bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expresión y producción de la lacasa POXA 1B recombinante de pleurotus ostreatus como alternativa para el tratamiento de los subproductos líquidos que se obtienen en el proceso de blanqueamiento de pulpa de celulosa
Authors:   , Rivera Hoyos Claudia Marcela 
Published: July 2022

Las lacasas están generando un gran interés debido a sus potenciales aplicaciones en biotecnología. Son oxidasas multicobre que catalizan compuestos aromáticos y no-aromáticos, con la subsecuente reducción del oxígeno molecular a agua. En el presente trabajo se seleccionó el gen codificante para la lacasa POXA IB del hongo Pleurotos ostreatos. Este gen se optimizó para el uso de codones en Pichia pastons, contenido de GC y regiones que generen estructuras secundarias. Se evaluó la interacción del modelo computacional obtenido y su interacción con el sustrato ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-ácido sulfónico)] realizando un docking molecular. Luego de realizar un diseño experimental Plackett-Burman, seguido de un diseño One Factor, se obtuvo una actividad lacasa de 1,343.52 ± 40.3 UL'1 a las 168h de cultivo en frascos de 500mL. El sobrenadante concentrado conteniendo la enzima POXA IB mantuvo su estabilidad entre 10-50 °C y retuvo más del 70% de la actividad enzimática residual a 60°C y del 50% a 70°C. Adicionalmente, la enzima fue más estable a pH 4 ± 0.2 con una actividad residual de más del 90%. La actividad residual más baja del 60% se obtuvo a pH 10 ± 0.2. Adicionalmente, los parámetros cinéticos fueron Vmax de 3.163 x 10'2 mM s"1, y Km de 1.720 mM. La decoloración del licor sintético a los 5 días de tratamiento fue de 19, 53 y 59%, para las concentraciones de licor de 10, 5 y 1% (v/v), y la remoción de DQO de 5, 30 y 53%, respectivamente, con actividades lacasa de 1399, 1346 y 1327 UL'1 para cada una de las concentraciones evaluadas. Toda esta información reunida, puede considerarse como hallazgos importantes a la luz de la expresión de enzimas recombinantes para su uso en sistemas ambientalmente amigables, debido al amplio rango de sustratos que pueden ser transformados por las lacasas. Contribuyendo a una gran gama de productos en diversos entornos: la industria, el uso clínico y química y aplicaciones ambientales.
Cartografía social como recurso metodológico en los procesos de planeación participativa de un territorio incluyente : el caso del plan parcial de renovación urbana "El triángulo de fenicia" en la ciudad de Bogotá
Authors:   , Castro Jaramillo María Lucía 
Published: July 2022

El presente documento es el informe final del estudio que tuvo como objetivo principal encontrar evidencias de la aplicación de cartografía social en un caso de estudio para establecer, que, como metodología, permite incluir y traducir las diferentes visiones de la comunidad en planes y diseños del territorio. Dicho ejercicio se realizó a partir de la revisión y documentación de un estudio de caso significativo de la ciudad de Bogotá: el plan parcial de renovación urbana El Triángulo Fenicia", en el periodo del 2007 - 2014. El estudio de caso elegido fue un caso de interés para el propósito de esta investigación, teniendo en cuenta que fue un proyecto que en su concepción y formalización utilizó la cartografía social como metodología participativa. Cabe resaltar que este plan, adhiriéndose a los objetivos generales del Plan Zonal del Centro , tuvo como lineamiento principal la creación de un territorio incluyente, a través de la promoción de la participación ciudadana. El alcance de esta investigación consistió en establecer, para el caso colombiano, la relevancia de la participación ciudadana frente a los procesos de planeación y diseño del territorio, evidenciando la cartografía social una metodología que facilita la participación ciudadana en dichos procesos, para finalmente identificar los posibles criterios para la construcción de indicadores aplicables al seguimiento de un proceso de planeación participativa.
The effects of the -Tú eliges- program on the demand for student loans in Colombia
Authors:   , Alvarez Velasco Juan Carlos , Vallejo Franco Mauricio 
Published: July 2022

