bluuebunny/crossref_metadata_2025_split huggingface dataset:
Initiation of hydroxychloroquine therapy during pregnancy can cause adverse effects and alter pregnancy outcomes: A case of acute generalised exanthematous pustulosis induced by hydroxychloroquine in a patient with systemic lupus erythematosus
Authors: Tsurane Kotoi , Kaneko Kayoko , Yoshida Kazue , Tanaka Ryo , Sago Haruhiko , Murashima Atsuko 
Published: November 2022

ABSTRACTHydroxychloroquine (HCQ) is effective for treating a number of autoimmune diseases, including systemic lupus erythematosus. HCQ is generally safe and may be prescribed to pregnant women. Although current guidelines recommend initiating HCQ when considering pregnancy, the drug can cause adverse effects such as acute generalised exanthematous pustulosis (AGEP), which should be carefully evaluated. A 30-year-old pregnant woman with systemic lupus erythematosus at 16 + 5 gestational weeks was referred to National Center for Child Health and Development for persistent proteinuria and alopecia. Tacrolimus was initiated, and the dose of prednisone was increased. At 20 + 3 weeks of gestation, HCQ was administered to allow for a dose reduction of prednisolone. Proteinuria gradually improved as the pregnancy course stabilised. At 27 + 1 weeks of gestation, generalised pustular exanthema developed, presumably due to HCQ. Based on the clinical course and the analysis of the skin lesions, she was diagnosed to have either AGEP or generalised pustular psoriasis. Despite discontinuing HCQ, the skin lesions worsened dramatically, and infliximab therapy was required. After one course of infliximab treatment, exanthema gradually subsided. The final diagnosis was AGEP, based on the clinical course and pathological findings. At 30 weeks, pyothorax developed because of the pyogenic skin lesion and the compromised immune system, and long-term antibiotic therapy was required until 32 + 4 weeks, after which she underwent caesarean section. Although introducing HCQ is occasionally necessary during pregnancy, it is preferable to initiate HCQ in the preconception period and not after pregnancy because of the possible adverse effect, which can alter perinatal prognosis. Rheumatologists should consider the potential risks of HCQ.
Monolithic integrated micro-supercapacitors with ultra-high systemic volumetric performance and areal output voltage
Authors: Wang Sen , Li Linmei , Zheng Shuanghao , Das Pratteek , Shi Xiaoyu , Ma Jiaxin , Liu Yu , Zhu Yuanyuan , Lu Yao , Wu Zhong-Shuai , Cheng Hui-Ming 
Published: November 2022

ABSTRACT
Monolithic integrated micro-supercapacitors (MIMSCs) with high systemic performance and cell-number density are important for miniaturized electronics to empower the Internet of Things. However, fabrication of customizable MIMSCs in an extremely small space remains a huge challenge considering key factors such as materials selection, electrolyte confinement, microfabrication and device-performance uniformity. Here, we develop a universal and large-throughput microfabrication strategy to address all these issues by combining multistep lithographic patterning, spray printing of MXene microelectrodes and controllable 3D printing of gel electrolytes. We achieve the monolithic integration of electrochemically isolated micro-supercapacitors in close proximity by leveraging high-resolution micropatterning techniques for microelectrode deposition and 3D printing for precise electrolyte deposition. Notably, the MIMSCs obtained demonstrate a high areal-number density of 28 cells cm−2 (340 cells on 3.5 × 3.5 cm2), a record areal output voltage of 75.6 V cm−2, an acceptable systemic volumetric energy density of 9.8 mWh cm−3 and an unprecedentedly high capacitance retention of 92% after 4000 cycles at an extremely high output voltage of 162 V. This work paves the way for monolithic integrated and microscopic energy-storage assemblies for powering future microelectronics.
Effectiveness of a Third Dose of COVID-19 mRNA Vaccine During the Omicron BA.1- and BA.2-Predominant Periods in Japan: The VENUS Study
Authors: Mimura Wataru , Ishiguro Chieko , Maeda Megumi , Murata Fumiko , Fukuda Haruhisa 
Published: November 2022

Abstract

Background
Vaccine effectiveness against the severe acute respiratory syndrome coronavirus 2 Omicron BA.2 sublineage in Japan is unknown. We assessed the effectiveness of a third dose of COVID-19 mRNA vaccine compared with that of 2 doses.

