bluuebunny/crossref_metadata_2025_split huggingface dataset:
Malaria Box Compounds against Anopheles gambiae (Diptera: Culicidae) Carboxypeptidase B Activity to Block Malaria Transmission
Authors: Pomun Tippawan , Wonginta Phattaradanai , Kubera Anchanee 
Published: May 2022

Abstract
Carboxypeptidase B (CPB) plays an important role in blood digestion in mosquitos, aiding the release of free amino acids. Anopheles CPB is a target to block malaria transmission because it facilitates Plasmodium invasion of the mosquito midgut. Our study aimed to discover inhibitors of Anopheles CPB to prevent Plasmodium development in the mosquito. The Anopheles gambiae cpb (Agcpb) gene without a signal sequence was cloned into the pET28b expression vector. The recombinant AgCPB protein was expressed in E. coli BL21(DE3) within inclusion bodies after induction with 0.5 mM isopropyl β-D-1-thiogalactopyranoside at 37°C for 4 h. The protein pellet was dissolved in 6 M urea, purified by affinity chromatography, and dialyzed in reaction buffer. The refolded recombinant AgCPB could digest the hippuryl-arginine substrate similarly to that of the commercial porcine pancreas CPB. The 20 top-scoring malaria box compounds from the virtual-screening results were then chosen for an in vitro inhibition assay against AgCPB. Four of the 20 malaria box compounds could inhibit AgCPB activity. The compound MMV007591 was the most potent inhibitor with an IC50 at 0.066 µM. The results indicate that these candidate compounds may be utilized in drug development against mosquito CPB activity to curb malaria transmission.
Benign pituitary adenoma with multiple intracranial metastases—a case report and review of the literature
Authors: Hani Ummey , Tariq Quratulain , Bari Amman , Anis Saad Bin , Diwan Asad 
Published: May 2022

Abstract
Metastatic benign pituitary adenomas (PAs), also known as pituitary carcinomas (PCs), represent 0.1–0.2% of all intracranial lesions. They are rare and challenging pathologies. We present the case of a 34-year-old female, who presented to the clinic with headache and visual loss. She was diagnosed with PA with multiple extra-axial metastases. Debulking and biopsy of the lesions was done. Postoperatively, there was gross residual disease, and the patient’s visual disturbances did not improve. Only 165 cases of PCs have been reported in the current literature. Existing consensus on management of these uncommon lesions is based on trial and previously published case reports and surgery appears to be the only definitive treatment. Further research regarding any non-surgical expectant management is warranted.
Traumatic inferior lumbar hernia
Authors: Baig Niyaz , Elberm Hassan , Warren Patrick 
Published: May 2022

Abstract
Traumatic lumbar hernias are rare, with little surgical literature describing their management. In this we report about a 41-year-old patient admitted to University Hospital Southampton following a high velocity road traffic collision, of estimated speed 140 mph. He suffered multiple bony injuries, and a traumatic right inferior lumbar hernia containing the ascending colon. The hernia was surgically repaired extra-peritoneally with mesh and abdominal wall muscles approximated and sutured.
Popolo and Sindacato in the City of Siena: Rethinking Popular Agency in Medieval Italy
Authors: Martín Romera María Ángeles 
Published: May 2022

Abstract
The Italian sindacato was a procedure that from the end of the twelfth century held major officials accountable and exposed them to public control. While the sindacato presented citizens with the opportunity of bringing forward their claims against officials, the evidence of these claims and their outcomes are sketchy. Recent historiography often portrays this procedure as a rhetorical device of civic order and legitimation that only produced real consequences when used as a tool for the political purposes of the elite. The article focuses on Siena, often singled out for the popular character of its governments, as a unique case to test the actual popular participation and oligarchical involvement in sindacati and their significance in relation to the sindacati in other communal cities. First, it analyzes the wide spectrum of participants in the procedure and, in particular, the interests at play for common actors. Second, it explores the direct relationship between sindacati and other institutions and systems of accountability within the city, as well as their interdependent transformations over time. The result is a revision of sindacati that reintegrates the agency and participation of popular actors. The sindacato appears then as neither a zenith of communal democracy nor a crooked tool in the hands of oligarchical interests, but rather as a central act of civic life articulating a wide range of interests among all sorts of citizens, which allowed for its symbolic relevance to perdure until the Renaissance and beyond.
Zinc Finger-Homeodomain and Mini Zinc Finger proteins are key players in plant growth and responses to environmental stresses
Authors: Bollier Norbert , Gonzalez Nathalie , Chevalier Christian , Hernould Michel 
Published: May 2022

