bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrooxidation and subcritical water oxidation hybrid process for pistachio wastewater treatment
Authors: Isik Zelal , Jabbiyev Zhalaladdin , Bouchareb Raouf , Ozay Yasin , Yabalak Erdal , Dizge Nadir 
Published: May 2022

Abstract
In this study, a hybrid system was used for pistachio wastewater treatment based on the electrooxidation treatment process and subcritical water oxidation. The effects of current density (50–150 A/m 2 ), experiments time (0–180 min), and wastewater initial pH (4-8) were optimized for maximum total phenols and chemical oxygen demand (COD) removal. The experimental study proved that the best conditions of current density and solution pH for COD and total phenol removal efficiencies were 150 A/m 2 and original pH of 5 giving removal efficiencies of 50% and 19.02%, respectively. The electrochemically pretreated wastewater was subjected to subcritical water oxidation (SWO). The effects of temperature (376–410 K), treatment time (26.4-93.65 min), and concentration of H 2 O 2 (0.08-0.92 M) were examined for COD and total phenol removal. The experimental and predicted COD and total phenol removal of the central composite design (CCD) models demonstrated that temperature, treatment time and concentration of H 2 O 2 have highly affected each of COD and total phenol removals. The highest removal efficiencies were as much as 54.3% and 76.87% for COD and total phenol at the following optimum conditions: temperature of 403 K, 40 min of treatment time and 0.75 M of H 2 O 2 concentration. The SWO results showed that, although treatment time affects the removal yields, it is not as effective as hydrogen peroxide concentration. In addition, it was found that COD and total phenol removal yields at the lowest values of all variables remained at 18.36% and 44.58%, respectively. Besides, they increased to 42.17% and 53.17%, respectively, by increasing only treatment time to its highest level. However, further increasing the treatment time after a specific level, may not increase the removal yields. Nevertheless, higher removal rates in both COD and total phenol were achieved even in the lowest level of the treatment time, but the highest level of the other two variables.
Capturing SARS-CoV-2 from patient samples with low viral abundance: a comparative analysis
Authors: Fonseca Juliana Pipoli da , Kornobis Etienne , Turc Elodie , Enouf Vincent , Lemée Laure , Cokelaer Thomas , Monot Marc 
Published: May 2022

Abstract
Since the beginning of the SARS-CoV-2 coronavirus pandemic, genome sequencing is essential to monitor viral mutations over time and by territory. This need for complete genetic information is further reinforced by the rapid spread of variants of concern. In this paper, we assess the ability of the hybridization technique, Capture-Seq, to detect the SARS-CoV-2 genome, either partially or in its integrity on patients samples. We studied 20 patient nasal swab samples broken down into five series of four samples of equivalent viral load from CT25 to CT36+. For this, we tested 3 multi-virus panel as well as 2 SARS-CoV-2 only panels. The panels were chosen based on their specificity, global or specific, as well as their technological difference in the composition of the probes: ssRNA, ssDNA and dsDNA. The multi-virus panels are able to capture high-abundance targets but fail to capture the lowest-abundance targets, with a high percentage of off-target reads corresponding to the abundance of the host sequences. Both SARS-CoV-2-only panels were very effective, with high percentage of reads corresponding to the target. Overall, capture followed by sequencing is very effective for the study of SARS-CoV-2 in low-abundance patient samples and is suitable for samples with CT values up to 35.
Comparison of four commercially available thyroid peroxidase autoantibody and two thyroglobulin autoantibody assays
Authors: Wolffenbuttel Bruce H.R. , Wouters Hanneke J.C.M , Kobold Anneke C. Muller , Roozendaal Caroline , Klauw Melanie M. 
Published: May 2022

