bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pilot-Scale Production of Lyophilized Inactivated Rabies Vaccine Candidate in Vero Cells Under Fully Animal Component-Free Conditions Using Microcarrier Technology and Laboratory Animal Trials
Authors: Onen Engin Alp , Bezawada Srinivas 
Published: April 2022

Abstract

The upstream process was carried out in an animal component-free medium on Cytodex 1 microcarriers. Recombinant trypsin is a non-animal derived protease used as an alternative to animal-derived trypsin. To inactivate recombinant trypsin, a soybean trypsin inhibitor (STI) should be added to the medium. A protocol was first tested in T-flasks and then passaged to 500 ml and 3 L spinner flasks. Cell detachment was completed in 10–12 min, and 0.4 g/l STI was added to a 3L spinner and cells were transferred into a 30 L stirred tank bioreactor. On day 5, the cell density had reached its maximum (around 1.8 ×106 cells/ml). Cell infection, at an MOI of 0.3 with serum-free medium conditions, yielded a maximal rabies virus titer of 1.82×107 FFU/mL at 5 days. All cell culture conditions and virus growing kinetics in serum-free media were investigated. In conclusion, Vero cells were grown on Cytodex 1 with serum-free media and a high amount of rabies virus was obtained. Immune response to inactivated rabies virus vaccine candidate was determined in mice challenge. Also, we evaluated inactive rabies vaccine candidate safety, and immunogenicity in mice, sheep, horses, and cattle. We found that no horses, sheep, or cattle were given vaccine IM at 3.2 IU/dose exhibited any clinical sign of disease and all developed high VNA titers (up to 10,03 IU/ml) by 3–4 WPI. After the accelerated stability studies, the lyophilized inactivated rabies vaccine candidate showed enough antigenic potency (2.6 IU/ml) in the mouse challenge test. Also, 18-month long-term stability studies showed enough immune response (1.93 IU/ml) on day 14. The activity of the vaccine candidate showed a good immune response and safety criteria that meet WHO requirements. This is the first pilot-scale mammalian cell-based viral rabies vaccine production study using microcarrier technology reported in Turkey.
Long-term postoperative perfusion status in giant retinal tears: a case report
Authors: QUIROZ-REYES MIGUEL A. , QUIROZ-GONZALEZ ERICK A , QUIROZ-GONZALEZ MIGUEL A. , ALSABER AHMADAD R. , LIMA-GOMEZ VIRGILIO 
Published: April 2022

Abstract
Background
Information about long term perfusional status of patients who have undergone successful surgery for giant retinal tear (GRT) macula-off rhegmatogenous retinal detachment (RRD) is limited.
Purpose
To examine long term perfusional, structural, and functional outcomes in normal control eyes and the eyes treated for different degrees of GRT-associated extensions of RRD.
Methods
One emmetropic normal eye (control), one healthy highly myopic eye (control myopic), and three eyes surgically treated for GRT (surgical), were included in the study for a long-term comparison of study outcomes. The surgical eyes were classified based on the degree of GRT-associated RRD extension as follows: One eye with GRT-associated RRD extension < 180°, one eye with GRT-associated RRD extension between 180°–270°; and one eye with GRT-associated RRD extension > 270°. Structural, functional, and perfusional outcomes were compared with those of control eyes.
Results
All three included eyes were phakic and the condition was monocular. The mean age of the patients was 48.67 ± 8.50 years (range, 39–55 years). All three eyes had GRT macula-off RRD. The mean preoperative time for GRT surgery was 1.2 weeks. The mean pre- and postoperative best corrected visual acuities (BCVA) were 1.87 logMAR and 0.46 logMAR, respectively. The mean postoperative follow-up period was 19.67 ± 5.69 months. Proliferative vitreoretinopathy (PVR) resulted in multiple surgeries in one eye (31.5%). Long-term postoperative optical coherence tomography (OCT) showed abnormal retinal thickness, ellipsoid zone (EZ) disruption, and external limiting membrane (ELM) line discontinuities in one eye. OCT angiography yielded abnormal perfusion indices in the surgically treated eyes.
Conclusions
Our data showed multiple structural alterations in spectral-domain OCT biomarkers. One eye that developed secondary epiretinal membrane (ERM) proliferation showed a significantly improved BCVA after proliferation, and the internal limiting membrane (ILM) were removed. Perfusional findings were correlated with final BCVA. Despite a fully reattached retina without ERM proliferation, GRT-associated RRD has a guarded functional prognosis.
An iterative CNN pruning framework incorporating knowledge distillation
Authors: Xiang Renfei , Li Qiang 
Published: April 2022

