bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circulating cytokine-producing VP6-specific CD4+ T cells are rarely detectable in Rotarix -vaccinated Malawian children with severe rotavirus diarrhoea
Authors: Banda Chikondi Malamba- , Mhango Chimwemwe , Benedicto-Matambo Prisca , Mandolo Jonathan J. , Chinyama End , Kumwenda Orpha , Barnes Kayla G. , Cunliffe Nigel A. , Iturriza-Gomara Miren , Jambo Kondwani C. , Jere Khuzwayo C. 
Published: November 2022

Abstract
Strong CD4+ T cell-mediated immune protection following rotavirus infection has been observed in animal models, but its relevance in humans remains unclear. Here, we characterized acute and convalescent CD4+ T cell responses in Rotarix®-vaccinated children who were hospitalized with rotavirus-positive and rotavirus-negative diarrhoea in Blantyre, Malawi. Children presenting with laboratory-confirmed rotavirus infection had higher proportions of effector and central memory T helper 2 cells during acute infection i.e., at disease presentation compared to convalescence, 28 days post-infection defined by a follow-up 28 days after acute infection. However, circulating cytokine-producing (IFN-γ and/or TNF-α) rotavirus-specific VP6-specific CD4+ T cells were rarely detectable in children with rotavirus infection at both acute and convalescent stages. Moreover, following whole blood mitogenic stimulation, the responding CD4+ T cells were predominantly non-cytokine producers of IFN-γ and/or TNF-α. Our findings demonstrate limited induction of anti-viral IFN-γ and/or TNF-α-producing CD4+ T cells in rotavirus-vaccinated Malawian children following the development of laboratory-confirmed rotavirus infection.
Human OTUD6B positively regulates type I IFN antiviral innate immune responses by deubiquitinating and stabilizing IRF3
Authors: Xiong Sidong , Dong Chunsheng , Zheng Hui , Wang Jian 
Published: November 2022

Abstract
Elaborate regulation of innate immunity is necessary for the host to effectively respond to invading pathogens. As an important component of antiviral immunity transcription factors, the stability and activity of IFN regulatory factor 3 (IRF3) are tightly controlled via multiple post-translational modifications. Here, we identified a human ovarian tumor domain-containing deubiquitinase OTUD6B as a positive regulator of IRF3 that facilitates innate antiviral immune signaling. We demonstrated that OTUD6B directly hydrolyzes the lysine 33 (Lys33)-linked polyubiquitin chain at Lys315 of IRF3 by interacting with IRF3, stabilizing the protein level of IRF3, and promoting type I IFN production. Notably, OTUD6B enhanced cellular antiviral responses in vivo, as evidenced by mice that overexpressed human OTUD6B were more resistant to RNA virus infection and had reduced viral load and morbidity. These findings revealed a previously unknown role for OTUD6B in the regulation of innate antiviral immunity and may provide a potential target for enhancing host antiviral defense.
A ten-year microbiological study of Pseudomonas aeruginosa strains revealed diffusion of carbapenems and quaternary ammonium compounds resistant populations
Authors: Pottier Marine , Gravey François , Castagnet Sophie , Auzou Michel , Bénédicte Langlois , Guérin François , Giard Jean-Christophe , Léon Albertine , Hello Simon Le 
Published: November 2022

Abstract
Pseudomonas aeruginosa is one of the leading causes of healthcare-associated infections. For this study, the susceptibility profiles to antipseudomonal antibiotics and a quaternary ammonium compound, didecyldimethylammonium chloride (DDAC), widely used as a disinfectant, were established for 180 selected human and environmental hospital strains isolated between 2011 and 2020. Furthermore, a genomic study was performed to determine their resistome and clonal putative relatedness. During the ten-year study period, it was estimated that 9.5% of clinical P. aeruginosa were resistant to carbapenem, 11.9% presented an MDR profile, and 0.7% an XDR. Decreased susceptibility (DS) to DDAC was observed for 28.0% of strains that was significantly more associated with MDR and XDR profiles and from hospital environmental samples (p <0.0001). According to genomic analyses, the P. aeruginosa population unsusceptible to carbapenems and/or to DDAC was diverse but mainly belonged to top ten high-risk clones described worldwide. The carbapenem resistance appeared mainly due to the production of the VIM-2 carbapenemase (39.3%) and DS to DDAC mediated by MexAB-OprM pump efflux overexpression. This study highlights the diversity of MDR/XDR populations of P. aeruginosa which are unsusceptible to molecules that are widely used in medicine and hospital disinfection and are probably distributed in hospitals worldwide.
Signatures of VH1-69-derived hepatitis C virus neutralizing antibody precursors defined by binding to envelope glycoproteins
Authors: Capella-Pujol Joan , de Gast Marlon , Radić Laura , Zon Ian , Chumbe Ana , Koekkoek Sylvie , Olijhoek Wouter , Schinkel Janke , Gils Marit J. van , Sanders Rogier , Sliepen Kwinten 
Published: November 2022

