bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined roles of exporters in acetic acid tolerance in Saccharomyces cerevisiae
Authors: Zhang Xiaohuan , Nijland Jeroen G. , Driessen Arnold J. M. 
Published: June 2022

AbstractAcetic acid is a growth inhibitor generated during alcoholic fermentation and pretreatment of lignocellulosic biomass, a major feedstock to produce bioethanol. An understanding of the acetic acid tolerance mechanisms is pivotal for the industrial production of bioethanol. One of the mechanisms for acetic acid tolerance is transporter-mediated secretion where individual transporters have been implicated. Here, we deleted the transporters Aqr1, Tpo2, and Tpo3, in various combinations, to investigate their combined role in acetic acid tolerance. Single transporter deletions did not impact the tolerance at mild acetic acid stress (20 mM), but at severe stress (50 mM) growth was decreased or impaired. Tpo2 plays a crucial role in acetic acid tolerance, while the AQR1 deletion has a least effect on growth and acetate efflux. Deletion of both Tpo2 and Tpo3 enhanced the severe growth defects at 20 mM acetic acid concomitantly with a reduced rate of acetate secretion, while TPO2 and/or TPO3 overexpression in ∆tpo2∆tpo3∆ restored the tolerance. In the deletion strains, the acetate derived from sugar metabolism accumulated intracellularly, while gene transcription analysis suggests that under these conditions, ethanol metabolism is activated while acetic acid production is reduced. The data demonstrate that Tpo2 and Tpo3 together fulfill an important role in acetate efflux and the acetic acid response.
Defining minimal detectable difference in echocardiographic measures of right ventricular function in systemic sclerosis
Authors: Mukherjee Monica , Mercurio Valentina , Balasubramanian Aparna , Shah Ami A. , Hsu Steven , Simpson Catherine E. , Damico Rachel , Kolb Todd M. , Hassoun Paul M. , Mathai Stephen C. 
Published: June 2022

Abstract
Background
Echocardiography (2DE) is integral for screening and longitudinal evaluation of pulmonary arterial hypertension (PAH) in systemic sclerosis (SSc). In the present study, we sought to establish the reliability, repeatability, and reproducibility of 2DE parameters in SSc patients with and without PAH and to define the minimal detectable difference (MDD), the smallest change detected beyond measurement error.

Methods
SSc patients without known PAH and with invasively confirmed PAH on stable therapies underwent 2DE with strain at two time points. Analysis of variance (ANOVA) and coefficients of variation (CV) were calculated to assess for repeatability, reliability, and reproducibility. Intra- and inter-observer agreement were assessed using intraclass correlation. Bland-Altman analysis explored the level of agreement between evaluations. MDD was calculated using the standard error of measurement for each parameter by cohort.

Results
ANOVA demonstrated few significant differences between evaluations across groups. Global right ventricular longitudinal systolic strain (GRVLSS, 9.7%) and fractional area change (FAC, 21.3%) had the largest CV, while tricuspid annular plane excursion (TAPSE), S’ wave, and right ventricular outflow track velocity time integral (RVOT VTI) were 0.87%, 3.2%, and 6.0%, respectively. Intra- and inter-observer agreement was excellent. MDD for TAPSE, FAC, S’ wave, RVOT VTI, GRVLSS, and RVSP were 0.11 cm, 0.03%, 1.27 cm/s, 0.81 cm, 1.14%, and 6.5 mmHg, respectively.

