bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictors of intra-hospital mortality in patients with acute heart failure and type 2 diabetes seen at the Soavinandriana Hospital Center: a retrospective study
Authors: Raharinavalona Sitraka Angelo , Raherison Rija Eric , Razanamparany Thierry , Ralamboson Solofo Andrianarivelo , Andrianasolo Radonirina Lazasoa , Rakotomalala Andrianirina Dave Patrick 
Published: August 2022

Abstract
Background
The concomitant presence of acute heart failure (AHF) and diabetes is a major cause of morbidity and mortality. Our study aims to determine the characteristics of AHF in patients with type 2 diabetes and the predictors of intra-hospital mortality.
Methods
This was a descriptive and analytical retrospective study, carried out in the Internal Medicine and Cardiovascular Diseases departments of the Soavinandrina Hospital Center over a period of 2 years. The diagnosis of AHF and diabetes was made according to the criteria of the European Society of Cardiology and the American Diabetes Association, respectively.
Results
The prevalence of AHF in type 2 diabetes was 16.9%. With a male predominance, the mean age of the patients was 61.6 ± 10.2 years. The average of diabetes duration was 5.5 ± 4.5 years, and which was unbalanced in 82.5%. AHF was mainly manifested by breathlessness (88.9%), bilateral pulmonary crepitations (79.4%) and ankle swelling (71.4%). Left ventricular ejection fraction (LVEF) was reduced in 69.8% of cases. AHF was mostly decompensated by infections (49.2%). With a rate of 6.3%, intra-hospital mortality was significantly correlated with the average of diabetes duration (p = 0.0002), history of heart disease (p = 0.0032), absence previous basic treatment for heart failure (Angiotensin-converting enzyme inhibitors, beta-blockers, diuretics, p < 0.05), arterial oxygen desaturation in ambient air ≤ 90% (p = 0.0106), severity breathlessness (p = 0.0398) and decrease in LVEF (p = 0.0140).
Conclusion
AHF in patients with type 2 diabetes is frequent and serious, requiring early, adequate, and multidisciplinary management. Therapeutic education and infection prevention are essential.
Household level of Air Pollution and its impact on the occurrence of Acute Respiratory Illness among children under five: Secondary analyses of Demographic and Health Survey in West Africa
Authors: DAFFE Mouhamadou Lamine , THIAM Salimata , BAH Fatoumata , NDONG Awa , Cabral Mathilde , DIOP Cheikh , TOURE Aminata , LAM Absa , FALL Mamadou 
Published: August 2022

Abstract
Background
One in ten deaths of children under five are attributable to indoor air pollution, and Acute Respiratory Illness (ARI) are a direct cause.
Objective
This study made it possible to characterize indoor air pollution in West African Economic and Monetary Union (WAEMU) area and to estimate its impact on occurrence of ARI in children under five.
Methods
This is a secondary analysis on dataset from WAEMU member states’ Demographic and Health Survey (DHS). Pollution is characterized by using a composite variable called “Household level of air pollution”, created from questions related to degradation factors of indoor air quality (domestic combustion processes) and impact measurement was carried out by logistic regression.
Results
Burkina Faso stands out with a greater number of households with a high level of pollution (63.7%) followed by Benin (43.7%) then Togo (43.0%). The main exposure factor "Household level of air pollution" was only associated with ARI in Togo (prevalence: 51.3%; chi-2: p-value < 0.001). Exposure to high level of pollution constitutes an excess risk, although it is not significant (Adjusted Odds Ratio (AOR): 1.13, 95% [0.66–1.92]) and this could be explained by the highly infectious etiology of the ARI.
Rapid method for simultaneous determination of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid isomers in milk by GC-FID
Authors: Porto Brenda Lee Simas , Faria Isaura Daniele Leite , Souza Jéssica Cordeiro Queiroz , Moreira Olívia Brito de Oliveira , Gama Marco Antônio Sundfeld , Oliveira Marcone Augusto Leal 
Published: August 2022

