bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photopic negative response recorded with RETeval system in eyes with optic nerve disorders
Authors: Yamashita Tsutomu , Kato Kumiko , Kondo Mineo , Miki Atsushi , Araki Syunsuke , Goto Katsutoshi , Ieki Yoshiaki , Kiryu Junichi 
Published: May 2022

AbstractElectroretinography (ERG) is used to evaluate the physiological status of the retina and optic nerve. The purpose of this study was to determine the usefulness of ERGs recorded with the RETeval system in diagnosing optic nerve diseases. Forty-eight patients with optic nerve disorders, including optic neuritis, ischemic optic neuropathy, traumatic optic neuropathy, and dominant optic atrophy, and 36 normal control subjects were studied. The amplitudes of the photopic negative response (PhNR) were recorded with the RETeval system without mydriasis. The circumpapillary retinal nerve fiber layer thickness (cpRNFLT) was determined by optical coherence tomography (OCT). The significance of the correlations between the PhNR and cpRNFLT parameters were determined, and the receiver operating curve (ROC) analyses were performed for the PhNR and cpRNFLT. Patients with optic nerve disorders had significantly smaller PhNRs compared to the control subjects (P = 0.001). The ROC analyses indicated that both PhNR and cpRNFLT had comparable diagnostic abilities of detecting optic nerve disorders with PhNR at 0.857 and cpRNFLT at 0.764. The PhNR components recorded with the RETeval system have comparable diagnostic abilities as the cpRNFLT in diagnosing optic nerve disorders.
Angiographic characteristics of vasculopathy in patients with idiopathic inflammatory myopathies and systemic sclerosis
Authors: Yeo Jina , Park Eun-Ah , Lee Eun Bong , Park Jin Kyun 
Published: May 2022

AbstractTo describe the peripheral angiographic features of vasculopathy in idiopathic inflammatory myopathies (IIM) and systemic sclerosis (SSc) in comparison to polyarteritis nodosa (PAN). Angiograms of 47 extremities (24 upper and 23 lower) of 11 patients with IIM (n = 5) and SSc (n = 6), and 12 patients with PAN who presented with critical limb ischemia were retrospectively analyzed with regards to the presence of stenosis, occlusion, aneurysms and delayed distal flow, and degree of neovascularization. Diffuse narrowing was more frequent (66.1 vs. 38.0%, p = 0.001), whereas multifocal stenosis (6.5% vs. 26.8%, p = 0.002), abrupt occlusion (11.3% vs. 29.6%, p = 0.010) and aneurysm formation (1.6% vs. 11.3%, p = 0.037) were less frequent in IIM/SSc than PAN. In distal arteries, tapered occlusion (95.5% vs. 76.0%, p = ns) and delayed flow (77.3% vs. 48.0%, p = 0.039) were more common in IIM/SSc than PAN. After 1 year, auto- or surgical amputation tended to be more frequent in IIM/SSc than PAN (36.4% vs. 16.7%, p = ns). In conclusion, diffuse narrowing, tapered occlusion and delayed distal flow on conventional angiograms tend to be more frequent in IIM/SSc than PAN. Further studies are needed to verify these findings in a larger prospective cohort.
Pharmacologic modulation of 5-fluorouracil by folinic acid and pyridoxine for treatment of patients with advanced breast carcinoma
Authors: Machover David , Goldschmidt Emma , Almohamad Wathek , Castagné Vincent , Dairou Julien , Desterke Christophe , Gomez Léa , Gaston-Mathé Yann , Boucheix Claude 
Published: May 2022

AbstractHigh concentration pyridoxal 5’-phosphate, the cofactor of vitamin B6, potentiates cytotoxicity in cancer cells exposed to 5-fluorouracil (FUra) and folinic acid (FA). We studied the effect of high-dose pyridoxine on antitumor activity of regimens comprising FUra and FA in 27 advanced breast carcinoma patients. Of 18 previously untreated patients, 12 had tumors that did not overexpress HER2 (Group I), and 6 that overexpressed HER2 (Group II). Nine patients (Group III) had prior chemotherapy. Group I received AVCF (doxorubicin, vinorelbine, cyclophosphamide, FUra, FA) or FAC (doxorubicin, cyclophosphamide, FUra, FA) followed by TCbF (paclitaxel carboplatin, FUra, FA). Groups II, and III received TCbF. Pyridoxine iv (1000–3000 mg/day) preceded each FA and FUra. Group II also received trastuzumab and pertuzumab. 26 patients responded. Three patients in Group I had CRs and 9 had PRs with 62–98% reduction rates; 4 patients in Group II had CRs and 2 had PRs with 98% reduction. Of 7 measurable patients in Group III, 2 attained CRs, and 5 had PRs with 81–94% reduction rates. Median time to response was 3.4 months. Unexpected toxicity did not occur. This pilot study suggests that high-dose vitamin B6 enhances antitumor potency of regimens comprising FUra and FA.
Highly sensitive and selective detection of dopamine with boron and sulfur co-doped graphene quantum dots
Authors: Chatterjee Manisha , Nath Prathul , Kadian Sachin , Kumar Anshu , Kumar Vishal , Roy Partha , Manik Gaurav , Satapathi Soumitra 
Published: May 2022

