bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of vaginal microecology and immunity on the pregnancy outcome of cervical cerclage
Authors: Cai Shengnan , Wu yanting , Zeng li , Ding yiqian 
Published: April 2022

Abstract
Objective To investigate the effects of vaginal microecology and immune status on the pregnancy outcome of cervical cerclage. Methods The clinical data of 125 patients with cervical incompetence who underwent transvaginal cervical cerclage in our hospital from January 2018 to January 2021 were collected, based on which the associations of vaginal microecology and related immune cytokines (IL-1β, IL-6, IL-8 and TNF-α) with the cervical cerclage outcome were explored. Results All of the 125 patients had singleton pregnancies, who were aged 20–43 years, with a mean of (32.34±5.17) years. The surgery was successful in 104 patients, while unsuccessful in 21 patients, revealing a success rate of 83.20%. There were 70 full-term deliveries, 34 premature deliveries (28 survived while 6 died), and 15 late miscarriages. Univariate analysis revealed statistically significant differences in the timing of surgery, gestational age of cerclage, preoperative cervical canal length, genital tract infections and preoperative invasive procedures between the successful and unsuccessful groups (P<0.05). Insignificant differences were found in the pre-pregnancy body mass index (BMI) (P>0.05). According to the multivariate analysis results, cerclage timing and genital tract infections were independent risk factors for postoperative pregnancy failure (P<0.05). The pathogen detection rates in the two groups of pregnant women were analyzed, finding significantly higher incidence of bacterial vaginosis (BV) in the unsuccessful group than in the successful group (P<0.05). Inter-group comparison revealed that the positive rates for vaginal microenvironmental factors (LE, NAG, SNA, H2O2 and pH) were all significantly higher in the unsuccessful group than in the successful group (P<0.05). Besides, the immune cytokine levels in the cervicovaginal secretions were also all significantly higher in the unsuccessful group than in the successful group (P<0.05). Conclusion The pregnancy outcome of patients undergoing cervical cerclage is associated with the imbalance of vaginal microecology and the levels of IL-1β, IL-6, IL-8 and TNF-α in cervicovaginal secretions.
NHS staff awareness, attitudes and actions towards the recent change in organ donation law in England-results of the #options survey.
Authors: Coe Dorothy , Newell Natasha , Jones Mark , Robb Matthew , Clark Natalie , Reaich David , Wroe Caroline 
Published: April 2022

Abstract
BackgroundIn Spring 2020 there was a change in organ donation legislation in England (UK). Whilst much is known about public opinions to organ donation and the change in legislation, there is little evidence about the opinions of the NHS workforce. This study was set up to understand the levels of awareness, support and action of NHS staff to this change and to explore the impact of respondent demographics, place and type of work on support, awareness and action.MethodsAn online survey was offered to all NHS organisations in North Thames and the North East and North Cumbria through the NIHR Clinical Research Network between July and December 2020. Participating organisations were provided with an information package and promoted the survey via email and internal staff communications. Analysis was completed using Statistical Analysis Systems software with differences in categorical variables compared using Chi-squared tests.ResultsA total of 5789 staff participated in the survey. NHS staff respondents were supportive of the change in organ donation legislation; this support was most prominent in the Ambulance Service, but found in all sections of the NHS. Across almost all demographic variables, NHS staff were more aware of the change in legislation than the General Public. NHS staff respondents were much more likely to already be on the organ donation register, more likely to have had a conversation with partner/family and less likely to have registered a decision not to donate than the General Public.ConclusionsNHS staff were more aware, supportive and more likely to have taken positive action around the change in organ donation legislation, even across religious and minority ethnic groups. The size and ethnic diversity of the NHS workforce offers huge opportunity to enable and support NHS staff to be community advocates for organ donation and to raise awareness of the change in legislation amongst their communities.Study registrationThe study involved NHS staff and did not require full ethical approval. It was reviewed and approved by a Health Research Authority assessor. IRAS number: 275992
In vitro compatibility of Brassicaceae extracts with nematophagous fungi and their effects against Nacobbus celatus
Authors: Sosa Ana Laura , Girardi Natalia Soledad , Rosso Laura Cristina , Etcheverry Miriam Graciela , Passone María Alejandra 
Published: April 2022

