bluuebunny/crossref_metadata_2025_split huggingface dataset:
Septins regulate virulence in Verticillium dahliae and differentially contribute to microsclerotial formation and stress responses
Authors: Wang Haifeng , Tang Chen , Deng Chenglin , Li Wenwen , Klosterman Steven J. , Wang Yonglin 
Published: November 2022

AbstractSeptin proteins play a role in the formation of hyphal septa as well as in the division of nuclei, cytoskeletal organization, and cell morphogenesis in filamentous fungi. Herein, we investigated the functions of four septin-coding genes (VdSep3, VdSep4, VdSep5, and VdSep6) in the plant pathogenic fungus Verticillium dahliae. Microsclerotial formation was positively regulated by VdSep4 and VdSep6, whereas VdSep3 and VdSep5 had no effect on microsclerotial development but their deletion slightly reduced melanin production. Deletion of VdSep4 or VdSep5, but not VdSep3, resulted in hypersensitivity to high-temperature stress. Deletion of VdSep4 led to increased benomyl sensitivity whereas deletion of VdSep3 showed increased benomyl resistance. The previously reported roles of VdSep3 and VdSep5 in virulence were confirmed. Disruption of each of the four septin-coding genes led to reduction in penetration peg formation and hyphal expansion into plant cells. Loss of VdSep3 or VdSep4 increased sensitivity to reactive oxygen species (ROS) and reactive nitrogen species (RNS) stress, whereas VdSep6 played a role in RNS stress response but not in ROS stress response. In addition, all the septin gene deletion mutants showed an abnormality in chitin distribution but varied in their responses to several stresses examined. Taken together, our results indicate that members of the septin family in V. dahliae play different roles in regulating microsclerotial development, melanin synthesis, and stress responses, while they are all required for full virulence of the fungus.
Alcohol use and its association with suicide attempt, suicidal thoughts and non-suicidal self-harm in two successive, nationally representative English household samples
Authors: Ledden Sarah , Moran Paul , Osborn David , Pitman Alexandra 
Published: November 2022

Background
Alcohol use is a risk factor for suicidal behaviour, yet the nature of the relationship is unclear. Most research on the topic is conducted in clinical populations, with few studies exploring this association across the general population.

Aims
We investigated the association between specific domains of alcohol use and suicide attempt, suicidal thoughts and non-suicidal self-harm in a general population sample.

Method
A total of 14 949 adults who completed the 2007 or 2014 Adult Psychiatric Morbidity Survey were included. We measured alcohol use with the Alcohol Use Disorders Identification Test (AUDIT). Domains of alcohol use relating to risk categories, weekly consumption, binge drinking, dependence symptoms, harmful effects and concern from others were derived from relevant AUDIT items. Self-reported past year suicide attempt, suicidal thoughts and non-suicidal self-harm were measured with the Clinical Interview Schedule, Revised.

Results
We found a linear association between total AUDIT score and outcomes. Three of six specific domains of alcohol use (dependence symptoms, harmful effects of drinking and binge drinking) were associated with increased odds of all three outcomes. There was no association of outcomes with the other domains of alcohol use.

Conclusions
We found evidence of a linear association between total AUDIT score and suicide attempt, suicidal thoughts and non-suicidal self-harm in a representative English general population sample. Our analyses suggest that where alcohol use significantly disrupts day-to-day functioning, this may underpin the relationship between alcohol use and suicide-related outcomes to a greater extent than higher alcohol consumption. Longitudinal research is needed to further understand these relationships.
Tumor-induced Osteomalacia: A Comprehensive Review
Authors: Minisola Salvatore , Fukumoto Seiji , Xia Weibo , Corsi Alessandro , Colangelo Luciano , Scillitani Alfredo , Pepe Jessica , Cipriani Cristiana , Thakker Rajesh V 
Published: November 2022

