bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel prognostic predictor of haemoglobin–platelet index in diffuse large B‐cell lymphoma, not otherwise specified: Anaemia and thrombocytopenia are associated with IL‐6 production in lymphoma cells
Authors: Taniguchi Takahide , Nakayama Shoko , Tanaka Hirokazu , Rai Shinya , Hirase Chikara , Morita Yasuyoshi , Tatsumi Yoichi , Ashida Takashi , Matsuda Mitsuhiro , Hashimoto Shigeo , Matsumura Itaru 
Published: April 2022

SummaryWe previously reported that a novel haemoglobin–platelet index (HPI) based on anaemia and thrombocytopenia was useful to predict the prognosis of patients with diffuse large B‐cell lymphoma not otherwise specified (DLBCL NOS). Here, we analyse the utility of HPI in a new validation cohort with DLBCL NOS (n = 94). As a result, we confirm that HPI was effective for differentiating progression‐free survival (PFS) and overall survival in this validation cohort. So, we further compare the utility of HPI with previously reported prognostic markers such as the National Comprehensive Center Network‐International Prognostic Index (NCCN‐IPI), Glasgow prognostic score (GPS), and platelet‐albumin (PA) score, using a larger number of 160 patients consisting of the derivation cohort (n = 66) and a validation cohort (n = 94). As a result, the patients with a higher HPI score had significantly worse outcomes, and HPI predicted the prognosis of DLBCL NOS independently of NCCN‐IPI. HPI was more sensitive than GPS and almost the same as PA score in predicting PFS. Moreover, the patients whose lymphoma cells were positive for interleukin‐6 (IL‐6) (75/111 cases) judged by immunohistochemical staining had significantly lower haemoglobin levels and platelet counts than IL‐6‐negative cases (36/111 cases), suggesting the involvement of IL‐6 produced by lymphoma cells in anaemia and thrombocytopenia in DLBCL NOS patients.
Uptake of planning as a self‐regulation strategy: Adolescents’ reasons for (not) planning physical activity in an intervention trial
Authors: Renko Elina , Kostamo Katri , Hankonen Nelli 
Published: April 2022

AbstractObjectivesPlanning is an effective self‐regulation strategy. However, little is known why some people take up planning and some do not. Such understanding would help interventions to promote planning. We investigated how adolescents explain their (non) use of planning for physical activity after an intervention.MethodsQualitative content analysis was employed to investigate follow‐up interviews (a purposeful sampling; n = 19 low‐to‐moderately active, vocational school students) of Let's Move It trial participants twice post‐intervention: 6–8 weeks and 14 months post‐baseline. In the intervention, planning was one of the key techniques used to promote PA.ResultsWe identified seven categories linked to reasons for (not) using planning. Most were related to feelings anticipated to result from planning. Action‐ and identity‐related concerns were also raised. The reasons for planning were that the plan (1) helps to clarify what to do and to get things done, (2) strengthens the feeling of autonomy, (3) promotes a sense of progress, ability and control over one's PA. The reasons for not planning were that (having) a plan may (1) feel forced and like an unpleasant duty, (2) take away life's spontaneity and freedom, (3) result in anticipated annoyance and bad mood if one fails to enact the plan, or (4) be an effective strategy for others but not for the interviewee.ConclusionsPlanning may not only link to behavioural control but also the sense of autonomy, and thus subsequent motivation. We suggest various strategies to promote planning, including challenging non‐planner identity and harnessing social dimension of planning.
Contact allergy in children with and without atopic dermatitis: An Italian multicentre study
Authors: Bonamonte Domenico , Hansel Katharina , Romita Paolo , Fortina Anna Belloni , Girolomoni Giampiero , Fabbrocini Gabriella , Patruno Cataldo , Napolitano Maddalena , Patrizi Annalisa , Argenziano Giuseppe , Micali Giuseppe , Calzavara Pinton Piergiacomo , Foti Caterina , Stingeni Luca ,   Italian Society of Dermatology and Venereology, Pediatric Dermatology Group
Published: April 2022

