bluuebunny/crossref_metadata_2025_split huggingface dataset:
The concept of destiny and free will in Chauntecleer's dream
Authors: Zuraikat Malek J. 
Published: October 2022

AbstractThe episode of Chauntecleer's dream found in Chaucer's "The Nun's Priest's Tale" is a controversial topic for Chaucer critics. Some critics argue that the downfall‐escape experience of Chauntecleer is worthy of investigation because animal figures in fables symbolize people (Finlayson, 2005, 495) and thus their rise and downfall sometimes allude to certain theological or philosophical issues such as the original Fall of Adam and Eve (Payne, 1976, 211–112). Other critics propose, on the contrary, that the episode of Chauntecleer is a "joke" and therefore should not be overestimated (Eliason, 1972, 172). These two opposing perspectives have their own rationale; nevertheless, the centrality of Chauntecleer's dream to the narrative of "The Nun's Priest's Tale" is hard to ignore. Considering the philosophical reverberations of the tale concerning the medieval concepts of destiny and free will, this paper argues that the Chauntecleer episode is not a "joke" but a philosophical exemplar that proposes the absence of any demarcating lines between destiny and free will, bearing in mind that destiny refers to the many options decreed by the deity to be available in every single situation for each individual, while free will refers to humans' freedom to decide what to say and do. The paper contends that Chauntecleer's dream shows how free will and destiny—though they are "inconsistent" forces (Boethius, 1785, 195)—mysteriously connive together to form what can be called conditional free will.
Factors Influencing Nonunion and Fracture Following Biological Intercalary Reconstruction for Lower‐Extremity Bone Tumors: A Systematic Review and Pooled Analysis
Authors: Huang Siyi , Li Hongfei , Xing Zhili , Ji Tao , Guo Wei 
Published: October 2022

ObjectivesTo determine nonunion rate, fracture rate, and their risk factors following biological intercalary reconstruction for lower extremity bone tumors.MethodsA systematic review and pooled analysis were conducted. PubMed, Embase, and Wiley Cochrane Library were searched from inception up to June 01, 2020. Studies concerning biological intercalary reconstruction after resection of lower extremity bone tumors were included. Overall nonunion and fracture rates were calculated. For studies reporting patient outcomes individually with precise graft characteristics and fixation methods, the individual data were extracted. Patients with demographical and clinical characteristics, including age, sex, tumor location, graft characteristics, and fixation method, were pooled for a multivariate analysis. For each factor of interest, odds ratio (OR), 95% confidence interval (95% CI), and p‐value from logistic regression were reported.ResultsA total of 2776 articles were identified from the initial literature search and 76 studies (2052 patients) were included. Sixty‐nine studies were case series and seven were comparative studies. The overall nonunion rate was 19% (382/2052; range: 0%–53%), and the overall fracture rate was 17% (344/2052; range: 0%–75%). Thirty of the 76 studies (362 patients) reported patients' characteristics individually and were thus included in the pooled multivariate analysis. Intramedullary nail fixation was associated with a significantly higher nonunion rate compared to plate fixation (OR = 2.2, 95% CI: 1.23–4.10, p = 0.009). Reconstruction with a vascularized fibula graft had a statistically non‐significant lower nonunion rate than reconstruction without the graft (OR = 0.6, 95% CI: 0.34–1.07, p = 0.086). Devitalized autografts had a lower fracture risk than allografts (OR = 0.3, 95% CI: 0.14–0.64, p = 0.002), and males tended to have higher fracture risk than females (OR = 2.1, 95% CI: 1.00–4.44, p = 0.049).ConclusionsReconstruction with intramedullary nail fixation is related to an elevated risk of nonunion. Allografts and males have a higher fracture risk than devitalized autografts and females, respectively. Further high‐quality comparative analyses with large sample sizes and adequate follow‐up duration are needed to validate these findings.
Clinical and molecular characteristics of autosomal recessive congenital ichthyosis in Thailand
Authors: Supsrisunjai Chavalit , Bunnag Thareena , Chaowalit Prapaipit , Boonpuen Niorn , Kootiratrakarn Tanawatt , Wessagowit Vesarat 
Published: October 2022

