bluuebunny/crossref_metadata_2025_split huggingface dataset:
New trends in the development of local self-government in the light of the 2020 constitutional reform
Authors: Чихладзе Л.Т. , Ганина О.Ю. 
Published: August 2022

В статье анализируются актуальные вопросы развития системы органов местного самоуправления в свете конституционной реформы 2020 г. Исследовано понятие «местное самоуправление» по Федеральному закону «Об общих принципах организации местного самоуправления в Российской Федерации» и законопроекту о местном самоуправлении. Затронута проблема функционирования органов местного самоуправления в условиях укрупнения муниципальных образований и перехода к одноуровневой системе организации местного самоуправления в Российской Федерации.
New trends in the development of local self-government in the light of the 2020 constitutional reformAnnotation: The article analyzes topical issues of the development of the system of local governments in the light of the constitutional reform of 2020. The concept of "local selfgovernment" according to the Federal Law "On the General Principles of Organization of Local Self-Government in the Russian Federation" and the draft law on local self-government is studied. The problem of the functioning of local governments in the context of consolidation of municipalities and the transition to a single-level system of organization of local government in the Russian Federation is touched upon.
Tax reconstruction is a new increment of tax legal relations.
Authors: Фархутдинов Р.Д. 
Published: August 2022

Актуальность вопроса применения в налоговых правоотношениях понятий и дефиницией, отсутствующих в праве, предопределяет необходимость исследования такого научного феномена. Важным и практически необходимым полагаем определить регуляторы, позволяющие защитить частные интересы сторон в договоре, выявив пределы воздействия публичного права в коммерческом договоре.
The relevance of the issue of the application of concepts and definitions that are absent in the law in tax legal relations determines the need to study such a scientific phenomenon. We consider it important and practically necessary to determine the regulators that allow protecting the private interests of the parties in the contract, and the limits of the impact of the law in the commercial contract.
Vaccination hesitancy as a cause of COVID-related mortality
Authors: Kılıç Jehat , Yıldırım M. Serdar , Alakuş Ömer Faruk , Kiliç Delyadil Karakaş , Y. Nurettin A. , Ebik Berat 
Published: August 2022

Background: In this study, our aim was to draw attention to the vaccine hesitation as a cause of unvaccination and evaluate the vaccinated and unvaccinated patients in the intensive care unit in terms of morbidity and mortality.Methods: A hundred and ninety-nine patients between the May 2021-September 2021 admitted to the intensive care units in our hospital were included in this study. We included the patients with COVID-19 infection as assessed by reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay.Results: Despite the availability of eligible vaccines in our region, the most of included patients in this study were unvaccinated (n:119). Eighty patients were vaccinated. The vaccinated patients were divided into six groups according to their vaccination status such as subgroup 1: one-dose Coronavac, subgroup 2: two-doses Coronavac, subgroup 3: three-doses Coronavac, subgroup 4: 2-doses Coronavac+ One-dose BNT162b2, subgroup 5: one-dose BNT162b2, subgroup 6: two-doses BNT162b2 (Numbers of patients: 7-61-3-1-10-0 respectively for each group). Between the vaccinated and unvaccinated groups, there was no significant difference in terms of mortality (p>0,05). The majority of patients in the vaccinated group were the patients vaccinated with Coronavac (subgroup 2:59), and there was no full dose vaccinated patient with BNT162b2.Conclusions: While both vaccines are effective to reduce the morbidities and mortality related to COVID-19, there might not be efficacy in these vaccines, particularly Coronavac, following the admission to the intensive care unite in the more vulnerable population. And despite the availability of vaccines, vaccine-hesitancy may be one of the significant reasons for the hospitalization process.
A rare case of paraneoplastic neurological syndrome with ovarian teratoma
Authors: Kumar Anita , Dabral Anjali , Panwar Megha , Bajaj Bindu , Sanapala Sukanya , Sharma Deepti 
Published: August 2022

