bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of Lyophilized Growth Factors Injection for Subacromial Impingement Syndrome: A Prospective Randomized Double-Blind Placebo Controlled Study
Authors: El-Sherif Sherine Mahmoud , Moustafa Mowaffak , Ashraf Jailan , Fahmy Hossam , Abdel-naby hoda 
Published: August 2022

Abstract
ObjectiveEvaluate efficacy of platelet-derived lyophilized growth factors (L-GF) injection versus placebo in treatment of subacromial impingement (SIS).MethodsSixty patients with SIS (clinically and sonographically) were included in this randomized double blind placebo controlled study. Patients were randomly divided to receive either saline (group 1) or L-GF (group 2) injection, under US-guidance. Clinical examination, Visual analogue scale of pain, Shoulder Pain and Disability Index (SPADI), and shoulder ultrasound were done before and at 8th week after injection. ResultsFollow up assessment showed statistically significant improvement in L-GF group as regards active flexion & active and passive internal rotation and extension, SPADI-Disability scale, VAS of pain as well as thickness of the supraspinatus tendon by US. Concerning painful arc, regression analysis has shown that placebo group is about 30 times more than the L-GF group to get painful arc at follow up. On the other hand, both groups showed statistically significant improvement regarding SPADI-Pain scale and SPADI-Total as well as passive flexion & abduction (still mean value of abduction was significantly higher in LG-F group). ConclusionsL-GF injection in patients with SIS has resulted in significant improvement as regards pain and disability according to the improvement in shoulder range of movement, painful arc sign, VAS, SPADI, as well as significant reduction in the thickness of the supraspinatus tendon as measured by ultrasound compared to the saline group. Trial registration: Identification number is NCT04330027, date of first registration (01/04/2020)Unique on 21/11/ 2019, Protocol ID: 0106178,
Involvement of the nucleolar protein Nucleophosmin (NPM1) in Human Respiratory Syncytial Virus replication
Authors: Barbosa Ana Beatriz Ruiz Afonso , de Oliveira Andressa Peres , Silva Fernando Riback , Pavan Isadora Carolina Betim , Simabuco Fernando Moreira , Eléouët Jean-François , Ventura Armando Morais 
Published: August 2022

Abstract
Human respiratory syncytial virus (hRSV) accounts for most lower respiratory tract infections during infancy and childhood. We previously demonstrated that the hRSV Matrix (M) protein interacts with the nucleolar protein Nucleophosmin 1 (NPM1) in HEK 293T cells. In this study we confirmed this interaction by using an in vitro pull-down assay. Knockdown of NPM1 with shRNA against NPM1 mRNA as well as the use of an NPM1 oligomerization inhibitor, affected hRSV proteins expression. These results indicate that the association of NPM1 with M is important for hRSV replication and is a possible target for antiviral strategies.
Bee venom induces the interaction between phosphorylated histone variant, γH2AX, and intracellular location of beta actin in cancer cells
Authors: TETİKOĞLU Sinan , Uzuner Selcen Celik 
Published: August 2022

Abstract
Bee venom is a natural compound and candidate anti-cancer agent with selective cytotoxic effect on some cancer cells. However, the cellular mechanisms of how bee venom selectively targets cancer cells remain elusive. The aim of this study was to reveal the genotoxic effect of bee venom in concordance with the location of β-actin protein throughout the nucleus or/and cytoplasm. For this aim, the level of H2AX phosphorylation (γH2AX) and intracellular location of β-actin were assessed by immunofluorescence in liver (HEPG2) and metastatic breast (MDA-MB-231) cancer cell lines compared to normal fibroblasts (NIH3T3) after bee venom. Co-localisation of γH2AX and β-actin were also analysed. The results showed that the levels of γH2AX staining decreased in normal cells but increased in cancer cells. Majority of β-actin was localised within the cytoplasm of normal cells after bee venom, but it was mostly accumulated within the nucleus in cancer cells. Co-localisation of β-actin and γH2AX both in nucleus and cytoplasm was induced in each cell by different patterns. The results showed that normal and cancerous cells had different responses against bee venom, and suggested that bee venom induced a cellular response by the interaction between γH2AX and β-actin.
The effects of cryopreservation on follicular fluid samples in patients with Polycystic Ovary Syndrome
Authors: Zhou Dong-Jie , Lu Jun , Jin Xin , Jiang Min , Xue Tong-Min , Gao Ling-ling , Zhang Xiao-mei , Liu Wan-jun , Wang Li-ping 
Published: August 2022

