bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mega-analysis of brain structural covariance, genetics, and clinical phenotypes
Authors: Wen Junhao , Nasrallah Ilya , Abdulkadir Ahmed , Satterthwaite Theodore , Erus Guray , Robert-Fitzgerald Timothy , Singh Ashish , Sotiras Aristeidis , Boquet-Pujadas Aleix , Yang Zhijian , Mamourian Elizabeth , Doshi Jimit , Cui Yuhan , Sriniva Dhivya , Bergman Mark , Bao Jingxuan , Veturi Yogasudha , Zhou Zhen , Yang Shu , Dazzan Paola , Kahn René , Schnack Hugo , Zanetti Marcus , Meisenzahl Eva , Busatto Geraldo , Crespo-Facorro Benedicto , Pantelis Christos , Wood Stephen , Zhuo Chuanjun , Shinohara Russell , Gur Ruben , Gur Raquel , Koutsouleris Nikolaos , Wolf Daniel H. , Saykin Andrew J. , Ritchie Marylyn , Shen Li , Thompson Paul , Colliot Olivier , Wittfeld Katharina , Grabe Hans , Tosun Duygu , Bilgel Murat , An Yang , Marcus Daniel , LaMontagne Pamela , Heckbert Susan , Austin Thomas , Launer Lenore , Espeland Mark , Masters Colin , Maruff Paul , Fripp Jurgen , Johnson Sterling , Morris John , Albert Marilyn , Bryan Nick , Resnick Susan , Fan Yong , Habes Mohamad , Wolk David , Shou Haochang , Davatzikos Christos 
Published: April 2022

Abstract
Normal and pathologic neurobiological processes influence brain morphology in coordinated ways that give rise to structural covariance patterns across brain regions and individuals. We present a mega-analysis of structural covariance with magnetic resonance imaging of 50,699 healthy and diseased individuals (12 studies, 130 sites, and 12 countries) over their lifespan (ages 5 through 97). Patterns of structural covariance (PSC) were highly heritable (0.05< h2 <0.78) and significantly associated with 1610 independent significant variants after Bonferroni correction (10.3 > -log10[p-value] > 8.8): 1245 previously unreported, and 69% of them independently replicated (-log10[p-value] = 4.5). Associations revealed an imaging phenotypic landscape between 2003 PSCs and 49 clinical and cognitive traits at multiple scales. We constructed machine learning-derived individualized imaging signatures for various disease diagnoses using PSC features at multiple scales, suggesting that disease effects on the brain were better manifested in a multi-scale continuum than on any single scale. Experimental results were integrated into the Multi-scale Structural Imaging Covariance (MuSIC) atlas and made publicly accessible through the BRIDGEPORT web portal (https://www.cbica.upenn.edu/bridgeport/). Our results reveal strong associations between brain structural covariance, genetics, and clinical phenotypes, supporting that PSCs can serve as an endophenotypic anatomic dictionary in future research.
NRF2 signaling is repressed in HPV-driven head and neck cancer and correlates with better prognosis: HPV-E6/E7 confers chemosensitivity by inhibiting NRF2
Authors: Ramesh Pushkal Sinduvadi , Bovilla Venugopal Reddy , Swamy Vikas , Manoli Nandini Nandish , Dasegowda Krishnappa , Siddegowda Siddegowda , Chandrashekarappa Shilpa , Somasundara Vishveshwara , Devegowda Devanand , Thimmulappa Rajesh 
Published: April 2022

