bluuebunny/crossref_metadata_2025_split huggingface dataset:
Periodontitis may impair the homeostasis of systemic bone through regulation of gut microbiota in ApoE−/− mice
Authors: Li Lili , Wang Min , Bao Jun , Wang Nannan , Huang Yuezhen , He Shasha , Chen Bin , Yan Fuhua 
Published: July 2022

AbstractAimTo investigate whether periodontitis impacts bone homeostasis via gut microbiota regulation.Materials and MethodsExperimental periodontitis was induced by ligatures (LIG group). ApoE−/− mice were employed as a model with weakened bone homeostasis. Bone turnover was evaluated through micro‐computerized tomography, haematoxylin and eosin‐stained sections, osteoblast and osteoclast biomarkers in the bone and serum. Gut microbiota was analysed through 16S ribosomal RNA gene sequencing. Serum concentrations of cytokines were detected by enzyme‐linked immunosorbent assay. The role of gut microbiota was evaluated through their transplantation into antibiotic‐treated mice.ResultsPeriodontitis significantly increased the number of osteoclasts and the expression of the osteoclast biomarkers in the proximal tibia of ApoE−/− mice, with the RANKL/OPG (receptor activator of nuclear factor‐κB ligand/osteoprotegerin) ratio significantly increased, which indicated the osteoclastic activity overwhelmed osteogenesis. Meanwhile, periodontitis altered the composition of gut microbiota and induced low‐grade inflammation in the colon and blood circulation. Interestingly, the concentration of circulating tumour necrosis factor‐α, interleukin (IL)‐6, IL‐1β, IL‐17A, and monocyte chemotactic factor‐1 were positively correlated with faecal α1‐antitrypsin and calprotectin, as well as serum OPG and RANKL. Furthermore, transplantation of gut microbiota from mice with periodontitis to antibiotic‐treated mice could partially re‐capitulate the phenotypes in the bone and colon.ConclusionPeriodontitis may impair systemic bone homeostasis through gut microbiota.
Evolution and dosage compensation of nucleolar organizing regions (NORs) mediated by mobile elements in turtles with female (ZZ/ZW) but not with male (XX/XY) heterogamety
Authors: Montiel Eugenia E. , Badenhorst Daleen , Lee LingSze , Valenzuela Nicole 
Published: July 2022

AbstractUnderstanding the evolution and regulation of nucleolar organizing regions (NORs) is important to elucidate genome structure and function. This is because ribosomal gene (rDNA) copy number and activity mediate protein biosynthesis, stress response, ageing, disease, dosage compensation and genome stability. Here, we found contrasting dosage compensation of sex‐linked NORs in turtles with male and female heterogamety. Most taxa examined exhibit homomorphic rRNA gene clusters in a single autosome pair (determined by 28S rDNA fluorescence in situ hybridization), whereas NORs are sex‐linked in Apalone spinifera, Pelodiscus sinensis and Staurotypus triporcatus. Full‐dosage compensation upregulates the male X‐NOR (determined via silver staining—AgNOR) in Staurotypus (who lacks Y‐NOR) compared with female X‐AgNORs. In softshell Apalone and Pelodiscus, who share homologous ZZ/ZW micro‐chromosomes, their enlarged W‐NOR is partially active (due to 28S rDNA invasion by R2 retroelements), whereas their smaller Z‐NOR is silent in females but active in both male‐Zs (presumably because the W‐NOR meets cellular demands and excessive NOR activity is costly). We hypothesize that R2 disruption favoured W enlargement to add intact 28S‐units, perhaps facilitated by reduced recombination during sex chromosome evolution. The molecular basis of the potentially adaptive female Z‐silencing is likely intricate and perhaps epigenetic, as non‐ribosomal Z genes are active in Apalone females. Yet, Emydura maquarii exhibit identical heteromorphism in their autosomal NOR (R2 invaded 28S‐units and the small‐autosome NOR is silent), suggesting that the softshell turtle pattern can evolve independent of sex chromosome evolution. Our study illuminates the complex sex chromosome evolution and dosage compensation of non‐model systems that challenges classic paradigms.
Microstructure of rice (Oryza sativa L.) and kinetics in hydrothermal process
Authors: Nacimento Kauyse Matos , Balbinoti Thaisa Carvalho Volpe , Jorge Luiz Mario de Matos , Jorge Regina Maria Matos 
Published: July 2022

