bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term survivors demonstrate superior quality of life after haploidentical stem cell transplantation to matched sibling donor transplantation
Authors: Zhang Xiaoyu , Wang Jiao , Liu Yuqiu , Liu Jie , Wang Bei , Zhang Qiuhui , Guan Wei , Zhang Huijuan , Xu Li , Liu Guiying , Zhang Ping , He Yi , Feng Sizhou , Han Mingzhe , Li Changping , Jiang Erlie , Xie Wenjun 
Published: October 2022

Abstract
Background: It has been well-documented that haplo-identical hematopoietic stem cell transplantation (HID-HSCT) can provide outcomes comparable to conventional matched sibling donor (MSD) HSCT, however, little is known about the effects on quality of life (QoL) in long-term survivors. This study is to investigate the differences in longitudinal recovery of QoL between HID and MSD HSCT using a comprehensive assessment system.
Methods: This prospective study enrolled consecutive patients who had received allogenic-HSCT (allo-HSCT) between January 2018 and December 2019 in our center. All patients were informed to complete QoL questionnaires including the Mos 36-Item Short-Form Health Survey (SF-36) and the Functional Assessment of Cancer Therapy Bone Marrow Transplant (FACT-BMT, version 4), using an online applet, before transplantation and at scheduled time points after transplantation. The linear mixed-effects modal was used to analyze the variation trend of different dimensions of both SF-36 and FACT-BMT with different follow-up times.
Results: Of the 425 participants, recipients of HID and MSD who survived more than 1 year (n = 230) were included in the final analysis of QoL (median age [range]: 36, [15,66]). The 3-year overall survival (OS) of HID and MSD was 82.42% and 86.46%, respectively. QoL was assessed using both SF-36 and FACT-BMT and there was longitudinal recovery with clinical significance in the cohort. Compared to MSD-HSCT patients, HID-HSCT recipients demonstrated superior QoL recovery in some subscales describing physical and mental wellness. Specifically, the difference in physical performance is more remarkable using FACT-BMT whereas that in mental wellness is more significant using SF36. In the subsequent stratified analysis, patients with a history of aGVHD or CMV reactivation demonstrated inferior QoL.
Conclusions and relevance: Long-term survivors of HID HSCT achieved better QoL in some sub-scales compared to MSD HSCT. In addition, SF-36 and FACT-BMT demonstrated different performance thus combination of both improved capacity of the evaluation system.
Research on the Win-win Performance of Carbon Dioxide Emissions Reduction and High-quality Development under the “Dual-carbon” Goal in China
Authors: Huaping Shi 
Published: October 2022

Abstract
As the country with the largest total and incremental carbon dioxide emissions in the world, China is under increasing international pressure to reduce carbon dioxide emissions. At present, China is in the post industrialization development stage, facing the dilemma of economic growth and carbon dioxide emissions reduction. Therefore, it is the most urgent task for the Chinese government to study the performance path of achieving a win-win situation of carbon dioxide emissions reduction and high-quality development under the “dual-carbon” goal. This paper calculates the comprehensive evaluation index of high-quality development in 30 provinces of China from 2005 to 2019, and uses the Spatial Dubin Model (SDM) to investigate the relationship between carbon dioxide emissions reduction and high-quality development. The research results show that there is a U-shaped nonlinear relationship between carbon dioxide emissions reduction and high-quality development, and the impact of carbon dioxide emissions reduction on high-quality development has significant spatial heterogeneity. Although carbon dioxide emission reduction policies will reduce economic growth in the short term, the Chinese government should strengthen carbon dioxide emission reduction policies in the long run.
HCN-channel-dependent hyperexcitability of the layer V pyramidal neurons in IL-mPFC contributes to fentanyl-induced hyperalgesia in male rats
Authors: Wang Xixi , Gan Sifei , Zhang Zeru , Zhu Pengfei , Li Chenhong , Luo Fang 
Published: October 2022

