bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of Home Medication Storage Location in Increasing Medication Adherence for Older Adults
Authors: Gualtieri Lisa , Shaveet Eden , Estime Brandon , Patel Avi 
Published: July 2022

AbstractBackgroundOver 50% of U.S. adults do not take their prescriptions as prescribed, which is responsible for 33% to 69% of hospital admissions and 125,000 deaths annually. Given the higher prevalence of prescription drug use among older adult populations, increasing medication adherence is of particular importance with this age group. Two speculated facilitators of medication adherence are home medication storage location and the use of digital health devices.ObjectiveOur objective was to use survey data to investigate the associations between medication storage location and medication adherence among adults 40 years and older. Additionally, we aimed to report preliminary findings about the associations between use of digital health devices and medication adherence in this same population.MethodsWe conducted primary analysis of data sampled from a home medication management survey deployed in November 2021 (n=580). Exploratory analyses were conducted by way of chi2tests and creation of bivariate logistic regression models.ResultsThe most heavily used storage locations were nightstand drawers (27%), kitchen cabinets (25%), and atop bedroom nightstands (23%). Certain medication storage locations were associated with greater medication adherence. Several storage locations were significantly associated with decreased odds of having ever forgotten to take a medication, including kitchen drawers, in refrigerators, atop bedroom nightstands, in nightstand drawers, and backpacks, purses, or bags. Two home medication storage locations were significantly associated with increased odds of having ever forgotten to intake a medication: kitchen cabinets and bathroom vanities. Further, most (94%) survey respondents indicated they would be receptive to guidance about where to store their medications.ConclusionsGiven that all patients need to select a location to store their prescription medication in their homes and that some storage locations are associated with adherence, we believe that an intervention to optimize storage selection may lead to increased adherence. The impact of digital health device usage paired with optimized home medication storage location on medication adherence in older adult populations remains unknown but is worthy of further investigation. Further, we plan to investigate how new device designs can incorporate specific storage locations and contextual cues related to location to promote adherence more effectively.
A dolabralexin-deficient mutant provides insight into specialized diterpenoid metabolism in maize (Zea mays)
Authors: Murphy Katherine M. , Dowd Tyler , Khalil Ahmed , Char Si Nian , Yang Bing , Endelman Benjamin J. , Shih Patrick M. , Topp Christopher , Schmelz Eric A. , Zerbe Philipp 
Published: July 2022

AbstractTwo major groups of maize (Zea mays) specialized metabolites, termed kauralexins and dolabralexins, serve as known or predicted diterpenoid defenses against pathogens, herbivores, and other environmental stressors. To consider physiological roles of the recently discovered dolabralexin pathway, we examined dolabralexin structural diversity, tissue-specificity, and stress-elicited production in a defined biosynthetic pathway mutant. Metabolomics analyses support a larger number of dolabralexin pathway products than previously known. We identified dolabradienol as a previously undetected pathway metabolite and characterized its enzymatic production. Transcript and metabolite profiling showed that dolabralexin biosynthesis and accumulation predominantly occurs in primary roots and shows quantitative variation across genetically diverse inbred lines. Generation and analysis of CRISPR-Cas9-derived loss-of- function Kaurene Synthase-Like 4 (Zmksl4) mutants demonstrated dolabralexin production deficiency, thus supporting ZmKSL4 as the diterpene synthase responsible for the conversion of geranylgeranyl pyrophosphate precursors into dolabradiene and downstream pathway products.Zmksl4mutants further display altered root-to-shoot ratios and root architecture in response to water deficit, consistent with an interactive role of maize dolabralexins in plant vigor during abiotic stress.
Evaluating instruments for assessing healthspan: a multi-center cross-sectional study on health-related quality of life (HRQL) and frailty in the companion dog
Authors: Chen Frances L. , Ullal Tarini V. , Graves Jessica L. , Ratcliff Ellen R. , Naka Alexander , McKenzie Brennen , Carttar Tennery A. , Super Kaitlyn M. , Austriaco Jessica , Weber Sunny Y. , Vaughn Julie , LaCroix-Fralish Michael L. 
Published: July 2022