Conclusions
We demonstrate minimal measurement error in clinically important 2DE-based measures in SSc patients with and without PAH. Defining the MDD in this population has important implications for PAH screening, assessment of therapeutic response, and sample size calculations for future clinical trials.
New-onset axillary lymphangioma: a case report
Authors: Kurumety Sasha , Morris Michael , Aydi Zeynep Bostanci 
Published: June 2022

AbstractBackgroundLymphangioma is a rare diagnosis in adult patients and typically presents in early infancy. These tumors are a result of malformation of the lymphatic vessels and usually involve the head, neck, and axilla.Case presentationWe report the case of a 28-year-old African female who recently immigrated from East Africa and presented to our surgical breast clinic with a large and rapidly growing left axillary mass. Initial history and evaluation were concerning for hydatid cyst; however, on surgical excision, gross appearance was consistent with cystic lymphangioma. Diagnosis was confirmed on pathology review.ConclusionsAlthough lymphangiomas are typically found in young children, adults may develop these tumors in response to unknown triggers. Surgical excision is the preferred treatment.
Microbial succession and exploration of higher alcohols-producing core bacteria in northern Huangjiu fermentation
Authors: Yan Yi , Sun Leping , Xing Xuan , Wu Huijun , Lu Xin , Zhang Wei , Xu Jialiang , Ren Qing 
Published: June 2022

AbstractHigher alcohols (HAs) are abundant compounds that provide important flavors in Huangjiu, but they also cause hangover. Previous studies have shown the production of HAs to be related to yeast, but the correlations between HAs and other microorganisms are rarely reported. In this study, we detected changes in levels of HAs and microbial dynamics during the Huangjiu fermentation process. Relationships were characterized using Pearson’s correlation coefficient. The functional core HA-producing bacteria were selected by bidirectional orthogonal partial least squares (O2PLS). The result showed that 2-methyl-1-propanol, phenethyl alcohol and 3-methyl-1-butanol were the principle HAs present at high levels. Lactococcus and Saccharomyces were predominant at the genus level of bacteria and fungi, respectively. A total of 684 correlations between HAs and microorganisms were established. Five genera were screened as functional core HA-producing bacteria. Our findings might provide some new inspiration for controlling the content of HAs, enhancing international prestige and market expansion of Huangjiu.
Adaptive recovery of dictionary-sparse signals using binary measurements
Authors: Beheshti Hossein , Daei Sajad , Haddadi Farzan 
Published: June 2022

AbstractOne-bit compressive sensing (CS) is an advanced version of sparse recovery in which the sparse signal of interest can be recovered from extremely quantized measurements. Namely, only the sign of each measure is available to us. The ground-truth signal is not sparse in many applications yet can be represented in a redundant dictionary. A strong line of research has addressed conventional CS in this signal model, including its extension to one-bit measurements. However, one-bit CS suffers from an extremely large number of required measurements to achieve a predefined reconstruction error level. A common alternative to resolve this issue is to exploit adaptive schemes. We utilize an adaptive sampling strategy to recover dictionary-sparse signals from binary measurements in this work. A multi-dimensional threshold is proposed for this task to incorporate the previous signal estimates into the current sampling procedure. This strategy substantially reduces the required number of measurements for exact recovery. We show that the proposed algorithm considerably outperforms the state-of-the-art approaches through rigorous and numerical analysis.
An innovative document image binarization approach driven by the non-local p-Laplacian
Authors: Ait Bella Fatim Zahra , El Rhabi Mohammed , Hakim Abdelilah , Laghrib Amine 
Published: June 2022

AbstractText image binarization is fairly a tedious task and a significant problem in document image analysis. This process, as a necessary pretreatment for noisy images with stains, non-uniform background, or degraded text characters, can successfully improve the quality of the image and facilitate the subsequent image processing steps. A theoretically well-motivated non-local method for document image binarization is addressed in this paper. This approach enhances degraded images by estimating and then removing the undesirable background. Extensive experiments conducted on degraded document images evince the greater effectiveness of the proposed non-local algorithm.
Adaptation and validation of the Online-Fear of Missing Out Inventory into Turkish and the association with social media addiction, smartphone addiction, and life satisfaction
Authors: Bakioğlu Fuad , Deniz Metin , Griffiths Mark D. , Pakpour Amir H. 
Published: June 2022

Abstract
Background
In online environments, fear of missing out (FoMO) is where individuals become constantly preoccupied with what others are doing online and feel unable to log off in case they miss something. FoMO is a concept associated with the use of online social media (OSM; e.g., Facebook use, Instagram use) and various scales have been developed to assess the concept. One such scale is the Online Fear of Missing Out (On-FoMO) Inventory. The present study translated the On-FoMO Inventory into Turkish and its main aim was to test the validity and reliability of the scale. The secondary aim was to investigate the relationships between FoMO, social media addiction, smartphone addiction, and life satisfaction.

