bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oncologic outcomes of segmentectomy for stage IA radiological solid-predominant lung cancer &gt;2 cm in maximum tumour size
Authors: Hattori Aritoshi , Matsunaga Takeshi , Fukui Mariko , Takamochi Kazuya , Oh Shiaki , Suzuki Kenji 
Published: September 2022

AbstractOBJECTIVESWe aimed to compare the outcomes of segmentectomy with those of lobectomy in clinical-stage IA radiological solid-predominant non-small-cell lung cancer (NSCLC) &gt;2 cm in maximum tumour size.METHODSA retrospective review was performed for radiological solid-predominant NSCLC &gt;2–3 cm in maximum tumour size with a ground-glass opacity component on thin-section computed tomography. Multivariable or propensity score-matched analyses were performed to control for confounders for survival. Overall survival (OS) was analysed using a Kaplan–Meier estimation.RESULTSOf the 215 eligible cases, segmentectomy and lobectomy were performed in 46 and 169 patients. Multivariable analysis revealed that standardized uptake value (hazard ratio: 1.148, 95% confidence interval: 1.032–1.276, P = 0.011) was an independently significant prognosticators of OS, while the operative mode was not associated (hazard ratio: 0.635, 95% confidence interval: 0.132–3.049, P = 0.570). The 5 y-OS was excellent and did not differ significantly between segmentectomy and lobectomy (95.5% vs 90.2%; P = 0.697), which was also shown in the propensity score analysis (96.8% vs 94.0%; P = 0.406), with a median follow-up time of 5.2 years. Locoregional recurrence was found in 2 (4.3%) segmentectomy and 13 (7.7%) lobectomy (P = 0.443). In the subgroup analysis stratified by solid component size, the 5 y-OS was similar between segmentectomy and lobectomy in the c-T1b and c-T1c groups, respectively [c-T1b (n = 163): 94.1% vs 91.8%; P = 0.887 and c-T1c (n = 52): 100% vs 84.9%; P = 0.197].CONCLUSIONSSegmentectomy showed similar oncological results compared to lobectomy in solid-predominant NSCLC with a ground-glass opacity component &gt;2–3 cm in maximum tumour size. More prospective randomized trials are needed to adequately expand the indication of anatomic segmentectomy for early-stage NSCLC.
mtDNA heteroplasmy gives rise to a new maternal lineage in North Pacific humpback whales (Megaptera novaeangliae)
Authors: Pierszalowski Sophie P , Steel Debbie J , Gabriele Christine M , Neilson Janet L , Vanselow Phoebe B S , Cedarleaf Jennifer A , Straley Janice M , Baker C Scott 
Published: September 2022

AbstractHeteroplasmy in the mitochondrial genome offers a rare opportunity to track the evolution of a newly arising maternal lineage in populations of non-model species. Here, we identified a previously unreported mitochondrial DNA haplotype while assembling an integrated database of DNA profiles and photo-identification records from humpback whales in southeastern Alaska (SEAK). The haplotype, referred to as A8, was shared by only 2 individuals, a mature female with her female calf, and differed by only a single base pair from a common haplotype in the North Pacific, referred to as A−. To investigate the origins of the A8 haplotype, we reviewed n = 1,089 electropherograms (including replicate samples) of n = 710 individuals with A− haplotypes from an existing collection. From this review, we found 20 individuals with clear evidence of heteroplasmy for A−/A8 (parental/derived) haplotypes. Of these, 15 were encountered in SEAK, 4 were encountered on the Hawaiian breeding ground (the primary migratory destination for whales in SEAK), and 1 was encountered in the northern Gulf of Alaska. We used genotype exclusion and likelihood to identify one of the heteroplasmic females as the likely mother of the A8 cow and grandmother of the A8 calf, establishing the inheritance and germ-line fixation of the new haplotype from the parental heteroplasmy. The mutation leading to this heteroplasmy and the fixation of the A8 haplotype provide an opportunity to document the population dynamics and regional fidelity of a newly arising maternal lineage in a population recovering from exploitation.
SN 2020wnt: a slow-evolving carbon-rich superluminous supernova with no O ii lines and a bumpy light curve
Authors: Gutiérrez C P , Pastorello A , Bersten M , Benetti S , Orellana M , Fiore A , Karamehmetoglu E , Kravtsov T , Reguitti A , Reynolds T M , Valerin G , Mazzali P , Sullivan M , Cai Y-Z , Elias-Rosa N , Fraser M , Hsiao E Y , Kankare E , Kotak R , Kuncarayakti H , Li Z , Mattila S , Mo J , Moran S , Ochner P , Shahbandeh M , Tomasella L , Wang X , Yan S , Zhang J , Zhang T , Stritzinger M D 
Published: September 2022

