bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of molecular patterns and prognostic models of epithelial–mesenchymal transition- and immune-combined index in the gastric cancer
Authors: Zhang Xiuyuan , Li Yiming , Hu Pengbo , Xu Liang , Qiu Hong 
Published: August 2022

Background: Epithelial–mesenchymal transition (EMT) and the immune microenvironment play important roles in the progression of gastric cancer (GC), but the joint role of both in GC is not clear.Methods: We identified EMT- and immune-related genes (EIRGs), and the molecular subtypes of EIRGs were identified by unsupervised cluster analysis. Then, we constructed an accurate EIRG_score model by using differential genes of molecular subtypes. The correlation of EIRG_score with prognosis, immune infiltration, gene mutation, chemotherapeutic drug sensitivity, and immunotherapy response was comprehensively analyzed. In addition, we investigated the biological function of EIRG_score via in vitro experiments.Results: A total of 808 GC patients were classified into two molecular subtypes, which were enriched in EMT and immune-related biological pathways and significantly correlated with prognosis and immune infiltration. The constructed EIRG_score had an important role in predicting prognosis and immunotherapeutic response. The higher EIRG_score was associated with worse prognosis, higher abundance of immunosuppressive cell infiltration, lower immune checkpoint genes expression, lower tumor mutation burden, microsatellite instability-high, lower chemotherapeutic drug sensitivity, and poorer immunotherapeutic response.Conclusion: EIRG_score may be used as a biomarker to assess prognosis and guide precise treatment.
Implementation of exercise countermeasures during spaceflight and microgravity analogue studies: Developing countermeasure protocols for bedrest in older adults (BROA)
Authors: Hedge Eric T. , Patterson Courtney A. , Mastrandrea Carmelo J. , Sonjak Vita , Hajj-Boutros Guy , Faust Andréa , Morais José A. , Hughson Richard L. 
Published: August 2022

Significant progress has been made in the development of countermeasures to attenuate the negative consequences of prolonged exposure to microgravity on astronauts’ bodies. Deconditioning of several organ systems during flight includes losses to cardiorespiratory fitness, muscle mass, bone density and strength. Similar deconditioning also occurs during prolonged bedrest; any protracted time immobile or inactive, especially for unwell older adults (e.g., confined to hospital beds), can lead to similar detrimental health consequences. Due to limitations in physiological research in space, the six-degree head-down tilt bedrest protocol was developed as ground-based analogue to spaceflight. A variety of exercise countermeasures have been tested as interventions to limit detrimental changes and physiological deconditioning of the musculoskeletal and cardiovascular systems. The Canadian Institutes of Health Research and the Canadian Space Agency recently provided funding for research focused on Understanding the Health Impact of Inactivity to study the efficacy of exercise countermeasures in a 14-day randomized clinical trial of six-degree head-down tilt bedrest study in older adults aged 55–65 years old (BROA). Here we will describe the development of a multi-modality countermeasure protocol for the BROA campaign that includes upper- and lower-body resistance exercise and head-down tilt cycle ergometry (high-intensity interval and continuous aerobic exercise training). We provide reasoning for the choice of these modalities following review of the latest available information on exercise as a countermeasure for inactivity and spaceflight-related deconditioning. In summary, this paper sets out to review up-to-date exercise countermeasure research from spaceflight and head-down bedrest studies, whilst providing support for the proposed research countermeasure protocols developed for the bedrest study in older adults.
Fostering Academic Engagement in Post-graduate Students: Assessing the Role of Positive Emotions, Positive Psychology, and Stress
Authors: Saleem Muhammad Shoaib , Isha Ahmad Shahrul Nizam , Awan Maheen Iqbal , Yusop Yuzana Binti , Naji Gehad Mohammed Ahmed 
Published: August 2022

