bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuropathy-associated Fars2 deficiency affects neuronal development and potentiates neuronal apoptosis by impairing mitochondrial function
Authors: Chen Xihui , Liu Fangfang , Li Bowen , Wang Yufeng , Yuan Lijuan , Yin Anan , Chen Qi , Hu Weihong , Yao Yan , Zhang Mengjie , Wu YuanMing , Chen Kun 
Published: July 2022

Abstract
Background
Neurodegenerative diseases encompass an extensive and heterogeneous group of nervous system disorders which are characterized by progressive degeneration and death of neurons. Many lines of evidence suggest the participation of mitochondria dysfunction in these diseases. Mitochondrial phenylalanyl-tRNA synthetase, encoded by FARS2, catalyzes the transfer of phenylalanine to its cognate tRNA for protein synthesis. As a member of mt-aaRSs genes, FARS2 missense homozygous mutation c.424G > T (p.D142Y) found in a Chinese consanguineous family first built the relationship between pure hereditary spastic paraplegia (HSP) and FARS2 gene. More FARS2 variations were subsequently found to cause heterogeneous group of neurologic disorders presenting three main phenotypic manifestations: infantile-onset epileptic mitochondrial encephalopathy, later-onset spastic paraplegia and juvenile onset refractory epilepsy. Studies showed that aminoacylation activity is frequently disrupt in cases with FARS2 mutations, indicating a loss-of-function mechanism. However, the underlying pathogenesis of neuropathy-associated Fars2 deficiency is still largely unknown.

Results
Early gestation lethality of global Fars2 knockout mice was observed prior to neurogenesis. The conditional Fars2 knockout-mouse model delayed lethality to late-gestation, resulting in a thinner cortex and an enlarged ventricle which is consist with the MRI results revealing cortical atrophy and reduced cerebral white matter volume in FARS2-deficient patients. Delayed development of neurite outgrowth followed by neuronal apoptosis was confirmed in Fars2-knockdown mouse primary cultured neurons. Zebrafish, in which fars2 was knocked down, exhibited aberrant motor neuron function including reduced locomotor capacity which well restored the spastic paraplegia phenotype of FARS2-deficient patients. Altered mitochondrial protein synthesis and reduced levels of oxidative phosphorylation complexes were detected in Fars2-deficient samples. And thus, reduced ATP, total NAD levels and mitochondrial membrane potential, together with increased ROS production, revealed mitochondrial dysfunction both in vitro and in vivo. Dctn3 is a potential downstream molecule in responds to Fars2 deficient in neurons, which may provide some evidence for the development of pathogenesis study and therapeutic schedule.

Conclusions
The Fars2 deficiency genetic models developed in this study cover the typical clinical manifestations in FARS2 patients, and help clarify how neuropathy-associated Fars2 deficiency, by damaging the mitochondrial respiratory chain and impairing mitochondrial function, affects neuronal development and potentiates neuronal cell apoptosis.
Ethanol is indispensable for virucidal hand antisepsis: memorandum from the alcohol-based hand rub (ABHR) Task Force, WHO Collaborating Centre on Patient Safety, and the Commission for Hospital Hygiene and Infection Prevention (KRINKO), Robert Koch Institute, Berlin, Germany
Authors: Kramer Axel , Arvand Mardjan , Christiansen Bärbel , Dancer Stephanie , Eggers Maren , Exner Martin , Müller Dieter , Mutters Nico T. , Schwebke Ingeborg , Pittet Didier 
Published: July 2022

Abstract
Background
The approval of ethanol by the Biocidal Products Regulation has been under evaluation since 2007. This follows concern over alcohol uptake from ethanol-based hand rubs (EBHR). If ethanol is classified as carcinogenic, mutagenic, or reprotoxic by the European Chemicals Agency (ECHA), then this would affect infection prevention and control practices.

Aim
A review was performed to prove that ethanol is toxicological uncritical and indispensable for hand antisepsis because of its unique activity against non-enveloped viruses and thus the resulting lack of alternatives. Therefore, the following main points are analyzed: The effectiveness of ethanol in hand hygiene, the evidence of ethanol at blood/tissue levels through hand hygiene in healthcare, and the evidence of toxicity of different blood/tissue ethanol levels and the non-comparability with alcoholic consumption and industrial exposure.

