bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictors of hepatic steatosis in living liver donors
Authors: Minina M. G. , Voronov D. V. , Nevredimov A. A. , Tenchurina E. A. 
Published: December 2022

Fatty liver disease (steatosis) is considered a risk factor in donor liver transplantation (LT). Macrosteatosis (>50%) is associated with primary graft dysfunction and may reduce long-term recipient survival.Objective: to identify predictors of macrovesicular steatosis (>50%) by analyzing donor characteristics.Materials and methods. The retrospective study included 525 potential liver donors between January 1, 2019 and December 31, 2020. Clinical and morphological characteristics of donors were studied using logistic regression and receiver operating characteristic (ROC) analysis. Threshold values of parameters demonstrating statistical significance in multivariate analysis as predictors of >50% hepatic steatosis were obtained by ROC analysis based on calculation of the optimal cutoff point.Results. Diabetes mellitus (DM), cause of donor’s death (traumatic brain injury), alanine transaminase (ALT) >90 units/L and aspartate transaminase (AST) >110 units/L were predictors of >50% steatosis, revealed by time-zero biopsy in the donor. Almost identical sensitivity and specificity indicators were determined in ROC analysis for liver enzymes – ALT and AST – which were 69.1 and 80.6; 72.2 and 81.1, respectively. Given the obtained values, we can say that with elevated levels of liver enzymes in the donor’s blood, there is a high degree of probability of liver parenchymal damage, but low sensitivity indicates possible multifactoriality of liver damage, and fatty liver disease may be one of the factors, but there may also be no damage to the liver parenchyma. At the same time, the rather high specificity revealed in ROC analysis for liver enzymes is a reliable sign of the absence of fatty liver disease at enzyme values less than the threshold.Conclusion. The thresholds established for ALT and AST and their corresponding levels of sensitivity and specificity indicate that these parameters have a relatively low predictive level in the context of the presence of severe fatty liver disease in a donor. This allows, nevertheless, to use models built on their basis as screening models in the primary evaluation of liver donors.
Apoptotic bone marrow-derived mononuclear cells accelerate liver regeneration after extended resection
Authors: Onishchenko N. A. , Nikolskaya A. O. , Gonikova Z. Z. , Kirsanova L. A. , Shagidulin M. Yu. , Sevastianov V. I. 
Published: December 2022

Objective: to compare the efficiency of regenerative processes in the liver using apoptotic bone marrow-derived mononuclear cells (BMMCs) and intact BMMCs from healthy animals on an extended liver resection (ELR) model.Materials and methods. Male Wistar rats (n = 77) with an ELR model (70–75%) were divided into 3 groups: group 1 (control with a single intraperitoneal injection of saline), group 2 (single intraperitoneal injection of unsorted intact BMMCs at a dose of 30–35 × 106, and group 3 (single intraperitoneal injection of apoptotic BMMCs at the same dose). Restoration of biochemical parameters of liver function and mass, as well as the emerging microstructural changes in hepatocytes in histological preparations, were monitored by assessing hepatocyte mitotic activity (MA) during the first 7–10 days after ELR.Results. It was found that in groups 2 and 3, as compared with group 1, there was no death after ELR modeling, and that the biochemical parameters of liver function normalized more rapidly (at days 10–14). Hepatocyte MA in group 3 sharply increased as early as on day 1, and mitotic index (MI) averaged 14‰, reaching 20.9‰ in some experiments; MI in the control group remained at the baseline by this time, while in group 2, MI was only 3.2‰. In group 3, liver mass recovered more rapidly after ELR to baseline values already at days 8–10, whereas the recovery was at day 12–14 and day 17–20 in group 2 and group 1, respectively. It was suggested that the more pronounced increase in the efficiency of regenerative processes in the liver after ELR in group 3 after using apoptotic BMMCs was due to the release from these cells of a large spectrum of formed paracrine factors, including various classes of RNA molecules involved in the regeneration process.Conclusion. Apoptotic BMMNCs have a more effective adaptive and regulatory potential than intact BMMCs because reorganizations are rapidly formed in the damaged liver cells, providing an early and more powerful activation of the targeted regenerative program.
Training in biobanking in the context of modern medical education
Authors: Sokolova T. S. , Kamenskikh E. M. , Boguta D. V. , Bahareva Yu. O. , Fedorova O. S. 
Published: December 2022

