bluuebunny/crossref_metadata_2025_split huggingface dataset:
HepT1-derived murine models of high-risk hepatoblastoma display vascular invasion, metastasis, and circulating tumor cells
Authors: Woodfield Sarah E. , Mistretta Brandon J. , Patel Roma H. , Ibarra Aryana M. , Fisher Kevin E. , Sarabia Stephen F. , Gandhi Ilavarasi , Reuther Jacquelyn , Starosolski Zbigniew , Badachhape Andrew , Epps Jessica , Zorman Barry , Shah Aayushi P. , Larson Samuel R. , Srivastava Rohit K. , Shi Yan , Espinoza Andres F. , Govindu Saiabhiroop R. , Whitlock Richard S. , Holloway Kimberly , Roy Angshumoy , Sumazin Pavel , Ghaghada Ketan B. , Lopez-Terrada Dolores , Gunaratne Preethi H. , Vasudevan Sanjeev A. 
Published: April 2022

ABSTRACT
Hepatoblastoma (HB) is the most common pediatric primary liver malignancy, and survival for high-risk disease approaches 50%. Mouse models of HB fail to recapitulate hallmarks of high-risk disease. The aim of this work was to generate murine models that show high-risk features including multifocal tumors, vascular invasion, metastasis, and circulating tumor cells (CTCs). HepT1 cells were injected into the livers or tail veins of mice, and tumor growth was monitored with magnetic resonance and bioluminescent imaging. Blood was analyzed with fluorescence-activated cell sorting to identify CTCs. Intra- and extra-hepatic tumor samples were harvested for immunohistochemistry and RNA and DNA sequencing. Cell lines were grown from tumor samples and profiled with RNA sequencing. With intrahepatic injection of HepT1 cells, 100% of animals grew liver tumors and showed vascular invasion, metastasis, and CTCs. Mutation profiling revealed genetic alterations in seven cancer-related genes, while transcriptomic analyses showed changes in gene expression with cells that invade vessels. Tail vein injection of HepT1 cells resulted in multifocal, metastatic disease. These unique models will facilitate further meaningful studies of high-risk HB.
This article has an associated First Person interview with the first author of the paper.
Antibodies targeting the European lobster (Palinurus elephas) vitellogenin developed by mRNA isolation and in-silico-designed antigenic peptides
Authors: Cannea Faustina B. , Follesa Maria Cristina , Porcu Cristina , Rossino Rossano , Olianas Alessandra , Rescigno Antonio , Padiglia Alessandra 
Published: April 2022

ABSTRACT
Vitellogenin is an essential protein involved in ovary maturation in many animals. Detection of this protein correlated with reproductive capacity may be important if carried out on marine organisms such as the red spiny lobster Palinurus elephas, a crustacean that is an economically important crop from wild fish catches. Moreover, in recent years, vitellogenin has assumed an important role as a possible biomarker of marine environmental pollution, as its expression levels can be influenced by the presence of similar estrogen pollutants and can affect the reproductive sphere of marine organisms such as crustaceans. The P. elephas vitellogenin protein and its coding gene have never been isolated, so there is little information about its presence in this lobster. The aim of the present study was to develop a molecular strategy to create, for the first time, an antibody for the detection and quantization of vitellogenin in P. elephas.
A SHort course Accelerated RadiatiON therapy (SHARON) dose-escalation trial in older adults head and neck non-melanoma skin cancer
Authors: Ferro Milena , Deodato Francesco , Ferro Marica , Panza Giulia , Buwenge Milly , Pezzulla Donato , Cilla Savino , Boccardi Mariangela , Romano Carmela , Arcelli Alessandra , Cammelli Silvia , Zamagni Alice , Morganti Alessio Giuseppe , Macchia Gabriella 
Published: April 2022

Objectives:
To assess feasibility and safety of a SHort-course Accelerated RadiatiON therapy (SHARON) regimen, in the treatment of non-melanoma skin cancers (NMSC) in older patients.

