bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical application of CSF biomarkers for Alzheimer's disease: From rationale to ratios
Authors: Bouwman Femke H. , Frisoni Giovanni B. , Johnson Sterling C. , Chen Xiaochun , Engelborghs Sebastiaan , Ikeuchi Takeshi , Paquet Claire , Ritchie Craig , Bozeat Sasha , Quevenco Frances‐Catherine , Teunissen Charlotte 
Published: April 2022

AbstractBiomarker testing is recommended for the accurate and timely diagnosis of Alzheimer's disease (AD). Using illustrative case narratives we consider how cerebrospinal fluid (CSF) biomarker tests may be used in different presentations of cognitive impairment to facilitate timely and differential diagnosis, improving diagnostic accuracy, providing prognostic information, and guiding personalized management in diverse scenarios. Evidence shows that (1) CSF ratios are superior to amyloid beta (Aβ)1‐42 alone; (2) concordance of CSF ratios to amyloid positron emission tomography (PET) is better than Aβ1‐42 alone; and (3) phosphorylated tau (p‐tau)/Aβ1‐42 ratio is superior to p‐tau alone. CSF biomarkers are recommended for the exclusion of AD as the underlying cause of cognitive impairment, diagnosis of AD at an early stage, differential diagnosis of AD in individuals presenting with other neuropsychiatric symptoms, accurate diagnosis of AD in an atypical presentation, and for clinical trial enrichment.Highlights
Cerebrospinal fluid (CSF) Alzheimer's disease (AD) biomarker testing may be underused outside specialist centers.
CSF biomarkers improve diagnostic accuracy, guiding personalized management of AD.
CSF ratios (amyloid beta [Aβ]1‐42/Aβ1‐40 and phosphorylated tau/Aβ1‐42) perform better than single markers.
CSF ratios produce fewer false‐negative and false‐positive results than individual markers.
CSF biomarkers should be included in diagnostic work‐up of AD and mild cognitive impairment due to AD.
Coastal infrastructure alters behavior and increases predation mortality of threatened Puget Sound steelhead smolts
Authors: Moore Megan E. , Berejikian Barry A. 
Published: April 2022

AbstractFundamental movements of migratory species can be substantially influenced by marine habitat disruptions caused by coastal infrastructure. The Hood Canal Bridge (HCB) spans the northern outlet of Hood Canal in the Salish Sea, extends 4.6 m (15 ft) underwater, and forms a partial barrier for steelhead migrating from Hood Canal to the Pacific Ocean. Spatial mark–recapture survival models using acoustic telemetry data indicate that only 49% (2017; 95% confidence interval (CI) = 40%–58%) and 56% (2018; 95% CI = 48%–65%) of the steelhead smolts encountering the HCB survived past the bridge and 7 km to the next array. We studied fine‐scale movements of more than 300 steelhead smolts to understand how migration behavior was affected across the entire length of the HCB and to quantify spatial and temporal patterns of mortality. Individually coded acoustic telemetry transmitters implanted in juvenile steelhead were used in conjunction with an extensive array of acoustic receivers surrounding the HCB to obtain approximations of the path each steelhead took as they encountered the bridge structure. Steelhead survival past the HCB appeared unaffected by tidal stage, population‐of‐origin, approach location, current velocity, or time of day, but was influenced by week of bridge encounter. Behavioral data from transmitters with temperature and depth sensors ingested by predators are consistent with high levels of marine mammal predation. This study confirms the considerable impact of the HCB on Endangered Species Act‐listed steelhead smolt survival, and provides detailed information on the behavior of steelhead smolts and their predators at the HCB for use in planning recovery actions.
Plant–soil feedbacks help explain plant community productivity
Authors: Forero Leslie E. , Kulmatiski Andrew , Grenzer Josephine , Norton Jeanette 
Published: April 2022

