bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute gastroenteritis outbreaks caused by human astrovirus, 1978-2021: a systematic review
Authors: Fu Jiamei , Yu Fan , Li Hanning , Shen Lingyu , Tian Yi , Gao Zhiyong 
Published: September 2022

Abstract
Background: Human astrovirus (HAstV) is one of the main pathogens that causes sporadic cases of acute gastroenteritis, sometimes leading to outbreaks. This study aimed to understand the epidemiological and etiological characteristics of HAstV outbreaks worldwide.
Methods: The literature on HAstV outbreaks published before January 2022 was retrieved from China National Knowledge Infrastructure, WanFang, WeiPu, PubMed, and Web of Science databases and the date, region, population, setting, transmission modes, clinical symptoms, and etiological characteristics of the outbreaks were collected and analyzed.
Results: Twenty-one articles on 21 HAstV outbreaks reported from November 1978 to November 2017 were included. Up to three outbreaks were reported per year, with most having been reported in autumn (8/20, 40.00%). Outbreaks were primarily distributed in the northern hemisphere, with China reporting the most, and mainly occurred in childcare centers/kindergartens (6/21, 28.57%), hospitals (5/21, 23.81%), and schools (4/21, 19.05%). Virus genotypes were identified during 15 outbreaks, and HAstV-1 was the predominant (8/15, 53.33%). Abdominal pain and diarrhea were the most common symptoms.
Conclusions: Although HAstV outbreaks are not frequently reported, it is necessary to consider HAstV of norovirus-negative gastroenteritis outbreaks. In addition, local Centers for Disease Control and Prevention should have the capacity to handle HAstV outbreaks and identify the pathogen.
PROSPERO 2022: CRD42022334636
Systematic benchmarking of bulk and single-cell RNA-seq statistical methods to assess differential expression of circular RNAs
Authors: Buratin Alessia , Bortoluzzi Stefania , Gaffo Enrico 
Published: September 2022

Abstract
Background

Circular RNAs (circRNAs) are transcripts with covalently joined 3' and 5' ends. They play critical regulatory roles, are involved in disease and cancer mechanisms, and are promising biomarkers. CircRNA differential abundance in high-throughput sequencing transcriptomics (RNA-seq) studies is commonly analysed using differential expression assessment methods (DEMs) devised for bulk RNA-seq. However, circRNA expression data from RNA-seq have a high proportion of small and zero counts that might hamper commonly used DEMs. Importantly, DEMs' performance has never been evaluated on circRNA data.

Results

We compared 38 DEM pipelines on circRNA expression data, exploring DEMs' parameter configurations conceived for small counts. We also evaluated single-cell RNA-seq (scRNA-seq) and metagenomics DEMs designed to handle sparse data. In general, the DEMs performed poorly on data sets of typical size. Widely used bulk RNA-seq DEMs, such as DESeq2, edgeR, and Limma-Voom, gave scarce results, unreliable predictions, or even contravened the expected behaviour with some parameter configurations. Limma-Voom achieved the most consistent performance throughout different benchmark data and, as well as SAMseq, reasonably balanced false discovery and recall rates. A few scRNA-seq DEMs obtained results comparable to the best performing bulk RNA-seq tools. Almost all DEMs' performance improved when increasing the number of replicates.

Conclusions

CircRNA expression data have particular characteristics distinguished from traditional bulk RNA-seq, requiring careful study design, choice of DEM, and DEM configuration. This study can guide scientists in selecting the appropriate tools for circRNA comparative RNA-seq analyses.
Effect of Breathing Pattern and Posture on Abdominal Muscle Activation and Intra-abdominal Pressure
Authors: Kawabata Masashi , Shima Norihiro 
Published: September 2022

