bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterogeneous Photocatalytic degradation of Nevirapine in wastewater using the UV/TiO2/H2O2 process
Authors: Ncube Pauline , Zvinowanda Caliphs , Belaid Mohamed , Ntuli Freeman 
Published: July 2022

Abstract
This work sought to study the elimination of NVP from simulated wastewater using the UV/TiO2/H2O2 system. The main objectives were to evaluate the influence of operating parameters, reaction kinetics, contributing reactive species, degradation pathway, and catalyst reusability. The research surface methodology (RSM) was utilized for optimizing process variables and for statistical analysis. Five process variables (pH, NVP concentration, TiO2 dosage, H2O2 concentration, and irradiation time) were optimized with the responses set as NVP and Total organic carbon (TOC) removals (%), respectively. 89.23% (NVP) and 85.71% (TOC) removals were achieved under optimum conditions. All the experimental factors were significant for NVP removal. pH was the most dominant factor, with the highest removals obtained under acidic conditions (pH 3). NVP removal conformed to the pseudo-first-order model with a rate constant (k1) of 0.03676 min-1. Increasing pH reduced the rate constant by 75.38%, while there was an increase of 43.55% with H2O2. NVP degradation was largely driven by the •OH and h+VB. The photocatalyst demonstrated good stability against NVP loss over four cycles. Although the UV/TiO2/H2O2 process has shown promising results in the removal of pharmaceuticals and dyes in wastewater, its application for the degradation of ARVs remains limited. As far as the authors know, the UV/TiO2/H2O2 process and RSM have not yet been reported for the degradation and optimization of NVP in wastewater, respectively. The findings of this work illustrate that the UV/TiO2/H2O2 system, applied with RSM can effectively degrade and mineralize NVP in wastewater.
RCAN1 deficiency aggravates sepsis-induced cardiac remodeling and dysfunction through accelerating mitochondrial pathological fission
Authors: Zhuang Jinqiang , Chen Liming , Li Gongke , Xia Le , Wu Shaohong , Leng Junling , Tao Xuefei , Hong Jiang , Wu Yong , Wang Shijun , Yuan Ruijun 
Published: July 2022

Abstract
Background Cardiac dysfunction and remodeling are serious complications of sepsis and are correlated with high mortality. RCAN1 is a feedback regulator of cardiac hypertrophy. Here we aim to investigate role of RCAN1 in septic cardiomyopathy. Methods RCAN1-/- mice and WT mice were randomly divided into control and LPS-induced groups, some with Midiv-1 or KN93 treatment. The protein levels of RCAN1, p-ERK1/2, NFAT3, Drp1, p-Drp1, p-CaMKII in cardiac tissue or cultured cardiomyocytes were detected by Western blotting. Myocardial function was assessed by echocardiography. Cardiac hypertrophy and fibrosis were detected by H&E and Masson's trichrome staining. Mitochondrial morphology was examined by transmission electron microscope. Serum level of LDH was detected by ELISA. Results Our data showed that RCAN1 was downregulated in septic mouse heart and LPS-induced cardiomyocytes, and RCAN1-/- mice had impaired cardiac function and severe myocardial hypertrophy and fibrosis. NFAT3 and p-ERK1/2 were significantly increased in heart tissue of RCAN1-/- mice. Further, RCAN1 deficiency aggravated sepsis-induced cardiac mitochondrial injury as indicated by increased ROS production, pathological fission and the loss of mitochondrial membrane potential. Inhibition of fission by Mdivi-1 reversed LPS-induced cardiac hypertrophy, fibrosis and dysfunction in RCAN1-/- mice. Moreover, RCAN1 depletion caused mitochondrial translocation of CaMKII, which promoted fission and septic hypertrophy. Inhibition of CaMKII with KN93 reduced excessive fission, improved LPS-induced cardiac remodeling and dysfunction in RCAN1-/- mice. Conclusions Our finding demonstrated that RCAN1 deficiency aggravated mitochondrial injury and septic cardiomyopathy through activating CaMKII. RCAN1 serves as a novel therapeutic target for treatment of sepsis-related cardiac remodeling and dysfunction.
Obestatin Levels in Basal Conditions and During Oral Glucose Tolerance Test in male subjects with Prediabetes and Diabetes
Authors: Bounias Ioannis , Antonoglou Cristos , Papazoglou Dimitrios , Papanas Nikolaos , Tripsianis Gregory , Kotsianidis Ioannis , Stakos Dimitrios A 
Published: July 2022

