bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human tumor necrosis factor alpha affects the egg-laying dynamics and glucose metabolism of Schistosoma mansoni adult worms in vitro
Authors: Lopes-Junior Ednilson Hilário , Bertevello Claudio Romero , de Oliveira Silveira Gilbert , Guedes Camila Banca , Rodrigues Gratchela Dutra , Ribeiro Viviane Sousa , Amaral Murilo Sena , Kanamura Cristina Takami , Pinto Pedro Luiz Silva , Krüger Rodrigo Ferreira , Verjovski-Almeida Sergio , Oliveira Katia Cristina 
Published: May 2022

AbstractSeveral studies have described the effects of human tumor necrosis factor alpha (hTNF-α) on Schistosoma mansoni. hTNF-α affects the worm’s development, metabolism, egg-laying, gene expression and protein phosphorylation. The available data on the influence of hTNF-α on egg-laying in S. mansoni are controversial, but understanding the mechanism of egg-laying regulation in this species is essential in combating schistosomiasis. We characterized the effects of in vitro treatment of S. mansoni adult worms with different doses of hTNF-α (5, 20 and 40 ng/ml) for 5 days. We explored the effects on egg-laying rate, glucose levels, ATP metabolism, and messenger RNA (mRNA) expression levels of lactate dehydrogenase, glucose transporters and the parasite gene which acts as an hTNF-α receptor, SmTNFR. hTNF-α influenced egg-laying in a time- and dose-dependent manner: at a dose of 40 ng/ml, egg-laying increased on day 2 and decreased on days 3 and 4; at 20 ng/ml, egg-laying decreased on day 3; while at 5 ng/ml, egg-laying decreased on day 4. The total number of eggs produced was not affected by the different treatments, but the egg-laying dynamics were: the median egg-laying time decreased significantly with treatment, and egg developmental stages and size were also affected. At 5 and 20 ng/ml hTNF-α, lactate production diminished on day 3 up to day 5, while glucose uptake increased on day 5. At 40 ng/ml, glucose uptake diminished on day 1 up to day 3, while ATP accumulation was detected on day 5. No significant changes in mRNA expression were detected in any of the treatments. We found that crosstalk involving hTNF-α and parasite signaling plays a role in the fine-scale regulation of the worm’s metabolism and physiology, and points to new strategies for disease control.
Graphical abstract
Structural comparisons reveal diverse binding modes between nucleosome assembly proteins and histones
Authors: Gill Jasmita , Kumar Anuj , Sharma Amit 
Published: May 2022

AbstractNucleosome assembly proteins (NAPs) are histone chaperones that play a central role in facilitating chromatin assembly/disassembly which is of fundamental importance for DNA replication, gene expression regulation, and progression through the cell cycle. In vitro, NAPs bind to the core histones H2A, H2B, H3, H4 and possibly to H1. The NAP family contains well-characterized and dedicated histone chaperone domain called the NAP domain, and the NAP–histone interactions are key to deciphering chromatin assembly. Our comparative structural analysis of the three three-dimensional structures of NAPs from S. cerevisiae, C. elegans, and A. thaliana in complex with the histone H2A–H2B dimer reveals distinct and diverse binding of NAPs with histones. The three NAPs employ distinct surfaces for recognizing the H2A–H2B dimer and vice versa. Though histones are highly conserved across species they display diverse footprints on NAPs. Our analysis indicates that understanding of NAPs and their interaction with histone H2A–H2B remains sparse. Due to divergent knowledge from the current structures analyzed here, investigations into the dynamic nature of NAP–histone interactions are warranted.
Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities
Authors: Wu Chunyin , Wang Wei , Wang Xiaoqing , Shahid Hamza , Yang Yuting , Wang Yangwen , Wang Shengkun , Shan Tijiang 
Published: May 2022

Abstract
Purpose
Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated.

Methods
The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi.

Results
One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites.

