bluuebunny/crossref_metadata_2025_split huggingface dataset:
Theoretical prediction of doping iron ions effect on the optoelectronic and magnetic cobalt-based perovskite (Co1-xFexScO3)
Authors: Alsmadi Amjad W , Mahmoud Nada T , Mousa Ahmad A , Abu-Jafar Mohammed S , Abusaimeh Hesham 
Published: September 2022

Abstract
The ab-initio framework has taken place by means of the Full-potential Linear Augmented Plane-Wave (FP-LAPW) method, using the modified Becke-Johnson exchange potential approximation. This modified method was carried out to study the effects of doping iron ions on the structural, electronic, magnetic, and optical properties of CoScO3 perovskite compound. Doping Fe ions have formed a nonstoichiometric perovskite Co1-xFexScO3 where x = 0, 0.25, 0.50, 0.75, and 1. all doped compounds crystallized in a tetragonal lattice due to the Jahn-Teller effect, while the lattice constant was calculated and found to vary between the value of the two pure compounds. Meanwhile, the stabilities of the studied compounds are shown in the negative values of the formation energy. The magnetic moment was calculated and the whole compounds showed a ferromagnetic behavior. Co1-xFexScO3 perovskite is a potential candidate for spintronic application due to the presence of the half metallic behavior. All optical spectrums including dielectric function, absorption coefficient, reflectivity and refractivity index, and energy loss function show remarkable features for the proposed perovskites.
The diversity, structure and function of microbial communities changes across aging process of tobacco leaves
Authors: Wang Fan , Jin Yongming , Chen Xiaona , Zhang Yao , Jiang Xinglin , Zhang Ge , Chen Guoqiang , Yang Mingjun , Leng Feifan , Li Hongtao , Wu Lijun , Zhang Haibo 
Published: September 2022

Abstract
Microbial communities that inhabit aging tobacco leaves play a key role in improving products quality. A better understanding of microbial communities on the aging of tobacco leaves could provide an important microbial repository for the industrial applications. Here, we examined the structural and compositional changes of microbial communities throughout the aging process of by tobacco leaves 16 S and ITS rRNA amplicon sequencing techniques and identified the potential metabolic pathways of bacteria and fungi using Functional Annotation of Prokaryotic Taxa (FAPROTAX) and Fungi Functional Guild (FUNGuild), respectively. The results showed that the diversity and structure of the microbial communities keep changing along with the aging process went on. The richness and diversity of bacterial community decreased, while the richness of fungal community was in an inverse trend. At the phylum level, the bacterial community was dominated by Proteobacteria, Actinobacteria, and Firmicutes, while Ascomycota and Basidiomycota were the dominant species in the fungal community. In the bacterial community, metabolic functions related to the carbon and nitrogen cycles which response to the degradation of harmful components, and the metabolism of aromatic hydrocarbons showed extremely dynamic at different aging periods. The change of the main nutritional mode of the fungal community also led to an increase in the abundance of saprophytic fungi. These results provide information on the succession of microbial community structure and function in the whole process of tobacco aging and suggest that the aging process of tobacco leaves can be a natural microbial collection for target microorganism and their metabolites. It also enables the further investigation of coordination mechanisms between beneficial microbial regulation and pathogenicity during aging process.
Mesoporous indium tin oxide perovskite solar cells in bifacial configuration
Authors: Sohmer Maayan , Yudco Shir , Etgar Lioz 
Published: September 2022

