bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spiro-3-indolin-2-ones: Synthesis, biological properties and computational studies
Authors: Fawazy Nehmedo G. , Panda Siva S. , Mostafa Ahmed , Kariuki Benson M. , Bekheit Mohamed S. , Moatasim Yassmin , Kutkat Omnia , Fayad Walid , El-Manawaty May A. , Soliman Ahmed A. F. , El-Shiekh Riham A. , S Aladdin M. , Barghash Reham F. , Girgis Adel S. 
Published: June 2022

Abstract
A series of 1''-(alkylsulfonyl)-dispiro[indoline-3,2'-pyrrolidine-3',3''-piperidine]-2,4''-diones 6a‒o has been synthesized through regioselective multi-component azomethine dipolar cycloaddition reaction of 1-(alkylsulfonyl)-3,5-bis(ylidene)-piperidin-4-ones 3a‒h. X-ray diffraction studies (6b‒d,h) confirmed the structures. The majority of the synthesized analogs reveal promising antiproliferation properties against a variety of human cancer cell lines (MCF7, HCT116, A431 and PaCa2) with good selectivity index towards normal cell (RPE1). Some of the synthesized agents exhibit potent inhibitory properties against the tested cell lines with higher efficacies than the standard references (sunitinib and 5-fluorouracil). Compound 6m is the most potent. Multi-targeted inhibitory properties against EGFR and VEGFR-2 have been observed for the synthesized agents. Flow cytometry supports the antiproliferation properties and shows the tested agents as apoptosis and necrosis forming. Vero cell viral infection model demonstrates the anti-SARS-CoV-2 properties of the synthesized agents. Compound 6f is the most promising (about 3.3 and 4.8 times the potency of the standard references, chloroquine and hydroxychloroquine). QSAR models explain and support the observed biological properties.
Hanshiyi Formula treatment in patients with COVID-19: study protocol and statistical analysis plan (SAP) of an open-label randomized controlled clinical trial
Authors: Jin Meiying , Ren Jixiang , Ma Jing , Gao Zezheng , Wei Yu , Han Lin , Liu Lei , Zhang Xiaowen , Zheng Xu , Su Jing , Liu Yangyang , Tang Cheng , Ding Qiyou , Yao Chensi , Bao Tingting , Yang Haoyu , Lin Jianran , Chen Keyu , Chen Jia , Gou Xiaowen , Zhou Danni , Kang Mengjiao , Zhao Linhua , Zhu Guoqi , Deng Yongzhi , Zhang Ying 
Published: June 2022

Abstract
Background
As COVID-19 continues to spread rapidly in 2019, the risks to human health cannot be underestimated. There is still lack of antiviral drugs for COVID-19, and the development of effective treatments is urgent. Traditional Chinese Medicine (TCM) has been widely used in the treatment of epidemic infectious diseases, The purpose of this randomized controlled clinical trial will to evaluate the clinical efficacy and safety of Hanshiyi Formula (HSYF) in patients with COVID-19.
Methods
We will conduct this randomized, controlled, open, non-inferiority test clinical study in 240 COVID-19 subjects. Eligible patients will be divided into a control group ( LH granule ) or a treatment group ( HSYF ) in a 1:1 ratio. The intervention duration will be 14 days, and the medication could be stopped in advance if the discharge standard was reached. Clinical efficacy measures will be analyzed at baseline and on day 1–14 of enrollment, The occurrence of adverse events will be monitored throughout the trial. The statistical analysis plan included the treatment of missing data, the analysis of outcome measures, and the method of safety endpoints.
Discussion
To investigate the efficacy and safety of HSYF in the treatment of COVID-19, provide more sufficient high-quality evidence-based medical basis for TCM intervention in the diagnosis and treatment of COVID-19. To avoid selective reporting bias and data-driven analysis, the statistical analysis plan will standardize the statistical analysis of clinical trials.
Trial registration:
Chinese Clinical Trial Registry, ChiCTR2200058384. Version V1.2, created on April 11, 2022.https://www.chictr.org.cn/com/25/hvshowproject.aspx?id=159938.
Influence of the Surface Modification of Granular Activated Carbon Sintetized from Macauba on Heavy Metals Sorption
Authors: Rios Regiane D. F. , Bueno Patrícia J. B. , Terra Júlio C. S. , Moura Flávia 
Published: June 2022

