bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental and numerical simulation study of the effect for the anode positions on the discharge characteristics of 300 W class low power Hall thrusters
Authors: CHEN 陈 Xinwei 新伟 , GAO 高 Jun 俊 , YANG 杨 Sanxiang 三祥 , GENG 耿 Hai 海 , GUO 郭 Ning 宁 , GU 顾 Zuo 左 , YANG 杨 Juntai 俊泰 , ZHANG 张 Hong 宏 
Published: June 2022

Abstract
Low-power Hall thruster (LHT) generally has poor discharge efficiency characteristics due to the large surface-to-volume ratio. Aiming to further refine and improve the performance of 300 W class LHT in terms of thrust and efficiency, and to obtain the most optimal operating point, the experimental study of the discharge characteristics for three different anode positions was conducted under the operation of various discharge voltages (100–400 V) and anode mass flow rates (0.65 mg·s−1 and 0.95 mg·s−1). The experimental results indicated that the thruster has the most excellent performance in terms of thrust and efficiency etc at a channel length of 27 mm for identical operating conditions. In addition, particle in cell simulations, employed to reveal the underlying physical mechanisms, show that the ionization and acceleration zone is pushed downwards towards the channel exit as the anode moves towards the exit. At the identical operating point, when the channel length is reduced from 32 to 27 mm, the ionization and acceleration zone moves towards the exit, and the parameters such as thrust and efficiency increase due to the high ionization rate, ion number density, and axial electric field. When the channel length is further moved to 24 mm, the parameters in terms of thrust (F) and efficiency (

η

a

) are reduced as a result of the decreasing ionization efficiency (

η

m

) and the larger plume divergence angle (

α

). In this paper, the results indicated that an optimum anode position (

Δ
L
=
27

mm) exists for the optimum performance.
The curvature effect in Gaussian random fields
Authors: Levada Alexandre L M 
Published: June 2022

Abstract
Random field models are mathematical structures used in the study of stochastic complex systems. In this paper, we compute the shape operator of Gaussian random field manifolds using the first and second fundamental forms (Fisher information matrices). Using Markov chain Monte Carlo techniques, we simulate the dynamics of these random fields and compute the Gaussian, mean and principal curvatures of the parametric space, analyzing how these quantities change along dynamics exhibiting phase transitions. During the simulations, we have observed an unexpected phenomenon that we called the curvature effect, which indicates that a highly asymmetric geometric deformation happens in the underlying parametric space when there are significant increase/decrease in the system’s entropy. When the system undergoes a phase transition from randomness to clustered behavior the curvature is smaller than that observed in the reverse phase transition. This asymmetric pattern relates to the emergence of hysteresis phenomenon, leading to an intrinsic arrow of time along the random field dynamics.
Reading Mozambique’s mega-project developmentalism through the workplace: evidence from Chinese
and Brazilian investments
Authors: Cezne Eric , Wethal Ulrikke 
Published: June 2022

ABSTRACT
Propelled by a commodities boom and expanding South–South investment, mega-projects have reshaped the politics of labour in many African settings. Reflecting on such dynamics, this article critically engages with questions of employment, skills development, and contestation re-configuring capital–labour encounters in the ‘Chinese’ and ‘Brazilian’ workplace in Mozambique. We analyse two mega-projects: the Maputo Ring Road, implemented by the China Road and Bridge Corporation, and the Moatize Coal Project, led by the Brazilian mining company, Vale SA. Engaging with the complex realities at project ground level, the article unpacks how workplace regimes and outcomes reflect an intricate, multi-scalar array of spatial encounters, sector-specific characteristics, and national political economies. For both cases, this is associated with common promises of development and prosperity for Mozambique. While such promises take on different ideational guises, we show that the Chinese and Brazilian workplaces expose, nonetheless, overlapping patterns of inequality, contention, and hostility, reinforced by broader vulnerabilities and imbalances in global production networks and the Mozambican political economy. By providing a ground-level reading of the multi-scalar forces at play in the workplace, this article sheds light on the relationship between emerging South–South global encounters, national political realities, and labour geographies in African contexts shaped by mega-projects.
In vitro colonic fermentation characteristics of barley-koji differ from those of barley
Authors: Nagata Ryuji , Morioka Minami , Fukuma Naoki , Hayashi Kei , Iwami Akihiko , Han Kyu-Ho , Fukushima Michihiro 
Published: June 2022

