bluuebunny/crossref_metadata_2025_split huggingface dataset:
No travel worsens depression: reciprocal relationship between travel and depression among older adults
Authors: Hyun Seungjae , Lee Yeonjin , Park Sangshin 
Published: August 2022

Abstract
Background
The aim of this study was to examine the bidirectional relationship between depression and travel.

Method
We analyzed 8524 participants’ data obtained from the 2008 to 2016 waves of the Korean Longitudinal Study of Ageing, a prospective cohort study. Depression was diagnosed using the Center for Epidemiological Studies Depression Scale: 10-Items (CES-D10), with scores of 4 or higher indicating depression. We used a generalized estimating equation and a cross-lagged panel model for statistical analysis.

Results
Participants who had not traveled for one year had a 71% higher risk of suffering from depression in the following year than did those who had traveled [relative risk (RR) = 1.71, P < 0.001], and participants with depression had more than double the increased risk of not traveling than did those not currently suffering from depression (RR = 2.08, P < 0.001). The cross-lagged panel model confirmed the vicious cycle involving the amount of travel and score on the CES-D10; individuals who traveled more frequently were more likely to have lower scores on the CES-D10 (coefficient = − 0.04 to − 0.03, Ps < 0.01), and individuals with higher scores were less likely to travel (coefficient = − 0.06 to − 0.03, Ps < 0.01).

Conclusions
The risk of depression increases for people who do not travel, and a reciprocal relationship exists between travel and depression.
Aboriginal medicinal plants of Queensland: ethnopharmacological uses, species diversity, and biodiscovery pathways
Authors: Turpin Gerry , Ritmejerytė Edita , Jamie Joanne , Crayn Darren , Wangchuk Phurpa 
Published: August 2022

Abstract
Background
Aboriginal peoples have occupied the island continent of Australia for millennia. Over 500 different clan groups or nations with distinctive cultures, beliefs, and languages have learnt to live sustainably and harmoniously with nature. They have developed an intimate and profound relationship with the environment, and their use of native plants in food and medicine is largely determined by the environment they lived in. Over 1511 plant species have been recorded as having been used medicinally in Australia. Most of these medicinal plants were recorded from the Aboriginal communities in Northern Territory, New South Wales, South Australia, and Western Australia. Not much has yet been reported on Aboriginal medicinal plants of Queensland. Therefore, the main aim of this review is to collect the literature on the medicinal plants used by Aboriginal peoples of Queensland and critically assess their ethnopharmacological uses.

Methods
The information used in this review was collected from archival material and uploaded into the Tropical Indigenous Ethnobotany Centre (TIEC) database. Archival material included botanist’s journals/books and old hard copy books. Scientific names of the medicinal plant species were matched against the ‘World Flora Online Plant List’, and ‘Australian Plant Census’ for currently accepted species names to avoid repetition. An oral traditional medical knowledge obtained through interviewing traditional knowledge holders (entered in the TIEC database) has not been captured in this review to protect their knowledge.

Results
This review identified 135 species of Queensland Aboriginal medicinal plants, which belong to 103 genera from 53 families, with Myrtaceae being the highest represented plant family. While trees represented the biggest habit, leaves were the most commonly used plant parts. Of 62 different diseases treated by the medicinal plants, highest number of plants are used for treating skin sores and infections. Few plants identified through this review can be found in other tropical countries but many of these medicinal plants are native to Australia. Many of these medicinal plants are also used as bush food by Aboriginal peoples.

Conclusion
Through extensive literature review, we found that 135 medicinal plants native to Queensland are used for treating 62 different diseases, especially skin infections. Since these medicinal plants are also used as bush food and are rarely studied using the Western scientific protocols, there is a huge potential for bioprospecting and bush food industry.
CDK7 inhibition augments response to multidrug chemotherapy in pancreatic cancer
Authors: Zeng Siyuan , Lan Bin , Ren Xiaofan , Zhang Shuman , Schreyer Daniel , Eckstein Markus , Yang Hai , Britzen-Laurent Nathalie , Dahl Andreas , Mukhopadhyay Debabrata , Chang David , Kutschick Isabella , Pfeffer Susanne , Bailey Peter , Biankin Andrew , Grützmann Robert , Pilarsky Christian 
Published: August 2022

AbstractBackgroundPancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer with a dismal prognosis. Although combined treatment with gemcitabine and albumin-bound paclitaxel has improved the prognosis of PDAC, both intrinsic and acquired chemoresistance remain as severe hurtles towards improved prognosis. Thus, new therapeutic targets and innovative strategies are urgently needed.MethodsIn this study, we used the KPC mouse model-derived PDAC cell line TB32047 to perform kinome-wide CRISPR-Cas9 loss-of-function screening. Next-generation sequencing and MAGeCK-VISPR analysis were performed to identify candidate genes. We then conducted cell viability, clonogenic, and apoptosis assays and evaluated the synergistic therapeutic effects of cyclin-dependent kinase 7 (CDK7) depletion or inhibition with gemcitabine (GEM) and paclitaxel (PTX) in a murine orthotopic pancreatic cancer model. For mechanistic studies, we performed genome enrichment analysis (GSEA) and Western blotting to identify and verify the pathways that render PDAC sensitive to GEM/PTX therapy.ResultsWe identified several cell cycle checkpoint kinases and DNA damage-related kinases as targets for overcoming chemoresistance. Among them, CDK7 ranked highly in both screenings. We demonstrated that both gene knockout and pharmacological inhibition of CDK7 by THZ1 result in cell cycle arrest, apoptosis induction, and DNA damage at least predominantly through the STAT3-MCL1-CHK1 axis. Furthermore, THZ1 synergized with GEM and PTX in vitro and in vivo, resulting in enhanced antitumor effects.ConclusionsOur findings support the application of CRISPR-Cas9 screening in identifying novel therapeutic targets and suggest new strategies for overcoming chemoresistance in pancreatic cancer.
Flow cytometry-based viability staining: an at-line tool for bioprocess monitoring of Sulfolobus acidocaldarius
Authors: Rastädter Kerstin , Tramontano Andrea , Wurm David J. , Spadiut Oliver , Quehenberger Julian 
Published: August 2022

AbstractDetermination of the viability, ratio of dead and live cell populations, of Sulfolobus acidocaldarius is still being done by tedious and material-intensive plating assays that can only provide time-lagged results. Although S. acidocaldarius, an extremophilic Archaeon thriving at 75 °C and pH 3.0, and related species harbor great potential for the exploitation as production hosts and biocatalysts in biotechnological applications, no industrial processes have been established yet. One hindrance is that during development and scaling of industrial bioprocesses timely monitoring of the impact of process parameters on the cultivated organism is crucial—a task that cannot be fulfilled by traditional plating assays. As alternative, flow cytometry (FCM) promises a fast and reliable method for viability assessment via the use of fluorescent dyes. In this study, commercially available fluorescent dyes applicable in S. acidocaldarius were identified. The dyes, fluorescein diacetate and concanavalin A conjugated with rhodamine, were discovered to be suitable for viability determination via FCM. For showing the applicability of the developed at-line tool for bioprocess monitoring, a chemostat cultivation on a defined growth medium at 75 °C, pH 3.0 was conducted. Over the timeframe of 800 h, this developed FCM method was compared to the plating assay by monitoring the change in viability upon controlled pH shifts. Both methods detected an impact on the viability at pH values of 2.0 and 1.5 when compared to pH 3.0. A logarithmic relationship between the viability observed via plating assay and via FCM was observed.
Changes of the liver metabolome following an intravenous lipopolysaccharide injection in Holstein cows supplemented with dietary carnitine
Authors: Xu Wei , Grindler Sandra , Kenéz Ákos , Dänicke Sven , Frahm Jana , Huber Korinna 
Published: August 2022

