bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hyperaccumulator plant increase selenium absorption by optimizing the microbiota in rhizosphere and regulating root-secreted key exudates
Authors: Huan zhang , Yang Dandan , Hu Chengxiao , Du Xiaoping , Shi Guangyu , Wang Xu , Han Chuang , Yuan Xiang , Li Keyi , Chen Siqi , Zhao Xiaohu 
Published: May 2022

Abstract
Background: Selenium (Se) is a necessary human micronutrient, and around a billion people worldwide may be Se deficient. This can be ameliorated by Se biofortification of staple crops. Plant-associated microbiomes have essential functions in improving plant nutrition. Se hyperaccumulator plant can recruit specific microbes in rhizosphere, however, it was unclear whether rhizosphere microbes and specific microbes can be assembled for application in the promotion of Se uptake by plant, and also the mechanism was incompletely understood.Results: Significant differences were found in rhizosphere microbial community structure between Se hyperaccumulator and non-hyperaccumulator plant. Se contents in root of non-hyperaccumulator plant were increased by 255% with the rhizosphere microbiome from Se hyperaccumulator plant, while 18.3%, 7.6%, 8.9% and 89.3% increase with the key microorganisms of Bacillus Megaterium, Chryseobacterium, Pseudomonas nitritireducens and the synthetic bacterial community, respectively. A preference by specific rhizosphere bacteria for consumption of palmitic acid, stearic acid and glycolic acid, glutamine, 1-monomyristin, aminoethyl alcohol exuded from roots were observed using metabonomic technology by spilt root experiment. Besides, the root development, Se transport-related gene expression of non-hyperaccumulator and available Se in soil were promoted by key functional strains from hyperaccumulator rhizosphere. Notably, the expression amount of BnSULtr1;1, ATPs and BnPt1;4 in root treated by synthetic bacterial community were significantly increased by 344%, 151% and 96.5%, respectively. Furthermore, chemotaxis effect between rhizosphere key bacteria, amino acids as well as organic acids, the role of glycolic acid, glutamine and aminoethyl alcohol in biofilm production, which contributed to key bacteria successfully colonization in root, thus inducing Se promoted uptake by plant.Conclusions: Much was known about the effect of plant-associated microbiomes on promoting Se uptake, but less was understood about whether and how the specific bacteria community of Se hyperaccumulator make effect in this process. Our results demonstrated positive effect and synergistic mechanism that rhizosphere key bacteria in hyperaccumulator plant promote Se uptake by regulating root metabolism, improving Se transport gene expression, promoting root development and increasing the Se availability in soil. This discovery provides a mechanistic underpinning for the process and reference for effective utilization of rhizosphere microbes in Se uptake by plant.
Migrasome Derived from Retinal Pigmented Epithelium Cells Contributes to the Development of Proliferative Vitreoretinopathy
Authors: Wu Liangjing , Yang Shuai , Li Hui , Zhang Yao , Feng Le , Zhang Conghui , Wei Jiayi , Gu Xunyi , Xu Guotong , Wang Zhaoyang , Wang Fang 
Published: May 2022

Abstract
Background：Proliferative vitreoretinopathy (PVR) is a blind-causing disease initiated by the activation of retinal pigmented epithelium (RPE) primarily induced by TGF-β families. Migrasomes is a recently discovered type of extracellular vesicles related to cell migration. Results: Here, we used ex vivo, in vitro, and in vivo models, to investigate the characteristics and functions of migrasomes in RPE activation and PVR development. Results indicated that the migrasome marker tetraspanin-4 (TSPAN4) was abundantly expressed in human PVR-associated clinical samples. The ex vivo model PVR microenvironment is simulated by incubating brown Norway rat RPE eyecups with TGF-β1. Electron microscope images showed the formation of migrasome-like vesicles during the activation of RPE. Further studies indicated TGF-β1 increased the expression of TSPAN4 which results to migrasome production. Migrasomes can be internalized by RPE and increase the migration and proliferation ability of RPE. Moreover, TSPAN4-inhibited RPE cells are with reduced ability of initiating experimental PVR. Mechanically, TSPAN4 expression and migrasome production is induced through TGF-β1/Smad2/3 signaling pathway. Conclusions：In conclusion, migrasomes can be produced by RPE under PVR microenviroment. Migrasomes play a pivotal role in RPE activation and PVR progression. Thus, targeting TSPAN4 or blocking migrasome formation might be a new therapeutic method against PVR.
Evidence of Fluctuating Integrated Stress Response Activity in Murine Primordial Ovarian Follicles
Authors: Hagen-Lillevik Synneva , Rushing John , Cari Evelyn Llerena , Lersten Ivy , Polotsky Alex J. , Lai Kent , Johnson Joshua 
Published: May 2022