Conclusions
This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries.
Primary retroperitoneal hydatid cyst with intraperitoneal rupture: a case report
Authors: Fourati Kais , Tlili Ahmed , Masmoudi Abderrahmen , Laabidi Taher , Ben Ameur Hazem , Boujelben Salah 
Published: May 2022

Abstract
Background
Hydatid disease is endemic in Mediterranean countries and most commonly occurs in the liver followed by the lung. A primary localization in the retroperitoneum is extremely rare.

Case presentation
We report the case of a 29-year-old Tunisian patient presenting with progressive left flank pain and skin urticaria. On abdominal ultrasonography and computed tomography scan, a ruptured retroperitoneal hydatid cyst was diagnosed, which was confirmed by positive hydatid serology. The treatment consisted of resection of protruding dome. The evolution was favorable. No local recurrence was detected during postoperative follow-up.

Conclusions
Primary retroperitoneal hydatid cyst is extremely rare and has uncommon presentation, but we should learn the keys to its diagnosis. In endemic regions, high suspicion for this disease is justified regardless of localization.
Efficacy and safety of AYUSH-64 as standalone or adjunct to standard care in COVID-19: a structured summary of protocol for a systematic review
Authors: Rai Amit Kumar , Ahmad Azeem , Mundada Pallavi , Kumar V Krishna , Yadav Babita , Khanduri Shruti , Chandrasekhararao Bhogavalli , Srikanth Narayanam 
Published: May 2022

Abstract
Trial registration
Registered with PROSPERO (CRD42021267844) dated 14.07.2021.
Experimental investigation of E-beam effect on the electric field distribution in cross-linked polyethylene/ZnO nanocomposites for medium-voltage cables simulated by COMSOL Multiphysics
Authors: Sharshir A. I. , Fayek S. A. , Abd El-Gawad Amal. F. , Farahat M. A. , Ismail M. I. , Ghobashy Mohamed Mohamady 
Published: May 2022

AbstractThis study investigated the electric field distribution of underground cable insulation in cross-linked polyethylene/zinc oxide (XLPE/ ZnO) nanoparticles (NPs) for medium-voltage (MV) cables. The ZnO NPs that were obtained by three methods of preparation were classified using transmission electron microscopy (TEM). The obtained ZnO NPs were semi-spheres with sizes of 35–55 nm on TEM images. XLPE/ ZnO films with various ZnO NP weight contents (i.e., 0, 1, 3, and 5%) were exposed to varied dosages of 3-MeV electron beam (EB); 0 kGy, 15 kGy, 20 kGy, and 25 kGy. The optimum film XLPE/ 5-ZnO, which has ZnO NP content (5 wt%), irradiated at 25 kGy, according to alternating current (AC)/ DC conductivity (AC: 1 × 10−4 S/m; DC: 12.44 × 10−2 S/m) in minimum relative permittivity (2.24), was obtained. COMSOL Multiphysics was used to simulate the electric field distribution within an MV cable of 25-kGy XLPE/ 5-ZnO insulation. The maximum uniform electric field was found in the middle of the 25-kGy XLPE/5-ZnO film sample, rather than at the top or bottom, which might be attributed to the significantly low relative permittivity of the new 25-kGy XLPE/5-ZnO film cable.
Effect of rinse-off hair conditioner containing argan oil or camellia oil on the recovery of hair damaged by bleaching
Authors: Lee Si Hyun , Ahn Cheunsoon 
Published: May 2022

AbstractEffect of hair conditioner formulated with Argan oil or Camellia oil was investigated on the protection of hair damaged by bleaching. Six different rinse-off type hair conditioners were made with the basic ingredients of hair conditioner and one of the following conditioning agent; Argan oil (AO), Camellia oil (CO), Palmitic acid (PA), Stearic acid (SA), Oleic acid (OA), and Linoleic acid (LA). L*, a*, b* color values and tensile strength, elongation were measured, and the amount of protein leak was examined using the Bradford Protein Assay. Statistical significance was tested using the SPSS statistical software. Although both AO and CO were effective in protecting the tensile properties of bleached hair, significant effects were observed with AO in enhancing the tensile strength and retaining the color of bleached hair. This might be due possibly to the difference in the composition of four major fatty acids in Argan oil and Camellia oil.
Patient-reported outcomes and medical device evaluation: from conception to implementation
Authors: Webber Christina M. , Caldwell Brittany , Bocell Fraser D. , Saha Anindita , Tarver Michelle E. 
Published: May 2022

