bluuebunny/crossref_metadata_2025_split huggingface dataset:
Desenvolvimento da leitura na educação infantil: o papel da ludicidade
Authors: Rodrigues Adriana Maria de Melo , Cordeiro Elizabeth Gomes da Rocha , Moreira Keila Noeme da Penha , Pereira Natália da Silva , Cruz Taís Renata de França , Silva José Eduardo 
Published: January 2022

A pesquisa tem por objetivo refletir e compreender a importância da ludicidade na aquisição da leitura, bem como apresentar meios lúdicos do tipo: literatura, jogos infantis e o brincar, expressões artísticas e práticas musicais. Como instrumentos de aprendizagem nos anos iniciais, descrevendo os benefícios que cada atividade pode proporcionar para os alunos da educação infantil. Discorre-se sobre como o professor pode utilizar os recursos lúdicos em suas práticas pedagógicas, considerando que cada um contribui significativamente para a promoção da aprendizagem efetiva e também para a alfabetização. O foco da pesquisa está na perspectiva sociointeracionista, presente nas experiências que a criança vive por meio das interações com o meio, com outras crianças, com os adultos e consigo mesma. O estudo teve como procedimento metodológico a pesquisa bibliográfica, através da revisão de literatura em fontes de informação como livros, artigos científicos e trabalhos acadêmicos. Pode-se considerar que buscou-se reiterar a importância da ludicidade e dos instrumentos pedagógicos como aliados ao processo de desenvolvimento da leitura no contexto escolar, objetivando oferecer propostas para a viabilização de uma prática pedagógica prazerosa, formativa e saudável para as crianças, através da ludicidade e das múltiplas linguagens infantis.
Estado nutricional de crianças residentes em comunidades rurais: uma revisão integrativa de literatura
Authors: Pereira Thaís da Conceição , Carneiro Yasmin Ventura Andrade , Lima Laurineide Rocha , Cavalcante Edilma Gomes Rocha , Duavy Sandra Mara Pimentel , Damasceno Simone Soares , Beltrão Izabel Cristina Santiago Lemos de 
Published: January 2022

A desnutrição ainda é um desafio para diversas nações, perdurando entre crianças oriundas de comunidades socialmente mais vulneráveis, tais como as comunidades rurais. Tem por objetivo descrever o estado nutricional de crianças residentes em comunidades rurais. Trata-se de uma revisão integrativa de literatura, realizada nos meses de setembro a dezembro de 2020, nas bases de dados Web of Science (WOS). As informações coletadas foram organizadas e compiladas eletronicamente em planilha do programa Microsoft Office Excel®versão2013, sendo validadas por dupla digitação, visando conferir maior confiabilidade. O estado nutricional de desnutrição foi observado na maioria dos estudos, indicando que as crianças em comunidades rurais, com pais não escolarizados, em condições inadequadas de moradia, vivendo em zonas de conflito e vulnerabilidade socioeconômica encontram-se mais susceptíveis à insegurança alimentar infantil. Nesse sentido, salienta-se a relevância de políticas públicas bem estruturadas e sistematicamente efetivas de inclusão social, no combate à pobreza e com foco para a redução da insegurança alimentar, visando promover a disponibilidade, o acesso °ZXX’e o consumo de alimentos ricos em nutrientes.
Uso racional de antimicrobianos e impacto no perfil de resistência microbiológica em tempos de pandemia pela Covid-19
Authors: Mesquita Rafael Ferreira , Lima Cicero Allan Landim de Oliveira , Lima Luan Victor Almeida , Aquino Bruno Pinheiro , Medeiros Melissa Soares 
Published: January 2022

