bluuebunny/crossref_metadata_2025_split huggingface dataset:
Obesity Increases Risk for Surgical Site Infections and Wound Dehiscence after Pediatric Foot Surgery: A Retrospective Cohort Review using the NSQIP-Pediatric Database
Authors: Zide Jacob R. , Farahani Farzam , Rodriguez Joel , Wukich Dane K. , Riccio Anthony 
Published: January 2022

Category: Other Introduction/Purpose: Both the incidence of childhood obesity and the number of obese children undergoing surgical procedures are increasing in pediatrics. As such, pediatric orthopaedic surgeons will likely encounter obese patients more frequently in their practice and a better understanding of the unique risks associated with obesity is valuable to maximize patient safety. The purpose of this study is, therefore, to retrospectively evaluate the relationship between obesity and post-operative outcomes in pediatric orthopaedic surgery patients across multiple institutions using a large national database. Methods: Pediatric patients who had undergone foot surgery were retrospectively identified by cross-referencing reconstructive foot-specific CPT codes with ICD-9/ICD-10 diagnosis codes using the American College of Surgeons 2012-2017 Pediatric National Surgical Quality Improvement (ACS-NSQIP-Pediatric) database. Patients were stratified into normal weight and obese cohorts based upon Center for Disease Control BMI-to-age growth charts. Patient demographics, comorbidities, intra-operative, and post- operative factors were compared between these two cohorts via univariate analysis with false discovery rate adjustment. Multivariable logistic regression models were then generated to assess for obesity as an independent predictor of post-operative complications. Results: Of the 3,924 patients identified, 1,063 (27.1%) were obese. Obese patients were more often male (64.7% vs 58.7%; p=0.001) and taller (56.3in vs 51.3in; p<0.001) than normal weight patients. There were no differences in pre-operative comorbidities between the two cohorts. Obese patients had a higher overall post-operative complication rate compared to normal weight patients (3.01% vs 1.32%; p=0.001). There was a significantly higher rate of wound dehiscence in obese patients (1.41% vs 0.59%; p=0.039) as well as a higher surgical site infection (SSI) rate that trended towards, but did not reach, statistical significance (1.32% vs. 0.59%; p=0.061). No differences were noted in unplanned readmissions (1.03% vs 0.9%; p=0.968) or unplanned reoperations (1.03% vs. 0.45%; p=0.175) within 30 days of surgery between the two groups. In multivariate analysis, obesity was found to be an independent predictor of both wound dehiscence (adjusted OR=2.16; 95%CI=1.05-4.50; p=0.037) and SSI (adjusted OR=3.03; 95%CI=1.39-6.61; p=0.005). Conclusion: Obese children undergoing foot surgery had higher overall complication rates than normal weight patients. Obese children undergoing foot surgery may be at higher risk for wound complications and surgical site infections than those of normal weight. This information may be useful in assessing and discussing surgical risks with patients and their families.
QTL mapping for growth-related traits by constructing the first genetic linkage map in Simao pine
Authors: Wang Dawei , Yang Lin , Shi Chen , Li Siguang , Tang Hongyan , He Chengzhong , Cai Nianhui , Duan Anan , Gong Hede 
Published: January 2022

AbstractBackgroundSimao pine is one of the primary economic tree species for resin and timber production in southwest China. The exploitation and utilization of Simao pine are constrained by the relatively lacking of genetic information. Construction a fine genetic linkage map and detecting quantitative trait locis (QTLs) for growth-related traits is a prerequisite section of Simao Pine's molecular breeding program.ResultsIn our study, a high-resolution Simao pine genetic map employed specific locus amplified fragment sequencing (SLAF-seq) technology and based on an F1pseudo-testcross population has been constructed. There were 11,544 SNPs assigned to 12 linkage groups (LGs), and the total length of the map was 2,062.85 cM with a mean distance of 0.37 cM between markers. According to the phenotypic variation analysis for three consecutive years, a total of seventeen QTLs for four traits were detected. Among 17 QTLs, there were six for plant height (Dh.16.1, Dh16.2, Dh17.1, Dh18.1–3), five for basal diameter (Dbd.17.1–5), four for needle length (Dnl17.1–3, Dnl18.1) and two for needle diameter (Dnd17.1 and Dnd18.1) respectively. These QTLs individually explained phenotypic variance from 11.0–16.3%, and the logarithm of odds (LOD) value ranged from 2.52 to 3.87.ConclusionsIn our study, a fine genetic map of Simao pine applied the technology of SLAF-seq has been constructed for the first time. Based on the map, a total of 17 QTLs for four growth-related traits were identified. It provides helpful information for genomic studies and marker-assisted selection (MAS) in Simao pine.
Study retention and attrition in a longitudinal cohort study including patient-reported outcomes, fieldwork and biobank samples: results of the Netherlands quality of life and Biomedical cohort study (NET-QUBIC) among 739 head and neck cancer patients and 262 informal caregivers
Authors: Jansen Femke , Brakenhoff Ruud H. , Baatenburg de Jong Rob J. , Langendijk Johannes A. , Leemans C. René , Takes Robert P. , Terhaard Chris H. J. , Smit Jan H. , Verdonck-de Leeuw Irma M. 
Published: January 2022

