bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distributive and collaborative push‐and‐pull in an artificial microbial consortium for improved consolidated bioprocessing
Authors: Fang Hao , Deng Yuntao , Pan Yingjie , Li Chaofeng , Yu Liang 
Published: August 2022

AbstractThe consolidated bioprocessing (CBP) of lignocellulose by the synthetic microbial consortium of Trichoderma reesei and Saccharomyces cerevisiae is a promising way of biomanufacturing d‐glucaric acid. However, the hindrance factor to its industrial application is the low efficiency. Therefore, we engineered T. reesei and S. cerevisiae to improve the CBP for d‐glucaric acid production. T. reesei was engineered to produce more cellulase and release more fermentable sugars from lignocellulose, that is, pushing more sugars to S. cerevisiae. S. cerevisiae was engineered to metabolize cellobiose and siphon more sugars into d‐glucaric acid biosynthetic pathway, that is, pulling more sugars to S. cerevisiae. This is the strategy of distributive and collaborative push‐and‐pull we developed and proposed in this work, which was proven successful in improving efficiencies of the CBPs of steam‐exploded corn stover (SECS) for d‐glucaric acid production and distiller's grains for single cell protein (SCP) production. The titer, yield and productivity of d‐glucaric acid produced from 50 g/L SECS by the microbial consortium of T. reesei C10 and S. cerevisiae LGA‐1C3S2 were 6.42 g/L, 0.128 g/g SECS, and 0.917 g/L/d, respectively. The titer, yield, and productivity of SCP produced from 80 g/L distiller's grains were 50.5 g/L, 0.631 g/g distiller's grains, and 8.417 g/L/d, respectively. These were much higher than the initial microbial consortia of T. reesei Rut‐C30 and S. cerevisiae LGA‐1 or INVSc1. The results confirmed the applicability and generalizability of distributive and collaborative push‐and‐pull, which has profound meaning for science and engineering.
“Don't let anybody ever put you down culturally…. it's not good…”: Creating spaces for Blak women's healing
Authors: Balla Paola , Jackson Karen , Quayle Amy F , Sonn Christopher C , Price Rowena K 
Published: August 2022

AbstractResearch has highlighted the importance of Indigenous knowledge and cultural practice in healing from ongoing histories of trauma, dispossession, and displacement for Indigenous peoples in Australia and elsewhere. Connection with culture, Country, and kinship has been identified as protective factors for Aboriginal social and emotional well‐being and as facilitating cultural healing. This paper draws on stories mediated through cultural practice specifically, Wayapa and bush‐dyeing workshops, to explore how women resignified experiences and engaged in “healing work.” Our collaborative analysis of the stories shared resulted in three main themes that capture dialogs about the need for culturally safe spaces, vulnerability and identity, and culture, Country, and place. Centering Aboriginal knowledge, our analysis shows the meanings of Country, spirituality, and the coconstitution of people, culture, and the natural environment. Through Indigenous cultural practice, the women “grew strength in relationship” as they engaged in the psychosocial processes of deconstruction, reclamation, and renarrating personal and cultural identities.
Toward Sustainable Photo‐/Electrocatalytic Carboxylation of Organic Substrates with CO2
Authors: Nandi Shantanu , Jana Ranjan 
Published: August 2022

AbstractHistorically, carbon dioxide found many applications in organic chemistry from reaction medium e. g., super critical carbon dioxide (scCO2) or CO2‐expanded liquid (CXL) system; extraction (natural products), separation (supercritical fluid chromatography); chemo‐, regio‐, and stereoselective transformation via the formation of transient intermediates i. e., carbonate, carbamate etc. Recently, researchers have exploited CO2 as an inexpensive, renewable and non‐toxic C1 feedstock for carboxylation and carbonylation reactions. However, owing to its inherent kinetic and thermodynamic stability, most of the transformations require high temperatures, high pressure, and stoichiometric amounts of organometallic reductants that leads to accidental and environmental hazards. Inspired by the natural processes, photo‐ and electrocatalytic conversion of CO2 into value‐added products has emerged as a benign and practical approach in organic synthesis. Here in this review, we will discuss some recent photo‐ and electrocatalytic methods and their comparison toward sustainable development.
Design and Synthesis of Fluoro‐containing Hypervalent Iodane (III) Reagents for Visible‐light‐triggered Cyclization of Alkynoates to 3‐Fluoroalkylated Coumarins
Authors: Zhou Ningning , Wang Kun , Zhao Tingting , Liu Ruiyue , Rui Mingyang , Zhao Xia , Feng Jiaxu , Lu Kui 
Published: August 2022