AimThe current study attempted to assess the effect of positive emotion on post-graduate students’ psychological capital (PsyCap) as well as on their academic engagement behavior. Also, the direct relationship between PsyCap and academic engagement behavior was assessed alongside the presence of Stress as a moderating variable between PsyCap and academic engagement behavior amongst post-graduate students in Malaysia.Materials and MethodsA self-administered questionnaire was used for data collection from 373 post-graduate Ph.D. students registered in various universities throughout Malaysia with a non-probabilistic sampling technique. Research respondents belonged to management, humanities, engineering, computer science, and health sciences domains, and they responded through a questionnaire copy. Statistically, structural equation modeling (SEM) was applied to evaluate confirmatory factor analysis (CFA), reliability analysis, validity analysis, measurement model, structural model, and path analysis. Furthermore, the (5000) bootstrapping approach was utilized to test the final model.ResultsFor the hypothesized model, our results confirmed that positive emotions had a positive and significant effect on students’ psychological capita as well as on their academic engagement behavior. Further, PsyCap also had a positive and significant effect on academic engagement behavior. Our results also reported that stress as a moderating variable has a negative and deteriorating effect on the relationship between PsyCap and the academic engagement of students.ConclusionThe study’s findings support the theoretical assumption that positive emotions help individuals generate cognitive resources, which in turn help them manage their engagement behavioral requirements. However, the stress caused by their study needs may deplete their psychological resources, consequently influencing their academic engagement behavior. Interventions like personal coaching/counseling, appropriate follow-up, and flexible goal settings with other measures may help post-graduate students in achieving their daunting tasks.
Combined balance and plyometric training enhances knee function, but not proprioception of elite male badminton players: A pilot randomized controlled study
Authors: Zhou Limingfei , Gong Wangcheng , Wang Shixian , Guo Zhenxiang , Liu Meng , Chuang Samuel , Bao Dapeng , Zhou Junhong 
Published: August 2022

ObjectivesTo investigate the effect of combined balance and plyometric training on knee function and proprioception of elite badminton athletes.MethodsSixteen elite male badminton players (age: 20.5 ± 1.1 years, height: 177.8 ± 5.1 cm, weight: 68.1 ± 7.2 kg, and training experience: 11.4 ± 1.4 years) volunteered to participate and were randomly assigned to a combined balance and plyometric training (CT) (n = 8) and plyometric (PT) group (n = 8). The CT group performed balance combined with plyometric training three times a week over 6 weeks (40 min of plyometrics and 20 min of balance training); while the PT group undertook only plyometric training for the same period (3–4 sets × 8–12 reps for each exercise). Both groups had the same technical training of badminton.ResultsThe knee function and proprioception were assessed at baseline and after the intervention by measuring the performance of single-legged hop tests (LSIO, LSIT, LSIC, LSIS), standing postural sway (COPAP, COPML), and LSI of dominant leg and non-dominant leg. The results showed that as compared to PT, CT induced significantly greater improvements in LSIT and LSIS (p &lt; 0.001) and significant greater percent increase in NAP (p = 0.011). The changes in LSIO, LSIC, DAP, NAP, LSIAP, DML, NML, and LSIML induced by CT did not differ from that induced by PT (p &gt; 0.213).ConclusionIn elite badminton players, intervention using CT holds great promise to augment the benefits for knee function compared to the intervention using PT only, and at the same time, with at least comparable benefits for proprioception. Future studies are needed to examine and confirm the results of this study.
Quality of life changes and their predictors in young adult narcolepsy patients after treatment: A real-world cohort study
Authors: Chin Wei-Chih , Wang Chih-Huan , Huang Yu-Shu , Hsu Jen-Fu , Chu Kuo-Chung , Tang I , Paiva Teresa 
Published: August 2022

BackgroundWe conducted a five-year prospective follow-up study to track the real-world quality of life of patients with narcolepsy after medication and analyzed predictors.MethodsThe study ultimately included 157 participants who completed 5-year follow-up, 111 had type 1 narcolepsy (NT1) and 46 had type 2 narcolepsy (NT2). Polysomnography, multiple sleep latency test, actigraphy and HLA-typing were conducted. The Short Form 36 Health Survey Questionnaire (SF-36), the Stanford Center for Narcolepsy Sleep Inventory, the Epworth Sleepiness Scale (ESS), the visual analog for hypersomnolence (VAS), and Conners' Continuous Performance Test were used. Descriptive statistics, repeated measures, and hierarchical linear models were applied for analysis.ResultsMost demographic and clinical data did not significantly differ between groups, but the NT1 group had significantly more overweight, more severe narcoleptic symptoms, more positive HLA typing, shorter mean sleep latency, and more sleep onset rapid eye movement periods. No significant change to the physical domains of SF-36 was found in the total group, but we observed significant changes in emotional role functioning and social function. The NT1 group showed significant improvements in physical role functioning, emotional role functioning, and social function. The NT2 group demonstrated significant improvements in emotional role functioning. At the baseline, the NT2 group had significantly better scores, but there was no significant group difference after treatment, except for physical and social function. ESS and VAS were significantly improved during follow-up. At the baseline, the NT1 group had significantly higher ESS and VAS scores, and continuously significantly higher ESS scores during follow-up. Narcolepsy types, HLA typing, age of onset, symptom severity, attention and vigilance were significantly correlated with SF-36.ConclusionSymptom control greatly associates with the quality of life in narcoleptic patients, and medication can play the most important role. Management targeting narcoleptic symptoms, attention impairment, and drug adherence should be provided.
The association of COVID-19 vaccine availability with mental health among adults in the United States
Authors: Shen Chan , Rashiwala Lucy , Wiener R. Constance , Findley Patricia A. , Wang Hao , Sambamoorthi Usha 
Published: August 2022

