bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Modified Systemic Lupus Erythematosus Responder Index Attainment With Long‐Term Clinical Outcomes: A Five‐Year Prospective Study
Authors: Connelly Kathryn , Kandane‐Rathnayake Rangi , Hoi Alberta , Louthrenoo Worawit , Hamijoyo Laniyati , Luo Shue Fen , Wu Yeong‐Jian Jan , Cho Jiacai , Lateef Aisha , Lau CS , Chen Yi‐Hsing , Navarra Sandra , Zamora Leonid , Li Zhanguo , An Yuan , Sockalingam Sargunan , Hao Yanjie , Zhang Zhuoli , Chan Madelynn , Katsumata Yasuhiro , Harigai Masayoshi , Oon Shereen , Bae Sang‐Cheol , O'Neill Sean , Gibson Kathryn A. , Basnayake BMDB , Kikuchi Jun , Takeuchi Tsutomu , Ng Kristine Pek Ling , Tugnet Nicola , Kumar Sunil , Goldblatt Fiona , Law Annie , Tee Michael , Tee Cherica , Tanaka Yoshiya , Ohkubo Naoaki , Tan Jin Yu , Karyekar Chetan S. , Nikpour Mandana , Golder Vera , Morand Eric F. 
Published: September 2022

ObjectiveIn trials of systemic lupus erythematosus (SLE), the SLE Responder Index (SRI) is the most commonly used primary efficacy end point but has limited validation against long‐term outcomes. We aimed to investigate associations of attainment of a modified version of the SRI (mSRI) with key clinical outcomes in SLE patients with up to 5 years of follow‐up.MethodsWe used data from a large multicenter, longitudinal SLE cohort in which patients received standard of care. The first visit with active disease (defined as SLE Disease Activity Index 2000 [SLEDAI‐2K] score ≥6) was designated as baseline, and mSRI attainment (defined as a reduction in SLEDAI‐2K ≥4 points with no worsening in physician global assessment ≥0.3 points) was determined at annual intervals from baseline up to 5 years. Associations between mSRI attainment and outcomes including disease activity, glucocorticoid dose, flare, damage accrual, Lupus Low Disease Activity State (LLDAS), and remission were studied.ResultsWe included 2,060 patients, with a median baseline SLEDAI‐2K score of 8. An mSRI response was attained by 56% of patients at 1 year, with similar responder rates seen at subsequent annual time points. Compared to nonresponders, mSRI responders had significantly lower disease activity and prednisolone dose and higher proportions of LLDAS and remission attainment at each year, and less damage accrual at years 2 and 3. Furthermore, mSRI responder status at 1 year predicted clinical benefit at subsequent years across most outcomes, including damage accrual (odds ratio [OR] range 0.58–0.69, P < 0.05 for damage accrual ORs at all time points).ConclusionIn SLE patients with active disease receiving standard of care, mSRI attainment predicts favorable outcomes over long‐term follow‐up, supporting the clinical meaningfulness of SRI attainment as an SLE trial end point.
Multistate modeling and structure selection for multitype recurrent events and terminal event data
Authors: Ma Chuoxin , Wang Chunyu , Pan Jianxin 
Published: September 2022

AbstractIn cardiovascular disease studies, a large number of risk factors are measured but it often remains unknown whether all of them are relevant variables and whether the impact of these variables is changing with time or remains constant. In addition, more than one kind of cardiovascular disease events can be observed in the same patient and events of different types are possibly correlated. It is expected that different kinds of events are associated with different covariates and the forms of covariate effects also vary between event types. To tackle these problems, we proposed a multistate modeling framework for the joint analysis of multitype recurrent events and terminal event. Model structure selection is performed to identify covariates with time‐varying coefficients, time‐independent coefficients, and null effects. This helps in understanding the disease process as it can detect relevant covariates and identify the temporal dynamics of the covariate effects. It also provides a more parsimonious model to achieve better risk prediction. The performance of the proposed model and selection method is evaluated in numerical studies and illustrated on a real dataset from the Atherosclerosis Risk in Communities study.
Machine learning approaches to root cause analysis, characterization, and monitoring of subvisible particles in monoclonal antibody formulations
Authors: Greenblott David N. , Zhang Jingtao , Calderon Christopher P. , Randolph Theodore W. 
Published: September 2022

