bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Relationship Between Dental Caries, Food Intake and Body Composition in School-Age Children
Authors: Özel İrem Çağla , Ayhan Nurcan Yabancı , Ünverdi Gizem Erbaş 
Published: May 2022

Abstract
Purpose: The aim of this study is to evaluate the relationship between dental caries, food intake and body composition in school-age children. Methods: The study was conducted on 210 children, 105 boys and 105 girls, aged 6-12 years, without mental and / or physical disorders. Oral examinations of the children were performed by pediatric dentists and DMFT –DMFS and dmft-dmfs values were recorded. A detailed food frequency questionnaire was applied to determine consumption frequency of dairy products and sugar‐added foods. Children's height, waist and hip circumference measurements were taken by the researcher; body weight and body composition were evaluated with Tanita BC-601F body analyzer. Results: DMFT values were found to be lower in children who consumed milk, buttermilk and white cheese daily, but this differences were not statistically significant (p>0.05).Body weight and waist circumference are found to be positively correlated with DMFT and negatively correlated with dmft. BMI was found to be positively correlated with DMFT; negatively correlated with dmft (p<0.05).Conclusions: In this study, dental caries was found to be associated with food consumption and body mass index. In children, consumption of sugary foods should be reduced and consumption of milk and dairy products should be increased.
Infective Endocarditis in Childhood: a Single- Center Experience of 26 Years
Authors: DEMIR Fadli , VARAN Celal , ERDEM Sevcan , ATMIŞ Anıl , AKBAŞ Tolga , SUBAŞI Berivan , GÜZEL Yasin , ÖZBARLAS Nazan 
Published: May 2022

Abstract
Background: Despite developments in diagnosis and treatment, infective endocarditis (IE) continues to be a disease with high rates of morbidity and mortality. The aim of this study was to present the clinical and microbiological characteristics of patients with IE. Methods: A retrospective evaluation was made of patients diagnosed with IE in our clinic between 1995 and 2021. The clinical and laboratory characteristics of the patients were recorded together with conditions constituting a risk for the development of IE, treatment, and surgical outcomes. Results: Evaluation was made of 68 patients with a mean age of 7.3 years (range, 3 months-17 years), diagnosed with IE. An underlying cause of congenital heart disease was determined in 47 (69%) patients, and rheumatic valve disease in 3 (4.4%). There was no structural heart disease in 18 (26%) patients. A causative organism was found in 41 (60%) cases, and the micro-organism most often determined was viridans group streptococcus. No difference was determined between the patients diagnosed before and after 2007 in respect of the frequency of viridans streptococcus (p>0.05). Septic emboli were seen in 18 (26%) patients, of which 17 required surgical treatment. In 5 of 11 (16.6%) patients with mortality, the agent was S. aureus. Of the total IE patients, 28 (41%) were evaluated as hospital-acquired IE. The most frequently determined agents in this group were staphylococcus epidermidis (n:6) and S. aureus (n:5). Conclusion: Although congenital heart diseases continue to be the greatest risk factor for IE, there is an increasing frequency of IE in patients with no structural heart disease. Mortality rates are still high in IE, especially in S. aureus endocarditis.
EGFR mutant status and tyrosine-kinase inhibitors affect the GKRS outcomes for NSCLC brain metastases
Authors: Liao Hung-Ruei , Chiang Chi-Lu , Shen Chia-I , Chen Ching-Jen , Yang Huai-Che , Wu Hsiu-Mei , Luo Yung-Hung , Hu Yong-Sin , Lin Chung-Jung , Chung Wen-Yuh , Shiau Cheng-Ying , Guo Wan-Yuo , Pan David Hung-Chi , Lee Cheng-Chia 
Published: May 2022

