bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative transcriptomic analysis of the malaria parasites Plasmodium falciparum and Plasmodium vivax sensitive and resistant strains
Authors: Sakpal S. , Abdeen S. Z. , Kothari Sh. L. , Bastikar V. 
Published: February 2022

Introduction. Malaria is the sixth leading cause of death worldwide. According to a WHO survey (2019-2020), the total number of malaria deaths is estimated to be 409000. Plasmodium falciparum and Plasmodium vivax are major malaria parasites, particularly in subtropical areas.Materials and methods. In the present study, we used a transcriptome analysis of raw RNA sequence data to identify and characterize the differentially expressed genes in Plasmodium falciparum chloroquine-sensitive and chloroquine-resistant strains, as well as Plasmodium vivax primaquine sensitive and primaquine resistant strains. The raw RNA sequence data were obtained from the NCBI SRA database using the Accession IDs PRJNA308455, SRR14191963, and SRR332573.Results and discussion. The sequence of raw RNA was quantified, mapped, and annotated. The total number of reads mapped to the reference genome for Plasmodium falciparum was found to be 45474448 and for Plasmodium vivax was 38226870. The Cufflinks-Cuffdiff tool was used to identify differentially expressed genes in Plasmodium falciparum and Plasmodium vivax sensitive and resistant strains. This differentially expressed gene was further annotated and plotted using the "Limma" package of Bioconductor. The PPI network was constructed in String Database and Cytoscape software. Pathway enrichment analysis of list differentially expressed gene performed using KEGG and GO tool.Conclusion. In sensitive and resistant strains, comparative transcriptome analysis revealed differentially regulated gene expression patterns.
Development of Celecoxib Granules for Manufacturing of Prolonged Release Celecoxib Capsules and Tablets
Authors: Alkhodri A. , Suslina S. N. 
Published: February 2022

Introduction. To reduce frequency of taking celecoxib dosage forms, as a highly effective drugs of the latest generation for the treatment of inflammatory diseases of the musculoskeletal system, it is advisable to develop pharmaceutical dosage form with modified release, which will reduce side effects and costs treatment.Aim. The purpose of this study was to develop formulations of prolonged release celecoxib granules.Materials and methods. Experiments were carried out with materials and equipments of the scientific laboratories of the company LEM pharma (Hama, Syria). The physico-chemical and technological properties of the experimental samples of granules and tablets of celecoxib were determined according to the methods of the American Pharmacopoeia USP 41 NF 36 edition.Results and discussion. The choice of excipients, in particular, cellulosic polymers to control releasing celecoxib was carried out. The compositions of six model samples of celecoxib granules were proposed. The technological characteristics of the samples of granules (index Hausner, Carr ratio, Particle size distribution and moisture content) have been determined, using a dissolution test, their suitability for obtaining capsules and matrix tablets of prolonged release has been established.Conclusion. The compositions of granules celecoxib with modified release are developed, which can be used to obtain capsules, as well as matrix tablets as the final pharmaceutical form.
Development of Effervescent Granules with Solid Dispersion of Furazolidone
Authors: Elagina A. O. , Beliatskaya A. V. , Krasnyuk (Jr.) I. I. , Krasnyuk I. I. , Stepanova O. I. , Vorob'yov A. N. , Fateeva T. V. 
Published: February 2022

