bluuebunny/crossref_metadata_2025_split huggingface dataset:
Achieving large thermal hysteresis in an anthracene-based manganese(II) complex via photo-induced electron transfer
Authors: Hu Ji-Xiang , Li Qi , Zhu Hai-Lang , Gao Zhen-Ni , Zhang Qian , Liu Tao , Wang Guo-Ming 
Published: May 2022

AbstractAchieving magnetic bistability with large thermal hysteresis is still a formidable challenge in material science. Here we synthesize a series of isostructural chain complexes using 9,10-anthracene dicarboxylic acid as a photoactive component. The electron transfer photochromic Mn2+ and Zn2+ compounds with photogenerated diradicals are confirmed by structures, optical spectra, magnetic analyses, and density functional theory calculations. For the Mn2+ analog, light irradiation changes the spin topology from a single Mn2+ ion to a radical-Mn2+ single chain, further inducing magnetic bistability with a remarkably wide thermal hysteresis of 177 K. Structural analysis of light irradiated crystals at 300 and 50 K reveals that the rotation of the anthracene rings changes the Mn1–O2–C8 angle and coordination geometries of the Mn2+ center, resulting in magnetic bistability with this wide thermal hysteresis. This work provides a strategy for constructing molecular magnets with large thermal hysteresis via electron transfer photochromism.
Biophysical and functional study of CRL5Ozz, a muscle specific ubiquitin ligase complex
Authors: Campos Yvan , Nourse Amanda , Tanwar Ajay , Kalathur Ravi , Bonten Erik , d’Azzo Alessandra 
Published: May 2022

AbstractOzz, a member of the SOCS-box family of proteins, is the substrate-binding component of CRL5Ozz, a muscle-specific Cullin-RING ubiquitin ligase complex composed of Elongin B/C, Cullin 5 and Rbx1. CRL5Ozz targets for proteasomal degradation selected pools of substrates, including sarcolemma-associated β-catenin, sarcomeric MyHCemb and Alix/PDCD6IP, which all interact with the actin cytoskeleton. Ubiquitination and degradation of these substrates are required for the remodeling of the contractile sarcomeric apparatus. However, how CRL5Ozz assembles into an active E3 complex and interacts with its substrates remain unexplored. Here, we applied a baculovirus-based expression system to produce large quantities of two subcomplexes, Ozz–EloBC and Cul5–Rbx1. We show that these subcomplexes mixed in a 1:1 ratio reconstitutes a five-components CRL5Ozz monomer and dimer, but that the reconstituted complex interacts with its substrates only as monomer. The in vitro assembled CRL5Ozz complex maintains the capacity to polyubiquitinate each of its substrates, indicating that the protein production method used in these studies is well-suited to generate large amounts of a functional CRL5Ozz. Our findings highlight a mode of assembly of the CRL5Ozz that differs in presence or absence of its cognate substrates and grant further structural studies.
Reconfigurable switching between reflecting/absorbing modes in VO2 assisted graphene-coated hemispherical dielectric hole arrays
Authors: Raad Shiva Hayati 
Published: May 2022

AbstractIn this paper, a graphene-coated dielectric hole array is used to design a reconfigurable switchable optical reflector/absorber device. The design benefits from the collective excitation of localized surface plasmon resonances of graphene-coated hole array, providing simpler fabrication fellow and more compact structure with respect to graphene-coated spherical nanoparticle array with similar plasmonic behavior. Geometrical parametric study of the reflecting mode shows that the device has lots of degrees of freedom for spectrum tuning and can highly tolerate fabrication imperfections. Moreover, the reflection rate is slightly affected by the dielectric substrate height, which can be tuned to achieve strong absorption by backing it with a metallic mirror. The designed absorber efficiently captures a wide range of obliquely incident transverse electric (TE) and transverse magnetic (TM) waves. Also, the operating frequency of both reflecting and absorbing modes can be tuned after fabrication, thanks to the two-dimensional nature of graphene material. Finally, using vanadium dioxide (VO2) phase change material, the switchable reflector\absorber mode of the device is also exhibited.
Phylogeography of the veined squid, Loligo forbesii, in European waters
Authors: Göpel Anika , Oesterwind Daniel , Barrett Christopher , Cannas Rita , Caparro Luis Silva , Carbonara Pierluigi , Donnaloia Marilena , Follesa Maria Cristina , Larivain Angela , Laptikhovsky Vladimir , Lefkaditou Evgenia , Robin Jean-Paul , Santos Maria Begoña , Sobrino Ignacio , Valeiras Julio , Valls Maria , Vieira Hugo C. , Wieland Kai , Bastrop Ralf 
Published: May 2022

