bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing Global Kidney Nutrition Care
Authors: Wang Angela Yee-Moon , Okpechi Ikechi G. , Ye Feng , Kovesdy Csaba P. , Brunori Giuliano , Burrowes Jerrilynn D. , Campbell Katrina , Damster Sandrine , Fouque Denis , Friedman Allon N. , Garibotto Giacomo , Guebre-Egziabher Fitsum , Harris David , Iseki Kunitoshi , Jha Vivekanand , Jindal Kailash , Kalantar-Zadeh Kamyar , Kistler Brandon , Kopple Joel D. , Kuhlmann Martin , Lunney Meaghan , Mafra Denise , Malik Charu , Moore Linda W. , Price S. Russ , Steiber Alison , Wanner Christoph , ter Wee Pieter , Levin Adeera , Johnson David W. , Bello Aminu K. 
Published: January 2022

Background and objectives
Nutrition intervention is an essential component of kidney disease management. This study aimed to understand current global availability and capacity of kidney nutrition care services, interdisciplinary communication, and availability of oral nutrition supplements.

Design, setting, participants, & measurements
The International Society of Renal Nutrition and Metabolism (ISRNM), working in partnership with the International Society of Nephrology (ISN) Global Kidney Health Atlas Committee, developed this Global Kidney Nutrition Care Atlas. An electronic survey was administered among key kidney care stakeholders through 182 ISN-affiliated countries between July and September 2018.

Results
Overall, 160 of 182 countries (88%) responded, of which 155 countries (97%) answered the survey items related to kidney nutrition care. Only 48% of the 155 countries have dietitians/renal dietitians to provide this specialized service. Dietary counseling, provided by a person trained in nutrition, was generally not available in 65% of low-/lower middle–income countries and “never” available in 23% of low-income countries. Forty-one percent of the countries did not provide formal assessment of nutrition status for kidney nutrition care. The availability of oral nutrition supplements varied globally and, mostly, were not freely available in low-/lower middle–income countries for both inpatient and outpatient settings. Dietitians and nephrologists only communicated “sometimes” on kidney nutrition care in ≥60% of countries globally.

Conclusions
This survey reveals significant gaps in global kidney nutrition care service capacity, availability, cost coverage, and deficiencies in interdisciplinary communication on kidney nutrition care delivery, especially in lower-income countries.
Diverse molecular phenotypes of ATP7B protein in Wilson disease: functional insights and therapeutic implications
Authors: Mukherjee Shashwata 
Published: January 2022

Wilson disease is a rare genetic disease causing copper accumulation due to mutations in the copper transporter protein ATP7B gene. Its varied clinical features result from toxic build-up of the ion primarily in liver and brain. The large number and variety of ATP7B mutations and influence of modifiers are suggested to account for only part of this heterogeneity. Exciting research on functional aspects is gradually elucidating the interesting behaviour of mutant ATP7B under different physiological conditions. Subtle perturbations in its delicate architecture have been shown to affect diverse functional properties of this copper pump. It is possible that compound heterozygotes bearing mutations with two different molecular phenotypes may partially complement each other’s defect, giving rise to unpredictable clinical presentation. These have tremendous potential in contributing to the clinical variability. More recent findings indicate the role of interacting partners in modulating the disease phenotype. The present review aims to outline the major strategies for in vitro evaluation of mutant ATP7B protein function, summarize the information on such studies in the literature, and gain new insights on the molecular behaviour and regulation of the protein. The functional consequence of mutations at 86 residue positions was reviewed through search in Pubmed and this information was analysed. A glimpse on studies at the transcription level is also provided. The development of new generation drugs to correct the underlying molecular defect is briefly discussed to emphasize how basic research to elucidate the molecular phenotype can have the potential to usher in the age of precision medicine for this disease.
Studies on the isolation and molecular characterization of Lactobacillus spp. from human breast milk and assessment of their probiotic potential
Authors: Chathyushya K. B. , Prakash Myakala Shiva , Y. Kodandapani Y. , Bheema B. , Babu J. J. G. , Rajkumar Rajkumar 
Published: January 2022

