bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of genetic diamine oxidase (DAO) deficiency in women with fibromyalgia in Spain
Authors: Okutan Gülşah , Casares Eva Ruiz , Alcalde Teresa Perucho , Niño Guerthy Melissa Sánchez , Penadés Bruno F. , Lora Ana Terrén , Martin Ismael San Mauro 
Published: December 2022

Abstract
Diamine oxidase (DAO) is the enzyme responsible for the metabolism of intestinal histamine. Single nucleotide polymorphisms (SNPs) of the AOC1 gene are associated with low enzymatic activity or functionality in the metabolism of histamine.
The objectives of the present study were to determine the prevalence of DAO deficiency for four variants of the AOC1 gene, p.Thr16Met (rs10156191), p.Ser332Phe (rs1049742), p.His664Asp (rs1049793) and c.691G>T (rs2052129) in Spanish women with fibromyalgia, as well as to compare the distribution of allelic and genotypic frequencies with European population samples in Hardy-Weinberg equilibrium (HWE) extracted from the ALFA (Allele Frequency Aggregator) database.
The sample consisted of 98 Spanish women with fibromyalgia between 33 and 60 years old (48.5 years ± 7.5) DAO enzyme activity was determined by a sample of oral mucosa and a standard hygiene protocol was followed. The patients' DNA was extracted and the analysis of gene variants of interest was performed using SNPE Multiplex (Single Nucleotide Primer Extension).
The prevalence of genetic DAO deficiency was 74.5% by the four variants of the AOC1 gene. The deficit for each SNP followed the following frequencies: p.Thr16Met (53.1%), c.691G>T (49%), p.His664Asp (48%) and p.Ser332Phe (19.4%). The allelic and genotypic prevalence of the variants had similar distributions of European population except for p.Ser332Phe.
Variants of the AOC1gene could be associated with genetic DAO deficiency and potential disruptive biomarker in fibromyalgia patients.
This study was registered in ClinicalTrials.gov Identifier: NCT05389761
Optimization analysis of fire characteristics and emergency evacuation scheme of small- and medium-sized high-speed railway stations
Authors: Chunhua Zhang , Jianing Jin , Dengming Jiao , Liqiang Wang 
Published: December 2022

Abstract
To study the fire spread law in high-speed railway stations and suggest a reasonable evacuation plan, we first establish a physical model based on a high-speed railway station in the Shandong building using Pyrosim software. We then simulate and analyze the variation rules for the smoke temperature, CO volume fraction, and visibility in the commercial mezzanine and waiting-hall space for a left-side fire and identify the most critical factors affecting personnel evacuation. Anylogic software, which considers the crowd social forces as well as the effect of visibility on personnel speed in areas where the fire is dynamically spreading, is used to compare and analyze the free-evacuation scheme and different guided evacuation schemes in terms of the required safety evacuation time. The optimal fire emergency evacuation scheme is then proposed. The results show that the smoke from a fire on the left commercial mezzanine will seriously threaten the upper stair entrance of the commercial mezzanine. Notably, visibility is the most critical factor affecting personnel evacuation. Under the free evacuation scheme, the utilization of evacuation exits is not balanced, and unsurprisingly, personnel congestion at the station entrance occurs. This affects evacuation efficiency. Accordingly, changing the evacuation personnel path and evacuation exit based on the shortest path under equal-area zoning evacuation can effectively reduce the personnel evacuation time.
Antimicrobial use and resistance in Scottish dairy herds: A survey of farmers’ knowledge, behaviours and attitudes
Authors: Borelli Elena , Ellis Kathryn , Tomlinson Martin , Hotchkiss Emily 
Published: December 2022

Abstract
Understanding how antimicrobials are used on dairy farms, as well as stakeholder beliefs relating to their use, is essential to ensure responsible antimicrobial usage (AMU) and tackle antimicrobial resistance (AMR). An online questionnaire was designed based on the findings of two focus groups. It was structured into four sections covering AMU and AMR knowledge, behaviour, and attitudes of farmers. The target population was all Scottish dairy farmers (n = 832). The survey was completed by 61 respondents. Overall, participants expressed a lack of knowledge of antimicrobials and AMR. Farmers highlighted the importance of veterinarian support, as vet opinions and advice were ranked significantly more important than other social referents or advisors. The vast majority of respondents (90%) had reduced AMU on farms over recent years. However, some risky practices, such as feeding antibiotic milk to calves, are still widespread. Limited finances and knowledge were the main barriers to ensuring responsible AMU. Attitudes towards AMR were variable, and some farmers expressed a mismatch between their intention and behaviour. Results indicated that AMU had been reduced, and most farms implemented best practices. Yet, more work is needed to raise AMR awareness among farmers and address the main barriers.
Rebuilding Caribbean Environmental Health Post-Crisis Programs: Case Study for Virtual Mentorship
Authors: DeVito Rosann , Landeen Elizabeth , Dyjack David , Labbo Rebecca , Gill Gagandeep , Gerding Justin , Kalis Martin , Daly Scott , Lopez Raymond , Somaiya Chintan , Chera Sukhdeep , Vanover Christine , Randhawa Manjit 
Published: December 2022