Abstract
Convolutional neural network(CNN) with a complex architecture and a large number of layers has high accuracy. However, in some occasions that require high real-time detection, it is not suitable for direct application of these complex neural networks. Thus, some researchers propose pruning to get a sparse model. Current pruning methods mainly focus on static pruning with fixed pruning rate. In this paper we propose an iterative pruning framework to compress model dynamically. To maintain the performance of model, we incorporate knowledge distillation into the whole process. Experimental results prove that our framework can effectively compress the number of parameters of the model with the accuracy not greatly reduced, and the framework has a certain degree of robustness to structural pruning method.
Simulating the behaviour of Glioblastoma Multiforme based on patient MRI during treatments
Authors: Alonzo Flavien , Saad Mazen , Serandour Aurelien 
Published: April 2022

Abstract
Glioblastoma Multiforme is a brain cancer that still shows poor prognosis for patients despite the active research for new treatments. In this work, the goal is to model and simulate the evolution of tumour associated angiogenesis and the therapeutic response to Glioblastoma Multiforme. Multiple phenomena are modelled in order to fit different biological pathways, such as the cellular cycle, apoptosis, hypoxia or angiogenesis.This leads to in a nonlinear system with 4 equations and 4 unknowns: the density of tumour cells, the O2 concentration, the density of endothelial cells and the vascular endothelial growth factor concentration.This system is solved numerically on a mesh fitting the geometry of the brain and the tumour of a patient based on a 2D slice of MRI.We show that our numerical scheme is positive, and we give the energy estimates on the discrete solution to ensure its existence.The numerical scheme uses nonlinear control volume finite elements in space and is implicit in time.Numerical simulations have been done using the different standard treatments: surgery, chemotherapy and radiotherapy, in order to conform to the behaviour of a tumour in response to treatments according to empirical clinical knowledge. We find that our theoretical model exhibits realistic behaviours.
A emergência dos marcadores discursivos note que e vá lá: uma análise funcional centrada no uso
Authors: Machado Teixeira Ana Cláudia 
Published: April 2022

Neste artigo analisamos a emergência dos marcadores discursivos note que e vá lá em contextos específicos cuja vinculação de sentido e forma entre as subpartes constitui uma unidade – uma microconstrução - sob a perspectiva de Traugott e Troudale (2013). A partir do suporte teórico da Linguística Funcional Centrada no Uso, interessa-nos examinar os padrões de uso dessas microconstruções, objetivando detectar os níveis de integração semântico-sintática relacionando-os aos contextos em que estão inseridas. Interessa-nos também examinar os contextos que motivam a emergência de novos usos que passam a articulam crenças e posicionamentos dos produtores textuais. Por fim, nas análises, identificamos a contribuição da abordagem construcional da gramática ao dar tratamento holístico aos dados.
Human Support in App-Based Cognitive Behavioral Therapies for Emotional Disorders: Scoping Review
Authors: Bernstein Emily E , Weingarden Hilary , Wolfe Emma C , Hall Margaret D , Snorrason Ivar , Wilhelm Sabine 
Published: April 2022

Background
Smartphone app–based therapies offer clear promise for reducing the gap in available mental health care for people at risk for or people with mental illness. To this end, as smartphone ownership has become widespread, app-based therapies have become increasingly common. However, the research on app-based therapies is lagging behind. In particular, although experts suggest that human support may be critical for increasing engagement and effectiveness, we have little systematic knowledge about the role that human support plays in app-based therapy. It is critical to address these open questions to optimally design and scale these interventions.

Objective
The purpose of this study is to provide a scoping review of the use of human support or coaching in app-based cognitive behavioral therapy for emotional disorders, identify critical knowledge gaps, and offer recommendations for future research. Cognitive behavioral therapy is the most well-researched treatment for a wide range of concerns and is understood to be particularly well suited to digital implementations, given its structured, skill-based approach.

Methods
We conducted systematic searches of 3 databases (PubMed, PsycINFO, and Embase). Broadly, eligible articles described a cognitive behavioral intervention delivered via smartphone app whose primary target was an emotional disorder or problem and included some level of human involvement or support (coaching). All records were reviewed by 2 authors. Information regarding the qualifications and training of coaches, stated purpose and content of the coaching, method and frequency of communication with users, and relationship between coaching and outcomes was recorded.

