bluuebunny/crossref_metadata_2025_split huggingface dataset:
Поражение костей у взрослых пациентов с болезнью Гоше I типа: распространенность, клинические особенности, костно-суставные осложнения и проблемы инвалидизации
Authors: Мамонов Василий Евгеньевич , Чабаева Ю. А. , Пономарев Р. В. , Лукина Е. А. 
Published: January 2025

BACKGROUND. Although highly effective enzyme replacement therapy (ERT) for Gaucher disease (GD) is accessible and affordable, orthopedic issues in patients appear often to be the main factor leading to their disability. Bone lesions are essentially observed in 100 % of GD patients. The nature and severity of bone and joint changes, however, are reported to be extremely heterogeneous, whereas their incidence and clinical features have until now remained underresearched.
AIM. To assess the incidence and clinical value of bone and joint lesions which complicate the course of GD type 1 with bone involvement.
MATERIALS & METHODS. This trial enrolled 267 GD type 1 patients aged 17–85 years (median 38 years), who were followed-up at the National Research Center for Hematology from 2004 to 2024. There were 109 men and 158 women. The analysis focused on the following MRI findings: bone marrow infiltration (BMI), Barnett-Nordin cortical index (BNI), bone deformity, osteolytic and osteonecrotic lesions, as well as orthopedic pathologies progressing to disability.
RESULTS. BMI was identified in 213/267 (79.8 %) patients. Erlenmeyer flask-like femoral expansion was observed in 95 % patients (n = 254). The mean BNI was 42.9 % (the normal BNI in adult population exceeds 45 %). Low BNI values were associated with osteolytic lesions, disability, and BMI. No significant association of decreased BNI was seen with osteonecroses. The incidence of osteolytic lesions was 11.2 % (n = 30). New osteolytic lesions were reported neither in ERT nor in substrate reduction therapy recipients. Medullary osteonecrosis was detected in 206/267 (77.15 %) patients, and epiphyseal osteonecrosis was identified in 74/267 (27.72 %) patients. Along with osteonecroses, in contrast to osteolytic manifestations, new lesions (n = 8) were reported to occur under long-term (> 5 years) ERT. The rate of bone fractures was 6.7 % (n = 18). In 18 patients, 64 fractures were observed at various locations. Disability associated with bone and joint complications of GD occurred in 58/267 (21.72 %) patients. After reconstructive surgery, including total articular replacement arthroplasties, 37 patients achieved complete functional recovery.
CONCLUSION. Bone changes are essentially observed in all adult patients with GD type 1. However, clinical severity and disability of patients depend on secondary bone and joint complications, among them arthroses resulting from epiphyseal osteonecrosis, pathologic fractures, bone and joint infections. These complications developing on effective ERT were identified in 67/267 patients, which accounted for 25 %.
Proteomics Analysis of Five Potential Plasma-derived Exosomal Biomarkers for Acute Myocardial Infarction
Authors: Xu Shasha , Zhai Yi , Wang Chen , Zhang Yang , Liu Xiaowei , Jiang Jianjun , Mi Yafei 
Published: January 2025

Aims:
This study was to explore the relationship between plasma exosomes
and Acute myocardial infarction (AMI).

Background:
Acute myocardial infarction (AMI) is one of the most common cardiovascular
complications. Recent studies have shown that exosomes play a crucial role in the
development and progression of cardiovascular diseases. However, there is a lack of relevant
research on the relationship between plasma exosomes and AMI.

Objective:
This study was designed to explore the relationship between plasma exosomes
and AMI.

Methods:
This retrospective study collected the basic clinical data of patients with AMI
(n = 10), stable angina pectoris (SAP, n = 10), and noncoronary heart disease (CON, n =
10) at the Department of Cardiovascular Medicine at Taizhou Hospital (Zhejiang, China,
2021.01 to 2021.12). Proteomics was used to systematically screen the differential
proteins of plasma exosomes in patients with clinical AMI, SAP, and CON. Then, the results
were further verified using parallel reaction monitoring (PRM).

Results:
Five of all the differentially expressed proteins (DEPs) were quantified by
PRM. Compared with the CON group, heparin cofactor 2 (SERPIND1), mannan-binding
lectin serine protease 1 (MASP1), ficolin-2 (FCN2), and α1-Microglobulin/bikunin
precursor (AMBP) were upregulated in patients with AMI and SAP, with a higher expression
in AMI than in SAP. Additionally, human leukocyte antigen (HLA-C) was
downregulated in both exosomes and plasma.

