bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo quantitative high-throughput screening for drug discovery and comparative toxicology
Authors: Dranchak Patricia K. , Oliphant Erin , Queme Bryan , Lamy Laurence , Wang Yuhong , Huang Ruili , Xia Menghang , Tao Dingyin , Inglese James 
Published: August 2022

AbstractQuantitative high-throughput screening (qHTS) evaluates the pharmacology of drug and investigational agent libraries for potential therapeutic uses, toxicological risk assessment, and increasingly for academic chemical tool discovery. Phenotypic HTS assays aim to interrogate molecular pathways and networks, often relying on cell culture systems, historically with less emphasis on multicellular organisms. C. elegans has served as a powerful eukaryotic model organism for human biology and disease by virtue of genetic conservation and experimental tractability. Here we describe a paradigm to enable C. elegans in qHTS using 384-well microtiter plate laser scanning cytometry. GFP-expressing organisms are used to reveal phenotype-modifying structure-activity relationships to guide subsequent life stages and proteomic analysis. E. coli bacterial ghosts, a non-replicating nutrient source, allow compound exposures over 7-days spanning two life cycles to mitigate complications from bacterial overgrowth. We demonstrate the method with a library composed of anti-infective agents, or molecules of general toxicological concern. Each was tested in 7-point titration to assess the feasibility of nematode-based in vivo qHTS, and examples of follow-up strategies were provided to study organism-based chemotype selectivity and subsequent network perturbations having a physiological impact. We anticipate a broader application of this qHTS-coupled proteomics approach will enable the analysis of C. elegans orthologous transgenic phenotypes of human pathologies to facilitate drug and probe profiling from high-impact chemical libraries for a range of therapeutic indications and study of potential toxicological signatures.Graphic Abstract
Cell Atlas at Single-Nuclei Resolution of the Adult Human Adrenal Gland and Adrenocortical Adenomas
Authors: Altieri Barbara , Secener A. Kerim , Sai Somesh , Fischer Cornelius , Sbiera Silviu , Arampatzi Panagiota , Herterich Sabine , Landwehr Laura-Sophie , Vitcetz Sarah N. , Braeuning Caroline , Fassnacht Martin , Ronchi Cristina L. , Sauer Sascha 
Published: August 2022

AbstractThe human adrenal gland is a complex endocrine tissue. Developmental studies on this tissue have been limited to animal models or human foetus. Here, we present a cell atlas analysis of the adult human normal adrenal gland, combining single-nuclei RNA sequencing and spatial transcriptome data to reconstruct adrenal gland development and tumourigenesis. We identified two populations of potential progenitor cells resident within the adrenal cortex: adrenocortical progenitors NR2F2+-ID1+cells, located within and underneath the capsule, and medullary progenitors SYT1+-CHGA−cells, located in islets in the subcapsular region. Using pseudotime analyses, we provided evidence of the centripetal nature of adrenocortical cell development and of the essential role played by the Wnt/β-catenin pathway in the adrenocortical self-renewal. By comparing transcriptional profiles of cells of normal adrenal glands and adrenocortical adenomas we revealed a high heterogeneity with six adenoma-specific clusters. Overall, our results give insights into adrenal plasticity and mechanisms underlying adrenocortical tumourigenesis.
Discovery of phage determinants that confer sensitivity to bacterial immune systems
Authors: Stokar-Avihail Avigail , Fedorenko Taya , Garb Jeremy , Leavitt Azita , Millman Adi , Shulman Gabriela , Wojtania Nicole , Melamed Sarah , Amitai Gil , Sorek Rotem 
Published: August 2022

