bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long noncoding RNA LINC00930 promotes PFKFB3-mediated tumor glycolysis and cell proliferation in nasopharyngeal carcinoma
Authors: He Baoyu , Pan Hongli , Zheng Fengque , Chen Saiqiong , Bie Qingli , Cao Jinghe , Zhao Rou , Liang Jing , Wei Li , Zeng Jianchao , Li Hui , Cui Xing , Ding Yixuan , Chao Wei , Xiang Tiantian , Cheng Yuhe , Qiu Gui , Huang Shishun , Tang Libo , Chang Jiansheng , Luo Delan , Yang Jie , Zhang Bin 
Published: February 2022

Abstract
Background
Metabolic reprogramming is a hallmark of cancer. However, the roles of long noncoding RNAs (lncRNAs) in cancer metabolism, especially glucose metabolism remain largely unknown.

Results
In this study, we identified and functionally characterized a novel metabolism-related lncRNA, LINC00930, which was upregulated and associated with tumorigenesis, lymphatic invasion, metastasis, and poor prognosis in nasopharyngeal carcinoma (NPC). Functionally, LINC00930 was required for increased glycolysis activity and cell proliferation in multiple NPC models in vitro and in vivo. Mechanistically, LINC00930 served as a scaffold to recruit the RBBP5 and GCN5 complex to the PFKFB3 promoter and increased H3K4 trimethylation and H3K9 acetylation levels in the PFKFB3 promoter region, which epigenetically transactivating PFKFB3, and thus promoting glycolytic flux and cell cycle progression. Clinically, targeting LINC00930 and PFKFB3 in combination with radiotherapy induced tumor regression.

Conclusions
Collectively, LINC00930 is mechanistically, functionally and clinically oncogenic in NPC. Targeting LINC00930 and its pathway may be meaningful for treating patients with NPC.
Effect of omega-3 fatty acids on TH1/TH2 polarization in individuals with high exposure to particulate matter ≤ 2.5 μm (PM2.5): a randomized, double-blind, placebo-controlled clinical study
Authors: Wang Xiaomin , Li Shuiqin , Wu Yongcan , Huang Demei , Pei Caixia , Wang Yilan , Shi Shihua , Wang Fei , Wang Zhenxing 
Published: February 2022

Abstract
Background
Long-term exposure to high concentrations of PM2.5 may cause immune system dysfunction and damage to the respiratory and cardiovascular systems. PM2.5 may cause CD4 + T helper cells to polarize toward TH1 or TH2 cell types, which may be associated with the onset and progression of many human diseases. Recent studies have shown that omega-3 fatty acids can regulate human immune function and reduce physiological damage caused by air pollution; however, only limited research has examined the therapeutic effects of omega-3 fatty acids on subjects with high exposure to PM2.5 in mass transit systems such as subways.

Methods
This study was designed as a prospective, randomized, double-blinded (to participants and researchers), placebo-controlled clinical trial. The research plan is to randomly select 120 eligible adults based on the difference in PM2.5 exposure in the Chengdu subway station. They should be aged 20–65 years old and work in the subway station more than or equal to 3 times a week, each time greater than or equal to 8 h, and had worked continuously in the subway station for more than 2 years. All participants will receive omega-3 fatty acids or placebo for 8 weeks. The primary outcomes will be changes in the TH1/TH2 cell polarization index and changes in serum cytokine concentrations. Secondary outcomes will be changes in early indicators of atherosclerosis, pulmonary function, COOP/WONCA charts, and scores on the Short-Form 36 Health Survey for quality of life. Results will be analyzed to evaluate differences in clinical efficacy between the two groups. A 6-month follow-up period will be used to assess the long-term value of omega-3 fatty acids for respiratory and cardiovascular disease endpoints.

Discussion
We will explore the characteristics of the TH1/TH2 cell polarization index in a population with high exposure to PM2.5. Omega-3 fatty acids and placebo will be compared in many ways to test the effect on people exposed to PM2.5 subway stations. This study is expected to provide reliable evidence to support the promotion of omega-3 fatty acids in clinical practice to protect individuals who are highly exposed to PM2.5.