Methods:
Old patients (age ≥ 80 years) with histological confirmed non-melanoma skin cancers were enrolled. The primary endpoint was to determine the maximum tolerated dose (MTD). Radiotherapy regimen was based on the delivery of four radiotherapy fractions (5 Gy per fraction) with a twice daily fractionation in two consecutive days. Three different level of dose were administered: 20 Gy (one cycle), 40 Gy (two cycles) and 60 Gy (three cycles).

Results:
Thirty patients (median age: 91 years; range: 80-96) were included in this analysis. Among fourteen patients who completed the one cycle, only one (7%) experimented acute G4 skin toxicity. Twelve patients reported an improvement or resolution of baseline symptoms (overall palliative response rate: 85.8%). Nine and seven patients underwent to two and three RT cycles, respectively: of these, no G3 toxicities were recorded. The overall response rate was 100% when three cycles were delivered. The overall six-month symptom-free survival was 78.7% and 77.8% in patients treated with one course and more courses, respectively.

Conclusions:
Short-course accelerated radiotherapy in older patients with non-melanoma skin cancers is well tolerated. High doses seem to be more effective in terms of response rate.

Advances in knowledge:
This approach could represent an option for older adults with NMSC, being both palliative (one course) or potentially curative (more courses) in the aim, accordingly to the patient’s condition.
Effectiveness of pedometer-based exercise program in phase 1 and phase 2 cardiac rehabilitation: A Systematic Review
Authors: Vasavi Vanamala Lakshmi , Khandekar Janhavi , Singh Vijay Pratap , Rajan Samuel Stephen , Cynthia D’souza Molly 
Published: April 2022

Background: Physical Inactivity (PI) and sedentary behaviour are the most important and modifiable risk factors to prevent cardiovascular diseases (CVD). Cardiac rehabilitation (CR) is a multidisciplinary program for patients who have undergone cardiac surgeries. Through CR, monitoring physical activity (PA) is possible and may reduce the rate of rehospitalization. The pedometer is a device that is useful to track the step count of the person day to day. It helps in self-monitoring of PA. The purpose of this review was to summarise the evidence about the effect of pedometer-based exercise program in phase 1 and phase 2 CR. Methods: Databases such as MEDLINE, Cochrane, Scopus, Embase, and Web of Science were searched. This search is limited to randomized controlled trials (RCTs), human trials, a 10-year period, and English language journals. Based on inclusion criteria of pedometer-based exercise programs in phase 1 (inpatient phase) and phase 2 (outpatient phase) of CR and exclusion criteria of studies using pedometer in maintenance phase CR. The primary outcome of the study is step count, while secondary is PA (in terms of time), heart rate, sedentary behaviour, and quality of life. This study quality was assessed by the Downs and Black’s checklist. Results: The study includes 6 RCTs based on the inclusion and exclusion criteria. Most studies show a considerable increase in step count associated with increased patient PA. Few studies have explained a significant increase of PA in the study group and maintained for longer terms. More research is needed to determine the impact of cardiovascular risk factors.
Assessment of serum testosterone, estradiol, and progesterone levels in female patients with acne vulgaris
Authors: Nguyen Phu Cuong , Nguyen Hoang Van , Vu Van Tien , Pham Van Tran , Tran Dang Quyet , Nguyen Thanh Tung 
Published: April 2022

Introduction: Acne is a chronic inflammatory disorder of the pilosebaceous unit with differential pathogenesis. To elucidate the roles of hormones in acne pathogenesis, we conducted a study to evaluate the serum testosterone, estradiol, and progesterone levels in women with acne vulgaris. Methods: We conducted a cross-sectional descriptive study, and 175 women with acne vulgaris were examined; their serum estradiol, progesterone, and testosterone were analyzed by chemiluminescence technique and compared with the healthy control group. Results: Increased serum hormone levels in women with acne vulgaris accounted for 29.7%, and hyperandrogenism accounted for 16.0% of cases. We found significant differences in testosterone levels (mean value, 55.67±25.56 versus 38.37±10.16 ng/dL, p<0.05) respectively, in the acne group and the control group. However, the estradiol level of the acne group (323.15±93.31 pmol/L) was lower than the control group (370.94±58.88 pmol/L), with p-value under 0.05. No statistically significant differences were found for progesterone (0.60±0.38 versus 0.50±0.15 ng/mL, p>0.05) levels. Moreover, we did not find the relationship between serum hormone levels and the severity of acne vulgaris. Conclusion: This study showed that female acne vulgaris patients may have high serum testosterone levels and low serum estradiol levels compared with those of female controls. However, hormone alterations had no correlation with acne severity.
Real-time detection method for bulk bubbles in optics based on deep learning
Authors: Wang Yue , Cheng Xinglei , Qian Changde , Zhang JianPu , Hu Xiaobo , Wang Hongxia , Huang Menghui , Lu An , Sun Huanyu , Wang Shiling , Shen Zheqiang , Wu Lan , Liu Dong 
Published: April 2022

