bluuebunny/crossref_metadata_2025_split huggingface dataset:
CSRP2 is involved in immune regulation and drug sensitivity in glioma cells by the LEF1- AS1/miR-128-3p/CSRP2 axis
Authors: Zuo Wei , Jia Liyan , Chen Lin , Chen Xinlu , Yin Chonggao , Liu Wei , Li Hongli 
Published: March 2022

Abstract
Background: Glioma is one of the most common intracranial malignancies. As competing for endogenous RNA (ceRNA), long chain non-coding RNA (lncRNA) can affect the binding of microRNA (miRNA) to mRNA and thus affect the occurrence and development of glioma, which has been suggested as a key role in the development of human cancers. This study was to investigate the effects of TCGA on the development of glioma.Results: Here, we screened the differential genes by differential analyses on the matrix data for glioma in TCGA and GEO databases and selected the potential prognostic markers among them. Surprisingly, LEF1-AS1 was identified capable of influencing the survival of glioma patients. The results derived from the proportional risk (Cox) regression model showed a significant association between LEF1-AS1 overexpression and poorer prognosis (HR = 1.518, P < 0.001). The ROC model validated the feasibility of LEF1-AS1 (AUC = 0.702). MiR-128-3p, the target gene for LEF1-AS1, was screened using the Bioinformatics Online website and found down-regulated with increasing glioma grade in glioma tissues. Then the target gene for miR-128-3p, CSRP2, was obtained through miRTarBase, TargetScan, and miRDB. The survival curve of CSRP2 showed that CSRP2 with low expression corresponded to a better prognosis in glioma patients. The level of CSRP2 was positively correlated with the infiltration ones of CD4+ T and CD8+ T cells in glioma tissues. In addition, the CellMiner database predicted three drugs (Elesclomol, Entinostat, and Staurosporine) with high sensitivity to CSRP2 expression, and their molecular structures were retrieved using the PubChem22 database.Conclusions: In summary, the LEF1-AS1/miR-128-3p/CSRP2 axis may play a vital role in the development of glioma and provide guiding significance for the early diagnosis and treatment for it.
In silico and in vitro analysis of salt stress tolerance by Selenium application in proso millet
Authors: Mushtaq Naveed Ul , Saleem Seerat , Tahir Inayatullah , Rehman Reiaz Ul 
Published: March 2022

Abstract
Background and aimIn order to withstand abiotic stress conditions, plants accumulate a wide range of metabolic products such as proline and there exists a co-relation between proline accumulation and salt stress alleviation in plants, suggesting its role in stress mitigation and osmotic adjustments. MethodsIn this study we assessed the effect of Selenium (Se) under salt (NaCl) stress on Pyrroline-5-carboxylate synthase (P5CS), Proline dehydrogenase (ProDH) and Ornithine aminotransferase (OAT) proteins using in silico and in vitro studies. ResultsBased on in silico analysis it was found that these enzymes demonstrated a better interaction with Se in comparison to NaCl. The predicted results implied the merit of using Se as a NaCl mitigant. The morphological results revealed a decline in the growth parameters of plants under NaCl stress in a dose dependent manner. However, the application of Se relieved the NaCl induced symptoms. It was found that 1µM Se mitigated the NaCl stress effectively and there was an increase in proline content. The transcriptomic studies also showed that Se up-regulated the expression of P5CS, ProDH and OAT. Based on above results Se fulfills the criteria as an excellent mitigant for exogenous application in proso millet during NaCl stress.ConclusionUnder salt stress, Se at lower concentration (1µM) functions as a mitigant to alleviate NaCl stress by regulating the synthesis of proline. Se has high binding affinities with proline biosynthesis enzymes, increasing their activities and expression, enhancing proline content and growth in proso millet.
Carbon quantum dots from pomelo peel as fluorescence probes for “turn-off–on” high-sensitivity detection of Fe 3+ and L-cysteine
Authors: Zhang Dianwei , Zhang Furui , Liao Yonghong , Wang Fenghuan , Liu Huilin 
Published: March 2022

