bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterisation of a TatA/TatB binding site on the TatC component of theEscherichia colitwin arginine translocase
Authors: Severi Emmanuele , Batista Mariana Bunoro , Lannoy Adelie , Stansfeld Phillip J. , Palmer Tracy 
Published: December 2022

ABSTRACTThe twin arginine transport (Tat) pathway exports folded proteins across the cytoplasmic membranes of prokaryotes and the thylakoid membranes of chloroplasts. InEscherichia coliand other Gram-negative bacteria, the Tat machinery comprises TatA, TatB and TatC components. A Tat receptor complex, formed from all three proteins, binds Tat substrates, which triggers receptor organisation and recruitment of further TatA molecules to form the active Tat translocon. The polytopic membrane protein TatC forms the core of the Tat receptor and harbours two binding sites for the sequence-related TatA and TatB proteins. A ‘polar’ cluster binding site, formed by TatC transmembrane helices (TMH) 5 and 6 is occupied by TatB in the resting receptor and exchanges for TatA during receptor activation. The second binding site, lying further along TMH6 is occupied by TatA in the resting state, but its functional relevance is unclear. Here we have probed the role of this second binding site through a programme of random and targeted mutagenesis. Characterisation of three stably produced TatC variants, P221R, M222R and L225P, each of which is inactive for protein transport, demonstrated that the substitutions did not affect assembly of the Tat receptor. Moreover, the substitutions that we analysed did not abolish TatA or TatB binding to either binding site. Using targeted mutagenesis we introduced bulky substitutions into the TatA binding site. Molecular dynamics simulations and crosslinking analysis indicated that TatA binding at this site was substantially reduced by these amino acid changes, however TatC retrained function. While it is not clear whether TatA binding at the TMH6 site is essential for Tat activity, the isolation of inactivating substitutions indicate that this region of the protein has a critical function.
The impact of narcissism, self-confidence and auditor’s characteristics on audit report readability
Authors: Salehi Mahdi , Dalwai Tamanna , Arianpoor Arash 
Published: December 2022

PurposeThe present study aims to assess the impact of narcissism, self-confidence and auditor's characteristics on audit report readability for companies listed on the Tehran Stock Exchange.Design/methodology/approachThe study’s statistical population comprises firms listed on the Tehran Stock Exchange. The present research used a systematic elimination method, and 1,162 firm-year observations were obtained for seven years from 2012 to 2018. Three variables including auditor tenure, audit fee and audit specialization are used for measuring auditing features. The Fog index is used as a proxy for measuring audit report readability. In addition, in this paper, four regressions, including fixed effects, random effects, pooled and T+1, are used to estimate reliable coefficients.FindingsThe findings show a negative and significant relationship between auditor’s characteristics (tenure, fee and specialization) and audit report readability. Moreover, the variables of the auditor’s narcissism, self-confidence and mandatory auditor change have a positive and significant association with audit report readability. This study lends support to the theories of personality disorder and behavioral decision.Originality/valueSince narcissism and self-confidence are two characteristics that shape an individual’s character and personality, some involved behavioral factors in auditors’ characteristics contribute to their decisions. The effects of these should be detected to enhance the decision-making process. The said factors significantly impact audit report readability. Hence, this paper attempts to assess the effect of the said factors on audit report readability.
Understanding and preserving Indigenous agricultural knowledge: a suggested way forward for future research
Authors: Radcliffe Chris , Parissi Cesidio 
Published: December 2022

Purpose
Indigenous farmers have, for centuries, applied practices which maintained resilient and sustainable landscapes. Thus, understanding and preserving the agricultural knowledge of Indigenous farmers may enhance the knowledge base of sustainable agriculture. The purpose of this paper is to review current research in the fields of Indigenous knowledge and sustainability to present a research approach which enables a cohesive global way forward for future research projects seeking to understand and preserve Indigenous agricultural knowledge.

Design/methodology/approach
This study applied thematic analysis to review 57 research studies in the field of Indigenous knowledge and sustainability. Key themes were identified from four overarching criteria: research methodology, data input, output and outcomes.

