bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence of myeloid neoplasms in Spain (2002–2013): a population-based study of the Spanish network of cancer registries
Authors: Solans Marta , Sanvisens Arantza , Ameijide Alberto , Merino Susana , Rojas Dolores , Alemán Araceli , Banqueri Emilia , Chico Matilde , Marcos Ana Isabel , de Castro Visitación , Gil Leire , de Munain Arantza López , Puigdemont Montse , Sánchez Maria-José , Perucha Josefina , Ruiz-Armengol Patricia , Chirlaque Mª Dolores , Guevara Marcela , Carulla Marià , Marcos-Gragera Rafael 
Published: January 2022

AbstractComprehensive population-based data on myeloid neoplasms (MNs) are limited, mainly because some subtypes were not recognized as hematological cancers prior to the WHO publication in 2001, and others are too rare to allow robust estimates within regional studies. Herein, we provide incidence data of the whole spectrum of MNs in Spain during 2002–2013 using harmonized data from 13 population-based cancer registries. Cases (n = 17,522) were grouped following the HAEMACARE groupings and 2013-European standardized incidence rates (ASRE), incidence trends, and estimates for 2021 were calculated. ASRE per 100,000 inhabitants was 5.14 (95% CI: 5.00–5.27) for myeloproliferative neoplasms (MPN), 4.71 (95% CI: 4.59–4.84) for myelodysplastic syndromes (MDS), 3.91 (95% CI: 3.79–4.02) for acute myeloid leukemia, 0.83 (95% CI: 0.78–0.88) for MDS/MPN, 0.35 (95% CI: 0.32–0.39) for acute leukemia of ambiguous lineage, and 0.58 (95% CI: 0.53–0.62) for not-otherwise specified (NOS) cases. This study highlights some useful points for public health authorities, such as the remarkable variability in incidence rates among Spanish provinces, the increasing incidence of MPN, MDS, and MDS/MPN during the period of study, in contrast to a drop in NOS cases, and the number of cases expected in 2021 based on these data (8446 new MNs).
High-throughput formation and image-based analysis of basal-in mammary organoids in 384-well plates
Authors: Lee Soojung , Chang Jonathan , Kang Sung-Min , Parigoris Eric , Lee Ji-Hoon , Huh Yun Suk , Takayama Shuichi 
Published: January 2022

AbstractThis manuscript describes a new method for forming basal-in MCF10A organoids using commercial 384-well ultra-low attachment (ULA) microplates and the development of associated live-cell imaging and automated analysis protocols. The use of a commercial 384-well ULA platform makes this method more broadly accessible than previously reported hanging drop systems and enables in-incubator automated imaging. Therefore, time points can be captured on a more frequent basis to improve tracking of early organoid formation and growth. However, one major challenge of live-cell imaging in multi-well plates is the rapid accumulation of large numbers of images. In this paper, an automated MATLAB script to handle the increased image load is developed. This analysis protocol utilizes morphological image processing to identify cellular structures within each image and quantify their circularity and size. Using this script, time-lapse images of aggregating and non-aggregating culture conditions are analyzed to profile early changes in size and circularity. Moreover, this high-throughput platform is applied to widely screen concentration combinations of Matrigel and epidermal growth factor (EGF) or heparin-binding EGF-like growth factor (HB-EGF) for their impact on organoid formation. These results can serve as a practical resource, guiding future research with basal-in MCF10A organoids.
Ageing transitions in a network of Rulkov neurons
Authors: Biswas Dhrubajyoti , Gupta Sayan 
Published: January 2022

AbstractThe phenomenon of ageing transitions (AT) in a Erdős–Rényi network of coupled Rulkov neurons is studied with respect to parameters modelling network connectivity, coupling strength and the fractional ratio of inactive neurons in the network. A general mean field coupling is proposed to model the neuronal interactions. A standard order parameter is defined for quantifying the network dynamics. Investigations are undertaken for both the noise free network as well as stochastic networks, where the interneuronal coupling strength is assumed to be superimposed with additive noise. The existence of both smooth and explosive AT are observed in the parameter space for both the noise free and the stochastic networks. The effects of noise on AT are investigated and are found to play a constructive role in mitigating the effects of inactive neurons and reducing the parameter regime in which explosive AT is observed.
Early endoscopic treatment of symptomatic pancreatic necrotic collections
Authors: Jagielski Mateusz , Piątkowski Jacek , Jackowski Marek 
Published: January 2022

