bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utilization of biosynthesized silica-supported iron oxide nanocomposites for the adsorptive removal of heavy metal ions from aqueous solutions
Authors: Garg Rishav , Garg Rajni , Khan Md. Amir , Bansal Manjeet , Garg Vinod 
Published: March 2022

Abstract
The present study deals with heavy metal ion removal from synthetic contaminated water using biosynthesized silica-supported iron oxide nanocomposites (nano-IOS). Agricultural and garden waste has been utilized to prepare nano-IOS through a green synthesis process. Nano-IOS was characterized by XRD, SEM, FTIR, and zeta potential analysis. The nanocomposites were used to adsorb heavy metal ions viz. Pb2+, Cd2+, Ni2+, Cu2+, and Zn2+ with optimization of reaction constraints including pH, the concentration of heavy metal ion, adsorbent dosage, and contact time in batch mode experiments. The optimized dose of nano-IOS was obtained as 0.75 g/L for adsorption of Pb2+, Cd2+, Ni2+, Cu2+, and Zn2+ (10.0 mg/L) with a contact duration of 70 min. at pH 5.0 for Pb2+, Cd2+, and Cu2+, and 6.0 for Ni2+ and Zn2+. The adsorption behavior of the nano adsorbent was well described by Langmuir adsorption isotherm and pseudo-second order kinetic models indicating chemisorption on the surface of nano-IOS. The adsorption was also found spontaneous and endothermic. Thus, the environmentally benign and bio-synthesized nano-IOS can be utilized as an effective nano-adsorbent for the rapid sequestration of heavy metal ions.
Establishment and Function of Chromatin Architecture at Eukaryotic Chromosome Replication Origins
Authors: Kurat Christoph , Chacin Erika , Reusswig Karl-Uwe , Furtmeier Jessica , Bansal Priyanka , Karl Leonhard , Pfander Boris , Korber Philipp 
Published: March 2022

Abstract
The Origin Recognition Complex (ORC) is essential for the initiation of eukaryotic chromosome replication as it loads the replicative helicase, the minichromosome maintenance (MCM) complex, at replication origins1.
These origins display a stereotypic chromatin structure. They are nucleosome depleted at ORC binding sites and flanked by regularly spaced nucleosome arrays2,3. Although discovered over a decade ago, we still do not know how this chromatin struture is established and if it matters for replication.
Here we show that ORC has an essential role in generating the origin chromatin structure. By genome-wide biochemical reconstitution we screened ~ 400 individual origins and 17 purified chromatin factors from budding yeast and found that ORC establishes both nucleosome depletion over origins as well as flanking nucleosome arrays by orchestrating the four chromatin remodelers INO80, ISW1a, ISW2 and Chd1. The importance of ORC’s chromatin organizing activity was underscored by ORC mutations that maintained classical MCM loader but lost array generation activity. These mutations were lethal in vivo.
Our results demonstrate that ORC, besides its canonical role as MCM loader, has a second essential function as master regulator of chromatin structure at replication origins, which is critical for chromosome replication.
Development of Atom Search Optimization Algorithm in the Optimal Operation of Single and Multi-Reservoir Systems (Case study: Dez Dam)
Authors: Moslemzadeh Mohsen , Karami Hojat , Farzin Saeed , Ahmadianfar Iman 
Published: March 2022