Abstract
Purpose: The aim of the present study was to evaluate circulating plasma obestatin levels and its’ secretory dynamics in subjects with impaired glucose tolerance (IGT) as well as in patients with type 2 diabetes (T2DM) in basal conditions and in response to oral glucose tolerance test (OGTT). Furthermore we wish to investigate possible associations between obestatin and several metabolic parameters in T2DM. Methods: The study comprised 73 male adults aged 40 - 72 years (31 with T2DM, 17 with IGT and 25 control healthy individuals). Plasma obestatin, insulin and glucose levels were measured at 0, 60, 120 and 180 min following an OGTT. Total area under response curve was determined and calculated for glucose, insulin and obestatin. Results: Significant differences in the kinetics of obestatin levels during OGTT were observed only in the control group (p=0.012), where specifically shown that plasma obestatin concentrations decreased significantly at 120΄ (p=0.005) and 180΄ (p=0.050) compared to 60΄. Plasma obestatin concentrations were significantly lower in the T2DM group compared to controls at baseline (p<0.001) and at 60΄ following OGTT (p=0.007). At 60΄, plasma obestatin concentrations were also significantly lower in the T2DM compared IGT (p=0.044). In addition, both AUC and nadir obestatin levels were lower in T2DM compared to controls (p=0.039 and p=0.001 respectively). In T2DM basal obestatin levels showed a statistically significant positive correlation with triglycerides, fasting insulin, HOMA–IR, HOMA2–IR, HΟΜΑ–β and a negative correlation with Quicki and Matsuda Index. Similarly in the IGT group, basal obestatin levels showed a positive correlation with fasting insulin, HOMA–β and HOMA2–IR. Conclusion: We observed significantly lower plasma obestatin levels in patients with T2DM and those with IGT compared to normoglycemic individuals both in basal conditions and during an OGTT. Moreover, significant associations of obestatin with several metabolic profile indices were also found in T2DM.
Supramolecular Complexes Built of Octahedral [Ta6Cl12(CN)6]3-/4- Clusters and Terpyridine-Metal Complexes
Authors: Zhang JianJun , Zhou H. Andrew , Lachgar Abdessadek 
Published: July 2022

Abstract
The preparation and characterization of two supramolecular complexes from octahedral [Ta6Cl12(CN)6]4-/3- units and 2, 2’: 6’, 2’’-terpyridine (Terpy) metal complexes as building blocks are reported. Single crystal analyses revealed [Mn(Terpy)]2[Ta6Cl12(CN)6]•MeOH (1) features a neutral two-dimensional (2D) framework composed of layers based on [Ta6Cl12(CN)6]4- and [Mn(Terpy)]2+. Each layer is built of 6-connected [Ta6Cl12]2+ and 3-connected Mn(II) nodes bridged by cyanide ligands. The layers are held together by MeOH molecules. The layered structure is maintained after the removal of solvent molecule (MeOH) upon heating, leading to the formation of [Mn(Terpy]2[Ta6Cl12(CN)6] (1’). In the case of using Gd3+, the resultant product was revealed by single-crystal analyses to be an ionic compound [Gd(Terpy)(H2O)4(DMF)2][Ta6Cl12(CN)6]•DMF •3H2O (2). 2 has a three-dimensional (3D) framework built of [Gd(Terpy)(H2O)4(DMF)2]3+ and [Ta6Cl12(CN)6]3- ions connected to each other via extensive hydrogen bonds and p-p interactions between the cations, anions, and solvent molecules. Thermal stabilities of 1‒2 are reported.
Integrative systems vaccinology identification of shared gene signatures between natural dengue infection and vaccination trials
Authors: Plaça Desireé , Fonseca Dennyson , Marques Alexandre , Pour Shahab Zaki , Usuda Júlia , Baiocchi Gabriela , Prado Caroline , Salgado Ranieri , Filgueiras Igor , Freire Paula , Rocha Vanderson , Câmara Niels Olsen Saraiva , Cabrel-Miranda Gustavo , Jurisica Igor , Calich Vera , Giil Lasse , Rivino Laura , Ochs Hans , Schimke Lena , Cabral-Marques Otavio 
Published: July 2022