Abstract
An effective preventive vaccine for hepatitis C virus (HCV) remains a major unmet need. Antigenic region 3 (AR3) on the E1E2 envelope glycoprotein complex overlaps with the CD81 receptor binding site and represents an important epitope for vaccine design aimed at inducing broadly neutralizing antibodies (bNAbs). Most AR3 bNAbs utilize the VH1-69 gene and share structural features that define the AR3C-class of HCV bNAbs. In this work, we identified E1E2 glycoprotein trimers that bind to the inferred VH1-69 germline precursors of AR3C-class bNAbs, providing candidates for AR3C-class germline-targeting approaches. When presented on nanoparticles these recombinant E1E2 glycoproteins efficiently activated B cells expressing inferred germline AR3C-class bNAb precursors as B cell receptors. Furthermore, we identified critical signatures in two subclasses of the AR3C-class bNAbs that will allow refined protein design. These results provide a framework for germline-targeting vaccine design strategies against HCV.
Implementation and Effectiveness of a Sustained Depression Prevention Program for High School Students in Japan
Authors: Kira Yugo , Matsumoto Misuzu , Kambara Kohei , Ogata Akiko 
Published: November 2022

Abstract
Background: High school students, particularly in Japan, suffer from severe depression. School-based cognitive-behavioral depression prevention interventions are effective countermeasures against depression among high school students. However, due to the time-consuming nature of high school curriculum, such interventions are less common among high school students than they are among elementary and junior high school students, making it difficult to introduce them to a sustainable school curriculum. We used a long-term but low-frequency intervention as one of the implementation approaches to address curricular issues, which divide the program’s content across several grades. Therefore, this study examined the effectiveness of our approach to high school students.
Methods: We conducted the program on a grade-by-grade basis for students who entered the school each year over a two-year period. We administered surveys during the two-year intervention period and one-year follow-up period to 225 students who had enrolled in a high school during 2014–2019. Only those high schools were considered that implemented our program. Additionally, data from 608 Japanese high school students who did not receive this program were obtained through an online survey and compared to our data for higher levels of depressive symptoms at each grade level.
Results: According to the three-level hierarchical linear model analysis, students who participated in the program experienced improvement in their depressive symptoms and related factors over these three years. At the end of the program and one year after the intervention, the Welch test and chi-square test indicated fewer depressive symptoms and a lower percentage of students with depression compared to those who did not receive the intervention.
Conclusions: The results of this study suggest that a school-based cognitive-behavioral depression prevention program using a long-term but low-frequency approach may effectively reduce the implementation burden into the school curriculum, promote sustained implementation, and prevent depression among high school students. Future studies may consider other innovative methods to examine sustained intervention effects, considering economic and ethical issues, such as collecting depression scores of general high school students annually.
Predisposing factors, direction of dislocation, and changes in intraocular pressure after intrascleral intraocular lens fixation surgery
Authors: Saito Yuya , Shimizu Norihiro , Mashimo Yoichi , Tatsumi Tomoaki , Yokouchi Hirotaka , Baba Takayuki 
Published: November 2022

