bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gravity-Vector Induces Mechanical Remodeling of rMSCs via Combined Substrate Stiffness and Orientation
Authors: Zhang Chen , Lü Dongyuan , Zhang Fan , Wu Yi , Zheng Lu , Zhang Xiaoyu , Li Zhan , Sun Shujin , Long Mian 
Published: February 2022

Distinct physical factors originating from the cellular microenvironment are crucial to the biological homeostasis of stem cells. While substrate stiffness and orientation are known to regulate the mechanical remodeling and fate decision of mesenchymal stem cells (MSCs) separately, it remains unclear how the two factors are combined to manipulate their mechanical stability under gravity vector. Here we quantified these combined effects by placing rat MSCs onto stiffness-varied poly-dimethylsiloxane (PDMS) substrates in upward (180°), downward (0°), or edge-on (90°) orientation. Compared with those values onto glass coverslip, the nuclear longitudinal translocation, due to the density difference between the nucleus and the cytosol, was found to be lower at 0° for 24 h and higher at 90° for 24 and 72 h onto 2.5 MPa PDMS substrate. At 0°, the cell was mechanically supported by remarkably reduced actin and dramatically enhanced vimentin expression. At 90°, both enhanced actin and vimentin expression worked cooperatively to maintain cell stability. Specifically, perinuclear actin stress fibers with a large number, low anisotropy, and visible perinuclear vimentin cords were formed onto 2.5 MPa PDMS at 90° for 72 h, supporting the orientation difference in nuclear translocation and global cytoskeleton expression. This orientation dependence tended to disappear onto softer PDMS, presenting distinctive features in nuclear translocation and cytoskeletal structures. Moreover, cellular morphology and focal adhesion were mainly affected by substrate stiffness, yielding a time course of increased spreading area at 24 h but decreased area at 72 h with a decrease of stiffness. Mechanistically, the cell tended to be stabilized onto these PDMS substrates via β1 integrin–focal adhesion complexes–actin mechanosensitive axis. These results provided an insight in understanding the combination of substrate stiffness and orientation in defining the mechanical stability of rMSCs.
A Nonsense Variant of ZNF462 Gene Associated With Weiss–Kruszka Syndrome–Like Manifestations: A Case Study and Literature Review
Authors: Zhao Shaozhi , Miao Chen , Wang Xiaolei , Lu Yitong , Liu Hongwei , Zhang Xinwen 
Published: February 2022

Objective: This study aims to explore the clinical characteristics and genetic basis of a patient with unilateral ptosis and unilateral hearing impairment in pedigree analysis.Methods: The clinical data of the child and his father were collected. The genomic DNA of the patient and his relatives were extracted from their peripheral blood samples and subjected to trio-whole-exome sequencing (trio-WES) and copy number variation analysis. Sanger sequencing was used to verify the potential variant.Results: The sequencing analysis identified a heterozygous nonsense variant c.6431C &gt; A (p.Ser2144*) in the ZNF462 gene (NM_021224.6) in the child and his father, whereas the locus in his asymptomatic mother, brother, and grandparents was found to be the wild type, which is an autosomal dominant inheritance. The new genetic variant has not been previously reported in the ClinVar and HGMD databases and the Genome Aggregation Database (gnomAD).Conclusion: This is the first incidence of Weiss–Kruszka syndrome relating to the nonsense variant in the ZNF462 gene in China. The finding from this study is novel in its expansion of the variant spectrum of the ZNF462 gene and clarifies the genetic etiology of the patient and his father.
The Serious Challenge of Occult Hepatitis B Virus Infection-Related Hepatocellular Carcinoma in China
Authors: Xia Renxiang , Peng Jing , He Jian , Jiang Ping , Yuan Chunyan , Liu Xiaoli , Yao Yunqing 
Published: February 2022