Human breast milk is a prominent source for microbiota development in the infant gastrointestinal tract. It is reported that probiotics in breast milk have a major role in the protection of the newborns and as well as infants when compared to formula milk/cow milk fed babies. The aim of the present study was to isolate and identify the probiotics from human breast milk. Fifty healthy women during the first week of postpartum were selected randomly and milk samples were collected aseptically. The samples were screened for probiotic microflora using microbiological methods, including serial dilution and plating on de Man-Rogosa-Sharpe agar. The isolates suspected to be probiotics were selected for morphological, biochemical and molecular characterization and bioinformatics analysis. They were further evaluated for their probiotic potential. Mixed microflora were found: commensal microorganisms like Streptococcus spp., Staphylococcus spp. and Lactobacillus spp. Twelve Lactobacillus spp. were isolated from breast milk, which were confirmed for genus and species by molecular methods and bioinformatics. These Lactobacilli were found to be acid resistant, tolerant to 0.3% bile and had ability to inhibit the growth of food-borne pathogens. A diversified bacterial population was present in human breast milk, and it was also found to be a rich source for beneficial probiotics, particularly Lactobacillus spp.
Tripleurospermum maritimum from a coastal shingle beach: nitrophilic status, tolerance to salinity and heavy metals
Authors: Ievinsh Gederts , Andersone-Ozola Una , Karlsons Andis , Osvalde Anita 
Published: January 2022

Tripleurospermum maritimum is a species characteristic for several protected sea coast habitats. The aim of the present study was to investigate possible nitrofilous status, tolerance to salinity and heavy metals, as well as the metal accumulation potential of T. maritimum plants from a sea-affected coastal habitat in controlled conditions. Plants were cultivated in an automated greenhouse in containers with a substrate made from garden soil and quartz sand. Three separate experiments were performed: (i) plants received combined treatment of three different doses of mineral fertilizer plus additional NO3‑N or NH4‑N; (ii) plants were treated with increasing concentration of NaCl; and (iii) plants were treated with increasing concentration of Cd or Pb. After seven weeks of cultivation, plant morphological characteristics, soluble ion concentration in tissue extracts and tissue Cd and Pb concentrations were measured. Increase in mineral nutrient availability stimulated shoot growth of T. maritimum plants. Addition of N fertilizer resulted in further increase of shoot biomass by 20 to 30%. Plants exhibited good tolerance to increased Na+ concentration, with only about 30% reduction in shoot biomass at 5 g L‑1. High electrolyte accumulation potential was evident in shoots, with preferred accumulation of K+ over Na+. Tolerance to heavy metals was very high, as growth of plants was not affected even in 100 mg L‑1 Cd treatment. Root biomass of plants treated with 500 mg L‑1 Pb was reduced only by 35%, while shoot growth was unaffected. Both Cd and Pb were preferentially accumulated in roots of T. maritimum, with concentration in aboveground parts being less than 10% from that in roots. In conclusion, the coastal accession of T. maritimum from a shingle beach is moderately nitrophylic with high salinity tolerance and very high tolerance to heavy metals, with characteristic exclusion of the metals from aboveground parts.
Bile Acids and Their Value for Central Nervous System
Authors: Shulpekova Yu. O. , Tkachenko P. E. , Shirokova E. N. , Damulin I. V. 
Published: January 2022

Aim. A review to highlight the bile acids importance as steroid mediators of nervous system activity and show the nervous system involvement in cholesterol metabolism and bile acids production.Key points. Presence of bile acid membrane and nuclear receptors and their activation role in mediating manifold metabolic processes have been established in various organs and tissues. Bile acid transporters are discovered in CNS. The animal brain under physiological conditions was found to contain about 20 bile acid types of likely innate origin suggested by their high contents; the bile acids spectrum in CNS differs significantly from blood plasma. Clinical and experimental works are conclusive about the CNS bile acids influence on mitochondrial membrane, their antioxidative role and, probably, steroid-mediator involvement in indirect regulation of memory, attention, motor functions and appetite.Conclusion. Bile acids act as pleiotropic signalling molecules affecting various tissues. The presence in CNS of various bile acid synthesis-related receptors and enzymes indicates their value in brain functioning and warrants research into their metabolism.
Mobile Game for Equality of Fractions for Elementary School Students
Authors: Wulandari Ayu , Amir Mohammad Faizal 
Published: January 2022