Abstract
In this work, we present a fully printable mesoporous (mp) indium tin oxide (ITO) perovskite solar cell. The solar cell structure consists of triple-oxide screen-printed mp layers. In this structure, the perovskite does not form a separate layer but fills the pores of the triple-oxide structure. The perovskite is utilized as both a light harvester and a hole transporting material. One of the advantages of this solar cell structure is the transparent contact (mp ITO), which permits the use of this cell structure in a bifacial configuration without the need for additional layers or thinner counter electrodes. We performed photovoltaic (PV) measurements on both sides (i.e. ITO side and glass side), where the glass side shows 15.3% efficiency compared to 4.4% of the ITO side. Further study of the mechanism shows that the dominant mechanism when illuminating from the glass side is Shockley–Read–Hall recombination in the bulk, while illuminating from the ITO side shows recombination in multiple traps and inter-gap defect distribution, which explains the poor PV performance of the ITO side. Electrochemical impedance spectroscopy sheds more light on the resistance and capacitance. Finally, we demonstrate 18.3% efficiency in the bifacial configuration. This work presents a fully printable, large-scale suitable solar cell structure that can function in a bifacial configuration.
TEx-MST: tissue expression profiles of MANE select transcripts
Authors: Tung Kuo-Feng , Lin Wen-chang 
Published: September 2022

Abstract
Recently, a new reference transcript dataset [Matched Annotation from the NCBI and EMBL-EBI (MANE) select] was released by NCBI and EMBL-EBI to make available a new unified representative transcript for human protein-coding genes. While the main purpose of MANE project is to provide a harmonized gene and transcript information standard, there is no explicit tissue expression information about these MANE select transcripts. In this report, we tried to provide useful expression profiles of MANE select transcripts in various normal human tissues to allow further interrogation of their molecular modulations and functional significance. We obtained the new V9 transcript expression dataset from the Genotype-Tissue Expression (GTEx) web portal. This new GTEx dataset, based on a long-read sequencing platform, affords better assessment of the expression of alternative spliced transcripts. This tissue expression profiles of MANE select transcripts (TEx-MST) database not only provides the basic information of MANE select transcripts but also tissue expression profiles on alternative transcripts in protein-coding genes. Users can initiate the interrogation by gene symbol searches or by browsing the MANE genes with various criteria (such as genome locations or expression rankings). We further utilized the GENCODE biotype feature to identify the top-ranked protein-coding transcripts by choosing the most expressed protein-coding transcripts from GTEx datasets (both V8 and V9 datasets). In summary, there are 18 083 genes matched between MANE and GTEx. Among them, 13 245 MANE select transcripts matched with the top-ranked protein-coding transcripts in GTEx V9 dataset, which underlined the dominate expression of MANE select transcripts. This TEx-MST web bioinformatic database provides a visualized user interface for the normal tissue expression patterns of MANE select transcripts using the newly released GTEx dataset.
Database URL: TEx-MST is available at https://texmst.ibms.sinica.edu.tw/
Subclinical leaflet thrombosis after transcatheter aortic valve implantation is associated with silent brain injury on brain magnetic resonance imaging
Authors: Apor Astrid , Bartykowszki Andrea , Szilveszter Bálint , Varga Andrea , Suhai Ferenc I , Manouras Aristomenis , Molnár Levente , Jermendy Ádám L , Panajotu Alexisz , Turáni Mirjam Franciska , Papp Roland , Karády Júlia , Kolossváry Márton , Kováts Tímea , Maurovich-Horvat Pál , Merkely Béla , Nagy Anikó Ilona 
Published: September 2022

Abstract

Aims
Whether hypoattenuated leaflet thickening (HALT) following transcatheter aortic valve implantation (TAVI) carries a risk of subclinical brain injury (SBI) is unknown. We investigated whether HALT is associated with SBI detected on magnetic resonance imaging (MRI), and whether post-TAVI SBI impacts the patients’ cognition and outcome.

