bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comprehensive toxicological analysis of trans-fatty acids (TFAs): the pro-apoptotic action of industrial TFAs is counteracted by polyunsaturated fatty acids
Authors: Hirata Yusuke , Nada Yuki , Inoue Aya , Sato Emiko , Noguchi Takuya , Matsuzawa Atsushi 
Published: September 2022

Abstract
trans-Fatty acids (TFAs) are unsaturated fatty acids containing at least one carbon-carbon double bond in trans configuration, which are classified into two groups according to their food source: industrial TFAs (iTFAs) and ruminant TFAs (rTFAs). Previous epidemiological evidence has demonstrated a preferential association of iTFAs, rather than rTFAs, with various diseases including cardiovascular diseases. However, it is still unknown how iTFA exerts its specific toxicity and what effective treatments are available to mitigate its toxicity. Here, we performed a comprehensive toxicological assessment of TFAs based on the toxicity mechanism that we established previously. We found that iTFAs including elaidic acid (EA), but not other types of fatty acids including rTFAs, had a strong pro-apoptotic effect upon treatment of extracellular ATP, a damage-associated molecular pattern that induces apoptosis through the apoptosis signal-regulating kinase 1 (ASK1)-p38 MAP kinase pathway. We also found that polyunsaturated fatty acids (PUFAs), such as docosahexaenoic acid (DHA), potently suppressed EA-dependent increase in ASK1 activation and apoptosis. These results demonstrate that iTFAs specifically exert toxicity by targeting ASK1, and that PUFAs serve as their effective suppressor. Our study provides a molecular basis for risk assessment of foods, and for new prevention and treatment strategies for TFA-related diseases.
Increased expression of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 is required for proliferation of mouse embryonic stem cells that are undergoing differentiation
Authors: Guzel Saime , Gurpinar Yunus , Altunok Tugba Hazal , Yalcin Abdullah 
Published: September 2022

Abstract
The unlimited proliferation capacity of embryonic stem cells (ESCs) coupled with their capability to differentiate into several cell types makes them an attractive candidate for studying the molecular mechanisms regulating self renewal and transition from pluripotent state. Although the roles of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase family (PFKFB1-4) in cell survival, proliferation, and differentiation in tumor cells have been studied, their role in mESCs biology is currently unkown. In the current study, Pfkfb isozyme expressions were analyzed in undifferentiated R1 and J1 mouse embryonic stem cells (mESCs) that were cultured in the presence and absence of leukemia inhibitory factor (LIF). We report that expression of the Pfkfb3 isoenzyme was markedly increased when mESCs were promoted to differentiate with LIF removal. We then demonstrated that Pfkfb3 silencing induced the differentiation marker Brachyury suggesting that Pfkfb3 may be required for the regulation of mesodermal differentiation of mESCs. Furthermore, we show that the increase in Pfkfb3 expression is required for the proliferation of early differentiated mESCs. Although these results provide important insights into the early differentiation of mESCs with regard to Pfkfb expressions, further mechanistic studies will be needed for understanding the pathways and mechanisms involved in regulation of proliferation and early differentiation of mESCs through Pfkfb3.
Angle Based Critical Nodes Detection (ABCND) for Reliable Industrial Wireless Sensor Networks
Authors: Shukla Shailendra 
Published: September 2022

Abstract
Node failure in the Wireless Sensor Networks (WSN) topology may lead to economic loss, endanger people, and cause environmental damage. It is found that reliability and security are the two most essential concerns in IWSN. Reliability can be achieved by adequately managing network topology using the structural approaches, where the critical wireless sensor nodes are precisely detected and protected. In this paper, we address the problem of critical node detection and present two-phase algorithms. In Phase-I, a 2D critical node (C-N) detection algorithm is proposed, which uses only RSSI information of the neighbor. This algorithm is also extended to work in 3D topology. In Phase II, a correlation-based reliable approach is proposed to increase the node resilience against node failure to achieve low overhead. The proposed algorithms (ABCND) requires mathcalO (log (N)) time for convergence and mathcalO (delta (log N)) for critical node detection, N represents the number of IoT devices, and delta is the cost required to forward and backward message. We compare our algorithm with the current state-of-the-art on C-N detection algorithms. The simulation results show that the proposed ABCND consumes 50% less energy to detect C-N and 90% to 95% reliable C-N.
Disturbed chaotic system synchronization via nonlinear robust interval state observers
Authors: KHEMIRI Wissal , TLILI Ali Sghaier 
Published: September 2022