AbstractIn this work, we report, the synthesis of Boron and Sulfur co-doped graphene quantum dots (BS-GQDs) and its applicability as a label-free fluorescence sensing probe for the highly sensitive and selective detection of dopamine (DA). Upon addition of DA, the fluorescence intensity of BS-GQDs were effectively quenched over a wide concentration range of DA (0–340 μM) with an ultra-low detection limit of 3.6 μM. The quenching mechanism involved photoinduced electron transfer process from BS-GQDs to dopamine-quinone, produced by the oxidization of DA under alkaline conditions. The proposed sensing mechanism was probed using a detailed study of UV–Vis absorbance, steady state and time resolved fluorescence spectroscopy. The high selectivity of the fluorescent sensor towards DA is established. Our study opens up the possibility of designing a low-cost biosensor which will be suitable for detecting DA in real samples.
Urinary metabolic modulation in human participants residing in Siachen: a 1H NMR metabolomics approach
Authors: Gandhi Sonia , Chinnadurai Vijayakumar , Bhadra Kuntal , Gupta Isha , Kanwar Ratnesh Singh 
Published: May 2022

AbstractThe main physiological challenge in high altitude environment is hypoxia which affects the aerobic metabolism reducing the energy supply. These changes may further progress toward extreme environment-related diseases. These are further reflected in changes in small molecular weight metabolites and metabolic pathways. In the present study, metabolic changes due to chronic environmental hypoxia were assessed using 1H NMR metabolomics by analysing the urinary metabolic profile of 70 people at sea level and 40 people at Siachen camp (3700 m) for 1 year. Multivariate statistical analysis was carried out, and PLSDA detected 15 metabolites based on VIP score > 1. ROC analysis detected cis-aconitate, Nicotinamide Mononucleotide, Tyrosine, Choline and Creatinine metabolites with a high range of sensitivity and specificity. Pathway analysis revealed 16 pathways impact > 0.05, and phenylalanine tyrosine and tryptophan biosynthesis was the most prominent altered pathway indicating metabolic remodelling to meet the energy requirements. TCA cycle, Glycine serine and Threonine metabolism, Glutathione metabolism and Cysteine alterations were other metabolic pathways affected during long-term high-altitude hypoxia exposure. Present findings will help unlock a new dimension for the potential application of NMR metabolomics to address extreme environment-related health problems, early detection and developing strategies to combat high altitude hypoxia.
Bifunctional CePO4/CeO2 nanocomposite as a promising heterogeneous catalyst for the enhancement of the ozonation recovery effect in the presence of chloride ions
Authors: Fijołek Lilla , Wolski Lukasz 
Published: May 2022

AbstractThe degradation of organics through ozonation is strongly reduced by chloride ions. Although the efficiency of such processes can be recovered in the presence of homogeneous phosphates, the addition of these chemicals to water is problematic because of the generation of secondary wastes. Phosphates are known as one of the most important biogens responsible for the eutrophication of rivers and lakes. Thus, their worldwide application should be limited. The main goal of this work was to characterize the performance of solid-state cerium(III) phosphate (CePO4), cerium dioxide (CeO2), and bifunctional CePO4/CeO2 nanocomposite as substitutes for homogeneous phosphates during the ozonation of benzoic acid (BA) in the presence of chlorides. All solid-state samples used in this study were synthesized by facile hydrothermal method and thoroughly characterized. It was documented that heterogeneous CePO4 showed significantly better ozonation recovery effect than homogeneous phosphates. It was also established that the process efficiency could be further enhanced by using the bifunctional nanocomposite. Tests with the use of tert-butanol as a hydroxyl radical scavenger revealed that the improved ozonation efficiency in the presence of CePO4/CeO2 resulted from the action of HO• radicals which were the key reactive oxygen species responsible for the recovery of BA degradation in the presence of chlorides.
Islet cell replacement and transplantation immunology in a mouse strain with inducible diabetes
Authors: Bhagchandani Preksha , Chang Charles A. , Zhao Weichen , Ghila Luiza , Herrera Pedro L. , Chera Simona , Kim Seung K. 
Published: May 2022

