bluuebunny/crossref_metadata_2025_split huggingface dataset:
CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis
Authors: Sugiyama Shusei , Yamada Kohdai , Denda Miwako , Yamanaka Satoshi , Ozawa Satoshi , Morishita Ryo , Sawasaki Tatsuya 
Published: June 2022

AbstractProtein–protein interaction (PPI) analysis is a key process to understand protein functions. Recently, we constructed a human protein array (20 K human protein beads array) consisting of 19,712 recombinant human proteins produced by a wheat cell-free protein production system. Here, we developed a cell-free protein array technology for proximity biotinylation-based PPI identification (CF-PPiD). The proximity biotinylation enzyme AirID-fused TP53 and -IκBα proteins each biotinylated specific interacting proteins on a 1536-well magnetic plate. In addition, AirID-fused cereblon was shown to have drug-inducible PPIs using CF-PPiD. Using the human protein beads array with AirID-IκBα, 132 proteins were biotinylated, and then selected clones showed these biological interactions in cells. Although ZBTB9 was not immunoprecipitated, it was highly biotinylated by AirID-IκBα, suggesting that this system detected weak interactions. These results indicated that CF-PPiD is useful for the biochemical identification of directly interacting proteins.
Chicken-derived RSPO1 and WNT3 contribute to maintaining longevity of chicken intestinal organoid cultures
Authors: Oost Miriam J. , Ijaz Adil , van Haarlem Daphne A. , van Summeren Kitty , Velkers Francisca C. , Kraneveld Aletta D. , Venema Koen , Jansen Christine A. , Pieters Raymond H. H. , ten Klooster Jean Paul 
Published: June 2022

AbstractIntestinal organoids are advanced cellular models, which are widely used in mammalian studies to mimic and study in vivo intestinal function and host–pathogen interactions. Growth factors WNT3 and RSPO1 are crucial for the growth of intestinal organoids. Chicken intestinal organoids are currently cultured with mammalian Wnt3a and Rspo1, however, maintaining their longevity has shown to be challenging. Based on the limited homology between mammalian and avian RSPO1, we expect that chicken-derived factors are required for the organoid cultures. Isolated crypts from embryonic tissue of laying hens were growing in the presence of chicken WNT3 and RSPO1, whereas growth in the presence of mammalian Wnt3a and Rspo1 was limited. Moreover, the growth was increased by using Prostaglandin E2 (PGE2) and a Forkhead box O1-inhibitor (FOXO1-inhibitor), allowing to culture these organoids for 15 passages. Furthermore, stem cells maintained their ability to differentiate into goblets, enterocytes and enteroendocrine cells in 2D structures. Overall, we show that chicken intestinal organoids can be cultured for multiple passages using chicken-derived WNT3 and RSPO1, PGE2, and FOXO1-inhibitor.
Hematologic and plasma biochemical prognostic indicators for stranded free-ranging phocids presented for rehabilitation
Authors: Vail Matthew , Beaufrère Hugues , Gallini Stefan , Paluch Hubert , Brandão João , DiGeronimo Peter M. 
Published: June 2022

AbstractThis retrospective study used data obtained from medical records of 530 stranded free-ranging harbor (Phoca vitulina), grey (Halichoerus grypus), harp (Pagophilus groenlandicus), and hooded seals (Cystophora cristata) presented to the Marine Mammal Stranding Center in Brigantine, New Jersey from January 1998 through December 2016. The objective was to identify hematological and plasma biochemical parameters of seals at time of presentation that were associated with successful rehabilitation and with duration of hospitalization using univariate and multivariate logistic regressions. At presentation, animals that subsequently survived rehabilitation had greater alkaline phosphatase activity and absolute lymphocyte and total calcium concentrations and lower blood urea nitrogen, sodium, chloride, phosphorus, and total bilirubin concentrations and lower aspartate aminotransferase and alanine aminotransferase activities than animals that eventually died or were euthanized while under care. Results suggest that young, actively growing animals are more likely to survive rehabilitation and that bloodwork consistent with dehydration, systemic disease, and exhaustion are negative prognostic indicators. These results provide prognostic indicators that may aid clinical decision-making for seals presented for rehabilitation.
PEG2000-DBCO surface coating increases intracellular uptake of liposomes by breast cancer xenografts
Authors: Liu Daxing , Cohen Jules , Turkman Nashaat 
Published: June 2022