Abstract
The ZINC FINGER-HOMEODOMAIN (ZHD) protein family is a plant-specific family of transcription factors containing two conserved motifs: a non-canonical C5H3 zinc finger domain (ZF) and a DNA-binding homeodomain (HD). The MINI ZINC FINGER (MIF) proteins belong to this family, but were possibly derived from the ZHDs by losing the HD. Information regarding the function of ZHD and MIF proteins is scarce. However, different studies have shown that ZHD/MIF proteins play important roles not only in plant growth and development, but also in response to environmental stresses, including drought and pathogen attack. Here we review recent advances relative to ZHD/MIF functions in multiple species, to provide new insights into the diverse roles of these transcription factors in plants. Their mechanism of action in relation to their ability to interact with other proteins and DNA is also discussed. We then propose directions for future studies to understand better their important roles and pinpoint strategies for potential applications in crop improvement.
Identification of stellar-mass black hole binaries and the validity of linear orbital motion approximation in microlensing
Authors: Ma Xiaoyi , Zhu Wei , Yang Hongjing 
Published: May 2022

ABSTRACT
Gravitational microlensing is unique in detecting binary black (BH) holes with wide (a few au) separations. Models predict that about 1 per cent of microlensing binaries should be due to binary BHs, and yet zero has been robustly identified. Using simulated events with binary BH lenses, we show that the microlensing parallax effect in a typical binary BH event cannot be reliably detected. Given the crucial role of the parallax parameter in determining the mass of dark microlenses, this may explain the non-detection of binary BHs. Additionally, we show that in only a small fraction ($\lesssim 7\ \rm{per\ cent}$) of the simulated events the full orbital motion of the binary lens cannot be modelled with the linear orbital motion approximation. This approximation has been frequently used in modellings of binary microlensing events.
A survey of high-z galaxies: SERRA simulations
Authors: Pallottini A , Ferrara A , Gallerani S , Behrens C , Kohandel M , Carniani S , Vallini L , Salvadori S , Gelli V , Sommovigo L , D’Odorico V , Di Mascia F , Pizzati E 
Published: May 2022

Abstract
We introduce SERRA, a suite of zoom-in high-resolution (1.2 × 104 M⊙, ≃ 25 pc at z = 7.7) cosmological simulations including non-equilibrium chemistry and on-the-fly radiative transfer. The outputs are post-processed to derive galaxy UV+FIR continuum and emission line properties. Results are compared with available multi-wavelength data to constrain the physical properties (e.g. star formation rates, stellar/gas/dust mass, metallicity) of high-redshift 6 ≲ z ≲ 15 galaxies. This flagship paper focuses on the z = 7.7 sub-sample, including 202 galaxies with stellar mass 107 M⊙ ≲ M⋆ ≲ 5 × 1010 M⊙, and specific star formation ranging from sSFR ∼ 100 Gyr−1 in young, low-mass galaxies to ∼10 Gyr−1 for older, massive ones. At this redshift, serra galaxies are typically bursty, i.e. they are located above the Schmidt-Kennicutt relation by a factor $\kappa _s = 3.03^{+4.9}_{-1.8}$, consistent with recent findings for [O iii] and [C ii] emitters at high-z. They also show relatively large IRX =LFIR/LUV values as a result of their compact/clumpy morphology effectively blocking the stellar UV luminosity. Note that this conclusion might be affected by insufficient spatial resolution at the molecular cloud level. We confirm that early galaxies lie on the standard [C ii]$-\rm SFR$ relation; their observed L[OIII]/L[CII] ≃ 1 − 10 ratios can be reproduced by a part of the SERRA galaxies without the need of a top-heavy IMF and/or anomalous C/O abundances. [O i] line intensities are similar to local ones, making ALMA high-z detections challenging but feasible ($\sim 6\, \rm hr$ for an SFR of 50  M⊙ yr−1).
Innovative virtual care delivery in a Canadian paediatric tertiary-care centre
Authors: Goldbloom Ellen B , Buba Melanie , Bhatt Maala , Suntharalingam Sinthuja , King W James 
Published: May 2022

