bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nucleolar targeting in an early-branching eukaryote suggests a general mechanism for ribosome protein sorting
Authors: Jeilani Milad , Billington Karen , Sunter Jack Daniel , Dean Samuel , Wheeler Richard John 
Published: September 2022

ABSTRACT
The compartmentalised eukaryotic cell demands accurate targeting of proteins to the organelles in which they function, whether membrane-bound (like the nucleus) or non-membrane-bound (like the nucleolus). Nucleolar targeting relies on positively charged localisation signals and has received rejuvenated interest since the widespread recognition of liquid–liquid phase separation (LLPS) as a mechanism contributing to nucleolus formation. Here, we exploit a new genome-wide analysis of protein localisation in the early-branching eukaryote Trypanosoma brucei to analyse general nucleolar protein properties. T. brucei nucleolar proteins have similar properties to those in common model eukaryotes, specifically basic amino acids. Using protein truncations and addition of candidate targeting sequences to proteins, we show both homopolymer runs and distributed basic amino acids give nucleolar partition, further aided by a nuclear localisation signal (NLS). These findings are consistent with phase separation models of nucleolar formation and physical protein properties being a major contributing mechanism for eukaryotic nucleolar targeting, conserved from the last eukaryotic common ancestor. Importantly, cytoplasmic ribosome proteins, unlike mitochondrial ribosome proteins, have more basic residues – pointing to adaptation of physicochemical properties to assist segregation.
The histone methyltransferase SETD2 negatively regulates cell size
Authors: Molenaar Thom M. , Malik Muddassir , Silva Joana , Liu Ning Qing , Haarhuis Judith H. I. , Ambrosi Christina , Kwesi-Maliepaard Eliza Mari , van Welsem Tibor , Baubec Tuncay , Faller William J. , van Leeuwen Fred 
Published: September 2022

ABSTRACT
Cell size varies between cell types but is tightly regulated by cell intrinsic and extrinsic mechanisms. Cell size control is important for cell function, and changes in cell size are frequently observed in cancer. Here, we uncover a role for SETD2 in regulating cell size. SETD2 is a lysine methyltransferase and a tumor suppressor protein involved in transcription, RNA processing and DNA repair. At the molecular level, SETD2 is best known for associating with RNA polymerase II through its Set2-Rbp1 interacting (SRI) domain and methylating histone H3 on lysine 36 (H3K36) during transcription. Using multiple independent perturbation strategies, we identify SETD2 as a negative regulator of global protein synthesis rates and cell size. We provide evidence that overexpression of the H3K36 demethylase KDM4A or the oncohistone H3.3K36M also increase cell size. In addition, ectopic overexpression of a decoy SRI domain increased cell size, suggesting that the relevant substrate is engaged by SETD2 via its SRI domain. These data add a central role of SETD2 in regulating cellular physiology and warrant further studies on separating the different functions of SETD2 in cancer development.
Actin turnover protects the cytokinetic contractile ring from structural instability
Authors: McDargh Zachary , Zhu Tianyi , Zhu Hongkang , O'Shaughnessy Ben 
Published: September 2022

ABSTRACT
In common with other actomyosin contractile cellular machineries, actin turnover is required for normal function of the cytokinetic contractile ring. Cofilin is an actin-binding protein contributing to turnover by severing actin filaments, required for cytokinesis by many organisms. In fission yeast cofilin mutants, contractile rings suffer bridging instabilities in which segments of the ring peel away from the plasma membrane, forming straight bridges whose ends remain attached to the membrane. The origin of bridging instability is unclear. Here, we used molecularly explicit simulations of contractile rings to examine the role of cofilin. Simulations reproduced the experimentally observed cycles of bridging and reassembly during constriction, and the occurrence of bridging in ring segments with low density of the myosin II protein Myo2. The lack of cofilin severing produced ∼2-fold longer filaments and, consequently, ∼2-fold higher ring tensions. Simulations identified bridging as originating in the boosted ring tension, which increased centripetal forces that detached actin from Myo2, which was anchoring actin to the membrane. Thus, cofilin serves a critical role in cytokinesis by providing protection from bridging, the principal structural threat to contractile rings.
Selective Addition of Aniline to a Cage-opened Diketo Anhydride Derivative of C60
Authors: Hashikawa Yoshifumi , Sadai Shumpei , Li Jiayue , Okamoto Shu , Murata Yasujiro 
Published: September 2022