Abstract
The optimal operation of reservoirs is regarded as one of the most vital issues in water resources management, which also plays an important role in environmental processes and control of ecosystem pollution, especially in river water. The present study seeks to develop a new algorithm on single and multi-reservoir water resources systems, namely Atom Search Optimization (ASO). For this purpose, the algorithm is analyzed on mathematical benchmark functions. In the following, the algorithm's performance on the hypothetical systems is evaluated by performing sensitivity analysis on the effective coefficients of the algorithm, the number of particles, and the number of logical iterations to be implemented on four reservoir systems. The results of the best coefficients showed the proximity of 95.33% to the absolute solution of the four-reservoir system, i.e. 308.29, which implies the good and high accuracy of the algorithm. Then, to supply water downstream of Dez dam while addressing the monthly flow of sediment in the reservoir, which is a very important environmental phenomenon, the atom search algorithm and four other common optimization algorithms are applied, the results of which were prioritized using multi-criteria decision-making (MCDM) process by TOPSIS ranking technique. The results indicated the superiority of the Atom Search Algorithm (ASO), Salp Swarm Algorithm (SSA), Sine Cosine Algorithm (SCA), Particle Swarm Optimization (PSO) algorithm, and Genetic Algorithm (GA), respectively. Therefore, the use of the ASO algorithm in solving optimization problems, especially in the field of reservoir operations concerning environmental factors such as sedimentation is recommended.
Remodelling of the tumour-lymph node immune microenvironment promotes T- and NK-cell comobilization for tumour immunotherapy
Authors: Fu Shunli , Chang Lili , Liu Shujun , Gao Tong , Xiao Sang , Zhang Zipeng , Mu Weiwei , Liu Xiaoqing , Liang Shuang , Yang Han , Yang Huizhen , Ma Qingping , Liu Yongjun , Zhang Na 
Published: March 2022

Abstract
Reversing the suppressive tumour immune microenvironment has become a crucial strategy to enhance immunotherapy. Moreover, the immune lymph node (LN) microenvironment significantly influences the efficacy of immune cells. However, the critical role of the LN immune microenvironment on the regulation of antitumour effects is often ignored. Here, we developed the pH/MMP2/temperature triple-sensitive immunomodulatory nanoinducer NIL-IM-Lip, which can be precisely delivered to tumours and directed to the LNs. NIL-IM-Lip relieved the immunosuppressive effects of regulatory T cells (Treg) while coactivating cytotoxic T lymphocytes and natural killer cells both in tumours and LNs to reshape the tumour-lymph node immune microenvironment (TLIME) and enhance antitumour immunotherapy. Furthermore, the combined application of NIL-IM-Lip and the PD-1 mAb significantly enhanced the antitumour effectiveness of CTLs and NK cells. Notably, combination treatment with NIL-IM-Lip and the PD-1 mAb greatly suppressed tumour growth in both hot tumours (B16F10 model) and cold tumours (CT26 model) with a 20% complete response in the B16F10 model. Our work highlights the critical role of the TLIME in immunotherapy, displaying encouraging prospects when combined with PD-1 mAb treatment to strengthen antitumour immune effects, offering a new strategy for clinical use.
The differential impact of air pollutants on acute urticaria and chronic urticaria: a time series analysis
Authors: Han Xiuping , Li Jiawei , Song Guoqiang , Mu Zhenzhen , Lan Xiaoou , Yang Fan , Li Lin 
Published: March 2022

Abstract
Several studies have revealed links between short-term exposure to air pollution and the exacerbation of certain skin conditions. The present study sought to expand on these findings by exploring the potential relationship between exposure to air pollutants including particulate matter, sulfur dioxide, and ozone, and the incidence of acute and chronic urticaria in Shenyang, China, from 2016–2018. Exposure-response relationships between daily mean concentrations of these airborne pollutants and visits to outpatient dermatological clinics for acute urticaria and chronic urticaria were evaluated via a time series analysis approach using a generalized additive model. This analysis revealed that a 10 µg/m3 increase in daily mean O3_8h concentrations was associated with a 0.36% (95% CI, 0.31–0.41%), 0.35% (95% CI, 0.30–0.40%), and 0.34% (95% CI, 0.29–0.39%) increase in the number of outpatients for acute urticaria on the (lag0), lagging day 1 (lag1), and lagging day 2 (lag2), respectively. The increment in O3 levels remained statistically significant associated with outpatient for acute urticaria on cumulative lagging days. The increase in particulate matter levels had a similar cumulative effect on patients with chronic urticaria. In summary, these results indicated that short-term O3, PM2.5, and PM10 exposure can increase the risk of acute urticaria and chronic urticaria.
WITHDRAWN: Molecular Changes in Nucleus Accumbens of Accompanying with Companion Ameliorating CUMS-Induced Depressive-Like Behaviors
Authors: Li Bo , Zhang Hao , Cao Yi , Li Zhao , Xu Xinlei , Song Zhenhua , Wang Jin-hui 
Published: March 2022

