bluuebunny/crossref_metadata_2025_split huggingface dataset:
REDCap mobile data collection: Using implementation science to explore the potential and pitfalls of a digital health tool in routine voluntary medical male circumcision outreach settings in Zimbabwe
Authors: Tran Vi , Gwenzi Farai , Marongwe Phiona , Rutsito Olbarn , Chatikobo Pesanai , Murenje Vernon , Hove Joseph , Munyaradzi Tinashe , Rogers Zoe , Tshimanga Mufuta , Sidile-Chitimbire Vuyelwa , Xaba Sinokuthemba , Ncube Gertrude , Masimba Lewis , Makunike-Chikwinya Batsirai , Holec Marrianne , Barnhart Scott , Weiner Bryan , Feldacker Caryl 
Published: July 2022

Background Digital data collection tools improve data quality but are limited by connectivity. ZAZIC, a Zimbabwean consortium focused on scaling up male circumcision (MC) services, provides MC in outreach settings where both data quality and connectivity is poor. ZAZIC implemented REDCap Mobile app for data collection among roving ZAZIC MC nurses. To inform continued scale-up or discontinuation, this paper details if, how, and for whom REDCap improved data quality using the Reach, Effectiveness, Adoption, Implementation, and Maintenance (RE-AIM) framework. Methods Data were collected for this retrospective, cross-sectional study for nine months, from July 2019 to March 2020, before COVID-19 paused MC services. Data completeness was compared between paper- and REDCap-based tools and between two ZAZIC partners using two sample, one-tailed t-tests. Results REDCap reached all roving nurses who reported 26,904 MCs from 1773 submissions. REDCap effectiveness, as measured by data completeness, decreased from 89.2% in paper to 76.6% in REDCap app for Partner 1 ( p < 0.001, 95% CI: −0.24, −0.12) but increased modestly from 86.2% to 90.3% in REDCap for Partner 2 ( p = 0.05, 95% CI: -.007, 0.12). Adoption of REDCap was 100%; paper-based reporting concluded in October 2019. Implementation varied by partner and user. Maintenance appeared high. Conclusion Although initial transition from paper to REDCap showed mixed effectiveness, post-hoc analysis from service resumption found increased REDCap data completeness across partners, suggesting locally-led momentum for REDCap-based data collection. Staff training, consistent mentoring, and continued technical support appear critical for continued use of digital health tools for quality data collection in rural Zimbabwe and similar low connectivity settings.
Gender-Related Differences in Social Participation Among Japanese Elderly Individuals During the COVID-19 Pandemic: A Cross-Sectional Survey
Authors: Goto Ryohei , Ozone Sachiko , Kawada Shogo , Yokoya Shoji 
Published: July 2022

Introduction/Objectives: The health of elderly individuals is known to benefit from maintaining societal involvement and relationships with other people, such as through social participation. We aimed to determine trends in the percentage of Japanese elderly people who engaged in social participation before and during the coronavirus disease 2019 (COVID-19) pandemic in one municipality in Japan, and compared differences in this status by gender. Methods: We conducted a cross-sectional questionnaire survey. Questionnaires were sent by mail to 3000 people aged 65 to 85 years who were randomly selected by the administrative staff of the city. Participant characteristics (age, gender, working status, residential status) and their economic status, daily physical activity, and social participation status were obtained at 3 time points: (1) before the COVID-19 pandemic in January 2020; (2) immediately prior to the declaration of a state of emergency in April 2020; and (3) in January 2021, 1 year after (1). Results: A total of 1301 people responded to the survey. The mean age was 73.3 (SD 5.5) years, and 690 (53.0%) were women. There were significant gender differences in terms of living alone, employment status, and amount of physical activity. The number of people reporting social participation gradually decreased from 543 respondents (41.7%) at (1) to 319 (24.5%) at (2) and 251 (19.3%) at (3). Women were more likely to demonstrate reduced social participation. Conclusions: Elderly individuals, particularly women, reported decreased social participation during the pandemic.
Histo-Molecular Profile of Breast Cancer in Young Women in Togo
Authors: Djiwa Toukilnan , Koui Baumaney , Simgban Panakinao , Mézéwè Sama Bagassam , Bombonne Mayi , Doukouré Brahima , Darré Tchin 
Published: July 2022

