bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytotoxic Effects of Coated Gold Nanoparticles on PC12 Cancer Cell
Authors: Zare Marzouni Hadi , Tarkhan Fazel , Aidun Amir , Shahzamani Kiana , Jahan Tigh Hamid Reza , Malekshahian Sareh , Esmaeil Lashgarian Hamed 
Published: January 2022

Background: The use of gold nanoparticles in medicine and especially in cancer treatment has been of interest to researchers. The effectiveness of this nanoparticle on cells significantly depends on the amount of its entry into the cells. This study was performed to compare the rate and mechanism of effect of gold nanoparticles coated with different amino acid on PC12 cancer cell line.Materials and Methods: The PC12 cells line were exposed to various concentrations of amino acid coated and uncoated gold nanoparticles (0.5, 2.5 and 5 μM). Cell death rate was determined according to level of Lactate dehydrogenase (LDH) release from cells and MTT assay. In addition cell morphology and the amount of Cellular Reactive oxygen species (ROS) were studied.Results: The uncoated gold nanoparticles have shown minor effects on cellular life. Gold nanoparticles coated by tryptophan at high concentrations (2.5, 5 and 25μM) increase in cancer cells metabolic activity. Gold nanoparticles coated by Aspartate also produce the largest amount of LDH and ROS in cancer cells and therefore caused of highest rate of apoptosis.Conclusion: The results showed that the nanoparticles coated with amino acids are affected on cellular metabolism and apoptosis more than uncoated nanoparticles. Also the smallest coated nanoparticles (coated by aspartate) have the most influence and by increasing the size, this effect was reduced. [GMJ.2018;7:e1110]
Prediction of Leishmania Major Key Proteins via Topological Analysis of Protein-Protein Interaction Network
Authors: Amiri Dashatan Nasrin , Rezaie Tavirani Mostafa , Zali Hakimeh , Koushki Mehdi , Ahmadi Nayebali 
Published: January 2022

Background: Although leishmaniasis is regarded as a public health problem, no effective vaccine or decisive treatment has been introduced for this disease. Therefore, representing novel therapeutic proteins is essential. Protein-protein Interaction network analysis is a suitable tool to discover novel drug targets for leishmania major. To this aim, gene and protein expression data is used for instructing protein network and the key proteins are highlighted.Materials and Methods: In this computational and bioinformatics study, the protein/gene expression data related to leishmania major were studied, and 252 candidate proteins were extracted. Then, the protein networks of these proteins were explored and visualized by using String database and Cytoscape software. Finally, clustering and gene ontology were performed by MCODE and PANTHER databases, respectively.Results: Based on gene ontology analysis, most of the leishmania major proteins were located in cell compartments and membrane. Catalytic activity and binding were regarded as the relevant molecular functions and metabolic and cellular processes were the significant biological process. In this network analysis, UB-EP52, EF-2, chaperonin, Hsp70.4, Hsp60, tubulin alpha and beta chain, and ENOL and LACK were introduced as hub-bottleneck proteins. Based on clustering analysis, Lmjf.32.3270, ENOL and Lmjf.13.0290 were determined as seed proteins in each cluster.Conclusion: The results indicated that hub proteins play a significant role in pathogenesis and life cycle of leishmania major. Further studies of hubs will provide a better understanding of leishmaniasis mechanisms. Finally, these key hub proteins could be a suitable and helpful potential for drug targets and treating leishmaniasis by considering their validation. [GMJ.2018;7:e1129]
Association Between First Episode Schizophrenia, Metabolic Syndrome and Insulin Resistance-Related Proteins in Female Balb/C Mice
Authors: Sattar Haseeb , Li Huqun , Han Yong , Zhou Hong , Darbalaei Sanaz , Li Weiyong 
Published: January 2022

