bluuebunny/crossref_metadata_2025_split huggingface dataset:
LBODP077 The Therapeutic Potential Of Targeting Histone Deacetylase For Pituitary Neuroendocrine Tumors By High-throughout Drug Screening
Authors: Wu Zhe Bao , Cheng Yijun , Xie Jing , Xue Li , Dai Yuting 
Published: November 2022

Abstract

Background
A growing awareness regarding all molecular mechanisms involved in both tumor shrinkage and hormonal control might allow for medical treatment of pituitary neuroendocrine tumors (PitNETs), especially macroadenomas, rather than surgery. However, there are still no effectively therapeutic targets and corresponding drugs for all subtypes of PitNETs.

Methods
To identify druggable cell-intrinsic vulnerabilities and correspondingly promising therapeutic agents, we examined 3 PitNETs cell lines and 21 PitNETs patient-derived primary cell cultures in sequential quantitative high-throughput screens (HTS) of 2149 FDA-approved and bio-active targeted drugs. Furthermore, we verified the most effective drug which targeted the class of mechanistic vulnerabilities, histone deacetylase (HDAC) in vitro and in vivo PitNETs models. Further RNA sequencing was employed to reveal underlying molecular mechanisms following treatment with the most effective HDAC inhibitor, panobinostat.

Results
The HTS effort generated a total of 55296 single-agent dose responses which were enriched among multiple inhibitors for relevant PitNETs oncogenic targets, including HDAC, Akt/mTOR, proteasome, mitogenactivated protein kinase (MEK), and phosphoinositide-3-kinase (PI3K). Inhibitors from these mechanistic classes demonstrated a relatively wide potency-range, with HDAC inhibitors being, on average, the most potent drug class. Further in vitro and In vivo testing in PitNETs models validated HDACi, especially panobinostat as a promising therapeutic approach. Transcriptional surveys revealed substantial alterations to the oxidative redox mediated by the Akt/mTOR1/4EBP1/Nuclear factor-E2-related factor 2 (Nrf2) antioxidant signaling pathway following treatment with panobinostat.

Conclusion
The current study revealed a class of newfound anti-tumor drugs, HDACi based on the HTS technology, which may be potential drugs for the treatment of PitNETs.
Presentation: No date and time listed
Thyroglobulin Assay Interferences: Clinical Usefulness of Mass-Spectrometry Methods
Authors: Barbesino Giuseppe , Algeciras-Schimnich Alicia , Bornhorst Joshua 
Published: November 2022

AbstractContextThyroglobulin autoantibodies (TgAbs) affect thyroglobulin immunometric assays (TgIMAs), causing falsely low results. Conversely, heterophilic antibodies (HAs) may cause falsely elevated results. Thyroglobulin (Tg) measurements by mass spectrometry (MS) resist antibody interference. The most effective use of TgIMA/TgMS in the evaluation of Tg remains unclear.ObjectiveThe objective of this work was to study the usefulness of TgMS vs TgIMA in the presence of Tg measurement interference by HA and TgAb.MethodsIn 163 thyroid cancer patients, Tg was postoperatively measured by TgIMA and TgMS. When TgIMA was elevated and TgMS undetectable, HA was assessed by serial dilution and pretreatment with HA blocking reagent. TgIMA and TgMS were compared in TgAb-positive patients with well-characterized clinical status.Results6 out of 45 cases with TgIMA &gt;1 ng/mL had undetectable TgMS. HA interference was confirmed by serial dilution and HA blocking reagent addition. In TgAb-positive cases, TgIMA and TgMS were highly correlated (R2 = 0.86). In patients with structural disease and TgAb, TgIMA and TgMS were detectable in 6/19 patients, and 9/19 cases, respectively. The TgMS concentration range in the 3 discrepant cases ranged from 0.5 to 2.0 ng/mL. Hence, the presence of TgAb was associated with inappropriately reduced Tg concentrations with both TgIMA and TgMS.ConclusionHA cause falsely elevated TgIMA with undetectable TgMS with significant frequency. TgMS can be used to rule out HA interference. Albeit resistant to TgAb in vitro, TgMS detects little Tg in patients with TgAb and structural disease. Hence, TgAb may reduce Tg concentrations in vivo. The implication is that no assay design may be able to overcome this problem. TgMS may not detect structural disease in TgAb-positive patients.
Case Report: Caecal volvulus management from diagnosis to treatment in a young patient
Authors: Abbassi Imed , Triki Wissem , Trigui Racem , Ben Salah Ramy , Itaimi Ahmed , Ayed Karim , Sebri Hajer , Baraket Oussema , Bouchoucha Sami 
Published: November 2022

