bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using Simulation to Teach Learners in Health Care Behavioral Skills Related to Diversity, Equity, and Inclusion
Authors: Daya Sneha , Illangasekare Tushani , Tahir Peggy , Bochatay Naike , Essakow Jenna , Ju Mindy , van Schaik Sandrijn 
Published: October 2022

Summary Statement
Bias is commonplace in the health care environment and can negatively impact patients and their health outcomes. Simulation has long been shown to be an effective teaching tool for communication skills in health care, but it has rarely been used to deliver concrete behavioral skills that address issues of diversity, equity, and inclusion (DEI). This scoping review examines 23 published articles surrounding the use of simulation in health care education to impart behavioral skills that reduce bias and promote DEI. Included articles described various behavioral skills including communication, history-taking, and system/community-level advocacy. The most commonly used simulation modality to teach these skills included the use of simulated participants (16 articles, 70%). The main DEI topics addressed in the trainings included sexual orientation/gender identity, language, and culture/ethnicity. Based on findings from this review, the authors suggest recommendations for educators who are considering teaching DEI-related skills through simulation.
Does Exercise Training Improve Physical Fitness and Health in Adult Liver Transplant Recipients? A Systematic Review and Meta-analysis
Authors: De Smet Stefan , O’Donoghue Katriona , Lormans Maud , Monbaliu Diethard , Pengel Liset 
Published: October 2022

Background.
The impaired physical fitness of end-stage liver disease patients often persists after liver transplantation (LT) and compromises posttransplant recovery. This systematic review and meta-analysis evaluated evidence supporting the potential of exercise training to improve physical fitness and health-related quality of life (HRQOL) after LT.

Methods.
Bibliographic searches identified all randomized controlled trials (RCTs) comparing aerobic and strength training versus usual care after LT. Risk of bias was assessed, and study outcomes measuring physical fitness and HRQOL were extracted. Meta-analysis was performed if at least 3 studies reported on an outcome.

Results.
Eight RCTs (n = 334) were identified. Methodological study quality varied and was poorly reported. Meta-analyses showed a trend for favorable effects of exercise on cardiorespiratory fitness (peak oxygen uptake or 6-min walking distance; 6 studies, n = 275; standardized mean difference: 0.23, 95% confidence interval [CI], −0.01 to 0.48) and of strength training either or not combined with aerobic training on muscular fitness (dynamometry-assessed muscle strength or 30-s sit-to-stand test; 3 studies, n = 114; standardized mean difference: 0.34, 95% CI, −0.03 to 0.72). A favorable effect was found for exercise on the Short-Form Health Survey-36 HRQOL physical function subcomponent (3 studies, n = 194; mean difference: 9.1, 95% CI, 0.3-17.8). No exercise-related adverse events were observed.

Conclusions.
RCTs indicate that exercise training in LT recipients is safe, improves physical function aspects of HRQOL, and may benefit cardiorespiratory and muscular fitness. The strength of evidence is, however, limited by the low number of patients and study quality. More adequately powered, high-quality RCTs are warranted.
Impact of the Kidney Transplantation Moratorium in France Because of the COVID-19 Pandemic: A Cohort-based Study
Authors: Bonnemains Vincent , Le Borgne Florent , Savoye Emile , Legeai Camille , Pastural Myriam , Bayat-Makoei Sahar , Lenain Rémi , Ragot Stéphanie , Leffondré Karen , Couchoud Cécile , Foucher Yohann 
Published: October 2022

Background.
The COVID-19 pandemic has resulted in worldwide kidney transplantation (KT) moratoriums. The impacts of these moratoriums on the life expectancy of KT candidates remain unclear.

Methods.
We simulated the evolution of several French candidate populations for KT using a multistate semi-Markovian approach and according to moratorium durations ranging from 0 to 24 mo. The transition rates were modeled from the 63 927 French patients who began dialysis or were registered on the waiting list for KT between 2011 and 2019.

Results.
Among the 8350 patients active on the waiting list at the time of the French KT moratorium decided on March 16, 2020, for 2.5 mo, we predicted 4.0 additional months (confidence interval [CI], 2.8-5.0) on the waiting list and 42 additional deaths (CI, –70 to 150) up to March 16, 2030, compared with the scenario without moratorium. In this population, we reported a significant impact for a 9-mo moratorium duration: 135 attributable deaths (CI, 31-257) up to March 16, 2030. Patients who became active on the list after March 2020 were less impacted; there was a significant impact for an 18-mo moratorium (175 additional deaths [CI, 21-359]) in the 10 862 prevalent end-stage renal disease patients on March 16, 2020 and for a 24-mo moratorium (189 additional deaths [CI, 10-367]) in the 16 355 incident end-stage renal disease patients after this date.