Conclusion:
The expression of four plasma exosome biomarkers in AMI and SAP patients
was higher than that in noncoronary heart disease (NCHD) patients. HLA-C was
downregulated in both exosomes and plasma, showing a potential to serve as a new candidate
target for the detection and therapy of AMI.
Predicting microRNAs and their Target Genes Involved in Sepsis Pathogenesis
by using Bioinformatics Methods
Authors: Musavi Maryam , Haftcheshmeh Saeed Mohammadian , Fazel Hadi , Momtazi-Borojeni Amir Abbas 
Published: January 2025

Introduction:
Sepsis, like neutropenic sepsis, is a medical condition in which our body overreacts
to infectious agents. It is associated with damage to normal tissues and organs by the immune system, which
leads to the spread of inflammation throughout our body. Of note, microRNAs (miRNAs) have been found to
have a critical role in the sepsis progression. Such miRNAs are registered in the miRNA databases, such as
Gene Expression Omnibus (GEO), with a specific identifier and unique characteristics. There is also computational
software, such as TargetScan, that are broadly employed for the analysis of miRNAs, including their
identification, target prediction, and functional analysis.

Methods:
The current In-silico study aimed to predict miRNAs involved in sepsis progression. To this end, the
GEO database was employed to find the sepsis-related genome profile. Afterward, down-regulated genes were
selected for further bioinformatics analysis with the assumption that their decreased expression is associated
with an increased sepsis progression. The miRNAs complementary to the selected genes were then predicted
using TargetScan software. Based on the current In-silico analysis, seven miRNAs, including hsa-miR-325-3p,
hsa-miR-146a-3p, hsa-miR-126-5p, hsa-miR-22-3p, hsa-miR-223-3p, hsa-miR-145-5p, and has-miR-181 family,
were predicted to participate in sepsis pathogenesis. Among the predicted miRNAs, hsa-miR-325-3p has
not been previously predicted or validated to be involved in septic conditions.

Results:
Our prediction results showed that hsa-miR-325-3p may target genes implicating in both anti-(ETFB
gene) and pro-inflammatory (TCEA1 and PTPN1 genes) responses, suggesting it is an immune hemostasis regulator
during sepsis inflammation. Although the role of other predicted miRNAs has been already validated in
the sepsis pathogenesis, the current study predicted new targets of these miRNAs, which have not been reported
by previous in-silico or experimental studies on sepsis and other pathogenic conditions. Notably, other miRNAs,
including hsa-miR-146a-3p, hsa-miR-126-5p, hsa-miR-22-3p, hsa-miR-223-3p, and hsa-miR-145-5p
were predicted to target genes participating in inflammatory responses, including BLOC1S1, POLR2G, PTPN1,
TCEA1, and CCT3.

Conclusion:
In conclusion, the results of the present study can provide promising targets as therapeutic and diagnostic
tools to treat and manage inflammation sepsis, such as neutropenic sepsis. However, these findings
should be further evaluated in experimental studies to find their exact effects and underlying mechanisms.
Application of Chitosan-based Nanogel in Cancer Nanomedicine
Authors: wang yue , Lou Xiaoli , Yang Liyuan , Hou Yanqiang 
Published: January 2025

Abstract:
Chitosan is a kind of natural material with many unique physicochemical and biological properties
related to antibacterial, antioxidant, and chelating. In recent years, chitosan-based nano gels (CS-NG) have
been widely used in the field of cancer nanomedicine due to their excellent characteristics including biodegradability,
biocompatibility, flexibility, large surface area, controllability, high loading capacity, and especially it
can be engineered to become stimuli-responsive to tumor environments. In this review, we summarized the
main synthesis approaches of CS-NGs including radical polymerization, self-assembly, microemulsion, and
ionic gelation methods. These novel CS-NGs are applied in cancer nanomedicine serving as drug delivery,
gene delivery, and bioimaging. Besides, we proposed our perspectives regarding the clinical development of
CS-NGs cancer nanomedicine applications.
A Review: Synthetic Pathways and Biological Activities of Oxo-imidazoline Derivatives in New Millennium
Authors: Sharma Vipasha , Nain Sumitra 
Published: January 2025

