bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nutrient driven transcriptional changes during phage infection in an aquatic Gammaproteobacterium
Authors: Nilsson Emelie , Li Ke , Hoetzinger Matthias , Holmfeldt Karin 
Published: January 2022

SummaryPhages modulate bacterial metabolism during infection by regulating gene expression, which influences aquatic nutrient cycling. However, the effects of shifting nutrient regimes are less understood. Here, we analyzed transcriptomes of an ecologically relevant Gammaproteobacterium and its lytic phage in high (HNM) and low (LNM) nutrient medium. Despite different infection characteristics, including reduced burst size and longer latent period in LNM, the phage had a fixed expression profile. Bacterial transcription was instead different depending on nutrient regime, with HNM bacteria focusing on growth while LNM bacteria focused on motility and membrane transport. Additionally, phage infection had a larger effect on bacterial gene expression in LNM compared to HNM, e.g. suppressing increased iron uptake and altering expression of phosphorus uptake genes. Overall, phage infection influenced host metabolism more in LNM, which was more similar to natural conditions, emphasizing the importance of considering natural conditions to understand phage and host ecology.
Predation of the Balkan frog Pelophylax kurtmuelleri (Gayda, 1940) (Anura: Ranidae) by the giant water bug Lethocerus patruelis (Stål, 1854) (Hemiptera: Heteroptera: Belostomatidae)
Authors: Christopoulos Apostolos , Daskalaki Hera , Vlachopoulos Konstantinos , Pafilis Panayiotis 
Published: January 2022

AbstractLethocerinae water bugs are vertebrate predators that include in their diet large prey such as fish, turtles, snakes and frogs. Although frog consumption is common among the members of the genus Lethocerus, similar reports are unknown from Europe. Here we report a predation event by an adult Lethocerus patruelis (Stål, 1854) on a subadult Balkan water frog Pelophylax kurtmuelleri (Gayda, 1940), the first description of its kind in Europe.
Thiamine‐dependent regulation of mammalian brain pyridoxal kinase in vitro and in vivo
Authors: Bunik Victoria , Aleshin Vasily , Nogues Isabel , Kähne Thilo , Parroni Alessia , Contestabile Roberto , Di Salvo Martino Luigi , Graf Anastasia , Tramonti Angela 
Published: January 2022

AbstractVitamins B1 (thiamine) and B6 (pyridox (al/ine/amine)) are crucial for central nervous system (CNS) function and neurogenesis due to the coenzyme action of their phosphorylated derivatives in the brain metabolism of glucose and neurotransmitters. Here, the non‐coenzyme action of thiamine on the major mammalian producers of pyridoxal‐5′‐phosphate (PLP), such as pyridoxal kinase (PdxK) and pyridoxine 5′‐phosphate oxidase (PNPO), is characterized. Among the natural thiamine compounds, thiamine triphosphate (ThTP) is the best effector of recombinant human PdxK (hPdxK) in vitro, inhibiting hPdxK in the presence of Mg2+ but activating the Zn2+‐dependent reaction. Inhibition of hPdxK by thiamine antagonists decreases from amprolium to pyrithiamine to oxythiamine, highlighting possible dysregulation of both the B1‐ and B6‐dependent metabolism in the chemical models of thiamine deficiency. Compared with the canonical hPdxK, the D87H and V128I variants show a twofold increase in Kapp of thiamine inhibition, and the V128I and H246Q variants show a fourfold and a twofold decreased Kapp of thiamine diphosphate (ThDP), respectively. Thiamine administration changes diurnal regulation of PdxK activity and phosphorylation at Ser213 and Ser285, expression of the PdxK‐related circadian kinases/phosphatases in the rat brain, and electrocardiography (ECG). In contrast to PdxK, PNPO is not affected by thiamine or its derivatives, either in vitro or in vivo. Dephosphorylation of the PdxK Ser285, potentially affecting mobility of the ATP‐binding loop, inversely correlates with the enzyme activity. Dephosphorylation of the PdxK Ser213, which is far away from the active site, does not correlate with the activity. The correlations analysis suggests the PdxK Ser213 to be a target of kinase MAP2K1 and phosphatase Ppp1ca. Diurnal effects of thiamine administration on the metabolically linked ThDP‐ and PLP‐dependent enzymes may support the brain homeostatic mechanisms and physiological fitness.
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Association between coffee drinking and endometrial cancer risk: A meta‐analysis
Authors: Gao Yu , Zhai Peiling , Jiang Fang , Zhou Fushun , Wang Xinbo 
Published: January 2022

