bluuebunny/crossref_metadata_2025_split huggingface dataset:
The use of local raw materials and fermented feeds for the growth of Giant Prawns (Macrobrachium rosenbergii)
Authors: Mulyasari  , Setijaningsih L , Samsudin R , Taufik I , Kusmini I I 
Published: July 2022

Abstract
The purpose of this study was to evaluate the effect of local raw materials feed and fermented feed on the growth of giant prawns. This study was conducted using a completely randomized design (CRD) consisting of 3 treatments with 3 replications, each treatment was (A) commercial feed as a control; (B) feed based on local raw materials and (C) feed based on fermented local raw materials. The study was conducted at Sukawening Village, Dramaga District, Bogor Regency. The giant prawn that used has an initial weight of 0.31±0.005 g with stocking density of 50 fish/m2. The container used was a tarpaulin pond measuring 2x3x0.5m2. The study was conducted for 53 days. Observation variables include growth parameters, feed conversion, protein and fat retention, body amino acids and digestive tract enzyme activity. The results showed that feed based on local raw materials was able to produce relative growth (620±10.43%), specific growth rate (3.80±0.28%/day), protein retention (43.26%) and fat retention (36.1%) higher than other treatments and gave the lowest feed conversion value (1.07) compared to commercial feed and feed based on fermented local raw materials. These results indicate that feed based on local raw materials potential to be applied to giant prawn culture.
Characteristics of turtle laying habitat on vemara beach, Banyuwangi Regency, East Java
Authors: Isroni W , Maulida N , Irfan Muhajir M , Surya Lesmana P , Aji Ismail D 
Published: July 2022

Abstract
Turtles have a unique behavior, namely laying eggs on the same beach where they hatched. Turtles as protected animals continue to experience a decline in population caused by various factors including the degradation of nesting habitats. This study aims to determine the condition of the Cemara Beach habitat as a turtle nesting habitat. The research was conducted during September-November 2020 with a survey method by measuring the parameters of the Cemara Beach habitat which include sand temperature, air temperature, air humidity, beach width, beach slope, vegetation types and predators. The data collected was then analyzed descriptively qualitatively. The average air temperature is 28.07oC, the average sand temperature at a depth of 50 cm is 29.95oC, the average air humidity is 71.53%, the average beach width is 40 m and the average beach slope is 11.80 which shows the sloping beach category. The types of vegetation that grow along the coast are ketapang (Terminalia catappa), sea pine (Casuarina equisetifolia), horse tread (Ipomoea pes-caprae), cikumpai cikarao (Lepturus repens). As for the presence of predators identified during the observation, namely ghost crabs (Ocypode cursor).
The effect of salinity on the expression of heat shock protein 70 (hsp70) in the gills and kidneys of srikandi tilapia (Oreochromis niloticus)
Authors: Winarti M , Sulmartiwi L , Lutfiyah L 
Published: July 2022

Abstract
Heat Shock Protein 70 (HSP70) is the main stress protein synthesized by fish where there is a stressor, one of which is salinity. This study aims to determine the effect of salinity on the ekspression of HSP70 in the gills and kidneys of srikandi tilapia using the ELISA method. The parameter observed was the expression of HSP70 in the gills and kidneys of srikandi tilapia. These parameters were analyzed using one way factor ANOVA. The lower HSP70 expression was found in the P3 and the highest was found in P5 treatment. There was no difference in the gills and kidneys in the P1, P2, P3 treatments. However, the three treatments were significantly different freom the P4 and P5 treatment.
Analysis of organic waste loading (total suspended solid) from shrimp culture activity in mangara bombang coastal area – South Sulawesi Province
Authors: MHR Alauddin 
Published: July 2022

Abstract
The aims of this research is to analysis of organic waste (Total Suspended Solid) loading from shrimp culture activity are to Intensive, Semi Intensive, dan Traditional Plus. The methods employed in the research are; (i) field survei for shrimp culture technology and feed management applied in Mangara Bombang coastal area; (ii) Estimated Waste Organic Loading (Total Suspended Solid) based on Using Feed. Results of analysis to intensive shrimp culture (126 shrimp/m2), intensive shrimp culture (50 shrimp/m2), semi intensive shrimp culture (25 shrimp/m2), and traditional plus shrimp culture (8 shrimp/m2) show that generated for used feed are 10.058,25 kg/hectare, 2.789,54 kg/hectare, 1.681,4 kg/hectare, and 658,67 kg/hectare. While waste organic loading (Total Suspended Solid) obtained the generated are 9228.519 kg TSS/hectare, 2387.462 kg TSS/hectare, 1155.287 kg TSS/hectare, and 487.90 kg TSS/hectare.
Distribution of microalgae and omega-3 as food for Sardinella lemuru in Bali Strait
Authors: Suprakto B , Harijono T , Yudana I G P G R , Kiswanto A , Fauziah A 
Published: July 2022