Abstract:
Imidazoline is often a heterogeneous 5-membered ring structure with two nitrogen atoms. The heterocyclic
compounds with an imidazoline moiety are a promising scaffold for investigating medicinal potential.
The imidazoline nucleus with an oxo group at the 5th, 2nd, or 4th position exhibits a wide spectrum of antibacterial
and antifungal activity, as well as antiviral, anti-inflammatory, anticancer, analgesic, antihypertensive,
antioxidant, and enzyme-inhibiting properties. Oxo-imidazoline derivatives are a fascinating class of chemicals
that have attracted a lot of research recently because of their numerous biological actions. The primary objective
of this paper is to review the synthesis and diverse biological activity of oxo-imidazoline derivatives literature
from previous years. The synthesis of oxo-imidazoline derivatives is a prominent topic of study in organic
chemistry because of their wide range of biological activity and possible medical uses. This review provides an
extensive overview of synthetic pathways employed to prepare oxo-imidazoline derivatives with excellent biological
activities. Oxo-imidazoline derivatives are synthesized using a variety of techniques, including oxidative
cyclization, amino acid cyclization, base-catalyzed, diamine ring closure metal-catalyzed cyclizations, and so
on are discussed in this review. This review also emphasizes the importance of synthetic methodology development
in enabling efficient access to oxo-imidazoline derivatives and their analogs, allowing for further investigation
of their biological and pharmacological properties. This review also aims to provide a comprehensive
overview of the various biological properties such as anti-microbial (anti-fungal & anti-bacterial), anti-cancer,
antioxidant, anti-inflammatory, analgesics, anti-virus, and miscellaneous activities (listed in Table 1) exhibited
by potent oxo imidazoline derivatives. More than 50 scientific articles presenting more than 80 compounds
have been reviewed. Figures and tables provide the most pertinent data that have been carefully extracted and
organized.
Macrophage HERC6 Promotes NAFLD: Integrated Analyses of Mendelian Randomization and Single-Cell Transcriptome
Authors: Zou Shengyi , Du Xuan , Lu Wen , Shi Bimin 
Published: January 2025

Aims and Objectives:
This study aimed to explore the relationship between HERC6-
associated immune response and Non-Alcoholic Fatty Liver Disease (NAFLD) and to screen
drug candidates for novel treatments.

Materials and Methods:
Mendelian Randomization (MR) was performed to test the relationship
between a genetically predicted increase in HERC6 expression and the development of NAFLD.
A single-cell RNA-seq profile of liver tissue with histological characteristics (GSE168933) was
obtained. The immune cell type with the highest HERC6 score was identified, and the HERC6
scores across hepatic steatosis, ballooning, and lobular inflammation were compared. Bulk RNA
data of liver samples from obese patients who underwent diet restriction or bariatric surgery
(GSE83452) was deconvoluted, and the proportions of distinct HERC6-high immune cell subpopulations
were estimated. The baseline and follow-up differences were compared to evaluate
the effectiveness of different therapies. Finally, a single-cell drug susceptibility assessment was
performed to screen drug candidates targeting HERC6-associated immune cells.

Results:
MR results showed that an increase in HERC6 expression was associated with an increase
in low-density lipoprotein cholesterol (LDL-C) level and the development of NAFLD.
The HERC6 score on monocyte-derived macrophage was the highest and positively correlated
with hepatic steatosis, ballooning, and lobular inflammation. The HERC6-high macrophage fraction
significantly decreased after bariatric surgery but not diet therapy. Triptolide was identified
as a potential drug for NAFLD targeting HERC6-high macrophages.

Conclusion:
The results highlighted the role of hepatic macrophage HERC6 in NAFLD
CAR-T Cell Therapy: Pioneering Immunotherapy Paradigms in Cancer Treatment
Authors: Borah Dhitri , Hojaisa Sibasree , Sarma Indira , Mavi Anil Kumar , Singh Tejveer , Gulati Anita , Goswami Ravi Kumar , Maru Saurabh 
Published: January 2025

