bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasmodium falciparum Drug Resistance Genes pfmdr1 and pfcrt In Vivo Co-Expression During Artemether-Lumefantrine Therapy
Authors: Silva M. , Malmberg M. , Otienoburu S. D. , Björkman A. , Ngasala B. , Mårtensson A. , Gil J. P. , Veiga M. I. 
Published: May 2022

Background: Artemisinin-based combination therapies (ACTs) are the global mainstay treatment of uncomplicated Plasmodium falciparum infections. PfMDR1 and PfCRT are two transmembrane transporters, associated with sensitivity to several antimalarials, found in the parasite food vacuole. Herein, we explore if their relatedness extends to overlapping patterns of gene transcriptional activity before and during ACT administration.Methods: In a clinical trial performed in Tanzania, we explored the pfmdr1 and pfcrt transcription levels from 48 patients with uncomplicated P. falciparum malaria infections who underwent treatment with artemether-lumefantrine (AL). Samples analyzed were collected before treatment initiation and during the first 24 h of treatment. The frequency of PfMDR1 N86Y and PfCRT K76T was determined through PCR-RFLP or direct amplicon sequencing. Gene expression was analyzed by real-time quantitative PCR.Results: A wide range of pre-treatment expression levels was observed for both genes, approximately 10-fold for pfcrt and 50-fold for pfmdr1. In addition, a significant positive correlation demonstrates pfmdr1 and pfcrt co-expression. After AL treatment initiation, pfmdr1 and pfcrt maintained the positive co-expression correlation, with mild downregulation throughout the 24 h post-treatment. Additionally, a trend was observed for PfMDR1 N86 alleles and higher expression before treatment initiation.Conclusion:pfmdr1 and pfcrt showed significant co-expression patterns in vivo, which were generally maintained during ACT treatment. This observation points to relevant related roles in the normal parasite physiology, which seem essential to be maintained when the parasite is exposed to drug stress. In addition, keeping the simultaneous expression of both transporters might be advantageous for responding to the drug action.
Study on the Insulation Performance of SF6 Gas Under Different Environmental Factors
Authors: An Yunzhu , Yin Kaiqiang , Huang Tao , Hu Yuanchao , Ma Chenghui , Yang Minghao , An Bingchen , Chen Dan 
Published: May 2022

During the long-term operation of electrical equipment, the insulation performance of applied SF6 gas may be affected by humidity, pressure, and temperature. In this study, the collision cross sections of SF6 and H2O are considered, including ionization, attachment, excitation, and elastic collision. In order to analyze the influencing factors of SF6 insulation performance, discharge parameters are computed by the Boltzmann equation under different environmental conditions, including humidity, pressure, and temperature. It is concluded that with the conditions of 0.1 MPa and 300 K, the critical breakdown electric field increases by 1.23 kV/cm as the relative humidity increases from 0% to 80%. As the pressure increases from 0.1 to 0.6 MPa, the critical breakdown electric field increases by 451.07 kV/cm. However, temperature also has little effect on the insulation performance of SF6 gas. The conclusions of this study have reference value for safe and stable operation of SF6 insulated power equipment.
Anthropometrical and Physiological Determinants of Laboratory and on-Snow Performance in Competitive Adolescent Cross-Country Skiers
Authors: Sollie Ove , Losnegard Thomas 
Published: May 2022