Abstract
Background: To investigate the role of NRF2 signaling in conferring superior prognosis in patients with HPV positive (HPV+ve) head & neck squamous cell carcinomas (HNSCC) compared to HPV negative (HPV-ve) HNSCC and develop molecular markers for selection of HPV+ve HNSCC patients for treatment de-escalation trials. Methods: We compared NRF2 activity (NRF2, KEAP1, and NRF2-transcriptional targets), p16, and p53 levels between HPV+ve HNSCC and HPV-ve HNSCC in prospective and retrospective tumor samples. Cancer cells were transfected with HPV E6/E7 plasmid to elucidate if HPV infection repress NRF2 activity and sensitize to an anticancer drug. Results: Prospective analysis revealed a marked reduction in expression of NRF2, and its target antioxidant genes (NQO1, HMOX-1, GCLC, and GCLM) in HPV+ve tumors compared to HPV-ve tumors. A retrospective analysis by IHC revealed significantly lower NQO1 in p16high tumors compared to p16low tumors and the NQO1 expression correlated negatively with p16 and positively with p53. Analysis of the TCGA database confirmed low constitutive NRF2 activity in HPV+ve HNSCC compared to HPV-ve HNSCC and revealed that HPV+ve HNSCC patients with ‘low NQO1’ expression showed better overall survival compared to HPV+ve HNSCC patients with ‘high NQO1’ expression. Ectopic expression of HPV E6/E7 plasmid in cancer cells repressed constitutive NRF2 activity, increased KEAP1, reduced total GSH levels, and enhanced cytotoxic effects of cisplatin. Conclusions: Low constitutive NRF2 activity contributes to better prognosis in patients with HPV+ve HNSCC compared to HPV-ve HNSCC. Co-expression of p16high, NQO1low, and p53low could serve as a predictive biomarker for the selection of HPV+ve HNSCC patients for de-escalation trials.
Bioequivalence study of a new fixed-dose combination tablet containing irbesartan and hydrochlorothiazide in healthy volunteers
Authors: Uzunova Katya , Petrov Andrey , Gatchev Emil , Pavlova Velichka , Filipova Elena , Jiménez Cintia , Kalinov Krassimir , Vekov Toni 
Published: April 2022

Abstract
Background and objective:
The combination antihypertensive therapy has shown greater blood pressure lowering potential as well as better adherence to the treatment. Therefore, a new generic fixed-dose combination containing irbesartan and hydrochlorothiazide was developed and rate and extend of absorption were compared with reference formulation to prove its bioequivalence in healthy volunteers.
Methods
A single-center, open-label, randomized, two-period, crossover, single dose study under fasting conditions, including at least 7-days washout period, was performed in 30 healthy male and female volunteers. Twenty blood samples were collected in each study period: prior to dosing (0:00) and up to 72:00 hours after dosing and plasma concentrations of irbesartan and hydrochlorothiazide were analyzed using preliminary validated methods.
Results
The 90% CIs for the geometric mean ratios of test and reference of Cmax and AUC0 − t were 89.22–98.80% and 100.58–115.11%, respectively, for irbesartan and 91.80–122.96% and 94.88–117.56%, respectively. Thus, the corresponding ratios of Cmax and AUC0 − t for irbesartan and hydrochlorothiazide met the predetermined criteria for bioequivalence (90% confidence intervals of the geometric mean ratios of test and reference within the 80.00% − 125.00%). Both the test and reference products demonstrated good tolerability profile in this population, and no serious AEs were observed.
Conclusions
Therefore, the test product (Irbesartan/hydrochlorothiazide 300/25 mg film-coated tablets, manufactured by Tchaikapharma High Quality Medicines Inc., Bulgaria) and the reference product (CoAprovel 300/25 mg film-coated tablets, manufactured by Sanofi Clir SNC, France) are considered bioequivalent.
Impact of Land Use Land Cover Change using Remote Sensing with Integration of Socio-Economic Data on rural Livelihoods in case of Nashe Watershed, Ethiopia
Authors: Fikadu Gelana 
Published: April 2022

Abstract

Land use/land cover is an important component in understanding the interactions of human activities with the environment and is necessary to recognize the changes in order to monitor and maintain a sustainable environment. This study aimed to analyze changes in land use and land cover spatially and temporally in the Nashe Watershed. since the dam of the Nashe watershed was built in 2012 socio-economic characteristics of the area are used to interpret the causes of land use and land cover changes occurring changes on their life and environment. The socioeconomic data were analyzed with Excel and integrated with biophysical data. For the 2010–2020 ten-year period showed that Cropland and Woodland have been reduced from 73–62% and 18–14%, respectively, and Wetlands have been fully converted to Water Bodies, alternately increasing Water Bodies and Pasture Land from 43.9–54.5% and 0.04–18%. respectively the reason for this discussion of change was the construction of dams without considering the effect on livelihood and the environment. The government could not give these people adequate compensation for their lands conquered by water. These peoples were forced to expand their agricultural lands by cutting and logging trees in order to make a living to get basic needs. Therefore, Nashe watershed is identified as an area highly affected by land use and land cover change and it is necessary to closely monitor land use/land cover to maintain a sustainable environment for future
Temperature measurement point optimization and experimental research for Bi-rotary Milling Head of Five-axis CNC Machine Tool
Authors: Dai Ye , Li Yang , Li Zhaolong , Wen Wanjian , Zhan Shiqiang 
Published: April 2022