Abstract
Nacobbus celatus sp. n. is one of the main root-knot nematodes in the field destined for horticultural production of the central region of Argentine due to its ability to infect several host plants. The lack of new and safe active ingredients against this nematode has restricted control alternatives for growers. Egg-parasitic fungi and biofumigation with brassicaceae have been considered as potential candidates for the development of bionematicides. Nematicidal effects of Brassica oleracea var. italic (broccoli) and Brassica oleracea var. capitata (cabbage) aqueous extracts (AEs) against second-stage juveniles (J2) of N. celatus were evaluated in vitro. Fisher LSD tests evidenced significant nematicidal (α = 0.05) effects of the two AEs tested, with LD100 of 250 and 500 µL mL − 1 for broccoli and cabbage, respectively. Compatibility assays between AEs and five nematophagous fungi were performed on soil extract solid medium (SESM) conditioned at 0.99 water activity and incubated at 30, 25 and 20°C. Purpureocillium lilacinum SR14 was the fungal strain that showed compatibility at levels of spore viability, growth rate and conidia productions at LD50 (125 µL mL− 1) and LD25 (60 µL mL− 1) of broccoli aqueous extract (BAE) y enhanced the nematophagous effect. Moreover, phytotoxic studies revealed that the 125 of BAE applied at the transplantation time could be safely used without affecting tomato culture. In conclusion, the integrated application of BAE with the nematophagous fungus, which combines two action mechanisms, represent a promising integrated strategy to control phytoparasitic nematodes.
A novel enantioseparation and trace determination of chiral herbicide flurtamone using UPLC-MS/MS in various food matrices and environmental based on Box-Behnken Design
Authors: Zhang Yanqing , Li Rui , Tan Yuting , Chen Zihao , Sang Ningning , Wang Zhen , wang Minghua , Shi Haiyan 
Published: April 2022

Abstract
The detection method for flurtamone enantiomers in soil and five food crops (wheat, maize, Sorghum, soybeans and pea) was established using modified QuEChERS method combined with ultra-high-performance liquid chromatography tandem triple quadrupole mass spectrometry (UHPLC-MS/MS). In this study, the Box-Behnken Design (BBD) of response surface methodology was used to optimize the enantioseparation parameters of flurtamone on Chiralpak IG-3 column. The absolute configuration was confirmed by the specific rotation and electron circular dichrometry(ECD). The elution order was confirmed as R-(-)-flurtamone and S-(+)-flurtamone with resolution (Rs) 4.10 on Chiralpak IG-3 column. Under optimal conditions, the average recoveries in six matrices ranged from 78.2% to 115.7%. The intraday relative standard deviations (RSDs) were less than 11.9%, and the interday RSDs were less than 7.9% for the two enantiomers. The limits of detection was 0.005 mg kg−1 for flurtamone enantiomers in six matrixs. Finally, the method was applied to the enantioselective degradation of flurtamone enantiomers in soil. R-(-)-flurtamone was degraded faster than S-(+)-flurtamone in soil, causing the EF of the enantiomers to change from 0.50 to 0.42 in 28 d. The method for residue analysis of flurtamone in food and environmental samples was established for the first time and applied to actual samples. The proposed method provides reliable technical support for food sample analysis and environmental risk assessment of flurtamone.
EEG-Based Drowsiness Analysis to Establish Driving Safety Using Deep Learning Approaches
Authors: s priyanka , s shanthi 
Published: April 2022

Abstract
Drowsiness is the inactivated state of the brain and observed during the transition from awaken state to sleepy state. This inactive state diminishes an individual's attention and leads to accidents during professional or personal activities. The prediction of this inactive (drowsiness) state using AI plays a substantial role in the avoidance of accidents. The advancements in the field of Artificial Intelligence and Neuroscience approaches are used for the prediction of this inactive drowsy state. In order to prevent these devastating accidents, the state of drowsiness of the driver has to be be monitored. Electroencephalogram (EEG) is a predominant tool adopted to examine various states of the brain effectually. It is generally known as Brain-Computer Interface System. The EEG channels are used for predicting the inactive state while implementing the real-time applications. However, the researchers face various challenges during execution based on the classification and channel selection process. This research concentrates on modelling and efficient drowsiness prediction methods and intends to bridge the gap encountered in the existing approaches. A novel stacked Long-Term Short Memory \((s-LSTM)\) with Deep Fully Connected- Convolutional Neural Network (\(DFC-CNN\)) is used to learn and memorize the long-term feature dependencies and attains essential information based on time-series prediction. Single and multi-channel EEG data is considered to measure the statistical characteristics of available EEG signals. The online available OpenBCI sleep analysis data is used for performing the experimentation, and the simulation is done in a Pycharm and run in GoogleColab environment. The proposed \(s-LSTM\) model provides a better trade-off compared to existing approaches. The model generalization is improved with the validation of combined feature subjects. Here, metrics like prediction accuracy, RMSE, false positives, scaling coefficients related to false positives are measured to show the significance of the model.
WITHDRAWN: Influence of Genetic Polymorphisms on the Pharmacokinetics and Safety of Nifedipine Controlled-release Tablets in Healthy Volunteers
Authors: Li Zhen , Wang Siyang , Ren Jian , Zhi Tingting , Wang Hongxia , Zhu Yuanyuan , Yao Zhiqing , wang Yanhui , Wang Huizi , Zhang Ruiqing , Wang Xiaoru 
Published: April 2022

