bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microglia senescence is related to neuropathic pain–associated comorbidities in the spared nerve injury model
Authors: Borgonetti Vittoria , Galeotti Nicoletta 
Published: November 2022

Abstract
The increased presence of senescent cells in different neurological diseases suggests the contribution of senescence in the pathophysiology of neurodegenerative disorders. Microglia can adapt to any type of disturbance of the homeostasis of the central nervous system, and its altered activity can lead to permanent and unresolvable damage. The aim of this work was to characterize the behavioural phenotype of spared nerve injury mice and then associate it with senescence-related mechanisms. In this work, we investigated the timing of the onset of anxiety, depression, or memory decline associated with peripheral neuropathic pain and their correlation with the presence of microglial cellular senescence. Spared nerve injury mice showed a persistent pain hypersensitivity from 3 days after surgery. Twenty-eight days after nerve injury, they also developed anxiety, depression, and cognitive impairment. The appearance of these symptoms was coincident to a significant increase of senescence markers, such as β-galactosidase and senescent-associated secretory phenotype, at the microglial level in the spinal cord and hippocampus of spared nerve injury animals. These markers were unaltered at previous time points. In murine immortalized microglial cells (BV2) stimulated with LPS 500 ng/mL for 10 days (4 hours/day) every other day, we observed an increase of β-galactosidase and senescent-associated secretory phenotype appearance, a reduction of cell viability, and an increase of senescence-associated heterochromatin foci. Therefore, present findings could represent an important step to a better understanding of the pathophysiological cellular mechanisms in comorbidities related to neuropathic pain states.
Association of systemic inflammatory and autoimmune manifestations with myelodysplastic syndromes: A systematic review and meta-analysis
Authors: Liang Qian , Zhao Jingyu , Zhang Lele , Gao Zhen , Pan Hong , Fang Liwei , Shi Jun 
Published: November 2022

Background:
Systemic inflammatory and autoimmune manifestations (SIAMs) are frequently reported in Myelodysplastic syndromes (MDS). Studies focused on the impact of SIMAs on survival outcomes of MDS remains controversial. We performed this systematic review and meta-analysis to determine the association of SIAMs with overall survival, median survival, rate of acute myeloid leukemia transformation and mortality of MDS.

Materials and methods:
An electronic search was conducted in 4 databases without any language restrictions, including PubMed, EMBASE, Medicine and Cochrane library up to April 30, 2021.

Results:
The 18 studies included a total of 4603 MDS patients, of which 1175 (25.5%) patients had SIAMs. MDS patients with SIAMs had a statistically shorter overall survival compared with patient without SIAMs (Hazard ratio, 2.43; 95% confidence interval [CI], 1.34–4.41; P < .01). Our results were most compatible with no effect of SIAMs on median survival, rate of acute myeloid leukemia transformation and mortality (Median survival ratio, 1.16; 95% CI, 0.91–1.47; Odds ratio, 0.96; 95% CI, 0.63–1.45 and 1.2; 95% CI, 0.84–1.7, respectively).

Conclusion:
In this systematic review and meta-analysis, SIAMs appeared to have an adverse effect on overall survival of MDS patients. This finding suggested that SIAMs may be a potential independent prognostic factor for MDS.
A randomized, double-blind, placebo-controlled multicenter clinical trial of Xiehuang Jiejing granule in the treatment of cough variant asthma in children
Authors: Qiao Yi-Na , Lin Shuang-Zhu , Duan Xiao-Zheng , Yang Ming-Hang , Zhang Xiao-Fang , Li Jing-Jing , Kang Sai-Nan , Wang Yu-Ting , Zhang Ying , Feng Xiao-Chun 
Published: November 2022

Background:
Cough variant asthma (CVA), also called concealed asthma or allergic asthma, is the most common cause of chronic cough in children. The disorder is mainly characterized by a nonproductive dry cough associated with a high recurrence rate that is conventionally treated with antibiotics, anti-inflammatory medications, cough suppressants, or expectorants. For millennia, Chinese herbal medicine (CHM) has been used widely in China to treat pediatric CVA cases, although high-quality evidence of CHM efficacy is lacking. In this study, the effectiveness and safety of Xiehuangjiejing (XHJJ) granule will be evaluated when used alone to treat children with CVA.

