bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of health systems for health security: a scoping review revealing the need for improved conceptual and practical linkages
Authors: Brown Garrett Wallace , Bridge Gemma , Martini Jessica , Um Jimyong , Williams Owain D. , Choupe Luc Bertrand Tsachoua , Rhodes Natalie , Ho Zheng Jie Marc , Chungong Stella , Kandel Nirmal 
Published: May 2022

AbstractBackgroundPractical links between health systems and health security are historically prevalent, but the conceptual links between these fields remain under explored, with little on health system strengthening. The need to address this gap gains relevance in light of the COVID-19 pandemic as it demonstrated a crucial relationship between health system capacities and effective health security response. Acknowledging the importance of developing stronger and more resilient health systems globally for health emergency preparedness, the WHO developed a Health Systems for Health Security framework that aims to promote a common understanding of what health systems for health security entails whilst identifying key capacities required.Methods/ resultsTo further explore and analyse the conceptual and practical links between health systems and health security within the peer reviewed literature, a rapid scoping review was carried out to provide an overview of the type, extent and quantity of research available. Studies were included if they had been peer-reviewed and were published in English (seven databases 2000 to 2020). 343 articles were identified, of those 204 discussed health systems and health security (high and medium relevance), 101 discussed just health systems and 47 discussed only health security (low relevance). Within the high and medium relevance articles, several concepts emerged, including the prioritization of health security over health systems, the tendency to treat health security as exceptionalism focusing on acute health emergencies, and a conceptualisation of security as ‘state security’ not ‘human security’ or population health.ConclusionExamples of literature exploring links between health systems and health security are provided. We also present recommendations for further research, offering several investments and/or programmes that could reliably lead to maximal gains from both a health system and a health security perspective, and why these should be explored further. This paper could help researchers and funders when deciding upon the scope, nature and design of future research in this area. Additionally, the paper legitimises the necessity of the Health Systems for Health Security framework, with the findings of this paper providing useful insights and evidentiary examples for effective implementation of the framework.
Influence of the sociodemographic profile of hunters on the knowledge and use of faunistic resources
Authors: Souza Jeferson de M. , Lins Neto Ernani M. F. , Ferreira Felipe S. 
Published: May 2022

AbstractBackgroundHunting wild animals is essential for nutrition, clothing, predator control and disease treatment. As part of a system based on food choices and uses, it is influenced by ecological, economic and sociocultural patterns. In this context, the aim is to identify the game fauna of interest in the Brazilian semiarid region; indicate the methods, uses, patterns of choices and cultural importance of the fauna and identify which sociodemographic variables influence the knowledge and use of faunal resources.MethodsInformation on hunting and fauna use was obtained through semi-structured interviews, complemented with free interviews and informal conversations. The cultural importance of the species was calculated through the current use value. The generalized linear model was created to verify whether the sociodemographic profile of hunters influences the knowledge and use of game species.ResultsThe results showed a representativeness of 56 species. The group of birds was the most representative in terms of taxonomic richness (48.2%), followed by the group of mammals (26.8%), reptiles (21.4%) and amphibians (3.6%). The animals mentioned are used for food, trade, control hunting (slaughter of animals considered invaders of property or harmful to humans), pets, zootherapy and ornamentation. Sociodemographic variables shaped the knowledge of faunal resources, in which the age of hunters showed a negative correlation with the number of known species.ConclusionsThe meaning and forms of use attributed to each species depend on ecological, economic and sociocultural factors, which dictate the relationship between human communities and natural resources. Socioeconomic variables shape hunting patterns in all its aspects, whether in perception that hunters have of the resources, forms of use and utilization of hunting strategies.
A review of the disruption of breastfeeding supports in response to the COVID-19 pandemic in five Western countries and applications for clinical practice
Authors: Turner Sarah , McGann Bridget , Brockway Meredith ’Merilee’ 
Published: May 2022