Introduction: The frequency of breast cancer in young women, corresponding to women under 40 years of age; varies from 5% to 7% in developed countries. The objective of this study was to contribute to the improvement of the management of breast cancer in young women in Togo, by establishing the molecular classification of these cancers. Methodology: This was a retrospective descriptive and analytical study from January 2010 to December 2020. Results: About 35 cases of breast cancer were identified in women under 40 years of age. The average age was 35.4 ± 3.5 years. The right breast was affected in 18 cases (51.43%). Histologically, 30 cases (85.72%) were invasive carcinoma of no special type (NST), 2 cases (5.71%) were invasive lobular carcinoma and micro-papillary carcinoma respectively, and 1 case (2.86%) was tubular carcinoma. There were 6 cases (17.1%) of grade I, 25 cases (71.4%) of grade II, and 4 cases (11.4%) of grade III. Molecularly, there were 20 cases (57.1%) of triple-negative subtype, 6 cases (17.1%) of Luminal B subtype, 05 cases (14.3%) of HER2-enriched subtype and 4 cases (11.4%) of Luminal A subtype. Conclusion: Breast cancers of young Togolese women express very weakly hormone receptors, with a predominance of a triple negative subtype.
Arthroscopic Treatment of Anterior Shoulder Instability Using Suture Anchors With Pre-Tied Knots in the Beach Chair Position
Authors: Thomson Cameron G. , Sabbagh Ramsey S. , Shah Nihar S. , Figueras Jorge H. , Grawe Brian M. 
Published: July 2022

Background: Arthroscopic Bankart repair is routinely performed for treatment of anterior shoulder instability. Although an overall successful procedure, arthroscopic free-hand knot tying can be challenging and inconsistent, even for experienced surgeons. Knotless anchors, on the other hand, pose challenges in developing consistent good loop security prior to implantation, and, in the absence of a secure loop tissue tension on the glenoid face, might be inadequate. As an alternative, suture anchors with pre-tied knots offer the strength and security of knots without the need to perform complex and unreliable free-handed ties. Furthermore, loop security is consistent, reliable, and effective with each anchor. Indications: Patients with anterior labral tears and minimal bone loss are candidates for arthroscopic Bankart repair. The goal of surgery is to restore a robust tissue profile and statically keep the humeral head well-seated within the glenohumeral joint space. Technique Description: We demonstrate how to perform Bankart repair using suture anchors with pre-tied knots. The torn labrum is first mobilized and elevated, and suture anchors are placed along the glenoid rim from inferior to superior, treating the torn tissue as “rungs of a ladder.” The pre-tied sliding knot secures the restored labrum, along with glenohumeral ligaments and capsule, as necessary, using only a limited number of incrementally tensioned half hitch knots. Results: Of the 30 patients treated for anterior shoulder instability using this technique, the majority met the minimal clinically important improvement in the American Shoulder and Elbow Surgeons Shoulder Score, Western Ontario Shoulder Instability Index, and Marx activity scale by 2 years. Two patients reported experiencing subsequent minor subluxation events that improved following short courses of physical therapy, and no patients sustained subsequent frank dislocations. Discussion/Conclusion: Using suture anchors with pre-tied knots for arthroscopic Bankart repair allows for fast, easy, and consistently dependable reconstruction of the labrum and leads to reliable clinical outcomes.
Grab regulates transferrin receptor recycling and iron uptake in developing erythroblasts
Authors: Chen Mengying , Zhang Yuhan , Jiang Kailun , Wang Weixi , Feng He , Zhen Ru , Moo Chingyee , Zhang Zhuonan , Shi Jiahai , Chen Caiyong 
Published: July 2022

Abstract
Developing erythroblasts acquire massive amounts of iron through the transferrin (Tf) cycle, which involves endocytosis, sorting, and recycling of the Tf-Tf receptor (Tfrc) complex. Previous studies on the hemoglobin-deficit (hbd) mouse have shown that the exocyst complex is indispensable for the Tfrc recycling; however, the precise mechanism underlying the efficient exocytosis and recycling of Tfrc in erythroblasts remains unclear. Here, we identify the guanine nucleotide exchange factor Grab as a critical regulator of the Tf cycle and iron metabolism during erythropoiesis. Grab is highly expressed in differentiating erythroblasts. Loss of Grab diminishes the Tfrc recycling and iron uptake, leading to hemoglobinization defects in mouse primary erythroblasts, mammalian erythroleukemia cells, and zebrafish embryos. These defects can be alleviated by supplementing iron together with hinokitiol, a small-molecule natural compound that can mediate iron transport independent of the Tf cycle. Mechanistically, Grab regulates the exocytosis of Tfrc-associated vesicles by activating the GTPase Rab8, which subsequently promotes the recruitment of the exocyst complex and vesicle exocytosis. Our results reveal a critical role for Grab in regulating the Tf cycle and provide new insights into iron homeostasis and erythropoiesis.
Combining single-cell tracking and omics improves blood stem cell fate regulator identification
Authors: Wehling A. , Loeffler D. , Zhang Y. , Kull T. , Donato C. , Szczerba B. , Camargo Ortega G. , Lee M. , Moor A. , Göttgens B. , Aceto N. , Schroeder T. 
Published: July 2022

