bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biogeographical distribution of Hartaetosiga strains (Choanoflagellatea, Craspedida, Salpingoecidae) including morphological and transcriptomic data from a transect across the Atlantic Ocean
Authors: Schiwitza Sabine , Spruck Christiane , Nitsche Frank 
Published: June 2022

AbstractThe genus Hartaetosiga Carr, Richter and Nitsche, 2017 comprised up to now only three species, H. gracilis (Kent) Carr, Richter, Nitsche, 2017, H. balthica (Wylezich and Karpov) Carr, Richter and Nitsche, 2017 and H. minima (Wylezich and Karpov) Carr, Richter and Nitsche, 2017. Based on distinct molecular data these species were relocated from the strictly freshwater genus Codosiga (Ehrenberg) Bütschli, 1878 to a new genus comprising brackish and marine species. During the cruise MSM82/2 across the Atlantic Ocean in 2019, surface water samples were taken from 15 stations along a transect ranging from 35°S to 23°N. We were able to isolate and cultivate 14 strains of the genus Hartaetosiga. Morphometric data showed no distinct morphological traits allowing for a species delineation, indicating a cryptic species complex within the genus. Based on cultivation, morphological data, and molecular analyses, we recorded H. gracilis for the first time from off‐shelf waters of the Atlantic Ocean and could describe a new species, H. australis n. sp. This new species was recorded from sampling stations in the Southern Hemisphere only, which may indicate a potential biogeographic restriction likely caused by the Equatorial Counter Current (ECC), dividing the northern and southern surface waters.
Evolutionary convergence in body shape obscures taxonomic diversity in species of the African Labeo forskalii group: Case study of L. parvus Boulenger 1902 and L. ogunensis Boulenger 1910
Authors: Liyandja Tobit L.D. , Armbruster Jonathan W. , Poopola Michael O. , Stiassny Melanie L. J. 
Published: June 2022

AbstractLabeo is the third most diverse genus of African cyprinids and is widely distributed across the continent. Labeo parvus, a small species originally described from the Congo basin, has been considered the only species of the L. forskalii group distributed across five African ichthyofaunal provinces (Nilo‐Sudan, Congo, Cuanza, and Upper and Lower Guinea). However, morphological similarity between L. parvus and numerous congeners remains a central cause of taxonomic confusion within the genus. Here we employed a phylogenetic comparative approach to assess phenotypic convergence among species of the L. forskalii group, investigate the taxonomic status of L. parvus sensu lato (sl) in west Africa, and reevaluate the composition and distribution of L. parvus sensu stricto (ss). Our phylogenetic analysis provides no support for a sister relationship between L. parvus ss and any of the west African Labeo parvus‐like species. Geometric morphometric and molecular phylogenetic data indicate that L. parvus ss is a Congo basin endemic, and seemingly ecologically equivalent species found in west Africa are L. ogunensis, L. obscurus and other undescribed or previously synonymized species. We discuss our findings in terms of convergent evolution using phylomorphospace and tests for phylogenetic signal.
Development and characterization of a skin cell line from Chinese perch (Siniperca chuatsi) and its application in aquatic animal viruses
Authors: Meng Xian‐Yu , Wang Zi‐Hao , Yu Xue‐Dong , Zhang Qi‐Ya , Ke Fei 
Published: June 2022

AbstractChinese perch (Siniperca chuatsi), an important fish for the aquaculture industry of China, is often affected by viral diseases. A stable and sensitive cell line can play an important role in virus identification and isolation, functional gene identification, virus pathogenic mechanism and antiviral immunity study. In the present study, a new cell line (S. chuatsi skin cell, SCSC) derived from the skin of S. chuatsi was established. The SCSC mainly consisted of fibroblastic‐like cells, which grew well in M199 medium supplemented with 10% foetal bovine serum at 25°C. Chromosome analysis revealed that the SCSC (44%) has a diploid chromosome number of 2n = 48. The SCSC can be transfected and expressed exogenous gene efficiently. It also showed high sensitivity to several aquatic animal viruses from different families including Rhabdoviridae, Iridoviridae and Reoviridae. In addition, RT‐PCR showed that S. chuatsi rhabdovirus (SCRV) started genome replication as early as 3 h post infection in the cells, which also induced the up‐regulation of a variety of immune‐related genes including these related to interleukin family, pattern recognition receptors, JAK–STAT pathway and interferon regulatory factors. In summary, current study provided a new tool in research of fish viruses and its interaction with host.
Development of a nutrition screening tool to identify need for dietetic intervention in female infertility
Authors: Deepak Kumar Komal , Huntriss Rosemary , Green Eulalee , Bora Shabana , Pettitt Claire 
Published: June 2022