Abstract
This study designed a “turn-off–on” fluorescence analysis method based on carbon quantum dots (CQDs) to detect metal ions and amino acids in real sample systems. CQDs were derived from green pomelo peel via a one-step hydrothermal process. The co-doped CQDs with N, S atoms imparted excellent optical properties (quantum yield = 17.31%). The prepared CQDs could be used as fluorescent “turn-off” probes to detect Fe3+ with a limit of detection of 0.086 µM, a linear detection range of 0.1-160 µM, and recovery of 92.09%–104.87% in water samples. The quenched CQDs fluorescence could be turned on after adding L-cysteine (L-Cys), which allowed detection of L-Cys with a detection limit of 0.34 µM and linear range of 0.4–85 µM. Recovery of L-Cys in amino acid beverage was 92.58%–106.76%. Visual paper-based testing strips and cellulose/CQDs composite hydrogels could be also used to detect Fe3+ and L-Cys.
Identification and Validation of Real Hub Genes in Hepatocellular Carcinoma Based on Weighted Gene Co-expression Network Analysis
Authors: Zhao Zhigang , Qiao Yu , Wang Xin , Hu Jun , Mao Yurong , Yuan Fahu 
Published: March 2022

Abstract
Background: Hepatocellular Carcinoma (HCC) is one of the most common liver malignancies in the world. With highly invasive biological characteristics and lack of obvious clinical manifestations, hepatocellular Carcinoma usually has a poor prognosis and ranks fourth in cancer mortality. The aetiology and exact molecular mechanism of primary hepatocellular carcinoma are still unclear. This work aims to help identify biomarkers of early HCC diagnosis or prognosis. Results: In this study, Expression data and clinical information of HTSEQ-Counts were downloaded from The Cancer Genome Atlas (TCGA) database, and Gene Expression map GSE121248 was downloaded from Gene Expression Omnibus (GEO). By differentially expressed genes (DEGs) and Weighted Gene co-expression Network Analysis（WGCNA）searched for modules in the two databases that had the same effect on the biological characteristics of HCC, and extracted the module genes with the highest positive correlation with HCC from two databases, and finally obtained overlapping genes. Then, we performed functional enrichment analysis on the overlapping genes to understand their potential biological functions. The top ten hub genes were screened according to MCC through the String database and Cytoscape software. By survival analysis, high expression of CDK1, CCNA2, CDC20, KIF11, DLGAP5, KIF20A, ASPM, CEP55, TPX2 was associated with poorer overall survival (OS) of HCC patients. The DFS curve was plotted using the online website GEPIA2. Finally, based on the enrichment of these genes in the KEGG pathway, real hub genes were screened out, which were CDK1, CCNA2 and CDC20 respectively. Conclusions: High expression of these three genes was negatively correlated with survival time in HCC, and the expression of CDK1, CCNA2 and CDC20 were significantly higher in tumour tissues of HCC patients than in normal liver tissues as verified again by the HPA database. All in all, this provides a new feasible target for early and accurate diagnosis of HCC, clinical diagnosis, treatment and prognosis.
OAHEGA: From Research to Production of Mobile Expression Detection Application
Authors: Kovenko Volodymyr , Shevchuk Vitalii 
Published: March 2022

Abstract
Nowadays machine learning algorithms allow us to solve various difficult problems and optimize things a lot. One of such problems is detection of facial expressions. Detection of facial expressions can be used for market analysis, self-driving cars and in the entertainment industry. However, many challenges arise when trying to build a classifier for custom emotion, such as covariate shift and imbalanced number of instances per class. In this paper we present a new image dataset OAHEGA, that consists of six emotions, and conduct experiments on it. We also provide a comprehensive study of building a successful application on top of a model trained using the highlighted dataset.
Evaluating risk factors for cumulative life course impairment in psoriasis using Patient- Acceptable Symptom State and EQ-5D Running head: Risk factors for CLCI in psoriasis
Authors: Chularojanamontri Leena , Wongpraparut Chanisada , Silpa-archa Narumol , Chaiyabutr Chayada , Wongdama Supisara , Chiowchanwisawakit Praveena 
Published: March 2022

