bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA MIR205HG accelerates cell proliferation, migration and invasion in hepatoblastoma through the activation of MAPK signaling pathway and PI3K/AKT signaling pathway
Authors: Zhang Wei , Liang Feng , Li Qingfeng , Sun Hong , Li Fei , Jiao Zhibo , Lei Jie 
Published: January 2022

AbstractBackgroundHepatoblastoma (HB) is identified to be the most common liver malignancy which occurs in children. Long non-coding RNAs (lncRNAs) have been implicated in numerous biological processes and diseases, including HB. LncRNA MIR205 host gene (MIR205HG) has been investigated in multiple cancers, however, its role in HB remains to be elucidated.MethodsMIR205HG expression was analyzed by RT-qPCR. EdU, colony formation and transwell assays were implemented to measure the biological function of MIR205HG on the progression of HB. Mechanism assays were carried out to probe into the underlying mechanism of MIR205HG in HB cells.ResultsMIR205HG was significantly overexpressed in HB. Moreover, MIR205HG inhibition suppressed the proliferative, migratory and invasive capacities of HB cells. Furthermore, MIR205HG competitively bound to microRNA-514a-5p (miR-514a-5p) and targeted mitogen-activated protein kinase 9 (MAPK9) to stimulate mitogen activated protein kinase (MAPK) signaling pathway. Besides, MIR205HG also served as a sponge for microRNA-205-5p (miR-205-5p) to activate the PI3K/AKT signaling pathway.ConclusionMIR205HG drives the progression of HB which might provide an efficient marker and new therapeutic target for HB.
Awake prone positioning in nonintubated spontaneous breathing ICU patients with acute hypoxemic respiratory failure (PRONELIFE)—protocol for a randomized clinical trial
Authors: Morales-Quinteros L. , Schultz M. J. , Serpa-Neto A. , Antonelli M. , Grieco D. L. , Roca O. , Juffermans N. P. , de Haro C. , de Mendoza D. , Blanch Ll. , Camprubí-Rimblas M. , Gomà Gemma , Artigas-Raventós A. ,   for the PRONELIFE collaborative group
Published: January 2022

Abstract
Background
It is uncertain whether awake prone positioning can prevent intubation for invasive ventilation in spontaneous breathing critically ill patients with acute hypoxemic respiratory failure. Awake prone positioning could benefit these patients for various reasons, including a reduction in direct harm to lung tissue, and prevention of tracheal intubation-related complications.

Design and methods
The PRONELIFE study is an investigator-initiated, international, multicenter, randomized clinical trial in patients who may need invasive ventilation because of acute hypoxemic respiratory failure. Consecutive patients admitted to participating ICUs are randomly assigned to standard care with awake prone positioning, versus standard care without awake prone positioning. The primary endpoint is a composite of tracheal intubation and all-cause mortality in the first 14 days after enrolment. Secondary endpoints include time to tracheal intubation and effects of awake prone positioning on oxygenation parameters, dyspnea sensation, and complications. Other endpoints are the number of days free from ventilation and alive at 28 days, total duration of use of noninvasive respiratory support, total duration of invasive ventilation, length of stay in ICU and hospital, and mortality in ICU and hospital, and at 28, 60, and 90 days. We will also collect data regarding the tolerance of prone positioning.

Discussion
The PRONELIFE study is among the first randomized clinical trials investigating the effect of awake prone positioning on intubation rate in ICU patients with acute hypoxemic failure from any cause. The PRONELIFE study is sufficiently sized to determine the effect of awake prone positioning on intubation for invasive ventilation—patients are eligible in case of acute hypoxemic respiratory failure without restrictions regarding etiology. The PRONELIFE study is a pragmatic trial in which blinding is impossible—however, as around 35 ICUs worldwide will participate in this study, its findings will be highly generalizable. The findings of the PRONELIFE study have the potential to change clinical management of patients who may need invasive ventilation because of acute hypoxemic respiratory failure.

