bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae)
Authors: Dias Alyne Cunha Alves , Teixeira Aurea Vieira , Lima Bezerra Flaviana , Andriolo Aline , Silva Alexandre de A 
Published: November 2022

Abstract
Toxins and attractants have been studied and used in sugar baits for mosquitoes. However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality. Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae). Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates. Subsequently, different concentrations of IVM (0.312–100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values. Engorgement on the baits was observed under a stereomicroscope, and mortality was followed up to 48 h after feeding. In general, more mosquitoes engorged on TSB regardless of the sugar concentration, while higher concentrations of sugar in ATSBs resulted in higher numbers of mosquitoes engorging. A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations. No such effect was observed for males.
Foster Caregivers’ Perceptions of Children’s Sleep Patterns, Problems, and Environments
Authors: McGlinchey Eleanor L , Rigos Priscilla , Kim Josephine S , Muñoz Nogales Josefina , Valentine Madeline , Kim Jinu , Ripple Carol H , Wolfson Amy R , Alfano Candice A 
Published: November 2022

AbstractObjectiveThe disparity of problems, impairments, and disorders among children in foster care is well-documented and spans virtually every domain of functioning. Sleep, however, has received minimal attention among this vulnerable group, which is concerning given the multitude of ways sleep affects children’s development, health, and behavior.MethodsA total of 485 foster caregivers from across the United States completed a survey including quantitative items and qualitative, open-ended questions about sleep and related health and behavior for one child (M = 6.4 years, SD = 2.2; range 4–11 years) currently in their care.ResultsOverall, caregivers reported developmentally appropriate child sleep and wake times; however, difficulty falling asleep (avg. 46 min per night) and staying asleep (avg. 34 min awake overnight) were common. Additionally, a high prevalence of sleep-related problems was reported including moving to someone else’s bed during the night (85.8%), nightmares (51.2%), sleep terrors (26.4%), snoring (32.8%), bedwetting (31.6%), and teeth grinding (21.8%). Qualitative responses indicated emotional and behavioral challenges at bedtime, particularly elevated fear, and anxiety.ConclusionsFindings are consistent with previous work finding significant health disparities among children placed in foster care. Results highlight a need for trauma-informed, behavioral sleep interventions for this pediatric population which might serve to reduce other health disparities.
Acquisition of Enterobacterales carrying the colistin resistance genemcrfollowing travel to the tropics
Authors: Rondinaud Emilie , Clermont Olivier , Petitjean Marie , Ruppé Etienne , Esposito-Farèse Marina , Nazimoudine Anissa ,   The VOYAG-R Study Group, Coignard Bruno , Matheron Sophie , Andremont Antoine , Denamur Erick , Armand-Lefevre Laurence 
Published: November 2022

AbstractBackgroundColistin is an antibiotic of last resort in the management of highly drug-resistant Enterobacterales infections. Travel to some destinations presents a high risk of acquiring multidrug-resistant Enterobacterales, but little data are available on the risk of acquiring colistin-resistant strains. Here, we use the VOYAG-R sample collection (2012–2013) in order to evaluate the rate of acquisition of colistin-resistant Enterobacterales, excluding species with intrinsic resistance (CRE), following travel to tropical regions.MethodsA total of 574 frozen stool samples of travellers returning from tropical regions were screened for colistin-resistant strains using ChromID Colistin R agar (bioMerieux®) after pre-enrichment culture with 1 mg/L of colistin. Genomes were obtained by Illumina sequencing and genetic determinants of colistin resistance (mutational events and mcr genes) were searched.ResultsA total of 22 travellers (3.8%) acquired colistin-resistant Enterobacterales carrying an mcr gene. Acquisition rates varied between visited regions: 9.2% (18/195) for Asia (southeast Asia: 17/18), 2.2% (4/184) for Latin America (Peru: 4/4) and 0% from Africa (0/195). Acquired strains were predominantly Escherichia coli (92%) and carried mostly the mcr-1 variant (83%). Escherichia coli strains belonged mainly to commensal phylogroups A and B1, and were genetically highly diverse (5 non-clonal sequence type (ST)10 and 17 ST singletons). Only four non mcr colistin-resistant strains (two E. coli and two Enterobacter cloacae complex) were identified. Among all the strains, two also carried extended-spectrum beta-lactamase genes.ConclusionsTravel to tropical regions, and particularly to Southeast Asia, is a risk factor for the acquisition of mcr-carrying Enterobacterales. This study highlights the community dissemination of mcr in humans as early as 2012, 4 years prior to its first published description.
The galaxy size to halo spin relation of disc galaxies in cosmological hydrodynamical simulations
Authors: Yang Hang , Gao Liang , Frenk Carlos S , Grand Robert J J , Guo Qi , Liao Shihong , Shao Shi 
Published: November 2022

