bluuebunny/crossref_metadata_2025_split huggingface dataset:
High‐power DC‐DC converter with proposed HSFNA MPPT for photovoltaic based ultra‐fast charging system of electric vehicles
Authors: Priyadarshi Neeraj , Bhaskar M. S. , Sanjeevikumar P. , Azam Farooque , Khan Baseem 
Published: June 2022

AbstractThis research paper describes the implementation of a photovoltaic (PV) fed energy‐efficient high‐power DC‐DC converter for ultra‐fast charging systems with a proposed hybrid simplified Firefly and neighborhood attraction firefly (HSFNA) algorithm for maximum power point tracking (MPPT). The proposed high‐gain step‐up SEPIC converter is employed to deliver efficient MPPT operation, higher step‐up voltage gain, higher efficacy, and a more straightforward controlling mechanism. It provides continuous input current, maximum current handled ability, and higher quality DC voltage generation suitable for battery charging operation. Moreover, an isolated unidirectional dual‐bridge converter (IUDBC), DC‐DC converter, has been employed for ultra‐fast battery charging of electric vehicles. This accomplishes higher current charging to load, providing power transmission ability per battery demand by controlling several charging actions. Moreover, an ideal PV model approach has been implemented in this research work, providing accurate analysis of estimation of model parameters mathematically. Further, a proposed HSFNA based MPPT provides higher convergence velocity, fast‐tracking, accurate responses, higher efficiency, and can track global maximum power point (GMPP) under partial shade and varying operating conditions. Simulation and practical responses confirmed the effectiveness of the system design for ultra‐fast charging systems.
A new method to calculate corona losses for active conductors considering real transmission line unbalance
Authors: Llamo‐Laborí Héctor Silvio , Santos‐Fuentefria Ariel 
Published: June 2022

AbstractThis paper shows the characteristics of an algorithm that calculates the corona losses of transmission lines. The line can have up to four circuits, with any configuration, with single or bundled conductors, and taking into account the line unbalance. This is achieved by modelling the lines in phase quantities, for active conductor, and calculating the corona losses for each one. The results are given per active conductor and average per bundle. In lines with several conductors per phase the results are given per circuit and a total one. These characteristics allow choosing the most convenient configuration and spacing for the conductor bundles, which will reduce and balance corona losses. The results found with this method show that the value of the losses calculated with the formulas with transmission lines symmetrically transposed is conservative. The configuration and the number of conductors per phase have a noted influence in the value and the asymmetry of the corona losses. In double circuit lines, corona losses can be reduced by changing the arrangement of the phases of circuit two in reference to circuit one, with the advantage that, at the design stage, those changes are costless.
A general stability-driven approach for the refinement of multi-planet systems
Authors: Stalport M. , Delisle J.-B. , Udry S. , Matthews E. C. , Bourrier V. , Leleu A. 
Published: June 2022

Context. Over recent years, the number of detected multi-planet systems has grown significantly. An important subclass of these are the compact configurations. Precise knowledge of this subclass is crucial for understanding the conditions in which planetary systems form and evolve. However, observations often leave these systems with large uncertainties, notably on the orbital eccentricities. This is especially prominent for systems with low-mass planets detected with radial velocities, and increasing numbers of these are being discovered in the exoplanet population. Refining these parameters with the help of orbital stability arguments is becoming a common approach.
Aims. Such dynamical techniques can be computationally expensive. In this work, we use an alternative procedure that is orders of magnitude faster than classical N-body integration approaches, and has the potential to narrow down parameter uncertainties.
Methods. We coupled a reliable exploration of the parameter space with the precision of the Numerical Analysis of Fundamental Frequencies (Laskar, J. 1990, Icarus, 88, 266) fast chaos indicator. We also propose a general procedure to calibrate the NAFF indicator on any multi-planet system without additional computational cost. This calibration strategy is illustrated using the compact multiplanet system HD 45364, in addition to yet-unpublished measurements obtained with the HARPS and CORALIE high-resolution spectrographs. We validate the calibration approach by a comparison with long integrations performed on HD 202696. We test the performances of this stability-driven approach on two systems with different architectures: first we study HD 37124, a three-planet system composed of planets in the Jovian regime; then, we analyse the stability constraints on HD 215152, a compact system of four low-mass planets.
Results. We revise the planetary parameters for HD 45364, HD 202696, HD 37124, and HD 215152, and provide a comprehensive view of the dynamical state these systems are in.
Conclusions. We demonstrate the potential of the NAFF stability-driven approach to refine the orbital parameters and planetary masses. We stress the importance of undertaking systematic global dynamical analyses on every new multi-planet system discovered.
Metabolic and immune-sensitive contacts between lipid droplets and endoplasmic reticulum reconstituted in vitro
Authors: Kamerkar Sukrut , Singh Jagjeet , Tripathy Subham , Bhonsle Hemangi , Kumar Mukesh , Mallik Roop 
Published: June 2022

