bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy
Authors: Yu Jason S.L. , Heineike Benjamin M. , Hartl Johannes , Correia-Melo Clara , Aulakh Simran Kaur , Lehmann Andrea , Lemke Oliver , Agostini Federica , Lee Cory T. , Demichev Vadim , Messner Christoph B. , Mülleder Michael , Ralser Markus 
Published: March 2022

AbstractThe assimilation, incorporation, and metabolism of sulfur is a fundamental process across all domains of life, yet how cells deal with varying sulfur availability is not well understood. We studied an unresolved conundrum of sulfur fixation in yeast, in which an organosulfur-auxotrophy caused by deletion of homocysteine synthase Met17p is overcome when cells are inoculated at high cell density. We discovered that an uncharacterized gene YLL058Wp, herein named Hydrogen sulfide utilizing-1 (HSU1), acts as a homocysteine synthase and allows the cells to substitute for Met17p by re-assimilating hydrosulfide ions leaked frommet17Δcells into O-acetyl-homoserine and forming homocysteine. Our results show that cells can cooperate to achieve sulfur fixation, indicating that the collective properties of microbial communities facilitate their basic metabolic capacity.SummarySulfur limitation activates a dormant hydrogen sulfide fixation route via a novel homocysteine synthase Hsu1p (YLL058Wp).
Molecular architecture of the TasA biofilm scaffold in Bacillus subtilis
Authors: Böhning Jan , Ghrayeb Mnar , Pedebos Conrado , Abbas Daniel K. , Khalid Syma , Chai Liraz , Bharat Tanmay A. M. 
Published: March 2022

AbstractMany bacteria in nature exist in multicellular communities termed biofilms. Cells within biofilms are embedded in a primarily self-secreted extracellular polymeric matrix that provides rigidity to the biofilm and protects cells from chemical and mechanical stresses. In the Gram-positive model biofilm-forming bacterium Bacillus subtilis, TasA is the major protein component of the biofilm matrix, where it has been reported to form functional amyloid fibres contributing to biofilm structure and stability. The structure of TasA fibres, however, and how fibres scaffold the biofilm at the molecular level, is not known. Here, we present electron cryomicroscopy structures of TasA fibres, which show that rather than forming amyloid fibrils, TasA monomers assemble into filaments through donor strand complementation, with each subunit donating a β-strand to complete the fold of the next subunit along the filament. Combining electron cryotomography, atomic force microscopy, and mutational studies, we show how TasA filaments congregate in three dimensions to form abundant fibre bundles that are essential for B. subtilis biofilm formation. This study explains the previously observed biochemical properties of TasA and shows, for the first time, how a bacterial extracellular globular protein can assemble from monomers into β-sheet-rich fibres, and how such fibres assemble into bundles in biofilms. We establish a hierarchical, atomic-level assembly mechanism of biofilm scaffolding that provides a structural framework for understanding bacterial biofilm formation.
Real-time denoising of fluorescence time-lapse imaging enables high-sensitivity observations of biological dynamics beyond the shot-noise limit
Authors: Li Xinyang , Li Yixin , Zhou Yiliang , Wu Jiamin , Zhao Zhifeng , Fan Jiaqi , Deng Fei , Wu Zhaofa , Xiao Guihua , He Jing , Zhang Yuanlong , Zhang Guoxun , Hu Xiaowan , Zhang Yi , Qiao Hui , Xie Hao , Li Yulong , Wang Haoqian , Fang Lu , Dai Qionghai 
Published: March 2022

AbstractA fundamental challenge in fluorescence microscopy is the inherent photon shot noise caused by the inevitable stochasticity of photon detection. Noise increases measurement uncertainty, degrades image quality, and limits imaging resolution, speed, and sensitivity. To achieve high-sensitivity imaging beyond the shot-noise limit, we provide DeepCAD-RT, a versatile self-supervised method for effective noise suppression of fluorescence time-lapse imaging. We made comprehensive optimizations to reduce its data dependency, processing time, and memory consumption, finally allowing real-time processing on a two-photon microscope. High imaging signal-to-noise ratio (SNR) can be acquired with 10-fold fewer fluorescence photons. Meanwhile, the self-supervised superiority makes it a practical tool in fluorescence microscopy where ground-truth images for training are hard to obtain. We demonstrated the utility of DeepCAD-RT in extensive experiments, including in vivo calcium imaging of various model organisms (mouse, zebrafish larva, fruit fly), 3D migration of neutrophils after acute brain injury, and 3D dynamics of cortical ATP (adenosine 5’-triphosphate) release. DeepCAD-RT will facilitate the morphological and functional interrogation of biological dynamics with minimal photon budget.
Ecological consciousness and sustainable purchase behavior: the mediating role of psychological ownership
Authors: Mishra Sita , Malhotra Gunjan , Chatterjee Ravi , Kareem Abdul Waheed 
Published: March 2022

