bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variscan Inheritance Induces Alpine Upper Crustal Delamination in East Spanish‐Portuguese Central System
Authors: de Vicente G. , Fernández R. Díez , Olaiz A. , Muñoz‐Martín A. 
Published: July 2022

AbstractThe Spanish‐Portuguese Central System (SPCS) is an Alpine Mountain range with crystalline basement characterized by a two‐layer rheological structure. This structure formed after primary (protolith) and secondary (tectonometamorphic) processes during the extensional collapse of the Variscan Orogen. The SPCS structure is usually controlled by foreland‐directed thrusts and strike‐slip faults. However, the eastern SPCS is dominated by NW‐directed, imbricate backthrusts and lacks the main thrust directed to the foreland basin located southeast of the mountain range (Madrid Cenozoic Basin). The SPCS exhibits a crustal root (>40 km depth) supporting SE‐directed crustal‐scale thrusting. Alpine backthrusts sole into an SE‐dipping décollement within the Variscan basement. Variscan extension‐related structures parallel the SE‐dipping geometry of Alpine backthrusts, so they provided favorably oriented rheological weaknesses to accommodate Alpine shortening. Backthrusts geometry, their hanging wall position within the fault that raised the SPCS and gravity modeling support an Alpine crustal delamination process. Tectonic wedging and delamination of the more competent basement occurred in the footwall of Variscan extensional faults (Daurius domain), which enforced the shearing off of a rheologically weaker upper layer of the crust, located in the hanging wall of the Variscan extensional faults (Arriaca domain) by inverting Variscan extensional faults. This led to NW‐directed incipient continental subduction of the weaker crust. Intraplate subduction and crustal delamination can be independent from lithosphere‐scale inheritance and be conditioned by structural inheritance in the overlying crust. Alpine shortening for the Cretaceous cover is around 17.7 km (10.5% shortening), and 11 km (7%) for the upper‐lower crust limit.
The E3 ubiquitin ligase MG53 inhibits hepatocellular carcinoma by targeting RAC1 signaling
Authors: Ma Xiaomin , Ma Xiaoxiao , Zhu Lihui , Zhao Yunxue , Chen Mengmeng , Li Tao , Lin Yueke , Ma Dapeng , Sun Caiyu , Han Lihui 
Published: July 2022

AbstractRas-related C3 botulinum toxin substrate 1 (RAC1) overexpressiosn and hyperactivation are correlated with aggressive growth and other malignant characteristics in a wide variety of cancers including hepatocellular carcinoma (HCC). However, the regulatory mechanism of RAC1 expression and activation in HCC is not fully understood. Here, we demonstrated that E3 ubiquitin ligase MG53 (also known as tripartite motif 72, TRIM72) acted as a direct inhibitor of RAC1, and it catalyzed the ubiquitination of RAC1 and further inhibited RAC1 activity in HCC cells. Mechanistically, MG53 directly bound with RAC1 through its coiled-coil domain and suppressed RAC1 activity by catalyzing the Lys48 (K48)-linked polyubiquitination of RAC1 at Lys5 residue in HCC cells. We further demonstrated that MG53 significantly suppressed the malignant behaviors of HCC cells and enhanced the chemosensitivity of HCC cells to sorafenib treatment by inhibiting RAC1-MAPK signaling axis. In summary, we identified MG53 as a novel RAC1 inhibitor and tumor suppressor in HCC, and it suppressed HCC progression by inducing K48-linked polyubiquitination of RAC1 and further inhibiting the RAC1-MAPK signaling. Altogether, our investigation provided a new therapeutic strategy for RAC1 overactivated tumors by modulating MG53.
RNA-binding protein ZCCHC4 promotes human cancer chemoresistance by disrupting DNA-damage-induced apoptosis
Authors: Zhu Ha , Chen Kun , Chen Yali , Liu Juan , Zhang Xiaomin , Zhou Yumei , Liu Qiuyan , Wang Bingjing , Chen Taoyong , Cao Xuetao 
Published: July 2022

