bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression ofscp3anddazlreveals the meiotic characteristics of the olive flounderParalichthys olivaceus
Authors: Wang Wenxiang , Liang Shaoshuai , Zou Yuxia , Li Ze , Wu Qiaowan , Wang Lijuan , Wu Zhihao , Peng Zhuangzhuang , You Feng 
Published: October 2022

AbstractOlive flounder Paralichthys olivaceus is an important cultured marine fish. We found that the meiosis marker scp3 and its intrinsic regulator dazl were mainly expressed in the gonads. During the ovarian differentiation, scp3 signal was detected first in pre-meiotic oogonia at 60-mm total length (TL) and then in primary oocytes at 80- and 100-mm TL, with a sharp increase in scp3 expression level observed at 80- and 100-mm TL. Dazl signal was detected in primordial germ cells at 30-mm TL and oogonia at 60-mm TL, but no significant change of expression was observed. During the testicular differentiation period, scp3 and dazl expression remained at low levels, and scp3 signal was weakly detected in spermatogonia at 80-mm TL, whereas dazl signal was not found. During the ovarian developmental stages, the highest expression levels of scp3 and dazl were detected at stages I and II, respectively, and strong signals of scp3 and dazl were detected in primary oocytes and oocytes at phases I and II. In the testis, the high expression of scp3 and dazl was detected at stages II–IV and II–III, respectively. Scp3 signal was weakly observed in pre-meiotic spermatogonia at stages I and II and strongly detected in primary spermatocytes at stages III–V. Dazl was detected in the nuclei of spermatogonia and spermatids at stages II–IV. Furthermore, scp3 expression in the ovary could be promoted by 17α-ethynylestradiol and tamoxifen, whereas dazl expression could be downregulated by tamoxifen.
The molecular chaperone cysteine string protein is required for monomeric SNARE proteins to assemble intrans-complexes during human sperm acrosomal exocytosis
Authors: Flores-Montero Karina , Berberián María Victoria , Mayorga Luis Segundo , Tomes Claudia Nora , Ruete María Celeste 
Published: October 2022

AbstractMembrane fusion in sperm cells is crucial for acrosomal exocytosis and must be preserved to ensure fertilizing capacity. Evolutionarily conserved protein machinery regulates acrosomal exocytosis. Molecular chaperones play a vital role in spermatogenesis and post-testicular maturation. Cysteine string protein (CSP) is a member of the Hsp40 co-chaperones, and the participation of molecular chaperones in acrosomal exocytosis is poorly understood. In particular, the role of CSP in acrosomal exocytosis has not been reported so far. Using western blot and indirect immunofluorescence, we show that CSP is present in human sperm, is palmitoylated, and predominantly bound to membranes. Moreover, using functional assays and transmission electron microscopy, we report that blocking the function of CSP avoided the assembly of trans-complexes and inhibited exocytosis. In summary, here, we describe the presence of CSP in human sperm and show that this protein has an essential role in membrane fusion during acrosomal exocytosis mediating the trans-SNARE complex assembly between the outer acrosomal and plasma membranes. In general, understanding CSP’s role is critical in identifying new biomarkers and generating new rational-based approaches to treat male infertility.
On the implied weights of linear regression for causal inference
Authors: Chattopadhyay Ambarish , Zubizarreta José R 
Published: October 2022

Summary
A basic principle in the design of observational studies is to approximate the randomized experiment that would have been conducted under ideal circumstances. At present, linear regression models are commonly used to analyse observational data and estimate causal effects. How do linear regression adjustments in observational studies emulate key features of randomized experiments, such as covariate balance, self-weighted sampling and study representativeness? In this paper, we provide answers to this and related questions by analysing the implied individual-level data weights of various linear regression methods. We derive new closed-form expressions of these implied weights, and examine their properties in both finite and large samples. Among others, in finite samples we characterize the implied target population of linear regression, and in large samples demonstrate the multiply robust properties of regression estimators from the perspective of their implied weights. We show that the implied weights of general regression methods can be equivalently obtained by solving a convex optimization problem. This equivalence allows us to bridge ideas from the regression modelling and causal inference literatures. As a result, we propose novel regression diagnostics for causal inference that are part of the design stage of an observational study. We implement the weights and diagnostics in the new lmw package for R.
Acute and chronic kidney disease and risk of hospital mortality during COVID-19 pandemic waves in the pre-vaccination era
Authors: Portolés José , López-Sánchez Paula , Martin-Rodríguez Leyre , Serrano-Salazar María Luisa , Valdenebro-Recio Maria , Ramos Antonio , Malo Rosa María , Zalamea Felipe , Martin-Giner Juan Manuel , Marques María , Ortiz Alberto 
Published: October 2022