Caecal volvulus (CV) is a rare cause of intestinal obstruction, defined by an axial torsion of the caecum, ascending colon, and terminal ileum around the mesenteric vascular pedicles, leading to ischemia and bowel necrosis. A 20-year-old woman, with no significant medical history, was admitted for generalized abdominal pain evolving for three days, along with constipation and abdominal distension, but with no vomiting. Physical examination showed a generalized abdominal tenderness with no rigidity or rebound tenderness, associated with abdominal distension and tympanic upon percussion. Laboratory findings were within normal limits. An abdominal computed tomography scan revealed distension of a loop of the large bowel with its long axis extending from the right lower quadrant to the epigastrium or left upper quadrant. Colonic haustral pattern was absent. An abdominal computed tomography scan showed a rounded focal collection of air-distended bowel with haustral creases in the upper left quadrant. In addition, spiraled loops of the collapsed cecum (giving a whirl sign) were noted, along with low-attenuating fatty mesentery from the twisted bowel. The patient underwent an emergency laparotomy and caecectomy using GEA 80 charges. The patient had no complaints post-operation. CV is a rare cause of bowel obstruction, mainly caused by an exceedingly mobile caecum. Despite its rareness, CV represents the second most common cause of large bowel volvulus, behind sigmoid volvulus. For acute obstruction by CV, it is hard to differentiate it clinically from obstruction of the small bowel; therefore, radiological exams are needed. Surgery is the gold standard treatment for CV. We report a rare case of CV to highlight the rarity of this pathology, specify its diagnostic and therapeutic means, and its clinical and biological evolution.
Psychosocial- and disclosure-related challenges among HIV-serodiscordant couples: an interpretative phenomenological analysis study
Authors: Lelaka Constance Matshidiso , Tshivhase Livhuwani , Moyo Idah , Ndou Nthomeni Dorah , Mavhandu-Mudzusi Azwihangwizi Hellen 
Published: November 2022

Background: Serodiscordant refers to a couple where one has human immunodeficiency virus (HIV), and the other partner is HIV negative. HIV-serodiscordancy often results in diverse psychological and emotional challenges. Evidence demonstrates that the dynamics of living in an HIV-serodiscordant relationship are often stressful. This study explored the psychosocial- and disclosure-related challenges faced by couples in HIV-serodiscordant relationships in South Africa. Methods: An interpretative phenomenological analysis (IPA) design was followed. A total of 13 HIV-serodiscordant partners were purposively sampled. Data were collected through individual face-to-face interviews and analysed using the IPA framework. Results: The findings indicated that HIV-serodiscordant couples experienced diverse psychosocial challenges such as shock, sadness, hurt, denial and disbelief. Participants also experienced challenges such as selective disclosure and fear of further disclosure. Conclusions: The findings have implications for public health and are critical in programming and designing couple-based HIV care interventions. Couples in HIV-serodiscordant relationships would benefit more from differentiated, client-centred psychosocial support. To contribute to the HIV epidemic control and reduction of new HIV infections, specific interventions such as couples counselling, disclosure counselling, support groups, health education and safer conception risk reduction strategies need to be implemented.
Early detection of biliary atresia in primary health care: still a problem
Authors: Setyoboedi Bagus , Prihaningtyas Rendi Aji , Utomo Martono Tri , Arief Sjamsul 
Published: November 2022