AbstractThe veined squid, Loligo forbesii Steenstrup, 1856, occurs at the European Shelf areas including the Azores and represents a valuable resource for the European commercial fishery in the North East Atlantic. However, very little is known about its population structure and phylogeography. This lack of knowledge also impedes the development of sustainable fishery management for this species. The present study combined the use of two types of markers that retrieve patterns of gene flow in different time spans; the analysis of 16 nuclear microsatellites and sequencing of the mitochondrial cytochrome oxidase subunit I (COI). Whereas the high mutation rate of microsatellites allows the description of recent patterns of connectivity in species, the lower mutation rate of COI provides phylogeographic patterns on a longer timescale. A total of 347 individuals of L. forbesii were investigated from nearly the entire distribution range of the species, including the North East Atlantic Shelf, the Azores and the Mediterranean. Individuals from the Western and Eastern Mediterranean Sea have never been included in a genetic study before. We were able to analyse COI sequences from all 12 sampling areas and define three clades of L. forbesii. Due to our large sampling area, we are presenting 13 COI-haplotypes that were previously unknown. The microsatellite analysis does not include the Azores but three main clades could be identified at the remaining 11 sampling sites. Low FST values indicate gene flow over large geographical distances. However, the genetically significant differences and an additional slight grouping in the microsatellite structure reveal that geographical barriers seem to influence the population structure and reduce gene flow. Furthermore, both markers provide strong evidence that the observed phylogeographic pattern reflects the geographical history of the Azores and the Mediterranean Sea.
Development and application of a field knowledge graph and search engine for pavement engineering
Authors: Yang Zhihao , Bi Yingxin , Wang Linbing , Cao Dongwei , Li Rongxu , Li Qianqian 
Published: May 2022

AbstractIntegrated, timely data about pavement structures, materials and performance information are crucial for the continuous improvement and optimization of pavement design by the engineering research community. However, at present, pavement structures, materials and performance information in China are relatively isolated and cannot be integrated and managed. This results in a waste of a large amount of effective information. One of the significant development trends of pavement engineering is to collect, analyze, and manage the knowledge assets of pavement information to realize intelligent decision-making. To address these challenges, a knowledge graph (KG) is adopted, which is a novel and effective knowledge management technology and provides an ideal technical method to realize the integration of information in pavement engineering. First, a neural network model is used based on the principle of deep learning to obtain knowledge. On this basis, the relationship between knowledge is built from siloed databases, data in textual format and networks, and the knowledge base. Second, KG-Pavement is presented, which is a flexible framework that can integrate and ingest heterogeneous pavement engineering data to generate knowledge graphs. Furthermore, the index and unique constraints on attributes for knowledge entities are proposed in KG-Pavement, which can improve the efficiency of internal retrieval in the system. Finally, a pavement information search engine based on a knowledge graph is constructed to realize information interaction and target information matching between a webpage server and graph database. This is the first successful application of knowledge graphs in pavement engineering. This will greatly promote knowledge integration and intelligent decision-making in the domain of pavement engineering.
Cognitive and hippocampal changes weeks and years after memory training
Authors: Bråthen Anne Cecilie Sjøli , Sørensen Øystein , de Lange Ann-Marie G. , Mowinckel Athanasia M. , Fjell Anders M. , Walhovd Kristine B. 
Published: May 2022

