bluuebunny/crossref_metadata_2025_split huggingface dataset:
UX Debt in an Agile Development Process: Evidence and Characterization
Authors: Rodriguez Andres , Gardey Juan Cruz , Grigera Julián , Rossi Gustavo , Garrido Alejandra 
Published: October 2022

Abstract
The metaphor of Technical Debt (TD) has generated a conceptual framework on factors that weaken the quality of software and accumulate a repair cost. However, user-related aspects like user experience (UX) receive little consideration among TD types, for reasons like the substantial focus on code TD, some dynamics inherent to agile processes, and an apparent lack of cumulative cost over time. This article has two main goals: first, to present evidence of the existence of UXDebt as a type of TD, with a cumulative cost for the development team as well as stakeholders; second, to propose a definition and characterization of UXDebt that may serve as a frame for further research on methods and tools for continuous management within agile processes. For the first goal, we have compiled evidence on the current state of UXDebt from three sources: a literature review, a survey among software engineering professionals in agile teams, and the analysis of UX issues in GitHub. All sources have evidenced some form of UXDebt; surveyed practitioners have recognized its poor management with a cost for the entire team that accumulates over time. Moreover, issue-tracking systems allow to visualize and measure a technical form of UXDebt. For the second goal, we have defined a conceptual model that characterizes UXDebt in terms of both technical and non-technical aspects. On the technical side, we propose the notion of UX smells which allows us to discuss concrete management activities.
Relationship of cognition and Alzheimer’s disease with gastrointestinal tract disorders: a large-scale genetic overlap and Mendelian randomisation analysis
Authors: Adewuyi Emmanuel O , O’Brien Eleanor K. , Porter Tenielle , Laws Simon M 
Published: October 2022

Abstract
Background
Emerging observational evidence suggests links between cognitive impairment and a range of gastrointestinal tract (GIT) disorders; however, mechanisms underlying their relationships remain unclear. Leveraging several large-scale genome-wide association studies summary statistics, we comprehensively assessed genetic overlap and potential causality of cognitive traits and Alzheimer’s disease (AD) with GIT disorders.
Method
We assessed the global and local genetic correlation of ten cognitive traits (sample size [n] = 68,065–766,345) and AD (n = 455,258) with six GIT disorders (n = 332,601–456,327), using the linkage disequilibrium score regression (LDSC) and the Local Analysis of [co]Variant Association (LAVA) methods, respectively. We utilised the bidirectional two-sample Mendelian randomisation (MR) analysis method to investigate the potential causality between cognitive traits and GIT disorders. Also, we performed gene-based analyses and assessed the gene-level genetic overlap between cognitive traits and GIT disorders.
Results
LDSC reveals a strong and highly significant inverse global genetic correlation between all cognitive traits and GIT disorders—peptic ulcer disease (PUD), gastritis-duodenitis, diverticulosis, irritable bowel syndrome, and gastroesophageal reflux disease (GERD), but not inflammatory bowel disease (IBD). LAVA detects 35 significant (P < 4.37 × 10− 5) bivariate local genetic correlations, across 14 loci, between cognitive traits, AD and GIT disorders (including IBD). MR analysis suggests a risk-decreasing causality of educational attainment, intelligence, cognitive performance and other cognitive traits on PUD, and GERD but not IBD. Also, we found a putative causal association of GERD with cognitive function decline. These findings were supported by other MR models and sensitivity analyses. Genes with nominal association (Pgene < 0.05) were significantly enriched (Pbinomial−test = 1.18 × 10− 3 – 2.20 × 10− 16), across GIT disorders (IBD inclusive), AD, and cognitive traits—evidence of gene-level genetic overlap.
Conclusion
Our study reveals highly significant negative global and local genetic correlations of all cognitive traits with all GIT disorders, except IBD. Findings highlights causally protective roles of cognitive traits on PUD, and GERD, and a putative causal association of GERD with cognitive function decline. Identified local genetic correlations enhance novel insights, especially, into IBD’s relationship with cognitive traits and AD, characterising important targets for further investigations.
Design and development of Recirculating Aquaculture System (RAS) for sustainable production and water quality research
Authors: Potnis Atharva , Ghanekar Kshitij , Sinnarkar Atharva 
Published: October 2022