Abstract
Thermal deformation is the main factor affecting the machining accuracy of the Bi-rotary Milling Head. To accurately find out the temperature-sensitive points of the Bi-rotary Milling Head to suppress thermal deformation, this paper adopts the BP neural network sensitivity analysis method with improved connection weights to optimize the temperature measurement points, and the analysis results are subjected to randomized mean value processing to reduce the randomness of the initialization of the prediction model. The number of temperature measurement points is reduced from 15 to 4. Taking the 5AS01 Direct-drive Bi-rotary Milling Head as an example, a thermal-structural coupling model is established to analyze its thermal characteristics, and the capillary copper tube cooling suppression experiment is arranged according to the position of the temperature-sensitive points. The experimental results show that cooling the temperature-sensitive points can simultaneously reduce the thermal error in X and Z-directions by about 58%, providing a basis for the Bi-rotary Milling Head to improve machining accuracy.
Tomentosin induces apoptosis in pancreatic cancer cells through increasing reactive oxygen species and decreasing mitochondrial membrane potential
Authors: Güçlü Ebru , Ayan İlknur Çınar , Dursun Hatice Gül , Vural Hasibe 
Published: April 2022

Abstract
The aim of this study was to determine possible anticancer effect of tomentosin, a natural sesquiterpene lactone, on pancreatic cancer cells. The cytotoxic effect of tomentosin on human pancreatic cancer cells was determined by XTT analysis. After treatment with tomentosin, apoptosis rate was determined and migration, invasion and colony formation analyzes were performed. Reactive oxygen species (ROS) level and the change in mitochondrial membrane potential (MMP) were evaluated. The effect of tomentosin on expression levels of apoptosis-related genes was determined by qRT-PCR. The levels of Caspase-3 and Caspase-9 were analyzed by western blot. The IC50 dose of tomentosin was found to be 31.11 µM in PANC-1 cells and 33.93 μM in MIA PaCa-2 cells for 48 hours. Treatment with tomentosin induced apoptosis and ROS production in pancreatic cancer cells. And, after tomentosin treatment, MMP decreased and migration, invasion and colony formation of pancreatic cancer cells were suppressed. While tomentosin affected expression level of apoptosis-related genes, an increase was observed in Caspase-3 and Caspase-9 protein levels. Considering all the data together, this study reveals that tomentosin has anticancer effects on pancreatic cancer cells, and therefore it predicts that tomentosin can be evaluated as an effective agent against pancreatic cancer.
Spatiotemporal inter-comparison of satellite precipitation products over India
Authors: Kumar Brijesh , Roy Dipankar , Lakshmi Venkataraman 
Published: April 2022

Abstract
RegionSatellite Precipitation Products (SPPs) were statistically evaluated for their usefulness at different spatial, temporal, seasonal, altitude, and rainfall intensities scales over India.Study FocusThe study evaluates the performance of World Meteorological Organization (WMO) Space-based Weather and Climate Extremes Monitoring Demonstration Project (SEMDP) Global Satellite Mapping of Precipitation (GSMaP) Gauge Adjusted Near Real Time Product (GNRT), Integrated Multi-satellitE Retrievals for GPM (IMERG) and Climate Prediction Center morphing method (CMORPH) for three years (2015-2017) using daily gridded gauge data from Indian Meteorological Department (IMD). The statistics used are visual interpretation (histograms, CDF plots), yes/no dichotomous [(Bias score (B), probability of detection (POD), false alarm ratio (FAR), probability of false alarm detection (POFD), accuracy (ACC), threat score (TS) and skill score (SS)] and continuous variable verification statistics [correlation coefficient (CC), relative bias (RB), mean absolute difference (MAD) and root mean square error (RMSE)]. New Meteorological Insights for the RegionThe study found that GNRT is better than other SPPs at different temporal, spatial, altitudinal and rainfall frequency bands. The spatial variability of the metrics are similar but maximum CC >0.8 and least RMSE <10mm was recorded for GNRT over Central India. Additionally, all SPPs underestimate the precipitation amount in the Northeast and overestimate the same in Southern India. The precipitation detection score is good except in the Himalayan foothills and the Southern coast. All the SPPs are able to represent well the CDF for the precipitation intensities. Among all SPPs, the elevation has the least impact on GNRT, however significant elevation error has been found for CMROPH. At first glance, the gauge adjusted GNRT and IMERG_F perform better than the other SPPs. The results of the study can be utilized for the disaster risk and hydrological modelling and irrigation system analysis in the rain gauge data poor regions of the country.
GC-MS profiling of the phytochemical constituents of the whole plant of Brillantaisia owariensis (P. Beauv) and its anti-inflammatory prospects in the management of African Trypanosomiasis
Authors: Oniye Sonnie Joshua , Ayawa Nafisa Garba , Mazlan Mazlina , Ramon-Yusuf Suleiman Babatunde , Wada Yunusa Adamu , Shehu Dalhatu Mukhtari 
Published: April 2022

