bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex differences in pulmonary and systemic vascular function at rest and during exercise in healthy older adults
Authors: Sless Ryan , Wright Stephen , Bentley Robert , Valle Felipe , Mak Susanna 
Published: September 2022

Abstract
Our aim was to conduct a sex-disaggregated analysis of pulmonary and systemic vascular function in healthy individuals both at rest and during submaximal exercise. Healthy individuals underwent right-heart catheterization at rest and during submaximal cycling. Hemodynamic data were collected in a control state and with moderate exercise. Pulmonary and systemic vascular variables including: compliance, resistance, and elastance were calculated, indexed to body surface area (BSA), adjusted for age and compared between male and female sex. Thirty-six individuals (18M/18F; 54±7 vs. 58±6 years, p=0.04) were included. When adjusted for age and indexed to BSA, total pulmonary resistance (TPulmR) (516±73 vs. 424±118 WU•m-2, p=0.03) and pulmonary arterial elastance (PEa) (0.41±0.1 vs. 0.32±0.1 mmHg•mL-1•m2, p=0.03) were higher in females vs. males. Both pulmonary (Cpa) and systemic compliance (Csa) were lower in females vs. males however lost significance with adjustment for age. Systemic arterial elastance (SEa) was higher in females (1.65±0.29 vs. 1.31±0.24 mmHg•mL-1, p=0.05). Secondary analyses demonstrated significant correlations between age and PVR (r=0.33, p=0.05), TPulmR (r=0.35, p=0.04), Cpa (r=-0.48, p<0.01), and PEa (r=0.37, p=0.03). During exercise, there were greater increases in TPulmR (p=0.02) and PEa (p=0.01) in females vs. males. TPulmR and PEa are significantly higher at rest and exercise in females vs. males. Cpa and Csa were lower in females, however this may have been confounded by age. Our results are consistent with the notion that indices of pulmonary and systemic vascular load are increased related to both older age and female sex.
Loss of INI1 inhibits the expression of SIDT1 and promotes tumor progression in skull base chordoma by regulating EZH2-mediated H3K27me3
Authors: Zhang Yazhuo , Shen Yutao , Li Mingxuan , Xiong Yujia , Ma Tianshun , Bai Jiwei , Li Chuzhong 
Published: September 2022

AbstractIntegrase interactor 1 (INI1) loss is associated with a poor prognosis of skull base chordoma, while the molecular mechanism remains generally unclear. Hence, we herein explored the regulatory mechanism underlying INI1 action in skull base chordoma. We exploited transcriptomic sequencing of 48 skull base chordomas to analyze theINI1-correlated genes, and we found thatEZH2was negatively correlated withINI1. As EZH2 regulated the expression of the repressive histone mark H3K27me3, we applied chromatin immunoprecipitation (ChIP) sequencing of H3K27me3 to investigate the downstream molecules involved. ChIP sequencing and ChIP-qPCR revealed that H3K27me3 directly bound to theSIDT1promoter, and qRT-PCR verified that H3K27me3 suppressed the transcription ofSIDT1. The expression of SIDT1 in skull base chordoma was assessed using immunohistochemical staining and its low expression was associated with a poor prognosis in chordoma patients. When the potential tumor-suppressive effects ofSIDT1 were further investigatedby cytologic experiments, our results verified that SIDT1 played a tumor-suppressive role in chordoma both in vitro and in vivo. In conclusion, these findings suggested the INI1-EZH2-H3K27me3-SIDT1 axis as a possible novel therapeutic target in skull base chordoma.
The functional impact of two variants of uncertain significance in TNFAIP3
Authors: Wang Li , Gan Rui , An Yunfei , Zhao Xiaodong , Tang Xuemei , Wu Junfeng 
Published: September 2022

