bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel treatment and derivatization for quantification of residual aromatic diisocyanates in polyamide resins
Authors: Pastore Genny , Gabrielli Serena , Leone Ezio , Cortese Manuela , Gentili Dario , Biondi Giovanna , Marcantoni Enrico 
Published: July 2022

AbstractIn the scientific context, the environmental and healthy impact of polymers is more related to the residual monomer content rather than their macromolecular structure, due to the monomer capability to interact with membrane cells. For this a novel method to stabilize and quantify residual monomeric isocyanates in high thermal resistance polyamide resins (PAs) has been developed. This new analytical method resulted in an improvement concerning the quantification of residual aromatic diisocyanates in viscous polymeric matrices by using a simple and cheap technique like HPLC-VWD. Diisocyanate monomers were derivatized with dibutylamine, resulting in stable urea derivatives that were simultaneously analysed and quantified. The method was applied to solvent-based polyamide resins, used as primary electrical insulation, for avoiding additional step of solvent removing before the analysis. The quantification of residual monomers answers to the provisions imposed by European Regulation N. 1907/2006 (REACH) for polymer registration, and the necessity of an early evaluation of the occupational risk associated with the use of diisocyanates, due to their toxicity and high reactivity towards moisture.
Using HPV-meta for human papillomavirus RNA quality detection
Authors: Ure Agustin , Mukhedkar Dhananjay , Arroyo Mühr Laila Sara 
Published: July 2022

AbstractIn the era of cervical cancer elimination, accurate and validated pipelines to detect human papillomavirus are essential to elucidate and understand HPV association with human cancers. We aimed to provide an open-source pipeline, “HPV-meta”, to detect HPV transcripts in RNA sequencing data, including several steps to warn operators for possible viral contamination. The “HPV-meta” pipeline automatically performs several steps, starting with quality trimming, human genome filtering, HPV detection (blastx), cut-off settlement (10 reads and 690 bp coverage to make an HPV call) and finishing with fasta sequence generation for HPV positive samples. Fasta sequences can then be aligned to assess sequence diversity among HPV positive samples. All RNA sequencing files (n = 10,908) present in the cancer genome atlas (TCGA) were analyzed. “HPV-meta” identified 25 different HPV types being present in 488/10,904 specimens. Validation of results showed 99.98% agreement (10,902/10,904). Multiple alignment from fasta files warned about high sequence identity between several HPV 18 and 38 positive samples, whose contamination had previously been reported. The “HPV-meta” pipeline is a robust and validated pipeline that detects HPV in RNA sequencing data. Obtaining the fasta files enables contamination investigation, a non very rare occurrence in next generation sequencing.
Inoculation effect of Pseudomonas sp. TF716 on N2O emissions during rhizoremediation of diesel-contaminated soil
Authors: Kim Ji-Yoon , Cho Kyung-Suk 
Published: July 2022

AbstractThe demand for rhizoremediation technology that can minimize greenhouse gas emissions while effectively removing pollutants in order to mitigate climate change has increased. The inoculation effect of N2O-reducing Pseudomonas sp. TF716 on N2O emissions and on remediation performance during the rhizoremediation of diesel-contaminated soil planted with tall fescue (Festuca arundinacea) or maize (Zea mays) was investigated. Pseudomonas sp. TF716 was isolated from the rhizosphere soil of tall fescue. The maximum N2O reduction rate of TF716 was 18.9 mmol N2O g dry cells−1 h−1, which is superior to the rates for previously reported Pseudomonas spp. When Pseudomonas sp. TF716 was added to diesel-contaminated soil planted with tall fescue, the soil N2O-reduction potential was 2.88 times higher than that of soil with no inoculation during the initial period (0–19 d), and 1.08–1.13 times higher thereafter. However, there was no enhancement in the N2O-reduction potential for the soil planted with maize following inoculation with strain TF716. In addition, TF716 inoculation did not significantly affect diesel degradation during rhizoremediation, suggesting that the activity of those microorganisms involved in diesel degradation was unaffected by TF716 treatment. Analysis of the dynamics of the bacterial genera associated with N2O reduction showed that Pseudomonas had the highest relative abundance during the rhizoremediation of diesel-contaminated soil planted with tall fescue and treated with strain TF716. Overall, these results suggest that N2O emissions during the rhizoremediation of diesel-contaminated soil using tall fescue can be reduced with the addition of Pseudomonas sp. TF716.
A multiplex PCR assay for the identification of fruit flies (Diptera: Tephritidae) of economic importance in South Africa
Authors: Andrews Kelsey J. , Bester Rachelle , Manrakhan Aruna , Maree Hans J. 
Published: July 2022

