bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disordered-to-ordered transitions in assembly factors allow the Complex II catalytic subunit to switch binding partners
Authors: Sharma Pankaj , Maklashina Elena , Voehler Markus , Balintova Sona , Dvorakova Sarka , Kraus Michal , Vanova Katerina Hadrava , Nahacka Zuzana , Zobalova Renata , Boukalova Stepana , Mracek Tomas , Ghayee Hans , Pacak Karel , Rohlena Jakub , Neuzil Jiri , Cecchini Gary , Iverson T 
Published: December 2022

Abstract
Mitochondrial Complex II (CII) activity supports multiple types of respiration and connects metabolic state with succinate signaling. It is therefore unsurprising that CII activity has been linked to biological phenomena that require crosstalk between metabolism and signaling, including neurodegeneration, metabolic shifts in cancer, immune activation, and ischemia reperfusion injury. In each of these processes, CII activity may be regulated at the level of assembly, with the disassembled SDHA subunit stored as metastable species with one or more assembly factors. However, both the exact molecular nature of these species and the mechanism of release of SDHA from these metastable species is unclear. In this work, we identified that transfer of SDHA into CII proceeds through at least three metastable intermediates, we assigned the sequence of appearance of these intermediates, and we determined structures for two of these. A complex between SDHA and one of its assembly factors, succinate dehydrogenase assembly factor 2 (SDHAF2), is likely the main alternative species that accumulates in cells. A second assembly factor, succinate dehydrogenase assembly factor 4 (SDHAF4), must displace SDHAF2 for assembly to proceed. Changes in intrinsic disorder in both SDHAF2 and SDHAF4 are required to release SDHA from the metastable complexes. Evaluation of unrelated biological complexes suggests that changes in disorder may guide post-folding biogenesis during both self-assembly and assisted assembly.
A crystalline stibinidene with a triplet ground state
Authors: Wu Mengyuan , Li Hao , Chen Wang , Wang Dongmin , He Yuhao , Ye Shengfa , Tan Gengwen 
Published: December 2022

Abstract
Pnictinidenes (R-E; E = P, As, Sb or Bi) are long-sought reactive species, which are isoelectronic to carbenes. They feature either a singlet or triplet ground state depending on the electronic property of the substituents. Although isolable singlet carbenes and fairly stable triplet carbenes are well-documented, triplet pnictinidenes isolable under standard laboratory conditions remain elusive. Herein we report the isolation, characterizations and reactivity studies of a free stibinidene supported by a hydrindacene ligand. X-ray crystallographic analysis shows that it contains a one-coordinate antimony atom featuring a Sb–C single bond. Magnetometric investigations, in conjunction with highly correlated wavefunction based ab initio calculations unequivocally demonstrate that the stibinidene features a triplet electronic ground state.
A comparative evaluation of serum lipid profile and blood pressure measurements in adult hypertensive patients consuming Moringa stenopetala herbal tea and non-consuming groups as control, South East Ethiopia, 2021
Authors: Gezahegn Bereket , Regassa Tadele , Solomon Damtew , Derbew Meseret , Malik Tabarak , Ayelgn Tiget , Shibabaw Tewodros 
Published: December 2022

Abstract
Background
Noncommunicable diseases, such as cardiovascular diseases, continue to be the leading cause of morbidity and mortality around the world. Hypertension and dyslipidemia appear to be the two most important modifiable risk factors contributing to the global rise in cardiovascular disease. Several plant preparations are currently being touted as having benefits for such cardiovascular risk factors. African moringa, Moringa stenopetala, is one of these plants used in Ethiopia. Therefore, this study aimed to assess and compare the serum lipid profiles and blood pressure measurements of Moringa stenopetala herbal tea drinkers and non-drinker hypertensive patients attending chronic care follow-up at Bale zone hospitals.
Methods
A comparative cross-sectional study, involving 128 blood samples (64 each of Moringa stenopetala herbal tea drinkers and nondrinkers), was conducted on hypertensive patients who were on chronic care follow-up from July 1 to August 30, 2021. A face-to-face interview was used to collect data, and serum LDL-c, HDL-c, TG, and TC levels were measured using a clinical chemistry analyzer. Data were analyzed using SPSS version 25, Chi-square (X2) was used to compare the relationship between categorical variables, and an independent sample t-test and one-way ANOVA were used to compare the mean of the groups. Generalized linear regression was used to study the association between continuous variables and Moringa stenopetala herbal tea consumption groups. The statistical significance was declared at p < 0.05.
Results
Moringa stenopetala herbal tea drinkers have showed statistically significant lower values than non-drinkers for LDL-c (p < 0.001), TG (p = 0.001), TC (p < 0.001), TC/HDL-c ratio (p < 0.001), and LDL-c/ HDL-c ratio (p < 0.001). The mean SBP, DBP, and MAP were significantly lower among the Moringa stenopetala tea drinkers group (p < 0.001, p = 0.002, and p < 0.001 respectively).
Conclusions
Interestingly, Moringa stenopetala herbal tea consumption has a significant health benefit by lowering blood pressure, LDL-c, TG, TC, and the ratios TC/HDL-c and LDL-c/HDL-c, which have the potential risk of developing CVDs.
Tumor to liver maximum standardized uptake value ratio of FDG-PET/CT parametersBBpredicts tumor treatment response and survival of stage III non-small cell lung cancer
Authors: Zhang Pengfei , Chen Wei , Zhao Kewei , Qiu Xiaowen , Li Tao , Zhu Xingzhuang , Sun Peng , Wang Chunsheng , Song Yipeng 
Published: December 2022

