bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pride and adversity among nurses and physicians during the pandemic in two US healthcare systems: a mixed methods analysis
Authors: Burstyn Igor , Holt Karyn 
Published: March 2022

AbstractOur aims were to examine themes of the most difficult or distressing events reported by healthcare workers during the first wave of COVID-19 pandemic in two US health care systems in order to identify common themes and to relate them to both behavioral theory and measures of anxiety and depression.We conducted a cross-sectional survey during the early phases of the COIVD-19 pandemic in the US. We measured symptoms of anxiety and depression separately, captured demographics, and asked two open-ended questions regarding events that were the most difficult or stressful, and reinforced pride. The open-end questions were independently coded into themes developed by the authors and mapped to factors related to fostering well-being according to the Self-Determination Theory.We recruited 874 nurses and 248 physicians. About a half shared their most distressing experiences as well as those experiences they were most proud of related to their professions. Themes that emerged from these narratives were congruent with prediction of Self-Determination theory that autonomy-supportive experiences will foster pride, while autonomy-thwarting experiences will cause distress. Those who reported distressful events were more anxious and depressed compared to those who did not. Among those who reported incidences that reinforced pride in the profession, depression was rarer compared to those who did not. These trends were evident after allowing for medical history and other covariates in logistic regressions.Causal claims from our analysis should be made with caution due to the research design, a cross-sectional study design. Understanding of perceptions of the pandemic by nurses and physicians may help identify sources of distress and means of reinforcing pride in the professions, thereby helping nurses and physicians cope with disasters, and shape workplace policies during disasters that foster well-being among first responders.No Patient or Public Contribution: We studied physicians and nurses themselves.
Performance of VIDAS® Diagnostic Tests for the Automated Detection of Dengue Virus NS1 Antigen and of Anti-Dengue Virus IgM and IgG Antibodies: a Multicentre, International Study
Authors: Versiani Alice F. , Kaboré Antoinette , Brossault Ludovic , Dromenq Loïc , dos Santos Thayza M.I.L. , Milhim Bruno H.G.A. , Estofolete Cássia F. , Cissé Assana , Sorgho Abel C. , Senot Florence , Tessonneau Marie , Diagbouga Serge , Nogueira Mauricio L. 
Published: March 2022

AbstractDengue is a serious mosquito-transmitted disease caused by the dengue virus (DENV). Rapid and reliable diagnosis of DENV infection is urgently needed in dengue-endemic regions. We describe here the performance evaluation of the CE-marked VIDAS®dengue immunoassays developed for the automated detection of DENV NS1 antigen, and anti-DENV IgM and IgG anti-bodies. A multicenter concordance study was conducted in 1296 patients from dengue-endemic regions in Asia, Latin America, and Africa. VIDAS®dengue results were compared to those of competitor enzyme-linked immunosorbent assays (ELISA). The VIDAS®dengue assays showed high precision (CV ≤ 10.7%) and limited cross-reactivity (≤ 15.4%) with other infections. VIDAS®DENGUE NS1 Ag showed high positive and negative percent agreement (92.8% PPA, 91.7% NPA) in acute patients within 0–5 days of symptom onset. VIDAS®Anti-DENGUE IgM and IgG showed a moderate to high concordance with ELISA (74.8% to 90.6%) in post-acute and recovery patients. PPA was further improved in combined VIDAS®NS1/IgM (96.4% in 0–5 days acute patients) and IgM/IgG (91.9% in post-acute patients) tests. Altogether, the VIDAS®dengue NS1, IgM and IgG assays performed well, either alone or in combination, and should be suitable for the accurate diagnosis of DENV infection in dengue-endemic regions.
Comparison of co-immunoprecipitation techniques for effective identification of SNTA1 interacting proteins in breast cancer cells
Authors: Sajood Saima , Prasad T. S. Keshava , Bhat Basharat , Bhat Zuhiab F. , Shah Riaz A , Bhat Hina F 
Published: March 2022

AbstractDiverse signal transduction pathways involve thousands of proteins acting in concert that contribute to breast cancer pathology. SNTA1 has emerged as a common link that functions to wire various complex signaling circuits within the cell. In our study we used endogenous SNTA1 protein as bait in pull-down assays to identify SNTA1 interactome from breast cancer cell extract. We compared the utility of two co-immunoprecipitation techniques conventional co-IP and cross-linking co-IP coupled to mass spectrometry (IP–MS) that intriguingly generated valuable inventory of SNTA1 associated proteins in breast cancer cells that may or may not interact physically but contribute to shared functions in breast cancer pathology. We observed that Pierce cross-link IP kit enhanced sensivity of the analysis as it decreased the number of background proteins with a remarkable increase in identified list of proteins. We have also observed that different approaches are able to consistently detect the same signaling outcome as observed by Gene Ontology. Identification of interacting partners of SNTA1 in breast cancer cell lines may depict the possible signaling mechanism of this protein in molecular pathogenesis of breast cancer and its possible use as a therapeutic target.
clampFISH 2.0 enables rapid, scalable amplified RNA detection in situ
Authors: Dardani Ian , Emert Benjamin L. , Goyal Yogesh , Jiang Connie L. , Kaur Amanpreet , Lee Jasmine , Rouhanifard Sara H. , Alicea Gretchen M. , Fane Mitchell E. , Xiao Min , Herlyn Meenhard , Weeraratna Ashani T. , Raj Arjun 
Published: March 2022

