bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multicenter validation of PIM3 and PIM2 in Brazilian pediatric intensive care units
Authors: Genu Daniel Hilário Santos , Lima-Setta Fernanda , Colleti José , de Souza Daniela Carla , Gama Sérgio D’Abreu , Massaud-Ribeiro Letícia , Pistelli Ivan Pollastrini , Proença Filho José Oliva , Bernardi Thaís de Mello Cesar , de Castilho Taísa Roberta Ramos Nantes , Clemente Manuela Guimarães , Borsetto Cibele Cristina Manzoni Ribeiro , de Oliveira Luiz Aurelio , Alves Thallys Ramalho Suzart , Pedroso Diogo Botelho , La Torre Fabíola Peixoto Ferreira , Borges Lunna Perdigão , Santos Guilherme , Mello e Silva Juliana Freitas de , de Magalhães-Barbosa Maria Clara , da Cunha Antonio José Ledo Alves , Soares Marcio , Prata-Barbosa Arnaldo ,   The Brazilian Research Network in Pediatric Intensive Care (BRnet-PIC)
Published: December 2022

ObjectiveTo validate the PIM3 score in Brazilian PICUs and compare its performance with the PIM2.MethodsObservational, retrospective, multicenter study, including patients younger than 16 years old admitted consecutively from October 2013 to September 2019. We assessed the Standardized Mortality Ratio (SMR), the discrimination capability (using the area under the receiver operating characteristic curve – AUROC), and the calibration. To assess the calibration, we used the calibration belt, which is a curve that represents the correlation of predicted and observed values and their 95% Confidence Interval (CI) through all the risk ranges. We also analyzed the performance of both scores in three periods: 2013–2015, 2015–2017, and 2017–2019.Results41,541 patients from 22 PICUs were included. Most patients aged less than 24 months (58.4%) and were admitted for medical conditions (88.6%) (respiratory conditions = 53.8%). Invasive mechanical ventilation was used in 5.8%. The median PICU length of stay was three days (IQR, 2–5), and the observed mortality was 1.8% (763 deaths). The predicted mortality by PIM3 was 1.8% (SMR 1.00; 95% CI 0.94–1.08) and by PIM2 was 2.1% (SMR 0.90; 95% CI 0.83–0.96). Both scores had good discrimination (PIM3 AUROC = 0.88 and PIM2 AUROC = 0.89). In calibration analysis, both scores overestimated mortality in the 0%–3% risk range, PIM3 tended to underestimate mortality in medium-risk patients (9%–46% risk range), and PIM2 also overestimated mortality in high-risk patients (70%–100% mortality risk).ConclusionsBoth scores had a good discrimination ability but poor calibration in different ranges, which deteriorated over time in the population studied.
Genetic dissection of quantitative trait loci for flag leaf size in bread wheat (Triticum aestivum L.)
Authors: Chen Liangen , Xu Zhibin , Fan Xiaoli , Zhou Qiang , Yu Qin , Liu Xiaofeng , Liao Simin , Jiang Cheng , Lin Dian , Ma Fang , Feng Bo , Wang Tao 
Published: December 2022

Flag leaf size is a crucial trait influencing plant architecture and yield potential in wheat. A recombinant inbred line (RIL) population derived from the cross of W7268 and Chuanyu 12 was employed to identify quantitative trait loci (QTL) controlling flag leaf length (FLL), flag leaf width (FLW), and flag leaf area (FLA) in six environments and the best linear unbiased estimator (BLUE) datasets. Using a 55 K SNP-based genetic map, six major and stable QTL were detected with 6.33–53.12% of explained phenotypic variation. Except for QFlw.cib-4B.3, the other five major QTL were co-located within two intervals on chromosomes 2B and 2D, namely QFll/Fla.cib-2B and QFll/Flw/Fla.cib-2D, respectively. Their interactions and effects on the corresponding traits and yield-related traits were also assessed based on flanking markers. QFll/Fla.cib-2B showed pleiotropic effects on spikelet number per spike (SNS). QFlw.cib-4B.3 and QFll/Flw/Fla.cib-2D had effects on grain number per spike (GNS) and thousand-grain weight (TGW). Comparison analysis suggested that QFll/Fla.cib-2B was likely a new locus. Two candidate genes, TraesCS2B03G0222800 and TraesCS2B03G0230000, associated with leaf development within the interval of QFll/Fla.cib-2B were identified based on expression-pattern analysis, gene annotation, ortholog analysis, and sequence variation. The major QTL and markers reported here provide valuable information for understanding the genetic mechanism underlying flag leaf size as well as breeding utilization in wheat.
Use of stability statistics in the selection of Clausena heptaphylla (Roxb.) Wight & Arn for novel anethole rich strain (Jor Lab CH-2)
Authors: Lal Mohan , Munda Sunita , Gogoi Anindita , Begum Twahira , Baruah Joyashree , Chanda Sanjoy K. , Lekhak Himangshu 
Published: December 2022