Introduction. Treatment of infectious and inflammatory diseases demands active substances (AS), characterized by low resistance of microorganisms, high specificity of the mechanism of action and a wide range of antimicrobial activity. Though furazolidone (FZ) – AS meets these criteria, however, practically insolubility in water significantly limits its use. Use of the solid dispersion method (SD) could increase the solubility and dissolution rate of AS with low water solubility. revious studies indicate an increase in the solubility and dissolution rate of FZ in water from SD with polyvinylpyrrolidone-24 000 (PVP-24 000) in a ratio with AS > 6 : 1 by weight. As a result, it becomes possible to introduce SD FZ into the composition of rapidly dissolving effervescent dosage forms, for example, granules for obtaining solutions for external use for the treatment of infectious and inflammatory diseases.Aim. Development of the composition and technology for producing effervescent granules based on solid dispersions of FZ for obtaining a solution for external use.Materials and methods. FZ substance, polyvinylpyrrolidone-24 000 ± 2000 (PVP-24 000 ± 2000), tartaric acid, malic acid, sodium carbonate anhydrous, ethyl alcohol 96 %, purified water. We provided the granules through fluidized bed granulation. The analysis of the obtained granules included the following parameters: description, granule size, loss in mass on drying, disintegration, uniformity of dosage according to GPМ 1.4.1.0004.15 "Granules". Then followed a qualitative and quantitative determination of AS and analysis of the pH of the aqueous solution of the granules. To study the stability and shelf life of the samples of granules, we laid them to the storage in accordance with GPМ 1.10009.15 "Stability and shelf life of drugs".Results and discussion. We developed he composition and technology of FZ effervescent granules for obtaining a solution for external use. Granules are obtained by separate granulation of the main (containing a SD AS) and acid components, followed by mixing in ratios that provide a solution of FZ with a concentration of 0.004 % in water at room temperature. Later we assessed the quality of the obtained compositions and determined, the shelf life (2 years) and storage conditions (in a dry, dark place at a temperature of 25 °C) of the developed compositions of granules.Conclusion. As a result of technological and chemical-pharmaceutical research using the SD method, we developed a new FZ dosage form – effervescent granules, which makes it possible to obtain an aqueous solution with a concentration of FZ 0.004 % in less than 5 minutes without heating. Based on the results of the work, an application was filed with Rospatent No. 2021105988 dated 10.03.2021, "Fast-dissolving dosage form of furazolidone and a method for its production".
Determination of Optimal Technological Conditions for the Process of Production a Liposomal Dosage Form of Cyphetrylin
Authors: Dmitrieva M. V. , Orlova O. L. , Lantsova A. V. , Krasnyuk I. I. 
Published: February 2022

Introduction. The Laboratory for the development of dosage forms of the N. N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of Russian Federation has developed a composition of an injectable liposomal dosage form based on soybean phosphatidylcholine, a hydrophobic analog of the hypothalamic hormone somatostatin − cyphetrylin, intended for the treatment of neuroendocrine tumors.Aim. Determination of optimal technological conditions for the process of obtaining a liposomal dosage form of cyphetrylin.Materials and methods. The study used the substance cyphetrylin synthesized in the of Chemical Synthesis Laboratory of the N. N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of Russian Federation; soybean phosphatidylcholine S PC and polyethylene glycol-2000-distearoylphosphatidylethanolamine, produced by Lipoid GmbH (Germany); cholesterol ≥99 % (Sigma-Aldrich, Japan). For the preparation of phospholipid vesicles loaded with cyphetrylin and "empty", the methods of lipid film hydration with subsequent extrusion or homogenization of the dispersion of multi-layer liposomes were used. The obtained liposomal samples were evaluated by quality indicators − the effectiveness of the inclusion of cyphetrylin in vesicles, their average size and surface charge (zeta potential), the viscosity of the dispersion. To evaluate these characteristics, the methods of spectrophotometry, laser scattering spectroscopy, determination of the electrophoretic mobility of particles and viscometry were used.Results and discussion. It was found that the most suitable organic solvent for obtaining a solution of cyphetrylin and lipid components of the dosage form is chloroform. The duration of the drying period of the lipid film under vacuum to remove the residual solvent is determined by the ratio of the volume of the distillation flask used and the mass of its loading its loading. At the same time, hydration off the lipid film with water for injection should be carried out under conditions of low-pressure and room temperature. To obtain a dispersion of single-layer cyphetrylin liposomes of the required size, an extrusion method using nylon and polycarbonate membranes with a filter pore diameter of 0.22 and 0.2 um, respectively, was chosen.Conclusion. The optimal technological conditions for the process of obtaining a liposomal dosage form in the form of a dispersion of a hydrophobic analog of the hypothalamic hormone somatostatin − cyphetrylin are determined.
Adaptación y normativización del Inventario del Desarrollo Comunicativo Mac Arthur Bates (CDI-Forma II) al español rioplatense
Authors: Resches Mariela - , Querejeta Maira , Kohan Cortada Ana , Laguens Ana 
Published: February 2022