Abstract
We aimed to examine the association between several breathing patterns and postures on abdominal muscle activation and intra-abdominal pressure (IAP). Fourteen healthy men performed four active breathing tasks: quiet nasal breathing (Q-Bre), nasal diaphragmatic breathing (Dia-Bre), completely forced expiration (Forced-Expi), and exertional nasal inhalation with abdominal muscles in isometric contraction (Exertion-Inspi) in the elbow-toe and supine posture. Breathing volume, IAP, and transverse abdominis-internal oblique muscle (TrA-IO) and external oblique muscle (EO) activity were recorded. Abdominal muscle activity and IAP were significantly associated with breathing pattern and postures during the expiratory phase. In the inspiratory phase, TrA-IO activity were significantly associated with breathing pattern and EO activity with posture. TrA-IO activity significantly increased in Forced-Expi in the supine posture (47.6% of the maximum voluntary contraction) and Exertion-Inspi in the elbow-toe posture (35.7%), while no differences were found for Dia-Bre or Q-Bre (<20%). EO activity increased in the elbow-toe posture (22.5–30.6%) compared with that in the supine posture (<5%) for all breathing tasks. IAP values were low for all tasks (<15%) except Forced-Expi (24.9%). Breathing pattern, including posture, is a crucial element for determining abdominal muscle activity in exercise instruction.
The validity and reliability of a Direct Observation of Procedural Skills assessment tool: assessing knee joint mobilization skills of physical therapy students both on-site and off-site
Authors: Hou Yu-Jen , Liu Wen-Yu , Hsieh Ming-Ju , Lien Hen-Yu , Wang Tzyy-Jiuan , Cheng Chih-Hsiu , Yau Sze-Yuen , Lin Yang-Hua 
Published: September 2022

Abstract

Background: There is a need to validate the reliability and validity of Direct Observation of Procedural Skills (DOPS) of knee joint mobilization for providing immediate feedback on the procedure. Additionally, it is crucial to examine whether the tools developed for on-site use can be applied off-site to prepare for future practice. The purpose of this study is to describe the implementation and clinimetric properties of a DOPS for knee joint mobilization evaluation (DOPSknee) on-site and off-site and to determine the pre-clinical competencies of physical therapy (PT) students at school.
Methods: After the DOPSknee had been developed, 42 pre-clinical students from a single PT program were videotaped performing knee joint mobilization. Their performance was assessed in the standard manner by two clinical instructors using the DOPSknee. Assessments were conducted on-site and then off-site using the recorded videos after a time gap ranging from 1.5 months to 4.5 months.
Results: The DOPSknee demonstrated a good level of on-site inter-rater reliability (intraclass correlation coefficient [ICC] = 0.78). It had a poor level of off-site inter-rater reliability (ICC = 0.37) and a poor to good level of intra-rater reliability between on-site and off-site evaluations (evaluator 1: ICC = 0.42; evaluator 2: ICC = 0.88). However, the DOPSknee was shown to have good reliability average measures between the on-site and off-site evaluations (average on-site vs. average off-site: ICC = 0.84). Average total DOPSknee scores were significantly correlated with the average global rating scores on-site and off-site (r= 0.47 and 0.75) and in cross-over conditions (r = 0.54 and 0.71). The cut-off score for the DOPSkneewas determined to be 14 points, and the construct validity analysis of both on-site and off-site evaluations from the average DOPSknee total scores demonstrated that the average global rating scores of the students who passed were significantly higher (p< 0.05).
Conclusions: The clinimetric properties of the DOPSknee protocol revealed generally good results for on-site validity and reliability in assessing student performance and a need to be interpreted and applied with care off-site. The average measures may help improve the results from the two evaluators.
Multicenter Cohort Study on Duration of Antiarrhythmic Medication for Supraventricular Tachycardia in Infants
Authors: Mecklin Minna , Linnanmäki Anniina , Hiippala Anita , Leino Topias , Arola Anita , Leskinen Markku , Ruotsalainen Hanna , Happonen Juha-Matti , Poutanen Tuija 
Published: September 2022