Trial registration
Chinese Clinical Trial Registry ChiCTR2000038065. Registered on September 9, 2020
The “Blood pressure and oxygenation targets in post resuscitation care, a randomized clinical trial”: design and statistical analysis plan
Authors: Kjaergaard Jesper , Schmidt Henrik , Møller Jacob E. , Hassager Christian 
Published: February 2022

Abstract
Background
Comatose patients admitted after resuscitation from cardiac arrest have a significant risk of poor outcome due to hypoxic brain injury. While numerous studies have investigated and challenged the target temperature as the efficacious part of the guideline endorsed Targeted Temperature Management (TTM) protocols, our knowledge and how the remaining parts of the TTM are optimized remain sparse. The present randomized trial investigated two aspects of the TTM protocol: target blood pressure during the ICU stay and oxygenation during mechanical ventilation. Furthermore, the efficacy of device-based post-TTM fever management is addressed.

Methods
Investigator-initiated, dual-center, randomized clinical trial in comatose OHCA patients admitted to an intensive cardiac care unit. Patients are eligible for inclusion if unconscious, older than 18 years of age, and have return of spontaneous circulation for more than 20 min.
Intervention: allocation 1:1:1:1 into a group defined by (a) blood pressure targets in double-blind intervention targeting a mean arterial blood pressure of 63 or 77 mmHg and (b) restrictive (9–10 kPa) or liberal (13–14 kPa) of arterial oxygen concentration during mechanical ventilation. As a subordinate intervention, device-based active fever management is discontinued after 36 h or 72. Patients will otherwise receive protocolized standard of care according to international guidelines, including targeted temperature management at 36 °C for 24 h, sedation with fentanyl and propofol, and multimodal neuro-prognostication. Primary endpoint: Discharge from hospital in poor neurological status (Cerebral Performance category 3 or 4) or death, whichever comes first. Secondary outcomes: Time to initiation of renal replacement therapy or death, neuron-specific enolase (NSE) level at 48 h, MOCA score at day 90, Modified Ranking Scale (mRS) and CPC at 3 months, NT-pro-BNP at 90 days, eGFR and LVEF at 90 days, daily cumulated vasopressor requirement during ICU stay, and need for a combination of vasopressors and inotropic agents or mechanical circulatory support.

Discussion
We hypothesize that low or high target blood pressure and restrictive and liberal oxygen administration will have an impact on mortality by reducing the risk and degree of hypoxic brain injury. This will be assessment neurological outcome and biochemical and neuropsychological testing after 90 days.

Trial registration
ClinicalTrials.gov NCT03141099. Registered on May 2017 (retrospectively registered)
The effect of probiotic cheese consumption on inflammatory and anti-inflammatory markers, disease severity, and symptoms in patients with rheumatoid arthritis: study protocol for a randomized, double-blind, placebo-controlled trial
Authors: Asoudeh Farzaneh , Djafarian Kurosh , Akhalghi Maassoumeh , Mahmoudi Mahdi , Jamshidi Ahmad Reza , Farhadi Elham , Esmaillzadeh Ahmad 
Published: February 2022

Abstract
Background
In recent decades, several studies have shown changes in the intestinal microflora among patients with rheumatoid arthritis (RA). Therapeutic measures using probiotics have shown favorable effects on the recovery of these patients. However, most studies have used probiotic supplements. In this study, we aimed to investigate the effect of probiotic cheese consumption on inflammatory and anti-inflammatory factors, disease severity, and symptoms in these patients.

Methods
This study is a randomized, double-blind clinical trial, in which forty patients with mild to moderate severity of RA will be randomly allocated to receive either 30 g/day probiotic cheese (n = 20) or only low-salt and low-fat cheese without any added probiotic (n = 20) for 12 weeks. Assessment of anthropometric measures and biochemical indicators, including serum concentrations of high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10), will be done at the study baseline and end of the trial. In addition, disease severity and disability will be assessed by DAS-28 and the HAQ-DI questionnaire, respectively.

Discussion
Diet is the leading environmental factor affecting the gut microbiota. A prebiotic-rich diet and probiotics might be beneficial in this regard. To the best of our knowledge, the effect of probiotic supplements on inflammation in these patients has widely been assessed; however, there is only one study that examined the effect of probiotic-containing food in these patients. Further studies are needed to investigate the effect of probiotic-containing foods on inflammatory markers and symptoms in patients with RA.

