bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Data-Driven Approach to Running Gait Assessment Using Inertial Measurement Units
Authors: Ross Erin , Milian Anthony , Ferlic Mason , Reed Samuel , Lepley Adam S. 
Published: September 2022

Background: Running is an extremely common exercise, both recreationally and competitively. Combined with clinical assessment, technology-driven biomechanical gait analysis can be used to examine markers of performance and injury risk in runners. Indications: The indication is to provide clinicians and sports science researchers a framework for using inertial measurement units (IMU) for data-driven, quantitative gait assessments. Technique Description: This video details practical application of IMU use in biomechanical gait assessments. Details on participant and equipment setup, in-session protocols, and selection of gait variables are included. Results: Following collection of demographic and anthropometric outcomes, IMUs should be placed on rigid segments of the lower extremity, sacrum, and trunk. In our model, we place IMUs on the foot, shank, thigh, sacrum, and lower thoracic spine. Following static anatomical calibration, running gait biomechanics are evaluated at multiple speeds using IMUs, 2-dimensional high-speed video cameras, and an instrumented treadmill. The high-speed video and IMU data are analyzed together at various parts of the gait cycle, including foot strike, mid-stance, toe-off, and flight. Many kinematic and kinetic variables (ie, unilateral discrete joint angles, joint excursions, joint moments, spatiotemporal outcomes, etc) can be selected for analysis, ideally via a collaboration between the sports science, athletic, and sports medicine teams. A collaborative approach should also be used to determine how this information will be used to alter training programs or influence injury risk in the running athlete. Discussion/Conclusion: This report details how to use a data-driven approach to evaluate running gait biomechanics using IMU technology. This framework for gait analysis is most applicable, and effective, when the team of researchers works in conjunction with coaches, sport scientists, and athletes. Utilizing this framework, training can be adapted based on the objective and clinical assessment to reduce injury risk and improve performance in the gait assessment.
Prevalence of pathological arrhythmia in patients triaged to “cardiac arrhythmia” in the emergency department: a preliminary study
Authors: Novotny Julia , Klein Matthias Michael , Haum Magda , Fichtner Stephanie Raphaela , Thienel Manuela Bernadette 
Published: September 2022

Abstract
Background
Symptoms caused by cardiac arrhythmia are common problems that lead to presentation to the emergency department. However, the prevalence of pathological heart rhythm in patients triaged for cardiac arrhythmia in the emergency department remains up to now unknown.

Methods and results
In this retrospective study, patients triaged for cardiac arrhythmia admitted to the interdisciplinary emergency department of the Ludwig-Maximilians University Hospital in Munich within 1 year were included. Subsequently, cardiac rhythm in the 12-lead electrocardiogram, clinical presentation, admission rate, and diagnosis at discharge was analyzed. A total of 558 out of 39,798 patients were triaged for cardiac arrhythmia. Of these 42.3% of patients showed a pathological heart rhythm on the initial electrocardiogram (66.9% atrial fibrillation, 16.5% atrial flutter, 16.5% others). About 80% presented in emergency severity index III (many resources are needed without critical vitals) conditions. Sixty-two percent of the pathological electrocardiogram group and 60% of the sinus rhythm group of patients were admitted to the hospital, and 34.7% with pathological electrocardiogram underwent invasive investigations (16.8% in the sinus rhythm group). In 43.4% of patients, the diagnosis of cardiac arrhythmia was already known from previous medical contacts.

Conclusion
A total of 1.8% of patients who presented to our interdisciplinary emergency department were triaged for cardiac arrhythmia. With 49.5%, the hospital admission rate was quite high but the patients presented to the emergency department in our cohort were rarely in critical condition. As a high percentage of our cohort had a history of cardiac arrhythmia, better outpatient management is needed for these patients to reduce emergency department visits and save resources.
Women in agriculture: pathways of pesticide exposure, potential health risks and vulnerability in sub-Saharan Africa
Authors: Atinkut Asmare Birtukan , Freyer Bernhard , Bingen Jim 
Published: September 2022

