bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of gold nanoparticles-lateral flow test as a novel field diagnostic assay for detecting foot-and-mouth disease and lumpy skin disease viruses
Authors: Abdalhamed Abeer Mostafa , Naser Soad Mohammed , Mohamed Ayman Hamady , Gamil Zeedan Gamil Sayed 
Published: August 2022

Background and Objectives: Rapid diagnosis is a cornerstone for controlling and preventing viral disease outbreaks. The present study is aimed to develop a rapid field diagnostic test based on gold nanoparticles for the detection of lumpy skin diseases (LSD), and foot and mouth diseases (FMD) in animals with high sensitivity and specificity.
Materials and Methods: FMD and LSD vaccines were used as a source of viruses' antigens for preparing monoclonal anti- bodies and conjugated with gold nanoparticles that characterized using various techniques such as UV-visible spectrometry, and transmission electron microscopy (TEM). Monoclonal antibodies (mAbs) for each serotype produced in experimental rats and used to capture antibodies for FMDV and /or LSDV. ELISA was used to screen 469 milk samples and 1165 serum samples from naturally infected cattle, buffaloes, sheep, and goats for validation of the lateral flow test (LFT). LSDV DNA was extracted from 117 blood and skin biopsy samples collected from naturally infected cattle during the 2019 outbreak.
Results: The specificity and sensitivity of GNP-LFT were evaluated and compared to Ag-ELISA, Western blot tests (WB), and PCR. A total of 95 FMDV positives out of 469 (20.25%) milk samples and 268 FMDV positives out of 1165 (23.3%) serum samples from natural infected cattle, buffaloes, sheep, and goats examined by ELISA to valid GNPS-LFT Viral LSDV DNA was detected in 60/117 (51.5%) and 31/60 (52.9%). While the GNPS-LFT assay results were 49/117 (41.9%) and 29/60 (48.3%) blood and skin biopsy samples, respectively. The diagnostic sensitivity and specificity of the GNP-LFT test were 72% and 82%, respectively. All vesicular fluid and epithelium samples collected from infected animals were identified as positive by the GNP- LFT and Ag-ELISA. Ag-ELISA, on the other hand, was 90% and 100%. While the developed GNP- LFT used LSDV polyclonal antibodies were similar to ELISA and IgG-WB with a sensitivity of 72.8% and a specificity of 88.8%, respectively.
Conclusion: The GNPS-LFT is a novel immunoassay based on mono or polyclonal antibodies conjugated with gold nanopar- ticles that provides an accurate, rapid, specific, and sensitive tool for field rapid diagnosis of FMDV and LSDV.
Nasionalisme Dalam Puisi “Hanȋn” Karya Ahmad Rami dan Faruq Juwaidah (Sebuah Tinjauan Intertekstualitas)
Authors: Hasibuan Sammad 
Published: August 2022

Ahmad Rȃmi and Faruq Juwaidah are Arab writers of Egyptian nationality. Each of them made a work with the same title, namely the Hanȋn. The meaning that can be understood from the two poems is love for the homeland. So, this study will reveal the relationship between the two works of poetry, both in terms of similarities and differences. In this study, the author uses a descriptive-qualitative method, while the approach used is the intertextuality approach of Michael Riffaterre's model because the author also wants to find out which poems are hypograms and which are transformational works. Then, the results of data analysis are presented descriptively. The results showed that Rȃmi's Hanȋn poem is a hypogram of Faruq Juwaidah's Hanȋn poem. Both have similarities in terms of content or meaning, namely talking about love for the homeland. As for the difference, it can be seen from the choice of diction. To refer to Egypt as his homeland, Rȃmi used the term Ka'batu Amȃli, while Faruq chose the word qiblat al-'Usysyȃq. Later, Rȃmi's poems are very detailed and coherent, while Faruq's poetry is moredense and straight forward.