Abstract
Background
Longitudinal observational cohort studies in cancer patients are important to move research and clinical practice forward. Continued study participation (study retention) is of importance to maintain the statistical power of research and facilitate representativeness of study findings. This study aimed to investigate study retention and attrition (drop-out) and its associated sociodemographic and clinical factors among head and neck cancer (HNC) patients and informal caregivers included in the Netherlands Quality of Life and Biomedical Cohort Study (NET-QUBIC).

Methods
NET-QUBIC is a longitudinal cohort study among 739 HNC patients and 262 informal caregivers with collection of patient-reported outcome measures (PROMs), fieldwork data (interview, objective tests and medical examination) and biobank materials. Study retention and attrition was described from baseline (before treatment) up to 2-years follow-up (after treatment). Sociodemographic and clinical characteristics associated with retention in NET-QUBIC components at baseline (PROMs, fieldwork and biobank samples) and retention in general (participation in at least one component) were investigated using Chi-square, Fisher exact or independent t-tests (p< 0.05).

Results
Study retention at 2-years follow-up was 80% among patients alive (66% among all patients) and 70% among caregivers of patients who were alive and participating (52% among all caregivers). Attrition was most often caused by mortality, and logistic, physical, or psychological-related reasons. Tumor stage I/II, better physical performance and better (lower) comorbidity score were associated with participation in the PROMs component among patients. No factors associated with participation in the fieldwork component (patients), overall sample collection (patients and caregivers) or PROMs component (caregivers) were identified. A better performance and comorbidity score (among patients) and higher age (among caregivers) were associated with study retention at 2-years follow-up.

Conclusions
Retention rates were high at two years follow-up (i.e. 80% among HNC patients alive and 70% among informal caregivers with an active patient). Nevertheless, some selection was shown in terms of tumor stage, physical performance, comorbidity and age, which might limit representativeness of NET-QUBIC data and samples. To facilitate representativeness of study findings future cohort studies might benefit from oversampling specific subgroups, such as patients with poor clinical outcomes or higher comorbidity and younger caregivers.
Giant dorsal lipofibromatosis in an infant: a case report
Authors: Li Zhiyu , Zou Yuqing , Xu Guangqi , Bi Jianhai , Huo Ran 
Published: January 2022

Abstract
Background
Lipofibromatosis is a rare, benign, soft tissue tumor that usually presents in children. Low incidence and lack of specificity in clinical presentation make its diagnosis difficult.

Case presentation
This is a case report of a patient with a giant lipofibromatosis on the back that resembles an infantile hemangioma, which posed great difficulty in diagnosis due to atypical clinical manifestations. After the postoperative pathological and immunohistochemical examination and fluorescence in situ hybridization, the patient was finally diagnosed with lipofibromatosis.

Conclusions
The incidence of fibromatosis was low. This case presents an atypical clinical manifestation since the tumor growth was on the back, and this can easily cause misdiagnosis. This case suggests that the diagnosis of lipofibromatosis depends on the pathology and fluorescence in situ hybridization.
Genome-wide analysis of cell-Free DNA methylation profiling with MeDIP-seq identified potential biomarkers for colorectal cancer
Authors: Zhang Xin , Li Tao , Niu Qiang , Qin Chang-jiang , Zhang Ming , Wu Guang-ming , Li Hua-zhong , Li Yan , Wang Chen , Du Wen-fei , Wang Chen-yang , Zhao Qiang , Zhao Xiao-dong , Wang Xiao-liang , Zhu Jian-bin 
Published: January 2022

Abstract
Background
Colorectal cancer is the most common malignancy and the third leading cause of cancer-related death worldwide. This study aimed to identify potential diagnostic biomarkers for colorectal cancer by genome-wide plasma cell-free DNA (cfDNA) methylation analysis.

