bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of arthroscopy-assisted localization technique in PFNA surgery
Authors: Sun Weijun 
Published: September 2022

Abstract
Objective: To investigate the efficacy of PFNA operation using arthroscopy.
Methods: Patients who visited our hospital for PFNA surgery from January 2011 to December 2017 were selected as the research subjects, and the patients were randomly divided into two groups. The control group performed conventional fluoroscopy-assisted positioning of needle entry points, and the observation group performed arthroscopic assisted positioning of needle entry points. The intraoperative fluoroscopy time, operation time, tip distance, fracture healing time, Harris score, incision length and length were recorded and compared in both groups.
Results: Both groups completed the operation successfully and had no perioperative death. Bone healing was achieved at at least 1 year, free of screw blade removal, and nonunion occurred in all the patients' follow-up. Two patients in the control group developed distal fractures, which healed after auxiliary fixation with the enveldevice. The operation time, intraoperative bleeding and fluoroscopy time in the observed group were less significant than in the control group. There were no significant differences in fracture healing time, TAD, and Harris score at the last follow-up between the two groups.
Conclusion: Arthroscopy-assisted needle insertion point positioning technology can reduce the operation time and bleeding time, and can simplify the procedure of PFNA surgery, which is worth further promotion.
Localized pseudo-subarachnoid hemorrhage
Authors: Sun Hui , Jian Sha , Dai Jing , Li Jingwei , Hou Jian , Peng Bo 
Published: September 2022

Abstract

Background: To analyze the imaging and clinical data of patients with localized pseudo-subarachnoid hemorrhage (pseudo-SAH), so as to improve the correct understanding of this sign by radiologists.
Methods: The imaging and clinical data of patients with localized pseudo-SAH on head CT scan without contrast from January 2013 to March 2022 were retrospectively analyzed, and the imaging and clinical characteristics were summarized.
Results: The etiology of 9 patients with pseudo-SAH was viral encephalitis (2 cases), acute cerebral infarction (4 cases) and hypoxic ischemic encephalopathy (3 cases). All patients underwent CT scan without contrast, CT scan with contrast, MRI and lumbar puncture examination. All patients' head CT scan without contrast showed local swelling of brain tissue (less than two lobes), stenosis and occlusion of adjacent subarachnoid space, with its inner strip of high-density shadow. CT scan with contrast found that the high density shadow was significantly enhanced. MRI and lumbar puncture were performed to rule out true SAH and confirm pseudo-SAH. Finally, 8 patients were discharged from hospital after treatment, and 1 patient died of multiple organ failure.
Conclusion: Localized pseudo-SAH often indicates localized brain lesions and has a relatively good prognosis. It has certain characteristics in CT value, enhancement mode and morphology.
Return of results from genomic research: a practical tool
Authors: Vears Danya , Hallowell Nina , Bentzen Heidi Beate , Ellul Bridget , Nøst Therese , Kerasidou Angeliki , Kerr Shona , Mayrhofer Michaela , Mežinska Signe , Ormondroyd Elizabeth , Solberg Berge , Sand Birgitte , Budin-Ljøsne Isabelle 
Published: September 2022

Abstract
An increasing number of European research projects return, or plan to return, individual genomic research results (IRR) to participants. While data access is a data subject’s right under the GDPR, and many legal and ethical guidelines allow or require participants to receive personal data generated in research, the practice of returning results is not straightforward and raises several practical and ethical issues. Existing guidelines focusing on return of IRR are mostly project-specific, only discuss which results to return, or were developed outside Europe. To address this gap, we analysed existing normative documents identified online using inductive content analysis. We used this analysis to develop a checklist of steps to assist European researchers considering whether to return IRR to participants. We then sought feedback on the checklist from an interdisciplinary panel of European experts (clinicians, clinical researchers, population-based researchers, biobank managers, ethicists, lawyers and policy makers) to refine the checklist. The checklist outlines seven major components researchers should consider when determining whether, and how, to return results to adult research participants: 1) Consider which results to return; 2) Develop a plan for return of results; 3) Obtain participant informed consent; 4) Collect and analyse data; 5) Confirm results; 6) Disclose research results; 7) Follow-up and monitor. Our checklist provides a clear outline of the steps European researchers can follow to develop ethical and sustainable result return pathways within their own research projects. Further legal analysis is required to ensure this checklist complies with relevant domestic laws.
What is the impact of nicotine on the Post-COVID-19 syndrome - a severe impairment of acetylcholine-orchestrated neuromodulation: A case series
Authors: Leitzke Marco 
Published: September 2022