AbstractEUS-guided transmural endoscopic drainage is commonly used in the treatment of WOPN in the late phase of ANP. The role of endoscopic intervention remains unclear in the early phase of ANP. This study aimed to prospectively evaluate early endoscopic treatment of ANCs compared with endoscopic drainage of WOPN. Overall, 71 patients with ANP who underwent transmural endoscopic drainage for necrotic collections were included. Endoscopic intervention was performed within the first four weeks of ANP in 25 (35.21%) patients with ANC (Group 1) and in 46 (64.79%) patients after four weeks since the onset of ANP with WOPN (Group 2). The overall mean age of patients was 49.9 (22–79) years and 59 of them were males. The mean time of active drainage and duration of total endoscopic treatment was 26.8 and 16.9 days (P = 0.0001) and 270.8 and 164.2 days (P = 0.0001) in Groups 1 and 2, respectively. The average total number of endoscopic interventions was 9.5 and 4.5 in Groups 1 and 2, respectively (P = 0.0001). The clinical success rate, frequency of complications of endoscopic interventions, long-term success rate, and recurrence rate were not significantly different between the groups (P > 0.05 for each). Transmural endoscopic drainage is effective method of treatment of early ANCs within the first four weeks of ANP. However, compared with endoscopic intervention in WOPN, more interventions and longer duration of drainage are required.
Spatiotemporal slip distributions associated with the 2018–2019 Bungo Channel long-term slow slip event inverted from GNSS data
Authors: Seshimo Yukinari , Yoshioka Shoichi 
Published: January 2022

AbstractLong-term slow slip events (L-SSEs) have repeatedly occurred beneath the Bungo Channel in southwestern Japan with durations of several months to a couple of years, with a recurrence interval of approximately 6 years. We estimated the spatiotemporal slip distributions of the 2018–2019 Bungo Channel L-SSE by inverting processed GNSS time series data. This event was divided into two subevents, with the first on the southwest side of the Bungo Channel from 2018.3 to 2018.7 and the second beneath the Bungo Channel from 2018.8 to 2019.4. Tectonic tremors became active on the downdip side of the L-SSE occurrence region when large slow slips took place beneath the Bungo Channel. Compared with the previous Bungo Channel L-SSEs, this spatiotemporal slip pattern and amount were similar to those of the 2002–2004 L-SSE. However, the slip expanded in the northeast and southwest directions in the latter half of the second subevent. The maximum amount of slip, the maximum slip velocity, the total released seismic moment, and the moment magnitude of the 2018–2019 L-SSE were estimated to be 28 cm, 54 cm/year, $$4.4 \times 10^{19}$$

4.4
×

10
19

Nm, and 7.0, respectively, all of which were the largest among the 1996–1998, 2002–2004, 2009–2011, and 2018–2019 L-SSEs.
In vitro bioprocessing of corn as poultry feed additive by the influence of carbohydrate hydrolyzing metagenome derived enzyme cocktail
Authors: Mousavi Seyed Hossein , Sadeghian Motahar Seyedeh Fatemeh , Salami Maryam , Kavousi Kaveh , Sheykh Abdollahzadeh Mamaghani Atefeh , Ariaeenejad Shohreh , Salekdeh Ghasem Hosseini 
Published: January 2022

AbstractThe carbohydrate-hydrolyzing enzymes play a crucial role in increasing the phenolic content and nutritional properties of polysaccharides substrate, essential for cost-effective industrial applications. Also, improving the feed efficiency of poultry is essential to achieve significant economic benefits. The current study introduced a novel thermostable metagenome-derived xylanase named PersiXyn8 and investigated its synergistic effect with previously reported α-amylase (PersiAmy3) to enhance poultry feed utilization. The potential of the enzyme cocktail in the degradation of poultry feed was analyzed and showed 346.73 mg/g poultry feed reducing sugar after 72 h of hydrolysis. Next, the impact of solid-state fermentation on corn quality was investigated in the presence and absence of enzymes. The phenolic content increased from 36.60 mg/g GAE in control sample to 68.23 mg/g in the presence of enzymes. In addition, the enzyme-treated sample showed the highest reducing power OD 700 of 0.217 and the most potent radical scavenging activity against ABTS (40.36%) and DPPH (45.21%) radicals. Moreover, the protein and ash contents of the fermented corn increased by 4.88% and 6.46%, respectively. These results confirmed the potential of the carbohydrate-hydrolyzing enzymes cocktail as a low-cost treatment for improving the phenolic content, antioxidant activity, and nutritional values of corn for supplementation of corn-based poultry feed.
Genome survey sequencing and characterization of simple sequence repeat (SSR) markers in Platostoma palustre (Blume) A.J.Paton (Chinese mesona)
Authors: Zheng Zhao , Zhang Nannan , Huang Zhenghui , Zeng Qiaoying , Huang Yonghong , Qi Yongwen 
Published: January 2022

