bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Mechanisms of Notopterygii rhizoma Et Radix for Treating Arrhythmia
Based on Network Pharmacology
Authors: Wei Penglu , Shang Juju , Liu Hongxu , Xing Wenlong , Tan Yupei 
Published: November 2022

Objective:
To explore the possible mechanism for treating NRR in arrhythmia using
network pharmacology and molecular docking in this study.

Methods:
Active compounds and targets for NRR were retrieved from the Traditional Chinese
Medicine Systems Pharmacology (TCMSP) Database and Analysis Platform, SymMap, and the
Encyclopedia of Traditional Chinese Medicine (ETCM) databases. Arrhythmia-related genes were
acquired from the Comparative Toxicogenomics Database (CTD) and the GeneCards database.
Overlapping targets of NRR associated with arrhythmia were acquired and displayed via a Venn
diagram. DAVID was applied for GO and KEGG pathway analyses. Cytoscape software and its
plug-in were used for PPI network construction, module division and hub nodes screening. Auto-
Dock Vina and qRT-PCR were carried out for validation.

Results:
In total, 21 active compounds and 57 targets were obtained. Of these, coumarin was the predominant
category which contained 15 components and 31 targets. There were 5 key targets for NRR
in treating arrhythmia. These targets are involved in the apoptotic process, extrinsic apoptotic signaling
pathway in the absence of ligand, and endopeptidase activity involved in the apoptotic process by
cytochrome c. The main pathways were the p53 signaling pathway, Hepatitis B and apoptosis. The
molecular docking and qRT-PCR displayed good effects on hub node regulation in NRR treatment.

Conclusion:
NRR plays an important role in anti-apoptotic mechanisms that modulate the p53 signaling
pathway, which may provide insight for future research and clinical applications focusing
on arrhythmia therapy.
The Determination of Molecular and Toxicological Mechanisms of Cucurbitacin
E in Model Organism Drosophila melanogaster and Various Cancer
Cell Lines: Molecular Modelling, Docking and Dynamic Simulation
Studies
Authors: Tunçbilek Aydın , Azarkan Serap Yalçin , Ercan Fahriye 
Published: November 2022

Introduction:
Cucurbitacins are one of the most important components of Ecballium elaterium. Among the cucurbitacins, Cucurbitacin E was the first to be isolated. This study focused on screening the anticancer and insecticidal potential of Cucurbitacin E by the in-vitro, in-vivo, and in-silico methods.

Methods:
In the study, toxicity analysis of Cucurbitacin E were determined on HeLa, Caco 2 cancer cell lines and D. melanogaster. While the expression levels of the BAD, BCL-2, AKT-1 and H-purine genes of cancer cell lines were determined, the CG15530, BUFFY, AKT-1 and Purine genes of D. melanogaster were determined by RT-PCR. Besides, molecular docking and dynamic properties of Cucurbitacin E with human and insectoid enzymes were presented in silico.

Results:
The IC50 value of Cucurbitacin E in the HeLa ovarian and Caco 2 colon cancer cell lines was determined to be 42 ug/ml and 85 ug/ml, respectively. The LC50 and LC99 doses for fruit flies were determined to be 47,693 µg/ml and 133,251 µg/ml, respectively. Gene expression analysis revealed that Cucurbitacin E showed the greatest effect on Purine and AKT-1 genes in D. melanogaster. We analyzed all genes by Western blot, but we did not detect significant changes in genes other than H-purine.
In silico studies revealed that the Purine protein of D. melanogaster had the highest bonding energy with Cucurbitacin E as a ligand. Similarly, Cucurbitacin E showed great affinity towards H-purine (-10.2 kcal/mol). Molecular dynamics simulation studies were also performed to determine the stability of the dynamic process.

Conclusion:
Conclusion: As a result of our in vivo, in vitro and bioinformatic analyzes, it has been seen that Cucurbitacine E is effective against the cancer types and model insect studied.
Recent Reviews on Dynamic Target Detection Based on Vision
Authors: Zhang Hongxin , Song Ruijin , Jiang Hanghang 
Published: November 2022

Background:
Vision-based dynamic target detection is an important research topic in
computer vision, which is the basis for intelligent behavior analysis and event detection. Further
research on dynamic target detection methods can help improve target detection and tracking mechanisms
while also driving the development of other related fields. Hence, conducting a review on
vision-based dynamic target detection is very significant.

