bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between tumor response to systemic therapy and patient-reported disease symptoms
Authors: Ito Yu , Awano Nobuyasu , Takahashi Naoko , Izumo Takehiro , Kunitoh Hideo 
Published: September 2022

Abstract

Background
The association of treatment-induced tumor shrinkage with symptom palliation for patients with lung cancer remains unknown. We investigated this correlation using the Edmonton Symptom Assessment System–Revised.

Methods
Using the in-hospital cancer registry, we identified patients receiving chemotherapy and/or immunotherapy for newly diagnosed advanced or metastatic lung cancer. Tumor response and post-treatment Edmonton Symptom Assessment System–Revised were obtained after 2–3 treatment cycles. Patients were divided into groups with or without &gt;30% unidirectional tumor shrinkage (objective response [OR] or non-OR [N-OR] groups, respectively). They were further classified as good-objective response (&gt;50% unidirectional tumor shrinkage), moderate-objective response (30–50% shrinkage), progressive disease (&gt;20% tumor growth or new lesion) or stable disease (SD; N-objective response and non-progressive disease). The primary endpoint was change in the total Edmonton Symptom Assessment System–Revised score from baseline. The Mann–Whitney U test was used for analysis.

Results
In total, 113 patients were enrolled. The total Edmonton Symptom Assessment System–Revised score was significantly more improved in the OR group versus the N-OR group (median: 5 vs. 2, respectively; P = 0.013). This association was more prominent in patients with small-cell lung cancer and large-cell neuroendocrine tumor than those with other histology. Sensitivity analyses showed that the total Edmonton Symptom Assessment System–Revised score was more improved in the OR group versus the SD group (median: 5 vs. 3, respectively; P = 0.029) and in the ‘good-OR’ group versus the ‘moderate-OR and SD’ group (median: 7.5 vs. 2, respectively; P = 0.003), suggesting that greater tumor shrinkage led to more symptom amelioration.

Conclusions
Tumor shrinkage was associated with Edmonton Symptom Assessment System–Revised score improvement in patients with lung cancer receiving systemic therapy.
Chronological Changes in the Expression Pattern of Hippocampal Prion Proteins During Disease Progression in Sporadic Creutzfeldt-Jakob Disease MM1 Subtype
Authors: Yagita Kaoru , Noguchi Hideko , Koyama Sachiko , Hamasaki Hideomi , Komori Takashi , Aishima Shinichi , Kosaka Takayuki , Ueda Mitsuharu , Komohara Yoshihiro , Watanabe Akihiro , Sasagasako Naokazu , Ninomiya Toshiharu , Oda Yoshinao , Honda Hiroyuki 
Published: September 2022

Abstract
The differential effects of sporadic Creutzfeldt-Jakob disease (sCJD) on the hippocampus and other neocortical areas are poorly understood. We aimed to reveal the histological patterns of cellular prion protein (PrPC) and abnormal prion protein (PrPSc) in hippocampi of sCJD patients and normal controls (NCs). Our study examined 18 postmortem sCJD patients (MM1, 14 cases; MM1 + 2c, 3 cases; MM1 + 2t, 1 case) and 12 NCs. Immunohistochemistry was conducted using 4 primary antibodies, of which 3 targeted the N-terminus of the prion protein (PrP), and 1 (EP1802Y) targeted the C-terminal domain. PrPC expression was abundant in the hippocampus of NCs, and the distribution of PrPC at CA3/4 was reminiscent of synaptic complexes. In sCJD cases with a disease history of &lt;2 years, antibodies against the N-terminus could not detect synapse-like PrP expression at CA4; however, EP1802Y could characterize the synapse-like expression. PrPSc accumulation and spongiform changes became evident after 2 years of illness, when PrPSc deposits were more noticeably detected by N-terminal-specific antibodies. Our findings highlighted the chronology of histopathological alterations in the CA4 region in sCJD patients.
Local voluntary reports: the implementation of sustainable development goals in northern and southern cities
Authors: Ruiz-Campillo Xira , Rosas Nieva Samanta 
Published: September 2022