Conclusion.
The temporary moratorium of KT during a COVID-19 peak represents a sustainable decision to free up hospitals’ resources if the moratorium does not exceed a prolonged period.
Green spaces for mental disorders
Authors: Wood Carly , Wicks Claire , Barton Jo 
Published: October 2022

Purpose of review
Mental illness is a global challenge, exacerbated by the coronavirus pandemic. Research suggests access to local green spaces is associated with better mental health, yet access is not always equitable. Evaluation of how nature-based interventions protect and support mental health is therefore required.

Recent findings
Accessible local green spaces are associated with better mental health. They encourage active behaviours and social interaction, reduce loneliness and stress. Green views from the home are associated with increased self-esteem, life satisfaction and happiness and reduced depression, anxiety and loneliness. Nature-based interventions and green social prescriptions effectively target vulnerable groups, resulting in significant reductions in depression, anxiety and anger alongside positive mental health outcomes.

Summary
Although existing evidence is encouraging, robust, high-quality research that strengthens the evidence base and informs future clinical practice and policy decision making is needed. Evidence of the long-term effectiveness in individuals with diagnosed mental illness is also required to ascertain the potential social and wider returns on investment. Barriers to use of green social prescriptions like mental health symptoms and geographical accessibility need to be overcome to increase accessibility and uptake of green social prescriptions for the prevention and treatment of mental illness.
Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes
Authors: Moreno-Echeverri Aura Maria , Susnik Eva , Vanhecke Dimitri , Taladriz-Blanco Patricia , Balog Sandor , Petri-Fink Alke , Rothen-Rutishauser Barbara 
Published: October 2022

AbstractAbstract FigureBackgroundIn the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental outcome. We address some of these pitfalls and suggest possible solutions. The accuracy of fluorescence microscopy methods has an important role in obtaining insights into NP interactions with lysosomes at the single cell level including quantification of NP uptake in a specific cell type.MethodsHere we use J774A.1 cells as a model for professional phagocytes. We expose them to fluorescently-labelled amorphous silica NP with different sizes and quantify the colocalization of fluorescently-labelled NP with lysosomes over time. We focus on confocal laser scanning microscopy (CLSM) to obtain 3D spatial information and follow live cell imaging to study NP colocalization with lysosomes.ResultsWe evaluate different experimental parameters that can bias the colocalization coefficients (i.e, Pearson’s and Manders’), such as the interference of phenol red in the cell culture medium with the fluorescence intensity and image post-processing (effect of spatial resolution, optical slice thickness, pixel saturation and bit depth). Additionally, we determine the correlation coefficients for NP entering the lysosomes under four different experimental set-ups. First, we found out that not only Pearson’s, but also Manders’ correlation coefficient should be considered in lysosome-NP colocalization studies; second, there is a difference in NP colocalization when using NP of different sizes and fluorescence dyes and last, the correlation coefficients might change depending on live-cell and fixed-cell imaging set-up.ConclusionsThe results summarize detailed steps and recommendations for the experimental design, staining, sample preparation and imaging to improve the reproducibility of colocalization studies between the NP and lysosomes.
Impact of Fluvoxamine on outpatient treatment of COVID-19 in Honduras
Authors: Pineda Estela , Singh Jarmanjeet , Pineda Miguel Vargas , Umanzor Jose Garay , Baires Fernando , Benitez Luis G. , Burgos Cesar , Sekhon Anupamjeet Kaur , Crisp Nicole , Lewis Anita S. , Radwanski Jana , Bermudez Marco , Barjun Karen Sanchez , Diaz Oscar , Palou Elsa , Escalante Rossany E. , Hernandez Carlos Isai , Stevens Mark L. , Eberhard Deke , Sierra Manuel , Alvarado Tito , Videa Omar , Sierra-Hoffman Miguel , Pascua Fernando Valerio 
Published: October 2022