Objective:
There are many methods for dynamic target detection. This paper introduces their classification,
characteristics, advantages, disadvantages and development trends.

Method:
This paper reviews recent patents and representative articles on dynamic target detection
in simple visual and complex contexts. The crucial methods of these references are introduced from
the aspects of algorithm, innovation, and principle.

Results:
This paper analyzes and compares the existing dynamic target detection methods, summarizes
their characteristics, main applications, and advantages and disadvantages in the current development
stage, and discusses the future development and potential problems of dynamic target tracking
methods.

Conclusion:
Vision-based dynamic target detection can accurately extract moving targets from the
scene. Due to its inherent complexity, each detection method has its advantages and disadvantages
in specific scenes. Currently, the research mainly focuses on the real-time robustness and accuracy
of the algorithm, which needs to be further improved in the aspects of algorithm innovation, multialgorithm
fusion, multi-target recognition, and algorithm adaptability. Therefore, relevant research
patents and documents should be put forward, initiating the future development trend.
Assessment of Parent and Nurse Perceptions of Parental Participation in Hospital Paediatric Care
Authors: Shdaifat Emad , Sudqi Abdallah M. , Abusneineh Firas T. , Al-Ansari Najla M. 
Published: November 2022

Background:
Parent and nurse perceptions about the care of hospitalized children have been examined in developed countries, but little is known in developing countries.

Objective:
The study aims to evaluate the perceptions of nurses and parents towards parental participation in care for hospitalized children and associated factors.

Methods:
A cross-sectional design was used to collect data from nurses and parents in paediatric units in the Eastern Region of Saudi Arabia using a convenience sampling technique. Data were collected using the Parent Participation Attitude Scale (PPAS), and the agreement and differences between groups were analysed.

Results:
The present results found that both parents and nurses agreed on 7 out of 20 statements regarding parental involvement in care for their children. Nurses in paediatric surgery had a higher perception of involving parents (P = 0.002), while nurses in Well Baby Nursery (WBN) ward had the lowest perception. The present findings confirm that married caregivers had higher perceptions (P = 0.035) than their single counterparts.

Conclusion:
Parent participation attitude was agreed on for seven statements between parents and nurses. More studies are required to explore the relationship between healthcare providers and parents with regard to parental participation in caring for hospitalized children.
Cedera olahraga pemain baseball dan softball di Daerah Istimewa Yogyakarta
Authors: Suryobroto Agus Sumhendartin , Budayati Eka Swasta , Anugrah Tama 
Published: November 2022

Tujuan penelitian ini untuk mengidentifikasi cedera pada pemain baseball dan softball di Daerah Istimewa Yogyakarta (DIY). Identifikasi meliputi, jenis cedera, bagian tubuh yang cedera dan penyebab terjadi cedera. Jenis penelitian ini deskriptif dengan metode survei. Subjek yang digunakan pemain baseball dan softball di DIY. Sampel sebanyak 33 orang, 18 pemain baseball dan 15 pemain softball dengan menggunakan teknik pengambilan sampel purposive sampling. Instrumen yang digunakan adalah angket. Teknik analisis data dalam penelitian menggunakan perhitungan statistik deskriptif persentase, yaitu dengan cara mengadakan persentase dan penyebaran serta memberikan penafsiran yang diperoleh atas dasar persentase tersebut. Hasil penelitian secara keseluruhan terkait cedera pada olahraga baseball dan softball dapat diidentifikasi dari jenis cedera nyeri (sprain/strain) sebesar 29,59 %, cedera memar 24,13 %, lecet 16,36 %, kram dan perdarahan 10,58 %, dislokasi 7,44 %, dan fraktur 1,32 %. Bagian tubuh yang cedera dapat diidentifikasi pada bagian tungkai dan kaki 34,87%, lengan dan tangan 33,60%, badan 24,09%, dan kepala 7,45%. Sedangkan dari segi penyebab cedera dapat diidentifikasi bahwa terkena bola 30,59%, overuse dan terlalu lelah 29,64%, sliding dan jatuh 19,49%, benturan dengan pemain lain 9,67%, lain-lain (sepatu yang sempit, geseran grip bola, dan pegangan bat) 6,18%, kurang pemanasan 3,49%, dan teknik yang salah 0,95%. Sports injury of baseball and softball players in the Special Region of YogyakartaAbstractThe purpose of this study was to identify injuries to baseball and softball players in Special Region of Yogyakarta (DIY). Identification includes, the type of injury, the injured body part and the cause of the injury. This type of research is descriptive with a survey method. Subjects used by baseball and softball players in DIY. The sample is 33 people, 18 baseball players and 15 softball players using purposive sampling technique. The instrument used is a questionnaire. The data analysis technique in this study uses descriptive statistical calculations of percentages, namely by holding percentages and distributions and providing interpretations obtained on the basis of these percentages. The overall results of baseball and softball injuries can be identified by the type of pain injury (strain/strain) 29.59%, bruises 24.13 %, abrasions 16.36%, cramps and bleeding 10.58 %, dislocations 7.44 % , and fracture 1.32%. Injured body parts can be identified as legs and feet 34.87%, arms and hands 33.60%, body 24.09%, and head 7.45%. The causes of injury can be identified that hit by the ball 30.59%, overuse and too tired 29.64%, sliding and falling 19.49%, collision with other players 9.67%, others (narrow shoes, sliding ball grip and bat grip) 6.18%, lack of heating 3.49%, and wrong technique 0.95%.
Unmasking the Twitter Discourses on Masks During the COVID-19 Pandemic: User Cluster–Based BERT Topic Modeling Approach
Authors: Xu Weiai Wayne , Tshimula Jean Marie , Dubé Ève , Graham Janice E , Greyson Devon , MacDonald Noni E , Meyer Samantha B 
Published: November 2022

