bluuebunny/crossref_metadata_2025_split huggingface dataset:
Canine saliva is a source of interspecies antimicrobial resistance gene transfer
Authors: Tóth Adrienn Gréta , Tóth Imre , Rózsa Bernadett , Kovács Eszter Gabriella , Dubecz Attila , Patai Árpád V. , Németh Tibor , Kaplan Selçuk , Makrai László , Solymosi Norbert 
Published: March 2022

ABSTRACTWhile the One Health issues of intensive animal farming are commonly discussed, keeping companion animals is less associated with the interspecies headway of antimicrobial resistance. With the constant advance of veterinary standards, antibiotics are regularly applied in companion animal medicine. Due to the close coexsistance of dogs and humans, dog bites and other casual encounters with dog saliva (e.g. licking the owner) are common. According to our metagenome studies based on 26 new generation sequencing canine saliva datasets from 2020 and 2021 reposited in NCBI SRA by The 10,000 Dog Genome Consortium and the Broad Institute within Darwin’s Ark project, canine saliva is rich in bacteria with predictably transferable antimicrobial resistance genes (ARGs). In the genom of potentially pathogenic Bacteroides, Capnocytophaga, Corynebacterium, Fusobacterium, Pasteurella, Porphyromonas, Staphylococcus and Streptococcus species, that are some of the most relevant bacteria in dog bite infections, ARGs against aminoglycosides, carbapenems, cephalosporins, glycylcyclines, lincosamides, macrolides, oxazolidinone, penams, phenicols, pleuromutilins, streptogramins, sulfonamides and tetracyclines could be identfied. Several ARGs, including ones against amoxicillin-clavulanate, the most commonly applied antibiotic by dog bites, was predicted to be potentially transferable based on their association with mobile genetic elements (e.g. plasmids, phages, integrated mobile genetic elements). According to our findings canine saliva may be a source of transfer of ARG-rich bacteria, that can either colonize the human body or transport ARGs to the host bacteriota and thus can be considered as a risk in the spread of antimicrobial resistance.
Heterotrimeric G proteins regulate planarian regeneration and behavior
Authors: Jenkins Jennifer E. , Roberts-Galbraith Rachel H. 
Published: March 2022

AbstractG protein-coupled receptors (GPCRs) play broad roles in development and stem cell biology, but few roles for GPCR signaling in complex tissue regeneration have been uncovered. Planarian flatworms robustly regenerate all tissues and provide a model with which to explore potential functions for GPCR signaling in somatic regeneration and pluripotent stem cell biology. As a first step toward exploring GPCR function in planarians, we investigated downstream signal transducers that work with GPCRs, called heterotrimeric G proteins. Here, we characterized the complete heterotrimeric G protein complement in Schmidtea mediterranea for the first time and found that seven heterotrimeric G protein subunits promote regeneration. We further characterized two subunits critical for regeneration, Gαq1 and Gβ1-4a, finding that they promote the late phase of anterior polarity re-establishment, likely through anterior pole-produced Follistatin. Incidentally, we also found that five heterotrimeric G proteins modulate planarian behavior. We further identified a putative serotonin receptor, gcr052, that we propose works with Gβx2 in planarian locomotion, demonstrating the utility of our strategy for identifying relevant GPCRs. Our work provides foundational insight into roles of heterotrimeric G proteins in planarian biology and serves as a useful springboard towards broadening our understanding of GPCR signaling in adult tissue regeneration.
A hypomorphic mutation in Pold1 disrupts the coordination of embryo size expansion and morphogenesis during gastrulation
Authors: Chen Tingxu , Alcorn Heather , Devbhandari Sujan , Remus Dirk , Lacy Elizabeth , Huangfu Danwei , Anderson Kathryn V. 
Published: March 2022

AbstractFormation of a properly sized and patterned embryo during gastrulation requires a well-coordinated interplay between cell proliferation, lineage specification and tissue morphogenesis. Following transient physical or pharmacological manipulations, pre-gastrulation stage mouse embryos show remarkable plasticity to recover and resume normal development. However, it remains unclear how mechanisms driving lineage specification and morphogenesis respond to defects in cell proliferation during and after gastrulation. Null mutations in DNA replication or cell-cycle related genes frequently lead to cell cycle arrest and reduced cell proliferation, resulting in developmental arrest before the onset of gastrulation; such early lethality precludes studies aiming to determine the impact of cell proliferation on lineage specification and morphogenesis during gastrulation. From an unbiased ENU mutagenesis screen, we discovered a mouse mutant, tiny siren (tyrn), that carries a hypomorphic mutation producing an aspartate to tyrosine (D939Y) substitution in Pold1, the catalytic subunit of DNA polymerase δ. Impaired cell proliferation in the tyrn mutant leaves anterior-posterior patterning unperturbed during gastrulation but results in an overall reduction in embryo size and in severe morphogenetic defects. Our analyses show that the successful execution of morphogenetic events during gastrulation requires that lineage specification and the ordered production of differentiated cell types occur in concordance with embryonic growth.Summary statementPold1 hypomorphic mutation caused reduced size and abnormal morphology of gastrulating mouse embryos, supporting the importance of coordinated embryo size, lineage specification and tissue morphogenesis for normal embryogenesis.
Simultaneous dual recordings from vestibular hair cells and their calyx afferents demonstrate multiple modes of transmission at these specialized endings
Authors: Contini Donatella , Holstein Gay R. , Art Jonathan J. 
Published: March 2022

