bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antimicrobial resistance and population genomics of multidrug-resistant Escherichia coli in pig farms in mainland China
Authors: Peng Zhong , Hu Zizhe , Li Zugang , Zhang Xiaoxue , Jia Chaoying , Li Tianzhi , Dai Menghong , Tan Chen , Xu Zhuofei , Wu Bin , Chen Huanchun , Wang Xiangru 
Published: March 2022

AbstractThe expanding use of antimicrobials in livestock is an important contributor to the worldwide rapid increase in antimicrobial resistance (AMR). However, large-scale studies on AMR in livestock remain scarce. Here, we report findings from surveillance of E. coli AMR in pig farms in China in 2018–2019. We isolated E. coli in 1,871 samples from pigs and their breeding environments, and found AMR in E. coli in all provinces in mainland China. We detected multidrug-resistance in 91% isolates and found resistance to last-resort drugs including colistin, carbapenems and tigecycline. We also identified a heterogeneous group of O-serogroups and sequence types among the multidrug-resistant isolates. These isolates harbored multiple resistance genes, virulence factor-encoding genes, and putative plasmids. Our data will help to understand the current AMR profiles of pigs and provide a reference for AMR control policy formulation for livestock in China.
Pushing detectability and sensitivity for subtle force to new limits with shrinkable nanochannel structured aerogel
Authors: Shi Xinlei , Fan Xiangqian , Zhu Yinbo , Liu Yang , Wu Peiqi , Jiang Renhui , Wu Bao , Wu Heng-An , Zheng He , Wang Jianbo , Ji Xinyi , Chen Yongsheng , Liang Jiajie 
Published: March 2022

AbstractThere is an urgent need for developing electromechanical sensor with both ultralow detection limits and ultrahigh sensitivity to promote the progress of intelligent technology. Here we propose a strategy for fabricating a soft polysiloxane crosslinked MXene aerogel with multilevel nanochannels inside its cellular walls for ultrasensitive pressure detection. The easily shrinkable nanochannels and optimized material synergism endow the piezoresistive aerogel with an ultralow Young’s modulus (140 Pa), numerous variable conductive pathways, and mechanical robustness. This aerogel can detect extremely subtle pressure signals of 0.0063 Pa, deliver a high pressure sensitivity over 1900 kPa−1, and exhibit extraordinarily sensing robustness. These sensing properties make the MXene aerogel feasible for monitoring ultra-weak force signals arising from a human’s deep-lying internal jugular venous pulses in a non-invasive manner, detecting the dynamic impacts associated with the landing and take-off of a mosquito, and performing static pressure mapping of a hair.
ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer’s disease models
Authors: Zhao Yuanyuan , Hu Di , Wang Rihua , Sun Xiaoyan , Ropelewski Philip , Hubler Zita , Lundberg Kathleen , Wang Quanqiu , Adams Drew J. , Xu Rong , Qi Xin 
Published: March 2022

AbstractPredisposition to Alzheimer’s disease (AD) may arise from lipid metabolism perturbation, however, the underlying mechanism remains elusive. Here, we identify ATPase family AAA-domain containing protein 3A (ATAD3A), a mitochondrial AAA-ATPase, as a molecular switch that links cholesterol metabolism impairment to AD phenotypes. In neuronal models of AD, the 5XFAD mouse model and post-mortem AD brains, ATAD3A is oligomerized and accumulated at the mitochondria-associated ER membranes (MAMs), where it induces cholesterol accumulation by inhibiting gene expression of CYP46A1, an enzyme governing brain cholesterol clearance. ATAD3A and CYP46A1 cooperate to promote APP processing and synaptic loss. Suppressing ATAD3A oligomerization by heterozygous ATAD3A knockout or pharmacological inhibition with DA1 restores neuronal CYP46A1 levels, normalizes brain cholesterol turnover and MAM integrity, suppresses APP processing and synaptic loss, and consequently reduces AD neuropathology and cognitive deficits in AD transgenic mice. These findings reveal a role for ATAD3A oligomerization in AD pathogenesis and suggest ATAD3A as a potential therapeutic target for AD.
Improving the oxygen redox reversibility of Li-rich battery cathode materials via Coulombic repulsive interactions strategy
Authors: Li Qingyuan , Ning De , Wong Deniz , An Ke , Tang Yuxin , Zhou Dong , Schuck Götz , Chen Zhenhua , Zhang Nian , Liu Xiangfeng 
Published: March 2022