ABSTRACT
Barley-koji is prepared by inoculating barley, a beneficial prebiotic source, with the fungi Aspergillus luchuensis mut. kawachii. In this study, the prebiotic effects of barley-koji on human colonic microbiota were evaluated in vitro compared with barley, using pig feces. The enzyme-resistant fraction of the following sample groups each was added to respective fermenters: cellulose, barley (Commander and β104), and barley-koji (Commander-koji and β104-koji). Short-chain fatty acid and ammonia-nitrogen production increased and decreased, respectively, in barley-koji and barley groups. Furthermore, the propionate concentration increased in the barley group, showing a positive correlation with the abundance of the genus Dialister. In the barley-koji group, however, acetate and n-butyrate concentrations increased during the early stages of incubation, and the relative abundance of the genus Megasphaera was higher than those of the other genera. Therefore, this study demonstrated that barley-koji might possess beneficial physiological properties for colonic fermentation, which differ from those of barley.
Mapping developmental paths of monkey primordial germ-like cells differentiation from pluripotent stem cells by single cell ribonucleic acid sequencing analysis
Authors: Zhang Puyao , Xue Sengren , Guo Rongrong , Liu Jian , Bai Bing , Li Dexuan , Hyraht Ahjol , Sun Nianqin , Shao Honglian , Fan Yong , Ji Weizhi , Yang Shihua , Yu Yang , Tan Tao 
Published: June 2022

Abstract
The induction of primordial germ-like cells (PGCLCs) from pluripotent stem cells (PSCs) provides a powerful system to study the cellular and molecular mechanisms underlying germline specification, which are difficult to study in vivo. The studies reveal the existence of a species-specific mechanism underlying PGCLCs between humans and mice, highlighting the necessity to study regulatory networks in more species, especially in primates. Harnessing the power of single-cell RNA sequencing (scRNA-seq) analysis, the detailed trajectory of human PGCLCs specification in vitro has been achieved. However, the study of nonhuman primates is still needed. Here, we applied an embryoid body (EB) differentiation system to induce PGCLCs specification from cynomolgus monkey male and female PSCs, and then performed high throughput scRNA-seq analysis of approximately 40 000 PSCs and cells within EBs. We found that EBs provided a niche for PGCLCs differentiation by secreting growth factors critical for PGCLC specification, such as bone morphogenetic protein 2 (BMP2), BMP4, and Wnt Family Member 3. Moreover, the developmental trajectory of PGCLCs was reconstituted, and gene expression dynamics were revealed. Our study outlines the roadmap of PGCLC specification from PSCs and provides insights that will improve the differentiation efficiency of PGCLCs from PSCs.
‘Villagers in the City’: Resilience in migrant youth amidst urbanisation
Authors: Lu Shuang 
Published: June 2022

Abstract
Rapid urbanisation necessitates more research attention to the challenges and resources among families moving from rural to urban areas. Using a multisystemic resilience framework, this qualitative study explores how resilience is embodied in the intrapersonal, interpersonal and institutional levels of the daily lives of migrant children in China, an exemplar of contemporary fast-urbanising societies. I conducted semi-structured in-depth interviews with ten migrant children who live in Beijing, along with their parents, teachers and social service providers. The findings suggest that rural-to-urban migration is both an empowering and a depriving experience. On the intrapersonal level, migration increases children’s independence, personal growth, future aspirations, as well as academic stress. On the interpersonal level, migrant children receive substantial support from parents, siblings, extended family and peers; however, they are at risk of insufficient parental supervision and loss of meaningful relationships. On the institutional level, migration provides children with a better environment, better school experiences, greater material resources and social organisation services, but migrant children remain vulnerable to educational discrimination, economic deprivation and poor neighbourhood environments in the city.
The AHNAK induces increased IL-6 production in CD4+ T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss
Authors: Li Liman , Liu Yuan , Feng Ting , Zhou Wenjie , Wang Yanyun , Li Hong 
Published: June 2022