ABSTRACT

Background
Chronic kidney disease (CKD) is a risk factor for death from coronavirus disease 2019 (COVID-19), and COVID-19 may cause acute kidney injury (AKI) which also influences outcomes. There is little information on the independent contribution of CKD and AKI to the risk of death in COVID-19 on different waves, as CKD is a key risk factor for AKI.

Methods
We have studied the epidemiology of CKD and AKI in 2878 patients hospitalized for COVID-19 and their independent association with in-hospital mortality in the two largest pre-vaccination COVID-19 waves in Madrid, Spain. Hospitalized COVID-19 patients were grouped into four mutually exclusive categories: previous-CKD, community-acquired AKI (CA-AKI), hospital-acquired AKI (HA-AKI) and normal renal function throughout hospitalization.

Results
Pre-existent or acquired kidney involvement was observed in 35.5% and 36.8% of COVID-19 patients in the 1st and 3rd waves, respectively. Overall, 13.9% of patients with normal kidney function on arrival developed HA-AKI. In the 3rd wave, CA-AKI was more common than in the 1st wave. Overall, 9%–20% of CKD cases and 22%–40% of AKI cases remained undiagnosed in the discharge report. CKD, CA-AKI and HA-AKI were independently associated with risk of death in multivariate analysis, with HA-AKI, which was usually mild, being the most relevant independent risk factor for in-hospital mortality. A model including kidney involvement category, age, Charlson index, admission lactate dehydrogenase and lymphocytes predicted death with a receiver operating characteristic area under the curve of 0.898.

Conclusion
In conclusion, CKD and AKI were common in pre-vaccination waves among hospitalized COVID-19 patients and were independent risk factors for death, even when AKI was mild to moderate, and despite improvements in treatment.
Ceramides and Phosphatidylcholines Associate with Cardiovascular Diseases in the Elderly
Authors: Katajamäki Taina T , Koivula Marja-Kaisa , Hilvo Mika , Lääperi Mitja T A , Salminen Marika J , Viljanen Anna M , Heikkilä Elisa T M , Löppönen Minna K , Isoaho Raimo E , Kivelä Sirkka-Liisa , Jylhä Antti , Viikari Laura , Irjala Kerttu M , Pulkki Kari J , Laaksonen Reijo M H 
Published: October 2022

AbstractBackgroundThe ceramide- and phospholipid-based cardiovascular risk score (CERT2) has been found to predict the risk for cardiovascular disease (CVD) events, especially cardiovascular mortality. In the present study, our aim was to estimate the predictive ability of CERT2 for mortality of CVD, coronary artery disease (CAD), and stroke in the elderly and to compare these results with those of conventional lipids.MethodsWe conducted a prospective study with an 18-year follow-up period that included a total of 1260 participants ages ≥64 years. Ceramides and phosphatidylcholines were analyzed using a LC-MS. Total cholesterol and triglycerides were performed by enzymatic methods and HDL cholesterol was determined by a direct enzymatic method. Concentrations of LDL-cholesterol were calculated according to the Friedewald formula.ResultsA higher score of CERT2 was significantly associated with higher CVD, CAD, and stroke mortality during the 18-year follow-up both in unadjusted and adjusted Cox regression models. The unadjusted hazard ratios (HRs) of CERT2 (95% CI) per SD for CVD, CAD, and stroke were 1.72 (1.52–1.96), 1.76 (1.52–2.04), and 1.63 (1.27–2.10), respectively, and the corresponding adjusted HRs (95% CI) per SD for CERT2 were 1.48 (1.29–1.69), 1.50 (1.28–1.75), and 1.41 (1.09–1.83). For conventional lipids, HRs per SD were lower than for CERT2.ConclusionsThe risk score CERT2 associated strongly with CVD, CAD, and stroke mortality in the elderly, while the association between these events and conventional lipids was weak.
Online/Offline Attribute-Based Boolean Keyword Search For Internet Of Things
Authors: Yao Jiahui , Xu Lingling 
Published: October 2022

