bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison between Oblique Lateral Interbody Fusion and Minimally Invasive Transforaminal Lumbar Interbody Fusion in Degenerative Lumbar Stenosis: An Age-, Sex-, and segment-matched Cohort Study
Authors: Wang Dechun , Liu Lantao , Xue Hui , Han Zhiyuan , Jiang Lianghai , Chen Longwei 
Published: March 2022

Abstract
Objectives: To compare outcomes after oblique lateral interbody fusion (OLIF) versus minimally invasive transforaminal lumbar interbody fusion (MISTLIF) with bilateral decompression via unilateral approach for treating mild to moderate symptomatic degenerative lumbar spinal stenosis (DLSS).Methods: We retrospectively compared patients who underwent single-level (L4/5) OLIF with an age-, sex-, and segment-matched MISTLIF with bilateral decompression via unilateral approach cohort. Perioperative data were collected for the operative time, intraoperative blood loss, drainage in the first postoperative day, postoperative hospital stay, cost, intraoperative fluoroscopy, and complications. Lumbar radiographs were measured for changes in posterior intervertebral space height (PISH), intervertebral space foramen height (IFH), intervertebral foramen area (IFA), and area of ​​the spinal canal (ASC). Clinical and psychological outcomes included the visual analog scale (VAS), Oswestry Disability Index (ODI), and hospital anxiety and depression scale (HADS).Results: 35 OLIF patients were compared with 35 MISTLIF patients in L4/5 DLSS. The OLIF group had shorter bedtime, postoperative hospital stays, less intraoperative and postoperative blood loss (all P<0.05), but had more times of intraoperative fluoroscopy, longer operative time, and higher cost (all P<0.05). The complication rates were equivalent (OLIF vs MISTLIF: 22.86% vs 17.14%). PISH (11.94±1.78mm vs 9.42±1.94mm, P<0.05), IFH (23.87±3.05mm vs 21.41±2.95mm, P<0.05), and IFA (212.14±51.82mm2 vs 177.07±51.73mm2, P<0.05) after surgery were significantly increased in the OLIF group. The ASC was increased significantly after the operation in both groups, but the ASC in the MISTLIF group was increased significantly more than that in the OLIF group (450.04±66.66mm2 vs 171.41±58.55mm2, P<0.05). The lumbar VAS scores at 1 month (1.89±0.87 vs 2.34±0.84, P=0.028) and 6 months (1.23±0.97 vs 1.80±0.99, P=0.018) after operation in the OLIF group were significantly lower. There were no significant differences in lower extremity VAS and ODI scores between the two groups. Compared with MISTLIF group, HADS scores on postoperative day 3 (2.91±1.46 vs 4.89±1.78, P<0.05) and prior to hospital discharge (PTD) (2.54±1.38 vs 3.80±1.78, P=0.002) in the OLIF group were decreased significantly. Conclusions: OLIF showed more advantages of less surgical invasion, lower incidence of postoperative low back pain, faster postoperative recovery, and less anxiety compared with MISTLIF. Regardless of cost, OLIF seems to be a better option to treat mild to moderate symptomatic DLSS.
Fusion transcript analysis reveals slower response kinetics than multiparameter flow cytometry in childhood acute myeloid leukemia
Authors: Fogelstrand Linda , Karlsson Lene , Nyvold Charlotte Guldborg , Soboli Anastasia , Johansson Pegah , Palmqvist Lars , Tierens Anne , Hasle Henrik , Lausen Birgitte , Jónsson Ólafur Gisli , Jürgensen Gitte Wulff , Ebbesen Lene Hyldahl , Abrahamsson Jonas 
Published: March 2022