Methods
We performed a population-based cohort study using a municipality database located in the Chubu region of Japan during the Omicron BA.1- and BA.2-predominant periods (January 1–March 31, 2022 and April 1–27, 2022, respectively). We included residents aged ≥16 years who received a second vaccine dose at ≥14 days before the start of each period, regardless of the third dose. We compared the data at 14 days after the second and third dose and at 2-week intervals from 14 days to 10 weeks after the third dose using a Cox regression model. Vaccine effectiveness was defined as (1 – hazard ratio) × 100 (%).

Results
In total, 295 705 and 288 184 individuals were included in the BA.1- and BA.2-predominant periods, respectively. The effectiveness of a third dose against infection was 62.4% and 48.1% in the BA.1- and BA.2-predominant periods, respectively. Vaccine effectiveness at 2–3 weeks and ≥10 weeks after the third dose decreased from 63.6% (95% confidence interval [CI], 56.4–69.5%) to 52.9% (95% CI, 41.1–62.3%) and from 54.5% (95% CI, 3.0–78.7%) to 40.1% (95% CI, 15.1–57.7%) in the BA.1- and BA.2-predominant periods, respectively.

Conclusions
A third dose was moderately effective against BA.1 and BA.2 sublineages, but its effectiveness decreased by approximately 10% age points from 2–3 weeks to ≥10 weeks after the third vaccination.
Spatial distribution and computational modeling for mapping of tuberculosis in Pakistan
Authors: Khaliq Aasia , Ashraf Uzma , Chaudhry Muhammad N , Shahid Saher , Sajid Muhammad A , Javed Maryam 
Published: November 2022

Abstract

Background
Tuberculosis (TB) like many other infectious diseases has a strong relationship with climatic parameters.

Methods
The present study has been carried out on the newly diagnosed sputum smear-positive pulmonary TB cases reported to National TB Control Program across Pakistan from 2007 to 2020. In this study, spatial and temporal distribution of the disease was observed through detailed district wise mapping and clustered regions were also identified. Potential risk factors associated with this disease depending upon population and climatic variables, i.e. temperature and precipitation were also identified.

Results
Nationwide, the incidence rate of TB was observed to be rising from 7.03% to 11.91% in the years 2007–2018, which then started to decline. However, a declining trend was observed after 2018–2020. The most populous provinces, Punjab and Sindh, have reported maximum number of cases and showed a temporal association as the climatic temperature of these two provinces is higher with comparison to other provinces. Machine learning algorithms Maxent, Support Vector Machine (SVM), Environmental Distance (ED) and Climate Space Model (CSM) predict high risk of the disease with14.02%, 24.75%, 34.81% and 43.89% area, respectively.

Conclusion
SVM has a higher significant probability of prediction in the diseased area with a 1.86 partial receiver-operating characteristics (ROC) value as compared with other models.
The circadian variation of sleep and alertness of postmenopausal women
Authors: Pérez-Medina-Carballo Rafael , Kosmadopoulos Anastasi , Boudreau Philippe , Robert Manon , Walker Claire-Dominique , Boivin Diane B 
Published: November 2022

Abstract

Study Objectives
Several factors may contribute to the high prevalence of sleep disturbances occurring in postmenopausal women. However, the contribution of the circadian timing system to their sleep disturbances remains unclear. In the present study, we aim to understand the impact of circadian factors on changes of sleep and alertness occurring after menopause.

