bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of IP10/CXCR3 signaling with highly coincidental with PrPSc deposit in the brains of scrapie infected mice
Authors: chen jia , Chen Cao , Hu Chao , Yang Wei , Wang Lin , Chen Dong-Dong , Wu Yue-Zhang , Shi Qi , Dong Xiao-Ping 
Published: November 2022

AbstractActivation of chemokine IP10, also named as CXCL10, and its receptor CXCR3 in CNS is described in some neurodegenerative diseases. Our previous study has also demonstrated an increased brain IP10 levels in several scrapie infected rodent models. However, the detailed alteration of IP10/CXCR3 signaling in CNS during prion infection remains unsettled. Here, we found the increased IP10 signals in the brains of scrapie infected mice mainly localized in the neurons using various methodologies. The levels of CXCR3 were markedly increased in brains of the scrapie infected mice and in the prion infected cell line SMB-S15. The increased CXCR3 is mainly distributed in neurons and activated microglia. Obviously morphological colocalizations of PrPC/PrPScwith IP10 and CXCR3 in the brains of scrapie infected mice were observed in the assays of immunohistochemistry (IHC) and immunofluorescence. Additionally, IHC analysis with whole brain sections demonstrated that the increased IP10 and CXCR3 accumulations occurred in the brain regions with more PrPScdeposits. Co-immunoprecipitation and biomolecular interaction assays identified the evidence for the molecular interactions of PrP with IP10 and CXCR3. Compared to the normal partner cell line SMB-PS, a larger amount of IP10 accumulated inside prion infected SMB-S15 cells. Suppression of prion replication in SMB-S15 cells by added resveratrol reverted the pattern of accumulation and secretion of cellular IP10 to that observed in SMB-PS cells. Our data here demonstrate an activation of IP10/CXCR3 signaling in prion-infected brain tissues that coincide with deposited PrPSc. Modulation of brain IP10/CXCR3 signaling is potential therapeutic target for reducing the progression of prion diseases.
Can social media reach isolated domestic abuse victims? Evidence from a randomized control trial during the Covid-19 lockdown.
Authors: Grogger Jeffrey , Ivandić Ria , Kirchmaier Tom 
Published: November 2022

Abstract
Research Questions
Can social media reach isolated domestic abuse victims? Secondly, does providing victims with more information and a safer means of contacting police change their likelihood of domestic abuse reporting?
Data
This research is based on high frequency and confidential administrative data on the population of domestic abuse calls during the period of the Covid-19 pandemic but also the preceding years from two police forces – the Thames Valley Police (TVP) and the Metropolitan Police Service (MPS).
Methods
To answer the research questions, we ran a randomized control trial (RCT) using a novel social media campaign promoting a method of reporting through Facebook and Instagram. We randomised the treatment across geographic areas in one police force and across individuals in another police force.
Findings
We found that while social media is an effective tool for engaging on domestic abuse topics, particularly with younger individuals, our intention-to-treat estimates between the treatment and control areas and individuals did not show any significant difference in domestic abuse reporting. One of the reasons to explain this finding was the geographically imprecise social media targeting features on Facebook.
Conclusions
Our research contributes to the scarce experimental literature on how to increase domestic abuse reporting among victims with, to the best of our knowledge, the first randomised test of the effects of a social media campaign on engagement and reporting. As police forces across the United Kingdom, but also worldwide, start using social media more to engage with the citizens they serve, these results provide interesting and valuable implications for their effectiveness and the role of technology in the future policing.
Performance improvement of photothermal membrane distillation with a Ti/MgF2 photoabsorber enhanced robustness by plasma pretreatments
Authors: Song Kyung Guen , Cho Jinsoo , Wang Dasom , Choi Jihyeok , Mo Jeongeun , Choi Won Jun , Baik Jung Min , Jang Ho Won , Jung Jinho 
Published: November 2022

