bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prompting of commercial climate
Authors: Meenal  
Published: February 2025

Abstract

A commonly expressed apprehension is that the advocacy efforts of corporations may impede the effective enforcement of ambitious climate policies. We measure the extent of commercial attitudes towards both mitigating and exacerbating climate change. Examine the prompting expenditures of publicly traded companies in the United States and determine the most prominent commercial advocates along with their underlying motivations. On average, companies allocate approximately $277,000 annually towards prompting efforts aimed at opposing climate-related policies, with a majority of this expenditure totalling $185,000 specifically directed towards supporting initiatives that are against climate regulations/climate prompting). Adversaries of climate action tend to have business models that contribute significantly to carbon emissions, whereas advocates for climate action are more inclined to demonstrate engagement in environmentally friendly innovation. Companies that allocate a higher budget towards initiatives aimed at mitigating climate change.Research suggests that prompting activities may result in higher returns, potentially due to the impact of a risk mechanism. Our findings are consistent with the growing perspective among investors that engaging in anti-climate prompting represents a financial risk.
Therapeutic Effect of Cisplatin-Loaded Polymeric Nanoparticles and Nimotuzumab Combination in Human Glioblastoma Cells
Authors: Kaga Elif , Korkmaz Serhat , Kaga Sadik 
Published: February 2025

Abstract

Objective
This study evaluated the combined therapeutic effects of cisplatin-loaded PEG-PLGA nanoparticles (CIS-PEG-PLGA) and nimotuzumab (NIMO) on glioblastoma (GBM) cells, focusing on their antiproliferative, apoptotic, and cell cycle-modulatory effects.
Materials and Methods
PEG-PLGA nanoparticles were synthesized using a double-emulsion solvent evaporation method, achieving a mean particle size of ~ 450 nm. Cisplatin release kinetics, proliferation, apoptosis, and cell cycle progression were assessed in U87 glioblastoma cells. The treatments included free cisplatin (CIS), CIS-PEG-PLGA, NIMO, and their combinations. Experiments were performed in triplicate, and statistical analyses included ANOVA test.
Results
The CIS-PEG-PLGA nanoparticles exhibited sustained cisplatin release, with 50% released within 8 h and nearly 100% within 72 h. The combination of CIS-PEG-PLGA + NIMO reduced cell proliferation by 90%, significantly surpassing that of CIS (50%) and CIS-PEG-PLGA (70%). Apoptosis rates were highest in the combination group (27.52%), followed by CIS (18.59%) and CIS-PEG-PLGA (25.95%). Cell cycle analysis revealed significant G2 phase arrest (30.12%) in the combination treatment group, highlighting its superior modulatory effects on cell cycle progression.
Conclusion
The combination of CIS-PEG-PLGA and NIMO demonstrated synergistic therapeutic effects in glioblastoma cells, enhancing antiproliferative, apoptotic, and cell cycle-modulatory outcomes. This approach represents a promising advancement in glioblastoma therapy by integrating nanotechnology and immunotherapy to overcome conventional treatment limitations. Further, in vivo studies are warranted to validate these findings and explore their clinical applications.
In silico design and characterization of an ISG75-derived chimeric protein from Trypanosoma evansi: a potential vaccine candidate
Authors: Neves Gabriella Bassi , Moreira Renato Simões , Alves Emanuelle Lemos , Scheffer Eduarda Karolyne , Miletti Luiz Claudio 
Published: February 2025

Abstract

Vaccination remains the most effective practical approach for controlling the spread of infections and alleviating the adverse health effects of diseases. Surra, caused by Trypanosoma evansi, affects various hosts, enabling the disease's continual transmission between its wildlife reservoir and domesticated or working animals. Furthermore, parasites utilize mechanisms such as antigenic variation to circumvent both innate and adaptive immune responses, thereby complicating the development and effectiveness of an anti-trypanosome vaccine. We used an in silico method to design a novel vaccine consisting of a chimeric protein that incorporates predicted epitopes of Invariant Surface Glycoprotein 75 (ISG75) of T. evansi to stimulate an immune response against the parasitic infection. The assessment of predictions was conducted utilizing VaxiJen and ANTIGENpro to facilitate the identification of four epitopes with antigenic properties. In the selected ISG75 sequences, we obtained scores between 0.7 and 1.4 on VaxiJen and between 0.15 and 0.57 with ANTIGENpro. The analysis of immunoinformatics, physicochemical characteristics of the chimeric protein, and molecular simulations indicate that the multiepitope vaccine may be efficacious and appropriate for in vitro evaluation, aiming to develop a peptide vaccine against T. evansi infection. The effectiveness of ISG75 as a vaccine candidate has been validated by multiple previous in silico experimental trials. Consequently, we propose an alternative multiepithope ISG75 vaccine for immunization against T. evansi. No chimeric protein has previously been evaluated against any salivarian trypanosomes.
The Herbicidal Potential of Euphorbia Species for Managing Common Weeds in the Mount Cameroon Region
Authors: Egbe Andrew Enow , Nsanyi Fritz Mukow , Ndam Lawrence Monah , Soupi Nkeutcha Marietta Solange 
Published: February 2025