Trial registration
ISRCTN ISRCTN11536318. Registered on 17 September 2021. The PRONELIFE study is registered at clinicaltrials.gov with reference number NCT04142736 (October, 2019).
Performance evaluation and comparison study of adaptive MANET service location and discovery protocols for highly dynamic environments
Authors: Ben Brahim Ghassen , Mohammad Nazeeruddin , El-Hajj Wassim , Parr Gerard , Scotney Bryan 
Published: January 2022

AbstractA critical requirement in Mobile Ad Hoc Networks (MANETs) is its ability to automatically discover existing services as well as their locations. Several solutions have been proposed in various communication domains which could be classified into two categories: (1) directory based, and (2) directory-less. The former is efficient but suffers from the amount of control messages being exchanged to maintain all directories in an agile environment. However, the latter approach attempts to reduce the amount of control messages to update directories, by simply sending broadcast messages to discover services; which is also a non-desirable approach in MANETs. This research work builds on top of our prior work (Nazeeruddin et al. in IFIP/IEEE international conference on management of multimedia networks and services, Springer, Berlin, 2006)) where we introduced a new efficient protocol for service discovery in MANETs (MSLD); a lightweight, robust, scalable, and flexible protocol which supports node heterogeneity and dynamically adapts to network changes while not flooding the network with extra protocol messages—a major challenge in today’s network environments, such as Internet of Things (IoT). Extensive simulations study was conducted on MSLD to: (1) initially evaluate its performance in terms of latency, service availability, and overhead messages, then (2) compare its performance to Dir-Based, Dir-less, and PDP protocols under various network conditions. For most performance metrics, simulation results show that MSLD outperforms Dir-Based, Dir-less, and PDP by either matching or achieving high service availability, low service discovery latency, and considerably less communication overhead.
Two efficient modifications of AZPRP conjugate gradient method with sufficient descent property
Authors: Salleh Zabidin , Almarashi Adel , Alhawarat Ahmad 
Published: January 2022

AbstractThe conjugate gradient method can be applied in many fields, such as neural networks, image restoration, machine learning, deep learning, and many others. Polak–Ribiere–Polyak and Hestenses–Stiefel conjugate gradient methods are considered as the most efficient methods to solve nonlinear optimization problems. However, both methods cannot satisfy the descent property or global convergence property for general nonlinear functions. In this paper, we present two new modifications of the PRP method with restart conditions. The proposed conjugate gradient methods satisfy the global convergence property and descent property for general nonlinear functions. The numerical results show that the new modifications are more efficient than recent CG methods in terms of number of iterations, number of function evaluations, number of gradient evaluations, and CPU time.
Homologous tumor cell membrane vesicles active preferential self-recognition of tumor cells in vitro
Authors: Wu Chenghu , Yu Ailin , Chen Yue , Fan Mingbo 
Published: January 2022

AbstractCell membrane vesicles, as delivery carriers of drugs or biological agents in vivo, are an important therapeutic mode in the study of disease treatment. Tumor membrane-derived vesicles have been widely used in tumor therapy because of their good tumor enrichment effect. The most common method is the surface of nanoparticles coated with tumor cell membrane, which can effectively prolong the circulation time of particles in the blood and the enrichment of tumors. In this study, we prepared vesicles of different tumor cell membrane derivate and studied their targeting to tumors detailly. The results showed that homologous vesicles have high targeting to homologous tumor cells. The fluorescence of vesicles in homologous tumor cells was significantly higher than that in other tumor cells. This study will provide a new strategy and guidance for the clinical treatment of cancer based on the tumor cell membrane system.Graphical Abstract
Pleomorphic xanthoastrocytoma is a heterogeneous entity with pTERT mutations prognosticating shorter survival
Authors: Ebrahimi Azadeh , Korshunov Andrey , Reifenberger Guido , Capper David , Felsberg Joerg , Trisolini Elena , Pollo Bianca , Calatozzolo Chiara , Prinz Marco , Staszewski Ori , Schweizer Leonille , Schittenhelm Jens , Harter Patrick N. , Paulus Werner , Thomas Christian , Kohlhof-Meinecke Patricia , Seiz-Rosenhagen Marcel , Milde Till , Casalini Belén M. , Suwala Abigail , Wefers Annika K. , Reinhardt Annekathrin , Sievers Philipp , Kramm Christof M. , Etminam Nima , Unterberg Andreas , Wick Wolfgang , Herold-Mende Christel , Sturm Dominik , Pfister Stefan M. , Sill Martin , Jones David T. W. , Schrimpf Daniel , Reuss David E. , Aldape Ken , Abdullaev Zied , Sahm Felix , von Deimling Andreas , Stichel Damian 
Published: January 2022