Abstract
Background: Analysis of measurable residual disease (MRD) is increasingly being implemented in the clinical care of children and adults with acute myeloid leukemia (AML). However, MRD methodologies differ and discordances in results lead to difficulties in interpretation and clinical decision-making. The aim of this study was to compare results from reverse transcription quantitative polymerase chain reaction (RT-qPCR) and multiparameter flow cytometry (MFC) in childhood AML and describe the kinetics of residual leukemic burden during induction treatment. Methods: In 15 children who were treated in the NOPHO-AML 2004 trial and had fusion transcripts quantified by RT-qPCR, we compared MFC with RT-qPCR for analysis of MRD during (day 15) and after induction therapy. Eight children had RUNX1::RUNX1T1, one CBFB::MYH11 and six KMT2A::LLT3. Results: When ≥0.1% was used as cut-off for positivity, 10 of 22 samples were discordant. The majority (9/10) were MRD positive with RT-qPCR but MRD negative with MFC, and several such cases showed presence of mature myeloid cells. Fusion transcript expression was verified in mature cells as well as in CD34 expressing cells sorted from diagnostic samples. Conclusions: Measurement with RT-qPCR suggests slower response kinetics than indicated from MFC, presumably due to the presence of mature cells expressing fusion transcript. The prognostic impact of early measurements with RT-qPCR remains to be determined.
A broad-spectrum antiviral fusion protein, Pheromonicin demonstrated protective efficacy against SARS-CoV-2 variants in vitro and in vivo models
Authors: Qiu Xiao-Qing , Lu Shuaiyao , Cao Ke-Fu , Yang Cheng-Yun , Zhou Yanan , Tang Jian-Yong , Zhang Dong , Luo Feng-Yu , Li Hong-Fa , Li Rong-Qi 
Published: March 2022

Abstract
The unprecedented coronavirus disease (COVID-19) epidemic has created a worldwide public
health emergency, and there is an urgent need to develop an effective antiviral drug to control this
severe infectious disease. Here, we found that the E, or M membrane proteins of coronavirus could be targeted by a 28-residue antibody mimetic by fusing two antibody Fab complementarity-determining regions (VHCDR1 and VLCDR3) through a cognate framework region (VHFR2) of the antibodies which recognize the coronavirus E or M proteins. We constructed a fusion protein, pheromonicin-covid-19 (PMC-covid-19), by linking colicin Ia, a bactericidal molecule produced by E.coli which kills target cells by forming a voltage-dependent channel in target lipid bilayers, to that antibody mimetic. The E, or M protein/antibody mimetic interaction initiated the formation of irreversible PMC-covid-19 channel in the covid-19 envelope and infected host cell membrane resulting in leakage of cellular contents. PMC-covid-19 demonstrates broad-spectrum protective efficacy against tested variants of coronavirus severe acute respiratory syndrome (p<0.01-0.0001). PMC-covid-19 significantly altered outcomes of in vivo fatal covid-19 challenge infection without evident toxicity, making it an appropriate candidate for further clinical evaluation.
An epidemic digital management framework for healthcare organizations based on multi-platform
Authors: Mao Zijun , Wu Jingyi , Deng Yunqi , Zhang Zhixin 
Published: March 2022

Abstract
Background: Digital alternatives offer the potential for agility and resilience in epidemic management. The fragmentation of digital technology applications urgently requires a holistic digital epidemic management framework.Objectives: Our research aims to design a digital framework for epidemic management that can apply digital technologies to assist healthcare providers with governance of epidemiological information.Methods: The design science research methodology was applied to create an artifact of a digital healthcare management framework. A systematic literature review was conducted to sort out the issues and motivations, a case study was applied to demonstrate the artifact, and users evaluated its effectiveness by scoring it.Results: The data platform ecosystems-based framework is constructed from three platforms. The data platform integrates and mines multiple data resources. The technology platform establishes a domain-wide shared technology model. The service platform enables service requests for stakeholders in the epidemic.Conclusions: The digital epidemic management framework allows micro-servicing and visualization of all capabilities of healthcare organizations. It allows for the rapid establishment and reuse of a healthcare organization’s overall capabilities in public health emergencies, while sustainably focusing on the requirements of patients and managers. This new digital solution can improve digital epidemic prevention and control management.
Research on the Impact of Internet Use on the Health Status of the Working Age Population in Digital Economy --An Empirical Analysis Based on 2018 CFPS Data
Authors: Zhang Haoyuan , Zhou Qian 
Published: March 2022