Abstract
Purpose: Nifedipine is a calcium channel blocker, which is used to treat hypertension and angina pectoris. The present study aimed to evaluate the effects of gene polymorphisms on pharmacokinetics of relevant metabolic genes with inconsistent or incomplete research conclusions and of others that exhibited an impact on pharmacodynamics, so as to provide a scientific basis for the inter-individual variation in nifedipine controlled-release tablet disposition.Methods: A total of 33 healthy volunteers were administered a single 30 mg oral dose of nifedipine controlled-release tablets, and were genotyped for 17 SNPs within 12 genes, by StepOne Real-Time PCR System and the Matrix-assisted laser desorption ionization-time of flight. The plasma concentration levels were quantified by Ultra-performance liquid chromatography tandem mass spectrometry. Univariate and multivariate analyses were performed and the mean pharmacokinetic variables were compared according to genotypes. Results: The CYP3A4 rs2242480, ABCB1 rs1045642, CACNA1D rs312481, CACNA1C rs2238032, ACE rs4646994 and SLC14A2 rs3745009 polymorphisms significantly influenced the pharmacokinetics of nifedipine controlled-release tablets with a greater effect being noted on CACNA1D, CACNA1C, and SLC14A2. These results continued to be statistically significant following multivariate correction. Conclusions: The CYP3A4 rs2242480, ABCB1 rs1045642, CACNA1D rs312481, CACNA1C rs2238032, ACE rs4646994 and SLC14A2 rs3745009 polymorphisms significantly influenced the pharmacokinetics of nifedipine controlled-release tablets . Their pharmacokinetic properties were examined, so as to provide a theoretical basis for individual differences found during treatment.
Effect of quantified table ware use on ABC goals in patients with type 2 diabetes: portions, proportions and perceptions
Authors: Leung Ching-Hsiang , Tzeng Min-Su , Tsai Chia-Ying , Tsai Wan-Rong , Liu Sung-Chen , Hsu Pi-Hui , Chuang Shih-Ming 
Published: April 2022

Abstract
Aims: This study examined the effect of 12 months use of quantified tableware (QTW) on glycemic control in patients with type 2 diabetes (T2DM).Methods: In this prospective, randomized, observational study, 94 adult T2DM patients from the Diabetes Shared Care Network with HbA1c >7% were randomly assigned to control (n=47) and intervention (n=47) groups. All were followed up at the out-patient clinic. The intervention group were given a set of QTW to use. HbA1c, BP and serum LDL cholesterol (LDL), the (ABC) goals were defined as HbA1c <7%, BP <140 mmHg systolic and <90 mmHg diastolic, and LDL <100 mg/dL.Results: Seventy-seven patients, 43 in the control group and 34 in the intervention group completed the study. After 12 months, fasting glucose (AC) reduced significantly in the control group, while the intervention group had significantly decreased AC, 2-hour postprandial glucose (PC), HbA1c and LDL. Achievement of LDL and 2 ABC goals significantly increased only in the intervention group. All ABC goals were achieved by 21.9% in the intervention group and 12.8% in the control group.Conclusions: Use of quantified tableware, a low-cost nutritional intervention for 12 months improved AC, PC, HbA1c and LDL cholesterol levels in T2DM patients.
A retrospective, observational cohort study of pharmacist telehealth services in an underserved population with type 2 diabetes mellitus
Authors: Mukherjee Sumana Mimi , DelDotto Dana , Patel Aesha , Silva Matthew A. 
Published: April 2022