Abstract
Given the increasing relevance of cities in the global agenda, we examine the voluntary local reports from six northern and southern cities around the world to understand their approach to the 2030 Agenda for sustainable development. We examine not only the framework but also the content of the reports to identify the differences in reporting on sustainable development goals (SDGs) and the factors that may explain why these cities have voluntarily submitted their reports. The research has revealed a wide diversity in the structure and content of the voluntary local reports, demonstrating that there was little to no institutional framework used to submit and compile the reports. Although the reports of northern cities tend to align with previous strategies for the SDGs, the implementation of the 2030 Agenda in southern cities has had a more significant impact on the adoption of monitoring and evaluation mechanisms at the local level. We have found that both international bodies and national policies have an influence on the development of sustainable practices at the local level. Our analysis also indicates that all cities have some sort of international exposure either through their participation in transnational municipal networks or through their collaboration with international organizations, especially in southern cities, which can explain why these cities (and not others) are more active in the adoption of SDGs at the local level and in the submission of voluntary reports.
A Synagogue Center Grows in Tel Aviv: On Glocalization, Consumerism and Religion
Authors: Libel-Hass Einat , Ferziger Adam S 
Published: September 2022

Abstract
Alongside the ongoing dominance of Orthodox Judaism in Israel, novel liberal religious frameworks have emerged that seek to address the needs of various constituencies through innovative approaches to synagogue life. One of the most active and successful is the Reform “Beit-Daniel” in Tel- Aviv, Israel's urban epicenter. In this article, the institutional structure that emerged in Beit-Daniel is compared with another neighboring synagogue, the Conservative/Masorti, Tiferet Shalom. Both adapted two well-established elements of the American Jewish experience—the synagogue-center and the “Rabbi as CEO”—and applied them to the Tel-Aviv environment. Yet Beit-Daniel has been more impactful. We examine its unique formula in the context of broader trends relating to religion in Israeli society since the late twentieth century, along with the particular features of Tel Aviv's urban environment. Tel-Aviv is a predominantly secular milieu with a strong consumerist orientation. By homing in on these features, Beit-Daniel's leadership facilitated a novel Israeli framework that operates as a religious, educational, and cultural service provider for a heterogeneous spectrum of target populations, a “community of communities” grounded on its diverse network. The data provided in this article was collected during four years of ethnographic fieldwork at Tel-Aviv's liberal congregations.
A 3′ UTR-derived small RNA connecting nitrogen and carbon metabolism in enteric bacteria
Authors: Walling Lauren R , Kouse Andrew B , Shabalina Svetlana A , Zhang Hongen , Storz Gisela 
Published: September 2022

Abstract
Increasing numbers of small, regulatory RNAs (sRNAs) corresponding to 3′ untranslated regions (UTR) are being discovered in bacteria. One such sRNA, denoted GlnZ, corresponds to the 3′ UTR of the Escherichia coli glnA mRNA encoding glutamine synthetase. Several forms of GlnZ, processed from the glnA mRNA, are detected in cells growing with limiting ammonium. GlnZ levels are regulated transcriptionally by the NtrC transcription factor and post-transcriptionally by RNase III. Consistent with the expression, E. coli cells lacking glnZ show delayed outgrowth from nitrogen starvation compared to wild type cells. Transcriptome-wide RNA–RNA interactome datasets indicated that GlnZ binds to multiple target RNAs. Immunoblots and assays of fusions confirmed GlnZ-mediated repression of glnP and sucA, encoding proteins that contribute to glutamine transport and the citric acid cycle, respectively. Although the overall sequences of GlnZ from E. coli K-12, Enterohemorrhagic E. coli and Salmonella enterica have significant differences due to various sequence insertions, all forms of the sRNA were able to regulate the two targets characterized. Together our data show that GlnZ impacts growth of E. coli under low nitrogen conditions by modulating genes that affect carbon and nitrogen flux.
RMDisease V2.0: an updated database of genetic variants that affect RNA modifications with disease and trait implication
Authors: Song Bowen , Wang Xuan , Liang Zhanmin , Ma Jiongming , Huang Daiyun , Wang Yue , de Magalhães João Pedro , Rigden Daniel J , Meng Jia , Liu Gang , Chen Kunqi , Wei Zhen 
Published: September 2022

