bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex differences in the impact of electronic nicotine vapor on corticotropin-releasing factor receptor 1 neurons in the mouse ventral tegmental area
Authors: Zhu ManHua , Rogers Neil G , Jahad Jasmine V , Herman Melissa A 
Published: August 2022

AbstractNicotine engages dopamine neurons in the ventral tegmental area (VTA) to encode reward and drive the development of nicotine addiction, however how nicotine selectively alters other VTA populations remains to be determined. Here, we used male and female CRF1-GFP mice and nicotine vapor exposure to examine the effects of nicotine in VTA corticotropin-releasing factor receptor 1 (CRF1) neurons. We use immunohistochemistry and electrophysiology to examine neuronal activity, excitability, and inhibitory signaling. We found that VTA CRF1 neurons are mainly dopaminergic and project to the nucleus accumbens (VTA-NAcCRF1 neurons).VTA-NAcCRF1 neurons show greater phasic inhibition in naïve females and greater focal nicotine-induced increases in firing in naïve males. Following acute nicotine vapor exposure, phasic inhibition was not altered, but focal nicotine-induced tonic inhibition was enhanced in females and diminished in males. Acute nicotine vapor exposure did not affect firing inVTA-NAcCRF1 neurons, but females showed lower baseline firing and higher focal nicotine-induced firing. Activity (cFos) was increased in the CRF1 dopaminergic VTA population in both sexes, but with greater increases in females. Following chronic nicotine vapor exposure, both sexes displayed reduced basal phasic inhibition and the sex difference in tonic inhibition following acute vapor exposure was no longer observed. Additionally, activity of the CRF1 dopaminergic VTA population was no longer elevated in either sex. These findings reveal sex- and exposure-dependent changes in mesolimbic VTA-NAc CRF1 neuronal activity, inhibitory signaling, and nicotine sensitivity following nicotine vapor exposure. These changes potentially contribute to nicotine-dependent behaviors and the intersection between stress, anxiety, and addiction.
Mapping Hsp104 structure and substrate interactions using crosslinking mass spectrometry
Authors: Westphal Kinga , Joachimiak Lukasz A. 
Published: August 2022

AbstractMolecular machines from the AAA+ family play important roles in protein folding, disaggregation and DNA processing. Recent cryo-EM structures of AAA+ molecular machines have uncovered nuanced changes in conformation that underlie their specialized functions. Furthermore, complexes between these machines and substrates begin to explain their mechanism of activity. Here we explore how crosslinking mass spectrometry (XL-MS) can be used to interpret changes in conformation induced by ATP and how substrates are associated. We applied a panel of crosslinking reagents to produce high-resolution crosslinking maps and interpret our data on previously determined X-ray and cryo-EM structures of Hsp104 from a thermophilic yeast, Calcarisporiella thermophila. We developed an analysis pipeline to differentiate between intra-subunit and inter-subunit contacts within the hexameric homo-oligomer. We identify crosslinks that break the asymmetry that are only present when ATP is bound and are absent in an ATP-binding deficient mutant. Finally, we identify contacts between Hsp104 and a model substrate to identify contacts on the central channel of Hsp104 across the length of the substrate indicating that we have trapped interactions consistent with translocation of the substrate. Our simple and robust XL-MS-based experiments and methods help interpret how these molecular machines change conformation and bind to substrates even in the context of homo-oligomeric assemblies.
Disrupted chromatin architecture in olfactory sensory neurons: A missing link from COVID-19 infection to anosmia
Authors: Tan Zhen Wah , Toong Ping Jing , Guarnera Enrico , Berezovsky Igor N. 
Published: August 2022