Trial registration
Iranian Registry of Clinical Trials IRCT20201120049449N1. Registered on 14 February 2021
Tackling the increasing contamination of the water supply by iodinated contrast media
Authors: Dekker Helena M. , Stroomberg Gerard J. , Prokop Mathias 
Published: February 2022

AbstractContrast media are essential for diagnostic and interventional procedures. Iodinated contrast media are the most commonly used agents, with CT requiring the largest overall quantities. Data show that these iodinated contrast media are found in sewage water, surface water and drinking water in many regions in the world. Because standard drinking water purification techniques only provide poor to moderate removal of iodinated contrast media, these substances pose a problem for drinking water preparation that has not yet been solved. There is a growing body of evidence supporting the negative environmental effects of iodinated contrast media via their breakdown products. The environmental impact of iodinated contrast media can be mitigated by measures focusing on the application of contrast media or the excretion of contrast media. Measures with respect to contrast application include reducing the utilization of contrast media, reducing the waste of contrast media and collecting residues of contrast media at the point of application. The amount of contrast media excreted into the sewage water can be decreased by introducing urine bags and/or special urine collection and waste-water processing techniques in the hospital. To tackle the problem of contrast media in the water system in its entirety, it is necessary for all parties involved to cooperate, from the producer of contrast medium to the consumer of drinking water. This paper aims to make health professionals aware of the opportunity to take the lead now in more conscious decisions regarding use of contrast media and gives an overview of the different perspectives for action.
Multiplicity of solutions for an anisotropic variable exponent problem
Authors: Tavares Leandro S. 
Published: February 2022

AbstractIn this manuscript an existence result for an anisotropic variable problem which is related to several applications is proved. By considering suitable hypotheses, the multiplicity of solutions is obtained. Examples of applicability of the results are also presented. The arguments are based on appropriated $L^{\infty }$

L
∞

estimates, sub-supersolutions, and the mountain pass theorem.
Forming a medicines pricing policy for low and middle-income countries (LMICs): the case for Pakistan
Authors: Babar Zaheer-Ud-Din 
Published: February 2022

AbstractEquitable access to medicines has played a vital role to improve patient health outcomes and reducing mortality globally. However, it is important to note that medicines pricing is a key determinant in promoting access to medicines. The studies and empirical data have shown that there are wide variations in prices across countries for the same brand of medicines. World Health Organisation (WHO) has provided guidelines to formulate country pharmaceutical pricing policies. However, little is known how these guidelines will be used in the country-specific setting. This commentary provides guiding principles and outlines the basis to form a medicines pricing policy in a low and middle-income country, Pakistan. It discusses the current medicines pricing policy and provides suggestions for future work. The suggested medicines pricing structure and lessons learned in this commentary can also be applied in other low-resource settings.
Context-dependent influence of threat on honey bee social network dynamics and brain gene expression
Authors: Traniello Ian M. , Hamilton Adam R. , Gernat Tim , Cash-Ahmed Amy C. , Harwood Gyan P. , Ray Allyson M. , Glavin Abigail , Torres Jacob , Goldenfeld Nigel , Robinson Gene E. 
Published: February 2022

ABSTRACT
Adverse social experience affects social structure by modifying the behavior of individuals, but the relationship between an individual's behavioral state and its response to adversity is poorly understood. We leveraged naturally occurring division of labor in honey bees and studied the biological embedding of environmental threat using laboratory assays and automated behavioral tracking of whole colonies. Guard bees showed low intrinsic levels of sociability compared with foragers and nurse bees, but large increases in sociability following exposure to a threat. Threat experience also modified the expression of caregiving-related genes in a brain region called the mushroom bodies. These results demonstrate that the biological embedding of environmental experience depends on an individual's societal role and, in turn, affects its future sociability.
An Organic Quantum Spin Liquid with Triangular Lattice: Spinon Fermi Surface and Scaling Behavior
Authors: Kusamoto Tetsuro , Ohde Chie , Sugiura Shiori , Yamashita Satoshi , Matsuoka Ryota , Terashima Taichi , Nakazawa Yasuhiro , Nishihara Hiroshi , Uji Shinya 
Published: February 2022

Abstract
Quantum spin liquid (QSL) is a novel quantum state of matter, where spins are strongly entangled and highly fluctuating even at very low temperature. In this study, an organic QSL was newly synthesized, and the structure and physical properties were investigated. The antiferromagnetic interaction (J ≈ 24 K) detected between the spins was much smaller than that in the other organic QSLs, while the χ0 and γ values were larger. The results enable significant scalings, γ, χ0 ∝ 1/J for all the organic QSLs, which show the formation of the spinon energy band with the width of J. These findings provide good evidence of the spinon Fermi surface in the QSLs, where the spinon excitation follows the Fermi liquid behavior.
EMI radiation of power transmission lines in Malaysia
Authors: Fikry Azhan , Lim Siow Chun , Ab Kadir Mohd Zainal Abidin 
Published: February 2022