O uso racional de antimicrobianos tem sido uma das principais medidas para evitar o aumento de resistência antimicrobiana no mundo. O objetivo do presente estudo foi avaliar a utilização de antimicrobianos em um hospital privado de Fortaleza/Brasil e correlacionar com a crescente resistência antimicrobiana. Foram determinados dados quantitativos sobre infecções bacterianas, perfil microbiológico e uso de antimicrobianos obtidos de pacientes atendidos nos setores, entre janeiro de 2016 e agosto de 2021. Foram emitidos 2.733 pareceres para avaliação de uso de antibióticos em 2020 e em 2021 foram 2.044. Durante o ano de 2020 a utilização de antimicrobianos foi mais frequente entre maio e junho, e em 2021 nos meses de março e abril, sendo estes referidos meses os que refletem os pacientes com internação prolongada pós-Covid-19 e múltiplas infecções bacterianas secundárias. Observa-se também nesse período maior prescrição de Polimixina B e glicopeptídeos. Ao avaliar o perfil de sensibilidade dos Gram negativos, para P. aeruginosa observa-se uma redução drástica no último ano da sensibilidade a Piperacilina/tazobactam e Carbapenens, bem como a Polimixina B (91,7% de sensibilidade). Para K. pneumoniae os resultados foram ainda piores, evidenciando alta resistência aos Carbapenens e Polimixina B, cuja sensibilidade do último foi de apenas 76,2%, dificultando a escolha de opções terapêuticas. Detectou-se redução da sensibilidade a Cefatzidima/avibactam para P. aeruginosa, K. pneumoniae e Serratia sp. Concluiu-se que o aumento na prescrição de antimicrobianos de amplo espectro durante a pandemia por Covid-19 podem ter impactado no aumento da resistência bacteriana principalmente para Gram negativos.
Sarcoma de Kaposi em pacientes portadores de HIV
Authors: Silva Filho Paulo Sérgio da Paz , Lemos Alaíde Silva , Silva Camila Pinheiro Santiago , Silva Maria Clara Gomes , Carvalho Perla Maria Antão de Alencar , Cardoso Alexandra Rodrigues , Oliveira Juniel Marques de , Silva Fernanda Maria , Penha Ana Alinne Gomes da , Ferreira Samyra Lima , Alencar Suzy Romere Silva de , Cruz Milena Matos da , Esperandio João Vitor Messias , Silva Tálison Vieira da , Serzedo Maria Clara Cronemberger Guimarães , Silva Regina Célia da , Vasconcelos Brenda Steffane Viana , Morais Fares José Lima , Pompeu José Guilherme Férrer 
Published: January 2022

O presente estudo tem como objetivo descrever a doença Sarcoma de Kaposi como uma condição relacionada a pacientes com HIV/AIDS e suas características, além disso o estudo buscou relatar as principais manifestações clinicas da doença, buscando assim, um diagnóstico rápido e efetivo, visando o tratamento para pacientes acometidos pela doença. A metodologia escolhida foi revisão bibliográfica do tipo revisão integrativa da literatura. Para a realização desse estudo, foram consultadas as bases de dados: MEDLINE, Scholar Google, LILACS, SCIELO e Repositórios. Utilizou-se os Descritores em Ciências da Saúde (DeCS) de forma associada: Sarcoma de Kaposi, Vírus da imunodeficiência humana, AIDS, Sintomas e Tratamento. Inicialmente, 205 estudos com a temática proposta; dentre estes, foram excluídos 105 estudos, de acordo com os critérios de exclusão estabelecidos. Após a aplicação dos critérios de inclusão, restaram 12 estudos. O Sarcoma de Kaposi é uma neoplasia pouco prevalente e que há poucos anos era dificilmente vista pela comunidade médica. No entanto, com o aumento da infecção pelo HIV em populações com risco maior de desenvolverem está neoplasia e com os inúmeros diagnósticos tardios da infecção pelo HIV, vem ganhando espaço no sistema de saúde.
Properties and Roles of γδT Cells in Plasmodium yoelii nigeriensis NSM Infected C57BL/6 Mice
Authors: Xie Hongyan , Xie Shihao , Wang Mei , Wei Haixia , Huang He , Xie Anqi , Li Jiajie , Fang Chao , Shi Feihu , Yang Quan , Qi Yanwei , Yin Zhinan , Wang Xinhua , Huang Jun 
Published: January 2022

