bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neonatal and pediatric transfusion practices and policies in India: A survey‐based cross‐sectional assessment of blood centers
Authors: Arora Satyam , Dua Seema , Goel Ruchika 
Published: March 2022

AbstractBackgroundBlood centers play a critical role in supporting neonatal and pediatric transfusions. We aim to study the variation in pediatric/neonatal transfusion policies and practices for blood centers in India.Study Design and MethodsThis is a survey‐based (45 question) cross‐sectional assessment of blood centers in India supporting pediatric/neonatal transfusions.ResultsOne hundred three centers responded to the survey (response rate 51.2%; 103/201). As a part of pretransfusion testing, use of “microtainers” was reported by 58.4% (59/101) centers and only 57.4% (58/101) centers provide antibody screening. In case of absence of maternal sample, type O negative blood is most commonly used (48.5%; 49/101), and in case of ABO incompatibility, 68.3% (69/101) use units compatible with both mother and newborn. Leukoreduced RBCs are provided by 89% (90/101) centers and irradiated products are provided by 48.5% (49/101). 82% (83/101) of centers reported not receiving requests for CMV negative units considering the high incidence of CMV‐seropositivity in donors. Fresh units are defined as <7 days old by 44.5% (45/101) and <5 days old by 31.6% (32/101) centers. 97% centers reported SAGM suspended RBCs in inventory but only 67.3% (68/101) use them for top‐up transfusions to neonates. Overall >80% centers use the buffy‐coat method for platelet concentrates preparation. Volume‐based (ml/kg) aliquots preparation is done by 88% (89/101), mainly using the sterile connecting device (84.6%; 83/98).ConclusionThis survey shows heterogeneity and lack of standardization in practices across blood centers in India and highlights the need for more studies to establish best practices for this vulnerable age group.
Core complex fault rocks of the Silurian to Devonian Keisarhjelmen detachment in NW Spitsbergen
Authors: Maher* Harmon , Braathen* Alvar , Ganerød* Morgan , Osmundsen* Per Terje , Redfield* Tim , Myhre* Per Inge , Serck* Christopher , Parcher* Sara 
Published: March 2022

ABSTRACT
A Silurian–Devonian metamorphic core complex has recently been recognized in northwest Spitsbergen, on the northwest corner of the Barents Shelf at the junction between the Atlantic and Arctic oceans. The associated Keisarhjelmen detachment, a major, ductile-brittle fault zone, is 200–500 m thick and has a map trace &gt;150 km. A top-to-the-north transport direction is parallel to the axis of a large-scale, shallowly north-plunging, detachment corrugation. This detachment zone separates overlying faulted Silurian–Devonian aged cover strata from underlying migmatitic rocks in the core. The detachment shows a diverse array of fault and metamorphic rocks with structural ascent, ranging from sheared migmatite, mylonite, ultramylonite, foliated cataclasite, pseudotachylite, and breccia. Footwall post-kinematic granitic intrusions occurred shortly prior to, and likely during, deposition of the older cover strata. Variably deformed, syn-kinematic granitic sheets and veins within the detachment zone are considered coeval. Thin sections show significant grain size reduction, porphyroclasts, and well-developed composite fault surfaces. Relict garnet sigma porphyroclasts associated with chlorite and sericite indicate retrogression. Feldspar porphyroclasts show significant sericite alteration, undulose extinction and limited recrystallization low in the detachment, and brittle deformation throughout. Quartz deformation textures and grain size vary considerably within and between samples. Deformation during retrogression continued into the brittle realm with the development of thick foliated cataclasites, fault breccias, and local pseudotachylites concentrated at the top of the detachment. Biotite in particular shows grain size reduction, concentration along C-surfaces, and shredding and redistribution, suggesting it played a significant role in both ductile and brittle faulting. Veins, micro-veins, and fluid inclusion planes are ubiquitous throughout the detachment, indicating substantial fault-related fluid flow. Given existing geochronologic and P-T (pressure-temperature) data from the basement rocks of the area, the kinematics, retrogression, and ductile-brittle transition are consistent with exhumation of a core complex developing by orogen-parallel extension associated with transtension during the Late Silurian and Early to Middle Devonian in northwest Spitsbergen. Remaining questions include how this core complex connects with coeval plate-scale strike-slip faults in Svalbard, and its relationship to mainland Norwegian core complexes and Devonian basins to the south.
Background polygenic risk modulates the association between glaucoma and cardiopulmonary diseases and measures: an analysis from the UK Biobank
Authors: Kolli Ajay , Sekimitsu Sayuri , Wang Jiali , Segre Ayellet , Friedman David , Elze Tobias , Pasquale Louis R , Wiggs Janey , Zebardast Nazlee 
Published: March 2022