ObjectiveTo assess whether COVID-19 vaccine approval and availability was associated with reduction in the prevalence of depression and anxiety among adults in the United States.MethodsWe adopted cross sectional and quasi-experimental design with mental health measurements before vaccine availability (June 2020, N = 68,009) and after vaccine availability (March 2021, N = 63,932) using data from Census Pulse Survey. Depression and anxiety were derived from PHQ-2 and GAD-2 questionnaires. We compared rates of depression and anxiety between June 2020 and March 2021. Unadjusted and adjusted analysis with replicate weights were conducted.ResultsDepression prevalence was 25.0% in June 2020 and 24.6% in March 2021; anxiety prevalence was 31.7% in June 2020 and 30.0% in March 2021 in the sample. In adjusted analysis, there were no significant differences in likelihood of depression and anxiety between June 2020 and March 2021.ConclusionDepression and anxiety were not significantly different between June 2020 and March 2021, which suggests that the pandemic effect continues to persist even with widespread availability of vaccines.
The pattern of lymph node metastasis in peripheral pulmonary nodules patients and risk prediction models
Authors: Ke Lei , Ma Honghai , Zhang Qingyi , Wang Yiqing , Xia Pinghui , Yu Li , Lv Wang , Hu Jian 
Published: August 2022

BackgroundFor peripheral pulmonary nodules, the regularity of lymph node (LN) metastasis has not been studied. This study aimed to evaluate the metastasis pattern of intrapulmonary and relevant mediastinal lymph nodes in early-stage lung cancer, and further selected patients who were of low risk of LN metastasis as potential population to receive sub-lobectomy.MethodsThis study prospectively included consecutive patients with peripheral clinical T1N0M0 disease who underwent complete resection with LN dissection or sampling from August 2014 to July 2015. The patients were followed up to 15, May 2021. Univariable or multivariable Logistic analysis was used to identify the risk factors. Models predicting LN metastasis risk were conducted. The area under the curve for the receiver operating characteristic curves was used to evaluate the diagnostic value. Disease-free survival and overall survival were compared between groups.ResultsFinally, 201 patients were included in this study. For patients with negative tumor-bearing (TB) 13 and 14 station LNs, the positive rate of other lymph node stations was extremely low. Maximum CT value, pleural indentation and CEA level were risk factors for N1 station LNs metastasis. Besides, the factors above and lobulation sign were risk factors for skip metastasis beyond TB 13 and 14 station LNs. We constructed two scoring tables to predict N1 station metastasis and skip metastasis beyond TB 13 and 14 station. The AUC were 0·837 and 0·823, respectively. Based on the first table, 40·9% of patients suffered N1 station LNs metastasis and 27·3% had N2 disease in “high risk group” while the proportion was only 5·7% and 4·5% in “low risk group”. For patients with negative TB13 and TB14 station LNs, based on the latter table, 11·1% of patients had N1 stations LNs metastasis and 16·7% had pN2 disease in “high risk group” while only 2·3% patients in “low risk group” suffered this kind of metastasis.ConclusionFor peripheral pulmonary nodules patients, stations 13 and 14 LNs may be the sentinel nodes. For patients with low risk of N1 metastasis and skip metastasis, sub-lobar resection might be sufficient for those who were of negative TB 13 and 14 station LNs.
The role of postoperative radiotherapy in patients with uterine sarcomas: A PSM-IPTW analysis based on SEER database
Authors: Hao Zhimin , Yang Sufen 
Published: August 2022

