bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response of litter decomposition and the soil environment to one-year nitrogen addition in a Schrenk spruce forest in the Tianshan Mountains, China
Authors: Ding Zhaolong , Liu Xu , Gong Lu , Chen Xin , Zhao Jingjing , Chen Wenjing 
Published: January 2022

AbstractHuman activities have increased the input of nitrogen (N) to forest ecosystems and have greatly affected litter decomposition and the soil environment. But differences in forests with different nitrogen deposition backgrounds. To better understand the response of litter decomposition and soil environment of N-limited forest to nitrogen deposition. We established an in situ experiment to simulate the effects of N deposition on soil and litter ecosystem processes in a Picea schrenkiana forest in the Tianshan Mountains, China. This study included four N treatments: control (no N addition), low N addition (LN: 5 kg N ha−1 a−1), medium N addition (MN: 10 kg N ha−1 a−1) and high N addition (HN: 20 kg N ha−1 a−1). Our results showed that N addition had a significant effect on litter decomposition and the soil environment. Litter mass loss in the LN treatment and in the MN treatment was significantly higher than that in the control treatment. In contrast, the amount of litter lost in the HN treatment was significantly lower than the other treatments. N application inhibited the degradation of lignin but promoted the breakdown of cellulose. The carbon (C), N, and phosphorus (P) contents of litter did not differ significantly among the treatments, but LN promoted the release of C and P. Our results also showed that soil pH decreased with increasing nitrogen application rates, while soil enzyme activity showed the opposite trend. In addition, the results of redundancy analysis (RDA) and correlation analyses showed that the soil environment was closely related to litter decomposition. Soil enzymes had a positive effect on litter decomposition rates, and N addition amplified these correlations. Our study confirmed that N application had effects on litter decomposition and the soil environment in a N-limited P. schrenkiana forest. LN had a strong positive effect on litter decomposition and the soil environment, while HN was significantly negative. Therefore, increased N deposition may have a negative effect on material cycling of similar forest ecosystems in the near future.
Automated light-induced synthesis of 89Zr-radiolabeled antibodies for immuno-positron emission tomography
Authors: Klingler Simon , Holland Jason P. 
Published: January 2022

AbstractClinical production of 89Zr-radiolabeled antibodies (89Zr-mAbs) for positron emission tomography imaging relies on the pre-conjugation of desferrioxamine B (DFO) to the purified protein, followed by isolation and characterization of the functionalized intermediate, and then manual radiosynthesis. Although highly successful, this route exposes radiochemists to a potentially large radiation dose and entails several technological and economic hurdles that limit access of 89Zr-mAbs to just a specialist few Nuclear Medicine facilities worldwide. Here, we introduce a fully automated synthesis box that can produce individual doses of 89Zr-mAbs formulated in sterile solution in < 25 min starting from [89Zr(C2O4)4]4– (89Zr-oxalate), our good laboratory practice-compliant photoactivatable desferrioxamine-based chelate (DFO-PEG3-ArN3), and clinical-grade antibodies without the need for pre-purification of protein. The automated steps include neutralization of the 89Zr-oxalate stock, chelate radiolabeling, and light-induced protein conjugation, followed by 89Zr-mAb purification, formulation, and sterile filtration. As proof-of-principle, 89ZrDFO-PEG3-azepin-trastuzumab was synthesized directly from Herceptin in < 25 min with an overall decay-corrected radiochemical yield of 20.1 ± 2.4% (n = 3), a radiochemical purity > 99%, and chemical purity > 99%. The synthesis unit can also produce 89Zr-mAbs via the conventional radiolabeling routes from pre-functionalized DFO-mAbs that are currently used in the clinic. This automated method will improve access to state-of-the-art 89Zr-mAbs at the many Nuclear Medicine and research institutions that require automated devices for radiotracer production.
Recycling of clastics in coastal areas inferred from quantitative analysis of reworked radiocarbon samples
Authors: Tanabe Susumu , Nakanishi Toshimichi , Nakashima Rei 
Published: January 2022

