bluuebunny/crossref_metadata_2025_split huggingface dataset:
PRMT6-CDC20 facilitates glioblastoma progression via the degradation of CDKN1B
Authors: Wang Zhaotao , Wang Ji , Xiao Zongyu , Li Peng , Wu Chunwang , Li Yan , Wang Qing , Chen Yanming , Zhou Honglong , Li Zhi , Lan Qing , Wang Yezhong 
Published: October 2022

Abstract
PRMT6, a type I arginine methyltransferase, di-methylates the arginine residues of both histones and non-histones asymmetrically. Increasing evidence indicates that PRMT6 plays a tumor mediator involved in human malignancies. Here, we aim to uncover the essential role and underlying mechanisms of PRMT6 in promoting glioblastoma (GBM) proliferation. Investigation of PRMT6 expression in glioma tissues demonstrated that PRMT6 is overexpressed, and elevated expression of PRMT6 is negatively correlated with poor prognosis in glioma/GBM patients. Silencing PRMT6 inhibited GBM cell proliferation and induced cell cycle arrest at the G0/G1 phase, while overexpressing PRMT6 had opposite results. Further, we found that PRMT6 attenuates the protein stability of CDKN1B by promoting its degradation. Subsequent mechanistic investigations showed that PRMT6 maintains the transcription of CDC20 by activating histone methylation mark (H3R2me2a), and CDC20 interacts with and destabilizes CDKN1B. Rescue experimental results confirmed that PRMT6 promotes the ubiquitinated degradation of CDKN1B and cell proliferation via CDC20. We also verified that the PRMT6 inhibitor (EPZ020411) could attenuate the proliferative effect of GBM cells. Our findings illustrate that PRMT6, an epigenetic mediator, promotes CDC20 transcription via H3R2me2a to mediate the degradation of CDKN1B to facilitate GBM progression. Targeting PRMT6-CDC20-CDKN1B axis might be a promising therapeutic strategy for GBM.
Association between lysozyme and carboxymethyl konjac glucomannan to produce dispersible coacervates for edible coating
Authors: GU Chunye , Zhang XinRu , Huang GuoQing , Geng Xin , Xiao JunXia 
Published: October 2022

Abstract
Lysozyme was subjected to coacervate with carboxymethyl konjac glucomannan (CMKGM) to check if dispersible coacervates could be formed to extend its application in edible coatings. The results indicated that complex coacervation could occur between lysozyme and CMKGM only within a narrow pH range from pH 2.5 to 6.0 and the maximum interaction was recorded in a moderate temperature of 35 °C in the absence of NaCl. The dispersion status of the lysozyme – CMKGM coacervates was closely related to the phase separation pH as well as lysozyme to CMKGM mixing ratio and conditions that led to the presence of free CMKGM in the solution favored the formation of dispersible coacervates. Rheological measurements revealed the high viscosity of CMKGM solution contributed greatly to the dispersion status of the lysozyme – CMKGM coacervates. Coacervation with CMKGM caused structural changes to lysozyme, but only slightly affected its lytic activity. Contact angle measurement revealed that the lysozyme – CMKGM coacervates were more hydrophobic than the two single polyelectrolytes. Hence, the dispersible lysozyme – CMKGM coacervates were potential in the fabrication of composite antibacterial edible coatings.
Analysis of Physical Education Classroom Teaching after Implementation of the Chinese Health Physical Education Curriculum Model: a Video-Based Assessment
Authors: Chao Liu , Hui LiXiao , HuanHuan Huang , QiuLin Wang 
Published: October 2022

Abstract
This study assessed the Chinese Health Physical Education Curriculum Model recently suggested to meet the recommended physical education curriculum reforms addressing declining physical and mental health of students in China. We used video analyses of 41 physical education classroom teaching cases with a physical education classroom teaching behavior analysis system to provide quantitative and qualitative behavioral data. We established reference ranges for classroom teaching behavior indicators, summarized classroom teaching patterns, and assessed classroom discourse and the emotional climate. Notable findings included teachers in elementary schools using closed-ended questions, predictable response, and general feedback significantly more often that teachers in senior high school, and ball sports instructors using demonstration and competition significantly more frequently than instructors in athletics. Overall, three teaching patterns were most commonly used—lecture, practice, and dialogue—with practice being dominant. Analysis of the top 50 most commonly spoken words by teachers identified five types of discourse—motivational, directive, specialized, transitional, and regulatory—with motivational words being most frequent. The classroom atmosphere was mainly positive. These findings provide evidence that the use of this curriculum model may bring positive changes to physical education classroom teaching methods in China and will inform subsequent innovative physical education classroom teaching practices.
The effect of date seed powder supplementation on glycemic markers and lipid profile in women with overweight and obesity: a randomized controlled trial
Authors: Hashemzadeh Maral , Miri Hamid Heidarian , Kazemi Asma 
Published: October 2022