Background: Metabolic syndrome is a group of different disorders mainly includes, insulin resistance, obesity, cerebrovascular disorders, dyslipidemia, which leads to increase mortality. Patients suffering from related psychotic disorders such as schizophrenia are at the higher risk of developing metabolic syndrome. The aim of this study was to evaluate the association between the first episode of schizophrenia, metabolic syndrome and insulin resistance-related proteins in blood and adipose tissue of mice.Materials and Methods: Twelve, female Balb/c mice were randomly divided into two groups; one group was injected intraperitoneal MK-801 (0.6mg/kg/d) to induce schizophrenia, and other group received the 0.9% normal saline for two weeks. Body weight, fasting blood glucose (FBG), oral glucose tolerance (OGT), and Homeostatic model assessment (HOMA), were observed. Blood and adipose tissue were collected and Western blotting was done to evaluate the insulin resistance related proteins (GGPPS, FAT, PTP-1B, GRK2, ATGL, FGF21, and PGC-1α) by using GAPDH as an internal standard. Results: There was a significant increase in mean body weight in schizophrenic group (21.76 vs 22.81, P=004). On day 14, the FBG, insulin concentrations and Homeostatic model assessment and insulin resistance (HOME-IR) were high in schizhphrenic group vs control group, e.g. 5.3±0.6 vs 3.47±0.2 (P=0.0001), 28.9±2.2 vs 23.3±0.6 (P<0.005) and 9.2±1.3 vs 3.9±0.2 (P=0.0001) . Impaired glucose tolerance deranged from 4.8mmol/L to 6.4mmol/L. Western blotting showed a marked increase in the expression of GGPPS, FAT, ATGL, and FGF21 proteins in monocytes and PTP-1B, GRK2, and PGC-1α ratios in adipose tissues.Conclusion: There was a positive relation between schizophrenia and metabolic syndrome e.g. insulin resistance and obesity. Certain proteins in adipocytes and blood were responsible for causing insulin resistance. [GMJ.2018;7:e692]
Вплив дапагліфлозину на активність ангіотензин-перетворюючого ферменту в структурах гіпоталамо-гіпофізарно- адренокортикальної системи та функцію кори надниркових залоз у щурів із цукровим діабетом 2-го типу
Authors: Kalynska L.M. , Kovzun O.I. 
Published: January 2022

Мета роботи — дослідити вплив інгібітору натрійзалежного котранспортера глюкози 2 (НЗКТГ2) — дапагліфлозину (ДГФ) на активність ангіотензин-перетворюючого ферменту (АПФ) у центрах регуляції гіпоталамо-гіпофізарно-адренокортикальної системи (ГГАС), а також рівень кортикостероїдів та активність АПФ у сироватці крові щурів із цукровим діабетом 2-го типу (ЦД2). Матеріал і методи. ЦД2 в щурів самців моделювали за допомогою висококалорійної дієти (ВКД) та ін’єкцій низьких доз стрептозотоцину (15 і 25 мг/кг). Суспензію ДГФ вводили щурам із ЦД2 per os у дозі 1 мг/кг один раз на добу впродовж 8 діб. Активність АПФ в структурах ГГАС (гіпоталамусі, гіпофізі та надниркових залозах) і сироватці крові щурів визначали флуориметричним методом із використанням субстрату Benzoyl-Gly-His-Leu («Sigma», США). Вміст 11-гідроксикортикостероїдів (11-ОКС) у сироватці крові щурів вимірювали флуориметричним мікрометодом. Результати. Показано, що в щурів на ранній стадії розвитку ЦД2 (через 2 тижні після порушення толерантності до глюкози та підвищення рівня базальної глікемії) активність АПФ у структурах ГГАС і в сироватці крові не відрізняєтьсявід активності ферменту в контролі. У нейрогіпофізі щурів із двотижневим ЦД2 на відміну від структур ГГАС виявлено суттєве підвищення рівня білкових і пептидних речовин. У сироватці крові щурів із ЦД2 виявлено підвищення рівня 11-ОКС. Введення щурам із ЦД2 ДГФ протягом 8 днів призводить до зниження ктивності АПФ та рівня 11-ОКС у сироватці крові. У надниркових залозах цих тварин активність АПФ має тенденцію до зниження. Дослідження ефекту введення ДГФ у центральних ланках ГГАС виявили значне підвищення активності АПФ в аденогіпофізі щурів із ЦД2. Висновки. На ранній стадії розвитку ЦД2 АПФ не приймає участь у порушенні функції ГГАС — змін активності ферменту в гіпоталамусі, аденогіпофізі та надниркових залозах не виявлено. Підвищення рівнів білкових і пептидних речовин у нейрогіпофізі щурів на ранній стадії ЦД2 свідчить про посилення біосинтезу нанопептидних гормонів, зокрема окситоцину і вазопресину. Наслідком прямої дії цих гормонів, очевидно, є виявлене нами підвищення секреторної активності надниркової ланки ГГАС, зокрема рівня кортикостероїдів. Зниження активності сироваткового АПФ та рівня кортикостероїдів після 8-денного введення ДГФ щурам із ЦД2 можуть бути важливими чинниками, які забезпечують зниження інсулінорезистентності, формування якої посилює ангіотензинова система, а також сприяють зниженню артеріального тиску, що позитивно впливає на функції нирок та систему кровообігу.
KARAKTERISTIK PEMUKIMAN KUMUH KAMPUNG KANDANG AYAM
Authors: Fitriyati Dika , Sari Suzanna Ratih 
Published: January 2022