AbstractPleomorphic xanthoastrocytoma (PXA) in its classic manifestation exhibits distinct morphological features and is assigned to CNS WHO grade 2 or grade 3. Distinction from glioblastoma variants and lower grade glial and glioneuronal tumors is a common diagnostic challenge. We compared a morphologically defined set of PXA (histPXA) with an independent set, defined by DNA methylation analysis (mcPXA). HistPXA encompassed 144 tumors all subjected to DNA methylation array analysis. Sixty-two histPXA matched to the methylation class mcPXA. These were combined with the cases that showed the mcPXA signature but had received a histopathological diagnosis other than PXA. This cohort constituted a set of 220 mcPXA. Molecular and clinical parameters were analyzed in these groups. Morphological parameters were analyzed in a subset of tumors with FFPE tissue available. HistPXA revealed considerable heterogeneity in regard to methylation classes, with methylation classes glioblastoma and ganglioglioma being the most frequent mismatches. Similarly, the mcPXA cohort contained tumors of diverse histological diagnoses, with glioblastoma constituting the most frequent mismatch. Subsequent analyses demonstrated the presence of canonical pTERT mutations to be associated with unfavorable prognosis among mcPXA. Based on these data, we consider the tumor type PXA to be histologically more varied than previously assumed. Histological approach to diagnosis will predominantly identify cases with the established archetypical morphology. DNA methylation analysis includes additional tumors in the tumor class PXA that share similar DNA methylation profile but lack the typical morphology of a PXA. DNA methylation analysis also assist in separating other tumor types with morphologic overlap to PXA. Our data suggest the presence of canonical pTERT mutations as a robust indicator for poor prognosis in methylation class PXA.
Investigating the effect of bruxism on maxillary arch length and width in children using three-dimensional digital model analysis
Authors: Hazar Bodrumlu Ebru , Çakmak Özlü Fethiye , Yılmaz Hakan , Demiriz Levent 
Published: January 2022

Abstract
Background
Bruxism is defined as repetitive jaw-muscle activity characterized by the grinding and clenching of teeth. The prevalence of bruxism in children is extensive, and it can cause irregularities in dental arches. The study aimed to investigate the presence of any effects of bruxism on maxillary arch length and width in children using three-dimensional (3D) digital model analysis.

Method
This study evaluated 30 children with bruxism. For every child with bruxism, a case control without bruxism was selected and matched for gender, age, and dentition. Digital models of the patients’ maxilla were obtained with a 3D intraoral scanner, and width and length measurements between the reference points on the maxilla were obtained on the digital models.

Results
The mean age of the study group was 9.13 ± 1.27. Insıgnificance differences were found between females and males within and between groups in terms of maxillary width and length. Insignificant difference was found between the control and study groups when the lengths of 3R-3L, 4R-4L, 5R-5L, 6R-6L, and IP-M were compared (p > 0.05).

Conclusion
Based on the study results, there were no differences in the maxillary arch length and width in patients with bruxism and patients without bruxism.
Anterior cruciate ligament reconstruction, can an anatomic femoral tunnel be achieved with the trans-tibial technique? Cadaveric study
Authors: Acevedo Tobler Daniel , Hermosilla Santiago , Otero Natalia , Kenny Juan , Del Castillo Juan 
Published: January 2022

Abstract
Purpose
To evaluate the possibility to access the anatomic femoral insertion of the anterior cruciate ligament (ACL) through trans-tibial (TT) and trans-portal technique, for ACL reconstruction in an independent way. To register anatomical characteristics of the TT tunnels.

Methods
Ten formaldehyde preserved knee anatomic articular specimens were dissected. Femoral tunnels were confectioned reproducing the original topography of the ACL. First, the femoral tunnel was made with the independent trans-portal technique. Then, utilizing the tibial stump of the ACL and tibial guides at 45°, the TT tunnels were confectioned trying to match the previously made femoral tunnel by trans-portal technique.