Abstract
The term “cumulative life course impairment” (CLCI) has been proposed to capture the overall effects of psoriasis that result in altered or impaired life potential during the life course of a patient. Severity and comorbidities were the most frequently studied risk factors for CLCI in psoriasis. This study evaluated the health-utility values and risk factors for CLCI using Patient Acceptable Symptom State (PASS) and EQ-5D. This cross-sectional investigation enrolled patients with psoriasis attending the Psoriasis Clinic, Siriraj Hospital, Bangkok. Patients were asked PASS questions about their overall self-perceived health state, adaptation, and expectations for current, future, and lifelong conditions. The patients also completed EQ-5D. The mean age of 139 enrolled patients was 45.8 ± 14.4 years, and 57.6% were women. There were 121 (87.1%) cases of chronic plaque psoriasis, 9 (6.5%) of guttate psoriasis, 4 (2.9%) of erythrodermic psoriasis, 3 (2.2%) of pustular psoriasis, and 2 (1.4%) of acrodermatitis continua of Hallopeau. For current PASS, satisfaction was significantly associated with older age, being married, and lower disease severity. The mean health-utility value and EQ-VAS score of the 139 patients were 0.89 ± 0.12 and 77.0 ± 17.4, respectively. Patients would not accept their disease if they had moderate to extreme problems in usual activities and depression/anxiety for the future and lifelong. Univariate analysis revealed that depression and usual activities were significantly associated with satisfaction for current PASS, future PASS, and lifelong PASS. In conclusion, disease severity, age, marital status, problems with usual activities, and depression/anxiety were significantly related to CLCI. These findings may allow physicians to identify psychosocial and psychological aspects of psoriatic patients at high risk for developing CLCI. Early and adequate treatment, good coping strategies, and good social support can prevent a negative impact on CLCI and major life-changing decisions.
Phase I trial of hypofractionated chemoradiotherapy in the palliative management of esophageal and gastro-esophageal cancer
Authors: Sridha Swetha , Day Fiona , Loh Jasmin , Lynam James , Smart Joanne , Holt Brandan , Mandaliya Hiren , Bonaventura Anthony , Kumar Mahesh , Martin Jarad 
Published: March 2022

Abstract
Background: Many patients with incurable esophageal cancer (ECa) present with dysphagia as their predominant symptom. Currently there is no consensus on how best to initially manage this scenario with multiple therapeutic options available. We aimed to assess the safety and efficacy of using hypofractionated radiotherapy given over a progressively shorter timeframe with concurrent carboplatin and paclitaxel in the management of patients with ECa and dysphagia. Methods: In this phase I trial we enrolled patients with histologically proven squamous cell carcinoma or adenocarcinoma of the esophagus or the gastro-esophageal junction with symptomatic dysphagia from local disease and not for curative treatment. Patients needed to be 18 years or older, have an ECOG performance status of 0-2 and be suitable to receive carboplatin and paclitaxel chemotherapy. Patients were placed in four progressively shorter radiation schedules culminating in 30Gy in 10 fractions in a step wise manner, all with concurrent carboplatin AUC 2 and paclitaxel 50mg/m2 chemotherapy delivered weekly with the radiation therapy. The primary endpoint was the development of the dose limiting toxicities (DLTs) esophageal perforation or febrile neutropenia. Secondary endpoints were relief of dysphagia, time to improvement of dysphagia, dysphagia progression free survival and overall survival. Results: Eighteen patients were enrolled in the study between October 2014 and March 2019. There were no DLTs experienced during the trial. The most common grade 3+ acute toxicity experienced by patients were nausea and vomiting (both in 4/18 patients). The most common radiation specific acute toxicity experienced was esophagitis with 67% of patients experiencing grade 1-2 symptoms. All patients experienced improvement in dysphagia. The median time to dysphagia improvement was three weeks from the start of chemoradiotherapy (CTRT) (range 2-10 weeks). The median dysphagia free survival was 5.8 months with a median overall survival of 8.9 months. Conclusion: Hypofractionated palliative CTRT with 30Gy/10# of radiation therapy with concurrent weekly carboplatin and paclitaxel chemotherapy is well tolerated and provides a good response in improvement of dysphagia. Further studies need to be undertaken which provide both symptomatic improvement in the primary tumor but also control of the metastatic burden in these patients.Clinical Trial Registration: This trial was prospectively registered with www.anzctr.org.au Identifier: ACTRN12614000821695
Helical versus static approaches to delivering tomotherapy to the junctional target for patients taller than 135 cm undergoing total body irradiation
Authors: Köksal Mümtaz , Baumert Jonathan , Schoroth Felix , Müdder Thomas , Scafa Davide , Koch David , Leitzen Christina , Sarria Gustavo , Schmeel Leonard , Giordano Frank 
Published: March 2022

