Antioxidant Activity of Brown Seaweed (Padina pavonica (L.) Extracts From the Algerian Mediterranean Coast
Authors: Khadidja KERZABI-KANOUN  , Nabila BELYAGOUBI-BENHAMMOU  , Larbi BELYAGOUBI  , Meriem BENMAHDJOUB  , Ghania AISSAOUI  , Wassila BENGHEDDA  , Fawzia ATIK BEKKARA  
Published: July 2022

The crude methanolic extract, ethyl acetate and butanolic fractions, tannins, saponins and polysaccharides of the marine brown alga, Padina pavonica, growing on the Algerian Mediterranean Coast were measured for antioxidant activity, using total antioxidant capacity (TAC), reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Total phenolic, flavonoid and proanthocyanidin contents were determined. The total phenolic, proanthocyanidin and flavonoid contents of methanolic extract were about of 2.007 ± 0.104 mg GAE/g DM, 4.611 ± 0.346 mg CE/g DM and 1.132 ± 0.091 mg CE/g DM, respectively. The total antioxidant capacity was higher (4.365 ± 0.452 mg AAE/g DM) in crude methanolic extract. For the other two tests, the tannins and ethyl acetate fraction presented high activities compared to the other extracts with EC50 about 1.430 ± 0.000 and 1.615 ± 0.009 mg/mL), respectively for the reducing power and 5.718 ± 1.111 and 5.848 ± 0.186 mg/mL for the DPPH radical scavenging. The seaweed extracts displayed moderate antioxidant activity compared to ascorbic acid.
REFERENCES:
Aidi Wannes, W., Mhamdi, B., Sriti, J., Ben Jemia, M., Ouchikh, O., Hamdaoui, G., Kchouk,M.E., & Marzouk, B. (2010). Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf stem and flower. Food andChemistry Toxicology, 48 (5), 1362–1370.Applebaum, S.W., Marco, S., & Birk, Y. (1969). Saponins as possible factors of resistance oflegume seeds to the attack of insects. Journal of Agricultural and Food Chemistry, 17(3), 618–622.Bekkara, F., Jay, M., Viricel, M.R., & Rome, S. (1998). Distribution of phenolic compoundswithin seed and seedlings of tow Vicia faba cvs differing in their seed tannin content,and study of to their seed and root phenolic exudation. Plant and Soil, 203 (1), 27–36.Ben Said, R., El Abed, A., & Romdhane, M. S. (2002). Etude d’une Population de l’alguebrune Padina pavonica (L) Lamouroux A Cap Zebib (Nord De La Tunisie). Bulletin del'Institut National Scientifique et Technique d'Oceanographie et de Peche deSalammbo, 29, 95–103.Chbani, A., Mawlawi, H., & Eltahiri, S. (2011). Antibacterial activity of brown seaweedextracts of Padina pavonica from the Mediterranean coast of Lebanon. Phytothérapie,9, 283–286.Chbani, A., Mawlawi, H., & Zaouk, L. (2013). Evaluation of brown seaweed (Padinapavonica) as biostimulant of plant growth and development. African Journal ofAgricultural Research, 8 (13), 1155–1165.Demirel, Z., Yildirim, Z.D., Tuney, I., Kesici, K., & Sukatar, A. (2012). Biochemical analysisof some brown seaweeds from Aegean Sea. Botanica Serbica, 36, 91-95.Fischer, W., Scheider, M., & Bauchot, M.L. (1987). Fiches FAO d'identification des espècespour les besoins de la pêche. Méditerranée et Mer noire; Zone de pêche 37. [Speciesidentification sheets FAO for fishery purposes. Mediterranean and black sea; fishing area37].Vol.1. Food and Agriculture Organisation of the United Nations, Rome. p. 758.Indu, H., & Seenivasan, R. (2013). In vitro antioxidant activity of selected seaweeds fromsoutheast coast of India, International Journal of Pharmacy and PharmaceuticalSciences, 5(2), 474–484.Ismail-Ben Ali, A., Ktari, L., Boudabbous, A., & El Bour, M. (2010). Seasonal variation ofantibacterial activity of the brown alga Padina pavonica (L) thivy collected fromNorthern coast of Tunisia. Bulletin de l'Institut National Scientifique et Techniqued'Oceanographie et de Peche de Salammbo, 37, 111–116.Julkunen-Titto, R. (1985). Phenolic constituents in the leaves of northern