bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profiling of the ADP‐Ribosylome in Living Cells
Authors: Lehner Maike , Rieth Sonja , Höllmüller Eva , Spliesgar Daniel , Mertes Bastian , Stengel Florian , Marx Andreas 
Published: February 2022

AbstractPost‐translational modification (PTM) with ADP‐ribose and poly(ADP‐ribose) using nicotinamide adenine dinucleotide (NAD+) as substrate is involved in the regulation of numerous cellular pathways in eukaryotes, notably the response to DNA damage caused by cellular stress. Nevertheless, due to intrinsic properties of NAD+ e.g., high polarity and associated poor cell passage, these PTMs are difficult to characterize in cells. Here, two new NAD+ derivatives are presented, which carry either a fluorophore or an affinity tag and, in combination with developed methods for mild cell delivery, allow studies in living human cells. We show that this approach allows not only the imaging of ADP‐ribosylation in living cells but also the proteome‐wide analysis of cellular adaptation by protein ADP‐ribosylation as a consequence of environmental changes such as H2O2‐induced oxidative stress or the effect of the approved anti‐cancer drug olaparib. Our results therefore pave the way for further functional and clinical studies of the ADP‐ribosylated proteome in living cells in health and disease.
Acridine‐Functionalized Covalent Organic Frameworks (COFs) as Photocatalysts for Metallaphotocatalytic C−N Cross‐Coupling
Authors: Traxler Michael , Gisbertz Sebastian , Pachfule Pradip , Schmidt Johannes , Roeser Jérôme , Reischauer Susanne , Rabeah Jabor , Pieber Bartholomäus , Thomas Arne 
Published: February 2022

AbstractCovalent organic frameworks (COFs) are structurally tuneable, porous and crystalline polymers constructed through the covalent attachment of small organic building blocks as elementary units. Using the myriad of such building blocks, a broad spectrum of functionalities has been applied for COF syntheses for broad applications, including heterogeneous catalysis. Herein, we report the synthesis of a new family of porous and crystalline COFs using a novel acridine linker and benzene‐1,3,5‐tricarbaldehyde derivatives bearing a variable number of hydroxy groups. With the broad absorption in the visible light region, the COFs were applied as photocatalysts in metallaphotocatalytic C−N cross‐coupling. The fully β‐ketoenamine linked COF showed the highest activity, due to the increased charge separation upon irradiation. The COF showed good to excellent yields for several aryl bromides, good recyclability and even catalyzed the organic transformation in presence of green light as energy source.
Capabilities and limitations of electrical resistivity tomography for mapping and surveying hillfort fortifications
Authors: Klanica Radek , Křivánek Roman , Grison Hana , Tábořík Petr , Šteffl Jindřich 
Published: February 2022

AbstractHillforts are fortified archaeological sites built from the Neolithic to Early Middle Ages within the area of Europe. They were usually surrounded by fortifications consisting of various combinations of ramparts and ditches, which today constitute their most striking remains. Although magnetometry surveys are commonly used for spatial identification of ramparts and ditches, a different method must be employed for directly obtaining depth information. Hence, we evaluate the potential of electrical resistivity tomography (ERT) for surveying hillfort fortifications. Within three hillforts of different ages in the Czech Republic, we investigated various features affecting the imaging ability of ERT, including bedrock type, building material, present‐day condition of fortification, impact of past or recent agricultural activities, and field settings of the ERT method. Supported by additional information from magnetometry and electromagnetic surveys, the results show that ERT is most applicable in cases of stony ramparts, ditches carved into rocky bedrock or well‐preserved earthen ramparts. Poorer results were achieved upon active and/or recently active agricultural lands, where fortifications have been gradually destroyed by ploughing. The remains of stony ramparts remained distinguishable in the latter case, but mere traces of earthen ramparts and ditches were invisible to ERT due to mixing of fortification material with on‐site soil. ERT is a unique method for detailed investigation of both ramparts and ditches by which a structure and its extent can be evaluated to indicate the function of a settlement and obtain information about former environmental conditions, population, land use and/or human–environmental interaction.
Professional noticing of coordinated mathematical thinking
Authors: Campbell Tye G. , Yeo Sheunghyun 
Published: February 2022