Abstract: Kandang Ayam settlement in lowland typology and directly adjacent to the river, and vulnerable to flood area. In evolution, this village became a place who migrants hope to get a job. Migrants who do not have a permanent residence, and then they build some0house0and no give attention of environmental aspects, and it can give impression of0slums.0The0purpose0of0thisFstudyRisVtoLfind out the characteristicsRof0slumsUinRKandang Ayam village. Analytical method used is qualitative analysis with steps : (1) Analyze and describe by used the theory of factors causingLenvironmentalTslum based on theory (2) Assesement based on ministerial regulation oftpubliciworkiandipublicihousinginumberl2 of 2016 on quality improvement oftslumRhousingRand slums. ResultToftthiststudyNisethevcharacteristicsvofislumsiin Kandang Ayam village at the moderate level of slums with characteristics, as follows : water network, drainage and sanitation quality are poor, in thistvillagetmajority of the house is homes not liveable and makes impression of slum.NThetsocio-economic conditionsioftthetpopulationtintgenerally low income, unfortunately motivation to have a decent and healthy home is still low.Abstrak: Permukiman kampung Kandang Ayam secaraRtipologiRberadaRpada kawasanmdatarannrendahndannberbatasanilangsungidenganisungai, dan termasuk dalam daerah rawan bencana banjir. Dalam perkembangannya kampung ini menjadi lokasi tempat tinggal para pendatang yang berharap dapat memperoleh pekerjaan. Warganpendatangiyang tidak memiliki tempat tinggal tetap, dan mereka membangun tempat tinggal secarawmandirintanpanmemperhatikannaspek-aspektlingkungan, hal tersebutlah yang kemudian dapat menimbulkan kesan kumuh. Tujuannpenelitiannini yaituMuntuknmengetahuinkarakteristiknpemukimanmkumuhRdinkampungnkandang ayam.tMetodetanalisisiyangidigunakantadalahtmetodeikualitatiftdengantlangkah: (1) Menganalisis dan mendeskripsikan menggunakan teori faktor faktor penyebab kekumuhanRlingkunganRberdasarkan teori analisis; (2) Melakukan penilaian menggunakan Peraturan Menteri PUPR Nomor 2 Tahun 2016 tentang peningkatan kualitas terhadap perumahan kumuh dan permukiman kumuh. Hasil dari penelitian ini ialah karakteristik darinpermukimannkumuhndi kampungnKandang Ayam memiliki tingkattkekumuhantsedang, dengan karakteristik sebagai berikut : jaringan air bersih, sistem drainase dan sanitasi yangnburuk,mMayoritas rumah termasuk rumah tidak layak huni dan terkesan kumuh.RKondisiMsosialMekonomiNpenduduknumumnya berpenghasilanirendahisehinggaimotivasi untuk memiliki rumah yang layak dan sehat masih rendah
PENGARUH LOKASI BANGUNAN TERHADAP BENTUK DAN KONSTRUKSI RUMAH TRADISIONAL DI JAWA BARAT
Authors: Harapan Andi 
Published: January 2022

