bluuebunny/crossref_metadata_2025_split huggingface dataset:
Budget Impact Analysis of the Subcutaneous Infliximab (CT-P13 SC) for Treating Inflammatory Bowel Disease in the EU-5 Countries
Authors: Yoo Hyun Kyeong , Byun Han Geul , Caprioli Flavio , Fumery Mathurin , Peyrin-Biroulet Laurent , Subramanian Sreedhar , Potter James , Jang Minyoung 
Published: February 2022

Abstract
Background: In 2020, the European Medicines Agency approved CT-P13 subcutaneous (SC) for the treatment of inflammatory bowel disease. This new mode of infliximab administration will reduce outpatient visits and costs of intravenous (IV) administration. This article describes a budget impact analysis of introducing CT-P13 SC to the EU-5 market for 5 years, from the healthcare payer’s perspective.Methods: A prevalence-based budget impact model was developed to examine the financial impact of CT-P13 SC. “World with” versus “world without” CT-P13 SC scenarios were compared, including the potential administration costs of IV administration.Results: Introducing CT-P13 SC in patients with Crohn’s disease (CD) for 5 years resulted in cost savings of €42.0 million in the UK, €59.4 million in Germany, and €46.4 million in France and Italy, but increased budget expenditure in Spain by €3.8 million. For ulcerative colitis (UC), cost savings of €42.7 million in the UK, €44.9 million in Germany, €44.3 million in France, and €53.0 million in Italy occurred, but with no savings in Spain for 5 years. Cost-savings per patient was calculated by diving the net budget saving by number of treatment eligible patients. Maximum and minimum saving per patient per year ranged between €38.25 and €575.74 in CD, both from Germany, and €105.06 (France) and €647.25 (Germany) in UC.Conclusion: Healthcare payers in the UK, Germany, France, and Italy, but not in Spain, will make budget savings by using CT-P13 SC for the treatment of inflammatory bowel disease.
Electron spin, nutation, and wave-particle duality
Authors: Light Gregory 
Published: February 2022

Abstract
We present a diagonal spacetime 4-manifold to model the wave-particle duality with two derivations of the energy ratio of wave to particle to be 1 to 3, by which we calculate the least time for an electron nutation as based on its quadrupole spin states in a 720-degree rotation of its associated electromagnetic wave in a model of Zitterbewegung.
Combined analysis of preoperative and postoperative lymphocyte-C-reactive protein ratio precisely predicts outcomes of patients with gastric cancer
Authors: Miyatani Kozo , Sawata Shohei , Makinoya Masahiro , Miyauchi Wataru , Shimizu Shota , Shishido Yuji , Matsunaga Tomoyuki , Yamamoto Manabu , Tokuyasu Naruo , Takano Shuichi , Sakamoto Teruhisa , Hasegawa Toshimichi , Saito Hiroaki , Fujiwara Yoshiyuki 
Published: February 2022

Abstract
Background： The systemic inflammatory response resulting from the complex interactions between cancer and the host plays an important role in cancer development. Recently, the lymphocyte-C-reactive protein ratio (LCR), which is a hematological and biochemical marker that reflects the systemic inflammatory response and nutritional status, has been reported to be associated with poor survival. Similar results were observed in patients with certain cancer types. However, these studies focused on the preoperative LCR, and thus far, no studies have reported the relationship between postoperative LCR and prognosis in patients with gastric cancer (GC).Methods： This study enrolled 455 patients with a histopathological diagnosis of gastric adenocarcinoma who underwent curative surgery at our institution between 2005 and 2018. The relationship between both the preoperative and postoperative LCR and the prognosis of patients with GC was retrospectively investigated.Results： Preoperative LCR showed significant correlations with tumor-related factors, such as tumor size, depth of invasion, and lymph node metastasis. By contrast, no correlation was observed between postoperative LCR and tumor-related factors. The 5 year survival rate was significantly worse in patients with low preoperative LCR than in those with high preoperative LCR (65.4% vs. 83.9%, p < 0.0001). Similarly, the 5 year survival rate was also significantly worse in patients with low postoperative LCR than in those with high postoperative LCR (67.0% vs. 84.1%, p < 0.0001). Furthermore, combination analysis of the preoperative and postoperative LCR revealed that the prognosis of patients with both low preoperative and postoperative LCR was worse in patients with GC (5 year survival rate was 52.0%). A multivariate analysis indicated that a low preoperative and postoperative LCR and age and lymph node metastasis were independent prognostic indicators.Conclusions： The combination of preoperative and postoperative LCR appears to be useful in predicting the prognosis of patients with GC.
Epigenetic Regulations, Motif and Pathway Identification of Gabaergic Neurotransmitter’s Chip Sequence
Authors: IA Shylesh Murthy , Bagchi Preenon 
Published: February 2022