AbstractOver the past three decades, research and policy in many geographic regions has promoted a shift from direct, lecture‐oriented mathematics instruction to inquiry‐based, dialogic forms of instruction. While theory and research support dialogic instructional approaches, some have noted that the complexities of dialogic teaching make it difficult for teachers to implement. One mechanism by which teachers can improve their decision‐making practices in dialogic classrooms is learning to notice (i.e. becoming aware of learners’ processes). While research has contributed frameworks for understanding how teachers notice individual learners’ mathematical thinking, there is little conceptualization regarding how teachers notice group processes in mathematics classrooms, which is integral to dialogic instruction. We offer a noticing framework termed professional noticing of coordinated mathematical thinking that describes how teachers notice group activity in mathematics classrooms. Professional noticing of coordinated mathematical thinking is conceptualized as a bi‐dimensional process: noticing groups’ mathematical activity and noticing groups’ coordinated activity. Teachers must become aware of how groups approach the mathematical and collaborative nature of a task, since both of these aspects inform whether learners develop opportunities to learn in groups. The framework describes noticing practices integral to dialogic instruction and promotes inquiry for future research related to teaching moves in dialogic classrooms.
Model selection characteristics when using MCP‐Mod for dose–response gene expression data
Authors: Duda Julia C. , Kappenberg Franziska , Rahnenführer Jörg 
Published: February 2022

AbstractWe extend the scope of application for MCP‐Mod (Multiple Comparison Procedure and Modeling) to in vitro gene expression data and assess its characteristics regarding model selection for concentration gene expression curves. Precisely, we apply MCP‐Mod on single genes of a high‐dimensional gene expression data set, where human embryonic stem cells were exposed to eight concentration levels of the compound valproic acid (VPA). As candidate models we consider the sigmoid (four‐parameter log‐logistic), linear, quadratic, , exponential, and beta model. Through simulations we investigate the impact of omitting one or more models from the candidate model set to uncover possibly superfluous models and to evaluate the precision and recall rates of selected models. Each model is selected according to Akaike information criterion (AIC) for a considerable number of genes. For less noisy cases the popular sigmoid model is frequently selected. For more noisy data, often simpler models like the linear model are selected, but mostly without relevant performance advantage compared to the second best model. Also, the commonly used standard model has an unexpected low performance.
Development, validation and application of a UHPLC–MS/MS method for quantification of the adiponectin‐derived active peptide ADP355 in rat plasma
Authors: Li Qiaoxi , Jiang Fulin , Guan Yanping , Jiang Xianxing , Wu Junyan , Huang Min , Zhong Guoping 
Published: February 2022

AbstractA UHPLC–MS/MS method for the quantification of ADP355, an adiponectin‐derived active peptide, was developed and validated. The extraction method employed simple protein precipitation using methanol and chromatographic separation was achieved on anAccucore™ RP‐MS C18 column (100 × 2.1 mm, 2.6 μm, 80 Å), using 0.1% formic acid in both water and acetonitrile with gradient elution at the flow rate of 400 μl/min within 4.0 min. Detections were performed under positive ion mode with multiple reaction monitoring ion transitions m/z 1109.2 → 309.8 and 871.4 → 310.1 for ADP355 and Jt003 respectively at unit resolution. The linearity range of the calibration curve was 2–1,000 ng/ml with a lower limit detection of 0.5 ng/ml. The selectivity, linearity, precision, accuracy, recovery, matrix effect and stability were validated, and all items met the requirement of US Food and Drug Administration guidance. This method was successfully applied to an intravenous pharmacokinetic study of ADP355 in rats and the in‐vitro stability in rat serum, plasma and whole blood was also assessed.
Rectosigmoid endometriosis: Diagnostic pitfalls and management – A case report
Authors: Piachas Athanasios , Smyrnis Panagiotis , Tooulias Andreas 
Published: February 2022

AbstractEndometriosis constitutes a benign condition, occurring in 10%–12% of menstruating women. Bowel involvement is estimated to occur in 5%–12% with the rectosigmoid region involved in up to 90% of these cases. We present the case of a 45‐year‐old Caucasian female patient with rectosigmoid endometriosis.
Case series on patients with primary central nervous system lymphoma: From clinical presentations to outcomes
Authors: Anvari Kazem , Welsh James S. , Molaie Fatemeh 
Published: February 2022

