bluuebunny/crossref_metadata_2025_split huggingface dataset:
GLP1R inhibits the progression of endometrial carcinoma through activation of cAMP/PKA pathway
Authors: Li Wu , Gu Yanpin , Liu Songjun , Ruan Fan , Lv Wen 
Published: August 2022

AbstractBackgroundThis study strived to explore the role and mechanism of glucagon‐like peptide‐1 receptor (GLP1R) in endometrial carcinoma (EC).MethodsIn detail, after transfection of GLP1R overexpression vector and small interfering RNA targeting PKA, the mRNA expressions of GLP1R and PKA in EC cells (Ishikawa and RL95‐2) were quantified by quantitative reverse transcription polymerase chain reaction (qRT‐PCR). The cell biological behaviors, including proliferation, migration, invasion, and apoptosis, were detected using 5‐ethynyl‐2′‐deoxyuridine (EdU), wound healing, transwell, and flow cytometry assays, respectively. The cyclic adenosine monophosphate (cAMP) content and related protein expressions (GLP1R, p‐PKA, and PKA) were determined by enzyme‐linked immunosorbent assay (ELISA) and western blot. The effects of GLP1R and PKA on tumorigenesis were evaluated by measuring the tumor volume and weight of mice bearing EC.ResultAccording to the results, GLP1R expression was downregulated in EC tissues and cells, and there was a positive correlation between GLP1R and PKA expressions. Upregulation of GLP1R promoted apoptosis and activated the cAMP/PKA signaling pathway in EC cells, while hindering the EC cell proliferation, invasion, migration, and the growth of tumor in mice. However, these effects were blunted by downregulation of PKA, which also accelerated the progression of EC in vitro and in vivo via inhibiting the activation of cAMP/PKA signaling pathway.ConclusionCollectively, upregulation of GLP1R impeded EC progression via inducing the activation of cAMP/PKA signaling pathway, which may be a potential treatment for EC.
A peptide translated from circPPP1R12A promotes the malignancy of non‐small cell lung cancer cells through AKT signaling pathway
Authors: Zhao Weijun , Xue Yibo , Zhang Yandan , Zhu Yonggang , Chen Zixuan , Zhao Xiaodong 
Published: August 2022

AbstractBackgroundRecent literature have indicated that the malignancy of cancer cells is modulated by hsa_circ_0000423 (named circPPP1R12A) through the way of translating protein. Herein, we investigated the role and latent mechanisms of circPPP1R12A in Non‐Small Cell Lung Cancer (NSCLC).MethodsCircPPP1R12A expression was measured by qRT‐PCR. The malignancy of NSCLC was determined by CCK‐8, TUNEL assay, Wound healing, Transwell and Western blotting assays. The underlying mechanisms of circPPP1R12A were confirmed by Western blotting and qRT‐PCR assays.ResultsCircPPP1R12A expression in NSCLC tissues was higher than that of neighboring normal tissues. CircPPP1R12A showed an upregulated expression in NSCLC cells. Upregulation of circPPP1R12A could promote the cell viability of NSCLC cells and reduce the apoptosis of NSCLC cells, while it could not promote cell invasion and migration. The reduction of cell viability and apoptosis was discovered in NSCLC cells with the silencing of circPPP1R12A, but circPPP1R12A knockdown does not inhibit cell invasion and migration. There was something interesting that circPPP1R12A encoding protein circPPP1R12A‐73aa was found in NSCLC cells. Mutations in circPPP1R12a‐73AA might disrupt the function of circPPP1ra‐73AA in A549 and H1299 cells. Next, we found that circPPP1R12A caused the increased growth of NSCLC cells by activating AKT signaling pathway.ConclusionIn summary, our study proved that NSCLC cell proliferation was promoted by circPPP1R12A‐73aa translated from circPPP1R12A through the AKT pathway, which could throw some light on the understanding of the mechanism of NSCLC.
Flow cytometry assessment of reactive T‐cells distinguishes classic Hodgkin lymphoma from benign lymphadenopathy in children
Authors: Gao Hui , Cai Fengqing , Liu Lei , Shen Hongqiang 
Published: August 2022