Abstract
Fisheries are the primary source of livelihood for several communities. Fishing in India is a primary industry, employing 14.5 million people and contributing to 1.07% of the total GDP of India (2020, “Fishing in India - Wikipedia). Currently, most aquaculture systems are biofloc systems or pond farming setups. While wild fishing is slowly becoming unsustainable, aquaculture faces the problem of people using incorrect filtration methods, which leads to a high mortality rate. A 3-stage filtration system for freshwater aquaculture or recirculating aquaculture system (RAS) was built to tackle this problem. The RAS prototype was designed to satisfy compactness, efficiency, longevity, and financial requirements. The culture tank (main fish tank) has a capacity of 1000 L and is stocked with 100 fish. The stocking density comes out to be almost double as compared to bio floc systems. The three stages of filtration are mechanical filtration, biological filtration, and ultraviolet (UV) filtration. The fish used to test this prototype are mono-sex tilapia(200g) (GIFT)(2019, “An Excellent Candidate Species for World Aquaculture: A Review).
Policy Reinvention in Diffusion: Evidence from Municipal Solid Waste Classification Policy in China
Authors: Zhang Qingzhi , Luo Dan , Xie Aiwen 
Published: October 2022

Abstract
While research on policy diffusion has attracted the attention of many scholars, little attention has been given to policy reinvention in diffusion. With the aim to explore the influencing factors and combination paths of policy reinvention in the Municipal Solid Waste Classification Policy (MSWCP), this study constructs a theoretical framework concerning internal factors, intergovernmental relations and external influence, and carries out an empirical analysis on the MSWCP of 31 large and medium-sized cities in China using the Qualitative Comparative Analysis. Based on the empirical results, this study identifies the different combination paths that induced local governments to reinvent policies and clarifies the various roles of financial resources, government attention, policy experience, peer competition, learning, and social demand in achieving policy reinvention, which is expected to provide new theoretical and practical enlightenment for policy reinvention research.
FTO attenuates the cytotoxicity of cisplatin on KGN cells by regulating the Hippo/YAP1 signaling pathway Short title：FTO disrupts the Hippo/YAP1 signaling pathway
Authors: Wang Rongli , Yang Xinyuan 
Published: October 2022

Abstract
Premature ovarian failure (POF) is a devastating condition for women under 40 years old, which causes serious health consequences, especially the cessation of ovarian function. They will be confronted with infertility, osteoporosis, increased cardiovascular incidence, and so on. However, its pathogenesis is not yet fully clarified. The increase in granulosa cell apoptosis is an essential pathophysiological process for POF. Our previous studies have demonstrated that FTO plays an essential role in protecting granulosa cells from cisplatin-induced cytotoxicity and down-regulation of FTO aggravated the cisplatin-induced granulosa cells apoptosis and the ovarian injury of C57BL/6 mice. Various studies have suggested that the Hippo/YAP signaling pathway plays a significant role in regulating cell apoptosis and proliferation. However, whether the Hippo/YAP signaling pathway is involved in the protective effect of FTO on granulosa cells remains to be further studied. In this study, we found that granulosa cells apoptosis increased in a concentration-dependent manner after cisplatin-treated, accompanied by the downregulation of FTO and the activation of the Hippo/YAP signaling pathway. Western blotting and qRT-PCR results indicated that overexpression of FTO promoted the expression of MST1, LATS1, YAP1, and Bcl − 2, and decreased the expression of pMST1/MST1, p-LATS1/LATS, pYAP1/YAP1, BAX, and caspase3, and the downstream targets of YAP1 (CTGF, CYR61, and ANKRD1). Immunofluorescence staining results clarified that the expression of YAP1 in the nucleus increased significantly. However, these effects were completely reversed by the small molecule inhibitor of YAP1-verteporfin (VP). Downregulation of FTO got the opposite results. Taken together, FTO may decrease injured granulosa cell apoptosis and promote its proliferation by increasing the expression of multiple genes in the Hippo/YAP signaling pathway, especially the YAP1, which serves as a significant downstream effector.
Triglyceride-glucose index as a prognostic marker after ischemic stroke or transient ischemic attack: a prospective observational study
Authors: Hoshino Takao , Mizuno Takafumi , Ishizuka Kentaro , Takahashi Shuntaro , Arai Satoko , Toi Sono , Kitagawa Kazuo 
Published: October 2022

