bluuebunny/crossref_metadata_2025_split huggingface dataset:
Level and relationships of academic skills and family functions with academic performance during Covid 19 pandemic
Authors: Alammar Muath A. , Ram Dushad , Almansour Ibrahim Abdulrahman , Aljammaz Abdulrhman Saad 
Published: December 2022

AbstractBackgroundThe research indicates that academic skills and family function may influence academic achievement. The Covid 19 epidemic has impacted regular academic and family function. However, there is a dearth of studies evaluating the relevance of academic skills and family function on college students' academic achievement. This research was done to determine the levels and relationships between academic achievement and study skills and family functions. Two hundred seventy-nine college students were assessed with Sociodemographic and academic proforma, Study Skills Assessment Questionnaire (SSAQ), and The McMaster Family Functioning Scale (MFFS).ResultsResults revealed that the mean score of SSAQ and MFFS were 179.92 and 17.88, respectively. Multiple regression analysis showed that the score of reading skills and the MFFS score statistically significantly predicted the score of the exam.ConclusionsOn the basis of this study's results, it is possible to conclude that reading skills and family functioning may influence academic success.
A dual tracer [11C]PBR28 and [18F]FDG microPET evaluation of neuroinflammation and brain energy metabolism in murine endotoxemia
Authors: Palandira Santhoshi P. , Carrion Joseph , Turecki Lauren , Falvey Aidan , Zeng Qiong , Liu Hui , Tsaava Tea , Herschberg Dov , Brines Michael , Chavan Sangeeta S. , Chang Eric H. , Vo An , Ma Yilong , Metz Christine N. , Al-Abed Yousef , Tracey Kevin J. , Pavlov Valentin A. 
Published: December 2022

AbstractBackgroundBrain metabolic alterations and neuroinflammation have been reported in several peripheral inflammatory conditions and present significant potential for targeting with new diagnostic approaches and treatments. However, non-invasive evaluation of these alterations remains a challenge.MethodsHere, we studied the utility of a micro positron emission tomography (microPET) dual tracer ([11C]PBR28 – for microglial activation and [18F]FDG for energy metabolism) approach to assess brain dysfunction, including neuroinflammation in murine endotoxemia. MicroPET imaging data were subjected to advanced conjunction and individual analyses, followed by post-hoc analysis.ResultsThere were significant increases in [11C]PBR28 and [18F]FDG uptake in the hippocampus of C57BL/6 J mice 6 h following LPS (2 mg/kg) intraperitoneal (i.p.) administration compared with saline administration. These results confirmed previous postmortem observations. In addition, patterns of significant simultaneous activation were demonstrated in the hippocampus, the thalamus, and the hypothalamus in parallel with other tracer-specific and region-specific alterations. These changes were observed in the presence of robust systemic inflammatory responses manifested by significantly increased serum cytokine levels.ConclusionsTogether, these findings demonstrate the applicability of [11C]PBR28 - [18F]FDG dual tracer microPET imaging for assessing neuroinflammation and brain metabolic alterations in conditions “classically” characterized by peripheral inflammatory and metabolic pathogenesis.
Spatiotemporal dynamics of the microbial diversity on salt-preserved goatskins assessed by culturing and 16S rRNA gene amplicon sequencing
Authors: Li Xiaoguang , Sen Keya , Zhang Yuqin , Tian Yongqiang , Shi Bi 
Published: December 2022

