bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute caloric restriction acts on the plasma proteome and reveals Apolipoprotein C1 as a signal of nutritional state and metabolic disease
Authors: Vernardis Spyros , Demichev Vadim , Lemke Oliver , Grüning Nana-Maria , Messner Christoph , White Matt , Pietzner Maik , Peluso Alina , Collet Tinh-Hai , Henning Elana , Gille Christoph , Campbell Archie , Hayward Caroline , Porteous David , Marioni Riccardo , Mülleder Michael , Zelezniak Aleksej , Wareham Nick , Langenberg Claudia , Farooqi Sadaf , Ralser Markus 
Published: March 2022

Abstract
Caloric restriction (CR) has proven beneficial to healthspan and lifespan, but not enough is known about the molecular mechanisms that interlink the human CR response and its downstream effectors. We induced acute caloric restriction in a controlled human intervention study and compared plasma proteome, metabolome and endocrine responses. We report that plasma proteins that dominate the response to negative and positive energy balance in CR are associated with disease-relevant endocrine parameters in two population studies. For example, APOC1, a small and understudied apolipoprotein, was not only CR-induced but also dominated the human response to glucose consumption and differed in abundance between individuals with and without diabetes. Our study shows that acute and time-limited CR acts on the plasma proteome, affecting molecular pathways that are intrinsically associated with human endocrine parameters important for metabolic health.
Analysis of porphyromonas gingivalis kgp gene polymorphism in periodontitis patients with diabetes
Authors: Guan Danping , Mao Xiaojun , Jia Ru , Qian Wenhao 
Published: March 2022

Abstract
Background: To analyze the distribution of Porphyromonas gingivalis (P. gingivalis) kgp genotype in subgingival plaque of patients with diabetic periodontitis. The genetic polymorphism of the kgp gene in diabetic periodontitis was explored, and the correlation between different genotypes and diabetic periodontitis was obtained. Methods: Subgingival plaque samples were collected from 49 patients with periodontitis (21 with diabetes, 28 with non-diabetic periodontitis) and 11 healthy periodontal subjects. DNA was extracted, and the positive samples of P. gingivalis were screened by PCR with specific primers of 16S rRNA gene of P. gingivalis, and the fragment of kgp catalytic domain was obtained by PCR with specific primers of kgp. The genotype of kgp was determined by Mse I digestion of the catalytic domain fragment of kgp, and the experimental data were analyzed by the Pearson χ2 method. Results: Among the 11 healthy periodontal population samples collected in this study, 4 cases (36.4 %) of P. gingivalis were detected. Among 49 subgingival plaque samples of periodontitis, 47 patients (95.9 %) were detected with P. gingivalis. There was a statistical difference between the two. Among the 47 samples with P. gingivalis, the detection rate of kgp I was 57.4 %, the detection rate of kgp II was 31.9 %, and the detection rate of kgp I + II was 10.6 %. The kgp genotype was not associated with diabetes. There was no significant difference in the kgp genotypes between the two groups with different PD depths. Conclusion: P. gingivalis is associated with periodontitis. P. gingivalis kgp gene polymorphism exists in patients with diabetic periodontitis subgingival plaque.
Health economic evaluation of digital technologies in nursing care: a review on methodological recommendations
Authors: Huter Kai , Krick Tobias , Rothgang Heinz 
Published: March 2022

