bluuebunny/crossref_metadata_2025_split huggingface dataset:
YAP/TAZ reverse progestin resistance through PI3K-Akt pathway in endometrial carcinoma
Authors: Wei Lina , Ma Xiaohong , Hou Yixin , Zhao Tianyi , Sun Rui , Qiu Chunping , Liu Yao , Qiu Ziyi , Jiang Jie 
Published: August 2022

AbstractBackground:Progestin resistance is a problem for patients with endometrial carcinoma (EC) who require conservative treatment with progestin, and its underlying mechanisms remain unclear. YAP and TAZ (YAP/TAZ), downstream transcription coactivators of Hippo pathway, are highly expressed and induce aggressive phenotypes of EC. However, the role of YAP/TAZ in progestin resistance of EC is still unknown. The aim of this study is to explore the functions and underlying mechanisms of YAP/TAZ in progestin resistance of EC.Methods:The expression of YAP/TAZ in progestin resistant tissues was examined by immunohistochemistry. We developed a stable medroxyprogesterone acetate (MPA) resistant cell line (IshikawaPR cell). We knocked down YAP/TAZ in IshikawaPR cell, and overexpressed them in progestin sensitive Ishikawa cell for further studies. The proliferative capacity of cell was investigated by MTT, EDU and colony formation assay. Flow cytometry was used to detect the change of cell apoptosis. Transwell and wound healing assay were used to determine the change of cell metastasis ability after different concentrations of MPA treatment. The tumor xenograft experiment was performed to prove the role of YAP/TAZ inhibitor Verteporfin in progestin treatment. The next-generation sequencing (NGS) and rescue experiments were carried out to identify the mechanism involved in the process that YAP/TAZ promote progestin resistance.Results:The expression of YAP/TAZ increased remarkably in progestin resistant tissues. YAP/TAZ were upregutated in IshikawaPR cell versus Ishikawa cell. Knocking down YAP/TAZ decreased cell viability, inhibited cell migration and invasion and increased the sensitivity of IshikawaPR cell to progestin. Overexpression of YAP/TAZ conversely increased cell proliferation, metastasis and promoted progestin resistance. Verteporfin could increase sensitivity of IshikawaPR cell to progestinin vivo and in vitro.Results of the NGS revealed that knocking down YAP/TAZ inhibited PI3K-Akt pathway, which was testified by western blot. The rescue experiments further confirmed overexpression of YAP/TAZ might take part in progestin resistance of EC by upregulating PI3K-Akt pathway.Conclusions:YAP/TAZ as well as Verteporfin could reverse progestin resistance by regulating PI3K-Akt pathway in EC, which may provide ideas for clinical targeted therapy of progestin resistance.
Construction of Heterogeneous Biological Networks and Its Application
Authors: Zhang Zhihong , Luo Yingchun , Zhao Bihai , Xiong Huijun , Yan Wei , Jiang Meiping , Hu Sai 
Published: August 2022

Abstract
Background: Accurate annotation of protein function is the key to understanding life at the molecular level and has great implications for biomedicine and pharmaceuticals. The rapid developments of high-throughput technologies have generated huge amounts of protein-protein interaction (PPI) data, which prompts the emergence of computational methods to determine protein function. Plagued by errors and noises hidden in PPI data, these computational methods have undertaken to focus on the prediction of functions by integrating the topology of protein interaction networks and multi-source biological data. Despite effective improvement of these computational methods, it is still challenging to build a suitable network model for integrating multi-omics data. ResultsIn this paper, we constructed a heterogeneous biological network by initially integrating original protein interaction networks, protein-domain association data and protein complexes. To prove the effectiveness of the heterogeneous biological network, we applied the propagation algorithm on this network, and proposed a novel iterative model, named PHN (Propagate on Heterogeneous Biological Networks) to score and rank functions in descending order from all functional partners and selected the first L of them as candidates to annotate the target protein. Our comprehensive experimental results demonstrated that PHN outperformed six other competing approaches using cross validation. Experimental results indicated that PHN performs significantly better than competing methods and improves the AUROC (Area Under the Receiver-Operating Curve) by no less than 32%.Conclusions:We demonstrated that integrating multi-source data into a heterogeneous biological network can preserve the complex relationship among multi-omics data and improve the prediction accuracy of protein function by getting rid of the the constraints of errors in PPI networks effectively. PHN, our proposed method, is effective for protein function prediction.
The Effect of Whole Body Massage on Pain Scores of Neonates During Venous Puncture and Comparison With Oral Dextrose and Kangaroo Care, a Randomized Controlled Evaluator-blind Clinical Study
Authors: Yıldızdaş Hacer Yapıcıoglu , erdem Buket , Karahan Duygu Yıldız , OZLU FERDA , Sertdemir Yaşar 
Published: August 2022

