bluuebunny/crossref_metadata_2025_split huggingface dataset:
The expression of cellulases in Penicillium janthinellum NCIM 1366 is preceded by reorganization of metabolic and stress-responsive pathways and their associated regulators
Authors: Christopher Meera , Sreeja-R AthiraRaj , Abraham Amith , Gokhale Digambar Vitthal , Pandey Ashok , Sukumaran Rajeev K 
Published: August 2022

Abstract
Cellulase production by fungi is tightly regulated in response to environmental cues, and understanding this mechanism is a key pre-requisite in the efforts to improve cellulase secretion. Based on UniProt descriptions of secreted CAZymes, 13 proteins of the cellulase hyper-producer Penicillium janthinellum NCIM 1366 (PJ-1366) were annotated as cellulases- 4 cellobiohydrolases (CBH), 7 endoglucanases (EG) and 2 beta glucosidases (BGL). Cellulase, xylanase, BGL and peroxidase activities were higher for cultures grown on a combination of cellulose and wheat bran, while EG was stimulated by disaccharides. The BGLs also exhibited a low level of glucose tolerance, with docking studies indicating that they may have different binding sites for the substrate cellobiose and the product glucose, which helps to avoid feedback inhibition. Out of the 758 transcription factors (TFs) differentially expressed on cellulose induction, 13 TFs were identified whose binding site frequencies on the promoter regions of the cellulases positively correlated with their abundance in the secretome. Further, correlation analysis of the transcriptional response of these regulators and TF-binding sites on their promoters indicated that cellulase expression is possibly preceded by up-regulation of 12 TFs and down-regulation of 16 TFs, which cumulatively regulate transcription, translation, nutrient metabolism and stress response.
Cyclosporine A- induces cardiac remodeling through TGF-β/Smad3/miR-29 signaling pathway and gene expression alteration of miR-30b-5p/ CaMKIIδ isoforms pathway: alleviated effect of moderate exercises
Authors: Nourmohammadi Khatereh , bayrami abolfazl , naderi roya , shirpoor alireza 
Published: August 2022

Abstract
Purpose
Cyclosporine A (CsA)-induced cardiac interstitial fibrosis and cardiac hypertrophy is a highly known phenomenon, whereas the basic mechanisms of CsA cardiotoxicity are not clear yet. The present study evaluated the role of the TGF-β/Smad3/miR-29b signaling pathway and CaMKIIδ isoforms gene expression in cardiac remodeling under sole CsA exposure or in combination with moderate exercises.
Methods
A total of 24 male Wistar rats were divided into three groups as follows: control, cyclosporine (30mg/kg BW), and cyclosporine-exercise groups.
Results
After 42 days of treatment, the findings revealed a significant decline in miR-29 and miR-30b-5p gene expression, increases in gene expression of Smad3, calcium/calmodulin-dependent protein kinaseIIδ (CaMKIIδ) isoforms, Matrix Metalloproteins (MMPs), protein expression of TGF-β, heart tissue protein carbonyl and oxidized LDL (Ox-LDL), as well as plasma LDL and cholesterol levels in CsA treated group in comparison to the controls. The CsA group, in comparison to the controls, presented histological heart changes like fibrosis, necrosis, hemorrhage, infiltrated leukocyte, and left ventricular weight/ heart weight. Moderate exercise and CsA, relatively improved gene expression changes and histological alternation in comparison to the CsA group.
Conclusion
These findings suggested that TGF-β-Smad3-miR-29 and CaMKIIδ isoforms may somehow have a main contribution to the progression of heart fibrosis and hypertrophy due to CsA exposure, which provides new insight into the pathogenesis and treatment of CsA induced side effects on heart tissue.
Experimental investigation of material removal in elliptical vibration cutting of cortical bone
Authors: bai wei , Zhai Yuhao , Zhao Jiaqi , Han Guangchao , Ye Linzheng , Zhu Xijing , Shu Liming , Wang Dong 
Published: August 2022

