bluuebunny/crossref_metadata_2025_split huggingface dataset:
A newly developed and validated LC–MS/MS method for measuring 7-dehydrocholesterol (7DHC) concentration in human skin: a tool for vitamin D photobiology research
Authors: Borecka Oktawia , Rhodes Lesley E. , Webb Ann R. , Dutton John J. , Fraser William D. 
Published: July 2022

Abstract
Background
UVB absorption by 7-dehydrocholesterol (7DHC) in the skin triggers the production of vitamin D and its metabolites, which maintain calcium homeostasis. Detection and measurement of 7DHC in skin using modern liquid chromatography–tandem mass spectrometry (LC–MS/MS) techniques have been lacking, yet there is need for such a technique to provide more information on 7DHC concentration and its UVB responses in human skin.

Objectives
To develop and validate a reliable method to measure 7DHC concentration in skin.

Methods
Human skin punch biopsies of 5 mm diameter obtained through the Manchester Skin Health Biobank were utilised. 7DHC was extracted with ethyl acetate:methanol 1:1 (v/v) and derivatised using 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), to allow for improved ionisation of 7DHC through Electrospray Ionisation Mass Spectrometry (ESI–MS). Solid supported liquid extraction (SLE) was also employed to allow the removal of larger lipids from 7DHC and minimise potential matrix effects.

Results
The LC–MS/MS assay satisfied International Council for Harmonisation research standards for method validation. Calibration curve was linear with a typical r2 of 0.997, coefficient of variation was 11.1% and 4.32% for inter-assay and intra-assay imprecision, respectively. Lower limit of quantification was 1.6 µg/g and upper limit of quantification was 100 µg/g, SLE recovery of 7DHC was on average 91.4%.

Conclusions
We have developed a robust, precise and accurate assay for the detection and quantification of 7DHC in small samples of human skin (0.2 cm2 surface area). This novel method of extraction and quantification will be valuable to future vitamin D photobiology research.

Graphical Abstract
A deep learning-based approach for electrical equipment remaining useful life prediction
Authors: Fu Huibin , Liu Ying 
Published: July 2022

AbstractElectrical equipment maintenance is of vital importance to management companies. Efficient maintenance can significantly reduce business costs and avoid safety accidents caused by catastrophic equipment failures. In the current context, predictive maintenance (PdM) is becoming increasingly popular based on machine learning approaches, while its research on electrical equipment such as low-voltage contactors is in its infancy. The failure modes are mainly fusion welding and explosion, and a few are unable to switch on. In this study, a data-driven approach is proposed to predict the remaining useful life (RUL) of the low-voltage contactor. Firstly, the three-phase alternating voltage and current records the life of electrical equipment by tracking the number of times it has been operated. Secondly, the failure-relevant features are extracted by using the time domain, frequency domain, and wavelet methods. Then, a CNN-LSTM network is designed and used to train an electrical equipment RUL prediction model based on the extracted features. An experimental study based on ten datasets collected from low-voltage AC contactors reveals that the proposed method shows merits in comparison with the prevailing deep learning algorithms in terms of MAE and RMSE.
The Dartmouth Atlas of Health Care – bringing health care analyses to health systems, policymakers, and the public
Authors: Bronner Kristen K. , Goodman David C. 
Published: July 2022

AbstractIn 1996, the Dartmouth Atlas of Health Care pioneered the dissemination of policy-relevant population-based measurement and analysis that revealed both weaknesses and opportunities in the United States health care system by focusing on regional and hospital variation in utilization, quality, and costs. Built on a growing foundation of peer-reviewed research, the Atlas produced more than 40 reports over the next 25 years addressing a wide range of pressing health care problems. The project’s publications and website also provided regional and hospital-specific data to health systems, governmental jurisdictions, health care stakeholders, and the public. The Atlas’ methods and its conceptual framework have been widely disseminated in North America and the United Kingdom, and, more recently, in Europe, South America, Asia, and Oceania. This paper discusses the origins of the Atlas from Dr. John Wennberg’s early studies, the scaling up of data, methods, and policy-relevant findings, and its incorporation into the more general fields of health services research, policy development, and clinical improvement.
Burden of care among primary caregivers’ of spinal cord injury patients attending a tertiary care center in Eastern Nepal
Authors: Sinha Pramila , Mehta Ram Sharan , Parajuli Pushpa , Chaudhary Pashupati , Kushwaha Ramayan Prasad 
Published: July 2022

Abstract
Background
Nepal is a resource-constrained country where primary caregivers of spinal cord injury (SCI) patients face increasing physical, social, emotional, and economic burdens. This study aimed to assess the level of primary caregiver burden and the association between caregiver burden and sociodemographic factors of SCI patients and their caregivers.

