bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lysine acetylation regulates antibiotic resistance in Escherichia coli
Authors: Fang Zuye , Lai Fubin , Cao Kun , Zhang Ziyuan , Cao Linlin , Liu Shiqin , Duan Yufeng , Yin Xingfeng , Ge Ruiguang , He Qing-Yu , Sun Xuesong 
Published: February 2022

ABSTRACTAntibiotic resistance is increasingly becoming a serious challenge to public health. The regulation of metabolism by post-translational modifications (PTMs) has been widely studied; however, the comprehensive mechanism underlying the regulation of acetylation in bacterial resistance against antibiotics is unknown. Herein, with Escherichia coli as the model, we performed quantitative analysis of the acetylated proteome of wild-type sensitive strain (WT) and ampicillin- (Re-Amp), kanamycin- (Re-Kan), and polymyxin B-resistant (Re-Pol) strains. Based on bioinformatics analysis combined with biochemical validations, we found that a common regulatory mechanism exists between the different resistant strains. Acetylation negatively regulates bacterial metabolism to maintain antibiotic resistance, but positively regulates bacterial motility. Further analyses revealed that key enzymes in various metabolic pathways were differentially acetylated. Particularly, pyruvate kinase (PykF), a key glycolytic enzyme regulating bacterial metabolism, and its acetylated form were highly expressed in the three resistant types and were identified as reversibly acetylated by the deacetylase CobB and the acetyl-transferase PatZ, and also could be acetylated by non-enzyme AcP in vitro. Further, the deacetylation of Lys413 of PykF increased the enzyme activity by changing the conformation of ATP binding site of PykF, resulting in an increase in energy production, which in turn increased the sensitivity of drug-resistant strains to antibiotics. This study provides novel insights for understanding bacterial resistance and lays the foundation for future research on regulation of acetylation in antibiotic-resistant strains.ImportanceThe misuse of antibiotics has resulted in an emergence of a large number of antibiotic-resistant strains, which seriously threaten human health. Bacterial metabolism is tightly controlled by protein post-translational modifications, especially acetylation. However, the comprehensive mechanism underlying regulation of acetylation in bacterial resistance remains unexplored. Here, acetylation was found to positively regulate bacterial motility and negatively regulate energy metabolism, which was common in all the different antibiotic-resistant strains. Moreover, the acetylation and deacetylation process of PykF was uncovered, and deacetylation of the Lys 413 of PykF was found to contribute to bacterial sensitivity to antibiotics. This study provides a new direction for research on development of bacterial resistance through post-translational modifications and provides a theoretical basis for the development of antibacterial drugs.
Population structure and genetic connectivity of the scalloped hammerhead shark (Sphyrna lewini) across nursery grounds from the Eastern Tropical Pacific: implications for management and conservation
Authors: Elizondo-Sancho Mariana , Rodríguez-Arriatti Yehudi , Albertazzi Federico J. , Bonilla-Salazar Adrián , Arauz Daniel , Arauz Randall , Areano Elisa , Avalos-Castillo Cristopher G. , Brenes Óscar , Chávez Elpis J. , Dominici-Arosemena Arturo , Espinoza Mario , Heidemeyer Maike , Tavares Rafael , Hernández Sebastián 
Published: February 2022

AbstractDefining demographically independent units and understanding gene flow between them is essential for managing and conserving exploited populations. The scalloped hammerhead shark, Sphyrna lewini, is a coastal semi-oceanic species found worldwide in tropical and subtropical waters. Pregnant females give birth in shallow coastal estuarine habitats that serve as nursery grounds for neonates and small juveniles, and adults move offshore and become highly migratory. We evaluated the population structure and connectivity of S. lewini in coastal areas across the Eastern Tropical Pacific (ETP) using both sequences of the mitochondrial DNA control region (mtCR) and nuclear-encoded microsatellite loci. The mtCR defined two genetically discrete geographic groups: the Mexican Pacific and the central-southern Eastern Tropical Pacific (Guatemala, Costa Rica, Panamá, and Colombia). Overall, the mtCR data showed low levels of haplotype diversity ranged from 0.000 to 0.608, while nucleotide diversity ranged from 0.000 to 0.0015. A more fine-grade population structure analysis was detected using microsatellite loci where Guatemala, Costa Rica, and Panamá differed significantly. Genetic diversity analysis with nuclear markers revealed an observed heterozygosity ranging from 0.68 to 0.71 and an allelic richness from 5.89 to 7.00. Relatedness analysis revealed that individuals within nursery areas were more closely related than expected by chance, suggesting that S. lewini may exhibit reproductive philopatric behaviour within the ETP. Findings of at least two different management units, and evidence of philopatric behaviour call for intensive conservation actions for this critically endangered species in the ETP.
Development of a physiological insulin resistance model in human stem cell-derived adipocytes
Authors: Friesen Max , Khalil Andrew S. , Barrasa M. Inmaculada , Jeppesen Jacob F. , Mooney David J. , Jaenisch Rudolf 
Published: February 2022

