bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic yield of liquid‐based cytology in serial pancreatic juice aspiration cytological examination
Authors: Kitagawa Koh , Mitoro Akira , Tomooka Fumimasa , Asada Shohei , Fujinaga Yukihisa , Nishimura Norihisa , Kaji Kosuke , Kawaratani Hideto , Akahane Takemi , Ozutsumi Takahiro , Kaneko Miki , Fujimoto Yuki , Tsuji Yuki , Enomoto Masahide , Takeda Soichi , Murata Koji , Kubo Takahiro , Iwai Satoshi , Koizumi Aritoshi , Shibamoto Akihiko , Suzuki Junya , Tanaka Misako , Matsuda Takuya , Yorioka Nobuyuki , Masuda Hiroyuki , Takami Masayoshi , Yoshiji Hitoshi 
Published: October 2022

AbstractObjectivesSerial pancreatic juice aspiration cytological examination (SPACE) via endoscopic retrograde cholangiopancreatography is a useful diagnostic method for early‐stage pancreatic cancer, such as carcinoma in situ that are difficult to diagnose by endoscopic ultrasound‐guided fine needle aspiration (EUS‐FNA). However, the diagnostic accuracy of SPACE is low, which is attributed to problems regarding specimen treatment. Hence, we evaluated the diagnostic efficacy of liquid‐based cytology (LBC) in pancreatic juice cytology for pancreatic cancer.MethodsWe retrospectively analyzed 24 patients with suspected pancreatic cancer that was difficult to diagnose by endoscopic ultrasound‐guided fine needle aspiration who underwent SPACE using LBC between April 2017 and April 2021.ResultsThe most common reason for performing SPACE was localized stenosis of the main pancreatic duct without a mass. Eleven patients were diagnosed with malignancy after surgical resection, nine of whom had pancreatic ductal adenocarcinoma. Ten patients were diagnosed as benign after a follow‐up of more than 1 year. The nine cases of malignancy were diagnosed before surgical resection by SPACE using LBC, with a sensitivity of 81.8% and specificity of 100%. The overall diagnostic accuracy was 91.7%. A total of 152 LBC examinations were performed via SPACE, with an adequate sample collection rate of 88.9%. No adverse events, including acute pancreatitis, occurred after endoscopic retrograde cholangiopancreatography.ConclusionSPACE with LBC offers good diagnostic efficacy in patients with pancreatic cancer that is difficult to diagnose by endoscopic ultrasound‐guided fine needle aspiration.
Case Study in 21st-Century Ecotoxicology: Using In Vitro Aromatase Inhibition Data to Predict Reproductive Outcomes in Fish In Vivo
Authors: Villeneuve Daniel L. , Blackwell Brett R. , Blanksma Chad A. , Cavallin Jenna E. , Cheng Wan-Yun , Conolly Rory B. , Conrow Kendra , Feifarek David J. , Heinis Larry J. , Jensen Kathleen M. , Kahl Michael D. , Milsk Rebecca Y. , Poole Shane T. , Randolph Eric C. , Saari Travis W. , Watanabe Karen H. , Ankley Gerald T. 
Published: October 2022

Abstract
To reduce the use of intact animals for chemical safety testing, while ensuring protection of ecosystems and human health, there is a demand for new approach methodologies (NAMs) that provide relevant scientific information at a quality equivalent to or better than traditional approaches. The present case study examined whether bioactivity and associated potency measured in an in vitro screening assay for aromatase inhibition could be used together with an adverse outcome pathway (AOP) and mechanistically based computational models to predict previously uncharacterized in vivo effects. Model simulations were used to inform designs of 60-h and 10–21-day in vivo exposures of adult fathead minnows (Pimephales promelas) to three or four test concentrations of the in vitro aromatase inhibitor imazalil ranging from 0.12 to 260 µg/L water. Consistent with an AOP linking aromatase inhibition to reproductive impairment in fish, exposure to the fungicide resulted in significant reductions in ex vivo production of 17β-estradiol (E2) by ovary tissue (≥165 µg imazalil/L), plasma E2 concentrations (≥74 µg imazalil/L), vitellogenin (Vtg) messenger RNA expression (≥165 µg imazalil/L), Vtg plasma concentrations (≥74 µg imazalil/L), uptake of Vtg into oocytes (≥260 µg imazalil/L), and overall reproductive output in terms of cumulative fecundity, number of spawning events, and eggs per spawning event (≥24 µg imazalil/L). Despite many potential sources of uncertainty in potency and efficacy estimates based on model simulations, observed magnitudes of apical effects were quite consistent with model predictions, and in vivo potency was within an order of magnitude of that predicted based on in vitro relative potency. Overall, our study suggests that NAMs and AOP-based approaches can support meaningful reduction and refinement of animal testing. Environ Toxicol Chem 2023;42:100–116. © 2022 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Abstract
Key events in the adverse outcome pathway linking aromatase inhibition to reduced cumulative fecundity and spawning that were impacted following 60 h, 10 days, or 21 days of waterborne exposure to imazalil. Black shading indicates that no measurements were made. X indicates that activity was confirmed in vitro. Checkmark indicates that the measurement was made, with color bar indicating the lowest-observed-effect concentration.
A Multiplex Gene Expression Assay for Direct Measurement of RNA Transcripts in Crude Lysates of the Nematode Caenorhabditis elegans Used as a Bioanalytical Tool
Authors: Karengera Antoine , Bao Cong , Bovee Toine F. H. , Dinkla Inez J. T. , Murk Albertinka J. 
Published: October 2022

