bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic profiling of single circulating tumor cells provides insight into human metastatic gastric cancer
Authors: Negishi Ryo , Yamakawa Hitomi , Kobayashi Takeru , Horikawa Mayuko , Shimoyama Tatsu , Koizumi Fumiaki , Sawada Takeshi , Oboki Keisuke , Omuro Yasushi , Funasaka Chikako , Kageyama Akihiko , Kanemasa Yusuke , Tanaka Tsuyoshi , Matsunaga Tadashi , Yoshino Tomoko 
Published: January 2022

AbstractTranscriptome analysis of circulating tumor cells (CTCs), which migrate into blood vessels from primary tumor tissues, at the single-cell level offers critical insights into the biology of metastasis and contributes to drug discovery. However, transcriptome analysis of single CTCs has only been reported for a limited number of cancer types, such as multiple myeloma, breast, hepatocellular, and prostate cancer. Herein, we report the transcriptome analysis of gastric cancer single-CTCs. We utilized an antigen-independent strategy for CTC isolation from metastatic gastric cancer patients involving a size-dependent recovery of CTCs and a single cell isolation technique. The transcriptomic profile of single-CTCs revealed that a majority of gastric CTCs had undergone epithelial-mesenchymal transition (EMT), and indicated the contribution of platelet adhesion toward EMT progression and acquisition of chemoresistance. Taken together, this study serves to employ CTC characterization to elucidate the mechanisms of chemoresistance and metastasis in gastric cancer.
Is Bilingual Receptive Vocabulary Assessment via Telepractice Comparable to Face-to-Face?
Authors: Castilla-Earls Anny , Ronderos Juliana , McIlraith Autumn , Martinez Damaris 
Published: January 2022

Purpose:
This study investigated the effect of delivery method (face-to-face or telepractice), time, home language, and language ability on bilingual children's receptive vocabulary scores in Spanish and English.

Method:

Participants included bilingual children with (
n
= 32) and without (
n
= 57) developmental language disorders (DLD) that were assessed at 2 time points about 1 year apart. All children participated in face-to-face assessment at Time 1. At Time 2, 41 children were assessed face-to-face and 48 children were assessed using telepractice.

Results:
Delivery method was not a significant predictor of receptive scores in either Spanish or English. Spanish and English receptive vocabulary increased over time in both children with and without DLD. Children with DLD had lower receptive vocabulary raw scores than children with typical development. Children who spoke English-only at home had significantly higher English receptive scores than children who spoke Spanish-only or both Spanish and English at home.

Conclusions:
Face-to-face and telepractice assessments seem to be comparable methods for the assessments of Spanish and English receptive skills. Spanish and English receptive skills increased over time in children with and without DLD.

Supplemental Material:

https://doi.org/10.23641/asha.17912297
Motor activity of nursing students in the conditions of the COVID-19 pandemic
Authors: Popova Nikoleta , Georgieva Anna , Digalovska Vasilena 
Published: January 2022

The COVID-19 pandemic has changed all areas of human life and activity. A number of lifestyle changes associated with health risk factors have occurred, one of which is inactivity. The purpose of this study is to investigate the opinion of nursing students about their motor activity in the conditions of the COVID-19 pandemic. Material and methods: In our report, we have applied analysis of literary sources and a survey (on-line anonymous group survey). The opinion of 60 nursing students of second, third and fourth year at the Medical University – Varna was studied. The nurses received explanation for the purpose and methodology of the study, their anonymity was guaranteed, and their informed consent for the study was obtained. The study was conducted in August 2020–January 2021. Graphical analysis was used to visualize the observed processes and phenomena. To create the graphs, we used Microsoft Office Excel 2013. The data were processed using the IBM SPSS Statistics 19 statistical package. Results: The majority of the respondents shared that they had been less active compared to the time before the pandemic began. The search for alternatives for exercising and sports among students is hampered by a number of factors, which relate to the changes in the organization of life and educational activities associated with measures to stop the pandemic.
Conclusion: The results of this study prove that the COVID-19 pandemic has had an impact on the lifestyle of the nursing students - most of them have reduced their motor activity. The data highlights the need to find alternatives and strategies to reduce the inactivity.
Arthroskopisch assistierte Versorgung einer intraartikulären Skapulafraktur bei einem Rugbyspieler
Authors: Szewczyk Karol , Mann Jakob , Ellwein Alexander , Jensen Gunnar , Warnhoff Mara , Hahner Florian , Lill Helmut , Dey Hazra Rony-Orijit 
Published: January 2022