Results
Of the 2940 titles returned by the searches, 64 (2.18%) were eligible for inclusion. This review found significant heterogeneity across all of the dimensions of coaching considered as well as considerable missing information in the published articles. Moreover, few studies had qualitatively or quantitatively evaluated how the level of coaching impacts treatment engagement or outcomes. Although users tend to self-report that coaching improves their engagement and outcomes, there is limited and mixed supporting quantitative evidence at present.

Conclusions
Digital mental health is a young but rapidly expanding field with great potential to improve the reach of evidence-based care. Researchers across the reviewed articles offered numerous approaches to encouraging and guiding users. However, with the relative infancy of these treatment approaches, this review found that the field has yet to develop standards or consensus for implementing coaching protocols, let alone those for measuring and reporting on the impact. We conclude that coaching remains a significant hole in the growing digital mental health literature and lay out recommendations for future data collection, reporting, experimentation, and analysis.
Integración de Inputs Positivos y Negativos en Juicios Psicofísicos de Equidad
Authors: Reyes-Contreras Raúl , Santoyo Velasco Carlos 
Published: April 2022

Los modelos de equidad han predicho adecuadamente las ganancias monetarias entre dos empleados hipotéticos que difieren en méritos, sin embargo, han sido incapaces de predecir pérdidas monetarias y condiciones de n>2; se propone la Ecuación General de Distribución de Recursos En Equidad (Función de Equidad) para superar dichas limitantes por lo que el objetivo de la presente investigación consistió en evaluar la generalidad de la Función de Equidad en contextos de pérdidas y ganancias. Participaron voluntariamente 30 estudiantes universitarios de los cuales el 65% fueron mujeres tenían 19.87 años (DE=1.23). En 18 escenarios hipotéticos de acuerdo con un diseño de medidas repetidas (3 niveles de mérito de A) X 3 (niveles de mérito de B) x 2 (Ganancias y Pérdidas) los participantes asignaron montos en ganancias y pérdidas monetarias. Se encontraron reglas de integración aditiva en el campo de las ganancias e indicios de reglas de integración multiplicativa en el campo de las pérdidas. La Ecuación General de Distribución de Recursos en Equidad predice adecuadamente los montos en ganancias y pérdidas. Los datos se discuten a la luz de la Teoría de Integración de Información y las Ciencias Cognitivas y del Comportamiento.
Formulation and Evaluation of Paracetamol Tablets using Coconut Oil as a Binder
Authors: Kulkarni Vaishnavi M. , Babare Shubham B. , Joshi Sanket K. , Walode Sanjay G. , Rudrapal Mithun , Kakade Anand P. , Chatur Vibhavari M. 
Published: April 2022

Tablets are the medicament with suitable excipients. It comprises a mixture of active substances and excipients, usually pressed or compacted from a powder into a solid dose. The aim of this study was to formulate paracetamol tablets using Coconut oil as a binder in different concentrations (1%, 2%, 3%) and evaluate them with basic parameters. The granules manufactured using the binder had good flow property and compressibility. There are various examples of binders that can be used for tablet formulation. Here the efforts were made to formulate the paracetamol tablets by using coconut oil to check whether the tablet can be formulated and the other evaluation parameters for tablets, thereafter. Using coconut oil as a binder in the end gave satisfactory result as we could formulate a tablet with effective concentration of coconut oil with 1%.Theformulated tablets thereafter were evaluated for the characterization for granules to check such as flow property, bulk density, tap density etc.as well as the tablets were also evaluated for disintegration and dissolution as well.
Keywords: Paracetamol, Binder, Dry granulation, Excipients, Direct compression, Disintegration, Dissolution
Toxicological Studies of Hydroethanolic Leaf Extract of Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) on Wistar Rats
Authors: ASSIH Mindédé , Badjabaïssi Essotolom , Bescond Jocelyn , Mouzou Aklesso , Pakoussi Tcha , Sanvee Sabrina Chris Janiba , Yérima Mouhoudine , Diallo Aboudoulatif , Dossou-Yovo Komlan Mawubedjro , Kaboua Komla , Patrick Bois , Potchoo Yao 
Published: April 2022