Background: Biliary atresia is the leading cause of liver transplantation in children. Early detection of biliary atresia is crucial for diagnosis and disease progression. The purpose of this study was to analyze knowledge about biliary atresia and the effectiveness of health education in increasing the knowledge of primary health care providers. Methods: A quasi-experimental study with pretest and posttest designs was carried out in Sidoarjo, East Java using a self-administered questionnaire. The intervention using health education was delivered by pediatrician and consultant of pediatric gastro hepatology. There were 13 questions on the questionnaire, question numbers 1 to 6 were about normal and abnormal neonatal jaundice, question numbers 7 to 13 were about biliary atresia. Results: A total of 252 participants were involved, the mean age of the participants was 40.7 ± 9.4 years old. Most of the participants were midwives (61.9%) and 77.8% of participants have years of service in primary health care > 5 years. A total of 40.5% participants stated that newborns may have physiological jaundice, which was characterized by icteric sclera, pale stools, and dark urine. A total of 27,4% and 24,2% participants said that all jaundice in newborn will always improve on their own and newborn with prolonged jaundice does not need further examination, respectively. There was an increase in the median value in the pretest and posttest knowledge scores after interventional health education (p < 0,05). Conclusions: The primary health care provider understands about biliary atresia, however, the initial knowledge about early detection of biliary atresia is not evenly distributed in all primary health care providers. These findings suggest that improving knowledge to early detection of biliary atresia is needed. Health education can be used effectively in increasing knowledge about biliary atresia.
Intensive care nurses' knowledge of enteral nutrition at public hospitals in Sana'a, Yemen: a cross-sectional survey
Authors: Ali Hussein Al-Qalah Talal , Alrubaiee Gamil Ghaleb 
Published: November 2022

Background: Nurses have a pivotal role in initiating and managing enteral nutrition (EN) and monitoring any potential complications. Yet, it is unclear whether Yemeni nurses have adequate knowledge to deliver and manage enteral nutrition safely. Therefore, the aim of this study was to assess the level of ICU nurses’ knowledge regarding the care of EN. Methods: A descriptive cross-sectional study was conducted from February 2019 to March 2019. A probability sampling method was used to recruit 174 nurses from four public hospitals in the capital city of Yemen. A self-administered 17-item questionnaire related to ICU nurses' knowledge regarding EN intervention was used to collect the data. Results: Based on our findings, only 10.9% of the respondents had an adequate level of knowledge about EN care, while 43.1% of them had a moderate level of knowledge, and 46.0% of them had an inadequate level of knowledge. Only 16.1% of the participants were knowledgeable of EN pre-administration care, whereas 5.80% of them were knowledgeable of EN administration care, and 9.20% were knowledgeable of EN post-administration care. There were significant associations between ICU nurses’ level of knowledge of EN care and their level of education and knowledge sources. Conclusions: The significant gap in ICU nurses’ knowledge regarding EN care identified implies the need to upgrade and refresh of the ICU nurses' knowledge by implementing a regular training program concerning EN care.
Truthfulness of a Network Resource-Sharing Protocol
Authors: Cheng Yukun , Deng Xiaotie , Qi Qi , Yan Xiang 
Published: November 2022

We consider a sharing economy over a network in which each vertex agent allocates resources to its neighbors in response to their contributions. General equilibrium theory can be applied here to solve the problem of deciding how to fairly and efficiently allocate resources among agents as resource sharing over the network can be modeled as a pure exchange economy. It is known that proportional sharing dynamics converges to a market equilibrium solution. We are particularly interested in proportional sharing dynamics as a mechanism for network resource sharing. Our focus is on the key issue in internet market design: whether an agent may manipulate its report of its own private information to gain more resources under this mechanism. This work establishes the first mathematical proof that such a practical distributed network resource-sharing protocol is truthful against the manipulative strategies of feasible weight misreporting and edge deletion applied both individually and together. Funding: This work was supported by the National Nature Science Foundation of China [Grants 11871366, 62172012]; Beijing Outstanding Young Scientist Program [Grant BJJWZYJH012019100020098]; the Fundamental Research Funds for the Central Universities; the Research Funds of Renmin University of China [Grant 297522503709]; and the Intelligent Social Governance Platform, Major Innovation & Planning Interdisciplinary Platform for the “Double-First Class” Initiative, Renmin University of China.
Preliminary analysis of self-reported quality health indicators of patients on opioid agonist therapy at specialty and primary care clinics in Ukraine: A randomized control trial
Authors: Pashchenko Oleksandra , Bromberg Daniel J. , Dumchev Kostyantyn , LaMonaca Katherine , Pykalo Iryna , Filippovych Myroslava , Esserman Denise , Polonsky Maxim , Galvez de Leon Samy J. , Morozova Olga , Dvoriak Sergii , Altice Frederick L. 
Published: November 2022

