bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efb released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3
Authors: Zhang Xiaokai , Xiong Tingrong , Gao Lin , Wang Yu , Liu Luxuan , Tian Tian , Shi Yun , Jinyong Zhang , Zhao Zhuo , Lu Dongshui , Luo Ping , Cheng Ping , Zhang Weijun , Jing Haiming , Gou Qiang , Zeng Hao , Yan Dapeng , Zou Quanming 
Published: February 2022

Abstract
Many pathogens secrete effectors to hijack intracellular signaling regulators in host immune cells to promote pathogenesis. However, the pathogenesis of Staphylococcus aureus (S. aureus) secretory effectors within host cells is unclear. Here, we report that intracellular S. aureus secretes fibrinogen binding protein (Efb) into the cytoplasm of macrophages to inhibit proinflammatory cytokine expression and suppress host immunity. Mechanistically, RING finger protein 114, a host E3 ligase, mediates K27-linked ubiquitination of Efb at lysine 71, which facilitates the recruitment of TNF receptor associated factor 3 (TRAF3). The binding of Efb to TRAF3 disrupts the formation of the TRAF3/TRAF2/cIAP1 complex, which mediates K48-ubiquitination of TRAF3 to promote degradation, resulting in suppression of the inflammatory signaling cascade. Additionally, the Efb K71R mutant loses the ability to inhibit inflammation and exhibits decreased pathogenicity. Therefore, our findings identify an unrecognized mechanism of S. aureus to suppress host defense, which may be a promising target for developing effective anti-S. aureus immunomodulators.
Lean, Agile, Resilient, And Green Human Resource Management: The Impact On Organizational Innovation And Organizational Performance
Authors: Alipour Nima , Nazari-Shirkouhi Salman , Sangari Mohamad Sadegh , Vandchali Hadi Rezaei 
Published: February 2022

Abstract
There are four paradigms of lean, agile, resilient, and green (LARG) which can promote human resource culture to create novel ideas and increase performance in organizations. This study aims to conceptualize lean, agile, resilient, and green paradigms in human resource management (HRM) and investigates how different LARG HRM elements can affect organizational innovation and performance. In this way, a conceptual model for investigating the LARG concept in HRM is proposed. A new tool to measure lean, agile, resilient, and green indicators in service industry has been developed. Using convenience sampling method to access possible respondents, a survey questionnaire is managed to collect data from 102 service sector organizations in Iran. The collected data are analyzed by partial least squares-structural equation modeling (PLS-SEM). The results indicate that the LARG HRM significantly and positively influences organizational performance. In addition, the LARG HRM indirectly affects organizational performance through organizational innovation. This study provides valuable insights into implementing the LARG HRM in organizations and how it contributes to enhancing organizational performance.
Health Outcomes, Health Services Utilization, and Costs Consequences of Medicare Uninsurance among Migrants in Canada: A Systematic Review
Authors: Garasia Sophiya , Bishop Valerie , Clayton Stephanie , Pinnington Genevieve , Arinze Chika , Jalil Ezza 
Published: February 2022

Abstract
Background: Medically uninsured groups, many of them migrants, reportedly delay using healthcare services due to costs and often face preventable health consequences. This systematic review sought to assess quantitative evidence on health outcomes, health care utilization, and health care costs among uninsured migrant populations in Canada.Methods: OVID MEDLINE, Embase, Global Health, EconLit, and grey literature were searched to identify relevant literature published up until March 2021. The Cochrane Risk of Bias in Non-randomized Studies – of Interventions (ROBINS-I) tool was used to assess the quality of studies.Results: 10 studies were included. Data showed there are differences among insured and uninsured groups in reported health outcomes and healthcare use. No quantitative studies on economic costs were captured. Conclusions: Our findings indicate a need to review policies regarding accessible and affordable health care for migrants. Increasing funding to community health centers may improve service utilization and health outcomes among this population.
Proteomic signature of tubulointerstitial tissue predicts prognosis in IgAN
Authors: Paunas Flavia Teodora Ioana , Finne Kenneth , Leh Sabine , Marti Hans-Peter , Berven Frode , Vikse Bjørn Egil 
Published: February 2022