AbstractOver the past few years, numerous anti-phage defense systems have been discovered in bacteria. While the mechanism of defense for some of these systems is understood, a major unanswered question is how these systems sense phage infection. To systematically address this question, we isolated 192 phage mutants that escape 19 different defense systems. In many cases, these escaper phages were mutated in the gene sensed by the defense system, enabling us to map the phage determinants that confer sensitivity to bacterial immunity. Our data identify specificity determinants of diverse retron systems and reveal phage-encoded triggers for multiple abortive infection systems. We find general themes in phage sensing and demonstrate that mechanistically diverse systems have converged to sense either the core replication machinery of the phage, phage structural components, or host takeover mechanisms. Combining our data with previous findings, we formulate key principles on how bacterial immune systems sense phage invaders.
Borderline personality disorder following resection of large sagittal sinus meningioma is evidenced by a self-other voice discrimination task: a case report
Authors: Orepic Pavo , Iannotti Giannina Rita , Haemmerli Julien , Goga Cristina , Park Hyeong-Dong , Betka Sophie , Blanke Olaf , Michel Christoph M. , Bondolfi Guido , Schaller Karl 
Published: August 2022

ABSTRACTPersonality changes following neurosurgical procedures pose a major concern for patients and remain poorly understood both by clinicians and neuroscientists. Here we report a case of a female patient in her 50s who underwent resection of a large sagittal sinus meningioma with bilateral extension, including resection and ligation of the superior sagittal sinus, that resulted in borderline personality disorder and symptoms resembling the Gastaut-Geschwind syndrome. Clinical observations were further reflected and experimentally quantified with a series of behavioral and neuroimaging tasks assessing self-other voice discrimination, one of the established markers for self-consciousness. In all tasks, the patient consistently confused self- and other voices – i.e., she misattributed other-voice stimuli to herself and self-voice stimuli to others. Moreover, behavioral findings were corroborated with scalp EEG results. Specifically, the same EEG microstate, that was in healthy participants associated with hearing their own voice, in this patient occurred more often for other-voice stimuli. We hypothesize that the patient’s preexisting psychological problems were significantly aggravated by postoperative decompensation of a fragile steady-state combination of direct frontal lobe compression and preoperative development of a large venous collateral hemodynamic network that followed gradual occlusion of the superior sagittal sinus. Resection of the sagittal sinus together with the tumor impacted venous drainage of brain areas associated with self-consciousness. These findings are of high relevance for developing experimental biomarkers of post-surgical personality alterations.
Structural basis of CBP/p300 recruitment by the microphthalmia-associated transcription factor
Authors: Brown Alexandra D. , Vergunst Kathleen L. , Branch Makenzie , Blair Connor M. , Dupré Denis J. , Baillie George S. , Langelaan David N. 
Published: August 2022

ABSTRACTThe microphthalmia-associated transcription factor (MITF) is a master regulator of the melanocyte cell lineage. Aberrant MITF activity can lead to multiple malignancies including skin cancer, where it modulates the proliferation and invasiveness of melanoma. MITF-dependent gene expression requires recruitment of transcriptional co-activators such as CBP/p300, but details of this process are not fully defined. Here, we investigate the structural and functional interaction between the MITF N-terminal transactivation domain (MITFTAD) and CBP/p300. A combination of pulldown assays and nuclear magnetic resonance spectroscopy determined that MITF binds both TAZ1 and TAZ2 domains of CBP/p300 with high affinity. The solution-state structure of the MITFTAD:TAZ2 complex reveals that MITF interacts with a hydrophobic surface of TAZ2, while remaining relatively dynamic. Peptide array and mutagenesis experiments determined that an acidic motif is integral to the MITFTAD:TAZ2 interaction and is necessary for transcriptional activity of MITF. Peptides that bind to the same surface of TAZ2 as MITFTAD, such as the adenoviral protein E1A, are capable of displacing MITF from TAZ2 and inhibiting transactivation. These results provide mechanistic insight into co-activator recruitment by MITF that are fundamental to our understanding of MITF targeted gene regulation and melanoma biology.
The fate of accounting for public governance development
Authors: Grossi Giuseppe , Argento Daniela 
Published: August 2022