AimsTo assess whether associations of cardiopulmonary conditions and markers with glaucoma differ by background genetic risk for primary open angle glaucoma (POAG).MethodsWe constructed a POAG polygenic risk score (PRS) using genome-wide association study summary statistics from a large cross-ancestry meta-analysis. History of glaucoma (including self-report and codes for POAG, ‘other glaucoma’ or unspecified glaucoma), history of common cardiopulmonary conditions and cardiopulmonary measures were assessed in the UK Biobank. Stratifying by PRS decile 1 (lowest risk) versus decile 10 (highest risk), separate multivariable models were estimated to assess the associations of cardiopulmonary diseases or factors with glaucoma, adjusting for age, sex, smoking and medication use. A Bonferroni correction was used to adjust p values for multiple comparisons.ResultsIndividuals in POAG PRS decile 1 (417 cases, 44 458 controls; mean age 56.8 years) and decile 10 (2135 cases, 42 413 controls; mean age 56.7 years) were included. Within decile 1, glaucoma cases had significantly higher glycated haemoglobin (38.5 vs 35.9 mmol/mol) and higher prevalence of diabetes (17.5% vs 6.5%), dyslipidaemia (31.2% vs 18.3%) and chronic kidney disease (CKD) (6.7% vs 2.0%) than controls (adjusted p<0.0013 for each). Within decile 10, glaucoma was associated with higher prevalence of dyslipidaemia (27.7% vs 17.3%, p=6.9E-05). The magnitude of association between glaucoma and diabetes, CKD and glycated haemoglobin differed between deciles 1 and 10 (contrast test p value for difference <0.05).ConclusionThe relations between systemic conditions and glaucoma vary by underlying genetic predisposition to POAG, with larger associations among those who developed glaucoma despite low genetic risk.
Association of computed tomography screening with lung cancer stage shift and survival in the United States: quasi-experimental study
Authors: Potter Alexandra L , Rosenstein Allison L , Kiang Mathew V , Shah Shivani A , Gaissert Henning A , Chang David C , Fintelmann Florian J , Yang Chi-Fu Jeffrey 
Published: March 2022

AbstractObjectiveTo determine the effect of the introduction of low dose computed tomography screening in 2013 on lung cancer stage shift, survival, and disparities in the stage of lung cancer diagnosed in the United States.DesignQuasi-experimental study using Joinpoint modeling, multivariable ordinal logistic regression, and multivariable Cox proportional hazards modeling.SettingUS National Cancer Database and Surveillance Epidemiology End Results program database.ParticipantsPatients aged 45-80 years diagnosed as having non-small cell lung cancer (NSCLC) between 1 January 2010 and 31 December 2018.Main outcome measuresAnnual per cent change in percentage of stage I NSCLC diagnosed among patients aged 45-54 (ineligible for screening) and 55-80 (potentially eligible for screening), median all cause survival, and incidence of NSCLC; multivariable adjusted odds ratios for year-to-year changes in likelihood of having earlier stages of disease at diagnosis and multivariable adjusted hazard ratios for changes in hazard of death before versus after introduction of screening.ResultsThe percentage of stage I NSCLC diagnosed among patients aged 55-80 did not significantly increase from 2010 to 2013 (from 27.8% to 29.4%) and then increased at 3.9% (95% confidence interval 3.0% to 4.8%) per year from 2014 to 2018 (from 30.2% to 35.5%). In multivariable adjusted analysis, the increase in the odds per year of a patient having one lung cancer stage lower at diagnosis during the time period from 2014 to 2018 was 6.2% (multivariable adjusted odds ratio 1.062, 95% confidence interval 1.048 to 1.077; P<0.001) higher than the increase in the odds per year from 2010 to 2013. Similarly, the median all cause survival of patients aged 55-80 did not significantly increase from 2010 to 2013 (from 15.8 to 18.1 months), and then increased at 11.9% (8.9% to 15.0%) per year from 2014 to 2018 (from 19.7 to 28.2 months). In multivariable adjusted analysis, the hazard of death decreased significantly faster after 2014 compared with before 2014 (P<0.001). By 2018, stage I NSCLC was the predominant diagnosis among non-Hispanic white people and people living in the highest income or best educated regions. Non-white people and those living in lower income or less educated regions remained more likely to have stage IV disease at diagnosis. Increases in the detection of early stage disease in the US from 2014 to 2018 led to an estimated 10 100 averted deaths.ConclusionsA recent stage shift toward stage I NSCLC coincides with improved survival and the introduction of lung cancer screening. Non-white patients and those living in areas of greater deprivation had lower rates of stage I disease identified, highlighting the need for efforts to increase access to screening in the US.
A plasma metabolite score of three eicosanoids predicts incident type 2 diabetes: a prospective study in three independent cohorts
Authors: Tuomisto Karolina , Palmu Joonatan , Long Tao , Watrous Jeramie D , Mercader Kysha , Lagerborg Kim A , Andres Allen , Salmi Marko , Jalkanen Sirpa , Vasan Ramachandran S , Inouye Michael , Havulinna Aki S , Tuomilehto Jaakko , Jousilahti Pekka , Niiranen Teemu J , Cheng Susan , Jain Mohit , Salomaa Veikko 
Published: March 2022