AbstractBackgroundAutosomal recessive congenital ichthyosis (ARCI) is a heterogenous group of rare keratinization disorders. To date, more than 13 causative genes have been identified. However, data on clinical and molecular characteristics including genotype–phenotype correlation are lacking in Thailand.ObjectiveWe collected cases diagnosed with non‐syndromic ARCI and syndromic recessive congenital ichthyosis at the Institute of Dermatology from 2011 to 2021 and performed genetic testing with next‐generation sequencing and assessed clinical details.MethodsBaseline demographic data, birth history, family history, skin manifestations at birth, current cutaneous manifestations, comorbidities, and response to treatments were assessed. DNA was screened for mutations using targeted gene sequencing of 45 genes related to congenital ichthyosis.ResultsA total of 33 patients were analyzed with an average age of 23.8 ± 13.9 years. Congenital ichthyosiform erythroderma (CIE) was most common (60.6%), followed by lamellar ichthyosis (18.2%), self‐improving congenital ichthyosis (6.1%), Netherton syndrome (6.1%), ichthyosis prematurity syndrome (3%), Sjögren–Larsson syndrome (3%) and bathing suit ichthyosis (3%). Eight genes were found with pathogenic variants in our cohort as follows: ABCA12 42.4% (14/33), NIPAL4 24.2% (8/33), TGM1 15.2% (5/33), SPINK5 6.1% (2/33), ALDH3A2 3% (1/33), SLC27A4 3% (1/33), CYP4F22 3% (1/33), and ST14 3% (1/33). Clinically, 79% of patients with ABCA12 pathogenic variants in this study had CIE, 79% of w had novel biallelic pathogenic compound heterozygous variants, whereas 21% had homozygous missense variants.ConclusionsThis is the first study to describe clinical and molecular findings of ARCI in a cohort from Thailand. Our findings demonstrate the clinical spectrum of the diseases and expand the molecular findings in a Southeast Asian population.
Effects of extender filtration and egg yolk concentration on canine semen cryopreservation
Authors: Divar Mohammad Reza , Mogheiseh Asghar , Mohammadi Fatemeh , Mavalizadeh Leili 
Published: October 2022

AbstractThe semen cooling and freezing extenders commonly contain the chicken egg yolk (EY) as the main sperm cryoprotectant. Besides its advantages, the EY has large lipoprotein granules that cause several physical and biological interferences. The previous studies have proposed several methods to resolve the problems with the EY‐based semen extenders, including mechanical agitation, EY fractionation, replacing the EY with purified EY LDL, and ultrasonication. In the current research, we aimed to evaluate the syringe filtration (220 nm) of an EY‐based canine semen freezing extender as a simple and cheap method to remove the EY granules. We also studied the possibility of re‐aggregation of EY granules after cooling, freeze/thawing, and lyophilization/rehydration of the filtered extenders. Additionally, we compared the effects of the filtration on lipid profile, turbidity, EY particle size distribution, and osmolality of the EY‐based extenders. Next, we examined the effects of filtered extenders containing different levels of EY (5%, 10%, 15%, 20%, and 25%) versus the control extender (20% EY, unfiltered) on post‐thaw sperm quality traits. We collected the semen samples from seven clinically healthy mixed‐breed adult dogs and pooled them for sperm freezing procedures. Samplings were repeated at least five times, independently. Our results indicated that the syringe filtration could remove the large EY particles and reduce the extender turbidity without affecting the lipid profile of the whole extender solution. The filtered extender supplemented with 25% (v/v) EY led to the best post‐thaw canine spermatozoa quality markers. The frozen–thawed spermatozoa evaluations included motility parameters (computer‐assisted semen analysis system), membrane and acrosome integrity (hypo‐osmotic swelling test, chlortetracycline binding assay), DNA fragmentation (sperm chromatin dispersion assay), membrane lipid peroxidation (MDA levels), apoptosis (Annexin V/propidium iodide assay), and fertility‐associated sperm mRNA transcript abundance (protamine 2 and 3). In conclusion, the syringe filtration of the EY‐based semen extenders was a simple and cheap method that could effectively remove large EY lipoprotein granules and possibly prevent EY‐origin bacterial contamination of the final extender solution. The EY at 25% (v/v) concentration in the filtered extenders resulted in the highest canine spermatozoa cryo‐tolerance.
Serologic evaluation of vaccine preventable infections and vaccination rates in kidney transplant candidates
Authors: Schneider Sabrina , Carlson Adrian , Sirandas Bhanupriya , Clark Breanna , Truax Crystal 
Published: October 2022