AbstractPlatostoma palustre (Blume) A.J.Paton is an annual herbaceous persistent plant of the Labiatae family. However, there is a lack of genomic data for this plant, which severely restricts its genetic improvement. In this study, we performed genome survey sequencing of P. palustre and developed simple sequence repeat (SSR) markers based on the resulting sequence. K-mer analysis revealed that the assembled genome size was approximately 1.21 Gb. A total of 15,498 SSR motifs were identified and characterized in this study; among them, dinucleotide, and hexanucleotide repeats had the highest and lowest, respectively. Among the dinucleotide repeat motifs, AT/TA repeat motifs were the most abundant, and GC/CG repeat motifs were rather rare, accounting for 44.28% and 0.63%, respectively. Genetic similarity coefficient analysis by the UPMGA methods clustered 12 clones, of P. palustre and related species into two subgroups. These results provide helpful information for further research on P. palustre resources and variety improvements.
3D-printed NiFe-layered double hydroxide pyramid electrodes for enhanced electrocatalytic oxygen evolution reaction
Authors: Ahn Jinhyuck , Park Yoo Sei , Lee Sanghyeon , Yang Juchan , Pyo Jaeyeon , Lee Jooyoung , Kim Geul Han , Choi Sung Mook , Seol Seung Kwon 
Published: January 2022

AbstractElectrochemical water splitting has been considered one of the most promising methods of hydrogen production, which does not cause environmental pollution or greenhouse gas emissions. Oxygen evolution reaction (OER) is a significant step for highly efficient water splitting because OER involves the four electron transfer, overcoming the associated energy barrier that demands a potential greater than that required by hydrogen evolution reaction. Therefore, an OER electrocatalyst with large surface area and high conductivity is needed to increase the OER activity. In this work, we demonstrated an effective strategy to produce a highly active three-dimensional (3D)-printed NiFe-layered double hydroxide (LDH) pyramid electrode for OER using a three-step method, which involves direct-ink-writing of a graphene pyramid array and electrodeposition of a copper conducive layer and NiFe-LDH electrocatalyst layer on printed pyramids. The 3D pyramid structures with NiFe-LDH electrocatalyst layers increased the surface area and the active sites of the electrode and improved the OER activity. The overpotential (η) and exchange current density (i0) of the NiFe-LDH pyramid electrode were further improved compared to that of the NiFe-LDH deposited Cu (NiFe-LDH/Cu) foil electrode with the same base area. The 3D-printed NiFe-LDH electrode also exhibited excellent durability without potential decay for 60 h. Our 3D printing strategy provides an effective approach for the fabrication of highly active, stable, and low-cost OER electrocatalyst electrodes.
Classifying central serous chorioretinopathy subtypes with a deep neural network using optical coherence tomography images: a cross-sectional study
Authors: Yoon Jeewoo , Han Jinyoung , Ko Junseo , Choi Seong , Park Ji In , Hwang Joon Seo , Han Jeong Mo , Jang Kyuhwan , Sohn Joonhong , Park Kyu Hyung , Hwang Daniel Duck-Jin 
Published: January 2022

AbstractCentral serous chorioretinopathy (CSC) is the fourth most common retinopathy and can reduce quality of life. CSC is assessed using optical coherence tomography (OCT), but deep learning systems have not been used to classify CSC subtypes. This study aimed to build a deep learning system model to distinguish CSC subtypes using a convolutional neural network (CNN). We enrolled 435 patients with CSC from a single tertiary center between January 2015 and January 2020. Data from spectral domain OCT (SD-OCT) images of the patients were analyzed using a deep CNN. Five-fold cross-validation was employed to evaluate the model’s ability to discriminate acute, non-resolving, inactive, and chronic atrophic CSC. We compared the performances of the proposed model, Resnet-50, Inception-V3, and eight ophthalmologists. Overall, 3209 SD-OCT images were included. The proposed model showed an average cross-validation accuracy of 70.0% (95% confidence interval [CI], 0.676–0.718) and the highest test accuracy was 73.5%. Additional evaluation in an independent set of 104 patients demonstrated the reliable performance of the proposed model (accuracy: 76.8%). Our model could classify CSC subtypes with high accuracy. Thus, automated deep learning systems could be useful in the classification and management of CSC.
The prevalence and distribution of the flexor carpi radialis brevis muscle in the Turkish population
Authors: Akkoc R. F. , Aksu F. , Emre E. , Ogeturk M. 
Published: January 2022

