bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating the effects of an intervention to improve the health environment for mothers and children in health centres (BECEYA) in Mali: a qualitative study
Authors: Ngangue Patrice , Robert Katherine , Ly Birama Apho , Traoré Fatoumata , Vezina Maude 
Published: April 2022

AbstractBackgroundAn intervention aiming to improve maternal and children environment in healthcare facilities (BECEYA) was launched in three regions of Mali. This study aimed to explore the perceptions and experiences of patients and their companions, community actors and healthcare facilities staff on the effects of the BECEYA intervention in two regions of Mali.MethodsWe conducted a qualitative study using an empirical phenomenological approach. Through purposive sampling, women who attended antenatal care in the selected healthcare centres, companions, and health facility staff members were recruited. Data were collected during January and February 2020 through semi-structured individual interviews and focus groups. According to Braun & Clarke approach, audio recordings were transcribed verbatim, and a thematic analysis was conducted in five main steps.ResultsWe recruited 26 participants in individual interviews and 20 participants in focus groups. Donabedian conceptual framework of quality of care was used to present the perceived changes following the implementation of the BECEYA project. The themes that emerged from data analysis are perceived changes in terms of infrastructure (perceived changes in the characteristics of the healthcare facilities setting, including the infrastructure introduced by the BECEYA project), process (changes in the delivery and use of care introduced or resulting from BECEYA activities), and outcome (the direct and indirect effects of these changes on the health status of patients and the population).ConclusionThe study identified the positive effects of women users of the services, their companions and health centre staff following the implementation of the project. Therefore, this study contributes to the advancement of knowledge to show the link between improving the health environment of health centres in developing countries, a major aspect of the quality of care, and maternal and child health care.
A subtype of melanopsin ganglion cells encodes ground luminance
Authors: Berry Michael H. , Moldavan Michael , Garrett Tavita , Meadows Marc , Cravetchi Olga , White Elizabeth , von Gersdorff Henrique , Wright Kevin M , Allen Charles , Sivyer Benjamin 
Published: April 2022

AbstractVisual input to the hypothalamus from intrinsically photosensitive retinal ganglion cells (ipRGCs) influences several functions including circadian entrainment, body temperature, and sleep. ipRGCs also project to nuclei such as the supraoptic nucleus (SON), which is involved in systemic fluid homeostasis, maternal behavior, and appetite. However, little is known about the SON-projecting ipRGCs or their relationship to well-characterized ipRGC subtypes. Using a GlyT2Cre mouse line, we identify a subtype of ipRGCs restricted to the dorsal retina that selectively project to the SON. These ipRGCs form a non-overlapping tiled mosaic that is limited to a dorsal region of the retina, forming a substrate for encoding ground luminance. Optogenetic activation of their axons demonstrates they release the neurotransmitter glutamate and that the SON is retinorecipient, receiving synaptic input from dorsal ipRGCs. Our results challenge the idea that ipRGC dendrites overlap to optimize photon capture and suggests non-image forming vision operates to sample local regions of the visual field.
Social network analysis reveals context-dependent kin relationships in wild sulphur-crested cockatoos,Cacatua galerita
Authors: Penndorf J. , Ewart K. M. , Klump B. C. , Martin J. M. , Aplin L. M. 
Published: April 2022