Methods
The burden of caregivers was evaluated in 71 primary caregivers through face-to-face interviews using the Zarit Burden Interview Scale (ZBI-22). Based on the total ZBI-22 score, caregiver burden was classified as little or no, mild to moderate, moderate to severe, and severe.

Results
The mean caregiver burden score was 46.28 ± 12.05. Moderate to severe burden was more common (63.4%), followed by severe (18.3%), mild to moderate (11.3%), and little or no burden (7.0%). Caregivers were more worried about “what the future holds for patients (3.07 ± 1.22) and what should do more for patients (3.04 ± 1.01)”. Several sociodemographic factors, such as marital status (P = 0.009), activities of daily living (P = 0.038), monthly family income (P = 0.023), and time spent caring for patients (P = 0.028), were statistically significantly associated with the burden on primary caregivers.

Conclusions
Our results showed that primary caregivers experienced a high burden of care, primarily related to fear and uncertainty about the patient’s future, patient’s dependency, caregiver exhaustion and finances. Several sociodemographic factors of patients and their caregivers were associated with the burden of the primary caregivers.
Measurement of Disaster Literacy in Turkish Society: Disaster Literacy Scale (DLS) Design and Development Process
Authors: Çalışkan Cüneyt , Üner Sarp 
Published: July 2022

Abstract

Objective:
This study aimed to design and develop a self-report Disaster Literacy Scale (DLS) tool that could evaluate the knowledge and skills of an individual specific to Turkish society.

Method:
Item development, expert opinions, language control, pilot study and field testing processes were monitored in the measurement tool based on a conceptual model and recognition.

Results:
23 items were taken out since their common variance values (>0,508, >0.500, >0.500, >0.400, respectively) and factor load relationship (>0.46, >0.50, >0.50, >0.50 and >0.55, respectively) in the mitigation, preparedness, response and recovery phases of the Exploratory Factor Analysis were insufficient. The Cronbach Alpha value of the remaining 61 items in the Disaster Literary Scale is 0.954 and between 0.83-0.88 in lower dimensions. DLS scoring was standardized between 0-50 points.

Conclusions:
The objectives, scopes, limitations and steps of the design and development process of the Disaster Literacy Scale were given in detail and made understandable for other societies. The Disaster Literacy Scale was developed as a self-report scale that could evaluate the knowledge and skills of Turkish society in disasters. The Disaster Literacy Scale is, therefore, expected to be accepted in more countries to improve the understanding of disaster literacy in different societies.
The PrEP Laboratory Service Gap: Applying Implementation Science Strategies to Bring PrEP Coverage to Scale in the United States
Authors: Siegler Aaron , Sullivan Patrick 
Published: July 2022

AbstractUsing an implementation science framework, we detail how a national system for covering both standard and telemedicine laboratory testing would support a national PrEP program. Implementation strategies that will facilitate success include minimizing provider burden through uncomplicated billing systems and minimizing patient burden through centralized, online access systems. We anticipate that providing telemedicine and in-person laboratory testing options will optimize PrEP care by making it less burdensome, leading to cost-effective healthcare and improved population health.
“But All Had Great Reason to Dread!”: Religion, Affect, and Conspiracy in the Stamp Act Crisis
Authors: Urich Joshua D. 
Published: July 2022