ZusammenfassungBei Skapulafrakturen handelt es sich um eine seltene Verletzungsentität mit einer Inzidenz von 0,4–1 % aller Frakturen und 3–5 % der Frakturen des Schultergürtels. Im vorliegenden Fall handelt es sich um einen 29-jährigen männlichen Patienten, der im Rahmen eines Punktspiels der Rugby-Bundesliga die o. g. Verletzung nach einem direkten Anpralltrauma erlitt. Nach der klinischen sowie radiologischen Untersuchung zeigten sich eine mehrfragmentäre, dislozierte Skapulablattfraktur unter mehrfragmentärer Beteiligung der Margo superior, Frakturausläufer in die Margo medialis und V. a. Stufenbildung im Glenoid bei äquatorialer Transversalfraktur (Euler und Rüedi D2b/nach AO 14 F1.3e). Die operative Versorgung erfolgte in minimalinvasiver arthroskopischer Technik, und postoperativ wurde eine frühfunktionelle Nachbehandlung ohne Belastung und mit einer Bewegungslimitierung von 90° Abduktion/Anteversion durchgeführt. Bereits am 1. Spieltag der Rückrunde (5 Monate postoperativ) war der Patient wieder vollständig einsatzfähig und 6 Monate postoperativ präsentierte sich der Patient beschwerdefrei mit einem exzellenten klinischen Resultat (CS 100 Punkte, SSV 98 %, OSS 12 Punkte, ASES-Score 100 Punkte). Zusammenfassend ist die arthroskopisch assistierte Versorgung einer intraartikulären Skapulafraktur beim sportlich aktiven Patienten mit einem entsprechend hohen körperlichen Anspruch eine suffiziente Therapieoption.
History of Evolution of Posterolateral Approaches to the Thoracic Spine: From Cure of Pott's Disease to Epidural Tumor Resection
Authors: Gagliardi Filippo , Pompeo Edoardo , De Domenico Pierfrancesco , Snider Silvia , Roncelli Francesca , Acerno Stefania , Mortini Pietro 
Published: January 2022

AbstractSince the end of the nineteenth century, the wide dissemination of Pott's disease has ignited debates about which should be the ideal route to perform ventrolateral decompression of the dorsal spine in case of paraplegia due to spinal cord compression in tuberculosis spondylitis. It was immediately clear that the optimal approach should be the one minimizing the surgical manipulation on both neural and extraneural structures while optimizing the exposure and surgical maneuverability on the target area. The first attempt was reported by Victor Auguste Menard in 1894, who described, for the first time, a completely different route from traditional laminectomy, called costotransversectomy. The technique was conceived to drain tubercular paravertebral abscesses causing paraplegia without manipulating the spinal cord. Over the following decades, many other routes have been described all over the world, thus demonstrating the wide interest on the topic. Surgical development has been marked by the new technical achievements and by instrumental/technological advancements, until the advent of portal surgery and endoscopy-assisted techniques. In this article, the authors retraced the milestones of this history up to the present days, through a systematic review on the topic.
Biaxial Flexural Strength of Different Monolithic Zirconia upon Post-Sintering Processes
Authors: Juntavee Niwut , Juntavee Apa , Phattharasophachai Thipradi 
Published: January 2022

