bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatiotemporal variation characteristics of livestock manure nutrient in the soil environment of the Yangtze River Delta from 1980 to 2018
Authors: Zhao Shuyan , Chen Yujiao , Gu Xiaomin , Zheng Mengru , Fan Ziyi , Luo Dan , Luo Kaihong , Liu Bo 
Published: May 2022

AbstractThe pollution problem caused by surplus livestock manure nutrient is becoming more and more serious; thus, analyzing characteristics of the temporal and spatial patterns of livestock manure nutrient and assessing the risks are extremely important. The pollution coefficient method was used to figure out the spatiotemporal variation characteristics of the nitrogen (N) and phosphorus (P) emissions of livestock manure nutrient in soil of the Yangtze River Delta from 1980 to 2018. High-risk areas for livestock manure pollution were determined by matching degree assessment between livestock manure nutrient supply and crop nutrient requirements. Clustering and principal component analysis were applied to select main control factors. The results found that the total discharge and manure N and P loads in animal manure nutrient showed an overall trend of increasing first and then decreasing in the Yangtze River Delta in the soil from 1980 to 2018. The center of manure N and P loads shifted from the central region to the northeastern and southwestern regions. There were four typical patterns for manure N emissions, the main control factors were meat production and primary industry. Meanwhile, the main control factors for the four typical patterns of manure P emissions were meat production and cultivated land area. This research provides a theoretical basis for the sustainable development of the livestock breeding industry and is of significance for promoting a balance between planting and breeding in the Yangtze River Delta.
Microscopic fabric evolution and macroscopic deformation response of gangue solid waste filler considering block shape under different confining pressures
Authors: Chen Liang , Li Junmeng , Zhang Dongsheng , Fan Gangwei , Zhang Wei , Guo Yachao 
Published: May 2022

AbstractThe irregular shape of gangue blocks will affect the coordination structure between blocks in the crushed gangue accumulation body, and then affect the engineering mechanical properties of crushed gangue in the process of load-bearing compression. In this paper, through CT scanning experiment, particle flow numerical simulation experiment, and comprehensive application of image processing, 3D reconstruction, FLAC/PFC3D continuum—discrete coupling technology, the gangue digital 3D model and the numerical model of crushed gangue particle flow under triaxial compression condition considering the real shape of the block were obtained. The microscopic fabric evolution law and macroscopic deformation response characteristics of crushed gangue considering triaxial compression condition and different confining pressures were studied. The results show that: (1) the bearing capacity of crushed gangue materials increases with the increase of confining pressure; (2) the block aggregate in the gangue sample is gradually compacted, and the lateral deformation of the sample is changed from “extruding to the axis” to “bulging to the periphery”; (3) the vertical movement of the block decreases gradually from the top to the bottom of the sample, and there is a “triangle area” of block displacement at the top and bottom of the sample; the larger the confining pressure, the smaller the vertical displacement range at the top of the sample; (4) the process of “instability and failure—optimization and reconstruction” of skeleton force chain structure occurs constantly; as confining pressure increases, the stability of skeleton force chain structure and the bearing capacity of crushed gangue sample increases; (5) under the same strain state, the greater the confining pressure, the higher the fragmentation degree of the sample. This study reveals the internal mechanism of macro deformation of crushed gangue under the triaxial compression from the perspective of the mesoscopic fabric evolution. The research results are of great significance for the selection of crushed gangue in engineering application. In addition, the research results also have a significant impact on promoting the reasonable disposal and resource utilization of gangue solid waste and protecting the ecological environment of mining areas.
KATP channel dependent heart multiome atlas
Authors: Arrell D. Kent , Park Sungjo , Yamada Satsuki , Alekseev Alexey E. , Garmany Armin , Jeon Ryounghoon , Vuckovic Ivan , Lindor Jelena Zlatkovic , Terzic Andre 
Published: May 2022