AbstractThe oxygen redox reaction in lithium-rich layered oxide battery cathode materials generates extra capacity at high cell voltages (i.e., >4.5 V). However, the irreversible oxygen release causes transition metal (TM) dissolution, migration and cell voltage decay. To circumvent these issues, we introduce a strategy for tuning the Coulombic interactions in a model Li-rich positive electrode active material, i.e., Li1.2Mn0.6Ni0.2O2. In particular, we tune the Coulombic repulsive interactions to obtain an adaptable crystal structure that enables the reversible distortion of TMO6 octahedron and mitigates TM dissolution and migration. Moreover, this strategy hinders the irreversible release of oxygen and other parasitic reactions (e.g., electrolyte decomposition) commonly occurring at high voltages. When tested in non-aqueous coin cell configuration, the modified Li-rich cathode material, combined with a Li metal anode, enables a stable cell discharge capacity of about 240 mAh g−1 for 120 cycles at 50 mA g−1 and a slower voltage decay compared to the unmodified Li1.2Mn0.6Ni0.2O2.
Acute kidney rejection after anti-SARS-CoV-2 virus-vectored vaccine—case report
Authors: Vnučák Matej , Graňák Karol , Beliančinová Monika , Jeseňák Miloš , Macháleková Katarína Kajová , Benko Jakub , Samoš Matej , Dedinská Ivana 
Published: March 2022

AbstractCOVID-19 infection remains a threat to the health systems of many countries. Potential success in the fight against the COVID-19 pandemic is the vaccination of high-risk groups, including patients with end-stage kidney disease (ESKD) and after solid organ transplantation (SOT). Immunosuppression in kidney transplant recipients can also reduce the immunogenicity of SARS-CoV-2 vaccines (varied by vaccine platform), available data suggest that they are efficacious in approximately 50–70%, compared to non-transplant situations. In this paper, we present a newly developed acute humoral and cellular rejection with acute allograft failure and need of hemodialysis 14 days after administration of the adenovirus vectored SARS-CoV-2 vaccine (AstraZeneca; CHADOx1, AZD1222). This occurred in a patient who previously had an asymptomatic COVID-19 infection. Case reports of acute allograft rejection after vaccination against SARS-CoV-2 can help stratify risk groups of patients who develop hyperimmune reactions. However, it is also possible that those with a previous mild primary COVID-19 infection may also develop acute allograft rejections upon COVID-19 re-infection.
Data on the diets of Salish Sea harbour seals from DNA metabarcoding
Authors: Thomas Austen C. , Deagle Bruce , Nordstrom Chad , Majewski Sheena , Nelson Benjamin W. , Acevedo-Gutiérrez Alejandro , Jeffries Steven , Moore Jed , Louden Amelia , Allegue Hassen , Pearson Scott , Schmidt Michael , Trites Andrew W. 
Published: March 2022

AbstractMarine trophic ecology data are in high demand as natural resource agencies increasingly adopt ecosystem-based management strategies that account for complex species interactions. Harbour seal (Phoca vitulina) diet data are of particular interest because the species is an abundant predator in the northeast Pacific Ocean and Salish Sea ecosystem that consumes Pacific salmon (Oncorhynchus spp.). A multi-agency effort was therefore undertaken to produce harbour seal diet data on an ecosystem scale using, 1) a standardized set of scat collection and analysis methods, and 2) a newly developed DNA metabarcoding diet analysis technique designed to identify prey species and quantify their relative proportions in seal diets. The DNA-based dataset described herein contains records from 4,625 harbour seal scats representing 52 haulout sites, 7 years, 12 calendar months, and a total of 11,641 prey identifications. Prey morphological hard parts analyses were conducted alongside, resulting in corresponding hard parts data for 92% of the scat DNA samples. A custom-built prey DNA sequence database containing 201 species (192 fishes, 9 cephalopods) is also provided.
Dataset of ultralow temperature refrigeration for COVID 19 vaccine distribution solution
Authors: Sun Jian , Zhang Mingkan , Gehl Anthony , Fricke Brian , Nawaz Kashif , Gluesenkamp Kyle , Shen Bo , Munk Jeff , Hagerman Joe , Lapsa Melissa , Awwad Nader , Recipe Chris , Auyer Doug , Brisson David 
Published: March 2022

