bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of heterogeneous environmental regulation policies on the strategy of pollutant discharge for enterprise: an evolutionary game approach
Authors: Zhou Zhiqiang , Feng Huanhuan , Wang Huilin , Wang Kexi 
Published: August 2022

Abstract
To improve the effectiveness of the government’s environmental regulation policy, this study aims to solve the problem of the low willingness of enterprises to cooperate. This study redefined and defined the types of environmental regulations based on the policy realization path, and gradually added them into the game system between the government and enterprises, using the evolutionary game to analyze the impact of different regulatory means on the enterprises’ choice of whether or not to cooperate with the regulatory policy, and using MATLAB to perform a numerical simulation to compare the degree of impact. In this way, the best regulatory objects of different regulatory means were determined, and the theoretical basis and practical reference for the government to implement classified governance policies were provided. In addition, this study for the first-time classified enterprises based on the relationship between the number of pollutants discharged by enterprises and the government’s pollutant discharge quota, and incorporated them into the model for further research. The results showed that the government should implement administrative regulations for enterprises discharging a small number of pollutants; implement a combination of administrative and market-oriented regulations for enterprises discharging a medium number of pollutants; implement administrative, market-oriented, and socialized regulations at the same time for enterprises discharging a large number of pollutants. Moreover, these should be based on the premise of reasonably arranging the performance appraisal of local governments.
Assessment of Physical Work Demands of Home Care Workers in Norway: An Observational Study Using Wearable Sensor Technology
Authors: Tjøsvoll Svein O , Wiggen Øystein , Gonzalez Victor , Seeberg Trine M , Elez Redzovic Skender , Frostad Liaset Ingeborg , Holtermann Andreas , Steiro Fimland Marius 
Published: August 2022

Abstract

Objectives
High physical work demands are believed to be partly responsible for the high sickness absence among home care workers, but no studies have assessed their physical work demands using precise device-based measurements. Hence, the objective of this observational study was to assess physical work demands in home care, using wearable sensors.

Methods
From six home care units in a large municipality in Norway, 114 of 195 eligible home care workers filled in a questionnaire, a diary about work hours, and wore five accelerometers, and a heart rate sensor for up to six consecutive workdays.

Results
On average, the homecare workers spent 50% of the working hours sitting, 25.2% standing, 11.4% moving, 8.3% walking fast, 1.9% walking slow, 1.2% stair-climbing, 0.3% cycling, and 0.05% running. We found the following exposures to demanding postures: arm-elevation in an upright body position ≥30° was 36.7%, ≥60° was 4.1%, and ≥90°was 0.5%; forward trunk inclination in an upright body position ≥30° was 9.9%, ≥60° was 4%, and ≥90° was 1%; and for kneeling it was 0.8%. We found the average cardiovascular load (%heart rate reserve) during work to be 28%. There was considerable individual variation in these physical exposures at work.

Conclusions
This study presents precise information on various physical work demands of home care workers in Norway. Home care workers spent on average half the workday sitting and the remaining time in various occupational physical activities. Presently, few device-based exposure limits have been proposed for acceptable amounts of occupational physical exposures, but the level of arm-elevation, forward trunk inclination, and the considerable variation of physical workloads among home care workers, indicate that preventive measures should be taken.
Characteristics and Treatment of Patients Diagnosed With Paradoxical Adipose Hyperplasia After Cryolipolysis: A Case Series and Scoping Review
Authors: Cox Elizabeth A , Nichols D Spencer , Riklan Joshua E , Pomputius Ariel , Mehta Sonia D , Mast Bruce A , Furnas Heather , Canales Francisco , Sorice-Virk Sarah 
Published: August 2022

Abstract

Background
Paradoxical adipose hyperplasia (PAH), a rare side effect of CoolSculpting (cryolipolysis), is characterized by fatty enlargement of the treatment area occurring months after the procedure.

Objectives
The purpose of this study was to report a retrospective case series of patients diagnosed with PAH at the authors’ institution, increase the collective understanding of this complication and subsequent management, and raise the question of who should ethically perform cryolipolysis.