Abstract
In this paper, a nonlinear interval state observer design method is synthesized for chaos synchronization between master (drive) and slave (response) systems based on the Luenberger-type observer. The stability study, analyzed with Lyapunov’s approach, is formulated in terms of linear matrix inequalities (LMIs) and subsequently to calculate the observation gain matrix. The stability of the designed state observer is analyzed within the Lyapunov approach and the LMI paradigm. Moreover, the nonlinear interval observer, comprising the lower and upper bound state observers, is used for the state reconstruction of nonlinear perturbed chaotic sys- tems subject to unknown but bounded external disturbances. The effi- ciency and high performances of the aforementioned state observer-based synchronization technique are demonstrated by a large study of numeri- cal simulation for the state synchronization of perturbed unified chaotic systems.
keywords : State synchronization Nonlinear perturbed chaotic sys- tems Nonlinear interval observer Lyapunov approach LMI paradigm
Rare variation: the absence of both the celiac trunk artery and the common hepatic artery
Authors: Zhang Zhenxing , Wang Shan , Ye Minfeng , Tao Feng , Guo Danling 
Published: September 2022

Abstract
Background
Knowledge of celiac trunk anatomy is important in gastrointestinal surgery, hepatopancreatobiliary surgery, transplantation and interventional radiology. Variations in the celiac trunk are common and should be predicted prior to these interventions.
Methods
A 58-years-old woman was admitted to our department for surgical treatment of gastric cancer (GC) confirmed by gastroduodenoscopy and gastric antrum biopsy. In the contrast-enhanced computed tomography (CT), we found an absence of both the celiac trunk artery (CA) and the common hepatic artery (CHA). Therefore, we used computerized three-dimensional (3D) vascular reconstruction technology to reconstruct the abdominal trunk and its branch vessels before operation.
Results
Computerized 3D vascular reconstruction confirmed an extremely rare vascular anomaly: the absence of both CA and CHA. The splenic artery (SA) and gastroduodenal artery (GDA) originated from the abdominal aorta (AA). The left gastric artery (LGA) originated from the AA directly above the junction of SA and the GDA. The left hepatic artery (LHA) originated from the left gastric artery (LGA). The right hepatic artery (RHA) originated from the superior mesenteric artery (SMA). Laparoscopic radical resection of GC was performed. This anomaly was also confirmed intraoperatively. This patient was discharged on the 10th day after surgery without any postoperative complication. There were no signs of tumor recurrence during the 6-month follow-up.
Conclusion
Correct identification of abnormal abdominal large blood vessels and their relationship with tumors before surgery is of great significance to avoid intraoperative blood vessel damage, major postoperative complications and the missing of lymph node dissection.
Variation in Heat Shock Protein 40kDa relates to divergence in thermotolerance among cryptic rotifer species
Authors: Kiemel Katrin , Gurke Marie , Paraskevopoulou Sofia , Havenstein Katja , Weithoff Guntram , Tiedemann Ralph 
Published: September 2022

AbstractGenetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such patterns. Here, we used the recently resolvedBrachionus calyciflorusspecies complex to investigate whether previously observed between species differences in thermotolerance and gene expression are also reflected in their genomic footprint. We identified a Heat Shock Protein gene (HSP 40kDa) which exhibits cross species pronounced structural variation. This gene exhibits species-specific fixed sites, alleles, and sites putatively under positive selection. These sites are located in protein binding regions involved in chaperoning and may therefore reflect adaptive diversification. By comparing three genetic markers (ITS, COI, HSP 40kDa), we revealed hybridization events between the cryptic species. The low frequency of introgressive haplotypes/alleles suggest a tight, but not fully impermeable boundary between the cryptic species.
Theoretical 2D protein gel of Helicobacter pylori
Authors: Ng Wenfa 
Published: September 2022

