bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of dietary organic acid, probiotic and antioxidant on the growth performance and nutrient digestibility in growing rabbit
Authors: Chowdhury R , Rahman MA , Al Mamun M 
Published: July 2022

This study was conducted to compare the effects of different feed additives namely, organic acid (citric acid), probiotic (navio plus), and antioxidant (Bio-Sel-E) on the growth performance and nutrient digestibility in growing rabbit to convey a clear message on the use of one specific additive in rabbit diet. Thirty-two crossbred New Zealand White growing rabbit (four to five weeks of age) were randomly assigned to four different groups and reared for a period of fifty six days. Rabbit were fed on green grass (Hymenachne pseudointerrupta) and concentrate mixture (2703 Kcal ME/kg, 16.91 % CP). Additives were added at the top of the concentrate mixture, except for the control group. Compared with the control group, rabbit fed different additives showed 9–13% higher growth rate and 7–11% greater growth velocity; and probiotic supplemented group showed the best result (P<0.05) in terms of FCR value. Carcass weight (%) increased significantly in probiotic supplemented group, but numerically in organic acid and antioxidant supplemented groups compared with control group. Abdominal fat (%) was significantly decreased in organic acid, probiotic and antioxidant supplemented groups compared with control, however, lowest value (%) was recorded in probiotic supplemented group. Probiotic showed significant effect on pH decreasing trend in ingesta sample up to small intestine. Crude protein digestibility co-efficient (%) value was significantly higher in probiotic supplemented group, but numerically in organic acid and antioxidant supplemented groups compared with control group. Overall results indicated that, among the three different additives: organic acid, probiotic and antioxidant, probiotic may be considered as the best one for the better performance and nutrient digestibility in growing rabbit.
Bangladesh Journal of Animal Science, 51 (2): 55-61.
Effect of Napier silage on milk production of Holstein Friesian Crossbred Cow
Authors: Habib MAA , Akbar MA , Khandaker ZH 
Published: July 2022

This work aspired to evaluate the quality of different types of silage on milk production made from whole napier grass, napier stem, and napier leaf. Napier grass (Pennisetum Purpureum) was harvested at 70 days of age and made into three types of silage in three different silo pits under proper anaerobic conditions for 45 days. A feeding program of 60 days duration was designed on nine (9) lactating (110-140 days after calving) Holstein Friesian crossbred dairy cows of 2nd parity (age 6-7 years, live weight 500±5 kg). The cows were divided into 3 groups and assigned to 3 dietary treatments, basal ingredients with whole napier grass silage (T1), basal ingredients with chopped napier leaf silage (T2), and basal ingredients with napier stem silage (T3). After ensiling, it was found that napier stem silage possesses the highest pH value indicating low-quality silage whereas napier leaf silage possesses the lowest pH value. The pH value of whole napier grass silage was slightly higher than that of napier leaf silage. Concentrations of CP and NH3-N were highest in napier leaf silage and lowest in napier stem silage. The CP value was almost similar in whole napier grass silage and napier leaf silage but the NH3-N value of whole napier grass silage was lower than that of napier leaf silage. Among the three groups, the cows of napier leaf silage group gave significantly (P<0.05) higher milk yield than the cows of whole napier grass and napier stem silage group. On the other hand, the cows of napier stem silage group gave significantly (P<0.05) lower milk yield than the cows of whole napier grass and napier leaf silage group. It can be concluded that napier leaf and whole napier grass silage are comparatively better than napier stem silage in respect to milk production of dairy cows.
Bangladesh Journal of Animal Science, 51 (2): 62-67.
Sensorial, chemical and microbial quality of spongy rosogolla
Authors: Hossain MS , Khandakar MMH , Islam MN , Islam MA 
Published: July 2022

