bluuebunny/crossref_metadata_2025_split huggingface dataset:
ASSESSING THE 14C MARINE RESERVOIR EFFECT IN ARCHAEOLOGICAL CONTEXTS: DATA FROM THE CABEÇUDA SHELL MOUND IN SOUTHERN BRAZIL
Authors: Alves Eduardo Q , Macario Kita D , Scheel-Ybert Rita , Oliveira Fabiana M , Colonese André Carlo , Giannini Paulo César Fonseca , Guimarães Renato , Fallon Stewart , Muniz Marcelo , Chivall David , Bronk Ramsey Christopher 
Published: December 2022

ABSTRACTPrehistoric shell mounds can be useful for the quantification of the radiocarbon marine reservoir effect (MRE) and, at the same time, knowledge about the MRE allows for the establishment of robust chronologies for these sites. This creates a loop in which the archaeological setting has a dual role: it is part of both the method and the application. Therefore, it is paramount to address these sites from both archaeological and environmental perspectives, investigating their origin and diagenesis in order to overcome biases caused by post-depositional alterations. In this study, samples of bone, charcoal and shell from a Late Holocene shell mound in Southern Brazil, the Sambaqui de Cabeçuda, were analyzed following a multidisciplinary approach to disentangle the complex relationships between archaeology and the environment. We performed X-ray diffraction, radiocarbon dating, stable isotopes (δ13C, δ18O, δ15N) and anthracology analyses as well as Bayesian Chronological Models and Isotope Mixing Models to assess the local MRE and to reconstruct the diet of Cabeçuda builders. Our results reveal a negative local correction for the MRE (ΔR = –263 ± 46 14C yr), expected for the lagoon next to the site, and diets with considerable intakes of marine proteins. We examine the implications of these results for the chronology of the site and discuss a series of complications when performing MRE studies using shell mound sites.
Towards a Buddhist theism
Authors: Zappulli Davide Andrea 
Published: December 2022

AbstractMy claim in this article is that the thesis that Buddhism has no God, insofar as it is taken to apply to Buddhism universally, is false. I defend this claim by interpreting a central text in East-Asian Buddhism – The Awakening of Faith in Mahāyāna – through the lenses of perfect being theology (PBT), a research programme in philosophy of religion that attempts to provide a description of God through a two-step process: (1) defining God in terms of maximal greatness; (2) inferring the properties or attributes that God must have in virtue of satisfying the definition. My argument comprises two steps. First, I argue that, since PBT is a method for providing a description of God starting from a definition of God, any text that contains a PBT ipso facto contains a notion of God. Second, I argue through textual evidence that The Awakening articulates a PBT, concluding that it contains a notion of God. Since the method of PBT leaves open what descriptions are to be inferred, my argument allows me to conclude that a text contains a notion of God without previously committing to any particular conception of the divine, which makes it particularly versatile and powerful.
Anything Goes? Analyzing Varied Understandings of Assent
Authors: Birchley Giles 
Published: December 2022

AbstractAssent to medical research or treatment may be an intuitively attractive way to address the area between incapacity and capacity that might otherwise be subject to a best interests assessment. Assent has become a widely disseminated concept in law, research, and clinical ethics, but little conceptual work on assent has so far occurred. An exploration of use of assent in treatment and research in children and people with dementia suggests that at least five claims are made on behalf of assent. Since at least some of these may lead to tensions with others, assent requires firmer conceptual underpinning. Whether assent remains primarily a local approach to research in children in the United States, where it appears to fit with legal background conditions, or develops its promise to overturn the dominant, binary, approach to (in)capacity will depend on the strength of future philosophical work to improve the theoretical foundations to assent.
FUNDC1 protects against doxorubicin-induced cardiomyocyte PANoptosis through stabilizing mtDNA via interaction with TUFM
Authors: Bi Yaguang , Xu Haixia , Wang Xiang , Zhu Hong , Ge Junbo , Ren Jun , Zhang Yingmei 
Published: December 2022