Abstract
Sardinella lemuru is a small pelagic fish that contains high omega-3 which is distributed in the eastern Indian Ocean including the Bali Strait. Lemuru including high-fat with omega-3 content. This different fat content depends on the size of the fish, maturity, season, and type of food. The research objectives were to obtain abundance and diversity of microalgae composition as food and determine the level of omega-3 in the stomach of lemuru. The research was conducted at the 8 station with different waters characteristic. The data obtained were analized using t-test. The omega 3 content of lemuru in the second transitional season was 35.25% higher than the fish caught in the western season (21.4%). Lemuru feed on phytoplankton dominated by the Bacillariophyceae group with Chaetoceros gracilis in the second transitional season. These phytoplankton suspected important role as raw materials for forming omega 3 in the body of lemuru.
The abundance of alien fish species flowerhorn (Cichlasoma trimaculatum (GÜNTHER, 1867) in its fishing ground area at Lake Mahalona, South Sulawesi
Authors: Nasution S H , Muchlis A M , Cinnawara H T 
Published: July 2022

Abstract
Lake Mahalona is located in Towuti District, South Sulawesi Province. There are 29 species of fish found in Lake Mahalona and most of them are endemic fish. The presence of alien species deliberately or unexpected is one of threats causing the declining of the number of biodiversity in fresh waters. This study aims to determine the relative abundance of alien fish species Flowerhorn in its fishing ground area at Lake Mahalona. The research was conducted from January to June 2021 at six stations. Fish samples were obtained by using gill nets. The total Flowerhorn caught during the study were 15,677, the most caught in January was 3,346 and the least in June was 1,451. The Flowerhorn in January were the most number of fish at Kusapa (735), February and March at Muara (566 and 618), April at Tandumata (593), May and June at Badilla (434 and 375). The highest number and relative abundance of Flowerhorn fish were found at Kusapa in January (735 and 33.23%), while the lowest number and relative abundance of Flowerhorn fish were found at Lengkomali in June (129 and 4.62%). Flowerhorn as a alien fish species is distributed throughout the Lake Mahalona station. Flowerhorn fish occupy a lot of habitats with lots of aquatic plants, sandy and rocky substrates.
Evaluation of three species coral (Acropora branching) transplantation, case study; pantai tirtawangi Banyuwangi East Java
Authors: Anggara D P , Rahardja B S , Suciyono  
Published: July 2022

Abstract
The coral reef plays an important role in the marine ecosystem. These ecosystems are currently in decline due to a variety of factors such as irresponsible fishing, live coral capture for commercial purposes, seawater acidification, and others. Several attempts have been made to improve the situation, one of which was the transplantation method. Three Acropora species, Acropora Brueggemanni, Acropora Nobilis, and Acropora yongei, were planted at the best depth using the PVC rack method (5 meters) Tirtawangi Beach is located in Banyuwangi, East Java, Indonesia. We tracked the survival and growth of three transplanted corals on a monthly basis. A descriptive analysis was performed to determine the relationship between both and environmental parameters. The survival and growth rates of three Acropora corals transplanted at the optimal depth were discovered to be different for each species. Meanwhile, the three species' survival rates ranged from 96 to 100 percent, and their growth rates (mean SD) were 0.35 ± 0.11, 0.81 ±0.39, 0.47 ±0.15 cm.month-1, respectively. Furthermore, fragment adaptability to environmental changes is a major factor determining fragment growth, survival, and regeneration.
Distribution and Habitat Characteristics Of Endemic Fish Bilih (Mystacoleucus padangensis Blkr.) In Lake Singkarak, West Sumatra
Authors: Nasution Syahroma Husni , Suhaemi Syawal M. , Akhdiana Ira 
Published: July 2022

