bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of the efficacy of drug-eluting balloons in coronary stenotic lesions combined with distal myocardial bridges
Authors: wu zhihao , Wang Si , Peng Yang-wei , Xie Jun-min , Jia Xin-wei 
Published: April 2022

Abstract
objectives To assess the efficacy and safety of drug-coated balloons (DCB) in treating coronary artery stenosis combined with a distal myocardial bridge (MB).Methods A total of 67 patients were enrolled with anterior descending coronary artery stenosis >70% combined with distal MB and were randomized into the DCB group (n=33) and the Drug-Eluting Stent (DES) group (n=34). All patients completed PCI under intravascular ultrasound guidance, the operation time, contrast agent dosage, and x-ray radiation dosage was compared between the two groups, observing intraoperative lumen area variations, intimal dissection, and vascular elastic recoil. Coronary angiography was rechecked at 12 months. The major adverse cardiac events (MACEs), hemorrhagic events, and the quantitative coronary angiography analysis results were compared between the two groups.Results The DCB group had less operative time, contrast dosage, and X-ray radiation than the DES group (P<0.05); There was one case of C-type dissection and one case of immediate postoperative vascular elastic recoil, both of which were remedial stent implantation. DES group superior to DCB group for immediate postoperative lumen increase (p<0.001). LLL was significantly smaller in the DCB group than in the DES group (-0.04±0.04 mm vs. 0.18±0.06 mm, p<0.001), and the restenosis was higher in the DES group than in the DCB group (6.7% vs. 26.7%, p=0.038). The MACEs in the DCB group were lower than that in the DES group (9.7% vs. 33.3%, p=0.022), especially rates of TLR (6.5% vs.27.3%,p=0.027); There were no major hemorrhagic events in either group, and mild bleeding event rate between the two groups was not statistically significant(p=0.298).Conclusion Coronary stenosis combined with distal MB is treated safely and effectively with DCB and has better long-term results than DES.
Changes of the Gut Microbiota Composition in Patients with Atrial Fibrillation
Authors: Qu Xiang , Chen Jinxin , Huang Yuheng , Wu Yanran , Li Junfeng , Ni Weicheng , Lu Yucheng , Li Zhenzhen , Chen Lingzhi , Zhao Chuhuan , Kong Shuting , Zhou Hao 
Published: April 2022

Abstract
Background With the establishment of the cardiac-gut axis concept, increasing evidence has suggested the involvement and important regulatory role of the gut microbiota (GM) in cardiovascular diseases. Whereas, the relationship between GM and atrial fibrillation (AF) still poorly understood.Objectives The aim of this study was to investigate whether there were differences in GM between AF patients and healthy controls, and to discover the factors that affecting the changes of GM.Methods In this study, we enrolled 30 hospitalized patients with AF and 30 matched patients with sinus rhythm (SR). GM species in fecal samples were evaluated through amplicon sequencing targeting the 16Sribosomal RNA gene. GM species richness, diversity, differential abundance of individual taxa between AF and SR were analyzed.Results AF patients showed decreased species richness and α-diversity compared to SR patients, but there was no statistical difference. The phylogenetic diversity was significant decreased in AF group (P<0.001). The β-diversity indexes revealed significant differences in GM community structure between AF group and SR group. After investigated the individual taxa, AF group showed altered relative abundance in several taxa compared to the SR group. linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed, a significant decrease in Bifidobacterium and a greater abundance of Lactobacillus, Fusobacterium, Haemophilus in AF group compared with the SR group.Conclusions In AF patients, the GM phylogenetic diversity and β-diversity decreased, the relative abundance altered in several taxa and the bacterial community structure changed. GM dysbiosis might play a crucial part in the occurrence and development of AF.Clinical Trial Registration: chictr.org.cn identifier: ChiCTR2100050063
Non-age-related Gait Kinematics and Kinetics in the Elderly
Authors: Liang Yuanhao , Xu Tinghan , Qi Shichen , Cao Xiang , Yeung Eric Hiu Kwong , Hu Yong 
Published: April 2022