Abstract
Bacground: To assess the predictive values of primary tumor FDG uptake for patients with inoperable stage III non-small cell lung cancer (NSCLC) after concurrent chemoradiotherapy (CCRT).
Methods: A total of 107 patients with diagnosis of stage III NSCLC and CCRT were enrolled in this study. The tumor maximum uptake value (SUVmax) was standardized by calculating several ratios between tumor and each background tissues. The receiver operating characteristics curve (ROC) was used to compare the predictive power of prognostic models. The tumor objective response rate (ORR) and overall survival (OS) were compared and analyzed by the Kaplan–Meier method and univariate and multivariate Cox regression models.
Results: The areas under ROC curve (AUCs) ranged from 0.72 to 0,81 among these tumor SUVmax and standardized SUVmax ratios, and the tumor SUVmax and tumor SUVmax-to-liver SUVmean ratio (TLMR) were more predictive of ORR (AUC=0.81; 95% CI, 0.73-0.88 for tumor SUVmax and AUC=0.84; 95%CI, 0.76-0.91 for TLMR) than any of other SUVmax ratios. The patients with lower tumor SUVmax, SUVmean and SUVmax ratios had a significantly better OS than those with their corresponding higher ones. Moreover, both univariate and multivariable analyses revealed that TLMR was significantly associated with better ORR and OS after adjustment with other prognostic variables.
Conclusions: TLMR, a standardized tumor SUVmax, was an independent prognostic predictor for tumor ORR and OS of patients with stage III NSCLC after CCRT.
A novel Weighted Adaptive Aquila Optimizer technique for solving the Optimal Reactive Power Dispatch problem
Authors: Das Tanmay , Roy Ranjit , Mandal Kamal Krishna 
Published: December 2022

Abstract
The major problems in the field of power system engineering can be mostly solved with the help of the ORPD problem. Many recently developed optimization techniques have been implemented in this area of power system to optimize the objective function of minimum power loss, and determine its optimal solution leading to a more efficient and secured system. In this paper, a novel Weighted Adaptive Aquila Optimizer (WAAO) has been proposed to solve the highly complex and non-linear problem of Optimal Reactive Power Dispatch (ORPD). The Aquila optimizer (AO) has been modified with the addition of the unique search feature to develop an algorithm with the capability of optimizing any optimization technique faster and better compared to many other efficient techniques including AO technique. Here, the proposed algorithm has been tested on IEEE 30, 57 and 118 bus systems to minimize the power loss objective function of the ORPD problem, and obtain the optimal solutions to the control variables. The results obtained showed a significant improvement in terms of power saving by minimizing the power loss to a large margin for all the three mentioned test cases, which has not yet been reported earlier in the literature. The detailed study in this work proved that the WAAO has better optimization capability compared to AO and many other well-established techniques in solving the problem of ORPD.
Bioinformatics molecular analysis of prognostic-related genes and drug sensitivity and resistance in head and neck squamous cell carcinomas
Authors: Saleh Raed Obaid , Mustafa Yasser Fakri , Abdullaev Bekhzod , Jalil Abduladheem Turki , Hammid Ali Thaeer , Abid Mohammed Kadhem , Samarghnadian Saeed , Hashemi Mehrdad , Mirzaei Sepideh 
Published: December 2022