AbstractThe flexor carpi radialis brevis (FCRB) is a very rare anomalous muscle that is usually asymptomatic but may cause various pathologies, such as radial-sided wrist pain. The aim of this study was to determine the prevalence of FCRB in the Turkish population, its location, and sex differences. Forearm, wrist, and hand magnetic resonance images of 849 individuals aged 18–65 years were retrospectively evaluated in this study. The survey found an FCRB prevalence of 4%, with a prevalence of 3.6% among women and of 4.7% among men. However, the difference between the sexes was not statistically significant (p = 0.629). The origin of all 34 FCRBs identified was the distal third of the anterior aspect of the radius; the insertion site of 28 was the second metacarpal bone, whereas that of the remaining 6 was the os trapezium. In conclusion, the data of this study report the prevalence of FCRB for the first time in the Turkish population, which will contribute to radiological and surgical evaluations in the region and help in early and accurate diagnosis of various pathological conditions that may be caused by FCRB.
Growth at the limits: comparing trace metal limitation of a freshwater cyanobacterium (Dolichospermum lemmermannii) and a freshwater diatom (Fragilaria crotonensis)
Authors: Dengg Markus , Stirling Claudine H. , Reid Malcolm R. , Verburg Piet , Armstrong Evelyn , Kelly Laura T. , Wood Susanna A. 
Published: January 2022

AbstractFreshwater phytoplankton blooms are increasing in prevalence and there are conflicting views on whether trace metals limit growth of key species and thus bloom formation. The Taupō Volcanic Zone (TVZ), New Zealand, was formed by multiple eruptions of a super-volcano which emitted rhyolitic tephra leaving lakes depleted in trace metals. This provides an opportunity to test the potential of trace metal limitation on freshwater phytoplankton growth under nanomolar concentrations. Growth responses of two algal species isolated from Lake Taupō,Dolichospermum lemmermannii(cyanobacteria) andFragilaria crotonensis(diatom), to six biologically important trace metals (manganese, iron, zinc, cobalt, copper and molybdenum) were examined in culture experiments. These were conducted at three trace metal concentrations: (1) ambient, (2) two-times ambient, and (3) ten-times ambient concentrations in Lake Taupō. Elevated concentrations of iron significantly increased growth rates and maximum cell densities inD. lemmermannii,whereas no significant concentration dependence was observed for other trace metals.Fragilaria crotonensisshowed no significant growth response to elevated concentrations of trace metals. These results highlight the importance of iron as a growth limiting nutrient for cyanobacteria and indicate that even small (twofold) increases in Fe concentrations could enhance cyanobacteria growth rates in Lake Taupō, potentially causing cyanobacterial blooms.
IL-1β promotes hypoxic vascular endothelial cell proliferation through the miR-24-3p/NKAP/NF-κB axis
Authors: Huang Jiangnan , Li Yumei , Jiang Zhiyuan , Wu Lingjun , Liu Yueying , Ma Siwen , Li Lang , Wang Hui 
Published: January 2022

Abstract
Purpose: Our previous data indicated that miR-24-3p is involved in the regulation of vascular endothelial cell (EC) proliferation and migration/invasion. However, whether IL-1β affects hypoxic HUVECs by miR-24-3p is still unclear. Therefore, the present study aimed to investigate the role and underlying mechanism of interleukin 1β (IL-1β) in hypoxic HUVECs.
Methods: We assessed the mRNA expression levels of miR-24-3p, hypoxia-inducible factor-1α (HIF1A) and NF-κB-activating protein (NKAP) by quantitative real-time polymerase chain reaction (RT-qPCR). ELISA measured the expression level of IL-1β. Cell counting kit-8 (CCK-8) assays evaluated the effect of miR-24-3p or si-NKAP+miR-24 on cell proliferation (with or without IL-1β). Transwell migration and invasion assays were used to examine the effects of miR-24-3p or si-NKAP+miR-24-3p on cell migration and invasion (with or without IL-1β). Luciferase reporter assays were used to identify the target of miR-24-3p.
Results: We demonstrated that in acute myocardial infarction (AMI) patient blood samples, the expression of miR-24-3p is down-regulated, the expression of IL-1β or NKAP is up-regulated, and IL-1β or NKAP is negatively correlated with miR-24-3p. Furthermore, IL-1β promotes hypoxic HUVECs proliferation by down-regulating miR-24-3p. In addition, IL-1β also significantly promotes the migration and invasion of hypoxic HUVECs; overexpression of miR-24-3p can partially rescue hypoxic HUVECs migration and invasion. Furthermore, we discovered that NKAP is a novel target of miR-24-3p in hypoxic HUVECs. Moreover, both the overexpression of miR-24-3p and the suppression of NKAP can inhibit the NF-κB/pro-IL-1β signaling pathway. However, IL-1β mediates suppression of miR-24-3p activity, leading to activation of the NKAP/NF-κB pathway. In conclusion, our results reveal a new function of IL-1β in suppressing miR-24-3p up-regulation of the NKAP/NF-κB pathway.
Atomic diffusion in solar-like stars with MESA
Authors: Campilho B. , Deal M. , Bossini D. 
Published: January 2022