Abstract
Following SARS-CoV-2 infection, many individuals suffer from post-COVID-19 syndrome. It makes them unable to proceed with ordinary everyday activities due to weakness, memory lapses, pain, dyspnea, and other unspecific physical complaints. Several investigators have demonstrated that the SARS-CoV-2-related spike glycoprotein (SGP) not only attaches to angiotensin-converting enzyme-2 (ACE-2) receptors but also shows DNA sections highly affine to nicotinic acetylcholine receptors (nAChRs). The nAChR is the principal structure of cholinergic neuromodulation and is responsible for coordinated neuronal network interactions. Nonintrinsic viral nAChR attachment substantially compromises integrative interneuronal communication. This explains the cognitive, neuromuscular, and mood impairment and the vegetative symptoms characterizing post-COVID-19 syndrome. The agonist ligand nicotine shows an up to 30-fold higher affinity for nACHRs than acetylcholine (ACh). We hypothesize that this molecule could extrude the virus from nAChR attachment and pave the way for unimpaired cholinergic signal transmission. Treating several individuals suffering from post-COVID-19 syndrome with a nicotine patch application, we observed an immediately substantial improvement of the symptoms up to complete remission after several days.
Service Quality &amp; its Impact on Citizen Service Satisfaction: With Reference to Governance in Karnataka, India
Authors: Padiyar Raghavendra D , R Sugant 
Published: September 2022

Abstract
Good Governance is essential for socio-economic sustainability. Delivery of government/citizen services to citizens is the main functionality of any government. For measuring the success of government and their governance well-structured theory is necessary which could assist governments to improve the quality of their services and identify the factors which could enhance the citizen service satisfaction. This research provides understanding about citizen perception of service quality with regard to Government of Karnataka’s service delivery system under ‘Right to Service Act’. The right to service act ensures in-time delivery of notified government/citizen services to citizens. The right to service is act in Karnataka is named as ‘The Karnataka Sakala Services Act, 2011 and (Amendment) Act, 2014’ popularly known as ‘Sakala’. The word Sakala is derived from state’s official language ‘Kannada’ which means in ‘in-time’ or ‘good time’. It has been over a decade since implementation of this act in the state and as on August 2022 the government of Karnataka has been delivering 1124 services through 99 government institutions under this act at intime disposition rate of 95.06%, highest ever in the country when compared with 24 other states and 5 union territories of India which have already adapted the Right to Service Act. However, the cumulative statistical evidence shows that the service rejection rate in Karnataka has been 6.41% and delayed disposition rate has been 4.94%. Therefore, it becomes necessary to gauge the quality of service delivered by Government of Karnataka under Right to service act. A survey was conducted and 1027 valid responses were received. The study found that factors Tangibility, Reliability, Responsiveness, Assurance and Empathy are the primary determinants of functional quality whereas the factors Valence & Waiting Time are the primary determinants of technical quality. Thus, functional quality and technical quality contributing as dimension of service quality. This research is of high value to scholars who are interested in the area of government service delivery system in India and other developing countries where government delivers its services to citizens through citizen charter, stipulated time line or through right to service act.
Acidic ascites inhibits ovarian cancer cell proliferation and correlates with the metabolomic, lipidomic and inflammatory phenotype of human patients
Authors: Yang Qianlu , Bae Gyuntae , Nadiradze Giorgi , Castagna Arianna , Berezhnoy Georgy , Zizmare Laimdota , Kulkarni Aditi , Singh Yogesh , Weinreich Frank J. , Kommoss Stefan , Reymond Marc A. , Trautwein Christoph 
Published: September 2022