Abstract
The nucleophilic addition of aniline was examined using a diketo cage-opened C60 derivative possessing an acid anhydride moiety. While the imination occurred at the 1,4-dicarbonyl moiety, the aminalization dominantly proceeded on the anhydride moiety in the presence of zinc(II). The thus formed aminal was thermally converted into acid anhydride while it could be transformed into corresponding isoimide by the use of acid catalyst. The introduced aniline moiety significantly perturbed the orifice size so that the insertion/escape dynamics of a water molecule were substantially restricted.
Use of organic fertilizers in solar photo-Fenton process as potential technology to remove pineapple processing wastewater in Costa Rica
Authors: Bolaños Picado Dayatri Vanessa , Masis Mora Mario , Duran Herrera Esteban , Pérez Mercado Luís Fernando , López Vinent Núria , Cruz Alcalde Alberto , Alvarez Caero María Mercedes , Rodríguez Rodríguez Carlos Esteban , Sans Mazón Carmen 
Published: September 2022

Background: This work studied the use of the organic fertilizers DTPA-Fe and EDDS-Fe as iron chelates for solar driven photo-Fenton process at natural pH. This process was proposed to investigate its performance on removing a mixture of agrochemicals (propiconazole, imidacloprid and diuron) from pineapple processing wastewater to obtain a suitable effluent to be reused in the agricultural sector. Methods: Experiments were carried out in a solar simulator with a stirred cylindric photoreactor, with a volume of 150 mL and controlled temperature (20°C). The first set of experiments was carried out with ultrapure water to determine optimal iron and H2O2 concentrations. The second was performed with simulated wastewater of pineapple processing. Results: The optimized operational conditions for both iron complexes were 10 mg L-1 of Fe (III) and 25 mg L-1 of H2O2, since more than 80% of micropollutants (MP) (at an initial concentration of 1 mg L-1 of each compound) were removed in only 20 min with both DTPA-Fe and EDDS-Fe. The effect of organic matter and inorganic salts on radicals scavenging and chelates stability was also investigated in the experiments performed with synthetic pineapple processing wastewater. The results disclosed differences depending on the iron complex. Nitrites were the principal component influencing the tests carried out with EDDS-Fe. While carbonates at low concentration only significantly affected the experiments performed with DTPA-Fe, they were the major influence on the MPs removal efficiency decrease. In contrast, the presence of Ca2+ and Mg2+ only influence on this last one. Finally, the results of phytotoxicity disclosed the suitability of treated effluent to be reused in the agricultural sector. Conclusions: This work demonstrated that solar powered photo-Fenton catalysed by iron fertilizer EDDS is a suitable technology for depolluting water streams coming from pineapple processing plants at circumneutral pH, and its subsequent reuse for crop irrigation.
Designing and Assessing a Multidisciplinary Service-Learning Course in Supply Chain Management
Authors: Natarajarathinam Malini , Qiu Shaoping , Lu Wei 
Published: September 2022

In this paper, we describe an innovative design for a multidisciplinary service-learning course on supply chain management in the industrial distribution program housed in the College of Engineering at a large research university in Texas. The purpose of this course was to increase students’ technical expertise and build a mindset and skills needed to tackle current societal issues, raising their social responsibility and enhancing civic-mindedness. The course combined curricular learning with community service, applying the content of academic disciplines to practice. It brought students from industrial distribution and sociology together to help food pantries to improve their operational efficiencies so that the food pantries can serve more people and work toward their goal. In this project, student groups identified the specific needs of community food pantries. They recommended solutions to improve operational efficiency so that they could use their technical skills to address the challenges and make an impact on the communities. In addition, student groups interacted with partner agencies via technologies that aided distance communication, which facilitated partnerships in remote locations. We also evaluated this project. The results reveal that this project had an impact on students’ personal and professional development as well as the community. Funding: We acknowledge the tier-one priority grant funding from the provost office, dean of faculty, and department of student activities at Texas A&M University.
Asthma and self-management: Improving support for teenagers
Authors: Duncan Debbie 
Published: September 2022