AbstractTumor-induced osteomalacia (TIO) is an ultrarare paraneoplastic syndrome due to overproduction of fibroblast growth factor 23 (FGF23), with profound effects on patient morbidity. TIO is an underdiagnosed disease, whose awareness should be increased among physicians for timely and proper management of patients. Symptoms reported by patients with TIO are usually nonspecific, thus rendering the diagnosis elusive, with an initial misdiagnosis rate of more than 95%. Biochemical features of TIO are represented by hypophosphatemia, increased or inappropriately normal levels of FGF23, and low to low normal circulating 1,25-dihydroxyvitamin D (1,25(OH)2D). Phosphaturic mesenchymal tumors are the pathological entities underlying TIO in most affected patients. There is now evidence that FN1-FGFR1 and FN1-FGF1 fusion genes are present in about half of tumors causing this paraneoplastic syndrome. Tumors causing TIO are small and grow slowly. They can occur in all parts of the body from head to toe with similar prevalence in soft tissue and bone. There are a number of functional and anatomical imaging techniques used for tumor localization; 68Ga DOTA-based technologies have better sensitivity. Surgery is the treatment of choice; several medical treatments are now available in case of inability to locate the tumor or in case of incomplete excision.
Mechanobiology of the cell wall – insights from tip-growing plant and fungal cells
Authors: Municio-Diaz Celia , Muller Elise , Drevensek Stéphanie , Fruleux Antoine , Lorenzetti Enrico , Boudaoud Arezki , Minc Nicolas 
Published: November 2022

ABSTRACT
The cell wall (CW) is a thin and rigid layer encasing the membrane of all plant and fungal cells. It ensures mechanical integrity by bearing mechanical stresses derived from large cytoplasmic turgor pressure, contacts with growing neighbors or growth within restricted spaces. The CW is made of polysaccharides and proteins, but is dynamic in nature, changing composition and geometry during growth, reproduction or infection. Such continuous and often rapid remodeling entails risks of enhanced stress and consequent damages or fractures, raising the question of how the CW detects and measures surface mechanical stress and how it strengthens to ensure surface integrity? Although early studies in model fungal and plant cells have identified homeostatic pathways required for CW integrity, recent methodologies are now allowing the measurement of pressure and local mechanical properties of CWs in live cells, as well as addressing how forces and stresses can be detected at the CW surface, fostering the emergence of the field of CW mechanobiology. Here, using tip-growing cells of plants and fungi as case study models, we review recent progress on CW mechanosensation and mechanical regulation, and their implications for the control of cell growth, morphogenesis and survival.
Interventions aiming to eliminate catastrophic costs due to tuberculosis: a protocol for a systematic review and meta-analysis
Authors: Carballo-Jimenez Paula P. , Datta Sumona , Aguirre-Ipenza Rubén , Saunders Matthew J. , Quevedo Cruz Luz , Evans Carlton A. 
Published: November 2022

Background: People with tuberculosis disease and their household members may suffer direct out-of-pocket expenses and indirect costs of lost income. These tuberculosis-related costs can worsen poverty, make tuberculosis treatment completion unaffordable, impair quality of life and increase the risk of death. Costs due to tuberculosis are usually defined as catastrophic if they exceed 20% of the pre-disease annual household income. The World Health Organisation strategy to “End TB” and the United Nations Sustainable Development Goals include the target that no households should face catastrophic costs due to tuberculosis. However, there is limited evidence and policy concerning how this global priority of eliminating catastrophic costs due to tuberculosis should be achieved. This systematic review and meta-analysis aims to address this knowledge gap. Methods: Publications assessing interventions that aimed to eliminate catastrophic costs will be identified by searching three electronic databases (PubMed, Scopus and Web of Science) together with reference lists from pertinent publications. We will screen eligible studies, extract data, and assess the risk of bias with the quality assessment tool from the National Heart, Lung, and Blood Institute. Discrepancies will be resolved by discussion between the reviewers. If we find sufficient comparable studies quantifying strategies to eliminate catastrophic costs then a meta-analysis will be performed. This systematic review and meta-analysis is registered with the PROSPERO database (CRD42022292410). Conclusion: This systematic review and meta-analysis aims to rigorously assess the evidence for strategies to eliminate catastrophic costs due to tuberculosis.
The association between taking a death certificate course and practical knowledge about death certificate among Saudi medical students
Authors: Aljerian Khaldoon , AlHogail Norah , Alaqeel Saud , Alderaan Khayyal , Al-hawamdeh Abdulla , Alhudhaif Hani 
Published: November 2022