Abstract
Background:
Measurement of thyroid peroxidase autoantibodies (TPOAb) is used to confirm a diagnosis of Hashimoto’s hypothyroidism. Presence of TPOAb may predict future thyroid dysfunction, and is also associated with adverse pregnancy outcomes and worse quality of life.
Methods:
We evaluated the performance of four TPOAb and two thyroglobulin antibodies (TgAb) assays in 71 patients with a presumed or established thyroid disorder with a large variety of TPOAb concentrations (range < 33-3204 IU/ml, originally measured with Thermo Fisher Phadia Cap assay). In addition, we reviewed the reference values of all assays. These samples were re-measured with the Abbott ARCHITECT, Roche Cobas and Phadia EliA assays, and also evaluated for TgAb with BRAHMS TgAb KRYPTOR and Phadia TgAb EliA. Passing-Bablok regression analysis plots were created to assess the differences between the assays.
Results:
Of 18 individuals negative for TPOAb with the Phadia Cap, three were positive with Abbott Architect, and four with both the Roche Cobas and Phadia EliA. All individuals with detectable TPOAb > 33 IU/ml in the Phadia Cap also had detectable levels with the three other assays. In general, these other assays reported 1.2-1.8x higher TPOAb concentrations than the Phadia Cap. Five of the 18 individuals with negative TPOAb showed positive TgAb with the Brahms, and 5 with the Phadia EliA assay. Of the 53 individuals with detectable TPOAb with Phadia Cap, 11 did not show TgAb with the Brahms assay, and 13 with the Phadia EliA. Overall, the correlation between both TgAb assays was poor.
Conclusions:
Different TPO and Tg auto-antibody assays quantitively and qualitatively yield different results. These differences may have consequences for daily clinical practice.
Explore the attitudes of children and adolescent parents towards the vaccination of COVID-19 in China
Authors: Wang Lin , Wen Wen , Chen Chen , Tang Jiake , Wang Chunyi , Zhou Mengyun , Cheng Yongran , Zhang Xingwei , Wang Mingwei , Feng Zhanhui , Wang Weiqian 
Published: May 2022

Abstract
Background
With the increasing incidence of asymptomatic carriers or milder symptoms, children and adolescents are likely to become a silent source of infection. In view of the efficacy and safety of vaccines in the treatment of novel Coronavirus pneumonia, population-wide vaccination will be an inevitable trend to control the spread of COVID-19. However, there is no survey on the attitudes of Chinese parents of children and adolescents towards their children's COVID-19 vaccination.
Methods
We used online questionnaires to find out the attitudes of Chinese parents toward their children's immunization against COVID-19. Logistic regression was used to explore the influencing factors.
Results
A total of 2019 parents participated in the survey. Overall, 74.38% parents said they would actively get vaccinated, 8.90% refused to get vaccinated, 4.60% said they would delay vaccination and 12.12% were still undecided.
Conclusions
In general, Chinese parents have a high desire to be vaccinated against COVID-19, and most parents have a positive attitude towards their children's vaccination. However, many people still hesitate or even refuse to be vaccinated. Education background, attitudes towards children's vaccination, children's age, recent illness and other factors have a certain impact on Chinese parents of children and adolescents towards their children's COVID-19 vaccination.
The effect of Thiazolidinediones on the vascular endothelium and plaque in diabetic patients with coronary atherosclerosis：a meta-analysis
Authors: Xue Cheng-Yuan , Zhou Meng-Qi , Zheng Qi-Yan , Cheng Wei-Ting , Bai Xue-Hui , Zhang Jin-Hui , Zhou Fen , Wu Ai-Ming , Nie Bo , Liu Wei-Jing , Lou Li-Xia 
Published: May 2022

Abstract
Rosiglitazone (Avandia) and Pioglitazone (Actos) belong to the class of thiazolidinedione drugs that act by increasing insulin sensitivity and are widely used for treating diabetic patients with insulin resistance. Thiazolidinediones (TZDs) exhibit anti-inflammatory and antioxidant properties and inhibit endothelial cell inflammation and dysfunction, and then play a role in inhibiting plaque formation and coronary atherosclerosis. But the results of evidence-based medicine suggest that thiazolinedione may increase the risk of myocardial infarction. To explore the dispute in depth, the meta-analysis aimed to evaluate the changes in vascular endothelial and plaque-related indicators following treatment with thiazolidinediones on diabetic patients with coronary atherosclerosis.According to our meta-analysis, Thiazolinediones showed a protective effect on the vascular endothelium and an inhibiting effect on plaque progression in patients with diabetes and coronary atherosclerosis and these effects may not depend on related targets or pathways such as inflammation and lipid regulation. Due to the poor quality of the evidence, more and higher-quality studies are needed to further improve the above conclusions.
Variables associated with progression of moderate-to-severe Crohn’s disease
Authors: Sacramento Carolina da Silva Beda , Motta Marina Pamponet , Alves Candida de Oliveira , Mota Jaciane Araujo , de Codes Lina Maria Goes , Liberato Renata Nobrega Cordeiro , Ferreira Reginaldo Freitas , Silva Pedro de Almeida , Palmiro Larissa do Prado , Barbosa Rafael Miranda , Andrade Mariana Nery , Andrade Vitor Damasceno , Vasconcelos Vitor Brandão , Thiara Bernardo Wasconcellos , Netto Eduardo Martins , Santana Genoile Oliveira 
Published: May 2022