AbstractAimCoffee drinking is considered as a risk factor of endometrial cancer (EC). Here, we conducted a meta‐analysis of observational study to evaluate the relationship between coffee drinking and the risk of EC.MethodsThe MEDLINE and EMBASE databases were searched until July 2018. Pooled relative risks (RRs) with 95% confidence intervals (CIs) were calculated using a random‐effects model.ResultsA total of 24 studies (12 case–control and 12 cohort studies) on coffee intake with 9833 incident cases of EC and 699 234 subjects were included in the meta‐analysis. The pooled RR of endometrial cancer for the highest versus the lowest categories of coffee intake was 0.71 (95% CI: 0.65–0.77; I2 = 14%, p for heterogeneity = 0.26). By study design, the pooled RRs were 0.68 (95% CI: 0.56–0.83) for case–control studies and 0.70 (95% CI: 0.63–0.77) for cohort studies. For different regions, the pooled RRs were 0.74 (95% CI: 0.62–0.88) in Europe, 0.71 (95% CI: 0.64–0.79) in United States/Canada, and 0.40 (95% CI: 0.28–0.57) in Japan. By additional subgroup analysis, a stronger inverse association was shown in caffeinated coffee drinkers (RR 0.66, 95% CI: 0.52–0.83), individuals with the higher body mass index (BMI) (RR 0.65, 95% CI: 0.54–0.79), never smokers (RR 0.68, 95% CI: 0.56–0.84), ever smokers (RR 0.56, 95% CI: 0.45–0.70), and those who never used hormone replacement therapy (HRT) (RR 0.88, 95% CI: 0.79–0.98). The consumption of filtered or boiled coffee showed no significant association.ConclusionsIncreased coffee intake is associated with a reduced risk of EC.
The effect of pneumoperitoneum and Trendelenburg position on optic nerve sheath diameter in patients undergoing laparoscopic hysterectomy
Authors: Bayramov Tural , Kilicaslan Banu , Akinci Seda B. , Boyraz Gokhan 
Published: January 2022

AbstractBackground and AimLaparoscopic hysterectomy (LH) have been frequently used because of low complication rates and short duration of hospital stay. Elevated intracranial pressure (ICP), a disadvantage of laparoscopic surgery, is caused by the Trendelenburg position (TP) and CO2 pneumoperitoneum (PP). This study aimed to evaluate TP and PP associated changes in ICP by ONSD measurements during LH. The intra‐and inter‐observer consistency and reliability of ONSD measurements were also investigated.MethodsSixty patients with were enrolled into this prospective study. ONSD for each patient was measured by three anesthesiologists at T0, T1, T2, and T3 time points. ONSD, mean arterial pressure (MAP), end tidal CO2 (EtCO2), and arterial blood CO2 partial pressure values (PaCO2) were measured at T0: baseline, T1: 10 min after introducing 20 mmHg PP, T2: 10 min after placing the patient in TP and 15 mmHg PP and, T3: 10 min after PP deflation.ResultsThe ONSD measured at T1 (5.97 ± 0.49 cm) and T2 (5.95 ± 0.57 cm) were higher than T0 (5.63 ± 0.53 cm) and T3 (5.72 ± 0.47 cm) (p < 0.05). There were no correlations between MAP and ONSD, and also between PaCO2, EtCO2, and ONSD measurements at any time points. Inter‐observer intraclass correlation coefficient (ICC) values of ONSD measurements by all examiners had moderate (at T1) to good (at T0, T2, T3) reliability. Intra‐observer agreements were reasonable for each observer.ConclusionONSD measurements increase with CO2PP and TP in patients undergoing LH. Transorbital sonography is a reliable method to monitor intraoperative changes in ONSDs. This study underlines the need for careful training and the importance of standardization in order to obtain reliable results in the examination technique of ONSD measurements.
Dark respiration rates are not determined by differences in mitochondrial capacity, abundance and ultrastructure in C4 leaves
Authors: Fan Yuzhen , Scafaro Andrew P. , Asao Shinichi , Furbank Robert T. , Agostino Antony , Day David A. , von Caemmerer Susanne , Danila Florence R. , Rug Melanie , Webb Daryl , Lee Jiwon , Atkin Owen K. 
Published: January 2022

