bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transfer of brotizolam, periciazine, and sulpiride in cord blood and breast milk, and alprazolam in breast milk: a case report
Authors: Saito Jumpei , Tachibana Yoshiyuki , Wada Yuka Sano , Yakuwa Naho , Kawasaki Hiroyo , Suzuki Tomo , Sago Haruhiko , Yamatani Akimasa , Murashima Atsuko 
Published: April 2022

Abstract
Background
A high prevalence of mental disorders including depression, anxiety, somatoform, and dissociative disorder is reported during pregnancy, however, information on the transfer of antipsychotics across the placenta and into breast milk is limited. We evaluated brotizolam, periciazine and sulpiride in cord blood, maternal serum, and breast milk, and alprazolam in breast milk.

Case presentation
A 38-year-old woman with dissociative disorder was treated with brotizolam, propericiazine, and sulpiride during pregnancy and lactation, and alprazolam during lactation. The drug concentration ratios for both cord blood and maternal serum were 33.3 and 61.5% for brotizolam and sulpiride, respectively, and periciazine (metabolite of propericiazine) was not detected in the cord blood. In breast milk, alprazolam (0.9 ng/mL), sulpiride (445.8 ng/mL), and periciazine (0.3 ng/mL) concentrations were noted at 7.5 h after the last dose on postpartum, whereas brotizolam was not detected. The relative infant doses via breast milk were 1.4, 2.7 and 0.02% of the maternal daily dose, respectively. The neonate had no congenital anomalies and did not experience any severe withdrawal symptoms after birth.

Conclusion
Use of brotizolam, propericiazine, and sulpiride during pregnancy and lactation, and use of alprazolam during lactation were acceptable in this case.
Endurance rivalry and female choice jointly influence male mating success in the emerald treefrog (Zhangixalus prasinatus), a lek-chorusing anuran
Authors: Cheng Yuan-Cheng , Chen Yi-Huey , Chang Chunwen , Chuang Ming-Feng , Hsu Yuying 
Published: April 2022

Abstract
Background
Endurance rivalry and female choice are two important mechanisms of sexual selection in lek-breeding species. Endurance rivalry is when males compete for opportunities to mate by spending more time in leks than others (interaction-independent male-male competition). Because high-quality males can afford to have high lek attendance, females have a higher chance of mating with good-quality males even when they mate randomly. The good gene hypothesis proposes that females can pass good genes on to their offspring by choosing males that display elaborate morphological and/or behavioral traits that reflect the males’ genetic quality. The relative importance of lek attendance and female choice to males’ mating success in anurans is rarely evaluated. In this study, we investigated how these two mechanisms might jointly shape males’ morphological traits in the lek-chorusing emerald treefrog Zhangixalus prasinatus.

Results
Our results show that (1) male lek attendance is positively correlated with body size and condition, and males with higher lek attendance have higher mating success, (2) the dominant frequency of males’ advertisement calls are negatively correlated with body size and males producing lower frequency calls have higher mating success, (3) male body size, but not body condition, has a non-significant positive relationship with mating success and (4) females show preference for calls with lower dominant frequencies in two-choice playback.

Conclusions
Overall, both endurance rivalry and female choice play an important role in the mating success of male emerald treefrogs in the field and both are influenced by male body size/condition. By mating with males that have higher lek attendance and produce lower frequency calls, selection may indirectly favor larger males.
Dietary factors associated with gastric cancer - a review
Authors: Richa  , Sharma Neha , Sageena Geetanjali 
Published: April 2022

Abstract
Background
Cancer is considered one of the primary illnesses that cause morbidity and mortality in millions of people worldwide and due to its prevalence, there is undoubtedly an unmet need to discover novel anticancer drugs. As most of the target-specific anticancer drugs failed to achieve the expected result so far, new multi-targeted therapies using natural products have become significant. Natural products are readily applicable, inexpensive, accessible and acceptable therapeutic approaches with minimum cytotoxicity. This review explores the relationship between dietary factors and the probability of development of gastric cancer. Over the years, it has been proven that there are many natural products that have chemo-preventative effects such as vitamins, probiotics and prebiotics, green tea, and resveratrol on gastric cancer. Many investigations have looked into the links between dietary components and the risk of stomach cancer and a variety of protective and deleterious factors have been identified in our diet that is associated with gastric cancer. In this study, we have summarized the preventive and injurious components that affect the incidence of gastric cancer.