PurposeThe purpose of this paper is to explain how public sector accounting has changed and is changing due to public governance development.Design/methodology/approachThis paper conducts a traditional literature review based on selected studies in the fields of accounting, public administration and management. The aim of the review is to explain how diverse forms of public governance influence the fate of public sector accounting, including accountability, performance measurement, budgeting and reporting practices.FindingsPublic governance is developing into more inclusive but also complex forms, resulting in network, collaborative and digital governance. Consequently, the focus and practices of public sector accounting have changed, as reflected in new types of accountability, performance measurement, budgeting and reporting practices.Research limitations/implicationsDrawing upon literature from different fields enables a deeper understanding of the changes in public sector accounting. Nevertheless, the intention is not to execute a systematic literature review but to provide an overview and resolve the scattered body of knowledge generated by previous contributions. The areas of risk management and auditing were not included and deserve further attention.Originality/valueThis paper discusses the need to continually redefine and reassess public sector accounting practices, by recognising the interdependencies between different actors, citizens and digital technologies.
Experiences of equine assisted therapy for females with autism spectrum disorders
Authors: Warner Katie , Keville Saskia , Hockley Jemma , Ludlow Amanda 
Published: August 2022

Purpose
This research indicates females with autism spectrum disorders (ASDs) have a diverse clinical presentation compared to males. Furthermore, females with ASD are often diagnosed later and typically experience greater levels of mental health difficulties. Evidence suggests that clinic-based verbal interventions for ASD have limited efficacy; therefore, alternative therapies, such as equine-assisted therapies (EATs), are gaining recognition. The purpose of this study was to directly explore the experiences of females with an ASD who have undertaken EAT.

Design/methodology/approach
Five female participants with a diagnosis of ASD were recruited from two equine therapy centres. Participants were aged between 15 and 30 years and undertook semi-structured interviews, which were analysed using interpretative phenomenological analysis.

Findings
Three superordinate themes emerged: the difficult experience of the social world, the process of EAT and the emotional impact of horses.

Originality/value
Directly exploring the experiences of females with ASD highlights benefits from engaging therapeutically with horses, building confidence and independence to transferring this into more effective social communication with other people. Offering emotion-focused therapeutic complementary interventions for females with ASD should be forefronted to help remediate the impact of difficult and sometimes traumatic earlier experiences in the social world. This requires increased funding for EAT, combined with larger-scale research projects to evaluate this.
Addressing sustainability during and post-COVID-19 pandemic crisis: a literature review and bibliometric analysis to explore the future avenues
Authors: Devi Yashoda , Srivastava Abhishek 
Published: August 2022

PurposeThis paper aims to identify the current research trends in sustainability through an extensive literature review and propose future research agenda under the ambit of the ongoing coronavirus disease 2019 (COVID-19) pandemic. Specifically, the present study aims to uncover the current state of the literature and thereby develop an understanding of how the pandemic has impacted the dimensions of sustainability.Design/methodology/approachTo achieve the objective, the authors adopted a five-step literature review process, combined with bibliometric and network analysis. The authors also considered news articles and reports of international organizations to comprehensively achieve the research objective.FindingsThe study results show how the pandemic has impacted the social, economic and environmental dimensions of sustainability. The results also highlight the list of authors, countries and institutions that have proactively worked towards eliminating the disruption caused by the COVID-19 pandemic.Research limitations/implicationsThe study provides an in-depth understanding of the scholarly contributions to the field of sustainability since the COVID-19 outbreak. The study is useful for policymakers and businesses interested in understanding how the pandemic has impacted the dimensions of sustainability and possible mitigation strategies. Furthermore, the study also provides future research directions in the intersection of pandemic and sustainability dimensions. Specific research questions (RQs) are also proposed to help future research.Originality/valueTo the best of the authors' knowledge, this study is a pioneer attempt to provide a comprehensive understanding of the existing and rapidly growing literature on COVID-19 and its relationship with the various dimensions of sustainability.
Guiding new Middle managers in developing effective communication practices
Authors: Schraeder Mike 
Published: August 2022

Purpose
The purpose of the article is to provide practical recommendations and insights for leaders and/or organizations to consider as they guide new middle managers in their efforts to develop a preliminary foundation for effective communication practices.