Abstract
Ruminant milk is the main source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among CLA isomers in milk fat. Small amounts of trans-10, cis-12 CLA is also found in synthetic supplements, predominantly. As both isomers being biologically active with potential health benefits, there is great interest in quantifying them in both natural and synthetic sources. An alternative method for the analysis of cis-9, trans-11 and trans-10, cis-12 CLA in milk by fast gas chromatography (GC) has been proposed in the present study. Milk fat was extracted and the fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a gas chromatograph equipped with a flame ionization detector (GC-FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m x 0.10 mm x 0.08 µm). Separation of cis-9, trans-11 and trans-10, cis-12 CLA isomers was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by standard addition and the results were compared with those obtained using the traditional 100-m long CP-SIL88 capillary column, with no significant differences being detected within 95% confidence interval. The proposed method allowed for the rapid quantification of cis-9, trans-11 and trans-10, cis-12 CLA isomers by GC-FID, which could be particularly useful in focused regulatory routines of a large number of samples with greater analytical frequency.
Indocyanine green fluorescence imaging during laparoscopic rectal cancer surgery could reduce the incidence of anastomotic leakage: A single institutional retrospective cohort study
Authors: Kondo Akihiro , Kumamoto Kensuke , Asano Eisuke , Feng Dongping , Kobara Hideki , Okano Keiichi 
Published: August 2022

Abstract
Background: There is insufficient evidence on whether indocyanine green (ICG) fluorescence angiography can reduce the incidence of anastomotic leakage (AL). This retrospective cohort study aimed to evaluate the effect of ICG fluorescence angiography on AL rates in laparoscopic rectal cancer surgery at a single institution.Methods: Patients who underwent laparoscopic low anterior resection or intersphincteric resection with ICG fluorescence angiography (ICG group; n=73) and patients who underwent a similar surgical procedure for rectal cancer without ICG fluorescence (non-ICG group; n=114) were enrolled consecutively in this study. ICG fluorescence angiography was performed prior to transection of the proximal colon, and anastomosis was performed with sufficient perfusion using ICG fluorescence imaging. AL incidence was compared between both groups, and the risk factors for AL were analyzed. Results: AL occurred in 3 (4.1%) and 14 (12.3%) patients in the ICG and non-ICG groups, respectively. In the ICG group, the median perfusion time from ICG injection was 34 s, and 5 patients (6.8%) required revision of the proximal transection line. None of the patients requiring revision of the proximal transection line developed AL. In the univariate analysis, longer operating time (odds ratio: 2.758; 95% confidence interval: 1.023–7.624) and no implementation of ICG fluorescence angiography (odds ratio: 3.266; 95% confidence interval: 1.038–11.793) were significant factors associated with AL incidence, although the creation of a diverting stoma or insertion of a transanal tube was insignificant.Conclusion: ICG fluorescence angiography was associated with a significant reduction in AL during laparoscopic rectal cancer surgery. Changes in the surgical plan due to ICG fluorescence visibility may help improve the short-term outcomes of patients with rectal cancer.
The survey of Capparis extracts effects on expressional profile of essential self-renewal genes in MCF7 cell line
Authors: Marji Faezeh Askarian , Javid Ameneh , Noroozi Mojgan , Mirzaei Mohammad Reza 
Published: August 2022

Abstract
Several drugs have been proposed for the treatment of breast cancer, but none has fully treated the disease, so far. this study was to investigate the effect of aqueous-alcoholic extract of unripe Capparis fruit as an anticancer agent on expressional pattern of OCT4, NANOG and SOX2 as essential self-renewal pathway genes in MCF7 cell line (human breast carcinoma). MCF7 cells were cultured in RPMI-1640 medium, consisting of different concentrations of aqueous-alcoholic extract of unripe Capparis fruit (125, 250, 500, 1000, 1500, 2000, 2500 and 5000 μg/ml) for 48 and 72 hours. MTT assay was used to determine the cell proliferation inhibition (IC50). RT-PCR method was carried out to assess the fold changes of OCT4, NANOG and SOX2 genes. One-way ANOVA was used for the statistical analysis of obtained data. Hydroalcoholic extract of the unripe Capparis fruit caused time- and concentration-dependent cell death in MCF7 cells. IC50 was observed at 48h culture period with 4817.51 μg/mL , and 72h with 2724.29 μg/ml fruit extract, respectively. Our results have shown that cell death was induced by increasing Capparis extract concentration. According to RT-PCR findings on capparis extract-treated cells, the mean expression of OCT4, NANOG and SOX2 genes decreased after 48 and 72h of incubation with IC50 concentration compared to controls.Capparis plant species is able to decrease the expression of self-renewal genes in MCF7 cell line. Therefore, the Capparis extract can be considered as a promising candidate for the management of human breast cancer after clinical trials.
The dysregulation of NME1, LINC00511, and LINC01028 could disturb the regular apoptosis process and promote breast cancer in the Isfahan population: integrated bioinformatics and experimental analyses
Authors: Bakhshayesh Mahdieh , Chamgordani Parisa Rabiei , Sanaei Shima , Farhadi Haniyeh , Omidghaemi Shadi , Azadeh Mansoureh 
Published: August 2022