AbstractBackgroundContact allergy and atopic dermatitis (AD) are both common inflammatory T cell‐mediated diseases and many factors may influence the prevalence of contact allergy in AD patients. In children, their possible correlation was debated with conflicting results.ObjectivesThe present study aimed to assess the prevalence of contact sensitivity in children and to investigate the association with AD.Materials and methodsA retrospective multicentre study on children aged from 0 to 14 years patch tested between January 2017 and December 2018 was performed. Children were consecutively patch tested with the SIDAPA (Società Italiana Dermatologia Allergologica Professionale Ambientale) baseline series.ResultsAmong the 432 children investigated for contact allergy, 125 (28.9%) showed a positive reaction to at least one of the allergens tested, with a higher prevalence of positive patch test reactions in girls (32.3%) than in boys (25.0%). The most frequent contact allergens were nickel sulphate (10.2%), cobalt chloride (6.7%), methylisothiazolinone (3.7%), fragrance mix‐2 (3.2%), potassium dichromate (2.8%), fragrance mix‐1 (2.1%) and methylchloroisothiazolinone/methylisothiazolinone (2.1%). One‐hundred‐three children (23.8%) suffered from AD showing a higher prevalence of positive patch test (36.9%) compared to children without AD (26.4%).ConclusionsDespite the topic being still controversial, the present study suggests a consistent prevalence of contact allergy among children with higher sensitivity rate among children with AD than without AD.
TrBase: A genome and transcriptome database of Temnopleurus reevesii
Authors: Kinjo Sonoko , Kiyomoto Masato , Suzuki Haruka , Yamamoto Takashi , Ikeo Kazuho , Yaguchi Shunsuke 
Published: April 2022

AbstractSea urchins have a long history as model organisms in biology, but their use in genetics is limited because of their long breeding cycle. In sea urchin genetics, genome editing technology was first established in Hemicentrotus pulcherrimus, whose genome has already been published. However, because this species also has a long breeding cycle, new model sea urchins that are more suitable for genetics have been sought. Here, we report a draft genome of another Western Pacific species, Temnopleurus reevesii, which we established as a new model sea urchin recently since this species has a comparable developmental process to other model sea urchins but a short breeding cycle of approximately half a year. The genome of T. reevesii was assembled into 28,742 scaffold sequences with an N50 length of 67.6 kb and an estimated genome size of 905.9 Mb. In the assembled genome, 27,064 genes were identified, 23,624 of which were expressed in at least one of the seven developmental stages. To provide genetic information, we constructed the genome database TrBase (https://cell-innovation.nig.ac.jp/Tree/). We also constructed the Western Pacific Sea Urchin Genome Database (WestPac‐SUGDB) (https://cell-innovation.nig.ac.jp/WPAC/) with the aim of establishing a portal site for genetic information on sea urchins in the West Pacific. This site contains genomic information on two species, T. reevesii and H. pulcherrimus, and is equipped with homology search programs for comparing the two datasets. Therefore, TrBase and WestPac‐SUGDB are expected to contribute not only to genetic research using sea urchins but also to comparative genomics and evolutionary research.
Trait‐based characterisation of parasitoid wasp communities in natural and agricultural areas
Authors: Kishinevsky Miriam , Keasar Tamar 
Published: April 2022

Abstract
Life‐history traits are increasingly used to understand how arthropod communities assemble and function under diverse conditions, for example why some species are better adapted to agricultural intensification than others. We aimed to understand which traits characterise parasitoid wasps under agricultural disturbances. To this end, we studied parasitoid communities from pomegranate orchards and nearby natural habitats in Israel.
Ten sites along a climate gradient were sampled thrice along one fruit‐growing season. We compiled information on life‐history traits associated with development, adult diet and host taxa, for 27 well‐represented parasitoid species. We tested for relationships between the parasitoids' abundances, functional traits and environmental conditions, using RLQ and fourth‐corner analyses.
Life‐history traits were highly related to environmental variables. Koinobionts (wasps whose parasitized hosts feed and grow), and parasitoids of aphids and whiteflies, were more common, and sugar‐feeding was less common, in orchards than in natural habitats. Parasitism of larval hosts correlated with aridity, while egg parasitism increased with herbaceous vegetation cover.
Host composition and koinobiosis shape parasitoid communities in the orchards. Koinobiosis is often associated with life‐history traits such as small eggs, short life‐span, early egg maturation and high fecundity, which may be adaptive in the frequently disturbed orchard habitats. Further, dense vegetation conditions seem to favour egg parasitism (perhaps because of reduced risks of egg desication), while larval parasitism is more common in arid seasons and sites. These findings provide initial insights regarding the effects of land use and climate on the functional characteristics of parasitoid communities.
Hippocampal position and orientation as prognostic biomarkers for posttraumatic epileptogenesis: An experimental study in a rat lateral fluid percussion model
Authors: De Feo Riccardo , Manninen Eppu , Chary Karthik , Hämäläinen Elina , Immonen Riikka , Andrade Pedro , Ndode‐Ekane Xavier Ekolle , Gröhn Olli , Pitkänen Asla , Tohka Jussi 
Published: April 2022