AbstractA preference to associate with kin facilitates inclusive fitness benefits, and increased tolerance or cooperation between kin may be an added benefit of group living. Many species exhibit preferred associations with kin, however it is often hard to disentangle active preferences from passive overlap, for example caused by limited dispersal or inheritance of social position.Many parrots exhibit social systems consisting of pair-bonded individuals foraging in variably sized fission-fusion flocks within larger communal roosts of hundreds of individuals. Previous work has shown that, despite these fission-fusion dynamics, individuals can exhibit long-term preferred foraging associations outside their pair-bonds. Yet the underlying drivers of these social preferences remains largely unknown.In this study, we use a network approach to examine the influence of kinship on social associations and interactions in wild, communally roosting sulphur-crested cockatoos,Cacatua galerita. We recorded roost co-membership, social associations and interactions in 561 individually marked birds across three neighbouring roosts. We then collected genetic samples from 205 cockatoos, and conducted a relationship analysis to construct a kinship network. Finally, we tested correlations between kinship and four social networks: association, affiliative, low-intensity aggression, and high-intensity aggression.Our result showed that while roosting groups were clearly defined, they showed little genetic differentiation or kin structuring. Between roost movement was high, with juveniles, especially females, repeatedly moving between roosts. Both within roosting communities, and when visiting different roosts, individuals preferentially associated with kin. Supporting this, individuals were also more likely to allopreen kin. However, contrary to expectation, individuals preferred to direct aggression towards kin, with this effect only observed when individuals shared roost membership.By measuring social networks within and between large roosting groups, we could remove potential effects of passive spatial overlap on kin structuring. Our study reveals that sulphur-crested cockatoos actively prefer to associate with kin, both within and between roosting groups. By examining this across different interaction types, we further demonstrate that sulphur-crested cockatoos exhibit behavioural and context-dependent interaction rules towards kin. Our results help reveal the drivers of social association in this species, while adding to the evidence for social complexity in parrots.
Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cord
Authors: Song Janet H.T. , Ruven Carolin , Patel Payal , Ding Frances , Macklis Jeffrey D. , Sahni Vibhu 
Published: April 2022

AbstractCorticospinal neurons (CSN) are centrally required for skilled voluntary movement, which necessitates that they establish precise subcerebral connectivity with the brainstem and spinal cord. However, molecular controls regulating specificity of this projection targeting remain largely unknown. We previously identified that developing CSN subpopulations exhibit striking axon targeting specificity in the spinal white matter. These CSN subpopulations with segmentally distinct spinal projections are also molecularly distinct; a subset of differentially expressed genes between these distinct CSN subpopulations function as molecular controls regulating differential axon projection targeting. Rostrolateral CSN extend axons exclusively to bulbar-cervical segments (CSNBC-lat), while caudomedial CSN (CSNmedial) are more heterogeneous, with distinct, intermingled subpopulations extending axons to either bulbar-cervical or thoraco-lumbar segments. Here, we report that Cerebellin 1 (Cbln1) is expressed specifically by CSN in medial, but not lateral, sensorimotor cortex. Cbln1 shows highly dynamic temporal expression, with Cbln1 levels in CSN highest during the period of peak axon extension toward thoraco-lumbar segments. Using gain-of-function experiments, we identify that Cbln1 is sufficient to direct thoraco-lumbar axon extension by CSN. Mis-expression of Cbln1 in CSNBC-lat either by in utero electroporation, or in postmitotic CSNBC-lat by AAV-mediated gene delivery, re-directs these axons past their normal bulbar-cervical targets toward thoracic segments. Further, Cbln1 overexpression in postmitotic CSNmedial increases the number of CSNmedial axons that extend past cervical segments into the thoracic cord. Collectively, these results identify that Cbln1 functions as a potent molecular control over thoraco-lumbar CSN axon extension, part of an integrated network of controls over segmentally-specific CSN axon projection targeting.Significance StatementCorticospinal neurons (CSN) exhibit remarkable diversity and precision of axonal projections to targets in the brainstem and distinct spinal segments; the molecular basis for this targeting diversity is largely unknown. CSN subpopulations projecting to distinct targets are also molecularly distinguishable. Distinct subpopulations degenerate in specific motor neuron diseases, further suggesting that intrinsic molecular differences might underlie differential vulnerability to disease. Here, we identify a novel molecular control, Cbln1, expressed by CSN extending axons to thoraco-lumbar spinal segments. Cbln1 is sufficient, but not required, for CSN axon extension toward distal spinal segments, and Cbln1 expression is controlled by recently identified, CSN-intrinsic regulators of axon extension. Our results identify that Cbln1, together with other regulators, coordinates segmentally precise CSN axon targeting.
Ectopic insert-dependent neuronal expression of GFAP promoter-driven AAV constructs in adult mouse retina
Authors: Le Nguyet , Appel Haley , Pannullo Nicole , Hoang Thanh , Blackshaw Seth 
Published: April 2022