AbstractOur understanding of the regulation of respiration in C4 plants, where mitochondria play different roles in the different types of C4 photosynthetic pathway, remains limited. We examined how leaf dark respiration rates (Rdark), in the presence and absence of added malate, vary in monocots representing the three classical biochemical types of C4 photosynthesis (NADP‐ME, NAD‐ME and PCK) using intact leaves and extracted bundle sheath strands. In particular, we explored to what extent rates of Rdark are associated with mitochondrial number, volume and ultrastructure. Based on examination of a single species per C4 type, we found that the respiratory response of NAD‐ME and PCK type bundle sheath strands to added malate was associated with differences in mitochondrial number, volume, and/or ultrastructure, while NADP‐ME type bundle sheath strands did not respond to malate addition. In general, mitochondrial traits reflected the contributions mitochondria make to photosynthesis in the three C4 types. However, despite the obvious differences in mitochondrial traits, no clear correlation was observed between these traits and Rdark. We suggest that Rdark is primarily driven by cellular maintenance demands and not mitochondrial composition per se, in a manner that is somewhat independent of mitochondrial organic acid cycling in the light.
Molecular cues of sugar signaling in plants
Authors: Choudhary Anuj , Kumar Antul , Kaur Nirmaljit , Kaur Harmanjot 
Published: January 2022

AbstractSugars, the chemically bound form of energy, are formed by the absorption of photosynthetically active radiation and fixation in plants. During evolution, plants availed the sugar molecules as a resource, balancing molecule, and signaling molecule. The multifaceted role of sugar molecules in response to environmental stimuli makes it the central coordinator required for growth, survival, and continuity. During the course of evolution, the molecular networks have become complex to adapt or acclimate to the changing environment. Sugar molecules are sensed both intra and extracellularly by their specific sensors. The signal is transmitted by a signaling loop that involves various downstream signaling molecules, transcriptional factors and, most pertinent, the sensors TOR and SnRK1. In this review, the focus has been retained on the significance of the sugar sensors during signaling and induced modules to regulate plant growth, development, biotic and abiotic stress. It is interesting to visualize the sugar molecule as a signaling unit and not only a nutrient. Complete information on the downstream components of sugar signaling will open the gates for improving the qualitative and quantitative elements of crop plants.
Evaluation of TNF‐α and IL‐6 in saliva among diabetic retinopathy patients in East Coast Malaysia
Authors: Khairul‐Anwar Ibrahim , Wan‐Nazatul‐Shima Shahidan , Siti‐Lailatul‐Akmar Zainuddin , Siti‐Azrin Ab Hamid , Zunaina Embong 
Published: January 2022

AbstractObjectiveTo compare tumour necrosis factor‐alpha (TNF) and interleukin (IL)‐6 levels in saliva between different stages of diabetic retinopathy (DR).MethodsThis comparative cross‐sectional study was conducted between January 2018 and November 2020. This study included diabetes mellitus (DM) patients with no DR, non‐proliferative DR (NPDR), and proliferative DR (PDR). None of the patients with DM were included in the control group. Unstimulated saliva samples were then collected. TNF‐α and IL‐6 levels were measured.ResultsAltogether, 120 patients were included in the study (DM without DR, 33 patients; DM with NPDR, 30 patients; DM with PDR, 32 patients; non‐DM, 25 patients). The mean IL‐6 level in saliva was significantly higher in the DM group (0.033 ± 0.005 pg/ml) than in the non‐DM group (0.027 ± 0.001 pg/ml) (p < 0.001 after adjusting for covariates). There was no significant difference in the mean salivary TNF‐α between patients with DM and those without DM after adjusting for covariates. The mean IL‐6 in saliva was significantly higher in the NPDR (0.036 ± 0.003 pg/ml) and PDR (0.093 ± 0.023 pg/ml) groups than in the no DR group (0.027 ± 0.001 pg/ml) (p < 0.001 and p < 0.001, respectively). Mean TNF‐α in saliva was significantly higher in the NPDR (0.086 ± 0.022 pg/ml) and PDR (0.093 ± 0.023 pg/ml) groups than in the no DR group (0.049 ± 0.011 pg/ml) (p = 0.015 and p = 0.003, respectively).ConclusionThere is an association between inflammatory biomarkers in saliva (IL‐6 and TNF‐α) and severity of DR among patients with DM, suggesting that these salivary biomarkers are potential biomarkers for screening, monitoring, and predicting the progression of DR.
A standardized multi‐method survey to enhance characterization of riparian invertebrate communities
Authors: Webb Jonathan R. , Gething Kieran J. , Kratz Christoph C. , England Judy , Drewitt Allan L. , Stubbington Rachel 
Published: January 2022

