bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pan-cancer analysis of microRNA expression profiles highlights microRNAs enriched in normal body cells as effective suppressors of multiple tumor types: A study based on TCGA database
Authors: Moradi Sharif , Kamal Aryan , Aboulkheyr Es Hamidreza , Farhadi Farnoosh , Ebrahimi Marzieh , Chitsaz Hamidreza , Sharifi-Zarchi Ali , Baharvand Hossein 
Published: April 2022

Background
MicroRNAs (miRNAs) are frequently deregulated in various types of cancer. While antisense oligonucleotides are used to block oncomiRs, delivery of tumour-suppressive miRNAs holds great potential as a potent anti-cancer strategy. Here, we aim to determine, and functionally analyse, miRNAs that are lowly expressed in various types of tumour but abundantly expressed in multiple normal tissues.

Methods
The miRNA sequencing data of 14 cancer types were downloaded from the TCGA dataset. Significant differences in miRNA expression between tumor and normal samples were calculated using limma package (R programming). An adjusted p value < 0.05 was used to compare normal versus tumor miRNA expression profiles. The predicted gene targets were obtained using TargetScan, miRanda, and miRDB and then subjected to gene ontology analysis using Enrichr. Only GO terms with an adjusted p < 0.05 were considered statistically significant. All data from wet-lab experiments (cell viability assays and flow cytometry) were expressed as means ± SEM, and their differences were analyzed using GraphPad Prism software (Student’s t test, p < 0.05).

Results
By compiling all publicly available miRNA profiling data from The Cancer Genome Atlas (TCGA) Pan-Cancer Project, we reveal a small set of tumour-suppressing miRNAs (which we designate as ’normomiRs’) that are highly expressed in 14 types of normal tissues but poorly expressed in corresponding tumour tissues. Interestingly, muscle-enriched miRNAs (e.g. miR-133a/b and miR-206) and miRNAs from DLK1-DIO3 locus (e.g. miR-381 and miR-411) constitute a large fraction of the normomiRs. Moreover, we define that the CCCGU motif is absent in the oncomiRs’ seed sequences but present in a fraction of tumour-suppressive miRNAs. Finally, the gain of function of candidate normomiRs across several cancer cell types indicates that miR-206 and miR-381 exert the most potent inhibition on multiple cancer types in vitro.

Conclusion
Our results reveal a pan-cancer set of tumour-suppressing miRNAs and highlight the potential of miRNA-replacement therapies for targeting multiple types of tumour.
Effects of oral glutamine supplementation on jejunal morphology, development, and amino acid profiles in male low birth weight suckling piglets
Authors: Schregel Johannes , Schulze Holthausen Johannes , Sciascia Quentin L. , Li Zeyang , Görs Solvig , Eggert Anja , Tuchscherer Armin , Zentek Jürgen , Metges Cornelia C. 
Published: April 2022

BackgroundIt has been shown that small intestine development in low birth weight(LBW)piglets is impaired. Glutamine(Gln)has been reported to improve piglet health and intestinal function in weaned piglets, but data is scarce in suckling piglets. This study was conducted to investigate the effects of oral Gln supplementation compared to Alanine (Ala) on jejunal development and function in 5 and 12 d old male LBW and normal birth weight(NBW)suckling piglets.ResultsGln had no effect on the jejunal morphology, development, tissue and digesta amino acid profiles and mRNA abundance of genes involved in amino acid transport, metabolism, glutathione synthesis in LBW piglets when compared to Ala supplementation and birth weight controls at 5 and 12 d. Only the concentration of Gln in jejunal tissue was higher in NBW piglets supplemented with Gln compared to Ala at 5 d (P < 0.05). A comparison of the birth weight groups showed no differences between LBW and NBW piglets at 5 and 12 d in any parameter. Jejunal crypt depth, villus height / width, tunica muscularis thickness, number of goblet and IgA positive cells, the ratio of jejunal RNA to DNA and the concentration of DNA, protein and RNA changed (P < 0.05) from 5 compared to 12 d. The concentrations of several free, and protein bound amino acids as well as amino metabolites differed between age groups in jejunal tissue but the digesta concentrations were affected to a lesser extent.ConclusionsOral Gln supplementation to suckling male piglets over the first 12 d of life was not associated with changes in jejunal parameters measured in this study. The absence of effects may indicate that Gln is absorbed as well as metabolized in the upper intestinal tract and thus could benefit intestinal development at a more proximal location.
A qualitative study on community use of antibiotics in Kinshasa, Democratic Republic of Congo
Authors: Shembo Aurélie Koho Pungu , Musumari Patou Masika , Srithanaviboonchai Kriengkrai , Tangmunkongvorakul Arunrat , Dalleur Olivia 
Published: April 2022