Methods
All participants diagnosed with PAH by a plastic surgeon at a large academic medical center were identified. Demographic information, medical history, procedure details, time to PAH diagnosis, and corrective surgical intervention details were collected. Mean duration of time from cryolipolysis treatment to diagnosis of PAH was calculated, along with other descriptive statistics. A scoping review of all PAH literature published in PubMed, Embase, and Web of Science was also conducted.

Results
Four patients diagnosed with PAH after cryolipolysis were identified for inclusion in this study. The calculated incidence of PAH at our center was 0.67%. All patients requested therapy for PAH and subsequently underwent either liposuction, abdominoplasty, or both. The mean duration of in-person follow-up time after final surgical treatment of PAH was 13.8 + 19.8 months (range, 2.8-43.5). Fortunately, no patients showed signs of PAH recurrence, and 3 out of 4 patients did not show signs of residual deformity.

Conclusions
Findings from this patient cohort and scoping review provide evidence that although revisions may be required, conventional body contouring methods, not in the armamentarium of non-plastic surgeon practitioners, effectively alleviated PAH.

Level of Evidence: 4
Patient Satisfaction, Recommendation Rate, and Patient Comfort With an FDA-Cleared Cryolipolysis System
Authors: Altmann Jens , Jehle Felix , Mang Werner 
Published: August 2022

Abstract

Background
Cryolipolysis is a non-surgical procedure for subcutaneous fat layer reduction by controlled cooling. During the past few years, the use of cryolipolysis for non-invasive body contouring has increased significantly.

Objectives
This retrospective study examines patient satisfaction, recommendation rate and patient comfort with the use of an FDA-cleared system (CoolSculpting Elite, Allergan Aesthetics, AbbVie Company, Irvine CA) for cryolipolysis at a single clinic and reports on the results.

Methods
Between December 2020 and January 2022, 91 patients were treated with an FDA-cleared cryolipolysis system. To assess patient satisfaction, patients were asked to complete clinical questionnaires three months after their last treatment session. The following questions were asked: painfulness of the treatment, complications, satisfaction with the treatment, consideration of further treatment sessions, and willingness to further recommend the treatment.

Results
Eighty-four percent of the 91 patients were female, and 16% were male. The average age was 45.5 years and the mean BMI was 26 kg/m2. Patients rated the treatment in terms of pain and discomfort experienced during the procedures on a scale of 1 to 5, with the value 1 representing not painful and 5 as extremely painful. 40% of the patients evaluated the procedure with 1, 38% with 2, 19% with 3, 1% with 4 and 2% with 5. With respect to satisfaction rates, 66% rated the treatment on a scale from 1 to 5 with 1, 18% with 2, 7% with 3, 7% with 4 and 3% with 5, with 1 indicating very satisfied and 5 indicating very dissatisfied. Thus, the overall level of satisfaction (the sum of scale values 1 and 2) amounts to 84%. Of 91 patients, 88% would agree to further treatment and 92% would recommend the therapy to others. All patients reported temporary tissue reactions such as swelling and redness which did not require any further treatment and were self-limiting. Serious or permanent complications did not occur.

Conclusions
The results of our study show that cryolipolysis is a safe and effective method for non-surgical body contouring, providing a high degree of patient satisfaction and recommendation rate.

Level of Evidence: 4
Regulatory T-cell therapy approaches
Authors: McCallion Oliver , Bilici Merve , Hester Joanna , Issa Fadi 
Published: August 2022

SummaryRegulatory T cells (Tregs) have enormous therapeutic potential to treat a variety of immunopathologies characterized by aberrant immune activation. Adoptive transfer of ex vivo expanded autologous Tregs continues to progress through mid- to late-phase clinical trials in several disease spaces and has generated promising preliminary safety and efficacy signals to date. However, the practicalities of this strategy outside of the clinical trial setting remain challenging. Here, we review the current landscape of regulatory T-cell therapy, considering emergent approaches and technologies presenting novel ways to engage Tregs, and reflect on the progress necessary to deliver their therapeutic potential to patients.
B cell contribution to immunometabolic dysfunction and impaired immune responses in obesity
Authors: Oleinika Kristine , Slisere Baiba , Catalán Diego , Rosser Elizabeth C 
Published: August 2022