AbstractRNA labeling in situ has enormous potential to visualize transcripts and quantify their levels in single cells, but it remains challenging to produce high levels of signal while also enabling multiplexed detection of multiple RNA species simultaneously. Here, we describe clampFISH 2.0, a method that uses an inverted padlock design to efficiently detect and exponentially amplify signals from many RNA species at once, while also reducing time and cost compared to the prior clampFISH method. We leverage the increased throughput afforded by multiplexed signal amplification and sequential detection by demonstrating the ability to detect 10 different RNA species in over 1 million cells. We also show that clampFISH 2.0 works in tissue sections. We expect the advantages offered by clampFISH 2.0 will enable many applications in spatial transcriptomics.
Identification of Aspergillus niger aquaporins involved in hydrogen peroxide signaling
Authors: Laothanachareon Thanaporn , Asin-Garcia Enrique , Volkers Rita J.M. , Tamayo-Ramos Juan Antonio , Schaap Peter J. 
Published: March 2022

ABSTRACTAspergillus niger is a robust microbial cell factory for organic acids production. However, the regulation of many industrially important pathways is still poorly understood. Recently the regulation of glucose oxidase (GOx) expression system involved in the biosynthesis of gluconic acid has been uncovered. Hydrogen peroxide, a by-product of the enzymatic conversion of glucose to gluconate, has a pivotal role as a signaling molecule in the induction of this system. In this study, facilitated diffusion of hydrogen peroxide via aquaporin water channels (AQPs) was studied. AQPs are transmembrane proteins of the major intrinsic proteins (MIPs) superfamily. In addition to water and glycerol, they may also transport small solutes such as hydrogen peroxide. Accordingly, the genome sequence of A. niger N402 was screened for putative AQPs. Seven were found which could be classified in three main groups. Their ability to facilitate diffusion of hydrogen peroxide was identified using yeast phenotypic growth assays and by studying AQP gene knock-outs in A. niger. Complementing from both yeast and A. niger experiments, the X-intrinsic protein AQPF plays apparent roles in facilitating hydrogen peroxide transport across cellular membrane.IMPORTANCEAquaporins (AQP) are integral membrane proteins that facilitate transfer of water, small solutes and hydrogen peroxide across the plasma membrane. As they have conserved structural features, it is not difficult to detect their presence in fungal genomes. Their specific roles in fungi are however less understood. Previously we have elucidated the role of the hydrogen peroxide as a second messenger in the induction and regulation of the expression of glucose oxidase involved in the extracellular conversion of glucose in gluconate (1). Here we identified the involvement of AQPF, a member of the X-intrinsic protein class in facilitating transport of the hydrogen peroxide signal across the cell membrane.
Instance segmentation of mitochondria in electron microscopy images with a generalist deep learning model
Authors: Conrad Ryan , Narayan Kedar 
Published: March 2022

AbstractMitochondria are extremely pleomorphic organelles. Automatically annotating each one accurately and precisely in any 2D or volume electron microscopy (EM) image is an unsolved computational challenge. Current deep learning-based approaches train models on images that provide limited cellular contexts, precluding generality. To address this, we amassed a highly heterogeneous ∼1.5 x 106 image 2D unlabeled cellular EM dataset, and segmented ∼135,000 mitochondrial instances therein. MitoNet, a model trained on these resources, performs well on challenging benchmarks and on previously unseen volume EM datasets containing tens of thousands of mitochondria. We release a new Python package and napari plugin, empanada, to rapidly run inference, visualize, and proofread instance segmentations.
Does office aesthetics drive job choice? Boosting employee experience and well-being perception through workplace design
Authors: Ronda Lorena , de Gracia Elena 
Published: March 2022

