bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of metals in Pork Lard: A rapid and low cost sample preparation by RP-DLLME
Authors: Santos Mayara Patricia Oliveira , Carboni Nicoli Andressa , Flores Eder Lisandro Moraes , Canan Cristiane , Leite Oldair Donizeti , Drunker Deisy Alessandra 
Published: August 2022

Abstract
ABSTRAT
In this work, a new method was proposed for determination of Cu, Fe, Mg, Mn and Ni in pork lard by flame atomic absorption spectrometry (F AAS) was developed using reverse phase dispersive liquid-liquid microextraction (RP-DLLME) for sample preparation. The parameters such as type, volume and concentration of dispersing (n-propanol and isopropanol) and extracting (HNO3 and HNO3 + HCl (1:1 v/v) solvents, ultrasound and centrifugation time were evaluated. For the determination of analytes by F AAS, external using aqueous reference solutions was performed for the metals evaluated in the range from 0.25 to 1.5 mg L-1. The limits of quantification for Cu, Fe, Mg, Mn and Ni were 0.0106; 0.0136; 0.0042; 0.0169 and 0.0124 µg Kg-1, respectively. The accuracy was estimated to be between 95.64 to 104.42% and precision (RSD) less than 1% for all analytes. The method was applied to different brands of pork lard (n = 5), where the recovery was between 90.2 and 103.2% and RSD between 0.024 and 0.094. After that, a risk characterization was performed, in which it was obtained for Fe 0.43 to 2.79% and for Mg 0.21 to 4.22% in relation to the recommended daily intake and for Ni 5.13 to 25.83% to the tolerable upper intake level. The risk quotient was less than 1 x 105 so that even sensitive populations are unlikely to suffer adverse effects.
Effects of different fractions of inspired oxygen on gas embolization during hysteroscopic surgery: A double-blind randomized controlled trial
Authors: Deng Xi , Zhou Jieshu , Diao Min , Li Hao , Lin Xuemei 
Published: August 2022

Abstract
BackgroundGas embolism is a common complication of hysteroscopic surgery, which causes serious concern among gynecologists and anesthesiologists due to the potential risk to the patients. The influencing factors of gas embolism in hysteroscopic surgery have been extensively studied. However, the effect of the oxygen concentration inhaled by patients on gas embolism during hysteroscopic surgery remains elusive. So, we ﻿designed a double-blind randomized controlled trial to determine whether different inhaled oxygen concentrations influence the occurrence of gas embolism during hysteroscopic surgery.MethodsThis prospective, double-blind, randomized controlled trial enrolled 162 adult patients aged between 20 and 65 years undergoing elective hysteroscopic surgery who were randomly divided into three groups with fractions of inspired oxygen of 30%, 50% and 100%. Transthoracic echocardiography (4-chamber view) was used to evaluate whether the gas embolism occurred. Before the start of surgery, 4-chamber view was continuously monitored.ResultsThe number of gas embolism in group A, B and C was 36 (69.2%), 30 (55.6%) and 24 (44.4%) respectively. The incidence of gas embolism gradually decreased with the increase of inhaled oxygen concentration(P=0.031).ConclusionsIn hysteroscopic surgery, a higher oxygen concentration inhaled by patients could reduce the incidence of gas embolism, indicating that a higher inhaled oxygen concentration, especially 100%, could be recommended for patients during hysteroscopic surgery.Trial registrationChinese Clinical Trial Registry(23/05/2020, ChiCTR2000033202)
A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression
Authors: NEGRI RODOLFO , Nisio Elena Di , Licursi Valerio , Mannironi Cecilia , Buglioni Valentina , Paiardini Alessandro , Robusti Giulia , Noberini Roberta , Bonaldi Tiziana 
Published: August 2022

Abstract
KDM5B histone demethylase is overexpressed in many cancers and plays an ambivalent role in oncogenesis, depending on the specific context. This ambivalence could be explained by the expression of KDM5B protein isoforms with diverse functional roles, which could be present at different levels in various cancer cell lines. We show here that one of these isoforms, namely KDM5B-NTT, accumulates in breast cancer cell lines up to 50-60% of the total due to remarkable protein stability relative to the canonical PLU-1 isoform, which shows a much faster turnover. This isoform is the truncated and catalytically inactive product of an mRNA with a transcription start site downstream of the PLU-1 isoform, and the consequent usage of an alternative ATG for translation initiation. It also differs from the PLU-1 transcript in the inclusion of an additional exon (exon-6), previously attributed to other putative isoforms. Overexpression of this isoform in MCF7 cells leads to an increase in bulk H3K4 methylation and induces derepression of a gene cluster, including the tumor suppressor Cav1 and several genes involved in the interferon-alpha and -gamma response. We discuss the relevance of this finding considering the hypothesis that KDM5B may possess regulatory roles independent of its catalytic activity.
Audience Effects in Sooty Mangabey Agonistic Behaviour
Authors: Quintero Fredy , Touitou Sonia , Magris Martina , Zuberbühler Klaus 
Published: August 2022

