bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forgerons à travers les générations ? La métallurgie dans l’îlot VI d’Olbia de Provence (Hyères, Var) de 325 av. J.-C. à 20 ap. J.-C
Authors: Pagès Gaspard , Roure Réjane , Salle Valérie 
Published: April 2022

La colonie massaliète d’Olbia de Provence (Hyères, Var) a récemment fait l’objet d’une campagne de fouilles programmées sous la direction de Michel Bats : l’îlot VI de la cité hellénistique a pu être intégralement étudié et a révélé une série d’ateliers de métallurgistes qui se succèdent dans des espaces différents mais au sein du même îlot, depuis sa fondation dans le dernier quart du ive siècle av. J.-C. jusqu’au milieu du ier siècle av. J.-C. Quatre ateliers ont livré des foyers, divers autres aménagements métallurgiques et des résidus liés à des activités de forge ; ils sont installés dans trois espaces domestiques différents, car ils se déplacent à chaque grande phase de restructuration de l’îlot. La présentation de ces différents ateliers et leur articulation avec les lieux de vie permet de souligner l’importance de la pérennité des espaces artisanaux et de leur implantation topographique.
IL-27 Induces CCL5 Production by T Lymphocytes, Which Contributes to Antitumor Activity
Authors: Hu Aiyan , Zhu Jianmin , Zeng Chunxi , Lin Cho-Hao , Yu Jianyu , Liu Jin-Qing , Lynch Kimberly , Talebian Fatemeh , Pan Xueliang , Yan Jingyue , Dong Yizhou , Li Zihai , Bai Xue-Feng 
Published: April 2022

Abstract
IL-27 is a pleiotropic cytokine that exhibits stimulatory/regulatory functions on multiple lineages of immune cells including T lymphocytes. In this study, we demonstrate that IL-27 directly induces CCL5 production by T lymphocytes, particularly CD8+ T cells in vitro and in vivo. IL-27–induced CCL5 production is IL-27R–dependent. In CD4+ T cells, IL-27–induced CCL5 production was primarily dependent on Stat1 activation, whereas in CD8+ T cells, Stat1 deficiency does not abrogate CCL5 induction. A chromatin immunoprecipitation assay revealed that in the CCL5 promoter region, both putative Stat3 binding sites exhibit significant binding to Stat3, whereas only one out of four Stat1 binding sites displays moderate binding to Stat1. In tumor-bearing mice, IL-27 induced dramatic production of CCL5 in tumor-infiltrating T cells. IL-27–induced CCL5 appears to contribute to an IL-27–mediated antitumor effect. This is signified by diminished tumor inhibition in anti-CCL5– and IL-27–treated mice. Additionally, intratumor delivery of CCL5 mRNA using lipid nanoparticles significantly inhibited tumor growth. Thus, IL-27 induces robust CCL5 production by T cells, which contributes to antitumor activity.
Loratadine vs Rupatadine
Authors: Fayaz Syed H , Varadarajan Sathyanarayanan , Ansari Sanofer , James Jerin 
Published: April 2022

Background:
Chronic Idiopathic Urticaria (CIU) is a debilitating disease characterised by almost daily presence of urticarial symptoms like short-lived wheals, itching, and erythema for at least 6 weeks without an identifiable cause there by leading to impairment of quality of life of the patient.

Aim:
To evaluate the efficacy and safety of loratadine and rupatadine in chronic idiopathic urticaria.

Methods:
This is a prospective, randomized, single-blind, parallel arm study conducted to evaluate the efficacy and safety of loratadine and rupatadine in patients with CIU. The study was registered prospectively with Clinical Trial registry of India (CTRI/2017/05/008624). Institutional Ethics Committee clearance was obtained. Written informed consent was obtained from all the participants before enrolment into the trial. The study was conducted in the outpatient department of Dermatology, SRM Medical College, Kattankulathur, Tamil Nadu, India, during the period from June 2017 to August 2018. Patients with CIU enrolled into the study based on inclusion-exclusion criteria were given the intervention drugs; Loratadine 10 mg once daily or rupatadine10 mg once daily orally for 6 weeks.

Results:
Rupatadine is more efficacious than loratadine in the reduction of Total Leucocyte Count, Differential Count and Absolute Eosinophil Count, the key determinants of allergy. Rupatadine also produced better improvement in Total symptom Score, Dermatology Life Quality Index in patients with CIU.

