bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transfer of FRozen Encapsulated multi-donor Stool filtrate for active ulcerative Colitis (FRESCO): study protocol for a prospective, multicenter, double-blind, randomized, controlled trial
Authors: Stallmach Andreas , Grunert Philip , Stallhofer Johannes , Löffler Bettina , Baier Michael , Rödel Jürgen , Kiehntopf Michael , Neugebauer Sophie , Pieper Dietmar H. , Junca Howard , Tannapfel Andrea , Merkel Ute , Schumacher Ulrike , Breternitz-Gruhne Maria , Heller Tabitha , Schauer Anja , Hartmann Michael , Steube Arndt 
Published: February 2022

Abstract
Background
Ulcerative colitis (UC) is a chronic inflammatory bowel disease with significant morbidity and mortality. Although the precise cause remains unknown, disturbances in the intestinal microbial community have been linked to its pathogenesis. Randomized controlled trials in UC and relapsing Clostridioides difficile infection (CDI) have established fecal microbiota (FM) transfer (FMT) as an effective therapy. In this context, preliminary results indicated that the transfer of sterile fecal microbiota filtrates (<0.2 μm; FMF, FMFT) of donor stool also drives gastrointestinal microbiota changes and eliminates symptoms in CDI patients. However, along with the success of FMT, regulatory agencies issued safety alerts following reports of serious adverse events due to transmission of enteric pathogens through FMT. To reduce this risk, we established an extensive test protocol for our donors and quarantine regulations for the produced capsules, but alternative concepts are desirable.

Methods
Our project is a randomized, controlled, longitudinal, prospective, three-arm, multicenter, double-blind study to determine the safety and efficacy of repeated long-term, multi-donor FM or FMF transfers compared to placebo using oral, frozen capsules in 174 randomized patients with mild to moderate active UC. The primary outcome will be clinical remission at week 12.

Discussion
This proposal aims to examine (a) the efficacy of encapsulated transfer of FM and FMF as a therapy for mild to moderate UC, (b) the short- and long-term safety of FMT and FMFT in patients with UC, and (c) the microbial and immunologic changes that occur after FMT and FMFT to help understand how and why it affects inflammatory bowel disease.

Trial registration
ClinicalTrials.govNCT03843385. DRKS (Deutsches Register für Klinische Studien) DRKS00020471
Efficacy of a spot-on combination containing 10% w/v imidacloprid and 1% w/v moxidectin for the treatment of troglostrongylosis in experimentally infected cats
Authors: Traversa Donato , Raue Katharina , Ringeisen Hannah , Blazejak Katrin , Bisterfeld Katrin , Di Cesare Angela , Colombo Mariasole , Böhm Claudia , Strube Christina , Pollmeier Matthias 
Published: February 2022

Abstract
Background
Parasitic bronchopneumonia in domestic cats in Europe, which can manifest with moderate to severe clinical signs, is frequently caused by Troglostrongylus brevior. Data on epizootiological and clinical relevance of cat troglostrongylosis have been published in the last decade but treatment options are still limited. Promising effectiveness data have been generated from clinical cases and field trials for a spot-on formulation containing 1% w/v moxidectin and 10% w/v imidacloprid (Advocate®, Elanco Animal Health). Therefore, two studies have been conducted to confirm under experimental conditions the efficacy of moxidectin 1% contained in Advocate® for the treatment of cat troglostrongylosis.

Methods
Sixteen and 20 cats experimentally infected with T. brevior were included in two separate studies, i.e., Study 1 and 2, respectively. Cats were infected with T. brevior third-stage larvae via gastric tube. In both studies cats were randomized to untreated (control, Group 1) and treatment (Group 2) groups. In Study 1 and Study 2, the two groups comprised eight and 10 cats each. Treated cats received Advocate® spot-on twice at a 4-week interval. The primary efficacy criterion was the number of viable adult T. brevior counted at necropsy. Throughout the trial, the fecal shedding of first-stage larvae (L1) was assessed in treated and untreated control cats.

