bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics of plasmid-mediated niche invasion, immunity to invasion, and pheromone-inducible conjugation in the murine gastrointestinal tract
Authors: Hirt Helmut , Greenwood-Quaintance Kerryl E. , Barnes Aaron M. T. , Karau Melissa J. , Till Lisa M. , Palzer Elise , Guan Weihua , VanNieuwenhze Michael S. , Kashyap Purna C. , Patel Robin , Dunny Gary M. 
Published: March 2022

AbstractMicrobial communities provide protection to their hosts by resisting pathogenic invasion. Microbial residents of a host often exclude subsequent colonizers, but this protection is not well understood. The Enterococcus faecalis plasmid pCF10, whose conjugative transfer functions are induced by a peptide pheromone, efficiently transfers in the intestinal tract of mice. Here we show that an invading donor strain established in the gastrointestinal tract of mice harboring resident recipients, resulting in a stable, mixed population comprised of approximately 10% donors and 90% recipients. We also show that the plasmid-encoded surface protein PrgB (Aggregation Substance), enhanced donor invasion of resident recipients, and resistance of resident donors to invasion by recipients. Imaging of the gastrointestinal mucosa of mice infected with differentially labeled recipients and donors revealed pheromone induction within microcolonies harboring both strains in close proximity, suggesting that adherent microcolonies on the mucosal surface of the intestine comprise an important niche for cell-cell signaling and plasmid transfer.
Integrated dual-laser photonic chip for high-purity carrier generation enabling ultrafast terahertz wireless communications
Authors: Jia Shi , Lo Mu-Chieh , Zhang Lu , Ozolins Oskars , Udalcovs Aleksejs , Kong Deming , Pang Xiaodan , Guzman Robinson , Yu Xianbin , Xiao Shilin , Popov Sergei , Chen Jiajia , Carpintero Guillermo , Morioka Toshio , Hu Hao , Oxenløwe Leif K. 
Published: March 2022

AbstractPhotonic generation of Terahertz (THz) carriers displays high potential for THz communications with a large tunable range and high modulation bandwidth. While many photonics-based THz generations have recently been demonstrated with discrete bulky components, their practical applications are significantly hindered by the large footprint and high energy consumption. Herein, we present an injection-locked heterodyne source based on generic foundry-fabricated photonic integrated circuits (PIC) attached to a uni-traveling carrier photodiode generating high-purity THz carriers. The generated THz carrier is tunable within the range of 0–1.4 THz, determined by the wavelength spacing between the two monolithically integrated distributed feedback (DFB) lasers. This scheme generates and transmits a 131 Gbits−1 net rate signal over a 10.7-m distance with −24 dBm emitted power at 0.4 THz. This monolithic dual-DFB PIC-based THz generation approach is a significant step towards fully integrated, cost-effective, and energy-efficient THz transmitters.
A method for quantitatively separating the piezoelectric component from the as-received “Piezoelectric” signal
Authors: Chen Chaojie , Zhao Shilong , Pan Caofeng , Zi Yunlong , Wang Fangcheng , Yang Cheng , Wang Zhong Lin 
Published: March 2022

AbstractPolymer-based piezoelectric devices are promising for developing future wearable force sensors, nanogenerators, and implantable electronics, etc. The electric signals generated by them are often assumed as solely coming from the piezoelectric effect. However, triboelectric signals originated from contact electrification between the piezoelectric devices and the contacted objects can produce non-negligible interfacial electron transfer, which is often combined with the piezoelectric signal to give a triboelectric-piezoelectric hybrid output, leading to an exaggerated measured “piezoelectric” signal. Herein, a simple and effective method is proposed for quantitatively identifying and extracting the piezoelectric charge from the hybrid signal. The triboelectric and piezoelectric parts in the hybrid signal generated by a poly(vinylidene fluoride)-based device are clearly differentiated, and their force and charge characteristics in the time domain are identified. This work presents an effective method to elucidate the true piezoelectric performance in practical measurement, which is crucial for evaluating piezoelectric materials fairly and correctly.
Halogen hydrogen-bonded organic framework (XHOF) constructed by singlet open-shell diradical for efficient photoreduction of U(VI)
Authors: Feng Lijuan , Yuan Yihui , Yan Bingjie , Feng Tiantian , Jian Yaping , Zhang Jiacheng , Sun Wenyan , Lin Ke , Luo Guangsheng , Wang Ning 
Published: March 2022

