bluuebunny/crossref_metadata_2025_split huggingface dataset:
The expression level of chicken telomerase reverse transcriptase in tumors induced by ALV-J is positively correlated with methylation and mutation of its promoter region
Authors: Xiang Yong , Chen Qinxi , Li Qingbo , Liang Canxin , Cao Weisheng 
Published: June 2022

AbstractAvian leukosis virus subgroup J (ALV-J) can cause neoplastic diseases in poultry and is still widely prevalent in China. Chicken telomerase reverse transcriptase (chTERT) is the core component of telomerase, which is closely related to the occurrence and development of tumors. Our previous studies showed that chTERT is overexpressed in ALV-J tumors, but the mechanism is still not completely clear. Therefore, this study aims to analyze the possible molecular mechanism of chTERT overexpression in ALV-J tumors from the perspective of DNA methylation and promoter mutation. Methylation sequencing of the chTERT amplicon showed that ALV-J replication promoted the methylation level of the chTERT promoter. And the methylation level of the chTERT promoter in ALV-J tumors was significantly higher than that in tumor-adjacent and normal tissues. Compared with the tumor-adjacent and normal tissues, the chTERT promoter in each ALV-J tumors tested had a mutation of −183 bp C > T, and 36.0% (9/25) of the tumors also had mutations of −184 bp T > C, −73 bp::GGCCC and −56 bp A > T in the chTERT promoter, which formed the binding sites for the transcription factors NFAT5, TFAP2A and ZEB1, respectively. The results of RT–qPCR and Western blotting showed that the occurrence of these mutations significantly increased the expression level of chTERT. In conclusion, this study demonstrated that the high expression of chTERT in ALV-J tumors is positively correlated with the level of hypermethylation and mutation in its promoter, which provides a new perspective for further research on the molecular mechanism of chTERT in ALV-J tumorigenesis.
The rooting ability of in vitro shoot cultures established from a UK collection of the common ash (Fraxinus excelsior L.) and their ex vitro survival
Authors: Fenning Trevor , O’Donnell Margaret , Preedy Katharine , Bézanger Aurélia , Kenyon David , Lopez Gustavo 
Published: June 2022

AbstractKey messageShoot cultures from a population of British ash trees (Fraxinus excelsiorL.) were assessed for their ability to form rootsin vitroand to grow in nursery conditions. Most of the plants survived whether they had formed roots or not, but this was affected by a systemic contaminant. This information will be valuable for work aimed at overcoming ash die-back disease.ContextAsh die-back disease is killing Europe’s ash trees, with much breeding and experimental work is underway to help overcome it, including this.AimsThis work aimed to develop and test a set of standard tissue culture methods for propagating material from selected ash trees, and then to produce rooted clonal plants which could be transferred to nursery conditions.MethodsSeed material from a range of British and Irish ash provenances were used for this work, with the shoot cultures being maintained on DKW based media, with 3 ppm BAP being added to induce shoot proliferation, with the shoots so generated being exposed to 3 ppm IBA to induce root formation in vitro.ResultsOne hundred thirty-six shoot cultures were successfully established from 13 mother trees. Most were generated from hypocotyl pieces excised from sterile germinating ash seeds on DKW medium plus 3 ppm BAP. Another 24 cultures were lost to a bacterial contaminant, which was provisionally identified as the plant symbiontBacillus megateriumor a close relative. Overall, 41.5% of uncontaminated plants and 11.6% of contaminated plants produced roots in vitro, after exposure to DKW medium with 3 ppm IBA, followed by hormone-free medium, with 92.6% of the uncontaminated plants surviving the transfer to the nursery whether they were rooted or not, as opposed to 62.1% of the contaminated plants.ConclusionsThis methodology can be used to produce large numbers of clonal ash plants on demand from a wide cross-section of the UK’s and Ireland’s ash breeding population, and so can be used to help produce the ash plants needed to combat ash die-back disease, although contamination issues are likely to remain an issue.
Solar and lunar daily geomagnetic variations and their equivalent current systems observed by Swarm
Authors: Yamazaki Yosuke 
Published: June 2022

