bluuebunny/crossref_metadata_2025_split huggingface dataset:
A modified Delphi study to identify screening items to assess neglected sexual side-effects following prostate cancer treatment
Authors: Röscher Pierre , Naidoo Kimesh , Milios Joanne E. , van Wyk Jacqueline M. 
Published: March 2022

Abstract
Background
Neglected sexual side effects (NSSE) are a group of less common sexual side effects that may present after Prostate Cancer (PCa) treatment. There is currently no valid and reliable tool to identify these side effects. A modified Delphi study is an effective way of developing the content of such a screening tool.

Methods
A modified Delphi study was used to obtain consensus from a multi-disciplinary group of experts over 3 rounds during a 12 week period. Ten statements were presented containing 8 closed-ended statements on individual NSSEs, and 2 open-ended statements on psychosocial impact related to NSSE. Consensus was defined as a 75% strongly agree achievement on each statement, or the final statement evolution at the end of 3 rounds. Statement support in each round was determined by mean, standard deviation and range, after a numerical value was allocated to each statement during specific rounds. All three rounds were structured and suggestions and additions were incorporated in the statement evolution of the three rounds.

Results
Thirty-five participants were invited, and 27 completed Round 1 (RD 1), 23 participants completed RD2, and 20 participants completed RD3. All 3 rounds were completed in 12 weeks. Statement 1 (sexual arousal incontinence), statement 2 (climacturia) and statement 3 (orgasm intensity) reached consensus after RD2, and statement 9 (sexual dysfunction impact) and statement 10 (experiences) were removed after RD3. Statement 4 (orgasmic pain), statement 5 (anejaculation), statement 6 (sensory disturbances), statement 7 (penile length shortening) and statement 8 (penile curvature) were finalised after the conclusion of RD3. Statements 1–3 were the most stable statements with the most support and least amount of disagreement. Statements 4–8 were less stable, but support for them improved over the 3 rounds. Statements 9–10 both had good stability, but the support indicated that they needed to be removed from the set of statements. Statement 5 had the poorest range due to an outlier opinion.

Conclusions
Consensus was reached on the items making up the NSSE screening tool. Health care practitioners will be able to use this tool to identify the evidence of NSSE after PCa treatment. Further testing will be undertaken to confirm the reliability and validly of the tool.
Morphometric study of the ventricular indexes in healthy ovine BRAIN using MRI
Authors: Trovatelli Marco , Spediacci Carlotta , Castellano Antonella , Bernardini Andrea , Dini Daniele , Malfassi Luca , Pieri Valentina , Falini Andrea , Ravasio Giuliano , Riva Marco , Bello Lorenzo , Brizzola Stefano , Zani Davide Danilo 
Published: March 2022

Abstract
Background
Sheep (Ovis aries) have been largely used as animal models in a multitude of specialties in biomedical research. The similarity to human brain anatomy in terms of brain size, skull features, and gyrification index, gives to ovine as a large animal model a better translational value than small animal models in neuroscience. Despite this evidence and the availability of advanced imaging techniques, morphometric brain studies are lacking. We herein present the morphometric ovine brain indexes and anatomical measures developed by two observers in a double-blinded study and validated via an intra- and inter-observer analysis.

Results
For this retrospective study, T1-weighted Magnetic Resonance Imaging (MRI) scans were performed at 1.5 T on 15 sheep, under general anaesthesia. The animals were female Ovis aries, in the age of 18-24 months. Two observers assessed the scans, twice time each. The statistical analysis of intra-observer and inter-observer agreement was obtained via the Bland-Altman plot and Spearman rank correlation test.
The results are as follows (mean ± Standard deviation):
Indexes: Bifrontal 0,338 ± 0,032 cm; Bicaudate 0,080 ± 0,012 cm; Evans’ 0,218 ± 0,035 cm; Ventricular 0,241 ± 0,039 cm; Huckman 1693 ± 0,174 cm; Cella Media 0,096 ± 0,037 cm; Third ventricle ratio 0,040 ± 0,007 cm.
Anatomical measures: Fourth ventricle length 0,295 ± 0,073 cm; Fourth ventricle width 0,344 ± 0,074 cm; Left lateral ventricle 4175 ± 0,275 cm; Right lateral ventricle 4182 ± 0,269 cm; Frontal horn length 1795 ± 0,303 cm; Interventricular foramen left 1794 ± 0,301 cm; Interventricular foramen right 1,78 ± 0,317 cm.

