bluuebunny/crossref_metadata_2025_split huggingface dataset:
An integrative bioinformatics investigation and experimental validation of Chromobox family in Diffuse large B-cell lymphoma
Authors: Zhou Fenling , Chen Lu , Lu Peng , Cao Yuli , Deng Cuilan , Liu Gexiu 
Published: December 2022

Abstract
Background
Diffuse large B-cell lymphoma (DLBCL) is one of the most aggressive malignant tumors. Chromobox (CBX) family plays the role of oncogenes in various malignancies.
Methods
The transcriptional and protein levels of CBX family were confirmed by GEPIA, Oncomine, CCLE, and HPA database. Screening of co-expressed genes and gene function enrichment analysis were performed by GeneMANIA and DAVID 6.8. The prognostic value, immune cell infiltration and drug sensitivity analysis of CBX family in DLBCL were performed by Genomicscape, TIMER2.0 and GSCALite database. Confirmatory Tests of CBX family protein expression in DLBCL were performed by immunohistochemistry.
Results
The mRNA and protein expression of CBX1/2/3/5/6 were higher in DLBCL tissues than control groups. Enrichment analysis showed that the functions of CBX family were mainly related to chromatin remodeling, methylation-dependent protein binding, and VEGF signaling pathway. The high mRNA expression of CBX2/3/5/6 was identified to be associated with short overall survival (OS) in DLBCL patients. Multivariate COX regression indicated that CBX3 was independent prognostic marker. Immune infiltration analysis revealed that the mRNA expression of CBX family (especially CBX1, CBX5, and CBX6) in DLBCL was significantly correlated with the infiltration of most immune cells (including B cells, CD8 + T cells, CD4 + T cells, neutrophils, monocytes, macrophages, and Treg cells). Meanwhile, there was a strong correlation between the expression levels of CBX1/5/6 and surface markers of immune cells, such as the widely studied PVR-like protein receptor/ligand and PDL-1 immune checkpoint. Notably, our study found that DLBCL cells with CBX1 overexpression was resistant to the common anti-tumor drugs, but CBX2/5 had two polarities. Finally, we confirmed the higher expression of CBX1/2/3/5/6 in DLBCL tissues compared with control groups by immunohistochemistry.
Conclusion
We provided a detailed analysis of the relationship between the CBX family and the prognosis of DLBCL. Distinguished from other studies, We found that high mRNA expression of CBX2/3/5/6 was associated with poor prognosis in DLBCL patients, and Multivariate COX regression indicated that CBX3 was independent prognostic marker. Besides, our study also found an association between the CBX family and antitumour drug resistance, and provided a relationship between CBX family expression and immune cell infiltration.
Ten years of gross alpha/beta measurement interlaboratory comparisons in drinking water
Authors: Yin Liangliang , Xie Yuhan , Qian Yuxin , Ji Yanqin 
Published: December 2022

Abstract
Interlaboratory comparison exercises for determination of activity concentration of gross alpha and gross beta in drinking water organized by the National Institute for Radiological Protection (NIRP), China CDC, have been carried out since 2011 to 2021. The purpose of this work was to assess the accuracy and precision of low level radioactivity concentration for gross alpha and gross beta analysis. The comparisons used the natural water samples and the performance of participating laboratories was evaluated with respect to the reference values using Z-score performance indicator. Over the years, the percentage of laboratories with acceptable results was 80%-92%, the comparison of the acceptable results have increased. The non-acceptance results are mainly due to the uncertainty caused by low detection efficiency and short measuring time.
Ambient pressure storage of high-density methane in carbon nanopores having thermally switchable graphene-locks
Authors: Wang Shuwen , Vallejos-Burgos Fernando , Furuse Ayumi , Marco-Lozar Juan P. , Nagae Miu , Tanaka Hideki , Kanoh Hirofumi , Albero Joaquin Silvestre , Hayashi Takuya , Kaneko Katsumi 
Published: December 2022