AbstractWhile immediate effects of memory-training are widely reported in young and older adults, less is known regarding training-dependent hippocampal plasticity across multiple intervention phases, and long-term maintenance of such. Here, 157 healthy young and older adults underwent a training-intervention including two 10 weeks periods of episodic-memory training, separated by two 2 weeks periods of no training. Both age groups showed improvements on a criterion task, which prevailed after 3 years. When compared to the reference condition of no training, relative increases in hippocampal volume were observed after the training across age groups, which were maintained after 10 weeks periods of no training. However, there was age-group dependent temporal variation with respect to timing of effects. Hippocampal volume of the training group did not differ from that of a passive control-group 3 years after the intervention. The young showed an immediate near-transfer effect on a word-association task. We show that training-gains on memory performance can prevail for at least 3 years. Memory training can induce increases in hippocampal volume immediately after the intervention and after months. Episodic-memory training can produce transfer effects to a non-trained memory task in young adults. However, maintained effects on hippocampal volume beyond 10 weeks are uncertain, and likely require continuous training.
The central role of self-esteem in the quality of life of patients with mental disorders
Authors: Barbalat Guillaume , Plasse Julien , Gauthier Emmanuel , Verdoux Hélène , Quiles Clélia , Dubreucq Julien , Legros-Lafarge Emilie , Jaafari Nematollah , Massoubre Catherine , Guillard-Bouhet Nathalie , Haesebaert Frédéric , Franck Nicolas 
Published: May 2022

AbstractIn psychiatry, recent years have seen a change of focus from a clinician- to a patient-centered perspective that emphasizes quality of life as a treatment target. As a complex construct, quality of life is composed of multiple dimensions that interact with one-another (e.g. physical and psychological well-being, relationships, autonomy, self-esteem). Here, we used data from the REHABase cohort, which includes N = 2180 patients from 15 psychosocial rehabilitation centers in France, to explore networks of quality-of-life dimensions among six psychiatric disorders: schizophrenia, neurodevelopmental, bipolar, depressive, anxiety, and personality disorders. Stronger connections (edges) involved the Self-Esteem dimension, such as Self-Esteem–Physical Well-Being, Self-Esteem–Autonomy, Self-Esteem–Psychological Well-Being, and Self-Esteem–Resilience. Self-esteem was also consistently retrieved as the most central node (the dimension with the most connections within each network). Between-group tests did not reveal any differences regarding network structure, overall connectivity, edge-weights, and nodes’ centrality. Despite presenting with different symptom profiles, various psychiatric disorders may demonstrate similar inter-relationships among quality-of-life dimensions. In particular, self-esteem may have a crucial inter-connecting role in patients’ quality of life. Our findings could support treatment programmes that specifically target self-esteem to improve patients’ quality of life in a cost-effective way.
Preliminary outcomes of the surgical navigation system combined with intraoperative three-dimensional C-arm computed tomography for zygomatico-orbital fracture reconstruction
Authors: Chu Yu-Ying , Yang Jia-Ruei , Lai Bo-Ru , Liao Han-Tsung 
Published: May 2022

AbstractThis study analyzed the outcomes of zygomatico-orbital fracture reconstruction using the real-time navigation system with intraoperative three-dimensional (3D) C-arm computed tomography (CT). Fifteen patients with zygomatico-orbital or isolated orbital/zygoma fractures were enrolled in this prospective cohort. For zygoma reduction, the displacement at five key sutures and the differences between preoperative and intraoperative CT images were compared. For orbital reconstruction, the bilateral orbital volume differences in the anterior, middle, and posterior angles over the medial transitional buttress were measured. Two patients required implant adjustment once after the intraoperative 3D C-arm assessment. On comparing the preoperative and postoperative findings for the zygoma, the average sum of displacement was 19.48 (range, 5.1–34.65) vs. 1.96 (0–3.95) mm (P < 0.001) and the deviation index was 13.56 (10–24.35) vs. 2.44 (0.6–4.85) (P < 0.001). For the orbit, the mean preoperative to postoperative bilateral orbital volume difference was 3.93 (0.35–10.95) vs. 1.05 (0.12–3.61) mm3 (P < 0.001). The mean difference in the bilateral angles at the transition buttress was significantly decreased postoperatively at the middle and posterior one-third. There was no significant difference in orbital volume, angle of the transition zone, and the sum of five zygoma distances between post operative results and preoperative virtual planning. The surgical navigation system with the intraoperative 3D C-arm can effectively improve the accuracy of zygomatico-orbital fracture reconstruction and decrease implant adjustment times.
Novel pfk13 polymorphisms in Plasmodium falciparum population in Ghana
Authors: Matrevi Sena Adzoa , Tandoh Kwesi Zandoh , Bruku Selassie , Opoku-Agyeman Philip , Adams Tryphena , Ennuson Nana Aba , Asare Bright , Hagan Oheneba Charles Kofi , Abuaku Benjamin , Koram Kwadwo Ansah , Fox Ann , Quashie Neils Ben , Letizia Andrew G. , Duah-Quashie Nancy Odurowah 
Published: May 2022