AbstractA series of novel fluoro‐containing hypervalent iodane (III) reagents was designed and synthesized. By employing these novel hypervalent iodane (III) reagents, an efficient visible‐light‐triggered radical cyclization of alkynoates to diversely 3‐fluoroalkylated coumarins was developed under metal free condition. It provided an alternative and environmental friendly approach for the installation of 3‐fluoroalkyl motif into synthetically valuable building block coumarins.
Novel score for predicting early emergency endovascular therapy in trauma care using logistic LASSO regression
Authors: Yokoyama Taro , Nakahara Shinji , Kondo Hiroshi , Miyake Yasufumi , Sakamoto Tetsuya 
Published: August 2022

AimTo support decision‐making for early interventional radiology, this study aimed to derive and validate a novel and simple scoring system for predicting the necessity of interventional radiology therapies in trauma patients.MethodsThis retrospective study used data derived from the medical records of patients with severe traumatic injuries treated at a tertiary‐level emergency institution. The score was derived from 168 patients treated between April 2015 and October 2016 and validated using data from 68 patients treated between November 2016 and July 2017. Logistic “least absolute shrinkage and selection operator (LASSO)” regression was used to select predictors. In order to compose the score, odds ratios derived from the logistic model were simplified to integer score coefficients. The score was evaluated using the area under the receiver operating characteristic curve. The best cut‐off point for the score was determined using Youden’s index, and sensitivity and specificity were calculated.ResultsThe derived score comprised three predictors (systolic blood pressure, positive findings in abdominal ultrasound assessment, and pelvic fracture) and ranged from 0 to 30. On validation, the area under the receiver operating characteristic curve for the score was 0.86 (95% confidence interval, 0.64–1.00). The sensitivity and specificity were 80% and 89%, respectively, with a cut‐off point of 3.ConclusionThis simple score, requiring variables obtainable immediately after hospital arrival, could aid in facilitating early interventional radiology team activation.
Super‐Coordinated Nickel N4Ni1O2Site Single‐Atom Catalyst for Selective H2O2Electrosynthesis at High Current Densities
Authors: Xiao Chuqian , Cheng Ling , Zhu Yihua , Wang Gengchao , Chen Luyang , Wang Yating , Chen Rongzhen , Li Yuhang , Li Chunzhong 
Published: August 2022

AbstractElectrochemical production of hydrogen peroxide (H2O2) from O2on single‐atom catalysts has attracted great attention, yet the quest for robust catalysts is driven by achieving >90 % Faradaic efficiency (FE) under industrial‐relevant current densities (>100 mA cm−2). Herein we synthesize a catalyst that contains single nickel site coordinated by four nitrogen and two oxygen atoms (i.e., N4Ni1O2) via involving carboxyl functionalized multiwall carbon nanotubes as a substrate to provide extra O coordination to the regular NiN4site. It has a cathodic energy efficiency of approximately 82 % and a H2O2FE of around 96 % at 200 mA cm−2current density, outperforming the reported single‐atom catalysts for H2O2electrosynthesis.
Diastereoselective, Catalytic Access to Cross‐Aldol Products Directly from Esters and Lactones
Authors: Moreno González Adrián , Nicholson Kieran , Llopis Natalia , Nichol Gary S. , Langer Thomas , Baeza Alejandro , Thomas Stephen P. 
Published: August 2022

AbstractHigh oxidation‐state carbonyl coupling partners including esters and lactones were reacted with enones to give aldol‐type products directly using two‐fold organoborane catalysis. This new retrosynthetic disconnection to aldol‐type products is compatible with enolisable coupling partners, without self‐condensation, and couples the high reactivity of secondary dialkylboranes with the stability of pinacolboronic esters. Excellent chemoselectivity, substrate scope (including those containing reducible functionalities and free alcohols) and diastereocontrol were achieved to access both the syn‐ and anti‐aldol‐type products. Mechanistic studies confirmed the two‐fold catalytic role of the single secondary borane catalyst for boron enolate formation and formation of an aldehyde surrogate from the ester or lactone coupling partner.
Peri‐operative refractory anaphylactic shock during hepatic hydatid cyst surgery
Authors: Gundappa P. , Prakash D. , Palankar N. , Karanth S. 
Published: August 2022