AbstractObjective Different post-sintering processes are expected to be a reason for alteration in the strength of zirconia. This study evaluated the effect of post-sintering processes on the flexural strength of different types of monolithic zirconia.Materials and Methods A total of 120 classical- (Cz) and high-translucent (Hz) monolithic zirconia discs (1.2 mm thickness and 14 mm in Ø) were prepared, sintered, and randomly divided into four groups to be surface-treated with (1) as-glazed (AG); (2) finished and polished (FP); (3) finished, polished, and overglazed (FPOG); and (4) finished, polished, and heat-treated (FPHT) technique (n = 15). Biaxial flexural strength (σ) was determined on a piston-on-three ball in a universal testing machine at a speed of 0.5 mm/min.Statistical Analysis Analysis of variance, and post hoc Bonferroni multiple comparisons were determined for significant differences (α = 0.05). Weibull analysis was applied for survival probability, Weibull modulus (m), and characteristic strength (σ0). The microstructures were examined with a scanning electron microscope and X-ray diffraction.Results The mean ± standard deviation value of σ (MPa), m, and σ0 were 1,626.43 ± 184.38, 9.51, and 1,709.79 for CzAG; 1,734.98 ± 136.15, 12.83, and 1,799.17 for CzFP; 1,636.92 ± 130.11, 14.66, and 1,697.63 for CzFPOG; and 1,590.78 ± 161.74, 10.13, and 1,663.82 for CzFPHT; 643.30 ± 118.59, 5.59, and 695.55 for HzAG; 671.52 ± 96.77, 3.28, and 782.61 for HzFP; 556.33 ± 122.85, 4.76, and 607.01 for HzFPOG; and 598.36 ± 57.96, 11.22, and 624.89 for HzFPHT. The σ was significantly affected by the post-sintering process and type of zirconia (p < 0.05), but not by their interactions (p > 0.05). The Cz indicated a significantly higher σ than Hz. The FP process significantly enhanced σ more than other treatment procedures.Conclusion Post-sintering processes enabled an alteration in σ of zirconia. FP enhanced σ, while FPOG and FPHT resulted in a reduction of σ. Glazing tends to induce defects at the glazing interface, while heat treatment induces a phase change to tetragonal, both resulted in reducing σ. Finishing and polishing for both Cz and Hz monolithic zirconia is recommended, while overglazed or heat-treated is not suggested.
Relationship of Malocclusions with Disorders of the Temporomandibular Joint in Children of CALI—Colombia
Authors: Mora-Zuluaga Nataly , Soto-Llanos Libia , Aragón Natalia , Torres-Trujillo Katherine 
Published: January 2022

AbstractObjective The aim of this study was to determine the relationship of malocclusion with the presence and severity of temporomandibular disorders (TMDs) in children.Materials and Methods A clinical examination was performed in 87 patients (from 4 to 14 years of age) who attended the dentistry clinics of Universidad del Valle.Results The 77 patients studied had malocclusions; 55 patients had TMD and 67.3% were female. The most frequent symptom of TMD was articular unilateral noise with 33.8%, followed by pain in at least one masticatory muscle with 26%. TMJ pain was observed in 24.7% of the patients. There was a statistically significant relationship between the presence and severity of TMD with type of dentition and transverse malocclusion, respectively.Conclusion The presence of TMD in children with malocclusion presented in a high frequency. TMD depends on the type of dentition and its severity is dependent on transverse malocclusion.
The Effectiveness of 0.2% Chlorhexidine Gel on Early Wound Healing after Tooth Extraction: A Randomized Controlled Trial
Authors: Amaliya Amaliya , Ramadhanti Rika , Hadikrishna Indra , Maulina Tantry 
Published: January 2022

Abstract
Objective This study aimed to evaluate the effect of 0.2% chlorhexidine (CHX) gel on wound healing after tooth extraction.
Materials and Methods A single blind, randomized controlled trial was performed recruiting 32 participants who underwent dental extractions. Patients were randomly allocated for CHX group or placebo group. The primary outcomes were wound closure measured with calipers and healings were assessed by Landry et al index after 7 days of topical application of allocated gels on extraction sites.
Results The wound closures were greater in CHX group compared with placebo group and healing scores were correlated with the use of CHX gel (p-value < 0.05).
Conclusion In a population of healthy nonsmoker adults, application of 0.2% CHX gel twice a day for 7 days after tooth extraction has a beneficial effect on wound healing.
Percutaneous Cryoablation of Renal Tumors: Initial Indian Experience
Authors: Gorsi Ujjwal , Jain Rishabh , Bansal Akash , Kalra Naveen , Kang Mandeep , Chaluvashetty Sreedhara B. , Mavuduru Ravimohan Suryanarayan , Kumar Santosh , Singh Shrawan K. , Sandhu Manavjit Singh 
Published: January 2022