Abstract
Background
China's economic structure is changing from labor-intensive to technology-intensive due to the emergence of low fertility and the increasingly serious aging problem, so it is of great importance to improve the health status of the working age population. Meanwhile, with the accelerated integration of digital technology and real economy, the Internet has increasingly become an important driving force for economic growth and social development in China and has also played a big role in health care, especially under the COVID-19. Therefore, it is worth further exploring how Internet use affects the health of the working age population.
Method
We used data from China Family Panel Studies (CFPS) in 2018 and applied the Ordered-Probit model to study the relationship between Internet use and the health status of working age population
Results
The Internet use was significantly and positively related to the health status of the working age population; there was an inverted U-shaped relationship between the intensity of Internet use and individual health; the Internet use affected individual health through information acquisition. The heterogeneity analysis showed that the health promotion of Internet use was stronger for people aged from 32 to 50 years old/ in rural areas/ at low education level/ in the eastern region. Further analysis showed that the Internet use can reduce health inequalities caused by different levels of socioeconomic status.
Conclusions
Internet, as an information media, has a significantly positive impact on individual health, but excessive use is not conducive to the improvement of health status. However, due to the large base of netizens in China and the unbalanced development among regions, Internet use can have a differential impact on the health status of different groups. In addition, the Internet enhances the accessibility of health care knowledge and compensates for the lack of access to information for low socioeconomic groups, thus improving their health status and reduce health inequalities among different classes.
Vacuum Evaporation of High Quality Cspbbr3 Thin Films for Efficient Light-Emitting Diodes
Authors: Bai Tianxinyu , Yi Lixin 
Published: March 2022

Abstract
The all-inorganic lead halide perovskite has become a very promising optoelectronic material due to its excellent optical and electrical properties. Device performances are currently hindered by crystallinity of the film and environmental stability. Here, We adopted dual-source co-evaporation method to prepare CsPbBr3 films. By adjusting and controlling the co-evaporation ratio and substrate temperature, we obtained stable and large grains with uniform morphology CsPbBr3 films. Films with smooth surface and large grains exhibit efficient photon capture, fast carrier transport, and suppressed ion migration. Therefore, in this paper, by refining the annealing conditions, the effects of annealing temperature and time on the films were studied in detail. The CsPbBr3 films were annealed under suitable annealing temperature and time in ambient air, films with high quality and crystallinity, average grains size up to ~ 2.5 µm, films could maintain stability in ambient air for 130 days. The corresponding LED shows the full width at half-maximum (FWHM) of the green EL spectrum is as narrow as 18 nm, the devices have a low turn-on voltage VT ~ 3 V and can work continuously for 12 hours in ambient air.
Ginsenoside Rg5 Boost Enhances the Therapeutic Efficacy of Immune Checkpoint Blockade through NF- κB Signaling in Colorectal Cancer
Authors: Zhang Jiwei , Feng Yutao , Ma Fen , Mo Shaowen , Huang Yiming , Sun Xun , Song Mingxu , Yang Fan , Wu Enjiang , Sun Yidi , Wang Zhengtao , Li Yang 
Published: March 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Millennial-scale solar variability in tree rings of northern Fennoscandia at the end of the Holocene
Authors: Kasatkina Elena A. , Shumilov Oleg I. , Timonen Mauri , Potorochin Evgeniy O. 
Published: March 2022

Abstract
There is growing evidence that the Sun is one of the main drivers of the Earth’s climate in the past. To investigate the possible Sun-climate connection during the Holocene, the super-long tree-ring chronology covering the period from 5634 BC and 2004 AD is analyzed. The chronology used, consists of more than 1200 series including ones from sedimentary archives of small lakes in the region of northern Fennoscandia (68º- 70ºN, 20º - 30ºE). All series are dendrochronologically dated to calendar years. As indicator of solar activity we use a reconstruction of total solar irradiance (TSI) covering 9300 years which is based on a composite using the cosmogenic radionuclide Be^10 measured in ice cores GRIP (Greenland) and South Pole (Antarctic), and also on neutron monitor data (Steinhilber et al. 2009). To examine the relationship between tree’s growth and solar activity, the MTM, wavelet decomposition and wavelet coherence analysis were applied to the time series. MTM spectral analysis identifies in tree-ring series the main solar cycles at ~ 200 yr (de Vries or Suess) ~350 yr (unnamed) and ~ 900 yr (Eddy). The strongest cross-wavelet correlation was discovered between the millennium-cycle components of TSI and tree-ring width variations. This Eddy cycle, which was recently discovered in solar activity, remains both strong and stable through almost the entire Holocene, and it reappears again at lower ( ~ 1300 years) frequencies since around AD 200 till now. The millennial-cycle components are modulated by a ~ 6500-year period of solar or geomagnetic origin. Our results raise questions on the subject regarding the end of the Holocene and transition to the next glacial period. In any case, the results presented confirm the complex and nonlinear nature of the Sun-climate relationship during the Holocene epoch.
First Nationwide Survey of Infection Prevention and Control among Healthcare Facilities in Japan: Impact of the National Regulatory System
Authors: Nomoto Hidetoshi , Saito Hiroki , Ishikane Masahiro , Gu Yoshiki , Ohmagari Norio , Pittet Didier , Kunishima Hiroyuki , Allegranzi Benedetta , Yoshida Masaki 
Published: March 2022

