bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanical thrombectomy in minor stroke due to isolated M2 occlusion: a multicenter retrospective matched analysis
Authors: Alexandre Andrea M , Colò Francesca , Brunetti Valerio , Valente Iacopo , Frisullo Giovanni , Pedicelli Alessandro , Scarcia Luca , Rollo Claudia , Falcou Anne , Milonia Luca , Andrighetti Marco , Piano Mariangela , Macera Antonio , Commodaro Christian , Ruggiero Maria , Da Ros Valerio , Bellini Luigi , Lazzarotti Guido A , Cosottini Mirco , Caragliano Armando A , Vinci Sergio L , Gabrieli Joseph D , Causin Francesco , Panni Pietro , Roveri Luisa , Limbucci Nicola , Arba Francesco , Pileggi Marco , Bianco Giovanni , Romano Daniele G , Diana Francesco , Semeraro Vittorio , Burdi Nicola , Ganimede Maria P , Lozupone Emilio , Fasano Antonio , Lafe Elvis , Cavallini Anna , Russo Riccardo , Bergui Mauro , Calabresi Paolo , Della Marca Giacomo , Broccolini Aldobrando 
Published: October 2022

BackgroundThe purpose of this study was to evaluate the effectiveness of mechanical thrombectomy (MT) in patients with isolated M2 occlusion and minor symptoms and identify possible baseline predictors of clinical outcome.MethodsThe databases of 16 high-volume stroke centers were retrospectively screened for consecutive patients with isolated M2 occlusion and a baseline National Institutes of Health Stroke Scale (NIHSS) score ≤5 who received either early MT (eMT) or best medical management (BMM) with the possibility of rescue MT (rMT) on early neurological worsening. Because our patients were not randomized, we used propensity score matching (PSM) to estimate the treatment effect of eMT compared with the BMM/rMT. The primary clinical outcome measure was a 90-day modified Rankin Scale score of 0–1.Results388 patients were initially selected and, after PSM, 100 pairs of patients receiving eMT or BMM/rMT were available for analysis. We found no significant differences in clinical outcome and in safety measures between patients receiving eMT or BMM/rMT. Similar results were also observed after comparison between eMT and rMT. Concerning baseline predicting factors of outcome, the involvement of the M2 inferior branch was associated with a favorable outcome.ConclusionOur multicenter retrospective analysis has shown no benefit of eMT in minor stroke patients with isolated M2 occlusion over a more conservative therapeutic approach. Although our results must be viewed with caution, in these patients it appears reasonable to consider BMM as the first option and rMT in the presence of early neurological deterioration.
Calcitriol attenuates vascular remodeling in association with alteration of ppET-1/ETBR/eNOS and ETAR expression in acute and chronic phases of kidney ischemia–reperfusion injury in mice
Authors: Desita Eryna Ayu Nugra , Arfian Nur , Setyaningsih Wiwit Ananda Wahyu , Sari Dwi Cahyani Ratna 
Published: October 2022

Kidney ischemia–reperfusion injury (IRI) causes acute kidney injury with increasing risk of maladaptive repair through endothelin-1 (ET-1)/endothelin type A receptor (ETAR) signaling. Calcitriol shows renoprotection in kidney fibrosis, however, its effects on vasoactive substances expression and vascular remodeling following kidney IRI remain unclear. This research aimed to investigate Calcitriol’s effects on preproendothelin-1 ( ppET-1), ETAR, endothelial nitric oxide synthase ( eNOS) mRNA expression and vascular remodeling in acute and chronic phases of kidney IRI in mice. Twenty-five male Swiss mice were randomly divided into five groups ( n = 5): SO (sham-operated), IR3 (3 day kidney IRI), IR12 (12 day kidney IRI), IRD3 (3 day kidney IRI + Calcitriol 0.5 µg/kg body weight (BW)/day), and IRD12 (12 day kidney IRI + Calcitriol 0.5 µg/kg BW/day). Ischemia–reperfusion injury groups underwent bilateral renal pedicles clamping for 30 min, then reperfusion. Kidneys were harvested for Sirius Red staining to observe interstitial fibrosis and vascular remodeling, polymerase chain reaction to quantify ppET-1, endothelin type B receptor ( ETBR), eNOS mRNA expression, and Western blotting to quantify ETAR protein expression. Calcitriol treatment in both phases of kidney IRI showed lower serum creatinine and ETAR protein expression, while higher eNOS and ETBR mRNA expression than IRI-only groups. Furthermore, ppET-1 mRNA expression was higher in IRD3 than IR3, but lower in IRD12 than IR12. Calcitriol also prevented vascular remodeling as indicated by lower wall thickness and higher lumen/wall area ratio than IRI-only groups.
Investigation of the atomic structure of curium and determination of its first ionization potential
Authors: Kneip Nina , Weber Felix , Kaja Magdalena A. , Düllmann Christoph E. , Mokry Christoph , Raeder Sebastian , Runke Jörg , Studer Dominik , Trautmann Norbert , Wendt Klaus 
Published: October 2022