Abstract
BackgroundHealth economic evaluation of digital technologies in nursing is important to provide information that help avoid undesirable developments and implementations as well as increasing the chances of success of developed applications. At the same time, studies and evidence on cost-effectiveness are still very rare in this area. Review studies in related technology areas, such as telemedicine, continuously criticize the quality and comparability of the health economic evaluations that have been conducted in this field. Based on a content analysis of methodological literature on the economic evaluation of innovative (digital) technologies in health and nursing this article aims to identify specific challenges in this research area and the given recommendations on how to address these challenges to promote more sound health economic evaluations in the future.MethodsA rapid review was conducted, consisting of a systematic search in the Pubmed database as well as Google Scholar. In addition, the literature lists of the analysed texts were searched for additional texts to be included. Methodological literature, single studies, and reviews were included. A total of 536 studies were screened, of which 29 were included in the full text analysis.ResultsBased on the systematic content analysis of the studies under consideration, 10 special methodological challenges are identified, and it was worked out which methodological recommendations could be considered. A particular focus aimed on whether special methodological approaches might be needed in the context of evaluating the efficiency of digital care technologies. ConclusionMany of the challenges identified for the health economic evaluations of digital nursing technologies are comparable to those of other complex healthcare interventions. The discussed recommendations can help to alleviate those challenges. Future research should focus on alternative approaches to assess causality in different phases of technology development while maintaining high evidence standards. High evidence economic assessment of technologies in nursing care should be carried out in routine use especially if they target to be reimbursed by the social insurance.
Metformin as an adjuvant therapy to an aromatase inhibitor in overweight and obese postmenopausal women with breast cancer: A pilot study
Authors: elattar aya ahmed , Ibrahim Osama Mohamed , Alhassanin Suzan Ahmed , Essa Enas Said , Mostafa Tarek Mohamed 
Published: March 2022

Abstract
Introduction: Metformin may provide therapeutic benefits to patients with various types of malignancy. Through its insulin-sensitizing effect, metformin can also reduce weight in non-diabetic populations.Purpose: Our research aimed at evaluating the effect of metformin as an adjuvant therapy to aromatase inhibitor (letrozole) on estradiol and other biomarkers serum levels in postmenopausal obese breast cancer women.Patients and methods: This controlled study involved 45 postmenopausal breast cancer females who were assigned into three arms: the control arm (arm 1; n= 15 obese women) which received letrozole only (2.5 mg/day); the metformin arm (arm 2; n= 15 obese women) which provided a similar letrozole dosage in addition to metformin (2000 ± 500 mg/day); and the lean arm (arm 3; n= 15) which received letrozole. The intervention duration was 6 months. Blood samples were obtained at zero time and six months later for the measurement of serum estradiol, osteocalcin, insulin, leptin, lactate, and fasting blood glucose. Results: After the intervention, the metformin arm had significantly lower osteocalcin and fasting blood glucose levels than both the control and the lean arms (p<0.0001, p<0.0001, respectively). The metformin and lean arms had significantly lower fasting insulin levels, homeostatic model assessment of insulin resistance (HOMA-IR), estradiol, and leptin concentrations than the control arm (p = 0.0064, p = 0.0020, p<0.0001, and p<0.0001, respectively). We observed non-significant variation in serum lactate levels among the three study arms.Conclusion: Metformin might represent a promising adjuvant therapy to letrozole in postmenopausal women with breast cancer.ClinicalTrials.gov Identifier: NCT05053841/Registered September 23, 2021 - Retrospectively
MiR-106b-5p attenuates neuropathic pain by regulating P2X4 receptor in the spinal cord in mice
Authors: Du Huiying , Wu Danlei , Zhong Shuotao , Wei Xuhong , Yuan Zhongmin , Gong Qingjuan 
Published: March 2022

Abstract
P2X4 receptor (P2X4R) can be up-regulated after nerve injury and its mediated spinal microglial activation makes a critical contribution to pathologically enhanced pain processing in the dorsal horn. Although some studies have partly clarified the mechanism underlying altered P2X4R expression, the specific mechanism is not well understood. MicroRNAs (miRNAs) are small noncoding RNAs, which control gene expression by binding with their target mRNAs. Thus, in the present study, whether miRNA was involved in pathogenesis of neuropathic pain by regulating the P2X4R was investigated. Our results showed that P2X4R was up-regulated in the spinal dorsal horn of mice following spared nerve injury (SNI), and 69 miRNAs (46 up-regulated and 23 down-regulated miRNAs) were differently expressed (fold change > 2.0, P < 0.05). P2X4R was a major target of miR-106b-5p (one of down-regulated miRNAs) with bioinformatics technology and quantitative real time PCR analysis validated the expressed change of miR-106b-5p, dual-luciferase reporter assays confirmed the correlation between them. Fluorescence in situ hybridization was used to show cell co-localization of P2X4R and miR-106b-5p in the spinal dorsal horn. Transfection with miR-106b-5p mimic into BV2 cells reversed the up-regulation of P2X4R induced by LPS. Moreover, miR-106b-5p overexpression significantly attenuated neuropathic pain induced by SNI with decreased expression of P2X4R mRNA and protein in the spinal dorsal horn, intrathecal miR-106b-5p antagomir induced pain behaviors and increased the expression of P2X4R in the spinal dorsal horn of naïve mice. These data suggest that miR-106b-5p can serve as an important regulator of neuropathic pain development by targeting P2X4R.
Trophoblast replacement enables rhesus monkey cloning by somatic cell nuclear transfer
Authors: Sun Qiang 
Published: March 2022

