bluuebunny/crossref_metadata_2025_split huggingface dataset:
Key considerations when involving children in health intervention design: reflections on working in partnership with South Asian children in the UK on a tailored Management and Intervention for Asthma (MIA) study
Authors: Nixon Laura S. , Hudson Nicky , Culley Lorraine , Lakhanpaul Maya , Robertson Noelle , Johnson Mark R. D. , McFeeters Melanie , Johal Narynder , Hamlyn-Williams Charlotte , Boo Yebeen Ysabelle , Lakhanpaul Monica 
Published: February 2022

AbstractParticipatory research is an empowering process through which individuals can increase control over their lives, and allows researchers/clinicians to gain a clearer understanding of a child’s needs. However, involving children in participatory research is still relatively novel, despite national and international mandates to engage children in decision making. This paper draws on the learnings from designing the Management and Intervention for Asthma (MIA) study, which used a collaborative participatory method to develop an intervention-planning framework for South Asian children with asthma. There are currently 1 million children in the UK receiving treatment for asthma, making it one of the most prevalent chronic childhood illnesses. Symptoms of asthma are often underrecognized in children from South Asian communities in the UK, contributing to increased disease severity and increased attendance at the emergency department compared to White British children. Despite this, ethnic minorities are often excluded from research and thus absent from the ‘evidence base’, making it essential to hear their perspectives if health inequalities are to be successfully addressed. We worked alongside healthcare professionals, community facilitators, parents, and children to identify the key concerns and priorities they had and then designed the framework around their needs. Reflecting on the process, we identified several key considerations that need to be addressed when co-developing interventions with children. These include the power dynamics between the parent/researcher and child; navigating the consent/assent process; how parental involvement might affect the research; establishing a convenient time and location; how to keep children engaged throughout the process; tailoring activities to different levels of ability; and accounting for cultural differences. These factors were considered by the researchers when designing the study, however, implementing them was not without its challenges and highlighted the need for researchers to develop expertise in this field. Tailoring existing research methods allowed us to explore children’s perceptions, priorities, and experiences of illness more effectively. However, involving children in participatory research is a complex undertaking, and researchers need to ensure that they have the expertise, time, and resources necessary to be able to fully support the needs of child participants before deciding to commit to this approach.
Optical coherence tomography in diagnosing polypoidal choroidal vasculopathy. Looking into the future: a systematic review and meta-analysis
Authors: Permadi Annisa C. , Djatikusumo Ari , Adriono Gitalisa Andayani 
Published: February 2022

Abstract
Background
Polypoidal choroidal vasculopathy (PCV) is an exudative maculopathy with features similar to wet age macular degeneration. The incidence of PCV is known to be higher in the Asian population compared to Caucasians. Imaging modality is needed to make the diagnosis of PCV. Although Indocyanine green angiography (ICGA) is still the gold standard, it is not routinely performed in vitreoretinal practice. Thus another imaging modality is currently a popular research area. Spectral domain optical coherence tomography (SD-OCT) has emerged as a new imaging modality mostly available in clinics. Some studies have reported the sensitivity and specificity of SD-OCT in diagnosing PCV with different results and thresholds.

Methods
Relevant studies from PubMed, Science Direct and Google Scholar databases were systematically searched. In random effect models using STATA 14 software, a meta-analysis was performed to determine the pooled diagnostic accuracy. QUADAS 2 was used to evaluate the risk of bias of each study by Revman 5.4 software.

Results
Seven eligible studies which met the inclusion and exclusion criteria were enrolled in this study. A total of 911 eyes were included to investigate the diagnostic accuracy of SD-OCT. As a result, the pooled sensitivity was 0.91 (95% CI 0.87–0.93), specificity 0.88 (95% 0.83–0.92), positive likelihood ratio 8, negative likelihood ratio 11, the area under the summary receiver operating characteristic curve 0.95 (95% CI 0.93–0.97), and diagnostic odds ratio 71.81 (95% CI 38.89–132.74).