Abstract
To meet the increasing global freshwater demand, desalination processes are a promising alternative technology for future water supply. Solar membrane distillation is a forward-looking desalination process, and it can minimize the use of electrical energy. We developed a photothermal membrane with high thermal performance and durability to reduce the energy loss caused by feed circulation for solar heating and reduce the environmental pollution caused by photothermal materials in the solar desalination process. The developed Ti/MgF2 membrane showed an absorbance of 83.3% of AM 1.5G at wavelengths of 280-2500 nm. The Ti/MgF2 membrane pretreated with Ar plasma exhibited a maximum temperature of 62.8°C and a temperature increase of approximately 40.5% compared to the unpretreated photothermal membrane under the 1 sun condition. Using the developed membrane, the water flux was improved by up to 26.9%, the performance ratio (PR) by 35.4%, and the thermal efficiency by 50.8% under the 1 sun condition. The produced permeate maintained high drinkability quality with a salt rejection rate of 99.9% or more. The developed photothermal membrane shows potential for application to membrane distillation (MD) using solar heat.
Higher Education Students’ Propensity to Embrace and Use New Technology Based Services: An Empirical Study
Authors: Jore Shubhangi , Asthana Nidhi 
Published: November 2022

Abstract
Understanding and analysing the consumers with respect to their adoptability of technology – based services, the time and process through which these services are accepted and practiced is of much importance. There is need to understand all the drives and insights that may influence customers’ technology readiness. As the requirements of technology ready customers will ultimately affect the strategies that are implemented to develop new services. What people are thinking about new technology, their beliefs about the same and advancement in the technology is need of the hour. It has become very important to analyse people’s belief about technology so that further their predispositions to use it and prediction of their perception can be identified and analysed. In recent years the advancement in the Indian technology sector in terms of smartphones and laptops is seeing tremendous competitiveness, growth, efficiency, profitability and soundness. By comparing the technology readiness of some of the widely used electronic gadgets such as smartphones and laptops, we can find out how fast people are accepting new technology. The purpose of this paper is to test the validity of Technology Readiness Index (TRI) (Parasuraman, 2000) with respect to management students in Indore city. Present study is an attempt to identify the factors that affect adoption of technology-based services amongst higher education students. To explore whether there is any significant classification of higher education students with respected to the factors affecting adoption of technology-based services. The study also aims at exploring how demographics variables may help to explain adoption and use of technology-based products and services. A sample of size 200 respondents was collected though non- probabilistic convenient sampling using the standard scale on Technology Readiness Index (Parasuraman, 2000). Multivariate data analysis techniques were applied to analyse the data. The results support the TRI validity in Indore city. The study also suggests that demographic variables play an important role in predicting pro-technological behaviour in the city. This will enable both businesses and government to develop strategies for new services based on advanced technology.
Noble gas isotopic characteristics in Paleo-Salt Lakes brine of the western Qaidam Basin
Authors: Li Hongpu , Pan Tong , Jin Fang , Chen Jinniu , Guo Ruirui , Tong Yongjun 
Published: November 2022

Abstract
The brine in paleo-salt lakes in the Qaidam Basin is a very important resource of potassium and lithium salts. There has been little research on the formation mechanism of the brine according to noble gases and their isotopic characteristics, let alone research on the formation age of the brine. For this reason, this paper studies the formation mechanism and formation age of the brine in paleo-salt lakes in the Qaidam Basin according to the test data of the samples of noble gas elements and their isotopes collected in the latest deep boreholes in the Qaidam Basin. In the study area, the He content in the brine ranges from 12.2×10-6 to 1,326.9×10-6, the Ne content from 1.253×10-6 to 19.1×10-6, and the Ar content from 3,046.5×10-6 to 5,023.1×10-6; the ratio of 3He/4He ranges from 2.00 ×10-8 to 2.30×10-7, R/Ra from 0.01 to 0.16Ra, 20Ne/22Ne from 9.2 to 10.6, 21Ne/22Ne from 0.024 to 0.032, and 40Ar/36Ar from 298.7 to 352. In combination with the correlations between 20Ne/22Ne and 21Ne/22Ne, and 3He/4He and 40Ar/36Ar, and in accordance with the special physical properties of noble gases He, Ne, and Ar (such as lower solubility in groundwater and higher solubility with the increase of ion radius), the following can be inferred: The brine in paleo-salt lakes in the Qaidam Basin has two sources: deep crust and atmospheric precipitation; the reservoirs of the brine in paleo-salt lakes in the Qaidam Basin are Quaternary - Paleogene strata; the brine was formed from Triassic to Ordovician, earlier than the reservoir age according to the calculation of 3He/4He in the brine based on the age accumulation effect.
Interventions to Improve Provider Competence in Low-and-Middle-Income Countries (LMICs): A Scoping Review
Authors: Salloum Caitlin , Feil Cameron , Ramadan Marwa , Uribe Manuela Villar 
Published: November 2022

