bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of communicative and critical health literacy on trust in physicians among patients with systemic lupus erythematosus: the TRUMP2-SLE project
Authors: Oguro Nao , Yajima Nobuyuki , Miyawaki Yoshia , Yoshimi Ryusuke , Shimojima Yasuhiro , Sada Ken-ei , Hayashi Keigo , Shidahara Kenta , Sakurai Natsuki , Hidekawa Chiharu , Kishida Dai , Ichikawa Takanori , Ishikawa Yuichi , Kurita Noriaki 
Published: May 2022

ABSTRACTObjectivesAccessing the Internet has increased the gap in patient health literacy (HL), impacting patient-doctor trust. We examined how trust in physicians is affected by functional HL (the ability to read and write) and by broader concepts of HL, including communicative HL (the ability to extract information from communication to use) and critical HL (the ability to analyze and use information) among patients with systemic lupus erythematosus (SLE).MethodsThis cross-sectional study enrolled 362 SLE patients at five academic centers between June 2020 and August 2021. The 14-item Functional Communicative Critical Health Literacy Scale assessed the three dimensions of HL (range: 1-4 points). Outcomes were trust in one’s physician and physicians generally using the 5-item Wake Forest Physician Trust Scale (range: 0-100 points). General linear models adjusted for age, sex, education, income, disease activity, disease duration, depression, and time using the Internet.ResultsTrust in one’s physician increased with higher functional and communicative HL (per 1-pt increase, 3.21 [95%CI 0.61, 5.81], 5.8 [95%CI 1.96, 9.63]). Trust in physicians in general increased with higher communicative HL and decreased with higher critical HL (per 1-pt increase, 7.01 [95%CI 2.27, 11.76], -6.83 [95%CI -11.67, -1.99]). Longer Internet use was associated with both higher communicative and critical HL.ConclusionsOur findings suggest that rheumatologists can help patients build trust by encouraging dialogue about their health issues with their doctors and family members, rather than trying to improve their ability to discern health information.Key messagesWhat is already known on this topicFactors affecting trust in one’s physician among chronic diseases including SLE are economic status, misdiagnosis experience, and the duration of the physician-patient relationship.In the general population, higher trust in physicians generally was associated with functional HL as patients’ external factors.What this study addsThis is the first study to reveal an association between trust in one’s own physician and HL among patients with SLE. In addition, we were able to expand the correlates of HL by measuring broader concepts of HL dimensions, such as communicative and critical HL.How this study may affect research, practice, or policyThis study suggests the importance of rheumatologists’ encouragement of patients to communicate, share their concerns, and resolve their misunderstandings to develop a trusting relationship. This study recommends that communictive HL (ability to obtain useful health information) should be the focus and not critical HL (ability to discern health information).
Arbuscular mycorrhizae reduce stress-induced plasticity of plant functional traits. A meta-analysis study
Authors: Globbo Florencia , Corriale María José , Gázquez Ayelén , Bordenave César Daniel , Bilenca David , Menéndez Ana 
Published: May 2022

AbstractWe aimed at exploring the plant functional traits whose stress-induced plasticity is altered by the presence of AM fungi, considering the direction of their changes. We also sought for a coordinated variation of plant biomass and functional traits, during plant adaptation to environmental stressors, and the role of AM status on the variation. We performed a meta-analysis across 114 articles spanning 110 plant species or cultivars. We quantified the size effect of AM symbiosis on the stress-induced plasticity of several reported and calculated functional traits, and using linear mixed model analysis (LMM). Correlation between traits plasticity and total biomass variation were also performed through LMM. The literature search and further selection yielded seven functional traits, extracted from 114 laboratory studies, including 888 observations and 110 plant species/cultivars. Evidence for significant effects of predictor variables (type of stress, AM symbiosis and/or their interaction) on plasticity were found for three of these functional traits: leaf-area ratio (LAR), root mass fraction (RMF) and root-shoot (R:S) ratio. Our results provided evidence to accept the hypothesis that AM fungal inoculation may reduce the phenotypic plasticity of important plant functional traits leaf area ratio (LAR), root mass fraction (RMF) and root-shoot (R:S) ratio, by decreasing its magnitude. We also found a weak correlation between traits plasticity and total biomass variation. Although our literature search and data collection were intensive and our results robust, the scope of our conclusions is limited by the agronomical bias of plant species targeted by the meta-analysis. Further knowledge on non-cultivable plant species and better understanding of the mechanisms ruling resources allocation in plants would allow more generalized conclusions.
Improved diagnosis of inflammatory bowel disease and prediction and monitoring of response to anti-TNF alpha treatment based on measurement of signal transduction pathway activity
Authors: Bouwman Wilbert , Verhaegh Wim , van de Stolpe Anja 
Published: May 2022