IntroductionPeptide markers of inflammation have been associated with the development of type 2 diabetes. The role of upstream, lipid-derived mediators of inflammation such as eicosanoids, remains less clear. The aim of this study was to examine whether eicosanoids are associated with incident type 2 diabetes.Research design & methodsIn the FINRISK (Finnish Cardiovascular Risk Study) 2002 study, a population-based sample of Finnish men and women aged 25–74 years, we used directed, non-targeted liquid chromatography-mass spectrometry to identify 545 eicosanoids and related oxylipins in the participants’ plasma samples (n=8292). We used multivariable-adjusted Cox regression to examine associations between eicosanoids and incident type 2 diabetes. The significant independent findings were replicated in the Framingham Heart Study (FHS, n=2886) and DIetary, Lifestyle and Genetic determinants of Obesity and Metabolic syndrome (DILGOM) 2007 (n=3905). Together, these three cohorts had 1070 cases of incident type 2 diabetes.ResultsIn the FINRISK 2002 cohort, 76 eicosanoids were associated individually with incident type 2 diabetes. We identified three eicosanoids independently associated with incident type 2 diabetes using stepwise Cox regression with forward selection and a Bonferroni-corrected inclusion threshold. A three-eicosanoid risk score produced an HR of 1.56 (95% CI 1.41 to 1.72) per 1 SD increment for risk of incident diabetes. The HR for comparing the top quartile with the lowest was 2.80 (95% CI 2.53 to 3.07). In the replication analyses, the three-eicosanoid risk score was significant in FHS (HR 1.24 (95% CI 1.10 to 1.39, p<0.001)) and directionally consistent in DILGOM (HR 1.12 (95% CI 0.99 to 1.27, p=0.07)). Meta-analysis of the three cohorts yielded a pooled HR of 1.31 (95% CI 1.05 to 1.56).ConclusionsPlasma eicosanoid profiles predict incident type 2 diabetes and the clearest signals replicate in three independent cohorts. Our findings give new information on the biology underlying type 2 diabetes and suggest opportunities for early identification of people at risk.
Incidence of atrial fibrillation in elderly patients with type 2 diabetes mellitus
Authors: Matsumoto Chisa , Ogawa Hisao , Saito Yoshihiko , Okada Sadanori , Soejima Hirofumi , Sakuma Mio , Masuda Izuru , Nakayama Masafumi , Doi Naofumi , Jinnouchi Hideaki , Waki Masako , Morimoto Takeshi 
Published: March 2022