AbstractThe purpose of the present study is to identify predisposing factors and direction of dislocation of the natural crystallinelens or intraocular lens (IOL) in IOL intrascleral fixation surgery and determine changes in intraocular pressure (IOP) after surgery. We retrospectively investigated predisposing factors, direction of the lens, IOL dislocation, and IOP preoperatively and 1 day, 1 week, and 1 month postoperatively in 240 eyes from 232 patients who underwent IOL intrascleral fixation. The surgery was performed in 48 patients with long eye axis, 44 with pseudoexfoliation (PE), 42 with intraoperative problems, 40 with a history of trauma, 34 with a history of vitrectomy, 21 with atopic dermatitis, 6 with genetic diseases, 4 with retinitis pigmentosa, and 56 with unknown causes. Downward IOL dislocation was most observed (52 cases), while IOL falling into the vitreous cavity was seen in 46 cases, aphakic eye in 31 cases, and anterior lens dislocation in 16 cases. In general, IOP was significantly lower at 1 month postoperatively than preoperatively; specifically, in the PE, history of trauma, atopic dermatitis, long eye axis, and unknown cause groups, as well as the groups with anterior lens deviation, IOL fall into the vitreous cavity, and IOL downward deviation.
The diagnostic accuracy of Serum microRNAs in detection of cervical cancer: A systematic review protocol
Authors: Ssedyabane Frank , Niyonzima Nixon , Ngonzi Joseph , Tusubira Deusdedit , Ocan Moses , Akena Dickens , Namisango Eve , Apunyo Robert , Kinegyere Alison Annet , Obuku Ekwaro A 
Published: November 2022

Abstract
Background: Cervical cancer remains a public health problem worldwide especially sub-Saharan Africa. There are challenges in timely screening and diagnosis for early detection and intervention. Therefore, studies on cervical cancer and cervical intraepithelial neoplasia suggest the need for new diagnostic approaches including microRNA technology. Plasma/serum levels of micro RNAs are elevated or reduced compared to the normal state and their diagnostic accuracy for detection of cervical neoplasms has not been rigorously assessed more so in low resource settings such as Uganda. The aim of this systematic review was therefore to assess the diagnostic accuracy of serum microRNAs in detecting cervical cancer.
Methods: We performed a systematic review following the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) statement. We searched for all English articles in MEDLINE/PubMed, Web of Science, Embase, CINAHL, as well as grey literature through 2022. Our outcomes were sensitivity, specificity, negative predictive values, positive predictive values or area under the curve (1) for each microRNA. We used the quality assessment of diagnostic accuracy studies (2) tool to assess risk of bias of included studies. Our results are reported according to the Preferred Reporting Items for Systematic Reviews and Meta-analysis for Diagnostic Test Accuracy studies (PRISMA-DTA)
Use of the review results: This systematic review will provide information on the relevance of microRNAs in cervical cancer. This information will help policy makers, planners and researchers in determining which particular microRNAs could be employed to screen, diagnose or monitor prognosis of cancer of the cervix.
Systematic review registration: This protocol has been registered in PROSPERO under registration number CRD42022313275.
Computational Radical-Scavenging Activity Investigation of Galantamine
Authors: Karadjova Veronika , Saso Luciano , Velkov Zhivko 
Published: November 2022

Abstract
Galantamine is a natural alkaloid witha proved antioxidant activity, which easily passes the blood-brain barrier. This makes it a reliable drug for the treatment of many human brain diseases.In this paper we report the results of quantum chemical calculations of the dissociationenthalpies of O-H and C-H bonds in galantamine. The results clearly show the propensity of galantamine to react with radicals in the living organisms.This includes an assessment of the stability of O-H and C-H bonds in it, as well as their acidity.In addition, hydride ion abstraction reactions such as occur with nicotine-amid-adenine-dinucleotide (NAD) are discussed here.
Parameters optimization of a nonlinear dynamic absorber for a nonlinear system
Authors: Nazari Mohammad Mahdi , Rahi Abbas 
Published: November 2022