The development of biomedical research based on predictive, preventive and personalized medicine has served as a challenge to the formation and rapid development of a novel interdisciplinary scientific area — biobanking, the main goal of which is the long-term proper storage of biological samples and related data for use in scientific and clinical research. Qualified personnel, along with the creation and development of biobank infrastructure, provide high-quality results required for biomedicine. Education and training must reflect the changing scope of knowledge and adapt to biobanking challenges. In this regard, there is an increasing need to develop and implement educational programs for staff working in and managing biobanks, as well as researchers, doctors and students who are not familiar with this area, but plan research using bioresources. The aim of this review is to present an analysis of the main biobanking directions and training programs in the world and in Russia in order to assess the existing problems and needs.
Biobank as a source of samples for determination of concentration reference ranges for cytokines, chemokines and growth factors circulating in the blood
Authors: Sushentseva N. N. , Popov O. S. , Apalko S. V. , Urazov S. P. , Anisenkova A. Yu. , Minochkin A. K. , Agafyina A. S. , Shcherbak S. G. 
Published: December 2022

Aim. To determine the reference ranges of concentrations of 38 cytokines, chemokines and growth factors, as well as to measure the content of these analytes in patients with neurodegenerative and cardiovascular diseases (CVDs) using biomaterial from the biobank repository.Material and methods. The study included 303 serum and plasma samples from 281 healthy donors, 242 samples from 224 patients with neurodegenerative diseases, and 164 samples from 152 patients with CVDs from the biobank of the of St. Petersburg City Hospital № 40. In all samples, the concentration of 38 cytokines, chemokines, and growth factors was determined by multiplex immunofluorescence assay.Results. Based on the measured concentrations in the group of healthy donors, non-parametric 95% reference ranges with 90% confidence intervals were calculated. For the majority of analytes, no sex and age differences were observed. In donors >65 years of age, the concentration of macrophage inflammatory protein-1-α was reduced and the levels of interleukin-8 and the chemokine interferon-inducible protein 10 were increased. Young donors (18-35 years) had lower levels of tumor necrosis factor-α. In groups with neurodegenerative and cardiovascular diseases, multiple deviations from the calculated reference values were found.Conclusion. Certain reference intervals are intended to evaluate the concentrations of cytokines, chemokines and growth factors determined in blood serum or plasma using Human Cytokine/Chemokine Magnetic Bead Panel 1 reagents (Merck, Millipore) on the MAGPIX system and do not imply a direct transfer to other analytical methods.
Bioresource collection of biosamples from pregnant women at different gestational ages for the search for early biomarkers of pregnancy complications and potential for its use in research
Authors: Pachulia O. V. , Illarionov R. A. , Vashukova E. S. , Maltseva A. R. , Postnikova T. B. , Nasykhova Yu. A. , Bespalova O. N. , Glotov A. S. 
Published: December 2022

Aim. Clinical and anamnestic characterization of the bioresource collection of biosamples from pregnant women at different gestational ages.Material and methods. Preparation of biosamples of various biomaterial types for long-term storage; static analysis of clinical and anamnestic characteristics of women whose samples are included in the collection.Results. As of September 1, 2022, the collection consists of 16625 biosamples of various biomaterial types from 355 pregnant women. The clinical and anamnestic characteristics of women, samples of which are presented in the collection, are presented.Conclusion. The resulting high-quality biosamples can be used in various basic and applied research to study both the physiological and pathological course of pregnancy. Particularly promising is the possibility of searching for the earliest biomarkers of gestational complications before the onset of obstetric complications. In this regard, it is important to have complete clinical and anamnestic data on biosamples of the collection in order to expand the possibilities of its use and improve the quality of ongoing research.
Risk management in biobanking
Authors: Borisova A. L. , Pokrovskaya M. S. , Meshkov A. N. , Kontsevaya A. V. , Drapkina O. M. 
Published: December 2022

