bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple datasets to explore the molecular mechanism of sepsis
Authors: Lin Shuang , Luo Bin , Ma Junqi 
Published: April 2022

Abstract
Background: This study aimed to identify potential biomarkers, by means of bioinformatics, affecting the occurrence and development of septic shock.Methods: The self-test data and differential genes in GSE131761 were screened, and the diagnostic model of sepsis was further constructed by Lasso regression to identify key genes. The stability of the model was verified by ROC curve. Finally, immune infiltration analysis, enrichment analysis, transcriptional regulation analysis and correlation analysis were performed.Results: A total of 292 differential genes were screened out from the self-test data, 294 differential genes were screened out by GSE131761, Lasso regression was performed on the intersection genes of the two, a diagnostic model was constructed, and 5 genes were identified as biomarkers of septic shock. These 5 genes were SIGLEC10, VSTM1, GYPB, OPTN, and GIMAP7. The five key genes were strongly correlated with immune cells, and the ROC results showed that the five genes had good predictive performance on the occurrence and development of diseases. In addition, the key genes were strongly correlated with immune regulatory genes.Conclusion: In this study, a series of algorithms were used to identify five key genes that are associated with septic shock, which may become potential candidate targets for septic shock diagnosis and treatment.Trial registration：Approval number：2019XE0149-1，Project name：Study on the relationship between TLRs signaling pathway and sepsis.
Association of hemoglobin levels with bone mineral density in the thoracic and lumbar spine for adults over 18 years of age: a cross-sectional study
Authors: Liu En Qi , Hou Xin Zheng , Liu Si Qi , Han Jing , Lv Hao 
Published: April 2022

Abstract
Aim:The overall object of this study is to find out the association between hemoglobin (HGB) and bone mineral density (BMD) in the lumbar and thoracic spine among adults over the age of 18.Methods:The current cross-sectional study used the non-institutionalised US population from the National Health and Nutrition Survey (NHANES) as the sample source. A multiple linear regression model and smoothed curve fitting were used to show the assessment of the relationship between hemoglobin and bone mineral density in the lumbar and thoracic spine, with analysis of subgroups according to gender and race.Results:After accounting for relevant confounders, HGB was negatively associated with bone mineral density in multiple regression models. In subgroup analyses stratified by sex or race, however, the relationship between HGB and thoracic spine BMD was only found in women and Mexican-Americans, and the association with lumbar spine BMD was present in males and females, Mexican-American and Non-Hispanic White. Among participants in other races and those whose race was not reported, the relationship between HGB with BMD in the lumbar spine and the thoracic spine showed an inverted U-shaped curve (turning point: HGB 11.9 g/dL).Conclusion: HGB was negatively associated with BMD in the lumbar spine and BMD in the thoracic spine. However, this significant correlation varies by gender and race.
Study of the Intraocular Pressure Changes after Phacoemulsification in Patients with Refractive Errors
Authors: Zeid Mona M. , Abdel Tarek H. , Helaly Hany H. , Said Amr 
Published: April 2022

Abstract
Purpose: to investigate the intraocular pressure (IOP) changes after phacoemulsification surgery, and its relationship with refractive conditions. Materials and Methods: This prospective, observational study comprised 100 eyes from 100 patients who had no glaucoma and underwent phacoemulsification and intraocular lens implantation in one eye. Patients were classified into four groups by refractive conditions: Emmetropia, mild to moderate myopia, high myopia and hyperopia. Basic information was collected including age, sex, anterior chamber depth and axial length before surgery. IOP and refractive conditions measured before surgery, and at 1st day, 1st week, 1st month, and 3rd months after surgery. Results: all group showed a significant lower IOP at 3rd month compared with that before surgery. patients with emmetropia, mild to moderate myopia and hyperopia showed exactly the same results. In these 3 groups, IOP after surgery presented with a statistically significant drop at 1st week, 1st month and 3rd months after operation, especially at 1week. In patients with high myopia, IOP showed a lower IOP at 1st week and 1st month compared with IOP before surgery but this drop was not statistically significant (P= 0.505 and P = 0.146, respectively) but there is a statistically significant reduction between preoperative IOP and postoperative IOP at 3rd months (P =0.001). Conclusions: a significant lower IOP at 3rd month than that before surgery was found in patients groups with different refractive conditions. This indicate that cataract surgery had an effect on IOP reduction despite the refractive conditions. However, high myopic patients showed a non-significant lower speed of IOP reduction at 1st week and 1st month. This conclude that refractive conditions might influence the IOP fluctuation in first 90 days after surgery.
Serum diamine oxidase activity derived from responce to chemotherapy affects adverse events and serum amino acid levels
Authors: Sato Yuta , Tanaka Yoshihiro , Imai Takeharu , Okumura Naoki , Matsuhashi Nobuhisa , Takahashi Takao , Shimokawa Toshio , Yoshida Kazuhiro 
Published: April 2022