AbstractDirect reprogramming of retinal Müller glia is a promising avenue for replacing photoreceptors and retinal ganglion cells lost to retinal dystrophies. However, questions have recently been raised about the accuracy of studies claiming efficient glia-to-neuron reprogramming in retina that were conducted using GFAP mini promoter-driven adeno-associated virus (AAV) vectors. In this study, we have addressed these questions using GFAP mini promoter-driven AAV constructs to simultaneously overexpress the mCherry reporter and candidate transcription factors predicted to induce glia-to-neuron conversion, in combination with prospective genetic labeling of retinal Müller glia using inducible Cre-dependent GFP reporters. We find that, while control GFAP-mCherry constructs express faithfully in Müller glia, 5 out of 7 transcription factor overexpression constructs tested are predominantly expressed in amacrine and retinal ganglion cells. However, genetic cell lineage analysis shows no evidence for glia-to-neuron conversion. These findings demonstrate strong insert-dependent effects on AAV-based GFAP mini promoter specificity that preclude its use in inferring cell lineage relationships when studying glia-to-neuron conversion in retina.
Neural representation of intraoral olfactory and gustatory signals by the mediodorsal thalamus in alert rats
Authors: Fredericksen Kelly E. , Samuelsen Chad L. 
Published: April 2022

AbstractThe mediodorsal thalamus is a higher-order thalamic nucleus involved in a variety of cognitive behaviors, including olfactory attention, odor discrimination, and the hedonic perception of flavors. Although it forms connections with principal regions of the olfactory and gustatory networks, its role in processing olfactory and gustatory signals originating from the mouth remains unclear. Here, we recorded single-unit activity in the mediodorsal thalamus of behaving rats during the intraoral delivery of individual odors, individual tastes, and odor-taste mixtures. Our results are the first to demonstrate that neurons in the mediodorsal thalamus dynamically encode chemosensory signals originating from the mouth. This chemoselective population is broadly tuned, responds with excitation and suppression, and represents odor-taste mixtures differently than their odor and taste components. Furthermore, a subset of chemoselective neurons encoded taste palatability. Our results further demonstrate the multidimensionality of the mediodorsal thalamus and provides additional evidence of its involvement in processing chemosensory information important for consummatory behaviors.SIGNIFICANCE STATEMENTThe perception of food relies upon the concurrent processing of olfactory and gustatory signals originating from the mouth. The mediodorsal thalamus is a higher-order thalamic nucleus involved in a variety of chemosensory-dependent behaviors and connects the olfactory and gustatory cortices with prefrontal cortex. However, it is unknown how neurons in the mediodorsal thalamus process intraoral chemosensory signals. Using tetrode recordings in alert rats, our results are the first to show that neurons in the mediodorsal thalamus dynamically represent olfactory and gustatory signals from the mouth. Our findings suggest that the mediodorsal thalamus is a key node between sensory and higher-order cortical areas for processing chemosensory information underlying consummatory behavior.
Platelet response to influenza vaccination reflects effects of aging
Authors: Konstorum A. , Mohanty S. , Zhao Y. , Melillo A. , Vander Wyk B. , Nelson A. , Tsang S. , Blevins T.P. , Belshe R.B. , Chawla D.G. , Rondina M.T. , Montgomery R.R. , Allore H.G. , Kleinstein S.H. , Shaw A.C. 
Published: April 2022