AbstractRice hydration is the main unit operation in both parboiled and germinated rice industries. Despite its importance, few studies investigate its basic principles, related to phenomena and physicochemical rice changes during hydration step in each one of these processes. Aiming to contribute to these processes, was a mass transfer kinetic study associated to identification of moisture incorporation mechanisms and alterations in the starch during the paddy rice hydration. The observed mass transfer in the process was directly influenced by the system temperature, showing changes in absorption capacity over time. A tracer pigment added to the hydration medium revealed that the mechanism of the water entrance into the grain was through diffusion through the grain coating and by capillarity through the fissures. The thermal analysis and scanning electron micrographs (SEM) showed that hydration at 55 and 65°C presented partial and total gelatinization of the starch, respectively. The models addressed allowed describing the moisture gain for various thermal conditions. The generalized model obtained was able to predict the experimental moisture at any process time for any of the investigated temperatures with a 5% maximum deviation. This research contributes significantly to the understanding of the phenomena involved in rice hydration, aiding in the development of operational parameters; they also point to a mathematical model that is able to represent the experimental data in a manner that is simple, quick, and inexpensive, particularly for industrial use.Practical ApplicationsThis study presents a paramount contribution to the time–temperature binomial establishment in the rice hydration process, since it provides as a process tool, the kinetic hydration model of moisture incorporation, as well as reveals the alterations in the grain microstructure. The discoveries can boost industries that process paddy rice, especially those located in poorer areas that lack knowledge and information, helping to improve operating conditions. Such a context justifies the investigation of models that may be easily applied, perform well, and are inexpensive, such as is the model presented in the present work, which may be applied by industries to monitor and also specify optimal operation conditions.
Identifying strawberry appearance quality by vision transformers and support vector machine
Authors: Zheng Hao , Wang Guohui , Li Xuchen 
Published: July 2022

AbstractThe strawberry is a highly nutritional and beneficial cash crop, and its appearance quality sorting is a crucial step in the production process. Manual identifying and sorting, however, are subjective and more time consuming. In the field of image classification, vision transformers (ViT) have shown better performance. In this article, an extensive evaluation of the ViT models for the identification of strawberry appearance quality is presented. Moreover, to balance the accuracy and computation time of the ViT model, we propose a ViT‐based method that uses fine‐tuned ViT‐B/32 to extract the class token and imports it into the support vector machine (SVM) to identify strawberry. Besides, the class token is imported into the SVM with different kernel functions to evaluate their performance. The experimental results show that the highest recognition accuracy of original ViT models is ViT‐L/16, which can reach 97.38%. The application of the linear kernel function is more suitable in this work. The accuracy of original ViT‐B/32 is 93.7% and the proposed method improves the accuracy by 4.4% up to 98.1%. Furthermore, the required computation time of the proposed method is only 62.13 s, which is faster than other models. Therefore, the proposed method demonstrates enhanced robustness and universality, especially for abnormal and ripe strawberries.Practical ApplicationsThe identification of strawberry appearance quality is a labor‐intensive task. Traditional methods mainly rely on manpower, which has high cost and low efficiency. Therefore, this work uses the fine‐tuned ViT‐B/32 to extract features and import them into SVM to identify the appearance quality of strawberries, which obtains the identification results more accurate and efficient. This study could facilitate the development of smart picking equipment for strawberries.
Proinflammatory role of basophils in oxazolone‐induced chronic intestinal inflammation
Authors: Inaba Kenichi , Higashiyama Masaaki , Watanabe Chikako , Tomioka Akira , Ito Suguru , Tanemoto Rina , Mizoguchi Akinori , Nishii Shin , Wada Akinori , Sugihara Nao , Hanawa Yoshinori , Horiuchi Kazuki , Akita Yoshihiro , Okada Yoshikiyo , Kurihara Chie , Narimatsu Kazuyuki , Komoto Shunsuke , Tomita Kengo , Karasuyama Hajime , Satoh Takahiro , Hokari Ryota 
Published: July 2022