AbstractPlant productivity often increases with species richness, but the mechanisms explaining this diversity–productivity relationship are not fully understood. We tested if plant–soil feedbacks (PSF) can help to explain how biomass production changes with species richness. Using a greenhouse experiment, we measured all 240 possible PSFs for 16 plant species. At the same time, 49 plant communities with diversities ranging from one to 16 species were grown in replicated pots. A suite of plant community growth models, parameterized with (PSF) or without PSF (Null) effects, was used to predict plant growth observed in the communities. Selection effects and complementarity effects in modeled and observed data were separated. Plants created soils that increased or decreased subsequent plant growth by 25% ± 10%, but because PSFs were negative for C3 and C4 grasses, neutral for forbs, and positive for legumes, the net effect of all PSFs was a 2% ± 17% decrease in plant growth. Experimental plant communities with 16 species produced 37% more biomass than monocultures due to complementarity. Null models incorrectly predicted that 16‐species communities would overyield due to selection effects. Adding PSF effects to Null models decreased selection effects, increased complementarity effects, and improved correlations between observed and predicted community biomass. PSF models predicted 26% of overyielding caused by complementarity observed in experimental communities. Relative to Null models, PSF models improved the predictions of the magnitude and mechanism of the diversity–productivity relationship. Results provide clear support for PSFs as one of several mechanisms that determine diversity–productivity relationships and help close the gap in understanding how biodiversity enhances ecosystem services such as biomass production.
Intestinal homeostasis and inflammation: Gut microbiota at the crossroads of pancreas–intestinal barrier axis
Authors: Zhang Zhongwei , Tanaka Izumi , Pan Zhen , Ernst Peter B , Kiyono Hiroshi , Kurashima Yosuke 
Published: April 2022

AbstractThe pancreas contains exocrine glands, which release enzymes (e.g., amylase, trypsin, and lipase) that are important for digestion and islets, which produce hormones. Digestive enzymes and hormones are secreted from the pancreas into the duodenum and bloodstream, respectively. Growing evidence suggests that the roles of the pancreas extend to not only the secretion of digestive enzymes and hormones but also to the regulation of intestinal homeostasis and inflammation (e.g., mucosal defense to pathogens and pathobionts). Organ crosstalk between the pancreas and intestine is linked to a range of physiological, immunological, and pathological activities, such as the regulation of the gut microbiota by the pancreatic proteins and lipids, the retroaction of the gut microbiota on the pancreas, the relationship between inflammatory bowel disease, and pancreatic diseases. We herein discuss the current understanding of the pancreas–intestinal barrier axis and the control of commensal bacteria in intestinal inflammation.
Multiple sclerosis: Immunopathological heterogeneity and its implications
Authors: Engelhardt Britta , Comabella Manuel , Chan Andrew 
Published: April 2022

AbstractMS is the most common autoimmune demyelinating disease of the CNS. For the past decades, several immunomodulatory disease‐modifying treatments with multiple presumed mechanisms of action have been developed, but MS remains an incurable disease. Whereas high efficacy, at least in early disease, corroborates underlying immunopathophysiology, there is profound heterogeneity in clinical presentation as well as immunophenotypes that may also vary over time. In addition, functional plasticity in the immune system as well as in the inflamed CNS further contributes to disease heterogeneity. In this review, we will highlight immune‐pathophysiological and associated clinical heterogeneity that may have an implication for more precise immunomodulatory therapeutic strategies in MS.
Comparing randomized controlled trials of outpatient family‐based or inpatient multimodal treatment followed by outpatient care in youth with anorexia nervosa: Differences in populations, metrics, and outcomes
Authors: Haas Verena , Nadler Janine , Crosby Ross D. , Madden Sloane , Kohn Michael , Le Grange Daniel , Gonçalves Ana Sofia Oliveira , Hebebrand Johannes , Correll Christoph U. 
Published: April 2022