BackgroundMany kinds of immune cells are involved in malaria infection. γδT cells represent a special type of immune cell between natural and adaptive immune cells that play critical roles in anti-parasite infection.MethodsIn this study, malaria infection model was constructed. Distribution of γδT cells in various immune organs and dynamic changes of γδT cells in the spleens of C57BL/6 mice after infection were detected by flow cytometry. And activation status of γδT cells was detected by flow cytometry. Then γδT cells in naive and infected mice were sorted and performed single-cell RNA sequencing (scRNA-seq). Finally, γδTCR KO mice model was constructed and the effect of γδT cell depletion on mouse T and B cell immunity against Plasmodium infection was explored.ResultsHere, splenic γδT cells were found to increase significantly on day 14 after Plasmodium yoelii nigeriensis NSM infection in C57BL/6 mice. Higher level of CD69, ICOS and PD-1, lower level of CD62L, and decreased IFN-γ producing after stimulation by PMA and ionomycin were found in γδT cells from infected mice, compared with naive mice. Moreover, 11 clusters were identified in γδT cells by scRNA-seq based t-SNE analysis. Cluster 4, 5, and 7 in γδT cells from infected mice were found the expression of numerous genes involved in immune response. In the same time, the GO enrichment analysis revealed that the marker genes in the infection group were involved in innate and adaptive immunity, pathway enrichment analysis identified the marker genes in the infected group shared many key signalling molecules with other cells or against pathogen infection. Furthermore, increased parasitaemia, decreased numbers of RBC and PLT, and increased numbers of WBC were found in the peripheral blood from γδTCR KO mice. Finally, lower IFN-γ and CD69 expressing CD4+ and CD8+ T cells, lower B cell percentage and numbers, and less CD69 expressing B cells were found in the spleen from γδTCR KO infected mice, and lower levels of IgG and IgM antibodies in the serum were also observed than WT mice.ConclusionsOverall, this study demonstrates the diversity of γδT cells in the spleen of Plasmodium yoelii nigeriensis NSM infected C57BL/6 mice at both the protein and RNA levels, and suggests that the expansion of γδT cells in cluster 4, 5 and 7 could promote both cellular and humoral immune responses.
Identification of a New Pathogenicity Island Within the Large pAH187_270 Plasmid Involved in Bacillus cereus Virulence
Authors: Dervyn Rozenn , Kavanaugh Devon W. , Cormontagne Delphine , Glasset Benjamin , Ramarao Nalini 
Published: January 2022

ObjectivesBacillus cereus is responsible for food poisoning and rare but severe clinical infections. The pathogenicity of B. cereus strains varies from harmless to lethal strains. The objective of this study was to characterize three B. cereus isolates isolated from the same patient and identify their virulence potentials.MethodsThree isolates of B. cereus were isolated from various blood samples from a patient who developed sepsis following a central venous catheter infection. The three isolates were compared by WGS, genotyping and SNP analysis. Furthermore, the isolates were compared by phenotypical analysis including bacterial growth, morphology, germination efficacy, toxin production, antibiotic susceptibility and virulence in an insect model of infection.ResultsAccording to WGS and genotyping, the 3 isolates were shown to be identical strains. However, the last recovered strain had lost the mega pAH187_270 plasmid. This last strain showed different phenotypes compared to the first isolated strain, such as germination delay, different antibiotic susceptibility and a decreased virulence capacity towards insects. A 50- kbp region of pAH187_270 plasmid was involved in the virulence potential and could thus be defined as a new pathogenicity island of B. cereus.ConclusionsThese new findings help in the understanding of B. cereus pathogenic potential and complexity and provide further hints into the role of large plasmids in the virulence of B. cereus strains. This may provide tools for a better assessment of the risks associated with B. cereus hospital contamination to improve hygiene procedure and patient health.
A Robust Metabolic Enzyme-Based Prognostic Signature for Head and Neck Squamous Cell Carcinoma
Authors: Mai Zizhao , Chen Huan , Huang Mingshu , Zhao Xinyuan , Cui Li 
Published: January 2022

BackgroundHead and neck squamous cell carcinoma (HNSCC) is still a menace to public wellbeing globally. However, the underlying molecular events influencing the carcinogenesis and prognosis of HNSCC are poorly known.MethodsGene expression profiles of The Cancer Genome Atlas (TCGA) HNSCC dataset and GSE37991 were downloaded from the TCGA database and gene expression omnibus, respectively. The common differentially expressed metabolic enzymes (DEMEs) between HNSCC tissues and normal controls were screened out. Then a DEME-based molecular signature and a clinically practical nomogram model were constructed and validated.ResultsA total of 23 commonly upregulated and 9 commonly downregulated DEMEs were identified in TCGA HNSCC and GSE37991. Gene ontology analyses of the common DEMEs revealed that alpha-amino acid metabolic process, glycosyl compound metabolic process, and cellular amino acid metabolic process were enriched. Based on the TCGA HNSCC cohort, we have built up a robust DEME-based prognostic signature including HPRT1, PLOD2, ASNS, TXNRD1, CYP27B1, and FUT6 for predicting the clinical outcome of HNSCC. Furthermore, this prognosis signature was successfully validated in another independent cohort GSE65858. Moreover, a potent prognostic signature-based nomogram model was constructed to provide personalized therapeutic guidance for treating HNSCC. In vitro experiment revealed that the knockdown of TXNRD1 suppressed malignant activities of HNSCC cells.ConclusionOur study has successfully developed a robust DEME-based signature for predicting the prognosis of HNSCC. Moreover, the nomogram model might provide useful guidance for the precision treatment of HNSCC.
Recursos culturales y naturales potencial de una ruta turística gastronómica
Authors: Pita Lino Alexandra , Santos Moreira Vanessa , Pibaque Pionce Maritza , Toala Baque Gema 
Published: January 2022

