bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of lncRNA MIR155HG-modified MSCs and exosome delivery to synergistically attenuate vein graft intimal hyperplasia
Authors: Bai Xiao , Qi Zaiwen , Zhu Mingzhen , Lu Zhuangzhuang , Zhao Xin , Zhang Lining , Song Guangmin 
Published: November 2022

Abstract
Background
The mesenchymal stem cells (MSCs) were used to repair tissue injury. However, the treatment effect was not satisfactory. We investigated whether lncRNA MIR155HG could promote survival and migration of MSCs under oxidative stress, which mimics in vivo environments. Furthermore, we studied the protective effect of exosomes secreted by MSCs transfected with MIR155HG on endothelial cells. This study aimed to determine whether exploiting MSCs and exosomes modified with lncRNA MIR155HG would exert synergistic therapeutic effect to attenuate vein graft intimal hyperplasia more effectively.

Methods
Lentivirus containing lncRNA MIR155HG overexpressing vector was packaged and used to infect MSCs. Then, CCK-8 assay, flow cytometry, Transwell assay, and Elisa assay were used to assess the functional changes of MSCs with overexpressed MIR155HG (OE-MSCs). Furthermore, the associated pathways were screened by Western blot. MIR155HG-MSCs-derived exosomes (OE-exo) were collected and co-cultured with human umbilicus vein endothelial cell (HUVEC). We validated the protective effect of OE-exo on HUVEC. In vivo, both MSCs and exosomes modified with MIR155HG were injected into a vein graft rat model via tail vein. We observed MSCs homing and intimal hyperplasia of vein graft using a fluorescent microscope and histological stain.

Results
Our study found that lncRNA MIR155HG promoted proliferation, migration, and anti-apoptosis of MSCs. NF-κB pathway took part in the regulation process induced by MIR155HG. OE-exo could enhance the activity and healing ability of HUVEC and reduce apoptosis. In vivo, OE-MSCs had a higher rate of homing to vascular endothelium. The combined treatment with OE-MSCs and OE-exo protected vascular endothelial integrity, reduced inflammatory cell proliferation, and significantly attenuated intimal hyperplasia of vein graft.

Conclusion
LncRNA MIR155HG could promote the survival and activity of MSCs, and reduce the apoptosis of HUVECs using exosome delivery. Exploiting MSCs and exosomes modified with MIR155HG could attenuate vein graft intimal hyperplasia more effectively and maximize the surgical effect.
Chromosomal and gonadal sex drive sex differences in lipids and hepatic gene expression in response to hypercholesterolemia and statin treatment
Authors: Wiese Carrie B. , Agle Zoey W. , Zhang Peixiang , Reue Karen 
Published: November 2022

Abstract
Background
Biological sex impacts susceptibility and presentation of cardiovascular disease, which remains the leading cause of death for both sexes. To reduce cardiovascular disease risk, statin drugs are commonly prescribed to reduce circulating cholesterol levels through inhibition of cholesterol synthesis. The effectiveness of statin therapy differs between individuals with a sex bias in the frequency of adverse effects. Limited information is available regarding the mechanisms driving sex-specific responses to hypercholesterolemia or statin treatment.

Methods
Four Core Genotypes mice (XX and XY mice with ovaries and XX and XY mice with testes) on a hypercholesteremic Apoe–/– background were fed a chow diet without or with simvastatin for 8 weeks. Plasma lipid levels were quantified and hepatic differential gene expression was evaluated with RNA-sequencing to identify the independent effects of gonadal and chromosomal sex.

Results
In a hypercholesterolemic state, gonadal sex influenced the expression levels of more than 3000 genes, and chromosomal sex impacted expression of nearly 1400 genes, which were distributed across all autosomes as well as the sex chromosomes. Gonadal sex uniquely influenced the expression of ER stress response genes, whereas chromosomal and gonadal sex influenced fatty acid metabolism gene expression in hypercholesterolemic mice. Sex-specific effects on gene regulation in response to statin treatment included a compensatory upregulation of cholesterol biosynthetic gene expression in mice with XY chromosome complement, regardless of presence of ovaries or testes.