AbstractRiparian invertebrate communities are species rich, but variability in sampling methods hampers assessment of their distributions and inference of the quality of their habitats. To reduce this variability, a standardized, multi‐method survey protocol was recently developed. Here, riparian beetle community surveys conducted before and after the protocol's introduction were used to evaluate its effectiveness in characterizing communities and in limiting variability among surveys. Use of the standardized protocol reduced variability in sampling effort, and this had a limited effect on estimates of taxonomic richness. Surveys using the protocol captured significantly more species than surveys done before its introduction, evidencing the benefits of standardized multi‐method survey protocols. Our study highlights that standardized multi‐method survey protocols may enable identification and prioritization of sites requiring management to improve habitat quality. As such, we recommend the integration of such protocols into monitoring programmes, to enhance protection of biodiverse invertebrate communities in vulnerable riparian habitats.
A retrospective cohort study of three wound catheter infusion analgesia regimes after midline laparotomy
Authors: Perry Jonathan , Byth Karen , Bullingham Alan 
Published: January 2022

BackgroundLocal anesthetic wound infusion catheters are increasingly used postmidline laparotomy to reduce pain and opioid use, however there is little evidence to support any particular infusion regime.MethodsA retrospective cohort study was undertaken of patients after midline laparotomy who had bilateral local anesthetic wound infusion catheters surgically placed. Patients were recruited into 3 cohorts: ropivacaine 0.2% 5–8 mL/hour continuous wound infusion, 10 mL programmed intermittent bolus 2 hourly, 20 mL programmed intermittent bolus 4 hourly. The primary outcome was the maximum daily Numerical Rating Pain Score with movement (dynamic pain score) recorded during first 96 hours postprocedure. Secondary outcomes included the maximum daily resting pain score and opioid utilization.ResultsIn three cohorts of 70 patients (n=210), the maximum daily dynamic pain score in the intermittent bolus 2-hourly and 4-hourly cohorts was lower when compared with the continuous infusion group over the first 4 postoperative days. The mean difference in maximum daily pain score with respect to the continuous infusion regime was 0.8 (95% CI 0.2 to 1.4) for the intermittent bolus 2-hourly group and 0.6 (95% CI 0.0 to 1.2) for the 4-hourly group. Generalized estimating equation modeling indicates the reduction in dynamic pain score is greatest with the intermittent bolus 2-hourly regime over the first 72 hours postprocedure. The 2-hourly intermittent bolus regime was also associated with lower opioid utilization and local anesthetic exposure.ConclusionsIntermittent wound infusion catheter infusion regimes were associated with lower maximum daily dynamic pain scores, although the magnitude of this change may be of limited clinical significance.
One Strike, You’re Out: Lightning during Professional Baseball Games
Authors: Vagasky Chris 
Published: January 2022

Abstract
Millions of people attend Major League Baseball games every year, during a season that is played primarily outdoors at the peak of the U.S. lightning season. In recent years, social media photographs and baseball game television broadcasts have revealed lightning within proximity of several baseball games without the game being delayed. Lightning data from the U.S. National Lightning Detection Network within 12.8 km of 9717 Major League Baseball games between 2016 and 2019 were examined to find the extent to which lightning is a threat to games, players, staff, and fans: 717 games were found to have lightning within 12.8 km, with more than 175 000 in-cloud and cloud-to-ground lightning discharges detected during those games. The distribution of games with lightning was not uniform and is related to the annual average lightning density of each ballpark. Despite the significant risk of a lightning-related incident at Major League Baseball games, existing work from other organizations like the National Collegiate Athletics Association and the National Athletics Trainers Association can be leveraged to improve lightning safety at professional baseball games.

Significance Statement
Nearly one of every 14 Major League Baseball games has lightning within what lightning safety experts would consider an unsafe distance. The potential for a lightning casualty incident is high because most games are played outdoors and millions of people are at baseball games every year. Although frameworks that can improve lightning safety at the thousands of professional baseball games that are played every year exist, it is unclear how frequently they are implemented.
Detect the early-warning signals of diseases based on signaling pathway perturbations on a single sample
Authors: Huo Yanhao , Zhao Geng , Ruan Luoshan , Xu Peng , Fang Gang , Zhang Fengyue , Bao Zhenshen , Li Xin 
Published: January 2022

Abstract
Background
During the pathogenesisof complex diseases, a sudden health deterioration will occur as results of the cumulative effect of various internal or external factors. The prediction of an early warning signal (pre-disease state) before such deterioration is very important in clinical practice, especially for a single sample. The single-sample landscape entropy (SLE) was proposed to tackle this issue. However, the PPI used in SLE was lack of definite biological meanings. Besides, the calculation of multiple correlations based on limited reference samples in SLE is time-consuming and suspect.

