bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ocular Findings of Cryptococcal Meningitis in Previously Health Adults
Authors: Okeagu Chinwenwa U. , Anjum Seher H. , Vitale Susan , Wang Jing , Singh Deven , Rosen Lindsey B. , Magone M. Teresa , Fitzgibbon Edmond J. , Williamson Peter R. 
Published: October 2022

Background:
Patients with cryptococcal meningitis (CM) often have ocular manifestations; although data are describing these findings in nonimmunosuppressed, previously healthy individuals are scarce.

Methods:
A retrospective chart review was performed for previously healthy patients with CM who underwent a complete ophthalmological examination within a 5-year period at the National Institutes of Health. Demographics, CSF parameters, findings on initial ophthalmological examination, and MRI abnormalities were analyzed.

Results:
Forty-four patients within a median of 12 weeks after CM diagnosis were included in our study; 27 patients (61%) reported abnormal vision on presentation. Seventy-one percent of patients were not shunted at the time of their initial eye examination. The most common ocular abnormalities were visual field defects in 21 (66%), decreased visual acuity in 14 (38%), and papilledema in 8 (26%) patients. Intraocular pressure was within normal range in all patients. Cranial nerve defects were identified in 5 patients and optic neuropathy in 2 patients. Patients who had hydrocephalus or did not receive a ventriculoperitoneal shunt were not noted to have worse ocular abnormalities.

Conclusions:
The most common ocular findings in our cohort of nontransplant, non-HIV cryptococcal meningitis patients were visual field defects, decreased visual acuity, and papilledema. Our results emphasize the need for a comprehensive eye examination in patients with CM who may not always report a change in vision on presentation.
Pseudomonas oryzagri sp. nov., isolated from a rice field soil
Authors: Huq Md. Amdadul , Lee Sun-Young , Ma Juncai , Rahman M. Mizanur , Rahman Md. Shahedur , Park Jong-Hyun , Akter Shahina 
Published: October 2022

A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field. The colonies were observed to be light pink-coloured, smooth, spherical and 0.6–1.0 mm in diameter when grown on nutrient agar (NA) medium for 2 days. Strain MAHUQ-58T was found to be able to grow at 15–40 °C, at pH 5.5–10.0 and with 0–1.0 % NaCl (w/v). Cell growth occurred on tryptone soya agar, Luria–Bertani agar, NA, MacConkey agar and Reasoner's 2A agar. The strain was found to be positive for both oxidase and catalase tests. The strain was positive for hydrolysis of Tween 20 and l-tyrosine. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus

Pseudomonas

and to be closely related to

Pseudomonas oryzae

WM-3T (98.9 % similarity),

Pseudomonas linyingensis

LYBRD3-7T (97.7 %),

Pseudomonas sagittaria

JCM 18195 T (97.6 %) and

Pseudomonas guangdongensis

SgZ-6T (97.2 %). The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5–89.5 % and 29.5–38.0 %, respectively. The genomic DNA G+C content was determined to be 67.0 mol%. The predominant isoprenoid quinone was ubiquinone 9. The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1
ω6c and/or C16 : 1
ω7c) and summed feature 8 (C18 : 1
ω6c and/or C18 : 1
ω7c). On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus

Pseudomonas

, for which the name Pseudomonas oryzagri sp. nov. is proposed, with MAHUQ-58T (=KACC 22005T=CGMCC 1.18518T) as the type strain.
Modeling the evolutionary history of nonclassical monocytes in mammals
Authors: Araujo Daniel J. , Fernandez Diego , Alimadadi Ahmad , Zagal-Norman Axel , Hedrick Catherine C. 
Published: October 2022