IntroductionThe incidence of atrial fibrillation (AF), a significant risk factor for cardiovascular disease (CVD), is increasing worldwide. Type 2 diabetes mellitus (T2D) and advanced age are recognized as major risk factors for AF, but herein, we evaluated the incidence of AF in elderly patients with T2D and compared the prognosis between these patients with/without AF.Research design and methodsThe Japanese Primary Prevention of Atherosclerosis with Aspirin for Diabetes (JPAD2) study is a follow-up cohort study of the JPAD trial, a randomized controlled clinical trial initiated in 2002 in 2535 Japanese patients with T2D, to examine whether low-dose aspirin prevents CVD. After completion of that trial, we followed up the patients until 2019 and evaluated the incidence of AF. We also compared the incidence of cerebral cardiovascular events in elderly patients with T2D with/without AF.ResultsDuring the median follow-up period of 10.9 years, 132 patients developed AF (incidence rate: 5.14/1000 person-years). The adjusted HRs for cerebral cardiovascular events, stroke, coronary artery disease, heart failure, and all-cause death in elderly patients with T2D with versus without AF were 1.65 (95% CI 1.03 to 2.66), 1.54 (95% CI 0.81 to 2.93), 1.96 (95% CI 1.03 to 3.73), 5.17 (95% CI 2.46 to 10.89), and 1.82 (95% CI 1.24 to 2.67), respectively.ConclusionsAnnually, 1 in 200 elderly Japanese patients with T2D are estimated to develop AF. Because elderly patients with T2D with AF are at an elevated risk for CVD, careful follow-up of this patient subgroup is necessary.Trial registration numberNCT00110448.
Practice variation across five European paediatric emergency departments: a prospective observational study
Authors: Ropers Fabienne , Bossuyt Patrick , Maconochie Ian , Smit Frank J , Alves Claudio , Greber-Platzer Susanne , moll Henriette A , Zachariasse Joany 
Published: March 2022

ObjectivesTo compare paediatric healthcare practice variation among five European emergency departments (EDs) by analysing variability in decisions about diagnostic testing, treatment and admission.Design and populationConsecutive paediatric visits in five European EDs in four countries (Austria, Netherlands, Portugal, UK) were prospectively collected during a study period of 9–36 months (2012–2015).Primary outcome measuresPractice variation was studied for the following management measures: lab testing, imaging, administration of intravenous medication and patient disposition after assessment at the ED.AnalysisMultivariable logistic regression was used to adjust for general patient characteristics and markers of disease severity. To assess whether ED was significantly associated with management, the goodness-of-fit of regression models based on all variables with and without ED as explanatory variable was compared. Management measures were analysed across different categories of presenting complaints.ResultsData from 111 922 children were included, with a median age of 4 years (IQR 1.7–9.4). There were large differences in frequencies of Manchester Triage System (MTS) urgency and selected MTS presentational flow charts. ED was a significant covariate for management measures. The variability in management among EDs was fairly consistent across different presenting complaints after adjustment for confounders. Adjusted OR (aOR) for laboratory testing were consistently higher in one hospital while aOR for imaging were consistently higher in another hospital. Iv administration of medication and fluids and admission was significantly more likely in two other hospitals, compared with others, for most presenting complaints.ConclusionsDistinctive hospital-specific patterns in variability of management could be observed in these five paediatric EDs, which were consistent across different groups of clinical presentations. This could indicate fundamental differences in paediatric healthcare practice, influenced by differences in factors such as organisation of primary care, diagnostic facilities and available beds, professional culture and patient expectations.
Interventions for physician prescribers of opioids for chronic non-cancer pain: protocol for an overview of systematic reviews
Authors: Wennberg Erica , Windle Sarah B , Filion Kristian B , Gore Genevieve , Thombs Brett D , Kudrina Irina , Paraskevopoulos Elena , Martel Marc O , Kimmelman Jonathan , Johnson Sonia , Taylor Andrew , Eisenberg Mark J 
Published: March 2022

IntroductionInterventions targeting behaviours of physician prescribers of opioids for chronic non-cancer pain have been introduced to combat the opioid crisis. Systematic reviews have evaluated effects of specific interventions (eg, prescriber education, prescription drug monitoring programmes) on patient and population health outcomes and prescriber behaviour. Integration of findings across intervention types is needed to better understand the effects of prescriber-targeted interventions.Methods and analysisWe will conduct an overview of systematic reviews. Eligible systematic reviews will include primary studies that evaluated any intervention targeting the behaviours of physician prescribers of opioids for chronic non-cancer pain in an outpatient or mixed setting, compared with no intervention, usual practice or another active or control intervention. Eligible outcomes will pertain to the intervention effect on patient and population health or opioid prescribing behaviour. We will search MEDLINE, Embase and PsycInfo via Ovid; the Cochrane Database of Systematic Reviews and Epistemonikos from inception. We will also hand search reference lists for additional publications. Screening and data extraction will be conducted independently by two reviewers, with disagreements resolved by consensus or consultation with a third reviewer. The risk of bias of included systematic reviews will be assessed in duplicate by two reviewers using the Risk of Bias in Systematic Reviews tool. Results will be synthesised narratively by intervention type and grouped by outcome. To assist with result interpretation, outcomes will be labelled as intended or unintended according to intervention objectives, and as positive, negative, evidence of no effect or inconclusive evidence according to effect on the population (for patient and population health outcomes) or intervention objectives (for prescriber outcomes).Ethics and disseminationAs the proposed study will use published data, ethics approval is not required. Dissemination of results will be achieved through publication of a manuscript in a peer-reviewed journal and conference presentations.PROSPERO registration numberCRD42020156815.
Injuries in outdoor climbing: a retrospective single-centre cohort study at a level 1 emergency department in Switzerland
Authors: Krieger Chantal Selina , Vesa Doris-Viviana , Ziegenhorn Stephan , Exadaktylos Aristomenis Konstantinos , Klukowska-Rötzler Jolanta , Brodmann Maeder Monika 
Published: March 2022

