bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of immediate postoperative hemodynamic and laboratory values in predicting Norwood admission outcomes
Authors: Loomba Rohit , Dyamenahalli Umesh , Savorgnan Fabio , Acosta Sebastian , Villarreal Enrique , Farias Juan , Wong Joshua , Flores Saul 
Published: May 2022

Abstract

Background
The primary objective of this study was to determine whether or not hemodynamic parameters and laboratory values at the time of admission to the pediatric cardiac intensive care unit after the Norwood operation were associated with a composite outcome of either need for extracorporeal membrane oxygenation or inpatient mortality.
Methods
This was a single-center retrospective study of infants with functionally univentricular hearts admitted to intensive care after the Norwood procedure from January 2011 to January 2020. Data were obtained at a single point(after a Norwood procedure) and then compared between two subsets of patients based on the presence or not of the composite outcome of interest. In univariate and multiple regression analyses, a series of receiver operator curves were generated to assess the relationship between the variables of interest and the composite outcome.
Results
Eight(7.6%) experienced the composite outcome out of a total of 104 patients. Those who experienced the composite endpoint had significantly higher oxygen extraction ratio (0.43 vs. 0.31, p = 0.01), lower systemic blood flow (2.5 L/min versus 3.1 L/min, p = 0.01), and higher systemic vascular resistance (20.2 indexed woods units versus 14.8 indexed woods units, p = 0.01). Those with systemic blood flow of less than 2.5 L/min/m2 had a 17% risk of experiencing the composite endpoint AUC = 0.79. Those with systemic vascular resistance of greater than 19 indexed woods units had a 22% risk of experiencing the composite endpoint AUC 0.80.
Conclusion
Systemic blood flow and systemic vascular resistance are independently associated with this composite outcome.
Fine-scale habitat suitability and connectivity analysis for the core populations of Yellow-spotted mountain pond-breeding newt (Neurergus derjugini) in the west of Iran and east of Iraq
Authors: Karami Peyman , tavakoli Sajad , Esmaeili Mina 
Published: May 2022

Abstract
The evolutionary characteristics of amphibians, especially those more water-dependent, have faced the study of habitat connectivity the challenge of Movement Context (MC), a context for which water and moisture are an integral part. This fine-scale study was conducted to evaluate the distribution of N. derjugini and identify potential displacement paths between the population cores, using the physical elements of the landscape playing an MC role. The distribution modeling was performed by MaxEnt and the inverse of the habitat suitability map was used as the cost map. The connection of population cores was modeled by the Linkage Mapper toolbox. The MC of the population cores was set in the drainage basins regarding the slope position and landform classes. Three scenarios were considered. In Scenario 1, drainage basins, in Scenario 2, the valleys, and in Scenario 3, canyons, shallow valleys, headwaters, and u-shaped valleys were considered MCs. According to the results, slope and altitude variability had the highest effect on the distribution of N. derjugini. The results showed that the proxy did not work well in Scenario 1. Compared to Scenario 2, the connection paths of Scenario 3 were more compatible with the ecological and biological characteristics of the species. fine-scale modeling could lead to reliable results for the displacement of MC-dependent species. The connectivity of the physical structure of landscapes plays a critical role in the connectivity of population cores. Proper distribution of population cores adjacent to each other in valleys and waterways can be considered a successful step in communication.
Cellular uptake and localization of Hematoporphyrin derivatives in lung adenocarcinoma A549, squamous carcinoma H520 and small cell carcinoma H446 cell lines
Authors: Ma Yijiang , Ding Xiaoqian , Sui Aihua , Yang Xiaohui , Cui Shichao , Cao Yiwei , Lin Cunzhi 
Published: May 2022