Abstract
Social support are positively and causally related to mental health. Higher levels of perceived social support and group memberships are shown to be associated with lower depression symptomatology and recovery from depression, but the molecular biological mechanism behind it s remains largely unknown. Here, we report that accompanying with companion ameliorated CUMS-induced depression-like behaviors in mice. Accompanying with companion altered RNA expression profiles of nucleus accumbens (NAc) in CUMS-induced susceptible and resilience mice. 117 differentially expressed genes (DEGs) were found to be associated with depression-like behaviors, 17 DEGs associated with resilient behaviors, 43 DEGs associated with accompanying with companion. Importantly, 234 differentially expressed miRNAs that associated with accompanying with companion were obtained, and the miRNA-mRNA network associated with companion was established in NAc, based on the miRNA and mRNA profiles. Taken together, our findings revealed a potential new approach to improve depression-like behaviors, as well as many potential drug targets for the prevention or treatment of depression.
Expressional Variations of Kaiso: An Association with Pathological Characteristics and Field Cancerization of OSCC
Authors: Ahmed Shaheen , Khan Saeed , Qureshi Asif , Bukhari Uzma , Mughal Nouman , Anis Mehak 
Published: March 2022

Abstract
Background: In patients diagnosed with cancer it is considered that there is a field of tissue in the diagnosed organ containing cells with genetic aberration which pose a higher risk of transforming in to premalignant and malignant lesions. This field of altered cells is known as field of cancerization and is considered responsible for frequent recurrence of the cancer. Various molecules are being investigated for their significance in development of field cancerization and hence malignant diseases. Various members of POZ-ZF (poxvirus and zinc finger (POZ)-zinc finger) family of transcription factors are known to play a part in pathogenesis of neoplasm, one such protein of this family is Kaiso also known as ZBTB33 ( Zinc Finger and BTB Domain containing 33. Kaiso is a transcription factor known for having two modes of binding with DNA. The protein belongs to POZ-ZF family of transcription factors and may have functional tasks similar to its other siblings such as growth and development of vertebrates and pathogenesis of neoplastic diseases. Nevertheless, its role in field cancerization, pathogenesis and progression of oral cancer still needs exploration. Hence, this study was designed to explore the expressional differences between the mucosa of controls and those diagnosed with OSCC. Methods: Soft tissue samples were obtained from main tumor, tumor periphery and opposite buccal mucosa of 50 oral cancer patients, whereas normal mucosa was taken from 50 volunteers undergoing elective tooth removal. The acquired samples were then subjected to Immunohistochemical exploration for expression of Kaiso. The expression was measured using Image-J IHC profiler and summed as Optical density. The Optical density values were then subjected to statistical analysis. Results: Results revealed a significant differential expression of Kaiso between the mucosal tissues taken from oral cancer patients and controls (p-value: <0.0001) showing almost 50% down-regulation of Kaiso in all three tissue samples taken from oral cancer patients as compared to normal mucosa.Conclusion: Kaiso have significant difference of expression in the mucosa of oral cancer patients than the normal mucosa making it a probable contributor to disease pathogenesis and field cancerization.
Surgical management of flail chest with titanium plates: a French cohort series from a thoracic referral center
Authors: Féray Sarah , Blayau Clarisse , Masmoudi Hicham , Haddad Samuel , Quesnel Christophe , Assouad Jalal , Fartoukh Muriel 
Published: March 2022