Abstract
Objective: The aim of this randomized controlled trial (RCT) was to investigate the effect of date kernel on glycemic control, lipid profile, and weight in women with overweight and obesity.
Design: One hundred twenty-four women with overweight or obesity were randomly assigned to receive the date kernel supplement or placebo for 12 weeks. Serum HbA1c was measured by High-performance liquid chromatography (HPLC), and fasting plasma glucose and lipid profiles by enzyme-linked immunosorbent assay (ELISA) kits. The participants' dietary intake and physical activity were recorded at baseline.
Results: A total of 98 subjects aged 40.7 years completed the trial (48 in the date kernel group, and 50 in the placebo group). From baseline to 12 weeks, date kernel supplementation resulted in a significant decrease in HbA1c [mean (SD): -0.45 (0.36)] and FPG compared to the placebo [-0.16 (0.27)] (p <0.001). No significant change was observed in the lipid profiles of the total participants. However, date kernel consumption decreased cholesterol and TG in hypercholesterolemic and hypotriglyceridemic participants compared to the placebo.
Conclusion: Overall, 12 weeks of date kernel supplements in overweight or obese women resulted in an improvement in glycemic markers compared with the placebo. In terms of lipid profile, a significant reduction in total cholesterol and TG were observed only in hyperlipidemic subjects.
Study was registered in IRCT.ir under IRCT20211217053429N1.
Aerodynamic Design of Buses Inspired by Beluga Whales
Authors: Arabacı Seda Kırmacı , pakdemirli mehmet 
Published: October 2022

Abstract
New bus designs inspired by the perfect streamlined body shapes of beluga whales are presented. The bodies are drawn using the UNIGRAPHICS program. The drag forces and the drag coefficients of variants of the new bus models are determined both experimentally and numerically. For numerical computations, the drawings are inserted into the ANSYS CFD program for meshing. The drag forces and the drag coefficients corresponding to the various designs are then computed using the FLUENT (solver) and the CFX (result) program. The experimental determination of the drag forces and the drag coefficients are performed in a wind tunnel test. Both numerical and experimental analysis reveals that substantial reductions in drag forces and drag coefficients are possible using the beluga whale inspired buses. Due to the reduced drag, lower fuel consumptions are expected for the new designs.
Comparison of transnasal humidified rapid-insufflation ventilatory exchange and face mask ventilation on safe apneic duration in elderly minimally-toothed patients：A Randomized Controlled Tria
Authors: Wang Wan-Ling , Wang Shuai , Xu Ya-Jie , Zhang Wen-Wen , Zhang Yong , Zhang Chen , Yin Jia-Lin , Bao Hong-Guang , Wang Xiao-Liang 
Published: October 2022

Abstract
Objective: To compare the effect of THRIVE with face mask ventilation on oxygenation and safe apneic duration after induction of general anesthesia in elderly minimally-toothed patients.
Method:Single university-affiliated hospital, conducted from October 2021 to December 2021. Totally 50 patients aged ≥ 65 years with ≥ 10 missing teeth, American Society of Anesthesiology physical status I-III, Mallampati class I-II, who underwent elective surgery under general anesthesia with tracheal intubation, were randomly enrolled and assigned to a facemask group (Group M) and a THRIVE group (Group T) with a random number table. Patients in Group M were pre-oxygenated with a facemask (100% oxygen at a flow rate of 6 L/min). In Group T, patients with their mouths closed were pre-oxygenated via THRIVE (100% oxygen at a flow rate of 30 L/min). After anesthesia induction, patients in Group M were ventilated with pressure-controlled ventilation, and then the facemask was removed to stop the mask ventilation. In Group T, the patient’s mouth was kept closed, and the flow rate was adjusted to 70 L/min. Then, THRIVE was continued with an open mouth. When the safe apneic duration lasted up to 8 minutes or SpO2 decreased to 95%, observation was ended, and the patient was immediately intubated with a video laryngoscope. Tracheal intubation was successfully performed on the first attempt. The safe apneic time (SAT) (from 4 min after muscle relaxant administration to the time until SpO2 dropped to 95%) was measured.
Result: Fifty patients were included in the trail. Group T patients had a significantly longer SAT compared to Group M (P=0.000). All 25 patients in Group T reached 8 minutes with their SpO2 maintained at >95%. In Group M, 6 patients (24%) maintained their SpO2 at >95% for 8 minutes.
Conclusion: THRIVE, compared with facemask ventilation, can significantly increase the safe apneic duration, improve oxygenation, and shorten the reoxygenation time in elderly minimally-toothed patients during the induction of general anesthesia, which contributes to stable hemodynamics and safe tracheal intubation.
Fabrication of Sandwich Structure Membrane and its Performance for Photocatalytic Reduction of Cr(VI)
Authors: Ren Hai-Tao , Li Da-Shuai , Jing Meng-Zhen , Li Ting-Ting , Lou Ching-Wen , Lin Jia-Horng 
Published: October 2022