Abstract
Background:Triglyceride-glucose (TyG) index has been proposed as a simple and creditable surrogate for insulin resistance and an independent predictor of cardiovascular outcomes. Due to lack of data on TyG index in stroke, we aimed to evaluate the predictive value of the index for recurrent vascular event risk among stroke patients.
Methods:This was a prospective observational study, in which 866 patients (mean age, 70.1 years; male, 60.9%) with ischemic stroke (n=781) or transient ischemic attack (n=85) within 1 week of onset were consecutively enrolled and followed up for 1 year. The TyG index was calculated as ln (fasting triglycerides [mg/dL] × fasting glucose [mg/dL]/2). Patients were divided into 3 groups according to the tertile of TyG index levels: tertile 1, <8.48; tertile 2, 8.48–9.01; and tertile 3, >9.01. The primary outcome was a composite of major adverse cardiovascular events (MACE), including nonfatal stroke, nonfatal acute coronary syndrome, and vascular death.
Results: The median TyG index was 8.74 (interquartile range, 8.34–9.16). Higher levels of TyG index were significantly associated with increased prevalence of ipsilateral extracranial carotid (P=0.032) and intracranial (P=0.003) atherosclerotic stenosis. There were significant differences in the MACE risk between the three groups (annual rate, 8.6%, 11.6%, and 17.3% in the tertile 1, tertile 2, tertile 3 groups, respectively; log-rank P=0.005). After multivariable adjustments, the TyG index remains to be a significant predictor of MACE, with an adjusted hazard ratio for tertile 3 versus tertile 1 groups (95% confidence interval) of 2.01 (1.16–3.47). Similar results were also found for the risk of recurrent stroke.
Conclusions: TyG index is associated with cervicocerebral atherosclerosis and the MACE risk after a stroke, suggesting the potential value of TyG index to optimize the risk stratification of stroke patients.
Trial registration:URL: https://upload.umin.ac.jp. Unique identifier: UMIN000031913.
Antibacterial and cytotoxic efficacy of Nano- Hydroxyapatite Synthesized from Eggshell and Sheep bones bio Waste
Authors: Mousavi Seyyed Mojtaba , Hashemi Seyyed Alireza , Yousefi khadijeh , Rao Neralla Vijayakameswara , Omidifar Navid , Gholami Ahmad , Ghahramani Yasmin , Chiang Wei-Hung , nezhad Fatemeh Fallahi , Kalashgrani Masoomeh Yari 
Published: October 2022

Abstract
Background:Through a new material pattern addition approach, NHA is synthesized using eggshell materials and sheep bone under different calcination temperatures (respectively at 900 ° C and 800 ° C) using a sol-gel approach. NHA/Es and NHA/Sb, due to their biocompatibility and bioactivity, are widely used in applications such as antibacterial and cytotoxic of PDL cells and teeth and as hard tissue.
Results: We investigated the physicochemical properties of NHA/Es and NHA/Sb by performing FTIR, energy scattering X-ray analysis, SEM, and XRD. The formation of NHA / Es, NHA / Sb particles occurs due to the usage of Eggshell materials and sheep bones as a pattern in NHA synthesis, which are less crystalline. Results of surface morphology analysis of NHA particles indicate that these particles are discrete and rod-shaped at low temperatures (850 °C), whereas larger particles are formed at higher temperatures. Therefore, antibacterial activity against four gram-negative bacteria were investigated through MIC and MBC methods, which included E. coli, Pseudomonas aeruginosa, Candida, Saccharomyces and gram-positive bacteria including Staphylococcus aureus and Enterococcus faecalis.
Conclusions:The reason for the compatibility of calcined NHA / Sb powder as a substance used in biomedical applications is that this powder has shown strong antibacterial efficacy against all bacterial strains with a range of inhibitory zones. The nature and structure of PDLs have been studied in many studies; While we focus on the quantitative analysis of the structural properties of PDL cells and their cytotoxic activity through the use of bio waste materials.
Identification of Cuproptosis-Related Gene in Sepsis by Machine-Learning
Authors: Xie Junjie , Xue Lijun 
Published: October 2022