AbstractImproved models of experimental diabetes are needed to develop cell therapies for diabetes. Here, we introduce the B6 RIP-DTR mouse, a model of experimental diabetes in fully immunocompetent animals. These inbred mice harbor the H2bmajor histocompatibility complex (MHC), selectively express high affinity human diphtheria toxin receptor (DTR) in islet β-cells, and are homozygous for thePtprca(CD45.1) allele rather than wild-typePtprcb(CD45.2). 100% of B6 RIP-DTR mice rapidly became diabetic after a single dose of diphtheria toxin, and this was reversed indefinitely after transplantation with islets from congenic C57BL/6 mice. By contrast, MHC-mismatched islets were rapidly rejected, and this allotransplant response was readily monitored via blood glucose and graft histology. In peripheral blood of B6 RIP-DTR with mixed hematopoietic chimerism, CD45.2 BALB/c donor blood immune cells were readily distinguished from host CD45.1 cells by flow cytometry. Reliable diabetes induction and other properties in B6 RIP-DTR mice provide an important new tool to advance transplant-based studies of islet replacement and immunomodulation to treat diabetes.
How the metabolic phenotype in adulthood is affected by long-lasting immunological trajectories since adolescence
Authors: Barroso Isaac , Guimarães João Tiago , Craveiro Vanda , Severo Milton , Ramos Elisabete 
Published: May 2022

AbstractA close relationship between immune and metabolic systems has been perceived in the recent past. We aimed to assess whether the immunological trajectories of circulating white blood cells (WBC) started in adolescence, affects the metabolic phenotype in adulthood. We used data from 1183 participants of the population-based EPITeen cohort, evaluated at 13, 17, 21, 24 and 27 years of age. The Immunological trajectories from 13 to 27 years old were identified by mixed-effects models, being their association with metabolic features at 27 years old measured by logistic regression. The Higher Inflammatory Activation trajectory (HIA trajectory) had the highest percentage of individuals with metabolic syndrome, while Lowest Levels of WBC trajectory (LLWBC trajectory) showed the lowest percentage. Participants with HIA trajectory had significantly higher triglycerides, waist circumference, serum uric acid and BMI. After adjustment for sex and sports practice and hs-CRP, the odds of having one or more metabolic features in adulthood was significantly lower in LLWBC trajectory. Individuals with immunological trajectories of WBC linked with a pattern of higher immune activation showed a less favorable metabolic profile, while those with the lowest levels of WBC were less likely to have metabolic risk factors in adulthood.
Clinical findings and predictive factors for positive anti-interferon-γ autoantibodies in patients suffering from a non-tuberculosis mycobacteria or Talaromyces marneffei infection: a multicenter prospective cohort study
Authors: Qiu Ye , Tang Mengxin , Zeng Wen , Feng Xin , Pan Mianluan , Li Wei , Zhang Jianquan 
Published: May 2022

AbstractWe investigated the clinical features and screened for predictive factors of anti-interferon-γ autoantibody (AIGA) positivity. We enrolled 63 AIGA-positive (group 1) and 29 AIGA-negative (group 2) HIV-negative patients. White blood cell (WBC) and neutrophil counts, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP), globulin, immunoglobulin (Ig) G, and IgM levels were higher, whereas CD4+T cell count and hemoglobin level were lower in group 1 than in group 2. Co-infections, multiple infections, and disseminated infections were significantly higher in group 1 than in group 2. Prognosis was worse in group 1 than in group 2, especially for relapse and persistent infections. The number of infecting pathogens and sites involved; WBC and neutrophil counts; globulin, IgG, IgM, and CRP levels; and ESR were significantly positively correlated with AIGA titers; however, CD4+T cell count was significantly negatively correlated with AIGA titers. Therefore, IgG, globulin, and CRP levels; CD4+T cell and WBC counts; the number of infecting pathogens and sites involved; and ESR were considered potential predictors for AIGA positivity. For HIV-negative hosts with double or multiple opportunistic, disseminated infections and high serum IgG and globulin levels, low CD4+T cell count, and an increase in inflammatory marker levels, positive AIGA-associated immunodeficiency should be considered.
Substantiation of propitious “Enzybiotic” from two novel bacteriophages isolated from a wastewater treatment plant in Qatar
Authors: Ramadoss Ramya , Al-Shukri Moza , Shomar Basem , Ilyin Valentin Alekseevich , Vincent Annette Shoba 
Published: May 2022

