bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imperceptible, designable, and scalable braided electronic cord
Authors: Chen Min , Ouyang Jingyu , Jian Aijia , Liu Jia , Li Pan , Hao Yixue , Gong Yuchen , Hu Jiayu , Zhou Jing , Wang Rui , Wang Jiaxi , Hu Long , Wang Yuwei , Ouyang Ju , Zhang Jing , Hou Chong , Wei Lei , Zhou Huamin , Zhang Dingyu , Tao Guangming 
Published: November 2022

AbstractFlexible sensors, friendly interfaces, and intelligent recognition are important in the research of novel human-computer interaction and the development of smart devices. However, major challenges are still encountered in designing user-centered smart devices with natural, convenient, and efficient interfaces. Inspired by the characteristics of textile-based flexible electronic sensors, in this article, we report a braided electronic cord with a low-cost, and automated fabrication to realize imperceptible, designable, and scalable user interfaces. The braided electronic cord is in a miniaturized form, which is suitable for being integrated with various occasions in life. To achieve high-precision interaction, a multi-feature fusion algorithm is designed to recognize gestures of different positions, different contact areas, and different movements performed on a single braided electronic cord. The recognized action results are fed back to varieties of interactive terminals, which show the diversity of cord forms and applications. Our braided electronic cord with the features of user friendliness, excellent durability and rich interaction mode will greatly promote the development of human-machine integration in the future.
Structural and functional asymmetry of RING trimerization controls priming and extension events in TRIM5α autoubiquitylation
Authors: Herkules Frank , Yu Corey H. , Taylor Alexander B. , Dougherty Vi , Weintraub Susan T. , Ivanov Dmitri N. 
Published: November 2022

AbstractTRIM5α is an E3 ubiquitin ligase of the TRIM family that binds to the capsids of primate immunodeficiency viruses and blocks viral replication after cell entry. Here we investigate how synthesis of K63-linked polyubiquitin is upregulated by transient proximity of three RING domains in honeycomb-like assemblies formed by TRIM5α on the surface of the retroviral capsid. Proximity of three RINGs creates an asymmetric arrangement, in which two RINGs form a catalytic dimer that activates E2-ubiquitin conjugates and the disordered N-terminus of the third RING acts as the substrate for N-terminal autoubiquitylation. RING dimerization is required for activation of the E2s that contribute to the antiviral function of TRIM5α, UBE2W and heterodimeric UBE2N/V2, whereas the proximity of the third RING enhances the rate of each of the two distinct steps in the autoubiquitylation process: the initial N-terminal monoubiquitylation (priming) of TRIM5α by UBE2W and the subsequent extension of the K63-linked polyubiquitin chain by UBE2N/V2. The mechanism we describe explains how recognition of infection-associated epitope patterns by TRIM proteins initiates polyubiquitin-mediated downstream events in innate immunity.
Physics-informed deep learning approach for modeling crustal deformation
Authors: Okazaki Tomohisa , Ito Takeo , Hirahara Kazuro , Ueda Naonori 
Published: November 2022

AbstractThe movement and deformation of the Earth’s crust and upper mantle provide critical insights into the evolution of earthquake processes and future earthquake potentials. Crustal deformation can be modeled by dislocation models that represent earthquake faults in the crust as defects in a continuum medium. In this study, we propose a physics-informed deep learning approach to model crustal deformation due to earthquakes. Neural networks can represent continuous displacement fields in arbitrary geometrical structures and mechanical properties of rocks by incorporating governing equations and boundary conditions into a loss function. The polar coordinate system is introduced to accurately model the displacement discontinuity on a fault as a boundary condition. We illustrate the validity and usefulness of this approach through example problems with strike-slip faults. This approach has a potential advantage over conventional approaches in that it could be straightforwardly extended to high dimensional, anelastic, nonlinear, and inverse problems.
In situ monitoring of Lentilactobacillus parabuchneri biofilm formation via real-time infrared spectroscopy
Authors: Bajrami Diellza , Fischer Stephan , Barth Holger , Sarquis María A. , Ladero Victor M. , Fernández María , Sportelli Maria. C. , Cioffi Nicola , Kranz Christine , Mizaikoff Boris 
Published: November 2022