AbstractStudies of the evolution of coastal lowlands since the Last Glacial Maximum (LGM) typically ignore radiocarbon data from sediment samples that have undergone reworking. However, these samples contain information on their sediment sources and the timing of their redeposition. We analyzed 738 radiocarbon dates obtained from shell and plant material in samples of post-LGM coastal sediment from north of Tokyo Bay, Japan. Of these samples, 245 (33%) were reworked. Furthermore, the percentage of reworked samples and their average age offsets increased with the depth of the water environment (terrestrial, 15% and 360 ± 250 years, respectively; intertidal, 26% and 470 ± 620 years; subtidal, 39% and 550 ± 630 years). Taking these radiocarbon samples as a proxy for clastic material, our results imply that channel erosion accounted for relatively little clastic removal in the terrestrial and intertidal environments over short timescales, whereas ~ 40% of clastics were removed by storm winnowing and transported in stepwise fashion to deeper water over longer timescales and ~ 60% in the subtidal environment were transported by floods directly from river mouths. These findings imply that radiocarbon ages from reworked samples can be used to quantify clastic recycling processes and their history in coastal areas.
Identification of long non-coding RNAs and RNA binding proteins in breast cancer subtypes
Authors: Cava Claudia , Armaos Alexandros , Lang Benjamin , Tartaglia Gian G. , Castiglioni Isabella 
Published: January 2022

AbstractBreast cancer is a heterogeneous disease classified into four main subtypes with different clinical outcomes, such as patient survival, prognosis, and relapse. Current genetic tests for the differential diagnosis of BC subtypes showed a poor reproducibility. Therefore, an early and correct diagnosis of molecular subtypes is one of the challenges in the clinic. In the present study, we identified differentially expressed genes, long non-coding RNAs and RNA binding proteins for each BC subtype from a public dataset applying bioinformatics algorithms. In addition, we investigated their interactions and we proposed interacting biomarkers as potential signature specific for each BC subtype. We found a network of only 2 RBPs (RBM20 and PCDH20) and 2 genes (HOXB3 and RASSF7) for luminal A, a network of 21 RBPs and 53 genes for luminal B, a HER2-specific network of 14 RBPs and 30 genes, and a network of 54 RBPs and 302 genes for basal BC. We validated the signature considering their expression levels on an independent dataset evaluating their ability to classify the different molecular subtypes with a machine learning approach. Overall, we achieved good performances of classification with an accuracy >0.80. In addition, we found some interesting novel prognostic biomarkers such as RASSF7 for luminal A, DCTPP1 for luminal B, DHRS11, KLC3, NAGS, and TMEM98 for HER2, and ABHD14A and ADSSL1 for basal. The findings could provide preliminary evidence to identify putative new prognostic biomarkers and therapeutic targets for individual breast cancer subtypes.
Spatial and temporal changes of outdoor thermal stress: influence of urban land cover types
Authors: Rahman Mohammad A. , Franceschi Eleonora , Pattnaik Nayanesh , Moser-Reischl Astrid , Hartmann Christian , Paeth Heiko , Pretzsch Hans , Rötzer Thomas , Pauleit Stephan 
Published: January 2022

AbstractGreen infrastructure (GI) has emerged as a feasible strategy for promoting adaptive capacities of cities to climate change by alleviating urban heat island (UHI) and thus heat stress for humans. However, GI can also intensify the winter cold stress. To understand the extent of UHI within a city as well as the link between outdoor thermal stress both diurnally and seasonally, we carried out an empirical study in Würzburg, Germany from 2018 to 2020. At sub-urban sites, relative humidity and wind speed (WS) was considerably higher and air temperature (AT) lower compared to the inner city sites. Mean AT of inner city sites were higher by 1.3 °C during summer and 5 °C during winter compared to sub-urban sites. The magnitude followed the spatial land use patterns, in particular the amount of buildings. Consequently, out of 97 hot days (AT > 30 °C) in 3 years, 9 days above the extreme threshold of wet bulb globe temperature of 35 °C were recorded at a centre location compared to none at a sub-urban site. Extreme heat stress could be halved with 30–40% cover of greenspaces including grass lawns, green roofs, and green walls with little compromise in increasing winter cold stress.
Dynamic compression of water to conditions in ice giant interiors
Authors: Gleason A. E. , Rittman D. R. , Bolme C. A. , Galtier E. , Lee H. J. , Granados E. , Ali S. , Lazicki A. , Swift D. , Celliers P. , Militzer B. , Stanley S. , Mao W. L. 
Published: January 2022