BackgroundIt is unknown how many people in China have chronic occult hepatitis B virus (HBV) infection (OBI) [chronic HBV infection with negative serum hepatitis B surface antigen (HBsAg) (N-HBsAg)]. Their clinical and virological characteristics, especially the correlation between the OBI and hepatocellular carcinoma (HCC), are still elusive and need to be investigated, including prevention, early diagnosis, and treatment strategies.Methods138 patients with HCC related to OBI were screened from 698 patients of HCC associated with HBV infection, their characteristics of epidemiology, clinical, biochemistry, virology, diagnostics, and therapeutics were analyzed retrospectively. Furthermore, the correlation between virological features and clinical features was investigated.ResultsIt was found that 19.8% (138/698) of patients with HBV-related HCC were OBI, of which 79.7% (110/138) were men, and 20.3% (28/138) were women. Most of the patients with OBI-related HCC were older men, and the median age was 63.2 years. In total 78.3% (108/138) of the patients had apparent right upper abdomen discomfort and/or pain and then sought medical examination, while 21.7% (30/138) of the patients were identified by health examination. A total of 10.9% (15/138) of the patients were admitted with chronic infection of HBV, and 2.2% (3/138) of the patients were admitted with a family history of hepatitis B. The alpha-fetoprotein (AFP) serum-positive rate was 39.1% (54/138). Tumor lesions &gt;5.0 cm, with intrahepatic and/or extrahepatic metastasis, were found in 72.5% (100/138) of the patients. The diameter of the tumor in the Group of hepatitis B core antibody-positive [HBcAb(+)] and hepatitis B surface antibody-positive [HBsAb(+)] was 7.03 ± 3.76 cm, which was much smaller than 8.79 ± 4.96 cm in the Group of HBcAb(+) and HBsAb(−) (P = 0.035).ConclusionIt is estimated that at least 21 million OBI patients live in China. HBcAb(+) was not only the evidence of chronic HBV infection but also a dangerous mark for surface antigen-negative patients. A semi-annual or annual medical checkup is essential for all OBI patients to identify HCC as early as possible. The hypothesis underlying our analysis was that hepatitis B surface antibody would prevent the progress of HCC and facilitate the clearance of HBV in patients with OBI. Thereby, the hepatitis B vaccine could be used to prevent severe disease consequences.
Validation of the Chinese Version of the Stigma Scale of Epilepsy
Authors: Wu Yuanxia , Huang Kailing , Wen Shirui , Xiao Bo , Feng Li 
Published: February 2022

PurposeThis study was carried out to test the validity and reliability of the Chinese version of the Stigma Scale of Epilepsy (SSE), with aim to better understand the public stigmatizing attitudes of epilepsy in China and help elucidate stigma determinants for interventions.MethodsThe SSE was translated into Simplified Chinese Mandarin. In this study, most of the participants were enrolled via convenience sampling by randomly distributing questionnaires on the streets and parts of the participants were recruited by an online platform named Wenjuanxing. We assessed the psychometric properties of the SSE in 310 Chinese native-speaker. Cronbach's alpha was tested for reliability. Index of Content Validity (CVI) was calculated. Exploratory and confirmatory analysis were used to explore the factor structure and verify the validity of SSE.ResultsThe Cronbach's alpha is 0.936 for the overall scale, and the CVI value is greater than 0.78. The exploratory factor analysis (EFA) extracted SSE six factors: the fear of seizure attacks (factor 1), sympathy for patients with epilepsy (PWEs) (factor 2); difficulties faced by PWEs (factor 3); speculation on PWEs' feeling (factor 4); discrimination against PWEs (factor 5); and knowledge about epilepsy (factor 6). The item 13 was proven to be problematic and has been eliminated. The confirmatory factor analysis (CFA) ensured the great construct validity (χ2/SD = 1.725, goodness of fit index (GFI) = 0.916, and root mean square error of approximation (RMSEA) = 0.048), convergent validity (the factor loads of each item corresponding to each latent variable &gt;0.6, average variance extracted (AVE) &gt; 0.5, and composite reliability (CR) &gt; 0.7), and discrimination validity (all of the absolute value of correlation coefficient are &lt;0.5,and less than the square root of AVE) of the SSE.ConclusionsThe Chinese version of the SSE scale was a valid and reliable tool to measure epilepsy-associated stigma in the Chinese society.
Safety and Long-Term Efficacy Outcomes for Endovascular Treatment of Wide-Neck Bifurcation Aneurysms of the Middle Cerebral Artery: Insights From the SMART Registry
Authors: De Leacy Reade , Bageac Devin V. , Siddiqui Neha , Bellon Richard J. , Park Min S. , Schirmer Clemens M. , Woodward Keith B. , Zaidat Osama O. , Spiotta Alejandro M. 
Published: February 2022