AbstractBackgroundThe COVID-19 pandemic has significantly altered how breastfeeding support is provided, resulting in mixed breastfeeding outcomes and experiences for mothers. The World Health Organization has consistently supported breastfeeding from the beginning of the pandemic. However, recommendations from obstetrical and gynaecological societies within individual countries have varied in their alignment with this guidance, resulting in inconsistent recommendations. It is unknown how breastfeeding guidelines, maternal breastfeeding experiences, and breastfeeding initiation and duration compared across five Western countries. The current study is comprised of two parts, each with a different objective. Part One objective: to review pandemic-related changes in professional society guidelines on breastfeeding support in Australia, New Zealand, Canada, the United Kingdom, and the United States; and Part Two objective: to conduct a narrative review to summarize the evidence of how the pandemic has changed breastfeeding initiation, duration, and mothers’ breastfeeding experiences during the pandemic in these five countries and provide recommendations for clinical lactation support.MethodsWe searched for indicators that are impactful on breastfeeding outcomes: skin-to-skin contact, rooming in, direct breastfeeding and breast washing, in the five countries mentioned above and compared these to the recommendations from the World Health Organization. Next, we conducted a narrative review of the literature from these five countries to explore how the pandemic altered breastfeeding outcomes and used this information to provide suggestions for clinical practice moving forward.ResultsRecommendations on the four practices above differed by country and were not always in alignment with the World Health Organization recommendations. Mother-infant separation after birth in the United States was associated with a lower prevalence of breastfeeding initiation and duration. While some mothers reported positive breastfeeding experiences during the pandemic, many mothers indicated negative experiences related to decreased social and professional support.ConclusionsThe pandemic can inform practice recommendations and can be viewed as an opportunity to permanently modify existing methods to support breastfeeding families. The use of virtual care increased during the pandemic and should continue with specific considerations for prioritizing in-person care. This will help to provide more timely and accessible support for breastfeeding mothers.
Radiological changes in shoulder osteoarthritis and pain sensation correlate with patients’ age
Authors: Märtens Nicole , März Vincent , Bertrand Jessica , Lohmann Christoph H. , Berth Alexander 
Published: May 2022

AbstractPurposeOsteoarthritis (OA) is one of the most common musculoskeletal disorders in the aging population. The correlation of radiographic OA severity, disability and pain is variable and inconsistent for the different joints. This study aims to elucidate the relationship between histological and radiological signs of shoulder OA with pain sensation and functional impairment to potentially adapt the recommendation for surgical treatment for primary total shoulder arthroplasty (TSA).MethodsForty-four patients with shoulder OA undergoing TSA using an anatomic stemless implant were included in this study. The radiological OA severity was scored pre-operatively on true ap X-rays according to the Kellgren–Lawrence score (KL-Score). Acromial types according to Bigliani were defined by pre-operative radiological images. The histological OA severity was determined according to the OARSI-Score using bone–cartilage sections from loaded areas of the humeral head. Pain was quantified using the visual analog scale (VAS). The functional status was assessed by the items “mobility” and “strength” out of the Constant–Murley score (CS Score). Demographic data including BMI, age, gender, diabetes mellitus and smoking were recorded.ResultsThere was no correlation between radiographic and histological severity in shoulder OA. However, a correlation of age and the severity of radiological changes was observed. Further, pain did not correlate with histological or radiological scores, whereas it correlated with age and the presence of diabetes mellitus. The functional shoulder status (mobility, strength) correlated with the severity of radiological changes, but not with the histologic scoring, which correlated with nicotine abuse.ConclusionThis study shows that increased age is the main determinant of radiological changes in shoulder OA, as well as pain. Therefore, age and pain sensation should be considered as important parameters for the recommendation for TSA.
Does minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) influence functional outcomes and spinopelvic parameters in isthmic spondylolisthesis?
Authors: Ali Elsayed Mohamed Selim , El-Hewala Tarek Abdelsamad , Eladawy Amr Mohamed , Sheta Reda Ali 
Published: May 2022

Abstract
Purpose
We assessed the efficacy of minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) in patients with low-grade isthmic spondylolisthesis.

Methods
We included 24 symptomatic patients who underwent MIS-TLIF between December 2017 and December 2020. Patients were followed up clinically by the Oswestry Disability Index (ODI) and Visual Analogue Scale (VAS) for back and VAS for leg pain, as well as radiological radiographs after 6 weeks, 6 months, and at final follow-up (at least 12 months). Measured parameters included C7 sagittal vertical axis (SVA), pelvic tilt (PT), pelvic incidence (PI), sacral slope (SS), Meyerding slip grades, lumbar lordosis (LL), L1–L4 angle, L4–S1 angle, and segmental lordosis (SL) of the affected segment. The mismatch between the PI and LL was also measured.