Los recursos naturales son elementos de la naturaleza que el ser humano aprovecha para satisfacer sus necesidades en concordancia con los recursos culturales en donde si ha intervenido la mano del hombre desde lo histórico, contemporáneo comercial y no comercial, que complementan el potencial de una ruta turística gastronómica, como herramienta de planificación y desarrollo en función de un producto o de un rasgo característico que es capaz de interesar e incentivar el consumo de un elemento que se da en abundancia. El objetivo de este proyecto es identificar estos recursos mediante un inventario y determinación de las actividades a desarrollar para el diseño de la ruta turística gastronómica de la ciudad de Jipijapa. Su metodología desde un enfoque cualitativo y cuantitativo permitió reforzar la recolección de datos y generalizar los resultados encontrados en cuanto a los recursos naturales y culturales apoyada en la técnica de observación para percibir de manera directa el estado actual del lugar investigado.
The administration of 3% Tamarind (Tamarindus indica) leaves ethanol extract cream inhibited the increase of tyrosinase expression and the amount of skin melanin in male Guinea pigs (Cavia porcellus) skin exposed to UV B
Authors: Prabowo Angelia Ongko , Pangkahila Wimpie , Sriwidyani Ni Putu 
Published: January 2022

Background
The main factor of skin aging is exposure to sunlight, especially UV B rays. Tamarind leaves extract is known to contain tannins, flavonoids, and polyphenols as anti- aging agents. This study was aimed to prove the protective effect of 3% Tamarind leaves (Tamarindus indica) ethanol extract cream in inhibiting skin aging due to exposure to UV B by evaluating the tyrosinase expression and the amount of melanin.

Methods
This was a randomized post-test-only control group design involving 36 male Guinea pigs subjects divided into two groups. The control group received placebo cream, and the treatment group received 3% Tamarind leaves ethanol extract cream. The cream application was given twice a day for two weeks. UV B was given three times a week for two weeks with a total dose of 390 mJ/cm2. Tyrosinase expression was examined by calculating the cell percentage that expresses the enzyme using the immunohistochemical method. The amount of melanin was examined digitally by summing the pixel percentage of melanin in Masson Fontana staining slides. Statistical analysis by the Independent T-test to compare the mean between two groups with p=0.05 significance.

Results
The mean tyrosinase expression and the mean amount of melanin in the control group were higher than in the treatment group. 20.650±4.911% vs 16.067±3.451%, p=0.003 and 10.461±4.916 pixel% vs 7.300±3.111 pixel%, p=0.029, respectively.

Conclusion
Administration of 3% Tamarind leaves (Tamarindus indica) ethanol extract cream inhibited the increase in tyrosinase expression and the amount of skin melanin in male guinea pigs (Cavia porcellus) exposed to UV B.
Stakeholder Mapping dalam Pelaksanaan Community Tourism Collaboratvie Governance (CTCG) di Desa Maron Wonosobo
Authors: Ika Nur Afni  
Published: January 2022

Desa Maron Kecamatan Garung Kabupaten Wonosobo menerapkan konsep Community Tourism Collaborative Governance (CTCG) dalam pengelolaan pariwisata yang ada. Hal ini dilakukan guna mengembangkan potensi serta peningkatan sosial ekonomi di Desa Maron. Namun, dalam pelaksanaanya tidak semua stakeholder mendapatkan peran yang sama dalam pengelolaan pariwisata yang ada di Desa Maron. Sehingga dalam tulisan ini akan dijabarkan bagaimana stakeholders mapping dalam pengelolaan pariwisata yang ada di Desa Maron. Serta hal- hal apa saja yang menjadikan tidak meratanya peran stakeholders dalam mengembangkan pariwisata yang ada di Desa Maron Kecamatan Garung Kabupaten Wonosobo. Metode penelitian yang digunakan adalah deskriptif dengan pendekatan kualitatif. Data yang diperoleh dalam penelitian ini adalah data primer dan sekunder yang diperoleh dari wawancara mendalam serta Focus Group Discussion (FGD) dengan berberbagai stakeholder yang terlibat dalam Community Tourism Collaborative Governance (CTCG). Hasil penelitian menunjukan hal yang menjadikan pembagian peran kurang merata adalah (1) masih adanya tarik ulur kepentingan antar stakeholder terutama dari pihak Dinas Pariwisata dan Kebudayaan Kabupaten Wonosobo dengan beberapa komunitas di sekitar Telaga Menjer sehingga beberapa komunitas tidak ikut serta dalam pengelolaannya. (2) Kepemilikan beberapa obyek daya tarik wisata seperti gethek dan spot selfie masih milik perorangan atau individu. (3) Hilangnya trust masyarakat kepada pemerintah sehingga masyarakat lebih memilih ikut mendukung kegiatan komunitas lokal yang merupakan inisiator juga menjadi penyebab utama kurang meratanya peran dalam pengelolaan pariwisata yang ada di Desa Maron
Corporate Social Responsibility (CSR) dalam Perspektif Kebijakan Strategi Pembangunan Perhutani (RPH) Sukorejo
Authors: Ulfa Febi Alfia 
Published: January 2022

