bluuebunny/crossref_metadata_2025_split huggingface dataset:
2H1C Mimicry: Bioinspired Iron and Zinc Complexes Supported by N,N,O Phenolate Ligands
Authors: Monkcom Emily C. , Negenman Hidde A. , Masferrer‐Rius Eduard , Lutz Martin , Ye Shengfa , Bill Eckhard , Klein Gebbink Robertus J. M. 
Published: February 2022

AbstractIn pursuit of mimicking the ubiquitous 2H1C motif in mononuclear non‐heme iron enzymes, two new bioinspired N,N,O phenolate ligands, BenzImNNO and ImPh2NNO, are synthesised and their coordination chemistry with zinc(II) and iron(II) is explored. BenzImNNO coordinates by means of an anionic κ3‐N,N,O donor set and readily forms homoleptic bisligated complexes, also in the presence of equimolar amounts of metal salt. In contrast, the increased steric bulk of ImPh2NNO promotes the formation of dinuclear complexes, [M2(ImPh2NNO)2(OTf)2] (M=Fe, Zn), with facially opposing metal sites, as a result of its unique bridging μ2:κ2‐N,N:κ1‐O coordination mode. We investigate the robustness of the ligand's dinucleating coordination mode during oxidative transformations and demonstrate that its coordination mode is retained upon triflate substitution for a biorelevant thiophenolate co‐ligand.
Metal‐free phthalimide‐labeled peptide nucleic acids for electrochemical biosensing applications
Authors: Magni Mirko , Dall'Angelo Sergio , Baldoli Clara , Licandro Emanuela , Falciola Luigi , Mussini Patrizia R. 
Published: February 2022

AbstractPeptide nucleic acids (PNAs) are neutral mimics of natural DNA and RNA biopolymers that have caught the attention of researchers working on the identification of specific sequences of nucleobases in DNA/RNA strands. For this purpose, specific analytical protocols need to be developed to optimize the nucleic acid recognition ability of PNAs, exploiting both the high intrinsic affinity of PNA/DNA(RNA) couples as well as suitable markers, either linked to the PNA backbone or properly interacting with it in the working medium. In this context, the paper reports on phthalimide and 4‐nitrophthalimide as two cheap, metal‐free electroactive markers covalently bound to the pseudo‐peptide backbone of a PNA decamer. After a preliminary characterization of the markers, as such and in PNA conjugates, attention has been moved toward the optimization of the detectability of the labeled PNA decamers in aqueous solutions. Exploiting the potentiometric stripping analysis on hanging mercury drop electrode it has been possible to reach satisfactory detection limits of ca. 10 nM avoiding the use of expensive transition metal complexes as labels and/or of co‐reagents.
Identification of the promising olive (Olea europaea L.) cultivars based on morphological and pomological characters
Authors: Khadivi Ali , Mirheidari Farhad , Moradi Younes , Paryan Simin 
Published: February 2022

AbstractOlive (Olea europaea L.) is an ancient tree and can tolerate drought very well. In the present study, morphological and pomological diversity of 24 olive cultivars (5–15 replications for each cultivar, 243 trees in total) was evaluated. There were significant differences among the cultivars studied based on the characters recorded. The CV was more than 20.00% in 46 of 50 characters measured. Leaf length ranged from 27.07 to 78.54 mm, and leaf width varied from 5.42 to 23.06 mm. Ripening date ranged from late‐August to early‐October. Fruit length ranged from 13.04 to 33.72 mm, fruit diameter varied from 10.24 to 23.71 mm, fruit weighted from 0.97 to 9.61 g, and the range of fruit flesh thickness was 1.63–7.65 mm. There was high variability in terms of fruit color, ranging from light green to black. Hierarchical cluster analysis (HCA) performed based on the mean of replications with Euclidean distance and Ward method grouped the cultivars into two major clusters. Differences in many of the morphological traits were observed across the cultivars. These sets of data were used to identify unique and desirable cultivars morphologically. The present research demonstrates that local olive cultivars have unique characteristics that differentiate them from imported cultivars. Thus, local cultivars provide novel genetic resources that should be conserved.
Machine learning directed sentinel lymph node biopsy in cutaneous head and neck melanoma
Authors: Oliver Jamie R. , Karadaghy Omar A. , Fassas Scott N. , Arambula Zack , Bur Andrés M. 
Published: February 2022