Abstract
Background: IgA nephropathy (IgAN) is associated with a significant risk of progression to kidney failure. Tubular atrophy is an established important risk factor for progressive disease, but few studies have investigated tubulointerstitial molecular markers and mechanisms of progression in IgAN. Methods: Based on data from the Norwegian Renal Registry, two groups were included: IgAN patients with (n=9) or without (n=18) progression to kidney failure during 10 years of follow-up. Tubulointerstitial tissue without discernible interstitial expansion or pronounced tubular alterations was microdissected, proteome was analysed using tandem mass spectrometry and relative protein abundances were compared between groups. Results: Proteome analyses quantified 2562 proteins with at least 2 unique peptides. Of these, 150 proteins had significantly different abundance between progressive and non-progressive IgAN patients, 67 were more abundant and 83 less abundant. Periostin was the protein with the highest fold change between progressive and non-progressive IgAN (fold change 8.75, p<0.05) and periostin staining was also stronger in patients with progressive vs non-progressive IgAN. Reactome pathway analyses showed that proteins related to inflammation were more abundant and proteins involved in mitochondrial translation were significantly less abundant in progressive vs non-progressive patients.Conclusions: Microdissection of tubulointerstitial tissue with only mild damage allowed for identification of proteome markers of early progressive IgAN. Periostin abundance showed promise as a novel and important risk marker of progression.
Trends and Forecasts on Prediabetes and Diabetes in Adult and Elderly Population in Turkey
Authors: Satman Ilhan , Bayirlioglu Safak , Okumus Funda , Erturk Nazli , Yemenici Merve , Cinemre Sedanur , Gulfidan Gizem , Arga Kazim Yalcin , Merih Yeliz Dogan , Issever Halim 
Published: February 2022

Abstract
Aims Diabetes is a chronic disease that limits the quality and duration of life. We aimed to estimate the trends in prediabetes and diabetes between 2010-2020, and the projections to 2023 and 2045 in Turkey. Materials and methods Prediabetes and diabetes estimates were calculated by direct standardization using age/sex-specific data from the previous TURDEP-II survey as reference. The 2010-2020 population demographics were obtained from TurkStat. Comparative age-adjusted diabetes rates were estimated using standard population models of world and Europe. ResultsEstimates depicted that the population (20-84 years) of any degree of glucose intolerance in Turkey increased by approximately 5.3 million (diabetes: 2.2 million and prediabetes: 3.1 million) from 2010 to 2020. While the increase in prediabetes and diabetes rates was 22% and 31.6% in overall population, corresponding increase were 45.2% and 45.6% in the elderly. Notably, diabetes awareness was comparable (54% and 58%). Age-specific prediabetes and diabetes rates were significantly higher in women than in men (diabetes: 14.1% versus 17%; prediabetes: 38.6% versus 30.2%). The comparative age-adjusted prevalence of diabetes to the standard European but not the world population model, showed a higher rate of 19.4%. According to the projections the prevalence of diabetes will reach 15.9% in 2023 and 19.1% in 2045. Conclusion﻿Diabetes has reached epidemic proportions in Turkey and will continue to grow in the coming decades. The fact suggests that there is an urgent need to developing and implementing the country-specific prevention program to halt this increase. Otherwise, the burden of diabetes on social, economic and health services will continue to increase.
Minimal non-abelian nodal braiding in ideal metamaterials
Authors: Qiu Chunyin , Qiu Huahui , Zhang Qicheng , Liu Tingzhi , Fan Xiying , Zhang Fan 
Published: February 2022

Abstract
Exploring new topological phases and phenomena has become a vital topic in condensed matter physics and material sciences1-4. It is generally believed that a pair of band nodes with opposite topological charges will annihilate after collision. Recent studies reveal that a braided colliding nodal pair can be stabilized in a multi-gap system with PT or C_2z T symmetry5-7. Beyond the conventional single-gap abelian band topology, this intriguing phenomenon exemplifies non-abelian topological charges5-7. Here, we construct ideal acoustic metamaterials to realize non-abelian braiding with the fewest band nodes. We experimentally observe an elegant but nontrivial nodal braiding process, including nodes creation, braiding, collision, and repulsion (i.e., failure to annihilate), and measure the mirror eigenvalues to elucidate the essential braiding consequence. The latter, at the level of wavefunctions, is of prime importance since the braiding physics essentially aims to entangle multi-band wavefunctions. Furthermore, we experimentally unveil the highly intricate correspondence between the edge responses and the bulk non-abelian charges. Notably, all our experimental data perfectly reproduce our numerical simulations. Our findings pave the way for developing non-abelian topological physics that is still in its infancy8-10.
Efficient Cloud-based Resource Sharing Through Multi-tenancy and Load Balancing: An Exploration of Higher Education and Digital Libraries
Authors: Al-Maliki Saeed Q. Al-Khalidi 
Published: February 2022