Abstract
Purpose The purpose of this study was to report short-term outcomes of cryoablation of early-stage renal tumors (T1a and T1b) at a tertiary hospital in India.
Methods This was a retrospective study of consecutive patients who underwent cryoablation for renal cell carcinoma (RCC) from November 2018 to June 2020. Cryoablation was performed under combined ultrasound and computed tomography guidance using a helium-argon cryoablation system. Tumor number, size, location, nephrometry score, relationship of the tumor with pelvicalyceal system, and adjacent organs were tabulated, and technical and clinical success evaluated. Early and late recurrence and complications were also assessed.
Results Eleven patients (median age: 62 years) with 11 tumors underwent cryoablation. The mean tumor size was 2.58 cm (range: 1.62–5.62 cm) with 10 lesions being T1a and one lesion T1b. Tissue sampling was done in 9/11 patients, 3 were papillary RCC and the rest, clear cell RCC. In two patients, the tumor was completely endophytic, three patients had partially endophytic tumors while 6 patients had exophytic lesions. The median nephrometry score was 6 (range: 4–11, Mode 4). Technical success was achieved in all patients. Complete response was achieved in 81% (9/11) of the patients at 1-month follow-up. Median follow-up period was 6 months. Two patients showed residual disease on follow-up imaging at 1 and 3 months, respectively.
Conclusion Cryoablation is a promising, relatively new minimally invasive therapy for treating small renal tumors in India. It is safe, technically feasible, and shows excellent short-term efficacy.
A simulation approach to predict the calibration curve of low LET radiations for biological dosimetry using the Geant4-DNA toolkit
Authors: Mirrezaei E. , Setayeshi S. , Zakeri F. , Baradaran S. 
Published: January 2022

Abstract
Ionizing radiation is extensively utilized in various applications; however, it can lead to significant harm to living systems. In this regard, the radiation absorbed dose is usually evaluated by performing biological dosimetry and physical reconstruction of exposure scenarios. But, this is costly, time-consuming, and maybe impractical for a biodosimetry lab to perform biological dosimetry. This study aimed to assess the applicability and reliability of the Geant4-DNA toolkit as a simulation approach to construct a reliable dose-response curve for biodosimetry purposes as an appropriate substitution for experimental measurements. In this matter, the total number of double-strand breaks (DSBs), due to different doses of low LET radiation qualities on DNA molecules, was calculated and converted to the values of dicentric chromosomes using a mechanistic model of cellular response. Then, the number of dicentric chromosomes induced by 200 kVp X-rays were modified by using a semi-empirical scaling factor for compensating the restriction of simulation code to consider what can happen in a real cell. Next, the trend of dicentrics for 137Cs and 60Co were calculated and modified by the above scaling factor. Finally, the dose-response curves for these gamma sources compared to several published experiments. The suggested calibration curves for

137Cs and 60Co
followed a linear quadratic equation: Ydic = 0.0054 (± 0.0133) - 0.0089 (± 0.0212) × D + 0.0568 (± 0.0051) × D2 and Ydic = 0.0052 (± 0.0128) - 0.00568 (± 0.0203) × D + 0.0525 (± 0.0049) × D2 respectively. They revealed a satisfactory agreement with the experimental data reported by others. The Geant4 program developed in this work could provide an appropriate tool for predicting the dose-response (calibration) curve for biodosimetry purposes.
Subdiffusive search with home returns via stochastic resetting: a subordination scheme approach
Authors: Stanislavsky Aleksander A , Weron Aleksander 
Published: January 2022

Abstract
Stochastic resetting with home returns is widely found in various manifestations in life and nature. Using the solution to the home return problem in terms of the solution to the corresponding problem without home returns (Pal et al 2020 Phys. Rev. Res.
2 043174), we develop a theoretical framework for search with home returns in the case of subdiffusion. This makes a realistic description of restart by accounting for random walks with random stops. The model considers stochastic processes, arising from Brownian motion subordinated by an inverse infinitely divisible process (subordinator).
Moiré patterns and carbon nanotube sorting
Authors: Konevtsova Olga V , Roshal Daria S , Rochal Sergei B 
Published: January 2022

