bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of hot spring spa-bathing habits on physical and mental health in Japan
Authors: Takeda Midori , Nakamura Hiroki , Otsu Hajime , Mimori Koshi , Managi Shunsuke 
Published: September 2022

Abstract
Hot springs have long been used for medical purposes throughout the world. However, their health effects are still largely unknown. Therefore, we aimed to examine the physical and mental effects of hot spring spa-bathing habits throughout Japan. We conducted a new online questionnaire survey throughout Japan that included questions on spa-bathing behavior, disease prevalence, subjective satisfaction, and lifestyle habits. To understand regional differences, we used previously reported questionnaire data as a comparison. Regarding mental effects, the results showed a significant positive correlation between spa-bathing habits and subjective satisfaction. Moreover, 2% of life satisfaction was explained by spa-bathing habits in the regression model. Regarding physical effects, we found a significant reduction in the odds ratio of hypertension among spa bathers in two populations: elderly people nationwide and elderly people in Beppu. In addition, among elderly individuals in Beppu, the odds ratios of depression and hyperlipidemia were significantly lower among spa bathers, suggesting that there may be differences in the effects of hot springs between regions. This study revealed that hot spring spa-bathing habits positively impacted both physical and mental health. Our findings suggest that spa bathing could improve health status, especially among elderly individuals, by preventing hypertension and improving mental health.
First insights into the genetic diversity of Spodoptera spp. (Lepidoptera: Noctuidae) affecting vegetable crops in Jamaica
Authors: Neath Damion Orlando , Robinson Dwight Edward , Tennant Paula Fredericka 
Published: September 2022

Abstract
Spodoptera spp. (Lepidoptera: Noctuidae) are economically important insect pests of cereal and vegetable crops in Tropical and Subtropical regions of the Americas, but little is known of their distribution and diversity in countries of the Caribbean, in particular, Jamaica. In this study, larvae collected from hosts in the three major vegetable producing regions of Jamaica were characterized based on morphological characters and mitochondrial cytochrome c oxidase I (COI) sequence data. Three species were identified; S. exigua, S. latisfascia and S. frugiperda. All three species were prevalent on bunching onions (Allium fistulosum); while bell pepper (Capsicum annum) and corn (Zea mays) were also hosts for S. exigua and S. frugiperda, respectively. Species clades at 99% confidence were obtained in phylogenetic analyses with Jamaican COI sequences and those from different geographical locations. Separate clustering occurred with native S. latisfascia and invasive S. exigua populations of the Americas. S. frugiperda corn and rice strain groupings that were closely related to native accessions of the Americas and invasives in Africa and Asia, including populations with recorded insecticide resistance, were distinguished. Based on this sampling, the Jamaican populations are consistent with those previously reported in the Americas and elsewhere. Low intraspecific genetic distances of ≤ 0.6% were obtained with the three species and apart from two exceptions, interspecific distances ranged from zero to 1.4%. The results provide new information about the population dynamics of Spodoptera species affecting vegetable crops in Jamaica and establish a baseline for further studies.
The anti-adipogenic effects of postbiotics derived from plant-based lactic acid bacteria
Authors: Yoon Da Hye , Kim Joo Yun , Keon Heo , Lee Hyeon Gyu , Seo Kunho , Shim Jae-Jung , Lee Jung-lyoul , Kim Hyunsook 
Published: September 2022

Abstract
The anti-obesity effects of postbiotics, non-viable heat killed lactic acid bacteria (Lactobacillus plantarum (HKLP) and Lactobacillus curvatus (HKLC)) were studied in 3T3-L1 cells along with their bioactive components, S-layer proteins (SLPs, LPSLP, and LCSLP). Relative lipid accumulation in 3T3-L1 adipocytes was significantly inhibited after HKLP, HKLC, HKLPC (a mixture of HKLP and HKLC), LPSLP, and LCSLP. Expression of adipogenic gene was significantly downregulated by the HKLPs and SLPs. Interestingly, SLPs induced apoptosis of 3T3-L1 preadipocytes and early adipocytes, which were determined by flow cytometry with Annexin V-FITC/propidium iodide staining. This was closely associated with a significant decrease in the expression of the anti-apoptosis gene, Bcl-2, and an increase in the pro-apoptosis gene, caspase-3. These results suggest that plant-based postbiotics, HKLABs and SLPs can be used as naturally occurring products in the prevention of obesity.
Relationship between Atmosphere of Psychiatric Wards and Motivation, Attitude and Perception of Patients Hospitalized in Shiraz and Bushehr University Hospitals - 2019
Authors: MA Abolhoseini , Bahreini M , Sh Pouladi , Bagherzadeh R 
Published: September 2022

