bluuebunny/crossref_metadata_2025_split huggingface dataset:
A reciprocal translocation involving Aspergillus nidulans snxAHrb1/Gbp2 and gyfA uncovers a new regulator of the G2–M transition and reveals a role in transcriptional repression for the setBSet2 histone H3-lysine-36 methyltransferase
Authors: James Steven W , Palmer Jonathan , Keller Nancy P , Brown Morgan L , Dunworth Matthew R , Francisco Sarah G , Watson Katherine G , Titchen Breanna , Achimovich Alecia , Mahoney Andrew , Artemiou Joseph P , Buettner Kyra G , Class Madelyn , Sydenstricker Andrew L , Anglin Sarah Lea 
Published: August 2022

Abstract
Aspergillus nidulans snxA, an ortholog of Saccharomyces cerevisiae Hrb1/Gbp2 messenger RNA shuttle proteins, is—in contrast to budding yeast—involved in cell cycle regulation, in which snxA1 and snxA2 mutations as well as a snxA deletion specifically suppress the heat sensitivity of mutations in regulators of the CDK1 mitotic induction pathway. snxA mutations are strongly cold sensitive, and at permissive temperature snxA mRNA and protein expression are strongly repressed. Initial attempts to identify the causative snxA mutations revealed no defects in the SNXA protein. Here, we show that snxA1/A2 mutations resulted from an identical chromosome I–II reciprocal translocation with breakpoints in the snxA first intron and the fourth exon of a GYF-domain gene, gyfA. Surprisingly, a gyfA deletion and a reconstructed gyfA translocation allele suppressed the heat sensitivity of CDK1 pathway mutants in a snxA+ background, demonstrating that 2 unrelated genes, snxA and gyfA, act through the CDK1–CyclinB axis to restrain the G2–M transition, and for the first time identifying a role in G2–M regulation for a GYF-domain protein. To better understand snxA1/A2-reduced expression, we generated suppressors of snxA cold sensitivity in 2 genes: (1) loss of the abundant nucleolar protein Nsr1/nucleolin bypassed the requirement for snxA and (2) loss of the Set2 histone H3 lysine36 (H3K36) methyltransferase or a nonmethylatable histone H3K36L mutant rescued hypomorphic snxA mutants by restoring full transcriptional proficiency, indicating that methylation of H3K36 acts normally to repress snxA transcription. These observations are in line with known Set2 functions in preventing excessive and cryptic transcription of active genes.
Research Centers Collaborative Network Workshop on Sex and Gender Differences in Aging
Authors: Brinkley Tina E , Stites Shana D , Hunsberger Holly C , Karvonen-Gutierrez Carrie A , Li Mengting , Shaaban C Elizabeth , Thorpe Roland J , Kritchevsky Stephen B 
Published: August 2022

Abstract
Aging affects men and women differently; however, the impact of sex and gender on the aging process is not well understood. Moreover, these 2 concepts are often conflated, which further contributes to a lack of clarity on this important issue. In an effort to better understand the relevance of sex and gender in aging research, the Research Centers Collaborative Network sponsored a 1.5-day conference on sex and gender differences in aging that brought together key thought leaders from the 6 National Institute on Aging center programs. The meeting included sessions on comparing males and females, pathophysiological differences, sex/gender in clinical care, and gender and health in the social context. Presenters from a wide array of disciplines identified opportunities for multidisciplinary research to address current gaps in the field and highlighted the need for a more systematic approach to understanding the how and why of sex/gender differences, as well as the health implications of these differences and the sex/gender biases that affect clinical treatment and outcomes. This article summarizes the proceedings of the workshop and provides several recommendations to move the field forward, such as better data collection tools to assess the intersection of sex and gender in epidemiological research; a life course perspective with attention to fetal/developmental origins and key life stages; innovative animal models to distinguish contributions from sex hormones versus sex chromosomes; and integration of sex/gender into teaching and clinical practice. Ultimately, successful implementation of these recommendations will require thoughtful investigations across the translational spectrum and increased collaborations among those with expertise in sex and gender differences.
Mutual influence in support seeking and provision behaviors during comforting conversations: a turn-level analysis
Authors: Rains Stephen A , Bonito Joseph A , Lutovsky Bethany R , Nemcova Katerina , Tsetsi Eric , Ashtaputre Anjali , Pavlich Corey A , Akers Chelsie 
Published: August 2022