AbstractIn the vestibular periphery, transmission via conventional synaptic boutons is supplemented by postsynaptic calyceal endings surrounding Type I hair cells. This review focusses on the multiple modes of communication between these receptors and their enveloping calyces as revealed by simultaneous dual-electrode recordings. Classic orthodromic transmission is accompanied by two forms of bidirectional communication enabled by the extensive cleft between the Type I hair cell and its calyx. The slowest cellular communication low-pass filters the transduction current with a time constant 10 — 100 milliseconds: potassium ions accumulate in the synaptic cleft, depolarizing both the hair cell and afferent to potentials greater than necessary for rapid vesicle fusion in the receptor and potentially triggering action potentials in the afferent. On the millisecond timescale, conventional glutamatergic quantal transmission occurs when hair cells are depolarized to potentials sufficient for calcium influx and vesicle fusion. Depolarization also permits a third form of transmission which occurs over tens of microseconds, resulting from the large voltage- and ion-sensitive cleft-facing conductances in both the hair cell and the calyx that are open at their resting potentials. Current flowing out of either the hair cell or the afferent divides into the fraction flowing across the cleft into its cellular partner, and the remainder flowing out of the cleft and into the surrounding fluid compartment. These findings suggest multiple biophysical bases for the extensive repertoire of response dynamics seen in the population of primary vestibular afferent fibers. The results further suggest that evolutionary pressures drive selection for the calyx afferent.
Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality
Authors: Wattrus Samuel J. , Smith Mackenzie L. , Rodrigues Cecilia Pessoa , Hagedorn Elliott J. , Budnik Bogdan , Zon Leonard I. 
Published: March 2022

AbstractTissue-specific stem cells persist for a lifetime and can differentiate to maintain homeostasis or transform to initiate cancer. Despite their importance, there are no described quality assurance mechanisms for newly formed stem cells. We observed intimate and specific interactions between macrophages and nascent blood stem cells in zebrafish embryos. Macrophage interactions led to two outcomes — removal of cytoplasmic material and stem cell division, or complete engulfment and stem cell death. Stressed stem cells were marked by surface Calreticulin, which stimulated macrophage interactions. Using cellular barcoding, we found that calreticulin knock-down or embryonic macrophage depletion reduced the number of stem cell clones that established adult hematopoiesis. Our work supports a model in which embryonic macrophages determine hematopoietic clonality by monitoring stem cell quality.
Cab-sharing services and transformation expectations of consumers: the moderating role of materialism
Authors: Shaikh Ateeque , Mukerjee Kaushik , Banerjee Shubhomoy 
Published: March 2022

PurposeThe study examines the role of attitude, perceived relative advantage and perceived risk on intention to participate in the sharing economy–based cab services in India. Further, it investigates the impact of intention to participate in the sharing economy on transformation expectations of consumers. Finally, the study tests the moderating role of materialism in the relationship between intention to participate in the sharing economy and transformation expectations of consumers.Design/methodology/approachThis study used cross-sectional survey research design to collect data from 408 respondents through online questionnaire in India, an emerging market. The study analysed the data using structural equation modelling technique using IBM AMOS software.FindingsThe findings of the study suggest that perceived relative advantage and attitude influences the intention to participate in the sharing economy. Intention to participate in the sharing economy positively influences transformation expectations. Materialism moderates the relationship between intention to participate and transformation expectations of consumers.Research limitations/implicationsIn a departure from previous studies, this study establishes that perceived risk may not be an important factor driving the intention to participate in the sharing economy. Further, it is among the first studies to establish the role of intention to participate in the sharing economy as a possible driver of transformation expectations.Practical implicationsThe importance of transformation expectations can be communicated as an outcome to encourage participation in the sharing economy. Managers can highlight the relative advantages to promote participation in the sharing economy.Originality/valueThis study is probably the first attempt to understand the transformation expectations of consumers in the sharing economy. Further, the study tests the moderating role of materialism in the relationship between intention to participate and transformation expectation of consumers.
Transformational leadership and service performance for civil servants of public organizations in China: a two-path mediating role of trust
Authors: Fan Lisan , Feng Cailing , Robin Mulyadi , Huang Xiaoyu 
Published: March 2022

Purpose
Transformational leadership and service performance of civil servants greatly affect the government’s administrative effectiveness. However, there are few studies on the influence mechanism of transformational leadership on service performance in the context of public organizations. Based on the social exchange theory, this study aims to construct and examine the dual path mediating process of affective trust and cognitive trust for the effects of transformational leadership on service performance.