Abstract
Lake Singkarak is one of the volcanic lakes in Solok Regency, West Sumatra Province. Bilih (Mystacoleucus padangensis) is an endemic fish species and has important economic value in Lake Singkarak. Concerning will be occured degradation of the fish population in nature due to change of environment and intensive fishing. The aim of this research to obtain information on distribution and habitat characteristics of endemic fish Bilih in Lake Singkarak on May 2021. The fish captured with experimental gillnet mesh size 0.625; 0.75; and 1.0 inches at four stations. The number of Bilih fish caught during the study was 521 fish, most were found at Sumani station was 332 fish with a grain value of 45% sand, 37% silt, and 18% clay. The highest average length of male and female Bilih were 77.9 and 80.7 mm, and the highest average weight size of male and female fish was 41 and 46 mg, respectively, which was found at Sumani station. The highest GMS IV Bilih were found at Sumani station and at Paninggahan station dominated GMS III. The length and weight of male Bilih in GMS IV were the highest at 77.3 mm and 42 mg as well as female Bilih in GMS IV were at 80.6 mm and 46 mg at Sumani station. Bilih are distributed in all stations in the waters of Lake Singkarak. In general, the measurement results of water quality in Lake Singkarak during the study ranged from 27.87 – 28.91 °C for temperature, pH 8.59 – 9.24, Dissolved Oxygen ranged from 4.56 – 7.02 mg/L, Oxidation Reduction Potential ranged 30 – 155 mV, Conductivity ranges from 0.137 – 0.145 mS/Cm, Total Dissolved Solid ranges from 0.089 – 0.093 mg/L and the Sechi depth ranges from 2.27 – 3.78 meters.
Study on modification of nano silica by [3- (2-Aminoethyl) aminopropyl] trimethoxy silane (KH-792) to reinforce polymer film based on epoxy resin Epon 1001X75
Authors: Le Huy Cuong Huynh , Thi Thu Thao Nguyen 
Published: July 2022

Abstract
The surface of nano silica (NS) was modified by [3- (2-Aminoethyl) aminopropyl] trimethoxy silane (KH-792) to increase surface activity. Evaluate the efficiency of grafting [3- (2-Aminoethyl) aminopropyl] trimethoxy silane (KH-792) into NS was characterized by Fourier Transform Infrared Spectrometry (FTIR) and Thermo Gravimetric Analysis (TGA). NS and modified nano silica (m-NS) were dispersed into epoxy resin Epon 1001X75 by mechanical stirring and ultrasonic vibration. Epoxy resin Epon 1001X75 was cured with Epikure 3125 (Epi3125). The structural morphology and dynamic mechanical properties of materials were characterized by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Dynamic Mechanical Analysis (DMA). The results showed that NS was modified with the content of 20 wt% KH-792 for the highest denaturation efficiency. With adding the content of 1 wt% NS (NS-1), the mechanical properties of epoxy polymer film Epon 1001X75/Epi3125/NS-1 increased the impact strength from 20kG.cm to 52.5 kG.cm (increased by 162.5%). Adding the content of 2 wt% m-NS (m-NS-2) improved the impact strength of epoxy polymer film Epon 1001X75/Epi3125/m-NS-2 from 20 kG.cm to 55 kG.cm (increased by 175%). The glass transition temperature (Tg) of epoxy polymer composites Epon 1001X75/Epi3125, Epon 1001X75/Epi3125/NS-1, and Epon 1001X75/Epi3125/m-NS-2 gradually increased by 48 °C, 75 °C, and 79 °C. The obtained results showed that m-NS with KH-792 increased the mechanical properties of epoxy polymer film Epon 1001X75/Epi3125.
Characterization of low-temperature-grown ZnO nanoparticles: The effect of temperature on growth
Authors: Dejene F B 
Published: July 2022

Abstract
The growth temperature of semiconducting nanoparticles is well known to be an essential factor in determining their structure and quality of characteristics. To create high-quality ZnO nanostructures, it is critical to optimize the growth temperature. The sol-gel approach is used to render ZnO NPs at different growth temperatures in this report. The influence of growth temperature on the material properties of ZnO nanoparticles has been extensively investigated. The scanning electron microscopy (SEM) images show needle-like structures when synthesized at low temperatures (35 and 45 °C) that transformed into spherical particles as the temperature of the growing medium increased to 75 °C. X-ray diffraction (XRD) analysis displays an increase in crystallinity with annealing temperatures. The average crystallite sizes of ZnO nanoparticles rose with increasing growth temperature and varied from 28 to 34 nm. The XRD peaks positions shift slightly towards lower 2θ angles with the growth temperatures brought about by a change in lattice parameters. The Fourier transform infrared spectroscopy (FTIR) study revealed the presence of a Z–O bond at around 680 cm−1. The intensity of the defect level emission (DLE) band decreased, as the growth temperature exceeded 35 °C. The photoluminescence (PL) study further demonstrated that the prepared nanoparticles had a strong emission peak at 546 nm. PL measurements confirm that with increasing growth temperature, the excitonic peak intensity increases, indicating that the quality of ZnO nanoparticles improves. The estimated bandgap changed from 3.31 to 3.24 eV with the growth temperature.
Metal accumulation in muscle and oxidative stress response in the liver of juvenile Oreochromis niloticus from contaminated sediment under a simulation of increasing temperature
Authors: Ihunwo O C , Ibezim-Ezeani M U 
Published: July 2022

