bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel hybrid framework based on the ANFIS, discrete wavelet transform, and optimization algorithm for the estimation of water quality parameters
Authors: Kadkhodazadeh Mojtaba , Farzin Saeed 
Published: July 2022

Abstract
Improving the performance of machine learning (ML) algorithms is essential for accurately estimating water quality parameters (WQPs). For the first time, a novel hybrid framework, namely the adaptive neural fuzzy inference system–discrete wavelet transform–gradient-based optimization (ANFIS–DWT–GBO), for estimation of electrical conductivity (EC) and total dissolved solids (TDS), is used. Before estimating WQPs, the performance of the ANFIS–DWT–GBO is proven by several benchmark data sets. In addition, three benchmark algorithms, including ANFIS, ANFIS–DWT, and ANFIS–GBO, are used to demonstrate the strength of the novel framework. The principal component analysis (PCA) method determines the best input combination in EC and TDS estimation. The consequences show that the ANFIS–DWT–GBO produces very successful and competitive results in benchmark data sets modeling and WQPs estimation compared to other algorithms. This result is due to the simultaneous use of DWT and optimization algorithm in the proposed framework. DWT can process WQP data before applying it to the algorithms. The GBO is utilized to optimize the hyperfine parameters in the ANFIS. The results show that the highest accuracy of estimating EC and TDS is in Mollasani and Gotvand stations, respectively. The correlation coefficient (R) value in the Mollasani station is 0.99, and in the Gotvand station it is 0.98.
Endocrine-disruptor endpoints in the ovary and thyroid of adult female rats exposed to realistic doses of di-(2-ethylhexyl) phthalate
Authors: Jebara Amel , Beltifa Asma , Di Bella Guissepa , Mabrouk Lotfi , Ben Mansour Hedi 
Published: July 2022

Abstract
Di-(2-ethylhexyl) phthalate (DEHP) is the world's most widely used polyvinyl chloride (PVC) plasticizer and is used in virtually every category of flexible PVC. In fact, DEHP is extensively used in food cosmetics and medical packaging. It has become a serious problem in recent years. DEHP can be absorbed into the human body through the air, food, water, and skin. The current study involved intraperitoneal injection of DEHP dissolved in corn oil once daily for 21 consecutive days to investigate the effects of DEHP on the thyroid and the reproductive system in female rats. Results show that ovarian hormones (progesterone and estrogen) decreased significantly in the rats treated with DEHP compared to control. This result is supported by the alteration of folliculogenesis, the decrease of the follicles viability, and the apoptosis of the granulosa cells observed on histological sections of ovary and thyroid in female rats exposed to low doses of DEHP. Histopathological study revealed that DEHP could damage thyroid tissue and disrupt these functions. We also observed cellular damage, particularly in the liver cells, and a significant increase in biochemical parameters such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) compared to the control group.
Simulation-optimization model for groundwater replenishment from the river: a case study in the Hutuo River alluvial fan, China
Authors: Zhang Pengwei , Hao Qichen , Fei Yuhong , Li Yasong , Zhu Yuchen , Li Jianfeng 
Published: July 2022

AbstractEcological water replenishment (EWR) has been widely implemented for the restoration of the groundwater resources in the North China Plain since 2018, and the Hutuo River is one of the typical rivers. Recovering the groundwater storage capacity while ensuring the safety of the existing land use is essential for groundwater management. To simulate the groundwater response to different recharge schemes, and to determine the optimal recharge strategy that has the maximum amount of water recharged into the aquifers under specific constraints, an optimization framework, which integrates a 3D transient groundwater flow model with a genetic algorithm (GA), was realized in a Python programming environment in this study. The optimization results show that the optimal amount of water recharged into aquifers is 5.36 × 109 m3 from January 2020 to December 2029, and the upper river reaches are the main recharge area, accounting for 67.58% of the total. Compared with constant recharge, optimal results indicate that the total amount of water recharged into aquifers will increase without exceeding the upper limits of the groundwater levels. However, as groundwater exploitation reduces (18.44%), the river's optimal amount of water recharged also decreases (17.23%). Therefore, the developed model can identify the optimal groundwater recharge strategy and eventually facilitate decision-making in the case of EWR.
Enhanced electro-Fenton degradation of tetracycline in aqueous solution using a self-supported BiOCl/CF cathode
Authors: Kuai Jiangshan , Geng Xiusen , Ren Xiaohua , Guo Weilin 
Published: July 2022