Abstract
After the U.S. Caribbean 2017 hurricanes, rebuilding, strengthening, and sustaining essential environmental health (EH) services and systems were critical. The National Environmental Health Association, in partnership with the U.S. Centers for Disease Control and Prevention (CDC), developed an online mentorship program for both newly hired and existing environmental health staff, as well as health department leadership in the Caribbean health departments. Participants were provided both practical and didactic learning and were given the opportunity to evaluate the program. Both mentors and mentees were highly satisfied with the knowledge and skills acquired, and mentees expressed it was relevant to their daily work. Based on the findings, we recommend both an online and a hybrid mentorship program be considered for leadership- and inspector-level workforces in EH and potentially other fields.
SL-PseAAC: A super learner based DNA-binding protein prediction model
Authors: AHMED SHEIKH HASIB , BOSE DIBYENDU BRINTO , KHANDOKER RAFI , RAHMAN M. SAIFUR 
Published: December 2022

Abstract
Background: DNA-binding proteins (DNA-BPs) are the proteins that bind and interact with DNA. DNA-BPs play a critical role in all aspects of genetic activity. DNA-BPs regulate and affect the processes of transcription and DNA replication, repair, and recombination by organizing the chromosomal DNA as well as various cellular processes. Very few proteins, however, are DNA-binding in nature. Therefore, it is necessary to develop an efficient predictor for identifying DNA-BPs.
Result: In this work, we have proposed new benchmark datasets for the DNA-binding protein prediction problem. We discovered several quality concerns with the widely used benchmark datasets, PDB1075 (for training) and PDB186 (for independent testing), which necessitated the preparation of new benchmark datasets. Our proposed datasets UNIPROT1424 and UNIPROT356 can be used for model training and independent testing respectively. We have retrained selected state-of-the-art DNA-BP predictors in the new datasets and reported their performance results. We also trained a novel predictor using the new benchmark datasets. We extracted features from various feature categories, then used a Random Forest classifier and Recursive Feature Elimination with Cross-validation (RFECV) to select the optimal set of 452 features. We then proposed a super learner architecture as our final prediction model. Named Super Learner Using Pseudo Amino Acid Composition, or SL-PseAAC in short, our model achieved 91.86%, 92.14% and 92.70% accuracy in 10-fold cross-validation, jackknife and independent testing respectively.
Conclusion: SL-PseAAC has performed very well in cross-validation testing and has outperformed all the state-of-the-art prediction models in independent testing. Its performance scores in cross-validation testing generalized very well in the independent test set. The source code of the model is publicly available at https://github.com/HasibAhmed1624/SL-PseAAC. Therefore, we expect this generalized model can be adopted by researchers and practitioners to identify novel DNA-binding proteins.
Active illumination spectacle device slows childhood myopia progression
Authors: Kubota Ryo , O'Connor Brett , Samandarova Inna , Selenow Arkady , Ali Steven , Mitchell G Lynn 
Published: December 2022

Abstract
Childhood myopia progression occurs due to excessive axial elongation of the eye and results in increased risk for sight-threatening ocular comorbidities. Increased daily time spent outdoors and sustained use of peripheral defocus modifying lenses have been shown to exert anti-myopigenic effects in children. Here, we evaluate a novel spectacle device which projected bright, broad spectrum light combined with myopic peripheral retinal defocus in children. It was theorized that a significant decrease in myopic cycloplegic spherical equivalent autorefraction (CSER) and axial elongation would be observed in treated eyes of subjects who used the device 1.5 hours per day, five days per week, versus age- and ethnicity-matched untreated myopic virtual controls. Projected one-year myopic CSER increase and axial elongation were found to be significantly less in treated eyes, with estimated treatment effects of 0.46 D for CSER and -0.20 mm for axial length versus virtual controls. These treatment effects after part-time wear are similar to values reported for other spectacle and contact lens therapies which are worn on a full time basis for myopia control, which may suggest the novel study device exerts myopic inhibition effects via unique or multifactorial pathways.
Factors associated with father-infant bonding in the COVID-19 pandemic: An internet-based cross-sectional study in Japan
Authors: Nishimura Etsuko , Shoki Rina , Kato Mika , Yoneoka Daisuke , Okawa Sumiyo , Tabuchi Takahiro , Ota Erika 
Published: December 2022