Abstract
Sepsis is a serious public issue that affects millions of people. Cuproptosis, a newly form of cell death, has been linked to the course of certain illnesses. Therefore, the goal of our study was to investigate clusters of sepsis associated with cuproptosis and to develop a prediction model. Using GSE33341 dataset, the expression profiles of immune- and cuproptosis-related genes (CRGs) in sepsis patients were examined. We investigated molecular clusters based on CRGs and the accompanying immune cell infiltration. The weighted gene co-expression network analysis (WGCNA) method was used to identify differentially expressed genes in a specific cluster. Four machine learning methods were then contrasted in order to determine the best machine model. A nomogram, calibration curve, decision curve analysis were used to validate predictive efficiency. Immune infiltration analysis revealed significant immunological heterogeneity among the CRGs. The random forest machine model indicated the best discriminative performance, with comparatively smaller residual and root mean square errors and a larger area under the curve (AUC = 0.983). A final 5-gene random forest model was created, and two external validation datasets demonstrated that it performed satisfactorily (AUC = 0.964 and 0.851). The accuracy of predicting sepsis was also demonstrated by the nomogram, calibration curve, and decision curve analysis. Further investigation indicated a substantial relationship between age and SAR1B expression. This study identified the impact of cuproptosis on sepsis for the first time, and further characterized the underlying molecular pathways causing sepsis heterogeneity.
Zika Virus triggers autophagy to exploit host lipid metabolism and drive viral replication
Authors: Stoyanova Gloria , Jabeen Sidra , Vinueza Joselyn Landazuri , Roy Sounak Ghosh , Lockshin Richard , Zakeri Zahra 
Published: October 2022

Abstract
Background: Zika Virus (ZIKV), an arbovirus of global concern, has been associated with neurological complications including microcephaly in newborns and Guillain-Barré Syndrome in adults. Like other flaviviruses, ZIKV depends on cholesterol to facilitate its replication; thus, cholesterol has been proposed as a therapeutic target to treat the infection using FDA-approved statins. Cholesterol is stored in intracellular lipid droplets (LD) in the form of cholesterol esters and can be regulated by autophagy. We hypothesize that the virus hijacks autophagy machinery as an early step to increase the formation of LD and viral replication, and that interference with this pathway will limit reproduction of virus.
Methods: We pretreated MDCK cells with atorvastatin or other inhibitors of autophagy prior to infection with ZIKV. We measured viral expression by qPCR for NS1 RNA and immunofluorescence for Zika E protein.
Results: Autophagy increases in virus-infected cells as early as 6 hours post infection (hpi). In the presence of atorvastatin, LD are decreased, and cholesterol is reduced, targeting key steps in viral replication, resulting in suppression of replication of ZIKV is suppressed. Other both early- and late-acting autophagy inhibitors decrease both the number of LD and viral replication. Bafilomycin renders cholesterol is inaccessible to ZIKV. We also confirm previous reports of a bystander effect, in which neighboring uninfected cells have higher LD counts compared to infected cells.
Conclusions: We conclude that atorvastatin and inhibitors of autophagy lead to lower availability of LD, decreasing viral replication. We conclude that bafilomycin A1 inhibits viral expression by blocking cholesterol esterification to form LD.
Posterior inference on Quanto option pricing model with liquidity-adjustment
Authors: Gao Rui , Bai Yanfei 
Published: October 2022

Abstract
This paper proposes an alternative approach to estimate parameters and evaluate the quanto option with stock liquidity under Bayesian framework. First, we derive an explicit expression of the quanto option price with liquidity-adjustment in an alternative way. Then, an applicable likelihood is proposed to conduct posterior inference on model parameters based on the joint distribution of underlying stock price dynamics and the exchange rate process, which provides a new perspective to estimate the correlation coefficient. Moreover, the statistical inference on the quanto option price is conducted by the posterior distribution and numerical algorithm. The proposed method considers the impact of parameter uncertainty on option prices, particularly the correlation coefficient randomness. Finally, the numerical analysis is performed for examining the effectiveness of the proposed method in terms of estimating parameters and pricing quanto options. Empirical results indicate that the proposed method is feasible in pricing and predicting quanto option with liquidity-adjustment.
A family study and literature review of ELOVL4-associated spinocerebellar ataxia type 34
Authors: Nishide Masahiro , Marquand Kathleen Le , Davis Mark , Halmágyi Gábor M. , Fellner Avi , Narayanan Ramesh K. , Kennerson Marina L. , Reddel Stephen W. , Worgan Lisa , Kumar Kishore R. 
Published: October 2022

