bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Volatile Profiles and DNA Barcodes of Lauraceae Species from the Ocotea Complex with Occurrence in the Brazilian Amazon
Authors: Xavier Júlia Karla A. M. , da Trindade Rafaela C. S. , Cibelle Moreira Edith , Figueiredo Pablo Luis B. , Maia José Guilherme S. , Setzer William N. , da Silva Joyce Kelly R. 
Published: September 2022

AbstractThe Ocotea complex accommodates most of the taxonomic diversity of Neotropical Lauraceae with economic importance and biological potential attributed to their essential oils (EOs) and extracts. However, the botanical taxonomy has had limitations due to the difficulty of identifying and delimiting species and genera. The chemical and molecular markers of Ocotea complex species in Pará state, Brazil, were assessed according to their EO compositions and DNA sequences of matK, trnL‐trnF, and ITS regions. The multivariate analysis of EOs constituents has classified them into two main clusters characterized by oils rich in (I) terpenoids and phenylpropanoids and (II) sesquiterpenes. We conducted a phylogenetic analysis of species based on DNA barcode sequences on the Bayesian Inference (PP: 0.70–1,0) and Maximum Likelihood (BS: 72–100 %). The comparison between the volatile profiles and phylogenetic data indicates two main groups for these species collected from the Ocotea complex.
Recent Advances of Curcumin Derivatives in Breast Cancer
Authors: Yin Ying , Tan Yan , Wei Xueni , Li Xiaoshun , Chen Hongfei , Yang Zehua , Tang Guotao , Yao Xu , Mi Pengbing , Zheng Xing 
Published: September 2022

AbstractCurcumin is a potential plant‐derived drug for the treatment of breast cancer. Poor solubility and bioavailability are the main factors that limit its clinical application. Various structural modification strategies have been developed to improve the anti‐breast cancer activity of curcumin. This review focuses on the difference of modification sites and heterocyclic/non‐heterocyclic modifications to systematically summarize curcumin derivatives with better anti‐breast cancer activity.
Furostane Saponins from the Seeds of Allium ramosum and Their Lipid Accumulation Inhibitory Activity
Authors: Yen Duong Thi Hai , Tai Bui Huu , Yen Pham Hai , Nhiem Nguyen Xuan , Cuong Nguyen The , Dung Nguyen Viet , Huong Phan Thi Thanh , Cuong Pham Van , Kiem Phan Van 
Published: September 2022

AbstractThree new furostane saponins, ramofurosides A–C (1–3), and two known saponins, fistulosaponin B (4) and (25R)‐26‐O‐β‐D‐glucopyranosyl‐1β,3β,26‐trihydroxyfurosta‐5,20(22)‐diene‐1‐O‐α‐L‐rhamnopyranosyl‐(1→2)‐α‐L‐arabinopyranoside (5) were isolated from the methanol extract of Allium ramosum seeds. Their structures were identified based on spectroscopic evidence and comparison with those reported in the literature. All compounds were evaluated for reduction of lipid accumulation in HepG2 cell lines. As a result, compounds 1 and 3 showed a significant reduction in total lipid content by 27.93±3.05 and 27.54±1.68 %, respectively, at a concentration of 100 μM.
Effect of hyperglycemia on tbx5a and nppa gene expression and its correlation to structural and functional changes in developing zebrafish heart
Authors: Sankar Suruthi , Jayabalan Monisha , Venkatesh Sundararajan , Ibrahim Muhammed 
Published: September 2022

AbstractThe objective of the current study is to analyze the effects of gestational diabetes on structural and functional changes in correlation with these two essential regulators of developing hearts in vivo using zebrafish embryos. We employed fertilized zebrafish embryos exposed to a hyperglycemic condition of 25 mM glucose for 96 h postfertilization. The embryos were subjected to various structural and functional analyses in a time‐course manner. The data showed that exposure to high glucose significantly affected the embryo's size, heart length, heart rate, and looping of the heart compared to the control. Further, we observed an increased incidence of ventricular standstill and valvular regurgitation with a marked reduction of peripheral blood flow in the high glucose‐exposed group compared to the control. In addition, the histological data showed that the high‐glucose exposure markedly reduced the thickness of the wall and the number of cardiomyocytes in both atrium and ventricles. We also observed striking alterations in the pericardium like edema, increase in diameter with thinning of the wall compared to the control group. Interestingly, the expression of tbx5a and nppa was increased in the early development and found to be repressed in the later stage of development in the hyperglycemic group compared to the control. In conclusion, the developing heart is more susceptible to hyperglycemia in the womb, thereby showing various developmental defects possibly by altering the expression of crucial gene regulators such as tbx5a and nppa.
Thyroid tissue in cervical lymph nodes, not always malignant
Authors: Gijsen Anton Frans , De Bruijn Kirstin Marie Jeanne , Mastboom Walter 
Published: September 2022

