bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sleep deprivation induces dopamine system maladaptation and escalated corticotrophin-releasing factor signaling in adolescent mice
Authors: Tuan Li-Heng , Yeh Jin-Wei , Lee Lukas Jyuhn-Hsiarn , Lee Li-Jen 
Published: June 2022

Abstract
Sleep disruption is highly associated with the pathogenesis and progression of a wild range of psychiatric disorders. Furthermore, appreciable evidence shows that experimental sleep deprivation (SD) on humans and rodents evokes anomalies in the dopaminergic (DA) signaling, which are also implicated in the development of psychiatric illnesses such as schizophrenia or substance abuse. Since adolescence is a vital period for the maturation of the DA system as well as the occurrence of mental disorders, the present studies aimed to investigate the impacts of SD on the DA system of adolescent mice. We found that 72 h SD elicited a hyperdopaminergic status, with increased sensitivity to the novel environment and Amphetamine (Amph) challenge. Also, altered neuronal activity and expression of striatal DA receptors were noticed in the SD mice. Moreover, 72 h SD influenced the immune status in the striatum, with reduced microglial phagocytic capacity, primed microglial activation, and neuroinflammation. The abnormal neuronal and microglial activity were putatively provoked by the enhanced corticotrophin-releasing factor (CRF) signaling and sensitivity during the SD period. Together, our findings demonstrated the consequences of SD in adolescents including aberrant neuroendocrine, DA system, and inflammatory status. Sleep insufficiency is a risk factor for the aberration and neuropathology of psychiatric disorders.
Class dynamics and relationship between land-use systems and surface temperature in South-eastern Ghana
Authors: Sarfo Isaac , Bi Shuoben , Kwang Clement , Yeboah Emmanuel , Addai Foster Kofi , Nkunzimana Athanase , Ofori Meshack Appiah , Batame Michael , Appea Emmanuella Aboagye , Asiedu Aaron 
Published: June 2022

Abstract
A fifty-year study of land cover changes in Ghana’s south-eastern region is conducted to identify major shifts and new avenues for justifiable growth and development. A combination of early studies and geospatial technology was adapted to analyse the data. Our study investigated the relationship between some given variables using autocorrelation and geoinformatics. Socio-political and economic factors have caused a massive shift in the land cover through growth in human settlements, migration, the extensiveness of agriculture, poverty, unregulated and unreported logging of trees, small-scale mining intensification, and weak governance systems. We discovered that the natural vegetation had dramatically reduced (-52.01%), whilst built-up (+ 406.05%), farmlands/shrubs (+ 86.47%), and waterbodies (+ 47.35) were faced with a drastic increase. Class contribution rates show built environment massively influenced land modification, compared to other surveyed classes. In light of the major influences observed, it can be concluded that dynamics in land-use systems caused a substantial drift in local temperature. Temperature and built-up index correlated strongly (R2 = 0.959, p < .0001), whilst depicting a negative association against vegetation (R2 = 0.959, p < .0001) and water-index (R2 = 0.958, p < .0001). Variance assessment proved that the linear correlation model for the understudied elements is appropriate. The study provides policy guidance and basic research support to key proponents and practitioners in addressing undesirable consequences, caused by urbanization, public behaviour or lifestyles, and institutional failures.
Trends in Psychiatric Disorders by COVID-19 Infection and Hospitalization in 62 Counties in New York City from March 2020, to August 2021
Authors: Xiao Yunyu , Sharma Mohit , Thiruvalluru Rohith Kumar , Gimbrone Catherine , Keyes Katherine , Pathak Jyotishman 
Published: June 2022

Abstract
Determining emerging trends of different psychiatric disorders among patients with SARS-CoV-2 infection and subsequent hospitalization by psychiatric disorder history is important to guide targeted public health priorities. This study used electronic health records among patients in New York derived from the largest health information exchange in the US and estimated the prevalence of psychiatric between March 2020 to August 2021. During the beginning of the COVID-19 pandemic, mood disorders were the most prevalent among hospitalized COVID-19 patients, whereas substance use disorders increased significantly among patients without prior psychiatric diseases. Mood disorders, anxiety disorders and psychosis increased by 488.0% (from 2.5–14.7%), 373.2% (from 2.6–12.3%) 232.4% (from 3.7–12.3%) from February to August 2021 among COVID-19 negative patients. This study is the first to address the differential trends in psychiatric disorders over COVID-19 pandemic. Tailored treatment and policies should be developed that account for psychiatry history.
Bacterial production and biophysical characterization of a hard-to-fold scFv against myeloid leukemia cell surface marker, IL-1RAP
Authors: Farokhi-Fard Aref , Bayat Elham , Parikhani Arezoo Beig , Komijani Samira , Aliabadi Hooman Aghamirza Moghim , Sardari Soroush , Gharib Behrouz , Barkhordari Farzaneh , Azadmanesh Kayhan , Karimipoor Morteza , Bakhshandeh Haleh , Davami Fatemeh 
Published: June 2022