The low learning outcomes in the equality of fractions material are due to the unavailability of mobile technology game media that can train and explore the pattern of equality of fractions appropriately. This study aims to produce a valid, practical, and effective mobile game for equality of fractions (MoGEF) to improve learning outcomes on equality of fractions material. This study uses research and development methods through analysis, design, development, implementation, and evaluation. The subjects of this study were 29 students in the fourth grade of elementary school. Validity was measured by assessing two experts (mathematical education expert and information technology education expert). Practicality was measured through the results of the student response questionnaire, while effectiveness was measured through the posttest of student learning outcomes after using MoGEF. Data were collected using questionnaires and tests. The validity results obtained an average of 86.06% in the interval of 85.00% 100.00% with completely valid criteria. Practicality obtained an average of 4.659, which is in the interval of 2.50 3.25 with very practical criteria. Effectivenes shows the criteria of 79.83% berada pada interval 70.00% 85.00% dengan kriteria efektif. The effectiveness of the mobile game obtained a significance value of 2.127 > 2.048, which means it can improve the learning outcomes of elementary school students in the equality of fractions material. Therefore, the development of mobile games for equality of fraction is concluded to be very valid, very practical, and effective.
Dampak Pemberian Tugas Berbantuan Video Terhadap Perkembangan Motorik Halus Anak Usia Dini
Authors: Andriani Ni Luh Made Dian , Wiarta I Wayan 
Published: January 2022

Wabah Covid-19 menyebabkan pelaksanaan kegiatan belajar mengajar harus dilaksanakan secara daring. Namun, sekolah menerapkan system semi daring sehingga pada saat anak sekolah, pendidik dapat mengevaluasi dan mensinkronkan hasil karya pada saat full daring dan semi daring untuk mengetahui perkembangan motorik halus anak. Penelitian ini bertujuan untuk menganalisis penngaruh pemberian tugas berbatuan video di masa pandemic terhadap perkembangan motorik halus anak usia dini kelompok B. Rancangan eksperimen non-equivalent post-test only control group designPopulasi berdasarkan kelas B terdiri dari 3 kelas yakni B1, B2, B3 sehingga total populasi yaitu 77 orang anak. Sampel dipilih dengan metode random sampling. Data diperoleh berdasarkan metode non-tes dengan instrumen penelitian melalui lembar tes kuesioner. Metode analisis yang digunakan yaitu analisis statistik deskriptif, dengan melakukan uji hipotesis statistic dengan uji t. Berdasarkan hasil analisis diketahui bahwa terdapat perbedaan yang signifikan perkembangan motorik halus anak usia dini yang dibelajarkan menggunakan media audio visual dan kelompok anak yang dibelajarkan dengan pembelajaran konvensional di TK. Jadi, pemberian tugas di masa pandemi menggunakan media audio visual berpengaruh signifikan terhadap perkembangan motorik halus anak usia dini kelompok B.
Narrativas desviantes tramadas com palavras e linhas
Authors: Alfeld Elisabete 
Published: January 2022

O objeto de análise constitui-se no estudo de duas obras – vão e Moiras, autoria de Edith Derdyk –, livro de artista e instalação, manifestações artísticas interfaciadas com materiais de diversas procedências – a palavra, a linha branca de algodão, os bastões de ferro. O livro apresenta uma narrativa construída com um conjunto de palavras e frases – dispostas entre ‘vãos’, nos espaços em branco na linha da página. A instalação foi concebida especialmente para a área externa do SESC Ipiranga, cuja proposta foi enredar a linhas brancas de algodão pelos bastões fincados na parede do muro. O objetivo do estudo é apresentar um pensamento sobre poéticas de narrativas desviantes presentes no livro de artista e na instalação.
ACQUISITION OF SENTENCES BY A THREE-YEAR-OLD INDONESIAN BOY: A CASE STUDY ON M. GENTAMAS RANSI ALDEN
Authors: Hayani Fadhila . , Ginting Siti Aisyah , Husein Rahmad . 
Published: January 2022