Background: There has been rising concern amongst the public regarding their home's proximity to high tension power transmission lines. The primary cause of fear is the impact of the electromagnetic interference (EMI) radiation on the nearby occupants' health. Despite the presence of national permissible limits of EMI radiation, there is still lack of information with regards to the EMI radiation of the types of power lines configuration in Malaysia. Methods: The electric and magnetic fields of several selected power transmission lines were simulated using the EMFACDC software program from the recommendation ITU-T K.90. Five types of power transmission lines available in Malaysia are considered. Results: It was found that the simulated electric and magnetic field levels at all the power lines' right of way (ROW) boundary complies with the prescribed exposure limit. However, the electromagnetic fields (EMF) level increases significantly as the separation distance is reduced from 30m. For a more conservative approach, the ROW can be set at 30m across all transmission voltage level and corridor area condition. Conclusion: It can be concluded that Malaysia's power transmission lines are within the prescribed exposure limits. To further minimize the electric and magnetic field level, it is recommended that the residential building should be built at least 30 meters away from the power transmission lines, especially for the 275kV double circuit, 275/132kV quadruple circuit, and 500kV double circuit lines.
Case Report: Purtscher-like retinopathy in a patient with lung adenocarcinoma
Authors: Mahmoud Anis , zaghdoudi Asma , Boucharb Soumaya , Abid Fatma , Mbarek Sameh , Ibn Hadj Amor Hassan , Braiek Sawssen , Keskes Boudawara Nadia , knani Jalel , Messaoud Riadh 
Published: February 2022

This case report describes an unreported case of Purtscher-like retinopathy in a patient with pulmonary adenocarcinoma. A 39-year-old man was hospitalized for exploration of a hemoptysis and bilateral blurry vision. Fundoscopic examination showed multiple areas of retinal whitening in the peripapillary area. A chest computed tomography scan then showed a ground glass opacity in the right upper lobe associated to a hilar lymphadenopathy. A thoracotomy and lung biopsy were performed concluding with the diagnosis of lung adenocarcinoma. The patient was treated with high-dose corticosteroids and received Taxol-Carboplatin chemotherapy with good visual outcomes. The article discusses furthermore the importance of including pulmonary adenocarcinoma to the list of systemic conditions for Purtscher-like retinopathy.
Negative opinion on work as variable of career shift among doctors in Poland. Are we running out of doctors in post-COVID times?
Authors: Pruszyński Jacek , Cianciara Dorota , Engel Janusz , Zgliczyński Wojciech 
Published: February 2022

Wprowadzenie i cel pracy. Warunki i ocena pracy, stres, wypalenie zawodowe wśród lekarzy są przedmiotem analiz i badań w Polsce. Mniej uwagi skupia problem odejścia od zawodu. Celem badania było określenie związku między cechami społeczno-demograficzno-zawodowymi lekarzy i subiektywną oceną ich aktualnej pracy a chęcią podjęcia kariery alternatywnej.
Materiał i metody. Przeprowadzono badanie ankietowe wśród 315 lekarzy odbywających kursy w Centrum Medycznym Kształcenia Podyplomowego w roku 2018/19. Kwestionariusz składał się z 14 pytań. Zmienną objaśnianą była deklaracja chęci zmiany zawodu lekarza na inny, jeśli utrzymanie dochodów byłoby możliwe. Zmiennymi objaśniającymi były cechy społeczno-demograficzno-zawodowe: płeć, wiek i wykonywanie zabiegowej i niezabiegowej specjalności lekarskiej oraz subiektywna ocena aktualnej pracy zawodowej w aspekcie 10 atrybutów pracy lekarza. W analizie wykorzystano test χ2 Pearsona, współczynnik korelacji rang Spearmana oraz klasteryzację.
Wyniki. Zdecydowana większość lekarzy przyznała, że ich praca jest interesująca (95,9%), ale nerwowa (90,5%) i zagrażająca (77,8%). Nerwowość była istotnie częściej wskazywana przez młodych lekarzy. Chęć zmiany zawodu zadeklarowało 18,1% lekarzy. Chęć zmiany zawodu była najsilniej związana z opinią, że praca jest nieprzyjemna i odrażająca.
Wnioski. Więcej lekarzy zgłaszało wady swojej pracy niż jej zalety. Co piąty zadeklarował chęć zmiany zawodu. Chęć zmiany zawodu była silnie związana z całościowo negatywną oceną własnej pracy. Przypuszczalnie w wyniku pandemii COVID-19 coraz więcej lekarzy będzie negatywnie oceniać swoją pracę, co może skłaniać do zmiany zawodu. Sytuacja ta wymaga monitorowania wpływu COVID-19 na kadrę oraz podejmowania środków zaradczych.
Paracrine effects of adipose-derived stem cells in cutaneous wound healing in streptozotocin-induced diabetic rats
Authors: Luo Hua , Wang Yongjian , Su Yongwei , Liu Danping , Xiao Haijun , Wu Ming , Zhao Yong , Xue Feng 
Published: February 2022