Results
The experimental model was successful in both studies, as all cats started shedding T. brevior L1 within 25 days post-infection. At necropsy, T. brevior adults were found in 4/8 and 4/10 cats of the control groups in Study 1 and 2, respectively, while none of the treated cats harbored adult worms. The necropsy worm counts in controls did not meet relevant guideline requirements for adequacy of infection, with fewer than six infected cats in the control groups, thus limiting conclusions on treatment efficacy. The fact that 6/8 and 8/10 control cats in Study 1 and 2, respectively, shed L1 up to necropsy while larval shedding ceased in all treated animals after the first treatment provides supporting evidence on the level of efficacy. No remarkable adverse events were recorded in the two studies.

Conclusion
These results indicate that Advocate® spot-on is a safe and effective option for treating cats infected by T. brevior.

Graphical Abstract
A new device-mediated miniprep method
Authors: Mikhail Baryshev , Dmitrijs Merkulov , Ivan Mironov 
Published: February 2022

AbstractSmall-scale plasmid DNA preparation or miniprep is a fundamental technique in estimation cloning experiments and is widely used for DNA methylation analysis in epigenetic research. Current plasmid DNA minipreps use the alkali-SDS-based method in a three-solution format and require spin column-based purification steps. This procedure requires the vortexing or pipetting of pelleted bacteria by centrifugation and manual mixing of the solutions. Here, we describe a centrifuge/mixer-based instrument with the ability to perform centrifugation, vibration, and rotor oscillation in order to perform all steps of plasmid DNA isolation by device only. We found that by applying rotor oscillation-driven mixing of solutions added in the lysis and neutralization steps, homogeneous mixing was achieved within 5 s at a rotor oscillation amplitude of 45° and oscillation frequency of 400 ± 30 rpm, yielding the maximal quantity and quality of plasmid DNA. No increase in host chromosome presence purified by this approach occurs for high-copy-number plasmids compared to manually performed miniprep, and indeed, there is a significant decrease in the presence of the chromosomal fraction in low-copy-number plasmids. The supercoiled form of plasmid DNA purified at a rotor oscillation amplitude of 45° does not turn into an open circular (OC) isoform when the plasmid is stored for 1 year at plus four degrees, in contrast to the plasmid purified with rotor oscillation amplitudes of 270°, 180° and 90°. The programmed time-work-efficient protocol of plasmid miniprep installed in the device gives the extreme simplicity of plasmid minipreps speeding up and facilitating the isolation of plasmid DNAs.
Perioperative fluid administration and complications in emergency gastrointestinal surgery—an observational study
Authors: Voldby Anders W. , Aaen Anne A. , Loprete Roberto , Eskandarani Hassan A. , Boolsen Anders W. , Jønck Simon , Ekeloef Sarah , Burcharth Jakob , Thygesen Lau C. , Møller Ann M. , Brandstrup Birgitte 
Published: February 2022

Abstract
Background
The fluid balance associated with a better outcome following emergency surgery is unknown. The aim of this study was to explore the association of the perioperative fluid balance and postoperative complications during emergency gastrointestinal surgery.

Methods
We retrospectively included patients undergoing emergency surgery for gastrointestinal obstruction or perforation. A perioperative fluid balance of 2.5 L divided the cohort in a conservative and liberal group. Outcome was Clavien-Dindo graded complications registered 90 days postoperatively. We used logistic regression adjusted for age, sex, American Society of Anesthesiologists’ classification, use of epidural analgesia, use of vasopressor, type of surgery, intraabdominal pathology, and hospital. Predicted risk of complications was demonstrated on a continuous scale of the fluid balance.

Results
We included 342 patients operated between July 2014 and July 2015 from three centers. The perioperative fluid balance was 1.6 L IQR [1.0 to 2.0] in the conservative vs. 3.6 L IQR [3.0 to 5.3] in the liberal group. Odds ratio of overall 2.6 (95% CI 1.5 to 4.4), p < 0.001, and cardiopulmonary complications 3.2 (95% CI 1.9 to 5.7), p < 0.001, were increased in the liberal group. A perioperative fluid balance of 0–2 L was associated with minimal risk of cardiopulmonary complications compared to 1.5–3.5 L for renal complications.

