bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cholecystectomy reduces the risk of myocardial and cerebral infarction in patients with gallstone-related infection
Authors: Park Seon Mee , Kim Hyun Jung , Kang Tae Uk , Swan Heather , Ahn Hyeong Sik 
Published: June 2022

Abstract
Aim: We compared the risk of myocardial infarction (MI) or cerebral infarction (CI) in patients with or without-gallstone-related infection (GSI) and change in the risk following cholecystectomy.Methods: GSI (n=84,467) and non-GSI (n=406,800) patients with age- and sex-matched controls (n=4,912,670) were identified from Korean population based data. The adjusted hazard ratios (aHRs) of MI or CI were analyzed in both groups treated with or without cholecystectomy. Subgroup analysis was performed for both sex and different ages. Results: The risk of MI or CI was higher in the GSI group than in the non-GSI group (aHR for MI; 1.32 vs. 1.07, aHR for CI; 1.24 vs. 1.06, respectively). The risk reduction rate of MI following cholecystectomy was 11.4% in the GSI group, whereas it was 0% in the non-GSI group. The risk of CI after cholecystectomy was more reduced in the GSI group than in the non-GSI group (16.1% and 4.7%, respectively). The original risk of MI or CI in patients with gallstone and risk reduction rates following cholecystectomy were higher in females and younger patients than in males and older patients.Conclusion: Increased risk of MI or CI and greater risk reduction following cholecystectomy were seen in patients with GSI.
Clinical Course of Acute Exacerbation of Fibrosing Interstitial Lung Disease
Authors: Furuta Katsuyuki , Fujimoto Daichi , Matsunashi Atsushi , Morimoto Takeshi , Tanaka Masanori , Shibaki Ryota , Shimada Yuri , Nagata Kazuma , Tomii Keisuke , Yamamoto Nobuyuki 
Published: June 2022

Abstract
No large-scale studies have reported on the clinical course of acute exacerbation of fibrosing interstitial lung disease (AE-FILD). We aimed to elucidate the clinical course of AE-FILD. We retrospectively included patients admitted with AE-FILD diagnosis. The clinical courses of AE-FILD were classified as consistent improvement after response to treatment (responder group); no response to treatment (non-responder group); improvement after treatment followed by worsening respiratory status not attributable to AE-FILD (complication group); and improvement after treatment followed by worsening respiratory status attributable to AE-FILD relapse (second-attack group). Among 340 patients, 123 died within 90 days, 265 responded, and 75 were refractory to initial treatment. Among patients who responded to treatment, 191, 26, 46, and 2 belonged to the responder, complication, second-attack, and unknown groups, respectively. Multivariate analysis revealed that high lactate dehydrogenase, high C-reactive protein, and low PaO2/FiO2 were associated with 90-day mortality (odds ratio [OR] 2.27, p = 0.002; OR 2.01, p = 0.008; and OR 1.70, p = 0.042, respectively). Of patients who responded to treatment, a second attack was strongly associated with 90-day mortality (OR 18.54, p < 0.001). AE-FILD has a heterogeneous clinical course. Importantly, worsening of respiratory condition despite an initial response to treatment is common.
Genome-wide methylation profiling identify hypermethylated HOXL subclass genes as potential markers for esophageal squamous cell carcinoma detection
Authors: Yu Qiuning , Xia Namei , Zhao Yanteng , Jin Huifang , Chen Renyin , Ye Fanglei , Chen Liyinghui , Xie Ying , Wan Kangkang , Zhou Jun , Zhou Dihan , Lv Xianping 
Published: June 2022

Abstract
Background：Numerous studies have revealed aberrant DNA methylation in esophageal squamous cell carcinoma (ESCC). However, they often focused on the partial genome, which resulted in an inadequate understanding of the shaped methylation features and the lack of available methylation markers for this disease.Methods: The current study investigated the methylation profiles between ESCC and paired normal samples using whole-genome bisulfite sequencing (WGBS) data and obtained a group of differentially methylated CpGs (DMC), differentially methylated regions (DMR), and differentially methylated genes (DMG). The DMGs were then verified in independent datasets and Sanger sequencing in our custom samples. Finally, we attempted to evaluate the performance of these genes as methylation markers for the classification of ESCC and multiple cancer types.Results: We obtained 438,558 DMCs, 15,462 DMRs, and 1,568 DMGs. The four significantly enriched gene families of DMGs were CD molecules, NKL subclass, HOXL subclass, and Zinc finger C2H2-type. The HOXL subclass homeobox genes were observed extensively hypermethylated in ESCC and nine other cancer types. The HOXL-score estimated by HOXC10 and HOXD1 methylation showed good ability in discriminating ESCC from normal samples, while the methylation of GSX1 displayed potential utility for pan-cancer detection.Conclusions: We observed widespread hypomethylation events in ESCC, and the hypermethylated HOXL subclass homeobox genes presented promising applications for the early detection of multiple cancer types.
Tumor-derived Exosomal HOTAIRM1 Regulates SPON2 in CAFs to Promote Progression of Lung Adenocarcinoma
Authors: Chen Zhipeng , Bian Chengyu , Huang Jingjing , Li Xiang , Chen Liang , Xie Xueying , Xia Yang , Yin Rong , Wang Jun 
Published: June 2022