AbstractAdipocytes are key regulatory cells of human metabolism, and their dysfunction in insulin signaling is central to metabolic diseases such as type II diabetes mellitus (T2D). However, the progression of insulin resistance that leads to T2D is still poorly understood. This limited understanding is due, in part, to the dearth of suitable models of insulin signaling in human adipocytes. Traditionally, in vitro adipocyte models fail to recapitulate in vivo insulin signaling, possibly due to exposure to supraphysiological nutrient and hormone conditions. Here, we have developed a sensitization protocol for human pluripotent stem cell-derived adipocytes that uses physiologically relevant nutrient conditions to produce a potent signaling response comparable to in vivo adipocytes. After systematically optimizing conditions, this protocol allows for robust insulin-stimulated glucose uptake and transcriptional insulin response. Furthermore, exposure of these sensitized adipocytes to physiologically relevant hyperinsulinemic conditions dampens insulin-stimulated glucose uptake and dysregulates transcription of insulin-responsive genes. Overall, this sensitization methodology provides a novel platform for the mechanistic study of insulin signaling and resistance using human pluripotent stem cell-derived adipocytes.TeaserA new protocol to generate hPSC-adipocytes that respond to physiological insulin levels and can model diabetes.
Evolutionary changes in the chromatin landscape contribute to reorganization of a developmental gene network during rapid life history evolution in sea urchins
Authors: Davidson Phillip L. , Byrne Maria , Wray Gregory A. 
Published: February 2022

ABSTRACTChromatin configuration is highly dynamic during embryonic development in animals, exerting an important point of control in transcriptional regulation. Yet there exists remarkably little information about the role of evolutionary changes in chromatin configuration to the evolution of gene expression and organismal traits. Genome-wide assays of chromatin configuration, coupled with whole-genome alignments, can help address this gap in knowledge in several ways. In this study we present a comparative analysis of regulatory element sequences and accessibility throughout embryogenesis in three sea urchin species with divergent life histories: a lecithotroph Heliocidaris erythrogramma, a closely related planktotroph H. tuberculata, and a distantly related planktotroph Lytechinus variegatus. We identified distinct epigenetic and mutational signatures of evolutionary modifications to the function of putative cis-regulatory elements in H. erythrogramma that have accumulated non-uniformly throughout the genome, suggesting selection, rather than drift, underlies many modifications associated with the derived life history. Specifically, regulatory elements composing the sea urchin developmental gene regulatory network are enriched for signatures of positive selection and accessibility changes which may function to alter binding affinity and access of developmental transcription factors to these sites. Furthermore, regulatory element changes often correlate with divergent expression patterns of genes involved in cell type specification, morphogenesis, and development of other derived traits, suggesting these evolutionary modifications have been consequential for phenotypic evolution in H. erythrogramma. Collectively, our results demonstrate that selective pressures imposed by changes in developmental life history rapidly reshape the cis-regulatory landscape of core developmental genes to generate novel traits and embryonic programs.
Understanding the purchase intentions for organic vegetables across EU: a proposal to extend the TPB model
Authors: Loera Barbara , Murphy Blain , Fedi Angela , Martini Mara , Tecco Nadia , Dean Moira 
Published: February 2022

PurposeThe study aims to propose a systematic and innovative model of purchase intention development that integrates Theory of Planned Behaviour (TPB) with its main extensions and clarifies the logical status of the variables involved and the structure of the causal path.Design/methodology/approachThe TPB is the most useful predictive model of purchase intentions, which can be viewed as the product of various psychological determinants. Previous works have proposed extensions of the TPB model to selectively include knowledge, trust or social norms, but an integrated proposal has not yet been formulated. Based on a survey in four European countries (Germany, Italy, Poland and the UK; N = 1,035), this study tests the process of organic vegetable purchase intention development using a structural equation model (SEM). This comprises part of the measurement of latent variables and part of the analysis of dependency relationships (MLR estimation method).FindingsThe results show that purchase intention for organic vegetables is primarily dependent on positive moral attitude (PoMA) towards such consumption. The inclusion of PoMA reduces the effect of attitude toward buying organic vegetables, but the effects of social norms, past behaviour and perceived behavioural control remain significant. Originality/valueThis study proposes an innovative model to explain purchase intention for organic vegetables that incorporates the key current extensions of the TPB model (knowledge, trust and PoMA) into an integrated causal pathway. Understanding the relationships between the antecedents of purchase intention provides relevant information on “what” needs to be improved and “where” interventions are needed to steer consumers towards organic food.
Local food: a constellation of perceived proximity
Authors: Chicoine Marilyne , Rodier Francine , Durif Fabien 
Published: February 2022

