bluuebunny/crossref_metadata_2025_split huggingface dataset:
Internet Pornography Use Among Medical Students in India: Extent and Effect
Authors: Kumar Vipin , Sachdeva Ankur , Khullar Shilpa , Ali Enub , Abbas S. Zafar 
Published: January 2022

Background: Medical students are more susceptible to stress associated with long working hours, arduous studies, and living alone away from the home, which may affect their psychological well-being. They may indulge in the use of internet and pornography to ameliorate their stress. Internet is one of the main sources of pornographic content as it is easily available, affordable, and accessible. Objective: The current study seeks to quantitatively estimate the extent of internet addiction and pornography use among medical students in India, and its effect on their general well-being. Methods: We conducted a cross-sectional study in a medical college in North India involving undergraduate (including interns) and postgraduate medical students. Those using internet for porn use were assessed using a semi-structured sociodemographic questionnaire, General Health Questionnaire 30, WHO Quality of Life Index (WHOQOL)-BREF, and Internet Addiction Test (IAT). Results: A total of 393 medical students who agreed on their pornography internet usage were recruited for the study. Problematic pornography internet usage (moderate and 0severe IAT scores) was seen in 11% (80) of the participants. Male and married students were more likely to be problematic users. The mean duration of pornography use was 8.02±6.32 h/week. The time spent on pornography use positively correlated with IAT and General Health Questionnaire scores, whereas it negatively correlated with WHOQOL-BREF scores. Conclusions: Internet pornography use is significantly prevalent amongst medical students in India. As the duration of pornography use increases, general health measures and quality of life decrease, which may impact their well-being and academic performance.
Competency-Based Assessment Methods in Adult Cardiac Sonography: Students’ Perceptions and Clinical Evaluation
Authors: Dunstatter Douglas 
Published: January 2022

Objective: This research examined the effects of multiple combined competency-based methods on sonography students’ perceptions of adult echocardiography training components. In addition, clinical preceptor evaluation scores were compared with faculty objective structured clinical examination (OSCE) scores. Materials and Methods: A quasi-experimental nonequivalent group research design was used to evaluate students enrolled in an adult cardiac Commission on Accreditation of Allied Health Education Programs (CAAHEP) accredited curriculum. Students’ perceptions pre and post multiple competency-based methods (formative assessment, OSCE, & simulation) intervention were recorded via course evaluations. Questions were analyzed individually using descriptive statistics and the Bonferroni correction. Students’ clinical evaluation and OSCE scores were analyzed using Spearman’s rank correlation. Results: The majority of students’ perceptions pre- and postintervention of multiple competency-based assessments demonstrated significant differences, or they primarily agreed that their echocardiography knowledge or skill set was enhanced. There was weak correlation between students’ clinical competency evaluation scores and OSCE scores—post implementation of multiple competency-based assessments, rs(37) = −.01, P = .93. Conclusion: These results suggest further evaluation of the credentialing process’s clinical assessment to ensure clinical competency.
how_are_we_stranded_here: quick determination of RNA-Seq strandedness
Authors: Signal Brandon , Kahlke Tim 
Published: January 2022

Abstract
Background
Quality control checks are the first step in RNA-Sequencing analysis, which enable the identification of common issues that occur in the sequenced reads. Checks for sequence quality, contamination, and complexity are commonplace, and allow users to implement steps downstream which can account for these issues. Strand-specificity of reads is frequently overlooked and is often unavailable even in published data, yet when unknown or incorrectly specified can have detrimental effects on the reproducibility and accuracy of downstream analyses.

Results
To address these issues, we developed how_are_we_stranded_here, a Python library that helps to quickly infer strandedness of paired-end RNA-Sequencing data. Testing on both simulated and real RNA-Sequencing reads showed that it correctly measures strandedness, and measures outside the normal range may indicate sample contamination.