Conclusion
Gonadal and chromosomal sex have independent effects on the hepatic transcriptome to influence different cellular pathways in a hypercholesterolemic environment. Furthermore, chromosomal sex in particular impacted the cellular response to statin treatment. An improved understanding of how gonadal and chromosomal sex influence cellular response to disease conditions and in response to drug treatment is critical to optimize disease management for all individuals.
Salidroside reduces neuropathology in Alzheimer’s disease models by targeting NRF2/SIRT3 pathway
Authors: Yao Yuyuan , Ren Zhichu , Yang Ruihan , Mei Yilan , Dai Yuying , Cheng Qian , Xu Chong , Xu Xiaogang , Wang Sanying , Kim Kyoung Mi , Noh Ji Heon , Zhu Jian , Zhao Ningwei , Liu Yong U. , Mao Genxiang , Sima Jian 
Published: November 2022

AbstractBackgroundNeurite dystrophy is a pathologic hallmark of Alzheimer’s disease (AD). However, drug discovery targeting neurite protection in AD remains largely unexplored.MethodsAβ-induced neurite and mitochondrial damage assays were used to evaluate Aβ toxicity and the neuroprotective efficacy of a natural compound salidroside (SAL). The 5×FAD transgenic mouse model of AD was used to study the neuroprotective function of SAL. To verify the direct target of SAL, we used surface plasmon resonance and cellular thermal shift assays to analyze the drug-protein interaction.ResultsSAL ameliorates Aβ-mediated neurite damage in cell culture. We further reveal that SAL represses mitochondrial damage in neurites by promoting mitophagy and maintaining mitochondrial homeostasis, dependent on an NAD-dependent deacetylase SIRT3. In AD mice, SAL protects neurite morphology, mitigates Aβ pathology, and improves cognitive function, which are all SIRT3-dependent. Notably, SAL directly binds to transcription factor NRF2, inhibits its degradation by blocking its interaction with KEAP1 ubiquitin ligase, and then advances NRF2-mediated SIRT3 transcription.ConclusionsOverall, we demonstrate that SAL, a potential anti-aging drug candidate, attenuates AD pathology by targeting NRF2/SIRT3 pathway for mitochondrial and neurite protection. Drug discovery strategies focusing on SAL may thus provide promising therapeutics for AD.
Scattering for 2D quasilinear wave equations with null conditions
Authors: Sun Yaqing , He Daoyin 
Published: November 2022

AbstractWe study the scattering theory of solutions to the quasilinear wave equations with null conditions and small initial data in two dimensions. Based on the scattering profile that was described precisely in He–Liu–Wang (J. Differ. Equ. 269(4):3067–3088, 2020), we establish precise estimates for the difference of the solution and the scattering profile. Therefore, collecting the results in this paper and those in He–Liu–Wang (J. Differ. Equ. 269(4):3067–3088, 2020), we have given a basically systematic study on the long-time behavior of a small data solution to the 2D wave equation with null conditions.
Land use land cover change in and around Chebera Churchura National Park, Southwestern Ethiopia: implications for management effectiveness
Authors: Yadeta Temesgen , Tessema Zewdu Kelkay , Kebede Fanuel , Mengesha Girma , Asefa Addisu 
Published: November 2022

Abstract
Background
Land use-land cover change (LULCC) resulting from anthropogenic activities has been one of the major causes of loss of biodiversity globally. Thus, understanding the dynamics of such LULCC, especially within protected areas, is important to assess their past-present management effectiveness, identify the driving forces and assist future implementation of mitigation measures. The objectives of this study, based on satellite images analysis, were to examine and describe patterns of LULCC within and in the buffer zone area of Chebera Churchura National Park, SW Ethiopia, at five points in time (1985, 1991, 2000, 2010, and 2020); and to examine management effectiveness of the park authority in slowing down the rate of LULCCs after the establishment of the park.

Results
Results showed that different land cover types exhibited varying patterns of change over the last 35 years. However, major differences were detected between the park area boundary and its buffer zone area after establishment of the park. From the year 1991 to 2000 (before the establishment), grassland declined by 52.9% (120.55km2) and 58.9% (151.9km2) within the park boundary and its buffer zone, respectively. After the establishment, grassland continued to decline only in the buffer zone. Similarly, agricultural land declined by 13.2% (2.7 km2) within the park but expanded by 5.4% (3.1 km2) in the buffer zone prior to park establishment. However, after establishment of the park, agricultural land in the buffer zone increased by 99.4% (99.7 km2). Grassland and wooded grassland had the opposite trend within the park area before vs after the establishment of the park.