PurposeDrawing from experiential theory and decision-making theory, this article aims to posit that workplace aesthetics acts as a driver for job choice when included with an employment offer. Whilst organisational literature has recognised that office experiential cues in general, and aesthetics in particular, affect employee performance and well-being, employer attractiveness scales have not yet incorporated office aesthetics as a component of job-offer choice.Design/methodology/approachA choice-based conjoint (CBC) experiment was conducted to estimate the weighted utilities of three aesthetic and three non-aesthetic employer attributes. Subsequently, the attributes' importance in the job choice decision was estimated.FindingsThe results indicate that aesthetic attributes in the workplace can be equally important in the decision-making process as non-aesthetic attributes and that aesthetic attributes deliver as much utility as non-aesthetic attributes in driving job choice.Practical implicationsThese conclusions are relevant for Human Resource (HR) managers engaged in crafting job offers, who should consider that employees may improve their assessment of a job offer as a result of superior organisational aesthetics demonstrated during the recruitment process as well as in contexts where employees would be expected to combine remote and office-based work.Originality/valueThe present study represents a novel approach to understanding job applicants' preferences for aesthetic elements in the workplace. The results suggest that the workplace experience is relativistic and that considering applicants' latent preferences is crucial when designing efficient job offers.
Digitalization, innovation capabilities and absorptive capacity in the Swedish real estate ecosystem
Authors: Vigren Olli , Kadefors Anna , Eriksson Kent 
Published: March 2022

Purpose
The purpose of this paper is to increase the knowledge of real estate firms’ capabilities to innovate and, consequently, their capacity to absorb new innovations and benefit from digital technologies in an ecosystem context.

Design/methodology/approach
The results are based on 32 interviews with representatives of Swedish real estate owners, real estate owner industry associations and suppliers of digital technology to real estate owners. The data are interpreted using theories on absorptive capacity (i.e. the capacity to absorb new innovations), innovation capabilities and innovation ecosystems.

Findings
The real estate owners, technology suppliers and real estate owner industry associations have expanded their innovation capabilities and reshaped their innovation ecosystem by initiating a number of different digitalization activities; for example, the development of new IT systems, digital platforms, services and business models. The absorptive capacity has been improved as the organizations have changed routines and structures related to innovation, and they have taken on new roles related to digitalization and innovation, making them better able to absorb new innovations. Also, this paper identifies several drivers and obstacles to digitalization in the real estate sector.

Research limitations/implications
The increased capabilities related to digitalization can lead to better absorptive capacity on an individual firm level, which can contribute to the overall development of these firms in a longer-term. Also, new capabilities may lead to better absorptive capacity in the real estate sector at large, as firms may benefit from each other’s capabilities through collaboration. The limitations are that this study does not interview tenants or facility management firms and that the findings represent the context of the Swedish real estate market.

Originality/value
This paper investigates innovation capabilities, absorptive capacity and innovation ecosystems of real estate owners, their technology suppliers and real estate owner industry associations on the organizational level and on the sector level, into which there is little previous research. Also, this paper highlights the novelty of digitalization as a phenomenon in the sector.
A qualitative study proposing service quality dimensions for video-on-demand services through over-the-top medium
Authors: Datta Satyaki , Utkarsh Utkarsh 
Published: March 2022

Purpose
The behaviour of audience, consuming video entertainment, has changed intensely over the years. Lately, the consumers have increasingly preferred to watch video programs, through video-on-demand services through over-the-top medium. The service is novel and the consumer’s perception of the service quality is not well explored. As extant literature considers service quality as the construct to determine the sustained growth of a service, the present study has attempted to explore the dimensions to measure service quality of video-on-demand services.

Design/methodology/approach
The authors conducted qualitative, semi-structured interviews and focus group discussions amongst the user of the video-on-demand service. The qualitative data was content analysed to furnish thematic dimensions.

Findings
The study reveals thematic attributes perceived as dimensions to measure service quality of video-on-demand services.

Research limitations/implications
Considering the exploratory nature of the study, the themes proposed might seem nascent. Hence, it was the authors’ discretion to stop expanding the respondent sample to avoid data saturation. A quantitative establishment of the service quality dimensions was beyond the scope of the current research and would follow in a different study.

Originality/value
The objective of the study is to qualitatively explore service quality dimensions of video-on-demand services. In pursuit of that, the current study explored the consumers’ excerpts, content analysed the data and furnished several themes perceived as service quality dimensions in this context. Such a detailed approach is uncommon in this context.
Wines from the hinterland: comparing availability of local wines on wine lists in two New Zealand tourism destinations
Authors: Hill Rory , Fountain Joanna 
Published: March 2022

Purpose
This paper aims to situate restaurant experiences and in particular the wines available on wine lists, within the wider context of wine tourism. This is done by examining the wine lists of restaurants in two New Zealand destinations, focusing in particular on the showcasing of “local wines” and the factors behind these offerings, and outlines the potential implications for hospitality managers and a wider academic audience.

