bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Oncology Patient Engagement and Interest in Virtual Mind-Body Programming: Moving Toward Personalization of Virtual Care
Authors: Hung Tony K.W. , Latte-Naor Shelly , Li Yuelin , Kuperman Gilad J. , Seluzicki Christina , Pendleton Eva , Pfister David G. , Mao Jun J. 
Published: November 2022

PURPOSE: Accelerated by the COVID-19 pandemic, the virtual platform has become a prominent medium to deliver mind-body therapies, but the extent to which patients engage in virtual mind-body programming remains unclear. This study aims to assess oncology patient engagement in a virtual mind-body program. METHODS: We surveyed oncology patients enrolled in a live-streamed (synchronous) virtual mind-body program in May 2021. Patients self-reported engagement by weekly attendance. We applied multivariate regression to identify associations of engagement with sociodemographic and clinical factors. As an exploratory analysis, we used machine learning to partition engagement subgroups to determine preferential interest in prerecorded (asynchronous) mind-body therapy videos. RESULTS: Among 148 patients surveyed (response rate: 21.4%), majority were female (94.5%), White (83.1%), age 65 years or older (64.9%), retired (64.2%), and in survivorship (61.8%). Patient engagement ranged from 1 to 13 classes/week (mean [standard deviation]: 4.23 [2.56]) and was higher for female (β, .82; 95% CI, 0.01 to 1.62), non-White (β, .63; 95% CI, 0.13 to 1.13), and retired patients (β, .50; 95% CI, 0.12 to 0.88). The partition model identified three engagement subgroups: employed (low engagers), retired White (intermediate engagers), and retired non-White (high engagers). Particularly, low engagers had preferential interest in meditation videos (odds ratio, 2.85; 95% CI, 1.24 to 6.54), and both low and high engagers had preferential interest in Tai Chi videos (odds ratio, 2.26; 95% CI, 1.06 to 4.82). CONCLUSION: In this cross-sectional study among oncology patients, engagement in virtual mind-body programming was higher for female, non-White, and retired patients. Our findings suggest the need for both synchronous and asynchronous mind-body programming to meet the diverse needs of oncology patients.
Suppression of YAP safeguards human naïve pluripotency
Authors: Dattani Anish , Huang Tao , Liddle Corin , Smith Austin , Guo Ge 
Published: November 2022

ABSTRACT
Propagation of human naïve pluripotent stem cells (nPSCs) relies on the inhibition of MEK/ERK signalling. However, MEK/ERK inhibition also promotes differentiation into trophectoderm (TE). Therefore, robust self-renewal requires suppression of TE fate. Tankyrase inhibition using XAV939 has been shown to stabilise human nPSCs and is implicated in TE suppression. Here, we dissect the mechanism of this effect. Tankyrase inhibition is known to block canonical Wnt/β-catenin signalling. However, we show that nPSCs depleted of β-catenin remain dependent on XAV939. Rather than inhibiting Wnt, we found that XAV939 prevents TE induction by reducing activation of YAP, a co-factor of TE-inducing TEAD transcription factors. Tankyrase inhibition stabilises angiomotin, which limits nuclear accumulation of YAP. Upon deletion of angiomotin-family members AMOT and AMOTL2, nuclear YAP increases and XAV939 fails to prevent TE induction. Expression of constitutively active YAP similarly precipitates TE differentiation. Conversely, nPSCs lacking YAP1 or its paralog TAZ (WWTR1) resist TE differentiation and self-renewal efficiently without XAV939. These findings explain the distinct requirement for tankyrase inhibition in human but not in mouse nPSCs and highlight the pivotal role of YAP activity in human naïve pluripotency and TE differentiation.
This article has an associated ‘The people behind the papers’ interview.
Ubiquitin-assisted phase separation of dishevelled-2 promotes Wnt signalling
Authors: Vamadevan Vaishna , Chaudhary Neelam , Maddika Subbareddy 
Published: November 2022