Conclusions
how_are_we_stranded_here is fast and user friendly, making it easy to implement in quality control pipelines prior to analysing RNA-Sequencing data. how_are_we_stranded_here is freely available at https://github.com/betsig/how_are_we_stranded_here.
STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis
Authors: Liang Tuo , Chen Jiarui , Xu GuoYong , Zhang Zide , Xue Jiang , Zeng Haopeng , Jiang Jie , Chen Tianyou , Qin Zhaojie , Li Hao , Ye Zhen , Nie Yunfeng , Zhan Xinli , Liu Chong 
Published: January 2022

Abstract
Objective
This study was aimed to identify the biomarkers for diagnosis and reveal the immune microenvironment changes in ankylosing spondylitis (AS).

Methods
GSE73754 was downloaded for the co-expression network construction and immune cell analyses. Flow cytometric analysis was performed to validate the results of bioinformatics analysis. Gene set enrichment analysis (GSEA) was performed to investigate the potential biological characteristic between different phenotypes. Pearson correlation analysis between the hub genes and the xCell score of immune cell types was performed.

Results
Signal transducer and activator of transcription 3 (STAT3) and Spi-1 proto-oncogene (SPI1) was identified as the hub genes in the datasets GSE73754. And the t-test showed that the expression level of STAT3 and SPI1 in the GSE73754 was significantly higher in AS and human leukocyte antigen (HLA)-B27(+) groups. Flow cytometric analysis showed that natural killer T cells (NKT) cells were upregulated, while Th1 cells were down-regulated in AS, which was consistent with the results obtained from bioinformatics analysis. STAT3 and SPI1 was correlated with the NKT cells and Th1 cells.

Conclusion
STAT3 and SPI1 may be a key cytokine receptor in disease progression in AS.
Interspecific complementation-restoration of phenotype in Arabidopsis cuc2cuc3 mutant by sugarcane CUC2 gene
Authors: Aslam Mohammad , She Zeyuan , Jakada Bello Hassan , Fakher Beenish , Greaves Joseph G. , Yan Maokai , Chen Yingzhi , Zheng Ping , Cheng Yan , Qin Yuan 
Published: January 2022

Abstract
Background
In plants, a critical balance between differentiation and proliferation of stem cells at the shoot apical meristem zone is essential for proper growth. The spatiotemporal regulation of some crucial genes dictates the formation of a boundary within and around budding organs. The boundary plays a pivotal role in distinguishing one tissue type from another and provides a defined shape to the organs at their developed stage. NAM/CUC subfamily of the NAC transcription factors control the boundary formation during meristematic development.

Results
Here, we have identified the CUP-SHAPED COTYLEDON (CUC) genes in sugarcane and named SsCUC2 (for the orthologous gene of CUC1 and CUC2) and SsCUC3. The phylogenetic reconstruction showed that SsCUCs occupy the CUC2 and CUC3 clade together with monocots, whereas eudicot CUC2 and CUC3 settled separately in the different clade. The structural analysis of CUC genes showed that most of the CUC3 genes were accompanied by an intron gain during eudicot divergence. Besides, the study of SsCUCs expression in the RNA-seq obtained during different stages of ovule development revealed that SsCUCs express in developing young tissues, and the expression of SsCUC2 is regulated by miR164. We also demonstrate that SsCUC2 (a monocot) could complement the cuc2cuc3 mutant phenotype of Arabidopsis (eudicot).

Conclusions
This study further supports that CUC2 has diverged in CUC1 and CUC2 during the evolution of monocots and eudicots from ancestral plants. The functional analysis of CUC expression patterns during sugarcane ovule development and ectopic expression of SsCUC2 in Arabidopsis showed that SsCUC2 has a conserved role in boundary formation. Overall, these findings improve our understanding of the functions of sugarcane CUC genes. Our results reveal the crucial functional role of CUC genes in sugarcane.
Propensity-matched analysis of the efficacy of olanexidine gluconate versus chlorhexidine-alcohol as an antiseptic agent in thoracic esophagectomy
Authors: Fujita Takeo , Okada Naoya , Sato Takuji , Sato Kazuma , Fujiwara Hisashi , Kojima Takashi , Daiko Hiroyuki 
Published: January 2022

Abstract
Background
In the present matched-cohort study, we investigated the efficacy of olanexidine gluconate in comparison with chlorhexidine-alcohol as an antiseptic agent in thoracic esophagectomy.