Conclusions
The present study provides baseline values of linear indexes of the ventricles in the ovine models. The acquisition of these data contributes to filling the knowledge void on important anatomical and morphological features of the sheep brain.
HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway
Authors: Guo Kai , Ma Zhiqiang , Zhang Yujiao , Han Lu , Shao Changjian , Feng Yingtong , Gao Fei , Di Shouyin , Zhang Zhipei , Zhang Jiao , Tabbò Fabrizio , Ekman Simon , Suda Kenichi , Cappuzzo Federico , Han Jing , Li Xiaofei , Yan Xiaolong 
Published: March 2022

Abstract
Background
Histone deacetylases (HDACs) play crucial roles in cancers, but the role and mechanism of HDAC7 in NSCLC have not been fully understood.

Methods
A total of 319 patients with non-small cell lung cancer (NSCLC) who underwent surgery were enrolled in this study. Immunohistochemistry and Kaplan–Meier survival analysis were performed to investigate the relationship between HDAC7, fibroblast growth factor 18 (FGF18) expression, and clinicopathologic characteristics. Cell functional experiments were implemented both in vivo and in vitro to investigate the effects on NSCLC cell proliferation and metastasis. Recombinant lentivirus–meditated in vivo gene overexpression or knockdown, real-time polymerase chain reaction (PCR), western blotting, and coimmunoprecipitation assays were applied to clarify the underlying molecular mechanism of HDAC7 in promoting NSCLC progression.

Results
The elevated expression of HDAC7 or FGF18 was positively correlated with poor prognosis, tumor–node–metastasis (TNM) stage, and tumor differentiation of NSCLC patients. NSCLC patients with co-expressed HDAC7 and FGF18 suffered the worst prognosis. HDAC7 overexpression promoted NSCLC proliferation and metastasis by upregulating FGF18. Conversely, overexpression of FGF18 reversed the attenuated ability in tumor growth and metastasis mediated by downregulating HDAC7. In terms of mechanism, our results suggested that the interaction of HDAC7 with β-catenin caused decreased β-catenin acetylation level at Lys49 and decreased phosphorylation level at Ser45. As a consequence, the HDAC7-mediated posttranslational modification of β-catenin facilitated nuclear transfer and activated FGF18 expression via binding to TCF4. Furthermore, deubiquitinase USP10 interacted with and stabilized HDAC7. The suppression of USP10 significantly accelerated the degradation of HDAC7 and weakened NSCLC growth and migration.

Conclusions
Our findings reveal that HDAC7 promotes NSCLC progression through being stabilized by USP10 and activating the β-catenin-FGF18 pathway. Targeting this novel pathway may be a promising strategy for further developments in NSCLC therapy.
Moxidectin: heartworm disease prevention in dogs in the face of emerging macrocyclic lactone resistance
Authors: Savadelis Molly D. , McTier Tom L. , Kryda Kristina , Maeder Steven J. , Woods Debra J. 
Published: March 2022

AbstractHeartworm (Dirofilaria immitis) disease continues to increase and spread, remaining one of the most important and pathogenic parasitic diseases of dogs, despite the regular use of macrocyclic lactones (MLs) in preventive products. Dogs harboring strains ofD. immitisresistant to MLs, the only drug class available for heartworm prevention in the United States, have been documented and proven. As no new products are available utilizing a novel drug class for the prevention of this disease, the only options for combating ML resistance include increasing the dose and/or changing the dosage regime of current MLs, or by optimizing the formulations of MLs currently available. Moxidectin provides a unique opportunity for optimization of the dose and formulation, which may provide improved efficacy against ML-resistant strains. Currently there are oral, topical, and injectable moxidectin products approved for heartworm prevention in the USA. Two new products (ProHeart®12 and Simparica Trio®), available in many countries around the world including the USA, take advantage of the unique attributes of moxidectin for providing robust heartworm prevention against the strains of heartworm to which most dogs in the USA will likely be exposed. Both products have demonstrated 100% preventive efficacy in laboratory studies against recently collected field strains of heartworm, and also in large field studies, where the majority of dogs were living in the southern USA in areas where ML resistance has been confirmed to occur, therefore under elevated heartworm challenge. Based on the data summarized here, these products offer important advances in heartworm prevention and provide additional options for veterinarians and pet owners to protect their dogs from developing heartworm disease.Graphical Abstract
Adoption of ICT innovations in the agriculture sector in Africa: a review of the literature
Authors: Ayim Claudia , Kassahun Ayalew , Addison Chris , Tekinerdogan Bedir 
Published: March 2022