AbstractGiven our interest in the utility of liposomes for molecular imaging and theranostics, we investigated how coating the outer layer of the liposome affects internalization by breast cancer cell lines in vitro and in breast tumor tissues in vivo. Indeed, we discovered that a remarkably high liposomal uptake can be achieved by DBCO (dibenzocyclooctyne) soft coating. Our data demonstrates that decorating the terminal lipid with a DBCO moiety at a specific density induces increased tumor uptake in vivo (tumor uptake ~ 50%) compared to conventional undecorated liposome (tumor uptake ~ 20%). In this study, we report improved visualization of breast cancer cells in vivo using a 4T1 orthotopic breast cancer model and primary breast tumor xenograft models MDA-MB-231 and MDA-MB-436. L-PEG2000-DBCO coated liposomes demonstrate increased accumulation in breast cancer cells independent of tumor size, type, position, receptor expression, as well as the condition of the host mice. We expect these findings to have a major positive impact on the practical utility of liposomes in image-guided applications and precision medicine theranostics.
Serum anti-malondialdehyde-acetaldehyde IgA antibody concentration improves prediction of coronary atherosclerosis beyond traditional risk factors in patients with rheumatoid arthritis
Authors: Lomzenski Hannah E. , Thiele Geoffrey M. , Duryee Michael J. , Chen Sheau-Chiann , Ye Fei , Anderson Daniel R. , Mikuls Ted R. , Ormseth Michelle J. 
Published: June 2022

AbstractPatients with rheumatoid arthritis (RA) have increased atherosclerosis; oxidative stress may be a contributor. Oxidative stress produces immunogenic malondialdehyde-acetaldehyde (MAA) protein adducts and anti-MAA antibodies are detectable in human serum. We hypothesized that anti-MAA antibody concentrations are associated with coronary atherosclerosis in RA patients. Serum concentrations of anti-MAA antibodies (IgA, IgG, and IgM) were measured in 166 RA patients using ELISA cross-sectionally. Relationship between anti-MAA antibody concentrations and cardiovascular and metabolic measures and predictive accuracy of anti-MAA antibodies for presence of coronary artery calcium (CAC) and high CAC (≥ 300 Agatston units or ≥ 75th percentile) were assessed. Only serum IgA anti-MAA antibody concentration was associated with increased CAC, insulin resistance, and decreased high-density lipoprotein particle number. When added as an interaction term with ACC/AHA 10-year risk score plus high-sensitivity C-reactive protein, IgA anti-MAA antibody concentration improved the C-statistic for prediction of any CAC and high CAC compared to ACC/AHA 10-year risk score plus hs-CRP alone. IgA anti-MAA concentration is associated with multiple cardiovascular risk factors and modifies the relationship between ACC/AHA 10-year risk score and CAC in RA patients. IgA anti-MAA concentration could assist in prediction of atherosclerotic CVD and risk stratification when added to standard measures of cardiovascular risk.
A phylogenetic approach to study the evolution of somatic mutational processes in cancer
Authors: Miura Sayaka , Vu Tracy , Choi Jiyeong , Townsend Jeffrey P. , Karim Sajjad , Kumar Sudhir 
Published: June 2022

