bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tenascin C regulates cancer cell glycolysis and tumor progression in prostate cancer
Authors: Qian Yongri , Liu Xingzhe , Feng Ying , Li Xiaogang , Xuan Yanhua 
Published: February 2022

ObjectivesTenascin C is a potential biomarker of cancer‐associated fibroblasts and has been significantly associated with poor prognosis in patients with prostate cancer. However, the effects of Tenascin C in prostate cancer cell glycolysis largely remain unclear. Thus, this study aimed to investigate the Tenascin C expression in prostate cancer and its correlation to glycolysis‐related protein and gene expression, clinicopathological parameters, and survival of patients.MethodsWe performed immunohistochemical staining for Tenascin C in 141 cases of primary prostate cancer. Based on public data sets, we explored the association of Tenascin C with angiogenesis‐related genes, M2 macrophage‐related gene, androgen receptor levels, PI3K/AKT/NF‐κB pathway genes, and glycolytic enzyme expression. The glucose uptake, lactate production, and glycolytic enzyme levels were detected by glycolysis assay and western blotting.ResultsOur results showed that Tenascin C expression is upregulated in prostate cancer tissues compared with benign prostatic hyperplasia tissues. High Tenascin C expression in prostate cancer cells was positively associated with lymph node metastasis, advanced clinical stage, the expression of CD105, CD206, and androgen receptor levels. The Kaplan–Meier curves showed a significant association of Tenascin C expression with the patient’s overall survival. Tenascin C expression was positively associated with PI3K p85, pAKT‐ser308, and NF‐κB p65 protein expression in prostate cancer samples. Moreover, siRNA‐mediated knockdown of Tenascin C expression inhibited cell glucose uptake, lactate production, and glycolytic‐enzyme expression in prostate cancer cells in vitro.ConclusionsTogether, our findings suggest that Tenascin C is a prognostic marker for patients with prostate cancer and that its effects might be mediated via regulation of the glycolysis process of prostate cancer cells.
Persistence of Tilapia tilapinevirus in fish rearing and environmental water and its ability to infect cell line
Authors: Yamkasem Jidapa , Prasartset Tharinthon , Tattiyapong Puntanat , Sirikanchana Kwanrawee , Mongkolsuk Skorn , Soto Esteban , Surachetpong Win 
Published: February 2022

AbstractTilapia tilapinevirus, or Tilapia Lake Virus (TiLV), is a RNA virus associated with mass morbidity and mortality in tilapia, leading to severe economic losses for global tilapia aquaculture. In this study, we investigated the persistence of TiLV in water by spiking sterile distilled water (SDW), freshwater collected from rearing fish tanks (FW) and natural pond water (PW) at 27°C as a representative of environmental water conditions with 0.6 ml of stock virus (3.18 × 107 viral copies/ml of water). The water samples were filtered through an electronegative charge membrane and quantified using reverse transcriptase quantitative PCR at 0, 3, 5, 7, 10 and 14 days post‐inoculation. The results revealed that TiLV RNA in SDW was reduced by 1.34 log10 in 14 days. A similar approximately 4 log10 removal of the virus in FW and PW was observed at 3 and 7 days, respectively. Moreover, the infectivity of TiLV was further studied; the virus lost its infectivity in E‐11 cells after 1 day in SDW, FW and PW water samples, even though the virus was spiked 10 more times than in the viral persistence study. Taken together, the results could be applied to improving biosecurity practices in tilapia farms by disinfecting or resting reservoir water for at least three to five days prior to stocking tilapia, to limit the spread of TiLV.
Money Illusion and TIPS Demand
Authors: LIOUI ABRAHAM , TARELLI ANDREA 
Published: February 2022