Conclusion
We found a perioperative fluid balance above 2.5 L to be associated with an increased risk of overall and cardiopulmonary complications following emergency surgery for gastrointestinal obstruction or perforation. A perioperative fluid balance of 0–2 L was associated with the lowest risk of cardiopulmonary complications and 1.5–3.5 L for renal complications.
Explaining offenders’ longitudinal product-specific target selection through changes in disposability, availability, and value: an open-source intelligence web-scraping approach
Authors: Quinn Liam , Clare Joseph , Lindley Jade , Morgan Frank 
Published: February 2022

Abstract
Objective
To address the gap in the literature and using a novel open-source intelligence web-scraping approach, this paper investigates the longitudinal relationships between availability, value, and disposability, and stealing counts of specific makes and models of gaming consoles.

Methods
Using data from Western Australia (2012–2019) and focusing on specific makes/models of gaming consoles, the relationships between product-specific stealing counts, availability, value, and disposability were examined using time series and cross-sectional analyses.

Results
Support was found for a positive relationship between the changing disposability of specific makes/models of gaming consoles over their lifecycle with corresponding stealing counts, above and beyond changes in availability and value. However, when these attributes were analysed statically, both disposability and value were important.

Conclusions
The results highlight the importance of measuring correlates of ‘hot products’ longitudinally to better understand offenders’ target selection preferences over time—with important implications for theft risk assessment and crime prevention policy and practice. These findings also provide support for the use of similar open-source intelligence web-scraping strategies as a suitable technique for capturing time-specific proxies for product-specific value and disposability.
Combined inhibition of HMGCoA reductase and mitochondrial complex I induces tumor regression of BRAF inhibitor-resistant melanomas
Authors: de Groot Evelyn , Varghese Sruthy , Tan Lin , Knighton Barbara , Sobieski Mary , Nguyen Nghi , Park Yong Sung , Powell Reid , Lorenzi Philip L. , Zheng Bin , Stephan Clifford , Gopal Y. N. Vashisht 
Published: February 2022

AbstractBackgroundPrimary and posttreatment resistance to BRAFV600mutation–targeting inhibitors leads to disease relapse in a majority of melanoma patients. In many instances, this resistance is promoted by upregulation of mitochondrial oxidative phosphorylation (OxPhos) in melanoma cells. We recently showed that a novel electron transport chain (ETC) complex I inhibitor, IACS-010759 (IACS), abolished OxPhos and significantly inhibited tumor growth of high-OxPhos, BRAF inhibitor (BRAFi)–resistant human melanomas. However, the inhibition was not uniform across different high OxPhos melanomas, and combination with BRAFi did not improve efficacy.MethodsWe performed a high-throughput unbiased combinatorial drug screen of clinically relevant small molecules to identify the most potent combination agent with IACS for inhibiting the growth of high-OxPhos, BRAFi-resistant melanomas. We performed bioenergetics and carbon-13 metabolite tracing to delineate the metabolic basis of sensitization of melanomas to the combination treatment. We performed xenograft tumor growth studies and Reverse-Phase Protein Array (RPPA)–based functional proteomics analysis of tumors from mice fed with regular or high-fat diet to evaluatein vivomolecular basis of sensitization to the combination treatment.ResultsA combinatorial drug screen and subsequent validation studies identified Atorvastatin (STN), a hydroxymethylglutaryl-coenzyme A reductase inhibitor (HMGCRi), as the most potent treatment combination with IACS to inhibitin vitrocell growth and induce tumor regression or stasis of some BRAFi-resistant melanomas. Bioenergetics analysis revealed a dependence on fatty acid metabolism in melanomas that responded to the combination treatment. RPPA analysis and carbon-13 tracing analysis in these melanoma cells showed that IACS treatment decreased metabolic fuel utilization for fatty acid metabolism, but increased substrate availability for activation of the mevalonate pathway by HMGCR, creating a dependence on this pathway. Functional proteomic analysis showed that IACS treatment inhibited MAPK but activated AKT pathway. Combination treatment with STN counteracted AKT activation.ConclusionsSTN and other clinically approved HMGCRi could be promising combinatorial agents for improving the efficacy of ETC inhibitors like IACS in BRAFi-resistant melanomas.
Assessing incarcerated women’s physical and mental health status and needs in a Swiss prison: a cross-sectional study
Authors: Augsburger Aurélie , Neri Céline , Bodenmann Patrick , Gravier Bruno , Jaquier Véronique , Clair Carole 
Published: February 2022