Abstract
Gene expression profiling in Caenorhabditis elegans has been demonstrated to be a potential bioanalytical tool to detect the toxic potency of environmental contaminants. The RNA transcripts of genes responding to toxic exposure can be used as biomarkers for detecting these toxins. For routine application in environmental quality monitoring, an easy-to-use multiplex assay is required to reliably quantify expression levels of these biomarkers. In the present study, a bead-based assay was developed to fingerprint gene expression in C. elegans by quantitating messenger RNAs (mRNAs) of multiple target genes directly from crude nematode lysates, circumventing RNA extraction and purification steps. The assay uses signal amplification rather than target amplification for direct measurement of toxin-induced RNA transcripts. Using a 50-gene panel, the expression changes of four candidate reference genes and 46 target mRNAs for various contaminants and wastewaters were successfully measured, and the expression profiles indicated the type of toxin present. Moreover, the multiplex assay response was in line with previous results obtained with more time-consuming reverse-transcription quantitative polymerase chain reaction and microarray analyses. In addition, the transcriptomic profiles of nematodes exposed to wastewater samples and extracts prepared from tissues of swimming crabs were evaluated. The profiles indicated the presence of organic pollutants. The present study illustrates the successful development of a multiplex fluorescent bead–based approach using nematode C. elegans crude lysates for gene expression profiling of target RNAs. This method can be used to routinely fingerprint the presence of toxic contaminants in environmental samples and to identify the most biologically active fraction of the contaminant mixture in a toxicity identification and evaluation approach. Environ Toxicol Chem 2023;42:130–142. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Co-control of the haze pollution emissions in China: Insight from supply chains
Authors: Yu Yang , Chi Yulin , Du Yiming 
Published: October 2022

Abstract
Because of rapid economic development and the increase in social demand, China has been suffering from serious air pollution, in particular, haze pollution. To mitigate haze from the source, it is essential to achieve co-control of three important haze precursors: volatile organic compounds (VOCs), sulfur dioxide (SO2), and nitrogen oxide (NOx). In this study, we used the environmentally extended input–output model, structural path analysis, and structural path decomposition method to investigate changes in consumption-based emissions of three major haze precursors (i.e., NOx, SO2, and VOCs) in China during 2007–2017. First, the results revealed that fixed capital formation was the most critical final demand to co-control the three precursors. Investment in construction was the most important behavior for co-control. Second, the most crucial common path driving the changes in emissions of the three precursors was “transportation and warehousing→household consumption” during 2007–2012, and “electricity, gas, and water supply→household consumption” during 2012–2017. Finally, direct emission intensity of transportation and warehousing, and electricity, gas, and water supplies were critical to co-control precursors. The results of this study provided a comprehensive understanding of changes in haze precursor emissions driven by demand. Therefore, China must strengthen the co-control of multiple pollutant emissions on both the production and consumption sides by adjusting supply chains. Integr Environ Assess Manag 2023;19:1048–1063. © 2022 SETAC
Bullying perpetration and social status in the peer group: A meta‐analysis
Authors: Wiertsema Maria , Vrijen Charlotte , van der Ploeg Rozemarijn , Sentse Miranda , Kretschmer Tina 
Published: October 2022