Background
The COVID-19 pandemic has spotlighted the politicization of public health issues. A public health monitoring tool must be equipped to reveal a public health measure’s political context and guide better interventions. In its current form, infoveillance tends to neglect identity and interest-based users, hence being limited in exposing how public health discourse varies by different political groups. Adopting an algorithmic tool to classify users and their short social media texts might remedy that limitation.

Objective
We aimed to implement a new computational framework to investigate discourses and temporal changes in topics unique to different user clusters. The framework was developed to contextualize how web-based public health discourse varies by identity and interest-based user clusters. We used masks and mask wearing during the early stage of the COVID-19 pandemic in the English-speaking world as a case study to illustrate the application of the framework.

Methods
We first clustered Twitter users based on their identities and interests as expressed through Twitter bio pages. Exploratory text network analysis reveals salient political, social, and professional identities of various user clusters. It then uses BERT Topic modeling to identify topics by the user clusters. It reveals how web-based discourse has shifted over time and varied by 4 user clusters: conservative, progressive, general public, and public health professionals.

Results
This study demonstrated the importance of a priori user classification and longitudinal topical trends in understanding the political context of web-based public health discourse. The framework reveals that the political groups and the general public focused on the science of mask wearing and the partisan politics of mask policies. A populist discourse that pits citizens against elites and institutions was identified in some tweets. Politicians (such as Donald Trump) and geopolitical tensions with China were found to drive the discourse. It also shows limited participation of public health professionals compared with other users.

Conclusions
We conclude by discussing the importance of a priori user classification in analyzing web-based discourse and illustrating the fit of BERT Topic modeling in identifying contextualized topics in short social media texts.
Cytotoxic Tobacco Smoke in The Cornea of Wistar Rats Fed Turmeric (Curcuma Domestica Val.) Extract
Authors: Kusumawardani Agapietta , Sarwendah Kurniati , Rahmad Laelatul , Milah Nida Ul , Herliyani Novi , Sutrisno Bambang , Wuryastuty Hastari , Wasito R 
Published: November 2022

Abstract An increasing number of smokers who tend to rise rapidly from year to year has become a major focus of public health issues. Exposure to environmental tobacco smoke, containing toxic chemicals can trigger acute or chronic inflammation and oxidative stress in the eyes. To overcome this condition, phytotherapeutic and/or phytopreventive approaches which are efficatious, safe and cheap is needed. One of spices from Indonesia which is greatly possible to be promoted as a herbal medicine, is a phytochemical curcumin in turmeric. This present study was conducted to identify and determine the effects of turmeric extract (Curcuma domestica Val.) as phytotherapeutic and/or phytopreventive in the eyes lesion of Wistar rats induced by cigarette smokes. Twenty male Wistar rats at 3 months old were divided randomly into four groups of five each. Those were the control group (KI), the group given 9 cigarettes smoke/day (K II), the group given turmeric extract 1 ml 0, 25% /day (K III) and the group given 9 cigarettes smoke/day and 0, 25% turmeric extract 1 ml /day (K IV) for 60 days. Water and feed was given ad libitum. At the end of the experiment, all rats were euthanized with ether, necropsied andeyes balls were collected, fixed formalin 10% and stained with routine hematoxylin-eosin. The results of the present study indicated that 9 cigarettes smoke/day increased mitotic activity and caused hyperplasia in the corneal epithelial cells (K II), and addition of turmeric extract 0, 25% 1 ml per oral for 60 days prevented both mitotic activity and hyperplasia in the corneal epithelial cells (K IV). Extract of turmeric (Curcuma domestica Val.) has been proved to have herbal therapeutic and/or preventive effects againts the pathotoxicological cigarette smokes. Further study needs to be done to more evaluate pathogenesis of the eyes lesions and phytotherapeutic and/or phytopreventive effects of turmeric extract as well.
Hypertropic Osteopathy (Marie Diseases) in The German Shepherd Dog
Authors: Adji Dhirgo 
Published: November 2022