This study was investigated about the acquisition of sentences by three-year-old Indonesian boy, M. Gentamas Ransi Alden. The objective of this study were to investigated the sentences that produced by three years old Indonesia boy, and to explain the reason for the sentences produced based on mood in a case study on M. Gentamas Ransi Alden. This research was conducted by using descriptive qualitative research design. The data were utterances from a three years old Indonesia boy and source of data taken from M. Gentamas Ransi Alden. For the duration of almost three months, the researcher observed and recorded the subject’s produced sounds, words, and sentences. The researcher also observed how the learner interacts with various linguistics inputs to see how the subject processes them. Finally, the researcher also observed him as interacts with people to determine the levels of the various constructs of his communicative competence. The result of the data showed that the subject already acquired the sentences based on mood, they are: 1) declarative sentences, 2) imperative sentences, 3) exclamatory sentences, and 4) interrogative sentences. In acquiring the second language, the research subject acquired the sentence during the process of imitating from their parents since they start over to speak. The subject used a declarative sentence to declare their statements, the interrogative sentence they use it to ask about information of something, the exclamatory sentences they use to shows about the emotion and also to declare their statement, and the imperative sentences to ask another to do something. Keywords: Children Sentence Acquisition, Kinds of Sentences, a Three Year Old, Indonesian Boy
STUDENTS ATTITUDE IN LEARNING ENGLISH CONVERSATIONS
Authors: Utami Sury . , Husein Rahmad . , Zainuddin Zainuddin . 
Published: January 2022

English conversation is an important lesson for Senior High School students in order to face the globalization effects. Many students considered English conversation as a controversial lesson through their positive and negative attitudes toward it. This study aimed to investigate the types of attitudes of the Senior High School students in learning English conversation, to describe the realization of the attitudes of Senior High School students in learning English conversations, and to explain the reason of the attitude which Senior High School students realized in the ways they are. The subjects of this study were 20 students consisting 12 females and 8 males at the age of 16-18 years old. The data were collected by observations and interviews then were analyzed using Interactive Models. The result showed that positive and negative attitude in learning English conversations, five types of realizations, and the reasons of the students realized their attitudes i.e. the language loyalty, language pride, and awareness of language norms. It was concluded that the students’ attitude varied as to positive and negative ones and realized in numerous manners affected by several factors. Keywords: Discourse Markers, Students Interactions, Nonformal Education, Conversation
The Effect of Dietary Addition of a Synbiotic After Long-Term Starvation on Certain Biochemical Parameters and Liver Structure in Common Carp (Cyprinus Carpio L.)
Authors: Zapryanova Dimitrinka , Urku Cigdem , Simeonov Radoslav , Atanasoff Alexander , Nikolov Galin , Sandeva Gergana 
Published: January 2022

Abstract
The prolonged starvation has many effects on the physiological and morphological processes in fish and interferes them with the ability to absorb nutrients and caloric intake. The aim of present study was to investigate the effect of long-term starvation and posterior dietary administration of commercial synbiotic consisting fructoligosaccharide (FOS) which used in combination with Enterococcus, Lactobacillus, Bifidobacterium and Lactococcus spp. for evaluation on some blood biochemical parameters and histological characteristics of common carp liver. Experimental fish after 5 months starvation period were subjected to the following feeding protocols: continuous starvation until 60 day (ST), re-feeding (FS) with dry composed diet (Aqua 1032 Swim) and refeeding with composed diet and synbiotic (FSS) for the following 60 days. Blood samples were used for analysis of the following plasma parameters: total protein, albumin, globulin, urea, creatinine, cholesterol, AST, ALT, glucose and serum for thyroid hormones. Histology analyses were performed on the carp liver samples. During the experimental period, fasting and refeeding were influenced significant on studied biochemical parameters, except glucose and urea. Also, the statistically largest (P <0.001) difference was observed in albumin and cholesterol values after refeeding. Our results reveal the changes in values of biochemical parameters during long-term starvation and refeeding with synbiotic and indicate signs of structural alterations of the liver in common carp.
Effect of Storage Time On the Hatchability of Eggs of Two-Line Dual-Purpose Combination for Production of Male Chickens for Meat
Authors: Petkov Evgeni , Popova Teodora 
Published: January 2022