Este proyecto analiza los efectos del programa "tú eliges" en la demanda de préstamos estudiantiles en Colombia. En este sentido caracterizamos la demanda de estos préstamos y examinamos los efectos de "tú eliges" en el nivel de aspiración de los solicitantes, en cuanto a los montos de préstamos solicitados y logros académicos. Utilizando los datos de las solicitudes de crédito de ICETEX, que van desde el semestre 2014-II hasta 2016-I y estimando las regresiones por MCO, examinamos las respuestas de la demanda a la implementación del programa. Los resultados muestran aumentos del 3,6% y 4,2% en los montos de los préstamos solicitados después de implementar el programa. Además, confirman la relevancia de ingresos y estrato en el consumo educativo. Los solicitantes de "tú eliges", presentan logros académicos mayores para los puntajes de Saber 11° (+21.7 puntos) al solicitar un préstamo estudiantil.
Cuantificación de las diferencias dosimétricas generadas por la selección del algoritmo de cálculo en un sistema de planeación de radioterapia
Authors:   , Valdivieso Cárdenas Julián Augusto 
Published: July 2022

Los algoritmos de "Pencil Beam", y "Collapse Cone" empleados en el sistema de planeación de tratamiento Oncentra Master Plan, son usados para el cálculo de la dosis sobre una matriz tridimensional (imágenes del paciente), implementando la técnica de radioterapia por intensidad modulada IMRT en casos de cáncer de próstata. Puesto que las soluciones para tal cálculo son análisis fisicomatemáticos distintos del mismo problema, se puede esperar que los resultados obtenidos sean diferentes. El presente trabajo se ha dedicado a la búsqueda de tales diferencias con una herramienta computacional propia que analiza las matrices y cuantifica dichas diferencias mediante el método de análisis gamma, y el cálculo de la probabilidad de complicaciones en tejido normal NTCP.
Recital de grado de maestría en Interpretación, violín - Juan David Hernandez
Authors:   , Hernández Benavides Juan David , Kagehira Mari 
Published: July 2022

Programa

Johann Sebastian Bach (1685-1750)
Sonata n.º 2 en la menor BWV 1003
- Grave
- Fuga

Claude Debussy (1862-1918)
Sonata para violín y piano en Sol menor
-	Allegro vivo
-	Intermède: Fantasque et léger
-	Finale: Très animé

Johannes Brahms (1833-1897)
Concierto para violín en Re mayor Op. 77
-	Allegro non troppo
-	Adagio
- Allegro giocoso, ma non troppo vivace - Poco più presto
mundo a través de las palabras
Authors: Marqués Pascual Joaquín 
Published: July 2022

Es esta una obra colectiva surgida del amalgamiento de varias disciplinas: el poder del lenguaje, la justicia como defensa de las colectividades estructuradas, y la comunicación como una forma de desarrollo crítico de la comunidad. Esta compilación de diversos conocimientos genera un libro donde el eje central se concentra en una revisión de la categoría del género y sus identidades.
Ready for impact? A validity and feasibility study of instrumented mouthguards (iMGs)
Authors: Jones Ben , Tooby James , Weaving Dan , Till Kevin , Owen Cameron , Begonia Mark , Stokes Keith A , Rowson Steven , Phillips Gemma , Hendricks Sharief , Falvey Éanna Cian , Al-Dawoud Marwan , Tierney Gregory 
Published: July 2022

Objectives
Assess the validity and feasibility of current instrumented mouthguards (iMGs) and associated systems.

Methods

Phase I; four iMG systems (Biocore-Football Research Inc (FRI), HitIQ, ORB, Prevent) were compared against dummy headform laboratory criterion standards (25, 50, 75, 100
g
). Phase II; four iMG systems were evaluated for on-field validity of iMG-triggered events against video-verification to determine true-positives, false-positives and false-negatives (20±9 player matches per iMG). Phase III; four iMG systems were evaluated by 18 rugby players, for perceptions of
fit
,
comfort
and
function
. Phase IV; three iMG systems (Biocore-FRI, HitIQ, Prevent) were evaluated for practical feasibility (System Usability Scale (SUS)) by four practitioners.