The goal of this investigation was to compare the physical, chemical and microbial qualities of laboratory made and market spongy rosogolla. Two types of spongy rosogolla were made in laboratory from the cow and buffalo milk chhana to reveal the quality in regards of the species and three types of market spongy rosogolla were collected from three different shops. With regard to the physical attributes, laboratory made spongy rosogolla were found better than the market spongy rosogolla and cow milk spongy rosogolla was the best. In chemical aspects, laboratory made spongy rosogolla had greater pH, fat, protein, and ash content, whereas, market spongy rosogolla showed higher acidity and carbohydrate content. The moisture content of cow milk spongy rosogolla was highest. The mineral contents (Ca, P, and Mg) were also differed significantly (p<0.01) among the samples except for Na content. Highest Ca, P and Mg contents were found in market spongy rosogolla. The total viable count (TVC) was significantly (p<0.01) lower in laboratory made spongy rosogolla. However, the Escherichia coli count was found similar (p>0.05) in both cow and buffalo milk spongy rosogolla but lower (p>0.05) than other market spongy rosogolla samples. Considering all the findings, it may be concluded that the quality of market spongy rosogolla needs improvement and responsible authorities might take necessary initiatives for monitoring.
Bangladesh Journal of Animal Science, 51 (2): 81-84.
Spiritualitas dengan Stres Mahasiswa yang Melakukan Praktik Keperawatan di Masa Pandemi Covid-19
Authors: Sinurat Samfriati , Simbolon Pomarida , Zega Besty Apriani 
Published: July 2022

Pandemi Corona Virus-19 Disease (Covid-19) yang tidak dapat diprediksi kapan selesainya, berdampak pada sektor kehidupan pendidikan mahasiswa dalam pendidikan keperawatan didalam pengalaman belajar praktek. Beberapa studi menunjukkan krisis Covid-19 dapat meningkatkan masalah serius pada kesejahteraan psikologis mahasiswa profesi ners seperti ketakutan terinfeksi atau tanpa sadar menginfeksi orang lain. Secara positif kesejahteraan psikologis sangat erat hubungannya dengan spiritualitas sebagai motivasi, kekuatan yang mendorong, mengarahkan dan memilih perilaku mahasiswa sehingga menemukan keharmonisan dan ketenangan hidup sehingga akhirnya mengerti makna dan tujuan dan perannya sebagai mahasiswa perawat. Tujuan dari penelitian untuk mengidentifikasi hubungan spiritualitas dengan stres mahasiswa pada mahasiswa profesi dalam praktek keperawatan di masa pandemi Covid-19 pada bulan April 2021. Rancangan penelitian analitik dengan pendekatan cross sectional. Sampel penelitian adalah mahasiswa profesi ners STIKes Santa Elisabeth Medan tahun 2021 berjumlah 84 responden dengan teknik total sampling. Instrumen penelitian menggunakan kuesioner DASS 42 untuk stres dengan pendekatan survey online, data dianalisis dengan uji chi-square. Hasil penelitian menunjukkan bahwa sebagian besar responden memiliki spritualitas sedang (39,3%) dengan tingkat stres sedang (41,7%). Hasil uji statistik menunjukkan nilai p = 0,001 dengan nilai signifikan p = < 0,05. Secara statistik terdapat hubungan yang signifikan antara spiritualitas dengan tingkat stres mahasiswa profesi ners yang melakukan praktik keperawatan di masa pandemi Covid-19 tahun 2021. Responden perlu dukungan eksternal baik dari keluarga maupun tim kesehatan lainnya agar lebih yakin terhadap kemampuan dirinya yang memudahkan mahasiswa dalam mengatasi stres dan meningkatkan spiritualitasnya sehinga dapat melaksanakan belajar prakteknya dengan sehat dan sejahtera.
Aktivitas Antioksidan Total pada Ekstrak Etanol Daun Bambu Surat (Gigantochloa pseudoarundinaceae)
Authors: Mamay Mamay , Wardani Diah , Hakim Fathul 
Published: July 2022