AbstractWet-salted skin, as a special artificial high-salt environment, is rich in protein, fat, collagen and other nutrient substrates, and is a rich resource of halotolerant and halophilic microorganisms. However, knowledge gaps regarding the microbial community structure and inter taxa associations of wet-salted skin are large. In this study, the spatiotemporal dynamics and community structure of microorganisms present on wet-salted goatskins were investigated using 16S rRNA gene amplicon sequencing and culturable technique. Alpha diversity analysis based on Sobs, Chao, Ace and Shannon indices revealed that microbial diversity on the wet-salted goatskins exhibited a trend of ‘down → up → down → flat’ with time. During preservation, genera belonging to the bacteria domain such as Acinetobacter, Weissella and Streptococcus were slowly dying out, whereas those belonging to halophilic archaea such as Natrialba and Haloterrigena were gradually flourishing. Moreover, to resist high-salt stress, microorganisms on the wet-salted goatskin gradually migrated from the outside to the inside, eventually leading to the microbial diversity inside the skin being the same as or even higher than that on the skin surface. Venn diagram analysis revealed that the strains of some genera, including Psychrobacter, Salimicrobium, Salinicola, Ornithinibacillus, Halomonas, Bacillus and Chromohalobacter, were distributed throughout the interior and exterior of the wet-salted goatskin and existed during various periods. Accordingly, 45 protease-producing halophilic or halotolerant microorganisms were isolated and screened from the wet-salted goatskin using the gradient dilution plate method. Importantly, 16S rRNA genes of some bacteria exhibited less than 99.5% similarity to valid published species, indicating that they likely are novel species and have a good potential for application.
Graphical Abstract
Model parameter design for modeling surface topography in VTI elastic finite-difference near-surface simulations
Authors: Zhou Xuhui , Huo Shoudong , Wang Hao , Dong Shuli , Liang Yao , Cao Jian 
Published: December 2022

The near-surface anisotropy is one of the factors leading to an inaccurate velocity estimation in the shallow area, which has a strong impact on the static correction of land data and seismic imaging of the subsurface in depth. To investigate the anisotropy influence, the consideration of medium anisotropy in near-surface seismic modeling becomes necessary. In the oil and gas industry, finite-difference (FD) methods are commonly used for seismic wave modeling, thanks to their simplicity, accuracy, and high efficiency. However, for near-surface modeling, the implementation of free-surface boundary conditions and nonflat topography representation are the two main obstacles preventing the effectiveness of FD methods. To address these two issues in the vertical transversely isotropic anisotropic scenarios, we have developed a simple and efficient method for the discrete model design which can be easily applied to the conventional Cartesian-grid FD modeling. Our method involves: (1) the parameter-averaging method for implicitly implementing the stress-free condition by a modification of model anisotropy parameters near the (non) flat free-surface boundary and (2) an independent wavefield superposition with modeling results of different parameter configurations to accurately represent the rugged topography and significantly reduce the staircase diffractions caused by a staircase approximation of continuous surface in the Cartesian-grid discretization. For validation of this method, we conduct several numerical tests in 2D and 3D spaces. The accuracy is demonstrated by a comparison of the spectral-element solutions of SPECFEM in modeling the seismic wave propagation in the presence of an irregular free surface. From the aspect of computational efficiency, it is more promising in practical applications due to the use of wavefield superposition strategy in this method which does not require finer spatial sampling to eliminate the staircase diffractions.
Stability and Function of Extracellular Vesicles Derived from Immortalized Human Corneal Stromal Stem Cells: A Proof of Concept Study
Authors: Lyu Ning , Knight Robert , Robertson Sarah Y. T. , Dos Santos Aurelie , Zhang Chi , Ma Chao , Xu Jianjiang , Zheng Jie , Deng Sophie X. 
Published: December 2022

AbstractWith significant advancement and development of extracellular vesicle (EV)-based therapies, there is a growing need to understand how their storage affects their physical and functional characteristics. EVs were isolated from the conditioned medium of a corneal stromal stem cell line (imCSSC) using Total Exosome isolation kit (TEI) and ultracentrifugation (UC) combined protocol. Purified EVs were stored at 4°C, − 80°C, room temperature (RT) after lyophilization with or without trehalose for 4 weeks. EVs stored at − 80°C and RT (lyophilization with trehalose) demonstrated a comparable morphology, while the freeze-dried samples without trehalose showed aggregation and degradation under a transmission electron microscope (TEM). Lyophilized samples without trehalose demonstrated a decreased particle concentration, recovery rate and protein concentration, which was remediated by the addition of trehalose. EVs stored at − 80℃ showed no change in the protein expression of CD9, CD63, and CD81. Regardless of the storage condition, all EV samples investigated reduced inflammation, as well as inhibited expression of fibrotic markers in vitro. Lyophilization of EVs with trehalose was a feasible storage method that retained the physical property and in vitro biological activities of EVs after 4 weeks of storage, while − 80°C offered the best retention of imCSSC-derived EV physical properties. For the first time, this data demonstrated a practical and translatable method for the storage of CSSC-derived EVs for clinical use.
Graphical Abstract
Cooling the Tropics
Authors: Hobart Hi'ilei Julia Kawehipuaakahaopulani 
Published: December 2022

