bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supported SnS2 Nanosheet Arrays on Ni Foam for Supercapacitors
Authors: Sun Haibin , Liang Shuangshuang , Zheng Wenrui , Liu Congcong , Zhang Chao , Ji Zhichao , Liu Shenghong , Xie Wenhe 
Published: April 2022

Layered metal sulfides are regarded as potential candidates for supercapacitive electrode materials due to the unique spatial dimensions for charge transport. Herein, self-supported SnS2 nanosheet arrays on nickel (Ni) foam were successfully fabricated via a facile solvothermal approach. Interestingly, the continuous and high-density SnS2 nanosheet arrays are interconnected to form porous Ni@SnS2 electrode materials, which suppress the self-aggregation of SnS2 and provide outstanding conductivity with 3D-networked Ni framework. The Ni@SnS2 electrode demonstrates a high areal specific capacitance of 1965.56 mF cm−2 at a current density of 1 mA cm−2 and satisfactory cycling stability (78.3% capacity retention after 10,000 cycles). This self-supported porous structure provides a promising way to build advanced electrode material for supercapacitors.
Habitat Characteristics Supporting Bird Species Richness in Mid-Field Woodlots
Authors: Dvořáková Lenka , Kuczyński Lechosław , Rivas-Salvador Javier , Reif Jiří 
Published: April 2022

Farmland biodiversity has undergone drastic declines due to agricultural intensification during the last decades. To prevent further biodiversity loss, the maintenance and restoration of non-productive habitats is essential. Woodlots, small patches of woody vegetation in agricultural landscapes, are one such habitat that are currently subsidized by the European Union’s Common Agricultural Policy (EU’s CAP). For effective implementation, however, it is necessary to assess what habitat characteristics are the most beneficial for biodiversity. Our study performs such an assessment using birds as model organisms. Specifically, we related characteristics of various woodlots to (I) the species richness of all birds, and (II) the species richness of both forest and farmland birds–groups with different ecological requirements. For this purpose, we counted birds (27 farmland and 26 forest species) and measured habitat characteristics (describing vegetation structure, diversity and nativeness) and spatial characteristics (area, shape and isolation) in 82 mid-field woodlots (0.76–1.25 ha, average 0.46 ha) in the Czech Republic (Central Europe). After accounting for the effects of spatial characteristics, overall bird species richness increased with vegetation height and woody plant species richness. In addition, richness showed a non-linear decrease with the cover of an invasive tree, the Black Locust Robinia pseudoaccacia. Interestingly, forest bird species richness was related to the same habitat characteristics as the overall bird species richness. By contrast, farmland bird species richness was positively related to the diversity of woodlot microhabitats, which reflects mainly enrichment by non-forest sites such as grassland or sparse shrubs. Our results indicate that the ecological performance of habitat characteristics (and not only the woodlot area) is important for the restoration of bird diversity in woodlots, and as such should be taken into consideration by the EU’s CAP subsidy system. Moreover, if woodlot management aims to maximize the overall bird diversity—a common practice in biodiversity conservation—our results show that current practices may favor widespread forest bird species, but not the farmland birds that are more threatened in Europe. To manage the woodlot habitat for farmland birds, we suggest that microhabitat heterogeneity should be maximized.
Identification of Potential Diagnoses Based on Immune Infiltration and Autophagy Characteristics in Major Depressive Disorder
Authors: Sun Ye , Li Jinying , Wang Lin , Cong Ting , Zhai Xiuli , Li Liya , Wu Haikuo , Li Shouxin , Xiao Zhaoyang 
Published: April 2022

