bluuebunny/crossref_metadata_2025_split huggingface dataset:
Full-length chloroplast genome of Dongxiang wild rice reveals small single-copy region switching
Authors: Liang Jianguang , Chen Rui , Zhang Fantao , Wang Qian , Yang Yingxia , Lv Mingjie , Yan Shuangyong , Gao Shan 
Published: September 2022

BackgroundPlant chloroplast DNA (cpDNA) typically has a circular structure, including a large single-copy region (LSC), a small single-copy region (SSC) and two inverted repeats (IR1 and IR2). The organization of these four elementary regions LSC-IR1-SSC-IR2 is highly conserved across all plant cpDNAs. Very few structural variations (SVs) occurring at the elementary-region level have been reported.ResultsIn the present study, we assembled the full-length cpDNA of Dongxiang wild rice line 159 (DXWR159). Using the long PacBio subreads, we discovered a large inversion of SSC and a large duplication of IR in DXWR159 cpDNAs. Significantly, we reported for the first time forward and reverse SSCs of cpDNAs in similar proportions and named the frequent inversion of a whole SSC as SSC switching.ConclusionsOur study helps researchers to correctly assemble the chloroplast genomes. Our recombination model explained the formation of large SVs in cpDNAs and provided insights into a novel scientific question that if there are common mechanisms in the formation or translocation of all kinds of transposon-like elements (TLEs). We propose that: (1) large inversion is the most accepted mutation type of SVs in cpDNAs; (2) SSC switching ubiquitous occurs in plant cpDNAs; and (3) further investigation of molecular mechanism underlying SSC switching may reveal new driving forces for large SVs.
Influence of a special training process on the psychomotor skills of cadet pilots – Pilot study
Authors: Prokopczyk Adam , Wochyński Zbigniew 
Published: September 2022

ObjectivesThe aim of the pilot study was to check the influence of the training process on the Special Aviation Gymnastics Instruments (SAGI) on the improvement of the psychomotor skills, expressed as an increase in the percentage of ability to perform all tasks and the number of reels on a loop.Materials and methodsCadets - second year pilots (n = 20), male, mean age 20.8 years old, studying at the faculty of a pilot. Cadets were carrying out a 40-h special pilot training program on SAGI. They were subjected to two exercise tests (reels forward on looping), before and after the period of special training. Exercise tests were performed with the use of a diagnostic and training device used to assess psychomotor skills. During two tests, heart rate (HR) and blood pressure were measured. The obtained results were analysed statistically.ResultsThere was a statistically significant increase in the percentage of ability to perform all tasks (p &lt; 0.01) and a statistically insignificant increase in the number of reels forward on looping, in test II in relation to test I. A significant increase was found in the correct execution of arithmetic operations (p &lt; 0.05) in test II in relation to test I. In the remaining tests, an increase in results in test II was noted, but it was not statistically significant. There was a significant correlation between the percentage ability to perform all tasks and the number of completed reels in test I (p &lt; 0.05) and insignificant in test II. In test II, a statistically insignificant higher level of heart rate and blood pressure before and after the effort was noted, compared to test I.ConclusionIt was found that the training process on SAGI increased the psychomotority level by increasing the percentage of ability to perform all tasks and the number of reels, in test II in relation to test I.
The relation between coping style and posttraumatic growth among patients with breast cancer: A meta-analysis
Authors: Wan Xiao , Huang Haitao , Peng Qianwen , Zhang Yiming , Hao Jiwei , Lu Guangli , Chen Chaoran 
Published: September 2022