AbstractDoxorubicin (DOX) is an effective anthracycline chemotherapeutic anticancer drug with its life-threatening cardiotoxicity severely limiting its clinical application. Mitochondrial damage-induced cardiomyocyte death is considered an essential cue for DOX cardiotoxicity. FUN14 domain containing 1 (FUNDC1) is a mitochondrial membrane protein participating in the regulation of mitochondrial integrity in multiple diseases although its role in DOX cardiomyopathy remains elusive. Here, we examined whether PANoptosis, a novel type of programmed cell death closely associated with mitochondrial damage, was involved in DOX-induced heart injury, and FUNDC1-mediated regulation of cardiomyocyte PANoptosis, if any. FUNDC1 was downregulated in heart tissues in patients with dilated cardiomyopathy (DCM) and DOX-challenged mice. FUNDC1 deficiency aggravated DOX-induced cardiac dysfunction, mitochondrial injury, and cardiomyocyte PANoptosis. Further examination revealed that FUNDC1 countered cytoplasmic release of mitochondrial DNA (mtDNA) and activation of PANoptosome through interaction with mitochondrial Tu translation elongation factor (TUFM), a key factor in the translational expression and repair of mitochondrial DNA, via its 96–133 amino acid domain. TUFM intervention reversed FUNDC1-elicited protection against DOX-induced mtDNA cytosolic release and cardiomyocyte PANoptosis. Our findings shed light toward a beneficial role of FUNDC1 in DOX cardiotoxicity and cardiomyocyte PANoptosis, thus offering therapeutic promises in DOX-induced cardiotoxicity.
Extracellular CIRP dysregulates macrophage bacterial phagocytosis in sepsis
Authors: Zhou Mian , Aziz Monowar , Yen Hao-Ting , Ma Gaifeng , Murao Atsushi , Wang Ping 
Published: December 2022

AbstractIn sepsis, macrophage bacterial phagocytosis is impaired, but the mechanism is not well elucidated. Extracellular cold-inducible RNA-binding protein (eCIRP) is a damage-associated molecular pattern that causes inflammation. However, whether eCIRP regulates macrophage bacterial phagocytosis is unknown. Here, we reported that the bacterial loads in the blood and peritoneal fluid were decreased in CIRP−/− mice and anti-eCIRP Ab-treated mice after sepsis. Increased eCIRP levels were correlated with decreased bacterial clearance in septic mice. CIRP−/− mice showed a marked increase in survival after sepsis. Recombinant murine CIRP (rmCIRP) significantly decreased the phagocytosis of bacteria by macrophages in vivo and in vitro. rmCIRP decreased the protein expression of actin-binding proteins, ARP2, and p-cofilin in macrophages. rmCIRP significantly downregulated the protein expression of βPIX, a Rac1 activator. We further demonstrated that STAT3 and βPIX formed a complex following rmCIRP treatment, preventing βPIX from activating Rac1. We also found that eCIRP-induced STAT3 phosphorylation was required for eCIRP’s action in actin remodeling. Inhibition of STAT3 phosphorylation prevented the formation of the STAT3-βPIX complex, restoring ARP2 and p-cofilin expression and membrane protrusion in rmCIRP-treated macrophages. The STAT3 inhibitor stattic rescued the macrophage phagocytic dysfunction induced by rmCIRP. Thus, we identified a novel mechanism of macrophage phagocytic dysfunction caused by eCIRP, which provides a new therapeutic target to ameliorate sepsis.
Constraining surface properties of asteroid (162173) Ryugu from numerical simulations of Hayabusa2 mission impact experiment
Authors: Jutzi Martin , Raducan Sabina D. , Zhang Yun , Michel Patrick , Arakawa Masahiko 
Published: December 2022

