bluuebunny/crossref_metadata_2025_split huggingface dataset:
The complete genome sequence of elite bread wheat cultivar, “Sonmez”
Authors: Akpinar Bala Ani , Leroy Philippe , Watson-Haigh Nathan , Baumann Ute , Barbe Valerie , Budak Hikmet 
Published: June 2022

High-yielding crop varieties will become critical in meeting the future food demand in the face of worsening weather extremes and threatening biotic stressors. The bread wheat cultivar Sonmez-2001 is a registered variety that is notable for its performance under low-irrigation conditions, which further improves upon irrigation. Additionally, Sonmez-2001 is resilient against certain biotic stressors, particularly soil-borne pathogens. Here, we provide a reference-guided whole genome sequence of Sonmez-2001, assembled into 21 chromosomes of the A, B and D genomes and totaling 13.3 gigabase-pairs in length. Additionally, a de novo assembly of an additional 1.05 gigabase-pairs was generated that represents either Sonmez-specific sequences or sequences that considerably diverged between Sonmez and Chinese Spring. Within this de novo assembly, we identified 35 gene models, of which 11 were high-confidence, that may contribute to the favorable traits of this high-performing variety. We identified up to 24 million sequence variants, of which up to 2.4% reside in coding sequences, that can be used to develop molecular markers that should be of immediate use to the cereal community.
Case Report: Takotsubo cardiomyopathy in a postoperative patient without cardiological disease
Authors: Coaguila-Cusicanqui Luis , Castillo-Atoche Vanessa , Montalvo-Suyon Roberto , Cavero-Reyes Yuriko , Failoc-Rojas Virgilio E. 
Published: June 2022

Background: Takotsubo cardiomyopathy (TC) is characterized by a clinical presentation that mimics acute coronary syndrome but is reversible. Alterations of Takotsubo in patients without previous heart disease remain a challenge for diagnosis. Case report: We present a case of an 80-year-old patient from Peru. The patient underwent surgery, with the diagnosis of Chilaiditi’s syndrome. One day after surgery, she presented with dyspnea, tachycardia, and electrocardiographic changes. The diagnosis of Takotsubo syndrome with cardiogenic shock and renal failure on hemodialysis was made. She was hospitalized in the Intensive Care Unit and was managed with vasopressors and nitroglycerin. There was no cardiac lesion in the cineangiogram or occlusion of arteries. The patient was extubated and received daily dialysis until discharge. Conclusions: Takotsubo is an emotional, non-cardiac, or post-traumatic stressful event that triggers myocardial injury with segmental anomalous, the possible etiology of which is the release of an endothelial neurotransmitter caused by stress. Emergency physicians should be aware of this as even patients without previous cardiac pathologies when exposed to stressors (such as surgeries) develop emergency symptomatology similar to myocardial infarction. Thus, emergency physicians should identify any cardiac abnormalities after a stressor, as well as be prepared for the diagnosis of TC.
Farmers’ knowledge, perceptions, and practices on animal trypanosomosis and the tsetse fly vector: A cross-sectional study around Kenya’s Arabuko-Sokoke Forest Reserve at the livestock-wildlife interface
Authors: Serem Erick K , Bargul Joel L , Ngari Moses M , Abdullahi Osman A , Mburu David M 
Published: June 2022

Background: Animal African trypanosomosis (AAT) is a veterinary disease caused by trypanosomes transmitted cyclically by tsetse flies. AAT causes huge agricultural losses in sub-Saharan Africa. Both tsetse flies and trypanosomosis (T&T) are endemic in the study area inhabited by smallholder livestock farmers at the livestock-wildlife interface around Arabuko-Sokoke Forest Reserve (ASFR) in Kilifi County on the Kenyan coast. We assessed farmers’ knowledge, perceptions and control practices towards T&T. Methods: A cross-sectional study was conducted during November and December 2017 to collect data from 404 randomly selected cattle-rearing households using a structured questionnaire. Descriptive statistics were used to determine farmers’ knowledge, perceptions, and control practices towards T&T. Demographic factors associated with knowledge of T&T were assessed using a logistic regression model. Results: Participants consisted of 53% female, 77% married, 30% elderly (>55 years), and the majority (81%) had attained primary education or below. Most small-scale farmers (98%) knew the tsetse fly by its local name, and 76% could describe the morphology of the adult tsetse fly by size in comparison to the housefly’s (Musca domestica). Only 16% of the farmers knew tsetse flies as vectors of livestock diseases. Higher chances of adequate knowledge on T&T were associated with the participants’ (i) age of 15–24 years (aOR 2.88 (95% CI 1.10–7.52), (ii) level of education including secondary (aOR 2.46 (95% CI 1.43–4.24)) and tertiary (aOR 3.80 (95% CI 1.54–9.37)), and (iii) employment status: self-employed farmers (aOR 6.54 (95% CI 4.36–9.80)). Conclusions: Our findings suggest that small-scale farmers around ASFR have limited knowledge of T&T. It is envisaged that efforts geared towards training of the farmers would bridge this knowledge gap and sharpen the perceptions and disease control tactics to contribute to the prevention and control of T&T.
Cost-utility analysis of cast compared to removable brace in the management of adult patients with ankle fractures
Authors: Nwankwo Henry , Mason James , Costa Matthew L. , Parsons Nicholas , Redmond Anthony , Parsons Helen , Haque Aminul , Kearney Rebecca S. 
Published: June 2022