AbstractWe tackle here genomic mechanisms of a rapid onset and recovery from anosmia - a useful diagnostic indicator for early-stage COVID-19 infection. On the basis of earlier observed specifics of olfactory receptors (ORs) regulation in the mice chromatin structures, we hypothesized that the disruption of OR function can be caused by chromatin reorganization taking place upon SARS-CoV-2 infection. We reconstructed the chromatin ensembles of ORs obtained from COVID-19 patients and control samples using our original computational framework for the whole-genome chromatin ensemble 3D reconstruction. We have also developed here a new procedure for the analysis of fine structural hierarchy in local, megabase scale, parts of chromosomes containing the OR genes and corresponding epigenetic factors. We observed structural modifications in COVID-19 patients on different levels of chromatin organization, from alteration of the whole genome structure and chromosomal intermingling to reorganization of contacts between the chromatin loops at the level of topologically associating domains. While complementary data on known regulatory elements point to pathology-associated changes within the overall picture of chromatin alterations, further investigation using additional epigenetic factors mapped on 3D reconstructions with improved resolution will be required for better understanding of anosmia caused by SARS-CoV-2 infection.
Isolation and Transfection of Rice Egg Cells and Zygotes for Cellular Localization
Authors: Xu Hengping , Bartley Laura , Libault Marc , Sundaresan Venkatesan , Fu Hong , Russell Scott 
Published: August 2022

AbstractDue to the difficulty in accessing to gametes and zygotes in flowering plants, which are controlled in single cells deeply embedded in multiple tissues, little is known about how the initiation of plant embryogenesis may reflect or contrast from such systems in other eukaryotes. In this study we has developed an approach of isolation and transfection of rice egg cells and zygotes for cellular localization of rice cell cycle factors (KRP5, KRP4 and FB3), which opened a pathway to monitor protein expression in rice egg cells and zygotes at different developmental stages. The advantageous feature of isolated rice cells may serve as an ideal system for studying the molecular mechanism underlying the rice zygotic division to initiate seed formation.
Gigaxonin is required for intermediate filament transport
Authors: Renganathan Bhuvanasundar , Zewe James P , Cheng Yuan , Kittisopikul Mark , Opal Puneet , Ridge Karen M , Gelfand Vladimir I. 
Published: August 2022

AbstractGigaxonin is an adaptor protein for E3 ubiquitin ligase substrates. It is necessary for ubiquitination and degradation of intermediate filament (IF) proteins. Giant axonal neuropathy is a pathological condition caused by mutations in the GAN gene that encodes gigaxonin. This condition is characterized by abnormal accumulation of IFs in both neuronal and non-neuronal cells; however, it is unclear what causes IF aggregation. In this work, we studied the dynamics of IFs using their subunits tagged with a photoconvertible protein mEOS 3.2. We have demonstrated that the loss of gigaxonin dramatically inhibited transport of IFs along microtubules by the microtubule motor kinesin-1. This inhibition was specific for IFs, as other kinesin-1 cargoes, with the exception of mitochondria, were transported normally. Another effect of gigaxonin loss was a more than 20-fold increase in the amount of soluble vimentin oligomers in the cytoplasm of gigaxonin knock-out cells. We speculate that these oligomers saturate a yet unidentified adapter that is required for kinesin-1 binding to IFs, which might inhibit IF transport along microtubules causing their abnormal accumulation.
Self-regulation of the nuclear pore complex enables clogging-free crowded transport
Authors: Zheng Tiantian , Zilman Anton 
Published: August 2022

AbstractNuclear pore complexes (NPCs) are the main conduits for macromolecular transport into and out of the nucleus of eukaryotic cells. The central component of the NPC transport mechanism is an assembly of intrinsically disordered proteins (IDPs) that fills the NPC channel. The channel interior is further crowded by large numbers of simultaneously translocating cargo-carrying and free transport proteins. How the NPC can efficiently, rapidly and selectively transport varied cargoes in such crowded conditions remains ill understood. Past experimental results suggest that the NPC is surprisingly resistant to clogging and that transport may even become faster and more efficient as the concentration of transport protein increases. To understand the mechanisms behind these puzzling observations, we construct a computational model of the NPC comprising only a minimal set of commonly-accepted consensus features. This model qualitatively reproduces the previous experimental results and identifies self-regulating mechanisms that relieve crowding. We show that some of the crowding-alleviating mechanisms – such as preventing saturation of the bulk flux – are “robust” and rely on very general properties of crowded dynamics in confined channels, pertaining to a broad class of selective transport nanopores. By contrast, the counter-intuitive ability of the NPC to leverage crowding to achieve more efficient single molecule translocation is “fine-tuned” and relies on the particular spatial architecture of the IDP assembly in the NPC channel.
A living conductive marine biofilm engineered to sense and respond to small molecules
Authors: Bird Lina J. , Leary Dasha , Hervey Judson , Compton Jaimee , Phillips Daniel , Tender Leonard M. , Voigt Christopher A. , Glaven Sarah M. 
Published: August 2022