Abstract
Background: Interleukin-1 receptor accessory protein (IL-1RAP) is one of the most promising therapeutic targets proposed for myeloid leukemia. Antibodies (Abs) specific to IL-1RAP could be valuable tools for targeted therapy of this lethal malignancy. This study is about the preparation of a difficult-to-produce single-chain variable fragment (scFv) construct against membrane-bound isoform of human IL-1RAP using Escherichia coli (E. coli).Methods: Different approaches were examined for refolding and characterization of the scFv. Binding activities of antibody fragments were comparatively evaluated using cell-based enzyme-linked immunosorbent assay (ELISA). Homogeneity and secondary structure of selected scFv preparation were analyzed using analytical size exclusion chromatography (SEC) and circular dichroism (CD) spectroscopy, respectively. The activity of the selected preparation was evaluated after long-term storage, repeated freeze-thaw cycles, or following incubation with normal and leukemic serum. The probable anti-proliferation activity against leukemic cells was also evaluated.Results: Strategies for soluble expression of the scFv failed. Even with the help of Trx, ≥98% of proteins were expressed as inclusion bodies (IBs). Among three different refolding methods, the highest recovery rate was obtained from the dilution method (11.2%). Trx-tag substantially enhanced the expression level (˃1.8-fold), recovery rate (˃1.6-fold), and binding activity (˃2.6-fold increase in absorbance450nm). The produced scFv exhibited expected secondary structure as well as acceptable bio-functionality, homogeneity, and stability.Conclusion: We were able to produce ˃25 mg/L culture functional and stable anti-IL-1RAP scFv via recovering IBs by pulse dilution procedure. The produced scFv as a useful targeting agent could be used in scheming new therapeutics or diagnostics for myeloid malignancies.
Chemerin/CMKLR1 Axis Induces Proliferation and Invasion in Human Endometrial Cancer
Authors: Wei Ding , Yang Gao , Wei Zhang , Pengpeng Qu 
Published: June 2022

Abstract
Objectives: This study aimed to analyze the differences in the chemokine chemerin and its receptor chemokine-like receptor 1 (CMKLR1) between endometrial cancer (EC) patients and normal populations, and investigate the effect of CMKLR1 on the proliferation, migration and invasion of HEC-1B cells and its roles in Wnt/ β-catenin signaling pathways. Methods: The clinical data of 50 EC cases (study group) admitted from January 2018 to December 2020 were collected, and 50 cases of those who underwent a health checkup in Tianjin Central Hospital of Gynecology and Obstetrics during the same period were selected and included in the control group. The relationship between serum chemerin levels and clinicopathological features, as well as the difference in CMKLR1 protein expression between cancer and para-carcinoma tissue were compared. RT-PCR and Western blot methods were adopted to detect CMKLR1 expression in different EC cell lines and normal endometrial cells. CMKLR1 shRNA and NC shRNA stably transfected cell lines were constructed by shRNA interference technology to verify the interference efficiency. CCK-8 count assay, cell scratch test and transwell chamber were used to detect the effects of silencing CMKLR1 gene expression on the proliferation, migration and invasion of EC cells. Changes in Wnt/β-catenin signaling pathways were detected after silencing CMKLR1 gene expression by Western blot. Results:The ELISA showed that the serum chemerin level in EC group was higher than that in normal control group. In EC group, the serum chemerin level in advanced-stage patients was higher than that in early-stage patients (P < 0.05). Immunohistochemistry showed that the CMKLR1 expression in EC tissue was higher than that in paracarcinoma tissue. Cytological findings showed that CMKLR1 expression was the highest in HEC-1B compared with normal endometrial cells (NE) and EC cell lines HEC-1A and Ishikawa, and the silencing efficiency of CMKLR1 gene in HEC-1B cells treated by shRNA interference technology reached 70%. CCK-8 assay showed that silencing CMKLR1 gene expression can significantly reduce the proliferation rate of HEC-1B cells (P < 0.05). The scratch test showed that silencing CMKLR1 expression genes significantly inhibit the migration of HEC-1B cells (P < 0.05). Transwell chamber showed that silencing CMKLR1 gene expression can inhibit the invasion ability of HEC-1B cells (P < 0.05). Western blot detection showed that silencing CMKLR1 gene expression can significantly inhibit the protein expression of phosphorylated β-catenin and cyclin D1 protein in HEC-1B cells, and the difference was statistically significant (P < 0.05). Conclusion: The chemokine chemerin and its receptor CMKLR1 may be involved in the progression and metastasis of EC. Serum chemerin levels can be used as a molecular marker for early diagnosis of EC, and CMKLR1 gene can provide a new target for the potential gene therapy of EC.
Interpretable Predictive Model for Shield Attitude Control Performance Based on XGBoost and SHAP
Authors: Hu Min , Zhang Haolan , Wu Bingjian , Li Gang , Zhou Li 
Published: June 2022