Conclusion
Based on the potential of natural products for the discovery of new compounds for the treatment of diseases, the purpose of the present review was to investigate the importance of dietary products mitigating the impacts due to the development of gastric cancer. The current analysis proves the protective as well as harmful roles of many elements which are found in our diet in the development of gastric cancer. Our findings can have significant public health implications in terms of gastric cancer prevention. There is an inherent need to offer an insight into benefits and risks, clinical trial designs future translational and cohort studies before effectively clinically translated.
A case of refractory systemic lupus erythematosus with monocytosis exhibiting somatic KRAS mutation
Authors: Law Sze-Ming , Akizuki Shuji , Morinobu Akio , Ohmura Koichiro 
Published: April 2022

Abstract
Background
Systemic lupus erythematosus (SLE), an autoimmune disorder that damages various organ systems, is caused by a combination of genetic and environmental factors. Although germline mutations of several genes are known to cause juvenile SLE, most of the susceptibility genetic variants of adult SLE are common variants of the population, somatic mutations that cause or exacerbate SLE have not been reported. We hereby report a refractory SLE case with monocytosis accompanying somatic KRAS mutation that have been shown to cause lupus-like symptoms.

Case presentation
A 60-year-old female patient who had been diagnosed with SLE was admitted to our hospital. Although prednisolone and tacrolimus treatments had kept her thrombocytopenia and anti-DNA Ab level at bay for more than 4 years, a diagnosis of transverse myelitis was made when she became acutely ill with pleocytosis. Elevated cells (predominately monocytes), protein, IgG, and IL-6 levels were also found in the cerebrospinal fluid (CSF) of the patient. Standard pulse treatments of methylprednisolone, high-dose of prednisolone, and intravenous cyclophosphamide in combination with plasma exchange could not alleviate the refractory neural and autoimmune manifestation. Monocytosis of peripheral blood was also noted. Flow cytometric analysis revealed elevated ratio of CD14+CD16+ atypical monocytes, which excluded the possibility of chronic myelomonocytic leukemia. Lupus-like symptoms with monocytosis reminded us of Ras-associated autoimmune leukoproliferative disorder, and Sanger sequencing of KRAS and NRAS genes from the patients’ peripheral blood mononuclear cells (PBMC), sorted CD3+ lymphocytes and CD14+ monocytes, and cerebrospinal fluid were performed. An activating KRAS somatic mutation was found in the patients’ DNA at the time of encephalomyelitis diagnosis.

Conclusion
Somatic mutations of some genes including KRAS may cause the refractoriness of SLE.
A three-factor benefits framework for understanding consumer preference for scented household products: psychological interactions and implications for future development
Authors: Herz Rachel S. , Larsson Maria , Trujillo Rafael , Casola Marisa C. , Ahmed Farah K. , Lipe Stacy , Brashear Morgan E. 
Published: April 2022

AbstractHumans have deliberately scented their environment for purpose or pleasure for millennia. In the contemporary marketplace most consumers prefer and purchase scented versions of common household products. However, the drivers of this consumer preference have not been elucidated. To explain the attraction to scent in household products we propose a novel three-factor framework, comprising functional benefits (malodor mitigation, base odor coverage, freshening), in-use experience benefits (cleanliness, efficacy, pleasure), and emotional benefits (increasing in confidence, mood and nostalgia). To support this framework, we present new data from a market research survey on US consumer purchasing habits and attitudes towards home cleaning, laundry, and air freshening products. Further substantiating our framework, a focused review of olfactory psychological science illustrating the central role of scent in cognition, wellbeing, motivated behavior, and social behavior, as well as sensory marketing research highlights the benefits and implications of scent in consumer household products. Based on our three-factor framework we go on to discuss the potential for scent to influence health and raise issues to consider (such as potential negative responding to fragranced products). We conclude by showcasing new opportunities for future research in olfactory science and on scented household products that can advance the positive impacts of scent.
Micro-credentials in leveraging emergency remote teaching: the relationship between novice users’ insights and identity in Malaysia
Authors: Kumar Jeya Amantha , Richard Rachel Jasmine , Osman Sharifah , Lowrence Kevin 
Published: April 2022