Coordinated cell function requires a variety of subcellular organelles to exchange proteins and lipids across physical contacts that are also referred to as membrane contact sites. Such organelle-to-organelle contacts also evoke interest because they can appear in response to metabolic changes, immune activation, and possibly other stimuli. The microscopic size and complex, crowded geometry of these contacts, however, makes them difficult to visualize, manipulate, and understand inside cells. To address this shortcoming, we deposited endoplasmic reticulum (ER)-enriched microsomes purified from rat liver or from cultured cells on a coverslip in the form of a proteinaceous planar membrane. We visualized real-time lipid and protein exchange across contacts that form between this ER-mimicking membrane and lipid droplets (LDs) purified from the liver of rat. The high-throughput imaging possible in this geometry reveals that in vitro LD–ER contacts increase dramatically when the metabolic state is changed by feeding the animal and also when the immune system is activated. Contact formation in both cases requires Rab18 GTPase and phosphatidic acid, thus revealing common molecular targets operative in two very different biological pathways. An optical trap is used to demonstrate physical tethering of individual LDs to the ER-mimicking membrane and to estimate the strength of this tether. These methodologies can potentially be adapted to understand and target abnormal contact formation between different cellular organelles in the context of neurological and metabolic disorders or pathogen infection.
Normative values for heart rate response to exercise in young athletes at 10–18 years old
Authors: Mascherini Gabriele , Galanti Giorgio , Stefani Laura , Izzicupo Pascal 
Published: June 2022

ABSTRACTThe assessment of the chronotropic response during acute physical exertion can allow the discovery of many cardiovascular diseases even at a young age. However, the increase in heart rate (HR) depends on the age and sex of the subject and the modality of the graded exercise test. This study aims to provide sex‐ and age‐related normative values for heart rate performance in young athletes aged 10–18. A retrospective study was carried out on 7896 young athletes (5356 males and 2540 females) aged between 10 and 18 who underwent pre‐participation screening to obtain eligibility for competitive sport. First, anthropometric parameters, performance data, and HR are reported. Thus, each age calculated third, tenth, twenty‐fifth, fiftieth, seventy‐fifth, ninetieth, and ninety‐seventh percentiles for the stage‐by‐stage HR response, according to sex and graded exercise test modality category. Young female athletes of all ages showed lower performance with fewer stages performed on the cycle ergometer and the treadmill. Young male athletes on treadmill and cycle ergometers show lower HR values at submaximal intensities. The treadmill allows a longer duration than the cycle ergometer for males and females. Sex, age, and the specificity of the movement performed must be considered in assessing the chronotropic response in the young population, particularly for those who carry out a training program. In addition, providing reference values of HR response to acute physical exertion may allow for a better functional assessment of the young athletes.
Late prenatal immune activation in mice induces transgenerational effects via the maternal and paternal lineages
Authors: Raymann Stephanie , Schalbetter Sina M , Schaer Ron , Bernhardt Alexandra C , Mueller Flavia S , Meyer Urs , Weber-Stadlbauer Ulrike 
Published: June 2022