The utilization of botanicals in weed management is of great significance due to their biodegradability and eco-friendly nature. This research evaluates the herbicidal potentials of seven Euphorbia species (Euphorbia hirta, Euphorbia maculata, Euphorbia heterophylla, Euphorbia prostrata, Euphorbia pulcherrima, Euphorbia trigona, and Euphorbia milii) in the Mount Cameroon Region.The assessment of the herbicidal potential of the most promising Euphorbia species was conducted using the Sandwich method along with germination bioassay techniques. Qualitative phytochemical screening techniques were employed to investigate the herbicidal properties of E. heterophylla through the identification of classes of secondary metabolites. Additional analysis of Faculty of Agriculture and Veterinary Medicine, University of Buea, Cameroon, P.O Box 63, Cameroon. The crude extracts from Euphorbia heterophylla regarding the germination and development of five weed species revealed inhibiting effects on all the weeds examined. The results showed that Euphorbia heterophylla exhibited inhibitory effects on Lactuca sativa seeds, with a maximum inhibition of 85.75% at higher concentrations, indicating dose-dependent trends. Bidens pilosa had the highest herbicidal suppression rate of 83.91%, while Mimosa invisa exhibited the least suppression at 61.10%. Thirteen (13) chromatographic fractions of Euphorbia heterophylla were tested for their influence on the germination and growth of Bidens pilosa seeds. The findings showed that fractions F4, F6, F7, and F13 demonstrated total inhibition of the weed (100% suppression). Phytochemical analysis of E. heterophylla identified the presence of flavonoids, phenolic compounds, alkaloids, tannins, steroids, saponins, and cardiac glycosides. Euphorbia heterophylla, had a strong allelopathic potential, making it viable candidate for the isolation and development of bioactive compounds. These compounds could be utilized as bioherbicides and thereby contributing to sustainable weed management in crop production.
Beyond hydrologic benefits: post-occupancy evaluation of the Sponge City Construction in China, a case study of the Quyuan Park, Changde
Authors: Xu Hanwen , Randall Mark , Liu Yu , Xu Meng , Fryd Ole 
Published: February 2025

ABSTRACT
The Sponge City Construction (SCC) attempts to provide a solution for coping with urban flooding and water-environmental issues in China. During the last decade, numerous SCC projects have been implemented in green spaces (so-called spongy green spaces) which combined hydrological benefits and socio-ecological benefits. Much research attention has been paid to hydrologic modeling as a pre-step of implementation. However, less attention is focused on evaluating and monitoring these projects after implementation. This study used post-occupancy evaluation (POE), combining analytic hierarchy process, fuzzy comprehension evaluation, and semi-structured interview methods to demonstrate the public perception of the Quyuan Park in Changde. The results showed the following: (1) Over 50% of responses for 31 out of 33 indicator questions were “strongly agree”, reflecting an overall positive evaluation; (2) air quality, plant growth, and physical well-being received the three highest satisfaction scores; (3) indicator scores for water management and service management category are relatively low; (4) dissatisfaction and improvements focused on needs for commercial facilities and children's playgrounds. The mismatch between technical objectives and respondent feedback implied a gap between SCC implementation and public awareness. The findings provide a reference and indicator framework of POE for policymakers, planners, and designers in the future.
Knowledge, Attitude, and Practices of Caregivers in the Management of Asthmatic and Non-asthmatic Children: A Community-based Study
Authors: Shaheen Majd , Nishat Syed Muhammad Hayyan , Tanweer Ammar Shahid , Alshamsi Bashayer , Tanweer Ariba Shahid , Nishat Aroob , Jhancy Malay , Khan Karuna 
Published: February 2025

Background:
Bronchial asthma, a chronic inflammatory condition, poses significant health challenges for children. Proper management of childhood asthma
requires the knowledge, attitudes, and practices (KAP) of the caregivers. This study assesses the KAP of caregivers in the management of
asthmatic and non-asthmatic children in the UAE.