AbstractBackgroundOne in seven couples are impacted by infertility in the UK, and female infertility is often associated with several health conditions impacted by nutrition. Despite many studies aimed at identifying the critical role of nutrition in infertility, there is currently no screening tool that identifies nutritional risk factors for infertility.AimTo propose a self‐administered screening tool to identify women who would benefit from nutritional intervention to promote fertility.MethodsA narrative review was carried out to identify and summarise modifiable nutritional risk factors that can influence female fertility, including comorbidities that can influence nutrition intake, absorption, and metabolism.Key FindingsA nutrition screening tool outlining modifiable nutrition risk factors potentially improving female fertility has been proposed, comprising of BMI, medical history and quality of diet and lifestyle which would aid in designing evidence based dietetic services for female infertility.
Outcomes of paediatric patients with chronic liver disease in early adulthood: A heterogeneous, but representative, regional cohort study
Authors: Lau Nicole SM , Henderson Paul 
Published: June 2022

AimAdvances in paediatric hepatology have led to the increasing survival of patients with paediatric‐onset chronic hepatobiliary disease into adulthood. Data are lacking with regard to the outcomes of this heterogeneous group of patients and current transition models may be insufficient. This retrospective regional cohort study examined the outcomes of these patients cared for in a paediatric gastroenterology centre following transfer to adult services.MethodsA prospective database of paediatric patients with liver disease identified those already transferred to adult services. Following exclusions, medical notes were examined and health parameters recorded including initial diagnoses, transplant status, fertility and mortality. Descriptive statistics were used to calculate follow‐up data and transplant‐free survival (TFS).ResultsOverall, 63 patients (52% male) entered the final analyses with a median follow‐up of 27.5 years. The most common diagnosis was biliary atresia (19%); 27 different diagnoses were apparent within the cohort highlighting the heterogeneity within a single centre. Transplant prevalence at adult transfer was 41%; 14% of patients were lost to follow‐up including 10% of transplant patients. TFS for biliary atresia was 17% after 37.4 years follow‐up and was 54% for the total cohort. There were seven documented pregnancies and the prevalence of any psychological or psychiatric input was 44%. Transplant complications occurred in 38% of patients; there were two cancer diagnoses and two deaths following transfer.ConclusionsAlthough overall mortality was low, the health‐care burden of patients with paediatric‐onset chronic liver disease is high. This group is also very heterogeneous, making structured transition to adult services difficult.
Optimising sounds for the driving of sleep oscillations by closed‐loop auditory stimulation
Authors: Debellemanière Eden , Pinaud Clémence , Schneider Jules , Arnal Pierrick J. , Casson Alexander J. , Chennaoui Mounir , Galtier Mathieu , Navarrete Miguel , Lewis Penelope A. 
Published: June 2022

SummaryRecent studies have shown that slow oscillations (SOs) can be driven by rhythmic auditory stimulation, which deepens slow‐wave sleep (SWS) and improves memory and the immune‐supportive hormonal milieu related to this sleep stage. While different attempts have been made to optimise the driving of the SOs by changing the number of click stimulations, no study has yet investigated the impact of applying more than five clicks in a row. Likewise, the importance of the type of sounds in eliciting brain responses is presently unclear. In a study of 12 healthy young participants (10 females; aged 18–26 years), we applied an established closed‐loop stimulation method, which delivered sequences of 10 pink noises, 10 pure sounds (B note of 247 Hz), 10 pronounced “a” vowels, 10 sham, 10 variable sounds, and 10 “oddball” sounds on the up phase of the endogenous SOs. By analysing area under the curve, amplitude, and event related potentials, we explored whether the nature of the sound had a differential effect on driving SOs. We showed that every stimulus in a 10‐click sequence, induces a SO response. Interestingly, all three types of sounds that we tested triggered SOs. However, pink noise elicited a more pronounced response compared to the other sounds, which was explained by a broader topographical recruitment of brain areas. Our data further suggest that varying the sounds may partially counteract habituation.
Control of ticks on horses using abamectin‐impregnated ear tags. A pharmacokinetic and pharmacodynamic study
Authors: Werners Arno , Karasek Inga , Butler Catherine , Yeatts James , Enomoto Hiroko , Baynes Ronald 
Published: June 2022

