bluuebunny/crossref_metadata_2025_split huggingface dataset:
IGF2BP3 binding circGNB1 promotes the malignant phenotype of GSCs via miR-515-5p/miR-582-3p mediated XPR1 expression change
Authors: Zhang Guoqing , Xu Jinkun , Li Hao , Zhao Junshuang , Hu Jinpeng , Zhao Xiang , Zong Shengliang , Guo Zhengting , Li Xinqiao , Jiang Yang , Jing Zhitao 
Published: June 2022

Abstract
Background:Glioma is the most common and aggressive primary malignant brain tumor. Circular RNAs (circRNAs) and RNA-binding proteins (RBPs) have been verified to mediate diverse biological behaviors in various cancers. Therefore, the aim of this study was to explore novel circRNAs and elucidated relative molecular mechanism in functional phenotypes, which might be a potential prognostic biomarker and therapeutic approach for glioma.Methods:QRT-PCR, western blotting, and immunohistochemistry were used to detect the expression of circGNB1, miR-515-5p, miR-582-3p, XPR1 and IGF2BP3. MTS, transwell, EDU, neurosphere formation and limiting dilution assays were carried out to detect the functional phenotypes of GSCs derived from glioma patients. RIP, RNA pull-down, luciferase reporter, and chromatin immunoprecipitation assays were performed to confirm the underlying molecular mechanisms. Intracranial xenograft was established to determine how circGNB1 impacted in vivo.Results:CircGNB1 was upregulated in glioma and closely associated with the low poor prognosis. Functional assays demonstrated that circGNB1 overexpression promoted glioma cell viability proliferation, invasion, and neurosphere formation. Mechanistically, circGNB1 upregulated the expression of oncogene XPR1 via acting as a sponge for miR-515-5p and miR-582-3p. The following experiments proved XPR1 could promote the malignant phenotype of GSCs via upregulating IL6 expression and activating JAK2/STAT3 signaling. Moreover, the RNA binding protein IGF2BP3 could bind to and maintain the stability of circGNB1, thus promoting the effects of circGNB1 on GSCs.Conclusions:Our study reveals that circGNB1 plays a crucial role in promoting tumorigenesis and malignant progression in glioma, which provides a promising cancer biomarker.
Clinical outcomes of new multifocal intraocular lenses with hydroxyethyl methacrylate and comparative results of contrast sensitivity
Authors: Lee Yongwoo , Choi Chulyoung , Moon Kun , Jeong Yongjin , An Sangil , Lee Jemyung , Lee Jongho , Seong Mincheol 
Published: June 2022

Abstract
Background
We investigate the performance of new hydrophobic diffractive multifocal intraocular lenses (IOL) with hydroxyethyl methacrylate (HEMA) and compare their optical quality, contrast sensitivity, and subjective glare.
Methods
Medical records of patients who underwent routine simple cataract surgery and insertion of an existing multifocal IOL (TFNT, TF group) or a new multifocal IOL (CNWT, CN group) were retrospectively reviewed. Clinical data was collected 2 months postoperatively and included optical quality analysis system (OQAS) indices, contrast sensitivity, and subjective degrees of glare.
Results
135 eyes of 135 patients were included (CN group, 71; TF group, 64). The CN group showed stable visual acuity and a defocus curve. The indices of OQAS did not show a significant difference between groups. Contrast sensitivity was significantly better in the CN group at all degrees, including the area under the log contrast sensitivity function (p = 0.01). The subjective glare survey showed better results for the CN group, with the proportion of patients reporting no glare as 9.9% and 3.1% in the CN and TF groups, respectively. The proportion of patients who reported severe glare was 11.3% in the CN group and 25.0% in the TF group. Although the follow-up period was only 2 months, glistening, surface scattering, and posterior capsule opacity were not observed in any patient.
Conclusions
The new multifocal IOL with HEMA is safe, and provides stable visual acuity as well as superior contrast sensitivity, over the prior IOL.
Effects of high-dose glucose-insulin-potassium on acute coronary syndrome patients undergoing reperfusion therapy: a meta-analysis
Authors: Liu Huiruo , Liu Rugang , Yin Hang , Xu Feng , Chen Yuguo , Li Chuanbao 
Published: June 2022