AbstractThe fruit fly (Diptera: Tephritidae) species,Ceratitis capitata,Ceratitis cosyra,Ceratitis rosa,Ceratitis quilicii, andBactrocera dorsalisare of economic importance in South Africa. These agricultural pests cause extensive damage to a range of commercially produced fruit, primarily for export. These pests are of phytosanitary significance, and their presence in fruit-producing regions in South Africa has led to restrictions in export trade of fresh produce. Accurate identification of these flies, particularly at immature stages intercepted in fruit consignments originating from South Africa, is essential but remains an ongoing challenge. A rapid and accurate identification assay to differentiate these five species is needed for inspection and pest surveillance. High throughput sequencing data were generated for each of the five fruit fly species, and five sets of species-specific primers were designed for use in a multiplex PCR. Each primer set amplifies an amplicon of a different size for each species allowing for accurate identification. PCR sensitivity tests demonstrate that the limit of detection for this assay is 10 ng and 4 ng of DNA when extracted from larvae and adult specimens, respectively. The assay developed can be applied in fruit inspection and survey activities within the country and at ports of entry.
Research on shifting process control of automatic transmission
Authors: Zou Wujun , Wang Ye , Zhong Chaojie , Song Zhenchuan , Li Shenlong 
Published: July 2022

AbstractThe shift quality of an automatic transmission directly affects the human-perceived comfort and the durability of the automatic transmission. In general, the inconsistency caused by manufacturing errors, life-cycle changes, or other changes in hydraulic characteristics are the main reason affecting the shift quality, which should be compensated by adaptive control in the shifting process. In this paper, we first provide an in-depth analysis of the relationship between proportional solenoid current, clutch pressure, speed and torque in the shifting process control. Then we propose two efficient adaptive control strategies for the torque phase and inertia phase, respectively. Both algorithms are tested and verified on a riot utility vehicle. The experimental results indicate that the adaptive control strategies proposed in this paper can effectively compensate the engine flare and the clutch tie-up of the torque phase, and keep the inertia phase within a proper time range.
Reliability of the active drag assessment using an isotonic resisted sprint protocol in human swimming
Authors: Gonjo Tomohiro , Olstad Bjørn Harald 
Published: July 2022

AbstractThe purpose of the presents study was to investigate the reliability of the active drag (Da) assessment using the velocity perturbation method (VPM) with different external resisted forces. Eight male and eight female swimmers performed 25 m sprints with five isotonic loads (1–2–3–4–5 kg for females; 1–3–5–7–9 kg for males), which were repeated twice on different days. The mean velocity and semi-tethered force were computed for each condition, and the free-swimming maximum velocity was estimated with load-velocity profiling. From the obtained variables, Da at the maximum free-swimming condition was calculated using VPM. Absolute and typical errors and the intra-class correlation (ICC) were calculated to assess test–retest reliability. 95% confidence interval (95% CI) lower bound of ICC was larger than 0.75 in 3, 4 (females only) and 5 kg trials in both sexes (corresponding to 37–60 N additional resistance; all p < 0.001), which also showed small absolute and relative typical errors (≤ 2.7 N and ≤ 4.4%). In both sexes, 1 kg load trial (16–17 N additional resistance) showed the lowest reliability (95% CI of ICC; − 0.25–0.83 in males and 0.07–0.94 in females). These results suggested that a tethered force of 37–60 N should be used to assess Da using VPM.
Surgical and functional outcomes of robot-assisted versus laparoscopic partial nephrectomy with cortical renorrhaphy omission
Authors: Kubota Masashi , Yamasaki Toshinari , Murata Shiori , Abe Yohei , Tohi Yoichiro , Mine Yuta , Hagimoto Hiroki , Kokubun Hidetoshi , Suzuki Issei , Tsutsumi Naofumi , Inoue Koji , Kawakita Mutsushi 
Published: July 2022