Methods and analysis:
A randomized, double-blind, parallel, placebo-controlled multicenter trial will be conducted over the course of 2 weeks. A total of 180 CVA patients of ages between 4 and 7 years old will be randomly assigned to the experimental group (XHJJ granules, 4.5 g administered 3 times daily) or control group (matched placebo, 4.5 g administered 3 times daily) in a 2:1 ratio based on subject number per group, respectively. The trial will consist of a 7-day medical interventional stage and a 7-day follow-up stage. On day 7 of the follow-up stage, an evaluation of all subjects will be carried out to assess cough symptom score as the primary outcome and several secondary outcomes, including TCM (traditional Chinese medicine) syndrome score, lung function, and dosage of salbutamol aerosol inhaler therapy. Safety assessments will also be evaluated during the trial.

Discussion:
The aim of this study was to examine the effectiveness and safety of Xiehuangjiejing (XHJJ) granule using a trial protocol designed to yield high-quality, statistically robust results for use in evaluating CHM as a treatment for CVA in children.
Prediction of network pharmacology and molecular docking-based strategy to determine potential pharmacological mechanism of Liuwei Dihuang pill against tinnitus
Authors: Wu Zhongbiao , Zhu Zhongyan , Cao Jian , Wu Weikun , Hu Shiping , Deng Chengcheng , Xie Qiang , Huang Xinmei , You Chengkun 
Published: November 2022

Background:
Liuwei Dihuang Pill is widely used to treat tinnitus in China. However, the underlying mechanism of Liuwei Dihuang Pill in treating tinnitus still remains unclear.

Objective:
To explore the potential pharmacological mechanism of Liuwei Dihuang Pill in the treatment of tinnitus based on network pharmacology and molecular docking.

Methods:
The active components of the Liuwei Dihuang Pill were obtained from the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) database. Cytoscape software was used to draw the active component-target network diagram of Liuwei Dihuang Pill, and obtain the core components. Then the corresponding targets were also obtained from the TCMSP database. Targets related to tinnitus were obtained from the GeneCards, DisGeNET, TTD and DrugBank databases. The String database was used to construct protein–protein interaction (PPI) network of common targets of drugs and diseases, then the core targets were screened out. The Annotation, Visualization and Integrated Discovery (DAVID) database was used for gene ontology (GO) enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis of common targets. Finally, the molecular docking between the core component and the core target was carried out by AutoDock.

Results:
The core components of Liuwei Dihuang Pill in the treatment of tinnitus including quercetin, stigmasterol, kaempferol, β-sitosterol, tetrahydroalstonine, which may act on core targets such as STAT3, transcription factor AP-1 (JUN), tumor necrosis factor (TNF), interleukin-6 and MAPK3. HIF-1 signaling pathway, Influenza A, P53 signaling pathway, and Toll-like receptor signaling pathway play a role in anti-inflammatory, improving microcirculation in the blood-labyrinth barrier, increasing cochlear blood flow, and preventing hair cell damage. The molecular docking results showed that the affinity between core components and core targets was good.

Conclusion:
The potential mechanism of Liuwei Dihuang Pill in the treatment of tinnitus was preliminarily discussed in this study, which may provide a theoretical basis and evidence for further experimental research.
Prognostic value of tumor size in thymic epithelial tumors: A systematic review and meta-analysis
Authors: Shao Yifeng , Tang Mingbo , Fang Linan , Wei Shixiong , Gao Xinliang , Liu Wei 
Published: November 2022

Background:
Whether the size of thymic epithelial tumors (TETs) has an impact on prognosis has long been a controversial issue. Our study was designed to investigate the value of tumor size in the prognosis (overall survival (OS) and relapse-free survival) of patients with TETs.

Methods:
We searched the databases such as PubMed, EMBASE, Web of Science, and clinical trials registration system for articles illustrating the impact of tumor size on survival data in TETs patients. We did a meta-analysis for OS and relapse-free survival.

Results:
We recruited 9 studies in our meta-analysis. Our study illustrates that TETs patients with small tumor size had better relapse-free survival (hazard ratio = 1.66, 95% confidence interval 1.18–2.35, P = .004) and OS (hazard ratio = 1.93, 95% confidence interval 1.30–2.80, P = .001) in comparison to patients with large tumor size.

