bluuebunny/crossref_metadata_2025_split huggingface dataset:
Medici Rule Reimagined: Cosimo iii, the Dutch Republic, and Grand Ducal Aspirations for Seventeenth-Century Tuscany (c. 1667–1723)
Authors: van Vugt Ingeborg , Moorman Gloria 
Published: December 2022

Abstract
The enticingly modern strain of republicanism that young Prince Cosimo iii de’ Medici (1642–1723) encountered during his two sojourns in the Dutch Republic (1667–1669) proved a forceful means to reimagine Tuscany’s own, administrative past and present. Through comparative analysis of the unpublished travel journal of Medici secretary Apollonio Bassetti (1631–1699) and the diary in verse by court physician Giovanni Andrea Moniglia (1624–1700), we argue that Cosimo iii’s ambitious agenda abroad was influenced predominantly by his desire to implement environmental reform and portray a contrasting socio-political model at home. Cosimo’s own journeys were followed by ongoing transnational exchange, as testified by the court’s efforts to conceptualize a Medici town atlas and cultivate exotic pineapple plants on the Tuscan soil. By importing artefacts and ideas, then, Cosimo iii – just prior to his succession by Gian Gastone (1671–1737), last of the Medici grand dukes – sought to consciously craft the Medici dynasty’s lasting legacy.
Distance to Dislocation and Recurrent Shoulder Dislocation After Arthroscopic Bankart Repair: Rethinking the Glenoid Track Concept
Authors: Barrow Aaron E. , Charles Shaquille J.-C. , Issa Mohamad , Rai Ajinkya A. , Hughes Jonathan D. , Lesniak Bryson P. , Rodosky Mark W. , Popchak Adam , Lin Albert 
Published: December 2022

Background: The “distance to dislocation” (DTD) calculation has been proposed as 1 method to predict the risk of recurrent dislocation after arthroscopic Bankart repair for an “on-track” shoulder. Rates of recurrent dislocation at specific DTD values are unknown. Hypothesis: Among patients with “on-track” shoulder lesions who underwent primary arthroscopic Bankart repair, the rate of recurrent dislocation would increase as DTD values decrease. Study Design: Case-control study; Level of evidence, 3. Methods: We performed a retrospective analysis of 188 patients with “on-track” shoulder lesions who underwent primary arthroscopic anterior labral repair between 2007 and 2019, with a minimum 2-year follow-up. Glenoid bone loss, Hill-Sachs interval, glenoid track, and DTD were determined from preoperative magnetic resonance imaging scans. The rate of recurrent dislocation was determined at 2-mm DTD intervals. Univariate and multivariate regression analyses were used to evaluate the relationship between recurrent dislocation, patient characteristics, and bone loss variables. A multivariate regression model was created to predict the probability of failure at continuous DTD values. A subgroup analysis of failure rate based on collision sports participation was also performed. Results: A total of 29 patients (15.4%) sustained recurrent dislocations. Patient age ( P = .046), multiple dislocations ( P = .03), glenoid bone loss ( P < .001), Hill-Sachs interval length ( P < .001), and DTD ( P < .001) were all independent predictors of failure. As the DTD decreased, the rate of recurrent dislocation increased. Below a DTD threshold of 10 mm, the recurrent dislocation rate increased exponentially. Up to a threshold of 24 mm, the failure rate for collision athletes remained >12.3%, independent of the DTD. Conversely, the failure rate among noncollision athletes decreased steadily as the DTD increased. Conclusion: For “on-track” shoulder lesions, as the DTD approached 0 mm (“off-track” threshold), the risk of recurrent dislocation after arthroscopic Bankart repair increased significantly. Below a DTD threshold of 10 mm, the risk of failure increased exponentially. The risk of recurrent dislocation for collision sports athletes remained elevated at higher DTD values than for noncollision athletes.
Low-frequency vibration suppression of metastructure beam with high-static–low-dynamic stiffness resonators employing magnetic spring
Authors: Peng Fanglan , Wu Qichen , Pan Wuhui , Zheng Yisheng , Xie Shilin , Zhang Yahong , Luo Yajun 
Published: December 2022