Methods and results
We prospectively enrolled 153 patients (age: 78.1 ± 6.3 years; female 44%) who underwent TAVI. Brain MRI was performed shortly post-TAVI and 6 months later to assess the occurrence of acute silent cerebral ischaemic lesions (SCIL) and chronic white matter hyperintensities (WMH). HALT was screened by cardiac computed tomography (CT) angiography (CTA) 6 months post-TAVI. Neurocognitive evaluation was performed before, shortly after and 6 months following TAVI. At 6 months, 115 patients had diagnostic CTA and 10 had HALT. HALT status, baseline, and follow-up MRIs were available in 91 cases. At 6 months, new SCIL was evident in 16%, new WMH in 66%. New WMH was more frequent (100 vs. 62%; P = 0.047) with higher median volume (319 vs. 50 mm3; P = 0.039) among HALT-patients. In uni- and multivariate analysis, HALT was associated with new WMH volume (beta: 0.72; 95%CI: 0.2–1.39; P = 0.009). The patients’ cognitive trajectory from pre-TAVI to 6 months showed significant association with the 6-month SCIL volume (beta: −4.69; 95%CI: −9.13 to 0.27; P = 0.038), but was not related to the presence or volume of new WMH. During a 3.1-year follow-up, neither HALT [hazard ratio (HR): 0.86; 95%CI: 0.202–3.687; P = 0.84], nor the related WMH burden (HR: 1.09; 95%CI: 0.701–1.680; P = 0.71) was related with increased mortality.

Conclusions
At 6 months post-TAVI, HALT was linked with greater WMH burden, but did not carry an increased risk of cognitive decline or mortality over a 3.1-year follow-up (NCT02826200).
Use of a pharmacy-based GP video consultation service: a mixed methods study
Authors: Parsons Joanne , Rahman Sameur , Bryce Carol , Atherton Helen 
Published: September 2022

AbstractBackgroundLittle is known about private general practice appointment services offered via video. This study aimed to explore which patients are using a video pharmacy-based general practitioner (GP) appointment service, including patterns of use, reasons for using the service, and satisfaction with the service.MethodsDescriptive statistics and parametric and nonparametric tests were used to conduct a retrospective cross-sectional analysis of routinely collected data on consultations, and postconsultation questionnaires. Interviews were conducted with patients and GPs.ResultsA total of 7,928 consultations were included in the analysis. More than half of appointments were booked for the same day, with lunchtime appointments being popular. The most common health condition was respiratory conditions, and 9% of consultations were used by patients using the service more than once. At least one prescription was issued in over half of all consultations. Overall, satisfactions of consultations were high.ConclusionsThe characteristics of those patients using the video consultation service match data on who uses online services in general practice. This study shows that some patients are willing to pay to use this private service because they feel it is more convenient, NHS services do not have capacity to see them at the time they need, or they do not have access to regular GP services.
Convergent structure with divergent adaptations in combinatorial microbiome communities
Authors: Taylor Megan , Janasky Lili , Vega Nic 
Published: September 2022

Abstract
Adaptation of replicate microbial communities frequently produces shared trajectories of community composition and structure. However, divergent adaptation of individual community members can occur and is associated with community-level divergence. The extent to which community-based adaptation of microbes should be convergent when community members are similar but not identical is, therefore, not well-understood. In these experiments, adaptation of combinatorial minimal communities of bacteria with the model host Caenorhabditis elegans produces structurally similar communities over time, but with divergent adaptation of member taxa and differences in community-level resistance to invasion. These results indicate that community-based adaptation from taxonomically similar starting points can produce compositionally similar communities that differ in traits of member taxa and in ecological properties.
ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet β-Cell
Authors: Rohli Kristen E , Boyer Cierra K , Bearrows Shelby C , Moyer Marshall R , Elison Weston S , Bauchle Casey J , Blom Sandra E , Zhang Jianchao , Wang Yanzhuang , Stephens Samuel B 
Published: September 2022