ABSTRACTThe immune system has experienced major changes in both its organization and function during the evolution of tetrapod animals. Still, the ancestry of specific cell types and the historical relationships between modern immune cells has received little attention. While all tetrapods possess mononuclear blood cells and monocytes, vasculature-patrolling nonclassical monocytes (CD16+CD14-in humans, Ly6C-in mice) have so far only been described in mammals. The question of whether nonclassical monocytes are specific to the mammalian lineage has not been answered. Cell types can be described as traits that persist via the inheritance of core regulatory complexes of transcription factors. We utilized transcriptional network analyses on a human monocyte single-cell RNA-sequencing dataset to highlight the core regulatory complex associated with nonclassical monocyte production (nCoRC). We then applied BLAST-based analyses to quantify the conservation of human nCoRC members amongst mammals and nonmammals. We determined that the average sequence similarity of nCoRC members is highest amongst mammals and that such conservation is not observed in non-mammalian tetrapods. We also discovered that this sequence similarity is specifically driven by boreoeutherian placental mammals. Finally, we found the receptor proteins upstream of nonclassical monocyte assembly are also more homologous in mammals than non-mammals. This work provides an evolutionary model in which the capacity for nonclassical monocyte production is unique to the mammalian lineage.
Non-triplet genetic code inEuplotesciliates is a result of neutral evolution
Authors: Gaydukova Sofya , Moldovan Mikhail , Vallesi Adriana , Heaphy Stephen M. , Atkins John F , Gelfand Mikhail S. , Baranov Pavel V. 
Published: October 2022

ABSTRACTAlthough several variants of the standard genetic code are known, its triplet character is universal with an exception in ciliatesEuplotes, where stop codons at internal mRNA positions specify ribosomal frameshifting. How didEuplotesspp. evolved and maintained such an unusual genetic code remains a mystery. To investigate these questions, we explored the evolution of frameshifting occurrence in Euplotes genes. We sequenced and analyzed several transcriptomes from differentEuplotesspp to characterize the gain-and-loss dynamics of frameshift sites. Surprisingly, we found a sharp asymmetry between frameshift gain and frameshift loss events with the former exceeding the latter by about 10 folds. Further analysis of mutation rates in protein-coding and non-coding regions revealed that this asymmetry is expected based on single nucleotide mutation rates and does not require positive selection for frameshifting. We found that the number of frameshift sites inEuplotesspp is increasing and is far from the steady state. The steady equilibrium state is expected in about 0.1 to 1 billion years leading to about a 10 fold increase in the number of frameshift sites in Euplotes genes.
Deep learning based decoding of local field potential events
Authors: Schilling Achim , Gerum Richard , Boehm Claudia , Rasheed Jwan , Metzner Claus , Maier Andreas , Reindl Caroline , Hamer Hajo , Krauss Patrick 
Published: October 2022

AbstractHow is information processed in the cerebral cortex? To answer this question a lot of effort has been undertaken to create novel and to further develop existing neuroimaging techniques. Thus, a high spatial resolution of fMRI devices was the key to exactly localize cognitive processes. Furthermore, an increase in time-resolution and number of recording channels of electro-physiological setups has opened the door to investigate the exact timing of neural activity. However, in most cases the recorded signal is averaged over many (stimulus) repetitions, which erases the fine-structure of the neural signal. Here, we show that an unsupervised machine learning approach can be used to extract meaningful information from electro-physiological recordings on a single-trial base. We use an auto-encoder network to reduce the dimensions of single local field potential (LFP) events to create interpretable clusters of different neural activity patterns. Strikingly, certain LFP shapes correspond to latency differences in different recording channels. Hence, LFP shapes can be used to determine the direction of information flux in the cerebral cortex. Furthermore, after clustering, we decoded the cluster centroids to reverse-engineer the underlying prototypical LFP event shapes. To evaluate our approach, we applied it to both neural extra-cellular recordings in rodents, and intra-cranial EEG recordings in humans. Finally, we find that single channel LFP event shapes during spontaneous activity sample from the realm of possible stimulus evoked event shapes. A finding which so far has only been demonstrated for multi-channel population coding.
Analyses of single extracellular vesicles from non-small lung cancer cells to reveal effects by Epidermal growth factor inhibitor treatments
Authors: Stridfeldt Fredrik , Cavallaro Sara , Hååg Petra , Lewensohn Rolf , Linnros Jan , Viktorsson Kristina , Dev Apurba 
Published: October 2022