Methods:
A cross-sectional study was conducted in Ras Al Khaimah over four months, involving 182 children aged 6-16 years. Data were collected through
a pre-validated KAP questionnaire distributed to caregivers of asthmatic and non-asthmatic children across schools, parks, and private clinics.
Descriptive statistics were used to analyze the responses.

Results:
Among caregivers of asthmatic children, 77.1% demonstrated adequate knowledge, while 45.83% held a positive attitude towards asthma. In
contrast, caregivers of non-asthmatic children had lower knowledge (37%) and attitudes (26.88%). Lack of adequate knowledge led to poor asthma
management practices, with only 75% of caregivers conducting regular follow-ups. Better caregiver knowledge was associated with improved
asthma control, while misconceptions and negative attitudes led to suboptimal care.

Conclusion:
Significant gaps in caregiver KAP were identified, with a need for targeted educational programs to enhance asthma management. Improving
caregiver understanding and attitudes through regular physician visits and educational initiatives is crucial for optimizing asthma outcomes in
children.
Cyclin Dependent Kinases in Antiviral Drug Discovery
Authors: Tarasova Olga , de Azevedo Junior Walter Filgueira 
Published: February 2025

Abstract:
Cyclin-dependent kinases that are responsible for cell cycle control, have
been studied for over 30 years as therapeutic targets for the treatment of cancer and inflammation.
In the past twenty years, their activities in various viral infections have
been investigated in the search of novel therapeutic strategies in the treatment of viral infections.
The interest in evaluating the antiviral activity of cyclin-dependent kinase inhibitors
is closely linked to their role as host factors in viral replication. Due to the development
of viral resistance, the strategies directed toward the targeting host machinery
are still under investigation. This review is dedicated to the analysis of the molecular
mechanisms of viral infection control by cyclin-dependent kinases that may reveal the
potential mechanisms of action for their inhibitors and regulators as antiviral agents. We
also consider recent efforts and achievements in the development of potential antiviral
agents based on the cyclin-dependent kinase inhibitors and regulators, including their effects
on various viruses, side effects, and toxicities.
In vitro Antibiofilm Activity-directed In silico Identification of Natural Products Targeting Bacterial Biofilm Regulators SarA and LasR
Authors: Shilpi Jamil A. , Sarker Dipto Kumer , Seidel Veronique , Ali Mohammad Tuhin , Uddin Shaikh Jamal , Basak Ananya , Chakraborty Shaown , Khairuzzaman M , Nahar Akhlak Un , Salam Fayad Bin Abdus 
Published: February 2025

Background:
Antibiofilm agents serve as an essential tool in the fight against antibiotic
resistance, and natural products provide a promising source for potential drug leads.

Objective:
This study investigates the activity of twenty Bangladeshi medicinal plants against
Staphylococcus aureus and Pseudomonas aeruginosa biofilms and predicts the interactions of
selected phytochemicals from five of the best performing plants with the active sites of transcriptional
regulatory proteins SarA of S. aureus and LasR of P. aeruginosa.

Methods:
The plant extracts were tested by microtiter plate-based assay against S. aureus and P.
aeruginosa biofilms. Molecular docking and molecular dynamics simulation (MD) were conducted
using PyRx and GROMACS, respectively.