Abstract
Defects in the pancreatic β-cell’s secretion system are well-described in type 2 diabetes (T2D) and include impaired proinsulin processing and a deficit in mature insulin-containing secretory granules; however, the cellular mechanisms underlying these defects remain poorly understood. To address this, we used an in situ fluorescent pulse-chase strategy to study proinsulin trafficking. We show that insulin granule formation and the appearance of nascent granules at the plasma membrane are decreased in rodent and cell culture models of prediabetes and hyperglycemia. Moreover, we link the defect in insulin granule formation to an early trafficking delay in endoplasmic reticulum (ER) export of proinsulin, which is independent of overt ER stress. Using a ratiometric redox sensor, we show that the ER becomes hyperoxidized in β-cells from a dietary model of rodent prediabetes and that addition of reducing equivalents restores ER export of proinsulin and insulin granule formation and partially restores β-cell function. Together, these data identify a critical role for the regulation of ER redox homeostasis in proinsulin trafficking and suggest that alterations in ER redox poise directly contribute to the decline in insulin granule production in T2D.
This model highlights a critical link between alterations in ER redox and ER function with defects in proinsulin trafficking in T2D. Hyperoxidation of the ER lumen, shown as hydrogen peroxide, impairs proinsulin folding and disulfide bond formation that prevents efficient exit of proinsulin from the ER to the Golgi. This trafficking defect limits available proinsulin for the formation of insulin secretory granules during the development of T2D.
Advances and prospects of orchid research and industrialization
Authors: Zhang Diyang , Zhao Xue-Wei , Li Yuan-Yuan , Ke Shi-Jie , Yin Wei-Lun , Lan Siren , Liu Zhong-Jian 
Published: September 2022

Abstract
Orchidaceae is one of the largest, most diverse families in angiosperms with significant ecological and economical values. Orchids have long fascinated scientists by their complex life histories, exquisite floral morphology and pollination syndromes that exhibit exclusive specializations, more than any other plants on Earth. These intrinsic factors together with human influences also make it a keystone group in biodiversity conservation. The advent of sequencing technologies and transgenic techniques represents a quantum leap in orchid research, enabling molecular approaches to be employed to resolve the historically interesting puzzles in orchid basic and applied biology. To date, 16 different orchid genomes covering four subfamilies (Apostasioideae, Vanilloideae, Epidendroideae, and Orchidoideae) have been released. These genome projects have given rise to massive data that greatly empowers the studies pertaining to key innovations and evolutionary mechanisms for the breadth of orchid species. The extensive exploration of transcriptomics, comparative genomics, and recent advances in gene engineering have linked important traits of orchids with a multiplicity of gene families and their regulating networks, providing great potential for genetic enhancement and improvement. In this review, we summarize the progress and achievement in fundamental research and industrialized application of orchids with a particular focus on molecular tools, and make future prospects of orchid molecular breeding and post-genomic research, providing a comprehensive assemblage of state of the art knowledge in orchid research and industrialization.
Advancing women’s participation in climate action through digital health literacy: gaps and opportunities
Authors: Abdolkhani Robab , Choo Dawn , Gilbert Cecily , Borda Ann 
Published: September 2022

Abstract
Exploring the contribution of health informatics is an emerging topic in relation to addressing climate change, but less examined is a body of literature reporting on the potential and effectiveness of women participating in climate action supported by digital health. This perspective explores how empowering women through digital health literacy (DHL) can support them to be active agents in addressing climate change risk and its impacts on health and well-being. We also consider the current definitional boundary of DHL, and how this may be shaped by other competencies (eg, environmental health literacy), to strengthen this critical agenda for developed nations and lower-resource settings.
Structural insights into the substrate specificity of IMP-6 and IMP-1 metallo-β-lactamases
Authors: Yamamoto Keizo , Tanaka Hideaki , Kurisu Genji , Nakano Ryuichi , Yano Hisakazu , Sakai Hiromi 
Published: September 2022

Abstract
IMP-type metallo-β-lactamases confer resistance to carbapenems and a broad spectrum of β-lactam antibiotics. IMP-6 and IMP-1 differ by only a point mutation: Ser262 in IMP-1 and Gly262 in IMP-6. The kcat/Km values of IMP-1 for imipenem and meropenem are nearly identical; however, for IMP-6, the kcat/Km for meropenem is 7-fold that for imipenem. In clinical practice, this may result in an ineffective therapeutic regimen and, consequently, in treatment failure. Here, we report the crystal structures of IMP-6 and IMP-1 with the same space group and similar cell constants at resolutions of 1.70 and 1.94 Å, respectively. The overall structures of IMP-6 and IMP-1 are similar. However, the loop region (residues 60–66), which participates in substrate binding, is more flexible in IMP-6 than in IMP-1. This difference in flexibility determines the substrate specificity of IMP-type metallo-β-lactamases for imipenem and meropenem. The amino acid at position 262 alters the mobility of His263; this affects the flexibility of the loop via a hydrogen bond with Pro68, which plays the role of a hinge in IMP-type metallo-β-lactamases. The substitution of Pro68 with a glycine elicited an increase in the Km of IMP-6 for imipenem, whereas the affinity for meropenem remained unchanged.
Investment Facilitation and Sustainable Development: Insufficiencies and Improvements of ASEAN Investment Treaties
Authors: Chi Manjiao 
Published: September 2022