Methods
Peripheral blood from colorectal cancer patients and healthy controls was collected for cfDNA extraction. Genome-wide cfDNA methylation profiling, especially differential methylation profiling between colorectal cancer patients and healthy controls, was performed by methylated DNA immunoprecipitation coupled with high-throughput sequencing (MeDIP-seq). Logistic regression models were established, and the accuracy of this diagnostic model for colorectal cancer was verified using tissue-sourced data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) due to the lack of cfDNA methylation data in public datasets.

Results
Compared with the control group, 939 differentially methylated regions (DMRs) located in promoter regions were found in colorectal cancer patients; 16 of these DMRs were hypermethylated, and the remaining 923 were hypomethylated. In addition, these hypermethylated genes, mainly PRDM14, RALYL, ELMOD1, and TMEM132E, were validated and confirmed in colorectal cancer by using publicly available DNA methylation data.

Conclusions
MeDIP-seq can be used as an optimal approach for analyzing cfDNA methylomes, and 12 probes of four differentially methylated genes identified by MeDIP-seq (PRDM14, RALYL, ELMOD1, and TMEM132E) could serve as potential biomarkers for clinical application in patients with colorectal cancer.
Hybrid gastroenterostomy using a lumen-apposing metal stent: a case report focusing on misdeployment and systematic review of the current literature
Authors: Fabbri Carlo , Binda Cecilia , Fugazzola Paola , Sbrancia Monica , Tomasoni Matteo , Coluccio Chiara , Jung Carlo Felix Maria , Prosperi Enrico , Agnoletti Vanni , Ansaloni Luca 
Published: January 2022

Abstract
Background
Gastric outlet obstruction can result from several benign and malignant diseases, in particular gastric, duodenal or pancreatic tumors. Surgical gastroenterostomy and enteral endoscopic stenting have represented effective therapeutic options, although recently endoscopic ultrasound-guided gastroenterostomy using lumen-apposing metal stent (LAMS) is spreading improving the outcome of this condition. However, this procedure, although mini-invasive, is burdened with not negligible complications, including misdeployment.

Main body
We report the case of a 60-year-old male with gastric outlet obstruction who underwent ultrasound-guided gastroenterostomy using LAMS. The procedure was complicated by LAMS misdeployment being managed by laparoscopy-assisted placement of a second LAMS.
We performed a systematic review in order to identify all reported cases of misdeployment in EUS-GE and their management. The literature shows that misdeployment occurs in up to 10% of all EUS-GE procedures with a wide spectrum of possible strategies of treatment.

Conclusion
The here reported hybrid technique may offer an innovative strategy to manage LAMS misdeployment when this occurs. Moreover, a hybrid approach may be valuable to overcome this complication, especially in early phases of training of EUS-guided gastroenterostomy.
Application of Legendre polynomials based neural networks for the analysis of heat and mass transfer of a non-Newtonian fluid in a porous channel
Authors: Khan Naveed Ahmad , Sulaiman Muhammad , Kumam Poom , Alarfaj Fawaz Khaled 
Published: January 2022

AbstractIn this paper, the mathematical models for flow and heat-transfer analysis of a non-Newtonian fluid with axisymmetric channels and porous walls are analyzed. The governing equations of the problem are derived by using the basic concepts of continuity and momentum equations. Furthermore, artificial intelligence-based feedforward neural networks (ANNs) are utilized with hybridization of a generalized normal-distribution optimization (GNDO) algorithm and sequential quadratic programming (SQP) to study the heat-transfer equations and calculate the approximate solutions for the momentum of a non-Newtonian fluid. Legendre polynomials based Legendre neural networks (LNN) are used to develop a mathematical model for the governing equations, which are further exploited by the global search ability of GNDO and SQP for rapid localization convergence. The proposed technique is applied to study the effect of variations in Reynolds number Re on the velocity profile $(f^{\prime })$
(

