bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case report: An unusual case of desmin myopathy associated with heart failure and arrhythmia
Authors: Liu Xuhan , Liu Yuan , Li Bo , Wang Lin , Zhang Weihua 
Published: July 2022

IntroductionDesmin myopathy is a novel desmin (DES) indel mutation that causes severe atypical cardiomyopathy as well as atrioventricular block and skeletal myopathy. The mutation of the gene of the nodal tail causes myocardial injury. Rarely does desmin myopathy cause bilateral ventricular changes.Case presentationWe present a case of a 48-year-old man admitted with dyspnea and edema of both lower extremities. Due to bilateral lower limb weakness and calf muscle atrophy, gene sequencing was performed. The results showed that there was a pure missense mutation in the 8th exon region of the DES gene (c.1366G&gt;A), encoding amino acid p.G456R (glycine&gt;arginine). Supplementary examination suggests a high possibility of heart failure, atrial flutter, and desmin myopathy. Atrial flutter was treated by radiofrequency ablation. The clinical symptoms were stable after oral administration of rivaroxaban, coenzyme Q10, and ARNI.ConclusionIn our case, mutation results are the gold standard for the diagnosis of desmin myopathy. Cardiac magnetic resonance can define the extent and degree of cardiomyopathy and quantitatively evaluate cardiac function. At present, there is a lack of specific treatment for proteolytic myopathy. Therefore, the treatment for heart failure proves effective. Due to the multiple systems involved, early diagnosis and multidisciplinary management are critical to improving patient outcomes.
The Chronology of Early Human Settlement in Three Gorges Region, China—Contribution of Coupled Electron Spin Resonance and Uranium-Series Dating Method
Authors: Han Fei , Bahain Jean-Jacques , Shao Qingfeng , Sun Xuefeng , Voinchet Pierre , Xiao Ping , Huang Manchen , Li Mengqi , Yin Gongming 
Published: July 2022

The Three Gorges region (TGR) located in the geographic center of China, is a transition zone between mountain and plain areas, and a probable migration corridor for hominins and other mammals between South and North China. Detailed chronological information of paleoanthropological evidence in this area could help us better understand the human evolution in East Asia. The OSL and U-series dating methods are two conventional dating methods generally adopted to date such sites; however, their applications were limited by the dating range—restricted to several hundred of millennia and ambiguous stratigraphic relationship between the archaeological remains and the dating target materials. Cosmogenic nuclide burial dating of quartzite stone artifacts and coupled electron spin resonance and uranium series dating (ESR/U-series) of fossil teeth have the potential to date Early–Middle Pleistocene hominin sites in Asia and were applied increasingly in China in recent years. However, the application of cosmogenic 26Al/10Be burial dating is limited in TGR because most sites are dominated by limestone, leading to the scarcity of the quartz component. In this case, the coupled ESR/U-series method plays a more important role in the establishment of the chronology of human settlement. In TGR, by using the coupled ESR/U-series method, we have dated seven important Early and Middle Pleistocene hominin settlement sites, including Longgupo, Jianshi, Yunxian, Meipu, Bailongdong, Changyang, and Yumidong sites. Based on our dating results, we propose that hominins were settled in TGR probably from the early stage of Early Pleistocene (∼2.5−2.2 Ma) at the Longgupo site to the late Middle Pleistocene to Late Pleistocene of the Yumidong site (∼274−14 ka) and very likely to spread to other parts of East Asia during this time period. In view of the potential of coupled ESR/U-series dating on fossil teeth from the hominin sites in the TGR, future work may consider the micro damage or non-destructive analysis of enamel fragment with the ESR method and laser ablation ICP-MS techniques that will make possible the direct dating of precious human fossils in China.
Quantitative evaluation of tight oil reservoirs in the Chang 8 Member of the Yanchang Formation in southern Ordos Basin
Authors: Luan Beibei , Zhang Bo , Wang Didong , Deng Chao , Wang Feng 
Published: July 2022

