bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing the meaningful change threshold of Quality of Life in Depression Scale using data from two phase 3 studies of esketamine nasal spray
Authors: Rozjabek Heather , Li Nan , Hartmann Holger , Fu Dong Jing , Canuso Carla , Jamieson Carol 
Published: July 2022

Abstract
Background
Major depressive disorder (MDD) directly impacts patients’ lives including symptoms, functioning and health-related quality-of-life (HRQoL). Patient-reported outcomes can capture these impacts, however interpretation of clinical meaningfulness of these measurements are often not readily available. Meaningful change thresholds (MCTs) can be derived for clinical outcome assessments to quantify the change in symptoms that is meaningful to the patient following pharmacologic treatment or other interventions. The objective of this analysis was to determine the within-patient MCT of the self-reported Quality-of-Life in Depression Scale (QLDS) among patients with MDD and active suicidal ideation with intent (MDSI) using an anchor-based approach.

Methods
Data from 2 randomized phase-3 trials of esketamine nasal spray (ASPIRE I and ASPIRE II) were analyzed. The Montgomery–Åsberg Depression Rating Scale (MADRS) was the primary anchor with three different severity criteria. Other anchor variables utilized were Clinical Global Impression of Severity of Suicidality-revised version, Clinical Global Impression of Imminent Suicide Risk, and EuroQol Visual Analog Scale [EQ-VAS]. Spearman correlation coefficients between the change in QLDS and anchor variables were calculated. The mean change in QLDS score at Day 25 from baseline was calculated based on the categorical change in the anchor. Coefficient yield from linear regression of the mean changes in EQ-VAS and QLDS, and distribution-based approach with ½ SD of change in QLDS were considered.

Results
In ASPIRE I, mean (SD) improvement in QLDS score among patients with one category improvement in MADRS from baseline to Day 25 was − 8.22 (8.87), − 8.30 (9.01), and − 8.20 (8.92) using severity criteria #1, #2, and #3, respectively. Patients who achieved a 7-point improvement (MCT) in EQ-VAS yielded a mean − 9.69-point improvement in QLDS at Day 25. The ½ SD of change in QLDS was 5.63. Similar results were obtained for ASPIRE II. The MCTs identified using multiple anchors across both trials ranged from − 11.4 to − 6.7 and had an overall mean of − 7.90 (ASPIRE I) and − 7.92 (ASPIRE II). Thus, an 8-point change was recommended as the MCT for QLDS.

Conclusion
The recommended MCT will help quantify within-person changes in HRQoL using patient-reported QLDS and determine meaningful treatment benefit in an MDD patient population with acute suicidal ideation or behavior.

Trial registration: Name of the registry: ClinicalTrials.gov. Trial registration number: ASPIRE I (NCT03039192), ASPIRE II (NCT03097133). Date of registration: February 01, 2017; March 31, 2017. URL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT03039192; https://clinicaltrials.gov/ct2/show/NCT03097133.
Dental arch form and arch dimensions among a group of Egyptian children and adolescents
Authors: Sharaf Rasha F. , Radwan Ehab , Salem Ghada A. , Abou El-yazeed Mohamed 
Published: July 2022

AbstractBackgroundGrowth and development of the dental arch is considered a continuous and complex biological process. During transition from primary to mixed then to permanent dentition, dental arch form, length and width vary, due to tooth movement and vertical growth of alveolar process. These naturally occurring changes, which happen in untreated individuals, have been used as a comparative standard to assist in diagnosis and treatment planning of children with malocclusion. Therefore, the aim of the current study was to analyze dental arch dimensions in Egyptian children and adolescents with normal occlusion in primary, mixed, and permanent dentitions as well as detection of the most common forms of the dental arches among a group of Egyptian children and adolescents.ResultsResults showed that lower and upper arch lengths showed increase during transition from mixed to permanent dentition. A small increase in lower anterior arch length was recorded during transition from primary (4.63) to mixed dentition (4.70), while reduction of upper anterior arch length was observed during transition from primary (8.35) to mixed dentitions. An increase in anterior arch length was observed in both arches during transition from mixed to permanent dentitions (from 4.70 to 5.42 in the mandible and from7.92 to 9.90 in the maxilla). Arch perimeter, intercanine width, intermolar width increased in the upper and lower arches during transition between dentition till reaching the permanent dentition. It was also found that the ovoid arch form (58%) and square arch form (29%) were the most prevalent among Egyptian children and adolescents followed by the tapered arch form (13%).ConclusionsDental arch measurements of Egyptian children and adolescents can be used as a guide for treatment planning of Egyptian children and can be used in further studies to provide standard values for the arch dimensions of the Egyptian children. Regarding the arch form, the ovoid arch form was the most common among the Egyptian population followed by the square while the tapered arch form was the least common one.
Modeled interactions of mountain pine beetle and wildland fire under future climate and management scenarios for three western US landscapes
Authors: Keane Robert E. , Bentz Barbara , Holsinger Lisa M. , Saab Victoria A. , Loehman Rachel 
Published: July 2022