Risk management is a key aspect of the organization and management of biobanks, which is part of the overall quality management system aimed at early detection, analysis and minimization of events, that can lead to negative consequences for the biobank, as well as affect the quality of biosamples and related data. The article presents the biobanking risk classification with the description of each category.Aim. To develop and implement the methodology for identification, analysis, evaluation and development of risk management measures for the biobanking process in the biobank of the National Medical Research Center for Therapy and Preventive Medicine.Material and methods. We present the methodology of the risk management process developed on the basis of the literary data, world experience and experience of the biobank of the National Medical Research Center for Therapy and Preventive Medicine.Results. The biobanking risk management procedure was developed and implemented in the biobank of the National Medical Research Center for Therapy and Preventive Medicine in 2020. The work carried out made it possible to identify, analyze and evaluate a wide range of potential negative events and actions that could lead to biobank damage, both in the form of financial losses and ethical and technical issues related to the biobanking process. A significant reduction in the frequency of emergency events and the high stability of the biobank operation under the influence of various external factors prove the effectiveness of the approach used.Conclusion. The creation and maintenance of a risk management system in the biobank allows, in combination with other measures, to ensure the safety and high quality of the procedures for collecting, processing and long-term storage of biomaterial and related data by creating an environment that rules out or minimizes the impact of various risks.
Development of a biobank in the structure of scientific and diagnostic and treatment institutions and prospects for interregional integration
Authors: Kalinin R. S. , Goleva O. V. , Illarionov R. A. , Tsai V. V. , Mukomolova A. L. , Konstantinova Yu. E. , Markin I. V. , Krylov A. V. , Rogozina N. V. , Bekhtereva M. K. , Tyan M. S. , Orlova E. D. , Donnikov M. Yu. , Kovalenko L. V. , Kolbasin L. N. , Glotov A. S. , Glotov O. S. 
Published: December 2022

The formation of biobanks in the structure of scientific and treatment and diagnostic institutions with prospects for interregional integration is a fundamental link in monitoring and predicting diseases of various origins, creating and testing highly effective diagnostics, and developing novel therapeutic agents.Aim. To describe standard operating procedures and principles for the formation of bioresource collections (BRC) in medical institutions with biobanking.Material and methods. The data of scientific and practical biomedical projects using BRC obtained from patients with genetic, multifactorial and infectious diseases in St. Petersburg and Surgut are presented. As of September 2022, the BRC collected on the basis of the Pediatric Research and Clinical Center for Infectious Diseases of the Federal Medical and Biological Agency includes biosamples from 1619 patients, and the BRC collected in the Medical Institute of Surgut State University includes biosamples from 450 patients and healthy individuals of different sex and age. The selection of biosamples from apparently healthy individuals and patients with various diseases can serve as a strategically important resource for future research in terms of etiology, epidemiology, the development of regulatory environment and scales, innovations in the development of diagnostic approaches and treatment of the Russian population.
Approaches to automation of the preanalytical phase of large-scale research in the biobank of the National Medical Research Center for Therapy and Preventive Medicine of the Ministry of Health of Russia
Authors: Pokrovskaya M. S. , Borisova A. L. , Kondratskaya V. A. , Efimova I. A. , Ershova A. I. , Drapkina O. M. 
Published: December 2022