Abstract
The sudden decline in the attitude control performance is a common abnormal situation during shield tunneling. When the problem happens, the shield driver will have difficulty controlling the shield's attitude, which will cause the shield to deviate from its design axis and affect the quality of the tunnel. The causes behind poor control performance are usually complicated, so how to choose appropriate countermeasures is a challenging problem. Based on the above issues, this paper proposes the Interpretable Predictive Model for Shield attitude Control Performance (IPM_SCP). The model first predicts the current shield control performance through the extreme gradient boosting (XGBoost) sub-model and then uses the Shapley additive explanation sub-model to interpret the model output. The model was tested on the left-line tunnel of the Hangzhou–Shaoxing railway project in the Ke-Feng section. The results reveal that the model could effectively predict the control performance of the shield and give the most influential parameter and the direction in adjusting the parameter to improve the shield's attitude control performance when the control performance decreases. Therefore, IPM_SCP gives the correct parameter adjustment instructions when the shield’s attitude control performance declines, and eventually improves tunnel construction quality and efficiency.
Choroidal Hemodynamics in Central Serous Chorioretinopathy after Half-dose Photodynamic Therapy and the Effects of Smoking
Authors: Horiguchi Etsuyo , Takeuchi Jun , Tomita Ryo , Asai Keiko , Nakano Yuyako , Ota Hikaru , Taki Yosuke , Ito Yasuki , Terasaki Hiroko , Nishiguchi Koji M , Kataoka Keiko 
Published: June 2022

Abstract
This retrospective study aimed to evaluate choroidal hemodynamics after half-dose photodynamic therapy (PDT) for chronic central serous chorioretinopathy (CSC) and the effects of smoking using laser speckle flowgraphy (LSFG). This study included 29 eyes of 29 patients treated with half-dose PDT for chronic CSC. The mean blur rate (MBR) in the PDT irradiation area (whole area), the pachyvessel (PV) area, non-PV (NPV) area, and filling delay (FD) area were assessed respectively. The MBR was also assessed by smoking status. The MBR significantly decreased in the whole, PV, NPV, and FD areas at all time points (P < 0.001). Of the 29 patients, 6 were never smokers, 13 were past smokers, and 10 were current smokers. At baseline, no significant difference was found in the MBR in the whole, PV, NPV, and FD areas among never, past, and current smokers. The MBR changes showed a significantly smaller decrease in current smokers than in never smokers in the whole (P = 0.021), PV (P = 0.009), and NPV (P = 0.034) areas, but not in the FD area (P = 0.172). Half-dose PDT for chronic CSC reduced choroidal blood flow in the PDT-irradiated area, which was blunted by current smoking status.
Use of plant growth regulators to reduce 2-methyl-4-chlorophenoxy acetic acid-Na (MPCA-Na) damage in cotton
Authors: Zhang Quancheng , Wang Jing , Wang Jun-Gang 
Published: June 2022