Abstract
Background
The insights gleaned from patient-reported outcomes (PROs) have implications across the healthcare ecosystem, from clinical investigations to evaluate the safety and effectiveness of medical devices to clinical care and reimbursement decisions. The U.S. Food and Drug Administration’s (FDA) Center for Devices and Radiological Health (CDRH) hosted a public meeting in September 2020 discussing how PROs can be used in medical device evaluation throughout the total product life cycle, as well as methods for developing and modifying PRO instruments to ensure they are fit-for-purpose. This commentary presents key points of discussion from the meeting, providing insight into the increased interest in PRO data to support medical product development while also exploring future opportunities of incorporating PRO data throughout healthcare.

Main Body.
Thoughtful use of fit-for-purpose PRO instruments to integrate the patient’s voice into clinical care paradigms, medical device development, regulatory decisions, and reimbursement and coverage decisions were emphasized throughout the meeting. Existing PRO instruments may be used if the context of use is appropriate. Modifications to an existing PRO instrument may also be explored to ensure the instrument is fit-for-purpose in a new context of use. Development of a novel PRO instrument may be necessary to capture attributes in a new patient population or application. Multi-stakeholder collaborations, of which patients are a key component, create efficiencies in the development and modification of PRO instruments.

Conclusion
Continued multi-stakeholder collaborations bringing together researchers, clinicians, patients, regulators, and payers are critical to further advance the inclusion of the patient voice incorporating PRO instruments throughout the healthcare ecosystem in an efficient manner that is least burdensome to patients.
A combination of two or more unhealthy lifestyle factors is associated with impaired physical and mental health in patients with spondyloarthritis: a cross-sectional study
Authors: Mogard Elisabeth , Bremander Ann , Haglund Emma 
Published: May 2022

Abstract
Background
There is increasing knowledge of how individual lifestyle factors affect patients with spondyloarthritis, while studies exploring the combination of unhealthy lifestyle factors are lacking. Thus, our aim was to study the frequency of two or more unhealthy lifestyle factors and their associations with physical and mental health in patients with spondyloarthritis (SpA).

Methods
A population-based postal survey involving questions on lifestyle factors was completed by 1793 patients with ankylosing spondylitis (AS), psoriatic arthritis (PsA), and undifferentiated spondyloarthritis (USpA). Self-reported physical activity, body mass index, and tobacco use were respectively dichotomized as “healthy” or “unhealthy”, summarized for each patient and stratified into four groups (0–3; 0 = no unhealthy lifestyle factors). Group comparisons were performed with Chi-squared tests, and associations with physical and mental health outcomes were performed with analysis of covariance and logistic regression analysis.

Results
Out of 1426 patients (52% women) with complete information for all studied lifestyle factors, 43% reported ≥ two unhealthy lifestyle factors—more frequently patients with PsA (48%) than AS (39%) or USpA (38%)—and with no difference between women and men (p = 0.399). Two or more unhealthy lifestyle factors were associated with worse health-related quality of life, disease activity, physical function, pain, fatigue, anxiety, and depression, adjusted for age and SpA-subgroup. If an unhealthy level of physical activity was one of the two unhealthy lifestyle factors, patients reported worse health outcomes.