Abstract
With the rapid development of Internet of Things (IoT) and cloud computing, a large amount of IoT data has been stored and shared in cloud servers. However, sensitive IoT data may be leaked by untrusted cloud servers, which is a key problem hindering the development of IoT-cloud systems. To solve this problem, searchable encryption has been presented, which enables IoT devices to encrypt the collected data before uploading them to the cloud and make retrievals over the encrypted data by keyword queries. In this paper, we will study attribute-based keyword search (ABKS), which relieves us from cumbersome key management and provides a fine-grained access control. However, since the existing ABKS schemes are all constructed by bilinear pairings, which incurs high computational costs, they are not suitable for resource-constrained IoT devices. In this article, we propose an online/offline attribute-based boolean keyword search scheme, which can significantly reduce the online computing costs of IoT devices. In addition, our scheme can support boolean keyword search for data users, which has more flexible keyword expressivity than the existing online/offline ABKS schemes. We simulate our scheme and the experimental results show that it is both efficient and practical for the real IoT-cloud systems.
The year 2021 in the European Heart Journal—Cardiovascular Imaging: Part I
Authors: Edvardsen Thor , Donal Erwan , Muraru Denisa , Gimelli Alessia , Fontes-Carvalho Ricardo , Maurer Gerald , Petersen Steffen E , Cosyns Bernard 
Published: October 2022

Abstract
The European Heart Journal—Cardiovascular Imaging was introduced in 2012 and has during these 10 years become one of the leading multimodality cardiovascular imaging journals. The journal is currently ranked as Number 19 among all cardiovascular journals. It has an impressive impact factor of 9.130 and our journal is well established as one of the top cardiovascular journals. The most important studies published in our Journal in 2021 will be highlighted in two reports. Part I of the review will focus on studies about myocardial function and risk prediction, myocardial ischaemia, and emerging techniques in cardiovascular imaging, while Part II will focus on valvular heart disease, heart failure, cardiomyopathies, and congenital heart disease.
A multicentre, propensity score matched analysis comparing a valve-sparing approach to valve replacement in aortic root aneurysm: Insight from the AVIATOR database
Authors: Arabkhani Bardia , Klautz Robert J M , de Heer Frederiek , De Kerchove Laurent , El Khoury Gebrine , Lansac Emmanuel , Schäfers Hans-Joachim , El-Hamamsy Ismail , Lenoir Marien , Aramendi José I , Meuris Bart , Verbrugghe Peter , Kluin Jolanda , Koolbergen Dave R , Bouchot Olivier , Rudez Igor , Kolesar Adrian , van Brakel Thomas J 
Published: October 2022

AbstractOBJECTIVESOur goal was to evaluate the outcome of valve-sparing root replacement (VSRR) and to compare the outcomes to those of patients having composite valve–graft conduit aortic root replacement (CVG-ARR) in a cohort of patients with aortic root aneurysm ± valve insufficiency, without valvular stenosis. Although valve-sparing procedures are preferable in young patients, there is a lack of comparative data in comparable patients.METHODSThe VSRR procedures were performed in 2005 patients, and 218 patients underwent a CVG-ARR procedure. Exclusion criteria included aortic dissection, endocarditis and valvular stenosis. Propensity score matching (3:1 ratio) was applied to compare VSRR (reimplantation 33% and remodelling 67%) and CVG-ARR.RESULTSWe matched 218 patients with CVG-ARR to 654 patients with VSRR (median age, 56.0; median follow-up was 4 years in both groups; interquartile range 1–5 years). Early mortality was 1.1% of those who had VSRR versus 2.3% in those who had CVG-ARR. Survival was 95.4% [95% confidence interval (CI) 94–97%] at 5 years in patients who had VSRR versus 85.4% (95% CI 82–92%) in those who had CVG-ARR; P = 0.002. Freedom from reintervention at 5 years was 96.8% (95% CI 95–98%) with VSRR and 95.4% (95% CI 91–99%) with CVG-ARR (P = 0.98). Additionally, there were more thromboembolic, endocarditis and bleeding events in the patients who had CVG-ARR (P = 0.02).CONCLUSIONSThis multicentre study shows excellent results after valve-sparing root replacement in patients with an ascending aortic aneurysm with or without valve insufficiency. Compared to composite valve-graft aortic root replacement, survival is better and valve-related events are fewer. Consequently, valve-sparing procedures should be considered whenever a durable repair is feasible. We advocate a valve-sparing strategy even in more complex cases when performed in experienced centres.
Identifying Cognitive Impairment Among Older Participants in a Nationally Representative Internet Panel
Authors: Gatz Margaret , Schneider Stefan , Meijer Erik , Darling Jill E , Orriens Bart , Liu Ying , Kapteyn Arie 
Published: October 2022