AbstractMolecular programs initiating cell fate divergence (CFD) are difficult to identify. Current approaches usually compare cells long after CFD initiation, therefore missing molecular changes at its start. Ideally, single cells that differ in their CFD molecular program but are otherwise identical are compared early in CFD. This is possible in diverging sister cells, which were identical until their mother’s division and thus differ mainly in CFD properties. In asymmetrically dividing cells, divergent daughter fates are prospectively committed during division, and diverging sisters can thus be identified at the start of CFD. Using asymmetrically dividing blood stem cells, we developed a pipeline (ie, trackSeq) for imaging, tracking, isolating, and transcriptome sequencing of single cells. Their identities, kinship, and histories are maintained throughout, massively improving molecular noise filtering and candidate identification. In addition to many identified blood stem CFD regulators, we offer here this pipeline for use in CFDs other than asymmetric division.
Efflux capacity and aldehyde dehydrogenase both contribute to CD8+ T-cell resistance to posttransplant cyclophosphamide
Authors: Patterson Michael T. , Nunes Natalia S. , Wachsmuth Lucas P. , Panjabi Amrit , Fletcher Rochelle E. , Khan Shanzay M. , Dimitrova Dimana , Kanakry Jennifer A. , Luznik Leo , Kanakry Christopher G. 
Published: July 2022

Abstract
Mechanisms of T-cell survival after cytotoxic chemotherapy, including posttransplantation cyclophosphamide (PTCy), are not well understood. Here, we explored the impact of PTCy on human CD8+ T-cell survival and reconstitution, including what cellular pathways drive PTCy resistance. In major histocompatibility complex (MHC)-mismatched mixed lymphocyte culture (MLC), treatment with mafosfamide, an in vitro active cyclophosphamide analog, preserved a relatively normal distribution of naïve and memory CD8+ T cells, whereas the percentages of mucosal-associated invariant T (MAIT) cells and phenotypically stem cell memory (Tscm) T-cell subsets were increased. Activated (CD25+) and proliferating CD8+ T cells were derived from both naïve and memory subsets and were reduced but still present after mafosfamide. By contrast, cyclosporine-A (CsA) or rapamycin treatment preferentially maintained nonproliferating CD25− naïve cells. Drug efflux capacity and aldehyde dehydrogenase-1A1 expression were increased in CD8+ T cells in allogeneic reactions in vitro and in patients, were modulated by common γ-chain cytokines and the proliferative state of the cell, and contributed to CD8+ T-cell survival after mafosfamide. The CD8+ T-cell composition early after hematopoietic cell transplantation (HCT) in PTCy-treated patients was dominated by CD25+ and phenotypically memory, including Tscm and MAIT, cells, consistent with MLC. Yet, MHC-mismatched murine HCT studies revealed that peripherally expanded, phenotypically memory T cells 1 to 3 months after transplant originated largely from naïve-derived rather than memory-derived T cells surviving PTCy, suggesting that initial resistance and subsequent immune reconstitution are distinct. These studies provide insight into the complex immune mechanisms active in CD8+ T-cell survival, differentiation, and reconstitution after cyclophosphamide, with relevance for post-HCT immune recovery, chemotherapy use in autologous settings, and adoptive cellular therapies.
Venetoclax enhances the efficacy of therapeutic antibodies in B-cell malignancies by augmenting tumor cell phagocytosis
Authors: Vogiatzi Fotini , Heymann Julia , Müller Kristina , Winterberg Dorothee , Drakul Aneta , Rösner Thies , Lenk Lennart , Heib Michelle , Gehlert Carina Lynn , Cario Gunnar , Schrappe Martin , Claviez Alexander , Bornhauser Beat , Bourquin Jean-Pierre , Bomken Simon , Adam Dieter , Frielitz Fabian-Simon , Maecker-Kolhoff Britta , Stanulla Martin , Valerius Thomas , Peipp Matthias , Kellner Christian , Schewe Denis M. 
Published: July 2022