Abstract:. West Java has so many topographical area, such as highland (mountain), low land (valley) and coastal area. All of these areas have differently influenced the typology of building system, which coming from their knowledge of local material, cultures, and technology, which have been delivered from their generation. The knowledge has been developed by times with trial and error process and become the local knowledge of this area (specific area). This paper will be elaborating the buildings (houses) on this 3 topographical area, with focusing on 3 systems of the building (house): lower, upper, and middle system. 3 parts of these building have been observed and created to propose the mapping of construction joint system. On this paper, the map of contraction system (which related to the area) has been explored on 3 traditional villages in West Java: Panjalin village at Majalengka District (located at coastal area), Cikondang village at Bandung District (located at highland area), and Mahmud Village at Bandung District (located at lowland area).Abstrak: Jawa Barat mempunyai geografis yang beragam, terdiri dari area pantai, dataran tinggi (pegunungan) dan dataran rendah. Kondisi geografis yang beragam ini, dihuni oleh masyarakat Jawa Barat yang memberikan ciri khas tersendiri dimana kehidupan tersebut berada, khususnya untuk masyarakat perkampungannya. Uniknya di Jawa Barat terdapat berbagai jenis bangunan tradisional yang mencirikan masyarakatnya, misalnya bangunan tradisional masyarakat di pantai, bangunan tradisional masyarakat di dataran rendah, dan bangunan tradisional masyarakat di dataran tinggi (pegunungan). Untuk itu, pada tulisan ini dilakukan pemetaan bangunan tradisional yang mewakili ketiga area tersebut, dengan melakukan pengamatan dan pengukuran bangunan. Pemetaan dilakukan pada 3 (tiga) lokasi yaitu kampung Panjalin–kabupaten Majalengka (yang mewakili area pantai), Kampung Cikondang–Kabupaten Bandung (yang mewakili area dataran tinggi (pegunungan), dan kampung Mahmud–Kabupaten Bandung (yang mewakili area dataran rendah). Setiap area mempunyai keunikan tersendiri, tetapi juga mempunyai persamaan, seperti konfigurasi bangunan, bentuk fisik bangunan, material, dll, tetapi ada juga perbedaan diadalam dimensi dan beberapa detail bangunan.
Leucaena leucocephala, White Leadtree
Authors: Andreu Michael G. , Friedman Melissa H. , Northrop Robert J. 
Published: January 2022

Leucaena leucocephala, White Leadtree (FOR299/FR367)
In Florida, white leadtree is a prohibited species and therefore is not used in commercial applications in the state. However, in its native range it is used as a source of charcoal, fuel, and lumber. It has also been planted as a windbreak for crops such as coffee and cocoa, and some ranchers use the tree as a source of both shade and forage for cattle, with the pods being an excellent source of protein. In addition, as white leadtree forms a well-developed taproot, it has been planted to assist with erosion control. This 2-page fact sheet was written by Michael G. Andreu, Melissa H. Friedman, and Robert J. Northrop, and published by the UF Department of School of Forest Resources and Conservation, July 2012.
http://edis.ifas.ufl.edu/fr367
PENGARUH PEMBERIAN MICROORGANISME LOKAL KEONG MAS TERHADAP PERTUMBUHAN SERTA HASIL PRODUKSI BAWANG MERAH VARIETAS LOKANANTA
Authors: Yusmina Wori Hana  , Uska Peku Jawang  , Suryani Kurniawi K. L. Kapoe  
Published: January 2022

Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian microorganisme lokal keong mas terhadap pertumbuhan serta hasil produksi tanaman bawang merah (Allium ascalonicum L.). Penelitian dilaksanakan dilahan Kelompok Tani Marangga Hamu Kelurahan Malumbi,Kecamatan Kambera Kabupaten Sumba Timur pada bulan Juni sampai September 2021. Rancangan Acak Lengkap metode yang digunakan dalam penelitian ini, dengan 6 perlakuan yaitu Po (tanpa pemberian POC MOL keong mas), P1 (50 ml/ polibag), P2 (100 ml/ polibag), P3 (150 ml/ polibag), P4 (200 ml/polibag), P5 (250 ml/ polibag) serta masing-masing perlakuan diulang 4 kali. Hasil yang diperoleh bahwa pupuk organik cair keong mas sangat berpengaruh nyata terhadap terhadap jumlah daun bawang merah varietas lokananta dan tidak berpengaruh signifikan terhadap jumlah umbi serta berat kering umbi. Dosis terbaik yang meningkatkan pertumbuhan dan hasil tanaman bawang merang yaitu 250ml/polibag atau 177 liter/ ha.
Analysis of the results of epizootic monitoring of viral diarrhea — a disease of mucous membranes in cattle in the Russian Federation according to the reporting of 4-vet for 2020
Authors: Mikhailova V. V. , Lobova T. P. , Shishkina M. S. , Skvortsova A. N. 
Published: January 2022