Abstract
We are evaluating the action of the Integrated Stress Response (ISR) pathway and how its enforcement of cell cycle arrest and enhancement of a cytoprotective state contribute to the rate of primordial follicle (PF) loss. Physiological stress and damage are variable locally/regionally within the ovary, thus we predict that ISR activity within PFs will be locations-dependent. We therefore tested whether levels of the marker of the active ISR, serine 51-phosphorylation of eukaryotic Initiation Factor 2 alpha subunit (P-eIF2α), differed between oocytes and pregranulosa cells within PFs, between PFs in the same ovary, and between PFs in ovaries collected from different animals. Quantitative fluorescence analysis revealed that P-eIF2α levels correspond tightly between oocytes and pregranulosa cells within the same PFs, but that different follicles in the same ovary have varied levels of the ISR activity marker; this was consistent between animal replicates. The variation of ISR activity in PFs may therefore directly reflect local/regional differences in physiological factors that induce cell damage and stress. This feature of the dynamically changing intra-ovarian environment may influence the likelihood that PFs stay dormant or begin to grow.
Job Satisfaction Prediction and Machine Learning Technique
Authors: Choi Youngkeun , Choi Jae 
Published: May 2022

Abstract
This paper aims to develop a reliable job satisfaction prediction model using machine learning technique. For this, this study used the dataset which is available at IBM Watson Analytics and applied generalized linear model including linear regression and binomial classification. This study essentially had two primary approaches. Firstly, this paper intends to understand the role of variables in job satisfaction prediction modeling better. Secondly, the study seeks to evaluate the predictive performance of generalized linear model including linear regression and binomial classification. In these results, first, we can predict employees’ job satisfaction with a lot of individual factors. Second, for each model, our model showed the outstanding predictive performance. The pre-access and modeling methodology used in this paper can be viewed as a roadmap for the reader to follow the steps taken in this study and to apply procedures to identify the causes of many other human resource management problems.
Urine concentration in elderly women and men
Authors: Hahn Robert G. 
Published: May 2022

Abstract
Background: Concentrated urine reflects high degree of renal fluid conservation, which is of relevance to healthcare. Middle-aged women have less concentrated urine than men. The present observational study evaluated whether this difference remains in elderly hospital patients and in acute medical conditions. Methods: Urine sampling was performed in 1,316 subjects for analysis of urine-specific gravity, osmolality, creatinine, and color. Patients were recruited from three hospital settings (median age, 78 years), while 27% of the subjects were healthy controls (median age, 43 years). Results: Biomarker values were significantly higher in men than in women in all settings, indicating less intense renal water conservation in women. Statistically significant sex differences were found in 354 healthy volunteers, in 286 patients who delivered a urine sample just before surgery, in 311 patients seeking acute care, and in 360 patients who underwent in-hospital geriatric care. The average difference was 6% for urine-specific weight, 14% for osmolality, 25% for creatinine, and 19% for urine color. The biomarker values decreased with age and with the occurrence of albuminuria. Female sex was independently associated with significantly lower values of all four biomarkers, whereas age and albuminuria showed mixed results. More dilute urine was found in patients seeking medical attention for heart conditions, including angina pectoris and heart failure. Conclusion: Women in the hospital setting have less concentrated urine than men even after correction for age and albuminuria.
Blockchain and Ipfs Based Secure Cloud Banking System Using Smart Card
Authors: Biswas Ankur , Roy Abhishek 
Published: May 2022