Methods
A total of 372 patients with esophageal cancer who were scheduled to undergo thoracic esophagectomy between 2016 and 2018 were assigned to one of two groups based on the preoperative antiseptic agent used in thoracic esophagectomy. We investigated the incidence of surgical site infectious complications in the propensity-matched cohort.

Results
Based on the propensity score, 116 patients prepared with 1.5% olanexidine gluconate and 114 patients prepared with 1.0% chlorhexidine-alcohol as surgical skin antisepsis were selected. No significant intergroup differences were observed with respect to incisional surgical site infection (0.8% in the olanexidine group versus 0.8% in the chlorhexidine group) and deep fascial/organ space surgical site infection (1.7%/10.3% in the olanexidine group versus 3.5%/15.7% in the chlorhexidine group, p = 0.39/p = 0.03). Notably, the respective incidences of surgical site infection except anastomotic leakage were 1.7% and 7.0% in the olanexidine and chlorhexidine groups (p = 0.04).

Conclusions
Olanexidine gluconate was well tolerated and significantly reduced incidence of surgical site infection except anastomotic leakage in comparison with chlorhexidine-alcohol as an antiseptic agent in thoracic esophagectomy with three-field lymph node dissection.
Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population
Authors: Alkhatib Rami , Hawamdeh Razan , Al-Eitan Laith , Abdo Nour , Obeidat Fadi , Al-Bataineh Mohamed , Aman Hatem 
Published: January 2022

Abstract
Background
This study aimed to investigate the genetic association of specific Single Nucleotide Polymorphisms (SNPs) within the muscle segment homeobox gene 1 (MSX1) with susceptibility to the peg-shaped teeth in 36 Jordanian Arab families and case–control samples in the Jordanian Arab population.

Methods
This cohort involved 108 individuals (36 trios families), which were used for family-based genetic study. Additionally, 56 patients and 57 controls were used for case–control study. Genomic DNA samples from both families and case–control were extracted according to distinguished processes. Then, polymerase chain reaction technique (PCR) was conducted using specific primers for the axons of the MSX1. Moreover, DNA sequencing genotyping method analysis of SNPs was used to detect specified SNPs in the MSX1 linked with peg-shaped teeth. Hardy–Weinberg Equilibrium and Chi-square were used to evaluate the data quality and the presence of any genotypic error. In addition, Transmission Disequilibrium Test (TDT) was used identify family-based association in which trios of parents and proband are used.

Results
The results of this study showed fourteen polymorphic sites in this gene, eight of them (rs121913129, rs104893852, rs104893853, rs121913130, rs104893850, rs1095, rs3775261, and rs1042484) were none-polymorphic. Meanwhile, the minor allele frequencies of the rest of the SNPs were polymorphic (rs8670, rs12532, rs3821949, rs4464513, rs1907998, and rs6446693). However, none of these SNPs were associated with peg-shaped teeth. Moreover, the haplotype genetic analysis revealed that there was no genetic association with peg-shaped teeth disorder susceptibility (P > 0.05) in the Jordanian families of Arab descent.

Conclusions
The present findings can be used in estimation of prevalence of peg-shaped teeth in the Jordanian population. However, our findings revealed that there is no evidence that the MSX1 polymorphisms had a crucial role in the peg-shaped teeth phenomenon, emphasizing that other genes might have this role. These findings are beneficial for clinicians to comprehensively understand the molecular aspects of teeth abnormalities.
Is respectful care provided by community health workers associated with infant feeding practices? A cross sectional analysis from India
Authors: Diamond-Smith Nadia , Gopalakrishnan Lakshmi , Walker Dilys , Fernald Lia , Menon Purnima , Patil Sumeet 
Published: January 2022