Conclusion
SD-OCT provided a high diagnostic value for detecting PCV. Sharply peaked pigment epithelial detachment (PED), notched PED, bubble sign, multiple PED, and double-layer sign were the most common features found in PCV.
Terahertz nano-imaging of metal-insulator transition in Cd2Os2O7
Authors: Jing Ran , Xiong Lin , Hirose Hishiro T. , Shao Yinming , Mcleod Alex S. , Chen Xinzhong , Liu Mengkun , Hiroi Zenji , Basov D. N. 
Published: February 2022

Abstract
The osmate pyrochlore Cd2Os2O7 supports an antiferromagnet insulator ground state with an all-in/all-out (AIAO) spin ordering at low temperature. Above 225 K, Cd2Os2O7 becomes a paramagnetic metal whereas the mechanism of this metal-to-insulator transition (MIT) remains elusive. In this letter, we use cryogenic near-field technique operating at terahertz frequencies to study the evolution of low-energy response across the MIT. We observed a systematic variation of the magnitude of nano-THz signal across the transition, consistent with the trend in the direct-current conductivity. Conducting domain walls that dominate the nano-scale landscape of the conductivity of a closely related AIAO system Nd2Ir2O7 are not apparent in Cd2Os2O7.
Heart rate as a proxy for estimating oxygen consumption rates in loggerhead turtles (Caretta caretta)
Authors: Kinoshita Chihiro , Saito Ayaka , Sakamoto Kentaro Q. , Niizuma Yasuaki , Sato Katsufumi 
Published: February 2022

ABSTRACT
Heart rates of air-breathing diving animals can change on a short time scale due to the diving response during submergence. Heart rate is used frequently as a proxy for indirectly estimating metabolic rates on a fine time scale. However, most studies to date have been conducted on endothermic diving animals, and the relationships between metabolic rates and heart rates in ectothermic diving animals have not been well studied. Sea turtles are unique model organisms of diving ectotherms because they spend most of their life in the ocean and perform deep and/or long dives. In this study, we examined the relationship between heart rates and metabolic rates in captive loggerhead turtles, Caretta caretta, to estimate oxygen consumption rates during each dive based on heart rates. The oxygen consumption rates (V̇O2: mlO2 min−1 kg−1) and average heart rates (fH: beats min−1) were measured simultaneously in indoor tanks at water temperatures of 15–25°C. Our results showed that oxygen consumption rate was affected by heart rate and water temperature in loggerhead turtles. Based on the collected data, we formulated the model equation as V̇O2=0.0124fH+0.0047Tw - 0.0791. The equation can be used for estimating fine-scaled field metabolic rates in free-ranging loggerhead turtles. The results of this study will contribute to future comparative studies of the physiological states of ectothermic diving animals.
Single-cell RNA sequencing reveals PDGFRα+ stromal cell subpopulations that promote proacinar cell differentiation in embryonic salivary gland organoids
Authors: Moskwa Nicholas , Mahmood Ayma , Nelson Deirdre A. , Altrieth Amber L. , Forni Paolo E. , Larsen Melinda 
Published: February 2022

ABSTRACT
Stromal cells can direct the differentiation of epithelial progenitor cells during organ development. Fibroblast growth factor (FGF) signaling is essential for submandibular salivary gland development. Through stromal fibroblast cells, FGF2 can indirectly regulate proacinar cell differentiation in organoids, but the mechanisms are not understood. We performed single-cell RNA-sequencing and identified multiple stromal cell subsets, including Pdgfra+ stromal subsets expressing both Fgf2 and Fgf10. When combined with epithelial progenitor cells in organoids, magnetic-activated cell-sorted PDGFRα+ cells promoted proacinar cell differentiation similarly to total stroma. Gene expression analysis revealed that FGF2 increased the expression of multiple stromal genes, including Bmp2 and Bmp7. Both BMP2 and BMP7 synergized with FGF2, stimulating proacinar cell differentiation but not branching. However, stromal cells grown without FGF2 did not support proacinar organoid differentiation and instead differentiated into myofibroblasts. In organoids, TGFβ1 treatment stimulated myofibroblast differentiation and inhibited the proacinar cell differentiation of epithelial progenitor cells. Conversely, FGF2 reversed the effects of TGFβ1. We also demonstrated that adult salivary stromal cells were FGF2 responsive and could promote proacinar cell differentiation. These FGF2 signaling pathways may have applications in future regenerative therapies.
Requirement of phosphatidic acid binding for distribution of the bacterial protein Lpg1137 targeting syntaxin 17
Authors: Murata Misaki , Kanamori Riku , Kitao Tomoe , Kubori Tomoko , Nagai Hiroki , Tagaya Mitsuo , Arasaki Kohei 
Published: February 2022

