bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brain white matter microstructure abnormalities in children with optimal outcome from autism: a four-year follow-up study
Authors: Zhang Manxue , Hu Xiao , Jiao Jian , Yuan Danfeng , Li Sixun , Luo Tingting , Wang Meiwen , Situ Mingjing , Sun Xueli , Huang Yi 
Published: November 2022

AbstractAutism spectrum disorder (ASD) is a lifelong neurodevelopmental disorder, with only a small proportion of people obtaining optimal outcomes. We do not know if children with ASD exhibit abnormalities in the white matter (WM) microstructure or if this pattern would predict ASD prognosis in a longitudinal study. 182 children with ASD were recruited for MRI and clinical assessment; 111 completed a four-year follow-up visit (30 with optimal outcomes, ASD−; 81 with persistent diagnosis, ASD+). Additionally, 72 typically developing controls (TDC) were recruited. The microstructural integrity of WM fiber tracts was revealed using tract-based spatial statistics (TBSS) and probabilistic tractography analyses. We examined the neuroimaging abnormality associated with ASD and its relationship to ASD with optimal outcome. The ASD+ and TDC groups were propensity score matched to the ASD− group in terms of age, gender, and IQ. TBSS indicated that children with ASD exhibited abnormalities in the superior longitudinal fasciculus (SLF), inferior longitudinal fasciculus (ILF), and extending to the anterior thalamic radiation (ATR) and cingulum; whereas the ASD+ group showed more severe abnormalities than the ASD- group. Probabilistic tractography analysis revealed that ASD+ group exhibited lower Fractional Anisotropy (FA) of the left superior thalamic radiation (STR L) than ASD− group, and that FA value of the STR L was a significant predictor of optimal outcome (EX(B), 6.25; 95% CI 2.50—15.63; p < 0.001). Children with ASD showed significant variations in SLF_L and STR_L, and STR_L was a predictor of ‘ASD with optimal outcome’. Our findings may aid in comprehension of the mechanisms of ‘ASD with optimal outcome’.
Fertilization treatments affect soil CO2 emission through regulating soil bacterial community composition in the semiarid Loess Plateau
Authors: Wang Jinbin , Xie Junhong , Li Lingling , Effah Zechariah , Xie Lihua , Luo Zhuzhu , Zhou Yongjie , Jiang Yuji 
Published: November 2022

AbstractA growing body of literature have emphasized the effects of fertilization regimes on soil respiration and microbial community in the semiarid region, however, fertilization treatment effects on the soil CO2emission, soil bacterial community, and their relationships from long-term experiments is lacking. In the present study, we investigated the effects of long-term fertilization regimes on soil bacterial community and thereafter on soil CO2emission. A 9-year field experiment was conducted with five treatments, including no fertilizer (NA) and four fertilization treatments (inorganic fertilizer (CF), inorganic plus organic fertilizer (SC), organic fertilizer (SM), and maize straw (MS)) with equal N input as N 200 kg hm–2. The results indicated that CO2emission was significantly increased under fertilization treatments compared to NA treatment. The bacterial abundance was higher under MS treatment than under NA treatment, while the Chao1 richness showed opposite trend. MS treatment significantly change soil bacterial community composition compared to NA treatment, the phyla (Alphaproteobacteria and Gammaproteobacteria) and potential keystone taxa (NitrosomonadaceaeandBeijerinckiaceae) were higher, while the Acidobacteriota was lower under MS treatment than under NA treatment. CO2emission was positively correlated with the abundance of Alphaproteobacteria, Gammaproteobacteria, and keystone taxa, negatively correlated with these of Acidobacteriota. Random forest modeling and structural equation modeling determined soil organic carbon, total nitrogen, and the composition and network module III of the bacterial community are the main factors contribute to CO2emission. In conclusion, our results suggest that the increased CO2emission was affected by the varied of soil bacterial community composition derived from fertilization treatments, which was related to Alphaproteobacteria, Gammaproteobacteria, Acidobacteriota, and potential keystone taxa (Nitrosomonadaceae and Beijerinckiaceae), and highlight that the ecological importance of the bacterial community in mediating carbon cycling in the semiarid Loess Plateau.
Multisensory-driven facilitation within the peripersonal space is modulated by the expectations about stimulus location on the body
Authors: Rossi Sebastiano Alice , Ronga Irene , Fossataro Carlotta , Galigani Mattia , Poles Karol , Garbarini Francesca 
Published: November 2022