Abstract
We report on the investigation of the atomic structure of curium ($$Z=96$$

Z
=
96

) by resonance ionization spectroscopy. Three different excited energy levels were populated from the $$5f^{7}6d7s^{2}\,^{9}D^{o}_{2}$$

5

f
7

6
d
7

s
2

9

D
2
o

ground state as first excitation steps. Wide-range scans were performed for the search of second excitation steps around the literature value of the ionization potential. These spectra were analyzed to identify Rydberg levels and auto-ionizing resonances. The ionization potential was consistently determined as 48330.68(16)$$\hbox {cm} ^{-1}$$

cm

-
1

through the evaluation of Rydberg convergences and the complementary approach of DC electric field ionization by evaluating the ionization threshold according to the saddle point model. The new result deviates by 6.7$$\hbox {cm} ^{-1}$$

cm

-
1

from the literature value of 48324(2)$$\hbox {cm} ^{-1}$$

cm

-
1

by Köhler et al. [15] and is about one order of magnitude more precise.

Graphic abstract
The Benefit of Optimal Dietary Lipid Level for Black Seabream Acanthopagrus schlegelii Juveniles under Low-Salinity Environment
Authors: Jin Min , Li Xuejiao , Shen Yuedong , Bao Yangguang , Yang Bingqian , Wu Zhaoxun , Jiao Lefei , Zhou Qicun 
Published: October 2022

The present study was aimed at evaluating the regulatory effects of dietary lipid levels on growth performance, osmoregulation, fatty acid composition, lipid metabolism, and physiological response in Acanthopagrus schlegelii under low salinity (5 psu). An 8-week feeding trial was conducted in juvenile A. schlegelii with an initial weight of

2.27
±
0.05

g, and six isonitrogenous experimental diets were formulated with graded levels of lipid: 68.7 g/kg (D1), 111.7 g/kg (D2), 143.5 g/kg (D3), 188.9 g/kg (D4), 239.3 g/kg (D5), and 269.4 g/kg (D6), respectively. Results indicated that fish fed with diet containing 188.9 g/kg lipid significantly improved growth performance. Dietary D4 improved ion reabsorption and osmoregulation by increasing the concentrations of Na+, K+, and cortisol in serum and activities of Na+/K+-ATPase as well as expression levels of osmoregulation related to gene expression levels in the gill and intestine. The expression levels of long chain polyunsaturated fatty acid biosynthesis-related genes were dramatically upregulated when dietary lipid levels increased from 68.7 g/kg to 189.9 g/kg with levels of docosahexaenoic (DHA), eicosapentaenoic (EPA), and DHA/EPA ratio being highest in the D4 group. When fish fed dietary lipid levels from 68.7 g/kg to 188.9 g/kg, lipid homeostasis could be maintained by upregulating sirt1 and pparα expression levels, whereas lipid accumulation was observed in dietary lipid levels of 239.3 g/kg and over. Fish fed with high dietary lipid levels resulted in physiological stress related to oxidative stress and endoplasmic reticulum stress. In conclusion, based on weight gain, the optimal dietary lipid requirement of juvenile A. schlegelii reared at low-salinity water is 196.0 g/kg. These findings indicate that the optimal dietary lipid level can improve growth performance, n-3 LC-PUFA accumulation, and osmoregulatory ability and maintain lipid homeostasis and normal physiological functions of juvenile A. schlegelii.
Influence of Ginsenoside Rh2 on Cardiomyocyte Pyroptosis in Rats with Acute Myocardial Infarction
Authors: Song Wanmei , Dai Bin , Dai Yuntang 
Published: October 2022

