bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anxiety and Test Form: The Differences of Test Anxiety Levels in Terms of Test Form
Authors: Wahyuni Lussy Dwiutami , Citraini Rezza , Hutomo Bijak Aditia , Gumelar Gumgum 
Published: March 2022

Education is one of the most important aspects of human life. In the education file, examination is an important part to evaluate the progress of the students. The students need to fulfill the test parameters and indicators in order to move to the higher level of class or higher degree. The common feeling while students feel anxious in facing the test is called test anxiety. There are several factors that affect test anxiety, and one of the most common sources is the test form. This study aimed to determine the differences in test anxiety levels in students in terms of multiple-choice and matchmaking tests. The population of this study was 142 students of the Faculty of Psychology Education that registered in 2018. The design of this study was a quasi-experimental research with two-group pretest-posttest design. The instrument used in this study was the Reaction to Test scale by Irwin G. Sarason. The results showed that the multiple-choice test anxiety level was higher than the matching test, although the difference was small. The multiple-choice test anxiety level was (M = 2.17) and matchmaking (M = 2.07).
Stress Ulcer Prophylaxis for Critical Asthma
Authors: Roberts Alexa Rae , Roddy Meghan , Wilsey Michael John , McKinley Scott Daniel , Sanchez-Teppa Beatriz , Sochet Anthony Alexander 
Published: March 2022

BACKGROUND
Children hospitalized for critical asthma (CA) in the pediatric ICU (PICU) are commonly prescribed stress ulcer prophylaxis (SUP) to mitigate risk of gastrointestinal (GI) bleeding. We sought to describe trends for SUP prescribing and explore for differences in rates of GI bleeding, gastritis, and SUP-related complications for those with and without SUP exposure.

METHODS
We performed a retrospective, multicenter cohort study using the Pediatric Hospital Information System registry among 42 children’s hospitals from 2010 to 2019 including children 3 to 17 years of age admitted to the PICU for CA. Primary outcomes were chronologic and regional variation in SUP prescribing assessed by Joinpoint regression and Pearson’s correlation. Rates of GI bleeding, gastritis, enteric ulceration, and SUP-related complications (C. difficile colitis, necrotizing enterocolitis, and thrombocytopenia) were compared for those with and without SUP exposure.

RESULTS
Of 30 177 children studied, 10 387 (34.4%) received SUP. No episodes of GI bleeding were recorded. One subject developed gastric ulceration and 32 (0.1%) gastritis. Linear trends for SUP were observed with rates increasing from 25.5% in 2010 to 42.1% in 2019 (+1.9% annually). Prescribing varied by institution (range: 5.5% to 97.2%) without correlation to admission volumes. Extremely rare rates of SUP-related complications were noted.

CONCLUSIONS
Although children hospitalized for CA routinely receive SUP, no episodes of GI bleeding were noted over a 10-year period. SUP solely for corticosteroid exposure may be unwarranted. We advocate for a targeted approach to SUP considering alternative risk factors for GI bleeding.
Reading trajectories in children with language disorders
Authors: Nelson Ryan , Damico Jack S. , Damico Holly L. , Lynch Karen , Abendroth Kathleen J. , Weill Christine , Arrington Laura E. , Percle Amanda 
Published: March 2022

Background: The literature on typically developing children's literacy acquisition provides a conventional model of development; however, little is available regarding how children with communicative disorders develop literacy abilities over time. This study describes the temporal trajectories of reading development in three children with diagnoses of language disorders and histories of reading difficulty. Method: A qualitative research design employing ethnographic data collection and analysis procedures was used. One male (age 8 years 9 months) and two females (ages 7 years 8 months and 7 years 11 months) identified as having impaired language and a history of reading difficulty were followed for 10 months as they engaged in literacy instruction. Results: Each child demonstrated a unique trajectory of literacy improvement. Trajectories of development were observed across the complexity of the materials read, as well as across variables reflective of comprehension. Variables included formal miscue analysis data, retelling of stories read, and text complexity. Discussion/conclusion: This study suggests that each language-impaired individual should be considered separately in how they progress and in how progression is manifested. If norm-referenced, standardized evaluation methods had been applied, or if only one sampling period had been used, the changes and progress noted in each of these participants may have been missed.
Helping aphasic patients accomplish greeting exchanges
Authors: Wilainuch Pairote 
Published: March 2022