Aims To compare the cost-utility of removable brace compared with cast in the management of adult patients with ankle fracture. Methods A within-trial economic evaluation conducted from the UK NHS and personnel social services (PSS) perspective. Health resources and quality-of-life data were collected as part of the Ankle Injury Rehabilitation (AIR) multicentre, randomized controlled trial over a 12-month period using trial case report forms and patient-completed questionnaires. Cost-utility analysis was estimated in terms of the incremental cost per quality adjusted life year (QALY) gained. Estimate uncertainty was explored by bootstrapping, visualized on the incremental cost-effectiveness ratio plane. Net monetary benefit and probability of cost-effectiveness were evaluated at a range of willingness-to-pay thresholds and visualized graphically. Results The incremental cost and QALYs of using brace over a 12-month period were £46.73 (95% confidence interval (CI) £-9 to £147) and 0.0141 (95% CI -0.005 to 0.033), respectively. The cost per QALY gained was £3,318. The probability of brace being cost-effective at a £30,000 per QALY willingness-to-pay threshold was 88%. The results remained robust to a range of sensitivity analyses. Conclusion This within-trial economic evaluation found that it is probable that using a removable brace provides good value to the NHS when compared to cast, in the management of adults with ankle fracture. Cite this article: Bone Jt Open 2022;3(6):455–462.
One Health and surveillance of zoonotic tuberculosis in selected low-income, middle-income and high-income countries: A systematic review
Authors: de Macedo Couto Rodrigo , Santana Giulia Osório , Ranzani Otavio T. , Waldman Eliseu Alves 
Published: June 2022

Background
Little is known about zoonotic tuberculosis (zTB) due to Mycobacterium bovis burden across the globe. The aim of this study was to describe zTB surveillance programs in selected WHO signatory countries and to assess the relationship of the disease with the country’s income level and the risk of M. bovis transmission.

Methods
We searched the main articles databases and grey literature for guide documents published between 1980 and 2019. For inclusion, the articles and guide documents had to be in English, French, Portuguese, Spanish, or Italian. Only original articles and narrative and systematic reviews were accepted and the guide documents were required to be available on official websites. We excluded articles that did not focus on epidemiology, control and surveillance. We used bovine TB cases in livestock and wildlife populations as a proxy for the country’s risk of zTB using data from the World Organization for Animal Health (OIE) published from 2015 to 2018. Countries were classified according to income level (World Bank’s classification) and strength of zTB surveillance. The study was registered in PROSPERO under number CRD42018090603.

Findings
We included 13 articles and 208 guide documents including data from 119/194 countries (61.3%). We found a lack of surveillance data about zTB in over half (89.9%) of the 119 WHO signatory countries. Most surveillance systems perform passive surveillance and are not integrated into the One Health perspective, which was operating in 4/119 (3.4%) countries, all high-income. Many of these countries (71/119, 59.7%) have M. bovis circulating in their cattle herds, but only ~10% of them have implemented zTB surveillance activities.