AbstractPrenatal exposure to infectious or noninfectious immune activation is an environmental risk factor for neurodevelopmental disorders and mental illnesses. Recent research using animal models suggests that maternal immune activation (MIA) during early to middle stages of pregnancy can induce transgenerational effects on brain and behavior, likely via inducing stable epigenetic modifications across generations. Using a mouse model of viral-like MIA, which is based on gestational treatment with poly(I:C), the present study explored whether transgenerational effects can also emerge when MIA occurs in late pregnancy. Our findings demonstrate that the direct descendants born to poly(I:C)-treated mothers display deficits in temporal order memory, which are similarly present in second- and third-generation offspring. These transgenerational effects were mediated via both the maternal and paternal lineages and were accompanied by transient changes in maternal care. In addition to the cognitive effects, late prenatal immune activation induced generation-spanning effects on the prefrontal expression of gamma-aminobutyric acid (GABA)ergic genes, including parvalbumin and distinct alpha-subunits of the GABAA receptor. Together, our results suggest that MIA in late pregnancy has the potential to affect cognitive functions and prefrontal gene expression patterns in multiple generations, highlighting its role in shaping disease risk across generations.
Pretreatment Methods for the Determination of Antibiotics Residues in Food Samples and Detected by Liquid Chromatography Coupled with Mass Spectrometry Detectors: A Review
Authors: Li Fan , Luo Jinwen , Zhu Bingqi , Liu Zhu 
Published: June 2022

Abstract
With the increasing use of antibiotics worldwide, antibiotic monitoring has become a topic of concern. After metabolizing of antibiotics in animals, the metabolites enter the environment through excreta or ingested by the human body via food chain that may exacerbate the emergence of antibiotic resistance and then threaten human’s life. This article summarized several analytical methods used for the determination of antibiotics in recent 10 years. Due to the complex matrices and low concentration level of antibiotics in the food samples, a reliable analysis method is required to maximize the recovery rate. Several techniques like solid phase extraction (SPE), dispersive liquid–liquid microextraction (DLLME) and QuEChERS have been frequently used in the pretreatment process for analytes extraction and concentration. After the pretreatment, ultra-high performance liquid chromatography combined with mass spectrometry has been a reliable method for quantitative analysis and is able to determine multiple antibiotics simultaneously. This review also gives an overview about analytical conditions for antibiotics residues in different food samples and their method validation parameters.
Targeted Assessment of Mucosal Immune Gene Expression Predicts Clinical Outcomes in Children with Ulcerative Colitis
Authors: Clarkston Kathryn , Karns Rebekah , Jegga Anil G , Sharma Mihika , Fox Sejal , Ojo Babajide A , Minar Phillip , Walters Thomas D , Griffiths Anne M , Mack David R , Boyle Brendan , LeLeiko Neal S , Markowitz James , Rosh Joel R , Patel Ashish S , Shah Sapana , Baldassano Robert N , Pfefferkorn Marian , Sauer Cary , Kugathasan Subra , Haberman Yael , Hyams Jeffrey S , Denson Lee A , Rosen Michael J 
Published: June 2022

AbstractBackground and AimsWe aimed to determine whether a targeted gene expression panel could predict clinical outcomes in paediatric ulcerative colitis [UC] and investigated putative pathogenic roles of predictive genes.MethodsIn total, 313 rectal RNA samples from a cohort of newly diagnosed paediatric UC patients (PROTECT) were analysed by a real-time PCR microfluidic array for expression of type 1, 2 and 17 inflammation genes. Associations between expression and clinical outcomes were assessed by logistic regression. Identified prognostic markers were further analysed using existing RNA sequencing (RNA-seq) data sets and tissue immunostaining.ResultsIL13RA2 was associated with a lower likelihood of corticosteroid-free remission (CSFR) on mesalamine at week 52 (p = .002). A model including IL13RA2 and only baseline clinical parameters was as accurate as an established clinical model, which requires week 4 remission status. RORC was associated with a lower likelihood of colectomy by week 52. A model including RORC and PUCAI predicted colectomy by 52 weeks (area under the receiver operating characteristic curve 0.71). Bulk RNA-seq identified IL13RA2 and RORC as hub genes within UC outcome-associated expression networks related to extracellular matrix and innate immune response, and lipid metabolism and microvillus assembly, respectively. Adult UC single-cell RNA-seq data revealed IL13RA2 and RORC co-expressed genes were localized to inflammatory fibroblasts and undifferentiated epithelial cells, respectively, which was supported by protein immunostaining.ConclusionTargeted assessment of rectal mucosal immune gene expression predicts 52-week CSFR in treatment-naïve paediatric UC patients. Further exploration of IL-13Rɑ2 as a therapeutic target in UC and future studies of the epithelial-specific role of RORC in UC pathogenesis are warranted.
DENTIST—using long reads for closing assembly gaps at high accuracy
Authors: Ludwig Arne , Pippel Martin , Myers Gene , Hiller Michael 
Published: June 2022

Abstract

Background
Long sequencing reads allow increasing contiguity and completeness of fragmented, short-read–based genome assemblies by closing assembly gaps, ideally at high accuracy. While several gap-closing methods have been developed, these methods often close an assembly gap with sequence that does not accurately represent the true sequence.