PurposeThe purpose of this study is to explore the definition of local food through the concept of perceived proximity in order to improve the understanding of food locality and to propose a new framework for analysis.Design/methodology/approachThe paper presents an exploratory research through 32 semi-structured interviews with six agri-food industry stakeholders carried out in Quebec, Canada. Thematic analysis is used to identify the main dimensions of the proximity of a local food. A conceptual framework based on the results is presented.FindingsThe results suggest that local food can be defined according to nine dimensions of proximity: geographic, process, price, identity, relational, functional, cultural, access and experiential.Originality/valueThis study allows the concept of local food to be broken down into a constellation of perceived proximities and expands the understanding of the differences in the perception of food locality.
On the application of the GS4-1 framework for fluid dynamics and adaptive time-stepping via a universal A-posteriori error estimator
Authors: Wang Yazhou , Xie Ningning , Yin Likun , Zhang Tong , Zhang Xuelin , Mei Shengwei , Xue Xiaodai , Tamma Kumar 
Published: February 2022

Purpose
The purpose of this paper is to describe a novel universal error estimator and the adaptive time-stepping process in the generalized single-step single-solve (GS4-1) computational framework, applied for the fluid dynamics with illustrations to incompressible Navier–Stokes equations.

Design/methodology/approach
The proposed error estimator is universal and versatile that it works for the entire subsets of the GS4-1 framework, encompassing the nondissipative Crank–Nicolson method, the most dissipative backward differential formula and anything in between. It is new and novel that the cumbersome design work of error estimation for specific time integration algorithms can be avoided. Regarding the numerical implementation, the local error estimation has a compact representation that it is determined by the time derivative variables at four successive time levels and only involves vector operations, which is simple for numerical implementation. Additionally, the adaptive time-stepping is further illustrated by the proposed error estimator and is used to solve the benchmark problems of lid-driven cavity and flow past a cylinder.

Findings
The proposed computational procedure is capable of eliminating the nonphysical oscillations in GS4-1(1,1)/Crank–Nicolson method; being CPU-efficient in both dissipative and nondissipative schemes with better solution accuracy; and detecting the complex physics and hence selecting a suitable time step according to the user-defined error threshold.

Originality/value
To the best of the authors’ knowledge, for the first time, this study applies the general purpose GS4-1 family of time integration algorithms for transient simulations of incompressible Navier–Stokes equations in fluid dynamics with constant and adaptive time steps via a novel and universal error estimator. The proposed computational framework is simple for numerical implementation and the time step selection based on the proposed error estimation is efficient, benefiting to the computational expense for transient simulations.
Renewable energy and CO2 emissions: the economics and geopolitical implications, experiences from the BRICS nations
Authors: Ghosh Sudeshna 
Published: February 2022

Purpose
The purpose of this study is to examine how renewable energy consumption moderates the relationship between inequality and carbon dioxide (CO2) emissions for Brazil, Russia, India, China and South Africa (BRICS). The nexus between energy use and geopolitical tensions has also been explored.

Design/methodology/approach
This study has used distinctive data sets from 1990 to 2018 to explore the interconnections on emission, energy use, inequality and geopolitics. To do away with the difficulties related to heterogeneity and cross-sectional dependence (CD), this paper uses recent estimation methods that are robust to panel heterogeneity and CD.

Findings
The results of the panel augmented mean group (AMG) estimation and common correlated effects mean group (CCEMG) estimation verify the environmental Kuznets curve. The findings show that a 1% rise in Gini inequality leads to a 0.24% rise in the CO2 emission (AMG) method and a 0.17% rise in emissions CCEMG (method). As far as the moderating impact of renewable energy upon Gini measure of inequality is concerned, it is −0.10 AMG and CCEMG methods of estimation, respectively. However, the moderating impact of renewable energy on the geopolitical index leads to a mitigating impact on CO2 emissions, 0.55% decline in AMG method.