The low/ultra-low-frequency structural vibrations exist widely in some engineering structures, for example, aerospace, naval, and building structures and so on. They could seriously affect the working performance and even cause the destruction of these engineering structures. In order to restrain the low/ultra-low-frequency structural vibration, a locally resonant (LR) metastructure beam equipped with high-static–low-dynamic stiffness (HSLDS) resonators employing negative stiffness magnetic spring is proposed. The analytical model of magnetic negative stiffness spring is firstly derived and the design method of HSLDS resonators is presented based on parametric optimization. Then, the dynamic model of the LR metastructure beam with HSLDS resonators is established using the wave finite element method. The effects of the number of local resonator units on low-frequency band gap are analyzed. Finally, the low-frequency vibration control performance of LR metastructure beam is validated experimentally. The experimental result is in agreement with the theoretical analysis, which demonstrates that the HSLDS resonators are conductive to suppress vibration of the metastructure beam in the low-frequency region ( f < 10 Hz). The results also showed that the more the HSLDS resonators are used, the better the vibration suppression effect is.
Exosomal miR-628-5p from M1 polarized macrophages hinders m6A modification of circFUT8 to suppress hepatocellular carcinoma progression
Authors: Wang Liyan , Yi Xiaoyuan , Xiao Xuhua , Zheng Qinghua , Ma Lei , Li Bin 
Published: December 2022

Abstract
Background
Hepatocellular carcinoma (HCC) is the most common type of liver cancer. CircFUT8 has been shown to be upregulated in cancers, but its function in HCC remains unclear. Tumor-associated macrophages (TAMs) are one of the main components of the tumor microenvironment (TME), and M1 macrophages function as tumor suppressors in cancers. Exosomes exert an important role in the TME, and circRNAs can be modified by m6A. We investigated the function of circFUT8 in HCC and its interaction with exosomes, M1 macrophages, and m6A.

Methods
CircFUT8 expression was detected in HCC cells, and its effects on HCC cell growth were verified through functional assays. Mechanism assays including RNA pull down, RNA-binding protein immunoprecipitation (RIP), and luciferase reporter assays were undertaken to verify how circFUT8 may interact with miR-628-5p, and how these molecules may modulate HCC cell malignancy via interacting with exosomes and macrophages.

Results
CircFUT8 was upregulated in HCC cells and it accelerated HCC cell growth. Exosomes derived from M1 macrophages transferred miR-628-5p to HCC cells to inhibit human methyltransferase-like 14 (METTL14) expression. METTL14 promoted circFUT8 m6A modification and facilitated its nuclear export to the cytoplasm, where M1 macrophages regulated the circFUT8/miR-552-3p/CHMP4B pathway, thereby suppressing HCC progression.

Conclusion
M1 macrophages-derived exosomal miR-628-5p inhibited the m6A modification of circFUT8, inhibiting HCC development.

Graphical Abstract
Reducing 48-h emergency department revisits and subsequent admissions: a retrospective study of increased emergency medicine resident floor coverage
Authors: Palungwachira Pakhawadee , Montimanutt Gunnaree , Musikatavorn Khrongwong , Savatmongkorngul Sorravit 
Published: December 2022

AbstractBackgroundEarly unexpected hospital admission after emergency department (ED) discharge is an important topic regarding effective preventive measures. Reducing avoidable return visits can improve ED effectiveness and emergency care. This study evaluated the effects of an increase in the number of physicians and the 24-h coverage of emergency physicians on 48-h ED revisits with subsequent hospital admission. The characteristics and risk factors of the patients were also investigated.ResultsThis was a retrospective analysis performed 2 years before and 2 years after the implementation of an intervention in a tertiary care hospital in Thailand. The medical records of adult patients who revisited the ED within 48 h for related complaints were reviewed. The effect of the intervention was analyzed, and a prediction model was developed based on logistic regression.After implementing the intervention, the hospital admission rate at the second ED visit decreased from 44.5 to 41.1%; no significant difference was found (95% confidence interval (CI) − 5.05 to 11.78). Patients who required hospital admission had a significantly higher comorbidity score, more ED visits, and more hospitalizations within the past 12 months. A significantly higher hospital admission rate was also observed among patients older than 60 years, those who had an initial infectious diagnosis, and those who had a higher triage severity level (ESI II) at their first visit. The odds ratio (OR) showed lower odds of hospital admission at the second visit in the postintervention period; this difference was not significant (OR 0.87; 95% CI 0.61 to 1.23).ConclusionOur intervention did not significantly decrease the incidence of admission at an ED revisit. However, some factors identified in this study seem to have some benefits and might be helpful for preventing errors and constructing a standard discharge care plan for patients with these risk factors.
Genome-wide identification and stress response analysis of cyclophilin gene family in apple (Malus × domestica)
Authors: Qiao Zhi-Wen , Wang Da-Ru , Wang Xun , You Chun-Xiang , Wang Xiao-Fei 
Published: December 2022