AbstractThe molecular determinants of Plasmodium falciparum artemisinin resistance are the single nucleotide polymorphisms in the parasite’s kelch propeller domain, pfk13. Validated and candidate markers are under surveillance in malaria endemic countries using artemisinin-based combination therapy. However, pfk13 mutations which may confer parasite artemisinin resistance in Africa remains elusive. It has therefore become imperative to report all observed pfk13 gene polymorphisms in malaria therapeutic efficacy studies for functional characterization. We herein report all novel pfk13 mutations observed only in the Ghanaian parasite population. In all, 977 archived samples from children aged 12 years and below with uncomplicated malaria from 2007 to 2017 were used. PCR/Sanger sequencing analysis revealed 78% (763/977) of the samples analyzed were wild type (WT) for pfk13 gene. Of the 214 (22%) mutants, 78 were novel mutations observed only in Ghana. The novel SNPs include R404G, P413H, N458D/H/I, C473W/S, R529I, M579T/Y, C580R/V, D584L, N585H/I, Q661G/L. Some of the mutations were sites and ecological zones specific. There was low nucleotide diversity and purifying selection at the pfk13 locus in Ghanaian parasite population. With increasing drug pressure and its consequent parasite resistance, documenting these mutations as baseline data is crucial for future molecular surveillance of P. falciparum resistance to artemisinin in Ghana.
Metabolite profiling and protein quantification to a large library of 96 horsegram (Macrotyloma uniflorum) germplasm
Authors: Gautam Manisha , Chahota Rakesh Kumar 
Published: May 2022

AbstractThe present study was framed for the assessment of metabolic diversity of 96 diverse horsegram lines derived from 700 germplasm accessions of Pan India. The nutritional component of horsegram germplasm was studied using nuclear magnetic resonance spectroscopy (1H NMR) and micro-Kjeldal method. Differential range of protein content was found 13–40% in the selected panel. The related wild species Macrotyloma sar-gharwalensis contained highest protein content (40%), and the lowest protein content (13%) was found in IC-120837 and TCR-1439. Seed based 1H NMR spectroscopy of horsegram discovered 45 different metabolites (17 amino acids, 7 flavonoids, 10 organic acids/phenolic acids/sugar alcohols/steroids, 7 carbohydrades/sugars, and 4 vitamins). The number of total metabolites quantified among diverse horsegram panel ranged from 25–44. The lowest metabolites number was 26 in PLKU-38, while, IC-280031 and IC-139356 lines showed the presence of highest number of metabolites (44). Lines IC-280031 and IC-139356 were found most nutritive amongst selected panel of horsegram germplasm by containing maximum number of quantifiable metabolites during 1H NMR spectroscopy study. Further, the NMR based data of 96 germplasms was subjected for statistical analysis (PCA, Matrix plot, stacked charts) that revealed the similarities and variations among the whole germplasm. The methionine, sucrose, maltose, riboflavin and myricetin were observed as differential chemo-markers that help to differentiate the horsegram lines of selected panel. This information will further aid in the selection of nutritionally efficient cultivars for proteomics and genomics studies and these lines can be used as nutraceutical food for the prevention of various health ailments including kidney stone.
Madden-Julian oscillation influences United States springtime tornado and hail frequency
Authors: Miller Douglas E. , Gensini Vittorio A. , Barrett Bradford S. 
Published: May 2022

