bluuebunny/crossref_metadata_2025_split huggingface dataset:
Network Pharmacology-based Analysis on Treatment of Diabetic Retinopathy by Berberine
Authors: Wang Ting-Hua , Li Na , Fu Cong , Chen Jin-Lin , Guo Xiliang 
Published: April 2022

Abstract
The diabetic retinal injury model was established. After treatment with berberine, OCT and water maze were measured, and 10 genes were screened out by 4D-DIA quantitative proteomic analysis. Heat map difference analysis and molecular docking were performed. The key words "diabetic retinal injury" were input in PubMed, and the related genes queried were combined with the results of 10 screened proteome analysis to conduct PPI protein interaction. Then GO and KEGG analyses were performed with the interacting genes. Results :OCT results showed that the outer nucleus, inner layer and outer accessory layer of RPE were thickened after berberine treatment. The results of water maze experiment showed that after berberine treatment, the learning and cognitive function of BBR group rats was significantly higher than that of DM group on day 5. By screening proteome analysis data and making Venny cross map, 10 genes could be obtained and heat map could be made. Dennd1a and Utp6 were down-regulated in diabetes group. Atp7a, Ppl, Ogn, Nsmcel, Mme, Lmo4, Ca1 and Fn1 were upregulation, and the berberine treatment group was opposite to the diabetic group. Molecular docking results showed that berberine and berberine could form stable molecular binding patterns except Fnl, Ogn and Utp6 target proteins. 343 genes were searched through PubMed keywords, and 353 genes were combined for PPI protein interaction, and 331 genes were obtained for mutual relationship, which were analyzed by GO and KEGG. Conclusion: Berberine has protective effect on diabetic retina injury.
Challenges in introducing innovation. Barriers and enablers to the WHO Surgical Safety Checklist at the University Teaching Hospital in Lusaka, Zambia: a qualitative study
Authors: Munthali Judith , Pittalis Chiara , Bijlmakers Leon , Kachimba John , Cheelo Mweene , Brugha Ruairi , Gajewski Jakub 
Published: April 2022

Abstract
Background
Surgical perioperative deaths and major complications are important contributors to preventable morbidity, globally and in sub-Saharan Africa. The surgical safety checklist (SSC) was developed by WHO to reduce surgical deaths and complications, through utilizing a team approach and a series of steps to ensure the safe transit of a patient through the surgical operation. This study explored enablers and barriers to the utilization of the Checklist at the University Teaching Hospital (UTH) in Lusaka, Zambia.
Methods
A qualitative case study was conducted involving members of surgical teams (doctors, anaesthesia providers, nurses and support staff) from the UTH surgical departments. Purposive sampling was used and 16 in-depth interviews were conducted between December 2018 and March 2019. Data were transcribed, organized and analysed using thematic analysis.
Results
Analysis revealed variability in implementation of the SSC by surgical teams, which stemmed from lack of senior surgeon ownership of the initiative, when the SSC was introduced at UTH five years earlier. Low utilization was also linked to factors such as: negative attitudes towards it, the hierarchical structure of surgical teams, lack of support for the SSC among senior surgeons and poor teamwork. Further determinants included: lack of training opportunities, lack of leadership and erratic availability of resources. Interviewees proposed the following strategies for improving SSC utilisation: periodic training, refresher courses, monitoring of use, local adaptation, mobilizing the support of senior surgeons and improvement in functionality of the surgical teams.
Conclusion
The SSC has the potential to benefit patients; however, its utilisation at the UTH has been patchy, at best. Its full benefits will only be achieved if senior surgeons are committed and managers allocate resources to its implementation. The study points more broadly to the factors that influence or obstruct the introduction and effective implementation of new quality of care initiatives.
All-optical efficient measurement of the Verdet Constant in achiral and chiral liquid solutions: towards all-optical Magnetic-Spectroscopies
Authors: Rodriguez Vincent , Verreault Dominique , Adamietz Frédéric , Kalafatis Apostolos 
Published: April 2022