AbstractPrecision cancer medicine have changed the treatment landscape of non-small cell lung cancer (NSCLC) as illustrated by tyrosine kinase inhibitors (TKIs) towards mutated Epidermal growth factor receptor (EGFR). Yet, responses to such TKIs e.g., erlotinib and osimertinib among patients are heterogenous and there is a need for non-invasive blood-based analytics to follow treatment response and reveal resistance to improve patient’s treatment outcome. Recently, extracellular vesicles (EVs) have been identified as an important source of tumor biomarkers promising to revolutionize liquid biopsy-based diagnosis of cancer. However, high heterogeneity has been a major bottleneck. The pathological signature is often hidden in the differential expression of membrane proteins in a subset of EVs which are difficult to identify with bulk techniques. Using a fluorescence-based approach, we for the first time demonstrate that the single-EV technique can be used to monitor the treatment response of targeted cancer therapies such as TKIs towards EGFR. To test the hypothesis, we analyzed the membrane proteins of native EVs extracted from EGFR-mutant NSCLC cell line, both prior and post treatment with EGFR-TKIs erlotinib or osimertinib. The selected cell line being refractory to erlotinib and responsive to osimertinib makes it a suitable model system. The expression level of five surface proteins; two common tetraspanins (CD9, CD81) and three markers of specific interest in lung cancer (EGFR, PD-L1, HER2) were studied. The data suggest that in contrast to erlotinib, the osimertinib treatment increases the population of PD-L1, EGFR and HER2 positive EVs while the expression level per EV decreases for all the three markers. The PD-L1 and HER2 expressing EV population seems to increase by several fold because of osimertinib treatment. The observations agree with the previous reports performed on cellular level indicating the biomarker potential of EVs for liquid-biopsy based monitoring of targeted cancer treatments.HighlightsMembrane protein analyses of single EVs may reveal distinct differences when lung cancer cells are refractory vs responsive under different EGFR-TKI treatments.Comparison of 1stgeneration erlotinib and 3rdgeneration osimertinib shows clear signature on the expression of PD-L1, EGFR, HER2 on single EVsColocalization showed a change in common marker combinations before after treatment.PD-L1 expression per vesicle decreases while the number of PD-L1 positive EVs increases as a result of osimertinib treatment, indicating that such signature may not be detectable under bulk analysis
Canonical and Non-Canonical Psychedelic Drugs Induce Common Network Changes in Human Cortex
Authors: Dai Rui , Larkin Tony E. , Huang Zirui , Tarnal Vijay , Picton Paul , Vlisides Phillip E. , Janke Ellen , McKinney Amy , Hudetz Anthony G. , Harris Richard E. , Mashour George A. 
Published: October 2022

AbstractThe neurobiology of the psychedelic experience is not fully elucidated. Identifying common brain network changes induced by both canonical (i.e., acting at the 5-HT2 receptor) and non-canonical psychedelics would provide mechanistic insight into state-specific characteristics. We analyzed whole-brain functional connectivity based on resting-state fMRI data in humans, acquired before and during the administration of nitrous oxide, ketamine, and lysergic acid diethylamide. We report that, despite distinct molecular mechanisms and modes of delivery, all three psychedelics reduced within-network functional connectivity and enhanced between-network functional connectivity. More specifically, all drugs tested increased connectivity between right temporoparietal junction and bilateral intraparietal sulcus as well as between precuneus and left intraparietal sulcus. These regions fall within the posterior cortical “hot zone,” posited to mediate the content of consciousness. Thus, both canonical and non-canonical psychedelics modulate networks within an area of known relevance for conscious experience, identifying a biologically plausible candidate for their subjective effects.
Baculovirus Surface Display of Hemagglutinin and Neuraminidase for Monoclonal Antibody Production
Authors: Lo Huei-Ru , Wu Chun-Pei , Jan Jia-Tsrong , Chao Yu-Chan , Tsai Chih-Hsuan 
Published: October 2022