Abstract
Recent advances in epitranscriptomics have unveiled functional associations between RNA modifications (RMs) and multiple human diseases, but distinguishing the functional or disease-related single nucleotide variants (SNVs) from the majority of ‘silent’ variants remains a major challenge. We previously developed the RMDisease database for unveiling the association between genetic variants and RMs concerning human disease pathogenesis. In this work, we present RMDisease v2.0, an updated database with expanded coverage. Using deep learning models and from 873 819 experimentally validated RM sites, we identified a total of 1 366 252 RM-associated variants that may affect (add or remove an RM site) 16 different types of RNA modifications (m6A, m5C, m1A, m5U, Ψ, m6Am, m7G, A-to-I, ac4C, Am, Cm, Um, Gm, hm5C, D and f5C) in 20 organisms (human, mouse, rat, zebrafish, maize, fruit fly, yeast, fission yeast, Arabidopsis, rice, chicken, goat, sheep, pig, cow, rhesus monkey, tomato, chimpanzee, green monkey and SARS-CoV-2). Among them, 14 749 disease- and 2441 trait-associated genetic variants may function via the perturbation of epitranscriptomic markers. RMDisease v2.0 should serve as a useful resource for studying the genetic drivers of phenotypes that lie within the epitranscriptome layer circuitry, and is freely accessible at: www.rnamd.org/rmdisease2.
KS01.4.A Transcriptional evolution of glioblastoma point towards changes in bulk composition, mesenchymal sub-type as end-state, and a prognostic association with increased extracellular matrix gene expression
Authors: Hoogstrate Y , Draaisma K , van Hijfte L , Ghisai S A , de Heer I , de Heer I , van den Bent M J , Robe P A , French P J 
Published: September 2022

Abstract

Background
Glioblastoma is the most prevalent and severe type of malignant brain tumor in adults. Although the genetic make-up initiating glioblastoma is increasingly better understood, a better understanding in the mechanisms that drive its evolution, heterogeneity and therapy resistance may reveal new directions for therapy development. To get better insights into glioblastoma evolution, we analyzed and deconvoluted transcriptomes of primary and recurrent glioblastoma resections.

Material and Methods
Matching primary and secondary resections from n=185 uniformly treated glioblastoma patients were collected as part of EORTC Study 1542 and RNA-sequenced. Data was extended with pairs from n=51 patients from the GLASS study. The datasets were subjected to differential and deconvolution analysis using in-house algorithms.

Results
When projecting the tumor samples into a reduced Glioblastoma Intrinsic Transcriptional Subtype space, visualization of transitions indicated that the CL subtype switches most often. As we found no more transitions from MES to other subtypes than to be expected by chance, we concluded that MES is an end-state. On average, tumor purity percentages decreased from ~67% to ~50%, mostly due to an increase in macrophages/microglia. Differential expression analysis was performed with correction for the fraction of non-malignant cells. While expression of glioblastoma associated oncogenes did not change significantly over time, marker genes for macrophages/microglia, neurons and oligodendrocytes were up-regulated whereas endothelial cell markers were down-regulated. A cluster of ~30 extracellular matrix associated (ECM) genes increased significantly over time. Single cell RNA-seq, IF-staining and RNA-ish indicated the signature is most strongly expressed near intra-tumoral vessels. Since endothelial marker genes were down-regulated over time, this suggests a form of prognostic vessel progression with a representative transcriptome signature.

Conclusion
Using a large cohort and validation set of uniformly treated patients, we demonstrate how the glioblastoma transcriptome changes over time with in particular changes the composition of the tumor and its environment. The tumor purity decrease over times suggests a more invasive phenotype or recruitment of non-malignant cells or a combination of both. A post-progression increase in expression of ECM-associated genes expressed near blood vessels was associated with poor survival. Concluding, while no consistent path for transcriptional evolution of tumor cells was observed other than transitions to the MES subtype, glioblastoma becomes more aggressive in time by (re-)organizing its environment.
KS02.6.A Application of positive psychology intervention in continuous quality improvement of nursing care for patients with intracranial tumor
Authors: Bai L , Zhou Z 
Published: September 2022

Abstract

Background
To investigate the effect of positive psychology intervention on continuous quality improvement of nursing care for patients with intracranial tumor