AbstractAccording to the latest World Economic Forum report, about 70% of the African population depends on agriculture for their livelihood. This makes agriculture a critical sector within the African continent. Nonetheless, agricultural productivity is low and food insecurity is still a challenge. This has in recent years led to several initiatives in using ICT to improve agriculture productivity. However, a systematic review of the evidence categorized by the various aspects of the topic is lacking. This study investigates the state of the art of ICT innovations within the agriculture sector in Africa. To achieve this, we reviewed the literature published from 2010 to 2019 in which ICT innovations were discussed. Our search in four major literature databases yielded 779 papers, of which we selected 23 primary studies for a detailed analysis. The analysis shows that the main ICT technologies adopted are text and voice-based services targeting mobile phones. The analysis also shows that radios are still widely used in disseminating agriculture information to rural farmers, while computers are mainly used by researchers. Though the mobile-based services were aimed at improving access to accurate and timely agriculture information, the literature review indicates that the adoption of the services is constrained by poor technological infrastructure, inappropriate ICT policies, and low level of user skills, especially of farmers, in using the technologies.
Genomic patterns of homozygosity and inbreeding depression in Murciano-Granadina goats
Authors: Luigi-Sierra María Gracia , Fernández Almudena , Martínez Amparo , Guan Dailu , Delgado Juan Vicente , Álvarez Javier Fernández , Landi Vincenzo , Such Francesc Xavier , Jordana Jordi , Saura María , Amills Marcel 
Published: March 2022

Abstract
Background
Inbreeding depression can adversely affect traits related to fitness, reproduction and productive performance. Although current research suggests that inbreeding levels are generally low in most goat breeds, the impact of inbreeding depression on phenotypes of economic interest has only been investigated in a few studies based on genealogical data.

Results
We genotyped 1040 goats with the Goat SNP50 BeadChip. This information was used to estimate different molecular inbreeding coefficients and characterise runs of homozygosity and homozygosity patterns. We detected 38 genomic regions with increased homozygosity as well as 8 ROH hotspots mapping to chromosomes 1, 2, 4, 6, 14, 16 and 17. Eight hundred seventeen goats with available records for dairy traits were analysed to evaluate the potential consequences of inbreeding depression on milk phenotypes. Four regions on chromosomes 8 and 25 were significantly associated with inbreeding depression for the natural logarithm of the somatic cell count. Notably, these regions contain several genes related with immunity, such as SYK, IL27, CCL19 and CCL21. Moreover, one region on chromosome 2 was significantly associated with inbreeding depression for milk yield.

Conclusions
Although genomic inbreeding levels are low in Murciano-Granadina goats, significant evidence of inbreeding depression for the logarithm of the somatic cell count, a phenotype closely associated with udder health and milk yield, have been detected in this population. Minimising inbreeding would be expected to augment economic gain by increasing milk yield and reducing the incidence of mastitis, which is one of the main causes of dairy goat culling.
An exploration of cultural influencing factors on dietary diversity in Malagasy children aged 6–59 months
Authors: Ribeli Jacqueline , Pfister Franziska 
Published: March 2022