AbstractThe H7N9 influenza virus that emerged in 2013 is a dangerous infectious disease with a high mortality rate of up to 40%. Developing effective monoclonal antibodies (mAbs) to detect and treat the infection of this virus is therefore critical. In this study, we expressed hemagglutinin (HA) and neuraminidase (NA) of H7N9 (A/Anhui/1/2013) on the surface of baculovirus (i.e., HA7-Bac and NA9-Bac). Our results showed that both HA or NA proteins displayed on HA7-Bac or NA9-Bac could well maintain their native biological function. Mice antisera derived from the injections of either HA7-Bac- or NA9-Bac exhibited high inhibitory activity in the hemagglutination and neuraminidase assay of H7N9 virus. mAbs generated by immunization with HA7-Bac exhibited high neutralizing activity against H7N9 virus infectivity in cell assays, whereas mAbs generated by immunization with NA9-Bac inhibited neuraminidase activity. These results proved that baculovirus display of HA and NA from H7N9 could be convenient agents to generate neutralizing mAbs against virus infection.
Reclassification of probioticLactobacillus acidophilusNCIMB 30184 asLactobacillus helveticusandLactobacillus caseiNCIMB 30185 asLacticaseibacillus paracasei
Authors: Rodenes A. , Casinos B. , Alvarez B. , Martinez-Blanch J.F. , Vijayakumar V. , Superti G. , Day R. , Chenoll E. 
Published: October 2022

ABSTRACTThe strains NCIMB 30184 and NCIMB 30185, originally designated asLactobacillus acidophilus and Lactobacillus caseirespectively, have been studied in published pre-clinical and clinical studies. Their nucleotide sequences of the 16S rRNA were compared with public databases and were preliminarily found to have the highest similarity withLactobacillus helveticusandLacticaseibacillus paracaseispecies respectively. Subsequently, total 16S and 23S rRNA genes were compared with their most similar taxa, and the preliminary identification asLactobacillus helveticusandLacticaseibacillus paracaseiwere confirmed.
Temporal and regulatory dynamics of the inner ear transcriptome during development in mice
Authors: Cao Rui , Takechi Masaki , Wang Xiuwan , Furutera Toshiko , Nojiri Taro , Koyabu Daisuke , Li Jun 
Published: October 2022

AbstractThe inner ear controls hearing and balance, while the temporal molecular signatures and transcriptional regulatory dynamics underlying its development are still unclear. In this study, we investigated time-series transcriptome in the mouse inner ear from embryonic day 11.5 (E11.5) to postnatal day 7 (P7) using bulk RNA-Seq. A total of 10,822 differentially expressed genes were identified between pairwise stages. We identified nine significant temporal expression profiles using time-series expression analysis. The constantly down-regulated profiles throughout the development are related to DNA activity and neurosensory development, while the constantly up-regulated profiles are related to collagen and extracellular matrix. Further co-expression network analysis revealed that several hub genes, such as pnoc1, cd9, and krt27, are related to the neurosensory development, cell adhesion, and keratinization. We uncovered three important transcription regulatory paths during mice inner ear development. Transcription factors related to Hippo/TGFβ signaling induced decreased expressions of genes relate to the neurosensory and inner ear development, while a series of INF genes activated the expressions of genes in immunoregulation. In addition to deepening our understanding of the temporal and regulatory mechanisms of inner ear development, our transcriptomic data could fuel future multi-species comparative studies and elucidate the evolutionary trajectory of auditory development.
Customers create customers!–Assessing the role of perceived personalization, online advertising engagement and online users' modes in generating positive e-WOM
Authors: Noor Uzma , Mansoor Mahnaz , Shamim Amjad 
Published: October 2022

PurposeThis study examined the relationship between perceived personalization and positive electronic word of mouth, as well as the mediating impact of online advertising engagement and the moderating effect of online users' modes on that relationship. The theory of presence serves as the foundation for the relationships among variables.Design/methodology/approachA quasi-experimental research design was used to carry out the investigation. The analysis was performed on 865 valid responses from the treatment and control groups.FindingsResults showed no mediation for the control group and partial mediation for the treatment group. The treatment group's moderated mediation relationships were found as significant, but the control group's relationships were insignificant. The findings also supported the hypothesis that there is a significant association between perceived personalization and online advertising engagement for playful online users and a weak relationship for serious online users. On the mediation link of online advertising engagement between perceived personalization and positive electronic word of mouth, the conditional indirect influence of “online users' modes” has been specifically studied.Originality/valueThis study is the first to examine online advertising through the lens of the theory of presence and offers a moderated-mediation model of Online Users' Modes and Online Advertising Engagement, which is a valuable addition to the marketing body of knowledge.
Impact of GST on motor and pump exports in India
Authors: Negi Raghuveer , Arumugam Muthusamy , Nomani Abuzar , Geeta Shetty Deepa Thangam 
Published: October 2022