Abstract
To benefit the tissue removal and postoperative rehabilitation, the increased efficiency and accuracy and the reduced operating force are strongly required in osteotomy. A novel elliptical vibration cutting (EVC) has been introduced for the bone cutting compared with the conventional cutting (CC) in this paper. With the assistance of the high-speed micrcope imaging and the dynamometer, the material removals of cortical bone and their cutting forces from two cutting regimes were recorded and analysed comprehensively, which clearly demonstrated the chip morphology improvement and the average cutting force reduction in the EVC process. It also revealed that the elliptical vibration of the cutting tool could promote the fracture propagation along the shear direction. These new findings will be of important theoretical and practical values to apply the innovative EVC process to the surgical procedures of osteotomy.
Design of Intelligent and Connected Vehicle Driving System Based on Digital Twin
Authors: Zhang Hong , Zheng Zan , Yu Hailiang 
Published: August 2022

Abstract
Aiming at the application problem of digital twin technology in the driving scenario of intelligent and connected vehicles, this paper proposes the design scheme of intelligent and connected vehicles driving system based on digital twin. Firstly, the concept of the digital twin, the general establishment method of the twin model, and the design process of the system are introduced; the framework of the driving system of the intelligent and connected vehicles based on the digital twin is proposed based on the technical condition, value demand of the intelligent and connected vehicles, and the architecture of the digital twin. Then, according to the functional requirements of the driving system of the intelligent and connected vehicles, the establishment method of the relevant model of the driving system and the logic of the functional realization are deeply elaborated from three aspects: the entity modeling of the driving elements, the dynamic modeling of the driving process, the simulation modeling of the driving process, and the application of various technologies in the modeling are discussed comprehensively. Finally, it makes an outlook on the development of digital twin technology in the field of intelligent and connected vehicles.
Myelin restoration by antisense modulation of HuR and HuD ELAV RNA binding protein expression in mouse models of multiple sclerosis
Authors: Borgonetti Vittoria , Galeotti Nicoletta 
Published: August 2022

Abstract
Neuropathic pain is the most difficult to treat pain syndrome in multiple sclerosis. Evidence relates neuropathic pain to demyelination that often originates from unresolved neuroinflammation or altered immune response. Post–transcriptional regulation of gene expression might play a fundamental role in the regulation of these processes and the aim of this study was to investigate ELAV RNA binding proteins in demyelination-associated neuropathic pain states. The ubiquitous HuR and the neuronal HuD are involved in inflammatory phenomena and neuronal development and maintenance, respectively. Thus, we investigated their role in the mouse experimental autoimmune encephalomyelitis (EAE) model. HuR resulted overexpressed in the spinal cord of MOG–EAE and PLP–EAE mice and was detected in CD11b+ cells. Intranasal delivery of an antisense oligonucleotide (ASO) to knockdown HuR increased myelin basic protein expression and Luxol Fast Blue staining, indication of increased myelin content, in both EAE models. These effects temporally coincided with attenuation of pain hypersensitivity. Conversely to HuR, HuD was largely downregulated in the MOG-EAE spinal cord, along with GAP43 and Neurofilament H, while in PLP-EAE mice HuD and neuronal markers remained unaltered. In MOG-EAE mice anti–HuR ASO increased the expression of HuD in GAP43 expressing cells and promoted a HuD–mediated neuroprotective activity. In PLP-EAE mice HuR silencing dampened pro–inflammatory response mediated by spinal microglia activation. In conclusion, anti–HuR ASO showed myelin protection at analgesic doses with multitarget mechanisms, and it deserves further consideration as an innovative agent to counteract demyelination in neuropathic pain states.
Evaluating Drug Response in 3D Triple Negative Breast Cancer Tumoroids with High Content Imaging and Analysis
Authors: Sirenko Oksana , Brock Courtney K , Lim Angeline , Macha Prathyushakrishna , Nikolov Ekaterina , Olsen Cathy , McConnell Emily C. , Wright Maryl , Collins-Burow Bridgette M , Cromwell Evan F , Burow Matthew E 
Published: August 2022