Abstract
Background: Normal glucose-insulin-potassium (GIK) did not improve clinical outcomes in ACS patients in today’s reperfusion era; however, whether high-dose GIK appeared any cardioprotective function remains uncertain. This meta-analysis aimed to assess the efficacy of high-dose GIK on clinical outcomes in acute coronary syndrome (ACS) patients undergoing reperfusion therapy.Methods: We searched PubMed, Web of science, MEDLINE, Embase and Cochrane Library databases from inception until April 26, 2022 for randomized controlled trials (RCTs) that compared high-dose GIK and placebos in ACS patients receiving reperfusion therapy. The primary endpoint was major adverse cardiovascular events (MACEs), and the secondary endpoints were all-cause mortality, left ventricular ejection fraction (LVEF), heart failure, reinfarction, phlebitis, hyperglycemia, hyperkalemia, hypoglycemia, secondary reperfusion, serum glucose and potassium, activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT).Results: Eleven RCTs with 884 patients were eventually included. Compared with placebos, high-dose GIK markedly reduced MACEs (RR 0.57, 95%CI 0.35 to 0.94, p<0.05) and the risk of heart failure (RR 0.48, 95%CI 0.25 to 0.95, p<0.05), also improved LVEF (MD 2.12, 95%CI 0.32 to 3.92, p<0.05) at 6 months. However, no difference was observed in occurring all-cause mortality at 30 days (RR 0.90, 95%CI 0.35 to 2.28, p=0.82) or 1 year (RR 0.57 95%CI 0.22 to 1.51, p=0.26). Additionally, high-dose GIK was significantly associated with higher occurrence of phlebitis (RR 4.78, 95%CI 1.36 to 16.76, p<0.05), hyperglycemia (RR 9.06, 95%CI 1.74 to 47.29, p<0.01) and hypoglycemia (RR 6.50, 95%CI 1.28 to 33.01, p<0.05), but not reinfarction (RR 0.74, 95%CI 0.36 to 1.50, p =0.40), hyperkalemia (RR 5.99, 95%CI 0.79 to 45.27, p=0.08) or secondary reperfusion (RR 0.91, 95%CI 0.72 to 1.14, p=0.41). As for oxidative stress-lowering function, high-dose GIK markedly reduced SOD activity (SMD -0.77, 95%CI -1.28 to -0.26, p<0.05), but not activity of GSH-Px (SMD 0.34, 95%CI -0.16 to 0.83, p=0.18) or CAT (SMD 0.35, 95%CI -0.14 to 0.84, p=0.16).Conclusions: Patients with ACS receiving reperfusion therapy showed reduction in MACEs and heart failure, improvement of LVEF, as well as good oxidative stress-lowering efficacy in response to high-dose GIK. Simultaneously, higher occurrence of several complications such as phlebitis, hyperglycemia and hypoglycemia accompanied, also a trend to an increase in serum potassium appeared. Furthermore, patients’ all-cause mortality did not modulate any benefits associated with exposure to high-dose GIK. More trails with long-term follow-up are stilled demanded to further verify its efficacy and security to provide powerful evidences for clinical practice.
Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues at the cellular level
Authors: Fan Rong , Zhang Di , Deng Yanxiang , Kukanja Petra , Bartosovic Marek , Su Graham , Bao Shuozhen , Liu Yang , Xiao Yang , Ma Sai , Rosoklija Gorazd , Dowrk Andrew , Mann J. , Leong Kam , Boldrini Maura , Castelo-Branco Goncalo 
Published: June 2022

Abstract
Emerging spatial technologies including spatial transcriptomics and spatial epigenomics are becoming powerful tools for profiling cellular states in the tissue context. However, current methods capture only one layer of omics information at a time precluding the possibility to examine the mechanistic relationship across the cental dogma of molecular biology. Here, we present two spatial multi-omics technologies for spatially resolved genome-wide joint mapping of epigenome and transcriptome by co-profiling chromatin accessibility and gene expression (spatial-ATAC-RNA-seq) or histone modification and gene expression (spatial-CUT&Tag-RNA-seq) on the same tissue section at a resolution near single cells. They were applied to embryonic and neonatal mouse brain as well as adult human brain to map how epigenetic states or modifications regulate cell type and dynamics in tissue. Although distinct tissue features were identified by either spatial epigenome or spatial transcriptome alone with high concordance, we observed their differential roles in defining cell states. In general, epigenetic state proceeds the development of transcriptional phenotype in relation to epigenetic lineage priming. We also observed high expression canonical markers such as PROX1 in the granular cell layer of the human hippocampus showed low chromatin accessibility that corresponded to a low level of RNA turnover rate, highlighting a divergent need for open chromatin or transcription to control cell identity and dynamics. Spatial epigenome-transcriptome co-profiling is a highly informative tool to study the mechanism of gene expression regulation in tissue and may enable a wide range of applications in life science and biomedical research.
Mixed neuroendocrine-non-neuroendocrine neoplasm of the urinary bladder: A case report and review of the literature
Authors: Wang Yuanhao , Li Ning , Zhu Xingwang , Wang Yixiang , Liu﹡ Yili 
Published: June 2022

