bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estratégias participativas para a intervenção em saúde: relato de experiência no pet-saúde graduasus
Authors: Belasco Isabel Cristina , Patiño Rafael Andres 
Published: February 2022

Este trabajo relata una experiencia de trabajo interdisciplinario desarrollada por profesores, estudiantes de una Licenciatura en salud, profesionales de la salud y actores sociales de una comunidad, dentro del Programa de Educación para el Trabajo PET-Salud GraduaSUS. La primera parte del proyecto se concentró en un diagnóstico participativo de la situación de salud en la ciudad de Porto Seguro, Brasil, a partir del cual fueron planeadas acciones de intervención, junto con la comunidad. Específicamente, el texto presenta una serie de talleres realizados con, estudiantes de una escuela de la región, que apuntaron la violencia como uno de los principales problemas de su territorio. Los talleres fueron ofertados como un dispositivo de reflexión, articulación y elaboración de experiencias de violencia y, al mismo tiempo, como un espacio de reflexión y cuestionamiento de prácticas de discriminación y de violación de los derechos humanos, con las que los jóvenes tienen contacto en su cotidianidad. El trabajo reafirmo la potencia de las estrategias intervención social participativas para producir transformaciones sociales y subjetivas, inclusive en relación a problemas el campo de salud.
Role of the Supporting Surface in the Thermodynamics and Cooperativity of Axial
Ligand Binding to Metalloporphyrins at Interfaces
Authors: Johnson Kristen N. , Chilukuri Bhaskar , Fisher Zachary E. , Hipps K.W. , Mazur Ursula 
Published: February 2022

Abstract:
Metalloporphyrins have been shown to bind axial ligands in a variety of environments,
including the vacuum/solid and solution/solid interfaces. Understanding the dynamics
of such interactions is a desideratum for the design and implementation of next generation
molecular devices which draw inspiration from biological systems to accomplish diverse tasks
such as molecular sensing, electron transport, and catalysis to name a few. In this article, we
review the current literature of axial ligand coordination to surface-supported porphyrin receptors.
We will focus on the coordination process as monitored by scanning tunneling microscopy
(STM) that can yield qualitative and quantitative information on the dynamics and binding
affinity at the single molecule level. In particular, we will address the role of the substrate and
intermolecular interactions in influencing cooperative effects (positive or negative) in the binding
affinity of adjacent molecules based on experimental evidence and theoretical calculations.
Profiling of Biologically Active Metabolites of Spergula fallax L. Using High-resolution UPLC-QTOF-MS
Authors: Ibrahim Taghreed , Hegazy Mostafa , El-Hela Atef 
Published: February 2022

Background:
Medicinal plants are recognized to have a wide range of important biological
activities, Spergula fallax L. is a well-known plant while phytochemical and biological activities
screening of the species are rare.

Objective::
This study aims to evaluate the antioxidant, anticáncer, antimicrobial, and antiviral activities
of the methanol extract (ME) of the aerial parts of Spergula fallax L. and its successive fractions;
n-hexane (HF), ethyl acetate (EAF), and n-butanol (BF) fractions and identify their biologically active
metabolites.

Method:
Ultra-performance liquid chromatography (UPLC) coupled to quadrupole high-resolution
time-of-flight mass spectrometry (QTOF-MS) was utilized to establish the metabolic profile of
Sergula fallax L. Total phenol and flavonoids content were determined colorimetrically using Folin
Ciocalteu and aluminum chloride reagents, respectively. 1,2-diphenyl-1-picrylhydrazyl (DPPH) was
utilized for estimation of antioxidant activity, cytotoxic activity was determined on Hep-G2, HCT-
116, and MCF-7 cell lines, and agar diffusion method was used for screening of antimicrobial activity
while cytopathic effect inhibition assay was utilized for antiviral assay.

Results:
Twenty-eight metabolites were identified, flavonoids were predominated (26), it was characterized
by the presence of four main aglycones (Apigenin, luteolin, quercetin and kaempferol) with
their O and/or C-glycosides based on its MS fragments. ME and EAF significantly scavenged DPPH
in a dose-dependent manner with IC50 16.99 and 18.74 μg.mL-1, respectively. Among the tested
samples, only ME and EAF showed significant cytotoxic activity against the tested cell lines with
IC50 ranging from 5.541- 9.509 μg.mL-1. All tested samples significantly inhibited the growth of the
tested bacterial strains with inhibition zone diameters ranging from 11.2- 26.4 mm and showed a
wide range of antiviral activity.