AbstractMicro-credentials have gained much popularity in recent years, and their popularity has skyrocketed due to emergency remote teaching instigated by the pandemic. It has been defined as a platform that provides credentials based on validated competencies. Nevertheless, in Malaysian HEI, such a concept is still novel and identifying insights on the benefits, challenges, and application are still scarce. Similarly, it was observed that there is a lack of observation on how students’ digital learning identity and their perception of professional relevance are influenced by such platform. Henceforth, based on the adapted enriched virtual model approach, a micro-credentials course was implemented to complement the new “normal” classes for a pre-service teacher’s instructional design course. A mixed-method triangulation design was used to explore the qualitative findings operationalized by open-ended questions (N = 74) with data obtained from the Digital Learning Identity Survey (DLIS) and Constructivist On-Line Learning Environment Survey (COLLES) (N = 72). The findings indicated that respondents had an overall positive perception of the use of micro-credentials to complement and overcome online learning challenges mainly due to substandard internet connectivity; nevertheless, they are unaware of the value of such credentials in their future profession. Conversely, their new identity as digital learners and experiences with a blended approach of online learning, especially with micro-credentials, was successful in shaping their identity as aspiring educators that embrace technology for teaching and learning.
A systematic review and recommendations for prom instruments for older people with frailty in emergency care
Authors: van Oppen James David , Alshibani Abdullah , Coats Timothy John , Graham Blair , Holch Patricia , Lalseta Jagruti , Mackintosh Nicola , Richardson Vivien , Riley Peter , Valderas Jose M. , Conroy Simon Paul 
Published: April 2022

AbstractIntroductionThe current service metrics used to evaluate quality in emergency care do not account for specific healthcare outcome goals for older people living with frailty. These have previously been classified under themes of ‘Autonomy’ and ‘Functioning’. There is no person-reported outcome measure (PROM) for older people with frailty and emergency care needs. This study aimed to identify and co-produce recommendations for instruments potentially suitable for use in this population.MethodsIn this systematic review, we searched six databases for PROMs used between 2010 and 2021 by older people living with frailty receiving acute hospital care. Studies were reviewed against predefined eligibility criteria and appraised for quality using the COSMIN Risk of Bias checklist. Data were extracted to map instrument constructs against an existing framework of acute healthcare outcome goals. Instrument face and content validity were assessed by lay collaborators. Recommendations for instruments with potential emergency care suitability were formed through co-production.ResultsOf 9392 unique citations screened, we appraised the full texts of 158 studies. Nine studies were identified, evaluating nine PROMs. Quality of included studies ranged from ‘doubtful’ to ‘very good’. Most instruments had strong evidence for measurement properties. PROMs mainly assessed ‘Functioning’ constructs, with limited coverage of ‘Autonomy’. Five instruments were considered too burdensome for the emergency care setting or too specific for older people living with frailty.ConclusionsFour PROMs were recommended as potentially suitable for further validation with older people with frailty and emergency care needs: COOP/WONCA charts, EuroQol, McGill Quality of Life (Expanded), and Palliative care Outcome Scale.
Validation of the Shock Index, Modified Shock Index, and Shock Index-Paediatric age-Adjusted (SIPA) for predicting length of stay and outcome in children admitted to a paediatric intensive care unit
Authors: Nazar Monish , Kumar Hemanth , Krishnegowda Madhunandan , Unki Praveen , Veerappa Nandakumar , Srinivas Bharath Kumar 
Published: April 2022

Abstract
Background
Maintaining hemodynamic stability requires constant complex interaction between multiple vascular and extravascular factors. There are varieties of parameters that determine the same and few of them are used to predict the hemodynamic instability at earliest. Shock Index (SI), Modified Shock Index (MSI) and Shock Index-Pediatric age-Adjusted (SIPA) have been studied constantly in different clinical settings. They are best non-invasive measures for early prediction in resource poor setting or at community referral centers. We would like to compare the predictive value of each parameter in our tertiary care center.