Abstract
Background: NME1 is a crucial gene in the proliferation, cancer survival, and differentiation processes. The abnormal changes in the expression of NME1 could cause disturbance in the mentioned processes and lead the normal cells to different abnormalities, including cancer. Breast cancer is the most frequently diagnosed cancer in women and ranks second among causes for cancer related death in women. In this study, the expression pattern of NME1 and the two lncRNAs that have possible interaction with NME1 (LINC01028 and LINC00528) had been evaluated In the Isfahan breast cancer (BC) patients.Methods and results: Microarray, pathway enrichment, Gene Ontology, RNA and Protein interaction, and co-expression analyses were performed to find novel integrated genes, proteins, and RNAs (coding and non-coding) in the BC patients, based on the bioinformatics tools (R programming language and online databases). After finding novel genes and RNAs, a real-time PCR experiment was performed to evaluate the accurate relative expression of found RNAs in the Isfahan human breast cancer samples. Bioinformatics analyses revealed that NME1 protein could regulate apoptotic-related proteins. Also, based on ENCORI and microarray data analysis, the expression of NME1 and LINC00511 (FC: 6.49, FDR < 0.0001) have a significant up-regulation in the BC samples. Also, LINC01028 had a significantly low expression in the mentioned datasets (FC: 0.08, FDR < 0.0001). A real-time PCR experiment validated the bioinformatics results. Based on the experiment, NME1 (logFC: 2.664, P. Value < 0.0001) and LINC00511 (logFC: 2.058, P. Value: 0.0180). Also, LINC01028 have significant low-expression in the BC samples (logFC: -2.219, P. Value: 0.0006).Conclusion: Based on the bioinformatics and experimental analyses, NME1, LINC00511, and LINC01028 could be three prognostic biomarkers in the Isfahan BC population. The disturbance of the expression level of mentioned RNAs can change the regular apoptosis process and promote BC development in the patients.
Self-care behaviours and associated factors among type 2 diabetes patients in Ghana: A systematic review
Authors: Opoku Richmond , Ackon Solomon Kwesi , Kumah Emmanuel , Botchwey Charles Owusu-Aduomi , Appiah Nana Esi , Korsah Shadrach , Peprah Michael 
Published: August 2022

Abstract
Introduction: Diabetes remains an emerging public health challenge in Ghana. The control of cardio‑metabolic risk factors among type 2 diabetes patients in the country is suboptimal, leading to massive healthcare costs. This systematic review examined the extent of adherence to self-care behaviours and associated factors among type 2 diabetes patients in Ghana.Methods: PubMed, PsycINFO, Scopus, and Google scholar were used to identify quantitative observational studies published between 1990 and June 2022. Studies exclusive to type 2 diabetes patients ≥18 years of age in a Ghanaian setting were included in this review. Findings of primary studies were analysed using narrative synthesis.Results: Eleven (11) studies, presenting data on a total of 2,503 type 2 diabetes patients, were included. All the studies were published in the last decade (2015-2021) and a majority (45.5%) of them were from the Greater Accra Region. The mean number of days (per week) participants adhered to a self‐care behaviour were in the ranges of 3.9–4.4 for diet, 4.2–4.8 for physical activity, 0.5–2.2 for self-monitoring of blood glucose (SMBG), and 2.9–5.0 for foot care. Adherence rates for medication were in the range of 33.5–84.5%. Patient-related factors, sociodemographic/economic-related factors, condition-related factors, and healthcare system-related factors were associated with the practice of self-care behaviours.Conclusion: Adherence to self-care behaviours among type 2 diabetes patients in Ghana remains an ongoing challenge, with significant variations in adherence among patients with different characteristics. Public health interventions are needed to address the variations in adherence to self-care behaviours among type 2 diabetes patients.
The oncogenic function and potential mechanism of basic transcription factor 3 in melanoma
Authors: Niu Ningning , Li Yongqi , Yu Hong , Guo Liang , Guo Zhen , Lan Shijie , Liu Qiongyang , Zhang Yiqun , Gui Jianping , Wu Di 
Published: August 2022