Abstract
Somatic cell nuclear transfer (SCNT) has been used to clone cynomolgus monkeys, but cloning of other non-human primate species remains to be achieved. Our histological examination indicated severe calcification of the placenta of implanted SCNT macaque embryos. By replacing SCNT-derived trophoblast with ICSI-derived trophoblast, endowing a normal placenta for the SCNT fetus, we obtained a healthy SCNT rhesus monkey. Thus, trophoblast replacement represents a useful approach for rhesus monkeys cloning.
Research on the Dynamic Characteristics of the Crack Damage of the Seal-Rotor System
Authors: Huang Fengchao , Wang Lipeng , Luo Yuegang , Chen Changzhen 
Published: March 2022

Abstract
In the rotor system, the labyrinth seal system is the core component to suppress fluid leakage between the rotor and the stator. This paper analyzes the vibration characteristics of the coupling of air-induced vibration and crack fault. First, perform numerical simulation analysis and test verification on the response of the system with no sealing force and crack failure; Subsequently, the system with sealing force and different crack parameters was analyzed for numerical simulation analysis, and then the influence of crack damage failure on other sealing parameters (including sealing pressure difference, sealing gap, and sealing length) was studied; Finally, the influence of double crack damage (damage location, damage degree, phase difference angle) on the rotor system is analyzed. The results show that when the crack depth increases to a certain value, it will also cause the superharmonic resonance phenomenon in the subcritical speed region of the system; When the system has a sealing force, it is found that the airflow excitation frequency of the system can be affected as the degree of crack damage increases; The coupled dynamic response of airflow excitation and crack faults shows a rich spectrum of nonlinear phenomena, which is closely related to the degree of cracks and sealing parameters. Increasing the crack angle will weaken the impact of crack damage on the system. This research can provide a theoretical basis for detecting and diagnosing crack faults in the labyrinth seal rotor system.
A Solitary Hemangioblastoma in the Lateral Ventricle: Case Report and Review of the Literature
Authors: Yu Wenjing , Guo Yifan , Cao Zhijian 
Published: March 2022

Abstract
Background: Hemangioblastoma is a benign tumor occurring in the central nervous system. As known, the most common sites are the cerebellum and spinal cord with the typical appearance which is a nodule with a cyst and contrast-enhanced scan demonstrates the obvious enhancement of the nodule and the local cyst wall. However, it’s difficult to diagnose the hemangioblastoma when it occurs in the lateral ventricle, due to the rare incidence of hemangioblastoma in the lateral ventricle.Case presentation: A 28-year-old man with a 1-month history of recurrent headache accompanied by slight dizziness, then the computed tomography (CT) and magnetic resonance imaging (MRI) of brain showed the cystic solid tumor with obvious enhancement in the parenchymal part and local cystic wall, also, there was abnormal dilated blood vessel in the parenchymal part which was considered as the hemangioblastoma in the lateral ventricle.Conclusions: Total resection of the tumor was performed under neuronavigation, then the tumor was pathologically confirmed to be hemangioblastoma which was the Grade WHOI. Based on this study, we have increased the understanding of hemangioblastoma in the differential diagnosis of tumors located in the lateral ventricle and provide a new method of resection of the tumor.
The Second-Generation Proteasome Inhibitor Ixazomib Enhances the Anticancer Effects of Gemcitabine or Fluorouracil in Pancreatic Cancer Cells
Authors: Gao Jianjun , Wang Hao , Sun Wenjing , Wang Dongsheng , Wang Jiangang , Xie Longfei , Zhong Xiangyu 
Published: March 2022