Abstract
Brillantaisia owariensis (Acanthaceae) is evident in its traditional use in the forest region of West Africa for the treatment of several ailments, including trypanosomiasis. Despite its widespread use, the plant has not been subjected to pharmacological investigations to ascertain its phytochemical profile and efficacy in folklore medicine. This study was designed to determine the phytochemical and GC-MS profile of the whole plant of B. owariensis and elucidate its pharmacological implications in managing trypanosomiasis. The plant was collected from Nigeria's South-western Forest vegetation zone. Thirty grams of the processed powder was extracted using the Soxhlet method using methanol as solvent. The phytochemical and Gas Chromatography-Mass Spectroscopy analyses of the methanol extract were done. Thirty BALB/c mice were randomly allocated into six groups of five mice to test for anti-inflammatory activity. Each mouse in groups 2–6 were infected with 0.1 mL of 106 T. brucei /mL, while group 1 served as neutral non-infected control. Following the establishment of parasitaemia by 3 days post-infection, mice were either non-treated, treated with standard drug or treated at varying dosages of methanol extracts and sacrificed by day 9 for histopathologic evaluation. High levels of Phyto-constituents were detected, and the GC-MS profiling revealed the presence of twenty-four bioactive compounds. The extracts of B. owariensis revealed ameliorative and anti-inflammatory activities on the histology of the vital organs and tissues at varying dosages. The methanol extract of B. owariensis is rich in bioactive components with promising potential in managing inflammatory diseases such as African trypanosomosis.
A strangely light neutron star
Authors: Doroshenko Victor , Suleimanov Valery , Pühlhofer Gerd , Santangelo Andrea 
Published: April 2022

Abstract
To constrain the equation of state of cold dense matter, astrophysical measurements are essential. These are mostly based on observations of neutron stars in the X-ray band, and, more recently, also on gravitational wave observations. Of particular interest are observations of unusually heavy or light neutron stars which extend the range of central densities probed by observations and thus permit testing nuclear physics predictions over a wider parameter space. Here we report on the analysis of such a star, a central compact object within the supernova remnant HESS J1731-347. We estimate the mass and radius of the neutron star to be M = 0.77(20)Msun and R = 10.4(8) km, respectively, based on modeling of the X-ray spectrum and a robust distance estimate from Gaia observations. Our estimate implies that this object is either the lightest neutron star known, or a "strange star” with a more exotic equation of state. Adopting a standard neutron star matter hypothesis significantly constrains the corresponding equations of state.
Alkaline protease from Bacillus cereus was characterized and optimized for eco-friendly degradation of feathers
Authors: Masi Chandran , Gemechu Getachew , Tafesse Mesfin 
Published: April 2022

Abstract
Purpose: To investigate the detailed analysis of alkaline protease production, media optimization, protease assay, mass production, and application in feather degradation by isolates of Bacillus cereus strain isolated from leather industry effluent, Ethiopia.Methods: In this study, media optimization was subjected to nine different parameters like fermentation time, temperature, pH, Substrate concentration, carbon sources, nitrogen sources, metal ions, Inoculum size, and sodium chloride concentration to check the maximum alkaline protease production using Bacillus cereus. Ammonium sulfate and dialysis were used to partially purify the enzyme from Bacillus cereus. SDS-PAGE was used to test the activity and total protein content of the partially purified enzymes, and then extracellular alkaline protease from Bacillus cereus was used to examine hydrolyzed chicken feathers, and the findings were reported.Results: The maximum enzyme activity of crude alkaline protease from Bacillus cereus was achieved at optimized conditions. The specific enzyme activity of partially purified alkaline protease after dialysis (123.35U/mg) showed high activity compared to the crude protease (24.45U/mg). Finally, the partially purified enzyme was tested for its potential to degrade feather waste. Partially purified enzyme demonstrated significant feather degradation of 76.5% on the fifth day at optimized temperature and enzyme concentration.Conclusions: Bacillus cereus was found to cause considerable feather deterioration in this investigation. It's was also been discovered that enzyme activity gets enhanced at a specific optimized condition. Compared to the crude enzyme, partially purified and dialyzed enzymes showed considerable change in the enzyme activity, indicating that they have the potential to break down feathers.
Efficacy of intravenous immunoglobulin for Critically Ill Patients With COVID-19: A meta and trial sequential analysis
Authors: Liu Yao , Wu Zongsheng , Zhang Qiuling , Liu Hengjun , Wang Wei , Tong Hanwen , Jiang Yunfei , He Fei , Xu Peng 
Published: April 2022