AbstractPrimary central nervous system lymphoma (PCNSL) is a rare form of non‐Hodgkin lymphoma. In this report, we present 11 cases of PCNSL which were treated with high‐dose MTX and WBI with a localized radiation boost to the tumor bed.
Cryptococcal infection with ruxolitinib in primary myelofibrosis: A case report and literature review
Authors: Ciochetto Zachary , Wainaina Njeri , Graham Mary Beth , Corey Anna , Abid Muhammad Bilal 
Published: February 2022

AbstractCryptococcus neoformans (CN) is an encapsulated yeast that is found worldwide. It causes self‐limiting infections in immunocompetent hosts; however, infections due to CN could be disseminated and potentially life‐threatening in immunocompromised hosts. Herein, we present a patient with primary myelofibrosis who received ruxolitinib and developed disseminated cryptococcosis due to CN. We further discuss immune compromising factors indigenous to myeloproliferative neoplasms, ruxolitinib, and immunological pathways associated with janus kinase inhibition. We further review other cases of cryptococcal infections in patients receiving ruxolitinib reported in the literature. The report underscores the importance of suspecting infections with intracellular pathogens early in the course of illness in patients with higher rates of cumulative immunosuppression. A high clinical suspicion should be maintained when caring for such immunosuppressed patients receiving immunomodulatory agents as severe, disseminated infections can present atypically and lead to worse outcomes.
Occurrence of acute cholinergic syndrome in an infant due to donepezil ingestion
Authors: Sadeghi Mahdieh , Zakariaei Zakaria , Soleymani Mostafa , Malakian Abdollah 
Published: February 2022

AbstractDonepezil and acetyl cholinesterase inhibitors are the most commonly prescribed medications for the treatment of Alzheimer's disease. We describe a 2‐year‐old infant who was referred to the emergency department after developing cholinergic syndrome 6 hours after ingesting 10 mg of his grandmother's donepezil tablets and was finally discharged in stable condition.
Quantitative Operando Detection of Electro Synthesized Ammonia Using Mass Spectrometry
Authors: Krempl Kevin , Hochfilzer Degenhart , Cavalca Filippo , Saccoccio Mattia , Kibsgaard Jakob , Vesborg Peter C. K. , Chorkendorff Ib 
Published: February 2022

AbstractIn this work the successful operando detection of synthesized ammonia from nitrogen reduction in non‐aqueous electrolytes with an electron‐ionization mass spectrometer was reported. Using selective ionization at 22 eV together with a highly sensitive micro‐chip‐based electrochemistry mass spectrometry set‐up, quantitative detection of produced ammonia down to few pmol s−1 in non‐aqueous as well as aqueous electrolytes was demonstrated. The well‐defined electrochemical and mass transport environment of the thin‐layer electrochemical cell allowed for fundamental studies of the nitrogen reduction process, which was shown to produce ammonia at faradaic efficiencies of 49±3 % at ambient pressure. Moreover, through the operando capabilities of the developed method, it was shown that ammonia production proceeded even after lithium electroplating had been terminated This potentially explained why higher faradaic efficiencies of lithium mediated ammonia synthesis were observed under dynamic cycling conditions. Two possible mechanisms for this behavior were proposed and an outlook towards future research in operando studies of lithium mediated ammonia synthesis was given.
Rubbed PFN−Br as an Alignment Layer for Liquid Crystalline Conjugated Polymer Chain Orientation**
Authors: Wei Xuanxuan , Zhang Zhiyuan , Zhang Qian , Fu Shuai , Xia Ruidong 
Published: February 2022

