bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tanshione IIA reduces astrocyte swelling after oxygen-glucose deprivation and reoxygenation by inhibition of the HMGB1/RAGE/NF-κB/IL-6 pro-inflammatory axis and AQP4 over-clustering in the plasma membrane
Authors: Tang Zhaohua , Yang Gang , Liao Zhengbu , Chen Feilan , Chen Song , Liu Mengqi , Huo Gang , Sun Xiaochuan , Wang Xiaoshu 
Published: April 2022

Abstract
Background: Tanshione IIA (TSO IIA) has emerged as a powerful anti-inflammatory compound with multiple therapeutic properties. Here, we investigated the role of TSO IIA in astrocytic swelling caused by ischemia and reperfusion-like injury in an in vitro model and the molecular mechanisms underlying this effect. Methods: Primary brain astrocytes were cultured in vitro in conditions of glucose and oxygen deprivation and reoxygenation (OGD/R). We investigated the effects of TSO IIA treatment on cell swelling and injury, and the expression levels of AQP4 protein in the plasma membrane. We then studied the involvement of the HMGB1/RAGE/NF-κB/IL-6 pro-inflammatory axis in TSO IIA-mediated protection. Results: Treatment with TSO IIA alleviated OGD/R-induced astrocytic swelling, changes in cell morphology and ultrastructure, the release of LDH, and the over-clustering of AQP4 protein in the plasma membrane. In addition, TSO-IIA significantly reduced over-expression of HMGB1 protein in the cytoplasm and surrounding medium, the high levels of NF-κB protein in the nucleus, and of IL-6 protein in cytoplasm and extracellular media induced by OGD/R. Combination of TSO IIA and recombinant HMGB1 (rHMGB1) reversed these effects. Inhibition of RAGE, the receptor of HMGB1, via administration of FPS-ZM1 (a RAGE antagonist) induced similar results to those of TSO IIA. The combination of HMGB1 and FPS-ZM1 did not alter the effects mediated by FPS-ZM1 alone. In addition, exogenous IL-6 reversed TSO IIA-mediated effect on AQP4 protein clustering in the plasma membrane, cell swelling and LDH activity.Conclusions: TSO IIA significantly reduced astrocyte swelling after OGD/R injury in vitro, via blocking activation of the HMGB1/RAGE/NF-κB/IL-6 pro-inflammatory axis and thereby decreasing the expression of AQP4 in plasma membrane.
Neonatologist Performed Bedside Ultrasound for Early Diagnosis of Necrotizing Enterocolitis: A Pilot Before-after Study
Authors: Oulego-Erroz Ignacio , Rodríguez-Fanjul Javier , Terroba-Seara Sandra , Sorribes-Ortí Clara , Fernández-Fernández David , Fuentes-Martínez Silvia , Pou-Blázquez Alvaro 
Published: April 2022

Abstract
Purpose
To assess whether bedside ultrasound (BUS) improves early diagnosis of necrotizing enterocolitis (NEC).
Methods
Before-after controlled study in two NICUs. Infants below 37 weeks of gestational age with suspected NEC were eligible. The intervention group (October 2019-October 2021) received BUS as first imaging modality and treatment was guided by BUS findings. The control group (October 2015-September 2019) received radiography as first imaging modality and was managed accordingly. The main outcome was NEC confirmation at the time of initial imaging. Secondary outcomes included radiographic and laboratory data, treatment requirements, surgical NEC and NEC-related death:
Results
Thirty-five episodes of suspected NEC with 14 (40%) confirmed NEC cases and 49 episodes of suspected NEC with 22 (44.9%) confirmed NEC cases were included in the intervention and control group respectively. In the intervention group 14 of 14 (100%) NEC cases were confirmed at initial evaluation compared to 10 of 22 (45.4%) in the control group (p = 0.001). Infants managed with BUS developed thrombocytopenia and coagulopathy less frequently, were exposed to less radiation, progressed less frequently to Bell’s stage ≥ II and required less days of parenteral nutrition compared to the control group (p < 0.05). Surgical NEC [4 of 14 (28.6%) vs 8 of 22 (36.7%); p = 0.727] and NEC-related death [0 of 14 (0%) vs 5 of 22 (22.7%); p = 0.133] were not different.
Conclusions
The use of BUS as first imaging modality resulted in an earlier diagnosis of NEC compared to abdominal radiography. Further research should determine the potential of BUS to improve outcomes.
Intrinsic brain connectivity differentiates intact endogenous pain modulation in subjects with neuropathic pain after spinal cord injury
Authors: Huynh Vincent , Lütolf Robin , Rosner Jan , Luechinger Roger , Curt Armin , Kollias Spyridon , Michels Lars , Hubli Michèle 
Published: April 2022