Abstract
Background: Despite a global movement to strengthen primary health care systems, quality of care often remains poor across low-and-middle-income countries. A key determinant for quality of care is provider competence. This scoping review specifically aims to build on the Health Care Provider Performance Review (HCPPR), a systematic review of interventions to improve health care performance in low-and-middle-income countries, by identifying the approaches, effect, and characteristics of successful provider competence interventions at the PHC level.
Methods: PubMed, Scopus, and Embase were used to search for peer-reviewed publications and grey literature that reported evidence of interventions to improve provider competence in PHC settings in LMICs.
Results: A total of 37 articles met the inclusion criteria. Three themes were identified: education and training, supervision and mentorship, and protocols and tools. Most studies included in the review took place in Sub-Saharan Africa (60 %) and in lower-middle income countries (57 %). Among the 37 articles, 27 (73 %) utilized an education and training intervention, 18 (48 %) utilized a supervision and mentoring intervention, and 15 (41 %) utilized a protocol and tool intervention. A total of 19 (51%) interventions were multicomponent, containing more than one intervention theme. Most interventions reported positive outcomes, although few presented evidence on the long-term impacts of the interventions. Among the three themes, education and training interventions reported more successful interventions compared to supervision and mentoring or protocol and tool-based interventions. Multicomponent interventions reported the most favorable outcomes compared to independent interventions.
Conclusion: Provider competence interventions, when appropriately planned, implemented, and sustained are effective for improving the skills, knowledge, and competence of providers in LMICs. When feasible, interventions that combine multiple approaches consistently show to have the greatest short- and long-term effects on the competence of providers. However, despite the available research, there is still a lack of evidence on adapting, implementing, and sustaining interventions to improve provider competence across contexts.
JAG-1/Notch signaling axis contributes to the maintenance of bone cancer pain in female rats via spinal astrocyte-neuron crosstalk
Authors: 伏 杰 , Zhao Baoxia , Ni Huadong , Xu Longsheng , Luo Ge , He Qiuli , Xu Miao , Xu Chengfei , Wang Yahui , Ni Chaobo , Kong Min , Shou Qiyang , Yao Ming 
Published: November 2022

Abstract
Background
Recent studies have shown that interactions between astrocytes and neurons in the spinal cord are involved in chronic pain. In this study, we investigated whether the Jagged-1(JAG-1) /Notch signaling pathway regulates bone cancer pain (BCP) via the astrocyte-neuron mechanism.
Methods
Walker256 breast cancer cells were injected into the tibia bone marrow of the female rat to establish a stable BCP rat model. The molecular mechanism of hyperalgesia mediated by the JAG-1/Notch signal pathway was determined by western blotting, immunofluorescence, real-time quantitative polymerase chain reaction, dual-luciferase reporter gene, and chromatin immunoprecipitation assays. Von-Frey test, catwalk gait analysis, open field test, and conditioned place aversion test were used to study changes in pain behavior in rats. In addition, the effect of the JAG-1/Notch signal pathway on neuronal excitability was also investigated.
Results
The intramedullary injection of Walker256 breast cancer cells induces persistent hyperalgesia and increased the expression of JAG-1, Notch intracellular domain (NICD), and c-Fos in the spinal cord. The expression of JAG-1 was exclusively observed in astrocytes and Notch-1 was expressed only in neuronal cells. Astrocyte activation increased JAG-1 expression, and knockdown of JAG-1 in the spinal cord reduced BCP. The supplementation of exogenous JAG-1 to the spinal cord induced BCP-like behavior and promoted expression of c-Fos and hairy and enhancer of split homolog-1 (Hes-1) in the spinal cord of the naïve rats. These effects were reversed when the rats were administered intrathecal injections of DAPT, an inhibitor of notch signaling. The intrathecal injection of DAPT reduced BCP and inhibited Hes-1 and c-Fos expression in the spinal cord. Furthermore, our results showed that JAG-1 regulated neural excitability via the Notch signaling pathway. JAG-1 upregulated Hes-1 expression by inducing the recruitment of NICD to the RBP-J/CSL binding site located within the Hes-1 promoter sequence. Finally, the intrathecal injection of c-Fos-ASO and administration of sh-Hes-1 to the spinal dorsal horn also alleviated BCP.
Conclusion
JAG-1/Notch signaling axis mediated interaction of astrocyte-neuron contributes to the maintenance of bone cancer-induced pain hypersensitivity. The inhibition of the crosstalk between astrocytic JAG-1 and neuronal Notch-1 may serve as a potential strategy for the treatment of BCP.
Osteocytes exposed to titanium particles inhibit osteoblastic cell differentiation via connexin 43/β-catenin pathway
Authors: Chai Hao , Huang Qun , Jiao Zixue , Wang Shendong , Sun Chunguang , Geng De Chun , Xu Wei 
Published: November 2022