Abstract
Background
The poor prognosis of ovarian cancer patients is strongly related to peritoneal metastasis with the production of malignant ascites. However, it remains largely unclear how ascites in the peritoneal cavity influences tumor metabolism and recurrence. This study is an explorative approach aimed at for a deeper molecular and physical-chemical characterization of malignant ascites and to investigate their effect on in vitro ovarian cancer cell proliferation.
Methods
This study included 10 malignant ascites specimens from patients undergoing ovarian cancer resection. Ascites samples were deeply phenotyped by 1H-NMR based metabolomics, gas flow analysis and a 13-plex cytokine panel. Characteristics of tumor cells were investigated in a 3D spheroid model by metabolic activity, adhesion, anti-apoptosis, migratory ability adhesion assay, flowcytometry and scratch assay. The effect of different pH values was assessed by adding 10% malignant ascites to the test samples.
Results
The overall extracellular (peritoneal) environment was alkaline, with pH of ascites at stage II-III = 7.51 ± 0.16, and stage IV = 7.78 ± 0.16. Ovarian cancer spheroids grew rapidly in a slightly alkaline environment. Decreasing pH of the cell culture medium suppressed tumor features, metabolic activity, adhesion, anti-apoptosis, and migratory ability. However, 10% ascites could prevent tumor cells from being affected by acidic pH. Metabolomics analysis identified stage IV patients had significantly higher concentrations of alanine, isoleucine, phenylalanine, and glutamine than stage II-III patients, while stage II-III patients had significantly higher concentrations of 3-hydroxybutyrate. Various positive and negative correlations were observed between physical-chemical parameters, polar/lipid metabolites, and cytokines.
Conclusion
Malignant ascites facilitated ovarian cancer progression. Additionally, deep ascites phenotyping by metabolomics and cytokine investigations allows for a refined stratification of ovarian cancer patients.
Improvement in erectile dysfunction in patients undergoing hyperbaric oxygen treatment: A prospective, double-blind, double controlled study
Authors: EKER Anıl , Celik Serdar , Ozer Elif , Basmaci Ismail , Sefik Ertugrul , Bozkurt Ibrahim , Gunlusoy Bulent , Degirmenci Tansu 
Published: September 2022

Abstract
Background: Erectile dysfunction (ED) is a worldwide health problem. Oral phosphodiesterase type 5 inhibitors (PDE5I) are used in its first-line treatment. This study aimed to compare the effects of hyperbaric oxygen (HBO) treatment with PDE5I treatment and determine the patient-dependent factors affecting the efficacy of the HBO treatment and duration of action of HBO treatment.
Methods: Adult male patients who presented to the HBO unit for HBO treatment with non-urological indications and had ED based on the International Erectile Function Index (IIEF-5) constituted the target population of this study. Participants were given HBO treatment (Group 1), no treatment (Group 2), or daily oral tadalafil 5 mg treatment (Group 3). The treatment duration was one month. Patients were assessed by IIEF-5 both initially and after the completion of one month.
Results: 102 male patients were included. Among these patients, 40 received HBO treatment, and 31 received daily oral 5 mg tadalafil treatment. Thirty-one patients were assigned to the control group (Group 2). There were significant increases in the mean IIEF-5 scores of the patients in Group 1 and Group 3 (p<0.001, p<0,001). However, there was no significant improvement in Group 2 (p=0,496). Also, the post-treatment IIEF-5 scores of Group 1 and Group 3 were significantly higher than Group 2 (p<0,001). There was no significant difference between the IIEF-5 scores and ∆IIEF-5 values of Group 1 and Group 3 (p=0,166, p=0,093). Evaluation regarding comorbidities revealed that patients with the peripheric vascular disease did not improve with HBO treatment (p=0,285). Analysis concerning the duration of action of HBO treatment revealed that treatment effects subsided in 90% of the patients six months after completion of treatment.
Conclusion: HBO can improve erectile functions, and it can be a reasonable alternative for patients who cannot use PDE5Is due to comorbidities or treatment side effects.
Clinical evaluation of malignancy diagnosis of rare thyroid carcinomas by an artificial intelligent automatic diagnosis system
Authors: Wang Yuan , Xu Lei , Lu Wenliang , kong Xiangkai , Shi Kaiyuan , Wang Liping , Kong Dexing 
Published: September 2022

