bluuebunny/crossref_metadata_2025_split huggingface dataset:
An N-glycoproteomics Analysis Reveals Glycoprotein Alterations in Esophageal Squamous Cell Carcinoma
Authors: Gao Yingzhen , Shen Liuyi , Dong Tianyue , Yang Xin , Cui Heyang , Guo Yanlin , Ma Yanchun , Kong Pengzhou , Cheng Xiaolong , Zhang Ling , Cui Yongping 
Published: January 2022

Abstract
Background: Aberrant glycosylation has been recognized as a hallmark of cancer. It usually includes the changes of expression level and function of glycoproteins caused by abnormal glycosylation modification and changes of glycan structure of glycoproteins. N-glycosylation is one of the main types of glycosylation in all eukaryotes.Methods: We integrated global proteomics and N-glycoproteomics analysis of ten cases to reveal the N-glycoproteomics profiles in esophageal squamous cell carcinoma (ESCC) compared with normal tissues. Results: A series of differentially expressed glycoproteins (e.g. LAMP2, PLOD2) and some differentially glycoproteins enriched signaling pathway (e.g. metabolism-related pathway, ECM-receptor interaction, focal adhesion) were identified in ESCC. Seven significantly enriched motifs were found from all of the identified N-glycosylation sites. The dynamic profiling changes of glycoprotein during lymph node metastasis progression in ESCC was also investigated and three clusters showed the obvious increasing or decreasing trend with lymph node metastasis.Conclusion: In summary, we presented the characters of N-glycosylation and N-glycoprotein alterations associated with ESCC. Specific changes in the expression of glycoproteins or glycosylation occupancy have the potential to be used as the biomarker to discriminate ESCC from normal tissues and to improve our understanding of ESCC biology.
Neurotransmitter Activation Sequesters Specific mRNAs Into Stress Granules
Authors: Jackson Lela , Poussaint Allison , Scarlata Suzanne 
Published: January 2022

Abstract
Hormones and neurotransmitters can activate the Gαq / phospholipase Cβ1 (PLCβ1) signaling system eliciting cellular calcium responses. Aside from the plasma membrane, PLCβ1 has a cytosolic population that binds stress granule associated proteins preventing their aggregation, and activation of Gαq shifts PLCβ1 to the membrane releasing bound proteins and promoting the formation of stress granules. The cellular impact of stress granules formed upon routine Gαq protein signaling is unknown. Here, we have characterized Ago2 stress granules formed in response to neurotransmitter activation in cultured PC12 cells. We find these stress granules have a distinct protein composition, and unlike stress granules formed upon heat shock, contain only two mRNA transcripts, chromogranin B, which is involved in secretory function, and ATP synthase 5f1b, which is required for ATP synthesis. Our studies show an unexpected pathway where Gαq/PLCβ regulates the translation of specific proteins.
Analysis of Risk Factors Related to Acute Kidney Injury After Liver Transplantation in Children
Authors: Dong Kun , Liu Jianyun , Zhang Cheng , Chen Junze , Dong Chunqiang 
Published: January 2022

Abstract
Objective: To identify risk factors related to the development of acute kidney injury (AKI) in children who underwent liver transplantation.Methods: We retrospectively analyzed data of pediatric patients who underwent liver transplantation at the pediatric surgery department of the First Affiliated Hospital of Guangxi Medical University from July 2017 to November 2018. Subjects were grouped into the non-AKI group (group A) and AKI group (group B). General information, preoperative, intraoperative, and postoperative clinical data were statistically analyzed in the two groups.Results: Among the 31 patients identified, there were 17 cases in group A (six male, 11 female) and 14 cases in group B (seven male and seven female). The baseline data of the patients included age, BMI index; BSA index; PELD score, TBiL count, ALB count, INR index, Scr count, DBiL, and other liver function indexes. There was no difference observed in the demographic and preoperative factors, including infection rates (P >.05) between the two groups. There was no difference between the two groups in terms of intraoperative indicators including vena cava occlusion time, cold ischemia time, warm ischemia time, blood loss, donor liver graft-to-recipient body weight, graft volume/standard liver volume, or use of vasopressor drugs (P>.05). There was no significant difference in postoperative factors (FK506 blood concentration, ICU length of stay, and length of hospital stay) (P>.05). operation of group A (653±205 min) was significantly shorter than that of group B (852±299 min) (P=0.03). The duration of anhepatic phase was shorter in group A (57 ± 15 min) than in group B (73 ± 20 min) (P = 0.02).Conclusion: The incidence of AKI in children after liver transplantation was 45.2%. The operation time and duration of the anhepatic phase were closely related to the occurrence of AKI after liver transplantation in children. Improving the surgical technique and the optimization of surgical procedures can shorten the operation time and the anhepatic phase, therefore reducing the incidence of AKI.
Comparative Studies of Cultural Characteristics of Rhizospheric and Non-rhizospheric Trichoderma spp. On Different Cultural Media
Authors: M CHAITHRA , Pankaja N S 
Published: January 2022