AbstractSeveral different tick species are known to infest horses. Aside from causing serious health and welfare issues, including anaemia, ill thrift, and immunosuppression, ticks can transmit a variety of important, sometimes zoonotic, pathogens. The successful prevention and treatment of tick infestations have been described, but the information is scarce and, in many instances, anecdotal. Here we describe a practical and affordable prevention of tick infestation by using abamectin‐impregnated cattle ear tags affixed to a safety collar. We have assessed the radial distribution of abamectin by analyzing hair samples, as well as its efficacy against tick infestations. The study results show that abamectin distributes across horse skin from the site of application and its associated effectiveness in reducing the tick burden.
Competency frameworks, nursing perspectives, and interdisciplinary collaborations for good patient care: Delineating boundaries
Authors: Zumstein‐Shaha Maya , Grace Pamela J. 
Published: June 2022

AbstractTo enhance patient care in the inevitable conditions of complexity that exist in contemporary healthcare, collaboration among healthcare professions is critical. While each profession necessarily has its own primary focus and perspective on the nature of human healthcare needs, these alone are insufficient for meeting the complex needs of patients (and potential patients). Persons are inevitably contextual entities, inseparable from their environments, and are subject to institutional and social barriers that can detract from good care or from accessing healthcare. These are some of the reasons behind current movements to develop competency frameworks that can enhance cross‐disciplinary communication and collaboration. No single profession can claim the big picture. Effective teamwork is essential and requires members of diverse professions to understand the nature of each other's knowledge, skills, roles, perspectives, and perceived responsibilities so that they are optimally utilized on behalf of patients and their families. Interdisciplinary approaches to care permit different aspects of a person's needs to be addressed seamlessly and facilitate the removal of obstacles by engaging the range of resources exemplified by the different professions. Additionally, collaborative efforts are needed to influence policy changes on behalf of individual and social good and to address root causes of poor health especially as these impact society's most vulnerable. Here, we explore both the benefits and the risks of an uncritical acceptance of competency frameworks as a way to enhance interdisciplinary communication. We highlight the importance of anchoring proposed competency domains in the reason for being of a given profession and exemplify one way this has been accomplished for advanced practice nursing. Additionally, we argue that having this mooring, permits integration of the various competencies that both enhances professional moral agency and facilitates interdisciplinary collaboration to further the mutual goals of the healthcare professions on behalf of quality patient care.
The supercontinent cycle and Earth's long‐term climate
Authors: Nance R. Damian 
Published: June 2022

AbstractEarth's long‐term climate has been profoundly influenced by the episodic assembly and breakup of supercontinents at intervals of ca. 500 m.y. This reflects the cycle's impact on global sea level and atmospheric CO2 (and other greenhouse gases), the levels of which have fluctuated in response to variations in input from volcanism and removal (as carbonate) by the chemical weathering of silicate minerals. Supercontinent amalgamation tends to coincide with climatic cooling due to drawdown of atmospheric CO2 through enhanced weathering of the orogens of supercontinent assembly and a thermally uplifted supercontinent. Conversely, breakup tends to coincide with increased atmospheric CO2 and global warming as the dispersing continental fragments cool and subside, and weathering decreases as sea level rises. Supercontinents may also influence global climate through their causal connection to mantle plumes and large igneous provinces (LIPs) linked to their breakup. LIPs may amplify the warming trend of breakup by releasing greenhouse gases or may cause cooling and glaciation through sulfate aerosol release and drawdown of CO2 through the chemical weathering of LIP basalts. Hence, Earth's long‐term climatic trends likely reflect the cycle's influence on sea level, as evidenced by Pangea, whereas its influence on LIP volcanism may have orchestrated between Earth's various climatic states.
Exploring ways to enhance pain management for older people with dementia in acute care settings using a Participatory Action Research approach
Authors: Harkin Deirdre , Coates Vivien , Brown Donna 
Published: June 2022