Methods
It was a retrospective study carried out in PICU of a tertiary care centre and includes data collected from 15 August 2019 to 14 August 2021 over a period of 2 years. We recorded demographic data, age, gender, final diagnosis, outcome, and length of stay in PICU. We compared Outcome (Survived/Expired) and length of stay with SI ≥ 0.7 or < 0.7, MSI ≥ 1.3 or < 1.3 and SIPA > 1.22 or < 1.22 (age 4–6 years) > 1 or < 1 (7–12 years) and > 0.9 or < 0.9 (13–16 years).

Results
This study includes 235 children who were admitted to PICU during study period. The median age was 8 years the median length of stay was 5 days and mortality rate being 11.48% (27). Median SI, MSI were 0.78, 1.6 respectively. 61.70% (145) of patients had SI > 0.7. Median value of SI for septic shock patients was 0.92 on admission. The mortality of the patients with SI > 0.7 was 13.10% (19) and those with MSI > 1.3 was 14.89% (21). Mortality in accordance with SIPA for ages 4–6 years, 7–12 years, and 13–16 years were 15.25% (9), 23% (9) and 19.23% (5) respectively. Basically, SIPA was designed to monitor post trauma cases but in our study we got significant correlation with outcome and length of stay in conditions other than trauma.

Conclusions
The SI, MSI, and SIPA are simple bedside parameters may be used for prioritizing the patients who require strict monitoring on admission to PICU and intervention whenever required. These parameters were best in predicting the severity of sepsis and septic shock in comparison to other diagnosis. SIPA can be generalised for monitoring any high-risk case.
Recent trends in miRNA therapeutics and the application of plant miRNA for prevention and treatment of human diseases
Authors: Saiyed Atiyabanu N. , Vasavada Abhay R. , Johar S. R. Kaid 
Published: April 2022

Abstract
Background
Researchers now have a new avenue to investigate when it comes to miRNA-based therapeutics. miRNAs have the potential to be valuable biomarkers for disease detection. Variations in miRNA levels may be able to predict changes in normal physiological processes. At the epigenetic level, miRNA has been identified as a promising candidate for distinguishing and treating various diseases and defects.

Main body
In recent pharmacology, plants miRNA-based drugs have demonstrated a potential role in drug therapeutics. The purpose of this review paper is to discuss miRNA-based therapeutics, the role of miRNA in pharmacoepigenetics modulations, plant miRNA inter-kingdom regulation, and the therapeutic value and application of plant miRNA for cross-kingdom approaches. Target prediction and complementarity with host genes, as well as cross-kingdom gene interactions with plant miRNAs, are also revealed by bioinformatics research. We also show how plant miRNA can be transmitted from one species to another by crossing kingdom boundaries in this review. Despite several unidentified barriers to plant miRNA cross-transfer, plant miRNA-based gene regulation in trans-kingdom gene regulation may soon be valued as a possible approach in plant-based drug therapeutics.

Conclusion
This review summarised the biochemical synthesis of miRNAs, pharmacoepigenetics, drug therapeutics and miRNA transkingdom transfer.
Ethnobotany of stinging nettle (Urtica simensis Hochst. ex. A. Rich.) in the Oromia region of central and southeastern highlands of Ethiopia
Authors: Shonte Tigist Tadesse , Woldetsadik Kebede 
Published: April 2022

Abstract
Background
Stinging nettle, Urtica simensis, has a wide range of distribution in the highlands of Ethiopia with untapped potential for a sustainable supply of healthy and nutritious food. This study was conducted to document ethnobotanical uses of U. simensis and the associated traditional knowledge of the indigenous people and to identify the factors limiting harvesting and utilization of stinging nettle in North Shewa (R4), Bale and Arsi zones of the Oromia region, central and southeastern highlands of Ethiopia.

Methods
Thirteen districts were purposively selected from the three zones and a total of 130 respondents were sampled, with consideration of gender, age, occupation, and wealth status. Data were collected using semistructured interviews, tour-guided field observations, and focus group discussions. IBM SPSS Statistical software package was used to analyze ethnobotanical data.

Results
Pearson’s chi-square analysis showed that there is a significant association (P < 0.05) between zones and districts in the ethnobotanical knowledge of use categories. Stinging nettle plants have an average use value index of 0.91 with a central role (FL = 44.8%) as a source of food and fodder. Cultural norms and values followed by lack of knowledge and stinginess were the main limiting factor for harvesting and consumption of U. simensis. Cultural norms and values showed significantly higher correlations with the various uses of U. simensis (r2 = 0.931, p < 0.001) indicating the stigma related to stinging nettle being associated as poor man food or famine food were probably a major limiting factor for the utilization of U. simensis.