Abstract
The Covid-19 pandemic has forced changes in our lifestyles and affected the relationships between father and infant. The aim of this study was to explore factors associated with father-infant bonding during the Covid-19 pandemic in Japan. This study was a cross-sectional study using a nationwide online survey data. The Japanese version of the Mother-to-Infant Bonding Scale (MIBS) was used for father-infant bonding. The study participants were divided into two groups depending on their partners’ parity. A linear regression model (Gauss-Markov-type) was used for the two groups. A total of 1,055 men were included in the analysis. Of these men, 521 (49.4%) had a partner who was primipara, and 534 (50.6%) had a partner who was multipara. No significant differences were found between the two groups for MIBS-J scores. The fathers’ mental health, relationship with the partner and family members, abusive behavior towards children, wanted pregnancy, and admission history to NICU for the youngest child were associated with father-infant bonding. As for factors related to Covid-19, caring for the child while the partner is at home has a negative impact on bonding, while fear related to infection with Covid-19 has no negative impact on the bonding.
Different effects of the chemically similar foodborne flavonoids genistein, genistin, and daidzein on the inhibition of proliferation and induction of apoptosis in U251 glioma cells
Authors: Li Meng , Huang Qiming , Xie Liuming , Qian Zilan , Yang Haiyun , Shi Xiaoyi , Huang Zhibing 
Published: December 2022

Abstract
Glioma is one of the most aggressive diseases among malignant tumors. Chemotherapy has a very important role in the treatment of glioma. However, most western medicines elicit different adverse reactions in patients along with obvious side effects. Therefore, there is a clinical need to develop new antitumor drugs with low toxicity and good therapeutic effects to reduce the mortality of cancer patients. The different effects of foodborne flavonoids genistein (GST), genistin (GIN), and daidzein (DAI) on glioma U251 cells have not been studied. Therefore, this study explored the effects of these flavonoids on U251 cells. CCK-8 assays, fluorescence microscopy, and flow cytometry were used to detect the different effects of flavonoids on the proliferation, morphology, and apoptosis of glioma U251 cells, respectively. Reverse transcription-PCR and western blotting were carried out to detect the expression of caspase-3, Bax, PI3K, mTOR, and AKT at mRNA and protein levels, respectively. U251 cell viability was reduced with treatment at different concentrations of GST, GIN, and DAI. GST and GIN promoted apoptosis by upregulating the expression of Bax and caspase-3, whereas DAI promoted apoptosis by downregulating p-AKT and p-mTOR. This study provides a theoretical basis for the use of flavonoids in the treatment of gliomas.
Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities
Authors: Ma Lay-Sun , Lin Yu-Han , Xu Meng-Yun , Hsu Chuan-Chih , Lee Hui-Chun , Tsai Wei-Lun , Damei Florensia , Hoang Cuong , Chiang Yin-Ru 
Published: December 2022

Abstract
Diversification of effector function, driven by a co-evolutionary arms race, enables pathogens to establish compatible interactions with their hosts. Structurally conserved plant pathogenesis-related PR-1 and PR-1-like (PR-1L) proteins are involved in plant defense and fungal virulence, respectively. It is unclear how fungal PR-1L counteracts plant defense. Here, we show that Ustilago maydis UmPR-1La and yeast ScPRY1 with conserved phenolic detoxification functions are Ser/Thr-rich region-mediated cell-surface localization proteins. However, UmPR-1La has gained additional specialized activity in eliciting hyphal-like formation, suggesting that U. maydis deploys UmPR-1La to sense phenolics and direct their growth in plants. U. maydis also hijacks plant cathepsin B-like 3 (CatB3) to release functional CAPE-like peptides after cleaving a conserved CNYD motif of UmPR-1La to subvert plant immunity for promoting fungal virulence. Surprisingly, CatB3 avoids cleavage of plant PR-1s, despite the presence of the same conserved CNYD motif. Our work highlights that UmPR-1La has acquired additional dual roles to suppress plant defense and sustain the infection process of fungal pathogens.
Radiofrequency ablation of atrioventricular nodal reentrant tachycardia with speech induced left atrial tachycardia: a case report
Authors: Wei Jingxun , Xu Huipu 
Published: December 2022