SummaryObesity increases the risk of type 2 diabetes mellitus, cardiovascular disease, fatty liver disease, and cancer. It is also linked with more severe complications from infections, including COVID-19, and poor vaccine responses. Chronic, low-grade inflammation and associated immune perturbations play an important role in determining morbidity in people living with obesity. The contribution of B cells to immune dysregulation and meta-inflammation associated with obesity has been documented by studies over the past decade. With a focus on human studies, here we consolidate the observations demonstrating that there is altered B cell subset composition, differentiation, and function both systemically and in the adipose tissue of individuals living with obesity. Finally, we discuss the potential factors that drive B cell dysfunction in obesity and propose a model by which altered B cell subset composition in obesity underlies dysfunctional B cell responses to novel pathogens.
Optimization of Timing of Maternal Pertussis Immunization From 6 Years of Postimplementation Surveillance Data in England
Authors: Amirthalingam Gayatri , Campbell Helen , Ribeiro Sonia , Stowe Julia , Tessier Elise , Litt David , Fry Norman K , Andrews Nick 
Published: August 2022

Abstract

Background
England’s third-trimester maternal pertussis vaccination, introduced in October 2012, was extended to the second trimester in 2016. Maternal vaccination provides high protection against infant disease, but routine second-trimester vaccination has not previously been assessed.

Methods
National laboratory-confirmed pertussis case surveillance determined vaccination history, maternal vaccination history and hospitalization. Pertussis hospital admissions between 2012 and 2019 were extracted from the Hospital Episode Statistics data set. Vaccine effectiveness (VE) was calculated for pertussis case patients born between October 2012 and September 2018 using the screening method and matching with a nationally representative data set.

Results
Higher coverage was observed after earlier maternal vaccination with approximately 40% of pregnant women vaccinated ≥13 weeks before delivery. Cases and hospitalizations stabilized at low levels in younger infants but remained elevated in older infants, children, and adults. No deaths occurred in infants with vaccinated mothers after 2016. Of 1162 laboratory-confirmed pertussis cases in the study, 599 (52%) were in infants aged &lt;93 days: 463 (77%) with unvaccinated and 136 (23%) with vaccinated mothers. The VE was equivalent in infants with mothers vaccinated at different gestational periods except in those with mothers vaccinated between 7 days before and 41 days after delivery. Children whose mothers were unvaccinated but with vaccination in a previous pregnancy had a VE against disease of 44% (95% confidence interval, 19%–75%). There was no increased disease risk after primary vaccination in children with mothers vaccinated at least 7 days before delivery.

Conclusions
National policy recommending vaccination in the second trimester increased earlier maternal vaccine uptake with sustained high VE and impact against early infant disease.
Left-atrial long-axis shortening allows effective quantification of atrial function and optimized risk prediction following acute myocardial infarction
Authors: Backhaus Sören J , Rösel Simon F , Stiermaier Thomas , Schmidt-Rimpler Jonas , Evertz Ruben , Schulz Alexander , Lange Torben , Kowallick Johannes T , Kutty Shelby , Bigalke Boris , Gutberlet Matthias , Hasenfuß Gerd , Thiele Holger , Eitel Ingo , Schuster Andreas 
Published: August 2022

Abstract

Aims
Deformation imaging enables optimized risk prediction following acute myocardial infarction (AMI). However, costly and time-consuming post processing has hindered widespread clinical implementation. Since manual left-ventricular long-axis strain (LV LAS) has been successfully proposed as a simple alternative for LV deformation imaging, we aimed at the validation of left-atrial (LA) LAS.