Design/methodology/approach
The article is framed within the context of new middle managers. Emphasis is placed on providing examples of ways that leaders and organizations can assist or guide new middle managers in developing a foundation for preliminary communication practices.

Findings
Guidance from upper-level leaders related to the foundation should include, among other things, an overview of expectations, organizational goals, and vision. The article also includes some examples of preliminary communication practices that upper-level leaders might suggest for consideration by new middle managers. The examples include, preferred communication channels, preferences related to responsiveness, preferred characteristics of electronic communication, being intentional, and promoting balance/wellbeing.

Originality/value
Middle managers are important to the success of organizations. The role of middle managers encompasses several requisite skill sets. Among those skill sets is the need for effective communication. The article approaches the topic of effective communication in the context of new middle managers, with an emphasis on leader/organizational guidance that can contribute to a preliminary foundation for effective communication practices. The foundation and examples can then serve as a platform for ongoing development of a more comprehensive communication plan.
Design and implementation of a relational model of risk communication
Authors: Lejano Raul P. , Haque Ahmadul , Kabir Laila , Rahman Muhammad Saidur , Pormon Miah Maye , Casas Eulito 
Published: August 2022

PurposeThe intent of the work is to go beyond the conventional model of disaster risk prevention, where community residents are objects of risk communication initiatives, and develop and implement a relational model of risk communication wherein they are active agents of knowledge transfer.Design/methodology/approachThe relational model of risk communication translates risk knowledge into narrative forms that community members can share. The article discusses the conceptual basis of the model and, then, describes how it has been pilot tested and implemented in the field. Evaluation of the pilot tests consist of pre- and post-surveys comparing control and test groups.FindingsEncouraging results have been seen among vulnerable communities, such as residents in a refugee camp and schoolchildren in a storm surge vulnerable town. These outcomes support the idea that the relational approach can empower residents to be active agents of risk communication.Originality/valueThe relational model taps into the knowledge and agency of community.
Effect of knowledge resources on innovation and the mediating role of dynamic capabilities: case of medical tourism sector in Iran
Authors: Edgar Gerry , Kharazmi Amirali , Behzadi Sedigheh , Kharazmi Omid Ali 
Published: August 2022

PurposeThis research is an empirical study that addresses whether knowledge resources impact on, or do not impact on, innovation development and if this impact is mediated by dynamic capabilities in the medical tourism sector in Mashhad city, Iran.Design/methodology/approachA quantitative research methodology was applied and questionnaires were used for data collection in this study. A total of 108 questionnaires were collected of which 102 questionnaires were valid. Data were analyzed using structural equation modelling technique.FindingsEmpirical evidence obtained from the study reveals that the dynamic capability of learning plays a significant role in transforming knowledge resources into innovation in the medical tourism sector. The mediating role of coordinating capability in the relationship between explicit and tacit knowledge and innovation is considerable and it influences human capital, as well. Sensing capability also exhibits some degree of a mediating role; however, integrating capability is not influential and its role in transforming explicit knowledge to innovation is rejected.Originality/valueMost studies on innovation in medical tourism focused on market and its typology, and neglected the role of knowledge resources and dynamic capabilities. The current study bridges this gap and thus contributes to the scientific literature.
New conceptual tools for be(com)ing antiracist (teacher) educators at PWIs
Authors: Clark Caroline T. , Skrlac Lo Rachel , Boyd Ashley , Cook Michael , Crawley Adam , Rish Ryan M. 
Published: August 2022