Abstract
The cathode material is critical to the yield of hydrogen peroxide (H2O2) and electro-Fenton (EF) performance. In this work, bismuth oxychloride (BiOCl) as one of the representatives of ternary oxides was grown in situ on carbon felt (CF) through a simple solvothermal method and employed directly as a self-standing cathode for the EF degradation of the target contaminant tetracycline (TC). TC can be almost completely degraded, up to 95% in 90 min under the heterogeneous EF process. The characterizations demonstrated that the BiOCl/CF electrode exhibited excellent conductivity due to CF as the supporting carbon material with a 3D network structure; meanwhile, this hybrid electrode also possessed abundant active sites attributed to the decorated BiOCl having rich oxygen defects. Finally, the rational reaction mechanism of TC was also elucidated by the X-ray photoelectron spectroscopy (XPS) spectrum, free radical quenching experiments and electron paramagnetic resonance (EPR) spectra, in which hydroxyl radicals () were considered as the dominant active oxidants and BiOCl had a synergistic effect on in-situ generation and decomposition of H2O2.
HIGHLIGHTS A BiOCl/CF composite electrode was prepared via a simple solvothermal method. Oxygen defects enriched BiOCl and bare carbon felt cathode had a synergistic effect on H2O2 production. Lattice oxygen reduced Bi3+ to Bi0, facilitating the formation of by in situ reaction of hydrogen peroxide with Bi0. was the main radical in catalytic degradation of tetracycline.
Regulatory Mechanism of lncRNA CTBP1-AS2 in Nasopharyngeal
Carcinoma Cell Proliferation and Apoptosis via the miR-140-5p/BMP2 Axis
Authors: Huang Bo , Li Yiliang , Deng Zhuoxia , Lan Guiping , Si Yongfeng , Zhou Qiao 
Published: July 2022

Objective:
Nasopharyngeal carcinoma (NPC) is a squamous cell carcinoma. LncRNA CTBP1-AS2 (CTBP1-AS2) has effects on tumor cell growth. This study explored the mechanism of CTBP1-AS2 on NPC cells.

Methods:
CTBP1-AS2 expressions in immortalized nasopharyngeal epithelial (NP69) and 6 human NPC cells were detected by RT-qPCR, and SUNE-1/CNE-1 cells with relative high/low expressions were selected. Cell proliferation and apoptosis were detected by CCK-8, colony formation assays, and flow cytometry. The binding sites between CTBP1-AS2 and miR-140-5p, and miR-140-5p and BMP2 were predicted and the binding relationships were verified by dual-luciferase assay. BMP2 level was detected by Western blot. miR-140-5p was silenced or BMP2 was overexpressed in SUNE-1 cells with si-CTBP1-AS2 to study the effects of miR-140-5p and BMP2 on CTBP1-AS2 silencing-inhibited malignant behaviors.

Results and Discussion:
CTBP1-AS2 was upregulated in NPC cells. CTBP1-AS2 silencing suppressed NPC cell proliferation and promoted apoptosis. CTBP1-AS2 silencing in SUNE-1 cells raised miR-140-5p expression and repressed BMP2 level. CTBP1-AS2 overexpression in CNE-1 cells suppressed miR-140-5p expression and elevated BMP2 levels. In mechanism, miR-140-5p overexpression decreased BMP2 levels, reduced the NPC cell proliferation and promoted apoptosis. miR-140-5p knockdown or BMP2 overexpression enhanced NPC cell proliferation and inhibited apoptosis, thus restoring NPC cell malignant behaviors inhibited by silencing CTBP1-AS2.

Conclusion:
CTBP1-AS2 decreased miR-140-5p-induced BMP2 inhibition via functioning as a ceRNA of miR-140-5p and promoted BMP2 expression, thereby promoting NPC cell proliferation and suppressing apoptosis.
Oxytocin: An Old Hormone, a Novel Psychotropic Drug and its Possible Use in Treating Psychiatric Disorders
Authors: Marazziti Donatella , Diep Phuoc-Tan , Carter Sue , Carbone Manuel Glauco 
Published: July 2022

Background:
Oxytocin is a nonapeptide synthesized in the paraventricular and supraoptic
nuclei of the hypothalamus. Historically, this molecule has been involved as a key factor in
the formation of infant attachment, maternal behavior and pair bonding and, more generally, in linking
social signals with cognition, behaviors and reward. In the last decades, the whole oxytocin system
has gained a growing interest as it was proposed to be implicated in etiopathogenesis of several
neurodevelopmental and neuropsychiatric disorders.