The precise and quantitative characterization of reservoir properties is the key to efficient development of tight oil reservoirs. In this paper, taking the Chang 8 Member of the Yanchang Formation in the Shuimogou area in the southern Ordos Basin as an example, the sedimentary facies types, microscopic pore structures, diagenesis, influencing factors of physical properties, and hydrocarbon enrichment law of tight oil reservoirs are systematically studied. The research results show that the Chang 8 Member in the study area is a typical delta front subfacies deposit, including distributary channel and inter-distributary bay microfacies. The constructive diagenesis of the Chang 8 Member include dissolution, metasomatism and rupture; while the destructive diagenesis include mechanical compaction and cementation. The Chang 8 reservoir has entered the middle diagenetic stage A. The factors affecting the physical properties of tight oil reservoirs include deposition, compaction, cementation and dissolution. The secondary pores formed by dissolution account for 10–40% of the total surface porosity, with an average value of 24%. Local structures and sediments have significant control over hydrocarbon accumulation. The westward dipping tectonic setting of the northern Shaanxi Slope provides the basic conditions for the migration of oil and gas to the eastern updip areas. The changes of lithology and physical properties in the updip direction of the structure form the blocking conditions for the continued migration of oil and gas, which is conducive to the accumulation of oil and gas. In addition, the main oil reservoirs are mainly distributed along the distributary channel of the delta front, and most of the distributary channel sandstone is more than 20 m thick. The areas of thick sand body with multiple layers in the lateral direction is the main part of oil and gas accumulation. However, the reservoirs formed on the flanks of underwater distributary channels are generally thin and poorly sorted, which is not conducive to the accumulation of hydrocarbons.
Petrogenesis of early–middle paleozoic granitoids in the qilianblock, northwest China: Insight into the transition from adakitic to non-adakitic magmatism in a post-collisional extensional setting
Authors: Zhao Jiao-Long , Chen Wan-Feng , Wang Jin-Rong , Tang Qing-Yan , Wang Er-Teng , Fu Yi-Qiao 
Published: July 2022

To better understand geodynamic implications for the transition from adakitic to non-adakitic magmatism in a continental collision setting, an integrated study including zircon U–Pb ages and whole-rock geochemistry was performed for the Gahai and Erhai granitoid intrusions within the Qilian Block. The ca. 450–428 Ma Gahai biotite granite and Erhai muscovite-bearing granite are peraluminous S-type granites with adakitic geochemical characteristics, and have low Mg# (41.2–31.7) and εNd(t) (−10.8 to −6.5) values with two–stage Nd model ages of 2.05–1.72 Ga, implying their derivation almost entirely from an ancient crustal source under a thickened lower crust condition. The geochemical characteristics of the granites indicate that the Gahai and Erhai S-type granites were derived from partial melting of meta-greywacke and meta-pelite sources, respectively. In comparison, the ca. 415 Ma Gahai granodiorite belongs to metaluminous I-type granitoid and has low Sr/Y ratios as well as high Y and heavy rare Earth element (REE) concentrations, with nearly flat heavy REE patterns, implying a shallow source region. The granodiorites were most likely generated by partial melting of a K-rich basaltic magma source, with some contributions from mantle-derived melts. Synthesized data from this and previous studies suggest that the ca. 450–415 Ma Gahai and Erhai granitoids within the Qilian Block were generated in a post-collisional extensional regime triggered by the break-off of the northward subducting South Qilian Ocean slab beneath the Qilian Block. The geochemical transition from adakitic to non-adakitic intermediate-acidic magmas indicated that the thickened continental crust of the Qilian Block had experienced significant extension and thinning after ca. 420 Ma.
Monitoring Surface Deformation Associated With Soil Erosion in Loess Plateau With an Improved Small Baseline Subset Interferometric Synthetic Aperture Radar Algorithm
Authors: Sun Qian , Zhang Heng , Huang Long , He Caihong 
Published: July 2022

As a geomorphic process persistently occurring over the earth’s surface, soil erosion is one of the most serious environmental problems in the world nowadays, seriously threatening agriculture, natural resources, and ecosystem environments. Monitoring surface deformation associated with soil erosion will help to understand the dynamics of erosion process and the erosion mechanism. In this article, an improved small baseline subset interferometric synthetic aperture radar (SBAS-InSAR) technique is proposed to monitor the surface deformation over the wind-water erosion crisscross region in the Loess Plateau, China. In particular, a new deformation model considering both of the internal factors and external factors of soil erosion is introduced into the SBAS-InASR algorithm. The results show that the cumulative deformation is close to −200 mm during the investigated period. The deformation rate caused by precipitation is between −0.03 and 0.02 mm/mm. When the wind erosion factor changes by 1 unit, the deformation range is approximately −0.3–0.2 mm. Also, researchers found that the surface deformation is highly correlated to precipitation and wind. The root mean square errors (RMSEs) of the residual deformations estimated from the proposed model are smaller than those estimated from the conventional linear and period models, demonstrating that the proposed method is more suitable to model and analyze the surface deformation in wind-water erosion crisscross region.
Identification of novel biomarkers in septic cardiomyopathy via integrated bioinformatics analysis and experimental validation
Authors: Lu Feng , Hu Feng , Qiu Baiquan , Zou Hongpeng , Xu Jianjun 
Published: July 2022