AbstractFoodborne pathogenic microorganisms form biofilms at abiotic surfaces, which is a particular challenge in food processing industries. The complexity of biofilm formation requires a fundamental understanding on the involved molecular mechanisms, which may then lead to efficient prevention strategies. In the present study, biogenic amine producing bacteria, i.e., Lentilactobacillus parabuchneri DSM 5987 strain isolated from cheese were studied in respect with biofilm formation, which is of substantial relevance given their contribution to the presence of histamine in dairy products. While scanning electron microscopy was used to investigate biofilm adhesion at stainless steel surfaces, in situ infrared attenuated total reflection spectroscopy (IR-ATR) using a custom flow-through assembly was used for real-time and non-destructive observations of biofilm formation during a period of several days. The spectral window of 1700–600 cm−1 provides access to vibrational signatures characteristic for identifying and tracking L. parabuchneri biofilm formation and maturation. Especially, the amide I and II bands, lactic acid produced as the biofilm matures, and a pronounced increase of bands characteristic for extracellular polymeric substances (EPS) provide molecular insight into biofilm formation, maturation, and changes in biofilm architecture. Finally, multivariate data evaluation strategies were applied facilitating the unambiguous classification of the observed biofilm changes via IR spectroscopic data.
Long-time simulations for fixed input states on quantum hardware
Authors: Gibbs Joe , Gili Kaitlin , Holmes Zoë , Commeau Benjamin , Arrasmith Andrew , Cincio Lukasz , Coles Patrick J. , Sornborger Andrew 
Published: November 2022

AbstractPublicly accessible quantum computers open the exciting possibility of experimental dynamical quantum simulations. While rapidly improving, current devices have short coherence times, restricting the viable circuit depth. Despite these limitations, we demonstrate long-time, high fidelity simulations on current hardware. Specifically, we simulate an XY-model spin chain on Rigetti and IBM quantum computers, maintaining a fidelity over 0.9 for 150 times longer than is possible using the iterated Trotter method. Our simulations use an algorithm we call fixed state Variational Fast Forwarding (fsVFF). Recent work has shown an approximate diagonalization of a short time evolution unitary allows a fixed-depth simulation. fsVFF substantially reduces the required resources by only diagonalizing the energy subspace spanned by the initial state, rather than over the total Hilbert space. We further demonstrate the viability of fsVFF through large numerical simulations, and provide an analysis of the noise resilience and scaling of simulation errors.
Hnf1b renal expression directed by a distal enhancer responsive to Pax8
Authors: Goea L. , Buisson I. , Bello V. , Eschstruth A. , Paces-Fessy M. , Le Bouffant R. , Chesneau A. , Cereghini S. , Riou J. F. , Umbhauer M. 
Published: November 2022

AbstractXenopusprovides a simple and efficient model system to study nephrogenesis and explore the mechanisms causing renal developmental defects in human.Hnf1b(hepatocyte nuclear factor 1 homeobox b), a gene whose mutations are the most commonly identified genetic cause of developmental kidney disease, is required for the acquisition of a proximo-intermediate nephron segment inXenopusas well as in mouse. Genetic networks involved inHnf1bexpression during kidney development remain poorly understood. We decided to explore the transcriptional regulation ofHnf1bin the developingXenopuspronephros and mammalian renal cells. Using phylogenetic footprinting, we identified an evolutionary conserved sequence (CNS1) located several kilobases (kb) upstream theHnf1btranscription start and harboring epigenomic marks characteristics of a distal enhancer in embryonic and adult renal cells in mammals. By means of functional expression assays inXenopusand mammalian renal cell lines we showed that CNS1 displays enhancer activity in renal tissue. Using CRISPR/cas9 editing inXenopus tropicalis, we demonstrated the in vivo functional relevance of CNS1 in drivinghnf1bexpression in the pronephros. We further showed the importance of Pax8-CNS1 interaction for CNS1 enhancer activity allowing us to conclude thatHnf1bis a direct target of Pax8. Our work identified for the first time aHnf1brenal specific enhancer and may open important perspectives into the diagnosis for congenital kidney anomalies in human, as well as modelingHNF1B-related diseases.
Maximum power extraction and DC-Bus voltage regulation in grid-connected PV/BES system using modified incremental inductance with a novel inverter control
Authors: AL-Wesabi Ibrahim , Zhijian Fang , Hussein Farh Hassan M. , A. Al-Shamma’a Abdullrahman , Dong Hanlin , M. Al-Shaalan Abdullah , Kandil Tarek 
Published: November 2022