Abstract
Purpose Mutations in TNFAIP3 have recently been recognized as critical cause leading to early-onset autoinflammatory and autoimmune syndrome. And gradually more TNFAIP3 gene mutations were reported, most were frameshift and truncation mutations, and only a few were missense mutations. Here, we reported five Chinese patients manifested with unclassified autoinflammatory syndrome which exhibit two identical novel missense heterozygous variants of uncertain significance (VUS) mutations in TNFAIP3 and verified their pathogenicity.
Methods We analyzed the clinical, genetic, and immunological features of five Chinese patients with two novel missense heterozygous VUS in TNFAIP3, and verified their pathogenicity.
Results We identified two missense heterozygous mutations ( c.208 G>A, p.Asp70Asn and c.770 T>C, p.Phe257Ser), which were located in the highly conserved residue of amino-terminal ovarian tumor (OTU) domain of TNFAIP3. Only the p.Asp70Asn mutation changes the structure of TNFAIP3 but both variants alter the expression of A20 in peripheral blood mononuclear cells (PBMCs). Accordingly, in vitro TNF-α stimulated patients’ PBMCs showed higher levels of p65 NF-kB phosphorylation and increased IkBα degradation, as well as increased production of the proinflammatory cytokines IL-1β, IL-18 and TNF-a after lipopolysaccharide (LPS) stimulation in vitro
Conclusion our data further expands the understanding of the HA20 disease.andhighlights the necessity of functional analysis to evaluate the pathogenicity of VUS in TNFAIP3 for accurate diagnose of HA20.
Identification of an emerging cucumber virus in Taiwan using Oxford nanopore sequencing technology
Authors: Dong Zi-Xuan , Lin Chian-Chi , Chen Yuh-Kun , Chou Chia-Cheng , Chen Tsung-Chi 
Published: September 2022

Abstract
Background
In June 2020, severe symptoms of stunting, leaf mosaic and fruit malformation were observed on greenhouse-grown cucumber plants in Xizhou Township of Changhua County, Taiwan. An unknown virus, designated CX-2, was isolated from a diseased cucumber sample by single lesion isolation on Chenopodium quinoa leaves. Identification of CX-2 was performed. Moreover, the incidence of cucumber viruses in Taiwan was also investigated.
Methods
Transmission electron microscopy was performed to examine virion morphology. The portable MinION sequencer released by Oxford Nanopore Technologies was used to detect viral sequences in dsRNA of CX-2-infected leaf tissue. The whole genome sequence of CX-2 was completed by Sanger sequencing and analyzed. Reverse transcription-polymerase chain reaction (RT-PCR) with species-specific primers and indirect enzyme-linked immunosorbent assay (ELISA) with anti-coat protein antisera were developed for virus detection in the field.
Results
Icosahedral particles about 30 nm in diameter were observed in the crud leaf sap of CX-2-infected C. quinoa plant. The complete genome sequence of CX-2 was determined as 4577 nt long and shared 97.0-97.2% of nucleotide identity with that of two cucumber Bulgarian latent virus (CBLV) isolates in Iran and Bulgaria. Therefore, CX-2 was renamed CBLV-TW. In 2020–2022 field surveys, melon yellow spot virus (MYSV) had the highest detection rate of 74.7%, followed by cucurbit chlorotic yellows virus (CCYV) (32.0%), papaya ringspot virus virus watermelon type (PRSV-W) (10.7%), squash leaf curl Philippines virus (SLCuPV) (9.3%), CBLV (8.0%) and watermelon silver mottle virus (WSMoV) (4.0%). Co-infection of CBLV and MYSV could be detected in field cucumbers.
Conclusions
The emerging CBLV-TW was identified by nanopore sequencing. Whole genome sequence analysis revealed that CBLV-TW is closely related, but phylogenetically distinct, to two known CBLV isolates in Bulgaria and Iran. Detection methods including RT-PCR and indirect ELISA have been developed to detect CBLV and to investigate cucumber viruses in central Taiwan. The 2020–2022 field survey results showed that MYSV and CCYV were the main threats to cucumbers, with CBLV, SLCuPV and WSMoV were occasionally occurring. Co-infection of CBLV and MYSV severely affected cucumber plants, suggesting a synergistic effect of CBLV and MYSV.
Genome-wide identification and analysis of glyceraldehyde-3-phosphate dehydrogenase family reveals the role of GmGAPDH14 to improve salt tolerance in soybean (Glycine max L.)
Authors: Zhao Xunchao , Hong Huilong , Wang Jie , Zhan Yuhang , Teng Weili , Li Haiyan , Li Wenbin , Li Yonguang , Zhao Xue , Han Yingpeng 
Published: September 2022