AbstractObjectivesBreastfeeding and complementary feeding practices in India do not meet recommendations. Community health care workers (CHWs) are often the primary source of information for pregnant and postpartum women about Infant and Young Child Feeding (IYCF) practices. While existing research has evaluated the effectiveness of content and delivery of information through CHWs, little is known about the quality of the interpersonal communication (respectful care). We analyzed the effect of respectful interactions on recommended IYCF practices.MethodsWe use data from evaluation of an at-scale mHealth intervention in India that serves as a job aid to the CHWs (n = 3266 mothers of children < 12 m from 841 villages in 2 Indian states). The binary indicator variable for respectful care is constructed using a set of 7 questions related to trust, respect, friendliness during these interactions. The binary outcomes variables are exclusive breastfeeding, timely introduction of complimentary feeding, and minimum diet diversity for infants. We also explore if most of the pathway from respectful care to improved behaviors is through better recall of messages (mediation analysis). All models controlled for socio-economic-demographic characteristics and number of interactions with the CHW.ResultsAbout half of women reported positive, respectful interactions with CHWs. Interactions that are more respectful were associated with better recall of appropriate health messages. Interactions that are more respectful were associated with a greater likelihood of adopting all child-feeding behaviors except timely initiation of breastfeeding. After including recall in the model, the effect of respectful interactions alone reduced.ConclusionsRespectful care from CHWs appears to be significantly associated with some behaviors around infant feeding, with the primary pathway being through better recall of messages. Focusing on improving social and soft skills of CHWs that can translate into better CHW-beneficiary interactions can pay rich dividends.FundingThis study is funded by Grant No. OPP1158231 from Bill and Melinda Gates Foundation. Trial registration number:https://doi.org/10.1186/ISRCTN83902145
Dysbiotic microbes and how to find them: a review of microbiome profiling in prostate cancer
Authors: Salachan Paul Vinu , Sørensen Karina Dalsgaard 
Published: January 2022

AbstractThe role of the microbiota in human health and disease is well established, including its effects on several cancer types. However, the role of microbial dysbiosis in prostate cancer development, progression, and response to treatment is less well understood. This knowledge gap could perhaps be implicated in the lack of better risk stratification and prognostic tools that incorporate risk factors such as bacterial infections and inflammatory signatures. With over a decade’s research investigating associations between microbiome and prostate carcinogenesis, we are ever closer to finding the crucial biological link between the two. Yet, definitive answers remain elusive, calling for continued research into this field. In this review, we outline the three frequently used NGS based analysis methodologies that are used for microbiome profiling, thereby serving as a quick guide for future microbiome research. We next provide a detailed overview of the current knowledge of the role of the human microbiome in prostate cancer development, progression, and treatment response. Finally, we describe proposed mechanisms of host-microbe interactions that could lead to prostate cancer development, progression or treatment response.
Comparison of flexion relaxation phenomenon between female yogis and matched non-athlete group
Authors: Ramezani Marzyeh , Kordi Yoosefinejad Amin , Motealleh Alireza , Ghofrani-Jahromi Mohsen 
Published: January 2022

Abstract
Background
Trunk flexion is a common exercise during daily activities. Flexion relaxation phenomenon (FRP) occurs during forward bending in which there is a sudden silence of erector spinae (ES) muscles. The pattern of forward bending differs in yoga practitioners. This learned pattern probably predisposes yogis to injuries. The hypothesis of this study was that FRP differs in yogis in comparison to non-yogis individuals.

Methods
This observational cross-sectional study was performed on 60 women assigned into yogis and non-athlete groups. Each participant was asked to bend forward and then return to the initial position. ES activity was recorded at L3 level, 4 cm from mid line during the trial. Trunk inclination and lumbar flexion angles were calculated at FRP onset and cessation moments.

Results
The FRP occurred in 80% of yoga practitioners in comparison to 96.7% in the control group. Trunk inclination angle was significantly greater at FRP initiation in yogis compared to control group. Lumbar flexion angle was not different between the groups.