Aim. To create and validate an algorithm for automatic aliquoting of serum and plasma samples for biobanking as part of a large-scale study.Material and methods. Biobank of the National Medical Research Center for Therapy and Preventive Medicine is equipped with a Tecan automated aliquoting system. When compiling the aliquoting program (script), the following parameters were selected: the time spent on spotting one complete cryobox, with a capacity of 96 cryotubes, the optimal number of vacutainers and tips for a single loading of the device. The program was created to receive 12 aliquots of 0,5 ml of blood serum, plasma with ethylenediaminetetraacetic acid and plasma with sodium citrate in cryotubes per 1 ml from eight participants in total from each in one cycle of device loading. Automatic and manual spotting was assessed in terms of the time spent on sample preparation and the quality of the aliquots obtained.Results. A methodology for conducting the preanalytical phase of a large-scale study based on the automation of biosample aliquoting has been developed and validated. We created scripts for aliquoting serum and blood plasma at the automated Tecan Freedom EVO system. An experiment conducted on biomaterial from 64 participants showed, that with an expected flow of 32 participants per day, it took more than 2 hours for manual aliquoting, and for automatic aliquoting (4 launches of the aliquot robot for 24 vacutainers from 8 participants) — less than 1,5 hours with the complete exclusion of human errors.Conclusion. Automated aliquoting has a following number of advantages in comparison with manual: it allows to guarantee standardization and efficiency of sample preparation, reduce the time and increase the accuracy of aliquoting of biomaterial, save space in long-term storage freezers due to the use of smaller cryotubes. The developed algorithm for creating aliquoting programs and calculating the optimal use of consumables can be used in other projects.
Influence of long-term storage of blood serum samples in a biobank for population-based seroepidemiologic studies
Authors: Nozdracheva A. V. , Semenenko Т. A. 
Published: December 2022

Aim. To assess the safety of IgG antibodies during long-term storage of blood serum samples using measles- and rubella-specific antibodies as an example.Material and methods. The study used serum samples from the collection of the Department of Epidemiology of the Gamaleya National Research Center for Epidemiology and Microbiologya, which were tested for measles- or rubella-specific IgG antibodies immediately upon admission to the laboratory, were frozen and stored at -70оC (n=180). The samples were reexamined after 20 months (n=90) and 6 years (n=90).Results. Reexamination after 20-month storage showed a decrease in the mean level of measles- and rubella-specific antibodies by 13,1% (from 0,36 (0,08-1,21) to 0,31 (0,02-1,2) IU/ml) and by 11,8% (from 151,4 (45-235) to 133,5 (72-198) IU/ml), respectively (p<0,05 for all). At the same time, the number of seronegative samples did not change. After a 6-year storage period, the mean level of measles-specific antibodies decreased by 33,8% (p<0,05) from 0,72 (0,4-1,79) to 0,34 (0,18-1,14) IU/ml and only two samples became seronegative. It was shown that the higher the IgG level in native samples, the more pronounced its decrease was during the second examination.Conclusion. Storage of serum samples in a biobank for 20 months at -70оC ensures the safety of measles- and rubella-specific IgG antibodies, and is the basis for the reliability of future studies.
The concept of the national information platform of biobanks of the Russian Federation
Authors: Meshkov A. N. , Yartseva O. Yu. , Borisova A. L. , Pokrovskaya M. S. , Drapkina O. M. 
Published: December 2022

Aim. To create the concept of the national information platform of biobanks of the Russian Federation (NIPB RF).Material and methods. In April 2022, an expert group consisting of 17 representatives of Russian biobanks was formed in National Association of Biobanks and Biobanking Specialists to create the concept of the NIPB RF. An analysis of international practices in this area was carried out. To assess the infrastructure of Russian biobanks in terms of information support for stored biomaterial, experts formed a questionnaire consisting of several blocks of questions. The survey was conducted from April to June 2022 among representatives of Russian biobanks. Descriptive statistics were used to analyze the results of the survey.Results. In total, representatives of 16 biobanks took part in the survey. The storage capacity of biobanks varies from 2000 to 800000 storage units. It is shown that the majority of biobanks (81%) use information systems for entering and storing related data. Biobanks use foreign and Russian software products (53 and 47%, respectively), while a third develop their own information systems. We formulated the NIPB RF concept as an information system designed for consolidating, processing and using data on biosamples for the development of Russian biobanking and improving the efficiency and quality of research.Conclusion. The creation of the NIPB RF will improve the interaction between biobanks and end users and will develop biomedicine in Russia.
How to interpret and use the results of epidemiological studies in healthcare practice. Methodological Rationale
Authors: Shalnova S. A. , Balanova Yu. A. , Vilkov V. G. , Zelenina A. A. , Evstifeeva S. E. , Imaeva A. E. , Kapustina A. V. , Karamnova N. S. , Kotova M. B. , Kutsenko V. A. , Maksimov S. A. , Muromtseva G. A. , Shvabskaya O. B. , Yarovaya E. B. , Drapkina O. M. 
Published: December 2022