AbstractDeveloping valid tools that assess key determinants of canine healthspan such as frailty and health-related quality of life (HRQL) is essential to characterizing and understanding aging in dogs. Additionally, because the companion dog is an excellent translational model for humans, such tools can be applied to evaluate gerotherapeutics and investigate mechanisms underlying longevity in both dogs and humans. In this multi-center, cross sectional study, we investigated the use of a clinical questionnaire (Canine Frailty Index; CFI; Banzato et al., 2019) to assess frailty and an owner assessment tool (VetMetrica HRQL) to evaluate HRQL in 451 adult companion dogs. Results demonstrated validity of the tools by confirming expectations that frailty and HRQL deteriorate with age. CFI scores were significantly higher (higher frailty) and HRQL scores significantly lower (worse HRQL) in old dogs (≥ 7 years of age) compared to young dogs (≥ 2 and < 6 years of age). Body size (small < 25lbs or large > 50lbs) was not associated with CFI or total HRQL score. However, older, larger dogs showed faster age-related decline in HRQL scores specific to owner-reported activity and comfort. Findings suggest that the clinician-assessed CFI and owner-reported VetMetrica HRQL are useful tools to evaluate two determinants of healthspan in dogs: the accumulation of frailty and the progressive decline in quality of life. Establishing validated tools that operationalize the assessment of canine healthspan is critical for the advancement of geroscience and the development of gerotherapeutics that benefit both human and veterinary medicine.Graphical abstractGraphical summary of the design, results, and conclusions of the study.
GPC3-Unc5D complex structure and role in cell migration
Authors: Akkermans O , Delloye-Bourgeois C , Peregrina C , Carrasquero-Ordaz M , Kokolaki M , Berbeira-Santana M , Chavent M , Reynaud F , Raj Ritu , Agirre J , Aksu M , White E , Lowe E , Ben Amar D , Zaballa S , Huo J , McCubbin P.T.N. , Comoletti D , Owens R , Robinson C.V. , Castellani V , del Toro D , Seiradake E 
Published: July 2022

SummaryNeural migration is a critical step during brain development that requires the interactions of cell-surface guidance receptors. Cancer cells often hijack these mechanisms to disseminate. Here we reveal crystal structures of Uncoordinated-5 receptor D (Unc5D) in complex with morphogen receptor glypican-3 (GPC3), forming an octameric glycoprotein complex. In the complex, four Unc5D molecules pack into an antiparallel bundle, flanked by four GPC3 molecules. Central glycan-glycan interactions are formed by N-linked glycans emanating from GPC3 (N241 in human) and C-mannosylated tryptophans of the Unc5D thrombospondin-like domains. MD simulations, mass-spectrometry and structure-based mutants validate the crystallographic data. Anti-GPC3 nanobodies enhance or weaken Unc5-GPC3 binding. Using these tools in vivo, we show that Unc5/GPC3 guide migrating pyramidal neurons in the mouse cortex, and cancer cells in an embryonic xenograft neuroblastoma model. The results demonstrate a conserved structural mechanism of cell-guidance, with the potential for wide- ranging biomedical implications in development and cancer biology.
Intrinsic and Rho-dependent termination cooperate for efficient transcription termination at 3’ untranslated regions
Authors: Ahmad Ezaz , Mahapatra Varsha , V M Vanishree , Nagaraja Valakunja 
Published: July 2022

AbstractThe intrinsic, and the Rho-dependent mechanisms of transcription termination are conserved in bacteria. Generally, the two mechanisms have been illustrated as two independent pathways occurring in the 3’ ends of different genes with contrasting requirements to halt RNA synthesis. However, a majority of the intrinsic terminators terminate transcription inefficiently leading to transcriptional read-through. The unwanted transcription in the downstream region beyond the terminator would have undesired consequences. To prevent such transcriptional read-through, bacteria must have evolved ways to terminate transcription more efficiently at or near the termination sites. We describe the participation of both the mechanisms, where intrinsic terminator and Rho factor contribute to prevent transcriptional read-through. Contribution from both the termination processes is demonstrated at the downstream regions of the genes both in vitro and in vivo in mycobacteria. Distinct patterns of cooperation between the two modes of termination were observed at the 3’ untranslated regions of the genes to ensure efficient termination. We demonstrate similar mode of operation between the two termination processes in Escherichia coli suggesting a likely prevalence of this cooperation across bacteria. The reporter system developed to assess the Rho – intrinsic termination collaboration in vivo for mycobacteria and E. coli can readily be applied to other bacteria.
Omnicrobe, an open-access database of microbial habitats and phenotypes using a comprehensive text mining and data fusion approach
Authors: Dérozier Sandra , Bossy Robert , Deléger Louise , Ba Mouhamadou , Chaix Estelle , Harlé Olivier , Loux Valentin , Falentin Hélène , Nédellec Claire 
Published: July 2022