AbstractBackgroundDetection of classic Hodgkin lymphoma (cHL) neoplastic cells using flow cytometric immunophenotyping (FCI) remains limited. We hypothesized that characterization of the reactive infiltrates could assist in diagnosing cHL in children.MethodsFCI using four‐color staining approaches was performed on 156 lymph node specimens with the following histopathologic diagnoses: cHL (25 cases), reactive lymphoid hyperplasia (RLH, 44 cases), and non‐Hodgkin lymphoma (87 cases).ResultsThe overall concordance of FCI data with the histopathologic results of these cases was 81.4%. A reactive expansion of T‐cells with increased expression of CD45RO was present in the reactive infiltrate of cHL (CD45RO/CD3, 67.5%) and Epstein–Barr virus (EBV) infected RLH (62.7%) but not in EBV‐negative RLH (28.0%). The mean fluorescence intensity (MFI) of CD7 was higher for cHL and differed significantly from EBV‐positive RLH (138.5 vs. 63.8). A proposed diagnostic algorithm markedly elevated the overall concordance rate from 81.4% to 97.4%.ConclusionsImmunophenotyping the reactive infiltrate of lymphoid tissue using flow cytometry is a reliable supplement to histopathology for the rapid diagnosis of pediatric cHL.
Leadership behaviors, attitudes and characteristics to support a culture of safety
Authors: Montminy Susan L. 
Published: August 2022

AbstractIt has been estimated that medical errors are the third highest‐ranking cause of death in the United States. A patient safety culture has been touted for many years as best practice to reduce medical error. While there is a general agreement of its importance, it has remained elusive for many. This study sought to learn how strengthening leadership skills within a health care organization could catalyze patient safety culture improvement. The research asked the following to gain an appreciation for that question: How does a leader ensure consistency in policies, practices, and protocols to create a patient safety culture? What attitudes, beliefs, and collective efficacy are needed to produce a patient safety culture? What leadership characteristics are needed to create a patient safety culture? The research participants were the employees who make up the patient safety department in a large academic health care system. Information was gathered to capture their view of leadership's role in patient safety culture and to gain knowledge relative to their individual experiences following a medical error.
MicroRNA and metabolomics signatures for adrenomyeloneuropathy disease severity
Authors: Turk Bela Rui , Poisson Laila Marie , Nemeth Christina Linnea , Goodman Jordan , Moser Ann B. , Jones Richard Owen , Fatemi Ali , Singh Jaspreet 
Published: August 2022

AbstractAdrenomyeloneuropathy (AMN), the slow progressive phenotype of adrenoleukodystrophy (ALD), has no clinical plasma biomarker for disease progression. This feasibility study aimed to determine whether metabolomics and micro‐RNA in blood plasma provide a potential source of biomarkers for AMN disease severity. Metabolomics and RNA‐seq were performed on AMN and healthy human blood plasma. Biomarker discovery and pathway analyses were performed using clustering, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and regression against patient's clinical Expanded Disability Status Score (EDSS). Fourteen AMN and six healthy control samples were analyzed. AMN showed strong disease‐severity‐specific metabolic and miRNA clustering signatures. Strong, significant clinical correlations were shown for 7‐alpha‐hydroxy‐3‐oxo‐4‐cholestenoate (7‐HOCA) (r2 = 0.83, p < 0.00001), dehydroepiandrosterone sulfate (DHEA‐S; r2 = 0.82, p < 0.00001), hypoxanthine (r2 = 0.82, p < 0.00001), as well as miRNA‐432‐5p (r2 = 0.68, p < 0.00001). KEGG pathway comparison of mild versus severe disease identified affected downstream systems: GAREM, IGF‐1, CALCRL, SMAD2&3, glutathione peroxidase, LDH, and NOS. This feasibility study demonstrates that miRNA and metabolomics are a source of potential plasma biomarkers for disease severity in AMN, providing both a disease signature and individual markers with strong clinical correlations. Network analyses of affected systems implicate differentially altered vascular, inflammatory, and oxidative stress pathways, suggesting disease‐severity‐specific mechanisms as a function of disease severity.
Abusive supervision and knowledge hiding: The moderated mediation role of overall justice and gender
Authors: Koay Kian Yeik , Lim Pang Kiam 
Published: August 2022

