bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of using platelet‐rich fibrin to increase keratinized tissue around the tooth in the modified apical reposition flap method: A split‐mouth, randomized controlled trial
Authors: Torkzaban Parviz , Rabienejad Nazli , Cheraghi Zahra , Lahoorpoor Kamran 
Published: November 2022

AbstractObjectivesOne of the simplest methods to increase keratinized gingiva is the modified apically repositioned flap (MARF) technique. In this method, the periosteum remains exposed, which may lead to postoperative pain and discomfort. In the presence of bone dehiscence, bone resorption and gingival recession may occur. Hence, this study aims to use platelet‐rich fibrin (PRF) to promote wound healing in the MARF technique and overcome its disadvantages.Material and MethodsIn this randomized controlled trial study, 10 patients (six males and four females with a mean age of 33.9 ± 11.13) with less than 2 mm of attached gingiva bilaterally were treated by the MARF + PRF membrane (test group), on the one hand, whereas, on the other hand, it was treated only by MARF (control group). Clinical parameters of keratinized gingiva include thickness, width, and vestibule depth before and 8 weeks after the intervention were measured.ResultsThe attached gingival width increased significantly in both groups (1.7 mm in the MARF and 2.3 mm in the PRF) and this was greater in the PRF group (p < .05). Gingival thickness in the PRF method was significantly higher than in the control group (p < .05). Probe depth, vestibular depth, and postoperative pain were not different in both groups. Wound shrinkage in the MARF group (51%) was significantly higher than in the PRF group (30%) (p < .05).ConclusionUsing PRF with the MARF method significantly increased the width and thickness of the gingiva and reduced shrinkage compared to MARF only. Postoperative pain and vestibular depth changes were similar in both groups.
Recent advances on the structure identification of components in food
Authors: Zhao Min , Tian Jin‐Long 
Published: November 2022

AbstractWith the rapid development of food industry, people pay more and more attention to the health benefits of food‐active molecules. The active function of food molecules depends on the chemical structure. So how to determine the chemical composition of food becomes very important. Based on this, this paper mainly expounds the structural analysis methods and specific processes of food chemical components, as well as the difficulties encountered in the determination of structure, and summarizes the latest research trends of food structural analysis. The purpose is to let more scholars understand the structure analysis process of the chemical substance basis of food, and provide a reference for the healthy development of food.
Dynamic aspects of ventricular interaction during exercise in HFpEF and in pre‐capillary pulmonary hypertension
Authors: Claeys Mathias , Petit Thibault , Bogaert Jan , La Gerche Andre , Los Jan , Delcroix Marion , Willems Rik , Claessen Guido , Claus Piet 
Published: November 2022

AbstractAimsThe contribution of adverse ventricular interdependence remains undervalued in heart failure or pulmonary vascular disease, and not much is known about its dynamic nature during exercise and respiration. In this study, we evaluated ventricular interaction during exercise in patients with heart failure with preserved ejection fraction (HFpEF) and patients with chronic thromboembolic pulmonary hypertension (CTEPH) as compared with healthy controls.Methods and resultsForty‐six subjects (10 controls, 19 CTEPH patients, and 17 HFpEF patients) underwent cardiac magnetic resonance imaging during exercise. Ventricular interaction was determined through analysis of the septal curvature (SC) of a mid‐ventricular short‐axis slice at end‐diastole, end‐systole, and early‐diastole, both in expiration and inspiration. Exercise amplified ventricular interaction in CTEPH patients and to a lesser extent in HFpEF patients (P < 0.05 for decrease in SC with exercise). Adverse interaction was most profound in early‐diastole and most pronounced in CTEPH patients (P < 0.05 interaction group * exercise) because of a disproportionate increase RV afterload (P < 0.05 to both controls and HFpEF) and diastolic pericardial restraint (P < 0.001 for interaction group * exercise) during exercise. Inspiration enhanced diastolic interdependence in CTEPH and HFpEF patients (P < 0.05 vs. expiration). Both at rest and during exercise, SC strongly correlated with RV volumes and pulmonary artery pressures (all P < 0.05).ConclusionsExercise amplifies adverse right–left ventricular interactions in CTEPH, while a more moderate effect is observed in isolated post‐capillary HFpEF. Given the strong link with RV function and pulmonary hemodynamic, assessing ventricular interaction with exCMR might be valuable from a diagnostic or therapeutic perspective.
Experimental testing of aluminum‐core buckling restrained knee braced Truss moment frames for earthquake resistance
Authors: Chhabra Jaskanwal P. S. , Rai Durgesh C. 
Published: November 2022