AbstractLow ripples and variations in the DC-Bus voltage in single-phase Photovoltaic/Battery Energy Storage (PV/BES) grid-connected systems may cause significant harmonics distortion, instability, and reduction in power factor. The use of short-life electrolytic capacitor on the DC-Bus is considered a standard way for reducing these ripples and variations because of its large capacitance but results in short lifetime of the inverter. Replacing large electrolytic capacitors with small film capacitors can extend the lifetime of a PV/BES grid-connected system because small film capacitors have longer lifetime than large electrolytic capacitors. These film capacitors have low capacitance, which causes severe oscillations in the output current, and voltage drop due to huge ripples on the DC-Bus voltage. In this research, the main goal is to eliminate the output current ripples and voltage fluctuations associated with employing film capacitors. First, a modified incremental conductance (MIC) technique is proposed for tracking the maximum power by controlling the duty ratio of the DC-DC boost converter. Second, for the first time, a simple and novel d-q current regulation technique, which employs flowchart decision logic, is used in the DC-Bus control system for both the PV power system and the state of charge (SOC) of the BES. In this case, the DC-Bus controller is characterized by a cost-effective implementation because of its low sampling frequency. Although the presented approaches are successful in eliminating voltage distortion and fluctuations, they have unacceptable dynamic performance. Therefore, to improve the dynamic performance, BES was used to maintain a reliable and stable harvest from PV modules for varying loads while also increasing the dynamic performance of the overall system. The proposed PV/BES grid-connected systems, which employs a small 10-µF bus capacitor, is simulated and connected to the grid (230 V, 50 Hz). The DC-Bus voltage overshoot, undershoot and the total harmonics distortion (THD) of the output current for the proposed MIC are (1 V), (2.5 V) and (less than 5%), respectively. The average time response under rising radiation to track the global peak for MIC, traditional incremental conductance and variable step size incremental conductance are 1.403 s, 1.501 s and 1.113 s respectively. The obtained findings demonstrated the efficacy and superiority of the proposed d-q current control and MIC technique.
The contributions of ammonia oxidizing bacteria and archaea to nitrification-dependent N2O emission in alkaline and neutral purple soils
Authors: Hu Lei , Dong Zhixin , Wang Zheng , Xiao Liwei , Zhu Bo 
Published: November 2022

AbstractNitrification is believed to be one of the primary processes of N2O emission in the agroecological system, which is controlled by soil microbes and mainly regulated by soil pH, oxygen content and NH4+ availability. Previous studies have proved that the relative contributions of ammonia oxidizing bacteria (AOB) and archaea (AOA) to N2O production were varied with soil pH, however, there is still no consensus on the regulating mechanism of nitrification-derived N2O production by soil pH. In this study, 1-octyne (a selective inhibitor of AOB) and acetylene (an inhibitor of AOB and AOA) were used in a microcosm incubation experiment to differentiate the relative contribution of AOA and AOB to N2O emissions in a neutral (pH = 6.75) and an alkaline (pH = 8.35) soils. We found that the amendment of ammonium (NH4+) observably stimulated the production of both AOA and AOB-related N2O and increased the ammonia monooxygenase (AMO) gene abundances of AOA and AOB in the two test soils. Among which, AOB dominated the process of ammonia oxidation in the alkaline soil, contributing 70.8% of N2O production derived from nitrification. By contrast, the contribution of AOA and AOB accounted for about one-third of nitrification-related N2O in acidic soil, respectively. The results indicated that pH was a key factor to change abundance and activity of AOA and AOB, which led to the differentiation of derivation of N2O production in purple soils. We speculate that both NH4+ content and soil pH mediated specialization of ammonia-oxidizing microorganisms together; and both specialization results and N2O yield led to the different N2O emission characteristics in purple soils. These results may help inform the development of N2O reduction strategies in the future.
Plasma-generated reactive water mist for disinfection of N95 respirators laden with MS2 and T4 bacteriophage viruses
Authors: He Jinjie , Waring Michael , Fridman Alexander , Rabinovich Alexander , Bailey Charles , Fridman Gregory , Sales Christopher M. 
Published: November 2022