Abstract
Purpose The relation between activity of the small intestinal villi and the effectiveness of chemotherapy remains unclear. This study aimed to investigate how serum diamine oxidase (DAO) activity affects antitumor effects, adverse events, and amino acid absorption.Methods We performed a single-center prospective cohort study that enrolled 50 patients with esophageal cancer (EC) receiving docetaxel, cisplatin, and 5-fluorouracil therapy. We determined the cut-off value of serum DAO activity contributing to a response to chemotherapy using a generalized additive model. Additionally, we compared adverse events, inflammatory markers, blood amino acid levels, and quality of life between the high and low DAO activity groups during chemotherapy.Results The cut-off value of serum DAO activity at the first visit that contributed to a chemotherapy response was 6.5 units/L. Leukopenia and neutropenia of Grade ≥3 were significantly higher in the DAO low (<6.5 units/L) group (p = 0.044, 0.017, respectively). Interleukin-6 was significantly lower in the DAO high (≥6.5 units/L) group at the first visit and at 4 weeks after the end of chemotherapy (p = 0.039, 0.011, respectively). Glutamine was higher in the DAO high group at all measurement points during chemotherapy. Fatigue was significantly lower in the DAO high group (p = 0.001).Conclusion Serum DAO activity may be a predictor of the response to chemotherapy in patients with EC. The absorption capacity of amino acids was maintained in the group with high DAO activity, which may have contributed to the anti-inflammatory effect and provided a background for reducing adverse events.
Influences of a Novel Cylindrical Solar Dryer on Farmer’s Income and its Impact on Environment
Authors: Sharma Kirtika , Kothari Surendra , Panwar NL , Patel Maga Ram 
Published: April 2022

Abstract
Poor grain drying facilities along with the burden of drying cost bound the farmers to market their produce soon after harvest. Thus, this research paper intends to study the influences of a novel cylindrical solar assisted dryer on farmer’s income and its impact on environment. The paper also presents design and fabrication of a drying system for maize cobs by the use of non-conventional solar energy. Performance of the solar assisted drying system was also investigated for drying of yellow dent maize cobs. Drying, as a very energy-intensive post-harvest process, consumes a lot of electricity, which is usually provided by conventional energy. Here, solar dryers are the perfect solution in terms of efficiency, uniform drying of agricultural food products, less drying time, increased marketability of agricultural products as well as reducing load on farmer’s pocket for drying. With a high internal rate of return of 66 percent, the designed dryer proved to be technically and economically viable. When compared to open sun drying, the solar drying system produced better results in terms of quality and drying time. When compared to other models, the Middili model fits the experimental maize drying data better, with a coefficient of determination R2= 0.89729. Energy analysis inferred savings of 1352.97 kWh electrical energy and 128.18 liter of diesel fuel plus a reduction of 1.22 t CO2 per annum can be achieved by using this dryer.
Assessment of the Effects of Dexmedetomidine on Outcomes of Traumatic Brain Injury Using Propensity Score Analysis
Authors: Xu Jinbu , Xiao Qing 
Published: April 2022