Abstract
Endogenous pain modulation in humans is frequently investigated with conditioned pain modulation (CPM) and deficient pain inhibition is a proposed mechanism that contributes to neuropathic pain (NP) after spinal cord injury (SCI). Recent studies have combined CPM testing and neuroimaging to reveal neural correlates of CPM efficiency in chronic pain. This study investigated the unexplored differences in CPM efficiency in relation to resting-state functional connectivity (rsFC) between 13 SCI-NP subjects and 13 age- and sex-matched healthy controls (HC). Psychophysical readouts were analyzed to determine CPM efficiency within and between cohorts. To explore group differences of rsFC in relation to CPM efficiency, seed-to-voxel rsFC analyses were performed within pain modulatory regions, e.g., ventrolateral periaqueductal gray (vlPAG) and amygdala. Overall, pain inhibition was not deficient in SCI-NP subjects and was greater in those with more intense NP. In addition, in SCI-NP greater pain inhibition was associated with weaker rsFC between the vlPAG and amygdala with the visual and frontal cortex, respectively, but with stronger rsFC in HC. Taken together, SCI-NP subjects present with intact pain inhibition, but can be differentiated from HC by an inverse relationship between CPM efficiency and intrinsic connectivity of supraspinal regions. Specifically, abnormal neural plasticity could be related to weaker pain modulatory function in SCI-related NP.
Heavy Metals Research in Nigeria: A Review of Studies and Prioritization of Research needs
Authors: Idowu Gideon 
Published: April 2022

Abstract
Nigeria is experiencing continuous economic and industrial transformations, typical of many developing nations. In addition to its well-established oil industry, which is infamous for exuding various kinds of pollutants, there is increased mining operations, indiscriminate disposal and burning of wastes, illegal oil refinery, and terroristic insurgency, all poised to increase the levels of heavy metal contaminants in the Nigerian environment. A recent revelation indicates that about 2 million people in Southwestern Nigeria alone could potentially be poisoned by lead (Pb) and mercury (Hg), emanating from illegal mining operations. This further underscores the importance of investigations of toxic trace metal levels in the country. The current review of 136 research articles was conducted to provide an understanding of the scope of heavy metals research in Nigeria and to prioritise needed research. The review recognised that the scope of heavy metals studies has been wide, covering matrices such as cosmetics, human blood, hair, medicines, foods, beverages, water, air, soil and crude-oil. However, important toxic metals, especially mercury (Hg), arsenic (As) and antimony (Sb) are largely under-investigated. Also, there is a need for more studies to be conducted in the Northern part of the country. Furthermore, studies need to focus on marine environments rather than the freshwater ecosystems alone. Techniques such as the inductively coupled plasma - optical emission spectrometry (ICP-OES) and particle - induced X-ray emission (PIXE) analyses are herein recommended to bridge the data gap, and to overcome limitations in trace metals analyses in the Nigerian total environment.
Region-dependent meteorological conditions for the winter cold hazards with and without precipitation in China
Authors: Yu Yueyue , Wenwen Yang , Lingli Zhang , Zhaoyong Guan , Qinlan Yang , Muxin Hu , Wentian Qiu , Jingyi Wang 
Published: April 2022