AbstractObjectiveUlcerative colitis (UC) and Crohn’s disease (CD) are two subtypes of chronic inflammatory bowel disease (IBD). Differential diagnosis remains a challenge. Anti-TNFα treatment is an important treatment for IBD, yet resistance frequently occurs and cannot be predicted. Consequently, many patients receive ineffective therapy with potentially adverse effects. Novel assays are needed to improve diagnosis, and predict and monitor response to anti-TNF-α compounds.DesignSignal transduction pathway (STP) technology was used to quantify activity of STPs (androgen and estrogen receptor, PI3K, MAPK, TGFβ, Notch, Hedgehog, Wnt, NFκB, JAK-STAT1/2, and JAK-STAT3 pathways) in colon mucosa samples of CD and UC patients, based on transcriptome analysis. Previously described STP assay technology is based on computational inference of STP activity from mRNA levels of target genes of the STP transcription factor.ResultsResults show that NFκB, JAK-STAT3, Wnt, MAPK, and androgen receptor pathways were abnormally active in CD and UC. Colon and ileum-localized CD differed with respect to STP activity, the JAK-STAT1/2 pathway being abnormally active in ileal CD. High activity of NFκB, JAK-STAT3, and TGFβ pathways was associated with resistance to anti-TNFα treatment in UC and colon-located CD, but not in ileal CD. STP activity decreased with successful treatment.ConclusionWe believe that measuring mucosal STP activity provides clinically relevant information to improve differential diagnosis of IBD and prediction of resistance to anti-TNFα treatment in patients with colon-localized IBD, and provides new targets for treatment and overcoming anti-TNFα resistance.
In vitro and in vivo gene introduction in the cloudy catshark (Scyliorhinus torazame), a cartilaginous fish
Authors: Fujimori Chika , Umatani Chie , Chimura Misaki , Ijiri Shigeho , Bando Hisanori , Hyodo Susumu , Kanda Shinji 
Published: May 2022

AbstractCartilaginous fishes have various unique physiological features such as cartilaginous skeletons and a urea-based osmoregulation strategy for adaptation to their marine environment. Also, because they are considered a sister group of bony vertebrates, understanding their unique features is important from an evolutionary perspective. However, experimental approaches are limited in cartilaginous fishes. Particularly, genetic engineering, which can analyze gene functions as well as cellular behavior, has not been effectively utilized in cartilaginous fishes. This is partly because their reproductive strategy involves internal fertilization, which results in difficulty in microinjection into fertilized eggs at the early developmental stage. Trials of gene transfer have also been limited both in in vitro cultured cells and in vivo. Here, to identify efficient gene transfer methods in cartilaginous fishes, we examined the effects of various methods both in vitro and in vivo using the cloudy catshark, a candidate model cartilaginous fish species. In all methods, green fluorescent protein (GFP) expression was used to evaluate exogenous gene introduction. First, we established a primary cell culture containing fibroblast-like and epithelial-like cells from cloudy catshark embryos. Using these primary cultured cells, we attempted gene transfection by lipofection, polyethylenimine (PEI), adenovirus, baculovirus and electroporation. Among the methods tested, lipofection, electroporation and baculovirus infection enabled the successful introduction of exogenous genes into primary cultured cells, allowing us to study physiological mechanisms at a single-cell level in culture conditions close to those in a living cartilaginous fish. We also attempted in vivo transfection into cloudy catshark embryos by electroporation and baculovirus infection. Although baculovirus-injected groups did not show GFP fluorescence, electroporation successfully introduced GFP into various tissues including muscle cells. Furthermore, we succeeded in GFP introduction into adult testis by electroporation. The in vitro and in vivo gene introduction methods that worked in this study may identify paths for future genetic manipulation including knockout experiments and cellular linage analysis in cartilaginous fishes.
Improved SpCas9 and LbCas12a genome editing systems in Brassica oleracea and Brassica napus
Authors: Lawrenson Tom , Chhetry Monika , Clarke Martha , Hundleby Penny , Harwood Wendy 
Published: May 2022