Methods
Eight healthy postmenopausal women and 12 healthy young women in their mid-follicular phase participated in an ultradian sleep–wake cycle procedure (USW). This protocol consisted of alternating 60-min wake periods and nap opportunities for ≥ 48 h in controlled laboratory conditions. Core body temperature (CBT), salivary melatonin, self-reported alertness, and polysomnographically recorded sleep were measured across this procedure.

Results
In both groups, all measures displayed a circadian variation throughout the USW procedure. Compared to young women, postmenopausal women presented lower CBT values, more stage N1 and N2 sleep, and number of arousals. They also showed a reduced amplitude of the circadian variation of melatonin, total sleep time (TST), sleep onset latency (SOL), stage N3 sleep, and alertness levels. Postmenopausal women fell asleep faster and slept more during the biological day and presented higher alertness levels during the biological night than young women.

Conclusion
These results support the hypothesis of a weakened circadian signal promoting sleep and wakefulness in older women. Aging processes including hormonal changes may be main contributors to the increased sleep–wake disturbances after menopause.
Development of an expression-tunable multiple protein synthesis system in cell-free reactions using T7-promoter-variant series
Authors: Senda Naoko , Enomoto Toshihiko , Kihara Kenta , Yamashiro Naoki , Takagi Naosato , Kiga Daisuke , Nishida Hirokazu 
Published: November 2022

Abstract
New materials with a low environmental load are expected to be generated through synthetic biology. To widely utilize this technology, it is important to create cells with designed biological functions and to control the expression of multiple enzymes. In this study, we constructed a cell-free evaluation system for multiple protein expression, in which synthesis is controlled by T7 promoter variants. The expression of a single protein using the T7 promoter variants showed the expected variety in expression levels, as previously reported. We then examined the expression levels of multiple proteins that are simultaneously produced in a single well to determine whether they can be predicted from the promoter activity values, which were defined from the isolated protein expression levels. When the sum of messenger ribonucleic acid (mRNA) species is small, the experimental protein expression levels can be predicted from the promoter activities (graphical abstract (a)) due to low competition for ribosomes. In other words, by using combinations of T7 promoter variants, we successfully developed a cell-free multiple protein synthesis system with tunable expression. In the presence of large amounts of mRNA, competition for ribosomes becomes an issue (graphical abstract (b)). Accordingly, the translation level of each protein cannot be directly predicted from the promoter activities and is biased by the strength of the ribosome binding site (RBS); a weaker RBS is more affected by competition. Our study provides information regarding the regulated expression of multiple enzymes in synthetic biology.
The Extensor Digiti Quinty (EDQ) Opponensplasty—Revisited
Authors: Dengler Jana , Roth Bettlach Carrie L. , Riggi Margot , Moore Amy M. 
Published: November 2022

Introduction:
The extensor digiti quinty (EDQ) opponensplasty, although previously described, is not widely used by hand surgeons. This tendon transfer was used selectively in cases of combined median-ulnar nerve palsies at our center over the last 4 years, with the primary goal to preserve the extensor indices proprius (EIP) for pinch plasty. We present our technique and the series of 8 patients that underwent EDQ opponensplasty here.

Materials and Methods:
A retrospective chart review identified patients with combined median-ulnar nerve palsies that underwent EDQ opponensplasty between 2015 and 2019 at our institution by a single surgeon. Primary outcome measures collected were pinch, grip, disabilities of the arm, shoulder, and hand (DASH) scores, thumb position, palmar abduction angle, and thumb span. Secondary outcomes included donor site morbidity and complications.

Results:
Eight patients (9 hands) ages 21 to 53 underwent EDQ opponensplasty, with an average follow-up of 8 months. Postoperative pinch and grip on the affected side improved from preoperative values. All 9 transfers were functional with the thumb positioned in the palmar plane of the hand. Palmar abduction ranged from 45 to 70 degrees. Motor reeducation was achieved without any difficulty in all patients. No patients had evidence of donor site morbidity.