The prevalence and severity of allergic diseases is still rising in those aged 11–25 years ( Venkataraman et al, 2018 ; Mallol et al, 2019 ). An estimated 800 000 teenagers in the UK have asthma. This figure could be higher as teenagers can have undiagnosed asthma ( Siersted et al, 1998 ; BTS/SIGN, 2019).A person-centred approach to all aspects of care is central to supporting adolescents to self-manage their asthma and therefore minimise concerns around morbidity and mortality. School nurses have a key role in communicating strategies that support self-management, and the use of tools can be helpful.
Water Productivity of Irrigated Tomatoes in Eastern Canada Based on AquaCrop Simulations
Authors: Thangaraju Arumugagounder Naresh Kumar , Ihuoma Samuel , Madramootoo Chandra A. 
Published: September 2022

HighlightsMeasured field harvest index improved the performance of AquaCrop simulations.Optimal irrigated tomato yield can be achieved by maintaining available soil water depletion below 25%.Water productivity for tomatoes in humid regions can be suitably simulated using AquaCrop.Irrespective of the soil type, the water productivity was highest for fully irrigated fields compared to water limiting irrigated fields.Abstract. Methodologies to predict crop water requirements in arid and semi-arid regions are well known. Humid regions such as eastern Canada pose a challenge because irrigation is normally only required for short periods during the growing season to supplement rainfall. This study assessed the capability of the AquaCrop model to simulate the effects of different irrigation regimes on field-grown tomatoes (Solanum lycopersicum L.) in the humid region of eastern Canada. The experimental study was conducted at the Horticultural Research station of McGill University, Quebec, Canada. There were three irrigation treatments in 2017 and in 2019, that were based on the % depletion of available water content (AWC). The AquaCrop model was calibrated and verified with the 2017 and 2019 field data, respectively. The verified model was used to predict irrigation water requirements and fruit yield for the driest year (1993) and the average rainfall year (2001) of a 35-year historic weather dataset from 1986 to 2000, for three different soil types (silty clay, sandy loam, and heavy clay) under four irrigation regimes. Model performance was greatly improved when the seasonal harvest index (HI) measured from experimental data was used instead of the default model HI values. AquaCrop was suitable for estimating dry yield and total biomass with RMSE = 0.57 ton ha-1 and 0.89 ton ha-1, respectively, in the calibration phase, and RMSE = 0.28 ton ha-1 and 0.01 ton ha-1 for dry yield and total biomass, respectively, in the verification phase. These results indicate a very high accuracy of AquaCrop to estimate total above ground biomass and fruit yield in humid regions with seasonally adjusted HI values. The predictions showed that maintaining AWC depletion below 25% resulted in no significant decrease in crop yield and biomass, making it an optimum water management guideline for irrigated tomato production in Quebec. The findings of this study are useful to crop growers and water resource managers in eastern Canada, who seek better irrigation strategies to optimize productivity. Keywords: AquaCrop, Crop Modeling, Humid climate, Irrigation water requirement, Tomatoes, Yield.
An ecosystem approach to manage pelagic thresher shark (Alopias pelagicus) based in Kutaraja Ocean Fishing Port, Aceh
Authors: Mardhatillah Inda , Taurusman Am Azbas , Sondita Muhammad Fedi Alfiadi , Fajri Ilham , Aris Muhammad 
Published: September 2022