Abstract
Background: Infection prevention and control (IPC) measures in Japan are facilitated by a financial incentive process at the national level, where facilities are categorized into three groups (Tier 1, Tier 2, or no financial incentive). However, its impact on IPC at the facility level using a validated tool has not been measured.Methods: A nationwide cross-sectional study was conducted from August 2019 to January 2020 to evaluate the situation of IPC programs in Japan, using the global IPC Assessment Framework (IPCAF) developed by the World Health Organization. Combined with the information on the national financial incentive system, the demographics of facilities and each IPCAF item were descriptively analyzed. IPCAF scores were analyzed according to the facility level of care and the national financial incentive system for IPC facility status, using Dunn-Bonferroni and Mann-Whitney U tests. Results: Fifty-nine facilities in Japan responded to the IPCAF survey: 34 private facilities (57.6%) and 25 public facilities (42.4%). Of these, 11 (18.6%), 29 (49.2%), and 19 (32.3%) were primary, secondary, and tertiary care facilities, respectively. According to the national financial incentive system for IPC, 45 (76.3%), 11 (18.6%), and three (5.1%) facilities were categorized as Tier 1, Tier 2, and no financial incentive system, respectively. Based on the IPCAF total score, more than half of the facilities were categorized as “Advanced” (n = 31, 55.3%), followed by “Intermediate” (n = 21, 37.5%). The IPCAF total score increased as the facility level of care increased, while no statistically significant difference was identified between the secondary and tertiary care facilities (p= 0.79). There was a significant difference between Tier 1 and Tier 2 for all core components and total scores. Core components 5 (multimodal strategies for implementation of IPC interventions) and 6 (monitoring/audit of IPC and feedback) were characteristically low in Japan with a median score of 65.0 (interquartile range 40.0–85.0) and 67.5 (interquartile range 52.5–87.5), respectively. Conclusions: The national financial incentive system was associated with IPC programs at facility level in Japan. The current financial incentive system does not emphasize the multimodal strategy or cover monitoring/audit, and an additional systematic approach may be required to further promote IPC for more practical healthcare-associated infection prevention.
Assessment of selected interleukins (IL-6, IL-17A, IL-18, IL-23) and chemokines (RANTES, IP-10) in children with acute and chronic urticaria
Authors: Góra Anna , Przybył Maciej , Świętochowska Elżbieta , Machura Edyta 
Published: March 2022