Objective. This paper intends to verify through in vivo experiments whether ginsenoside Rh2 (G-Rh2) can play an anti-inflammatory role by modulating cardiomyocyte (CM) pyroptosis in rats with acute myocardial infarction (AMI), thereby alleviating myocardial injury. Methods. Twenty SD rats were randomized into control, L-Rh2, M-Rh2, and H-Rh2 groups, among which the latter three groups were modeled for AMI and given an intraperitoneal injection of G-Rh2 (L-Rh2: 2 mg/kg; M-Rh2: 4 mg/kg; H-Rh2: 8 mg/kg), while the control group was only treated with thoracotomy and sodium chloride injection. Heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), left ventricular systolic pressure (LVSP), and left ventricular end-diastolic pressure (LVEDP) were recorded by ultrasonic diagnosis. Rats were killed under anesthesia, and the morphological characteristics of ventricular tissue were observed by electron microscope. Additionally, cardiac blood and ventricular tissues were collected to quantify the contents of myocardial injury markers (creatine phosphate kinase (CK), creatine phosphokinase-MB isoenzyme (CK-MB), and lactate dehydrogenase (LDH) by ELISA, as well as the expression of pyroptosis-related genes cysteinyl aspartate specific proteinase 1 (Caspase-1), gasdermin D (GSDMD), interleukin (IL)-1β, and NOD-like receptor thermal protein domain associated protein 3 (NLRP3) by qRT-PCR and Western blot). Results. Ultrasonic examination identified lower HR, SBP, DBP, MAP, and LVSP in the three Rh2 injection groups compared with the control group (

P

< 0.05); and in comparison with M- and H-Rh2 groups, HR, SBP, DBP, MAP, and LVSP were all lower in L-Rh2 group, while LVEDP was higher (

P

< 0.05). Microscopically, CMs and organelles in the L-RH2, M-RH2, and H-RH2 groups were damaged to varying degrees compared with the control group, with those in the L-RH2 group being the most serious. CK, CK-MB, and LDH were also the highest in the L-Rh2 group and the lowest in the control group, while their levels were obviously reduced in M- and H-Rh2 groups (

P

< 0.05). Finally, GSDMD, IL-1β, NLRP3, and Caspase-1 were found to be reduced in the control group, while pyroptosis-related gene expression in the M-Rh2 group was improved markedly (

P

< 0.05). Conclusion. G-Rh2 can inhibit the pathological development of AMI by relieving the focal death of CM and inhibiting the release of proinflammatory factors in the body, and the effect is significantly related to the dosage, which is expected to become a new treatment option for AMI in the future.
Association between Poor Quality of Sleep and Metabolic Syndrome in Ghanaian University Students: A Cross-Sectional Study
Authors: Yeboah Kwame , Dodam Kennedy K. , Agyekum Jennifer A. , Oblitey Jared N. 
Published: October 2022

Aim. This study aimed to determine the association between quality of sleep and metabolic syndrome (MetS) and physical activity level in young adults at the University of Ghana. Method. In a cross-sectional design, 340 university students, aged between 20-30 years were recruited. Quality of sleep was assessed using the Pittsburgh Sleep Quality index (PSQI) and physical activity with the short form of the International Physical Activity Questionnaire (IPAQ-SF). Poor quality of sleep was defined as a global PSQI score>5 and low physical activity level as those not meeting the criteria for vigorous-moderate physical activity. Anthropometric features and blood pressures were measured, and fasting blood samples were collected from the participants to measure plasma levels of glucose, lipid profile, urea, and creatinine. MetS was defined using the Joint Scientific Statement criteria. Results. In our study population of young adults from Ghana, the prevalence of poor quality of sleep as measured by PSQI was 54.1%, and MetS was 12.4%. MetS was associated with poor quality of sleep in females [

OR

95
%
CI

=
2.11

(1.04–4.25),

p
=
0.038

] and entire study participants [2.18 (1.09–4.37)

p
=
0.029

] in both crude and adjusted models; no association was found in male participants. Low physical activity status was not associated with poor sleep status. Obesity [1.32 (1.02–3.56),

p
=
0.043

], but not overweight [0.99 (0.58–2.34),

p
=
0.862

], was associated with poor quality of sleep. Conclusion. Young adults in a Ghanaian university have a high prevalence of poor quality of sleep and is associated with MetS and obesity. Physical activity status was not associated with poor quality of sleep.
The Mutational Landscape of Cancer's Vulnerability to Ionizing Radiation
Authors: Gopal Priyanka , Yard Brian D. , Petty Aaron , Lal Jessica C. , Bera Titas K. , Hoang Trung Q. , Buhimschi Alexandru D. , Abazeed Mohamed E. 
Published: October 2022

Abstract

Purpose:
Large-scale sequencing efforts have established that cancer-associated genetic alterations are highly diverse, posing a challenge to the identification of variants that regulate complex phenotypes like radiation sensitivity. The impact of the vast majority of rare or common genetic variants on the sensitivity of cancers to radiotherapy remains largely unknown.