ABSTRACT
Investment facilitation has become a major topic in recent investment rule-making. Different approaches have been adopted to make international investment treaties (IITs) compatible with investment facilitation, including the laissez-faire approach, the patching-up approach, the orientation-shift approach, and the multilateralization approach. Investment facilitation and sustainable development are closely interrelated. Investment facilitation provisions (IFPs) in IITs are expected to contribute to both facilitating investment and promoting sustainable development. While the majority of ASEAN IITs, the RCEP in particular, contain a standalone investment facilitation clause that covers various IFPs, these IFPs are insufficiently effective in promoting sustainable development. It is advised that ASEAN states make further IIT-making and regulatory coordination efforts, especially at the regional level, to make ASEAN IITs more compatible with investment facilitation and sustainable development.
Paeonol protects against acute pancreatitis by Nrf2 and NF-κB pathways in mice
Authors: Zhang Peng , Yin Xing , Wang Xinxin , Wang Jiaqing , Na Guangning , Ирина Павловна Короткова 
Published: September 2022

Abstract

Objectives
Paeonol (PAE) is an active ingredient with anti-inflammatory and antioxidant properties. This study was designed to investigate the effect of PAE on acute pancreatitis (AP).

Methods
AP was induced by the intraperitoneal injection of 20% l-arginine (4 g/kg) for 6 h. Mice were pretreated with PAE (25, 50 or 100 mg/kg) intragastrically for 5 days. The histological damage and alterations of biochemical indicators, inflammatory cytokines and oxidative stress factors in AP mice were detected. The Nrf2 and NF-κB pathways were examined to illustrate the potential mechanism.

Key findings
In AP model, we found that PAE attenuated histological injury of pancreatic tissues, reduced the serum levels of α-amylase and increased Ca2+ contents in a dose-dependent manner. The white blood cell content, and IL-1β, IL-6 and TNF-α levels in the serum of AP mice were reduced by PAE. Furthermore, PAE caused a reduction of MPO and MDA levels, accompanied by an increase in SOD activity in the pancreas of AP mice. We also demonstrated that the alterations of Nrf2 and NF-κB pathways in AP mice were reversed by PAE.

Conclusions
PAE attenuates inflammation and oxidative stress in the development of AP by the regulation of Nrf2 and NF-κB pathways.
Role of jasmonate signaling in the regulation of plant responses to nutrient deficiency
Authors: Shikha Deep , Jakhar Pooja , Satbhai Santosh B 
Published: September 2022

AbstractPhytohormones regulate plant growth and development by integrating various internal developmental cues with external environmental conditions. Jasmonic acid (JA) is an oxylipin-derived phytohormone that modulates the plasticity of plant responses against fluctuating environmental cues. An increasing number of studies have shown that it regulates a wide spectrum of plant physiological and biochemical processes, including reproductive development, primary root growth, root hair development, seed germination, senescence, regeneration, defense against biotic stress such as pathogen infection and herbivory, and mitigation of a number of abiotic stresses such as salinity, drought, high and low temperatures, wounding, excessive UV exposure, limited water availability, and metal(oid)-induced toxicity. Nutrient deficiency is an abiotic stress that adversely affects plant growth, development, and productivity, and JA also plays an important role in regulation of these processes under such conditions. In this review, we summarize recent advances relating to the role of JA and its methyl ester derivative (methyl jasmonate) in modulating responses to nutrient deficiency, to the impact of nutrient status on JA biosynthesis and signaling, and to the crosstalk of JA with other phytohormones in shaping plant growth and development under deficiencies of various mineral elements.