AbstractWe report highly efficient genome editing in Brassica species. We compare the efficiency of targeted mutagenesis using four Streptococcus pyogenes Cas9 (SpCas9) systems in Brassica oleracea and Brassica napus over 3 target genes and five guides to identify two which show a striking improvement to our first published system (Lawrenson et al., 2015). Targeted mutagenesis occurred in up to 100% of T0 plants with the improved systems, compared to 20% in the original system. This is the only reported comparison of SpCas9 systems we are aware of in Brassica species.Secondly, we report the first successful use of Lachnospiraceae bacterium Cas12a (LbCas12a) in Brassica oleracea. We test three LbCas12a coding sequences and two guide architectures against one target gene using four guides. From this we identify the best performing combination of our novel, multi-intron coding sequence and a ribozyme flanked guide expression cassette. In this case 68% of T0 plants contained targeted mutations. Heritability of LbCas12a mutations is shown. We show that our two useful and novel LbCas12a coding sequences have utility in Brassica species.
Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly
Authors: Fischer Elyse S. , Yu Conny W.H. , Hevler Johannes F. , McLaughlin Stephen H. , Maslen Sarah L. , Heck Albert J.R. , Freund Stefan M.V. , Barford David 
Published: May 2022

AbstractIn response to improper kinetochore-microtubule attachments in mitosis, the spindle assembly checkpoint (SAC) assembles the mitotic checkpoint complex (MCC) to inhibit the anaphase- promoting complex/cyclosome, thereby delaying entry into anaphase. The MCC is a complex of Mad2, Cdc20 and BubR1:Bub3. Its assembly is catalysed by unattached kinetochores on a catalytic Mad1:Mad2 platform. Mad1-bound closed-Mad2 (C-Mad2) recruits open-Mad2 (O- Mad2) through self-dimerization. This interaction, combined with Mps1 kinase-mediated phosphorylation of Bub1 and Mad1, accelerates MCC assembly, in a process that requires conversion of O-Mad2 to C-Mad2 and concomitant binding of Cdc20. How Mad1 phosphorylation catalyses MCC assembly is poorly understood. In this study we characterized Mps1 phosphorylation of Mad1 and obtained structural insights into a phosphorylation-specific Mad1:Cdc20 interaction. This interaction, together with the Mps1-phosphorylation dependent association of Bub1 and Mad1, generates a tripartite assembly of Bub1 and Cdc20 onto the C- terminal domain of Mad1 (Mad1CTD). We additionally identified a folded state of the Mad1:Mad2 complex that suggests how the Cdc20:Mad1CTDinteraction brings the Mad2- interacting motif (MIM) of Cdc20 (Cdc20MIM) into close proximity with O-Mad2. We also show that Cdc20MIMis sufficient to catalyse Mad2 conversion and its entrapment by the Mad2 safety- belt. Thus, Mps1-dependent formation of the MCC-assembly scaffold functions to position and orient Cdc20MIMnear O-Mad2, thereby catalysing formation of C-Mad2:Cdc20.
The L1 Cell Adhesion Molecule Constrains Dendritic Spine Density through Ankyrin Binding in Pyramidal Neurons of the Mouse Cerebral Cortex
Authors: Murphy Kelsey E. , Wade Sarah D. , Sperringer Justin E. , Mohan Vishwa , Duncan Bryce W. , Pak Yubin , Lutz David , Schachner Melitta , Maness Patricia F. 
Published: May 2022