AbstractObjectiveVarious approaches exist to treat youth with anorexia nervosa (AN). Family‐based treatment (FBT) has never been compared to long inpatient, multimodal treatment (IMT) in a randomized controlled trial (RCT). The aim of this study was to compare data on body weight trajectories, change in eating disorder psychopathology, hospital days and treatment costs in RCTs delivering FBT or IMT.MethodReview of RCTs published between 2010 and 2020 in youth with AN, delivering FBT or IMT.ResultsFour RCTs delivering FBT (United States, n = 2; Australia, n = 2), one RCT delivering Family Therapy for AN (United Kingdom) and two RCTs delivering IMT (France, n = 1; Germany, n = 1) were identified from previous meta‐analyses. The comparison of studies was limited by (1) significant differences in patient baseline characteristics including pretreated versus non‐pretreated patients, (2) use of different psychometric and weight measures and (3) different initial velocity of weight recovery. Minimal baseline and outcome reporting standards for body weight metrics and nature/dose of interventions allowing international comparison are needed and suggestions to developing these standards are presented.DiscussionAn RCT should investigate, whether FBT is a viable alternative to IMT, leading to comparable weight and psychopathology improvement with less inpatient time and costs.
Staff perceptions of the consequences of COVID‐19 on quality of dementia care for residents in Ontario long‐term care homes
Authors: Kirkham Julia , Shorey Carrie L. , Iaboni Andrea , Quirt Hannah , Grigorovich Alisa , Astell Arlene , Lin Esther , Maxwell Colleen J. 
Published: April 2022

AbstractObjectivesThe first wave of the COVID‐19 pandemic necessitated extensive infection control measures in long‐term care (LTC) and had a significant impact on staffing and services. Anecdotal reports indicate that this negatively affected LTC residents' quality of care and wellbeing, but there is scarce evidence on the effects of COVID‐19 on quality of dementia care in LTC.MethodsFrom December 2020 to March 2021, we conducted a cross‐sectional online survey among staff who worked in LTC homes in Ontario, Canada. Survey questions examined staffs' perceptions of the impact of COVID‐19 on dementia quality of care during the initial wave of the COVID‐19 pandemic (beginning 1 March 2020).ResultsThere were a total of 227 survey respondents; more than half reported both worsened overall quality of care (51.3%) and worsening of a majority of specific quality of care measures (55.5%). Measures of cognitive functioning, mobility and behavioural symptoms were most frequently described as worsened. Medical and allied/support staff had the highest odds of reporting overall worsened quality of care, while specialized behavioural care staff and those with more experience in LTC were less likely to. LTC home factors including rural location and smaller size, staffing challenges, higher number of outbreaks and less COVID‐19 preparedness were associated with increased odds of perceived worsening of quality of dementia care outcomes.ConclusionsThese findings suggest that COVID‐19 pandemic restrictions and related effects such as inadequate staffing may have contributed to poor quality of care and outcomes for those with dementia in LTC.
New Findings on Unconsented Intimate Exams Suggest Racial Bias and Gender Parity
Authors: Bruce Lori , Hannikainen Ivar R. , Earp Brian D. 
Published: April 2022

AbstractTestimony from hundreds of medical students and numerous physicians and scholars suggests that unconsented intimate exams (UIEs) are unlikely to be rare, isolated incidents. However, much is unknown about the frequency of these exams and the circumstances in which they take place. The Community Bioethics Forum, founded and chaired by one of the authors of this commentary, is a consultative group of diverse community members who provide insights on law and policy to policy‐makers and medical associations. Connecticut legislators asked the CBF to provide their views on proposed “explicit consent” legislation, and during those discussions, concerning narratives emerged about members’ (and their loved ones’) personal experiences with UIEs.To gain greater clarity on the demographic patterns and frequency of UIEs, we conducted the first national survey on UIEs. Data from this survey suggest that UIEs may occur under a broader range of circumstances than addressed by most law and policy. The survey resulted in nearly the exact same rate of affirmative responses between males and females in answer to whether they had received a UIE within the past five years. The survey results also showed evidence of racial disparity. Additional research is needed to understand the nature of UIEs.
BeforeThe Birth of Bioethics: James M. Gustafson at Yale
Authors: Shulman Kaiulani S. , Fins Joseph J. 
Published: April 2022

