bluuebunny/crossref_metadata_2025_split huggingface dataset:
Corticotropin-releasing Factor- Dopamine Interactions in Male and Female Macaque: Beyond the Classic Vta
Authors: Kelly E. A. , Fudge Julie L. 
Published: December 2022

Abstract
Dopamine (DA) is involved in stress and stress-related illnesses, including many psychiatric disorders. Corticotropin-releasing factor (CRF) plays a role in stress responses and targets the ventral midbrain DA system. This system is comprised of DA and non-DA cells and is divided into specific subregions. Although CRF inputs to the midline A10 nuclei of the DA system are well studied in rodents, in monkeys, CRF-containing terminals are also highly enriched in the expanded A10 parabrachial pigmented nucleus (PBP) and in the A8 retrorubral field subregion. In primates, the central extended amygdala, a rich source of CRF afferents across species, preferentially targets the PBP and A8 fields. We thus sought to characterize CRF terminals on DA (tyrosine hydroxylase, TH+) and non-DA (TH-) cell types in the PBP and A8 regions at the ultrastructural level using immuno-reactive electron microscopy (EM) for TH and CRF in male and female macaques. CRF labeling was present mostly in axon terminals, which mainly contacted non-DA dendrites in both subregions. Most CRF-positive terminals had inhibitory (symmetric) profiles. In the A8, CRF symmetric (inhibitory) contacts onto non-DA neurons were significantly greater than asymmetric (excitatory) profiles; this pattern was also seen in the PBP, but did not reach statistical significance. No sex differences were found. Hormonal assays suggested that our animals were at similar developmental stages and experienced similar stress levels. Together our findings suggest that at baseline, CRF terminals in the primate PBP and A8 largely regulate DA indirectly through non-DA neurons.
Accuracy requirements for the road friction coefficient estimation of a friction-adaptive automatic emergency steerassist (ESA)
Authors: Ahrenhold Tim , Jannes Iatropoulos , Roman Henze 
Published: December 2022

Abstract
The number of traffic accidents resulting in personal injury and property damage is increasingly beingreduced by effective advanced driver assistance systems (ADAS). Nevertheless, many traffic accidentsstill cannot be prevented today because they are due to wet, snow- and ice-covered roads. For this reason, the Institute of Automotive Engineering (IAE) of the Technical University of Braunschweigis investigating the road friction coefficient sensitivity and adaptation of advanced driver assistance systems (ADAS) currently in series production from 2018 to 2021 as part of the ‘Road Condition Cloud’ research project funded by the German Research Foundation (DFG) in order to increase driving safety,particularly on wet, snow- and ice-covered roads. In this article, the road friction coefficient sensitivityand adaptation of an automatic emergency steer assist is simulatively investigated. This assist overridesthe driver to automatically execute an evasive maneuver. The driving maneuver used is a standardizedobstacle-avoidance maneuver that is simulatively repeated on a dry, wet, snow- and ice-covered road.The road friction coefficient sensitivity shows that this test is already failed on a wet road because thesimulated vehicle does not pass the second lane without errors. Subsequently, a road friction coefficientadaptation of the emergency steer assist is investigated. This adaptation varies the maximum lateralacceleration of the evasive trajectory depending on an estimated value of the road friction coefficientin order not to exceed the maximum adhesion coefficient of the wheels during the evasive maneuver.Ideally, the estimated value matches the true road friction coefficient so that the second lane is passedwithout errors even on a wet, snow- and ice-covered road. In contrast, an existing difference determineswhether the test is passed. Finally, the necessary accuracy requirements of the road friction coefficientestimation are determined in an novel estimation error diagram. A road friction coefficient adaptationincreases the driving safety of driver advanced assistance systems (ADAS) that are in series productiontoday and future highly automated driving functions (HAF) and is necessary for automated drivingbecause the driver is not present as a fallback level.
Personalized circulating tumor DNA detection for monitoring immunotherapy efficacy in locally advanced or metastatic non-small cell lung cancer
Authors: Cheng Lei , Gao Guanghui , Zhao Chao , Wang Haowei , Yao Chao , Yu Hanchuanzhi , Yao Jichen , Li Feng , Guo Lijie , Jian Qijie , Chen Xiaoxia , Li Xuefei , Zhou Caicun 
Published: December 2022