AbstractCancer cell genomes change continuously due to mutations, and mutational processes change over time in patients, leaving dynamic signatures in the accumulated genomic variation in tumors. Many computational methods detect the relative activities of known mutation signatures. However, these methods may produce erroneous signatures when applied to individual branches in cancer cell phylogenies. Here, we show that the inference of branch-specific mutational signatures can be improved through a joint analysis of the collections of mutations mapped on proximal branches of the cancer cell phylogeny. This approach reduces the false-positive discovery rate of branch-specific signatures and can sometimes detect faint signatures. An analysis of empirical data from 61 lung cancer patients supports trends based on computer-simulated datasets for which the correct signatures are known. In lung cancer somatic variation, we detect a decreasing trend of smoking-related mutational processes over time and an increasing influence of APOBEC mutational processes as the tumor evolution progresses. These analyses also reveal patterns of conservation and divergence of mutational processes in cell lineages within patients.
Profiles of Swallowing Impairment in a Cohort of Patients With Reduced Tongue Strength Within 3 Months of Cerebral Ischemic Stroke
Authors: Smaoui Sana , Peladeau-Pigeon Melanie , Mancopes Renata , Sutton Danielle , Richardson Denyse , Steele Catriona M. 
Published: June 2022

Purpose:Patients with poststroke dysphagia may experience inefficient bolus clearance or inadequate airway protection. Following a stroke, impairments in lingual pressure generation capacity are thought to contribute to oropharyngeal dysphagia. The goal of our study was to determine whether similar profiles of swallowing impairment would be seen across a cohort of patients with reduced tongue strength within 3 months after cerebral ischemic stroke.Method:The sample comprised six adults with reduced tongue strength (i.e., maximum anterior isometric pressure < 40 kPa). Participants underwent a videofluoroscopy according to a standard protocol. Post hoc blinded ratings were completed using the Analysis of Swallowing Physiology: Events, Kinematics and Timing Method and coded as “typical” versus “atypical” (i.e., within vs. outside the healthy interquartile range) in comparison to published reference values.Results:The videofluoroscopies suggested that having reduced tongue strength did not translate into a common profile. Of the six participants, two showed Penetration–Aspiration Scale (PAS) scores of ≥ 3 on thin liquids, associated with incomplete laryngeal vestibule closure (LVC). Another two participants displayed PAS scores of 2 (transient penetration), but these were not associated with incomplete LVC. Pharyngeal residue, above the healthy 75th percentile, was seen for three participants. Five participants presented with atypical reductions in hyoid XY peak position.Conclusions:In this cohort of adults within 3 months of cerebral ischemic stroke, reductions in tongue strength presented alongside a variety of changes in swallowing physiology. There was no straightforward relationship linking reduced tongue strength to particular patterns of impairment on videofluoroscopy.
Small‐disturbance stability of a concentrating solar power collecting system with multiple synchronous generators
Authors: Du Wenjuan , Ma Zhicheng , Lai Linchen , Liu Jun , Fu Qiang , Wang Haifeng 
Published: June 2022

AbstractThis paper examines the small‐disturbance stability in planning a concentrating solar power collecting system (CSPCS) with multiple concentrating solar power (CSP) generation plants, which are modelled as synchronous generators (SGs). The examination indicates that an oscillation mode of the CSPCS gradually moves to the right half of complex plane when the planned number of the CSP generation plants increases. Such oscillatory instability is different with the low‐frequency electromechanical power oscillations (LEPOs) in a conventional multi‐machine power system, because the unstable oscillation mode is associated with the excitation systems, rather than the electromechanical dynamics of the SGs. Analysis is carried out in the paper to explain why the instability risk found in the planning is associated with the excitation systems instead of electromechanical dynamics of the SGs. The analysis helps to understand the finding made in the planning. Furthermore, rules are proposed to adjust the gain of feedback loop of the excitation systems to eliminate the risk of oscillatory instability. This suggests that the parameters’ setting of excitation systems recommended by the IEEE standard may need to be modified to accommodate the planning of CSPCS. Tests are presented to demonstrate and evaluate the analysis and conclusions made.
Stressbezogene Einflüsse körperlicher Beschwerden für den Schweregrad einer Depression bei jungen Mädchen
Authors: Laessle Reinhold , Schmidt Ulrike 
Published: June 2022

