bluuebunny/crossref_metadata_2025_split huggingface dataset:
The systematical actions of Resveratrol against Nasopharyngeal Carcinoma Based on Network Target Prediction and Experimental Verification
Authors: Zhou Bo , Xu Zihan , Man Tengjun , Yi Yujuan , Tang Hong , Li Jia , Sun Zheng 
Published: March 2022

Abstract
In this study, we aimed to find the targets of resveratrol in nasopharyngeal carcinoma (NPC) by network target prediction and investigate the possible mechanisms through experimental verification. Firstly, resveratrol and NPC-specific targets were combined with protein-protein interactions to construct a systematical resveratrol targets-NPC network. Consistently, molecular docking was performed to predict the molecular determinants of resveratrol against NPC. In order to clarify this point, the anti-cancer effects of resveratrol were investigated in the CNE2 cell lines in vitro and NPC cases in vivo. Functional enrichment results suggested that resveratrol played anti-NPC role mainly through MAPK pathway and the potential targets were MAPK1, MAPK3, STAT3, TP53, PIK3CA, PIK3R1, SRC, MAPK8, RELA and VEGFA. In vitro, resveratrol inhibited proliferation, invasion, migration and eventually induced apoptosis in CNE2 cells on time-and dose-dependent manner. Meanwhile, western blot confirmed that resveratrol exerted apoptotic activity of NPC cells by inhibiting MAPK cell signaling activation. Clinically, Immuno-histochemical staining showed significant differential p-ERK1/2, p-JNK and p-P38 activation between tumor-surrounding and NPC cases. Altogether, our findings identified resveratrol could play potent inhibition and apoptosis on human NPC cells possibly by targeting MAPK pathways and suggested that resveratrol had the potential to be an effective bioactive compound in NPC patients.
Association analysis of IKZF4 gene with alopecia areata in Chinese Han population
Authors: Miao Ying , Hu Ruiming , Sheng Youyu , Qi Sisi , Yang Qinping 
Published: March 2022

Abstract
Background: The IKZF4 gene encodes Eos, a zinc finger transcription factor that belongs to the Ikarosfamily. High expression of Eos on Treg cells is important for the suppression of autoimmune responses and immune homeostasis. It has been suggested that the SNP in IKZF4 may influence the pathogenesis of AA.Aims and Objectives: The purpose of this study was to explore the relationship between IKZF4 polymorphism and AA in Chinese Han population.Materials and Methods: In this study, we examined 459 patients and 434 controls using HRM analysis and direct sequencing to assess.the rs1701704 polymorphism.. Results: The prevalence of the C/C, A/C and A/A genotypes was 7.4%, 37.5% and 55.1%, respectively. There were significant differences in genotype distribution and allele frequencies between AA and control group (p<0.0001). The frequency of C allele in AA group was significantly higher(p<0.0001), and the frequencies of C allele and C/C genotype in patients with family history were higher (p<0.0001;p=0.001;).Conclusions: The rs1701704 SNP of IKZF4 may be a genetic marker for assessing the risk of AA in Chinese Han population.
Therapeutic Effects of Jiedu Huoxue Decoction in Chronic Prostatitis/Chronic Pelvic Pain Syndrome: Network Pharmacology, Molecular Docking and Experimental Evidence
Authors: Pan Junqing , Hu Xiangjun , Zhang Sina , Yin Hongwei , Zhang Congzhong , Deng Siqiao , Liu Can , Yan Zhangren 
Published: March 2022