Abstract
Objective: Tyrosine kinase inhibitors (TKIs) is the first-line treatment for EGFR-positive non-small cell lung cancer (NSCLC); however, its applicability to patients with wild-type NSCLC remains an issue of contention. This study compared the effects of gamma knife radiosurgery (GKRS) alone versus combining GKRS and TKIs in treating two genetic forms of NSCLC.Methods: This retrospective study examined 479 NSCLC patients with 1982 brain metastases who underwent GKRS and for whom imaging follow-up data or death records were available. All our patients were consecutive. All gene mutations were confirmed by lung biopsy. The three main endpoints in this study were overall survival (OS), local intracranial tumor control (LC) , and distal intracranial tumor control (DC).Results: There were 296 NSCLC patients with EGFR positive: TKI treatment (n= 262) and without TKI treatment (n= 34). GKRS + TKIs was more effective than GKRS alone in terms of OS (HR: 0.53, p= 0.085) and DC (HR: 0.51, p<0.001). There were 150 NSCLC patients with wild-type EGFR: TKI treatment (n= 50) and without TKI treatment (n= 100). GKRS + TKIs was less effective than GKRS alone in terms of OS (HR: 1.82, p= 0.049) and DC (HR: 1.40, p=0.011). We observed no difference in terms of LC in both genetic groups.Conclusions: Combining GKRS with TKIs proved effective in EGFR positive NSCLC patients; however, we do not observe the similar results when combining GKRS with TKIs for patients with wild-type NSCLC
Genetics of Kallmann syndrome: Single-center Experience with Review of NGS based Genetic Studies
Authors: Patil Virendra A , Lila Anurag Ranjan , Shah Nalini , Arya Sneha , Ekbote Alka V , Sarathi Vijaya , Shah Ravikumar , Jadhav Swati S , Memon Saba Samad , Karlekar Manjiri , Bandgar Tushar 
Published: May 2022

Abstract
Purpose: To describe phenotype-genotype data of Asian-Indian Kallmann syndrome (KS) from our center and perform a systematic review of genetic studies using next-generation sequencing (NGS) in KS.Methods: Seventy-eight KS probands from our center and 398 probands from published studies were included. Per-patient genetic variants were analyzed as per ACMG-guidelines. Molecular diagnosis was defined as the presence of pathogenic or likely pathogenic variant(s) in known CHH gene/s following zygosity status as per the known mode of genetic inheritance.Result: Molecular diagnosis at our center was observed in 20.5% probands (ANOS1:10.2%, FGFR1: 6.4%, PROKR2: 2.5%, and PROK2, SOX10, FGF8, GNRHR: 1.3% each). Molecular diagnosis was reached more often in patients with severe than partial reproductive phenotype (28.3% vs. 4%, p=0.0013). Our center adds eight novel variants. In a per-patient systematic review (including our cohort), the molecular diagnosis was reached in 30.8%, ranging from 16.6-72.2% at different centers. The affected genes were FGFR1 (9.6%), ANOS1 (7.7%), PROKR2 (6.5%), CHD7 (4.6%), oligogenic (1.9%), FGF8 (1%), SOX10 (1%), and others (PROK2, SEMA3A, IL17RD, GNRHR:<1% each). FGFR1 was the most commonly affected gene in most cohorts except Asia and Brazil, where PROKR2 (in China and Japan) and ANOS1 (in India and Brazil) were the commonest.Conclusion(s): The global molecular diagnosis rate was 30.8% in KS cohorts whereas that in our cohort was 20.5% with a higher rate (28.3%) in those with severe reproductive phenotype. The most commonly affected gene in KS patients was FGFR1 globally, PROKR2 in East Asia, and ANOS1 in India and Brazil.
Comprehensive relationship analysis of the Long Non-Coding RNAs (lncRNAs) and the target mRNAs in Response to the infection of Edwardsiella anguillarum in European eel (Anguilla Anguilla) inoculated with Freund's adjuvant
Authors: Wu Liqun , Yin Zhijie , Zheng Zhijin , Tang Yijun , Guo Songlin 
Published: May 2022

Abstract
Freund's complete (FCA) and incomplete adjuvants (FIA), generally applied in subunit fishery vaccine, have not been explored on the molecular mechanism of the nonspecific immune enhancement. As long noncoding RNAs (lncRNAs) play vital regulating roles in various biological activities, in this study, we examined the genome-wide expression of transcripts in the liver of European eel (Anguilla anguilla, Aa) inoculated with FCA and FIA (FCIA) to elucidate the regulators of lncRNAs in the process of Edwardsiella anguillarum (Ea) infection and Aa anti-Ea infection using strand specific RNA-seq. After eels were challenged by Ea at 28 d post the first inoculation (dpi), compared to the control uninfected eels (Li group), the control infected eels (Con_Li group) showed severe bleeding, hepatocyte atrophy and thrombi formed in the hepatic vessels of the liver, although eels inoculated with FCIA (FCIA_Li group) also formed slight thrombi in the hepatic vessels. Compared to the FCIA_Li group, there was about 10 times colony forming unit (cfu) in the Con_Li group per 100 µg liver tissue, and the relative percent survival (RPS) of eels was 50% in FCIA_Li vs Con_Li. Using high-throughput transcriptomics, differential expressed genes (DEGs) and transcripts were identified and the results were verified using fluorescence real-time polymerase chain reaction (qRT-PCR). Interactions between the differential expressed lncRNAs (DE-lncRNAs) and the target DEGs were explored using Cytoscape according to their co-expression and co-location relationship. We found 13499 lncRNAs (10176 annotated and 3423 novel lncRNAs) between 3 comparisons of Con_Li vs Li, FCIA_Li vs Li and FCIA_Li vs Con_Li, of which 111, 110 and 129 DE-lncRNAs were asertained. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of DEGs targeted by DE-lncRNAs revealed these DEGs mainly involved in single-organism cellular process in BP, membrane in CC and binding in MF, and KEGG pathways showed that the target DEGs in co-expression and co-location enriched in cell adhesion molecules. Finally, 118 DE-lncRNAs target 1161 DEGs were involved in an interaction network of 8474 co-expression and 333 co-location related links, of which 16 DE-lncRNAs play vital roles in anti-Ea infection. Taken together, the interaction networks revealed that DE-lncRNAs underlies the process of Ea infection and Aa anti-Ea infection.
Impact of central venous port implantation method and access choice on outcomes
Authors: Erdemir Ayhan , Rasa Hüseyin Kemal 
Published: May 2022