AbstractIntroductionAssessing vaccine serologic status presents opportunities to provide live vaccinations to kidney transplant candidates (KTC). This is especially important given the increased risk of infection while taking lifelong immunosuppression following transplant and the inability to routinely provide live vaccines to patients on immunosuppressive medications. In March 2019, the American Society of Transplantation Infectious Disease Community of Practice (AST‐IDCOP) released updated guidelines for vaccination of KTC, which emphasize pretransplant viral serology screening and live vaccine administration prior to transplant.Primary EndpointThe primary endpoint of this study was to determine adherence to AST‐IDCOP guidelines for live measles, mumps, and rubella (MMR) and VZV vaccination prior to transplant in KTC non‐immune by serology.MethodsThis retrospective, descriptive study examined serologic status and rates of live vaccination in 672 patients listed for kidney transplant at our center between July 2014 and July 2019. Secondary endpoints included subgroup analysis of adherence to full AST‐IDCOP vaccination recommendations and validation of CDC presumed immunity definitions for measles and VZV.ResultsSeventeen patients (2.7%) were nonimmune by serology for VZV, while 182 (27.1%) were nonimmune by serology to MMR. In a subgroup analysis of the seronegative KTC, none received VZV vaccination, and 6% received MMR vaccination prior to transplant or last follow‐up.ConclusionsOverall, a large portion of KTC had immunity gaps that were not resolved before transplantation. These findings are limited due to the retrospective, single‐center nature of this study and should be confirmed with larger, prospective assessments of serologic status and vaccine administration.image
Detection of intestinal parasites in human faecal samples using dissolved air flotation
Authors: Soares Felipe Augusto , dos Santos Bianca Martins , Rosa Stefany Laryssa , Loiola Saulo Hudson Nery , Stéfano Vitória Castilho , Proença Letícia Rodrigues , Suzuki Celso Tetsuo Nagase , Sabadini Edvaldo , Bresciani Katia Denise Saraiva , Falcão Alexandre Xavier , Gomes Jancarlo Ferreira 
Published: October 2022

AbstractObjectiveOva and parasite (O&P) examination is recommended for the laboratory diagnosis of agents causing parasitic infections; however, this exam requires scientific and technological improvements to enhance its diagnostic validity. Dissolved air flotation (DAF) is an efficient technical principle separating suspended solids in a liquid medium. We aimed to develop and validate a new procedure for intestinal parasite detection with DAF.MethodsIn this study, we collected samples from 500 volunteers, screened them by direct examination, and transferred the material to tubes using the Three Faecal Test (TF‐Test) for triplicate DAF tests. We evaluated physical–chemical parameters and DAF prototype components through quantifying parasites recovered from floated and non‐floated regions of the flotation column. The DAF operation protocol was validated with the gold standard results.ResultsThe 10% saturated volume proportion and cationic surfactant showed regularity and high parasite recovery (80%). Modifications of the needle device did not influence parasite recovery (p > 0.05). Sensitivity, specificity, accuracy and kappa agreement obtained with the DAF protocol were 91%, 100%, 93% and substantial (k = 0.64), respectively.ConclusionThe DAF principle could be used to process faecal samples in routine laboratory exams, enabling intestinal parasite detection.
CT and cross‐sectional anatomy of the paranasal sinuses in the Holstein cow
Authors: Turgut Nimet , Bahar Sadullah , Kılınçer Abidin 
Published: October 2022

AbstractThe structure of paranasal sinuses in cattle is difficult to understand due to its complexity, age‐related changes, and insufficient published data. In this prospective, anatomic study, we described the anatomy of the paranasal sinuses in the Holstein cow using computed tomography (CT) and cross‐sectional anatomic slices. Twelve healthy adult Holstein cow heads were used for this study. The heads were scanned using CT, and frozen anatomical sections were taken. The locations, borders, and relationships of the paranasal sinuses were defined on the anatomical sections and CT images. The paranasal sinuses on each side of the head consisted of conchal (dorsal, middle, and ventral), maxillary, lacrimal, palatine, frontal, sphenoid sinuses, and ethmoidal cells. The frontal sinus pneumatized all bones surrounding the cranial cavity, except for the ethmoidal and body of basisphenoid bones. The sphenoid and ventral conchal sinuses were the most asymmetrical, and the middle conchal sinus was the simplest. The ventral conchal sinus was detected in eleven animals, one of which was unilateral. This sinus communicated with the middle nasal meatus (13/21) and ventral nasal meatus (8/21). Findings can be used as background for interpreting CT studies of cattle with clinical signs of sinonasal region diseases. Future cross‐sectional radiological and reconstructive anatomical studies and investigation of the postnatal development of related structures in cattle are needed.
Refinement of a High Cycle Fatigue Decision-Gate Assessment for Additively Repaired Blades
Authors: Scott-Emuakpor Onome , Runyon Brian , Gillaugh Daniel , Smith Lucas , Johnson Phil 
Published: October 2022