AbstractBackgroundThe specificity of sentinel lymph node biopsy (SLNB) for detecting lymph node metastasis in head and neck melanoma (HNM) is low under current National Comprehensive Cancer Network (NCCN) treatment guidelines.MethodsMultiple machine learning (ML) algorithms were developed to identify HNM patients at very low risk of occult nodal metastasis using National Cancer Database (NCDB) data from 8466 clinically node negative HNM patients who underwent SLNB. SLNB performance under NCCN guidelines and ML algorithm recommendations was compared on independent test data from the NCDB (n = 2117) and an academic medical center (n = 96).ResultsThe top‐performing ML algorithm (AUC = 0.734) recommendations obtained significantly higher specificity compared to the NCCN guidelines in both internal (25.8% vs. 11.3%, p < 0.001) and external test populations (30.1% vs. 7.1%, p < 0.001), while achieving sensitivity >97%.ConclusionMachine learning can identify clinically node negative HNM patients at very low risk of nodal metastasis, who may not benefit from SLNB.
Real‐world use of palbociclib monotherapy in retroperitoneal liposarcomas at a large volume sarcoma center
Authors: Nassif Elise F. , Cope Brandon , Traweek Raymond , Witt Russell G. , Erstad Derek J. , Scally Christopher P. , Thirasastr Prapassorn , Zarzour Maria Alejandra , Ludwig Joseph , Benjamin Robert , Bishop Andrew J. , Guadagnolo B. Ashleigh , Ingram Davis , Wani Khalida , Wang Wei‐Lien , Lazar Alexander J. , Torres Keila E. , Hunt Kelly K. , Feig Barry W. , Roland Christina L. , Somaiah Neeta , Keung Emily Z. 
Published: February 2022

AbstractPalbociclib has been evaluated in early phase trials for well‐differentiated liposarcoma (WDLPS) and dedifferentiated liposarcoma (DDLPS) patients, with reported median progression‐free survival (PFS) of 18 weeks. Here, we report on real‐world use and surgical outcomes associated with palbociclib treatment. We retrospectively reviewed 61 consecutive patients with retroperitoneal WDLPS (n = 14) or DDLPS (n = 47) treated with palbociclib monotherapy between 1 March 2016 and 28 February 2021 at The University of Texas MD Anderson Cancer Center. At palbociclib initiation, median age was 64 (interquartile range [IQR] 56‐72). In WDLPS and DDLPS cohorts, the median number of prior systemic treatments was 0 (IQR 0‐0) and 2 (IQR 0‐4), respectively. Median number of prior surgeries was 2 (WDLPS IQR 1‐2.75) and 2 (DDLPS IQR 1‐3). Median PFS was 9.2 (WDLPS IQR 3.9‐21.9) and 2.6 months (DDLPS IQR 2.0‐6.1), with median time on treatment of 7.4 months (WDLPS IQR 3.5‐14.2) and 2.7 months (DDLPS IQR 2.0‐5.7). Twelve patients ultimately underwent surgical resection. Resections were macroscopically complete (R0/R1) in half (n = 6/12), among whom only one patient experienced relapse after resection (median follow‐up 7.5 months). All patients who underwent macroscopically incomplete resections progressed after surgery with median time to progression of 3.3 months (IQR 2.3‐4.4). Surgery after palbociclib treatment was not associated with improved overall survival. Efficacy of palbociclib monotherapy for patients with advanced WDLPS and DDLPS is disappointing. While palbociclib may have been used to delay surgery, there was no clear benefit from treatment and few patients achieved prolonged tumor control.
Apelin‐13/APJ induces cardiomyocyte hypertrophy by activating the Pannexin‐1/P2X7 axis and FAM134B‐dependent reticulophagy
Authors: Yang Yiyuan , Zhang Kai , Huang Shifang , Chen Wei , Mao Hui , Ouyang Xueqian , Chen Linxi , Li Lanfang 
Published: February 2022

AbstractCardiac hypertrophy is a leading cause of cardiac morbidity and mortality worldwide. Apelin is the endogenous ligand for the G protein‐coupled receptor, APJ. Previously, we have revealed that apelin‐13 can induce cardiomyocyte hypertrophy by activating the autophagy pathway. However, the precise mechanism through which apelin‐13 regulates reticulophagy to participate in cardiomyocyte hypertrophy remains unclear. Herein, we observed that apelin‐13‐induced cardiomyocyte hypertrophy by activating FAM134B‐dependent reticulophagy via the Pannexin‐1/P2X7 signal pathway. Furthermore, we found that apelin‐13 stimulated the opening of Pannexin‐1 hemichannel and increased extracellular ATP (eATP) levels, which activated the P2X7 purinergic receptor. Activation of the Pannexin‐1/eATP/P2X7 axis subsequently led to FAM134B‐dependent reticulophagy. Moreover, inhibition of the Pannexin‐1/P2X7 axis and FAM134B‐dependent reticulophagy reversed apelin‐13‐induced cardiomyocyte hypertrophy. Based on our present findings, apelin‐13/APJ induces cardiomyocyte hypertrophy by activating the Pannexin‐1/P2X7 axis and FAM134B‐dependent reticulophagy.
Exosomal microRNAs and long noncoding RNAs: Novel mediators of drug resistance in lung cancer
Authors: Taghvimi Sina , Vakili Omid , Soltani Fard Elahe , Khatami Seyyed Hossein , Karami Neda , Taheri‐Anganeh Mortaza , Salehi Mahsa , Negahdari Babak , Ghasemi Hassan , Movahedpour Ahmad 
Published: February 2022