Abstract
Objective: Newborns in NICUs experience many painful procedures. The aim of the study was to evaluate the effect of whole body massage therapy on pain scores during venipuncture and compare with oral 10% dextrose and Kangaroo care. Study Design: Newborns with gestational age ≥34 weeks were randomly enrolled to one of three groups: massage, dextrose and Kangaroo and a blinded investigator scored the pain using NIPS before and during the procedure. Results: There were 25, 26 and 23 newborns in dextrose, massage and Kangaroo care groups, respectively. Pain scores were similar before and during venipuncture in groups (p> 0.05). 36.5% of newborns (27/74) had severe pain scores. Number of newborns with no pain (score 0-2), moderate pain (score 3-4) and severe pain (score 5-7) were similar in each group. Conclusion: Massage, Kangaroo and oral 10% dextrose had similar effects on pain scores during venipuncture.
Design and Analysis of Tri-bandsTriangular Shape Fractal Antenna for Wireless Applications
Authors: raj arun , Dhubhakarya D.C. , Srivas D.K. , Tiwari Manoj K. 
Published: August 2022

Abstract
In this paper fractal antenna is proposed for wide band applications. Proposed Antenna is having resonance frequency of 5 GHz and it has triangular patch with 36mm base and 36mm height. This antenna design is used to obtain multiband behavior and wide band characteristics by which antenna can be cover various applications in wireless communication. Antenna has been made with 4 iterations and the result obtained for the proposed antenna is in agreement for the various efficient wireless applications. The fractal antenna here described, is basically the Sierpinski gasket with fragmented geometrical shape with various subdivisions. The proposed antenna has been designed using FR4 substrate with width and length of 4cm. In Proposed Antenna, there are three bands at frequencies 4.8-5.9 GHz, 7.1-7.4 GHz, 8.2-8.9 GHz and bandwidth percentages are 20.56%, 4.13% and 8.19%. And in final iteration there are tri bands and our proposed fractal antenna cover applications in 4.8-5.9 GHz, 7.1-7.4 GHz, 8.2-8.9 GHz. The simulated gain of antenna is 7.5dBi and the directivity of proposed antenna is simulated as 7.422 dBi. The proposed antenna has been implemented successfully using IE3D and the result has been tested successfully using vector network analyzer.
Keywords: Fractals; Fractal Antenna; Sierpinski Gasket Fractal Antenna
Risk factors for ventilator-associated pneumonia due to multi-drug resistant organisms after cardiac surgery in adults
Authors: Wang Meizhu , Xu Xi , Wu Shuo , Sun Huiying , Chang Yan , Li Meng , Zhang Xiaoxiao , Lv Xing , Yang Zhaoxu , Ti Xinyu 
Published: August 2022

Abstract
Background: Ventilator-associated pneumonia (VAP) is one of the most common intensive care unit (ICU)-acquired infections，which can cause multiple adverse events. Due to bacterial mutation and overuse of antimicrobial drugs, multidrug-resistant organisms (MDRO) have become an important group of VAP infections in postoperative cardiac patients. Therefore, the purpose of this study is to explore the risk factors for VAP with MDRO following cardiac surgery in adults. Methods: The clinical data of adult patients with VAP following cardiac surgery in the hospital from Jan 2017 to May 2021 were analyzed retrospectively, the patients were divided into the MDRO VAP group and the non-MDRO VAP group. Univariable and multivariable logistic regression analyses were performed for the risk factor for the patient with MDRO VAP. The species and drug sensitivity of pathogens isolated from the VAP patients were also analyzed. Results: A total of 61 VAP cases with 34 cases in the MDRO VAP group (55.7%) and 27 cases in the non-MDRO VAP group (44.3%) were involved in this study. Multivariable logistic regression analysis showed that the independent risk factors for the MDRO VAP included preoperative creatinine clearance rate (CCR) ≥86.6ml, intraoperative cardiopulmonary bypass (CPB) time≥151 mins, postoperative acute kidney injury (AKI) and nasal feeding. Gram-negative bacilli were the main pathogens in VAP patients (n=54, 90.0%), with the highest rate of Acinetobacter baumannii (n=24, 40.0%). Patients with MDRO VAP had significantly longer postoperative intensive care unit (ICU) duration and higher hospitalization costs than non-MDRO VAP patients, but there was no significant difference in the 28-day mortality rate between the two groups. Conclusion: Based on implementing measures to prevent VAP, clinicians should pay more attention to patients with kidney disease, intraoperative longer CPB time, and postoperative nasal feeding to avoid MDRO infections.
Targeting PP2A-dependent autophagy enhances sensitivity to ruxolitinib in JAK2V617F myeloproliferative neoplasms
Authors: Courdy Charly , De Araujo Isabelle , Sahal Ambrine , Ducau Charlotte , Saland Estelle , Edmond Valerie , Tavitian Suzanne , Bertoli Sarah , Cougoul Pierre , Poillet Laura , Plo Isabelle , Sarry Jean-Emmanuel , manenti stephane , De Mas Véronique , Joffre Carine 
Published: August 2022