Abstract
Dengue virus (DENV) infection is a global health problem with no specific therapy or vaccine currently available to combat it. Here we performed a comprehensive analysis of publicly available transcriptome data of patients with natural DENV infection (NDI) and DENV vaccine trials (DVT1, DVT2, and DVT3), identifying common transcriptional signatures according to the time of infection in NDI and DVTs, and disease severity in NDI. We identified 237 common differentially expressed genes (DEGs) between NDI and DVT1. Of those, 20 were commonly affected by dengue vaccination during DVT2 and DVT3. Machine learn analysis ranked the 10 NDI's most critical predictors for disease severity, e.g., IFIT5, ISG15, and HERC5, which play an essential role in the anti-viral immune response. Hence, this work provides new insight into the NDI and vaccine-induced overlapping immune response and suggests novel targets for developing anti-dengue-specific therapies and monitoring the effectiveness of vaccination.
Nutritional status, health risk behaviors, and eating habits are correlated with physical activity and exercise of older hypertensive adults: a cross-sectional study
Authors: Silva Leonardo Santos Lopes Da , Batalhão Daniel De Freitas , Carvalho Anderson Dos Santos , Bohn Lucimere , Ramos Nilo César , Abdalla Pedro Pugliesi 
Published: July 2022

Abstract
Background
Some behaviors can influence compliance to physical activity and exercise during aging. Nutritional status, health risk behaviors, and eating habits may be associated with recommended amounts of physical activity and exercise in healthy older adults. However, these associations are still not clear for older hypertensive adults, that require greater care from health professionals. We aimed to associate the nutritional status, health risk behaviors, and eating habits with recommended amounts of physical activity and exercise practice of older hypertensive adults.
Methods
10,789 older hypertensive adults (70.9 ± 7.4 years) from VIGITEL survey were classified according to physical activity/exercise practice. Binary logistic regression was used to observe the odds ratio (OR) between nutritional status, risk behaviors (screen time, alcohol, and tobacco consumption), and eating habits (minimally/ultra-processed foods consumption score) with recommended amounts of physical activity/exercise.
Results
Highest nutritional status (ORPHYSICAL−ACTIVITY=-2.7%; OREXERCISE=-2.1%), screen time (ORPHYSICAL−ACTIVITY=-10.2%), alcohol consumption (ORPHYSICAL−ACTIVITY=-31.1%; OREXERCISE=-29.4%), tobacco (ORPHYSICAL−ACTIVITY=-38.4%; OREXERCISE=-53.2%) and ultra-processed foods consumption score (ORPHYSICAL−ACTIVITY=-10.1%; OREXERCISE=-11.2%) reduced the odds of being sufficiently active/practicing exercise (p < 0.05). Minimally processed foods consumption score increased the odds of being sufficiently active/practicing exercise (ORPHYSICAL−ACTIVITY=13.2%; OREXERCISE=16.8%, respectively; p < 0.05).
Conclusion
Nutritional status, health risk behaviors, and eating habits influence the odds of older hypertensive adults complying with physical activity and exercise recommendations. The results help outline the adoption of objective strategies aimed at risk factor behaviors in the treatment/control of arterial hypertension in older adults.
Synergistic roles of the phospholipase B homolog Plb1 and the cAMP-dependent protein kinase Pka1 in the hypertonic stress response of Schizosaccharomyces pombe
Authors: Matsuo Yasuhiro , Marcus Stevan , Kawamukai Makoto 
Published: July 2022

Abstract
The phospholipase B homolog Plb1 and the cAMP-dependent protein kinase (PKA) pathway are required by fission yeast, also known as to Schizosaccharomyces pombe, to grow under KCl stress conditions. Here, we report the relative contributions of Plb1 and the cAMP/PKA pathway during the hypertonic stress response. We show that the plb1∆, cyr1∆, and pka1∆ single mutants are sensitive to high concentrations of KCl but insensitive to sorbitol-induced osmotic stress. In contrast, the plb1∆ cyr1∆ and plb1∆ pka1∆ double mutants are hypersensitive to KCl and sorbitol. The cyr1∆ pka1∆ double mutants showed the same phenotype of each single mutant. Growth inhibition due to hypertonic stress in the plb1∆, plb1∆ cyr1∆, and plb1∆ pka1∆ strains was partially rescued by cgs1 deletion—cgs1∆ has constitutively active Pka1—or by the deletion of transcription factor Rst2, which is negatively regulated by Pka1. Pka1-GFP localized in the nucleus and cytoplasm in plb1∆, whereas it localized only in the cytoplasm in cyr1∆, indicating that Plb1 does not regulate Pka1 localization. Glucose limitation downregulates the PKA pathway, and it was accordingly observed that glucose limitation in plb1∆ further increased the strain’s sensitivity to KCl. Growth inhibition by KCl in plb1∆ under glucose-limited conditions was significantly rescued by cgs1∆ and slightly rescued by rst2∆. These findings indicate that, in fission yeast, the glucose-sensing cAMP/PKA pathway is mainly responsible for responding to stressful hypertonic conditions induced by high levels of KCl, and that Plb1 has a subsidiary role in this stress response.
Absence of KLK4-KLKP1 Fusion Corroborate with PTEN Loss in Characterizing Men at Higher Risk for Biochemical Recurrence in Middle Eastern Prostate Cancer Cohort
Authors: Bakker Andrea , Slack Jonathan , Palanisamy Nalla , Carskadon Shannon , Ghosh Sunita , Khalifeh Ibrahim , Bismar Tarek A. 
Published: July 2022