Abstract
One of the most heterogeneous collections of malignancies probably is head and neck cancers that in this study, we focus on the head and neck squamous cell carcinomas (HNSCCs). The aim of this study was to recognize the prognosis-related genes and their pathways related to HNSCC and to further analyze its molecular mechanism and drug sensitivity and resistance. Furthermore, the correlation between the expression of candidate genes with immune infiltration and IC50 of some drugs was evaluated. The cancer genome atlas (TCGA) HNSCC was utilized for differential expression analyzing. Differentially expressed genes (DEGs) were indicated by R software. By applying univariate and multivariate Cox regression analysis, and using prognostic risk model, we identified the key prognostic genes in HNSCC based on PPI network, and enrichment analysis of DEGs was performed by Gene Set Enrichment Analysis (GSEA) and Enrichr database. The Tumor Immune Estimation Resource (TIMER) was used to assess the relationship between selected genes and immune infiltration in cancer. Finally, the CCLE and GDSC were used to assess the correlation between the level of selected genes with drug resistance and sensitivity. Our results indicated that four hub genes including NUF2, BUB1, TPX2, and DLGAP5 were selected as poor prognostic genes in HNSCC. Our findings revealed that drugs like Glucosamine, Ribavirin and Tocetrapib could lower the expression of selected genes in HNSCC. In doing so, NUF2, BUB1, DLGAP5, TPX2 were identified as hub-related prognostic genes associated with patients’ survival, drug resistance and sensitivity, and could be effective in the cancer progression.
Lipid extraction and analysis of microalgae strain Pectinodesmus PHM3 for biodiesel production
Authors: Kiyani Dania Akram , Maryam Shafia , Amina Sundus Jabeen , Ahmad Abdullah , Chattha Muhammad Waqas Alam , Janjua Hussnain Ahmed 
Published: December 2022

Abstract
The current study is focused on the lipid extract of
microalgae; Pectinodesmus strain HM3 (PHM3) and its
general analysis in terms of chemical contents. Combinations of
both chemical and mechanistic approaches were applied to obtain the
maximum yield of lipids which was recorded to be 23% per gram
through continuous agitation using Folch solution. The extraction
methods used in this study included: Bligh and Dyers method,
Continuous agitation method, Extraction using Soxhlet and Acid base
extraction method. Lipid quantification of ethanol and Folch
solution lipid extract was performed through gravimetric methods
and qualification was done through Fourier Transmission Infrared
Spectroscopy (FTIR) and Gas Chromatography-mass spectrometry
(GC-MS). Phytochemical analysis identified other compounds in
ethanol extract and the results confirmed the presence of steroids,
coumarins, tannins, phenols and carbohydrates. Transesterification
of lipids showed 7% per gram dry weight yield of
Pectinodesmus PHM3. GC-MS studies of extracted biodiesel
suggested that 72% of biofuels was in the form of dipropyl ether,
ethyl butyl ethers, methyl butyl ether and propyl butyl ether.
Lipid processing of acid-base extract showed that oily nature of
lipid shifted to a more precipitated form which is a common
observation when mixture of lipids is converted to
phosphatides.
Phenotypic aging mediates the association between blood cadmium and depression: A population-based study
Authors: Wu Yudong , Wu Qing , Pan Rubing , Yi Weizhuo , Li Yuxuan , Jin Xiaoyu , Liang Yunfeng , Mei Lu , Yan Shuangshuang , Sun Xiaoni , Qin Wei , Song Jian , Cheng Jian , Su Hong 
Published: December 2022

Abstract
Background
Depression is a serious public health problem today, especially in middle-aged and older adults. Although the etiology of the disease has not been fully elucidated, environmental factors are increasingly not negligible. Cadmium is widely used in industrial production. The general population may be chronically exposed to low doses of cadmium. This study aimed to investigate the association between blood cadmium and depression and to explore the mediating role of aging indicators in this process.
Methods
We conducted a cross-sectional study on blood cadmium (N = 7195, age ≥ 20 years) using data from the 2007–2010 National Health and Nutrition Examination Survey (NHANES). Aging indicators (biological and phenotypic age) are calculated by combining multiple biochemical and/ or functional indicators. To determine the relationship between blood cadmium concentrations and depressive symptoms, we used weighted multivariate logistic regression and restricted cubic spline functions and employed mediation analysis to explore the possible mediating effects of aging indicators in the process.
Results
We found a significant positive association between blood cadmium and depression with an OR and 95% CI: 1.22 (1.04,1.43). Restricted cubic spline analysis found a linear positive association between blood cadmium and depression. In the fully covariate-adjusted model, we found a positive association between blood cadmium and biological age and phenotypic age with β and 95% CI: 1.02 (0.65, 1.39) and 2.35 (1.70, 3.01), respectively. In the mediation analysis, we found that phenotypic age mediated 21.32% of the association between blood cadmium and depression.
Conclusion
These results suggest that even exposure to low doses of cadmium can increase the risk of depression and that this process may be mediated by phenotypic aging.
Topologically protected phase singularities in TMDCs monolayers
Authors: Ma Guoteng , Shen Wanfu , Sanchez Soy , Yu Yu , Wang Han , Sun Lidong , Wang Xinran , Hu Chunguang 
Published: December 2022