Abstract
Disorganized maternal–fetal immune tolerance contributes to the occurrence of unexplained recurrent pregnancy loss (RPL). AHNAK is a scaffolding protein participating in the regulation of Ca2+ entry into T cells and the pathophysiology of diverse diseases. We performed differential gene expression analysis in decidual immune cells (DICs) isolated from three patients with RPL and from three healthy controls via RNA-sequencing (RNA-seq), which revealed 407 differentially expressed genes (DEGs). Among these DEGs, we underscored the clinical significance of elevated AHNAK mRNA and protein levels in DICs, peripheral blood mononuclear cells (PBMCs), and decidua of the patients with RPL, suggesting its potential use as a biomarker for the diagnosis of RPL. Especially, the ratios of decidual and blood AHNAK+CD4+ T cells in the CD4+ T cell population were significantly increased in patients with RPL, and the loss of AHNAK was further shown to inhibit interleukin (IL)-6 secretion in the CD4+ Jurkat cell line. Similar patterns were also observed in the clinical decidual and blood specimens. We uncovered that the AHNAK+CD4+ T cells could secrete more IL-6 than that the corresponding AHNAK-CD4+ T cells. Moreover, the frequencies of decidual and blood IL-6+CD4+ T cells in the CD4+ T-cell population were also increased in patients with RPL and showed significant positive correlations with the frequencies of AHNAK+CD4+ T cells. Our findings suggest that the elevated AHNAK expressed by CD4+ T cells may be involved in the immune dysregulation of RPL by increasing IL-6 production, illustrating its potential as a novel intervention target for RPL.
Arrhythmic risk prediction in arrhythmogenic right ventricular cardiomyopathy: external validation of the arrhythmogenic right ventricular cardiomyopathy risk calculator
Authors: Jordà Paloma , Bosman Laurens P , Gasperetti Alessio , Mazzanti Andrea , Gourraud Jean Baptiste , Davies Brianna , Frederiksen Tanja Charlotte , Weidmann Zoraida Moreno , Di Marco Andrea , Roberts Jason D , MacIntyre Ciorsti , Seifer Colette , Delinière Antoine , Alqarawi Wael , Kukavica Deni , Minois Damien , Trancuccio Alessandro , Arnaud Marine , Targetti Mattia , Martino Annamaria , Oliviero Giada , Pipilas Daniel C , Carbucicchio Corrado , Compagnucci Paolo , Dello Russo Antonio , Olivotto Iacopo , Calò Leonardo , Lubitz Steven A , Cutler Michael J , Chevalier Philippe , Arbelo Elena , Priori Silvia Giuliana , Healey Jeffrey S , Calkins Hugh , Casella Michela , Jensen Henrik Kjærulf , Tondo Claudio , Tadros Rafik , James Cynthia A , Krahn Andrew D , Cadrin-Tourigny Julia 
Published: June 2022

Abstract

Aims
Arrhythmogenic right ventricular cardiomyopathy (ARVC) causes ventricular arrhythmias (VAs) and sudden cardiac death (SCD). In 2019, a risk prediction model that estimates the 5-year risk of incident VAs in ARVC was developed (ARVCrisk.com). This study aimed to externally validate this prediction model in a large international multicentre cohort and to compare its performance with the risk factor approach recommended for implantable cardioverter-defibrillator (ICD) use by published guidelines and expert consensus.

Methods and results
In a retrospective cohort of 429 individuals from 29 centres in North America and Europe, 103 (24%) experienced sustained VA during a median follow-up of 5.02 (2.05–7.90) years following diagnosis of ARVC. External validation yielded good discrimination [C-index of 0.70 (95% confidence interval-CI 0.65–0.75)] and calibration slope of 1.01 (95% CI 0.99–1.03). Compared with the three published consensus-based decision algorithms for ICD use in ARVC (Heart Rhythm Society consensus on arrhythmogenic cardiomyopathy, International Task Force consensus statement on the treatment of ARVC, and American Heart Association guidelines for VA and SCD), the risk calculator performed better with a superior net clinical benefit below risk threshold of 35%.

Conclusion
Using a large independent cohort of patients, this study shows that the ARVC risk model provides good prognostic information and outperforms other published decision algorithms for ICD use. These findings support the use of the model to facilitate shared decision making regarding ICD implantation in the primary prevention of SCD in ARVC.
Association between hypotension during 24 h ambulatory blood pressure monitoring and reflex syncope: the SynABPM 1 study
Authors: Rivasi Giulia , Groppelli Antonella , Brignole Michele , Soranna Davide , Zambon Antonella , Bilo Grzegorz , Pengo Martino , Sharad Bashaaer , Hamrefors Viktor , Rafanelli Martina , Testa Giuseppe Dario , Rice Ciara , Kenny Rose Anne , Sutton Richard , Ungar Andrea , Fedorowski Artur , Parati Gianfranco 
Published: June 2022

Abstract

Aims
Diagnostic criteria for ambulatory blood pressure monitoring (ABPM) in patients with suspected reflex syncope are lacking. The study hypothesis was that patients with reflex syncope have a higher prevalence of systolic blood pressure (SBP) drops on ABPM.