Results
VAS for back, VAS for leg pain, and ODI significantly improved postoperatively (all p < 0.001). We observed significantly decreased mean values of PT and slip percentage and increased mean values of SS and LL (all p < 0.05). We observed a significant reduction in L1–L4 lordosis and a significant increase in L4–S1 lordosis. The final PT, SS, and LL (total and L1–L4) were significantly higher in group III patients (n = 15) than the values of group II patients (n = 9). None of the patients became unbalanced postoperative, and all patients had a normal matching between the PI and the LL postoperatively.

Conclusions
MIS-TLIF is a safe procedure for managing low-grade isthmic spondylolisthesis with significant improvement in clinical and radiological outcomes. It can correct and maintain a proper spinopelvic alignment.
Medialization of trochlear groove was correlated with extended lateral trochlear in trochlear dysplasia: a transverse CT analysis
Authors: Dong Conglei , Zhao Chao , Kong Lingce , Piao Kang , Hao Kuo , Wang Fei 
Published: May 2022

Abstract
Purpose
To investigate the difference of trochlear width between normal and dysplastic trochlear and to analyze whether the medialization of trochlear groove was correlated with abnormal width of trochlear facets in trochlear dysplasia.

Methods
This study involved CT scans of fifty knees with trochlear dysplasia (TD group) and fifty knees without obvious trochlear dysplasia (Normal group). The linear distance from the medial femoral epicondyle to the various reference points was measured on axial CT images which included the medial edge of medial trochlear facet (dMTE), trochlear groove (dTG), and the lateral edge of lateral trochlear facets (dLTE). The medial and lateral trochlear width was calculated and standardized by the width of the anatomical epicondylar axis. Pearson’s correlation analysis was performed between the dTG and the width of the medial and lateral trochlear.

Results
The reliability of the results was good. Intraclass correlation coefficient (ICC) ranged from 0.89 to 0.97. The dMTE was significantly greater in the TD group than the normal group (32.7 ± 5.1% vs. 29.6 ± 3.5%, p = 0.009). There was no significant difference in the dLTE between groups. The dTG was reduced in the TD group compared with the normal group (45.2 ± 4.1% vs. 49.1 ± 3.9%, p = 0.019). In the TD group, there was a significant reduction in the medial trochlear width (13.9 ± 4.1% vs. 19.4 ± 2.9%, p < 0.001) and a significant increase in the lateral trochlear width (31.3 ± 4.0% vs. 26.9 ± 3.6%, p < 0.001) compared with the normal group. The dTG was significantly correlated with the lateral trochlear width (r value = − 0.693, p < 0.001) and not correlated with the medial trochlear width (r value = 0.044, p = 0.766) in trochlear dysplasia.

Conclusions
This study demonstrated that dysplasia of trochlear morphology was related to the reduction of medial trochlear width and increase in lateral trochlear width. The medialization of trochlear groove was significantly correlated with the increased lateral trochlear width.
Cytotoxicity of tranexamic acid to tendon and bone in vitro: Is there a safe dosage?
Authors: Bolam Scott M. , O’Regan-Brown Arama , Konar Subhajit , Callon Karen E. , Coleman Brendan , Dalbeth Nicola , Monk A. Paul , Musson David S. , Cornish Jillian , Munro Jacob T. 
Published: May 2022

Abstract
Introduction
Tranexamic acid (TXA) has been shown to be effective at reducing peri-operative blood loss and haemarthrosis in arthroplasty and arthroscopic soft tissue reconstructions. Intra-articular application, as an injection or peri-articular wash, is becoming increasingly common. Recent studies have shown TXA has the potential to be cytotoxic to cartilage, but its effects on human tendon and bone remain poorly understood. The aim of this study was to investigate whether TXA has any detrimental effects on tendon-derived cells and osteoblast-like cells and determine whether there is a safe dosage for clinical application.