Purpose: To explore the anthropometrical and physiological determinants of laboratory and on-snow performance in competitive adolescent cross-country skiers.Methods: Fifty-two adolescent (25 girls) (14.8 ± 0.6 years) skiers performed an uphill treadmill rollerski session using the G2 ski skating technique. Gross efficiency (GE) was calculated from a submaximal work bout (∼84% of peak oxygen uptake; V̇O2peak) while V̇O2peak, accumulated oxygen deficit (ΣO2def) and laboratory performance were determined from a 3-min time trial (TT3min) before upper- and lower-body maximum strength were tested. Pearson’s product moment correlations and multiple regression analysis explored the relationship with anthropometrical and physiological determinations of laboratory and on-snow performance in sprint (∼1 km, ∼2.5–3 min) and distance races (5–7.5 km, ∼12–20 min) from the national championship for this age-group.Results: A large correlation was found between on-snow sprint and distance performance (boys r = 0.61, girls r = 0.76, both p &lt; 0.01) and for on-snow distance performance with TT 3min (r = 0.51 to 0.56, p &lt; 0.05). V̇O2peak, ΣO2def and GE explained ∼80% of variations in performance in the TT3min, but substantial lower on-snow skiing performance (∼20–30%). For the TT3min performance, V̇O2peak showed a very large and large correlation for boys and girls (r = 0.76 and 0.65 respectively, both p &lt; 0.01), ΣO2def showed a large correlation for boys and girls (r = 0.53 and 0.55 respectively, both p &lt; 0.01) and age showed a large correlation for boys (r = 0.56, p &lt; 0.01), with no significant correlation for girls (r = -0.19). For on-snow distance performance, V̇O2peak showed a large correlation for boys (r = 0.53, p &lt; 0.01) and girls (r = 0.50, p &lt; 0.05). For on-snow sprint performance, upper-body strength (r = 0.55, both sexes p &lt; 0.01) and body mass index (BMI) showed a large correlation for boys (r = 0.53, p &lt; 0.01) and girls (r = 0.51, p &lt; 0.05).Conclusion: V̇O2peak is an important determinant for overall XC skiing performance in competitive male and female adolescent skiers. However, upper-body strength and BMI correlate the most with sprint performance. While laboratory performance can to a large extent be explained by physiological factors, on-snow-performance for adolescents is based more on multivariate factors (tactics, equipment’s, technique, racecourse etc.), implying the need for a holistic approach to understanding the sport-specific demands in such age-groups.
Regular Physical Activities Inhibit Risk Factors of the Common Cold Among Chinese Adults
Authors: Tu Renjie , Lu Yifan , Tao Kuan 
Published: May 2022

BackgroundPhysical activity (PA) has a significant health impact worldwide and has been linked to a lower risk of the common cold.ObjectiveThe aim of this study was to estimate the form of PA among Chinese adults and the correlation between PA and number of the common cold in China's eastern, central, and western areas.DesignA cross-sectional study.SettingChina's eastern, central, and western regions from 30 November 2020 to 30 March 2021.PatientsA total of 1,920 healthy participants, who aged over 18 years old, with Internet access, were enrolled, and then self-reported PA behaviors and number of the common cold were collected.MeasurementsThe authors calculated preference, intensity, frequency, and duration of PA in Chinese based on gender, age, and broad occupational categories and explored the potential effect between these factors and the common cold.ResultsApproximately 20.4% of participants reported not participating in sports regularly. Except for gender, there were significant differences in PA preference and intensity among the remaining individuals (P &lt;0.05). Sixteen common exercises were divided into three intensity levels by the Borg CR10 Scale: low- (5), moderate- (8), and high-intensity exercises (3), and the corresponding intensity, frequency, and duration were computed with significant differences (P &lt;0.05). The most popular workouts are “Brisk walking” and “Running.” Age, sex, and occupation had no significant effect on colds (P &gt; 0.05). However, intensity shows a U-shaped dose-response relationship with colds, whereas the frequency and duration have an inverse dose-response relationship (P &lt;0.05). High intensity combined with high frequency increased colds the most. Nevertheless, non-exercise groups always have the most colds in each comparison.LimitationsThe result may be vulnerable to recall bias.ConclusionIntensity showed that U-shape, frequency, and duration showed inverse response to the number of colds last year, but age, sex, and occupation had no significant effects. High intensity and high frequency mixed increased colds the most, regardless of duration.
Accelerated Intermittent Theta Burst Stimulation in Smoking Cessation: Placebo Effects Equal to Active Stimulation When Using Advanced Placebo Coil Technology
Authors: Mikellides Georgios , Michael Panayiota , Psalta Lilia , Stefani Artemis , Schuhmann Teresa , Sack Alexander T. 
Published: May 2022