Methods and results
ABPM data from reflex syncope patients and controls, matched by average 24 h SBP, age, sex, and hypertension were compared. Patients with constitutional hypotension, orthostatic hypotension, and predominant cardioinhibition during carotid sinus massage or prolonged electrocardiogram monitoring or competing causes of syncope were excluded. Daytime and nighttime SBP drops (&lt;110, 100, 90, 80 mmHg) were assessed. Findings were validated in an independent sample. In the derivation sample, daytime SBP drops were significantly more common in 158 syncope patients than 329 controls. One or more daytime drops &lt;90 mmHg achieved 91% specificity and 32% sensitivity [odds ratio (OR) 4.6, P &lt; 0.001]. Two or more daytime drops &lt;100 mmHg achieved 84% specificity and 40% sensitivity (OR 3.5, P = 0.001). Results were confirmed in the validation sample of 164 syncope patients and 164 controls: one or more daytime SBP drops &lt;90 mmHg achieved 94% specificity and 29% sensitivity (OR 6.2, P &lt; 0.001), while two or more daytime SBP drops &lt;100 mmHg achieved 83% specificity and 35% sensitivity (OR 2.6, P &lt; 0.001)

Conclusion
SBP drops during ABPM are more common in reflex syncope patients than in controls. Cut-off values that may be applied in clinical practice are defined. This study expands the current indications for ABPM to patients with reflex syncope.
Divergent gut microbiota in two closely related house mouse subspecies under common garden conditions
Authors: Bendová Barbora , Mikula Ondřej , Vošlajerová Bímová Barbora , Čížková Dagmar , Daniszová Kristina , Ďureje Ľudovít , Hiadlovská Zuzana , Macholán Miloš , Martin Jean-Francois , Piálek Jaroslav , Schmiedová Lucie , Kreisinger Jakub 
Published: June 2022

AbstractThe gastrointestinal microbiota (GM) is considered an important component of the vertebrate holobiont. GM–host interactions influence the fitness of holobionts and are, therefore, an integral part of evolution. The house mouse is a prominent model for GM–host interactions, and evidence suggests a role for GM in mouse speciation. However, previous studies based on short 16S rRNA GM profiles of wild house mouse subspecies failed to detect GM divergence, which is a prerequisite for the inclusion of GM in Dobzhansky–Muller incompatibilities. Here, we used standard 16S rRNA GM profiling in two mouse subspecies, Mus musculus musculus and M. m. domesticus, including the intestinal mucosa and content of three gut sections (ileum, caecum, and colon). We reduced environmental variability by sampling GM in the offspring of wild mice bred under seminatural conditions. Although the breeding conditions allowed a contact between the subspecies, we found a clear differentiation of GM between them, in all three gut sections. Differentiation was mainly driven by several Helicobacters and two H. ganmani variants showed a signal of codivergence with their hosts. Helicobacters represent promising candidates for studying GM–host coadaptations and the fitness effects of their interactions.
A chromosome-level genome assembly and intestinal transcriptome of Trypoxylus dichotomus (Coleoptera: Scarabaeidae) to understand its lignocellulose digestion ability
Authors: Wang Qingyun , Liu Liwei , Zhang Sujiong , Wu Hong , Huang Junhao 
Published: June 2022

Abstract
Lignocellulose, as the key structural component of plant biomass, is a recalcitrant structure, difficult to degrade. The traditional management of plant waste, including landfill and incineration, usually causes serious environmental pollution and health problems. Interestingly, the xylophagous beetle, Trypoxylus dichotomus, can decompose lignocellulosic biomass. However, the genomics around the digestion mechanism of this beetle remain to be elucidated. Here, we assembled the genome of T. dichotomus, showing that the draft genome size of T. dichotomus is 636.27 Mb, with 95.37% scaffolds anchored onto 10 chromosomes. Phylogenetic results indicated that a divergent evolution between the ancestors of T. dichotomus and the closely related scarabaeid species Onthophagus taurus occurred in the early Cretaceous (120 million years ago). Through gene family evolution analysis, we found 67 rapidly evolving gene families, within which there were 2 digestive gene families (encoding Trypsin and Enoyl-(Acyl carrier protein) reductase) that have experienced significant expansion, indicating that they may contribute to the high degradation efficiency of lignocellulose in T. dichotomus. Additionally, events of chromosome breakage and rearrangement were observed by synteny analysis during the evolution of T. dichotomus due to chromosomes 6 and 8 of T. dichotomus being intersected with chromosomes 2 and 10 of Tribolium castaneum, respectively. Furthermore, the comparative transcriptome analyses of larval guts showed that the digestion-related genes were more commonly expressed in the midgut or mushroom residue group than the hindgut or sawdust group. This study reports the well-assembled and annotated genome of T. dichotomus, providing genomic and transcriptomic bases for further understanding the functional and evolutionary mechanisms of lignocellulose digestion in T. dichotomus.
Moho depths for Antarctica Region by the inversion of ground-based gravity data
Authors: Borghi Alessandra 
Published: June 2022