AbstractIn the 1960s, tucked away at Yale Divinity School, there was a remarkable confluence of bioethics scholars under the tutelage of James M. Gustafson. His students were Jim Childress, Albert R. Jonsen, Tom Beauchamp, LeRoy Walters, Jim Drane, and Stanley Hauerwas, among others. Jonsen later recalled, “That little group was really the beginning of scholarly bioethics.” Yet despite Gustafson’s influence on the founding generation of bioethics scholars and his prominence as a leading mid‐century Christian theologian, his legacy in bioethics is unsecured. This is an unfortunate omission, as Gustafson’s contributions to bioethics were not limited to the classroom. In 1969, he brought Paul Ramsey to Yale to deliver the Lyman Beecher Lectures, a collection of talks that would coalesce into Ramsey’sThe Patient as Person.Gustafson also advised Daniel Callahan as Callahan and Willard Gaylin founded The Hastings Center, on whose founding board of directors Gustafson later served. Through archival research and interviews with Gustafson’s former students and colleagues, we recount his biography, consider his pedagogy, and examine the theological pragmatism that informed his engagement with his students and his intellectual commitments before the birth of bioethics. By reviewing Gustafson’s contributions to the nascent field of bioethics, we seek to understand why his legacy has been forgotten and to introduce him to a new generation of bioethics scholars.
Industry Technicians Embedded in Clinical Teams: Impacts on Medical Knowledge
Authors: Howard Mark , Hutchinson Katrina 
Published: April 2022

AbstractAdvances in implantable medical devices have increased the role for industry‐employed allied professionals (IEAPs) in providing training and support during surgical procedures and follow‐up care. The effect of these changes on the organization of medical knowledge and the sharing of information remains largely unexplored. Recent work in social epistemology and the conceptualizing of implantable medical device companies as part of a knowledge‐based industry provide a framework for engaging with this issue. In this article, we argue that the insertion of industry technicians into hospitals diminishes epistemically valuable knowledge‐sharing practices. This is in part a result of health care professionals’ increased dependence on IEAPs, who control access to knowledge about devices, limiting opportunities both for independent learning and for dialogic education practices through which participants work toward a common goal. Ultimately, we claim that overdependence on the IEAP as expert undermines a basic social value regarding knowledge sharing for everyone’s benefit.
Representative‐negotiated i‐deals for people with disabilities
Authors: Ho Jennifer A. , Bonaccio Silvia , Connelly Catherine E. , Gellatly Ian R. 
Published: April 2022

AbstractAlthough substantial research has been devoted to describing the challenges people with disabilities face in the workplace, much less attention has been focused on the processes that can bring about change. This article explores a proactive process, representative‐negotiated idiosyncratic work arrangements (i‐deals), that can create the conditions for long‐term employment for people with disabilities. Specifically, we explored the factors associated with the development and success of representative‐negotiated i‐deals for people with disabilities. Using focus groups and interviews with employers and job developers, we identified nine factors and two prevailing conditions that explain the contexts in which representative‐negotiated i‐deals will be successful. In doing so, we identified the negotiation stage during which these factors and prevailing conditions influence the i‐deals negotiation process. Representative‐negotiated i‐deals offer insights into how employees with disabilities can find more meaningful work. Together, these findings underscore how the representative i‐deals negotiation process is only viable if the facilitating factors are present and supported, while the absence of these factors can hinder and lead to failure.
Metabolic Switch Under Glucose Deprivation Leading to Discovery of NR2F1 as a Stimulus of Osteoblast Differentiation
Authors: Lee Eugene , Park Seo-Young , Moon Jae-Yeon , Ko Ji-Yun , Kim Tae Kyung , Im Gun-Il 
Published: April 2022