Materials and methods
Primary tendon-derived cells and osteoblast-like cells were harvested from hamstring tendons and trabecular bone explants, respectively, and analysed in vitro with a range of TXA concentrations (0 to 100 mg/ml) at time points: 3 and 24 h. The in vitro toxic effect of TXA was investigated using viability assays (alamarBlue), functional assays (collagen deposition), fluorescent microscopy and live/apoptosis/necrosis staining for cell death mechanisms in 2D monolayer and 3D collagen gel cell culture.

Results
There was a significant (P < 0.05) decrease in tendon-derived cell and osteoblast-like cell numbers following treatment with TXA ≥ 50 mg/ml after 3 h and ≥ 20 mg/ml after 24 h. In tendon-derived cells, increasing concentrations > 35 mg/ml resulted in significantly (P < 0.05) reduced collagen deposition. Fluorescence imaging confirmed atypical cellular morphologies with increasing TXA concentrations and reduced cell numbers. The mechanism of cell death was demonstrated to be occurring through apoptosis.

Conclusions
Topical TXA treatment demonstrated dose- and time-dependent cytotoxicity to tendon-derived cells and osteoblast-like cells with concentrations 20 mg/ml and above in isolated 2D and 3D in vitro culture. On the basis of these findings, concentrations of less than 20 mg/ml are expected to be safe. Orthopaedic surgeons should show caution when considering topical TXA treatments, particularly in soft tissue and un-cemented arthroplasty procedures.
Virchow node of an unusual aetiology – the experience of a clinical case
Authors: Borges-Canha Marta , Sérgio-Neves João , Albuquerque Inês , Pacheco João , Silva Maria Manuel , Oliveira Ana Isabel , Carvalho Davide 
Published: May 2022

Abstract
Background
The cause of adult adenomegalies may be defiant. On the other hand, ectopic thyroid is a rare condition that happens in every 1:100000 to 300,000 of healthy individuals. Here, we present a case report that joins these two clinical rare and defiant challenges.

Clinical case
Forty-seven-year-old woman, with known thyroid nodules for several years. She had no other relevant personal or familiar history. At our appointment she had no complaints. At the physical examination she had a palpable right thyroid nodule (previously known). The routine blood analysis showed normal thyroid function. The routine cervical ultrasonography showed no dimensional progression of the known thyroid nodules and identified a 31x18mm nodule at the left supraclavicular fossa. The patient underwent a cervical, thoracic, and abdominal computed tomography that exhibited no relevant findings, such as abdominal malignancies. The cytology of the nodule showed characteristics that were “compatible with a benign follicular nodule in ectopic thyroid tissue”.

Conclusion
This is a rare case in which we incidentally found a follicular nodule in ectopic thyroid tissue in the left supraclavicular fossa. Given the rarity of the situation, clinical sense is the mainstay of treatment and follow-up.
Strongyloides stercoralis infection in dogs in Austria: two case reports
Authors: Unterköfler Maria Sophia , Eipeldauer Iris , Merz Sophie , Pantchev Nikola , Hermann Josef , Brunthaler René , Basso Walter , Hinney Barbara 
Published: May 2022

Abstract
Background
Strongyloides stercoralis is endemic in tropical and subtropical regions, but reports of infections in central and northern Europe have been recently increasing. Infections occur mainly in humans and dogs. In dogs, both dog-adapted and zoonotic S. stercoralis genotypes seem to occur. Clinical manifestations mainly include gastrointestinal and respiratory signs. The severity of the disease can vary greatly and depends on the immune status of the host. The infection is potentially fatal in immunosuppressed individuals, either medically induced or due to an underlying disease, in which hyperinfections and disseminated infections with extraintestinal parasite dissemination may occur.

Methods
Diagnosis was based on coproscopy, including flotation and the Baermann funnel technique, histology of small intestinal biopsies and molecular analysis of mitochondrial cytochrome oxidase subunit I (COI) and hypervariable regions I and IV (HVR I and HVR IV) of the nuclear 18S rDNA loci.

Results
Two independent cases of severe canine S.stercoralis infection in Austria are presented. In both cases, S. stercoralis was detected in histological sections of the small intestine and with the Baermann funnel technique. Molecular analysis revealed strains with zoonotic potential. Case 1 was a 1-year-old female French bulldog with a long history of respiratory and gastrointestinal signs, severe emaciation and apathy before S.stercoralis infection was diagnosed. Treatment with moxidectin (2.5 mg/kg body weight [BW], oral route) did not eliminate the infection, but treatment with ivermectin (0.2 mg/kg BW, subcutaneously) was successful. Case 2 consisted of two 2-month-old Pomeranian puppies, one female and one male, from a litter of four, which died soon after presenting dyspnoea and haemorrhagic diarrhoea (female) or torticollis (male); S.stercoralis infection was first diagnosed post-mortem.