IntroductionAnethole is an industrially important compound which is extensively used in pharmaceuticals, cosmetics, perfumery, food and confectioneries. Anethole is primarily obtained from fennel, anise, and star anise which is highly expensive. Therefore, a study was performed to identify a cost-effective and natural anethole rich strain of Clausena heptaphylla through selection and confirmed through multilocation trial.MethodsThe study was conducted using 23 accessions collected from North eastern region of India from 2014-2018 (initial evaluation trial) and 2018-2022 (multilocation trial). The initial trial was conducted in the experimental farm of CSIR-NEIST, Jorhat, Assam using Complete Randomized Block Design with three replications. Five agronomical traits (plant height, leaf length, leaf width, number of stem branching, herbage yield per plant per cutting) along with essential oil yield and anethole content were evaluated which led to the identification of anethole rich strain of C. heptaphylla. This identified strain was further evaluated along with the two check genotypes for stability based on three yield parameters viz. herbage yield, essential oil yield and anethole content at four multi-locations (Imphal, Jorhat, Runne and Madang) for four years using the same experimental design.Results and discussionThe identified superior line (Jor Lab CH-2) showed consistent performance for the studied yield parameters across all the environments maintaining its superiority. The identified strain exhibited average herbage yield of 1.2 Kg/plant/cutting and essential oil yield of 1.22%. The GC-MS analysis of the essential oil depicted trans anethole as the major constituent (93.25%) followed by estragole (4.85%) while benzene, 1,2-dimethoxy-4-(1-propenyl Isoeugenol methyl ether and cis-anethole were the trace components. This is the first novel report of anethole rich variant of C. heptaphylla which has undergone multilocational trial over the years. Jor Lab CH-2 strain will open a new scope for the industries to isolate anethole from a different source in a cost-effective approach.
Genome-wide identification and comprehensive analysis of tubby-like protein gene family in multiple crops
Authors: Zeng Yafei , Wen Jianyu , Fu Jinmei , Geng Han , Dan Zhiwu , Zhao Weibo , Xu Wuwu , Huang Wenchao 
Published: December 2022

IntroductionThe highly conserved tubby-like proteins (TLPs) play key roles in animal neuronal development and plant growth. The abiotic stress tolerance function of TLPs has been widely explored in plants, however, little is known about comparative studies of TLPs within crops.MethodsBioinformatic identification, phylogenetic analysis, Cis-element analysis, expression analysis, Cis-element analysis, expression analysis and so on were explored to analysis the TLP gene family of multiple crops.ResultsIn this study, a comprehensive analysis of TLP genes were carried out in seven crops to explore whether similar function of TLPs in rice could be achieved in other crops. We identified 20, 9, 14, 11, 12, 35, 14 and 13 TLP genes in Glycine max, Hordeum vulgare, Sorghum bicolor, Arabidopsis thaliana, Oryza sativa Japonica, Triticum aestivum, Setaria italic and Zea mays, respectively. All of them were divided into two groups and ten orthogroups (Ors) based on amino acids. A majority of TLP genes had two domains, tubby-like domain and F-box domain, while members of Or5 only had tubby-like domain. In addition, Or5 had more exons and shorter DNA sequences, showing that characteristics of different Ors reflected the differentiated function and feature of TLP genes in evolutionary process, and Or5 was the most different from the other Ors. Besides, we recognized 25 cis-elements in the promoter of TLP genes and explored multiple new regulation pathway of TLPs including light and hormone response. The bioinformatic and transcriptomic analysis implied the stresses induced expression and possible functional redundancy of TLP genes. We detected the expression level of 6 OsTLP genes at 1 to 6 days after seed germination in rice, and the most obvious changes in these days were appeared in OsTLP10 and OsTLP12.DiscussionCombined yeast two-hybrid system and pull down assay, we suggested that the TLP genes of Or1 may have similar function during seed germination in different species. In general, the results of comprehensive analysis of TLP gene family in multiple species provide valuable evolutionary and functional information of TLP gene family which are useful for further application and study of TLP genes.
Chitosan treatment reduces softening and chilling injury in cold-stored Hami melon by regulating starch and sucrose metabolism
Authors: Zhang Qin , Tang Fengxian , Cai Wenchao , Peng Bo , Ning Ming , Shan Chunhui , Yang Xinquan 
Published: December 2022