Antioksidan merupakan senyawa yang dapat menghambat reaksi oksidasi, dengan cara mengikat radikal bebas dan molekul yang sangat reaktif dalam tubuh manusia Bambu surat (G. pseudoarundinaceae) adalah salah satu jenis tanaman bambu epidemik Indonesia yang diketahui mengandung senyawa antioksidan. Tujuan dari penelitian ini adalah untuk mengetahui gambaran aktivitas antioksidan total dalam ekstrak etanol daun muda, tua dan sangat tua bambu surat, dengan metode spektrofotometri. Jenis penelitian yang digunakan adalah deskriptif laboratorik dengan pemeriksaan laboratorium secara kualitatif dan kuantitatif. Sampel yang diambil dalam penelitian ini yaitu daun muda, tua dan sangat tua. Pengambilan sampel dilakukan dengan teknik purposive sampling. Pengolahan sampel dengan cara ekstraksi dengan metode meserasi cara dingin menggunakan etanol. Penetapan kadar antioksidan dilakukan dengan menggunakan spektrofotometri dan data yang didapat diolah dengan perhitungan persentasi inhibisi, IC50 (inhibitory concentration), AAI (antioxidant activity index). Pada ekstrak daun muda memiliki nilai IC50 57,46 ppm, AAI 2.78; dan pada ekstrak daun tua memiliki nilai IC50 42,02 ppm dan AAI 3.81 (AAI>2 antioksidan bersifat sangat kuat). Sedangkan ekstrak daun sangat tua memiliki nilai IC50 123,80 ppm dan AAI 1.29 (AAI>I antioksidan bersifat kuat. Lebih lanjut, Kuercetin dan vitamin C sebagai pembanding memiliki nilai IC50 dan AAI sebesar 33,74 (AAI=4,74) dan 37,91 (AAI=4,22).
Xiong Fu Powder Regulates the Intestinal Microenvironment to Protect Bones Against Destruction in Collagen-Induced Arthritis Rat Models
Authors: Xi Xiaoyu , Ye Qinbin , Li Xiaoya , Lu Xiangchen , Fan Danping , Xia Ya , Xiao Cheng 
Published: July 2022

BackgroundChanges in the intestinal microenvironment affected bone destruction in rheumatoid arthritis (RA), and spleen deficiency (SD) was closely related to the intestinal microenvironment. In this study, we aimed to explore the aggravation of SD on collagen-induced arthritis (CIA) and the bone protection of compound Xiong Fu powder (XFP) on CIA with SD (SD-CIA) based on the intestinal microenvironment.MethodAn SD-CIA rat model was established using Rheum officinale Baill. decoction combined with CIA and then treated with XFP. The aggravating action of SD on CIA rats and the efficacy of XFP were evaluated using AI scores, H&amp;E staining of the joint, and level of serum anti–collagen type II antibody (Col II Ab). Bone destruction was assessed by micro-CT and TRACP staining. In addition, flow cytometry, qRT-PCR, and ELISA were used to evaluate gut mucosal immunity. Moreover, metagenomic sequencing was used to determine the distribution and function of the gut microbiota.ResultsCompared with that in CIA rats, bone destruction in SD-CIA rats was aggravated, as manifested by increased AI scores, more severe joint pathological changes and radiological damage, and increased number of osteoclasts (OCs) in the ankle joint. Meanwhile, the proportion of Tregs/Th17 cells was biased toward Th17 cells in Peyer’s patches. Furthermore, the gene levels of TNF-α, IL-1β, IL-6, and IL-17 were increased. In contrast, the expression of IL-10 and sIgA was decreased in the jejunum and ileum. XFP treatment improved bone damage and intestinal mucosal immune disorders compared with the SD-CIA group. In addition, the distribution and function of the gut microbiota were altered in the SD-CIA group. After XFP treatment, the community and function of the gut microbiota were regulated, manifested as increased abundance of several Lactobacillus species, such as L. acidophilus, which regulates the intestinal Tregs/Th17 cells and quorum sensing pathways, followed by promoting probiotic adhesion to the intestines.ConclusionSD can aggravate bone destruction in CIA rats. Compound XFP may attenuate bone destruction in SD-CIA rats by regulating the intestinal microenvironment. One of the mechanisms is the cross-talk between sIgA secretion regulated by intestinal mucosal Tregs and Th17 cells and adhesion of Lactobacillus mediated by quorum sensing.
Development of Prediction Model to Estimate the Risk of Heart Failure in Diabetes Mellitus
Authors: Qu Hongling , Wu Cuiyun , Ye Peiji , Lv Weibiao 
Published: July 2022