Introduction: The impact of aromatic plants used in cooking on health is indisputable.
This study aims to evaluate the in vitro cytotoxicity of Artemia salina, acute and the 28 days subchronic toxicity of X. aethiopica dried fruit used like spice in Togo by oral administration of the extract to female Wistar rats.
Results: The A. salina assay showed that the hydroalcoholic extract of X. aethiopica presented any toxicity. The LC50 values of X. aethiopica on brine shrimp were 0.64 ± 0.13 mg/mL. The acute toxicity study revealed neither behavioral disturbances nor death in rats. The lethal dose (DL50) of this extract is greater than 5000 mg/kg body weight. The results of subchronic toxicity showed no significant change in body and organs weight gain in rats at test doses throughout the treatment period. No changes in haematological parameters were observed apart from a significant increase in platelet number at doses of 500 and 1000 mg/kg. Biochemical parameters such as Urea, Creatinine, Triglycerides, Total cholesterol, Serum glutamic pyruvic transaminase (SGPT), C-reactive protein (CRP) and glycemia were not significantly changed. Serum glutamic oxaloacetic transaminase (SGOT) and Alkaline phosphatase (ALP) activities increased at doses of 500/1000 mg/kg and 1000 mg/kg respectively. Creatine Phosphokinase (CPK) activity decrease at the dose of 500 and 1000 mg/kg. Blood electrolytes showed no significant change. The histological sections showed no organ damage.
Conclusions: Aqueous extract of X. aethiopica did not lead to any adverse effects in rats after acute and subchronic treatment at 500 and 1000 mg/kg doses.
Keywords: Xylopia aethiopica, Cytotoxicity, Toxicity, Wistar rats.
Qurṣ-i-Shibb: A review on polyherbal Unani formulation in the management of Kathrat-i-Ṭamth (HMB)
Authors: Najeeb Ghufran , Begum Wajeeha , Sumaiya Syeda 
Published: April 2022

Heavy flow during menstruation is called Menorrhagia or Heavy Menstrual Bleeding, is generally referred to excessive bleeding, either in duration or quantity of blood loss or both (> 8 days and/or >80 ml) at regular intervals. It can be associated with several conditions, including problems with the uterus, hormonal problems, or other conditions. While abundant bleeding may make it difficult to participate in normal daily life at certain times. But currently Several drugs and Hysterectomy is recommended for the treatment of HMB that has different side effects as well as gastrointestinal symptoms, liver disease, obesity, hypoestrogenic state and thromboembolic diseases. In classical texts Qurṣ-i-Shibb is used in the management of Kathrat-i-Ṭamth (Heavy Menstrual Bleeding) as this formulation has pharmacological activity such as ḥābis al-dam (haemostatic or haemostyptic), qābiḍ (astringent), mujafif (siccative), mugharrī (glutinous) and mubarrid (refrigerant). So, this review aims to explore the role of Qurs-i-Shibb in the management of Heavy Menstrual Bleeding. The ingredients of Qurs-i-shibb are shibb-i-yamāni, dam al-khwayn, gulnār each of them 10.5 gram, katīra 21 gram, samagh-i-ʻarabī 7 gram. These drugs having the properties of ḥābis al-dam, qābiz, mujaffif, mugharrī and mubarrid produce vasoconstriction and reduce heavy bleeding. The granules were formulated into 500 mg tablets. Three tablets thrice daily for 5days will help in reducing the bleeding.
Keyword: Kathrat-i-ṭamth; Heavy menstrual bleeding; Qurṣ-ī-Shibb; hemostatic, astringent.
Evaluation of Vitamin D and trace element level in sera of patients with Rheumatoid arthritis
Authors: Ismail Parween Abdulsamad , Mustafa Akam Jasim , Waissi Dlzhin Bahram , Kareem Yusra Sirwan 
Published: April 2022

Objective: The purpose of this research was to assess the serum concentration of (1,25-(OH)2D3) and trace elements in patients with rheumatoid arthritis as compared to healthy individuals.
Methods: This research was performed on 72participants, 37 cases of RA and 35 healthy individuals, with age range 39-67 years. Serum (1,25-(OH)2D3) levels was determined and compared in cases and healthy controls.
Results: The serum (1,25-(OH)2D3) levels were significantly lower in the RA patients (13.24 ± 1.06 ng/ml), when compared to the healthy individuals (26.9 ± 1.7 ng/ml). The examination of data revealed that the levels of (Magnesium, Manganese, Nickel and Rubidium) were remarkably elevated in RA patients when compared to healthy control , serum levels of (Chromium, Cobalt and Germanium) were remarkably decreased in RA patients when compared to healthy control.
Conclusions: (1,25-(OH)2D3) insufficiency is more widespread among patients with RA and may be one of the reasons leading to evolution of rheumatoid arthritis. Supplementation with (1,25-(OH)2D3) may be required both for the avoidance of bone loss besides for painkilling in patients suffering from rheumatoid arthritis. Current results propose that alterations in the serum concentration of trace elements may yield good indication to their role in the pathogenesis of rheumatoid arthritis.
Pengaruh Efikasi Diri Kewirausahaan, Dukungan Sosial, dan Dukungan Edukasi terhadap Intensi Kewirausahaan Sosial pada Mahasiswa Perguruan Tinggi di Jakarta
Authors: Marco Bella Carissa , Selamat Frangky 
Published: April 2022