Abstract
Opioids are often first-line analgesics in pain therapy. However, prolonged use of opioids causes paradoxical pain, termed “opioid-induced hyperalgesia (OIH)”. The infralimbic medial prefrontal cortex (IL-mPFC) has been suggested to be critical in inflammatory and neuropathic pain processing through its dynamic output from Layer V pyramidal neurons. Whether OIH condition induces excitability changes of these output neurons and what mechanisms underlie these changes remains elusive. Here, with combination of patch-clamp recording, immunohistochemistry, as well as optogenetics, we revealed that IL-mPFC Layer V pyramidal neurons exhibited hyperexcitability together with higher input resistance. In line with this, optogenetic and chemogenetic activation of these neurons aggravate behavioral hyperalgesia in male OIH rats. Inhibition of these neurons alleviates hyperalgesia in male OIH rats but exerts an opposite effect in male control rats. Electrophysiological analysis of hyperpolarization-activated cation current (Ih) demonstrated that decreased Ih is a prerequisite for the hyperexcitability of IL-mPFC output neurons. This decreased Ih was accompanied by a decrease in HCN1, but not HCN2, immunolabeling, in these neurons. In contrast, the application of HCN channel blocker increased the hyperalgesia threshold of male OIH rats. Consequently, we identified an HCN-channel-dependent hyperexcitability of IL-mPFC output neurons, which governs the development and maintenance of OIH in male rats.
Chronic hypoparathyroidism is associated with increased cortical bone density evaluated by high-resolution peripheral quantitative computed tomography
Authors: Rosa Renata Gervais Santa , Polonine Simone , Pichone Alinie , Gomes Carlos Perez , Lima Luis Felipe Cardoso , Neto Francisco de Paula Paranhos , Mendonça Laura Maria Carvalho , Farias Maria Lucia Fleiuss , Madeira Miguel 
Published: October 2022

Abstract
Purpose
This cross-sectional study aimed to evaluate BMD, bone microarchitecture and prevalent fractures in women with chronic hypoparathyroidism (HypoPT).
Methods
Twenty-seven women with HypoPT and 44 age-matched healthy women were included. Dual-energy x-rays absorptiometry (DXA) was used to evaluate areal BMD (aBMD) at the spine and hip as well as morphometric vertebral fractures (VFA). Microarchitecture and volumetric BMD (vBMD) were evaluated at distal radius and tibia using high resolution peripheral quantitative computed tomography (HR-pQCT). Biochemical parameters, including FGF23 (fibroblast growth factor 23), CTX (C- terminal telopeptide of type I collagen) and P1NP (procollagen type I N-terminal propeptide), were measured. Previous low-impact fractures were also assessed, and the 10-year fracture risk was estimated using the FRAX tool for Brazilian population.
Results
There was a low risk of fractures on FRAX tool in all patients, and only two had morphometric vertebral fractures: one patient presented severe VF (T5) and moderate VF (T6); another patient had moderate spinal deformity at T8. No patient had a history of previous clinical fractures. Compared to controls, HypoPT women had higher aBMD in the lumbar spine (p = 0.030), femoral neck and total hip (p < 0.001) and higher cortical vBMD (p = 0.020 in the radius and p < 0.001 in the tibia). Trabecular bone was not affected. Both bone turnover markers P1NP and CTX were compatible with low turnover in the HypoPT patients. However, we found no statistically significant correlations between BMD and any biochemical data, including serum phosphate, CTX and P1NP.
Conclusions
There was a low prevalence of fragility fractures, compatible with the low fracture risk estimated by FRAX, in the twenty-seven HypoPT women studied. These patients had higher areal BMD and cortical vBMD as compared to controls, but the association with decreased bone turnover was not clear. Future longitudinal studies are needed to clarify the mechanisms involved in the bone fragility described in HypoPT patients.
Investigating the synergies between the Urban Heat Island (UHI) and Heatwaves (HWs)
Authors: Nadeem Faisal , Tariq Salman , Haq Zia Ul , Khan Hassan Saeed 
Published: October 2022