Abstract
Background: To safely provide comprehensive medication management during the COVID-19 pandemic, clinical pharmacists caring for outpatients with type 2 diabetes mellitus at a Federally Qualified Healthcare Center shifted to providing telehealth, primarily via telephone. Aim: This study evaluated the effectiveness of clinical pharmacist telehealth for these underserved patients, and identified associations between characteristics and efficacy measures. Method: Patients with uncontrolled type 2 diabetes (hemoglobin A1c greater than or equal to 8%) with a clinical pharmacist visit between April 1 and August 31, 2020 were included in the retrospective cohort study. The effectiveness of telehealth was measured by the percentage of patients reached, the percentage of appointments completed, and the change in hemoglobin A1c from baseline. Interventions by the clinical pharmacist were analyzed as a secondary outcome. Results: There were 181 patients scheduled and 172 (95%) of those patients interacted with a clinical pharmacist at least once. Of the 667 appointments scheduled, 72.9% were kept. Median hemoglobin A1cs changed from 10.2% to 9.2% and 24.6% achieved a hemoglobin A1c less than or equal to 8% (n = 138, p-value < 0.0001 for both). The healthcare quality impact of interventions by appointment was favorable; 83.3% were significant (brought care to a higher standard), 1.9% were very significant (averted major organ dysfunction) and 0.4% were extremely significant (prevented death). Conclusion: Clinical pharmacist telehealth was effective for providing care and lowering hemoglobin A1cs for many patients during the pandemic when office visits were not an option.
MRCKα mediates Met- and Leu-stimulated β-casein synthesis in bovine mammary epithelial cells via targeting mTOR
Authors: Fang Fang , Baal Jürgen van , Ma Lu , Gao Xuejun , Dijkstra Jan , Bu Dengpan 
Published: April 2022

Abstract
Background
Amino acids (AA), including methionine (Met) and leucine (Leu), stimulate milk synthesis in bovine mammary epithelial cells (BMEC) via activation of protein kinase mechanistic target of rapamycin (mTOR). Here, we further explored the possible role of myotonic dystrophy-related CDC42-binding kinase alpha (MRCKα), previously shown to be a critical mediator of prolactin-stimulated milk synthesis in BMEC.
Results
Application of various doses (0, 0.2, 0.4, 0.6, 0.8 mM) of Met or Leu to a primary culture of BMEC revealed an optimal dose on stimulation of β-casein production of 0.6 mM for both AA. At this dose, Met and Leu independently evoked higher protein levels of MRCKα, sterol regulatory element-binding protein 1 (SREBP1) and Cyclin D1, and increased phosphorylation of mTOR (Ser2448) and of phosphatidylinositol 3-kinase (PI3K) (Tyr317) after 24 h. The stimulatory effect of both AA on β-casein and on phosphorylation of mTOR and of protein kinase B (AKT) (Thr308), was blocked by silencing MRCKα expression, whereas that on phosphorylation of PI3K remained intact. Inhibiting PI3K with LY294002 blocked Met- and Leu-induced protein expression of MRCKα and β-casein and phosphorylation of mTOR. Overexpression of MRCKα increased protein levels of β-casein and phosphorylation of mTOR, which was prevented by AKT inhibitor MK2206.
Conclusion
Our results indicate that MRCKα is a key mediator of the Met- and Leu-induced signaling cascade, acting downstream of PI3K and upstream of AKT to control β-casein synthesis in BMEC.
Spatial Effects of Tourism development on Economic Resilience: An empirical study of Wenchuan earthquake based on dynamic spatial Durbin model
Authors: Zhang jun 
Published: April 2022

Abstract
This paper highlights the importance of tourism spatial effects. Using the panel data of 135 Wenchuan earthquake-affected counties which from 2008 to 2018, this study employs the dynamic spatial Durbin model to examine the spatial effects of tourism development on post-disaster economic resilience and compares the difference of tourism-growth nexus between severe disaster-affected counties and general disaster-affected counties. The empirical results show that tourism development contributes to economic resilience for general disaster-affected counties, which supports the tourism-led growth hypothesis, whereas there is an inverted U-shape relationship for severe disaster-affected counties. Furthermore, the spatial spillover effects of tourism are insignificant. Plausible explanations of these results are discussed and policy suggestions are provided.
Gastrointestinal helminths in horses in Serbia and various factors affecting the prevalence
Authors: Ilić Tamara , Bogunović Danica , Nenadović Katarina , Gajić Bojan , Dimitrijević Sanda , Popović Gorana , Kulišić Zoran , Milosavljević Petar 
Published: April 2022