Abstract

Objective: Currently, variables associated with Crohn’s disease progression remain insufficiently investigated in developing countries. Thus, this study aimed to determine the variables associated with hospitalizations in patients with Crohn’s disease and those associated with surgery, intestinal resection, hospital readmission, surgical recurrence, and immunobiological agent use.Results: A retrospective cross-sectional study was conducted from 2019 to 2021, using two centers for inflammatory bowel diseases in the Brazilian Public Health System. This study included 220 patients. Perianal disease was associated with hospitalization. Stricturing or penetrating behavior and perianal disease were associated with surgery. Ileal or ileocolonic location and stricturing or penetrating behavior were associated with intestinal resection. Furthermore, steroid use at first Crohn’s disease occurrence and postoperative complications were associated with hospital readmission and surgical recurrence, respectively. Age below 40 years at diagnosis, upper gastrointestinal tract involvement, and perianal disease were associated with immunobiological agent use. These findings are similar to those in the countries with a high prevalence of Crohn’s disease, and could be applicable in other developing countries. Knowing these variables is useful in identifying the severity profile of Crohn’s disease and selecting the most appropriate management and treatment.
Peppermint (Mentha piperita) extract modulates the genotoxic effects of Cypermethrin and Malathion insecticides on Allium cepa root cells
Authors: Amin Osman Ali Hag El , Nosiar Hala Ragab , Ibrahim Rashid Ismael Hag 
Published: May 2022

Abstract
The potential protective effect of peppermint (Mentha piperita; Labiatae) water extract was investigated on onion (Allium cepa) root tips that were exposed to cytotoxic and genotoxic insecticides; cypermethrin or malathion. Cypermethrin was tested at 0.019, 0.038 and 0.075mg/ml and malathion at 0.235, 0.469 and 0.938mg/ml, based on concentrations used in the field. Parallel doses of 4µg/ml or 8µg/ml peppermint extract were applied for each insecticide treatment, while distilled water was used as a control. Exposure time was 8 hours for all treatments. Mitotic index (MI), cell proliferation kinetics (CPK), percentage of mitotic aberrations (MAs) and nuclear aberrations (NAs) were evaluated. Both insecticides exerted a significant decline in MI (p < 0.001) and mitotic kinetics aberrations, which indicated potent cytotoxicity of both insecticides even at lower doses, where significant reduction of MI (more than 50% below the control) because of prophase imbalance and metaphase arrest. Both insecticides induced a concentration dependent MAs ranging between 19.0 and 29.6%. MAs and TAs of cypermethrin were significant at the high dose, while those caused by malathion were significant at both medium and high doses. Aberrations observed included bridges, vagrants, laggards, stickiness, multipolar, binucleated cells, micro-, lobed, blebbed and vacuolated nuclei. Application of peppermint water extract to both insecticides reduced the cytotoxic and genotoxic capacity of these insecticides.
Clinical Outcomes of a Medically-Tailored Meal and Medical Nutrition Therapy Pilot for Food-Insecure Patients with Congestive Heart Failure
Authors: Belak Lauren , Owens Caroline , Smith Margaret , Calloway Eric , Samnadda Laura , Egwuogu Heartley , Schmidt Stacie 
Published: May 2022

Abstract
Background: To evaluate the impact of home-delivered, medically tailored meals and medical nutrition therapy among food-insecure patients following hospitalization for congestive heart failure by comparing clinical outcomes to a retrospectively matched cohort. Methods: Patients at high risk for readmission and food insecurity received up to three months of medically tailored meals and medical nutrition therapy after discharge. Pre-intervention and post-intervention weight, body mass index, blood pressure, and dietary intake were assessed. A combination of difference-in-difference and logistic regression models were used to compare changes between cohorts and evaluate impact attributable to the program.Results: Thirty-nine program participants were compared to a matched cohort of 117 unexposed patients. Participants experienced a marginal reduction in body mass index and an increase in systolic and diastolic blood pressure; however, these results were not statistically significant. To determine relevance to clinical cut-offs, logistic regressions were used, demonstrating that exposure to the intervention resulted in higher odds of a categorical reduction in blood pressure (OR: 1.85), though this did not reach statistical significance (95% CI: 0.67-5.32). Pre vs. post trends indicated that more-healthful foods and drinks increased numerically or remained similar to baseline, while less-healthful foods decreased numerically or remained similar to baseline.Conclusions and Implications: These findings highlight the need for more longitudinal research on medically tailored meals and medical nutrition therapy interventions using clinical outcomes while setting realistic suggestions for program implementation. This study additionally illustrates the promise of integrating electronic medical record data and matched cohorts into medical nutrition program evaluation within the health sector.
The amount of circulating DNA and the presence of TP53 mutations in plasma predict clinical outcome of renal cancer patients treated with immunotherapy and VEGFR-TKIs
Authors: Re Marzia Del , Crucitta Stefania , Paolieri Federico , Cucchiara Federico , Verzoni Elena , Bloise Francesco , Ciampi Raffaele , Mercinelli Chiara , Capuano Annalisa , Sportiello Liberata , Procopio Giuseppe , Galli Luca , Porta Camillo , Bracarda Sergio , Danesi Romano 
Published: May 2022