AbstractRecent discoveries of water-rich Neptune-like exoplanets require a more detailed understanding of the phase diagram of H2O at pressure–temperature conditions relevant to their planetary interiors. The unusual non-dipolar magnetic fields of ice giant planets, produced by convecting liquid ionic water, are influenced by exotic high-pressure states of H2O—yet the structure of ice in this state is challenging to determine experimentally. Here we present X-ray diffraction evidence of a body-centered cubic (BCC) structured H2O ice at 200 GPa and ~ 5000 K, deemed ice XIX, using the X-ray Free Electron Laser of the Linac Coherent Light Source to probe the structure of the oxygen sub-lattice during dynamic compression. Although several cubic or orthorhombic structures have been predicted to be the stable structure at these conditions, we show this BCC ice phase is stable to multi-Mbar pressures and temperatures near the melt boundary. This suggests variable and increased electrical conductivity to greater depths in ice giant planets that may promote the generation of multipolar magnetic fields.
TcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens
Authors: do Amaral Milena , Freitas Ana Camila Oliveira , Santos Ariana Silva , dos Santos Everton Cruz , Ferreira Monaliza Macêdo , da Silva Gesteira Abelmon , Gramacho Karina Peres , Marinho-Prado Jeanne Scardini , Pirovani Carlos Priminho 
Published: January 2022

AbstractProtease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10–7 mol L−1. The thermostability analysis of rTcTI showed that 100% inhibitory activity was retained up to 60 °C and that at 70–80 °C, inhibitory activity remained above 50%. Circular dichroism analysis indicated that the protein is rich in loop structures and β-conformations. Furthermore, in vivo assays against Helicoverpa armigera larvae were also performed with rTcTI in 0.1 mg mL−1 spray solutions on leaf surfaces, which reduced larval growth by 70% compared to the control treatment. Trials with cocoa plants infected with Mp showed a greater accumulation of TcTI in resistant varieties of T. cacao, so this regulation may be associated with different isoforms of TcTI. This inhibitor has biochemical characteristics suitable for biotechnological applications as well as in resistance studies of T. cacao and other crops.
Déjà vu: a reappraisal of the taphonomy of quarry VM4 of the Early Pleistocene site of Venta Micena (Baza Basin, SE Spain)
Authors: Palmqvist Paul , Espigares M. Patrocinio , Pérez-Claros Juan A. , Figueirido Borja , Guerra-Merchán Antonio , Ros-Montoya Sergio , Rodríguez-Gómez Guillermo , García-Aguilar José Manuel , Granados Alejandro , Martínez-Navarro Bienvenido 
Published: January 2022

AbstractVenta Micena, an Early Pleistocene site of the Baza Basin (SE Spain), preserves a rich and diverse assemblage of large mammals. VM3, the main excavation quarry of the site, has been interpreted as a den of the giant hyaenaPachycrocuta brevirostrisin the plain that surrounded the Baza palaeolake. Taphonomic analysis of VM3 has shown that the hyaenas scavenged the prey previously hunted by the hypercarnivores, transported their remains to the communal den, and consumed the skeletal parts according to their marrow contents and mineral density. In a recent paper (Luzón et al. in Sci Rep 11:13977,https://doi.org/10.1038/s41598-021-93261-1, 2021), a small sample of remains unearthed from VM4, an excavation quarry ~ 350 m distant from VM3, is analysed. The authors indicate several differences in the taphonomic features of this assemblage with VM3, and even suggest that a different carnivore could have been the agent involved in the bone accumulation process. Here, we make a comparative analysis of both quarries and analyse more skeletal remains from VM4. Our results indicate that the assemblages are broadly similar in composition, except for slight differences in the frequency of megaherbivores, carnivores and equids according to NISP values (but not to MNI counts), the degree of bone weathering, and the intensity of bone processing by the hyaenas. Given that VM4 and VM3 were not coeval denning areas ofP. brevirostris, these differences suggest that during the years when the skeletal remains were accumulated by the hyaenas at VM3, the rise of the water table of the Baza palaeolake that capped with limestone the bones was delayed compared to VM4, which resulted in their more in-depth consumption by the hyaenas.
Lifespan prolonging mechanisms and insulin upregulation without fat accumulation in long-lived reproductives of a higher termite
Authors: Séité Sarah , Harrison Mark C. , Sillam-Dussès David , Lupoli Roland , Van Dooren Tom J. M. , Robert Alain , Poissonnier Laure-Anne , Lemainque Arnaud , Renault David , Acket Sébastien , Andrieu Muriel , Viscarra José , Sul Hei Sook , de Beer Z. Wilhelm , Bornberg-Bauer Erich , Vasseur-Cognet Mireille 
Published: January 2022