Background: Major depressive disorder (MDD) is a serious mental illness characterized by mood changes and high suicide rates. However, no studies are available to support a blood test method for MDD diagnosis. The objective of this research was to identify potential peripheral blood biomarkers for MDD and characterize the novel pathophysiology.Methods: We accessed whole blood microarray sequencing data for MDD and control samples from public databases. Biological functions were analysed by GO and KEGG pathway enrichment analyses using the clusterprofile R package. Infiltrated immune cell (IIC) proportions were identified using the CIBERSORT algorithm. Clustering was performed using the ConsensusClusterPlus R package. Protein–protein interactions (PPI) were assessed by constructing a PPI network using STRING and visualized using Cytoscape software. Rats were exposed to chronic unpredictable mild stress (CUMS) for 6 weeks to induce stress behaviour. Stress behaviour was evaluated by open field experiments and forced swimming tests. Flow cytometry was used to analyse the proportion of CD8+ T cells. The expression of the corresponding key genes was detected by qRT–PCR.Results: We divided MDD patients into CD8H and CD8L clusters. The functional enrichment of marker genes in the CD8H cluster indicated that autophagy-related terms and pathways were significantly enriched. Furthermore, we obtained 110 autophagy-related marker genes (ARMGs) in the CD8H cluster through intersection analysis. GO and KEGG analyses further showed that these ARMGs may regulate a variety of autophagy processes and be involved in the onset and advancement of MDD. Finally, 10 key ARMGs were identified through PPI analysis: RAB1A, GNAI3, VAMP7, RAB33B, MYC, LAMP2, RAB11A, HIF1A, KIF5B, and PTEN. In the CUMS model, flow cytometric analysis confirmed the above findings. qRT–PCR revealed significant decreases in the mRNA levels of Gnai3, Rab33b, Lamp2, and Kif5b in the CUMS groups.Conclusion: In this study, MDD was divided into two subtypes. We combined immune infiltrating CD8+ T cells with autophagy-related genes and screened a total of 10 ARMG genes. In particular, RAB1A, GNAI3, RAB33B, LAMP2, and KIF5B were first reported in MDD. These genes may offer new hope for the clinical diagnosis of MDD.
Description of the Molecular and Phenotypic Spectrum of Lesch-Nyhan Disease in Eight Chinese Patients
Authors: Li Lu , Qiao Xiaohui , Liu Fei , Wang Jingjing , Shen Huijun , Fu Haidong , Mao Jian-Hua 
Published: April 2022

Background: Lesch-Nyhan disease (LND) is a rare disorder involving pathogenic variants in the HPRT1 gene encoding the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT) that result in hyperuricemia, intellectual disability, dystonic movement disorder, and compulsive self-mutilation. The purpose of the present study was to characterize the genetic basis of LND and describe its phenotypic heterogeneity by identifying the variation in the HPRT1 gene in a cohort of Chinese LND patients.Results: The median age at diagnosis was 31 mo (interquartile range (IQR): 7–76 mo), and the initial manifestations were mainly head control weakness and motor development delay. The median age of self-mutilation behavior onset was 19 mo (IQR: 17–24 mo), and all patients were required to travel in a wheelchair and fall into the predicament of compulsive self-harm behavior. There were two patients whose blood uric acid levels were normal for their high urinary acid excretion fraction without taking uric acid-lowering drugs. Seven different pathogenic variants of the HPRT1 gene were identified among eight independent pedigrees, including four novel mutations [c.299 (exon 3) T &gt; A; loss (exon: 6) 84 bp; c.277_281delATTGC; c.468_470delGAT]. The pathogenic variant sites were mainly concentrated in exon 3, and truncating mutations (including frameshift mutations and nonsense mutations) were the most common genetic variant types (5/7, 71.4%).Conclusion: The present study described the phenotypic and molecular spectrum of LND in eight Chinese families, including four novel mutations, which expands our understanding of LND.
Impact of Consolidative Unrelated Cord Blood Transplantation on Clinical Outcomes of Patients With Relapsed/Refractory Acute B Lymphoblastic Leukemia Entering Remission Following CD19 Chimeric Antigen Receptor T Cells
Authors: Xu Qianwen , Xue Lei , An Furun , Xu Hui , Wang Li , Geng Liangquan , Zhang Xuhan , Song Kaidi , Yao Wen , Wan Xiang , Tong Juan , Liu Huilan , Liu Xin , Zhu Xiaoyu , Zhai Zhimin , Sun Zimin , Wang Xingbing 
Published: April 2022