In Saudi Arabia, family members, general practitioners, morticians, or police officer, who are eligible to request autopsies, refrain from doing so to expedite the burial of the deceased. Inaccurate entries into the medical death certificate facilitate so. The reason for ignoring the completion of death certificates is not fully explored. Yet, incompetence or avoiding medicolegal allegations seem to be causative factors. In this study, we conducted a cross-sectional study on 335 medical students from all years of medical school to determine the association between taking the death certificate course and the level of knowledge about death certificates among medical students. The results showed an increase in the level of knowledge of students who took the course compared to the students who did not. Among the students who have seen death certificates, the majority have not seen enough number of death certificates. Cultural mores of the medical students are expected to govern their carefulness in filling out the death certificates.
MUSEU CIÊNCIA E VIDA E OS VÍNCULOS ESTABELECIDOS DURANTE A PANDEMIA
Authors: Dahmouche Mônica Santos , Pinto Simone Pinheiro 
Published: November 2022

A pandemia da Covid-19 provocou um movimento disruptivo na sociedade global. O rápido processo de mudança imposto tornou urgente a adoção de medidas alternativas para manter o contato com o público visitante, novas parcerias foram criadas e caminhos alternativos que estavam no horizonte para desenvolvimento futuro foram rapidamente implementados, como por exemplo o uso do espaço virtual dos museus, para muito além dos sites. Neste texto apresentamos um programa de produção de conteúdo digital, o CONECTA MUSEU CIÊNCIA E VIDA, que tem como intuito promover a conexão com o público visitante por meio de lives sobre temas que estejam em debate ou tenham conexão com aqueles abordados no museu. A série CONECTA tornou as redes sociais do museu, especialmente o canal no YouTube mais robusta, com mais seguidores. Neste relato, fizemos um recorte dos treze primeiros episódios, e discutimos a reverberação junto ao público, assim como a receptividade da série, sob a ótica do alcance e da relação dialógica estabelecida, além das perspectivas futuras.
FORMAÇÃO DE PROFESSORES EM UNIÃO DOS PALMARES PARA A UTILIZAÇÃO DO GOOGLE SALA DE AULA
Authors: Novais Juliana Oliveira de Santana , Nascimento Maria Edna Porangaba do , Dias Poliana Gomes 
Published: November 2022

O presente trabalho tem como objetivo entender a percepção dos professores sobre a formação recebida pela Secretaria Municipal de Educação da cidade de União dos Palmares – AL para a utilização do Google sala de aula durante a pandemia. O distanciamento social provocado pela Covid-19, reconfigurou as relações sociais levando os profissionais da educação e estudantes a um novo processo de ensino-aprendizagem. Dessa forma, utilizando-se da abordagem qualitativa, o trabalho recorreu a um questionário semiestruturado, aplicado a 21 (vinte e um) professores do ensino fundamental anos finais, participantes da formação. Buscamos com o questionário compreender como se deu a formação e o que os professores têm a dizer sobre a formação. Os resultados demonstraram que apesar do curso de formação ter acontecido em um curto prazo, os professores disseram ter sido fundamental para poder lidar com o momento de distanciamento social.
ALCOOLISMO E POBREZA EM “ENSAIO SOBRE A CRIMINALIDADE DA MULHER NO BRASIL”
Authors: De Paula Éder Mendes , Souza Raick De Jesus 
Published: November 2022

O objetivo central deste artigo é analisar as inter-relações estabelecidas por Nise da Silveira, em sua tese, acerca da criminalidade da mulher com o alcoolismo e a pobreza. Para tal empreendimento de pesquisa, mobilizaremos os principais argumentos e autores acionados pela médica no tratamento do tema da criminalidade, colocando em relevo suas concepções sobre fatores individuais e sociológicos. Veremos brevemente a divulgação de seus resultados por meio de uma de suas participações na imprensa, a fim de melhor percebermos seu engajamento no debate público sobre o que era ser mulher ou mulher criminosa no Brasil da década de 20 do século passado. Desejamos com este artigo contribuir com as investigações que analisaram o início do percurso formativo de Nise da Silveira e suas percepções sobre a criminalidade da mulher brasileira.
TÁ DE GRAÇA, SUBURBANO? REPRESENTAÇÕES DO SUBÚRBIO CARIOCA COMO PRODUTO TELEVISIVO
Authors: Araujo Victor Marques de , Osorio Patricia Silva 
Published: November 2022