AbstractA novel function for L1 cell adhesion molecule and its interaction with Ankyrin, an actin-spectrin adaptor protein, was identified in constraining dendritic spine density on pyramidal neurons in the mouse neocortex. In an L1-null mouse mutant increased spine density was observed on apical but not basal dendrites of pyramidal neurons in diverse cortical areas (prefrontal cortex layer 2/3, motor cortex layer 5, visual cortex layer 4).The Ankyrin binding motif (FIGQY) in L1’s cytoplasmic domain was critical for spine formation, as demonstrated by increased spine density in the prefrontal cortex of a mouse mutant (L1YH) harboring a tyrosine to histidine mutation in this motif, which disrupts L1-Ankyrin association. This mutation is a known variant in the human L1 syndrome. In both mutants mature mushroom spines rather than immature spines were predominant. L1 was detected in spines and dendrites of wild-type prefrontal cortical neurons by immmunostaining. L1 coimmunoprecipitated with Ankyrin B (220 kDa) from cortical lysates of wild-type but not L1YH mice. Spine pruning assays in cortical neuron cultures from wild-type and L1YH mutant mice showed that the L1-Ankyrin interaction mediated spine retraction in response to the class 3 Semaphorins, Sema3F and to a lesser extent Sema3B. These ligands also induce spine pruning through other L1 family adhesion molecules, NrCAM and Close Homolog of L1 (CHL1), respectively. This study provides insight into the molecular mechanism of spine regulation and underscore the potential for this adhesion molecule to regulate cognitive and other L1-related functions that are abnormal in the L1 syndrome.
MFGE8 links absorption of dietary fatty acids with catabolism of enterocyte lipid stores through HNF4γ-dependent transcription of CES enzymes
Authors: Datta Ritwik , Gholampour Mohammad A , Yang Christopher D , Volk Regan , Lin Sinan , Podolsky Michael J , Arnold Thomas , Rieder Florian , Zaro Balyn W. , Verzi Michael , Lehner Richard , Abumrad Nada , Lizama Carlos O , Atabai Kamran 
Published: May 2022

ABSTRACTEnterocytes modulate the extent of postprandial lipemia, a potent risk factor for developing atherosclerotic disease, by storing dietary fats in cytoplasmic lipid droplets (cLDs). We have previously demonstrated that the integrin ligand MFGE8 links absorption of dietary fats with activation of triglyceride (TG) hydrolases that catabolize cLDs for chylomicron production. The hydrolase(s) responsible for mobilization of TG from diet-derived cLDs is unknown though recent evidence indicates that this process is independent of the canonical pathway of TG hydrolysis mediated by ATGL. Here we identify CES1D as the key hydrolase downstream of the MFGE8-αvβ5 integrin pathway that regulates catabolism of diet-drive cLDs. Mfge8 KO enterocytes have reduced CES1D transcript and protein levels and reduced protein levels of the transcription factor HNF4γ. Mice KO for Ces1d or Hnf4γ have decreased enterocyte TG hydrolase activity coupled with retention of TG in cLDs. Mechanistically, MFGE8-dependent fatty acid uptake through CD36 leads to stabilization of HNF4γ protein levels; HNF4γ then increases Ces1d transcription. Our work identifies a regulatory network by which MFGE8 and αvβ5 regulate the severity of postprandial lipemia by linking dietary fat absorption with protein stabilization of a transcription factor that increases expression of enterocyte TG hydrolases that catabolize diet-derived cLDs.
Integration of multi-omics data shows downregulation of mismatch repair, purin, and tublin pathways in AR-negative triple-negative chemotherapy-resistant breast tumors
Authors: Tang Xiaojia , Thompson Kevin J. , Kalari Krishna R. , Sinnwell Jason P. , Suman Vera J. , Vedell Peter T. , McLaughlin Sarah A. , Northfelt Donald W. , Aspitia Alvaro Moreno , Gray Richard J. , Carter Jodi , Weinshilboum Richard , Wang Liewei , Boughey Judy C. , Goetz Matthew P. 
Published: May 2022

ABSTRACTTriple negative breast cancer (TNBC) patients who fail to achieve a pathological complete response to neoadjuvant chemotherapy (NAC) will likely experience recurrence of the disease within 3-4 years. Prognostic assessment of early recurrence could facilitate clinical decisions and impact disease survival. This study investigated pre- and post-NAC multi-omics data from both an in-house and a public clinical study to identify biomarkers of NAC response. We observed significant transcriptional differences associated with response in the residual disease (post-NAC biopsies), which did not exist in the pre-NAC biopsies. We further refined the post-NAC transcriptional changes to a 17-gene signature and machine learning models were applied to evaluate the signature’s diagnostic potential (area under the curve ≥ 0.8). Interestingly, the signature was enriched with down-regulated immune genes and several of these genes demonstrated prognostic potential in basal TNBC tumors. Our 17-gene signature provides additional insight into TNBC recurrence, which warrants further investigation.
A high-throughput test of anxiety for zebrafish (Danio rerio) larvae: the larval diving response (LDR)
Authors: Fontana Barbara D. , Parker Matthew O. 
Published: May 2022