AbstractIntroductionAssociations between bullying perpetration and social status vary, not only between different facets of social status but also between bullying in primary versus secondary school. The main aim of the present study was to meta‐analyse existing evidence regarding the prospective associations between bullying perpetration and various facets of social status, that is, popularity, peer acceptance, peer rejection, and social preference.MethodsElectronic databases were searched to identify studies on bullying perpetration and later social status published up to January 17, 2022. Multilevel random effects models were performed using Metafor and differences in effect sizes as a function of substantive and methodological moderators were tested.ResultsIn total, 116 effects were included from 18 publications, reporting on 17 different samples and more than 15,000 participants (mean age bullying assessment = 11.57 years, on average 51% female participants). Most samples were from the United States (7) or Europe (7). Overall, bullies were more popular, but also more rejected and scored lower on social preference compared with non‐bullies. These associations remained when effects were adjusted for previous social status and other confounders. No link between bullying perpetration and acceptance was found. There was little evidence that effect sizes differed as a function of moderators.ConclusionsBullies become more popular over time, but also have a higher risk of being rejected and being less socially preferred. Bullying perpetrators are more popular but also more rejected by their peers. There is no evidence that these links differ depending on sex or age at which perpetrators bully, reporter or type of bullying.
PTH Treatment Increases Cortical Bone Mass More in Response to Compression than Tension in Mice
Authors: Rooney Amanda M. , McNeill Tyler J. , Ross F. Patrick , Bostrom Mathias P.G. , van der Meulen Marjolein C.H. 
Published: October 2022

ABSTRACT
Parathyroid hormone (PTH) is an anabolic osteoporosis treatment that increases bone mass and reduces fracture risk. Clinically, the effects of PTH are site-specific, increasing bone mass more at the spine than the hip and not increasing bone mass at the radius. Differences in local loading environment between the spine, hip, and radius may help explain the variation in efficacy, as PTH and mechanical loading have been shown to synergistically increase bone mass. We hypothesized that differences in loading mode might further explain these variations. Owing to the curvature of the mouse tibia, cyclic compression of the hindlimb causes bending at the tibial midshaft, placing the anterior surface under tension and the posterior surface under compression. We investigated the combination of PTH treatment and tibial loading in an osteoblast-specific estrogen receptor-alpha knockout mouse model of low bone mass (pOC-ERαKO) and their littermate controls (LCs) and analyzed bone morphology in the tensile, compressive, and neutral regions of the tibial midshaft. We also hypothesized that pretreating wild-type C57Bl/6J (WT) mice with PTH prior to mechanical loading would enhance the synergistic anabolic effects. Compression was more anabolic than tension, and PTH enhanced the effect of loading, particularly under compression. PTH pretreatment maintained the synergistic anabolic effect for longer durations than concurrent treatment and loading alone. Together these data provide insights into more effective physical therapy and exercise regimens for patients receiving PTH treatment. © 2022 American Society for Bone and Mineral Research (ASBMR).
Coping with loneliness through consumption
Authors: Shrum L. J. , Fumagalli Elena , Lowrey Tina M. 
Published: October 2022

AbstractLoneliness is a complex set of aversive feelings that arises when people perceive that their belongingness needs are not being met. Usually, these feelings of loneliness are temporary because people successfully cope with their loneliness by connecting with others. However, for some people, their attempts to cope with loneliness are unsuccessful, and their loneliness becomes chronic, which can have severe consequences for their mental and physical health. Understanding the causes and consequences of loneliness is critical for developing interventions to reduce loneliness, a need made more urgent by the dramatic rise in reported loneliness over the last few decades. In this review, we provide a synthesis of the research on how people cope with loneliness through consumption situations and the extent to which these coping strategies are successful. We also provide a discussion of how the marketplace has responded to the rapidly increasing levels of chronic loneliness worldwide. We conclude with an agenda for future research to answer both basic and applied research questions regarding the causes, consequences, and underlying processes of loneliness.
Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop
Authors: Paolini Lucia , Monguió‐Tortajada Marta , Costa Marta , Antenucci Fabio , Barilani Mario , Clos‐Sansalvador Marta , Andrade André Cronemberger , Driedonks Tom A. P. , Giancaterino Sara , Kronstadt Stephanie M. , Mizenko Rachel R. , Nawaz Muhammad , Osteikoetxea Xabier , Pereira Carla , Shrivastava Surya , Boysen Anders Toftegaard , van de Wakker Simonides Immanuel , van Herwijnen Martijn J. C. , Wang Xiaoqin , Watson Dionysios C. , Gimona Mario , Kaparakis‐Liaskos Maria , Konstantinov Konstantin , Lim Sai Kiang , Meisner‐Kober Nicole , Stork Michiel , Nejsum Peter , Radeghieri Annalisa , Rohde Eva , Touzet Nicolas , Wauben Marca H. M. , Witwer Kenneth W. , Bongiovanni Antonella , Bergese Paolo 
Published: October 2022

