bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incentives and constraints: a configurational account of European involvement in the anti-Daesh coalition
Authors: Mello Patrick A. 
Published: February 2022

AbstractIn 2014, the USA initiated the formation of a multilateral military operation against Daesh in Syria and Iraq. Eventually, more than 70 states joined the anti-Daesh coalition. However, contributions to the military effort have been characterized by great variance, especially among EU member states. While some states took leading roles in the airstrikes, others provided training for Iraqi and Kurdish forces, and still others did not get involved beyond voicing their support for the policy. Against this backdrop, this article makes a two-fold contribution to the literature on military coalitions and security policy. Empirically, the article provides a mapping of the then 28 EU member states’ military engagement in the fight against Daesh in Syria and Iraq. Analytically, fuzzy-set Qualitative Comparative Analysis (fsQCA) is applied to account for the observed pattern of military involvement, using an integrative framework that combines international and domestic factors. The results demonstrate that multiple paths led towards EU military involvement in the anti-Daesh coalition. At the same time, international-level incentives, such as external threat and/or alliance value feature prominently in all three identified paths. The analysis further underscores the value of a configurational perspective, because neither an external threat nor alliance value are sufficient on their own to bring about the outcome. Across the set-theoretic configurations, these conditions either combine with other ‘push’ factors or with the absence of constraints against military involvement. In line with the latter, the article highlights the policy relevance of institutional constraints, especially legislative veto rights, since most of those countries that were involved in the airstrikes of the anti-Daesh coalition did not have formal parliamentary involvement on matters of military deployment policy.
Severe Lake Heatwaves Attributable to Human‐Induced Global Warming
Authors: Woolway R. Iestyn , Albergel Clément , Frölicher Thomas L. , Perroud Marjorie 
Published: February 2022

AbstractMuch of the focus of global warming impacts on lakes have focused on changes in mean temperature. However, lakes are also highly vulnerable to thermal extremes. Such extremes occur, by definition, during lake heatwaves. Heatwaves in lakes have occurred globally in recent decades and have had severe negative impacts. However, unlike their atmospheric counterparts, it is currently unknown to what extent lake heatwaves are altered by human‐induced climate change. Here, we estimate the human contribution to lake heatwaves, specifically focusing on the most severe events. We demonstrate that the occurrence probabilities of severe lake heatwaves increase substantially due to human influence. Our analysis suggests that 94% of severe heatwaves observed during the satellite data‐taking period have an anthropogenic contribution. Globally, we suggest that severe heatwaves are 3 and 25‐ times more likely in a 1.5°C and 3.5°C warmer world, respectively, compared to a world without anthropogenic influence.
The Balance Between Heterogeneous and Homogeneous Nucleation of Ice Clouds Using CAM5/CARMA
Authors: Maloney Christopher , Toon Brian , Bardeen Charles , Yu Pengfei , Froyd Karl , Kay Jennifer , Woods Sarah 
Published: February 2022

AbstractWe present a modification to the Community Aerosol and Radiation model for Atmospheres (CARMA) sectional ice microphysical model where we have added interactive nucleation of sulfates and heterogeneous nucleation onto dust in order to create a more comprehensive representation of ice nucleation within the CARMA sectional ice model. The Yu et al. (2019, https://doi.org/10.1029/2018gl080544) convective wet removal fix has also been added in order to correctly transport aerosol within the Community Atmosphere Model version 5 (CAM5) and the 3‐mode Modal Aerosols Model (MAM3). In CARMA, the balance of homogeneous and heterogeneous nucleation is controlled by the presence of temperatures below 240 K, supersaturation, and the availability of heterogeneous nuclei. Due to a paucity of dust at altitudes above about 7 km, where temperatures over most of the Earth fall below 240 K, cirrus clouds above 7 km nucleate primarily via homogeneous nucleation on aqueous sulfate aerosols in our simulations. Over mid‐latitudes of the Northern Hemisphere, dust is more common above 7 km during spring through fall, and both heterogeneous nucleation and homogenous freezing occur in our model. Below 7 km heterogeneous nucleation dominates in situ formation of ice. Furthermore, we find an improvement of the representation of in‐cloud ice within mixed phase clouds in CAM5/CARMA when compared to simulations with only homogeneous ice nucleation. Other modes of nucleation such as contact nucleation of liquid cloud droplets or liquid cloud droplet freezing on immersion nuclei, were not directly compared with classical depositional heterogeneous nucleation in this study.
Collocated Measurements of Light‐Absorbing Organic Carbon in PM2.5: Observation Uncertainty and Organic Tracer‐Based Source Apportionment
Authors: Xie Mingjie , Peng Xiang , Shang Yue , Yang Li , Zhang Yuanyuan , Wang Yuhang , Liao Hong 
Published: February 2022