Results
In all specimens, match between the TT tunnel with the independent trans-portal tunnel was achieved. Mean values for TT coronal angle was 53,0°, for transversal angle 43,3° and for distance from tunnel to joint line 2,55 cm. A horizontalization and medialization of the TT tunnels had to be made to adequately match with the femoral tunnel made by the independent trans-portal technique.

Conclusions
By macroscopic anatomic and independent means, an anatomic femoral tunnel was confectioned with the TT technique matching with the anatomic femoral tunnel made in an independent way. As clinical relevance, the present study allows to anatomically assess the possibility to perform an anatomic femoral tunnel through the TT technique.

Level of evidence
V
The colors of life: an interdisciplinary artist-in-residence project to research fungal pigments as a gateway to empathy and understanding of microbial life
Authors: Sharma Sunanda , Meyer Vera 
Published: January 2022

Abstract
Background
Biological pigmentation is one of the most intriguing traits of many fungi. It holds significance to scientists, as a sign of biochemical metabolism and organism-environment interaction, and to artists, as the source of natural colors that capture the beauty of the microbial world. Furthermore, the functional roles and aesthetic appeal of biological pigmentation may be a path to inspiring human empathy for microorganisms, which is key to understanding and preserving microbial biodiversity. A project focused on cross-species empathy was initiated and conducted as part of an artist-in-residence program in 2021. The aim of this residency is to bridge the current divide between science and art through interdisciplinary practice focused on fungi.

Results
The residency resulted in multiple products that are designed for artistic and scientific audiences with the central theme of biological pigmentation in fungi and other microorganisms. The first product is a video artwork that focuses on Aspergillus niger as a model organism that produces melanin pigment in a biosynthetic process similar to that of humans. The growth and morphology of this commonplace organism are displayed through video, photo, animation, and time-lapse footage, inviting the viewer to examine the likenesses and overlaps between humans and fungi. The second product is The Living Color Database, an online compendium of biological colors for scientists, artists, and designers. It links organisms across the tree of life, focusing on fungi, bacteria, and archaea, and the colors they express through biological pigmentation. Each pigment is represented in terms of its chemistry, its related biosynthesis, and its color expressions according to different indices: HEX, RGB, and Pantone. It is available at color.bio.

Conclusions
As fungal biotechnology continues to mature into new application areas, it is as important as ever that there is human empathy for these organisms to promote the preservation and appreciation of fungal biodiversity. The products presented here provide paths for artists, scientists, and designers to understand microorganisms through the lens of color, promoting interspecies empathy through research, teaching, and practice.
Towards a better understanding of antimicrobial resistance dissemination: what can be learnt from studying model conjugative plasmids?
Authors: Shen Zhen , Tang Christoph M. , Liu Guang-Yu 
Published: January 2022

AbstractBacteria can evolve rapidly by acquiring new traits such as virulence, metabolic properties, and most importantly, antimicrobial resistance, through horizontal gene transfer (HGT). Multidrug resistance in bacteria, especially in Gram-negative organisms, has become a global public health threat often through the spread of mobile genetic elements. Conjugation represents a major form of HGT and involves the transfer of DNA from a donor bacterium to a recipient by direct contact. Conjugative plasmids, a major vehicle for the dissemination of antimicrobial resistance, are selfish elements capable of mediating their own transmission through conjugation. To spread to and survive in a new bacterial host, conjugative plasmids have evolved mechanisms to circumvent both host defense systems and compete with co-resident plasmids. Such mechanisms have mostly been studied in model plasmids such as the F plasmid, rather than in conjugative plasmids that confer antimicrobial resistance (AMR) in important human pathogens. A better understanding of these mechanisms is crucial for predicting the flow of antimicrobial resistance-conferring conjugative plasmids among bacterial populations and guiding the rational design of strategies to halt the spread of antimicrobial resistance. Here, we review mechanisms employed by conjugative plasmids that promote their transmission and establishment in Gram-negative bacteria, by following the life cycle of conjugative plasmids.
Energetics of Floor Gymnastics: Aerobic and Anaerobic Share in Male and Female Sub-elite Gymnasts
Authors: Kaufmann Sebastian , Ziegler Martin , Werner Jana , Noe Christine , Latzel Richard , Witzany Stefan , Beneke Ralph , Hoos Olaf 
Published: January 2022

Abstract
Background
Artistic gymnastics is a popular Olympic discipline where female athletes compete in four and male athletes in six events with floor exercise having the longest competition duration in Women’s and Men’s artistic gymnastics (WAG, MAG). To date no valid information on the energetics of floor gymnastics is available although this may be important for specific conditioning programming. This study evaluated the metabolic profile of a simulated floor competition in sub-elite gymnasts.