Results
EBHR are essential for preventing infections caused by non-enveloped viruses, especially in healthcare, nursing homes, food industry and other areas. Propanols are effective against enveloped viruses as opposed to non-enveloped viruses but there are no other alternatives for virucidal hand antisepsis. Long-term ingestion of ethanol in the form of alcoholic beverages can cause tumours. However, lifetime exposure to ethanol from occupational exposure < 500 ppm does not significantly contribute to the cancer risk. Mutagenic effects were observed only at doses within the toxic range in animal studies. While reprotoxicity is linked with abuse of alcoholic beverages, there is no epidemiological evidence for this from EBHR use in healthcare facilities or from products containing ethanol in non-healthcare settings.

Conclusion
The body of evidence shows EBHRs have strong efficacy in killing non-enveloped viruses, whereas 1-propanol and 2-propanol do not kill non-enveloped viruses, that pose significant risk of infection. Ethanol absorbed through the skin during hand hygiene is similar to consumption of beverages with hidden ethanol content (< 0.5% v/v), such as apple juice or kefir. There is no risk of carcinogenicity, mutagenicity or reprotoxicity from repeated use of EBHR. Hence, the WHO Task Force strongly recommend retaining ethanol as an essential constituent in hand rubs for healthcare.
Comparative analysis of intraoral scanners accuracy using 3D software: an in vivo study
Authors: Pellitteri Federica , Albertini Paolo , Vogrig Angelica , Spedicato Giorgio Alfredo , Siciliani Giuseppe , Lombardo Luca 
Published: July 2022

Abstract
Background
The purpose of the present in vivo study was to compare the accuracy, in terms of trueness, between full-arch digital impressions of different intraoral scanning systems, using as a reference the ideality of the conventional impression technique.

Methods
Polyvinyl siloxane (PVS) two-step technique impressions of 27 subjects were taken, and the stone casts were scanned using desktop scanners R500 3Shape. For each arch, in vivo scans were taken with intraoral scanners Carestream CS3600, CEREC Omnicam and Trios 3Shape. All the files were compared, superimposing them on the reference model to calculate the total 3D and 2D deviations. The efficiency of the digital and conventional workflows was evaluated by measuring the work time in minutes. Statistical analyses were performed using R software (R Core Team 2020) with a p-value < 0.05.

Results
The three intraoral scanners differed from the PVS impression by differences of the order of 100–200 µm, and there was a trend of greater imprecision in the molar area in both dental arches. In comparison with PVS technique, CEREC tended to reduce the size of the impression, Trios presented the trend of greater precision, and Carestream showed minor differences the transversal distance. The areas of greatest discrepancy both in excess and in defect with respect to the PVS impression were the molar areas and incisal margins. Trios 3Shape recorded the shortest times and therefore with a more performing speed.

Conclusion
The Trios 3Shape was found to be the most accurate single-tooth scanner, while the Carestream CS 3600 showed better inter-arch diameter performance compared to PVS impressions. The 3D and 2D analyses showed a trend of greater distortion of the impressions compared to the conventional one in the molar region.
Dual public health crises: the overlap of drug overdose and firearm injury in Indianapolis, Indiana, 2018–2020
Authors: Magee Lauren A. , Ray Bradley , Huynh Philip , O’Donnell Daniel , Ranney Megan L. 
Published: July 2022

Abstract
Background
Drug overdose and firearm injury are two of the United States (US) most unrelenting public health crises, both of which have been compounded by the COVID-19 pandemic. Programs and policies typically focus on each epidemic, alone, which may produce less efficient interventions if overlap does exist. The objective is to examine whether drug overdose correlates with and is associated with firearm injury at the census tract level while controlling for neighborhood characteristics.

Methods
An ecological study of census tracts in Indianapolis, Indiana from 2018 to 2020. Population rates per 100,000 and census tracts with the highest overlap of overdose and firearm injury were identified based on spatial clusters. Bivariate association between census tract characteristic and drug overdose and firearm violence rate within spatial clusters. Zero-inflated negative binominal regression was used to estimate if the drug overdose activity is associated with higher future firearm injury.