Conclusions
Future strategic policies should consider U. simensis as a source of food and fodder to fight food insecurity and improve the wellbeing of society. There is a need for local level and countrywide awareness creation, domestication, and future research on value addition and processing for effective utilization of the plant.
Rapid assessment of the temporal function and phenotypic reversibility of neurodevelopmental disorder risk genes in Caenorhabditis elegans
Authors: Kepler Lexis D. , McDiarmid Troy A. , Rankin Catharine H. 
Published: April 2022

ABSTRACT
Recent studies have indicated that some phenotypes caused by decreased function of select neurodevelopmental disorder (NDD) risk genes can be reversed by restoring gene function in adulthood. However, few of the hundreds of risk genes have been assessed for adult phenotypic reversibility. We developed a strategy to rapidly assess the temporal requirements and phenotypic reversibility of NDD risk gene orthologs using a conditional protein degradation system and machine-vision phenotypic profiling in Caenorhabditis elegans. We measured how degrading and re-expressing orthologs of EBF3, BRN3A and DYNC1H1 at multiple periods throughout development affect 30 morphological, locomotor, sensory and learning phenotypes. We found that phenotypic reversibility was possible for each gene studied. However, the temporal requirements of gene function and degree of rescue varied by gene and phenotype. This work highlights the critical need to assess multiple windows of degradation and re-expression and a large number of phenotypes to understand the many roles a gene can have across the lifespan. This work also demonstrates the benefits of using a high-throughput model system to prioritize NDD risk genes for re-expression studies in other organisms.
Εκπαιδευτικό Ντοκιμαντέρ "Achaia Clauss, το Οινόκαστρο της Μαυροδάφνης"
Authors: Γαλανοπούλου Φωτεινή , Κόττα Ελένη , Σοφός Αλιβίζος 
Published: April 2022

Σκοπός του συγκεκριμένου άρθρου είναι η διερεύνηση της εκπαιδευτικής ταινίας με τίτλο: «Achaia Clauss, το Οινόκαστρο της Μαυροδάφνης», η οποία υλοποιήθηκε στο πλαίσιο του ΠΜΣ «Εκπαίδευση με τη χρήση Νέων Τεχνολογιών». Κύριος στόχος της εκπαιδευτικής ταινίας είναι να εμβαθύνουμε στα ιστορικά σημεία και γεγονότα του Οινοκάστρου της Achaia Clauss, αφήνοντας πίσω τα πολυσυζητημένα θέματα για το κομμάτι της οινοποίησης, της εμφιάλωσης και της γενικότερης παραγωγής του κρασιού. Η ταινία συνοδεύεται από εκπαιδευτικές δραστηριότητες που την πλαισιώνουν και θέτουν το παιδαγωγικό πλαίσιο στο οποίο μπορεί αυτή να αξιοποιηθεί. Στη συνέχεια, ακολουθεί ο εκπαιδευτικός σχεδιασμός, καθώς και η διαδικασία παραγωγής και επεξεργασίας του ιστορικού ντοκιμαντέρ. Για τη συγκεκριμένη ταινία θα αναφερθεί ο κατάλληλος εξοπλισμός που χρησιμοποιήθηκε, το αντίστοιχο λογισμικό επεξεργασίας βίντεο, οι ειδικές μαρτυρίες των συμμετεχόντων, ο χρόνος και οι δυσκολίες κατά την παραγωγή της ταινίας. Συμπερασματικά, οι θεατές μέσα από την προβολή της ταινίας και την υλοποίηση των εκπαιδευτικών δραστηριοτήτων θα μάθουν την ιστορία της πρώτης ελληνικής οινοποιίας, θα γνωρίσουν σχετικά παλαιά επαγγέλματα (π.χ. του βαρελοποιού), θα εμβαθύνουν σε σημαντικά γεγονότα γύρω από τη μαυροδάφνη και θα συνδέσουν το κρασί με την αρχαία ελληνική μυθολογία.