Conclusion:
Generally, S. fallax is potentially bioactive as evident by antioxidant, cytotoxic, antibacterial,
and antiviral assays. These activities are correlated with its identified metabolites. The
results proved that S. fallax has promising medicinal activities and would be examined in future clinical
trials for herbal therapy.
Combretastatin A4-loaded Poly (Lactic-co-glycolic Acid)/Soybean Lecithin Nanoparticles with Enhanced Drug Dissolution Rate and Antiproliferation Activity
Authors: Tao Jing , Cao Qing-Ri , Cao Yue , Ahmed Atef Mohammed Qasem , Du Huan-Huan , Sun Wei , Lu Xiaojuan , Xu Zhao 
Published: February 2022

Objective:
This study aimed to prepare combretastatin A4 (CA4)-loaded nanoparticles (CA4
NPs) using poly(lactic-co-glycolic acid) (PLGA) and soybean lecithin (Lipoid S100) as carriers, and
further evaluate the physicochemical properties and cytotoxicities of CA4 NPs against cancer cells.

Methods:
CA4 NPs were prepared using a solvent evaporation technique. The effects of formulations
on CA4 NPs were investigated in terms of particle size, zeta potential, encapsulation efficacy, and drug
loading. The physicochemical properties of CA4 NPs were characterized using transmission electron
microscopy, X-ray powder diffraction, differential scanning calorimetry, and Fourier transform infrared
spectra. The drug release from CA4NPs was performed using a dialysis method. In addition, the cytotoxicity
of CA4NPs against human alveolar basal epithelial (A549) cells was also evaluated.

Results:
CA4 NPs prepared with a low organic/water phase ratio (1:20) and high drug/PLGA mass ratio
(1:2.5) exhibited a uniform hydrodynamic particle size of 142 nm, the zeta potential of -1.66 mV, and
encapsulation efficacy and drug loading of 92.1% and 28.3%, respectively. CA4 NPs showed a significantly
higher release rate than pure CA4 in pH 7.4 phosphate-buffered solution with 0.5% Tween 80. It
was found that the drug molecules could change from the crystal state to an amorphous form when
loaded into the PLGA/Lipoid S100 matrix, and some molecular interactions could also occur between
the drug and PLGA. Importantly, CA4 NPs showed a remarkably higher antiproliferation activity
against A549 cancer cells compared to pure CA4.

Conclusion:
These results suggested the promising potential of PLGA/Lipoid S100 nanoparticles as the
drug delivery system of CA4 for effective cancer therapy.
Privileged Scaffolds Targeting Bromodomain-containing Protein 4
Authors: Wang Ru , Wang Yi-Ang , Xu Yun-Gen , Shi Lei 
Published: February 2022

Abstract:
In recent years, bromodomain-containing protein 4 (BRD4), a member of the bromodomain
and extra terminal domain (BET) family, has been one of the most widely studied targets.
BRD4 is a transcriptional regulation factor, which regulates cell transcription, marks mammalian
biological mitosis, regulates cell cycle, and plays an important role in the biological process of cancer
occurrence and development. It has been demonstrated that the imbalance or dysfunction of BRD4
expression leads to various types of cancers, including testicular gene nuclear protein melanoma,
acute myeloid leukemia, colon cancer, breast cancer, liver cancer, and midline cancer. Therefore,
inhibition of BRD4 has become a valuable approach in the treatment of these cancers. To date, there
are numerous BRD4 inhibitors in preclinical development, some of which have entered human clinical
trials. In this review, current progress in the development of privileged scaffolds designed as
BRD4 inhibitors will be discussed by focusing on structure-activity relationship, selectivity, and
mechanisms of action.
Tyrosinase Inhibition by Novel Benzimidazole-thione Schiff Base Derivatives
Authors: Zhou Wei , Tang Junyuan , Zhou Xinchong , Liu Jinbing 
Published: February 2022

Background:
Tyrosinase is the enzyme responsible for the conversion of tyrosine to dopaquinone,
which is related to melanoma, neurodegenerative disorders, freckles, pigmented acne and age spots.
Controlling the tyrosinase activity could be an important way for treating overproduction of melanin.