Abstract
Background: Non-age-related gait kinematics and kinetics are essential indicators for gait with normal function. They are instrumental in clinical evaluation and assistant device design. However, only a few studies focus on non-age-related gait analysis. This study aims to identify the non-age-related gait kinematics and kinetics by comparing their normalized time-varying waveforms in the healthy elderly and young groups. Methods: Gait analysis for each gait cycle at self-paced is conducted from marker trajectories and ground reaction force. Pattern distance and percentage of significant difference between the young and elderly are calculated to represent the two sets of waveforms. The k-means clustering and elbow method are used to select and validate non-age-related kinematics and kinetics. The average waveforms with standard deviation are plotted for the comparison of the results. Results: There is no significant difference in weight and height between two aging groups. The elbow point is where the k value equals two. The cluster centers of the two groups are 0.1417 and 0.3691 while total Euclidean distances are 0.001794 and 0.02750, respectively. The two critical values closest to the cutoff are 0.1593 and 0.3037. The average waveforms of the non-age-related group are highly overlapped with a minor standard deviation between healthy young and elderly groups but show lager variations between healthy and abnormal groups. Conclusions: Ankle moment, knee angle, hip flexion angle, and hip adduction moment are identified as the non-age-related gait kinematic or kinetic features with distinguishing cutoff. These features are validated to reflect abnormal walking function, which is essential in the evaluation of mobility and functional ability of the elderly, and data fusion of the assistant device.
The Anti-estrogen Receptor Drug, Tamoxifen, is Selectively Lethal to P- glycoprotein-expressing Multidrug Resistant Tumor Cells
Authors: Bakadlag Rowa , Limniatis Georgia , Georges Elias 
Published: April 2022

Abstract
Background P-glycoprotein (P-gp), a member of the ATP Binding Cassette B1 subfamily (ABCB1), confers resistance to clinically relevant anticancer drugs and targeted chemotherapeutics. However, paradoxically P-glycoprotein overexpressing drug resistant cells are “collaterally sensitive” to non-toxic drugs that stimulate its ATPase activity. MethodsCell viability assays were used to determine the effect of low concentrations of tamoxifen on the proliferation of multidrug resistant cells (CHORC5 and MDA-Doxo400), expressing P-gp, their parental cell lines (AuxB1 and MDA-MB-231) or P-gp-CRISPR knockout clones of AuxB1 and CHORC5 cells. Western blot analysis was used to estimate P-gp expression in different cell lines. Apoptosis of tamoxifen-induced cell death was estimated by flow cytometry using Annexin-V-FITC stained cells. Oxidative stress tamoxifen treated cells was determined by measuring levels of reactive oxygen specifies and reduced thiols using cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) and 5,5-dithio-bis-(2-nitrobenzoic acid) DTNB, respectively. ResultsIn this report, we show that P-gp-expressing drug resistant cells (CHORC5 and MDA-Doxo400) are collaterally sensitive to the anti-estrogen tamoxifen or its metabolite (4-hydroxy-tamoxifen). Moreover, P-gp-knockout clones of CHORC5 cells display complete reversal of collateral sensitivity to tamoxifen. Drug resistant cells exposed to low concentrations of tamoxifen show significant rise in reactive oxygen species, drop of reduced cellular thiols and increased apoptosis. Consistent with the latter, CHORC5 cells expressing high levels of human Bcl-2 (CHORC5-Bcl-2) show significant resistance to tamoxifen. In addition, the presence of the antioxidant N-acetylcysteine or P-gp ATPase inhibitor, PSC-833, reverse the collateral sensitivity of resistant cells to tamoxifen. By contrast, the presence of rotenone (specific inhibitor of mitochondria complex I) synergizes with tamoxifen. ConclusionThis study demonstrates the use of tamoxifen as collateral sensitivity drug that can preferentially target multidrug resistant cells expressing P-gp at clinically achievable concentrations. Given the widespread use of tamoxifen in the treatment of estrogen receptor-positive breast cancers, this property of tamoxifen may have clinical applications in treatment of P-gp-positive drug resistant breast tumors.
Effect of chlorhexidine acetate on the early postoperative orthotopic ileal neobladder in situ mucus secretion
Authors: kang Peng , Raj Ashok , Renfu Chen , Yunpeng Peng , Wenjing Xie , wang Fu jian , Xiaolei Sun 
Published: April 2022