Abstract
In the present study, a mesocosm experiment with contaminated sediment and clean groundwater using juvenile Oreochromis niloticus was set up to assess the effect of increasing temperature on bioaccumulation of metal in fish muscles (cadmium, chromium, nickel, and lead) and enzymatic activities in fish liver [reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione transferase (GST), and glutathione peroxidase (GSH Px)]. The trend of temperature variation was Control 1 (0 Watts) < 60 Watts < 100 Watts < Control 2 (200 Watts). After 72 h, there was no observed mortality in Controls 1 and 2; however, the % Survival in 0 Watts reduced to 90.0 ± 4.0%. Furthermore, 60, 100 and 200 Watts had lower rates of survival at 46.0 ± 6.9, 36.0 ± 13.1, and 24.0 ± 11.1% respectively. The calculated bioaccumulation factors (BAFs) for metals was in the trend: Pb > Cd > Cr > Ni. Mean metal pollution index (MPI) in fish muscles was lowest at 0 watts (24 h − 7.86 ± 1.72, 72 h − 25.77 ± 4.56) and highest at 100 watts (24 h − 39.45 ± 1.91, 72 h − 55.82 ± 1.05). Controls 1 and 2 showed no significant difference in the concentration of GSH and GR while showing a significant difference in GST and GSH Px concentrations after 24 and 72 h. Pearson’s correlation showed that GSH was inhibited by water temperature and, Cd, Cr, and Pb concentrations in surface water and fish. Therefore, an increase in ambient temperature in an already contaminated environment will increase the bioavailability of metal contaminants leading to an increase in bioaccumulation and exacerbation of oxidative stress in juvenile tilapia.
Adenovirus-vectored SARS-CoV-2 vaccine expressing S1-N fusion protein
Authors: Khan Muhammad S , Kim Eun , McPherson Alex , Weisel Florian J , Huang Shaohua , Kenniston Thomas W , Percivalle Elena , Cassaniti Irene , Baldanti Fausto , Meisel Marlies , Gambotto Andrea 
Published: July 2022

Abstract
Additional COVID-19 vaccines that are safe and immunogenic are needed for global vaccine equity. Here, we developed a recombinant type 5 adenovirus vector encoding for the SARS-CoV-2 S1 subunit antigen and nucleocapsid as a fusion protein (Ad5.SARS-CoV-2-S1N). A single subcutaneous immunization with Ad5.SARS-CoV-2-S1N induced a similar humoral response, along with a significantly higher S1-specific cellular response, as a recombinant type 5 adenovirus vector encoding for S1 alone (Ad5.SARS-CoV-2-S1). Immunogenicity was improved by homologous prime-boost vaccination, and further improved through intramuscular heterologous prime-boost vaccination using subunit recombinant S1 protein. Priming with low dose (1 × 1010 v.p.) of Ad5.SARS-CoV-2-S1N and boosting with either wild-type recombinant rS1 or B.1.351 recombinant rS1 induced a robust neutralizing response, which was sustained against Beta and Gamma SARS-CoV-2 variants. This novel Ad5-vectored SARS-CoV-2 vaccine candidate showed promising immunogenicity in mice and supports the further development of COVID-19-based vaccines incorporating the nucleoprotein as a target antigen.
Mesh-fixation technique for inguinal hernia repair: umbrella review
Authors: Techapongsatorn Suphakarn , Tansawet Amarit , Pattanaprateep Oraluck , Attia John , Mckay Gareth J , Thakkinstian Ammarin 
Published: July 2022

Abstract

Background
Mesh-based repair is the standard of surgical care for symptomatic inguinal hernias. Many systematic reviews and meta-analyses (SRMAs) addressed various aspects of these procedures. This umbrella review aimed to report the evidence from all previous SRMAs for open and laparoscopic inguinal hernia repair.

Methods
SRMAs were identified from MEDLINE, Scopus, Cochrane, Embase, DARE, PROSPERO, CINAHL, JBISRIS, EPPI-Centre, Wiley Online Library and ScienceDirect database according to PRISMA guidelines. Data including mesh-fixation techniques and surgical approach were extracted from selected SRMAs. The corrected covered area was calculated to address study overlap across reviews, and an excess significance test was used to assess potential bias. The outcomes of interest were hernia recurrence, chronic groin pain, operating time, postoperative pain, duration of hospital stay, return to daily life activities, and postoperative complication.