Abstract
Objective: SPON2 is one of the extracellular matrix proteins, which is closely related to the progression of a variety of tumors including non-small cell lung cancer (NSCLC), but its upstream regulation mechanism remains unclear. Our research aims to find the specific regulatory pathway of SPON2 by exploring the potential crosstalk between tumor cells and cancer-associated fibroblasts (CAFs) in tumor microenvironment (TME) of NSCLC. Methods: We analyzed T1 lung adenocarcinoma samples from TCGA and screened extracellular matrix proteins that indicate poor prognosis. Expression level of SPON2 was verified by qRT-PCR in clinical samples. The exosomes of NSCLC cell supernatant were extracted and identified by nanoparticle tracking analysis (NTA) and transmission electron microscope, western blots. The exosomes and CAFs were co-cultured, and cell migration and Matrigel invasion assay were used to evaluate the effect of CAFs on the migration and invasion of NSCLC cells. The interaction between LncRNA and miRNA was verified by Targetscan prediction, luciferase reporter assay, and RNA binding protein immunoprecipitation (RIP). Results: We found that the expression of SPON2 was up-regulated in clinical T1a stage NSCLC patients. The expression of lnc HOTAIRM1 (HOTAIRM1) in exosomes secreted by NSCLC tissues increased. After exosomal HOTAIRM1 entered CAFs, HOTAIRM1 can adsorb miR-328-5p to up-regulate the expression of SPON2 in CAFs. Up-regulation of SPON2 in CAFs could promote the metastasis and invasion of NSCLC cells. Conclusion Tumor-derived exosomal HOTAIRM1 can transfer into CAFs and competitively adsorb miR-328-5p, and regulate the SPON2 expression of CAFs cells, ultimately promote the progression of NSCLC. The discovery of this regulatory pathway can provide a new potential therapeutic target for the diagnosis and treatment of NSCLC.
RBM10, a new potential RBP function, recruits METTL3 to induce N6-methyladenosine-MALAT1-dependent modification on inhibiting the invasion and migration of NSCLC
Authors: Cao Yingshu , Di Xin , Cong Shan , Tian Chang , Wang Yan , Jin Xin , Zhao Min , Zhou Xijia , Li Ranwei , Wang Ke 
Published: June 2022

Abstract
Background: Previous studies have shown that RBM10 is a potential tumor suppressor protein that can inhibit proliferation and promote apoptosis of NSCLC. RBM10 can bind and modify RNA at the post transcriptional level.Method and results: Transwell and cell wound healing assay showed that RBM10 significantly inhibited the invasion and migration of NSCLC. CLIP-seq showed that among all RBM10 binding RNAs, lncRNA MALAT1 with high m6A methylation in lung cancer had the highest binding peak among all non-coding RNAs. RNA Immunoprecipitation has verified the direct combination of RBM10 and MALAT1. The rescue experiment confirmed that RBM10 affected the phosphorylation of PI3K/AKT/mTOR passway protein, the invasion and migration ability by regulating MALAT1. MeRIP-qPCR confirmed that RBM10 could inhibit MALAT1 m6A methylation levels by recruiting METTL3.Conclusion: These results suggest that RBM10, as an RNA-binding protein, may inhibit m6A methylation of MALAT1 by recruiting METTL3, and affect phosphorylation of the downstream PI3K/AKT/mTOR pathway by binding and regulating MALAT1, ultimately affecting the invasion and migration of NSCLC.
Prevalence of Undernutrition and Associated Factors among Patients with Cardiac Diseases in Public Hospitals, Addis Ababa, Ethiopia
Authors: Feleke Atsedemaryam , Chane Yawukal , Azale Telake 
Published: June 2022

