bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of novel DNA marker for species discrimination of Fasciola flukes based on the fatty acid binding protein type I gene
Authors: Okamoto Emi , Tashiro Michiyo , Ortiz Pedro , Mohanta Uday Kumar , Hobán Cristian , Murga-Moreno César A. , Angulo-Tisoc José M. , Ichikawa-Seki Madoka 
Published: October 2022

Abstract
Background
Multiplex polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) for nuclear phosphoenolpyruvate carboxykinase (pepck) and polymerase delta (pold), respectively, have been used to differentiate Fasciola hepatica, F. gigantica, and hybrid Fasciola flukes. However, discrimination errors have been reported in both methods. This study aimed to develop a multiplex PCR based on a novel nuclear marker, the fatty acid binding protein type I (FABP) type I gene.

Methods
Nucleotide sequence variations of FABP type I were analyzed using DNA samples of F. hepatica, F. gigantica, and hybrid Fasciola flukes obtained from 11 countries in Europe, Latin America, Africa, and Asia. A common forward primer for F. hepatica and F. gigantica and two specific reverse primers for F. hepatica and F. gigantica were designed for multiplex PCR.

Results
Specific fragments of F. hepatica (290 bp) and F. gigantica (190 bp) were successfully amplified using multiplex PCR. However, the hybrid flukes contained fragments of both species. The multiplex PCR for FABP type I could precisely discriminate the 1312 Fasciola samples used in this study. Notably, no discrimination errors were observed with this novel method.

Conclusions
Multiplex PCR for FABP type I can be used as a species discrimination marker in place of pepck and pold. The robustness of the species-specific primer should be continuously examined using a larger number of Fasciola flukes worldwide in the future since nucleotide substitutions in the primer regions may cause amplification errors.

Graphical abstract
Computational quantification and characterization of independently evolving cellular subpopulations within tumors is critical to inhibit anti-cancer therapy resistance
Authors: Alkhatib Heba , Rubinstein Ariel M. , Vasudevan Swetha , Flashner-Abramson Efrat , Stefansky Shira , Chowdhury Sangita Roy , Oguche Solomon , Peretz-Yablonsky Tamar , Granit Avital , Granot Zvi , Ben-Porath Ittai , Sheva Kim , Feldman Jon , Cohen Noa E. , Meirovitz Amichay , Kravchenko-Balasha Nataly 
Published: October 2022

Abstract
Background
Drug resistance continues to be a major limiting factor across diverse anti-cancer therapies. Contributing to the complexity of this challenge is cancer plasticity, in which one cancer subtype switches to another in response to treatment, for example, triple-negative breast cancer (TNBC) to Her2-positive breast cancer. For optimal treatment outcomes, accurate tumor diagnosis and subsequent therapeutic decisions are vital. This study assessed a novel approach to characterize treatment-induced evolutionary changes of distinct tumor cell subpopulations to identify and therapeutically exploit anticancer drug resistance.

Methods
In this research, an information-theoretic single-cell quantification strategy was developed to provide a high-resolution and individualized assessment of tumor composition for a customized treatment approach.
Briefly, this single-cell quantification strategy computes cell barcodes based on at least 100,000 tumor cells from each experiment and reveals a cell-specific signaling signature (CSSS) composed of a set of ongoing processes in each cell.

Results
Using these CSSS-based barcodes, distinct subpopulations evolving within the tumor in response to an outside influence, like anticancer treatments, were revealed and mapped. Barcodes were further applied to assign targeted drug combinations to each individual tumor to optimize tumor response to therapy.
The strategy was validated using TNBC models and patient-derived tumors known to switch phenotypes in response to radiotherapy (RT).

Conclusions
We show that a barcode-guided targeted drug cocktail significantly enhances tumor response to RT and prevents regrowth of once-resistant tumors. The strategy presented herein shows promise in preventing cancer treatment resistance, with significant applicability in clinical use.
Identification and growth-promoting effect of endophytic bacteria in potato
Authors: Shuang Ma , Sun Jiying , Teng Wang 
Published: October 2022