Background: This study explores greeting exchanges in stroke care, in particular the use of the ‘wai’ gesture. Method: Seventeen patients with Broca's aphasia, some family members, six nurses from district public health centers, and four nurses from a district hospital in northern Thailand were given written consent forms for participation. Thirty counseling sessions were video-recorded in patients’ homes and analyzed using conversation analysis. Direct observation and in-depth interview were also used for supplementary data collection. Results: These showed a patient's daughter helping her to accomplish a greeting. She was encouraged to use one hand to raise the other hand up. Another patient was only able to raise one hand to conduct the normal ‘wai,’ a potential cause of embarrassment for the patient, as the greeting is always formed by putting the two palms of the hands together. The nurse encouraged him to perform the greeting using one hand through different questions and statements. Discussion and conclusion: While the ‘wai’ gesture and the spoken greeting ‘sawatdi’ used for social functions plays an important role in stroke counseling, the nursing guidelines in the Barthel Index excluded them in the section on non-verbal communication assessment. This article suggests that they should be taken into account, in order to improve the nursing guidelines to fit the Thai context.
DGAT2 Inhibition Potentiates Lipid Droplet Formation To Reduce Cytotoxicity in APOL1 Kidney Risk Variants
Authors: Chun Justin , Riella Cristian V. , Chung Hyunjae , Shah Shrijal S. , Wang Minxian , Magraner Jose M. , Ribas Guilherme T. , Ribas Hennrique T. , Zhang Jia-Yue , Alper Seth. L. , Friedman David J. , Pollak Martin R. 
Published: March 2022

Significance Statement
Variants G1 and G2 of the gene encoding apolipoprotein L1 (APOL1) are associated with increased risk of kidney disease in certain populations. In previous work, the authors demonstrated that recruitment of these variants from the endoplasmic reticulum to lipid droplets is associated with reduced cytotoxicity in podocytes. In this study, they confirm differences in lipid droplet distribution between isogenic human kidney organoids expressing wild-type APOL1 (G0) or risk variant APOL1 (G2), which are affected by the levels of APOL1 expression. They also demonstrate that inhibition of diacylglycerol O-acyltransferase 2 (DGAT2), a key enzyme in triglyceride biosynthesis, upregulates genes involved in lipid droplet formation. DGAT2 inhibitors may thus represent a potential therapeutic approach to reduce the cytotoxic effects of APOL1 risk variants that contribute to APOL1 nephropathy.

Background
Two variants in the gene encoding apolipoprotein L1 (APOL1) that are highly associated with African ancestry are major contributors to the large racial disparity in rates of human kidney disease. We previously demonstrated that recruitment of APOL1 risk variants G1 and G2 from the endoplasmic reticulum to lipid droplets leads to reduced APOL1-mediated cytotoxicity in human podocytes.

Methods
We used CRISPR-Cas9 gene editing of induced pluripotent stem cells to develop human-derived APOL1G0/G0 and APOL1G2/G2 kidney organoids on an isogenic background, and performed bulk RNA sequencing of organoids before and after treatment with IFN-γ. We examined the number and distribution of lipid droplets in response to treatment with inhibitors of diacylglycerol O-acyltransferases 1 and 2 (DGAT1 and DGAT2) in kidney cells and organoids.

Results
APOL1 was highly upregulated in response to IFN-γ in human kidney organoids, with greater increases in organoids of high-risk G1 and G2 genotypes compared with wild-type (G0) organoids. RNA sequencing of organoids revealed that high-risk APOL1G2/G2 organoids exhibited downregulation of a number of genes involved in lipogenesis and lipid droplet biogenesis, as well as upregulation of genes involved in fatty acid oxidation. There were fewer lipid droplets in unstimulated high-risk APOL1G2/G2 kidney organoids than in wild-type APOL1G0/G0 organoids. Whereas DGAT1 inhibition reduced kidney organoid lipid droplet number, DGAT2 inhibition unexpectedly increased organoid lipid droplet number. DGAT2 inhibition promoted the recruitment of APOL1 to lipid droplets, with associated reduction in cytotoxicity.