AbstractBackgroundDementia is a progressive condition that leads to reduced cognition, deteriorating communication and is a risk factor for other acute and chronic health problems. The rise in the prevalence of dementia means untreated pain is becoming increasingly common with healthcare staff being challenged to provide optimal pain management. This negatively impacts the person living with dementia and their carers. There is minimal evidence that explores the pain management experience of patients as they move through acute care settings.ObjectiveTo understand the complexities of managing the pain of older people with dementia as they progress through acute care settings, with the view of assisting staff to improve practice.MethodA Participatory Action Research approach, guided by the Promoting Action Research in Health Services framework, was used. Three Action Cycles were completed comprising of an exploratory audit and two case studies (Action Cycle One), three focus groups with a total of 14 participants (Action Cycle Two) and the development and implementation of immediate and long‐term actions (Action Cycle Three).ResultsThematic analysis identified four themes that affected pain management practices. These were not knowing the patient; balancing competing priorities; knowledge and understanding of pain and dementia and not assimilating available information.ConclusionPain management practices for patient living with dementia, across acute care settings, was influenced by shared ways of thinking and working. Not knowing the patient, fragmentation of information and having insufficient knowledge of the subtleties of dementia led participants to deliver task‐focused, target and policy‐driven care that was not person‐centred in its approach. Facilitated reflection enabled acute care teams to actively participate in identifying problems and finding solutions to enhance practice.
Preoperative Use of Aspirin in Total Knee Arthroplasty: Safe or Not?
Authors: Li Zeng , Xiang Shuai , Du Yan , Zhang Mo , Bian Yanyan , Feng Bin , Weng Xisheng 
Published: June 2022

ObjectiveTo compare the blood loss, transfusion rates and complications between the aspirin and non‐aspirin group in unilateral and bilateral total knee arthroplasties (TKAs) with a nested case–control design.MethodsThe present study retrospectively selected TKA cases from the Joint Arthroplasty Database at the Peking Union Medical College Hospital from January 2014 to December 2019 following strict inclusion and exclusion criteria, and divided them into the aspirin and non‐aspirin group based on the use of aspirin preoperatively. Bleeding was measured by blood loss, transfusion rate, drainage volume, hemoglobin (HGB) and hematocrit (HCT), while complications (cardiovascular events, venous thromboembolism events, cerebrovascular events and wound events) were compared between the groups. Student's unpaired t‐test and Mann–Whitney U‐test were used to compare the differences of continuous variables between the two groups while chi‐square test and Fisher's exact test were applied in categorical variables.ResultsA total of 560 patients with unilateral TKA and 285 patients with bilateral TKA were extracted. Among these, 280 patients used aspirin preoperatively. No other differences were found in demographic and surgical characteristics between the two groups except for the proportion of coronary artery diseases (P < 0.001). For primary outcomes, there was no significant higher blood loss and transfusion rate in the aspirin group, while the drainage of aspirin group was higher than the control group in bilateral TKAs (P = 0.043). The HGB and HCT of the aspirin group was significant lower in both unilateral and bilateral TKAs at POD5 (P < 0.05). For complications, there was a lower vascular related complication rate in aspirin group after unilateral TKAs (P = 0.040), but the wound event rate in aspirin group was higher than the control group (P = 0.049).ConclusionsPreoperative use of aspirin could prevent vascular related events during the perioperative period of TKA. However, it might also increase the risk of bleeding and wound complications.
Deep learning fusion of satellite and social information to estimate human migratory flows
Authors: Runfola Daniel , Baier Heather , Mills Laura , Naughton‐Rockwell Maeve , Stefanidis Anthony 
Published: June 2022