Conclusions
Results of the present study showed that striking differences in the rates of LULCCs within the park area and its buffer zone after establishment of the park, with the implication that the management is effective in slowing down LULCC relative to that in the buffer zone areas. In sum, this study would serve as a showcase on cost-effective means to assess human impacts on biodiversity in protected areas due to policy changes. Findings of the study would serve as a bench mark to evaluate future management effectives of the park authority.
Maternal organic selenium supplementation during gestation enhances muscle fiber area and muscle fiber maturation of offspring in porcine model
Authors: Lin Yan , Yan Hui , Cao Lei , Mou Daolin , Ding Dajiang , Qin Binting , Che Lianqiang , Fang Zhengfeng , Xu Shengyu , Zhuo Yong , Li Jian , Wang Jianping , Huang Chao , Zou Yuanfeng , Li Lixia , Wu De , Feng Bin 
Published: November 2022

Abstract
Background
Organic selenium supplementation during gestation improves the antioxidant status and reproductive performance of sows and increases the antioxidative capacity of the intestines of their offspring. This study was conducted to investigate the effect of maternal basel diet (control) supplemented with an organic Se, 2-hydroxy-4-methylselenobutanoic acid (HMSeBA), or inorganic sodium selenite (Na2SeO3) during gestation on the antioxidant status and development of muscle in newborn and weaned piglets. Newborn piglets before colostrum intake and weaned piglets were selected for longissimus dorsi (LD) muscle collection and analysis.

Results
The results showed that maternal HMSeBA supplementation increased the muscle area and content of Se in the LD muscle of newborn piglets, improved gene expression of selenoproteins, and decreased oxidative status in the LD muscle of both newborn and weaned piglets compared with the control. The expression of muscle development-related genes of newborn piglets in the HMSeBA group was lower than in the control group, whereas the expression of MRF4 in weaned piglets was higher in the HMSeBA group than in the control and Na2SeO3 groups. In addition, HMSeBA supplementation decreased the mRNA expressions of myosin heavy chains (MyHC) IIx and MyHC IIb and the percentage of MyHC IIb; increased the expression of PGC-1α in the LD muscle of newborn piglets; increased the gene expression of MyHC IIa; and decreased the protein expression of slow MyHC and the activity of malate dehydrogenase in the LD muscle of weaned piglets compared with the control group.

Conclusions
Maternal HMSeBA supplementation during gestation can improve the antioxidative capacity of the muscle of their offspring and promote the maturity of muscle fibres in weaned offspring.
The effect of COVID-19 home quarantine on the psychological state of pharmacy students: a cross-sectional study
Authors: Mohamed Asmaa S. , Abdelrahman Ahmed A. , Ahmad Hosam M. , Abdel Aziz Omar R. , Mesameh Yasmeen S. , Mohamad Soad A. 
Published: November 2022

AbstractBackgroundPsychological morbidity has been documented in medical and pharmaceutical undergraduate students in different countries around the world. In this study, we examined the impact of coronavirus disease 2019 (COVID-19) home quarantine on the depressive psychological aspects of last-grade pharmacy students.MethodsA cross-sectional study was conducted by the Department of Clinical Pharmacy, Faculty of Pharmacy, Deraya University, Egypt. Two hundred and sixty-eight last-grade pharmacy students were included in this study, and they completed a self-administered, pre-designed, anonymous questionnaire. The main outcome measures were the Hamilton Depression Rating Scale (HRS) and Patient Health Questionnaire-9 (PHQ-9), which were measured to screen for the symptoms of psychological depression and determine the degree of depression severity between the beginning and the end of the COVID-19 home quarantine period. Data entry and analysis were done using the Statistical Package for Social Science (SPSS) software version 26. Descriptive statistics were employed for analyses of the data, and categorical variables were described by frequencies and percentages. Bivariate and multivariable analyses were performed to examine relations between demographic data and psychological scales. The study protocol was approved by the Faculty of Pharmacy, Minia University Ethical Committee.ResultsA total of 268 students participated in this study (102 males and 166 females). The mean ± SD score of baseline HRS and HRS at the end of the study was 6.3 ± 4.45, 7.95 ± 5.36, respectively, with the presence of a statistically significant difference between the two scores (p < 0.001). The mean ± SD score of baseline PHQ-9 and PHQ-9 at the end of the study was 4.35 ± 3.45, 5.37 ± 4.14, respectively, with the presence of a statistically significant difference between the two scores (p < 0.001). The results showed that the COVID-19 home quarantine period led to a depressive psychological effect on the students in this study.ConclusionsStudents’ psychological depression causes morbidity and, in some cases, mortality. Psychological depressive problems were significantly associated with the COVID-19 home quarantine period, which calls for early intervention to solve it. Student counselling services must be more accessible and affordable to overcome this problem.
Quantitative CT analysis to predict esophageal fistula in patients with advanced esophageal cancer treated by chemotherapy or chemoradiotherapy
Authors: Shi Yan-Jie , Liu Chang , Wei Yi-Yuan , Li Xiao-Ting , Shen Lin , Lu Zhi-Hao , Sun Ying-Shi 
Published: November 2022