Abstract
Aggression is primarily a proactive mechanism for animals engaged in competitive interactions, in order to repel predators, access resources or pursue other self-serving interests. A secondary function is of a reactive, punitive nature where it is used as a partner control mechanism to impose cooperation and tolerance. Both functions are available to higher-ranking individuals directly interacting with lower-ranking opponents, which raises the question of whether and how lower-ranking individuals use aggression indirectly to secure tolerance from others. Here, we investigated how free-ranging sooty mangabeys, a socially tolerant forest dwelling primate, used aggression during competitive interactions inside food patches in front of uninvolved bystanders. As predicted, subjects used aggression to displace lower-ranking competitors from food but we also found that they generally refrained from severe aggression in front of friends and small audiences. Mild aggression, in contrast, was significantly augmented in front of high-ranking audiences, often accompanied by vocalisations. We discuss these patterns of audience-dependent aggressive behaviour and concluded that, while mangabeys generally seem to pursue selfish foraging goals, they are capable of adjusting levels of aggression when observed by socially relevant audiences.
How to Precisely Detect and Assess the Street Trees Risk ？ Risk Assessment Based on Precise Diagnosis Technology and Its Application
Authors: He Kun , Wei Longlong , Li Nan , Wang Junjie , Ye Sihao , Wang Benyao 
Published: August 2022

Abstract
Traditional visual tree assessment is suitable for the rapid, large-scale assessment of urban tree risks, but it cannot precisely determine internal decay in trunks and underground root systems, may leading to highly subjective evaluation results. A risk matrix-based street tree risk assessment system was established by combining VTA method, nondestructive detection techniques, information acquisition and data analysis using GIS. The method was used to conduct risk detection and assessment on 1,001 street trees with a diameter at breast height greater than 40 cm in a Historic Features Protection Area of Shanghai. The result showed that: 1) The branch and the trunk risk possibility of the majority of street trees were at level 2 or below, while the root risk possibility of more than1/3 of the trees was at level 3 or above. 2) The risk level of 23% street trees was moderate or above, while that of other trees was at an acceptable level. 3) The street tree risk level shows a strong correlation with the presence of tree cavities, diseases and insect pests, the depth and range of root distribution, leaning, and internal decay in trunks, and the risk points are concentrated in the trunk and root system. Risk assessment of street trees based on precision diagnosis techniques can provide a new assessment system for the trees risk assessment in important urban areas, and the approach is conducive to the refined management of urban trees.
Clinicopathological characteristics and molecular analysis of lung cancer associated with ciliated muconodular papillary tumor/bronchiolar adenoma
Authors: Higashiyama Masahiro , Motoi Noriko , Yotsukura Masaya , Yoshida Yukihiro , Nakagawa Kazuo , Yagishita Shigehiro , Shirasawa Masayuki , Yoshida Tatsuya , Shiraishi Kouya , Kohno Takashi , Ohe Yuichiro , Watanabe Shun-ichi 
Published: August 2022