willows: methodsfor the analysis of certain phenolics. Journal of Agricultural and Food Chemistry, 33(2), 213–217.Kamenarska, Z., Gasic, M. J., Zlatovi, M., Razovic, A., Sladic, D., Kljajic, Z., Stefanov, K.,Seizova, K., Najdenski, H., Kujumgieva, A., Tsvetkova, I., & Popo, S. (2002).Chemical composition of the brown Alga Padina pavonia (L.) Gaill. from the AdriaticSea. Botanica Marina, 45, 339–345.Kokabi, M., Yousefzadi, M., Ali ahmadi, A., Feghhi, M.A., & Keshavarz, M. (2013).Antioxidant Activity of Extracts of Selected Algae from the Persian Gulf, Iran. Journalof the Persian Gulf (Marine Science), 4(6), 45–50.Ksouri, J., Elferjani, H., & Mensi, F. (2008). Estimation du stock naturel de l’algue brunePadina pavonica (L.) Thivy en Tunisie septentrionale (Cap Zebib). Bulletin de l'InstitutNational Scientifique et Technique d'Oceanographie et de Peche de Salammbo, 35, 57–60.Ktari, L., & Guyot, M. (1999). A cytotoxic oxysterol from the marine alga Padina pavonica(L.) Thivy. Journal of Applied Phycology, 11 (6), 511–513.Ktari, L., Longeon, A., & Guyot, M. (2001). Biological activities of marine algae fromTunisian coasts. Journal de Recherche Océanographique, 26, 37–41.
Kumar Chandini, S., Ganesan, P., & Bhaskar, N. (2008). In vitro antioxidant activities ofthree selected brown seaweeds. Food Chemistry, 107, 707–713.Naja, K., Mawlawi, H., & Chbani, A. (2012). Antioxidant and Antifungal activities of PadinaPavonica and SargassumVulgarefrom the Lebanese Mediterranean Coast. Advances inEnvironmental Biology, 6(1), 42–48.Omezzine, F., Haouala, R., El Ayeb, A., & Boughanmi, N. (2009). Allelopathic andantifungal potentialities of Padina pavonica (L.) extract. Journal of Plant Breeding andCrop Science, 1(4), 194–203.Oyaizu, M. (1986). Studies one products of browning reaction prepared from glucose amine.Japanese Journal of Nutrition, 44 (6), 307–315.Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidantcapacity through the formation of a phosphomolybdenum complex: specific applicationto the determination of vitamin E. Analytical Biochemistry, 269 (2), 337–341.Sanchez-Moreno, C., Larrauri, J.A., & Saura-Calixto, F. (1998). A procedure to measure theantiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture,76 (2), 270–276.Sari, A., & Tuzen, M. (2009). Equilibrium, thermodynamic and kinetic studies on aluminumbiosorption from aqueous solution by brown algae (Padina pavonica) biomass.Journal of Hazardous Materials, 171, 973–979Singleton, C.P., & Rossi, J.A. (1965). Colorimetry of Total Phenolics withPhosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enolology andViticulture, 16 (3), 144–158.Teodosio Melo, K.R., Gomes Camara., R.B., Queiroz, M.F., Jacome Vidal, A.A., MachadoLima, K.R., Melo-Silveira, R.F., Almeida-Lima, J., & Oliveira Rocha, H.A. (2013).Evaluation of Sulfated Polysaccharides from the Brown Seaweed Dictyopteris Justii asAntioxidant Agents and as .Inhibitors of the Formation of Calcium Oxalate Crystals.Molecules, 18, 14543–14563Wijesekara, I., Pangestutia, R., & Kima, S.K. (2011). Biological activities and potentialhealth benefits of sulfated polysaccharides derived from marine algae. CarbohydratePolymers, 84, 14–21Zhang, S.Y., Zheng, C.G., Yan, X.Y., & Tian, W.X. (2008). Low concentration of condensedtannins from catechu significantly inhibits fatty acid synthase and growth of MCF-7concealments. Biochemical and Biophysical Research Communications, 371 (4), 654–658.Zhenfei, P., Min, L., Zhexiang, F., Jiulin, W., & Qiqing Z. (2012). Composition andcytotoxicity of a novel polysaccharide from brown alga (Laminaria japonica).Carbohydrate Polymers, 89, 1022– 1026.Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contentin mulberry and to their scanenging effects one superoxide radicals. Food Chemistry,64 (4), 555–559.