Abstract
Effect of different culture media was observed on rhizospheric and non-rhizospheric seven Trichoderma isolates. All the seven Trichoderma isolates were evaluated on seven different solid media for assessing growth and sporulation. Among the seven different solid media, corn meal agar media and oat meal agar media exhibited early sporulation and maximum mycelia growth rate of more number of Trichoderma isolates. Highest mycelia growth (90 mm) and early sporulation 48 hour after inoculation on maximum number of different solid media was observed by non rhizospheric isolates viz., PSV (Paddy straw) and GMV (Goat manure) over commercial isolate respectively than rhizospheric Trichoderma isolate (Rkd-Cu). So, apart from rhizospheric source we can use non rhizospheric source for isolation of effective Trichoderma isolates which we can future used as good biocontrol agents against different plant pathogens.
A Fast Extraction-Free Isothermal LAMP Assay for Detection of SARS-CoV-2 in Resource-Limited Settings
Authors: Gärtner Kathleen , Meleke Harry , Kamdolozi Mercy , Chaima David , Samikwa Lyson , Paynter Mary , Nyang'Wa Maggie Nyirenda , Cloutman-Green Elaine , Nastouli Eleni , Klein Nigel , Nyirenda Tonney , Msefula Chisomo , Alber Dagmar G. 
Published: January 2022

Abstract
Background

To retain the spread of SARS-CoV-2, fast, sensitive and cost-effective testing is essential, particularly in resource limited settings (RLS). Current standard nucleic acid-based RT-PCR assays, although highly sensitive and specific, require transportation of samples to specialised laboratories, trained staff and expensive reagents. The latter are often not readily available in low- and middle-income countries and this may significantly impact on the successful disease management in these settings. Various studies have suggested a SARS-CoV-2 loop mediated isothermal amplification (LAMP) assay as an alternative method to RT-PCR.
Methods

Four previously published primer pairs were used for detection of SARS-CoV-2 in the LAMP assay. To determine optimal conditions, different temperatures, sample input and incubation times were tested. Ninety-two extracted RNA samples from St. George's Hospital, London, 10 non-extracted nasopharyngeal swab samples from Great Ormond Street Hospital for Children, London, and 92 non-extracted samples from Queen Elisabeth Central Hospital (QECH), Malawi, which have previously been tested for SARS-Cov-2 by qRT-PCR, were analysed in the LAMP assay.
Results

In this study we report the optimisation of an extraction-free colourimetric SARS-CoV-2 LAMP assay and demonstrated that a lower limit of detection between 10-100 copies/µL of SARS-CoV-2 could be readily detected by a colour change of the reaction within as little as 30min. We further show that this assay could be quickly established in Malawi, as no expensive equipment is necessary. We tested 92 clinical samples from QECH and showed the sensitivity and specificity of the assay to be 98.4% and 86.7%, respectively. Some viral transport media, used routinely to stabilise RNA in clinical samples during transportation, caused a non-specific colour-change in the LAMP reaction and therefore we suggest collecting samples in phosphate buffered saline (which did not affect the colour) as the assay allows immediate sample analysis on-site.
Conclusion

SARS-CoV-2 LAMP is a cheap and reliable assay that can be readily employed in RLS to improve disease monitoring and management.
Inspection of Radiation Shielding Proficiency and Effect of Gamma-Ray on ESR, Thermal, and FTIR Properties of Modified Borate Bioglasses by CuO
Authors: Madbouly Amal Mosleh , Abdelghany A.M. , Diab H. M. , Ezz-ElDin F. M. 
Published: January 2022