Design/methodology/approach
The population of restaurants in each destination was identified using online directories, from which a sample of wine lists, comprising 84 in Christchurch and 43 in Queenstown, was systematically analysed to identify number of wines, regional origin, price and other information. Following this, key informant interviews in restaurants in each destination explored decision-making factors in stocking local wines, including consumer base, existing networks and reputation and additional challenges and opportunities.

Findings
Restaurants in each destination offered more New Zealand than foreign wines on their lists, though significant regional differences are apparent. Queenstown restaurants offered slightly fewer imported wines and significantly more local (Central Otago) wines than Christchurch restaurants. The global awareness of Central Otago pinot noir is a factor in this wine list representation, but there are also other influences, including the greater concentration of overseas visitors (pre-pandemic) and more significant visibility and greater opportunities for wine tourism experiences within the destination.

Originality/value
This paper represents an important addition to academic research on wine marketing in the on-premise sector of emerging wine regions. This paper also highlights the potential significance of restaurant meals – including wine choices – in overall wine destination experiences and demonstrates differences in approach between restaurants in wine regions of similar size but with different reputations, international visitation and wine tourism infrastructure.
Hijacking the auction – seller's or buyer's curse?
Authors: Flått Marte , Olaussen Jon Olaf , Oust Are , Sønstebø Ole Jakob 
Published: March 2022

PurposeThis paper aims to investigate the likelihood and price effects of auction hijacking – transactions conducted before a planned auction – in the residential real estate market.Design/methodology/approachUsing a sample of 84,203 residential properties in Oslo, Norway for the period 2007–2017, the authors employ a probit sales choice model to study the likelihood of auction hijacking and hedonic models, fixed effects models and propensity score matching to investigate the price effects.FindingsThe authors find that auction hijacking is more likely to occur in periods of higher market activity and that hijacked auction properties sell at a premium of approximately 4% compared with properties sold at regularly conducted auctions. One possible explanation for the premium could be that risk aversion among buyers leads to a higher likelihood of hijacking offers and higher prices due to the risk reduction premium that sellers can extract.Originality/valueFor most households, buying a home is an investment of great economic significance, and understanding the different aspects of the auction is paramount for both buyers and sellers. Policymakers need to be aware of the market effects from auction hijacking and determine whether restrictions should be introduced.
Tension stiffening model for the finite element analysis of composite floor systems exposed to fire
Authors: Martinez Jason , Jeffers Ann 
Published: March 2022

PurposeA methodology for producing an elevated-temperature tension stiffening model is presented.Design/methodology/approachThe energy-based stress–strain model of plain concrete developed by Bažant and Oh (1983) was extended to the elevated-temperature domain by developing an analytical formulation for the temperature-dependence of the fracture energy Gf. Then, an elevated-temperature tension stiffening model was developed based on the modification of the proposed elevated-temperature tension softening model.FindingsThe proposed tension stiffening model can be used to predict the response of composite floor slabs exposed to fire with great accuracy, provided that the global parameters TS and Kres are adequately calibrated against global structural response data.Originality/valueIn a finite element analysis of reinforced concrete, a tension stiffening model is required as input for concrete to account for actions such as bond slip and tension stiffening. However, an elevated-temperature tension stiffening model does not exist in the research literature. An approach for developing an elevated-temperature tension stiffening model is presented.
Molecular insights into human taste perception and umami tastants: A review
Authors: Diepeveen Johan , Moerdijk‐Poortvliet Tanja C. W. , van der Leij Feike R. 
Published: March 2022

AbstractUnderstanding taste is key for optimizing the palatability of seaweeds and other non‐animal‐based foods rich in protein. The lingual papillae in the mouth hold taste buds with taste receptors for the five gustatory taste qualities. Each taste bud contains three distinct cell types, of which Type II cells carry various G protein‐coupled receptors that can detect sweet, bitter, or umami tastants, while type III cells detect sour, and likely salty stimuli. Upon ligand binding, receptor‐linked intracellular heterotrimeric G proteins initiate a cascade of downstream events which activate the afferent nerve fibers for taste perception in the brain. The taste of amino acids depends on the hydrophobicity, size, charge, isoelectric point, chirality of the alpha carbon, and the functional groups on their side chains. The principal umami ingredient monosodium l‐glutamate, broadly known as MSG, loses umami taste upon acetylation, esterification, or methylation, but is able to form flat configurations that bind well to the umami taste receptor. Ribonucleotides such as guanosine monophosphate and inosine monophosphate strongly enhance umami taste when l‐glutamate is present. Ribonucleotides bind to the outer section of the venus flytrap domain of the receptor dimer and stabilize the closed conformation. Concentrations of glutamate, aspartate, arginate, and other compounds in food products may enhance saltiness and overall flavor. Umami ingredients may help to reduce the consumption of salts and fats in the general population and increase food consumption in the elderly.