Abstract
Physisorption of methane in porous solids has been actively studied to achieve high methane storage capacity, but it requires heavy vessels due to the high pressures involved. In addition, physisorption of methane is quite sensitive to temperature elevation, even near ambient conditions. We developed a method for high-density methane storage at ambient pressure by installing pore-entrance locks controlled by waste heat at 473 K. Under ambient conditions, the entrance locks are stable enough to store a large amount of highly pressurized methane in the carbon nanopores at 19.9 MPa and can be unlocked at 473 K for encapsulation and release of methane. This novel ambient pressure storage method for pressurized methane enables storage of more methane than any other adsorbent at 3.5 MPa and 298 K. The waste-heat open/close pore-entrance locking mechanism supports long-term storage of a renewable energy source and provides a stationary energy supply.
GIS-Based Analytical Hierarchy Process (AHP) for Flood Hazards and Risks Assessment: A Case Study of the Port City of Montego Bay and the surrounding St. James Parish, Jamaica
Authors: McKenzie Ricordo , Gala Tekleab 
Published: December 2022

Abstract
A flood is a hydrological phenomenon of a significant surge in the runoff water that overwhelms and overflows a drain, river, or stream, usually after heavy rainfall, tropical storms, and cyclones. When it occurs, it causes adverse impacts on human life and public health, domestic animals, properties, and croplands. The Caribbean island of Jamaica is located in the geographical area where the current and forecasted hydrological, physical, climatic, and human conditions are set for probable flood hazard occurrences. Therefore, this study aimed at modeling the likelihood of flood hazard occurrences, and risk assessment for Saint James Parish, Jamaica, and its port city of Montego Bay using the Geographic Information Systems (GIS)-based analytical hierarchy process of multicriteria analysis. Accordingly, 36% of the study area is assessed to experience a very high and high probability of flood hazard occurrences. On the other hand, 25% of the study area is subjected to risks of high and very high exposure and vulnerability to the hazard. Wetlands are the most adversely impacted land-use and landcover types (i.e., 100%) followed by Built-up areas (i.e., 74%) and agricultural croplands (24%), indicating the unique vulnerability of the area to the hazards, and damages thereof.
Titania Nanotube Arrays Surface Anodization and Various Human Cell Line Models Adherence Profiles
Authors: Mydin Rabiatul Basria S. M. N. , Effendy Wan Nuramiera Faznie Wan Eddis , Hazan Roshasnorlyza , Romli Nur Afiqah Amalina , Shaharuddin Sadiqah , Khan Ibrahim Hussin , Sreekan Srimala 
Published: December 2022

Abstract
Titania nanotube arrays (TNA) have high biocompatibility, less toxicity, and a large surface area per volume; thus, TNA offer great potential in biomedical applications. Interactions between micro environment and cell on the TNA surface are intensively investigated regarding cell attachment and interaction. Anodization was used to create a highly ordered nano-porous oxide layer with nano-sized pores on the surface of the titanium. This process was carried out at 30 V with a sweep rate of 1 V/sec for a different duration (10 s, 1 min, 2 min, 5 min, 10 min, 20 min, 30 min, 1 h, 2 h, 3 h and 6 h). On an anodized titanium surface, the cell adhesion of several adherence cells was observed using a field emission scanning electron microscope (FESEM). Due to its important role in controlling the morphology of the nanotube structures, extending anodization time causes the length of the nanotubes increases. Hence, the optimised anodization time on the TNA surface at 30 V with a sweep rate of 1 V/s directly impacts cell adhesion after incubating for 48 h. The anodic potential of TNA was successfully obtained at 30 V with a sweep rate of 1 V/sec for 30 min, which could modulate diverse cellular responses of cell adhesion observed by FESEM.
swnK plays an important role in the biosynthesis of swainsonine in Metarhizium anisopliae
Authors: Huang Enxia , Zhang Yu , Sun Lu , Zhu Yiru , Tang Shiyu , Mo Chonghui , Zhao Baoyu , lu hao 
Published: December 2022