Material and Methods
A total of 124 patients with intracranial tumor who received surgical treatment in our hospital from October 2020 to December 2021 were selected by convenience sampling method, and the patients were randomly divided into control group and intervention group.Patients in the control group received continuous quality improvement of postoperative routine care, and patients in the intervention group received positive psychological intervention based on postoperative routine care. Postoperative treatment compliance, subjective well-being and daily living ability were compared between the two groups

Results
The excellent compliance rate of intervention group was 95.15%, significantly higher than that of control group 83.87% (P &lt; 0.05). The subjective well-being in intervention group (56.73±3.42) was significantly higher than that in control group (53.81±2.76) (P &lt; 0.05). There was no significant difference in daily living ability score between 2 groups before intervention (P &gt; 0.05). After intervention, the daily living ability score of the intervention group was (57.49±9.16), which was significantly higher than that of the control group and before intervention (P &lt; 0.05).

Conclusion
Positive psychology intervention for patients with intracranial tumor can effectively improve the quality of nursing, increase the patient's treatment compliance, and improve the subjective well-being of patients and the ability of daily life is of great significance.
KS02.7.A Impact of FET PET on multidisciplinary neurooncological tumor board decisions in patients with brain tumors
Authors: Ceccon G S , Werner J , Ruge M I , Goldbrunner R , Celik E , Baues C , Deckert M , Brunn A , Büttner R , Golla H , Nogova L , Schlamann M , Kabbasch C , Rueß D , Hampl J , Wollring M , Bauer E K , Tscherpel C , Fink G R , Langen K , Galldiks N 
Published: September 2022

Abstract

Background
Following neurooncological treatment of brain tumors, neurooncologists are often confronted with equivocal MRI findings (e.g., treatment-related changes such as pseudoprogression, non-measurable contrast-enhancing lesions, T2/FLAIR signal alterations, pseudoresponse). Especially in Europe, amino-acid PET is increasingly integrated into multidisciplinary neurooncological tumor boards (MNTB) to overcome these diagnostic uncertainties. We evaluated the correctness of MNTB decisions, in which amino acid PET findings were taken into account.

Material and Methods
In a single-university center study, we retrospectively evaluated 182 MNTB decisions of 154 patients with histomolecularly defined WHO grade 3 or 4 gliomas (n=123), including glioblastoma (n=80), anaplastic glioma (n=42), and gliosarcoma (n=1), or brain metastases (n=31) secondary to lung cancer, melanoma, breast cancer, or colorectal cancer presenting equivocal MRI findings following anticancer treatment. All patients underwent O-(2-[18F]-fluoroethyl)-L-tyrosine (FET) PET imaging as an adjunct for decision-making. Additionally, the patients’ clinical status, pretreatment, and conventional MRI findings were considered for decision-making. The presence of neoplastic tissue was considered if the mean FET uptake as assessed by tumor-to-brain ratios was &gt; 2.0. MNTB decisions were validated using the neuropathological result in 42% (n=77) or clinicoradiologically in 58% (n=105). The diagnostic performance of MTNB decisions was evaluated using 2x2 contingency tables.

Results
The validation of all 182 MNTB recommendations, which integrated FET PET in the decision-making process, were correct in 95% (sensitivity, 97%; specificity, 75%; positive predictive value, 96%). Due to tumor progression, MNTB recommendations prompted a treatment change in 88% (n=160 of 182 decisions). When FET PET findings suggested progressive disease (n=157), MNTB decisions were correct in 96% (positive predictive value, 97%). In 22 MNTB decisions with the recommendation to continue the current treatment regimen, 82% were correctly identified as treatment-related changes.

Conclusion
FET PET seems to have a significant impact on MNTB decisions. A prospective evaluation of MNTB decisions with and without the integration of FET PET is warranted to define the added value of FET PET.
KS05.5.A Alterations in white matter fiber density associated with structural MRI and metabolic PET lesions following multimodal therapy in glioma patients
Authors: Friedrich M , Farrher E , Caspers S , Lohmann P , Stoffels G , Filss C , Weiss Lucas C , Ruge M I , Langen K J , Shah N J , Fink G R , Galldiks N , Kocher M 
Published: September 2022

Abstract

Background
In glioma patients, multimodal therapy and recurrent tumor result in local brain tissue changes, characterized by pathologic findings in structural MRI and metabolic PET images. Little is known about these different lesion types’ impact on the local white matter fiber architecture and clinical outcome.