AbstractLysin of bacteriophages isolated from a particular ecosystem could be inducted as a bio-controlling tool against the inhabiting pathogenic bacterial strains. Our study aims at both experimental and computational characterization of the identical lysin gene product inherent in the genomes of two novel Myoviridae bacteriophages, Escherichia Phage C600M2 (GenBank accession number OK040807, Protein ID: UCJ01465) and Escherichia Phage CL1 (GenBank Genome accession number OK040806.1, Protein ID: UCJ01321) isolated from wastewater collected from the main water treatment plant in Qatar. The lysin protein, evinced to be a globular N-acetyl-muramidase with intrinsic “cd00737: endolysin_autolysin” domain, was further expressed and purified to be experimentally validated by turbidimetric assay for its utility as an anti-bacterial agent. Comprehensive computational analysis revealed that the scrutinized lysin protein shared 85–98% sequence identity with 61 bacteriophages, all native to wastewater allied environments. Despite varied Host Recognition Components encoded in their genomes, the similitude of lysins, suggests its apparent significance in host–pathogen interactions endemic to wastewater environment. The present study substantiates the identical lysin from Escherichia Phage C600M2 and Escherichia Phage CL1 as propitious “enzybiotic”, a hybrid term to describe enzymes analogous to anti-biotics to combat antibiotic-resistant bacteria by in silico analysis and subsequent experimental validation.
Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts
Authors: Moreau Marielle , Capallere Christophe , Chavatte Laurent , Plaza Christelle , Meyrignac Céline , Pays Karl , Bavouzet Bruno , Botto Jean-Marie , Nizard Carine , Bulteau Anne-Laure 
Published: May 2022

AbstractReconstructed human epidermis equivalents (RHE) have been developed as a clinical skin substitute and as the replacement for animal testing in both research and industry. KiPS, or keratinocytes derived from induced pluripotent stem cells (iPSCs) are frequently used to generate RHE. In this study, we focus on the mitochondrial performance of the KiPS derived from iPSCs obtained from two donors. We found that the KiPS derived from the older donor have more defective mitochondria. Treatment of these KiPS with a plant extract enriched in compounds known to protect mitochondria improved mitochondrial respiration and rendered them fully competent to derive high-quality RHE. Overall, our results suggest that improving mitochondrial function in KiPS is one of the key aspects to obtain a functional RHE and that our plant extracts can improve in this process.
A new versatile MR-guided high-intensity focused ultrasound (HIFU) device for the treatment of musculoskeletal tumors
Authors: Cabras Paolo , Auloge Pierre , Bing Fabrice , Rao Pramod Prabhakar , Hoarau Stéphanie , Dumont Erik , Durand Alexandre , Maurin Benjamin , Wach Benoit , Cuvillon Loïc , Breton Elodie , Gangi Afshin , Vappou Jonathan 
Published: May 2022

AbstractMagnetic Resonance (MR) Imaging-guided High Intensity focused Ultrasound (MRgHIFU) is a non-invasive, non-ionizing thermal ablation therapy that is particularly interesting for the palliative or curative treatment of musculoskeletal tumors. We introduce a new modular MRgHIFU device that allows the ultrasound transducer to be positioned precisely and interactively over the body part to be treated. A flexible, MR-compatible supporting structure allows free positioning of the transducer under MRI/optical fusion imaging guidance. The same structure can be rigidified using pneumatic depression, holding the transducer rigidly in place. Targeting accuracy was first evaluated in vitro. The average targeting error of the complete process was found to be equal to 5.4 ± 2.2 mm in terms of focus position, and 4.7° ± 2° in terms of transducer orientation. First-in-man feasibility is demonstrated on a patient suffering from important, uncontrolled pain from a bone metastasis located in the forearm. The 81 × 47 × 34 mm3 lesion was successfully treated using five successive positions of the transducer, under real-time monitoring by MR Thermometry. Significant pain palliation was observed 3 days after the intervention. The system described and characterized in this study is a particularly interesting modular, low-cost MRgHIFU device for musculoskeletal tumor therapy.
Reading Warm-Up, Reading Skill, and Reading Prosody When Reading the My Grandfather Passage: An Exploratory Study Born Out of the Motor Planning Theory of Prosody and Reading Prosody Research
Authors: Boutsen Frank , Park Eunsun , Dvorak Justin D. 
Published: May 2022