Abstract
Background
Women make up 5% of the European prison population on average. Almost invisible in prison and health research, and suffering the stigma associated with female offending, incarcerated women are often forgotten, and their specific healthcare needs remain much ignored. Combining face-to-face survey interviews and medical chart data, we aim to assess the health status, healthcare needs, and access to preventive medicine of women incarcerated in Switzerland.

Results
Sixty incarcerated adult women participated in a cross-sectional study to assess their life and incarceration histories, physical and mental health problems, medication, and use of medical services. Eligibility criteria were (a) an incarceration of at least four weeks and (b) the ability to provide written informed consent. Exclusion criteria were psychiatric instability and insufficient language competence.
Women’s average age was 34.3 years old (SD = 9.8); 45.0% of them were born in Switzerland, 33.3% in Europe and 15.0% on the African continent. Overall, 61.7% of women self-reported physical or mental health problems and 13.3% indicated they were once diagnosed with a sexually transmitted infection. Further, 78.3% of women were active cigarette smokers; more than one in three women reported alcohol use problems and almost one in two women had used at least one illicit drug in the year before incarceration. Depression and perceived stress scores were above clinical cut-off points for more than half of interviewed women. When asked how they rated their health, 68.3% of women felt it had worsened since incarceration. All but four women had accessed prison medical services; however, our study does not indicate whether women’s use of healthcare was indeed adequate to their needs.

Conclusions
This study demonstrated incarcerated women’s poor health and health-risk behaviours. Structural changes and gender-responsive health promotion interventions have the potential to improve the health of incarcerated women and help them return to the community in better health.
Generalization of fear of movement-related pain and avoidance behavior as predictors of work resumption after back surgery: a study protocol for a prospective study (WABS)
Authors: Masuy Rini , Bamelis Lotte , Bogaerts Katleen , Depreitere Bart , De Smedt Kris , Ceuppens Jeroen , Lenaert Bert , Lonneville Sarah , Peuskens Dieter , Van Lerbeirghe Johan , Van Schaeybroeck Patrick , Vorlat Peter , Zijlstra Steefka , Meulders Ann , Vlaeyen Johan W. S. 
Published: February 2022

Abstract
Background
Previous studies indicated that about 20% of the individuals undergoing back surgery are unable to return to work 3 months to 1 year after surgery. The specific factors that predict individual trajectories in postoperative pain, recovery, and work resumption are largely unknown. The aim of this study is to identify modifiable predictors of work resumption after back surgery.

Methods
In this multisite, prospective, longitudinal study, 300 individuals with radicular pain undergoing a lumbar decompression will be followed until 1-year post-surgery. Prior to surgery, participants will perform a computer task to assess fear of movement-related pain, avoidance behavior, and their generalization to novel situations. Before and immediately after surgery, participants will additionally complete questionnaires to assess fear of movement-related pain, avoidance behavior, optimism, expectancies towards recovery and work resumption, and the duration and severity of the pain. Six weeks, 3 months, 6 months, and 12 months after surgery, they will again complete questionnaires to assess sustainable work resumption, pain severity, disability, and quality of life. The primary hypothesis is that (generalization of) fear of movement-related pain and avoidance behavior will negatively affect sustainable work resumption after back surgery. Second, we hypothesize that (generalization of) fear of movement-related pain and avoidance behavior, negative expectancies towards recovery and work resumption, longer pain duration, and more severe pain before the surgery will negatively affect work resumption, pain severity, disability, and quality of life after back surgery. In contrast, optimism and positive expectancies towards recovery and work resumption are expected to predict more favorable work resumption, better quality of life, and lower levels of pain severity and disability after back surgery.