Cold-stored Hami melon is susceptible to chilling injury, resulting in quality deterioration and reduced sales. Pre-storage treatment with chitosan reduces fruit softening and chilling injury in melon; however, the underlying mechanism remains unclear. In this study, Gold Queen Hami melons were treated with 1.5% chitosan solution for 10 min before cold storage at 3°C and then the effect of chitosan was examined on fruit firmness, weight loss, chilling injury, soluble solid content (SSC), pectin, and soluble sugar contents of melon fruit. Also, the enzyme activities and gene expressions related to fruit softening and starch and sucrose metabolism were investigated. Chitosan treatment reduced the fruit softening and chilling injury, maintained the high levels of starch and sucrose contents, and regulated the enzyme activities and gene expressions related to starch and sucrose metabolism. Fruit firmness was significantly positively correlated with sucrose and starch contents. Altogether, we uncovered the potential mechanism of chitosan coating mitigating melon softening and chilling injury through the regulation of starch and sucrose metabolism.
Need strength, perceived need support, stress symptomatology, and performance in the context of oral exams: A typological approach
Authors: Schürmann Linda , Kärner Tobias , Ringeisen Tobias 
Published: December 2022

IntroductionBased on self-determination theory, we investigated whether examinees are classifiable into profiles based on basic need strength and perceived need support that differ in stress parameters and achievement in the context of a standardized oral exam.Methods92 students reported their basic need strength before and perceived need support provided by the examiner once after the exam. Students indicated their emotions and stress perception at four measurement points and we measured their saliva cortisol concurrently, analyzing stress-related changes over time.ResultsLatent class analyses revealed two higher-quality (low/high, high/high) and two lower-quality (low/low, high/low) need strength/need support classes. Physio-affective stress development was typical of exam situations. Higher-quality classes that met or exceeded the needs displayed more beneficial stress and emotion response patterns than lower-quality classes. Gain-related emotions mediated achievement in the higher-quality classes.DiscussionNeed-supportive examiners can promote student well-being and achievement when they succeed in providing high need satisfaction.
ADHD and reification: Four ways a psychiatric construct is portrayed as a disease
Authors: te Meerman Sanne , Freedman Justin E. , Batstra Laura 
Published: December 2022

IntroductionThe descriptive classification Attention-Deficit/Hyperactivity Disorder (ADHD) is often mistaken for a disease entity that explains the causes of inattentive and hyperactive behaviors, rather than merely describing the existence of such behaviors. The present study examines discourse on ADHD to analyze how authors passively and actively contribute to reification—a fallacy in which a concept is represented as a thing existing on its own.MethodsCritical Discourse Analysis and Qualitative Content Analysis of academic textbooks, scientific articles, websites and videos were used to analyze how ADHD is reified.ResultsThe analyses reveal four ways in which inattentive and restless behaviors are presented as an entity by means of the ADHD classification: language choice, logical fallacies, genetic reductionism, and textual silence. First, language choice, such as medical jargon and metaphors aid in representing ADHD as a disease entity. Second, several logical fallacies do the same, including the relatively unknown “ecological fallacy” that refers to the erroneous belief that average group findings, such as average brain size of groups of those with an ADHD classification, can be applied on an individual level. Third, genetic reductionism is often achieved by overstating the results of twin studies and being silent about the disappointing molecular genetic research. Such textual silence is the last identified mechanism of reification and includes instances in which societal factors that affect the ADHD construct are often omitted from texts, thereby obscuring the extent to which ADHD is a limited heuristic.DiscussionIt is essential that discourse communities do not repeat these four ways of reifying behavior and social relations into an alleged entity with the acronym ADHD. The errors and habits of writing may be epistemologically violent by influencing how laypeople and professionals see children and ultimately how children may come to see themselves in a negative way. Beyond that, if the institutional world shaped to help children is based on misguided assumptions, it may cause them harm and help perpetuate the misguided narrative. To counter the dominant, reifying and medicalizing view, guidelines such as the recently published “Dutch ADHD Psychoeducation Guidelines” might be helpful.
The ETNA mission concept: Assessing the habitability of an active ocean world
Authors: Deutsch Ariel N. , Panicucci Paolo , Tenelanda-Osorio Laura I. , Da Poian Victoria , Cho Yun H. , Venigalla Chandrakanth , Mathanlal Thasshwin , Castillo Specia Emiliano , González Peytaví Graciela , Guarriello Andrea , Gunasekara Onalli , Jones Lewis , Krasteva Mariya , Pouplin Jennifer , Villanueva Nicole , Zaref Sam 
Published: December 2022