AbstractHuman migratory decisions are driven by a wide range of factors, including economic and environmental conditions, conflict, and evolving social dynamics. These factors are reflected in disparate data sources, including household surveys, satellite imagery, and even news and social media. Here, we present a deep learning‐based data fusion technique integrating satellite and census data to estimate migratory flows from Mexico to the United States. We leverage a three‐stage approach, in which we (1) construct a matrix‐based representation of socioeconomic information for each municipality in Mexico, (2) implement a convolutional neural network with both satellite imagery and the constructed socioeconomic matrix, and (3) use the output vectors of information to estimate migratory flows. We find that this approach outperforms alternatives by approximately 10% (r2), suggesting multi‐modal data fusion provides a valuable pathway forward for modeling migratory processes.
Risk factors for mortality in patients with dengue: A systematic review and meta‐analysis
Authors: Chagas Gabriel Cavalcante Lima , Rangel Amanda Ribeiro , Noronha Luísa Macambira , Veloso Felipe Camilo Santiago , Kassar Samir Buainain , Oliveira Michelle Jacintha Cavalcante , Meneses Gdayllon Cavalcante , da Silva Junior Geraldo Bezerra , Daher Elizabeth De Francesco 
Published: June 2022

AbstractObjectiveTo investigate risk factors for mortality in dengue.MethodsWe performed a systematic review and meta‐analysis searching MEDLINE, Embase, SciELO, LILACS Bireme, and OpenGrey databases to identify eligible observational studies of patients with dengue, of both genders, aged 14 years or older, that analysed risk factors associated with mortality and reported adjusted risk measures with their respective confidence intervals (CIs). We estimated the pooled weighted mean difference and 95% CIs with a DerSimonian and Laird random‐effects model. We assessed the methodological quality using the Newcastle‐Ottawa Scale.ResultsOf 1,170 citations reviewed, 18 papers, with a total of 25,851 patients, were included in the systematic review and 12 in the meta‐analysis. Severe hepatitis (OR 29.222, 95% CI 3.876–220.314), dengue shock syndrome (OR 23.575, 95% CI 3.664–151.702), altered mental status (OR 3.76, 95% CI 1.67–8.42), diabetes mellitus (OR 3.698, 95% CI 1.196–11.433), and higher pulse rate (OR 1.039, 95% CI 1.011–1.067) are associated with mortality in patients with dengue. All studies included were classified as having a high quality.ConclusionsProper identification and management of these risk factors should be considered to improve patient outcomes and reduce the hidden burden of this neglected tropical disease. Future well‐designed studies are needed to investigate the association of other clinical, radiological, and laboratorial findings with mortality in dengue, as well as to develop prognostic models based on the risk factors found in our study.
Spatio‐temporal transcriptome profiling and subgenome analysis in Brassica napus
Authors: Wei Lijuan , Du Hai , Li Xiaodong , Fan Yonghai , Qian Mingchao , Li Yali , Wang Huiyi , Qu Cunmin , Qian Wei , Xu Xinfu , Tang Zhanglin , Zhang Kai , Li Jiana , Lu Kun 
Published: June 2022

SUMMARYBrassica napus is an important oil crop and an allotetraploid species. However, the detailed analysis of gene function and homoeologous gene expression in all tissues at different developmental stages was not explored. In this study, we performed a global transcriptome analysis of 24 vegetative and reproductive tissues at six developmental stages (totally 111 tissues). These samples were clustered into eight groups. The gene functions of silique pericarp were similar to roots, stems and leaves. In particular, glucosinolate metabolic process was associated with root and silique pericarp. Genes involved in protein phosphorylation were often associated with stamen, anther and the early developmental stage of seeds. Transcription factor (TF) genes were more specific than structural genes. A total of 17 100 genes that were preferentially expressed in one tissue (tissue‐preferred genes, TPGs), including 889 TFs (5.2%), were identified in the 24 tissues. Some TPGs were identified as hub genes in the co‐expression network analysis, and some TPGs in different tissues were involved in different hormone pathways. About 67.0% of the homoeologs showed balanced expression, whereas biased expression of homoeologs was associated with structural divergence. In addition, the spatiotemporal expression of homoeologs was related to the presence of transposable elements (TEs) and regulatory elements (REs); more TEs and fewer REs in the promoters resulted in divergent expression in different tissues. This study provides a valuable transcriptional map for understanding the growth and development of B. napus, for identifying important genes for future crop improvement, and for exploring gene expression patterns in the B. napus.