Abstract
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an essential key enzyme in glycolytic metabolic pathway, and plays an important role in plant growth and stress responses. In this study, sixteen GmGAPDH genes were identified on nine soybean chromosomes, which were classified into three clusters. Meantime, all GmGAPDH gene harbor two highly conserved domain, including Gp_dh_N (PF00044) and Gp_dh_C (PF02800) domain. The qRT-PCR analysis showed that most GmGAPDH genes were significantly responded to multiple abiotic stresses, including NaHCO 3 , PEG, Cold, Salt. Among them, GmGAPDH14, which was extraordinarily induced to salt stress. The GmGAPDH14 gene was cloned and overexpressed in yeast and soybean hair root. We found the GmGAPDH14 overexpressing yeast grew better than the control (pYES3 empty vector) under salt stress. The GmGAPDH14 gene overexpressed transgenic soybean plants also showed better growth than control plants. Moreover, the transgenic plants overexpressing GmGAPDH14 gene had higher activities of peroxidase (POD) and superoxide dismutase (SOD), and higher proline (Pro) level, but lower malonaldehyde (MDA) content than control plants under salt stress. Meantime, four haplotypes were found for GmGAPDH14 gene and haplotype 2, 3 and 4 were beneficial haplotype for soybean resistance to the salt stress. These results suggest that the GmGAPDH14 gene might be involved in the process of soybean tolerance to salt stress in soybean.
Intracranial carotid artery calcification patterns and ischemic stroke: a novel marker for stroke etiology?
Authors: Mazzacane Federico , Bello Beatrice Del , Ferrari Federica , Persico Alessandra , Rognone Elisa , Pichiecchio Anna , Padovani Alessandro , Cavallini Anna , Morotti Andrea , Arba Francesco 
Published: September 2022

Abstract
Intracranial carotid artery calcifications (ICAC) are a common finding on non-contrast computed tomography (NCCT) and have been associated with an increased risk of ischemic stroke. Intimal, medial, and mixed subtypes of ICAC have been recognized, but no data are available about the association between ICAC patterns and stroke etiology. The aim of our study was to investigate the association between ICAC patterns and etiological subtypes of ischemic stroke. We retrospectively analyzed a single center cohort of patients admitted for ischemic stroke with known etiology. Patients with a first ischemic stroke with lacunar, cardioembolic or atherothrombotic large artery disease etiology were included. Each carotid artery was evaluated separately on NCCT scans to define the ICAC pattern. The association of ICAC patterns and stroke etiology was investigated using two logistic regression models adjusting for confounders. A total of 300 patients were included in the analysis, mean age 78 years (+-10), 158 (53%) female, median NIHSS 6 (IQR 9). Prevalence of medial, intimal, and mixed calcification was: 73%, 17% and 11% in cardioembolic stroke, 66%, 25% and 9% in large artery atherosclerosis stroke and 37%, 44% and 19% in lacunar stroke (p < 0.001). After adjustment for confounders, intimal pattern (OR = 2.14; 95%CI = 1.07–4.30), and medial pattern (OR = 0.33; 95CI%=0.16–0.67) remained independently associated with lacunar stroke etiology. In conclusion, our study suggests that intimal ICAC pattern is associated with lacunar stroke, and, if validated in further studies, it may serve as a marker for lacunar stroke etiology.
MVM-LBP: Mean−Variance−Median based LBP for face recognition
Authors: Arora Nitin , Sucharitha G. , Sharma S C 
Published: September 2022

Abstract
This paper proposes a novel descriptor called Mean-Variance-Median based LBP (MVM-LBP). The Median binary pattern (MBP) calculates the difference between median of all the pixels in a 3 × 3 pixel window and respective eight neighbours is used. The main draw back of MBP is that two different pixel windows can have same MBP code which is not appropriate in face recognition systems. To improve the performance of the system, the proposed descriptor (MVM-LBP) involves more statistics and uses the mean, variance, and median values of all the pixels in a 3 × 3 pixel window to get a more robust feature vector. To check the excellence the of the proposed descriptor, the proposed method is applied on two publicly available facial data sets AT&T, and faces94. The outcomes show that the proposed descriptor gives encouraging results.
A Cross-sectional Survey of Public Knowledge of the Monkeypox Disease in Nigeria
Authors: Al-Mustapha Ahmad Ibrahim , Sikiru Nafisah Ayinde , Kolawole Barakat , Oyewo Muftau , Ahmed Hager , Odukoya Adesoji , Ogundijo Olwaseun Adeolu , Asiegbu Emmanuella Chinenye , Nanven Magdalene B , Lawal-Atolagbe Taibat , Lawal-Lah Fatima , Awoyale Oladayo David , Abubakar Ahmed Tijani , Sahabi Kabiru , Babandi Zaharadeen Z 
Published: September 2022