AbstractApplication of Buckling Restrained Braces (BRBs) with Truss Moment Frames (TMFs) results in a robust seismic force‐resisting system, herein referred to as Buckling Restrained Knee Braced Truss Moment Frame (BRKB‐TMF), which utilizes the advantages of both BRBs and TMFs. Although steel is commonly used to make the core of BRBs, previous studies have demonstrated the efficacy of annealed aluminum as a preferred core material because of its high ductility and high over‐strength. However, the deformation compatibility of the truss members with the aluminum‐core BRB in BRKB‐TMFs at high drifts is not well studied in the literature. Hence, an experimental study is performed on a scaled sub‐assemblage of a BRKB‐TMF frame designed using the Performance‐Based Plastic Design method. Cyclic tests are performed on 1:3 scale models of aluminum‐core BRB and BRKB‐TMF to evaluate their performance at drifts corresponding to Maximum Considered Earthquake intensity. Also, to further compare the performance of steel and aluminum as suitable BRB core materials, cyclic tests are performed on BRBs with steel and aluminum cores designed using a consistent criterion. The experiments demonstrated the superior performance of aluminum core BRB over steel core BRB. The experimental studies on the BRKB‐TMF concluded that per the design intent, the BRB acted like a fuse for the frame by restricting all the inelastic deformations to itself and the study frame could successfully meet the target drift criteria.
A novel advanced test system for polymer electrolyte membrane water electrolysis based on hydraulic cell compression
Authors: Rost Ulrich , Wirkert Florian Josef , Roth Jeffrey , Brodmann Michael , Stiber Svenja , Gago Aldo Saul , Friedrich Kaspar Andreas 
Published: November 2022

AbstractIn this work, a novel polymer electrolyte membrane water electrolyzer (PEMWE) test cell based on hydraulic single‐cell compression is described. In this test cell, the current density distribution is almost homogeneous over the active cell area due to hydraulic cell clamping. As the hydraulic medium entirely surrounds the active cell components, it is also used to control cell temperature resulting in even temperature distribution. The PEMWE single‐cell test system based on hydraulic compression offers a 25 cm2 active surface area (5.0 × 5.0 cm) and can be operated up to 80°C and 6.0 A/cm2. Construction details and material selection for the designed test cell are given in this document. Furthermore, findings related to pressure distribution analyzed by utilizing a pressure‐sensitive foil, the cell performance indicated by polarization curves, and the reproducibility of results are described. Experimental data indicate the applicability of the presented testing device for relevant PEMWE component testing and material analysis.
Partially different? The importance of general equilibrium in health economic evaluations: An application to nocturia
Authors: Hafner Marco , Yerushalmi Erez , Andersson Fredrik L. , Burtea Teodor 
Published: November 2022

AbstractBoth the human capital approach and the friction cost approach are frequently used to quantify the productivity costs associated with illness, disability or death in health economic evaluations. In this paper we argue that these approaches have one major, but common shortcoming: they only capture partial equilibrium (PE) effects and therefore underestimate the true potential productivity costs associated with health conditions. They neglect the sizable, indirect, ripple effects in the economy captured by general equilibrium (GE) models. To demonstrate our point, we compare a traditional PE with a GE approach for the application to nocturia, a condition characterized by the need to frequently wake up at night to urinate. Nocturia is associated with substantial impairment of daytime functioning and work productivity. We employ large‐scale United Kingdom (UK) employer‐employee survey data to estimate the prevalence and productivity loss. These estimates are then used as shared inputs to drive both approaches. We find that the traditional PE approach underestimates the annual productivity cost of clinically relevant nocturia by around 16%. We propose a generalized GE/PE multiplier to approximate the GE effect for other health conditions. Our findings stress the importance of accounting for sizable GE effects when conducting health economic evaluations.
The clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: A basic descriptive study
Authors: Robinson Shayene , Ngwenya Sharol , Molaudzi Mulalo , Molepo Julitha , Adeola Henry , Magangane Pumza 
Published: November 2022