Kemunculan corporate social responsibility (CSR) merupakan alternatif yang patut dikembangkan untuk membagi arah tanggung jawab perusahaan terhadap berbagai persoalan sosial dan lingkungan masyarakat. CSR dapat dijadikan sebuah strategi keberpihakan perusahaan terhadap masyarakat dan lingkungan, serta wujud kepedulian perusahaan untuk menjaga dan melakukan upaya-upaya terhadap kemungkinan munculnya sikap negatif industrialisasi. Perum Perhutani merupakan salah satu BUMN yang telah melaksanakan CSR di berbagai daerah. Salah satunya terdapat pada Dusun Pondok Jeruk tepatnya di areal Perhutani RPH Sukorejo BKPH Sukosari Kecamatan Sumberwringin. Penelitian ini difokuskan pada bagaimana menjawab masalah dengan memanfaatkan CSR dan upaya-upaya yang dilakukan Perum Perhutani untuk meningkatkan ekonomi dan kualitas masyarakat dengan melakukan pembangunan yang diampu oleh Perum Perhutani RPH Sukorejo. Tujuan yang akan dicapai oleh penulis dalam penelitian kali ini adalah untuk mengetahui dan mendeskripsikan bagaimana Corporate Social Responsibility yang dilakukan oleh Perum Perhutani RPH Sukorejo dapat meningkatkan ekonomi dan kualitas masyarakat khususnya bagi masyarakat Dusun Pondok Jeruk. Penelitian ini menggunakan metode penelitian deskriptif kualitatif dengan pengumpulan data wawancara serta penentuan informan dengan menggunakan teknik purposive sampling. Hasil penelitian kali ini menunjukkan bahwa terlaksananya CSR Perum Perhutani RPH Sukorejo pada Dusun Pondok Jeruk dengan melakukan pembangunan TK Tunas Rimba dan juga UMKM Kafe Rimba, sehingga membuat Dusun Pondok Jeruk mengalami kesejahteraan masyarakat yang dibuktikan dengan meningkatnya pendapatan ekonomi dan kualitas masyarakat pada anak-anak usia dini.
Kata kunci: CSR, strategi komunikasi pembangunan, Resort Pengelolaan Hutan (RPH)
Komunikasi Antarbudaya pada Mahasiswa Perguruan Tinggi X (Studi pada Mahasiswa Angkatan 2017)
Authors: Wono Hilda , Bio Amos Mbaroputera Ronald Samuel , Herdono Ismojo , Safitri Bela Ayu 
Published: January 2022

Komunikasi antarbudaya memiliki peran penting dalam proses adaptasi mahasiswa sehingga menciptakan suatu hubungan yang harmonis antara mahasiswa satu sama lain. Contohnya perilaku mahasiswa luar Pulau Jawa yang memiliki semangat untuk berinteraksi dengan mahasiswa yang berasal dari Pulau Jawa hanya karena ingin membangun relasi serta berusaha mendapatkan informasi atau pemahaman yang sesuai dengan budaya maupun bahasa yang ada di Pulau Jawa. Penelitian ini bertujuan untuk mengetahui pesan komunikasi verbal dan hambatan-hambatan yang ditemui dalam komunikasi antarbudaya di kalangan mahasiswa Perguruan Tinggi X angkatan 2017. Penelitian ini dilakukan di Surabaya dengan mahasiswa yang memiliki latar belakang budaya yang berbeda. Metode yang digunakan adalah metode deskriptif kualitatif dengan teknik pengumpulan data berupa wawancara. Adapun informan adalah mahasiswa angkatan 2017 dari luar Pulau Jawa karena mahasiswa angkatan 2017 memiliki ragam asal yang berbeda paling banyak dibandingkan dengan angkatan lainnya. Hasil dari penelitian ini menunjukkan bahwa adaptasi dapat lebih cepat terjadi dikarenakan terdapat persamaan peran atau status dalam proses perkuliahan yaitu sama-sama sebagai seorang mahasiswa serta dorongan dari berbagai pihak.
Kata kunci: komunikasi antarbudaya, adaptasi, deskriptif