AbstractIn agriculture, Bacillus
species are efficient and ecologically tool for promote the growth of the
plant.Purpose: This study obtains the plant growth-promoting (PGP) ability of
endophytic bacteria isolated from the potato tubers.Methods: Using endophytic
bacteria to promote potato growth, achieve the purpose of increasing production.
In this experiment, the growth- promoting ability of the strain was verified by
laboratory identification and field test validation.Result: The isolates
were identified as Bacillus species based on a 16S rRNA gene sequence
and gyrB gene sequence analysis. DNA hybridization finally identified it as Bacillus
velezensis. Among the PGP attributes, the strain K-9 was found to be positive
for indole acetic acid (IAA) production, phosphate solubilization, siderophore
production, and nitrogen fixation. The isolate was found negative for potassium
solubilization. The quantitative estimation of IAA product to 9.09 μg/ml. The
isolate also had the ability to produce lytic enzymes such as amylase and
protease. The quantitative estimation of protease activity is 89.16 μg/ml. The inoculation strain K-9 improved bioaccumulation of roots
and buds and yield in the potato compared to uninoculated control plants.Conclusion: These findings give an insight into the ways to use PGP bacteria to increase
potato production.
Quantitative MRI indicators and features for partial subscapularis tendon tears on conventional shoulder MRI
Authors: Wang Qiqi , Zhao Jie , Zhou Suying , Lv Yuchan , Liu Xin , Yang Haitao 
Published: October 2022

Abstract
Background
Diagnosing partial subscapularis (SSC) tendon tears still faces challenges. A failure rate of massive posterosuperior rotator cuff tear repair will be highly increased when extending more than one-third of SSC tendon. This study aims to investigate the quantitative indicators and features of partial SSC tears on conventional shoulder MRI and improve the preoperative diagnostic accuracy.

Materials and methods
Four hundred and thirty-seven patients underwent MRI and arthroscopy were retrospectively reviewed; 89 patients with partial SSC tears in case group and 50 patients with normal SSC in control group were included. Six MRI features with the explicit definition of some quantitative indicators were evaluated.

Results
Fissure sign showed the highest diagnostic efficiency for the partial SSC tears, with a specificity of 92%, sensitivity of 75.3%, and accuracy of 81.3%. Thinning of SSC, fluid collection under the coraco-glenoid arch (CGA), and combined SSP complete tear also showed high specificity of 86%, 80%, and 80%, respectively, while the sensitivity and accuracy were moderate, with a sensitivity of 38.2%, 50.6%, and 48.3%, respectively, an accuracy of 55.4%, 61.2%, and 59.7%, respectively. The specificity, sensitivity, and accuracy of lesser tuberosity cysts were all moderate with values of 68%, 56.2%, and 60%, respectively. However, fat accumulation under the CGA showed no significant difference between the partial SSC tears group and the control group.

Conclusion
Several specific MRI features with quantitative indicators defined in this study can be used to improve the accuracy of preoperative MRI diagnosis of partial SSC tears.
Sustained remission of psychotic symptoms secondary to hypothyroidism (myxedema psychosis) after 6 months of treatment primarily with levothyroxine: a case report
Authors: Chan Eric C. 
Published: October 2022

Abstract
Background
Psychotic symptoms associated with hypothyroidism, also known as “myxedema psychosis,” are a treatable cause of psychosis often associated with complete recovery. While most cases receive both thyroxine and a short course of antipsychotics, some reports indicate that symptoms can resolve without antipsychotic treatment, though follow-up in these cases has often been short or not reported. This is one of the first case reports demonstrating sustained remission of psychotic symptoms at 6 months in a case of myxedema psychosis treated with minimal antipsychotic medication.

Case presentation
We describe the case of a 40-year-old Caucasian woman who was brought to hospital with a 7-day history of anxiety and decreased sleep and 1 day of disorganized speech, paranoid delusions, and auditory hallucinations. After being admitted to psychiatry for management, screening blood work revealed elevated thyroid-stimulating hormone. The patient was initiated on treatment with levothyroxine and low doses of antipsychotics. Her symptoms resolved on the third day of her admission with ongoing symptomatic remission at 6 months follow-up.

Conclusions
The identification of myxedema psychosis is important owing to the implications on treatment and prognosis of the disorder. Our case suggests that sustained symptom resolution may occur with little to no antipsychotic treatment, though these findings are preliminary and additional study is needed before definitive conclusions on the optimal approach can be made.
Association of co-occurring mental health problems with hepatitis C status among young people who inject drugs in rural New Mexico, 2016–2018
Authors: Gupta Akash , Shebl Fatma M. , Tong Yao , Wagner Katherine , Bassett Ingrid V. , Page Kimberly , Winstanley Erin L. 
Published: October 2022

Abstract
Background
Injection drug use (IDU) remains the strongest risk factor for hepatitis C virus (HCV) in the United States. HCV rates are increasing in rural areas among young adult people who inject drugs (PWID). People with HCV and PWID have disproportionate rates of mental health problems; however, it is unclear whether risky injection behaviors mediate the association between mental health problems and HCV. We examined the association between mental health problems and HCV in a rural cohort of young adult PWID, with the goal of informing rural service delivery.