Abstract
Background: KLK4-KLKP1 fusion is a recently described molecular event in prostate cancer (PCa). This new biomarker has not been characterized in the Middle Eastern (ME) population. Objective: We assessed the incidence, characterization, and prognostic value of KLK4-KLKP1 fusion in a ME cohort of men with PCa and assessed the relationship of this marker to commonly known biomarkers (PTEN, ERG, SPINK1). Design, setting and participants: We interrogated a cohort of ME men with localized PCa treated by radical prostatectomy between 2005 and 2015 (n=340). KLK4-KLKP1 fusion status was assessed by RNA Chromogenic in situ hybridization (CISH) and correlated to pathological and clinical parameters.Outcome measurements and statistical analysis: RNA-CISH expression of KLK4-KLKP1 was correlated with prognostic factors, ERG, PTEN and SPINK1 expression and with biochemical recurrence (BCR) post radical prostatectomy.Results and limitations: 51.7% of patients’ samples were positive for KLK4-KLKP1; with the expression more commonly found in cores of PCa (38%) versus non-cancer (20.6%) (p <0.0001) and in lower Gleason Grade Group tumors (1-3) vs (4-5). KLK4-KLKP1 positivity was associated with ERG positivity and inversely associated with PTEN loss. No association was noticed with SPINK1 expression, seminal vesicle invasion, positive surgical margin, pathological stage, or patient age (<50 or ³ 50). When correlating to BCR, only PTEN loss was associated with BCR, and this effect became more pronounced when combined with KLK4-KLKP1 negativity (HR: 2.31, CI: 1.03-5.20, p=0.042). Conclusions: KLK4-KLKP1 expression is more common in ME vs North American (NA) populations. It is associated with ERG positivity and inversely with PTEN loss. KLK4-KLKP1 positivity showed no association with any clinical or pathological parameters, however, it demonstrated a somewhat protective effect when combined with PTEN status. Patient Summary: KLK4-KLKP1 is a newly discovered biomarker expressed in PCa with a higher incidence in the ME population. KLK4-KLKP1 positivity is associated with certain molecular subtypes (ERG-positive, PTEN intact).
SSAR-GNN: Self-Supervised Artist Recommendation with Graph Neural Networks
Authors: Wang Menghan , Rao Xuan , Chen Lisi , Shang Shuo , Zhang Binbin 
Published: July 2022

Abstract
Artist recommendation plays a vital role in the artist domain. Accurate recommendation can help avoid ineffective searches and acquire comprehensive knowledge regarding relationships among artists. However, existing studies mainly focus on artists themselves or artistic works. They are incapable of exploring the relationships among artists in an effective way. In this paper, we study the problem of artist recommendation for the first time. We propose a artist dataset to analyze the similarity relationship from spatial and temporal aspects between artists. Specifically, based on the dataset, we propose a self-supervised learning approach to construct the artist graph. To incorporate the learned graph into existing models, we propose a novel network, SSAR-GNN for recommendation. SSAR-GNN applies a simplified Graph Convolution Network (GCN) on the artist graph to enrich the representation of each artist. Experimental results on the dataset show the effectiveness of our proposed method SSAR-GNN in terms of accuracy.
Identifying Challenges to Critical Incident Decision-Making through a Macro-, Meso-, Micro- Lens: A Systematic Synthesis and Holistic Narrative Analysis
Authors: May Brandon , Milne Rebecca , Shawyer Andrea , Meenaghan Amy , Ribbers Eva 
Published: July 2022