Abstract:
The world's one of the major causes of death are cancer. Cancer is still a complex
disease over the years that needs to be cured. Traditional cytotoxic approaches, although they
have been implemented for years for treating neoplastic diseases, yet are limited due to the intricacy
and low efficiency of cancer cells. Researchers are thus compelled to seek more potent
therapeutic strategies. Chimeric antigen receptor (CAR-T) cell therapy is one such innovative
insight where T lymphocytes are altered genetically to target cancer cells. Despite the outstanding
accomplishment in patients with haematological malignancies, CAR-T cell treatment has
demonstrated minimal impact on solid tumours due to a number of obstacles, including proliferation,
stability, trafficking, and fate within tumors. Furthermore, interactions between the host
and tumour microenvironment with CAR-T cells significantly alter CAR-T cell activities. Designing
and implementing these treatments additionally also requires a complex workforce.
Overcoming these significant challenges, there is a requirement for innovative strategies for
developing CAR-T cells with greater anti-tumour efficacy and reduced toxicity. In this chapter,
the current advancement in CAR-T cell technology in order to increase clinical efficacy in both
solid tumors and haematological, as well as possibilities to conquer the limits of CAR-T cell
therapy in both solid and haematological tumours has been discussed.
Understanding Tankyrase Inhibitors and Their Role in the Management of Different Cancer
Authors: Das Sinjini , Byran Gowramma , Biswas Kaushik , Rajagopal Kalirajan 
Published: January 2025

Abstract:
Cancer manifests as uncontrolled cell proliferation. Tankyrase, a poly(ADP-ribose) polymerase member, is vital in Wnt signal transmission, making it a promising cancer therapy target. The Wnt/β-catenin pathway regulates critical biological processes like genomic stability, gene expression, energy utilization, and apoptosis. Its dysregulation contributes to cancer development. Targeting tankyrase within this pathway holds the potential for inhibiting aberrant cell growth and promoting programmed cell death, offering a promising avenue for cancer treatment. ADP-ribosylation, a reversible process, modifies proteins post-synthesis, regulating diverse cellular signaling pathways. Transferase enzymes like mono and poly(ADP- ribosyl) transferases transfer ADP-ribose from NAD+ to specific amino acid side chains or ADP-ribose units on target proteins. Blocking tankyrase has emerged as a promising strategy in cancer treatment. This article reviews recent advancements in developing novel tankyrase inhibitors. It delves into structure-activity relationships, molecular docking, polypharmacology profiles, and binding mechanisms at the active site. Insights into lead structure development aid in designing potent anti-cancer medications, shedding light on promising avenues in cancer therapy.
S100A8 and S100A9–Critical Mediators of Inflammation in Arthritis
Authors: Paramasivam Sivasakthi , Perumal Senthamil Selvan , Ekambaram Sanmuga Priya 
Published: January 2025

Abstract:
Damage-associated molecular Patterns (DAMP) released on the onset of tissue injury
interact predominantly with pattern-recognizing receptors (PRRs) and multiple receptors to trigger
and mediate inflammation and play a critical role in the progression of various diseases. Among
the multiple DAMPs, S100A8 (MRP-8) and S100A9 (MRP-14) are low molecular weight calcium-
binding proteins primarily expressed in innate immune cells such as myeloid cells: neutrophils,
monocytes, keratinocytes, and early differentiation states of macrophages giving them
the name Myeloid related proteins (MRP). S100A8 and S100A9 can exist both as homo- and heterocomplexes
– shown to elicit different functions in cellular physiology, chemotaxis, phagocyte
migration and modulation of various macrophage functions, apoptosis in cancer, and activation of
NF-κB pathway. S100A8 and S100A9 have been shown to trigger innate immunity via TLR-4 signaling,
thereby increasing the production of proinflammatory cytokines and other downstream effectors
of the cascade. An influx in S100A8 and S100A9 proteins has been implicated in the most
prevalent arthritic conditions, such as rheumatoid arthritis (RA) and osteoarthritis (OA). Hence,
the primary objective of the review is to provide a comprehensive and systematic analysis imparting
insights into the role of S100A8 and S100A9 proteins as mediators of inflammation in RA and
OA.
A Review of the Protective Effects of Rosemary and its Main Ingredients Against Cancer by Modulating Nrf2 Signaling
Authors: Amiri-Ardekani Ehsan , Radfar Amirreza , Tavakoli-Far Fatemeh , Farkhondeh Tahereh , Aschner Michael , Samini Fariborz , Samarghandian Saeed 
Published: January 2025