Asbtrak
Ahmad Rȃmi dan Faruq Juwaidah merupakan sastrawan Arab berkebangsaan Mesir. Masing-masing mereka membuat sebuah karya dengan judul yang sama, yaitu puisi Hanȋn. Makna yang dapat dipahami dari keduanya adalah cinta tanah air. Maka penelitian ini akan mengungkap hubungan kedua karya puisi tersebut, baik dari unsur persamaannya maupun perbedaannya. Dalam penelitian ini, penulis menggunakan metode deskriptif-kualitatif, sedangkan pendekatan yang dipakai adalah pendekatan intertekstualitas model Michael Riffaterre, sebab penulis juga ingin mencari puisi mana yang menjadi hipogram dan mana yang menjadi karya transformasi. Selanjutnya, hasil analisis data disajikan secara deskriptif. Hasil penelitian menunjukkan bahwa puisi Hanȋn karya Rȃmi merupakan hipogram dari puisi Hanȋn karya Faruq Juwaidah. Keduanya memiliki persamaan dalam hal isi atau kandungan makna, yaitu membicarakan cinta tanah Air. Adapun perbedaannya, terlihat dari pemilihan diksi. Untuk menyebut Mesir sebagai tanah air, Rȃmi menggunakan istilah Ka’batu Amȃli, sedangkan Faruq memilih kata qiblat al-‘Usysyȃq. Kemudian, puisi Rȃmi sangat rinci dan runut, sedangkan puisi Faruq lebih padat dan lugas.
The trend of temperature changes in Iranian metropolises in the last three decades
Authors: Lashkari Hassan , ghorlivand Nahid , Mohammadi Zainab , RouhbakhshSigaroodi HosseinAli 
Published: August 2022

Abstract
Global warming is a challenging issue among the world's climatologists, embraced even by politicians and the common people. In this study, the trend of temperature changes in 31 metropolises of Iran, which are generally the provincial centres of the country, has been studied in a statistical period of 35 years (1985-2019). We found that there are more temperature changes in all metropolises of Iran in winter than other seasons. In this season, the maximum temperature parameter shows a significant increase, as evidenced in Ardabil with about 9 degrees Celsius. Also, the highest amount of temperature increase in all seasons has occurred in the western and northwestern part of Iran, being of mildly cold to extremely cold climate in Iran. In general, the temperature changes more intensively from the south to the north regions. It is interesting that in the southern and coastal cities of Iran, such as Ilam, Bushehr and Shiraz, in some months, the temperature keeps decreasing. The increase in temperature of metropolises has been much more regular for the maximum temperature than the minimum temperature. Strangely, unlike all the months in November, the maximum temperature has decreased in most cities in Iran.
The role of the California tier system in controlling population mobility during the COVID- 19 pandemic
Authors: Schwarz Emilie , Schwarz Lara , Teyton Anaïs , Crist Katie , Benmarhnia Tarik 
Published: August 2022

Abstract
Background
Policies to restrict population mobility are a commonly used strategy to limit the transmission of contagious diseases. Among measures implemented during the COVID-19 pandemic were dynamic stay-at-home orders informed by real-time, regional-level data. California was the only state in the U.S. to implement this novel approach; however, the effectiveness of California’s four-tier system on population mobility has not been quantified.
Methods
Utilizing data from mobile devices and county-level demographic data, we evaluated the impact of policy changes on population mobility and explored whether demographic characteristics explained variability in responsiveness to policy changes. For each California county, we calculated the proportion of people staying home and the average number of daily trips taken per 100 persons, across different trip distances and compared this to pre-COVID-19 levels.
Results
We found that overall mobility decreased when counties moved to a more restrictive tier and increased when moving to a less restrictive tier, as the policy intended. When placed in a more restrictive tier, the greatest decrease in mobility was observed for shorter and medium-range trips, while there was an unexpected increase in the longer trips. The mobility response varied by geographic region, as well as county-level median income, gross domestic product, economic, social, and educational contexts, the prevalence of farms, and recent election results.
Conclusions
This analysis provides evidence of the effectiveness of the tier-based system in decreasing overall population mobility to ultimately reduce COVID-19 transmission. Results demonstrate that socio-political demographic indicators drive important variability in such patterns across counties.
Preparedness of medical students to provide nutrition care following a nutrition education intervention
Authors: Amoore Bright Yammaha , Gaa Patience Kanyiri , Ziblim Shamsu-Deen , Mogre Victor 
Published: August 2022