Posttraumatic growth (PTG) has been correlated with coping style among patients with breast cancer. However, to date, there is no consensus on the extent to which coping style is associated with PTG in patients with breast cancer. Therefore, we performed a meta-analysis to quantitatively synthesize previous findings. Based on the PRISMA method, this study employed a random effects model using the Stata software (version 16.0) to calculate the pooled correlation coefficient and examined a range of moderators: cancer stage, publication type, participants’ age, and coping style measurement tools. Relevant studies, published from inception to 9 March 2022, were identified through a systematic search in PubMed, Embase, Web of Science, PsycINFO, WANFANG DATA, Chinese National Knowledge Infrastructure (CNKI), and China Science and Technology Journal Database (VIP) databases. Finally, 20 studies involving 3,571 breast cancer patients were included in this investigation. The results showed a high positive relation between confrontation coping and PTG and a moderate positive relation between avoidance coping and PTG (confrontation: r = 0.456; avoidance: r = 0.291). Additionally, a moderate negative relation was identified between acceptance–resignation coping and PTG (r = –0.289). Publication type and coping style measurement tools moderated the relation between coping style and PTG among breast cancer patients. The findings indicated that breast cancer patients should either confront the disease or avoid coping with it according to their disease state, which would facilitate better growth. More studies, especially, large prospective studies, are warranted to verify our findings.Systematic review registration[https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022319107], identifier [CRD42022319107].
One uncertainty added on top of another: Challenges and resources of mothers of preterm infants during the COVID-19 pandemic
Authors: Shoshi Palmor Haspel , Tuval-Mashiach Rivka , Bin Nun Alona 
Published: September 2022

Aims and objectivesTo qualitatively explore COVID-19-related experiences of mothers of preterm infants in the Neonatal Intensive Care Unit (NICU), the main challenges they face, and the resources available for them.BackgroundThe birth of a preterm infant is a stressful event under otherwise normal circumstances. The outbreak of COVID-19, the uncertainty about the virus and how it spreads, and the restrictions imposed, may have exacerbated the stress of caring for a preterm infant.DesignRetrospective interviews.MethodsIn-depth interviews with 12 mothers of preterm infants who were hospitalized in the NICU at the time of study. The interview addressed challenges and resources related to coping with the pandemic. The interviews were transcribed and content analyzed, based on Lieblich et al’s model for narrative analysis. This research was conducted in accordance with the COREQ checklist.ResultsThe overarching experience shared by all mothers was accumulative stress caused by a combination of factors related to the infant’s health and COVID-19-related stressors. A central theme was the dissonance between the mothers’ expectations from the birth and infant, and the reality they encountered. Other themes included fear of infecting the infant, loneliness, and stress caused by the restrictions that disrupted daily routines. Resources included a sense of shared fate regarding the pandemic, improvements in the infant’s condition, religious faith, emotional support from the partner, and support from professional staff.ConclusionCaring for a preterm infant during a pandemic is a challenging experience on many levels. The loss of significant support resources puts mothers of these infants at a higher risk for psychological distress.Relevance to clinical practiceAwareness of mothers’ accumulative stress due to the COVID-19 pandemic may assist the staff in developing procedures that can alleviate parental stress, for example by enabling mothers to connect to each other, giving clear information to compensate for physical and social distancing and providing professional mental health support.
Why does subordinates’ negative workplace gossip lead to supervisor undermining? A moderated mediation model
Authors: Zeng Hao , Zhao Lijing , Li Jinsheng 
Published: September 2022

ObjectivesPrevious studies on negative workplace gossip have neglected the role of gossip targets of supervisors. The purpose of this paper is to deepen our understanding of how subordinates’ negative workplace gossip affects supervisors’ work-related behaviors. Drawing upon conservation of resource theory, the authors propose that subordinates’ negative gossip leads to supervisor emotional exhaustion. In turn, such emotional exhaustion provokes supervisors to exhibit undermining toward their subordinates. Additionally, the authors propose that a trait factor, namely, supervisor mindfulness, mitigates the relationship between such negative workplace gossip and supervisors’ emotional exhaustion.MethodData were collected from employees (e.g., subordinates) and their immediate supervisors in 35 organizations located in Jiangsu and Anhui Provinces in China. The data were obtained at three time points, each time interval was 2 weeks, and finally, 362 valid data points were obtained.ResultsThe following findings were obtained: (1) perceived subordinates’ negative gossip has a significant positive effect on supervisor undermining; (2) supervisor emotional exhaustion mediates the relationship between perceived subordinates’ negative gossip and supervisor undermining; and (3) supervisor mindfulness moderates the relationship between perceived subordinates’ negative gossip and supervisor emotional exhaustion and moderates the mediating effect of supervisor emotional exhaustion on the relationship between perceived subordinates’ negative gossip and supervisor undermining.ConclusionUsing multisource data and a moderated mediation model, we found that subordinates’ negative workplace gossip predicts supervisor undermining through supervisor emotional exhaustion. We also discovered that supervisor mindfulness can buffer the positive relationship between perceived subordinates’ negative workplace gossip and supervisor undermining. These findings have important implications for the literature on negative gossip in the workforce, especially the impact of subordinates’ negative workplace gossip on supervisors’ responses.
Coping strategies and their associated factors among caregivers of patients with schizophrenia in Kuantan, Malaysia
Authors: Kamarulbahri Tengku Mohd Saifuddin Tengku , Ariaratnam Suthahar , Nikmat Azlina Wati , Abdullah Nik Nairan , Khing Tan Lee 
Published: September 2022