Discussion:
The EDQ opponensplasty is satisfactory in achieving thumb opposition in the setting of combined median-ulnar neuropathy, without evidence of donor site morbidity. The EDQ is expendable, has adequate length, an optimal line of pull, minimal donor site morbidity, and leaves the extensor indices proprius available for pinch plasty.
Starfysh reveals heterogeneous spatial dynamics in the breast tumor microenvironment
Authors: He Siyu , Jin Yinuo , Nazaret Achille , Shi Lingting , Chen Xueer , Rampersaud Sham , Dhillon Bahawar S. , Valdez Izabella , Friend Lauren E , Fan Joy Linyue , Park Cameron Y , Mintz Rachel , Lao Yeh-Hsing , Carrera David , Fang Kaylee W , Mehdi Kaleem , Rohde Madeline , McFaline-Figueroa José L. , Blei David , Leong Kam W. , Rudensky Alexander Y , Plitas George , Azizi Elham 
Published: November 2022

AbstractSpatially-resolved gene expression profiling provides valuable insight into tissue organization and cell-cell crosstalk; however, spatial transcriptomics (ST) lacks single-cell resolution. Current ST analysis methods require single-cell RNA sequencing data as a reference for a rigorous interpretation of cell states and do not utilize associated histology images. Significant sample variation further complicates the integration of ST datasets, which is essential for identifying commonalities across tissues or altered cellular wiring in disease. Here, we present Starfysh, the first comprehensive computational toolbox for joint modeling of ST and histology data, dissection of refined cell states, and systematic integration of multiple ST datasets from complex tissues. Starfysh uses an auxiliary deep generative model that incorporates archetypal analysis and any known cell state markers to avoid the need for a single-cell-resolution reference in characterizing known or novel tissue-specific cell states. Additionally, Starfysh improves the characterization of spatial dynamics in complex tissues by leveraging histology images and enables the comparison of niches as spatial “hubs” across tissues. Integrative analysis of primary estrogen receptor-positive (ER+) breast cancer, triple-negative breast cancer (TNBC), and metaplastic breast cancer (MBC) tumors using Starfysh led to the identification of heterogeneous patient- and disease-specific hubs as well as a shared stromal hub with varying spatial orientation. Our results show the ability to delineate the spatial co-evolution of tumor and immune cell states and their crosstalk underlying intratumoral heterogeneity in TNBC and revealed metabolic reprogramming shaping immunosuppressive hubs in aggressive MBC. Starfysh is publicly available (https://github.com/azizilab/starfysh).
Transgenerational Effects of Early-Life Stress on Anxiety in Zebrafish (Danio rerio)
Authors: Fontana Barbara D. , Alnassar Nancy , Parker Matthew O. 
Published: November 2022

AbstractEarly-life adversity impacts on anxiety-related behaviors in adulthood. The effects of such adversity not only affects the animal itself, but can be passed on transgenerationally. Pervasive effects of experimentally-induced early-life stress (ELS) have been documented in adult zebrafish but it is not clear if this can be passed on via the germline. Here, we investigated the effects of ELS across three generations, by analyzing the responses of adult animals exposed to ELS in two different anxiety-related tasks, as well as in social behavior, memory, and cognition. Animals exposed to ELS (at 7 days-post-fertilization) showed a marked attenuation of specific anxiety-related behaviors (F0) when adults, and these alterations were maintained across two subsequent generations (F1and F2). These findings suggest that zebrafish may be a useful model organism to study the transgenerational effects of ELS, and how this pertains to (for example) neuropsychiatric disorders. In addition, our data may naturally provoke questions regarding consideration of the environment of laboratory-housed zebrafish at early developmental stages. In particular, more work may be necessary to determine how different environmental stressors could affect data variability across laboratories.Graphical AbstractSummary of the ELS effects in zebrafish anxiety-like behavior across multiple generations.
The application of robotics and artificial intelligence in embroidery: challenges and benefits
Authors: Chen Ling , Su Zhi , He Xiaotong , Chen Xiang , Dong Lin 
Published: November 2022

Purpose
Embroidery as a textile embellishment technique plays an important role in people's daily life. Esthetic embroidery artworks possess cultural values. With the development of robotics and artificial intelligence (AI), these technologies have been studied and applied in the embroidery process. This study aims to survey how these technologies facilitate embroidery from different aspects.