Abstract
Health care systems and providers have rapidly adapted to virtual care delivery during this unprecedented time. Clinical programs initiated a variety of virtual care delivery models to maintain access to care, preserve personal protective equipment, and minimize infectious disease spread. Herein, we first describe the context within paediatric health delivery during the COVID-19 pandemic in Canada that fueled the rise of virtual care delivery. We then summarize the development, implementation, and beneficial impact of the innovative virtual care delivery programs currently in use at Children’s Hospital of Eastern Ontario (CHEO) for both inpatient and outpatient care, specifically in our ambulatory clinics, emergency department, and mental health program. We highlight the transferable unique ways CHEO has integrated virtual care delivery through our governance structure, stakeholder engagement including patient, caregivers and health care providers and staff, development, and use of eHealth tools and novel approaches for patient care requiring physical assessment. We conclude with our vision for the future of virtual care, one component of paediatric care delivery in the post-COVID-19 era, which requires a common framework for virtual care evaluation.
Importantly, rapid implementation of a primarily virtual care model at CHEO sustained high volume quality paediatric care. We believe many of these programs should and will remain in the post-pandemic era. A comprehensive, unified approach to evaluation is essential to yield meaningful results that inform sustainable care delivery models that integrate virtual care, and ultimately help ensure the best health outcomes for our patients.
Exploring Intra-State Tensions in Government Responses to COVID-19
Authors: Federman Peter Stanley , Curley Cali 
Published: May 2022

Abstract
Research and attention to federalism has vastly increased due to the COVID-19 pandemic. While political polarization has largely been used as a scapegoat to explain the U.S. policy response to the pandemic, federalism has also been credited and blamed for the policy response and has played an important role in providing avenues for conflict. This article explores intra-state conflict stemming from COVID-19. We utilize ten exploratory cases to identify three distinct but interrelated patterns of conflict that emerged within U.S. states, focusing on tensions between the executive and legislative branches, between bureaucratic officials and the legislature, and between state and local governments. We then examine a series of questions regarding the implication of these conflicts, focusing on the issuance of executive orders, the responses undertaken by officials who disagreed with and sought to push back against these orders, and the ways that inter-branch and inter-governmental disagreements about these orders were resolved.
Contributions of fundamental frequency and timbre to vocal emotion perception and their electrophysiological correlates
Authors: Nussbaum Christine , Schirmer Annett , Schweinberger Stefan R 
Published: May 2022

Abstract
Our ability to infer a speaker’s emotional state depends on the processing of acoustic parameters such as fundamental frequency (F0) and timbre. Yet, how these parameters are processed and integrated to inform emotion perception remains largely unknown. Here we pursued this issue using a novel parameter-specific voice morphing technique to create stimuli with emotion modulations in only F0 or only timbre. We used these stimuli together with fully modulated vocal stimuli in an event-related potential (ERP) study in which participants listened to and identified stimulus emotion. ERPs (P200 and N400) and behavioral data converged in showing that both F0 and timbre support emotion processing but do so differently for different emotions: Whereas F0 was most relevant for responses to happy, fearful and sad voices, timbre was most relevant for responses to voices expressing pleasure. Together, these findings offer original insights into the relative significance of different acoustic parameters for early neuronal representations of speaker emotion and show that such representations are predictive of subsequent evaluative judgments.
Sleep disturbances, circadian activity, and nocturnal light exposure characterize high risk for and current depression in adolescence
Authors: Tonon André Comiran , Constantino Débora Barroggi , Amando Guilherme Rodriguez , Abreu Ana Carolina , Francisco Ana Paula , de Oliveira Melissa Alves Braga , Pilz Luísa K , Xavier Nicóli Bertuol , Rohrsetzer Fernanda , Souza Laila , Piccin Jader , Caye Arthur , Petresco Sandra , Manfro Pedro H , Pereira Rivka , Martini Thaís , Kohrt Brandon A , Fisher Helen L , Mondelli Valeria , Kieling Christian , Hidalgo Maria Paz Loayza 
Published: May 2022

Abstract

Study Objectives
Major depressive disorder (MDD) in adolescence is associated with irregularities in circadian rhythms and sleep. The characterization of such impairment may be critical to design effective interventions to prevent development of depression among adolescents. This study aimed to examine self-reported and actimetry-based circadian rhythms and sleep–wake behavior associated with current MDD and high risk (HR) for MDD among adolescents.