ABSTRACT
We present the analysis of SN 2020wnt, an unusual hydrogen-poor superluminous supernova (SLSN-I), at a redshift of 0.032. The light curves of SN 2020wnt are characterized by an early bump lasting ∼5 d, followed by a bright main peak. The SN reaches a peak absolute magnitude of M$_{r}^{\rm max}=-20.52\pm 0.03$ mag at ∼77.5 d from explosion. This magnitude is at the lower end of the luminosity distribution of SLSNe-I, but the rise-time is one of the longest reported to date. Unlike other SLSNe-I, the spectra of SN 2020wnt do not show O ii, but strong lines of C ii and Si ii are detected. Spectroscopically, SN 2020wnt resembles the Type Ic SN 2007gr, but its evolution is significantly slower. Comparing the bolometric light curve to hydrodynamical models, we find that SN 2020wnt luminosity can be explained by radioactive powering. The progenitor of SN 2020wnt is likely a massive and extended star with a pre-SN mass of 80 M⊙ and a pre-SN radius of 15 R⊙ that experiences a very energetic explosion of 45 × 1051 erg, producing 4 M⊙ of 56Ni. In this framework, the first peak results from a post-shock cooling phase for an extended progenitor, and the luminous main peak is due to a large nickel production. These characteristics are compatible with the pair-instability SN scenario. We note, however, that a significant contribution of interaction with circumstellar material cannot be ruled out.
Ursolic acid-piperazine-dithiocarbamate ruthenium(II) polypyridyl complexes induced necroptosis in MGC-803 cells
Authors: Jiang Hong , Wei Jian-Hua , Lin Cui-Yan , Liang Gui-Bin , He Rui-Jie , Huang Ri-Zhen , Ma Xian-Li , Huang Guo-Bao , Zhang Ye 
Published: September 2022

Abstract
Three ursolic acid-piperazine-dithiocarbamate ruthenium(II) polypyridyl complexes Ru1–Ru3 were designed and synthesized for evaluating antitumor activity. All the complexes exhibited high in vitro cytotoxicity against MGC-803, T24, HepG2, CNE2, MDA-MB-231, MCF-7, A549, and A549/DDP cell lines. Ru1, Ru2, and Ru3 were 11, 8 and 10 times, respectively, more active than cisplatin against A549/DDP. An in vivo study on MGC-803 xenograft mouse models demonstrated that representative Ru2 exhibited an effective inhibitory effect on tumor growth, showing stronger antitumor activity than cisplatin. Biological investigations suggested that Ru2 entered MGC-803 cells by a clathrin-mediated endocytic pathway, initially localizing in the lysosomes and subsequently escaping and localizing in the mitochondria. Mitochondrial swelling resulted in vacuolization, which induced vacuolation-associated cell death and necroptosis with the formation of necrosomes (RIP1–RIP3) and the uptake of propidium iodide. These results demonstrate that the potential of Ru2 as a chemotherapeutic agent to kill cancer cells via a dual mechanism represents an alternative way to eradicate apoptosis-resistant forms of cancer.
MTM1 displays a new function in the regulation of nickel resistance in Saccharomyces cerevisiae
Authors: Xu Naifeng , Xu Yuan , Smith Nathan , Chen Huizhu , Guo Ziguo , Lee Jaekwon , Wu Xiaobin 
Published: September 2022

Abstract
Nickel (Ni) is an essential yet toxic trace element. Although a cofactor for many metalloenzymes, nickel function and metabolism is not fully explored in eukaryotes. Molecular biology and metallomic methods were utilized to explore the new physiological functions of nickel in Saccharomyces cerevisiae. Here we showed that MTM1 knockout cells displayed much stronger nickel tolerance than wild-type cells and mitochondrial accumulations of Ni and Fe of mtm1Δ cells dramatically decreased compared to wild-type cells when exposed to excess nickel. Superoxide dismutase 2 (Sod2p) activity in mtm1Δ cells was severely attenuated and restored through Ni supplementation in media or total protein. SOD2 mRNA level of mtm1Δ cells was significantly higher than that in the wild-type strain but was decreased by Ni supplementation. MTM1 knockout afforded resistance to excess nickel mediated through reactive oxygen species levels. Meanwhile, additional Ni showed no significant effect on the localization of Mtm1p. Our study reveals the MTM1 gene plays an important role in nickel homeostasis and identifies a novel function of nickel in promoting Sod2p activity in yeast cells.
The chromatin accessibility landscape of pistils and anthers in rice
Authors: Wang Guanqun , Li Xiaozheng , Shen Wei , Li Man-Wah , Huang Mingkun , Zhang Jianhua , Li Haoxuan 
Published: September 2022