Abstract
Research Background: Treatment of psychiatric patients is a challenging care issue that requires special attention due to the special nature and conditions of these patients. Detecting the factors affecting the acceptance of treatment by these patients can help control the disease and promote their health. The environment where people live can affect their behaviors. The present study aimed to determine the relationship between ward atmosphere with motivation, perception, and attitude of patients towards continuing the treatment process in psychiatric wards of certain university hospitals in Shiraz and Bushehr in 2019.
Methods: The present cross-sectional study included 299 patients admitted to the psychiatric wards of Bushehr and Shiraz hospitals. A convenience sampling method was performed. Data collection tools included demographic characteristics form, a short-form psychiatric ward questionnaire, and patient attitude, motivation, and treatment perception questionnaires. Data were analyzed using SPSS 19 and Pearson or Spearman correlation tests, independent t-test, one-way analysis of variance, and linear regression. The significance level was considered less than 0.05 in all cases.
Results: The mean score of ward atmosphere was lower than average (18.37±2.99) from the patients' point of view. Among the subscales of ward atmosphere from the patients' point of view, the "support" subscale was a predictor of attitude towards continuing the treatment process (P=0.042, β=-0.131). The negative attitude toward treatment was lower at higher levels of support. Two subscales, namely "support" (P=0.048, β=0.120) and "programme clarity" (P<0.001, β = 0.221) were predictors of patients' perception of the treatment continuation; in other words, the patients' perception of the treatment process was higher at higher levels of these two subscales. None of the ward atmosphere subscales were related to the patient's motivation to continue the treatment process from the patient's point of view. (P>0.05)
Conclusion: Given the relationship between at least some sub-scales of ward atmosphere and patients' attitudes and perceptions about continuing the treatment process, designing ward atmosphere optimization programs is recommended to improve patients' attitudes and perceptions to continue the treatment process. It is recommended to study other factors affecting treatment in psychiatric patients.
The clinical differences between patients with pheochromocytoma with hypertension and those with normotension
Authors: Liu Zhenghuan , Zhou Liang , Zhu Yuchun 
Published: September 2022

Abstract
Objective
To explore the clinical differences between patients with pheochromocytoma with hypertension and those with normotension. In addition, we aimed to determine the risk factors associated with the development of hypertension.
Methods
All patients in West China Hospital undergoing surgical removal of pheochromocytoma (PHEO) were retrospectively analyzed to determine those with hypertension and normotension from 5 January 2009 to 19 July 2018. Preoperatively, demographic and clinical data were recorded and analyzed.
Results
Finally, there were 164 (31.3%) patients with normotension and 360 (68.7%) patients with hypertension. Older patients and those with longer courses of PHEO were more likely to develop hypertension. Patients with hypertension were less likely to have extra-adrenal PHEO. In addition, patients with hypertension had higher blood glucose levels and were more likely to develop diabetes. Similarly, the white blood cell (WBC) and blood platelet (PLT) counts of patients with hypertension were both higher than those of patients with normotension. Last but not least, the blood norepinephrine of patients with hypertension, which was positively associated with tumor size, was higher.
Conclusions
A total of 31.3% of PHEO patients might be with normotension. Older patients and those with longer courses of PHEO were more likely to present hypertension preoperatively. Patients with hypertension had higher blood norepinephrine and blood glucose levels. They were also more likely to develop diabetes.
Successful Treatment of Pediatric Systemic Mastocytosis With Omalizumab: Case Report and Literature Review
Authors: Bossi Grazia , Brazzelli Valeria , De Amici Mara , Raviola Chiara , Naso Matteo , Regalbuto Corrado , Boselli Federica , Fortina Valeria , Marseglia Gian Luigi 
Published: September 2022