Results
Thirty SRMAs were included between 2010 and 2019: 16 focused on open repair, and 14 focused on laparoscopic repair, with a high degree of overlap (open repairs, 41 per cent; laparoscopic repairs, 30–57 per cent). Sufficient evidence was available on hernia recurrence, chronic groin pain, and operative time. Effects of glue on hernia recurrence were inconclusive in open and laparoscopy approaches, P = 0.816 and 0.946 respectively. Glue was significantly associated with lower persistent groin pain, in open repair (versus suture) and in laparoscopic repair (versus tack). SRMAs suggested that self-gripping mesh was associated with shorter operating time in open surgery, although with only a few minutes of improvement (0.36–7.85 min, P &lt; 0.001).

Conclusion
In this umbrella review, chronic groin pain and operating time were the only outcomes for which there was sufficient evidence supporting the effectiveness respectively of glue and self-gripping mesh.
Application of Supervised and Unsupervised Learning Approaches for Mapping Storage Conditions of Biopharmaceutical Product—A Case Study of Human Serum Albumin
Authors: Lalchandani Dimple S , Paritala Sreeteja , Gupta Pawan Kumar , Porwal Pawan Kumar 
Published: July 2022

AbstractThe stability of biopharmaceutical therapeutics over the storage period/shelf life has been a challenging concern for manufacturers. A noble strategy for mapping best and suitable storage conditions for recombinant human serum albumin (rHSA) in laboratory mixture was optimized using chromatographic data as per principal component analysis (PCA), and similarity was defined using hierarchical cluster analysis. In contrast, separability was defined using linear discriminant analysis (LDA) models. The quantitation was performed for rHSA peak (analyte of interest) and its degraded products, i.e., dimer, trimer, agglomerates and other degradation products. The chromatographic variables were calculated using validated stability-indicating assay method. The chromatographic data mapping was done for the above-mentioned peaks over three months at different temperatures, i.e., 20°C, 5–8°C and at room temperature (25°C). The PCA had figured out the ungrouped variable, whereas supervised mapping was done using LDA. As an outcome result of LDA, about 60% of data were correctly classified with the highest sensitivity for 25°C (Aq), 25°C and 5–8°C (Aq with 5% glucose as a stabilizer), whereas the highest specificity was observed for samples stored at 5–8°C (Aq with 5% glucose as a stabilizer).
A case report of giant cell myocarditis after a syncope-related motor vehicle accident: an atypical presentation for a life-threatening condition
Authors: Pham Lily A , Chung Joseph , Gavaghan Callan 
Published: July 2022

Abstract

Background
Giant cell myocarditis (GCM) is a rare and rapidly progressive disease associated with significant morbidity and mortality. Whilst patients more frequently present with acute heart failure, diagnosis is difficult due to heterogeneity in clinical presentations.

Case summary
This case report presents a previously healthy 59-year-old Vietnamese woman who initially presented with syncope and a motor vehicle accident who developed rapid decline in left ventricular function. Her initial echocardiogram was suggestive of an infiltrative cardiomyopathy. GCM was confirmed on biopsy, and she received combined immunosuppression. Twenty-seven days following her initial presentation to hospital, she was unable to recover from severe multi-organ dysfunction, and the patient was palliated and passed away.

Discussion
This case highlights the varied manner in which GCM may present. Even in the absence of cardiogenic shock at presentation, giant cell myocarditis should be considered in the evaluation of new cardiomyopathy of uncertainty aetiology. Diagnosis of this condition has distinct clinical implications on management and prognosis.
Is local and organic produce less satiating? Some evidence from a field experiment
Authors: Franceschinis Cristiano , Scarpa Riccardo , Rossetto Luca , Thiene Mara 
Published: July 2022

Abstract
We investigate consumers’ preferences towards local and organic food via a framed field experiment involving revealed multiple discrete–continuous choices. Participants were endowed with a cash amount as a budget to purchase any desired quantity of different products. We modelled choices via the multiple discrete–continuous nested extreme value model. Central to our investigation is the test of the hypothesis of the constant effect of attitudes across consumption doses, which is normally an assumption invoked a priori and without testing in discrete choice analyses. Our results support the hypothesis and reveal a strong preference towards organic and local products, associated with both the highest baseline utility and the lowest satiation effect.