Findings
The findings revealed a range of commonalities among the 57 research studies reviewed. This study proposes the research should continue to seek to understand and preserve Indigenous knowledge, however, research needs to go beyond simply documenting Indigenous knowledge. The way forward requires research of Indigenous agricultural knowledge to establish databases, digital repositories (including oral, video, visual) and online repositories with globally shared access, whilst acknowledging and acting in partnership with Indigenous farmers and their communities.

Originality/value
To the best of the authors’ knowledge, this is an original study which has practical implications for enhancing research outcomes with regard to preservation of Indigenous knowledge. The findings of this study may be used to influence research policy formulation and implementation.
Aerodynamic optimization of the exhaust system of an aft-mounted boundary layer ingestion propulsor
Authors: Matesanz-García Jesús , Piovesan Tommaso , MacManus David G. 
Published: December 2022

Purpose
Novel aircraft propulsion configurations require a greater integration of the propulsive system with the airframe. As a consequence of the closer integration of the propulsive system, higher levels of flow distortion at the fan face are expected. This distortion will propagate through the fan and penalize the system performance. This will also modify the exhaust design requirements. This paper aims to propose a methodology for the aerodynamic optimization of the exhaust for novel embedded propulsive systems. To model the distortion transfer, a low order throughflow fan model is included.

Design/methodology/approach
As the case study a 2D axisymmetric aft-mounted annular boundary layer ingestion (BLI) propulsor is used. An automated computational fluid dynamics approach is applied with a parametric definition of the design space. A throughflow body force model for the fan is implemented and validated for 2D axisymmetric and 3D flows. A multi-objective optimization based on evolutionary algorithms is used for the exhaust design.

Findings
By the application of the optimization methodology, a maximum benefit of approximately 0.32% of the total aircraft required thrust was observed by the application of compact exhaust designs. Furthermore, for the embedded system, it is observed that the design of the compact exhaust and the nacelle afterbody have a considerable impact on the aerodynamic performance.

Originality/value
This paper presents a novel approach for the exhaust design of embedded propulsive systems in novel aircraft configurations. To the best of the authors’ knowledge, this is the first detailed optimization of the exhaust system on an annular aft-mounted BLI propulsor.
An assessment of key sustainability competencies: a review of scales and propositions for validation
Authors: Annelin Alice , Boström Gert-Olof 
Published: December 2022

Purpose
The purpose of this paper is to review and provide propositions about survey assessment tools of the key sustainability competencies (KSCs) of education for sustainability. UNESCO points out how education plays an important role in transforming societies towards a sustainable future and achieving the United Nations’ sustainable development goals. To plan education for sustainability, teachers need to know the students’ competencies for sustainability before they come to class. Thus, a formative assessment about student competence for sustainability is needed.

Design/methodology/approach
Firstly, a structured literature review of assessment tools used to measure sustainability competencies by questionnaire survey is presented. Secondly, the authors’ conceptualise how the competencies influence each other and provide propositions for future research.

Findings
The literature demonstrates that there is much ambiguity between prior research about the scales used and what they represent. A lack of validation across disciplines is apparent and an assessment tool that includes all eight KSCs could benefit education for sustainability. Future research could investigate how the competencies influence each other and which drivers are stronger for each discipline across different countries. A formative assessment tool can address this need.

Originality/value
The findings provide a new analysis about questionnaire assessment tools used in prior research to measure sustainability competence. The authors’ offer a discussion about the strengths and weaknesses found in prior research and propose suggestions for future research. Their conceptualisation also provides propositions for validating the KSCs presented in a recent framework.
Genealogy tourism and city tourism in Coimbra. Proposal for a Jewish culture route
Authors: Santos Norberto , Oliveira Moreira Claudete , Silveira Luís 
Published: December 2022

Purpose
Tourism in Coimbra today is influenced by the fact that the Univer(s)city was distinguished as a World Heritage Site in 2013. The number of visits has grown very significantly in recent years, but the diversification of the tourist offer is still weak and unable to take advantage of existing resources. This paper aims to present genealogy tourism as an alternative urban cultural tourism in Coimbra.