Abstract
Objective
To select bronchial epithelial BEAS-2B cell line as control, cellular uptake and localization of Hematoporphyrin derivatives (HPD) were compared between normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (lung adenocarcinoma A549, lung squamous carcinoma H520 and lung small cell carcinoma H446), as well as among lung cancer cell lines.
Methods
The standard curve of HPD was drawn based on the fluorescence intensity of different concentrations of HPD measured by a multifunctional microplate reader. Cellular uptake of HPD was quantified by fluorescence intensity with a multifunctional microplate reader and flow cytometry. Cellular distribution of HPD was observed and compared by laser scanning confocal microscopy.
Results
In a certain concentration range, HPD concentration was positively correlated with fluorescence intensity. Cellular uptake of HPD increased with time. HPD was rapidly accumulated in BEAS-2B, A549 and H446 cells after incubating with HPD for 12h, while the uptake rate of HPD was slow in H520 cells and accelerated after 24h. In the 24h and 48h experimental groups, the average fluorescence intensity in the four cells was significantly different (P < 0.0001), and the average fluorescence intensity in A549 cells was higher than that in H446, BEAS-2B, and H520 cells. However, the mean fluorescence intensity of H446 and BEAS-2B cells had no statistical difference (P = 0.0766 > 0.05), and both were higher than those of H520 cells (P < 0.001). The four cell lines showed red fluorescent signals of varying intensities, and all were distributed in the cytoplasm.
Conclusion
In vitro experiments show that there are differences in HPD uptake between normal cell lines and lung cancer cell lines and between different types of lung cancer cell lines. The ability to take HPD in A549 cells is the strongest, followed by BEAS-2B cells and H446 cells, and the uptake ability of HPD in H520 cells is the weakest. The photosensitizer HPD is located in the cytoplasm of both cell lines, showing a punctate distribution.
Entanglement and quantum correlations in the XX spin-1/2 honeycomb lattice
Authors: Satoori Sahar , Mahdavifar Saeed , Vahedi Javad 
Published: May 2022

Abstract
The ground state phase diagram of the dimerized spin-1/2 XX honeycomb model in presence of a transverse magnetic field (TF) is known. With the absence of the magnetic field, two quantum phases, namely, the Néel and the dimerized phases have been identified. Moreover, canted Néel and the paramagnetic (PM) phases also emerge by applying the magnetic field. In this paper, using two complementary numerical exact techniques, Lanczos exact diagonalization, and Density matrix renormalization group (DMRG) methods, we study this model by focusing on the quantum correlations, the concurrence, and the quantum discord (QD) among nearest-neighbor spins. We show that the quantum correlations can capture the position of the quantum critical points in the whole range of the ground state phase diagram consistent with previous results. Although the concurrence and the QD are short-range, informative about long-ranged critical correlations. In addition, we address a “magnetic-entanglement” behavior that starts from an entangled field around the saturation field.
Effect of Inferior Rectus Muscle Recession on Intraocular Pressure in Thyroid Eye Disease Patients with Ocular Hypertension
Authors: Wu Yu-Chieh , Wang Chun-Yuan , Wei Li-Chen 
Published: May 2022

Abstract
● BACKGROUND: To study the effect of inferior rectus muscle recession on intraocular pressure (IOP) in thyroid eye disease (TED) patients with ocular hypertension.● METHODS: This is a retrospective, observational case series. The medical records of patients with ocular hypertension, despite use of antiglaucoma medications, and restrictive myopathy secondary to thyroid eye disease who underwent inferior rectus muscle recession from January 1, 2016 through December 31, 2021 were reviewed and analyzed retrospectively. Seven eyes from six patients met the criteria and were included in this study. All patients were examined at Taichung Veterans General Hospital, a tertiary referral center. The main outcome measure was IOP readings obtained before and after surgery in both primary gaze (hypotropia) and upgaze (or attempted upgaze).● RESULTS: A statistically significant decrease in IOP in primary gaze (hypotropia) and upgaze (or attempted upgaze) was noted after inferior rectus muscle recession. The median IOP before surgery was 24.0 mmHg in primary gaze and 30.5 mmHg in upgaze. After strabismus surgery, the median IOP after one week was 18.0 mm Hg in primary gaze and 20.0 mmHg in upgaze; the median IOP one month after surgery was 15.0 mmHg in primary gaze and 16.5 mmHg in upgaze; the median IOP 3 months after surgery was 12.5 mmHg in primary gaze and 13.5 mmHg in upgaze; the median IOP 12 months after surgery was 15.5 mmHg in primary gaze (overall P = .001) and 17.0 mmHg in upgaze (overall P = .001). All eyes were treated with three to four antiglaucoma medications before the surgery. Four eyes discontinued all preoperative medications within 3 months after the surgery. The other three eyes kept using preoperative medications.● CONCLUSIONS: Inferior rectus muscle recession resulted in a significant and sustained reduction in IOP in patients with ocular hypertension and restrictive myopathy secondary to thyroid eye disease.
Human Genetics Education as Part of the Japanese Cancer Education Comprehensive Support Progect
Authors: Yamada-Kurebayashi Fumi , Sasaki Motoko , Kuga Asami , Kojima Risa , Miyake Hidehiko 
Published: May 2022