ABSTRACT
In the standard disc galaxy formation model, the sizes of galactic discs are tightly related to the spin parameters λ of their dark matter haloes. The model has been wildly adopted by various semi-analytical galaxy formation models which have been extremely successful to interpret a large body of observational data. However, the size–λ correlation was rarely seen in most modern hydrodynamical simulations of galaxy formation. In this short paper, we make use of 4 sets of large hydrodynamical simulations to explore the size–spin parameter relation with a large sample of simulated disc galaxies and compare it with a popular disc galaxy formation model of Mo et al. (1998). Intriguingly, galactic sizes correlate with spin parameters of their dark matter haloes in the simulations developed by the IllustrisTNG collaborations, albeit the relation does not always agree with prediction of MMW98 model overall stellar mass range we examined. There is also a size–spin correlation for the Milky Way analogies in the EAGLE simulations, while it is relatively weaker than that of the IllustrisTNG counterparts. For the dwarfs in the simulations from the EAGLE collaboration, there is NULL correlation. We conclude that either the detailed subgrid physics or hydrodynamics solvers account for the size-spin parameter relation, which will be explored in our future work.
Relating quasi-stationary one zone emission models to expanding relativistic shocks
Authors: Derishev Evgeny 
Published: November 2022

ABSTRACT
For an expanding spherical relativistic shock, we derive relations between the parameters of downstream emitting zone and the quantities measured by a distant observer. These relations are formulated in terms of dimensionless effective coefficients combined with self-evident dimensional estimates. Our calculations take into account evolution of the shock’s Lorentz factor, geometrical delay due to the shock’s front curvature, and angular dependence of Lorentz boost for frequency and brightness. The relations are designed primarily for application in gamma-ray burst afterglow studies, although they may have a broader use.
Using 44Ti emission to differentiate between thermonuclear supernova progenitors
Authors: Kosakowski Daniel , Ugalino Mark Ivan , Fisher Robert , Graur Or , Bobrick Alexey , Perets Hagai B 
Published: November 2022

ABSTRACT
The radioisotope 44Ti is produced through α-rich freezeout and explosive helium burning in type Ia supernovae (SNe Ia). In this paper, we discuss how the detection of 44Ti, either through late-time light curves of SNe Ia, or directly via gamma-rays, can uniquely constrain the origin of SNe Ia. In particular, building upon recent advances in the hydrodynamical simulation of helium-ignited double white dwarf binaries, we demonstrate that the detection of 44Ti in a nearby SN Ia or in a young Galactic supernova remnant (SNR) can discriminate between the double-detonation and double-degenerate channels of sub-Chandrasekhar (sub-MCh) and near-Chandrasekhar (near-MCh) SNe Ia. In addition, we predict that the late-time light curves of calcium-rich transients are entirely dominated by 44Ti.
Comparative analysis of low-field magnetic resonance imaging in patients with rheumatoid arthritis treated with certolizumab pegol or infliximab
Authors: Hagiwara Shinya , Tsuboi Hiroto , Kuroda Yuki , Sawabe Tomonori , Uematsu Nana , Kawashima Fumina , Sugita Toshiki , Terasaki Mayu , Honda Fumika , Yagishita Mizuki , Kondo Yuya , Sumida Takayuki , Matsumoto Isao 
Published: November 2022

ABSTRACT

Objectives
The aim is to clarify the differences in magnetic resonance imaging (MRI) findings between rheumatoid arthritis (RA) patients treated with certolizumab pegol (CZP) and infliximab (IFX).