Abstract
Cold hazard is one of the major meteorological disasters in winter. However, the meteorological conditions for the cold hazard events vary significantly with both the feature of the event and the region of occurrence. This study divided winter cold hazard events in China into three categories based on the daily gridded dataset of cold hazards from November 1980 to March 2020: events without wintry precipitation (hazardous low temperature, abrupt temperature drop, and/or freezing), with wintry precipitation only (hazardous sleet and/or snowstorm), and with both. The region-dependent multivariate meteorological conditions for each category of cold hazards are investigated using ERA5 reanalysis data. Results show that the surface air temperature (T2m) and its anomaly (T2m_anom) are lower than climatology during cold hazards. But the difference in T2m among provinces exceeds 30°C, and even for the same province, the difference among different categories of cold hazards exceeds 10°C. The region- and category-dependent differences of T2m_anom and daily temperature drop (∆T24) are also large, about 5°C and 2°C d-1, respectively. The Multivariate Empirical Orthogonal Function analysis has further been applied to not only the abovementioned temperature-related variables but also the precipitation-related variables (i.e., daily accumulated total precipitation, daily accumulated snowfall, and daily mean snow depth) in the middle and lower Yangtze River region, which reveals the event-mean state and spatial-temporal coupling evolution during the progression of the event for the selected key meteorological variables. The meteorological conditions for cold hazards put forward by this study could provide region-dependent and category-dependent reference for the prediction and warning of cold hazards.
Quantitative Analysis of Merkel Cell Polyomavirus (MCPyV) Genome in Non-Melanoma Skin Cancer and Normal Tumor Margins
Authors: Hasanzadeh Ali , Sadeghi Farzin , Kamrani Ghodsieh , Mohammadi Neda , Vosough Zeinab , Ranaee Mohammad , Tabarraei Alijan , Yahyapour Yousef 
Published: April 2022

Abstract
Merkel cell polyomavirus (MCPyV) is the cause of approximately 80% of Merkel cell carcinomas (MCC). The common types of Non-Melanoma skin cancer (NMSC) including squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) are histologically similar to MCC. In the present study, 58 NMSC formalin-fixed paraffin-embedded tissue (FFPE) samples including 12 SCC, 46 BCC and 58 FFPE samples of adjacent non-tumoral margins as the control were included. Determination of large Tumor antigens (LTAg) copy number in samples was performed by qReal Time PCR as a viral copy number per cell. Out of 58 samples, 36(62%) cancerous and 22(37.9%) normal tumor margins were positive for MCPyV LTAg. Median copy numbers of MCPyV LTAg among all NMSC samples and non-tumoral margins were 0.308×10-2 and 0.269×10-3 copies per cell respectively (P=0.001). In addition, although the viral load in the majority of samples was detected to belower than one copy per cell, in 4 BCC samples, a viral load higher than one LTAg copy per cell was detected. The present study revealed that the detection of higher levels of MCPyV LTAg viral load in some BCC and SCC samples may be correlated with the role of MCPyV in some cases of BCC and SCC skin cancer.
Influence of monsoon low pressure systems on South Asian disasters and implications for disaster prediction
Authors: Suhas D. L. , Ramesh Nandini , Kripa Ram M. , Boos William R. 
Published: April 2022

Abstract
Transient atmospheric vortices called monsoon low pressure systems (LPS) generate a large fraction of total rainfall over South Asia and often produce extreme precipitation. Here, we assess the influence of these storms on the occurrence of disasters, using information from the Emergency Events Database (EM-DAT) that we geocoded and then associated with LPS tracks. We show that more than half of hydro-meteorological disasters over South Asia during summer can be attributed to these LPS events. Weaker LPS (which are called monsoon lows) occur more frequently than stronger LPS (called monsoon depressions), but the stronger LPS produce a larger number of disasters. Furthermore, although many prior studies have shown that the peak rainfall in LPS falls southwest of the vortex center, the disasters are concentrated on the northern edge of the LPS tracks, along the Himalayas and upper basins of the Ganga and Brahmaputra rivers. Observations show a sharp peak in rainfall on the day of disasters, confirming the causal physical link between LPS and these hydro-meteorological disasters. A similar peak in rainfall is found in weather forecasts made up to five days before the disaster, suggesting that short-term forecasts of LPS tracks and LPS precipitation can be useful in disaster preparation.
COVID-19 impact on the psychological health of Latinx transgender and non-binary individuals in mainland United States and Puerto Rico: A mixed-methods study.
Authors: Ramos-Pibernus Alixida , Rodríguez-Madera Sheilla , Rosario-Hernández Ernesto , Moreta-Ávila Fabián , Silva-Reteguis Julián , Rivera-Segarra Eliut 
Published: April 2022