Objective:
The development of safe and specific tyrosinase inhibitors could be used to treat hypermelanosis.

Methods:
5-nitro-1H-benzo[d]imidazole-2(3H)-thione was synthesized from 4-nitro-o-phenylenediamine
and carbon disulfide. The nitro group of 5-nitro-1H- S-1 ESI-HRMS benzo[d]imidazole-2(3H)-thione was
reduced with iron powder. The 5-amino-1H-benzo[d]imidazole- 2(3H)-thione Schiff base derivatives
were obtained by the reaction of 5-amino-1H-benzo[d]imidazole-2(3H)-thione with substituted benzaldehyde.
The tyrosinase inhibitory activities were investigated. The studies of kinetic analysis, metalchelating
properties, docking and cytotoxicity were also performed.

Results:
All of the compounds showed strong tyrosinase inhibitory activities with 5-((4-nitrobenzylidene)
amino)-1H-benzo [d]imidazole-2(3H)-thione (S-4) as the best tyrosinase inhibitor with an IC50 value of
4.8 ± 1.4 nM. Compound S-4 exhibited mixed type inhibition of mushroom tyrosinase, with Ki 15 nM
and Kis 42 nM. Copper binding to S-4 was detected spectrophotometrically and 1-100 μΜ S-4 displayed
negligible cytotoxicity to murine B16 melanoma cells.

Conclusion:
Our results demonstrated that these benzimidazolethione Schiff base derivatives might be
promising candidates as tyrosinase inhibitors.
In Silico Studies to Develop New GSK3β Inhibitors Effective in the
Alzheimer's Disease
Authors: Ozkat Gozde Yalcin , Yildiz Ilkay 
Published: February 2022

Background:
Alzheimer's disease affects a large part of the world’s population by prolonging
the human life span and becoming an economic burden in the health system. Therefore, its treatment becomes
more and more important every day. With the insufficiency of existing drug molecules, new drug
targets are being searched. The most important of these is the Glycogen Synthase Kinase 3β enzyme,
which is thought to be of key importance in Tau hyperphosphorylation and Amyloid β accumulation
mechanisms.

Objective:
In this research, computational studies were conducted to develop a new GSK3β enzyme inhibitor.

Method:
Leading compounds suitable for pharmacophore models obtained by the 3D QSAR method
were scanned in databases. In silico ADME/Tox analyses were performed on the obtained molecules.

Results:
Although the three molecules (ENA99104, CNR13756, TIM405938) had strong Dock Scores
(42.869, 53.344, and 41.119, respectively) in molecular docking calculations, only the CNR13756 molecule
was found successful according to molecular dynamics simulations.

Conclusion:
All computational studies have revealed that the CNR13756 molecule can exhibit a therapeutic
scaffold property, thus obtaining a selective GSK3β inhibitor with minimal side effects.
Necrotizing Fasciitis After Total Hip Arthroplasty: A Rare Case Presentation
Authors: Aytekin Kürşad , Aytekin Feyza Yildiz 
Published: February 2022

Background:
Necrotizing fasciitis (NF) is a life-threatening disease. Arthroplasty pa-tients with underlying or preexisting conditions and chronic medication use are prone to infection. The current report aimed to present a 53 years old female patient who developed necrotizing fasciit-is one month after total hip arthroplasty.

Case presentation:
On the postoperative 15th day, she had wound discharge. She was given oral an-ti-biotherapy. At postoperative 1 month, her complaints increased. At emergency service, she expe-rienced extreme pain in her lower extremity with pseudoparalysis, and a hip X-ray revealed gas im-ages. She used prednisolone for 10 years and insulin for 15 years because of pemphigus vulgaris and diabetes mellitus. Clinical and laboratory findings led us to the diagnosis of necrotizing fasciit-is. Despite aggressive treatment, including debridement, prosthesis removal, and antibiotherapy, un-fortunately, the patient died 13 days after admission.

