bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Anticancer Effect of Zuojinwan on Gastric Cancer Through Multiple Signaling Pathway via miR-107/RUNX1T1 Axis
Authors: he bin , Rao Qi , Li Ruochan , peng xinyi , Xu Yundan , Zhao Gang 
Published: September 2022

Abstract
Background: Zuojinwan (ZJW), a famous Chinese medicine formula, has been widely used to treat gastric cancer (GC) which is the third leading cause of cancer-related death around the world.miR-107, a kind of small non-coding RNAs, play a vital role in the occurrence and development of GC.
Aim:A network pharmacology-based strategy combined with in vitro and in vivo experiment were employed to investigate the anticancer effect, potential targets, and molecular mechanism of ZJW against GC by miR-107.
Methods:Potential targets and signaling pathways of ZJW to treating gastric cancerby miR-107 were determined usingbioinformatics analysis from public databases.
miR-107 and its downstream protein expression levels in GC tissues and cell lines were detected by real-time reverse transcription polymerase chain reaction (RT-PCR)
or western blot,which were further confirmed by Luciferase reporter assay and
Pearson correlation test.The anticancer effect of ZJW in vitro and in vivo were examined by Nude mouse xenograft tumor model and MGC-803 cells. The possible antitumor mechanism of ZJW was studied by using western blot analysis.
Results:A total of 33 bioactive ingredients and 147 gene targets of ZJW acting on GC by miR-107 were identified. The miR-107 level was reduced and RUNX1T1 was down-expressed in GC samples.The expression of target was positively correlated with miR-107 in patients with GC.RUNX1T1 was targeted by miR-107.Since overexpression of miR-107 significantly decreased RUNX1T1 expression in MGC-803 cells, and knockdown of miR-107 enhanced RUNX1T1 expression. ZJW inhibited MGC-803 cells proliferation in dose- and time-dependent manner by activating PI3K/AKT/mTOR pathway and arresting the cell cycle in S phase through regulating the expression of Cyclin A, Cyclin B1 and Cyclin E. ZJW suppressed MGC-803 cells migration in dose-dependent manner by increasing E-cadherin expression. ZJW induced MGC-803 cells apoptosis by Fas death receptor and Bcl-2/Bax pathway. Tumor growth was attenuated by ZJW in nude mouse.miR-107 expression was downregulated in ZJW-treated group compared with untreated group, while the protein expression of RUNX1T1 was upregulated.
Conclusions: showed significant anti-tumor activity against gastric cancer MGC-803 cells through multiple signaling pathways via miR-107/RUNX1T1 axis both in vitro and in vivo.
Retinex-Based Light Source Generation for Relighting up Night Photography
Authors: Oishi Sou , Fukushima Norishige 
Published: September 2022

Abstract
The lighting up of buildings is one of the entertainments that make a city more colorful, and photographer sometimes changes the lighting by using photo-editing applications. This paper proposes a method for automatically performing such an application that follows the Retinex theory. The Retinex theory indicates that the complex scenes caught by the human visual system are decomposed into illumination and reflectance components. Many Retinex-based applications take much cost into the decomposition, while we show that the decomposition accuracy is less dependent on the final output for the nightscape relighting. First, we propose an extracting method of a relighting saliency map using Retinex with edge-preserving filtering. Second, we propose a sampling method to specify the lighting area in the limited area. We show that relighting within the limited area can be represented without illumination and reflectance decomposition. We then show that this fact can be exploited to realize this application using a simple Retinex decomposition of the single-scale Retinex with fast edge-preserving filters to represent various lighting effects. Experimental results show that the proposed sampling method is successful in keeping the illuminated points in bright locations and equally spaced.Also, the proposed various diffusion method can enhance night skyline photographs with various expressions. Our code and dataset are available at https://github.com/norishigefukushima/RelightingUpNightPhotography.
How to coordinate China's regional low-carbon digital economy with economic and social development: a composite system synergy perspective
Authors: Xing Zeyu , Huang Jing , Wang Jing 
Published: September 2022