AbstractThis paper describes solar and lunar daily variations of the geomagnetic field over low- and mid-latitude regions, using vector magnetometer data from Swarm satellites at altitudes of $$\sim$$
∼
500 km during the solar minimum years of 2017–2020. The average solar variation of the geomagnetic field is within the range of ±14 nT, while the lunar variation is within ±2 nT. The latter is comparable to the ocean tidal field. A spherical harmonic analysis is performed on the solar and lunar variations to evaluate their internal and external equivalent current systems. The results show that both the solar and lunar variations are mainly of internal origin, which can be attributed to combined effects of ionospheric dynamo currents and induced underground currents. Global patterns of the internal solar and lunar current systems are consistent with the corresponding external current systems previously reported based on ground observations. The Swarm external currents are mainly in the meridional direction, and are likely associated with interhemispheric field-aligned currents. Both the internal and external current systems depend on the season and longitude.
Graphical Abstract
Adherence to Mediterranean diet associated with health-related quality of life in children and adolescents: a systematic review
Authors: Romero-Robles Milton A. , Ccami-Bernal Fabricio , Ortiz-Benique Zhamanda N. , Pinto-Ruiz Diego F. , Benites-Zapata Vicente A. , Casas Patiño Donovan 
Published: June 2022

Abstract
Background
Health-related quality of life (HRQoL) has become a significant outcome in assessing interventions in the pediatric population and could be influenced by diet patterns. The Mediterranean diet (MD) pattern has been related to multiple positive health outcomes, including decreased cardiovascular risk and better mental health. We aimed to evaluate the association between MD adherence and HRQoL in children and adolescents.

Methods
The literature search was conducted in PubMed, Cochrane Library, Scopus, Web of Science, Embase, and Ovid-MEDLINE databases from inception to May 2022. Two researchers independently checked titles and abstracts, evaluated full-text studies, extracted data, and appraised the risk of bias using the Newcastle–Ottawa Scale (NOS).

Results
Eleven studies (1 longitudinal and 10 cross-sectional), totaling 6,796 subjects, were included. Ten studies assessed MD adherence with KIDMED index, and one assessed MD adherence with Krece Plus test, while all included studies assessed HRQoL with a KIDSCREEN test. All studies analyzed the association between MD adherence and HRQoL with linear regression, and eight used adjusted models. Five studies found a significant positive association of MD adherence with HRQoL, with β-values ranging from 0.13 to 0.26. Two found a nonsignificant positive relationship, while one found a negative association. According to the NOS criteria, the risk of bias assessment showed four studies with a low risk of bias and seven with a high risk of bias.

Conclusion
Our findings suggest a positive correlation of MD adherence with HRQoL in children and adolescents. However, future research is needed to strengthen the evidence of this relationship.

Trial registration
CRD42021236188 (PROSPERO)
Health-related quality of life and social determinants of health following COVID-19 infection in a predominantly Latino population
Authors: Case Kathleen R. , Wang Chen-Pin , Hosek Meredith G. , Lill Sarah F. , Howell Alexandra B. , Taylor Barbara S. , Bridges James , MacCarthy Daniel J. , Winkler Paula , Tsevat Joel 
Published: June 2022

Abstract
Background
As the COVID-19 pandemic evolves, more information is needed on its long-term impacts on health-related quality of life (HRQoL) and social determinants of health (SDoH). The aim of the study was to assess HRQoL and SDoH among a predominantly Latino population of COVID-19 survivors and to compare effects in Latinos versus non-Latinos.

Methods
This cross-sectional study consisted of a survey (in English and Spanish) of COVID-19 survivors from December 2020 to July 2021. The study assessed sociodemographic data, clinical characteristics, and SDoH, consisting of 10 COVID-19—related concerns. The PROMIS-29 + 2 (PROPr) measure, which captures 8 HRQoL domains and a preference-based health utility, was used to assess HRQoL. Bivariate analyses included chi-square tests and t-tests. Generalized linear models were conducted for multivariable analyses.

Results
Of 230 respondents (6.3% response rate), the mean [SD] age was 43.1 [14.3] years; 83.0% were Latino; the mean [SD] time since diagnosis was 8.1 [3.2] months; and 12.6% had a history of hospitalization with COVID-19. HRQoL scores were slightly worse than population norms on all domains, especially anxiety; the mean [SD] PROPr health utility was 0.36 [0.25]. Domain scores were similar by ethnicity except for cognitive function—abilities, where scores were lower in Latinos. Multivariable analyses revealed that: (1) financial concerns were associated with worse health utility, as well as worse scores on all 8 PROMIS domains; (2) interpersonal conflict was associated with worse health utility and worse scores on 6 of the 8 PROMIS domains (anxiety, depression, fatigue, sleep disturbance, social function, and pain interference); and (3) Latino ethnicity was only associated with 1 PROMIS domain (cognitive function—abilities) after controlling for covariates.