Abstract
Background
Under-nutrition among patients with cardiac disease is a public health challenge globally, particularly in developing nations including our country. Cardiac cachexia is recently receiving growing attention as modern treatment options prevent early death from cardiac events and more patients live with chronic compensated heart failure. However, information on the magnitude in developing countries specifically in the study area is very scarce.
Methods
Institution based cross-sectional study was conducted among randomly selected 343 cardiac patients from four public hospitals. Data were collected by trained nurses with face-to-face interview using structured questionnaire after consents are secured. The data were entered in to Epi Data version 3.1 and cleaned and analyzed by SPSS version 25. Descriptive statistics, binary and multivariable logistic regression analyses were applied to identify factors associated with under-nutrition. P-value less than 0.05 with 95% confidence interval was considered statistically significant.
Results
The prevalence of under-nutrition among adult cardiac patients on follow up at Addis Ababa public hospitals was 144 (42.6%), 95% CI (37.4–47.9). The study participants who earn below 1050 ETB per household per month, AOR = 7.07, 95%CI: (3.22, 15.53), NYHA heart failure classification stage III and IV: AOR = 6.56, 95%CI (3.52, 12.24), those with additional illness: AOR = 1.80, 95%CI (1.05, 3.01), current khat chewers: AOR = 2.73, 95%CI (1.09, 6.81), those without nutritional care and support: AOR = 2.02, 95%CI (1.09, 3.72) were significantly associated with under-nutrition.
Conclusions
The prevalence of under-nutrition among adult patients with cardiac diseases was higher compared with many other studies in low and middle income countries. Poverty, comorbid diseases, substance use, and severe form of cardiac disease were significant predictors of under-nutrition. It is high time to carry out nutritional assessment of patients with cardiac diseases and counsel patients about strict adherence to medical recommendations including stopping substance use.
Association study of 45 SNPs in a non-help seeking cohort sample with at-risk mental state for psychosis
Authors: Marques Julia Hatagami , Talib Leda Leme , Hortêncio Lucas , Andrade Julio Cesar , Alves Tania Maria , Serpa Mauricio Henriques , Yamamoto Guilherme Lopes , de Bilt Martinus Theodorus van , Rössler Wulf , Gattaz Wagner Farid , Loch Alexandre Andrade 
Published: June 2022

Abstract
Background: At-risk mental state for psychosis (ARMS) samples are highly heterogeneous and non-epidemiological, as most of the studies recruit help-seeking patients from specialized clinics. Transition-to-psychosis rates differ among them, ranging from 7.4% to 54%, raising a question on the real power of clinical ARMS`s criteria alone to predict transition. Finding a biomarker for transition would improve the prediction rates when associated with clinical high-risk criteria.Methods: We genotyped 45 single nucleotide polymorphisms (SNPs) among a control sample and a non-help seeking ARMS sample and compared their frequencies. Then, we followed up this ARMS cohort of 90 individuals for 2.5 years and compared the genotype frequencies between non-converters and psychotic converters and non-psychotic converters.Results: We found a trend for a higher frequency of the TT genotype of rs6277, a dopamine receptor D2 gene (DRD2) SNP, among those who transitioned to psychosis (p=0.004) and among those who converted to any psychiatric diagnose (p=0.003), which did not remain significant after correction with Benjamini-Hochberg procedure (critical value=0.001). Converters and non-converters could not be differentiated from one another by any other characteristic. There were no significant differences between the genotype frequencies in the control group and the ARMS group in the initial cross section evaluation. Transition rates in our non-help-seeking ARMS sample were 3.33% for psychosis and 13.33% for non-psychotic disorders, a total of 16.66% for psychiatric disorders in general. Conclusions: Our findings suggest that rs6277 should be investigated in a larger ARMS sample to better assess its possible relation to transition to psychosis and other psychiatric disorders.
Solid-type poorly differentiated adenocarcinoma of the stomach: a characteristic morphology reveals a distinctive immunoregulatory tumor microenvironment
Authors: Kawatoko Shinichiro , Kohashi Kenichi , Torisu Takehiro , Sasaki Taisuke , Umekita Shinya , Oki Eiji , Nakamura Masafumi , Kitazono Takanari , Oda Yoshinao 
Published: June 2022