AbstractIn this study, collocated filter samples of particulate matter with aerodynamic diameter less than 2.5 μm (PM2.5) from northern Nanjing were extracted using water and methanol, followed by analysis of light absorption. A backup quartz filter was used to correct sampling artifacts caused by adsorption of gaseous organics. The collocated precision of light‐absorbing properties of water‐soluble organic carbon (WSOC) and methanol‐extractable organic carbon (MEOC) were parameterized using correlation coefficient (r), coefficient of divergence (COD), and average relative percent difference (ARPD, %). In general, the light absorption of WSOC and MEOC showed good agreement (r > 0.80, COD < 0.20) between collocated samples. Performing artifact correction is necessary and will increase the heterogeneity between collocated measurements. The duplicate‐derived ARPD values of MEOC absorption were more than 60% higher than those of WSOC absorption. Then, it would be inappropriate to assume a uniform uncertainty fraction (e.g., ∼10%) for WSOC and MEOC absorption in future studies on their climate effects and source apportionment. To apportion artifact‐corrected absorption of aerosol extracts to specific emission sources or formation pathways, positive matrix factorization was performed by using concentration data of selected bulk species and organic molecular markers. Among the nine identified factor/sources, the biomass burning factor had the highest average contributions to the absorption of both WSOC (31.6%) and MEOC (48.0%), followed by dust resuspension and coal combustion factors. Unlike combustion‐related primary emissions, the factors containing influences from atmospheric processing (e.g., secondary nitrate) contributed more fractions of WSOC absorption than MEOC absorption.
Stratospheric Influence on the Development of the 2018 Late Winter European Cold Air Outbreak
Authors: Huang Jinlong , Hitchcock Peter , Tian Wenshou , Sillin Jack 
Published: February 2022

AbstractUsing ensemble forecasts from the Global Ensemble Forecast System (GEFS) and controlled integrations with the Weather Research and Forecasting (WRF) model, we explore the stratospheric influence on the development of the 2018 European severe cold air outbreak (CAO). The 2018 CAO set in on 22 February, and its evolution was closely related to a growing tropospheric blocking ridge over the North Atlantic in late February that brought cold air mass into Europe. The event was also preceded by a split of the stratospheric polar vortex on 12 February, after which one child vortex persisted over northeastern Canada. GEFS forecasts initialized 1 week prior to this CAO substantially underestimated its severity, exhibiting large spread correlated with the strength and location of the Atlantic ridge, while forecasts initialized closer to the event captured its severity with higher fidelity. Based on a set of nudged simulations, we show that capturing the evolution of the stratospheric child vortex over northeastern Canada, particularly in the lowermost stratosphere, is of great importance for predicting this CAO, since it substantially affects the strength and location of the Atlantic ridge that in turn determines the evolution and severity of this CAO. When the stratosphere is nudged toward the observed evolution, the northward flow between the child vortex and the Atlantic ridge advects more low potential vorticity air into the Atlantic ridge, and therefore amplifies it. Further experiments demonstrate that the impact of the stratosphere on the development of the ridge is particularly strong over the North Atlantic.
Quantitative electrophysiological assessments as predictive markers of lower limb motor recovery after spinal cord injury: a pilot study with an adaptive trial design
Authors: Huang Yin Nan , Meftah El-Mehdi , Pion Charlotte H. , Mac-Thiong Jean-Marc , Cohen-Adad Julien , Barthélemy Dorothy 
Published: February 2022

Abstract
Study design
Observational, cohort study.

Objectives
(1) Determine the feasibility and relevance of assessing corticospinal, sensory, and spinal pathways early after traumatic spinal cord injury (SCI) in a rehabilitation setting. (2) Validate whether electrophysiological and magnetic resonance imaging (MRI) measures taken early after SCI could identify preserved neural pathways, which could then guide therapy.

Setting
Intensive functional rehabilitation hospital (IFR).