Penelitian ini bertujuan untuk menguji secara empiris terkait pengaruh efikasi diri kewirausahaan, dukungan sosial dan dukungan edukasi terhadap intensi kewirausahaan sosial. Desain penelitian yang digunakan adalah penelitian kuantitatif dan menggunakan kuesioner untuk pengumpulan data. Jumlah sampel yang diperoleh dengan metode convenience sampling adalah sebanyak 372 mahasiswa. Penelitian ini menggunakan teknik analisis data Structural Equation Modeling – Partial Least Square (SEM-PLS). Hasil penelitian menunjukkan bahwa variabel efikasi diri kewirausahaan dan dukungan sosial memiliki pengaruh positif dan signifikan terhadap intensi kewirausahaan sosial. Variabel dukungan edukasi memiliki pengaruh positif yang lemah dan tidak signifikan terhadap intensi kewirausahaan sosial. This study aims to empirically examine the effect of entrepreneurial self-efficacy, social support and educational support on social entrepreneurial intentions. The research design used was quantitative research and used a questionnaire for data collection. The number of samples obtained by the convenience sampling method amounted to 372 students. This study uses Structural Equation Modeling – Partial Least Square (SEM-PLS) as the data analysis techniques. The results showed that entrepreneurial self-efficacy and social support variables had a positive and significant effect on social entrepreneurial intentions. The educational support variable has a weakly positive and insignificant effect on social entrepreneurship intentions.
Pengaruh Budaya Organisasi dan Lingkungan Kerja terhadap Kepuasan Kerja Karyawan PT. MMU Jakarta
Authors: Dameria Lusiana , Ekawati Sanny 
Published: April 2022

Tujuan penelitian ini adalah untuk mengetahui pengaruh budaya organisasi dan lingkungan kerja terhadap kepuasan kerja. Desain penelitian yang digunakan adalah penelitian kausal dengan metode kuantitatif. Pengumpulan data menggunakan kuesioner dalam bentuk Google form. Ukuran sampel yang digunakan dalam penelitian ini sebanyak 70 karyawan tetap PT. MMU, di Jakarta. Metode sampling yang digunakan yaitu non-probability sampling dengan teknik purposive sampling. Penelitian ini menggunakan perangkat lunak SmartPLS Versi 3 dalam menganalisis data. Variabel budaya organisasi menggunakan indikator inovasi dan pengambilan resiko, memperhatikan hal rinci, orientasi hasil, orientasi karyawan, orientasi tim, keagresifan, dan stabilitas. Variabel lingkungan kerja menggunakan indikator lingkungan kerja fisik dan non fisik. Variabel kepuasan kerja menggunakan indikator pekerjaan itu sendiri, gaji, promosi, supervisi, dan rekan kerja. Hasil penelitian ini menunjukkan bahwa budaya organisasi dan lingkungan kerja berpengaruh positif dan signifikan terhadap kepuasan kerja. This study was conducted to determine the effect of organizational culture and work environment towards job satisfaction. The research design used in this study was causal research with quantitative method. The data was collected by distributing questionnaire in google form. The sample size used in this study were 70 permanent employees at PT. MMU, in Jakarta. The sampling method used in this study was non-probability sampling with purposive sampling technique. This study used software SmartPLS Version 3.0 in analyzing the data. Indicators of organizational culture were innovation and risk taking, attention to detail, result orientation, employee orientation, team orientation, aggressiveness, and stability. Indicators of work environment were physical and non-physical work environment. Indicators of job satisfaction were the job itself, salary, promotion, supervision, and co-workers. The results of this study indicated that organizational culture and work environment had a positive and significant effect towards job satisfaction.