Methods
Ninety-six adolescents who took part in the IDEA-RiSCo study were recruited using an empirically developed depression-risk stratification method: 26 classified as low risk (LR), 31 as HR, and 39 as a current depressive episode (MDD). We collected self-report data on insomnia, chronotype, sleep schedule, sleep hygiene as well as objective data on sleep, rest-activity, and light exposure rhythms using actimetry for 10 days.

Results
Adolescents with MDD exhibited more severe insomnia, shorter sleep duration, higher social jetlag (SJL), lower relative amplitude (RA) of activity, and higher exposure to artificial light at night (ALAN) compared with the other groups. They also presented poorer sleep hygiene compared with the LR group. The HR group also showed higher insomnia, lower RA, higher exposure to ALAN, and higher SJL compared with the LR group.

Conclusions
HR adolescents shared sleep and rhythm alterations with the MDD group, which may constitute early signs of depression, suggesting that preventive strategies targeting sleep should be examined in future studies. Furthermore, we highlight that actimetry-based parameters of motor activity (particularly RA) and light exposure are promising constructs to be explored as tools for assessment of depression in adolescence.
Pretreatment with Perlecan-Conjugated Laminin-E8 Fragment Enhances Maturation of Grafted Dopaminergic Progenitors in Parkinson’s Disease Model
Authors: Adachi Hiromasa , Morizane Asuka , Torikoshi Sadaharu , Raudzus Fabian , Taniguchi Yukimasa , Miyamoto Susumu , Sekiguchi Kiyotoshi , Takahashi Jun 
Published: May 2022

Abstract
The therapeutic effect of a cell replacement therapy for Parkinson’s disease (PD) depends on the proper maturation of grafted dopaminergic (DA) neurons and their functional innervation in the host brain. In the brain, laminin, an extracellular matrix protein, regulates signaling pathways for the survival and development of neurons by interacting with integrins. The heparan sulfate (HS) chain binds mildly to various neurotrophic factors and regulates their intracellular signaling. Perlecan-conjugated laminin 511/521-E8 fragments (p511/p521) were designed to contain an integrin-binding site and HS chains. Here we examined the effect of treating DA progenitors with p511/p521 prior to transplantation in rodent PD models. In vitro and in vivo experiments showed that p511/p521 treatment enhanced the maturation and neurite extension of the grafted DA progenitors by activating RAS-ERK1/2 signaling. This strategy will contribute to an efficient cell replacement therapy for PD in the future.
Aldicarb-related suicide attempt cases in North of France (2012–2021)
Authors: Lenski Marie , Letrillart Agnès , Gish Alexandr , Nisse Patrick , Gaulier Jean-michel , Allorge Delphine 
Published: May 2022

AbstractAldicarb, a carbamate pesticide banned in France since 2008, represents a persistent risk of human poisoning. There is no up-to-date picture of aldicarb intentional poisonings associated to detailed diagnosis and clinical management of cases to evaluate the effect of its ban on intoxication risk reduction, including suicide. This retrospective epidemiological study describes cases of suicidal intoxication from the Hauts-de-France region between 2012 and 2021 and illustrates this situation through one analytically documented case. 60 cases were collected, mostly presenting a pathognomonic symptomatology. Thirty-five victims presented a muscarinic syndrome (58.3%), 14 a nicotinic syndrome (23.3%), and 37 a central nervous system impairment (61.7%). Hospitalization was necessary for 44 cases (73.3%), with 2 fatal evolutions. Diagnosis was based on the blood cholinesterase activities. Among the 25 cases with toxicology results, 45.8% presented a moderate decrease of acetylcholinesterase activity, whereas 87.5% presented a strong decrease of butyrylcholinesterase activity. Blood or urine detection of aldicarb and its metabolites may be considered in therapeutic management, although their quantification is unlikely to change the emergency medical care. Our study updates epidemiology of aldicarb poisoning at a regional level, highlighting a persisting health threatening situation with banned pesticides. More than 65% of national cases occurred in this agricultural area, aldicarb remaining available from storage of previously purchased products. Robust evidence is presented that acute poisoning is an ongoing major global public health challenge. There is a need for continued international efforts in risk reduction, knowledge, and information strategies.
Pelvic Osteotomy in Patients With Previous Sacral-Alar-Iliac (SAI) Fixation
Authors: Mun Frederick , Vankara Ashish , Suresh Krishna V. , Margalit Adam , Crasta Nikitha , Sponseller Paul D. 
Published: May 2022

Introduction:
Patients with neuromuscular disease are at high risk for developing hip dysplasia and scoliosis. The purpose of this study was to investigate the technical challenges and outcomes of pelvic osteotomy in patients with prior sacral-alar-iliac (SAI) fixation.