Abstract
Background
Mountain pine beetle (MPB) is a native disturbance agent across most pine forests in the western US. Climate changes will directly and indirectly impact frequencies and severities of MPB outbreaks, which can then alter fuel characteristics and wildland fire dynamics via changes in stand structure and composition. To investigate the importance of MPB to past and future landscape dynamics, we used the mechanistic, spatially explicit ecosystem process model FireBGCv2 to quantify interactions among climate, MPB, wildfire, fire suppression, and fuel management under historical and projected future climates for three western US landscapes. We compared simulated FireBGCv2 output from three MPB modules (none, simple empirical, and complex mechanistic) using three focus variables and six exploratory variables to evaluate the importance of MPB to landscape dynamics.

Results
We found that inclusion of MPB (empirical or mechanistic) in the simulations significantly changed past and future landscape dynamics and that the mechanistic MPB module had more cross-scale interactions that increased variability, and perhaps realism, of simulation results. We also evaluated impacts of fire and fuel management on MPB dynamics and found that fire suppression influenced fuel loadings more than MPB disturbance, but at a landscape scale, most fuel treatment programs did little to change fuel loadings, MPB dynamics, and burned area, except under high fire suppression.

Conclusions
Synergistic interactions of climate, MPB, and wildfire catalyzed landscape-scale changes in vegetation distributions, fuels, and fire regimes in FireBGCv2 simulations. Models that simulate climate change on pine-dominated landscapes may be improved by including mechanistic MPB simulations to account for potentially important ecological interactions.
COVID-19 outcomes in hospitalized Parkinson’s disease patients in two pandemic waves in 2020: a nationwide cross-sectional study from Germany
Authors: Scherbaum Raphael , Bartig Dirk , Richter Daniel , Kwon Eun Hae , Muhlack Siegfried , Gold Ralf , Krogias Christos , Tönges Lars 
Published: July 2022

Abstract
Background
The individualized clinical and public health management of the COVID-19 pandemic have changed over time, including care of people with PD. The objective was to investigate whether in-hospital COVID-19 outcomes and hospital care utilization of people with PD differed between the first two pandemic waves (W) 2020 in Germany.

Methods
We conducted a nationwide cross-sectional study of inpatients with confirmed COVID-19 and PD between March 1 and May 31 (W1), and October 1 and December 31 (W2), 2020 and 2019, using an administrative database. Outcomes were in-hospital mortality, ICU admission rate, change in hospital care utilization, demographical data, PD clinical characteristics, and selected comorbidities. Differences were assessed between waves, PD/non-PD groups, and years.

Results
We identified 2600 PD COVID-19 inpatients in W2 who in total showed higher in-hospital mortality rates and lower ICU admission rates, compared to both W1 (n = 775) and W1/W2 non-PD COVID-19 inpatients (n = 144,355). Compared to W1, W2 inpatients were more long-term care-dependent, older, more of female sex, and had less advanced disease. During both waves, PD inpatients were older, more frequently male and long-term care-dependent, and showed more risk comorbidities than non-PD COVID-19 inpatients. Decreases in hospital care utilization were stronger than average for PD inpatients but relatively weaker during W2. Non-COVID-19 PD inpatients showed poorer in-hospital outcomes in 2020 than in 2019 with better outcomes during W2.