Conclusion
COVID-19 infection is associated with HRQoL decrements long after the acute infection, and financial concerns and interpersonal conflict are particularly associated with worse HRQoL.
Prevalence and determinants of anxiety in patients with epilepsy during COVID-19 pandemic
Authors: Sehlo Mohammad Gamal , Mohamed Wafaa Samir , Youssef Usama Mahmoud , Lotfi Shrouk Esam , El-deen Ghada Mohamed Salah 
Published: June 2022

Abstract
Background
Epilepsy is one of the most frequent and serious brain disorders. The nature of the disorder and the unpredictability of seizures usually puts patients in a state of apprehension and anticipation, which creates a continuous condition of anxiety. COVID-19 pandemic has created a state of generalized anxiety all over the world. It is expected that patients with epilepsy (PWE) will suffer from more anxiety during the pandemic. This cross-sectional study was applied on 290 PWE. Data were collected by personal interview with each patient using GAD-7 scale for diagnosing anxiety and assessing its severity. We aimed to assess the prevalence of anxiety and to assess its risk factors in PWE during COVID-19 pandemic.

Results
We found that 52.4% of PWE suffered from anxiety. Not working, low financial status, fear of infection and death by COVID-19, fear of job loss, had job changes during pandemic, increased seizures rate during pandemic, increased ER visits, and lack of drug adherence during the pandemic, are significantly associated with increased risk of anxiety.

Conclusions
COVID-19 pandemic has a serious effect on the psychological and the physical wellbeing of PWE. There was an increased rate of anxiety during COVID-19 pandemic in PWE with its subsequent burden on those patients. So, these patients are in a high need of care and support during the pandemic.
Impact of exercise training on myocardial contractile functions assessed by cardiac magnetic resonance in post-myocardial infarction patients
Authors: Elshazly Ahmed , Hasan Rana Lateef , Saleh Mohamed Ayman , Ibrahim Ahmed Samir , Khorshid Hazem 
Published: June 2022

Abstract
Background
Improvement of functional capacity and mortality reduction in post-MI patients were found to be associated with regular exercise training. The cardiac magnetic resonance (CMR) is considered the most accurate non-invasive modality in quantitative assessment of left ventricular (LV) volumes and systolic functions. Our main objective was to investigate the impact of exercise training on LV systolic functions in patients post anterior MI using CMR. 32 patients on recommended medical treatment 4 week after having a successful primary PCI for an anterior MI were recruited, between May 2018 and May 2019. They were divided into two groups, training group (TG): 16 assigned to a 12 week exercise training program and control group (CG): 16 who received medical treatment without participating in the exercise training program. Treadmill exercise using modified Bruce protocol was done to TG before and after the training program in order to record the resting and maximum HR, metabolic equivalent (MET), and calculate HR reserve. CMR was performed for all patients 4 weeks after PCI and was repeated after completion of the study period to calculate ejection fraction (EF), left ventricular end-systolic volume (LVESV), left ventricular end-diastolic volume (LVEDV), and wall motion score index (WMSI).

Results
100% were males. 6 patients from CG dropped during follow-up, no statistically significant difference between the two groups regarding age, BMI, smoking status, hypertension, diabetes mellitus and dyslipidemia. Using the CMR, the TG showed significant improvement in EF (36.6 ± 14.2% to 43.1 ± 12.4%; P < 0.001) and WMSI (2.03 ± 0.57 to 1.7 ± 0.49; P < 0.001), without statistically significant change in LV volumes. Regarding CG no significant changes in EF, WMSI, LV volumes were found. There was significant improvement in EF and WMSI change before and after study in TG vs. CG [6.5 (2.3–9.0) vs. − 2.0 (− 6.8 to 1.3), P value < 0.001] and [− 0.3 (− 0.5 to 0.1) vs. 0.1 (− 0.1 to − 0.5), P value 0.001] respectively.