Methods
The study included RA patients who received CZP or IFX and were examined with low-field MRI (compacTscan; compact magnetic resonance imaging) at the beginning and again within 6 months of treatment initiation. Comparisons were made regarding background, clinical course, and differences in MRI findings following initiation of tumour necrosis factor inhibitors between the CZP and IFX treatment groups. MRI findings were evaluated by scoring erosion, bone marrow oedema (BME), and synovitis.

Results
Ten cases in CZP and 18 cases in IFX group were compared. The biologic disease-modifying antirheumatic drug–naïve rate in the IFX group was significantly higher than that in the CZP group. After 6 months, disease activities were significantly decreased from baseline in both groups. Erosion score did not change significantly in both groups after 6 months. BME score was significantly decreased in the CZP group after 6 months, whereas in the IFX group, there was no significant change. Synovitis score was significantly decreased in both groups after 6 months.

Conclusions
The findings of our study suggest that, in patients with RA, CZP might improve BME more effectively than IFX.
Inductively coupled plasma mass spectrometry based urine metallome to construct clinical decision models for autism spectrum disorder
Authors: Liu Aiping , Cai Chunquan , Wang Zhangxing , Wang Bin , He Juntao , Xie Yanhong , Deng Honglian , Liu Shaozhi , Zeng Shujuan , Yin Zhaoqing , Wang Mingbang 
Published: November 2022

Abstract

Background
The global prevalence of autism spectrum disorder (ASD) is on the rise, and high levels of exposure to toxic heavy metals may be associated with this increase. Urine analysis is a noninvasive method for investigating the accumulation and excretion of heavy metals. The aim of this study was to identify ASD-associated urinary metal markers.

Methods
Overall, 70 children with ASD and 71 children with typical development (TD) were enrolled in this retrospective case–control study. In this metallomics investigation, inductively coupled plasma mass spectrometry was performed to obtain the urine profile of 27 metals.

Results
Children with ASD could be distinguished from children with TD based on the urine metal profile, with ASD children showing an increased urine metal Shannon diversity. A metallome-wide association analysis was used to identify seven ASD-related metals in urine, with cobalt, aluminum, selenium, and lithium significantly higher, and manganese, mercury, and titanium significantly lower in the urine of children with ASD than in children with TD. The least absolute shrinkage and selection operator (LASSO) machine learning method was used to rank the seven urine metals in terms of their effect on ASD. On the basis of these seven urine metals, we constructed a LASSO regression model for ASD classification and found an area under the receiver operating characteristic curve of 0.913. We also constructed a clinical prediction model for ASD based on the seven metals that were different in the urine of children with ASD and found that the model would be useful for the clinical prediction of ASD risk.

Conclusions
The study findings suggest that altered urine metal concentrations may be an important risk factor for ASD, and we recommend further exploration of the mechanisms and clinical treatment measures for such alterations.
EMPIAR: the Electron Microscopy Public Image Archive
Authors: Iudin Andrii , Korir Paul K , Somasundharam Sriram , Weyand Simone , Cattavitello Cesare , Fonseca Neli , Salih Osman , Kleywegt Gerard J , Patwardhan Ardan 
Published: November 2022

AbstractPublic archiving in structural biology is well established with the Protein Data Bank (PDB; wwPDB.org) catering for atomic models and the Electron Microscopy Data Bank (EMDB; emdb-empiar.org) for 3D reconstructions from cryo-EM experiments. Even before the recent rapid growth in cryo-EM, there was an expressed community need for a public archive of image data from cryo-EM experiments for validation, software development, testing and training. Concomitantly, the proliferation of 3D imaging techniques for cells, tissues and organisms using volume EM (vEM) and X-ray tomography (XT) led to calls from these communities to publicly archive such data as well. EMPIAR (empiar.org) was developed as a public archive for raw cryo-EM image data and for 3D reconstructions from vEM and XT experiments and now comprises over a thousand entries totalling over 2 petabytes of data. EMPIAR resources include a deposition system, entry pages, facilities to search, visualize and download datasets, and a REST API for programmatic access to entry metadata. The success of EMPIAR also poses significant challenges for the future in dealing with the very fast growth in the volume of data and in enhancing its reusability.
MirDIP 5.2: tissue context annotation and novel microRNA curation
Authors: Hauschild Anne-Christin , Pastrello Chiara , Ekaputeri Gitta Kirana Anindya , Bethune-Waddell Dylan , Abovsky Mark , Ahmed Zuhaib , Kotlyar Max , Lu Richard , Jurisica Igor 
Published: November 2022