Abstract
Background: Jiedu Huoxue decoction (JDHXD) can be effectively alleviated chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) patients’ pelvic pain, lower urinary tract symptoms, and other discomforts. But its mechanism of action involving multi-component, multi-target and multi-pathway is unclear. Objectives: Integrated strategy of network pharmacological prediction, molecular docking, and experimental validation elucidate potential mechanism of JDHXD in treating CP/CPPS.Study design and methods: Two traditional Chinese medicine systems pharmacology databases and three disease related databases were applied to identify the herbs active components and the putative targets of CP/CPPS. Using the Cytoscape to constructed and analysis the herbs-compounds-targets network. Subsequently, the protein-protein interaction (PPI) was investigated by STRING database. Using DAVID databases to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. The binding of core active compounds with hub targets were assessed by molecular docking. Furthermore, in vivo efficacy of JDHXD was validated in CP/CPPS rat model. The key signal pathway of network pharmacological prediction would be validated by animal experiment.Result: In total, 149 active compounds of 10 Chinese herbals and 909 potential CP/CPPS-related targets were identified. Network pharmacology revealed 161 common targets between 136 potential active compounds and CP/CPPS. After topology analysis, 5 core active compounds and 18 hub targets have been screened. PPI analysis obtained 18 hub targets. Based on enrichment analysis of 161 targets, we obtained GO function 277 items and KEGG pathway 114 items. Molecular docking verification shown that the top 5 hub targets were well binding with 5 core active compounds. Animal experiment shown that JDHXD had protective effect on CP/CPPS. JDHXD can significantly down-regulated the key gene expression (JNK, ERK1/2 and P38) and inhibit phosphorylation of ERK1/2，JNK and P38 in the MAPK signaling pathway.Conclusions: This study demonstrated potential mechanism of JDHXD in treating CP/CPPS. Network pharmacological, molecular docking and experimental verification revealed its multi-component, multi-target and multi-pathway treatment characteristics. The reliability and effectiveness of those method are very helpful to explore the material basis and mechanism of traditional Chinese medicine.
Social learning disfavours the cultural evolution of altruistic punishment
Authors: Burton-Chellew Max , D'Amico Victoire 
Published: March 2022

Abstract
Could human cooperation be the result of evolution favouring groups where altruists punish non-altruists 1-4? While punishment can benefit groups through increased cooperation, it often costs more than it is worth, challenging group level explanations 1,5,6. We therefore tested if altruistic punishment could spread among groups via imitation (social learning) rather than direct competition. Groups observed either how punishment increased cooperation, or how it also decreased profits, in a previous experiment. Individuals then voted to either enable or disable all punishment. We found (1) that punishment was common, but most individuals voted to disable punishment and (2) observing how punishment affected payoffs drastically disfavoured punishment, and consequently cooperation. Our results contradict the hypothesis that humans have evolved to cooperate via cultural group selection favouring altruistic punishment 2,3,7-11.
An improved monkey model of myocardial ischemic infarction for cardiovascular drug development
Authors: Wang Keke , Han Pengfei , Huang Lu , Xiao Ying , Hou Jianglong , Yang Pingliang , Xie Yuping , Cai Jindan , Wang Hongge , Kang Y. James 
Published: March 2022

Abstract
Non-human primate monkey model of myocardial ischemic infarction is precious for translational medicine research. Ligation of the left anterior descending (LAD) artery is the effective way to induce myocardial ischemic infarction. However, the consistency of the myocardial infarction thus generated remains problematic. The present study was undertaken to critically evaluate the monkey model of myocardial ischemic infarction to develop a procedure for a consistent cross-studies comparison. Forty male Rhesus monkeys were divided into 4 groups and subjected to LAD artery ligation at different levels along the artery. In addition, the major diagonal branch was selectively ligated parallel to the ligation site of the LAD artery according to the diagonal branch distribution. Analyses of MRI, echocardiography, cardiac hemodynamics, electrocardiography, histopathology, and cardiac injury biomarkers were undertaken to characterize the structural and functional alterations of monkeys with myocardial infarction. Ligation at 40% of the total length of the artery, measured from the apex end, produced variable infarct areas with inconsistent functional alterations. Ligation at 60% or above coupled with selective ligation of diagonal branches produced consistent myocardial infarction with uniformed dysfunction. However, ligation at 70% caused a lethal threat. After a thorough analysis, it is concluded that ligation at 60% of the total length coupled with selective ligation of diagonal branches, which enables standardization of the location of occlusion and the subsequent ischemic area, as well as avoids the influence of the diagonal branches, are ideal to produce a consistent monkey model of myocardial ischemic infarction.
A new scheme for calculating soil thermal diffusivity
Authors: Li Yujie , Gao Xiaoqing , Yang Liwei , Li Zhenchao , Zhou Ya 
Published: March 2022