SummarySerious intra‐operative anaphylactic shock is an unusual complication. Identification of causative agents can be difficult due to the number of anaesthetic drugs administered in quick succession, many of which can potentially cause anaphylaxis. Most anaphylactic reactions respond to, and resolve with, adrenaline and steroids. However, they can be prolonged and life‐threatening. Refractory anaphylaxis, unresponsive to repeated doses of adrenaline, is rare and associated with a high risk of mortality. Rupture of a hydatid cyst during surgery can cause anaphylaxis. Here, we present a rare case of refractory anaphylactic shock during surgical resection of a hepatic hydatid cyst, with severe haemodynamic instability requiring prolonged postoperative ventilation and triple inotropic support for seven days in the intensive care unit. We describe the management of this condition, primarily based on steroid therapy and escalating vasopressor support, particularly where limited response to adrenaline is encountered. Refractory anaphylaxis is a rare but life‐threatening complication of hydatid cyst surgery. Therefore, anaphylactic reactions should always be anticipated, with the appropriate treatment and facilities readily available.
Synthesis and property study of novel maleimide‐cardanol based modifiers for epoxy resins
Authors: Chen Mengyu , Liu Licheng , Wang Gang , Liu Zhuoyu , Yu Yingfeng 
Published: August 2022

AbstractThe biomass‐based chemicals from renewable resources are countermeasures to ease the problem of fossil fuel shortage. This work is to develop a biobased functional reactive modifier for thermosetting epoxy resins appropriate for excellent overall performance. The novel maleimide‐cardanol modifiers (CA‐HPM) is synthesized from natural extract cardanol (CA) and N‐(4‐hydroxyphenyl) maleimide (HPM), which is then mixed with phenolic epoxy resin and linear phenolic novolac resin systems. CA‐HPM plays a versatile role in tuning the comprehensive properties of epoxy such as thermal property, adhesive property, and thermo‐mechanical property. Systems with CA‐HPM show slightly lower curing activity and distinct decreased single glass transition temperature (Tg). Besides, the crosslinking density of the cured resin increases, meanwhile the thermal stability keeps at high level, which is due to the rigid five‐membered ring structure of maleimide. Metal lap shear test results manifest that the adhesion between epoxy resin and metal interface augments. With 10% CA‐1HPM, the cured resin exhibits lower equilibrium water sorption (1.88%) and diffusion coefficient (2.94 × 10−8 s−1 cm2). This biobased functional reactive modifier has provided balanced overall performance and would pave the way to a wide range of possible “eco‐friendly” applications.
Structure, properties, and in vitro degradation behavior of biodegradable poly(L‐lactic acid)‐trimethylene carbonate‐glycolide terpolymer
Authors: Xia Qi , Ye Wuyou , Zhang Qin , Liu Dongyang , Gao Shida , Fan Zhongyong , Liu Qing 
Published: August 2022

AbstractFor biodegradable medical implants, it is critical to match the degradation rate of the material with the lesion healing rate of the tissue defect. Here, we report the synthesis of poly(L‐lactic acid) (PLLA)‐trimethylene carbonate (TMC)‐glycolide (GA) terpolymers with various monomer feeding ratios of L‐lactide, TMC, and GA. The terpolymers were prepared through ring‐opening polymerization with the purpose to improve degradation and mechanical properties of the terpolymers for biomedical applications. The influence of various GA contents in PLLA‐TMC‐GA terpolymers on the chain structure and their in vitro degradation performances were evaluated by using gel permeation chromatography, differential scanning calorimetry, X‐ray diffraction, 1H nuclear magnetic resonance, and tensile tests. Compared with pure PLLA, the degradation rate of terpolymers increased with the increase of GA content, because GA segments, which have a faster hydrolysis rate, not only effectively disrupt the regularity of LLA segment, but also increase the probability of chains scission during the degradation process. In a 28‐week long degradation study, the mass loss of PLLA‐TMC‐GA terpolymers was about 36%, which is a faster rate than reported for pure PLLA. Therefore, it is possible to tailor the copolymer chain structures by varying the ratio of GA in the terpolymer to balance its degradation rate with body's lesion healing rate.
Synthesis, Structure and Studies of Meso‐Triaryl 22‐Oxabenzonorroles
Authors: Grover Vratta , Pushpanandan Poornenth , Ravikanth Mangalampalli 
Published: August 2022

