bluuebunny/crossref_metadata_2025_split huggingface dataset:
Atrial myocytes-derived exosomal microRNA contributes to atrial fibrosis in atrial fibrillation
Authors: Hao Hongting , Yan Sen , Zhao Xinbo , Han Xuejie , Fang Ning , Zhang Yun , Dai Chenguang , Li Wenpeng , Yu Hui , Gao Yunlong , Wang Dingyu , Gao Qiang , Duan Yu , Yuan Yue , Li Yue 
Published: June 2022

Abstract
Background Atrial fibrosis plays a critical role in the development of atrial fibrillation (AF). Exosome is a promising cell-free therapeutic approach for the treatment of AF. The purpose of this study was to explore the mechanisms underlying exosomes derived from atrial myocytes regulated atrial remodeling and ask whether their manipulation allows for therapeutic modulation of fibrosis potential abnormalities during AF. MethodsWe isolated exosomes from atrial myocytes and patients serum, microRNA (miRNA) sequencing analyzed the exosomal miRNAs in atrial myocytes-exosomes and patients serum-exosomes. mRNA sequencing and bioinformatics analysis corroborate the key gene as direct targets of miR-210-3p.ResultsThe miRNAs sequencing analysis identified that miR-210-3p expression significantly increased in exosomes of tachypacing atrial myocytes and serum of AF patients. In vitro, the analysis showed that miR-210-3p inhibitor reversed tachypacing-induced proliferation and collagen synthesis in atrial fibroblasts. Accordingly, KO miR-210-3p could reduce the incidence of AF and ameliorate atrial fibrosis induced by Ang Ⅱ. The mRNA sequencing analysis and Dual-Luciferase reporter assay proved that glycerol-3-phosphate dehydrogenase 1-like (GPD1L) is the potential target gene of miR-210-3p. The functional analysis suggests that GPD1L regulated atrial fibrosis via PI3K/AKT signaling pathway. Besides, silencing GPD1L in atrial fibroblasts induced cells proliferation and these effects could be reversed by PI3K inhibitor (LY294002). Conclusion We demonstrate that atrial myocytes-derived exosomal miR-210-3p promoted the proliferation and collagen synthesis via inhibiting GPD1L in atrial fibroblasts. Preventing pathological crosstalk between atrial myocytes and fibroblasts may as a novel target to improve atrial fibrosis in AF.
AHSA1 promotes proliferation and EMT by regulating ERK/CALD1 axis in hepatocellular carcinoma
Authors: Zhang Jiakang , Ren Zhixuan , Song Zhenghui , Lin Junhao , Luo Yue , Zou Xiaopei , Pan Yingying , Qi Na , Li Aimin , Liu Xinhui 
Published: June 2022

Abstract
Background
Hepatocellular carcinoma (HCC) is one of the major causes of cancer-related death worldwide. AHSA1 as a chaperone of HSP90 promotes the maturation, stability, and degradation of related cancer-promoting proteins. However, the regulatory mechanism and biological function of AHSA1 in HCC are largely unknown. The purpose of this study is to elaborate the role of AHSA1 in HCC development.
Methods
Real-Time PCR, Western blotting, and Immunohistochemistry were used to determine the mRNA and protein expression of AHSA1 in liver epithelial cells, HCC cells, and tissues. Furthermore, both in vitro and in vivo, gain- and loss-of-function studies demonstrated the role of AHSA1 in the proliferation, invasion, metastasis, and epithelial-mesenchymal transition of HCC. To explore the molecular mechanism of AHSA1 in HCC, Western blotting, coimmunoprecipitation assay, and mass spectrometer assay were performed.
Results
Actually, we found AHSA1 was significantly upregulated in HCC tissues and cell lines, and was correlated with the poor prognosis of HCC patients. Furthermore, both in vitro and in vivo, gain- and loss-of-function studies demonstrated AHSA1 promoted the proliferation, invasion, metastasis, and epithelial-mesenchymal transition (EMT) of HCC. Moreover, the mechanistic study indicated that AHSA1 recruited ERK1/2 and promoted the phosphorylation and inactivation of CALD1, while ERK1/2 phosphorylation inhibitor, SCH772984, reversed the role of AHSA1 in the proliferation and EMT of HCC. Furthermore, we demonstrated the knockdown of CALD1 reversed the inhibition of proliferation and EMT by knocking AHSA1 in HCC. We also illustrated a new molecular mechanism associated with AHSA1 in HCC that was independent of HSP90 and MEK1/2.
Conclusions
In summary, AHSA1 may play an oncogenic role in HCC by regulating ERK/CALD1 axis and serve as a novel therapeutic target for HCC.
Neuroprotective action of α-Klotho against LPS- activated glia conditioned medium in primary neuronal culture
Authors: Nakao Vinicius Wanatable , Mazucanti Caio Henrique Yokowama , Lima Larissa Sá , Mello Paloma Segura , Souza Natacha Medeiros , Kinoshita Paula Fernanda , Leite Jacqueline Alves , Kawamoto Elisa Mitiko , Scavone Cristoforo 
Published: June 2022