Abstract
Mutual influence is central to prominent supportive communication theories but remains understudied. We conduct a turn-level analysis to investigate mutual influence in the unfolding nature of conversations among 334 stranger dyads discussing a personal problem. We examine how the types of messages produced by support seekers influence the immediate response from providers, and how that provider response impacts the next message produced by support seekers. Seeker use of approach behaviors and exonerating justifications were associated with higher levels of person centeredness in provider responses, and avoidance behaviors were associated with lower levels of provider person centeredness. Higher levels of provider person centeredness were associated with a greater likelihood of approach behaviors, exonerating justifications, and incriminating justifications and lower likelihood of avoidance behaviors from seekers. The results collectively suggest virtuous and vicious cycles in the messages produced by seekers and providers during supportive conversations.
The chromosome-level genome of Gypsophila paniculata reveals the molecular mechanism of floral development and ethylene insensitivity
Authors: Li Fan , Gao Yuan , Jin Chunlian , Wen Xiaohui , Geng Huaiting , Cheng Ying , Qu Haoyue , Liu Xing , Feng Shan , Zhang Fan , Ruan Jiwei , Yang Chunmei , Zhang Liangsheng , Wang Jihua 
Published: August 2022

Abstract
Gypsophila paniculata, belonging to the Caryophyllaceae of the Caryophyllales, is one of the most famous worldwide cut flowers. It is commonly used as dried flowers, whereas the underlying mechanism of flower senescence has not yet been addressed. Here, we present a chromosome-scale genome assembly for G. paniculata with a total size of 749.58 Mb. Whole-genome duplication signatures unveil two major duplication events in its evolutionary history: an ancient one occurring before the divergence of Caryophyllaceae and a more recent one shared with Dianthus caryophyllus. The integrative analyses combining genomic and transcriptomic data reveal the mechanisms regulating floral development and ethylene response of G. paniculata. The reduction of AGAMOUS expression probably caused by sequence polymorphism and the mutation in miR172 binding site of PETALOSA are associated with the double flower formation in G. paniculata. The low expression of ETHYLENE RESPONSE SENSOR (ERS) and the reduction of downstream ETHYLENE RESPONSE FACTOR (ERF) gene copy number collectively lead to the ethylene insensitivity of G. paniculata, affecting flower senescence and making it capable of making dried flowers. This study provides a cornerstone for understanding the underlying principles governing floral development and flower senescence, which could accelerate the molecular breeding of the Caryophyllaceae species.
FLOWERING LOCUS Tindel variants confer vernalization-independent and photoperiod-insensitive flowering of yellow lupin (Lupinus luteusL.)
Authors: Plewiński Piotr , Rychel-Bielska Sandra , Kozak Bartosz , Maureira-Butler Iván J , Iqbal Muhammad Munir , Nelson Matthew N , Książkiewicz Michał 
Published: August 2022

AbstractOngoing climate change has considerably reduced the seasonal window for crop vernalization, concurrently expanding cultivation area into northern latitudes with long-day photoperiod. To address these changes, cool season legume breeders need to understand molecular control of vernalization and photoperiod. A key floral transition gene integrating signals from these pathways is the Flowering locus T (FT). Here, a recently domesticated grain legume, yellow lupin (Lupinus luteus L.), was explored for potential involvement of FT homologues in abolition of vernalization and photoperiod requirements. Two FTa (LlutFTa1a and LlutFTa1b) and FTc (LlutFTc1 and LlutFTc2) homologues were identified and sequenced for two contrasting parents of a reference recombinant inbred line (RIL) population, an early-flowering cultivar Wodjil and a late-flowering wild-type P28213. Large deletions were detected in the 5′ promoter regions of three FT homologues. Quantitative trait loci were identified for flowering time and vernalization response in the RIL population and in a diverse panel of wild and domesticated accessions. A 2227 bp deletion found in the LlutFTc1 promoter was linked with early phenology and vernalization independence, whereas LlutFTa1a and LlutFTc2 indels with photoperiod responsiveness. Comparative mapping highlighted convergence of FTc1 indel evolution in two Old World lupin species, addressing both artificial selection during domestication and natural adaptation to short season environmental conditions. We concluded that rapid flowering in yellow lupin is associated with the de-repression of the LlutFTc1 homologue from the juvenile phase, putatively due to the elimination of all binding sites in the promoter region for the AGAMOUS-like 15 transcription factor.
Investigation ofBrassicaand its relative genomes in the post-genomics era
Authors: Wu Jian , Liang Jianli , Lin Runmao , Cai Xu , Zhang Lei , Guo Xinlei , Wang Tianpeng , Chen Haixu , Wang Xiaowu 
Published: August 2022