ABSTRACT
Dishvelled-2 (Dvl2) is an essential component of Wnt pathway, which controls several cell fate decisions during development, such as proliferation, survival and differentiation. Dvl2 forms higher-order protein assemblies in the cell that are critical for relaying the signal from upstream Wnt ligand–frizzled receptor binding to downstream effector β-catenin activation. However, the precise molecular nature and contribution of Dvl2 protein assemblies during Wnt signalling is unknown. Here, we show that Dvl2 forms protein condensates driven by liquid–liquid phase separation. An intrinsically disordered region (IDR) at the N-terminus is essential for Dvl2 phase separation. Importantly, we identified the HECT-E3 ligase WWP2 as an essential driver of Dvl2 phase separation in vitro and in cells. We demonstrated that ubiquitylation of Dvl2 through K63 linkage by WWP2 is required for formation of Dvl2 condensates. Phase-separated Dvl2 activates Wnt signaling by sequestering the components of destruction complex and thus relieving β-catenin. Together, our results reveal a ubiquitylation-dependent liquid–liquid phase separation as a new process through which Dvl2 forms condensates, which is necessary for transduction of Wnt signalling.
This article has an associated First Person interview with the first author of the paper.
Non-invasive evaluation of the pathological and functional characteristics of chronic kidney disease by diffusion kurtosis imaging and intravoxel incoherent motion imaging: comparison with conventional DWI
Authors: Liang Ping , Yuan Guanjie , Li Shichao , He Kangwen , Peng Yang , Hu Daoyu , Li Zhen , Ma Zufu , Xu Chuou 
Published: November 2022

Objective:
To explore the diagnostic performance of diffusion kurtosis imaging (DKI) and incoherent intravoxel movement (IVIM) in evaluating the clinical and pathological characteristics in chronic kidney disease (CKD) compared to conventional diffusion-weighted imaging (DWI).

Methods:
Forty-nine CKD patients and 24 healthy volunteers were included in this retrospective study from September 2020 to September 2021. All participants underwent MRI examinations before percutaneous renal biopsy. Coronal T 2WI, axial T 1WI and T 2WI, and DWI (including IVIM and DKI) sequences obtained in one scan. We measured the apparent diffusion coefficient (ADC), true diffusion coefficient (Dt), pseudo-diffusion coefficient (Dp), perfusion fraction (fp), mean kurtosis (MK), and mean diffusivity (MD) values. One-way analysis of variance, correlation analysis, and receiver operating characteristic curve analysis were used in our study.

Results:
Cortex and medulla ADC, MK, Dt, fp were significantly different between the healthy volunteers and CKD stages 1–2 (all p &lt; 0.05). All diffusion parameters showed significant differences between CKD stages 1–2 and CKD stages 3–5 (all p &lt; 0.05). Except for the uncorrelation between MDMedulla and vascular lesion score, all other diffusion parameters were low-to-moderately related to clinical and pathological indicators. fpMedulla was the best parameter to differentiate healthy volunteers from CKD stages 1–2. MKCortex was the best parameter to differentiate CKD stages 1–2 from that CKD stages 3–5.

Conclusion:
Renal cortex and medulla fp, Dt, and MK can provide more valuable information than ADC values for the evaluation of clinical and pathological characteristics of CKD patients, and thus can provide auxiliary diagnosis for fibrosis assessment and clinical management of CKD patients.

Advances in knowledge:
IVIM and DKI can provide more diagnostic valuable information for CKD patients than conventional DWI.
‘What effect do safety culture interventions have on health care workers in hospital settings?’ A systematic review of the international literature
Authors: Finn Mairéad , Mellon Lisa , Walsh Aisling , O'Brien Niall , Williams David J. , Rafter Natasha , McCarthy Siobhán E. 
Published: November 2022