Abstract
Background
Carnitine facilitates the flux of long-chain fatty acids for hepatic mitochondrial beta-oxidation, which acts to ameliorate the negative energy balance commonly affecting high-yielding dairy cows. Inflammation triggered by lipopolysaccharide (LPS) load can however pose a challenge to the metabolic integrity via the expression of pro-inflammatory mediators, leading to immune system activation and respective metabolic alterations. The effect of enhanced carnitine availability on hepatic metabolome profiles during an inflammatory challenge has not yet been determined in dairy cows. Herein, Holstein cows were supplemented with 25 g/d rumen-protected carnitine from 42 d prepartum until 126 d postpartum (n = 16) or assigned to the control group with no supplementation during the same period (n = 14). We biopsied the liver of the cows before (100 d postpartum) and after (112 d postpartum) an intravenous injection of 0.5 µg/kg LPS. Liver samples were subjected to a targeted metabolomics analysis using the AbsoluteIDQ p180 Kit (Biocrates Life Sciences AG, Innsbruck, Austria).

Results
Multivariate statistical analyses revealed that hepatic metabolome profiles changed in relation to both the carnitine supplementation and the LPS challenge. Comparing the metabolite profiles on 100 d, carnitine increased the concentration of short- and long-chain acyl-carnitines, which may be explained by an enhanced mitochondrial fatty acid shuttle and hence greater energy availability. The LPS injection affected hepatic metabolite profiles only in the carnitine supplemented group, particularly altering the concentration of biogenic amines.

Conclusions
Our results point to interactions between an acute hepatic inflammatory response and biogenic amine metabolism, depending on energy availability.
Wearable reproductive trackers: quantifying a key life history event remotely
Authors: Ozsanlav-Harris Luke , Griffin Larry R. , Weegman Mitch D. , Cao Lei , Hilton Geoff M. , Bearhop Stuart 
Published: August 2022

AbstractAdvancements in biologging technology allow terabytes of data to be collected that record the location of individuals but also their direction, speed and acceleration. These multi-stream data sets allow researchers to infer movement and behavioural patterns at high spatiotemporal resolutions and in turn quantify fine-scale changes in state along with likely ecological causes and consequences. The scope offered by such data sets is increasing and there is potential to gain unique insights into a suite of ecological and life history phenomena. We use multi-stream data from global positioning system (GPS) and accelerometer (ACC) devices to quantify breeding events remotely in an Arctic breeding goose. From a training set of known breeders we determine the movement and overall dynamic body acceleration patterns indicative of incubation and use these to classify breeding events in individuals with unknown reproductive status. Given that researchers are often constrained by the amount of biologging data they can collect due to device weights, we carry out a sensitivity analysis. Here we explore the relative merits of GPS vs ACC data and how varying the temporal resolution of the data affects the accuracy of classifying incubation for birds. Classifier accuracy deteriorates as the temporal resolution of GPS and ACC are reduced but the reduction in precision (false positive rate) is larger in comparison to recall (false negative rate). Precision fell to 94.5%, whereas recall didn’t fall below 98% over all sampling schedules tested. Our data set could have been reduced by c.95% while maintaining precision and recall > 98%. The GPS-only classifier generally outperformed the ACC-only classifier across all accuracy metrics but both performed worse than the combined GPS and ACC classifier. GPS and ACC data can be used to reconstruct breeding events remotely, allowing unbiased, 24-h monitoring of individuals. Our resampling-based sensitivity analysis of classifier accuracy has important implications with regards to both device design and sampling schedules for study systems, where device size is constrained. It will allow researchers with similar aims to optimize device battery, memory usage and lifespan to maximise the ability to correctly quantify life history events.
Pharmacovigilance of cutaneous adverse drug reactions in associations with drugs and medical conditions: a retrospective study of hospitalized patients
Authors: Zheng Lei , Jin Hao-bin , Guan Yu-yao , Yang Jing 
Published: August 2022