Enceladus is an icy world with potentially habitable conditions, as suggested by the coincident presence of a subsurface ocean, an active energy source due to water-rock interactions, and the basic chemical ingredients necessary for terrestrial life. Among all ocean worlds in our Solar System, Enceladus is the only active body that provides direct access to its ocean through the ongoing expulsion of subsurface material from erupting plumes. Here we present the Enceladus Touchdown aNalyzing Astrobiology (ETNA) mission, a concept designed during the 2019 Caltech Space Challenge. ETNA’s goals are to determine whether Enceladus provides habitable conditions and what (pre-) biotic signatures characterize Enceladus. ETNA would sample and analyze expelled plume materials at the South Polar Terrain (SPT) during plume fly-throughs and landed operations. An orbiter includes an ultraviolet imaging spectrometer, an optical camera, and radio science and a landed laboratory includes an ion microscope and mass spectrometer suite, temperature sensors, and an optical camera, plus three seismic geophones deployed during landing. The nominal mission timeline is 2 years in the Saturnian system and ∼1 year in Enceladus orbit with landed operations. The detailed exploration of Enceladus’ plumes and SPT would achieve broad and transformational Solar System science related to the building of habitable worlds and the presence of life elsewhere. The nature of such a mission is particularly timely and relevant given the recently released Origins, Worlds, and Life: A Decadal Strategy for Planetary Science and Astrobiology 2023–2032, which includes a priority recommendation for the dedicated exploration of Enceladus and its habitable potential.
Characteristics and Outcome of Post-Transplant Lymphoproliferative Disorders After Solid Organ Transplantation: A Single Center Experience of 196 Patients Over 30 Years
Authors: Vergote Vibeke K. J. , Deroose Christophe M. , Fieuws Steffen , Laleman Wim , Sprangers Ben , Uyttebroeck Anne , Van Cleemput Johan , Verhoef Gregor , Vos Robin , Tousseyn Thomas , Dierickx Daan 
Published: December 2022

Post-transplant lymphoproliferative disorder (PTLD) is a rare but life-threatening complication after transplantation. In this retrospective, monocentric study we aimed to collect real life data regarding PTLD and determine the role of Epstein Barr Virus (EBV) status and year of diagnosis on prognosis. We identified 196 biopsy-proven PTLD after solid organ transplantation (SOT) diagnosed at the University Hospitals Leuven (Belgium) from 1989 to 2019. EBV status was positive in 61% of PTLD. The median overall survival (OS) was 5.7 years (95% CI: 2.99–11.1). Although EBV positivity was not significantly correlated with OS in multivariate analyses (HR: 1.44 (95% CI: 0.93–2.24); p = 0.10), subgroup analysis showed a significantly better median OS for EBV negative post-transplant diffuse large B-cell lymphoma (DLBCL) compared to EBV positive post-transplant DLBCL (8.8 versus 2.5 years respectively; p = 0.0365). There was a significant relation between year of PTLD diagnosis and OS: the more recent the PTLD diagnosis, the lower the risk for death (adjusted HR: 0.962 (95% CI: 0.931–0.933); p = 0.017). In conclusion, the prognosis of PTLD after SOT has improved in the past decades. Our analysis shows a significant relation between EBV status and OS in post-transplant DLBCL.
New Scaling Laws for Pinning Force Density in Superconductors
Authors: Talantsev Evgueni F. 
Published: December 2022