AbstractPlasmalemmal ATP sensitive potassium (KATP) channels are recognized metabolic sensors, yet their cellular reach is less well understood. Here, transgenic Kir6.2 null hearts devoid of the KATP channel pore underwent multiomics surveillance and systems interrogation versus wildtype counterparts. Despite maintained organ performance, the knockout proteome deviated beyond a discrete loss of constitutive KATP channel subunits. Multidimensional nano-flow liquid chromatography tandem mass spectrometry resolved 111 differentially expressed proteins and their expanded network neighborhood, dominated by metabolic process engagement. Independent multimodal chemometric gas and liquid chromatography mass spectrometry unveiled differential expression of over one quarter of measured metabolites discriminating the Kir6.2 deficient heart metabolome. Supervised class analogy ranking and unsupervised enrichment analysis prioritized nicotinamide adenine dinucleotide (NAD+), affirmed by extensive overrepresentation of NAD+ associated circuitry. The remodeled metabolome and proteome revealed functional convergence and an integrated signature of disease susceptibility. Deciphered cardiac patterns were traceable in the corresponding plasma metabolome, with tissue concordant plasma changes offering surrogate metabolite markers of myocardial latent vulnerability. Thus, Kir6.2 deficit precipitates multiome reorganization, mapping a comprehensive atlas of the KATP channel dependent landscape.
Groove rhythm stimulates prefrontal cortex function in groove enjoyers
Authors: Fukuie Takemune , Suwabe Kazuya , Kawase Satoshi , Shimizu Takeshi , Ochi Genta , Kuwamizu Ryuta , Sakairi Yosuke , Soya Hideaki 
Published: May 2022

AbstractHearing a groove rhythm (GR), which creates the sensation of wanting to move to the music, can also create feelings of pleasure and arousal in people, and it may enhance cognitive performance, as does exercise, by stimulating the prefrontal cortex. Here, we examined the hypothesis that GR enhances executive function (EF) by acting on the left dorsolateral prefrontal cortex (l-DLPFC) while also considering individual differences in psychological responses. Fifty-one participants underwent two conditions: 3 min of listening to GR or a white-noise metronome. Before and after listening, participants performed the Stroop task and were monitored for l-DLPFC activity with functional near-infrared spectroscopy. Our results show that GR enhanced EF and l-DLPFC activity in participants who felt a greater groove sensation and a more feeling clear-headed after listening to GR. Further, these psychological responses predict the impact of GR on l-DLPFC activity and EF, suggesting that GR enhances EF via l-DLPFC activity when the psychological response to GR is enhanced.
Early life circadian rhythm disruption in mice alters brain and behavior in adulthood
Authors: Ameen Rafal W. , Warshawski Allison , Fu Lucia , Antle Michael C. 
Published: May 2022

AbstractHealthy sleep supports robust development of the brain and behavior. Modern society presents a host of challenges that can impair and disrupt critical circadian rhythms that reinforce optimal physiological functioning, including the proper timing and consolidation of sleep. While the acute effects of inadequate sleep and disrupted circadian rhythms are being defined, the adverse developmental consequences of disrupted sleep and circadian rhythms are understudied. Here, we exposed mice to disrupting light–dark cycles from birth until weaning and demonstrate that such exposure has adverse impacts on brain and behavior as adults. Mice that experience early-life circadian disruption exhibit more anxiety-like behavior in the elevated plus maze, poorer spatial memory in the Morris Water Maze, and impaired working memory in a delayed match-to-sample task. Additionally, neuron morphology in the amygdala, hippocampus and prefrontal cortex is adversely impacted. Pyramidal cells in these areas had smaller dendritic fields, and pyramidal cells in the prefrontal cortex and hippocampus also exhibited diminished branching orders. Disrupted mice were also hyperactive as adults, but otherwise exhibited no alteration in adult circadian locomotor rhythms. These results highlight that circadian disruption early in life may have long lasting and far-reaching consequences for the development of behavior and the brain.
Gender differences in frailty transition and its prediction in community-dwelling old adults
Authors: Mielke Nina , Schneider Alice , Huscher Dörte , Ebert Natalie , Schaeffner Elke 
Published: May 2022