IntroductionWide-necked middle cerebral artery bifurcation aneurysms pose specific challenges to endovascular management. Surgical clipping remains the standard treatment approach for these aneurysms in many centers. While recent data suggests the endovascular treatment may be comparable, a prospective datapoint has been lacking.Materials and MethodsThe Penumbra SMART registry, a prospective, multi-center, single-arm outcomes registry of Penumbra coil-treated aneurysms, was queried for endovascularly treated MCA bifurcation aneurysms with wide necks (dome:neck ratio &lt;2 or neck &gt;4 mm). Safety and efficacy outcomes were summarized for ruptured and unruptured aneurysms, including rupture, complication rate, and 1-year occlusion outcomes.ResultsSeventy-two aneurysms across 31 sites were enrolled. Of these, a total of 15 presented as ruptured aneurysms. Serious adverse events were reported in 21 (29.2%) of patients, with 8 (11.1%) attributed to the device/procedure. Immediately postoperatively, 75.0% of cases achieved “adequate” Raymond Roy Class I (40.3%) or II (34.7%) occlusion outcomes. Of the 72 patients treated, 60 (83.3%) underwent follow-up angiography at 1 year, and among these, 95.0% had 1-year occlusion outcomes of Raymond Roy Class I (71.7%) or II (23.3%). A total of 6 aneurysms (10.0%) were required or were planned for retreatment at the last follow-up.ConclusionThis study represents the most significant prospective sample of endovascularly treated wide-neck MCA bifurcation aneurysms conducted to date. It supports the safety and efficacy of endovascular treatment of these aneurysms.
Enterocolitis Is a Risk Factor for Bowel Perforation in Neonates With Hirschsprung's Disease: A Retrospective Multicenter Study
Authors: Zhu Tianqi , Zhang Guofeng , Meng Xinyao , Yang Jixin , Niu Yonghua , He Ying , Yang Heying , Xiong Xiaofeng , Feng Jiexiong 
Published: February 2022

Background and AimWe evaluated the clinical features of neonatal Hirschsprung's disease (HD)-associated bowel perforation (perforated HD) and investigated risk factors related to it.MethodsWe retrospectively collected clinical data of neonates (&lt;1 month of age) with perforated HD from multicenters in China from January 2006 to December 2019. A total of 142 patients (6.7%) with perforated HD were enrolled in the study. A 1:2 matching method was used to compare the clinical information of HD patients with and without bowel perforation during the neonatal period. The risk factors for bowel perforation were identified using univariate and multivariate logistic risk regression analyses.ResultsPerforation site was present in the proximal ganglionic bowel in 101 (71.1%) cases and the distal aganglionosis segment in 41 (28.9%) cases. Adjacent marginal tissue from the perforated intestine revealed varying degrees of inflammatory cell infiltration, and the severity of enterocolitis was higher in the proximal ganglionic bowel than in the distal aganglionosis segment (p &lt; 0.05). In the univariable and multivariable logistic analyses, clinical symptoms, such as vomiting (adjusted OR = 2.06, 95% CI: 2.01–2.88, p &lt; 0.05), and inflammation index in hematologic tests, such as neutrophil proportion (adjusted OR = 1.09, 95% CI: 1.05–1.33, p &lt; 0.05) and CRP (adjusted OR = 2.13, 95% CI: 1.01–3.27, p &lt; 0.05) were associated with increased risk for perforated HD.ConclusionClinical Hirschsprung disease-associated enterocolitis (HAEC) highly correlated with perforated HD. Timely treatment of HAEC should be appropriate therapeutic approaches to prevent perforated HD.
The Application of Technological Intervention for Stroke Rehabilitation in Southeast Asia: A Scoping Review With Stakeholders' Consultation
Authors: Selamat Siti Nur Suhaidah , Che Me Rosalam , Ahmad Ainuddin Husna , Salim Mazatulfazura S. F. , Ramli Hafiz Rashidi , Romli Muhammad Hibatullah 
Published: February 2022