Abstract
Purpose: To evaluate the application value of a generally trained artificial intelligence (AI) automatic diagnosis system in the malignancy diagnosis of rare thyroid carcinomas, such as follicular thyroid carcinoma, medullary thyroid carcinoma, primary thyroid lymphoma and anaplastic thyroid carcinoma and compare the diagnostic performance with radiologists of different experience levels.
Methods: We retrospectively studied 342 patients with 378 thyroid nodules that included 196 rare malignant nodules by using postoperative pathology as the gold standard, and compared the diagnostic performances of three radiologists (one junior, one mid-level, one senior) and that of AI automatic diagnosis system.
Results: The accuracy of the AI system in malignancy diagnosis was 0.825, which was significantly higher than that of all three radiologists and higher than the best radiologist in this study by a margin of 0.097 with P-value of 2.252×10 -16 . The mid-level radiologist and senior radiologist had higher sensitivity (0.857 and 0.959) than that of the AI system (0.847) at the cost of having much lower specificity (0.533, 0.478 versus 0.802). The junior radiologist showed relatively balanced sensitivity and specificity (0.816 and 0.549) but both were lower than that of the AI system.
Conclusions: The generally trained AI automatic diagnosis system showed high accuracy in the differential diagnosis of begin nodules and rare malignancy nodules. It may assist radiologists for screening of 3 rare malignancy nodules that even senior radiologists are not acquainted with.
A novel magnetic retrieval system for button battery in upper gastrointestinal
Authors: Wang Rongfeng , Lu Yiwen , Shan Liyu , Feng Zhe , Wang Yue , Zhao Meng , Wu Rongqian , Pang Lihui , Lv Yi 
Published: September 2022

Abstract
BACKGROUND: Endoscope can removes the button battery embedded in the esophagus，recommend emergency for symptomatic and asymptomatic children (< 2 hours) , For intragastric button batteries(BB), food debris may make positioning difficult. The smooth disc-shaped button battery allows foreign body forceps to grasp the battery Easy to slip off.Reasons for the delay include limited access to endoscopists, referral to tertiary care institutions, or delayed anesthesia due to gastric tamponade may all cause complications,In view of the extremely short effective treatment time window of the button battery in the upper gastrointestinal tract , this study designed a simple, fast and effective magnetic retrieval system for the button battery of the upper digestive tract.
Method: A set of magnetic retrieval system(MRS) for removing the upper digestive tract button battery was designed, including the magnetic retrieval device and the external guide magnet. We verified its feasibility by finite element analysis (FEA) simulation and removed button batteries with MRS in Beagle model.
Result: The button battery in the esophagus were all retrieved, No operation-related complications occurred. the retrieval time in the magnet group and endoscopic group was 92.91±82.47 s and 327.64±270.63 s respectively,P=0.012. 4 shifts happened in the endoscopic group during operation, 3 button batteries slipped off the forceps in the endoscopic group,resistance when retrieved was 2.1±1.02 N. The button battery in the stomach was all retrieved , the median time for magnetic aggregation using a guide magnet is 87s (70-135s), the median retrieved time was 21s (19-35s), and the resistance during the retrieved was 1.98N (1.67-2.4N).
Conclusion: we designed a novel magnetic retrieval system in this study. The MRS has a short magnetic retrieval time, is safe,cheapness and easy to operate, We suggest that the MRS could be used for the retrieval of BB during children ingestion button batteries.
The status of bone turnover markers in the male patients with osteoporosis
Authors: Zhuang Huafeng , Li Yimin , Li Yizhong , Yu Haiming , Wang Peiwen , Cai Siqing , Wang Benzhou 
Published: September 2022

Abstract
Objective To observe the change of bone turnover markers in the male patients with osteoporosis.
Methods We retrospectively reviewed the medical records including their original reports of bone mineral density , 25OHD, P1NP and β-CTXin the male patients with osteoporosis treated with bisphosphonates in our hospital between 2013and 2020. Serum 25OHD, P1NP and β-CTX were measured by radioimmunoassay. To study the impact of age on 25OHD, P1NP and β-CTX , the patients were divided into subgroups according to age: <60 yrs、60-69 yrs、70-79 yrs and ≥80 yrs. To study the impact of 25OHDon P1NP and β-CTX, the patients were divided into < 20 ng/ml、20-30 ng/ml and ≥ 30 ng/ml subgroups according to 25OHD level. Comparison of high bone turnover rate was made between the patients with fracture history and patients without fracture history.
Results There were 230 patients. 32 patients were aged <60 yrs, 59 patients were aged 60-69 yrs, 67 patients were aged70-79 yrs and 71 patients were aged ≥80 yrs. Serum 25OHD level was 3-70 ng/ml with average of 29.41±12.48 ng/ml. P1NP level was 11.5-100.8 ng/ml with average of 48.69±20.72 ng/ml. β-CTX level was 0.067-1.91 ng/ml with average of 0.522±0.282 ng/ml. P1NP and /or β-CTX above the upper normal range were present in 47.83% of the patients. The high bone turnover rate was significantly higher in the patients with fracture history than patients without fracture history. There was no impact of age and 25OHD level on the P1NP or β-CTX in our series.
Conclusion High bone turnover was common in male patients with primary osteoporosis.
Three-Dimensional Visualization of Dentine Occlusion Based on FIB-SEM Tomography
Authors: Du Ke , xu shiyou , Chen Xinye , Ryan Kaleigh , Hines Deon , Pan Long 
Published: September 2022