AbstractBackground and AimsLymphovascular invasion (LVI) is an indicator of lymph node metastasis and poor prognosis in various cancers including squamous cell carcinoma (SCC). Despite being easily resectable and having little potential for LVI; SCC displays aggressive behavior and often results in the death of the patient. With this in mind, it may be useful to investigate the clinical, pathological, and microRNA expression profile associated with LVI in SCC.MethodsWe evaluated the histological hallmarks associated with LVI from 16 formalin fixed paraffin embedded (FFPE) tissue samples (10 LVI−, 6 LVI+). We also quantified the expression of 10 microRNAs (hsa‐miR‐21‐5p, hsa‐miR‐21‐3p, hsa‐miR‐155‐5p, hsa‐miR‐196a‐5p, hsa‐miR‐375, hsa‐let‐7d‐5p, hsa‐miR‐146b‐3p, hsa‐miR‐221‐5p, hsa‐miR‐205‐5p, hsa‐miR‐491‐5p), which have been previously identified to play a role in SCC development, using real time‐PCR with the Qiagen miRCURY LNA SYBR Green PCR Kit.ResultsWe observed a significant upregulation of microRNA‐155, microRNA‐196a, microRNA‐375, and microRNA‐221 in cases with lymphovascular invasion. Morphologically, we identified poor differentiation, dysplasia, loss of membrane polarity, high nuclear to cytoplasmic ratio, and the presence of squamous nests as defining features of LVI. Additionally, we found a gender bias and observed a tendency toward lymphatic invasion in lesions presenting around the perineal and abdominal regions.ConclusionWe speculate that this profile may have prognostic significance and could guide the clinician in their treatment protocols for patients matching our genetic, demographic, and morphologic profile.
TMEM173 rs7447927 genetic polymorphism and susceptibility to severe enterovirus 71 infection in Chinese children
Authors: Song Jie , Liu Yedan , Guo Ya , Qu Zhenghai , Liu Peipei , Li Fei , Yang Chengqing , Fan Fan , Chen Zongbo 
Published: November 2022

AbstractIntroductionThis study was designed to explore the association between the TMEM173 polymorphism (rs7447927) and the severity of enterovirus 71 (EV71) infection among Chinese children.MethodsThe TMEM173 polymorphism was identified in EV71‐infected patients (n = 497) and healthy controls (n = 535) using the improved multiplex ligation detection reaction (iMLDR). The interferon‐α (IFN‐α) serum levels were detected using enzyme linked immunosorbent assay (ELISA).ResultsThe frequencies of the GG genotype and G allele of TMEM173 rs7447927 in the mild EV71 infection and severe EV71 infection groups were markedly higher than those in the control group. The GG genotype and G allele frequencies in severely infected EV71 patients were significantly higher than those in mildly infected EV71 patients. Severely infected EV71 patients with the GG genotype had higher white blood cell counts (WBC), and C‐reactive proteins (CRP), and blood glucose (BG) levels, longer fever duration, higher vomiting frequency, spirit changes, and electroencephalography (EEG) abnormalities. IFN‐α serum concentration in severely infected patients was significantly higher than in the mildly infected group. The IFN‐α concentration in the GG genotype was significantly higher compared with those in the GC and CC genotypes in severe cases.ConclusionsThe TMEM173 rs7447927 polymorphism was associated with EV71 infection susceptibility and severity. The G allele and GG genotype are susceptibility factors in the development of severe EV71 infection in Chinese children.
Two formulations of coronavirus disease‐19 recombinant subunit vaccine candidate made up of S1 fragment protein P1, S2 fragment protein P2, and nucleocapsid protein elicit strong immunogenicity in mice
Authors: Özcengiz Erkan , Keser Duygu , Özcengiz Gülay , Çelik Gözde , Özkul Aykut , İnçeh Fatma Nur 
Published: November 2022