AbstractBackgroundMore than 1.7 million children in Madagascar are stunted, with low dietary diversity being a determinant. Although diverse crops are produced in the Vakinankaratra region, it registers the highest stunting prevalence rate nationally. While children’s diets may be influenced by region-specific cultural factors, little is known about this link so far. The aim of this study was to determine the influence of cultural habits on dietary diversity in children between 6 to 59 months in the Vakinankaratra region.MethodsA mixed method design with a qualitative lead approach was used, including three transect walks, six focus group discussions and 51 semi-structured interviews with caregivers. The interviews contained a quantitative part to assess the four feeding indicators: ‘minimum meal frequency’, ‘minimum dietary diversity’, ‘minimum acceptable diet’ and the consumption of iron-rich foods. Participants were selected by convenience sampling. Data was collected in November 2019 in three communities displaying maximal possible variation.ResultsSubsistence farming with diverse crops and livestock was found to be a common practice. Minimum meal frequency was achieved by 78% of the sixty included children. In total, 45% attained minimum dietary diversity and 40% consumed a minimum acceptable diet. Across the three communities, the range of children attaining a minimum acceptable diet was 25–60%. Older, no longer breastfed children were prone to not achieving a minimum acceptable diet. Although caregivers had a basic idea of important foods for child development, these were often not available to or affordable for them. An effect of cultural events on diet, especially diversity, was found. Whilst for annual events this effect was short-term, the region-specific ceremony of reburying the dead (Famadihana) was found to have a long-term influence on the diet of certain families.ConclusionsThe dietary diversity of children under five in the Vakinankaratra region is influenced by cultural factors like agricultural practices, caregivers’ knowledge of child nutrition, food taboos and a region-specific cultural event. Cultural determinants, especially important traditions that result in significant expenditures, may thus influence the quality of children’s diet and should therefore be considered in future nutrition programmes and research on child malnutrition and stunting.
Impact of containment measures on community mobility, daily confirmed cases, and mortality in the third wave of COVID-19 epidemic in Myanmar
Authors: Htun Ye Minn , Win Tun Tun , Shan Nyan Htet , Winn Zin Thu , Thu Kaung Si , Maung Nyan Lin , Aung Pyae Phyo , Kyaw Htun Aung , Kyaw Hpone Pji , Soe Yan Naing Myint , Ko Myint Myat , Aung Zin Ko , Aung Kyaw Thiha , Lwin Yan Paing Chit , Yan Wai , Soe Phyo Tayza , Tun Kyaw Myo 
Published: March 2022

AbstractIn Myanmar, third wave of COVID-19 epidemic began with a surge of confirmed cases in the last week of May 2021. The laboratory-confirmed cases and deaths distinctly increased within 9 weeks. The government and the Ministry of Health adopted containment measures to flatten the peak of the epidemic and to suppress the disease transmission. The strictly containment measures: stay-at-home restrictions, school closure, and office closure have reduced the community mobility, confirmed cases and mortality. Therefore, the timely containment measures implemented by the government were important to reduce the transmission as observed in the third wave of COVID-19 epidemic in Myanmar.
Flipping the flipped class: using online collaboration to enhance EFL students’ oral learning skills
Authors: Fischer Ian Douglas , Yang Jie Chi 
Published: March 2022

AbstractExtensive research on the in-class synchronous learning aspect of the flipped classroom approach (FCA) has rendered favorable results the past decade. However, less attention has been placed on the out-of-class component of the FCA, specifically the drawbacks that may occur, such as low student accountability while watching the pre-assigned video lessons, thus coming to class unprepared, and not using the latest online technological to its full potential. This study addresses these issues by examining the impact of creating a more synchronous/collaborative online out-of-class flipped-class component. Using the latest online technology, a newly proposed flipped group (PFG) was created and then compared to a regular flipped group (RFG), and a traditional class (TC) over 14 weeks. Fifty-four undergraduate business students from a university in Taiwan participated in the study. Mock International English Language Testing System (IELTS) oral pre/post-tests were given to all participants to investigate which teaching approach is more effective. The results showed the PFG significantly improved overall on average, out-preforming the RFG. Surprisingly, the TC significantly performed better than the RFG. In addition, significant differences and correlations also occurred between the PFG and RFG students’ online learning behaviors and objective performances, such as the time spent online viewing the assigned video lessons and the effects on their quiz and final grade scores. This study’s findings support creating a more synchronous/collaborative online learning environment can enhance the out-of-class component of a FCA, and therefore help improve student’s overall oral/aural EFL learning.
Prevalence and risk factors for suicide in patients with sepsis: nationwide cohort study in South Korea
Authors: Oh Tak Kyu , Park Hye Yoon , Song In-Ae 
Published: March 2022