Abstract
Swainsonine (SW) is the principal toxic ingredient of locoweeds, and is produced by fungi including Metarhizium anisopliae, Slafractonia leguminicola, and Alternaria oxytropis. A key enzyme in the SW synthesis pathway is a hybrid SWNK/NRPS. The downstream biosynthetic pathways of SW have been clearly analyzed by a knockout and complement of swnK in Metarhizium robertsii, but the upstream steps and the associated regulatory proteins have not been thoroughly investigated. To verify the role of swnK in the SW biosynthesis pathway of M. anisopliae, the concentration of SW and the swnK expression in M. anisopliae fermentation from 1st to 7th d were determined using LC-MS and RT-qPCR, respectively. M. anisopliae had the highest SW content and swnK expression on the 5th day of fermentation; We obtained a mutant strain (MT) by PEG-mediated homologous recombination (HR) to knock out swnK in the wild-type (WT) strain. And we obtained a complemented-type (CT) strain by transforming a modified PUC19 complementation vector containing the geneticin (G418) resistance gene and swnK. SW was not detected in the MT strain and reverted to its original level in the CT strain; We also used a Psilent-1 plasmid with Benomyl (ben)-resistant that interfered with swnK of wild-type strain (WT). The level of SW in the RNAi strain was markedly diminished. RNAi of swnK affected the formation of the cell wall in M. anisopliae. These results indicate that swnK plays a crucial role in the SW biosynthesis of M. anisopliae.
Knowledge, Attitude and Practice Regarding the Factors and Management of Maternal Mortality Amongst the 2nd Year Degree and Diploma Nursing Students at a University Windhoek, Namibia
Authors: Kadhila Joseph Galukeni , Tjomombura Eveline 
Published: December 2022

Abstract
Background: Maternal death is a major health problem in Namibia as it is the case in most countries. The rate at which women are dying due to pregnancy related causes is high and the cause as well as risk factors are not well explored. Despite the introduction of several interventions by the Ministry of Health and Social Services to prevent the maternal deaths, according to the Namibian Demographic Health Survey, the maternal mortality ratio was 249 in 1992, 271 in 2000, 449 in 2006-2007 and 385 in 2013 respectively. It was against this background that the researcher conducted this to determine the knowledge, attitude and practice regarding the factors and management of maternal mortality amongst the 2nd year degree and diploma nursing students at a university, Windhoek, Namibia.
Aims: Assess the knowledge, attitude and practice of 2nd year degree nursing students and diploma nursing students at University regarding the factors and management of maternal mortality.
Objectives: This project is a report of a study to determine the knowledge and practice of 2nd year diploma and degree nursing students regarding factors and their management and to evaluate the attitude and assess the practice of the 2nd year diploma and degree nursing at a University regarding factors and management of maternal mortality
Methodology: In this study Quantitative approach was used so as to collect numerical data from the participants descriptive cross-sectional design was used to obtain data. Quantitative approach is the mathematical method of measuring and describing the observation of materials or characteristics. The target population of this study was the 2nd year diploma and degree student at the University of Namibia, main campus, Windhoek. Data was collected from the 13th of August 2022 to the 12th of October 2022 and was collected online using google forms. Researcher used probability sampling approach as a process of selecting the sample from the population. Descriptive statistics was used to determine the relationships between independent and dependent variables.
Results: A response rate of 54% was achieved. Out of 79 participants, 87.3% (n=69) were female and they were dominating while 12.7% (n=12) were male nursing students. All participants were Namibian. The majority of the participants had grade 12 certificate as the highest qualifications abstained. The study reveals that there was good display of knowledge, practice and attitude on the identification of factors related to maternal mortality.
Conclusion: lack of knowledge, practice and attitude on identification and proper management of factors associated with maternal mortality were revealed in the study. 34.6% of the participants failed to identify puerperal sepsis as an infection that can lead to maternal mortality while 65.4% were able to identify it correctly.
Improving Cotton Simulation Model, GOSSYM, for Soil, Photosynthesis, and Transpiration Processes
Authors: Beegum Sahila , Timlin Dennis , Reddy Kambham Raja , Reddy Vangimalla , Sun Wenguang , Wang Zhuangji , Fleisher David , Ray Chittaranjan 
Published: December 2022