Abstract
Immune checkpoint inhibitors (ICIs) or combined with chemotherapy have shown outstanding efficacy in treatment of advanced non-small cell lung cancer (NSCLC). However, efficient biomarkers for monitoring response to first-line ICIs ± chemotherapy remain unclear. In our study, 16 tumor tissues and 46 matched peripheral blood samples at baseline and during treatment from 19 locally advanced or metastatic NSCLC patients were collected. Circulating tumor DNA (ctDNA) burden by tumor-informed assay was detected to monitor and predict therapeutic response and survival of NSCLC patients treated with first-line ICIs or plus chemotherapy. We first compared the susceptibility of tumor-informed assay and tumor-agnostic assay to detect ctDNA, and found that ctDNA was only positively detected in one patient by tumor-agnostic assay with mean variant allele fraction (VAF) of 6.40%, while positively detected in three patients by tumor-informed assay with mean VAF of 8.83%, 0.154% and 0.176%, respectively. Moreover, tumor-informed assays demonstrated sensitive ctDNA detection in 93.75% (15/16) of patients. The trend of ctDNA level from baseline to first evaluation was consistent with radiographic changes. The decrease in ctDNA after treatment compared with baseline was more in patients with partial response than in patients with stable disease/progressive disease. Patients with more than 50% reduction had a significant progression-free survival and overall survival benefit. Our findings suggested that tumor-informed assay turned to be a better option for ctDNA detection, and plasma early ctDNA dynamic changes could be a valuable biomarker for monitoring efficacy and outcome prediction in advanced NSCLC patients treated with first-line ICIs ± chemotherapy.
Coupling magnetic levitation of graphene oxide-protein complexes with blood levels of glucose for early detection of pancreatic adenocarcinoma
Authors: Quagliarini Erica , Caputo Damiano , Cammarata Roberto , Caracciolo Giulio , Pozzi Daniela 
Published: December 2022

Abstract
Introduction
Pancreatic adenocarcinoma (PDAC) has a poor prognosis since often diagnosed too late. Dyslipidemia and hyperglycemia are considered risk factors, but the presence of the tumor itself can determine the onset of these disorders. Therefore, it is not easy to predict which subjects with diabetes or dyslipidemia will develop or have already developed PDAC. Over the past decade, tests based on the use of nanotechnology, alone or coupled with common laboratory tests (e.g., hemoglobin levels), have proven useful in aiding the diagnosis of PDAC. Tests based on magnetic levitation (MagLev) have demonstrated high diagnostic accuracy in compliance with the REASSURED criteria. Here we aimed to assess the ability of the MagLev test in detecting PDAC when coupled with the blood levels of glycemia, cholesterol, and triglycerides.
Methods
Blood samples from 24 PDAC patients and 22 healthy controls were collected. Human plasma was let to interact with graphene oxide (GO) nanosheets and the emerging coronated systems were put in the MagLev device. Outcomes from Maglev experiments were coupled to glycemia, cholesterol, and triglycerides levels. Linear discriminant analysis (LDA) was carried out to evaluate the classification ability of the test in terms of specificity, sensitivity, and global accuracy. Statistical analysis was performed with Matlab (MathWorks, Natick, MA, USA, Version R2022a) software.
Results
The positions of the levitating bands were measured at the starting point (i.e., as soon as the cuvette containing the sample was subjected to the magnetic field). Significant variations in the starting position of levitating nanosystems in controls and PDACs were detected. The combination of the MagLev outcomes with the blood glycemic levels returned the best value of global accuracy (91%) if compared to the coupling with those of cholesterol and triglycerides (global accuracy of ~77% and 84%, respectively).
Conclusion
If confirmed by further studies on larger cohorts, a multiplexed Maglev-based nanotechnology-enabled blood test could be employed as a screening tool for PDAC in populations with hyperglycemia.
Magnetothermal Effects and Critical Behavior of Rare Earth Manganese Oxides La 0.65 Ca 0.35 Mn 1-x Ni x O 3 (x=0, 0.2)
Authors: Zhao Jing , Zhao Jianjun , Jin Xiang , Yun Huiqin , Li Cheng , Hao Zhijun , Xing Ting , Ma Huaijin , Xing Ru 
Published: December 2022