AbstractThe market demand for the Treasury Inflation‐Protected Securities (TIPS) is rather small. This is puzzling, as we show that an agent, who derives utility from real wealth and dynamically invests into multiple asset classes over a 30‐year horizon, incurs a certainty equivalent loss of 1.6% per annum from not investing in inflation‐indexed bonds. However, if the investor suffers from money illusion, the perceived loss is only 0.5% per annum. Furthermore, the perceived loss is totally negligible for an unsophisticated money‐illusioned investor ignoring the time variation of risk premia. Money illusion causes significant portfolio shifts from inflation‐indexed toward nominal bonds, with little effects on equity allocations, contributing to the low market demand for TIPS.
Chronic androgen excess in female mice does not impact luteinizing hormone pulse frequency or putative GABAergic inputs to GnRH neurons
Authors: Coyle Chris S. , Prescott Melanie , Handelsman David J , Walters Kirsty A. , Campbell Rebecca E. 
Published: February 2022

AbstractPolycystic ovary syndrome (PCOS) is associated with androgen excess and, frequently, hyperactive pulsatile luteinizing hormone (LH) secretion. Although the origins of PCOS are unclear, evidence from pre‐clinical models implicates androgen signalling in the brain in the development of PCOS pathophysiology. Chronic exposure of female mice to dihydrotestosterone (DHT) from 3 weeks of age drives both reproductive and metabolic impairments that are ameliorated by selective androgen receptor (AR) loss from the brain. This suggests centrally driven mechanisms in hyperandrogen‐mediated PCOS‐like pathophysiology that remain to be defined. Acute prenatal DHT exposure can also model the hyperandrogenism of PCOS, and this is accompanied by increased LH pulse frequency and increased GABAergic innervation of gonadotrophin‐releasing hormone (GnRH) neurons. We aimed to determine the impact of chronic exposure of female mice to DHT, which models the hyperandrogenism of PCOS, on pulsatile LH secretion and putative GABAergic input to GnRH neurons. To do this, GnRH‐green fluorescent protein (GFP) female mice received either DHT or blank capsules for 90 days from postnatal day 21 (n = 6 or 7 per group). Serial tail‐tip blood sampling was used to measure LH dynamics and perfusion‐fixed brains were collected and immunolabelled for vesicular GABA transporter (VGAT) to assess putative GABAergic terminals associated with GFP‐labelled GnRH neurons. As expected, chronic DHT resulted in acyclicity and significantly increased body weight. However, no differences in LH pulse frequency or the density of VGAT appositions to GnRH neurons were identified between ovary‐intact DHT‐treated females and controls. Chronic DHT exposure significantly increased the number of AR expressing cells in the hypothalamus, whereas oestrogen receptor α‐expressing neuron number was unchanged. Therefore, although chronic DHT exposure from 3 weeks of age increases AR expressing neurons in the brain, the GnRH neuronal network changes and hyperactive LH secretion associated with prenatal androgen excess are not evident. These findings suggest that unique central mechanisms are involved in the reproductive impairments driven by exposure to androgen excess at different developmental stages.
Effectiveness of multicomponent home‐based rehabilitation in older patients after hip fracture surgery: A systematic review and meta‐analysis
Authors: Lee Haneul , Lee Seon‐Heui 
Published: February 2022

AbstractBackgroundHip fracture is a major burden on health care systems worldwide and requires hospitalisation for a long period.ObjectiveThe aim was to evaluate the effectiveness of multicomponent home‐based rehabilitation compared with different control interventions (in‐hospital rehabilitation, active control or usual care) in older patients after hip fracture surgery.DesignSystematic review and meta‐analysis of randomised controlled studies.Data sourcesWe searched three electronic databases, including Ovid‐MEDLINE, Ovid‐Embase, CINAHL and the Cochrane Library for relevant articles up to March 2020.Review methodsTwo investigators independently extracted data and assessed study quality using the risk of bias. Data were analysed using Review Manager 5.3. The current review employs the PRISMA procedure.ResultsOut of 2996 studies, 22 articles were relevant for this review and meta‐analysis. Among them, five compared the multicomponent home‐based rehabilitation with in‐hospital rehabilitation, one compared it with active control, six with usual care and ten compared the home exercise only with usual care. There was no significant difference in activities of daily living (ADL) between multicomponent home‐based rehabilitation and in‐hospital rehabilitation, while multicomponent home‐based rehabilitation significantly increased in ADL when compared to usual care. Home exercises also had significant effects on ADL, quality of life(QoL), balance, gait and muscle strength of the knee extensor compared with usual care (p < .05).ConclusionsMulticomponent home‐based rehabilitation is comparable to in‐hospital rehabilitation regarding improvements in muscle strength, gait speed, balance, ADL and QoL.Relevant to clinical practiceMulticomponent home‐based rehabilitation is comparable to in‐hospital rehabilitation regarding improvements in muscle strength, gait speed, balance, ADL and QoL. Also, high adherence to home exercise may be associated with better clinical outcomes. Therefore, more compliance‐oriented multicomponent home‐based rehabilitation programmes for older patients after hip fracture must be developed by health care professionals, including physical therapist, to ensure optimum home‐based rehabilitation.
Risk stratification for early and late falls in acute care settings
Authors: Satoh Masae , Miura Takeshi , Shimada Tomoko , Hamazaki Toyoko 
Published: February 2022