Abstract
Background: Although the number of patients who needs central venous ports for permanent vascular accesses is increasing we still don’t have a “gold standard” for implantation technique. In this retrospective study, considering the patient outcomes and adverse events we aimed to identify the method that should be favored.Methods: 200 patients divided into two groups according to the access method were retrospectively evaluated. Jugular vein access constitutes the first group and the patients that we used the subclavian vein were defined as the second. Groups were compared according to age, gender, side, diagnosis, follow-up period, chemotherapy agents administered, the number and severity of complications. Results: There was no statistically significant difference between the groups regarding age, gender, side, number of chemotherapy drugs and the duration of port usage (p > 0.05). Only two patients in Group 1 had complications whereas in Group 2 we observed nineteen complications (p < 0.05). No port occlusion was found in Group 1 but 4 patients’ catheters were occluded in Group 2 (p<0.05). Against one in Group 1 three ports were infected in Group 2 (p<0.05). Two port ruptures, two pneumothorax, one revision due to a mechanical problem, a tachyarrhythmia during implantation and four suture line problems were also recorded in Group 2 patients. Conclusion: Despite limitations our results robustly propounded that the jugular vein route is safer than subclavian vein approach for central venous port implantation and also it would be sufficient enough to evaluate ports once every 2 months.
Mining Association Rules for Classification Using Frequent Generator Itemsets in arules Package
Authors: Ledmi Makhlouf , Souidi Mohammed El Habib , Hahsler Michael , Kara-Mohamed Chafia , Ledmi Abdeldjalil 
Published: May 2022

Abstract
Mining frequent itemsets is an attractive research activity in data mining whose main aim is to provide useful relationships among data. Consequently, several open-source development platforms are continuously developed to facilitate the users' exploitation of new data mining tasks. Among these platforms, the R language is one of the most popular tools. In this paper, we defend the usefulness of providing a new version of arules package that can mine frequent generator itemsets, we describe the possibility of mining frequent generator itemsets in arules package, and we discuss what generators can be used for a classification task through an application example.
Bacillus cihuensis PVGOC-3 isolated from Guanabara Bay, Brazil, and its high potential for oil degradation
Authors: Ferreira Rachel de M. , Nascimento Rodrigo P. do , Stapelfeldt Danielle M. A. , Seldin Lucy , Moreira Maria de F. R. 
Published: May 2022

Abstract
The aim of the study was to isolate bacterial strains from an environment contaminated by hydrocarbons and investigate the degradation potential of petroleum derivatives with the use of used lubricating oil from a ship engine. Nine strains of possibly hydrocarbon-degrading bacteria were isolated from a sample of contaminated moist soil in the marginal rhizosphere at Guanabara Bay, located in the city of Rio de Janeiro, Brazil, and a triage for biosurfactant-producing bacteria was performed. Based on the results for degradation of used ship engine lubricating oil with dye 2.6 dichlorindophenol as a redox indicator, the PVGOC-3 scan was selected for growth and degradation tests. Amplification and sequencing of the rRNA 16S gene showed that the PVGOC-3 strain belongs to the species Bacillus cihuensis strain. The PVGOC-3 strain was able to degrade about 60.8% of the lubricating oil in 168 h at an initial concentration of 4.534 g L-1 according to the biodegradation analysis. According to the studies, Bacillus cihuensis presented a high potential in degrading used ship engine oil.
Tobacco Nicotine Promote TRAIL Resistance in Lung Cancer by SNHG5
Authors: Xiao Xin , Xu Juan , Sheng Xiaoan , Wang Chao , Dong Juanjuan , Shi Xianfeng 
Published: May 2022