Abstract
This paper creatively puts forward the theory of low-carbon digital economy from the perspective of resource flow, digital flow and energy flow, and studies the synergy effect of low-carbon digital development by constructing the synergy model of low-carbon digital development economic and social development. Based on theoretical research, this paper constructs a synergy model and evaluation index system between low-carbon digital development and economic and social development, and an empirical analysis was conducted based on data related to regional low-carbon digital development and economic and social development in China from 2014 to 2019. The empirical results show that the development of China's low-carbon digital economy is the main driving force for future economic and social development. Further, if the low carbon economy and the digital economy included in the stimulus policies can play a synergistic role,It will be able to play the greatest role in promoting economic and social development. And such a scenario is used as a practice in different regions of China in future. The research conclusion of this paper expands the theoretical system of low-carbon economy and digital economy, provides theoretical support for the path design of regional low-carbon digital economy development in China, and enriches the discussion on the construction of ecological civilization and the construction of a community with a common future for mankind.
Staphylococcus aureus in cow milk and milk products in Ambo and Bako towns, Oromia, Ethiopia: Prevalence, associated risk factors, hygienic quality, and antibiogram
Authors: Borena Bizunesh Mideksa , Gurmessa Feyera Tesema , Gebremedhin Endrias Zewdu , Sarba Edilu Jorga , Marami Lencho Megersa 
Published: September 2022

Abstract
Background
Staphylococcus aureus is one of the foodborne gram-positive bacterial pathogens that cause staphylococcal food poisoning worldwide and can contaminate foods of animal origin. The current study was conducted in Ambo and Bako towns, Ethiopia to estimate the prevalence, associated risk factors, hygienic quality, and antibiogram of Staphylococcus aureus (S. aureus) in cow’s raw milk and milk products.
Results
The overall prevalence of S. aureus from milk and milk products was 15.6% (94/601) with the highest prevalence detected in bulk tank raw milk at 17.50% (14/80) and the lowest in ‘Ergo’ at 13.11% (8/61). Age of cows, previous history of treatment, stage of lactation, udder washing, use of towel for individual cow, tick infestation, hygiene of the milker and utensils, and use of disinfectant were found to be statistically significant (P < 0.05) risk factors for S. aureus isolation at the individual cow level. Accordingly, a higher probability of getting infected with S. aureus was detected in cows aged 5 years old, crossbreeds, had a history of previous treatment, mid-lactation stage, no udder washing practiced, no individual towel used, tick infestation, and poor milking hygiene. Personnel hygiene, container hygiene, and use of disinfectants were the major risk factors that had a statistically significant association (p < 0.05) with bulk tank milk contamination with S. aureus. The maximum S. aureus counts at the farm and restaurant levels were 1.92x104 CFU/mL and 1.86x104 CFU/mL, respectively. The antimicrobial susceptibility test showed a higher resistance of S. aureus against amoxicillin (98.48%), oxacillin (98.48%), ampicillin (98.48%), cefoxitin (92.42%), and tetracycline (83.33%), of which 43.94% of isolates showed multidrug resistance. The high prevalence of oxacillin and cefoxitin-resistant isolates, which is indicative of the existence of Methicillin-Resistant Staphylococcus aureus (MRSA), was also detected in the current study.
Conclusion
Higher prevalence of S. aureus and the multidrug-resistant isolates in milk and milk products is detected in study areas. Therefore, to make milk and milk products safe for human consumption, hygienic handling of milk and milk products, regular surveillance of antimicrobial resistance, and prudent use of drugs are suggested.
High transcriptional activity and clinical correlations in eosinophils of patients with late-onset asthma
Authors: Lin Ting-Yu , Lo Chun-Yu , Chang Po-Jui , Lo Yu-Lun , Lee Chung-Shu , Chang Chih-Hao , Yu Chih-Teng , Yao Jonathan Huai , Lin Shu-Min 
Published: September 2022