Abstract
Background: A substantial portion of patients infected with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can progress to critical illness which is associated with high mortality, and prolonged length of hospital stay. Currently, intravenous immunoglobulin (IVIG) therapy is reported widely in these specific population globally. However, the impact of IVIG treatment on clinically relevant outcomes in the critically ill patients with COVID-19 still remained controversial.Methods: The major databases including PubMed, Embase and Cochrane Central Register were searched from January 1, 2019 through January 12, 2022. Studies were limited to severely ill patients with confirmed SARS-CoV-2 infection who receiving IVIG with a comparative group. The primary outcome was the overall mortality. Length of stay (LOS) in the intensive care unit (ICU) and hospital, utilization rate of invasive mechanical ventilation (IMV) and ventilators free days were secondary outcomes. Meanwhile, sensitivity and subgroup analyses, as well as a trial sequential analysis (TSA), were performed.Results: 4 prospective randomized controlled trials (RCT) and 6 retrospective cohort studies (involving 2,054 participants) met the inclusion criteria and were included in our meta-analysis. Compare to standard of care (SOC), the use of IVIG was not associated with decreased odds of death significantly (OR 1.03; 95% CI 0.63–1.67; P = 0.92). No significant difference was detected in either hospital (MD 1.56; 95% CI -1.43–4.55; P = 0.31) or ICU LOS (MD 0.75; 95% CI -0.36–1.86; P = 0.18) between the two groups. A sensitivity analysis revealed that administering IVIG may harmful to patients with moderate to severe ARDS induced by SARS-CoV-2 (OR 2.24; 95% CI 1.09–4.63; P = 0.03). The high level of heterogeneity remained substantial after multiple sensitivity and subgroup analyses were performed. The TSA indicated a lack of sufficient evidence to draw decisive conclusions from the current results since the required information size (RIS) of 8,373 still not reached yet.Conclusions: Current evidence do not support the use of IVIG in treatment for critically ill patients with COVID-19. Further prospective study with well design urgently needed for conclusive findings.
Waste thickness estimation at a large landfill in Brazil from a comparison of simultaneous inversion of different electrode arrays data set
Authors: Lima Victória basileu de oliveira , Guedes Victor Cavalcanti Bezerra , Lima Mirella basileu de oliveira , Borges Welitom Rodrigues , Cunha Luciano Soares da 
Published: April 2022

Abstract
Electrical resistivity tomography (ERT) is particularly suitable for stratigraphic characterization of landfills given its sensitivity to the conductive response of leachate from solid waste. In this context, the simultaneous inversion of data from more than one electrode array can be more reliable than the single inversion of a single set of this data. In this way, resistivity lines were acquired in four electrode arrays at the Jockey Clube landfill in Brazil. We carried out the simultaneous inversion of all possible combinations of the Dipole-dipole (DD), Pole-dipole (PD), Wenner (WN) and Wenner-Shlumberger (WS) arrays and compared the results with direct information from 5 drill holes. The participation of the WN and WS electrode array data obtained similar results when grouped, and the WN and DD matrices, when inverted together, favored the horizontal resolution and the depth of investigation when compared with the inversion of the array separately. To estimate the depth of the waste layer, all available information was used to produce a geoelectric model based on the interpolation of approximate resistivity isovalues at the top and bottom of the waste landfill. The model points to a layer of waste that can vary from 30 to 65 meters in depth, and a total volume of 23,340,429 m³. Finally, the simultaneous inversion of arrays can be a useful tool for landfill investigation, especially if the geoelectric models of the matrices are quite different from each other, in which the subsurface structure is complicated with little a priori information.