Abstract
Magneto-optical properties are wide and span various applications such as magnetic field sensing, imaging technologies, security encoding... In this work, we have revisited the all-optical Inverse Faraday effect method to measure the Faraday rotation angle and determine the Verdet constant of diamagnetic/paramagnetic liquid solutions. We show that using a picosecond laser source the Inverse Faraday effect enables to reach magnetic flux densities in liquids in the range of 1-100 T with average power ranging below 10 mW, levels which are comparable or higher than those obtained using electromagnets to generate pulsed magnetic fields. This all-optical method with no pump-probe scheme has the advantage that it requires small volumes (<100 µl) and enables in situ fast and efficient static measurements. We further show it is possible to determine the Verdet constant of solutes in binary diluted solutions and we also emphasize that chiral molecules exhibit considerable enantiomeric difference .
Genome-wide SNP discovery, identification of QTLs and candidate genes associated with morpho-physiological and yield related traits for drought tolerance in maize
Authors: Sarkar B. , Varalaxmi Y. , Vanaja M. , Kumar N. Ravi , Prabhakar M. , Jyothilakshmi N. , Yadav S. K. , Maheswari M. , Singh V. K. 
Published: April 2022

Abstract
Drought is one of most important abiotic stresses affecting maize yield. The present study was aimed to map genomic regions associated with the morpho-physiological and yield related traits in maize under well-watered and water-deficit stress conditions using recombinant inbred population (RILs) derived from the cross HKI161 × SNJ201126 by genome-wide SNP discovery using genotyping-by-sequencing approach. Phenotyping of RILs showed normal frequency distribution for most of the traits indicating their polygenic nature. The SNP linkage map was generated using 1241 SNP markers which were distributed over 10 linkage groups and covered a total genetic distance of 5471.55cM. A total of 40 QTLs were identified for the various morpho-physiological and yield related traits under well-watered (26) and water-deficit stress conditions (14). Of these 14 were major QTLs, 26 were minor QTLs and two QTLs (qCW2-1, qCH1-1) were detected under both conditions. Three QTL hotspots regions were identified, one for net CO2 assimilation rate (AN) and transpiration rate (TR) (qAN3-1, qTR3-1 and qAN3-2) on chromosome (chr) 3, cob height (CH) and plant height (PH) (qCH8-1 and qPH8-1) on chr 8 and cob weight (CW), total biomass (TB) and grain yield (GY) (qCW2-1, qTB2-1, qTB2-2 and qGY2-1) on chr 2 capturing phenotypic variability ranging between 10.10–22.33%. The QTL hotspot region for AN & TR encoded for genes involved in signal transduction pathway under water-deficit stress tolerance, maintenance of plant membrane and cell wall integrity and cross membrane transport. While QTL hotspot for PH and CH encoded genes involved in carbon utilization in plant growth and development, directed movement of proteins in the cell and transmembrane transporter activity. The QTL hotspot for yield encoded genes involved in signal transduction and plant development. These regions can be further fine mapped and used for marker assisted breeding in maize.
Single Coronary Ostium with Obstructive Hypertrophic Cardiomyopathy-Case Report
Authors: Lv Xin , Li Jianhua , Liu Chuanzhen , Wei Ruyuan , Meng Lingwei , Kong Xiangjin , Wei Kaiming , Cao Guangqing , Liu Kai 
Published: April 2022

Abstract
BackgroundHypertrophic cardiomyopathy (HCM) is the monogenic inherited cardiovascular disorder. In addition, single coronary artery (SCA) is a rare congenital anomaly of the coronary arteries. However, single coronary artery concomitant with severe obstructive hypertrophic cardiomyopathy has never been reported in the literature.Case presentationHerein, we present a 64-year-old female diagnosed as a single left coronary artery with severe obstructive hypertrophic cardiomyopathy. Obstructive hypertrophic cardiomyopathy was treated with "MORROW procedure". The patient was discharged on the seventh postoperative day and has been asymptomatic during the follow-up. ConclusionTo our knowledge, single left coronary artery with severe obstructive hypertrophic cardiomyopathy is the first to be reported in the literature. In addition, myocarial pretection by cardioplegia antegrade perfusion in the single coronary artery was safe in the patient with single coronary artery and obstructive hypertrophic cardiomyopathy.
Does adding the drug-drug similarity to drug-target interaction prediction methods make a noticeable improvement in their efficiency?
Authors: Hassanzadeh Reza , Shabani-Mashcool Soheila 
Published: April 2022