Results

Phase I; total concordance correlation coefficients were 0.986, 0.965, 0.525 and 0.984 for Biocore-FRI, HitIQ, ORB and Prevent. Phase II; different on-field kinematics were observed between iMGs. Positive predictive values were 0.98, 0.90, 0.53 and 0.94 for Biocore-FRI, HitIQ, ORB and Prevent. Sensitivity values were 0.51, 0.40, 0.71 and 0.75 for Biocore-FRI, HitIQ, ORB and Prevent. Phase III; player perceptions of
fit
,
comfort
and
function
were 77%, 6/10, 55% for Biocore-FRI, 88%, 8/10, 61% for HitIQ, 65%, 5/10, 43% for ORB and 85%, 8/10, 67% for Prevent. Phase IV; SUS (preparation-management) was 51.3–50.6/100, 71.3–78.8/100 and 83.8–80.0/100 for Biocore-FRI, HitIQ and Prevent.

Conclusion
This study shows differences between current iMG systems exist. Sporting organisations can use these findings when evaluating which iMG system is most appropriate to monitor head acceleration events in athletes, supporting player welfare initiatives related to concussion and head acceleration exposure.
Clinical code usage in UK general practice: a cohort study exploring 18 conditions over 14 years
Authors: Zghebi Salwa S , Reeves David , Grigoroglou Christos , McMillan Brian , Ashcroft Darren M , Parisi Rosa , Kontopantelis Evangelos 
Published: July 2022

ObjectiveTo assess the diagnostic Read code usage for 18 conditions by examining their frequency and diversity in UK primary care between 2000 and 2013.DesignPopulation-based cohort studySetting684 UK general practices contributing data to the Clinical Practice Research Datalink (CPRD) GOLD.ParticipantsPatients with clinical codes for at least one of asthma, chronic obstructive pulmonary disease, diabetes, hypertension (HT), coronary heart disease, atrial fibrillation (AF), heart failure, stroke, hypothyroidism, chronic kidney disease, learning disability (LD), depression, dementia, epilepsy, severe mental illness (SMI), osteoarthritis, osteoporosis and cancer.Primary and secondary outcome measuresFor the frequency ranking of clinical codes, canonical correlation analysis was applied to correlations of clinical code usage of 1, 3 and 5 years. Three measures of diversity (Shannon entropy index of diversity, richness and evenness) were used to quantify changes in incident and total clinical codes.ResultsOverall, all examined conditions, except LD, showed positive monotonic correlation. HT, hypothyroidism, osteoarthritis and SMI codes’ usage had high 5-year correlation. The codes’ usage diversity remained stable overall throughout the study period. Cancer, diabetes and SMI had the highest richness (code lists need time to define) unlike AF, hypothyroidism and LD. SMI (high richness) and hypothyroidism (low richness) can last for 5 years, whereas cancer and diabetes (high richness) and LD (low richness) only last for 2 years.ConclusionsThis is an under-reported research area and the findings suggest the codes’ usage diversity for most conditions remained overall stable throughout the study period. Generated mental health code lists can last for a long time unlike cardiometabolic conditions and cancer. Adopting more consistent and less diverse coding would help improve data quality in primary care. Future research is needed following the transfer to the Systematised Nomenclature of Medicine Clinical Terms (SNOMED CT) coding.
Modelling and in vivo evaluation of tendon forces and strain in dynamic rehabilitation exercises: a scoping review
Authors: Escriche-Escuder Adrian , Cuesta-Vargas Antonio I , Casaña Jose 
Published: July 2022

ObjectivesAlthough exercise is considered the preferred approach for tendinopathies, the actual load that acts on the tendon in loading programmes is usually unknown. The objective of this study was to review the techniques that have been applied in vivo to estimate the forces and strain that act on the human tendon in dynamic exercises used during rehabilitation.DesignScoping review.Data sourcesEmbase, PubMed, Web of Science and Google Scholar were searched from database inception to February 2021.Eligibility criteriaCross-sectional studies available in English or Spanish language were included if they focused on evaluating the forces or strain of human tendons in vivo during dynamic exercises. Studies were excluded if they did not evaluate tendon forces or strain; if they evaluated running, walking, jumping, landing or no dynamic exercise at all; and if they were conference proceedings or book chapters.Data extraction and synthesisData extracted included year of publication, study setting, study population characteristics, technique used and exercises evaluated. The studies were grouped by the types of techniques and the tendon location.ResultsTwenty-one studies were included. Fourteen studies used an indirect methodology based on inverse dynamics, nine of them in the Achilles and five in the patellar tendon. Six studies implemented force transducers for measuring tendon forces in open carpal tunnel release surgery patients. One study applied an optic fibre technique to detect forces in the patellar tendon. Four studies measured strain using ultrasound-based techniques.ConclusionsThere is a predominant use of inverse dynamics, but force transducers, optic fibre and estimations from strain data are also used. Although these tools may be used to make general estimates of tendon forces and strains, the invasiveness of some methods and the loss of immediacy of others make it difficult to provide immediate feedback to the individuals.
Evaluation of the Desire to Avoid Pregnancy Scale in the UK: a psychometric analysis including predictive validity
Authors: Hall Jennifer , Barrett Geraldine , Rocca Corinne 
Published: July 2022