Abstract
Background
The COVID-19 pandemic continues to generate an unprecedented impact on all aspects of everyday life across the world. However, those with historically and currently marginalized identities (i.e., gender or ethnicity) who already experience a wide range of structural inequities have been disproportionally impacted. LTNB are a particularly at-risk population as they lie at the intersection of race/ethnicity, gender identity, language, migration status, geographical location, among others, which could further increase their COVID-19 and other health-related risks and disparities. The objective of this study was to examine the impact of key social determinants of health (i.e., gender identity, country, health insurance, employment) among a sample of LTNB individuals.
Methods
The team implemented a cross-sectional exploratory design with an online survey technique using the secure web platforms REDcap and SurveyMonkey. A total of 133 participants completed the online survey. Most of the sample self-identified as transwomen (38.8%), transmen (26.3%), and non-binary (21.8%) between the ages of 21 to 72. All participants were Latinx living in either Puerto Rico (47.7%) or mainland United States (52.3%). Descriptive statistics, reliability tests, Mann-Whitney and rapid thematic analysis test were conducted.
Results
Findings show that most participants were always (38.1%) or almost always (33.3%) worried about contracting COVID-19. Individuals living in Puerto Rico reported more difficulties than those residing in the mainland US regarding COVID-19 impact on psychosocial, emotional, and COVID-related thinking. Most participants' answers for the COVID-19 open-ended questions focused on three main domains: income, access to trans-affirmative health care, and coping strategies.
Discussion
Findings evidence that although most of LTNB participants were negatively impacted by the COVID-19 pandemic in multiple aspects of their lives, those living in Puerto Rico experienced these differently when compared to those in mainland US. More research is needed to understand better the mechanisms and pathways through which this context specifically impacts LTNB health and wellbeing, particularly in Puerto Rico. This study could help shape the public health response taking into account the geographical location and other intersectional identities that play critical roles in the production and reproduction of inequities.
Prognostic Genes of Sepsis-induced ARDS identified by Gene Co- expression Network Analysis
Authors: Zheng Yongxin , Qian Chuyun , Huang Yongbo , Xu Yonghao , Sang Ling , Liu Xiaoqing , Li Yimin 
Published: April 2022

Abstract
Background and objectives: Acute respiratory distress syndrome (ARDS) is a common and fatal complication of sepsis. The molecular mechanisms of early recognition are still limited. The aim of the study was to identify the potential different genes between sepsis alone and sepsis-induced ARDS. Methods: The gene expression profiles of GSE66890 were selected from the Gene Expression Omnibus (GEO) database for bioinformatic analysis. Weight gene co-expression network analysis (WGCNA) was used to construct a co-expression network to explore the associations between gene sets and clinical traits, and to identify the candidate hub genes. Then, GSE10474 and GSE32707 were used to validate our results. Results: A total of 11 modules were obtained via the average linkage hierarchical clustering. In the significant module, based on the |MM|＞0.9 and |GS|＞0.2, 4 genes TOP2A, CENPF, DLGAP5 and BIRC5 which had the highest connectivity were identified as the hub genes. Logistic regression model and receiver operating characteristic (ROC) curve validated that the expression levels of 4 hub genes exhibited a moderate diagnostic efficiency for sepsis alone and sepsis-induced ARDS. Among them, the 4 genes were significantly upregulated in sepsis-induced ARDS patients. Additionally, nomogram showed that 4 hub genes may have a good ability to predicting the probability of sepsis-induced ARDS patients. Conclusions: Our research provides a more effective understanding of the importance of biological pathways and the relationships between major genes in sepsis-induced ARDS in the perspective of searching for new molecular targets. TOP2A, CENPF, DLGAP5 and BIRC5 might be potential diagnostic biomarkers for sepsis-induced ARDS.
Genome-wide analysis of LBD transcription factors reveals PvLBD12 expression profile involved in salt stress in switchgrass (Panicum virgatum L.)
Authors: Guan Cong , Wu Bo , Zhang Jinhong , Ma Shu , Weng Hong , Wang Guoliang , Jia Chunlin 
Published: April 2022

Abstract
Abiotic stresses seriously limit plant development and productivity and harm the agricultural economy. It is important, therefore, to understand the mechanism of salt tolerance in plants. Lateral organ boundaries domain (LBD) proteins are plant-specific transcription factors that have been reported to regulate plant lateral organ development, plant regeneration, and abiotic stress. In this study, we identify 69 LBD family members from switchgrass genome-wide sequences, and classify them according to their homology with LBD genes in Arabidopsis. Sixty-two cis-acting elements were identified from switchgrass LBD genes, and we found that most cis-acting elements can respond to light, control plant growth and development, regulate circadian rhythm, and respond to stress. Similarly, the results of qRT-PCR showed that PvLBD genes had different expression patterns under abiotic stress conditions, indicating that they may play important roles in stress response. In addition, candidate PvLBD target genes were enriched in many GO and KEGG pathways that play vital roles in the whole growth process and environmental stress response. PvLBD12 was selected as a candidate gene for functional analysis, and overexpression of PvLBD12 enhanced salt tolerance of transgenic switchgrass by altering a wide range of physiological responses. Overall, this study provides valuable information for better understanding the potential biological functions of LBD genes in plants.
Postoperative Analgesia In Laparoscopic and Robotic Rectal Cancer Surgeries Compared to Laparotomy approach: A Retrospective Analysis
Authors: WANG Xiao , ELMAWIEH Jamie , SITBON Philippe , Jean-Louis Bourgain 
Published: April 2022