AbstractProcessing stresses on therapeutic proteins may cause formation of subvisible particles. Different stress mechanisms generate particle populations with characteristic morphological “fingerprints,” and machine learning techniques like convolutional neural networks (CNNs) allow classification of microscopy images of these particles according to known stresses at their root cause. Using CNNs to classify novel particle types not included during network training may lead to inaccurate classification, however, using CNNs to monitor the presence of particulate matter not explicitly used in training could serve as a useful process analytical technology. We used CNNs to classify and identify the root cause of particles generated by subjecting three monoclonal antibodies (mAbs) to various common manufacturing stresses. We probed the generality of particles generated by stressing different mAbs in different formulations and showed that CNN analyses were sensitive not only to the applied stress, but also the buffer conditions and the particular mAb that generated particle populations. Thus, models trained on images of particles created with one mAb and buffer system may not provide accurate root cause analysis when applied to particles generated by other mAb and buffer systems. A lever‐rule analysis of CNN‐derived fingerprints was used to characterize the composition of mixtures of particle types. Finally, we monitored the temporal evolution of CNN‐derived fingerprints when novel populations of particles, which were not included during training, were generated by pumping mAb solutions through a peristaltic pump.
Vaspin accelerates the proliferation, invasion and metastasis of Triple‐Negative breast cancer through MiR‐33a‐5p/ABHD2
Authors: Cao Xin‐Hui , Chen Xiu , Yang Kai , Wang Ya‐Lin , Liang Ming‐Xing , Fei Yin‐Jiao , Tang Jin‐Hai 
Published: September 2022

AbstractObjectiveTo explore the influence and the underlying mechanism of vaspin (visceral adipose tissue‐derived serpin) on the development of triple‐negative breast malignancy.MethodsFirst, we analyzed medical records and screened out 22 breast cancer patients with different BMI according to inclusion and exclusion criterion, and measured serum vaspin of those patients. Then we studied the effects of vaspin on TNBC cell lines by using EdU assay, colony formation, transwell and wound‐healing assay. Later, we used bioinformatics analysis to identify downstream effectors and verify with qRT‐PCR, luciferase assay, western blot, etc.ResultsWe found the vaspin level was positively correlated with BMI in breast malignant patients and vaspin could significantly enhance the proliferation, infiltration and transferring of triple‐negative breast cancer cells by restraining the expression of miR‐33a‐5p. By using bioinformatic analysis and luciferase assay, we identified miR‐33a‐5p directly regulating ABHD2.ConclusionVaspin, as a cancer‐promoting cytokine, may inhibit miR‐33a‐5p thus increasing the level of ABHD2 to promote the development of the triple‐negative breast cancer.
Semi‐Quantitative Discrimination of Honey Adulterated with Cane Sugar Solution by an ETongue
Authors: Dias Luís G. , Bruni Andressa R. S. , Anizelli Camilla P. , Zangirolami Marcela , Lima Patrícia C. , Estevinho Leticia M. , Bona Evandro 
Published: September 2022

AbstractThis study successfully applied a potentiometric E‐tongue with 20 cross‐selectivity lipidic polymeric membranes in the discrimination of three semi‐quantitative groups, that represented the following intervals of honey adulteration percentage with cane sugar: 0 %; [0, 10]%; [10, 20]% of adulteration. We analysed five different types of Portuguese honey; five brands of cane sugar were added to the adulterated samples; a comparative analysis was then performed. Linear discriminant analysis coupled with a tabu search algorithm for feature selection was applied to the ETongue's analytical data to select the best model. A discriminant model with 12 sensors was obtained. This model classified correctly all samples in both in internal (train data, 15 samples) and external validation (test data,10 samples). Also, multiple linear regression with tabu search was applied to verify if ETongue's data would allow quantifying the honey's adulteration level. The results showed that it was possible to obtain a quantitative model but with unsatisfactory predictive performance in the test data group (external validation), giving, in general, values below the expected concentrations. E‐tongue is a real‐time green, flexible and low‐cost analytical tool that requires minimum sample preparation and no special technical skills, being a promising tool for everyday application.
Massive hematochezia due to jejunal varices successfully treated with coil embolization
Authors: Nasr Sahar , Dahmani Wafa , Jaziri Hanene , Becheikh Yesser , Ameur Wafa Ben , Elleuch Nour , Jmaa Ali 
Published: September 2022