Abstract
BackgroundThe urinary bladder's mixed neuroendocrine-non-neuroendocrine neoplasm is a sporadic malignant tumor. Because of its rare incidence rate, the optimal treatment options for patients with mixed neuroendocrine-non-neuroendocrine neoplasm of the urinary bladder remain unknown.Case presentationA 61-year-old man was diagnosed with bladder cancer by cystoscopy due to painless gross hematuria. The patient then underwent transurethral resection of the bladder tumor. The postoperative pathological and immunohistochemical examination confirmed a mixed neuroendocrine-non-neuroendocrine neoplasm of the urinary bladder with small cell carcinoma and urothelial. The patient finally received radical cystectomy and cisplatin plus etoposide chemotherapy. There was no recurrence and metastasis three months after the operation.ConclusionThis case is a rare and high-risk pathological type of bladder malignant tumor. After radical cystectomy and postoperative adjuvant treatment, there was no tumor recurrence and metastasis three months after the operation. Radical cystectomy is still the preferred treatment for high-risk bladder cancer without distant metastasis.
Propensity-matched analysis of Ki67 to predict postoperative survival in resectable gastrointestinal stromal tumor patients
Authors: Lu Weidong , Zhang Suxiang , Dong Zhuanxia , Yang Zefeng , Zhao Jian , Wang Jie , Wang Yusheng 
Published: June 2022

Abstract
Objective: To investigate the predictive effect of Ki67 on postoperative overall survive (OS) of patients with resectable gastric stromal tumors (GISTs).Methods: The clinicopathological data and survival status of 281 GIST patients (224 cases of pure gastrointestinal stromal tumor (P-GIST)) and 57 cases of gastrointestinal stromal tumor with other malignant tumors (OMT-GIST)) were retrospectively analyzed; propensity matching analysis and Cox univariate and multivariate analyses were used to verify the predictive effect of Ki67 on OS in patients with resectable GIST.Results: The OS rate differed significantly between P-GIST and OMT-GIST (P<0.001). The ROC curve showed that the best cutoff value of Ki67 was 12.5%. Before and after propensity matching the Ki67 >12.5% and Ki67 ≤12.5% groups, there were significant differences in OS (P=0.002, P=0.003). The univariate analysis showed that tumor diameter, local and distant metastasis, the National Institutes of Health (NIH) , tumor position, and Ki67 were associated with OS (P<0.05). The multivariate analysis showed that Ki67 was an independent prognostic factor in GIST patients (P=0.039). Cutoff value 12.5% of Ki67 could not predict the survival outcome of patients with total GIST (T-GIST) (281 cases) or OMT-GIST (57 cases) (P=0.078, P=0.575).Conclusion: Ki67 can be used to evaluate the postoperative survival outcomes of patients with resectable GIST. The recommended Ki67 cutoff value is 12.5%; however, it is only suitable for P-GIST. OMT-GIST has a poorer prognosis than P-GIST.
Clinicopathology and prognosis of mesonephric carcinomas
Authors: Min Rui , Sun Shuai , Li Fan , Xia Qing , Yan Su , Han Yuexin , Li Nan 
Published: June 2022