AbstractPlatelets are uniquely positioned as mediators of not only hemostasis but also innate immunity, but how age and alterations in functional status such as frailty influence platelet function during an immune response remains unclear. We assessed the platelet transcriptome in younger (age 21-35) and older (age ≥ 65) adults (including frail and non-frail individuals) following influenza vaccination. Prior to vaccination, we identified an age- and frailty-associated increase in expression of platelet activation and mitochondrial RNAs and decrease in RNAs encoding proteins mediating translation. Using tensor decomposition analysis, we also elucidated dynamic post-vaccination platelet activation and translation signatures associated with age and frailty. At the protein level, enhanced platelet activation was found in non-frail older adults, compared to young individuals both prior to and post-vaccine; but frail adults showed decreased platelet activation compared to non-frail that could reflect the influence of decreased translation RNA expression. Our results reveal an age-dependent alteration in platelet function prior to and post-vaccination that may contribute to age-associated chronic inflammation.
Computational Analysis of Effects of Clot Length on Acute Ischemic Stroke Recanalization under Different Cyclic Aspiration Loading Conditions
Authors: Patki Priyanka , Simon Scott , Manning Keefe B. , Costanzo Francesco 
Published: April 2022

AbstractAcute ischemic stroke, the second leading cause of death worldwide, results from occlusion of a cerebral artery by a blood clot. Application of cyclic aspiration using an aspiration catheter is a current therapy for the removal of lodged clots. In this study, we perform finite element simulations to analyze deformation of long clots, having length to radius ratio of 2 to 10, which corresponds to clot-length of 2.85–14.25 mm, under peak-to-peak cyclic aspiration pressures of 10 to 50mmmHg, and frequencies of 0.5, 1 and 2 Hz. Our computational system comprises of a nonlinear viscoelastic solid clot, a hyperelastic artery, and a nonlinear viscoelastic cohesive zone, the latter modeling the clot–artery interface. We observe that clots having length-to-radius ratio approximately greater than two separate from the inner arterial surface somewhere between the axial and distal ends, irrespective of the cyclic aspiration loading conditions. The stress distribution within the clot shows large tensile stresses in the clot interior, indicating the possibility of simultaneous fragmentation of the clot. Thus, this study shows us the various failure mechanisms simultaneously present in the clot during cyclic aspiration. Similarly, the stress distribution within the artery implies a possibility of endothelial damage to the arterial wall near the end where the aspiration pressure is applied. This framework provides a foundation for further investigation to clot fracture and adhesion characterization.
A tonically active master neuron modulates mutually exclusive motor states at two timescales
Authors: Meng Jun , Ahamed Tosif , Yu Bin , Hung Wesley , Mouridi Sonia EI , Wang Zezhen , Zhang Yongning , Wen Quan , Boulin Thomas , Gao Shangbang , Zhen Mei 
Published: April 2022

AbstractContinuity of behaviors requires animals to make smooth transitions between successive and mutually exclusive behavioral states. Neural principles that govern these transitions are not well understood.C. elegansspontaneously switch between two opposite motor states, forward and backward movement, a phenomenon long thought to reflect the reciprocal inhibition between two interneurons that separately gate the forward and backward motor circuits, AVB and AVA. Combining experimental data and mathematical modeling, we report that spontaneous forward and backward locomotion and their corresponding motor circuits are not separately controlled. AVA and AVB are neither functionally equivalent nor strictly reciprocally inhibitory. Instead, while AVA phasically inhibits the forward promoting interneuron AVB at a fast timescale, it maintains a tonic, extrasynaptic excitation on AVB over the longer timescale. AVA’s depolarized spontaneous membrane potential is necessary for this tonic excitation. We propose a new, master neuron model for locomotion. AVA, with tonic and phasic activity of opposite polarities on different time scales, acts as a master neuron to break the symmetry between the underlying forward and backward motor circuits. This offers a parsimonious solution for sustained locomotion consisted of mutually exclusive motor states.TeaserA tonically activeC. eleganspremotor interneuron functions as the master neuron that underlies continuous modulation of forward and backward movement to ensure smooth transitions between the two opposing motor states.
Mechanisms of human dynamic object recognition revealed by sequential deep neural networks
Authors: Sörensen Lynn K. A. , Bohté Sander M. , de Jong Dorina , Slagter Heleen A. , Scholte H. Steven 
Published: April 2022