International agencies recommend integrating addiction treatment into primary care for people who inject drugs (PWID) with opioid use disorder (OUD). Empirical data supporting integration that incorporates comprehensive health outcomes, however, are not known. For this randomized controlled trial in Ukraine, adult PWID with OUD were randomized to receive opioid agonist therapy (OAT) in specialty addiction treatment clinics (SATC) or to primary care clinics (PCCs). For those randomized to PCC, they were subsequently allocated to PCCs where clinicians received pay-for-performance (P4P) incentives (PCC with P4P) or not (PCC without P4P). Participating cities had one of each of the three intervention sites to control for geographic variation. Ongoing tele-education specialty training (OAT, HIV, tuberculosis) was provided to all PCCs. While the primary outcome for the parent trial focuses on patient medical record data, this preliminary analysis focuses on assessment of self-reported achievement of nationally recommended quality health indicators (QHIs) which is summed as a composite QHI score. Secondary outcomes included specialty and primary care QHI subscores. This study occurred from 01/20/2018-11/1/2020 with 818 of 990 randomized participants having complete self-reported data for analysis. Relative to SATC (treatment as usual), the mean composite QHI score was 12.7 (95% CI: 10.1–15.3; p<0.001) percentage points higher at PCCs; similar and significantly higher scores were observed in PCCs compared to SATCs for both primary care (PCC vs SATC: 18.4 [95% CI: 14.8–22.0; p<0.001] and specialty (PCC vs SATC: 5.9 [95% CI: 2.6–9.2; p<0.001] QHI scores. Additionally, the mean composite QHI score was 4.6 (95% CI: 2.0–7.2; p<0.001) points higher in participants with long term (>3 months) experience with OAT compared to participants newly initiating OAT. In summary, PWID with OUD receive greater primary care and specialty healthcare services when receiving OAT at PCCs supported by tele-education relative to treatment as usual provided in SATCs.
Clinical trial registration: This trial was registered at clinicaltrials.gov and can be found using the following registration number: NCT04927091.
Prevalence and associated factors of COVID-19 among Moroccan physicians: A cross-sectional study
Authors: Baldi Marwa El , Laghrissi Amina , Marso Zakia , Chellat Fatima Zahra , Berraho Mohamed , Tachfouti Nabil , Fakir Samira El , Mellas Soufiane , Fahd Amar Mohamed , kohen Jamal , Boulaguige Fouad , Naamane Jamal , Lahmadi Khalid , Rhazi Karima El 
Published: November 2022