AbstractBackground and AimsFalling generally injures patients, lengthens hospital stays and leads to the wastage of financial and medical resources. Although falls can occur at any stage after hospital admission, there are no studies that characterise falls with length of hospital stay in acute care settings. This study aims to clarify risk stratification of early and late falls in acute care settings.MethodsA retrospective cohort study was conducted for participants who were admitted to a teaching hospital in Japan. Patients’ falls were divided into two groups based on the median of the fall date (day 10). Considering a 70/30 split, the logistic regression model was used to extract independent predictors for early and late falls for nine risk variables based on exploratory analysis among 26 items selected from the modified Japanese Nursing Association Fall Risk Assessment Tool, and risk models were validated. This study was conducted according to the STROBE guideline.ResultsOf the 10,975 patients admitted, 87 and 90 with early and late falls, respectively, were identified. The five significant risk factors extracted for early falls were fall history, muscle weakness, impaired understanding, use of psychotropics and the personality trait of ‘doing everything on one's own’; risk factors identified for late falls were being older than 65 years, impaired extremities and unstable gait, in addition to muscle weakness. Using these variables for early and late falls in the validation cohort, the concordance indices of the risk models were both over 0.80.ConclusionsBy separately extracting risk factors for early and late falls in an acute care hospital setting, this study shed light on the characteristics of the respective types of falls.Relevant to clinical practiceAs the risk factors of falls vary according to the length of hospitalisation, specific preventive care can be implemented to avoid fall incidents.
Today's challenge may be tomorrow's hindrance (and vice versa): Longitudinal changes in employee’s appraisals of job demands and their outcomes
Authors: Li Peikai , Taris Toon W. , Peeters Maria C. W. 
Published: February 2022

Researchers have long been interested in understanding how appraisals influence stressor–outcome relationships. Most studies in this area employ a variable‐centred approach, which ignores the possibility that there may be subpopulations of employees who differ in the combined use of challenge and hindrance appraisals. Building on transactional stress theory, we investigated (a) the potential existence of distinct latent appraisals profiles of job demands (i.e. time urgency, role conflict and emotional demands), (b) the outcomes associated with particular appraisal profiles and (c) the stability of these profiles over time. In a two‐wave study with a one‐year time interval (T1, N = 535, T2, N = 152) among Chinese workers, we identified three distinct appraisals profiles in both study waves (i.e. ‘positivists’, ‘negativists’ and ‘indifferent workers’). The positivists reported the highest levels of engagement, job satisfaction and the lowest levels of burnout. Interestingly, most participants appeared to change their appraisal profile over time (i.e. very often from ‘negativist’ and ‘positivist’ to ‘indifferent worker’, while they were less likely to change their appraisal profile to ‘positivist’). Furthermore, job demands influenced employees' appraisal profiles. Taken together, our results shed light on the nature of the appraisal of demands in the work context and how different employees use distinct combinations of appraisal to address their work demands.Practitioner points

Managers should be aware that there are subgroups of employees that appraise their job demands differently: positivists, negativists and indifferent workers. Positivists tend to have higher well‐being than negativists and indifferent workers.