Purpose:Septic cardiomyopathy (SCM) is an important world public health problem with high morbidity and mortality. It is necessary to identify SCM biomarkers at the genetic level to identify new therapeutic targets and strategies.Method:DEGs in SCM were identified by comprehensive bioinformatics analysis of microarray datasets (GSE53007 and GSE79962) downloaded from the GEO database. Subsequently, bioinformatics analysis was used to conduct an in-depth exploration of DEGs, including GO and KEGG pathway enrichment analysis, PPI network construction, and key gene identification. The top ten Hub genes were identified, and then the SCM model was constructed by treating HL-1 cells and AC16 cells with LPS, and these top ten Hub genes were examined using qPCR.Result:STAT3, SOCS3, CCL2, IL1R2, JUNB, S100A9, OSMR, ZFP36, and HAMP were significantly elevated in the established SCM cells model.Conclusion:After bioinformatics analysis and experimental verification, it was demonstrated that STAT3, SOCS3, CCL2, IL1R2, JUNB, S100A9, OSMR, ZFP36, and HAMP might play important roles in SCM.
Application of 3D printing individualized guide plates in percutaneous needle biopsy of acetabular tumors
Authors: Wu Wen , Liu Siyu , Wang Lei , Wu Bing , Zhao Lulu , Jiang Wenbo , Dai Kerong , Hao Yongqiang , Fu Lingjie , Ai Songtao 
Published: July 2022

Objective: The objective of the study was to investigate the effectiveness of applying the individualized guide plate which is based on digital image processing and 3D printing technology to percutaneous needle biopsy of periacetabular tumor.Methods: From July 2017 to August 2019, 11 patients (5 males and 6 females, aged 13–70 years, mean 42.3 years) with acetabular tumors diagnosed by needle biopsy in our hospital were enrolled in this retrospective study. Preoperative CT and MRI enhancement examination were performed routinely, and the DICOM data were collected and imported into Medraw Print software. According to the specific anatomical morphology of acetabula, this study adopted the reverse calculation and direct design to print the individualized puncture guide plate using 3D printing technology. The puncture point and sampling approaches were determined by the guide plate morphology and the “double guide-hole and slideable groove” design. First, we evaluated the fitness of the 3D guide plate to the local anatomical structure, its assisted-puncture accuracy was estimated by imaging examinations, and postoperative complications were recorded. The accuracy of the needle biopsy pathological result was estimated with reference to that of the tumor resection.Results: Our results showed that the 3D printing individualized guide plate matched the patients’ pelvic skin well, the puncture approach was consistent with the preoperative design, and no significant anatomical injuries including vascular and neural complications occurred after surgery. Nine patients’ (90%) biopsy results were consistent with their postoperative pathological results, and one patient gave up the tumor resection.Conclusion: Based on digital image processing and 3D printing technology, the individualized guide plate can be used to guide the needle biopsy of acetabular tumors which makes the operation simpler and more precise.
Efficacy and Safety of Neoadjuvant Monoimmunotherapy With PD-1 Inhibitor for dMMR/MSI⁃H Locally Advanced Colorectal Cancer: A Single-Center Real-World Study
Authors: Zhang Xuan , Yang Renfang , Wu Tao , Cai Xinyi , Li Guoyu , Yu Kun , Li Yong , Ding Rong , Dong Chao , Li Jinsha , Hu Ruixi , Feng Qing , Li Yunfeng 
Published: July 2022