Abstract
There is a critical need to develop methods for efficient testing of drug efficacy in patient-derived tumor samples to discover new therapeutics. Two-dimensional (2D) cell culture remains the primary method of drug screening, despite being considered less physiologically relevant than three-dimensional (3D) culture. Increased complexity and technical challenges of 3D systems have limited its widespread adoption as a primary screening method. In this study, we demonstrate methods for increased throughput, imaging and automation in 3D assays that are suitable for compound screening using patient-derived samples. In addition, we show analysis approaches and descriptors that allow gain more information about disease phenotypes and compound effects. We measured responses to drug treatment in 3D tumoroids for cytotoxicity and altered morphology. Tumoroids were formed from primary cells isolated from a patient-derived tumor explant, TU-BcX-4IC, that represents metaplastic breast cancer with a triple-negative subtype and treated with 165 compounds, of approved cancer drugs, at multiple concentrations. We characterize multiple quantitative descriptors for tumor phenotypes and compound effects. Cell Painting method was used for 3D tumoroids for evaluation of phenotypic effects. Eight compounds were detected that demonstrated effects at low concentrations (10nM), including romidepsin, trametinib, bortezomib, carfilzomib, panobinostat, which will be further investigated as potential drug candidates.
Interesting Photodiode Characteristics of Silica Nanoparticles Extracted from Pleurosira laevis Diatom Frustules
Authors: Ashery Adel , El-Fatah Hesham M. Abd , Ali Dina M. , El-Din Rawheya A. Salah , El-Sayed Afaf 
Published: August 2022

Abstract
In this work, we measured the I-V characteristics for silica nanoparticles (SNPs) extracted from the Pleurosira laevis diatom and deposited on top of p-type Si(111) wafer. The electric response of SNPs-based photodiode is found to be more sensitive to thermal and optical powers than the fresh frustules-based photodiode by about 3 and 4 times, respectively. Moreover, they exhibit better photodiode parameters, e.g. ideality factor, series resistance, and shunt resistance. This is stimulating to use the extracted SNPs in innovative electronic and optoelectronic applications as an abundant, cheap, and easy-to-process material.
Job performance and associated factors among health workers working in public hospitals of West Hararghe zone, Oromia Region, Eastern Ethiopia
Authors: Ousman Yassin Abrahim , Worku Biniam 
Published: August 2022

Abstract
Background: The success of any organization always depends on the participation and job performance of their employees. Health workers job performance level are cornerstone for better productivity of any health care organizations Less performance of health workers reduces public hospital productivity and a reason for poor hospitalized patient health outcome. However, little was understood about job performance and associated factors in low-income countries like Ethiopia including the study area.Objectives: To assess the level of job performance and associated factors among health workers working in public hospitals of West Hararghe zone, Oromia Region, East Ethiopia.Methods: A health facility based cross-sectional study design was conducted by using quantitative methods among 422 health workers. The study was conducted from August 27 to September 17, 2018 on public hospitals in West Hararghe zones [Chiro, Galemso and Asebot Hospitals]. Simple random sampling technique was used to select study participants. Data were collected from participants by using self-administered questionnaire. The data were entered in to Epidata version 3.1 and then exported to SPSS version 23 for analysis. Descriptive statistics such as frequency, percentages, mean and standard deviation were used to summarize the data. In bivariate analysis variables which had p-value p<0.25 were taken to multivariate regression model. Multivariate logistic regression was computed to identify predictor variables associated with level of job performance and variables with p ≤ 0.05 were considered statistically significant with 95% CI.Result: A total of 361 study participants were involved in the study with response rate of 86%. Among the respondents, 196 [54.3% were males. In professional types 243 [67.3%] were health professionals. The most predicting variable of health workers job performance: sex [AOR=1.896[1.130, 3.180]], marital status [AOR=1.910 [1.11, 3.27]], working hours [AOR=2.910[1.653, 5.123]], working condition [AOR= 2.164[1.128, 3.844]] and job satisfaction [AOR= 2.480[1.374, 4.476]].Conclusion: The findings our study has indicated that health workers in West Hararghe zone in public hospitals have poor level of job performance. The strong predicting variable such as sex, marital status, working hours, working condition and job satisfaction were significantly associated with job performance. Regional Health office, Zonal health office and hospitals should give more attention on job satisfaction and working condition to improve health worker‟s job performance.
Synthesis of eco-friendly ZnO-based heterophotocatalysts with enhanced properties under visible light
Authors: Mougnol Jean Bedel Batchamen , Waanders Frans , Ntwampe Seteno Karabo Obed , Fosso-Kankeu Elvis , Alili R. Al 
Published: August 2022