Abstract
Recently named a global health emergency by the World Health Organization, the Monkeypox Disease (MPXD) has gained attention due to its widened geographical distribution outside of Africa and also due to the novel sexual transmission route. The index case in Europe was from a Nigerian traveler. This study assessed public awareness and knowledge of the MPXD by conducting an online, cross-sectional survey of educated Nigerians. The study participants were recruited using the snowball sampling method between the 16 th to 29 th of August 2022. A total of 822 Nigerians were included in this study. More responses were retrieved from the Northeastern geopolitical region (30.1%, n = 220) than other regions. Our study participants had high awareness of MPXD (89%, n = 731/822) but only 58.7% (n = 429/731) of them had good knowledge of MPXD with a mean knowledge score of 5.31 ± 2.09. The main knowledge gaps were in the incubation period of the disease, the signs and symptoms, its mode of transmission, and preventive practices needed to curb the spread of the monkeypox virus (MPXV). Specifically, only 24.48% (n = 179) knew that MPXV can be transmitted via sexual contact. Most of the study participants (79.2%, n = 651) opined that we can prevent the occurrence of public health emergencies in the future. The multivariable logistic regression analysis revealed that the male gender (OR: 1.69; 95% CI: 1.22,2.33); Ph.D. level education (1.44; 95% CI: 0.48,4.23); and being homosexual (OR:1.65; 95% CI: 0.71,3.78) were significantly associated with good knowledge of MPXD. Despite the varying prevalence across the country, the region of residence within Nigeria did not influence the knowledge of MPXD among the respondents. Despite the high educational status of the majority of our respondents, there were key gaps in the public knowledge of the MPXD in Nigeria. Therefore, public health risk communication must be intensified with a focus on modes of transmission and the preventive measures needed to curb the spread of the MPXV using an integrated one-health approach.
A Personalized Short Video Recommendation Method Based on Multimodal Feature Fusion
Authors: Li Hui , Lin Jingchen , Wang Tai , Zhang Li , Wang Peng 
Published: September 2022

Abstract
In order to achieve accurate parsing of multiple elements of short videos, multimodal short video processing with text, image, and speech as the three elements needs to be realized. Specifically, text information is the external marker data presented by short videos, and text information retrieval can currently be achieved by crawler crawling technology. Image information is the frame data extracted from the short video at certain time intervals by means of frame processing, and there are many types of image formats. The frame structure and pixel composition of different types of image files are completely different. Voice information is the stereo background music present in short videos, and this music voice also has different formats. Different voice formats also require different technical processing. Therefore, for short video recommendation techniques, the processing results of the above three text, image, and voice information need to be fully understood and analyzed comprehensively in order to come up with the best recommendation results. In this paper, based on multimodal content analysis techniques, we propose a framework for integrated processing of the three elements of information of short videos to form a unified information representation and to realize the elemental processing of the three elements of multimodal short videos at the semantic level. Multimodal content analysis plays an important role in short video recommendation applications. In this paper, a multimodal data fusion short video recommendation method is proposed, in which the key issue is how to effectively combine information from different modalities in order to provide recommendations to users with target information. The method not only considers user behavior features and short video label features, but also maps video from high-dimensional space to low-dimensional dense space, and extract the short video word vector features. At the same time, we consider that there is a relationship between the user's clicking behavior and the short video cover image and profile. The short video image features and text features are fused with the structured features and trained together to complete the short video recommendation task. The method makes full use of the differences and similarities between different models to increase the model's understanding of user behavior and improve the short video recommendation.
Preparation and characterization of polyethyleneimine functionalized magnetic graphene oxide as high uptake and fast removal for Hg (II)
Authors: Wang Deqi , Ge Huacai 
Published: September 2022