AbstractThe purpose of this study is to examine the influence of abusive supervision on knowledge hiding via overall justice based on social exchange theory. In addition, using a moderated mediation framework, gender is proposed and tested as a moderator of the relationship between abusive supervision and knowledge hiding through overall justice, grounded in social role theory. Two hundred and forty‐three participants are recruited from public listed companies in Malaysia. Partial least squares structural equation modeling (PLS‐SEM) is used as the main analytic tool. The results reveal that abusive supervision has a significant negative influence on overall justice. In addition, both the influence of overall justice on knowledge hiding and the mediating effect of overall justice between abusive supervision and knowledge hiding is not significant. However, the influence of abusive supervision on knowledge hiding through overall justice is significant for males but not for females. The study contributes to the knowledge‐hiding literature by providing new insights into how males and females cope with abusive supervision to restore equity.
Derivation and validation of a prediction model for patients with lung nodules malignancy regardless of mediastinal/hilar lymphadenopathy
Authors: Liu Fenghui , Dai Liping , Wang Yulin , Liu Man , Wang Meng , Zhou Zhigang , Qi Yu , Chen Ruiying , OuYang Songyun , Fan Qingxia 
Published: August 2022

AbstractBackgroundClinical prediction models to classify lung nodules often exclude patients with mediastinal/hilar lymphadenopathy, although the presence of mediastinal/hilar lymphadenopathy does not always indicate malignancy. Herein, we developed and validated a multimodal prediction model for lung nodules in which patients with mediastinal/hilar lymphadenopathy were included.MethodsA single‐center retrospective study was conducted. We developed and validated a logistic regression model including patients with mediastinal/hilar lymphadenopathy. Discrimination of the model was assessed by area under the operating curve. Goodness of fit test was performed via the Hosmer–Lemeshow test, and a nomogram of the logistic regression model was drawn.ResultsThere were 311 cases included in the final analysis. A logistic regression model was developed and validated. There were nine independent variables included in the model. The aera under the curve (AUC) of the validation set was 0.91 (95% confidence interval [CI]: 0.85–0.98). In the validation set with mediastinal/hilar lymphadenopathy, the AUC was 0.95 (95% CI: 0.90–0.99). The goodness‐of‐fit test was 0.22.ConclusionsWe developed and validated a multimodal risk prediction model for lung nodules with excellent discrimination and calibration, regardless of mediastinal/hilar lymphadenopathy. This broadens the application of lung nodule prediction models. Furthermore, mediastinal/hilar lymphadenopathy added value for predicting lung nodule malignancy in clinical practice.
Hemi‐marsh concept prevails? Kaminski and Prince (1981) revisited
Authors: Masto Nicholas M. , Kaminski Richard M. , Prince Harold H. 
Published: August 2022

AbstractThe hemi‐marsh concept has prevailed in avian habitat selection and wetland ecology and management since the 1970s. Hemi‐marsh is a stage of wetland succession when approximately equal proportions of emergent vegetation and open water occur. In 1981, researchers reported on a field experiment in Delta Marsh, Manitoba, Canada, where they artificially created interspersion levels of emergent vegetation and open water (30%:70%, 50:50 [hemi‐marsh], and 70:30) by mowing or rototilling to test for hypothesized differences in indicated breeding pair indices (IBPs/ha; pairs + lone males) of dabbling ducks and species diversity among treatment combinations. Greatest dabbler densities and species diversity were in 50:50 hemi‐marsh plots. This research has been widely cited; however, the experiment was pseudo‐replicated because of non‐independence of IBP counts within and among weeks and years of surveys. We reanalyzed data from the study to support or refute original results. More robust and generalized analyses revealed vegetation:water interspersion characteristics influenced IBP densities of blue‐winged teal (Spatula discors), mallard (Anas platyrhynchos), gadwall (Mareca strepera), northern shoveler (S. clypeata), and species diversity. We estimated greatest IBP densities and species diversity in 50:50 hemi‐marsh plots, agreeing with the previous study. Including nested random effects to account for temporal pseudo‐replication improved model fits by 2–30%. Despite accounting for temporal variation in dabbler density and species diversity, 61–93% of variation remained unexplained, indicating unresolved complexities of breeding dabbler habitat use. Nonetheless, our reanalysis generalized and strengthened the original finding that dabbling duck densities and species diversity were greatest in hemi‐marsh plots. Managers may strive to reproduce hemi‐marsh conditions for dabbling ducks and evaluate other waterbird responses. Our reanalysis also emphasized the need for researchers to account for or prevent temporal and spatial pseudo‐replication, examine model fit, and provide reproducible documentation of data and code, especially now that analytical tools and databases are freely available.
Synthesis by Sol‐Gel Route, Chemical, and Biological Characterization of Hybrid Material Composed by Fe(II)C and poly(ε‐caprolactone)
Authors: Catauro Michelina , Blanco Ignazio , Fiorentino Marika , D'Angelo Antonio , Dell'Anna Maria Michela , Mastrorilli Piero 
Published: August 2022