Abstract
Tobacco nicotine can lead to carcinogenesis. It is a well-known risk factor for lung cancer. However, whether tobacco nicotine can induce drug resistance in lung cancer is incompletely defined. Our objective is to identify the tumor necrosis factor (TNF) related apoptosis inducing ligand (TRAIL) resistance of long noncoding RNAs (lncRNAs) which are differentially expressed in smokers and nonsmokers with lung cancer. The results suggested that small nucleolar RNA host gene 5 (SNHG5) was upregulated by nicotine inducing and markedly decreased the levels of cleaved caspase3. We found cytoplasm lncRNA SNHG5 overexpression correlated with TRAIL resistance in lung cancer and SNHG5 can interact with X-linked inhibitor of apoptosis protein (XIAP) to promote TRAIL resistance. Our findings suggest that nicotine promotes TRAIL resistance in lung cancer by SNHG5/XIAP.
Bioinformatics analysis identifies NDRG1 gene variants that may be relevant to thyroid cancer risk and prognosis
Authors: Borré Georgia Barcia , Pimenta Andressa Teles , Chmieleski Gabriela Souza , Moyses Gabriela Resende , Rabi Larissa Teodoro , Peres Karina Colombera , Teixeira Elisângela Souza , Bufalo Natassia Elena , Ward Laura Sterian 
Published: May 2022

Abstract
The study of genetic alterations that alter genes that regulate normal cellular processes, providing growth advantages and metastatic capabilities to tumor cells is fundamental for a deeper understanding of the molecular nature of different types of cancer, while revealing new therapeutic targets. The NDRG1 gene encodes a protein whose expression has been associated with tumor development and progression, but data on different types of cancer are still dubious and controversial. In order to establish a possible relationship of NDRG1 gene polymorphisms with susceptibility, clinical characteristics and evolution of patients with thyroid tumors, we undertook a comprehensive bioinformatics investigation using a series of in silico tools. We demonstrate that NDRG1 rs201348291 and rs151322132 SNPs are potential and important candidates that can influence the process of pathological development of neoplasms, including thyroid neoplasms. Further validation on thyroid nodule patients and functional studies may confirm their clinical utility in the management of these patients.
Prenatal ultrasound evaluation of fetal cutaneous hemangioma and related complications
Authors: Liao Minyan , He Bingjia , Xiao Zhen , Wang Li-min , Chen Yan , Liu Xiang-jiao , Shu Shuang , Shang Ning 
Published: May 2022

Abstract
Objectives：Congenital hemangiomas are rare benign vascular tumors but can lead to serious adverse pregnancy outcomes. We explored the clinical applications of prenatal ultrasound for evaluating fetal cutaneous hemangioma and associated complications.Methods: A retrospective observational study was conducted comprising a population of pregnant women with fetal cutaneous hemangioma, the latter diagnosed by prenatal ultrasound, between January 2016 and December 2020. The clinical characteristics, sonographic images, complications, and pregnancy outcomes were documented and analyzed.Results: We identified 20 cases of fetal cutaneous hemangioma diagnosed by the prenatal ultrasound and confirmed by the postpartum examinations. Most hemangiomas were in the head and neck (55%), with either solid isoechoicity (25%) or solid mildly hyperechoicity (25%) and clear boundary (80%). Eight (40%) fetuses were found to have complications, which often occurred in fetuses with large hemangiomas (67% with maximum diameter ≥5 cm; 100% with a volume ≥40 cm3). The most common complications were cardiac-related (88%), including elevated cardiothoracic area ratio, atrioventricular valve regurgitation, and fetal edema. A large hemangioma was usually associated with advanced gestational age and a fast hemangioma growth rate. In five (25%) cases, the pregnancy was terminated; these involved hemangioma of the head or neck. One newborn developed Kasabach-Merritt phenomenon, pulmonary hemorrhage, respiratory distress, and died 3 days after birth. Among the 14 (70%) fetuses that survived birth, all hemangiomas disappeared or regressed after treatments with propranolol, interventional surgery, or routine observations.Conclusions: Prenatal ultrasound examination could accurately diagnose fetal cutaneous hemangioma and related complications to facilitate appropriate management during the pregnancy.
Multi-view grading method for measuring tomato shape features using machine vision
Authors: Chen Liping , He Tingting , Li Zhiwei , Zheng Wengang , An Shunwei , Zhong Lili Zhang 
Published: May 2022