Methods
Five individuals with traumatic SCI and eight controls were recruited. The lower extremity motor score (LEMS), electrical perceptual threshold (EPT) at the S2 dermatome, soleus (SOL) H-reflex, and motor evoked potentials (MEPs) in the tibialis anterior (TA) muscle were assessed during the stay in IFR and in the chronic stage (>6 months post-SCI). Control participants were only assessed once. Feasibility criteria included the absence of adverse events, adequate experimental session duration, and complete dataset gathering. The relationship between electrophysiological data collected in IFR and LEMS in the chronic phase was studied. The admission MRI was used to calculate the maximal spinal cord compression (MSCC).

Results
No adverse events occurred, but a complete dataset could not be collected for all subjects due to set-up configuration limitations and time constraints. EPT measured at IFR correlated with LEMS in the chronic phases (r = −0.67), whereas SOL H/M ratio, H latency, MEPs and MSCC did not.

Conclusions
Adjustments are necessary to implement electrophysiological assessments in an IFR setting. Combining MRI and electrophysiological measures may lead to better assessment of neuronal deficits early after SCI.
PCLAF promotes neuroblastoma G1/S cell cycle progression via the E2F1/PTTG1 axis
Authors: Liu Xiaowei , Cai Yuanxia , Cheng Cheng , Gu Yaoyao , Hu Xiaoxiao , Chen Kai , Wu Yeming , Wu Zhixiang 
Published: February 2022

AbstractPCLAF (PCNA clamp-associated factor), also known as PAF15/ KIAA0101, is overexpressed in most human cancers and is a predominant regulator of tumor progression. However, its biological function in neuroblastoma remains unclear. PCLAF is extremely overexpressed in neuroblastoma and is associated with poor prognosis. Through the analysis of various data sets, we found that the high expression of PCLAF is positively correlated with increased stage and high risk of neuroblastoma. Most importantly, knocking down PCLAF could restrict the proliferation of neuroblastoma cells in vitro and in vitro. By analyzing RNA-seq data, we found that the enrichment of cell cycle-related pathway genes was most significant among the differentially expressed downregulated genes after reducing the expression of PCLAF. In addition, PCLAF accelerated the G1/S transition of the neuroblastoma cell cycle by activating the E2F1/PTTG1 signaling pathway. In this study, we reveal the mechanism by which PCLAF facilitates cell cycle progression and recommend that the PCLAF/E2F1/PTTG1 axis is a therapeutic target in neuroblastoma.
c-Myb protects cochlear hair cells from cisplatin-induced damage via the PI3K/Akt signaling pathway
Authors: Bu Chuan , Xu Lei , Han Yuechen , Wang Man , Wang Xue , Liu Wenwen , Chai Renjie , Wang Haibo 
Published: February 2022

AbstractThe transcription factor c-Myb is vital for cell survival, proliferation, differentiation, and apoptosis. We have previously reported that c-Myb knockdown exacerbates neomycin-induced damage to cochlea cells. However, the function and regulation of c-Myb in the mammalian inner ear remains unclear. Here, we first found that the expression of c-Myb in cochlear HCs was downregulated after cisplatin damage in vivo. Next, to investigate the role of c-Myb in HCs treated with cisplatin, the recombinant virus AAV-ie-CAG-Myb-HA (AAV-c-Myb) that overexpresses c-Myb was constructed and transfected into HCs. The protein expression of c-Myb was effectively up-regulated in cultured cochlear HCs after the virus transfection, which increased cochlear HC viability, decreased HC apoptosis and reduced intracellular reactive oxygen species (ROS) levels after cisplatin injury in vitro. The overexpression of c-Myb in HCs after AAV-c-Myb transfection in vivo also promoted HC survival, improved the hearing function of mice and reduced HC apoptosis after cisplatin injury. Furthermore, c-Myb-HC conditional knockout mice (Prestin; c-Myb-cKO) in which c-Myb expression is downregulated only in cochlear OHCs were generated and the cisplatin-induced HCs loss, apoptosis and hearing deficit were all exacerbated in Prestin; c-Myb-cKO mice treated with cisplatin in vivo. Finally, mechanistic studies showed that upregulation of the PI3K/Akt signaling pathway by c-Myb contributed to the increased HC survival after cisplatin exposure in vitro. The findings from this work suggest that c-Myb might serve as a new target for the prevention of cisplatin-induced HC damage and hearing loss.
METTL3 suppresses neuropathic pain via modulating N6-methyladenosine-dependent primary miR-150 processing
Authors: Zhang Le , Zhao Xin , Wang Jing , Jin Yanwu , Gong Moxuan , Ye Yuyang , Li Peilong 
Published: February 2022