Paraneoplastic neurological syndrome (PNS) is a neuropathological disorder of central nervous system, in this the patient presents with a tumor anywhere in the body with diverse neuropsychiatric symptoms. The clinical manifestation of the tumor may be preceded by PNS. We are reporting a case of a 23-year-old married nulliparous female who came to the gynaecology outpatient department (OPD) with the history of mass per abdomen for the past four months and also undergoing treatment for acute psychosis, memory loss and cognitive impairment for the past five months. Her symptoms were not relieved on treatment. After clinical, laboratory and radiological evaluation possibility of paraneoplastic neurological syndrome associated with ovarian teratoma was made. Patient was taken up for staging laparotomy with U/L salpingoophrectomy. After surgical treatment her psychiatric symptoms rapidly improved and her psychiatric medications doses was reduced. PNS should be suspected in female patients presenting with an acute history of neuropsychiatric symptoms. Tumor resection should be performed at the earliest to improve patient outcomes.
O Cuidado em Saúde em uma Unidade de Cuidados Continuados Integrados em Tempos de Covid-19
Authors: Silva Gabriela Thaís da 
Published: August 2022

Trata-se de um estudo observacional do tipo relato de experiência. Tem como intuito discorrer e refletir sobre as mudanças no processo de cuidado e de reabilitação, causadas pela pandemia do COVID-19, em Unidade de Cuidados Continuados Integrados. Com as medidas de distanciamento e isolamento social para a proteção e controle da disseminação do vírus, propostos pela Organização Mundial de Saúde e o Ministério da Saúde, a Unidade suspendeu as visitas dos familiares/cuidadores dos pacientes, em que acarretou no surgimento de alterações emocionais, comportamentais, cognitivas, que influenciam no processo de cuidado e a reabilitação e (não) desejo de permanência dos internos na instituição. Ao longo do tempo, foi necessário repensar sobre o impacto da medida no cuidado e na saúde dos pacientes, na importância do diálogo com os familiares/cuidadores, com a Rede de Atenção à Saúde e, principalmente, com a Atenção Primária à Saúde.
Rede na Rua: Trabalho Intersetorial com População em Situação de Rua na Covid-19
Authors: Fernandes Chaves Lira Gabriela , Rodrigues Viana de Sousa Arthur , Inocencio Barbosa Ellen , Maria Souza Santos Luana 
Published: August 2022

O presente artigo tem como objetivo apresentar a construção de estratégias no território da cidade de Taguatinga-DF que visem à perspectiva de saúde mental dos usuários e profissionais que atendem população em situação de rua na pandemia da COVID–19. Este artigo consolida-se como relato de experiência de um projeto desenvolvido por residentes de um programa de residência multiprofissional em saúde mental do adulto e utilizou a metodologia de natureza qualitativa. Para tanto, pauta-se no método marxista (materialismo - histórico - dialético). O projeto foi dividido em quatro fases: 1. Levantamento de instituições que executam serviços direcionados à população em situação de rua da região de Taguatinga–DF; 2. Contato com as instituições; 3. Visita às unidades e definições de estratégias; 4. Execução das atividades. Os resultados demonstram que ações intersetoriais são fundamentais para a construção de rupturas com o modelo hegemônico. Fortalecer e criar ações no território é endossar o cuidado em liberdade, pensar o sujeito em sua integralidade e viabilizar os princípios do SUS, sobretudo nesse contexto de pandemia.
Metallothionein (MT) Expression and SEM-EDX Mapping on <I>Cymodocea serrulata</I> Seagrass
Authors: Dwi Rosalina  , Sofarini Dini , Muhsoni Firman Farid , Munir Misbakhul , Khasanah Ruly Isfatul 
Published: August 2022

Highlight Research
1. Metallothionein is a protein found in seagrass and functions to bind to heavy metals so as not to damage the seagrass body tissue

Abstract
Lead is a very dangerous heavy metal for organisms because it is carcinogenic, can cause mutations, takes a long time to decompose and has unchanging toxicity. This study on the seagrass Cymodocea serrulata aimed to determine the metallothionein expression quantitatively using the Elisa (Enzyme-Linked Immunosorbent Assay) method and describe the surface structure of C. serrulata roots, rhizomes, and leaves that were exposed to lead using SEM and EDX Mapping methods. The results showed that metallothionein was abundant in C. serrulata tissues (leaves>roots>rhizomes) in both South Bangka and Ketawai Island. SEM showed changes in the morphology and cell size of C. serrulata exposed to lead. EDX and Mapping showed the substance elements found in seagrass tissue. Pb was detected in C. serrulata roots (0.22%) and leaves (0.6%) in South Bangka. This indicates that the seagrass plants are able to absorb heavy metals into their body tissues.
Concentrations of Heavy Metals in Three Brown Seaweed (Phaeophyta: Phaeophyceae) Collected from Tourism Area in Sanur Beach, Coast of Denpasar, Bali and Public Health Risk Assessment
Authors: Rosiana I Wayan , Wiradana Putu Angga , Permatasari Anak Agung Ayu Putri , Pelupessy Yesha Ainensis El G. , Dame Matius Victorino Ola , Soegianto Agoes , Yulianto Bambang , Widhiantara I Gede 
Published: August 2022