Results
In high overdose—high firearm injury census tracts, rates of firearm injury and drug overdose are two times higher compared to city wide rates. Indicators of structural disadvantage and structural racism are higher in high overdose—high firearm injury census tracts compared to city-wide averages. Drug overdoses are associated with higher rates of firearm injury in the following year (IRR: 1.004, 95% CI 1.001, 1.007, p < 0.05), adjusting for census tract characteristics and spatial dependence.

Conclusions
Drug overdose and firearm injury co-spatially concentrate within census tracts. Moreover, drug overdoses are associated with future firearm injury. Interventions to reduce firearm injuries and drug overdoses should be a co-response in high drug overdose—high firearm injury communities.
Gastrointestinal neuroendocrine tumor with discordant metastatic disease on 111In-pentetreotide SPECT/CT, 18F-DOPA PET/CT and 68Ga-HA-DOTATATE PET/CT
Authors: Resch Katrin , Hung Ryan , Abele Jonathan 
Published: July 2022

AbstractA 62-year-old man with resected, pathology-proven small bowel neuroendocrine tumor underwent 111In-pentetreotide SPECT/CT, 18F-DOPA PET/CT and 68Ga-HA-DOTATATE PET/CT to assess metastatic disease. The 111In-pentetreotide SPECT/CT scan showed no metastatic disease. Both 18F-DOPA and 68Ga-HA-DOTATATE PET/CT showed hepatic and peritoneal metastatic disease. However, the burden of 18F-DOPA-avid metastatic disease was far greater compared to the burden of 68Ga-HA-DOTATATE-avid metastatic disease.
The role of psychological autopsy in investigating a case of atypical suicide in schizophrenia: a case report with a brief review of literature
Authors: Crișan Roxana-Mihaela , Băcilă Ciprian Ionuț , Morar Silviu 
Published: July 2022

Abstract
Background
Self-harm typically is without lethal intent. Death can occur rarely, with suicide taking on an atypical form that raises the suspicion of hetero-aggression. Our study aimed to identify the link between self-harm and suicide intent and also to outline the positive diagnosis of an atypical suicide case which has raised the suspicion of hetero-aggression. For this purpose, the psychological autopsy method should be used regularly in suicide investigation because it not only allows a positive diagnosis of suicide but can also provide a detailed picture of mental degradation and associated suicide risk factors.

Case presentation
The case of a 26-year-old man from a rural area, found dead in the basement, at home, naked, barricaded inside, is described.

Methods
The on-site investigation and a complete forensic autopsy were performed. In addition, we apply the psychological autopsy method which gathered enough information to outline the positive diagnosis of suicide. We also made a brief literature review on the suicide risk factors and the behavioral changes that occurred during the COVID-19 pandemic in schizophrenic patients.

Results
The forensic autopsy revealed that he presented a complex craniofacial trauma as the cause of death (with scalp lacerations, frontal fracture, subarachnoid hemorrhage, and frontal cerebral contusions) associated with torso trauma (with self-inflicted stabbed wounds) with bruises and abrasions on the limbs. The injuries that caused death were self-inflicted and ensued repeatedly hitting his head against blunt objects. Using the psychological autopsy method, we found out that he presented multiple psychiatric hospitalizations for schizophrenia for almost 10 years, recently with reduced compliance to treatment. We also documented two previous suicide attempts and a gradual deterioration of his mental health.

Conclusions
We highlighted the role of the psychological autopsy (in addition to the judicial investigation and the forensic autopsy) for the diagnosis of committed suicide, for making a rigorous differential diagnosis between accident, hetero-aggression, and suicide, and also in pin-pointing the suicide risk factors.
The role of extent of resection on the prognosis of low-grade astrocytoma: a systematic review and meta-analysis
Authors: Chaulagain Dipak , Smolanka Volodymyr , Smolanka Andriy , Munakomi Sunil , Havryliv Taras 
Published: July 2022

Abstract
Purpose
To investigate the predictor factors of mortality describing the prognosis of primary surgical resection of low-grade astrocytoma.

Materials and methods
A systemic search was conducted from electronic databases (PubMed/Medline, Cochrane Library, and Google Scholar) from inception to November 14, 2021. All statistical analysis was conducted in Review Manager 5.4.1. Studies meeting inclusion criteria were selected. A random-effect model was used when heterogeneity was seen to pool the studies, and the result were reported in the hazards ratio (HR) and corresponding 95% confidence interval.