En este trabajo se presenta la adaptación al español rioplatense de la Forma II de los Inventarios del Desarrollo Comunicativo Mac Arthur-Bates (CDI), destinado a evaluar el vocabulario expresivo y las habilidades morfosintácticas entre los 16 y los 30 meses. Se expone, en primer lugar, el procedimiento de adaptación del instrumento. En segundo lugar, se presentan algunas evidencias de su fiabilidad y validez, al tiempo que se describen las principales tendencias evolutivas encontradas tras la aplicación del inventario a una muestra de 726 madres y/o padres de niños y niñas de las edades arriba mencionadas. Los resultados indican, en primer lugar, que la versión rioplatense del CDI presenta elevados niveles de consistencia interna y de representatividad de sus ítems léxicos. En segundo lugar, las trayectorias del desarrollo léxico y gramatical observadas se han mostrado sensibles a los cambios en relación con la edad, de modo comparable al de otras adaptaciones del CDI. Al mismo tiempo, se han encontrado correlaciones moderadas y significativas entre el aumento del vocabulario y la complejidad morfosintáctica, aún después de controlar el efecto de la edad. Por último, se ha observado un efecto significativo del nivel educativo materno sobre el tamaño del léxico expresivo y la longitud media de las primeras frases. Se concluye que la versión rioplatense del CDI representa una contribución necesaria y promisoria para la evaluación del lenguaje temprano en el contexto sudamericano.
Effects of Plant Extracts on Dentin Bonding Strength: A Systematic Review and Meta-Analysis
Authors: Zhao Shikai , Hua Fang , Yan Jiarong , Yang Hongye , Huang Cui 
Published: February 2022

Objective: To systematically review in vitro studies that evaluated the effects of plant extracts on dentin bonding strength.Materials and Methods: Six electronic databases (PubMed, Embase, VIP, CNKI, Wanfang and The Cochrane Library) were searched from inception to September 2021 in accordance with the Preferred Reporting Items for Systematic Reviews (PRISMA). In vitro studies that compared the performance of dental adhesives with and without the plant extracts participation were included. The reference lists of the included studies were manually searched. Two researchers carried out study screening, data extraction and risk of bias assessment, independently and in duplicate. Meta-analysis was conducted using Review Manager 5.3.Results: A total of 62 studies were selected for full-text analysis. 25 articles used the plant extracts as primers, while five added the plant extracts into adhesives. The meta-analysis included 14 articles of in vitro studies investigating the effects of different plant extract primers on dentin bonding strength of etch-and-rinse and self-etch adhesives, respectively. The global analysis showed statistically significant difference between dental adhesives with and without plant extract primers. It showed that the immediate bond strength of dental adhesives was improved with the application of plant extract primers.Conclusion: The application of proanthocyanidin (PA) primers have positive effect on the in vitro immediate bonding strength of dental adhesives irrespective of etch-and-rinse or self-etch modes.
Predictors and Outcomes of Healthcare-Associated Infections Caused by Carbapenem-Nonsusceptible Enterobacterales: A Parallel Matched Case-Control Study
Authors: Hoo Grace S. R. , Cai Yiying , Quek Yan Ching , Teo Jocelyn Q. , Choudhury Saugata , Koh Tse Hsien , Lim Tze Peng , Marimuthu Kalisvar , Ng Oon Tek , Kwa Andrea L. 
Published: February 2022