AbstractBackgroundCOVID-19 pandemic has impacted lives globally. While COVID-19 did not discriminate against developed or developing nations, it has been a significant challenge for third world countries like Honduras to have widespread availability of advanced therapies. The concept of early treatment was almost unheard-of when early outpatient treatment with repurposed drugs in Latin American countries showed promising results. One such drug is fluvoxamine, that has shown tremendous potential in two major studies, following which fluvoxamine was added to the standard of care in Honduras.MethodsThis is a prospective observational study performed at the Hospital Centro Médico Sanpedrano (CEMESA) in San Pedro Sula, Cortes, Honduras in the COVID-19 outpatient clinic. All patients fifteen years of age or older, with mild or moderate signs and symptoms of COVID-19, and a positive SARS-CoV-2 antigen or Reverse Transcription Polymerase Chain Reaction (RT-PCR) were included in the study and prescribed fluvoxamine. Cohort of patients who decided to take fluvoxamine were compared to the cohort who did not take fluvoxamine for mortality risk and risk of hospitalization as primary endpoints. Patient were monitored for 30 days with first follow up at 7 days and second follow up at 10-14 days of symptom onset. Categorical variables were compared by Pearson Chi-square test. The Odds ratio was calculated using univariate and multivariate logistic regression. Continuous variables were compared by t-test and Wilcoxon rank-sum tests.ResultsOf 657 total COVID-19 cases, 594 patients took fluvoxamine and 63 did not. A total of five patients (0.76 percent) died, of which only one death occurred in the fluvoxamine group. Patients who did not receive fluvoxamine had a significantly higher mortality (OR 24, p0.005, CI 2.6 to 233.5). Odds ratio of hospitalization in patients who did not take fluvoxamine was 2.38 (30 vs 10 hospitalizations, p 0.040, CI 1.04-5.47). The odds ratio of requiring oxygen in patients in the non-fluvoxamine group was 5.08 (p<0.001, CI 2.18-11.81). Mean lymphocytes count on the first follow-up visit was significantly higher in the fluvoxamine group (1.72 vs. 1.38, Δ 0.33, p 0.007, CI 0.09 to 0.58).ConclusionThe results of our study suggest lowers odds of mortality and hospitalization in patients who took fluvoxamine vs fluvoxamine non-takers. Non-fluvoxamine group had higher odds of oxygen requirement than fluvoxamine group as well.
Condition-dependence resolves the paradox of missing plasticity costs
Authors: De Lisle Stephen P. , Rowe Locke 
Published: October 2022

AbstractPhenotypic plasticity plays a key role in adaptation to changing environments. However, plasticity is neither perfect nor ubiquitous, implying that fitness costs must limit the evolution of phenotypic plasticity in nature. The measurement of such costs of plasticity has proved elusive; decades of experiments show that fitness costs of plasticity are often weak or nonexistent. Here, we show that this paradox can be at least partially explained by condition-dependence. We develop two models differing in their assumptions about how condition-dependence arises; both models show that variation in condition can readily mask costs of plasticity even when such costs are substantial. This can be shown simply in a model where costly plasticity itself evolves condition-dependence. Yet similar effects emerge from an alternative model where trait expression is condition-dependent. In this more complex model, average condition in each environment and genetic covariance in condition across environments both determine when costs of plasticity can be revealed. Analogous to the paradox of missing trade-offs between life history traits, our models show that variation in condition masks costs of plasticity even when costs exist, and suggests this conclusion may be robust to the details of how condition affects trait expression. Our models demonstrate that condition dependence can also account for the often-observed pattern of elevated plasticity costs inferred in stressful environments, the maintenance of genetic variance in plasticity, and provides insight into experimental and biological scenarios ideal for revealing a cost of phenotypic plasticity.
Gains and losses of the epiphytic lifestyle in epidendroid orchids: review and new analyses with succulence traits
Authors: Collobert Géromine , Perez-Lamarque Benoît , Dubuisson Jean-Yves , Martos Florent 
Published: October 2022