Abstract
Purpose
Antiarrhythmic medication (AM) is commonly used to prevent supraventricular tachycardia (SVT) recurrence in infants. Our aim was to determine whether a shorter duration of AM is sufficient to prevent atrioventricular reentrant tachycardia (AVRT) recurrence and evaluate risk factors for recurrence of SVT after discontinued AM.
Methods
This multicenter cohort study included all infants diagnosed with SVT in the five university hospitals in Finland between 2005 and 2017. Those diagnosed between 2005 and 2012 received AM for 12 months (Group 1), and those diagnosed between 2013 and 2017 received AM for six months (Group 2).
Results
A total of 278 infants presented with AVRT (Group 1, n = 181; Group 2, n = 97), and the median AM duration was 366 days (interquartile range [IQR] 345–407) and 212 days (IQR 181–310), respectively. Propranolol was the most frequently used first-line AM (92% and 95%). Recurrence-free survival rates were over 88% until 12 months after AM prophylaxis in both groups, without any statistically significant difference between them. Independent risk factors for recurrence of SVT after discontinuation of AM were need of combination AM (HR 2.2, 95% CI 1.14–4.20), Wolff-Parkinson White (WPW) syndrome (HR 2.4, 95% CI 1.25–4.59) and age older than one month at admission (HR 2.2, 95% CI 1.12–4.48).
Conclusions
Shortening AM duration in infants from 12 to six months does not lead to more frequent SVT recurrence. The risk factors for recurrence of SVT were WPW syndrome, need of combination AM and age older than one months.
m6A regulator-mediated methylation modification patterns and immune microenvironment infiltration characterization in osteoarthritis
Authors: Hu Shidong , Shen Chen , Yao Xudong , Zou Yulong , Wang Ting , Sun Xianding , Nie Mao 
Published: September 2022

Abstract
Osteoarthritis (OA) is a common disease in orthopedics. RNA N6-methyladenosine (m6A) exerts an essential effect in a variety of biological processes in the eukaryotes. In this study, we determined the effect of m6A regulators in the OA along with performing the subtype classification. Differential analysis of OA and normal samples in the database of Gene Expression Omnibus (GEO) identified 9 significantly differentially expressed m6A regulators. These regulators were monitored by a random forest algorithm so as to evaluate the risk of developing OA disease. On the basis of these 9 moderators, a nomogram was established. The results of decision curve analysis (DCA) suggested that the patients could benefit from a nomogram model. The OA sample was classified as 2 m6A models through a consensus clustering algorithm in accordance with these 9 regulators. These 2 m6A patterns were then assessed with principal component analysis (PCA). We also determined the m6A scores for the 2 m6A patterns and their correlation with immune infiltration. The results indicated that type A had a higher m6A score than type B. Thus, we suggest that the m6A pattern may provide a new approach for diagnose and provide novel ideas for molecular targeted therapy of OA.
Association of pars defect type with clinical outcome after smiley face rod methods for terminal-stage spondylolysis
Authors: Okuwaki Shun , Tatsumura Masaki , Gamada Hisanori , Asai Reo , Nagashima Katsuya , Takeuchi Yosuke , Funayama Toru , Yamazaki Masashi 
Published: September 2022

Abstract
Background
The smiley face rod method is an effective treatment for symptomatic terminal-stage spondylolysis. However, the risk factors for treatment failure are unknown. We investigated the association of pars defect type with the treatment outcomes of this method.
Methods
We retrospectively examined data from 34 patients (18.0 ± 6.7 years) with terminal-stage spondylolysis who underwent surgery using the smiley face rod method. The mean follow-up period was 44.9 ± 21.4 months. The patients were divided into 2 groups: pars defect without bone atrophy or sclerosis (group A; 18 patients), and with bone atrophy and sclerosis (group B; 16 patients). The visual analog scale (VAS) score for back pain, bone union rate, and time to return to preinjury level athletics were evaluated and compared between the groups. Fisher’s exact test and a paired t test were used when comparing the groups. VAS score between the groups were analyzed using a 2-factor repeated-measures analysis of variance.
Results
Within groups there were significant differences over time for VAS score (p < 0.001). No significant differences were observed between groups for VAS scores. Group A was significantly higher bone union rate per pars at 6 months (65.7% and 37.5%, p = 0.028) and 24 months after surgery (97.1% and 75.0%, p = 0.011). All patients returned to their respective sports, and no significant differences were observed with time to return to preinjury level athletics between the groups (p = 0.055).
Conclusion
These results suggested that the defect of pars sclerosis is associated with bone nonunion, but has little effect on postoperative symptoms.
Investigation of theoretical hydroelectric potential with GIS-based model in Buyuk Menderes Basin, Turkey
Authors: KOÇ Cengiz , BAYAZIT Yıldırım , ÖZGÜL Selami Yurdan 
Published: September 2022