ABSTRACT
Poor survival of grafted cells is the major impediment of successful cell-based therapies for bone regeneration. Implanted cells undergo rapid death in an ischemic environment largely because of hypoxia and metabolic stress from glucose deficiency. Understanding the intracellular metabolic processes and finding genes that can improve cell survival in these inhospitable conditions are necessary to enhance the success of cell therapies. Thus, the purpose of this study was to investigate changes of metabolic profile in glucose-deprived human bone marrow stromal/stem cells (hBMSCs) through metabolomics analysis and discover genes that could promote cell survival and osteogenic differentiation in a glucose-deprived microenvironment. Metabolomics analysis was performed to determine metabolic changes in a glucose stress metabolic model. In the absence of glucose, expression levels of all metabolites involved in glycolysis were significantly decreased than those in a glucose-supplemented state. In glucose-deprived osteogenic differentiation, reliance on tricarboxylic acid cycle (TCA)-predicted oxidative phosphorylation instead of glycolysis as the main mechanism for energy production in osteogenic induction. By comparing differentially expressed genes between glucose-deprived and glucose-supplemented hBMSCs, NR2F1 (Nuclear Receptor Subfamily 2 Group F Member 1) gene was discovered to be associated with enhanced survival and osteogenic differentiation in cells under metabolic stress. Small, interfering RNA (siRNA) for NR2F1 reduced cell viability and osteogenic differentiation of hBMSCs under glucose-supplemented conditions whereas NR2F1 overexpression enhanced osteogenic differentiation and cell survival of hBMSCs in glucose-deprived osteogenic conditions via the protein kinase B (AKT)/extracellular signal-regulated kinase (ERK) pathway. NR2F1-transfected hBMSCs significantly enhanced new bone formation in a critical size long-bone defect of rats compared with control vector-transfected hBMSCs. In conclusion, the results of this study provide an understanding of the metabolic profile of implanted cells in an ischemic microenvironment and demonstrate that NR2F1 treatment may overcome this deprivation by enhancing AKT and ERK regulation. These findings can be utilized in regenerative medicine for bone regeneration. © 2022 American Society for Bone and Mineral Research (ASBMR).
Ultrasonic multimodality imaging features and the classification value of nonpuerperal mastitis
Authors: Li Chunxiao , Yao Minghua , Li Xin , Shao Sihui , Chen Jing , Li Gang , Jia Chao , Wu Rong 
Published: April 2022

AbstractObjectiveTo explore the value of ultrasonic multimodality imaging for characterizing nonpuerperal mastitis (NPM) lesions and feasibility of distinguishing different subtypes.MethodsThirty‐eight NPM lesions were assessed using conventional ultrasonography (US), strain elastography (SE), and contrast‐enhanced ultrasound (CEUS). The lesions were confirmed pathologically and classified as granulomatous lobular mastitis (GLM), plasma cell mastitis (PCM), or nonspecific mastitis (NSM). Furthermore, diagnostic indicators were evaluated. The diagnostic performances of the modalities were compared using the area under the receiver operating characteristic curve (AUC).ResultsThe overall morphological features on US differed significantly between the GLM and PCM groups (p = 0.002). Lesion size (≤10 mm) (p = 0.003) and mean SE score (p = 0.001) differed significantly between the PCM and NSM groups. The frequent NPM characteristic on CEUS was hyperenhancement with (or without) increased lesion size; intergroup differences were not significant. Breast Imaging Reporting and Data System > 3 was considered to indicate malignancy; accordingly, the accuracy of US alone, US with CEUS, and US with SE was 10.5%, 21.1%, and 65.8%, respectively. Moreover, the AUC for US with SE for classifying GLM and PCM was 0.616.ConclusionCEUS cannot accurately classify NPM subtypes, while US and SE are valuable for classification.
Light and scanning electron microscopy observations on morphological diversity of Rosaceae stigma and its taxonomic significance
Authors: Ren Simin , Lu Xiaojian , Hu Ziming , Liu Jiaxi 
Published: April 2022

AbstractThe stigma morphology can provide a reference for exploring plant systematics and pollination biology. In this study, we observed the stigma morphological characteristics of Rosaceae in Beijing urban area in detail using light microscopy and scanning electron microscopy. The stigma of Rosaceae is entire or bilobed and mostly baculate, crateriform, cristate, discoid, or flattened. The stigma surface may have irregular, strongly raised ridges; or flat without papillae; or composed of densely or loosely arranged papillary cells. Surface ornamentation includes fossulate, psilate, psilate‐striate, rugulate, scabrate, striate, and striate‐rugulate. There are similarities in stigma morphology among genera and differences in stigma morphology among species within genera. The stigma shape supports the view of molecular systematic classification, that is, the former subfamilies Maloideae, Prunoideae, and Spiraeoideae are grouped into subfamily Amygdaloideae.Research Highlights
Scanning electron microscopy (SEM) was used to provide high‐quality figures for observing stigma morphology.
The data on the morphological diversity of stigma were provided to further explore the systematics and pollination biology of Rosaceae.