Background
Resistance to antibiotics is an increasing and major threat to global health. While the large majority of antimicrobial use occurs in the community where antibiotics are available without prescription, we did not find any studies investigating community-level factors influencing the inappropriate use of antibiotics in the Democratic Republic of Congo (DRC), where non-prescription antibiotic use is prevalent.

Methods
This qualitative study was conducted from April 1st 2019 to May 5th 2019 and consisting of in-depth semi-structured interviews, utilizing purposive and snowball sampling schemes to recruit adult heads of households in the Pakadjuma slum, in Kinshasa, DRC. Participants with differing medical and educational backgrounds were selected. We employed a thematic analysis approach to explore community knowledge and use of antibiotics in the sampled population.

Results
A total of 18 participants with a median age of 35 years were interviewed. The majority was female (77.7%), had at least a secondary education (83.4%), and unemployed (61.1%). We found that participants were familiar with the term “antibiotics”, but had limited knowledge of the indications and risks of antibiotics, including the risk of antibiotic resistance. Inappropriate use of antibiotics was common and there was frequent self-medication of non-prescribed medicines for a range of non-indicated conditions such as menstruation. Having limited income was the most commonly reported reason for not visiting a health facility for appropriate health care.

Conclusion
Inappropriate use of antibiotics is a widespread practice and is influenced by lack of adequate knowledge of antibiotic use, indications and risks, prevalent self-medication, and financial barriers to accessing appropriate health care. There is need for both community education as well as structural interventions addressing poverty in order to reduce the inappropriate use of antibiotics in the Pakadjuma slum in Kinshasa.
Limited hyperoxia-induced proliferative retinopathy: A model of persistent retinal vascular dysfunction, preretinal fibrosis and hyaloidal vascular reprogramming for retinal rescue
Authors: Tedeschi Thomas , Lee Kendal , Zhu Wei , Fawzi Amani A. 
Published: April 2022

Background
Retinopathy of prematurity (ROP) remains the leading cause for blindness in children. Limited hyperoxia induced proliferative retinopathy (L-HIPR) was recently introduced as a potential animal model for ROP and persistent fetal vasculature; however, the detailed pathological changes remain unclear.

Methods
To model L-HIPR, we placed C57BL/6J mice in 65% oxygen from birth to post-natal day 7 (P7). We examined eyes at intervals between P12 and P30. Retinal morphometry, thickness, and preretinal fibrosis were quantified at different time points on histological sections stained with hematoxylin and eosin (H&E) and Masson Trichrome, respectively. Vascular development, angiogenesis, inflammation, and pericyte coverage were analyzed using immunohistochemistry staining in retinal flat mounts and cross sections.

Results
In L-HIPR, the hyaloidal vessels persisted until the latest time point in this study, P30 and began to invaginate the peripheral then central retina starting at P12. Central retinal distortion was noted beginning at P17, while the peripheral retina demonstrated a trend of thinning from P12 to P30. We found that L-HIPR was associated with delayed and abnormal retinal vascular development with subsequent retinal inflammation, pericyte loss and preretinal fibrosis.