Abstract
Land-atmosphere interaction is one of the important causes of climate change. The accurate characterization of physical mechanism of the soil layer can help us better understand the land-atmosphere interaction. As one of the important thermal properties of soil, soil thermal diffusivity is an important parameter reflecting soil thermal conductivity and a necessary condition for simulating soil thermodynamic environment. Based on the comparison of several methods for calculating soil thermal diffusivity (amplitude method, phase method, logarithmic method, arctangent method, Laplace method and numerical methods), this paper develops a new soil thermal diffusivity model (Thermal conduction-convection method based on Fourier series boundary condition for calculating soil thermal diffusivity, TCCMF) and compares the results of the new model with other methods. The results show that: (1) The accuracy of the results obtained by the amplitude method, phase method, logarithmic method, and arctangent method is not high enough; the Laplace method can better solve the effects of extreme weather or non-periodic changes in soil temperature. (2) When the numerical methods are used to solve the thermal conduction equation, the Crank-Nichalson-Sch format is unconditionally stable, the data utilization rate is higher, the obtained soil thermal diffusivity is less discrete, and the result is more accurate. (3) The new model (TCCMF) uses the Fourier series method, which is easy to calculate, and has more complete physical process and more flexible precision control. The simulation results of soil temperature using this method show that this method (TCCMF) has better simulation accuracy, indicating that it has potential value in simulating soil thermodynamic characteristics and land-atmosphere interaction.
Influence of Dorper lamb development from birth to 120 days of age on clinical and echocardiographic parameters
Authors: Aleixo Amanda Sarita Cruz , Ferreira Danilo Otávio Laurenti , Tsunemi Miriam Harumi , Chiacchio Simone Biagio , Lourenço Maria Lucia Gomes 
Published: March 2022

Abstract
The expansion of the sheep model in research represents an attractive and economically beneficial academic reason for investigations in sheep echocardiography. The present study aimed to evaluate the clinical and echocardiographic parameters in Dorper lambs during the developmental period. Emphasis was placed on the use of the species in translational research for the echocardiographic diagnosis of congenital heart diseases, which can contribute to improvements in interventionist techniques. Ten Dorper lambs were evaluated in the following periods: 24 hours after birth, 7, 14, 21, 30, 60, 90 and 120 days of age. Clinical parameters were compiled and echocardiogram records were performed without sedation. From 21 days of age, the heart rate (HR) decreased with a difference between moments. The rectal temperature differed from 7 days, there was a gradual increase. Mean and systolic blood pressure differed, with the highest values at 90 and 120 days of age. The thickness of interventricular septum in diastole (SIVd) increased as age progressed, with the highest value at 120 days of age, and the same occurred for DIVEd (left ventricle diameter in diastole), PLVEd (left ventricular free wall thickness in diastole), SIVs (interventricular septum thickness in systole), DIVEs (left ventricle diameter in systole) and PLVEs (left ventricular free wall thickness in systole). There were differences in the size of the AE, Ao and AE/Ao ratio, which were greater at 90 days and 120 days of age. Echocardiographic changes accompany the development of lambs, where changes in echocardiographic parameters are evident with advancing age.
Arbutin-Modified Microspheres Prevent Osteoarthritis Progression by Mobilizing Local Anti-Inflammatory and Antioxidant Responses
Authors: Jin Jiale , Liu Yang , Jiang Chao , Shen Yifan , Chu Guangyu , Liu Can , Jiang Lejian , Huang Guanrui , Qin Yifang , Zhang Yijian , Zhang Chi , Wang Yue 
Published: March 2022