AbstractKings and queens of eusocial termites can live for decades, while queens sustain a nearly maximal fertility. To investigate the molecular mechanisms underlying their long lifespan, we carried out transcriptomics, lipidomics and metabolomics inMacrotermes natalensison sterile short-lived workers, long-lived kings and five stages spanning twenty years of adult queen maturation. Reproductives share gene expression differences from workers in agreement with a reduction of several aging-related processes, involving upregulation of DNA damage repair and mitochondrial functions. Anti-oxidant gene expression is downregulated, while peroxidability of membranes in queens decreases. Against expectations, we observed an upregulated gene expression in fat bodies of reproductives of several components of the IIS pathway, including an insulin-like peptide,Ilp9. This pattern does not lead to deleterious fat storage in physogastric queens, while simple sugars dominate in their hemolymph and large amounts of resources are allocated towards oogenesis. Our findings support the notion that all processes causing aging need to be addressed simultaneously in order to prevent it.
Effective prediction of biosynthetic pathway genes involved in bioactive polyphyllins in Paris polyphylla
Authors: Hua Xin , Song Wei , Wang Kangzong , Yin Xue , Hao Changqi , Duan Baozhong , Xu Zhichao , Su Tongbing , Xue Zheyong 
Published: January 2022

AbstractThe genes in polyphyllins pathway mixed with other steroid biosynthetic genes form an extremely complex biosynthetic network in Paris polyphylla with a giant genome. The lack of genomic data and tissue specificity causes the study of the biosynthetic pathway notably difficult. Here, we report an effective method for the prediction of key genes of polyphyllin biosynthesis. Full-length transcriptome from eight different organs via hybrid sequencing of next generation sequencingand third generation sequencing platforms annotated two 2,3-oxidosqualene cyclases (OSCs), 216 cytochrome P450s (CYPs), and 199 UDP glycosyltransferases (UGTs). Combining metabolic differences, gene-weighted co-expression network analysis, and phylogenetic trees, the candidate ranges of OSC, CYP, and UGT genes were further narrowed down to 2, 15, and 24, respectively. Beside the three previously characterized CYPs, we identified the OSC involved in the synthesis of cycloartenol and the UGT (PpUGT73CR1) at the C-3 position of diosgenin and pennogenin in P. polyphylla. This study provides an idea for the investigation of gene cluster deficiency biosynthesis pathways in medicinal plants.
Characterization of phage resistance and phages capable of intestinal decolonization of carbapenem-resistant Klebsiella pneumoniae in mice
Authors: Fang Qingqing , Feng Yu , McNally Alan , Zong Zhiyong 
Published: January 2022