AbstractMirDIP is a well-established database that aggregates microRNA-gene human interactions from multiple databases to increase coverage, reduce bias, and improve usability by providing an integrated score proportional to the probability of the interaction occurring. In version 5.2, we removed eight outdated resources, added a new resource (miRNATIP), and ran five prediction algorithms for miRBase and mirGeneDB. In total, mirDIP 5.2 includes 46 364 047 predictions for 27 936 genes and 2734 microRNAs, making it the first database to provide interactions using data from mirGeneDB. Moreover, we curated and integrated 32 497 novel microRNAs from 14 publications to accelerate the use of these novel data. In this release, we also extend the content and functionality of mirDIP by associating contexts with microRNAs, genes, and microRNA–gene interactions. We collected and processed microRNA and gene expression data from 20 resources and acquired information on 330 tissue and disease contexts for 2657 microRNAs, 27 576 genes and 123 651 910 gene–microRNA–tissue interactions. Finally, we improved the usability of mirDIP by enabling the user to search the database using precursor IDs, and we integrated miRAnno, a network-based tool for identifying pathways linked to specific microRNAs. We also provide a mirDIP API to facilitate access to its integrated predictions. Updated mirDIP is available at https://ophid.utoronto.ca/mirDIP.
TERRA regulates DNA G-quadruplex formation and ATRX recruitment to chromatin
Authors: Tsai Ru-Xuan , Fang Kuo-Chen , Yang Po-Cheng , Hsieh Yu-Hung , Chiang I-Tien , Chen Yunfei , Lee Hun-Goo , Lee Jeannie T , Chu Hsueh-Ping Catherine 
Published: November 2022

Abstract
The genome consists of non-B-DNA structures such as G-quadruplexes (G4) that are involved in the regulation of genome stability and transcription. Telomeric-repeat containing RNA (TERRA) is capable of folding into G-quadruplex and interacting with chromatin remodeler ATRX. Here we show that TERRA modulates ATRX occupancy on repetitive sequences and over genes, and maintains DNA G-quadruplex structures at TERRA target and non-target sites in mouse embryonic stem cells. TERRA prevents ATRX from binding to subtelomeric regions and represses H3K9me3 formation. G4 ChIP-seq reveals that G4 abundance decreases at accessible chromatin regions, particularly at transcription start sites (TSS) after TERRA depletion; such G4 reduction at TSS is associated with elevated ATRX occupancy and differentially expressed genes. Loss of ATRX alleviates the effect of gene repression caused by TERRA depletion. Immunostaining analyses demonstrate that knockdown of TERRA diminishes DNA G4 signals, whereas silencing ATRX elevates G4 formation. Our results uncover an epigenetic regulation by TERRA that sequesters ATRX and preserves DNA G4 structures.
A Scoping Review and Meta-analysis of the Use of Remote Biochemical Verification Methods of Smoking Status in Tobacco Research
Authors: Thrul Johannes , Howe Carol L , Devkota Janardan , Alexander Adam , Allen Alicia M , Businelle Michael S , Hébert Emily T , Heffner Jaimee L , Kendzor Darla E , Ra Chaelin K , Gordon Judith S 
Published: November 2022