Introduction: Interventions designed to improve safety culture in hospitals foster organisational environments that prevent patient safety events and support organisational and staff learning when events do occur. A safety culture supports the required health workforce behaviours and norms that enable safe patient care, and the well-being of patients and staff. The impact of safety culture interventions on staff perceptions of safety culture and patient outcomes has been established. To-date, however, there is no common understanding of what staff outcomes are associated with interventions to improve safety culture and what staff outcomes should be measured. Objectives: The study seeks to examine the effect of safety culture interventions on staff in hospital settings, globally. Methods and Analysis: A mixed methods systematic review will be conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Searches will be conducted using the electronic databases of MEDLINE, EMBASE, CINAHL, Health Business Elite, and Scopus. Returns will be screened in Covidence according to inclusion and exclusion criteria. The mixed-methods appraisal tool (MMAT) will be used as a quality assessment tool. The Cochrane Collaboration’s tool for assessing risk of bias in randomised trials and non-randomised studies of interventions will be employed to verify bias. Synthesis will follow the Joanna Briggs Institute methodological guidance for mixed methods reviews, which recommends a convergent approach to synthesis and integration. Discussion: This systematic review will contribute to the international evidence on how interventions to improve safety culture may support staff outcomes and how such interventions may be appropriately designed and implemented.
THE EXTERNAL INFORMATIONAL BASIS ORGANIZATION OF THE PROJECT ACTIVITY OF GRADUATE STUDENTS ON THE DISCIPLINE “PROFESSIONAL FOREIGN LANGUAGE” IN THE FORM OF A HANDBOOK
Authors: Kabanova Natalya Yu. , Kabanov Vladimir S. , Khabibrakhmanova Farida R. 
Published: November 2022

Relevance. The purpose of mastering the subject “Professional English” for graduate students is ability to analyze, edit, create and translate professionally-oriented texts on their own, and to present the results of their scientific and professional activities. At the same time, there are usually no forms of such activities under the guidance of a teacher in the bachelor’s curriculum. Therefore, it is relevant to develop some methods and techniques of forming an informational basis for the self-dependent implementation of such activities.
Purpose. The aim of our work was to develop and test the external informational basis of graduate students’ educational activities in the form of a handbook and, with the help of various types of professionally oriented reading, to transform it into an internal informational basis that will allow them to write an abstract of a scientific article and to make a presentation on the topic of their research on their own, as well as to make a report.
Methods and hypotheses. The handbook was written within the framework of the project method. We assumed that the work on the handbook would increase the level of self-organization of educational activities and the quality of project activities of graduate students.
Results. As expected, the experiment proved our assumption: in the experimental group the percentage of timely completed educational tasks was higher, that indicates better self-organization of students. The grades for foreign-language presentations on the research topic, which are the main product of the project activities of graduate students, were also higher.
Practical implications. The results obtained prove the effectiveness of the handbook, and contribute to the practice of teaching foreign languages at the graduate students.
Professional stress and job satisfaction of nurses working in infectious diseases wards during the SARS-CoV-2 pandemic
Authors: Tomaszewska Katarzyna , Sopel Katarzyna 
Published: November 2022

The SARS-CoV-2 pandemic brings many challenges to the daily work of nurses who put their lives and health at risk for the general public every day. This causes them to be highly stressed at work, causes mental strain, and lowers the level of job satisfaction.
The aim of the research was to assess the level of stress and job satisfaction of nurses working in infectious diseases wards.
Materials and methods The survey was conducted among 169 nurses in the period from February to April 2022. The study was conducted using the diagnostic survey method, the technique was a questionnaire survey, and the survey tool was a survey questionnaire provided in a Google Form. The differences between the qualitative variables were analyzed using the χ2 test of independence. We use the χ2 test of independence (non-parametric significance test for two or more independent samples) to test whether the variables measured at the nominal level or higher in the comparison groups differ from each other. The significance level of the study was p <0.05. The calculations were made with the SPSS 25 software.
Results Work in direct contact with people infected with the coronavirus was performed by the majority of the respondents. Only about 18.0% of nurses used psychological assistance during the pandemic. Among the harmful and nuisance factors in the workplace, the respondents mentioned stress, contact with potentially infectious material, coronavirus infection despite appropriate personal protective equipment and cuts.
Conclusions Stress in the workplace was felt especially due to the need to operate specialized equipment, not yet operated by the respondents. Nurses in the workplace were mainly exposed to stress related to performing difficult and responsible activities. Only a small part of the respondents was definitely satisfied with their work.
Assessment of land use and land cover change on Gum Talha (Acacia seyal var. seyal) forest in Bahar Alarab, Sudan
Authors: YOUNIS AHMED YOUNIS IBRAHIM , JONATHAN BELLO NIYI , TOGUN ADENIYI OLUMUYIWA , YASIN EMAD HASSAN ELAWAD , OMER SIRAJ OSMAN , HAMAD GAMMER-ELDIEN ABDELRHMAN IBRAHIM , AÏSSATA SAMOURA DEMBA 
Published: November 2022

