bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA‐9a‐5p inhibits mucosal barrier injury in inflammatory bowel disease
Authors: Zhou Xiaohong , Wang Jin , Li Wenyan , Zhang Caiqun , Gu Yanling 
Published: June 2022

AbstractOur previous research found that FOXO1 aggravates the mucosal barrier injury in inflammatory bowel disease (IBD) by regulating TLR4/MD2 signaling. In this study, we further reveal the mechanism of action whereby miRNA‐9a‐5p inhibits the mucosal barrier injury after regulating FOXO1. An IBD model was established in C57BL/6N mice using dextran sulfate sodium (DSS). The effects of endogenous miRNA‐9a‐5p were mimicked/antagonized by intraperitoneally injecting miRNA‐9a‐5p agomir and antagomir. Body weights of mice were monitored and the disease activity index scores were assessed. H&E staining was performed to examine pathological changes, while immunohistochemical (IHC) staining was conducted to measure the expressions of TJ proteins (ZO‐1, Occludin), as well as FOXO1 and TLR4. The mucosal permeability was assessed by FITC‐D, the tissue inflammatory cytokines were detected by enzyme linked immunosorbent assay, and the expressions of ZO‐1 and Occludin were measured through Western blot analysis. Caco‐2 cells were cultured in vitro to establish a monolayer model of the mucosal barrier. TNF‐α was used to induce the cell damage, while agomir and antagomir were transfected to mimic/antagonize the miRNA‐9a‐5p action, followed by determination of barrier permeability. There was a targeted regulatory relationship between MiRNA‐9a‐5p and FOXO1. MiRNA‐9a‐5p could suppress the FOXO1 expression, thereby downregulating the TLR4 signaling activation, inhibiting the mucosal barrier injury, and elevating the expressions of TJ proteins. We also found in Caco‐2 cells that miRNA‐9a‐5p could protect cells from inflammatory injury and reduce permeability. In rescue experiments, the effect of agomir was found inhibited by the overexpression of FOXO1 in agomir‐treated cells. This study found that miRNA‐9a‐5p could inhibit the TLR4 signaling activation by targeting FOXO1, thereby exerting a protective effect on the mucosal barrier injury in IBD.
Preparation, DFT calculations, docking studies, antioxidant, and anticancer properties of new pyrazole and pyridine derivatives
Authors: Zebbiche Zineddine , Şekerci Güldeniz , Boulebd Houssem , Küçükbay Fatümetüzzehra , Tekin Suat , Tekin Zehra , Küçükbay Hasan , Sandal Süleyman , Boumoud Boudjemaa 
Published: June 2022

AbstractSeven novel pyrazole derivatives (4a–g) and four novel starting compounds incorporating substituted pyridine moieties were synthesized successfully. Cell viability assay for the tested compounds was performed, and the inhibitory concentrationlogarithmic 50 (LogIC50) values of the compounds were calculated after a 24‐h treatment. Four of the examined compounds (3d, 3g, 4f, and 4g) showed comparable cytotoxic activity against CaCo‐2 compared to the standard drug docetaxel at 0.1 and 1 μM concentrations. Although the LogIC50 of docetaxel was −0.678 μM for CaCo‐2 cells at 24 h, the LogIC50 values of compounds were −0.794, −0.567, −0.657, and −0.498 μM, respectively. Five of the compounds (2d, 2g, 3d, 3g, and 4e) showed comparable cytotoxic activity against MCF‐7 at 0.1 μM concentration compared to docetaxel (p < 0.05). Docking studies revealed the compounds have a good affinity to the active site of the human topoisomerase II β enzyme. The antioxidant capacities of all compounds were determined using both 1,1‐diphenyl‐2‐picrylhydrazyl and metal chelation methods. Although the compounds did not show significant antioxidant activity, relatively effective are compounds 3c, 3d, and 3g, which are hydrazine derivatives with approximately 50% antioxidant activity of standard antioxidants at concentrations of 62.5 and 125 μg/ml.
Characteristics of hepatocellular carcinoma in patients with hepatitis C virus who received direct‐acting antiviral therapy and achieved sustained virological response: The impact of a hepatologist on surveillance
Authors: Tada Toshifumi , Kumada Takashi , Matono Tomomitsu , Nakamura Shinichiro , Sue Masahiko , Matsuo Yu , Takatani Masahiro , Iijima Hiroko , Tanaka Junko 
Published: June 2022