AbstractBackgroundPesticide exposure and its adverse impacts remain a major problem for women’s health, but a comprehensive scientific literature and summaries are still missing. With a specific focus in sub-Saharan Africa, this paper provides an overview of the scientific literature on how women deal with and are exposed to pesticides and the specific circumstances that might affect their health. We identified previously published studies from Google Scholar, Pubmed, and other electronic libraries.Main bodyIn addition to huge burden in the household, there is considerable evidence that women’s role in smallholder as well as commercial farming has become more visible and intense in sub-Saharan Africa. However, it is evident that women are a major risk group disproportionately exposed to and are affected by pesticides through multiple pathways. Closely linked to their biological characteristics, lifestyle and behavior, women experience acute-to-chronic diseases from pesticides. Women’s exposure to pesticides either during pregnancy or breastfeeding can pose a serious health risk for the fetus and the newborn babies.ConclusionOccupational as well as non-occupational exposure from pesticides seems to be grossly underestimated for women due to the perception that their work is not hazardous for their health and are not at risk of exposure. This is often the case why research on pesticide exposure and its adverse impacts for women’s health remains scarce in sub-Saharan Africa, where pesticides are often misused and poorly regulated. Therefore, further research that addresses women’s exposure to pesticides are strongly suggested.
Genome-wide detection of copy number variation in American mink using whole-genome sequencing
Authors: Davoudi Pourya , Do Duy Ngoc , Rathgeber Bruce , Colombo Stefanie M. , Sargolzaei Mehdi , Plastow Graham , Wang Zhiquan , Karimi Karim , Hu Guoyu , Valipour Shafagh , Miar Younes 
Published: September 2022

AbstractBackgroundCopy number variations (CNVs) represent a major source of genetic diversity and contribute to the phenotypic variation of economically important traits in livestock species. In this study, we report the first genome-wide CNV analysis of American mink using whole-genome sequence data from 100 individuals. The analyses were performed by three complementary software programs including CNVpytor, DELLY and Manta.ResultsA total of 164,733 CNVs (144,517 deletions and 20,216 duplications) were identified representing 5378 CNV regions (CNVR) after merging overlapping CNVs, covering 47.3 Mb (1.9%) of the mink autosomal genome. Gene Ontology and KEGG pathway enrichment analyses of 1391 genes that overlapped CNVR revealed potential role of CNVs in a wide range of biological, molecular and cellular functions, e.g., pathways related to growth (regulation of actin cytoskeleton, and cAMP signaling pathways), behavior (axon guidance, circadian entrainment, and glutamatergic synapse), lipid metabolism (phospholipid binding, sphingolipid metabolism and regulation of lipolysis in adipocytes), and immune response (Wnt signaling, Fc receptor signaling, and GTPase regulator activity pathways). Furthermore, several CNVR-harbored genes associated with fur characteristics and development (MYO5A,RAB27B,FGF12,SLC7A11,EXOC2), and immune system processes (SWAP70,FYN,ORAI1,TRPM2, andFOXO3).ConclusionsThis study presents the first genome-wide CNV map of American mink. We identified 5378 CNVR in the mink genome and investigated genes that overlapped with CNVR. The results suggest potential links with mink behaviour as well as their possible impact on fur quality and immune response. Overall, the results provide new resources for mink genome analysis, serving as a guideline for future investigations in which genomic structural variations are present.
Genome-wide association analysis of 101 accessions dissects the genetic basis of shell thickness for genetic improvement in Persian walnut (Juglans regia L.)
Authors: Wang Jiangtao , Ye Hang , Zhou Huijuan , Chen Pengpeng , Liu Hengzhao , Xi Ruimin , Wang Gang , Hou Na , Zhao Peng 
Published: September 2022

Abstract
Background
Understanding the underlying genetic mechanisms that drive phenotypic variations is essential for enhancing the efficacy of crop improvement. Persian walnut (Juglans regia L.), which is grown extensively worldwide, is an important economic tree fruit due to its horticultural, medicinal, and material value. The quality of the walnut fruit is related to the selection of traits such as thinner shells, larger filling rates, and better taste, which is very important for breeding in China. The complex quantitative fruit-related traits are influenced by a variety of physiological and environmental factors, which can vary widely between walnut genotypes.