AbstractThis article explores affective and emotional components of conspiracism in the 1765 Boston Stamp Act Crisis. Once a common subject in the study of Revolutionary America, conspiracism has disappeared from the historiography in recent decades. I argue that this is a serious oversight in understanding religion in the Revolutionary era. Unmasking conspiratorial plots against colonial liberties was a religious experience in the colonies, simultaneously imbuing liberty with a felt sense of sacredness and forging an emotional separation between the colonies and England. In making this claim, this article aims to demonstrate how scholars of religion might incorporate affect into the historical study of religion. Attention to affective cues, particularly in religious texts, sheds light on the phenomenology of historical religion. Through such analysis, we can begin to understand how religious communities formed through affective connections to the sacred, what that sacred felt like, and how bodily and emotional experience shaped reactions to violations of the sacred. In the case of the Stamp Act Crisis, conspiracism, anti-Catholicism, and satanic symbolism sparked an affectively charged moment characterized by fear, disgust, and anger. As colonists participated in unmasking supposed Catholic and Parliamentary conspiracies against them, they created a community united around scared liberty.
Conifer tree species and age as drivers of epiphytic lichen communities in northern European production forests
Authors: Petersson Lisa , Lariviere Delphine , Holmström Emma , Fritz Örjan , Felton Adam 
Published: July 2022

AbstractThe epiphytic lichen species richness and community composition was compared for 600 living trees distributed within the interior of 60 Scots pine and Norway spruce monoculture stands in southern Sweden. A higher species richness, and more unique species, was found on trees of Scots pine than of Norway spruce, and distinctive communities were associated with the two tree species. Lichen species composition also shifted between the 30-, 55- and 80-year-old stands, although there was no significant difference in species richness between the different age classes. Tree species and age of the stand explained most of the variation in community composition (41%), with additional variance explained by lichen proximity to the ground (6%) and aspect (1%) (northern/southern side of trunk). Scots pine and Norway spruce share many attributes, such as both being conifers with acidic bark properties and having a similar geographical distribution in Fennoscandia. However, our study showed that species richness and community composition can nevertheless diverge in stands dominated by these two tree species. Since the occurrence of red-listed species was low in these stands, we suggest that 80-year rotations are not long enough for the occurrence of the many rare and specialized lichen species that require old forest structures and long forest continuity in this region.
Friendships, loneliness and psychological wellbeing in older adults: a limit to the benefit of the number of friends
Authors: Thompson Alexandra , Smith Michael A. , McNeill Andrew , Pollet Thomas V. 
Published: July 2022

AbstractLoneliness is linked to many negative health outcomes and places strain on the economy and the National Health Service in the United Kingdom. To combat these issues, the determinants of loneliness need to be fully understood. Although friendships have been shown to be particularly important in relation to loneliness in older adults, this association has thus far not been explored more closely. Our exploratory study examines the relationship between number of friends and loneliness, depression, anxiety and stress in older adults. Data were obtained from 335 older adults via completion of an online survey. Measures included loneliness (UCLA Loneliness Scale version 3), depression, anxiety and stress (Depression Anxiety Stress Scales DASS-21). Participants also reported their number of close friends. Regression analyses revealed an inverse curvilinear relationship between number of friends and each of the measures tested. Breakpoint analyses demonstrated a threshold for the effect of number of friends on each of the measures (loneliness = 4, depression = 2, anxiety = 3, stress = 2). The results suggest that there is a limit to the benefit of increasing the number of friends in older adults for each of these measures. The elucidation of these optimal thresholds can inform the practice of those involved in loneliness interventions for older adults. These interventions can become more targeted; focusing on either establishing four close friendships, increasing the emotional closeness of existing friendships or concentrating resources on other determinants of loneliness in this population.
Epidemiological characteristics of extrapulmonary tuberculosis patients with or without pulmonary tuberculosis
Authors: Fang Yong , Zhou Qinqi , Li Lin , Zhou Yiming , Sha Wei 
Published: July 2022