AbstractBackgroundZebrafish are used in anxiety research as the species’ naturalistic diving response to a new environment is a reliable and validated marker for anxiety-like behavior. One of the benefits of using zebrafish is the potential for high throughput drug screens in fish at the larval stage. However, at present, tests of anxiety in larvae and adults often measure different endpoints.New MethodHere, for the first time, we have adapted the novel tank diving response test for examining diving behavior in zebrafish larvae to assess anxiety-like behaviors at very early-stages (7 days-post-fertilization [dpf]).Comparison with Existing MethodsCurrent methods to examine anxiety in larvae can show low reliability, and measure different endpoints as in adults, thus calling into question their translational relevance.ResultsWe found that 7dpf zebrafish spent more time at the bottom of a small novel tank. We validated this as anxiety-like behaviors with diazepam reducing, and caffeine increasing the time spent in the bottom of the novel environment.ConclusionsThis new automated and high-throughput screening tool has the potential use for screening of anxiogenic and anxiolytic compounds, and for studies aiming to understand the mechanisms underlying affective disorders.
Contact-consistent visual object pose estimation for contact-rich robotic manipulation tasks
Authors: Tian Zhonglai , Cheng Hongtai , Du Zhenjun , Jiang Zongbei , Wang Yeping 
Published: May 2022

Purpose
The purpose of this paper is to estimate the contact-consistent object poses during contact-rich manipulation tasks based only on visual sensors.

Design/methodology/approach
The method follows a four-step procedure. Initially, the raw object poses are retrieved using the available object pose estimation method and filtered using Kalman filter with nominal model; second, a group of particles are randomly generated for each pose and evaluated the corresponding object contact state using the contact simulation software. A probability guided particle averaging method is proposed to balance the accuracy and safety issues; third, the independently estimated contact states are fused in a hidden Markov model to remove the abnormal contact state observations; finally, the object poses are refined by averaging the contact state consistent particles.

Findings
The experiments are performed to evaluate the effectiveness of the proposed methods. The results show that the method can achieve smooth and accurate pose estimation results and the estimated contact states are consistent with ground truth.

Originality/value
This paper proposes a method to obtain contact-consistent poses and contact states of objects using only visual sensors. The method tries to recover the true contact state from inaccurate visual information by fusing contact simulations results and contact consistency assumptions. The method can be used to extract pose and contact information from object manipulation tasks by just observing the demonstration, which can provide a new way for the robot to learn complex manipulation tasks.
Corporate governance and risk disclosure: evidence from integrated reporting adopters
Authors: Raimo Nicola , NIcolò Giuseppe , Tartaglia Polcini Paolo , Vitolla Filippo 
Published: May 2022

Purpose
This study aims to examine the impact of corporate governance attributes, in the form of board characteristics, on risk disclosures provided through integrated reporting (IR).

Design/methodology/approach
Drawing upon an agency theory perspective, this study examines the effect of the main corporate governance board characteristics (size, gender diversity, independence and meeting frequency) on the level of risk disclosure provided by a sample of 95 IR adopters from 24 countries for 2018.

Findings
The results suggest that firms are slow to realise IR’s potential to produce innovations in risk disclosure mechanisms. In addition, certain board characteristics, such as gender diversity, independence of directors and meeting frequency, are positive drivers of the risk disclosure provided via IR.

Originality/value
To the best of the authors’ knowledge, this is the first study that investigates the impact of corporate governance mechanisms on risk disclosure provided via IR. Connecting corporate governance mechanisms to IR risk disclosure practices can contribute to enhancing the practical and theoretical understanding of the role that the board of directors may play in stimulating transparency and accountability about risks via an alternative communication tool, IR, to the benefit of both investors and other stakeholders.