Methods:
We reviewed clinical and radiographic records of patients aged 18 years and below who underwent pelvic osteotomy after SAI fixation. We recorded technical challenges during the osteotomy, time from SAI fixation to osteotomy, type of osteotomy, migration index, and distance from the SAI screw to the acetabulum. A 2-sample Wilcoxon rank-sum test was used to assess the data.

Results:
Nineteen patients were included. Technical challenges were defined as having greater intraoperative fluoroscopy times and noted difficult osteotomy in the operative report. The mean time from SAI fixation to pelvic osteotomy was 2.2±1.5 years. For all 12 Chiari osteotomies, the ilium could not be laterally displaced; however, medial displacement of the distal segment of the osteotomy allowed adequate coverage. All 7 Dega osteotomies were performed by cutting the cortex at the tip of the SAI screw. The screw improved proximal leverage and provided a strong buttress for bone graft. The mean migration index before pelvic osteotomy was 59±19%, and at most recent follow-up was 13±4%. Twelve patients, who had a noted complicated osteotomy, had SAI screws that were ≤1.87 cm (P<0.01) from the acetabulum and significantly increased intraoperative fluoroscopy time (1.76 vs. 1.18 min, P<0.01).

Conclusions:
The presence of SAI screws may cause iliac osteotomies to be technically challenging if the tip of the SAI screw is ≤1.87 cm to the acetabulum. When initially implanting SAI screws in neuromuscular patients, surgeons should attempt to place screw tips ∼2 cm from the acetabulum in the event these patients require subsequent pelvic osteotomy.

Level of Evidence:
Level IV.
Representation of Global Health Initiatives in Plastic Surgery Training
Authors: Naides Alexandra I. , Kapadia Kailash , Salem Jena , Dalena Margaret , Cohen David , Ayyala Haripriya S. , Lee Edward S. 
Published: May 2022

Introduction
Over the last several years, various social media platforms have been used to increase collaboration, education, and research internationally. The purpose of this study was to evaluate how plastic surgery residency programs use social media to promote global surgery education, research, and collaboration.

Methods
A full list of active integrated residency programs was obtained from the American College of Academic Plastic Surgeons website. A total of 82 programs were identified. Instagram, Facebook, and Twitter were searched for active accounts. Only accounts dedicated to plastic surgery programs or had a significant amount of residency-related content were included. Inclusion criteria included posts relating to global health, global surgery, mission trips, international plastic surgery education, and global surgery research. Caption language was analyzed and classified as either “resident education in global surgery,” “an individual’s interest in global surgery,” “research in global surgery,” “visiting international professor,” or “global collaboration.” Image content was then assessed for “images with patients,” “images with other surgeons/residents,” “images of international location,” a combination thereof, “other,” or “none.”

Results
Instagram was by far the most used by plastic surgery residency programs with a total of 76 programs having accounts compared with only 32 programs and 33 programs having Facebook and Twitter accounts, respectively. There was no significant difference in the proportional number of posts relating to global surgery across the 3 platforms (P = 0.1911). On all 3 social media platforms, the majority of posts fell into the category of “international collaboration” with 42.5%, 50.7%, and 48.8% on Instagram, Facebook, and Twitter, respectively. None of the caption categories examined showed a significantly different proportion of posts across the 3 platforms. Image analysis showed a significant difference in the proportional representation across the 3 platforms in the categories of “images with other surgeons/residents” (P = 0.0196) “images with patients” (P = 0.0082), combination (P = 0.0225), and other (P = 0.0114).

Conclusions
Although social media offers programs an easy way to promote global surgery research, collaboration, and education with a wider audience, it is being underused for this purpose as evident by the relatively lower number of postings on each platform relating to this content.