Employees appear to change their appraisals of job demands over time. In particular, positivists tend to become indifferent workers. It is important that organizations provide sufficient resources and support to their employees to promote high challenge appraisals.
Waxy bloom on capsules is a major determinant of early infection by gray mold (Amphobotrys ricini(N.F. Buchw.) Hennebert) in castor (Ricinus communisL.)
Authors: Ayesha Parveen Pattan , Venkata Ramana Jagarlamudi , Durga Prasad Ravulapalli , Senthilvel Senapathy , Lal Ahamed Muhammad , Greeshma Kothur , Dinesh Kumar Viswanathaswamy , Sarada Chunduri 
Published: February 2022

AbstractGray mold is a major disease of castor caused by the pathogenAmphobotrys ricini. It mainly affects the inflorescence resulting in severe yield losses. Role of qualitative characters of inflorescence (spike) and capsuleviz. spike compactness, capsule type, waxy bloom intensity on capsules (visual), capsule spine length, spine texture and spine density in predisposing castor crop to gray mold was investigated. A set of 26 castor lines differing for presence and intensity of bloom was characterized for spike and capsule characters. The lines were screened in vitro (detached spike and capsule techniques) and in vivo by raising the plants in poly house and field conditions for disease reaction. Spearman's rank correlation analysis revealed significant positive correlation (rs = >0.5;p = <0.05) between disease severity and capsule type, capsule waxy bloom, spine texture, spine length. Principal component regression analysis revealed that waxy bloom intensity is the major factor, among the evaluated, that predisposes castor lines to the fungus infection promoting disease severity. Quantification of epicuticular wax from capsules of different waxy bloom lines revealed that there was an increase in severity of infection with increase in wax quantity on capsules irrespective of nature of bloom substantiating the role of waxy bloom quantity in promoting the infection. High quantities of wax (1.50–3.00 µg/mg) were extracted from lines showing high per cent disease severity (40%–95%), and low quantities of wax (0.00–1.50 µg/mg) were extracted from lines showing low per cent disease severity (1%–40%). A disease scoring scale considering differences in waxy bloom quantity as a biochemical marker has been proposed. It may not be a replacement to phenotypic screenings but helps breeders in selecting early generation material for low disease severity when huge populations are to be screened against gray mold by choosing low waxy bloom varieties.
Does Enthusiasm for Work Lead to Typical Burnout? A Three‐Wave Panel Study with Caregivers1, 2
Authors: Igawa Junichi , Fukuzaki Toshiki , Iotake Ryosuke , Nakanishi Daisuke 
Published: February 2022

AbstractPrevious studies have indicated that burnout arises from excessive enthusiasm for work. However, most psychological scale items that measure burnout focus solely on symptoms, and there is little evidence to indicate whether burnout symptoms can derive from enthusiasm for work. Therefore, we conducted a three‐wave annual panel survey to assess whether scores on the Burnout Scale (BO scores), which focuses solely on symptoms, measure “typical burnout” caused by enthusiasm for work among Japanese caregivers. We used a cross‐lagged and synchronous effects model and found that enthusiasm indicators negatively predicted BO scores. This result contradicts the assumptions of typical burnout. Subsequently, we classified enthusiasm indicators and BO scores through latent rank analysis. The results showed that people with high enthusiasm had low BO scores, and vice versa, indicating that very few people have typical burnout characteristics, and that the scale focuses only on symptoms and cannot measure typical burnout. Future studies should investigate the mechanism of typical burnout to distinguish between burnout and similarly presenting diagnoses.
Renal biomarkers in cats: A review of the current status in chronic kidney disease
Authors: Kongtasai Thirawut , Paepe Dominique , Meyer Evelyne , Mortier Femke , Marynissen Sofie , Stammeleer Lisa , Defauw Pieter , Daminet Sylvie 
Published: February 2022