AbstractThyroid tissue in cervical lymph nodes is an interesting and rare phenomenon that cannot be directly explained by embryology. Distinguishing malignant from benign thyroid tissue in cervical lymph nodes can be challenging but is essential for treatment and might have legal implications. Patients with incidentally found thyroid tissue in cervical lymph nodes during thyroid surgery were retrospectively identified. Clinical data and findings on pathology were retrospectively collected. Two patients with thyroid tissue in cervical lymph nodes were identified. Conventional pathology complemented with immunohistochemistry and molecular diagnostics showed the thyroid tissue in cervical lymph nodes to be benign. We show that benign thyroid tissue in cervical lymph nodes can be found in the absence or presence of a primary thyroid malignancy. A conservative approach is recommended if pathology shows benign thyroid tissue in cervical lymph nodes.
Predicting Interfacial Tension via the Entrainment Master Curve of a Bubble Rising through the Immiscible Liquids Interface
Authors: Edrisi Abdolaziz , Dadvar Mitra , Dabir Bahram 
Published: September 2022

AbstractLiquid‐liquid interfacial tension is an important parameter in gas‐liquid‐liquid multiphase flows. Here, bubble passage through an immiscible liquids interface was used for determining the interfacial tension. The method is based on measuring the volume of the heavy liquid entrained into the light one. Two different semi‐empirical models were developed for determining the entrainment ratio. The results are in good agreement with experiments covering a wide range of physical properties of the liquids. Each model is extended to build a master curve that can be used for predicting the interfacial tension. The models and master curves are obtained based on experimental data for gas‐phase motion through the interface between immiscible liquids, which makes the modeling results more reliable for gas‐liquid‐liquid systems.
Organic pollutants removal by Quartz crystals
Authors: Verma Sahil , Vaish Rahul 
Published: September 2022

AbstractQuartz crystals are found in nature. It is believed that these crystals are eco‐friendly and known for healing, decoration, and gem therapy. However, there is no scientific evidence of such effects, except it is a piezoelectric material. The piezocatalytic effect is viewed as having significant potential for tackling environmental challenges such as water cleaning. The piezocatalytic performance of Quartz crystals was examined and reported for a few dyes degradation. The purity of these crystals was confirmed via X‐ray diffraction, Raman spectroscopy, and X‐ray photoelectron spectroscopy analysis before experimentation. Degradation of organic dyes methylene blue, methylene violet, and Rhodamine b (RB) dyes from aqueous solutions was observed using these crystals. Naturally occurred Quartz was exposed to ultrasonic vibrations for dyes and its piezocatalytic property was studied. Furthermore, using the germination index of Cicer arientium the treated water was utilized as a growth parameter. The signature of dye degradation indicates their eco‐friendly behavior. It can be assumed that these crystals can actively participate to clean organic pollutants under the application of mechanical vibrations.
Copper‐Initiated Radical Dearomative 2,5‐Alkylarylation of Furans via Trihalomethylation/Spirocyclization Cascade
Authors: Luo Wenkun , Jiang Kai , Yin Biaolin 
Published: September 2022

Comprehensive SummaryHere is an operable and rare strategy for copper‐initiated radical dearomative 2,5‐alkylarylation of furan. By means of this strategy, we realized the dearomatization of furans to construct spirooxindoles with good yield in one‐step via trihalomethylation/spirocyclization cascade progress.
Detection of clonal mast cell disease in wasp venom allergic patients with normal tryptase
Authors: Onnes Merel C. , Alheraky Abdulrazzaq , Nawijn Martijn C. , Sluijter Tim E. , Mulder André B. , Arends Suzanne , Oude Elberink Hanneke N. G. 
Published: September 2022