Conclusion
More attention should be paid to this nematode because although it appears to be rare in Austria, it is easily overlooked on standard coproscopy unless a Baermann funnel technique is used, and even then, it can be missed. Moxidectin is not always successful in eliminating the infection, and treatment with ivermectin should be considered in cases of infection.

Graphical Abstract
Resident perception on the impact of point-of-care ultrasound in clinical care at a family medicine training program in Zambia
Authors: Haldeman Matthew S. , Kunka Evaristo , Makasa Mpundu , Birkland Bassim 
Published: May 2022

Abstract
Background
Patient access to necessary medical imaging in low- and middle-income countries (LMICs) remains a major obstacle, complicating clinician decision-making and compromising patient outcomes.

Methods
We implemented a longitudinal point-of-care ultrasound (POCUS) training program at a new Family Medicine residency in Zambia and subsequently evaluated residents’ perceptions on the impact of POCUS in patient care. Data were documented by the scanning resident via a post-scan survey, which assessed if/how the scan assisted in medical management, and if/how the scan changed that management. The primary endpoint was frequency of scans assisting and changing management. Data were summarized using descriptive statistics.

Results
Over the 1-year study period, 366 patient encounters occurred in which POCUS was utilized, resulting in a total of 542 unique POCUS scans. POCUS assisted in decision-making in 95.6% (350/366) of patient encounters, most commonly by helping to determine a diagnosis. POCUS changed management in 65.8% (235/357) of patient encounters, most commonly leading to a medication change.

Conclusions
Zambian resident physicians perceived POCUS to be very helpful in their clinical decision-making. These data support the need to advance POCUS education at the residency level throughout LMICs, which may be an ideal strategy to promote widespread utilization of POCUS in low-resource settings globally.
LncRNA MCM3AP-AS1 is downregulated in diabetic retinopathy and promotes cell apoptosis by regulating miR-211/SIRT1
Authors: Xia Zhaoxia , Yang Chaoying , Yang Xiaoxi , Wu Shuduan , Feng Zhizhen , Qu Lei , Chen Xianghua , Liu Linyu , Ma Yanling 
Published: May 2022

Abstract
Aim
This study aimed to investigate the role of lncRNA MCM3AP-AS1 in diabetic retinopathy (DR).

Methods
Plasma MCM3AP-AS1 levels in DR patients (n = 80), T2DM patients (n = 80), and Controls (n = 80) were measured by qPCR and compared using ANOVA (one-way) and Tukey test. The expressions of lncRNA MCM3AP-AS1 and miR-211 in Human retinal pigment epithelial cells (hRPE) line ARPE-19 were detected by RT-qPCR. Western blot and annexin V-FITC staining were performed to investigate the role of MCM3AP-AS1/SIRT1 in ARPE-19 cell proliferation and apoptosis in vitro.

Results
We observed that MCM3AP-AS1 was downregulated in DR patients 25 comparing to T2D patients without significantly complications. Bioinformatics analysis showed that MCM3AP-AS1 might bind miR-211. However, no significant correlation between these two factors was observed in DR patients. Consistently, overexpression of MCM3AP-AS1 and miR-211 failed to affect the expression of each other in hRPE. Interestingly, MCM3AP-AS1 overexpression upregulated SIRT1, a target of miR-211. Moreover, MCM3AP-AS1 was downregulated in DR patients compared to type 2 diabetic mellitus patients without significant complications. In RPEs, high glucose treatment downregulated MCM3AP-AS1. Cell apoptosis analysis showed that MCM3AP-AS1 and SIRT1 overexpression decreased the apoptotic rate of RPEs, and miR-211 overexpression reduced the effect of MCM3AP-AS1 and SIRT1 overexpression.

Conclusion
MCM3AP-AS1 is downregulated in DR and promotes cell apoptosis by regulating miR-211/SIRT1.