Discussion
With the results of this research, we hope to contribute to the development of strategies for early identification of risk factors and appropriate guidance and interventions before and after back surgery.
Trial registration The study was preregistered on ClinicalTrials.gov: NCT04747860 on February 9, 2021.
Factors Influencing Team Performance: What Can Support Teams in High-Performance Sport Learn from Other Industries? A Systematic Scoping Review
Authors: Salcinovic Benjamin , Drew Michael , Dijkstra Paul , Waddington Gordon , Serpell Benjamin G. 
Published: February 2022

AbstractBackgroundThe primary aim of our systematic scoping review was to explore the factors influencing team function and performance across various industries and discuss findings in the context of the high-performance sport support team setting. These outcomes may also be used to inform future research into high-performance teamwork in sport.MethodsA systematic scoping review of literature published in English since 2000 reporting team-based performance outcomes and included a performance metric that was ‘team outcome based’ was conducted using search of the Academic Search Ultimate, Medline, Business Source Ultimate, APA PsycInfo, CINAHL, SPORTDiscus, and Military database (ProQuest) using the terms: ‘team’, ‘function’ OR ‘dysfunction’, ‘Perform*’ OR ‘outcome’.ResultsApplication of the search strategy identified a total of 11,735 articles for title and abstract review. Seventy-three articles were selected for full-text assessment with the aim to extract data for either quantitative or qualitative analysis. Forty-six of the 73 articles met our inclusion criteria; 27 articles were excluded as they did not report a performance metric. Eleven studies explored leadership roles and styles on team performance, three studies associated performance feedback to team performance, and 12 studies explored the relationship between supportive behaviour and performance. Team orientation and adaptability as key figures of team performance outcomes were explored in 20 studies.ConclusionsOur findings identified 4 key variables that were associated with team function and performance across a variety of industries; (i) leadership styles, (ii) supportive team behaviour, (iii) communication, and (iv) performance feedback. High-performance teams wishing to improve performance should examine these factors within their team and its environment. It is widely acknowledged that the dynamics of team function is important for outcomes in high-performance sport, yet there is little evidence to provide guidance. This inequality between real-world need and the available evidence should be addressed in future research.
Antagonistic and growth-promoting potential of multifarious bacterial endophytes against Fusarium wilt of chickpea
Authors: Khanna Annie , Raj Kushal , Kumar Pankaj , Wati Leela 
Published: February 2022

AbstractBackgroundChickpea is the most important legume crop in India, and its productivity is significantly lowered due to biotic constraints such as wilt caused byFusarium oxysporumf. sp. ciceris. Endophytes help plants to thrive better under stress conditions by regulating plant hormones and inducing systemic resistance. The aim of the present study was to identify chickpea root bacterial endophytes having antagonistic ability for the management of theFusariumwilt.ResultsTwenty endophytic bacterial strains were isolated from the susceptible and resistant chickpea cultivar, amongst which 35% bacterial endophytes gave positive results for siderophore and 15% isolates were HCN producers, whereas 55% showed good growth on ACC-supplemented medium. Based upon 16S rRNA phylogenetic analysis, efficient endophytic bacterial isolates CRBE1, CRBE3 and CRBE7 were identified asPriestia megaterium, Brucella haematophilaandMicrobacterium paraoxydans, respectively. Bacterial endophyte CRBE7 and CRBE3 showed pronounced antagonistic activity againstFusarium oxysporumf. sp.cicerisunder in vitro conditions. Inoculation of chickpea with isolated bacterial endophytes alone and in combination through seed priming resulted in significant biocontrol activity againstFusariumwilt of chickpea under screenhouse conditions. Biopriming of chickpea seeds alone with CRBE3 and as consortium of CRBE3 + CRBE7 upsurged the phenol content in susceptible and resistant chickpea varieties JG 62 and HC 3, which indicated the role of promising endophytes as potential bio-agents under in vivo conditions.ConclusionsChickpea root bacterial endophytesPriestia megaterium(CRBE1),Brucella haematophila(CRBE3) andMicrobacterium paraoxydans(CRBE7) exhibiting antagonistic activity could be incorporated in integrated disease management module against Fusarium wilt of chickpea.