ObjectiveTo explore the efficacy and safety of single-agent programmed cell death protein-1 (PD-1) inhibitor in the neoadjuvant treatment of patients with mismatch repair-deficient (dMMR) or microsatellite instability-high (MSI-H) locally advanced colorectal cancer (LACRC) through single-center large⁃sample analysis based on real⁃world data in China.MethodsThis study was a retrospective, single-center, case series study. 33 colorectal cancer (CRC) patients with clinical stage of T3~4N0~2M0 treated in Yunnan Cancer Hospital from June 2019 to June 2021 were analyzed retrospectively. Among them, 32 patients were dMMR or MSI-H or both dMMR and MSI-H, and one patient was both dMMR and microsatellite stability (MSS) (excluded in the final analysis). All 32 patients received neoadjuvant immunotherapy (nIT) with single-agent PD⁃1 inhibitor.ResultsAmong the 32 patients, 8 (25%) were locally advanced rectal cancer (LARC) and 24 (75%) were locally advanced colon cancer (LACC); 4 (12.55%) were stage II and 28 (87.5%) were stage III. The median number of cycles of 32 patients with dMMR/MSI-H LACRC receiving nIT with single-agent PD-1 blockade was 6 (4~10), and the median number of cycles to achieve partial response (PR) was 3 (2~4). Among them, three LARC patients achieved clinical complete response (cCR) and adopted the watch-and-wait (W&amp;W) strategy. The objective response rate (ORR) of the other 29 patients with radical surgery was 100% (29/29), the pathological response rate was 100% (29/29), the rate of major pathological response (MPR) was 86.2% (25/29), and the rate of pathological complete response (pCR) was 75.9% (22/29). The incidence of immune-related adverse events (irAEs) in 32 patients during nIT was 37.5% (12/32), while the incidence of irAEs in 22 patients with operation during adjuvant immunotherapy was 27.3% (6/22), all of which were grade 1~2. No grade 3 or above irAEs were occured. The median time from the last nIT to surgery was 27 (16~42) days. There were no delayed radical resection due to irAEs in these patients. All 29 patients achieved R0 resection. The incidence of surgical-related adverse events (srAEs) in perioperative period was 10.3% (3/29).ConclusionsNeoadjuvant monoimmunotherapy with PD-1 inhibitor has favorable ORR and pCR rate, and relatively low incidences of irAEs and srAEs for patients with dMMR/MSI-H LACRC, suggesting that this nIT regimen of single-agent PD-1 inhibitor is significantly effective and sufficiently safe.
Dysregulation of Subcutaneous White Adipose Tissue Inflammatory Environment Modelling in Non-Insulin Resistant Obesity and Responses to Omega-3 Fatty Acids – A Double Blind, Randomised Clinical Trial
Authors: Fisk Helena L. , Childs Caroline E. , Miles Elizabeth A. , Ayres Robert , Noakes Paul S. , Paras-Chavez Carolina , Antoun Elie , Lillycrop Karen A. , Calder Philip C. 
Published: July 2022

BackgroundObesity is associated with enhanced lipid accumulation and the expansion of adipose tissue accompanied by hypoxia and inflammatory signalling. Investigation in human subcutaneous white adipose tissue (scWAT) in people living with obesity in which metabolic complications such as insulin resistance are yet to manifest is limited, and the mechanisms by which these processes are dysregulated are not well elucidated. Long chain omega-3 polyunsaturated fatty acids (LC n-3 PUFAs) have been shown to modulate the expression of genes associated with lipid accumulation and collagen deposition and reduce the number of inflammatory macrophages in adipose tissue from individuals with insulin resistance. Therefore, these lipids may have positive actions on obesity associated scWAT hypertrophy and inflammation.MethodsTo evaluate obesity-associated tissue remodelling and responses to LC n-3 PUFAs, abdominal scWAT biopsies were collected from normal weight individuals and those living with obesity prior to and following 12-week intervention with marine LC n-3 PUFAs (1.1 g EPA + 0.8 g DHA daily). RNA sequencing, qRT-PCR, and histochemical staining were used to assess remodelling- and inflammatory-associated gene expression, tissue morphology and macrophage infiltration.ResultsObesity was associated with scWAT hypertrophy (P &lt; 0.001), hypoxia, remodelling, and inflammatory macrophage infiltration (P = 0.023). Furthermore, we highlight the novel dysregulation of Wnt signalling in scWAT in non-insulin resistant obesity. LC n-3 PUFAs beneficially modulated the scWAT environment through downregulating the expression of genes associated with inflammatory and remodelling pathways (P &lt;0.001), but there were altered outcomes in individuals living with obesity in comparison to normal weight individuals.ConclusionOur data identify dysregulation of Wnt signalling, hypoxia, and hypertrophy, and enhanced macrophage infiltration in scWAT in non-insulin resistant obesity. LC n-3 PUFAs modulate some of these processes, especially in normal weight individuals which may be preventative and limit the development of restrictive and inflammatory scWAT in the development of obesity. We conclude that a higher dose or longer duration of LC n-3 PUFA intervention may be needed to reduce obesity-associated scWAT inflammation and promote tissue homeostasis.Clinical Trial Registrationwww.isrctn.com, identifier ISRCTN96712688.
Identification and validation of an immune signature associated with EMT and metabolic reprogramming for predicting prognosis and drug response in bladder cancer
Authors: Zhang Zhao , Yu Yongbo , Li Peng , Wang Meilan , Jiao Wei , Liang Ye , Niu Haitao 
Published: July 2022