Abstract
Objective: We assessed the effects of a nutrition education intervention on medical students’ self-perceived preparedness to provide nutrition care. We administered a pre, post and four-weeks follow-up questionnaire to second year medical school who participated in a nutrition education intervention that adapted varied teaching and learning activities. Outcomes were self-perceived preparedness, relevance of nutrition education and perceived need for further training in nutrition.Results: The proportion of participants who felt prepared to provide nutrition care increased significantly (p= 0.001) from 38% (n = 35) at baseline to 65.2% (n = 60) immediately post-intervention and to 63.2% (n = 54) at 4-weeks follow-up. At baseline, 74.2% (n = 69) of the students perceived nutrition education to be relevant to their future career as medical doctors which increased to 85% (n = 78) immediately after the intervention (p= 0.026) and to 76% (n = 70) 4-weeks follow-up. The proportion of participants who reportedly said they will benefit from further training in nutrition increased from 63.8% (n = 58) at pre-intervention to 74.0% (n = 68) at post-intervention (p= 0.016).Conclusion: An innovative, multiple-strategy nutrition education intervention can improve medical students’ self-perceived preparedness to provide nutrition care.
Effect of chronic exposure to fine particulate matter on cardiac tissue of NZBWF1 mice
Authors: Waked Dunia , Rodrigues Ana Clara Bastos , Silva Thamires Moraes , Yariwake Victor Yuji , Veras Mariana Matera 
Published: August 2022

Abstract
Epidemiological and toxicological studies have shown that inhalation of particulate matter (PM) is associated with development of cardiovascular diseases. Long-term exposure to PM may increase the risk of cardiovascular events and reduce life expectancy. Systemic lupus erythematosus (SLE) is a chronic inflammatory disease with autoimmune nature, characterized by the production of autoantibodies that affects several organs, including the heart. Caused by several factors, air pollution, may appear as one of the most important points on the onset and development of SLE. This study aims to investigate whether exposure to air pollution promotes increased inflammation and cardiac remodeling in animals predisposed to SLE. Female mice of NZBWF1, were used in an environmental particle concentrator. Aspects related to cardiac remodeling, inflammation and apoptosis in myocardium were analyzed. Body weight gain, cardiac trophism by heart/body weight ratio, relative area of cardiomyocytes and fibrotic area of cardiac tissue were evaluated during exposure period. Animals exposed to PM2.5 showed increased area of cardiomyocytes, and area of fibrosis, in addition, we observed an increase of IL-1 and C3 in cardiac tissue, demonstrating increased inflammation. We suggest that air pollution is capable of promoting cardiac remodeling and increased inflammation in animals predisposed to SLE.
Theoretical study of impedance to consider the effects of electrode area
Authors: Sung Heehun 
Published: August 2022

Abstract
Electrical impedance tomography is one of the most popular methods to investigate subsurface resistivity and structure; however, it does not take into account the effect of the electrode area because it is based on methods for measuring the resistance of large-scale objects like the Earth. The purpose of the present study is to propose a new theoretical electrical impedance equation to obtain reliable electrical resistance measurements in tests of human-scale objects. The finite element method is applied to investigate how the impedance of a model to which electrodes are to be attached changes depending on the area of the electrodes. Both the electrodes and the model are constructed and calculated through COMSOL. The electrical impedance is simulated according to the area of the electrode, the length of the model, the conductivity, and the frequency of the electric field. On the basis of the simulation results according to the geometric structure of electrodes and the model, a new electric impedance equation for the electrode area is formulated. Unless the shape of the model is a circular plate or a long column, the results fit well with the theoretical calculations. Also, if the model height is similar to the model length, the theoretical impedance can only be defined as an arctangent function. This equation is important for analyzing the effect of the electrode area and is effective for estimating the resistance of human-scale models.
CCT2 enhances the proliferation and migration of head and neck squamous cell carcinoma via regulating cell cycle
Authors: Lu Lin , Li Xuerong , Zhao Yiwei , Zhai Xuefan , Cai Min , Bao Baicheng , Li Menghuan , Sun Jianbo 
Published: August 2022

Abstract
Recent therapeutic advances have improved the survival of head and neck squamous cell carcinoma (HNSCC) patients, but the prognosis of HNSCC remains dismal. Further understanding of the underlying mechanism of HNSCC progression is still an urgent need. In this study, bioinformatics-based analysis revealed that Chaperonin containing TCP-1, subunit 2 (CCT2) is significantly upregulated in HNSCC and related to pathoclinical outcomes, which is validated by the fact that four human HNSCC cell lines all express higher CCT2 than the normal oral squamous cell line. Clinically, high CCT2 expression was positively associated with worse overall survival (OS) and TNM classification in HNSCC patients. Further study indicated that suppression of CCT2 by RNA interference significantly inhibits cell proliferation, migration and invasion, arrests cell cycle at G2 phase, and prevents epithelial-mesenchymal transition (EMT) of both SAS and HSC-3 cell lines. In vivo assays further verified that CCT2 knockdown inhibited tumor growth in HNSCC. Moreover, knockdown of CCT2 led to the significant decrease of Cdc20 as well as cyclin D1, cyclin E1, and CDK6 while increase of GSK3-α/β and p27, which shed light into molecular mechanism of CCT2 function. In conclusion, elevated CCT2 expression promotes HNSCC cell proliferation via Cdc20 mediated cyclin-CDK pathway and CCT2 would be a valuable prognostic biomarker and therapeutic target in HNSCC.
Clinician and computer: a study on doctors’ perceptions of artificial intelligence in skeletal radiography
Authors: York Thomas , Raj Siddarth , Ashdown Thomas , Jones Gareth 
Published: August 2022