AbstractMost COVID-19 vaccines require temperature control for transportation and storage. Two types of vaccine have been developed by manufacturers (Pfizer and Moderna). Both vaccines are based on mRNA and lipid nanoparticles requiring low temperature storage. The Pfizer vaccine requires ultra-low temperature storage (−80 °C to −60 °C), while the Moderna vaccine requires −30 °C storage. However, the last stage of distribution is quite challenging, especially for rural or suburban areas, where local towns, pharmacy chains and hospitals may not have the infrastructure required to store the vaccine at the required temperature. In addition, there is limited data available to address ancillary challenges of the distribution framework for both transportation and storage stages, including safety concerns due to human exposure to large amounts of CO2 from dry-ice sublimation, issues due to the pressure increase caused by dry-ice sublimation, and the potential issue caused by non-uniform cryogenic temperatures. As such, there is a need for test dataset to assist the development of a quick, effective, secure, and safe solution to mitigate the challenges faced by vaccine distribution logistics.
Validation and refinement of cropland data layer using a spatial-temporal decision tree algorithm
Authors: Lin Li , Di Liping , Zhang Chen , Guo Liying , Di Yahui , Li Hui , Yang Anna 
Published: March 2022

AbstractSpace-based crop identification and acreage estimation have played a significant role in agricultural studies in recent years, due to the development of Remote Sensing technology. The Cropland Data Layer (CDL), which was developed by the U.S. Department of Agriculture (USDA), has been widely used in agricultural studies and achieved massive success in recent years. Although the CDL’s accuracy assessments report high overall accuracy on various crops classifications, misclassification is still common and easy to discern from visual inspection. This study is aimed to identify and resolve inaccurate crop classification in CDL. A decision tree method was employed to find questionable pixels and refine them with spatial and temporal crop information. The refined data was then evaluated with high-resolution satellite images and official acreage estimates from USDA. Two validation experiments were also developed to examine the data at both the pixel and county level. Data generated from this research was published online in two repositories, while both applications allow users to download the entire dataset at no cost.
Investigating adult age differences in real-life empathy, prosociality, and well-being using experience sampling
Authors: Pollerhoff Lena , Stietz Julia , Depow Gregory John , Inzlicht Michael , Kanske Philipp , Li Shu-Chen , Reiter Andrea M. F. 
Published: March 2022

AbstractWhile the importance of social affect and cognition is indisputable throughout the adult lifespan, findings of how empathy and prosociality develop and interact across adulthood are mixed and real-life data are scarce. Research using ecological momentary assessment recently demonstrated that adults commonly experience empathy in daily life. Furthermore, experiencing empathy was linked to higher prosocial behavior and subjective well-being. However, to date, it is not clear whether there are adult age differences in daily empathy and daily prosociality and whether age moderates the relationship between empathy and prosociality across adulthood. Here we analyzed experience-sampling data collected from participants across the adult lifespan to study age effects on empathy, prosocial behavior, and well-being under real-life circumstances. Linear and quadratic age effects were found for the experience of empathy, with increased empathy across the three younger age groups (18 to 45 years) and a slight decrease in the oldest group (55 years and older). Neither prosocial behavior nor well-being showed significant age-related differences. We discuss these findings with respect to (partially discrepant) results derived from lab-based and traditional survey studies. We conclude that studies linking in-lab experiments with real-life experience-sampling may be a promising venue for future lifespan studies.
Influence of heat flow control on dynamical spin injection in CoFeB/Pt/CoFeB trilayer
Authors: Obinata Sora , Iimori Riku , Ohnishi Kohei , Kimura Takashi 
Published: March 2022

AbstractA dynamical spin injection based on the ferromagnetic resonance in a ferromagnetic/nonmagnetic bi-layered structure, is a powerful mean for generating and manipulating the spin current. Although the mechanism of the dynamical spin injection is mainly attributed to the spin pumping, the detailed mechanism and the quantitative understanding for related phenomena are still controversial. As an another important contribution to the dynamical spin injection, the heating effect due to the resonant precessional motion of the magnetization is pointed out recently. In order to quantify the contribution from the heating effect, we here investigate the dynamical spin injection in a CoFeB/Pt/CoFeB trilayer. Although the contribution from the spin pumping diminishes because of the symmetric spin injection from the upper and lower interfaces, a significant inverse spin Hall voltage has been clearly observed. We show that the observed voltage can be quantitatively understood by the thermal spin injection due to a heating effect during the ferromagnetic resonance. A proper combination between the spin pumping and the heat-flow control in the multi-layered system is a key for the efficient dynamical spin injection.
From Khoi-San indigenous knowledge to bioengineered CeO2 nanocrystals to exceptional UV-blocking green nanocosmetics
Authors: Ditlopo N. , Sintwa N. , Khamlich S. , Manikandan E. , Gnanasekaran K. , Henini M. , Gibaud A. , Krief A. , Maaza M. 
Published: March 2022