Abstract
The advancement of Information and Communication Technology (ICT) has made it possible for humans to send messages electronically. Generally, this concept of electronic message transmission is useful for prompt and cost effective mode of communication between the connected users. People are being pushed to maintain social distance during the current Corona virus (COVID19) epidemic in order to break the chain of viral transmission throughout society. This approach of electronic message communication has evolved as the primary mode of communication between the users for availing various types of services like banking, education, administration, healthcare, etc. While focusing on the promptness and cost effective approach of electronic message communication, it is equally important to maintain highest standard of Privacy, Integrity, Non-Repudiation and Authentication (PINA) for digitally transmitted messages, so that neither the end user nor the service provider can be adversely affected by the attacker. Recently in India, various cases of fraudulent monetary transactions has been noticed in the banking sector, which has resulted to miss utilization of hard earned money of Citizen. The occurrence of these fraudulent monetary transactions in the banking sector has explored scope for research work to make the entire system more reliable and accountable to its beneficiaries. Apart from that, because electronic message communication is typically conducted over an open communication medium such as the Internet, which is highly susceptible to adversarial illicit attempts, research work should be conducted to provide the populace with an integrated and secure electronic banking system. Furthermore, it is also essential to simulate the security parameters of electronic banking system before its real world implementation, so as to ensure investment of resources in right direction. To achieve this objective, in this paper authors has proposed Blockchain and Interplanetary File System (IPFS) based secured Cloud Banking System (CBS) and performed its Object Oriented Modelling (OOM) to emulate its deployment.
First report of a sleeper shark (Somniosus sp.) in the western Caribbean, off the insular slope of a coral atoll.
Authors: Kasana Devanshi , Martinez Hector Daniel , Faux Omar , Monzon Neri , Guerra Elio , Chapman Demain D 
Published: May 2022

Abstract
A sleeper shark (Somniosus sp.) was captured over the insular slope at Glover’s Reef, a coral atoll in Belize. The estimated total length of the individual was ca. 3.0-3.5 m and multiple morphological features diagnostic of a somniosid shark were observed. While the exact species could not be confirmed, it is most likely a Greenland shark (Somniosus microcephalus) or a hybrid between the Greenland shark and the Pacific sleeper shark (Somniosus pacificus). This is the first record of a sleeper shark in the western Caribbean region and further supports the hypothesis that these sharks, best known from polar and subpolar latitudes, occur at depth in tropical regions.
The impact of obstructive sleep apnea on growth in patients with syndromic and complex craniosynostosis
Authors: Yang S , Mathijssen I.M.J. , Joosten K.F.M. 
Published: May 2022

Abstract
Purpose: Given the high prevalence of OSA in children with syndromic and complex craniosynostosis (SCC) and the consequences of untreated OSA, it is important to assess their nutritional status and growth. Yet, literature regarding growth in children with SCC remain scarce. Therefore, this study aimed to 1) illustrate the growth pattern in SCC, 2) determine the impact of OSA on this growth pattern, and 3) to evaluate the effect of surgical treatment of OSA on growth over time.Methods: A retrospective study was performed in children with SCC, who are treated at the Dutch Craniofacial Center (Rotterdam, The Netherlands). Growth variables (height, weight, weight-for-age SDS, weight-for-height SDS, and height-for-age SDS) and degree of OSA (obstructive apnea-hypopnea index) were assessed. Results: Of the 153 children with SCC, 38 (25%) were acutely malnourished at some point during follow-up, of whom 21 had disease-related acute malnutrition. Children with moderate-severe OSA had significant lower weight-for-height SDS compared to children without OSA (p = 0.0063). Growth parameters (weight-for-age SDS, weight-for-height SDS, height-for-age SDS) in children with SCC without OSA were not impaired as it did not differ from the normal healthy population, with exception of the patients with Saethre-Chotzen syndrome (SCS) who had a significantly lower SDS for height-for-age.Conclusions: Children with SCC have a substantial chance of developing acute malnutrition at some point during growth. Additionally, in children with moderate-severe OSA a significant lower SDS for weight-for-height is present, indicating the importance of assessing the weight and growth pattern in children who are clinically suspected for OSA.
Rock Brittleness Evaluation Index based on Ultimate Elastic Strain Energy
Authors: Zhang Xiao-Wu , Xu Jin-Hai , Shaikh Faiz , Sun Lei , Cao Yue 
Published: May 2022