Abstract
GOSSYM, a mechanistic, process-level cotton crop simulation model, has a two-dimensional (2D) gridded soil model called Rhizos that simulates the below-ground processes daily. Water movement is based on gradients of water content and not hydraulic heads. In GOSSYM, photosynthesis is calculated using a daily empirical light response function that requires calibration for response to elevated carbon dioxide (CO2). This report discusses improvements made to the GOSSYM model for soil, photosynthesis, and transpiration processes. GOSSYM's predictions of below-ground processes using Rhizos are improved by replacing it with 2DSOIL, a mechanistic 2D finite element soil process model. The photosynthesis and transpiration model in GOSSYM is replaced with a Farquhar biochemical model and Ball-Berry leaf energy balance model. The newly developed model (modified GOSSYM) is evaluated using field-scale and experimental data from SPAR (soil-plant-atmosphere-research) chambers. Modified GOSSYM better predicted net photosynthesis (root mean square error (RMSE) 25.5 versus 45.2 g CO2 m− 2 day− 1; index of agreement (IA) 0.89 versus 0.76) and transpiration (RMSE 3.3 versus 13.7 liters m− 2 day− 1; IA 0.92 versus 0.14) and improved the yield prediction by 6.0%. Modified GOSSYM improved the simulation of soil, photosynthesis, and transpiration processes, thereby improving the predictive ability of cotton crop growth and development.
Evaluation effect of recombinant lytic polysaccharide monooxygenases added to Aspergillus fumigatus var. niveus cocktail in saccharification of sugarcane bagasse
Authors: Gonçalves Aline Larissa , Cunha Paula Macedo , Lima Awana Silva , Santos Júlio César , Segato Fernando 
Published: December 2022

Abstract

Background: Lignocellulosic biomass is an advantageous alternative to produce biofuels, despite its recalcitrant characteristic. There are microorganisms in nature capable of efficiently degrade biomass, such as the filamentous fungi. Among them, Aspergillus fumigatus var. niveus (AFUMN) has a wide variety of carbohydrate-active enzymes (CAZymes), especially hydrolases, but a low number of oxidative enzymes in its genome. To confirm the enzymatic profile of this fungus, this work analyzed the secretome of AFUMN cultured in sugarcane bagasse as sole carbon source. As expected, the secretome showed a predominance of hydrolytic enzymes compared to the oxidative activity. However, it is known that hydrolytic enzymes act in synergism with oxidative proteins to efficiently degrade cellulose polymer, such as the LPMOs (Lytic Polysaccharide Monooxygenases).
Results: Thus, three LPMOs from the fungus Thermothelomyces thermophilus (TtLPMO9D, TtLPMO9H, and TtLPMO9O) were selected, heterologous expressed in Aspergillus nidulans, purified, and used to complement the AFUMN secretome aiming to evaluate the supplementation effect in the saccharification of sugarcane bagasse process. The saccharification assay was carried out using different concentrations of AFUMN secretome supplemented with recombinant T. thermophilus LPMOs, as well as ascorbic acid as reducing agent for oxidative enzymes.
Conclusions: Through a statistic design created by Design-Expert software, we were capable to analyze a possible cooperative effect between these components. The results obtained indicated that, in general, the addition of TtLPMO9D and ascorbic acid did not favor the conversion process in this study, while TtLPMO9O had a highly significant cooperative effect in bagasse saccharification compared to the control using only AFUMN secretome.
Knowledge, awareness, and worry about human monkeypox and attitude toward its vaccination in non endemic countries: a concise critical review
Authors: LOUNIS Mohamed 
Published: December 2022

Abstract
Background: In May 2022, the world has seen the emergence of human monkeypox, a new zoonotic viral disease in multiple non endemic countries. This health threat has been associated with high worry especially after the COVID-19 catastrophe. Also, the population is exposed to a huge amounts of information making them exposed to false information which could lead to embracing conspiracy theories. Thus evaluating the level of knowledge and the attitude toward vaccination are of great importance to raise them for the categories with low levels of knowledge and acceptance. This scoping review was conducted using PRISMA guidelines with the aim to evaluate the level of knowledge and awareness about monkeypox and the attitude toward its vaccination by selecting studies.
Results: The selection process allowed to include 16 studies in this review. These studies were conducted in 10 countries in Europe and Asia. 9 studies were exclusively related to monkeypox knowledge and awareness, 2 studies were exclusively related to monkeypox vaccines while 5 studies treated the two subjects. These studies were mainly conducted among healthcare workers or among the general population.
Results showed an unsatisfactory level of knowledge and awareness with some categories including physicians and aged individuals as the most informed. Regarding vaccination, Results showed that vaccine hesitancy is still common for both healthcare workers except among Chinese healthcare workers where the rate of vaccine acceptance was estimated at 90.1%.
Conclusions: This review could be helpful in the understanding of the knowledge and awareness and vaccines hesitancy in the first months of the emergence of the HMPX by comparing their evolution in the future studies.
Current status and factors influencing readiness to return to work among young and middle-aged stroke patients in China
Authors: Liu Ziwei , Li Jiaxin , Liu Fangli , Guan Ningxiao , Li Ye , Zhang Yu , Hou Linlin , Jiang Qiuhuan 
Published: December 2022