AbstractSynthesis of framework materials possessing specific spatial structures or containing functional ligands has attracted tremendous attention. Herein, a halogen hydrogen-bonded organic framework (XHOF) is fabricated by using Cl− ions as central connection nodes to connect organic ligands, 7,7,8,8-tetraaminoquinodimethane (TAQ), by forming a Cl−···H3 hydrogen bond structure. Unlike metallic node-linked MOFs, covalent bond-linked COFs, and intermolecular hydrogen bond-linked HOFs, XHOFs represent a different kind of crystalline framework. The electron-withdrawing effect of Cl− combined with the electron-rich property of the organic ligand TAQ strengthens the hydrogen bonds and endows XHOF-TAQ with high stability. Due to the production of excited electrons by TAQ under light irradiation, XHOF-TAQ can efficiently catalyze the reduction of soluble U(VI) to insoluble U(IV) with a capacity of 1708 mg-U g−1-material. This study fabricates a material for uranium immobilization for the sustainability of the environment and opens up a new direction for synthesizing crystalline framework materials.
Balancing the film strain of organic semiconductors for ultrastable organic transistors with a five-year lifetime
Authors: Chen Xiaosong , Wang Zhongwu , Qi Jiannan , Hu Yongxu , Huang Yinan , Sun Shougang , Sun Yajing , Gong Wenbin , Luo Langli , Zhang Lifeng , Du Haiyan , Hu Xiaoxia , Han Cheng , Li Jie , Ji Deyang , Li Liqiang , Hu Wenping 
Published: March 2022

AbstractThe instability of organic field-effect transistors (OFETs) is one key obstacle to practical application and is closely related to the unstable aggregate state of organic semiconductors (OSCs). However, the underlying reason for this instability remains unclear, and no effective solution has been developed. Herein, we find that the intrinsic tensile and compressive strains that exist in OSC films are the key origins for aggregate state instability and device degradation. We further report a strain balance strategy to stabilize the aggregate state by regulating film thickness, which is based on the unique transition from tensile strain to compressive strain with increasing film thickness. Consequently, a strain-free and ultrastable OSC film is obtained by regulating the film thickness, with which an ultrastable OFET with a five-year lifetime is realized. This work provides a deeper understanding of and a solution to the instability of OFETs and sheds light on their industrialization.
Altered expression of the immunoregulatory ligand-receptor pair CD200-CD200R1 in the brain of Parkinson’s disease patients
Authors: Rabaneda-Lombarte Neus , Vidal-Taboada José Manuel , Valente Tony , Ezquerra Mario , Fernández-Santiago Rubén , Martí María José , Compta Yaroslau , Saura Josep , Solà Carme 
Published: March 2022

AbstractNeuroinflammation, in which activated microglia are involved, appears to contribute to the development of Parkinson’s disease (PD). However, the role of microglial activation and the mechanisms governing this process remain uncertain. We focused on one inhibitory mechanism involved in the control of microglial activation, the microglia inhibitory receptor CD200R1, and its ligand CD200, mainly expressed by neurons. The human CD200R1 gene encodes two membrane-associated and two soluble protein isoforms and the human CD200 gene encodes full-length proteins (CD200full) but also truncated (CD200tr) proteins which act as CD200R1 antagonists. Little is known about their expression in the human brain under pathological conditions. We used human peripheral blood monocytes and monocyte-derived microglia-like cells from control subjects to characterize the expression of the CD200R1 mRNA variants, which showed stimulus-specific responses. We provide evidence of increased CD200R1 (mRNA variants and protein isoforms) and CD200 expression (CD200tr mRNA) in brain tissue of PD patients, mainly in the hippocampus, as well as increased CD200 expression (CD200full and CD200tr mRNAs) in iPSCs-derived dopaminergic neurons generated from skin fibroblasts of PD patients. Our results suggest that CD200-CD200R1 signalling is altered in PD, which may affect the microglial function and constitute a potential target in therapeutic strategies for PD.
A global urban microwave backscatter time series data set for 1993–2020 using ERS, QuikSCAT, and ASCAT data
Authors: Frolking Steve , Milliman Tom , Mahtta Richa , Paget Aaron , Long David G. , Seto Karen C. 
Published: March 2022