Abstract
Background
Ixazomib (MLN9708) shows a better pharmacodynamic and pharmacokinetic feature than bortezomib as a second-generation proteasome inhibitor. Our study aims to examine the cytotoxic effects of MLN9708 alone or in combination with fluorouracil (5-FU) or gemcitabine in pancreatic cancer cells, and explore the possible molecular mechanism with MLN9708 in pancreatic cancer.
Materials:
In this preclinical study, three pancreatic cancer cell lines were selected to perform the cell proliferation assays, colony formation assays and anchorage-independent growth assays to examine the cytotoxicity of MLN9708 alone or mixed with gemcitabine or 5-FU. Next-generation RNA sequencing was then used to identify differentially expressed (DE) mRNAs from different treatment groups in BxPC-3 and PANC-1. Quantitative reverse transcription PCR (RT-qPCR) assay was performed to validate the accuracy of the RNA-seq results. Furthermore, UALCAN website and Kaplan-Meier survival analysis were used to determine predicting value of DE expression genes and levels.
Results
MLN9708 demonstrated inhibitory effects on pancreatic cancer cell lines. The combinational therapy leads to greater inhibition of cell proliferation in PANC-1 and BxPC-3 than the monotherapy. A total of 157 commonly DE genes were identified. Among them, top five upregulated DE genes were picked out. RT-qPCR assay showed that ATP6V0D2, Matrix Metalloproteinases-1 (MMP-1) and RNF103-CHMP3 were significant differences. According to UALCAN website and Kaplan-Meier survival analysis, overexpression of ATP6V0D2, MMP-1 and RNF103-CHMP3 was associated with worse prognosis of PC patients; and overexpression of MMP-1 and RNF103-CHMP3 predicted shorter relapse-free survival in pancreatic cancer patients. Furthermore, ATP6V0D2 and MMP-1 demonstrated high expressions in pancreatic cancer samples.
Conclusions
Our experiments suggested MLN9708’s antitumor effects in pancreatic cancer, which sensitizes PANC-1 and BxPC-3 to gemcitabine and 5-FU, especially for 5-FU treatment. MMP-1 overexpression is closely associated with molecular mechanisms of MLN9708 in pancreatic cancer.
Assessing Health Care Workers’ Satisfaction and Loyalty in a Balanced Scorecard: Development and Validation of BSC-HCWSL - a Cross-sectional Study
Authors: Amer Faten , Hammoud Sahar , Khatatbeh Haitham , Alfatafta Huda , Alkaiyat Abdulsalam , Nour Abdulnaser Ibrahim , Boncz Imre , Endrei Dóra 
Published: March 2022

Abstract
BackgroundImproving health care in Palestinian territories is a challenging task. Organizations worldwide utilize the balanced scorecard (BSC) for their performance evaluation (PE). In the BSC implementations, some health care organizations (HCOs) assessed the satisfaction of health care workers (HCWs), but none considered their loyalty. Furthermore, none of them explored the causal links between BSC dimensions and HCWs’ satisfaction based on HCWs’ observations. This research aims to provide a tool to assess the performance of BSC dimensions and their impact on HCWs’ satisfaction and loyalty (BSC-HCWSL). Additionally, it seeks to translate the BSC-HCWSL to Arabic and validate it at Palestinian hospitals.MethodsThis is a cross-sectional study. Two expert committees performed the BSC-HCWSL item development. We performed forward and reverse translations from English to Arabic and vice versa. Pretesting was performed on 13 HCWs at one hospital. Then, between January and October 2021, 540 questionnaires were retrieved in the other 14 hospitals. To detect redundant and poorly correlated items, the composite reliability (CR), interitem correlation (IIC), and corrected item total correlation (CITC) were evaluated. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were used to assess the construct validity.ResultsIn both EFA and CFA, the scales demonstrated a good level of model fit. All the items had loadings greater than 0.50. All constructs passed the discriminant validity. Although certain constructs’ convergent validity was less than 0.50, their CR, IIC, and CITC were adequate. The final best fit model had nine constructs and 28 items in CFA.ConclusionThe BSC-HCWSL is the first self-administered HCW questionnaire designed to assess BSC perspectives and dimensions and their impact on HCWs’ satisfaction and loyalty. The questionnaire scale was completed following all applicable rules and regulations. The findings revealed that this instrument’s psychometric characteristics were adequate. Therefore, BSC-HCWSL can be utilized in future BSC implementations. It can also be used to assess the influence of BSC factors on HCWs’ satisfaction and loyalty and examine differences depending on HCWs and hospital characteristics.
Meta-genomic analysis of Halobacillus trueperi S61 isolated from the Qarhan Salt Lake
Authors: Li Wei , Shen Shuo , Wang Jian 
Published: March 2022