Conclusion
Reporting two or more unhealthy lifestyle factors were associated with worse physical and mental health in patients with SpA. This highlights the need to screen for a combination of unhealthy lifestyle factors and offer individualized coordinated interventions, and tailored coaching to support behavioral change, in order to promote sustainable health.
Prospective Comparative Effectiveness Trial of Multidisciplinary Lung Cancer Care Within a Community-Based Health Care System
Authors: Smeltzer Matthew P. , Ray Meredith A. , Faris Nicholas R. , Meadows-Taylor Meghan B. , Rugless Fedoria , Berryman Courtney , Jackson Bianca , Fehnel Carrie , Pacheco Alicia , McHugh Laura , Robbins Edward T. , Ward Kenneth D. , Klesges Lisa M. , Osarogiagbon Raymond U. 
Published: May 2022

PURPOSE: Multidisciplinary lung cancer care is assumed to improve care delivery by increasing transparency, objectivity, and shared decision making; however, there is a lack of high-level evidence demonstrating its benefits, especially in community-based health care systems. We used implementation and team science principles to establish a colocated multidisciplinary lung cancer clinic in a large community-based health care system and evaluated patient experience and outcomes within and outside this clinic. METHODS: We conducted a prospective frequency-matched comparative effectiveness study (ClinicalTrials.gov identifier: NCT02123797 ) evaluating the thoroughness of lung cancer staging, receipt of stage-appropriate treatment, and survival between patients receiving care in the multidisciplinary clinic and those receiving usual serial care. Target enrollment was 150 patients on the multidisciplinary arm and 300 on the serial care arm. We frequency-matched patients by clinical stage, performance status, insurance type, race, and age. RESULTS: A total of 526 patients were enrolled: 178 on the multidisciplinary arm and 348 on the serial care arm. After adjusting for other factors, multidisciplinary patients had significantly higher odds (odds ratio [OR]: 2.3 [95% CI, 1.5 to 3.4]) of trimodality staging compared with serial care. Patients on the multidisciplinary arm also had higher odds of receiving invasive stage confirmation (OR: 2.0 [95% CI, 1.4 to 3.1]) and mediastinal stage confirmation (OR: 1.9 [95% CI, 1.3 to 2.8]). Additionally, patients receiving multidisciplinary care were significantly more likely to receive stage-appropriate treatment (OR: 1.8 [95% CI, 1.1 to 3.0]). We found no significant difference in overall or progression-free survival between study arms. CONCLUSION: The multidisciplinary clinic delivered significant improvements in evidence-based quality care on multiple levels. Even in the absence of a demonstrable survival benefit, these findings provide a strong rationale for recommending this model of care.
The ultra-low lattice thermal conductivity dominated by the quartic anharmonicity in Bi-based binary compounds A3Bi (A = K, Rb)
Authors: Wang Weiqiang , Wang Xichang , Zhao Yinchang , Meng Sheng , Dai Zhenhong 
Published: May 2022

Abstract
In this article, by using self-consistent phonon (SCP) theory, compressed sensing (CS) technology and Boltzmann transport equation (BTE), we studied the role of quartic anharmonicity in the lattice dynamics and thermal transport characteristics of Bi-based binary compounds A3Bi (A = K, Rb), with a particular focus on unraveling the impacts of quartic anharmonicity on lattice thermal conductivity

. By analyzing the phonon dispersion curve and the phonon density of states, we found that the strong quartic anharmonicity, which is mainly caused by the alkali metal atoms A1 in A3Bi (A = K, Rb), hardens the vibration frequency of the low-lying phonon branch and ensures the lattice dynamic stability. Through the relaxation time approximation of the Boltzmann transport equation, the calculated

considering the three-phonon (3ph) and four-phonon (4ph) scattering process shows an ultra-low value. In addition, we also conducted a detailed analysis of the normalized lattice thermal conductivity, phonon group velocity, phonon scattering rate, and scattering phase space, revealing that the extremely low

in A3Bi is mainly due to its relatively strong 4ph scattering process. Our research proves the existence of strong quartic anharmonicity in A3Bi, and we have studied in detail the influence of 3ph and 4ph scattering processes on the thermal transport properties of A3Bi, which has never been reported before.
Survival Prediction After Neurosurgical Resection of Brain Metastases: A Machine Learning Approach
Authors: Hulsbergen Alexander F. C. , Lo Yu Tung , Awakimjan Ilia , Kavouridis Vasileios K. , Phillips John G. , Smith Timothy R. , Verhoeff Joost J. C. , Yu Kun-Hsing , Broekman Marike L. D. , Arnaout Omar 
Published: May 2022

BACKGROUND:
Current prognostic models for brain metastases (BMs) have been constructed and validated almost entirely with data from patients receiving up-front radiotherapy, leaving uncertainty about surgical patients.