Objective: The purpose of this study was to explore the paracrine effects of adipose-derived stem cells (ASCs) on cutaneous wound healing in diabetic rats Method: The ASCs were isolated and identified by immunofluorescent staining. The ASCs-conditioned medium (ASCs-CM) was harvested. Cell counting kit (CCK)-8 assay, scratch experiments, western blot and quantitative polymerase chain reaction (qPCR) were performed to observe the effects of ASCs-CM on fibroblasts. A full-thickness skin wound diabetic rat model was prepared, using 34 male, Sprague Dawley rats. ASCs-CM or negative-control medium (N-CM) was injected around the wound surface. The existing wound area was measured on days 4, 8, 12 and 16 after the postoperative day, and the wound tissues were collected for immunohistochemical staining and qPCR quantitative study. Results: In this experiment, the isolated cells were characterised as ASCs. The results of CCK-8 assay, cell scratch test, western blot and qPCR showed ASCs-CM could significantly promote the proliferation, migration and differentiation of fibroblasts. Simultaneously, the healing rate of full-thickness skin wounds in diabetic rats was significantly higher in the ASCs-CM group than the N-CM group on days 4, 8, 12 and 16. Immunohistochemical staining and qPCR results showed that the expression of vascular endothelial growth factor (VEGF, days 4 and 8), α-smooth muscle actin (SMA) (days 4 and 16), transforming growth factor (TGF)-β1 (days 4, 8 and 12) were higher in the ASCs-CM group than that of the N-CM group (p<0.05). Conclusion: This experiment demonstrated that ASCs-CM may accelerate wound healing in diabetic rats by promoting the secretion of TGF-β1, VEGF and the proliferation, migration and differentiation of fibroblasts.
Stage-differentiated ensemble modeling of DNA methylation landscapes uncovers salient biomarkers and prognostic signatures in colorectal cancer progression
Authors: Muthamilselvan Sangeetha , Raghavendran Abirami , Palaniappan Ashok 
Published: February 2022

Background
Aberrant DNA methylation acts epigenetically to skew the gene transcription rate up or down, contributing to cancer etiology. A gap in our understanding concerns the epigenomics of stagewise cancer progression. In this study, we have developed a comprehensive computational framework for the stage-differentiated modelling of DNA methylation landscapes in colorectal cancer (CRC).

Methods
The methylation β-matrix was derived from the public-domain TCGA data, converted into M-value matrix, annotated with AJCC stages, and analysed for stage-salient genes using an ensemble of approaches involving stage-differentiated modelling of methylation patterns and/or expression patterns. Differentially methylated genes (DMGs) were identified using a contrast against controls (adjusted p-value <0.001 and |log fold-change of M-value| >2), and then filtered using a series of all possible pairwise stage contrasts (p-value <0.05) to obtain stage-salient DMGs. These were then subjected to a consensus analysis, followed by matching with clinical data and performing Kaplan–Meier survival analysis to evaluate the impact of methylation patterns of consensus stage-salient biomarkers on disease prognosis.

Results
We found significant genome-wide changes in methylation patterns in cancer cases relative to controls agnostic of stage. The stage-differentiated models yielded the following consensus salient genes: one stage-I gene (FBN1), one stage-II gene (FOXG1), one stage-III gene (HCN1) and four stage-IV genes (NELL1, ZNF135, FAM123A, LAMA1). All the biomarkers were significantly hypermethylated in the promoter regions, indicating down-regulation of expression and implying a putative CpG island Methylator Phenotype (CIMP) manifestation. A prognostic signature consisting of FBN1 and FOXG1 survived all the analytical filters, and represents a novel early-stage epigenetic biomarker / target.

Conclusions
We have designed and executed a workflow for stage-differentiated epigenomic analysis of colorectal cancer progression, and identified several stage-salient diagnostic biomarkers, and an early-stage prognostic biomarker panel. The study has led to the discovery of an alternative CIMP-like signature in colorectal cancer, reinforcing the role of CIMP drivers in tumor pathophysiology.