Abstract
The next generation techniques has revolutionalized the biology era. In this work we are using Chromatin immunoprecipitation (ChiP) technique on the ChIP–seq of Gamma-Amino Butyric Acid (GABA). GABA is implicated in several neurological disorders like alzheimers, rett symdrome, schizophrenia, etc. In this work, we have retrieved its chip-seq from SRA database, removed the PCR duplicates and finally identified the genome enriched region using the MAC2 call peak tool. Peak table was annotated to identify the genes and the corresponding pathways was identified from the KEGG pathway. This annotated peak table was used to identify the motifs present in the chip-seq of GABA.
Serum and Urine Uromodulin Levels in Patients Who Had Computed Tomography with Iodized Contrast Agent
Authors: Çankaya Erdem , Karaman Adem , Çelik Muhammet , DOĞAN Hasan , Uyanık Abdullah , Sevinç Can , Albayrak Bülent , Güler Muhammet Akif , Altunok Murat 
Published: February 2022

Abstract
BackgroundUromodulin is a protein produced in kidney tubule cells and found in the urine. Uromodulin levels detected in serum and urine provide information about renal tubule functions and reserve. MethodsIn this study, in individuals with normal kidney functions; the short-term changes in uromodulin levels in both urine and serum were evaluated before and after contrast agent administration 86 patients were included in the study. Serum and urine uromodulin levels were measured before and 24 hours after contrast agent administration. ResultsAlthough there was no significant change in glomerulifiltration rate before and after contrast agent administration (98.7±17.5, 100.8±18.5, p=0.1, respectively), serum uromodulin values decreased significantly (43.4±17.6, 24.8±17.9, p ≤ 0.05, respectively). Urinary uromodulin levels increased significantly (670±175, 805±340, p ≤ 0.05, respectively). ConclusionsSerum uromodulin level can be a biomarker that can be followed in ischemic kidney injury or after administration of nephrotoxic agents. Urinary uromodulin levels can provide information about renal susceptibility to nephrotoxic agents.
Seroprevalence And Risk Factors Of Coxiella Burnetii Infecting Cattle Raised In Pastoral Areas Of Narok, Kenya.
Authors: Watene Grace-Angeline , Aboge Gabriel , Gitau George , Nthiwa Daniel , Bett Bernard 
Published: February 2022

Abstract
Background: There are many prevalence and risk factor studies of infectious diseases such Q fever done in Sub-Saharan Africa but in Kenya, there are limited studies on Coxiellosis. The disease affects animal productivity and the ability to support livelihoods. The under-diagnosis of the disease has proven a challenge for effective control and eradication, since the epidemiology of the disease is still not clearly understood both in the animal and human health systems due to limited availability of technical and financial capacity to allow for significant epidemiological studies. The aim of this study was to determine the sero-prevalence and risk factors of C. burnetii in cattle in the Mara region, Narok County of Kenya. This study will provide relevant data for veterinarians and healthcare professionals that can be used for designing strategies for managing Q fever. Ultimately, this will aid in demonstrating that Q fever should not be neglected and should be included while controlling other zoonotic infections using the One Health approach. Results: A total of 589 sera samples were collected from 390 herds in 5 different villages from 3 zones selected based on the livestock-wildlife interactions and land use patterns in Mara Ecosystem in 2019. The seroprevalence C. burnetii was 1.9%. Significant risk factor on univariate analysis at animal level was bull ownership {OR=4.8 (CI 0.4-16.2) P-value 0.016}, while at at the herd level the significant factors were: animals grazing in shared areas between villages {OR 2.3CI 1.0-5.0) P-value 0.046}, mixing of cattle in different herds {OR 0.4 CI 0.2-0.9 P-value 0.029}, bought livestock previous year {OR 10.1 CI 1.3-75.8 P-value 0.025}, bull ownership P-value 0.003, history of abortion in the herd { OR 0.3 CI 0.1-10.9 P-value 0.018} and communal grazing had a borderline significance of (OR=2.2 P-Value). At the multivariable analysis bull ownership was the only significant variable {OR 5.2 CI 1.5-18.4) P-value 0.010). Conclusion: The study confirmed the previous exposure of the cattle in Mara ecosystem to the Coxiella burnetii indicating the potential risk to human transmission and qualifying the need to have better veterinary and public health interventions.
Genetic Characterization by SSR Markers of a Comprehensive Wine Grape Collection Conserved at Rancho de la Merced (Andalusia, Spain)
Authors: Cretazzo Enrico , Sanz Paula Moreno , Lorenzi Silvia , Benítez Miguel Lara , Velasco Leonardo , Emanuelli Francesco 
Published: February 2022