Abstract
Background: Helical TomoTherapy® is widely used for total body irradiation as a component of conditioning regimens before allogeneic bone marrow transplantation. However, this technique limits the maximum length of a planning target volume to 135 cm. Therefore, patients taller than 135 cm require two planning computed tomography scans and treatment plans. The junctional target between these two treatment plans is thus a critical region for treatment planning and delivery. Here, we compare radiation coverage of the junctional target between helical and static approaches to treatment planning and delivery to determine which approach allows high quality irradiation planning and provides more robustness against patient movement.Methods: We retrospectively analyzed 10 patients who underwent total body irradiation using a static four-field box planning approach and nine patients who underwent total body irradiation using a helical planning approach. All patients were taller than 135 cm. The junctional target volume was divided into 10 slices of 1-cm thickness (JT1-JT10) for analysis. Dosimetric parameters and dose-volume histograms were compared to assess the quality of coverage of the junctional target between the helical and static planning approaches.Results: The D50 for the total junctional target was slightly higher than the prescribed dose for both helical and static approaches, with a mean of 108.12% for the helical group and 107.81% for the static group. The mean D95 was 98.44% ± 4.19% for the helical group and 96.20% ± 4.59% for the static group. The mean homogeneity index covering the entire junctional target volume was 1.20 ± 0.04 for the helical group and 1.21 ± 0.05 for the static group. The mean homogeneity index ranged from 1.08 ± 0.01 in JT1 to 1.22 ± 0.06 in JT6 for the helical group and from 1.06 ± 0.02 in JT1 to 1.19 ± 0.05 in JT6 for the static group. There were no significant differences in parameters between helical and static groups. However, the static approach provided robustness against up to 30 mm of lateral movement of the patient.Conclusions: As long as TBI using helical TomoTherapy® is limited to a maximum length of 135 cm, the junctional target must be addressed during treatment planning. Our analysis shows that the static four-field box approach is viable and offers higher robustness against lateral movement of the patient than the helical approach.
Studies on structural and electrical properties of NaI doped PEO/CMC blend solid polymer electrolyte
Authors: N NAGAIAH , Samartharama B N , Ambika M R , Demappa T 
Published: March 2022

Abstract
Solid polymer blend electrolyte films of PEO/Na-CMC doped with NaI, were prepared by solution cast method and their structural, optical and electrical properties were investigated using X-Ray diffraction, Fourier transform infrared spectroscopy, UV-Vis spectrophotometer and Electrical impedance analyser respectively. X-Ray diffraction spectra indicate that, the deformation occurred in the structure of the blend followed by doping with NaI salt. The variation in intensity and shifting of characteristic bands in FTIR spectra provides the evidence for formation of complex due to the interaction occurred in the blend with the dopant. Optical energy band gap decreases in the blend samples upon doping and is confirmed by UV-Vis spectrophotometer analysis. Increase of Dielectric constant and loss upon doping, exhibits usual behaviour of the polymer electrolytes. The nature of increase in AC conductivity by doping and maximum conductivity obtained for 10/90 sample doped with 0.03% NaI indicates, the prepared sample can be used as polymer electrolytes in the manufacturing of energy storage devices.
Improved the methanol electrooxidation and carbon monoxide tolerance for direct methanol fuel cells using strontium molybdate
Authors: Chiang Tzu Hsuan , Hsu Jia-Wei 
Published: March 2022

Abstract
Electrocatalysts possess high methanol electrooxidation (MOR) and carbon monoxide (CO) tolerance abilities to meet application requirements for enhanced performance of direct methanol fuel cells (DMFCs). Higher MOR and CO tolerance activities measured via cyclic voltammetry (CV) are achieved based on a strontium molybdate (SrMoO4) mixed with Vulcan XC-72 carbon and loaded with 20% Pt. The synergistic effect of the bifunctional mechanism inducing the strontium molybdate is beneficial for removing CO-like intermediate products on the Pt surface, which leads to more Pt active sites released during MOR. The simultaneous unique structural formation of HxMoO3/HyMoO3 and SrMoO3 in uncalcined Sr0.5Mo0.5O4−δ provides key synergistic effects for the 20%-Pt/uncalcined Sr0.5Mo0.5O4−δ-C electrocatalysts, improving DMFCs performance. Results show that the 20%-Pt/uncalcined Sr0.5Mo0.5O4−δ-C electrocatalysts exhibit excellent electrocatalytic activity for MOR (with a forward peak current density of 12.56 mA/cm2 and large electrochemically active surface area (ECSAH) of 116.53 m2/g, the best CO tolerance for electrooxidation of carbon monoxide (largest ECSACO of 116.53 m2/g), and the highest electric conductivity (Rct of 940 Ω∙cm2). Furthermore, the fabricated DMFC shows excellent long-term electrochemical stability after 1000 cycles and a maximum power density (1.42 mW/cm2) higher than that with commercial 20%-Pt/C (1.27 mW/cm2).
YY1 Promotes Pancreatic Cancer Cell proliferation by Enhancing Mitochondrial Respiration
Authors: Jiang Minghua , Li Bin , Wang Junyi , Liao Jing , Wu Minghui , Yuan Xiangshu , Fang Hezhi , Shen Lijun 
Published: March 2022