Abstract
BACKGROUND: Repetitive atrial tachycardias account for 10–15% of arrhythmias in adults undergoing electrophysiology examination for paroxysmal supraventricular tachycardia. Speech-induced atrial tachycardia is rarely reported worldwide, and atrioventricular nodal reentrant tachycardia combined with speech-induced left atrial tachycardia is rarer.
CASE PRESENTATION: A 65-year-old man who was diagnosed with atrioventricular nodal reentrant tachycardia at admission, and atrial tachycardia occurred after radiofrequency ablation. Simultaneously, his speech was confirmed as the cause of almost persistent left atrial tachycardia. The earliest potential was mapped at the right lower pulmonary vein orifice using the CARTO system. After ablation, atrial tachycardia was terminated but the trigger mechanism remained unclear and may be related to the left atrial cardiac plexus.
CONCLUSION: Although the physiological basis of speech-induced atrial tachyarrhythmia is currently speculative, it may be due to the combination of increased vagal stimulation. Radiofrequency ablation is an effective treatment method, which can definitively eliminate arrhythmia. In our case, circumferential pulmonary vein isolation (PVI) could be performed for speech-induced atrial tachycardia to reduce the onset of tachycardia.
Investigation on the psychological impact of grade B or C pancreatic fistula of post pancreatoduodenectomy (PD) on surgeon-in-chief and its related factors
Authors: Zhang Yan , Hu Manqin , Xu Dingwei , Li Xincheng , Li Ao , Huang Jie 
Published: December 2022

Abstract
Background
Postoperative pancreatic fistula (POPF) is the most common complication of pancreatoduodenectomy (PD), and it is the main cause of mortality. A survey was conducted among hepatobiliary pancreatic surgeons to understand the experience of the surgeon-in-chief in PD and POPF, focusing on the psychological impact of POPF on the surgeon-in-chief, related factors, and factors to alleviate psychological pressure.
Methods
A 21-question questionnaire was conducted among hepatobiliary and pancreatic surgeons, adopting the method of a retrospective cross-sectional survey. The questionnaire was distributed through Wechat, and the software platform was questionnaire star. Count data were expressed in absolute values or percentages, and the x2 test was used in univariate analysis.
Results
A total of 97 hepatobiliary and pancreatic surgeons completed the questionnaire. Eighty-six out of the ninety-seven (88.66%) participants were from third-level first-class hospitals, and participants from hospitals below third-level first-class made up 11/97 (11.34%). Ninety-two out of ninety-seven (94.85%) participants felt uneasy, nervous, or anxious after their patients suffered from grade B or C POPF. Participants making 67/97 (69.07%) suffered from sleeplessness, and 56/97(57.73%) participants' decision-making ability for treatment was affected. Participants making a total of 73/97(75.26%) were worried about grade B or C POPF when performing PD again, becoming overly alert.
Conclusions
Whether hepatobiliary and pancreatic surgeons in hospitals of Third-Level First Class or below, whether they have PD experience, and whether they have experienced grade B or C POPF will have a psychological impact on the surgeon-in-chief.
Factors of Intervertebral Disc Rehydration after Percutaneous Endoscopic Lumbar Discectomy
Authors: Lai Juyi , Tan Huangsheng , Li Haokang , Li Jin , Feng Hualong , Lan Zhi Ming , Sun Zhitao , Wang Jian , Fu Yuanfei , Shenghua He 
Published: December 2022