Abstract
Background: Dexmedetomidine has been found to be protective against traumatic brain injury(TBI) in animal studies and safe to for use in previous clinical studies, but whether it improves TBI patient survival remains to be determined. We sought to answer this question through the analysis of data from the MIMIC clinical database.Methods: Data from TBI patients from the MIMICⅢ and MIMICⅣ databases were extracted and divided into a dexmedetomidine group and a control group. In the former group, dexmedetomidine was used for sedation, while in the latter, it was not used. Parameters including patient age, sex, the Acute Physiology score Ⅲ, the Charlson Comorbidity Index score, the Glasgow Coma Scale, other sedatives used, and pupillary response within 24h were employed in propensity score matching to achieve a balance between groups for further analysis. The length of hospital stay and outcome upon discharge were used to perform survival analysis. Subgroup analysis was performed to consolidate the results. Results: Different types of survival analyses demonstrated the same trend. Dexmedetomidine significantly improved TBI patient survival in the univariate analysis, multivariate analysis, analysis based on propensity score matching, and analysis based on the inverse probability of treatment weighting. Different doses, durations, and start time did not alter the beneficial efficacy of dexmedetomidine.Conclusions: Dexmedetomidine can be used to improve the outcomes of TBI patients.
Active monitoring of live virus: A Tale of Two Experimental Infectious Disease Vaccines
Authors: McCracken Reilly , Davide Joseph , Salovich Daniel , Whitmer Travis , Bhat Aditya , Vlasak Josef , Ha Sha , Yi Sijia , Mukherjee Malini , Sehlin Darrell , Ploeger Kristin , Califano Joseph 
Published: April 2022

Abstract
In the development of end-to-end large-scale live virus vaccine (LVV) manufacturing, process analytical technology (PAT) tools enable timely monitoring of critical process parameters (CPP) and significantly guide process development and characterization. In a commercial setting, these very same tools can enable real time monitoring of CPPs on the shop floor and inform harvest decisions, predict peak potency, and serve as surrogates for release potency assays. Here we introduce the development of four advanced PAT tools for upstream and downstream process monitoring in LVV manufacturing. The first tool explores the application of capacitance probes for real time monitoring of viable cell density in bioreactors. The second tool utilizes high content imaging to determine optimum time of infection in a microcarrier process. The third tool uses flow virometry (or nanoscale flow cytometry) to monitor total virus particle counts across upstream and downstream process steps and establishes a robust correlation to virus potency. The fourth and final tool explores the use of nucleic acid dye staining to discriminate between “good” and “damaged” virus particles and uses this strategy to also monitor virus aggregates generated sometimes during downstream processing. Collectively, these tools provide a comprehensive monitoring toolbox and represent a significantly enhanced control strategy for the manufacturing of LVVs.
SIGIRR Deficiency Contributes to CD4 T Cell Abnormalities by Facilitating the IL1/C/EBPβ/TNF-α Signaling Axis in Rheumatoid Arthritis
Authors: TENG Xiu , Mou Da-Chao , Tang Meng , Li Hui-Fang , Wu Sha-Sha , Jiao Ling , Pi Jin-kui , Wang Yan , Zhu Meng-Li 
Published: April 2022

Abstract
Rheumatoid arthritis (RA) is a complex autoimmune disease with multiple etiological factors, among which aberrant T-cell activation plays a key role in the initiation and perpetuation of the disease. The factors that trigger T cell abnormalities in these inflammatory processes are incompletely understood. SIGIRR (single immunoglobulin IL-1R-related receptor) is a member of the IL-1 receptor (ILR) family with distinct structural and functional characteristics, acting as a negative regulator of ILR and Toll-like receptor (TLR) downstream signaling pathways and inflammation. Here, we identified defects in SIGIRR in activated CD4 T cells as an underlying dysregulation. SIGIRR was preferentially distributed by human memory CD4 T cells, as revealed by single-cell RNA sequencing, and its expression was substantially reduced in RA patient-derived memory CD4 T cells, which was inversely associated with RA disease activity. SIGIRR-deficient mice were more susceptible to antigen-induced arthritis (AIA) attributed to unleashed tumor necrosis factor (TNF)-α production in antigen-specific CD4 T cells. Mechanistically, SIGIRR regulates the IL-1/C/EBPβ/TNF-α signaling axis, as established by experimental evidence and cis-acting factor bioinformatics analysis. Taken together, SIGIRR deficiency in RA CD4 T cells raised the possibility that receptor interference can target key abnormalities in T cells and represents a potentially novel strategy for immunomodulatory therapy.
Drainage volume on postoperative day one to predict clinically relevant postoperative pancreatic fistula following distal pancreatectomy
Authors: Zhou Quanyu , he Wei , liu Yao , liao Bo , Liang Yong , bing Mo , Yin Shujun , Tang Weian , Shi Yuhong , Xia Yuxiao 
Published: April 2022