OBJECTIVE:
To build and validate a model predicting 6-month survival after BM resection using different machine learning algorithms.

METHODS:
An institutional database of 1062 patients who underwent resection for BM was split into an 80:20 training and testing set. Seven different machine learning algorithms were trained and assessed for performance; an established prognostic model for patients with BM undergoing radiotherapy, the diagnosis-specific graded prognostic assessment, was also evaluated. Model performance was assessed using area under the curve (AUC) and calibration.

RESULTS:
The logistic regression showed the best performance with an AUC of 0.71 in the hold-out test set, a calibration slope of 0.76, and a calibration intercept of 0.03. The diagnosis-specific graded prognostic assessment had an AUC of 0.66. Patients were stratified into regular-risk, high-risk and very high-risk groups for death at 6 months; these strata strongly predicted both 6-month and longitudinal overall survival (P < .0005). The model was implemented into a web application that can be accessed through http://brainmets.morethanml.com.

CONCLUSION:
We developed and internally validated a prediction model that accurately predicts 6-month survival after neurosurgical resection for BM and allows for meaningful risk stratification. Future efforts should focus on external validation of our model.
midline represses Dpp signaling and target gene expression in Drosophila ventral leg development
Authors: Phillips Lindsay A. , Atienza Markle L. , Ryu Jae-Ryeon , Svendsen Pia C. , Kelemen Lynn K. , Brook William J. 
Published: May 2022

ABSTRACT
Ventral leg patterning in Drosophila is controlled by the expression of the redundant T-box Transcription factors midline (mid) and H15. Here, we show that mid represses the Dpp-activated gene Daughters against decapentaplegic (Dad) through a consensus T-box binding element (TBE) site in the minimal enhancer, Dad13. Mutating the Dad13 DNA sequence results in an increased and broadening of Dad expression. We also demonstrate that the engrailed-homology-1 domain of Mid is critical for regulating the levels of phospho-Mad, a transducer of Dpp-signaling. However, we find that mid does not affect all Dpp-target genes as we demonstrate that brinker (brk) expression is unresponsive to mid. This study further illuminates the interplay between mechanisms involved in determination of cellular fate and the varied roles of mid.
A kinase translocation reporter reveals real-time dynamics of ERK activity in Drosophila
Authors: Yuen Alice C. , Prasad Anadika R. , Fernandes Vilaiwan M. , Amoyel Marc 
Published: May 2022

ABSTRACT
Extracellular signal-regulated kinase (ERK) lies downstream of a core signalling cascade that controls all aspects of development and adult homeostasis. Recent developments have led to new tools to image and manipulate the pathway. However, visualising ERK activity in vivo with high temporal resolution remains a challenge in Drosophila. We adapted a kinase translocation reporter (KTR) for use in Drosophila, which shuttles out of the nucleus when phosphorylated by ERK. We show that ERK-KTR faithfully reports endogenous ERK signalling activity in developing and adult tissues, and that it responds to genetic perturbations upstream of ERK. Using ERK-KTR in time-lapse imaging, we made two novel observations: firstly, sustained hyperactivation of ERK by expression of dominant-active epidermal growth factor receptor raised the overall level but did not alter the kinetics of ERK activity; secondly, the direction of migration of retinal basal glia correlated with their ERK activity levels, suggesting an explanation for the heterogeneity in ERK activity observed in fixed tissue. Our results show that KTR technology can be applied in Drosophila to monitor ERK activity in real-time and suggest that this modular tool can be further adapted to study other kinases.
This article has an associated First Person interview with the first author of the paper.