Conclusion
Our study presents a detailed analysis of the L-HIPR animal model demonstrating vitreoretinal pathologic changes, preretinal fibrosis and persistent hyaloidal vessels into adulthood. Based on our findings, we suggest that the persistence and peculiar stepwise migration of the hyaloidal vessels into the retina may provide a potential rescue mechanism for inner retinal development that deserves further study.
Associations between retail food environment and the nutritional quality of food purchases in French households: The Mont’Panier cross-sectional study
Authors: Recchia Daisy , Perignon Marlène , Rollet Pascaline , Vonthron Simon , Tharrey Marion , Darmon Nicole , Feuillet Thierry , Méjean Caroline ,   on behalf of the Surfood-Foodscapes working group
Published: April 2022

PurposeThe purpose of this study was to assess whether the retail food environment, measured by multiple indicators around the home and in activity space, was associated with the nutritional quality of food purchases.MethodsThis cross-sectional study included 462 households from a quota sampling survey conducted in the south of France (Montpellier Metropolitan Area). The revised Healthy Purchase Index was implemented in order to assess the nutritional quality of food purchases. Food environment indicators (presence, number, relative density and proximity of food outlets) were calculated around the home and in activity space using a geographical information system. Six different types of food outlets were studied: supermarkets, markets, greengrocers, bakeries, other specialized food stores (butcher’s, fishmonger’s and dairy stores) and small grocery stores. Associations between food environment and the nutritional quality of food purchases were assessed using multilevel models, and geographically weighted regressions to account for spatial non-stationarity. Models were adjusted for households’ socioeconomic and demographic characteristics.ResultsThe nutritional quality of food purchases was positively associated with the number of greengrocers around the home (1 vs. 0: β = 0.25, 95%CI = [0.01, 0.49]; >1 vs. 0: β = 0.25, 95%CI = [0.00, 0.50]), but negatively associated with the number of markets around the home (1 vs. 0: β = -0.20, 95%CI = [-0.40, 0.00]; >1 vs. 0: β = -0.37, 95%CI = [-0.69, -0.06]). These associations varied across space in the area studied. For lower income households, the number of greengrocers in activity space was positively associated with the nutritional quality of food purchases (1 vs. 0: β = 0.70, 95%CI = [0.12, 1.3]; >1 vs. 0: β = 0.67, 95%CI = [0.22, 1.1]).ConclusionsGreengrocers might be an effective type of food store for promoting healthier dietary behaviors. Further studies, particularly interventional studies, are needed to confirm these results in order to guide public health policies in actions designed to improve the food environment.
Biochemical diagnosis of Wilson’s disease: an update
Authors: Martínez-Morillo Eduardo , Bauça Josep Miquel 
Published: April 2022

Abstract
Wilson’s disease (WD) is an inherited disorder of copper metabolism caused by mutations in the ATP7B gene. This condition is characterized by the accumulation of copper in the liver and other organs and tissues causing hepatic and neuropsychiatric manifestations. This paper reviews the diagnostic performance and limitations of the biochemical tests commonly used to detect this underdiagnosed disease. It also provides some recommendations and suggests a set of standardized laboratory comments. At present, a rapid, simple, reliable biochemical test that confirms diagnosis of WD is not available. However, diagnosis can be established based on serum ceruloplasmin and urinary copper excretion. Total serum copper should be employed with caution, since it has a low negative predictive value. The use of estimated non-ceruloplasmin-bound copper is not recommended. Nevertheless, measured relative exchangeable copper has very high sensitivity and specificity and emerges as a potential gold standard for the biochemical diagnosis of WD. The development of novel assays for WD detection makes this disorder a potential candidate to be included in newborn screening programs.
Zum Verhältnis zwischen dem chinesischen Modalverb yīnggāi und dem Tempus sowie dem Aspekt
Authors: Zeng Zhen 
Published: April 2022

Abstract
This paper addresses the relation between the Chinese modal verb yīnggāi on one hand and tense and aspect on the other by providing illustrations of syntactial relations in Chinese sentences. It provides a detailed analysis of the different readings of yīnggāi in combination with various aspect particles and different time levels. It explains when the modal has a deontic, epistemic or ambiguous interpretation.
Contemporary Chinese art on WeChat and its utilisation to promote artists and their artworks
Authors: Zhang Cziráková Daniela 
Published: April 2022