Abstract
Osteoarthritis (OA), a common chronic degenerative disease, is considered the leading cause of disability worldwide. However, the current clinical treatments for OA are still unsatisfactory. Arbutin (ARB) is a major active ingredient of the Chinese medicinal herb cowberry leaf, which exerts potential antioxidant and anti-inflammatory activity. Based on a microfluid platform, we fabricated ARB-loaded gelatine methacryloyl-Liposome (GM-Lipo@ARB) microspheres that showed long-term release and excellent cartilage-targeting effects. The ARB-loaded microspheres effectively reduced the inflammatory response in interleukin (IL)-1β-treated arthritic chondrocytes. Moreover, GM-Lipo@ARB microspheres regulated cartilage extracellular matrix (ECM) homeostasis by upregulating nuclear factor erythroid 2-related factor 2 (Nrf2)-associated antioxidant properties. Intraarticular injection of GM-Lipo@ARB microspheres alleviated cartilage erosion in the destabilization of the medial meniscus (DMM)-mediated mouse OA model. Our results indicated that ARB-modified microspheres prevented cartilage degeneration by not only inhibiting the inflammatory reaction but also mitigating oxidative stress. This study proposes a novel ARB-laden functional microsphere for treating OA through the mobilization of local anti-inflammatory and antioxidant responses.
Self-rotating string loop’s overall state in vacuum and microgravity
Authors: Ma Hongzhi , Lan Ding , Zhai Sihan 
Published: March 2022

Abstract
A flexible string loop possesses a certain degree of rigidity when it moves. Based on this intriguing property, overall states of a string loop when it self-rotates driven by friction and when the whole is moved by a control end in vacuum and microgravity are investigated theoretically. When the string loop self-rotates, tension is uniformly distributed along the longitudinal direction of the string. At the same time, the shape is unknown under the current theoretical framework. When the string loop is moved as a whole, an expression of lateral stiffness is proposed, and limit conditions of translation acceleration, rotational angular velocity and rotational included angle that require to be imposed to control lateral force and deformation are put forward. This research is conducive to the development of control technology of string loops, and further is hopeful to be applied to projects such as debris capture and tethered satellites in space.
Bioplastic Design using Multitask Deep Neural Networks
Authors: Kuenneth Christopher , Lalonde Jessica , Marrone Babetta , Iverson Carl , Ramprasad Rampi , Pilania Ghanshyam 
Published: March 2022

Abstract
Non-degradable plastic waste stays for decades on land and in water, jeopardizing our environment; yet our modern lifestyle and current technologies are impossible to sustain without plastics. Bio-synthesized and biodegradable alternatives such as the polymer family of polyhydroxyalkanoates (PHAs) have the potential to replace large portions of the world's plastic supply with cradle-to-cradle materials, but their chemical complexity and diversity limit traditional resource-intensive experimentation. In this work, we develop multitask deep neural network property predictors using available experimental data for a diverse set of nearly 23000 homo- and copolymer chemistries. Using the predictors, we identify 14 PHA-based bioplastics from a search space of almost 1.4 million candidates which could serve as potential replacements for seven petroleum-based commodity plastics that account for 75% of the world's yearly plastic production. We discuss possible synthesis routes for these identified promising materials. The developed multitask polymer property predictors are made available as a part of the Polymer Genome project at https://PolymerGenome.org.
KORHN risk score for early neuroprognostication in cardiac arrest patients
Authors: Choi Wook Jin , Lee Jae Hoon , Jang Tae Chang , Oh Sang Hoon , Im Jee Yong 
Published: March 2022