AbstractIntroductionIncreasing digital delivery of smoking cessation interventions has resulted in the need to employ novel strategies for remote biochemical verification.Aims and MethodsThis scoping review and meta-analysis aimed to investigate best practices for remote biochemical verification of smoking status. The scientific literature was searched for studies that reported remotely obtained (not in-person) biochemical confirmation of smoking status (ie, combustible tobacco). A meta-analysis of proportions was conducted to investigate key outcomes, which included rates of returned biological samples and the ratio of biochemically verified to self-reported abstinence rates.ResultsA total of 82 studies were included. The most common samples were expired air (46%) and saliva (40% of studies), the most common biomarkers were carbon monoxide (48%) and cotinine (44%), and the most common verification methods were video confirmation (37%) and mail-in samples for lab analysis (26%). Mean sample return rates determined by random-effects meta-analysis were 70% for smoking cessation intervention studies without contingency management (CM), 77% for CM studies, and 65% for other studies (eg, feasibility and secondary analyses). Among smoking cessation intervention studies without CM, self-reported abstinence rates were 21%, biochemically verified abstinence rates were 10%, and 47% of individuals who self-reported abstinence were also biochemically confirmed as abstinent.ConclusionsThis scoping review suggests that improvements in sample return rates in remote biochemical verification studies of smoking status are needed. Recommendations for reporting standards are provided that may enhance confidence in the validity of reported abstinence rates in remote studies.ImplicationsThis scoping review and meta-analysis included studies using remote biochemical verification to determine smoking status. Challenges exist regarding implementation and ensuring high sample return rates. Higher self-reported compared to biochemically verified abstinence rates suggest the possibility that participants in remote studies may be misreporting abstinence or not returning samples for other reasons (eg, participant burden, inconvenience). Remote biochemical confirmation of self-reported smoking abstinence should be included in smoking cessation studies whenever feasible. However, findings should be considered in the context of challenges to sample return rates. Better reporting guidelines for future studies in this area are needed.
Single-Cell RNA Sequencing of Arabidopsis Leaf Tissues Identifies Multiple Specialized Cell Types: Idioblast Myrosin Cells and Potential Glucosinolate-Producing Cells
Authors: Maeda Taro , Sugano Shigeo S , Shirakawa Makoto , Sagara Mayu , Ito Toshiro , Kondo Satoshi , Nagano Atsushi J 
Published: November 2022

Abstract
The glucosinolate–myrosinase defense system (GMDS), characteristic of Brassicales, is involved in plant defense. Previous single-cell transcriptomic analyses have reported the expression profiles of multiple GMDS-related cell types (i.e. myrosinase-rich myrosin idioblasts and multiple types of potential glucosinolate synthetic cells as well as a candidate S-cell for glucosinolate accumulation). However, differences in plant stages and cell-type annotation methods have hindered comparisons among studies. Here, we used the single-cell transcriptome profiles of extended Arabidopsis leaves and verified the distribution of previously used markers to refine the expression profiles of GMDS-associated cell types. Moreover, we performed beta-glucuronidase promoter assays to confirm the histological expression patterns of newly obtained markers for GMDS-associated candidates. As a result, we found a set of new specific reporters for myrosin cells and potential glucosinolate-producing cells.
The small peptide CEP1 and the NIN-like protein NLP1 regulate NRT2.1 to mediate root nodule formation across nitrate concentrations
Authors: Luo Zhenpeng , Wang Jiang , Li Fuyu , Lu Yuting , Fang Zijun , Fu Mengdi , Mysore Kirankumar S , Wen Jiangqi , Gong Jiming , Murray Jeremy D , Xie Fang 
Published: November 2022

Abstract
Legumes acquire fixed nitrogen (N) from the soil and through endosymbiotic association with diazotrophic bacteria. However, establishing and maintaining N2-fixing nodules are expensive for the host plant, relative to taking up N from the soil. Therefore, plants suppress symbiosis when N is plentiful and enhance symbiosis when N is sparse. Here, we show that the nitrate transporter MtNRT2.1 is required for optimal nodule establishment in Medicago truncatula under low-nitrate conditions and the repression of nodulation under high-nitrate conditions. The NIN-like protein (NLP) MtNLP1 is required for MtNRT2.1 expression and regulation of nitrate uptake/transport under low- and high-nitrate conditions. Under low nitrate, the gene encoding the C-terminally encoded peptide (CEP) MtCEP1 was more highly expressed, and the exogenous application of MtCEP1 systemically promoted MtNRT2.1 expression in a compact root architecture 2 (MtCRA2)-dependent manner. The enhancement of nodulation by MtCEP1 and nitrate uptake were both impaired in the Mtnrt2.1 mutant under low nitrate. Our study demonstrates that nitrate uptake by MtNRT2.1 differentially affects nodulation at low- and high-nitrate conditions through the actions of MtCEP1 and MtNLP1.