Abstract
Ciliated muconodular papillary tumor/bronchiolar adenoma (CMPT/BA) is a benign tumor with a high frequency of BRAF or EGFR mutations. It remains unclear whether CMPT/BA is associated with a specific type of lung cancer. Thus, we studied the clinicopathological and genetic characteristics of cases with coexisting CMPT/BA and primary lung cancer to explore this relationship. Among 1945 patients with resected stage 0-III primary lung cancer between 2015–2018, we identified eight patients with concurrent CMPT/BA (lung cancer associated with CMPT/BA; LCCM). We compared the gene mutation status of each lesion according to the target sequence using macro-dissected formalin-fixed paraffin-embedded specimens. Whole-exon sequencing (WES) was performed for six lung cancer cases. The LCCM cohort was male-dominant (male:female = 6:2); the median age was 72 years (range 66–81); and most were smokers (six smokers). The most common histological type corresponding to LCCM was adenocarcinoma; however, two squamous cell carcinomas and one small cell carcinoma were also detected. CMPT/BA lesions in LCCM showed a median size of 0.5 cm (range 0.3–1.1). The mutational test revealed no shared mutations between CMPT/BA and lung cancer, except for one invasive mucinous adenocarcinoma (IMA) harboring an HRAS mutation (I46N, c.137T > A). HRAS (I46N) is a rare variant, and all tumors showed an approximately 50% variant allele frequency, suggesting a single nucleotide polymorphism. Other driver gene alterations in lung cancer included EGFR (InDel, n = 2), BRAF (V600E) (n = 1), KRAS (n = 2), GNAS (n = 1), and TP53 (n = 2) mutations. BRAF (V600E) was the most frequent mutation in CMPT/BA. In contrast, lung cancer showed no specific trend in driver gene mutations. In conclusion, our study revealed differences in the gene mutation profiles of CMPT/BA and lung cancer in coexisting cases, suggesting a mostly independent tumorigenesis of CMPT/BA from lung cancer, except for one IMA with a rare HRAS SNV.
Comprehensive Analysis of Clinicopathologic Features and p53 Mutation in Neuroendocrine Neoplasms of the Breast: Experience from a Large Academic Center
Authors: Shafi Saba , Hu Yan , Parwani Anil V. , Ding Qingqing , Li Zaibo 
Published: August 2022

Abstract
Purpose The recent WHO classification of breast cancer (2019) categorizes breast carcinoma with neuroendocrine (NE) differentiation into three morphologically distinct subtypes: well-differentiated neuroendocrine tumor (NET), poorly differentiated neuroendocrine carcinoma (NEC), and invasive breast carcinoma, no special type with neuroendocrine differentiation (IBC-NST-NE). Data regarding the prognostic significance of neuroendocrine differentiation are conflicting and an association, if any, between p53 mutation and neuroendocrine differentiation is largely unknown. Methods We examined p53 expression and other clinicopathologic characteristics in three types of invasive breast carcinoma with NE differentiation in a cohort of sixty-three patients, including 45 IBC-NST with NE differentiation, 10 NETs and 8 NECs. Results No significant difference of clinicopathologic feature was observed between IBC-NST with NE differentiation and NET, but NECs showed significantly lower expressions of hormone receptors, more mutated p53 and higher frequency of distant metastases than IBC-NST with NE differentiation and NETs.Conclusions NECs of the breast are genetically and clinically different from IBC-NST-NEs and NETs of the breast.
Dexamethasone combined with hydromorphone preemptive analgesia alleviates pain and complications after jaw cyst enucleation: a randomized controlled trial
Authors: Zhou Wang , Liu Fan , Fang Junbiao , Han Lianghui 
Published: August 2022

Abstract
Background
Dexamethasone is widely used in the prevention of postoperative complications in oral surgery and strengthening the analgesic effect after anesthesia, but the efficacy is controversial, and the relationship between postoperative complications and pain is still unclear. The purpose of this study was to evaluate the analgesic effect of dexamethasone in the treatment of jaw cyst and to explore the relationship between postoperative complications and pain.
Methods
We conducted a prospective, randomized, double-blind clinical trial. 120 patients were divided into two groups, dexamethasone group ( group D) and control group (Group C). All patients were given 0.02mg/kg of hydromorphone to relieve pain in advance at 10 minutes before the beginning of operation. Meanwhile, dexamethasone was injected 0.2mg/kg intravenously in group D and normal saline was injected in group C. The primary endpoint was pain intensity at 2h, 6h, 12h, 24h and 48h after surgery. The secondary endpoints were the incidence and extent of complications after surgery, including facial swelling and trismus.
Results
Compared with group C, the VAS scores at rest at 2, 6, 12 and 24 h after surgery and during mobilization at 2h, 6h, 12h, 24h and 48h after surgery in group D were significantly lower (P < 0.05). The degree of facial swelling and trismus in group D at 12, 24 and 48 h after surgery was significantly lower than that in group C (P < 0.05), but there was no difference at 6 hours after operation (P > 0.05). The C-reactive protein (CRP) level at 24 h after operation in group D was lower than group C (P < 0.05), but there was no significant difference in blood glucose concentration between the two groups (P > 0.05).
Conclusion
Dexamethasone can reduce the degree of facial swelling and trismus after jaw cyst surgery by inhibiting the production of inflammation, which alleviated the postoperative pain of patients significantly. In addition, it did not increase the risk of hyperglycemia.
Trial registration:
This study was registered with the Chinese Clinical Trial Registry on May 07, 2020 (URL: http://www.chictr.org.cn/showproj.aspx?proj=53344. Registry number: ChiCTR2000032693 ). Registered on 07/05/2020.
Human skin specific long noncoding RNA HOXC13-AS regulates epidermal differentiation by interfering with Golgi-ER retrograde transport
Authors: Zhang Letian , Piipponen Minna , Liu Zhuang , Li Dongqing , Bian Xiaowei , Toma Maria , Sommar Pehr , Landén Ning Xu 
Published: August 2022