BackgroundIt is essential for caregivers of schizophrenia patients to have effective coping mechanisms to deal with their own mental health. However, research on the factors that contribute to the coping mechanisms of caregivers is limited. The purpose of this study is to investigate the use of coping strategies and their correlations to socio-demographic features, psychological distress, and social support among the caregivers of patients with schizophrenia.MethodThrough the convenience sampling method, 331 caregivers of patients with schizophrenia participated in this cross-sectional study. The respondents comprised caregivers who had attended an outpatient psychiatric clinic, providing the socio-demographic data. The study involves three instruments: the Kessler's Psychological Distress (K10); the Multidimensional Scale of Perceived Social Support (MSPSS); and the Brief-COPE.FindingsUsing multivariable analysis, psychological distress was identified as a substantial independent predictor of emotion-focused, problem-focused, and avoidance coping strategies. Good social support was linked to the usage of problem-focused and emotion-focused coping strategies.ConclusionIt has been demonstrated that good social support and psychological distress are associated with coping strategies. More prospective and qualitative research is required to determine how coping strategies will be able to assist Malaysian caregivers to develop a more holistic approach to treating patients with schizophrenia.
Effects of Baduanjin imagery and exercise on cognitive function in the elderly: A functional near-infrared spectroscopy study
Authors: Yao Lianqiang , Sun Guoxiao , Wang Jun , Hai Yujuan 
Published: September 2022

ObjectiveCognitive function is essential in ensuring the quality of life of the elderly. This study aimed to investigate the effects of Baduanjin imagery and Baduanjin movement (a traditional Chinese health exercise, TCHE) on cognitive function in the elderly using functional near-infrared spectroscopy (fNIRS).Methods72 participants with a mean age of 66.92 years (SD = 4.77) were recruited for this study. The participants were randomly assigned to three groups: the Baduanjin imagery, the Baduanjin exercise, and the Control. Stroop task was used to record the accuracy and reaction times, and a near-infrared spectral brain imaging system was used to monitor the brain's oxy-hemoglobin concentration responses.Results(1) For the reaction times of Stroop incongruent tasks, the main effect of the test phase (F = 114.076, p &lt; 0.001) and the interaction effect between test phase and group (F = 10.533, p &lt; 0.001) were all significant. The simple effect analysis further demonstrated that the reaction times of the Baduanjin imagery group and Baduanjin exercise group in the post-test was faster than that in the pre-test (ps &lt; 0.001); (2) Analysis of fNIRS data showed the significant interaction effect (F = 2.554, p = 0.013) between the test phase and group in the left dorsolateral prefrontal cortex. Further analysis showed that, during the post-test incongruent tasks, the oxy-Hb variations were significantly higher in participants of the Baduanjin imagery group (p = 0.005) and Baduanjin exercise group (p = 0.002) than in the control group; For the right inferior frontal gyrus, the interaction between the test phase and group was significant (F = 2.060, p = 0.044). Further analysis showed that, during the post-test incongruent tasks, the oxy-Hb variations were significantly higher in participants of the Baduanjin imagery group than in the control group (p = 0.001).ConclusionBaduanjin imagery and exercise positively affect cognitive performance; Baduanjin imagery and exercise activated the left dorsolateral prefrontal cortex; Baduanjin imagery activated the right inferior frontal gyrus, while Baduanjin exercise could not.
Determinants of emotional distress in neonatal healthcare professionals: An exploratory analysis
Authors: Nazzari Sarah , Grumi Serena , Ciotti Sabina , Merusi Ilaria , Provenzi Livio , Gagliardi Luigi 
Published: September 2022