Abstract
The occlusion of dentinal tubules has become a rapid and effective method for treating dentin hypersensitivity. Accurate evaluation of dentin occlusion is critical to illustrate the efficacy of oral care products and to optimize dental therapy in the clinics, which is limited by the conventional two-dimensional (2-D) characterization methods. Here, we demonstrate the visualization of the dentin occlusion via three-dimensional (3-D) characterization using a focused ion beam-scanning electron microscopy (FIB-SEM) tomography. Using the “Slice and View” approach, the material used for occluding dentin tubules is imaged with a very high-resolution voxel (10 nm × 10 nm × 20 nm) from 2-D SEM images and then reconstructed into a 3-D volume, which presents the mode of action of toothpaste for treating dentin hypersensitivity. Meanwhile, quantitative analysis of the depth of occlusion is successfully obtained. This work validates the feasibility of FIB-SEM tomography in the analysis of dentin occlusion within the complicated networks of dentine tubules at the nanoscale, and provides a novel approach to facilitate the research and development of oral care products.
Prediction of Transition State Structures of General Chemical Reactions via Machine Learning
Authors: Choi Sunghwan 
Published: September 2022

Abstract
The elucidation of transition state (TS) structures is ssential for understanding the mechanisms of chemical reactions and exploring reaction networks. Despite advances in computational approaches, TS searches remain still a challenging problem due to the difficulty of constructing an initial structure and heavy computational costs. Herein, a novel machine learning (ML) model for predicting TS structures of general organic reactions is proposed. The proposed model derives interatomic distances of a TS structure from atomic pair features reflecting reactant, product, and linearly interpolated structures. The model shows excellent accuracy, particularly for the atomic pairs where bond formation or breakage occurs.
The predicted TS structures result in a high success ratio (93.8%) of quantum chemical saddle-point optimizations, and 88.8% of the optimization results have energy errors of less than 0.1 kcal/mol. Additionally, as a proof-of-concept, exploring multiple reaction paths of an organic reaction is demonstrated with the ML inferences. I envision that the proposed approach will aid the construction of initial geometry for TS optimization and reaction path explorations.
Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose
Authors: Ji Naizhe , Wang Qianyu , Li Shanshan , Wen Jiaxin , Wang Liangsheng , Ding Xiaohao , Zhao Shiwei , Feng Hui 
Published: September 2022

Abstract
Background
Petal blotch is a unique ornamental trait in angiosperm families, and blotch in petals of rose is rare and has great esthetic value.
Results
In this study, the rose cultivar 'Sunset Babylon Eyes' with rose-red to dark red blotch at the base of petals was selected as the experimental material. The HPLC-DAD and UPLC-TQ-MS analyses showed that only cyanidin 3,5-O-diglucoside (Cy3G5G) contributed to the blotch pigmentation of 'Sunset Babylon Eyes', and the amounts of Cy3G5G varied at different developmental stages. Only flavonols but no flavone were found in blotch and non-blotch parts, thereby kaempferol and its derivatives as well as quercetin and its derivatives may act as background color during flower developmental stages. Despite of the differences in composition, the total contents of carotenoids in blotch and non-blotch parts were similar, and carotenoids may just make the petals show a brighter color. Transcriptomic data and quantitative real-time PCR analyses indicated that RC7G0058400 (F3'H), RC6G0470600 (DFR), and RC7G0212200 (ANS) may be the key enzyme genes for the early formation and color deepening of blotch at later stages, and two transcription factor, RC7G0019000 (MYB) and RC1G0363600 (WRKY) may bind to the promoters of critical enzyme genes, or RC1G0363600 (WRKY) may bind to the promoter of RC7G0019000 (MYB) to activate the anthocyanin accumulation in blotch parts of 'Sunset Babylon Eyes'.
Conclusions
Our findings provide a theoretical basis for the understanding of the chemical and molecular mechanism for the formation of petal blotch in rose.