Since the report by Fietz and Webb (Phys. Rev.1968, 178, 657–667), who considered the pinning force density, Fp→=Jc→×B→ (where Jc is the critical current density and B is applied magnetic flux density), in isotropic superconductors as a unique function of reduced magnetic field, BBc2 (where Bc2 is the upper critical field), Fp→ has been scaled based on the BBc2 ratio, for which there is a widely used Kramer–Dew–Hughes scaling law of Fp→B=Fp,maxBBc2p1−BBc2q, where Fp,max, Bc2, p, and q are free-fitting parameters. To describe Fp→B in high-temperature superconductors, the Kramer–Dew–Hughes scaling law has been modified by (a) an assumption of the angular dependence of all parameters and (b) by the replacement of the upper critical field, Bc2, by the irreversibility field, Birr. Here, we note that Fp→ is also a function of critical current density, and thus, the Fp→Jc scaling law should exist. In an attempt to reveal this law, we considered the full Fp→B,Jc function and reported that there are three distinctive characteristic ranges of BBc2,JcJcsf (where Jcsf is the self-field critical current density) on which Fp→B,Jc can be splatted. Several new scaling laws for Fp→Jc were proposed and applied to MgB2, NdFeAs(O,F), REBCO, (La,Y)H10, and YH6. The proposed scaling laws describe the in-field performance of superconductors at low and moderate magnetic fields, and thus, the primary niche for these laws is superconducting wires and tapes for cables, fault current limiters, and transformers.
The Genus Cynometra: A Review of Ethnomedicine, Chemical, and Biological Data
Authors: Sabiha Shabnam , Serrano Rita , Hasan Kamrul , Moreira da Silva Isabel B. , Rocha João , Islam Nurul , Silva Olga 
Published: December 2022

Cynometra L. is a Fabaceae genus that is widely distributed throughout the tropics, consisting of tropical forest trees with ecological and economic importance since they are used as food and herbal medicines by the populations of their natural habitats. Our goal is to provide a review of the research data concerning the potential of this botanical genus as a source of herbal medicines and secondary metabolites that are useful for human health. To that end, scientific databases, including PubMed, Science Direct, ISI Web of Science, Scopus, and Google Scholar, were searched using the following terms: Cynometra, medicine, chemical, biological activity, toxicity, and “AND” as the Boolean connector. Eleven Cynometra species (9.7%) were reported to be used in traditional medicine to treat different ailments. A total of 185 secondary metabolites of various chemical classes, mainly flavonoids and terpenoids, were identified in eight Cynometra species (7.1%). Vitexin was the only flavonoid identified as bioactive in the sequence of bioguided studies on this botanical genus. Ten species (8.8%) were submitted to in vitro and in vivo biological activity assays. The main evaluated activities were in vitro antioxidant, antimicrobial, cytotoxic, and in vivo anti-inflammatory activities, but no human clinical trials or safety data about this genus were found. Cynometra cauliflora and Cynometra ramiflora were the most studied species. The present work confirms the use of Cynometra species as a source of medicinal plants. However, more experimental studies must be conducted to better understand this botanical genus’s usefulness as a source of raw materials for pharmaceutical use.
THE SEMANTIC FEATURE ANALYSIS ON TEACHING VOCABULARY FOR YOUNG LEARNERS
Authors: Dwihastuti Dieni Nurhasanah 
Published: December 2022

ABSTRACT
As vocabulary is a necessary mode of communication, it must be acquired first to be able to master the English language. It is a good idea to start vocabulary classes in elementary school. As their memory is still strong in remembering things, they are ready to learn anything new. However, since English is a second language, teachers will need to employ strategies to have students acquire and memorize new words. This study focuses on linguistic knowledge, that is, semantics, which the teacher uses in the classroom and the learning materials to encourage students’ vocabulary. This is a content analysis of the teacher's approaches to teaching new words to young learners using semantic aspects. This research will be observed through two videos of English-language classrooms. The findings reveal that semantic features such as synonym and antonym pairs, polysemy, and applying in short sentences were used in the classroom when teaching vocabulary. During the class, photos, cassettes, music, and a variety of other learning resources are used. These strategies motivate and encourage students to learn the new vocabulary.
Keywords: vocabulary, instruction, young learner, semantics