AbstractObjectiveThis study was undertaken to identify prognostic biomarkers for posttraumatic epileptogenesis derived from parameters related to the hippocampal position and orientation.MethodsData were derived from two preclinical magnetic resonance imaging (MRI) follow‐up studies: EPITARGET (156 rats) and Epilepsy Bioinformatics Study for Antiepileptogenic Therapy (EpiBioS4Rx; University of Eastern Finland cohort, 43 rats). Epileptogenesis was induced with lateral fluid percussion‐induced traumatic brain injury (TBI) in adult male Sprague Dawley rats. In the EPITARGET cohort, ‐weighted MRI was performed at 2, 7, and 21 days and in the EpiBioS4Rx cohort at 2, 9, and 30 days and 5 months post‐TBI. Both hippocampi were segmented using convolutional neural networks. The extracted segmentation mask was used for a geometric construction, extracting 39 parameters that described the position and orientation of the left and right hippocampus. In each cohort, we assessed the parameters as prognostic biomarkers for posttraumatic epilepsy (PTE) both individually, using repeated measures analysis of variance, and in combination, using random forest classifiers.ResultsThe extracted parameters were highly effective in discriminating between sham‐operated and TBI rats in both the EPITARGET and EpiBioS4Rx cohorts at all timepoints (t; balanced accuracy > .9). The most discriminating parameter was the inclination of the hippocampus ipsilateral to the lesion at t = 2 days and the volumes at t ≥ 7 days after TBI. Furthermore, in the EpiBioS4Rx cohort, we could effectively discriminate epileptogenic from nonepileptogenic animals with a longer MRI follow‐up, at t = 150 days (area under the curve = .78, balanced accuracy = .80, p = .0050), based on the orientation of both hippocampi. We found that the ipsilateral hippocampus rotated outward on the horizontal plane, whereas the contralateral hippocampus rotated away from the vertical direction.SignificanceWe demonstrate that assessment of TBI‐induced hippocampal deformation by clinically translatable MRI methodologies detects subjects with prior TBI as well as those at high risk of PTE, paving the way toward subject stratification for antiepileptogenesis studies.
Mesenchymal stem cell therapy for focal epilepsy: A systematic review of preclinical models and clinical studies
Authors: Ramos‐Fresnedo Andres , Perez‐Vega Carlos , Domingo Ricardo A. , Lee Seung Jin , Perkerson Ralph B. , Zubair Abba C. , Takahisa Kanekiyo , Tatum William , Quinones‐Hinojosa Alfredo , Middlebrooks Erik. H. , Grewal Sanjeet S. 
Published: April 2022

AbstractDrug‐resistant epilepsy (DRE) is characterized by recurrent seizures despite appropriate treatment with antiseizure medication (ASM). Due to their regenerative and immunomodulatory potential, therapies with biologics such as mesenchymal stem cells (MSCs) offer a potential therapeutic benefit for structural causes of epilepsy, such as hippocampal sclerosis. In this article, we report a systematic review of the literature evaluating the preclinical and clinical studies of MSCs for DRE. Medline, Ovid EMBASE, Scopus, and the Cochrane Databases were searched electronically from their dates of inception to November 2021 using the following keywords: ((“mesenchymal”) AND (“stem cell”)) AND ((“epilepsy”) OR (“convulsion”) OR (“seizures”)). This review followed Preferred Reporting Items for Systemic Reviews and Meta‐Analyses (PRISMA) guidelines. The initial query identified 488 studies representing 323 unique manuscripts. After application of selection criteria, 15 studies were included in this systematic review; 11 were preclinical studies and 4 were clinical studies. All preclinical studies were performed in rodents and all clinical studies were phase 1 trials. Thus far, therapy with MSCs appears to be safe for use in humans, as no severe adverse events related directly to the therapy were reported. Furthermore, MSC therapy appears to provide a statistically significant clinical benefit by reducing the seizure burden of patients, reducing the electrophysiological biomarkers of epilepsy, and improving their comorbidities, such as depression and anxiety. In addition, animal studies reveal that the therapy exerts its effect by reducing aberrant mossy fiber sprouting (reduce excitatory pathways) and increasing γ‐aminobutyric acid (GABA)ergic interneurons (increase inhibitory pathways). Both preclinical and clinical studies have shown MSC therapy to be safe and preliminary effective, thus warranting further studies to investigate its therapeutic potential.
Field Testing of a Novel Drilling Technique to Expand Well Diameters at Depth in Unconsolidated Formations
Authors: van der Schans Martin L. , Bloemendal Martin , Robat Niels , Oosterhof Ate , Stuyfzand Pieter J. , Hartog Niels 
Published: April 2022