AbstractTo evaluate the surgical and functional outcomes between robot-assisted (CRO-RAPN) vs. laparoscopic (CRO-LPN) methods of cortical-renorrhaphy-omitting partial nephrectomy. Between July 2012 and June 2020, patients with localized clinical T1-2 renal masses who underwent CRO-RAPN or CRO-LPN were reviewed. The outcomes of the two groups were compared using propensity-score matching. Trifecta was defined as negative surgical margin, warm ischemic time < 25 min, and absence of complications of Clavien-Dindo grade III or more until three months postoperatively. The preservation rate of the estimated glomerular filtration rate (eGFR) was evaluated at six months postoperatively. Among 291 patients (CRO-RAPN, n = 210; CRO-LPN, n = 81) included in the study, 150 matched pairs of patients were analyzed. Compared to the CRO-LPN group, the CRO-RAPN group was associated with shorter warm ischemic time (13 min vs. 20 min, P < 0.001), shorter total operation time (162 min vs. 212 min, P < 0.001), less estimated blood loss (40 mL vs. 119 mL, P = 0.002), lower incidence of overall complications (3% vs. 16%, P = 0.001), higher preservation rate of eGFR at six months postoperatively (93% vs. 89%, P = 0.003), and higher trifecta achievement rate (84% vs. 64%, P = 0.004). CRO-RAPN contributed to shorter warm ischemic time, less blood loss, fewer complications, and higher preservation of renal function, all of which allowed this technique to achieve a higher rate of trifecta compared to CRO-LPN.
Human antibodies to SARS-CoV-2 with a recurring YYDRxG motif retain binding and neutralization to variants of concern including Omicron
Authors: Liu Hejun , Kaku Chengzi I. , Song Ge , Yuan Meng , Andrabi Raiees , Burton Dennis R. , Walker Laura M. , Wilson Ian A. 
Published: July 2022

AbstractStudying the antibody response to SARS-CoV-2 informs on how the human immune system can respond to antigenic variants as well as other SARS-related viruses. Here, we structurally identified a YYDRxG motif encoded by IGHD3-22 in CDR H3 that facilitates antibody targeting to a functionally conserved epitope on the SARS-CoV-2 receptor binding domain. A computational search for a YYDRxG pattern in publicly available sequences uncovered 100 such antibodies, many of which can neutralize SARS-CoV-2 variants and SARS-CoV. Thus, the YYDRxG motif represents a common convergent solution for the human humoral immune system to target sarbecoviruses including the Omicron variant. These findings suggest an epitope-targeting strategy to identify potent and broadly neutralizing antibodies for design of pan-sarbecovirus vaccines and antibody therapeutics.
Asymmetric subgenomic chromatin architecture impacts on gene expression in resynthesized and natural allopolyploid Brassica napus
Authors: Li Zeyu , Li Mengdi , Wang Jianbo 
Published: July 2022

AbstractAlthough asymmetric subgenomic epigenetic modification and gene expression have been revealed in the successful establishment of allopolyploids, the changes in chromatin accessibility and their relationship with epigenetic modifications and gene expression are poorly understood. Here, we synthetically analyzed chromatin accessibility, four epigenetic modifications and gene expression in natural allopolyploid Brassica napus, resynthesized allopolyploid B. napus, and diploid progenitors (B. rapa and B. oleracea). “Chromatin accessibility shock” occurred in both allopolyploidization and natural evolutionary processes, and genic accessible chromatin regions (ACRs) increased after allopolyploidization. ACRs associated with H3K27me3 modifications were more accessible than those with H3K27ac or H3K4me3. Although overall chromatin accessibility may be defined by H3K27me3, the enrichment of H3K4me3 and H3K27ac and depletion of DNA methylation around transcriptional start sites up-regulated gene expression. Moreover, we found that subgenome Cn exhibited higher chromatin accessibility than An, which depended on the higher chromatin accessibility of Cn-unique genes but not homologous genes.
Biogeographic implication of temperature-induced plant cell wall lignification
Authors: Crivellaro Alan , Piermattei Alma , Dolezal Jiri , Dupree Paul , Büntgen Ulf 
Published: July 2022

AbstractMore than 200 years after von Humboldt’s pioneering work on the treeline, our understanding of the cold distribution limit of upright plant growth is still incomplete. Here, we use wood anatomical techniques to estimate the degree of stem cell wall lignification in 1770 plant species from six continents. Contrary to the frequent belief that small plants are less lignified, we show that cell wall lignification in ‘woody’ herbs varies considerably. Although trees and shrubs always exhibit lignified cell walls in their upright stems, small plants above the treeline may contain less lignin. Our findings suggest that extremely cold growing season temperatures can reduce the ability of plants to lignify their secondary cell walls. Corroborating experimental and observational evidence, this study proposes to revisit existing theories about the thermal distribution limit of upright plant growth and to consider biochemical and biomechanical factors for explaining the global treeline position.
Whirling interlayer fields as a source of stable topological order in moiré CrI3
Authors: Ghader Doried , Jabakhanji Bilal , Stroppa Alessandro 
Published: July 2022