Abstract
Brittleness is an essential parameter to determine the deformation and failure behavior of rocks and it is useful to quantify the brittleness of rocks in numerus engineering practices. Taking the relationship between the energy evolution characteristics in the complete stress-strain curve and the brittleness of rocks under external loads into account, a novel energy-based brittleness evaluation index is proposed in this study, which redefined the dissipated proportion of ultimate elastic strain energy relative to post-peak failure energy and residual elastic strain energy. To verify the reliability and accuracy of the novel energy-based brittleness index, a series of conventional triaxial compression (CTC) tests were performed on shale specimens. The calculated results show that the novel energy-based index can precisely reflect the deformation and failure characteristic of rocks under different confining pressures. Meanwhile, the CTC tests data on six types of rocks from previous studies are used to verify the universality of the novel index. The decreasing trend of the brittleness value with increasing confining pressure indicates that the novel brittleness index can characterize various types of rocks and reveal a wide range of rock brittleness. Accordingly, the novel brittleness index proposed in the research can present more kinds of rocks and is more precise to characterize the brittleness of rock than the other existing brittleness indices.
Effect of Short-Term Use of Sevoflurane on QT Interval in Children Undergoing Eye Surgery
Authors: Khademi Saeed , Jouybar Reza , Ghadimi Maryam , Razavi Mahsa , Shahriary Ensiyeh 
Published: May 2022

Abstract
Studies have shown that taking anesthesia drugs can increase QT interval. We investigated the effect of short-term use of sevoflurane on QT and QTc intervals in the early stages of anesthesia and in children. In this prospective cohort study, 52 patients aged 2–14 years, were enrolled. Children were randomly divided into two groups: sevoflurane (S) and control (sodium thiopental, P). In the intervention group, anesthesia induction using sevoflurane was continued for 10–15 minutes and the sevoflurane level was adjusted so that the end-expiratory concentration remained constant at 3%. In group P (control group), anesthesia was induced with 5 mg/kg sodium thiopental. ECGs were taken at 0, 5, 10, and 15 minutes after induction of anesthesia and compared between both groups. QT measurement in lead II in the four times showed that the two groups were not significantly different from each other, and all values obtained were in the normal range and less than 0.44. Measurement of QT in lead V5 showed that the QT interval between groups increased 10 and 15 minutes postoperatively in both groups, and this increase was more in group P than the S group. In group S, QT values in 10 and 15 minutes compared to time 0 and 5 were associated with an increase of about 0.01 seconds and the increase was not significant. Short-term use of sevoflurane does not increase QT and QTc levels in children undergoing surgery without intubation and need for muscle relaxants.
Ovarian mucinous borderline tumor with anaplastic carcinomatous nodules in adolescents
Authors: Huang Mengqi , Lv Qian , Xie Jingyan 
Published: May 2022

Abstract
Bilateral ovarian epithelial neoplasms in adolescents are rare. Moreover, borderline mucinous neoplasms with local intraepithelial carcinoma with anaplastic carcinoma are even more infrequent. Herein, we presented a single case (a 17-year-old female) with regular menstrual cycles and stomach pain when eating who was diagnosed with a left ovarian tumor accompanied by mural nodules. The right ovarian cyst, the left ovary, and the fallopian tube were removed by surgery. Intraoperative diagnosis suggested a bilateral ovarian tumor with mural nodules, which include three different pathological types: sarcomatoid transformation, anaplastic carcinoma, and sarcoma. Paclitaxel combined with carboplatin was given for 6 cycles after an operation, and gonadotropin-releasing hormone agonist (GnRHa) was given at the beginning of chemotherapy for 3 cycles for ovarian function protection. Regular follow-up (the last follow-up was performed 48 months after the operation) of gynecological ultrasound and tumor indicators did not indicate recurrence. In clinical practice, it is necessary to pay attention to the symptoms such as abdominal pain in adolescent females. Routine non-invasive pelvic ultrasound is recommended to fully evaluate the nature of the tumor before surgery, and decide the operation mode. Also, intraoperative frozen pathology of the tissue should be performed as soon as possible.
Sentence Continuation Inference of Urdu Text by BERT Technique
Authors: Ahmed Saad Bin 
Published: May 2022