Patients and Methods
This study included data from 121 pretreated patients (median age, 52 years; ECOG, 01) with histomolecularly characterized glioma (WHO grade IV glioblastoma, n=81; WHO grade III anaplastic astrocytoma, n=28; WHO grade III anaplastic oligodendroglioma, n=12), who had a resection, radiotherapy, alkylating chemotherapy, or combinations thereof. After a median time of 14 months (range, 1-214 months), post-therapeutic structural and metabolic findings were evaluated using anatomical MRI and O-(2-[18F]fluoroethyl)-L-tyrosine (FET) PET acquired on a 3T hybrid PET/MR scanner. Local fiber density was estimated from tractography based on highangular resolution diffusion-weighted imaging. A cohort of 121 healthy subjects selected from the 1000BRAINS study and matched for age, gender and education served as a control group.

Results
The median volume of resection cavities, contrast-enhancing regions, regions with pathologically increased FET uptake, and T2/FLAIR hyperintense regions amounted to 20.9, 7.9, 30.3, and 53.4 mL, respectively. Compared to the control group, the average local fiber density in these regions was significantly reduced (p&lt;0.001). Resection cavities showed the highest reduction, followed by contrast-enhancing lesions and metabolically active tumors on FET PET (relative fiber density reduction, -87%, -65%, -55%, respectively). The local fiber density was inversely related (p=0.005) to the FET uptake in recurrent tumors. T2/FLAIR hyperintense lesions, either assigned to peritumoral edema in recurrent glioma or radiation-induced gliosis, had a comparable impact on reducing fiber density (48% and 41%, respectively). The total fiber loss (average fiber loss multiplied by lesion volume) associated with contrast-enhancing lesions (p=0.006) and T2/FLAIR hyperintense lesions (p=0.013) had a significant impact on the general performance status of the patients (ECOG score).

Conclusions
Our results suggest that apart from resection cavities, reduction in local fiber density is greatest in contrast-enhancing recurrent tumors, but total fiber loss induced by edema or gliosis has an equal detrimental effect on the patients’ performance due to the larger volume affected.

Funding
Funded by the 1000BRAINS study (INM, Research Centre Juelich, Germany), Horizon 2020 (Grant No. 945539 (HBP SGA3; SC)), and Heinz Nixdorf Foundation.
JS03.5.A Pediatric Neurofibromatosis type 1- associated Malignant Peripheral Nerve Sheath Tumors: a national experience
Authors: Silva E M , Nunes S , Luz I , Mohanlal U , Passos J 
Published: September 2022

Abstract

Background
Malignant peripheral nerve sheath tumors (MPNST) are rare soft tissue sarcomas and although less that 10% occur in the pediatric age, they are the most feared complication in the follow-up of Neurofibromatosis Type 1 (NF1) patients. NF1-children tend to have larger tumors and worse prognosis than non-NF1 patients. There is a lack of data regarding MPNST in pediatric populations with NF1, and the present work aims to characterize a Portuguese population of pediatric NF1 patients who developed a MPNST.

Material and Methods
Retrospective analysis of all NF1-pediatric patients diagnosed with MPNST between 2000 to 2021, from three centers in Portugal. Patient characteristics, treatment modalities and clinical outcomes were reviewed.

Results
12 patients (6 males and 6 females) met the inclusion criteria. 7 had no family history of NF1. Median age at diagnosis was 14.1 years (range 10-18). 5 had been diagnosed previously with Plexiform Neurofibroma and were treated with selumetinib (2) and partial surgery (2). MPNSTs were mostly located in the retroperitoneum (7) and in the proximal lower limbs (2). Only 1 patient had a resectable tumor at diagnosis and 3 patients had metastatic disease. In the FDG-PET scan of evaluated patients (7), the mean maximum standardized uptake value of the main lesion was 6 (range 3.6-9.7).Neoadjuvant ifosfamide plus doxorubicin chemotherapy was used in 4 patients. The median overall survival in this population was 10.3 months (95% CI 0.1-20.6) 2 patients remain alive.

Conclusion
The prognosis of MPNSTs in NF1 pediatric patients is very poor. In a subset of patients, MPNSTs develop from known Plexiform Neurofibromas. Current surveillance ant treatment protocols need to be improved.