AbstractCompelling evidence from human and non-human studies suggests that responses to multisensory events are fastened when stimuli occur within the space surrounding the bodily self (i.e., peripersonal space; PPS). However, some human studies did not find such effect. We propose that these dissonant voices might actually uncover a specific mechanism, modulating PPS boundaries according to sensory regularities. We exploited a visuo-tactile paradigm, wherein participants provided speeded responses to tactile stimuli and rated their perceived intensity while ignoring simultaneous visual stimuli, appearing near the stimulated hand (VTNear) or far from it (VTFar; near the non-stimulated hand). Tactile stimuli could be delivered only to one hand (unilateral task) or to both hands randomly (bilateral task). Results revealed that a space-dependent multisensory enhancement (i.e., faster responses and higher perceived intensity in VTNear than VTFar) was present when highly predictable tactile stimulation induced PPS to be circumscribed around the stimulated hand (unilateral task). Conversely, when stimulus location was unpredictable (bilateral task), participants showed a comparable multisensory enhancement in both bimodal conditions, suggesting a PPS widening to include both hands. We propose that the detection of environmental regularities actively shapes PPS boundaries, thus optimizing the detection and reaction to incoming sensory stimuli.
Effects of yam varieties on flour physicochemical characteristics and resultant instant fufu pasting and sensory attributes
Authors: Ufondu Helen E. , Maziya‐Dixon Busie , Okoyeuzu Chigozie F. , Okonkwo Thomas M. , Okpala Charles Odilichukwu R. 
Published: November 2022

AbstractChallenges and constraints deter the effective gathering of adequate information specific to the characteristics of yam (Dioscorea rotundata) landraces capable of producing a promising instant pounded fufu product. This current work, therefore, investigated the effects of yam varieties on flour physicochemical characteristics and resultant instant fufu pasting and sensory attributes. The D. rotundata varieties, obtained from the International Institute of Tropical Agriculture (IITA) (yam-breeding programme) experimental plots, included commercially available Fekatsa (control), alongside ten (N = 10) improved (varieties) namely: TDr 08/00068, TDr 10/00912, TDr 89/02665, TDr 95/01932, TDr 95/18544, TDr 97/00632, TDr 97/00917, TDr Agwekachi, TDr Ebute, and TDr Meccakusa). Compared to control, the improved yam varieties produced promising characteristic range values, for instance, amylose (15.77–33.89%), bulk density (0.77–0.86 g/cm3), setback (99.5–503.46 RVU), peak time (4.93–7.00 min) along with peak temperature (83.99 °C). From the correlation coefficient and principal component analysis, it was possible to deduce how flour physicochemical characteristics were associated with the resultant instant fufu pasting and sensory attributes. Considering the totality of results of this current study, both TDr 10/00912, and TDr Meccakusa yam varieties demonstrate a high potential on instant pounded flour towards producing an acceptable quality and promising fufu product.
IL-37 alleviates Coxsackievirus B3-induced viral myocarditis via inhibiting NLRP3 inflammasome-mediated pyroptosis
Authors: Sun Lin , Yuan Haitao , Zhao Gang 
Published: November 2022

AbstractOur study aims to verify the potential effects and underlying mechanisms of IL-37 in Coxsackievirus B3 (CVB3)-induced viral myocarditis (VMC). VMC model was established by intraperitoneal injection of CVB3 into 6-week-old male balb-c mice on day 0. Each mouse of the IL-37-control group and IL-37-VMC CVB3 groups was intraperitoneally injected with IL-37 on day 4 and day 7. The cardiac function was evaluated by transthoracic echocardiography including LVEF, LVFE, IVSs and IVSd. Myocardial injury was measured by Elisa for serum cTnI. The inflammation infiltration and fibrosis were evaluated by hematoxylin and eosin (HE) staining and Masson staining. The expression levels of NLRP3 inflammasome components in pyroptosis were determined by western blot, Elisa, and immunofluorescent analysis. We also detected the expression of IL-37-IL-1R8 in PBMCs by immunofluorescence after injection with CVB3 and IL-37. Compared with the VMC group, mice received CVB3 and IL-37 have improved cardiac function, reduced inflammation infiltration and fibrosis, and with lower expression of cTnI, IL-1β, IL-18 and NLRP3 inflammasome component. IL-37 weakened the upregulation of GSDMD and phosphorylation of NF-κB p65 induced by CVB3. Exogenous addition of IL-37 with CVB3 further increases the production of IL-37-IL-1R8 -IL-18RA complex in vitro. Our findings indicate that IL-37 alleviates CVB3-induced VMC, which may be produced by inhibiting NLRP3 inflammasome-mediated pyroptosis, NF-κB signaling pathway, and IL-37-IL-1R8 -IL-18RA complex.
Northeast Yucatan hurricane activity during the Maya Classic and Postclassic periods
Authors: Sullivan Richard M. , van Hengstum Peter J. , Donnelly Jeffrey P. , Tamalavage Anne E. , Winkler Tyler S. , Little Shawna N. , Mejia-Ortiz Luis , Reinhardt Eduard G. , Meacham Sam , Schumacher Courtney , Korty Robert 
Published: November 2022