Design/methodology/approach
Methodology involved mapping the Jewish culture elements in the city of Coimbra, and a route was outlined and proposed.

Findings
Genealogy tourism resources are identified in the historic centre of the city. These alternative spaces need urban rehabilitation and (re)functionalisation, which allowed the authors to rethink tourism in Coimbra. They are the motivation to visit for all urban cultural tourists, especially Israelis/Jews, and provide contact with places where the experiences of ancestors combine with the history and memory of places, with recent discoveries and the elements of Jewish culture in the city.

Originality/value
It is concluded that the quantity, diversity, authenticity and singularity of the heritage resources that bear witness to the Jewish presence in Coimbra are sufficient assets to create a route, to enrich the tourist experience in the city and to include the destination in the Sephardic routes.
Initial evaluation of a mobile therapeutic game for adolescent anxiety disorders
Authors: Barnes Steven , Prescott Julie , Adams Joseph 
Published: December 2022

Purpose
This study aims to evaluate a novel mobile therapeutic videogame for adolescents with anxiety disorders (ADs), combining elements of cognitive-behavioural therapy and attention-bias modification, in terms of both its therapeutic efficacy over a controlled intervention and two-month follow-up, as well as the extent and implications of self-directed play.

Design/methodology/approach
A within-groups design with two parallel conditions [clinical anxiety (N = 16) and subclinical/at-risk (N = 15)] were measured on both self-reported anxiety and threat-detection bias (TDB) across three timepoints (pre- and post-intervention and two-month follow-up).

Findings
Significant reductions were observed in both self-reported state and trait anxiety and TDB over the course of the two-week intervention, which were maintained at follow-up. Engagement in self-directed play during the follow-up period significantly predicted outcomes at two-month follow-up for clinical participants.

Originality/value
To the best of the authors’ knowledge, this paper represents the first of its kind to evaluate a mobile therapeutic game designed with and solely for adolescents with ADs. This study also represents the first of its kind to examine the extent and implications of self-directed play for outcomes.
Father Cícero’s water: is it really for drinking?
Authors: Santana Jannsen , Pimentel Rodrigo Oliveira , Adelino Brandão Halana , Valdevino Antonio Messias 
Published: December 2022

Purpose
This teaching case aims to provide in-class discussions on the commercialization and consumption of goods in spiritual markets. This teaching case is a narrative based on facts with a fictitious plot reporting the journeys of the launch, commercialization and consumption of mineral water in bottles in the shape of Father Cícero – a religious leader in Northeast Brazil – produced by Blue Spring Mineral Water in Juazeiro do Norte’s pilgrimage spiritual market.

Design/methodology/approach
Primary and secondary data sources were gathered. Two focus groups with pilgrims and semi-structured interviews with Blue Spring leaders and a local Church representative were conducted as primary data. As secondary data, a dossier was created holding content from newspapers and blogs online on the focal product and data from the company’s official website and social networks.

Findings
Throughout the case, the strategies and challenges of the production and commercialization of this religiously appealing product and the consumer practices adopted by different consumer profiles are unfolded.

Originality/value
This case intends to be a useful pedagogical tool to discuss the creation, production, commercialization and consumption of goods in spiritual markets. The case allows students to experience Blue Spring’s managers’ point of view regarding the decisions of production and commercialization of the focal product. In a broad sense, this case intends to inform future marketers of the importance of balancing commercialization in religious contexts.
Online learning attributes on overall tourism and hospitality education learning satisfaction: Tourism Agenda 2030
Authors: Li Chang (Cherise) , Agyeiwaah Elizabeth 
Published: December 2022

Purpose
Promoting “Quality Education” as part of Sustainable Development Goal 4 requires educational stakeholders to understand the nature of online learning from the learners’ perspective. This study aims to explore the asymmetric effects of online learning attributes on overall learning satisfaction in tourism and hospitality education.

Design/methodology/approach
The authors adopt the three-factor satisfaction theory and the three analytical steps penalty–reward contrast analysis, impact range performance analysis (IRPA) and impact asymmetry analysis. The authors then juxtapose the results of such analytical approaches to the traditional importance–performance analysis (IPA) for further insights.