Abstract
Background2-methyl-4-chlorophenoxy acetic acid-Na (MPCA-Na) is a phenoxy carboxylic acid selective hormone herbicide that is widely used in crop fields. However, drift of MPCA-Na during application is highly damaging to cotton plants. This study was carried out from 2019 to 2020 to determine the effects of different concentrations of MPCA-Na on physiological metabolism and cotton yield at the seedling, budding, flowering and boll stages. Moreover, we evaluated the different combinations of brassinosteroids, gibberellin, phthalanilic acid and seaweed fertilizer, which used to ameliorate herbicide damage. ResultsMPCA-Na exposure caused a decrease in the chlorophyll content, and an increase in the soluble protein content, MDA content and protective enzyme activity. MPCA-Na also caused significant reductions in plant height, boll number and the single boll weight at the seedling and budding stages, but had little effect on plant height and the single boll weight at flowering and boll stages. Under the maximum recommended dose of MPCA-Na (130 g/L), the number of cotton bolls at seedling and bud stages decreased by 75.33% and 79.50%, respectively, and the single boll weight decreased by 46.42% and 36.31%, respectively. However, the number of cotton bolls and single boll weight at flowering and boll stages decreased by 48.15% and 5.38%, respectively. Application of plant growth regulators decreased the MDA content, and increasing chlorophyll, soluble protein content and protective enzyme activity, and reducing the degree of MCPA-Na damage. Positive effects were also observed in terms of cotton yield. ConclusionsThe results suggest that certain plant growth regulators could be used to alleviate MPCA-Na damage and maintain cotton yield. When the cotton exposed to MCPA-Na at the seedling stage should be treated with phthalanilic acid + seaweed fertilizer, while plants exposed at the budding stage should be treated with brassinosteroids + seaweed fertilizer, and those exposed at the flowering and boll stages should be treated with gibberellin + seaweed.
SparkGrid: Blockchain Assisted Secure Query Scheduling and Dynamic Risk Assessment for Live Migration of Services in Apache Spark based Grid Environment
Authors: G.M. Kiran , N Nalini 
Published: June 2022

Abstract
Grid computing is an emerging technology that enabled the heterogeneous collection of data and provisioning of services to the users. Due to the high amount of incoming heterogeneous request, grid computing needs an efficient scheduling to reduce execution time and satisfy Service Level Agreement (SLA) and Quality of Service (QoS) requirements. For that purpose, we proposed SprakGrid method to reduce execution time and satisfying SLA, QoS requirements. The proposed work includes four consecutive phases which are explained as follows, in first we perform user authentication in order to ensure the legitimacy of the users using Elliptic Curve based Chaos Theory (ECCT) algorithm which generate secret key and stored it into the blockchain. In second we perform query scheduling for resource discovery using Soft Actor Critic (SAC) algorithm by considering 3P’s parameters which is performed by spark environment that schedules optimal resources based on the service request. In third, we perform risk assessment and request dropping, in which the risk nodes of workers are evaluated by master node. To address the resource wastage by attacker, this research evaluates the risk value in a dynamic manner using Shannon entropy. Based on the risk assessment the requestsare classified into two classes such as normal and malicious. In fourth we perform service live migration, in which the malicious requests are dropped and normal request are migrated from source node to target node using Multi-Constraints based Emperor Penguin Optimization (MC-EPO). Finally, simulation is performed by GridSim and the simulation results demonstrate that the proposed SparkGrid method achieves superior performance compared to other state-of-the art methods.
Self-made fenestrated modified endograft for urgent an Aortic Arch Aneurysm: Case Report
Authors: Gallicchio Vito , Barbarisi Danilo , Condò Flavia , Sciarrillo Rosaria , Flora Loris 
Published: June 2022

Abstract
We report the case of an endovascular repair of an aortic arch aneurysm by a self-made endograft with a single fenestration in a high-risk patient unfit for open surgery. A patient of 81 years, chronic ischemic cardiopathic, suffering from prostate K in chemotherapy treatment, came to our hospital for post-traumatic fracture of the right femur, associated with dysphonia and respiratory disorders for several days. During the hospitalization, the patient performed angio-CT that found the presence of voluminous aneurysm of the aortic arch with a maximum diameter of about 70mm. The patient was treated in the first instance with a carotid-carotid-succlavium bypass to preserve the cerebral and upper limb vascularization. The self-made endograft was created by modifying a standard endograft by a single fenestration following the three-dimensional reconstructions of the CT images. The procedure was completed by implanting this endograft with stent delivery to the patient with a fenestration on the anonymous trunk. The procedure was successfully completed and postoperative course was uneventful. Computed tomography angiography demonstrated the exclusion of the aneurysm, patency of the implanted endograft modules, and absence of signs of endoleaks and / or cerebral or medullary ischemic complications.
Glycemic Response of Volunteers to the Consumption of Supplements and Food Formulas for Oral and/or Enteral Nutrition
Authors: Giuntini Eliana Bistriche , Ruffo Giovanna A , de Abreu Camila LM , Russo Marina C , Vidal Jady A A , Rocha Mariana V 
Published: June 2022