Conclusions
It is concluded that the altered pattern of forward bending observed in yogis may change patterns of ES muscles activity if it becomes part of a person's daily lifestyle which might predispose these muscles to fatigue and subsequent injuries; however, further studies are warranted for clarification.
Structural diversity of marine anti-freezing proteins, properties and potential applications: a review
Authors: Ghalamara Soudabeh , Silva Sara , Brazinha Carla , Pintado Manuela 
Published: January 2022

AbstractCold-adapted organisms, such as fishes, insects, plants and bacteria produce a group of proteins known as antifreeze proteins (AFPs). The specific functions of AFPs, including thermal hysteresis (TH), ice recrystallization inhibition (IRI), dynamic ice shaping (DIS) and interaction with membranes, attracted significant interest for their incorporation into commercial products. AFPs represent their effects by lowering the water freezing point as well as preventing the growth of ice crystals and recrystallization during frozen storage. The potential of AFPs to modify ice growth results in ice crystal stabilizing over a defined temperature range and inhibiting ice recrystallization, which could minimize drip loss during thawing, improve the quality and increase the shelf-life of frozen products. Most cryopreservation studies using marine-derived AFPs have shown that the addition of AFPs can increase post-thaw viability. Nevertheless, the reduced availability of bulk proteins and the need of biotechnological techniques for industrial production, limit the possible usage in foods. Despite all these drawbacks, relatively small concentrations are enough to show activity, which suggests AFPs as potential food additives in the future. The present work aims to review the results of numerous investigations on marine-derived AFPs and discuss their structure, function, physicochemical properties, purification and potential applications.Graphical Abstract
Aerodynamic loads and bridge responses under train passage: case study of an overpass steel box-girder cable-stayed bridge
Authors: Liu Ziqi , Zhang Xun , Chen Tao , Liu Yuyi , Lu Bin 
Published: January 2022

AbstractIncreased train speeds have led to significant train-induced wind problems. This paper describes a numerical study of the aerodynamic loads produced when a train passes under a bridge and the bridge dynamic response based on a steel box-girder cable-stayed bridge. The results indicate that the wind pressure on the bottom plate and the maximum lift on the beam sections increase as the train speed increases or as the clearance under the bridge decreases. Changes in the intersection angle are found to have a greater impact on the 1/4-span and 3/4-span regions. The effects of the various influencing factors are basically the same in the construction and operation stages. As the train speed increases or the clearance under the bridge decreases, the bridge responses exhibit an upward trend. As the intersection angle decreases, the vibration displacement tends to increase, but the effects on the vibration acceleration and maximum counter-force of the temporary pier are not consistent. Compared with the construction stage, the dynamic responses of the main girder are smaller in the operation stage due to the larger overall rigidity and the support constraints. In the parameter ranges investigated in this study, the absolute values of maximum vertical bridge vibration displacement and acceleration decrease from 4 to 16 mm and from 85 to 400 mm/s2, respectively, in the construction stage to 1–5 mm and 42–174 mm/s2 in the operation stage. Moreover, the counter-force falling on the temporary pier after the main girder turns does not exceed the 2000-kN positive force or 300-kN negative force limits. In the operation stage, the bridge vibration responses will not affect the comfort of pedestrians.
GENDER EQUALITY AS A BASIS FOR HARMONIOUS DEVELOPMENT OF THE STATE AND SOCIETY: SOCIAL AND LEGAL ASPECT
Authors: Golovinova Yulya V. 
Published: January 2022

Within the framework of this publication, an urgent problem is considered - gender equality as a platform for full-fledged sustainable and harmonious development of the state and society in the brow. The author focuses on two aspects, sociological and legal. The article examines the phenomenon of gender equality using the achievements of gender sociology as a special sectoral sociological discipline that studies the processes of development and social interaction of male and female communities. However, the investigated issues are also cognized taking into account the achievements of the theory of law and state and the science of constitutional law. In our opinion, such a positive heuristic synthesis allows us to study the subject of the publication - the principle of gender equality. By the purpose of the work, the author understands an attempt to identify and show social and legal mechanisms that make it possible to eliminate gender asymmetry in relations that are currently demarcated by gender. The publication established that an illegitimate encroachment restricting human rights is a flagrant injustice. In this capacity, equality as a principle of law is nothing more than the ideal of a just structure of the state and society, which is characterized by the comprehensive implementation and protection of the rights and freedoms of man and citizen. Therefore, equality canonizes parity in the relationship between the individual and the state, discrimination on the part of state bodies with this interpretation is excluded.