Abstract
This research studied the various properties of copper oxide (CuO)-doped bioactive borate glasses extensively for their applications in different fields. The radiation shielding properties, such as gamma-ray, neutron, electron, and proton radiation shielding, were investigated. Further, the glasses’ mass attenuation coefficients were measured with a NaI(Tl) detector; their derivative gamma shielding parameters were studied theoretically with the Phy-X/PSD program. Every increase in the CuO % increased the fast neutron (Σ R ) gamma attenuation and effective removal cross-sections. Additionally, mass stopping power (MSP) and projected range (PR) for protons and electrons delivered good results in the 10 KeV‒10 MeV kinetic energy range, as computed by the SRIM code.
Also, the free-radical densities were measured via electron spin resonance to estimate the absorbed doses during accidental irradiation. The extensive reduction of the dose detection threshold (2 Gy) required the estimation of the signal of the non-irradiated sample. This work also studied the effects of applied microwave power incidence and absorbed dose on Cu-containing borate bioglasses. Finally, the thermal annealing of the emerging peaks, which were due to the irradiation signal-to-noise ratio and energy dependence, was studied to estimate the stabilities of such peaks. This modified material is recommended to detect and monitor the gamma-radiation dose because of its good dosimetric properties. Finally, regarding the presence of the two borate groups, triangular (BO 3 ) and the tetrahedrally coordinated (BO 4 ), in their definite and typical wavenumbers, the FTIR spectra displayed simplified vibrations that were close to those of many bioglasses. This paper provides complementary results for the authors previous research examined this glass for low photon dose measurements using luminescence characteristics
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Association of sleep quality and sleep duration with estimated Glomerular filtration rate（eGFR）in a secondary analysis based on a cross-sectional study
Authors: Cao Wei , Cen Ruixiang , Chen Yandan 
Published: January 2022

Abstract
Background: Poor sleep conditions can cause many diseases. However, proof regarding the link of sleep status with renal function in general population is limited. This study was undertaken to investigate the association of sleep quality and sleep duration with estimated Glomerular filtration rate（eGFR）in Taiwanese participants. Methods: The present study was a cross-sectional study. 5714 Taiwanese who visited a health center for a self-motivated general health assessment were collected in this study. The main outcome variables included Pittsburgh Sleep Quality Index（PSQI）and Self-reported sleep duration. The Independent variable is estimated glomerular filtration rate (eGFR). Multivariate linear regression model and two-piecewise linear model are employed to examine the association between outcomes and independent variables after adjusting for covariates involved in this study.Results: After adjusting potential confounders, a non-linear relationship was detected between sleep quality and eGFR, which cut-point was 71. The effect sizes and the 95% confidence intervals on the right sides of inflection point were 0.982(0.967, 0.998) and1.006 (1.001, 1.010).Conclusion: The relationship between sleep quality and eGFR is non-linear. Sleep quality was negatively related to eGFR when eGFR is higher than 71points.
Non-Thermal Argon Plasma Assisted Antibiotic Therapy: Improving the Efficacy of Ciprofloxacin Against Multi-Drug Resistant P. Aeruginosa Biofilm
Authors: Khosravi S. , Jafari S. , Zamani H. , Nilkar M. 
Published: January 2022

Abstract
Bacterial biofilm formation is regarded as the major cause of infection development, therapeutic failure, and antibiotic resistance. For the first time, the current work aims to characterize the synergistic antibiofilm potential of the atmospheric pressure non-thermal argon plasma and Ciprofloxacin (CIP) against multi-drug resistant Pseudomonas aeruginosa. Bacterial biofilms pre-treated with the non-thermal Ar-plasma, and subsequently treated with CIP antibiotic. Then, bacterial biofilm formation level and cell viability were investigated using the crystal violet staining, MTT, SEM, and fluorescence microscopy. It was found that short-time (a few seconds) pretreating with Ar-plasma not only reduced bacterial biofilm but also significantly decreased the viability of the cells within the biofilm matrix. According to the results, treating with CIP alone reduced bacterial biofilm by 32%, while pretreatment with Ar-plasma for 5, 10, 30, 60, and 90 sec and subsequent antibiotic treatment reduced bacterial biofilm by 76, 79, 81, 75, and 88%, respectively. Besides, the viability of bacterial cells within biofilm matrix reduced to 66% in CIP treated cells, while, pretreating with Ar-plasma for 5, 10, 30, 60, and 90 sec significantly decreased cell viability to 28, 25, 21, 23, and 15%, respectively. This concept opens a promising approach for the treatment of drug-resistant bacteria for decontamination of the heat-sensitive materials and human tissues based on the combination of atmospheric pressure non-thermal Ar-plasma and antibiotics.
Elevated remnant cholesterol is closely associated with incident hypertension: a cohort-based study from China
Authors: Wang Guang , Wang Jie , Wang Ying , An Yu , Liu Jia , Leng Song 
Published: January 2022