Abstract
The regenerated cellulose membrane (RC) was synthesized by dissolving cotton cellulose in NaOH/CO(NH2)2 system. The Polydopamine/Bismuth Tungstate/RC composite membrane (RCPB) with visible light catalytic activity was synthesized via loading polydopamine-modified bismuth tungstate (PDA/BWO) composite on the RC by blending method. The RCPB/PAN/RCPB sandwich structure membrane was synthesized through combining the polyacrylonitrile (PAN) nanofiber membrane and RCPB by scraping method, which could reduce aqueous Cr(VI) under visible light. Characterization analysis showed that the tensile strength, elongation at break, roughness and initial water contact angle of RCPB/PAN/RCPB were 32.1 MPa, 5.34%, 0.658 µm and 69.0°, respectively. The photoreduction percent of Cr(VI) by RCPB/PAN/RCPB could reach 99.7% within 120 min with a rate constant of 0.0869 min–1, and the photoreduction percent remained above 84.6% after four cycles. The introduction of PAN further improved the mechanical properties and recycling ability of RCPB. Meanwhile, the capture experiment revealed that the main active substance for photocatalytic reduction of Cr(VI) by RCPB/PAN/RCPB was photogenerated e-. This work provides a new idea for the treatment of Cr(VI)-containing wastewater.
High Red blood Cell Distribution Width Contributes to Heart Failure Hospitalization in Patients with Pacemaker Implantation: A Retrospective Cohort Study
Authors: Yao Hongxia , Lian Liyou , Zheng Rujie , Chen Chen 
Published: October 2022

Abstract
Background: Red blood cell distribution width (RDW) has been showed to have independent predictive ability of mortality in patients with coronary artery disease and HF. However, no relevant research is established to demonstrate the relationship between RDW and heart failure hospitalization (HFH) in patients who received pacemaker.
Methods: The baseline RDW was individually recorded at admission in patients with pacemaker implantation. Patients were assigned to high and low RDW groups based on cut-off values. Restricted cubic splines were used to model the RDW-HFH association. The curve of patients free of HFH was plotted via the Kaplan-Meier method. And the significance of association between RDW and HFH was analyzed using both crude and adjusted cox proportional hazard model with hazard ratios (HR) and 95% confidence intervals (CI).
Results: A total of 927 patients who successfully received pacemaker implantation were enrolled. Seventy and seven (8.3%) patients met the endpoint, and 61(12.8%) patients had RDW≥13.45. Analysis of the receiver operating characteristicscurve for RDW demonstrated an optimal cut-off value of 13.45. The incidence of HFH in patients with high level of RDW were higher than that in those patients with low level of RDW (P＜0.001). Moreover, despite controlling for other potential risk factors, the incidence of HFH was higher than the high RDW group (HR=2.197, 95% CI:1.638-5.196, P<0.001).
Conclusion: High RDW contributes to HFH in patients with pacemaker implantation during the long-term follow-up, suggesting that RDW can effectively predict the probability of HFH for patients with pacemaker implantation.
Development and validation of postoperative circulating tumor DNA combined with clinicopathological risk factors for recurrence prediction in patients with stages I-III colorectal cancer
Authors: Gao Zhaoya , Huang Dandan , Chen Hui , Yang Yong , An Ke , Ding Changmin , Yuan Zheping , Zhai Zhichao , Niu Pengfei , Gao Qingkun , Cai Jinping , Zeng Qingmin , Wang Yanzhao , Hong Yuming , Rong Wanshui , Huang Wensheng , Lei Fuming , Wang Xiaodong , Chen Shiqing , Zhao Xiaochen , Bai Yuezong , Gu Jin 
Published: October 2022