Conclusions
Lipogenesis and lipid droplet formation are important modulators of APOL1-associated cytotoxicity. Inhibition of DGAT2 may offer a potential therapeutic strategy to attenuate cytotoxic effects of APOL1 risk variants.
Digital measurement of nasal resonance in the adult Polish population
Authors: Krasnodębska Paulina , Nikiel Katarzyna , Będziński Wojciech , Milewska Marzena , Szkiełkowska Agata , Miaśkiewicz Beata 
Published: March 2022

WstępNosowanie to subiektywne odczucie nieprawidłowej wartości rezonansu nosowego w mowie. Miarą obiektywną nosowania jest nosowość wyrażająca stosunek rezonansu nosowego do łącznej wartości rezonansu nosowego i ustnego.CelCelem pracy był pomiar na nazometrze cyfrowym udziału rezonansu nosowego podczas artykulacji w populacji zdrowych dorosłych Polaków. Kolejnym celem było sprawdzenie, czy opracowane w przeszłości testy artykulacyjne do oceny rezonansu nosowego mogą być stosowane na nowoczesnych urządzeniach cyfrowych.Materiał i metodyMateriał w pracy stanowiło 45 osób z prawidłowo rozwiniętą mową, prawidłowym słuchem oraz brakiem odchyleń w badaniu otolaryngologiczno-foniatrycznym. Pacjentów badano testami językowymi opracowanymi przez Gąsiorka. Użyto testu I, III i VII.WynikiŚrednie wyniki nosowości (śrN) podczas pierwszego testu zawierającego samogłoski (śrNI) w całej grupie badanej wyniosły 18,6% (SD 8,8%); testu zawierającego zdania (śrNIII) – 14,7% (SD 4,1%); podczas czytania tekstu śrNVII – 11,9% (SD 3,8%). Analiza statystyczna nie wykazała istotnych różnic pomiędzy płcią a wynikami uzyskanymi w każdym z testów.WnioskiWykazano, że opracowane i wykorzystywane do tej pory testy językowe można zastosować na nowoczesnych urządzeniach cyfrowych. Autorzy rekomendują zastosowanie zróżnicowanych testów językowych dla dzieci i osób dorosłych.
Alcohol exacerbated nitroxidative stress in brain of diabetic rats: An ameliorative role of green tea
Authors: Varadacharyulu NCh , Swarnalatha K , Fareeda Begum S , Mohan Chandra M , Ramaiah Ch. Venkata 
Published: March 2022

Alcohol abuse and diabetes exist in many populations as comorbities. Alcoholism and diabetes can induce wide spectrum of effects on central nervous system. This study focussed on, the impact of nitroxidative stress in alcohol treated diabetic rats and to evaluate the possible protective effect of aqueous extract of green tea (GTE). Male albino Wistar rats aged of 8 weeks were made diabetic with steptozotocin (STZ) and treated with at 5 g/kg b.w/day (20% v/v) alcohol for sixty days. Alongside enhanced reactive oxygen species (ROS), reactive nitrogen species (RNS) levels and neuronal nitric oxide synthase (nNOS) expression were found to be increased and activities of antioxidant enzymes were decreased in the brain of alcohol treated diabetic rats. When the alcohol was administered to the diabetic rats, it is difficult to know whether the observed changes are independent or overlapping effects. Increased expression of mRNAs of Bcl, Bax, and TNF-α, IL-6, Caspase-3, and COX2 genes suggest that they may have functional significance about alcohol intoxication. This study provided information that, green tea catechin, (-)-epigallocatechin-3-O-gallate (EGCG) has a therapeutic effect and thus ameliorate the effect of alcohol in diabetic condition.
Nano-chitosan Spray as a Preservative and Food Security of Fishery Products in The Middle of the Covid-19 Pandemic
Authors: Saputra Dion , Ula Ferra Robiatul , Fadhila Ajeng Budiarahma Nur , Sijabat Yosi Yananda , Romadoni Abista Ahmad , Windarto Seto 
Published: March 2022