Abstract
Background Remnant cholesterol (RC) has been confirmed to be associated with cardiovascular events. However, the association between RC and hypertension (HTN) has not been illustrated. Thus, this study aimed to investigate the association of RC with HTN and to compare the predictive values of RC, lipid parameters for the risk of incident HTN in a Chinese population. MethodsThis cohort study included 18483 participants, who are followed for the incidence of HTN. RC were divided into five groups: the < 20% group, the 20–39% group, the 40–59% group, the 60–79% group and the ≥ 80% group, according to quintile division of the participants. Cox proportional hazards models were used to evaluate the association between RC vs other lipid parameters and HTN.ResultsDuring a mean follow-up of 1.1 years, 525 (17.55%) HTN occurred. Multivariate Cox regression analysis shows that compared with conventional lipid parameters, RC remains significantly associated with HTN (hazard ratio (HR) 1.68, 95%confidence interval (CI) 1.40-2.01, p<0.0001). In a stratified analysis, RC is still significant predictive of developing HTN in the subgroups of young age (<55 years) (HR 1.74, 95%CI 1.40-2.16, p<0.0001), as well as with body mass index (BMI) <18.5 kg/m2(HR 2.87, 95%CI 1.30-6.37, p=0.0464) or BMI ≥ 28 kg/m2(HR 1.81, 95%CI 1.11-2.96, p=0.0179), and with fatty liver diseases (HR 1.66, 95%CI 1.17-2.36, p=0.0047).ConclusionsElevated RC is significantly associated with HTN and is a superior predictor for developing HTN independent of other risk factors, suggesting RC may be a new target of managing HTN.
Determinants of Gender Inequality In Sexual Debut Among Youth In Uganda: A Decomposition Analysis
Authors: Mary Luwedde , Sserwanja Quraish , Nehemiah Katantazi 
Published: January 2022

Abstract
Introduction: Teenage pregnancies and sexually transmitted diseases are major public health problems in Uganda. Early sexual debut is one of the main routes for these public health problems. Determinants of gender inequality in sexual debut are crucial for developing gender specific sexual and reproductive health promotion programs that delay sexual debut. Hence the aim of this study was to identify factors that explain gender inequality in sexual debut among Ugandan youth. Methods: This study used secondary data from a cross sectional Uganda demographic health survey of 2016. Participants were 10,235 sexually experienced youth. Using stata 14, intermediary analysis was done to assess statistical association between explanatory variables and sexual debut in a multiple logistic regression analysis. Oaxaca decomposition was used to decompose factors that explain inequalities in sexual debut between male and female youth. Results: Intermediary results showed Islam, many household members, eastern Uganda and being divorced/widowed were predictors of early sexual debut. While secondary education, higher education, blue collar jobs, being 20 to 24 and 25 to 30 years old were protective factors against early sexual debut. Material, behaviour/cultural, psychosocial, and demographic explanatory factors jointly explained a statistically significant portion of the observed gap in sexual debut between female and male youth. More female were at a disadvantage of early sexual debut as compared to male youth. About 96.37% of this gap was explained by unequal distribution of material, behaviour/cultural, psychosocial, and demographic factors between male and female youth. Relationship to household head (49%), education (16.87%), occupation (8,94%), number of household members (8.57%), frequency of using internet (7.99%) and reading newspaper or magazines (4.39%) made significant contribution to the explanation of sexual debut inequality between male and female youth.Conclusions: Results showed sexual debut inequality between female and male youth that favoured men. Programs designed to address early sexual debut and related health outcomes such as teenage pregnancies, HIV must combat inequities in education, sex education, employment opportunities, access of internet and reading newspaper or magazines across gender. They should also foster household relationships and monitor girls to reduce sexual debut inequality across gender.
Preoperative serum CA125 level and age at diagnosis: An effective prognosis prediction tool for patients with early-stage endometrial cancer
Authors: Sun Shuai , Zou Lijuan , Wang Tiejun , Liu Zi , He Jianli , Sun Xiaoge , Zhong Wei , Zhao Fengju , Li Xiaomei , Li Sha , Zhu Hong , Ma Zhanshu , Wang Wenhui , Zhang Fuquan , Hou Xiaorong , Wei Lichun , Hu Ke 
Published: January 2022