Conclusion:
Patients with an immune suppressive condition, long-term corticosteroid use, or multi-ple underlying diseases should be closely monitored after total hip arthroplasty surgery to avoid such a devastating complication.
Association between Statin Use and Poor Outcomes in COVID-19 Patients
with Diabetes Mellitus: A Systematic Review
Authors: Audrey Jessica , Wisnu Wismandari , Tahapary Dicky L. 
Published: February 2022

Background and Aims:
Diabetes mellitus, cardiovascular diseases, obesity, and dyslipidaemia
are considered risk factors for more severe forms of COVID-19 infection. Statins have
been widely used in such patients to prevent the occurrence of cardiovascular events and the associated
mortality. However, statin use has been suggested to promote a more severe form of infection.
This review aims to investigate the association between statin use and poor outcomes in
COVID-19 patients with diabetes.

Methods:
Literature search was performed in PubMed, CENTRAL, Scopus, and pre-print databases
(MedRxiv and BioRxiv), and studies published up to March 6th, 2021 have been reviewed.
Selected studies were then assessed for risk of bias with the Newcastle Ottawa Scale.

Result:
Four studies were included in the final analysis; all were retrospective studies. Two studies
reported a decreased risk of mortality with statin use, while one study reported opposite findings.
The other one did not find a significant association between statin use and poor COVID-19 outcomes.

Conclusion:
Available data suggest that statins may be safely administered to diabetic COVID-19
patients as the majority of evidence signifies statins to confer benefits and improve clinical outcomes
in COVID-19 patients.
Synthesis, Anticancer Activities, and Mechanism of N-heptyl-containing
Biguanide Derivatives
Authors: Wang Wei , Xiao Di , Zhou Sichun , Xu Simeng , Tang Xinyi , Zhou Xinchong , Liu Jinbing , Xu Cangcang , Peng Mei , Yang Xiaoping 
Published: February 2022

Background:
In recent years, the anticancer effects of biguanide drugs have received considerable
attention. However, the effective concentration of biguanide drugs to kill cancer cells is
relatively high. Thus, we focus on structural modification of biguanides to obtain better antitumor
candidates. A previous study in our laboratory has found that a biguanide compound containing the
n-heptyl group has potent anticancer activity. However, the effect of different substituents on the
benzene ringside of the biguanides on the anti-proliferative activity is unknown.

Objective:
A series of n-heptyl-containing biguanide derivatives whose benzene rings were modified
by halogen substitution based on the intermediate derivatization method were further synthesized to
find new compounds with improved antiproliferative activities.

Method:
Ten n-heptyl-containing biguanide derivatives were synthesized via established chemical
procedures. The activities of these derivatives were explored by MTT assay, clonogenic assay, and
scratch assay. The protein levels were detected via Western blotting to explore the underlying mechanisms.

Results:
The optimal biguanide derivatives 10a-10c, 11d exhibited IC50 values of 2.21-9.59 μΜ for
five human cancer cell lines, significantly better than the control drug proguanil. The results of clonogenic
and scratch wound healing assays also confirmed the inhibitory effects of derivatives 10a-
10c, 11d on the proliferation and migration of human cancer cell lines. Western blot results demonstrated
that one representative derivative, 10c upregulates the AMPK signal pathway and downregulates
mTOR/4EBP1/p70S6K.

Conclusion:
All biguanide derivatives containing n-heptyl groups are more active than proguanil,
indicating that the modification of n-heptyl-containing biguanide derivatives provides a novel approach
for the development of novel high efficient antitumor drugs.
Autistic Traits in Schizophrenia Spectrum Disorders
Authors: Šustová Petra , Rodriguez Mabel 
Published: February 2022

Background:
In recent years, there has been an emerging interest in the overlap
between schizophrenia and autism. The co-occurrence between these two disorders may be
seen not only at a diagnostic level but also as the presence of autistic traits in schizophrenia.
There has been a rapid rise in the number of studies focusing on autistic traits in schizophrenia
and their clinical associations.

Objective:
Here, we provide a review that summarizes the current findings and highlights
gaps that may be addressed by future research.

Results:
Higher levels of autistic traits have been associated with earlier age of onset, longer
duration of illness, resistance to treatment, and more severe cognitive deficit. However, regarding
social cognition and psychosocial functioning, the findings are mixed.