Methods:
With the main goal of an in-depth understanding of the oxytocin role in the regulation
of different functions and complex behaviors as well as its intriguing implications in different
neuropsychiatric disorders, we performed a critical review of the current state of the art.
We carried out this work through the PubMed database up to June 2021 with the search terms:
1) “oxytocin and neuropsychiatric disorders”; 2) “oxytocin and neurodevelopmental disorders”;
3) “oxytocin and anorexia”; 4) “oxytocin and eating disorders”; 5) “oxytocin and obsessive-
compulsive disorder”; 6) “oxytocin and schizophrenia”; 7) “oxytocin and depression”; 8)
“oxytocin and bipolar disorder”; 9) “oxytocin and psychosis”; 10) “oxytocin and anxiety”; 11)
“oxytocin and personality disorder”; 12) “oxytocin and PTSD”.

Results:
Biological, genetic, and epigenetic studies highlighted quality and quantity modifications
in the expression of oxytocin peptide or in oxytocin receptor isoforms. These alterations
would seem to be correlated with a higher risk of presenting several neuropsychiatric disorders
belonging to different psychopathological spectra. Collaterally, the exogenous oxytocin administration
has shown to ameliorate many neuropsychiatric clinical conditions.

Conclusion:
Finally, we briefly analyzed the potential pharmacological use of oxytocin in a
patient with severe symptomatic SARS-CoV-2 infection due to its anti-inflammatory, antioxidative
and immunoregulatory properties.
Recent Advances in Immunotherapy for the Treatment of Malignant Melanoma
Authors: Tuli Hardeep Singh , Sak Katrin , Iqubal Ashif , Choudhary Renuka , Adhikary Shubham , Kaur Ginpreet , Kumar Pawan , Garg Vivek Kumar 
Published: July 2022

Abstract:
Malignant melanoma is one of the most worrisome malignancies due to its fast dissemination and
early formation of metastases in multiple sites throughout the body. Despite the intensive efforts made in the
last decades, the use of standard therapeutic strategies, including chemotherapy and radiotherapy, has not led to
a substantial improvement in clinical outcomes, mainly because of the intrinsic resistance of melanomas to these
treatment modalities. Therefore, in recent years, numerous studies have focused on the possibility of boosting
the clinical responses of melanoma patients by using novel immunotherapeutic agents. In this review article,
a comprehensive survey is presented about the approved immunotherapeutic drugs and their action mechanisms,
besides describing the agents that are currently still in clinical trials. Moreover, the combination of immunotherapeutic
drugs with conventional approaches, i.e., radiotherapy, chemotherapy, and targeted therapy, is
another focal point of this review, providing valuable input for further elaboration of the best treatment regimens
to prolong survival and improve the quality of life of melanoma patients.
Current Drug Targeting using siRNA-based Nano Therapeutics for Pulmonary
Diseases
Authors: Krishnaswami Venkateshwaran , Kandasamy Ruckmani 
Published: July 2022

Abstract:
The importance of siRNA in nano drug delivery systems to target important pulmonary disorders,
such as chronic obstructive pulmonary disease (COPD), asthma, lung cancer, and others, is reviewed in this
perspective. The great majority of lung illnesses are caused by protein misfolding. As a result, siRNA-based
therapies are increasingly being used to target the gene. Given the difficulties of delivering bare siRNA, siRNA
protection may ensure its efficacy in gene therapy. These issues could be solved with a nano-based siRNA
delivery systems. In this context, a siRNA-based nanocarrier for major pulmonary disorders has been explored.
TIPE2 Inhibits Migration and Promotes Apoptosis as a Tumor Suppressor
in Hypopharyngeal Carcinoma
Authors: Wang Juan , Pan Xinliang , Yang Xiaoqi , Zhang Minfa , Su Tongdong , Tang Shuangmei , Wang Yin , Liu Heng , Wang Pin 
Published: July 2022

Background:
Hypopharyngeal squamous cell carcinoma (HSCC) is a common malignant
cancer characterized by high metastasis and infiltration. The development of new approaches for the
early diagnosis and identification of new therapeutic targets is essential. TIPE2 is well known as a tumor
suppressor and related to a favorable prognosis of HSCC. However, its underlying mechanism
remains unclear.