AbstractThe moiré engineering of two-dimensional magnets opens unprecedented opportunities to design novel magnetic states with promises for spintronic device applications. The possibility of stabilizing skyrmions in these materials without chiral spin-orbit couplings or dipolar interactions is yet to be explored. Here, we investigate the formation and control of ground state topological spin textures (TSTs) in moiré $${Cr}{I}_{3}$$
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using stochastic Landau–Lifshitz–Gilbert simulations. We unveil the emergence of interlayer vortex and antivortex Heisenberg exchange fields, stabilizing spontaneous and field-assisted ground state TSTs with various topologies. The developed study accounts for the full bilayer spin dynamics, thermal fluctuations, and intrinsic spin-orbit couplings. By examining the effect of the Kitaev interaction and the next nearest-neighbor Dzyaloshinskii–Moriya interaction, we propose the latter as the unique spin-orbit coupling mechanism compatible with experiments on monolayer and twisted $${Cr}{I}_{3}$$
C
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. Our findings contribute to the current knowledge about moiré skyrmionics and uncover the nature of spin-orbit coupling in $${Cr}{I}_{3}$$
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.
Enhanced CACIS configuration for direction of arrival estimation
Authors: Zhang Yule , Hu Guoping , zhang Fei , Zhou Hao 
Published: July 2022

AbstractIn this letter, an enhanced coprime array with compressed inter‐element spacing is designed for direction of arrival estimation. First, we analyse and conclude that the 0th sensor of coprime array with compressed inter‐element spacing is redundant for the consecutive lags, thus it is rearranged to the negative part to obtain enhanced coprime array with compressed inter‐element spacing. Then, on the basis of discussing the effect of the shift sensor on the difference co‐array, the number of unique lags and the range of consecutive lags are derived. Theoretical properties show that the closed‐form expressions of coprime array with compressed inter‐element spacing and enhanced coprime array with compressed inter‐element spacing are similar, while more difference lags can be generated from the latter. Moreover, larger inter‐element spacing can slightly alleviate the mutual coupling. Numerical examples verify the advantages of enhanced coprime array with compressed inter‐element spacing in direction of arrival estimation over coprime array with compressed inter‐element spacing configuration and other coprime arrays.
Multiwavelength observations of Swift J0243.6+6124 from 2017 to 2022
Authors: Liu Wei , Yan Jingzhi , Reig Pablo , Wang Xiaofeng , Xiao Guangcheng , Lin Han , Zhang Xinhan , Sai Hanna , Chen Zhihao , Yan Shengyu , Liu Qingzhong 
Published: July 2022

Context.Swift J0243.6+6124 is a high-mass X-ray binary that went into a giant X-ray outburst in 2017. During this event, the X-ray luminosity reached the highest value ever measured in a galactic Be/X-ray binary.
Aims. Our aim is to study the long-term variability of Swift J0243.6+6124 after the 2017 major X-ray outburst.
Methods. We have obtained optical spectroscopy and photometry data during four years after the event. The long-term photometric light curve and the equivalent widths of the Hα and He I λ6678 lines were used to monitor the state of the Be star’s circumstellar disk. The Hα line profiles show evidence for V/R variability that was accounted for by fitting the Hα spectral line profile with two Gaussian functions. We divided our data into three phases according to the intensity of the X-ray, optical, and infrared emission.
Results. Phase I covers the rise and decay of the giant X-ray outburst that took place in October–November 2017. We interpret phase II as the dissipation of the Be star’s equatorial disk and phase III as its recovery. The timescale of a complete formation and dissipation process is about 1250 days. The epoch when the dissipation process stopped and the reformation period began is estimated to be around MJD 58530. We find a delay of ∼100–200 days between the minimum of the optical or infrared intensity and the strength of the Hα line after the X-ray outburst, which may indicate that the dissipation of the disk begins from the inner parts. The motion of the density perturbation inside the disk is prograde, with a V/R quasi-period of about four years. The source shows a positive correlation in the (B − V) color index versus V-band magnitude diagram, which implies that the system is seen at a small or moderate inclination angle.
Conclusions. Despite the super-Eddington X-ray luminosity during the outburst, the subsequent pattern of long-term optical and IR variability of Swift J0243.6+6124 is typical of Be/X-ray binaries.
Fetal Cardiovascular MRI – A Systemic Review of the Literature: Challenges, New Technical Developments, and Perspectives
Authors: Knapp Janine , Tavares de Sousa Manuela , Schönnagel Björn P. 
Published: July 2022