Conclusions
12 weeks of exercise training program in post-MI patients have a favorable impact on LV global and regional systolic functions without adversely affecting LV remodeling (as assessed by CMR).
Laminar shear stress alleviates monocyte adhesion and atherosclerosis development via miR-29b-3p/CX3CL1 axis regulation
Authors: Pu Luya , Meng Qingyu , Li Shuai , Wang Yaru , Sun Banghao , Liu Bin , Li Fan 
Published: June 2022

ABSTRACTLaminar shear stress (Lss) is an important anti-atherosclerosis (anti-AS) factor, but its mechanism network is not clear. Therefore, this study aimed to identify how Lss acts against AS formation from a new perspective. In this study, we analyzed high-throughput sequencing data from static and Lss-treated human aortic and human umbilical vein endothelial cells (HAECs and HUVECs, respectively) and found that the expression of CX3CL1, which is a target gene closely related to AS development, was lower in the Lss group. Lss alleviated the inflammatory response in TNF-α (also known as TNF)-activated HAECs by regulating the miR-29b-3p/CX3CL1 axis, and this was achieved by blocking nuclear factor (NF)-κB signaling. In complementary in vivo experiments, a high-fat diet (HFD) induced inflammatory infiltration and plaque formation in the aorta, both of which were significantly reduced after injection of agomir-miRNA-29b-3p via the tail vein into HFD-fed ApoE−/− mice. In conclusion, this study reveals that the Lss-sensitive miR-29b-3p/CX3CL1 axis is an important regulatory target that affects vascular endothelial inflammation and AS development. Our study provides new insights into the prevention and treatment of AS.
Excellent Enantioselective Organocatalytic One-Pot Desymmetrization of meso-1,2-Diols through Asymmetric Acylation and Silylation by a Chiral 1,2-Diamine Derived from (S)-Proline
Authors: Nakayama Hirofumi , Urai Hitomi , Oriyama Takeshi 
Published: June 2022

Abstract
The one-pot desymmetrization of meso-1,2-diols was successfully performed via their benzoylation, and subsequent silylation to give the corresponding siloxy esters in excellent enantioselectivities. The reduction of these siloxy esters afforded the corresponding β-siloxy alcohols without any loss in enantioselectivity.
Detecting Sleep and Nonwear in 24-h Wrist Accelerometer Data from the National Health and Nutrition Examination Survey
Authors: THAPA-CHHETRY BINOD , ARGUELLO DIEGO JOSE , JOHN DINESH , INTILLE STEPHEN 
Published: June 2022

ABSTRACT

Introduction
Estimating physical activity, sedentary behavior, and sleep from wrist-worn accelerometer data requires reliable detection of sensor nonwear and sensor wear during both sleep and wake.

Purpose
This study aimed to develop an algorithm that simultaneously identifies sensor wake-wear, sleep-wear, and nonwear in 24-h wrist accelerometer data collected with or without filtering.

Methods
Using sensor data labeled with polysomnography (n = 21) and directly observed wake-wear data (n = 31) from healthy adults, and nonwear data from sensors left at various locations in a home (n = 20), we developed an algorithm to detect nonwear, sleep-wear, and wake-wear for “idle sleep mode” (ISM) filtered data collected in the 2011–2014 National Health and Nutrition Examination Survey. The algorithm was then extended to process original raw data collected from devices without ISM filtering. Both algorithms were further validated using a polysomnography-based sleep and wake-wear data set (n = 22) and diary-based wake-wear and nonwear labels from healthy adults (n = 23). Classification performance (F1 scores) was compared with four alternative approaches.

Results
The F1 score of the ISM-based algorithm on the training data set using leave-one-subject-out cross-validation was 0.95 ± 0.13. Validation on the two independent data sets yielded F1 scores of 0.84 ± 0.60 for the data set with sleep-wear and wake-wear and 0.94 ± 0.04 for the data set with wake-wear and nonwear. The F1 score when using original, raw data was 0.96 ± 0.08 for the training data sets and 0.86 ± 0.18 and 0.97 ± 0.04 for the two independent validation data sets. The algorithm performed comparably or better than the alternative approaches on the data sets.