Result
Five cohort studies were selected for meta-analysis. There was statistically significant effect of total resection on increase mortality after surgery in low-grade astrocytoma patients (HR = 0.70 [0.52, 0.94]; p = 0.02; I2 = Not applicable). On the other hand, there was statistically nonsignificant effect of patient’s age (HR = 1.27 [0.95, 1.68]; p = 0.11; I2 = 83%), tumor size (HR = 1.13 [0.94, 1.35]; p = 0.19; I2 = 73%), and increasing KPS (HR = 0.59 [0.20, 1.77]; p = 0.35; I2 = 86%) on prognosis of low-grade astrocytoma after surgery.

Conclusion
The results of meta-analysis showed significant relationship of extent of resection and mortality, while factors such age, KPS score, and tumor size were nonsignificant to determine mortality in patient diagnosed with low-grade astrocytoma. The gross total resection surgery should be preferred over subtotal resection since the incidence of malignant formation is low in gross total resection.
Review of regulation techniques of asphalt pavement high temperature for climate change adaptation
Authors: Gong Zhenlong , Zhang Letao , Wu Jiaxi , Xiu Zhao , Wang Linbing , Miao Yinghao 
Published: July 2022

AbstractAsphalt pavement is vulnerable to the temperature rising and extremely high-temperature weather caused by climate change. The regulation techniques of asphalt pavement high temperature have become a growing concern to adapt to climate change. This paper reviewed the state of the art on regulating asphalt pavement high temperature. Firstly, the influencing factors and potential regulation paths of asphalt pavement temperature were summarized. The regulation techniques were categorized into two categories. One is to regulate the heat transfer process, including enhancing reflection, increasing thermal resistance, and evaporation cooling. The other is to regulate through heat collection and transfer or conversion, including embedded heat exchange system, phase change asphalt pavement, and thermoelectric system. Then, the regulation techniques in the literature were reviewed one by one in terms of cooling effects and pavement performance. The issues that still need to be improved were also discussed. Finally, the regulation techniques were compared from the perspectives of theoretical cooling effects, construction convenience, and required maintenance. It can provide reference for understanding the development status of asphalt pavement high temperature regulation techniques and technique selection in practice.
Pore system characterization in diagenetically complex Mississippian-aged carbonate reservoirs (Kansas, USA)
Authors: Bode-Omoleye Ibukun , Grammer G. Michael , Zhang Chi , Molnar Adam 
Published: July 2022

Advanced petrophysical methods and detailed statistical analysis help to quantitatively and qualitatively characterize pore structure variations in carbonate rocks. Using a core from the Mississippian limestone play in south-central Kansas, we have investigated geologic constraints on reservoir properties using observations of the variability in pore architecture and measured petrophysical properties, i.e., porosity, permeability, and nuclear magnetic resonance (NMR) response. The sample set includes facies that retain their original depositional texture and rocks that have been subject to dolomitization, silicification, and dissolution to varying extents. The dominant macropore types include intercrystalline, moldic, and dissolution-enhanced vuggy porosity, whereas the dominant microporosity types are micritic and dolomitic intercrystalline micropores and nano-intercrystalline within silicified samples. The porosity-permeability relationships and [Formula: see text] modal distribution of the carbonates that we investigated correlate with dominant pore types depending on the extent and type of diagenetic modification. The fractal nature of pore systems in dolograinstones is not apparent in partially dolomitized samples, and pore complexity significantly decreases with increasing dolomitization. [Formula: see text] and bound fluid volume estimates indicate that independent of geologic origin, small and intricate pore systems with microporosity are likely to host higher amounts of capillary-bound fluids. This relationship holds, especially among samples with nano-intercrystalline porosity. Correlation coefficients from predicting NMR-derived parameters with porosity improved from 0.1 to 0.78 by including pore architecture data and pore types in the multiple linear regression model. The results and observations presented here improve the current understanding and predictability of petrophysical parameters in carbonate rocks with complex pore systems.
Pore structure analysis of tight reservoirs in He-8 Member of Upper Paleozoic in the southwestern Ordos Basin, China
Authors: Wei Qinlian , Mei Jiahao , Tian Jingchun , Chen Xi , Xiao Ling 
Published: July 2022