AbstractThe Madden–Julian Oscillation (MJO) is the dominant mode of intraseasonal variability in the tropics and has a documented influence on extratropical extreme weather through modulation of synoptic atmospheric conditions. MJO phase has been correlated with anomalous tornado and severe hail frequency in the United States (US). However, the robustness of this relationship is unsettled, and the variability of physical pathways to modulation is poorly understood, despite the socioeconomic impacts that tornadoes and hail evoke. We approached this problem using pentad MJO indices and practically perfect severe weather hindcasts. MJO lifecycles were cataloged and clustered to document variability and potential pathways to enhanced subseasonal tornado and hail predictability. Statistically significant increases in US tornado and hail probabilities were documented 3–4 weeks following the period of the strongest upper-level divergence for the 53 active MJO events that propagated past the Maritime continent, contrasting with the 47 MJO events that experienced the barrier effect, during boreal spring 1979–2019. The 53 MJO events that propagated past the Maritime continent revealed three prevailing MJO evolutions—each containing unique pathways and modulation of US tornado and hail frequency—advancing our knowledge and capability to anticipate these hazards at extended lead times.
Plants grown in Apollo lunar regolith present stress-associated transcriptomes that inform prospects for lunar exploration
Authors: Paul Anna-Lisa , Elardo Stephen M. , Ferl Robert 
Published: May 2022

AbstractThe extent to which plants can enhance human life support on other worlds depends on the ability of plants to thrive in extraterrestrial environments using in-situ resources. Using samples from Apollo 11, 12, and 17, we show that the terrestrial plant Arabidopsis thaliana germinates and grows in diverse lunar regoliths. However, our results show that growth is challenging; the lunar regolith plants were slow to develop and many showed severe stress morphologies. Moreover, all plants grown in lunar soils differentially expressed genes indicating ionic stresses, similar to plant reactions to salt, metal and reactive oxygen species. Therefore, although in situ lunar regoliths can be useful for plant production in lunar habitats, they are not benign substrates. The interaction between plants and lunar regolith will need to be further elucidated, and likely mitigated, to best enable efficient use of lunar regolith for life support within lunar stations.
Site-specific photolabile roadblocks for the study of transcription elongation in biologically complex systems
Authors: Nadon Jean-François , Epshtein Vitaly , Cameron Etienne , Samatov Mikhail R. , Vasenko Andrey S. , Nudler Evgeny , Lafontaine Daniel A. 
Published: May 2022

AbstractTranscriptional pausing is crucial for the timely expression of genetic information. Biochemical methods quantify the half-life of paused RNA polymerase (RNAP) by monitoring restarting complexes across time. However, this approach may produce apparent half-lives that are longer than true pause escape rates in biological contexts where multiple consecutive pause sites are present. We show here that the 6-nitropiperonyloxymethyl (NPOM) photolabile group provides an approach to monitor transcriptional pausing in biological systems containing multiple pause sites. We validate our approach using the well-studied his pause and show that an upstream RNA sequence modulates the pause half-life. NPOM was also used to study a transcriptional region within the Escherichia coli thiC riboswitch containing multiple consecutive pause sites. We find that an RNA hairpin structure located upstream to the region affects the half-life of the 5′ most proximal pause site—but not of the 3′ pause site—in contrast to results obtained using conventional approaches not preventing asynchronous transcription. Our results show that NPOM is a powerful tool to study transcription elongation dynamics within biologically complex systems.
Integrated omics approach to unveil antifungal bacterial polyynes as acetyl-CoA acetyltransferase inhibitors
Authors: Lin Ching-Chih , Hoo Sin Yong , Ma Li-Ting , Lin Chih , Huang Kai-Fa , Ho Ying-Ning , Sun Chi-Hui , Lee Han-Jung , Chen Pi-Yu , Shu Lin-Jie , Wang Bo-Wei , Hsu Wei-Chen , Ko Tzu-Ping , Yang Yu-Liang 
Published: May 2022

AbstractBacterial polyynes are highly active natural products with a broad spectrum of antimicrobial activities. However, their detailed mechanism of action remains unclear. By integrating comparative genomics, transcriptomics, functional genetics, and metabolomics analysis, we identified a unique polyyne resistance gene, masL (encoding acetyl-CoA acetyltransferase), in the biosynthesis gene cluster of antifungal polyynes (massilin A 1, massilin B 2, collimonin C 3, and collimonin D 4) of Massilia sp. YMA4. Crystallographic analysis indicated that bacterial polyynes serve as covalent inhibitors of acetyl-CoA acetyltransferase. Moreover, we confirmed that the bacterial polyynes disrupted cell membrane integrity and inhibited the cell viability of Candida albicans by targeting ERG10, the homolog of MasL. Thus, this study demonstrated that acetyl-CoA acetyltransferase is a potential target for developing antifungal agents.