Abstract
Background: The immunological features of eosinophils in early-onset asthma (EOA) differs from that in late-onset asthma (LOA). Clinical trials of anti-interleukin-5 (IL-5) treatment for severe eosinophilic asthma showed a better response for LOA patients than EOA patients. We wonder if the transcriptional activity of activated eosinophils was different in EOA and LOA.
Methods: Eosinophils obtained from well controlled EOA and LOA patients and normal subjects were compared in terms of the mRNA expression of activation-related genes (integrins α4 and β2, CCR3, CCR5, and IL-5Rα, and IL-17RA) and specific markers (IL-4Rα, prostaglandin D2 receptor 2, high-affinity IgE receptor, TGF-β1, aryl hydrocarbon receptor, and endosomal TLR-3, 7, and 9) in eosinophils activated by IL-5 or IL-17. The correlation between mRNA expression and clinical features and lung function were further analyzed.
Results: The transcriptional expression of most genes was higher in activated eosinophils from LOA patients than in those from EOA patients and normal subjects. After IL-17 stimulation, the expression of certain genes was higher in atopic EOA patients than in non-atopic EOA patients. Similar observation was noted in obese EOA patients. After IL-5 stimulation, the transcriptional expression of most genes in eosinophils from LOA patients was negatively correlated with indicators of lung function. These correlations were less pronounced in EOA patients: After IL-17 stimulation, some genes in EOA patients were negatively correlated with post-bronchodilator changes in lung function. There was no significant correlation between mRNA expression and treatment control levels among LOA or EOA patients
Conclusion: This study describes differences in the transcriptional active patterns of eosinophils and their correlation to atopy and obese by age of onset. High transcriptional activity in activated eosinophils and a negative correlation to lung function indicate the importance of eosinophils in the pathogenesis of LOA.
Characterization of various Appel polynomials sequences
Authors: Drissi Driss 
Published: September 2022

Abstract
In this paper, we characterize various sequences of the Appell polynomials by means of the formula of representation of powers viewed from a linear operator perspective. First we construct some Gauss-Weierstrass integral representation operators for various sequence of Appell polynomials such as Euler-Frobenius polynomials, Degenerate Bernoulli polynomials, Bell-Touchard polynomials and for the generalized Tempesta-Bernoulli polynomials. Secondly, we introduce the Appell polynomials as well as the Hurwitz zeta function as eigenfunctions (eigenvectors) of certain well chosen discrete version of Gauss-Weierstrass integral operators. Our approach is inspired by the Gauss-Weierstrass transform for Hermite polynomials.
Empirical Study on Financial Literacy, Investors’ Personality, Overconfidence Bias and Investment Decisions and Risk Tolerance as Mediator Factor
Authors: Hussain Shahid , Rasheed Abdul 
Published: September 2022

Abstract
The key objective of this research paper is to estimate the impact of financial literacy, investor’s personality and overconfidence bias on investment decisions by using risk tolerance as a mediator variable. Inclusive finance makes numerous financial products and services accessible and affordable to the micro-finance community, remarkably those absent from the conventional financial system. Financial literacy is a leading factor affecting an individual's ability to access financial services. This study employed Structural Equation Modeling to investigate whether financial literacy and other personality traits affect investment decisions. The Regression results showed that financial literacy, investors' personality, and overconfidence bias are significantly relevant to risk tolerance and investment decisions (β = 0.128***, S.D = 0.047, t = 2.746; p < 1%; β 0.378***, S.D = 0.051, t = 7.414, p < 1%; β 0.269***, S.D = 0.052, t = 5.155, p < 1%; β 0.195***, S.D = 0.054, t = 3.619, p < 1%; β 0.371***, S.D = 0.055, t = 6.706, p < 1%; β0.195***, S.D = 0.061, t = 3.190, p < 1%). As mediation results showed, risk tolerance plays a significant role in financial literacy, investors' personality, overconfidence bias, and investment decisions (β = 0.024**, S.D = 0.011, t = 2.15, p < 5%; β = 0.024**, S.D = 0.011, t = 2.17, p < 5%; β = 0.047**, S.D = 0.018, t = 2.55, p < 5%). The implications of this study also provide valued recommendations for regulatory institutions to improve financial inclusion in the emerging market context.