ZUSAMMENFASSUNGAusgehend von bisherigen empirischen Befunden und theoretischen Vorstellungen zur Entstehung und Aufrechterhaltung von Depressionen bei Adoleszenten wurde in der vorliegenden Studie geprüft, inwieweit sich junge Mädchen mit einer Depression bezüglich stressbezogener Faktoren auf psychologischer und körperlicher Ebene von einer gesunden Kontrollgruppe unterscheiden. Es wurden 148 Mädchen mit einem mittleren Alter von 15 Jahren untersucht. Der Ausprägungsgrad der depressiven Symptome wurde zu zwei Messzeitpunkten, zwischen denen ein Zeitintervall von 6 Monaten lag, gemessen. 74 erfüllten zum ersten Messzeitpunkt die DSM-IV-Kriterien für eine Major-Depression. Die Stressbelastung und die Stressverarbeitung sowie körperliche Beschwerden wurden mit dem SSKJ 3–8 und dem SVFKJ sowie dem GBB gemessen. Die Ergebnisse zeigten, dass die depressiven Mädchen eine höhere Stressbelastung auf psychologischer und auf körperlicher Ebene aufweisen und darüber hinaus sehr viel empfindlicher gegenüber Stress waren. Dem gegenüber standen die Anwendung destruktiver Strategien zur Stressverarbeitung wie zum Beispiel Aggressivität und der fehlende Gebrauch konstruktiver Verhaltensweisen wie zum Beispiel Problemlösen oder die Suche nach sozialer Unterstützung. Körperliche Beschwerden wie Gliederschmerzen und Kreislaufprobleme hatten einen signifikanten Einfluss auf den Schweregrad der Depression 6 Monate später. Die Ergebnisse können gut in das kognitive Modell von Beck eingeordnet werden.
Moderne perioperative Versorgungskonzepte in der Thoraxchirurgie:
Enhanced Recovery After Thoracic Surgery (ERATS)
Authors: Piler Tomas , Creutzenberg Marcus , Hofmann Hans-Stefan , Ried Michael 
Published: June 2022

ZusammenfassungBei den modernen perioperativen Versorgungskonzepten ermöglicht das multimodale ERAS (Enhanced Recovery After Surgery) eine Verbesserung der postoperativen Rekonvaleszenz von chirurgischen
Patienten nach einem operativen Eingriff. Es wird durch die sog. ERAS-Society geleitet und Kliniken können sich offiziell zertifizieren lassen. Der Schwerpunkt des ERAS-Konzepts liegt in einer
einheitlichen Betreuung des Patienten bereits vor der Aufnahme bis zur Entlassung mit dem Ziel einer Verbesserung der perioperativen Abläufe durch Implementierung von evidenzbasierten
Protokollen unter Beteiligung eines multidisziplinären Behandlungsteams. Im Jahr 2019 erschienen erstmalig ERAS-Guidelines der europäischen Gesellschaft für Thoraxchirurgie (ESTS = European
Society of Thoracic Surgery) in Kooperation mit der ERAS-Society speziell für lungenresezierende Eingriffe, welche insgesamt 45 Empfehlungen bzw. Enhanced Recovery Pathways (ERP)
unterschiedlicher Graduierung definierten. Durch die Implementierung von ERAS-Konzepten in der Thoraxchirurgie (ERATS = Enhanced Recovery After Thoracic Surgery) sollen standardisierte
perioperative Abläufe basierend auf Studienergebnissen und/oder Expertenempfehlungen etabliert werden. Diese Empfehlungen berücksichtigen neben organisatorischen Aspekten vor allem
thoraxchirurgische und anästhesiologische Vorgehensweisen mit dem übergeordneten Ziel, einen strukturierten, auf den Patienten zugeschnittenen Behandlungsplan zu erstellen. Durch all diese
Maßnahmen soll ein multimodales Gesamtkonzept entstehen, das primär zu einem verbesserten Outcome nach elektiven lungenresezierenden Eingriffen und sekundär zu kürzeren
Krankenhausaufenthaltsdauern mit entsprechend geringen Kosten führen soll.Ziele dieses Übersichtsartikels sind neben der Beschreibung grundlegender ERAS-Prinzipien vor allem die kompakte Darstellung der aus Sicht der Autoren wichtigsten europäischen
ERAS-Empfehlungen für lungenresezierende Operationen sowie deren Anwendung bzw. Umsetzung in der deutschen Thoraxchirurgie.
Serum Metabolomic Signature Predicts Ovarian Response to Controlled Stimulation
Authors: Mu Xin , Pei Mei-li , Zhu Feng , Shi Juan Zi , Liu Peijun 
Published: June 2022