Methods and results
The AIDA STEMI and TATORT-NSTEMI trials recruited 795 patients with ST-elevation myocardial infarction and 440 with non-ST-elevation myocardial infarction. LA LAS was assessed as the systolic distance change between the middle of a line connecting the origins of the mitral leaflets and either a perpendicular line towards the posterior atrial wall (LAS90) or a line connecting to the LA posterior portion of the greatest distance irrespective of a predefined angle (LAS). Primary endpoint was major adverse cardiac event (MACE) occurrence within 12 months. There were no significant differences between LA LAS and LAS90, both with excellent reproducibility. LA LAS correlated significantly with LA reservoir function (Es, r = 0.60, P &lt; 0.001). Impaired LA LAS resulted in higher MACE occurrence [hazard ratio (HR) 0.85, 95% confidence interval (CI) 0.82–0.88, P &lt; 0.001]. LA LAS (HR 0.90, 95% CI 0.83–0.97, P = 0.005) and LV global longitudinal strain (GLS, P = 0.025) were the only independent predictors for MACE in multivariate analyses. C-statistics demonstrated incremental value of LA LAS in addition to GLS (P = 0.016) and non-inferiority compared with FT Es (area under the receiver operating characteristic curve 0.74 vs. 0.69, P = 0.256).

Conclusion
Left-atrial LAS provides fast and software-independent approximations of quantitative LA function with similar value for risk prediction compared with dedicated deformation imaging.

Clinical trial registration
ClinicalTrials.gov: NCT00712101 and NCT01612312
Variation in fine-scale recombination rate in temperature-evolved Drosophila melanogaster populations in response to selection
Authors: Winbush Ari , Singh Nadia D 
Published: August 2022

Abstract
Meiotic recombination plays a critical evolutionary role in maintaining fitness in response to selective pressures due to changing environments. Variation in recombination rate has been observed amongst and between species and populations and within genomes across numerous taxa. Studies have demonstrated a link between changes in recombination rate and selection, but the extent to which fine-scale recombination rate varies between evolved populations during the evolutionary period in response to selection is under active research. Here, we utilize a set of 3 temperature-evolved Drosophila melanogaster populations that were shown to have diverged in several phenotypes, including recombination rate, based on the temperature regime in which they evolved. Using whole-genome sequencing data from these populations, we generated linkage disequilibrium-based fine-scale recombination maps for each population. With these maps, we compare recombination rates and patterns among the 3 populations and show that they have diverged at fine scales but are conserved at broader scales. We further demonstrate a correlation between recombination rates and genomic variation in the 3 populations. Lastly, we show variation in localized regions of enhanced recombination rates, termed warm spots, between the populations with these warm spots and associated genes overlapping areas previously shown to have diverged in the 3 populations due to selection. These data support the existence of recombination modifiers in these populations which are subject to selection during evolutionary change.
Predicting infrasound transmission loss using deep learning
Authors: Brissaud Quentin , Näsholm Sven Peter , Turquet Antoine , Le Pichon Alexis 
Published: August 2022

SUMMARY
Modelling the spatial distribution of infrasound attenuation (or transmission loss, TL) is key to understanding and interpreting microbarometer data and observations. Such predictions enable the reliable assessment of infrasound source characteristics such as ground pressure levels associated with earthquakes, man-made or volcanic explosion properties, and ocean-generated microbarom wavefields. However, the computational cost inherent in full-waveform modelling tools, such as parabolic equation (PE) codes, often prevents the exploration of a large parameter space, that is variations in wind models, source frequency and source location, when deriving reliable estimates of source or atmospheric properties—in particular for real-time and near-real-time applications. Therefore, many studies rely on analytical regression-based heuristic TL equations that neglect complex vertical wind variations and the range-dependent variation in the atmospheric properties. This introduces significant uncertainties in the predicted TL. In the current contribution, we propose a deep learning approach trained on a large set of simulated wavefields generated using PE simulations and realistic atmospheric winds to predict infrasound ground-level amplitudes up to 1000 km from a ground-based source. Realistic range dependent atmospheric winds are constructed by combining ERA5, NRLMSISE-00 and HWM-14 atmospheric models, and small-scale gravity-wave perturbations computed using the Gardner model. Given a set of wind profiles as input, our new modelling framework provides a fast (0.05 s runtime) and reliable (∼5 dB error on average, compared to PE simulations) estimate of the infrasound TL.
Molecular mechanism underlying impaired hepatic autophagy in glycogen storage disease type Ib
Authors: Gautam Sudeep , Zhang Lisa , Lee Cheol , Arnaoutova Irina , Chen Hung Dar , Resaz Roberta , Eva Alessandra , Mansfield Brian C , Chou Janice Y 
Published: August 2022