Methods
We conducted a secondary analysis of cross-sectional data from a convenience sample of young adult PWID in 2 rural counties in New Mexico. Participants were recruited from 2 community venues between September 2016 and May 2018. Associations between mental health problems and HCV were examined using bivariate (Fisher’s exact) and multivariable modified Poisson regression analyses (with robust standard errors). Using structural equation modeling (SEM), we assessed duration of IDU and receptive syringe sharing (RSS) as mediators of this relationship.

Results
A total of 263 patients were enrolled, with a median age of 26.1 years. The majority were male (66.3%) and Hispanic/Latino (a) (87.6%). The median age first injected was 19 years, and over half reported having ever engaged in RSS (53.4%). At least one mental health problem was reported by 60.1% of participants, with post-traumatic stress disorder (PTSD) being the most prevalent condition (42.2%). A majority (60.9%) tested positive for HCV antibody, and just under half (45.7%) of all participants tested positive for HCV ribonucleic acid. In SEM, PTSD had a significant total effect on HCV (τ = 0.230, P = 0.05), and this relationship was partially mediated by duration of IDU (αβ = 0.077, P = 0.03). The association between mental health problems and HCV was partially mediated by duration of IDU and the sequential mediation of duration of IDU and RSS (αβ + αββ = 0.091, P = 0.05).

Conclusions
High HCV rates among young adult PWID in rural New Mexico may be partly explained by mental health problems, duration of IDU and RSS. Mental health services for young adult PWID in rural areas may help decrease HCV transmission in rural areas.
Trial Registration N/A.
Failure and fault classification for smart grids
Authors: Krivohlava Zuzana , Chren Stanislav , Rossi Bruno 
Published: October 2022

AbstractSmart grid (SG) has been designed as a response to the limitations of traditional power grids caused by growing power supply demands. SG is considered a critical infrastructure in which dependability plays a crucial role and manifestation of failures can lead to severe consequences. Architecture-wise, SGs can be decomposed in several layers comprising variety of physical, software, communication and business components, each representing a potential point of failure determined by their underlying faults. In this paper, we present a systematic literature review surveying 30 different faults and failures which can occur in the SG infrastructure. The discovered faults and failures are investigated to extract details about their causes, impacts, detection techniques and counter-measures. Based on the collected information, the faults and failures are classified and mapped to Smart Grid Reference Architecture Model (SGAM), providing a useful frame of reference for practitioners and researchers dealing with hardware and software dependability in this complex domain.
COVID-19’s effects on the Egyptian population’s brain: Could MRI and specialized MR spectroscopic analysis be beneficial?
Authors: Reda Alaa Mohamed , Elsharkawy Ahmed , Dawoud Tamer Mahmoud 
Published: October 2022

Abstract
Background
The recent pandemic of COVID‐19 has thrown the world into chaos due to its high rate of transmissions. Recently viewed neurological manifestations among hospitalized Egyptian patients with COVID‐19 in quarantine centres. Ataxia, disturbed consciousness and convulsions should be further evaluated by MRI and MRS for CNS involvement by SARS‐CoV‐2. How COVID-19 targeting the CNS is still under study, as it is difficult to predict which diagnostic neurological tests will be used to identify high-risk COVID19 patients. MR spectroscopy represents a non-invasive in vivo diagnostic technique for evaluation of metabolic profile of the brain and can reveal important information about the underlying pathologies. Multiple recent reports in the medical literature had confirmed the neurological complications in COVID-19 infection, though few studies has reported the MR spectroscopic findings in these patients. This cross-sectional study aimed to use MRI and MR spectroscopic findings for evaluation of the neurological manifestation of Egyptian COVID‐19 patients.

Results
Ninety-one male and twenty-seven female met the inclusion criteria, with a mean age of 52 years ± 10 (SD) (age range; 12–78 years). The commonest neurological manifestations were disturbed conscious level (82.2%). The most common MRI findings were acute ischemic insult with/without haemorrhagic areas (42.3%), demyelinating patches of altered signal intensity (31.3%). Sixty cases who had haemorrhagic areas were excluded to perform MRS due to contamination of the spectra by blood component. However, the remaining 67 patients had NAA reduction, choline elevation, glutamate/glutamine and lactate elevation in short TE35, with mean of NAA/Cr ratio = 1.04 ± 0.14, Choline/Cr = 0.49 ± 0.04 and Glx/Cr = 1.56 ± 0.22.