AbstractRNA-binding proteins (RBPs) play important roles in cancer development and treatment. However, the tumor-promoting RBPs and their partners, which may potentially serve as the cancer therapeutic targets, need to be further identified. Here, we report that zinc finger CCHC domain-containing protein 4 (ZCCHC4) is of aberrantly high expression in multiple human cancer tissues and is associated with poor prognosis and chemoresistance in patients of hepatocellular carcinoma (HCC), pancreatic cancer and colon cancer. ZCCHC4 promotes chemoresistance of HCC cells to DNA-damage agent (DDA) both in vitro and in vivo. HCC cell deficiency of ZCCHC4 reduces tumor growth in vivo and intratumoral interference of ZCCHC4 expression obviously enhances the DDA-induced antitumor effect. Mechanistically, ZCCHC4 inhibits DNA-damage-induced apoptosis in HCC cells by interacting with a new long noncoding RNA (lncRNA) AL133467.2 to hamper its pro-apoptotic function. Also, ZCCHC4 blocks the interaction between AL133467.2 and γH2AX upon DDA treatment to inhibit apoptotic signaling and promote chemoresistance to DDAs. Knockout of ZCCHC4 promotes AL133467.2 and γH2AX interaction for enhancing chemosensitivity in HCC cells. Together, our study identifies ZCCHC4 as a new predictor of cancer poor prognosis and a potential target for improving chemotherapy effects, providing mechanistic insights to the roles of RBPs and their partners in cancer progression and chemoresistance.
Maternal immune activation and adolescent alcohol exposure increase alcohol drinking and disrupt cortical-striatal-hippocampal oscillations in adult offspring
Authors: Henricks Angela M. , Sullivan Emily D. K. , Dwiel Lucas L. , Li Judy Y. , Wallin Diana J. , Khokhar Jibran Y. , Doucette Wilder T. 
Published: July 2022

AbstractMaternal immune activation (MIA) is strongly associated with an increased risk of developing mental illness in adulthood, which often co-occurs with alcohol misuse. The current study aimed to begin to determine whether MIA, combined with adolescent alcohol exposure (AE), could be used as a model with which we could study the neurobiological mechanisms behind such co-occurring disorders. Pregnant Sprague-Dawley rats were treated with polyI:C or saline on gestational day 15. Half of the offspring were given continuous access to alcohol during adolescence, leading to four experimental groups: controls, MIA, AE, and Dual (MIA + AE). We then evaluated whether MIA and/or AE alter: (1) alcohol consumption; (2) locomotor behavior; and (3) cortical-striatal-hippocampal local field potentials (LFPs) in adult offspring. Dual rats, particularly females, drank significantly more alcohol in adulthood compared to all other groups. MIA led to reduced locomotor behavior in males only. Using machine learning to build predictive models from LFPs, we were able to differentiate Dual rats from control rats and AE rats in both sexes, and Dual rats from MIA rats in females. These data suggest that Dual “hits” (MIA + AE) increases substance use behavior and disrupts activity in reward-related circuits, and that this may be a valuable heuristic model we can use to study the neurobiological underpinnings of co-occurring disorders. Our future work aims to extend these findings to other addictive substances to enhance the translational relevance of this model, as well as determine whether amelioration of these circuit disruptions can reduce substance use behavior.
N6-methyladenosine modulates long non-coding RNA in the developing mouse heart
Authors: Shen Siman , Liu Keyu , Li Simeng , Rampes Sanketh , Yang Yuhui , Huang Yifeng , Tang Jing , Xia Zhengyuan , Ma Daqing , Zhang Liangqing 
Published: July 2022

AbstractLong non-coding RNAs (lncRNAs) were reported to potentially play a regulatory role in the process of myocardial regeneration in the neonatal mouse. N6-methyladenosine (m6A) modification may play a key role in myocardial regeneration in mice and regulates a variety of biological processes through affecting the stability of lncRNAs. However, the map of m6A modification of lncRNAs in mouse cardiac development still remains unknown. We aimed to investigate the differences in the m6A status of lncRNAs during mouse cardiac development and reveal a potential role of m6A modification modulating lncRNAs in cardiac development and myocardial regeneration during cardiac development in mice. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) of the heart tissue in C57BL/6 J mice at postnatal day 1 (P1), P7 and P28 were performed to produce stagewise cardiac lncRNA m6A-methylomes in a parallel timeframe with the established loss of an intrinsic cardiac regeneration capacity and early postnatal development. There were significant differences in the distribution and abundance of m6A modifications in lncRNAs in the P7 vs P1 mice. In addition, the functional role of m6A in regulating lncRNA levels was established for selected transcripts with METTL3 silencing in neonatal cardiomyocytes in vitro. Based on our MeRIP-qPCR experiment data, both lncGm15328 and lncRNA Zfp597, that were not previously associated with cardiac regeneration, were found to be the most differently methylated at P1-P7. These two lncRNAs sponged several miRNAs which further regulated multiple mRNAs, including some of which have previously been linked with cardiac regeneration ability. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that differential m6A modifications were more enriched in functions and cellular signalling pathways related to cardiomyocyte proliferation. Our data suggested that the m6A modification on lncRNAs may play an important role in the regeneration of myocardium and cardiac development.
Long-range charge carrier mobility in metal halide perovskite thin-films and single crystals via transient photo-conductivity
Authors: Lim Jongchul , Kober-Czerny Manuel , Lin Yen-Hung , Ball James M. , Sakai Nobuya , Duijnstee Elisabeth A. , Hong Min Ji , Labram John G. , Wenger Bernard , Snaith Henry J. 
Published: July 2022