AbstractBackground Periprosthetic osteolysis (PPO) induced by wear particles is the most severe complication of total joint replacement, however, the mechanism of PPO remains unknown. Previous studies have shown that osteocytes play an important role in wear particle-induced osteolysis. In this study, we investigated the effects of connexin 43 (Cx43) on the regulation of osteocyte to osteoblast differentiation. Methods In vivo, a murine model of calvarial osteolysis induced by titanium (Ti) nanoparticles was established. The osteolysis characteristic and osteogenesis markers in the osteocyte-selective deficiency of Cx43 (cKO) mice and wild-type (WT) mice were observed. In vitro, osteocytic cell line MLO-Y4 was treated with Ti nanoparticles. β-catenin were detected when Cx43 of MLO-Y4 cell was silenced or overexpressed. Co-culture of MLO-Y4 cells with MC3T3-E1 osteoblastic cells was used to observe the effects of Ti-treated osteocyte on osteoblast differentiation. Co-immunoprecipitation detection of Cx43 and β-catenin binding in MLO-Y4 cells and MC3T3-E1 cells. Results In vivo, the calvarial osteolysis induced by Ti particles was partially attenuated in the cKO mice. The expression of β-catenin, Runx2, osterix, alkaline phosphatase (ALP), and osteocalcin (OCN) increased significantly in the femurs of cKO mice. In vitro. Ti particles decreased β-catenin expression and increased Cx43 expression. Silencing of Cx43 increased the β-catenin expression in MLO-Y4 cells and over-expressed Cx43 decreased the β-catenin expression. In the co-culture model, as a result of Cx43 silencing in the MLO-Y4 cells, the MC3T3-E1 cells displayed decreased Cx43 expression, increased β-catenin expression, activation of Runx2, and promotion of osteoblastic differentiation. Cx43 silencing in MLO-Y4 cells attenuated the inhibitory effects of the Ti treatment of MLO-Y4 cells on the osteoblastic differentiation of MC3T3-E1 cells. Finally, Cx43 expression was found to be negatively correlated to the activity of the Wnt signaling pathway through binding of β-catenin to the nucleus. Conclusion In conclusion, the results of our study suggest that Ti nanoparticles increased Cx43 expression in osteocytes and osteocytes may participate in the regulation of osteoblast function via the Cx43/β-catenin pathway during periprosthetic osteolysis.
“Isolation and Characterization of Phosphofungi – Aspergillus niger from Rhizosphere Soil to supplement Phospho-biofertilizer”
Authors: V Jyothi , Basaiah Thippeswamy 
Published: November 2022