Abstract
The aim of this study was an epizootiological screening and determination of parasitological state of horses in the territory of the Republic of Serbia. In the period from April 2017 to December 2018, parasitological examination of 548 samples from horses of different breed, age and sex from four regions and 18 districts of the country was performed. Coprological diagnostic was done by using qualitative methods without concentration and qualitative methods with concentration of parasitic elements. Quantification of obtained results was performed using semi-qualitative faecal egg count. Four helminthoses were detected in the examined samples: P. equorum (8.57%), O. equi (3.65%), strongylid eggs (71.17%) and Anoplocephala spp. (0.91%). The total prevalence of helminthoses was 77.19%. Monoinfections were significantly more present 70.07% compared to coinfections (7.12%). Obtained results are of great contribution to clinical parasitology and pathology, especially from the aspect of animal health, welfare and preservation of horse population.
Aquatic Toxicity of Hydroquinone and Catechol Following Metal Oxide Treatment to Ceriodaphnia dubia and Pimephales promelas
Authors: Abugazleh Mohd Kotaiba , Ali Hashim M. , Chester Jae A. , Bouldin Jennifer L. 
Published: April 2022

Abstract
Metal oxides comprise a large group of chemicals used in water treatment to adsorb organic pollutants. The effects of titanium dioxide (TiO2) and iron (III) oxide (Fe2O3) to reduce the chronic toxicity of (phenolic) C6H6(OH)2 isomers, namely hydroquinone (HQ) and catechol (CAT) to Ceriodaphnia dubia and Pimephales promelas were investigated. The toxic endpoints following metal oxide treatment were compared to endpoints of untreated CAT and HQ. Chronic toxicity testing to HQ resulted in greater toxicity than CAT for both test organisms; the median lethal concentrations (LC50) for CAT were 3.66 to 12.36 mg.L− 1 for C. dubia and P. promelas, respectively, while LC50s for HQ were 0.07 to 0.05 mg.L− 1, respectively. Despite both treated solutions presented lower toxic endpoints than those in the untreated solutions, Fe2O3 had a better potential to reduce the toxic effects of CAT and HQ than TiO2.
Dynapenia of Ankle Muscles Negatively Affects Static Balance in Old Age
Authors: Skeparianos Alexandros , Amiridis Ioannis , Violaris Petros , Tsabalaki Zoi , Salonikidis Konstantinos , Negro Francesco , Hatzitaki Vassilia 
Published: April 2022

Abstract
Background
The maintenance of balance and mobility is essential in preserving physical capacities and daily activities in elderly. The assessment of potential contributors causing poor balance and mobility could help to identify people with an increased risk of sustaining injuries and falls. Previous research have found inconsistent findings regarding the relatinship between the lower-extremity muscle strength and postural control. Therefore, the purpose of the present study was to examine the relationship between ankle muscles strength and postural tasks of increasing difficulty in elderly.
Methods
Forty-three elderly adults (20 women) performed maximal isometric plantarflexions and dorsiflexions and 3 postural tasks: normal quiet stance (60s), tandem stance (20s), and one-legged stance (10s) on a pressure platform. Postural sway was quantified using center of pressure velocity, total path, sway area, sway ellipse, peak-to-peak amplitude and standard deviation of center of pressure displacement in anterior/posterior and medio/lateral direction. The weight distribution was also quantified as an index of asymmetry in both directions. Surface electromyographic (EMG) activity of the Tibialis Anterior, Medial Gastrocnemius, Rectus Femoris and Semitendinosus was recorded during posture.
Results
Across balance tasks, the greater the posture variables, the less the isometric plantar and dorsiflexion torque (p < 0.001). When narrowing the base of support, all postural variables and EMG activity increased significantly (p < 0.01), the increase was greater in ankle muscles than in hip muscles (p < 0.0005). During normal quiet stance (r = .683) and tandem stance (r = .641), the more symmetrical the weight distribution between the two legs, the higher the center of pressure velocity.
Conclusion
It is concluded that ankle muscles strength, could be considered a reliable predictor of the static balance control, especially when postural requirements relate to a narrower stance. Hence, the improvement of plantar flexors and dorsiflexors strength, but not the pursuit of an absolute symmetry of weight distribution, may be particularly beneficial for improving static balance control in old age.