AbstractThe first examples of meso‐triaryl 22‐oxabenzonorroles were synthesized in 18–21% yields by oxidation of appropriate meso‐triaryl oxabenzobilane. The key precursors, the meso‐triaryl oxabenzobilanes were synthesized readily by treating 16‐oxatripyrrane with (1H‐indol‐3‐yl)(p‐tolyl)methanol under acid catalyzed conditions. The meso‐triaryl 22‐oxabenzonorroles were obtained in free base form by following our simple straight‐forward synthetic strategy. The X‐ray structure obtained for meso‐tritolyl 22‐oxabenzonorroles revealed that the indole ring was deviated by an angle of 41.27° from the mean plane. The NMR studies supported the presence of diatropic ring current effect as inner NH and CH protons were observed in the upfield region. The absorption spectra of 22‐oxabenzonorroles exhibited one strong Soret band and four weak Q‐bands. The electrochemical studies revealed that the macrocycles are electron rich and undergo easier oxidations. The DFT and TD‐DFT studies were in agreement with the experimental observations and NICS and HOMA values supports the aromatic nature of meso‐triaryl oxabenzonorroles.
Sulfur quantum dots as a novel platform to design a sensitive chemiluminescence probe and its application for Pb2+ detection
Authors: Mojarrad Sima , Naseri Abdolhossein , Hallaj Tooba 
Published: August 2022

AbstractThe monitoring of Pb as a hazardous heavy metal element for the environment and human health is of high importance. In this study, a simple and sensitive chemiluminescence (CL) probe based on sulfur quantum dots (SQDs) was designed for the determination of Pb2+. To the best of our knowledge, this is the first report on the analytical application of the CL method based on SQDs. For this purpose, SQDs were synthesized using a simple hydrothermal method and characterized using transmission electron microscopy, Fourier transform infrared spectroscopy, X‐ray photoelectron spectroscopy and X‐ray diffraction. Then, the direct CL of SQDs elicited by common oxidants was investigated. The highest CL intensity was observed for the SQDs–KMnO4 reaction, and its CL mechanism was studied. We indicated that the CL intensity of introduced system can be diminished as a result of the interaction between Pb2+ and SQDs, and exploited this fact for designing a CL‐based probe for the determination of Pb2+. The CL intensity of the SQDs–KMnO4 reaction was linearly quenched using Pb2+ in the range 50–2000 nM with a limit of detection of 16 nM (S/N = 3). The probe was used for the determination of Pb2+ in different water samples and the recovery results (95.2–102.8%) indicated the good analytical performance of the developed method.
Quantification of 3‐bromopyruvate in rat plasma by HPLC–MS/MS employing precolumn derivatization and the application to a pharmacokinetics study
Authors: Liu Hui , Zhang Qi , Huang Hesong , Qiu Mingliang , Hu Lvjiang , Zhao Xiaojuan , Xu Chuanxi , Wang Zhiqiang , Zhang Chenlei , Zhan Yang , Sun Yongbing 
Published: August 2022

AbstractA simple high‐performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method has been developed for the determination of 3‐bromopyruvate (3‐BrPA) in rat plasma for the first time. The analytes were separated on a C18 column (100 × 2.1 mm, 1.7 μm) and a triple‐quadrupole mass spectrometry equipped with an electrospray ionization source was applied for detection. 3‐BrPA was extracted from rat plasma with protein precipitation, and then derivatized with 4‐nitro‐1,2‐phenylenediamine to obtain the mass signal because 3‐BrPA could not be detected in mass spectrometry. The method was fully validated according to the US Food and Drug Administration guidance. The method was linear over the concentration range 0.5–1,000.0 ng/ml for 3‐BrPA. The precision and accuracy, extraction recovery, and matrix effect were within acceptable limits. The method was then applied to support a pharmacokinetic study after 3‐BrPA and 3‐BrPA‐l‐Val‐l‐Ile (a dipeptide prodrug of 3‐BrPA, 3‐BrPA‐l‐Valine‐l‐isoleucine) had been administered to the Sprague–Dawley rats, respectively.
A rare presentation of lung squamous cell carcinoma metastasis to the distal phalanx of the little finger: a case report
Authors: Wu Jianjun , Zhou Yinan , Yang Dawei , Yang Fan 
Published: August 2022

AbstractLung cancer is one of the most common malignant tumors worldwide. Early detection is the most important factor that affects the prognosis of lung cancer patients. The distal phalanx is a highly unusual metastatic site of lung cancer, and is often misdiagnosed, especially when accompanied by trauma. Herein, we present the case of a 68‐year‐old male patient who injured his right little finger. We hope this rare case will help clinicians to better diagnosis acral metastases of lung cancer to prolong patient survival.
Attitudes of patients with cutaneous melanoma toward prognostic testing using the 31‐gene expression profile test
Authors: Ahmed Kelli , Siegel Jennifer J. , Morgan‐Linnell Sonia K. , LiPira Kyleigh 
Published: August 2022