Methods
A total of 419 participants (289 females and 130 males, mean age = 25.43 years, SD = 6.37) completed a self-report questionnaire including the On-FoMO Inventory, Fear of Missing Out Scale, Bergen Social Media Addiction Scale, Smartphone Addiction Scale-Short Version, and Satisfaction with Life Scale. In the adaptation process of the On-FoMO Inventory, confirmatory factor analysis, concurrent validity, and reliability analyses were performed.

Results
The four-factor structure of the On-FoMO Inventory was confirmed and the Turkish version of the scale demonstrated good reliability. Online FoMO was positively related to social media addiction and smartphone addiction, and negatively related to life satisfaction.

Conclusion
The results showed that the Turkish version of the On-FoMO Inventory has strong psychometric properties.
Development of a smart tourism integration model to preserve the cultural heritage of ancient villages in Northern Guangxi
Authors: Li Wen Zhen , Zhong Hong 
Published: June 2022

Abstract
The modern rural areas represent a vulnerable category that is in special need of sustainable development these days. However, the practice shows that, in the twenty-first century, this sustainability can be assured through the construction of smart villages. The ultimate goal of this study was to create a model for the development of ancient Guangxi villages that will take into account the cultural and tourist dimensions and will be based on the Smart Village concept. Analysis of existing approaches to the implementation of the Smart Village initiatives as well as of regional and local regulatory documents in force allowed identifying key aspects (factors) in this respect. Local government officials, service providers, and local residents were surveyed, and the sample was 586 people. They were asked to rate the implementation in their village of key aspects of the Smart Village concept from 1 to 5 on a Likert scale (from "very bad" to "excellent"). According to the assessments collected, the top-rated and, accordingly, the most developed, was the Technological factor group, followed by the groups encompassing Culture and Tourism, then Economic, then Environmental, and Social factors. These data provided a good foundation for building a Smart Village-based model for the development of six ancient Guangxi villages, known for their unique cultural heritage (Xingping, Daxu, Huangyao, Jixiang, Fuli, and Longji Zhuang). In the future, it can be taken advantage of by government representatives and managers in respect to rural areas with conditions similar to those discussed within the limits of the current study (with certain regional adjustments).
Debriefing strategies for interprofessional simulation—a qualitative study
Authors: Holmes Catherine , Mellanby Edward 
Published: June 2022

Abstract
Background
Interprofessional education is becoming more common worldwide. Simulation is one format in which this can effectively take place. The debriefing after the simulation is a critical part of the simulation process as it allows reflection and discussion of concepts that arose during the simulation. Debriefing has been noted to be challenging in the literature. Debriefing after interprofessional simulation (IPS) is likely to have even more challenges, many related to the different backgrounds (profession, specialty) of the learners. This study was designed to investigate: ‘How do differing learner professions impact on delivery of post simulation debriefing after team based interprofessional simulation—what are the challenges and what strategies can be used to overcome them?’

Methods
An initial review of the literature was used to identify current understanding and potential themes requiring further exploration. Using the results from the literature as a starting point for topics and questions to be asked, semi-structured interviews were planned, with those who are experienced in debriefing after IPS. The interviews were transcribed then analysed using a framework analysis.

Results
The literature search resulted in twenty relevant papers. Four dimensions were drawn out from these papers that were directly related to debriefing after IPS: ‘the debriefer’, ‘method of debriefing’, ‘the learner’ and ‘psychological safety’. Sixteen interviews occurred between June and August 2020. Ten themes were extracted from the analysis of the transcripts of these interviews: number and specialty of debriefers, credibility, assumptions/preconceptions, nurses vs doctors, method of debriefing, the learner, hierarchy, safe learning environment, inclusion of all learners, and number of debriefers. These themes were fitted in the four dimensions identified in the literature search, and discussed as so.