Beginning in the mid-1800s, Americans hauled frozen pond water, then glacial ice, and then ice machines to Hawaiʻi—all in an effort to reshape the islands in the service of Western pleasure and profit. Marketed as “essential” for white occupants of the nineteenth-century Pacific, ice quickly permeated the foodscape through advancements in freezing and refrigeration technologies. In Cooling the Tropics Hiʻilei Julia Kawehipuaakahaopulani Hobart charts the social history of ice in Hawaiʻi to show how the interlinked concepts of freshness and refreshment mark colonial relationships to the tropics. From chilled drinks and sweets to machinery, she shows how ice and refrigeration underpinned settler colonial ideas about race, environment, and the senses. By outlining how ice shaped Hawaiʻi’s food system in accordance with racial and environmental imaginaries, Hobart demonstrates that thermal technologies can—and must—be attended to in struggles for food sovereignty and political self-determination in Hawaiʻi and beyond.
Duke University Press Scholars of Color First Book Award Recipient
Chemokine signaling synchronizes angioblast proliferation and differentiation during pharyngeal arch artery vasculogenesis
Authors: Liu Jie , Zhang Mingming , Dong Haojian , Liu Jingwen , Mao Aihua , Ning Guozhu , Cao Yu , Zhang Yiyue , Wang Qiang 
Published: December 2022

ABSTRACT
Developmentally, the great vessels of the heart originate from the pharyngeal arch arteries (PAAs). During PAA vasculogenesis, PAA precursors undergo sequential cell fate decisions that are accompanied by proliferative expansion. However, how these two processes are synchronized remains poorly understood. Here, we find that the zebrafish chemokine receptor Cxcr4a is expressed in PAA precursors, and genetic ablation of either cxcr4a or the ligand gene cxcl12b causes PAA stenosis. Cxcr4a is required for the activation of the downstream PI3K/AKT cascade, which promotes not only PAA angioblast proliferation, but also differentiation. AKT has a well-known role in accelerating cell-cycle progression through the activation of cyclin-dependent kinases. Despite this, we demonstrate that AKT phosphorylates Etv2 and Scl, the key regulators of angioblast commitment, on conserved serine residues, thereby protecting them from ubiquitin-mediated proteasomal degradation. Altogether, our study reveals a central role for chemokine signaling in PAA vasculogenesis through orchestrating angioblast proliferation and differentiation.
Effects of Face Masks on the Multiple Dimensions and Neurophysiological Mechanisms of Exertional Dyspnea
Authors: FERGUSON OLIVIA N. , MITCHELL REID A. , SCHAEFFER MICHELE R. , RAMSOOK ANDREW H. , DHILLON SATVIR S. , DOMINELLI PAOLO B. , MOLGAT-SEON YANNICK , GUENETTE JORDAN A. 
Published: December 2022

ABSTRACT

Introduction
During the coronavirus disease 2019 pandemic, public health officials widely adopted the use of face masks (FM) to minimize infections. Despite consistent evidence that FMs increase dyspnea, no studies have examined the multidimensional components of dyspnea or their underlying physiological mechanisms.

Methods
In a randomized crossover design, 16 healthy individuals (n = 9 women, 25 ± 3 yr) completed incremental cycling tests over three visits, where visits 2 and 3 were randomized to either surgical FM or no mask control. Dyspnea intensity and unpleasantness were assessed throughout exercise (0–10 Borg scale), and the Multidimensional Dyspnea Profile was administered immediately after exercise. Crural diaphragmatic EMG and esophageal pressure were measured using a catheter to estimate neural respiratory drive and respiratory muscle effort, respectively.