Purpose:The Motor Planning Theory of Prosody and reading prosody research indicate that “out of the blue” oral reading, as practiced in clinical and research settings, invokes surface rather than covert prosody, particularly when readers are recorded, less skilled, and/or speech impaired. Warm-up is not considered in passage reading for motor-speech assessment. We report on a preliminary study aimed to investigate the effect of warm-up on reading prosody in two conditions: silent reading alone and reading “out of the blue” followed by silent reading. A secondary aim of the study was to examine the effect of reading skill on reading prosody.Method:Twenty-one monolingual, English-speaking volunteers were recorded reading the My Grandfather Passage (GP) while their eye movements were tracked. Participants were randomly assigned to one of two reading conditions: (a) silent–oral (SO) and (b) oral–silent–oral (OSO). In the SO condition, participants read the GP silently as a warm-up for the subsequent oral reading. In the OSO condition, participants first read the GP aloud (“out of the blue”) and then read the same passage silently with the instruction to do this in preparation for a second oral reading. Reading skill was quantified using eye–voice span and Wide Range Achievement Test–Fourth Edition testing. Reading prosody was evaluated using pause indexes, the Acoustic Multidimensional Prosody Index, and speech rate.Conclusions:One oral reading before a silent reading but not a silent reading alone before oral reading was shown to affect reading prosody. In terms of reading skill, results indicate that predictive associations patterned differently in the reading conditions explored, suggesting different underlying skill sets.
Multicasting in NOMA‐based UAV networks: Path design and throughput maximisation
Authors: Salar Hosseini Shima , Javan Mohammad Reza , Nazari Ali 
Published: May 2022

AbstractIn this paper, a new resource allocation framework for unmanned aerial vehicle (UAV) assisted multicast wireless networks is proposed in which the network users according to their request are divided into several multicast groups. Power domain non‐orthogonal multiple access is adopted as the transmission technology using which the dedicated signals of multicast groups are superimposed and transmitted simultaneously as the UAV passes over the communication area for fixed and mobile users. The proposed scenarios are discussed from two perspectives, offline and online modes. In offline mode, the problem is implemented for fixed and mobile users whose locations are predictable (the location of users, over the communication time, is known at the beginning of the communication time) and in online mode for mobile users whose locations are unpredictable (the location of users, over the communication time, is unknown at the beginning of the communication time). Also, a scenario is proposed in which the online mode the number of mobile users can grow in each time slot. The problem of joint power allocation and UAV trajectory design as an optimisation problem that is non‐linear and non‐convex for two proposed scenarios is formulated. To solve the problem, an alternate search method, successive convex approximation, and geometric programming are adopted. Using simulation results, the performance of the proposed scheme is evaluated for different values of the network parameters.
Analytical modelling for magnetic field of interior permanent magnet synchronous motors accounting for bridge saturation
Authors: Wang Huimin , Sun Zihan , Zhong Saichao , Yan Yan , Wu Shuang , Guo Liyan 
Published: May 2022

AbstractMost of the motors used in industrial fields are interior permanent magnet synchronous motors (IPMSMs). The rotor structure of IPMSM is complex, and the rotor bridge region is affected by non‐linear saturation, which increases the difficulty of modelling and calculation. The accuracy of traditional methods for the magnetic field prediction needs to be improved. An analytical model for IPMSM that accounts for the bridge saturation and the complex rotor structure is presented. This method transformed the structure of IPMSM equivalently, which makes it easier to use modelling in the polar coordinate system. Meanwhile, the bridge subdomain and the bridge saturation overflow subdomain are established separately based on the different saturation levels, and the relative permeability value of each subdomain is solved by the iterative permeability method. The magnetic field and electromagnetic performance obtained using the analytical method are compared with those of the finite element method. Finally, the analytical predictions are compared with the measured data to confirm the validity of the methods proposed.