JEL Classification: G40, G41
Crispr/cas-mediated Genome Editing in Mice for the Development of Drug Delivery Mechanism
Authors: SOWBHAGYA RAMACHANDREGOWDA , MUKTHA HARSHA , RAMAKRISHNAIAH THIPPENAHALLI NARASIMHAIAH , SURENDRA ADAGUR SUDARSHAN , TANVI YESUDAS , NIVITHA KARAYI , RAJASHEKARA SOMASHEKARA 
Published: September 2022

Abstract
Background: To manipulate particular locations in the bacterial genome, researchers have recently resorted to a group of unique sequences in bacterial genomes that are responsible for safeguarding bacteria against bacteriophages. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) are two such systems, each of which consists of an RNA component and an enzyme component.
Methods and Results: This review focuses primarily on how CRISPR/Cas9 technology can be used to make models to study human diseases in mice. Creating RNA molecules that direct endonucleases to a specific position in the genome are crucial for achieving a specific genetic modification. CRISPR/Cas9 technology has allowed scientists to edit the genome with greater precision than ever before. Researchers can use knock-in and knock-out methods to model human diseases like Neurological, cardiovascular disease, and cancer.
Conclusions: In terms of developing innovative methods to discover ailments for diseases/disorders, improved CRISPR/Cas9 technology will provide easier access to valuable novel animal models.
Distribution fractions and potential ecological risk assessment of heavy metals in mangrove sediments of the Greater Bay Area
Authors: Zhou Huan-Zhan , Wang Jun-Feng , Jiang Hui-Min , Cai Ze-Xiang , Tang Guan-Hui , Song Ding , Liu Sheng-Teng , Xu ZhiMin 
Published: September 2022

Abstract
The restoration of mangrove forests in coastal wetlands of China were constantly established since 1990s. However, various pollutants, especially for heavy metals (HMs), discharged with wastewater might present a significant risk to mangrove forests during the restoration. In this study, sediments of five typical mangrove wetlands with varying restoration years and management measures were collected to evaluate the distribution fractions and potential ecological risk of HMs in the Greater Bay Area (GBA). Cd (0.2–1.6 mg/kg) was found in high concentrations in the exchangeable fraction (37.8–71.5%), whereas Cu (54.2–94.8 mg/kg), Zn (157.6-332.6 mg/kg), Cr (57.7-113.6 mg/kg), Pb (36.5–89.9 mg/kg) and Ni (29.7–69.5 mg/kg) primarily presented in residual fraction (30.8–91.9%). According to the geo-accumulation index (Igeo) analysis, sediment Cd presented high level of pollution (3 ≤ Igeo≤4), while Zn and Cu showed with moderately pollution (1 ≤ Igeo≤2). Besides, high ecological risk of Cd was found in sediments of five mangroves, with risk assessment code (RAC) ranging from 45.9 to 84.2. Redundancy analysis revealed that the content of NO3−-N was closely related to that of HMs in sediments and, pH value and NO3−-N concentration affected the distribution of HMs geochemical fractions. Furthermore, pollutants discharged from industrial activities rather than mangrove forest planting years determined the pollution levels of HMs. Fortunately, strict drainage standards for industrial activities in Shenzhen significantly availed for decreasing HMs contents in mangrove sediments. Therefore, future development of mangrove conversion and restoration should be linked to the water purification in the GBA.
Podocyturia: an Earlier Biomarker of Cardiovascular Outcomes
Authors: Habib Robert H. , Chehab Omar , Jalbout Nour Al , Tamim Hani , Makki Maha , Bejjani Martine El , Lima Joao , Badr Kamal F. , Eid Assaad 
Published: September 2022