BackgroundWhile chimeric antigen receptor (CAR)-T cell therapy is becoming widely used in hematological malignancies with remarkable remission rate, their high recurrence remains an obstacle to overcome. The role of consolidative transplantation following CAR-T cell-mediated remission remains controversial. We conducted a retrospective study to explore whether bridging to unrelated cord blood transplantation (UCBT) could improve the prognosis of patients entering remission after CAR-T therapy with different characteristics through subgroup analyses.MethodsWe reviewed 53 patients with relapsed/refractory (R/R) B-cell acute lymphoblastic leukemia (B-ALL) successfully infused with CD19 CAR-T cells and achieved complete remission (CR). In this study, 25 patients received consolidative UCBT (UCBT group) and 28 patients did not accept any intervention until relapse (non-UCBT group). Subgroup analysis on prognosis was then performed according to gender, age, number of previous relapses, tumor burden, presence of poor prognostic markers, and structure of CAR.ResultsCompared with the non-UCBT group, patients who underwent consolidative UCBT had better median event-free survival (EFS; 12.3 months vs. 6.2 months; P = 0.035) and relapse-free survival (RFS; 22.3 months vs. 7.2 months; P = 0.046), while no significant difference was found in overall survival (OS; 30.8 months vs. 15.3 months; P = 0.118). Subsequent multivariate analysis revealed that bridging to UCBT was a protective factor for RFS (P = 0.048) but had no significant effect on EFS (P = 0.205) or OS (P = 0.541). In the subgroup analysis, UCBT has an added benefit in patients with specific characteristics. Patients who experienced ≥2 relapses or with sustained non-remission (NR) showed better RFS (P = 0.025) after UCBT. Better EFS was seen in patients with poor prognostic markers (P = 0.027). In the subgroup with pre-infusion minimal residual disease (MRD) ≥5% or with extramedullary disease (EMD), UCBT significantly prolonged EFS (P = 0.009), RFS (P = 0.017), and OS (P = 0.026). Patients with occurrence of acute graft-versus-host disease (aGVHD) appeared to have a longer duration of remission (P = 0.007).ConclusionConsolidative UCBT can, to some extent, improve clinical outcomes of patients with R/R B-ALL entering remission following CD19 CAR-T therapy, especially in patients with more recurrences before treatment, patients with poor prognostic markers, and patients with a higher tumor burden. The occurrence of aGVHD after UCBT was associated with better RFS.
Multi-Tube Helmholtz Resonator Based Triboelectric Nanogenerator for Broadband Acoustic Energy Harvesting
Authors: Zhang Qiqi , Xi Ziyue , Wang Yawei , Liu Ling , Yu Hongyong , Wang Hao , Xu Minyi 
Published: April 2022

Acoustic energy, especially broadband low-frequency sound energy is part of the environmental mechanical energy acquisition cannot be ignored. Herein, a multi-tube parallel Helmholtz resonator-based triboelectric nanogenerator (MH-TENG) is investigated to reap sound energy in low-frequency noise environments. The designed MH-TENG consists of a modified Helmholtz resonator and a thin-film TENG transducer. The core materials of the TENG transducer are aluminum, FEP film, and carbon. To further clarify the influence of the modified Helmholtz resonator on the conversion performance of MH-TENG, the acoustic characteristics of the improved resonators are systematically studied. A series of experiments show that the multi-tube parallel Helmholtz resonator structure has a better sound wave collection effect. Meanwhile, the flexible film TENG can reduce the optimal output frequency of the device. The power generation performance and the bandwidth of the MH-TENG are significantly improved by adopting a multi-tube Helmholtz resonator. Within the frequency bandwidth range of 230 Hz, MH-TENG can effectively improve the efficiency of acoustic energy harvesting. 110 LEDs and an electronic thermometer can be powered by the sound-driven MH-TENG. In addition, the MH-TENG has a good capacitor charging performance, which is conducive to its application in ambient sound energy harvesting.
CMOS MEMS Design and Fabrication Platform
Authors: Tseng Sheng-Hsiang 
Published: April 2022

This article mainly describes the technology related to the CMOS MEMS process platform provided by the Taiwan Semiconductor Research Institute (TSRI), including the process flow, design verification, back-end dicing, and packaging of the CMOS MEMS integrated sensing single chip. The front-end CMOS processes can be a standard 0.35 or 0.18 µm CMOS process, or even a BCD high-voltage process. Some academic designs also utilize this platform for in-house post-CMOS process. Finally, the article also explains in detail the CMOS MEMS design flowchart and implementation method provided by TSRI.
Blue Light Induces RPE Cell Necroptosis, Which Can Be Inhibited by Minocycline
Authors: Song Weilin , Zhu Ruilin , Gao Wenna , Xing Chen , Yang Liu 
Published: April 2022