AbstractDue to the shortage of personal protective equipment (PPE) during the COVID-19 pandemic, the interest and demand for sterilization devices to reuse PPE has increased. For reuse of face masks, they must be effectively decontaminated of potential infectious agents without compromising its filtration ability during sterilization. In this study, we utilized an atmospheric pressure pulsed dielectric barrier discharge (DBD), combined with nebulized liquid microdroplets to generate plasma-activated mist (PAM). MS2 and T4 bacteriophages were used to conduct the decontamination tests on two types of N95 respirators. Results showed at least a 2-log reduction of MS2 and T4 on N95 respirators treated in one cycle with 7.8% hydrogen peroxide PAM and at least a 3-log reduction treated in 10% hydrogen peroxide PAM. In addition, it was found that there was no significant degradation in filtration efficiency of N95 respirators (3M 1860 and 1804) treated in 10% hydrogen peroxide PAM found after 20 cycles. In terms of re-useability of masks after treatment as determined, it was shown that the elastic straps of 3M 1804 were fragmented after 20 treatment cycles rendering them unusable, while the straps of 3M 1860 were not negatively affected even after 20 disinfection cycles.
Water oxidation couples to electrocatalytic hydrogenation of carbonyl compounds and unsaturated carbon–carbon bonds by nickel
Authors: Behrouzi Leila , Zand Zahra , Fotuhi Mobina , Kaboudin Babak , Najafpour Mohammad Mahdi 
Published: November 2022

AbstractArtificial photosynthesis, an umbrella term, is a chemical process that biomimetics natural photosynthesis. In natural photosynthesis, electrons from the water-oxidation reaction are used for carbon dioxide reduction. Herein, we report the reducion of aldehydes and ketones to corresponding alcohols in a simple undivided cell. This reaction utilized inexpensive nickel foam electrodes (1 cm2) and LiClO4 (0.05 M) as a commercially accessible electrolyte in an aqueous medium. Under electrochemical conditions, a series of alcohols (21 examples) produces high selectivity in good yields (up to 100%). Usage the current method, 10 mmol (1060 mg) of benzaldehyde is also successfully reduced to benzyl alcohol (757 mg, 70% isolated yield) without any by‑products. This route to alcohols matched several green chemistry principles: (a) atom economy owing to the use of H2O as the solvent and the source of hydrogen, (b) elimination of the homogeneous metal catalyst, (c) use of smooth reaction conditions, (d) waste inhibition due to low volumetric of by-products, and (e) application of safe EtOH co-solvent. Moreover, the ability of the system to operate with alkyne and alkene compounds enhanced the practical efficiency of this process.
Artificial intelligence workflow quantifying muscle features on Hematoxylin–Eosin stained sections reveals dystrophic phenotype amelioration upon treatment
Authors: Reinbigler Marie , Cosette Jérémie , Guesmia Zoheir , Jimenez Simon , Fetita Catalin , Brunet Elisabeth , Stockholm Daniel 
Published: November 2022