Relevance. The reproductive health of dairy cows is one of the top priorities in dairy farming. Viral diarrhea, a disease of mucous membranes in cattle, is one of the causes of impaired reproductive function on farms. The disease is widespread throughout the world and causes significant economic damage, which consists of a decrease in productivity, lack of offspring, impaired reproductive function of the broodstock, costs of treatment and specific prevention. The viral diarrhea virus has an immunosuppressive effect. Diagnosis is complicated by a “blurry” clinical picture due to the association of the virus with other respiratory-intestinal viruses and the addition of a secondary bacterial infection, as well as the presence of a persistent form of infection. The diagnosis requires the analysis of clinical and epizootic data, pathological changes and a combination of several methods of laboratory diagnostics. An important link in the prevention and recovery of herds is the conduct of epizootic monitoring and analysis of its results.Methodology. The assessment of the epizootic situation for viral diarrhea, a disease of mucous membranes in cattle in the Russian Federation for 2020, was carried out based on the results of the analysis of information obtained from annual reports provided by state veterinary laboratories in the FSBI CNMVL in the form of 4-vet (annual).Results. Based on the results of the epizootic monitoring, it can be concluded that the viral diarrhea virus is widely circulating in the livestock farms of the Russian Federation. In 2020, positive results were recorded in all federal districts of the Russian Federation (in 33 subjects), except for the Far Eastern Federal District. The most difficult epizootic situation is in the North Caucasus Federal District and the Central Federal District (38% and 29% of the total number of positive results, respectively).
ANALISIS SIFAT FISIS AGREGAT HALUS PASIR DAN LIMBAH PLASTIK
Authors: Riyadi Muhtarom , Sari Tri Wulan 
Published: January 2022

Beton menurut salah satu sumber yaitu SNI 2847:2013 dapat memiliki arti campuran semen Portland atau semen hidrolisis yang lain, agregat halus, agregat kasar, dan air, dengan atau tanpa bahan campuran tambahan (mixture). Tujuan dari penelitian ini adalah menganalisis sifat fisis dari agregat halus pasir dan limbah plastic seperti berat jenis SSD, berat jenis SSD, berat isi, Analisa ayak, kadar air, dan kadar lumpur. Metode yang dilakukan adalah menggunakan metode observasi dengan cara melakukan pengujian langsung di laboratorium sesuai Standar Nasional Indonesia (SNI). Hasil yang diperoleh menunjukkan bahwa pada penelitian agregat halus pasir dengan pengujian berat jenis SSD sebesar 2,55; penyerapan air sebesar 2,35%; berat isi / massa jenis lepas sebesar 1,33 Kg/liter; berat isi / massa jenis padat sebesar 1,56 Kg/liter; analisa ayak sebesar 2,90; kadar air sebesar 1,93%; dan kadar lumpur sebesar 3%. Nilai tersebut memenuhi standar spesifikasi pembuatan beton berdasarkan SNI 1969:2008. Sedangkan penelitian yang dilakukan terhadap agregat halus limbah plastik tidak memenuhi spesifikasi dan dapat dilihat pada pembahasan maupun lampiran.
Desain Autofill Smart Dispenser dengan Sistem Kendali Volume Air Otomatis dan Pengendali berbasis Android
Authors: Yadi Asri 
Published: January 2022