Abstract
The Janus kinase 2 (JAK2)-driven myeloproliferative neoplasms (MPNs) are chronic malignancies associated with high risk of complications and suboptimal responses to JAK inhibitors such as ruxolitinib. Therefore, better understanding cellular changes induced by ruxolitinib is required to develop new combinatory therapies to improve treatment efficacy. Here, we demonstrated that ruxolitinib induced autophagy in JAK2V617F cell lines and primary MPN patient cells through the activation of protein phosphatase 2A (PP2A). Inhibition of autophagy or PP2A activity along with ruxolitinib treatment reduced proliferation and increased death of JAK2V617F cells. Accordingly, proliferation and clonogenic potential of JAK2V617F-driven primary MPN patient cells, but not of normal hematopoietic cells, were markedly impaired by ruxolitinib treatment with autophagy inhibitor or PP2A inhibitor. Finally, preventing ruxolitinib-induced autophagy with a novel potent autophagy inhibitor Lys05 improved leukemia burden reduction and significantly prolonged mice overall survival compared with ruxolitinib alone. This study demonstrated that PP2A-dependent autophagy mediated by JAK2 activity inhibition contributed to the resistance to ruxolitinib. Altogether, our data support that targeting autophagy or its newly identified regulator PP2A could enhance sensitivity to ruxolitinib of JAK2V617F MPN cells and improve MPN patient care.
Effect of the Accession to the World Trade Organization on Commercial Services Exports
Authors: GNANGNON Sèna Kimm 
Published: August 2022

Abstract

The literature on the trade effects of the membership in the World Trade Organization (WTO) has essentially focused on trade in goods, including goods exports. The present analysis has investigated the effect of the WTO membership on commercial services exports for 25 Article XII Members (i.e., countries that joined the WTO under Article XII of the Marrakesh Agreement establishing the WTO) over the period from 2005 to 2019. Results, based on the two-stage probit least squares estimator have revealed that the WTO membership increased significantly commercial services exports, including both modern and traditional services exports in Article XII members. The analysis has also used the two-step system generalized method of moments to investigate whether the WTO membership helped Article XII Members better perform than Non-Article XII Members in terms of commercial services exports, given that they undertook higher commitments under the General Agreement on Trade in Services than Non-Article XII Members. The findings have indicated that thanks to the WTO membership, relatively advanced countries among Article XII Members enjoyed both a higher commercial services export performance (including modern services export performance) and a higher degree of services export diversification than Non-Article XII Members. However their traditional services export performance remained, on average, the same as that of Non-Article XII Members. Less advanced economies among Article XII Members performed less than Non-Article XII Members in terms of commercial services exports, including modern and services exports, but they performed better than Non-Article XII Members in terms of traditional services exports. Meanwhile, their membership in the WTO did not result in a significant change in their level of services export concentration compared to that of Non-Article XII Members.
Novel constructed dark, bright, and rogue waves of three models of the well-known nonlinear Schrödinger equation
Authors: Khater Mostafa M. A. 
Published: August 2022

Abstract
In this study, three models of the nonlinear Schrödinger equation are investigated to see whether there is any novel traveling wave solutions’ structures. “The generalized Korteweg-de Vries, (2+1) dimensional Ablowitz-Kaup-Newell-Segur, and the Maccari models ”are among the systems examined. The kinetic energy operator is impacted by mass’s location and time dependence, and different representations of the kinetic energy operator in general. The innovative soliton wave solutions for the studied models are built using the generalized Riccati expansion technique. Some unique figures represent the numerical simulations of the derived solutions. Checking for accuracy and re-entering results into the models is part of validating and improving these approaches (Mathematica 13.1).
Echocardiographic follow-up after cardiac rehabilitation designed for patients with obesity
Authors: Snelder Sanne Marjolein , Uijl Iris , Sunamura Madoka , Zijlstra Felix , Hoeve Nienke ter , Dalen Bas Martijn 
Published: August 2022