AbstractLarger well diameters allow higher groundwater abstraction rates. But particularly for the construction of wells at greater depth, it may be more cost‐efficient to only expand the borehole in the target aquifer. However, current drilling techniques for unconsolidated formations are limited by their expansion factors (<2) and diameters (<1000 mm). Therefore, we developed a new technique aiming to expand borehole diameters at depth in a controlled manner using a low‐pressure water jet perpendicular to the drilling direction and extendable by means of a pivoting arm. During a first field test, the borehole diameter was expanded 2.6‐fold from 600 to 1570 mm at a depth of 53.5 to 68 m and equipped with a well screen to create an expanded diameter gravel well (EDGW). In keeping with the larger diameter, the volume flux per m screen length was two times higher than conventional 860 mm diameter wells at the site in the subsequent 3 year production period. Although borehole clogging was slower on a volumetric basis and similar when normalized to borehole wall area, rehabilitation of particle clogging at the borehole wall was more challenging due to the thickness of the gravel pack. While jetting the entire borehole wall before backfilling holds promise to remove filter cake and thus limit particle clogging, we found that a second borehole (expanded 4.1‐fold to 2460 mm) collapsed during jetting. Overall, the EDGW technique has potential to make the use of deeper unconsolidated aquifers economically (more) feasible, although further understanding of the borehole stability and rehabilitation is required to assess its wider applicability.
The quality of life and comfort levels of hemodialysis patients with constipation: A descriptive and cross‐sectional study
Authors: Bulbul Elif , Cepken Tugba , Caliskan Ferda , Palabiyik Birgul , Sayan Canan , Kazancioglu Rumeyza 
Published: April 2022

AbstractIntroductionThis study was conducted to determine the constipation‐related quality of life, bowel habits, and comfort levels of constipated hemodialysis patients. It is known that the prevalence of constipation is high in hemodialysis patients.MethodsThis was a cross‐sectional and correlational study. The study included 164 hemodialysis patients who were determined to have constipation by examining the constipation statuses of 385 patients based on the Rome‐IV criteria. The study was reported according to the STrengthening the Reporting of OBservational studies in Epidemiology Declaration.FindingsIt was determined that as the constipation‐related quality of life of the patients increased, their comfort levels also increased. Constipation‐related quality of life increased in parallel with an increasing Bristol Stool Scale (BSS) score and an increasing number of bowel movements. The BSS scores of the patients were found to have a significant positive correlation with the patients' bowel movement frequencies and a significant negative correlation with their constipation frequencies. There was a negative correlation between years of dialysis and bowel movement frequencies. The presence of a previous gastrointestinal system complaint, the presence of an impact of constipation on the patient's dialysis session, and comfort levels were determined to be significant predictors of constipation‐related quality of life that explained 26.3% of the total variance in constipation‐related quality of life.DiscussionIn patients receiving hemodialysis treatment, constipation is an important and frequently encountered problem. Constipation leads to a reduction in quality of life and hemodialysis‐related comfort.
Child‐rearing experiences of female nurses working three shifts in South Korea: A qualitative study
Authors: Yoo Hye Jin , Shim JaeLan 
Published: April 2022