AbstractBackgroundCyclophilin (CYP) belongs to the immunophilin family and has peptidyl-prolylcis-transisomerase (PPIase) activity, which catalyzes thecis-transisomerization process of proline residues. CYPs widely exist in eukaryotes and prokaryotes, and contain a conserved cyclophilin-like domain (CLD). Plant cyclophilins are widely involved in a range of biological processes including stress response, metabolic regulation, and growth and development.ResultIn this study, 30 cyclophilin genes on 15 chromosomes were identified from the ‘Golden Delicious’ apple (M. domestica) genome. Phylogenetic analysis showed that the cyclophilin family genes can be divided into three clades inMalus. Collinear analysis showed that ten gene pairs were the result of segmental duplication. Analysis of gene and protein structure further supported the phylogenetic tree and collinearity analysis. The expression ofMdCYPsin different organs was higher in leaves, flowers, and fruits. Ten and eight CYPs responded to drought and salt stress, respectively. MdCYP16, a nuclear-localized MD CYP, was screened from the intersection of the two expression profiling datasets and was highly sensitive to drought and salt stress. GUS staining of transgenicArabidopsisindicated thatMdCYP16may be involved in the regulation of abiotic stress.ConclusionThis study systematically analyzed members of the apple cyclophilin family and confirmed the involvement ofMdCYP16as a nuclear-localized MD cyclophilin that acts in response to salt and drought stress in apple. Our work identifies members of the apple cyclophilin gene family, and provides an important theoretical basis for in-depth study of cyclophilin function. Additionally, the analysis provides candidate genes that may be involved in stress response in apple.
Fine mapping of CscpFtsY, a gene conferring the yellow leaf phenotype in cucumber (Cucumis sativus L.)
Authors: Zha Gaohui , Yin Juan , Cheng Feng , Song Mengfei , Zhang Mengru , Obel Hesbon Ochieng , Wang Yi , Chen Jinfeng , Lou Qunfeng 
Published: December 2022

Abstract
Background
Leaf color mutants are ideal materials to study pigment metabolism and photosynthesis. Leaf color variations are mainly affected by chlorophylls (Chls) and carotenoid contents and chloroplast development in higher plants. However, the regulation of chlorophyll metabolism remains poorly understood in many plant species. The chloroplast signal-recognition particle system is responsible for the insertion of the light-harvesting chlorophyll a/b proteins (LHCPs) to thylakoid membranes, which controls the chloroplast development as well as the regulation of Chls biosynthesis post-translationally in higher plants.

Results
In this study, the yellow leaf cucumber mutant, named yl, was found in an EMS-induced mutant library, which exhibited a significantly reduced chlorophyll content, abnormal chloroplast ultrastructure and decreased photosynthetic capacity. Genetic analysis demonstrated that the phenotype of yl was controlled by a recessive nuclear gene. Using BSA-seq technology combined with the map-based cloning method, we narrowed the locus to a 100 kb interval in chromosome 3. Linkage analysis and allelism test validated the candidate SNP residing in CsaV3_3G009150 encoding one homolog of chloroplast signal-recognition particle (cpSRP) receptor in Arabidopsis, cpFtsY, could be responsible for the yellow leaf phenotype of yl. The relative expression of CscpFtsY was significantly down-regulated in different organs except for the stem, of yl compared with that in the wild type (WT). Subcellular localization result showed that CscpFtsY located in the chloroplasts of mesophyll cells.