The existence of bulk bubbles could decrease the laser-induced damage
threshold of optics and affect the beam quality, so the detection of
bulk bubbles is an essential step for quality assurance. Currently,
the inspection of bubbles in optics relies on manual work, which is
not recommended because of the low precision and inconsistency. To
improve the quality evaluation process, a real-time detection method
for bubbles inside the optics based on deep learning is proposed. Our
method can implement bubble detection at 67 fps with a recall of
0.836. As for retrieval of the radius, it costs 58.8 ms on each
bubble, and the absolute deviation is 3.73% on average. Our method
conducts real-time and accurate detection of the positions and radii
of the bubbles in the optics, thus, having significant potential for
the manufacturing process.
Presenting a comprehensive multi-scale evaluation framework for participatory modelling programs: A scoping review
Authors: Lee Grace Yeeun , Hickie Ian Bernard , Occhipinti Jo-An , Song Yun Ju Christine , Skinner Adam , Camacho Salvador , Lawson Kenny , Hilber Adriane Martin , Freebairn Louise 
Published: April 2022

IntroductionSystems modelling and simulation can improve understanding of complex systems to support decision making, better managing system challenges. Advances in technology have facilitated accessibility of modelling by diverse stakeholders, allowing them to engage with and contribute to the development of systems models (participatory modelling). However, despite its increasing applications across a range of disciplines, there is a growing need to improve evaluation efforts to effectively report on the quality, importance, and value of participatory modelling. This paper aims to identify and assess evaluation frameworks, criteria, and/or processes, as well as to synthesize the findings into a comprehensive multi-scale framework for participatory modelling programs.Materials and methodsA scoping review approach was utilized, which involved a systematic literature search via Scopus in consultation with experts to identify and appraise records that described an evaluation framework, criteria, and/or process in the context of participatory modelling. This scoping review is registered with the Open Science Framework.ResultsThe review identified 11 studies, which varied in evaluation purposes, terminologies, levels of examination, and time points. The review of studies highlighted areas of overlap and opportunities for further development, which prompted the development of a comprehensive multi-scale evaluation framework to assess participatory modelling programs across disciplines and systems modelling methods. The framework consists of four categories (Feasibility,Value,Change/Action,Sustainability) with 30 evaluation criteria, broken down across project-, individual-, group- and system-level impacts.Discussion & conclusionThe presented novel framework brings together a significant knowledge base into a flexible, cross-sectoral evaluation effort that considers the whole participatory modelling process. Developed through the rigorous synthesis of multidisciplinary expertise from existing studies, the application of the framework can provide the opportunity to understand practical future implications such as which aspects are particularly important for policy decisions, community learning, and the ongoing improvement of participatory modelling methods.
Portal vein thrombosis is associated with an increased risk of bone fractures
Authors: Gairing Simon Johannes , Galle Peter Robert , Schattenberg Jörn Markus , Kostev Karel , Labenz Christian 
Published: April 2022

Background
Portal vein thrombosis (PVT) is a rare but severe disease that often leads to portal hypertension-related complications. It is well-known that patients with portal hypertension associated with liver cirrhosis are at increased risk for bone fractures, however data on the impact of PVT on fracture risk are lacking.

Aims
This study aimed to explore the impact of PVT on the incidence of bone fractures in a large German primary care cohort.

Methods
Patients with PVT were extensively matched to non-PVT individuals in a 1:5 ratio. The primary outcome of the study was the incidence of any bone fracture.