Abstract
This study used the conventional solid-phase reaction method to prepare polycrystalline samples: La0.65Ca0.35Mn1−xNixO3 (x = 0, x = 0.2). The effects of Ni doping on the preformed cluster phases, magnetothermal effects, and critical behavior were systematically investigated. The results showed that the prepared polycrystalline samples all had cubic chalcogenide structures. The substitution of Ni2+ ions for a certain number of Mn3+ ions reduced the changes in the lattice parameters, unit cell volume, Curie temperature (Tc), magnetic entropy, and magneto-entropy. The changes in these properties originated from the partial substitution of Ni2+ for Mn3+ ions, which changed the Mn3+/Mn4+ ratio and decreased the Mn-O-Mn bond angle, thus weakening the double exchange interaction. Both samples had a preformed cluster phase above the low-temperature magnetic-transition temperature. The critical behaviors of both samples fit the tricritical model well. A transition from primary to secondary phase transition existed for both the parent and doped samples, and the maximum magnetic entropy changes of the samples at an applied magnetic field of 7 T were 7.70 J·kg− 1·K− 1 and 2.08 J·kg− 1·K− 1, respectively.
The vascular gene Apold1 is dispensable for normal development but controls angiogenesis under pathological conditions
Authors: Fan Zheng , Ardicoglu Raphaela , Batavia Aashil A. , Rust Ruslan , von Ziegler Lukas , Waag Rebecca , Zhang Jing , Desgeorges Thibaut , Sturman Oliver , Dang Hairuo , Weber Rebecca , Moor Andreas E. , Schwab Martin E. , Germain Pierre-Luc , Bohacek Johannes , De Bock Katrien 
Published: December 2022

Abstract
The molecular mechanisms of angiogenesis have been intensely studied, but many genes that control endothelial behavior and fate still need to be described. Here, we characterize the role of Apold1 (Apolipoprotein L domain containing 1) in angiogenesis in vivo and in vitro. Single-cell analyses reveal that - across tissues - the expression of Apold1 is restricted to the vasculature, and that Apold1 expression in endothelial cells (ECs) is highly sensitive to environmental factors. Using Apold1-/- mice, we find that Apold1 is dispensable for development and does not affect postnatal retinal angiogenesis nor alters the vascular network in adult brain and muscle. However, when exposed to ischemic conditions following photothrombotic stroke as well as femoral artery ligation, Apold1-/- mice display dramatic impairments in recovery and revascularization. We also find that human tumor endothelial cells express strikingly higher levels of Apold1, and that Apold1 deletion in mice stunts the growth of subcutaneous B16 melanoma tumors, which have smaller and poorly perfused vessels. Mechanistically, Apold1 is activated in ECs upon growth factor stimulation as well as in hypoxia, and Apold1 intrinsically controls EC proliferation but not migration. Our data demonstrate that Apold1 is a key regulator of angiogenesis in pathological settings, whereas it does not affect developmental angiogenesis, thus making it a promising candidate for clinical investigation.
Lysimeter Deep N Fertilizer Placement Reduced Leaching and Improved N Use Efficiency
Authors: Rychel Katrin , Meurer Katharina H.E. , Getahun Gizachew Tarekegn , Bergström Lars , Holger Kirchmann , Kätterer Thomas 
Published: December 2022

Abstract
Deep fertilization has been tested widely for nitrogen (N) use efficiency but there is little evidence of its impact on N leaching and the interplay between climate factors and crop N use. In this study, we tested the effect of three fertilizer N placements on greenhouse gas (GHG) emissions, leaching, and crop growth in a lysimeter experiment over a 33-month period comprised of three years with spring-sown cereals (S1, S2, and S3) with a preceding 11-month (F1) and a subsequent 15-month (F2) fallow period. In addition to a control with no N fertilizer (Control), 100 kg N ha− 1 year− 1 of ammonium nitrate was placed at 0.2 m (Deep), 0.07 m (Shallow), or halved between 0.07 m and 0.2 m (Mixed). Deep reduced leachate amount in each period except F1, with significant reductions (p < 0.05) in the drought year (S2) and cumulatively for S1-S3. Overall, Deep reduced water flow by 22, 25 and 34% compared to Shallow, Mixed and Control, respectively. Deep and Mixed reduced N leaching across S1-S3 compared with Shallow, but Deep further reduced N loads by 15% compared to Mixed and was significantly lowest (p < 0.05) among the fertilized treatments in S1 and S2. In S3, Deep increased grain yields by 28 and 22% compared to Shallow and Mixed, respectively, while maintaining a high grain N content and nearly doubling the agronomic efficiency of N (AEN) and the recovery efficiency of N (REN). Deep N placement is a promising mitigation practice that should be further investigated.
Sublingual Recurring Extra osseous Ewing Sarcoma: A Rare Case Report and Review of Literature
Authors: Alaroomy Leena ali , Amer Hatem , Ayad Haytham , El-din Yasmine Alaa , Alwadeai Mohamed 
Published: December 2022