Conclusions:
In conclusion, the results of our meta-analysis showed that TET size was significantly associated with overall and relapse-free survival of patients, with relatively small tumors tending to have a better prognosis.
A review and meta-analysis of the survival rate of adult with osteonecrosis of the femoral head treated with transtrochanteric rotational osteotomy
Authors: Xu Yong , Zeng Ping 
Published: November 2022

Background:
Osteonecrosis of the femoral head (ONFH) is a progressive disease, and transtrochanteric rotational osteotomy (TRO) is a promising option to treat hip preservation. Compared with outcomes of non-Asian patients, those of Asian patients are different. Once ONFH has progressed to the stage of collapse, total hip arthroplasty (THA) is an unavoidable surgical procedure. However, patients treated with TRO still have a certain rate of femoral head collapse. A meta-analysis was done to comprehensively understand the outcome of TRO surgery for ONFH, in which conversion to THA was used as an endpoint event or the survival rate of patients with ONFH after TRO surgery as an indicator.

Methods:
We retrieved electronic databases from the inception of the study until June 2022, using the survival rate after TRO surgery or that after conversion to the endpoint event of THA as the effect indicator. The Risk Difference Independent unmatched samples of counting information with 95% confidence intervals (CI) were used to calculate the outcome. Also, subgroup analysis was carried out for Asian and non-Asian patients. Heterogeneity and publication bias analyses were also done for the included studies

Results:
We pooled 19 studies, all of which were about applying TRO procedure for ONFH. There were 15 cohort studies, 4 case-control studies, and no randomized controlled studies. Based on the information extracted from the reported above (we extracted the relevant independent effect values separately for the case-control studies), this meta-analysis was performed based on a fixed-effect model, and META analysis was performed for an independent unpaired group of the samples. The total hip survival rate after TRO in ONFH was 0.58(95% CI = 0.45–0.72), The survival rate for Asians was: 0.68 (95% CI = 0.51–0.85) and for non-Asians was: 0.41 (95% CI = 0.17–0.64), respectively.

Conclusions:
The application of TRO surgery in ONFH can effectively relieve patients’ symptoms and they enjoy n a high survival rate, especially for Asian patients. This makes it a promising surgical technique.
The Impact of Bedside Cardiac Point-of-Care Ultrasound on the Utilization of Cardiology Subspecialty Resources in a Pediatric Emergency Department
Authors: Singh Amar , Starr-Seal Rebecca L. , Caperell Kerry S. , Warkentine Fred H. , Neal Ashley E. 
Published: November 2022

Objective
This study sought to determine the impact of cardiac point-of-care ultrasound (cPOCUS) in a pediatric emergency department (ED) on cardiology subspecialty utilization for subjects with chest pain or syncope. Diagnostic yield of cPOCUS and transthoracic echocardiograms (TTEs) for these subjects was also examined.

Methods
A retrospective chart review of subjects presenting to a tertiary pediatric ED with chest pain or syncope 1 year before (2015, pre-cPOCUS group) and 1 year after (2017, cPOCUS group) introduction of cPOCUS was conducted. Subjects aged 2 to 18 years evaluated for these symptoms were included. Those with known heart defects, prior abnormal TTE, or asthma exacerbation at presentation were excluded. In both groups, cardiology subspecialty utilization was assessed by determining whether cardiology referrals, cardiology consultations, or follow-up TTEs were completed. Results of TTEs were reviewed and classified as incidental (no follow-up needed), minor (follow-up needed, but intervention unlikely), moderate (nonurgent intervention needed), and severe (hospitalization/urgent intervention needed). Cardiac point-of-care ultrasound results were compared with any follow-up TTEs. Data were analyzed using χ2 or Student t test as appropriate.

Results
A total of 1230 subjects were analyzed: 595 pre-cPOCUS and 635 cPOCUS group. There was no significant difference in TTEs (42 vs 46), cardiology consultations (36 vs 37), or cardiology referrals (47 vs 37) between groups. Of 67 cPOCUS scans performed, 63 were normal, 3 showed small pericardial effusion, and 2 demonstrated left ventricular dysfunction. Of 88 TTEs in both groups (0.7% subjects), 76 were normal, 5 had incidental, 6 had minor, and 1 had a severe finding present on cPOCUS (0.08% subjects; 95% confidence interval, 0%–0.45%).