Results
Dyspnea unpleasantness was significantly greater with the FM at the highest equivalent submaximal work rate achieved by a given participant in both conditions (iso-work; 5.9 ± 1.7 vs 3.9 ± 2.9 Borg 0–10 units, P = 0.007) and at peak exercise (7.8 ± 2.1 vs 5.9 ± 3.4 Borg 0–10 units, P = 0.01) with no differences in dyspnea intensity ratings throughout exercise compared with control. There were significant increases in the sensory quality of “smothering/air hunger” (P = 0.01) and the emotional response of “anxiousness” (P = 0.04) in the FM condition. There were significant increases in diaphragmatic EMG and esophageal pressure at select submaximal work rates, but no differences in heart rate, pulse oximetry–derived arterial oxygen saturation, or breathing frequency throughout exercise with FMs compared with control. FMs significantly reduced peak work rate and exercise duration (both P = 0.02).

Conclusions
FMs negatively impact the affective domain of dyspnea and increase neural respiratory drive and respiratory muscle effort during exercise, although the impact on other cardiorespiratory responses are minimal.
Monitoring of awake bruxism by intelligent app
Authors: Velásquez Ron Byron , Mosquera Cisneros Verónica , Pazmiño Troncoso Pamela , Rodríguez Tates María , Alvares Lalvay Eddy , Chauca Bajaña Luis , Ordoñez Balladares Andrea 
Published: December 2022

Background. Bruxism is a topic of much controversy and is continually debated in the field of dentistry due to the multifaceted clinical relationship that results in painful conditions and consequences to patients. The aim of this review was to determine the effectiveness of a smartphones app in monitoring awake bruxism. Methods. PROSPERO (registration number: CRD42021271190). The eligibility criteria were as followed: observational studies, case–control studies, studies that reported odds ratios, and studies on awake bruxism. The following keywords were searched: [smartphones apps] AND [apps] AND [awake bruxism], OR [sleep bruxism], OR [sleep hygiene], OR [parasomnias], AND [habits]. Results. All the included studies found that the use of the smartphone app allows controlled awake bruxism monitoring. The results also show that the slepp bruxism and awake bruxism are interactive, having negative synergism and substantially increasing the risks of temporomandibular joint pain and temporomandibular disorders. Discussion. In the awake bruxims it was possible to identify 70% symptoms through the different frequencies of behavior provided by the App, within the present technological tools have become daily in young and adult population. The app is effective and easy to use by patients, effectively limiting biases the time of evaluation.
Evaluation of left ventricular systolic function in children with sickle cell anemia: contribution of 2D strain
Authors: Chenik Sarra , Noamen Aymen , Bouslimi Abyr , Mahfoudhi Houaida , Hannachi Sadok , Barakizou Hager , Mejri Islam , Znegui Tasnim , Fehri Wafa 
Published: December 2022

Background: Children with sickle cell anemia (SCA) are at an increased risk of cardiovascular complications. The aim of this study was to assess the role of speckle tracking echocardiography in detecting subclinical myocardial damage in children with SCA. Methods: A cross-sectional case–control study was conducted at the echocardiography laboratory of the military hospital of Tunis between July and December 2018. Thirty patients with SCA were included. A control(C) group including 30 normally developing children was selected and matched to the SCA group by sex and age. We compared between the two groups: conventional echocardiographic parameters including cardiac output, left ventricular ejection fraction (LVEF), thickness and the global longitudinal strain (GLS). The echocardiographic measurements were indexed according to body surface area. The left ventricular (LV) GLS association with clinical characteristics and echocardiographic parameters were also evaluated. Results: Patients and controls were matched for age and sex: the mean age was (11± 2years) in SCA group versus (12± 1 years) in C group with a sex ratio of (1.31 versus 1.27, respectively). Body surface area was comparable. LV hypertrophy and dilation were revealed in the SCA group, whereas measurements were normal in the C group. No significant differences were observed for cardiac output (p=0.4). LVEF were preserved in both groups. However, two-dimensional (2D) LVGLS was impaired in 46% of SCA group (n=14) with mean value of (-21%±3.07 vs -25%±2.98; p<0.01).In SCA group, impaired LVGLS was significantly associated with LV mass (r = – 0.399, p<0.01), LV tele diastolic diameter(r= -0.419, p<0.01) and left atrial volume (r= - 0.399, p< 0.04). In multivariate analysis, LV mass was the only independent factor. Conclusions: In the present study, LVGLS measurement revealed subclinical LV systolic impairment in patients with SCA. Therefore, 2D strain could be beneficial to detect the natural history of LV dysfunction in SCA.
Interrogation of an ovine serum peptide spectral library to annotate ambiguous clinicopathological biomarkers using data-independent acquisition
Authors: Chemonges Saul 
Published: December 2022