Sharks is the dominant bycatch of purse seine and bottom longline vessels that landed in Kutaraja ocean fishing port, particularly pelagic thresher shark species (Alopias pelagicus). The management of shark fisheries in Aceh water is not well implemented. According to the IUCN red list, the pelagic thresher shark has been classified as an endangered species. The capture and trade of pelagic thresher sharks are regulated by CITES (Appendix II). So that Indonesia has to comply with the procedures of CITES due to it has been adopted through the Decree of Minister of Marine Affairs and Fisheries of the Republic of Indonesia Number 61/PERMEN-KP/2018. However, the exploitation and trading of the A. pelagicus continues and is not properly managed. It will threaten the extinction of the pelagic thresher shark population and in terms of a negative image of fisheries management in Indonesia, in particular. Thus, integrated management such as the ecosystem approach to fisheries management (EAFM) is needed to solve this problem. This study aims to evaluate the fisheries management status of the pelagic thresher shark based on EAFM indicator of fish resources domain, focusing in the Kutaraja ocean fishing port. This research was conducted by field measurement and interviews with key stakeholders. Data were analyzed using a multi-criteria analysis (MCA) approach through development of a composite index. The results showed that the trend of CPUE tends to fluctuate in the last 5 years. The total length of the sharks was relatively constant. The proportion of juvenile pelagic thresher sharks was 16% male, and 28% (female), and the shark was bycatch (1%) of the purse seine fishery, the fishing ground was getting further away and seemed to go close to the shark habitat. Based on this assessment, one could conclude that shark's fishery resources were in 'good' category, however, some indicators need to improve through better fisheries management actions. Keywords: EAFM; Alopias pelagicus; pelagic thresher shark; Kutaraja ocean fishing port
Application of pumpkin flour to the diet of Barramundi Lates calcarifer fingerling
Authors: Yanti Riska Z. , Muchlisin Zainal A. , Muhammadar Abdullah A. , Fadli Nur 
Published: September 2022

Barramundi Lates calcarifer is a high economic value fish in Indonesia and Southeast Asia countries. However, the aquaculture system still experiences problems, including the high cost of production, specifically from feed, which is due to its relatively slow growth and low digestibility. Therefore, this study aims to determine alternative ingredients that can be used in the fish diet to improve feed utilization and growth performance using pumpkin flour Cucurbita moschata. A completely randomized design (CRD) with 6 treatments and 3 replications was adopted in this study. The tested diet was (A) diet without pumpkin flour (control), (B) with 5% (C) 10% (D) 15% (E) 20%, and (F) 25%. The size of the Barramundi samples was 3-4 cm on average and the body weight was 1-3 g at the stocking density of 15 fish/container. The fish fed as much as 10% body weight daily, which is divided into 2 times, namely 08.00 AM and 4.00 PM for 60 days. The results showed that the addition of pumpkin flour had a significant effect on weight gain, daily and specific growth rate, survival, feed efficiency, conversion ratio, and carcass protein (P0.05). The diet with 5% pumpkin flour gave the best results over other treatments, with an average weight gain of 0.761 g, feed conversion of 1.578, feed efficiency of 63.455%, and a daily and specific growth rate of 0.012 g and 0.738%, respectively, The highest carcass protein content was found in the control treatment with a value of 18.210%, which was not significantly different from the 5% pumpkin flour treatment. Therefore, it is concluded that pumpkin flour can be added to the Barramundi diet as much as 5%. Keywords: Barramundi, Snapper, pumpkin, feed utilization
Multi-Dimensional Hadamard-Transform Fluorescence Imaging
Authors: Katz Oren G. , Loock Hans-Peter 
Published: September 2022

Using a multiplexing technique, we have built an instrument that captures excitation emission matrices (EEM) for every pixel in an image, gaining information over four dimensions (x,y,λex,λem) and reduces acquisition time 500-fold from conventional designs.
Comments and questions should be directed to the Optica Conference Papers staff (tel: +1 202.416.6191, e-mail: cstech@optica.org).
High-sensitivity and wide-bandwidth fiber-coupled diamond magnetometer with surface coating
Authors: Zhang Shao-Chun , Lin Hao-Bin , Dong Yang , Du Bo , Gao Xue-Dong , Yu Cui , Feng Zhi-Hong , Chen Xiang-Dong , Guo Guang-Can , Sun Fang-Wen 
Published: September 2022

Mapping magnetic fields from different materials and structures can provide a powerful means for broad applications of activity probe and feature analysis. Here, we present a high-sensitivity and wide-bandwidth fiber-based quantum magnetometer at the scale of a few hundred micrometers. We propose a fiber-coupled diamond magnetometer. Tracking a pulsed optically detected magnetic resonance spectrum allows a magnetic field sensitivity of

103

pT
/

Hz

and a bandwidth of 2.6 kHz. Additionally, with an approach of coating the diamond surface with silver reflective film, both the fluorescence collection and excitation efficiency are significantly enhanced, and the sensitivity and bandwidth are expected to be further improved to