AbstractHumans can rapidly recognize objects in a dynamically changing world. This ability is showcased by the fact that observers succeed at recognizing objects in rapidly changing image sequences, at up to 13 ms/image. To date, the mechanisms that govern dynamic object recognition remain poorly understood. Here, we developed deep learning models for dynamic recognition and compared different computational mechanisms, contrasting feedforward and recurrent, single-image and sequential processing as well as different forms of adaptation. We found that only models that integrate images sequentially via lateral recurrence mirrored human performance (N=36) and were predictive of trial-by-trial responses across image durations (13-80 ms/image) while also displaying a temporal correspondence. Augmenting this model with adaptation markedly improved dynamic recognition and accelerated its representational dynamics, thereby predicting human trial-by-trial responses using fewer processing resources. These findings provide new insights into the mechanisms rendering object recognition so fast and effective in a dynamic visual world.
Multimodal deep neural decoding of visual object representation in humans
Authors: Watanabe Noriya , Miyoshi Kosuke , Jimura Koji , Shimane Daisuke , Keerativittayayut Ruedeerat , Nakahara Kiyoshi , Takeda Masaki 
Published: April 2022

AbstractPerception and categorization of objects in a visual scene are essential to grasp the surrounding situation. However, it is unclear how neural activities in spatially distributed brain regions, especially in terms of temporal dynamics, represent visual objects. To address this issue, we explored the spatial and temporal organization of visual object representations using concurrent functional magnetic resonance imaging (fMRI) and electroencephalography (EEG), combined with neural decoding using deep neural networks (DNNs). Visualization of the fMRI DNN revealed that visual categorization (faces or non-face objects) occurred in brain-wide cortical regions, including the ventral temporal cortex. Interestingly, the EEG DNN valued the earlier phase of neural responses for categorization and the later phase of neural responses for sub-categorization. Combination of the two DNNs improved the classification performance for both categorization and sub-categorization. These deep learning-based results demonstrate a categorization principle in which visual objects are represented in a spatially organized and coarse-to-fine manner.
Single nuclei RNAseq stratifies multiple sclerosis patients into distinct white matter glial responses
Authors: Macnair Will , Calini Daniela , Agirre Eneritz , Bryois Julien , Jäkel Sarah , Kukanja Petra , Stokar Nadine , Ott Virginie , Foo Lynette C. , Collin Ludovic , Schippling Sven , Urich Eduard , Nutma Erik , Marzin Manuel , Amor Sandra , Magliozzi Roberta , Heidari Elyas , Robinson Mark , ffrench-Constant Charles , Castelo-Branco Gonçalo , Williams Anna , Malhotra Dheeraj 
Published: April 2022

AbstractThe lack of understanding of the cellular and molecular basis of clinical and genetic heterogeneity in progressive multiple sclerosis (MS) has hindered the search for new effective therapies. Here, to address this gap, we analysed 632,000 single nuclei RNAseq profiles of 156 brain tissue samples, comprising white matter (WM) lesions, normal appearing WM, grey matter (GM) lesions and normal appearing GM from 54 MS patients and 26 controls. We observed the expected changes in overall neuronal and glial numbers previously described within the classical lesion subtypes. We found highly cell type-specific gene expression changes in MS tissue, with distinct differences between GM and WM areas, confirming different pathologies. However, surprisingly, we did not observe distinct gene expression signatures for the classical different WM lesion types, rather a continuum of change. This indicates that classical lesion characterization better reflects changes in cell abundance than changes in cell type gene expression, and indicates a global disease effect. Furthermore, the major biological determinants of variability in gene expression in MS WM samples relate to individual patient effects, rather than to lesion types or other metadata. We identify four subgroups of MS patients with distinct WM glial gene expression signatures and patterns of oligodendrocyte stress and/or maturation, suggestive of engagement of different pathological processes, with an additional more variable regenerative astrocyte signature. The discovery of these patterns, which were also found in an independent MS patient cohort, provides a framework to use molecular biomarkers to stratify patients for optimal therapeutic approaches for progressive MS, significantly advances our mechanistic understanding of progressive MS, and highlights the need for precision-medicine approaches to address heterogeneity among MS patients.