Abstract

Hydroelectric power plants, which are a renewable energy source, have a significant role to play in the fight against climate change and global warming. For this reason, it is of great importance for a country to use its domestic resources in the most efficient and effective way. Using a GIS-based model method, locations having theoretical hydroelectric energy potential were identified in the Buyuk Menderes basin, one of Turkey's largest basins. According to the installed power potential on the examined basin, 33 sites below 10 MW, 57 sites between 10 and 50 MW, 4 sites between 50 and 100 MW, and 1 site over 100 MW were found as a consequence of the study. At the same time, the reliability of the results was debated after they were compared to the installed power of two important hydroelectric power facilities in the basin. The analysis found that the installed power of Adıgüzel and Kemer HEPPs in the relevant site, which had 62 MW and 48 MW installed power, respectively, was 80 MW and 69 MW. The calculations assume that the turbine is 100% efficient. However, when considering a turbine efficiency of 70%-80%, the findings are close to the true values. While the theoretical hydroelectric energy potential in the whole basin is 886.55 MW at 70% turbine efficiency, the installed power in the basin is currently 302.02 MW. According to these results, only 34.07% of the total hydro potential in the basin is used today.
Trends in the Relationship Between Suicide Behaviors and Obesity Among Adolescents in the United States from 1999-2019
Authors: Iwatate Eriko , Atem Folefac D. , Jones Eric C. , Hughes Jennifer L. , Yokoo Takeshi , Messiah Sarah E. 
Published: September 2022

Abstract
Background
Previous studies indicate obesity is a risk factor of suicide behaviors among adolescents. Whether this association has remained consistent during the ongoing obesity epidemic remains unknown.
Method
The time trends of the obesity-suicide association were examined using the 1999–2019 biannual Youth Risk Behavior Survey data (n = 161,606). Prevalence odds ratio of suicide behaviors among obese adolescents (vs. non-obese) for each survey year and time trends using National Cancer Institute joinpoint regression analysis were calculated.
Results
For each year post-baseline, there was a significant increase of prevalence odds ratio of 1.4 (1.2–1.6)-1.6(1.3-2.0) for ideation, 1.3(1.1–1.7)-1.7(1.4-2.0) for plan, and 1.3(1.0-1.7)-1.9(1.5–2.4) for an attempt, except for the 2013 survey for the attempt (1.19 [0.9–1.6]). Significant positive trends were found during 1999–2019 for ideation and plan, with biannual %-changes of + 0.92% and + 1.22%, respectively.
Conclusion
Obese adolescents have consistently higher odds of engaging in suicide behaviors than their non-obese peers since the beginning of the national obesity epidemic, and that this association grew stronger as the obesity epidemic continued
Melamine sponge skeleton loaded organic conductors for mechanical sensors with high sensitivity and high resolution
Authors: Yufeng Wu , Jianbo Wu , Yan Lin , Junchen Liu , Xiaolong Pan , Xian He , Ke Bi , Ming Lei 
Published: September 2022

Abstract

In recent years, due to the development of flexible electronics, flexible sensors have been widely concerned and applied in intelligent robots, brain-computer interfaces and wearable electronic devices. We propose a low-cost and high-efficiency sensor component preparation method. The sensor Tetrathiafulvalene-Tetracyanoquinodimethane/ melamine sponge (TTMS) takes a melamine sponge as a flexible substrate and metallizes the sponge with the organic conducting molecule Tetrathiafulvalene-Tetracyanoquinodimethane (TTF-TCNQ) to construct a conductive pathway with chemical stability. We use a physical load approach to ensure the advantages of low cost and efficient manufacturing. TTMS has good mechanical stability and can withstand 8000 compressions. 1000 cycles of cyclic voltammetry scanning proved that it also had good electrical stability. TTMS can distinguish pressure changes of 100 Pa and respond quickly to pressure application and release. These TTMS can be assembled to form an array of sensors that can distinguish the position and intensity of pressure. Therefore, the excellent performance of the sensor is expected to promote the commercial application of the piezoresistive sensor.
Optimization of a breath analysis methodology to potentially diagnose transplanted kidney rejection
Authors: De Vietro Nicoletta , Aresta Antonella Maria , Picciariello Arcangelo , Altomare Donato Francesco , Digilio Alessia , Palmisani Jolanda , De Gennaro Gianluigi , Zambonin Carlo 
Published: September 2022

