, and two sips each of 20%w/v and 40%w/v barium prepared with Liquid Polibar Plus and E-Z-Paque powder. Recordings were analyzed according to the Analysis of Swallowing Physiology: Events, Kinematics and Timing Method. Measures of timing, kinematics and residue were obtained. Chi-square, Friedman's, and Wilcoxon signed-ranks test were used to identify differences across stimuli.

Results:
Significant differences were seen across barium stimuli for upper esophageal sphincter (UES) opening duration, UES diameter, pharyngeal area at maximum constriction, and residue. In all cases, smaller values were seen with the 20%w/v E-Z-HD stimulus; however, this stimulus had questionable opacity for visualization. Patterns of residue severity were not explained by barium concentration.

Conclusions:
This study confirms that some measures of swallowing are influenced by barium product and/or concentration. Measures are not necessarily similar across different barium products at the same concentration. This study illustrates the importance of using standard and appropriate stimuli in videofluoroscopy, and for clinicians to report not only the product but also the concentration of stimuli used.

Supplemental Material:

https://doi.org/10.23641/asha.20669712