Abstract
Background: The IFAPA research center “Rancho de la Merced” (Jerez, Spain), hosts one of the oldest and widest grapevine germplasm banks in Europe, and is aimed at providing feasible solutions to deal with any agronomic trait by exploring its genetic variability and by means of association and DNA editing studies. In this work we focused on a wine and dual-use grapevine sub-collection that consists of 930 accessions.Results: Genetic analysis allowed to identify 521 unique genotypes. After comparing them with several databases, matches were found for 480 genetic profiles while the remaining 45 have not been previously described. Combination with clustering analysis suggested a total pool of 481 Vitis vinifera accessions that included some table cultivars. Several synonymies, homonymies and mislabeling have also been detected. Structure analysis allowed identifying six clusters according to eco-geographic cultivation areas and one additional group including non-vinifera accessions. Diversity analysis pointed out that Spanish Mediterranean varieties are genetically closer to oriental genotypes than to European varieties typical of oceanic and continental climates. The origin of Spanish varieties is discussed in depth considering our data and previous studies.Conclusions: Analysis of molecular variance partition confirmed a well-structured germplasm, although differentiation among groups had a much lower effect on genetic variability than differences within groups, which are strongly related to a very high heterozygosity. A core collection that covers all allele richness is proposed. It is constituted by about 13% of total accessions, and each cluster inferred by structure analysis is represented.
Computer Simulation And Modeling The Metal To Insulating Transition Of Liquid Mercury Via Pair, Empirical And Many-Body Potentials
Authors: Karimi Hedayat 
Published: February 2022

Abstract
In this study Monte Carlo statistical mechanics simulations were carried out to detect the metal insulator transition in of expanded liquid mercury by changing the static properties at microscopic scale. In the simulations pair, empirical, and many- body potentials governs metal–metal interactions in the canonical ensemble. Changes in structure are described in first coordination shell, while the thermodynamic properties in terms of residual internal energy, in wide of temperature range from 773 K to 1773 K. The results, reveal that the properties undergo significantly change at 1673 K, during the simulated heating process, which is in connection with the metal nonmetal transition in the liquid. The findings coincide with the experimental observation of this thermodynamics phenomenon. Notably, free energy of association that renders the system to this thermodynamic state is estimated.
Characterization Of Agrobacterium-Mediated Co-Transformation Events In Rice Using Green And Red Fluorescent Proteins
Authors: Li Lihua , Li Xin , Tian Xudan , Wang Lanlan , Zhao Jianhua , Zhou Jie , He Haiyan , Dai Liangying , Qu Shaohong 
Published: February 2022

Abstract
Biotechnologists seeking to develop marker-free transgenic plants or multigene transformants have established different co-transformation methods. To investigate the factors affecting the efficiency of co-transformation of T-DNA vectors via mixed Agrobacterium strains and to develop effective strategies for the selection of desired co-transformants, we utilized GFP and RFP as visual markers in rice co-transformation and tested the combinations of a drug-selectable vector (pGFP-HPT) and a non-drug selectable vector (pRFP) and of two drug-selectable vectors (pGFP-HPT and pRFP-HPT). Under similar conditions, the pGFP-HPT/pRFP combinations resulted in 70.9 % to 81.2 % of co-transformation frequencies while lower frequencies (56.6 % on average) were obtained in the pGFP-HPT/pRFP-HPT co-transformation. Based on the GFP/RFP segregation patterns, 45 % and 55 % of the pGFP-HPT/pRFP co-transformants contained linked and unlinked T-DNAs, respectively. Transgene transcription levels in the rice lines varied as revealed by their RT-PCR results, and tandem-linked T-DNAs were detected in most of the co-transformants, suggesting that the changed transgene expression might be related to the complicated T-DNA structures. It is thus necessary to screen the co-transformation events by evaluation of T-DNA structures and transgene expression. Therefore, the efficiency of co-transformation depends on the vector system and selection procedures and the marker-free transformation in rice can be improved by screening the gene of interest with large number of hygromycin-resistant calli. Since transgenic rice lines with desired T-DNA combinations can be selected using the GFP/RFP vector system, it is feasible to co-transform two T-DNAs carrying different plant genes for studies on gene interactions or additive effects.
The Gross Developmental Potential (GDP2): A New Approach for Measuring Human Potential and Wellbeing
Authors: Halfon Neal , Chandra Anita , Cannon Jill , Gardner William , Forrest Christopher 
Published: February 2022