Highlight Research

Brown seaweed heavy metals content varies between species
Risk assessment showed low health risk for heavy metal from intake of the three brown seaweed
The three types of brown seaweed did not show carcinogenic properties to metal Arsenic (As)

Abstract
Marine brown seaweed are known as one of the potential biological agents to be developed as functional food and medicinal sectors. This study aims to examine the concentration of heavy metals (Pb, Cd, Hg, and As) in brown algae (Sargassum aquifolium, Padina australis, and Turbinaria ornata.) and the possible exposure to health risks caused by consumption. Heavy metal concentrations were determined using Atomic Absorption Spectroscopy (AAS) on brown seaweed samples obtained from three different sites. The average concentration of heavy metals in the dry weight of brown seaweed remains within the guidelines established by The Food and Drug Supervisory Agency (BPOM) Number 32 of 2019 concerning the Safety and Quality of Traditional Medicines, which is then used to calculate the estimated daily intake (EDI), target hazard quotient (THQ and TTHQ), and target cancer risk (TCR) for arsenic associated with food exposure to potentially toxic metallic elements. Each species of brown seaweed has a THQ and TTHQ level of <1, indicating that one or more toxic metal elements in the same meal provide no significant non-carcinogenic risk. The TCR for arsenic in these seaweeds are all less than 1 x 10-4, indicating no cancer risk. There are no chronic health hazards related with the ingestion of brown seaweed harvested from the coast of Sanur Beach at Denpasar, Bali.
DNA Barcoding on Indian Ocean Squid, <I>Uroteuthis duvaucelii</I> (D’Orbigny, 1835) (Cephalopoda: Loliginidae) from the Java Sea, Indonesia
Authors: Norma Afiati  , Subagiyo Subagiyo , Handayani Christina Retna , Hartati Retno , Kholilah Nenik 
Published: August 2022

Highlight ResearchThe possibility of U. duvauceli be a polymorph cryptic species is further reinforced by the results of the present study.Phylogenetic analysis showed two separate clusters though did not perfectly segregate north to the south waters.Individuals in cluster II from the south coast showed the highest polymorphism levels by 40 segregating sites (S), compared to only 2-4 sites in that of cluster IFrequencies of A, C, G, and T in mitochondrial DNA of all specimens skewed toward A and T, with T being the most preferred and G being the least preferred nucleotideAll specimens showed no amino acid frequency for glutamic acid. Further, all four individuals in cluster II (south coast) were also have no amino acid frequencies for aspartic acid and valine as well.AbstractUroteuthis duvauceli (D’ Orbigny, 1835) also known as the Indian Ocean Squid is a highly important commercial marine organism along the Java coast, Indonesia. Based on genetic variation this species-complex are polymorphic and cryptic. In the present study, the genetic diversity and stock structure of loliginid squid U. duvauceli is investigated using a mitochondrial gene cytochrome oxidase subunit I (COI). Samples were collected by hand-jigging onboard of an 8hp small fisher-boat equipped with a few lamps during May to August 2015, May 2016 and August to November 2018. Sample collection started at dusk until midnight. The attractor was a weighed-quill attached to nylon string, manually immersed into the water and pulled quickly and continuously for about 3-5 minutes at each effort. The determination was conducted with BLAST. Phylogenetic analysis showed two separate clusters with 100% bootstrap value, in which cluster II from Palabuhanratu has divergences of 5.9 - 7.0%, compared to cluster I. Genetic variations exist within and among individuals over the locations. Palabuhanratu individuals have the highest polymorphism levels compared to other locations as shown by 40 segregating sites (S). Frequencies of A, C, G, and T in mtDNA of the 20 specimens U. duvauceli are biased toward A and T, with T being the most favoured and G being the least favoured nucleotide. All specimen showed no amino acid frequency for glutamic acid and all four individuals in cluster II (south coast) also have no amino acid frequencies for aspartic acid and valine as well.
Safety, Adherence, Enzymatic Activities, and Application Effects of Oral Probiotic Candidates for Shortfin Eel (Anguilla bicolor bicolor)
Authors: Dewanti Andita Ratih , Putri Anggi Octari , Istiqomah Indah Istiqomah , Isnansetyo Alim 
Published: August 2022