AbstractMethyltransferase-like 3 (METTL3)-modulated N6-methyladenosine (m6A) was recently identified as an important epigenetic regulation type during RNA processing and contributes to multiple pathological processes. Neuropathic pain (NP) is induced by a lesion of the somatosensory nervous system, and the detailed pathways by which METTL3/m6A regulated to modulate gene dysregulation and enable NP have remained unclear. Therefore, this study investigated the function of METTL3-mediated m6A methylation on miRNA maturation, and investigated how this regulation contributes to NP progression. A rat model characterized with typical NP was established by a spared nerve-injury (SNI) method. By analyzing the expression levels of METTL3 and m6A methylation, we found that METTL3, along with m6A methylation, was dramatically downregulated in NP rats in contrast to the sham ones. Functionally, enhanced METTL3 promoted the m6A methylation in total RNAs and inhibited NP progression, whereas silencing METTL3 suppressed m6A methylation and increased NP severity. Mechanistically, METTL3 accelerated miR-150 maturation via mediating m6A methylation of primiR-150 at locus 498, cooperating with the “m6A reader” YTHDF2. Meanwhile, miR-150 could directly target brain-derived neurotrophic factor (BDNF) mRNA, and the METTL3/miR-150/BDNF regulatory pathway was finally established. Clinically, we proved that serum METTL3 mRNA was also downregulated in Shingles patients with NP, suggesting its diagnostic potential. In conclusion, we demonstrated an essential function of METTL3-regulated N6-methyladenosine during NP progression via modulating primiR-150 maturation. Serum METTL3 could effectively differentiate NP patients from healthy people, and is useful for dynamic monitoring of diseases after treatment. Therefore, the METTL3/miR-150/BDNF pathway may be a promising therapeutic target for NP patients.
Liquid crystalline 2D borophene oxide for inorganic optical devices
Authors: Kambe Tetsuya , Imaoka Shotaro , Shimizu Misa , Hosono Reina , Yan Dongwan , Taya Hinayo , Katakura Masahiro , Nakamura Hirona , Kubo Shoichi , Shishido Atsushi , Yamamoto Kimihisa 
Published: February 2022

AbstractBorophene has been recently proposed as a next-generation two-dimensional material with promising electronic and optical properties. However, its instability has thus far limited its large-scale applications. Here, we investigate a liquid-state borophene analogue with an ordered layer structure derived from two-dimensional borophene oxide. The material structure, phase transition features and basic properties are revealed by using X-ray analysis, optical and electron microscopy, and thermal characterization. The obtained liquid crystal exhibits high thermal stability at temperatures up to 350 °C and an optical switching behaviour driven by a low voltage of 1 V.
Single-cell profiling of human subventricular zone progenitors identifies SFRP1 as a target to re-activate progenitors
Authors: Donega Vanessa , van der Geest Astrid T. , Sluijs Jacqueline A. , van Dijk Roland E. , Wang Chi Chiu , Basak Onur , Pasterkamp R. Jeroen , Hol Elly M. 
Published: February 2022

AbstractFollowing the decline of neurogenesis at birth, progenitors of the subventricular zone (SVZ) remain mostly in a quiescent state in the adult human brain. The mechanisms that regulate this quiescent state are still unclear. Here, we isolate CD271+ progenitors from the aged human SVZ for single-cell RNA sequencing analysis. Our transcriptome data reveal the identity of progenitors of the aged human SVZ as late oligodendrocyte progenitor cells. We identify the Wnt pathway antagonist SFRP1 as a possible signal that promotes quiescence of progenitors from the aged human SVZ. Administration of WAY-316606, a small molecule that inhibits SFRP1 function, stimulates activation of neural stem cells both in vitro and in vivo under homeostatic conditions. Our data unravel a possible mechanism through which progenitors of the adult human SVZ are maintained in a quiescent state and a potential target for stimulating progenitors to re-activate.
Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo
Authors: Uenaka Maki , Yamashita Erika , Kikuta Junichi , Morimoto Akito , Ao Tomoka , Mizuno Hiroki , Furuya Masayuki , Hasegawa Tetsuo , Tsukazaki Hiroyuki , Sudo Takao , Nishikawa Keizo , Okuzaki Daisuke , Motooka Daisuke , Kosaka Nobuyoshi , Sugihara Fuminori , Boettger Thomas , Braun Thomas , Ochiya Takahiro , Ishii Masaru 
Published: February 2022