AbstractBackgroundClonal mast cell disease (CMD) is an underlying aggravating condition in wasp venom allergy (WVA) which requires a different treatment strategy. CMD is increasingly recognized in patients with normal basal serum tryptase (bsT). However, methods to identify at risk patients have not yet been assessed in large cohorts of WVA patients with normal bsT.MethodsThis retrospective study evaluated the reliability of the REMA score in detecting CMD in a cohort of grade IV WVA patients with normal bsT and assessed the added value of other clinical parameters, KIT D816V mutation analysis in peripheral blood (PB) and the diagnosis of hereditary alpha tryptasemia (HAT). All patients had a conclusive bone marrow evaluation that demonstrated or excluded underlying CMD.ResultsIn total 35 CMD and 96 non‐CMD patients were included. REMA score had a sensitivity of 72% (95% CI 56%–88%) and specificity of 79% (95% CI 70%–87%) in this cohort. Loss of consciousness during systemic reaction and bsT between 6.3 and 11.4 ng/ml were additional parameters independently associated with CMD. Sensitivity of KIT in PB was relatively low, 56% (95% CI 36%–75%), but had added value as screening method in patients with a low REMA score due to 100% specificity.ConclusionThe REMA score is a relatively reliable method to detect patients at risk of CMD among WVA patients with normal bsT. KIT mutation analysis in PB could serve as additional screening method in patients with low REMA scores.
Epidemiologic outlook of therapy‐related myeloid neoplasms and selection of high‐risk patients: A Korean nationwide study
Authors: Ha Hyerim , Kim Hyo Jeong , Park Ju Hyun , Shin Aesun , Lee Kyu Na , Han Kyungdo , Lee Na Rae , Hong Junshik 
Published: September 2022

BackgroundAlthough a considerable proportion of patients with cancer receive chemotherapy (CT) or radiotherapy (RT), only a very few patients eventually develop therapy‐related myeloid neoplasms (t‐MNs).MethodsTo identify subsets of cancer patients who have substantially elevated risk of developing t‐MNs. Incidences and risks of t‐MNs after contemporary CT or RT in patients newly diagnosed major cancers during 2009–2013 were analyzed. By merging two Korean nationwide health care big data sets, patients were selected and observed on follow‐up to until t‐MN development or December 2019.ResultsAmong 250,155 patients, 555 (0.22%) were diagnosed with t‐MNs with a standard incidence ratio (SIR) of 3.40 (95% CI, 3.13–3.70). Patients had bone/joint cancers (SIR, 94.25; 95% CI, 50.71–137.80) and a remarkably high SIR for t‐MN development. Patients receiving both CT and RT had the highest SIR (4.64; 95% CI, 4.08–5.20), followed by those receiving CT only (SIR, 3.30; 95% CI, 2.89–3.70). Contrarily, RT alone did not increase t‐MN risk (SIR, 1.16; 95% CI, 0.76–1.56). More exposure to leukemogenic agents resulted in the higher t‐MNs development.ConclusionsThe increased risk of developing acute myeloid leukemia or myelodysplastic syndrome after CT and/or RT was confirmed and subsets with substantially elevated risk for developing t‐MNs were found. Such patients would be suitable for a prospective cohort for investigating t‐MN pathogenesis by time series analyses.
Interpersonal guilt and the working alliance in psychotherapy: The moderating role of childhood experience
Authors: Aafjes‐van Doorn Katie , Luo Xiaochen , Kealy David , McCollum James , Silberschatz George 
Published: September 2022

AbstractInterpersonal guilt often encompasses pathogenic beliefs that imply omnipotent responsibility for others and concerns about abandoning, humiliating or threatening others. This study sought to examine how interpersonal guilt may influence patients' and therapists' ratings of early working alliance and the potential moderating effect of perceived adverse parenting in childhood. Ninety‐five patients and their 19 therapists in an outpatient psychotherapy clinic rated their early working alliance after the first and the fifth session in treatment. We conducted separate moderation analyses for patient and therapist‐reported working alliance and controlled for psychological distress at baseline. Results suggest that perceived adverse parenting in childhood significantly moderated the effect of interpersonal guilt on the working alliance in such a way that for patients reporting very low levels of perceived adverse experiences, the interpersonal guilt beliefs had a positive effect on working alliance, whereas for those with very high levels of perceived adverse experiences, interpersonal guilt had a negative effect on working alliance. This same pattern of moderation was found for patient‐ and therapist‐reported working alliance at session 1 and therapist‐reported working alliance at session 5. Thus, although the effect of interpersonal guilt on the working alliance depends somewhat on the perspective of the working alliance rating (patient or therapist), it mainly depends on developmental experiences of the patient.
Off‐Label, but on‐Evidence? A Review of the Level of Evidence for Pediatric Pharmacotherapy
Authors: van der Zanden Tjitske M. , Smeets Nori J.L. , de Hoop‐Sommen Marika , Schwerzel Michiel F.T. , Huang Hui Jun , Barten Lieke J.C. , van der Heijden Joyce E.M. , Freriksen Jolien J.M. , Horstink Akira A.L. , Holsappel Inge H.G. , Mooij Miriam G. , de Hoog Matthijs , de Wildt Saskia N. 
Published: September 2022