Abstract
South Asian cities are now experiencing high-intensity of heatwaves. Heatwaves (HWs) severely threaten human health, particularly in urbanized areas more susceptible to the urban heat island (UHI) effect. Due to climate change, heatwaves are becoming stronger, coupled with the intensity of urbanization, exacerbating the thermal risk for urbanites. Explored the synergistic interaction of UHI with HWs and non-HWs periods were investigated in Lahore city. Lahore's climate is subtropical, with relatively dry, moderate winters and extremely hot summers. The city has a distance of 2,179.55 mi (3,507.65 km) north of the equator. A strong relationship was observed between UHI and heatwaves. Land surface temperature (LST) measured by satellite is a widely used indicator for analyzing heatwaves. It has a wider spatial coverage compared to surface air temperature measured at weather stations. Measured the UHI intensity with both datasets. The UHI was analyzed during HWs and non-heatwaves using the heat index method, while SUHI intensity was analyzed using the MFOZs method. The LULC revealed the change in urban built-up class was 56.87% over the last 24 years. It was observed that a high population density zone has more urban heat island intensity. The peak average temperature difference during heatwave and non-heatwave was 5.15°C. During heatwaves, the difference in peak surface temperature was 14°C in the daytime and 6.74°C at night time.
Histological characteristics of macrodontic cheek teeth of the guinea pig
Authors: Ignaszak-Dziech Justyna , Kuropka Piotr , Piasecki Tomasz 
Published: October 2022

Abstract
Macrodontia is the enlargement of tooth dimensions of different ethology. The work aims to show a histological picture of macrodontic teeth in guinea pigs. The material was obtained from animals post mortem. 90 structural changes derived from 24 guinea pigs were evaluated. All teeth used in the study showed macrodontic changes. The samples were decalcified, dehydrated and embedded in paraffin. Material was cutted in the transverse and longitudinal plane in relation to the alveolar bone. Histological evaluation included apical bud cells, pulp cavity cells, periodontium, dentin, enamel, cementum and alveolar bone tissue. Individual elements were evaluated in respect of morphology and distribution. Moreover, the arrangement of the individual hard tooth structures was assessed on the teeth. No atypia of the cells that make up the apical bud as well as pulp and periodontal cavity was found. Displacement of periodontal cells or odontoblasts towards pulp cavity as well as disorganization of the cell system in the pulp cavity were observed. Changes in the dentine ligaments and the reconstruction of the alveolar bone were also observed in areas where dentine and cement systems were affected. It was observed on dental slides that the enamel is also involved in structural remodeling of the pulp cavity. Histological assessment led to the finding that structural changes in macrodontic teeth are made up of re-arranged typical tooth tissues.
The Heat is On: Temperature Increase Mimics Allosteric Signaling in Imidazole-Glycerol Phosphate Synthase
Authors: Maschietto Federica , Morzan Uriel , Tofoleanu Florentina , Gheeraert Aria , Chaudhuri Apala , Kyro Gregory , Nekrasov Peter , Brooks Bernard , Loria (Joseph) Pat , Rivalta Ivan , Batista Victor 
Published: October 2022

Abstract
Allosteric drugs have the potential to revolutionize biomedicine due to their enhanced selectivity and protection against overdosage. However, we need to better understand allosteric mechanisms in order to fully harness their potential in drug discovery. In this study, molecular dynamics simulations and nuclear magnetic resonance spectroscopy are used to investigate how increases in temperature affect allostery in imidazole glycerol phosphate synthase. Results demonstrate that temperature increase triggers a cascade of local amino acid-to-amino acid dynamics that remarkably resembles the allosteric activation that takes place upon effector binding. The differences in the allosteric response elicited by temperature increase as opposed to effector binding are conditional to the alterations of collective motions induced by either mode of activation. This work provides an atomistic picture of temperature-dependent allostery, which could be harnessed to more precisely control enzyme function.
The relevant research of adverse childhood experiences and “risky drinking” in children of alcoholics in China
Authors: Sun Guangqiang , Wu Tingfang , Huang Chengbing , Yu Mingchao , Guo Yan , Zhu Xihua , Yu Xin , Qiu Yujia 
Published: October 2022