AbstractCell segmentation is a key step for a wide variety of biological investigations, especially in the context of muscle science. Currently, automated methods still struggle to perform skeletal muscle fiber quantification on Hematoxylin-Eosin (HE) stained histopathological whole slide images due to low contrast. On the other hand, the Deep Learning algorithm Cellpose offers new perspectives considering its increasing adoption for segmentation of a wide range of cells. Combining two open-source tools, Cellpose and QuPath, we developed MyoSOTHES, an automated Myofibers Segmentation wOrkflow Tuned for HE Staining. MyoSOTHES enables solving segmentation inconsistencies encountered by default Cellpose model in presence of large range size cells and provides information related to muscle Feret’s diameter distribution and Centrally Nucleated Fibers, thus depicting muscle health and treatment effects. MyoSOTHES achieves high quality segmentation compared to baseline workflow with a detection F1-score increasing from 0.801 to 0.919 and a Root Mean Square Error (RMSE) on diameter improved by 31%. MyoSOTHES was validated on an animal study featuring gene transfer in$$\gamma$$γ-Sarcoglycanopathy, for which dose-response effect is visible and conclusions drawn are consistent with those previously published. MyoSOTHES thus paves the way for wide quantification of HE stained muscle sections and retrospective analysis of HE labeled slices used in laboratories for decades.
Vaccination with a combination of planktonic and biofilm virulence factors confers protection against carbapenem-resistant Acinetobacter baumannii strains
Authors: Ramezanalizadeh Fatemeh , Rasooli Iraj , Owlia Parviz , Darvish Alipour Astaneh Shakiba , Abdolhamidi Raziyeh 
Published: November 2022

AbstractAcinetobacter baumannii is a multi-drug resistant pathogen with the ability to switch between planktonic and biofilm phenotypes. Although there is no vaccine against A. baumannii infections, many attempts have been made to develop vaccines using planktonic or biofilm antigens. To cover the different phenotypes of A. baumannii during growth and attachment, we combined planktonic upregulated antigens of iron receptors with biofilm upregulated antigens of pilus rods and evaluated immune responses and protective efficacies of the combined vaccine using lethal and sub-lethal murine sepsis models. The results showed that the combined vaccine elicited high IgG antibody titers and conferred protection against lethal doses of two Carbapenem-resistant high adherent A. baumannii strains. Complete bacterial clearance from all the affected tissues of the mice challenged with A. baumannii was an excellent achievement with our quadrivalent immunogen. These results demonstrate both planktonic and biofilm antigens are important during antigen selection for vaccine design.
An innovative scoring system for predicting an excellent Harris hip score after proximal femoral nail anti-rotation in elderly patients with intertrochanteric fracture
Authors: Phruetthiphat Ong-art , Pinijprapa Panukorn , Satravaha Yodhathai , Kitcharanant Nitchanant , Pongchaiyakul Chatlert 
Published: November 2022

AbstractTypically, intramedullary and extramedullary devices are used to treat elderly with intertrochanteric fractures. The majority of previous research has focused on the association between surgical factors and mechanical failure after internal fixation. There is, however, limited evidence to demonstrate the association between functional outcomes after proximal femoral nail anti-rotation (PFNA) fixation and the non-surgical factors such as patient’s comorbidities. The aim of this study is to determine the predictive factors associated with excellent outcome, as well as to develop an integrated scoring system to predict the outcome after PFNA fixation in elderly patients with an intertrochanteric fracture. A retrospective study was conducted between January 2012 and December 2018. Elderly patients with low-energy intertrochanteric fractures who underwent PFNA fixation and at least a year of follow-up were recruited. Demographics, comorbidities, cognitive status, time to operation, and surgical parameters of the patients were all identified. Excellent and non-excellent outcomes were assessed by Harris Hip Score (HHS) after a one-year follow up. Regression analysis was used to determine the predictors for an excellent functional outcome. A new integrated scoring system (ISSI; Integrate Scoring System in elderly patients with Intertrochanteric fracture) was developed and validated. 450 elderly patients were randomly divided into two cohorts: a development (N = 225) and validation cohorts (N = 225). In this study, age < 85 years, normal weight/overweight, Charlson comorbidity index (CCI) < 6, no cognitive impairment, a modified AO/OTA 31A1.3, time to operation < 6 days, and Tip Apex Distance between 20 and 30 mm were significantly associated with an excellent outcome after PFNA fixation. The range of ISSI score was between 0 to 16 and the cut-off score of 13 was found to have the highest discriminatory power to determine the excellent functional outcome where the area of ROC was 0.85. In regards to the validation cohort, the sensitivity and specificity of ISSI score was 69% and 87%, respectively, and the AUC was 0.81. The ISSI score is effortless and practical for orthopedic surgeons for predicting an outcome after PFNA fixation in elderly patients with an intertrochanteric fracture.