Abstract
Adsorption on activated carbon is a promising technique for treatment of low-concentration heavy metal pollutants in water with high efficiency and simple operation. However, commercial activated carbon is often associated with high costs. Therefore, much attention has been given to activated carbon derived from low-cost agricultural and residual biomass. In this work, adsorption of Zn, Cd, and Pb ions in aqueous solutions were conducted using granular activated carbon obtained from macauba palm, biomass waste of biofuel production, after surface modification using different methods. The adsorbents were obtained in granular form which facilitates all steps of the use, recovery and reuse of the material, differently from the powdered activated carbon normally used. The materials were characterized by using XPS, elemental analysis, N2 sorption (BET method) and zeta potential measurements and presented high adsorption capacities of the metals when compared to other works in the literature.
Myanmar’s Economic Structure during the Transition Period
Authors: THEIN EI EI , Inaba Kazuo 
Published: June 2022

Abstract
Following the political and economic reform in 2011, Myanmar’s economy has been internationally recognized as having developing status with over 7% average GDP growth. This study’s main objective is to examine Myanmar’s basic economic structure and sectoral expansion during the economic reformation between 2010 and 2015 in comparison to those of other ASEAN countries. The data for the analyses are taken from the Eora global database and the Asian Development Bank’s (ADB’s) database. During 2010–2015, the gross production expansion of Myanmar was 1.78 times, the highest growth record among the selected countries. Despite its high economic growth, Myanmar’s economic structure does not show remarkable change within this period. Domestic demand dominates Myanmar’s output growth and import and export shares in gross production are very low (around 1% export and 4% import). The petroleum, chemical and nonmetallic mineral industry shows high forward linkages. On the other hand, backward linkages are high in transport equipment. Analysis of sectoral deviations from proportional growth (DPGs) shows that Myanmar’s production growth is mainly attributed to the public administration and construction sectors, which boom because of increased investment and consumption. Keywords: Economic structure, sectoral expansion, forward linkages, backward linkages, deviations from proportional growth
Determining the effective dose of esketamine for mitigating pain during propofol injection by the sequential method
Authors: zeng wei , Tan Meiyun , Zhang Chunyuan , Chen Maofang , Huang Zehui , Huang Ding 
Published: June 2022

Abstract
BackgroundPropofol is an intravenous (IV) anesthetic medication widely used for procedural sedation, operative anesthesia, and in intensive care units (ICUs), but the incidence of pain during IV infusion can reach 28–90%. Ketamine can attenuate pain associated with IV propofol injection through local and central analgesic effects. Ketamine is gradually being transitioned to its S-enantiomer, esketamine, which has a similar mechanism of action. The purpose of our study is to determine the half effective dose (ED50), 95% effective dose (ED95), and 99% effective dose (ED99) of esketamine for attenuating propofol injection pain using the sequential method to provide a reference for optimal dose selection for surgeries and procedures.MethodsWe selected 30 patients, 18–40 years old, who were scheduled to undergo hysteroscopy in the gynecology department of our hospital from June 2021 to June 2022, with a body mass index (BMI) of 18.5–24.9 kg/m2; Mallampati class I or II; and American Society of Anesthesiologists (ASA) Class I or II. Esketamine combined with propofol was used for general anesthesia induction. During induction, the target dose of esketamine was first given via venous access in the left hand of the patient, and 30 s later, a fixed dose of 2 mg/kg (1 ml/s) of propofol was given. Patient perception of pain was scored with the verbal rating scale (VRS) every 5 s after the start of the propofol infusion, and the evaluation was stopped once the patient became unresponsive. The esketamine dose was increased or decreased based on patient’s pain response using the sequential method. The initial dose was 0.2 mg/kg and the subsequent sequential dose was 0.02 mg/kg.ResultsThe ineffective group comprised patients with a positive pain response and the effective group comprised patients with a negative pain response. We found esketamine’s ED50 = 0.143 mg/kg (0.120, 0.162 mg/kg), ED95 = 0.176 mg/kg (0.159, 0.320 mg/kg), and ED99 = 0.189 mg/kg (0.167, 0.394 mg/kg). The esketamine dose and VRS score during propofol injection were significantly different between the two groups (P < 0.05), whereas surgical duration, emergence time, visual analogue scale (VAS) score of postoperative uterine contraction pain, and Riker sedation/anxiety scale (SAS) score were not significantly different. All patients were hemodynamically stable at induction and we observed no obvious adverse reactions.ConclusionSmall doses of esketamine combined with propofol can be safely and effectively used for hysteroscopic surgery. We recommended a dose of 0.2 mg/kg IV esketamine before induction of anesthesia to reduce the pain of propofol injection.Trial registrationChinese Clinical Trial Registry ChiCTR2100048951. Date of registration: July 19, 2021.
The Implication of Spatial-Dispersion Theory in Housing Genotypes of Boushehri Historic and Contemporary Houses
Authors: Nadoomi Rahil , Sharghi Ali , Sheybani Mehdi , Pour Bahram Saleh Sedgh 
Published: June 2022