Abstract
Social media are becoming increasingly popular in China. This trend is also observable in the art world. Besides specialised websites promoting art and artists, there are also many new ways of sharing information about artists, exhibitions, publications and the art world. Almost all Chinese artists use the WeChat application as a means of communication. There are also other social media outlets, such as Weibo and TikTok. This paper focuses on the use of WeChat by Chinese artists. Apart from its primary functionality, WeChat has become a vital medium for sharing information about recent exhibitions, articles or just to share artwork. WeChat allows artists to distribute and provide a space for new creative content. Artists and galleries can post photographs, links to articles, interviews and video promotions of exhibitions. The 798 Art Zone is an example of quite advanced promotion ranging from information about artists and exhibitions to even the sale of artworks. To illustrate the wide range of content on WeChat, this paper will look at visual artists such as Gu Wenda, Zhang Yu and the art critic Xia Kejun, and compare them with the public accounts of galleries and 798 Art zone (Beijing), the most famous art district in China.
Vṛddhekṣvākuvratam. The commentators’ interpretations of the passages describing the renunciation of kings in the Raghuvaṃśa
Authors: Dezső Csaba 
Published: April 2022

Abstract
At the end of their life, after transferring the kingdom to their grown-up sons, several kings of the Sūryavaṃśa chose to retire from the world and devoted their last years to asceticism and meditation. The verses of the Raghuvaṃśa describing these kings show considerable variation mainly along the lines of which āśrama (stage of life) the king enters at the end of his life. In many cases the commentators discuss these variants and argue pro and contra the availability of saṃnyāsa for people of royal status, thus participating in a larger mediaeval debate observable in texts on dharmaśāstra. This paper takes into consideration former studies on the subject by Tsuchida, Olivelle and Goodall, and most importantly discusses both published and unpublished commentaries on the Raghuvaṃśa, examining what authorities they quote to give support to their views on this dharmaśāstric issue, as well as the efforts they make to present the Raghuvaṃśa as being both internally consistent and in harmony with the teachings on dharma they consider valid.
The intra-individual variation of cardiac troponin I: the effects of sex, age, climatic season, and time between samples
Authors: Koerbin Gus , Potter Julia M. , Pinto do Nascimento Marcela , Cullen Louise , Scanlan Samuel L. , Woods Catherine , Hickman Peter E. 
Published: April 2022

Abstract

Objectives
Knowing the intra-individual variation (CVi), also termed within subject biological variation, of an analyte is essential to properly interpret apparent changes in concentration. While there have been many studies assessing the CVi of cardiac troponin (cTnI), they have been limited in looking at CVi in different settings, and there is no data available on whether CVi might change in different settings.

Methods
We used our large cTnI data bank to look at the CVi of cTnI in Emergency Department (ED) patients who had an acute myocardial infarction event excluded. We looked at the effects of gender, age, climatic season, and time between samples to assess whether CVi changed. To assess the effect of age, after exclusion, we collected two samples from each subject for each study which were used to calculate the CVi between those identified groups. There were 139 males and 98 females aged <65 years and 109 males and 98 females aged ≥65 years. For gender and season, there were 122 males and 94 females in the summer period and 126 males and 102 females in the winter period. To assess long term variation there were 195 males and 153 females who had further admissions after more than 12 months.

Results
For the four variables listed, there were no significant differences in within individual variation (CVi), but there was a significant difference in between individual variation (CVg) for men and women with regard to age. The Index of Individuality (II) was <0.20 for all conditions studied. We noted that >90% of subjects had an reference change value (RCV) <9 ng/L.

Conclusions
Because troponin concentration in patients without an identified cardiac condition change so little, delta changes are potentially of great value in assessing patients in the ED. Significant delta changes in troponin can occur without the 99th percentile being exceeded.
Modified optimal series cascade control for non-minimum phase system
Authors: Yadav Manish , Patel Hiren G. 
Published: April 2022

AbstractThis article contributes to handling the Non-Minimum Phase (NMP) system with time delay in the existence of uncertainty and disturbances. The series cascade control scheme is used to overcome such problems. The secondary loop architecture in a series cascade scheme is formulated in the Internal Model Control (IMC) framework. The tuning of fractional-filter via a delayed version of Bodes’ ideal transfer function approach of primary loop controller in a series cascade arrangement shows the novelty of this work. The primary loop controller is designed in the IMC framework after accountability of inverse response and dead-time compensator. Furthermore, Particle Swarm Optimization (PSO) is adapted to accomplish the optimal value of controller settings. The Riemann sheet principle is used to determine stability. The sensitivity investigation is performed to know the robustness of the offered controller. For the effectiveness of the suggested scheme, two case studies are revealed in this paper.
“Trust in These Words”: Vision, Voice, and Black Women’s Ways of Knowing
Authors: Wallace Belinda Deneen 
Published: April 2022