Abstract
In Japan, cancer education has been initiated with children as a measure against cancer. Cancer genome medicine, which is a social implementation, includes aspects of genetic medicine. For this reason, it is assumed that content related to "genetics" is also necessary in cancer education. To investigate the actual situation regarding the teaching of genetics in cancer education, we conducted a questionnaire survey of schoolteachers involved in cancer education; these schoolteachers belonged to the model school of the Cancer Education Comprehensive Support Project. Regarding genetics content, we asked questions related to two aspects: “the molecular genetic mechanisms of cancer” and “the phenomenon of sharing cancer in the family.” The results showed that about 60% of the teachers had experience teaching content related to “the molecular genetic mechanisms of cancer” and “the phenomenon of sharing cancer in the family.” While many teachers felt that teaching genetics in cancer education was necessary, they also felt that there were difficulties in doing so: 65.2% for content related to "the molecular genetic mechanisms of cancer " and 70.8% for that related to “the phenomenon of sharing cancer in the family.” It is important to properly treat cancer as a genetic disease, and it is necessary to examine government curriculum guidelines and establish a collaborative system among other subjects. In addition, the involvement of specialists in genetic medicine and psychosocial support is expected to improve teachers’ genetic literacy as well as to communicate with students with consideration for their family history.
PDS5A and PDS5B differentially affect gene expression without altering cohesin localization across the genome
Authors: Arruda Nicole L. , Bryan Audra F. , Dowen Jill M. 
Published: May 2022

Abstract
Background Cohesin is an important structural regulator of the genome, regulating both three-dimensional genome organization and gene expression. The core cohesin trimer interacts with various HEAT repeat accessory subunits, yielding cohesin complexes of distinct compositions and potentially distinct functions. The roles of the two mutually exclusive HEAT repeat subunits PDS5A and PDS5B are not well understood. Results Here, we determine that PDS5A and PDS5B have highly similar localization patterns across the mouse embryonic stem cell (mESC) genome and they show a strong overlap with other cohesin HEAT repeat accessory subunits, STAG1 and STAG2. Using CRISPR/Cas9 genome editing to generate individual stable knockout lines for PDS5A and PDS5B, we find that loss of one PDS5 subunit does not alter the distribution of the other PDS5 subunit, nor the core cohesin complex. Both PDS5A and PDS5B are required for proper gene expression, yet they display only partially overlapping effects on gene targets. Remarkably, gene expression following dual depletion of the PDS5 HEAT repeat proteins does not completely overlap the gene expression changes caused by dual depletion of the STAG HEAT repeat proteins, despite the overlapping genomic distribution of all four proteins. Furthermore, dual loss of PDS5A and PDS5B decreases cohesin association with NIPBL and WAPL, reduces SMC3 acetylation, and does not alter overall levels of cohesin on the genome. Conclusions Together, this work reveals the importance of PDS5A and PDS5B for proper cohesin function. Loss of either subunit has little effect on cohesin localization across the genome yet PDS5A and PDS5B are differentially required for gene expression. This work advances our understanding of how HEAT repeat proteins regulate cohesin function.
A Distinctive Routing Protocol for Energy Distribution of Nodes in Wireless Sensor Network
Authors: Rahman Maksuda , Tasneem Zareen , Tafannum Farissa , Rahman Md. Mahbubur , Shahriar Mohammad Rafid , Islam Monirul Islam 
Published: May 2022

Abstract
Wireless Sensor Network (WSN) consists of sensor nodes and base station. In WSN, reduction of energy and extension of network lifetime are important facts. In this paper, LEACH (Low-Energy Adaptive Clustering Hierarchy) which is a hierarchical routing protocol, is improved. Weight exponent of network, residual energy of the nodes, SCHs (Supporting Cluster Head) and also distance to CH (Cluster head) and SCH are considered in the proposed scheme which is RF-LEACH or refined LEACH. Based on network lifetime a comparison between LEACH, LEACH-SCH and RF-LEACH protocol is analyzed. The implementation is done using MATLAB 2013a. From the simulation results, it can be concluded that the proposed scheme is better than LEACH and LEACH-SCH.
A cross-sectional analysis from the PAC-19QoLReg on the association between Quality of Life (QoL) of long COVID-19 patients and their disease duration
Authors: Jandhyala Ravi , Lin Yuhui 
Published: May 2022