AbstractUrban settlements are rapidly growing outward and upward, with consequences for resource use, greenhouse gas emissions, and ecosystem and public health, but rates of change are uneven around the world. Understanding trajectories and predicting consequences of global urban expansion requires quantifying rates of change with consistent, well-calibrated data. Microwave backscatter data provides important information on upward urban growth – essentially the vertical built-up area. We developed a multi-sensor, multi-decadal, gridded (0.05° lat/lon) data set of global urban microwave backscatter, 1993–2020. Comparison of backscatter from two C-band sensors (ERS and ASCAT) and one Ku-band sensor (QuikSCAT) are made at four invariant non-urban sites (~3500 km2) to evaluate instrument stability and multi-decadal pattern. For urban areas, there was a strong linear correlation (overall R2 = 0.69) between 2015 ASCAT urban backscatter and a continental-scale gridded product of building volume, across 8450 urban grid cells (0.05° × 0.05°) in Europe, China, and the USA. This urban backscatter data set provides a time series characterizing global urban change over the past three decades.
A neurotechnological aid for semi-autonomous suction in robotic-assisted surgery
Authors: Barragan Juan Antonio , Yang Jing , Yu Denny , Wachs Juan P. 
Published: March 2022

AbstractAdoption of robotic-assisted surgery has steadily increased as it improves the surgeon’s dexterity and visualization. Despite these advantages, the success of a robotic procedure is highly dependent on the availability of a proficient surgical assistant that can collaborate with the surgeon. With the introduction of novel medical devices, the surgeon has taken over some of the surgical assistant’s tasks to increase their independence. This, however, has also resulted in surgeons experiencing higher levels of cognitive demands that can lead to reduced performance. In this work, we proposed a neurotechnology-based semi-autonomous assistant to release the main surgeon of the additional cognitive demands of a critical support task: blood suction. To create a more synergistic collaboration between the surgeon and the robotic assistant, a real-time cognitive workload assessment system based on EEG signals and eye-tracking was introduced. A computational experiment demonstrates that cognitive workload can be effectively detected with an 80% accuracy. Then, we show how the surgical performance can be improved by using the neurotechnological autonomous assistant as a close feedback loop to prevent states of high cognitive demands. Our findings highlight the potential of utilizing real-time cognitive workload assessments to improve the collaboration between an autonomous algorithm and the surgeon.
Balance strategy in hoverboard control
Authors: Shushtari Mohammad , Takagi Atsushi , Lee Judy , Burdet Etienne , Arami Arash 
Published: March 2022

AbstractThis study examines how people learn to perform lower limb control in a novel task with a hoverboard requiring to maintain dynamic balance. We designed an experiment to investigate the learning of hoverboard balance and two control strategies: A hip strategy, which mainly uses hip movements to change the angle of the foot, and an ankle strategy relying more on ankle motion to control the orientation of hoverboard plates controlling the motion. Motor learning was indicated by a significant $$10\pm 4$$

10
±
4

% decrease in the trial completion time (p < 0.001) and a significant 24 ± 11% decrease in total muscle activation (p < 0.001). Furthermore, the participants, who had no prior experience riding a hoverboard, learned an ankle strategy to maintain their balance and control the hoverboard. This is supported by significantly stronger cross-correlation, phase synchrony, lower dynamic time warping distance between the hoverboard plate orientation controlling hoverboard motion, and the ankle angle when compared to the hip angle. The adopted ankle strategy was found to be robust to the foot orientation despite salient changes in muscle group activation patterns. Comparison with results of an experienced hoverboard rider confirmed that the first-time riders adopted an ankle strategy.
Solubilities of CO2, O2 and N2 in rocket propellant 5 under low pressure
Authors: Li Chaoyue , Feng Shiyu , Xu Lei , Peng Xiaotian , Liu Weihua 
Published: March 2022