Conclusions
In-hospital COVID-19 outcomes and hospital care utilization of PD patients in Germany differed between the two pandemic waves in 2020 with increased in-hospital mortality for PD COVID-19. Overall hospital care utilization for PD was increased during W2.

Trial registration

No trial registration or ethical approval was required because data were publicly available, anonymized, and complied with the German data protection regulations.
An increase in the number of peroxisomes is coupled to the initial infection stage and stress response of Botrytis cinerea
Authors: Han Hongjia , Niu Xuejing , Liang Wenxing , Liu Mengjie 
Published: July 2022

AbstractPeroxisomes are very important organelles in eukaryotic cells and participate in various biological processes, including pathogen–host interactions. A variety of proteins involved in peroxisome proliferation and metabolic activity within peroxisomes have been shown to be essential for full virulence of several phytopathogenic fungi. However, the effects of changes in the number of peroxisomes and proteins involved in the peroxisome pathway on the pathogenicity of Botrytis cinerea have rarely been reported. In this study, by analysing transcriptome data and RT-qPCR validation, we found that more than half of the genes annotated to the peroxisome pathway in B. cinerea were upregulated more than twofold between mycelial samples cultured in medium with tomato leaves and without tomato leaves. A strain of B. cinerea with fluorescently labelled peroxisomes was obtained by overexpression of GFP fused to peroxisomal targeting signal 1 (the tripeptide ‘SKL’). The addition of tomato leaves to the liquid medium induced a significant increase in the number of peroxisomes, β-oxidation level, H2O2 content, and acetyl-CoA level in B. cinerea mycelia. When B. cinerea was cultured with oleic acid as the sole carbon source, the formation of infection-related structures and their penetration into plant cells were found to be associated with peroxisome pathway activity. Furthermore, peroxisome proliferation and lipid metabolism increased in response to different extracellular stresses in B. cinerea. Taken together, our results confirmed that activation of the peroxisome pathway in B. cinerea contributes to the initial infection and the ability to cope with environmental stress.
FramingThe Wealth of Nations
Authors: Tribe Keith 
Published: July 2022

AbstractWhen The Wealth of Nations was published in 1776, this was recognized as another work by the author of The Theory of Moral Sentiments, a work that Smith extended and revised through six editions until the year he died, in 1790. Smith had in 1776 refrained from elaborating arguments that he had already made in 1759, and this was likely well understood by his contemporaries. But by the later 1790s The Theory of Moral Sentiments was no longer so widely read and was becoming regarded as dated in its approach. Smith had located the nature and causes of the wealth of nations in human attributes and prudent policy; detached from the context provided by The Theory of Moral Sentiments, The Wealth of Nations was now treated as a repository of economic principles that required criticism and correction. It was quickly converted into the founding text of a new political economy, exemplified by the French translation by Garnier (1802), Jean-Baptiste Say's Traité (1803), and of course David Ricardo's Principles (1817). Political economists of the early nineteenth century disregarded most of those features of The Wealth of Nations that have since the later 1970s been reemphasized by new scholarship; that the Glasgow edition began publication for the 1976 bicentenary of The Wealth of Nations was at the time thought only fitting for a writer then treated as the property of economists. But by comparing Ricardo's Principles with Smith's Wealth of Nations we can better understand that this foreshortened appreciation of Adam Smith's work has a longer history than usually thought.
Lrig1- and Wnt-dependent niches dictate segregation of resident immune cells and melanocytes in murine tail epidermis
Authors: Baess Susanne C. , Burkhart Ann-Kathrin , Cappello Sabrina , Graband Annika , Seré Kristin , Zenke Martin , Niemann Catherin , Iden Sandra 
Published: July 2022