Abstract
In recent years, cloud computing has received a lot of attention in academic and industrial sectors. Cloud technology facilitates data sharing and enables cost efficiency, playing a vital role in the present as well as for the foreseeable future. In this paper, we briefly elaborate on the structure of cloud-based digital resource sharing suitable for academic and digital libraries by implementing multi-tenant and load-balancing techniques. We then suggest improving the current user service model with private cloud storage as a new paradigm for digital resource sharing for other sectors, including medical and financial. This paper shows a summary of cloud computing and its possible applications, combined with digital data optimisation for web-based services. This study could aid in the identification and generation of cloud-based services that use multi-tenant and load-balancing methodologies for the best quality of service.
A hedonic orexigenic subnetwork within the human hippocampus
Authors: Barbosa Daniel A. N. , Gattas Sandra , Salgado Juliana S. , Kuijper Fiene Marie , Wang Allan R. , Huang Yuhao , Kakusa Bina , Leuze Christoph , Luczak Artur , Rapp Paul , Malenka Robert C. , Miller Kai J. , Heifets Boris D. , Bohon Cara , McNab Jennifer A. , Halpern Casey H. 
Published: February 2022

Abstract
Only recently has the rodent hippocampus been implicated in orexigenic appetitive processing1,2. This function has been found to be mediated at least in part by lateral hypothalamic inputs involving an orexigenic neuropeptide, melanin-concentrating hormone3. This circuit remains elusive in humans. Here, we combine tractography, intracranial electrophysiology, cortico-subcortical evoked potentials, and brain-clearing 3D histology to identify an orexigenic circuit involving the lateral hypothalamus converging in a hippocampal subregion. We found that low-frequency power is modulated by sweet-fat food cues and this modulation was specific to the dorsolateral hippocampus. Lastly, structural and functional analyses of this circuit in a human cohort exhibiting dysregulated eating behavior revealed connectivity that was inversely related to body mass index. Collectively, this multimodal approach describes an orexigenic subnetwork within the human hippocampus implicated in obesity and related eating disorders.
Diagnostic Performance of Endoscopic Ultrasound Elastography for Differential Diagnosis of Solid Pancreatic Lesions: A Propensity Score-Matched Analysis
Authors: Cho In Rae , Jeong Seok-Hoo , Kang Huapyong , Kim Eui Joo , Kim Yeon Suk , Jeon Soyoung , Cho Jae Hee 
Published: February 2022

Abstract

Background
Endoscopic ultrasound-elastography (EUS-EG) is a non-invasive complementary diagnostic method for the differential diagnosis of solid pancreatic lesions (SPL). However, the optimal strain ratio (SR) value and diagnostic performance of EUS-EG have not yet been determined in pancreatic neuroendocrine neoplasm (PNEN), mass-forming pancreatitis (MFP), and pancreatic ductal adenocarcinoma (PDAC). We aimed to determine the optimal SR value in EUS-EG for differential diagnosis of SPLs.
Methods
A total 134 patients who underwent EUS-EG to evaluate SPL were retrospectively investigated. Patients were divided into 3 groups – PNEN (n=10), MFP (n=37), and PDAC (n=87). Baseline characteristics and results of EUS-EG were compared between groups and statistical analyses were performed for differential diagnosis.
Results
The mean SR values of each group were 11.85±7.56 (PNEN), 11.45±5.97 (MFP), and 22.50 ± 13.19 (PDAC), respectively. There was significant association between SR value increase and PDAC diagnosis (PNEN versus PDAC, p=0.0216; MFP versus PDAC, p=0.0006) in multinomial logistic regression analysis. SR value of 17.14 was confirmed as the optimal cut-off value for differential diagnosis between MFP and PDAC.
Conclusions
Differential diagnosis of SPL could be performed using EUS-EG. The cut-off SR value presented in this study is expected to be useful in differential diagnosis.
Clinical trial registration number:
https://cris.nih.go.kr/cris : KCT0002082
Biomimetic Cardiac Tissue Culture Model (CTCM) to Emulate Cardiac Physiology and Pathophysiology ex vivo
Authors: Miller Jessica , Meki Moustafa , Ou Qinghui , Abouleisa Riham , Tang Xian-Liang , Salama Abou Bakr , Gebreil Ahmad , Lin Cndy , Abdeltawab Hisham , Khalifa Fahmi , Hill Bradford , Abi-Gerges Najah , Bolli Roberto , El-Baz Ayman , Giridharan Guruprasad , Mohamed Tamer 
Published: February 2022