Abstract
Background: The COVID-19 pandemic has been recognized for its long-term detrimental effects on the human population. The aim of this study was to report on the quality of life (QoL) from the interim analysis of COVID-19 positive patients registry data (PAC-19QoLReg) at baseline.Method: Self-declared patients who received COVID-19 positive diagnosis and/or had exhibited symptoms of lagged effects from post-COVID-19 for a duration of at least 28 days were recruited into the PAC-19QoLReg in December 2020 via social media, broadcasting platforms community forums and by word-of-mouth.Results: At primary recruitment, a total of 107 participants were recruited into the study. However, this interim data reports on N=74 long COVID-19 patients who have completed their baseline PAC-19QoL; Survey 1. The generalized linear model (GLM) Poisson regression was applied in this study to examine the association between exposure duration to COVID-19 and PAC-19QoL scores. The finalized model was adjusted for truncation time and age at recruitment. Truncation time was considered for its contribution in the delayed effects to complete the questionnaire upon recruitment, and such postponement varied among participants. The findings demonstrate that all participants were likely to recover in their QoL scores with increasing time since being diagnosed with COVID-19. The model underwent an elective statistical adjustment with the UK-only participants, and with truncation time at 0-14 and beyond 14 days. Participants who took >14 days to complete the survey were likely to recover in QoL scores than <=14 days. Conclusion: Findings from this study suggests a disproportional impact on patients by gender, age, and study country was observed, emphasizing that an awareness of patient-specific factors can assist clinicians in identifying those who may be at risk for diminished QoL as they progress. Furthermore, psychological adaptation to disease symptoms upon COVID-19 diagnosis was observed among the participants .Trial registration: ClinicalTrials.gov Identifier NCT04586413; 14th October 2020
Comparison of Mainstream End Tidal Carbon Dioxide on Y-Piece Side Versus Patient Side of Heat and Moisture Exchanger Filters in Critically Ill Adult Patients: a prospective observational study
Authors: Tamashiro Satoshi , Nakayama Izumi , Gibo Koichiro , Izawa Junichi 
Published: May 2022

Abstract
Introduction: Whether HMEF impairs the accuracy of mainstream EtCO2 measured on the Y-piece (filtered) side of HMEF compared to that on the patient (unfiltered) side is unclear.Methods: We conducted a prospective observational method comparison study in a teaching hospital in Japan between July 2019 and December 2019. Critically ill adult subjects receiving mechanical ventilation with HMEF were included. We performed a noninferiority comparison of the accuracy of EtCO2 measurements on the two sides of HMEF. The accuracy was measured by the absolute difference between PaCO2 and EtCO2. We set the non-inferiority margin at +1 mmHg in accuracy difference between the Y-piece side and the patient side of HMEF. We also assessed the agreement between PaCO2 and EtCO2 using Bland-Altman analysis.Results: Among thirty-seven subjects, the accuracy difference was -0.14 mmHg (95% CI, -0.58–0.29), and the upper 95% CI limit did not exceed the predefined margin of + 1 mmHg, establishing non-inferiority of EtCO2 on the Y-piece side of HMEF (P for non-inferiority <0.001). In the Bland-Altman analyses, 95% limits of agreement between PaCO2 and EtCO2 were similar in both sides of HMEF (Y-piece side, -8.67–+10.65 mmHg; patient side, -8.93–+10.67 mmHg).Conclusions: The accuracy of mainstream EtCO2 measurements on the Y-piece side of HMEF was noninferior to that on the patient side in critically ill adult subjects. Mechanically ventilated adult subjects could be accurately monitored with mainstream EtCO2 on the Y-piece side of the HMEF unless their tidal volume was extremely low.
Reactive and acute inflammatory microvasculopathy in 36 COVID-19 autopsy brains
Authors: Rhodes Roy H. , Love Gordon L. , Lameira Fernanda Da Silva , Shahmirzadi Maryam Sadough , Fox Sharon E. , Heide Richard S. Vander 
Published: May 2022