Conclusion
Several challenges and strategies were identified during this study. ‘It depends’ was a common answer received in the interviews suggesting that there is very little advice that can be given that applies to every situation. The main recommendation from this study is the support for an interprofessional group of debriefers in IPS although this does introduce its own challenges. Further research is suggested around the hierarchy found in IPS debriefing and how this translates to and from clinical practice.
Fostering translational research in chronic disease management: a logic model proposal
Authors: Lavertu Gardy , Diendere Ella , Légaré France , Zomahoun Hervé Tchala Vignon , Toi Alfred Kodjo , Leblond Mathilde , Rheault Nathalie , Audet-Walsh Étienne , Beaulieu Marie-Claude , Ben Charif Ali , Blanchette Virginie , Després Jean-Pierre , Gaudreau André , Rhéaume Caroline , Tremblay Marie-Claude , Paquette Jean-Sébastien 
Published: June 2022

AbstractTranslational research aims at reducing gaps between fundamental scientific discoveries and real-world applications. However, the trajectory of most scientific discoveries along the translation research continuum remains highly complex. Logic models are powerful tools that can help reduce this complexity. They are often used to lay out road maps and depict the relationship between activities and their intended effects. Few if any existing tools have been designed to guide the implementation and evaluation of collaborative models between community-based primary health care and biomedical research. To address this gap, we developed a logic model in two stages: 1) a literature review; and 2) the drafting and revision of the model by experts in the field. We describe its components, including objectives, inputs, activities, target groups, outputs, and results for a collaborative model involving fundamental biomedical research and primary health care practices. Our proposed logic model provides a road map that has the potential to reduce the complexity faced by translational research in chronic diseases by providing guidelines for decision-makers. Future work should attempt to validate the model before its broad-based implementation.
Prevalence and factors associated with thirst among postsurgical patients at University of Gondar comprehensive specialized hospital. Institution-based cross-sectional study
Authors: Belete Kumlachew Geta , Ashagrie Henos Enyew , Workie Misganaw Mengie , Ahmed Seid Adem 
Published: June 2022

Abstract
Introduction
Thirst is a powerfully distressing sensation that occurs most frequently in the immediate postoperative period. Postoperative thirst is prevalent, the moderate-to-severe type is estimated to affect 53.2–69.8% of patients and causes significant patient discomfort.

Objective
The objective of this study was to assess the prevalence, and factors associated with postoperative thirst among surgical patients in PACU at the University of Gondar Comprehensive Specialized Hospital from April 20 to June 27, 2021.

Methods
An institution-based cross-sectional study was conducted at the University of Gondar Comprehensive Specialized Hospital. A total of 424 participants were included in the study. Statistical analysis had performed using SPSS 26.00 version statistical software. Binary logistic regression analysis was performed to identify the association between the prevalence of postoperative thirst and independent variables and only variables with p-value < 0.2 were entered into the multivariable analysis. The strength of the association was presented by odds ratio and 95% Confidence interval. P-value < 0.05 was considered statistically significant.

Result
The prevalence of postoperative thirst among postsurgical patients was 59% (95% CI = 54.74–64.13). Inadequate preloading (Adjusted odes ratio (AOR) = 2.137 95% CI 1.260–3.624), prolonged Nil Per Os (NPO) time (AOR = 13.80 95% CI 2.93–65.37), general anesthesia (AOR = 3.90 95% CI 3.56–11.25), and axillary body temperature ≥ 37.5 °C (AOR = 8.07 95% CI 3.63–17.96) were significantly associated with postoperative thirst. Low room temperature (< 20 °C) was protective for the occurrence of postoperative thirst (AOR = 0.162 95% CI 0.37–0.707).