Abstract
Two-dimensional (2D) gel electrophoresis affords us a qualitative view of the proteome of an organism, which offers a rich understanding of the molecular weight and isoelectric points of proteins expressed under given environmental and nutritional conditions. But, clustering of proteins of similar molecular weight or isoelectric point or both makes separation of proteins difficult. To this end, a theoretical 2D protein gel calculated based on experimental proteome of an organism would provide important clues to the ability of 2D gel electrophoresis to separate two target proteins. More importantly, a theoretical 2D gel is a visualisation of the distribution of isoelectric points and molecular weights of all proteins in the species, which is important to gaining an approximate but high-level understanding of the physiological properties of the organism. This work presents the theoretical 2D protein gel of Helicobacter pylori.
Updates to the SUMER Spectral Atlas
Authors: Curdt Werner , wilhelm Klaus , Schühle Udo , Vial Jean-Claude , Lemaire Philippe , Bocchialini Karine 
Published: September 2022

Abstract
We present a re-worked version of the SUMER spectral atlas (2001). New identifications are given for more than100 emission lines, entries were corrected or added to the list.The solar emission curve - spectral radiance versus wavelength - in the SUMER spectral range is now available in digital format as electronic supplementary material. In order to reveal the true solaremission, second order photons from the Lyman continuum have been discarded near the long wavelength section in first order of diffraction.
Galacto-oligosaccharides increase abundance of beneficial probiotic bacteria, improve gut architecture and goblet cell expression in poorly performing piglets
Authors: Lee Adam , Mansbridge Stephen C. , Liang Lu , Connerton Ian F. , Mellits Kenneth H. 
Published: September 2022

Abstract

Background:Suckling is a unique period in porcine development where establishment of the microbiota is essential for future growth and health of animals. However, poor pre-weaning performance and failure to thrive without signs of clinical disease during lactation is of concern in pig production and incurs significant economic loss. Prebiotic galacto-oligosaccharides are functionally similar to those of mammalian milk and modulate gut architecture and intestinal microbiota in healthy pigs. Study objectives were to investigate the effects of complete milk replacer supplemented with 1 % (w/w) galacto-oligosaccharides on the microbiome, gut architecture and immunomodulatory goblet cell expression of poorly performing piglets that could benefit from milk replacement feeding when separated from sows and fit siblings in environmentally controlled pens. This was compared with piglets that received complete milk replacer without galacto-oligosaccharide supplementation. Production data over 21 days were collected in four separate and repeated feed trials beforepost-mortemduodenal, jejunal, ileal, caecal, colonic and rectal samples were collected from thirty-five piglets across the four trials and the microbiome composition assessed by 16s rRNA gene sequencing. Gut architectural features, villus crypt ratio and enumeration of goblet cells in tissues were assessed by histopathological techniques.Results:Milk replacer supplemented with galacto-oligosaccharides was palatable and well tolerated but had no significant effect upon performance or alpha-diversity. Galacto-oligosaccharides added to milk replacer significantly modulated microbial communities as demonstrated by beta-diversity and significantly increasedLactobacillusandBifidobacteriumthroughout the gastrointestinal tract. The most abundant taxa identified at genus level wereLactobacillus,Streptococcus,Prevotella,LactococcusandLeuconostoc. Milk replacer plus galacto-oligosaccharide fed piglets showed significantly improved gut architectural features and villus crypt ratio throughout the gastrointestinal tract as well as an increased number of barrier enhancing and immunomodulatory goblet cells.Conclusions:Galacto-oligosaccharide supplemented milk replacer significantly increased differential abundance of beneficial probiotic bacteria, particularlyLactobacillusandBifidobacterium,improved gut architecture and goblet cell expression in poorly performing piglets. In these respects, galacto-oligosaccharide supplemented milk replacer may be a useful addition to animal husbandry in poorly performing, non-thriving animals when moved to environmentally controlled pens away from sows and fit siblings, thereby modulating the microbiome and gastrointestinal tract performance.
Inhibition of Pathological Myofibroblast Differentiation of Valvular Interstitial Cell by Atorvastatin, Olmesartan, and Resveratrol in Experimental Heart Valve Model “Contemporary Model with Rabbit Valve”
Authors: Suwanto Denny , Lefi Achmad , Dharmadjati Budi Baktijasa , Oktaviono Yudi Her , Subagjo Agus , Sativa Oryza , Rachmi Dita Aulia , Nugraha Ricardo Adrian 
Published: September 2022