Abstract
Type Ib glycogen storage disease (GSD-Ib) is caused by a deficiency in the glucose-6-phosphate (G6P) transporter (G6PT) that translocates G6P from the cytoplasm into the endoplasmic reticulum lumen, where the intraluminal G6P is hydrolyzed to glucose by glucose-6-phosphatase-α (G6Pase-α). Clinically, GSD-Ib patients manifest a metabolic phenotype of impaired blood glucose homeostasis and a long-term risk of hepatocellular adenoma/carcinoma (HCA/HCC). Studies have shown that autophagy deficiency contributes to hepatocarcinogenesis. In this study, we show that G6PT deficiency leads to impaired hepatic autophagy evident from attenuated expression of many components of the autophagy network, decreased autophagosome formation and reduced autophagy flux. The G6PT-deficient liver displayed impaired sirtuin 1 (SIRT1) and AMP-activated protein kinase (AMPK) signaling, along with reduced expression of SIRT1, forkhead boxO3a (FoxO3a), liver kinase B-1 (LKB1) and the active p-AMPK. Importantly, we show that overexpression of either SIRT1 or LKB1 in G6PT-deficient liver restored autophagy and SIRT1/FoxO3a and LKB1/AMPK signaling. The hepatosteatosis in G6PT-deficient liver decreased SIRT1 expression. LKB1 overexpression reduced hepatic triglyceride levels, providing a potential link between LKB1/AMPK signaling upregulation and the increase in SIRT1 expression. In conclusion, downregulation of SIRT1/FoxO3a and LKB1/AMPK signaling underlies impaired hepatic autophagy which may contribute to HCA/HCC development in GSD-Ib. Understanding this mechanism may guide future therapies.
Polarity-driven laminar pattern formation by lateral-inhibition in 2D and 3D bilayer geometries
Authors: Moore Joshua W , Dale Trevor C , Woolley Thomas E 
Published: August 2022

Abstract
Fine-grain patterns produced by juxtacrine signalling have previously been studied using static monolayers as cellular domains. However, analytic results are usually restricted to a few cells due to the algebraic complexity of non-linear dynamical systems. Motivated by concentric patterning of Notch expression observed in the mammary gland, we combine concepts from graph and control theory to represent cellular connectivity and behaviour. The resulting theoretical framework allows us to exploit the symmetry of multicellular bilayer structures in 2D and 3D, thereby deriving analytical conditions that drive the dynamical system to form laminar patterns, consistent with the formation of cell polarity by activator localization. Critically, the patterning conditions are independent of the precise dynamical details, thus the framework allows for generality in understanding the influence of cellular geometry and signal polarity on patterning using lateral-inhibition systems. Applying the analytic conditions to mammary organoids suggests that intense cell signalling polarity is required for the maintenance of stratified cell types within a static bilayer using a lateral-inhibition mechanism. Furthermore, by employing 2D and 3D cell-based models, we highlight that the cellular polarity conditions derived from static domains can generate laminar patterning in dynamic environments. However, they are insufficient for the maintenance of patterning when subjected to substantial morphological perturbations. In agreement with the mathematical implications of strict signalling polarity induced on the cells, we propose an adhesion-dependent Notch-Delta biological process that has the potential to initiate bilayer stratification in a developing mammary organoid.