Many drugs are still prescribed off‐label to the pediatric population. Although off‐label drug use not supported by high level of evidence is potentially harmful, a comprehensive overview of the quality of the evidence pertaining off‐label drug use in children is lacking. The Dutch Pediatric Formulary (DPF) provides best evidence‐based dosing guidelines for drugs used in children. For each drug‐indication‐age group combination—together compiling one record—we scored the highest available level of evidence: labeled use, systematic review or meta‐analysis, randomized controlled trial (RCT), comparative research, noncomparative research, or consensus‐based expert opinions. For records based on selected guidelines, the original sources were not reviewed. These records were scored as guideline. A total of 774 drugs were analyzed comprising a total of 6,426 records. Of all off‐label records (n = 2,718), 14% were supported by high quality evidence (4% meta‐analysis or systematic reviews, 10% RCTs of high quality), 20% by comparative research, 14% by noncomparative research, 37% by consensus‐based expert opinions, and 15% by selected guidelines. Fifty‐eight percent of all records were authorized, increasing with age from 30% in preterm neonates (n = 110) up to 64% in adolescents (n = 1,630). Many have advocated that off‐label use is only justified when supported by a high level of evidence. We show that this prerequisite would seriously limit available drug treatment for children as the underlying evidence is low across ages and drug classes. Our data identify the drugs and therapeutic areas for which evidence is clearly missing and could drive the global research agenda.
A Simple Assay for the Detection of Late‐Stage Apoptosis Features in Saccharomyces cerevisiae
Authors: Bairwa Narendra K. , Shoket Heena , Pandita Monika , Sharma Meenu 
Published: September 2022

AbstractUnicellular eukaryotic organisms such as yeast and protozoa serve as useful models for studying the impact of chemicals on cell physiology, cellular growth, and genome duplication. The yeast Saccharomyces cerevisiae has been widely used to assess apoptosis induced by chemicals due to its genetic tractability, ease of evaluation, and readily available impact assessment tools. Apoptosis in S. cerevisiae is characterized by many features, including increased cell death, loss of membrane integrity, release of caspases, chromatin condensation, and nuclear fragmentation, which are similar to the ones observed in mammalian cells. Current methods of apoptosis assessment typically require specialized equipment and reagents, which limits wide adoption. Here, we describe a rapid, inexpensive, and easy‐to‐perform assay in yeast for the analysis of late‐stage apoptotic features in cells treated with a chemical. We describe a protocol for assessing loss of cell survival and changes in the nucleus. We demonstrate the approach by using acetic acid and hydrogen peroxide as test chemicals. This assay for the study of late‐stage apoptotic features in S. cerevisiae can be performed reliably and rapidly by any laboratory with basic equipment and may be extended for studying apoptosis in similar single‐cell organisms after treatment with toxicological agents. © 2022 Wiley Periodicals LLC.Basic Protocol 1: Culture of Saccharomyces cerevisiae, treatment with acetic acid or hydrogen peroxide, and semi‐quantitative growth assayBasic Protocol 2: DAPI staining and fluorescence microscopy for the assessment of change in nucleus‐to‐cytoplasm ratio and nuclear integrity
Establishment of a Mouse Submandibular Salivary Gland Organ Culture
Authors: Sakai Manabu , Sakai Takayoshi 
Published: September 2022