Methods
17 (9 male, 8 female) sub-elite gymnasts aged 22.5 ± 2.6y took part in a floor-training-competition where oxygen uptake was measured during and until 15 min post-exercise. Additionally, resting and peak blood lactate concentration after exercise were obtained. The PCr-LA-O2 method was used to calculate the metabolic energy and the relative aerobic (WAER), anaerobic alactic (WPCr) and anaerobic lactic (WBLC) energy contribution. Further, the athletes completed a 30 s Bosco-jumping test, a countermovement jump and a drop jump.

Results
The competition scores were 9.2 (CI:8.9–9.3) in WAG and 10.6 (CI:10.4–10.9) in MAG. The metabolic profile of the floor routine was mainly aerobic (58.9%, CI: 56.0–61.8%) followed by the anaerobic alactic (24.2%, CI: 21.3–27.1%) and anaerobic lactic shares (16.9%, CI:14.9–18.8%). While sex had a significant (p = .010, d = 1.207) large effect on energy contribution, this was not the case for competition duration (p = .728, d = 0.061). Relative energy contribution of WAG and MAG differed in WAER (64.0 ± 4.7% vs. 54.4 ± 6.8%, p = .004, d = 1.739) but not in WPCr (21.3 ± 6.1% vs. 26.7 ± 8.0%, p = .144, d = 0.801) and WBLC (14.7 ± 5.4% vs. 18.9 ± 4.2%, p = .085, d = 0.954). Further no correlation between any energy share and performance was found but between WPCr and training experience (r = .680, p = .044) and WBLC and competition level (r = .668, p = .049).

Conclusion
The results show a predominant aerobic energy contribution and a considerable anaerobic contribution with no significant difference between anaerobic shares. Consequently, gymnastic specific aerobic training should not be neglected, while a different aerobic share in WAG and MAG strengthens sex-specific conditioning. All in all, the specific metabolic share must secure adequate energy provision, while relative proportions of the two anaerobic pathways seem to depend on training and competition history.
Inter-organisational communication: organisational future orientation, inter-organisational interaction quality and inter-organisational group mechanism
Authors: Mutebi Henry , Muhwezi Mose , Ntayi Joseph M. , Munene John C. 
Published: January 2022

AbstractThe purpose of this study is to investigate the effect of organisational future orientation and interaction quality on inter-organisational communication, and the mediating role of inter-organisational group mechanism on the relationship between organisational future orientation, interaction quality and inter-organisational communication. A questionnaire assessing the experience of organisational future orientation, inter-organisational interaction quality, inter-organisational group mechanism and inter-organisational communication was administered to 101 out of 136 humanitarian organisations delivering social services to refugee settlements in Uganda. The proposed hypotheses were tested using PLS-SEM in the SmartPLS version 3.3.0 for professionals. The paper found that organisational future orientation has a positive impact on inter-organisational communication. Inter-organisational group mechanism partially mediates organisational future orientation and inter-organisational communication whilst it fully mediates the relationship between inter-organisational interaction quality and inter-organisational communication. The findings of this research provide useful insights into the role of inter-organisational group mechanism in boosting the role of organisational future orientation and interaction quality in inter-organisational communication in humanitarian relief delivery. Hence, a high level of inter-organisational group mechanism not only improves inter-organisational communication in humanitarian relief delivery but also enhances the benefits of organisational future orientation and interaction quality for inter-organisational communication. This research is one of the limited studies that investigate the effect of organisation future orientation and interaction quality on inter-organisational group mechanism and inter-organisational communication in humanitarian relief delivery in sub-Saharan Africa.