Abstract
Background: There is an urgent need for a reliable in vitro system that can accurately replicate the cardiac physiological environment for drug testing. The limited availability of human heart tissue culture systems has led to inaccurate interpretations of cardiac related drug effects. Our group had previously developed a culture system for pig/human heart slices that enables functional and structural viability for 6 days under electric stimulation and enriched media and can reliably demonstrate the subacute toxic effects of anti-cancer therapeutics. However, by day 10, the slices undergo cardiomyocyte dedifferentiation and fibrotic remodeling, making them inadequate for long-term drug testing.Aims: To determine whether including physiological mechanical and humoral cues within the culture system can prolong the viability of the heart tissue in culture.Methods and Results: We have developed a novel cardiac tissue culture model (CTCM) that can electro-mechanically stimulate heart slices with physiological preload and afterload during each cardiac cycle. After 12 days in culture, this approach was able to partially improve the viability of heart slices, but not to completely maintain their structural integrity. Therefore, following small molecule screening, we found that incorporation of 100 nM tri-iodo-thyronine (T3) and 1 μM dexamethasone (Dex) into our culture media preserved microscopic structure of the slices for 12 days. When combined with T3/Dex treatment, the CTCM system was able to maintain the transcriptional profile, viability, metabolic activity, and structure integrity for 12 days at the same levels as the fresh heart tissue. Furthermore, overstretching the cardiac tissue induced cardiac hypertrophy after 6 days in culture, which provides the first proof of concept for the ability of the CTCM to emulate cardiac pathophysiology. Conclusions: This manuscript describes a new physiological culture system for heart slices that incorporates physiological mechanical and humoral cues for maintaining viability and functionality of the heart slices. The CTCM has the potential to emulate cardiac physiology and pathophysiology in culture for extended period of time, thereby enabling reliable drug screening.
Association of Periprocedural Phentolamine Infusion with Favorable Outcome in Patients with Chronic Kidney Disease and Chronic Coronary Syndrome undergoing coronary catheterization: A Prospective Randomized Controlled Study
Authors: Hamila Mohamed Abo , Ghawaby Helmy El , Zaki Mohamed , Soliman Mohamed , Gabr Khaled 
Published: February 2022

Abstract
Background: Chronic kidney disease (CKD) is a major risk factor for contrast induced nephropathy acute kidney injury (CIN-AKI) in chronic coronary syndrome (CCS) patients undergoing coronary catheterization. We aimed to evaluate the efficacy of phentolamine in prevention of CIN-AKI in CKD and CCS patients undergoing percutaneous coronary catheterization for diagnostic angiography ± stenting.Methods: Participants with CKD and CCS planned for percutaneous coronary catheterization were included, while participants with normal kidney functions were excluded. A consecutive sample of 107 participants (mean age 58.62 ± 8.96 years, 64.5% males) was selected, underwent diagnostic coronary angiography or percutaneous coronary intervention, and received either conventional CIN-AKI prevention strategy (group 1) or periprocedural phentolamine and conventional CIN-AKI prevention strategy (group 2).Results: The percentages of study participants who had CIN-AKI were 82.9% for group 1 and 17.1% for group 2, respectively. There were a statistically significant association between periprocedural phentolamine and prevention of CIN-AKI (OR = 0.041, 95% CI 0.0149 – 0.1128, P <0.0001) and statistically significant differences between group 1 versus group 2 regarding the urine output (ml/kg) and the urine output (ml/hour) within 72 hours post procedure, the peak change in serum creatinine within 72 hours post procedure, the percentage of change relative to the baseline serum creatinine at 72 hours post procedure, and the incidence rate of major adverse cardiac and cerebrovascular events within 90 days post procedure (t(105) = -0.69, p < 0.001, t(105) = -52.46, p < 0.001, t(102) = 0.2, p 0.018, t(102) = 23.54, p < 0.001, and t(102) = 1.168, P <0.001, respectively).Conclusions: The incidence of CIN-AKI and the peak change in serum creatinine within 72 hours post procedure was lower in CKD patients who underwent coronary catherization and received phentolamine infusion versus intravenous saline. The significant association of phentolamine infusion with prevention of CIN-AKI highlights the potential role of phentolamine for protection of the kidney in CKD patients planned for coronary catherization.Trial Registration Number: I-111015
Visualized analysis of the trend and hotspots in microbial spatial ecology: a bibliometric study from 2000 to 2020
Authors: Hussain Sarfraz , Liu Hao , Sabir Rabia , Khan Muzammil Hassan , Cao Hui 
Published: February 2022