AbstractLung cancer therapeutic resistance, especially chemoresistance, is a key issue in the management of this malignancy. Despite the development of novel molecularly targeted drugs to promote therapeutic efficacy, 5‐year survival of lung cancer patients is still dismal. Molecular studies through the recent years have fortunately presented multiple genes and signaling pathways, which contribute to lung cancer chemoresistance, providing a better perception of the biology of tumor cells, as well as the molecular mechanisms involved in their resistance to chemotherapeutic agents. Among those mechanisms, transfer of extracellular vesicles, such as exosomes, between cancer cells and the surrounding noncancerous ones is considered as an emerging route. Exosomes can desirably function as signaling vesicles to transmit multiple molecules from normal cells to cancer cells and their microenvironment, or vice versa. Using this ability, exosomes may affect the cancer cells' chemoresistance/chemosensitivity. Recently, noncoding RNAs (esp. microRNAs and long noncoding RNAs), as key molecules transferred by exosomes, have been reported to play a substantial role in the process of drug resistance, through modulation of various proteins and their corresponding genes. Accordingly, the current review principally aims to highlight exosomal micro‐ and long noncoding RNAs involved in lung cancer chemoresistance. Moreover, major molecular mechanisms, which connect corresponding RNA molecules to drug resistance, will briefly be addressed, for better clarifying of possible roles of exosomal noncoding RNAs in promoting the effectiveness of lung cancer therapy.
Prospective, Multi‐Center Study of the Anatomic Distribution of Recurrent Respiratory Papillomatosis
Authors: Benedict Peter A. , Kravietz Adam , Achlatis Efstratios , Wang Binhuan , Zhang Yan , Kidane Joseph , Harrison Tina , Miller Jonas , Drake Virginia E. , Best Simon R. , McWhorter Andrew J. , Lin R. Jun , Rosen Clark A. , Smith Libby J. , Amin Milan R. 
Published: February 2022

Objectives/HypothesisTo create a model of the anatomic distribution, recurrence, and growth patterns of recurrent respiratory papillomatosis (RRP).Study DesignProspective, multi‐institutional cohort study.MethodsAdult patients with a diagnosis of RRP evaluated between August 1, 2018 and February 1, 2021 at six participating centers were invited to enroll. At each office or operating room encounter, laryngologists recorded the location and size of RRP lesions using a 22‐region schematic. A generalized linear mixed effects model was used to compare region variations in lesion prevalence and recurrence.ResultsThe cohort comprised 121 patients: 74% were male, 81% had been diagnosed with adult‐onset RRP, and a plurality (34%) had undergone 0 to 3 RRP interventions prior to enrollment. Across the study period, the odds of a lesion occurring in the glottis was significantly higher (odds ratio [OR]: 26.51; 95% confidence interval [CI]: 11.76–59.75, P < .001) compared with all other areas of the larynx and trachea. Within the true vocal folds, the membranous vocal folds had significantly higher odds (OR: 6.16; 95% CI: 2.66–14.30, P < .001) of lesion occurrence compared to the cartilaginous vocal folds. Despite these strong trends in lesion distribution, there were no differences in the odds of lesion recurrence, growth, or in the time to recurrence, between anatomic subsites.ConclusionsRRP lesions are most likely to occur in the glottis, particularly the membranous vocal folds, compared with other regions of the larynx or trachea. However, all lesions demonstrate similar behavior with respect to recurrence, growth, and time to recurrence regardless of anatomic location.Level of Evidence3 Laryngoscope, 132:2403–2411, 2022
A novel 8.57‐kb deletion of the upstream region of PRKAR1A in a family with Carney complex
Authors: Ito Shin , Hashimoto Aya , Yamaguchi Kazunori , Kawamura Sadafumi , Myoen Shingo , Ogawa Maki , Sato Ikuro , Minato Takamichi , Miyabe Shingo , Nakazato Akira , Fujii Keitaro , Mochizuki Mai , Fujimori Haruna , Tamai Keiichi , Niihori Tetsuya , Aoki Yoko , Sugawara Akira , Sasano Hironobu , Shima Hiroshi , Yasuda Jun 
Published: February 2022