f
′

)
and the temperature profile $(q)$
(
q
)
. The results obtained by the LeNN-GNDO-SQP algorithm are compared with the differential transformation method (DTM), which shows the stability of the results and the correctness of the technique. Extensive graphical and statistical analyses are conducted in terms of minimum, mean, and standard deviation based on fitness value, absolute errors, mean absolute deviation (MAD), error in the Nash–Sutcliffe efficiency (NSE), and root mean square error (RMSE).
Exome sequencing identifies a novel GUCY2D mutation in an Iranian family with Leber congenital amaurosis-1: a case report
Authors: Neissi Mostafa , Al-Badran Adnan Issa , Mohammadi-Asl Javad 
Published: January 2022

Abstract
Background
Leber congenital amaurosis (LCA), the severe form of inherited retinal degenerative disorder, is a prevalent disorder in the first year of life. Recently, genetic studies discovered that different gene mutations are responsible for LCA clinical manifestations.

Case presentation
In this study, we applied whole exome sequencing (WES) to identify probable gene defects in an Iranian girl with LCA-1. We found a novel disease-causing GUCY2D gene mutation (c.2348T > C; p.L783P), located in exon 12 (NM_000180), causing a missense mutation that has been changed the coding protein. The WES-identified variant was confirmed by Sanger sequencing for the patient and her healthy parents. Submitted to genetic counseling that the patient was 1-year old and blindness from birth.

Conclusions
Our findings establish that this detected GUCY2D-p.L783P mutation is the pathogenic variant for LCA-1. This is the first genetic study indicating that c.2348T > C missense mutation in the homozygous state in GUCY2D gene is responsible for the LCA-1 phenotype.
A hybrid TEG/evacuated tube solar collectors for electric power generation and space heating
Authors: Al-Tahaineh Hamza , AlEssa Abdullah H. M. 
Published: January 2022

AbstractThe present work aimed to examine the performance of a thermoelectric generator (TEG) augmented with a hydronic evacuated tube solar collector heat exchanger used to heat a cold zone. TEGs were operated on the temperature difference between hot water circulated through the heat exchanger and the cold temperature of the surrounding space. The setup model of a heat exchanger with TEGs installed on the outer surface was examined numerically under steady state conditions for natural and forced convection modes. The results obtained show that 1.03 W of electricity could be produced when the temperature differences across the TEG and air velocity are 60 °C and 0.5 m/s, respectively. Also, an increase of 17.47% of TEG power was achieved for each 5 °C drop in surrounding space temperature and by 11% for each 5 °C rise in circulated hot water temperature. Besides the importance of improving TEG efficiencies, the amount of generated electricity may be valuable when large-surface area heat exchanger units are installed in large systems.
Effects of carbon dioxide purities on mitotic index in lymphocyte culture and metaphase chromosome quality
Authors: Purnami Sofiati , Wibisari Indri Permata , Suvifan Viria Agesti , Nurhayati Siti , Ramadhani Dwi 
Published: January 2022

Abstract. Purnami S, Wibisari IP, Suvifan VA, Nurhayati S, Ramadhani D. 2021. Effects of carbon dioxide purities on mitotic index in lymphocyte culture and metaphase chromosome quality. Nusantara Bioscience 13: 171-176. The metaphase chromosome spread quality is necessary for a faster individual dose prediction following radiological accidents using dicentric chromosome assay. It is well known that the low-quality metaphase chromosome spreads can lead to false positives of dicentric chromosome identification. Thus, evaluation of the main variable that influences the preparation of high-quality metaphase chromosome spreads is important to perform. Until now, no studies have assessed the effects of CO2 purities on metaphase chromosome spread quality. This study aimed to evaluate the effects of carbon dioxide (CO2) purities on lymphocyte proliferation, and the quality of metaphase chromosome spreads to improve the chromosome aberration assay for cytogenetic biodosimetry purposes. Whole blood samples from three different subjects were cultured and incubated for 48 hours with two different grades of CO2 (high purity and food grades) and without CO2. The mitotic index (MI) from each subject was assessed, and the quality of metaphase chromosome spreads was evaluated by comparing the lengths of chromosomes 1, 2, and 21. Statistical analysis revealed that the difference between manual and automatic MI under three different conditions of CO2 purity was not statistically significant (p = 0.162; p = 0.901). Comparative analysis of the lengths of chromosomes 1, 2, and 21 from 145 metaphases also showed a difference that was not statistically significant (p = 0.745; p = 0.915; p = 0.399). Overall, our findings suggest that CO2 purities do not impair lymphocyte proliferation or metaphase quality. Further investigation should include other technical improvements such as drop-slide optimization.
Out-of-Plane Structural Performance of Compressed Earth Block Walls Subject to Quasistatic Loading
Authors: Saad Ahmad S. , Ahmed Taha A. , Yassin Mohammad H. , Radwan Ali I. , Ezzedine Aya I. 
Published: January 2022