AbstractFrailty is very common in old age and often associated with adverse events. Transitioning between frailty states is possible in both directions (improvement and worsening) offering targets for interventions. Frailty is more prevalent in women, but little is known about the impact of gender on frailty transition. The aim of this study is to identify gender differences for frailty transition in older adults and to develop gender-stratified prognostic prediction models for frailty transition. We performed a longitudinal analyses of the Berlin Initiative (cohort) Study with a frailty follow-up of 2.1 years. Description of frailty transition using the frailty phenotype and development of prognostic prediction models using multivariable logistic regressions for transition (improvement or worsening) stratified by gender following the TRIPOD statement were performed. In total, the study population consisted of 1158 community-dwelling adults with a mean age of 84.4 years and of whom 55% were women. Out of 1158 participants 225 (19%) were robust, 532 (46%) prefrail and 401 (35%) frail. After 2.1 (IQR 2.0–2.3) years, half of the participants had transitioned between frailty states. Men worsened more often and those who were already frail died more often than women. Gender-stratified prediction models for frailty transition demonstrated that some predictors (age, self-rated health, cognitive impairment, baseline frailty status) were included in all models. While stroke, diabetes mellitus, smoking and glomerular filtration rate were unique predictors in the models for females, osteoarthritis, hospitalization and education were predictors in the models for males. There are gender differences in frailty transition rates, patterns and prediction. This supports the importance of considering gender when addressing frailty and targeting interventions in old age.
Linear magnetoelastic coupling and magnetic phase diagrams of the buckled-kagomé antiferromagnet $$\hbox {Cu}_3\hbox {Bi}{(\hbox {SeO}_3)_2}\hbox {O}_2\hbox {Cl}$$
Authors: Spachmann S. , Berdonosov P. , Markina M. , Vasiliev A. , Klingeler R. 
Published: May 2022

AbstractSingle crystals of Cu$$_3$$

3

Bi(SeO$$_3$$

3

)$$_2$$

2

O$$_2$$

2

Cl were investigated using high-resolution capacitance dilatometry in magnetic fields up to 15 T. Pronounced magnetoelastic coupling is found upon evolution of long-range antiferromagnetic order at $$T_{\mathrm {N}}$$

T
N

$$= 26.4(3)$$

=
26.4
(
3
)

K. Grüneisen analysis reveals moderate effects of uniaxial pressure on $$T_{\mathrm {N}}$$

T
N

, of 1.8(4) K/GPa, $$-0.62(15)$$

-
0.62
(
15
)

K/GPa and 0.33(10) K/GPa for $$p \parallel a$$

p
‖
a

, b, and c, respectively. Below 22 K Grüneisen scaling fails which implies the presence of competing interactions. The structural phase transition at $$T_{\mathrm {S}}$$

T
S

$$= 120.7(5)$$

=
120.7
(
5
)

K is much more sensitive to uniaxial pressure than $$T_{\mathrm {N}}$$

T
N

, with strong effects of up to 27(3) K/GPa ($$p \parallel c$$

p
‖
c

). Magnetostriction and magnetization measurements reveal a linear magnetoelastic coupling for $$B\parallel c$$

B
‖
c

below $$T_{\mathrm {N}}$$

T
N

, as well as a mixed phase behavior above the tricritical point around 0.4 T. An analysis of the critical behavior in zero-field points to three-dimensional (3D) Ising-like magnetic ordering. In addition, the magnetic phase diagrams for fields along the main crystalline axes are reported.
Beyond comorbidities, sex and age have no effect on COVID-19 health care demand
Authors: Mendes Jorge M. , Baptista Helena , Oliveira André , Jardim Bruno , de Castro Neto Miguel 
Published: May 2022

AbstractThis paper explores the associations between sex, age and hospital health care pressure in the context of the COVID-19 pandemic in Portuguese mainland municipalities. To represent the impact of sex and age, we calculated COVID-19 standardised incidence ratios (SIR) in Portuguese mainland municipalities over fourteen months daily, especially focusing on the Porto metropolitan area. A daily novel indicator was devised for hospital health care pressure, consisting of an approximation to the ratio of hospitalisations per available hospital medical doctor (HPI). In addition, 14-day incidence rates were also calculated daily (DIR14), both as an approach and an alternative to the current national pandemic surveillance indicator (which is not calculated with such regularity). Daily maps were first visualised to evaluate spatial patterns. Pearson's correlation coefficients were then calculated between each proposed surveillance indicator (SIR and DIR14) and the HPI. Our results suggest that hospital pressure is not strongly associated with SIR (r = 0.34, p value = 0.08). However, DIR14 bears a stronger correlation with hospital pressure (r = 0.84, p value < 0.001). By establishing the importance of tackling sex and age through the inclusion of these factors explicitly in an epidemiological monitoring indicator, and assessing its relationship with a hospital pressure indicator, our findings have public policy implications that could improve COVID-19 incidence surveillance in Portugal and elsewhere, contributing to advancing the management of potential pandemics in the near future, with a particular focus on local and regional territorial scales.
Assessment of age, gender, and anxiety on ECG waveform morphology in a large population of domestic dogs
Authors: Murphy Lisa , Nakamura Reid , Gentile-Solomon Jessica , Spake Allison , Szlosek Donald 
Published: May 2022