Abstract
Urinary podocin and nephrin mRNAs were measured by quantitative PCR (podocyturia) in Type II diabetics with normal albumin excretion rates (AER) at baseline, at 3-4 years, and at 7 years. Development of cardiovascular disease (CVD) was collected as outcome. Visit 1 podocyturia was significantly higher in subjects who subsequently developed CVD versus those who did not. Visit 1 AER terciles exhibited similar time to CVD, in contrast with stepwise and substantial increases in CVD events predicted by Visit 1 podocyturia terciles. Covariate-adjusted hazard ratios were highest for podocin, intermediate for nephrin mRNAs, and lowest for AER. Podocyturia was also measured in patients with and without significant coronary obstruction, and in 480 normoalbuminuric subjects at the enrolment visit to the Multi-Ethnic Study of Atherosclerosis (MESA). Podocyturia > 3 x106 copies was associated with presence of obstructive coronary artery disease. In the MESA population, Visit 1 podocyturia was significantly higher in men, subjects with elevated BMI, and those with Type II DM.
Conclusions: Podocyturia is an earlier predictor of cardiovascular events than moderate albuminuria, is significantly higher in patients with obstructive coronary artery disease, and in subjects with established risk factors for CVD.
Global site-specific health impacts of fossil energy, steel mills, oil refineries and cement plants
Authors: Oberschelp Christopher , Pfister Stephan , Hellweg Stefanie 
Published: September 2022

Abstract
Climate change and particulate matter air pollution present major threats to human well-being by causing impacts on human health. Both are connected to key air pollutants such as carbon dioxide (CO2), primary fine particulate matter (PM2.5), sulfur dioxide (SO2), nitrogen oxides (NOx) and ammonia (NH3), which are primarily emitted from energy-intensive industrial sectors. We present the first study to consistently link a broad range of emission measurements for these substances with site-specific technical data, emission models, and atmospheric fate and effect models to quantify health impacts caused by nearly all global fossil power plants, steel mills, oil refineries and cement plants. The resulting health impact patterns differ substantially from far less detailed earlier studies due to the high resolution of included data, highlighting in particular the key role of emission abatement at individual coal-consuming industrial sites in densely populated areas of Asia (Northern and North-Eastern India, Java in Indonesia, Eastern China), Western Europe (Germany, Belgium, Netherlands) as well as in the US. Of greatest health concern are the high SO2 emissions in India, which stand out due to missing flue gas treatment and cause a particularly high share of local health impacts despite a limited number of emission sites. At the same time, the massive infrastructure and export capacity build-up in China in recent years is taking a substantial toll on regional and global health and requires more stringent regulation than in the rest of the world due to unfavorable environmental conditions and high population densities. The current phase-out of highly emitting industries in Europe is found not to have started with sites having the greatest health impacts. Our detailed site-specific emission and impact inventory is able to highlight more effective alternatives and to track future progress.
Investigation and application of multi-dimensional safety risk evaluation index system for coal mines in South China
Authors: You Qi , Yao Qingguo , Song Ruixin , Yu Kai , Xu Cuicui , Cao Haiying 
Published: September 2022

Abstract
The occurrence condition of coal seam differs greatly among various regions in China. The uneven development of coal mine safety production level in the North and South China is prominent. Due to the influence of personnel, equipment, mining technology, and other factors, the mortality rate per million tons of coal mines in A province in South China is 27 times the national average level. At present, not only the common factors such as enterprise safety production and government supervision, but also the pressure of regulatory and accountability, the pressure of social stability maintenance, and the external factors such as drastic changes in coal prices, coal supply guarantee, carbon peak and carbon neutralization, have imposed a double impact on coal mining enterprises and government supervision. The situation of coal mine safety production across China is very complex. In order to scientifically figure out the safety risk situation of coal mining enterprises in special periods and special areas, this study takes government supervisors, enterprise managers, underground front-line workers, and external factors as subjects and constructs a multi-dimensional safety risk evaluation system for coal mines in terms of work quality, work pressure, and economic factors. By horizontally and longitudinally comparing the scores of different main index factors, this system can timely discover the problems and weaknesses of supervisors at different levels and managers and front-line workers of different coal mining enterprises in the same region in work quality, work pressure, and economy, which can provide specific targeted suggestions for enterprises and governments on the safety management and safety supervision. This study not only pays attention to the hidden danger screening, risk control, and other factors that can directly reduce enterprise safety risks, but also considers the impact of personnel pressure, economic factors, external environment, and policy changes on the performance of safety production responsibilities by enterprise managers, government supervisors, and enterprise front-line workers. This is an innovation of this study. Finally, based on various subjective and objective weight calculation methods, a new comprehensive weight formula is proposed.