AbstractWe report a case of hematochezia with hemodynamic instability due to jejunal varices in a cirrhotic patient with no prior history of surgery. The patient was managed with coil embolization via the portal vein. After which, the patient did not present any hemorrhage recurrence and maintained a stable hemoglobin level.
Adsorptive Removal of Daunorubicin from Water by Graphene Oxide, Activated Carbon, and Multiwalled Carbon Nanotubes: Equilibrium and Kinetic Studies
Authors: Ghodrati Atefeh , Shahrouzi Javad Rahbar , Nemati Ramin , Pourjafari Neda 
Published: September 2022

AbstractThe adsorption of anticancer drug daunorubicin from aqueous solution by carbonaceous adsorbents was investigated. The adsorption performances of graphene oxide (GO), activated carbon (AC), and multiwalled carbon nanotubes (MWCNTs) were compared. The effects of solution pH, contact time, and initial daunorubicin concentration on the removal of daunorubicin were explored. Isotherm and kinetic models were applied to predict the efficiency of the adsorbents. The major functional groups of the adsorbents were identified by FTIR spectroscopy. In alkaline medium, adsorption increases with increasing pH value. With increasing initial concentration, adsorption capacity increases and removal efficiency decreases. Kinetic studies showed that the pseudo‐second‐order model describes adsorption onto GO well, whereas the intraparticle diffusion model gives the best fits for AC and MWCNTs. The adsorption isotherm fits the Langmuir model very well.
STED analysis reveals the organization of nonmuscle muscle II, muscle myosin II, and F‐actin in nascent myofibrils
Authors: Wang Jushuo , Fan Yingli , Sanger Jean M. , Sanger Joseph W. 
Published: September 2022

AbstractA three‐step model has been proposed to describe myofibril assembly in vertebrate cardiac and skeletal muscle cells beginning with premyofibrils, followed by nascent myofibrils, and ending as mature myofibrils (reviewed in Sanger, Wang, et al. (2017). Assembly and maintenance of myofibrils in striated muscle. Handbook of Experimental Pharmacology 235, 39–75; Wang, Fan, (2020). Myofibril assembly and the roles of the ubiquitin proteasome system. Cytoskeleton 77, 456–479). Premyofibrils are composed of minisarcomeres that contain nonmuscle myosin II filaments interdigitating with actin filaments embedded at their barbed ends in muscle‐specific alpha‐actinin‐rich Z‐bodies. Sarcomeres in mature myofibrils have filaments of muscle myosin II that interact with actin filaments that are attached to muscle alpha‐actinin in Z‐bands. Nascent myofibrils, the transitional step between premyofibrils and mature myofibrils, possess two types of myosins II, that is, nonmuscle myosin II and muscle myosin II. The relationship of these two different myosins II in nascent myofibrils, however, is not clear. Stimulated emission depletion (STED) microscopic analyses of nascent myofibrils in both embryonic chick cardiomyocytes, and hiPSC‐derived cardiomyocytes revealed that nonmuscle myosin II is in the middle of the nascent myofibril, surrounded by overlapping muscle myosin II filaments at the periphery, and non‐striated filamentous actin is present in the nascent myofibril. These findings support the original three‐step model of myofibril assembly proposed by Rhee, Sanger, and Sanger, (1994). The premyofibrils: Evidence for its role in myofibrillogenesis. Cell Motility and the Cytoskeleton 28, 1–24.
Cancer risk associated with cytomegalovirus infection among solid organ transplant recipients in the United States
Authors: Geris Jennifer M. , Spector Logan G. , Pfeiffer Ruth M. , Limaye Ajit P. , Yu Kelly J. , Engels Eric A. 
Published: September 2022