AbstractObjectivesThe Health and Retirement Study Telephone Interview for Cognitive Status (HRS TICS) score and its associated Langa–Weir cutoffs are widely used as indicators of cognitive status for research purposes in population-based studies. The classification is based on in-person and phone interviews of older individuals. Our purpose was to develop a corresponding classification for web-based self-administered assessments.MethodsParticipants were 925 members of a nationally representative internet panel, all aged 50 and older. We conducted (a) a phone interview comprised of cognitive items used to construct the HRS TICS score, and (b) a web counterpart with self-administered cognitive items, while also considering (c) other already administered web-based cognitive tests and instrumental activities of daily living survey questions, all from the same respondents.ResultsThe web-administered HRS TICS items have only modest correlations with the same phone items, although neither mode showed universally higher scores than the other. Using latent variable modeling, we created a probability of cognitive impairment score for the web-based battery that achieved good correspondence to the phone Langa–Weir classification.DiscussionThe results permit analyses of predictors, correlates, and consequences of cognitive impairment in web surveys where relevant cognitive test and functional abilities items are available. We discuss challenges and caveats that may affect the findings.
Trans-ancestry, Bayesian meta-analysis discovers 20 novel risk loci for inflammatory bowel disease in an African American, East Asian and European cohort
Authors: Cordero Roberto Y , Cordero Jennifer B , Stiemke Andrew B , Datta Lisa W , Buyske Steven , Kugathasan Subra , McGovern Dermot P B , Brant Steven R , Simpson Claire L 
Published: October 2022

AbstractInflammatory bowel disease (IBD) is an immune-mediated chronic intestinal disorder with major phenotypes: ulcerative colitis (UC) and Crohn’s disease (CD). Multiple studies have identified over 240 IBD susceptibility loci. However, most studies have centered on European (EUR) and East Asian (EAS) populations. The prevalence of IBD in non-EUR, including African Americans (AAs), has risen in recent years. Here we present the first attempt to identify loci in AAs using a trans-ancestry Bayesian approach (MANTRA) accounting for heterogeneity between diverse ancestries while allowing for the similarity between closely related populations. We meta-analyzed genome-wide association studies (GWAS) and Immunochip data from a 2015 EUR meta-analysis of 38 155 IBD cases and 48 485 controls and EAS Immunochip study of 2824 IBD cases and 3719 controls, and our recent AA IBD GWAS of 2345 cases and 5002 controls. Across the major IBD phenotypes, we found significant evidence for 92% of 205 loci lead SNPs from the 2015 meta-analysis, but also for three IBD loci only established in latter studies. We detected 20 novel loci, all containing immunity-related genes or genes with other evidence for IBD or immune-mediated disease relevance: PLEKHG5;TNFSFR25 (encoding death receptor 3, receptor for TNFSF15 gene product TL1A), XKR6, ELMO1, BC021024;PI4KB;PSMD4 and APLP1 for IBD; AUTS2, XKR6, OSER1, TET2;AK094561, BCAP29 and APLP1 for CD; and GABBR1;MOG, DQ570892, SPDEF;ILRUN, SMARCE1;CCR7;KRT222;KRT24;KRT25, ANKS1A;TCP11, IL7, LRRC18;WDFY4, XKR6 and TNFSF4 for UC. Our study highlights the value of combining low-powered genomic studies from understudied populations of diverse ancestral backgrounds together with a high-powered study to enable novel locus discovery, including potentially important therapeutic IBD gene targets.
Temperature and upwelling dynamics drive market squid (Doryteuthis opalescens) distribution and abundance in the California Current
Authors: Suca Justin J , Santora Jarrod A , Field John C , Curtis K Alexandra , Muhling Barbara A , Cimino Megan A , Hazen Elliott L , Bograd Steven J 
Published: October 2022