The methodological rationale contains a description of epidemiological research methods, provides an analysis of the key concepts of epidemiology (population, sample, risk factor (RF)), in the context of prevention priorities, as well as describes each of the main RFs for noncommunicable diseases (NCDs), including cardiovascular diseases. Emphasis is placed on behavioral and mediated biological RFs, such as smoking and alcohol consumption, poor nutrition (low consumption of vegetables and fruits), physical inactivity, high blood pressure, dyslipidemia and obesity, hyperglycemia and diabetes. The methodological rationale was created with the aim of popularizing epidemiological studies, expanding the scope of its use by clinicians, primary care physicians, specialists from medical prevention centers, providers of preventive measures, as well as decision makers in the healthcare system. The sphere of the implementation of epidemiological data covers both the planning of preventive programs for the modification of risk factors in the population, and monitoring the effectiveness of preventive measures. These materials will also be useful in the development of a population, regional and municipal strategy for the prevention of NCDs and related RFs in Russia.
An Efficient and Reliable Data Transmission Service using Network Coding Algorithms in Peer-to-Peer Network
Authors: Sable Nilesh P. , Rathod Vijay U. , Mahalle Parikshit N. , Railkar Poonam N. 
Published: December 2022

Network coding is a progressive enhancement in natural network routing that increases throughput and reliability for unicast, multicast, and even broadcast transmissions. P2P networks are ideal for implementing network coding algorithms for two reasons: I. A P2P network's topology isn't predetermined. As a result, designing a topology that is compatible with the network coding algorithm is much easier. II. Every peer is an end host in this network. As a result, instead of saving and sending the message, complex network coding operations, such as encoding and decoding, are now easier to perform. The objective of this work is to use the best features of network coding algorithms and properly apply them to P2P networks to create an efficient and reliable data transmission service. The goal of the network coding algorithm is to make better use of network resources and thus increase P2P network capacity. An encoding algorithm that enables an intermediate peer to produce output messages by encoding (that is, computing a function of the data it receives. The decoder's role is to obtain enough encoded packets so that the original information can be recovered. This research work has measured an amount of hypothetical and applied consequences in which the network coding procedure or a variation of it is used to improve performance parameters such as throughput and reliability in P2P network data transmission based on network coding. The comparison of data transmission between network routing and network coding algorithms was the main focus of this paper. According to our simulations, the new network coding systems can reach 15% to 20% upper throughput than supplementary P2P network routing systems.
Policy-aware Distributed and Dynamic Trust based Access Control Scheme for Internet of Things
Authors: Railkar Poonam Ninad , Mahalle Parikshit Narendra , Shinde Gitanjali Rahul , Sable Nilesh P. 
Published: December 2022

The use of smart devices is driving the Internet of Things (IoT) trend today. Day by day IoT helps to support more services like car services, healthcare services, home automation, and security services, weather prediction services, etc, to ease user’s life. Integration of heterogeneous IoT devices and social resources sometimes creates many problems like the privacy of data. To avoid privacy issues, an appropriate access control mechanism is required to check authorized and trusted devices, so that only valid devices can access the data which is only required. In the sequel, this paper presents implementation of distributed and dynamic trust based access control mechanism (DDTAC) for secure machine to machine communication or distributed IoT environment. Novelty of this mechanism is that, it uses trust calculation and device classification for dynamic access control. The proposed scheme is implemented, tested and deployed on Node MCU and same mechanism is also simulated on NS-2 for large number of nodes. This access control model support Scalability, Heterogeneity, Privacy, Trust, Selective disclosure, Principle of least privileges, and lightweight calculation features. Results of this models proves that it gives good performance as compared to existing scheme in terms of scalability, throughput and delay. As number of devices increase it does not degrade performance. This mechanism is also protected against the Man-in-the-Middle attack, Sniffing attack, Session Hijacking attacks and Injection attacks. It required less time to detect and resist those attacks.