ABSTRACTThe barrier-forming, self-renewing mammalian epidermis comprises keratinocytes, pigment-producing melanocytes and resident immune cells as first-line host defense. In murine tail skin, interfollicular epidermis patterns into pigmented ‘scale’ and hypopigmented ‘interscale’ epidermis. Why and how mature melanocytes accumulate in scale epidermis is unresolved. Here, we delineate a cellular hierarchy among epidermal cell types that determines skin patterning. Already during postnatal development, melanocytes co-segregate with newly forming scale compartments. Intriguingly, this process coincides with partitioning of both Langerhans cells and dendritic epidermal T cells to interscale epidermis, suggesting functional segregation of pigmentation and immune surveillance. Analysis of non-pigmented mice and of mice lacking melanocytes or resident immune cells revealed that immunocyte patterning is melanocyte and melanin independent and, vice versa, immune cells do not control melanocyte localization. Instead, genetically enforced progressive scale fusion upon Lrig1 deletion showed that melanocytes and immune cells dynamically follow epithelial scale:interscale patterns. Importantly, disrupting Wnt-Lef1 function in keratinocytes caused melanocyte mislocalization to interscale epidermis, implicating canonical Wnt signaling in organizing the pigmentation pattern. Together, this work uncovers cellular and molecular principles underlying the compartmentalization of tissue functions in skin.
Foxl2 is required for the initiation of the female pathway in a temperature-dependent sex determination system in Trachemys scripta
Authors: Ma Xiaohui , Liu Fang , Chen Qiran , Sun Wei , Shen Jiadong , Wu Kaiyue , Zheng Ziyan , Huang Jiaqi , Chen Jiawen , Qian Guoying , Ge Chutian 
Published: July 2022

ABSTRACT
KDM6B-mediated epigenetic modification of the testicular regulator Dmrt1 has previously been identified as the primary switch of the male pathway in a temperature-dependent sex-determination (TSD) system; however, the molecular network of the female pathway has not yet been established. Here, we have functionally characterized for the first time an upstream regulator of the female pathway, the forkhead transcription factor FOXL2, in Trachemys scripta, a turtle species with a TSD system. FOXL2 exhibited temperature-dependent female-specific expression patterns before the onset of gonadal differentiation and was preferentially localized in ovarian somatic cells. Foxl2 responded rapidly to temperature shifts and estrogen. Importantly, forced expression of Foxl2 at the male-producing temperature led to male-to-female sex reversal, as evidenced by the formation of an ovary-like structure, and upregulation of the ovarian regulators Cyp19a1 and R-spondin1. Additionally, knockdown of Foxl2 caused masculinization at the female-producing temperature, which was confirmed by loss of the female phenotype, development of seminiferous tubules, and elevated expression of Dmrt1 and Sox9. Collectively, we demonstrate that Foxl2 expression is necessary and sufficient to drive ovarian determination in T. scripta, suggesting a crucial role of Foxl2 in female sex determination in the TSD system.
Cerebral malaria – modelling interactions at the blood–brain barrier in vitro
Authors: Adams Yvonne , Jensen Anja Ramstedt 
Published: July 2022

ABSTRACT
The blood–brain barrier (BBB) is a continuous endothelial barrier that is supported by pericytes and astrocytes and regulates the passage of solutes between the bloodstream and the brain. This structure is called the neurovascular unit and serves to protect the brain from blood-borne disease-causing agents and other risk factors. In the past decade, great strides have been made to investigate the neurovascular unit for delivery of chemotherapeutics and for understanding how pathogens can circumvent the barrier, leading to severe and, at times, fatal complications. One such complication is cerebral malaria, in which Plasmodium falciparum-infected red blood cells disrupt the barrier function of the BBB, causing severe brain swelling. Multiple in vitro models of the BBB are available to investigate the mechanisms underlying the pathogenesis of cerebral malaria and other diseases. These range from single-cell monolayer cultures to multicellular BBB organoids and highly complex cerebral organoids. Here, we review the technologies available in malaria research to investigate the interaction between P. falciparum-infected red blood cells and the BBB, and discuss the advantages and disadvantages of each model.
Active exploration of an environment drives the activation of the hippocampus–amygdala complex of domestic chicks
Authors: Morandi-Raikova Anastasia , Mayer Uwe 
Published: July 2022