Abstract
Objective: To investigate the impact of chlorhexidine acetate's on the mucus secretion function in the early postoperative period of the neo-ileal bladder and compare other treatment agents. Method: 24 patients who underwent total Cystectomy with orthotopic ileal neobladder in our department and divided into three groups (8 each). During the procedure, mucosal treatment agents were infused for five minutes, and specimens were taken for histological examination of the neobladder mucosa under light and electron microscopy.Results: The daily intestinal mucus secretion in the early stage of the neobladder(POD 3-14). Group A (Iodophor) compared to B (anhydrous ethanol), and C (chlorhexidine acetate) were reduced and statistically significant (P <0.05), with no statistical difference between B and C (P> 0.05). Light and Electron microscopy; In group A, the neobladder intestinal mucosa layer is complete; villi were uniformly arranged. A few goblet cells between the apex of the villi disappear an acceptable arrangement of micro-villi and a relatively low degree of organelle damage. In group B, the neobladder intestinal mucosa layer is unclear, the villi arrangement lost and shedding, muscle layer structure disrupted, accompanied by RBC and inflammatory cells infiltration. In group C, neo-bladder intestinal mucosa structure was complete, and villi are low, rupture, shedding, and inflammatory cells infiltrated without significant damage muscle layer.Conclusion: As a new mucous membrane treatment agent, chlorhexidine acetate has a protective effect on the mucous membrane of the new bladder. Meanwhile, it also inhibits the secretion of mucous in the new bladder in the early postoperative period.
Pan-Cancer Analysis Identifies YTHDF2 as an Immunological and Prognostic Biomarker
Authors: weiwei liu , Liu Chaoqun , Chen Zilin , Qian Cheng , lin wandie , Zhao Liang , Zhou Rui 
Published: April 2022

Abstract
Background
N6-methyladenosine (m6A) modification is a dynamic and reversible post-transcriptional RNA modification prevalent in eukaryotic cells. As a member of m6A reader protein, YT521-B homology domain family 2 (YTHDF2) has been demonstrated involving in many vital biological process, whereas its role in cancers remains unclear.
Methods
We evaluated the immunohistochemical staining of tissue sections from 51 hepatocellular carcinoma patients and TIMER database to investigate the association between the YTHDF2 expression and the infiltration of immunocytes. TCGA, GEPIA2, GEO and GTEx databases were used to analyze the differential expression of YTHDF2 in various cancers. R 3.6.3 is used for Data visualization.
Results
YTHDF2 is overexpressed in most tumors, and the increased YTHDF2 expression is associated with poor OS, PFI, and DSS. YTHDF2 expression is also positively correlated with MMR, TMB and MSI. Moreover, GSEA revealed that functions associated with cell adhesion, cell cycle and immune regulation were enriched in YTHDF2 overexpression tumors. Further study verified the participation of YTHDF2 in immune regulation through its correlation with ICP genes and the infiltration of immunocytes in tumor microenvironment. Notably, we demonstrated a positive correlation between YTHDF2 expression and the infiltration of CD8+ T cells and macrophages, as well as exhausted T lymphocytes.
Conclusion
YTHDF2 can be used as a new therapeutic target and a potential biomarker for cancer immune evasion and poor prognosis.
Research on ecological flow and water replenishment threshold for diversion river based on MC-LOR
Authors: Hu Wentong , Gu Wenquan , Miao Donghao , Shao Dongguo 
Published: April 2022

Abstract
Diversion rivers are important water and biological connection channels, which provide valuable ecological services. However, diversion rivers are degraded owing to the construction and unreasonable operation of large-scale water conservancy projects. Quantifying and guaranteeing the ecological flow of diversion rivers is necessary to sustain the riverine ecosystems. Due to the lack of methods to verify the rationality of the existing hydrological series length for ecological flow calculation by hydrological methods, a Monte Carlo-length of record (MC-LOR) algorithm was proposed to analyze the accuracy level of the existing hydrological series. Taking the Dongjing River (DJR) in the lower Han River as an example, the result shows that the 15-year hydrological series can meet the accuracy level of 5/90 and 10/95. The flow changes and hydrological relationships between the Qianjiang hydrological station (QJS) and the Tianguan River (TGR) under the influence of the Middle Route of South-to-North Water Transfer (MSNW) project and the Yangtze–Han Water Diversion (YHWD) project were analyzed. It is found that the flow variation of QJS is large and shows a downward trend while TGR displays a significant upward trend through the M-K trend test. And the flow of QJS is drier and less than the water replenishment of TGR in the non-flood season after the operation of the MSNW project. This paper established an ecological flow threshold calculation method based on kernel density curves and quantiles. Compared with the minimum average monthly flow (MAMF) method, the monthly minimum ecological runoff (MMR) method, improved Q90 method, Tennant method, Tessmann method, distribution flow method (DFM), the method proposed in this paper can reduce the influence of extreme flow events and is suitable for the diversion river. Finally, the ecological water replenishment demands of TGR to meet the ecological flow target of DJR under different flow conditions in the Han River were analyzed by Bayesian network (BN) models. The results show that the minimum water requirement thresholds of DJR are 31.3m3/s and 15.85m3/s, 55.35m3/s and 30.85m3/s when the flows of Han River are < 654m3/s and 654 ~ 770m3/s in non-flood season and < 875m3/s and 875 ~ 1210m3/s in flood season, respectively. This research provides references for the ecological stability and sustainable development of diversion rivers.
Poor body condition is associated with lower hippocampal neurogenesis and higher gut methanogen abundance in adult laying hens from two housing systems
Authors: Armstrong Elena A. , Richards-Rios Peter , Addison Lucy , Sandilands Vicky , Guy Jonathan H. , Wigley Paul , Boswell Tim , Smulders Tom V. 
Published: April 2022