Abstract
Background: Mesonephric adenocarcinoma (MNA) and Mesonephric-like adenocarcinoma (MLA) are rare malignant tumors of the female genital tract with extremely similar histopathological, immunohistochemical, and molecular characteristics. Differential diagnosis is therefore challenging, and experience in treatment and prognosis is lacking.Methods: We collected clinicopathological features and immunohistochemical and follow-up information from 2 patients with mesonephric carcinomas (1 case of MNA and 1 case of MLA). A total of 139 cases of MNA and MLA were obtained from PubMed and CNKI through keyword searches. The clinicopathological features and prognostic information of 141 cases of mesonephric carcinomas werecompared and analyzed. This detailed review of the literature provides a comprehensive overview of currently knownmesonephric carcinomas.Results: The median ages of MNA and MLA patients were 51 and 59 years, respectively. The most common clinical symptoms of both patients were irregular vaginal bleeding. Microscopically, these tumor cells are mostly arranged in mixed patterns, such as tubular, glandular and glomeruloid patterns. Tubular and glandular patterns are the most frequently described. MNA is characterized by mesonephric remnants adjacent to tumor tissue. Immunohistochemical staining is positive GATA3, PAX-8 and CD10 expression and negative ER, PR and P16 expression. TTF-1 expression is most common in MLA. The most common genetic change is KRAS gene mutation. The five-year survival rates of MNA and MLA are 73.5% and 68.5%, with recurrence rates of 33% and 58% and median times to recurrence of 20 and 12 months, respectively. Age affects the survival prognosis of MNA and MLA (P=0.04, P<0.01), and there is a significant difference in the DFS K-M survival curve between MNA and MLA (P<0.01).Conclusions: The typical microscopic morphology and expression of immunomarkers support the diagnosis of MNA and MLA. Microscopically, mesonephric remnants adjacent to tumor tissue and immunohistochemical marker TTF-1 expression are key for the differential diagnosis of MNA and MLA. The recurrence and metastasis rates of MLA are much higher than those of MNA, and the prognosis is poor. Age is an important factor influencing the survival and prognosis of MNA and MLA. The most effective treatment for mesonephroid tumors remains to be further elucidated.
Evaluation of the Drying Kinetics of Swirling Fluidized Bed Drying and Performance Assessment on the Nutritional Properties of Bentong Ginger
Authors: Subramaniam Sarmilaah Dewi , Mudalip Siti Kholijah Abdul , Halim Luqman Abdul , Basrawi Firdaus , Azman Nurul Aini Mohd 
Published: June 2022

Abstract
Bentong ginger (BG), grown in a high-altitude area of Bentong, Malaysia, has a similar scientific name to the common ginger species and has been patented by the Malaysian government. BG is known for its larger size and pungent taste and is nutritionally rich in antioxidants such as gingerols, which are responsible for a wide range of pharmacological and physiological effects in human health. This study presents the effect of swirling fluidized bed drying (SFBD) compared with oven drying (OD) and freeze-drying techniques (FD) on the drying kinetics, antioxidant potential, and 6-gingerol concentration of BG. The Midilli-Kucuk model showed the best fit at explaining the thin layer drying behavior of the BG for OD and SFBD, whereas the Page model showed the best fit for FD. The experimental results showed that SFBD reduces the total drying time (250 min) and energy consumption (160.4976 kWh/kg) with higher drying rate (0.1813 g/min) and moisture diffusivity (2.4317 × 10-10 m2/s). In addition, the dried BG sample from the SFBD exhibited a slightly higher DPPH inhibition (89.2%) and the best option to preserve the 6-gingerol compound (2.626 mg/mL) in the Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LC-QTOF/MS) analysis. Thus, the SFBD approach proved to be a feasible method for drying ginger.
Profitability of Agricultural Micro and Small-Scale Enterprise in North Wollo Zone, Amhara Regional State, Ethiopia
Authors: Endris Ebrahim , Kassegn Andualem 
Published: June 2022