Abstract
Background - Traumatic musculoskeletal injuries are a common presentation to emergency care, the first-line investigation often being plain radiography. The interpretation of this imaging frequently falls to less experienced clinicians despite well-established challenges in reporting. This study presents novel data of clinicians’ confidence in interpreting trauma radiographs, their perception of AI in healthcare, and their support for the development of systems applied to skeletal radiography.Methods- A novel questionnaire was distributed through a network of collaborators to clinicians across the Southeast of England. Over a three-month period, responses were compiled into a database before undergoing statistical review. Results- The responses of 297 participants were included. The mean self-assessed knowledge of AI in healthcare was 3.68 out of ten, with significantly higher knowledge reported by the most senior doctors (ST3/SpR or above=4.88). 13.8% of participants reported an awareness of AI in their clinical practice. Overall, participants indicated substantial favourability towards AI in healthcare (7.87) and in AI applied to skeletal radiography (7.75). There was a preference for a hypothetical system indicating positive findings rather than ruling as negative (7.26 vs 6.20).Conclusions - This study identifies clear support, amongst a cross section of student and qualified doctors, for both the general use of AI technology in healthcare and in its application to skeletal radiography for trauma. The development of systems to address this demand appear well founded and popular. The engagement of a small but reticent minority should be sought, along with improving the wider education of doctors on AI.
H∞ controller design for the vibration suppression of the rolling mill system with time-varying input delay
Authors: Qian Cheng , Zhang Liuliu , Hua Changchun 
Published: August 2022

Abstract
The rolling mill system has the characteristic of measurement delay. In order to deal with the effects of time-delayed feedback from strip thickness measurement on vibration suppression, this paper studies the vibration suppression control strategy for the rolling mill system under time varying delay. Initially, the nonlinear rolling force and disturbance are taken into consideration during the dynamic rolling process. The mechanism-hydraulic coupling vibration model is established based on the flow equation and Newton's second law. Meanwhile, input delay caused by strip thickness measurement is considered in the rolling mill system and a time-delayed coupling vibration state-space model is introduced via choosing the appropriate state variables. Secondly, the corresponding linear matrix inequalities (LMIs) are derived from an augmented Lyapunov-Krasovskii functional (LKF) with advanced inequalities and are used to synthesize a controller that can achieve the asymptotical stability and H ∞ performance index. Finally, the effectiveness of the proposed control algorithm is verified by simulation experiment results.
Collagen Ⅰ-DDR1 signaling promotes hepatocellular carcinoma cell stemness via Hippo signaling repression.
Authors: Xiong Yi-xiao , Zhang Xiaochao , Zhu Jing-han , Zhang Yuxin , Pan Yonglong , Wu Yu , Zhao Jian-ping , Liu Junjie , Lu Yuan-xiang , liang huifang , Zhang Zhanguo , Zhang Wanguang 
Published: August 2022

Abstract
Cancer stem cells (CSCs) are a minority population of cancer cells with stemness and multiple differentiation potentials, leading to cancer progression and therapeutic resistance. However, the concrete mechanism of CSCs in hepatocellular carcinoma (HCC) remains obscure. We found that in advanced HCC tissues, collagen Ⅰ was upregulated, which is consistent with the expression of its receptor DDR1. Accordingly, high collagen Ⅰ levels accompanied by high DDR1 expression are associated with a poor prognosis in patients with HCC. Collagen Ⅰ-induced DDR1 activation enhanced HCC cell stemness in vitro and in vivo. Mechanistically, DDR1 interacts with CD44, which acts as a co-receptor that amplifies collagen Ⅰ-induced DDR1 signaling, and collagen Ⅰ-DDR1 signaling antagonized Hippo signaling by facilitating the recruitment of PP2AA to MST1, leading to exaggerated YAP activation. The combined inhibition of DDR1 and YAP synergistically abrogated HCC cell stemness in vitro and tumorigenesis in vivo. A radiomic model based on T2 weighted image can noninvasively predict collagen Ⅰ expression. These findings reveal the molecular basis of collagen Ⅰ-DDR1 signaling inhibiting Hippo signaling and highlight the role of CD44/DDR1/YAP axis in promoting cancer cell stemness, suggesting that DDR1 and YAP may serve as novel prognostic biomarkers and therapeutic targets in HCC.
USP39-mediated deubiquitination of Cyclin B1 at Lys242 promotes tumor cell cycle G2/M transition and glioma progression
Authors: chen Weilin , Xiao Yue , Chen Xinyi , Hu Weiwei , Ma Wenjing , Di Qianqian , Tang Haimei , Zhao Xibao , Huang Guodong 
Published: August 2022