Abstract
Introduction. Recent studies revealed that differentiation of valvular interstitial cell into myoﬁbroblasts played an important role in pathological valve remodeling in rheumatic valvular disease.
Objective. To investigate effects of atorvastatin, olmesartan, and resveratrol on Transforming Growth Factor β1-induced fibrosis.
Methods. Valvular interstitial cell was isolated from 12-weeks male New Zealand rabbit (Oryctolagus cuniculus). Culture cells was divided into 4 groups, control group, group I (0.5 mg/mL Atorvastatin), group II (100 nmol/L Olmesartan), group III (50 μM/L Resveratrol) and group IV (combinations). All group were exposed to 100 nM Transforming Growth Factor β1 for 24 hours.
Results. Immunochemical staining demonstrated that cells were completely differentiated into myofibroblasts with mean expression of α-smooth muscle actin 24522.64±4566.994. Atorvastatin, olmesartan, resveratrol, and its combination significantly reduced α-smooth muscle actin expression (6823±1735.3, 6942.7±2455.9, 14176.2±3343.3, 5051.8±1612.2 respectively (p<0.001).
Conclusion. Our data showed atorvastatin, olmesartan, resveratrol, and its combination significantly reduce Transforming Growth Factor β1-induced valvular fibrosis.
Endorsement of N95 Respirators on Twitter: is correct, complete advice being given?
Authors: Ahmed Wasim , Khan M. Laeeq , Malik Aqdas , Krishnan Satish , Katz Matthew S 
Published: September 2022

Abstract
Research Objective
This study aimed to examine influential voices recommending N95 respirators during a peak in cases and deaths. Our study sought to understand whether influencers were providing accurate details about the correct type of N95 that would protect the wearer and those around them and the vital need to ensure a proper fit for the N95.
Study Design
Our study drew upon primary data from Twitter and retrieved n = 251,740 tweets from January 2021, which were filtered and analysed using social network analysis. The study drew upon mixed methods. Betweenness centrality was used to identify influential users recommending N95 face masks. A coding frame was developed to determine whether complete advice was provided. The tweets received as replies to each of the influencers were analysed using content analysis.
Principal Findings
Our study found that the most influential users recommending about N95 face masks were medical professionals and public health experts. Moreover, our data revealed that most tweets by health influencers recommending N95s did not provide further details about the correct type of N95 mask and the vital need to ensure a proper fit for the mask.
Implications for Policy and Practice
Healthcare influencers can play a vital role in disseminating accurate and timely information. Our study confirmed that prominent health influencers were not providing enough information about N95s. We produce three key recommendations for healthcare influencers. Our results are likely to be of interest to health services and for improving patient safety.
Monitoring and Evaluation of Gully Erosion in China's Largest Loess Tableland Based on SBAS-InSAR
Authors: Tian Haibo , Tao Yuxiang , Kou Pinglang , Alonso Andres , Luo Xiaobo , Gong Chenyu , Fan Yunpeng , Lei Changjian , Gou Yongcheng 
Published: September 2022