Abstract
Background: Extera skeletal (Extraosseous) Ewing sarcoma is a rare primary tumor of the soft tissue, with an extremely high mortality rate and a bad prognosis which primarily affects young individuals. It occurs without particular clinical signs and can impact several different areas, which may delay diagnosis. Histopathological examination and immunohistochemistry are crucial for making a definite diagnosis. Besides this, early diagnosis is necessary for succeeding of the management.
Case report: The objective of the present case report was to document a rare case of EES which originated from the floor of the mouth. A 37-year-old male patient came to our clinic with sublingual rapidly growing swelling. He was suffering from difficulty in opening his mouth and a feeling of dysphagia. The patient revealed during a history-taking procedure that he had undergone one surgery to remove the mass that had previously developed in the same area, knowing that he had been diagnosed in the first as a non-specific inflammatory reaction. Clinical examinations revealed a dome shape soft tissue mass. The biopsy specimen underwent histopathological and immunohistochemical analysis, which demonstrated poorly differentiated small blue round cells with positive immuno-expression of CD-99, NKX2-2, FLI-1, and Vimentin. The final diagnosis was EES based on the clinical presentation, histopathological pattern, and findings of the immunohistochemistry investigations. Radiotherapy and chemotherapy were chosen as treatment modalities for the case. Unfortunately, the patient died after three months due to the occurrence of multiple distant metastasis areas.
Conclusion: Regardless of the relative rarity of Extraosseous Ewing sarcoma, it should be taken into account as a possible differential diagnosis for intraoral soft tissue masses, and accurate diagnosis with precise histopathological and immunohistochemical evaluations should be done as soon as possible. Moreover, improvement of management procedures of such cases generally provides the best chances for patient survival.
Developing a Parenting Model for the Decent Use of Digital Media among the Teenagers in Bangladesh: A Qualitative Exploration
Authors: BakiBillah Abul Hasan , Khan Ayub , Muhammad Faisal , Chowdhury ABM Alauddin , Shahinuzzaman Md. , Hossain Syed Billal , Akram Muhammad , Almasri Nihad A. , Wani Mohammad Amin , Chowdhury Moniruddin , Kabir Russell 
Published: December 2022

Abstract
Background: In Bangladesh, parents are crucial in ensuring that teens use digital gadgets responsibly. The purpose of this study was to create a parenting paradigm within the context of the government's purges.
Method: Nineteen key informants took part in this study as part of the qualitative inquiry.
Results: Participants come from a variety of backgrounds and play a variety of roles that all support the same goal. The parenting model was created using the information from the interviews. By placing secondary and upper secondary educational institutions at the core of government action, parents will be educated. This approach was first proposed to be used in the "Generation Breakthrough" program of the Ministry of Education. Parents should be invited to the institutions to learn about the actions that need to be performed and followed under the two main categories, preventative and curative. Technology, the spoken word, psychology, and relationships built on evidence and trust are all examples of preventive approaches. Corrective measures include counseling, assistance with cyber security, legal services, and law enforcement.
Conclusion: In order to ensure that teens are using digital gadgets responsibly, this study suggests educating and empowering parents through a realistic strategy.
The Zambian Energy Transition (ZET): A Case Study for OsemOSYS and FlexTool
Authors: Nyoni Kumbuso Joshua 
Published: December 2022