Conclusion:
Analysis of all the parameters of efficacy and safety establishes the probable superiority of rupatadine over loratadine for the treatment of urticaria.
A Cross-Sectional Evaluation of the Usefulness of the Minor Features of Hanifin and Rajka Diagnostic Criteria for the Diagnosis of Atopic Dermatitis in the Pediatric Population
Authors: Dutta Arnab , De Abhishek , Das Sudip , Banerjee Shyamal , Kar Chinmay , Dhar Sandipan 
Published: April 2022

Background:
Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disease. Hanifin and Rajka's criteria is the most common diagnostic criteria used for the clinical diagnosis of this condition. However, many find that it is too exhaustive to be used in routine practice, and the specificity of many of the minor criteria poses challenges, particularly from Asian countries with type III, IV, and V skin.

Aims and Objectives:
The aim of the study is to evaluate the effectiveness of the minor features of the Hanifin and Rajka criteria for AD in comparison to the UK working party's diagnostic criteria in pediatric populations of India.

Methodology:
A hospital-based cross-sectional study of 100 patients in the pediatric age group (3 months–12 years) with AD was conducted based on history, clinical, and laboratory evaluation. An age-matched control group of 100 pediatric patients who did not have a personal or family history of atopic dermatitis was included after obtaining informed consent to find out the prevalence of minor criteria among the control group.

Results:
Mean of the number of minor clinical criteria found positive in our study population in the infantile and toddler (below 2 years) and childhood groups (2–12 years) was (4.72 ± 1.75) and (5.67 ± 1.78), respectively. Early-onset of disease was the most consistent feature among the minor criteria found in 83% of patients, followed by xerosis (71%), hyperlinearity of palm (56%), pityriasis alba (54%), Denny Morgan fold (52%), elevated serum IgE (47%), perifollicular accentuation (37%), and tendency toward cutaneous infections (37%).

Conclusion:
We found that though some of the minor criteria are highly sensitive and specific to the diagnosis of AD (xerosis, ichthyosis, palmar hyperlinearity, tendency of cutaneous infections, Dennie–Morgan infraorbital fold, pityriasis alba, and perifollicular accentuation), some other criteria were either very rare or nonspecific for AD. We suggest that many of the minor criteria of Hanifin and Rajka may not have much significance for Indian patients and a multicentric nationwide study with a larger patient pool is required to create a trimmed and improved version of Hanifin and Rajka criteria.
Research on Hotair and 7SL-RNA Gene Expression Levels in Psoriasis Vulgaris
Authors: Yazıcı Seyma , Guner Rukiye Yasak , Akyol Melih , Tuzemen Bayyurt Elif Burcu , Arslan Serdal 
Published: April 2022

Backgrounds:
Mutation of protein-coding genes and non-coding genes is a factor in psoriasis etiology. Non-coding RNA (ncRNA), which does not have protein-coding capacity, is available in the human genome. HOTAIR (HOX Antisense Intergenic RNA) and 7SL-RNA are known as ncRNA. They may play a role in psoriasis pathogenesis.

Aims:
In our study, we aimed to investigate the level of HOTAIR and 7SL-RNA gene expression in the lesional and perilesional healthy skin of psoriasis patients.

Methods:
Total RNA isolation from the skin samples was achieved by modifying the RNeasy Mini Kit (Qiagen, Cat No: 74104) protocol. Real Time Polymerase Chain Reaction (qPCR) phase was performed in accordance with the protocol of the relevant brand (WizPure qPCR).

Results:
7SL-RNA gene expression decreased in the skin with psoriatic lesions (FC: 0.01; p: 0.028), and this decrease was statistically significant. HOTAIR gene expression decreased (FC: 0.92; p: 0.218), but this decrease was not statistically significant.

Conclusions:
lncRNAs may play a role in the pathogenesis of psoriasis disease.
Treatment of Plaque-Like Oral Lichen Planus with CO2 Laser
Authors: Dalirsani Zohreh , Seyyedi Seyyed Amir 
Published: April 2022

Background:
Oral lichen planus (OLP) is a chronic autoimmune disease, which annoys the patients for several years. It presents with various clinical forms. Although plaque-like form of OLP is usually asymptomatic, it could transform to a malignant lesion. Therefore, treatment considerations are essential to reduce the development of oral cancer. Laser therapy is a newer modality for treatment of plaque-like lesions.