Abstract
Purpose
Solid-type poorly differentiated adenocarcinoma (solid-type-PDA) of the stomach is a unique histological subtype of “tubular adenocarcinoma”, but little is known about its clinicopathological features, molecular pathological characteristics and immunoregulatory tumor microenvironment.
Methods
We examined the immunohistochemical expressions of mismatch repair (MMR) proteins (MLH1, PMS2, MSH2, MSH6) in 57 cases of solid-type-PDA and classified them as either MMR-deficient or -proficient (dMMR, N = 23; pMMR, N = 34). We additionally identified 18 dMMR-well-differentiated adenocarcinoma (WDA) and 34 pMMR-WDA as control groups. We analyzed and compared solid-type-PDA with WDA by evaluating the immunoexpressions of key immune pathway proteins (programmed death ligand 1 (PD-L1) and indoleamine 2,3-dioxygenase 1 (IDO1)) and tumor-infiltrating lymphocytes (TILs) (CD8, Foxp3 and PD-1).
Results
IDO1 was significantly more frequent in dMMR-solid-type-PDA than in dMMR-WDA (P = 0.0046). Moreover, dMMR-solid-type-PDA tended to have higher mean CD8 + and Foxp3 + TILs compared with dMMR-WDA [P = 0.0006 (CD8+) and P = 0.1061 (Foxp3+)], and IDO1-positive tended to be associated with a large number of CD8+, Foxp3 + or PD-l + TILs in almost all tumor subtypes. PD-L1 was significantly observed in 44% (15/34) of pMMR-solid-type-PDA compared with 18% (6/34) of pMMR-WDA (P = 0.0344). Although they are molecularly and morphologically classified as the same chromosomal instability subtype, overall survival (OS) and disease-free-survival (DFS) in pMMR-solid-type-PDA were significantly worse than those in pMMR-WDA [P = 0.0216 (OS) and P = 0.0160 (DFS)].
Conclusion
Our study demonstrates that immunoexpressions of several immunoregulatory proteins and TILs are more prevalent in dMMR-solid-type-PDA, potentially a useful discovery for designing tumor treatments with immune checkpoint inhibitors or combination therapies with a PD-1/PD-L1-inhibitor and IDO1-inhibitor.
Numerical Analyses of Long Fibers Reinforced Polymeric Sheets Processed by Single Point Incremental Forming
Authors: Conte Romina , Giuseppe Serratore , Ambrogio Giuseppina , Francesco Gagliardi 
Published: June 2022

Abstract
Polymeric matrix composites (PMCs) have gained increasing relevance in different industrial applications and their employment results to be a necessity in the production of lightweight structures. The manufacturing solutions, which allow to properly shape PMCs panels, need molds for shaping the material reducing the process flexibility. In this context, the single point incremental forming (SPIF) could be a valuable process solution if properly customized to the PMCs properties. Herein, a possible process variant is introduced and its capability in forming long fibers reinforced thermoplastics was evaluated. To achieve this aim, a numerical model was implemented focusing the attention, first, on the material properties that have to be considered for a proper model construction. The performed numerical simulations showed the applicability of SPIF to shape PMC sheets. Furthermore, the executed simulations pointed out the influences of some variables on the quality of the formed parts showing possible arising of defects, such as wrinkling and rippled surfaces, at different process conditions and providing a first proof of concept of the proposed working solution.
Lay-Cardiopulmonary resuscitation after out-of-hospital cardiac arrest in aneurysmal subarachnoid hemorrhage patients - a retrospective multi-centric study
Authors: Pantel Tobias , Neulen Axel , Mader Marius , Kurz Elena , Fassl Verena , Westphal Manfred , Ringel Florian , Czorlich Patrick 
Published: June 2022

Abstract
Introduction
Aneurysmal subarachnoid hemorrhage (SAH) may be associated with sudden cardiac arrest (CA). Since there are many data on the positive impact of lay resuscitation on patient survival, but data are rare for SAH patients, our study aimed to investigate this aspect.
Methods
In this multi-center retrospective analysis, we reviewed the local SAH databases for OHCA and CPR from 01/2011 to 12/2020. Data on CPR were extracted from the ambulance and emergency room (ER) reports. Information on clinical management after hospital admission were gathered from the institutional health records. Collected data were correlated with patient survival as defined outcome parameter.
Results
We screened 1120 patients with SAH and identified 38 patients with SAH, OHCA and CPR (3.4%). Our cohort showed a high proportion of posterior circulation aneurysms (n = 17, 44.7%). Lay CPR was performed in 20 patients. Time to CPR was significantly shorter for lay CPR (p < 0.001). 17 patients (44.7%) were not initially assigned to CCT, resulting in a significantly prolonged time interval (154 ± 58 vs. 40 ± 23 min; p < 0.033). Overall survival rate of patients with OHCA and CPR was 29.7% (11 patients). However, pre-hospital lay CPR (p = 0.632) and shorter duration to the initial CCT after admission (p = 0.065) did not influence the outcome.
Conclusions
Sudden OHCA occurs regularly in patients with SAH. A timely initiated and high-quality CPR is crucial to facilitate patient’s initial survival. Although prolonged time to CCT does not affect survival, SAH should be considered as a cause of CA and CCT should be integrated into the diagnostic algorithm.
Complete chloroplast genomes of Sorbus sensu stricto (Rosaceae): comparative analyses and phylogenetic relationship
Authors: Tang Chenqian , Chen Xin , Deng Yunfei , Geng Liyang , Ma Jianhui , Wei Xueyan 
Published: June 2022