Abstract
PurposeTo investigate the quality control of follicular fluid samples from polycystic ovary syndromepatients under -80 ℃ cryopreservation, explore the effects of cryopreservation in differentperiods.MethodsSeventy-five samples were selected to evaluate the quality of PCOS samples stored in a -80°C refrigerator at the Northern Jiangsu People’s Hospital during different time periods (from 2017 to 2018) by DNA integrity determination, RNA integrity determination and housekeeping gene expression.ResultsThe samples of PCOS follicles were divided into groups to be tested at five stages, with each stage lasting four months. The DNA quality of all the samples was high and without significant degradation in the majority of samples (97%). Samples had high RNA quality, and they fully satisfied the scientific research requirements with RINs≥6.ConclusionThe majority of samples had high molecular quality and were suitable for the requirements of downstream experiments. This study provides a reference for DNA and RNA extraction from different PCOS samples preserved for different amounts of time for scientific research.
Effects of Depression, Anxiety, and Pain Catastrophizing on Total Hip Arthroplasty Patient Activity Level
Authors: Reine Seth , Xi Yin , Archer Holden , Wells Joel 
Published: August 2022

Abstract
Background: Psychological factors have a strong association of improvement with increased physical activity in the general population. The effects of depressive symptoms, anxiety, and pain catastrophizing on activity level have not been thoroughly explored in patients undergoing total hip arthroplasty (THA), which is often performed in response to pain, functional limitations, and activity limitations. Mental health significantly influences patient perspective on treatment success and quality of life. We hypothesize that improvement in screenings for depression, anxiety, and catastrophizing will correlate with improvement in activity levels between preoperative and postoperative times.Methods: Two hundred ninety-two patients (313 hips) who underwent THA with a minimum one-year (mean 615±270 days) follow-up completed preoperative and postoperative surveys containing UCLA Activity Score, Hospital Anxiety Depression Scale (HADS), Pain Catastrophizing Scale (PCS), and Depression Anxiety Stress Scale-21 (DASS). Wilcoxon Signed-rank tests were performed between preoperative and postoperative times for each outcome measure and their subscales. Partial Spearman rank-order correlations were performed between change in UCLA Score and change in HADS, PCS, and DASS subscales controlling for age, BMI, follow-up time, and preoperative subscale score with false discovery rate correction.Results: Wilcoxon tests found significant improvements in UCLA Score (p-value<0.0001) and every subscale of PCS, HADS, and DASS (p-values<0.0001). Significant negative correlations existed between change in UCLA Score and change in HADS-anxiety (rs=-0.21,p-value<0.001), change in HADS-depression (rs=-0.23,p-value<0.001), and change in DASS-anxiety (rs=-0.22,p-value=0.004) following THA. Weaker significant negative correlations existed between change in UCLA Score and change in the remaining PCS (p-values=0.006) and DASS-depression (p-value=0.037) subscales following THA.Conclusion: Improvements in patient-reported depression, anxiety, and pain catastrophizing screenings following THA were associated with increased activity levels. Patients screening for catastrophizing, depression, and anxiety achieved statistically and clinically meaningful improvements in symptoms following THA. Addressing patient mental health may provide another avenue in the holistic care of THA patients.
Clustering method for generic analysis of histological fibrosis staining – an open-source tool
Authors: Remes Anca , Noormalal Marie , Schmiedel Nesrin , Frey Norbert , Frank Derk , Müller Oliver J. , Graf Markus 
Published: August 2022