Abstract
Background: The purpose of this study was to determine how the drain fluid volume on the first day after surgery (DFV 1) can be used to predict clinically relevant post-operative pancreatic fistula following distal pancreatectomy (PD).Method: A retrospective analysis of 175 patients who underwent distal pancreatectomy in hepatobiliary surgery at Chengdu 363 Hospital (China) from January 2015 to January 2021 has been performed. Depending on the presence of pancreatic fistula, all patients were divided into two groups: POPF and non-POPF. In accordance with clinical impact on a patient's hospital course, the ISGPFD has defined three levels of POPF (grades A, B, C). The clinical factors were analyzed using SPSS 17.0 and Medcalc software. In order to assess the effectiveness of DFV 1 in predicting POPF after surgery, ROC curves were used to calculate its cut-off point. ﻿Result: Of the 175 patients who underwent distal pancreatectomy, 33 suffered with biochemical leaks, 28 (93.3%) with grade B POPFs, and 2 (6.7%) with grade C POPFs. The results from univariate and multivariate logistic regression analysis showed that the drain fluid volume on the first postoperative day (OR=0.99, P = 0.02) and the preoperative ALT level (OR=0.98, P =0.03) were independent risk factors associated with CR-POPF. Receiver operating characteristic (ROC) curve analysis showed that a drainage volume of 156 mL in 24 hours was an optimal threshold for the association with complications. The area under the curve was 0.624 (95% CI: 0.548-0.696). An average sensitivity and specificity of 46.67% and 76.55% were achieved respectively.﻿Conclusion: After distal pancreatectomy, the drainage volume on the first postoperative day can predict the presence of a clinically relevant pancreatic fistula.
Review on the Seasonal Malaria Chemoprevention implementation, effect on malaria incidence in a context of development of P. falciparum resistant genotypes with potential reduction of the effectiveness in western and central African countries
Authors: Nikiema Séni , Soulama Issiaka , Sombié Salif , Tchouatieu André-Marie , Serme Sindié Samuel , Henry Noélie , Ouaré Nathalie , Ily Raissa , Ouédraogo Oumarou , Zongo Dramane , Djigma Florencia Wendkuuni , Tiono Alfred B. , Sirima Sodiomon B. , Simpore Jacques 
Published: April 2022

Abstract
Seasonal Malaria Chemoprevention (SMC), which combines amodiaquine (AQ) with sulfadoxine-pyrimethamine (SP), is an effective and promising strategy, recommended by WHO, for controlling malaria morbidity and mortality in areas of intense seasonal transmission. Despite this strategy, the number of malaria cases in the target group, children under five years old, has increased while the implementation of SMC will be extended in several African countries. This increase in the prevalence of malaria cases suggests the need to evaluate SMC and understand some of the factors that may hinder the success of this strategy in the intervention areas. The present review discusses the impact of SMC on target population morbidity, parasite resistance to antimalarial drugs, molecular and the immunity affecting the incidence of malaria in children under five years old. This approach will contribute to improving the malaria control strategy in highly seasonal transmission areas.
Time to Remission of Juvenile Idiopathic Arthritis following Intraarticular Steroid Injection
Authors: Pratiwi Afrilia Intan , Julia Madarina , Satria Cahya Dewi , Sumadiono Sumadiono 
Published: April 2022