Results:
The best activity was identified for Cassia fistula and Ananas comosus, showing ≥ 75% inhibition of biofilm formation. ent-Epicatechin-(4α→8)-epiafzelechin (EEE) of C. fistula, cyanidin-3,3',5-tri-O-β-D-glucopyranoside (CTG) of A. comosus, and 7-O-(4-hydroxy-Ecinnamoyl)- spinoside of A. spinosus showed the best predictive binding affinity (-7.6, -7.6 and - 7.7 kcal/mol, respectively) for SarA. EEE was the only ligand to exhibit a stable ligand-protein complex with SarA in the MD simulation of 200 ns (binding energy of MMPBSA analysis - 39.899 kJ/mol). Chrysophanol, epicatechin and physcion, of C. fistula (-10.5, -10.5, and -11.0 kcal/mol, respectively) and auraptene of F. limonia (-10.8 kcal/mol) showed the best predictive binding affinity for LasR. Epicatechin showed the most stable ligand-protein complex with LasR (binding energy of MMPBSA analysis -63.717 kJ/mol)

Conclusion:
Epicatechin and its derivative EEE could be used as scaffolds for the development
of new antibiofilm agents against P. aeruginosa and S. aureus, respectively.
Design and Characterization of Antibacterial Peptide Nanofibrils as Components of Composites for Biomaterial Applications
Authors: Sawicka Justyna , Bollin Piotr , Sylla Anna , Panasiuk Mirosława , Wilkowska Michalina , Ciołek Lidia , Leśniewski Mateusz , Konopka Aleksandra , Struniawski Karol , Całka-Kuc Gabriela , Liwo Adam , Hańczyc Piotr , Kozak Maciej , Gromadzka Beata , Biernat Monika , Rodziewicz-Motowidło Sylwia 
Published: February 2025

Purpose:
The purpose of this study was to design and synthesize the ug46 peptide, incorporate
its fibrils into composite materials, and evaluate its structural and antimicrobial properties.
Another objective was to utilize spectroscopy and molecular simulation, enhanced by Machine
Vision methods, to monitor the aggregation process of the ug46 peptide and assess its potential
as a scaffold for an antimicrobial peptide.

Method:
The structural analysis of the ug46 peptide reveals its dynamic conformational changes.
Initially, the peptide exhibits a disordered structure with minimal α-helix content, but as incubation
progresses, it aggregates into fibrils rich in β-sheets. This transformation was validated by CD
and ThT assays, which showed decreased molar ellipticity and an increase in ThT fluorescence.

Results:
Laser-induced fluorescence and molecular dynamics simulations further revealed the transition
from a compact native state to extended “worm-like” filament structures, influenced by peptide
concentration and temperature. TEM and AFM confirmed these changes, showing the evolution
of protofibrils into mature fibrils with characteristic twists. When incorporated into chitosan-
bioglass composites, these fibrils significantly enhanced antimicrobial activity against pathogens
such as Staphylococcus aureus and Pseudomonas aeruginosa.

Conclusion:
Overall, ug46 peptide fibrils show promise as a multifunctional scaffold with structural
and antimicrobial benefits in composite biomaterials.
The Modulatory Effects of Anesthetics and Analgesics on Neurophysiological Monitoring and Underlying Mechanisms
Authors: Leng Yu , Teng Yi , Liu Jin , Zou Xian , Ou Mengchan , Zhu Tao , Liang Peng , Zhou Cheng 
Published: February 2025

Abstract:
Intraoperative Neurophysiological Monitoring (IONM) is an indispensable surgical
tool that offers invaluable insights into neurological function across a spectrum of anatomical
areas. By comprehensively assessing the integrity of the brain, brainstem, spinal cord, cranial
nerves, and peripheral nerves, IONM plays a pivotal role in guiding surgical decision-making
and optimizing patient outcomes, particularly in the context of high-risk procedures. Intraoperative
drugs, especially anesthetics and/or analgesics, differentially modulate neurophysiological
monitoring, which remarkably affects the application of neurophysiological monitoring under
specific conditions and indicates the neurobiological mechanisms of anesthetics/analgesics. This
review will describe various neurophysiological modalities utilized in intraoperative procedures,
each employing a wide variety of physiological principles; summarize the modulatory effects of
anesthetics/analgesics on these neurophysiological monitoring parameters; and elucidate their
underlying mechanisms, with a particular emphasis on evoked potentials. Insights gleaned from
this review can inform strategies of anesthesia management for surgeries that require IONM and
guide future investigations on the mechanisms of anesthesia/analgesia.
Dihydromyricetin Improves Myocardial Functioning by Influencing Autophagy Through SNHG17/Mir-34a/SIDT2 Axis
Authors: Xiao Hai , Xiao Yan , Zeng Xueliang , Xie Huihui , Wang Ziyao , Guo Yu 
Published: February 2025

Background:
Diabetic cardiomyopathy [DCM] is a common and severe complication of Diabetes Mellitus [DM]. Dihydromyricetin [DHM] is a flavonoid
compound with potential cardioprotective effects, but the mechanism of DHM in diabetes-induced myocardial damage and autophagy is not fully
understood.