Abstract
Spatial ecology is a broad-spectrum term used in a variety of studies depending on the subdiscipline of life. Spatial ecological studies focus on patterns and interactions within and between species at the local, regional and global scales. The scientific literature published on spatial microbial ecology is ample and covers a wide array of objectives, including biogeographic patterns of microorganisms, international collaboration, hot topics, keywords and productive journals. We used a science mapping approach to provide a global and systematic bibliometric synthesis in this area. A total of 7022 publications on spatial microbial ecology were retrieved from the Web of Science database in 2000–2020. Temporal analyses showed a rapid growth in publications and citations noted after 2010. Results based on cluster analysis revealed that the top productive countries in this area belong to developed countries other than China. Keyword analysis showed that diversity, bacteria and dynamics are research hotspots in this field. The citation burst indicated that researchers focused on traditional techniques in the early stage, while new techniques and models were developed in the later stage. These findings suggest that spatial microbial ecology is still in a developmental stage. This study provides a systematic understanding of the development and panoramic road map for conducting future research in spatial microbial ecology.
Establishment of a Novel Mouse Model of Adenomyosis Suitable for Longitudinal and Quantitative Analysis and Perinatal Outcome Studies
Authors: Elsherbini Mohammed , Koga Kaori , Hiraoka Takehiro , Kumasawa Keiichi , Satake Erina , Taguchi Ayumi , Makabe Tomoko , Takeuchi Arisa , Izumi Gentaro , Takamura Masashi , Harada Miyuki , Hirata Tetsuya , Hirota Yasushi , Wada-Hiraike Osamu , Osuga Yutaka 
Published: February 2022

Abstract
Background: The purpose of this study was to establish a novel mouse model of adenomyosis suitable for longitudinal and quantitative analyses and perinatal outcome studies. Methods: Using a 30 G needle, the entire uterine wall of one horn was mechanically punctured at a frequency of 100 times/1 cm (adenomyosis horn). The other horn was left unpunctured (control horn). Mice were sacrificed on day 14 (D14) or day 65 (D65). The uterus was fixed, paraffin-embedded, sliced, and stained. Lesions were detected and counted, and their volumes were measured. Cell proliferation and fibrosis were assessed by Ki67 and Masson’s Trichrome staining, respectively. Blood vessels were detected using CD31 immunostaining. Some of the mice were mated, and the date of delivery, litter size, number of implantations, and number and volume of postpartum lesions were measured. Results: The number of lesions per horn did not differ between D14 and D65. The volume of the entire lesion was significantly greater on D65 than on D14 (p < 0.0001). The volume of the epithelial part of the lesion was significantly greater in D65 (p< 0.0001). The volume of the stromal part of the lesion was also greater on D65 (p < 0.0001). The percentage of Ki67 positive cells in the epithelial part of the lesion was significantly higher on D14 (p < 0.05). In contrast, the percentage of Ki67-positive cells in the stromal part was significantly higher on D65 (p < 0.01). Vascular density in the lesions was higher in on D65 (p < 0.05). The percentage of fibrotic area was significantly higher on D65 (p < 0.01). The date of delivery was slightly earlier than that reported for healthy mice of the same strain. The litter size was smaller than that reported in previous research. The number of implantation sites did not differ between the control and the adenomyosis horn. The number and volume of lesions did not differ between the non-pregnant and postpartum groups. Conclusions: This model can be applied to evaluate the pathogenesis of adenomyosis, validate the efficacy of therapeutic agents, and evaluate the effect of adenomyosis on pregnancy and vice versa.