Abstract
It is predicted that emergency response to critical incident events will likely increase in the coming decades, as society faces unique and dynamic challenges (e.g., pandemics, migrant crises, terrorism). As such, it is necessary to breakdown, identify and evaluate the unique barriers associated with decision-making in the context of critical incident response. Current research has often framed the factors that influence critical incident-related decision-making in respect to independent singular sociological and psychological characteristics. The aim of this study was to synthesise the bibliographic characteristics of the research and present a holistic narrative analysis of the multi-layered factors associated with decision-making. The systematic synthesis of evidence also facilitated a critical appraisal of the quality and distribution of evidence across macro-, meso-, and micro- levels. Results suggested that research was moderately heterogeneous, as the synthesis of evidence captured diverse narrative factors. However, eligible research remained sparse and often focused on singular factors. Further, micro-centric characteristics (e.g., cognitive-related factors) were not well represented. Research primarily focused toward intermediate meso-level characteristics, capturing factors such as ‘interoperability’ and ‘organisation policy and procedure’ as critical challenges to decision-making. Six key narratives were also identified and discussed. Both the quality appraisal and narrative findings suggested that research should seek opportunities to experimentally assess, evaluate and validate decision-making. In practice, whilst this has in the past appeared ethically and practically problematic, advances in technology, research and analysis have allowed high-fidelity simulation experimentation to recreate critical incident events.
Polarizing properties of lipoxin A4 on THP-1 macrophages in vitro
Authors: Aubeux Davy , Tessier Solene , Perez Fabienne , Geoffroy Valerie , Ga Alexis PhD 
Published: July 2022

Abstract
Lipoxin A4 (LXA4) is a specialized pro-resolving mediator involved in the resolution phase of inflammation that is crucial for the return of tissues to homeostasis, healing, and regenerative processes. LXA4 acts via its receptor, formyl peptide receptor 2 (FPR2), to promote macrophage polarization. The objective of this study was to assess the effect of LXA4 on macrophage activity and on the phenotype modulation of polarized M1 and M2 macrophages derived from THP-1 monocytes. Once differentiated, human macrophages were incubated with interleukin 4 (IL-4) and IL-13 to obtain M2-polarized macrophages or with interferon gamma and lipopolysaccharide for classical macrophage activation. The mRNA and protein expression of M1 and M2 markers confirmed the polarization of THP-1-derived macrophages. LXA4 (0-100nM) did not affect the viability of M1 and M2 macrophages or the phagocytic activity of these cells. Gene expression of FPR2, referred as a receptor for the LXA4, was higher in M1 compared with M2, and was not modified by the LXA4 at the doses used. Moreover, LXA4 exhibited anti-inflammatory effects by decreasing the gene expression of pro-inflammatory cytokines (IL-6, tumor necrosis factor alpha, IL-1β) in M1 and increasing the expression of anti-inflammatory cytokines (IL-10) in M2. These results provide new insights regarding the potential of LXA4 to switch the polarization state of macrophages.
The occurrence of primary REE minerals and their paragenesis within S-type granite and quartz vein, South Bangka, Bangka Belitung Islands, Indonesia
Authors: Tampubolon Armin , Syafri Ildrem , Rosana Mega Fatimah , Yuningsih Euis Tintin 
Published: July 2022

Abstract
Rare earth elements (REE) is categorized as a part of critical mineral resources because of their important uses in modern technology and the limited resources that are dominated by China. The indication of primary REE minerals was identified in some localities in South Bangka, Indonesia. Such minerals were investigated using various methods such as petrography, ore microscopy, XRF, ICP-MS/OES, SEM-EDS/EDX, EPMA, fluid inclusion study, and stable isotope O and S analysis. Two types of REE minerals have been classified, i.e., carbonate and phosphate. The carbonate type consists mainly of parisite, allanite, and bastnasite, while REE phosphate group comprises mainly monazite and xenotime. The presence of such REE types is also accompanied by other REE-bearing minerals such as thorite and zircon in both granite and quartz veins. The granite with the highest REE values has a lesser negative anomaly of Eu. In other words, it is a less perfectly fractionation than the others, it belongs to biotite granite. In contrast, REE-bearing quartz vein is associated with cassiterite, pyrite, chalcopyrite, sphalerite, and tourmaline. Based on homogenization temperatures and incorporated with stable isotope O and S analyses, and fluid inclusion study results, the hydrothermal fluid that plays a role in REE mineral precipitation is subdivided into three stages, i.e., early (> 316oC − 316oC), intermediate (< 316oC – 210oC), and late (< 210oC- 190oC). The isotopic analysis shows that the δ18O range of 12,7‰ – 13‰ and δ34S range of 3,9‰ – 5,5‰ indicate that the origin of hydrothermal fluid comes from mixing fluids of both meteoric and magmatic sources, and also influenced by metamorphic waters. While the source of sulfur is granitic and sedimentary rocks. REE phosphate is thought to have formed in the early magmatic crystallization stage except for monazite which is formed in the intermediate magmatic-hydrothermal and late hydrothermal stages. Primary REE carbonate may have formed in an intermediate hydrothermal-magmatic stage except for parasite and allanite, they may have formed in late-stage hydrothermal. Sulfide minerals such as chalcopyrite and sphalerite seem to be associated with REE minerals in the intermediate and late hydrothermal stages.