AbstractBackground and AimThe functions of basophils have not been elucidated until recently because of their rarity. However, with recent developments in basophil‐specific antibodies and basophil‐deficient animals, the roles of basophils in various diseases related to chronic inflammation have been clarified. In this study, we aimed to investigate the roles of basophils in human ulcerative colitis (UC) and oxazolone (OXA) colitis using genetically engineered Mcpt8DTRmice.MethodsImmunohistochemical staining of human colon specimens was performed to examine the involvement of basophils in the pathogenesis of UC. We examined the correlation between the number of infiltrating basophils and the UC endoscopic index of severity (UCEIS), Mayo score, and Matts score. We also examined the correlation between eosinophil count and basophil infiltration. In murine experiments, we examined whether basophil infiltration was involved in OXA‐induced colitis and whether basophil depletion improved inflammation in Mcpt8DTRmice.ResultsColonic basophil infiltration was significantly increased in patients with UC. There were significant correlations between UCEIS, Mayo score, Matts score, and the number of infiltrating basophils. In murine OXA‐induced colitis, a significant increase in basophil infiltration was observed. When basophils were depleted by diphtheria toxin in Mcpt8DTRmice, inflammation improved significantly and mRNA expression of some proinflammatory cytokines, including Tnf‐α and Ifn‐γ decreased significantly.ConclusionBasophil infiltration correlated with endoscopic, clinical, and pathological scores in human UC independently of eosinophil infiltration, and depletion of basophils ameliorated mucosal inflammation in murine OXA‐induced colitis, collectively suggesting that basophils exert a proinflammatory role in chronic intestinal inflammation such as UC.
InMode Evoke radiofrequency hands‐free facial remodeling for skin rejuvenation
Authors: Hendricks Aleksi J. , Farhang Sheila Z. 
Published: July 2022

AbstractDemand for facial contouring procedures has increased dramatically in recent years. Common regions of concern for patients seeking a rejuvenated, more youthful appearance include the cheeks, jawline, submental area, and neck. Radiofrequency technology offers a non‐invasive and effective method of improving skin laxity and subcutaneous fat in these areas. The Evoke system from InMode Aesthetics is a radiofrequency modality designed to address the lower face and neck that stimulates collagen remodeling for skin tightening and thermal lipolysis for improved facial contour. While alternate technologies for radiofrequency facial remodeling are commercially available, Evoke is the first of its kind in introducing a reliable, operator‐independent hands‐free device developed based on InMode's proprietary Acquire, Control, and Extend technology targeting the deep fibro‐septal network to provide optimal and consistent outcomes.
A patient with a mucinous borderline ovarian tumor after fertility‐sparing surgery in whom puncture fluid cytology on oocyte retrieval led to a diagnosis of recurrence
Authors: Yoshiba Takahiro , Takei Yuji , Manaka Yumi , Suzuki Tatsuya , Fujiwara Hiroyuki 
Published: July 2022

AbstractThe patient was an unmarried nulliparous 21‐year‐old female who was referred to our hospital with an abdominal mass. Bilateral ovarian tumors with a solid component were detected and both were suspected to be ovarian cancer. Since the patient strongly wished to preserve fertility, we performed left salpingo‐oophorectomy, right cystectomy, and omentectomy. A postoperative histopathological examination revealed that the bilateral ovarian tumors were mucinous borderline tumors. The patient selected oocyte cryopreservation. Oocyte retrieval from the right ovary was performed 2 years after surgery, and six oocytes were obtained. The puncture of a small cyst revealed mucinous fluid, not normal follicular fluid; therefore, puncture fluid cytology was performed. A recurrent mucinous borderline ovarian tumor was suspected. Right salpingo‐oophorectomy was performed and a recurrent mucinous borderline ovarian tumor was diagnosed. Puncture fluid properties need to be considered when there is an opportunity for oocyte retrieval after fertility‐sparing surgery.
How Elementary In‐School Play Opportunities Relate to Academic Achievement and Social‐Emotional Well‐Being: Systematic Review
Authors: Burson Sheri L. , Castelli Darla M. 
Published: July 2022