Conclusion and recommendations
The prevalence of postoperative thirst remains high and need commitment in close monitoring of PACU patients and immediate intervention. We also urge that high-level, ongoing research be conducted in this area, as postoperative thirst is a very common problem with a lot to discover.
Direct detection of iro B, stn and hil A virulence genes in Salmonella enterica serovar typhimurium from non-ripened cheese
Authors: Oladapo Omololu Daniel , Onifade Anthony Kayode , Bayode Michael Tosin 
Published: June 2022

Abstract
Background
Microorganisms' survival is based on the importance of growth factors found in the microbial environment, as well as their overwhelming appetite for survival which is controlled by their genetic material. This study was designed to investigate the virulence patterns of Salmonella enterica serovar typhimurium associated with soft cheese. Two hundred and sixty (260) soft cheese samples were collected from vendors at Akure, Ikare and Owo in Ondo State, Nigeria. Molecular characterization of six (6) Salmonella enterica isolates for the possession of iro B, stn, hil A and spv C gene were revealed through multiplex polymerase chain reaction (PCR) analysis with DNA extraction, polymerase chain reaction (PCR) amplification, sequencing and genome blasting, respectively.

Results
Four (4) possess only iro B; two (2) possess only stn gene; and another three (3) possess hil A gene as none of the isolates possess spv C gene. This study revealed antibiotic-resistant Salmonella enterica serovar typhimurium is present in locally made soft cheese samples. It also revealed that virulence genes comprising hil A, stn and iro B as well as plasmids-mediating antibiotic resistance are present in S. enterica serovar typhimurium found in locally produced cheese.

Conclusions
The presence of molecularly elucidated virulence genes comprising Iro B, Stn and Hil A makes the soft cheese potentially viable for pathogenicity. This study recommends food-borne salmonellosis should be constantly monitored with appropriate cleaning of preparatory paraphernalia in cheese production. Constant sensitization of nomads to improve their awareness on milk-borne zoonosis and its associated risk factors is needed.
Are there avoidable causes of early revision total knee arthroplasty?
Authors: Roof Mackenzie A. , Kreinces Jason B. , Schwarzkopf Ran , Rozell Joshua C. , Aggarwal Vinay K. 
Published: June 2022

Abstract
Introduction
Surgeons can improve their practice by understanding potentially avoidable reasons for early revision total knee arthroplasty (rTKA). The purpose of this study is to describe potentially avoidable indications for rTKA within 5 years of the index procedure.

Methods
This retrospective observational analysis utilized the rTKA database at a large, academic orthopedic specialty hospital to identify 167 consecutive rTKA from 2014 to 2019 performed within 5 years after primary TKA (pTKA). Two fellowship-trained arthroplasty surgeons reviewed the cases to classify them as potentially avoidable or unavoidable. Unavoidable indications for rTKA included infection/wound complication, arthrofibrosis, instability due to underlying collagen disease or significant weight loss, and any traumatic event leading to pTKA failure. Potentially avoidable indications included atraumatic instability, intraoperative fracture, metal allergy, and atraumatic patellar instability or maltracking. Aseptic loosening was considered avoidable if it occurred in the presence of component malpositioning or poor cementation technique.

Results
There were 112 (67.1%) unavoidable cases and 55 avoidable cases (32.9%). Of the unavoidable cases, there were 68 rTKA for infection or wound complications (60.7%), 23 for arthrofibrosis (20.5%), 6 for instability (5.4%), 6 for postoperative fracture (5.4%), 6 for aseptic loosening (5.4%), and 3 for extensor mechanism pathology following trauma (2.6%). Of the potentially avoidable rTKA, 24 were for aseptic loosening (43.7%), 23 for atraumatic instability (41.8%), 6 for atraumatic extensor mechanism pathology (10.9%), 1 for nickel allergy (1.8%), and 1 for intraoperative fracture (1.8%).

Conclusion
These findings indicate that over 30% of early rTKA are potentially avoidable. Interventions should focus on addressing potentially avoidable causes such as short-term aseptic loosening and instability to reduce the need for costly and resource-intensive rTKA.
Level of evidence III, retrospective observational analysis