Abstract
The transformer-based BERT pre-trained language model has helped to improve state-of-the-art performance on a number of natural language processing (NLP) tasks.The BERT for Arabic has been well researched and has shown to be effective.However, the BERT for Urdu has yet to be tested.In this study, we show how pre-trained BERT models may be used to recognise cursive text such as Urdu.We employed pre-trained models because a dataset for synthetic Urdu was not accessible for research, thus we provided a synthetic Urdu dataset of 53k characters and got good results. If we could provide the BERT with a large amount of data, it would deliver impressive results.Initial results for Urdu text show that if we provide more data to the BERT pre-trained model, accuracy will improve even further.
SMO mutation affects tumor immuno-microenvironment and predicts the effect of immune checkpoint inhibitor: from NSCLC to multiple-cancers
Authors: Ji Wenxiang , Niu Xiaomin , Yu Yongfeng , Li Ziming , Gu Linping , Lu Shun 
Published: May 2022

Abstract
A critical and challenging process in immunotherapy is to identify cancer patients who could benefit from immune checkpoint inhibitors (ICIs). Exploration of predictive biomarkers could help to optimize the clinical benefits. SMO mutation was found to be enriched in patients responding to ICIs therapy. In the discovery NSCLC cohort (N = 349), significant differences were detected between SMO_MUT and SMO_WT patients regarding objective response rate (ORR, 53.8% vs. 23.7%, p = 0.021), durable clinical benefit (DCB, 69.2% vs. 35.2%, p = 0.018), and median progression-free survival (PFS, 23.0 m vs. 3.8 m, adjusted p = 0.041), which was further confirmed in the validation NSCLC cohort (PFS, 9.3 m vs. 4.8 m, adjusted p = 0.038). In the pan-cancers cohort (N = 1347), significant overall survival advantage was observed in SMO mutation patients (not reach [NR] vs. 18 m, adjusted p = 0.024). In subgroup analysis, the survival advantage of SMO_MUT vs. SMO_WT was prominent and consistent across gender, age, treatment, cancer type, and TMB (all Pinteraction > 0.05). In further exploring, SMO mutation status was found to be connected with higher tumor mutation burden, more neoantigen load, more DDR mutations, higher MSI score and more CD8+ T cell infiltration. Multiple immune segments including antigen processing and presentation, NK/CD4+/CD8 + T cell and interferon gamma response were found to be activated in SMO_MUT tumors. SMO mutation status was an independent prognostic factor, which can predict better clinical outcomes in ICIs treatment across multiple cancer types.
Association between serum lipids and apolipoproteins level and chronic subdural hematoma: a case-control study
Authors: Che Xudong , Fu Wenqiao , Long Haibo , Yang Xiaolin , Wen Tangmin , Su Jun , He Zhaohui 
Published: May 2022

Abstract
Background: Several studies, including a randomized clinical trial, have demonstrated that atorvastatin may be an effective nonoperative treatment for chronic subdural hematoma (CSDH). Atorvastatin is mainly used to regulate lipid metabolism. However, few studies investigated the association between serum lipids level and risk of CSDH.Methods: We conducted a retrospective case-control study to assess the relationship between fasting lipids (total cholesterol, triglyceride, HDL-C and LDL-C), apolipoproteins (ApoA1, ApoB) and risk of CSDH. Using a one-to-three case–control ratio, 137 CSDH patients were included in our study. Conditional logistic regression was used to examine the relationship between lipids and apolipoproteins biomarkers and risk of CSDH.Result: In univariate analysis, baseline levels of total cholesterol (P<0.001), HDL-C (P<0.001) and ApoA1 were significantly lower among patients with CSDH, while levels of ApoB were higher. No significant difference for triglyceride (P=0.15) and LDL-C (P=0.47) were observed. In multivariable analysis, total cholesterol (OR for the highest vs lowest quartile 0.34, 05%CI 0.19-0.64, P<0,001), triglycerides (OR 1.98, 95%CI 1.05-3.71, p<0,05), HDL-C (OR 0.26, 95%CI 0.13-0.52, P<0,001), ApoA1 (OR 0.27, 95%CI 0.14-0.52, P<0,001) were significantly associated with CSDH, when conditioned on age and gender, and adjusted for head trauma and hypertension. Additional adjustment for all covariates of interest don’t substantially change the multivariable associations.Conclusion: Among a panel of lipids and apolipoproteins biomarkers, total cholesterol, triglycerides, HDL-C, ApoA1 were significantly associated with CSDH.