Aims:
This study aimed to perform laser therapy for plaque-like lesions of OLP.

Methods:
We described some plaque-like OLP lesions evaporated with CO2 laser. The power of 4–7 watt as continuous wave (CW) and defocused mode was employed for evaporation. The complications and recurrence were evaluated in the follow-up sessions.

Results:
In some patients, mild keratotic lesions were observed in the follow-up sessions. Most patients were satisfied with treatment process and reported mild burning after treatment. No complication was observed in the patients.

Conclusion:
CO2 laser could be employed for OLP lesions; however, some degrees of recurrence may occur.
A Comparative Study on Therapeutic Efficacy of Autologous Platelet Rich Fibrin Matrix Versus Zinc Oxide And Phenytoin Paste in Non Healing Ulcers
Authors: Singh Akanksha , Chahar Yatendra S , Chhabra Shaiphali 
Published: April 2022

Context:
A non-healing ulcer occurs due to multiple causes and possesses a great impact on the quality of life. Autologous platelet-rich fibrin and the application of a triple combination paste (zinc oxide, phenytoin, and mupirocin ointment) have emerged with a good response in the treatment of non-healing ulcers.

Aims:
To compare the therapeutic efficacy of autologous platelet-rich fibrin matrix versus the triple combination paste (zinc oxide, phenytoin, and mupirocin ointment) in non-healing ulcers.

Settings and Design:
Hospital-based interventional comparative study.

Materials and Method:
Twenty-four patients with non-healing ulcers were randomly divided into two groups. One group received platelet-rich fibrin (PRF) therapy every week for up to 5 weeks and the second group applied triple combination paste (zinc oxide, phenytoin, and mupirocin ointment) again for 5 weeks. In the end, the ulcer area was measured.

Statistical Analysis Used:
Student's paired t-test was used to evaluate the correlations between variables. A P-value of < 0.05 was considered significant.

Results:
Group A showed a mean reduction in the ulcer area by 8.26 mm2 (75.99%). Group B showed a mean reduction in the ulcer area by 4.799 mm2 (47.75%), which was statistically significant with a P-value of 0.004.

Conclusion:
We conclude that autologous platelet-rich fibrin matrix is much more effective than the triple combination paste (zinc oxide, phenytoin, and mupirocin ointment) in the treatment of non-healing ulcers.
Development of a Rapid and Low-Cost Method for the Extraction of Dermatophyte DNA
Authors: Kenjar Apoorva , M Raj Juliet R , Bhandary Joshika , Girisha Banavasi S , Chakraborty Gunimala , Karunasagar Indrani 
Published: April 2022

Background:
Polymerase chain reaction (PCR) is the most optimized method for the rapid detection and analysis of any environmental or clinically significant organism. While PCR amplification directly from samples has been shown effective for several bacteria and viruses, for filamentous fungus and yeast, extraction of genomic DNA is a must. The extraction of DNA from fungal cultures is often reported using user-friendly commercially available kits, which are designed to decrease the time, extensive manual work in extraction procedures but are often expensive. Dermatophytes pose an added drawback to efficient DNA extraction due to their poor recovery on culture media and slow growth rate.

Aims and Objectives:
In the present study, we developed and validated a method for effective genomic DNA extraction from dermatophytes.

Materials and Methods:
DNA yield from standard dermatophytes extracted from spore suspensions and mycelia mat by commercially available kits was compared. A modified method using lyticase buffer and phenol-chloroform extraction was developed. The yield obtained was compared with the existing methods (kit-based method and cetyl trimethyl ammonium bromide method). The yield and quality of the total genomic DNA were estimated spectrophotometrically and by successful PCR amplification of the ITS region. The results were validated using 21 clinical isolates from recalcitrant dermatophytosis.

Results:
Minimal fungal DNA was obtained from the spores compared to that obtained from mycelial mat. Commercially available kits yielded lower amounts of DNA compared to the CATB method. The modified method developed in this study yielded better quality and quantity of DNA.