AbstractBone metabolism is regulated by the cooperative activity between bone-forming osteoblasts and bone-resorbing osteoclasts. However, the mechanisms mediating the switch between the osteoblastic and osteoclastic phases have not been fully elucidated. Here, we identify a specific subset of mature osteoblast-derived extracellular vesicles that inhibit bone formation and enhance osteoclastogenesis. Intravital imaging reveals that mature osteoblasts secrete and capture extracellular vesicles, referred to as small osteoblast vesicles (SOVs). Co-culture experiments demonstrate that SOVs suppress osteoblast differentiation and enhance the expression of receptor activator of NF-κB ligand, thereby inducing osteoclast differentiation. We also elucidate that the SOV-enriched microRNA miR-143 inhibits Runt-related transcription factor 2, a master regulator of osteoblastogenesis, by targeting the mRNA expression of its dimerization partner, core-binding factor β. In summary, we identify SOVs as a mode of cell-to-cell communication, controlling the dynamic transition from bone-forming to bone-resorbing phases in vivo.
Slab control on the mega-sized North Pacific ultra-low velocity zone
Authors: Li Jiewen , Sun Daoyuan , Bower Dan J. 
Published: February 2022

AbstractUltra-low velocity zones (ULVZs) are localized small-scale patches with extreme physical properties at the core-mantle boundary that often gather at the margins of Large Low Velocity Provinces (LLVPs). Recent studies have discovered several mega-sized ULVZs with a lateral dimension of ~900 km. However, the detailed structures and physical properties of these ULVZs and their relationship to LLVP edges are not well constrained and their formation mechanisms are poorly understood. Here, we break the degeneracy between the size and velocity perturbation of a ULVZ using two orthogonal seismic ray paths, and thereby discover a mega-sized ULVZ at the northern edge of the Pacific LLVP. The ULVZ is almost double the size of a previously imaged ULVZ in this region, but with half of the shear velocity reduction. This mega-sized ULVZ has accumulated due to stable mantle flow converging at the LLVP edge driven by slab-debris in the lower mantle. Such flow also develops the subvertical north-tilting edge of the Pacific LLVP.
The spatiotemporal neural dynamics of object location representations in the human brain
Authors: Graumann Monika , Ciuffi Caterina , Dwivedi Kshitij , Roig Gemma , Cichy Radoslaw M. 
Published: February 2022

AbstractTo interact with objects in complex environments, we must know what they are and where they are in spite of challenging viewing conditions. Here, we investigated where, how and when representations of object location and category emerge in the human brain when objects appear on cluttered natural scene images using a combination of functional magnetic resonance imaging, electroencephalography and computational models. We found location representations to emerge along the ventral visual stream towards lateral occipital complex, mirrored by gradual emergence in deep neural networks. Time-resolved analysis suggested that computing object location representations involves recurrent processing in high-level visual cortex. Object category representations also emerged gradually along the ventral visual stream, with evidence for recurrent computations. These results resolve the spatiotemporal dynamics of the ventral visual stream that give rise to representations of where and what objects are present in a scene under challenging viewing conditions.
Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics
Authors: Ratz Michael , von Berlin Leonie , Larsson Ludvig , Martin Marcel , Westholm Jakub Orzechowski , La Manno Gioele , Lundeberg Joakim , Frisén Jonas 
Published: February 2022

AbstractThe mammalian brain contains many specialized cells that develop from a thin sheet of neuroepithelial progenitor cells. Single-cell transcriptomics revealed hundreds of molecularly diverse cell types in the nervous system, but the lineage relationships between mature cell types and progenitor cells are not well understood. Here we show in vivo barcoding of early progenitors to simultaneously profile cell phenotypes and clonal relations in the mouse brain using single-cell and spatial transcriptomics. By reconstructing thousands of clones, we discovered fate-restricted progenitor cells in the mouse hippocampal neuroepithelium and show that microglia are derived from few primitive myeloid precursors that massively expand to generate widely dispersed progeny. We combined spatial transcriptomics with clonal barcoding and disentangled migration patterns of clonally related cells in densely labeled tissue sections. Our approach enables high-throughput dense reconstruction of cell phenotypes and clonal relations at the single-cell and tissue level in individual animals and provides an integrated approach for understanding tissue architecture.