Abstract
Background Despite the increasing treatments options, reliable biomarkers of response are still missing for patients affected by metastatic renal cell carcinoma (mRCC). Methods Patients affected by mRCC undergoing standard first line treatment were enrolled. Twelve ml of blood were drawn at treatment baseline and circulating free DNA (cfDNA) was extracted from plasma. Next-generation sequencing (NGS) analyses were performed on cfDNA using the Pan-Cancer Cell-Free Assay and clinical outcomes were correlated with liquid biopsy. Results A total of 48 patients were enrolled, 12 received immunotherapy and 36 received a vascular endothelial growth factor receptor (VEGFR) tyrosine kinase inhibitor (TKI). A cfDNA cut-off of 0.883 ng/µl stratified patients based on progression-free survival (PFS) and overall survival (OS) (p=0.001 and p=0.008, respectively). cfDNA amount was also correlated with best response (p=0.006). A further cfDNA cut-point divided patients into short, intermediate and long responders, with a PFS of 4.87 vs 9.13 vs 23.1 months, respectively (p<0.001). PFS resulted to be shorter in carriers of mutant TP53 compared to not carriers with a p=0.04. Patients with high cfDNA levels and TP53 mutant have the worst PFS, and patients with low cfDNA and no mutations in TP53 have the longer PFS (p=0.004). Conclusions The present study demonstrates that cfDNA and TP53 may be used as predictive biomarkers of response in mRCC.
Challenges, strategies, and opportunities for wind farm incorporated power systems: a review with bibliographic coupling analysis
Authors: Evangeline Sundarapandi Edward Ida , Rathika Ponpandi 
Published: May 2022

Abstract
The development of wind power is progressing at a rapid pace. On a global scale, it is one of the most quickly expanding sources of power. Many countries rely on wind energy for a considerable portion of their energy needs. Its technology is constantly evolving. The security of supply and sustainability goals are achieved by increasing the installation of wind power capacity and utilization of wind resources. Because of this, the incorporation of wind power into the electrical grid is becoming a topic of research. Four significant topics within the theme of wind farm incorporated power systems are assessed. Their technical challenges and solutions are outlined in this paper. The topics include the effect of the energy storage system, optimization of wind farm layout, wind power forecasting, and impact on optimal power flow. Furthermore, a bibliographic coupling analysis is presented. It shows the interactions between journals and the natural impact of the journals. It also indicates the productivity of scientific journals, citations, dissemination, and diversification of influence.
Epstein-Barr virus-associated acute pancreatitis: a clinical report and review of literature
Authors: Accomando Salvatore , Restivo Giulia Angela , Scalzo Simona , Guardino Melania , Corsello Giovanni , Giuffrè Mario 
Published: May 2022

Abstract
Background: Acute pancreatitis is a disorder of reversible inflammation of the pancreas. Only few cases are related to infections and the most common pathogens are the viruses responsible for mumps, parotitis, and influenza. Epstein-Barr virus (EBV)-associated acute pancreatitis is a rare condition, both in children and adults.Case presentation: A 3-year-old female was admitted to our hospital for vomiting and abdominal pain. Laboratory investigations revealed elevated amylase and lipase, with normal liver function tests. Abdomen ultrasound demonstrated an enlarged pancreas, with hypoechogenic areas; no biliary lithiasis was observed. Infectious disease serology was positive for the presence of EBV VCA IgM and IgG. A diagnosis of EBV-associated acute pancreatitis was made. The patient was treated conservatively and she recovered. Conclusions: Acute pancreatitis is rarely associated with EBV infection; a review of the English literature revealed only 10 pediatric and 6 adult cases. Patients with pancreatitis should always be evaluated for EBV serology, even in the absence of the typical clinical and hematological features of infectious mononucleosis. For these patients, good prognosis is generally expected.
Prevalence of and factors associated with unmanageable pain levels in people with knee or hip osteoarthritis: a cross-sectional population-based study
Authors: Costa Daniela , Cruz Eduardo B , Lopes David G , Silva Catarina Nunes da , Henriques Ana Rita , Luis Diogo , Branco Jaime , Canhão Helena , Rodrigues Ana M 
Published: May 2022