Smoking is currently one of the main public health problems. Smoking cessation is known to be difficult for most smokers because of nicotine dependence. Repetitive transcranial magnetic stimulation (rTMS) over the left dorsolateral prefrontal cortex (DLPFC) has been shown to be effective in the reduction of nicotine craving and cigarette consumption. Here, we evaluated the efficacy of accelerated intermittent theta burst stimulation (aiTBS; four sessions per day for 5 consecutive days) over the left DLPFC in smoking cessation, and we investigated whether the exposure to smoking-related cues compared to neutral cues during transcranial magnetic stimulation (TMS) impacts treatment outcome. A double-blind, randomized, controlled study was conducted in which 89 participants (60 males and 29 females; age 45.62 ± 13.42 years) were randomly divided into three groups: the first group received active aiTBS stimulation while watching neutral videos, the second group received active aiTBS stimulation while watching smoking-related videos and the last group received sham stimulation while watching smoking-related videos. Our results suggest that aiTBS is a tolerable treatment. All treatment groups equally reduced cigarette consumption, nicotine dependence, craving and perceived stress. The effect on nicotine dependence, general craving and perceived stress lasted for at least 1 week after the end of treatment. Active aiTBS over the left DLPFC, combined with smoking related cues, is as effective as active aiTBS combined with neutral cues as well as placebo aiTBS in smoking cessation. These findings extend the results of previous studies indicating that TMS therapy is associated with considerably large placebo effects and that these placebo effects may be further increased when using advanced placebo coil technology.Clinical Trial Registrationwww.clinicaltrials.gov, identifier NCT05271175.
Bovine In Vitro Oocyte Maturation and Embryo Production Used as a Model for Testing Endocrine Disrupting Chemicals Eliciting Female Reproductive Toxicity With Diethylstilbestrol as a Showcase Compound
Authors: Asimaki K. , Vazakidou P. , van Tol H. T. A. , Oei C. H. Y. , Modder E. A. , van Duursen M. B. M. , Gadella B. M. 
Published: May 2022

Endocrine disrupting chemicals (EDCs) can interfere with normal hormonal action and regulation. Exposure of women to EDCs has been associated with adverse reproductive health outcomes. The assays currently used to identify EDCs that elicit female reproductive toxicity lack screening tests that address effects on the maturation of oocytes, a process that enables them to be fertilized and develop into embryos. Here, a screening method employing the bovine model of in vitro oocyte maturation and embryo production is described. Endpoints explored address important events in oocyte maturation and developmental competence acquisition. To test the method, the effects of the known human EDC diethylstilbestrol (DES; an estrogen receptor agonist) were evaluated in a range of concentrations (10–9 M, 10–7 M, 10–5 M). Bovine oocytes were exposed to DES during in vitro maturation (IVM) or embryos were exposed during in vitro embryo culture (IVC). The endpoints evaluated included nuclear maturation, mitochondrial redistribution, cumulus cell expansion, apoptosis, and steroidogenesis. DES-exposed oocytes were fertilized to record embryo cleavage and blastocyst rates to uncover effects on developmental competence. Similarly, the development of embryos exposed to DES during IVC was monitored to assess the impact on early embryo development. Exposure to 10–9 M or 10–7 M DES did not affect the endpoints addressing oocyte maturation or embryo development. However, there were considerable detrimental effects observed in oocytes exposed to 10–5 M DES. Specifically, compared to vehicle-treated oocytes, there was a statistically significant reduction in nuclear maturation (3% vs 84%), cumulus expansion (2.8-fold vs 3.6-fold) and blastocyst rate (3% vs 32%). Additionally, progesterone and pregnenolone concentrations measured in IVM culture media were increased. The screening method described here shows that bovine oocytes were sensitive to the action of this particular chemical (i.e., DES), albeit at high concentrations. In principle, this method provides a valuable tool to assess the oocyte maturation process and early embryo development that can be used for reproductive toxicity screening and possibly EDC identification. Further studies should include EDCs with different mechanisms of action and additional endpoints to further demonstrate the applicability of the bovine oocyte model for chemical risk assessment purposes and EDC identification.
Identification of Modulators of the C. elegans Aryl Hydrocarbon Receptor and Characterization of Transcriptomic and Metabolic AhR-1 Profiles
Authors: Larigot Lucie , Bui Linh-Chi , de Bouvier Marine , Pierre Ophélie , Pinon Grégory , Fiocca Justine , Ozeir Mohammad , Tourette Cendrine , Ottolenghi Chris , Imbeaud Sandrine , Pontoizeau Clément , Blaise Benjamin J. , Chevallier Aline , Tomkiewicz Céline , Legrand Béatrice , Elena-Herrmann Bénédicte , Néri Christian , Brinkmann Vanessa , Nioche Pierre , Barouki Robert , Ventura Natascia , Dairou Julien , Coumoul Xavier 
Published: May 2022