Abstract
The non-trivial and rigorous Heaviside phase jump behavior of phase singularities (PSs) empower exotic topological modes and widely divergent nature compared to neighboring points, which has attracted great attention in condensed matter physics as well as applications in photonics and ultrasensitive sensors. Here we demonstrate the universal existence of a new family of topologically protected PSs generated from single atom layers. We obtain the PSs by coating the transition metal dichalcogenides (TMDCs) monolayers on a semi-infinite light non-adsorption substrate without surface plasmon effect or assisted resonators, which exploits the benefits of both excitons dominated enhancement and peculiarities of the singular phase. We show that refractive indices matched transparent substrate enables TMDCs monolayers exhibit topologically protected zero reflection yielding to ideal Heaviside π-phase jump accompanied by strong light-matter interactions, which can be utilized to radically improve the sensitivity of sensors based on the uniquely appeared exciton resonances in a single atomic layer. By using the TMDCs monolayers-based PSs for refractive index bio-sensors, we demonstrate its superior phase sensitivity at a level of 10^4 degrees per refractive index unit and detection of bioactive bacteria, respectively, which is comparable to the cutting-edge surface plasmon and Fabry-Perot resonance sensors. Our proof-concept results offer experimental and theoretical insights into an entirely new single atomic playground for flat singular optics and label-free biosensing technologies.
Chemoproteomic target deconvolution of lipoic acid reveals Histone Deacetylases as the main targets
Authors: Kuster Bernhard , Lechner Severin , Steimbach Raphael , Wang Longlong , Matthias Patrick , Miller Aubry , Medard Guillaume 
Published: December 2022

Abstract
Lipoic acid is an essential enzyme cofactor in central metabolic pathways. Due to its claimed antioxidant properties, racemic (R/S)-lipoic acid is used as a food supplement, but is also investigated as a pharmaceutical in over 180 clinical trials covering a broad range of diseases. Moreover, (R/S)-lipoic acid is an approved drug for the treatment of diabetic neuropathy. However, its mechanism of action remains elusive. Here, we performed chemoproteomics-aided target deconvolution of lipoic acid and its active close analogue lipoamide. We find that histone deacetylases HDAC1, HDAC2, HDAC3, HDAC6, HDAC8 and HDAC10 are molecular targets of the reduced form of lipoic acid and lipoamide. Importantly, only the naturally occurring R-enantiomer inhibits HDACs at physiologically relevant concentrations and leads to hyperacetylation of HDAC substrates. The inhibition of HDACs by (R)-lipoic acid and lipoamide explains why both compounds prevent stress granule formation in cells and may also provide a molecular rationale for many other phenotypic effects elicited by lipoic acid.
Psychometric and Clinical Evaluation of Schizophrenia Remission Criteria in Swedish Outpatients with Psychotic Disorders
Authors: Sakinyte Karolina , Holmberg Christopher 
Published: December 2022