Results
Abnormal signals generally exert their effect through the static definite biological functions in signaling pathways across the development of diseases. Thus, it is a natural way to study the propagation of the early-warning signals based on the signaling pathways in the KEGG database. In this paper, we propose a signaling perturbation method named SSP, to study the early-warning signal in signaling pathways for single dynamic time-series data. Results in three real datasets including the influenza virus infection, lung adenocarcinoma, and acute lung injury show that the proposed SSP outperformed the SLE. Moreover, the early-warning signal can be detected by one important signaling pathway PI3K-Akt.

Conclusions
These results all indicate that the static model in pathways could simplify the detection of the early-warning signals.
Machine Learning Analysis of Literature Data on the Water Gas Shift Reaction toward Extrapolative Prediction of Novel Catalysts
Authors: Mine Shinya , Jing Yuan , Mukaiyama Takumi , Takao Motoshi , Maeno Zen , Shimizu Ken-ichi , Takigawa Ichigaku , Toyao Takashi 
Published: January 2022

Abstract
Literature data based on the water gas shift (WGS) reaction have been analyzed using statistical methods based on machine learning (ML). Our ML approach, which considers elemental features as input representations rather than the catalyst compositions, was successfully applied, and new promising catalyst candidates for future research were proposed.
Polyfactorial approach in rehabilitation of football players' muscles after sports injuries
Authors: Vladymyrov Oleksandr , Chukhraiev Nikolay , Shmorgun Аndriy , Zukow Walery , Мaliuta Vladimir , Chukhraieva Olena 
Published: January 2022

The article discusses a multifactorial approach to the restoration of muscle volume and rehabilitation of football players after sports injuries, which includes the implementation of electric myostimulation in a magnetic field in combination with the effect of electromagnetic radiation of the visible or infrared spectrum on muscles and the area of injury. Comparative studies were carried out in two groups of footballers in rehabilitation after ankle injury rehabilitation. The medical rehabilitation of the players of the 1st group was carried out according to the protocol approved in the center: the MIT-FM apparatus, the protocol for the implementation of rehabilitation provides for the implementation of drug treatment and physiotherapeutic procedures based on the combined use of therapy with electromagnetic waves of the red, blue and infrared spectral range with a power of 50 mW in a pulsed magnetic field of intensity 30 mT and a pulse repetition rate of 9.4 Hz.
In group 2, the rehabilitation of footballers after injuries of the lower extremities was carried out according to the protocol developed by the authors: sequential connection of the rehabilitation protocol of the first group and combined magneto-photo electric myostimulation of muscles with myotonic impulses, with a pulse repetition rate of 9.4 Hz. The repetition period of the packs of impulses is 8 seconds with the duty cycle of repetition of the packs of impulses 2. The current strength was set individually for each patient - until the appearance of visible muscle contractions.
Muscle photostimulation was performed with electromagnetic radiation of red (wavelength 0.63 μm, power 30 mW), blue (wavelength 0.45 μm, power 30 mW) and infrared (wavelength 0.75 μm, power 50 mW) spectral ranges in constant magnetic field (intensity 30 mT).
The first electrode is placed on the muscle's innervation zone. The second electrode provides a combined effect of electrical impulses and optical flux in a constant magnetic field on the abdomen of the muscle according to the labile technique.
The time of one procedure was 40 minutes. The number of procedures for a course of treatment is 15.
As a result, the analysis of the data obtained (assessment of the quality of life according to the extended table SF-36) revealed a positive dynamics of clinical manifestations in both groups. The main result of the research is the development of a protocol for the rehabilitation of football players after injuries of the lower extremities based on the use of drug treatment, physiotherapy procedures based on the combined use of therapy with electromagnetic waves of the blue and infrared spectrum in a constant magnetic field in combination with muscle electrostimulation, which has a higher level rehabilitation potential versus applicable footballerІ rehabilitation protocols.
Shoe Reels: The History and Philosophy of Footwear in Film, Elizabeth Ezra and Catherine Wheatley (Eds) (2020)
Authors: Roberts Cheryl 
Published: January 2022

Review of: Shoe Reels: The History and Philosophy of Footwear in Film, Elizabeth Ezra and Catherine Wheatley (Eds) (2020)
Edinburgh: Edinburgh University Press, 320 pp.,
ISBN 978-1-47445-140-6, h/bk, £85