ObjectivesThe increasing incidence of carbapenem-nonsusceptible Enterobacterales as major pathogens in healthcare associated infections (HAIs) is of paramount concern. To implement effective prevention strategies against carbapenem-nonsusceptible Enterobacterales (CnSE) HAIs, it is crucial to identify modifiable factors associated with these infections. We identified risk factors for CnSE-HAIs, and compared clinical outcomes of CnSE-HAI and carbapenem-sensitive Enterobacterales (CSE)-HAI patients.MethodsWe conducted a multi-centre parallel matched case-control study in two 1700-bedded Singapore acute-care hospitals from 2014–2016. Patients with CnSE-HAIs and CSE-HAIs were compared to a common control group without HAIs (1:1:3 ratio), matched by time-at-risk and patient ward. Carbapenem nonsusceptible was defined as non-susceptibility to either meropenem or imipenem. Presence of healthcare associated infections were defined by the criteria provided by the European Centre for Disease Prevention and Control. Outcomes of CnSE-HAI and CSE-HAI patients were compared using multivariable logistic and cox regression; the models were adjusted for infection and treatment characteristics.ResultsEighty CnSE-HAI and 80 CSE-HAI patients were matched to 240 patients without HAIs. All CRE-HAIs patients had prior antibiotic exposure, with 44 (55.0%) with prior carbapenem exposure. The most common CnSE-HAIs were intra-abdominal infections (28.8%) and pneumonia (23.8%). The most common CnSE species was Klebsiella spp. (63.8%). In the risk factor analysis, presence of drainage devices [adjusted odds ratio (aOR), 2.19; 95% CI, 1.29 – 3.70] and prior carbapenem exposure (aOR,17.09; 95% CI, 3.06 – 95.43) independently predicted CnSE-HAIs. In the crude outcomes analysis, CnSE-HAI patients had higher all-cause in-hospital mortality and longer time to discharge compared to CSE-HAI patients. After adjusting for differences in receipt of antibiotics with reported susceptibility to the Enterobacterales, there was no significant difference in all-cause in-hospital mortality between the two groups (aOR, 1.76; 95% CI, 0.86–3.58). Time to discharge remained significantly longer in patients with CnSE-HAI (adjusted hazard ratio, 0.71; 95% CI, 0.51 – 0.98) after adjusting for disease severity, receipt of antibiotics with reported susceptibility and receipt of appropriate source control.ConclusionAppropriate management of deep-seated Enterobacterales infections and reducing exposure to carbapenems may reduce risk of CnSE-HAIs in Singapore. Efforts to improve antimicrobial therapy in CnSE-HAI patients may improve patient outcomes.
Survivals of Angiography-Guided Percutaneous Coronary Intervention and Proportion of Intracoronary Imaging at Population Level: The Imaging Paradox
Authors: Ng Andrew Kei-Yan , Ng Pauline Yeung , Ip April , Lam Lap-Tin , Siu Chung-Wah 
Published: February 2022

BackgroundThere is a significant disparity between randomized controlled trials and observational studies with respect to any mortality benefit with intracoronary imaging during the percutaneous coronary intervention (PCI). This raises a suspicion that the imaging paradox, in which some operators may become over reliant on imaging and less proficient with angiography-guided PCI, might exist.MethodThis was a retrospective cohort study from 14 hospitals under the Hospital Authority of Hong Kong between January 1, 2010 and December 31, 2017. Participants were patients who underwent first-ever PCI. The association between mortality risks of patients undergoing angiography-guided PCI and three tertiles (low, medium, and high) of the proportion of PCI done under intracoronary imaging guidance at a population level (background imaging rate), were evaluated after confounder adjustment by multivariable logistic regression.ResultsIn an adjusted analysis of 11,816 patients undergoing angiography-guided PCI, the risks of all-cause mortality for those were higher in the high-tertile group compared with the low-tertile group (OR, 1.45, 95% CI, 1.10–1.92, P = 0.008), the risks of cardiovascular mortality were higher in the high-tertile group compared with the low-tertile group (OR, 1.51, 95% CI, 1.08–2.13, P = 0.017). The results were consistent with multiple sensitivity analyses. Threshold analysis suggested that the mortality risks of angiography-guided PCI were increased when the proportion of imaging-guided PCI exceeded approximately 50%.ConclusionsThe risks of the all-cause mortality and cardiovascular mortality were higher for patients undergoing angiography-guided PCI in practices with a higher background imaging rate.
Associations of Renalase With Blood Pressure and Hypertension in Chinese Adults
Authors: Wang Yang , Chen Chen , Hu Gui-Lin , Chu Chao , Zhang Xiao-Yu , Du Ming-Fei , Zou Ting , Zhou Qing , Liao Yue-Yuan , Ma Qiong , Wang Ke-Ke , Sun Yue , Wang Dan , Yan Yu , Li Yan , Jia Hao , Niu Ze-Jiaxin , Zhang Xi , Wang Lan , Man Zi-Yue , Gao Wei-Hua , Li Chun-Hua , Zhang Jie , Gao Ke , Li Hui-Xian , Chang John , Desir Gary V. , Lu Wan-Hong , Mu Jian-Jun 
Published: February 2022