Abstract
Polyethyleneimine functionalized magnetic graphene oxide adsorbent (PEI-mGO) was synthesized by introducing polyethyleneimine onto Fe3O4/graphene oxide. The structures and morphologies of PEI-mGO was identified by using Fourier-tranform infrared (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM) methods. Quantities of bar-like Fe3O4 nanoparticles were observed on the surfaces of PEI-mGO. The adsorption of PEI-mGO for Cu(II), Pb(II), Hg(II), Co(II) and Cd(II) was compared. The adsorption results indicated that PEI-mGO showed higher uptake for Hg(II) than the other ions. The influence of various variables for the adsorption of Hg(II) on PEI-mGO was explored. The adsorption kinetics and isotherm could be described well by the pseudo-second-order and Langmuir models. The maximal uptake of PEI-mGO for Hg(II) from Langmuir model was 857.3 mg g−1, which was higher than that reported previously. The adsorption removal was a fast and endothermic process governed by the chemical process. The uptake increased with increasing temperature. PEI-mGO showed an excellent performance for removal of Hg(II) with 93.3% removal efficiency from simulated wastewater. Adsorption-desorption cycled experiments indicated that PEI-mGO could be recycled. PEI-mGO could be easily separated from the adsorbed solution by using a magnet. Hence, this novel adsorbent would be promising for the removal of Hg(II) from wastewater.
INTEGRASI PENDIDIKAN KARAKTER DALAM PEMBELAJARAN MATA KULIAH KOMUNIKASI INTERPERSONAL
Authors: Aw. Suranto 
Published: September 2022

Abstrak: Penelitian ini bertujuan untuk mengetahui apakah integrasi pendidikan karakter dalam pembelajaran mata kuliah Komunikasi Interpersonal dapat meningkatkan pemahaman terhadap nilai-nilai karakter mulia yang mencakup sopan-santun, keterbukaan, empati, dan kesetaraan. Subjek penelitian adalah mahasiswa semester VI Prodi Pendidikan Administrasi Perkantoran yang mengambil mata kuliah Komunikasi Interpersonal. Penelitian ini merupakan penelitian tindakan kelas. Penelitian ini berlangsung dalam dua siklus. Langkah-langkah penelitian meliputi: perencanaan, implementasi, monitoring, dan refleksi. Data penelitian diperoleh melalui: (1) observasi; (2) dokumentasi untuk mengumpulkan dokumen hasil pekerjaan atau tugas mahasiswa; dan (3) kuesioner untuk mahasiswa. Analisis data dalam penelitian ini berupa refleksi terhadap data yang telah diperoleh, baik secara kualitatif maupun kuantitatif. Hasil penelitian menunjukkan bahwa melalui integrasi pendidikan karakter dalam mata kuliah tersebut, pemahaman mahasiswa terhadap nilai-nilai karakter mulia mengalami peningkatan. Implementasi tindakan dapat dikatakan berhasil karena telah terjadi perubahan dan peningkatan dalam pemahaman terhadap nilai karakter mulia yang mencakup sopan-santun, keterbukaan, empati, dan kesetaraan. Kata Kunci: integrasi, pendidikan karakter, komunikasi interpersonal THE INTEGRATION OF CHARACTER EDUCATION IN THE TEACHING AND LEARNING OF INTERPERSONAL COMMUNICATION CLASS Abstract: This research aims at finding out whether integrating character education in the teaching and learning of Interpersonal Communication class can promote the understanding of noble character values which include politeness, openness, empathy, and equality. The subjects of the research were Semester VI students of Office of Administration Education Study Program taking the Interpersonal Communication class. This is an action research study lasting within two cycles. The research steps include planning, implementation, monitoring, and reflection. The data were obtained through (1) observation, (2) documentation – collecting documents pertaining to the students’ works and tasks, and (3) quesionnaire for the students. The data analysis in this study took the form of reflection on the qualitative and quantative data obtained. The result of the study shows that, through the integration of character education in the Interpersonal Communication class, the students’ understanding of the noble character values improved. The implementation of the action is considered successful because there have been changes and improvement in the students’ understanding of the noble character values which include politeness, openness, empathy, and equality. Keywords: integration, character education, interpersonal communication