Abstract
Background The development of titanium claw plates has made rib osteosynthesis easy to achieve and led to a renewed interest for this surgery. We report the management of patients referred to the intensive care unit (ICU) of a referral center for rib fracture fixation after chest trauma.MethodsA retrospective observational cohort study describing the patients’ characteristics and analyzing the determinants of postoperative complications. Results From November 2013 to December 2016, 42 patients were referred to our center: 12 patients (29%) suffered acute respiratory failure, 6 of whom were mechanically ventilated. The Thoracic Trauma Severity Score (TTSS) was 11.0 [9-12], with 7 [5-9] broken ribs and a flail chest in 92% of cases. A postoperative complication occurred in 18 patients (43%). Five patients developed ARDS (12%). Postoperative pneumonia occurred in 11 patients (26%). Two patients died in the ICU. In multivariable analysis, the TTSS (OR=1.89; CI95% 1.12-3.17; p=0.016) and the Simplified Acute Physiology Score II without age (OR=1.17; CI95% 1.02-1.34; p=0.024) were independently associated with the occurrence of a postoperative complication.ConclusionThe TTSS score appears more accurate than the Injury Severity Score for determining thoracic trauma severity. Rib fixation may be harmful, and short and long-term benefit should be assessed, particularly in non-mechanically ventilated patients.
Paracoccidioides sp infection impairs central nervous system cell junctional complexes
Authors: de Almeida Sergio Monteiro , Kulik Amanda , Malaquias Mineia Alessandra Scaranello , Nagashima Seigo , de Paula Caroline Busatta Vaz , Muro Marisol Dominguez , de Noronha Lucia 
Published: March 2022

Abstract
Paracoccidioidomycosis (PCM), a systemic mycosis caused by the fungus Paracoccidioides sp. is the most prevalent fungal infection among immunocompetent patients in Latin America. The estimated frequency of central nervous system (CNS) involvement among the human immunodeficiency virus (HIV)/PCM-positive population is 2.5%. We aimed to address the impact of neuroparacoccidioidomycosis (NPCM) and HIV/NPCM co-infection on the tight junctions (TJ) and adherens junction (AJ) proteins of the CNS. Four CNS formalin-fixed paraffin-embedded (FFPE) tissue specimens were studied: NPCM, NPCM/HIV co-infection, HIV-positive without opportunistic CNS infection, and normal brain autopsy (negative control). Immunohistochemistry was used to analyze the endothelial cells and astrocytes expressions of TJ markers: claudins (CLDN)-1, -3, -5 and occludin; AJ markers: β-catenin and E-cadherin; and pericyte marker: alpha-smooth muscle actin in formalin-fixed paraffin-embedded CNS tissue specimens were analyzed using the immunoperoxidase assay. CLDN-5 expression in the capillaries of the HIV/NPCM coinfected tissues (acute/subacute clinical form of PCM) was lower than that in the capillaries of the HIV or NPCM monoinfected (chronic clinical form of PCM) tissues. A marked decrease in CLDN-5 expression and a compensatory increase in CLDN-1 expression in the NPCM/HIV co-infection tissue samples was observed. The authors suggest that Paracoccidioides sp. crosses the blood–brain barrier through paracellular pathway, owing to the alteration in the CLDN expression, or inside the macrophages (Trojan horse).
Nurses’ satisfaction and experiences of redeployment during COVID-19- A cross-sectional survey
Authors: Chu Ginger , Connelly Kristy , Mexon Alexandra , Britton Ben , Tait Julie , Pitt Victoria , Inder Kerry 
Published: March 2022