Conclusions
During the current pandemic of COVID-19, radiologists should be aware of wide spectrum of MRI and MRS findings of COVID-19-related CNS involvement.
Investigation of the corrosion inhibition potentials of some 2-(4-(substituted)arylidene)-1H-indene-1,3-dione derivatives: density functional theory and molecular dynamics simulation
Authors: Oyeneyin Oluwatoba Emmanuel , Ojo Nathanael Damilare , Ipinloju Nureni , Agbaffa Eric Bamidele , Emmanuel Abiodun Vestor 
Published: October 2022

Abstract
Background
Corrosion is a threat to material strength and durability. Electron-rich organic inhibitor may offer good corrosion mitigation potentials. In this work, anti-corrosion potentials of nine derivatives of 1H-indene-1,3-dione have been investigated using density functional theory (DFT) approach and molecular dynamics (MD) simulation. Chemical reactivity descriptors like energies of lowest unoccupied molecular orbital (ELUMO), highest occupied molecular orbital (EHOMO), electron affinity (A), ionization potential (I), energy gap (ΔEgap), global hardness (η), global softness (σ), electronegativity (χ), electrophilicity (ω), number of transferred electrons (ΔN) and back-donation (ΔEback-donation) were computed at DFT/B3LYP/6-31G(d) theoretical level. The local reactive sites and the charge partitioning on the compounds were studied using Fukui indices and molecular electrostatic potential (MEP) surface analysis. The adsorption behavior and the binding energy of the inhibitors on Fe (110) surface in hydrochloric acid solution were investigated using MD simulation.

Results
The high chemical reactivity, kinetic instability and good corrosion inhibition potentials demonstrated by the inhibitors are rationalized based on their high EHOMO, A, σ, ΔN, ΔEback-donation, and low ΔEgap, ELUMO, I and η. A wide difference of approximately 2.4–3.2 eV between the electronegativities of iron and the 1H-inden-1,3-diones suggests good charge transfer tendency from the latter to the low-lying vacant d-orbitals of iron. The heteroatoms (O and N) and the aromatic moieties are the nucleophilic sites on the inhibitors for effective adsorption on the metal surface as shown by condensed Fukui dual functions and MEP analysis. The MD simulation shows good interaction and strong binding energy between the inhibitor and Fe (110) surface.

Conclusions
Effective surface coverage and displacement of H3O+, Cl− and water molecules from Fe (110) surface by the inhibitors indicate good corrosion inhibition properties of the inden-1,3-diones. 2-((4,7-dimethylnaphthalen-1-yl)methylene)-1H-indene-1,3(2H)-dione display low energy gap, strongest binding interaction and most stabilized iron-inhibitor configuration, hence, the best anti-corrosion potential.

Graphical abstract
Production of biodiesel from waste cooking oil using mesoporous MgO-SnO2 nanocomposite
Authors: Velmurugan Amirthavalli , Warrier Anita R. 
Published: October 2022

AbstractMesoporous, bifunctional MgO-SnO2 nanocatalysts with enhanced surface area are used for producing biodiesel from waste cooking oil. Biodiesel with yield of 80% is achieved within the first 20 min when transesterification is carried out at an optimum condition of 18:1 methanol to oil ratio, 2 wt% of nanocatalyst, and at a reaction temperature of 60 °C. The conversion gives a maximum yield of 88% when transesterification is allowed to continue for 120 min. The waste cooking oil used in this work is dominated with linoleic acid and oleic acid, which during transesterification gets converted into methyl linoleate and 9-octadecenoic acid methyl ester. These nanocatalysts are fabricated using a composite of rutile (tetragonal) phase SnO2 and cubic phase MgO nanostructures with prominent crystal orientation along [211] and [200] plane respectively. The MgO-SnO2 nanocomposites with an enhanced surface area of 31 m2/g, basic sites of 2 mmol/g, and particle size of ~15 nm are synthesized by novel sequential thermal decomposition and sol-gel technique. The synthesized wide band gap nanocomposites have Mg and Sn in the ratio of 15:1 and do not have any impurity phases as observed in the X-ray diffraction pattern and EDS spectrum. The presence of surface oxygen states and Mg2+ and Sn4+ oxidative states is responsible for the catalytic activity and recyclability displayed by the composites. This work signifies the role of nanocomposites and their synthesis conditions in improving the rate of transesterification. These metal oxide nanocomposites which are nontoxic, stable, cost effective, and easier to synthesis are promising catalysts for large-scale transesterification of waste cooking oil to biodiesel.
Community Member and Health Care Provider Perspectives on Communication With Chinese American Patients With Cancer: A Qualitative Study
Authors: Robbel Courtney , Vesel Linda , Ernst Emma , He Yang , Vesel Tamara 
Published: October 2022