Abstract
Background Urticarial lesions develop as a result of the activation of mast cells which, through the release of mediators, influence the formation of local inflammatory infiltrates. Changes in the expression of many cytokines and chemokines are observed in the course of urticaria.The aim of the study was to evaluate serum levels of interleukin (IL)-6, IL-17A, IL-18, IL-23, regulated on activation, normal T cell expressed and secreted (RANTES) and interferon (IFN)-γ-inducible protein-10 (IP-10) in children with acute and chronic urticaria in comparison to healthy volunteers. Moreover, we made an attempt to identify factors predicting the occurrence and course of urticaria among the studied parameters.MethodsWe retrospectively analyzed 32 children with acute urticaria and 32 children with chronic urticaria. The control group consisted of 40 healthy children. Each patient was clinically evaluated. Serum concentrations of selected cytokines and chemokines were determined by using enzyme-linked immunosorbent assay.ResultsPatients with acute and chronic urticaria had higher concentrations of IL-6 and IL-17A (p<0.001) and lower concentrations of IL-18, IL-23, RANTES and IP-10 (p<0.001) as compared to the control group. IL-6 and IP-10 showed high predictive values for the development of urticaria. There was no correlation of the studied cytokines and chemokines with disease activity.ConclusionsIn children with acute and chronic urticaria, the cytokine profile differs compared to healthy subjects. IL-6 and IP-10 are useful in differentiating children with urticaria and healthy ones. The search for factors predicting the course of the disease requires further studies.
Paper-based Broadband Flexible Photodetectors with van der Waals materials
Authors: Mahmoodi Erfan , Amiri Morteza Hassanpour , Salimi Abdollah , Frisenda Riccardo , Flores Eduardo , Ares José R. , Ferrer Isabel J. , Castellanos-Gomez Andres , Ghasemi Foad 
Published: March 2022

Abstract
Layered metal chalcogenide materials are exceptionally appealing in optoelectronic devices thanks to their extraordinary optical properties. Recently, their application as flexible and wearable photodetectors (PDs) have received a lot of attention. Herein, broadband and high-performance paper-based PDs were established in a very facile and inexpensive method by rubbing molybdenum disulfide (MoS2) and titanium trisulfide (TiS3) crystals on papers. Transferred layers were characterized by SEM, EDX mapping, and Raman analyses, and their optoelectronic properties were evaluated in a wavelength range of 405-810 nm. Although the highest and lowest photoresponsivities were respectively measured for TiS3 (1.50 mA/W) and MoS2 (1.13 μA/W) PDs, the TiS3-MoS2 heterostructure not only had a significant photoresponsivity but also showed the highest on/off ratio (1.82) and fast response time (0.96 s) compared with two other PDs. This advantage is due to the band offset formation at the heterojunction, which efficiently separates the photogenerated electron-hole pairs within the heterostructure. Numerical simulation of the introduced PDs also confirmed the superiority of TiS3-MoS2 heterostructure over the other two PDs and exhibited a good agreement with the experimental results. Finally, MoS2 PD demonstrated very high flexibility under applied strain, but TiS3 based PDs suffered from its fragility and experience a remarkable drain current reduction at strain larger than ± 0.33%. However, at lower strains, all PDs displayed acceptable performances.
Quantitative proteomics identified circulating biomarkers in lung adenocarcinoma diagnosis
Authors: Chen Hongyu , Lai Xiaoqin , Zhu Yihan , Huang Hong , Zeng Lingyan , Zhang Li 
Published: March 2022

Abstract
Background: Lung cancer is a malignant tumor with a high incidence and poor prognosis. Lung adenocarcinoma is the most common type of non-small cell lung cancer, accounting for approximately 40% of lung cancers. Methods: This study was based on total proteomic analysis and parallel reaction monitoring validation of peripheral blood from 20 lung adenocarcinoma patients and 20 healthy individuals. Furthermore, differentially expressed proteins closely related to prognosis were analysed using Kaplan–Meier Plotter and receiver operating characteristic curve (ROC) curve analysis. Results: The candidate proteins GAPDH and RAC1 showed the highest connectivity with other differentially expressed proteins between the lung adenocarcinoma group and the healthy group using STRING. Kaplan–Meier Plotter analysis showed that lung adenocarcinoma patients with positive ATCR2, FHL1, RAB27B, and RAP1B expression had observably longer overall survival than patients with negative expression (P<0.05). The high expression of ARPC2, PFKP, PNP, RAC1 was observably negatively correlated with prognosis (P<0.05). 17 out of 27 proteins showed a high area under the curve (>0.80) between the lung adenocarcinoma and healthy plasma groups. Among those proteins, UQCRC1 had an area under the curve of 0.960, and 5 proteins had an area under the curve from 0.90 to 0.95, suggesting that these hub proteins might have discriminatory potential in lung adenocarcinoma, P<0.05. Conclusions: These findings provide UQCRC1, GAPDH, RAC1, PFKP have potential as novel biomarkers for the early screening of lung adenocarcinoma.