Abstract
Home has always been an important topic in research. In each region, houses change over time under different economic, social, cultural, climatic, and other conditions. Although these gradual changes have occurred to improve the spatial conditions of houses, it has led to a decline in the environmental quality and sometimes their technical efficiency. It is possible to redefine contemporary housing by spatially analyzing houses and discovering genotypes in a particular climate. This study uses space syntax algorithms in the syntactic plugin in Grasshopper to conduct various analyses and combine them with visual analysis extracted from Depth Map. House genotypes are discovered by factor analysis in SPSS. The case study is the coastal and historical city of Bushehr in the south of Iran. The sample size is 45 houses from 3historical periods selected by non-random purposeful sampling. The results indicate that the houses in Bushehr have 4genotypes, each of which has positive characteristics that can be generalized to contemporary housing architecture.
NADH oxidase of Mycoplasma synoviae is a potential diagnostic antigen, plasminogen/fibronectin binding protein and a putative adhesin
Authors: Hu Zengjin , Li Haoran , Wang Guijun , Shang Yuanbing , Chen Yuetong , Zhao Yuxin , Wang Shaohui , Tian Mingxing , Qi Jingjing , Yu Shengqing 
Published: June 2022

Abstract
Background
Mycoplasma synoviae (MS) is an important pathogen that causes respiratory diseases and arthritis in chickens and turkeys, resulting in serious economic losses to the poultry industry. Membrane-associated proteins were thought to play important roles in cytoadherence and pathogenesis. NADH oxidase (NOX) is a kind of oxidoreductase involved in glycolysis, which was thought to be a multifunctional protein and a potential virulence factor in some pathogens. However, there was still little knowledge about the NOX of MS (MSNOX). Our previous study has proved that the MSNOX was not only a cytoplasmic metabolic enzyme, but was also distributed in MS membrane. This study was mainly to explore the potential as a diagnostic antigen and an adhesion of the NOX in MS.
Results
In this study, Western blot analysis showed that recombinant MSNOX (rMSNOX) protein could react with positive sera against different MS isolates, but not MG isolates or other avian pathogens, suggesting the rMSNOX is a potential diagnostic antigen. In addition, the rabbit anti-rMSNOX serum showed a complement-dependent mycoplasmacidal rate as high as 86.5%. MS NOX protein has been identified as a cytoplasmic enzyme in our previous study, it was identified to be distributed on the surface of MS in this study using suspension immunofluorescence assays. Indirect immunofluorescence assays and colony counting assays showed that the rMSNOX and MS could adhere to DF-1 cells and the adherence could be significantly inhibited by the rabbit anti-rMSNOX serum. Moreover, plasminogen (Plg)- and fibronectin (Fn)-binding assays showed that rMSNOX was able to bind to Plg and Fn in a dose-dependent manner, further confirmed that MSNOX could be a putative adhesin.
Conclusion
The MSNOX was identified to be a surface immunogenic protein which also has good immunoreactivity and specificity, suggesting it may be used as a potential diagnostic antigen in future. The rMSNOX and MS presented adherence to DF-1 cells, which were inhibited by the rabbit anti-rMSNOX serum. Moreover, rMSNOX showed cytoadherence to extracellular matrix (ECM) proteins including Plg and Fn, suggesting that the NOX may play important roles in cytoadherence and pathogenesis of MS.
Van der Waals interactions regulating the hydration of 2-methacryloyloxyethyl phosphorylcholine, the constructing monomer of biocompatible polymers
Authors: Takahashi Masae , Chen Sifan , Matsui Hiroshi , Morimoto Nobuyuki , Ikemoto Yuka 
Published: June 2022