AbstractThe Hayabusa2 mission impact experiment on asteroid Ryugu created an unexpectedly large crater. The associated regime of low-gravity, low-strength cratering remained largely unexplored so far, because these impact conditions cannot be re-created in laboratory experiments on Earth. Here we show that the target cohesion may be very low and the impact probably occurred in the transitional cratering regime, between strength and gravity. For such conditions, our numerical simulations are able to reproduce the outcome of the impact on Ryugu, including the effects of boulders originally located near the impact point. Consistent with most recent analysis of Ryugu and Bennu, cratering scaling-laws derived from our results suggest that surfaces of small asteroids must be very young. However, our results also show that the cratering efficiency can be strongly affected by the presence of a very small amount of cohesion. Consequently, the varying ages of different geological surface units on Ryugu may be due to the influence of cohesion.
Genome-wide association study of REM sleep behavior disorder identifies polygenic risk and brain expression effects
Authors: Krohn Lynne , Heilbron Karl , Blauwendraat Cornelis , Reynolds Regina H. , Yu Eric , Senkevich Konstantin , Rudakou Uladzislau , Estiar Mehrdad A. , Gustavsson Emil K. , Brolin Kajsa , Ruskey Jennifer A. , Freeman Kathryn , Asayesh Farnaz , Chia Ruth , Arnulf Isabelle , Hu Michele T. M. , Montplaisir Jacques Y. , Gagnon Jean-François , Desautels Alex , Dauvilliers Yves , Gigli Gian Luigi , Valente Mariarosaria , Janes Francesco , Bernardini Andrea , Högl Birgit , Stefani Ambra , Ibrahim Abubaker , Šonka Karel , Kemlink David , Oertel Wolfgang , Janzen Annette , Plazzi Giuseppe , Biscarini Francesco , Antelmi Elena , Figorilli Michela , Puligheddu Monica , Mollenhauer Brit , Trenkwalder Claudia , Sixel-Döring Friederike , Cochen De Cock Valérie , Monaca Christelle Charley , Heidbreder Anna , Ferini-Strambi Luigi , Dijkstra Femke , Viaene Mineke , Abril Beatriz , Boeve Bradley F. ,   23andMe Research Team, Aslibekyan Stella , Auton Adam , Babalola Elizabeth , Bell Robert K. , Bielenberg Jessica , Bryc Katarzyna , Bullis Emily , Coker Daniella , Partida Gabriel Cuellar , Dhamija Devika , Das Sayantan , Elson Sarah L. , Filshtein Teresa , Fletez-Brant Kipper , Fontanillas Pierre , Freyman Will , Gandhi Pooja M. , Hicks Barry , Hinds David A. , Jewett Ethan M. , Jiang Yunxuan , Kukar Katelyn , Lin Keng-Han , Lowe Maya , McCreight Jey C. , McIntyre Matthew H. , Micheletti Steven J. , Moreno Meghan E. , Mountain Joanna L. , Nandakumar Priyanka , Noblin Elizabeth S. , O’Connell Jared , Petrakovitz Aaron A. , Poznik G. David , Schumacher Morgan , Shastri Anjali J. , Shelton Janie F. , Shi Jingchunzi , Shringarpure Suyash , Tran Vinh , Tung Joyce Y. , Wang Xin , Wang Wei , Weldon Catherine H. , Wilton Peter , Hernandez Alejandro , Wong Corinna , Tchakouté Christophe Toukam , Scholz Sonja W. , Ryten Mina , Bandres-Ciga Sara , Noyce Alastair , Cannon Paul , Pihlstrøm Lasse , Nalls Mike A. , Singleton Andrew B. , Rouleau Guy A. , Postuma Ronald B. , Gan-Or Ziv 
Published: December 2022

AbstractRapid-eye movement (REM) sleep behavior disorder (RBD), enactment of dreams during REM sleep, is an early clinical symptom of alpha-synucleinopathies and defines a more severe subtype. The genetic background of RBD and its underlying mechanisms are not well understood. Here, we perform a genome-wide association study of RBD, identifying five RBD risk loci near SNCA, GBA, TMEM175, INPP5F, and SCARB2. Expression analyses highlight SNCA-AS1 and potentially SCARB2 differential expression in different brain regions in RBD, with SNCA-AS1 further supported by colocalization analyses. Polygenic risk score, pathway analysis, and genetic correlations provide further insights into RBD genetics, highlighting RBD as a unique alpha-synucleinopathy subpopulation that will allow future early intervention.
Spatially resolved proteomics via tissue expansion
Authors: Li Lu , Sun Cuiji , Sun Yaoting , Dong Zhen , Wu Runxin , Sun Xiaoting , Zhang Hanbin , Jiang Wenhao , Zhou Yan , Cen Xufeng , Cai Shang , Xia Hongguang , Zhu Yi , Guo Tiannan , Piatkevich Kiryl D. 
Published: December 2022

AbstractSpatially resolved proteomics is an emerging approach for mapping proteome heterogeneity of biological samples, however, it remains technically challenging due to the complexity of the tissue microsampling techniques and mass spectrometry analysis of nanoscale specimen volumes. Here, we describe a spatially resolved proteomics method based on the combination of tissue expansion with mass spectrometry-based proteomics, which we call Expansion Proteomics (ProteomEx). ProteomEx enables quantitative profiling of the spatial variability of the proteome in mammalian tissues at ~160 µm lateral resolution, equivalent to the tissue volume of 0.61 nL, using manual microsampling without the need for custom or special equipment. We validated and demonstrated the utility of ProteomEx for streamlined large-scale proteomics profiling of biological tissues including brain, liver, and breast cancer. We further applied ProteomEx for identifying proteins associated with Alzheimer’s disease in a mouse model by comparative proteomic analysis of brain subregions.
Impact of international travel and diarrhea on gut microbiome and resistome dynamics
Authors: Boolchandani Manish , Blake Kevin S. , Tilley Drake H. , Cabada Miguel M. , Schwartz Drew J. , Patel Sanket , Morales Maria Luisa , Meza Rina , Soto Giselle , Isidean Sandra D. , Porter Chad K. , Simons Mark P. , Dantas Gautam 
Published: December 2022