Abstract
Gully erosion is widespread in central China's ecologically fragile loess plateau. However, research on the monitoring and evaluation of large-scale fine erosion is scarce. Here, we use the sentinel-1A imagery during a rainy season, using the small baseline subset synthetic aperture radar interferometry (SBAS-InSAR) technology to calculate gully erosion for the largest loess plateau in China, the Dongzhiyuan tableland. The results showed that the average and cumulative subsidence rates of the whole study area are 9 mm/year and 6 mm/year respectively, while the subsidence within the gullies reached 160 mm/year and 70 mm/year, which was mainly caused by rainfall and landslides formed by groundwater softening slopes. In addition, erosion was more severe at the bottom of the gully closer to the lower reaches of the watershed, which may be related to the concentrated runoff downstream during rainfall. Interestingly, the erosion deformation was more intense on slopes 20m above the gully bottom caused by groundwater seepage. The results demonstrated the reliable capability of the SBAS-InSAR method in accurately assessing large-scale erosion deformation.
Evaluating a Community Engagement Model for Malaria Elimination in Haiti: Lessons from the Community Health Council (CHC) project (2019-2021)
Authors: Bardosh Kevin , Desir Luccene , Jean Lorence , Yoss Sarah , Poovey Brianna , Nute Andrew , de Rochars Madsen Valerie Beau , Telfort Marc-Aurèle , Benoit Fabiola , Noland Gregory S. 
Published: September 2022

Abstract
Background: Community engagement (CE) plays a critical part in malaria control and elimination efforts. However models of CE for malaria vary substantially, with more participatory approaches requiring higher levels of adaptive management in program design and implementation. In this paper, we evaluate the effectiveness of a volunteer-based CE model developed in Haiti in 2018. This model facilitated local leaders to organize and implement monthly anti-malaria activities in their communities and was implemented as part of the Malaria Zero consortium activities.
Methods: This program evaluation draw on quantitative and qualitative data collected from 23 Community Health Councils (CHCs) over a two-year period (2019-2021) in Grand’Anse department, a malaria hotspot region in Haiti.
Results: Monthly monitoring data showed that 100% of the 23 CHCs maintained functioning over the two-year period, with an average of 0.90 monthly meetings held with an 85% attendance rate. A high degree of transparency and diversity in membership helped create strong planning and involvement from members. CHCs conducted an average of 1.59 community-based activities per month, directly engaging an average of 123 people/month, with high levels of fluctuation indicative of local ownership and self-organization. This included school and church sensitization, environmental sanitation campaigns, mass education, support for case referrals and community mobilization during mass drug administration (MDA) and indoor residual spraying (IRS) campaigns. Members drew on the tradition of konbit (mutual self-help), local histories of health and development campaigns and a lexicon of “solidarity” in difficult times as they negotiated their agency as community volunteers. Small incentives played both symbolic and supportive roles and some level of politicization was viewed as inevitable, even beneficial. Rumours about financial and political profiteering of CHC volunteers took time to dispel while the tendency towards vertical planning in malaria control created conditions that excluded CHCs from some activities and generated resentment from members who felt sidelined by the government malaria program.
Conclusion: The CHC model was effective in promoting group solidarity and community-based anti-malaria activities over a two-year period in Haiti. With the end of the Malaria Zero consortium in early 2021, there is now an opportunity to better integrate this model into the primary healthcare system, evaluate the impact of the CHCs on malaria epidemiology, and promote the greater integration of CHCs with active surveillance and response activities.
Investigation of Mechanical and Printing Properties of Poly(Lactıic Acid) and Composite Filaments Used in 3d Printers
Authors: Şahin Gökçen , Özyıldırım Hasan , Şahin Anıl 
Published: September 2022

Abstract
In this study, it was investigated how the mechanical and printing properties of poly(lactic acid) (PLA) and composite filaments, which are increasingly used in 3D printers, change in a humid environment. PLA filament, PLA/Graphite and PLA/Polyhydroxybutyrate (PHB) composite filaments were used in the experiments. The filaments were exposed to 80% relative humidity for 15 days, then standard dog-bone specimens were printed from these filaments. Tensile test was applied to filaments and printed specimens, Fourier transform infrared spectrometry (FTIR) analyzes were made, scanning electron microscope (SEM) images of specimens and fracture surfaces were obtained. When the experiments were evaluated, it was observed that the print quality and tensile strength of 3D prints made from PLA, PLA/Graphite composite filaments exposed to humid environment decreased, while the print quality and tensile strength of PLA/PHB composite filament increased.