Design/methodology/approach
Drawing from 268 supervisor–subordinate dyads civil servants at the municipal level in China across three waves.

Findings
Both affective trust and cognitive trust partly mediated the relationships between transformational leadership and service performance, which supported the underlying theoretical mechanism of social exchange theory and transformational leadership theory in explaining the dual relationship between leaders and subordinates. This study innovatively and empirically examined the effects of transformational leadership on service performance through dual trust in civil servants in China, thus bridging the gap in this knowledge.

Originality/value
This study innovatively and empirically examined the effects of transformational leadership on service performance through dual trust in civil servants in China, thus bridging the gap in this knowledge.
Vedanta Ltd. – attempts to go private
Authors: Das Arindam , Guha Sumantra 
Published: March 2022

Learning outcomes
On completion of analysis of this case, students would be able to: appreciate the context of a typical delisting decision of a public company that is part of a large business group; analyze the complex nature of the relationships among the promoter shareholders, minority shareholders, government-controlled financial institutions, independent directors and executive directors in such a situation of transfer of value; and develop the best possible course of action for the promoters, independent directors and public shareholders, keeping into consideration the principles of corporate governance and the objective of shareholders’ wealth maximization.

Case overview/synopsis
The case presents an opportunity to examine the corporate restructuring and governance issues associated with the delisting attempt of India-based mining company Vedanta Ltd., by its London-based parent company, Vedanta Resources. The case focuses on the conflict of interests between the promoters of a business group and the public shareholders of a subsidiary, and the pivotal roles independent directors and proxy advisory firms play in supporting the public shareholders.

Complexity academic level
The case can be discussed in a graduate-level corporate strategy course that deals with restructuring and governance issues in companies, especially large group companies. It can also be discussed in a course of corporate governance where students have the opportunity to understand the potential conflict between promoters and other shareholders, and the moderating roles the independent directors and institutions may play in resolving such conflicts.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 11: Strategy.
Influence of tax dodging on tax justice in developing countries: some theory and evidence from Sub-Saharan Africa
Authors: Otusanya Olatunde Julius , Liu Jia , Lauwo Sarah George 
Published: March 2022

Purpose
The mobilising domestic resources, in particular, taxation, is key to unlocking the resources required for public investment in infrastructure, growth and sustainable finance. This study aims to share the perception that the tax arrangements of states and the transnational corporations (TNCs) of developed states have a critical effect on the development prospects of the less powerful states in developing countries.

Design/methodology/approach
This paper locates the role of TNCs tax practice within the broader dynamics of globalisation and the pursuit of profits, to argue that the drive of TNCs for higher profits can enrich our understanding of why some TNCs engage in tax dodging. This paper used publicly available evidence to shed light on the role played by TNCs in tax dodging practices in developing countries.

Findings
The evidence shows that tax havens and offshore financial centres, shaped by globalisation, are major structures facilitating the sophisticated tax schemes of highly mobile TNCs. This paper further shows that the corrosive effect of low-tax jurisdictions (“tax havens”) continues to represent a major obstacle to a regulation of global economic relations, which is required for maintaining sustainable social and economic development of poorer states.

Research limitations/implications
This paper used publicly available evidence to illuminate the role played by TNCs in tax dodging practices in Sub-Saharan Africa.

Practical implications
This paper, therefore, advocates a radical reform that could minimise the attendant problems created by the activities of TNCs and the enabling structures that facilitate these practices.

Social implications
Tax dodging has played a major role in causing serious damage to the economic and social landscape in developing countries. This in turn, has undermined social welfare and also investment in the public services, thereby eroding the quality of life and producing a decline in average life expectancy.

Originality/value
This paper is a general review of literature and evidence on contemporary developmental issues.
Evaluating the moderating effect of in-vehicle warning information on mental workload and collision avoidance performance
Authors: Chai Chen , Zhou Ziyao , Yin Weiru , Hurwitz David S. , Zhang Siyang 
Published: March 2022

Purpose
The presentation of in-vehicle warnings information at risky driving scenarios is aimed to improve the collision avoidance ability of drivers. Existing studies have found that driver’s collision avoidance performance is affected by both warning information and driver’s workload. However, whether moderation and mediation effects exist among warning information, driver’s cognition, behavior and risky avoidance performance is unclear.

Design/methodology/approach
This purpose of this study is to examine whether the warning information type modifies the relationship between the forward collision risk and collision avoidance behavior. A driving simulator experiment was conducted with waring and command information.