ABSTRACT
The gram-negative bacterium, Legionella pneumophila is known to manipulate the host cellular functions. L. pneumophila secretes bacterial proteins called Legionella effectors into the host cytosol that are necessary for these manipulations. The Legionella effector Lpg1137 was identified as a serine protease responsible for the degradation of syntaxin 17 (Stx17). However, how Lpg1137 specifically recognizes and degrades Stx17 remained unknown. Given that Stx17 is localized in the ER, mitochondria-associated membrane (MAM), and mitochondria, Lpg1137 likely distributes to these compartments to recognize Stx17. Here, we show that the C-terminal region of Lpg1137 binds to phosphatidic acid (PA), a MAM and mitochondria-enriched phospholipid, and that this binding is required for the correct intracellular distribution of Lpg1137. Two basic residues in the C-terminal region of Lpg1137 are required for PA binding and their mutation causes mislocalization of Lpg1137. This mutant also fails to degrade Stx17 while retaining protease activity. Taken together, our data reveal that Lpg1137 utilizes PA for its distribution to the membranous compartments in which Stx17 is localized.
Colour vision in stomatopod crustaceans: more questions than answers
Authors: Streets Amy , England Hayley , Marshall Justin 
Published: February 2022

ABSTRACT
Stomatopod crustaceans, or mantis shrimps, are known for their extensive range of spectral sensitivity but relatively poor spectral discrimination. Instead of the colour-opponent mechanism of other colour vision systems, the 12 narrow-band colour channels they possess may underlie a different method of colour processing. We investigated one hypothesis in which the photoreceptors are proposed to act as individual wave-band detectors, interpreting colour as a parallel pattern of photoreceptor activation, rather than a ratiometric comparison of individual signals. This different form of colour detection has been used to explain previous behavioural tests in which low-saturation blue was not discriminated from grey, potentially because of similar activation patterns. Results here, however, indicate that the stomatopod Haptosquilla trispinosa was able to easily distinguish several colours, including blue of both high and low saturation, from greys. The animals did show a decrease in performance over time in an artificially lit environment, indicating plasticity in colour discrimination ability. This rapid plasticity, most likely the result of a change in opsin (visual pigment) expression, has now been noted in several animal lineages (both invertebrate and vertebrate) and is a factor we suggest needs attention and potential re-examination in any colour-based behavioural tests. As for stomatopods, it remains unclear why they achieve poor colour discrimination using the most comprehensive set of spectral sensitivities in the animal kingdom and also what form of colour processing they may utilise.
Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter metabolomics signature
Authors: Cheredath Aswathi , Uppangala Shubhashree , Asampille Gitanjali , Lakshmi R. Vani , Joseph David , Raval Keyur , Gowda G. A. Nagana , Kalthur Guruprasad , Adiga Satish Kumar 
Published: February 2022