Abstract
Our study aimed to investigate the epidemiology of extrapulmonary tuberculosis (EPTB) and analyse the epidemiological characteristics of EPTB patients with or without pulmonary tuberculosis (PTB). EPTB cases admitted in our hospital from January 2015 to December 2020 were included. Uni- and multi-variable logistic regression analysis was carried out to identify risk factors and prognostic factors of concomitant EPTB and PTB or exclusively EPTB. A total of 3488 EPTB patients were reviewed, including 2086 patients with concurrent PTB and EPTB, and 1402 patients with exclusively EPTB. Logistic regression analysis showed that age >60 years (OR = 1.674, 95% CI = 1.438–1.949, P < 0.001) and female (OR = 1.325, 95% CI = 1.155–1.520, P < 0.001) were risk factors of exclusively EPTB, while co-morbidities (OR = 0.676, 95% CI = 0.492–0.929, P = 0.016) and severe symptoms (OR = 0.613, 95% CI = 0.405–0.929, P = 0.021) were risk factors for concurrence of EPTB and PTB. Age >60 years was an independent prognostic factor in EPTB patients with or without PTB (HR = 11.059, 95%CI = 5.097–23.999, P < 0.001; HR = 23.994, 95%CI = 3.093–186.151, P = 0.0020). Female gender was an independent prognostic factor in patients with concurrent PTB and EPTB (HR = 23.994, 95%CI = 3.093–186.151, P = 0.002). Our study disclosed the differential epidemiological characteristics of EPTB patients with or without PTB in China.
Ethical Solutions to the Problem of Organ Shortage
Authors: Sterri Aksel Braanen , Regmi Sadie , Harris John 
Published: July 2022

AbstractOrgan shortage is a major survival issue for millions of people worldwide. Globally 1.2 million people die each year from kidney failure. In this paper, we critically examine and find lacking extant proposals for increasing organ supply, such as opting in and opt out for deceased donor organs, and parochial altruism and paired kidney exchange for live organs. We defend two ethical solutions to the problem of organ shortage. One is to make deceased donor organs automatically available for transplant without requiring consent from the donor or their relatives. The other is for society to buy nonvital organs in a strictly regulated market and provide them to people in need for free.
Cases Abusing Brain Death Definition in Organ Procurement in China
Authors: Paul Norbert W. , Allison Kirk C. , Li Huige 
Published: July 2022

AbstractOrgan donation after brain death has been practiced in China since 2003 in the absence of brain death legislation. Similar to international standards, China’s brain death diagnostic criteria include coma, absence of brainstem reflexes, and the lack of spontaneous respiration. The Chinese criteria require that the lack of spontaneous respiration must be verified with an apnea test by disconnecting the ventilator for 8 min to provoke spontaneous respiration. However, we have found publications in Chinese medical journals, in which the donors were declared to be brain dead, yet without an apnea test. The organ procurement procedures started with initiating “intratracheal intubation for mechanical ventilation after brain death,” indicating that a brain death diagnosis was not performed. The purpose of the intubation was not to resuscitate the patient but rather was directly related to facilitating the explantation of organs. Moreover, it was unmistakably stated in two of these publications that the cardiac arrest was induced in these patients without brain death determination by cold St. Thomas cardioplegic solution or other cold myocardial protection solutions. This means that the condition of these donors neither met the criteria of brain death nor that of cardiac death. In other words, the “donor organs” may well have been procured in these cases from living human beings. Thus, brain death definition is abused in China by some individuals for organ harvesting, and a systematic investigation is needed to clarify the situation of organ donation after brain death in China.
Identification of field resistance to HPPD-inhibiting herbicides in wild radish (Raphanus raphanistrum)
Authors: Busi Roberto , Zhang Bowen , Goggin Danica , Bryant Glenn , Beckie Hugh J. 
Published: July 2022

AbstractThe control of multiple-resistant wild radish (Raphanus raphanistrum L.) populations in no-till Australian wheat (Triticum aestivum L.) crops has relied upon 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides over the last decade. Two R. raphanistrum populations identified as putatively resistant to pyrasulfotole + bromoxynil in an initial large-scale screening trial were characterized and confirmed to be 5- to 8-fold (comparison of LD50 values) less sensitive than the susceptible control population to the HPPD inhibitor pyrasulfotole when plants were treated at the 4-leaf stage. The two pyrasulfotole-resistant populations exhibited up to 4-fold resistance to the coformulated herbicide mixture pyrasulfotole + bromoxynil and up to 9- and 11-fold cross-resistance to mesotrione and topramezone postemergence, respectively. A small-plot trial was conducted in the field from which of one of the populations suspected of resistance was originally collected. Pyrasulfotole + bromoxynil or topramezone + bromoxynil applied postemergence delivered reduced R. raphanistrum control (79% to 87%), whereas mesotrione applied preemergence was >99% effective. We report here the first case of field resistance to HPPD-inhibiting herbicides in R. raphanistrum, caused by 12 yr of continuous reliance on that mode of action. The mitigation of herbicide resistance in continuous no-till cropping requires a constant optimization of the herbicide technology via alternation and mixtures of multiple sites of action, use of preemergence herbicides, and ensuring postemergence herbicides are applied at the most sensitive plant growth stages.
Small fragments of hyaluronan are increased in individuals with obesity and contribute to low-grade inflammation through TLR-mediated activation of innate immune cells
Authors: Romo Mònica , López-Vicario Cristina , Pérez-Romero Noelia , Casulleras Mireia , Martínez-Puchol Ana Isabel , Sánchez Belén , Flores-Costa Roger , Alcaraz-Quiles José , Duran-Güell Marta , Ibarzábal Ainitze , Espert Juan José , Clària Joan , Titos Esther 
Published: July 2022