The Aryl hydrocarbon Receptor (AhR) is a xenobiotic sensor in vertebrates, regulating the metabolism of its own ligands. However, no ligand has been identified to date for any AhR in invertebrates. In C. elegans, the AhR ortholog, AHR-1, displays physiological functions. Therefore, we compared the transcriptomic and metabolic profiles of worms expressing AHR-1 or not and investigated the putative panel of chemical AHR-1 modulators. The metabolomic profiling indicated a role for AHR-1 in amino acids, carbohydrates, and fatty acids metabolism. The transcriptional profiling in neurons expressing AHR-1, identified 95 down-regulated genes and 76 up-regulated genes associated with neuronal and metabolic functions in the nervous system. A gene reporter system allowed us to identify several AHR-1 modulators including bacterial, dietary, or environmental compounds. These results shed new light on the biological functions of AHR-1 in C. elegans and perspectives on the evolution of the AhR functions across species.
Liderazgo gerencial para el desempeño laboral de los trabajadores
Authors: Malca Florindes Jorge Antonio , Callao Alarcón Marcelino 
Published: May 2022

El Liderazgo gerencial se ha convertido en un pilar fundamental para las organizaciones, ya que, a través de ello, se puede alcanzar un eficiente desempeño laboral de cada uno de los integrantes de la entidad. Razón por la cual, este estudio se planteó como objetivo proponer un modelo de liderazgo gerencial que contribuya al fortalecimiento del desempeño laboral de los trabajadores de la Municipalidad Provincial de Jaén, 2021. Se utilizó un enfoque cuantitativo, no experimental transaccional, de tipo básica – propositivo, la técnica fue la encuesta y el instrumento el cuestionario, el cual, fue aplicado a 91 trabajadores de la Municipalidad Provincial de Jaén. Los resultados alcanzados revelaron que el 63% manifestaron que los funcionarios casi nunca reiteran a los empleados a cumplir cabalmente con el reglamento interno, asimismo, se observó que el 73% revelan que nunca los funcionarios y jefes de área toman las medidas correctivas cuando el personal demuestra ineficiencia en sus funciones. La propuesta contiene fundamentos filosóficos y conceptuales que permitirán mejorar el desempeño laboral de los empleados de esta entidad, porque se requieren de personas con un enfoque diferente de la realidad, una confianza y determinación que puedan tener una autoconfianza subordinada y unir con entusiasmo los esfuerzos del equipo y los objetivos organizacionales compartidos. En tiempos diferentes, los gerentes con liderazgo gerencial transformador que pueda sensibilizar a los trabajadores de lo que deben hacer y valorar.
Clima organizacional en las instituciones gubernamentales
Authors: Ramírez Cueva Rensso Janpier , Cobeñas Fernández Claudia Mirella 
Published: May 2022