AbstractSingle phase CeO2 nanocrystals were bio-synthesized using Hoodia gordonii natural extract as an effective chelating agent. The nanocrystals with an average diameter of 〈Ø〉 ~ 5–26 nm with 4+ electronic valence of Ce displayed a remarkable UV selectivity and an exceptional photostability. The diffuse reflectivity profile of such CeO2 exhibited a unique UV selectivity, in a form of a Heaviside function-like type profile in the solar spectrum. While the UV reflectivity is significantly low; within the range of 0.7%, it reaches 63% in the VIS and NIR. Their relative Reactive Oxygen Species (ROS) production was found to be < 1 within a wide range of concentration (0.5–1000 μg/ml). This exceptional photostability conjugated to a sound UV selectivity opens a potential horizon to a novel family of green nano-cosmetics by green nano-processing.
Genetically related genotypes of cowpea present similar bacterial community in the rhizosphere
Authors: de Albuquerque Tayná Mendes , Mendes Lucas William , Rocha Sandra Mara Barbosa , Antunes Jadson Emanuel Lopes , Oliveira Louise Melo de Souza , Melo Vania Maria Maciel , Oliveira Francisca Andrea Silva , Pereira Arthur Prudêncio de Araujo , da Silva Veronica Brito , Gomes Regina Lucia Ferreira , de Alcantara Neto Francisco , Lopes Angela Celis de Almeida , de Moura Rocha Maurisrael , Araujo Ademir Sergio Ferreira 
Published: March 2022

AbstractPlant breeding reduces the genetic diversity of plants and could influence the composition, structure, and diversity of the rhizosphere microbiome, selecting more homogeneous and specialized microbes. In this study, we used 16S rRNA sequencing to assess the bacterial community in the rhizosphere of different lines and modern cowpea cultivars, to investigate the effect of cowpea breeding on bacterial community assembly. Thus, two African lines (IT85F-2687 and IT82D-60) and two Brazilian cultivars (BRS-Guariba and BRS-Tumucumaque) of cowpea were assessed to verify if the generation advance and genetic breeding influence the bacterial community in the rhizosphere. No significant differences were found in the structure, richness, and diversity of bacterial community structure between the rhizosphere of the different cowpea genotypes, and only slight differences were found at the OTU level. The complexity of the co-occurrence network decreased from African lines to Brazilian cultivars. Regarding functional prediction, the core functions were significantly altered according to the genotypes. In general, African lines presented a more abundance of groups related to chemoheterotrophy, while the rhizosphere of the modern cultivars decreased functions related to cellulolysis. This study showed that the genetic breeding process affects the dynamics of the rhizosphere community, decreasing the complexity of interaction in one cultivar. As these cowpea genotypes are genetically related, it could suggest a new hypothesis of how genetic breeding of similar genotypes could influence the rhizosphere microbiome.
Determination, residue analysis and risk assessment of thiacloprid and spirotetramat in cowpeas under field conditions
Authors: Li Kailong , Chen Wuying , Xiang Wei , Chen Tongqiang , Zhang Min , Ning Ying , Liu Yong , Chen Ang 
Published: March 2022

AbstractThe dissipation and residue levels of thiacloprid, spirotetramat and its four metabolites residues in cowpeas were investigated under field conditions. The QuEChERS technique with high-performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS) was used to detect thiacloprid, spirotetramat and its four metabolites residues content in cowpeas. The recoveries were 81.3–95.1% at a spike level of 0.005–0.5 mg/kg, the relative standard deviations (RSDs) were 2.1–9.5%. The dissipation kinetics data showed that thiacloprid and spirotetramat in cowpeas were degraded with the half-lives of 1.14–1.54 days and 1.25–2.79 days. The terminal residues of thiacloprid and spirotetramat were 0.0255–0.4570 mg kg−1 and 0.0314–0.3070 mg kg−1 after application 2 times with a pre-harvest interval (PHI) of 3 days under the designed dosages. The chronic and acute dietary exposure assessment risk quotient (RQ) values of thiacloprid in cowpeas for different consumers were 2.44–4.41% and 8.72–15.78%, respectively, and those of spirotetramat were 1.03–1.87% and 0.18–0.32%, respectively, all of the RQ values were lower than 100%. The dietary risk of thiacloprid through cowpeas to consumers was higher than spirotetramat. The results from this study are important reference for Chinese governments to develop criteria for the safe and rational use of thiacloprid and spirotetramat, setting maximum residue levels (MRLs), monitoring the quality safety of agricultural products and protecting consumer health.
Molecular phylogenetic and morphometric analysis of population structure and demography of endangered threadfin fish Eleutheronema from Indo-Pacific waters
Authors: Xiao Jie , Lyu Shaoliang , Iqbal Teuku H. , Hajisamae Sukree , Tsim Karl W. K. , Wang Wen-Xiong 
Published: March 2022