Results
For this study, a set of 101 Persian walnut accessions were re-sequenced, which generated a total of 906.2 Gb of Illumina sequence data with an average read depth of 13.8× for each accession. We performed the genome-wide association study (GWAS) using 10.9 Mb of high-quality single-nucleotide polymorphisms (SNPs) and 10 agronomic traits to explore the underlying genetic basis of the walnut fruit. Several candidate genes are proposed to be involved in walnut characteristics, including JrPXC1, JrWAKL8, JrGAMYB, and JrFRK1. Specifically, the JrPXC1 gene was confirmed to participate in the regulation of secondary wall cellulose thickening in the walnut shell.

Conclusion
In addition to providing considerable available genetic resources for walnut trees, this study revealed the underlying genetic basis involved in important walnut agronomic traits, particularly shell thickness, as well as providing clues for the improvement of genetic breeding and domestication in other perennial economic crops.
Unexpected uterine body placenta accreta spectrum with placenta previa in a subsequent pregnancy after uterine artery embolization: a case report
Authors: Sugai Shunya , Yamawaki Kaoru , Haino Kazufumi , Nishijima Koji 
Published: September 2022

Abstract
Background
A subsequent pregnancy after uterine artery embolization (UAE) raises several concerns, one of which is placenta accreta spectrum (PAS). Placenta previa is the strongest risk factor for PAS, which is most likely to occur in the lower uterine segment. PAS without placenta previa (i.e., uterine body PAS) is considered relatively rare.

Case presentation
A 35-year-old woman, gravida 2 para 1, had undergone UAE for postpartum hemorrhage due to uterine atony after vaginal delivery in her previous pregnancy. She developed placenta previa during her subsequent pregnancy and was therefore evaluated for PAS in the lower uterine segment. On the basis of examination findings, we considered PAS to be unlikely. During cesarean section, we found that the placenta was not detached from the uterine body, and the patient was determined to have uterine body PAS. Ultimately, a hysterectomy was performed.

Conclusions
PAS can occur in a subsequent pregnancy after UAE. When a subsequent pregnancy after UAE is accompanied by placenta previa, it is important to maintain a high index of suspicion of uterine body PAS without being misled by the presence of placenta previa.
Gestational anemia and maternal antenatal and postpartum psychological distress in a prospective FinnBrain Birth Cohort Study
Authors: Kemppinen Lotta , Mattila Mirjami , Ekholm Eeva , Huolila Linda , Pelto Juho , Karlsson Hasse , Mäkikallio Kaarin , Karlsson Linnea 
Published: September 2022

AbstractBackgroundGestational anemia, most commonly caused by iron deficiency, may increase the risk of maternal anxiety and depression and have a potentially far-reaching impact on mother’s and newborn’s health. Several mechanisms, such as effects of iron deficiency on cerebral neurotransmitter metabolism, have been suggested. None of the earlier studies have assessed the association between gestational anemia and depression, anxiety and pregnancy-related anxiety simultaneously.MethodsWomen, participating in the FinnBrain Birth Cohort Study and attending maternity welfare clinics in Turku, whose hemoglobin (Hb) values during pregnancy were available were included in this study (n = 1273). The study group consisted of 301 women with Hb levels < 11.0 g/dL at any time during pregnancy, and 972 women with Hb ≥ 11.0 g/dL were included in the control group. Symptoms of depression, anxiety, and pregnancy-related anxiety were assessed using the Edinburgh Postnatal Depression Scale (EPDS), Symptom Checklist-90 (SCL), and Pregnancy-Related Anxiety Questionnaire (PRAQ) questionnaires at 14, 24, and 34 gestational weeks, and EPDS and SCL were also performed 3 and 6 months postpartum.ResultsGestational anemia was not associated with an increased risk of depression either prenatally or postpartum when the analyses were adjusted for maternal age at birth, parity, smoking during pregnancy, maternal education, and gestational age. However, a weak connection was found between gestational anemia and prenatal anxiety in the early pregnancy. Furthermore, the analysis between women with Hb < 10.0 g/dL and those with Hb ≥ 10.0 g/dL showed an association between gestational anemia and anxiety in the late pregnancy, but otherwise no difference in psychological distress was found.ConclusionsNo evidence supporting the association between gestational anemia and antenatal or postpartum depression was found. However, a weak connection between gestational anemia and antenatal anxiety was observed. This finding needs further investigation to establish timing and investigate causality.
A collaborative approach to develop an intervention to strengthen health visitors’ role in prevention of excess weight gain in children
Authors: Ray Devashish , Sniehotta Falko , McColl Elaine , Ells Louisa , O’Neill Gill , McCabe Karen 
Published: September 2022