Interpretation
Our findings highlight weaknesses in zTB surveillance worldwide, with a consequent lack of information that could support an adequate understanding of disease burden, especially in countries at major risk for M. bovis transmission. To meet this challenge, efforts will be needed to promote intersectoral policies, implementing the One Health strategy.
A comparison of risk factors for cryptosporidiosis and non-cryptosporidiosis diarrhoea: A case-case-control study in Ethiopian children
Authors: Johansen Øystein Haarklau , Abdissa Alemseged , Zangenberg Mike , Mekonnen Zeleke , Eshetu Beza , Sharew Bizuwarek , Moyo Sabrina , Sommerfelt Halvor , Langeland Nina , Robertson Lucy J. , Hanevik Kurt 
Published: June 2022

Background
Cryptosporidiosis is a major cause of diarrhoea in young children in low-and-middle-income countries. New interventions should be informed by evidence pertaining to risk factors and their relative importance. Inconsistencies in the literature may to some extent be explained by choice of methodology, furthermore, most previous risk factor studies compared cryptosporidiosis cases to diarrhoea cases of other aetiologies rather than with controls without diarrhoea.

Methodology/Principal findings
We investigated a broad set of factors in under-2-year-olds presenting with diarrhoea to a hospital and a health center in southwestern Ethiopia. We applied quantitative cut-offs to distinguish between cryptosporidiosis and incidental Cryptosporidium infection or carriage, a hierarchical causal framework to minimize confounding and overadjustment, and a case-case-control design, to describe risk factors for both cryptosporidiosis and non-cryptosporidiosis diarrhoea. Moderate and severe acute malnutrition were strongly associated with both cryptosporidiosis and non-cryptosporidiosis diarrhoea. Previous healthcare attendance and low maternal education were only associated with cryptosporidiosis, whereas unsafe child stool disposal, prematurity and early cessation of exclusive breastfeeding were significantly associated with non-cryptosporidiosis diarrhoea only. By estimation of population attributable fractions, socioeconomic factors—specifically low maternal education—and public tap water use, were apparently more important risk factors for cryptosporidiosis than for non-cryptosporidiosis diarrhoea.

Conclusions/Significance
Nutritional management of moderate acute malnutrition may be an effective intervention against cryptosporidiosis, particularly if combined with targeted therapy for cryptosporidiosis which, again, may mitigate nutritional insult. Focused caregiver education in healthcare settings and follow-up of children with acute malnutrition may prevent or improve outcomes of future episodes of cryptosporidiosis.
Noncommunicable diseases risk factors and the risk of COVID-19 among university employees in Indonesia
Authors: Widyahening Indah Suci , Vidiawati Dhanasari , Pakasi Trevino A. , Soewondo Pradana , Ahsan Abdillah 
Published: June 2022

Introduction
Noncommunicable diseases (NCDs) are still a major public health problem in Indonesia. Studies have shown that risk factors of NCDs are associated with coronavirus disease 2019 (COVID-19) severity and mortality. However, it is unclear whether NCD risk factors are also risks for new COVID-19 cases. This study aimed to obtain an NCD risk profile among university employees and its associations with contracting COVID-19.

Methods
A cross-sectional study was conducted in October 2021. Participants were administrative employees of Universitas Indonesia (UI), Depok City, West Java. Assessment of NCD risk factors was based on the World Health Organization STEPwise approach to NCD risk factor surveillance (WHO STEPS). Demographic, working, and medical-history data were obtained electronically by using a Google Form. Physical and laboratory examinations were done in the Integrated Post for NCDs. Risks were expressed as adjusted odds ratio (ORadj) and 95% confidence interval (CI) in multivariate analyses.

Results
A total of 613 employees were enrolled. Men were predominant (54.8%), and about 36% of them work in shift as security personnel. About 66.7% were overweight or obese and 77.8% had hypertension. There were 138 (22.8%) employees who had COVID-19. Nearly all (95.6%) had been fully vaccinated against COVID-19. At-risk waist circumference (ORadj 1.72, 95% CI 1.15–2.56, p = 0.008) and total cholesterol level of 200–239 mg/dL (ORadj 2.30, 95% CI 1.19–4.44, p = 0.013) were independent risk factors, but shift work (ORadj 0.52, 95% CI 0.34–0.80, p = 0.003) was protective against COVID-19.

Conclusion
The prevalence of NCD risk factors among university administrative employees was high, increasing the risk of contracting COVID-19. A behavioral intervention program to manage NCD risk factors at the university level is urgently needed according to the Health Promoting University framework.
Risk factors for disease severity and increased medical resource utilization in respiratory syncytial virus (+) hospitalized children: A descriptive study conducted in four Belgian hospitals
Authors: Proesmans Marijke , Rector Annabel , Keyaerts Els , Vandendijck Yannick , Vermeulen Francois , Sauer Kate , Reynders Marijke , Verschelde Ann , Laffut Wim , Garmyn Kristien , Fleischhackl Roman , Bollekens Jacques , Ispas Gabriela 
Published: June 2022

Background
We aimed to provide regional data on clinical symptoms, medical resource utilization (MRU), and risk factors for increased MRU in hospitalized respiratory syncytial virus (RSV)-infected Belgian pediatric population.