Findings
Here, we present DENTIST, a sensitive, highly accurate, and automated pipeline method to close gaps in short-read assemblies with long error-prone reads. DENTIST comprehensively determines repetitive assembly regions to identify reliable and unambiguous alignments of long reads to the correct loci, integrates a consensus sequence computation step to obtain a high base accuracy for the inserted sequence, and validates the accuracy of closed gaps. Unlike previous benchmarks, we generated test assemblies that have gaps at the exact positions where real short-read assemblies have gaps. Generating such realistic benchmarks for Drosophila (134 Mb genome), Arabidopsis (119 Mb), hummingbird (1 Gb), and human (3 Gb) and using simulated or real PacBio continuous long reads, we show that DENTIST consistently achieves a substantially higher accuracy compared to previous methods, while having a similar sensitivity.

Conclusion
DENTIST provides an accurate approach to improve the contiguity and completeness of fragmented assemblies with long reads. DENTIST's source code including a Snakemake workflow, conda package, and Docker container is available at https://github.com/a-ludi/dentist. All test assemblies as a resource for future benchmarking are at https://bds.mpi-cbg.de/hillerlab/DENTIST/.
Role of MaABI5-like in abscisic acid-induced cold tolerance of ‘Fenjiao’ banana fruit
Authors: Song Zunyang , Lai Xiuhua , Chen Hangcong , Wang Lihua , Pang Xuequn , Hao Yanwei , Lu Wangjin , Chen Weixin , Zhu Xiaoyang , Li Xueping 
Published: June 2022

AbstractAbscisic acid (ABA) is a phytohormone essential for plants to respond to various environmental stresses, and abscisic acid-insensitive 5 (ABI5) is a basic leucine zipper transcription factor of the ABA signaling pathway. Exogenous ABA induces cold tolerance in bananas; however, the role of MaABI5-like in ABA-induced cold tolerance remains unexplored. The present study found that exogenous ABA alleviated chilling injury of ‘Fenjiao’ banana, induced the accumulation of endogenous ABA, unsaturated fatty acids, and flavonoid content, and reduced the saturated fatty acid content. Moreover, ABA treatment upregulated the transcription levels of MaABI5-like, fatty acid desaturation genes, and flavonoid synthesis-related genes during cold storage. More interestingly, MaABI5-like directly interacted with the promoter of genes related to fatty acid desaturation (MaFAD3-1, MaFAD3-4, MaFAD3-5, MaFAD6-2, MaFAD6-3) and flavonoid synthesis (MaPAL-like, MaPAL-like1, MaC4H-like3, Ma4CL-like1, Ma4CL-like10, MaCHS6-4-like, and MaFLS) and activated their expressions. Furthermore, the transient overexpression of MaABI5-like in ‘Fenjiao’ banana fruit and ectopic expression in tomato plants enhanced cold tolerance and upregulated fatty acid desaturation and flavonoid synthesis-related gene transcript levels. The reduced expression of MaABI5-like by virus-induced gene silencing in ‘Fenjiao’ banana increased chilling injury and downregulated the expression of fatty acid desaturation and flavonoid synthesis-related genes. Thus, the study indicates that MaABI5-like regulates ABA-induced cold tolerance by increasing unsaturated fatty acid and flavonoid content.
Modulating climacteric intensity in melon through QTL stacking
Authors: Santo Domingo Miguel , Areco Lorena , Mayobre Carlos , Valverde Laura , Martín-Hernández Ana Montserrat , Pujol Marta , Garcia-Mas Jordi 
Published: June 2022