AbstractExtracellular vesicles (EVs) large‐scale production is a crucial point for the translation of EVs from discovery to application of EV‐based products. In October 2021, the International Society for Extracellular Vesicles (ISEV), along with support by the FET‐OPEN projects, “The Extracellular Vesicle Foundry” (evFOUNDRY) and “Extracellular vesicles from a natural source for tailor‐made nanomaterials” (VES4US), organized a workshop entitled “massivEVs” to discuss the potential challenges for translation of EV‐based products. This report gives an overview of the topics discussed during “massivEVs”, the most important points raised, and the points of consensus reached after discussion among academia and industry representatives. Overall, the review of the existing EV manufacturing, upscaling challenges and directions for their resolution highlighted in the workshop painted an optimistic future for the expanding EV field.
Enhanced Anticorrosion and Antifouling Properties of Lubricant‐Infused Pyramidal Polydimethylsiloxane Coating
Authors: Li Dandan , Zhu Jiahui , Yu Jing , Liu Jingyuan , Chen Rongrong , Liu Qi , Mei Li , Lin Zaiwen , Wang Jun 
Published: October 2022

AbstractAlthough slippery liquid‐infused porous surfaces (SLIPSs) have been extensively studied for anticorrosion and antifouling applications, their durability and stability restrict their practicality. Herein, a technique is presented for fabricating SLIPS with dual‐protection. First, a polydimethylsiloxane (PDMS) coating with a pyramidal morphology is fabricated through mold‐based lithographic techniques. The mold is prepared by applying a texturing process to a monocrystalline silicon wafer of the type used to produce solar cells. Once infused with lubricant, the SLIPS is prepared and has a water‐contact angle of 112°, on an inclined surface, water droplets slide easily off its surface. Due to the excellent chemical stability and low superficiality of the lubricant layer, the SLIPS offers excellent antifouling and anticorrosion properties. In a static marine environment, the SLIPS has an extremely low coverage fraction of algae. After being immersed in a 3.5% NaCl solution for 400 days, |Z|0.01 Hz ≈ 1011 Ω, and the phase angle at high frequency is approximately −90°. SLIPSs based on the PDMS coating provide dual protection (anticorrosion and antifouling), showing that this design approach produces SLIPSs with excellent potential for applications in marine environments.
Dockground resource for protein recognition studies
Authors: Collins Keeley W. , Copeland Matthew M. , Kotthoff Ian , Singh Amar , Kundrotas Petras J. , Vakser Ilya A. 
Published: October 2022

AbstractStructural information of protein–protein interactions is essential for characterization of life processes at the molecular level. While a small fraction of known protein interactions has experimentally determined structures, computational modeling of protein complexes (protein docking) has to fill the gap. The Dockground resource (http://dockground.compbio.ku.edu) provides a collection of datasets for the development and testing of protein docking techniques. Currently, Dockground contains datasets for the bound and the unbound (experimentally determined and simulated) protein structures, model–model complexes, docking decoys of experimentally determined and modeled proteins, and templates for comparative docking. The Dockground bound proteins dataset is a core set, from which other Dockground datasets are generated. It is devised as a relational PostgreSQL database containing information on experimentally determined protein–protein complexes. This report on the Dockground resource describes current status of the datasets, new automated update procedures and further development of the core datasets. We also present a new Dockground interactive web interface, which allows search by various parameters, such as release date, multimeric state, complex type, structure resolution, and so on, visualization of the search results with a number of customizable parameters, as well as downloadable datasets with predefined levels of sequence and structure redundancy.
A case of ectopic cervical thymoma with myasthenia gravis mimicking a parathyroid tumour
Authors: Kamimura Go , Ueda Kazuhiro , Takeda Aya , Miyata Ryo , Aoki Masaya , Nagata Toshiyuki , Sato Masami 
Published: October 2022