Abstract
Aim
We hypothesized that a novel state of the art cardiac rehabilitation (CR) program designed for patients with obesity (OPTICARE XL CR) has better outcomes as compared to standard CR regarding parameters of cardiac function as measured by conventional and advanced transthoracic echocardiography.
Methods
OPTICARE XL CR is an open-label, randomized controlled trial. Inclusion criteria were: patients referred to CR with a body mass index (BMI) ≥ 30 kg/m2, and age ≥ 18 years with either coronary artery disease or nonvalvular atrial fibrillation. The experimental group participated in the novel one year OPTICARE XL CR and the controls received the 6–12 weeks multi-disciplinary exercise-based standard CR program. Subjects randomized to OPTICARE XL CR underwent behavioural therapy for a healthy diet and an active lifestyle for the first 12 weeks. Also, the exercise program was designed for patients with obesity. Furthermore, a behavioural after-care program was organized with 6 meetings between weeks 13–52. Transthoracic (speckle tracking) echocardiography was performed at baseline and one-year follow-up.
Results
A total of 42 patients completed the follow-up, 21 in both groups. There was a mild but statistically significant reduction in weight over time, however, this was comparable between groups. There was no improvement observed in any of the echocardiographic parameters.
Conclusion
Cardiac function in patients with obesity did not improve one-year after a novel state of the art CR program (OPTICARE XL CR) as compared to standard CR.
Transplacental Transfer of Total Immunoglobulin G and Antibodies to Plasmodium falciparum Antigens between the 24th Week of Gestation and Term
Authors: Kayatani Alexander K. K. , Leke Rose G. F. , Leke Robert I. J. , Fogako Josephine , Taylor Diane Wallace 
Published: August 2022

Abstract
Full-term newborns have antibody (Ab) repertoires and levels similar to their mothers to help protect them from environmental pathogens. Unfortunately, preterm babies, especially those born < 34 weeks, have reduced levels of protective antibodies. In Africa, antibodies to Plasmodium falciparum are important in protection from malaria. This study investigated the transfer of total IgG and antibodies to 9 P. falciparum antigens and tetanus toxoid between 24 weeks and term. Paired maternal and cord samples from 166 preterm (24–37 weeks) and 154 term deliveries were used. Transfer efficiency was expressed as the ratio of Ab levels in cord to maternal plasma (CMR). At 24–25 weeks, CMR ranged from 0.31 to 0.94 for the different antigens; the rate of transfer was similar for all antigens between 24–40 weeks; resulting in median CMR of 0.49 to 0.95 at term. Babies of mothers with hypergammaglobulinemia and normal IgG levels had similar amounts of IgG, supporting data that saturation of the neonatal Fc-receptor occurs at ~ 16 mg IgG/ml. Thus, babies born prior to 34–35 weeks in Africa are likely to have reduced Ab levels to some, but not all antigens. Since IgG transfer is Fc-mediated, why differences exist in CMR among the antigens warrants further investigation.
Comparative transcriptomic analysis of early fruit development in eggplant (Solanum melongena L.) and functional characterization of SmOVATE5
Authors: Shi Suli , Li Dalu , Li Shaohang , Wang Yingying , Tang Xin , Liu Yang , Ge Haiyan , Chen Huoying 
Published: August 2022

Abstract
Eggplant, a solanaceous crop that has undergone a long period of domestication, is one of the most important vegetables worldwide. The shape of its fruit is an important agronomic trait and consumers in different regions have different preferences. However, a limited understanding of the molecular mechanisms regulating fruit development and shape has hindered eggplant breeding. In this study, we performed morphological observations and transcriptome analysis of long- and round-fruited eggplant genotypes to understand the molecular regulation during the early development of different fruit shapes. Morphological studies revealed that the two varieties already exhibited distinctly different phenotypes at the initial stage of fruit development before flowering, with rapid fruit enlargement beginning on the sixth day after flowering. Comparative transcriptome analysis identified phytohormone-related genes that were significantly upregulated on the day of flowering, indicating they may be involved in regulating the initial stages of fruit development. Notably, SmARF1 showed a sustained upregulation pattern in both varieties, suggesting that it may promote eggplant fruit growth. In addition, several differentially expressed genes of the SUN, YABBY, and OVATE families are potentially involved in the regulation of fruit development or fruit shape. We demonstrated that the SmOVATE5 gene has a negative regulatory function suppressing plant growth and development. In conclusion, this study provides new insights into the molecular regulatory mechanisms of eggplant fruit development, and the genes identified may provide valuable references for different fruit shape breeding programs.
Muscular Strength Predicts Phase Angle in Breast Cancer Survivors
Authors: SHORT TREVOR , TERANISHI-HASHIMOTO CHERI , YAMADA PAULETTE 
Published: August 2022