AbstractInternational travel contributes to the global spread of antimicrobial resistance. Travelers’ diarrhea exacerbates the risk of acquiring multidrug-resistant organisms and can lead to persistent gastrointestinal disturbance post-travel. However, little is known about the impact of diarrhea on travelers’ gut microbiomes, and the dynamics of these changes throughout travel. Here, we assembled a cohort of 159 international students visiting the Andean city of Cusco, Peru and applied next-generation sequencing techniques to 718 longitudinally-collected stool samples. We find that gut microbiome composition changed significantly throughout travel, but taxonomic diversity remained stable. However, diarrhea disrupted this stability and resulted in an increased abundance of antimicrobial resistance genes that can remain high for weeks. We also identified taxa differentially abundant between diarrheal and non-diarrheal samples, which were used to develop a classification model that distinguishes between these disease states. Additionally, we sequenced the genomes of 212 diarrheagenic Escherichia coli isolates and found those from travelers who experienced diarrhea encoded more antimicrobial resistance genes than those who did not. In this work, we find the gut microbiomes of international travelers’ are resilient to dysbiosis; however, they are also susceptible to colonization by multidrug-resistant bacteria, a risk that is more pronounced in travelers with diarrhea.
The circadian clock influences T cell responses to vaccination by regulating dendritic cell antigen processing
Authors: Cervantes-Silva Mariana P. , Carroll Richard G. , Wilk Mieszko M. , Moreira Diana , Payet Cloe A. , O’Siorain James R. , Cox Shannon L. , Fagan Lauren E. , Klavina Paula A. , He Yan , Drewinski Tabea , McGinley Alan , Buel Sharleen M. , Timmons George A. , Early James O. , Preston Roger J. S. , Hurley Jennifer M. , Finlay David K. , Schoen Ingmar , Javier Sánchez-García F. , Mills Kingston H. G. , Curtis Annie M. 
Published: December 2022

AbstractDendritic cells play a key role in processing and presenting antigens to naïve T cells to prime adaptive immunity. Circadian rhythms are known to regulate many aspects of immunity; however, the role of circadian rhythms in dendritic cell function is still unclear. Here, we show greater T cell responses when mice are immunised in the middle of their rest versus their active phase. We find a circadian rhythm in antigen processing that correlates with rhythms in both mitochondrial morphology and metabolism, dependent on the molecular clock gene,Bmal1. Using Mdivi-1, a compound that promotes mitochondrial fusion, we are able to rescue the circadian deficit in antigen processing and mechanistically link mitochondrial morphology and antigen processing. Furthermore, we find that circadian changes in mitochondrial Ca2+are central to the circadian regulation of antigen processing. Our results indicate that rhythmic changes in mitochondrial calcium, which are associated with changes in mitochondrial morphology, regulate antigen processing.
Air pollution disparities and equality assessments of US national decarbonization strategies
Authors: Goforth Teagan , Nock Destenie 
Published: December 2022

AbstractEnergy transitions and decarbonization require rapid changes to a nation’s electricity generation mix. There are many feasible decarbonization pathways for the electricity sector, yet there is vast uncertainty about how these pathways will advance or derail the nation’s energy equality goals. We present a framework for investigating how decarbonization pathways, driven by a least-cost paradigm, will impact air pollution inequality across vulnerable groups (e.g., low-income, minorities) in the US. We find that if no decarbonization policies are implemented, Black and high-poverty communities may be burdened with 0.19–0.22 μg/m3 higher PM2.5 concentrations than the national average during the energy transition. National mandates requiring more than 80% deployment of renewable or low-carbon technologies achieve equality of air pollution concentrations across all demographic groups. Thus, if least-cost optimization capacity expansion models remain the dominant decision-making paradigm, strict low-carbon or renewable energy technology mandates will have the greatest likelihood of achieving national distributional energy equality. Decarbonization is essential to achieving climate goals, but myopic decarbonization policies that ignore co-pollutants may leave Black and high-poverty communities up to 26–34% higher PM2.5 exposure than national averages over the energy transition.
Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light
Authors: Oikawa Kazusato , Goto-Yamada Shino , Hayashi Yasuko , Takahashi Daisuke , Kimori Yoshitaka , Shibata Michitaro , Yoshimoto Kohki , Takemiya Atsushi , Kondo Maki , Hikino Kazumi , Kato Akira , Shimoda Keisuke , Ueda Haruko , Uemura Matsuo , Numata Keiji , Ohsumi Yoshinori , Hara-Nishimura Ikuko , Mano Shoji , Yamada Kenji , Nishimura Mikio 
Published: December 2022