AbstractBackground and AimThe relationship between the characteristics of hepatocellular carcinoma (HCC) diagnosed after sustained virological response (SVR) with direct‐acting antiviral (DAA) therapy and surveillance status has not been sufficiently investigated. This study investigated the clinical risk factors for HCC development and HCC characteristics according to which type of physician performed follow‐up after SVR.MethodsA total of 1070 patients in whom hepatitis C virus (HCV) was eradicated with DAA therapy were evaluated.ResultsThere were 458 patients followed by hepatologists (specialist group) and 612 followed by non‐hepatologists (non‐specialist group) after SVR. During the follow‐up period, 54 patients developed HCC. The 1‐, 2‐, 3‐, 4‐, and 5‐year cumulative incidence rates of HCC were 1.8, 4.1, 6.9, 10.5, and 17.2%, respectively. Multivariate Cox proportional hazards analysis showed that male sex (hazard ratio [HR], 3.139; 95% confidence interval [CI], 1.732–5.690), α‐fetoprotein level (HR, 1.056; 95% CI, 1.035–1.077), and fibrosis‐4 (FIB‐4) index (HR, 1.051; 95% CI, 1.017–1.085) were significantly associated with HCC development, while the follow‐up physician type after SVR was not. There were 25 patients with stage I HCC, 17 with stage II, 9 with stage III, and 3 with stage IV. Multivariate ordinal logistic regression showed that follow‐up physician type (non‐specialist) (HR, 39.100; 95% CI, 9.350–224.00) was independently associated with HCC stage, while α‐fetoprotein level and FIB‐4 index were not.ConclusionWhen patients have more risk factors for HCC development after SVR (i.e., male sex, elevated α‐fetoprotein, or elevated FIB‐4 index), they should be followed by a hepatologist for HCC surveillance.
Variations in the carapace shape of whip spiders (Arachnida: Amblypygi)
Authors: Réveillion Florian , Montuire Sophie , Maquart Pierre‐Olivier , Fétiveau Charlotte , Bollache Loïc 
Published: June 2022

AbstractMorphological studies often need to reference body size to correctly characterise the shape of organisms. In arthropods, the most commonly used reference for this is the length or width of the carapace, thorax, or the prosoma in the case of chelicerates. However, in the case of animals with unlimited growth, such as whip spiders, this measure could be irrelevant if growth is allometric. In this study, we analyse the ontogenetic modifications in prosoma outline shape in whip spiders during growth and compare the differences in shape between species. Differences are important for the relative prosoma width between species and, in the case of Damon medius, during growth in the juvenile stages, whereas the shape remains stable in mature stages. We conclude that a one‐dimensional measure (i.e., length or width) suffices for mature specimens of a single species or family, but for larger studies, or when including immature specimens, at least the prosoma area (within the outline shape) should be used as a size estimator.
Amylase as a Diagnostic Tool for Plunging Ranula: Clinical Series and Description of the Technique
Authors: Song Thomas , Chiu Weldon , de Paiva Leite Sandro , Ahmad Zahoor , Mahadevan Murali , Harrison John D. , Jain Prabha , Morton Randall P 
Published: June 2022

ObjectivesThis study describes a technique of measurement for neck cyst amylase content and reviews the experience of a tertiary referral center for cases of suspected plunging ranula.MethodsA retrospective study was performed at the Manukau Surgical Center in Auckland, New Zealand. Patients with a possible diagnosis of plunging ranula based on clinical presentation and diagnostic aspiration of the cyst contents were included. Demographic data, imaging and laboratory findings were collected, along with findings from surgery and histology. The technique for measuring the amylase of the aspirated cyst contents was also carefully recorded.ResultsThe 37 cases of confirmed plunging ranula included in this study had a submandibular cystic swelling that was aspirated. Imaging features consistent with a plunging ranula were seen in 89% of the study group. All cases had detectable levels of amylase of ≥3 U/L in the ranula contents. There was large variability (range: 5–560 U/L) in the concentration of amylase, with 70% of the cases demonstrating an amylase concentration below 200 U/L. Aspirates were typically described as viscous (87.5%) and yellow or straw‐colored.ConclusionThe combination of clinical presentation, imaging and the presence of amylase in the cyst contents is diagnostic for plunging ranula.Level of Evidence4 Laryngoscope, 133:535–538, 2023
Clinical and dermoscopic evaluation of melanocytic nevi changes during diode laser hair removal: A prospective study
Authors: Acle Renata , Zambrano‐Mericq María José , Navarrete‐Dechent Cristian , Uribe Pablo , Abarzúa‐Araya Álvaro 
Published: June 2022