BackgroundCytomegalovirus (CMV) is among the most common viral infections after solid organ transplantation (SOT). Associations of CMV with cancer risk among SOT recipients have been incompletely evaluated.MethodsThe authors used linked data from the US SOT registry and 32 cancer registries. Poisson regression was used to compare cancer incidence across CMV risk groups based on donor (D) and recipient (R) immunoglobulin G (IgG) serostatus: high risk (R‐negative/D‐positive), moderate risk (R‐positive), and low risk (R‐negative/D‐negative).ResultsIn total, 247,318 SOT recipients were evaluated during 2000–2017 (R‐negative/D‐positive, 20.3%; R‐positive, 62.9%; R‐negative/D‐negative, 16.8%). CMV‐seropositive recipients were older, more racially/ethnically diverse, and had lower socioeconomic status than CMV‐seronegative recipients. Compared with R‐negative/D‐negative recipients, recipients in the R‐negative/D‐positive and R‐positive groups had a lower incidence of diffuse large B‐cell lymphoma (DLBCL; R‐negative/D‐positive: adjusted incidence rate ratio [aIRR], 0.74; 95% confidence interval [CI], 0.59–0.91; R‐positive: aIRR, 0.83; 95% CI, 0.69–1.00). CMV serostatus modified the association between Epstein–Barr virus (EBV) status and DLBCL (p = .0006): DLBCL incidence was increased for EBV R‐negative/D‐positive recipients (aIRR, 3.46; 95% CI, 1.50–7.95) among CMV R‐negative/D‐negative recipients but not among the other CMV risk groups. Compared with recipients who were CMV R‐negative/D‐negative, those who were R‐negative/D‐positive had a lower incidence of small intestine cancer (aIRR, 0.23; 95% CI, 0.09–0.63), and R‐positive recipients had a higher incidence of lung cancer (aIRR, 1.24; 95% CI, 1.05–1.46). CMV status was not associated with risk for other cancers.ConclusionsCMV status was not associated with risk for most cancers among SOT recipients. The inverse association with DLBCL may reflect the protective effects of CMV prophylaxis or treatment with off‐target efficacy against EBV infection (the major cause of lymphoma in SOT recipients).
Binary Mixtures in Linear Convection Arrays
Authors: Ghosh Pulak K. , Zhou Yuxin , Li Yunyun , Marchesoni Fabio , Nori Franco 
Published: September 2022

AbstractWe numerically investigated the dynamics of a mixture of finite‐size active and passive disks in a linear array of two‐dimensional convection rolls. The interplay of advection and steric interactions produces a number of interesting effects, like the stirring of a passive colloidal fluid by a small fraction of slow active particles, or the separation of the mixture active and passive colloidal fractions by increasing the motility of the active one, which eventually clusters in stagnation areas along the array walls. These mechanisms are quantitatively characterized by studying the dependence of the diffusion constants of the active and passive particles on the parameters of the active mixture fraction.
Discovery of pyrazole‐1‐carboxamide derivatives as novel Gi‐biased μ‐opioid receptor agonists
Authors: Jung Jae‐Hoon , Jang In Hee , Kim Yeo Ok , Kim Sunhong , Yoon Myung Ha , Kim Yong‐Chul 
Published: September 2022

Abstractμ‐Opioid receptor (MOR) Gi‐biased agonists with no recruitment of β‐arrestin were introduced as a new analgesic strategy to overcome the conventional undesirable side effects of opioid receptor‐targeted drugs, such as tolerance, addiction, respiratory depression, and constipation. For the development of novel Gi‐biased MOR agonists, the design, synthesis, and structure–activity relationship (SAR) analysis of the aminopyrazole core skeleton were conducted according to the current SAR data of PZM21 (2a) and its derivatives. New derivatives were biologically evaluated for their agonistic effects on cyclic adenosine monophosphate (cAMP) levels for the Gi pathway and β‐arrestin recruitment in MOR/κ‐opioid receptor/δ opioid receptor. An optimized selective Gi‐biased agonist, Compound 17a, was discovered with potent cAMP inhibitory activities, with a 50% efficacy concentration value of 87.1 nM and no activity in the MOR β‐arrestin pathway and other subtypes. The in vivo pain relief efficacy of Compound 17a was confirmed in a dose‐dependent manner with spinal nerve ligation and cisplatin‐induced peripheral neuropathy rodent neuropathic pain models.
Estimating the population size of a mountain galliform in the context of multi‐stakeholder adaptive management
Authors: Calenge Clément , Ménoni Emmanuel , Milhau Blandine , Foulché Kévin , Chiffard Jules , Marchandeau Stéphane 
Published: September 2022