Results
This study included 596 patients with PVT and 2,980 non-PVT individuals. During five years of follow-up, the cumulative incidence of bone fractures was significantly higher in PVT patients (n = 87, 13.6%) than in those without PVT (n = 186, 6.7%) (p<0.001). In Cox-regression analyses, PVT was positively associated with bone fractures (HR: 2.16; 95% CI: 1.59–2.93). This association was stronger in women (HR: 2.55; 95% CI: 1.65–3.95) than in men (HR: 1.87; 95% CI: 1.22–2.87). The strongest association was observed in the age group 51–60 years (HR: 2.50, 95% CI: 1.40–4.47). The association between PVT and bone fractures was maintained in subgroup analyses of patients with (HR: 2.03, 95% CI: 1.13–3.63) and without liver cirrhosis (HR: 1.82, 95% CI: 1.28–2.58).

Conclusions
PVT is independently associated with a higher incidence of bone fractures. Patients with PVT should be critically evaluated for fracture risk and preventive measures should be considered.
Fahrsituationsabhängiger Wechsel der Kommunikationstopologie für heterogene Platoons
Authors: Miekautsch Fritz , Fay Alexander 
Published: April 2022

Zusammenfassung
Kommunikationsbasierte Regler für Platooning sind oft für eine homogene Fahrzeugzusammensetzung in einer nominalen Fahrsituation ausgelegt. In realen Anwendungen werden Platoons jedoch aus unterschiedlichen Fahrzeugen bestehen und somit heterogen sein, was die Anforderungen an einen sicheren Betrieb erhöht. Darüber hinaus sind Regler, die für den nominalen Fahrzustand ausgelegt sind, nicht unbedingt in der Lage, externe Störungen zu bewältigen. In dieser Arbeit wird ein Konzept zum Wechsel der Kommunikationstopologie in Abhängigkeit von der aktuellen Fahrsituation in einem heterogenen Platoon vorgestellt. Angewandt wird das Konzept auf den kooperativen Nothalt. Für diese Situation wird gezeigt, wie eine geeignete Kommunikationstopologie gefunden werden kann und dass durch den Wechsel der Topologie Kollisionen innerhalb des Platoons vermieden werden können.
Physicochemical and bioactive properties of avocado (Persea americana Mill. cv. Lorena)
Authors: Bonilla-Loaiza Adriana Marcela , Váquiro-Herrera Henry Alexander , Solanilla-Duque José Fernando 
Published: April 2022

Abstract
Chemical compounds are of great importance in the food, cosmetic and pharmaceutical industries. Nutritional components, the presence of secondary metabolites with antioxidant and antimicrobial activity, and physicochemical properties of pulp, seed and peel of Lorena avocado cultivars were studied. Antioxidant activity was evaluated by ABTS, DPPH and lipid peroxidation in pulp, seed and peel. 26 extracts was evaluated. The results confirmed that the fruit parts stabilize free radicals and inhibit lipid oxidation processes, with the highest values in seed and peel, due to their content of flavonoids, o-diphenols and phenols. The results of phytochemical screening, antioxidant capacity and antimicrobial activity, showed significant bioprospective advantage for the presence of flavonoids, condensed tannins and total phenols in the seed, peel and pulp of Lorena avocado cultivars. Avocado fruits are rich in bioactive compounds that can be used in functional food applications.
Identifying the fourth generation of human rights in digital era
Authors: Song Lijue , Ma Changshan 
Published: April 2022

Abstract
With prominence of the typical features of digital era, particularly that people’s activities and social lives are becoming more digitalized, and humans have developed a new digital identity, the presentation and regulation of digital identity becomes an emerging theme. Based on these features of digital era, a slew of challenges have arisen, including the protection of personal privacy, preventing algorithmic bias, and balancing the imbalance between the right of public acquisition and data controlled by a few. Consequently, protecting digital rights should be embedded in public policies, to better balance rights and interests among various stakeholders. Establishing dual protection mechanisms for public and private law is therefore fitting and proper, and the future legislation may target the scenario-based protection of personal rights.
Death from the Perspective of Luhmann’s System Theory
Authors: Sariyar Murat 
Published: April 2022