Highlight ResearchThe author mentioned 2 highlights from their results research Nano-chitosan has the best susceptibility to bacterial strains of E. coli, S. enteritidis, L. monocytogenes, B. cereus and S. aureus.Nano-chitosan showed higher degree of inhibition than that done by chitosan. AbstractThe COVID-19 pandemic has impacted the fisheries sector, a decline in exports and fishermen's income caused by the disconnection of the marketing chain due to lockdown implementation in several export destination countries. Fish is a source of protein and as perishable goods, it experiences quality damage due to spoilage, commonly caused by hampered distribution of catches. Natural preservatives are needed to preserve catch so it won’t get spoiled and can be accepted by consumers in suitable conditions for consumption. This study aims to evaluate the particle size of nano-chitosan and determine the effectiveness of nano-chitosan spray with different concentrations as a natural preservative in caught fish. This research method begins with making chitosan through 3 stages: demineralization, deproteination, and deacetylation. Chitosan was made into nano-chitosan with various concentrations of 3:1, 4:1, and 5:1 using ionic gelation. Nano-chitosan underwent several tests, including PSA, antibacterial activity by disc diffusion, organoleptic, and the effectiveness of preservatives. Antibacterial activity of nano-chitosan was able to inhibit Bacillus subtilis at three different concentrations, the potential to inhibit Escherichia coli was optimal at 5:1 treatment. The effectiveness of nano-chitosan preservative bacteria inhibition at three different concentrations proved to be sufficient to be used to extend shelf life and ensure the safety and quality of fishery products. The best concentration of nano-chitosan was 3:1 treatment. Nano-chitosan spray from crab shell waste has good antibacterial activity and preservative effectiveness. It could serve as an antibacterial agent and natural preservative for fishery products during the COVID-19 pandemic.
Morphometric and Meristic Characterization of Hairtails Trichiurus lepturus Linnaeus, 1758 (Scombriformes: Trichiuridae) from the Northern Coast of Java, Indonesia
Authors: Aryantojati Adhimas Febri , Murwantoko Murwatoko , Setyobudi Eko 
Published: March 2022

Highlight ResearchThe most significant morphological variation was the snout length and caudal peduncleT. lepturus from the coast of Pati and Cirebon have a high morphometric character resemblance and possibly are members of the same populationT. lepturus in Demak waters formed a separate population group from T. lepturus from the coast of Pati and Cirebon, however still in the same species AbstractHairtails (Trichiurus spp.) are economically important fisheries resources and have become an export commodity. In Indonesia, there are several hairtail species; however, each species distribution is not yet certainly known. This study aimed to identify and determine the morphometric and meristic character of the hairtail from the northern coast of Java, Indonesia. Fish specimens were obtained from fishermen catches in the north coast of Pati and Demak (Central Java) and the northern coast Cirebon (West Java), which captures the fish in less than one-week fishing operation. Fish samples were grouped based on morphological similarities identified in previously published works. After that, 22 morphometric and four meristic characters were measured and counted. Principal Component Analysis and Discriminant Analysis were used to analyze morphometric data, while the meristic data were compared to the available published works. The hairtail caught at the northern coast of Java was long, flat, silvery in color, and had a sagittal crest, with the fin formula being D.III.125-134, P.I.10, and A.I.98-104. According to the morphological and meristic characters, the hairtail caught at Java’s northern coast was identified as Trichiurus lepturus. The most significant morphological variation was the snout length and caudal peduncle. The PCA and scattergram analyses showed that T. lepturus from the coast of Pati and Cirebon have a high morphometric character resemblance and possibly are members of the same population. T. lepturus in Demak waters formed a separate population group from T. lepturus from the coast of Pati and Cirebon, however still in the same species.
The Effect of Flushing Premating with Spirulina Platensis Supplementation on Ewes Postpartum Estrus
Authors: Sarvinda Diahanvika Tri , Bintara Sigit , Budisatria I Gede Suparta , Kustantinah Kustantinah , Baliarti Endang 
Published: February 2022