Abstract
Predicting drug-target interactions has become an important bioinformatics issue because it is one of the critical and preliminary stages of drug repositioning. Therefore, scientists are trying to develop more accurate computational methods for predicting drug-target interactions. These methods are usually based on machine learning or recommender systems and use biological and chemical information to improve the accuracy of predictions. In the background of these methods, there is a hypothesis that similar drugs have similar targets. So, the similarity between drugs as chemical information is added to the computational methods to improve the prediction results. The question that arises here is whether this claim is actually true? If so, what method should we use to calculate drug-drug similarities? Will we obtain the same improvement from any method we use?Here, we want to investigate this claimed positive effect. For this purpose, we consider different types of real similarities, random similarities, four important datasets and four state-of-the-art methods. Our results show that the type of data, the method which is used to predict the interactions, and the algorithm used to calculate the similarities are all important, and it cannot be easily stated that adding drug-drug similarities can significantly improve the results. Therefore, our results could suggest a checklist for scientists who want to improve their machine learning methods.
GSK-3β inhibitors can rescue neurons through the prosurvival(autophagy) mechanism in Parkinson’s Disease
Authors: Joshi Deepika , Gupta Garima , Narayan Gopeshwar , Mishra Vijaya Nath , Chaurasia RameshwarNath , Pathak Abhishek , Singh Varun Kumar , Kumar Anand 
Published: April 2022

Abstract
Parkinson’s disease (PD) is a chronic progressive neurodegenerative disorder. The underlying molecular mechanism lead to α-synuclein aggregation, progressive and selective loss of dopaminergic neurons, and intracellular inclusions (known as Lewy bodies (LBs)). Several genetic and environmental factors may lead to PD. Ecological factors can influence the onset of most sporadic PD cases via genetically mediated pathways. Rotenone (a mitochondrial complex one inhibitor, a naturally occurring toxin, a pesticide) reproduces PD’s neurochemical, neuropathological, and behavioral features. Rotenone increased aggregation and phosphorylation of α-synuclein and attenuated the phosphorylation of AKT and GSK3beta. GSK-3β /AKT is involved in multiple signaling pathways. The results suggest that the AKT /GSK3beta signaling pathway mediated the rotenone-induced a-synuclein accumulation. The inhibition of GSK-3β by lithium results in the increase of β-catenin levels to enhance dopaminergic cell viability and then the reduction of accumulation and phosphorylation of α-synuclein by promoting autophagy Lithium inhibit neuronal apoptosis via increasing the BCl2 protein level, Preservation of mitochondrial function with LiCl treatment confirmed that the damaged mitochondria were cleared through autophagy (mitophagy). Via increased level of LC3. Finally, we can conclude that GSK-3beta inhibitors can provide new insights into biology and a possible therapeutic avenue for further investigating Parkinson’s disease.
The role of references and the elusive nature of the chemical bond
Authors: Martín Ángel , Francisco Evelio 
Published: April 2022

Abstract
Chemical bonding theory is of utmost importance to chemistry. We argue that there is still plenty of unexplored territory, and that both internal and external reference biases remain in the standard model of the chemical bond. The former affect the basic notion of wavefunction interference, which is purportedly recognized as the most basic bonding mechanism. The latter influence how bonding models are chosen. After unveiling these different biases, we advocate for the use of real space analyses, which are as reference-less as possible. Delocalisation emerges as the reference-less equivalent to interference and the ultimate root of bonding. Atoms (or fragments) in molecules should be understood as a statistical mixture of components differing in electron number, spin, etc.
Oxygen-Glucose Deprivation (OGD) decreases the motility and length of Axonal Mitochondria in Cultured Dorsal Root Ganglion Cells of Rat
Authors: Kikuchi Shin , Kohno Takayuki , Kojima Takashi , Tatsumi Haruyuki , Ohasaki Yuki , Ninomiya Takafumi 
Published: April 2022