AbstractCharge carrier mobility is a fundamental property of semiconductor materials that governs many electronic device characteristics. For metal halide perovskites, a wide range of charge carrier mobilities have been reported using different techniques. Mobilities are often estimated via transient methods assuming an initial charge carrier population after pulsed photoexcitation and measurement of photoconductivity via non-contact or contact techniques. For nanosecond to millisecond transient methods, early-time recombination and exciton-to-free-carrier ratio hinder accurate determination of free-carrier population after photoexcitation. By considering both effects, we estimate long-range charge carrier mobilities over a wide range of photoexcitation densities via transient photoconductivity measurements. We determine long-range mobilities for FA0.83Cs0.17Pb(I0.9Br0.1)3, (FA0.83MA0.17)0.95Cs0.05Pb(I0.9Br0.1)3 and CH3NH3PbI3-xClx polycrystalline films in the range of 0.3 to 6.7 cm2 V−1 s−1. We demonstrate how our data-processing technique can also reveal more precise mobility estimates from non-contact time-resolved microwave conductivity measurements. Importantly, our results indicate that the processing of polycrystalline films significantly affects their long-range mobility.
Longest sediment flows yet measured show how major rivers connect efficiently to deep sea
Authors: Talling Peter J. , Baker Megan L. , Pope Ed L. , Ruffell Sean C. , Jacinto Ricardo Silva , Heijnen Maarten S. , Hage Sophie , Simmons Stephen M. , Hasenhündl Martin , Heerema Catharina J. , McGhee Claire , Apprioual Ronan , Ferrant Anthony , Cartigny Matthieu J. B. , Parsons Daniel R. , Clare Michael A. , Tshimanga Raphael M. , Trigg Mark A. , Cula Costa A. , Faria Rui , Gaillot Arnaud , Bola Gode , Wallance Dec , Griffiths Allan , Nunny Robert , Urlaub Morelia , Peirce Christine , Burnett Richard , Neasham Jeffrey , Hilton Robert J. 
Published: July 2022

AbstractHere we show how major rivers can efficiently connect to the deep-sea, by analysing the longest runout sediment flows (of any type) yet measured in action on Earth. These seafloor turbidity currents originated from the Congo River-mouth, with one flow travelling >1,130 km whilst accelerating from 5.2 to 8.0 m/s. In one year, these turbidity currents eroded 1,338-2,675 [>535-1,070] Mt of sediment from one submarine canyon, equivalent to 19–37 [>7–15] % of annual suspended sediment flux from present-day rivers. It was known earthquakes trigger canyon-flushing flows. We show river-floods also generate canyon-flushing flows, primed by rapid sediment-accumulation at the river-mouth, and sometimes triggered by spring tides weeks to months post-flood. It is demonstrated that strongly erosional turbidity currents self-accelerate, thereby travelling much further, validating a long-proposed theory. These observations explain highly-efficient organic carbon transfer, and have important implications for hazards to seabed cables, or deep-sea impacts of terrestrial climate change.
Neuropilin 1 and its inhibitory ligand mini-tryptophanyl-tRNA synthetase inversely regulate VE-cadherin turnover and vascular permeability
Authors: Gioelli Noemi , Neilson Lisa J. , Wei Na , Villari Giulia , Chen Wenqian , Kuhle Bernhard , Ehling Manuel , Maione Federica , Willox Sander , Brundu Serena , Avanzato Daniele , Koulouras Grigorios , Mazzone Massimiliano , Giraudo Enrico , Yang Xiang-Lei , Valdembri Donatella , Zanivan Sara , Serini Guido 
Published: July 2022