Abstract
This paper presents a Zambia study case for the application of Osemosys and Flextool. Three scenarios are explored assessing Zambian energy transition outlook (2022 – 2063) which include the business as usual (BAU), integrated resource plan (IRP), and Net Zero prospects to align with agenda 2063 for Africa. The output of Osemosys simulation modelling comprises detailed results providing capacity expansion planning of the generation assets to meet the anticipated demand for a specific range of future years (2022 – 2063). Based on the performance of the energy mix (i.e., hydro, thermal and renewable energy generation projects), the simulation assesses the adequacy of the available assets and identifies new capacity to be added at the least cost. Thereafter, the flexibility of the power system is assessed using FlexTool. To contextualise, FlexTool was used to assess the flexibility of the defined specific year of the capacity expansion plans produced in Osemosys. The writeup delved into analysis of three key assessment indicators for the various scenarios which include cost-effective generation, emissions reduction, and flexibility assessment outcome.
Genetic factors inherited from both diploid parents interact to affect genome stability and fertility in resynthesized allotetraploid Brassica napus
Authors: Katche Elizabeth Ihien , Schierholt Antje , Schiessl Sarah V. , He Fei , Lv Zhenling , Batley Jacqueline , Becker Heiko C. , Mason Annaliese S 
Published: December 2022

Abstract
Established allopolyploids are known to be genomically stable and fertile. However, by contrast, most newly resynthesized allopolyploids are infertile and meiotically unstable. Identifying the genetic factors responsible for genome stability in newly formed allopolyploid is key to understanding how two genomes come together to form a species. One hypothesis is that established allopolyploids may have inherited specific alleles from their diploid progenitors which conferred meiotic stability. Resynthesized B. napus lines are often unstable and infertile, unlike B. napus cultivars. We tested this hypothesis by characterizing 41 resynthesized B. napus lines produced by crosses between eight B. rapa and eight B. oleracea lines for copy number variation resulting from non-homologous recombination events, and fertility. We resequenced eight B. rapa and five B. oleracea parent accessions, and analyzed 19 resynthesized lines for allelic variation in a list of meiosis gene homologs. SNP genotyping was performed using the Illumina Infinium Brassica 60K array for three individuals per line. Self-pollinated seed-set and genome stability (number of copy number variants) were significantly affected by the interaction between both B. rapa and B. oleracea parental genotypes. We identified thirteen putative meiosis gene candidates which were significantly associated with frequency of copy number variants and which contained putatively harmful mutations in meiosis gene haplotypes for further investigation. Our results support the hypothesis that allelic variants inherited from parental genotypes affect genome stability and fertility in resynthesized rapeseed.
Retinopathy during the First 5 Years of Type 1 Diabetes and Subsequent Risk of Advanced Retinopathy
Authors: Malone John I. , Gao Xiaoyu , Raskin Philip , White Neil H. , Hainsworth Dean P. , Das Arup , Tamborlane William , Wallia Amisha , Aiello Lloyd P , Bebu Ionut 
Published: December 2022

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<p><u><em><strong>Objectives</strong></em></u><u><strong>:</strong></u> To determine whether individuals with type 1 diabetes (T1D) who develop any retinopathy at any time prior to five years of diabetes duration have an increased subsequent risk for further progression of retinopathy or onset of proliferative diabetic retinopathy (PDR), clinically significant macular edema (CSME), diabetes-related retinal photocoagulation or anti-VEGF injections. Additionally, to determine the influence of HbA1c and other risk factors in these individuals.</p>
<p><u><em><strong>Methods</strong></em></u><u><strong>:</strong></u> Diabetic retinopathy (DR) was assessed longitudinally using standardized stereoscopic seven-field fundus photography at time intervals of six months to four years. Early onset DR (EDR) was defined as onset prior to 5 years of T1D duration. Cox models assessed the associations of EDR with subsequent risk of outcomes.</p>
<p><u><em><strong>Results</strong></em></u><u><strong>:</strong></u> In unadjusted models, individuals with EDR (n=484) had an increased subsequent risk of PDR (hazard ratio HR=1.51, 95%CI [1.12,2.02], P=0.006), CSME (HR=1.44, [1.10,1.88], P=0.008) and diabetes-related retinal photocoagulation (HR=1.48, [1.12,1.96], P=0.006) compared to individuals without EDR (n=369). These associations remained significant adjusted for HbA1c, but only the association with PDR remained significant after adjustment for age, duration of T1D, HbA1c, sex, systolic/diastolic blood pressure, pulse, use of ACE inhibitors, albumin excretion rate, and eGFR (HR=1.47, [1.04,2.06], P=0.028). </p>
<p><u><em><strong>Conclusions</strong></em></u><u><strong>:</strong></u> These data suggest that individuals with any sign of retinopathy within the first 5 years of T1D onset may be at higher risk of long-term development of advanced DR, especially PDR. Identification of early onset DR may influence prognosis and help guide therapeutic management to reduce the risk of future visual loss in these individuals.</p>
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