AbstractObjectiveAlthough most patients diagnosed with early‐stage cutaneous melanoma (CM) have excellent outcomes, because of the large number diagnosed each year, many will experience recurrence or death. Prognostic testing for CM using the 31‐gene expression profile (31‐GEP) test can benefit patients by helping guide risk‐appropriate treatment and surveillance plans. We sought to evaluate patients' attitudes toward prognostic testing with the 31‐GEP and assess whether patients experience decision regret about having 31‐GEP testing.MethodsA 43‐question survey was distributed by the Melanoma Research Foundation in June–August 2021 to CM patients enrolled in their database. Patients were asked questions regarding their decision to undergo 31‐GEP testing and the extent to which they experienced decision regret using a validated set of Decision Regret Scale questions.ResultsWe analyzed responses from patients diagnosed in 2014 or later (n = 120). Of these, 28 had received 31‐GEP testing. Most respondents (n = 108, 90%) desired prognostic information when diagnosed. Of those who received 31‐GEP testing, most felt the results were useful (n = 22 out of 24) and had regret scores significantly less than neutral regret, regardless of their test results (Class 1: p < 0.001; Class 2: p = 0.036). Further, decision regret scores were not significantly different between patients who received a Class 1 31‐GEP result and those who received a Class 2 result (mean Class 1 = 1.39 and mean Class 2 = 1.90, p = 0.058).ConclusionsMost newly diagnosed CM patients desired prognostic information about their tumors. Patients who received 31‐GEP testing felt it was useful and did not regret their decision to undergo 31‐GEP testing.
Cerebral embolic protection and severity of stroke following transcatheter aortic valve replacement
Authors: Isogai Toshiaki , Vanguru Husitha Reddy , Krishnaswamy Amar , Agrawal Ankit , Spilias Nikolaos , Shekhar Shashank , Saad Anas M. , Verma Beni Rai , Puri Rishi , Reed Grant W. , Popović Zoran B. , Unai Shinya , Yun James J. , Uchino Ken , Kapadia Samir R. 
Published: August 2022

AbstractBackgroundThe cerebral embolic protection (CEP) device captures embolic debris during transcatheter aortic valve replacement (TAVR). However, the impact of CEP on stroke severity following TAVR remains unclear. Therefore, we aimed to examine whether CEP was associated with reduced severity of stroke following TAVR.MethodsThis was a retrospective cohort study of 2839 consecutive patients (mean age: 79.2 ± 9.5 years, females: 41.5%) who underwent transfemoral TAVR at our institution between 2013 and 2020. We categorized patients into Sentinel CEP users and nonusers. Neuroimaging data were reviewed and the final diagnosis of a cerebrovascular event was adjudicated by a neurologist blinded to the CEP use or nonuse. We compared the incidence and severity (assessed by the National Institutes of Health Stroke Scale [NIHSS]) of stroke through 72 h post‐TAVR or discharge between the two groups using stabilized inverse probability of treatment weighting (IPTW) of propensity scores.ResultsOf the eligible patients, 1802 (63.5%) received CEP during TAVR and 1037 (36.5%) did not. After adjustment for patient characteristics by stabilized IPTW, the rate of overall stroke was numerically lower in CEP users than in CEP nonusers, but the difference did not reach statistical significance (0.49% vs. 1.18%, p = 0.064). However, CEP users had significantly lower rates of moderate‐or‐severe stroke (NIHSS ≥ 6: 0.11% vs. 0.69%, p = 0.013) and severe stroke (NIHSS ≥ 15: 0% vs. 0.29%, p = 0.046). Stroke following CEP use (n = 8), compared with stroke following CEP nonuse (n = 15), tended to carry a lower NIHSS (median [IQR], 4.0 [2.0–7.0] vs. 7.0 [4.5–19.0], p = 0.087). Four (26.7%) out of 15 patients with stroke following CEP nonuse died within 30 days, with no death after stroke following CEP use.ConclusionsCEP use may be associated with attenuated severity of stroke despite no significant difference in overall stroke incidence compared with CEP nonuse. This finding is considered hypothesis‐generating and needs to be confirmed in large prospective studies.