AbstractThe dramatic increase in the amount of microbe descriptions in databases, reports and papers presents a two-fold challenge for accessing the information: integration of heterogeneous data in a standard ontology-based representation and normalization of the textual descriptions by semantic analysis. Recent text mining methods offer powerful ways to extract textual information and generate ontology-based representation.This paper describes the design of the Omnicrobe application that gathers comprehensive information on habitats, phenotypes and usages of microbes from scientific sources of high interest to the microbiology community. The Omnicrobe database contains around 1 million descriptions of microbe properties that are created by analyzing and combining six information sources of various kinds, i.e. biological resource catalogues, sequence database and scientific literature. The microbe properties are indexed by the Ontobiotope ontology and their taxa are indexed by an extended version of the taxonomy maintained by the National Center for Biotechnology Information.The Omnicrobe application covers all domains of microbiology. It provides an easy-to-use support in the resolution of scientific questions related to the habitats, phenotypes and uses of microbes through simple and complex ontology-based queries. We illustrate the potential of Omnicrobe with a use case from the food innovation domain.
Multi-Domain Characterization and Rapid Detection Technology for Cocaine and Methamphetamine Acute Abuse based on EEG
Authors: Xia Jie , Wu Jintao , Pan Jiadong , Zhang Fan , Jin Hao , Dong Shurong , Wang Yueming , Luo Jikui , Si Ke 
Published: July 2022

AbstractMulti-domain characterization and a new fast detection method for acute illegal psychostimulants abuse detection based on waking-state EEG of mice is proposed in this paper. To get corftical electroencephalogram (EEG), three groups of mice were injected with cocaine (Coca), methamphetamine (Meth), and saline (Sali) respectively following the experimental paradigm of drug abuse. The EEG features were extracted out by multi-domain views, including time, frequency, complexity, dynamics, and independent domains to obtain acute drug abuse effects on the brain. New strategy combing time domain with frequency domain is developed as multi-domain input and by means of dimension transformation approach preserving temporal information, the performance of drug abuse detection is greatly improved with deep learning models of ResNet50. Results show that comparing to support vector machine (SVM), k nearest neighbor (kNN), random forest (RF), and long short-term memory fully convolutional networks (LSTM-FCN), ResNet50 based on our proposed multi-domain features has best F1-score of 95.10%. This promising method provides a low-cost, fast, and widely assisted technology for psychostimulants abuse identification.
Exploring Stop Signal Reaction Time Over Two Sessions of the Anticipatory Response Inhibition Task
Authors: Hall Alison , Jenkinson Ned , MacDonald Hayley J. 
Published: July 2022

ABSTRACTVarious behavioural tasks can measure the motor component of impulse control, response inhibition. Response inhibition encompasses the ability to cancel unwanted actions and is evaluated via stop signal reaction time (SSRT). The current study explored the effect of two sessions on SSRT within the anticipatory response inhibition task (ARIT) and how this compared to the stop signal task (SST). Forty-four participants completed two sessions of the ARIT and SST, 24 hours apart. SSRT and its constituent measures (Go trial reaction time, stop signal delay) were calculated. SSRT reflecting non-selective inhibition was consistent between sessions in both tasks (both p > .293). Reaction time and stop signal delay also remained stable across sessions in the ARIT (all p > .063), whereas in the SST, both reaction time (p = .013) and stop signal delay (p = .009) increased. Across the two sessions, SSRT reflecting partial inhibition improved (p < .001), which was underpinned by changes to reaction time (p < .001) and stop signal delay (p < .001). The maximal efficiency of non-selective inhibition remained stable across two sessions in the ARIT. Results of the SST confirmed that non-selective inhibition can however be affected by more than inhibitory network integrity when Go trial reaction times are not constrained in task design. Partial response inhibition measures changed across sessions, suggesting the sequential process captured by the SSRT occurred more quickly in session two. These findings highlight the absence/extent of inherent SSRT changes possible during multiple-session study designs e.g., pre/post, or active/sham comparisons.
Temporal profiling of CD4 T-cell activation and differentiation upon SARS-CoV-2 spike protein immunisation
Authors: Almeida-Santos José , Berkachy Rita , Tye Chanidapa Adele , Hassan Jehanne , Kalfaoglu Bahire , Selkirk Murray E. , Ono Masahiro 
Published: July 2022