Abstract
Objective: To determine whetheradverse childhood experiences (ACEs) of children of alcoholics (COA) were associated with their current “risky drinking”.
Methods: This case-control study used the Alcohol Use Disorder Identification Test (AUDIT, cutoff is 7) to divide the participants into two groups, a “risky drinking group" (N=53) and a "non-risky drinking group" (N=97). Demographic data, Adverse Childhood Experiences-International Questionnaire (ACE-IQ), the Hamilton Anxiety Rating Scale (HAMA), the Hamilton Depression Rating Scale (HAMD) and the Mini-International Neuropsychiatric Interview (MINI) were used for assessment. The specific relationships between ACEs and “risky drinking” were explored.
Results: Respondents ranged in age from 29.70±6.72 years; 74.5% were females; 94.7% were of Han nationality; 56.7% had a level of education above high school; 12% had no formal or stable job. The “risky drinking” group was more likely to have experienced a major depressive episode (P＜0.05), nonalcohol psychoactive substance use disorder and bulimia nervosa (P<0.01), and they also experienced more physical abuse(P＜0.05), community violence (P＜0.01) and collective violence (P＜0.05). In a single factor logistic regression, physical abuse, community violence and collective violence were associated with a two to eleven fold increase in “risky drinking” in the adult COA, and in multiple factor logistic regression, community violence showed a graded relationship with “risky drinking”.
Conclusion: The childhood adverse experiences contribute to “risky drinking” in COA. This finding in the Chinese context have significant implications for prevention not only in China but in other cultures. There must be greater awareness of the role of ACEs in the perpetuation of alcoholism.
First report of invasive Fall armyworm and African armyworm sharing the same maize plants in the field: implications for intra-guild competition
Authors: Carter R. , Gómez-Undiano I. , Rwezaauva P. , Florian F. , Mushobozi W. , Wilson K. 
Published: October 2022

Abstract
Both the native African armyworm (Spodoptera exempta Walker), AAW, and the invasive Fall armyworm (Spodoptera frugiperda J.E. Smith), FAW, attack maize in Africa. The invasion of FAW in Africa has created a scenario where these two Spodoptera species might co-occur and interact with each other. This could have implications for pest management, including insecticide resistance management, biological control and the effectiveness of management practices. Given the much more aggressive nature of FAW in comparison to AAW, we predicted that where the two species co-occur, FAW would dominate the intra-guild interaction. Our study includes field observations of armyworms on both Cynodon grasses and maize, including the systematic sampling of smallholder maize farms in Arusha, Tanzania. We found that FAW larvae and AAW larvae can occur in the same field and even on the same plant and leaf simultaneously however, statistically, their joint distributions are consistent with a non-competitive association.
Targeting the p53 pathway to treat Malignant Rhabdoid and Atypical Teratoid Rhabdoid Tumors
Authors: Roussel Martine , Cho Hyekyung , Stripay Jennifer , Refaat Alaa , Blankenship Kaley , Williams Justin , Mercer Kimberly , Connelly Michele , Ganguly Debolina , Robinson Sarah , Dhanda Sandeep , Stewart Clinton , Freeman Burgess , Roberts Charles , Tinkle Christopher , Stewart Elizabeth , Upadhyaya Santhosh , Shelat Anang 
Published: October 2022

Abstract
Rhabdoid tumors (RTs) predominantly affect young children and are among the deadliest pediatric solid tumors. Despite multimodal therapy consisting of surgery, radiation, and chemotherapy, children with these tumors have median survival of less than one year. RTs can arise throughout the body, including the central nervous system (CNS) where they are called atypical teratoid rhabdoid tumors (AT/RTs), and in extra-CNS locations such as the kidneys and other soft tissues where they are designated malignant RTs (MRTs). We previously identified MDM2 and MDM4 as therapeutic vulnerabilities in RTs and showed that treatment with the MDM2 inhibitor idasanutlin increased survival in mice bearing MRT xenografts. However, the therapeutic potential of idasanutlin in CNS RT tumors is unknown. Moreover, single agent therapies are prone to resistance and often show limited clinical benefit on their own. Therefore, we sought to identify combination strategies incorporating idasanutlin that would be effective in both CNS and extra-CNS RTs. Here, we show that the XPO1 inhibitor selinexor increased nuclear retention of p53 and potentiated idasanutlin-induced p53 pathway activation and cytotoxicity in AT/RT and MRT cell lines in vitro. Importantly, combination therapy limited acquired resistance through TP53 mutation. In vivo, combination therapy was well-tolerated, reduced tumor burden, and increased survival in orthotopic models of both AT/RT and MRT. Our results demonstrate that combining idasanutlin with selinexor is a promising therapeutic strategy for children with rhabdoid tumors.
Association between levels of exposure to heavy metals and renal function indicators of residents in environmentally vulnerable areas
Authors: Kwon Jung-Yeon , Lee Seungho , Surenbaatar Ulziikhishig , Lim Hyoun-Ju , Kim Byoung-Gwon , Eom Sang-Yong , Cho Yong Min , Kim Woo Jin , Yu Byeng-Chul , Lee Kwan , Hong Young-Seoub 
Published: October 2022