Abstract
Pathological remodeling of the extracellular matrix is a hallmark of cardiovascular disease, causing cardiac dysfunction by reducing ejection fraction and impaired electrical conductance, leading to arrythmias. Hence, accurate quantification of fibrosis deposition in histological sections is of extreme importance for preclinical and clinical studies. Current automatic tools do not perform well under variant conditions. Moreover, users do not have the option to evaluate data from staining methods of their choice according to their purpose. To overcome these challenges, we underline a novel machine learning-based tool (FibroSoft) and we show its feasibility in a model of cardiac hypertrophy and heart failure in mice. Our results demonstrate that FibroSoft can identify fibrosis in diseased myocardium and the obtained results are user-independent. In addition, the results acquired using our software strongly correlate to those obtained by Western blot analysis of collagen 1 expression. Additionally, we could show that this method can be used for Masson’s Trichrome and Picosirius Red stained histological images. The evaluation of our method also indicates that it can be used for any particular histology segmentation and quantification. In conclusion, our approach provides a powerful example of the feasibility of machine learning strategies to enable automatic analysis of histological images.
Realtime user ratings as a strategy for combatting misinformation: An experimental study
Authors: Stein Jonas , Frey Vincenz , de Rijt Arnout van 
Published: August 2022

Abstract
Fact-checking takes time. As a consequence, verdicts are usually reached after a message has started to go viral and interventions can have only limited effect. A new approach inspired by the scholarly debate and implemented in practice is to harness the wisdom of the crowd by enabling recipients of an online message to attach veracity assessments to it, with the intention to allow poor initial crowd reception to temper belief in and further spread of misinformation. We study this approach by letting 4,000 subjects in 80 experimental bipartisan communities sequentially rate the veracity of informational messages. We find that in well-mixed communities, the public display of earlier veracity ratings indeed enhances the correct classification of true and false messages by subsequent users. However, crowd intelligence backfires when false information is sequentially rated in ideologically segregated communities. This happens because early raters’ ideological bias, which is aligned with a message, influences later raters’ assessments away from the truth. These results suggest that network segregation poses an important problem for community misinformation detection systems that must be accounted for in the design of such systems.
Overexpression of ZmDUF1644 from Zoysia matrella enhances salt tolerance in Arabidopsis thaliana
Authors: Li Guosi , Yin Qihui , Chen Yuting , Li Xiaoxue , Chen Xuelei , Deng Hui , Zhu Fucheng , Wei pei pei 
Published: August 2022

Abstract
The DUF1644 family is a highly conserved and functionally unknown protein family and has been demonstrated significantly improved the salt tolerance of rice (nonhalophytes). However, little is understood about the function of the DUF1644 gene in halophytes. Further studies are required to determine whether it has a similar function in halophyte species. In our study, the functions of ZmDUF1644 from halophyte Zoysia matrella Z123 species were investigated under salt stress. Phylogenetic analysis clustered ZmDUF1644 and OsSDIP361 in the same group, which function as a salttolerant genes in rice. Our experimental data suggested that the ZmDUF1644 gene significantly alleviated the growth inhibition of transgenic Arabidopsis thaliana seedlings caused by salt stress. The maximum root length, fresh weight, and photosynthetic pigment content of transgenic seedlings were obviously better than that of the wild type. Moreover, a relatively lower relative electrolyte leakage rate, toxic ion (Na+) content, and Na+/K+ ratios were also found in the transgenic line when compared with the wild type under salt stress. In conclusion, this study revealed that the ZmDUF1644 gene was overexpressed in Arabidopsis thaliana significantly enhancing the salt tolerance of seedlings. It is the first report on the functions of ZmDUF1644 from halophyte Zoysia matrella which has a similar function in halophyte species.
Population mixing mediates the intestinal flora composition and facilitates invasiveness in a globally invasive fruit fly
Authors: Wang Yidan , Li Zhihong , Zhao Zihua 
Published: August 2022