BackgroundThe technological intervention is considered as an adjunct to the conventional therapies applied in the rehabilitation session. In most high-income countries, technology has been widely used in assisting stroke survivors to undergo their treatments. However, technology use is still lacking in Southeast Asia, especially in middle- and low-income countries. This scoping review identifies and summarizes the technologies and related gaps available in Southeast Asia pertaining to stroke rehabilitation.MethodsThe JBI manual for evidence synthesis was used to conduct a scoping study. Until September 2021, an electronic search was performed using four databases (Medline, CINAHL, Scopus, ASEAN Citation Index). Only the studies that were carried out in Southeast Asia were chosen.ResultsForty-one articles were chosen in the final review from 6,873 articles found during the initial search. Most of the studies reported the implementation of technological intervention combined with conventional therapies in stroke rehabilitation. Advanced and simple technologies were found such as robotics, virtual reality, telerehabilitation, motion capture, assistive devices, and mobility training from Singapore, Thailand, Malaysia, and Indonesia. The majority of the studies show that technological interventions can enhance the recovery period of stroke survivors. The consultation session suggested that the technological interventions should facilitate the needs of the survivors, caregivers, and practitioners during the rehabilitation.ConclusionsThe integration of technology into conventional therapies has shown a positive outcome and show significant improvement during stroke recovery. Future studies are recommended to investigate the potential of home-based technological intervention and lower extremities.
EFEKTIVITAS OBAT KUMUR EKSTRAK ETANOL DAUN BELUNTAS (Pluchea indica L. ) UNTUK MENGATASI HALITOSIS PADA REMAJA
Authors: Sumerti Ni Nengah , Tedjasulaksana Regina , Nahak Maria Martina 
Published: February 2022

Known as bad breath, halitosis also called oral malodor is a bad breath that comes from air expelled by a person through the mouth. Halitosis is caused by local and systemic factors, but 90% of patients with halitosis due to local factors in the oral cavity, including at adolescents. The purpose of this study was to determine the effectiveness of mouthwashes of ethanol extract of leaves beluntas to overcome halitosis. This is an experimental research with completely randomized with pre-post control group design. Samples are adolescents junior high school students. Data were analyzed by Wilcoxon test, Results shown that there is a significance difference before and after rinsing in the groups and Friedman test shown that there is the significance difference between groups. The results of the study shown that mean average of mouth rinsing with ethanol extract of beluntas leaves with 12.5% in concentration is 0.167 ± 0.408, with p = 0.038 or p 0.05, rinsing with ethanol extract of beluntas leaves with 25% in concentration is 0.516 ± 0.408 , with p = 0.023 or p 0.05, mouth rinsing using chlorhexidine 0,12% shown that mean is 0.000 ± 0.000, with p = 0.020 or p 0.05. mouth rinsing using Base formula material shown that mean is 0,833 ± 0.408, with p = 0,157 or p 0.05. Analyze differences between groups to know the efectiveness of mouthwash treatment with Friedman test shown that p value = 0.000 or p0.05. The conclusion is rinsing with ethanol extract of beluntas leaves with 12.5% and 25% in concentration and chlorhexidine 0,12% can reduce halitosis significantly, but rinsing with base formula of mouthwash can not be overcome halitosis significantly. There are significant differences of effectiveness of mouthwash between groups to overcome halitosis. Keywords: mouthwash, ethanol extract of beluntas leaves, halitosis, adolescent
PENGARUH MEDIA BOOKLET TERHADAP PENINGKATAN PENGETAHUAN, SIKAP DAN PERILAKU PASIEN DIABETES MELLITUS DALAM PENCEGAHAN KELAINAN PERIODONTAL DI PUSKESMAS II DENPASAR UTARA TAHUN 2018
Authors: Agung Anak Agung Gede , Wirata I Nyoman , Arini Ni Wayan 
Published: February 2022

Periodontal disorders is one of the risks that can occur at patients with Diabetes Mellitus. The ignorance of patients for prevention and checking their teeth and mouth that caused periodontitis and result in early tooth lossed. The one solution that can be done for Diabetes Mellitus patients in preventing of periodontal disorders is to right brush their teeth regularly. The aim of the study was to analyze the influence of booklet to increase of knowledge, attitude, and behaviour of Diabetes Mellitus patients in prevention of periodontal diseases in prevention of periodontal diseases at Puskesmas II Denpasar Utara in 2018. The type of this study is action research that was in dental health education form to improve the knowledge and behaviour to maintain the healthy teeth to total population that amount to 100 patients with Diabetes Mellitus in Puskesmas II Denpasar Utara. The results of this study were based on Paired Sample Test and Willcoxon showed that there is a significant effect on using the booklet to increase of knowledge, attitudes, and behavior of tooth brushing that each p = 0.00 (p 0.05).
Pengetahuan Personal Hygiene Berhubungan Dengan Timbulnya Gejala Penyakit Kulit Pada Petugas Pengangkut Sampah
Authors: Nahrajanti Putu Firda Ika , Sudiadnyana I Wayan 
Published: February 2022