Abstract
Background:Early prediction of poor neurologic outcomes in patients who have recovered spontaneous circulation from out-of-hospital cardiac arrest (OHCA) is the important process that can help family relatives choose a course of treatment. This study aimed to create a scoring tool that could predict an early poor prognosis of comatose survivors from OHCA upon admission and to compare the prognostic value with existing models (MIRACLE, TTM, CAHP, C-GRApH, and OHCA scores).Methods:Using a cohort from the Korean Hypothermia Network (KORHN) registry in Korea, numerous variables including basic demographics, resuscitation, and post-resuscitation were extracted from 1,371 out of 10,258 OHCA patients. Variables for prognostication were analyzed, and the KORHN risk score was developed from the adjustment of scores through cubic spline plots. The performance of the score was compared with five staple risk scores.Results:Eight variables for predicting a poor neurologic outcome were identified: low flow time, heart rate/diastolic arterial pressure, noncardiac cause, unreactive pupil reflex, nonshockable rhythm, GCS motor, epinephrine use, and age (adjusted odds ratios 4.287, 3.523, 3.438, 3.393, 3.087, 2.614, 2.526, and 2.419, respectively). The KORHN score showed higher area under the curve values (0.925 and 0.914) than previous risk scores with whole variables (0.827–0.902) and the leading five variables (0.85–0.903).Conclusions:The KORHN score is a simple tool for the neuroprognostication of OHCA patients during admission. This study confirmed that the KORHN score is a good alternative to the existing scoring models in predicting poor neurological outcomes from OHCA patients.Trial registration:KORHN-PRO, NCT02827422. Registered 11 September 2016 – Retrospectively registered.
Changes in the mental wellbeing among Nigerians due to Ramadan intermittent fasting: A nationwide cross-sectional study
Authors: Sulaiman Sahabi Kabir , Tsiga-Ahmed Fatimah Isma’il , Arora Teresa , Faris MoezAlIslam E , Musa Muhammad Sale , Kareem Yesir Adeyemi , Dayyab Farouq Muhammad , Hussein Aminu , Sale Shehu , Khan Moien AB 
Published: March 2022

Abstract
This study examined mental wellbeing and associated factors among Nigerian adults who observed Ramadan intermittent fasting (RIF) amid the COVID-19 pandemic. A cross-sectional online study was conducted among 770 adult Nigerians who observed RIF. Using pre-tested, web-based questionnaires, data about mental wellbeing (depression, anxiety), spirituality, and intrinsic religiosity were collected using validated generalised anxiety disorder-2 (GAD-2) and Patient Health Questionnaire-2 (PHQ-2), four-item spiritual wellbeing index (4-ISWBI) and the Muslim intrinsic religiosity questionnaire. Respondents' mental wellbeing before and during Ramadan was compared. The factors associated with the feeling of depression and anxiety were determined using multinomial regression analysis. When compared to mental wellbeing prior to Ramadan, observing RIF by Nigerian adults were associated with improved mental wellbeing.
Single Nucleotide Mutation Changes the Capability of CCN3 in Osteosarcoma Cell Invasion and Differentiation
Authors: Yan Xuejing , Fu Xin , Xu Yuqiao , Zhu Ning , Qiu Chuan , Bu Mengmeng , Shen Yan , Yan Wei , Chen Meihong 
Published: March 2022

Abstract
Background: This study is designed to identify the significant mutations in CCN3 gene in osteosarcoma and explore its influence in cell invasion and differentiation and the underlying mechanism. Methods: Sanger sequencing was applied to identify CCN3 gene sequence in human osteosarcoma cell lines, peripheral blood mononuclear cells (PBMC) and osteosarcoma tissue. Wildtype and mutant CCN3 (mCCN3) were ectopically expressed by lentivirus in human osteosarcoma cell lines. Tumor cell invasion was measured by Trans-well assay. Osteogenic differentiation was induced by osteogenic differentiating medium, and evaluated by alkaline phosphatase (ALP) activity, collagen type I alpha 1 chain (COL1A1) and osteocalcin expression. Western blotting was used to detect the protein level of CCN3 and mCCN3 in cytoplasmic, nuclear and secreted fractions of the cells. Results: A G to A single nucleotide mutation in coding region of CCN3 was found in both osteosarcoma cell and tissue. The frequency of this mutation in osteosarcoma tissue was much higher than that in para-carcinoma tissue and PBMC of healthy people. This nucleotide mutation decreased the nuclear glycosylated protein level of CCN3, and affected osteosarcoma cell invasion and differentiation. The lower nuclear ratio of glycosylated/non-glycosylated isoforms and the function change accounted for the different behavior of mCCN3 from that of CCN3. Conclusion: The G to A mutation identified in CCN3 resulted in differential glycosylated full length protein level and altered the function role of CCN3 in osteosarcoma cell invasion and differentiation.