ABSTRACTCD4 T-cells require T-cell receptor (TCR) signalling for their activation and differentiation. Foxp3+ regulatory T-cells (Treg) are dependent on TCR signals for their differentiation and suppressive function. However, it is not fully known how TCR signalling controls the differentiation of polyclonal CD4 T-cells upon antigen recognition at the single-cell level in vivo. In this study, using Nr4a3-Tocky (Timer-of-cell-kinetics-and-activity), which analyses temporal changes of antigen-reactive T-cells following TCR signalling, we investigated T-cell response to Spike protein fragments (S1a, S1b, S2a, and S2b) upon immunisation. We show that S1a and S2a induced the differentiation of PD1hiCXCR5+ T follicular helper (Tfh) cells, which is related to CD4 T-cell immunogenicity. In contrast, S1b induced CD25hiGITRhiPD-1int Treg, which intermittently received TCR signalling. Using Foxp3-Tocky, which analyses Foxp3 transcriptional dynamics, the S1b-reactive Treg sustained Foxp3 transcription over time, which is a hallmark of activated Treg. Foxp3 fate-mapping showed that the S1b-reactive Treg were derived not from pre-existing thymic Treg, suggesting Foxp3 induction in non-Treg cells. Thus, the current study reveals temporally dynamic differentiation of CD4 T-cells and Treg upon immunisation in the polyclonal TCR repertoire.
Investigation of the genetic architecture of cam morphology, and its relationship with hip osteoarthritis, using alpha angle as a proxy measure
Authors: Faber Benjamin G. , Frysz Monika , Hartley April E. , Ebsim Raja , Boer Cindy G. , Saunders Fiona R. , Gregory Jennifer S. , Aspden Richard M , Harvey Nicholas C. , Southam Lorraine , Giles William , Maitre Christine Le , Wilkinson J. Mark , van Meurs Joyce B.J. , Zeggini Eleftheria , Cootes Timothy , Lindner Claudia , Kemp John P. , Smith George Davey , Tobias Jonathan H. 
Published: July 2022

ObjectivesTo examine the genetic architecture of cam morphology, using alpha angle (AA) as a proxy measure, we conducted an AA genome wide association study (GWAS), followed by Mendelian randomisation (MR) to evaluate its causal relationship with hip osteoarthritis (HOA).MethodsObservational analyses examined associations between AA derived from hip DXA scans in UK Biobank (UKB), and radiographic HOA (rHOA) and subsequent total hip replacement (THR). Afterwards, an AA GWAS meta-analysis was performed (n=44,214), using AA previously derived in the Rotterdam Study (RS). Linkage disequilibrium score regression assessed the genetic correlation between AA and HOA. Genetic associations with P<5×10−8instrumented AA for two-sample MR.ResultsDXA-derived AA showed expected associations between AA and rHOA (OR 1.63 [95% CI 1.58-1.67]) and THR (HR 1.45 [1.33-1.59]) in UKB. The heritability of AA was 10% and AA had a moderate genetic correlation with HOA (rg=0.26 [0.10-0.43]). Eight independent genetic signals were associated with AA. Two-sample MR provided weak evidence of causal effects of AA on HOA risk (inverse variance weighted (IVW): OR=1.84 [1.14-2.96], P 0.01). In contrast, genetic predisposition for HOA had stronger evidence of a causal effect on increased AA (IVW: β=0.09 [0.04-0.13], P 4.58 × 10−05).ConclusionsExpected observational associations between AA and related clinical outcomes provided face-validity for the DXA-derived AA measures. Evidence of bidirectional associations between AA and HOA, particularly in the reverse direction, suggest that hip shape remodelling secondary to a genetic predisposition to HOA contribute to the well-established relationship between HOA and cam morphology in older adults.
The longitudinal relation between executive functioning and multilayer network topology in glioma patients
Authors: van Lingen Marike R. , Breedt Lucas C. , Geurts Jeroen J.G. , Hillebrand Arjan , Klein Martin , Kouwenhoven Mathilde C.M. , Kulik Shanna D. , Reijneveld Jaap C. , Stam Cornelis J. , De Witt Hamer Philip C. , Zimmermann Mona L.M. , Santos Fernando A.N. , Douw Linda 
Published: July 2022