Design/methodology/approach
This paper surveys how the technologies of robotics and AI are applied in the embroidery field. The applications are mainly reviewed from three aspects: computerized robotic embroidery systems has been widely used for the mass production of embroidered textiles, the advanced technological systems and techniques have greatly facilitated the development of smart textiles and the artificial intelligence plays an important role in the inheritance, innovation and protection of traditional handicraft artwork of embroidery.

Findings
The programmable robotic embroidery machines have greatly improved the production efficiency of embroidered textiles and promoted the development of electronic textiles. The AI, mainly the deep learning technology, brings significant benefits to esthetic embroidery creation. Technology-based embroidery has become a hot research topic in the field of textiles.

Originality/value
This paper summarizes the application of robotics and AI technologies in the field of embroidery, which provides readers a comprehensive and systematic understanding about the research progress of modern technology-oriented embroidery. This helps readers gain inspiration from the technology perspectives.
A meta-analysis of online travel booking services adoption: the moderating effects of national or regional economic development level and culture differences
Authors: Li Tiantian , Zhu Zhangxiang 
Published: November 2022

PurposeThis study aims to provide a systematic review and meta-analysis to explore the strength of the path relationship in the proposed model for online travel booking service adoption. It also explores the moderating effect of national or regional economic development levels and cultural differences.Design/methodology/approachA meta-analysis was conducted on the factors correlated with users' intention to adopt online travel booking services (OTBS) and the moderating effects of economic development levels and culture based on 42 empirical studies.FindingsPerceived usefulness, perceived ease of use, attitude and perceived behavioral control have a significant positive impact on adoption intention. By contrast, subjective norms have a significantly negative impact on adoption intention. Furthermore, the economic development level of a country or region significantly moderates the relationships between perceived usefulness and perceived behavior control, attitude and perceived behavior control, and subjective norms and adoption intention. At the same time, national or regional cultural differences significantly moderate the relationships between attitudes and adoption intention, perceived usefulness and perceived behavior control, and subjective norms and adoption intention.Originality/valueThis study was conducted to obtain a unified conclusion regarding the research field of online travel booking service adoption. Its content was original. The conclusion provides theoretical references for follow-up research and the development of targeted marketing programs for online travel-booking service providers.
Determinants of entrepreneurial performance of rural indigenous women entrepreneurs in Sarawak, Malaysia
Authors: Yap Ching Seng , Keling William , Ho Poh Ling 
Published: November 2022

Purpose
This study aims to explore the influence of internal and external factors on the entrepreneurial performance of rural indigenous women entrepreneurs (RIWEs) in Sarawak.

Design/methodology/approach
The study uses a qualitative approach in data collection and analysis. Data are collected by means of a semistructured interview from 19 RIWEs at rural areas in Sarawak. Data are then analyzed using thematic analysis in NVivo software.

Findings
The study finds that RIWEs’ decision to start their business is mainly motivated by pull factors such as ego to succeed, ability to be self-employed and financially independent and to contribute to household income. The important personality traits of RIWEs to succeed in the entrepreneurial venture include self-belief, perseverance, commitment and risk-taking. Functional competencies and relationship competencies are cited as the most important core competencies to succeed. In terms of external factors, social networks, financial resources and support, business training and workshops and government’s preferential treatment policies are found to be important to RIWE’s entrepreneurial success.

Originality/value
This study is one of the few that explores the determinants of successful RIWEs in an emerging economy.