Abstract
Background
Esophageal fistula is one of the most serious complications of chemotherapy or chemoradiotherapy (CRT) for advanced esophageal cancer. This study aimed to evaluate the performance of quantitative computed tomography (CT) analysis and to establish a practical imaging model for predicting esophageal fistula in esophageal cancer patients treated with chemotherapy or chemoradiotherapy.

Methods
This study retrospectively enrolled 204 esophageal cancer patients (54 patients with fistula, 150 patients without fistula) and all patients were allocated to the primary and validation cohorts according to the time of inclusion in a 1:1 ratio. Ulcer depth, tumor thickness and length, and minimum and maximum enhanced CT values of esophageal cancer were measured in pretreatment CT imaging. Logistic regression analysis was used to evaluate the associations of CT quantitative measurements with esophageal fistula. Receiver operating characteristic curve (ROC) analysis was also used.

Results
Logistic regression analysis showed that independent predictors of esophageal fistula included tumor thickness [odds ratio (OR) = 1.167; p = 0.037], the ratio of ulcer depth to adjacent tumor thickness (OR = 164.947; p < 0.001), and the ratio of minimum to maximum enhanced CT value (OR = 0.006; p = 0.039) in the primary cohort at baseline CT imaging. These predictors were used to establish a predictive model for predicting esophageal fistula, with areas under the receiver operating characteristic curves (AUCs) of 0.946 and 0.841 in the primary and validation cohorts, respectively. The quantitative analysis combined with T stage for predicting esophageal fistula had AUCs of 0.953 and 0.917 in primary and validation cohorts, respectively.

Conclusion
Quantitative pretreatment CT analysis has excellent performance for predicting fistula formation in esophageal cancer patients who treated by chemotherapy or chemoradiotherapy.
A Simple Model for Diagnosis of Maladaptations to Exercise Training
Authors: Flockhart Mikael , Nilsson Lina C. , Ekblom Björn , Larsen Filip J. 
Published: November 2022

Abstract
Background
The concept of overreaching and super compensation is widely in use by athletes and coaches seeking to maximize performance and adaptations to exercise training. The physiological aspects of acute fatigue, overreaching and non-functional overreaching are, however, not well understood, and well-defined negative physiological outcomes are missing. Instead, the concept relies heavily on performance outcomes for differentiating between the states. Recent advancements in the field of integrated exercise physiology have associated maladaptations in muscular oxidative function to high loads of exercise training.

Method
Eleven female and male subjects that exercised regularly but did not engage in high-intensity interval training (HIIT) were recruited to a 4-week long training intervention where the responses to different training loads were studied. Highly monitored HIIT sessions were performed on a cycle ergometer in a progressive fashion with the intent to accomplish a training overload. Throughout the intervention, physiological and psychological responses to HIIT were assessed, and the results were used to construct a diagnostic model that could indicate maladaptations during excessive training loads.

Results
We here use mitochondrial function as an early marker of excessive training loads and show the dynamic responses of several physiological and psychological measurements during different training loads. During HIIT, a loss of mitochondrial function was associated with reduced glycolytic, glucoregulatory and heart rate responses and increased ratings of perceived exertion in relation to several physiological measurements. The profile of mood states was highly affected after excessive training loads, whereas performance staled rather than decreased. By implementing five of the most affected and relevant measured parameters in a diagnostic model, we could successfully, and in all the subjects, identify the training loads that lead to maladaptations.