Background: The extended embryo culture using single-step medium gained popularity in clinical in vitro fertilisation (IVF). However, there are concerns about the degradation of unstable medium components and their negative effects on the developing embryos. Further, dry-incubation can increase osmolality, which can in-turn enhance the concentration of constituents of the media and their stability. Hence, this study was conducted to understand the immediate changes in the culture media metabolites in relation to clinically comparable situations such as single-step extended embryo culture and use of dry and humidified-incubation in two-different gaseous conditions. Methods: Commercially available single-step medium was sham-cultured in droplets under oil in two different conditions viz. dry (37°C; 6%CO2; 5%O2) and humidified (37°C; 6% CO2; atmospheric O2) for 0h, 72h, and 120h intervals. Droplets were subjected to the sensitivity-enhanced nuclear magnetic resonance (NMR)-based profiling using 800 MHz NMR equipped with a cryogenically cooled micro-coil (1.7mm) probe. Metabolomic signatures between the two groups were comprehensively assessed. Results: A total of ten amino acids and four energy substrates were identified from the culture medium. Metabolite levels showed a non-significant increase in the dry-incubation group at 72h and then declined at 120h. Humidified incubation had no effects on the level of the metabolite until 120h. No significant differences in the levels of metabolites were observed between the dry and humidified-groups at various time-points tested. Conclusions: A non-significant variation in the levels of metabolites observed in the dry-incubation of single-step medium most unlikely to influence a clinical outcome. However, the impact of these subtle changes on the (epi)genetic integrity of the embryos in a clinical set-up to be addressed.
The role of newspapers published in North Sumatra during Indonesia's independence struggle between 1916-1925: A discourse analysis
Authors: Azhari Ichwan , Sidiq Ricu , Purnamasari Ika 
Published: February 2022

Background: In the history of Indonesian independence, newspapers not only functioned as a medium of information but also as a tool for the struggle for independence. This is because of the role of newspapers in bringing out the spirit of nationalism and the concept of “nation” to the Indonesian people through various writings and reports in each edition. This study aims to describe the form of Indonesia's struggle for independence in newspapers published in North Sumatra in 1916-1925. Methods: This research uses the critical discourse analysis method. The research data are in the form of texts published in thirteen indigenous newspapers published in North Sumatra in 1916-1925. Data analysis used three structures of Teun A. van Dijk's discourse analysis model, namely: macrostructure, superstructure, and microstructure. Results: From 1910-1925 there were 24 indigenous newspapers published in North Sumatra. Of these, there were 13 newspapers demanding Indonesian independence: Soeara Djawa, Pewarta Deli, Benih Merdeka, Perempoean Bergerak, Soeara Bondjol, Sinar Zaman, Orgaan Bataksche Studiefonds, Andalas, Mandailing, Warta Timur, Al Moektabas, Tjermin Karo, Soeara Batak. These newspapers published articles that fought for Indonesian independence by demanding Indonesian independence openly, criticizing various policies of the Dutch Colonial Government, and building awareness of Indonesian nationalism. Conclusion: The role of newspapers in the struggle for Indonesian independence in North Sumatra in 1916-1925 can be seen from the findings of 51 articles demanding Indonesia's independence from Dutch colonialism, criticizing the policies of the Dutch colonial government, and building the spirit of nationalism to encourage the Indonesian people to fight for their independence.
Modified recurrent equation-based cubic spline interpolation for missing data recovery in phasor measurement unit (PMU)
Authors: Thangaraj Shruthi , Goh Vik Tor , Yap Timothy Tzen Vun 
Published: February 2022

Background: Smart grid systems require high-quality phasor measurement unit (PMU) data for proper operation, control, and decision-making. Missing PMU data may lead to improper actions or even blackouts. While the conventional cubic interpolation methods based on the solution of a set of linear equations to solve for the cubic spline coefficients have been applied by many researchers for interpolation of missing data, the computational complexity increases non-linearly with increasing data size. Methods: In this work, a modified recurrent equation-based cubic spline interpolation procedure for recovering missing PMU data is proposed. The recurrent equation-based method makes the computations of spline constants simpler. Using PMU data from the State Load Despatch Center (SLDC) in Madhya Pradesh, India, a comparison of the root mean square error (RMSE) values and time of calculation (ToC) is calculated for both methods. Results: The modified recurrent relation method could retrieve missing values 10 times faster when compared to the conventional cubic interpolation method based on the solution of a set of linear equations. The RMSE values have shown the proposed method is effective even for special cases of missing values (edges, continuous missing values). Conclusions: The proposed method can retrieve any number of missing values at any location using observed data with a minimal number of calculations.
A comparison of the population pharmacokinetics of rifampicin, isoniazid and pyrazinamide between hospitalized and non-hospitalized tuberculosis patients with or without HIV
Authors: Abdelgawad Noha , Chirehwa Maxwell , Schutz Charlotte , Barr David , Ward Amy , Janssen Saskia , Burton Rosie , Wilkinson Robert J. , Shey Muki , Wiesner Lubbe , McIlleron Helen , Maartens Gary , Meintjes Graeme , Denti Paolo 
Published: February 2022