Abstract
Moiré patterns (MPs), arising from the superposition of two lattices with close periods, are tightly related to the physicochemical properties of bilayer nanostructures. Here, we develop the theory of complex MPs emerging in twisted bilayer graphene and planar nets of double-walled nanotubes at significant relative twist and/or deformation of layers. The proposed theory clarifies the physicochemical regularities arising at sorting of single-walled carbon nanotubes (SWCNTs) by organic molecules, which self-assemble in regular coatings on both the tubes and planar graphene. We introduce and consider an outer tubular virtual lattice that is a parent structure for the deposited coating and due to this fact, its existence is crucial for the coating formation. As we show, such outer lattices exist only for successfully sorted SWCNTs and the superposition between the outer lattice and SWCNT forms a specific long-period MP. We explain known experimental results of SWCNT sorting by molecules of flavin group, poly(9,9-dioctylfluorene-2,7-diyl), and poly [(m-phenylenevinylene)-alt-(p-phenylenevinylene)]. Also, our approach points out other organic molecules and polymers suitable for effective carbon nanotube sorting.
Influence of tool rotational speed on the evolution of microstructure and mechanical properties of precipitation-hardened Aluminium 6061 butt joint during friction stir welding
Authors: Ghosh Biplab , Das Hrishikesh , Samanta Asis , Majumdar Jyotsna Dutta , Ghosh Manojit 
Published: January 2022

Abstract
The present investigation intends to interpret the effect of tool rotational speed on the mechanical properties and microstructural evolution in Aluminium 6061-T6 alloy during friction stir welding. A higher value of tool rotation produces more hardness at the nugget zone, which is attributed to the higher intensity of reprecipitation at higher rpm, revealed by transmission electron microscopy. The nugget zone is revealed as a nearly precipitate-free region, while the thermo-mechanically affected zone contains coarse precipitates, deformed and dynamically recovered grains with a few recrystallized grains. Significant reduction in grain size in the stirred zone is also a key finding. The observations depict the dependence of microstructure, and thus mechanical behaviour on tool rotational speed. A specific combination of process parameters has been determined from experiments, which corresponds to the maximum joint efficiency.
RdRp-based sensitive taxonomic classification of RNA viruses for metagenomic data
Authors: Tang Xubo , Shang Jiayu , Sun Yanni 
Published: January 2022

Abstract
With advances in library construction protocols and next-generation sequencing technologies, viral metagenomic sequencing has become the major source for novel virus discovery. Conducting taxonomic classification for metagenomic data is an important means to characterize the viral composition in the underlying samples. However, RNA viruses are abundant and highly diverse, jeopardizing the sensitivity of comparison-based classification methods. To improve the sensitivity of read-level taxonomic classification, we developed an RNA-dependent RNA polymerase (RdRp) gene-based read classification tool RdRpBin. It combines alignment-based strategy with machine learning models in order to fully exploit the sequence properties of RdRp. We tested our method and compared its performance with the state-of-the-art tools on the simulated and real sequencing data. RdRpBin competes favorably with all. In particular, when the query RNA viruses share low sequence similarity with the known viruses ($\sim 0.4$), our tool can still maintain a higher F-score than the state-of-the-art tools. The experimental results on real data also showed that RdRpBin can classify more RNA viral reads with a relatively low false-positive rate. Thus, RdRpBin can be utilized to classify novel and diverged RNA viruses.
Feasibility of discharge within 72 hours of major colorectal surgery: lessons learned after 5 years of institutional experience with the ERAS protocol
Authors: Biondi Alberto , Mele Maria Cristina , Agnes Annamaria , Lorenzon Laura , Cintoni Marco , Rinninella Emanuele , Marincola Giuseppe , D’Ugo Domenico , Gasbarrini Antonio , Persiani Roberto 
Published: January 2022

Abstract

Background
Early postoperative discharge after colorectal surgery within the enhanced recovery after surgery (ERAS) guidelines has been demonstrated to be safe, although its applicability has not been universal. The primary aim of this study was to identify the predictors of early discharge and readiness for discharge in a study population.

Methods
Early discharge was defined as discharge occurring in 72 h or less after surgery. The characteristics and clinical outcomes of the patients in the early and non-early discharge groups were compared, and variables associated with early discharge were identified. Additionally, independent variables associated with the readiness for discharge within 48 h were evaluated.

Results
Of 965 patients who underwent colorectal surgery between January 2015 and July 2020, 788 were included in this study. No differences in readmission, reoperation, or 30-day mortality were observed between the early and non-early discharge groups. Both early discharge and readiness for discharge had a positive association with adherence to 80 per cent or more of the ERAS items and a negative association with the female sex, duration of surgery, drain positioning, and postoperative complications.

Conclusion
Early discharge after colorectal surgery is safe and feasible, and is not associated with a high risk of readmission or reoperation. Discharge at 48 h can be reliably predicted in a subset of patients. Future studies should collect prospective data on early discharge related to safety, as well as patients’ expectations, possible organizational issues, and effective costs reduction in Italian clinical practice.