Conclusion:
Of the three extraction methods evaluated, the developed method gave significantly higher total genomic DNA yield and better purity than the reference methods. In addition, the turnaround time for DNA extraction was reduced to half based on modifications in culture conditions.
HPV Vaccination Status and Resolution of Warts in Pediatric Patients
Authors: Burli Anuk , Hancock Sarah , Zhao Yu Tina , Paul Deborah , Cordisco Maria 
Published: April 2022

Background:
Warts are a common dermatologic complaint with an increased incidence within the pediatric population. Warts are caused by multiple strains of the human papillomavirus (HPV). There is little research on how a patient's HPV immunization status affects the response to treatment of warts in pediatric patients.

Aims:
The purpose of this study is to investigate the relationship between HPV vaccination status and wart resolution.

Materials and Methods:
This is a retrospective chart review that investigates the relationship between response to routine treatment of warts and a subject's HPV vaccination status.

Results:
There was no significant relationship found between HPV vaccination status and resolution of warts (p = 0.797). However, there was a significant positive correlation between having the HPV vaccine and number of visits for the treatment of warts (r = 0.180, P = 0.024).

Conclusion:
This study did not show a significant correlation between HPV vaccination status and wart resolution, although it demonstrated a significant positive relationship between those immunized with the HPV vaccine and an increased number of treatment visits. Possible explanations for this unexpected correlation include the variation in HPV vaccine formulation, vaccination status, and frequency of office visits, since vaccinated patients are more likely to be compliant with office visits.
A Prospective Study Examining the Effect of Selected Topical and Systemic Drugs on Pruritus Grading System Score and STAT 6 Expression in Patients of Prurigo Nodularis
Authors: Agrawal Diksha , Sardana Kabir , Mathachan Sinu R , Bhardwaj Minakshi , Ahuja Arvind , Jain Swasti 
Published: April 2022

Background:
Prurigo nodularis (PN) is a chronic dermatologic condition presenting as multiple papulonodular lesions occurring with intense pruritus. Though numerous agents (topical, systemic, phototherapy and biological drugs) have been tried, the outcomes are variable.

Objectives:
The aim of this study was to assess the role of topical and systemic therapies in primary PN by comparing the Pruritus Grading System (PGS) score at baseline and 1 month post-therapy.

Materials and Methods:
Of 86 diagnosed cases of PN, 49 cases of primary PN were clinically graded by Pruritus Grading System Score (PGSS), and assessed histopathologically by IHC staining (STAT-1, 3, and 6). Apart from topical agents, oral nortriptyline (mild grade), methotrexate (moderate grade) and thalidomide (severe grade) were administered, whereas doxepin was administered for itching. The PGSS was assessed after 1 month of therapy.

Results:
Among 49 patients of PN, the majority of patients showed a significant decrease in PGSS (P = <0.001) in 1 mont, which correlated with STAT-6 expression. The combination of different topical and oral agents resulted in a statistically significant change in severity, though individual drugs did not achieve statistically significant results.

Conclusion:
A combination of selected oral and topical agents can effectively control the severity of PN within one month, and this was found to correlate with STAT 6 expression.
Clinical Characteristics of Patients with Chronic Urticaria and Adherence to Management Guidelines by Postgraduate Students in Dermatology
Authors: Reddy Sainath , Manchanda Isheeta , Godse Kiran , Patil Anant D 
Published: April 2022

Background:
Chronic urticaria is a heterogenous skin disorder representing one of the important reasons for consultation with a dermatologist. Dermatology post-graduate students play an importanrt role in the treatment of patients with chronic urticaria.

Objective:
The objective of the study was to describe clinical characteristics of patients with chronic urticaria and assess adherence to the guidelines by postgraduate students in the department of dermatology of a tertiary care center.

Materials and Methods:
In this retrospective study, prescriptions of patients with chronic urticaria and/or angioedema presenting to the outpatient department for 5 months were analyzed. Percentage of prescriptions adhering to international urticaria management guidelines was calculated. Urticaria Activity Score, percentage of patients receiving second-generation antihistamines, first-generation antihistamines, and other drugs was recorded. Comorbidities in patients with chronic urticaria were also noted.