AbstractThe threadfin Eleutheronema are the important fishery resources in Indo-Pacific regions and classified as the endangered species with considerable conservation values. Their genetic diversity and population structure remain essentially unknown but are critical for the proper management and sustainable harvests of such important fisheries. Here, the mitochondrial DNA sequences of CO1 and 16s rRNA were determined from 75 individuals of Eleutheronema tetradactylum and 89 individuals of Eleutheronema rhadinum collected from different locations of South China Sea and Thailand coastal waters. Genetic diversity analysis revealed that both E. tetradactylum (Haplotype diversity, H = 0.105–0.211; Nucleotide diversity, π = 0.00017–0.00043) and E. rhadinum (H = 0.074–0.663, π = 0.00013–0.01088) had low diversity. Population structure analysis demonstrated the shallow genetic differentiation among the South China Sea populations. The limited communication between China and Thailand populations caused the high genetic differentiation in all groups due to the low dispersal ability. Reconstruction of CO1 phylogenetic relationships and demographic studies across Indo-West-Pacific regions provided strong evidence for a shared common origin or ancestor of E. tetradactylum and E. rhadinum. Eleutheronema rhadinum were further subdivided into two distinct genetic lineages, with Clade A dominantly distributing in Thailand and Malaysia and Clade B distributing in China coastal waters. Phenotypic divergence, characterized mainly by the depth of caudal peduncle and length of caudal peduncle, was also observed for all populations, which was possibly associated with specific local adaptations to environmental changes. Our study suggested a strong need for the development of proper fishery management strategies and conservation actions for the imperiled Eleutheronema species.
3D quasi-skyrmions in thick cylindrical and dome-shape soft nanodots
Authors: Berganza Eider , Fernandez-Roldan Jose Angel , Jaafar Miriam , Asenjo Agustina , Guslienko Konstantin , Chubykalo-Fesenko Oksana 
Published: March 2022

AbstractMagnetic skyrmions are widely attracting researchers due to fascinating physics and novel applications related to their non-trivial topology. Néel skyrmions have been extensively investigated in magnetic systems with Dzyaloshinskii–Moriya interaction (DMI) and/or perpendicular magnetic anisotropy. Here, by means of micromagnetic simulations and analytical calculations, we show that 3D quasi-skyrmions of Néel type, with topological charge close to 1, can exist as metastable states in soft magnetic nanostructures with no DMI, such as in Permalloy thick cylindrical and dome-shaped nanodots. The key factor responsible for the stabilization of DMI-free is the interplay of the exchange and magnetostatic energies in the nanodots. The range of geometrical parameters where the skyrmions are found is wider in magnetic dome-shape nanodots than in their cylindrical counterparts. Our results open the door for a new research line related to the nucleation and stabilization of magnetic skyrmions in a broad class of nanostructured soft magnetic materials.
Development of a one-step analysis method for several amino acids using a microfluidic paper-based analytical device
Authors: Kugimiya Akimitsu , Wakimoto Sho , Kohda Jiro , Nakano Yasuhisa , Takano Yu 
Published: March 2022

AbstractA one-step analysis method was developed for four types of amino acids using a microfluidic paper-based analytical device fabricated from chromatography filtration paper and laminate films. Aminoacyl-tRNA synthetase was used to detect each amino acid. The obtained laminated paper-based analytical device (LPAD) contained four enzymatic reaction areas. Colorimetric detection was performed based on the molybdenum blue reaction. A model method for the simple, easy, and simultaneous detection of several amino acid concentrations was suggested, in contrast to the conventional methods such as HPLC or LC–MS. The method provided a selective quantification at the ranges of 3.6–100 μM for tryptophan, 10.1–100 μM for glycine, 5.9–100 μM for histidine and 5.6–100 μM for lysine with a detection limit of 1.1 μM, 3.3 μM, 1.9 μM and 1.8 μM, respectively. LPAD fabrication was considerably simple, and the subsequent detection process was easy and required a short period of time (within 15 min).