Abstract
The elevated Cyclin B1 expression contributes to various tumorigenesis and poor prognosis. Cyclin B1 expression could be regulated by ubiquitination and deubiquitination. However, the mechanism of how Cyclin B1 is deubiquitinated and its roles in human glioma remain unclear. In this study, co-immunoprecipitation and other assays were performed to detect the interacting of Cyclin B1 and USP39. A series of in vitro and in vivo experiments were performed to investigate the effect of USP39 on the tumorigenicity of tumor cells. We discover that USP39 interacts with Cyclin B1 and stabilizes its expression by deubiquitinating Cyclin B1. Notably, we demonstrate that USP39 cleaves the K29-linked polyubiquitin chain on Cyclin B1 at Lys242. Additionally, overexpression of Cyclin B1 rescues the arrested cell cycle at G2/M transition and the suppressed proliferation of glioma cells caused by USP39 knockdown in vitro. Furthermore, USP39 promotes the growth of glioma xenograft in subcutaneous and in situ of nude mice. Finally, in human glioma and other tumor specimens, the expression levels of USP39 and Cyclin B1 proteins are clinically positively relevant. In summary, our data support the evidence that USP39 acts a novel deubiquitinating enzyme of Cyclin B1 and promoted tumor cell proliferation at least in part through Cyclin B1 stabilization, represents a promising therapeutic strategy for tumor patients.
On the p-Renyi entropy power for nonlinear diffusion equations on Riemannian manifolds
Authors: Wang Yu-Zhao , Zhang Hui-Ting 
Published: August 2022

Abstract
In this paper, we use a new method to prove the concavity of p-Rényi entropy power for doubly nonlinear diffusion equations on compact Riemanian manifolds with nonnegative Ricci curvature based on an explicit variational formula via trace free tensors. Moreover, we obtain the NIW formula between p-Renyi entropy power, p-Fisher information and W-entropy for doubly nonlinear diffusion equations.Mathematics Subject Classification (2010)﻿. Primary 58J35; Secondary 35K55.
GCMS based metabolite profiling of first flush Darjeeling tea (orthodox) through the processing steps: First report.
Authors: De Dwaipee , Sarkar Sreerupa , Chhetri Himashree , Chatterjee Jhelam , Sinha Nitu , Das Sujit , Sarkar Abhijit , Das Susmita , Ray Sonali 
Published: August 2022

Abstract
Climatic and soil conditions affect the first flush metabolite composition of the economic crop Darjeeling tea, can impact flavour, nutrition and overall consumer preferences. We sampled Camellia sinensis var. sinensis grown in Darjeeling hills and from different processing steps.75 metabolites have been illustrated utilizing GC/MS based metabolomic and chemometric tool. Metabolomic profiling performed across production will aim identification of quality markers and establishing database applicable for Darjeeling tea authentication, the first GI product of India. PCA and PLSDA show differences in tea metabolites through the processing steps. The chemometric analysis elucidates the changes in important metabolites. The antioxidant activity showed significant changes. The amino acids enhanced significantly (p < 0.05), from fresh tea leaves to finally processed product. The organic acids, inorganic acids and fatty acid compounds decreased. Among the phenolic compounds, caffeic acids, epicatechin, epigallocatechin, chlorogenic acid, 3, 4-dihydroxybenzoic acid and 4-hydroxycinnamic acid showed increase in their concentration, whereas catechin, (-) - epicatechin, gallic acid, pyrogallol, quinic acid, shikimic acid, arbutin showed remarkable decrease in concentration in finally processed tea. The flavonoids kaempferol and taxifolin showed significant decrease in their concentration.During first flush meteorological parameters recorded along with variation of PM2.5, PM10, O3, NO2, and SO2 concentrations and AQI.