Abstract
Many factors influence health and well-being of children and the adults they will become. Yet there are significant gaps in how trajectories of healthy development are measured, and the potential for leading a healthy life is evaluated. The Gross Developmental Potential (GDP2) is proposed as a new capabilities-based framework for assessing threats to thriving and understanding progress in achieving lifelong health and wellbeing. Moving beyond the GDP’s focus on economic productivity as a measure of progress, the GDP2 focuses on 7 essential inputs to lifelong health. The GDP2 capability domains include Health -living a healthy life; Needs-satisfying basic human requirements; Communication-expressing and understanding thoughts and feelings; Learning-lifelong learning; Adaption -adapting to change; Connections -connecting with others; Community -engaging in community. The project team utilized literature reviews, meetings with subject matter and technical experts, to develop the framework that was then vetted in focus groups in three diverse settings. The community leaders input resulted in refinements of the domains and how they might be applied. The GDP2 framework will next be used to develop specific measures and indices and to guide the development of community level GDP2 dashboards for local sensemaking
The deep squat test has limited clinical utility for diagnosing symptomatic femoral acetabular impingement syndrome (FAIS): A systematic review
Authors: Wood Simon , Newman Meredith , Batting Martha , Barker Karen 
Published: February 2022

Abstract
Purpose:Passive pain provocation tests (PPPT) are commonly used to diagnose FAIS but do not reflect functional restrictions. The deep squat test (DST) is a functional, weight-bearing test, but the clinical utility of the DST is unclear. Objective:To examine the validity and reliability of the DST in patients with confirmed FAISMethod:A Systematic Review registered with PROSPERO (CRD42020199249). Seven databases; CINAHL, Cochrane, Embase, Medline, PubMed, SportDiscus and Web of Science, were searched up until December 2021. Data was extracted independently, and the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool was used to evaluate the risk of bias. Result:1137 abstracts were found; 1119 were excluded after screening and 18 underwent full test review, excluding a further 17, with one meeting the search criteria. No studies reported the reliability of the DST. One study (n=78 hips) investigated criterion-based validity: pain on DST indicated a positive test and was compared with MRI and MRA measures of FAIS. QUADAS score demonstrated low risk of bias. They concluded the DST had a modest potential to identify those with FAIS, but low specificity suggested the test may yield a high number of false positives. Conclusion: Physiotherapists should use the DST with caution. Single study evidence supports its use, but despite its common use, limited information is available about the DST and further investigation is required.Impact:Further studies are needed to examine validity and reliability, including evaluating the DST with higher alpha angles to determine whether diagnostic utility is improved.
Evaluation of the Consequences of Double Ovarian Stimulation Through Shanghai Protocol in Patients With Poor Ovarian Respond
Authors: Zargar Mahvash , Pourjafar Zahra , Salemi Shabnam , Barati Mojgan 
Published: February 2022