Abstract
Background. Pediatric Mastocytosis is a rare and heterogeneous disease, characterized by accumulation of mast cells in the skin (Cutaneous Mastocytosis) and/or, less frequently, in other organs, mainly liver, spleen, bone marrow, lymph nodes and gastrointestinal tract (Systemic Mastocytosis). Patients affected by Systemic Mastocytosis complaint symptoms caused by the massive release of mast cell mediators: itching, flushing, abdominal pain, generalized weakness, fatigue and neuropsychiatric disorders. Moreover, children with Systemic Mastocytosis are at greater risk of anaphylactic/anaphylactoid reactions, often poorly controlled by the conventional therapy with antihistamines, mast cells stabilizers and steroids. As a result, children affected by Systemic Mastocytosis have a poor quality of life and suffer the consequence of prolonged steroidal treatment.
Case presentation. A child with Systemic Mastocytosis and severe symptoms, refractory to symptomatic and steroidal therapy, has been successfully treated with Omalizumab, an anti-IgE monoclonal antibody usually employed in allergic patients with severe asthma and orticaria. The onset of clinical benefit of omalizumab therapy was extraordinarily rapid but proved to be strictly dependent on drug administration. The child has become completely and steadily asymptomatic. No other anaphylactic episodes have been reported. Steroid treatment could be definitively withdrawn and all the other medications were reduced. Omalizumab was well tolerated and no side effects have been observed.
Conclusions. In our experience Omalizumab is an effective treatment for children affected by Systemic Mastocytosis not responding to conventional medical treatments. The main strengths of this therapy are its rapid and extraordinary efficacy to control the severe mast cells mediator-related symptoms, the good safety profile and its steroid-sparing effect. However, more extensive and controlled studies in pediatric patients affected by Systemic Mastocytosis are needed to substantiate these promising findings.
The role of sclerostin and irisin on bone and muscle of orchiectomized rats
Authors: Zhou Bing-na , Zhang Qian , Lin Xiao-yun , Hu Jing , Zhao Di-chen , Jiang Yan , Xing Xiao-ping , Li Mei 
Published: September 2022

Abstract
Background: The reduction in androgen level gives rise to a decrease in bone mineral density and muscle strength,but the exact mechanisms are unclear. We investigated the roles of novel cytokines of sclerostin and irisin on bone and muscle of orchiectomized (ORX) rats.
Methods: Twenty 3-month-old male rats were randomized to receive sham or orchiectomy operation. Rats were euthanized after 8 weeks of surgery, and serum levels of sclerostin and irisin were measured by enzyme-linked immunosorbent assay at baseline and execution. Grip strength was measured by a grip strength tester at baseline and before execution. Bone mineral density (BMD) and bone microarchitecture were measured by microcomputed tomography. The samples of bone and muscle were harvested at execution. Bone biomechanics were measured by three-point bending tests and vertebral body indentation tests. Bone and muscle histological features were analyzed by hematoxylin and eosin stain, Von Kossa’s stain and tartrate resistant acid phosphatase stain. Simple linear regression analyses were used to analyze the relationships between serum levels of sclerostin, irisin and grip strength and BMD of ORX rats.
Results: Serum sclerostin level increased from 279±44 pg/mL to 586±57 pg/mL since baseline to 8 weeks after ORX (P=0.002), which was significantly higher than that in sham rats (406±20 pg/mL at execution) (P=0.012). The serum irisin level decreased from 4.12±0.20 ng/mL to 3.55±0.29 ng/mL since baseline to 8 weeks of ORX (P=0.048), which was significantly lower than sham rats (4.84±0.37 pg/mL at execution) (P=0.013). Trabecular BMD, parameters of bone microarchitecture, bone strength, grip strength and the myofibers size of soleus muscles were significantly lower in ORX rats than in sham group. Grip strength was positively correlated with femoral trabecular BMD (r=0.713, P<0.001) and bone volume/total volume (r=0.712, P<0.001) in all rats. The serum sclerostin level was negatively correlated to femoral trabecular BMD (r=-0.508, P=0.022) and grip strength (r=-0.492, P=0.028). Serum irisin level was positively correlated with femoral trabecular BMD (r=0.597, P=0.005), but no obvious correlation was found between irisin level and muscle strength in all rats.
Conclusions: Reduced BMD, impaired bone microarchitecture, weak strength of bone and muscle, and thin myofibers were induced by androgen deficiency of ORX rats. Serum sclerostin and irisin levels were significantly changed after ORX, which might be closely correlated with the occurrence of osteoporosis and sarcopenia in ORX rats.
A palynomorph ecological model for the Late Paleozoic successions of Indian coal deposits with special emphasis on Godavari Valley Coalfield: Conception and configuration
Authors: Aggarwal Neha 
Published: September 2022