AbstractThe static method of isochoric saturation was used to measure the solubilities of CO2, O2 and N2 in rocket propellant 5 (RP5) at temperatures ranging from 253.15 to 323.15 K in 10 K intervals and pressures ranging from 0 to 120 kPa. The measurement accuracy of the constructed experimental setup was verified by measuring the solubility of CO2 in water. The relative expanded uncertainty (k = 2) in the solubility data was less than 4.0%. The solubilities of CO2, O2 and N2 in RP5 increased with pressure. As the temperature increased, the solubility decreased for CO2 solubility and increased for O2 and N2. Henry's constants for the three gases in RP5 decreased over the experimental temperature and pressure ranges in the order of N2 > O2 > CO2. The measured solubilities of CO2, O2 and N2 could be fitted with a modified Krichevsky–Kasarnovsky equation, and the maximum deviation between the measured and calculated data was less than 8.04%, 7.03% and 6.18%, respectively.
EEG theta and N400 responses to congruent versus incongruent brand logos
Authors: Dini Hossein , Simonetti Aline , Bigne Enrique , Bruni Luis Emilio 
Published: March 2022

AbstractNeuroimaging and behavioral studies have shown that brands convey meaning to consumers. To investigate the immediate reactions of the brain to brand logos, followed either by congruent or incongruent pictorial brand-related cues, can deepen understanding of the semantic processing of brands, and perhaps how consolidated the logo is in consumers’ minds. Participants were exposed to different brand-related image sets, that were either congruent (a match between brand-related images and brand logo) or incongruent (a mismatch between brand-related images and brand logo) while having their brain signals recorded. Event-related potential and EEG time–frequency domain features were extracted from the signals of the target image (brand logo). The results showed significantly larger N400 peak and relative theta power increase for incongruent compared to congruent logos, which could be attributed to an error-monitoring process. Thus, we argue that brands are encoded deeply in consumers’ minds, and cognitive processing of mismatched (vs matched) brand logos is more difficult, leading to greater error monitoring. The results were mostly consistent with previous studies investigating semantic incongruences in the linguistic field. Therefore, the error-monitoring process could be extended beyond linguistic forms, for example to images and brands.
Navigation of frameless fixation for gamma knife radiosurgery using fixed augmented reality
Authors: Moon Hyeong Cheol , Park Sang Joon , Kim Young Deok , Kim Kyung Min , Kang Ho , Lee Eun Jung , Kim Min-Sung , Kim Jin Wook , Kim Yong Hwy , Park Chul-Kee , Kim Young Gyu , Dho Yun-Sik 
Published: March 2022

AbstractAugmented reality (AR) offers a new medical treatment approach. We aimed to evaluate frameless (mask) fixation navigation using a 3D-printed patient model with fixed-AR technology for gamma knife radiosurgery (GKRS). Fixed-AR navigation was developed using the inside-out method with visual inertial odometry algorithms, and the flexible Quick Response marker was created for object-feature recognition. Virtual 3D-patient models for AR-rendering were created via 3D-scanning utilizing TrueDepth and cone-beam computed tomography (CBCT) to generate a new GammaKnife Icon™ model. A 3D-printed patient model included fiducial markers, and virtual 3D-patient models were used to validate registration accuracy. Registration accuracy between initial frameless fixation and re-fixation navigated fixed-AR was validated through visualization and quantitative method. The quantitative method was validated through set-up errors, fiducial marker coordinates, and high-definition motion management (HDMM) values. A 3D-printed model and virtual models were correctly overlapped under frameless fixation. Virtual models from both 3D-scanning and CBCT were enough to tolerate the navigated frameless re-fixation. Although the CBCT virtual model consistently delivered more accurate results, 3D-scanning was sufficient. Frameless re-fixation accuracy navigated in virtual models had mean set-up errors within 1 mm and 1.5° in all axes. Mean fiducial marker differences from coordinates in virtual models were within 2.5 mm in all axes, and mean 3D errors were within 3 mm. Mean HDMM difference values in virtual models were within 1.5 mm of initial HDMM values. The variability from navigation fixed-AR is enough to consider repositioning frameless fixation without CBCT scanning for treating patients fractionated with large multiple metastases lesions (> 3 cm) who have difficulty enduring long beam-on time. This system could be applied to novel GKRS navigation for frameless fixation with reduced preparation time.
Detection and identification of potentially infectious gastrointestinal and respiratory viruses at workplaces of wastewater treatment plants with viability qPCR/RT-qPCR
Authors: Stobnicka-Kupiec Agata , Gołofit-Szymczak Małgorzata , Cyprowski Marcin , Górny Rafał L. 
Published: March 2022