PurposeThis research focuses on the consumption behavior of young consumers by examining their ecological consciousness and several self-oriented and psychological variables (fashion-orientation, frugality and psychological ownership toward the environment) that may trigger their sustainable purchase behavior. The research used “Psychological Ownership Theory” to examine sustainable purchase behavior in the context of apparel consumption.Design/methodology/approachTo gather data, a multi-method approach is used, including a scenario-based choice experiment (n = 62) and a structured survey (n = 338) using an online self-administered questionnaire. Data analysis was done using SPSS AMOS version 25 and PROCESS SPSS macro.FindingsThe results of the two studies revealed the mediation effect of “psychological ownership toward the environment” between ecological consciousness and sustainable purchasing behavior. Furthermore, fashion-orientation was found to be a significant moderating variable influencing sustainable purchase behavior; however, the impact of frugality was insignificant.Originality/valueThis research is one of the first that examined the mediation impact of psychological ownership of the environment in sustainable purchasing behavior using multi-method. The research focuses on the ecological consciousness of young consumers that develop psychological ownership.
Exploring heterogeneity in meat consumption and eating out by using a latent class model
Authors: Ritzel Christian , Mann Stefan 
Published: March 2022

PurposeWhile it is incontestable that eating in restaurants leads to a higher energy intake than eating at home, this paper explores the even more environmentally relevant connection between meat intake and the location of eating.Design/methodology/approachBased on secondary data from the US National Health and Nutrition Examination Survey (NHANES), the authors apply a latent class model (LCM), combining latent profile analysis (LPA) and regression analysis. Different (latent) consumer classes are modeled based (1) on share of meat consumption and (2) share of eating out by means of LPA, while class-specific socio-demographic characteristics are estimated by means of ordered logistic regression.FindingsResults of the LPA reveal four (latent) consumer classes with regard to the share of meat consumption and the share of eating out. One class consists mostly of male meat lovers with a high share of eating out, which, however, only represents 7% of the sample. A much larger class represents an affluent social group that consumes the majority of food outside of the home but does not consume significantly more meat than the large group of moderates who mostly eat at home. The fourth class mostly consists of children with a very low intake of meat.Originality/valueBy applying a LCM, the authors shed some light on the relation between meat consumption and eating out. The authors demonstrated that commonly assumed relations, such as men eating more meat than women, do not necessarily apply. Similar findings apply to factors potentially influencing meat consumption, such as education, marital status and income.
Critical factors of construction workers' career promotion: evidence from Guangzhou city
Authors: Xie Lin-lin , Luo Ziyuan , Zhao Xianbo 
Published: March 2022

PurposeThis study aims to build a framework of the influencing factors of construction workers' career promotion and identifies the critical determinants so as to propose suggestions for the government and enterprises to offer construction workers a path for career promotion.Design/methodology/approachIn line with the theory of human resources, such as Herzberg's two-factor theory, this study constructs a theoretical framework that affects the career promotion of construction workers. Using evidence from Guangzhou city, valid data provided by 464 workers from 50 sites were collected by a questionnaire survey, and the significance test on the influencing factors of construction workers' career promotion was taken by binary logistic regression.FindingsThe overall career development of construction workers in Guangzhou is worrying. The binary logistic regression indicates that age, working years, type of work, career development awareness, legal awareness, professional mentality, vocational psychological training and career development path are critical factors that affect construction workers' career promotion.Originality/valueThis study for the first time explores the career promotion of frontline construction workers. Specifically, it identifies the critical factors that affect the career promotion of workers and thus lays a foundation for further research and the promotion and continuous and healthy development of the construction industry. Thus, this study is original and has theoretical and practical significance.
Understanding the accessibility of retail mobile banking during the COVID-19 pandemic
Authors: Naeem Muhammad , Ozuem Wilson , Ward Philippa 
Published: March 2022

PurposeThis study offers an understanding of vulnerable populations' experiences of actual use of mobile banking and their expectations of mobile banking (MB).Design/methodology/approachData were generated from MB customers and bankers using online reviews, focus groups and semi-structured interviews, as a mix of methods and sources can provide rich and in-depth understanding.FindingsThe affordance of MB for vulnerable populations is explained in four concepts: meaning, material, competency and usability. Recommendations that could further engage and improve the service quality of MB apps for vulnerable populations include customization and personalization of services, access to the digital health data of members of vulnerable populations, audio-based option selection and touchscreen options, and enhancement of service and performance standards.Research limitations/implicationsIt is suggested that retail bankers should improve the service quality and performance of their MB apps by considering the recommendations drawn from vulnerable people's experiences. This study discusses implications for retailers.Originality/valueThis study applied social practice theory and affordance of technology theory to understand how those in vulnerable populations experienced MB apps; the results could be used to improve the accessibility, performance and service quality of MB apps.
Trust and beliefs about robots in Latin America
Authors: Marroquin Andres , Saravia Antonio 
Published: March 2022