Air memiliki manfaat yang sangat besar bagi kehidupan manusia terutama untuk kebutuhan sehari-hari. Kebutuhan air minimal setiap orang perharinya adalah 121 liter baik itu untuk minum dan masak, cuci, mandi serta keperluan ibadah. Namun pemenuhan kebutuhan air ini sering mengalami kendala di kota-kota besar khususnya kantor dan pabrik. Agar persediaan air minum ini mudah dijangkau maka digunakan dispenser sebagai media untuk menampung air galon. Untuk tujuan tersebut diatas, maka pada penelitian ini dibahas tentang rancang bangun autofill smart dispenser dengan sistem otomatisasi dan berbasis android untuk memudahkan pengisian air pada gelas dan mencegah air minum tumpah dan cepat habis. Waktu pengisian dispenser berbasis android dimana rata-rata pengisian dispenser berbasis android adalah 17,96 detik dan dispenser otomatis sebesar 18,28 detik.
Classification of Toddler Nutritional Status Based on Antrophometric Index and Feature Discrimination using Support Vector Machine Hyperparameter Tuning
Authors: Much. afif masykur mughni  , Fahrudin Tresna Maulana , Kamisutara Made 
Published: January 2022

Nutritional status is the study of food and is related to health. Nutritional status is a benchmark to assess the health development of toddler. The nutritional status of toddler is assessed according to three index, such as body weight to age (BW / A), body height to age (BH / A), body weight to body height (BW / BH). The issue of nutrition is still a major factor in the growth and development of toddler in Indonesia. Public Health Center (Puskesmas) and Integrated Healthcare Center (Posyandu) as public health services work together to control the growth and development of toddler in Indonesia. To help control the growth and development of toddler, we proposed a research to classify the nutritional status of toddler based on anthropometric index. The nutritional status of toddler dataset was formed into a classification model using SVM Hyperparameter Tuning. SVM is a machine learning which the classification model used a hypothesis space in the form of linear functions in a high dimensional feature space. Adjustment of the hyperparameter was involved to reach a model that can optimally solve machine learning problems. We implemented feature selection using Fisher's Discriminant Ratio as a preprocessing stage, which the most important features were body weight (BB) and height (BH). The experimental results showed the classification model using SVM on training and testing data with a ratio of 70:30 reached accuracy of 84%, while SVM Hyperparameter Tuning with parameter of Cost = 100 parameters, Gamma = 0.01, Kernel = RBF reached accuracy of 97%. They represented a significant accuracy difference of 13%.
COLLABORATIVE GOVERNANCE SEBAGAI SOLUSI DALAM TATA KELOLA PEMBERDAYAAN NELAYAN
Authors: Fajri Hidayatul , Maani Karjuni Dt. , Wahyuni Nila , Malau Hasbullah 
Published: January 2022

Salah satu metode populer dalam model tata kelola saat ini adalah kolaboratif. Kolaborasi tersebut dinilai mampu menghimpun para pemangku kepentingan dalam suatu lembaga yang akan efektif dalam memecahkan masalah, salah satunya adalah masalah dalam pemberdayaan. Oleh karena itu, artikel ini bertujuan untuk membahas persepsi dari pemangku kepentingan terhadap bentuk pengelolaan pemberdayaan nelayan di Kabupaten Pesisir Selatan. Penelitian ini menggunakan pendekatan multi-kriteria sistematis untuk mengeksplorasi informasi tentang preferensi para pemangku kepentingan mengenai tujuan apa yang mereka anggap penting (apa), langkah-langkah dan strategi untuk mencapai tujuan mereka (bagaimana), dan opsi yang mereka tawarkan untuk meningkatkan tata kelola pemberdayaan. Hasil penelitian menunjukkan bahwa masing-masing stakeholder memiliki preferensi, dan langkah-langkah yang berbeda di dalam melihat tata kelola pemberdayaan, tetapi mereka memiliki opsi yang sama tentang bentuk tata kelola pemberdayaan yangÂ dianggap efektif yaitu pengelolaann pemberdayaan yang mampu melibatkan atau mengakomodasi banyak pihak. Sehingga, hasil penelitian ini menyarankan terbentuknya pengelolaan pemberdayaan nelayan yang berbentuk kolaboratif antara stakeholder yang ada di Kabupaten Pesisir Selatan. Dengan cara ini pemberdayaan nelayan dapat lebih baik dalam memfasilitasi kepentingan dan partisipasi dari stakeholder.