Experimental Design:
We developed a scalable gene editing and irradiation platform to assess the role of categories of variants in cells. Variants were prioritized on the basis of genotype–phenotype associations from a previously completed large-scale cancer cell line radiation profiling study. Altogether, 488 alleles (396 unique single-nucleotide variants) from 92 genes were generated and profiled in an immortalized lung cell line, BEAS-2B. We validated our results in other cell lines (TRT-HU1 and NCI-H520), in vivo via the use of both cell line and patient-derived murine xenografts, and in clinical cohorts.

Results:
We show that resistance to radiation is characterized by substantial inter- and intra-gene allelic variation. Some genes (e.g., KEAP1) demonstrated significant intragenic allelic variation in the magnitude of conferred resistance and other genes (e.g., CTNNB1) displayed both resistance and sensitivity in a protein domain-dependent manner. We combined results from our platform with gene expression and metabolite features and identified the upregulation of amino acid transporters that facilitate oxidative reductive capacity and cell-cycle deregulation as key regulators of radiation sensitivity.

Conclusions:
Our results reveal new insights into the genetic determinants of tumor sensitivity to radiotherapy and nominate a multitude of cancer mutations that are predicted to impact treatment efficacy.
Disabling Uncompetitive Inhibition of Oncogenic IDH Mutations Drives Acquired Resistance
Authors: Lyu Junhua , Liu Yuxuan , Gong Lihu , Chen Mingyi , Madanat Yazan F. , Zhang Yuannyu , Cai Feng , Gu Zhimin , Cao Hui , Kaphle Pranita , Kim Yoon Jung , Kalkan Fatma N. , Stephens Helen , Dickerson Kathryn E. , Ni Min , Chen Weina , Patel Prapti , Mims Alice S. , Borate Uma , Burd Amy , Cai Sheng F. , Yin C. Cameron , You M. James , Chung Stephen S. , Collins Robert H. , DeBerardinis Ralph J. , Liu Xin , Xu Jian 
Published: October 2022

Abstract

Mutations in IDH genes occur frequently in acute myeloid leukemia (AML) and other human cancers to generate the oncometabolite R-2HG. Allosteric inhibition of mutant IDH suppresses R-2HG production in a subset of patients with AML; however, acquired resistance emerges as a new challenge, and the underlying mechanisms remain incompletely understood. Here we establish isogenic leukemia cells containing common IDH oncogenic mutations by CRISPR base editing. By mutational scanning of IDH single amino acid variants in base-edited cells, we describe a repertoire of IDH second-site mutations responsible for therapy resistance through disabling uncompetitive enzyme inhibition. Recurrent mutations at NADPH binding sites within IDH heterodimers act in cis or trans to prevent the formation of stable enzyme–inhibitor complexes, restore R-2HG production in the presence of inhibitors, and drive therapy resistance in IDH-mutant AML cells and patients. We therefore uncover a new class of pathogenic mutations and mechanisms for acquired resistance to targeted cancer therapies.

Significance:
Comprehensive scanning of IDH single amino acid variants in base-edited leukemia cells uncovers recurrent mutations conferring resistance to IDH inhibition through disabling NADPH-dependent uncompetitive inhibition. Together with targeted sequencing, structural, and functional studies, we identify a new class of pathogenic mutations and mechanisms for acquired resistance to IDH-targeting cancer therapies.
This article is highlighted in the In This Issue feature, p. 1
Improving overlay maps of science: Combining overview and detail
Authors: Sjögårde Peter 
Published: October 2022