Abstract. Younis AYI, Jonathan BN, Togun AO, Yasin EHE, Omer SO, Hamad GAI, Aissata SD. 2022. Assessment of land use and land cover change on Gum Talha (Acacia seyal var. seyal) forest in Bahar Alarab, Sudan. Biodiversitas 23: 4549-4560. Evaluating land use and land cover change (LULC) is essential for the sustainable management of natural resources, biodiversity conservation, monitoring of food security, and research related to climate change and ecology. A better assessment of land-use changes is highly needed for further investigation due to increasingly rapid changes in LULC in response to human population growth. The emerging climatic change also has a significant effect on LULC. The objective of this study is to assess land use and land cover in Bahar Alarab, East Darfur State, Sudan, using remote sensing data obtained from satellite images. For assessing the LULC changes, Landsat images of the years 1988, 2002, and 2020 were downloaded and analyzed using QGIS 3.22.1 and ERDAS 2014 software, where supervised classification was applied with GPS point verification, change detection, matrix, and accuracy assessment. The analysis on LULC showed considerable changes during the two study periods, where 2020 had a considerable increase in forest cover in which on that year it occupied 26.44% of the area compared to 2002 and 1988 with 21.27% and 21.45%, respectively. Whereas 2002 the area was covered by a vast herbaceous vegetation (33.41%) compared to 1988 and 2002. Moreover, in 1988, shrubland decreased from 31.95% to 29.06% and 23.67% in 2020 and 2002, respectively. Sparse vegetation covered a considerable area in 2020 (23.61%) compared to 2002 and 1988 (21.65% and 17.71%, respectively). The results highlighted that there was statistically significant correlation between climate factors and LULC. Average temperature was highly positively correlated with sparse vegetation at 98.2%, while rainfall was highly negatively correlated with forest (-96.9%) and sparse vegetation (-88%), and highly positively correlated with herbaceous vegetation (83.5%). This study provides a unique understanding of LULC changes and their implications in management and conservation efforts, as well as a road map for decision makers for sustainable development of LULC in the Bahar Alarab.
The shifting genetic diversity pattern of Indonesian rice improved varieties from 1943-2019 based on historical pedigree data
Authors: ANAS ANAS , DAMAYANTI FARIDA , KADAPI MOHAMAD , CARSONO NONO , SARI SANTIKA 
Published: November 2022

Abstract. Anas, Damayanti F, Kadapi M, Carsono N, Sari S. 2022. The shifting genetic diversity pattern of Indonesian rice improved varieties from 1943-2019 based on historical pedigree data. Biodiversitas 23: 4649-4656. For rice plants in Indonesia, stagnation in increasing crop yields due to a reduction in genetic diversity is a significant issue. The issue of using the same parents in breeding programs and consumer preferences for a single main variety are among the causes of the narrowing of rice plants' genetic diversity. The purpose of this study is to figure out which ancestors are significant and how the genetic diversity of improved Indonesian rice cultivars has changed over time. Changes in the genetic background of the Indonesian rice gene pool were decided using pedigree analysis by calculating the coefficient of parentage (COP) among varieties. There are 280 ancestors in the rice gene pool. The pedigree map exemplifies the complexities of rice breeding in Indonesia. The four classical ancestors of DGWG, Taichung Native1, China, and Latisail had a noteworthy influence on all irrigated rice plant types (11.22%) and upland rice plant types (8.30%) in the gene pool. The dominance of the phenomenal variety IR64 has been continued by Inpari 32, which is a direct derivative of Ciherang. In the meantime, Inpago9, Luhur 2, and UPLRI ancestors set the foundation for the upland rice plant type. Inpara7 and Inpara9, along with their IRRI-introduced parents (IRRIpara4 and IRRIpara5), had a significant impact on Indonesian tidal rice plants. The A1, Hipa7, and Hipa3 varieties are heavily influenced by the hybrid rice plants of Indonesia.
Potential biocontrol agent of indigenous Bacillus sp. EG6.4: Molecular identification, larvicidal toxicity, and mechanism of actions
Authors: SALAMUN SALAMUN , SUSETYO RIZKY DANANG , NAFIDIASTRI FARAH AISYAH , ZAIN RIZKI AMALIAH , SARI ROSSY PERMATA , GERALDI ALMANDO , FATIMAH FATIMAH , NI’MATUZAHROH NI’MATUZAHROH 
Published: November 2022