Abstract
Objective
Basic transcription factor 3 (BTF3) has been shown to exert carcinogenic effects in several types of tumors. But its role in melanoma is still unclear. This study aims to explore the proliferative effect and molecular mechanisms of BTF3 in melanoma.
Methods
We used GFP-labeled BTF3-shRNA lentiviral vectors to knock down the expression of the BTF3 gene in melanoma cells, and then evaluated the effect of BTF3 on the cell proliferation, cell cycle, apoptosis, and colony formation of melanoma cells through in vivo and in vitro experiments. Furthermore, we selected the representative tumor masses from the xenografted melanoma for microarray hybridization and bio-information analysis to screen out genes that significantly interact with BTF3. Through conducting hierarchical clustering analyze we predicted the prominent pathways and biological effects of BTF3-ralated genes, and further verify the expression of some representative genes by qRT-PCR and western blot.
Results
BTF3 is heterogeneously expressed in melanoma tissues and cells. Knockdown the expression of BTF3 attenuated the proliferation of melanoma cells both in vitro and in vivo. The melanoma cells exhibited more apoptosis, significant G2/M arrest, and deficient DNA damage repair capability conferred by transfection of BTF3-shRNA lentiviral vector. Furthermore, the results of bioinformatics analysis and western blot assay suggested that BTF3 might involve in p53 signaling, complement system, wnt/β-catenin signaling, FGF signaling, and other classical signaling pathways through interacting with some important genes such as TP53, cyclin dependent kinase inhibitor 1A (CDKN1A), checkpoint kinase 1 (CHEK1), tumor protein p53 inducible protein 3 (TP53I3), and insulin like growth factor binding protein 3 (IGFBP3). The upstream regulators of BTF3 include doxorubicin, nuclear protein 1 (NUPR1), TP53, etc.
Conclusion
BTF3 promotes the progression of melanoma by interacting with some key genes such as p53. Our findings provide novel insights into the role of BTF3 as an oncogenic gene in melanoma and suggest that BTF3 expression level may serve as a potential biomarker in response to clinical treatment.
Immune Response and Clinical Outcomes of BNT162b2 and mRNA1273 Fourth Dose COVID-19 Vaccines; Three Months Follow-up
Authors: Canetti Michal , Barda Noam , Gilboa Mayan , Indenbaum Victoria , Mandelboim Michal , Gonen Tal , Asraf Keren , Weiss-Ottolenghi Yael , Amit Sharon , Doolman Ram , Mendelson Ella , Harats Dror , Freedman Laurence S , Kreiss Yitshak , Lustig Yaniv , Regev-Yochay Gili 
Published: August 2022

Abstract
Booster doses for the ongoing COVID-19 pandemic are under consideration in many countries. We report a three-month follow-up of 700 participants in a fourth vaccine dose study, comparing BNT162b2 and mRNA1273, administered four months after a third BNT162b2 dose. Waning of the immune response was evident during follow-up, with an 11% (ß=0.89, 95% CI, 0.88–0.9) and 21% (ß=0.79, 95% CI, 0.76–0.82) multiplicative decay per week of IgG and neutralizing antibodies, respectively, in the mRNA1273 group, and of 14% (ß=0.86, 95% CI, 0.86–0.87) and 26% (ß=0.74, 95% CI, 0.72–0.76), respectively, in the BNT162b2 group. Direct neutralization of Omicron variants was low relative to ancestral strains. Cumulatively over the study period, both vaccines showed little efficacy against infection but were highly efficacious against substantial disease [89% [(IRR 0.11, 95% CI, 0.02–0.37) and 71% (IRR 0.29, 95% CI, 0.13–0.57) for mRNA1273 and BNT162b2, respectively]. These results are informative for further boosting policy-making.
Investigating the X-aminopyridine (X = 2 and 3) molecules sensing by Al12N12 and B12N12 fullerene-like nanocages: DFT, QTAIM, RDG and TD-DFT insights
Authors: Fouegue Aymard Didier Tamafo 
Published: August 2022