AbstractAlthough light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation.
Single-cell sequencing shows cellular heterogeneity of cutaneous lesions in lupus erythematosus
Authors: Zheng Meiling , Hu Zhi , Mei Xiaole , Ouyang Lianlian , Song Yang , Zhou Wenhui , Kong Yi , Wu Ruifang , Rao Shijia , Long Hai , Shi Wei , Jing Hui , Lu Shuang , Wu Haijing , Jia Sujie , Lu Qianjin , Zhao Ming 
Published: December 2022

AbstractDiscoid lupus erythematosus (DLE) and systemic lupus erythematosus (SLE) are both types of lupus, yet the characteristics, and differences between them are not fully understood. Here we show single-cell RNA sequencing data of cutaneous lesions from DLE and SLE patients and skin tissues from healthy controls (HCs). We find significantly higher proportions of T cells, B cells and NK cells in DLE than in SLE. Expanded CCL20+ keratinocyte, CXCL1+ fibroblast, ISGhiCD4/CD8 T cell, ISGhi plasma cell, pDC, and NK subclusters are identified in DLE and SLE compared to HC. In addition, we observe higher cell communication scores between cell types such as fibroblasts and macrophage/dendritic cells in cutaneous lesions of DLE and SLE compared to HC. In summary, we clarify the heterogeneous characteristics in cutaneous lesions between DLE and SLE, and discover some specific cell subtypes and ligand-receptor pairs that indicate possible therapeutic targets of lupus erythematosus.
High-throughput screening of 2D van der Waals crystals with plastic deformability
Authors: Gao Zhiqiang , Wei Tian-Ran , Deng Tingting , Qiu Pengfei , Xu Wei , Wang Yuecun , Chen Lidong , Shi Xun 
Published: December 2022

AbstractInorganic semiconductors exhibit multifarious physical properties, but they are prevailingly brittle, impeding their application in flexible and hetero-shaped electronics. The exceptional plasticity discovered in InSe crystal indicates the existence of abundant plastically deformable two-dimensional van der Waals (2D vdW) materials, but the conventional trial-and-error method is too time-consuming and costly. Here we report on the discovery of tens of potential 2D chalcogenide crystals with plastic deformability using a nearly automated and efficient high-throughput screening methodology. Seven candidates e.g., famous MoS2, GaSe, and SnSe2 2D materials are carefully verified to show largely anisotropic plastic deformations, which are contributed by both interlayer and cross-layer slips involving continuous breaking and reconstruction of chemical interactions. The plasticity becomes a new facet of 2D materials for deformable or flexible electronics.
Toward economical application of carbon capture and utilization technology with near-zero carbon emission
Authors: Langie Kezia Megagita Gerby , Tak Kyungjae , Kim Changsoo , Lee Hee Won , Park Kwangho , Kim Dongjin , Jung Wonsang , Lee Chan Woo , Oh Hyung-Suk , Lee Dong Ki , Koh Jai Hyun , Min Byoung Koun , Won Da Hye , Lee Ung 
Published: December 2022

AbstractCarbon capture and utilization technology has been studied for its practical ability to reduce CO2 emissions and enable economical chemical production. The main challenge of this technology is that a large amount of thermal energy must be provided to supply high-purity CO2 and purify the product. Herein, we propose a new concept called reaction swing absorption, which produces synthesis gas (syngas) with net-zero CO2 emission through direct electrochemical CO2 reduction in a newly proposed amine solution, triethylamine. Experimental investigations show high CO2 absorption rates (>84%) of triethylamine from low CO2 concentrated flue gas. In addition, the CO Faradaic efficiency in a triethylamine supplied membrane electrode assembly electrolyzer is approximately 30% (@−200 mA cm−2), twice higher than those in conventional alkanolamine solvents. Based on the experimental results and rigorous process modeling, we reveal that reaction swing absorption produces high pressure syngas at a reasonable cost with negligible CO2 emissions. This system provides a fundamental solution for the CO2 crossover and low system stability of electrochemical CO2 reduction.