AbstractThe Brassicaceae family includes many economically important crop species, as well as cosmopolitan agricultural weed species. In addition, Arabidopsis thaliana, a member of this family, is used as a molecular model plant species. The genus Brassica is mesopolyploid, and the genus comprises comparatively recently originated tetrapolyploid species. With these characteristics, Brassicas have achieved the commonly accepted status of model organisms for genomic studies. This paper reviews the rapid research progress in the Brassicaceae family from diverse omics studies, including genomics, transcriptomics, epigenomics, and three-dimensional (3D) genomics, with a focus on cultivated crops. The morphological plasticity of Brassicaceae crops is largely due to their highly variable genomes. The origin of several important Brassicaceae crops has been established. Genes or loci domesticated or contributing to important traits are summarized. Epigenetic alterations and 3D structures have been found to play roles in subgenome dominance, either in tetraploid Brassica species or their diploid ancestors. Based on this progress, we propose future directions and prospects for the genomic investigation of Brassicaceae crops.
High throughput saliency-based quantification of grape powdery mildew at the microscopic level for disease resistance breeding
Authors: Qiu Tian , Underhill Anna , Sapkota Surya , Cadle-Davidson Lance , Jiang Yu 
Published: August 2022

Abstract
Imaging-based high throughput phenotyping (HTP) systems have demonstrated promising solutions to enhance genetic understanding of grapevine powdery mildew (PM) resistance and have accelerated PM-resistant cultivar breeding. The accuracy and throughput of extracting phenotypic traits from images are still the bottleneck of modern HTP systems, especially at the microscopic level. The goal of this study was to develop a saliency-based processing pipeline for the quantification of PM infection in microscopic images and comprehensively evaluate its performance for genetic analyses. An input image was segregated into subimages that were classified as infected or healthy by a pretrained CNN classifier. Saliency maps from the classification were generated post-hoc and used for the quantification of PM infection in the input image at the pixel level without the use of mask annotations. A total of seven phenotypic traits were extracted from images collected for a biparental population. Experimental results showed that optimal combinations of convolutional neural network and saliency methods achieved strong measurement correlations (r = 0.74 to 0.75) with human assessments at the image patch level, and the traits calculated by the saliency-based processing pipeline were highly correlated (r = 0.87 to 0.88) with reference PM infection ratings at the leaf image level. The high quantification accuracy of the saliency-based pipeline led to the increased explanation of phenotypic variance and reliable identification of quantitative trait loci. Therefore, the saliency-based processing pipeline can be used as an effective and efficient analysis tool for PM disease research and breeding programs in the future, especially agricultural and life science studies requiring microscopic image analysis.
“We Are Not Taking a Backseat”: Health Social Workers’ Perspectives on COVID-19 Response and Recovery
Authors: Ross Abigail M , Schneider Samantha , Boskey Elizabeth 
Published: August 2022

Abstract
The COVID-19 pandemic has wrought widespread devastation across the United States, exacerbating existing health inequities rooted in the social determinants of health. Social work is the key workforce tasked with providing social care in healthcare settings. In September 2019, the National Academies of Science, Engineering, and Medicine released a landmark Consensus Study Report, Integrating Social Care into the Delivery of Health Care: Moving Upstream to Improve the Nation’s Health. The report describes a framework for addressing key care needs that articulates five “As for action” (5As)—awareness, alignment, assistance, adjustment, and advocacy—that are critical to social care. Drawing from a series of focus groups conducted with 55 social workers employed in a large urban pediatric quaternary hospital, this study qualitatively examines the utility of this framework in characterizing social care activities during the pandemic response and recovery efforts. Findings suggest that the 5As framework is both applicable to pandemic social work practice and an accurate encapsulation of the core elements of hospital social work practice, even if social workers themselves may not necessarily be aware of that conceptualization. Future implications for social work practice in arenas of awareness, adjustment, assistance, alignment, and advocacy are also discussed.
Time-Dependent Moments From the Heat Equation and a Transport Equation
Authors: Curto Raúl E , di Dio Philipp J 
Published: August 2022