ABSTRACTBackground and AimsEpiphytism has evolved repeatedly in plants and has resulted in a considerable number of species with original characteristics. Succulent forms in particular are thought to have evolved as an adaptation to the epiphytic environment, because the water supply is generally erratic compared to soils’. However, succulent organs also exist in terrestrial plants, and the question of the concomitant evolution of epiphytism and succulence has received little attention, not even in the epidendroid orchids, which account for 68% of vascular epiphytes.MethodsWe reconstructed a new time-calibrated phylogenetic tree of Epidendroideae with 203 genera treated inGenera Orchidacearum, from which we reconstructed the evolution of epiphytism and other traits including stem and leaf succulence, while testing the correlated evolution between lifestyle and morphological traits. Furthermore, we reconstructed the ancestral geographic ranges to interpret major character changes during the Cenozoic.Key ResultsEpiphytism evolved at least 7.1 My ago in the neotropical Sobralieae, 11.5 My ago in the Arethuseae in Southeast Asia and Australia, and 39.0 My ago in the common ancestor of the Dendrobieae and Cymbidieae in the three previous areas, and was notably lost in the Malaxideae, Collabieae, Calypsoeae, Bletiinae, and Eulophiinae. Stem succulence is inferred to have evolved once, in a terrestrial ancestor 43.1 My ago, thus preceding the evolution of epiphytism by at least 4.1 My. If lost, stem succulence was almost systematically replaced by leaf succulence in epiphytic lineages.ConclusionsEpiphytism probably evolved from terrestrial orchids already possessing succulent stems, which appeared during Eocene climatic cooling. Both epiphytic and secondary terrestrial Epidendroideae may have appeared in seasonally-dry forests. Thus, we believe that the emergence of stem succulence in early epidendroids was a key innovation in the evolution of orchids, facilitating the colonisation of epiphytic environments that led to the greatest diversification of orchids.
A ubiquitous spectrolaminar motif of local field potential power across the primate cortex
Authors: Mendoza-Halliday Diego , Major Alex J. , Lee Noah , Lichtenfeld Maxwell , Carlson Brock , Mitchell Blake , Meng Patrick D. , Xiong Yihan (Sophy) , Westerberg Jacob A. , Maier Alexander , Desimone Robert , Miller Earl K. , Bastos André M. 
Published: October 2022

AbstractThe mammalian cerebral cortex is anatomically organized into a six-layer motif. It is currently unknown whether a corresponding laminar motif of neuronal activity exists across the cortex. Here, we report such a motif in the power of local field potentials (LFP). We implanted multicontact laminar probes in five macaque monkeys and recorded activity across layers in 14 cortical areas at various hierarchical processing stages in all cortical lobes. The anatomical laminar locations of recordings were histologically identified via electrolytic lesions. In all areas, we found a common spectrolaminar pattern characterized by an increasing deep-to-superficial layer gradient of gamma frequency LFP power peaking in layers 2/3, and an increasing superficial-to-deep gradient of alpha-beta power peaking in layers 5/6. Our results show an electrophysiological dissociation between superficial and deep layers that is preserved across the cortex, suggesting a ubiquitous layer and frequency-based mechanism for cortical computation.
The PTEX pore component EXP2 is important for intrahepatic development during the Plasmodium liver stage
Authors: Hussain Tahir , Linera-Gonzalez Jose , Beck John M , Fierro Manuel A , Mair Gunnar R , Smith Ryan C , Beck Josh R 
Published: October 2022

AbstractDuring vertebrate infection, obligate intracellular malaria parasites develop within a parasitophorous vacuole which constitutes the interface between the parasite and its hepatocyte or erythrocyte host cells. To transcend this barrier, Plasmodium spp. utilize a dual-function pore formed by EXP2 for nutrient transport and, in the context of the PTEX translocon, effector protein export across the vacuole membrane. While critical to blood stage survival, less is known about EXP2/PTEX function in the liver stage, although major differences in the export mechanism are indicated by absence of the PTEX unfoldase HSP101 in the intrahepatic vacuole. Here, we employed the glucosamine-activated glmS ribozyme to study the role of EXP2 during Plasmodium berghei liver stage development in hepatoma cells. Insertion of the glmS sequence into the exp2 3’UTR enabled glucosamine-dependent depletion of EXP2 after hepatocyte invasion, allowing separation of EXP2 function during intrahepatic development from a recently reported role in hepatocyte invasion. Post-invasion EXP2 knockdown reduced parasite size and largely abolished expression of the mid to late liver stage marker LISP2. As an orthogonal approach to monitor development, EXP2-glmS parasites and controls were engineered to express nanoluciferase. Activation of glmS after invasion substantially decreased luminescence in hepatoma monolayers and in culture supernatants at later time points corresponding with merosome detachment that marks the culmination of liver stage development. Collectively, our findings extend the utility of the glmS ribozyme to study protein function in the liver stage and reveal EXP2 is important for intrahepatic parasite development, indicating PTEX components also function at the hepatocyte-parasite interface.
Human HelQ and DNA polymerase δ interact to halt DNA synthesis and stimulate DNA single-strand annealing
Authors: He Liu , Lever Rebecca , Cubbon Andrew , Tehseen Muhammad , Jenkins Tabitha , Nottingham Alice O. , Horton Anya , Betts Hannah , Fisher Martin , Hamdan Samir M. , Soultanas Panos , Bolt Edward L. 
Published: October 2022