BackgroundHeart failure (HF) is a leading cause of mortality and disability in patients with diabetes mellitus (DM). The aim of the study is to predict the risk of HF incidence in patients with DM by developing a risk prediction model.MethodsWe constructed a regression model based on 270 inpatients with DM between February 2018 and January 2019. Binary logistic regression was applied to develop the final model incorporating the predictors selected by least absolute shrinkage and selection operator regression. The nomogram was estimated with an area under the receiver operator characteristic curve and calibration diagram and validated with the bootstrap method.ResultsRisk factors including age, coronary heart disease (CHD), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) were incorporated in the final model as predictors. Age ≥ 61 years old, LDL, and CHD were risk factors for DM with HF, with odds ratios (ORs) of 32.84 (95% CI: 6.74, 253.99), 1.33 (95% CI: 1.06, 1.72), and 3.94 (95% CI: 1.43, 13.43), respectively. HDL was a protective factor with an OR of 0.11 (95% CI: 0.04, 0.28). The area under curve of the model was 0.863 (95% confidence interval, 0.812∼0.913). The plot of the calibration showed that there was a good consistency between predicted probability and actual probability. Harrell’s C-index of the nomogram was 0.845, and the model showed satisfactory calibration in the internal validation cohort.ConclusionThe prediction nomogram we developed can estimate the possibility of HF in patients with DM according the predictor items.
Left Ventricular Diastolic Dysfunction Is Not Associated With Pulmonary Edema in Septic Patients. A Prospective Observational Cohort Study
Authors: Kahl Ursula , Schirren Leah , Yu Yuanyuan , Lezius Susanne , Fischer Marlene , Menke Maja , Sinning Christoph , Nierhaus Axel , Vens Maren , Zöllner Christian , Kluge Stefan , Goepfert Matthias S. , Roeher Katharina 
Published: July 2022

PurposeWe aimed to investigate whether left ventricular diastolic dysfunction (LVDD) is associated with pulmonary edema in septic patients.MethodsWe conducted a prospective cohort study in adult septic patients between October 2018 and May 2019. We performed repeated echocardiography and lung ultrasound examinations within the first 7 days after diagnosis of sepsis. We defined LVDD according to the 2016 recommendations of the American Society of Echocardiography and—for sensitivity analysis—according to an algorithm which has been validated in septic patients. We quantified pulmonary edema using the lung ultrasound score (LUSS), counting B-lines in four intercostal spaces.ResultsWe included 54 patients. LVDD was present in 51 (42%) of 122 echocardiography examinations. The mean (±SD) LUSS was 11 ± 6. There was no clinically meaningful association of LVDD with LUSS (B = 0.55 [95%CI: −1.38; 2.47]; p = 0.571). Pneumonia was significantly associated with higher LUSS (B = 4.42 [95%CI: 0.38; 8.5]; p = 0.033).ConclusionThe lack of a clinically meaningful association of LVDD with LUSS suggests that LVDD is not a major contributor to pulmonary edema in septic patients.Trial RegistrationNCT03768752, ClinicalTrials.gov, November 30th, 2018 - retrospectively registered.
Non-Linear Correlation Between Tumor Size and Survival Outcomes for Parathyroid Carcinoma: A SEER Population-Based Cohort Study
Authors: Zhang Kun , Su Anping , Wang Xiaofei , Zhao Wanjun , He Linye , Wei Tao , Li Zhihui , Zhu Jingqiang , Chen Ya-Wen 
Published: July 2022