AbstractCarbapenem-resistant Klebsiella pneumoniae (CRKP) has emerged as a severe global health challenge. We isolate and characterize two previously unidentified lytic phages, P24 and P39, with large burst sizes active against ST11 KL64, a major CRKP lineage. P24 and P39 represent species of the genera Przondovirus (Studiervirinae subfamily) and Webervirus (Drexlerviridae family), respectively. P24 and P39 together restrain CRKP growth to nearly 8 h. Phage-resistant mutants exhibit reduced capsule production and decreased virulence. Modifications in mshA and wcaJ encoding capsule polysaccharide synthesis mediate P24 resistance whilst mutations in epsJ encoding exopolysaccharide synthesis cause P39 resistance. We test P24 alone and together with P39 for decolonizing CRKP using mouse intestinal colonization models. Bacterial load shed decrease significantly in mice treated with P24 and P39. In conclusion, we report the characterization of two previously unidentified lytic phages against CRKP, revealing phage resistance mechanisms and demonstrating the potential of lytic phages for intestinal decolonization.
Exploring nonequilibrium phases of photo-doped Mott insulators with generalized Gibbs ensembles
Authors: Murakami Yuta , Takayoshi Shintaro , Kaneko Tatsuya , Sun Zhiyuan , Golež Denis , Millis Andrew J. , Werner Philipp 
Published: January 2022

AbstractMany experiments show that strong excitations of correlated quantum materials can cause non-thermal phases without equilibrium analogues. Understanding the origin and properties of these nonequilibrium states has been challenging due to the limitations of theoretical methods for nonequilibrium strongly correlated systems. In this work, we introduce a generalized Gibbs ensemble description that enables a systematic analysis of the long-time behavior of photo-doped states in Mott insulators based on equilibrium methods. We demonstrate the power of the method by mapping out the nonequilibrium phase diagram of the one-dimensional extended Hubbard model, which features η-pairing and charge density wave phases in a wide photo-doping range. We furthermore clarify that the peculiar kinematics of photo-doped carriers, and the interaction between them, play an essential role in the formation of these non-thermal phases. Our results establish a new path for the systematic analysis of nonequilibrium strongly correlated systems.
Identification of a regulatory pathway inhibiting adipogenesis via RSPO2
Authors: Dong Hua , Sun Wenfei , Shen Yang , Baláz Miroslav , Balázová Lucia , Ding Lianggong , Löffler Mona , Hamilton Bradford , Klöting Nora , Blüher Matthias , Neubauer Heike , Klein Holger , Wolfrum Christian 
Published: January 2022

AbstractHealthy adipose tissue remodeling depends on the balance between de novo adipogenesis from adipogenic progenitor cells and the hypertrophy of adipocytes. De novo adipogenesis has been shown to promote healthy adipose tissue expansion, which confers protection from obesity-associated insulin resistance. Here, we define the role and trajectory of different adipogenic precursor subpopulations and further delineate the mechanism and cellular trajectory of adipogenesis, using single-cell RNA-sequencing datasets of murine adipogenic precursors. We identify Rspo2 as a functional regulator of adipogenesis, which is secreted by a subset of CD142+ cells to inhibit maturation of early progenitors through the receptor Lgr4. Increased circulating RSPO2 in mice leads to adipose tissue hypertrophy and insulin resistance and increased RSPO2 levels in male obese individuals correlate with impaired glucose homeostasis. Taken together, these findings identify a complex cellular crosstalk that inhibits adipogenesis and impairs adipose tissue homeostasis.
A digital‐controlled gate charge detection circuit for short‐circuit protection and condition monitoring of SiC MOSFET
Authors: Yin Shan , Liu Yinyu , Xin Xiong , Wu Yingzhe , Li Hui 
Published: January 2022

AbstractThe safe and reliable operation of power converter requires a protection circuit with fast response when the power device is subjected to the short‐circuit (SC) fault. In addition, the on‐line condition monitoring circuit is beneficial to provide a suggestion for the replacement of aged device. The silicon carbide (SiC) power devices promise the higher power density in the system performance, but is still limited by the poor SC reliability issue. Thus, it is crucial to improve the robustness of SiC‐based power converter from the circuit design. This work proposes a novel gate charge detection circuit for SiC devices. It achieves a high‐speed SC protection against hard switching fault (HSF) based on the difference of Miller capacitance between normal condition and SC fault. Meanwhile, the condition monitoring is achieved based on the high gate leakage current of aged device. A high‐precision analog amplifier circuit is designed to acquire the gate signal. The normal/fault signal is distinguished and the protection is triggered using a digital‐controlled method to reduce the hardware cost. A 600‐ns SC response time is achieved, and the monitoring circuit can differentiate the aged device from the healthy one.