AbstractBackgroundLaser hair removal (LHR) is a common practice with increasing use worldwide. Clinical and dermoscopic changes in melanocytic nevi after LHR have been reported but prospective studies are lacking.ObjectiveTo describe dermoscopic changes of melanocytic nevi at different time points after LHR.MethodsProspective study in a cohort of female patients undergoing diode LHR. Dermoscopic follow‐up of at least three nevi on the legs that underwent hair removal. We included three nonexposed nevi on the arms as controls. Two blinded investigators analyzed dermoscopic images, according to variables selected based on the available literature.ResultsThirty‐four patients were included with a total of 148 nevi on the legs and 112 nevi on the arms (controls). 47.9% (71/148) of the nevi on the legs had evidence of dermoscopic changes at the sixth hair removal session, compared to 9.8% (11/112) on controls (p < 0.001). The most frequent change was “bleaching” (41.9%, 62/148). Also, we observed “irregular hyperpigmented areas,” and “regression structures” in 5.4% (8/148) and 4.7% (7/148) of the cases at the sixth session, respectively. Neither of these structures were observed in the controls (p < 0.05).LimitationsOnly females were included; we did not perform histopathological evaluation nor reflectance confocal microscopy of changing nevi.ConclusionMelanocytic nevi frequently change after diode LHR. The changes cannot always distinguish between LHR induced and melanoma, so we advise avoiding nevi during laser therapies with melanin targets.
The ethnomedicinal and functional uses, phytochemical and pharmacology of compounds from Ardisia species: An updated review
Authors: Liu Bingrui , Liu Rongyu , Liu Qifeng , Ashby Charles R. , Zhang Hang , Chen Zhe‐Sheng 
Published: June 2022

AbstractMedicinal plants are considered to be a critical source of novel compounds and pharmacophores. The genus Ardisia, consisting of approximately 500 species, is the largest genus in the Myrsinaceae family. Ardisia species are widely distributed throughout tropical and subtropical regions of the world and have been used for the treatment of cancer, hypertension, irregular menstruation, gonorrhea, diarrhea and postnatal syndromes, among others. Phytochemical studies of Ardisia species have resulted in the isolation and identification of 111 compounds, including triterpenoid saponins, quinones, phenols, coumarins, cyclic depsipepetide and flavonoids. Crude extracts and isolates from Ardisia have been reported to have in vitro and in vivo efficacies, including but not limited to anticancer, antiinflammatory, antimicrobial, antioxidant, antithrombotic and antidiabetic, antitubercular compounds. This review focuses on the medical and functional uses, phytochemical profile and pharmacological efficacies of Ardisia species over the past 15 years. This review will provide information indicating that Ardisia species represent an invaluable source of potential therapeutic compounds.
Botulinum toxin in patients at‐risk for bladder augmentation: Durable impact or kicking the can?
Authors: Bowen Diana K. , Meyer Theresa , Rosoklija Ilina , Sturm Renea , Chu David I. , Cheng Earl Y. , Yerkes Elizabeth B. 
Published: June 2022

AbstractPurposeDeferring bladder augmentation (BA) may be desirable in a pediatric neurogenic bladder (NGB) with worsening compliance, but prior studies have questioned whether onabotulinum toxin A (BTX) demonstrates durable improvement in compliance. We present our overall experience with BTX and its role in mitigating the “at‐risk” NGB, as determined by urodynamic characteristics.MethodsA retrospective single‐institution review of all BTX procedures performed during January 2010 to October 2018 was conducted. Patients with <12 months follow‐up after first BTX injection were excluded. Urodynamic studies (UDS) were reviewed to assign National Spina Bifida Patient Registry (NSBPR) as well as institutionally developed (LCH) risk classification groups. Patients were considered “at‐risk” for BA if they had any of the following: NSBPR risk grade of intermediate or hostile; LCH risk grades indicating end‐fill pressure >25 cmH2O or detrusor sphincter dyssynergia (DSD); upper tract changes including new hydronephrosis; new or worsening vesicoureteral reflux; or other signs of a hostile urinary tract (i.e., febrile UTIs). UDS risk grades pre and post‐first BTX injection were compared in this “at‐risk” group, when available.ResultsThirty‐nine patients underwent 162 injection procedures over a median follow‐up of 65 months interquartile range (IQR 49–81). Median age at first BTX was 10 years and the median number of BTX injections per patient was 4 (IQR 2–7; range 1–12) with a median time of 6 months between injections (IQR 4–10). Twenty‐six patients were deemed “at‐risk” at the time of first BTX injection, and of those, 16 (61.5%) proceeded to BA at a median of 36 months (IQR 22.5–42). A small number (four) had BA due to upper tract changes or worsening pressures on BTX, while nine patients (five with CKD) proceeded to BA given a lack of sufficient improvement to consider BTX a long‐term viable option. Post‐first BTX UDS demonstrated downgrading of risk group in 38% and 63% using NSBPR and LCH classifications, respectively.ConclusionsEncouraging improvements in the urodynamic risk group were noted in some patients. With careful counseling and follow‐up, BTX may safely extend the time to BA in some “at‐risk” patients.
Rapid profiling of potential antitumor polymethoxylated flavonoids in natural products by integrating cell biospecific extraction with neutral loss/diagnostic ion filtering‐based high‐performance liquid chromatography–quadrupole time‐of‐flight tandem mass spectrometry
Authors: Cheng Li , Wang Fang , Cao Yuanxin , Tong Chaoying , Wei Qisheng , Shi Shuyun , Guo Ying 
Published: June 2022