AbstractThe collapse of the Maya civilization in the late 1st/early 2nd millennium CE has been attributed to multiple internal and external causes including overpopulation, increased warfare, and environmental deterioration. Yet the role hurricanes may have played in the fracturing of Maya socio-political networks, site abandonment, and cultural reconfiguration remains unexplored. Here we present a 2200 yearlong hurricane record developed from sediment recovered from a flooded cenote on the northeastern Yucatan peninsula. The sediment archive contains fine grain autogenic carbonate interspersed with anomalous deposits of coarse carbonate material that we interpret as evidence of local hurricane activity. This interpretation is supported by the correlation between the multi-decadal distribution of recent coarse beds and the temporal distribution of modern regional landfalling storms. In total, this record allows us to reconstruct the variable hurricane conditions impacting the northern lowland Maya during the Late Preclassic, Classic, and Postclassic Periods. Strikingly, persistent above-average hurricane frequency between ~ 700 and 1450 CE encompasses the Maya Terminal Classic Phase, the declines of Chichén Itza, Cobá, and subsequent rise and fall of the Mayapán Confederacy. This suggests that hurricanes may have posed an additional environmental stressor necessary of consideration when examining the Postclassic transformation of northern Maya polities.
Qualitative analysis and phase of chaos control of the predator-prey model with Holling type-III
Authors: AL-Kaff Mohammed O. , El-Metwally Hamdy A. , Elabbasy El-Metwally M. 
Published: November 2022

AbstractIn this study, we investigate the dynamics of a discrete-time with predator-prey system with a Holling-III type functional response model. The center manifold theorem and bifurcation theory are used to create existence conditions for flip bifurcations and Neimark-Sacker bifurcations. Bifurcation diagrams, maximum Lyapunov exponents, and phase portraits are examples of numerical simulations that not only show the soundness of theoretical analysis but also show complicated dynamical behaviors and biological processes. From the point of view of biology, this implies that the tiny integral step size can steady the system into locally stable coexistence. Yet, the large integral step size may lead to instability in the system, producing more intricate and richer dynamics. This also means that when the intrinsic death rate of the predator is high, this leads to a chaotic growth rate of the prey. The model has bifurcation features that are similar to those seen in logistic models. In addition, there is a bidirectional Neimark-Sacker bifurcation for both prey and predator, and therefore we obtain a direct correlation in symbiosis. This means that the higher the growth rate of the prey, the greater the growth rate of the predator. Therefore, the operation of predation has increased. The opposite is also true. Finally, the OGY approach is used to control chaos in the predator and prey model. which led to a new concept which we call bifurcation phase of control chaos.
Individualized anemia management enhanced by ferric pyrophosphate citrate protocol
Authors: Chait Yossi , Nathanson Brian H. , Germain Michael J. 
Published: November 2022