Abstract
Background
Circulating tumor DNA (ctDNA) detection following curative-intent surgery could directly reflect the presence of minimal residual disease, which is ultimately the cause of clinical recurrence. However, ctDNA is not postoperatively detected in ≥ 50% of patients with stage I-III colorectal cancer (CRC) who ultimately recur. Herein we sought to improve recurrence risk prediction by combining ctDNA with clinicopathological risk factors in stage I-III CRC.
Methods
Two independent cohorts, both consisting of early-stage CRC patients who underwent curative surgery, were included: (i) the discovery cohort (N = 124) with tumor tissues and postoperative plasmas for ctDNA determination; and (ii) the external validation cohort (N = 125) with available ctDNA results. In the discovery cohort, somatic variations in tumor tissues and plasmas collected postoperatively were determined via a 733-gene and 127-gene next-generation sequencing panel, respectively.
Results
In the discovery cohort, 17 of 108 (15.7%) patients had detectable ctDNA. ctDNA-positive patients had a significantly high recurrence rate (76.5% vs. 16.5%, P < 0.001) and short recurrence-free survival (RFS; P < 0.001) versus ctDNA-negative patients. In addition to ctDNA status, the univariate Cox model identified pathologic stage, lymphovascular invasion, nerve invasion, and preoperative carcinoembryonic antigen level associated with RFS. We combined the ctDNA and clinicopathological risk factors (CTCP) to construct a model for recurrence prediction. A significantly higher recurrence rate (64.7% vs. 8.1%, P < 0.001) and worse RFS (P < 0.001) were seen in the high-risk patients classified by the CTCP model versus those in the low-risk patients. Receiver operating characteristic analysis demonstrated that the CTCP model outperformed ctDNA alone at recurrence prediction, which increased the sensitivity of 2-year RFS from 49.6% by ctDNA alone to 87.5%. Harrell's concordance index, calibration curve, and decision curve analysis also suggested that the CTCP model had good discrimination, consistency, and clinical utility. These results were reproduced in the validation cohort.
Conclusion
Combining postoperative ctDNA and clinical risk may better predict recurrence than ctDNA alone for developing a personalized postoperative management strategy for CRC.
Analysing the probability of detection of shallow spherical defects by means of Active Thermography
Authors: D’Accardi Ester , Palumbo Davide , Errico Vito , Fusco Andrea , Angelastro Andrea , Galietti Umberto 
Published: October 2022

Abstract
The capability of Active Thermography (AT) techniques in detecting shallow defects has been proved by many works in the last years, both on metals and composites. However, there are few works in which these techniques have been used adopting simulated defects more representative of the real ones. The aim of this work is to investigate the capability of Active Thermography of detecting shallow spherical defects in metal specimens produced with laser powder bed fusion (L-PBF) process and characterized by a thermal behaviour very far from the flat bottom hole and so near to the real one. In particular, the quantitative characterization of defects has been carried out to obtain the Probability of Detection (PoD) curves. In fact, it is very common in non-destructive controls to define the limits of defect detectability by referring to PoD curves based on the analysis of flat bottom holes with a more generous estimation and therefore not true to real defect conditions. For this purpose, a series of specimens, made by means of Laser-Powder Bed Fusion technology (L-PBF) in AISI 316L, were inspected using Pulsed Thermography (PT), adopting two flash lamps and a cooled infrared (IR) sensor. To improve the quality of the raw thermal data, different post-processing algorithms were adopted. The results provide indications about the advantages and limitations of Active Thermography (AT) for the non-destructive offline controls of the structural integrity of metallic components.
Safety of sodium-glucose cotransporter 2 inhibitors (SGLT2i) during the month of Ramadan in patients with Type 2 Diabetes Mellitus in Pakistani population -an observational study from a tertiary care center in Karachi
Authors: Sheikh Aisha , Das Bhagwan , Sattar Saadia , Islam Najmul 
Published: October 2022

Abstract
Background and aims

Primary aim was to assess the safety of SGLT2-i in a real-life scenario during Ramadan by finding the frequency and severity of hypoglycemic/hyperglycemic events, dehydration, and DKA. Secondary aim was to assess changes in glycated hemoglobin (HbA1c), weight and creatinine levels.