AbstractWe report poly[(9,9‐bis(3’‐((N,N‐dimethyl)‐N‐ethylammonium)‐propyl)‐2,7‐fluorene)‐alt‐2,7‐(9,9‐dioctylfluorene)]dibromide (PFN−Br), a previously reported charge transfer interlayer for organic optoelectronic devices, as an orientation/alignment layer and rubbing as a means to achieve chain orientation for liquid crystalline conjugated polymers (LCCP). Polyfluorene and its derivatives, namely blue emission polymer poly(9,9‐dioctylfluorene) (PFO), green‐yellow emission polymer poly(9,9‐dioctylfluorene‐cobenzothiadiazole) (F8BT), and binary blend red emission polymer F8BT/Red F (Ref F=poly(triphenylamine‐co‐4,7‐di(thiophen‐2‐yl)benzo[c][1,2,5]thiadiazole‐co‐benzo[c]thiadiazole‐co‐9,9‐dioctyl‐9H‐fluorene)), demonstrated clear polarized absorption when PFN−Br was used as the orientation layer. We have studied the effects of rubbing times of a PFN−Br layer, and molecular weight, film thickness and annealing temperature of LCCP on the orientation degree. A high absorption anisotropy ratio of 12.22 was achieved for 106 nm thick F8BT film with a molecular weight of 55000 at the annealing temperature 265 °C, which is close to the theoretical limitation of the dichroic ratio of F8BT derived previously. Finally, we studied the amplified spontaneous emission (ASE) in chain oriented F8BT and PFO films. It showed that chain orientation made the ASE threshold anisotropic and lowers the threshold compared to the isotropic films if pumped under the favored configuration. This work provides a novel approach to reduce laser threshold and improve charge transfer simultaneously for electrically pumped devices when combined with the conductive property of PFN−Br.
Multiscale modeling of tumor adaption and invasion following anti‐angiogenic therapy
Authors: Cess Colin G. , Finley Stacey D. 
Published: February 2022

AbstractIn order to promote continued growth, a tumor must recruit new blood vessels, a process known as tumor angiogenesis. Many therapies have been tested that aim to inhibit tumor angiogenesis, with the goal of starving the tumor of nutrients and preventing tumor growth. However, many of these therapies have been unsuccessful and can paradoxically further tumor development by leading to increased local tumor invasion and metastasis. In this study, we use agent‐based modeling to examine how hypoxic and acidic conditions following anti‐angiogenic therapy can influence tumor development. Under these conditions, we find that cancer cells experience a phenotypic shift to a state of higher survival and invasive capability, spreading further away from the tumor into the surrounding tissue. Although anti‐angiogenic therapy alone promotes tumor cell adaptation and invasiveness, we find that augmenting chemotherapy with anti‐angiogenic therapy improves chemotherapeutic response and delays the time it takes for the tumor to regrow. Overall, we use computational modeling to explain the behavior of tumor cells in response to anti‐angiogenic treatment in the dynamic tumor microenvironment.
Copper Supported on Ceria Mesocellular Foam Silica as an Effective Catalyst for Reductive Condensation of Acetone to Methyl Isobutyl Ketone
Authors: Grzelak Kalina , Trejda Maciej , Riisager Anders 
Published: February 2022

AbstractCopper‐containing materials based on Ce‐ and Ca–Nb‐mesocellular foam (MCF) silica supports are prepared, characterized and applied as catalysts for gas‐phase reductive condensation of acetone to produce methyl isobutyl ketone (MIBK). The properties of the materials, the interaction of metal species, and their role in the catalytic process are examined by nitrogen physisorption, XRD, XPS, CO2‐TPD, H2‐TPR, and chemisorption of NO and pyridine combined with FTIR spectroscopy. A synergistic interaction of Cu2+, Cu0, and CeO2 species incorporated in the MCF support enable the Cu/Ce‐MCF catalyst to yield 34 % of acetone conversion with over 90 % MIBK selectivity at 250 °C. Moreover, this high catalyst selectivity is maintained during operation for 24 h despite a decline in catalyst activity. The catalytic performance is superior to that of hydroxyapatite‐supported Cu and similar previously reported Pd‐containing catalysts.
A tri‐band triangular lamp post shaped quad port MIMO antenna
Authors: Naidu Praveen Vummadisetty , Mahesh Babu Dhanekula , Akkapanthula Sai Haranadh , Kumar Arvind , Vummadisetty Neelima , Kumar Sanjeev 
Published: February 2022