Abstract

The adsorption of 2-aminopyridine (2-AP) and 3-aminopyridine (3-AP) on the external surface of B12N12and Al12N12fullerene-like nanocages (FLNs) is probed herein via DFT/M06-2X/6-311G(d,p) level of theory. It came out from the study that all FLN@X-AP states investigated are spontaneously formed. Moreover, topological analysis demonstrated that the boron nitride FLN can strongly adsorbed the APs through B-N covalent interactions. Energy values of -345.22 and − 328.55 kJ/mol respectively after the adsorption of 2-AP and 3-AP were further found for the above mentioned bonds. A significant change in the HOMO-LUMO band gap of B12N12, with values of 22.01 and 32.71% have been obtained following the adsorption of 2-AP and 3-AP respectively. Accordingly, the conductivity of B12N12is greatly enhanced by the adsorption of the APs. The above observations, combined with those found from the analysis of dipole moments and molecular electrostatic potential maps predict B12N12to be more sensitive to the aminopyridines investigated than the Al12N12FLN from the theoretical point of view.
L-Arginine-containing mesoporous silica nanoparticles embedded in dental adhesive for targeting cariogenic dental biofilm
Authors: López-Ruiz Marta , Navas Francisco , Fernández-García Paloma , Martínez-Erro Samuel , Fuentes Mª Victoria , Giráldez Isabel , Ceballos Laura , Ferrer-Luque Carmen Mª , Ruiz-Linares Matilde , Morales Victoria , Sanz Raúl , García-Muñoz Rafael A. 
Published: August 2022

Abstract
Dental caries is the major biofilm-mediated oral disease in the world. The main treatment to restore the caries lesions consist of the use of adhesive resin composites due to their good properties. However, the progressive degradation of the adhesive in the medium term makes possible the proliferation of cariogenic bacteria allowing secondary caries to emerge. In this study, a dental adhesive incorporating a drug delivery system based on L-arginine-containing mesoporous silica nanoparticles (MSNs) was used to release this essential amino acid as a source of basicity to neutralise the harmful acidic conditions that mediate in the development of dental secondary caries. The in vitro and bacteria culture experiments proved that L-arginine was released in a sustained way from MSNs and diffused out from the dental adhesive, and effectively contributing to the reduction of the bacterial strains Streptococcus mutans and Lactobacillus casei. Furthermore, the mechanical and bonding properties of the dental adhesive did not change significantly after the incorporation of L-arginine-containing MSNs. These results are yielding glimmers of promise for the cost-effective prevention of secondary caries.
Developing Knowledge-Based System for Predominant Chicken Diseases Diagnosis, Prevention and Management
Authors: Girma Diriba , Jimma Worku , Diriba Chala 
Published: August 2022

Abstract
Poultry diseases remain one of the major threats to poultry production worldwide, and chicken diseases have become one of the problems that are very detrimental to chicken farmers. Therefore, it is vital to give due attention because of its impact on the health and quality of chicken production. To solve the problem, a knowledge-based system can be a powerful tool with vast potential in alleviating agricultural and medical problems. This study aims to develop a knowledge base system for diagnosing, preventing, and managing predominant chicken diseases. The Design science research method was used to develop the prototype system. The acquired knowledge was modelled and represented using a decision tree and production rules (If-Then-Action). A backward chaining algorithm was used in this study. The prototype system was tested and evaluated, including both performance of the prototype system and user acceptance. The performance comparison parameters such as precision, recall, and F-measure were used to measure the accuracy of the prototype system, and the performance was 83%. The proposed system can assist the experts in identifying the type of disease based on the chickens' symptoms. This study is significant in providing system-based help for identifying predominant chicken diseases based on their symptoms using different techniques.
Massive Multiple user MIMO Based Enhanced Spectral Efficiency with Secure Data Transmission By Deep Learning Technique in Wireless Communication Networks
Authors: Qamar Shamimul 
Published: August 2022

Abstract
Wireless communications' spectral efficiency (SE) and throughput can be significantly increased with 5G technology. Multiple input multiple output (MIMO) technology, which makes use of large antennas and user equipment(UE), has emerged as most significant technology in this respect. However, the 5G wireless technology's use of MIMO would result in higher circuit power consumption as well as lower energy efficiency (EE). This research proposed novel technique in enhancing the wireless communication network spectral efficiency with secure data transmission. here the spectral efficiency of the network has been enhanced using massive multiple user based multiple input- multiple output (MMU-MIMO). Data transmission of network has been carried out based on orthogonal frequency division multiplexing (OFDM). Base station essentially serves several users simultaneously utilising the same frequency in MMU-MIMO. The spectral performance of downlink MMU-MIMO is the subject under discussion in this work. Secure data transmission of the network has been carried out using Federated deep Q- neural networks. Our simulation findings show how, for various QoS constraint levels, the ideal number of scheduled users varies