PurposeThe goal of this paper is to study the factors that determine individuals' beliefs about robots in Latin America. The authors highlight the role of interpersonal trust in shaping these beliefs.Design/methodology/approachThe authors use data from 2018 Latinobarómetro survey in 18 Latin American countries. The analysis includes an empirical strategy that reduces overt bias and weighs hidden bias.FindingsRobots are not generally supported or positively perceived in Latin America. However, people are less negative about robots when they (1) trust others, (2) are male, (3) are single, (4) are more educated, (5) support democracy and (6) favor free imports. Interpersonal trust is the most consistent covariate. The results provide support to the idea that trusting robots can be the result of trusting humans working in the technological sector (Coeckelbergh, 2012).Research limitations/implicationsThis study faces some limitations. The first one relates to the standard caveats associated with the use of survey data – interviewees may not express their true beliefs. Another limitation is that this study examines data for only one year (the 2018 Latinobarómetro survey). Finally, although propensity score matching allow to control for endogeneity due to observable characteristics and the authors perform a sensitivity test regarding hidden characteristics, the nature of the data does not allow to guarantee that there is a causal effect from interpersonal trust to beliefs about robots.Practical implicationsThe authors find that the positive relationship between trust and beliefs about robots is particularly strong in the area of health. Medical companies could consider these findings when designing and promoting robots.Social implicationsNegative views about robots may delay the adoption of new technology in the region. To facilitate the adoption of new technologies, stakeholders should pay attention to the prevalent levels of interpersonal trust in society. This is especially important in developing countries.Originality/valueTo the authors’ knowledge this is the first study that empirically links beliefs about robots and interpersonal trust.
Impact of accounting on reorganization success: empirical evidence from bankrupt SMEs
Authors: Kuttner Michael , Mayr Stefan , Mitter Christine , Duller Christine 
Published: March 2022

Purpose
Small- and medium-sized enterprises (SMEs) often lack adequate accounting systems and may even fail because of accounting inefficiencies. Indeed, accounting can mitigate the course of a crisis and support a troubled SME’s turnaround. Its impact on reorganization success, however, has scarcely been researched so far. Therefore, this paper aims to examine the effects of several accounting parameters, namely, the quality of accounting systems, quality of early warning systems, formal planning, the standard of financial accounting and reorganization planning on the short- and long-term success of court-supervised reorganization.

Design/methodology/approach
The impact of accounting on reorganization success is investigated in a sample of all SME bankruptcy cases with ten or more employees (n = 117) in Upper Austria in 2012 including data for short-term survival (in 2016) and long-term survival (in 2019).

Findings
This study found evidence that the general quality of accounting systems, the quality of early warning systems and written reorganization plans positively influence the outcomes of the analyzed court-supervised reorganizations of SMEs. In particular, the existence of a reorganization plan significantly increases the short- and long-term reorganization success by ensuring the efficient and effective use of resources in the reorganization process.

Practical implications
This study should increase the awareness of SMEs’ owner managers, consultants, creditors and legislators for the importance of accounting in the context of reorganization. The fact that the effect of accounting on reorganization success is less pronounced in the long-term view indicates the necessity of increasing the strategic focus in SMEs’ accounting instruments.

Originality/value
This study provides new evidence on the impact of specific accounting parameters on the short- and long-term success of the court-supervised reorganization of SMEs. Furthermore, this study points out the high relevance of reorganization plans for SMEs.
Infrastructure design stage considerations for environmental sustainability in Zambia
Authors: Zulu Ephraim , Zulu Sambo Lyson , Chabala Mwansa , Kavishe Neema , Chifunda Charles , Musonda Innocent 
Published: March 2022

Purpose
While previous studies have highlighted the importance of incorporating environmental sustainability in building designs, there is a paucity of studies that assess the extent to which design teams in developing countries consider environmental sustainability at the building design stage. Therefore, using Zambia as a case study, this study examined the extent to which infrastructure design teams in a developing country consider environmental sustainability at the design stage.

Design/methodology/approach
The study used a qualitative research approach using structured interviews because there are hardly any studies which have explored the extent to which designers incorporate environmental sustainability in infrastructure designs in developing countries. The data is analysed thematically using the ATLAS.ti software.