Abstract
Autosomal dominant disease-causing variants in the ELOVL4 gene (Elongation of Very Long Chain Fatty Acids-like 4) cause spinocerebellar ataxia type 34 (SCA34; ATX-ELOVL4), classically associated with a skin condition known as erythrokeratoderma. Here, we report a large Italian-Australian family with spinocerebellar ataxia. Notably, while there were dermatological manifestations (eczema), erythrokeratoderma was not present. Using a next generation sequencing panel, we identified a previously reported ELOVL4 variant, NM_022726.4: c.698C>T (p.Thr233Met). The variant was initially classified as a variant of uncertain significance, however, through segregation studies, we reclassified the variant as likely pathogenic. We subsequently performed the first dedicated literature review of ELOVL4 variants causing ataxia to gain further insights into genotype-phenotype relationships. We identified a total 59 reported cases of SCA34 to date. The majority had gait ataxia (88.1%), limb ataxia (76.3%), dysarthria (62.7%), and nystagmus (57.6%). Of note, skin lesions related to erythrokeratoderma were seen in a minority of cases (33.9%). Other extracerebellar manifestations included pyramidal tract signs, autonomic disturbances, retinitis pigmentosa and cognitive impairment. For brain MRI data, cerebellar atrophy was seen in all cases (100%), whereas the hot cross bun sign (typically associated with multiple-system atrophy type C) was seen in 30.3% of cases. Our family study and literature review highlight the variable phenotypic spectrum of SCA34. Importantly, it shows that erythrokeratoderma is not found in most cases and that, while a dermatological assessment may be helpful in these patients, SCA34 diagnosis should be considered irrespective of dermatological manifestations.
Primary ovarian insufficiency associated with lenvatinib therapy in a patient with hepatocellular carcinoma
Authors: Aoki Yoichi , Inoue Yosuke , Sasahira Naoki , Ono Makiko , Inamura Kentaro , Kataoka Akemi , Takano Toshimi , Kanao Hiroyuki , Watanabe Masayuki 
Published: October 2022

Abstract
Background: In recent years, the number of chemotherapy options for malignant tumors has increased due to the development of new drugs. In particular, the therapeutic effects of molecular-targeted drugs are, in some cases, more pronounced than those of conventional chemotherapy, and their introduction as a standard treatment is increasing. Due to the improved life prognosis, there are an increasing number of opportunities to consider fertility preservation for young patients. We report a case of ovarian insufficiency in a young woman caused by the tyrosine kinase inhibitor lenvatinib.
Case presentation: A 25-year-old woman received lenvatinib 8 mg/day for 98 days as preoperative chemotherapy for hepatocellular carcinoma. Blood testing the day before starting lenvatinib administration showed the following: luteinizing hormone (LH) 4.40 mIU/ml, follicle-stimulating hormone (FSH) 5.2 mIU/ml, estradiol (E2) 57.4 pg/ml, and age-equivalent hormone values. Amenorrhea occurred after the start of administration, and 48 days later, LH was 41.8 mIU/ml and FSH was 44 mIU/ml, values indicating a decrease in ovarian function. Hepatectomy was performed on the patient, and 49 days after the end of lenvatinib administration, blood test values improved to LH 4.5 mIU/ml, FSH 2.5 mIU/ml, and age-equivalent hormone values. Later, she began to have regular menstrual cycles once again.
Conclusions: Ovarian toxicity has not been recognized as a side effect of lenvatinib. However, amenorrhea and hypergonadotropinemia were observed in this patient after lenvatinib administration, as was primary ovarian insufficiencythought to be caused by the drug. Potential damage to ovarian function may need to be considered when molecular-targeted drugs with the same mechanism of action as lenvatinib are used in young women.
Quantum Gates Implementation for Reinforcement Learning Decision- Making Process
Authors: Maroufian Aarash 
Published: October 2022

Abstract
This paper introduces a novel method to improve the decision-making process of reinforcement learning agents via quantum information technology methods. In this approach, states |s〉 of the system are replaced by quantum states (eigenfunctions) of the system |ψ〉, in which a system can be in a superposition of states, and rewards of each step are calculated based on the calculated eigenvalues of the previous step. The agent decides the next step of the system based on the result of the quantum gate’s effect on the available options. Due to the high-speed, escalated performance of quantum algorithms, this method will improve the performance of reinforcement learning agents in unknown environments.