Abstract:
Rosemary, as a medicinal plant, contains valuable compounds such as rosmarinic acid,
rosanol, caronic acid, and carnosol. In this study, we summarized and summarized studies related
to the anticancer effects of rosemary and its main ingredients by activating Nrf2. For this purpose,
we searched the main databases including Scopus, PubMed, Web of Science, and MEDLINE until
2024. It was found that Rosemary and its main ingredients affect Nrf2 signaling activity, a pathway
that is induced in response to oxidative stress conditions to reduce reactive oxygen species
(ROS). Nrf2-related effects of Rosemary and its main ingredients have been studied in different
models of diseases, including cancers. These compounds also induce apoptosis and increase antioxidant
enzyme activity downstream in favor of cancer cell line death. The induction of Nrf2 activity
via activating protein kinase RNA-like endoplasmic reticulum kinase results in increased
phosphorylation at site threonine, downregulation of iNOS, enhancement of nerve growth factor,
and activating transcription factor 4 protects cells against carcinogenesis.
The Impact of Health Literacy on Knowledge, Attitude, and Practice Toward COVID-19 among Elderly Women: A Cross-Sectional Study in Iran
Authors: Ranjbar Afsaneh , Rahmani Ali Hasan , Mohamadi Solmaz , Abedi Parvin , Rahmanian Vahid , Javadifar Nahid , Jamali Safieh 
Published: January 2025

Background and Objectives:
The coronavirus has become a significant danger to human
health. Health literacy is a major factor influencing health outcomes. The aim of this study was to
assess health literacy and knowledge, attitude, and practice (KAPs) toward COVID-19 among elderly
women.

Methods:
This descriptive-analytical study was conducted on elderly women living in Jahrom City
in 2022. Participants responded to questions regarding sociodemographics, the Health Literacy
Scale, and a researcher-developed knowledge, attitude, and practice (KAP) questionnaire. Descriptive
statistics, one-way analysis of variance, independent sample T-test, Tukey post hoc test, and
multiple linear regression were employed to analyze the data.

Results:
The study participants had a mean age of 69.73 ± 7.17 years, with 38.53% aged over 70
years. Notably, 83.3% of participants expressed trust in information from friends and relatives. The
average health literacy score among the women was 75.79 ± 20.36, and 40.7% had high health literacy,
25.3% had adequate health literacy, 18% had somewhat insufficient health literacy, and 16%
had inadequate health literacy. The majority, 96%, had good awareness, 94.7% had a positive attitude,
and 87.4% had good performance regarding the prevention of COVID-19. The results revealed
that an increase of one unit in knowledge and practice scores corresponded to a respective
increase of 0.485 (p<0.001) and 0.274 (p=0.001) in the average health literacy score. Furthermore,
age (<0.001), education (<0.001), and occupation (<0.001) of participants were significantly associated
with knowledge and practice related to COVID-19.

Conclusion:
The study findings underscored the positive correlation between high health literacy
and individuals' awareness, practices, age of women, education, occupation, source of information,
and marital status. Enhancing people's awareness emerges as a key strategy to bolster health literacy
levels in the population.
In vitro and In silico Degradation Study Applied to the Carbapenems Meropenem and Imipenem
Authors: Carlos Graciela , Barbosa Fábio de Souza , Piton Yasmin Vendruscolo , Garcia Solange Cristina , Arbo Marcelo Dutra , Paula Fávero Reisdorfer , Garcia Cássia Virginia , Steppe Martin , Mendez Andreas Sebastian Loureiro 
Published: January 2025

Background:
Imipenem and meropenem are the carbapenem antibiotics most commonly
used for the treatment of severe bacterial infections. Their stability behavior, which focuses on degradation
products, has been continually reported. Strategies based on in silico prediction have been
explored as an additional tool that provides additional information about drug stability.

Methods:
A comparison between experimental and predictive data, with a focus on stability and
degradation products, was performed by examining the literature data and the in silico results. The
experimental data were obtained from the literature throughdatabase searches. The in silico degradation
prediction was performed through the software Zeneth 9.0, which strictly focused on degradation
products. With a focus on stability, degraded samples of meropenem and imipenem were investigated
in terms of biological safety, applying an in vitro cytotoxicity assay by MTT reduction and
neutral red uptake.

Results:
From the literature survey, it was found that 11 major degradation products for both carbapenems
mainly formed from modifications on the b-lactam ring and dimerization. By using the
Zeneth software, a large number of substances were predicted, considering the probability of occurring
and the selected stressors. Even in low numbers, the comparative results indicated similarity in 4
degradation products, with high probability. About cytotoxicity, the cell viability by MTT assay was
significantly reduced for imipenem samples degraded during 4 hours. For meropenem, applying
neutral red uptake assay, a reduction of cell viability was observed in samples stored for 4 hours.

Conclusion:
Drug stability assay includes different approaches, which can be used favorably from
the interaction between all areas of interest, including experimental practice and predictive modelling
assay.