Abstract
Transcription activation is tightly associated with the openness of chromatin and allows direct contact between transcriptional regulators and their targeted DNA for gene expression. However, there are limited studies on the annotation of open chromatin regions (OCRs) in rice (Oryza sativa), especially those in reproductive organs. Here, we characterized OCRs in rice pistils and anthers with an assay for transposase-accessible chromatin using sequencing. Despite a large overlap, we found more OCRs in pistils than in anthers. These OCRs were enriched in gene transcription start sites (TSSs) and showed tight associations with gene expression. Transcription factor (TF) binding motifs were enriched at these OCRs as validated by TF chromatin immunoprecipitation followed by sequencing. Pistil-specific OCRs provided potential regulatory networks by binding directly to the targets, indicating that pistil-specific OCRs may be indicators of cis-regulatory elements in regulating pistil development, which are absent in anthers. We also found that open chromatin of pistils and anthers responded differently to low temperature (LT). These data offer a comprehensive overview of OCRs regulating reproductive organ development and LT responses in rice.
Arabidopsis plastid carbonic anhydrase βCA5 is important for normal plant growth
Authors: Weerasooriya Hiruni N , DiMario Robert J , Rosati Viviana C , Rai Ashwani K , LaPlace Lillian M , Filloon Victoria D , Longstreth David J , Moroney James V 
Published: September 2022

Abstract
Carbonic anhydrases (CAs) are zinc-metalloenzymes that catalyze the interconversion of CO2 and HCO3−. In heterotrophic organisms, CAs provide HCO3− for metabolic pathways requiring a carboxylation step. Arabidopsis (Arabidopsis thaliana) has 14 α- and β-type CAs, two of which are plastid CAs designated as βCA1 and βCA5. To study their physiological properties, we obtained knock-out (KO) lines for βCA1 (SALK_106570) and βCA5 (SALK_121932). These mutant lines were confirmed by genomic PCR, RT-PCR, and immunoblotting. While βca1 KO plants grew normally, growth of βca5 KO plants was stunted under ambient CO2 conditions of 400 µL L−1; high CO2 conditions (30,000 µL L−1) partially rescued their growth. These results were surprising, as βCA1 is more abundant than βCA5 in leaves. However, tissue expression patterns of these genes indicated that βCA1 is expressed only in shoot tissue, while βCA5 is expressed throughout the plant. We hypothesize that βCA5 compensates for loss of βCA1 but, owing to its expression being limited to leaves, βCA1 cannot compensate for loss of βCA5. We also demonstrate that βCA5 supplies HCO3− required for anaplerotic pathways that take place in plastids, such as fatty acid biosynthesis.
Wolbachia wAlbB remains stable in Aedes aegypti over 15 years but exhibits genetic background-dependent variation in virus blocking
Authors: Liang Xiao , Tan Cheong Huat , Sun Qiang , Zhang Meichun , Wong Pei Sze Jeslyn , Li Meizhi Irene , Mak Keng Wai , Martín-Park Abdiel , Contreras-Perera Yamili , Puerta-Guardo Henry , Manrique-Saide Pablo , Ng Lee Ching , Xi Zhiyong 
Published: September 2022

Abstract
The ability of the maternally transmitted endosymbiotic bacterium Wolbachia to induce cytoplasmic incompatibility (CI) and virus blocking makes it a promising weapon for combatting mosquito-borne diseases through either suppression or replacement of wild-type populations. Recent field trials show that both approaches significantly reduce the incidence of dengue fever in humans. However, new questions emerge about how Wolbachia-mosquito associations will co-evolve over time and whether Wolbachia-mediated virus blocking will be affected by the genetic diversity of mosquitoes and arboviruses in the real world. Here, we have compared the Wolbachia density and CI expression of two wAlbB-infected Aedes aegypti lines transinfected 15 years apart. We have also assessed wAlbB-mediated virus blocking against dengue (DENV), Zika (ZIKV), and Chikungunya (CHIKV) viruses and examined whether host genetic backgrounds modulate viral blocking effects by comparing ZIKV infection in mosquitoes with a Mexican genetic background to those with a Singaporean background. Our results show that over 15 years, wAlbB maintained the capacity to form a stable association with Ae. aegypti in terms of both density and CI expression. There were variations in wAlbB-induced virus blocking against CHIKV, DENV, and ZIKV, and higher inhibitory effects on ZIKV in mosquitoes on the Singaporean genetic background than on the Mexican background. These results provide important information concerning the robustness and long-term stability of Wolbachia as a biocontrol agent for arbovirus disease control.
Regionalism against Centralization: Resistance to Federal Education after the Mexican Revolution
Authors: Cabal Manuel 
Published: September 2022