Results:
A total of 60 patients (mean age 32.1 years; 58.3% male) were included in. Mean (SD) duration of urticaria at the time of study was 4.7 (2.7) months. Demographism and history of allergy to drugs was present in 45 (75%) and 4 (6.7%) patients. Mean (SD) Urticaria Activity Score was 12.5 (6.5). A total of 12 (20%) patients had comorbidities. Mean number of drugs received per patient was 1.7 (0.5). A total of 47 (78.3%) patients received second-generation antihistamines, whereas 11 (18.3%) received first-generation antihistamines. Two (3.3%) patients received combination of first-generation and second-generation antihistamines. Fexofenadine, levocetirizine, bilastine, and cetirizine was prescribed to 24 (40%), 26 (43.3%), 18 (30%), and 14 (23.3%) patients. There was no significant difference in male and female patients receiving fexofenadine (P = 0.59) or levocetirizine (P = 0.13).

Conclusion:
Adherence to urticaria management guidelines by resident doctors in dermatology department in our institute was satisfactory.
Early-Onset Versus Late-Onset Psoriasis
Authors: Fatema Farhat , Ghoshal Loknath , Saha Abanti , Agarwal Sristi , Bandyopadhyay Debabrata 
Published: April 2022

Background:
Psoriasis is a chronic inflammatory diseaseresulting from a complex interplay of genetic and environmental factors affecting the skin, nail and joints. Two distinct types of psoriasis are said to exist (i) early onset psoriasis (EOP), beginning before the age of 40 years and (ii) late onset psoriasis (LOP), beginning ≥40 years; with the presence of Human lymphocyte antigen (HLA) Cw6, present in majority of patients with early onset. Several studies demonstrated clinical and genetic differences between EOP and LOPamong European and East Asian populations. Lack of similar study in the Indian population has prompted us to undertake the present work.

Aims and Objectives:
(i) To compare the clinical patterns of early onset and late onset psoriasisin patients attending the Dermatology outpatientdepartment (OPD) and admitted in the in-patient department (IPD). (ii) To analyze the association age of onset with presence of HLA Cw6.

Materials and Methods:
It was an institution-based, descriptive, cross-sectional study. Consecutive patients with psoriasis at the OPD and IPD of the department of Dermatology during the study period, were recruited in the study after obtaining informed consents. Detailed history was obtained regarding the disease, co-morbidities and complications. Through physical examination was carried out, PASI was calculated and blood samples were drawn fromconsenting adult patients (age>/=18 years) to study the presence of Cw6.

Results:
The study population (n=250) wasbroadly divided into “Early onset psoriasis(EOP)” (n=138) and Late onset psoriasis (LOP)” (n=112).Significant higher occurrence of positive family history, nail involvement and koebnerization were found in EOP, but such differences were absent considering the types, patterns, joint involvement, severity and HLACW6 positivity.

Conclusion:
This study supports the concept of two subtypes of psoriasis based on age of onset showing different clinical and evolutionary features.
Contact Allergy to Ammonium Persulfate
Authors: Alajaji Abdullah , Scheinman Pamela 
Published: April 2022

Background:
Persulfate is an oxidizing agent used for multiple purposes, including bleaching hair, bleaching flour, and as pool/spa water disinfectant.

Objective:
To identify the prevalence and clinical characteristics of patients allergic to ammonium persulfate (APS) among patients who underwent patch testing in our clinic.

Methods:
A retrospective chart review of 2138 patients who underwent patch testing at Brigham and Women's hospital in Boston between July 2015 to November 2019. All patients were tested to our standard series including APS 2.5% petrolatum. Given the irritant nature of APS, we included for analysis only patients with 2+ or 3+ reactions.

Results:
Among 2138 patients, 61 (2.85%) had 2+ or 3+ reactions to APS. In this study, 72% were female. 75% had an atopic diathesis; 56% had 2+ reactions, and 44% had 3+ reactions. Clinical relevance was definite in 11%, probable in 10%, and unknown/possible in 79%. In patients with definite clinical relevance, 6/7 had occupational allergic contact dermatitis: 4 hairdressers, 1 hair salon cleaner, and 1 aquarium worker; 1/7 was bleaching her hair.

Conclusion:
Prevalence of 2+ or 3+ APS reactions in this single-center study was higher than that reported by the North American Contact Dermatitis Group, 2015–2016, (0.75% vs. 2.85% in our study). APS is an important occupational allergen, and clinicians should be aware of this when treating patients with exposure to APS.