AbstractThe salivary glands produce saliva and are important in maintaining oral health. Saliva keeps the mouth moist, cleanses the oral cavity, aids digestion, and has antibacterial properties. Saliva also helps in swallowing and speech. Investigating the development of the salivary glands is thus relevant in the context of both health and disease. Various cell culture methods have been used to study salivary gland development, including culturing cells in two dimensions (2D). Under physiological conditions, cells constantly interact with other cells and the extracellular matrix, which controls complex biological functions such as cell migration and apoptosis, and can modulate gene expression. Since many of these functions are not accurately represented or reproduced in 2D culture, the results of in vitro experiments using such culture methods are often not reflected in vivo. The use of 3D cultures, such as organ cultures, has helped address this issue and has emerged as a model that better reflects the in vivo physiological environment. Here, we describe a protocol for establishing submandibular salivary gland organ culture that is more concise and simpler than previous methods and includes the separation and dissection of the salivary glands. We also describe the use of environmental stress (hypoxic stimulation) and inhibitors (U0126, LY294002, and rapamycin) to elucidate signaling pathways involved in salivary gland development. This protocol can provide researchers with a simpler and more robust method of salivary gland organ culture, enabling analysis of organ‐based signaling pathways to advance developmental biology research. © 2022 Wiley Periodicals LLC.Basic Protocol 1: Submandibular salivary gland organ cultureBasic Protocol 2: Analysis of salivary gland development in the presence of hypoxia and signaling pathway inhibitorsBasic Protocol 3: Western blotting using submandibular salivary gland organ culture
Corporate social responsibility and comparative capitalism frameworks: Evidence from the United States, Poland, and Italy
Authors: Boesso Giacomo , Fryzel Barbara , Ghitti Marco 
Published: September 2022

AbstractUsing the varieties of capitalism perspective and institutional theory as frameworks, we analyze cross‐cultural differences between firms' corporate social responsibility (CSR) approaches. We investigate three countries with different institutional settings: the United States, representing a liberal market economy; Italy, representing a coordinated market economy; and Poland, an example of a dependent market economy. We examine the differences in their CSR stances, operationalized through Porter and Kramer's concept of the four CSR categories: good citizenship, mitigating harm from the value chain, transforming value chain activities, and strategic philanthropy, based on data from 269 questionnaires administered to managers attending executive seminars at the authors' universities. Focusing on stakeholder engagement activities (SEAs) across the three economies, we show that companies in all three countries engage in the four categories of CSR activities. However, using a series of tests and rankings on means and standard deviations, followed by the subsequent analysis of variance, we argue that SEAs are emphasized more in American than in Polish and Italian companies, with the latter two countries showing more subtle differences despite some institutional similarities. Our results shed light on how different capitalistic models are associated with different CSR approaches, allowing policy‐makers and practitioners to better tackle CSR objectives in different countries.
AI‐driven network softwarization scheme for efficient message exchange in IoT environment beyond 5G
Authors: Jadav Nilesh Kumar , Nair Anuja R. , Gupta Rajesh , Tanwar Sudeep , Lakys Yahya , Sharma Ravi 
Published: September 2022

SummaryInternet of things (IoT) has massively adopted the market due to the current era demanding fully intelligent and autonomous services. However, efficient network management and message exchange are challenging in a dynamic and energy‐constrained IoT environment. Hence, for efficient message passing in IoT applications, softwarization of the IoT network is essential, wherein the logical control plane is decoupled from the data plane consisting of hardware devices such as routers and switches. Using softwarization, a centralized software‐defined networking (SDN) controller is responsible for routing data packets from source to destination in a dynamic environment. However, to reduce the computational overhead of filtering malicious and nonmalicious packets, artificial intelligence (AI) classifiers prove beneficial. Moreover, such challenges have trade‐offs with network requirements such as ultralow latency, high reliability, and higher data rates. Motivated by this, we propose an AI‐enabled network softwarization scheme for efficient message exchange under the 6G network. Lastly, the performance of the proposed scheme is evaluated with different performance metrics, such as accuracy, precision, f1‐score, packet drop ratio, and latency. The empirical result revealed that the proposed scheme outperforms in terms of accuracy and controller efficiency, that is, 81.64% and 82.2%, respectively.
Cytology of malignant pleural mesothelioma: Diagnostic criteria, WHO classification updates, and immunohistochemical staining markers diagnostic value
Authors: Shaker Nada , Wu Douglas , Abid Abdul Majeed 
Published: September 2022

AbstractMalignant pleural mesothelioma (MPM) is a rare but aggressive malignancy with a poor prognosis. Because of this tumor rarity and overlapping histologic features with other malignancy types, the histopathological findings and diagnostic immunohistochemical workup are essential in establishing the final diagnosis of MPMs. We aimed to review the diagnostic criteria, WHO tumor classification updates, and immunohistochemical staining markers diagnostic value to achieve an appropriate clinical diagnosis.