AbstractMany patients with glioma, primary brain tumors, suffer from poorly understood executive functioning deficits before and/or after tumor resection. We aimed to test whether frontoparietal network centrality of multilayer networks, which allow for integration across multiple frequencies, relates to and predicts executive functioning in glioma patients before and after tumor resection. Patients with glioma (n = 37) underwent neuropsychological tests assessing word fluency, inhibition, and set shifting, and resting-state magnetoencephalography before tumor resection (T1) and one year after resection (T2). We constructed binary multilayer networks comprising six layers, with each layer representing frequency-specific functional connectivity (phase lag index) between source-localized time series of 78 cortical regions. Average frontoparietal network multilayer eigenvector centrality, a measure for network integration, was calculated at both time points. Regression analyses were used to investigate its associations with executive functioning.At T1, lower multilayer integration (p = 0.017) and having epilepsy (p = 0.006) associated with poorer set shifting (adj. R2 = 0.269). Decreasing multilayer integration (p = 0.022) and not undergoing chemotherapy at T2 (p = 0.004) related to deteriorating set shifting (adj. R2 = 0.283). No significant associations were found for word fluency or inhibition, nor did T1 multilayer integration predict changes in executive functioning. As expected, our results establish multilayer integration of the frontoparietal network as a cross-sectional and longitudinal correlate of executive functioning in glioma patients. However, multilayer integration did not significantly predict postoperative changes in executive functioning, limiting its direct clinical relevance.
Gene expression profiles of chemosensory genes of termite soldier and worker antennae
Authors: Suzuki Ryohei H. , Hanada Takumi , Hayashi Yoshinobu , Shigenobu Shuji , Maekawa Kiyoto , Hojo Masaru K. 
Published: July 2022

ABSTRACTTermite caste differentiation and social behavior are appropriately regulated by the chemical signals among individuals in each colony. Signal transduction is well known to be triggered by the reception of odorant molecules by some binding proteins in the antennae, after which, a signal is transmitted to chemosensory receptors. However, there is insufficient information on the role of chemosensory genes involved in signal transduction in termites. Here, we identified the genes involved in chemosensory reception in the rhinotermitid termite Reticulitermes speratus, and performed a genome-wide comparative transcriptome analysis of worker and soldier antennae. First, we identified 31 odorant-binding proteins (OBPs), and three chemosensory protein A (CheA) from the available genome sequence data. Thereafter, we performed RNA sequencing to compare the expression levels of OBPs, CheAs, and previously identified chemosensory receptor genes between workers and soldiers antennae. Of note, there were no receptor genes with significant differences in expression between castes. However, the expression levels of three non-receptor genes (OBP, CheA, and Sensory neuron membrane protein) were significantly different between castes. Quantiative polymerase chain reaction (qPCR) analysis using antennae and other head parts confirmed that these genes were highly expressed in soldier antennae. Finally, independent qPCR analysis showed that the expression patterns of these genes were altered in soldiers from different social contexts. The present results suggest that some non-receptor protein genes are involved in the social behaviors of termites.
Perception of innovation in Spain
Authors: Gijón Covadonga , Albarrán Lozano Irene , Molina José M. 
Published: July 2022

Purpose
The purpose of this paper is to evaluate the innovation perception, in its different aspects, of the Spanish people. To this end, several ordered logit models have been developed to determine the extent to which socio-demographic characteristics and other aspects of innovation affect the perception of innovation.

Design/methodology/approach
This paper analyses the perception of individuals about innovation in Spain and the related factors. It uses 2015 and 2018 data on individuals from Spanish surveys. The data include various measures of innovation perception, age, gender, educational level and other socio-economic variables.

Findings
The results indicate that people have a better perception of innovation if they are being trained in innovation or have good information and communication technologies skills. Among the main results, there is a gender gap in the perception of innovation, as well as differences in terms of digital skills.

Originality/value
To the best of the authors’ knowledge, it is the first time of this kind of analysis in this country. The results are linked to the literature, but should nevertheless be taken into account, which is why some policy recommendations are presented below.