Abstract
Background: Juvenile Idiopathic Arthritis (JIA) is one of the most common chronic rheumatologic diseases of childhood and the ultimate treatment goal is disease remission with optimal physical functions. Intraarticular corticosteroid injection is widely used in children with JIA to relieve joint pain, swelling, and impaired mobility. Recently, there is a shift towards an early aggressive treatment with the intention to attenuate inflammation earlier since there may be a window of opportunity to prevent chronicity. This study aimed to investigate association between different onset of intraarticular corticosteroid injection and the duration of remission.Methods: A retrospective cohort study was conducted in Allergy and Immunology Clinic Dr. Sardjito Hospital Yogyakarta. Patients aged ≤ 16 years diagnosed JIA were included. Data were recorded from medical records. Survival analysis was used to identify time from first joint injection to the time of remission in patient with JIA.Results: Medical records from 115 JIA patients were reviewed from 2015 until 2020. 44% subjects had oligoarticular subtype and 43,47% achieved remission. Mean time to achieve remission in no injection, early injection (<3 months), and late injection (>3 months) groups were 24, 26, 39 months respectively (log rank p=0,090). Patient with NSAID monotherapy had higher probability to achieve remission as compared to other groups (HR 6.90, p=0,000). Conclusion: There was no difference in remission time between no injection, early injection, and late injection groups. There was also no significant difference in remission time between oligoarticular and non-oligoarticular subtype. Patient with JIA that need only NSAID monotherapy was the best predictor for remission.
Prediction of annual coffee production yield using artificial neural network and multiple linear regression techniques
Authors: Kittichotsatsawat Yotsaphat , Tippayawong Nakorn , Tippayawong Korrakot Yaibuathet 
Published: April 2022

Abstract
Crop yield and its prediction are crucial in agriculture production planning. This study investigates and analyzes annual coffee yield prediction in order to match the market demand, using artificial neural networks and multiple linear regression. Data were collected for six variables, including areas, productivity zones, rainfalls, relative humidity, and minimum and maximum temperature. The predicted yield of the cherry coffee crop continuously increases each year. It was found that the prediction accuracy of the R2 and RMSE were 0.9524 and 0.0642, respectively. The multiple linear regression showed potential in determining the relationship of cherry coffee yield.
Why Dentistry? A Qualitative Enquiry into Dental Students’ Perceptions in the State of Qatar.
Authors: Daud Alaa , Matoug-Elwerfelli Manal , Ali Kamran , Du Xiangyun 
Published: April 2022

Abstract
Background: Career choice is a complex, multifaceted process affecting all aspects of life. Motivational factors of aspiring dentists are crucial to inform institutions and aid with the admission process. The aim of this study was to investigate undergraduate dental students’ perceived motivation for their career choice in the first dental institution in the State of Qatar. Methods: Qualitative methods using face-to-face focus group sessions were utilized. All focus groups were conducted in English and contained a moderator and observer. Purposive sampling technique was employed to collect data from five focus groups of undergraduate dental students to gain a deeper insight into their motivation of career choice. A topic guide was used to ensure data collection standardization. Participants’ views were recorded and filed notes obtained. Data was transcribed and analysis performed utilizing an inductive thematic approach.Results: A total of 34 students (89.5%) from year 2 and 3 participated in the focus groups. Data analysis revealed six main themes emerging from this study namely; altruism and patient care, family influence, childhood aspirations, practical skills, professional and social status, and reconsideration and transfer opportunity. Altruism, opportunities to learn practical skills and professional status appeared to be the main drivers influencing their career choice. Conclusion: This qualitative study provided insightful information regarding current undergraduate dental students’ decision process involved in their profession selection. Students perceived their motivation to be patient care-centered, there was also a strong inclination towards performing practical hands-on tasks as a dentist, and developing a professional status. Interestingly, financial reward did not feature as a motivational factor in this study. This data could help the dental institutions better understand future applicant’s motivations to join dentistry and assist with the academic recruitment process and targeting students with a potential to excel in the profession.
Identification of hub genes and molecular genetic mechanisms in Alzheimer’s Disease and Schizophrenia
Authors: Zeng Yixuan , Zheng Yuxi , Ren Lijie , Chen Ting 
Published: April 2022

Abstract
Cognitive impairment and psychotic symptoms are both common in schizophrenia and Alzheimer's disease (AD), affecting more than half of the patients. Shared symptoms between AD and SCZ indicating similar cerebral pathophysiologies exist, common in both clinical and subclinical settings. Neurochemical alterations of the dopaminergic/cholinergic axis are important in schizophrenia and AD, as well as high-risk genes. Insights drawn from these observations enrich the understanding of the relationship between schizophrenia and AD, helping to further the symptomatic control of each disease.