Originality/value
This research makes a distinctive contribution by investigating for the first time to the best of the authors’ knowledge the main pillars of sustainable ecological development in the context of the BRICS nations.
Employing machine learning for capturing COVID-19 consumer sentiments from six countries: a methodological illustration
Authors: Schlegelmilch Bodo B. , Sharma Kirti , Garg Sambbhav 
Published: February 2022

PurposeThis paper aims to illustrate the scope and challenges of using computer-aided content analysis in international marketing with the aim to capture consumer sentiments about COVID-19 from multi-lingual tweets.Design/methodology/approachThe study is based on some 35 million original COVID-19-related tweets. The study methodology illustrates the use of supervised machine learning and artificial neural network techniques to conduct extensive information extraction.FindingsThe authors identified more than two million tweets from six countries and categorized them into PESTEL (i.e. Political, Economic, Social, Technological, Environmental and Legal) dimensions. The extracted consumer sentiments and associated emotions show substantial differences across countries. Our analyses highlight opportunities and challenges inherent in using multi-lingual online sentiment analysis in international marketing. Based on these insights, several future research directions are proposed.Originality/valueFirst, the authors contribute to methodology development in international marketing by providing a “use-case” for computer-aided text mining in a multi-lingual context. Second, the authors add to the knowledge on differences in COVID-19-related consumer sentiments in different countries. Third, the authors provide avenues for future research on the analysis of unstructured multi-media posts.
Strengths and shortcomings of Latin American participation in post-conflict Colombia
Authors: Hirst Monica E. , Dalponte Bruno 
Published: February 2022

Purpose
The purpose of this paper is to analyse the characteristics of the Latin American participation in the United Nations Mission in Colombia (UNMC), looking into the contributions of regional actors to the peace process in the immediate post-conflict. Testimonies from Latin American actors involved in the demobilization, disarmament and reintegration process help identify their perceptions of strengths and shortcomings of the mission. This paper analyses both the enduring relevance of their individual/institutional trajectories and the adaptations needed to implement the Peace Agreement in the Colombian context.

Design/methodology/approach
This is an exploratory case study. Primary data collection relied on in-depth, semi-structured interviews, allowing the authors to tap into how the trajectories of those involved help explain continuities and innovations of the UNMC regarding previous UN missions.

Findings
This paper argues that cultural factors are central to understand how Latin Americans participated in the UNMC. The design of the Monitoring and Verification Mechanism and the inclusion of a strong gender agenda make the UNMC a salient case, both for scholars studying Latin American presence in peace processes and for those looking into peace building, more generally.

Originality/value
The fieldwork, conducted with military/security forces, UN officials and civilians, has made available a diverse spectrum of testimonies that provide crucial insights into “lessons learned”, contributing to tracing the trajectories of these actors and providing insights for the improvement of future political and peacebuilding multilateral missions.
How ethnic dissimilarity influences perceived organizational support and organizational citizenship behaviors?
Authors: Tan Luen Peng , Choong Yuen Onn , Yap Ching Seng , Choe Kum Lung , Rungruang Parisa , Li Zhen 
Published: February 2022

PurposeThis study examines the mediating effect of self-efficacy between perceived organizational support (POS) and organizational citizenship behaviors (OCB) organization (OCBO); and POS and OCB individual (OCBI) in a cross-cultural context.Design/methodology/approachData were collected from 207 full-time academics from the private universities in Malaysia and Mainland China using a questionnaire survey.FindingsThe results indicate that self-efficacy is a salient mediator linking POS with OCBI and OCBO. Additionally, ethnic dissimilarity is found to have a contextual influence on the research model as the results reveal that self-efficacy only mediates the relationship between POS and OCBO but not between POS and OCBI in a heterogeneous society. In contrast, self-efficacy is found to mediate the relationships between POS and OCBO and between POS and OCBI in a homogeneous society.Originality/valueThis study contributes to the literature by being one of the first studies that examine the relationship between self-efficacy and two dimensions of OCB in two different cultural contexts.
The need for new public policies to increase entrepreneurship and spur economic growth
Authors: Singh Robert P. 
Published: February 2022

PurposeThe purpose of this viewpoint paper is to bring attention to the declining rate of entrepreneurship, its effect on the broader economy, the need for public policy solutions and to offer several such solutions. Government intervention is needed to address the stagnation trends discussed throughout the paper. It is up to academic scholars, researchers and think tanks to make government leaders aware of the trends and possible solutions.Design/methodology/approachA literature review was performed and the need to increase entrepreneurship through public policies was discussed.FindingsThe current ten-year economic expansion is suffering from secular stagnation as a result of diminishing new venture creation and slower adoption of technological innovations. Economic policies are heavily tilted toward large, established firms even as new entrepreneurial ventures have greater impact on job creation. The importance of using public policies to spur economic growth through greater opportunity-based entrepreneurship is discussed. Unique tax policy changes are suggested as well as a proposed national business plan competition.Originality/valueThis paper makes a contribution to the entrepreneurship and public policy literature by discussing how the declining rate of entrepreneurship and slower adoption of new technology are related to the relatively tepid pace of economic expansion seen over the last decade in the US. The proposed policies focus on increasing new ventures based on opportunity-based entrepreneurship, and it is hoped that this will help in the development of other policies and spur new lines of research and knowledge that lead to increased economic growth.