BackgroundParathyroid carcinoma (PC) is a rare malignancy without a commonly acknowledged prognostic assessment and treatment system. This study captures how independent prognostic factors and tumor size correlate with outcomes in patients with PC.MethodsThe Surveillance, Epidemiology, and End Results database was used to perform a retrospective analysis on PC patients from 2000 to 2018. Univariate and multivariable survival analyses were performed to evaluate cancer-specific survival (CSS) and overall survival (OS), to identify independent prognostic factors in the PC patient population. A generalized additive model was applied to conduct smooth curve fitting and to examine the association between tumor size and relative risk of death.ResultsA total of 590 patients were included. The 5- and 10-year OS were 80.8% and 67.1%, respectively. 5- and 10-year CSS was estimated to be 93.6% and 92.1%, respectively. The association between tumor size and relative risk of death can be generalized as a U-shaped curve. The mortality risk reaches its lowest point when tumor diameter approaches 2cm. At a tumor diameter cutoff of 3cm for CSS and 4cm for OS, there is an abrupt drop in survival rates. Multivariate Cox analysis revealed age, no surgery, and debulking surgery as consistent predictors of lower OS and CSS.ConclusionsA non-linear correlation between tumor size and death risk has been identified in patients with PC, along with an accurate size threshold at which survival rates sharply decrease. Further investigation is needed to determine if these trends are seen in other malignancies with promising prognoses.
The Lung Alveolar Cell (LAC) miRNome and Gene Expression Profile of the SP-A-KO Mice After Infection With and Without Rescue With Human Surfactant Protein-A2 (1A0)
Authors: Thorenoor Nithyananda , Floros Joanna 
Published: July 2022

Human surfactant protein (SP)-A1 and SP-A2 exhibit differential qualitative and quantitative effects on the alveolar macrophage (AM), including a differential impact on the AM miRNome. Moreover, SP-A rescue (treatment) of SP-A-knockout (KO) infected mice impoves survival. Here, we studied for the first time the role of exogenous SP-A protein treatment on the regulation of lung alveolar cell (LAC) miRNome, the miRNA-RNA targets, and gene expression of SP-A-KO infected mice of both sexes. Toward this, SP-A-KO mice of both sexes were infected withKlebsiella pneumoniae, and half of them were also treated with SP-A2 (1A0). After 6 h of infection/SP-A treatment, the expression levels and pathways of LAC miRNAs, genes, and target miRNA-mRNAs were studied in both groups. We found 1) significant differences in the LAC miRNome, genes, and miRNA-mRNA targets in terms of sex, infection, and infection plus SP-A2 (1A0) protein rescue; 2) an increase in the majority of miRNA-mRNA targets in both study groups in KO male vs. female mice and involvement of the miRNA-mRNA targets in pathways of inflammation, antiapoptosis, and cell cycle; 3) genes with significant changes to be involved in TP-53, tumor necrosis factor (TNF), and cell cycle signaling nodes; 4) when significant changes in the expression of molecules from all analyses (miRNAs, miRNA-mRNA targets, and genes) were considered, two signaling pathways, the TNF and cell cycle, referred to as “integrated pathways” were shown to be significant; 5) the cell cycle pathway to be present in all comparisons made. Because SP-A could be used therapeutically in pulmonary diseases, it is important to understand the molecules and pathways involved in response to an SP-A acute treatment. The information obtained contributes to this end and may help to gain insight especially in the case of infection.
Risk, Incidence, and Mortality of Breast Cancer in Primary Sjögren’s Syndrome: A Systematic Review and Meta-Analysis
Authors: Deng Jian , Liu Mengsi , Xiao Ruoyi , Wang Jin , Liao Xibei , Ye Zhen , Sun Zhen 
Published: July 2022