BackgroundHigh levels of mental health problems have been consistently reported among neonatal healthcare professionals. While studies suggest that personality, coping strategies and safety culture might contribute to the psychological wellbeing of healthcare professionals, they have not been systematically investigated in low-risk (i.e., neonatal wards; NWs) and high-risk (i.e., neonatal intensive care units; NICUs) neonatal contexts. The current study investigated potential predictors of professionals' emotional distress and whether they differ according to the work setting (i.e., NICUs vs. NWs).MethodsHealthcare professionals (N = 314) from 7 level-3 (i.e., NICUs) and 6 level-2 (i.e., NWs) neonatal units in Tuscany were included. Emotional distress (i.e., anxiety, depression, psychosomatic, post-traumatic stress symptoms and emotional exhaustion), Behavioral Inhibition System (BIS) and Behavioral Approach System (BAS) sensitivity, coping strategies and safety culture were assessed through well-validated, self-reported questionnaires.ResultsGreater BIS/BAS sensitivity, avoidance coping strategies and a sub-dimension of safety culture (i.e., stress recognition) were significantly associated with greater risk of emotional distress, whereas job satisfaction emerged as a protective factor. Three specific profiles of professionals in term of personality, coping and safety culture were identified and further predicted emotional distress. Neonatal wards and NICUs personnel presented different associations between personality, coping and safety culture.ConclusionThese findings highlighted significant modifiable contributors of neonatal mental healthcare professionals' wellbeing. Institutional initiatives that target these factors and, particularly, job satisfaction may promote professionals' emotional wellbeing and thus improve caring processes.
Clinical effect of day case arthroscopic surgery in tibial-eminence fracture in adults using button plates
Authors: Xu Xiaohui , Wang Huayi , Cui Fengguo , Guo Feng 
Published: September 2022

BackgroundThe tibial-eminence fracture (TEF) is an anterior cruciate-ligament avulsion fracture with a low incidence. Many surgical techniques have been described, but none of them allow early functional exercise, and there are many postoperative complications.PurposesThis study aimed to evaluate the early clinical efficacy and complications of day case arthroscopic-surgery treatment of adult TEF with button plates.MethodsWe retrospectively analyzed patients with TEF treated with arthroscopic surgery. Clinical subjective evaluation included International Knee Documentation Committee (IKDC) subjective score, Lysholm Knee Score, and Visual Analog Scale (VAS) score. Knee joint scores were evaluated by Lysholm score. Clinical objective assessment included the Lachman test, anterior-drawer test (ADT), IKDC, and range of motion. We assessed patient quality of life using a life summary table. Assessment of fracture healing and internal fixation was based on lateral x-rays of the knee joint. We measured and evaluated patient satisfaction at the last follow-up in accordance with Marsh criteria.ResultsAt final follow-up (average follow-up time, 28.23 ± 3.14 months), we evaluated results from 22 patients (22 knees). Average patient age during surgery was 33.64 ± 6.96 years. Average time from injury to surgery was 6.59 ± 1.47 h. Postoperative function was better than pre-operative function in all patients. IKDC subjective score, Lysholm score, and VAS score were better at final follow-up than before surgery. Differences in Lachman test and ADT scores before and after surgery were statistically significant. According to Intra-articular button position classification, 6 patients (6 knees) showed ideal position (A), 16 patients (16 knees) showed nearly ideal position (B), and none of the patients had nonideal position (C). The fractures of 22 patients healed completely; 2 patients had a 5°–10° knee joint dysfunction, and 1 had an abnormal knee sound. According to intra-articular button position classification, the rate of ideal position was 100%. Patient satisfaction rate was 81.8%.ConclusionDay surgery using double-button plates to treat TEF could achieve anatomical reduction, power and stability, as well as good clinical efficacy.
Prognostic value of glucose to lymphocyte ratio for patients with renal cell carcinoma undergoing laparoscopic nephrectomy: A multi-institutional, propensity score matching cohort study
Authors: Ni Jinliang , Li Ziye , Song Wei , Zhang Houliang , Wang Yidi , Zhang Yifan , Zhang Haipeng , Yang Guangcan , Xie Jun , Wang Keyi , Peng Bo , Mao Weipu 
Published: September 2022