ObjectivesTo evaluate the psychometric performance, including predictive validity, of a UK version of the Desire to Avoid Pregnancy (DAP) scale.DesignProspective cohort study for psychometric evaluation.SettingUK.ParticipantsWomen in the UK aged 15 years to menopause, who were not pregnant at the time of recruitment in October 2018, were eligible. 994 women completed the baseline survey and 90.2% of women eligible for the 12-month survey participated.Primary and secondary outcome measuresThe DAP scale was assessed according to key measurement properties of validity (construct (structural and hypothesis testing) and criterion (predictive)), reliability (internal consistency using Cronbach’s alpha and test–retest using intraclass correlation coefficients, ICC) and differential item functioning. Item response and classical test theory methods were used.ResultsThe scale was acceptable, understandable and showed good targeting with the full range of scores captured. Construct validity was demonstrated on hypothesis testing, with odds of contraceptive use increasing threefold with each increasing DAP point (range: 0–4). Eighty per cent of women with the lowest DAP score became pregnant within 12 months, compared with <1% of those with the highest DAP score. Reliability, both in terms of internal consistency (Cronbach’s α 0.96) and test–retest (ICC 0.95), was excellent. Some tests of structural validity, in relation to model fit with the item-response model, were not met, and investigations suggest further exploration of the factor structure of the DAP is required in other samples. Item 5, regarding relationship with a partner, showed differential item functioning by age, number of children and relationship group.ConclusionsThe UK DAP is a valid and reliable measure of women’s DAP and is highly predictive of pregnancy within the next 12 months. Further evaluations should continue the assessment of the factor structure and the performance of the item relating to the partner.
Parametric and non-parametric estimation of reference intervals for routine laboratory tests: an analysis of health check-up data for 260 889 young men in the South Korean military
Authors: Kim Taeyun , Choi Hyunji , Lee Sun Min 
Published: July 2022

ObjectivesDetermination of reference intervals (RIs) using big data faces several obstacles due to heterogeneity in analysers, period and ethnicity. The present study aimed to establish the RIs for routine common blood count (CBC) and biochemistry laboratory tests in homogeneous, healthy, male Korean soldiers in their 20s using a large health check-up data set, comparing parametric and non-parametric estimation.DesignA multicentre, cross-sectional study.SettingSeven armed forces hospitals in South Korea.ParticipantsA total of 609 649 men underwent health examination when promoted to corporal between January 2015 and September 2021. 260 889 eligible individuals aged 20–25 were included in the analysis.Main outcomes and measuresThe RIs were established by parametric and non-parametric methods. In the parametric approach, maximum likelihood estimation was applied to measure the Box-Cox transformation parameter and the values at the 2.5th and 97.5th percentiles were recalculated. The non-parametric approach adopted the Tukey’s exclusion test and the values at the 2.5th and 97.5th percentiles were obtained. Classification by body mass index was also performed.ResultsThe obtained RIs for haematology parameters were comparable between devices. If the values followed a Gaussian distribution, parametric and non-parametric methods were well matched for haematology and biochemical markers. When the values were right-skewed, the upper limits were higher with parametric than with non-parametric methods. Participants with obesity showed higher RIs for CBC, some liver function tests and some lipid profiles than participants without obesity.ConclusionsUsing data from healthy, male Korean soldiers in their 20s, we proposed the RIs for CBC and biochemical parameters, comparing parametric and non-parametric estimation. As such approaches based on large data sets become more prevalent, further studies are needed to discriminate eligible individuals and determine RIs in an extrapolated sample.
Thyroid-stimulating hormone and mortality in pulmonary arterial hypertension
Authors: Pi Hongyang , Rayner Samuel G , Ralph David D , Nolley Stephanie , Barros Lia M , Steinberg Zachary L , Leary Peter J 
Published: July 2022