Abstract
Van der Waals (VDW) interactions provide fantastic properties for biological systems that function at room temperature. The VDW interaction, which primarily contributes to weak hydrogen bonding, is expected to play a key role in regulating hydrophobic hydration to express the biologically inert biocompatible function of polymerized MPCs (2-methacryloyloxyethyl phosphorylcholine). This report explores the biologically inert function of polymerized MPCs at the molecular level through an array of vibrational spectroscopic and computational characterization of MPC monomers as temperature-dependent change of intramolecular weak hydrogen bonding. Synchrotron Fourier transform infrared microspectroscopy and terahertz time-domain spectroscopy were used to investigate temperature-dependent spectral changes in the low-frequency vibrations of the MPC over the temperature range from cryogenic to room temperature, and the results were analyzed by highly reliable and well-established density functional theory (DFT) calculations. Complicated spectral features in the low-frequency energy region and the uncertain conformations of the MPC in the amorphous powder state are clearly resolved under a polarizable continuum model and dispersion correction to pure DFT calculations.
Reducing VEGFB accelerates NAFLD and insulin resistance in mice via inhibiting AMPK signaling pathway
Authors: Li Rongrong , Li Yuqi , Yang Xueling , Hu Yaorui , Yu Haining , Li yana 
Published: June 2022

Abstract
Objective: Vascular endothelial growth factor B (VEGFB) was regarded to improve lipid metabolism and reduce obesity-related hyperlipidemia. Whether VEGFB participates in lipid metabolism in nonalcoholic fatty liver disease (NAFLD) has not been clear yet. This study investigated the involvement of VEGFB in lipid metabolism and insulin resistance via the AMPK signaling pathway in NAFLD. Methods: We constructed the animal and cell model of NAFLD after VEGFB gene knockout to detect liver damage and metabolism in NAFLD. Bioinformatics analysis of VEGFB and the AMPK signaling pathway relative genes to verify the differential proteins. And mRNA levels of NAFLD fatty acid metabolism-related genes were detected.Results: Our study found that after high-fat diet (HFD), body fat content, serum lipoprotein and NAFLD scores of VEGFB knockout mice increased, and insulin resistance was distinct. The phosphorylation level of proteins in the AMPK / ACC / CPT1 was descended after reducing VEGFB, so as to inhibit the oxidation of fatty acids in hepatocytes mitochondria.Conclusion: Consequently, reducing VEGFB also increased the expression level of proteins in the AMPK / SREBP1 / Scd1 pathway and resulted in endoplasmic reticulum lipid synthesis.
The Correlation Study of C5 Nerve Palsy after Anterior Cervical Decompression and Fusion：A Retrospective Study
Authors: Wang Liang , Jiang Jiayao , Zhao Hongbo , Zhang Bangke , Lu Xuhua , Liu Tielong 
Published: June 2022

Abstract
Background: Anterior cervical decompression and fusion (ACDF) is the gold standard surgical treatment for cervical degenerative disease. Postoperative C5 nerve root palsy is a known complication following cervical spine surgery. The etiology of postoperative C5 nerve root palsy remains unknown. This study was performed to determine the characteristics that may predict a risk of developing C5 palsy in patients following anterior cervical decompression and fusion (ACDF) as measured by cervical vertebral lateral radiography and magnetic resonance imaging (MRI).Methods: Radiographic parameters were measured by cervical vertebral lateral radiography and magnetic resonance imaging (MRI) and recorded. The anteroposterior diameter (APD), left and right ipsilateral foraminal diameters, and left and right cord-lamina angles were measured on preoperative MRI of the cervical vertebra and recorded. Additionally, we measured two parameters on preoperative and postoperative (within 2 weeks of the procedure) lateral plain-film radiographs: the cervical spine angle and cervical lordosis.Results: 21 patients with C5 nerve palsy and 175 controls were identified. There were no baseline differences in the left and right foraminal diameters. The APD, left and right cord-lamina angles, and cervical curvature correction were significantly different between the two groups (P < 0.05).Conclusions: For patients with ACDF, a smaller APD and excessive cervical curvature are correlated with C5 palsy. Excessive reconstruction of cervical curvature should not be performed in patients with preoperative foraminal narrowing.
Biallelic loss of LDB3 leads to a lethal pediatric dilated cardiomyopathy
Authors: Jamshidi Yalda , Koopmann Tamara , Naghibi-Sistani Mohammadreza , Klift Heleen M. van der , Birjandi Hassan , Al-Hassnan Zuhair , Alwadai Abdullah , Zifarelli Giovanni , Karimiani Ehsan Ghayoor , Sedighzadeh Sahar , Bahreini Amir , Nouri Nayereh , Peter Merlene , Watanabe Kae , Duyvenvoorde Hermine van , Ruivenkamp Claudia , Teunissen Aalbertine , Harkel Arend Ten , Duinen Sjoerd van , Haak Monique , Prada Carlos , Santen Gijs , Maroofian Reza 
Published: June 2022