Abstract
Background
Changes in population heterozygosity and genetic diversity play important roles in mediating life history traits of organisms; these changes often lead to phenotypic evolution in offspring, which become superior to their parents. In the present study, we examined phenotypic differentiation, the intestinal microbiome composition and metabolism in the oriental fruit fly (Bactrocera dorsalis) by comparing an inbred (monophyletic) native population and an outbred (mixed) invasive population.
Results
The results showed that the outbred population of B. dorsalis had significantly higher biomass, adult longevity, and fecundity than the inbred population. Additionally, intestinal microflora analysis revealed that both Diutina rugosa and Komagataeibacter saccharivorans were significantly enriched in the outbred population with higher genetic heterozygosity. D. rugosa enrichment altered amino acid metabolism in the intestinal tract, and supplementing essential amino acids (e.g., histidine and glutamine) in the diet led to an increase in pupal weight of the outbred population. Additionally, transcriptome analysis revealed that the HSPA1S gene was significantly downregulated in the outbred population. HSPA1S was involved in activation of the JNK-MAPK pathway through negative regulation, caused the upregulation of juvenile hormone (JH), and led to an increase in biomass in outbred flies.
Conclusion
In conclusion, the outbred population had an altered intestinal microbe composition, mediating metabolism and transcriptional regulation, leading to phenotypic differentiation; this may be a potential mechanism driving the global invasion of B. dorsalis. Thus, multiple introductions could lead to invasiveness enhancement in B. dorsalis through population mixing, providing preliminary evidence that changes in the intestinal microbiome can promote biological invasion.
Impact of serum cystatin C level on long-term cognitive impairment after acute ischemic stroke and transient ischemic attack
Authors: Zuo Lijun , Dong YanHong , Pan Yuesong , Yan Hongyi , Meng Xia , Li Hao , Zhao Xingquan , Wang Yilong , Wang Yongjun , Liao MD；Xiaoling 
Published: August 2022

AbstractObjective:Cognitive impairment after stroke/transient ischemic attack (TIA) has a high prevalence. Cystatin C (CysC) has been found as a novel biomarker of neurodegenerative diseases, such as dementia and Alzheimer’s disease. We aimed to explore the possible correlations of serum cystatin C level with cognitive impairment in patients who had mild stroke and TIA after 1 year.Methods:We measured serum CysC levels in 1025 participants with a minor ischemic stroke/TIA from enrolled from the Impairment of Cognition and Sleep (ICONS) study of the China National Stroke Registry-3 (CNSR-3). They were divided into four groups according to quartiles of baseline CysC levels. Patients’ cognitive functions were assessed by MoCA-Beijing at day 14 and at 1 year. Multiple logistic regression models were performed to evaluate the relationship between CysC and PSCI at 1 year follow-up.Results: Cognitive impairment was defined as MoCA-Beijing ≤22. Most patients were in 60s (61.52±10.97 years old) with a median (interquartile range) National Institute of Health Stroke Scale score of 3.00(4.00) and greater than primary school level of education, and 743 participants (72.49%) were male. Among the 1025 participants, 331 participants (32.29%) patients suffered PSCI at 1 year follow-up. A U-shaped association was observed between CysC and 1-year PSCI [quartile (Q)1 vs. Q3: adjusted odds ratio (aOR) 2.64, 95% CI 1.65-4.20, p＜0.0001; Q2 vs. Q3: aOR 1.83, 95% CI 1.17-2.84, p = 0.0078; Q4 vs. Q3: aOR 1.86, 95% CI 1.20-2.87, p = 0.0055]. Moreover, the U-shaped trends were also found between CysC level and the subscores of attention, recall, abstraction and language in MoCA.Conclusions: CysC showed a U-shaped correlation with 1-year overall cognitive function. It is probable that measurement of the serum cystatin C level would aid in the early diagnosis of PSCI.
The changes of distance between nipples following correction of women pectus excavatum
Authors: Yoo Gyeol , Jeon Hui Hyung , Rha Eun Young , Ko Jun Gul , Baek Sang Oon , Lee Jun Yong , Jeong Jin Yong 
Published: August 2022

Abstract
The breasts in women pectus excavatum patients frequently appear to be slanting medially along the inclination of the distorted ribs. This study aims to evaluate changes in the distance between the nipples and to find out whether medially slanting breasts are corrected in women pectus excavatum patients following modified Nuss procedure. This case series analysis enrolled 22 young women patients with pectus excavatum between October 2011 and September 2020. We measured all the patients’ distances from the sternal midline to the right and left nipples, based on chest computerized tomography. We calculated the distances between nipples as being the sum of the right and left distances. The mean age of patients was 16.50 ± 4.73 years, and the follow-up periods were 35.59 ± 20.23 months. The postoperative Haller indices (2.89 ± 0.43) were significantly lower than the preoperative Haller indices (5.14 ± 1.96) (p = 0.000). The distances between the nipples before and after Nuss procedure were 145.17 ± 17.73 mm and 172.29 ± 19.11 mm, which is a significant increase following surgery. (p = 0.000). Our results demonstrated that skeletal correction with modified Nuss procedure in pectus excavatum increased the distance between nipples, indicating that medially slanting breasts had been corrected.
Multiple plasma membrane reporters discern LHFPL5 region that blocks trafficking to the plasma membrane
Authors: Soler David , Ballesteros Angela , Sloan Andrew , McCormick Tom , Stepanyan Ruben 
Published: August 2022