AbstractIntroductionCoronavirus disease (COVID‐19) is ongoing as a global epidemic and there is still a need to develop much safer and more effective new vaccines that can also be easily adapted to important variants of the pathogen. In the present study in this direction, we developed a new COVID‐19 vaccine, composed of two critical antigenic fragments of the S1 and S2 region of severe acute respiratory syndrome coronavirus 2 as well as the whole nucleocapsid protein (N), which was formulated with either alum or alum plus monophosphoryl lipid A (MPLA) adjuvant combinations.MethodsFrom within the spike protein S1 region, a fragmented protein P1 (MW:33 kDa) which includes the receptor‐binding domain (RBD), another fragment protein P2 (MW:17.6) which contains important antigenic epitopes within the spike protein S2 region, and N protein (MW:46 kDa) were obtained after recombinant expression of the corresponding gene regions in Escherichia coli BL21. For use in immunization studies, three proteins were adsorbed with aluminum hydroxide gel and with the combination of aluminum hydroxide gel plus MPLA.ResultsEach of the three protein antigens produced strong reactions in enzyme‐linked immunosorbent assays and Western blot analysis studies performed with convalescent COVID‐19 patient sera. In mice, these combined protein vaccine candidates elicited high titer anti‐P1, anti‐P2, and anti‐N IgG and IgG2a responses. These also induced highly neutralizing antibodies and elicited significant cell‐mediated immunity as demonstrated by enhanced antigen‐specific levels of interferon‐γ (INF‐γ) in the splenocytes of immunized mice.ConclusionThe results of this study showed that formulations of the three proteins with Alum or Alum + MPLA are effective in terms of humoral and cellular responses. However, since the Alum + MPLA formulation appears to be superior in Th1 response, this vaccine candidate may be recommended mainly for the elderly and immunocompromised individuals. We also believe that the alum‐only formulation will provide great benefits for adults, young adolescents, and children.
Telemedicine for home care of fetal growth restriction with mobile cardiotocography: A case series
Authors: Suemitsu Tokumasa , Kadooka Mizuho , Mitani Takahiro , Matsui Hiroki , Suzuki Makoto 
Published: November 2022

AbstractObjectiveModern technological advancements have made it possible to perform cardiotocography at home. Home‐based management of high‐risk pregnancies using a mobile cardiotocography system has been reported; however, its effectiveness in monitoring cases of fetal growth restriction (FGR) remains unclear. Therefore, the authors aimed to investigate the clinical usefulness of home‐based telemedicine for FGR management using the mobile cardiotocography (iCTG).MethodsThe authors conducted a single‐center, retrospective case series of patients with FGR. Seventeen women diagnosed with FGR were enrolled. Patients performed iCTG for 1 hour twice daily to examine their fetuses; data were uploaded and saved on the cloud.ResultsThe median and minimum compliance rates were 93.33 (interquartile range [IQR], 70.00–100.00) and 40.7, respectively. The median and minimum validity rates were 100.00 (IQR, 90.48–100.00) and 36.4, respectively. In this study, many of the patients were managed at home and underwent delivery as planned. However, three patients required emergency visits; one had a nonreassuring fetal status and underwent an emergency cesarean section.ConclusionEven when the fetal prognosis is good, careful pre‐evaluation is required before initiating home care management. The current study shows that the economic burden of hospitalization for patients can be reduced by using iCTG.
Hsa_circ_0000129 drives tumor growth via sequestering miR‐485‐3p and upregulating SPIN1 in breast cancer
Authors: Zhou Yuxin , Wu Minhua , Wen Limu , Wu Weizhu 
Published: November 2022

AbstractBackgroundBreast cancer (BC) is second cancer frequently occurring worldwide. Circular RNA hsa_circ_0000129 (circ_0000129) exerts a tumor‐promoting effect in BC. Nevertheless, the molecular mechanisms mediated by the upregulation of circ_0000129 during BC progression are not well understood.MethodsForty‐five BC patients were recruited for the research. Changes in circ_0000129 levels were detected with quantitative reverse transcription‐polymerase chain reaction. Cell proliferation, apoptosis, migration, invasion, and angiopoiesis were determined by cell counting, 5‐ethynyl‐2′‐deoxyuridine (EdU), flow cytometry, transwell, and tube formation assays. Protein levels were detected by western blot analysis. The regulatory mechanism of circ_0000129 was predicted by bioinformatics analysis and validated by dual‐luciferase reporter and RNA immunoprecipitation assays. In vivo experiments were carried out to verify the function of circ_0000129.ResultsCirc_0000129 was overexpressed in BC samples and cell lines. Functionally, circ_0000129 silencing reduced cell proliferation, migration, invasion, and promoted cell apoptosis, as well as induced HUVEC angiopoiesis in vitro. Furthermore, circ_0000129 knockdown decreased BC cell growth in mouse xenograft models. Mechanically, circ_0000129 interacted with miR‐485‐3p to mediate the inhibiting effect of miR‐485‐3p on SPIN1. Silenced miR‐485‐3p expression weakened the inhibiting effect of circ_0000129 knockdown on BC cell malignant behaviors. Also, forced SPIN1 expression weakened miR‐485‐3p upregulation mediated effects on BC cell malignant behaviors.ConclusionCirc_0000129 acted as a miR‐485‐3p sponge molecular to mediate expression, thus promoting BC progression.
Crosstalk of moderate ROS and PARP‐1 contributes to sustainable proliferation of conditionally reprogrammed keratinocytes
Authors: Liu Linhua , Mondal Abdul M. , Liu Xuefeng 
Published: November 2022