Abstract
Heterogeneous photocatalysts have been widely used for the removal of various organic pollutants from wastewater. The main challenge so far resides in the sustainability of the process, with regard to the synthesis and the application under visible light. In this study the precipitated materials from the moringa oleifera seed (MO), groundnut shells (GS) and apatite (A) agrowastes were functionalized with zinc oxide (ZnO) and silver (Ag) solution, to produce novel bioheterophotocatalysts. Various analytical techniques such as scanning electron microscope (SEM), energy-dispersive x-ray spectroscopy (EDS), photoluminescence (PL) and x-ray diffraction (XRD) analyses were used for the characterization of the novel photocatalysts. It was proven that, besides the addition of metals into a semiconductor, agrowastes were also proven to be even better to enhance the photocatalytic activity of a ZnO-based photocatalyst. The combination of MO/GS/A/ZnO/Ag in a 1:1:1 ratio, resulted in a lower band gap of 1.59 eV, as compared to the larger band gap of 2.96 eV for ZnO/ Ag. These photocatalysts' efficiency was also tested on the photodegradation of PAHs derived from coal leaching in various water sources such as acidic mine drainage, alkaline mine drainage and sewage wastewater. From MO/GS/A/ZnO/Ag, the removal efficiency was obtained to be 69.59%, 62.19% and 61.68% while 52.62%, 37.96 and 44.30% using ZnO/Ag in acidic mine drainage, alkaline mine drainage and sewage wastewater in 60 min under solar irradiation. Which happens to highly influence the destabilization of PAHs in the water sources.
Forensic Significance of Perforation Pattern In Polycarbonate Sheet Against 9 × 19mm Projectile
Authors: Prajapati Abhishek , Rohatgi Richa , Kumar Saurabh 
Published: August 2022

Abstract
Polycarbonate is widely used as structural material due to its extreme resistance to impact and perforation. In a crime involving firearms, the bullet may impact various objects fitted with or made of polycarbonate sheets leaving high chances of discovering the fractured PC sheets and fragments at the scene of crime hence these objects may become objects of the criminal investigation. In the present work the perforation pattern of PC sheets of thicknesses 8mm, 10mm, 12mm and 15mm at firing ranges 5m and 10m when impacted against 9×19mm Full Metal Jacket (FMJ) bullet were examined. It was observed that after impact the PC sheet develops entry hole smaller than the calibre of bullet; formation of crack zone, plastic zone; and petalling on the distal face were observed. It was found that as the thickness of the sheet increases, the diameter of the entry hole increases. The diameter of the exit hole initially increases and then becomes constant. At 5m range of fire the trend of crack zone becomes nearly constant from 8mm to 15mm thickness, however, at 10m range of fire crack zone increases from 8mm to 15mm thickness. Furthermore, the diameter of plastic zone increases as the thickness of the sheet increases (from 8mm to 15mm respectively).
Trends and influencing factors of ADRs associated with China’s national essential medicines: A time-series analysis based on data from the National ADR Monitoring Network
Authors: Tang Wan , Chen Hongdou , Zhang Zixin , Wu Gang , Lin Yanquan 
Published: August 2022

Abstract
Objective. To explore the drivers of the increase in adverse drug reactions (ADRs) associated with drugs in China's list of essential medicines and to analyse the ADR impact of the number of visits, based on the recorded annual increase in ADRs associated with essential medicines in China, to provide a reliable theoretical basis for further analysis and optimization of the safety of essential drugs. Methods. A time-series analysis was conducted based on the ADR data for essential medicines from 2011 to 2020 collected from the National ADR Monitoring Center. The relationship between the ADRs associated with essential medicines and the number of visits was explored by means of a vector autoregressive model, Granger causality test, impulse response analysis, variance analysis and other quantitative analysis methods. Results. There was a long-term cointegration relationship and one-way causality between the number of visits and ADRs caused by essential medicines. In the initial stage, the ADR response to the number of visits increased sharply, but with an increase in the number of lag periods, the impact remained basically stable, even showing a slight decreasing trend. Conclusion. The number of visits impacts ADRs caused by essential medicines, but this impact remains basically stable after reaching a certain level. In the long run, the impact may decrease.
Analysis of merged transcriptomic and genomic datasets to identify genes and pathways underlying residual feed intake in growing pigs
Authors: Ibragimov Emil , Pedersen Anni Øyan , Xiao Liang Hanlin , Cirera Susanna , Fredholm Merete , Karlskov-Mortensen Peter 
Published: August 2022