AbstractCarney complex (CNC) is a rare hereditary syndrome that involves endocrine dysfunction and the development of various types of tumors. Chromosome 2p16 and PRKAR1A on chromosome 17 are known susceptibility loci for CNC. Here we report a mother and son with CNC caused by an 8.57‐kb deletion involving the transcription start site and non‐coding exon 1 of PRKAR1A. The proband is a 28‐year‐old male with bilateral large‐cell calcified Sertoli cell testicular tumors and pituitary adenoma. Comprehensive genomic profiling for cancer mutations using Foundation One CDx failed to detect any mutations in PRKAR1A in DNA from the testicular tumor. Single‐nucleotide polymorphism array analysis of the proband’s genomic DNA revealed a large deletion in the 5′ region of PRKAR1A. Genomic walking further delineated the region an 8.57‐kb deletion. A 1.68‐kb DNA fragment encompassed by the deleted region showed strong promoter activity in a NanoLuc luciferase reporter assay. The patient’s mother, who is suffering from recurrent cardiac myxoma, a critical sign for CNC, carried an identical deletion. The 8.57‐kb deleted region is a novel lesion for CNC and will facilitate molecular diagnosis of the disease.
Development of mobile health–based self‐management support for patients with lung cancer: A stepwise approach
Authors: Ni Xiaosha , Lou Yan , Hu Wenyi , Wang Hemei , Xu Hong , Li Shuaini , Zhou Yunxian , Ni Yisha 
Published: February 2022

AbstractAimTo develop self‐management support platform on mobile for Chinese patients with lung cancer.DesignA stepwise approach with combination of methods.MethodsLiterature review was carried out to construct preliminary framework and develop detailed content for self‐management modules. A semistructured interview was conducted to elicit preference in the content and platform of self‐management modules with 15 patients with lung cancer. Delphi study was performed to evaluate and improve the content of modules with 13 experts. A pilot study was conducted to test the mobile health–based self‐management support modules with 13 patients with lung cancer.ResultsA social network software‐based WeChat public account “Symptom Self‐Management” for patients with lung cancer was developed and preliminarily tested with positive feedback. Three modules were designed and presented, including symptom management (cancer‐related fatigue, cough, dyspnoea, pain, nausea and vomiting), emotion management and role management.ConclusionThis study showed that patients with lung cancer have diversified supportive care needs after discharge. A bottom‐up and stepwise approach to develop mobile health–based self‐management support tool has shown to be feasible and valuable. Theory guidance, user requirement exploration, evidence‐based information and expert evaluation are key elements of the process.Implications for PracticeThe WeChat Public Account “Symptom Self‐Management” could be used as sustainable platform to support patients with lung cancer in dealing with common challenges. It provides professional information, self‐assessment tools, self‐management skills and peer‐support platform. Information presented in both text and audio forms enables patients' easy access to the platform.
Exercise interventions for weight management during pregnancy and up to 1 year postpartum among normal weight women and women with overweight and obesity: An updated systematic review
Authors: Hanley Stephanie J. , Sibbick Elise , Varley Ian , Sale Craig , Elliott‐Sale Kirsty J. 
Published: February 2022

AbstractBackgroundGiven the increased occurrence of pre‐gravid obesity in recent years, and the implications of maternal obesity for maternal and offspring health, it is evident that there is a continued need to investigate antenatal and postnatal weight management strategies and to provide evidence‐based advice for exercise‐based interventions. Given the small number of studies (n = 5) included in an original systematic review by our group in 2015, it was important to add to the dataset by assessing data published since 2015, in order to provide a more in‐depth view of current knowledge.ObjectiveTo provide an updated systematic review on studies employing exercise interventions for weight management in pregnant and postpartum women.MethodsA systematic review of randomised controlled trials evaluating the effects of an exercise intervention on gestational weight gain and postpartum weight management in normal weight women, and women with overweight and obesity was conducted. PubMed, Scopus, Central Register of Controlled Trials and Web of Science were searched for studies published between September 2013 and June 2021. No restrictions were set on type, intensity, duration, or frequency of exercise intervention. Only studies that targeted body weight or mass as a primary outcome were included.ResultsThirteen studies were included in this review: 11 during and two following pregnancy. Exercise significantly reduced gestational weight gain in five of the pregnancy studies and induced significant weight loss in one of the postpartum studies. Across studies, there were large disparities in exercise modality, frequency, and duration, although moderate intensity exercise was consistently employed.ConclusionsSome studies showed positive effects of exercise on weight management during and following pregnancy. While there is still no consensus on effective exercise intervention approaches, it is crucial that physical activity, of any safe form, is recommended to encourage healthy weight management during this time.
Factors associated with postoperative hypocalcemia following thyroidectomy in childhood
Authors: Spinelli Claudio , Ghionzoli Marco , Bertocchini Alessia , Sanna Beatrice , Plessi Carlotta , Strambi Silvia , De Napoli Luigi , Frustaci Gianluca , Materazzi Gabriele , Antonelli Alessandro , Morganti Riccardo , Gennari Fabrizio , Inserra Alessandro , Bisogno Gianni , Gamba Piergiorgio , Ferrari Andrea , Massimino Maura 
Published: February 2022