Methods
This prospective, multicenter study enrolled RSV (+) hospitalized children (aged ≤5y) during the 2013–2015 RSV seasons. RSV was diagnosed within 24h of hospitalization. Disease severity of RSV (+) patients was assessed until discharge or up to maximum six days using a Physical Examination Score (PES) and a derived score based on ability to feed, dyspnea and respiratory effort (PES3). MRU (concomitant medications, length of hospitalization [LOH], and oxygen supplementation) was evaluated. Kaplan-Meier survival analysis was performed to compare MRU by age and presence of risk factors for severe disease. Association between baseline covariates and MRU was analyzed using Cox regression models.

Results
In total, 75 children were included, Median (range) age was 4 (0–41) months, risk factors were present in 18.7%, and early hospitalization (≤3 days of symptom onset) was observed in 57.3% of patients. Cough (100%), feeding problems (82.2%), nasal discharge (87.8%), and rales and rhonchi (82.2%) were frequently observed. Median (range) LOH and oxygen supplementation was 5 (2–7) and 3 (1–7) days. Oxygen supplementation, bronchodilators, and antibiotics were administered to 58.7%, 64.0%, and 41.3% of the patients, respectively. Age <3 months and baseline total PES3 score were associated with probability and the duration of receiving oxygen supplementation. LOH was not associated with any covariate.

Conclusion
RSV is associated with high disease burden and MRU in hospitalized children. Oxygen supplementation but not length of hospitalization was associated with very young age and the PES3 score. These results warrant further assessment of the PES3 score as a predictor for the probability of receiving and length of oxygen supplementation in RSV hospitalized children.

Registration
NCT02133092
The association between vitamin D status and COVID-19 in England: A cohort study using UK Biobank
Authors: Lin Liang-Yu , Mulick Amy , Mathur Rohini , Smeeth Liam , Warren-Gash Charlotte , Langan Sinéad M. 
Published: June 2022

Background
Recent studies indicate that vitamin D supplementation may decrease respiratory tract infections, but the association between vitamin D and COVID-19 is still unclear.

Objective
To explore the association between vitamin D status and infections, hospitalisation, and mortality due to COVID-19.

Methods
We used UK Biobank, a nationwide cohort of 500,000 individuals aged between 40 and 69 years at recruitment between 2006 and 2010. We included people with at least one serum vitamin D test, living in England with linked primary care and inpatient records. The primary exposure was serum vitamin D status measured at recruitment, defined as deficiency at <25 nmol/L, insufficiency at 25–49 nmol/L and sufficiency at ≥ 50 nmol/L. Secondary exposures were self-reported or prescribed vitamin D supplements. The primary outcome was laboratory-confirmed or clinically diagnosed SARS-CoV-2 infections. The secondary outcomes included hospitalisation and mortality due to COVID-19. We used multivariable Cox regression models stratified by summertime months and non-summertime months, adjusting for demographic factors and underlying comorbidities.

Results
We included 307,512 participants (54.9% female, 55.9% over 70 years old) in our analysis. During summertime months, weak evidence existed that the vitamin D deficiency group had a lower hazard of being diagnosed with COVID-19 (hazard ratio [HR] = 0.86, 95% confidence interval [CI] = 0.77–0.95). During non-summertime, the vitamin D deficiency group had a higher hazard of COVID-19 compared with the vitamin D sufficient group (HR = 1.14, 95% CI = 1.01–1.30). No evidence was found that vitamin D deficiency or insufficiency was associated with either hospitalisation or mortality due to COVID-19 in any time strata.

Conclusion
We found no evidence of an association between historical vitamin D status and hospitalisation or mortality due to COVID-19, along with inconsistent results for any association between vitamin D and diagnosis of COVID-19. However, studies using more recent vitamin D measurements and systematic COVID-19 testing are needed.
Aprendizaje Servicio, Género y TIC. De la desigualdad de género en las TIC a la generación de vocaciones tecnológicas en el ámbito educativo
Authors: Vergés Bosch Núria , Freude Leon , Camps Calvet Clara , Collado Sevilla Ana Atonia 
Published: June 2022