Abstract
The α-Klotho is an anti-aging protein that when overexpressed extends the life span in humans and mice. It has an anti-inflammatory and protective action on renal cells by inhibiting NF-κB activation and production of inflammatory cytokines in response to TNF-α. Furthermore, studies have shown the neuroprotective effect of α-Klotho against neuroinflammation on different conditions, such as aging, animal models of neurodegenerative diseases, and ischemic brain injury. This work aimed to evaluate the effects of α-Klotho protein on primary glial cell culture against the proinflammatory challenge with LPS and how this could interfere in neuronal health. Cortical mixed glial cells and purified astrocytes were pretreated with α- α-Klotho and stimulated with LPS followed by TNFα, IL-1β, IL-6, IFN-γ levels and NF-κB activity analysis. Conditioned medium from cortical mixed glia culture treated with LPS (glia conditioned medium (GCM) was used to induce neuronal death of primary cortical neuronal culture and evaluate if GCM-KL (GCM-KL: medium from glia culture pretreated with α- α-Klotho followed by LPS stimulation) can reverse this effect. LPS treatment in glial cells induced an increase in proinflammatory mediators such as TNF-α, IL-1β, IL-6, and IFN-γ, and activation of astrocyte NF-κB. GCM treated-cortical neuronal culture induced a concentration-dependent neuronal death. Pretreatment with α-Klotho decreased TNF-α and IL-6 production, revert NF-κB activation and blocked neuronal death induced by GCM. These data suggest an anti-inflammatory and neuroprotective effect of α-Klotho protein in the CNS. This work demonstrated the therapeutic potential of α-Klotho in pathological processes which involves a neuroinflammatory component.
A green attapulgite bacterial cellulose imprinted aerogel for selective adsorption and recovery of Dy(III)
Authors: Zheng Xudong , Xu Tongtong , Sun Wen , Mei Jinfeng , Zhou Man , Li Zhongyu 
Published: June 2022

Abstract
Recycling dysprosium from the environment can not only alleviate the industrial crisis, but also reduce the ecological pollution caused by dysprosium ions. At present, the price of the materials used to adsorb rare earth ions is generally high, which is not conducive to the wide application of the adsorbents in the rare earth industry. In this paper, based on the 3D network structure of carboxylated bacterial cellulose, some cheap materials such as attapulgite and gelatin were used to replace the high-priced raw materials, and a low-cost and green I-APT-GT-OBC aerogel was prepared. To test the adsorption properties of the composite aerogel, we carried out a series of adsorption experiments. The results showed that the saturated adsorption capacity of dysprosium ions by the imprinted aerogel was 48.762 mg g− 1 when the pH value was 5.0, and the adsorption process was spontaneous, endothermic and entropy increasing. In the competitive adsorption experiment, the Kd value of I-APT-GT-OBC for dysprosium ions was 4084.40 mL g− 1, which was much higher than that of other aerogels, showing excellent selective adsorption. In conclusion, I-APT-GT-OBC is expected to be a low-cost adsorbent that can efficiently adsorb dysprosium ions in water.
Chromosome-level genome of Capitulum mitella reveals an ancient whole-genome duplication event and intertidal adaptation of barnacles
Authors: Yuan Jianbo , Zhang Xiaojun , Zhang Xiaoxi , Sun Yamin , Liu Chengzhang , Li Shihao , Yu Yang , Zhang Chengsong , Jin Songjun , Wang Min , Xiang Jianhai , Li Fuhua 
Published: June 2022