AbstractWhy are states in the developing world able to centralize policy regulation and implementation in some places inside their territory but not in others? I argue that uneven state capacity in periods of centralization is a function of the political resources that regional politicians have to resist the advancement of central institutions. These resources include cohesion against the center and independent popular support. I analyze the expansion of primary education after the Mexican Revolution in four subnational cases representing scenarios of regional resistance to institutional centralization and compare the framework to alternative explanations. The article contributes to evaluating the causes of “state weakness” in developing countries and understanding the politics of institutional choice under federal constitutions.
Refusing and Redressing Gestational Labor: A Social Reproduction Account of Abortion
Authors: Bryson Janna 
Published: September 2022

Abstract
This article analyzes abortion politics from a Marxist feminist perspective by adapting social reproduction theory (SRT). Despite the urgent threats to abortion access facing feminists worldwide, abortion is largely absent from contemporary SRT. This absence is first addressed from a theoretical perspective through the development of three arguments: that SRT can connect abortion to the biological reproduction of labor power via gestational labor, that abortion can make gestational labor less alienating, and that abortion can function as a refusal of productivism and reproductive sexuality. The article then applies these arguments to a preexisting case study of reproductive justice and women in Canada’s Seasonal Agricultural Worker Program. By situating abortion within migrant workers’ struggles over social reproduction processes, this analysis demonstrates that SRT is useful for the study of abortion and can generate insights different from those of the reproductive justice approach alone.
Single-stage Layered versus Nonlayered Mastopexy with Augmentation in Muscle Splitting Biplane: A Comparative Analysis
Authors: Khan Umar D. 
Published: September 2022

Background:
Single-stage layered mastopexy with augmentation is a modification of muscle-splitting pocket that reduces risk of complications and revision rate.

Methods:
A retrospective chart review of all the consecutive cases, performed by a single surgeon, from March 2007 to August 2019, was conducted. All patients had surgery with superomedial pedicle in an approved facility. The patients were divided into group A whose implants were placed through the mastopexy incision, and group B whose implants were placed first and access closed prior to mastopexy.

Results:
Nonlayered group A included 116 patients and layered group B included 102 patients. Groups A and B had a mean age of 32.3 + 9.70 and 34.8 + 10.39 years (range, 20–66), respectively (P value 0.070). In groups A and B, 95 (81.9%) and 74 (72.5%) patients underwent primary and 21 (18.1%) and 28 (27.5%) patients underwent secondary procedures, respectively (P value 0.099). There were no hematomas in group A and two patients had hematoma in group B (P value 0.218). In group A, one patient experienced periprosthetic infection with none in group B (P value 0.41). Wound breakdown was observed in ten (8.6%) patients in group A and four (3.9%) patients in group B (P value 0.128). In group A, there was a partial or complete nipple loss in two (1.7%) and none in group B (P value 0.412). In group A, 15 (12.9%) underwent revision surgery and six (6.5%) in group B (P value 0.127).

Conclusion:
Layered mastopexy with augmentation provides improved safety, fewer complications and revisions with adequate results.
Dose-response relationship between epidural bupivacaine dose and mortality risk after surgical resection of nonsmall-cell lung cancer
Authors: Wu Hsiang-Ling , Tai Ying-Hsuan , Li Chun-Cheng , Cata Juan P. , Wang Chien-Wun , Chang Kuang-Yi , Tsou Mei-Yung , Lin Shih-Pin 
Published: September 2022

Background:
Preclinical studies have shown that local anesthetics may modify the growth and invasion of cancer cells. However, few clinical studies have evaluated their impact on cancer outcomes after tumor resection.

Methods:
In this single-center cohort study, patients who underwent surgical resection of stage IA through IIIB nonsmall-cell lung cancer and used patient-controlled epidural analgesia from 2005 to 2015 were recruited and followed until May 2017. Data of the epidural bupivacaine dose for each patient were obtained from infusion pump machines. Proportional hazards regression models were used to analyze the associations between bupivacaine dose with postoperative cancer recurrence and all-cause mortality.