Abstract
The contribution of micro and small enterprises is limited as the majority are financially constrained and half of them dropout from business in 2014/15 from Amhara Region. Therefore, this study aimed to analyze the profitability of agricultural micro and small-scale enterprises in North Wollo Zone, Amhara Regional State, Ethiopia. Primary data was collected from 271 sample enterprises, key informants, and focus group discussions. The study employed descriptive and econometrics models for the data analysis. The financial ratio shows that the return on asset, return on owner equity and net profit margin were 0.1601, 0.2768, and 0.1520 birr, respectively. The result of the probit model estimation shows that 6 variables, namely enterprise age, manager education level, credit use, input availability, owners’ aspiration, and frequency of extension contact significantly and positively influenced the probability of MSEs being profitable. The second hurdle model, the truncation model showed that enterprise age, manager education level, record keeping, access to input, and frequency of extension contact significantly affected the extent of agricultural MSEs' profitability. Therefore, enhancing knowledge, skill, and aspirations of enterprise owners, improving financial access and outreach, providing financial bookkeeping training and practice, creating reliable integration with input producers, and frequent extension support to enhance the profitability and sustainability of enterprises.
Paradoxical Effect of Epinephrine on Lesion Redness and Vascularity
Authors: Nazir Zaeem H. , Rishpon Ayelet , Kose Kivanc , Marghoob Nadeem G. , Liopyris Konstantinos , Navarrete-Dechent Cristian , Dusza Stephen W. , Daoud Alexander , Marghoob Ashfaq A. 
Published: June 2022

Abstract
Introduction: Epinephrine is commonly used in combination with local anesthetic (lidocaine/epinephrine) due to its beneficial vasoconstrictive properties. Typically, pallor is appreciated after injection as a sign of effect; however, we observed that some cutaneous malignancies paradoxically revealed increased redness and vascularity after injection of lidocaine/epinephrine. In this study, we investigate this phenomenon among a series of biopsied lesions to identify characteristics of lesions associated with increased redness and/or vascularity.Objectives: To determine characteristics of lesions which become redder or more vascular after injection with lidocaine/epinephrine prior to biopsy. Methods: This cross-sectional study consisted of a convenience sample of lesions scheduled for biopsy. Lesions were photographed prior to and 7 minutes after injection of lidocaine/epinephrine as a part of standard care. Two readers blinded to study objectives and histopathological diagnosis assessed lesions for changes in redness and vascular features.Results: Fifty-four lesions from 47 patients—61.7% male, mean age 64.8 years, age-range 24-91 were included. Thirty-six lesions were biopsy confirmed malignant, with 5 in-situ and 31 invasive malignancies; the remaining 18 lesions were benign. In comparison to non-malignant lesions, malignant lesions were associated with an increase in clinically appreciable vascular features after injection of lidocaine/epinephrine, X2 (1) = 21.600, p<0.001. Further stratification into benign, in-situ, and invasive lesions strengthened the association, X2 (1) = 23.272, p<0.001. Conclusions: Combination lidocaine/epinephrine has been shown to paradoxically increase the visibility of vessels seen in cutaneous malignancies. This is consistent with prior literature suggesting aberrant adrenergic signaling in neoangiogenic vessels.
AGuIX nanoparticles enhance ionizing radiation-induced ferroptosis on tumor cells by targeting the NRF2-GPX4 signaling pathway
Authors: Sun Hao , Cai Hui , Xu Chang , Zhai Hezheng , Lux François , Xie Yi , Feng Li , Du Liqing , Liu Yang , Sun Xiaohui , Wang Qin , Song Huijuan , He Ningning , Zhang Manman , Ji Kaihua , Wang Jinhan , Gu Yeqing , Leduc Géraldine , Doussineau Tristan , Wang Yan , Liu Qiang , Tillement Olivier 
Published: June 2022

Abstract
In the frame of radiotherapy treatment of cancer, radioresistance remains a major issue that still needs solutions to be overcome. To effectively improve the radiosensitivity of tumors and reduce the damage of radiation to neighboring normal tissues, radiosensitizers have been given increasing attention in recent years. As nanoparticles based on the metal element gadolinium, AGuIX nanoparticles have been shown to increase the radiosensitivity of cancers. Although it is a rare nanomaterial that has entered preclinical trials, the unclear biological mechanism hinders its further clinical application. In this study, we demonstrated the effectiveness of AGuIX nanoparticles in the radiosensitization of triple-negative breast cancer. We found that AGuIX nanoparticles increased the level of DNA damage by compromising the homologous recombination repair pathway instead of the non-homologous end joining pathway. Moreover, the results showed that AGuIX nanoparticles induced apoptosis, but the degree of apoptosis ability was very low, which cannot fully explain their strong radiosensitizing effect. Ferroptosis, the other mode of cell death, was also discovered to play a significant role in radiation sensitization, and AGuIX nanoparticles may regulate the anti-ferroptosis system by inhibiting the NRF2-GSH-GPX4 signaling pathway.