El clima organizacional es el conjunto de propiedades que son medibles acerca del espacio de trabajo que se percibido, en forma directa o indirectamente, de aquellas personas que trabajan en un ambiente y éstos influyen en la motivación y el comportamiento en ese espacio laboral. Aspectos significativos para el propósito de este estudio que fue diagnosticar el clima organizacional en las instituciones gubernamentales, la metodología de naturaleza cuantitativa abordó el tipo de investigación diagnóstica y el diseño de campo; las unidades muestrales estuvieron representadas por 84 trabajadores municipales. Se utilizó la técnica de la encuesta y se administró un cuestionario válido y confiable para la recolección de datos. Los resultados expresan que los espacios físicos, la estructura organizativa, el ambiente socioafectivo y los comportamientos organizacionales presentan un nivel Deficiente (19%), Regular (82,1%) y Bueno (32,1%) deduciendo que estos componentes vienen alterando la buena convivencia. Las conclusiones significativas describen que el clima organizacional en las instituciones gubernamentales del municipio de Chiclayo es adverso debido que los ambientes y áreas de trabajo se encuentran masificadas y no ofrecen las condiciones básicas para realizar la actividades administrativas, técnicas y gerenciales; asimismo, las interacciones interpersonales, decisiones y comportamientos profesionales carecen de consenso, empatía y regulación emocional.
The role of geriatric services in the diagnosis and monitoring of outcomes of postcoital syndrome ()
Authors: Лаптева Е.С. , Арьев А. Л. , Арьева Г.Т. , Цуцунава М.Р. , Дьячкова-Герцева Д.С. 
Published: May 2022

Помимо быстрого и волнообразного распространения пандемии по всему земному шару, сегодня все более масштабной становится проблема, называемая «долгий COVID-19», - этим термином описываются долгосрочные последствия новой коронавирусной инфекции. Как протекает болезнь после «выздоровления», включая отдаленные физические и психологические последствия для здоровья, и будущее выживших после COVID-19 остается в значительной степени неясным. Особенно это касается контингента старших возрастных групп. В данной статье обобщается опыт зарубежных исследований, нацеленных на определение длительности и уточнения характера полиорганных осложнений после перенесенного COVID -19, а также пути долгосрочной реабилитации пациентов старших возрастных групп с постковидным синдромом.
In addition to the rapid and rippling spread of the pandemic across the globe today, there is a growing problem called «long-COVID-19», a term that describes the long-term effects of a new coronavirus infection COVID-19. How the disease proceeds after «recovery», including the long-term physical and psychological health consequences, the future of COVID-19 survivors remains largely unclear. This is especially true for the contingent older age groups. This article summarizes the experience of foreign studies aimed at determining the duration and clarifying the nature of multi-organ complications after undergoing COVID-19, as well as ways of long-term rehabilitation of patients in older age groups with post-COVID syndrome.
Experiencias comunitarias de conservación y defensa del patrimonio cultural: El District Six Museum, Ciudad del Cabo, Sudáfrica
Authors: Efron Laura , Pineau Marisa 
Published: May 2022

Entre 1948 y 1994, los gobiernos del apartheid en Sudáfrica desplazaron de forma forzada a miles de personas no blancas que vivían en ciertas zonas urbanas e impusieron la residencia exclusiva de la minoría blanca. En 1966, más de 60.000 personas que vivían en el Distrito Seis de Ciudad de Cabo sufrieron los desplazamientos forzados impuestos por el Estado.
La Fundación del Museo del Distrito Seis surgió en 1989 como un proyecto comunitario que trabaja en pos de la revalorización de las historias silenciadas por el apartheid. El proyecto se propuso disputar los derechos a la propiedad de la tierra y buscar medidas de compensación por la desposesión y el trauma vivido durante el apartheid.
En este trabajo nos proponemos describir las distintas facetas del proyecto del museo que promueven no solo la protección del Patrimonio Cultural del Distrito Seis sino también la restitución de tierras a las familias que fueron forzosamente desplazadas. Así, buscamos revalorizar una experiencia de producción colectiva de la memoria cuyo impacto en la actualidad local es sustancial en pos del desarrollo de políticas públicas reparativas.
Reseñas
Authors: Piro Mittelman Gabriel , Murphy Jessica Priscila , Martín Lucio Emmanuel 
Published: May 2022

González Canosa, M. (2021). Los futuros del pasado. Marxismo, peronismo y revolución: una historia de las FAR. Prometeo Libros: Ciudad Autónoma de Buenos Aires, 298 páginas.