Abstract
One of the important methods to prevent the intensification of the main vibration system is using a dynamic vibration absorber (DVA). A DVA is a mechanical subsystem used to reduce Vibration amplitude and prevent the main vibration system from resonance. This paper aims to use a nonlinear dynamic absorber as a cantilever beam with a concentrated mass at its end to reduce the amplitude of the intensified oscillator vibration. The primary vibration system consists of mass, nonlinear spring, and viscous damping, and a harmonic excitation force is applied to it, resonance the system. In this case, using a nonlinear dynamic absorber reduces the vibration amplitude. For this purpose, first, the kinetic energy and the potential energy of the vibration system are calculated, and then, using Lagrange equations, the governing equations are extracted. Due to the nonlinearity of the equations of motion, the multiple scales method is used to solve the governing equations. In the results, the effect of nonlinear DVA on reducing the amplitude of intensified oscillator vibration is well visible. The effect of the main parameters of nonlinear dynamic absorbers on the vibration amplitude of the initial system is also investigated. Finally, the vibration amplitude of the system is optimized as a target function by the genetic algorithm method, and the optimal parameters of the nonlinear dynamic absorber and the optimal vibration amplitude are studied. Considering the main resonant vibration system as a structure under earthquake, the results of this research in reducing the vibration amplitude can be widely used in earthquake engineering.
Risk factors for perioperative acute heart failure in elderly hip fracture patients and establishment of a nomogram predictive model
Authors: Tian Miao , Zhu Yanbin , Li Wenjing , Wang Yan , Tian Yunxu , Zhang Kexin , Li Xiuting 
Published: November 2022

Abstract
Objective
This study aims to explore the risk factors of perioperative acute heart failure in elderly patients with hip fractures and establish a nomogram prediction model.
Methods
The present study was designed as retrospective. From January 2020 to December 2021, patients who underwent surgical treatment for hip fractures at the Third Hospital of Hebei Medical University were included. Patients with heart failure were confirmed by discharge diagnosis or medical records. Samples were randomly divided into modeling and validation cohorts at a ratio of 7:3. The demographic and relevant clinic data of patients were collected. Univariate and multivariate logistic regression analyses were conducted by IBM SPSS Statistics 26.0, to obtain the risk factors of acute heart failure. R software was used to construct the nomogram prediction model.
Results
A total of 751 elderly patients with hip fractures were enrolled in this study, of which 138 patients (18.37%, 138/751) developed acute heart failure. Patients with heart failure were confirmed by discharge diagnosis or medical records. Respiratory disease (odd ratio, 7.135; 95% confidence interval, 3.615–14.081; P value, 0.001), cardiovascular disease (odd ratio, 2.250, 95% confidence interval, 1.214–4.170; P value, 0.010), ASA ≥ 3 (odd ratio, 13.733, 95% confidence interval, 7.465–25.265; P value, 0.001), and preoperative waiting time ≤ 2 days (odd ratio, 3.271, 95% confidence interval, 1.315–8.134; P value, 0.011) were the independent risk factors of perioperative acute heart failure in elderly patients with hip fracture. The area under the curve (AUC) of the prediction model based on the above factors was calculated to be 0.877 (95% confidence interval 0.836–0.918). The sensitivity and specificity were 82.8% and 80.9% respectively, and the fitting degree of the model was good. In the internal validation group, the area under the curve (AUC) was 0.910, the 95% confidence interval was 0.869–0.950.
Conclusions
Our nomogram model can be used to predict the risk of acute heart failure in elderly hip fractures during the perioperative period and provide guidance on the prevention of acute heart failure.
Surgical treatment of double aortic arch malformation combined with descending aortic arch dissection in adults: a case series
Authors: Min Xinping , Hu Zhipeng , Wang Zhiwei , Xia Jun 
Published: November 2022

Abstract
Background: Double aortic arch (DAA) combined with descending aortic arch dissection (DAAD) in adults is a rare aorta vascular disease. Due to the abnormal anatomy of the double arch and arch vessels, the clinical symptoms and surgical methods differ from those of typical aortic dissection.
Methods: This study was retrospective analysis of a case series involving three patients (mean age, 47.3 years) with DAA combined DAAD underwent total arch replacement or hybrid aortic repair from September 2010 to June 2019. The patients’ demographics, initial symptoms, comorbidities, surgical procedures, and outcomes are summarized.
Results: Total arch replacement plus frozen stent implantation under deep hypothermic circulatory arrest was performed for 2 patients，one of them developed disseminated intravascular coagulation and multiple organ failure postoperatively. Case 3 underwent a hybrid procedure with left subclavian artery revascularization and thoracic endovascular aortic repair. The symptoms of hoarseness and dysphagia were obviously improved during the follow up.
Conclusion: In addition to typical sudden chest and back pain, patients with DAA and DAAD may have hoarseness and dysphagia. Based on the development of DAA, total arch replacement or hybrid surgery may be is an optional treatment.
Comparing the effectiveness of metformin with lifestyle modification for the primary prevention of type Ⅱ diabetes: A systematic review and meta-analysis
Authors: Mousavi Seyedeh Sarah , Namayandeh Seyedeh Mahdieh , Fallahzadeh Hossein , Rahmanian Masoud , Mollahosseini Mehdi 
Published: November 2022