Abstract A Male German Shepherd dog, five years old, twenty six kg of body weight was submitted to Professor Soeparwi Animal Hospital, Faculty of Veterinary Medicine, Gadjah Mada University by Mr. Siswo Han, the owner who lives in Cangkringan, Yogyakarta on May 13, 2013. The dog was suffered from the pain and lameness on the front-left extremity for about 2 weeks. The dog having registered number of 8738/V/A/RSH/13 did not show any specific clinical signs, except the lameness. Skin and hairs, mucous membranes, lymphoid glands, and respiratory, digestive, circulatory and uropoetica organs as well were within the normal limits. The result of standard blood test for both red blood cell and white blood cell were still within normal values. Evaluation of fecal sample of the dog was also normal meaning that no parasites were found in the fecal sample. Radiographic analysis of the upper left extremity and the lungs area showed that there was an irregular bone growth on the left caput humeri, and the lungs were normal. Based on the lesion, the dog was diagnosed as having a hyperthropic osteopathy (Marie Disease).
Cytotoxicity Study of Red Fruit Extract (Pandanus conoideus Lam) as Anti-Goitrogenic Herbal Medicine in Sprague Dawley Rats
Authors: Wuryastuty Hastari , Wasito R. 
Published: November 2022

The use of herbal medicine (phytopharmaca) has increased manifold because of the safety. Red fruit extract is one of the supplements that has many advantages for health. The efficacy of pre-clinical trial using the rats induced by iodine deficient ration has proven that 1-2 ml of red fruit extract supplementation with/without KIO3 fortification for 30, 60 and 90 days has potency as anti-goitrogenic herbal medicine. For standarization, however, the extract red fruit has to be tested for the safety in the important organs like livers and kidneys. The research results have proven that in the normal Sprague Dawley rats, supplementation of 2 ml/day/rat of red fruit extract for 135 consecutive days has.no toxic effect in the livers and kidneys. In hyperplastic goiter Sprague Dawley rats induced by goitrogenic ration, the red fruit extract supplementation has to be in a combination with KIO3 to overcome iodine deficiency disorders and has no-toxic effect to the livers. Therefore, it can be concluded that based on pre-clinical studies using Sprague Dawley rats, the red fruit extract can be categorized as standardized anti-goitrogenic herbal medicine.
Total Plate Count of Milk from Dairy Cooperatives in Yogyakarta and East Java
Authors: Septiani Monica , Drastini Yatri 
Published: November 2022

Most of the local populations in Indonesia, especially in Sleman, Yogyakarta and Tulungagung, East Java consume cow milk. Cow milk is more widely consumed than that of horse or goat because of the people habit and the raw milk is more easily found in the market. The majority of people consume milk without knowing the number of bacteria contained and neither the presence nor absence of harmful bacteria in the milk consumed. This study aimed to identify the total number of bacteria in several dairy cooperatives in Sleman, Yogyakarta and Tulungagung, East Java. Milk samples were taken from two tanks in Koperasi Sarono Makmur (KSM), three tanks (were taken four times, once a week) in Koperasi Warga Mulya (KWM) Sleman, Yogyakarta, and two tanks (twice in the morning and once in the evening) in Koperasi Unit Tani Ternak (KUTT) Tulungagung, East Java. Milk from each tank were taken 500 mL and put into the cooler that was given ice cubes. Then the samples to be tested for total bacteria from each tank with plate count agar medium. The number of samples was 19. Milk samples were statistically analyzed with one way ANOVA. The results showed that the total number of bacteria of milk of KSM is 1.0 x 106 CFU/mL, KWM 3.6 x 106 CFU/mL, whereas cow's milk KUTT 4.0 x 106 CFU/mL. The average total number of bacteria of KWM and KUTT exceeds the requirements of the Indonesian National Standard (SNI) 01-3141-1998 (1,0 x 106 CFU/mL).
A Study of the Fruit Bat (Rousettus sp) Brain Anatomy as Natural Reservoir Wild Animal for the Rabies Virus
Authors: Sari Karina Mayang , Khomariyah Siti , Arofah Nur , Wardhani Sabrina Wahyu , Pangestiningsih Tri Wahyu 
Published: November 2022