Conclusion:
Patients with prominent autistic traits seem to be more affected. The effect of
autistic traits on social cognition and psychosocial functioning remains unclear, as does their
relationship with negative symptoms. Therefore, longitudinal studies are highly needed.
PEMANFAATAN TEKNOLOGI AUGMENTED REALITY UNTUK PENGEMBANGAN BAHAN AJAR MATERI KOMPUTER JARINGAN
Authors: Nuraini Listia , Ratnawati Dwi 
Published: February 2022

ABSTRACT:This study aims to develop Augmented Reality based learning media on computer network subject. The research method used in developing educational games is the research and development (R&D) method adapted from Borg & Gall. The steps of study namely needs analysis, identification of product specifications, product development, product validation, product revision and product testing. The subject of this study was students of class X at Vocational High School. The object of this study was Augmented Reality based learning media on computer network. The test results indicated that this media is feasible to use. The score of product validation gained 73.07% from media experts while it gained 87.5% from material experts under very decent category. Moreover, it gained 85,26% from product users under very feasible category. In addition, the effectiveness test indicated that the result was quite effective with a percentage of 64.41%. Therefore, it could be implied that this learning media was feasible and effective to use in learning process.ABSTRAK:Tujuan penelitian ini adalah pengembangan media pembelajaran berbasis Augmented Reality pada mata pelajaran jaringan komputer. Metode penelitian yang digunakan dalam mengembangkan game edukasi yaitu metode research and development (R&D) yang diadaptasi dari Borg & Gall dengan beberapa tahapan. Adapun langkahnya adalah analisis kebutuhan, identifikasi spesifikasi produk, pengembangan produk, validasi produk, revisi produk dan uji coba produk. Subjek penelitian ini adalah siswa kelas X SMK. Objek penelitian adalah media pembelajaran jaringan komputer berbasis Augmented Reality. Hasil ujicoba menyatakan bahwa media ini layak digunakan dengan prosentasi dari ahli media sebesar 73,07%, ahli materi mendapat 87,5% dengan kategori sangat layak, sedangkan pengguna mendapatkan 85,26% dengan kategori sangat layak. Sedangkan uji efektivitas menyatakan cukup efektif dengan prosentase 64,41%, sehingga disimpulkan bahwa media pembelajaran ini layak dan efektif digunakan dalam pembelajaran.
Web-Based Cognitive Testing in Psychiatric Research: Validation and Usability Study
Authors: Lynham Amy Joanne , Jones Ian R , Walters James T R 
Published: February 2022

Background
Cognitive impairments are features of many psychiatric disorders and affect functioning. A barrier to cognitive research on psychiatric disorders is the lack of large cross-disorder data sets. However, the collection of cognitive data can be logistically challenging and expensive. Web-based collection may be an alternative; however, little is known about who does and does not complete web-based cognitive assessments for psychiatric research.

Objective
The aims of this study are to develop a web-based cognitive battery for use in psychiatric research, validate the battery against the Measurement and Treatment Research to Improve Cognition in Schizophrenia (MATRICS) Consensus Cognitive Battery, and compare the characteristics of the participants who chose to take part with those of the individuals who did not participate.

Methods
Tasks were developed by The Many Brains Project and selected to measure the domains specified by the MATRICS initiative. We undertook a cross-validation study of 65 participants with schizophrenia, bipolar disorder, depression, or no history of psychiatric disorders to compare the web-based tasks with the MATRICS Consensus Cognitive Battery. Following validation, we invited participants from 2 large ongoing genetic studies, which recruited participants with psychiatric disorders to complete the battery and evaluated the demographic and clinical characteristics of those who took part.

Results
Correlations between web-based and MATRICS tasks ranged between 0.26 and 0.73. Of the 961 participants, 887 (92.3%) completed at least one web-based task, and 644 (67%) completed all tasks, indicating adequate completion rates. Predictors of web-based participation included being female (odds ratio [OR] 1.3, 95% CI 1.07-1.58), ethnicity other than White European (OR 0.66, 95% CI 0.46-0.96), higher levels of education (OR 1.19, 95% CI 1.11-1.29), diagnosis of an eating disorder (OR 2.17, 95% CI 1.17-4) or depression and anxiety (OR 5.12, 95% CI 3.38-7.83), and absence of a diagnosis of schizophrenia (OR 0.59, 95% CI 0.35-0.94). Lower performance on the battery was associated with poorer functioning (B=−1.76, SE 0.26; P<.001).

Conclusions
Our findings offer valuable insights into the advantages and disadvantages of testing cognitive function remotely for mental health research.