Abstract
Owing to the requirements of a high yield and high-quality tomatoes, tomato grading is important—particularly for fruit morphology. Machine vision provides a fast and nondestructive manner to address this demand, and accuracy has become the focus of attention. In this study, the gamma correction method was used for preprocessing to enhance the surface reflection of tomatoes, and Otsu’s method was used to segment the A scalar diagram under the LAB color model. On this basis, two levels of exploration were conducted. First, new evaluation indices were proposed for different views. For the top view, two shape-evaluation indices were established: the area ratio of the maximum inscribed circle to the maximum circumscribed circle and the dispersion of the contour centroid distance (extreme value and coefficient of variation). For the side view, the difference between the maximum and minimum centroid distances in the contour was established as a shape index. Compared to with commonly used shape features, the shape-detection accuracy of the proposed indicators proposed was > 7% higher. Second, an evaluation method based on multi-view fusion was developed by combining the advantage indices for different views. The detection accuracy was 96%, which was 9.40–9.70% higher than those for the top and side views alone. The proposed evaluation method combining top views (dispersion of centroid distance) with side views (difference between maximum and minimum centroid distances) is effective for classifying tomatoes.
Effects of cold stress on hormone contents, immune responses and HSL and UCP1 expression in the fat tissue of Altay and Hu lambs
Authors: Zhang Li , Gao Jingwen , Wang Xiaoxuan , Wei Dianhua , Zen Zongbo , Qi Yayin 
Published: May 2022

Abstract
The aim of the present study was to explore the effects of cold stress at different levels on hormone contents, immune function, and heat-producing genes expression in Altay and Hu lambs. The lambs were randomly divided into three experimental groups maintained under different temperature conditions (G2: -20±3℃ and G3: -30±3℃) for acute cold stress (6 h, 24 h) and chronic cold stress (4 d, 12 d, 24 d) and one thermoneutral group was maintained at the control temperature (G1:15±2℃). The levels of leptin in the serum of two breeds was lower in the cold stress group than in G1 (P<0.01), and the norepinephrine levels was higher in the cold stress groups than in the thermoneutral group (P<0.01). The levels of epinephrine in the serum of Altay and Hu lambs dynamically fluctuated in the G2 and G3. The glucagon levels of the Hu lambs were significantly higher in G2 and G3 than in G1 (P<0.01). The glucagon levels of Altay lambs were significantly decreased in G2 compared with G1 (P<0.01). The insulin levels in the serum of the Altay lambs were significantly lower in the stress group than in G1 (P<0.01). The insulin levels in the serum of Hu lambs tended to irregularly fluctuation in the experimental groups compared with the G1. Histopathological examination showed that the intestinal tissues of the cold stress group were seriously injured. The mRNA expression levels of IL-2, IL-4, IL-10, IL-12 and IFN-β in the spleen varied at different time periods after cold stress, but were overall higher in the cold stress group than in G1 (P<0.01). After cold stimulation, the expression of uncoupling protein 1 and hormone-sensitive lipase in fat tissues showed dynamic changes, but were increased significantly compared with control group (P<0.01). These results suggested that cold stress induced the endocrine system and influenced the immune function of the spleen. The increased expression of HSL and UCP1 may play a role in energy metabolism in the response of animals to cold stress. This work add an important data for the studding the ability of animal to adapt with the colder regions.
Role of Vascular endothelial growth factor in radiotherapy resistance to Esophageal squamous cell carcinoma
Authors: Li Xin , Su Xinyu , Yan Chen , Ma Yuanyuan , Li Heng , Xia Jianhong , Li Hongliang , Jiang Qian , Zhou Liqing , Zou Zhengyun 
Published: May 2022

Abstract
Vascular endothelial growth factor(VEGF) is related to the radiation resistance of tumors, resulting in the failure of tumor radiotherapy. The purpose of this study was to discuss the role of VEGF in radiotherapy resistance of esophageal squamous cell carcinoma(ESCC). We used the VEGF kit by ELISA to detect the serum VEGF level of ESCC patients who only received radiotherapy. The expression of VEGF in ESCC cells after siRNA treatment was verified by Western blot. The sensitivity of ESCC cells to radiation after knocking down VEGF was analyzed by Clonogenic assay and Cell counting kit(CCK-8). The results showed that the level of serum VEGF in patients with ESCC before and after radiotherapy was related to the clinical response, and it was confirmed that knocking down the expression of VEGF in ESCC cells improved the sensitivity to radiation.