Conclusions
The introduction of cPOCUS did not increase cardiology subspecialty utilization in subjects presenting to the pediatric ED with chest pain or syncope. Cardiac point-of-care ultrasound may be useful in evaluating global biventricular systolic function and effusion in this population.
Point-of-Care Ultrasound and the Discovery of Pulmonary Arterial Hypertension in a Teenager
Authors: Assaf Raymen Rammy , Siembieda Joshua , Recto Michael R. , Doniger Stephanie J. 
Published: November 2022

Abstract
We present a case of a teenage patient with new-onset cardiac symptoms discovered to have primary pulmonary arterial hypertension. Point-of-care ultrasound used early in the patient's presentation identified significant right-sided heart dilatation and dysfunction despite the patient's relatively unrevealing physical examination. This article emphasizes the utility of performing focused cardiac ultrasound in pediatric patients early in their presentation. We briefly review focused cardiac ultrasound technique and highlight relevant literature.
Draft genome sequences for ten strains of Xanthomonas species that have phylogenomic importance
Authors: Harrison Jamie , Hussain Rana Muhammad Fraz , Greer Shannon , Ntoukakis Vardis , Aspin Andrew , Vicente Joana , Grant Murray , Studholme David John 
Published: November 2022

Here we report draft-quality genome sequences for pathotype strains of eight plant-pathogenic bacterial pathovars: Xanthomonas campestris pv. asclepiadis, X. campestris pv. cannae, X. campestris pv. esculenti, X. campestris pv. nigromaculans, X. campestris pv. parthenii, X. campestris pv. phormicola, and and X. campestris pv. zinniae. We also sequenced type strains of species X. melonis and X. dyei pv. eucalypti and unclassified Xanthomonas strain NCPPB 1067. These data will be useful for phylogenomic and taxonomic studies, filling some important gaps in sequence coverage of the Xanthomonas phylogenetic diversity. We include representatives of previously un-sequenced pathovars and species-level clades. Furthermore, these genome sequences may be useful in elucidating the molecular basis for important phenotypes such as biosynthesis of coronatine-related toxins and degradation of fungal toxin cercosporin.
A computational approach for measuring sentence information via surprisal: theoretical implications in nonfluent primary progressive aphasia
Authors: Rezaii Neguine , Michaelov James , Josephy-Hernandez Sylvia , Ren Boyu , Hochberg Daisy , Quimby Megan , Dickerson Bradford 
Published: November 2022

AbstractNonfluent aphasia is a language disorder characterized by simplified sentence structures as well as word-level abnormalities such as a reduced use of verbs and function words. According to the predominant account of the disorder, both structural and word-level features are caused by a core deficit in the processing of syntax. Under this account, however, it remains unclear why nonfluent patients choose semantically richer verbs and may have an intact comprehension of verbs and function words.Here, we propose and test the hypothesis that the word-level features of nonfluency reflect a process that selects lexically richer words to increase the information content of sentences.We use a computational linguistic method to measure the information content of sentences in the language of patients with nonfluent primary progressive aphasia (nfvPPA) (n = 36) and healthy controls (n = 133). We measure sentence information using surprisal, a metric calculated by the average probability of occurrence of words in a sentence given their preceding context.We found that by packaging their structurally simple sentences with lower frequency words, nfvPPA patients produce sentences with similar surprisal as that of healthy speakers. Furthermore, we found that higher sentence surprisal in nfvPPA correlates with a lower function-to-all-word ratio, a lower verb-to-noun ratio, and a higher heavy-to-all-verb ratio.Surprisal is an effective quantitative index of sentence information. Using surprisal allows for testing an account of nonfluent aphasia that regards word-level features of nonfluency as adaptive rather than defective symptoms, a finding that may entail revisions in therapeutic approaches to nonfluent speech.
Early, low-dose hydrocortisone and near-term brain connectivity in extremely preterm infants
Authors: Dubner Sarah E. , Rickerich Lucy , Bruckert Lisa , Poblaciones Rocío Velasco , Sproul Dawson , Scala Melissa , Feldman Heidi M. , Travis Katherine E. 
Published: November 2022