Camarero, H. y Ceruso, D. (2020). Comunismo y clase obrera hasta los orígenes del peronismo. Eudem- Grupo Editor Universitario: Ciudad Autónoma de Buenos Aires, 128 páginas.

Tortti, M. C. y González Canosa, M. (2021). La nueva izquierda en la historia reciente argentina. Prohistoria Ediciones: Rosario, 316 páginas.
مقترح المٌعلم الكتروني للمهام التعليمية لتفعيل التعلم المعاصر من وجهة نظر معلمي ومعلمات المدارس الثانوية
Authors: أ.د / حيدر حسن اليعقوبي  
Published: May 2022

صار الاهتمام اليوم بالتعلم الالكتروني ضرورة قصوى غير ان مسؤوليات المعلمة والمعلمة غير التعليمية يمكن ان تؤثر على سير عملية التعلم الالكتروني. فظاهرة الاهتمام بالتعليم الالكتروني البديل عن المعلم من خلال وجود معلم الكتروني يمكن ان يكون نافعا في الوقت الحالي لسد الكثير من الاحتياجات اللازمة لذلك ومن هذا يمكن تمثيل مشكلة البحث الحالي في السؤال الاتي : ماهي توجهات معلمي ومعلمات المدرسة الثانوية نجو مقترح المعلم الالكتروني. وهدف البحث الى : مقترح المٌعلم الكتروني للمهام التعليمية لتفعيل التعلم المعاصر من وجهة نظر معلمي ومعلمات المدارس الثانوية . والفرق ذا دلالة مدرسي ومدرسات الممارسين للتعليم الكتروني في مدينة كربلاء (مركز المدينة وأطرافها) في مديرية تربية كربلاء. للعام المدرسي (2022-2021).إستخدم الباحث منهج البحث الوصفي. إشتمل مجتمع البحث الحالي من معلمي ومعلمات بحسب الدرجة, درجة بكالوريوس (523) ودرجة عليا(95) , وبحسب التوزيع مركز المدينة (350) وأطراف المدينة (268) وللعام الدراسي (2021-2022) وبهذا تألف المٌجتمع الأصلي من (618) معلم ومتعلمة موزعين على مركز المدينة وأطرافها. بعد التحقق من الخصائص القياسية المتمثلة بمؤشرات الصدق والثبات والتحليل الإحصائي للمقياس أصبح لإستبانة المٌعلم الكتروني للمهام التعليمية للواقع بصيغته النهائية مكوناً من (14) فقرة موزعة على ثلاث مكونات هي: المعرفية التنظيمية الفاعلية التواصلية والقيمة التقديرية, وفق مدرج ثلاثي للاستجابة هو: [مهمة، مهمة الى حد ما، غير مهمة]. وتعطى عند تصحيح الدرجات وتعطى عند تصحيح الدرجات من درجة (1) ادنى درجة، ودرجة (3) أعلى درجة. أعلى قيمة (42) وأقل قيمة (14) بوسط فرضي لكل منهما (28). توصل البحث الى

يوجد توجه نحو إستعمال الية المعلم الالكتروني
يوجد اختلاف بين المعلمين في التوجه نحو مقترح المعلم الالكتروني
يوجد اختلاف بين الدرجة العلمية في التوجه نحو مقترح المعلم الالكتروني
لايوجد اختلاف بين التوزيع الجغرافي في التوجه نحو مقترح المعلم الالكتروني