AbstractCardiovascular diseases are major causes of death in the western world and this incidence increases in the elderly population. With aging, there are physiologic changes to the cardiac structure secondary to adipose tissue deposition, calcification of valve leaflets and changes in the structure of the heart including atrial remodeling. Such changes can make the myocardium more susceptible to stress leading to a higher prevalence of cardiovascular diseases in the aging population. Studies in healthy humans have shown that these structural and molecular changes in the heart are manifested as changes on an electrocardiogram (ECG). Using animal models, similar ECG changes have been found in guinea pigs, rabbits, and mice. No veterinary study has specifically evaluated if comparable aging changes occur in canine species. In this cross-sectional retrospective study, 12,026 ECGs from apparently healthy dogs were obtained and evaluated. Age was observed to have both linear and non-linear associations with multiple ECG variables, including P wave amplitude and duration, R amplitude and QRS duration. This study confirmed that, like humans, there may be ECG changes secondary to normal physiological cardiac aging. Further studies are warranted to confirm and elaborate on these findings as canines may be a useful model for cardiac aging in humans.
Novel passive detection approach reveals low breeding season survival and apparent lactation cost in a critically endangered cave bat
Authors: van Harten Emmi , Lawrence Ruth , Lumsden Lindy F. , Reardon Terry , Prowse Thomas A. A. 
Published: May 2022

AbstractCapture-mark-recapture/resight (CMR) methods are used for survival-rate studies, yet are often limited by small sample sizes. Advances in passive integrated transponder (PIT) technology have enabled passive detection or ‘resight’ of marked individuals using large antennas with greater read-ranges than previously possible. We used passively-detected resight data and CMR models to study survival rates of the southern bent-winged batMiniopterus orianae bassanii, a critically endangered, cave-dwelling bat. Over three years, we used PIT-tagging to monitor 2966 individuals at the species’ largest breeding aggregation, using daily detection data (> 1.6 million detections) to estimate seasonal survival probabilities, structured by age, sex and reproductive status, and parameterise population projection matrices. This has hitherto been impossible using traditional CMR methods due to disturbance risk and low recapture rates. Bats exhibited lowest apparent seasonal survival over summer and autumn, particularly for reproductive females in summer (when lactating) and juveniles in autumn (after weaning), and high survival in winter. Lowest survival rates coincided with severe drought in summer–autumn 2016, suggesting that dry conditions affect population viability. Under all likely demographic assumptions, population projection matrices suggested the population is in deterministic decline, requiring urgent action to reduce extinction risk. Passively-collected resight data can now be used in combination with CMR models to provide extensive, robust information for targeted wildlife population management.
Cytotoxicity of polymers intended for the extrusion-based additive manufacturing of surgical guides
Authors: Burkhardt Felix , Spies Benedikt C. , Wesemann Christian , Schirmeister Carl G. , Licht Erik H. , Beuer Florian , Steinberg Thorsten , Pieralli Stefano 
Published: May 2022