AbstractAimTo explore the experience of balancing a career with raising school‐aged children and working three shifts and to suggest working environment changes to balance these rolesBackgroundFemale nurses working three‐day shift rotations experience irregular life patterns, parenting conflicts, and confusion over nursing roles.IntroductionThis qualitative study examined nurses with early school‐aged children who work three‐day shift rotations in South Korea.MethodsA descriptive phenomenological approach employed in‐depth interviews to collect data from 12 married, female nurses with children aged 4–9 years. Data were analyzed using Colaizzi's phenomenological method, and reporting rigor was demonstrated using the Consolidated Criteria for Reporting Qualitative Research checklist.FindingsFour themes emerged in our findings: (1) challenging phase: facing a great crisis that completely shatters the axis of life; (2) chaos phase: endless inner conflict between work and parenting; (3) solutions for coping phase: struggling to continue a nursing career; and (4) expanding phase: stepping into the expanded world of care.ConclusionNurses working three‐day shift rotations try to balance their lives through interactions between nursing and child‐rearing. Further research is needed to create an environment that promotes this balance.Implications for nursing practice and policyTo provide a better work–life balance, policymakers should foster a participatory and supportive management style, ensure adequate nurse staffing, implement a flexible work system, provide clinical career opportunities, encourage professional autonomy and responsibility, and promote in‐service and shared social information or continuing education.
Identification of optimal reference genes for gene expression studies in a focal cerebral ischaemia model—Spatiotemporal effects
Authors: Pomierny Bartosz , Krzyzanowska Weronika , Jurczyk Jakub , Strach Beata , Skorkowska Alicja , Leonovich Innesa , Budziszewska Bogusława , Pera Joanna 
Published: April 2022

AbstractA proper reference gene (RG) is required to reliably measure mRNA levels in biological samples via quantitative reverse transcription PCR (RT‐qPCR). Various experimental paradigms require specific and stable RGs. In studies using rodent models of brain ischaemia, a variety of genes, such as β‐actin (Actb), hypoxanthine phosphoribosyltransferase 1 (Hprt1), peptidyl‐propyl isomerase A (Ppia) and glyceraldehyde‐3‐phosphate dehydrogenase (Gapdh), are used as RGs. However, most of these genes have not been validated in specific experimental settings. The aim of this study was to evaluate the time‐ and brain region‐dependent expression of RG candidates in a rat model of transient middle cerebral artery occlusion (tMCAO). The following genes were selected: Actb, Hprt1, Ppia, Gapdh, tyrosine 3‐monooxygenase/tryptophan 5‐monooxygenase activation protein, zeta (Ywhaz) and beta‐2 microglobulin (B2m). Focal cerebral ischaemia was induced by 90 min of tMCAO in male Sprague‐Dawley rats. Expression was investigated at four time points (12 and 24 h; 3 and 7 days) and in three brain areas (the frontal cortex, hippocampus and dorsal striatum) within the ischaemic brain hemisphere. The RT‐qPCR results were analysed using variance analysis and the ΔCt, GeNorm, NormFinder and BestKeeper methods. Data from these algorithms were ranked using the geometric mean of ranks of each analysis. Ppia, Hprt1 and Ywhaz were the most stable genes across the analysed brain areas and time points. B2m and Actb exhibited the greatest fluctuations, and the results for Gapdh were ambiguous.
Does hyaluronidase injected in periocular area change skin ultrastructure?: Standardized in vitro analysis
Authors: Aguilar‐González Marina , Davó‐Cabrera Juan , Rausell‐Fontestad Nuria , Botella‐Estrada Rafael , España‐Gregori Enrique , Pérez‐López Marta 
Published: April 2022

AbstractBackgroundTreatment with hyaluronic acid (HA) fillers as a strategy for rejuvenation has experienced a significant growth in recent years, accompanied by a parallel increase in its complications, the treatment of which, such as hyaluronidase, we must be aware of.Patients/Methods14 patients (28 eyes) had indication for upper blepharoplasty surgery in the Hospital Universitario y Politécnico La Fe. After surgery, periocular skin of one eye of each patient was infiltrated with 300 U of hyaluronidase (14 cases) while the skin of the fellow eye was preserved untreated (14 controls). All samples were studied by the Pathology department, and finally, 6 variables (skin structure alteration, degeneration of elastic fibers, deposits, collagen fibers destructuring, inflammation, and other findings) were analyzed.ResultsNo differences in skin structure, elastic fibers, and collagen dermal fibers were found between hyaluronidase‐treated skin and controls. A significant association between ex vivo application of hyaluronidase in periocular skin and the presence of amorphous extracellular deposits within the dermis was found.ConclusionsHyaluronidase applied ex vivo to periocular skin led to presence of deposits within the extracellular matrix compared to control eyelid skin but elastin and collagen dermis structure remained unaltered.