AbstractReports of cervical thymoma with myasthenia gravis are rare. In addition, 99mTc‐MIBI (methoxyisobutylisonitrile:sestamibi) scintigraphy is a useful diagnostic examination for enlarged parathyroid tumours; however, there are a few reports of its accumulation in thymoma. Among them, there are no reports of cervical thymomas with 99mTc‐MIBI accumulation complicated by myasthenia gravis. In this study, we performed surgery on a patient with preoperative myasthenic crisis accompanied by a cervical thymoma and a parathyroid tumour. Preoperatively, the cervical mass was determined to be a parathyroid tumour and was complicated by myasthenia gravis without thymic tumour. However, a pathological examination revealed that the cervical tumour with 99mTc‐MIBI accumulation was a Type B2 thymoma, and a parathyroid tumour was identified in the vicinity. We report a very rare case in which symptoms improved with surgery.
Sodium titanate nanowires for Na+‐based hybrid energy storage with high power density
Authors: Jinghan Zuo , Zeyu Liu , Huaning Jiang , Qian Chen , Zhilin Yang , Xiaokang Gu , Yuying Jiao , Yongji Gong 
Published: October 2022

AbstractIt is crucial to enhance the rate capability of the titanium‐based materials for fulfilling their promising potential as the anode materials of sodium‐ion batteries (SIBs). Herein, Mn‐doped sodium titanate (Mn‐NTO) nanowires with homogeneously distributed ultrathin carbon nanosheets (Mn‐NTO@C) are synthesized by a one‐step salt‐template‐assisted method, showing much‐enhanced power density. The as‐prepared Mn‐NTO@C demonstrates the realization of hybrid energy storage, which reconciles the diffusion‐controlled behavior with the pseudocapacitive‐controlled behavior. It has been revealed that the Mn heteroatoms can raise the proportion of Na2Ti3O7 phase with the expanded crystal lattice, facilitating the diffusion‐controlled insertion/extraction process of sodium ions. Meanwhile, the hybrid morphology of Mn‐NTO nanowires and carbon nanosheets provides a promoted structure stability. As a result, the assembled Na||Mn‐NTO@C half‐cells work well at an extreme current density of 24 A g−1 for 10 000 cycles with a capacity retention of 95.2%. Moreover, the Mn‐NTO@C||Na3V2(PO4)3 (NVP) full cells exhibit an attenuation of only 0.0015% per cycle at 20 A g−1 for over 10 000 cycles, and the energy density and power density of the full cells reach an ultrahigh level of 262 Wh kg−1 and 16.3 kW kg−1, respectively.
Revealing the contribution of GbPR10.5D1 to resistance against Verticillium dahliae and its regulation for structural defense and immune signaling
Authors: Guo Jin , Cao Peihua , Yuan Leitian , Xia Guixian , Zhang Huanyang , Li Jing , Wang Fuxin 
Published: October 2022

AbstractAs an important family of pathogenesis‐related (PR) proteins, the functional diversification and roles of PR10s in biotic stress have been well documented. However, the molecular basis of PR10s in plant defense responses against pathogens remains to be further understood. In the present study, we analyzed the phylogenetic relationship and function of a novel PR10 named GbPR10.5D1 in Sea‐Island (or Pima or Egyptian) cotton (Gossypium barbadense L.), which has been identified as a Verticillium dahliae Kleb.‐induced protein in a previous proteomics study. Phylogenetic analysis revealed that GbPR10.5D1, located on chromosome 2, is a unique member of GbPR10. The expression of GbPR10.5D1 was preferably in the root and induced upon V. dahliae infection. GbPR10.5D1 proteins were distributed in both nucleus and cytoplasm. GbPR10.5D1‐virus‐induced gene‐silencing (VIGS) cotton plants were more susceptible to infection by V. dahliae, whereas overexpression (OE) of GbPR10.5D1 in cotton enhanced the resistance. By comparative transcriptome analysis between GbPR10.5D1‐OE and wild‐type (WT) plants and quantitative real‐time polymerase chain reaction (qRT‐PCR) verification, we found transcriptional activation of genes involved in cutin, suberine, and wax biosynthesis and mitogen‐activated protein kinase (MAPK) signaling under normal conditions. Upon pathogen infection, defense signaling, fatty acid degradation, and glycerolipid metabolism were specifically activated in GbPR10.5D1‐OE plants; biological processes (BPs), including glycolysis and gluconeogenesis, DNA replication, and cell wall organization, were specifically repressed in WT plants. Collectively, we proposed that GbPR10.5D1 possibly mediated lipid metabolism pathway to strengthen structural defense and activate defense signaling, which largely released the repression of cell growth caused by V. dahliae infection.