Abstract
Immunotherapy has evolved as a powerful tool for the treatment of B-cell malignancies, and patient outcomes have improved by combining therapeutic antibodies with conventional chemotherapy. Overexpression of antiapoptotic B-cell lymphoma 2 (Bcl-2) is associated with a poor prognosis, and increased levels have been described in patients with “double-hit” diffuse large B-cell lymphoma, a subgroup of Burkitt’s lymphoma, and patients with pediatric acute lymphoblastic leukemia harboring a t(17;19) translocation. Here, we show that the addition of venetoclax (VEN), a specific Bcl-2 inhibitor, potently enhanced the efficacy of the therapeutic anti-CD20 antibody rituximab, anti-CD38 daratumumab, and anti-CD19-DE, a proprietary version of tafasitamab. This was because of an increase in antibody-dependent cellular phagocytosis by macrophages as shown in vitro and in vivo in cell lines and patient-derived xenograft models. Mechanistically, double-hit lymphoma cells subjected to VEN triggered phagocytosis in an apoptosis-independent manner. Our study identifies the combination of VEN and therapeutic antibodies as a promising novel strategy for the treatment of B-cell malignancies.
Copy number variation at the 22q11.2 locus influences prevalence, severity, and psychiatric impact of sleep disturbance
Authors: O’Hora Kathleen P. , Lin Amy , Kushan-Wells Leila , Bearden Carrie E. 
Published: July 2022

Abstract
Background
Sleep disturbance is common, impairing, and may affect symptomatology in developmental neuropsychiatric disorders. Here, we take a genetics-first approach to study the complex role of sleep in psychopathology. Specifically, we examine severity of sleep disturbance in individuals with a reciprocal copy number variant (CNV) at the 22q11.2 locus and determine sleep’s effect on psychiatric symptoms. CNVs (deletion or duplication) at this locus confer some of the greatest known risks of neuropsychiatric disorders; recent studies suggest the 22q11.2 deletion negatively impacts sleep, but sleep disruption associated with 22q11.2 duplication has not been investigated.

Methods
We compared subjective sleep disturbance and its relationship to psychiatric symptoms cross-sectionally and longitudinally over 1 year in 107 22q11.2 deletion (22qDel) carriers (14.56±8.0 years; 50% male), 42 22q11.2 duplication (22qDup) carriers (16.26±13.1 years; 54.8% male), and 88 age- and sex-matched controls (14.65±7.4 years; 47.1% male). Linear mixed models were used to compare sleep disturbance, assessed via the Structured Interview for Psychosis-Risk Syndromes (SIPS), across groups. Next, CNV carriers were categorized as good or poor sleepers to investigate sleep effects on multiple neurobehavioral traits: psychosis-risk symptoms (SIPS), autism-related behaviors (Repetitive Behavior Scale (RBS) and Social Responsiveness Scale (SRS)), real-world executive function (Behavior Rating Inventory of Executive Function (BRIEF)), and emotional/behavioral problems (Child Behavior Checklist (CBCL)). Linear mixed models tested the effect of sleep category and a group-by-sleep interaction on each measure, cross-sectionally and longitudinally.

Results
22qDel and 22qDup carriers both reported poorer sleep than controls, but did not differ from each other. Cross-sectionally and longitudinally, poor sleepers scored higher on positive symptoms, anxious/depressed, somatic complaints, thought problems, and aggressive behavior, as well as RBS and SRS total scores. There were significant group-by-sleep interactions for positive symptoms and the majority of CBCL subdomains, in which the difference between good and poor sleepers was larger in 22qDel compared to 22qDup.

Conclusions
Our findings indicate that CNVs at the 22q11.2 locus impact sleep which, in turn, influences psychopathology. Sleep disturbances can differentially impact psychopathology, depending on 22q11.2 gene dosage. Our findings serve as a starting point for exploring a genetic basis for sleep disturbance in developmental neuropsychiatric disorders.
Assessing robustness in multimodal transportation systems: a case study in Lisbon
Authors: Aparicio Joao Tiago , Arsenio Elisabete , Henriques Rui 
Published: July 2022

Abstract
Introduction
Worldwide public transport systems are exposed to disruptions caused by malfunctions, accidents, maintenance, reduced fleet, and disasters, compromising mobility. Transport networks’ multimodal planning and management can be explored to increase their robustness against these events. In this context, this research paper proposes and empirically compares methods to assess the robustness of a multimodal transport network, looking at aspects regarding the single-mode and multimodal network topology.