Abstract
Barnacles are the only sessile crustaceans inhabiting intertidal zone, an extremely stressful environment for sessile organisms. Herein, we report the chromosome-level genome of a stalked barnacle, Capitulum mitella, which is a dominant intertidal cirripede of the west Pacific Ocean coast. After comprehensive comparative genomic analyses, it is first time to find an ancient whole-genome duplication (WGD) event that preceded the divergence of Lepadomorpha and Sessilia approximately 237 million years ago. The retained duplicated genes of WGD are primarily enriched in many environmental information processing pathways, shedding lights on its adaptive evolution of intertidal sessile life. In addition, transcriptomic and metabolomic sequencing and analyses of C. mitella indicated that the upregulation of some expanded anti-stress factors and accumulation of acyl-carnitines help barnacles adapt to stressful intertidal conditions. Therefore, this study provides a valuable resource for understanding the unique intertidal adaptation mechanism of sessile crustaceans, and reveals novel WGD events in invertebrates.
Mapping genetic determinants for grain physicochemical and nutritional traits in unpolished rice using genome-wide association analysis
Authors: Chattopadhyay Krishnendu , C Anilkimar , Bagchi Torit Baran , Sanghamitra Priyadarshini , Sarkar Sutapa , Marndi Bishnu Charan , Kumar Awadesh , Moharana Nutan , Mohapatra Shuvendu Shekhar , Sahoo Soumay Kumar 
Published: June 2022

Abstract
Public awareness is gradually growing in favour of consuming nutritionally superior brown and pigmented rice. But unpolished rice is relatively poor in eating and cooking quality. Understanding inheritance pattern of nutritional and quality traits is prerequisite for further improvement exercising molecular tools. A holistic approach was adopted to identify unique and common genomic regions regulating 17 grain nutritional as well as physiochemical traits using a diverse panel of 96 rice genotypes. Seventy eight significant marker-trait association distributed in all chromosomes with PVE ranging from 4–27% were detected. Marker RM 467 was co-localized with previously identified QTL qPC10.1 and gene OsGluA2. Grain protein and metal content were associated with cooking quality which was also supported by the marker-trait association. Two QTLs for each of grain protein and amylose content with associated markers RM 17600 and RM 1272 were found co-localized with additive effect in opposite direction. Similarly, RM 162 was associated with both zinc content and gelatinization temperature (alkali spreading value). Iron content was also associated with grain size which was supported by the association of RM8050 with the both Fe content and kernel length/breadth ratio. Phenotypically pigmented rice was detected with low amylose content and one genetic loci for anthocyanin (qANTH5.1) was also found to be co-localized with a QTL for AC (qAC5.1) with additive effect in opposite direction. Co-localized associated loci for nutritional and cooking and eating quality can guide strategic biofotification programs for improving rice for nutritional traits without distracting consumer preference.
Whether the infracardiac bursa protect right pleura during laparoscopic radical operation of Siewert type II adenocarcinoma of esophagogastric junction?
Authors: Lin Zeyu , Zeng Haiping , Xiong Wenjun , Li Jin , Chen Yan , Luo Lijie , Zheng Yansheng , Zhang Zhuoxuan , Wang Wei 
Published: June 2022