ObjectiveThe optimal adjuvant therapy for uterine sarcomas remains poorly determined due to its rarity and histological diversity. The purpose of the study is to explore and characterize the association between utilization of radiotherapy and survival outcome in patients with surgically resected uterine sarcomas.MethodsWe collected data regarding uterine sarcomas which were confirmed after total hysterectomy between 2010 and 2018 period from the latest version of the Surveillance, Epidemiology, and End Results (SEER) database. Initially, 1-, 3- and 5-year survival rate were calculated to predict potential risk factors and possible role of adjuvant chemotherapy and radiotherapy. Propensity score matching (PSM) and inverse probability treatment weighting (IPTW) technique were employed to balance confounding factors in the utilization of additional therapy. Multivariate and exploratory subgroup analyses were respectively conducted to evaluate the impact of adjuvant therapy on overall survival (OS) and cause-specific survival (CSS).ResultsA total of 2897 patients were enrolled in the analysis. Survival benefit at 1-, 3-and 5-year after initial treatment was observed in the group of radiotherapy given, however, poorer prognosis in the group of chemotherapy administration. Accordingly, chemotherapy was enrolled as a confounding factor when stratifying and matching patients by receipt of radiotherapy. Prior to and after PSM-IPTW adjustment, radiotherapy both demonstrated beneficial effect on OS and CSS based on multivariate analysis. Further subgroup analysis indicated radiotherapy improved OS and CSS among a subset of patients in stage II-IV, particularly with uterine leiomyosarcoma, tumor grade IV, bigger tumor size than 100 mm and even with chemotherapy administration.ConclusionsAdjuvant radiotherapy in uterine sarcomas after hysterectomy might be underutilized, and proper use of adjuvant radiotherapy combined with chemotherapy after surgery in advanced-stage and high-risk patients might improve survival.
COVID-19 Information-Seeking, Health Literacy, and Worry and Anxiety During the Early Stage of the Pandemic in Switzerland: A Cross-Sectional Study
Authors: Ilic Anica , Roser Katharina , Sommer Grit , Baenziger Julia , Mitter Vera Ruth , Mader Luzius , Dyntar Daniela , Michel Gisela 
Published: August 2022

Objectives: To describe COVID-19 information-seeking behavior (CISB) during the first stage of the pandemic in Switzerland and identify its determinants.Methods: We conducted an online cross-sectional survey (4 May to 6 July 2020). Participants self-reported their CISB (information sources and frequency), personal COVID-19 situation (e.g., perception about having had COVID-19), sociodemographic information, and completed validated measures of health literacy, and worry and anxiety. Data were analyzed using descriptive statistics and logistic regressions.Results: We included 1,505 participants (24.7% male; mean age = 43.0 years, SD = 13.9). Most participants reported searching for information daily (n = 1,023, 68.0%) and referring to multiple information sources (mean 3.7, SD = 1.5). Commonly used sources were official websites (n = 1,129, 75.0%) and newspapers (n = 997, 66.2%). Participants with higher health literacy were more likely to seek information daily and use online resources, but less likely to use personal networks than those with lower health literacy. We did not find any association between CISB and worry and anxiety.Conclusion: More opportunities for personal dialogue and education about reliable online information resources should be encouraged to optimize the CISB of groups with lower health literacy.
An Analysis of Mobility Influence in Optoelectronics Parameters in an InGaN/GaN Blue LED
Authors: Zarate-Galvez Sarai , Garcia-Barrientos Abel , Ambrosio-Lazaro Roberto , Garcia-Ramirez Mario , Stevens-Navarro Enrique , Plaza-Castillo Jairo , Hoyo-Montaño Jose , Perez-Cortes Obed 
Published: August 2022

Simulations on mobility influence in optoelectronics parameters from an InGaN/GaN blue LED using the Nextnano++ software arepresented in this paper. These simulations were performed by changing the hole and electron mobility value for the material compounds according to experimental, theoretical, and doping-concentration data already reported in the literature. The power law mobility is used for the current calculation in the quantum drift-diffusion model. The results indicate the lower hole and electron leakage currents correspond to the lowest mobility values for the InGaN alloy, the greatest amount of recombination occurs in the extreme wells within the active layer of the LED and the stable emission is at 3.6 V with peak wavelength λ^LED=456.7 nm and full width at half maximum FWHM~11.1 nm for the three mobilities. Although experimental and theoretical mobility values reach higher carrier density and recombination, the photon emission is broader and unstable. Additionally, the doping-concentration mobility results in lower wavelength shifts and narrows FWHM, making it more stable. The highest quantum efficiency achieved by doping-concentration mobility is only in the breakdown voltage (ηdop−max=60.43%), which is the IQE value comparable to similar LEDs and is more useful for these kinds of semiconductor devices.
Seasonal Xylem Sap Acidification Is Governed by Tree Phenology, Temperature and Elevation of Growing Site
Authors: Pramsohler Manuel , Lichtenberger Edith , Neuner Gilbert 
Published: August 2022