Abstract
Chronic kidney disease may result in end stage renal disorder and increased mortality rate. Up to date, kidney transplantation represents the only definitive treatment to restore normal life expectancy. Nevertheless, there is a high risk of organ rejection in the short-medium term after surgery. This paper proposes a new ReCIVA®-GC-MS technology to potentially predict the rejection of the transplanted kidney during the first year after surgery by analyzing human breath. Twenty VOCs, recognized by literature as targets or as representative of the major classes of molecules essential to the identification of chronic kidney disease affected patients and/or healthy subjects, were selected and employed for this methodology optimization. The breath profile of healthy subjects was considered as target in case of restored kidney function after transplantation. Calibration curves, linearity concentration range, limit of detection and quantification of selected molecules were estimated as well as the intra-day and inter-day reproducibility of the method. To test the applicability of the GC-MS developed methodology, the breath of healthy subjects and chronic kidney disease affected patients was ReCIVA® sampled, and then analyzed. Sixty-seven molecules were identified, and between these, thirteen of twenty selected compounds were quantified, confirming the robustness of the optimized protocol.
Distribution of Toxic Metals concentration among High-Rate Food crops in Iran: A probabilistic Risk Assessment
Authors: Ghalhari Mohammad Rezvani , Fahiminia Mohammad , Alimohammadi Mahmood , Rahimi Nayereh Rezaei , Azhdarpoor Abooalfazl , Koochakzadeh Zeynab , Fouladi-Fard Reza 
Published: September 2022

Abstract
Ingestion has been as an important pathway which can increase the intake of various chemical pollutants such as toxic heavy metals (HMs) into body; vegetables and high-rate food crops can contain high HMs concentration based on their irrigation water source. However, vegetable consumption can reduce the incidence of various diseases and disabilities. This study aimed to measure the concentration of HMs in the crops, and evaluation the carcinogenic and non-carcinogenic risks. In the present study, 30 crop samples were collected from popular Qom market, then the HMs concentrations were determined by inductively coupled plasma-mass spectrometry (ICP-MS). The results showed that the mean concentration of Pb, Cu, Zn, As, and Cd were 21.1 ± 7.14, 156.44 ± 129.02, 288.46 ± 114.59, 31.51 ± 16.95, and 26.96 ± 23.01 µg/kg in crops samples, respectively. Also results shown that the rank order of HMs concentration of HI was As > Zn > Cd > Cu > Pb and for ELCR was Cd > As > Pb among children and adults. The 95th percentile of calculated hazard index (HI) of Pb, Cu, Zn, As, and Cd among children and adults were 2.84 and 1.88, respectively (target HI > 1.0). Therefore, continuous consumption of crops can cause adverse health effects in children and adults. Also, the 95th percentile of calculated ELCR of Pb, As, and Cd among children and adults were 5.93E-06 and 1.84E-05, respectively, because of the ELCR value in this group is in the WHO target, so, before either taking action or designing low priority, further investigation was required.
Development and testing of a 2D offshore oil spill modeling tool (OSMT) supported by an effective calibration method
Authors: Yang Zhaoyang , Chen Zhi , Lee Kenneth 
Published: September 2022

Abstract
Spill trajectory prediction (STP) is essential to decision-making support for oil pollution preparedness and response. An Oil Spill Modeling Tool (OSMT) has been developed in this study to predict the transport and fate of oil spills from surface releases. Particularly, the Kullback-Leibler (KL) divergence method is adopted as a performance metric for the first time to formulate a calibration framework for STP from the Lagrangian transport model (LTM). By finding the candidate with minimal KL divergences from modeling scenarios using designed parameter combinations, the prediction discrepancy between simulated trajectories of the LTM and oil slicks detected from satellite images are reduced. The developed approach has first been evaluated through a numerical comparison analysis between OSMT and Operational Oil Modeling Environment (GNOME) model to examine model validity in a hypothetical case. Subsequently, a real case study is conducted to examine the applicability and effectiveness of the KL divergence-based oil spill trajectory modeling method. The study results indicate that OSMT can provide reliable spill trajectory simulations, and the KL divergence-based calibration method is effective in calibrating the oil spill LTM.