50

pT
/

Hz

and 4.1 kHz, respectively. Finally, this fiber-based quantum magnetometer is applied as a probe to successfully map the magnetic field induced by the current-carrying copper-wire mesh. Such a stable and compact magnetometer can provide a powerful tool in many areas of physical, chemical, and biological researches.
Long term follow up of direct oral anticoagulants and warfarin therapy on stroke, with all-cause mortality as a competing risk, in people with atrial fibrillation: Sentinel network database study
Authors: de Lusignan Simon , Hobbs F. D. Richard , Liyanage Harshana , Sherlock Julian , Ferreira Filipa , Tripathy Manasa , Heiss Christian , Feher Michael , Joy Mark P. 
Published: September 2022

Background
We investigated differences in risk of stroke, with all-cause mortality as a competing risk, in people newly diagnosed with atrial fibrillation (AF) who were commenced on either direct oral anticoagulants (DOACs) or warfarin treatment.

Methods and results
We conducted a retrospective cohort study of the Oxford Royal College of General Practitioners (RCGP) Research and Surveillance Centre (RSC) database (a network of 500 English general practices). We compared long term exposure to DOAC (n = 5,168) and warfarin (n = 7,451) in new cases of AF not previously treated with oral anticoagulants. Analyses included: survival analysis, estimating cause specific hazard ratios (CSHR), Fine-Gray analysis for factors affecting cumulative incidence of events occurring over time and a cumulative risk regression with time varying effects.We found no difference in CSHR between stroke 1.08 (0.72–1.63, p = 0.69) and all-cause mortality 0.93 (0.81–1.08, p = 0.37), or between the anticoagulant groups. Fine-Gray analysis produced similar results 1.07 (0.71–1.6 p = 0.75) for stroke and 0.93 (0.8–1.07, p = 0.3) mortality. The cumulative risk of mortality with DOAC was significantly elevated in early follow-up (67 days), with cumulative risk decreasing until 1,537 days and all-cause mortality risk significantly decreased coefficient estimate:: -0.23 (-0.38–0.01, p = 0.001); which persisted over seven years of follow-up.

Conclusions
In this large, contemporary, real world primary care study with longer follow-up, we found no overall difference in the hazard of stroke between warfarin and DOAC treatment for AF. However, there was a significant time-varying effect between anti-coagulant regimen on all-cause mortality, with DOACs showing better survival. This is a key methodological observation for future follow-up studies, and reassuring for patients and health care professionals for longer duration of therapy
The impact of an integrated, interprofessional knowledge translation intervention on access to inpatient rehabilitation for persons with cognitive impairment
Authors: Linkewich Elizabeth , Rios Jorge , Allen Kay-Ann , Avery Lisa , Dawson Deirdre R. , Donald Michelle , Egan Mary , Hunt Anne , Jutzi Katelyn , McEwen Sara 
Published: September 2022

Introduction
Stroke rehabilitation teams’ skills and knowledge in treating persons with cognitive impairment (CI) contribute to their reduced access to inpatient rehabilitation. This study examined stroke inpatient rehabilitation referral acceptance rates for persons with CI before and after the implementation of a multi-faceted integrated knowledge translation (KT) intervention aimed at improving clinicians’ skills in a cognitive-strategy based approach, Cognitive Orientation to daily Occupational Performance (CO-OP), CO-OP KT.

Methods
CO-OP KT was implemented at five inpatient rehabilitation centres, using an interrupted time series design and data from an electronic referral and database system called E-Stroke. CO-OP KT included a 2-day workshop, 4 months of implementation support, health system support, and a sustainability plan. A mixed effects model was used to model monthly acceptance rates for 12 months prior to the intervention and 6 months post.

Results
The dataset was comprised of 2604 pre-intervention referrals and 1354 post. In the mixed effects model, those with CI had a lower pre-intervention acceptance rate than those without. Post-intervention the model showed the acceptance rate for those with CI increased by 8.6% (p = 0.02), whereas those with no CI showed a non-significant increase of less than 1%.

Conclusions
Proportionally more persons with CI gained access to inpatient stroke rehabilitation following an integrated KT intervention.