Abstract
PurposeThe objective of this study was to determine the glycemic index (GI) and glycemic load (GL) of three products from the Brazilian market used as supplement and food formula for oral and/or enteral nutrition.MethodsThe volunteers (n=16) attended Food Research Center weekly for six weeks, after a 10 h to 12 h overnight fasting, where their glycemic response was evaluated for 120 min, resulting from the consumption of glucose solution (reference food, three times) and three products: Cubitan® vanilla, Diasip® chocolate and Diasip® vanilla. GI was determined by calculating the area under the glycemic response curve using the trapezoidal rule and ignoring the areas below the fasting line and considering GI of glucose to be 100. To determine GL it was considered the amount of carbohydrates available in a standard serving of the product and GI.ResultsThe three products studied showed low GI and low GL (Cubitan® GI=35, GL=6; Diasip® chocolate GI=49, GL=7; Diasip® vanilla GI=47, GL=7), with significant differences from those and the reference food, but no significant difference between them. Similar results were also observed for the blood glucose peak, which occurred 30 min after the consumption of all products.ConclusionsGI and GL of the products were considerably lower than those of the reference food. The products evaluated presented a low glycemic response, showed by a glycemic response curve with a slightly accentuated shape and no high peaks.
Translocation of a single Arg9 peptide across a DOPC/DOPG(4:1) model membrane using the weighted ensemble method
Authors: Choe Seungho 
Published: June 2022

Abstract
A spontaneous translocation of cell-penetrating peptides(CPPs) in all-atom molecular dynamics(MD) simulations is not expected within a short time scale (e.g., a few hundred ns) because
it is believed that the time required for the translocation of usual CPPs is on the order of minutes. In this study, we report a single Arg9(R9) peptide translocation across a DOPC/DOPG(4:1) model membrane within an order of a few tens ns scale by using the weighted ensemble(WE) method, one of the most powerful and flexible path sampling techniques. We present free energy profiles of the translocation across the membrane and identify how water molecules play a role during the translocation. We also show how the orientation of Arg9 affects the translocation and how lipid molecules were transported along with Arg9. Our approach can help study interactions of CPPs with various model membranes within MD simulation approaches.
Determination of some heavy metal and mineral contents microbiological and chemical properties of Çökelek cheeses
Authors: Kalaycı Nagihan , Ürkek Bayram , Öztürk Feyza , Şengül Mustafa , Çiftçi Esengül 
Published: June 2022

Abstract
The Çökelek samples what 30 different, were collected from randomly local bazaars to investigate heavy metal contaminant and mineral levels and some physicochemical and microbiological properties of samples. The As was detected in the 4 of 30 samples, while the Pb was determined in the 6 of 30 samples. The mean major and trace element contents of Çökelek samples were ordered as Na > P > Ca > K > Mg and Al > Zn > Ni > Cu, respectively. The physicochemical properties indicated a high deviation among samples. The mean total solids, ash, salt, fat, protein waters soluble nitrogen contents and ripening index of samples were 29.83%, 1.88%, 0.68%, 4.31%, 19.84%, 0.33%, and 1.79%, respectively. The mean TAMB count of Çökelek samples were found as 8.26 log CFU g− 1. The coliform bacteria and yeast-mold counts were detected in 11 and 27 of 30 samples, respectively. The mean coliform and yeast-mold counts were 1.82 log CFU g− 1 and 7.11 log CFU g− 1, respectively. There are not mentioned traditional cheeses in the legal rules such as the Turkish Food Codex. So, there is no legal limit and standard production processes. This situation is a problem in terms of traditional cheese quality. For this reason, traditional cheese should perform a further studied, and determine the legal limits.