Conclusions
The yl mutant displayed Chls-deficient, impaired chloroplast ultrastructure with intermittent grana stacks and significantly decreased photosynthetic capacity. The isolation of CscpFtsY in cucumber could accelerate the progress on chloroplast development by cpSRP-dependant LHCP delivery system and regulation of Chls biosynthesis in a post-translational way.
Identification and validation of plant height, spike length and spike compactness loci in common wheat (Triticum aestivum L.)
Authors: Liu Hong , Shi Zhipeng , Ma Feifei , Xu Yunfeng , Han Guohao , Zhang Jinpeng , Liu Dongcheng , An Diaoguo 
Published: December 2022

Abstract

Background
Plant height (PH), spike length (SL) and spike compactness (SCN) are important agronomic traits in wheat due to their strong correlations with lodging and yield. Thus, dissection of their genetic basis is essential for the improvement of plant architecture and yield potential in wheat breeding. The objective of this study was to map quantitative trait loci (QTL) for PH, SL and SCN in a recombinant inbred line (RIL) population derived from the cross ‘PuBing3228 × Gao8901’ (PG-RIL) and to evaluate the potential values of these QTL to improve yield.

Results
In the current study, Five, six and ten stable QTL for PH, SL, and SCN, respectively, were identified in at least two individual environments. Five major QTL QPh.cas-5A.3, QPh.cas-6A, QSl.cas-6B.2, QScn.cas-2B.2 and QScn.cas-6B explained 5.58–25.68% of the phenotypic variation. Notably, two, three and three novel stable QTL for PH, SL and SCN were identified in this study, which could provide further insights into the genetic factors that shape PH and spike morphology in wheat. Conditional QTL analysis revealed that QTL for SCN were mainly affected by SL. Moreover, a Kompetitive Allele Specific PCR (KASP) marker tightly linked to stable major QTL QPh.cas-5A.3 was developed and verified using the PG-RIL population and a natural population.

Conclusions
Twenty-one stable QTL related to PH, SL, and SCN were identified. These stable QTL and the user-friendly marker KASP8750 will facilitate future studies involving positional cloning and marker-assisted selection in breeding.
Is prehospital intubation of severely injured children in accordance with guidelines?
Authors: Maek Teresa , Fochtmann Ulrike , von Loewenich Anna , Jungbluth Pascal , Zimmermann Werner , Lefering Rolf , Lendemans Sven , Hussmann Bjoern 
Published: December 2022

Abstract
Background
The current German S3 guideline for polytrauma lists five criteria for prehospital intubation: apnea, severe traumatic brain injury (GCS ≤8), severe chest trauma with respiratory failure, hypoxia, and persistent hemodynamic instability. These guideline criteria, used in adults in daily practice, have not been previously studied in a collection of severely injured children. The aim of this study was to assess the extent to which the criteria are implemented in clinical practice using a multivariate risk analysis of severely injured children.

Methods
Data of 289,698 patients from the TraumaRegister DGU® were analyzed. Children meeting the following criteria were included: Maximum Abbreviated Injury Scale 3+, primary admission, German-speaking countries, years 2008–2017, and declaration of intubation. Since children show age-dependent deviating physiology, four age groups were defined (years old: 0–2; 3–6; 7–11; 12–15). An adult collective served as a control group (age: 20–50). After a descriptive analysis in the first step, factors leading to prehospital intubation in severely injured children were analyzed with a multivariate regression analysis.

Results
A total of 4489 children met the inclusion criteria. In this cohort, young children up to 2 years old had the significantly highest injury severity (Injury Severity Score: 21; p ≤ 0.001). Falls from both high (> 3 m) and low heights (< 3 m) were more common in children than in adults. The same finding applied to the occurrence of severe traumatic brain injury. When at least one intubation criterion was formally present, the group up to 6 years old was least likely to actually be intubated (61.4%; p ≤ 0.001). Multivariate regression analysis showed that Glasgow Coma Scale score ≤ 8 in particular had the greatest influence on intubation (odds ratio: 26.9; p ≤ 0.001).

Conclusions
The data presented here show for the first time that the existing criteria in the guideline for prehospital intubation are applied in clinical practice (approximately 70% of cases), compared to adults, in the vast majority of injured children. Although severely injured children still represent a minority of all injured patients, future guidelines should focus more on them and address them in a specialized manner.
Generating real-world evidence compatible with evidence from randomized controlled trials: a novel observational study design applicable to surgical transfusion research
Authors: Yu Xiaochu , Wang Zixing , Wang Lei , Huang Yuguang , Wang Yipeng , Xin Shijie , Lei Guanghua , Zhao Shengxiu , Chen Yali , Guo Xiaobo , Han Wei , Yu Xuerong , Xue Fang , Wu Peng , Gu Wentao , Jiang Jingmei 
Published: December 2022

Abstract
Background
Numerous observational studies have revealed an increased risk of death and complications with transfusion, but this observation has not been confirmed in randomized controlled trials (RCTs). The “transfusion kills patients” paradox persists in real-world observational studies despite application of analytic methods such as propensity-score matching. We propose a new design to address this long-term existing issue, which if left unresolved, will be deleterious to the healthy generation of evidence that supports optimized transfusion practice.