PURPOSE: Effective health care provider-patient discussions of cancer diagnoses and prognoses are essential to enhance health outcomes in oncology. Chinese Americans have been designated an underserved population in oncology by the National Institutes of Health. We explored the perspectives of Boston Chinese American community members and health care providers regarding communication preferences and decision making in oncology care. METHODS: In this inductive, qualitative study, we conducted virtual, semi-structured interviews with 21 health care providers with experience in caring for Chinese American patients with cancer and 25 older Boston Chinatown community members who self-identify as Chinese or Chinese American. Thematic analysis was performed. Results: Two major themes were identified. First, provider communication should incorporate a gentle but truthful approach, careful word choice, authority-led style, and professionalism. Second, the family plays a large role in medical and nonmedical settings, and a family-centered approach to communication should be used. Conclusion: Our study builds on and challenges current knowledge regarding oncologic communication with Chinese American patients. A focus on the improvement of provider-patient cancer communication for Chinese Americans will improve care quality and satisfaction among patients and clinicians alike and serve to decrease disparities in care.
Molecular Characterization of Circulating Tumor DNA in Pediatric Rhabdomyosarcoma: A Feasibility Study
Authors: Ruhen Olivia , Lak Nathalie S.M. , Stutterheim Janine , Danielli Sara G. , Chicard Mathieu , Iddir Yasmine , Saint-Charles Alexandra , Di Paolo Virginia , Tombolan Lucia , Gatz Susanne A. , Aladowicz Ewa , Proszek Paula , Jamal Sabri , Stankunaite Reda , Hughes Deborah , Carter Paul , Izquierdo Elisa , Wasti Ajla , Chisholm Julia C. , George Sally L. , Pace Erika , Chesler Louis , Aerts Isabelle , Pierron Gaelle , Zaidi Sakina , Delattre Olivier , Surdez Didier , Kelsey Anna , Hubank Michael , Bonvini Paolo , Bisogno Gianni , Di Giannatale Angela , Schleiermacher Gudrun , Schäfer Beat W. , Tytgat Godelieve A.M. , Shipley Janet 
Published: October 2022

PURPOSERhabdomyosarcomas (RMS) are rare neoplasms affecting children and young adults. Efforts to improve patient survival have been undermined by a lack of suitable disease markers. Plasma circulating tumor DNA (ctDNA) has shown promise as a potential minimally invasive biomarker and monitoring tool in other cancers; however, it remains underexplored in RMS. We aimed to determine the feasibility of identifying and quantifying ctDNA in plasma as a marker of disease burden and/or treatment response using blood samples from RMS mouse models and patients.METHODSWe established mouse models of RMS and applied quantitative polymerase chain reaction (PCR) and droplet digital PCR (ddPCR) to detect ctDNA within the mouse plasma. Potential driver mutations, copy-number alterations, and DNA breakpoints associated with PAX3/ 7-FOXO1 gene fusions were identified in the RMS samples collected at diagnosis. Patient-matched plasma samples collected from 28 patients with RMS before, during, and after treatment were analyzed for the presence of ctDNA via ddPCR, panel sequencing, and/or whole-exome sequencing.RESULTSHuman tumor-derived DNA was detectable in plasma samples from mouse models of RMS and correlated with tumor burden. In patients, ctDNA was detected in 14/18 pretreatment plasma samples with ddPCR and 7/7 cases assessed by sequencing. Levels of ctDNA at diagnosis were significantly higher in patients with unfavorable tumor sites, positive nodal status, and metastasis. In patients with serial plasma samples (n = 18), fluctuations in ctDNA levels corresponded to treatment response.CONCLUSIONComprehensive ctDNA analysis combining high sensitivity and throughput can identify key molecular drivers in RMS models and patients, suggesting potential as a minimally invasive biomarker. Preclinical assessment of treatments using mouse models and further patient testing through prospective clinical trials are now warranted.