Abstract
After a skin injury, keratinocytes switch from a state of homeostasis to one of regeneration leading to the reconstruction of the epidermal barrier. The regulatory mechanism of gene expression underpinning this key switch during human skin wound healing is enigmatic. Long noncoding RNAs (lncRNAs) constitute a new horizon in the understanding of the regulatory programmes encoded in the mammalian genome. By comparing the transcriptome of an acute human wound and skin from the same donor as well as keratinocytes isolated from these paired tissue samples, we generated a list of lncRNAs showing changed expression in keratinocytes during wound repair. Our study focused on HOXC13-AS, a recently evolved human lncRNA specifically expressed in epidermal keratinocytes, and we found that its expression was temporally downregulated during wound healing. In line with its enrichment in suprabasal keratinocytes, HOXC13-AS was found to be increasingly expressed during keratinocyte differentiation, but its expression was reduced by EGFR signalling. After HOXC13-AS knockdown or overexpression in human primary keratinocytes undergoing differentiation induced by cell suspension or calcium treatment and in organotypic epidermis, we found that keratinocyte differentiation was promoted, while the cell inflammatory response was suppressed. Moreover, RNA pull-down assays followed by mass spectrometry and RNA immunoprecipitation analysis revealed that mechanistically HOXC13-AS sequestered the coat complex subunit alpha (COPA) protein and interfered with Golgi-to-endoplasmic reticulum (ER) molecular transport, resulting in ER stress and enhanced keratinocyte differentiation. In summary, we identified HOXC13-AS as a crucial regulator of human epidermal differentiation.
Superoxide dismutase as a protective factor for early renal damage in hypertensive patients
Authors: Yu Xiaoqian , Rui Xu , Fei Zheng , Lin Lin , Wen Huang , Xia Wu 
Published: August 2022

Abstract
Background: Oxidative stress had been linked to hypertensive renal impairment in previous investigations. Superoxide dismutase (SOD) was a clinically available oxidative stress biomarker. The association between SOD and the early renal damage (ERD) of hypertension has not been established. Methods: A total of 690 patients with essential hypertension (EH) were retrospectively collected in Shandong Provincial Qianfoshan Hospital from January 2017 to December 2018. Patients were divided into hypertension with ERD group and hypertension without early renal damage (NRD) group. Clinical data from patients were collected and compared between the two groups. Spearman correlation analysis was used to analyze the correlation between UACR and SOD. Univariate and multivariate logistic regression analyses were used to screen for the risk factors for ERD. Results: Our research included 556 cases in the NRD group and 134 cases in the ERD group. The average age of the study participants was 60.24±11.74 years, and the mean values of SBP and DBP were 145.13 mmHg (1 mmHg = 0.133 kPa) and 85.97 mmHg. Spearman correlation analysis showed that a negative correlation between SOD and UACR (P ＜ 0.001). Multivariate logistic regression analysis showed that SOD was an independent protective factor for hypertension with ERD. Conclusion: SOD was an independent protective factor for early renal damage in hypertensive patients, providing a new direction for the treatment of early renal damage in hypertensive patients.
Microlesion effect in the associative sub-region of the subthalamic nucleus is related to worsened sleep outcomes
Authors: Zhang Jianguo , Ma Ruoyu , Yin Zixiao , Chen Yingchuan , Yuan Tianshuo , An Qi , Gan Yifei , Xu Yichen , Jiang Yin , Du Tingting , Yang Anchao , Meng Fangang , Zhu Guanyu 
Published: August 2022