Methods
In the new design, we stress three aspects for reconciling observational studies and RCTs on transfusion safety: (1) re-definition of the study population according to a stable hemoglobin range (gray zone of transfusion decision; 7.5–9.5 g/dL in this study); (2) selection of comparison groups according to a trigger value (last hemoglobin measurement before transfusion; nadir during hospital stay for control); (3) dealing with patient heterogeneity according to standardized mean difference (SMD) values. We applied the new design to hospitalized older patients (aged ≥60 years) undergoing general surgery at four academic/teaching hospitals. Four datasets were analyzed: a base population before (Base Match−) and after (Base Match+) propensity-score matching to simulate previous observational studies; a study population before (Study Match−) and after (Study Match+) propensity-score matching to demonstrate effects of our design.

Results
Of 6141 older patients, 662 (10.78%) were transfused and showed high heterogeneity compared with those not receiving transfusion, particularly regarding preoperative hemoglobin (mean: 11.0 vs. 13.5 g/dL) and intraoperative bleeding (≥500 mL: 37.9% vs. 2.1%). Patient heterogeneity was reduced with the new design; SMD of the two variables was reduced from approximately 100% (Base Match−) to 0% (Study Match+). Transfusion was related to a higher risk of death and complications in Base Match− (odds ratio [OR], 95% confidence interval [CI]: 2.68, 1.86–3.86) and Base Match+ (2.24, 1.43–3.49), but not in Study Match− (0.77, 0.32–1.86) or Study Match+ (0.66, 0.23–1.89).

Conclusions
We show how choice of study population and analysis could affect real-world study findings. Our results following the new design are in accordance with relevant RCTs, highlighting its value in accelerating the pace of transfusion evidence generation and generalization.
Association of circulating tumor cell-white blood cell clusters with survival outcomes in patients with colorectal cancer after curative intent surgery
Authors: Xu Yifan , Zhang Qianlong , Xu Zhou , Xie Qingfeng , Ding Wenfu , Liu Hao , Deng Haijun 
Published: December 2022

Abstract
Background
The analysis of circulating tumor cell-associated white blood cell (CTC-WBC) clusters represented the progress in the liquid biopsy of malignant tumors, however, related research in patients with colorectal cancer is still absent.

Methods
To explore associations between CTC-WBC clusters and the prognosis of these patients, we conducted an independent cohort of 329 colorectal cancer patients after curative intent surgery and pre-operative CTC detection in Nanfang Hospital, Southern Medical University, Guangzhou, China between January 1, 2017, and September 31, 2019. The primary cohort referred to patients with CTC-WBC clusters positive. The control cohort was defined as those with exclusively CTCs positive. CTCs were enriched and distinguished by The CanPatrol™ system (SurExam, China). The Kaplan–Meier curve was used to compare the progressive-free survival (PFS) and overall survival (OS) between two groups. The COX regression model was used to assess the predictive value of CTC-WBC clusters.

Results
Sixty three patients presented CTC-WBC clusters positive (CTC-WBC group) and 266 patients showed solely CTCs (CTC group). The number of CTCs was significantly different between two groups (P < 0.001) and the rest of clinical characteristics were not markedly associated with the presence of CTC-WBC clusters. Kaplan–Meier curves of PFS and OS exhibited that the CTC-WBC group had significantly shorter PFS (P = 0.011), while not for OS. The multivariate model further suggested that the CTC-WBC clusters (Hazard Ratio = 1.89, 95% Confidence Interval 1.02–3.51, P = 0.042) was an independent predictor for the PFS of in post-operation CRC patients.

Conclusion
The CTC-WBC cluster is significantly associated with recurrence after operation in CRC patients. This finding facilitates the evaluation of this indicator in tumor progression.