Abstract
Under the umbrella of what Brittney Cooper calls “progressive feminist visions,” this article reads Solange’s 2017 essay (“A Letter to My Teenage Self”) and her 2016 album (A Seat at the Table) as part of a Black feminist agenda to unfetter and embolden Black women to affirm their voices, visions, and knowledge(s). In doing this work, Solange makes visible how said voices, visions, and knowledge(s) are not only meaningful but also transformative. “Trust in these words” opens by establishing Solange’s work as progressive feminist visions. It then moves into an analysis of the role of vision and voice in crafting new ways of being and becoming as represented in the epistolary essay, “A Letter to My Teenage Self” and the album A Seat at the Table. The study closes by reflecting on how Solange’s work can be seen as Black feminist epistemologies that allow us to amplify Black women’s humanity.
Effect of heat-treatment processes and high temperature variation of acid-chloride media on the corrosion resistance of B265 (Ti–6Al–4V) titanium alloy in acid-chloride solution
Authors: Loto Roland Tolulope 
Published: April 2022

AbstractCorrosion resistance of untreated (B265TiN), quenched (B265TiQ), and annealed (B265TiA) B265 titanium alloy was studied in 2–10 M H2SO4+ 10% NaCl solution at 30, 70, and 95°C by potentiodynamic polarization, potentiostatic measurement, open circuit potential measurement, and optical microscopy. B265TiN was the most resistant of the alloys to general corrosion at 30°C with values between 0.094 and 3.782 mm/year. B265TiQ exhibited the highest corrosion rate values (0.210–23.399 mm/year). Its plots show significant increase in cathodic slope. At 70 and 95°C, B265TiN exhibited the lowest corrosion rate in 2 and 6 M H2SO4+ 10% NaCl solution, while B265TiQ exhibited the corresponding highest values. Optical characterization shows B265TiN, B265TiQ, and B265TiA alloys are highly resistant to corrosion in 2 M H2SO4+ 10% NaCl at 30°C compared to 10 M H2SO4+ 10% NaCl, where marginal deterioration occurred on B265TiN, significant degradation on B265TiQ, and localized degradation on B265TiA. The extent of degradation increased at 70 and 95°C. B265TiN exhibited the highest resistance to metastable pits formation compared to B265TiQ and B265TiA. Potentiostatic values at 70°C shows high temperature exposure of Ti alloy to accelerated corrosion. Metastable pit initiation values at 70°C are significantly greater. Open circuit potential measurement at 30°C shows B265TiN demonstrated least vulnerability to corrosion with stable oxide formation at 2 M and 6 M H2SO4+ 10% NaCl, and B265TiQ was least vulnerable at 10 M H2SO4+ 10% NaCl. At 70°C, B265TiQ was least vulnerable to corrosion with unstable oxide formation at 2 M and 6 M H2SO4+ 10% NaCl compared to B265TiN at 10 M H2SO4+ 10% NaCl.
Absolute convergence of the Fourier trigonometric series with gaps
Authors: Meskhia Rusudan 
Published: April 2022

Abstract
In the present paper, the sufficient conditions are obtained for the generalized β-absolute convergence

(

0
<
β
<
2

)

{(0<\beta<2)}

of the Fourier trigonometric series with gaps for some classes of functions. In [8], analogous problems were considered for Fourier trigonometric series and sufficient conditions were established in terms of the δ-variation of a function; also, it was proved that these conditions are unimprovable in a certain sense.
Our goal is to show that if a function f has a Fourier series with gaps, then the results obtained in [8] hold if the function f satisfies the derived conditions only on an arbitrarily small interval.
What is the action of a multiplicative centrally-extended derivation on a ring?
Authors: Tammam El-Sayiad M. S. , Ageeb Ahmed , Khaled A. M. 
Published: April 2022

Abstract
The purpose of this paper is to prove the following assertion.
Let S be a ring having a nontrivial idempotent element e which
satisfies some conditions. We prove that any multiplicative centrally-extended derivation of S
is a centrally-extended derivation.