Purpose:Damage to and death of the retinal pigment epithelium (RPE) are closely related to retinal degeneration. Blue light is a high-energy light that causes RPE damage and triggers inflammatory responses. This study investigates whether blue light induces RPE necroptosis, explores pharmacologic therapy and specific mechanisms, and provides hints for research on retinal degeneration.MethodsThe human RPE cell line ARPE-19 was cultured and subjected to blue light insult in vitro. Annexin V/PI was used to evaluate RPE survival. Minocycline was applied to inhibit the death of RPE. Proteomic measurement was used to analyze protein expression. Inhibitors of necroptosis and apoptosis were applied to assess the death mode. Immunofluorescence of protein markers was detected to analyze the mechanism of cell death. Subcellular structural changes were detected by transmission electron microscopy. Reactive oxygen species (ROS) was tested by DCFH-DA. Mitochondrial membrane potential (Δψm) was detected by JC-1. BALB/c mice received bule light exposure, and RPE flatmounts were stained for verification in vivo.ResultsBlue light illumination induced RPE death, and minocycline significantly diminished RPE death. Proteomic measurement showed that minocycline effectively mitigated protein hydrolysis and protein synthesis disorders. Necroptosis inhibitors (Nec-1s, GSK-872) increased the survival of RPE cells, but apoptosis inhibitors (Z-VAD-FMK) did not. After blue light illumination, high-mobility group box-1 (HMGB1) was released from the nucleus, receptor-interacting protein kinase 3 (RIPK3) aggregated, and mixed-lineage kinase domain-like protein (MLKL) increased in the RPE. The application of minocycline alleviated the above phenomena. After blue light illumination, RPE cells exhibited necrotic characteristics accompanied by destruction of cell membranes and vacuole formation, but nuclear membranes remained intact. Minocycline improved the morphology of RPE. Blue light increased ROS and decreased Δψm of RPE, minocycline did not reduce ROS but kept Δψm stable. In vivo, HMGB1 release and RIPK3 aggregation appeared in the RPE of BALB/c mice after blue light illumination, and minocycline alleviated this effect.ConclusionsBlue light exposure causes RPE necroptosis. Minocycline reduces the death of RPE by keeping Δψm stable, inhibiting necroptosis, and preventing HMGB1 release. These results provide new ideas for the pathogenesis and treatment of retinal degeneration.
Identification of Prognostic Factors in Patients With Streptococcus Bloodstream Infection
Authors: Duan Xiaoguang , Zhang Ruifang , Zhang Xiaojuan , Ding Xianfei , Sun Tongwen 
Published: April 2022

AimThe purpose of this study was to explore prognostic factors of bloodstream infections (BSIs), a common severe infection and a major cause of mortality worldwide, so as to construct a prognosis model of patients with BSI.Materials and MethodsClinical and biochemical test data were obtained retrospectively from the medical records of 562 patients with BSI who had been treated at a single center; the end point was 60 days of all-cause death. The chi-square test was used to compare the mortality of patients grouped by the types of antibiotic treatment. The logistic regression analysis was adopted to identify prognostic factors; the Kaplan–Meier survival curve and log-rank test were conducted to compare the survival rate of patients with different prognostic factors; the receiver operating characteristic (ROC) curve was used to estimate the predictive value of different prognostic factors.ResultsOf the 562 patients, 455 survived (80.96%), and 107 died (19.04%). The mortality rate of patients treated with a combination of antibiotics (25.40%) was higher than that treated with a single antibiotic (15.82%). Univariate analysis identified 19 prognostic factors for patients with BSI, including gender, age, diabetes, malignant tumor (non-blood system), total hospitalization time, alanine aminotransferase, aspartate aminotransferase, total protein, albumin, total bilirubin, direct bilirubin, creatinine, ratio of granulocytes, fibrinogen, D-dimer, platelet, C-reactive protein, shock, and respiratory failure (P &lt; 0.05). Multivariate analysis indicated that albumin (odds ratio [OR] = 0.94, 95% confidence interval [CI]: 0.89–0.99), fibrinogen (OR = 0.61, 95%CI: 0.46–0.82), shock (OR = 16.61, 95%CI: 7.00–39.41), and respiratory failure (OR = 47.53, 95%CI: 19.93–133.64) were independent factors. The combination of four indicators demonstrated a favorable predictive value for the 60-day outcome of patients with BSI, with an area under the ROC of 0.96 (95%CI: 0.94–0.99), sensitivity of 90.65%, specificity of 94.95%, and accuracy of 94.13%.ConclusionsShock, respiratory failure, albumin, and fibrinogen are potential independent prognostic factors for 60-day mortality.
Evaluation of Performance of Detection of Immunoglobulin G and Immunoglobulin M Antibody Against Spike Protein of SARS-CoV-2 by a Rapid Kit in a Real-Life Hospital Setting
Authors: Irungbam Monica , Chitkara Anubhuti , Singh Vijay Kumar , Sonkar Subash Chandra , Dubey Abhisek , Bansal Aastha , Shrivastava Ritika , Goswami Binita , Manchanda Vikas , Saxena Sonal , Saxena Ritu , Garg Sandeep , Husain Farah , Talukdar Tanmay , Kumar Dinesh , Koner Bidhan Chandra 
Published: April 2022