Materials and Methods
We hypothesize that the appropriate multilayered and traffic sensitive modeling of a multimodal transport network can help characterize robustness and further unravel vulnerabilities related to the integration of different transport modes. Using metric-based targeting, we evaluate how the network decreases performance when simulating failures on stations and pathways using different scenarios. The following six extraction strategies for nodes and edges were used in the simulation: Random removal; Initial Degree removal; Initial Betweenness removal; Recalculate Degree removal; Recalculate Betweenness removal; and Multimodal Hubs removal. Lisbon’s public transport is used as a case study and is modeled as a multiplex network integrating eight different modes of transport. Proposing a novel normalized version of assessing the impact of failures, we were able to compare side by side the robustness of each modality layer, regardless of their size. Lastly, we simulate cascading events such as the breakdown of an entire transportation line.

Conclusions
Using different ways to induce failures in the network, we observe that to leave all nodes completely disconnected, we would need to remove about half the network nodes, highlighting the robustness of the Lisbon public transport network. Comparing different failure scenarios, methods that rely on recalculating network metrics yield a higher impact on the network robustness assessment. The impact of different events is quantified, showing that failures in stations are generally more dangerous than in pathways and offering views on the consequences of deactivating particular network modules. Overall, the results of this study allow decision-makers to gain further understanding of the topological vulnerabilities of a transportation network.
MTAGCN: predicting miRNA-target associations in Camellia sinensis var. assamica through graph convolution neural network
Authors: Feng Haisong , Xiang Ying , Wang Xiaosong , Xue Wei , Yue Zhenyu 
Published: July 2022

AbstractBackgroundMircoRNAs (miRNAs) play a central role in diverse biological processes ofCamellia sinensisvar.assamica (CSA) through their associations with target mRNAs, including CSA growth, development and stress response. However, although the experiment methods of CSA miRNA-target identifications are costly and time-consuming, few computational methods have been developed to tackle the CSA miRNA-target association prediction problem.ResultsIn this paper, we constructed a heterogeneous network for CSA miRNA and targets by integrating rich biological information, including a miRNA similarity network, a target similarity network, and a miRNA-target association network. We then proposed a deep learning framework of graph convolution networks with layer attention mechanism, named MTAGCN. In particular, MTAGCN uses the attention mechanism to combine embeddings of multiple graph convolution layers, employing the integrated embedding to score the unobserved CSA miRNA-target associations.DiscussionComprehensive experiment results on two tasks (balanced task and unbalanced task) demonstrated that our proposed model achieved better performance than the classic machine learning and existing graph convolution network-based methods. The analysis of these results could offer valuable information for understanding complex CSA miRNA-target association mechanisms and would make a contribution to precision plant breeding.
Identification and characterization of transposable element AhMITE1 in the genomes of cultivated and two wild peanuts
Authors: Tang Yanyan , Li Xiaoting , Hu Changli , Qiu Xiaochen , Li Jingjing , Li Xin , Zhu Hong , Wang Jingshan , Sui Jiongming , Qiao Lixian 
Published: July 2022

Abstract
Background
The cultivated peanut (Arachis hypogaea L., AABB) is an allotetraploid hybrid between two diploid peanuts, A. duranensis (AA genome) and A. ipaensis (BB genome). Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons with high copy numbers, play important roles in genome evolution and diversification. AhMITE1, a member of the MITE family of transposons, but information on the peanut genomes is still limited. Here, we analyzed AhMITE1, AuMITE1 and ApMITE1 in the cultivated (A. hypogaea) and two wild peanut (A. duranensis and A. ipaensis) genomes.

Results
The cultivated and the two wild peanut genomes harbored 142, 14 and 21 AhMITE1, AuMITE1 and ApMITE1 family members, respectively. These three family members exhibited highly conserved TIR sequences, and insertions preferentially occurred within 2 kb upstream and downstream of gene-coding and AT-rich regions. Phylogenetic and pairwise nucleotide diversity analysis showed that AhMITE1 and ApMITE1 family members have undergone one round of amplification bursts during the evolution of the peanut genome. PCR analyses were performed in 23 peanut varieties and demonstrated that AhMITE1 is an active transposon and that hybridization or chemical mutagenesis can promote the mobilization of AhMITE1.

Conclusions
AhMITE1, AuMITE1 and ApMITE1 family members were identified based on local BLAST search with MAK between the cultivated and the two wild peanut genomes. The phylogenetic, nucleotide diversity and variation copy numbers of AhMITE1, AuMITE1 and ApMITE1 members provides opportunities for investigating their roles during peanut evolution. These findings will contribute to knowledge on diversity of AhMITE1, provide information about the potential impact on the gene expression and promote the development of DNA markers in peanut.