Abstract
Autosomal dominant variants in LDB3 (also known as ZASP), encoding the PDZ-LIM domain-binding factor, have been linked to a late onset phenotype of cardiomyopathy and myofibrillar myopathy in humans. However, despite knockout mice displaying a much more severe phenotype with premature death, bi-allelic variants in LDB3 have not yet been reported. Here we identify biallelic loss-of-function variants in five unrelated cardiomyopathy families by next generation sequencing. In the first family we identified compound heterozygous LOF variants in LDB3 in a fetus with bilateral talipes and mild left cardiac ventricular enlargement. Ultra-structural examination revealed highly irregular Z-disc formation, and RNA analysis demonstrated little/no expression of LDB3 protein with a functional C-terminal LIM domain in muscle tissue from the affected fetus. In a second family a homozygous LDB3 nonsense variant was identified in a young girl with severe early onset dilated cardiomyopathy with left ventricular non compaction; the same homozygous nonsense variant was identified in a third unrelated female infant with dilated cardiomyopathy. We further identified homozygous LDB3 frameshift variants in two unrelated probands diagnosed with cardiomegaly and severely reduced left ventricular ejection fraction. Our findings demonstrate that recessive LDB3 variants can lead to an early-onset severe human phenotype of cardiomyopathy and myopathy, reminiscent of the knockout mouse phenotype, and supporting a loss of function mechanism.
Predicting Mechanical Properties of Material Extrusion Additive Manufacturing-Fabricated Structures with Limited Information: Harnessing Entanglement Theory and Modeling
Authors: Peterson Amy M. , Kazmer David O. 
Published: June 2022

Abstract
Mechanical properties of additively manufactured structures fabricated using material extrusion additive manufacturing are predicted through combining thermal modeling with entanglement theory and molecular dynamics approaches. A one-dimensional model of heat transfer in a single road width wall is created and validated against both thermography and mechanical testing results. Various model modifications are investigated to determine which heat transfer considerations are important to predicting properties. This approach was able to predict tear energies on reasonable scales with minimal information about the polymer. Such an approach is likely to be applicable to a wide range of amorphous and low crystallinity thermoplastics.
Panel containing four serum microRNAs has potential to diagnose bladder cancer
Authors: Li Xinji , Lu Chong , Wen Zhenyu , Li Rongkang , Chen Xuan , Huang Guocheng , Li Hang , Tao Lingzhi , Hu Yimin , Zhao Zhengping , Chen Zebo , Wu Xionghui , Lai Yongqing 
Published: June 2022

Abstract
Background
Bladder cancer (BC) is one of the ten most common cancers in the world with late detection and low age of onset. There is abundant evidence that for the management of BC, the earlier detection and timely intervention, the better the prognosis. Substantial evidence indicates that microRNAs (miRNAs) are specific to different tumor types and have remarkable stability, so serum miRNAs can serve as potential cancer diagnostic markers.
Methods
The serum samples including 112 BC patients and 112 non-cancer controls (NCs) were gathered as research objects and detected with quantitative reverse transcription-PCR (qRT-PCR) for verifying the discrepant miRNAs. A panel containing multi-miRNAs was established with superior diagnostic performance. Database of TCGA and KEGG was exerted for survival analysis and bioinformatics analysis, respectively.
Results
Six serum miRNAs (miR-221-5p, miR-181a-5p, miR-98-5p, miR-15a-5p, miR-222-3p and miR-197-3p) were significantly aberrant in BC patients, among which four miRNAs (miR-221-5p, miR-181a-5p, miR-15a-5p, miR-222-3p) were assembled into a panel with high diagnostic value (AUC = 0.875) based on logistic regression analysis. Survival analysis showed that miR-181a-5p was closely related to BC prognosis.
Conclusion
The four-miRNAs combination (miR-221-5p, miR-181a-5p, miR-15a-5p and miR-222-3p) may become a novel non-invasive serological biomarker for BC screening.