Conclusions
A novel machine-learning algorithm was designed to recognize wake-wear, sleep-wear, and nonwear in 24-h wrist-worn accelerometer data that are applicable for ISM-filtered data or original raw data.
Case Article—Analysis of Call Center Data at Patelco Credit Union
Authors: Agrawal Narendra 
Published: June 2022

This case focuses on operational challenges faced by customer service centers, or call centers. The specific context for these cases is Patelco, which is a California based credit union. Patelco is facing a rising number of complaints about customer service, specifically, about the long delay customers had to face when they called one of Patelco‘s four call centers. The purpose of this case is to expose students to the statistical analysis of some of the raw data, obtained from such an environment, to support the investigation of call center performance. Important managerial insights can be drawn by summarizing the data graphically as well as quantitatively. In particular, the data can be used to show students how to perform a number of useful hypothesis tests, which are often needed to answer important questions that arise when assessing the performance of any system. The outcomes from these analyses can provide important managerial insights. In the operations management literature, and in practice, a number of modeling assumptions are made about the distribution of data to facilitate quantitative analysis. However, these assumptions are often not statistically validated. This case study gives students an opportunity to test such assumptions. This case can be used in graduate and undergraduate classes in Operations Management, Supply Chain Management and Service Operations to review statistical concepts related to the topic of queuing analysis, or in a Statistics class to illustrate data analysis and statistical tests in a real-world context. Supplemental Material: Supplemental material is available at https://doi.org/10.1287/ited.2022.0272ca and the Teaching Notes and Excel spreadsheet of solutions are available at https://www.informs.org/Publications/Subscribe/Access-Restricted-Materials .
The current and future role of nurses within enhanced recovery after surgery pathways
Authors: Wainwright Thomas W , Jakobsen Dorthe Hjort , Kehlet Henrik 
Published: June 2022

Background: Enhanced recovery after surgery (ERAS) pathways have been proven to expedite recovery after many procedures and reduce lengths of stay in hospital and surgical complications. However, improvements are still needed, especially in postoperative ERAS components delivered by nurses such as early mobilisation and oral feeding. This article summarises the current and possible future role of nurses within ERAS, and recommends areas for future research. Discussion: Nurses are the professionals who spend the most time with patients throughout the perioperative pathway and are known to play a vital role in delivering many components of an ERAS pathway. They frequently co-ordinate care across disciplines and ensure continuity of care. However, there is a paucity of ERAS research specific to nurses compared to other professional groups. Continual training on ERAS will be required to ensure nurses are highly educated and for the best possible ERAS implementation. In certain types of surgery, nurses may fulfil extended roles in the postoperative period, such as taking over responsibility and leadership for co-ordinating pain management, mobilisation and discharge. However, this requires a well-defined care programme, a clear definition of nursing responsibilities from surgeons, agreed discharge criteria and highly qualified nurses, along with the collection and analysis of data to test safety and efficacy. Conclusion: Increasing nurse involvement in ERAS research is vital to drive improvements in care and to develop nursing roles. Nurses should have a major role in the preoperative clinic, the early postoperative phase and the follow-up post-discharge period, where the benefits of ERAS need to be further documented.
Effective all-fiber pump recycler for kilowatt fiber lasers
Authors: Lin Weixuan , Desjardins-Carrière Maxime , Iezzi Victor Lambin , Vincelette André , Bussières-Hersir Marie-Hélène , Rochette Martin 
Published: June 2022

We report an effective pump recycler for industrial kilowatt fiber lasers. The pump recycler is a

(
6
+
1
)
×
1

tapered fiber bundle, with signal ports of Ge-doped fiber (GDF) with core/cladding diameters of 20/400 µm and pump fiber ports (PFPs) with core/cladding diameters of 135/155 µm. By splicing PFPs in pairs, 77.9% of the residual pump light reaching the pump recycler is sent back to the cladding of the GDF. The insertion of a pump recycler increases the power conversion efficiency (PCE) of a fiber laser using an Yb-doped fiber (YDF) from 61.0% to 70.5%, with a maximum output power of 2.78 kW. The laser with a 20 m long YDF and pump recycler compares well to another laser using a 40 m long YDF without pump recycler. In both cases, the PCE is comparable but the laser with a 20 m long YDF and pump recycler benefits from reduced stimulated Raman scattering (SRS), thus enabling an 80% increase in Raman threshold. By giving access to short YDF length, the tapered fiber bundle represents an effective pump recycler since it enables reducing SRS while keeping a large PCE.