Abstract
Based on the distribution of the vegetation and integration of the megafloral, palynological and micropalaeobotanical pieces of information, a palaeocommunity model is designed that may allow the detailed interpretations of the quantitative palynomorphs distribution patterns in the Permian (Asselian-Changhsingian) succession of the Indian coal deposits in terms of the variable palaeoenvironments with fluctuating water levels. The conceptual model is based on the identification of the Palynomorphs Ecogroups (PEGs) that reflect broad co-existing plant communities, adverse climatic, floodplain-swamp, lake/pond, stress-tolerant, swamp-floodplain, swamp-marsh, unknown affinity, upland and wide habitat PEGs. In successive palynological assemblages, shifts in the predominance and the sun-dominance of PEGs are thought to be the indicator of the water level fluctuations. Palaeoclimatic shifts may be predicted through significant fluctuations within the quantitative compositions of the individual PEGs.
Changes in Paraspinal Muscles after PLIF versus Percutaneous Endoscopic Transforaminal Lumbar Interbody Fusion (PE-TLIF) in Patients with Lumbar Spinal Stenosis: A One-Year Prospective Study
Authors: Pang Daming , Yang Jincai , Yin Peng , Hai Yong , Fan Zhexuan , Gao Haifeng 
Published: September 2022

Abstract
Background
Compared to PLIF, the advantages of PE-TLIF are its greater preservation of the posterior components of the lumbar spine and its reduction of damage to the paraspinal muscles. However, the full extent of postoperative paraspinal muscle changes after damage by PE-TLIF has remained largely unknown. This is the first study to compare the changes in paraspinal muscles between PLIF and PE-TLIF directly using CT and clinical effects.
Methods
This study included 52 patients with lumbar spinal stenosis who were treated at Beijing Chaoyang Hospital between January, 2020 and January, 2021. Among them, 22 patients received PLIF, and 30 received PE-TLIF. Outcome metrics including the ODI, VAS-LBP, and VAS-LP at pre-surgery and 1-week, 6-months, and 12-months post-surgery were used to evaluate the clinical effects of both procedures. Additionally, radiographic metrics including the MF FCSA, FI rate, and muscle density were used to evaluate changes in patients’ MF muscles.
Results
The PE-TLIF group has better performance on the VAS-back pain scale at the 1-day follow-up (PE-TLIF: 3.25 vs. PLIF: 4.32, P = 0.003) and 1-week follow-up (PE-TLIF: 2.53 vs. PLIF: 3.61, P༜0.001). At 6 months after surgery, there was no statistical difference in MF FCAS, FI rate, or CT density between the PLIF and PE-TLIF groups. There was also no statistically significant atrophy of the MF FCSA in both groups at the last (12-month) follow-up (PE-TLIF: 506.5 (488.0,535.0) mm2; PLIF: 512.0 (485.3,564.5) mm2, P༞0.05). At the 12-month follow-up, the FI rate in the MF muscles in the PLIF group was higher than that in the PE-TLIF group (PE-TLIF: 3.0 (2.8,3.0); PLIF:3.0 (3.0,4.0), P༜0.05), and the same result was also observed in CT density (PE-TLIF: 34.2 (31.8,36.9) Hu; PLIF: 30.5 (28.5,32.1) Hu, P < 0.05).
Conclusion
PE-TLIF can achieve satisfactory clinical results. After 12 months post-surgery, we observed no excess MF atrophy in the PE-TLIF group compared to the PLIF group, and the FI rate and muscle density of the MF in the PE-TLIF group were better than those in the PLIF group.
An interval aerobic training intervention on Heart rate variability and the selected biochemical factors in boys with type 1 diabetes
Authors: Saki Hossein , Nazem Farzad , Fariba Farnaz 
Published: September 2022