BackgroundWe evaluated the prognostic value of preoperative blood glucose to lymphocyte ratio (GLR) in renal cell carcinoma (RCC) patients who underwent laparoscopic nephrectomy through a multi-institutional clinical study.MethodsA total of 420 patients with RCC from three medical centers from 2014 to 2019 were included in this study. The effect of GLR on overall survival (OS) and cancer-specific survival (CSS) in RCC patients was assessed by Kaplan-Meier survival curves, univariate and multivariate Cox regression analysis. Moreover, a 1:1 propensity score matching (PSM) analysis of different GLR groups was utilized to further confirm the prognostic ability of GLR.ResultsThe optimal cut-off value for GLR was 6.8. Patients were divided into high GLR and low GLR groups according to the optimal cut-off value. GLR was significant association with diabetes, cardiovascular disease and AJCC stage. High GLR predicted adverse OS (P = 0.002) and CSS (P &lt; 0.01) in RCC patients. Multivariate Cox regression analysis revealed that high GLR was an independent prognostic factor for OS [hazard ratio (HR): 2.389, 95% confidence interval (CI), 1.136–5.027, P = 0.008] and CSS (HR: 3.474, 95% CI, 1.555–7.761, P = 0.002). After PSM analysis of the patients in the high GLR and low GLR groups, high GLR still predicted poor OS (P = 0.021) and CSS (P = 0.037).ConclusionsHigh GLR is associated with adverse prognosis in RCC patients, and GLR can serve as an independent prognostic marker for OS and CSS in RCC patients receiving laparoscopic nephrectomy.
Case Report: Diagnosis of vertebral alveolar echinococcosis upon next-generation sequencing in a suspected tuberculosis
Authors: Song Tiange , Peng Shengkun , Zhou Xiaoli , Jiang Li , Zhang Jie 
Published: September 2022

IntroductionAlveolar echinococcosis (AE), caused by larval stages of Echinococcus multilocularis, is a rare zoonotic disease that mainly involves the liver. The diagnosis of extrahepatic AE is usually difficult. Here, we describe a rare case of vertebral alveolar echinococcosis with a suspected history of spinal tuberculosis, diagnosed by metagenomic next-generation sequencing (mNGS).Case PresentationA 44-year-old woman presented with repetitive neck and back pain, with a surgical history of suspected spinal tuberculosis. Magnetic resonance imaging (MRI) showed cystic masses in the craniocervical junction region and effusion around lumbar vertebrae. Multiple culture tests were performed to detect tuberculosis and other pathogens through puncture of the effusion and of cerebrospinal fluid, but the results were all negative. Finally, mNGS of the effusion fluid was performed and Echinococcus multilocularis were detected. The results were further confirmed by Sanger sequencing.ConclusionThis case emphasizes a role of mNGS in the diagnosis of infectious diseases with unknown pathogen. As a newly emerged sensitive and accurate diagnostic strategy, mNGS provides clinicians an opportunity to clarify pathogens in complicated infectious cases, especially in patients with a history of multiple infections.
Gram-Scale Preparation of Cannflavin A from Hemp (Cannabis sativa L.) and Its Inhibitory Effect on Tryptophan Catabolism Enzyme Kynurenine-3-Monooxygenase
Authors: Puopolo Tess , Chang Tanran , Liu Chang , Li Huifang , Liu Xu , Wu Xian , Ma Hang , Seeram Navindra P. 
Published: September 2022

Inhibitors targeting kynurenine-3-monooxygenase (KMO), an enzyme in the neurotoxic kynurenine pathway (KP), are potential therapeutics for KP metabolites-mediated neuroinflammatory and neurodegenerative disorders. Although phytochemicals from Cannabis (C. sativa L.) have been reported to show modulating effects on enzymes involved in the KP metabolism, the inhibitory effects of C. sativa compounds, including phytocannabinoids and non-phytocannabinoids (i.e., cannflavin A; CFA), on KMO remain unknown. Herein, CFA (purified from hemp aerial material at a gram-scale) and a series of phytocannabinoids were evaluated for their anti-KMO activity. CFA showed the most active inhibitory effect on KMO, which was comparable to the positive control Ro 61-8048 (IC50 = 29.4 vs. 5.1 μM, respectively). Furthermore, a molecular docking study depicted the molecular interactions between CFA and the KMO protein and a biophysical binding assay with surface plasmon resonance (SPR) technique revealed that CFA bound to the protein with a binding affinity of 4.1×10−5 M. A competitive SPR binding analysis suggested that CFA and Ro 61-8048 bind to the KMO protein in a competitive manner. Our findings show that C. sativa derived phytochemicals, including CFA, are potential KMO inhibitors, which provides insight into the development of therapeutics targeting the KP and its related pathological conditions.