Quantitative characterization of the pore structure in tight reservoir can provide basic parameters for the evaluation of the reservoir quality. By using the cast thin-section petrography, scanning electron microscopy, mercury injection capillary pressure, and constant-rate mercury injection, we have evaluated the distribution characteristics of pore throat and its contribution to permeability. In addition, we have established the relationship between different pore-throat structure parameters and reservoir physical, which provides theoretical support for reservoir evaluation of the He-8 Member in the study area. The results indicate that the pore structure exerts a great control on the quality of the reservoir. Large pore throats (>0.15 μm) are the main contributors to the high seepage capacity of the reservoir. Throat radius, throat distribution, and pore-throat ratio are the key factors controlling the quality of tight reservoir. The He-8 Member of the Upper Paleozoic in the study area is dominated by tight reservoirs, accompanied by a small number of conventional reservoirs.
Updated analysis from the ATEZO-BRAIN trial: Atezolizumab plus carboplatin and pemetrexed in patients with advanced nonsquamous non–small cell lung cancer with untreated brain metastases.
Authors: Nadal Ernest , Rodriguez-Abreu Delvys , Massuti Bartomeu , Juan-Vidal Oscar , Huidobro Vence Gerardo , Lopez Rafael , de Castro Carpeño Javier , Estival Anna , Campelo Rosario Garcia , Sullivan Ivana , Felip Enriqueta , Blasco Ana , Guirado Maria , Vilarino Noelia , Simo Marta , Sanahuja Marta , Hernandez Anna , Navarro Valentin , Bruna Jordi 
Published: July 2022

9010 Background: Atezolizumab plus chemotherapy was safe and yielded promising clinical outcome as frontline therapy for patients (pts) with advanced NSCLC with untreated brain metastases (BM) in the ATEZO-BRAIN study (NCT03526900). Methods: A multicenter single-arm phase II trial with a Bayesian design for evaluating the safety and efficacy of atezolizumab plus carboplatin with pemetrexed every 3 weeks for 4-6 cycles, followed by maintenance with pemetrexed plus atezolizumab in pts with stage IV non-squamous NSCLC without EGFR or ALK genetic alterations and untreated BM. Pts not presented neurologic symptoms at baseline; but anticonvulsants and dexamethasone (DXM) ≤ 4mg qd were allowed. Co-primary endpoints were safety and investigator-assessed progression-free survival (PFS) at 12 weeks according to RANO-BM and RECIST v1.1 for brain and systemic disease, respectively. Here we present the final data and an exploratory analysis based on PD-L1 expression and corticosteroid treatment at baseline. Results: Out of 40 pts included in the study, 22 (55%) were receiving DXM at baseline and 20 (50%) had positive expression of PD-L1. Sixteen (40%) pts had confirmed intracranial response based on RANO-BM (12 PR, 4 CR) and 19 (47.5%) pts achieved systemic response (all PR). Only 4 pts had discordant responses between the body and the brain. No differences were observed in the overall systemic and intracranial response rate according to the PD-L1 expression or the use of corticosteroids at baseline. As of December 31, 2021 (median follow-up, 20 months), the updated median (95% CI) systemic PFS was 8.9 (6.7 to 13.8) and intracranial PFS was 6.9 (4.7 to 11.9). Median (95% CI) OS was 13.6 (9.72 to not reached) and estimated 2-year OS rate (95% CI) was 30.5% (18.4 to 50.4). Median (95%CI) OS was longer for PD-L1 positive pts (16.2; 10.3 to not reached) compared to PD-L1 negative pts (10.7; 7.6 to not reached) but differences were not statistically significant due to the limited statistical power (HR = 0.99; 95% CI 0.35 to 2.12). No significant differences in OS were observed between pts receiving or not baseline DXM treatment. Treatment was well tolerated and no grade 5 toxicities were observed. Conclusions: In this updated analysis, treatment with atezolizumab plus carboplatin and pemetrexed yields a promising 2-year OS rate and intracranial response rate in patients with untreated BM from NSCLC, regardless of treatment with corticosteroids at baseline and PD-L1 expression. Clinical trial information: NCT03526900.