IntroductionPulmonary arterial hypertension (PAH) remains a serious and life-threatening illness. Thyroid dysfunction is relatively understudied in individuals with PAH but is known to affect cardiac function and vascular tone in other diseases. The aim of this observational study was to evaluate the association between thyroid-stimulating hormone (TSH), mortal and non-mortal outcomes in individuals with PAH.MethodsThe Seattle Right Ventricle Translational Science (Servetus) Study is an observational cohort that enrolled participants with PAH between 2014 and 2016 and then followed them for 3 years. TSH was measured irrespective of a clinical suspicion of thyroid disease for all participants in the cohort. Linear regression was used to estimate the relationships between TSH and right ventricular basal diameter, tricuspid annular plane systolic excursion and 6-minute walk distance. Logistic regression was used to estimate the relationship with New York Heart Association Functional Class, and Cox proportional hazards were used to estimate the relationship with mortality. Staged models included unadjusted models and models accounting for age, sex at birth and aetiology of pulmonary hypertension with or without further adjustment for N-terminal-pro hormone brain natriuretic peptide.ResultsAmong 112 participants with PAH, TSH was strongly associated with mortality irrespective of adjustment. There was no clear consistent association between TSH and other markers of severity in a cohort with PAH.DiscussionThis report reinforces the important observation that TSH is associated with survival in patients with PAH, and future study of thyroid dysfunction as a potential remediable contributor to mortality in PAH is warranted.
Development and test–retest reliability of a new, self-report questionnaire assessing healthcare use and personal costs in people with inflammatory bowel disease: the Inflammatory Bowel Disease Resource Use Questionnaire (IBD-RUQ)
Authors: Roukas Chris , Syred Jonathan , Gordeev Vladimir Sergeevich , Norton Christine , Hart Ailsa , Mihaylova Borislava 
Published: July 2022

Background and aimsThe increasing prevalence of inflammatory bowel disease (IBD) poses a substantial economic burden globally on health systems and societies. Validated instruments to collect data on healthcare and other service utilisation by patients with IBD are lacking. We developed a self-report patient questionnaire to capture key resource utilisation from health services, patient and societal perspectives.MethodsThe IBD Resource Use Questionnaire (IBD-RUQ), developed by a multidisciplinary team, including patients, comprises 102 items across the six categories of outpatient visits, diagnostics, medication, hospitalisations, employment and out-of-pocket expenses over the past three months. The test–retest reliability of the IBD-RUQ was studied by administering it twice among patients with IBD with a 2-week time gap. The intraclass correlation coefficients and the average cost from the healthcare, societal and patient perspectives, between test and retest assessments, overall and by service category, were summarised.ResultsThe IBD-RUQ captures health service use, employment and out-of-pocket expenses. Of 55 patients who completed the first questionnaire, 48 completed the retest questionnaires and were included in the analyses. Test–retest reliability for categories of medications, diagnostics, specialist outpatient and inpatient services, and days off work due to IBD ranged from moderate to excellent; primary care visits showed more limited reliability. The annualised average self-reported health service, out-of-pocket and loss of productivity costs were £4844, £320 and £545 per patient, respectively.ConclusionsThe IBD-RUQ is a reliable and valid self-report measure of resource utilisation in adults with IBD and can be used to measure costs associated with IBD.
Approach to laboratory ordering and interpretation in rheumatology
Authors: Miloslavsky Eli M 
Published: July 2022

Abstract
Evaluation of suspected rheumatic disease is a significant challenge due to overlapping and sometimes non-specific clinical features. Most laboratory tests in rheumatic disease have incomplete sensitivity and specificity, leading to positive results without disease and negative results despite disease presence. Therefore, judicious ordering and correct interpretation of laboratory testing in rheumatology is critical in order to provide high-value care. Herein we review laboratory testing in rheumatology in the context of a framework for approaching rheumatic disease.