Methods and Materials:
TIPE2 expression was determined by immunohistochemistry and RT-qPCR.
A TIPE2 overexpression stable cell line was generated by lentivirus infection. TIPE2 and other related
protein levels were detected by western blotting. The cell cycle and apoptosis were performed by flow
cytometric analysis. Cell proliferation was measured with a Cell Counting Kit-8 (CCK-8) assay, and
the activity of caspase-3 and caspase-7 was assessed by Caspase-Glo® 3/7 Assay. All data were analyzed
with SPSS 25 and GraphPad Prism 8.0.

Results:
TIPE2 expression was significantly down-regulated in HSCC. Low TIPE2 expression may be
associated with poor prognosis in HSCC. TIPE2 overexpression markedly inhibited tumor cell migration.
Moreover, TIPE2 decreased cell proliferation but promoted apoptosis. TIPE2 suppressed tumor
growth by activating Epithelial-Mesenchymal Transition (EMT) and the extrinsic apoptosis pathway.

Conclusion:
TIPE2 inhibited tumor progression by suppressing cell migration but promoting apoptosis.
TIPE2 can be a new therapeutic target in HSCC.
Impact of Azo Dyes and Ibuprofen on the Proteome of Serratia nematodiphila
sp. MB307
Authors: Yasmin Azra , Basharat Zarrin , Moon Kyung-Mee , Foster Leonard J. 
Published: July 2022

Background:
Micropollutants comprise organic/mineral substances that cause an undesirable
impact on the environment, by affecting life at all scales. In this study, we explored the changes
they impart on the global proteome of a soil bacterium Serratia nematodiphila MB307, for two classes
of pollutants, i.e., Azo dyes (Methyl orange, Congo red) and a pharmaceutical (Ibuprofen).

Methods:
The 100 μg pollutant supplemented alteration of pure S. nematodiphila MB307 culture after
24 hours of incubation at 37 oC and its control was analyzed using a differential proteomics approach.
MaxQuant software with the Perseus package was used for data analysis purposes.

Results:
Prominently, ribosomal proteins and chaperones were up or downregulated in the whole cell
and membranous fraction.

Conclusion:
This illustrates dynamic protein production adaptation of bacteria, to cope with stress and
cell growth/division trade-off for survival. A collective pattern of survival under stress or pollution
resistance could not be decrypted for all classes of pollutants, portraying dissimilar mechanisms of
coping with differently structured pollutant moieties.
OMICs Technologies for Natural Compounds-based Drug Development
Authors: Aborode Abdullahi Tunde , Awuah Wireko Andrew , Mikhailova Tatiana , Abdul- Rahman Toufik , Pavlock Samantha , Kundu Mrinmoy , Yarlagadda Rohan , Pustake Manas , Correia Inês Filipa da Silva , Mehmood Qasim , Shah Parth , Mehta Aashna , Ahmad Shahzaib , Asekun Abiola , Nansubuga Esther Patience , Amaka Shekinah Obinna , Shkodina Anastasiia Dmytrivna , Alexiou Athanasios 
Published: July 2022

Abstract:
Compounds isolated from natural sources have been used for medicinal purposes for
many centuries. Some metabolites of plants and microorganisms possess properties that would
make them effective treatments against bacterial infection, inflammation, cancer, and an array of
other medical conditions. In addition, natural compounds offer therapeutic approaches with lower
toxicity compared to most synthetic analogues. However, it is challenging to identify and isolate potential
drug candidates without specific information about structural specificity and limited
knowledge of any specific physiological pathways in which they are involved. To solve this problem
and find a way to efficiently utilize natural sources for the screening of compounds candidates,
technologies, such as next-generation sequencing, bioinformatics techniques, and molecular analysis
systems, should be adapted for screening many chemical compounds. Molecular techniques capable
of performing analysis of large datasets, such as whole-genome sequencing and cellular protein
expression profile, have become essential tools in drug discovery. OMICs, as genomics, proteomics,
and metabolomics, are often used in targeted drug discovery, isolation, and characterization.
This review summarizes technologies that are effective in natural source drug discovery and aid in a
more precisely targeted pharmaceutical approach, including RNA interference or CRISPR technology.
We strongly suggest that a multidisciplinary effort utilizing novel molecular tools to identify
and isolate active compounds applicable for future drug discovery and production must be enhanced
with all the available computational tools.
Decomposition Kinetics of 1,1-Dihydroperoxycyclohexane in Some Organic
Solvents
Authors: Grabovskii Stanislav A. , Andriyashina Nadezhda M. , Safiullin Rustam L. 
Published: July 2022

Background:
1,1-Bishydroperoxides are used as oxidizing agents, radical initiators, in addition,
studies of biological activity show the promise of this area of research.