AbstractThe optimal use of erythropoiesis-stimulating agents (ESAs) and parenteral iron in managing anemia in end-stage renal disease (ESRD) remains controversial. One-size-fits-all rule-based algorithms dominate dosing protocols for ESA and parenteral iron. However, the Food & Drug Administration (FDA) guidelines for using ESAs in chronic kidney disease recommend individualized therapy for the patient. This prospective quality assurance project was at a single hemodialysis (HD) center comprising three 6-month phases (A, B, C) separated by 3-month washout periods. Standard bi-weekly ESA dose titration and intravenous (IV) iron sucrose protocols were used in baseline Phase A, and ferric pyrophosphate citrate (FPC) augmented iron in Phase B. In Phase C, an FPC protocol and weekly, individualized ESA management were used. We examined clinic-level mean differences in hemoglobin (Hb) and ESRD-related outcomes by phase with repeated ANOVA. To examine the Hb at the patient level, we used multi-level mixed-effect regression adjusting for phase, month, and other relevant confounders at each month over time to derive the mean marginal effects of phase. There were 54, 78, and 66 patients in phases A, B, and C, respectively, with raw mean Hb values of 9.9, 10.2, and 10.3 g/dL. The percentage of Hb values < 9 g/dL declined from 14.3% in Phase A to 7.6% in Phase C (p = 0.007). The multivariable mixed-effect regression results showed mean marginal Hb was higher by 0.3 mg/dL and 0.4 mg/dL in Phases B and C, respectively, compared to Phase A. We also observed reduced ferritin (p = 0.003) and transferrin saturation (TSAT) (p = 0.008) levels from Phase A to Phase C with the repeated ANOVA analysis. Ferric pyrophosphate citrate (FPC) appears to support more efficient ESA-stimulated erythropoiesis. Moreover, individualized ESA management combined with FPC (Phase C) was associated with further improvement in efficiency as we observed the fewest patients with Hb values < 9 g/dL concurrent with greater decreases in ferritin levels and reduced ESA doses. However, future prospective studies to confirm these findings on a larger, more diverse cohort of ESRD patients are warranted.
A portable analog front-end system for label-free sensing of proteins using nanowell array impedance sensors
Authors: Tayyab Muhammad , Xie Pengfei , Sami Muhammad Ahsan , Raji Hassan , Lin Zhongtian , Meng Zhuolun , Mahmoodi Seyed Reza , Javanmard Mehdi 
Published: November 2022

AbstractProteins are useful biomarkers for a wide range of applications such as cancer detection, discovery of vaccines, and determining exposure to viruses and pathogens. Here, we present a low-noise front-end analog circuit interface towards development of a portable readout system for the label-free sensing of proteins using Nanowell array impedance sensing with a form factor of approximately 35cm2. The electronic interface consists of a low-noise lock-in amplifier enabling reliable detection of changes in impedance as low as 0.1% and thus detection of proteins down to the picoMolar level. The sensitivity of our system is comparable to that of a commercial bench-top impedance spectroscope when using the same sensors. The aim of this work is to demonstrate the potential of using impedance sensing as a portable, low-cost, and reliable method of detecting proteins, thus inching us closer to a Point-of-Care (POC) personalized health monitoring system. We have demonstrated the utility of our system to detect antibodies at various concentrations and protein (45 pM IL-6) in PBS, however, our system has the capability to be used for assaying various biomarkers including proteins, cytokines, virus molecules and antibodies in a portable setting.
Insulin-like growth factor-1 levels are associated with high comorbidity of metabolic disorders in obese subjects; a Japanese single-center, retrospective-study
Authors: Kubo Haremaru , Sawada Shojiro , Satoh Michihiro , Asai Yoichiro , Kodama Shinjiro , Sato Toshihiro , Tomiyama Seitaro , Seike Junro , Takahashi Kei , Kaneko Keizo , Imai Junta , Katagiri Hideki 
Published: November 2022

AbstractInsulin like growth factor-1 (IGF-1) plays important roles in metabolic functions, especially in adulthood. Additionally, obese subjects are reportedly predisposed to having low absolute IGF-1 levels. However, the prevalence and clinical characteristics of obese subjects with low IGF-1 levels are unknown. We examined 64 obese subjects with a body mass index (BMI) ≥ 35 kg/m2, with no history of endocrinological disorders, receiving inpatient care. IGF-1 levels were interpreted based on the IGF-1 standard deviation score (SDS) clinically used and standardized by age and sex (low IGF-1 group; ≤ − 2.0 SDS and standard IGF-1 group; − 2.0 < and <  + 2.0 SDS). Notably, 26.6% of the subjects had low IGF-1. Body fat mass and percentage, but not BMI, were significantly higher in the low than in the standard IGF-1 group. Furthermore, natural log-transformed high-sensitivity C-reactive protein, and the frequencies of dyslipidemia and hyperuricemia were higher in the low IGF-1 group. Moreover, among the subjects without diabetes, fasting glucose levels were significantly higher in the low IGF-1 group. Stepwise variable selection procedure revealed body fat percentage to be a parameter most strongly associated with low IGF-1. Thus, low IGF-1 levels may be an important marker of adiposity-associated metabolic disorders in obese patients.
A rapid multiplex cell-free assay on biochip to evaluate functional aspects of double-strand break repair
Authors: Tatin Xavier , Muggiolu Giovanna , Libert Sarah , Béal David , Maillet Thierry , Breton Jean , Sauvaigo Sylvie 
Published: November 2022