Findings
Results of 30 participants indicated that command information improves collision avoidance behavior more than notification warning under the forward collision risky driving scenario. The primary reason for this is that collision avoidance behavior can be negatively affected by the forward collision risk. At the same time, command information can weaken this negative effect. Moreover, improved collision avoidance behavior can be achieved through increasing drivers’ mental workload.

Practical implications
The proposed model provides a comprehensive understanding of the factors influencing collision avoidance behavior, thus contributing to improved in-vehicle information system design.

Originality/value
The significant moderation effects evoke the fact that information types and mental workloads are critical in improving drivers’ collision avoidance ability. Through further calibration with larger sample size, the proposed structural model can be used to predict the effect of in-vehicle warnings in different risky driving scenarios.
Effect of ring and rotor spun yarns on denim fabric properties
Authors: Hailemariam Abenezer Fikre , Muhammed Nuredin 
Published: March 2022

Purpose
The purpose of this study is to investigate the mechanical properties of denim fabrics constructed from ring-spun and open-end rotor spun yarns.

Design/methodology/approach
Yarns of 10s Ne count using cotton fibers were spun using the ring and open-end rotor spinning technologies. The yarns were used to produce a denim fabric on an air-jet loom with a 3/1 twill weave structure. Mechanical tests – tensile strength, tear strength, abrasion resistance and pilling resistance – of denim fabrics were evaluated. The test results were analyzed using analysis of variance with the help of Software Package for Social Sciences.

Findings
Denim fabrics made by using ring-spun yarns exhibited better tensile and tear strength properties than denim fabrics made by using open-end rotor spun yarns. On the contrary, denim produced using open-end rotor yarns have better abrasion resistance, pilling resistance and air permeability than those produced using ring-spun yarns.

Originality/value
Both spinning techniques have a significant influence on the properties of denim fabrics. Whenever better tensile and tear strength is required, it is better to use ring-spun yarns, while if the requirement is better abrasion resistance and pilling resistance with high air permeability, then open-end rotor spun yarns shall be used.
The impact of a blockchain platform on trust in established relationships: a case study of wine supply chains
Authors: Brookbanks Mike , Parry Glenn 
Published: March 2022

Purpose
This paper examines the impact of a blockchain platform on the role and importance of trust in established buyer-supplier relationships.

Design/methodology/approach
A literature review provides insight into trust development in supply chains. Research uses a case study of two wine supply chains: the producers, importers, logistics companies and UK Government agencies. Semi-structured interviews determine how trust and trustworthiness develop in buyer-supplier relationships and the impact of a blockchain-based technology proof of concept on supply chain trust.

Findings
A blockchain-based platform introduces common trusted data, reducing data duplication and improving supply chain visibility. The platform supports trust building between parties but does not replace the requirements for organisations to establish a position of trust. Contrary to literature claims for blockchain trustless disintermediation, new intermediaries are introduced who need to be trusted.

Research limitations/implications
The case study presents challenges specific to UK customs borders, and research needs to be repeated in different contexts to establish if findings are generalisable.

Practical implications
A blockchain-based platform can improve supply chain efficiency and trust development but does not remove the need for trust and trust-building processes. Blockchain platform providers need to build a position of trust with all participants.

Originality/value
Case study research shows how blockchain facilitates but does not remove trust, trustworthiness and trust relationships in established supply chains. The reduction in information asymmetry and improved supply chain visibility provided by blockchain does not change the importance of trust in established buyer-supplier relationships or the trust-based policy of the UK Government at the customs border.
Lean in industry 4.0 is accelerating manufacturing excellence – A DEMATEL analysis
Authors: Ojha Ravindra 
Published: March 2022

PurposeThe purpose of this research paper is to study the digital accelerators in conjunction with lean manufacturing enablers in the technology driven Industry 4.0 (I4.0) and understand their interrelationship dynamics with a goal to accelerate the pace of manufacturing excellence.Design/methodology/approachLiterature review coupled with the focus group approach facilitated to cull the key accelerating enablers to lean in I4.0. Thereafter, application of the multi criteria decision making methodology–DEMATEL (Decision Making Trial and Evaluation Laboratory) was carried out for analysis.FindingsA total of 18 factors from the integration of lean in I4.0 were identified from the focus group approach. The analysis from DEMATEL approach reflected that big data analytics and technology driven talent were the two most important factors in the manufacturing excellence journey. Leadership standard work and continuous improvement culture were the two key cause category factors, while, just in time the critical effect category factor.Practical implicationsAnalysis from DEMATEL approach has provided useful insights to industry leaders with the details of the degree of importance and type of influencing factors. It has given them direction in areas of investment to face the challenges of smart factories of tomorrow for sustainability.Originality/valueApplication of DEMATEL approach for analyzing the dynamics of the 18 factors in the integrated lean systems in I4.0 for manufacturing excellence.