Abstract
Improvement of feed efficiency (FE) in pigs is an important milestone in order to reduce the economic and environmental impact of pig production. The goal of finding biomarkers for FE has been persisting for decades. However, due to the complexity of the FE trait, these goals have still not been met. Here, we search for quantitative trait loci (QTL), candidate genes, and biological pathways associated with FE using both genotype and RNA-seq data. We obtained genotype and colon epithelium RNA-seq data for 371 and 96 pigs, respectively. In total, a genome-wide association study (GWAS) and differential expression (DE) analysis lead to detection of three QTL on SSC9 and 93 DE genes associated with FE. Possible intersection points between genes located in QTL and DE genes were found on levels of transcription factor-target and protein-protein interaction. Moreover, a cis-eQTL analysis revealed associations between genotype and expression levels of 19 DE genes including three genes located in the GWAS QTLs, which may fulfill the connection between genotype and phenotype through DE. Finally, single nucleotide polymorphism calling using RNA-seq data for genes located in RFI QTL revealed 53 polymorphisms, which may include causative mutations.
Synchronous high-amplitude co-fluctuations of functional brain networks during movie-watching
Authors: Tanner Jacob , Faskowitz Joshua , Byrge Lisa , Kennedy Daniel , Sporns Olaf , Betzel Richard 
Published: August 2022

Abstract
Recent studies have shown that functional connectivity can be decomposed into its exact framewise contributions, revealing short-lived, infrequent, and high-amplitude time points referred to as ``events.'' Although events contribute disproportionately to the time-averaged connectivity pattern, improve identifiability and brain-behavior associations, and have been linked to endogenous hormonal fluctuations and autism, their origins remain unclear. Here, we address this question using two independently-acquired imaging datasets in which participants passively watched movies. We find that events synchronize across individuals and based on the level of synchronization, can be categorized into three distinct classes: those that synchronize at the boundaries between movies, those that synchronize during movies, and those that do not synchronize at all. We find that boundary events, compared to the other categories, exhibit greater amplitude, distinct co-fluctuation patterns, and temporal propagation. We show that underlying boundary events is a specific mode of co-fluctuation involving the activation of control and salience systems alongside the deactivation of visual systems. Finally, we find a strong positive relationship between the similarity of time-locked co-fluctuation patterns and the propensity for those time-locked frames to involve synchronous events. Collectively, our results suggest that the spatiotemporal properties of events are non-random and locked to time-varying stimuli.
Ablation of CXCR4 expression in cardiomyocytes exacerbates isoproterenol-induced cell death and heart failure
Authors: cheng min , Chen Can , Yu kunwu , Zeng Qiutang , Zhu Feng 
Published: August 2022

Abstract
Heart failure is an ageing-associated disease. CXCR4, a seven-transmembrane-spanning Gi-coupled receptor for the SDF-1 chemokine, plays a critical role in organogenesis, healthy ageing, vascular post-injury repair and regeneration; however, the specific role of cardiomyocyte CXCR4 in heart function and the pathogenesis of heart failure is incompletely understood. We hypothesize that CXCR4 expression in cardiomyocytes is protective during β-adrenoceptor/Gs stress-induced cardiac dysfunction. Cardiomyocyte-specific CXCR4 knockout (CXCR4-CMKO) mice were generated by crossing CXCR4fl/fl and MHC-Cre+/− mice and shown to display normal cardiac structure and function in the basal state until at least 4 months old. However, following continuous administration of isoproterenol (Iso) via an osmotic mini-pump, their ventricular myocardial contractility, dilation, cardiomyocyte apoptosis, and interstitial fibrosis were worsened compared to that of control MHC-Cre+/− littermates under same treatment. In the cultured H9C2 cardiomyocytes, SDF-1 treatment markedly attenuated Iso-induced apoptosis and reduction in phospho-Akt, and these protective effects were diminished by knockdown of CXCR4 or by co-treatment with Gi inhibitors. Thus, cardiomyocyte SDF-1/CXCR4 signaling alleviates β-adrenergic stress-induced cell death and cardiac adverse remodeling, thus may be harnessed for treatment of heart failure.