Abstract
Fruit ripening is one of the main processes affecting fruit quality and shelf life. In melon there are both climacteric and non-climacteric genotypes, making it a suitable species to study fruit ripening. In the current study, in order to fine tune ripening, we have pyramided three climacteric QTLs in the non-climacteric genotype “Piel de Sapo”: ETHQB3.5, ETHQV6.3 and ETHQV8.1. The results showed that the three QTLs interact epistatically, affecting ethylene production and ripening-related traits such as aroma profile. Each individual QTL has a specific role in the ethylene production profile. ETHQB3.5 accelerates the ethylene peak, ETHQV6.3 advances the ethylene production and ETHQV8.1 enhances the effect of the other two QTLs. Regarding aroma, the three QTLs independently activated the production of esters changing the aroma profile of the fruits, with no significant effects in fruit firmness, soluble solid content and fruit size. Understanding the interaction and the effect of different ripening QTLs offers a powerful knowledge for candidate gene identification as well as for melon breeding programs, where fruit ripening is one of the main objectives.
Epigenetic reprogramming of H3K27me3 and DNA methylation during leaf-to-callus transition in peach
Authors: Zheng Beibei , Liu Jingjing , Gao Anqi , Chen Xiaomei , Gao Lingling , Liao Liao , Luo Binwen , Ogutu Collins Otieno , Han Yuepeng 
Published: June 2022

AbstractPlant tissues are capable of developing unorganized cell masses termed calluses in response to the appropriate combination of auxin and cytokinin. Revealing the potential epigenetic mechanisms involved in callus development can improve our understanding of the regeneration process of plant cells, which will be beneficial for overcoming regeneration recalcitrance in peach. In this study, we report on single-base resolution mapping of DNA methylation and reprogramming of the pattern of trimethylation of histone H3 at lysine 27 (H3K27me3) at the genome-wide level during the leaf-to-callus transition in peach. Overall, mCG and mCHH were predominant at the genome-wide level and mCG was predominant in genic regions. H3K27me3 deposition was mainly detected in the gene body and at the TSS site, and GAGA repetitive sequences were prone to recruit H3K27me3 modification. H3K27me3 methylation was negatively correlated with gene expression. In vitro culture of leaf explants was accompanied by DNA hypomethylation and H3K27me3 demethylation, which could activate auxin- and cytokinin-related regulators to induce callus development. The DNA methylation inhibitor 5-azacytidine could significantly increase callus development, while the H3K27me3 demethylase inhibitor GSK-J4 dramatically reduced callus development. These results demonstrate the roles of DNA methylation and H3K27me3 modification in mediating chromatin status during callus development. Our study provides new insights into the epigenetic mechanisms through which differentiated cells acquire proliferative competence to induce callus development in plants.
Mindfulness Intervention Decreases Frequency and Severity of Flares in Inactive Ulcerative Colitis Patients: Results of a Phase II, Randomized, Placebo-Controlled Trial
Authors: Jedel Sharon , Beck Todd , Swanson Garth , Hood Megan M , Voigt Robin M , Gorenz Annika , Jakate Shriram , Raeisi Shohreh , Hobfoll Stevan , Keshavarzian Ali 
Published: June 2022

Abstract

Background
Ulcerative Colitis (UC) is a chronic, inflammatory disease, characterized by symptomatic periods (flare) interspersed with asymptomatic periods (remission). Evidence suggests that psychological stress can trigger flare. Studies have shown that mindfulness interventions (MI) reduce stress, foster more adaptive coping, and improve quality of life, but have been minimally used for UC patients. The objective of this study was to determine whether participation in an MI results in improvements in UC disease course and inflammatory cascades, mindfulness, perceived stress, and other psychological outcomes in inactive UC patients with limited or no exposure to past MI.

Methods
Participants were randomized to an 8-week MI or control group. Biological and psychological assessments were performed at baseline, post 8-week course, and at 6- and 12-months.

Results
Forty-three participants enrolled. The MI increased the state of mindfulness and mindfulness skills, decreased perceived stress and stress response in patients with inactive UC. The MI intervention significantly decreased the incidence of flare over 12 months (P &lt; .05). None of the UC patients in the MI flared during 12 months, while 5 of 23 (22%) control group participants flared during the same period.

Conclusions
MIs could be considered as adjuvant treatment for a subset of UC patients with high perceived stress and low state of mindfulness.
The trial was registered at clinicaltrials.gov as NCT01491997.