Highlight Research

The shortfin eel elver bicolor bicolor was tested for the safety of Enterobacter sp. JC05, Lactococcus sp. JAL37, and Bacillus sp. PCP1
The ability of bacterial strains to adhere to epithelial cells of shortfin eel epithelial cells was demonstrated
The bacterial strains' proteolytic, cellulolytic, and lipolytic activities were detected
Oral administration of the bacterial cocktail lowered overall viable bacterial count but did not affect the shortfin eel's intestinal histological characteristics

Abstract
Aquaculture of the shortfin eel (Anguilla bicolor bicolor) has been plagued by low survival and growth due to the low tolerance to water quality and feed. The microbiota and shape of the fish intestinal tract influence the immune and digestive systems. The use of bacterial probiotics is fascinating to enhance the digestion system. This study aimed to characterize bacterial probiotic candidates' safety and potential probiotic features for shortfin eel (A. bicolor bicolor) aquaculture. The safety, adherence, and enzymatic activity of three bacterial strains (Bacillus sp. PCP1, Lactococcus sp. JAL 37, and Enterobacter sp. JC05) were investigated. An oral application test was performed on shortfin eel (n=880, 15 g) every four days with 0, 3x103, 3x105, and 3x107 CFU/g diet dosages in quadruplicates for two months. At the end of the experiment, total cultivable bacteria and intestinal morphology were assessed. Based on the hemolytic test and intraperitoneal injection, the bacterial strains were considered harmless. In an in vitro investigation, the bacteria attached to shortfin eel intestinal epithelial cells. An agar diffusion method validated the proteolytic, lipolytic, and cellulolytic activities. In vivo feeding tests with the bacterial cocktail lowered the total viable bacteria in the gut while preserving the gut histology. Bacterial strains of the present study are potential probiotic candidates for shortfin (A. bicolor bicolor) aquaculture.
Preiser's Disease as a Consequence of Scaphoid Hypoplasia After Remote Index Finger Repositioning
Authors: Pipkin William , Frangenberg Alex , Pientka William F. 
Published: August 2022

Case:
We present the case of a patient who developed scaphoid avascular necrosis in the setting of scaphoid hypoplasia, 37 years after an index finger repositioning for thumb agenesis, due to Holt-Oram syndrome.

Conclusion:
To the best of our knowledge, this is a unique case of scaphoid avascular necrosis in a patient with Holt-Oram syndrome with scaphoid hypoplasia who underwent index finger repositioning 37 years earlier. Her wrist pain was successfully treated with scaphoid excision and intercarpal fusion.
Low-stress livestock handling protects cattle in a five-predator habitat
Authors: Louchouarn Naomi X , Treves Adrian 
Published: August 2022

Abstract
Given the ecological importance of top predators, societies are turning to non-lethal methods for coexistence. Coexistence is challenging when livestock graze within wild predator habitats. We report a randomized, controlled experiment to evaluate low-stress livestock handling (L-SLH), a form of range riding, to deter grizzly (brown) bears, gray wolves, cougars, black bears, and coyotes in Southwestern Alberta. The treatment condition was supervision by two newly hired and trained range riders and an experienced L-SLH-practicing range rider. This treatment was compared against a baseline pseudo-control condition of the experienced range rider working alone. Cattle experienced zero injuries or deaths in either condition. We infer that inexperienced range riders trained and supervised by an experienced rider did not raise or lower the risk to cattle. Also, predators did not shift to the cattle herds protected by fewer range riders. We found a correlation suggesting grizzly bears avoided herds visited more frequently by range riders practicing L-SLH. More research is required to compare different forms of range riding. However, pending experimental evaluation of other designs, we recommend use of L-SLH. We discuss cobenefits of this husbandry method.
Fibrillin microfibril structure identifies long-range effects of inherited pathogenic mutations affecting a key regulatory latent TGFβ-binding site
Authors: Baldock Clair , Godwin Alan , Thomson Jennifer , Holmes David , Adamo Christin , Sengle Gerhard , Sherratt Michael , Roseman Alan , Dajani Rana , Zhang Xinyang 
Published: August 2022