Abstract
Market squid (Doryteuthis opalescens) constitute one of California’s largest fisheries by landings and are an essential prey item for numerous top predators in the California Current ecosystem. However, extreme fluctuations in market squid abundance inhibit our ability to forecast local recruitment. We generate a species distribution model for market squid with data from an annual survey to examine the mechanisms behind the variability in their regional abundance and occurrence. Our results indicate juvenile market squid abundance was controlled by local recruitment in connection with sea surface temperature and upwelling dynamics, with finer spatial variability connected to the extent of upwelling dominated regions. Recent changes in these environmental factors also appear to contribute to the recent northward range expansion of market squid. Our spatiotemporally explicit model estimates of juvenile market squid abundance predicted the occurrence and abundance of older market squid in the diet of California sea lions (Zalophus californianus) and California fishery landings with as much skill as regional survey indices, suggesting the models are robust. Collectively, we provide mechanisms driving market squid variability throughout California’s waters and an ecosystem assessment of this economically and ecologically critical species.
Population projections of Pacific sardine driven by ocean warming and changing food availability in the California Current
Authors: Koenigstein Stefan , Jacox Michael G , Pozo Buil Mercedes , Fiechter Jerome , Muhling Barbara A , Brodie Stephanie , Kuriyama Peter T , Auth Toby D , Hazen Elliott L , Bograd Steven J , Tommasi Desiree 
Published: October 2022

Abstract
Small pelagic fish are important marine ecosystem components and highly variable fisheries resources. In the California Current upwelling system, Pacific sardine (Sardinops sagax) has supported important fisheries in the past, but contrary to expectations, remains at low biomass despite recent warm ocean conditions. We developed a data-driven, process-based population model that reproduces fluctuations of the US Pacific sardine population based on ocean temperature, early life stage and adult food, and upwelling strength. The lack of sardine recovery after 2014 can be explained by reduced food availability. Ensemble projections for the 21st century driven by downscaled ocean-biogeochemical simulations under three Earth system models (ESMs) show a likely recovery to early 2000s sardine abundance and catch by mid-century, due to increased recruitment. Ecological process uncertainty (ensemble configuration range) is of the same magnitude as uncertainty among ESM projections, and uncertainty related to the thermal optimum of early life stages dominates after 2070. Even for a fish species presumably favoured by warmer conditions, future climate projections entail risks of stock declines in food-limited years and when passing unknown thermal optima. Quantification of combined environmental driver impacts and sources of uncertainty to projections under novel conditions open new pathways for environment-responsive fisheries management strategies.
Acoustic disturbance in blue mussels: sound-induced valve closure varies with pulse train speed but does not affect phytoplankton clearance rate
Authors: Hubert Jeroen , Moens Rosalie , Witbaard Rob , Slabbekoorn Hans 
Published: October 2022

Abstract
Anthropogenic sound has increasingly become part of the marine soundscape and may negatively affect animals across all taxa. Invertebrates, including bivalves, received limited attention even though they make up a significant part of the marine biomass and are very important for higher trophic levels. Behavioural studies are critical to evaluate individual and potentially population-level impacts of noise and can be used to compare the effects of different sounds. In the current study, we examined the effect of impulsive sounds with different pulse rates on the valve gape behaviour and phytoplankton clearance rate of blue mussels (Mytilus spp.). We monitored the mussels’ valve gape using an electromagnetic valve gape monitor and their clearance rate using spectrophotometry of phytoplankton densities in the water. We found that the mussels’ valve gape was positively correlated with their clearance rate, but the sound exposure did not significantly affect the clearance rate or reduce the valve gape of the mussels. They did close their valves upon the onset of a pulse train, but the majority of the individuals recovered to pre-exposure valve gape levels during the exposure. Individuals that were exposed to faster pulse trains returned to their baseline valve gape faster. Our results show that different sound exposures can affect animals differently, which should be taken into account for noise pollution impact assessments and mitigation measures.
A review on the research progress of mechanical meta-structures and their applications in rail transit
Authors: Zhang Linli , Sheng Xiaozhen 
Published: October 2022

Abstract
Due to their light weight and excellent performance on vibration and noise control, novel mechanical meta-structures are exhibiting increasingly extensive application prospects in engineering. The structures also have great potential to meet the development requirements of high safety, light weight, and low noise in the field of rail transit. Mechanical meta-structures include acoustic black holes, phononic crystals, and mechanical/acoustic metamaterials, with which special mechanical properties that are not available to traditional structures can be achieved through innovative design of the structural units. In this paper, the fundamental properties and research progress of these meta-structures are described and their prospective use in the rail transit field are explored based on their applications on vibration control and noise reduction. It is hoped that this paper can provide some useful references for relevant researchers and engineers.