Abstract
Background and aim
Extracellular matrix (ECM) components released during excessive fat mass expansion are considered potential endogenous danger/alarm signals contributing to innate immune system activation. The aim of the current study was to specifically measure plasma levels of low molecular weight (LMW) hyaluronan (HA) and to evaluate its role as pro-inflammatory damage-associated molecular pattern (DAMP) on leukocyte response in the context of human obesity.

Subjects and methods
Participants were selected according to their body mass index (BMI, kg/m2) as non-obese (BMI < 29.9, n = 18) and obese (BMI > 29.9, n = 33). Plasma samples were size-dependent fractionated using ion-exchange chromatography to specifically obtain LMW HA fractions that were subsequently quantified by ELISA. Cell incubation experiments with synthetic HA molecules were performed on freshly Ficoll-isolated neutrophils (PMN) and peripheral blood monocytes (PBMC). Leukocyte and adipose tissue gene expression was assessed by real-time PCR and NF-κB activation by western blot. Plasma cytokine levels were measured by fluorescent bead-based (Luminex) immunoassay.

Results
We observed a statistically significant increase in the circulating levels of HA fragments of LMW in individuals with obesity which were consistent with significant up-regulated expression of the LMW HA synthesizing enzyme hyaluronan synthase-1 (HAS-1) in obese adipose tissue. Gene expression assessment of HA receptors revealed up-regulated levels for TLR2 in both obese PMN and PBMC. Synthetic HA molecules of different sizes were tested on leukocytes from healthy donors. LMW HA fragments (15–40 kDa) and not those from intermediate molecular sizes (75–350 kDa) induced a significant up-regulation of the expression of major pro-inflammatory cytokines such as IL-1β, MCP-1 and IL-8 in PBMC. Importantly, LMW HA was able to induce the phosphorylation of IKK α/β complex supporting its pro-inflammatory role through NF-κB activation.

Conclusion
Circulating LMW HA molecules are elevated in obesity and may play an important role in triggering low-grade inflammation and the development of metabolic complications.
A high mobility air-stable n-type organic small molecule semiconductor with high UV–visible-to-NIR photoresponse
Authors: Guan Ying-Shi , Qiao Jing , Liang Yingying , Bisoyi Hari Krishna , Wang Chao , Xu Wei , Zhu Daoben , Li Quan 
Published: July 2022

AbstractAn organic semiconductor with high carrier mobility and efficient light absorption over a wide spectral range is of the most important yet challenging material for constructing a broadband responsive organic photodetector. However, the development of such organic semiconductors, especially for air-stable n-type organic small molecule semiconductors, is still at an early stage. Here we report the fabrication of high-performance n-type semiconducting crystalline nanosheets and the development of air-stable field-effect transistors, phototransistors, with high response over a broad spectrum. The n-type small molecule semiconductor is assembled into a crystalline nanosheet based on the solvent-phase interfacial self-assembly method. N-type field-effect transistors with high electron mobility are fabricated and their electrical performances exhibit excellent air stability. Impressively, the demonstrated phototransistors exhibit an ultrahigh responsivity over a wide spectral range from 365 to 940 nm, with a maximum photoresponsivity of 9.2 × 105 A W−1 and specific detectivity of 5.26 × 1013 Jones, which is the best performance among the reported n-type organic small molecule-based phototransistors.