Abstract
The mechanotransduction (MET) channel of the inner ear sensory receptor cells, termed hair cells, is a central protein complex that enables our senses of hearing and balance. Hair cell MET requires an elaborate interplay of multiple proteins that form the MET channel. One of the MET complex components is the transmembrane protein LHFPL5, which is required for hair cell MET and hearing. LHFPL5 is thought to interact with two other MET channel complex proteins, PCDH15 and TMC1. Despite localizing to the plasma membrane of stereocilia, the mechanosensing organelles of hair cells, LHFPL5 requires its binding partner within the MET complex, PCDH15, to localize to the stereocilia tips in hair cells and to the plasma membrane in heterologous cells. Using the Aquaporin 3-tGFP reporter (AGR) for plasma membrane localization, we found that a region within extracellular loop 1, which interacts with PCDH15, precludes the trafficking of the AGR reporter to the plasma membrane of HEK293 cells. Our results suggest that the presence of protein partners may mask endoplasmic reticulum retention regions or enable the proper folding and trafficking of the MET complex components, to facilitate expression of the MET complex at the stereocilia membrane.
Detection method of rail surface defects based on deep learning
Authors: Xu Jianming 
Published: August 2022

Abstract
For the detection of uneven and irregular texture surface defects, the conventional image reconstruction is difficult because the background texture is a nonstationary signal. At the same time, because it is difficult to obtain the image feature information such as the shape and gray level of defects in advance, and the visual features of similar defects can show great dispersion, the existing detection methods that rely on target features are difficult to apply. According to the vibration theory and transient finite element-boundary element theory, a method of analyzing and detecting rail fracture defects by using rail vibration response data is proposed. By establishing the dynamic model of vibration analysis of ballastless track, the characteristics of vibration acceleration of track under the condition of complete rail fracture and 50% fracture of rail section under the periodic load excitation of nodes are studied. In this paper, an unsupervised learning surface defect detection method based on image inpainting is proposed.In this method, a small number of normal texture sample images should be used as training sets to train the network model. Then, during detection, the missing region is artificially set in the sample image, and the content of the missing region is predicted by using the network model. Finally, according to the structural similarity evaluation and residual error between the reconstructed image and the image to be tested, the defect detection and separation are realized. Experimental results show that the algorithm has strong generalization ability, can accurately segment defects of tile images with large amount of complex texture, uneven illumination and low contrast, and has strong robustness.
Cortical Microtubule Dynamics During Reaction Wood Formation Ensures Context-Appropriate Cellulose Microfibril Angle in Woody Trees
Authors: Tobias Larissa Machado , McFarlane Heather E. , Bossinger Gerd , Spokevicius Antanas Vytas 
Published: August 2022

Abstract

Microtubules are directly and indirectly involved in guiding cellulose synthase complexes (CSCs) through the plasma membrane. The angle of cellulose deposition is a critical response to environmental signals and/or stress conditions, and particularly crucial during reaction wood formation, a process in which woody plants deposit additional cell wall material to counteract gravitational forces. Tubulin genes are upregulated in response to gravitational stimulus during reaction wood formation, which can result in changes to microtubule assembly. In this study, microtubules were visualised in three woody tree species (two angiosperms: Eucalyptus globulus Labill., Populus alba L., and one gymnosperm: Pinus radiata D. Don.) using immunofluorescence to quantitatively evaluate microtubule organisation during reaction wood formation. Our results suggest that reorientation of the cortical microtubule array affects secondary cell wall deposition, even across different types of reaction wood, by ensuring context-appropriate orientation of cellulose microfibrils and determining MFA in wood cells. Pharmacological studies conducted on in vitro cultured stem segments or in vivo during reaction wood formation corroborated these important roles for microtubules during wood development. This study starts to unveil the role of tubulins during wood formation by exploring cortical microtubule array organisation in trees subjected to gravitational stimulus and it sheds light on cellular and molecular mechanisms behind cellulose deposition in tree species.