AbstractEngineered electroactive bacteria have potential applications ranging from sensing to biosynthesis. In order to advance the use of engineered electroactive bacteria, it is important to demonstrate functional expression of electron transfer modules in chassis adapted to operationally relevant conditions, such as non-freshwater environments. Here, we use the Shewanella oneidensis electron transfer pathway to induce current production in a marine bacterium, Marinobacter atlanticus, during biofilm growth in artificial seawater. Genetically encoded sensors optimized for use in E. coli were used to control protein expression in planktonic and biofilm attached cells. Significant current production required addition of menaquinone, which M. atlanticus does not produce, for electron transfer from the inner membrane to the expressed electron transfer pathway. Current through the S. oneidensis pathway in M. atlanticus was observed when inducing molecules were present during biofilm formation. Electron transfer was also reversible, indicating electron transfer into M. atlanticus could be controlled. These results show that an operationally relevant marine bacterium can be genetically engineered for environmental sensing and response using an electrical signal.
Temporal and spatial staging of lung alveolar regeneration is determined by the grainyhead transcription factor Tfcp2l1
Authors: Cardenas-Diaz Fabian L. , Liberti Derek C. , Leach John P. , Babu Apoorva , Barasch Jonathan , Shen Tian , Diaz-Miranda Maria A. , Zhou Su , Ying Yun , Morley Michael P. , Morrisey Edward E. 
Published: August 2022

ABSTRACTAlveolar epithelial type 2 (AT2) cells harbor the facultative progenitor capacity in the lung alveolus to drive regeneration after lung injury. Using single cell transcriptomics, software-guided segmentation of tissue damage, and in vivo lineage tracing, we have identified the grainyhead transcription factor Tfcp2l1 as a key regulator of this regenerative process. Tfcp2l1 expression is initiated late in lung development and restricted to the AT2 cell population in the postnatal lung. Loss of Tfcp2l1 in adult AT2 cells decreased self-renewal and enhanced AT2-AT1 differentiation during active tissue regeneration. Conversely, Tfcp2l1 blunts the proliferative response to inflammatory signaling during the early acute phase after injury. This ability of Tfcp2l1 to temporally regulate the balance of AT2 self-renewal and differentiation is spatially restricted to zones undergoing active alveolar regeneration. Single-cell transcriptomics and lineage tracing reveal that Tfcp2l1 regulates cell fate dynamics by balancing the traffic across the AT2-AT1 differentiation axis and restricting the inflammatory program in AT2 cells. Organoid modeling shows that these cell fate dynamics are controlled by Tfcp2l1 regulation of IL-1 receptor expression and activity in AT2 cells. Together, these studies reveal the critical importance of properly staging lung alveolar regeneration and the integral role of Tfcp2l1 plays in balancing epithelial cell self-renewal and differentiation in this process.
Interests, motivations and gastronomic experiences in the world heritage site destination of Granada (Spain): satisfaction analysis
Authors: Valverde-Roda José , Medina Viruel Miguel Jesús , Castaño Prieto Lucía , Solano Sánchez Miguel Ángel 
Published: August 2022