Abstract
Background
The high prevalence of childhood obesity is a concern for public health policy and practitioners, leading to a focus on early prevention. UK health visitors (HVs) are well-positioned to prevent excessive weight gain trends in pre-school children but experience barriers to implementing guideline recommended practices. This research engaged with HVs to design an intervention to strengthen their role in prevention of early childhood obesity.

Methods
We describe the processes we used to develop a behaviour change intervention and measures to test its feasibility. We conducted a systematic review to identify factors associated with implementation of practices recommended for prevention of early childhood obesity. We carried out interactive workshops with HVs who deliver health visiting services in County Durham, England. Workshop format was informed by the behaviour change wheel framework for developing theory-based interventions and incorporated systematic review evidence. As intended recipients of the intervention, HVs provided their views of what is important and acceptable in the local context. The findings of the workshops were combined in an iterative process to inform the four steps of the Implementation Intervention development framework that was adapted as a practical guide for the development process.

Results
Theoretical analysis of the workshop findings revealed HVs’ capabilities, opportunities and motivations related to prevention of excess weight in 0-2 year olds. Intervention strategies deemed most likely to support implementation (enablement, education, training, modelling, persuasion) were combined to design an interactive training intervention. Measures to test acceptability, feasibility, and fidelity of delivery of the proposed intervention were identified.

Conclusions
An interactive training intervention has been designed, informed by theory, evidence, and expert knowledge of HVs, in an area of health promotion that is currently evolving. This research addresses an important evidence-practice gap in prevention of childhood obesity. The use of a systematic approach to the development process, identification of intervention contents and their hypothesised mechanisms of action provides an opportunity for this research to contribute to the body of literature on designing of implementation interventions using a collaborative approach. Future research should be directed to evaluate the acceptability and feasibility of the intervention.
Difference in bypass for inpatient care and its determinants between rural and urban residents in China
Authors: Lu Shan , Li Yunxiao , Gao Hongxia , Zhang Yan 
Published: September 2022

Abstract
Background
Bypass for inpatient care is an event of excessive demand. Though primary care facilities provide inpatient care in China, local residents may choose more distant higher-level hospital for inpatient care services. Given the differences in accessibility of hospitals and socioeconomic development between urban and rural areas, this study aims to estimate the rate of bypass for inpatient care and explore the factors predictive of bypass among rural and urban residents in China.

Methods
The rates of bypass for inpatient care were estimated using data from 1352 hospitalized patients, obtained from the 2018 Sixth National Health Service Survey of Hubei, China. Bypass for inpatient care was identified if the patient was hospitalized in a hospital for a certain disease that should be treated at primary care facilities in accordance with government requirement. Anderson’s Behavioral Model of Health Services Use was used as a theoretical framework for determining the factors of bypass. Logistic regression was used to identify the relationship between bypass for inpatient care and predisposing, enabling, and need characteristics for urban and rural residents.

Results
The rate of bypass for inpatient care was 73.8%. This rate for inpatient care (91.3%) in cities is higher than that in rural areas (56.2%). Age were associated with bypass for both rural (OR, 0.982; 95% CI, 0.969–0.995) and urban (OR, 0.947; 95% CI, 0.919–0.976) patients. The patients whose closest healthcare facility was hospitals were more likely to have bypass behavior in rural (OR, 26.091; 95% CI, 7.867–86.537) and urban (OR, 8.323; 95% CI, 2.936–23.591) areas than those living closest to township/community health centers. Signing a family doctor was not helpful for retaining patients at primary care facility. Among rural patients, those with circulatory (OR, 2.378; 95% CI, 1.328–4.258), digestive (OR, 2.317; 95% CI, 1.280–4.192), or skin and bone (OR, 1.758; 95% CI, 1.088–2.840) system diseases were more likely to show bypass behavior than those with respiratory diseases.