ObjectivesOutdoor rock climbing has become popular in recent years. However, few data have been published on climbing accidents in Switzerland, even though the Swiss Alps are a major climbing resort.To analyse data on accidents related to outdoor climbing treated in the Emergency Department (ED) of University Hospital Bern, Switzerland.MethodsA retrospective database search for accidents related to outdoor climbing was conducted in the clinical reporting system E.care of the ED of University Hospital Bern for the period April 2012–December 2018.Results78 patients were treated after an accident related to outdoor climbing, which accounted for 1 per 3571 (0.028%) of all ED visits during this period. Mean age was 35.8±10.4 years. 76% of patients were male. Falls were the most common mechanism of injury (64%), followed by rock or ice falling on the climber (21%). Injuries affected multiple body regions (38%) or only the lower limbs (22%). Most injuries were fractures (68%). Mean ISS was 7.5 (1–38), and grade 3 UIAA MedCom injuries were most common (45%). 11 cases of polytrauma occurred and one fatality. 44 patients needed inpatient admission. Mean duration of inpatient stay was 7 days. Mean costs per patient were 12 283 CHF.ConclusionsAccidents related to outdoor climbing accounted for a small number of patients seen in the University ED Bern. Further research should be on a nationwide basis, with collection of specific climbing data like use of a helmet and experience of climbing to inform injury prevention strategies. This should shed further light on this topic, as would a prospective study using the International Alpine Trauma Register.
Single-cell RNA sequencing reveals intrahepatic and peripheral immune characteristics related to disease phases in HBV-infected patients
Authors: Zhang Chao , Li Jiesheng , Cheng Yongqian , Meng Fanping , Song Jin-Wen , Fan Xing , Fan Hongtao , Li Jing , Fu Yu-Long , Zhou Ming-Ju , Hu Wei , Wang Si-Yu , Fu Yuan-Jie , Zhang Ji-Yuan , Xu Ruo-Nan , Shi Ming , Hu Xueda , Zhang Zemin , Ren Xianwen , Wang Fu-Sheng 
Published: March 2022

ObjectiveA comprehensive immune landscape for HBV infection is pivotal to achieve HBV cure.DesignWe performed single-cell RNA sequencing of 2 43 000 cells from 46 paired liver and blood samples of 23 individuals, including six immune tolerant, 5 immune active (IA), 3 acute recovery (AR), 3 chronic resolved and 6 HBV-free healthy controls (HCs). Flow cytometry and histological assays were applied in a second HBV cohort for validation.ResultsBoth IA and AR were characterised by high levels of intrahepatic exhausted CD8+ T (Tex) cells. In IA, Tex cells were mainly derived from liver-resident GZMK+ effector memory T cells and self-expansion. By contrast, peripheral CX3CR1+ effector T cells and GZMK+ effector memory T cells were the main source of Tex cells in AR. In IA but not AR, significant cell–cell interactions were observed between Tex cells and regulatory CD4+ T cells, as well as between Tex and FCGR3A+ macrophages. Such interactions were potentially mediated through human leukocyte antigen class I molecules together with their receptors CANX and LILRBs, respectively, contributing to the dysfunction of antiviral immune responses. By contrast, CX3CR1+GNLY+ central memory CD8+ T cells were concurrently expanded in both liver and blood of AR, providing a potential surrogate marker for viral resolution. In clinic, intrahepatic Tex cells were positively correlated with serum alanine aminotransferase levels and histological grading scores.ConclusionOur study dissects the coordinated immune responses for different HBV infection phases and provides a rich resource for fully understanding immunopathogenesis and developing effective therapeutic strategies.