AbstractThe formation of a functional blood vessel network relies on the ability of endothelial cells (ECs) to dynamically rearrange their adhesive contacts in response to blood flow and guidance cues, such as vascular endothelial growth factor-A (VEGF-A) and class 3 semaphorins (SEMA3s). Neuropilin 1 (NRP1) is essential for blood vessel development, independently of its ligands VEGF-A and SEMA3, through poorly understood mechanisms. Grounding on unbiased proteomic analysis, we report here that NRP1 acts as an endocytic chaperone primarily for adhesion receptors on the surface of unstimulated ECs. NRP1 localizes at adherens junctions (AJs) where, interacting with VE-cadherin, promotes its basal internalization-dependent turnover and favors vascular permeability initiated by histamine in both cultured ECs and mice. We identify a splice variant of tryptophanyl-tRNA synthetase (mini-WARS) as an unconventionally secreted extracellular inhibitory ligand of NRP1 that, by stabilizing it at the AJs, slows down both VE-cadherin turnover and histamine-elicited endothelial leakage. Thus, our work shows a role for NRP1 as a major regulator of AJs plasticity and reveals how mini-WARS acts as a physiological NRP1 inhibitory ligand in the control of VE-cadherin endocytic turnover and vascular permeability.
Ocular counter-roll is less affected in experienced versus novice space crew after long-duration spaceflight
Authors: Schoenmaekers Catho , De Laet Chloë , Kornilova Ludmila , Glukhikh Dmitrii , Moore Steven , MacDougall Hamish , Naumov Ivan , Fransen Erik , Wille Leander , Jillings Steven , Wuyts Floris L. 
Published: July 2022

AbstractOtoliths are the primary gravity sensors of the vestibular system and are responsible for the ocular counter-roll (OCR). This compensatory eye torsion ensures gaze stabilization and is sensitive to a head roll with respect to gravity and the Gravito-Inertial Acceleration vector during, e.g., centrifugation. To measure the effect of prolonged spaceflight on the otoliths, we quantified the OCR induced by off-axis centrifugation in a group of 27 cosmonauts in an upright position before and after their 6-month space mission to the International Space Station. We observed a significant decrease in OCR early postflight, larger for first-time compared to experienced flyers. We also found a significantly larger torsion for the inner eye, the eye closest to the rotation axis. Our results suggest that experienced cosmonauts have acquired the ability to adapt faster after G-transitions. These data provide a scientific basis for sending experienced cosmonauts on challenging missions that include multiple g-level transitions.
Dynamic substrate reactions during room temperature heavy ion irradiation of CoCrCuFeNi high entropy alloy thin films
Authors: Lach Timothy G. , Silva Chinthaka M. , Zhou Yufan , Boldman Walker L. , Rack Philip D. , Weber William J. , Zhang Yanwen 
Published: July 2022

AbstractHigh entropy alloys (HEAs) are promising materials for various applications including nuclear reactor environments. Thus, understanding their behavior under irradiation and exposure to different environments is important. Here, two sets of near-equiatomic CoCrCuFeNi thin films grown on either SiO2/Si or Si substrates were irradiated at room temperature with 11.5 MeV Au ions, providing similar behavior to exposure to inert versus corrosion environments. The film grown on SiO2 had relatively minimal change up to peak damage levels above 500 dpa, while the film grown on Si began intermixing at the substrate–film interface at peak doses of 0.1 dpa before transforming into a multi-silicide film at higher doses, all at room temperature with minimal thermal diffusion. The primary mechanism is radiation-enhanced diffusion via the inverse Kirkendall and solute drag effects. The results highlight how composition and environmental exposure affect the stability of HEAs under radiation and give insights into controlling these behaviors.
Digital quantum simulation of Floquet symmetry-protected topological phases
Authors: Zhang Xu , Jiang Wenjie , Deng Jinfeng , Wang Ke , Chen Jiachen , Zhang Pengfei , Ren Wenhui , Dong Hang , Xu Shibo , Gao Yu , Jin Feitong , Zhu Xuhao , Guo Qiujiang , Li Hekang , Song Chao , Gorshkov Alexey V. , Iadecola Thomas , Liu Fangli , Gong Zhe-Xuan , Wang Zhen , Deng Dong-Ling , Wang H. 
Published: July 2022