BackgroundEpithelial-mesenchymal transition (EMT), one leading reason of the dismal prognosis of bladder cancer (BLCA), is closely associated with tumor invasion and metastasis. We aimed to develop a novel immune−related gene signature based on different EMT and metabolic status to predict the prognosis of BLCA.MethodsGene expression and clinical data were obtained from TCGA and GEO databases. Patients were clustered based on EMT and metabolism scores calculated by ssGSEA. The immune-related differentially expressed genes (DEGs) between the two clusters with the most obvious differences were used to construct the signature by LASSO and Cox analysis. Time-dependent receiver operating characteristic (ROC) curves and Kaplan–Meier curves were utilized to evaluate the gene signature in training and validation cohorts. Finally, the function of the signature genes AHNAK and NFATC1 in BLCA cell lines were explored by cytological experiments.ResultsBased on the results of ssGSEA, TCGA patients were divided into three clusters, among which cluster 1 and cluster 3 had completely opposite EMT and metabolic status. Patients in cluster 3 had a significantly worse clinical prognosis than cluster 1. Immune-related DEGs were selected between the two clusters to construct the predictive signature based on 14 genes. High-risk patients had poorer prognosis, lower proportions of CD8+ T cells, higher EMT and carbohydrate metabolism, and less sensitivity to chemotherapy and immunotherapy. Overexpression of AHNAK or NFATC1 promoted the proliferation, migration and invasion of T24 and UMUC3 cells. Silencing ANHAK or NFATC1 could effectively inhibit EMT and metabolism in T24 and UMUC3 cells.ConclusionThe established immune signature may act as a promising model for generating accurate prognosis for patients and predicting their EMT and metabolic status, thus guiding the treatment of BLCA patients.
Evaluation of dietary fish meal analog with or without supplementation of natural feed additives as the substitute of fish meal in juvenile Japanese eel, Anguilla japonica
Authors: Bae Jinho , Lee Seunghyung , Moniruzzaman Mohammad , Hamidoghli Ali , Choi Wonsuk , Lee Seunghan , Min Taesun , Kim Shin-Kwon , Bai Sungchul C. 
Published: July 2022

We investigated the nine experimental diets containing fish meal (FM) and/or fish meal analog (FMA) as the major source of animal protein to determine the optimum FMA level as the substitute of FM protein in the diet of juvenile Japanese eel. In addition, two natural feed additives such as Song-Gang stone (SG) and Yucca meal (YM) were supplemented in the diet to evaluate their efficacy as the immunostimulants. The diets are as follows: 100% FM + 0% FMA in diet (FMA0), 90% FM + 10% FMA in diet (FMA10), 80% FM + 20% FMA in diet (FMA20), 70% FM + 30% FMA in diet (FMA30), 60% FM + 40% FMA in diet (FMA40), FMA0 + 0.4% SG (FMA0SG), FMA0 + 0.1% YM (FMA0YM), FMA20 + 0.4% SG (FMA20SG), and FMA20 + 0.1% YM (FMA20YM). Nine groups of Japanese eel each with three replicates were distributed (initial weight of 9 ± 0.2 g) in rectangular tanks receiving flow through water. Each group of the treatment consisted with 15 fish and fed one of the diets for 8 weeks. At the end of the feeding trial, fish fed with the FMA0 and FMA10 diets showed no significant differences in weight gain (WG), specific growth rate (SGR), feed efficiency (FE), and protein efficiency ratio (PER). Meanwhile, fish fed with FMA20, FMA30, and FMA40 diets showed significantly lower WG, SGR, FE, and PER than the fish fed with the FMA0 (control) diet. In addition, there were no significant differences among fish fed with the SG- and YM-supplemented diet groups. However, lysozyme activities in fish fed with the FMA10, FMA20, FMA30, and FMA40 were significantly lower than the fish fed with the FMA0SG, FMA0YM, FMA20SG, and FMA20YM diets. After 7 days of injection with V. Anguillarum, cumulative survival rates of fish fed with the FMA0SG and FMA0YM diets were significantly higher than the FMA0 diet group. The results revealed that the FMA could replace up to 10% of FM as a protein source in the diet of Japanese eel and both of the natural feed additives (SG and YM) could improve replacing rates of FMA from 10% to 20% without compromising growth and health status of fish.