Abstract
Objective
To investigate the combined predictive value of the preoperative serum cancer antigen 125 (CA125) level and age at diagnosis among patients with early-stage endometrial cancer(EC) after initial treatment.
Methods
We retrospectively analyzed data from patients with early-stage EC from January 1999 to December 2015 in multiple institutions in China. All patients received primary hysterectomy± bilateral salpingo-oopho rectomy and adjuvant radiotherapy for pathology-confirmed stage I and II EC(FIGO 2009 staging). In total, 447 patients with complete data on preoperative serum CA125 levels were included. All patients were divided into low-risk, intermediate-risk, high-intermediate-risk and high-risk groups according to the ESMO-ESGO-ESTRO risk classification. Binary logistic regression was used to calculate the predictive probability of 5-year overall survival (OS) for each patient, and the receiver operating characteristic (ROC) curve was used to calculate the sensitivity and specificity of variables, as well as the combined predictive value of CA125 and age.
Results
The median follow-up time was 59 months (3-201 months). The 5-year OS and disease-free survival (DFS) rates were 94.4% and 89.1%, respectively. Multivariate analysis showed that the preoperative CA125 level and age at diagnosis were independent prognostic factors for 5-year OS. The best cutoff value forage at diagnosis was 61 years. The cutoff values of CA125 for patients aged ≤61 years and > 61 years were 37.7 U/mL and 39.35 U/mL, respectively. Patients aged >61years with CA125 > 39.35 U/ml and patients aged ≤61years with CA125 > 37.7 U/ml were classified as theCA125-positive group; the remaining patients constituted the CA125-negative group. The area under the curve (AUC) for CA125 combined with age at diagnosis was 0.692, and the corresponding sensitivity and specificity were 68.2% and 68.2% (P<0.002), respectively, which were significantly better than the corresponding values for CA125 or age alone.
Conclusions
Although preoperative CA125 had limited sensitivity in predicting the prognosis for early-stage EC after initial treatment, it remains a useful serum marker for risk assessment of early-stage EC. Combining CA125 with age may increase its predictive sensitivity.
Thrombospondin-2 acts as a bridge between tumor extracellular matrix and immune infiltration in pancreatic adenocarcinoma and stomach adenocarcinoma: An integrative pan-cancer analysis
Authors: Tan Shiyun , Liao Xingchen , Wang Wei , Yu Baoping 
Published: January 2022

Abstract
BackgroundThrombospondin-2 (THBS2) is a versatile glycoprotein that regulates numerous biological functions, including the apoptosis-proliferation balance in endothelial cells, and it has been linked to tumor angiogenesis. However, the role of THBS2 in human cancer remains unknown. This study aimed to determine THBS2 expression in pan-cancer, understand whether THBS2 is associated with its prognosis, and to identify possible roles of THBS2 in tumor immunity and the extracellular matrix (ECM).MethodsData about THBS2 expression in cancers and normal tissues were downloaded from the Genotype-Tissue Expression (GTEx) and UCSC Xena and analyzed using the ONCOMINE database, Perl programming language, and the Gene Expression Profiling and Interactive Analyses vision 2 (GEPIA2) web server. Survival prognosis was analyzed using the survival, survminer, and forestplot packages in R v. 4.0.3 and the limma package in R . Immune and matrix components were also analyzed using R v. 4.0.3. Most importantly, we partially validated the role and mechanism of THBS2 in pancreatic and gastric cancers in vitro using PANC1 and BGC-823 cell lines.ResultsThrombospondin-2 was significantly overexpressed in 17 of the 33 investigated cancers, and linked to a poor prognosis in pan-cancer survival analysis, especially in adrenocortical (ACC), kidney renal papillary cell (KIRP), and stomach (STAD) carcinomas, kidney chromophobe (KICH), and pancreatic adenocarcinoma (PAAD). Immune infiltration and THBS2 expression were also related. The expression of THBS2 has been linked to immune scores, stromal scores, and immune checkpoint markers in various cancers. The PPI network revealed that THBS2 is associated with multiple extracellular matrix proteins and immune proteins. Knockdown of THBS2 decreased the expression of CD47 and MMP-2 as well as the proliferation, migration, and invasion of PANC1 and BGC-823 cells in vitro. ConclusionsOur findings suggested that THBS2 could be a prognostic biomarker that acts as a bridge between the ECM and immune infiltration in various types of cancers, and that it promoted the progression of numerous types of cancers, especially PAAD and STAD.