Objective:
There are currently no kinetic data on the thermal stability of these peroxides in various solvents.
Therefore, we investigated the thermal stability of 1,1-dihydroperoxycyclohexane as the most
widely used at present.

Methods:
The rate constants and activation parameters were measured at the temperature range of 100-
170oC in the presence of 2-naphthol, which inhibits the induced decomposition of the peroxide by free
radicals. Peroxide concentration was determined by iodometric method.

Results:
The rate constants of the reaction with the solvents and of the monomolecular decomposition
of the peroxide were determined.

Conclusion:
The use of DHPC as a radical initiator requires taking into account its reactions with the
solvent and/or monomer, in addition to its monomolecular decomposition with the formation of free
radicals.
Do Quercetin and Vitamin E Properties Preclude Doxorubicin-induced
Stress and Inflammation in Reproductive Tissues?
Authors: Zal Fatemeh , Samare-Najaf Mohammad , Jamali Navid , Vakili Sina , Khodabandeh Zahra 
Published: July 2022

Background:
In recent decades, the exposure to doxorubicin (DOX) has elevated due to the increment in the incidence of cancer, especially among the young population, which, despite the desired restorative impacts, threatened the quality of life of survivors, particularly concerning their reproductive ability.

Objectives:
Although previous studies have shown the effectiveness of quercetin (QCT) and vitamin E (Vit.E), two major dietary antioxidants with favorable attributes regarding the female reproductive system, on doxorubicin-induced insulting to the ovary and uterus. The mechanisms involved in responding to stress and inflammation have not been elucidated. Hence, this study sought to evaluate the preventive effects of these two antioxidants on doxorubicin-induced disruption of ovarian and uterine stress and inflammation.

Methods:
The study involved 48 female rats that were equally allocated into 6 groups as control (CON), QCT (20mg/Kg), Vit.E (200mg/Kg), DOX (accumulative 15mg/Kg), DOX+QCT, and DOX+Vit.E. Upon 21 days treatment, the activity of Superoxide Dismutase (SOD), Catalase (CAT), Glutathione-dependent system, Total Antioxidant Capacity (1), Malondialdehyde (MDA), Nitric Oxide (NO), and Tumor Necrosis Factor-alpha (TNF-#945;&in the reproductive tissues and serum were evaluated.

Results:
Findings demonstrated that the levels of CAT, SOD, Glutathione Peroxidase (GPx), and TAC were alleviated by the studied antioxidants in both tissues (p-value<0.05). Furthermore, both supplements revealed ameliorative effects on DOX-induced alterations in NO, MDA (p-value<0.001), and TNF-&#945levels.

Conclusion:
Taking together, the present findings suggested the promising alleviative properties of QCT and Vit.E via modulating stress- and inflammation-responsive mechanisms against DOX-induced female reproductive toxicity.
Developing a Gagne theory-based learning video for thematic subject in elementary school
Authors: Putra I Gede Widiartana , Warpala I Wayan Sukra , Sudatha I Gde Wawan , Utami Ni Putu Rara Stianing 
Published: July 2022

The COVID-19 has shown many significant impacts in life such as in educational field which required students and teachers to conduct a blended learning using creative learning media. It should be able to make the students learning independently. However, that was not an easy thing since the teacher only used a conventional learning media: lecturing and textbook. To solve it, this research aimed to design learning media which focused on Tematic Subjects from Indonesian curicculum of 2013. The development was focused on Science subject only. The development model used ADDIE model and implemented Gagne's 9 steps/events of learning theory. The results of the research found that (1) design of Science learning video with the ADDIE model, (2) Science learning video is valid with resulted: good predicate (80.4%) on expert review of the subject matters, very good predicate (97, 7%) on expert review of the Science learning video media, very good predicate (94.1%) on learning design expert review, very good predicate (93.76%) on individual test, very good predicate (92.61%) on small group test, and very good predicate (90.45%) on field test. Importantly, (3) the effectiveness of development concluded that there was a significant difference between the results of pretest and posttest.