Abstract
It is still unclear which commercial housing system provides the best quality of life for laying hens. In addition, there are large individual differences in stress levels within a system. Hippocampal neurogenesis may provide an integrated biomarker of the stressors experienced by an individual. We selected 12 adult hens each with good and poor body condition (based on body size, degree of feather cover and redness of the comb) from a multi-tier free range system (H&N hens) and an enriched cage system (Hy-Line hens) (n=48). Immature neurons expressing doublecortin (DCX) were quantified in the hippocampus, contents of the caecal microbiome were sequenced, and expression of inflammatory cytokines was measured in the spleen. DCX+ cell densities did not differ between the housing systems. In both systems, poor condition hens had lower DCX+ cell densities, exhibited elevated splenic expression of interleukin-6 (IL6) mRNA, and had a higher relative caecal abundance of methanogenic archea Methanomethylophilaceae. The findings suggest that a greater degree of cumulative chronic stress is experienced by those individuals in the poorest condition, and that a survey of the proportion of hens experiencing poor body condition might be one way to evaluate the impact of housing systems on hen welfare.
Adaptive Fault-Tolerant Visual Control of Robot Manipulators Using An Uncalibrated Camera
Authors: Yang Liang , Yuan Chan , Lai Guanyu 
Published: April 2022

Abstract
In this paper, we propose an adaptive faulttolerant visual control scheme for robotic manipulators with possible actuator failures in an uncalibrated environment. Most existing visual control approaches for robot system does not take into acount actuator failures, which may prominently a ect the transient performance of the system in practice. In order to moderate the detrimental and adverse e ects of actuator failures on the system, a new adaptive algorithm is proposed to compensate stuck-type failures occurred in actuators. Moreover, by proposing a decoupling method, the uncertain parameter model of actuator failure is successfully separated from the dynamics model while the two models are coupled in most existing results(e.g. [33], [34]). And the stability of the dynamic system and the convergence of the image error are proved by the Lyapunov analysis method. Finally, the proposed control scheme is applied to simulation model of 3-DOF manipulator, and the e ectiveness and stability of the controller is veri ed by comparing and analysing the tracking performance of the manipulator under di erent failure parameters.
Fine mapping of a stripe rust resistance gene YrZM175 in bread wheat
Authors: Wu Jingchun , Xu Dengan , Fu Luping , Wu Ling , Hao Weihao , Li Jihu , Dong Yan , Wang Fengju , Wu Yuying , He Zhonghu , Si Hongqi , Ma Chuanxi , Xia Xianchun 
Published: April 2022