Abstract
Background
Pain due to knee or hip osteoarthritis (HKOA) is the most common symptom for seeking health care given its interference on daily activities, social and occupational participation. The goal of this study is to estimate the prevalence of unmanageable pain levels (UPL) among people with hip and/or knee osteoarthritis (HKOA), characterize this population and identify factors associated with UPL, and compare therapeutic strategies used by people with UPL versus manageable pain levels (MPL).
Methods
We analyzed a representative sample of 1081 participants with a validated diagnosis of HKOA, from the population-based study EpiReumaPt. Sociodemographic, lifestyle and health-related data were collected in a structured interview. Pain intensity (NPRS) data were collected in a medical appointment. UPL was defined as a mean pain intensity in the previous week of ≥ 5 points on 11-point numeric pain rating scale. Intake of regular medication for pain relief (last month), physiotherapy and surgery were considered as therapies for pain management. The factors associated with UPL were analyzed with logistic regression (p < 0.05, 95%CI). To assess the effects of unmanageable pain levels in the HOOS/KOOS activities of daily living and quality of life subscales and in the presence of anxiety and depression symptoms, linear and logistic regression were used. All analysis were weighted.
Results
The estimated prevalence of UPL among people with HKOA was 68.8%. UPL was associated with being female (odds ratio (OR) = 2.36, p < 0.01), being overweight (OR = 1.84, p = 0.035) or obese (OR = 2.26, p = 0.006), and having multimorbidity (OR = 2.08, p = 0.002). People with UPL reported worse performance in activities of daily living and lower quality of life (β=-21.28, p < 0.001 and β=–21.19, p < 0.001, respectively) than people with MPL. People with UPL consumed more NSAIDs (22.0%, p = 0.003), opioids (4.8%, p = 0.008), paracetamol (2.7%, p = 0.033), and overall analgesics (7.3%, p = 0.013) than people with MPL. A higher proportion of people with UPL underwent physiotherapy (17.5%, p = 0.002) than people with MPL.
Conclusion
These results indicate that most people with HKOA have poor control of pain, highlighting the need for further research and implementation of effective interventions.
Long noncoding RNA LINC01132 enhances immunosuppression and therapy resistance via NRF1/DPP4 axis in hepatocellular carcinoma
Authors: Zhang Jiwei , Pan Tao , Zhou Weiwei , Zhang Ya , Xu Gang , Xu Qi , Li Si , Gao Yueying , Wang Zhengtao , Xu Juan , Li Yongsheng 
Published: May 2022

Abstract
Background
Long noncoding RNAs (lncRNAs) are emerging as critical regulators of gene expression and play fundamental roles in various types of cancer. Current developments in transcriptome analyses unveiled the existence of lncRNAs; however, their functional characterization remains a challenge.
Methods
A bioinformatics screen was performed by integration of multiple omics data in hepatocellular carcinoma (HCC) prioritizing a novel oncogenic lncRNA, LINC01132. Expression of LINC01132 in HCC and control tissues was validated by qRT-PCR. Cell viability and migration activity was examined by MTT and transwell assays. Finally, our results were confirmed in vivo mouse model and ex vivo patient derived tumor xenograft experiments to determine the mechanism of action and explore LINC01132-targeted immunotherapy.
Results
Systematic investigation of lncRNAs genome-wide expression patterns revealed LINC01132 as an oncogene in HCC. LINC01132 is significantly overexpressed in tumor and associated with poor overall survival of HCC patients, which is mainly driven by copy number amplification. Functionally, LINC01132 overexpression promoted cell growth, proliferation, invasion and metastasis in vitro and in vivo. Mechanistically, LINC01132 acts as an oncogenic driver by physically interacting with NRF and enhancing the expression of DPP4. Notably, LINC01132 silencing triggers CD8 + T cells infiltration, and LINC01132 knockdown combined with anti-PDL1 treatment improves antitumor immunity, which may prove a new combination therapy in HCC.
Conclusions
LINC01132 functions as an oncogenic driver that induces HCC development via the NRF1/DPP4 axis. Silencing LINC01132 may enhance the efficacy of anti-PDL1 immunotherapy in HCC patients.