Background. Early mortality among hospitalized HIV-associated tuberculosis (TB/HIV) patients is high despite treatment. The pharmacokinetics of rifampicin, isoniazid, and pyrazinamide were investigated in hospitalized TB/HIV patients and a cohort of outpatients with TB (with or without HIV) to determine whether drug exposures differed between groups. Methods. Standard first-line TB treatment was given daily as per national guidelines, which consisted of oral 4-drug fixed-dose combination tablets containing 150 mg rifampicin, 75 mg isoniazid, 400 mg pyrazinamide, and 275 mg ethambutol. Plasma samples were drawn on the 3rd day of treatment over eight hours post-dose. Rifampicin, isoniazid, and pyrazinamide in plasma were quantified and NONMEM® was used to analyze the data. Results. Data from 60 hospitalized patients (11 of whom died within 12 weeks of starting treatment) and 48 outpatients were available. Median (range) weight and age were 56 (35 - 88) kg, and 37 (19 - 77) years, respectively. Bioavailability and clearance of the three drugs were similar between TB/HIV hospitalized and TB outpatients. However, rifampicin’s absorption was slower in hospitalized patients than in outpatients; mean absorption time was 49.9% and 154% more in hospitalized survivors and hospitalized deaths, respectively, than in outpatients. Higher levels of conjugated bilirubin correlated with lower rifampicin clearance. Isoniazid’s clearance estimates were 25.5 L/h for fast metabolizers and 9.76 L/h for slow metabolizers. Pyrazinamide’s clearance was more variable among hospitalized patients. The variability in clearance among patients was 1.70 and 3.56 times more for hospitalized survivors and hospitalized deaths, respectively, than outpatients. Conclusion. We showed that the pharmacokinetics of first-line TB drugs are not substantially different between hospitalized TB/HIV patients and TB (with or without HIV) outpatients. Hospitalized patients do not seem to be underexposed compared to their outpatient counterparts.
Picosecond laser microwelding of AlSi–YAG for laser system assembly
Authors: Hann Samuel N. , Sanwell Jake , Carter Richard M. , Elder Ian , Lamb Robert , Esser M. J. Daniel , Hand Duncan P. 
Published: February 2022

We report the successful picosecond laser welding of AlSi and YAG. This material combination is of significant interest to the field of laser design and construction. Parameter maps are presented that demonstrate the impact of pulse energy and focal position on the resultant weld. Weld performance relevant to industrial applications is measured, i.e., shear strength, process yield, and absolute thermal resistance are presented.
High-precision four-dimensional hyperspectral imager integrating fluorescence spectral detection and 3D surface shape measurement
Authors: Luo Jing , Forsberg Erik , Fu Shuai , He Sailing 
Published: February 2022

A four-dimensional hyperspectral imager (FDHI) that combines fluorescence spectral detection and 3D surface morphology measurement is proposed. The FDHI consists of a hyperspectral line-scanner, a line structured light stereo vision system, and a line laser. The line laser is used as both the excitation light for the fluorescence and the scanning light line for the 3D profiling. At each scanning step, the system collects both fluorescent and 3D spatial data of the irradiated line region, which are fused to 4D data points based on a line mapping relationship between the datasets, and by scanning across the measurement object, a complete 4D dataset is obtained. The FDHI shows excellent performance with spatial and spectral resolution of 26.0 µm and 3 nm, respectively. The reported FDHI system and its applications provide a solution for 4D detection and analysis of fluorescent objects in meters measurement range, with advantage of high integration as two imaging modules sharing a same laser source.