Abstract
Background: Halophilic microbial as prospective resources of biotechnology due to the advantages of flexible survivability. Qarhan Salt Lake is the second largest Salt Lake in the world which contains rich-unique extremophiles and deserved in-depth exploration. Results: Present study first time isolated novel strain Halobacillus trueperi S61 from Qarhan Salt Lake and performed whole-genome sequencing through combined third-generation PacBio and second-generation Illumina technology. The whole genome of Halobacillus trueperi S61 identified 57549 total reads and consists a complete circular chromosome of 4047887 bp with 43.86% GC content without gaps. Total number of 139 non-coding RNA (included 86 tRNA, 30 rRNA and 23 sRNA), 16 gene islands with 260275 bp and two prophages (with 82682 length) were predicted. In addition, the whole genome of Halobacillus trueperi S61 summarized basic annotation for 3982 protein-coding genes, 3980, 3667, 2998 and 2303 unigenes were annotated with Nr, Swissport, KOG and KEGG database. Combined with advanced analysis, 561 carbohydrate enzymes and 4416 pathogen host interactions related genes were identified. The protein function of Halobacillus trueperi S61 was mainly focus on biological processes, and the protein function was mainly distributed in gene transcription and amino acids, and carbohydrates metabolism. Conclusions: The complete whole genome sequence assembly and annotation of novel strain Halobacillus trueperi S61 isolated from Qarhan Salt Lake mainly focus on protein biological processes and antibiotic resistance, provides a potential resource for biotechnology.
Probabilistic Seismic Hazard Analysis and Site Characterization of a Powerplant Site in Chittagong, Bangladesh
Authors: Kolathayar Sreevalsa , Sengupta Soumyadeep 
Published: March 2022

Abstract
In the present study, the Probabilistic Seismic Hazard Analysis (PSHA) has been carried out for a powerplant site in Chittagong using the latest available information on seismicity in the region. The latest updated earthquake catalog homogenized in uniform moment magnitude scale was prepared. Seismicity analysis was carried out, and the seismicity parameters for the region were estimated from the frequency magnitude distribution plot. The peak ground acceleration (PGA) and the spectral acceleration at the bedrock for 10% and 2% probability of exceedance within 50 years were evaluated using different source models and attenuation relations in a logic tree framework. The site was characterized based on the soil investigation data from 31 boreholes and amplification factors considering the local geology (SPT-N values) were obtained through ground response analysis. Subsequently, the surface level peak ground acceleration was estimated and depicted in the form of hazard contour maps.
Amphiphilic porphyrin-based nanoparticles as a nuclei-approaching delivery platform for enhanced cancer photochemotherapy
Authors: Wang Shu-Ping , Huang Xuechun , He Yunhong , Zhou Jun , Tang Guping , Li Shijun , Bai Hongzhen 
Published: March 2022

Abstract
Intelligent porphyrin photosensitizer integrating precise subcellular-targeting capability, enhanced solubility, and efficient phototheranostics is significant for cancer clinical treatment. Herein, we utilize a unilateral amphiphilic porphyrin (P) as nuclei-approaching drug carrier to interact with camptothecin (CPT), a potent DNA topoisomerase I inhibitor, to form supra-amphiphile-assembled nanoparticles (CPT@P). The nanoparticles are capable of directly entering and delivering CPT into nuclei to improve the therapeutic efficacy of photochemotherapy. Cancer cell membrane (M) is further used to camouflage CPT@P, endows the resulting CPT@P«M with excellent stability, homologous tumor targeting ability, and specific capability to co-deliver the chemotherapeutic drug and photosensitizer to tumor cells, even into the nuclei. This work takes advantage of unique structure and intrinsic performance of P to construct an integrative co-delivery system and modify by membrane-coating technology, which not only extends the application scopes of porphyrin photosensitizers but also provides a promising nanoplatform for cancer therapy.