AbstractSol–gel route represents a valuable technique to obtain functional materials, in which organic and inorganic components are closely connected. Imbalance of the iron level in the body causes several diseases. In particular, the low level of iron, during pregnancy, is responsible of the iron deficiency anemia, and even of neurodegenerative diseases. The aim of this work is the synthesis of iron (II) therapeutic systems by sol–gel method. Different weight percentages of poly(ε‐caprolactone) (PCL 7wt%) and ferrous citrate (Fe(II)C 6wt%) for drug delivery applications are embedded in a SiO2 matrix. Fourier transform infrared (FTIR) spectroscopy is used to study the interactions among different components in the hybrid materials. SiO2/Fe(II)C/PCL materials have been proposed as valuable antibacterial agents against Escherichia coli and Enterococcus faecalis.
Tribological Behavior of UHMWPE (Disc) against Ti6Al4V (Pin) under Different Lubrication Conditions
Authors: Visco Annamaria , Grasso Antonio , Scolaro Cristina , Belhamdi Hossem , Sili Andrea 
Published: August 2022

AbstractThe proposed research work is addressed to the study of the wear behavior of Ultra High Molecular Weight Polyethylene (UHMWPE) under the contact with a rounded pin in Ti6Al4V alloy, which is largely used for production of the last generation of prosthetic implants by electron beam manufacturing (EBM). The wear tests of UHMWPE in contact with a Ti6Al4V pin are carried out dry or in the presence of lubricants fluids that are characterized by tribological tests, wet‐ability measurements, and morphological observations of the contact surfaces. UHMWPE wear resistance improves in conditions of lubrication in the order: dry < distilled water < synthetic synovial < bovine serum, according to wettability results. As suggested by the track profiles, the contact action in dry can be mainly due to shear stress while that in lubricant mainly to compressive stress.
Thermal Influence on Physico‐Chemical Properties of Geopolymers Based on Metakaolin and Red Tomato Waste
Authors: Catauro Michelina , D'Angelo Antonio , Piccolella Simona , Leonelli Cristina , Dal Poggetto Giovanni 
Published: August 2022

AbstractThe high amount of organic and inorganic wastes has increased the attention to new strategies aiming to reduce the waste disposals. Organic wastes, such as tomato wastes (TWs), are a good source from which the red color can be obtained. Among the different technologies, the geopolymers had been proposed as a powerful technology able to incorporate various kinds of wastes. In this paper, pure metakaolin and a mixture obtained by adding 10% of red TW‐derived (peels) are consolidated by alkali activation at room temperature, 40 and 60°C without the pigment extraction. Fourier‐transform infrared (FTIR) spectra confirmed the geopolymerization occurrences. Moreover, the obtained materials are analyzed for their conductivity and pH after the sample extractions at different times. The integrity tests assessed the resistance of the synthesized geopolymers and the presence of red tomato‐wastes led to a release of yellow organic hydro‐soluble compounds. Finally, the weight loss confirmed the integrity test. Indeed, there are no differences at 16 and 30 d.
Nanocomposite Based on a Natural Anionic Polysaccharide for Anticancer Drug Immobilization
Authors: Spiridonov Vasily , Shibaeva Anna , Kusaia Victoriia , Zoirova Zukhra , Antonova Yulia , Yaroslavov Alexander 
Published: August 2022

AbstractSynthesis of polymer nanocomposite as nanocontainers for doxorubicin (DOX) is carried out at a molar ratio of monomer units of the anionic polysaccharide (sodium hyaluronate) and Ca2+‐ions as a cross‐linking agent equaled to 10/1. Highly water‐soluble white fibrous products can be prepared. The hydrodynamic diameter of nanocompsite (Ca2+/Na‐Hyaluronate) particles is equal to 150 nm. At the same time, diameter of the original sodium hyaluronate (Na‐Hyaluronate) macromolecules is about 300 nm. The interaction between synthesized nanocomposite particles, Ca2+/Na‐Hyaluronate, and DOX accompanied by a new complex formation can be confirmed by means of fluorimetry. Obtained results indicate that the nanocomposite is capable as an immobilizing doxorubicin nanocontainer.
Inclusion Complexes Between β‐cyclodextrin and the Anticancer Drug 5‐Fluorouracil for its Solubilization: a Molecular Dynamics Study at Different Stoichiometries
Authors: Raffaini Giuseppina , Elli Stefano , Ganazzoli Fabio , Catauro Michelina 
Published: August 2022