AbstractBackgroundCutaneous adverse drug reaction (CADR) is a common problem in clinical medication. This study aimed to investigate the correlation between clinical drug application and CADR occurrence as evidence for preventive strategies and rational clinical drug use.MethodsWe analyzed the characteristics of CADRs of 858 patients admitted to Shandong Provincial Third Hospital from March 2007 to December 2018. The most significant drugs concerning the common skin symptoms and their significance to CADR were investigated by case-non-case and multiple logistic regression analyses.ResultsA total of 266 drugs were involved in 858 cases of CADR. Among the ten most relevant medications, primarily antibiotics and herbal injections, and nutritional support drugs, potassium sodium dehydroandrographolide succinate injection, and cefoperazone sodium and sulbactam sodium injection were found to be 2.1 and 1.45 times statistically more prone to CADRs than to other adverse drug reactions (ADRs), respectively. The main route of administration was intravenous (63.16%), with oral administration accounting for 25.19%. There were 747 cases of ADR, 71 of severe ADR, 2 of new and severe ADRs, and 38 cases of new ADR. Overall, 100 cases of CADR exhibited abnormal alanine aminotransferase, aspartate aminotransferase, and serum creatinine levels. The predictive factors for severe CADR occurrence included allergy and smoking histories, cefoperazone sodium, sulbactam sodium injection, levofloxacin lactate and sodium chloride injection.ConclusionsDrug-induced CADR symptoms are commonly associated with other ARDs, predominantly rashes and pruritus, and are often accompanied by some medical conditions, especially liver and kidney damage. Detailed attention to a patient’s primary diseases, allergy history, and drug safety profile could help prevent or reverse CADR in most patients.
HIV, tuberculosis, diabetes mellitus and hypertension admissions and premature mortality among adults in Uganda from 2011 to 2019: is the tide turning?
Authors: Kazibwe Andrew , Bisaso Kuteesa Ronald , Kyazze Andrew Peter , Ninsiima Sandra , Ssekamatte Phillip , Bongomin Felix , Baluku Joseph Baruch , Kibirige Davis , Akabwai George Patrick , Kamya Moses R. , Mayanja-Kizza Harriet , Byakika-Kibwika Pauline , Kagimu Magid , Kalyesubula Robert , Andia-Biraro Irene 
Published: August 2022

Abstract
Background
The growing burden of diabetes mellitus (DM) and hypertension (HTN) on the background of endemic Human Immuno-deficiency Virus (HIV) and tuberculosis (TB) is a concern in low- and middle-income countries. We aimed to describe annual trends in admissions, mortality rates and premature mortality (years of potential life lost—YPLLs) due to HIV, tuberculosis (TB), diabetes mellitus (DM) and hypertension (HTN) in Uganda.

Methods
We conducted a retrospective cohort study, retrieving electronic records of adults admitted to Mulago and Kiruddu national referral hospitals medical wards between 1st January 2011 and 31st December 2019. We used STATA BE 17.0 and GraphPad Prism 8.0.2 to compute total admissions, inpatient crude mortality rates, and YPLLs; and demonstrate trends using Mann–Kendall test.

Results
Of 108,357 admissions, 55,620 (51.3%) were female, 15,300 (14.1%) were recorded in 2012, and 22,997 (21.2%) were aged 21–30 years. HIV, TB, DM and HTN accounted for 26,021 (24.0%); 9537 (8.8%); 13,708 (12.7) and 13,252 (12.2%) of all admissions, respectively. Overall inpatient mortality was 16.7% (18,099/108,357), 53.5% (9674/18,099) were male, 21.5% (3898) were aged 31–40 years and 2597 (14.4%) were registered in 2013. HIV, TB, DM and HTN accounted for 35.6% (6444), 14.6% (2646), 9.1% (1648) and 11.8% (2142) of all deaths, respectively. Total admissions (Kendall’s tau-B = − 0.833, p < 0.001) and deaths declined (Kendall’s tau-B = − 0.611, p = 0.029). A total of 355,514 (mean = 20.8 years, SD 30.0) YPLLs were recorded, of which 54.6% (191,869) were in males; 36.2% (128,755) were among those aged 21–30 years and were recorded in 2012 (54,717; 15.4%). HIV, TB, DM and HTN accounted for 46.5% (165,352); 19.5% (69,347); 4.8% (16,991) and 4.5% (16,167) of YPLLs, respectively. Proportionate contribution of HIV to deaths and YPLLs declined, remained stagnant for TB; and increased for both DM and HTN.