Abstract
Background
Transthoracic single-port assisted laparoscopic five-step maneuver inferior mediastinal lymphadenectomy for Siewert type II adenocarcinoma of esophagogastric junction (AEG) has superiority in lower mediastinal lymph nodes dissection and digestive tract reconstruction. However, the right pleura was probably ruptured in this surgical technique. The aim of this study was to explore whether the infracardiac bursa (ICB) exposed could protect right pleura.
Methods
We retrospectively collected and evaluated the clinical and pathological data of patients who underwent five-step maneuver of transthoracic single-port assisted laparoscopic lower mediastinal lymphadenectomy for Siewert II AEG at Guangdong Provincial Hospital of Chinese Medicine between May 2017 and February 2022.
Results
A total of 49 patients were eligible, including 31 patients in ICB exposed group (group A) and 18 patients in ICB unexposed group (group B). There were no statistically significant differences in baseline characteristics between the two groups. 4 patients (12.9%) had right pleura rupture in group A, while 14 patients (77.8%) in group B, and the difference was statistically significant (p < 0.001). Compared with group B, the extubation time of endotracheal intubation (10.0 (6.0 ~ 12.0) vs. 13.0 (8.0 ~ 15.0) min, p = 0.003) and thoracic drainage tube stay (6.0 (5.0 ~ 7.0) vs. 8.0 (6.0 ~ 10.5) days, p = 0.041) were significantly shorted in the group A. The drainage volume of thorax (351.61 ± 125.00 vs. 418.61 ± 207.86 mL, p = 0.146) was non-significant less and the rate of complications (3.2% vs. 11.1%, p = 0.074) was non-significant lower in group A compared with group B. The postoperative hospital stay (9.0 (8.0,13.0) vs. 9.0 (8.0,12.0) days, p = 0.983) were similar in two groups. No serious adverse event occurred in any patient.
Conclusions
The ICB exposed could protect the right pleura and may promote postoperative recovery, which may be used as an anatomical marker in inferior mediastinal lymphadenectomy.
The relationship of coagulation and inflammation indicators with different severity gastrointestinal bleeding in adult with Henoch–Schonlein purpura
Authors: Zhang Ya , Yang Hong , Yang Weiwen , Liu Qi 
Published: June 2022

Abstract
According to shock index, adult patients with HSP-related different severity gastrointestinal bleeding of this study were classified into different severity groups, aiming at analyzing and preliminarily discussing the clinical vaule of the above common blood indicators in such kinds of patient. Adult patients with HSP-related different severity gastrointestinal bleeding were retrospectively selected. Meanwhile, the healthy subjects were included as the healthy control group. According to the shock index grouping criteria, those patients were divided into three groups: no shock group, mild shock group and moderate shock group. The general data, laboratory indicators of all patients and healthy subjects were recorded in detail, and calculating NLR,PLR, then comparing the differences in the above common indicators among groups by statistical analysis. Comparing to healthy control group, WBC, N, PLR and NLR in no shock, mild shock and moderate shock groups were higher (P＜0.05); the WBC, N, hs-CRP, NLR and PLR in moderate shock group were higher than no shock and mild shock group(P＜0.05). Comparing to healthy control group, PLT, FIB and D-D in no shock, mild shock and moderate shock groups were higher (P＜0.05), and PT, INR in moderate shock group were prolonged while MPV decreased (P＜0.05); comparing to no shock group, FIB and D-D in moderate shock group were higher, while MPV decreased(P＜0.05); D-D in moderate shock group was higher than mild shock group(P＜0.05);difference in immunization and complement indicators among no shock, mild shock and moderate shock groups had no statistical significance(P＞0.05); PLR in moderate shock group was positively correlated with shock index(P＜0.05), r=0.662.There are differences on common coagulation indicators in adult patients with HSP-related different severity gastrointestinal bleeding, especially D-D is higher in moderate shock group than any other group;WBC, N, hs-CRP, PLR and NLR are different among different groups, and PLR in moderate shock group is positively correlated with shock index, higher PLR indicating more severe disease, so the above clinical indicators can be used to assess the severity of adult patients with HSP-related different severity gastrointestinal bleeding.
Influence of Two Kinds of Clearance joints on the Dynamics of Mechanical System Based on Modified Contact Force
Authors: Tan Haiyan , Li Li , Huang Qiang , Jiang Zhuoda , Li Qingxiang 
Published: June 2022

Abstract
This study takes the slider-crank mechanism with revolute joint and translational joint as the research object and studies the contact force model of the clearance joint and the influence of the hybrid clearance joints on the nonlinear dynamic behavior of the mechanism. A modified contact force model is established based on the simplified elastic oscillator model (as a normal force in clearance joint), which can support the calculation of contact force for both fully elastic recovery, non-elastic recovery and fully inelastic recovery. Based on the LuGre friction model, the tangential friction model of the clearance joint is given. Thus, the normal force and tangential force during the dynamic contact of the clearance joint are formed. Combining Lagrange’s equations of the first kind with the modified normal force and tangential friction force, the dynamic equations of the multi-body system with clearance joints are established. The Baumgarte stabilization method is used to improve the numerical stability. The correctness of the dynamic prediction model in the mechanism with clearance joint is verified by experiment. The dynamic analysis of the slider-crank mechanism with mixed clearance joints shows that the revolute clearance joint has a greater influence on the mechanism than the translational clearance, and the revolute clearance joint plays a leading role in the dynamic response.
Investigating the causal inference of vaccine on COVID-19 disease propagation based on Bayesian approach
Authors: Ling Lu , Ukkusuri Satish V. 
Published: June 2022