Abstract
Purpose: Although epidural analgesia (EA) is recommended for laparotomic (LT) rectal surgery, there is no consensus regarding pain management in laparoscopic or robotic (LR) rectal cancer surgery. According to our local guidelines, EA is usually chosen for LT rectal procedures and intravenous analgesia for LR rectal procedures. Methods: This retrospective study included patients who underwent rectal cancer surgery in our center from January 2016 to February 2020 using either laparotomy (LT) or laparoscopic or robotic (LR) techniques. Analgesia technique varied according to surgery technique, the choice of patient and anesthesia provider. Data were acquired from electronic databases and consisted of pain scores in the recovery room and the three postoperative days, morphine consumption and total length of hospital stay. Results: 151 patients were included: 92 in the LR group and 59 in the LT group. Epidural analgesia was used for 8/92 LR patients and of 48/59 LT patients. Pain scores were comparable regardless of the surgical and analgesic technique in the three postoperative days, but patients with epidural analgesia used significantly less morphine, even in the LR group. After correction for multiple pairwise comparisons, our results showed that in laparoscopic rectal surgery, the use of epidural analgesia was associated with significantly lower morphine consumption (corrected p =0.01, reduction over 3 days: 25 mg). Conclusion: Compared to other analgesia regimens, perioperative epidural analgesia for LR rectal surgery was associated with significantly less postoperative morphine consumption to achieve the same pain scores. EA appears to be beneficial even in laparoscopic rectal surgery.
Molecular docking analysis of chemical constituents from the stem barks of podocarpus falcatus and evaluation for antibacterial activity
Authors: Deresa Desalegn Abebe , Abdissa Zelalem , Gurmessa Getahun Tadesse. , Abdissa Negera 
Published: April 2022

Abstract
Chromatographic separation of equal ratio of CH2Cl2-MeOH extract of the stem barks of Podocarpus falcatus led to the isolation of three compounds namely: 4β-carboxy-19-nor-totarol (1), β-sitosterol (2), 4-hydroxybenzoic acid (3) and (E)-methyl-3, 4, 5-trimethylhex-2-enoate (4). The structures of the compounds were established based on the analysis of 1D and 2D NMR spectroscopic data. These compounds were reported from this plant for the first time. The crude extract and isolated compounds were evaluated for their antibacterial activity using disk diffusion assay method. The crude extract showed a strong activity against S. aureus. Compounds (1) and (2) showed a relatively moderate activity against S. flexineri and S. typhimurium respectively, whereas, compound (3) and (4) demonstrated a strong activity against S. aureus. The crude extract and the isolated compounds showed considerable antibacterial activity as compared to the reference gentamycin indicating that this plant has potentially antibacterial properties. Docking studies were performed with S. aureus Gyrase and human DNA topoisomerase IIβ by using AutoDock Vina. ADMET were predicted by Swiss ADME, Pre ADMET. In silico, molecular docking studies of compounds (1-3) showed strong interaction with S. aureus Gyrase with binding energy value ranging from −6.1 to −8.6 kcal/mol, with respect to ciprofloxacin and Doxycycline −8.4 kcal/mol and -13.0 kcal/mol respectively, whereas, analysis against human topoisomerase IIβ with binding energy value ranging from −6.4 to −10.1 kcal/mol, with respect to Vosaroxin and Abiraterone −10.2 and -11.8 kcal/mol respectively. The results obtained suggest that compounds (1-3) are potential S. aureus Gyrase inhibitors and might be used as antibacterial agents and compound (1 and 2) are potential topoisomerase IIβ inhibitors and might be used as anticancer agents. The ADMET studies showed the highest drug-likeness properties for studied compounds (1-3). Compounds (1-3) have no acute toxicity. The studied compounds were predicted to be non- hepatotoxic, non- cytotoxic, non-mutagenic and non-irritant. However, compound (2) is Immunotoxic