Pese a los esfuerzos de las últimas décadas, las desigualdades de género en el acceso a los estudios y trabajos TIC persisten. Aunque la exclusión de las mujeres de las TIC responde a múltiples causas, la investigación coincide en la necesidad de despertar el interés y generar más vocaciones tecnológicas entre las chicas desde edades escolares y, con ello, incrementar el número de estudiantes y trabajadoras TIC en el futuro. Para dar respuesta a esta necesidad diseñamos e implementamos un proyecto de Aprendizaje Servicio (ApS) en el ámbito de la Sociología del Género en Barcelona. El proyecto consistía en llevar a cabo conferencias-taller en torno al género y la tecnología para las escuelas y trabajar cuestiones de desigualdad de género, socialización de género y visibilización de las mujeres en las TIC, así como, vías para acceder a ellas. En este artículo nos proponemos exponer la experiencia de 4 cursos consecutivos y sus principales resultados. Para su descripción y evaluación aplicamos una metodología mixta, cuantitativa y cualitativa. Contamos, por un lado, con los informes de autoreflexión de nuestro alumnado universitario participante y por el otro, con las encuestas a 284 escolares y a sus profesores. Aunque con algunas limitaciones, la experiencia ha sido muy satisfactoria, tanto para el profesorado como para los alumnados participantes. Los resultados muestran que el ApS en perspectiva de género tiene un gran potencial para el aprendizaje, así como, para la transformación social y de género.
A little doubt saves many mistakes: Early and late error detection in copy-typing
Authors: Dahm Stephan F. , Rieger Martina 
Published: June 2022

Abstract
Based on internal predictions, action-errors can be detected relatively early. Different kinds of sensory feedback further provide information about the occurrence of errors later on. To investigate the mechanisms underlying error detection in copy-typing, ten-finger-typists and hunt-and-peck-typists copy typed with and without visibility of the screen and keyboard. We expected that error detection evolves in slower typing before, during, and after an error. Results showed that more errors were reported with visible screen than with covered screen in both groups underpinning the importance of distal action-effects for error detection. Importantly, ten-finger-typists showed pre-error-slowing in the inter-keystroke-intervals (IKIs) before reported errors, but hunt-and-peck-typists did not. In both groups, error-slowing was observed in the last IKI before both reported and unreported errors. Hence, internal predictions play a role in error detection in both groups, but in ten-finger-typists, internal models may be more precise, leading to earlier error detection. Alternatively, slowing down may increase the probability of detecting errors. Finally, in both groups post-error-slowing indicates that sensory feedback from performing keystrokes contributes to error detection. In conclusion, feedback from distal action-effects (i.e., the screen), movement related feedback, and predictive mechanisms contribute to error detection in typing.
Thalamocortical Projections Are Significantly Impaired in the R6/2 Mouse Model of Huntington’s Disease
Authors: Holley S. M. , Oikonomou K. D. , Swift C. M. , Mohan L. , Matthews B. , Vega O. , Mkrtchyan G. , Cepeda C. , Levine M. S. 
Published: June 2022

AbstractAs Huntington’s disease (HD) progresses, there is a significant loss of neurons in the striatum in addition to a distinct thinning of the cerebral cortex. Despite an early presence of sensorimotor deficits in patients with HD, electrophysiological studies designed to assess the integrity of thalamocortical circuits are sparse. Using the R6/2 mouse model of HD, we provide evidence of reduced connectivity between thalamic cells and their targeted cortical regions. Whole-cell patch clamp recordings from ventral anterolateral nucleus (VAL; motor) and ventral posteromedial nucleus (VPM; somatosensory) thalamic neurons inex vivobrain slices of R6/2 and wild-type (WT) mice revealed that cells in both thalamic nuclei of R6/2 mice exhibited significant differences in passive and active cell membrane properties (smaller cell membrane capacitances, faster decay time constants and increased input resistances) compared with WT cells. Although only cells in the VPM of symptomatic R6/2 mice had more depolarized resting membrane potentials compared with WTs, cells in both nuclei displayed increased excitability in symptomatic, but not presymptomatic, R6/2 mice. Optical activation of VAL and VPM terminals elicited smaller magnitude current responses in cortical pyramidal neurons (CPNs) in both motor cortex (M1CTX) and somatosensory barrel cortex (BCTX) of symptomatic R6/2 mice compared with CPNs in WT mice. Furthermore, we observed a decrease in the frequency of thalamocortical excitatory quantal events in R6/2 BCTX CPNs, with no genotype-dependent differences in AMPA:NMDA response amplitude ratios. These data suggest there is a decrease in the transmission of thalamocortical information that is likely because of impaired neurotransmitter release.