Findings
Traditional IPA suggests nine high importance and performance online attributes. However, the IRPA reveals that only four attributes fall within high performance and high range of impact on satisfaction. The attribute “secure” had a low performance despite a significant impact on satisfaction.

Research limitations/implications
By exploring asymmetric effects, scholars, service providers and participants of online education can have a deeper insight into the nature and dynamic effect of attributes on overall satisfaction.

Originality/value
By exploring the asymmetric impacts of online learning attributes on overall learning satisfaction, the findings provide a novel insight into the multidimensionality of online learning attributes.
Effect of autoclave–cooling cycles combined pullulanase on the physicochemical and structural properties of resistant starch from black Tartary buckwheat
Authors: Xu Qinglian , Zheng Faying , Yang Ping , Tu Ping , Xing Yage , Zhang Ping , Liu Hong , Liu Xiaocui , Bi Xiufang 
Published: December 2022

AbstractA starch‐rich portion is produced as a by‐product of black Tartary buckwheat processing. The effect of enzymatic combined with autoclaving–cooling cycles (one, two, or three times) on the physicochemical and structural properties of black Tartary buckwheat type 3 resistant starch (BRS) was evaluated. The autoclaving–cooling cycles enhanced solubility and reduced swelling, with the BRS content increasing from 14.12% to 25.18%. The high crystallinity of the BRS reflected a high molecular order. However, increasing the number of autoclaving–cooling cycles did not result in higher BRS content. The highest BRS yield in the autoclaved starch samples was 25.18% after double‐autoclaving–cooling cycles. Furthermore, the autoclaving–cooling cycles altered the crystalline structure of black Tartary buckwheat, and the subsequent crystallinity changed from 36.33% to 42.05% to 38.27%. Fourier‐transform infrared spectroscopy shows that the number of cycles results in more efficient double‐helical packing within the crystalline lamella. Principal component analysis showed that the autoclaving–cooling cycle treatment leads to significant changes in the molecular structure of resistant starch (RS). These results indicated that autoclaving–cooling cycles might be a feasible way for producing RS from black Tartary buckwheat starch with better structural stability to expand their application range.
Green synthesized TiO2 nanoparticles: Structural characterization and photoinduced antifungal activity against P. steckii
Authors: Xing Yage , Fan Xiangfeng , Li Xuanlin , Xu Qinglian , Tang Jing , Wu Lin , Wang Qi , Bi Xiufang , Liu Xiaocui 
Published: December 2022

AbstractThis study synthesized titanium dioxide (TiO2) nanoparticles (NPs) from mango leaf extract and investigated the features and antibacterial capabilities of three different. The microscopic morphological observation, scanning electron microscopy, and transmission electron microscopy results showed that all three NPs showed agglomeration phenomenon, and the TN‐1 sample existed as large agglomerates, whereas the agglomeration phenomenon of TN‐3 sample was improved by the modified, without large agglomerates. The biosynthetic TN‐2 and TN‐3 NPs were spherical and uniform in size, whereas those of the TN‐3 sample was the smallest, ranging from 10 to 30 nm. X‐ray diffraction and Raman spectroscopy results exhibited that these were highly pure anatase NPs. The result of ultraviolet (UV)–visible–near‐infrared spectral analysis showed that the TN‐2 and TN‐3 samples displayed higher UV absorption properties than the TN‐1 samples and were highest in the modified NPs, which was more suitable for preparing chitosan‐based nanocomposite material in future experiments and studies. The colony diameters of the TN‐1, TN‐2, and TN‐3 treatment groups were 7.99, 7.80, and 6.86 mm, respectively, after 120 min of UV light induction at a wavelength of 365 nm. Significant differences were evident between the TN‐3 and the other two groups (p < 0.05), indicating that the TN‐3 sample more effectively inhibited Penicillium steckii than the other TiO2 NPs.Practical ApplicationNanomaterials coated film preservation is widely used in fruit and vegetable preservation. In this paper, TiO2 nanomaterials will be green synthesized using mango leaf and structurally characterized, whereas antibacterial tests will be conducted against the mango fruit–specific bacterium Penicillium steckii, which will provide a theoretical basis for the storage and preservation of mango.
High plasma concentrations of acyl‐coenzyme A binding protein (ACBP) predispose to cardiovascular disease: Evidence for a phylogenetically conserved proaging function of ACBP
Authors: Montégut Léa , Joseph Adrien , Chen Hui , Abdellatif Mahmoud , Ruckenstuhl Christoph , Motiño Omar , Lambertucci Flavia , Anagnostopoulos Gerasimos , Lachkar Sylvie , Dichtinger Silvia , Maiuri Maria Chiara , Goldwasser François , Blanchet Benoit , Fumeron Frédéric , Martins Isabelle , Madeo Frank , Kroemer Guido 
Published: December 2022