ObjectiveRenalase, a novel secretory flavoprotein with amine oxidase activity, is secreted into the blood by the kidneys and is hypothesized to participate in blood pressure (BP) regulation. We investigated the associations of renalase with BP and the risk of hypertension by examining renalase single nucleopeptide polymorphism (SNPs), serum renalase levels, and renal expression of renalase in humans.Methods① Subjects (n = 514) from the original Baoji Salt-Sensitive Study cohort were genotyped to investigate the association of renalase SNPs with longitudinal BP changes and the risk of hypertension during 14 years of follow-up. ② Two thousand three hundred and ninety two participants from the Hanzhong Adolescent Hypertension Study cohort were used to examine the association of serum renalase levels with hypertension. Renalase expression in renal biopsy specimens from 193 patients were measured by immunohistochemistry. ③ Renalase expression was compared in hypertensive vs. normotensive patients.Results① SNP rs7922058 was associated with 14-year change in systolic BP, and rs10887800, rs796945, rs1935582, rs2296545, and rs2576178 were significantly associated with 14-year change in diastolic BP while rs1935582 and rs2576178 were associated with mean arterial pressure change over 14 years. In addition, SNPs rs796945, rs1935582, and rs2576178 were significantly associated with hypertension incidence. Gene-based analysis found that renalase gene was significantly associated with hypertension incidence over 14-year follow-up after adjustment for multiple measurements. ② Hypertensive subjects had higher serum renalase levels than normotensive subjects (27.2 ± 0.4 vs. 25.1 ± 0.2 μg/mL). Serum renalase levels and BPs showed a linear correlation. In addition, serum renalase was significantly associated with the risk of hypertension [OR = 1.018 (1.006–1.030)]. ③ The expression of renalase in human renal biopsy specimens significantly decreased in hypertensive patients compared to non-hypertensive patients (0.030 ± 0.001 vs. 0.038 ± 0.004).ConclusionsThese findings indicate that renalase may play an important role in BP progression and development of hypertension.
Alcohol Consumption and Risk of Atrial Fibrillation: A Dose-Response Meta-Analysis of Prospective Studies
Authors: Zhang Heng-Zhi , Shao Bo , Wang Qi-Yu , Wang Yi-Han , Cao Ze-Zhong , Chen Lu-Lu , Sun Jin-Yu , Gu Mu-Feng 
Published: February 2022

BackgroundThis study aimed to investigate the dose-response association between alcohol consumption and atrial fibrillation (AF) risk.MethodsPubMed, Embase, Cochrane Library, and Web of Science were systematically searched using keywords related to alcohol and AF from the establishment of databases up to 1 March 2021. Prospective studies examining the impact of alcohol on the risk of AF with hazard ratios (HRs) were included. Restricted cubic spline regression was performed to quantify the dose-response relationship between alcohol consumption and AF risk.ResultsThirteen eligible studies were included in the meta-analysis, with a total of 645,826 participants and 23,079 cases of AF. When compared with non-/seldom-drinkers, the pooled adjusted HRs of AF were 1.30 (95% confidence interval [CI]: 1.20–1.41) and 1.00 (95% CI: 0.96–1.05) for high and low alcohol consumption, respectively. Moderate alcohol intake significantly increased the risk of AF in males (HR, 1.21; 95% CI: 1.10–1.33) but not in females (HR, 1.02; 95% CI: 0.91–1.14). The cubic spline regression analysis illustrated that the risk of AF significantly increased with daily alcohol intake in a Non-linear manner (R2 = 0.64, P = 5.785 × 10−12).ConclusionThis study revealed a Non-linearly positive association between alcohol intake and the risk of AF. Low alcohol intake was not associated with the development of AF, whereas moderate alcohol intake significantly increased the risk of AF in males but not in females. Our meta-analysis highlighted that alcohol consumption should be restricted to a low level to reduce the risk of AF.