Abstract
Genetic mutations in fibrillin microfibrils cause serious inherited diseases such as Marfan syndrome (MFS) and Weill-Marchesani syndrome (WMS). These diseases typically show major dysregulation of tissue development and growth, particularly in skeletal long bones, but links between the mutations and the diseases are unknown. In this study, we reveal the detailed cryo-EM structure of native fibrillin microfibrils from mammalian tissue. The major bead region showed pseudo 8-fold symmetry where the N-and C-termini reside. Based on this structure, we show a WMS-deletion mutant induces a rearrangement blocking interaction with latent TGFβ-binding protein (LTBP)-1 at a remote site. Separate deletion of this binding site resulted in the assembly of shorter fibrillin microfibrils with structural alterations. The integrin αVβ3-binding site was also mapped onto the microfibril structure. These results establish that in complex extracellular assemblies, such as in fibrillin, mutations may have long-range structural consequences to disrupt growth factor signalling and cause disease.
Clinical characteristic and survival analysis of distant metastatic sarcomatoid renal cell carcinoma: A study of 378 patients
Authors: xing zibao , Chen Yongjie , Cai Gaoping , Chen Shiwei 
Published: August 2022

Abstract
Objective:To analyse the clinical characteristic and survival of distant metastatic sarcomatoid renal cell carcinoma(sRCC), compare the differences between single lung metastases and single bone metastases, and identify the prognostic factors for distant metastatic sRCC patients.Methods: Data from the SEER database between 2004 and 2016 were analyzed, overall survival (OS) between different groups were compared by the Kaplan-Meier method and the Log-rank test, prognostic factors associated with OS were identified by univariate and multivariate Cox proportional hazards models.Results: Among the 378 patients with distant metastatic sRCC, 128 were single lung metastases(33.86%), 66 were single bone metastases(17.47%), 59 were “lung + bone” metastases(15.60%), and the total number of other sites of metastases was 125(33.07%).Patients with single lung metastases had a higher proportion of T3 and T4 stages than those with single bone metastases( P＜0.001).The median OS of 378 patients was 4 months, and there was no statistically significant difference in OS between patients with single lung metastases and single bone metastases (χ2=4.643, P = 0.200). Surgery(cytoreductive nephrectomy) and chemotherapy were good prognostic factors for patients with distant metastatic sRCC regardless of sites of metastases , as well as for patients with single lung metastases,while chemotherapy was not found to affect the OS of patients with single bone metastases.Conclusion: Distant metastatic sRCC had a very poor prognosis, single lung metastases and single bone metastases were main metastatic sites and had different characteristics and prognostic factors,patients should receive specific treatment and management.
The economical and reliable image free navigation system for acetabular cup implantation in total hip arthroplasty
Authors: PATRO BISHNU PRASAD , Mahapatra Manas Mohan , Jain Divesh 
Published: August 2022

Abstract
Introduction: Implantation of acetabular cup is the cornerstone in total hip arthroplasty (THA). Functional outcome of THA is related to acetabular inclination and anteversion besides other parameter like; stability, limb length alignment, capsule closure etc. Various navigation and robotic equipment’s are available to assist the surgeon to place the hip implant in right version. High cost, steep learning curve and safety of equipment has restricted its use less than one percent.Materials and Methods:We have designed an image free navigation device to assist the surgeon to implant the acetabular cup in right inclination and anteversion. The experiment has been performed on an artificial hip bone structure. Historically a total of 50 iteration of the angle measurements were performed to access accuracy of the device. The images from each of the measurement was imported in ImageJ software to find out the actual angle. The principle of openCV is used for image processing in our navigation device.Results:The results of the study found the angle of inclination mean error of 0.15° with standard deviation of 0.44° with a p value <0.05, which is very significant. Similarly the angle of anteversion mean error was -1.06° with standard deviation of 0.34° and p value < 0.05, which is very significant. The tool provides a good accuracy with an error of less than1.5-2 degrees.Conclusion:Our image processing device delivered promising results with regards to acetabular inclination and acetabular anteversion in invitro studies. The tool is compact, can be easily transported in a briefcase and can be used without any extensive training. Besides an initial cost of few thousand rupees, the recurring cost in each surgery is less than 200 rupees. We presume this is the world’s most economical navigation tool which is reproducible and reliable.