Abstract
Purpose: This prospective study is aimed at assessing the consequences of double ovarian stimulation through Shanghai protocol in women diagnosed as poor ovarian responders (POR), compared with the conventional IVF. Methods: A total of 84 women diagnosed as POR were divided into two groups, including those who underwent conventional IVF protocol (N=42) and or Shanghai protocol (N=42). In Shanghai protocol, ovarian stimulation was firstly done in the early follicular phase and secondly in the luteal phase of the same ovarian cycle after the first oocyte retrieval. For primary ovarian stimulation, Letrozole 2.5 mg/day and Clomid 25 mg/day were administered for 4 consecutive days from cycle day 2 onwards.Results: After treatment, 1 to 5 oocytes were retrieved in 34 women (85%) underwent Shanghai protocol, which was significantly higher than its rate in the conventional IVF group [26(61.90%), p=0.02]. Subsequently, 33 women (82.50%) could develop 61 live embryos at the end of the second stimulation, which was significantly higher than its rate in another group [50%, P=0.002]. The rates of Metaphase II oocytes (60.82%) and high-quality embryos (93.44%) obtained during Shanghai protocol were significantly higher than their rates in conventional IVF (45.24% and 68.42%, respectively, p<0.05). Eventually, the clinical pregnancy success rate was significantly higher in women underwent Shanghai protocol (42.50%) than those underwent conventional IVF (19.05%) [p=0.03]. Conclusion: POR women underwent the double ovarian stimulation during Shanghai protocol-based IVF achieved the higher rates of high-quality retrieved oocytes, embryos, and successful pregnancy, compared with those who had underwent the conventional IVF.
Utility of Plasma Circulating Tumor DNA and Tumor DNA Profiles in Head and Neck Squamous Cell Carcinoma
Authors: Chikuie Nobuyuki , Urabe Yuji , Ueda Tsutomu , Hamamoto Takao , Taruya Takayuki , Kono Takashi , Yumii Kohei , Takeno Sachio 
Published: February 2022

Abstract
Early recurrence detection of head and neck squamous cell carcinoma (HNSCC) is important for improving prognosis. Recently, circulating tumor DNA (ctDNA) has been reported to be useful in early detection or treatment response determination in various carcinomas. This study aimed to identify the utility of ctDNA for predicting recurrent metastasis in patients with HNSCC. We collected pre-treatment tissues (malignant and normal tissues) and multiple plasma samples before and after treatment for 20 cases of HNSCC treated with radical therapy. ctDNA was detected in pre-treatment plasma in 10 cases; however, there were no significant associations with tumor recurrence and staging. During follow-up, ctDNA was detected in 5 of the 7 plasma samples of recurrent cases but not in the 13 recurrence-free cases. Moreover, there was a significant difference in post-treatment relapse-free survival time between the groups with and without detected ctDNA (20.6 ± 7.7 vs. 9.6 ± 9.1 months, respectively; log-rank test, p < 0.01). Moreover, for two of the five cases with ctDNA detected after treatment, ctDNA detection was a more sensitive predictor of recurrence than imaging studies. ctDNA detection during treatment follow-up was useful in patients with HNSCC for predicting the response to treatment and recurrent metastasis.
Multi-omics Revealed the Global Responses of Bovine Alveolar Macrophages to Mycobacterium tuberculosis /Mycobacterium bovis
Authors: Wei Rong , Chen Yuqi , Ma Huiya , Duan Yangbo , Ma Xueyan , Tan Lihui , Dong Jianjian , Jin Chenkai 
Published: February 2022

Abstract
The lipid composition performs important functions in interaction between macropha-ge and Mycobacterium tuberculosis (MTB)/Mycobacterium bovis (MB). Current understanding regarding the lipid responses of bovine alveolar macrophage (BAM) to MTB/MB is quite limiting. The present study conducted lipidomics and transcriptome to assess alterations in BAM lipid compositions upon MB and MTB infection. We found that both MTB and MB induced glycerophospholipids accumulation in BAM, and MTB induced more alterations in lipid composition. MTB could affect the contents of various lipids, especially ceramide phosphocholines, polystyrene(PS) (17:0/0:0), testolic acid and testosterone acetate. Meanwhile, MB particularly induced accumulation of 1-alkyl,2-acylglycerophosphoinositols. Both MB and MTB suppressed the contents of palmitoleamide, N-ethyl arachidonoyl amine, N-(1,1-dimethyl-2-hydroxy- ethyl) arachidonoyl l amine, eicosanoyl-EA, and PS (O-18:0/17:0) in BAM. Additionally, transcriptome analysis revealed that only MTB triggered genes involved in immune signaling and lipid-related pathways in BAM. And MTB mainly activated genes CXCL2 and CXCL3 relevant to NOD-like receptor, IL-17 and TNF to further induce lipid accumulation in BAM, which in turn promoted the formation of foam cells. Altogether, the results of the present study provided evidence for changes inlipid metabolism of MTB/MB-attacked BAM, and contributed towards the detection and treatment of zoonotic tuberculosis.