AbstractWe designed a participatory monitoring program for the capercaillie population in the French Pyrenees based on lek censuses conducted during the breeding season. This program was implemented by a consortium of stakeholders interested in the conservation of French galliforms. The program, carried out since 2010, relied on a dual frame sampling approach: The first sampled frame was the list of all known leks in the study area. We distinguished two types of known leks: leks known to be active before the onset of the program (with at least one cock detected since 2000) and leks with an indeterminate activity status at the time of the onset of the program. The monitoring program also accounted for the existence of leks that were unknown due mainly to incomplete expert knowledge. We therefore built a complementary area frame by discretizing the study area into a set of 4‐km2 grid cells. These cells were then sampled and searched to find unknown leks. When unknown leks were found, cock censuses were organized. An additional field experiment allowed us to estimate the detection probability of unknown leks during these cell searches. We then fitted two hierarchical models: (i) An N‐mixture model fitted to the lek census data set allowed us to estimate the mean number of cocks on the three types of leks (known active, known indeterminate, and unknown leks); and (ii) another model fitted to the cell search data set allowed us to estimate the number of unknown leks in the studied mountain range. By multiplying the estimated mean numbers of cocks associated with the three types of leks by the number of leks of each type (an estimated value in the case of unknown leks), we obtained estimates of the total numbers of cocks on all leks at different spatial scales in the study area every 2 years. Our model suggests that the capercaillie cock population was stable from 2010 to 2017 over the whole range but decreased slightly in the foothill area and western part, a decrease that worsened in 2018–2019.
The COVID‐19 pandemic and youth with anorexia nervosa: A retrospective comparative cohort design
Authors: Datta Nandini , Van Wye Eliza , Citron Kyra , Matheson Brittany , Lock James D. 
Published: September 2022

AbstractObjectiveThere is a growing body of literature suggesting the novel coronavirus pandemic (COVID‐19) negatively impacts mental health in individuals self‐reporting an eating disorder (ED); however, limited pediatric data is available about the impact COVID‐19 has had on youth with EDs, specifically Anorexia Nervosa (AN). Our study uses a cross‐sectional design to explore differences in ED symptoms between adolescents diagnosed with AN during the COVID‐19 pandemic compared to a retrospective cohort of adolescents for whom these measures were previously collected, prior to the pandemic.MethodWe report cross‐sectional data assessing differences between AN behaviors and cognitions during the COVID‐19 pandemic compared to a retrospective cohort (n = 25 per cohort) assessed before the pandemic.ResultsResults suggest that individuals with a first‐time diagnosis of AN during the pandemic had lower percent expected body weight, and more compulsive exercise behaviors.ConclusionsThese data support existing pediatric findings in exercise and body weight differences in adolescents with AN before and during the pandemic. Findings may be helpful in informing considerations for providers treating ED patients amidst and after the pandemic.Public SignificanceThis manuscript compares a retrospective cohort of adolescents diagnosed with AN prior to the pandemic to a cohort of adolescents diagnosed with AN during the pandemic. Results report that adolescents diagnosed with AN during the pandemic have lower weights and increased compensatory exercise behavior compared to adolescents diagnosed with AN before the pandemic despite no difference in length of illness. Findings may be helpful in informing considerations for providers treating ED patients amidst and after the pandemic.
Is hunting nonintentionally selective? A test using game bird capture‐dead recoveries
Authors: Grzegorczyk Emilienne , Bézier Léa , Le‐Rest Kévin , Caizergues Alain , Francesiaz Charlotte , Champagnon Jocelyn , Guillemain Matthieu , Eraud Cyril 
Published: September 2022

AbstractSelective hunting has various impacts that need to be considered for the conservation and management of harvested populations. The consequences of selective harvest have mostly been studied in trophy hunting and fishing, where selection of specific phenotypes is intentional. Recent studies, however, show that selection can also occur unintentionally. With at least 52 million birds harvested each year in Europe, it is particularly relevant to evaluate the selectivity of hunting on this taxon. Here, we considered 211,806 individuals belonging to 7 hunted bird species to study unintentional selectivity in harvest. Using linear mixed models, we compared morphological traits (mass, wing, and tarsus size) and body condition at the time of banding between birds that were subsequently recovered from hunting during the same year as their banding, and birds that were not recovered. We did not find any patterns showing systematic differences between recovery categories, among our model species, for the traits we studied. Moreover, when a difference existed between recovery categories, it was so small that its biological relevance can be challenged. Hunting of birds in Europe therefore does not show any form of strong selectivity on the morphological and physiological traits that we studied and should hence not lead to any change of these traits either by plastic or by evolutionary response.