Abstract
The aim of this article is to address the topic of death from a Luhmannian perspective. First, the article will introduce the general theory of Luhmann to provide a background for the way he is tackling sociological and philosophical problems and then will describe its application to religion and deduce various implications for the topic of death. For the discussion of death, we will refer to some of Hegel’s insights, as they motivated central parts of Luhmann’s theory, though he replaced the Hegelian notions with system theoretical ones. Even if it might seem like a further abstraction and mechanization, we assume that it significantly facilitates the combination of outside and inside perspectives on death. In contrast to philosophical existentialism, the system-oriented approach of Luhmann does not emphasize the situated character of human reason and its gaining authenticity by facing death and finitude. Instead, it points to the entanglement of society and consciousness, focusing on the former while providing hints to the otherness of consciousness. Here, authenticity is not achieved by writing about existential topics, but rather through some sort of parallax view.
Efficacy of Biobased Polyurethane on Bitumen Self-Healing
Authors: Kazemi Mohammadjavad , Goli Ahmad , Mohammadi Abbas 
Published: April 2022

Abstract
With inspiration from the healing of wound or fractures in living species, producing self-healable material has been investigated in the past decades. As the main component of the asphalt mixture used for road pavement, bitumen is prone to cracking under load cycles and environmental conditions. However, bitumen has the ability to heal its cracks over time inherently. Intensifying the inherent self-healing property of bitumen can significantly reduce road pavement disasters, increase users’ safety, and save a tremendous amount of money needed for the rehabilitation of pavement. To improve the self-healing of bitumen, neat bitumen was modified by polyurethanes with healable polymer networks. Also, to improve the compatibility of polyurethane with bitumen and enhance its self-healability, castor oil was used as a biomodifier in the synthesis of polyurethane. Different dosages of synthesized polyurethane produced from toluene diisocyanate and castor oil (TDI-CO) were introduced to bitumen; then the self-healing of modified blends was compared with neat bitumen using fracture-based tests at different temperatures and ages. Fourier transform infrared spectroscopy showed proper in situ polymerization of biobased polyurethane in bitumen. It also showed a reduction in the aging of bitumen by lowering carbonyl and sulfoxide (known products of aging in bitumen) by 72 % and 50 % after long-term aging. Inherent healing and ductility tests indicated the efficiency of polyurethane modification by taking advantage of regeneration of hydrogen bonds in biomodified polyurethane, reaching 100 % healing after 30 min. It confirmed that an increase in temperature improved self-healing nonlinearly, and aging deteriorated it. Owing to the supramolecular structure of polyurethane, TDI-CO showed considerable improvement in bitumen healing even at low temperatures.
A Comparative Study of LASSO and ANN Regressions for the Prediction of the Direct Tensile Behavior of UHPFRC
Authors: Abellán-García Joaquín , Ortega-Guzmán Juan J. , Chaparro-Ruiz Diego A. , García-Castaño Eliana 
Published: April 2022

AbstractDirect tensile behavior is one of the most relevant properties of ultrahigh-performance fiber-reinforced concrete (UHPFRC). However, the determination of this behavior implies the realization of complex tests that must be carried out by experienced personnel because small variations could invalidate the results. This research purpose was to develop and compare two different algorithmic approaches for the direct tensile behavior of UHPFRC. Thereby, the cracking tensile strength σcc, the tensile postcracking capacity σpc and its associated strains, εcc, and εpc, were modeled by means of least absolute shrinkage and selection operator (LASSO) regression and artificial neural networks (ANN). Four ANN models, one for each response, and four LASSO models were created to produce the most accurate approaches. Results showed the reliability of the developed models through statistical indices such as the root of the mean squared error (RMSE), mean absolute error, normalized mean bias error, the ratio of the RMSE to the standard deviation of measured data, coefficient of efficiency, and coefficient of determination (R2). Besides, the analytical research also showed that the highest accuracy belongs to ANN models, with R2 values of .922, .807, .901, and .858, in forecasting the features of direct tensile behavior of UHPFRC (σcc, εcc, σpc, and εpc).