Abstract
Objective
To examine the influence of the microlesion effect (MLE) after subthalamic nucleus (STN) deep brain stimulation (DBS) on sleep quality in Parkinson’s disease (PD) patients and the correlates of sleep changes.
Methods
In 78 PD patients who underwent bilateral STN-DBS surgery, we assessed and compared the sleep qualities, motor performances, anti-parkinsonian drug dosage, emotional conditions at preoperative baseline and before implantable pulse generator activation (i.e., 1-month postoperatively). We determined the possible related factors of sleep outcomes using Spearman correlations and visualized the electrodes via Lead-DBS toolbox, simulated and extracted the MLE-engendered volume of tissue lesioned (VTL) based on uniform settings, and conducted sweet-spot analysis within the STN.
Results
Significant improvements induced by MLE were observed in sleep (P < 0.001), motor performances (P < 0.001), and emotions (P < 0.001). Motor and emotional improvements were both positively correlated with sleep changes. The VTL in STN associative sub-regions was positively correlated with sleep deterioration (r = 0.348, P = 0.002), and that only the left STN showed statistical significance (r = 0.327, P = 0.004), which was further corroborated by the sweet-spot analysis. Stepwise regression analysis showed that the VTL in associative sub-regions was an independent factor that negatively influenced sleep outcomes.
Conclusion
The MLE of STN-DBS can overall improve sleep quality in PD patients, with the change correlating with motor and emotional improvement. However, independent of these two factors and other demographics, the MLE in the associative sub-region of the STN, left particularly, may result in worsened sleep outcomes.
Protective effect of NeuroEPO in the reproduction of diabetic rats.
Authors: Fernández-Romero Tammy , Clapés-Hernández Sonia , Pérez Carlos L , Núñez-López Nínive , Suárez-Román Gipsis , Fernández Gisselle 
Published: August 2022

Abstract
Introduction: pregestational diabetes constitutes a reproductive risk, which requires new treatment strategies. Considering that NeuroEPO, a variant of the recombinant human erythropoietin produced in Cuba, has neuroprotective and hypoglycemic effects, the aim was to evaluate the protective effect of NeuroEPO on the reproduction of diabetic rats.Material and Methods: four groups of adult female Wistar rats with streptozotocin-induced diabetes were used. During pregnancy, one group received the vehicle and the rest different doses of NeuroEPO (0.5 mg/kg, 0.75 mg/kg, and 1 mg/kg) subcutaneously, on alternate days, for six applications. A group of non-diabetic rats was used as a control. Glycemia and reproductive variables were evaluated. For comparisons, Analysis of Variance and Fisher's Exact Test was used. The differences were considered significant with p-values less than 0.05.Results: the group with vehicle presented maintained hyperglycemia, fewer implantations, and embryos, and increased gestational losses. In the group receiving 0.5 mg/kg of NeuroEPO, glycemia decreased significantly and the results of the reproductive variables were similar to the group of non-diabetic rats. With higher doses of NeuroEPO, gestational losses were increased. No congenital malformations were identified in either group.Conclusions: the repeated administration of 0.5 mg/kg of NeuroEPO has a beneficial effect on the reproduction of diabetic rats, which may be associated with the reduction of hyperglycemia. Other cytoprotective mechanisms of NeuroEPO should be evaluated in future studies.
Sexual Dimorphism of the Fetal Brain Biometry: An MRI Based Study
Authors: Gafner Michal , Sade Eliel Kedar , Barzilay Eran , Katorza Eldad 
Published: August 2022

Abstract
Purpose: Fetal growth assessment is a key component of prenatal care. Sex-specific fetal brain nomograms on ultrasound are available and are clinically used. In recent years, the use of fetal MRI has been increasing, however, there are no sex-specific fetal CNS nomograms on MRI. Study aims were to asses the differences in fetal brain biometry and growth trajectories, and to create population-based standarts of the fetal brain on MRI.Methods: In this cross-sectional study, brain structures of singelton fetuses with normal brain MRI scans were analyzed: biparietal diameter, occipitofrontal diameter, trans-cerebellar diameter and the corpus callosum were measured and and converted into centiles. Sex-specfic nomograms were created.Results: A total of 3,848 MRI scans were performed in one tertiary medical center between 2011-2019, of them 598 fetuses met the inclusion criteria; 300 males and 298 females between 28 to 37 weeks gestation. Males had significantly larger occipitofrontal diameter than females (median centile 75%, IQR 54-88%; median 61%, IQR 40-77%) and biparietal diameter (median 63%, IQR 42-82%; median 50%, IQR 25-73%) respectively (p<0.001). The cerebellum had the greatest growth rate, with 1.5-fold increase in diameter between 28 and 37-weeks’ gestation, and no measurements difference between the sexes (p=0.239). No significant difference was found in the corpus callosum (p=0.074).Conclusion: Measuring both sexes on the same nomograms may result in over-estimation of male fetuses and under-estimation of females. We provide fetal sex-specific nomograms on two-dimensional MRI.