Abstract
Objective: The purpose of this research is to investigate the effect of interval aerobic (INTA) exercise training on heart rate variability (HRV), blood glucose, and plasma biomarkers levels in type 1 diabetic (T1D) adolescents.
Research design and methods: boys (12–18 years) were divided into three groups: health control (HC, n = 12), diabetic control (DC, n = 12) and diabetic training (DT, n = 12). Anthropometric, HRV (24 hours), biochemical parameters, blood glucose, and aerobic capacity (VO2peak) were evaluated at the baseline and end of 12-wk of the exercise training program.
Results: HRV, glycemic index, VO2peak, NEP, HDL-C indicated significant differences between the T1D groups with HC group at baseline condition (p < 0.001). Although, BMI, LDL-C, TC, and TG parameters were homogeneous in all groups. Meanwhile, HRV parameters, VO2peak and HDL-C, and NEP were significantly increased after exercise intervention. HbA1c, and blood glucose, levels were notably reduced after an INTA intervention (p > 0.001).
Conclusion: The present study revealed the necessity of early screening for CVD risk factors in T1D adolescents. Also, it was revealed INTA training compared to other training patterns, and cardiovascular health improves via enhancement of autonomic modulation, VO2peak, Fat profile, and catecholamine levels.
Optimization of ATAC-seq in wheat seedling roots using INTACT-isolated nuclei
Authors: Debernardi Juan Manuel , Burguener German , Bubb Kerry , Liu Qiujie , Queitsch Christine , Dubcovsky Jorge 
Published: September 2022

Abstract
Background:
The genetic information contained in the genome of an organism is organized in genes and regulatory elements that control gene expression. The genomes of multiple plants species have already been sequenced and the gene repertory have been annotated, however, cis-regulatory elements remain less characterized, limiting our understanding of genome functionality. These elements act as open platforms for recruiting both positive- and negative-acting transcription factors, and as such, chromatin accessibility is an important signature for their identification.
Results:
In this work we developed a transgenic INTACT [isolation of nuclei tagged in specific cell types] system in tetraploid wheat for nuclei purifications. Then, we combined the INTACT system together with the assay for transposase-accessible chromatin with sequencing [ATAC-seq] to identify open chromatin regions in wheat root tip samples. Our ATAC-seq results showed a large enrichment of open chromatin regions in intergenic and promoter regions, which is expected for regulatory elements and that is similar to ATAC-seq results obtained in other plant species. In addition, root ATAC-seq peaks showed a significant overlap with a previously published ATAC-seq data from wheat leaf protoplast, indicating a high reproducibility between the two experiments and a large overlap between open chromatin regions in root and leaf tissues. Importantly, we observed overlap between ATAC-seq peaks and cis-regulatory elements that have been functionally validated in wheat, and a good correlation between normalized accessibility and gene expression levels.
Conclusions:
We have developed and validated an INTACT system in tetraploid wheat that allows rapid and high-quality nuclei purification from root tips. Those nuclei were successfully used to performed ATAC-seq experiments that revealed open chromatin regions in the wheat genome that will be useful to identify cis-regulatory elements. The INTACT system presented here will facilitate the development of ATAC-seq datasets in other tissues and growth stages to generate a more complete landscape of the accessible DNA regions in the wheat genome.
AgMYB5, an MYB transcription factor from celery, enhanced β- carotene synthesis and promoted drought tolerance in transgenic Arabidopsis
Authors: Sun Miao , Xu Qin-Yi , Zhu Zhi-Peng , Liu Pei-Zhuo , Yu Jian-Xiang , Guo Yao-Xian , Tang Shu , Yu Zhi-Fang , Xiong Ai-Sheng 
Published: September 2022

Abstract
Background
Water shortage caused by global warming seriously affects the yield and quality of vegetable crop. β-carotene, the lipid-soluble natural product with important pharmacological value, is abundant in celery. Transcription factor MYB family extensively disperses in plants and plays regulatory roles in carotenoid metabolism and water scarcity response.
Results
Here, AgMYB5 gene encoding 196 amino acids was amplified from celery cv. ‘Jinnanshiqin’. In celery, the expression of AgMYB5 exhibited transactivation activity, tissue specificity, and drought-condition responsiveness. Further analysis proved that ectopic expression of the AgMYB5 increased β-carotene content and promoted drought resistance in transgenic Arabidopsis thaliana. Moreover, AgMYB5 expression promoted β-carotene biosynthesis by triggering the expression of AtCRTISO and AtLCYB, which in turn increased antioxidant enzyme activities, and led to the decreased contents of H2O2 and MDA, and the inhibition of O2− generation. Meanwhile, β-carotene accumulation promoted endogenous ABA biosynthesis of the transgenic Arabidopsis plants overexpression AgMYB5 gene, which resulted in ABA-induced stomatal closing and delayed water loss rate. In addition, ectopic expression of AgMYB5 increased expression levels of AtERD1, AtP5CS1, AtRD22, and AtRD29.
Conclusions
The findings indicated that AgMYB5 up-regulated β-carotene biosynthesis and drought resistance of Arabidopsis.