Knowledge of personal hygiene is very important to note by the garbage collector to maintain their life , health and welfare in prevent symptoms of skin diseases. This research to find out the knowledge of the personal hygiene with the symptoms of a skin disease in the garbage collector. This type research is analytic survey on the interview with the cross sectional.The sample as many as 70 officers garbage, located in the Badung Regency. Data were collected by using a questionnaire and statistical analysis by using the Chi Square test. The result from the garbage collector knowledge are about 12,9% have a good knowledge, about 32,8% have an averange knowledge and about 54,3% have a bad knowledge. Respondents with some symptoms as much as 58,6 % and some who were not as symptoms is as much as 41.1 % . Symptoms of skin disease most often happen by the garbage collector were itchy, red stain, redness of the skin , bumps, scaly skin, and peeling skin. Chi Square statistical results P value = 0,000 ɑ (0.05), which means there is a significant relationship between the level of knowledge about personal hygiene with the onset of symptoms of skin diseases in the garbage collector with a moderate level of relationship (CC value = 0.537). The garbage collector should further enhance his knowledge of personal hygiene by given counseling.
Bienestar psicológico y desempeño docente en la educación básica regular
Authors: Vargas Navarro Rachel , Idrogo Barboza Gloria , Aguirre Idrogo Yesica Mabel , Noel Bravo Gina Paola 
Published: February 2022

La presente investigación tuvo como objetivo, establecer la relación entre el bienestar psicológico y el desempeño de los docentes, en una institución educativa del cono Sur de Lima, 2019. Para esto, se utilizó una metodología con enfoque cuantitativo, de tipo básico. El diseño de investigación fue no experimental, correlacional y de corte transversal. La población estuvo conformada por 90 docentes y la muestra fue censal. Se utilizó la técnica de la encuesta y como instrumentos, fueron utilizados dos cuestionarios, los cuales fueron validados por juicio de expertos y demostrado su confiabilidad, mediante una prueba piloto realizada a 30 docentes. Según el análisis de resultados, se concluye que con respecto a la variable bienestar psicológico y desempeño docente, que existe una correlación positiva y moderada entre las variables, ya que se obtuvo el Rho ,575, y la significancia de p-valor = ,000 que es menor a ?= ,05 por lo cual se rechaza la hipótesis nula.
Gestión directiva y el trabajo con la comunidad durante la emergencia sanitaria por el COVID-19
Authors: Ruesta Quiroz Ruth Giovanna , Gejaño Ramos Cindy Victoria , Vega Vilca Carlos Sixto 
Published: February 2022

El año 2020 en Perú se declaró la emergencia sanitaria por el COVID-19, esta coyuntura exigió que desde la gestión directiva se implementar acciones para garantizar la continuidad de la educación en colaboración con la comunidad, conformada por los padres de familia, por esta razón se formuló como objetivo explorar desde la literatura académica la gestión directiva y el trabajo con la comunidad durante la emergencia sanitaria por el COVID-19, se empleó el enfoque cualitativo de revisión bibliográfica, de la revisión de la literatura se concluyó que, las dificultadas en Perú fueron similares a las de otros países, en los cuales desde la dirección se debieron generar espacios de conexión, también se evidenció afecciones emocionales en los padres, quienes tuvieron dificultades en ayudar a sus hijos en esta nueva normalidad.
El aporte de la pragmática y el sentido del contexto en la comprensión de textos
Authors: Deza Sánchez Maximina Angélica , Manzanares Gómez Jaqueline Jéssica , Huamán Ocaña Carmen Madaleyne , Guerrero Huamán Fredy Esmit 
Published: February 2022

El estudio tiene como objetivo analizar el aporte de la pragmática y el sentido del contexto en la comprensión de textos con la finalidad de caracterizar la naturaleza del texto considerando dos ideas: la comunicación no convencional y la naturaleza del texto como insumo para PISA. Se ha utilizado el método narrativo-biográfico en tanto los resultados desfavorables caracterizan las limitaciones de escolares peruanos al encarar la prueba PISA 2018. Se ha valorado la narrativa existente de los resultados a través del análisis documental en cuanto a los aportes de la pragmática y su funcionalidad. Los resultados cualitativos reflejan que la prueba PISA 2018 no considera el contexto como insumo para seleccionar los textos para evaluar la comprensión. A modo de conclusión, los resultados obtenidos en la prueba PISA 2018 no reflejan el nivel de comprensión de los escolares.