Abstract
Controlling axonal mitochondria is important for their neural network to keep normal functions. Oxygen-glucose deprivation (OGD), mimicking ischemia model, finally induces neuronal cell death as same as axonal degeneration. Axonal mitochondria are disrupted in process in neural degeneration induced by OGD, but the mechanism of mitochondria dysfunctions has not been fully understood. We focused on dynamics of mitochondria in axons exposed to OGD and the number of motile mitochondria significantly reduced in 1 hour after OGD exposure. Further, we found the length of stationary mitochondria decreases which was reasoned by three patterns how stationary mitochondria size decrease; first, stop of motile mitochondria: second, fission of longer stationary mitochondria: third, changing from tubular to sphere mitochondria in axons exposed to OGD. Our study shows that OGD exposure reduces the number of motile mitochondria earlier than stationary mitochondria fragmentation and provides three reasons why stationary mitochondria decrease. Our result suggests that mitochondria are new target for neuroprotective strategy to prevent neuro degeneration induced by ischemia.
Chitosan-coated selenium nanoparticles inhibit porcine reproductive and respiratory syndrome virus (PRRSV) replication by attenuating PRRSV-induced apoptosis via the ROS/JNK signaling pathway in Marc-145 cells
Authors: Shao Chunyan , Yu Ziwei , Luo Tongwang , Zhou Bin , Song Quanjiang , Li Zhuoyue , Yu Xiaoqiang , Jiang Sheng , Zhou Yingshan , Dong Wanyu , Zhou Xingdong , Wang Xiaodu , Song Houhui 
Published: April 2022

Abstract
Background
Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that can cause significant economic losses to the global swine industry. Host-targeted antiviral drugs have been developed as a new strategy for antiviral therapy. In this regard, selenium nanoparticles (SeNPs) have attracted increasing attention in the biomedical field, given their antiviral effects. The purpose of this study was to investigate whether chitosan-coated SeNPs (CS-SeNPs) could affect PRRSV replication in vitro.
Methods
In this study, CS-SeNPs were synthesized by chemical reduction and characterized by assessing the morphology, size distribution, zeta potential, and element composition. Marc-145 cells were infected with r-PRRSV-EGFP (0.1 MOI) and inoculated with CS-SeNPs (10 µM). Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits. ORF5 RNA expression, viral titer, nucleocapsid (N) protein expression were assessed using qRT-PCR, TCID50, and Western blot. ROS generation, apoptosis rates, and JNK /caspase-3/PARP protein expression were evaluated using dihydroethidium staining, flow cytometry, and Western blot.
Results
The results showed that CS-SeNPs treatment significantly suppressed oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis. CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells. The apoptosis rates initially increased by r-PRRSV-EGFP infection were significantly decreased by CS-SeNPs inoculation by inhibiting ROS generation, JNK phosphorylation levels, cleavage of caspase-3, and PARP mainly at 48 hpi.
Conclusions
Taken together, the present data demonstrated that CS-SeNPs synthesized by chemical reduction could enhance antioxidant capacity and effectively suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication. This finding expands the treatment landscape for combating infections caused by PRRSV.
Safety and Efficacy of Immune Checkpoint Inhibitors in Advanced Cancer patients with Autoimmune Disease: a Meta-Analysis
Authors: Cai Qi , Huo Gengwei , Zhu Fuyi , Yue Ping , Yuan Dongqi , Chen Peng 
Published: April 2022

Abstract
Background
Cancer patients with autoimmune disease (AID) usually have been excluded from the clinical trials of immune checkpoint inhibitors (ICIs). The safety and effectiveness of ICIs for these patients remain uncertainty.
Methods
A systematic search was conducted in available electronic database. We collected the incidence of immune-related adverse events (irAEs), progression-free survival (PFS) and overall survival (OS) from included studies.
Results
11 studies including 5489 participants were incorporated in our meta-analysis. The pooled risk ratio (RR) for any grade and grade ≥ 3 irAEs were 1.79 (95% CI: 1.31–2.45) and 1.58 (95% CI: 1.06–2.36), respectively. When irAEs were from the same system as AID, we called the irAEs as AID-homogeneous irAEs, otherwise as AID-heterogeneous irAEs. Subgroup analysis found that the higher risk of AID-homogeneous irAEs contributed to the higher risk of overall irAEs of AID patients. The pooled hazard ratio (HR) for PFS and OS were 1.09 (95% CI: 0.96–1.25) and 1.10 (95% CI: 0.68–1.77), respectively. The results of subgroup analyses for PFS and OS matched the overall results well.
Conclusion
AID patients were significantly associated with a higher risk of irAEs in any grade and ≥ 3 grade under ICIs therapy. Specifically, AID-homogeneous irAEs but AID-heterogeneous irAEs were higher in AID patients than without AID patients. No statistically significant difference in PFS and OS were observed in patients with and without AID.
Dual-phase-lag theory on reflection thermoelastic waves from a rotating gravitation elastic half-space
Authors: Abo-Dahab S. M. , Othman Mohamed I. A. , Abd-Alla A. M. , Kilany A. A. 
Published: April 2022