Abstract
Background: Psychotic disorders including schizophrenia have debilitating effects on health and functioning. In recent years, symptomatic remission has emerged as an increasingly viable treatment goal. The Remission in Schizophrenia Working Group criteria (RSWG-cr) are based on 8 items from the Positive and Negative Syndrome Scale (PANSS-8). The criteria, frequently used in clinical and research settings, lack Swedish validation.
Objectives: To evaluate the psychometric properties of PANSS-8, and to examine the clinical validity of RSWG-cr in a Swedish outpatient population.
Methods: Cross-sectional register data was collected between 2016–2019 from outpatient psychosis clinics in Gothenburg, Sweden. Firstly, confirmative and exploratory factor analyses were performed on PANSS-8 data (n=1744) to assess the scale and subscales’ psychometric properties. Internal reliability was evaluated using Cronbach’s alpha. Second, 649 patients with sociodemographic and clinical data registered within the same 7-day period were classified according to RSWG-cr. Remitted and non-remitted patients were compared in terms of clinical and sociodemographic characteristics. Depending on the nature of data, parametric (e.g., t-tests) and non-parametric (e.g., Mann-Whitney U test) analyses were used. Lastly, odds ratios (OR) were estimated using binary logistic regression to assess the independent contribution of each variable to remission status.
Results: PANSS-8 showed good internal reliability (alpha = 0.85). A three-dimensional model reflecting psychoticism, disorganization, and negative symptoms showed good fit in confirmatory factor analysis. Of 649 patients, 356 (55%) were in remission according to RSWG-cr. Remitted patients were significantly more likely to live independently, have their own income, be non-smokers, not currently on antipsychotic medication, and have a recent health interview and physical examination registered (all p < 0.01). Binary logistic regression found increased likelihood of remission among patients living independently (OR = 1.98), having their own income (OR = 1.89), obese patients (OR = 1.61), and patients with a recent physical examination (OR = 1.56), and decreased likelihood among patients on antipsychotic medication (OR = 0.41).
Conclusions: PANSS-8 as a whole was internally reliable, and remission according to RSWG-cr was associated with significant clinical variables in a naturalistic Swedish outpatient sample. Future longitudinal studies should be conducted to better assess the direction of these relationships.
Land surface temperature in an arid city: assessing spatio-temporal changes
Authors: CAMPOS VALERIA E. , GATICA GABRIEL , ANDINO NATALIA , MALDONADO VIVIANA N. FERNÁNDEZ , CARDÚS ADRIANA 
Published: December 2022

Abstract
In a city located in a desert environment (Tulum Valley, Argentina) we proposed to assess in the coldest and warmest periods (1) the urban cold/heat island (UCI/UHI) phenomenon; 2) which driving factors currently affect the Land Surface Temperature (LST). In the study area, we selected 50 points for the urban class and 49 points for the rural class. The LST data was obtained from Landsat 5 TM y Landsat 8 OLI/TIRS, for 1988, 2000, 2010, and 2021 years. As driver factors, we assessed the median (med) and standard deviation (sd) of NDVI (Normalized Difference Vegetation Index), NDBI (Normalized Differences Built-up Index), and BI (Brightness Index). The Tulum Valley behaved like a UCI during almost all studied years, except for 2010 and 2021. Probably because the city was undergoing reconstruction after a major earthquake. In the urban class, the LST were affected positively by NDVImed during the cold period, while in the rural class the LST were explained by a negative relationship with NDVImed, and positive with NDBImed. In the urban class during the warm periods, the LST were affected negatively by BIsd, BImed, NDVImed, and NDVIsd, which leads to a decrease in LST. Contrarily, an increase of NDBIsd produces an increment of LST. The only driver for LST in the rural class was NDBImed, which had a positive effect. Different drivers affected the LST behavior in Tulum Valley, moreover, these drivers explained more variability in rural than in urban class.
On thresholds for controlling negative particle (PM 2.5 ) readings in air quality reporting
Authors: Jiang Ningbo , Akter Rinat , Ross Glenn , White Stephen , Kirkwood John , Gunashanhar Gunaratnam , Thompson Scott , Riley Matthew , Azzi Merched 
Published: December 2022

AbstractAmbient PM2.5(particles less than 2.5 µm in diameter) is monitored in many countries including Australia. Occasionally PM2.5instruments may report negative measurements, although in realty the ambient air can never contain negative amounts of particles. Some negative readings are caused by instrument faults or procedural errors, thus can be simply invalided from air quality reporting. There are occasions, however, when negative readings occur due to other factors including technological or procedural limitations. Treatment of such negative data requires consideration of factors such as measurement uncertainty, instrument noise and risk for significant bias in air quality reporting. There is very limited documentation on handling negative PM2.5data in the literature. This paper demonstrates how a threshold is determined for controllingnegative hourlyPM2.5readings in the New South Wales (NSW) air quality data system. The investigation involved a review of thresholds used in different data systems and an assessment of instrument measurement uncertainties, zero air test data, and impacts on key reporting statistics when applying different thresholds to historical datasets. The results show that a threshold of -10.0 µg/m3appears sufficient for the NSW data system. This work has served to be part of local evidence for formulating the recently published Australian Standard, AS 3580.19.2020 – Method 19: Ambient air quality data validation and reporting. The analysis can be useful for air monitoring in other Australian jurisdictions or wider context.