ABSTRACTBackgroundPostnatal steroids are used to prevent bronchopulmonary dysplasia in extremely preterm infants but may have adverse effects on brain development. This study assessed variation in connectivity metrics of major white matter pathways in the cerebrum and cerebellum at near-term gestational age among infants who did or did not receive a standardized regimen of hydrocortisone during the first 10 days of life.MethodsRetrospective cohort study. Participants: Infants born < 28 weeks, divided into 2 groups: Protocol group (n=33) received at least 50% of and not more than 150% of an intended standard dose of 0.5mg/kg hydrocortisone twice daily for 7 days, then 0.5 mg/kg per day for 3 days; versus Non-Protocol group (n=22), that did not receive protocol hydrocortisone or completed <50% of the protocol dose. We assessed group differences in near-term diffusion MRI mean fractional anisotropy (FA) and mean diffusivity (MD) across the corticospinal tract, inferior longitudinal fasciculus, corpus callosum and superior cerebellar peduncle.ResultsGroups were comparable in terms of gestational age, post-menstrual age at scan, medical complications, bronchopulmonary dysplasia, and necrotizing enterocolitis. No significant large-effect group differences were identified in mean FA or MD in any cerebral or cerebellar tract between the two groups.Conclusion(s)Low dose, early, postnatal hydrocortisone was not associated with significant differences in white matter tract microstructure at near term gestational age.ImpactThis study compared brain microstructural connectivity as a primary outcome among extremely preterm infants who did or did not receive early postnatal hydrocortisone.Low dose hydrocortisone in the first 10 days of life was not associated with significant differences in white matter microstructure in major cerebral and cerebellar pathwaysHydrocortisone did not have a significant effect on early brain white matter circuits.
Range of motion at baseline predict patient reported outcome measures in frozen shoulder patients treated with injections and rehabilitation
Authors: brindisino fabrizio , silvestri elena , Gallo Chiara , Di Giacomo Giovanni 
Published: November 2022

ABSTRACTFrozen shoulder is a common shoulder concern with a prevalence of 2-5 per cent in the general population that affects the shoulder joint between the ages of 40 and 60, mostly in female subjects, manifesting in progressive loss of glenohumeral movements associated with intense pain.The pathological process consists of a fibroproliferative tissue fibrosis and an inflammation of the synovial membrane. Although the pathophysiology of this condition has been deeply studied, the mechanisms underpinning remain poorly understood.Frozen shoulder manifests clinically as shoulder pain with progressive restricted movement, both active and passive, in the absence of trauma, along with normal radiographic scans of the glenohumeral joint.It classically progresses through 3 overlapping stages of pain (stage 1, lasting 2-9 months), stiffness (stage 2, lasting 4-12 months) and recovery (stage 3, lasting 5-24 months); however, up to day seems that pain-predominant and stiff-predominant phases could be more usefull in treatment modality choice and managing.The medical management has not been defined with a wide spectrum of operative and nonoperative treatments available. The most widely used treatments are local steroid and/or anesthetic injections, stretching, active and passive mobilization, physiotherapy, hydrodistension, capsular release; hoverver, he goals of the treatment are pain management, shoulder function restoration and improvement in quality of life. Based on the best available evidence it appears that the use of corticosteroid injections plus physiotherapy has been associated with better outcomes above all in terms of early benefit in ER ROM with clinical significance as long as 6 weeks of treatments.Nevertheless, it remains unclear which parameters influence the prognosis of the pathology.
Defining the causes of sporadic Parkinson’s disease in the global Parkinson’s genetics program (GP2)
Authors: Towns Clodagh , Richer Madeleine , Jasaityte Simona , Stafford Eleanor J. , Joubert Julie , Antar Tarek , Martinez-Carrasco Alejandro , Makarious Mary B. , Casey Bradford , Vitale Dan , Levine Kristin , Leonard Hampton , Pantazis Caroline B. , Screven Laurel A. , Hernandez Dena G. , Wegel Claire E. , Solle Justin , Nalls Mike A. , Blauwendraat Cornelis , Singleton Andrew B. , Tan Manuela M. X. , Iwaki Hirotaka , Morris Huw R. ,   the Global Parkinson’s Genetics Program (GP2)
Published: November 2022

AbstractThe Global Parkinson’s Genetics Program (GP2) will genotype over 150,000 participants from around the world, and integrate genetic and clinical data for use in large-scale analyses to dramatically expand our understanding of the genetic architecture of PD. This report details the workflow for cohort integration into the complex arm of GP2, and together with our outline of the monogenic hub in a companion paper, provides a generalizable blueprint for establishing large scale collaborative research consortia.