Abstract
The aim of the study was to assess the effect of the storage time on the hatching traits of eggs obtained from a two-line dual-purpose combination for production of male chickens that will be utilized for meat. The trial was carried out in the experimental poultry farm of the Institute of Animal Science-Kostinbrod, with a total of 150 Lohmann Brown Classic layers, at the age of 54 weeks. Hatching eggs were gathered for three weeks and were stored for 5, 10 and 15 days at 15-18°C ambient temperature prior incubation. The storage time had no effect on the fertility of the set eggs, however, it adversely affected the hatchability. Prolonged storage led to significant decline in the total hatchability (P=0.0027) and the hatchability of the fertile eggs (P<0.0001) which was lowest after 15 days of storage prior incubation. The viability of the chickens was influenced by the storage of the eggs prior incubation (P<0.0001), and decreased considerably when the chicks were hatched from eggs stored for 15 days.
Evaluation of the Effect of Synbiotic in Piglets’ Diet on the Nutritional Quality of Pork
Authors: Saracila Mihaela , Untea Arabela Elena , Panaite Tatiana Dumitra 
Published: January 2022

Abstract
The purpose of the study was to increase the nutritional quality of pork, and to investigate the effect of the synbiotic on the oxidative stability of lipid. A 4 weeks experiment was performed on 8 weaned piglets (age 47 days, TOPIGS), divided into 2 groups (C, E). The control group (C) received a conventional diet, while the experimental group (E) included a diet supplemented with organic Zn and 10 g/kg synbiotic mixture. The productive parameters were evaluated. After slaughtered, muscle (tenderloin, shoulder, loin, ham and belly) and organs samples (liver, spleen and kidney) were collected. The muscle and organs samples were analysed regarding the proximate chemical analysis (dry matter, crude protein, crude fat, and ash). The antioxidant capacity and thiobarbituric acid reactive substances (TBARS) were performed on shoulder and ham samples at first day of the trial, 4 and 7 days after refrigeration. The crude fat determined in the loin samples, recorded a significant increase in the E group compared to the C group. The ash was higher in the shoulder samples from C group than in E group. At 7 days of refrigeration, the MDA concentration was significantly lower in the shoulder and ham samples from E group than those from C group. The conclusion was that the diet supplemented with organic Zn and 10 g/kg synbiotic mixture contributed to the delay of the lipid oxidation process of the shoulder and ham samples during the refrigeration period.
Herbal Plants as Feed Additives in Broiler Chicken Diets
Authors: Vlaicu Petru Alexandru , Panaite Tatiana Dumitra , Untea Arabela Elena , Idriceanu Lavinia , Cornescu Gabriela Maria 
Published: January 2022

Abstract
This study evaluated the effect of some herbal plants as natural feed additives on broiler production parameters, carcass parts, and breast meat quality. A total of 150 broiler chicks, Cobb 500 hybrid, were allocated to five treatments with 30 broilers /group, for 42 days. The experimental treatments were: Control (C); a diet containing 5% alfalfa meal (A); a diet containing 5% alfalfa meal and 1% basil (AB); a diet containing 5% alfalfa and 1% thyme (AT) and a diet containing 5% alfalfa and 1% sage (AS). Bodyweight was significantly (P<0.05) affected in AS group compared with C and A groups. Feed conversion ratio was significantly higher only in A and AB groups. Experimental groups had significantly greater (P<0.05) gizzard weight. The total polyphenols content and the antioxidant capacity were significantly (P<0.05) higher in AB, AT, and AS groups versus the C group. The cholesterol concentration was significantly (P<0.05) decreased in AB, AT, and AS meat samples compared with C and A samples. Instrumental colour measurements indicated significant alterations (P<0.05) among the groups regarding the values of L*, a*, b*, H°, C*, and ΔE*. The texture analysis showed that hardness, gumminess, and chewiness were significantly (P<0.05) higher in AB, AT, and AS groups than in C and A groups.