AbstractAutophagy defects accelerate aging, while stimulation of autophagy decelerates aging. Acyl‐coenzyme A binding protein (ACBP), which is encoded by a diazepam‐binding inhibitor (DBI), acts as an extracellular feedback regulator of autophagy. As shown here, knockout of the gene coding for the yeast orthologue of ACBP/DBI (ACB1) improves chronological aging, and this effect is reversed by knockout of essential autophagy genes (ATG5, ATG7) but less so by knockout of an essential mitophagy gene (ATG32). In humans, ACBP/DBI levels independently correlate with body mass index (BMI) as well as with chronological age. In still‐healthy individuals, we find that high ACBP/DBI levels correlate with future cardiovascular events (such as heart surgery, myocardial infarction, and stroke), an association that is independent of BMI and chronological age, suggesting that ACBP/DBI is indeed a biomarker of “biological” aging. Concurringly, ACBP/DBI plasma concentrations correlate with established cardiovascular risk factors (fasting glucose levels, systolic blood pressure, total free cholesterol, triglycerides), but are inversely correlated with atheroprotective high‐density lipoprotein (HDL). In mice, neutralization of ACBP/DBI through a monoclonal antibody attenuates anthracycline‐induced cardiotoxicity, which is a model of accelerated heart aging. In conclusion, plasma elevation of ACBP/DBI constitutes a novel biomarker of chronological aging and facets of biological aging with a prognostic value in cardiovascular disease.
Comparison of a multidomain frailty index from routine health data with the hospital frailty risk score in older patients in an Australian hospital
Authors: Fujita Kenji , Lo Sarita Y. , Hubbard Ruth E. , Gnjidic Danijela , Hilmer Sarah N. 
Published: December 2022

AbstractBackgroundFrailty is an important determinant of health‐care needs and outcomes for people in hospital.ObjectivesTo compare characteristics and predictive ability of a multidomain frailty index derived from routine health data (electronic frailty index‐acute hospital; eFI‐AH) with the hospital frailty risk score (HFRS).MethodsThis retrospective study included 6771 patients aged ≥75 years admitted to an Australian metropolitan tertiary referral hospital between October 2019 and September 2020. The eFI‐AH and the HFRS were calculated for each patient and compared with respect to characteristics, agreement, association with age and ability to predict outcomes.ResultsMedian eFI‐AH was 0.17 (range 0–0.66) whilst median HFRS was 3.2 (range 0–42.9). Moderate agreement was shown between the tools (Pearson's r 0.61). After adjusting for age and gender, both models had associations with long hospital stay, in‐hospital mortality, unplanned all‐cause readmission and fall‐related readmission. Specifically, the eFI‐AH had the strongest association with in‐hospital mortality (adjusted odds ratio (aOR) 2.81, 95% confidence intervals (CI) 2.49–3.17), whilst the HFRS was most strongly associated with long hospital stay (aOR 1.20, 95% CI 1.18–1.21). Both tools predicted hospital stay >10 days with good discrimination and calibration.ConclusionsAlthough the eFI‐AH and the HFRS did not consistently identify the same inpatients as frail, both were associated with adverse outcomes and they had comparable predictive ability for prolonged hospitalisation. These two constructs of frailty may have different implications for clinical practice and health service provision and planning.