Abstract. Salamun, Susetyo RD, Nafidiastri FA, Zain RA, Sari RP, Geraldi A, Fatimah, Ni’matuzahroh. 2022. Potential biocontrol agent of indigenous Bacillus sp. EG6.4: Molecular identification, larvicidal toxicity, and mechanism of actions Biodiversitas 23: 5431-5438. This research was carried out for molecular identification, as well as the determination and mechanism of action of larvicidal toxicity of Bacillus sp. EG6.4 isolated from breeding sites of Aedes aegypti from Gresik, East Java, Indonesia. Bacillus sp. EG6.4 was a Gram-positive endospore-forming bacteria. Molecular species identification using 16S rRNA gene sequencing showed that isolate had 97.89% similarity with Bacillus mojavensis. The isolate showed larvicidal toxicity against A. aegypti larvae. The lethal concentration 50% (LC50) values ??at 24 and 48hours exposure were 8.99±1.01 ×107 cells/mL and 8.43±1.01 ×107 cells/mL, respectively, while lethal time 50% (LT50) value was 11.9±1.1 hours. Production of chitinolytic enzymes or biosurfactants, chitinolytic and hemolytic assays were conducted to determine the larvicidal mechanism. As a result, Bacillus sp. EG6.4 showed hemolytic, but not chitinolytic activity, indicating its potency to produce biosurfactants. Transmission Electron Microscopy (TEM) result showed that isolate had oval-shaped endospores located subterminal with massive-shape parasporal inclusions. The detection of srfA-D gene showed that isolate produced surfactin biosynthesis thioesterase. Thus, Bacillus sp. EG6.4 produced biosurfactant that potentially to be developed as a biocontrol agent for disease vectors and plant pathogens.
A large Australian longitudinal cohort registry demonstrates sustained safety and efficacy of oral medicinal cannabis for at least two years
Authors: Vickery Alistair W. , Roth Sebastian , Ernenwein Tracie , Kennedy Jessica , Washer Patrizia 
Published: November 2022

Introduction
Oral medicinal cannabis (MC) has been increasingly prescribed for a wide range of clinical conditions since 2016. Despite an exponential rise in prescriptions and publications, high quality clinical efficacy and safety studies are lacking. The outcomes of a large Australian clinical electronic registry cohort are presented.

Methods
A prospective cannabis-naïve patient cohort prescribed oral MC participated in an ongoing longitudinal registry at a network of specialised clinics. Patient MC dose, safety and validated outcome data were collected regularly over two years and analysed.

Results
3,961 patients (mean age 56.07 years [SD 19.08], 51.0% female) with multimorbidity (mean diagnoses 5.14 [SD 4.08]) and polypharmacy (mean 6.26 medications [SD 4.61]) were included in this analysis. Clinical indications were for: chronic pain (71.9%), psychiatric (15.4%), neurological (2.1%), and other diagnoses (10.7%). Median total oral daily dose was 10mg for Δ9-tetrahydrocannabinol (THC) and 22.5mg for cannabidiol (CBD). A stable dose was observed for over two years. 37.3% experienced treatment related adverse events. These were graded mild (67%), moderate (31%), severe (<2%, n = 23) and two (0.1%) serious adverse events. Statistically significant improvements at a p value of <0.001 across all outcomes were sustained for over two years, including: clinical global impression (CGI-E, +39%: CGI-I, +52%; p<0.001), pain interference and severity (BPI, 26.1% and 22.2%; p<0.001), mental health (DASS-21, depression 24.5%, anxiety 25.5%, stress 27.7%; p<0.001), insomnia (ISI, 35.0%; p<0.001), and health status (RAND SF36: physical function, 34.4%: emotional well-being, 37.3%; p<0.001). Mean number of concomitant medications did not significantly change over 2 years (p = 0.481).

Conclusions
Oral MC was demonstrated to be safe and well-tolerated for a sustained period in a large complex cohort of cannabis-naïve, multimorbid patients with polypharmacy. There was significant improvement (p<0.001) across all measured clinical outcomes over two years. Results are subject to limitations of Real World Data (RWD) for causation and generalisability. Future high quality randomised controlled trials are awaited.