SummaryIn this article, a novel and compact multiband quad port asymmetric coplanar strip‐fed triangular lamp post shaped printed multiple‐input and multiple‐output (MIMO) antenna has been presented. The proposed MIMO antenna is designed and developed on a FR‐4 (εr = 4.4) dielectric (40 × 40 × 1.6) mm3. The proposed MIMO antenna utilizes the pattern diversity technique to obtain the desired isolation among closely spaced radiators. Simulated and measured results indicate that |S11| of proposed lamp post shaped MIMO antenna array < −10 dB, that assures the operation in three frequency bands, namely, (2.27–2.42), (3.38–3.62), and (4.5–6.23) GHz, which are the operating bands of WiBro, Bluetooth, WiMAX, and WLAN/WiMAX, respectively. A peak radiation gain of 5.1 dBi is observed alongside with a maximal radiation efficiency close to 92% in operating band for the proposed MIMO antenna. A minimal isolation of −18 dB between the antenna elements has been observed in all three operating bands, which is necessary for improved performance to achieve high data transfer rates. Performance critical diversity characteristics such as diversity gain (DG), envelope correlation coefficient (ECC), total active reflection coefficient (TARC), mean effective gain (MEG), and channel capacity (CC) of the compact proposed lamp post shaped MIMO antenna have been calculated and found that the correlation coefficient was <0.001 with higher diversity gain > 9.95 dB and achieved better CCL < 0.4 bits/s/Hz in the respective operating bands. With these results, the proposed triangular lamp post shaped MIMO array is suitable for high data rate applications, which is one of the main concerns of 5G and beyond networks.
A scoping review of the treatment of hypertrophic scars and keloids
Authors: Davies Benjamin M. , Carter Alison R. , Brindley David A. 
Published: February 2022

AbstractHypertrophic scars and keloids are both conditions that represent an abnormal healing response by skin in response to some form of trauma. They are a cause of significant morbidity and psychological distress to patients. The costs associated with treating these conditions are considerable and had already reached 12 billion US dollars per annum by 2009. The lack of a well‐established treatment to reliably and effectively treat these conditions is demonstrated by the multitude of different treatments available. This scoping review aimed to review the treatment landscape available for hypertrophic scars and keloids to provide an overview of this varied field. A total of 113 studies representing 5211 participants were located. We identified over 40 different treatments utilised in the treatment of established scars and prophylactically to prevent scarring of wounds and burns. The heterogeneity of study design in terms of control arms, comparators, and outcome measures means that direct comparison of studies across the identified studies is difficult. While a large number of interventions were shown to have a beneficial effect compared with no treatment, there was no clear treatment that was both sufficiently effective and useable to have achieved widespread usage. This study highlights the wide variety of treatments available for hypertrophic scars and keloids. Future work will concentrate on more specific questions which will lend themselves to more detailed investigation using techniques such as network meta‐analysis. Future studies of treatments for hypertrophic scars and keloids should also ensure they are designed to allow for easy comparison with other studies.
Seismic pounding damage to adjacent reinforced concrete frame–shear wall buildings and freestanding contents
Authors: Lu Yang , Xiong Feng , Ran Ming‐Ming , Ge Qi , Wang Jianze 
Published: February 2022

AbstractClosely spaced tall buildings are common in modern urban areas due to limited supply of land to accommodate rapid growth of population. These buildings are vulnerable in seismically active regions particularly when earthquake‐induced pounding occurs between adjacent buildings, resulting in high floor acceleration spikes that may lead to excessive demands on building contents (BCs). This paper examines the effect of seismic pounding on damage to adjacent reinforced concrete (RC) frame–shear wall buildings and their freestanding contents using nonlinear response‐history analyses (RHA). A cascading analysis approach is adopted to obtain absolute floor accelerations of pounding tall buildings, which are then applied to determine response of freestanding contents dominated by either sliding or rocking motions. In order to provide a useful tool for practicing engineers and facilitate analyses, the buildings are simplified as story‐based multiple‐degree‐of‐freedom (MDOF) flexural‐shear models, and unanchored contents are idealized as single‐degree‐of‐freedom (SDOF) oscillators, all of which are approximated using OpenSees and verified against solutions to their exact equations of motion solved using Matlab. It is shown that seismic pounding leads to higher building damage and alters story damage distribution patterns. It is concluded that pounding significantly increases sliding potential and maximum sliding displacement demands on stocky contents, especially those on higher floors. The transitions of stick–slip response of the contents coincide well with pounding occurrences. It is also concluded that pounding has detrimental effects on slender rocking contents, but rocking rotation histories of these contents do not show abrupt changes upon impact.