O objetivo deste trabalho é buscar compreender os significados elencados pelo programa de televisão Tô de Graça, produzido pelo canal Multishow, em sua construção sobre o subúrbio carioca, destacando-se o conceito de suburbania. Realizar-se-á uma análise do episódio de estreia do produto televisivo tendo como base as representações do subúrbio já lançadas pelo episódio em si e a entrevista com um membro do elenco, além da observação específica das relações estabelecidas pelos personagens na investigação de suas ações no episódio, que culminam na composição de sua relação com a hierarquia da dinâmica social e na representação social subúrbio. Justifica-se, assim, academicamente o tema pela relação estrita da ideia de suburbania com temas mais amplos como hierarquia social e relação simbólica entre classes sociais.
NARRATIVAS, FOLHETOS E CULTOS: PERSPECTIVAS SERTANEJAS
Authors: Cavignac Julie Antoinette 
Published: November 2022

A pesquisa realizada no interior do Rio Grande do Norte procura entender como se organizam as representações da história, do mundo sobrenatural e das práticas religiosas. O exame das crenças e práticas, graças à análise das narrativas, revela que o real e o imaginário se misturam: o discurso da vida cotidiana e os registros míticos referem-se a santuários, casas assombradas e serras repletas de figuras humanas, assombrações, animais e fenômenos mágicos vindo de um tempo primordial. Da mesma forma, a análise da santificação revela que a humanidade e o sofrimento dos santos são elementos fundadores do sistema de representação do mundo divino. É possível expandir esta observação para o caso de mortes dolorosas e solitárias, se considerarmos o destino das almas. Ao examinar as narrativas orais e os folhetos de cordel, notamos a presença de figuras locais cujo sofrimento ou vida exemplar foi transformada numa santificação. O foco deste trabalho será explorar as complexas relações que existem entre os três níveis, humano, natural e sobrenatural.
BIOTECNOLOGIA E CONSTRUÇÃO DE SI
Authors: Russo Jane 
Published: November 2022

Neste artigo discutimos a constituição da saúde como um imperativo moral e suas transformações. Analisamos as mutações do campo médico a partir do florescimento e da disseminação de biotecnologias (aí incluídos os medicamentos) para os mais diversos fins. A partir de autores como Nikolas Rose e Jeremy Green apontamos o modo como tal disseminação tem contribuído para borrar as fronteiras entre tratamento e aprimoramento/otimização de si através do corpo. Trazemos como exemplo um estudo de caso: o lançamento do medicamento Viagra para “disfunção erétil”. Discutimos como o diagnóstico “disfunção erétil”, que substitui a categoria “impotência”, tem várias vantagens sobre esta, a maior delas sendo a possibilidade de uma gradação da disfunção. Argumentamos que a definição bastante alargada de disfunção leva ao surgimento de um ideal de desempenho sem falhas, de tal modo que o deslizamento entre tratar uma disfunção e otimizar uma função torna tratamento e aprimoramento eventos indistinguíveis.
Unilateral Pulmonary Fibrosis with Pulmonary Artery Agenesis (PAA): A Case Report
Authors: Di Frega G. Scotto 
Published: November 2022

Unilateral pulmonary artery agenesis (AUAP) is a rare clinical condition that can be seen either in isolation or in combination with other congenital vascular disorders. When isolated, the patient may be almost asymptomatic and only become aware of this anomaly through medical examinations performed for other reasons.
Here we discuss the case of a female patient with chest CT evidence of unilateral fibrous thickening, with pulmonary hypoexpansion; investigations revealed the coexistence of AUAP.
This clinical association is described by very rare case-reports in the literature.
Strategy to avoid vascular injuries in revision total hip arthroplasty with intrapelvic implants
Authors: Diesel Cristiano V. , Guimarães Marcelo R. , Menegotto Samuel M. , Pereira Adamastor H. , Pereira Alexandre A. , Bertolucci Leonardo H. , Freitas Eduarda C. , Galia Carlos R. 
Published: November 2022