Abstract
Percutaneous endoscopic lumbar discectomy (PELD) is an effective surgical option for lumbar disc herniation (LDH) but is associated with lumbar intervertebral disc rehydration in some patients. However, the incidence of rehydration is not clearly, and only a few studies have discussed this in detail. The study aimed at investigating the factors influencing intervertebral disc rehydration in a series of patients with LDH who underwent PELD. Ninety-six patients with single-level LDH who underwent PELD at Shenzhen Traditional Chinese Medicine Hospital from October 2013 to May 2021 were studied. The relevant data of the patients were recorded such as age, course of disease, sex, body mass index (BMI), diabetes mellitus, hypertension, smoking status, and postoperative exercise; imaging factors such as Modic changes of adjacent vertebrae at surgical level, Pfirrmann grade, degree of disc herniation (Komori classification), and spinal canal morphology; peripheral blood factors such as blood routine parameters, C-reactive protein level, erythrocyte sedimentation rate (ESR), and Th1/Th2 subgroup analysis; and operation-related factors such as surgical approach, length of operation, and amount of blood loss. Univariate analysis and binary logistic regression analysis were conducted to determine the influence of the above parameters on disc rehydration. Our results showed that Young patients with intervertebral disc herniation with a short course of disease, no smoking history, not overweight, no history of diabetes, with intervertebral disc herniation type of Komori Type C, Pfirrmann grade IV, and no adjacent vertebral Modic changes, coupled with high levels of mononuclear cells and TNF-α in peripheral blood, may be more prone to rehydration after PELD.
Oxidative Stress regulates glycogen synthase kinase-3 in Lymphocytes of Diabetes Mellitus Patients with cerebral infarction
Authors: Wang Man , Wang shujin , Qu Zhongsen 
Published: December 2022

Abstract
Diabetes mellitus and cerebral infarction are two major diseases increasing with aging that threaten human health，Oxidative stress is the main cause of diabetes mellitus and acute cerebral ischemic injury.However,It has not been clear how changes in oxidative stress of peripheral lymphocytes affect GSK-3 in DM patients with cerebral infarction.Here,186 patients with DM were enrolled and randomly assigned to DM group(n=89) and DM with cerebral infarction (DM+CI) group (n=97).Eighty-one patients with cerebral infarction were selected as cerebral infarction (CI) group and eighty normal subjects over 50 years were selected as the control group.Superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity,and malondialdehyde (MDA) content in serum were determined by colorimetric assays.Phosphorylation of GSK-3βwas measured by enzyme-linked immunosorbent assay (ELISA).It was found that compared with the control group,the SOD activity and the GSH-Px activity in DM+CI group were decreased,accompanied by higher MDA content.Phosphorylation of GSK-3βwas continuously decreased.Moreover,SOD activity,GSH-P x activity and phosphorylation of GSK-3βin DM +CI group was decreased compared to the CI group and the DM group,while MDA content was increased.We demonstrated that compared with DM and CI group, DM patients with cerebral infarction had a more obvious oxidative stress response and severe oxidative damage. The increased GSK-3βactivity, causing more severe cellular damage, may be one of the mechanisms by which oxidative stress causes DM with cerebral infarction. which might provide a diagnostic and therapeutic approach for DM patients with cerebral infarction.
Peroxisome proliferator-activated receptor gamma (PPARγ) pathway mediates anticancer activity of Britannin, isolated from Inula aucheriana DC., in human gastric cancer cells
Authors: Abdolmohammadi Mohammad Hossein , Roozbehani Maryam , Hamzeloo-Moghadam Maryam , Heidari Fatemeh , Fallahian Faranak 
Published: December 2022

Abstract
Background Researchers are particularly interested in the plants of the genus Inula because of their potent medicinal applications. Britannin, a sesquiterpene lactone isolated from Inula aucheriana DC., exerts potent cytotoxicity towards various human cancers. In this study, we examined the involvement of the PPARγ pathway in the antitumor effect of Britannin against gastric cancer cells. Methods and Results Cytotoxic activity of Britannin was evaluated by MTT assay, followed by Annexin V-FITC/PI staining and caspase-3 activity assay. The expression levels of Nuclear factor kappa B (NF-κB), Inhibitor of nuclear factor kappa B (IκBα), and Peroxisome proliferator-activated receptor γ (PPARγ) were determined by western blotting. Quantitative RT-PCR was used to measure the effect of Britannin on the expression of NF-κB target genes. Our results showed that Britannin inhibited gastric cancer cell growth by inducing apoptotic death. The mRNA and protein levels of PPARγ were significantly increased following treatment with Britannin. The involvement of PPARγ was more confirmed using GW9662, a PPARγ inhibitor. Suppression of NF-κB was also demonstrated by western blot analysis. Down-regulation of Cyclooxygenase-2 (COX-2), Matrix Metalloproteinase 9 (MMP-9), Twist Family BHLH Transcription Factor 1 (TWIST-1), and B-cell lymphoma 2 (Bcl-2) and upregulation of Bcl-2-Associated x (Bax) were also observed in gastric cancer cells treated with Britannin. Conclusions These results imply that activation of the PPARγ signaling pathway through suppression of NF-κB underlies the anti-cancer properties of Britannin in gastric cancer. Therefore, Britannin could be considered for further investigations to explore novel potent anti-cancer drugs.