Abstract
A recently developed high cycle fatigue (HCF) acceptance criteria demonstrates effectiveness in assessing the viability of two laser directed energy deposition (DED) repair processes on titanium 6Al-4V (Ti 6Al-4V) fan blades. The criteria demonstratively proved effective with basic coupon specimens; however, additional factors need to be considered to include available material or component information and nondestructive evaluation (NDE) conditional acceptance. The results from this study help define a prior rule (entrance statement) that identifies expected results based on manufacturing/microstructure information as well as a second-tier rule incorporating NDE for a more refined HCF acceptance criteria.
A Virtual Dual-Ripple Suppression Strategy of Double Fed Wind Turbine Under Wind Shear
Authors: Li Zhiyong , Pi Jiahua , Cao Yuan , Wang Xin 
Published: October 2022

Abstract
With the increasing size and scale of wind turbines, the ripple caused by wind shear may have negative effects on wind turbines, such as decreasing grid-connected power quality and increasing mechanical loss. To address this issue, a virtual dual-ripple suppression strategy is proposed to suppress the ripple caused by wind shear without additional cost and sacrificing system efficiency. First, in this paper, a three-bladed double-fed wind turbine is taken as the research object with the analysis of its transmission mechanism and form of ripple. Second, an online artificial neural network (ANN) ripple detection method is proposed to detect the time-varying low-frequency ripple with high accuracy. In addition, a virtual dual-ripple suppression strategy composed of two ANN-based filters is utilized to suppress electromagnetic torque ripple and grid-connected power ripple simultaneously. Finally, the accuracy of the presented ANN ripple detection method and suppression strategy are verified by matlab simulation. The results show that the virtual dual-ripple suppression strategy can effectively suppress the transmission of ripple while increasing the conversion efficiency of wind energy without additional hardware circuits and equipment.
Tribological and Friction-Induced Vibration Behaviors of Inorganic Fullerene-Like Tungsten Disulfide and Three-Dimensional Graphene as Lubricant Additives
Authors: Ouyang Tiancheng , Tang Wentao , Lei Wenwu , Cheng Liang 
Published: October 2022

Abstract
Friction and friction-induced vibration have a great impact on the service life of mechanical systems. In this study, the tribological performances and vibration characteristics of three-dimensional graphene nanosheets (3D GNS), inorganic fullerene-like tungsten disulfide (IF-WS2), and their mixed additive lubricants are investigated by experiments. The results show that IF-WS2/3D GNS (1:1) mixed additive can improve the friction-reduction and anti-wear of base oil by 62.9% and 71.4%, respectively. In terms of vibration damping, the mixed additive has an extremely strong dampening effect on vibration acceleration in all directions, especially in the Z-axis direction, where the amplitude is reduced by about 90%. The vibration damping should be attributed to the filling effect of 3D GNS and IF-WS2, which greatly flattens the friction surface, thus reducing the number of collision micro-bumps in the relative movement of the friction pair and suppressing the vibration. In addition, the unique ductile deformation of IF-WS2 makes the two additives form a flexible ball-bearing damper between the two contact surfaces, thus greatly reducing the vibration.
Preservice Physical Education Teacher Preparation for Health-Related Fitness Testing: A Faculty Perspective
Authors: Liu Xiaolu , Hamilton Xiaofen D. , Shangguan Rulan , Liu Jingwen , Wall Sarah J. , Guerra Richard 
Published: October 2022