PurposeGastronomy can be a key destination choice factor. As tourists, people will be able to learn more about the culture of the place through its culinary assets. This paper aims to analyse the interest and the gastronomic motivations of tourists to the city of Granada (Spain), where two important UNESCO World Heritage Sites (WHS) are included.Design/methodology/approachTo achieve the aim of this study, fieldwork was carried out on a representative sample of tourists in Granada (Spain). Specifically, a total of 1,612 valid surveys were filled out in culinary establishments and historical sites. In these surveys, the opinion of tourists regarding gastronomy and their motivations when travelling was assessed.FindingsThe results of this research allow to make a segmentation of tourists into three groups according to their position and their interest in gastronomy based on their destination choice, distinguishing among survivors, enjoyers and experiencers’ tourists. Additionally, it is confirmed that gastronomy is shaped as a motivation that influences the level of tourist satisfaction, performing as a differentiating element that can help increase the competitiveness of the destination.Originality/valueThis research contributes to the scarce academic literature on tourism experiences in a city with WHS recognitions. This study confirms the existence of a relationship between gastronomic motivations and the level of satisfaction achieved by tourists who visit the city of Granada, where no similar studies were found. In addition, this work confirms the connection between gastronomy and culture.
Reducing early-stage Cleantech funding gaps: an exploration of the role of Environmental Performance Indicators
Authors: Harrer Theresia , Owen Robyn 
Published: August 2022

PurposeThe purpose of this paper is to explore why, despite the development of a hybrid investing logic, funding problems are so persistent for early-stage Cleantech ventures (“Cleantechs”). An institutional logics lens is adopted to analyze how key actors' perceptions and communications of the Cleantech value proposition shape information asymmetries (IAs).Design/methodology/approachA mixed methods approach draws on 82 Cleantech pitch decks and 31 investment guidance documents, and insights from interviews with 42 key informants and nine Cleantech CEOs and their investors.FindingsIAs persist, first of all, because key investor and entrepreneurial actors combine different goals in the hybrid Cleantech value proposition. Interestingly, the analysis of Environmental Performance Indicators (EPIs) as a critical communication tool reveals a further mismatch in how actors actually combine logics. The authors ultimately identify three emergent actor roles – traditional laggard, developer and boundary spanner – that present a framework of how the three most influential actor groups develop EPIs and via that a hybrid Cleantech financing logic to overcome IAs.Originality/valueThe paper enhances the entrepreneurial finance literature primarily by showing that in contexts of hybrid investing a more nuanced understanding of institutional logics in terms of ends and means is critical to overcome IAs. While prior works highlight goal incompatibilities, the findings here suggest that the (in-)compatibility of goals as well as EPI choices of the same actors is likely to be the key explanandum for the stickiness of IAs and the funding gap. The novel emerging role framework offers additional theoretical, policy and practical advances for hybrid logic development.
Continuous participation intention in on-demand logistics: interactive effects of order assignment and delivery-related information disclosure strategies
Authors: Zhao Quanwu , Yuan Jiamin , Liu Yuqing , Yang Jiaqin 
Published: August 2022

PurposeCouriers are in an unequal relationship with on-demand logistic platforms with regards to order assignment and delivery-related information acquisition, which leads to high courier turnover rates. Based on social cognitive theory and justice theory, this research investigates the impact of order assignment and delivery-related information disclosure strategy on couriers' perceived justice and continuous participation intention and presents managerial suggestions to on-demand logistic platforms to lower the courier turnover rate.Design/methodology/approachTaking Chinese couriers as experimental subjects, this study conducts experiments by constructing an order receiving scene of order assignment strategy (performance-priority vs distance-priority) and delivery-related information disclosure strategy (detailed-information vs brief-information) and analyzes the results of 452 valid respondents.FindingsThe results indicate that the interaction between order assignment and delivery-related information disclosure strategy in on-demand logistics significantly affects couriers’ continuous participation intention, specifically under performance-priority order assignment and detailed-information (vs brief-information) disclosure strategy. Informational justice and distribution justice play mediating roles, and work experience and proactive personality moderate the relationship interactions.Practical/Social implicationsThe research helps us to understand the order-receiving justice demand and delivery-related information demand of couriers in on-demand logistics platforms and sheds light on cutting down turnover rates through different strategies designs and justice environment construction.Originality/valueThis research integrates social cognitive theory into on-demand logistics and combines with justice theory to fill platform strategies, couriers’ justice perception and characteristics, as well as behavior into “triadic reciprocal causation.” Meanwhile, it investigates different impacts and interactive relationships of order assignment and delivery-related information disclosure strategy, expands strategies from the impact of operational efficiency to the impact of couriers’ participation and extends the literature of justice perception and individual characteristics in on-demand logistics.
The mediating effect of knowledge management on talent management and firm performance in small and medium enterprise in Uganda
Authors: Bagorogoza Janet Kyogabiirwe , Nakasule Idah 
Published: August 2022