Abstract
Background: Literature suggested that redeployment to an unfamiliar environment is a risk to nurses’ mental health. During the COVID-19 pandemic, many health professionals, particularly nurses, were required to be redeployed to different units to meet the changing demands of health service. This study aimed to evaluate nurses’ satisfaction and experiences of redeployment during COVID-19 and identify factors influencing redeployment satisfaction. Methods: A cross-sectional survey was developed to explore level of satisfaction and understand redeployment experiences. A total of 600 nurses from a tertiary teaching hospital in Australia were invited to complete an online survey from July to August 2020 with an estimated 25% of these experiencing redeployment as result of COVID-19 pandemic. Logistic regression was used to identify what impacts redeployment satisfaction. Redeployment experiences were analysed using iterative thematic approach.Results: A total of 98 surveys were analysed, with an estimated response rate of 65%. Forty-four percent of redeployed nurses were dissatisfied with redeployment and 53% reported a negative redeployment experience. Nurses who had a negative redeployment experience were five times more likely to be dissatisfied with redeployment (OR: 5.17, 95% CI: 2.12-12.5). These results were reflected in the qualitative findings where nurses reported that a positive redeployment experience such as “feeling welcome” and being given “fair patient allocation” eased their anxiety of being redeployed and created a positive perception on redeployment. Conclusion: Redeployment of healthcare workers during a pandemic is inevitable. This study highlighted that a large proportion of redeployed nurses feel dissatisfied with redeployment. Creating a positive redeployment experience is an important consideration to improve nurses’ satisfaction. In order to reduce the negative impact of dissatisfaction, more research needs to investigate how the negative redeployment experienced by nurses impacts on quality care and patient safety.
L1-ATP8B1 Exacerbates High-Level Reduction-Oxidation Homeostasis to Promote Carcinogenesis via Affecting PHB1 Sumoylation in LUSC
Authors: Zhang Xiao , Zhang Rui , Sun Zeguo , Liu Pengpeng , Chen Guidong , Ren Xiubao , Yu Wenwen , Zhang Weijia , Yu Jinpu 
Published: March 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Andrographolide Inhibits PMA-Induced EPCR Shedding through JNK and p38 MAPK
Authors: Changliang He , Zhenyu Wang , Xiaoxia Liang , Lizi Yin 
Published: March 2022

Abstract
Endothelial cell protein C (EPCR), one of the main members in the protein C (PC) pathway, plays an important role in the process of coagulation and inflammation. EPCR can shed from the cell membrane, which process is mediated by the tumor necrosis factor-α converting enzyme (TACE) and related to some diseases. Andrographolide is the major constituent of Andrographis paniculata, a kind of herbal medicine commonly used in clinical for its various pharmacological actions, especially anti-inflammation and anti-cancer. In this study, we investigated andrographolide protection for EPCR and its potential molecular mechanism. Phorbol-12-myristate-13-acetate (PMA)-stimulated human umbilical vein endothelial cells (HUVECs) were used to be the model of EPCR shedding and the andrographolide pretreatment was tested in this model. The results showed that andrographolide could reduce PMA-induced EPCR shedding and inhibit the TACE function. Additionally, we found andrographolide also suppressed the phosphorylation of c-Jun N-terminal kinase (JNK) and p38, but had no effect on extracellular regulated protein kinase (ERK) 1/2. Given these results, andrographolide can inhibit the shedding of EPCR by the suppression of TACE, which may take JNK and p38 as the molecular target. These findings indicated a substantial anti-EPCR shedding efficacy of andrographolide in vitro.
Are Urban Mangroves Emerging Hotspots of Non-Indigenous Species? A Study on the Dynamics of Macrobenthic Fouling Communities in Fringing Red Mangrove Prop Roots
Authors: Mosquera Enis , Blanco-Libreros Juan , Riascos José M. 
Published: March 2022

Abstract
Urbanization represent a radical transformation of natural habitats that alters all the biotic and abiotic properties governing ecosystems. Urban expansion often results in oversimplified communities, where most specialists decline or disappear and a few generalist or exotic species become dominant. The consequences of urban expansion in mangrove forests are understudied, although these systems have been altered by humans through centuries and the growth of human population in tropical coasts is expected to be faster than in higher latitudes. To assess the importance of indigenous and non-indigenous species in driving temporal and spatial changes in community structure of red-mangrove prop-root macrobenthic communities, we studied heavily altered mangrove forests from two bays from the Caribbean coast of Colombia in 2005 and 2021. In all places/periods, the community richness was low, a few taxa were dominant (11 taxa, out of 40, comprised ~ 90% of the total abundance) and the majority of all taxa (65%) were non-indigenous species whose presence is related with known stressors in urbanized systems. Hence, we suggest that urban mangrove forests are emerging hotspots for non-indigenous biota. Community structure did not change within or between bays, there was a clear, significant turnover of core species between 2005 and 2021, with non-indigenous species playing a prominent role in this variability. This was puzzling –ecological theory asserts that the abundance of a species is related to their permanence: core species are relatively stable through time while rare species appear or disappear– but this may not apply for communities dominated by non-indigenous biota.