Abstract
Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is a worldwide wheat disease that causes large losses in production. Fine mapping and cloning of resistance genes are important for accurate marker-assisted breeding. Here, we report the fine mapping and candidate gene analysis of stripe rust resistance gene YrZM175 in a Chinese wheat cultivar Zhongmai 175. Fifteen F1, 7,325 F2 plants and 117 F2:3 lines derived from cross Avocet S/Zhongmai 175 were inoculated with PST race CYR32 at the seedling stage in a greenhouse, and F2:3 lines were also evaluated for stripe rust reaction in the field using mixed PST races. BSR-seq analyses revealed 13 SNPs in the region 762.50–768.52 Mb on chromosome arm 2AL. By genome mining we identified SNPs and InDels between the parents and contrasting bulks, and mapped YrZM175 to a 0.72-cM, 636.4-kb interval spanned by YrZM175-InD1 and YrZM175-InD2 (763,452,916–764,089,317 bp) including two putative disease resistance genes based on IWGSC RefSeq v1.0. Collinearity analysis indicated similar target genomic intervals in Chinese Spring, Aegilops tauschii (2D: 647.7–650.5 Mb), Triticum urartu (2A: 750.7–752.3 Mb), Triticum dicoccoides (2A: 771.0–774.5 Mb), Triticum turgidum (2B: 784.7–788.2 Mb), and T. aestivum cv. Aikang 58 (2A: 776.3–778.9 Mb) and Jagger (2A: 789.3–791.7 Mb). Through collinearity analysis, sequence alignments of resistant and susceptible parents and gene expression level analysis, we predicted TRITD2Bv1G264480 from Triticum turgidum to be a candidate gene for map-based cloning of YrZM175. A gene-specific marker for TRITD2Bv1G264480 co-segregated with the resistance gene. Molecular marker analysis and stripe rust response data revealed that YrZM175 was different from genes Yr1, Yr17, Yr32 and YrJ22 located on chromosome 2A. Fine mapping of YrZM175 lays a solid foundation for functional gene analysis and marker-assisted selection for improved stripe rust resistance in wheat.
Quality assessment and relevant clinical impact of randomized controlled trials on chronic prostatitis/chronic pelvic pain syndrome
Authors: Guo Jun , Zhao Ming , Gao Qing-He , Liu Sheng-Jing , Deng Ying-Jun , Wang Hao , Yu Wen-Xiao 
Published: April 2022

Abstract
Objective:This study evaluated the quality of randomized controlled trials (RCTs) on chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Methods:We searched PubMed, Web of Science, and Embase for RCTs (original articles) on CP/CPPS published from database establishment to 2021. The RCT quality assessment was performed using the Consolidated Standards of Reporting of Trials (CONSORT) statement and the improved Jadad scale. Results:In total, 77 RCTs were included. According to the evaluation, 26 (33.77%) papers presented the description of the specific random methods, only 6 (7.79%) papers described the allocation concealment methods, and 26 articles referred to the “blind method”. Of the RCTs, 34 (44.16%) papers recorded the number of patients who withdrew from the study, and 67 (87.01%) papers reported adverse reactions. However, few reports mentioned the sample size calculation, clinical trial registration, or information about the relevant research programs and funding. In addition, 19 (24.68%) reports had Jadad scale scores of ≥4 points, and 58 (75.32%) reports had Jadad scale scores of ≤3 points. Conclusion:To date, the quality of RCT reports on CP/CPPS has been generally poor, and the results of the RCTs should be accepted and utilized cautiously. It is suggested that researchers should follow the CONSORT statement and improved Jadad scale to standardize the design and implementation of RCTs to improve the quality of RCTs and provide reliable evidence for the treatment of CP/CPPS.
Prenatal stress perturbs fetal iron homeostasis in a sex specific manner
Authors: Zimmermann Peter , Antonelli Marta C. , Sharma Ritika , Müller Alexander , Zelgert Camilla , Fabre Bibiana , Wenzel Natasha , Wu Hau-Tieng , Frasch Martin G. , Lobmaier Silvia M. 
Published: April 2022

Abstract
The adverse effects of maternal prenatal stress (PS) on child’s neurodevelopment warrant the establishment of biomarkers that enable early interventional therapeutic strategies. We performed a prospective matched double cohort study screening 2000 pregnant women in third trimester with Cohen Perceived Stress Scale-10 (PSS-10) questionnaire; 164 participants were recruited and classified as stressed and control group (SG, CG). Fetal cord blood iron parameters were measured at birth. Transabdominal electrocardiograms-based Fetal Stress Index (FSI) was derived. We investigated sex contribution to group differences and conducted causal inference analyses to assess the total effect of PS exposure on iron homeostasis using a directed acyclic graph (DAG) approach. Differences are reported for p<0.05 unless noted otherwise. Transferrin saturation was lower in male stressed neonates. The minimum adjustment set of the DAG to estimate the total effect of PS exposure on fetal ferritin iron biomarkers consisted of maternal age and socioeconomic status: SG revealed a 15% decrease in fetal ferritin compared with CG. Mean FSI was higher among SG than among CG. FSI-based timely detection of fetuses affected by PS can support early individualized iron supplementation and neurodevelopmental follow-up to prevent long-term sequelae due to PS-exacerbated impairment of the iron homeostasis.