BackgroundPrimary Sjögren’s syndrome (pSS) and breast cancer are a highly prevalent autoimmune disease and malignancy, respectively, both occurring predominantly in females. Whether there is a link between these two diseases is uncertain. We conducted a systematic review and meta-analysis to investigate the risk, incidence, and mortality of breast cancer in patients with pSS.MethodsWe systematically searched Embase, PubMed, and Web of Science on January 31, 2022 to identify the study that assessed risk, incidence, or mortality of breast cancer in pSS. The fixed or random-effects models were applied to pool the effect estimates based on heterogeneity measured by Cochran’s Q-test and Higgins’ I2.ResultsTen studies involving 725,805 participants and 64,836 pSS patients were included in our analysis. The pooled result showed that, overall, pSS was not associated with the risk (SIR=0.92, 95%CI: 0.66-1.29, P=0.646) and mortality (HR = 0.78, 95%CI: 0.26-2.34, P = 0.664) of breast cancer; however, when stratified by geographic region, we found that patients with pSS in Asian countries (SIR=1.32, 95%CI: 1.10-1.58, P=0.003) and Argentina (SIR=3.76, 95%CI: 1.04-9.45, P=0.019) had an elevated risk of breast cancer, while pSS in Europe was associated with a reduced risk (SIR=0.61, 95%CI: 0.51-0.73, P&lt;0.001). The pooled result from 28,635 female pSS patients indicated that the incidence of breast cancer was 2.15 (95% CI: 1.33-3.50) per 1000 person/years.ConclusionThis study suggests that there may be geographical differences in the association between pSS and breast cancer risk; patients with pSS in European countries are associated with a lower risk of breast cancer, while Asia and Argentina are the opposite. Future research is needed to further characterize the effect of pSS on breast cancer risk and the pathophysiological mechanisms underlying this association to unravel the complex relationship between the two.
mCRP as a Biomarker of Adult-Onset Still’s Disease: Quantification of mCRP by ELISA
Authors: Fujita Chitose , Sakurai Yasuo , Yasuda Yuki , Homma Rino , Huang Cheng-Long , Fujita Masaaki 
Published: July 2022

BackgroundC-reactive protein (CRP) is a dynamic protein that undergoes conformational changes between circulating native pentameric CRP (pCRP), pentameric symmetrical forms (pCRP*) and monomeric (or modified) CRP (mCRP) forms. mCRP exhibits strong pro-inflammatory activity and activates platelets, leukocytes, and endothelial cells. Abundant deposition of mCRP in inflamed tissues plays a role in several disease conditions, such as ischemia/reperfusion injury, Alzheimer’s disease, and cardiovascular disease. Although pCRP is typically quantified rather than mCRP for clinical purposes, mCRP may be a more appropriate disease marker of inflammatory diseases. Therefore, simple methods for quantifying mCRP are needed.MethodsWe developed a specific enzyme-linked immunosorbent assay (ELISA) to measure plasma levels of mCRP. Plasma mCRP concentration was measured in patients with adult-onset Still’s disease (AOSD) (n=20), polymyalgia rheumatica (PMR) (n=20), rheumatoid arthritis (RA) (n=30), infection (n=50), and in control subjects (n=30) using the developed ELISA.ResultsWe demonstrated that mCRP is elevated in some inflammatory autoimmune diseases, particularly AOSD. The mCRP concentration was also significantly higher among AOSD patients than RA, PMR patients and controls (477 ng/ml, 77 ng/ml, 186 ng/ml, and 1.2 ng/ml, respectively). Also, the mCRP (×1,000)/pCRP ratio was significantly higher among AOSD patients than RA, PMR, and infection patients (3.5, 0.6, 1,6, and 2.0, respectively).ConclusionThe plasma mCRP levels are elevated in some autoimmune diseases, particularly AOSD. The plasma mCRP levels may therefore be a potentially useful biomarker for AOSD.