AbstractIntroductionCitri Reticulatae Pericarpium Viride (CRPV, Qing Pi in Chinese) has been widely used in traditional Chinese medicine. Polymethoxylated flavonoids (PMFs), which are a special group of flavonoids with strong antitumor activity, are broadly distributed in citrus peels. However, systematic investigation of antitumor PMFs in CRPV has received little attention to date.ObjectivesAn MCF‐7 cell biospecific extraction method integrated with neutral loss/diagnostic ion filtering‐based HPLC‐QTOF‐MS/MS strategy was developed for rapid and specific profiling of antitumor PMFs and systematic identification of PMFs in CRPV.MethodologyBy incubating MCF‐7 cells with CRPV extract, potential antitumor PMFs specifically bound to cells and were isolated. Then, by systematic investigation of fragmentation pathways, neutral loss and diagnostic ion filtering strategies were proposed to comprehensively and accurately identify PMFs.ResultsSixteen antitumor PMFs were unambiguously or tentatively identified. Among them, minor compound 15 (5‐hydroxy‐6,7,8,3′,4′‐pentamethoxyflavone with a free hydroxyl group at C‐5) exhibited excellent antitumor activity, with an IC50 value of 2.81 ± 0.76 μg/mL, which is lower than that of 5‐fluorouracil (IC50, 4.92 ± 0.83 μg/mL). Nobiletin (12) and tangeretin (16), two major PMFs, presented moderate antitumor activities with IC50 values of 13.06 ± 1.85 and 17.07 ± 1.18 μg/mL, respectively, and their contents were sensitively and precisely determined.ConclusionsTo the best of our knowledge, this is the first report on the systematic investigation of antitumor PMFs in CRPV. The study will lay a foundation for the quality control and clinical application of CRPV.
Solar cell efficiency tables (Version 60)
Authors: Green Martin A. , Dunlop Ewan D. , Hohl‐Ebinger Jochen , Yoshita Masahiro , Kopidakis Nikos , Bothe Karsten , Hinken David , Rauer Michael , Hao Xiaojing 
Published: June 2022

AbstractConsolidated tables showing an extensive listing of the highest independently confirmed efficiencies for solar cells and modules are presented. Guidelines for inclusion of results into these tables are outlined, and new entries since January 2022 are reviewed. An appendix describing temporary electrical contacting of large‐area solar cells approaches and terminology is also included.
Can omic tools help generate alternative newer sources of edible seed oil?
Authors: Rangan Parimalan , Maurya Rasna , Singh Shivani 
Published: June 2022