Conclusions
As mitochondrial parameters cannot be assessed without donating a muscle biopsy, this test can be used by coaches and exercise physiologists to monitor adaptation to exercise training for improving performance and optimizing the health benefits of exercise.
Clinical trial registry numberNCT04753021. Retrospectively registered 2021-02-12.
Emotional interdependence: the key to studying extrinsic emotion regulation
Authors: Kinkead Ana , Salas Riquelme Christian 
Published: November 2022

Abstract
The literature on extrinsic emotion regulation or the intention to modify other people’s emotions has grown in recent years, accompanied by proposals in which its definition is made more precise, the way to understand it in relation to other related processes is delimited, and the consequences of its use in the quality of close relationships are evidenced. Conceptual reviews on this topic recognize the importance of examining the affect and dyadic dynamics that arise between those who regulate each other extrinsically. This dynamic refers to emotional interdependence, the potential of the members of a dyad to shape each other’s emotions reciprocally, particularly in those who share a close bond, such as that of a romantic couple. There is little theoretical development regarding the relevance of this characteristic in relation to EER. This article has two objectives: (1) to make a narrative synthesis of the characteristics that define EER and (2) to expand and complexify the existing model by including the emotional interdependence as a vital component in the understanding of the functioning of EER. Lastly, the role of emotional interdependence in the emergence, maintenance, and satisfaction concerning couple relationships is made explicit through phenomena such as shared reality.
Efficacy of the entomopathogenic fungus, Beauveria bassiana and Lecanicillium muscarium against two main pests, Bemisia tabaci (Genn.) and Tetranychus urticae (Koch), under geothermal greenhouses of Southern Tunisia
Authors: Chouikhi Sabrine , Assadi Besma Hamrouni , Lebdi Kaouthar Grissa , Belkadhi Mohamed Sadok 
Published: November 2022

Abstract
Background
The geothermal greenhouses in Southern Tunisia are an important axis of agricultural development. This sector faces many abiotic and biotic constraints that could threat its sustainability. Thus, the heated greenhouses encounter destructive pests such as the whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) and the two-spotted spider mite Tetranychus urticae (Koch) (Acari: Tetranychidae).

Results
This study aimed to assess the effect of the entomopathogenic fungi (EPF) Beauveria bassiana (strain ATCC and strain R444) and Lecanicillium muscarium strain Ve6 on simultaneous existence of T. urticae and B. tabaci in the host plants. The EPF had a significant effect on eggs and larvae of B. tabaci and on eggs and mobile forms of T. urticae in particular. The use of B. bassiana ATCC, B. bassiana R444 and L. muscarium strains Ve6 showed significant efficacies against B. tabaci larvae and eggs compared to untreated control. Indeed, the reduction percent of B. tabaci eggs varied between 42.65 and 58.52%. Thus, the efficacy against the number of B. tabaci larvae was in order to 65.04, 60.26 and 55.52% of B. bassiana strain ATCC, B. bassiana strain R444 and L. muscarium strain Ve6, respectively. In addition, these EPF were very effective on T. urticae eggs with a percentage reduction greater than 92.86%, whereas the percentage reduction in the T. urticae mobile forms varied between 95.11 and 98.52%.

Conclusions
Use of EPF will be an imperative to develop directed interventions at the integrated management of these two pests in protected and geothermal crops.
Computational spectropolarimetry with a tunable liquid crystal metasurface
Authors: Ni Yibo , Chen Chen , Wen Shun , Xue Xinyuan , Sun Liqun , Yang Yuanmu 
Published: November 2022

AbstractWhile conventional photodetectors can only measure light intensity, the vectorial light field contains much richer information, including polarization and spectrum, that are essential for numerous applications ranging from imaging to telecommunication. However, the simultaneous measurement of multi-dimensional light field information typically requires the multiplexing of dispersive or polarization-selective elements, leading to excessive system complexity. Here, we demonstrate a near-infrared spectropolarimeter based on an electrically-tunable liquid crystal metasurface. The tunable metasurface, which acts as an encoder of the vectorial light field, is tailored to support high-quality-factor guided-mode resonances with diverse and anisotropic spectral features, thus allowing the full Stokes parameters and the spectrum of the incident light to be computationally reconstructed with high fidelity. The concept of using a tunable metasurface for multi-dimensional light field encoding may open up new horizons for developing vectorial light field sensors with minimized size, weight, cost, and complexity.