Background Fetal magnetic resonance imaging (MRI) has become a valuable adjunct to ultrasound in the prenatal diagnosis of congenital pathologies of the central nervous system, thorax, and abdomen. Fetal cardiovascular magnetic resonance (CMR) was limited, mainly by the lack of cardiac gating, and has only recently evolved due to technical developments.Method A literature search was performed on PubMed, focusing on technical advancements to perform fetal CMR. In total, 20 publications on cardiac gating techniques in the human fetus were analyzed.Results Fetal MRI is a safe imaging method with no developmental impairments found to be associated with in utero exposure to MRI. Fetal CMR is challenging due to general drawbacks (e. g., fetal motion) and specific limitations such as the difficulty to generate a cardiac gating signal to achieve high spatiotemporal resolution. Promising technical advancements include new methods for fetal cardiac gating, based on novel post-processing approaches and an external hardware device, as well as motion compensation and acceleration techniques.Conclusion Newly developed direct and indirect gating approaches were successfully applied to achieve high-quality morphologic and functional imaging as well as quantitative assessment of fetal hemodynamics in research settings. In cases when prenatal echocardiography is limited, e. g., by an unfavorable fetal position in utero, or when its results are inconclusive, fetal CMR could potentially serve as a valuable adjunct in the prenatal assessment of congenital cardiovascular malformations. However, sufficient data on the diagnostic performance and clinical benefit of new fetal CMR techniques is still lacking.Key Points: Citation Format
Summary of Bifurcation Stenting Strategies and Outcomes in Women
Authors: Alasnag Mirvat , Dakhil Zainab , Sukmawati Indah , Rao Sarita , Hussain Shazia T. 
Published: July 2022

AbstractSeveral studies have examined the efficacy of drug-eluting stents in acute and chronic coronary syndromes and demonstrated benefit in both men and women. However, the effect of gender on percutaneous coronary intervention (PCI) outcomes in more complex disease including bifurcation disease is less well-known. In particular, a two-stent or provisional strategy, for both left Main and non-Left Main bifurcation lesions, has been variable across the different studies. In addition, there is a notable geographic preference whereby a stepwise provisional strategy with culotte as a bailout technique is preferred in Europe while in Asia there is a predilection to DK Crush for a 2-stent strategy. Many of these trials, however, are underpowered to conduct subgroup analysis for outcomes in women. To date, the evidence suggests women have better outcomes with coronary artery bypass grafting in complex disease. The higher events in women undergoing PCI are largely driven by bleeding. Studies dedicated to evaluating outcomes after complex revascularization in women are desperately needed with long-term follow-up. This review examines the current landmark trials as they pertain to outcomes in women.
Artificial Intelligence Methods for Fault Diagnosis in Centrifugal Pumps
Authors: Al Tobi Maamar Ali Saud , Bevan Geraint , Okedu Kenneth 
Published: July 2022

This important book offers a foundation for use of artificial intelligence (AI) in fault diagnosis and classification for centrifugal pumps. It outlines methods for operators to identify and classify faults that are not easily detectable using traditional techniques like time domain and frequency domain methods. It brings together different AI approaches that are integrated with Wavelet Transform (WT) and Genetic Algorithm (GA) into a single reference—making advanced diagnostic methods accessible to engineers who may not have a computer science background.
Covering a wide range of AI applications for mechanical systems, the book:Advances readers from description of the problem to application of AI methods for diagnosticsOutlines ways for operators to identify and classify faults that are not easily detectable using traditional techniquesOffers new data from a novel experimental rig not previously available
Artificial Intelligence Methods for Fault Diagnosis in Centrifugal Pumps is an ideal reference for academics, engineers, and industry professionals working in plant and operations maintenance. Undergraduate and graduate students of artificial intelligence systems will find this an invaluable reference.