Abstract
Background: concerning ascending trend in prevalence of chronic type Ⅱ diabetes, prevention and the development of an effective approach after recognition of at risk individuals are crucial. The aim of this study is to investigate the influence of lifestyle modification and metformin interventions in the prevention of type Ⅱ diabetes developments.
Method: The search was conducted using PubMed, Google scholar, Scopus and Web of science data bases. The inclusion criteria include randomized controlled trials (RCT) which studied both lifestyle modification and metformin interventions in the population above 18 years old without history of any types of diabetes. After excluding studies with intervention time less than 6 months, systematic review and meta-analysis were performed to evaluate relative risk (RR) with confidence interval (CI) 95% of type Ⅱ diabetes development.
Results: data from 5 studies were included in meta-analysis. The population also consists of individuals with mean age of 50 years old with BMI and FBS of 35.5 and 104.7 mg/dl respectively. Participants’ range of prevention year was between 2-3 years with mean of 2.8 years. Lifestyle modification decreases the probability of the incidence of type Ⅱ diabetes by 25.3 % (RR: 0.747, 95% CI, 0.6 - 0.92) compared to the metformin intervention (p-value= 0.007). Our results indicate that long-term lifestyle modifications can prevent diabetes type Ⅱ and decrease diabetes mellitus incidence down to one-quarter in comparison to metformin.
Conclusion: lifestyle modification can be more efficacious than metformin in diminishing incidence of type Ⅱ diabetes. Therefore, lifestyle modification can be a therapeutic strategy for controlling type Ⅱ diabetes incidence especially in high risk individuals.
Preoperative Ultrasound-based Protocol for Optimisation of Fluid Therapy to Prevent Early Intraoperative Hypotension: A Randomised Controlled Study
Authors: Szabó Marcell , Pleck András , Soós Sándor Árpád , Keczer Bánk , Varga Balázs , Széll János 
Published: November 2022

Abstract
Purpose: We aimed to evaluate whether the implication of an ultrasound-based preoperative fluid therapy protocol can decrease the incidence of early intraoperative hypotension.
Methods: Randomised controlled study in a tertiary university department involving elective surgical patients of ASA 2-3 class, scheduled for elective major abdominal surgery under general anaesthesia with intubation. We randomized 40–40 patients; 38–38 were available for analysis. Conventional fluid therapy was ordered on routine preoperative visits. Ultrasound-based protocol evaluated the collapsibility index of inferior vena cava and lung ultrasound profiles. Scans were performed twice: 2 h and 30 min before surgery. A high collapsibility index (≥40%) indicated a standardised fluid bolus, while the anterior B-profile of the lung ultrasound contraindicated further fluid. The primary outcome was the incidence of postinduction and early intraoperative (0–10 minutes) hypotension (MAP<65 mmHg and/or ≥30% of decrease from baseline). Secondary endpoints were postoperative lactate level, urine output and lung ultrasound score at 24 h.
Results: The absolute criterion of postinduction hypotension was fulfilled in 12 patients in the conventional group (31.6%) and 3 in the ultrasound-based group (7.9%) (p=0.0246). Based on composite criteria, we observed 17 (44.7%) and 7 (18.4%) cases, respectively (p=0.0136). The incidence of early intraoperative hypotension was also lower: HR for absolute hypotension was 2.10 (95%CI 1.00–4.42) in the conventional group (p=0.0387). Secondary outcome measures were similar in the study groups.
Conclusion: We implemented a safe and effective point-of-care ultrasound-based preoperative fluid replacement protocol into perioperative care.
Trial registration: Clinicaltrials.gov, NCT05171608 (registered prospectively on 10/12/2021)