BackgroundAntibody testing is often used for serosurveillance of coronavirus disease 2019 (COVID-19). Enzyme-linked immunosorbent assay and chemiluminescence-based antibody tests are quite sensitive and specific for such serological testing. Rapid antibody tests against different antigens are developed and effectively used for this purpose. However, their diagnostic efficiency, especially in real-life hospital setting, needs to be evaluated. Thus, the present study was conducted in a dedicated COVID-19 hospital in New Delhi, India, to evaluate the diagnostic efficacy of a rapid antibody kit against the receptor-binding domain (RBD) of the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).MethodsSixty COVID-19 confirmed cases by reverse transcriptase–polymerase chain reaction (RT-PCR) were recruited and categorized as early, intermediate, and late cases based on the days passed after their first RT-PCR–positive test report, with 20 subjects in each category. Twenty samples from pre-COVID era and 20 RT-PCR–negative collected during the study period were taken as controls. immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against the RBD of the spike (S) protein of SARS-CoV-2 virus were detected by rapid antibody test and compared with the total antibody against the nucleocapsid (N) antigen of SARS-CoV-2 by electrochemiluminescence-based immunoassay (ECLIA).ResultsThe detection of IgM against the RBD of the spike protein by rapid kit was less sensitive and less specific for the diagnosis of SARS-CoV-2 infection. However, diagnostic efficacy of IgG by rapid kit was highly sensitive and specific when compared with the total antibody against N antigen measured by ECLIA.ConclusionIt can be concluded that detection of IgM against the RBD of S protein by rapid kit is less effective, but IgG detection can be used as an effective diagnostic tool for SARS-CoV-2 infection in real-life hospital setting.
Effects of the Lipid Metabolites and the Gut Microbiota in ApoE−/− Mice on Atherosclerosis Co-Depression From the Microbiota-Gut-Brain Axis
Authors: Hu Ke , Liao Xing-Xing , Wu Xiao-Yun , Wang Rui , Hu Zi-Wei , Liu Si-Yuan , He Wen-Fen , Zhou Jun-Jie 
Published: April 2022

Background: The diagnosis, treatment, and prevention of atherosclerosis co-depression are poor, so it is urgent to explore new targets. Based on the “microbiota-gut-brain axis,” this study aimed to investigate the changes of lipid metabolites in the prefrontal cortex and hippocampus regions and the characteristics of the gut microbiota in ApoE−/− mice with atherosclerosis co-depression.Methods: ApoE−/− mice (hyperlipid feeding combined with binding, HFB group, n = 14, male) fed a high-fat diet for 16 weeks with binding stimulation were used as an animal model for atherosclerosis co-depression. The depression degree of mice was evaluated by body weight, sucrose preference test, open field test, and tail suspension test. Oil-red O staining, HE staining, and biochemical parameters were used to evaluate the damage degree of atherosclerosis in mice. LC-MS/MS technique for non-targeted lipidomics analysis was used to analyze the differential lipid metabolites in the prefrontal cortex and hippocampus regions of mice. 16S rDNA amplification sequencing was used to screen the differential gut microbial, and association analysis was performed with the differential lipid metabolites.Results: Compared with the normal control group (NC group), the HFB group showed depression-like behaviors and atherosclerosis-related pathological indicators. The differential lipid metabolites in the prefrontal cortex and hippocampus regions were mainly LPC, LPE, LPS, PC, PE, PS, PI, and GD1a, and were mainly enriched in the glycerophospholipid metabolism pathway and the retrograde endocannabinoid signaling pathway. At the same time, there were significant differences in the structure of the gut microbial community between the two groups. The abundance of Deferribacteres and Proteobacteria in the HFB group increased, while the abundance of Verrucomicrobia and Actinobacteria decreased at the phylum level; the abundance of Desulfovibrio, Clostridium_IV, Helicobacter and Pseudoflavonifractor increased, while the abundance of Akkermansia decreased at the genus level.Conclusion: Atherosclerosis co-depression of ApoE−/− mice of the prefrontal cortex and hippocampus lipid metabolism pathways of disorder and the changes of to the gut microbiota, which leads to abnormal white matter and synaptic dysfunction, increased gut inflammation, and decreased gut permeability, leading to the release of inflammatory cytokines, there is a strong correlation between both, it further confirmed the existence of the “microbiota-gut-brain axis.”