Abstract
Phase angle (PhA) has emerged as a prognostic indicator of survival and quality of life (QOL) in cancer patients. Identifying measures of physical fitness that correlate with PhA can provide guidance towards optimizing cancer rehabilitation programs.PURPOSE: To examine the relationship between PhA and physical fitness in breast cancer survivors. METHODS: Sixty-three breast cancer survivors (60 ± 9 years, PhA 4.59±0.52, mean±SD) completed assessments for muscular strength, muscular endurance, cardiorespiratory endurance, flexibility, and body composition. PhA and body composition were measured using bioimpedance analysis (Inbody 770) at 50 KHz. The correlations between phase angle and measures of fitness were evaluated using Pearson coefficients. Simple and multiple linear regression was used to test if measures of muscular strength, muscular endurance, and cardiorespiratory endurance significantly predict PhA.RESULTS: Linear regression analysis showed that incline bench press 1-RM alone explains 28% (r2 = 0.28) of the variance in PhA. Multiple linear regression showed that incline bench press 1-RM, chair squat test repetitions, and predicted VO2peak explain 32% (r2 =0.32) of the variance in PhA. Incline bench press-1RM remained a significant predictor of PhA after adjusting for age (p=0.0001), while muscular endurance and cardiorespiratory endurance did not.CONCLUSION: Muscular strength is suggested to be a significant predictor of PhA in breast cancer survivors, while muscular endurance and cardiorespiratory endurance are not.IMPLICATIONS FOR CANCER SURVIVORS: The prioritization of muscular strength for improving PhA in exercise based cancer rehabilitation programs may be of importance.
Effect of dapagliflozin on the prognosis of patients with acute myocardial infarction undergoing percutaneous coronary intervention
Authors: Zhu Yi , Zhang Jia-li , Yan Xue-jiao , Sun Ling , Wang Fang-fang , Ji Yuan 
Published: August 2022

Abstract
Background and aims: The effect of dapagliflozin (DAPA) on the prognosis of patients with acute myocardial infarction (AMI) is unclear. The present study was conducted to evaluate the association between DAPA administration and adverse events in patients with AMI undergoing percutaneous coronary intervention (PCI).Methods: This single-center retrospective analysis study included a total of 786 patients with AMI from January 2019 to August 2021 who were or were not administered DAPA at discharge. The primary endpoint was the composite of major adverse cardiovascular events (MACE), including overall deaths, heart failure, nonfatal MI, nonfatal stroke, and unplanned repeat revascularization (URR). Differences in the triglyceride glucose (TyG) index and the atherogenic index of plasma (AIP) both during hospitalization and 12 months after discharge (if achievable) were also compared.Results: During a median follow-up of 23 months, 130 patients had MACE (118 in the DAPA-free group and 12 in the DAPA group). Kaplan-Meier survival analyses revealed that the cumulative incidence of MACE (log-rank test, p = 0.009), heart failure (p = 0.003), nonfatal MI (p = 0.005), and URR (p = 0.031) was higher in the DAPA-free group. In addition, the multivariate Cox analysis showed that DAPA was significantly associated with the reduced risk of MACE (hazard ratio = 0.170, 95% confidence interval = 0.078–0.373, p < 0.001). Considering each specific adverse event, the DAPA-free group was associated with heart failure, nonfatal MI, and URR in multivariate Cox regression analyses. Stratification analyses suggested that DAPA has a strong protective effect in patients with AMI of advanced age with concomitant diabetes or those who are not on angiotensin receptor enkephalinase inhibitors. Furthermore, the TyG index and AIP of the patients 12 months after DAPA administration at discharge were significantly lower than those during hospitalization.Conclusions: DAPA is an independent protective factor against MACE and may provide incremental prognostic information in patients with AMI undergoing PCI.