AbstractCyclodextrins (CDs) are natural macrocyclic oligosaccharides able to solubilize hydrophobic drugs in water improving their bioavailability, thanks to favorable noncovalent interactions particularly in their hydrophobic cavity. Using a simulation protocol proposed in previous work, in this theoretical study based on Molecular Mechanics (MM) and Molecular Dynamics (MD) methods, inclusion complexes between β‐cyclodextrin and 5‐Fluorouracil (5‐FU) are investigated. 5‐FU is an anticancer drug hardly soluble in water used for more than 50 years. This drug is mainly administered by intravenous injection for the treatment of inner cancers or as a cream in the case of skin‐related cancers. For drug delivery applications, it is important to characterize stable inclusion complexes at different drug concentrations in order to enhance the anticancer activity by maintaining lower dose, thus reducing cytotoxic effects on the normal cells, causing several negative impacts in the course of treatment. In this work, the structure and stability of the host–guest complexes β‐CD:5‐FU in a 1:1 and 1:2 stoichiometries are investigated because this drug is relatively small compared to the size of the hydrophobic β‐CD cavity. The 5‐FU complexes with CDs allow its solubilization, thanks to favorable van der Waals interactions. Interestingly, these intermolecular interactions in inclusion complexes may also affect the release profile. MD simulations are a powerful tool to evaluate interactions between hydrophobic anticancer drugs and hydrophilic carriers with an inner hydrophobic cavity such as CDs and oligosaccharides in general.
Redesign of a 3D Low Cost Filament Printer, Adapting it to a Pellet Extruder for New Material Assays
Authors: Mosquera Alberto , Andrade Pamela , Cobos Christian , Cárdenas Eugenio , Ferrándiz Santiago 
Published: August 2022

AbstractThe present work arises from the need identified on the additive manufacturing field due to the lack of 3D low cost pellet printers and the little diversity of materials available for 3D FFF printing. Considering this problem, a 3D pellet printer design is proposed starting from a 3D FFF printer. To carry out this design work, at first it is selected a commercial pellet extruder which meets all the conditions to provide a proper printing quality. At second, a new mechanical propose is analyzed and tested by simulation programs considering the changes made. In the course of the mechanical analysis, the structure is reviewed, and also a temperature study is accomplished. Based on the results, it is possible to verify the correct performance of the machine. From the different simulations, the optimal conditions in terms of dimensioning, structure, internal temperatures, and loads are obtained. This enables to determine an optimal and functional design which adapts into a commercial pellet extruder keeping low costs and simple implementation.
The Effect of the Natural and Synthetic Porphyrin Complexes on the Structure and Properties of the Semi‐Crystalline Polymers
Authors: Tyubaeva Polina , Zykova Anna , Olkhov Anatoly , Lobanov Anton 
Published: August 2022

AbstractRecently, nanocomposite fibrous materials with favorable functional properties are widely used in various fields, for example: conductive fibers, fiber‐optic sensors, materials for photonics, gene therapy, and biomedicine. The development of modern nanocomposite fibrous materials with valuable functional properties based on biodegradable semicrystalline polymers is an actual scientific problem. Moreover, the use of natural and biodegradable polymers as objects allows to approach the solution of an environmental problem, expanding the list of known ecofriendly materials for special purposes, expanding the areas of their practical application, including solving biomedical problems. Thus, the aim of the work is the development of a methodology for the directed design of biodegradable fibrous nanocomposites with specified properties. In the article, the methods of directed modification of semicrystalline polymers of natural origin (polyhydroxybutyrate) by modifying additives based on natural porphyrin complexes (hemin) are considered. Electrospinning is one of the most productive methods to obtain a highly developed well oriented structure with specified characteristics. The influence of additives on the polymeric fibrous structure and, as a result, on the properties of the electrospun material are investigated in the work.
Photo‐Degradation of a Biopolyester Blend under UV‐C Rays
Authors: Visco Annamaria , Scolaro Cristina , Bellhamdi Hossem , Grasso Antonio 
Published: August 2022

AbstractBiopolyesters, pure and mixed with a compatibilizer, are exposed to UV‐C radiation in the interval 4–32 h. UV‐C rays act as germicides and antibacterial on biomedical materials. The mechanical and physical characterization tests have shown how all the materials, pure and mixed, undergo UV‐C photodegradation even if in a different way. The change in the structural order of the polymer, the embrittlement, and the change in the surface wettability of the water/blood is lower in the blend than in pure polymers. This is thanks to the cross‐linked structure of the mixture, which is more thermally stable, mechanically resistant, and more hydrophobic than the individual components.