ABSTRACTBACKGROUNDPlay is essential for child growth and development, and may have academic and social‐emotional implications. To understand the relationship between in‐school play opportunities and academic achievement (AA) and social‐emotional well‐being among elementary school students, a systematic review of the current in‐school play literature was conducted.METHODSKeywords were input across 7 search engines. After screening, 20 peer‐reviewed publications related to play, AA, and social‐emotional well‐being met the inclusion criteria for this review.FINDINGSAcross the included publications, recess was the only in‐school play opportunity assessed. Overall, student behavior and social‐emotional outcomes benefited from more recess while mixed results were found between recess participation and academic outcomes. However, more recess was not detrimental to AA.CONCLUSIONThe findings from this review support the need for recess as part of a whole child approach to education that may mitigate the negative outcomes of sitting through long periods of instruction. More research is needed on the AA and social‐emotional effects of other types of in‐school play opportunities (ie, centers, play stations, and other breaks). Findings from this review indicate that recess is a necessary part of the elementary school day.
Expression of insulin‐like growth factor genes in olive flounder, Paralichthys olivaceus, fed a diet with partial replacement of dietary fish meal
Authors: Moon Ji‐Sung , Oh Dae Han , Park Su‐Jin , Seo Jin‐Song , Kim Dong‐Uk , Moon Seong‐Hwan , Park Hung Sik , Lim Sang‐Gu , Lee Bong‐Joo , Hur Sang‐Woo , Lee Kyeong‐Jun , Nam Taek Jeong , Choi Youn Hee 
Published: July 2022

AbstractThis study analyzed the expression of growth‐related genes of olive flounder, Paralichthys olivaceus, fed a low‐fish meal (FM) diet to investigate the replacement of the FM diet in the field. The expression of growth hormone (GH)/insulin‐like growth factor (IGF) axis genes in the brain/pituitary/liver and plasma hormone concentrations were measured. A basal experimental diet was formulated using 63% FM and 12% soybean meal as the primary protein sources, and then two other diets were made by replacing 20% and 30% of the FM with soy protein concentrate, tankage meal, and poultry by‐products meal. Each diet was fed to duplicate groups of juvenile flounder (150 ± 3.0 g) twice a day. After 20 weeks, the plasma growth‐related hormone concentrations were similar between the control and FM20 groups. Moreover, GH/IGF axis gene mRNA expression in the brain/pituitary/liver was similar between the control and FM20 groups. Immunoblotting of muscle and liver showed the same pattern. This study showed the utility of replacing 20% of the FM diet and supports the possibility of field application.
Impact of disturbance and dietary shift on gastrointestinal bacterial community and its invertebrate host system
Authors: Kivistik Carmen , Tammert Helen , Kisand Veljo , Käiro Kairi , Herlemann Daniel P. R. 
Published: July 2022

AbstractThe gut microbiome is one of the most important sites of host–microbe interactions, however, mechanisms governing the responses of host‐associated microbes to changing environmental conditions are poorly understood. To address this, we investigated individual and combined effects of dietary changes and increase in salinity (from freshwater to salinity 3) or antibiotic concentration on the gastrointestinal bacterial community of the aquatic snail Ampullaceana balthica. In parallel, the energy reserves of the host were quantified. A change of natural food source to biofilm forming green algae Scenedesmus obliquus as well as the combined treatment of salinity and S. obliquus decreased the richness and changed the composition of the A. balthica gastrointestinal bacterial community. In these treatments Pseudomonas became the dominant bacterium. However, energy reserves of the host were higher in these treatments compared to the reference aquaria specimens and the combined treatment of antibiotics with S. obliquus. The presence of antibiotics inhibited the dominance of Pseudomonas and resulted in lower energy reserves despite S. obliquus feeding. Therefore the host seems to be able to adapt and replace its bacterial community composition to respond to mild changes in salinity and food source. Antibiotics in the water can disturb this self‐regulating mechanism. Our study underlines the ability of aquatic macroinvertebrates to respond to sudden changes in food source and mild shifts in salinity. Moreover, it emphasizes the strong impact of the food source on the gastrointestinal microbiome and the importance of generalists during disturbance.
What to expect when AGEP is induced by terbinafine? Case report and critical review of the literature
Authors: de Oliveira Gisele Viana , Maia Maria Luisa Peloso , Leão Felipe Augusto Azevedo , Sad Eduardo Fonseca , Miotto Isadora Zago , Silva Marcio Roberto , Ramos‐e‐Silva Marcia 
Published: July 2022