Abstract
Objectives: Stroke is affecting an increasing number of individuals of working age, and accurate assessment of the readiness to return to work can help to determine the timing of return to work and facilitate an early return to society. The objective of this study was to investigate the current state of readiness to return to work and the factors influencing it among young and middle-aged stroke patients in China.
Materials & Methods: A sample of young and middle-aged stroke patients hospitalized in a tertiary hospital in Henan Province between December 2021 and May 2022 were included in this study. A general information questionnaire and the Readiness to Return to Work Scale, Social Support Rating Scale, Stroke Rehabilitation Self-Efficacy Scale, and Fatigue Severity Scale were administered to the patients.
Results: Of the 203 patients successfully surveyed, 60 (29.6%) were in the pre-intention stage, 35 (17.2%) in the intention stage, 81 (39.9%) in the action-readiness-self-assessment stage, and 27 (13.3%) in the action-readiness-action stage. A logistic regression analysis showed that education level, monthly income, time to start rehabilitation exercise, social support, rehabilitation self-efficacy, and fatigue severity were the main factors affecting the readiness of young and middle-aged stroke patients to return to work.
Conclusion: The readiness of young and middle-aged stroke patients to return to work needs to be increased further. Healthcare professionals should consider the influencing factors of readiness to return to work and design targeted intervention programmes to facilitate successful return to work and normal life.
Study on Interaction and Binding Properties of Terpenes repellent active compounds with Odorant Binding Protein 9B in Tribolium castaneum
Authors: Sang Yu-Li , Qiang Xin-Yue , Dai Lu , Chen Li-Jiang , Liu Yu-Feng , Hao Yan-Jun , Liu Jing-Yu , Qi Jian-Zhong 
Published: December 2022

AbstractTo screen the odorant binding proteins (OBPs) ofTribolium castaneumrelated to repellent activity and explore the repellent mechanism of terpenoids by studying the interaction between ligands andTcOBPs. Behavior tests indicated that (1R)-(+)-α-Pinene andβ-Caryophyllene had repellent activity againstT. castaneum. Through homology modeling and molecular docking, we screenedTcOBP-9B, which is highly expressed in antennae and has strong interactions with (1R)-(+)-α-Pinene,β-Caryophylne, as target protein for recombinantly expressed. Fluorescence spectra and UV absorption spectra showed thatTcOBP-9B had strong bind to (1R)-(+)-α-Pinene andβ-Caryophyllene with concentration-dependent in static quenching. Thermodynamic date revealed that they formed stable complexes through van der Waals force, electrostatic and hydrophobic interactions. By fluorescence competitive binding assay, molecular docking and circular dichroism spectra, the binding sites ofTcOBP-9B was confirmed as theα-helix, where the ligands were competitively bound to 1-NPN. These results suggested thatTcOBP-9B was a significant target protein associated with repellent activity, providing new approach for screening repellents againstT. castaneum.
Mycorrhizal association controls soil carbon-degrading enzyme activities under nitrogen addition: Implications for soil carbon dynamics
Authors: Hu Yuanliu , Chen Ji , Olesen Jorgen , Groenigen Kees Jan van , Hui Dafeng , He Xinhua , Chen Guoying , Deng Qi 
Published: December 2022

AbstractIncreasing atmospheric nitrogen (N) deposition affects soil carbon (C) dynamics in terrestrial ecosystems. Recent evidence suggests that changes in C-degrading extracellular enzyme activities (C-EEAs) largely explain the responses of soil C dynamics to N deposition. However, the factors that control C-EEA variations under N addition remain unclear, impeding the inclusion of microbial mechanisms in global C cycle models. Using a global meta-analysis, we show that across a wide range of experimental and environmental factors, the response of C-EEAs to N addition is best predicted by mycorrhizal association. In ecosystems dominated by ectomycorrhizal plants, N addition stimulated cellulase activity by 24.4%, suppressed ligninase activity by 13.8%, and increased soil C stock by 5.8% on average. Nitrogen addition did not affect C-EEAs and soil C pool in ecosystems dominated by arbuscular mycorrhizal plants. Incorporating mycorrhizae in global C cycle models could improve predictions of soil C storage and stabilization under N enrichment.