BackgroundAlthough a recent study reported that survivors of critical illness have an increased risk of suicide, the suicide rate and factors associated with suicide in patients with sepsis have not yet been investigated.AimsWe aimed to examine the prevalence and risk factors of suicide among patients with sepsis in South Korea.MethodAll adult patients who were admitted to all hospitals in South Korea with a main diagnosis of sepsis, from 1 January 2010 to 31 December 2018, were included in the study. The primary outcome was suicide within 1 year after sepsis diagnosis.ResultsA total of 251 837 adult patients with sepsis were included, of which 132 691 patients (52.7%) died within 1 year after the diagnosis of sepsis, and death by suicide was the cause in 3903 patients (1.5%). Older age, male gender, living in a rural area, higher Charlson Comorbidity Index and Elixhauser Comorbidity Index scores, invasive treatment (continuous renal replacement therapy and mechanical ventilator support) and admission to a hospital with low annual case volumes were associated with a higher risk of suicide. In addition, concurrent substance misuse, post-traumatic stress disorder, bipolar disorder, dementia and previous attempt of suicide or self-harm were associated with a higher risk of suicide.ConclusionsDuring the 1-year follow-up period, 1.5% of patients died by suicide after the diagnosis of sepsis in South Korea. Knowledge of the factors associated with suicide might allow for earlier intervention to potentially reduce the number of suicide attempts in patients with sepsis.
Mental health consequences of COVID-19 in house staff physicians
Authors: Espinosa-Guerra Edward , Rodríguez-Barría Edgar , Donnelly Christl , Carrera Jean-Paul 
Published: March 2022

Background: A new coronavirus, SARS-CoV-2, was associated with a newly identified respiratory syndrome, COVID-19 in Wuhan, China, in early December 2019. SARS-CoV-2 rapidly spread across the globe, imposing increased working hours and workloads for healthcare workers. We have evaluated the prevalence of mental health outcomes and associated factors in house staff physicians in Panama. Methods: A cross-sectional study was undertaken from July 23, 2020, to August 13, 2020. Snowball sampling was used to recruit participants. Then, an electronic questionnaire with scales to evaluate anxiety disorders (GAD-7), depression (PHQ-9) and post-traumatic stress (IES-R) was administered. In addition, socio-demographic variables, clinical history of mental disorders and COVID-19 exposure were evaluated. Independent analyses for each mental health outcome were undertaken using a logistic regression analysis. Results: A total of 517/1,205 (42.9%) interns and residents were recruited nationwide. The overall prevalence of depression symptoms was 25.3%, 13.7% for anxiety and 12.2% for post-traumatic stress. At least 9.3% participants reported having suicidal ideation. The most parsimonious model showed females had a higher prevalence of mental health disorders across results, and married participants were more likely to present depression (OR, 1.73; 95% CI, 1.03-2.91; P = 0.039) or at least one mental health disorder (OR, 1.66; 95% CI, 1.03-2.68; P = 0.039). Conclusions: A high prevalence of mental health disorders was found, showing the need to mitigate this emotional burden among healthcare workers in the current context of the COVID-19 pandemic.
Synthesis and characterization of silica xerogel from corn husk waste as cationic dyes adsorbent
Authors: Dahliyanti Ayu , Yunitama Devi Aryanihanan , Rofiqoh Iftahul Maulina , Mustapha Mazli 
Published: March 2022

Background: High corn production and consumption in Indonesia have led to massive corn husk waste. To increase the economic value of corn husk waste, innovations have utilized its silica content as an alternative source for the synthesis of multifunctional silica compounds. Methods: In this research, silica xerogel was successfully synthesized from corn husk via the sol-gel method. Its physical properties and capabilities as an adsorbent to remove cationic dyes such as crystal violet and methylene blue in wastewater were investigated for the first time. Results: The as-synthesized silica xerogel possesses an amorphous crystal structure with an average pore diameter of 6.35 nm, a Brunauer, Emmett, and Teller (BET) surface area of 363.72 m2/g, and particle size ranging from 80 to 250 μm. The optimum contact times of silica xerogel are 90 minutes for crystal violet and 120 minutes for methylene blue adsorptions. While at the same time, the dye removal efficiencies are as high as 98.539% and 96.184%, respectively. The adsorption of both crystal violet and methylene blue on silica xerogel follows the Langmuir isotherm model and exhibits a high monolayer capacity of 69.44 mg/g and 59.17 mg/g, respectively. Conclusions: These findings suggest the great potential of silica xerogel synthesized from corn husk as a low-cost and effective cationic dye adsorbent.