Aims Our objective was describing an algorithm to identify and prevent vascular injury in patients with intrapelvic components. Methods Patients were defined as at risk to vascular injuries when components or cement migrated 5 mm or more beyond the ilioischial line in any of the pelvic incidences (anteroposterior and Judet view). In those patients, a serial investigation was initiated by a CT angiography, followed by a vascular surgeon evaluation. The investigation proceeded if necessary. The main goal was to assure a safe tissue plane between the hardware and the vessels. Results In ten at-risk patients undergoing revision hip arthroplasty and submitted to our algorithm, six were recognized as being high risk to vascular injury during surgery. In those six high-risk patients, a preventive preoperative stent was implanted before the orthopaedic procedure. Four patients needed a second reinforcing stent to protect and to maintain the vessel anatomy deformed by the intrapelvic implants. Conclusion The evaluation algorithm was useful to avoid blood vessels injury during revision total hip arthroplasty in high-risk patients. Cite this article: Bone Jt Open 2022;3(11):859–866.
Optical weak measurement for the precise thickness determination of an ultra-thin film
Authors: Zhao Jing-Ru , Wu Zhen-Jie , Wang Gui-Qiang , Wang Chun-Nan , Deng Bo-Fu , Sun Shu-Qing 
Published: November 2022

A total internal reflection system based on the weak value amplification principle is set up for the precise measurement of the thickness of an ultra-thin film. In this system, the film thickness is derived from the change of the double-peak pointer caused by the effective refractive index of the film, which is correlated to its thickness. The sensitivity and resolution of this system reached 2727.21 nm/RIU and

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, respectively, determined by using a sodium chloride solution with a refractive index of 1.331911. The growth process of TA/Fe(III) assembled films with thicknesses in the few nanometers range is monitored using the as-set-up system, and the experimental results are consistent with a theoretical calculation based on the Maxwell Garnett effective medium. Additionally, we theoretically calculated the detection limit for the thickness measurement of the film as 22 pm. We clearly provide a potential method for the precise measurement of the thickness of an ultra-thin film.
Knowledge, practices and perceptions of communities during a malaria larviciding randomized trial in the city of Yaoundé, Cameroon
Authors: Ngadjeu Carmène Sandra , Talipouo Abdou , Kekeunou Sévilor , Doumbe-Belisse Patricia , Ngangue-Siewe Idriss Nasser , Djamouko-Djonkam Landre , Kopya Edmond , Bamou Roland , Sonhafouo-Chiana Nadège , Nkahe Leslie , Njuabe Metoh Theresia , Awono-Ambene Parfait , Wondji Charles Sinclair , Antonio-Nkondjio Christophe 
Published: November 2022

Background
Urban malaria is becoming a major public health concern in major cities in Cameroon. To improve malaria vector control, a pilot larviciding trial was conducted to assess its impact on mosquito density and malaria transmission intensity in Yaoundé. The present study investigated perceptions and practices of communities on malaria control during the larviciding trial implemented in Yaoundé.

Methods
Quantitative and qualitative data were collected in non-intervention and intervention areas. Quantitative data were collected during three cross-sectional surveys using a structured pre-tested questionnaire while qualitative data were obtained through interviews. A total of 26 in-depth interviews and eight focus group discussions with community members were performed. A binary logistic regression model was used to assess the perception of the community on larviciding impact on some malaria or bed nets use indicators.

Results
People living in intervention areas were 2.64 times more likely to know the mode of malaria transmission (95% CI: 1.82–3.84; p<0.001) and 1.3 time more likely to know mosquito breeding habitats (95% CI: 1.06–1.56; p = 0.009) compared to those living in non-intervention areas. In intervention areas, interviewee opinions on larviciding were generally good i.e. most interviewees reported having noticed a reduction in mosquito nuisance and malaria cases following larviciding implementation; whereas in non-intervention areas no report of reduction of mosquito nuisance was recorded. LLINs were regularly used by the population despite the implementation of larviciding treatments. There was high interest in larviciding program and demand for continuation, even if this needs the community involvement.

Conclusion
The larviciding program in the city of Yaoundé did not negatively affected community members’ behaviour and practices concerning the use of treated nets. The study indicated the acceptance of larviciding program by the population. This positive environment could favour the implementation of future antilarval control activities in the city of Yaoundé.