AbstractIn in vitro fertilization (IVF), it is meaningful to find novel biomarkers predicting ovarian response in advance. The aim of the study was to identify serum metabolomics predicting ovarian response after controlled ovarian stimulation (COS). Blood samples collected at the start of pituitary downregulation and on the fifth day after COS using Liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods were analyzed to quantify metabolites. Demographic data were calculated with SPSS version 22.0 software. Multivariate statistics were used to analyze metabolomics dataset. A receiver operating characteristic (ROC) curve was used to evaluate the diagnostic model. Analyses revealed 50 different metabolomics between the pre- and post-COS groups. Compared with baseline, amino acids increased significantly following COS. At baseline, acetylglycine was more abundant in FOI<1 group, while glycine and lipids increased in FOI≥1 group. After COS, glycine, N-acetyl-L-alanine, D-alanine, and 2-aminomuconic acid were higher in those with FOI≥1, but L-glutamine was abundant in FOI<1. ROC curves indicated that combination of glycine, acetylglycine, and lipids predicts different responses to COS (AUC=0.866). Serum metabolism might reflect the response to ovarian stimulation. Higher glycine and PC may be a good predictor for response to COS.
Etiology and Outcome of Seizures in Children during Induction Remission Chemotherapy for Acute Lymphoblastic Leukaemia
Authors: Soma Shahinoor A. , Jamal Chowdhury Y. , Chowdhury Indira 
Published: June 2022

AbstractSeizure is one of the most frequent neurological complication and morbid phenomenon among children receiving chemotherapy for acute lymphoblastic leukemia. As overall survival of children with acute lymphoblastic leukemia is improving, now the challenge is to reduce treatment-related adverse effect. However, not much is known about the etiology and natural history of these seizure in our pediatric population. This is a single centered study conducted in the Department of Pediatric Hematology and Oncology, Bangabandhu Sheikh Mujib Medical University. This prospective observational study was conducted over a period of 1 year from May 2017 to April 2018. A total of 105 patients aged 1 year to 17.9 years newly diagnosed as acute lymphoblastic leukemia were the study population. This study showed that in five (33.3%) patients, the underlying cause was suspected intracranial hemorrhage and it was the most common cause. All these five patients had features of severe sepsis and upper motor neuron sign associated with severe thrombocytopenia. Among them three had coagulopathy. Three (20%) patients had CNS leukemic infiltration. Suspected meningitis was attributed as the possible cause of seizure in two (13.33%) patients. Other identifiable causes were brain abscess in one patient, multiple cerebral infarction in one patient, hypertensive encephalopathy in one patient, and vincristine-induced neurotoxicity in one patient. In one patient no identifiable cause was found. Among 15 patients with seizure five (33.3%) patients were improved and completed induction remission chemotherapy. Ten (66.7%) patients died. In this study, we found sepsis and coagulopathy as the major underlying cause of seizure. Outcome was found very dismal in patients who developed seizure.
Comparative Study of Laparoscopic and Open Pyelolithotomy in the Management of Large Renal Pelvic Stones
Authors: Hassan Yaqoob , Rather Ajaz Ahmad , Bashir Arshad , Wani Ishfaq Ahmad , Rasool Humayoon 
Published: June 2022