AbstractConditionally reprogrammed cell (CRC) technique is a promising model for biomedical and toxicological research. In the present study, our data first demonstrated an increased level of PARP‐1 in conditionally reprogrammed human foreskin keratinocytes (CR‐HFKs). We then found that PARP inhibitor ABT‐888 (ABT), reactive oxygen species (ROS) scavenger N‐acetyl‐l‐cysteine (NAC), or combination (ABT + NAC) were able to inhibit cell proliferation, ROS, PARP‐1, and ROS related protein, NRF2, and NOX1. Interestingly, knockdown of endogenous PARP‐1 significantly inhibited cell proliferation, indicating that the increased PARP‐1 expression was critical for CR. Importantly, we found that a moderate level of ROS contributed the cell proliferation and increased PARP‐1 since knockdown of PARP‐1 also inhibited the ROS. The similar inhibition of cell proliferation, ROS, and expression of PARP‐1 and NRF2 proteins was observed when CR‐HFKs were treated with hydroquinone (HQ), a key component from skin‐lightening products. Moreover, the treatment of HQ plus treatment of ABT, NAC, or combination can further inhibit cell proliferation, ROS, expression of PARP‐1, and NRF2 proteins. PARP‐1 knockdown inhibited the population doubling (PDL) and treatment of HQ inhibited the PDL further, as well as the change of ROS. Finally, we discovered that pathways including cyclin D1, NRF2, Rb and pRb, CHK2, and p53, were involved in cell proliferation inhibition with HQ. Taken together, our findings demonstrated that crosstalk between ROS and PARP‐1 involves in the cell proliferation in CR‐HFKs, and that inhibition of CR‐HFK proliferation with HQ is through modulating G1 cell cycle arrest.
Antiphospholipid antibodies in autoimmune thyroid diseases
Authors: Mankaï Amani , Melayah Sarra , Bousetta Syrine , Ghozzi Mariem , Yacoub‐Jemni Saloua , Ghedira Ibtissem 
Published: November 2022

AbstractBackgroundAntiphospholipid (aPL) antibodies have been reported in several autoimmune diseases. The aim of this study was to evaluate the frequency of aPL (anti‐cardiolipin antibodies (aCL) and anti‐β2 glycoprotein I antibodies (aβ2GPI)) in patients with autoimmune thyroid diseases (AITD).MethodsOne hundred and ninety‐five patients with AITD (139 Hashimoto's thyroiditis (HT) patients and 56 Graves' disease (GD) patients) and 90 healthy blood donors (HBD) were studied. IgG, IgA and IgM aCL and aβ2GPI were determined by ELISA.ResultsOne hundred fifty‐four AITD patients were women and 41 were men. Fifty‐six healthy subjects were women and 34 were men. The median age of patients and the control group was 45 and 38.5 years, respectively. The frequency of aPL was significantly higher in patients with AITD and in patients with HT than in HBD (33.3% vs 11.1%, p < 10−3 and 38.1% vs 11.1%, p < 10−3). The frequency of aPL in GD was significantly lower than in HT (21.4% vs 38.1%, p = 0.025). In patients with HT, aβ2GPI (34.5%) was significantly more frequent than aCL (13.6%) (p < 10−3). The frequency of aβ2GPI was significantly higher in patients with HT than in healthy population (34.5% vs 11.1%, p < 10−3). In HT patients, IgA isotype of aβ2GPI was significantly more common than in HBD and in GD patients (27.3% vs 7.8%, p < 10−3 and 27.3% vs 12.5%, p = 0.02, respectively).Conclusionaβ2GPI and not aCL were frequent in AITD. IgA was the predominant isotype of aβ2GPI. aβ2GPI‐IgA was more frequent in HT than in GD.