Lactating ewes require high nutrients for basic life requirements and milk production. If not fulfilled, it can have an impact on Negative Energy Balance (NEB) that reduces body weight, Body Condition Score (BCS), and extend the appearance of Postpartum Estrus (PPE). Premating flushing feed is an effort to improve ewe nutrients by adding high nutrition for preparation before mating so that after lambing and suckling, the ewe immediately estrus. This research aimed to evaluate the performance of ewes through premating flushing feeding. The research was conducted at Mendo Galak Farm, Sleman, Yogyakarta. Twenty ewes 2-3 years old with BCS 2-3 divided into two groups; the group with flushing treatment consisted of dried water spinach (Ipomoea reptans poir), concentrate feed with Spirulina sp. (14,92% crude protein, 60,28% total digestible nutrients), and the control group (PS) without Spirulina sp. (crude protein 11,82%, total digestible nutrients 53,20%). Flushing feed was given after a month postpartum as much as 3% dry matter of body weight. The recorded parameter was daily feed consumption, monthly body weight, BCS, and postpartum estrus. The data obtained were tested by an independent T-test with Statistical Product and Service Solution (SPPS ver. 22). The results showed the consumption and digestibility of CP, TDN, and ewe's ADG had a significant difference (P<0.05). Postpartum estrus (PPE) of flushed ewes had no significant difference (P>0.05), 73.90±11.55 vs. 77.60±14.65 days, respectively. The conclusion was that flushing premating treatment with the addition of Spirulina platensis increased the nutrient intake and digestibility of CP, TDN, and ADG but had not shortened on postpartum estrus of lactating ewes.
The Quality of Buffalo Sperm Following Preservation Using Different Diluents and Sperm Concentrations
Authors: Yendraliza Yendraliza , Ridho Muhammad , Rodiallah Muhamad , Zumarni Zumarni 
Published: February 2022

Artificial Insemination (AI) success depends on the quality of the frozen semen. The quality of the frozen semen of swamp buffalo in Indonesia is still low. The study was conducted to determine the quality of buffalo sperm following freezing using three different diluents and three different doses. The study used buffalo semen from the Tuah Sakato Artificial Insemination Center, Payakumbuh (n = 3). The semen collecting was carried out once a week for 10 weeks (replication). The research method used was 3x3 factorial randomized block design. The first factor was diluent (Triladyl®, Andromed® and Tris egg- yolk) and the second factor was the dose of spermatozoa (10 and 15 and 20 x 106 sperm/ml). Data were analyzed using variant analysis, while the differences between treatments were tested by Duncan Multiple Range Test. The results showed that the plasma membrane integrity of buffalo sperm was found in Andromed® diluent, while tris egg-yolk diluent gave better motility, viability, plasma membrane integrity and recovery rate at a sperm concentration of 20 x106 sperm/mL compared to triladyl® diluent and a sperm concentration of 10 and 15 x106 sperm/mL. It was concluded that andromed® diluent and tris egg-yolk gave better motility, viability, plasma membrane integrity and recovery rate at a sperm concentration of 20 x106 sperm/mL compared to triladyl® diluent and a sperm concentration of 10 and 15 x106 sperm/mL. of Buffalo of sperm abnormalities not sicnificantly by the type of diluent but are influenced by sperm concentration.
To Determine the Effect of Using a Complete Feed Containing Protected Soybean Groats in the Production of Javanese Thin-Tailed Male Sheep Carcasses
Authors: Arif Yahya Nor , Isnaniyah Nurul Fatimah , Pramono Ahmad , Riyanto Joko 
Published: February 2022

The present study aims to determine the impact of complete feed containing protected soybean meal on the production of male thin-tailed lamb carcasses. A total of 15 (23.43 ± 1.40 kg) 12 months old male Thin-Tailed Sheep (TTL) were given 3 treatments and 5 replicates in a completely randomized design. The ration consisted of complete feed and formaldehyde-protected soybean groat (PSG). The treatments includes 100% complete feed (F1), 90% complete feed + PSG 10% (F2), and 80% complete feed + PSG 20% (F3). Data were analyzed using ANOVA and differences between treatments were further tested using Duncan’s New Multiple Range Test. The results indicated that the use of PSG containing complete feed did not improve the characteristics of carcasses (weight and percentage of carcass components, half-cuts and commercial cuts of carcass, fleshing index and meat bone ratio) of male TTLs (P>0.05). However, differences were found in the rib eye muscle area. The use of complete feed containing 20% PSM (F3) resulted the highest rib eye muscle area figure compared to those of F2 and F1 (35.17 vs 27.00 and 26.33; P<0.05). Our study revealed that the use of complete feed containing 20% protected soybean groat resulted in higher rib eye muscle area by 25.14% compared to those containing no protected soybean groat. However, no differences in carcass characteristics were found in view of weight and percentage of carcass components, half cut and commercial carcass, fleshing index and meat bone ration in thin-tailed sheep.