Abstract
KRAS-driven metabolic reprogramming is one of the key features of pancreatic ductal adenocarcinoma (PDAC) cells; however, the metabolic roles of other oncogenic genes, such as YY1, in PDAC development are still unclear. In this study, we observed that YY1 expression was significantly elevated in human PDAC tissues and was positively correlated with a poor disease progression. Further, in vitro studies confirmed that YY1 deletion inhibited PDAC cell proliferation and tumorigenicity. Moreover, YY1 deletion also led to impaired mitochondrial RNA expression, which further induced mitochondrial oxidative phosphorylation (OXPHOS) complex assembly deficiency and altered cellular nucleotide homeostasis. Mechanistically, the impairment of mitochondrial OXPHOS function lowered the generation of aspartate, an output of the tricarboxylic acid cycle (TCA) and resulted in the inhibition of cell proliferation, owing to the absence of aspartate-associated nucleotide availability. Conversely, exogenous supplementation with aspartate fully restored PDAC cell proliferation. Altogether, our findings suggested that YY1 promotes PDAC cell proliferation by enhancing mitochondrial respiration and the TCA, which favors aspartate-associated nucleotide synthesis. These findings indicate that targeting nucleotide biosynthesis is a promising strategy for PDAC treatment.
Matrix Stiffness and Architecture Drive Fibro-adipogenic Progenitors’ Activation into Myofibroblasts
Authors: Loomis Taryn , Hu Lin-Ya , Wohlgemuth Ross P. , Chellakudam Rosemary R. , Smith Lucas R. 
Published: March 2022

Abstract
BackgroundFibro-adipogenic progenitors (FAPs) are essential in supporting regeneration in skeletal muscle but pathologically become the main source of excess extracellular matrix (ECM) deposition in fibrosis. Fibrosis can create a progressively degenerative feedback loop with fibrotic tissue leading to an increase in the fibrotic FAP phenotype. Fibrotic ECM has altered mechanical and architectural properties, but the interactions between FAPs and the ECM are not well understood.MethodsIn this study, we aimed to examine FAPs’ sensitivity to their environment and its effect on cell fate. Polyacrylamide and collagen hydrogels were utilized to investigate substrate stiffness and ECM architecture. Collagen hydrogels were manipulated to mimic aspects of healthy and fibrotic ECM including density, crosslinking, fibril size, and alignment. Collagen-coated polyacrylamide gels were utilized to analyze substrate stiffnesses that are physiologically relevant. YAP nuclear localization, proliferation, and myofibroblast activation were measured using immunofluorescence and custom FIJI: ImageJ scripts. Results Substrates of fibrotic stiffnesses had increased myofibroblast activation compared to substrates mimicking healthy muscle. The increase in myofibroblast activation correlated strongly with in Yes-associated protein (YAP) nuclear localization. Collagen crosslinking and increased fibril size inhibited myofibroblast activation, independent of YAP localization. Alignment of collagen did not affect FAP behavior.ConclusionsThis study shows that FAPs possess the ability to sense their ECM environment and that influences cell fate. Therefore, ECM mechanics and architecture are key targets to reduce the progression of fibrosis.
The 3a protein of SARS-CoV-2 is a potential calcium binding protein
Authors: Minakshi Rinki , Malik Zubbair , Rahman Safikur 
Published: March 2022

Abstract
The 3a protein is an accessory protein of SARS-CoV-2. We present here the result of bioinformatics analysis based on cryo-EM structure of 3a protein that has predicted calcium binding, majorly in cytoplasmic domain of 3a protein, thereby providing crucial information for further understanding of viral pathogenesis in context to its effect on cellular calcium signaling. The prediction analysis has also shown binding of antiviral drug amantadine with 3a protein of SARS-CoV-2.