ABSTRACT
In birds, like in mammals, the hippocampus critically mediates spatial navigation through the formation of a spatial map. This study investigates the impact of active exploration of an environment on the hippocampus of young domestic chicks. Chicks that were free to actively explore the environment exhibited a significantly higher neural activation (measured by c-Fos expression) compared with those that passively observed the same environment from a restricted area. The difference was limited to the anterior and the dorsolateral parts of the intermediate hippocampus. Furthermore, the nucleus taeniae of the amygdala showed a higher c-Fos expression in the active exploration group than in the passive observation group. In both brain regions, brain activation was correlated with the number of locations that chicks visited during the test. This suggests that the increase of c-Fos expression in the hippocampus is related to increased firing rates of spatially coding neurons. Furthermore, our study indicates a functional linkage of the hippocampus and nucleus taeniae of the amygdala in processing spatial information. Overall, with the present study, we confirm that in birds, like in mammals, hippocampus and amygdala functions are linked and likely related to spatial representations.
Association of dissection features and primary collateral circulation with ischemic stroke in patients with spontaneous internal carotid artery dissection: evaluated using vessel wall-MRI and MRA
Authors: Shrestha Srijana , Bao Han , Gu Heyi , Gao Chao , Zeng Yizhen , Xie Kaipeng , Shi Yixin , Zhao Lei , He Bo , Zhao Wei , Tang Zhiwei , Li Zongfang 
Published: July 2022

Objective: To study the ischemic stroke risk factors in spontaneous internal carotid artery dissection (ICAD) patients via analyzing the dissection features and primary collateral circulation using vessel wall-MRI and magnetic resonance angiography. Methods: ICAD patients who had undergone VW-MRI were included in this study. A total of 36 patients were included and divided into ICAD stroke (N = 23) and non-stroke (N = 13) group. Dissection imaging features [intramural hematoma (IMH), length of IMH, intimal flap, double lumen, intraluminal thrombus, degree of stenosis] and primary collateral status were analyzed. The primary collateral score (0–4) was evaluated based on presence of anterior communicating and ipsilateral anterior cerebral artery A1 segment (0–2) and ipsilateral posterior communicating artery (0–2). Results: There were no significant differences in dissection imaging features such as presence of double lumen, intimal flap, IMH, length of IMH and intraluminal thrombus between the two groups. Degree of stenosis and primary collateral score showed significant differences between the two groups. Conclusion: Both the poor primary collateral circulation and severe stenosis may play an important role in occurrence of ischemic stroke for spontaneous ICAD patients and good primary collateral circulation can help to reduce the incidence of infarction. Advances in knowledge: ICAD is one of the major causes of ischemic stroke. Early evaluation of the status of the Circle of Willis in ICAD patients by MRI may help to make treatment strategies and improve clinical outcome.
Detecting, extracting, and mapping of inland surface water using Landsat 8 Operational Land Imager: A case study of Pune district, India
Authors: Kulkarni Rushikesh , Khare Kanchan , Khanum Humera 
Published: July 2022

Background: Recent developments in optical satellite remote sensing have led to a new era in the detection of surface water with its changing dynamics. This study presents the creation of surface water inventory for a part of Pune district (an administrative area), in India using the Landsat 8 Operational Land Imager (OLI) and a multi spectral water indices method. Methods: A total of 13 Landsat 8 OLI cloud free images were analyzed for surface water detection. Modified Normalized Difference Water Index (MNDWI) spectral index method was employed to enhance the water pixels in the image. Water and non-water areas in the map were discriminated using the threshold slicing method with a trial and error approach. The accuracy analysis based on kappa coefficient and percentage of the correctly classified pixels was presented by comparing MNDWI maps with corresponding Joint Research Centre (JRC) Global Surface Water Explorer (GSWE) images. The changes in the surface area of eight freshwater reservoirs within the study area (Bhama Askhed, Bhatghar, Chaskaman, Khadakwasala, Mulashi, Panshet, Shivrata, and Varasgaon) for the year 2016 were analyzed and compared to GSWE time series water databases for accuracy assessment. The annual water occurrence map with percentage water occurrence on a yearly basis was also prepared. Results: The kappa coefficient agreement between MNDWI images and GSWE images is in the range of 0.56 to 0.96 with an average agreement of 0.82 indicating a strong level of agreement. Conclusions: MNDWI is easy to implement and is a sufficiently accurate method to separate water bodies from satellite images. The accuracy of the result depends on the clarity of image and selection of an optimum threshold method. The resulting accuracy and performance of the proposed algorithm will improve with implementation of automatic threshold selection methods and comparative studies for other spectral indices methods.