Rousettus sp. (Fruit bat) is one type of fruit bats in Indonesia and act as a natural reservoir of rabies. Rabies is caused by a virus from genus Lyssavirus, family Rhabdoviridae, which attack central nervous system (CNS).The brain is an organ that is sensitive to rabies infection. The purpose of this study was to determine the anatomical structure of the fruit bat brain macroscopically. Five fruit bat were used in this study, they were anaesthetized using ketamine and xylazin. Animals were perfused using physiological saline and 10% buffered formalin. Brains were taken using tweezers after all the bones of the skull were separated. Analysis of macroscopic brain was done descriptively. The results showed that the fruit bat brain were generally divided into cerebrum, cerebellum and brain stem. Gyrus, sulcus and the paraflokulus lobes of the fruit bat brain were less developed than that of the dogs brain.
Gastrointestinal Endoparasites Diversity of Macaca fascicularis in Goa Monyet Tenau Garden, Kupang
Authors: Oka Wirawan I Gusti Komang , Kusumaningrum Damai , Oematan Aven Bernard 
Published: November 2022

Goa Monyet Tenau Garden is one of the tourist parks located in Kupang East Nusa Tenggara Province. The population of long-tailed macaque (Macaca fascicularis) is about 142 in the region, while outside the region is 162. This study was aimed to determine the types of gastrointestinal endoparasites that infect monkeys which live inside and outside the region, and to study if there is a relationship between different regions with percentageof gastrointestinal endoparasites that infect monkeys as well. Specific target was to obtain data on the diversity of endoparasites in the tourist park. Macaca fascicularis fecal sampling was done randomly from inside and outside the region. Thirty fecal samples were taken which composed of 15 samples from the region and 15 samples from outside the region. The weight of each fecal sample is ± 10 gr. Each fecal sample was inserted into the pot(capacity ± 25 ml) and labeled by the place, date and month of collection. The samples were given 10% formalin as a preservative. Examinations were conducted at the Animal Health Laboratory Politani Kupang, using 2 methods : Centrifuge and Sedimentation method. Diversity and percentage of worm infection in monkeys between different regions were analyzed descriptively. The results showed that the types of endoparasitesinfecting monkeys inside and outside of the region were the same (Eimeria sp., Ancylostoma sp., and Strongyloides sp.). This was caused by the interaction of the use of the same region. Percentage average of endoparasitic infection that infects monkeys in the region was higher than that of outside the region (In the region: Eimeria sp./ 86.7%, Ancylostoma sp./ 73.3% and Strongyloides sp./66.7%, outside the region: Eimeriasp./ 80%, Ancylostoma sp./60%, Strongyloides sp./46.7%. The difference percentages of infection were influenced by several factors, such as vegetation, water sources, sanitation, season, temperature and population.
Cloning of the gene encoding Endo-β-1,3-1,4 Glucanase from Bacillus subtilis subsp. spizizenii W23 on Plasmid pMMB67EH into Escherichia coli DH5α and Escherichia coli Origami
Authors: Gunawan Lisa 
Published: November 2022

One of the genes encoding cellulolytic enzymes, named BSUW23_10175, from Bacillus subtilis subsp. spizizenii W23 was cloned in the plasmid pMMB67EH into Escherichia coli DH5α and Escherichia coli Origami. Target gene was amplified using polymerase chain reaction (PCR) with specific primers (For- BSUW23_10175 dan Rev-BSUW23_10175) that were used for BSUW23_10175 gene amplification. Based on the PCR and restriction analyses of the transformants plasmid, it was known that clones number 8 and 9 of the Escherichia coli Origami transformants contained the recombinant plasmid pMMB_BSUW23_10175.Cellulolytic activity assay showed that those transformants had cellulolytic activity by indution with IPTG (isopropyl-β-D-thio-galactosidase).