Purpose: This study aimed to examine how physical education teacher education (PETE) programs prepare preservice physical education teachers (PPETs) for implementing health-related fitness testing (HRFT) in K–12 schools from a faculty perspective. Methods: The study applied a phenomenological approach. Seven PETE faculty participated in this study. Data were collected through semistructured interviews, online surveys, and course documents. Constant content analysis was performed to generate themes. Results: The study discovered four themes: (a) required courses are the primary means for preparing PPETs to use HRFT, (b) cooperating teachers can negatively influence the faculty’s role in HRFT preparation, (c) PPETs explored the use of HRFT in PETE programs through peer teaching and testing activities, and (d) there was a lack of field-based experiences in local schools for HRFT preparation. Conclusion: PETE programs provided some courses to prepare PPETs for the HRFT implementation with the lack of time and field experience.
Landscape of MicroRNA Regulatory Network Architecture and Functional Rerouting in Cancer
Authors: Hua Xu , Li Yongsheng , Pentaparthi Sairahul R. , McGrail Daniel J. , Zou Raymond , Guo Li , Shrawat Aditya , Cirillo Kara M. , Li Qing , Bhat Akshay , Xu Min , Qi Dan , Singh Ashok , McGrath Francis , Andrews Steven , Aung Kyaw Lwin , Das Jishnu , Zhou Yunyun , Lodi Alessia , Mills Gordon B. , Eckhardt S. Gail , Mendillo Marc L. , Tiziani Stefano , Wu Erxi , Huang Jason H. , Sahni Nidhi , Yi S. Stephen 
Published: October 2022

Abstract

Somatic mutations are a major source of cancer development, and many driver mutations have been identified in protein coding regions. However, the function of mutations located in miRNA and their target binding sites throughout the human genome remains largely unknown. Here, we built detailed cancer-specific miRNA regulatory networks across 30 cancer types to systematically analyze the effect of mutations in miRNAs and their target sites in 3′ untranslated region (3′ UTR), coding sequence (CDS), and 5′ UTR regions. A total of 3,518,261 mutations from 9,819 samples were mapped to miRNA–gene interactions (mGI). Mutations in miRNAs showed a mutually exclusive pattern with mutations in their target genes in almost all cancer types. A linear regression method identified 148 candidate driver mutations that can significantly perturb miRNA regulatory networks. Driver mutations in 3′UTRs played their roles by altering RNA binding energy and the expression of target genes. Finally, mutated driver gene targets in 3′ UTRs were significantly downregulated in cancer and functioned as tumor suppressors during cancer progression, suggesting potential miRNA candidates with significant clinical implications. A user-friendly, open-access web portal (mGI-map) was developed to facilitate further use of this data resource. Together, these results will facilitate novel noncoding biomarker identification and therapeutic drug design targeting the miRNA regulatory networks.

Significance:
A detailed miRNA–gene interaction map reveals extensive miRNA-mediated gene regulatory networks with mutation-induced perturbations across multiple cancers, serving as a resource for noncoding biomarker discovery and drug development.
N-myc–Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma
Authors: Kuzuoglu-Ozturk Duygu , Aksoy Ozlem , Schmidt Christin , Lea Robin , Larson Jon D. , Phelps Ryan R.L. , Nasholm Nicole , Holt Megan , Contreras Adrian , Huang Miller , Wong-Michalak Shannon , Shao Hao , Wechsler-Reya Robert , Phillips Joanna J. , Gestwicki Jason E. , Ruggero Davide , Weiss William A. 
Published: October 2022

Abstract

Deregulation of neuroblastoma-derived myc (N-myc) is a leading cause of malignant brain tumors in children. To target N-myc-driven medulloblastoma, most research has focused on identifying genomic alterations or on the analysis of the medulloblastoma transcriptome. Here, we have broadly characterized the translatome of medulloblastoma and shown that N-myc unexpectedly drives selective translation of transcripts that promote protein homeostasis. Cancer cells are constantly exposed to proteotoxic stress associated with alterations in protein production or folding. It remains poorly understood how cancers cope with proteotoxic stress to promote their growth. Here, our data revealed that N-myc regulates the expression of specific components (∼5%) of the protein folding machinery at the translational level through the major cap binding protein, eukaryotic initiation factor eIF4E. Reducing eIF4E levels in mouse models of medulloblastoma blocked tumorigenesis. Importantly, targeting Hsp70, a protein folding chaperone translationally regulated by N-myc, suppressed tumor growth in mouse and human medulloblastoma xenograft models. These findings reveal a previously hidden molecular program that promotes medulloblastoma formation and identify new therapies that may have impact in the clinic.

Significance:
Translatome analysis in medulloblastoma shows that N-myc drives selective translation of transcripts that promote protein homeostasis and that represent new therapeutic vulnerabilities.