Abstract
In this research, the theory of generalized thermoelasticity is used to investigate the plane harmonic waves reflection from a rotating semi-infinite elastic solid with a gravity field. The coefficients of reflection, which are the reflected wave amplitudes ratios to incident wave amplitudes, are calculated. The numerical solution is obtained and the graphic representation of the rotation and gravity fields of the on the coefficient of reflection is shown. In the presence and absence of rotation and gravity, comparisons are conducted with the predictions of the Lord-Shulman and dual-phase-lag models. The gravitational and rotational effects explain the reflection coefficient of waves on the free half-space surface.
Investigation of the relationship between a healthy lifestyle, self-esteem and subjective vitality of the elderly in Sirjan in 2020
Authors: Abolghaseminejad Parniya , Borhaninejad vahidreza , Khanjani Narges , Saber Maryam , Alizadeh Somayeh 
Published: April 2022

Abstract

Introduction: This study was conducted aimed to determine the relationship between a healthy lifestyle, self-esteem and subjective vitality of the elderly in Sirjan in 2020.Method: In this descriptive-analytical study, 200 elderly people aged 60 years and older were selected from comprehensive health centers in Sirjan by two-stage random sampling method. The data were collected using validated Healthy Lifestyle Questionnaire among Iranian Elderly, Rosenberg Self-Esteem Scale and validated Subjective Vitality Scale.Results: The mean and standard deviation of healthy lifestyle score was 134.42±13.78, 36.34±4.62 for self-esteem and 21.80±2.85 for subjective vitality. A direct and significant relationship was between healthy lifestyle and self-esteem (p <0.001) and between healthy lifestyle and subjective vitality (p <0.001).Conclusion: A direct and significant relationship was between healthy lifestyle, self-esteem and subjective vitality of the elderly. Therefore, the elderly should take care of their health and benefit from self-esteem and vitality by selecting a healthy lifestyle.
Real-Time Ultrasound Elastographic Features and Color Doppler imaging of Mitral Valve Prolapse
Authors: Topal Dursun , Can Mehmet Erol , Tekin Evrim Karadağ , Uğuz Berat , Kocamaz Mehmet Fatih , Aslanci Mehmet Emin 
Published: April 2022

Abstract
Purpose: To investigate the elasticity of ocular structures in patients with mitral valve prolapse (MVP) Methods: This prospective study included a total of 35 patients with MVP (study group) and 35 healthy volunteers (control group). The elastography value of the ratio of orbital fat- sclera (ROF/S) was measured with real-time US elastography. For each eye, central retinal artery (CRA), posterior ciliary artery (PCA), and ophthalmic artery (OA) were evaluated, respectively. All examinations were performed by the same physician. Results: The mean ages of the patients in the study and the control groups were 31.77 ± 11.40 years, and 30.65 ± 7.45 years, respectively (P =0.511). Mean ROF/S were 1.95 ± 0.81 and 1.37 ± 1.06 (P=0.001) in the study groups and control, respectively. The mean RI of the OA was 0.67 ± 0.05 in the control group, 0.67 ±0.05 (0.55; 0.87) in study group. The mean RI of the PCA was 0.66 ± 0.05 in the control group, 0.68 ±0.06 in study group. . The mean RI of the CRA was 0.66 ± 0.05 in the control group, 0.66 ±0.06 in study group. The RI value was not a significant difference between control and study group (p > 0.05).Conclusion: Scleral elasticity was significantly increased in MVP patients. These could be related to ocular pathologies such as glacouma, kerataconus in MVP.