SUMMARYIn the last years the scientific literature has been enriched with new models of the Moho depth in the Antarctica Continent derived by the seismic reflection technique and refraction profiles, receiver functions and seismic surface waves, but also by gravimetric observations over the continent. In particular, the gravity satellite missions of the last two decades have provided data in this remote region of the Earth and have allowed the investigation of the crust properties. Meanwhile, other important contributions in this direction has been given by the fourth International Polar Year (IPY, 2007–2008) which started seismographic and geodetic networks of unprecedented duration and scale, including airborne gravimetry over largely unexplored Antarctic frontiers. In this study, a new model for the Antarctica Moho depths is proposed. This new estimation is based on no satellite gravity measures, thanks to the availability of the gravity database ANTGG2015, that collects gravity data from ground-base, airborne and shipborne campaigns. In this new estimate of the Moho depths the contribution of the gravity measures has been maximized reducing any correction of the gravity measures and avoiding constraints of the solution to seismological observations and to geological evidence. With this approach a pure gravimetric solution has been determined. The model obtained is pretty in agreement with other Moho models and thanks to the use of independent data it can be exploited also for cross-validating different Moho depths solutions.
Imprints of subducted Palaeo-Tethys oceanic lithosphere on upper-mantle discontinuities and the formation of the Emeishan large igneous province
Authors: He Chuansong 
Published: June 2022

SUMMARYGenerally, the upwelling of a mantle plume is suggested to have led to the formation of the Emeishan large igneous province (ELIP). However, this idea has been challenged by recent geological and seismological studies. In this investigation, three profiles of velocity perturbations crossing the ELIP are drawn following previous tomographic studies, and common conversion point (CCP) stacking of receiver functions in the ELIP is carried out. A slab-like high-velocity structure is revealed, which may be associated with the lithosphere of the Palaeo-Tethys Ocean (LPTO) subducting toward the northeast. CCP stacking of receiver functions shows that the LPTO generated imprints on the 410- and 660-km discontinuities. A comprehensive analysis suggests that the LPTO may have induced large-scale mantle upwelling, which contributed to the formation of the ELIP.
O-050 Vulnerability of human preimplantation embryos to SARS-CoV-2 infection
Authors: Viotti M 
Published: June 2022

Abstract
Presence of SARS-CoV-2, the virus responsible for COVID-19, has been reported in numerous organs and tissues of infected patients, including the reproductive system. The effects of COVID-19 on human reproduction remain poorly understood. While cases of intrauterine transmission between expectant mother and fetus have been documented, the impact of SARS-CoV-2 infection on early embryogenesis and establishment of a pregnancy are not known. This prompted us to ask if SARS-CoV-2 can infect embryos, since such an event could impact embryo viability and affect a subsequent pregnancy. We used a three-pronged approach to investigate this possibility: 1) Using RNA-seq and immunofluorescence, we learned that ACE2 and TMPRSS2, two factors required on target cells for SARS-CoV-2 entry, are co-expressed in cells of the trophectoderm in blastocyst-stage preimplantation embryos; 2) Using fluorescent reporter virions pseudotyped with Spike (S) glycoprotein from SARS-CoV-2, we observed robust infection of trophectoderm cells, and this permissiveness could be attenuated with blocking antibodies targeting S or ACE2; and 3) Exposing human blastocysts to live, fully infectious SARS-CoV-2, we detected cases of infection that compromised embryo health. Therefore, we identify a new human target tissue for SARS-CoV-2 with potential medical implications for reproductive health during the COVID-19 pandemic and its aftermath.