AbstractDNA strand breaks can be repaired by base-pairing with unbroken homologous DNA, forming a template for new DNA synthesis that patches over the break site. In eukaryotes multiple DNA break repair pathways utilize DNA polymerase δ (Pol δ) to synthesise new DNA from the available 3’OH at the strand break. Here we show that DNA synthesis by human Pol δ is halted by the HelQ DNA repair protein directly targeting isolated Pol δ or Pol δ in complex with PCNA and RPA. The mechanism is independent of DNA binding by HelQ or Pol δ, maps to a region of HelQ that also modulates RPA, and requires multiple Pol δ subunits. Interaction of HelQ with the POLD3 subunit of Pol δ stimulated DNA single-strand annealing activity of HelQ. The data implicates HelQ in preventing genetic instability by restraining DNA synthesis in multiple DNA break repair pathways.
The scaffold proteins Tanc1 and Tanc2 are required for myoblast fusion
Authors: El-Khoury Michelle , Lalonde Andréane , Hipfner David , Côté Jean-François 
Published: October 2022

AbstractMyoblast fusion is a crucial step in myogenesis during embryogenesis and adulthood. In Drosophila, the scaffold protein Antisocial (Ants)/Rols7 plays is essential in myoblast fusion by connecting the cell adhesion surface proteins to the cytoskeleton. Most molecular pathways governing fusion are evolutionary conserved between mammals and flies, but the relative contributions of Tanc1 and Tanc2, mammalian orthologs of Ants/Rols7, in myoblast fusion have not been established. We used the myoblast C2C12 cell line as a model for differentiation and fusion to assess the contributions of Tanc1 and Tanc2 in fusion. We found that Tanc1 and Tanc2 expressions are not modulated during differentiation, but that both proteins are enriched at the cell cortex in proliferating myoblasts. The knockdown of either Tanc1 or Tanc2 in either of the fusing myoblasts impaired fusion. Notably, the expression of human Tanc1 or Tanc2 restored fusion defects observed in Tanc1- or Tanc2-depleted cells. We found that neither Tanc1 nor Tanc2 could substitute for Ants/Rols7 during Drosophila myoblast fusion. We conclude that both Tanc1 and Tanc2 play a role in mammalian myoblast fusion, but there may be some mechanistic differences with the functions of the Drosophila orthologous protein.
Molecular mechanism underlying the increased risk of colorectal cancer metastasis caused by single nucleotide polymorphisms in LI-cadherin gene
Authors: Yui Anna , Kuroda Daisuke , Maruno Takahiro , Nakakido Makoto , Nagatoishi Satoru , Uchiyama Susumu , Tsumoto Kouhei 
Published: October 2022

AbstractLI-cadherin is a member of the cadherin superfamily. LI-cadherin mediates Ca2+-dependent cell-cell adhesion by forming a homodimer. A previous study reported two single nucleotide polymorphisms (SNPs) in the LI-cadherin-coding gene (CDH17). These SNPs correspond to the amino acid changes of Lys115 to Glu and Glu739 to Ala. Patients with colorectal cancer carrying these SNPs are reported to have a higher risk of lymph node metastasis than patients without the SNPs. Although proteins associated with metastasis have been identified, the molecular mechanisms underlying the functions of these proteins remain unclear, making it difficult to develop effective strategies to prevent metastasis. In this study, we employed biochemical assays and molecular dynamics (MD) simulations to elucidate the molecular mechanisms by which the amino acid changes caused by SNPs in the LI-cadherin-coding gene increase the risk of cancer metastasis. Cell aggregation assays showed that the amino acid changes weakened the LI-cadherin-dependent cell-cell adhesion. In vitro assays demonstrated a decrease in homodimerization tendency due to the mutation of Lys115, and MD simulations suggested an alteration in the intramolecular hydrogen bond network due to the amino acid change. Taken together, our results indicate that the increased risk of lymph node metastasis is due to weakened cell-cell adhesion caused by the decrease in homodimerization tendency.