Conclusion
TB and HIV account for higher though declining, while DM and HTN account for lower albeit rising morbidity and premature mortality among adult medical patients in Uganda. TB prevention and treatment; and DM/HTN service integration in HIV care should be optimized and scaled up.
Identification of some native entomopathogenic fungal species and their pathogenicity against Apple Blossom Beetle, Tropinota (Epicometis) hirta (Poda, 1761) (Coleoptera: Cetoniidae) adults
Authors: Uçar Seher , Atay Turgut , Yanar Yusuf 
Published: August 2022

Abstract
Background
Entomopathogenic fungi (EPFs) have more important role in biological control of the important insect pests. In the present study 15 EPF isolates, selected from the EPF culture collection, were identified using molecular methods and their effectiveness against adults of Apple Blossom Beetle (ABB) [Tropinota (= Epicometis) hirta (Poda, 1761) (Coleoptera: Cetoniidae)] was investigated. To determine virulence of isolates against ABB adults, primarily a single-concentration trial was conducted at 1 × 107 conidia ml−1. Furthermore, concentration-mortality trials were conducted with some isolates that were determined to have a high effect, employing concentrations of 1 × 106, 1 × 108, and 1 × 109 conidia ml−1. For isolates tested in concentration-mortality trials, LT30, LT50 and LT90 values at a concentration of 1 × 108 conidia ml−1 were calculated using probit analysis.

Results
Molecular diagnostic tests revealed that the 13 isolates were Beauveria bassiana, 1 isolate was Clonostachys rosea, and 1 isolate was Purpureocillium lilacinum. According to the single- concentration trial data, some isolates with high efficacy were selected (GN16, GN22-1, GN29-1, HP3-1 and HP33-1) and concentration-mortality trials were established with these isolates using 1 × 106, 1 × 108 and 1 × 109 conidia ml−1 concentrations. According to the results of the concentration-mortality trials, all isolates used in 1 × 108 and 1 × 109 conidia ml−1 concentrations started to show a significant effect from the 11th day, and at the end of the 17th day, the effect in all concentrations was over 90% (except 1 × 106 conidia ml−1 concentration of GN22-1).

Conclusions
According to the data obtained, all isolates used in concentration-mortality trials were effective against ABB adults. With these isolates, more detailed studies should be conducted under orchard conditions.
Comparison of psychiatric disorders in addicted patients treated with buprenorphine, methadone, and opium tincture in Iran
Authors: Bahadori Zahra , Safaie Nilufar , Mirmohammadkhani Majid 
Published: August 2022

Abstract
Background
The aim of this study was to comparing the complications (depression, anxiety, sleep disorders and sexual dysfunction) of buprenorphine, methadone and opium tincture as an alternative drug treatment in patients referred to the National Center for Addiction Studies of Tehran during 2020–2021. This cross-sectional study was concocted on 197 patients referred to the National Center for Addiction Studies of Tehran who were treated with one of the alternative treatments of opioids buprenorphine (n = 24), methadone (n = 116), and opium tincture (n = 57) during 2020–2021. Three questionnaires were used to assess the side effects of drugs including Anxiety and Depression Hospital Scales (HADS), AIS (Athens insomnia scale) and the International Index of Erectile Function (IIEF). Data were analyzed using Stata 14.0. The multinomial logistic regression model was used in order to compare complications in the three groups and adjusted odds ratio (OR) with 95% confidence interval (CI) were estimated.

Results
The mean age in the buprenorphine, methadone and opium tincture groups were 46.80 ± 9.90, 48.31 ± 13.33 and 55.30 ± 10.34 years, respectively. The numbers of men were 17 (70.80), 90 (77.60), and 50 (78.70); respectively. Multinomial logistic regression model showed OR = 0.73 (95% CI 0.61–0.88) for anxiety in the methadone group in compared to buprenorphine. Likewise, OR = 1.22 (95% CI 1.001–1.48) and OR = 1.34 (95% CI 1.10–1.63) was observed for sleep disorder in the methadone and opium tincture groups in compared to buprenorphine; respectively.

Conclusions
Sleep disturbance in buprenorphine group is less than opium tincture and methadone. Also, anxiety in the methadone group was lower than the buprenorphine, however, there was no significant difference between the three groups in term of sexual function and depression. In addition to control measures for sleep disorders in receiving methadone and buprenorphine, the cohort studies with a higher sample size are recommended.