Abstract
In the present study, the phosphate-solubilizing fungus was isolated and identified as Aspergillus niger. Its 18S rRNA gene sequence was deposited at GenBank, NCBI (MN904862). For the study of the Phosphate solubilization capacity of the fungus, different rhizosphere soil samples were collected from medicinal plants. 40 fungal colonies were isolated after serial dilution and one fungal colony with high phosphate solubilization zone was selected and further tested. The fungus showed good results in different phosphate solubilization tests, SI (3.91), SE (290), and pH of the culture filtrate after the growth of the fungus decreased from 6.89 to 3.13 due to the production of organic acids. The colour changed from blue to yellow on the agar plate and red to yellow in the broth due to the acidic condition of the media during the growth of the fungus. Titrable acidity was measured at 32.8g/L and 420µg of P was estimated in culture broth by the Vanadomolybdate method. The fungus showed a positive result for siderophore production. 20µg of Indole Acetic Acid (IAA) was produced by the fungus and was estimated by the Salkowaski reagent method using a standard calibration curve. Due to the phosphate solubilization capacity and production of plant growth promoters of A. niger, can be recommended as a Phosphate solubilizer in an agricultural field.
A prediction method for the uniaxial compressive strength of the soil-rock mixture considering the effect of the particle size
Authors: Zhang Zhenping , Fu Xiaodong , Yuan Wei , Zhou Yongqiang , Huang Juehao , Sheng Qian , Chai Shaobo 
Published: November 2022

Abstract
The reasonable prediction of mechanical parameters of the soil-rock mixture (S-RM) is crucial to build an engineering structure on the deposit slope. As a typical multiphase geomaterial, the emergence of rock blocks of various sizes destroys the integrity of the soil matrix and results in the complex and varied mechanical properties because of its interaction with the soil matrix. Because of the size effect, which is caused by the size restriction of traditional test apparatus, it is more challenging to measure the precise mechanical parameters of S-RM. This study proposed an approach to predicting the uniaxial compressive strength (UCS) of S-RM taking the particle size effect into account. The impact of the particle size on the mechanical behaviors of S-RM under various volumetric rock block proportion (VBP) conditions was discussed using the particle flow discrete element method from both a macroscopic and mesoscopic view. The parameter “Am” was used to describe the decreasing rate of UCS with various VBPs, and a quantitative description to predict the parameter “Am” of S-RM with various maximum particle sizes was proposed. A method to predict the UCS value of S-RM with various VBPs was conducted while taking the influence of size effect into account using the parameter “Am” and the UCS of the soil matrix.
Collagen III deposition and basement membrane integrity alterations: possible predictive markers in metastatic prostate cancer
Authors: Pinheiro Laís Capelasso Lucas , Pupim Andreia Carla Eugenio , Tomeleri Marilia Folini , Pereira Érica Romão , Francelino Amanda Letícia , Araújo Eduardo José Almeida , Guembarovski Alda Fiorina Maria Losi , Fuganti Paulo Emilio , Colus Ilce Mara de Syllos , Miqueloto Carlos Alberto , Guembarovski Roberta Losi 
Published: November 2022

Abstract
Purpose
Metastasis represents the major cause of deaths in cancer patients, and the tumor surrounding extracellular matrix (ECM) passes through changes in its organization during the evolution of this process. Therefore, the aim of the present study was to quantify the deposition of proteins that constitute the ECM, namely total collagen, collagen I (Col I) and collagen III (Col III) in samples from patients with metastatic (mPCa) and non-metastatic prostate cancer (PCa), in addition of evaluating the basement membrane integrity.
Methods
Tissue samples from 60 patients were divided into three groups according to parameters ISUP grade, TNM staging and PSA concentration: better prognosis (n = 20), worse prognosis (n = 23) and metastatic (n = 17). To quantify collagen, the Picrosirius Red technique was used with further analysis under a polarization microscope, and to basement membrane analysis the Periodic Acid Schiff (PAS) technique was employed, where the coloring was classified in G1, G2 and G3.
Results
It was observed that the Col I/ Col III ratio was higher in the metastatic group in relation to better prognosis (p = 0.012) and worse prognosis (p = 0.018) groups. About the basement integrity, it was observed that its constitution in the malignant tumor tissue differed from the adjacent non-tumor tissue (p = 0.000). Also, the worsening in the tumor tissue integrity was positively correlated with worse prognosis parameters (advanced ISUP grade, extraprostatic extension and perineural invasion).
Conclusion
Our study indicates that the absence of Col III can constitute a marker for potential metastatic tumors. The basement membrane integrity also seems to be an indicator of poor prognosis in malignant prostatic tumors.