Objective:
The objective of this study is to evaluate the effects of DHM on cardiac function and pathological features of DCM, with a particular focus on its
impact on the SNHG17/miR-34a/SIDT2 pathway.

Methods:
In vivo experiments: After constructing the DM mice model, it was treated with different doses of DHM. Masson's staining and collagen
deposition/fibrosis markers were used to evaluate the effect of DHM on cardiac fibrosis in DM mice. In vitro experiments: 3-[4,5-
dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide [MTT] assay and flow cytometry were used to determine the influence of DHM on cell
viability and apoptosis, respectively, in high glucose-induced HL-1 cells. ELISA was used to detect levels of cardiac enzyme and inflammationrelated
factors, while Western blot analyzed the levels of AMPK/mTOR and autophagy-related proteins.

Results:
DHM significantly improved cardiac function in DM and reduced Renin-angiotensin-aldosterone system markers, alongside decreasing markers of
cardiomyocyte damage. DHM mitigated myocardial fibrosis, inflammatory marker levels, and autophagy dysregulation while upregulating
lncRNA SNHG17 expression. Mechanistically, DHM acted through the SNHG17/miR-34a/SID1 transmembrane family member 2 [SIDT2] axis,
reducing miR-34a expression and restoring SIDT2-mediated autophagy balance, ultimately alleviating apoptosis, inflammation, and fibrosis in
diabetic cardiac tissue and high-glucose-induced HL-1 cells.

Conclusion:
DHM improves cardiac function and mitigates DCM progression by targeting the SNHG17/miR-34a/SIDT2 regulatory axis, thereby reducing
inflammation, fibrosis, and autophagy dysregulation. These findings provide mechanistic insights into DHM’s cardioprotective effects, supporting
its potential as a therapeutic agent for DCM.
Exploring the Immune-Related Molecular Mechanisms Underlying the Comorbidity of Temporal Lobe Epilepsy and Major Depressive Disorder through Integrated Data Set Analysis
Authors: Yan Shi , Han Zhibin , Wang Tianyu , Wang Aowen , Liu Feng , Yu Shengkun , Xu Lin , Shen Hong , Liu Li , Lin Zhiguo , Na Meng 
Published: February 2025

Background:
Temporal lobe epilepsy (TLE) and major depressive disorder (MDD) are prevalent and complex neurological disorders that affect individuals
globally. Clinical and epidemiological studies indicate a significant comorbidity between TLE and MDD; however, the shared molecular
mechanisms underlying this relationship remain unclear. This study aims to explore the common key genes associated with TLE and MDD through
a systematic analysis of gene expression profiles, elucidate their underlying molecular pathological mechanisms, and evaluate the potential
applications of these genes in diagnostic and therapeutic contexts.

Methods:
Brain tissue gene expression data for TLE and MDD were obtained from the GEO database. Differentially expressed genes (DEGs), weighted gene
co-expression network analysis (WGCNA), functional enrichment, and protein-protein interaction (PPI) network construction were performed to
identify shared gene modules. LASSO and random forest (RF) machine learning models were used to select diagnostic candidate genes, validated
through ROC curve analysis. Immune infiltration analysis explored the immune involvement of key genes, while single-cell sequencing confirmed
gene expression across cell types. Potential therapeutic drugs were identified using a drug database.

Results:
A total of 372 DEGs were identified as either up- or down-regulated between TLE and MDD, with WGCNA revealing nine shared gene modules.
Seven hub genes, including HTR7 and CDHR2, demonstrated strong ROC performance. Immune infiltration analysis revealed changes in immune
cell populations linked to key genes, confirmed by single-cell sequencing. Upadacitinib was identified as a potential therapeutic drug targeting
these genes.

Conclusion:
This study identified shared gene expression profiles between TLE and MDD, emphasizing immune pathway-related molecular mechanisms.
Immune infiltration analysis and single-cell sequencing underscored the significance of immune regulation in their comorbidity, while drug
prediction highlights candidates for precision medicine, establishing a foundation for future research and therapeutic strategies.