PurposeThis study aims to share the development of new conceptual tools, which merge theories of critical whiteness studies (CWS), epistemic injustice and abolitionist teaching, applying them to the discourse of pre- and in-service teachers across the predominantly white institutions (PWIs) as they discuss antiracist teaching through the book Stamped and a series of online discussions.Design/methodology/approachThis qualitative, collaborative practitioner inquiry derived data from video-recorded, online discussions, interviews and weekly research meetings. Critical discourse analysis revealed theoretical gaps and prompted the integration of additional theories, resulting in new conceptual tools, which are applied here to both “in the moment” exchanges between participants and individuals’ reflections in interviews.FindingsApplying new conceptual tools to discussions of whiteness and race revealed how epistemic harm, microresistance and epistemic justice emerge in talk along with the importance of cultivating critical vigilance among antiracist educators.Originality/valueThis study elucidates how merging the conceptual frameworks of CWS, epistemic injustice and abolitionist teaching provides new tools for interrogating antiracism relative to whiteness in participants’ and researchers’ experiences. It challenges teacher educators, particularly at PWIs, to recognize how epistemic harm may be inflicted on students of color when centering whiteness in teacher education.
Socio-ecological risks management dynamic simulation in megaproject development of the Edinburgh Tram Network
Authors: Boateng Prince , Omotayo Temitope , Osunsanmi Temidayo , Ekundayo Damilola 
Published: August 2022

PurposeThe inherent risks and their interactive impacts in megaproject development have been found in numerous cases worldwide. Although risk management standards have been recommended for the best practice in engineering construction projects, there is still a lack of systematic approaches to describing the interactions. Interactions such as social, technical, economic, ecological and political (STEEP) risks have complex and dynamic implications for megaproject construction. For a better understanding and effective management of megaprojects such as the Edinburgh Tram project, the dynamic interaction of concomitant risks must be studied.Design/methodology/approachA systems dynamic methodology was adopted following the comprehensive literature review. Documentary data were gathered from the case study on Tram Network Project in Edinburgh.FindingsA casual loop of typical evolution of key indicators of risks was then developed. A hypothesised model of social and ecological (SE) risks was derived using the system dynamics (SD) modelling technique. The model was set up following British Standards on risk management to provide a generic tool for risk management in megaproject development. The study reveals that cost and time overruns at the developmental stage of the case project are caused mainly by the effects of interactions of risk factors from the external macro project environment on a timely basis.Originality/valueThis article presented a model for simulating the socio-ecological risk confronting the management and construction of megaprojects. The use of SD provided the opportunity to explain the nature of all risks, particularly the SE risks in the past stages of project development.
“Let’s manage the stressor today” exploring the mental health response to forced migrants in Johannesburg, South Africa
Authors: Walker Rebecca , Vearey Jo 
Published: August 2022

Purpose
In South Africa, the majority of the population struggles to access care and support for mental health challenges. Drawing on challenges faced by asylum seekers and refugees in the urban margins of Johannesburg, this paper aims to explore the relationship between migration and mental health through a lens of heightened vulnerability, precarious urban spaces and unmet basic needs.

Design/methodology/approach
Remote interviews were conducted with respondents working in the mental health-care sector (public and private) and with migrant communities in Johannesburg. Respondents were identified via purposive sampling and interviews were conducted in English. Key findings were identified using thematic analysis.

Findings
Effective responses to asylum seekers and refugees facing mental health challenges are based on an understanding of context, of crisis and of the need to meet basic needs such as paying rent, finding employment and providing for families. These “daily stressors” not only compound “extreme traumatisation” but are a form of trauma in and of itself.

Originality/value
This paper shows how alternative responses determined by an understanding of context, of crisis and of the need to meet basic needs provide critical and potentially far-reaching interventions. Locating trauma in the unmet needs, precarious urban spaces and marginalisation opens up space to further question the ways that migration and mental health shape and reshape one another.