Abstract
The causal impact of COVID-19 vaccine coverage on effective reproduction number R(t) under the disease control measures in the real-world scenario is understudied, making the optimal reopening strategy (e.g., when and which control measures are supposed to be conducted) during the recovery phase difficult to design. In this study, we examine the demographic heterogeneity and time variation of the vaccine effect on disease propagation based on the Bayesian structural time series analysis. Furthermore, we explore the role of non-pharmaceutical interventions (NPIs) and the entrance of the Delta variant of COVID-19 in the vaccine effect for U.S. counties. The analysis highlights several important findings: First, vaccine effects vary among the age-specific population and population densities. The vaccine effect for areas with high population density or core airport hubs is 2 times higher than for areas with low population density. Besides, areas with more older people need a high vaccine coverage to help them against the more contagious variants (e.g., the Delta variant). Second, the business restriction policy and mask requirement are more effective in preventing COVID-19 infections than other NPI measures (e.g., bar closure, gather ban, and restaurant restrictions) for areas with high population density and core airport hubs. Furthermore, the mask requirement consistently amplifies the vaccine effects against disease propagation after the presence of contagious variants. Third, areas with a high percentage of older people are suggested to postpone relaxing the restaurant restriction or gather ban since they amplify the vaccine effect against disease infections. Such empirical insights assist recovery phases of the pandemic in designing more efficient reopening strategies, vaccine prioritization, and allocation policies.
Converting N2 molecules into NH3 with TiO2/Fe3O4 composite covered with a thin water layer under ambient condition
Authors: Moriya Ichiro 
Published: June 2022

Abstract
As ammonia manufacture today require numerous energy and very pure hydrogen gas and moreover emit large quantities of CO2, researches for new ammonia synthesis methods are actively performed.Here, author reports the novel method through which N2 molecules in air is reduced into ammonia with TiO2/Fe3O4 composite having thin water layer on composite’s surface under ambient condition ( approximately 50℃ and atmospheric pressure). The composites were composed of both nm-sized TiO2 particles and μm-sized Fe3O4 ones. First, composites were held in refrigerator, mainly at that time, N2 molecules in air adsorbed onto surface of composite. Next, the composite was irradiated with various lights including solar light, 365nm LED light and tungsten light through thin water layer formed by condensation of water vapour in air.Reliable amount of ammonia was obtained under 5 minute’s irradiation of solar light or of both 365m LED light and 500W tungsten light. Interface of TiO2 particles and Fe3O4 ones played precious role. This reaction was catalytic reaction promoted by photocatalytic one. Before irradiation of light, holding in freezer instead of refrigerator provided larger amount of ammonia. Maximum ammonia yield was approximately 18.7 μmol/g･5min.under irradiation of 300W tungsten light only.
Sonar Data Classification using Neural Network Trained by Hybrid Dragonfly and Chimp Optimization Algorithms
Authors: Mousavipour F. , Mosavi Mohammad Reza 
Published: June 2022

Abstract
This paper proposes a hybrid Dragonfly Algorithm (DA) for training Multi-Layer Perceptron Neural Network (MLP NN) to design the classifier for solving complicated problems and distinguishing the real target from liars’ targets in sonar applications. Due to improving the cost computation and reducing the waste of time, a modified low-cost DA is designed for evaluation. To assess the accuracy of the technique, some well-known meta-heuristic trainers include Particle Swarm Optimization (PSO), Gravitational Search Algorithm (GSA), DA, and Chimp Optimization Algorithm (ChoA) compared to show the accuracy of similar algorithms. DA and ChoA algorithms have remarkable features and a hybrid algorithm of them is proposed. The performance of the proposed classifier will be evaluated by two standard benchmark datasets. The results show that the modified hybrid DA-ChoA has 15% less time consuming and 4% better performance rather than the original dragonfly method.