Abstract
Abandoned metal mines and refineries are considered environmentally vulnerable areas owing to high levels of exposure to heavy metals. This study examined the association between heavy metal exposure and renal function indicators. We studied a total of 298 participants, of which 74 and 68 resided in low- and high-exposure abandoned metal mine areas, respectively, with 121 in the refinery area and 35 in the control area. Blood and urine samples were collected from the participants to analyze the levels of blood lead, cadmium, and creatinine and urinary cadmium, NAG, and β2-MG. The estimated glomerular filtration rate, which is calculated using the Chronic Kidney Disease Epidemiology Collaboration equation, was used for assessments.
The study participants comprised more females than males, and their mean age was 70.3 years. The blood lead and cadmium as well as urinary cadmium levels were 2.12 μg/dL, 1.89 μg/L, and 2.11 μg/L, respectively, in the heavy metal-exposure areas, and 1.18 μg/dL, 0.89 μg/L, and 1.11 μg/L, respectively, in the control area. The odds ratio (OR) for exceeding the reference value showed that blood cadmium in the refinery area was 38 times higher than that in the control area. Urinary cadmium was seven times higher in the low-exposure abandoned metal mine area than in the control area. NAG showed a positive correlation with urinary cadmium in all areas. In the refinery area, correlations were observed between β2-MG and urinary cadmium levels and the eGFR and blood cadmium level; in the high-exposure abandoned metal mine area, correlations were observed between NAG, β2-MG, and the eGFR and blood cadmium.
In this study, the association between Cd exposure and some renal function indicators was observed. This study’s findings and the obtained biological samples can serve as a basis for future molecular biological research.
Real feelings and experiences of obesity patients in metabolic surgery of weight loss in Shandong,China: A qualitative research
Authors: YANG Hailing , Zhao Lingling , Wang Chao , Zheng Xing , Sun Chunhua , LI Lihua 
Published: October 2022

Abstract
Background: This was to investigate the real feelings and experiences of losing weight after metabolic surgery of weight loss in obesity patients.
Methods: A qualitative study using focus groups was undertaken at Qilu Hospital of Shandong University,China. After obtaining ethical permission and informed consent of the interviewees, semi-structured interviews were conducted on 15 obese patients who underwent metabolic surgery for weight loss. The NVIVO 12 software was used to analyze the data using the Colaizzi 7-step method.
Results: 1) morbid obesity complications and severity of complications, awareness of weight loss surgery, family and peer support, and access to professional hospital information all have an impact on patients' surgical decisions. 2) insufficient knowledge of surgical weight loss leads to fear of surgery and the discomforts of obesity complications aggravate preoperative anxiety. The unfamiliar feelings in the intensive care unit after surgery and discomfort in tubing and physical restraint lead to a negative experience while the intimacy of medical staff visiting bedside and the joy of physical improvement help improve mood. 3)insufficient self-management confidence, and eagerly hope that medical staff continues to care. 4) pay attention to the weight management of relatives, friends and the next generation. And be willing to actively help others to share their experiences and scientifically reduce weight.
Conclusion: Medical staffs and patients should pay more attention to self-management and strengthen the health knowledge, peer support and extended care after discharge.
ELAC2 functions as a key gene in the early development of placental formation based on WGCNA
Authors: Liang Junyong , Liang Jingjie , Tan Qiang , Chen Kaiyu , Wang Zhengguang 
Published: October 2022

Abstract
Placenta plays a crucial role in mammalian fetal growth. The most important cell type in the placenta is the trophoblast cell. Many genes have been reported to play important functions in the differentiation of early placental trophoblast cells. Weighted gene co-expression network analysis (WGCNA) is a systematic biological method for describing the correlation patterns among genes across microarray samples. We used WGCNA to screen placental trophoblast development-related genes and through experimental confirmation, we showed that among these genes, ELAC2 may play an important regulatory role in the early development of mammalian placental formation. ELAC2 regulates early placental trophoblast differentiation by affecting cell migration and cell proliferation. In addition, ELAC2 may be involved in regulating cell migration processes in a manner that affects epithelial mesenchymal transition (EMT).