Results:
A total of 464 patients were analyzed. Among these patients, the mean bupivacaine dose was 352 mg (± standard deviation 74 mg). After adjusting for important clinical and pathological covariates, a significant dose-response relationship was observed between epidural bupivacaine dose and all-cause mortality (adjusted hazard ratio: 1.008, 95% confidence interval: 1.001–1.016, p = 0.029). The association between bupivacaine dose and cancer recurrence were not significant (adjusted hazard ratio: 1.000, 95% confidence interval: 0.997–1.002, p = 0.771). Age, sex, body mass index, mean daily maximum pain score, and pathological perineural infiltration were independently associated with bupivacaine dose.

Conclusion:
A dose-dependent association was found between epidural bupivacaine dose and long-term mortality among patients following surgical resection of nonsmall-cell lung cancer. Our findings do not support the hypothetical anticancer benefits of local anesthetics. More studies are needed to elucidate the role of local anesthetics in cancer treatment.
Association of glutathione S-transferases (GSTT1, GSTM1 and GSTP1) genes polymorphisms with nonalcoholic fatty liver disease susceptibility: A PRISMA-compliant systematic review and meta-analysis
Authors: Zhu Yi , Yang Jian-Hua , Hu Jun-Ping , Qiao Ming 
Published: September 2022

Background:
Glutathione S-transferases (GSTs) genes single-nucleotide polymorphisms (SNPs) have been connected with the susceptibility of nonalcoholic fatty liver disease (NAFLD), but with inconsistent results across the current evidences. The present work was schemed to explore the association between GSTs genes polymorphisms and the NAFLD vulnerability via meta-analysis.

Methods:
PubMed, Web of Science, Cochrane Library, China National Knowledge Infrastructure and Wanfang were retrieved for eligible literatures previous to March 10, 2021. The odds ratio (OR) of the dichotomic variables and the standardized mean difference of quantitative variables with corresponding 95% confidence intervals (95%CIs) were computed to evaluate the strength of the associations. The quality of included studies were assessed via using Newcastle-Ottawa Scale (NOS).

Results:
In total, 7 case-control studies encompassing 804 NAFLD patients and 1362 disease-free controls in this meta-analysis. Ultimately, this analysis included 6, 5 and 5 studies for GSTM1, GSTT1 and GSTP1 polymorphisms, respectively. The pooled data revealed that the GSTs genes SNPs had conspicuous associations with NAFLD susceptibility: for GSTM1, null versus present, OR = 1.46, 95%CI 1.20 to 1.79, P = .0002; for GSTT1, null versus present, OR = 1.34, 95%CI 1.06 to 1.68, P = .01; for GSTP1, Ile/Val or Val/Val versus Ile/Ile, OR = 1.60, 95%CI 1.23 to 2.09, P = .0005.

Conclusion:
This work revealed that the GSTM1 null, GSTT1 null and GSTP1-Val genotypes might be related to increased NAFLD susceptibility.
Auricular Reconstruction in Adult Patients With Unprepared Congenital Microtia
Authors: Ma Tengxiao , Li Lei , Wang Xiu , Zhang Zhengwen 
Published: September 2022

Background
Because costal cartilage becomes hard and brittle, skin thickens, and elasticity decreases in adult patients with microtia, it is a great challenge for plastic surgeons to reconstruct the auricle with autologous costal cartilage. Therefore, this article concentrates on sharing the experience of auricular reconstruction in adult patients with microtia.

Methods
From January 2015 to January 2021, auricular reconstruction was performed in 38 adult patients (39 ears) of congenital microtia based on autologous costal cartilage. The whole procedure was divided into 2 stages: stage I, the individualized framework carved with autologous costal cartilage was inserted into subcutaneous pocket in the mastoid region; then, the earlobe was transposed backward; and stage II, ear elevation, harvesting the retroauricular fascial flap to cover the support scaffold and closing the defect with free skin graft, was performed.

Results
All patients successfully underwent ear reconstruction. The follow-up time ranged from 3 months to 3 years. Infection occurred in 1 patient. The ear frameworks were partially broken at the helix in 4 cases. Retroauricular graft skin survival was poor in 1 patient. Retroauricular hypertrophic scars occurred in 2 cases. Bad projection of the reconstructed ear occurred in 1 case. Totally 38 patients were satisfied with the results.

Conclusions
According to the physiological characteristics of the costal cartilage and skin soft tissues of adult patients, improvements are made to details based on the Nagata's method, so that the adult patients with microtia can obtain satisfactory surgical results.