Methods

This prospective, observational, controlled cohort study was conducted at Aga Khan University Hospital, Karachi, Pakistan from March 15 to June 30, 2021. Participants were over 21 years of age, on stable doses of SGLT2-I, started at least two months before Ramadan. Endpoint assessments were done one month before and within six weeks after Ramadan.

Results

Of 102 participants enrolled, 82 completed the study. Most (52%) were males, with mean age 52.2 ± 9.5 years and average duration of T2DM 11.2 ± 6.5 years. 63% were on Empagliflozin (mean dose; 14.8 ± 7.2 mg/day) whereas 37% were on Dapagliflozin (mean dose; 8.2 ± 2.7 mg/day). Six (7.3%) documented symptoms of hypoglycemia, however, no episode of severe hypoglycemia, hyperglycemia, dehydration, DKA, hospitalization or discontinuation of SGLT2i was reported. HbA1c changes were (7.7% ± 1.2% from 7.9% ± 2.3%, p 0.34), weight (78.4 ± 12.9 kgs from 78.9 ± 13.3, p 0.23) and eGFR (87.8 ± 27.9 from 94.3 ± 37.6, p < 0.001).

Conclusion

Results showed the safety of SGLT2i agents during Ramadan in Pakistani population recommending it as a treatment option in adults with T2DM, without any additional adverse events.
Hydrological variability and flood risk in a forest watershed undergoing accelerated urbanization: the case of Mefou (South Cameroon)
Authors: Ebode Valentin Brice 
Published: October 2022

Abstract
Climate change and variability and anthropogenic forcings such as land use change are the main forcings of river discharge variability and hydrological risks. However, these issues are very less addressed in central Africa. This article aims to investigate the impact of precipitation variability and land use change on the hydrological dynamics of the Mefou river over the recent period (1963–2018) and draw up a basin flooding risk areas map. To achieve these goals, hydrometeorological data of this basin were analyzed using the Pettitt and Mann Kendall tests. Likewise, flooding risk areas was produced from the Frequency Ratio (FR) model. Average and extreme flows of the Mefou river have increased since 1985-86, unlike the rainfall, which generally decreased for all seasons from the 1970s, apart from summer, where the reverse was observed. Changes in land use (an increase in impervious areas (+ 530%) and a decrease in forest (-52.9%) and water bodies(-80.4%)) seem to be the leading cause of the increase in flows observed. Floods are often recurrent in basins with such hydrological behaviour. To enable policymakers to reduce the vulnerability of populations to this disaster, the proposed flood map shows that 2.67%, 7.22%, 22.5%, 35.25% and 32.36% of the catchment area are respectively delineated into very low, low, medium, high and very high flood vulnerability classes. These results could be useful for the management of water resources and associated hydrological risks in the basin investigated.
Association of neutrophil-to-lymphocyte ratio and platelet-to-lymphocyte ratio with diabetic kidney disease in patients with type 2 diabetes
Authors: Li Lan , Shen Qing , Rao Sijie 
Published: October 2022

Abstract
Purpose
The neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) have been extensively studied in a variety of diseases. However, research on their relationship with diabetic kidney disease (DKD) is limited. The aim of our study was to investigate the association of these two indicators with renal function in type 2 diabetes patients and assess whether they can serve as predictors of DKD.
Methods
A total of 655 patients with type 2 diabetes were retrospectively selected. Subjects were divided into three groups according to the urinary albumin-to-creatinine ratio (UACR). The differences in the NLR and PLR among the groups and their correlation with renal function were analyzed. Logistic regression analysis was used to analyze independent risk factors for DKD, and receiver operating characteristic (ROC) curves were used to assess the predictive values of the NLR and PLR for the disease.
Results
The NLR and PLR were significantly different among the three groups, and they increased with increasing levels of albuminuria. Pearson’s correlation analysis showed that the NLR and PLR were positively correlated with the UACR but negatively correlated with the estimated glomerular filtration rate (eGFR) (p < 0.001). Logistic regression analysis showed that these two indicators were independent risk factors for DKD (p < 0.001). The results of ROC curve analysis suggested that the NLR (AUC = 0.794; 95% CI, 0.760–0.827; p < 0.001) and PLR (AUC = 0.665; 95% CI, 0.623–0.706, p < 0.001) had important diagnostic value for DKD.
Conclusion
The NLR and PLR were closely associated with renal function among type 2 diabetes patients, and high NLR and PLR may serve as predictors of DKD.