Abstract
We present a new connection between the classical theory of full and truncated moment problems and the theory of partial differential equations, as follows. For the classical heat equation $\partial _t u = {\nu } \Delta u$, with initial data $u_0 \in {\mathcal {S}}(\mathbb {R}^n)$, we first compute the moments $s_{\alpha }(t)$ of the unique solution $u \in {\mathcal {S}}(\mathbb {R}^n)$. These moments are polynomials in the time variable, of degree comparable to $\alpha $, and with coefficients satisfying a recursive relation. This allows us to define the polynomials for any sequence, and prove that they preserve some of the features of the heat kernel. In the case of moment sequences, the polynomials trace a curve (which we call the heat curve), which remains in the moment cone for positive time, but may wander outside the moment cone for negative time. This provides a description of the boundary points of the moment cone, which are also moment sequences. We also study how the determinacy of a moment sequence behaves along the heat curve. Next, we consider the transport equation $\partial _t u = ax \cdot \nabla u$ and conduct a similar analysis. Along the way we incorporate several illustrating examples. We show that while $\partial _t u = {\nu }\Delta u + ax\cdot \nabla u$ has no explicit solution, the time-dependent moments can be explicitly calculated.
Psychometric properties of the person-centred coordinated care experience questionnaire (P3CEQ) in a Norwegian radiotherapy setting
Authors: Sollid May ingvild volungholen , Slaaen Marit , Danielsen Signe , Kirkevold Øyvind 
Published: August 2022

Abstract

Background
The number of older adults with cancer is increasing. Radiotherapy is an important treatment modality for cancer and may cause side effects and distress. Patient-reported experience measures aim to measure patients’ experiences with health care. This can help healthcare services to improve in line with patients’ needs. To assess how Norwegian patients receiving radiotherapy experience their care, a valid and reliable tool is required. We selected the person-centred coordinated care experience questionnaire as a tool.

Objective
The aim of the study is to validate the Norwegian version of the person-centred coordinated care experience questionnaire in a radiotherapy setting.

Methods
A feasibility study of the person-centred coordinated care experience questionnaire and a cross-sectional study—testing psychometric properties of the questionnaire in a Norwegian radiotherapy setting—were conducted. Participants were recruited from two different hospitals in Norway. Patient characteristics and item scores are described using descriptive statistics. We performed an exploratory factor analysis and applied principal component analysis with a varimax rotation. Cronbach’s α was used to assess internal consistency.

Results
In total, 24 patients participated in the feasibility test, and 176 were included in the cross-sectional study where we explored the psychometric properties of the person-centred coordinated care experience questionnaire. Three factors were identified. Internal consistency was established for the 10-item scale, with Cronbach’s α = 0.698.

Conclusions
Conclusions must consider the Norwegian setting and healthcare context. We found that the Norwegian version of the person-centred coordinated care experience questionnaire is a relevant, valid and reliable tool to provide insight into different areas of patients’ experiences upon receiving radiotherapy. However, further testing on a larger sample is necessitated.
Sensitive and Rapid Detection of Escherichia coli O157:H7 From Beef Samples Based on Recombinase Aided Amplification Assisted CRISPR/Cas12a System
Authors: Fang Taisong , Shen Jinling , Xue Junxin , Jiang Yuan , Guo Dehua , Yang Jielin , Kong Xiangxiang , Xu Xuebin , Wang Xiang 
Published: August 2022

Abstract

Background
Escherichia coli O157:H7, being the cause of hemorrhagic colitis in humans, is recognized as one of the most dangerous and widespread foodborne pathogens. A highly specific, sensitive, and rapid E. coli O157:H7 detection method needs to be developed since the traditional detection methods are complex, costly, and time-consuming.

Objective
In this study, a recombinase aided amplification (RAA) assisted CRISPR/Cas12a (RAA-CRISPR/Cas12a) fluorescence platform for specific, sensitive, and rapid nucleic acid detection of E. coli O157:H7 was introduced.