Context. The stellar evolution code Modules for Experiments in Stellar Astrophysics (MESA) is public and is widely used by the community. It includes the possibility of taking several non-standard processes such as atomic diffusion into account. Even if the effect of gravitational settling is considered a standard ingredient in stellar modelling today, this is not the case for radiative accelerations. The specific treatment of atomic diffusion along with the radiative accelerations has never been compared with other stellar evolution codes. Benchmarking these codes is important because improved accuracy is required in order to analyse data from present and future space missions, such as the Kepler, Transiting Exoplanet Survey Satellite, and PLAnetary Transits and Oscillations of stars missions.
Aims. The aim of this paper is to compare MESA models including atomic diffusion (with radiative accelerations) with models computed with the Montreal/Montpellier stellar evolution code and with the Code d’Evolution Stellaire Adaptatif et Modulaire (CESTAM). Additionally, we assess the impact of some MESA options related to atomic diffusion.
Methods. We calculated atomic diffusion, including radiative accelerations, following the abundance profiles of 14 elements with MESA models. This was then compared with 1.1 and 1.4 M⊙ models computed with the Montreal/Montpellier and CESTAM codes. Various tests of MESA options for atomic diffusion were also carried out by varying only one of them at a time.
Results. We find that the abundance profiles of the considered elements in the MESA models compare rather well with the models computed with the two other codes when atomic diffusion options are carefully set. We also show that some options in MESA are crucial for a proper treatment of atomic diffusion.
Experimental study of gas diffusion layers nonlinear orthotropic behavior
Authors: Ouerghemmi Marwa , Carral Christophe , Mele Patrice 
Published: January 2022

One of the most important components of PEMFC is the gas diffusion layer (GDL), owing to its key role in the reactant diffusion, water management, thermal and electron conductivity. Therefore, the GDL must have an optimal stiffness to ensure these transport functions during numerous hydrothermal cycles.
The understanding of its behavior is still a remaining issue. Its orthotropic mechanical behavior requires a series of mechanical characterizations in the plane of the fibers and out of plane. In addition, there are different manufacturing processes for GDL in sheet or roll form to optimize its functional properties. A macro porous layer (MPL) or different PTFE contents might be added by different manufacturers to optimize its performance. In this study, we have performed several mechanical tests differentiating between in plane and out of plane properties in order to characterize different GDLs available on the market. All of the experimental work has been done in the machine (MD) and cross machine direction (CD) according to the fiber orientation. The different GDL types were then classified into categories presenting similar mechanical response.
Expression of Prostate Specific Membrane Antigen (PSMA) in Breast Cancer
Authors: Unger Clara , Bronsert Peter , Michalski Kerstin , Bicker Anna , Juhasz-Böss Ingolf 
Published: January 2022

Abstract
Background Prostate specific membrane antigen (PSMA) is a promising protein for breast cancer patients. It has not only been detected in prostate cancer but is also expressed by tumor cells and the endothelial cells of tumor vessels in breast cancer patients. PSMA plays a role in tumor progression and tumor angiogenesis. For this reason, a number of diagnostic and therapeutic methods to target PSMA have been developed.
Method This paper provides a general structured overview of PSMA and its oncogenic potential, with a special focus on its role in breast cancer. This narrative review is based on a selective literature search carried out in PubMed and the library of Freiburg University Clinical Center. The following key words were used for the search: “PSMA”, “PSMA and breast cancer”, “PSMA PET/CT”, “PSMA tumor progression”. Relevant articles were explicitly read through, processed, and summarized.
Conclusion PSMA could be a new diagnostic and therapeutic alternative, particularly for triple-negative breast cancer. It appears to be a potential predictive and prognostic marker.