AbstractQuantum many-body systems away from equilibrium host a rich variety of exotic phenomena that are forbidden by equilibrium thermodynamics. A prominent example is that of discrete time crystals1–8, in which time-translational symmetry is spontaneously broken in periodically driven systems. Pioneering experiments have observed signatures of time crystalline phases with trapped ions9,10, solid-state spin systems11–15, ultracold atoms16,17 and superconducting qubits18–20. Here we report the observation of a distinct type of non-equilibrium state of matter, Floquet symmetry-protected topological phases, which are implemented through digital quantum simulation with an array of programmable superconducting qubits. We observe robust long-lived temporal correlations and subharmonic temporal response for the edge spins over up to 40 driving cycles using a circuit of depth exceeding 240 and acting on 26 qubits. We demonstrate that the subharmonic response is independent of the initial state, and experimentally map out a phase boundary between the Floquet symmetry-protected topological and thermal phases. Our results establish a versatile digital simulation approach to exploring exotic non-equilibrium phases of matter with current noisy intermediate-scale quantum processors21.
Cryo-EM structure of an active bacterial TIR–STING filament complex
Authors: Morehouse Benjamin R. , Yip Matthew C. J. , Keszei Alexander F. A. , McNamara-Bordewick Nora K. , Shao Sichen , Kranzusch Philip J. 
Published: July 2022

AbstractStimulator of interferon genes (STING) is an antiviral signalling protein that is broadly conserved in both innate immunity in animals and phage defence in prokaryotes1–4. Activation of STING requires its assembly into an oligomeric filament structure through binding of a cyclic dinucleotide4–13, but the molecular basis of STING filament assembly and extension remains unknown. Here we use cryogenic electron microscopy to determine the structure of the active Toll/interleukin-1 receptor (TIR)–STING filament complex from a Sphingobacterium faecium cyclic-oligonucleotide-based antiphage signalling system (CBASS) defence operon. Bacterial TIR–STING filament formation is driven by STING interfaces that become exposed on high-affinity recognition of the cognate cyclic dinucleotide signal c-di-GMP. Repeating dimeric STING units stack laterally head-to-head through surface interfaces, which are also essential for human STING tetramer formation and downstream immune signalling in mammals5. The active bacterial TIR–STING structure reveals further cross-filament contacts that brace the assembly and coordinate packing of the associated TIR NADase effector domains at the base of the filament to drive NAD+ hydrolysis. STING interface and cross-filament contacts are essential for cell growth arrest in vivo and reveal a stepwise mechanism of activation whereby STING filament assembly is required for subsequent effector activation. Our results define the structural basis of STING filament formation in prokaryotic antiviral signalling.
Energy audit and carbon footprint in trawl fisheries
Authors: Sala Antonello , Damalas Dimitrios , Labanchi Lucio , Martinsohn Jann , Moro Fabrizio , Sabatella Rosaria , Notti Emilio 
Published: July 2022

AbstractThe combustion of fossil fuels is considered a major cause of climate change, which is why the reduction of emissions has become a key goal of the Paris climate agreement. Coherent monitoring of the energy profile of fishing vessels through an energy audit can effectively identify sources of inefficiency, allowing for the deployment of well-informed and cost-efficient remedial interventions. We applied energy audits to a test fleet of ten vessels, representing three typical Mediterranean trawl fisheries: midwater pair trawl, bottom otter trawl, and Rapido beam trawl. Overall, these fisheries use approximately 2.9 litres of fuel per kilogram of landed fish, but the fuel consumption rate varies widely according to gear type and vessel size. This amount of fuel burned from capture to landing generates approximately 7.6 kg∙CO2/kg fish on average. Minimising impacts and energy consumption throughout the product chain may be another essential element needed to reduce the environmental costs of fishing. Our results provided a set of recognised benchmarks that can be used for monitoring progress in this field.
Ancient Reef Traits, a database of trait information for reef-building organisms over the Phanerozoic
Authors: Raja Nussaïbah B. , Dimitrijević Danijela , Krause Mihaela Cristina , Kiessling Wolfgang 
Published: July 2022

AbstractTrait-based approaches are increasingly relevant to understand ecological and evolutionary patterns. A comprehensive trait database for extant reef corals is already available and widely used to reveal vulnerabilities to environmental disturbances including climate change. However, the lack of similar trait compilations for extinct reef builders prevents the derivation of generalities from the fossil record and to address similar questions. Here we present the Ancient Reef Traits Database (ARTD), which aims to compile trait information of various reef-building organisms in one single repository. ARTD contains specimen-level data from both published and unpublished resources. In this first version, we release 15 traits for 505 genera and 1129 species, comprising a dataset of 17,841 trait values of Triassic to mid-Holocene scleractinian corals, the dominant reef-builders in the modern ocean. Other trait data, including for other reef-building organisms, are currently being collated.