AbstractThere are three pathways for triacylglycerol (TAG) biosynthesis: De novo TAG biosynthesis, phosphatidylcholine‐derived biosynthesis, and cytosolic TAG biosynthesis. Variability in fatty acid composition is mainly associated with phosphatidylcholine‐derived TAG pathway. Mobilization of TAG‐formed through cytosolic pathway into lipid droplets is yet unknown. There are multiple regulatory checkpoints starting from acetyl‐CoA carboxylase to the lipid droplet biogenesis in TAG biosynthesis. Although a primary metabolism, only a few species synthesize oil in seeds for storage, and less than 10 species are commercially exploited. To meet out the growing demand for oil, diversifying into newer sources is the only choice left. The present review highlights the potential strategies targeting species like Azadirachta, Callophyllum, Madhuca, Moringa, Pongamia, Ricinus, and Simarouba, which are not being used for eating but are otherwise high yielding (ranging from 1.5 to 20 tons per hectare) with seeds having a high oil content (40–60%). Additionally, understanding the toxin biosynthesis in Ricinus and Simarouba would be useful in developing toxin‐free oil plants. Realization of the importance of cell cultures as “oil factories” is not too far into the future and would soon be a commercially viable option for producing oils in vitro, round the clock.
Living with or beyond lymphoma: A rapid review of the unmet needs of lymphoma survivors
Authors: Boland Vanessa , Drury Amanda , Sheaf Greg , Brady Anne‐Marie 
Published: June 2022

AbstractObjectiveTo establish an understanding of the unmet needs of people living with or beyond a lymphoma diagnosis. Survivors of lymphoma are at increased risk of unmet needs due to cancer, treatment‐related toxicities and extended survivorship. Despite the rapidly growing numbers of lymphoma survivors, their needs and research priorities are underserved and undervalued, therefore left largely unaddressed.MethodsA rapid review method and reflexive thematic analysis approach assimilated current knowledge. Eligibility criteria included quantitative, qualitative, or mixed approaches employing cross‐sectional, longitudinal, cohort or review designs focused on the needs of adult lymphoma survivors (any subtype or stage of disease). Five databases: CINAHL, EMBASE, Medline, PsycInfo and Scopus, were systematically searched.ResultsForty‐seven studies met the inclusion criteria via a stringent screening process facilitated by NVivo. Almost 60 per cent of articles were published within the last five years and investigated a homogenous lymphoma sample. Most studies employed quantitative approaches (77%) and cross‐sectional designs (67%). Studies were of high methodological quality. Five major themes were identified: disparity in health service delivery, the psychological impact of cancer, impactful and debilitating concerns, the monetary cost of survival and insufficient provision of survivorship information. A meta‐analytical approach was not feasible due to the breadth of methodologies of included studies.ConclusionsThis review shows that lymphoma survivors experience a myriad of unmet needs across multiple domains, reinforcing the need for lymphoma‐specific research. However, more research is needed to advance and achieve informed decision‐making relating to survivorship care, placing due attention to the needs and research priorities of lymphoma survivors.
Living alone in the age of freedom: The paradox of solo households in postapartheid South Africa
Authors: Posel Dorrit , Hunter Mark 
Published: June 2022

AbstractThis paper considers an apparent paradox in South Africa: the share of single‐person households increased after the end of apartheid despite the ending of apartheid's racial laws that restricted ‘African’ families from living together, the postapartheid state's building of around four‐million low‐cost houses for families, the economies of scale offered by multiple‐person households in a country where poverty remains prevalent and the reduced significance of some of the longstanding sites of single‐person households—including mining compounds and hostels for men. Bringing together quantitative and qualitative data, we argue that solo dwelling remains associated with persistent rural–urban spatial divisions, increased migration and urbanization, continued declining marriage rates and the nature of employment. These arguments are supported by quantitative data that show an increase in solo dwelling, its greater occurrence among men and working‐age African men, in particular, its concentration in certain urban spaces and the way it fluctuated in the 2000s according to changing labour market conditions. We also draw on a township survey and interviews that show how, as apartheid restrictions collapsed, new areas of land suitable for solo living became available in the 1990s and provide examples of unmarried and married adults living alone, including to access work opportunities.
A case report of coronavirus disease‐2019‐induced acute asthma exacerbation in an obese patient
Authors: Sasaki Hisashi , Miyata Jun , Kobayashi Ai , Kawano Shuichi , Kawana Akihiko 
Published: June 2022

AbstractThe coronavirus disease‐2019 (COVID‐19) has spread worldwide. Previous studies indicated a low prevalence of COVID‐19 induced acute exacerbations of asthma. We present a 39‐year‐old‐male obese asthmatic case who experienced acute asthma exacerbation during COVID‐19. On day 8 of infection, his cough and dyspnea worsened with hypoxia and wheezing. Laboratory test results revealed elevated interleukin‐6 (IL‐6) and total immunoglobulin E (IgE) levels without eosinophilia. Two months since the onset, hypoxia disappeared with decreased IL‐6 and IgE levels. Our case suggested that obesity and high serum IL‐6 and IgE levels may have contributed to atypical asthma exacerbation in COVID‐19.