PurposeThis study examines the mediating effect of an effective knowledge management (KM) in the relationship between talent management (TM) and organizational performance. This study was operationalized among the small and medium enterprises (SMEs).Design/methodology/approachA cross-sectional survey design was used to collect data from a sample of 260 SMEs business. The data collected was analyzed using correlations and hierarchical regression to test the mediating effect of KM on the relationship between TM and firm performance.FindingsThe findings show that KM mediates the relationship between TM and firm performance. They also suggest that TM contribute to enhancing performance through improvements in KM.Research limitationsThe sample used in the research is not representative of all the SMEs operating in Uganda. This limitation presents the challenge of generalising and we therefore suggest caution when interpreting the results. We note the challenge of a skewed sample. For instance, most of the respondents to our study were confined to tailoring and design and metal and fabrication sectors. The skewed sample is a notable limitation of the research. The study was limited to Uganda only hence it did not capture information relating to other geographical settings, which may limit the effectiveness of the findings therein.Practical implicationsBased on the findings of the study, the managers of the SME can use the study results to develop strategies and interventions that can enable their firms to improve on their performance even in the harsh economic environment through adopting positive practices such as KM.Originality/valueThis paper contributes by filling a gap in HR management and SMEs literature, in which empirical studies on the relationship between TM and firm performance have been limited until now. This may create better research opportunities for cross-disciplinary papers that should be done by human resource, small business management and KM scholars.
Social networking sites adoption among entrepreneurial librarians for globalizing startup business operations
Authors: Gupta Varun , Rubalcaba Luis , Gupta Chetna , Pereira Leandro 
Published: August 2022

PurposeThe purpose of this study is to identify the factors leading to adoption of social networking sites (SNS) by librarians of entrepreneurial libraries to provide support to business community in their market research. Identification of these adoption factors will help to design SNS in the way that led to their seamless diffusion in university libraries, making them capable of providing reliable and useful market knowledge to the business community.Design/methodology/approachTo meet the research objectives, this study is based on the technology adoption model (TAM). The survey was conducted with 50 librarians of various universities across Europe, Asia, America, Africa and Australia, sampled through non probabilistic sampling techniques-purposive and snowball sampling. The collected data are analyzed through partial least squares structural equation modeling (PLS-SEM) to estimate the formulated structural equation model using SmartPLS 3.2.9 software version.FindingsThe results indicate that university librarians have high perception about usefulness and ease of using SNS for meeting their business objectives. The perceived usefulness (PU) is solely based on the prolonged adoption of SNS technology in personal lives of the librarians. The perceived ease of use (PEU) is triggered by the increasing quality of evolving SNS technology, especially user friendliness. The prolonged use of technology, increasing market entry of brands and improving quality of SNS will overcome librarian resistance to use SNS for market research related support to business community. Evolving technologies are more likely to be adopted seamlessly in entrepreneurial libraries.Research limitations/implicationsThis study has implications for librarians, entrepreneurs and technology providers. Librarians should have flexible technology adoption policies in place to keep technology adoption synchronized with changing market conditions. Entrepreneurs should share clear and accurate business needs as well as practicing ethical and lawful business practices. They should also help libraries to gain new skills to make future adoption easier. Technology providers should focus on improving the quality of their technology by increasing their functional utilities, performance and reliability as well as making them more user-friendly.Originality/valueThis study investigates technology adoption of SNS in libraries from the perspective of providing market research related services to entrepreneurs rather than just digitalizing library services for student users.