BackgroundCoronavirus disease (COVID-19) has emerged and spread rapidly worldwide and established a global public health crisis in early 2020. The first Moroccan case was reported on March 2, 2020. Since then, healthcare workers (HCWs) played a major role in saving human lives threatened by COVID-19. This study aimed to assess the prevalence of COVID-19 infection among Moroccan physicians and to report associated risk factors prior vaccination campaign.MethodsA cross-sectional study was carried out in the Fez-Meknes region of Morocco, 545 physicians’ data was collected using a self-reported online questionnaire. The data collection was done between December 1, 2020, and February 1, 2021.ResultsThe prevalence of COVID-19 among physicians was 27.3%. The mean age of the confirmed COVID-19 group was 38.4±12.9 years old. There was no association between COVID-19 infection and preventive measures compliance by physicians and healthcare authorities in the workplace. However, multivariate analysis strengthened the following factors such as increased risk of COVID-19 infection within men ☯aOR:1.896; 95% IC 1.272–2.828; p = 0.002]; the presence of at least one comorbidity ☯aOR:2.268; 95%IC 1.414–3.637; p = 0.001]; and working at a university or military hospitals ☯aOR:2.578; 95%IC 1.667–3.989; p = 0.001].ConclusionThis study allows comparing COVID-19 prevalence among healthcare workers before and after vaccination programs. This should support better preparation strategy for any future pandemics with appropriate and increased awareness for men, carrying comorbidity, and working environment with high COVID-19 disease management.
The Problems of Reading Arabic Text in Terms of Phonological Aspects (Case Study in Fifth Grade Students of SD Qurrota A’yun Babadan Bantul DIY Academic Year 2020/2021)
Authors: Arina Nur Sofiana  , Nur Hapsari Paramitha  , Nurul Huda  
Published: November 2022

This study aims to determine the problems of fifth-grade students of SD Qurrota A'yun in reading Arabic texts in terms of phonological aspects, to find out the efforts made by Arabic teachers in overcoming these problems, and to find out the ideal concept of learning Arabic phonology at the elementary school level. This research is a case study and field research conducted at SD Qurrota A'yun, Babadan, Bantul, DIY. The data collection used is the acquisition of qualitative data. The research subjects were the principal, Arabic teacher, and fifth-grade students of SD Quurota A'yun. Sampling was carried out using non-probability sampling in the form of purposive samples (purposed samples). They are collecting data using the method of observation, interviews, and documentation. The results of the study indicate that there are problems with reading Arabic text in students' Arabic learning in terms of phonology, are consists of phonetic aspects (in the form of vocal and consonant problems) and phonemic aspects (in the form of incorrect phonological pronunciation so that it can change the meaning of Arabic vocabulary). For this reason, teachers try to overcome this by collaborating Arabic lessons with Al-Qur'an lessons. The ideal concept of learning Arabic phonology at the elementary school level is inseparable from learning the Qur'anic tahsin.
Keywords: Reading Problems, Arabic Language, Arabic Text, Phonology.
Abstrak
Penelitian ini bertujuan untuk mengetahui problematika siswa kelas V SD Qurrota A’yun dalam membaca teks Arab ditinjau dari aspek fonologi, untuk mengetahui usaha yang dilakukan guru bahasa Arab dalam mengatasi problematika tersebut, dan untuk mengetahui konsep ideal pembelajaran fonologi bahasa Arab pada tingkat sekolah dasar. Penelitian ini merupakan penelitian studi kasus dan lapangan yang dilakukan di SD Qurrota A’yun, Babadan, Bantul, DIY. Pemerolehan data yang digunakan yaitu dengan pemerolehan data kualitatif. Subjek penelitian yaitu kepala sekolah, guru bahasa Arab, dan peserta didik kelas V SD Quurota A’yun. Pengambilan sampel dilakukan dengan menggunakan non probability sampling berup purposive sample (sampel bertujuan). Pengumpulan data menggunakan metode observasi, wawancara, dan dokumentasi. Hasil penelitian menunjukkan bahwa terdapat problematika membaca teks Arab dalam pembelajaran bahasa Arab siswa ditinjau dari segi fonologi yaitu terdiri dari aspek fonetik (berupa problematika vokal dan konsonan) dan aspek fonemik (berupa pengucapan fonologi yang kurang tepat sehingga bisa merubah makna dari kosakata bahasa Arab). Untuk itu, guru berupaya mengatasi hal tersebut dengan mengkolaborasikan pelajaran bahasa Arab dengan pelajaran Al-Qur’an. Konsep ideal pembelajaran fonologi bahasa Arab pada tingkat sekolah dasar yaitu tidak lepas dari pembelajaran tahsin Al-Qur’an.
Keywords: Problematika Membaca, Bahasa Arab, Teks Arab, Fonologi.