AbstractExtrusion-based printing enables simplified and economic manufacturing of surgical guides for oral implant placement. Therefore, the cytotoxicity of a biocopolyester (BE) and a polypropylene (PP), intended for the fused filament fabrication of surgical guides was evaluated. For comparison, a medically certified resin based on methacrylic esters (ME) was printed by stereolithography (n = 18 each group). Human gingival keratinocytes (HGK) were exposed to eluates of the tested materials and an impedance measurement and a tetrazolium assay (MTT) were performed. Modulations in gene expression were analyzed by quantitative PCR. One-way ANOVA with post-hoc Tukey tests were applied. None of the materials exceeded the threshold for cytotoxicity (< 70% viability in MTT) according to ISO 10993-5:2009. The impedance-based cell indices for PP and BE, reflecting cell proliferation, showed little deviations from the control, while ME caused a reduction of up to 45% after 72 h. PCR analysis after 72 h revealed only marginal modulations caused by BE while PP induced a down-regulation of genes encoding for inflammation and apoptosis (p < 0.05). In contrast, the 72 h ME eluate caused an up-regulation of these genes (p < 0.01). All evaluated materials can be considered biocompatible in vitro for short-term application. However, long-term contact to ME might induce (pro-)apoptotic/(pro-)inflammatory responses in HGK.
Excretion and viability of SARS-CoV-2 in feces and its association with the clinical outcome of COVID-19
Authors: Cerrada-Romero Cristina , Berastegui-Cabrera Judith , Camacho-Martínez Pedro , Goikoetxea-Aguirre Josune , Pérez-Palacios Patricia , Santibáñez Sonia , José Blanco-Vidal María , Valiente Adoración , Alba Jorge , Rodríguez-Álvarez Regino , Pascual Álvaro , Oteo José Antonio , Miguel Cisneros José , Pachón Jerónimo , Casas-Flecha Inmaculada , Cordero Elisa , Pozo Francisco , Sánchez-Céspedes Javier 
Published: May 2022

AbstractThe main objective was to evaluate the viability of the SARS-CoV-2 viral particles excreted in stools. In addition, we aimed to identify clinical factors associated with the detection of SARS-CoV-2 RNA in feces, and to determine if its presence is associated with an unfavorable clinical outcome, defined as intensive care unit (ICU) admission and/or death. A prospective multicenter cohort study of COVID-19 adult patients, with confirmed SARS-CoV-2 infection by RT-PCR assay in nasopharyngeal (NP) swabs admitted to four hospitals in Spain, from March 2020 to February 2021. Sixty-two adult COVID-19 patients had stool samples collected at admission and/or during the follow up, with a total of 79 stool samples. SARS-CoV-2 RNA was detected in stool samples from 27 (43.5%) out of the 62 patients. Replicative virus, measured by the generation of cytopathic effect in cell culture and subsequent RT-PCR confirmation of a decrease in the Ct values, was not found in any of these stool samples. Fecal virus excretion was not associated with the presence of gastrointestinal symptoms, or with differences in the evolution of COVID-19 patients. Our results suggest that SARS-CoV-2 replicative capacity is null or very limited in stool samples, and thus, the fecal–oral transmission of SARS-CoV-2 as an alternative infection route is highly unlikely. In our study, the detection of SARS-CoV-2 RNA in feces at the beginning of the disease is not associated with any clinical factor nor with an unfavorable clinical outcome.
Fluorescence and UV/visible spectroscopic investigation of orange and mango fruit juice quality in case of Adama Town
Authors: Gebishu Muktar , Fikadu Boka , Bekele Bulcha , Tesfaye Jule Leta , N Nagaprasad. , Ramaswamy Krishnaraj 
Published: May 2022

AbstractExtracted Mango and Orange juices were investigated by using spectroscopic techniques such as UV/Visible and Fluorescence. Three portions of samples (fresh juice) were stored at 22 °C for eight days, stored in a water bath and heated at 40 °C, 60 °C, and 80 °C for ten minutes. The highest wavelengths (455 nm) were observed from the UV/Vis results for fresh Mango juices, while 270 nm and 460 nm were observed for stored Mango juices. Furthermore, wavelengths of 320 nm were observed in heat-treated mango juice (40 °C). No absorption peaks were observed at 60 °C and 80 °C due to temperature effects. Absorption peaks of fresh fruit were observed at 330 nm and 390 nm, while 260 nm and 320 nm reflect stored orange juices absorptions peaks. From heat-treated stored (40 °C and 60 °C) samples, 320 nm and 260 nm absorption peaks were observed, respectively. Wavelength observed (454 nm, 540 nm & 700 nm) peaks represent the fresh mango juice spectra, while 460 nm and 700 nm are for stored Mango juices. The peaks observed in the region of 400–500 nm and at 700 nm represent heat-treated mango juices at 40 °C. Heat stored Mango juices (60 °C & 80 °C) have peaks at 700 nm. Peaks observed at 700 nm, 500 nm, and 455 nm reflect fresh orange juice, while 460–500 nm and 700 nm represent the emission spectra of the samples. The stored orange juice peaks at 460–500 nm and at 700 nm, but heated-stored orange juice peaks only at 700 nm. The pH values for orange and mango juices were 3.52–3.73 and 4.02–4.72, respectively.