AbstractSerum creatinine concentration, the classical biomarker of chronic kidney disease (CKD) in cats, has important limitations that decrease its value as a biomarker of early CKD. Recently, serum symmetric dimethylarginine concentration was introduced as a novel glomerular filtration rate biomarker for the early detection of CKD in cats. However, data on its specificity are still limited. The limitations of conventional biomarkers and the desire for early therapeutic intervention in cats with CKD to improve outcomes have prompted the discovery and validation of novel renal biomarkers to detect glomerular or tubular dysfunction. Changes in the serum or urinary concentrations of these biomarkers may indicate early kidney damage or predict the progression of kidney before changes in conventional biomarkers are detectable. This review summarizes current knowledge on renal biomarkers in CKD in cats, a field that has progressed substantially over the last 5 years.
A novel bactericidal small molecule, STK-35, and its derivative, STK-66, as antibacterial agents against Gram-negative pathogenic bacteria in vitro and in vivo
Authors: She Pengfei , Xu Lanlan , Liu Yaqian , Liu Shasha , Li Zehao , Li Yimin , Hussain Zubair , Wu Yong 
Published: February 2022

Abstract
Due to the increasing rate of antibiotic resistance and the emergence of persister cells of Gram-negative pathogenic bacteria, the development of new antibacterial agents is urgently needed to deal with this problem. Our results indicated that both newly identified small molecule STK-35 and its derivative STK-66 exhibited effective antibacterial properties against a variety of Gram-negative pathogens including Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. The minimal inhibitory concentrations and minimal bactericidal concentrations ranges were 0·0625–8 μg ml−1 and 0·125–16 μg ml−1, respectively, while no haemolytic activity and mammalian cell cytotoxicity were observed. The time-killing assays showed STK-35/66 had strong bactericidal activity against Gram-negative pathogens. STK-35/66 also showed different degrees of synergistic antibacterial activity with conventional antibiotics and exhibited persister cells killing activity. Moreover, STK-35/66 effectively eradicated the pre-formed biofilms of P. aeruginosa and A. baumannii. In addition, STK-35/66 significantly increased the survival rate of E. coli infected mice and induced a decrease in bacterial load of the peritonitis model. In nutshell, these results suggested that STK-35/66 possessed antimicrobial activity against Gram-negative pathogenic bacteria in vitro and in vivo, which could be considered as potential substitutes for the treatment of Gram-negative pathogenic infections after further structure optimization.
The origin of the potassium‐rich annular zones at the Bosumtwi impact structure, Ghana, investigated by field study, radiometric analysis, and first cosmogenic nuclide data
Authors: Niang Cheikh Ahmadou Bamba , Baratoux David , Rochette Pierre , Braucher Regis , Reimold Wolf Uwe , Lambert Philippe , Diallo Dinna Pathe , Regard Vincent , Carretier Sebastien , Jessell Mark W. , Faye Gayane , Koeberl Christian 
Published: February 2022

AbstractThe 10.5‐km‐diameter, 1 Ma Bosumtwi impact structure in Ghana is one of the youngest, large impact structures known on Earth. The preservation of the morphology of its ejecta deposits, with an annular moat and outer ridge resembling those of rampart impact craters on Mars, makes Bosumtwi a remarkable impact structure on the African continent. An airborne radiometric survey of the southwestern part of Ghana reveals enigmatic circular feature enriched in potassium, coinciding with the crater rim and an outer ejecta ridge at Bosumtwi. The goal of this study is to investigate possible origins of these features, by impact processes (shock metamorphic effects, impact‐induced hydrothermal systems) or postimpact surficial processes (erosion, weathering). The origin of these features is discussed here based on field observations, ground‐based radiometric measurements, and first cosmogenic nuclide analyses (10Be). The data indicate that the rim and outer ridge were eroded more rapidly than the rest of the impact structure. Accordingly, the downward advance of the weathering fronts in the annular moat, after ejecta emplacement, are responsible for leaching of K from the lateritic residual observed at the surface. The Bosumtwi impact structure is, therefore, a valuable natural laboratory to investigate the factors controlling erosion and weathering processes in the Ashanti belt since impact 1 Ma ago. Simulations of vertical profiles of 10Be concentration further constrain local variations of the erosion rate. In light of this study, circular K anomalies in radiometric surveys might be indicative of potential impact structures in tropical regions.