Abstract
Background: Hypercytokinemia, the renin-angiotensin system (RAS), and hypoxia are implicated in brain morbidity in COVID-19. There is little evidence of direct SARS-CoV-2 brain infection, although focal microvascular infection and ‘planted’ antigens have been suggested.Methods: Histopathology of the brains from 36 consecutive autopsies of patients who were RT-PCR positive for SARS-CoV-2 was studied. Immunostaining for serum proteins, complement components and virus, as well as viral in-situ hybridization, were employed. The Mann-Whitney U test was used to compare activation of complement in brain microvessels in COVID-19 cases with immunostaining findings in pre-pandemic autopsy brains.Results: Neuropathologic findings in this COVID-19 cohort identified widespread reactive microvasculopathy (ectasia, mural distortion, and intussusceptive arborization) and acute intraluminal neutrophilic endotheliitis in the microcirculation in all 36 cases. Prominent vascular neutrophilic transmural migration was found in several cases where it was best identified in larger microvessels. In 25 cases there was acute microcirculatory perivasculitis. Activation of complement components, which included membrane attack complex, was significantly higher in microvascular walls in the COVID-19 cohort than in pre-pandemic cases.Conclusions: The literature suggests that in COVID-19 patients reactive microvasculopathy most likely originates from hypoxia, hypercytokinemia, and RAS dysfunction, while direct and indirect virus-induced factors may contribute. Acute endotheliitis, transmural migration, and acute perivasculitis constitute the early phase of type 3 hypersensitivity vasculitis in all of the cohort cases. The presence of activated complement components in microvascular walls in COVID-19 autopsy cases compared to controls is consistent with type 3 hypersensitivity vasculitis. Viral antigen in or ‘planted’ on microvessels or other antigen-antibody complexes could cause of this type of autoimmune vasculitis proximate to death. Coupling of neurogliovascular units could be compromised, if only temporarily in various brain regions, during the progression (initiation to healing) of these microvascular findings even in the absence of thrombosis or mural dehiscence. However, no specific neurological alteration in this cohort can be attributed directly to specific histopathologic findings.
Medial versus lateral camera port placement for robotic nephron-sparing surgery: Evaluating surgical complications and outcomes
Authors: Olsen Jamie , Olsen Daniel , Hale Nathan , Deem Samuel , Deslich Stacie 
Published: May 2022

Abstract
IntroductionAs the growing popularity of robotic assisted laparoscopic procedures for the treatment of renal cancer increases, there exists a variation in surgical technique among institutions and surgeons alike. One variation that exists in robotics is the anatomical placement of the camera port (medial versus lateral camera port placement). The purpose of this study is to evaluate surgical complications and outcomes in comparison to site of camera port placement during nephron-sparing surgery in an academic setting. MethodsOver a three-year period, outcomes for all robotic surgeries for renal cancer were examined. A total of 229 cases were discovered. Patient demographics and comorbidities were analyzed along with perioperative surgical data including location of camera port, surgery length, warm ischemia time, blood loss, pathological tumor margins, tumor size, length of stay and laboratory data. Outcomes134 patients had surgery performed with lateral camera port placement versus 95 patients with medial camera port placement. Operative time was significantly lower with an average operative time of 165.8 minutes for the lateral group versus 209.1 minutes in the medial group (p <0. 0001). Warm ischemia time was also less in the lateral group with an average of 11 minutes versus 15.5 minutes for the medial group (p <0. 0001). Blood loss was less in the lateral camera port group with an average of 158.2mL (+/- 196.5mL) versus 248.6mL in the medial group (+/- 252.6) (p=0.0040). Drain use, positive surgical margin rate, transfusion rate, conversion to radical nephrectomy, change in preoperative versus postoperative creatinine and glomerular filtration rate and length of hospital stay did not statistically differ.ConclusionLateral camera port placement is associated with decreased operative time and warm ischemia time in this series. There may be certain laparoscopic advantages through a better visualization of surgical anatomy, thus allowing for faster extirpation of renal lesions and decrease in surgical time. These advantages may result in better long-term renal function and decreased clinical sequela from chronic kidney disease.