PurposeThe impact of Goods and Service Tax (GST) on the motor and pump exports in the Coimbatore region is measured in this study using various parameters and scales. The data collected from exporters were used to identify the pros and cons of GST, stating their opinions on variables considered by the researchers through extensive literature on GST and exports.Design/methodology/approachThe data were collected from 220 motors and pump exporters through a field survey from the month of January to September 2021. The impacts have been measured using principal component analysis (PCA) and confirmatory factor analysis (CFA). The factor analyses and CFA will derive the positive and negative impact of GST determined through critical empirical evidence in this study. Also, the problems faced by the exporters allied to GST, which the authors could not include in the questionnaire due to certain reasons, are concisely apportioned and discussed.FindingsThe study depicts the major advantages of GST, such as harmonized system, long-run performance, reduction in logistics cost, check-post operation, bonds and ease of doing business. Also, it highlights the disadvantages of GST, such as biases in the indirect tax system, the reimbursement of duty drawback being late or pending and document filing was still a tedious job under the GST regime.Originality/valueThe unavailability of considerable literature on the impact of GST on Indian exports signifies the novelty of this research. So far, this is the first empirical attempt to measure the impact of GST on exports which is a unique and original attempt to highlight the problem that lies under the GST regime and the necessary reforms the tax structure needs in the context of Indian exports.
The pharaoh rises: Z-generation startup PIK
Authors: El Bedawy Randa , Elsayed Mayar Farrag 
Published: October 2022

Learning outcomesThe case can be used to discuss leadership issues in young ventures. It also allows for a discussion of effective sales and marketing functions for a new product. The case also addresses the different challenges facing certain business models in the market, and finally the importance of having a supporting team.Case overview/synopsisThe case traces the development of an Egyptian startup software venture and the challenges faced regarding the entrepreneurship ecosystem. The startup’s main dilemma is whether to continue in its existing market or to extend elsewhere. Launching the main services of the startup is another challenge, as the market needs to be educated to see its value. Despite the success story, as a young Egyptian entrepreneur, the founder is expected to face various challenges to excel in the Egyptian market, such as financing, marketing, teamwork and self-development of the entrepreneur himself.Complexity academic levelThe case can be used at all levels, from undergraduate and MBA classes to executive seminars, because the issues it addresses are of broad interest.Supplementary materialsTeaching notes are available for educators only.Subject codeCSS 3: Entrepreneurship.
Identifying the relationship between abusive supervision and organizational members’ deviant behavior, in-role behavior and work–life balance
Authors: Cho Taejun , Park Yongho , Jang Jaeyeon 
Published: October 2022

Purpose
The purpose of this study is to investigate the relationships among abusive supervision, in-role behavior, career commitment and work–life balance.

Design/methodology/approach
The data were collected from 310 South Korean company employees using the survey method. To investigate the research hypotheses, structural equation modeling analysis was conducted.

Findings
This study found negative effects of abusive supervision on in-role behavior, career commitment and work–life balance. Career commitment and work–life balance has the positive influences on in-role behavior. These results support the research hypotheses.

Research limitations/implications
Although this study empirically confirmed the negative effects of abusive supervision on employees’ attitudes toward their careers, lives and working behavior, the influence of cultural aspects was not considered. This study found mediating effects of work–life balance and career commitment.

Practical implications
This study points out that one leader with abusive supervision can negate all organizational efforts aimed at employees’ well-being because the influence of leaders on employees’ careers, lives and working behavior is very critical.

Originality/value
This paper provides a comprehensive understanding of the relationships between abusive supervision and other related variables from a human resource development perspective.