AbstractBackgroundPostoperative hypocalcemia is a frequent complication after thyroidectomy. Hypoparathyroidism may develop as transient (TtHP), with normalization within six months from surgery, or permanent (PtHP) if the patient requires replacement therapy. We analyzed factors associated with the development of postoperative hypoparathyroidism and in detail PtHP following thyroid surgery in a pediatric population.ProcedureA retrospective multicenter study analyzing 326 patients was carried out. We recorded gender, age, tumor size, thyroiditis, extrathyroidal extension, lymph node dissection (central/lateral compartment, unilateral/bilateral), parathyroid autotransplantation, and histology. Additionally, calcium levels were acquired postoperatively.ResultsWe analyzed pediatric patients ≤18 years who underwent thyroidectomy clustered into age groups (≤15 or > 15). Patients’ mean follow‐up was 5.8 years (1‐11 years). Postoperative hypoparathyroidism occurred in 36 (11.0%): 20 cases (6.13%) developed PtHP. Postoperative hypoparathyroidism was more frequent in younger patients (P = 0.014), in larger tumors (P < 0.001), in case of extrathyroidal extension (P = 0.037), and in central compartment (P = 0.020) and bilateral lymph node dissection (P = 0.030). PtHP was more frequent in older patients (P = 0.014), in case of thyroiditis (P < 0.001), and extrathyroidal extension (P < 0.001). Concerning the first postoperative calcium level measurement, in the postoperative hypoparathyroidism group, we registered a 8.17 mg/dL value with 14% pre/postoperative decrease (ΔCa), whereas in PtHP patient group calcium level was 7.91 mg/dL with 16.7% ΔCa.ConclusionsThe risk of postoperative hypoparathyroidism is related to younger age, tumor size, central compartment and bilateral lymph node dissection, extrathyroidal extension, and decrease in postoperative calcium levels. The risk of PtHP is related to older age, thyroiditis, extrathyroidal extension, and decrease in postoperative calcium levels.
The determination of monosaccharide in different years Qingzhuan Dark Tea polysaccharide by liquid chromatography–mass spectrometry
Authors: Miao Xiao‐Lei , Ma Hui‐Min , Ke Qin‐Hao , Wang Shi‐Yue , Zhou Hong‐Fu , Zheng Min 
Published: February 2022

AbstractAimTo establish a fast, sensitive and accurate high‐performance liquid chromatography‐tandem mass spectrometry (HPLC‐MS/MS) method for determining the monosaccharide content of Qingzhuan Dark Tea polysaccharides in different years (2 years, 5 years and 11 years).MethodsThe optimised chromatographic conditions were achieved on a C18 column (5.0 μm, 250 mm × 4.6 mm inner diameter). The mobile phase flow rate was 0.9 mL/min and the column temperature was set to 27°C. The aqueous phase A (5 mM aqueous ammonium acetate) and organic phase B (acetonitrile) were used to elute the target analyses isocratically (0–60 min: 18% B). The mass spectrometer detector was equipped with an electron spray ionisation (ESI)source, and multiple reaction monitoring (MRM) mode was used for the determination of 1‐phenyl‐3‐methyl‐5‐pyrazolone (PMP) derived monosaccharides.ResultsWe carried out a comprehensive methodological validation of PMP derived monosaccharides, including linearity, precision, stability and repeatability. Nine monosaccharides (rhamnose, mannose, ribose, glucose, galacturonic acid, xylose, galactose, fucose and arabinose) of Qingzhuan Dark Tea polysaccharides were identified, in which ribose and fucose were reported for the first time. The results showed the contents of these nine monosaccharides differed significantly among different years.ConclusionsThe validated method is reliable, accurate, repeatable and can be applied to quality assessment of these monosaccharides.