Abstract
Background Large renal pelvic stones can be effectively managed with laparoscopic pyelolithotomy. The aim of this study was to compare the surgical outcomes of laparoscopic pyelolithotomy versus open surgery for the treatment of large renal pelvic stones.
Materials and Methods This prospective comparative study was performed at the Sher-i-Kashmir Institute of Medical Sciences Medical College and Hospital over a period of 8 years. Using computer-generated random numbers, the patients were randomized into two groups: group A received laparoscopic pyelolithotomy, while group B had open pyelolithotomy. The data was collected and analyzed using SPSS software 22.
Results Among 74 patients who met the inclusion criteria, the mean age was 39.18 years with 66.21% being males and a male:female ratio of 1.96. Forty-one (55.41%) patients had open surgery and 33 (44.59%) had laparoscopic pyelolithotomy. The difference in mean operative time of laparoscopy (117.66 minutes) and open (78.13 minutes) surgery was statistically significant (p = 0.05). The mean blood loss was significantly lower in the laparoscopic pyelolithotomy group (62.12 mL) than in the open group (92.07 mL) (p = 0.009). The difference in mean hospital stay between the open and laparoscopic groups was significant (p = 0.02). In both laparoscopy and open surgery, we observed a 100% stone-free rate at the end of 1 month. None of our patients expired during the study period.
Conclusion Laparoscopic pyelolithotomy is a promising alternative to traditional open and other endourological techniques, with encouraging results. Despite its technical difficulty, it yields high stone-free rates and low postoperative morbidity.
Outcomes of Triple-Negative Breast Cancer Patients in Response to Taxane and Nontaxane-Based Neoadjuvant Chemotherapies
Authors: Khan Mohsin , Alam Zarmina , Siddiqui Shahid Ali , Akram Mohd , Alam Mohd Shadab 
Published: June 2022

Abstract
Introduction Neoadjuvant chemotherapy (NAC) is the standard of care for locally advanced breast cancer. The achievement of pathological complete response (pCR) in response to NAC is of clinical significance as it correlates with improved long-term outcome. pCR rate varies with different chemotherapeutic agents and a higher rate may be associated with higher treatment cost and more toxicity.Triple-negative breast cancer (TNBC) has poorer prognosis than non-TNBC (estrogen receptor, progesterone receptor, and/or human epidermal growth factor receptor 2 expressing tumors), but patients achieving pCR may have similar outcome. We evaluated the response of TNBC after taxane and nontaxane-based NAC and its correlation with survival outcome.
Materials and Methods This was a retrospective study comparing the efficacy of “taxotere, Adriamycin and cyclophosphamide TAC” versus “cyclophosphamide, epirubicin, 5-fluorouracil/cyclophosphamide, Adriamycin, 5-fluorouracil (CEF/CAF)” NAC regimens in patients with locally advanced TNBC. The efficacy and safety of both the regimens were compared. Overall and disease-free survival were analyzed.
Results Two-hundred and forty-nine eligible patients were included and divided into two groups: taxane group receiving “TAC” regimen (123 patients) and nontaxane group receiving “CEF/CAF” regimen (126 patients). A higher pCR rate was achieved with taxane compared with nontaxane NAC (28 vs. 12%) (p = 0.0001). Although taxane addition did not lead to survival advantage for the entire group, significantly better survival rates were achieved for patients who had pCR compared with similar subgroup of patients in nontaxane group. Both the NAC regimens were well tolerable.
Conclusion Taxane-based NAC was although costlier than anthracycline-based regimen but was more effective, resulting in a higher pCR rate and an improved survival outcome in patients who achieved pCR.