Background: The use of data-independent data acquisition mass spectrometry (DIA-MS) on biological samples from domestic animals is still uncommon. Here, sequential window acquisition of all theoretical mass spectra (SWATH-MS) – a variant of DIA-MS was used to analyse serum peptides of healthy sheep as compared with serum of sick sheep by interrogating a novel peptide spectral library (PSL). This approach enabled the detection and annotation of a wide range of proteins, than conventional clinical pathology protein assays. Methods: Serum samples from healthy sheep were obtained from a commercial source and normalised to represent a healthy sheep proteome background and then compared with serum samples of sheep suffering from a range of naturally-acquired illnesses submitted to The University of Queensland, Australia. Purified tryptic peptides were subjected to liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) on a quadrupole time-of-flight instrument (TripleTOF 5600+, SCIEX) set in a cyclic data-independent acquisition (DIA) mode using a generic (SWATH™, SCIEX) acquisition method. Data were processed using PeakView® v2.2 software with SWATH™ Acquisition MicroApp 2.0 (SCIEX) and MarkerView™ v1.3 software (SCIEX) pipeline to generate protein lists for downstream gene ontology annotation and pathway analysis of identified proteins. Results: There were distinct differences in peptide chromatographic features of sick sheep samples compared to those from healthy sheep. Healthy and sick sheep serum samples yielded 335 and 236 protein identifications (IDs), respectively. There were 96 protein IDs unique to sick sheep serum. A total of 431 protein IDs were annotated by combining healthy control and sick sheep protein IDs. Conclusions: SWATH analysis successfully aided in the detection some established clinicopathological serum biochemical analytes. This approach enabled the distinction of protein profiles of sick sheep samples from a healthy control sample, thereby providing a promising future perspective for the application of SWATH analysis in veterinary clinical use.
Towards equality: gender representation at the Royal College of Radiologists’ Annual Scientific Meeting 2014-2021
Authors: Gibson Lorna M. , Wood Kayleigh L. , Wardlaw Joanna M. 
Published: December 2022

Background: Conferences facilitate career advancement, but gender imbalances in public fora may negatively impact both women and men, and society. We aimed to describe the gender distribution of presenters at the UK’s 2014-2021 Royal College of Radiologists’ (RCR) Annual Scientific Meeting. Methods: We extracted data on presenter name, role and session type from meeting programmes. We classified gender as male or female using names, records or personal pronouns, accepting the limitations of these categories. We classified roles by prestige: lead, other (speakers and workshop faculty), proffered paper or poster presenters. We calculated odds ratios (OR) and 95% confidence intervals (CI) for associations between gender and binary outcomes using logistic regression. Results: Women held 1,059 (37.5%) of 2,826 conference roles and presented 9/27 keynotes. Compared to men, women were less likely to hold other roles such as speakers and workshop faculty (OR 0.72 95% CI 0.61-0.83), and more likely to present posters (OR 1.49 95% CI 1.27-1.76). There were 60 male-only and eight women-only multi-presenter sessions. Sessions led by women had higher proportions of women speakers. The odds of roles being held by women increased during online meetings during COVID (OR 1.61, 95% CI 1.36-1.91). Conclusion: The proportion of women presenters and keynote speakers reflects that of RCR membership, but not of the patient population. Disadvantage starts from the earliest career stages, prejudicing career opportunities. Efforts to improve inclusion and diversity are needed; focusing on lead roles and hybrid online/in-person formats may accelerate change.