Methods
First, the feasibility (components of CRISPR/Cas12a system) of the developed method was evaluated. Then a total of 34 bacterial strains were used for the specificity test, and gradient dilutions of extracted DNA and bacterial solutions of E. coli O157:H7 were prepared for the sensitivity test. Third, a real-time PCR assay for detection of the specific wzy gene of E. coli O157:H7 (FDA’s Bacteriological Analytical Manual) was used for sensitivity comparison. Finally, analysis of RAA-CRISPR/Cas12a detection in spiked and 93 real ground beef samples was carried out.

Results
The developed RAA-CRISPR/Cas12a method showed high specificity, and the detection could be completed within 30 min (after 4 h enrichment in spiked ground beef samples). The limit of detection (LOD) of bacterial concentrations and genomic DNA was 5.4 × 102 CFU/mL and 7.5 × 10−4 ng/μL, respectively, which exhibited higher sensitivity than the RAA-gel electrophoresis and RT-PCR methods. Furthermore, it was shown that E. coli O157:H7 in ground beef samples could be positively detected after 4 h enrichment when the initial bacterial inoculum was 9.0  CFU/25 g. The detection results of the RAA-CRISPR/Cas12a method were 100% consistent with those of the RT-PCR and traditional culture-based methods while screening the E. coli O157:H7 from 93 local collected ground beef samples.

Conclusions
The developed RAA-CRISPR/Cas12a method showed high specificity, high sensitivity, and rapid positive detection of E. coli O157:H7 from ground beef samples.

Highlights
The RAA-CRISPR/Cas12a system proposed in this study provided an alternative molecular tool for quick, specific, sensitive, and accurate detection of E. coli O157:H7 in foods.
The Law and Economics of Tying in Digital Platforms: Comparing Tencent and Android
Authors: Wu Qian , Philipsen Niels J 
Published: August 2022

Abstract
Tying has become a common practice in digital platforms. It may generate both pro-competitive effects and anti-competitive effects, which makes it difficult to distinguish between lawful and unlawful tying practices. The cases of Tencent and Android both involve tying conducts, but interestingly, the cases have different outcomes. This article explores reasons for these different case outcomes from a comparative law and economics perspective. By assessing the facts and legal rulings in Tencent and Android, we find that the different case outcomes result, on the one hand, from the different case facts, and on the other hand, from the different approaches used by the EU Commission and the Chinese Supreme People’s Court. The Court scores better in terms of ensuring legal certainty; nevertheless, it may face difficulties when it has to apply economic analysis. The Commission seemingly uses more economics, but the application is not full-fledged, as it disregards important case facts when assessing competition foreclosure, and employs asymmetric legal tests and evidence standards for anti/pro-competitive effects of tying. From a law and economics perspective, we provide suggestions for China and the EU, taking the recent Anti-Monopoly Guidelines on Platforms in China and the forthcoming Digital Markets Act in the EU into account.
Aboveground net primary productivity was not limited by phosphorus in a temperate typical steppe in Inner Mongolia
Authors: Guo Yumeng , Zhou Meng , Sheng Jun , Yuan Yujia , Yuan Guangyuan , Zhang Wen-Hao , Bai Wenming 
Published: August 2022

AbstractPhosphorus (P) is an essential element for plant growth, however, whether the aboveground net primary productivity (ANPP) of typical steppe was limited by P remains obscure. To detect the effects of P addition on primary productivity and aboveground biomass of different plant functional groups both under ambient and N addition conditions, ANPP and aboveground biomass of grasses and forbs were measured from 2016 to 2020 on a 16-year N and P addition experiment platform in a temperate typical steppe in Inner Mongolia. The soil available N and P concentrations were also determined to test the relationship between ANPP and the availability of soil nutrients. We found that P addition under ambient condition had no significant effect on ANPP and the aboveground biomass of grasses and forbs. However, under N addition, P addition significantly increased ANPP and the aboveground biomass of forbs. Furthermore, soil available N and P concentrations were increased significantly by N and P addition, respectively. Moreover, there was no significant correlation between ANPP and soil available P concentration, while ANPP was positively correlated with soil available N concentration. These results suggest that P is not the key factor limiting the primary productivity of the temperate typical steppe in Inner Mongolia. However, under N addition, P addition can promote ANPP and alter the community composition. These findings provide valuable information for the management of the temperate typical steppe.