Abstract
Overlay maps of science are global base maps over which subsets of publications can be projected. Such maps can be used to monitor, explore, and study research through its publication output. Most maps of science, including overlay maps, are flat in the sense that they visualize research fields at one single level. Such maps generally fail to provide both overview and detail about the research being analyzed. The aim of this study is to improve overlay maps of science to provide both features in a single visualization. I created a map based on a hierarchical classification of publications, including broad disciplines for overview and more granular levels to incorporate detailed information. The classification was obtained by clustering articles in a citation network of about 17 million publication records in PubMed from 1995 onwards. The map emphasizes the hierarchical structure of the classification by visualizing both disciplines and the underlying specialties. To show how the visualization methodology can help getting both an overview of research and detailed information about its topical structure, I studied two cases: coronavirus/Covid-19 research and the university alliance called Stockholm Trio.
309 patients treated with fluoroscopy-guided caudal epidural injection for lumbar disc herniation
Authors: Akşan Özgür 
Published: October 2022

Objective To present our experience, discuss the complications, and assess early vs long-term outcomes of fluoroscopy-guided caudal epidural injection for lumbar disc herniation (LDH). Methods This was a prospective study of 309 patients who underwent fluoroscopy-guided caudal epidural injection from 2014 to 2020. The inclusion criteria were LDH diagnosis by magnetic resonance imaging, surgical treatment not required, age >18 years, and history of low back or leg pain despite >6 weeks of treatment comprising a combination of analgesics, anti-inflammatories, and physical therapy. The epidural injection solution comprised 8 mL of 0.5% bupivacaine hydrochloride (HCL), 2 mL dexamethasone, and 10 mL saline. Each patient completed a questionnaire comprising a visual analog scale (VAS) and the Back Pain Functional Scale (BPFS) at baseline, and 1 month and 1 year after injection. Results The VAS and BPFS scores indicated significantly less pain at 1 month and 1 year compared with the pre-procedure baseline values. Complications developed in 11 patients (reversible paresis in 7 patients, arrhythmia in 1 patient, headache in 1 patient, seizure in 1 patient, spondylodiscitis in 1 patient). Conclusions Fluoroscopy-guided caudal epidural injection for LDH is safe, and the procedure reduced pain and improved functional capacity compared with baseline.
Using Household Consumption Data to Flag Low Nutrient Access
Authors: Mathiassen Astrid , Lovon Margarita , Baille Barbara , Ogden Kathryn , Sandström Susanna 
Published: October 2022

Background: Information on food consumption, dietary diversity, and nutrient inadequacies are key for informing food security and nutrition programming. Household- and individual-level data together provide the most complete information, but individual dietary modules are not always feasible in humanitarian contexts due to cost and time constraints. Objective: This article asks to what extent it is possible to use food consumption data which is commonly collected at household level through food security and vulnerability surveys, to assess the household’s access to vitamin A and iron. Methods: The validation analysis uses household food consumption and expenditure surveys from Guatemala, Honduras, Nepal, and Uganda and the adult male equivalent approach for calculating nutrient access. Results: The results show a positive significant correlation between the frequency of consumption and adequacy as estimated from comprehensive household food consumption modules, with correlation in the range of 0.4 to 0.7. Frequency thresholds for distinguishing between adequate and inadequate nutrient access, based on how often foods rich in the relevant nutrient are eaten during 1 week, mostly fulfill standard sensitivity and specificity criteria. Conclusions: The article concludes that in humanitarian contexts, a frequency-based proxy for nutrient access based on household data commonly collected in emergency assessments and through monitoring systems can be used and can support this particular data gap. As a rule of thumb, a frequency threshold of 7 should be used for vitamin A and of 12 for iron.
Urbanity as a City's Heritage
Authors: Rautenberg Michel 
Published: October 2022

AbstractUrbanity has been a key concept to describe western cities since the beginning of the 20th century. But the importance of urbanity is no longer self-evident in global cities since the pivotal moment of 1980/90, when we witnessed the acceleration of flux, the emergence of megacities and the transformation of capitalism. At the same time, urban heritage became of greater interest. Then, as urbanity weakened, it began to be defended by public policies, local commitments and activists in a new way: as collective memories and palimpsest traces. In this article, I consider two examples in the French city of Saint-Etienne to illustrate the heritagization of urbanity: an exhibition about the memory of Algerian miners, and the dramatic demolition of a high-rise building that created a great stir because of its local symbolic significance. These two examples show that urbanity keeps a great place in the imaginary of urban dwellers, including the elites. From a general point of view, we try to preserve the values that we attach to cities, perhaps more than trying to preserve the cities themselves.