Findings
The results show that environmental sustainability is not an important design consideration because it is secondary to functional, technical and aesthetic considerations. Environmental considerations are also made in an ad hoc manner and when it is cost-effective for the project. Regulatory requirements pertaining to environmental protection are adhered to without any cost considerations. It was, therefore, theorised that building design teams in developing countries make technical, functional and aesthetic consideration during the infrastructure design stage ahead of environmental considerations.

Originality/value
There is a paucity of studies that have investigated whether building infrastructure designers consider issues of environmental sustainability at the design stage in developing countries. The findings have practical implications on how developing countries can foster environmental sustainability at the design stage and avoid generating a building infrastructure stock that will require environmental resilience adaptation in the future.
Pakistan: a study of market's returns and anomalies
Authors: Tauseef Sana , Dupuy Philippe 
Published: March 2022

Purpose This paper aims to expand foreign investors' understanding of potential return enhancement and risk diversification advantages offered by equity market of Pakistan through comparing its performance to performances in other markets and investigating what matters for investing in Pakistan's market.Design/methodology/approachComparative analysis of Pakistan Stock Exchange is performed using data for 22 developed and 22 emerging markets over the period 1993–2019. Cross-sectional analysis is performed using data for 130 non-financial firms from Pakistan and Carhart (1997) and Fama and French (2015) models are applied. The role of liquidity with five-factor model is analyzed using turnover rate and Amihud (2002) illiquidity cost as liquidity measures.FindingsPakistan's equity offers substantial diversification benefits if added to developed market portfolios. However, observed large returns come together with inverted premia for most traditional factors indicating that investors may want to invest preferably in big stocks with low book-to-market and momentum. Finally, global investors can invest in high yielding stocks with low liquidity risk owing to positive connection between liquidity and returns.Practical implicationsThis study will provide investment model for foreign investors to enhance their portfolio returns. Policy makers in Pakistan must identify regulatory steps to facilitate foreign investments.Originality/valueTo the best of the authors' knowledge, this is the first study which identifies efficiency gains offered by Pakistan's equity for global investors.
How mobile augmented reality digitally transforms the retail sector: examining trust in augmented reality apps and online/offline store patronage intention
Authors: Kang Ju-Young M. , Kim Jae-Eun , Lee Ji Young , Lin Shu Hwa 
Published: March 2022

PurposeThe purpose of the study was to identify (1) whether aspects of expectancy-value judgments (EVJ) of uses and gratifications, such as novelty, fashion/status, sociability and relaxation, influenced trust in augmented reality (AR) apps; (2) whether trust in AR apps influenced usage intention toward AR apps and online/offline store patronage intention and (3) the moderating effect of consumer self-determination.Design/methodology/approachMobile users (n = 630) were drawn from a USA market research company. The proposed model was tested by structural equation modeling with maximum likelihood estimation.FindingsThe study found that trust in AR apps was a determinant of usage intention toward AR apps and online/offline store patronage intention. Novelty and fashion/status for EVJs of uses and gratifications affected trust in AR apps. Sociability for EVJs of uses and gratifications negatively affected trust in AR apps. Users' self-determination moderated the influence of users' trust in AR apps on usage intention toward AR apps and online/offline store patronage intention.Originality/valueFirst, the study elaborates on the impacts of the underlying aspects of an EVJ model of uses and gratifications regarding AR apps on trust in AR apps and EVJ model's influence on usage intention toward AR apps and online/offline store patronage intention. Second, the results of the study suggest useful strategies involved in the development of consumer-driven AR apps that satisfy users' needs and desires.
Behavior of RC flat plate structure subjected to compartment fires
Authors: Abouleiwun Khadejah Alameen , Elbakry Hazem M.F. , Diab Muhammad Ahmed , El-Fitiany S.F. 
Published: March 2022

PurposeIn this study, the behavior of a multi-story flat plate structure during fire exposure is investigated using numerical simulations conducted with using ABAQUS software.Design/methodology/approachA three-dimensional finite element model is then carried out on the RC flat slab structure exposed to standard ISO-834 fire at different location arrangements. The model examines mid-span deflection, shear demand on the columns, bending moment and the membrane action of the floor slab.FindingsThe latter plays a main role to increase the capability and ductility of the slab at longer fire exposure to compensate the reduction in the flexural capacity. Also, shear demand in columns becomes bigger in cases of more than one surrounding slab exposed to fire at the same time.Originality/valueThis work focuses on the influence of the horizontal force on columns due to thermal expansion of slab which should be taken into account in the design of multistory multi-bay building considering it the same as the resulted horizontal force from the wind and seismic effect, the traveling fire and the restraint effect.