pH of xylem sap (pHx) was determined in three trees (Malus domestica (apple tree), Picea abies and Pinus cembra) in response to seasonal changes. Conifer trees from lowland (600 m) were compared to trees growing at the alpine timberline (1950 m a.s.l.). Xylem sap was extracted with a Scholander pressure bomb and pHx was measured with a pH microsensor. In all species, pHx changed markedly with season. In spring, pHx was acidic; during winter, the pHx was more alkaline. In apple trees, the pHx did not show a significant correlation with temperature but was rather affected by developmental stage. During flushing in spring, xylem sap acidification took place concomitant to the developmental stage “tight cluster”, when foliar development enables a significant transpiration and a consequent movement of water in the xylem. The xylem sap of the two studied conifers showed a significantly larger seasonal alkalinisation (+2.1) than found in apple trees (+1.2) and was significantly more pronounced at the timberline. Xylem sap acidification took place before bud break. pHx had a significant negative correlation with soil temperatures and corresponded to already reported pHx of angiosperms. Overall, pHx appears to be a sensitive stress marker and indicator of activity status in tree xylem.
LA EXPRESIÓN DEL PROMOTOR DEL GEN ahybadh4 EN PLANTAS TRANSGÉNICAS DE Arabidopsis thaliana ES ESPECÍFICA EN LA RAÍZ
Authors: Onofre Janette , Legaria-Solano Juan , Avonce Nelson , Iturriaga Gabriel 
Published: August 2022

La glicina betaína es uno de los solutos compatibles con el metabolismo más eficiente. En las plantas, la biosíntesis de la glicina betaína se lleva a cabo en dos pasos enzimáticos. Primero se oxida el sustrato colina a betaína aldehído por medio de la colina monooxigenasa (CMO); el segundo paso es catalizado por una betaína aldehído deshidrogenasa (BADH) que convierte la betaína aldehído a glicina betaína. Estudios previos mostraron que la transcripción del gen ahybadh17, que codifica para la BADH de Amaranthus hypochondriacus, es inducida por estrés hídrico y salinidad, en paralelo a un incremento en los niveles de glicina betaína. En el presente trabajo se analizó la expresión del promotor del gen ahybadh4 de A. hypochondriacus en plantas transgénicas de Arabidopsis thaliana. Para este propósito se construyó un gen quimérico con una secuencia de 1.5 kb correspondiente a la posible región promotora del gen ahybadh4 fusionada con el gen reportero uid A de la -glucuronidasa (GUS). Las semillas de las plantas transgénicas (T3) obtenidas, fueron crecidas durante 7, 14 y 21 días después de la germinación y fueron sometidas a tratamientos de estrés osmótico y ácido abscísico (ABA) por un periodo de 16 horas. Los análisis histoquímico y fluorimétrico de GUS mostraron una expresión constitutiva y específica en la raíz. Estos resultados permiten concluir que la secuencia 5’ de 1.5 kb del gen ahybadh4 no se regula por estrés ósmótico o ABA en A. thaliana.
Effect of the Fiber Orientation of Glass Fiber Reinforced Polymer Composites on Mechanical Properties
Authors: CHIHAI (PEȚU) Rodica , UNGUREANU Claudia , BRIA Vasile 
Published: August 2022

Fiber reinforced polymer (FRP) composites possess excellent specific strength, specific stiffness and controlled anisotropy for which these are extensively used in various engineering applications, like automobile industries, aerospace industries, marine industries, space industries, electronics industries and many more. Glass fibers (GF), carbon fibers (CF) and aramid fibers (AF) are common reinforcements for polymer matrix composites (PMCs). High mechanical properties and wear resistance behaviour of glass fiber reinforced composites are the premises for the current experimental research on the effect of fiber orientation on the tensile strength of the polymeric composite materials. The glass fiber reinforced epoxy resin composite was prepared by wet lay-up method, followed by thermal treatment.