AbstractBackgroundAcute Generalised Exanthematous Pustulosis (AGEP) is a rash with multiple sterile intraepidermal or subcorneal non‐follicular pustules on edematous papules, with a sudden development and rapid evolution, triggered by drugs, vaccination, insect bites, exposure to mercury, and allergens.Objectives and MethodsWe describe a female patient who developed extensive and abnormally prolonged AGEP following exposure to terbinafine and SARS‐CoV vaccine. A detailed review of terbinafine‐induced‐AGEP cases was performed, with the aim of evaluating if the AGEP criteria would follow a different pattern when the disease is triggered by this drug. A PubMed search helped retrieve all terbinafine‐induced AGEP case reports. AGEP‐specific Sideroff criteria were analysed in terbinafine‐induced cases and compared to other trigger causes.ConclusionsWhen the AGEP causative drug was terbinafine, a delay in recovery was observed, compared to the existing AGEP criteria when other causes are considered. Terbinafine frequently leads to delayed resolution AGEP probably due to the presence of the drug in the skin for several weeks after exposure, even after discontinuation, and the disease severity may be potentialised by additional factors such as concomitant viral infections or vaccination.
Monetary Policy Uncertainty and Inflation Expectations*
Authors: Arce‐Alfaro Gabriel , Blagov Boris 
Published: July 2022

AbstractDo inflation expectations react to changes in the volatility of monetary policy? They have, but only until the global financial crisis. This paper investigates whether increasing the dispersion of monetary policy shocks, which is interpreted as elevated uncertainty surrounding monetary policy, affects the inflation expectation formation process. Based on US data since the 1980s and a stochastic volatility‐in‐mean structural VAR model, we find that monetary policy uncertainty reduces both inflation expectations and inflation. However, after the Great Recession this link has disappeared, even when controlling for the Zero Lower Bound.
Mid‐ and Long‐Term Comparison Analysis of Two Approaches for the Treatment of Level III or Higher Lenke–Silva Adult Degenerative Scoliosis: Radical or Limited Surgery?
Authors: Song Zhibo , Zhang Zhaoquan , Yang Xiaochen , Zhao Zhi , Li Tao , Bi Ni , Wang Yingsong 
Published: July 2022

ObjectiveAs the population in general is living longer, less invasive adult degenerative scoliosis (ADS) surgery that balances risks and benefits requires long‐term clinical outcomes to determine its strengths and weaknesses. We design a retrospective study to compare the postoperative mid‐ and long‐term outcomes in terms of efficacy, surgical complications, and reoperation rate of patients with ADS treated with two different surgical approaches (long‐segment complete reconstruction or short‐segment limited intervention).MethodsIn this retrospective study, 78 patients with ADS (Lenke–Silva levels III or higher), who accepted surgical treatment at our hospital between June 2012 and June 2019 were included. These patients were assigned to the long‐segment radical group (complete decompression with deformity correction involves ≥3 segments) and the short‐segment limited group (symptomatic segment decompression involves <3 segments). In addition, general information such as age, gender, fixed segment number, efficacy, radiographic parameters, and reoperation rate of patients in the two groups were compared and analyzed.ResultsThere were no significant differences between the two groups with regard to gender, follow‐up time, long‐term surgical complications and reoperation rate (P > 0.05). The mean age of patients in the long‐segment strategy group was 57.1 ± 7.9 years, with a mean number of fixed segments of 7.9 ± 2.4. The mean age of patients in the short‐segment strategy group was 60.8 ± 8.4 years, with a mean number of fixed segments of 1.4 ± 0.5. At the final follow‐up visit, the long‐segment radical group showed better results than the short‐segment limited group with regard to coronal Cobb angle, lumbar lordosis angle and sagittal balance (P < 0.05). The long‐segment strategy group had a higher implant‐related complication rate (P = 0.010); the adjacent segment‐related complication in the two groups showed no significant difference (P = 0.068).ConclusionConsidering the risk, rehabilitation pathway and costs of long‐segment radical surgery, short‐segment limited intervention is a better strategy for patients who cannot tolerate the long‐segment surgery, improving symptoms and maintaining efficacy in the mid‐ and long‐term, and not increasing the reoperation rate.