Abstract
This study constitutes a step forward in the overall effort to evaluate the applicability of compressed earth blocks (CEBs) as an environmentally friendly construction alternative in Kuwait. The study is part of a series of experiments that started by assessing different soil samples from various regions in Kuwait. It was discovered that soils in the northern areas (near Al-Sabriyah district and Boubyan Island) contain a good mixture of clayey and silty deposits that can be used to produce CEBs. The collected soil from this region was used in producing CEBs. Three half-scaled walls were constructed and were laterally tested in the out-of-plane direction using quasistatic loading. One of these walls was strengthened using carbon fiber reinforced polymer (CFRP) laminates. A fourth wall made from regular concrete masonry units (CMU) was also tested and compared with the results from the CEB walls. The tests showed the latter had a significantly reduced lateral capacity compared with the wall made from CMUs. It emerged from the test results that a higher percentage of stabilizer in the CEB production enhances the performance of walls made from these CEB in their out-of-plane direction. This is especially the case in terms of stiffness, strength, displacements, and energy dissipated. Results revealed that strengthening CEB walls can alter their structural behavior from flexural dominated to shear dominated failure with an increase of 116 % in the wall strength (nearly doubled) under lateral loading in the out-of-plane direction.
Prevención, diagnóstico y tratamiento de la sepsis neonatal: Guía de práctica clínica basada en evidencias del Instituto Nacional Materno Perinatal del Perú
Authors: Dávila Aliaga Carmen , Hinojosa Pérez Rosemary , Mendoza Ibáñez Elina , Gómez Galiano Walter , Espinoza Vivas Ylia , Torres Marcos Elsa , Velásquez Vásquez Carlos , Ayque Rosas Fernando , Alvarado Zelada Jackelyne , Corcuera Segura Gloria , Beltrán Gallardo Nora , Gonzáles Castillo Josefa , Guevara Ríos Enrique , Huamán Sánchez Karen , Castillo Villacrez Celia , Reyes Puma Nora , Caballero Ñopo Patricia 
Published: January 2022

Introducción. La sepsis neonatal es un conjunto de signos y síntomas clínicos causados por una infección sistémica, asociada a factores de riesgo de tipo materno, neonatal u hospitalario. Objetivo. Brindar recomendaciones informadas por la mejor evidencia disponible para la prevención, diagnóstico y tratamiento de la sepsis neonatal. Métodos. Se desarrolló una guía de práctica clínica (GPC) basada en evidencias, mediante un proceso de adaptación, a cargo de un equipo de metodólogos y médicos neonatólogos expertos en el manejo clínico de la sepsis neonatal. Se realizó la búsqueda y preselección de GPC que respondan al alcance y objetivos planteados, utilizando el instrumento AGREE-II se evalúo la calidad metodológica de las guías para decidir su adaptación. Se realizó una búsqueda sistemática en múltiples bases de datos: Medline/PubMed, Embase/Ovid, Cochrane Library y LILACS, para identificar la evidencia que responda a las preguntas de la guía. Estas fueron seleccionadas y analizadas críticamente por pares clínicos y metodológicos, las recomendaciones fueron elaboradas usando el enfoque GRADE. Resultados. Se formularon 16 preguntas clínicas y recomendaciones basadas en evidencia a las que se llegó, mediante un diálogo deliberativo de expertos clínicos de diferentes hospitales de referencia para el manejo de la sepsis neonatal en el Perú. Las recomendaciones abordan la identificación de factores de riesgo, el uso de métodos confirmatorios y el tratamiento antibiótico como profilaxis y durante manejo de la enfermedad. Conclusiones: La GPC permite estandarizar el manejo clínico de la sepsis neonatal, así como la identificación de necesidades de investigación a realizarse en el contexto peruano.