AbstractThis study aimed to qualitatively and quantitatively assess the prevalence of the most common respiratory and gastrointestinal viruses in the air, surface swab, and influent/effluent samples collected in wastewater treatment plants (WWTPs). Application of qPCR/RT-qPCR (quantitative polymerase chain reaction/reverse-transcription quantitative polymerase chain reaction) assays combined with PMA (propidium monoazide) dye pretreatment allowed detecting the potentially infectious and disintegrated viral particles in collected samples. In the air at workplaces in WWTPs, the most frequent isolation with the highest concentrations (reaching up to 103 gc/m3 of potentially infectious intact viral particles) were observed in case of adenoviruses (AdVs) and rotaviruses (RoVs), followed by noroviruses (NoVs). Viruses were significantly more often detected in the air samples collected with Coriolis μ impinger, than with MAS-100NT impactor. The temperature negatively (Spearman correlation: –1 < R < 0; p < 0.05), while RH (relative humidity) positively (0 < R < 1; p < 0.05) affected airborne concentrations of potentially infectious viral particles. In turn, the predominant viruses on studied surfaces were RoVs and noroviruses GII (NoV GII) with concentrations of potentially infectious virions up to 104 gc/100 cm2. In the cases of SARS-CoV-2 and presumptive SARS-CoV-2 or other coronaviruses, their concentrations reached up to 103 gc/100 cm2. The contamination level of steel surfaces in WWTPs was similar to this on plastic ones. This study revealed that the qualitative and quantitative characteristics of respiratory and gastrointestinal viruses at workplaces in WWTPs is important for proper exposure assessment and needs to be included in risk management in occupational environment with high abundance of microbial pollutants derived from wastewater.
Insight into redox regulation of apoptosis in cancer cells with multiparametric live-cell microscopy
Authors: Shirmanova Marina V. , Gavrina Alena I. , Kovaleva Tatiana F. , Dudenkova Varvara V. , Zelenova Ekaterina E. , Shcheslavskiy Vladislav I. , Mozherov Artem M. , Snopova Ludmila B. , Lukyanov Konstantin A. , Zagaynova Elena V. 
Published: March 2022

AbstractCellular redox status and the level of reactive oxygen species (ROS) are important regulators of apoptotic potential, playing a crucial role in the growth of cancer cell and their resistance to apoptosis. However, the relationships between the redox status and ROS production during apoptosis remain poorly explored. In this study, we present an investigation on the correlations between the production of ROS, the redox ratio FAD/NAD(P)H, the proportions of the reduced nicotinamide cofactors NADH and NADPH, and caspase-3 activity in cancer cells at the level of individual cells. Two-photon excitation fluorescence lifetime imaging microscopy (FLIM) was applied to monitor simultaneously apoptosis using the genetically encoded sensor of caspase-3, mKate2-DEVD-iRFP, and the autofluorescence of redox cofactors in colorectal cancer cells upon stimulation of apoptosis with staurosporine, cisplatin or hydrogen peroxide. We found that, irrespective of the apoptotic stimulus used, ROS accumulation correlated well with both the elevated pool of mitochondrial, enzyme-bound NADH and caspase-3 activation. Meanwhile, a shift in the contribution of bound NADH could develop independently of the apoptosis, and this was observed in the case of cisplatin. An increase in the proportion of bound NADPH was detected only in staurosporine-treated cells, this likely being associated with a high level of ROS production and their resulting detoxification. The results of the study favor the discovery of new therapeutic strategies based on manipulation of the cellular redox balance, which could help improve the anti-tumor activity of drugs and overcome apoptotic resistance.