Abstract
Background: Sorbus sensu stricto (Sorbus s.s.) is a genus with important economical values for its beautiful leaves, flowers, especially the colorful fruits in tribe Maleae of the family Rosaceae, and comprises about 90 species mainly distributed in China. There are considerable disputes on the infrageneric classification and species delimitation for the morphological similarities of species. With the aim to shed light on the circumscription of taxa within the genus, phylogenetic analyses were performed with 29 Sorbus s.s. chloroplast (cp) genomes (16 newly sequenced) representing two subgenera and eight sections. Results: The 16 cp genomes newly sequenced range between 159,646 bp and 160,178 bp in length. All the samples examined and 22 taxa re-annotated in Sorbus sensu lato (Sorbus s.l.) contain 113 unique genes and 19 of these duplicated in the inverted repeat (IR). Gene deletions detected previously caused mainly by references selected. Six hypervariable regions including trnR-atpA, petN-psbM, rpl32-trnL, trnH-psbA, trnT-trnL and ndhC-trnV were screened, 44–53 SSRs and 58–130 dispersed repeats were identified as potential molecular markers. Phylogenetic analyses under ML/BI indicated that Sorbus s.l. is polyphyletic, Sorbus s.s. and the other five segregated genera, Aria, Chamaemespilus, Cormus, Micromeles and Torminalis are monophyletic. Two major clades and four sub-clades resolved with full-support within Sorbus s.s. are inconsistent with the existing infrageneric classification. Two subgenera, subg. Sorbus and subg. Albocarmesinae are supported to be monophyletic when S. tianschanica transferred to subg. Albocarmesinae from subg. Sorbus and S. hupehensis var. paucijuga transferred to subg. Sorbus from subg. Albocarmesinae, respectively. The classification at the sectional level is not supported by analysis of cp genome phylogeny.Conclusion: Phylogenomic analyses of the cp genomes are useful for inferring phylogenetic relationships in Sorbus s.s. Though genome structure is highly conserved in the genus, hypervariable regions and repeat sequences detected are the most promising potentially molecule makers for population genetic, species delimitation and phylogenetic studies.
Anatomical study of the maxillary sinus: which characteristics can influence its volume?
Authors: Lessa Anne Maria Guimaraes , de Oliveira Vitor Silva , Costa Roberta Basanez Aleluia , Meneses Alana Tavares Ribeiro , Crusoé-Rebello Iêda Margarida , Costa Fábio Wildson Gurgel , Neves Frederico Sampaio 
Published: June 2022

Abstract
Purpose: To investigate whether there is a relationship between the volume of the maxillary sinus and individual parameters such as sex, side, posterior tooth absence, sinus membrane thickening, bony septa, and vertical and sagittal skeletal patterns.Methods: The tomographic volume of the maxillary sinus from 211 individuals (422 sides) was evaluated using Horos DICOM Viewer software. Bone septa and sinus membrane thickening were classified as absent or present, while maxillary posterior dental absence was defined as the absence of one or more teeth in the posterior region of the maxilla (except for the third molars). The test-test was applied to analyze maxillary sinus volume according to sex, age, side, posterior tooth absence, sinus membrane thickening, and bony septa. For comparison of sagittal and vertical patterns, a one-way analysis of variance (ANOVA) with Tukey’s post-hoc test was applied.Results: No statistically significant differences were observed between the maxillary sinus volume according to gender (p=0.06), side (p=0.37), posterior tooth absence (p=0.92), sinus membrane thickening (p=0.47), bony septa (0.89) and vertical skeletal pattern (p=0.67). In relation to the sagittal skeletal pattern, a statistically significant difference was observed between Classes II and III (p=0.05).Conclusion: Maxillary sinus volume was influenced only by the sagittal skeletal pattern and was higher in Class III individuals.