Conclusions
Bypass for inpatient care is sizable, and urban residents have a higher bypass rate for inpatient care than rural residents in China. More actionable measures in strengthening and leading patients to primary care are needed. Gradual establishment of a referral system is recommended. Inpatient care for circulatory, digestive, or skin and bone system diseases may be prioritized to be improved at primary care facilities in rural China.
Detection and discovery of plant viruses in soybean by metagenomic sequencing
Authors: Elmore Manjula G. , Groves Carol L. , Hajimorad M. R. , Stewart Tracey P. , Gaskill Mikaela A. , Wise Kiersten A. , Sikora Edward , Kleczewski Nathan M. , Smith Damon L. , Mueller Daren S. , Whitham Steven A. 
Published: September 2022

AbstractBackgroundViruses negatively impact soybean production by causing diseases that affect yield and seed quality. Newly emerging or re-emerging viruses can also threaten soybean production because current control measures may not be effective against them. Furthermore, detection and characterization of new plant viruses requires major efforts when no sequence or antibody-based resources are available.MethodsIn this study, soybean fields were scouted for virus-like disease symptoms during the 2016–2019 growing seasons. Total RNA was extracted from symptomatic soybean parts, cDNA libraries were prepared, and RNA sequencing was performed using high-throughput sequencing (HTS). A custom bioinformatic workflow was used to identify and assemble known and unknown virus genomes.ResultsSeveral viruses were identified in single or mixed infections. Full- or nearly full-length genomes were generated for tobacco streak virus (TSV), alfalfa mosaic virus (AMV), tobacco ringspot virus (TRSV), soybean dwarf virus (SbDV), bean pod mottle virus (BPMV), soybean vein necrosis virus (SVNV), clover yellow vein virus (ClYVV), and a novel virus named soybean ilarvirus 1 (SIlV1). Two distinct ClYVV isolates were recovered, and their biological properties were investigated inNicotiana benthamiana, broad bean, and soybean. In addition to infections by individual viruses, we also found that mixed viral infections in various combinations were quite common.ConclusionsTaken together, the results of this study showed that HTS-based technology is a valuable diagnostic tool for the identification of several viruses in field-grown soybean and can provide rapid information about expected viruses as well as viruses that were previously not detected in soybean.
Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas
Authors: Wang Depeng , Gong Jie , Zhang Hui , Liu Yulu , Sun Nannan , Hao Xiaomeng , Mu Kun 
Published: September 2022

Abstract
Objectives
To investigate lymphoid enhancer factor 1 (LEF-1) protein expression in medulloblastomas (MBs) and its correlation with molecular grouping of MBs.

Methods
Expressions of LEF-1 and β-catenin were detected by immunohistochemistry, and molecular grouping was performed based on the NanoString and sequencing techniques for 30 MBs.

Results
By genetic defining, 3 MBs were WNT-activated, 11 were SHH-activated, 3 were in Group 3 and 13 in Group 4 respectively. Nuclear LEF-1 staining was found in 8 MBs using immunohistochemical method. Three out of 8 showed diffuse and strong nuclear LEF-1 staining which were proved to be WNT-activated genetically, while the other 5 MBs with focal staining were SHH-activated genetically. The expression of LEF-1 protein was significantly correlated with genetically defined WNT-activated MBs (P < 0.0001). We also found focal nuclear β-catenin expression ( less than 1% of tumor cells) in 5 MBs. LEF-1 positivity was significantly correlated nuclear β-catenin expression (p < 0.001).

Conclusions
Immunohistochemical staining of LEF-1 can be used as a supplement for β-catenin to diagnosis WNT-activated Medulloblastomas, when β-catenin is difficult to recognize for its cytoplasm/membrane staining background. Diffuse nuclear staining of LEF-1 indicates WNT-activated MB.