AbstractThe repair of DNA double-strand breaks (DSBs) involves interdependent molecular pathways, of which the choice is crucial for a cell’s fate when facing a damage. Growing evidence points toward the fact that DSB repair capacities correlate with disease aggressiveness, treatment response and treatment-related toxicities in cancer. Scientific and medical communities need more easy-to-use and efficient tools to rapidly estimate DSB repair capacities from a tissue, enable routine-accessible treatment personalization, and hopefully, improve survival. Here, we propose a new functional biochip assay (NEXT-SPOT) that characterizes DSB repair-engaged cellular pathways and provides qualitative and quantitative information on the contribution of several pathways in less than 2 h, from 10 mg of cell lysates. We introduce the NEXT-SPOT technology, detail the molecular characterizations of different repair steps occurring on the biochip, and show examples of DSB repair profiling using three cancer cell lines treated or not with a DSB-inducer (doxorubicin) and/or a DNA repair inhibitor (RAD51 inhibitor; DNA-PK inhibitor; PARP inhibitor). Among others, we demonstrate that NEXT-SPOT can accurately detect decreased activities in strand invasion and end-joining mechanisms following DNA-PK or RAD51 inhibition in DNA-PK-proficient cell lines. This approach offers an all-in-one reliable strategy to consider DSB repair capacities as predictive biomarkers easily translatable to the clinic.
High daily energy expenditure of Tuvan nomadic pastoralists living in an extreme cold environment
Authors: Sellers Adam J. , Khovalyg Dolaana , Plasqui Guy , van Marken Lichtenbelt Wouter 
Published: November 2022

AbstractResearch investigating thermoregulatory energy costs in free-living humans is limited. We determined the total energy expenditure (TEE) of Tuvan pastoralists living in an extreme cold environment and explored the contribution of physical activity and cold-induced thermogenesis. Twelve semi-nomadic pastoralists (47 ± 8 years, 64 ± 8 kg) living under traditional circumstances, in Tuva, south-central Siberia, Russia, were observed during two consecutive 6-day periods in winter. TEE was measured via the doubly labelled water technique. Skin and ambient temperatures, and physical activity were continuously monitored. The outdoor temperature during the observation period was − 27.4 ± 5.4 °C. During the daytime, the participants were exposed to ambient temperatures below 0 °C for 297 ± 131 min/day. The Tuvan pastoralists were more physically active compared to western populations (609 ± 90 min/day of light, moderate, and vigorous physical activity). In addition, TEE was 13.49 ± 1.33 MJ/day (3224 ± 318 kcal/day), which was significantly larger by 17% and 31% than predicted by body mass, and fat-free mass, respectively. Our research suggests the daily cold exposure combined with high levels of physical activity contributed to the elevated TEE. Future research should reconsider the assumption that energy costs due to thermoregulation are negligible in free-living humans.
Movie editing influences spectators’ time perception
Authors: Kovarski Klara , Dos Reis Joanna , Chevais Claire , Hamel Anaïs , Makowski Dominique , Sperduti Marco 
Published: November 2022

AbstractFilmmakers use different techniques (e.g., camera movements, editing) to shape viewers' experience. In particular, editing can be used to handle the temporal unfolding of events represented in a movie. Nevertheless, little is known about how different editing types impact viewers’ time perception. In an exploratory on-line study (90 participants) and a pre-registered conceptual replication study (60 participants), we asked participants to judge (Study 1) or reproduce (Study 2) the duration of 45 excerpts of the movie “Le Ballon Rouge” containing either continuous editing, action discontinuity editing or no editing. Each excerpt was formatted in three durations (2000, 2500 or 3000 ms). In both studies, we reported that scenes containing continuous editing were perceived as longer than the other two scene types. Moreover, scenes containing action discontinuity editing were perceived as longer than scenes with no editing. This study contributes to the emerging field of psycho-cinematics which could ultimately develop the dialog between arts and science.