bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robust linear DNA degradation supports replication–initiation-defective mutants in Escherichia coli
Authors: Rao T V Pritha , Kuzminov Andrei 
Published: September 2022

Abstract
RecBCD helicase/nuclease supports replication fork progress via recombinational repair or linear DNA degradation, explaining recBC mutant synthetic lethality with replication elongation defects. Since replication initiation defects leave chromosomes without replication forks, these should be insensitive to the recBCD status. Surprisingly, we found that both Escherichia coli dnaA46(Ts) and dnaC2(Ts) initiation mutants at semi-permissive temperatures are also recBC-colethal. Interestingly, dnaA46 recBC lethality suppressors suggest underinitiation as the problem, while dnaC2 recBC suppressors signal overintiation. Using genetic and physical approaches, we studied the dnaA46 recBC synthetic lethality, for the possibility that RecBCD participates in replication initiation. Overproduced DnaA46 mutant protein interferes with growth of dnaA+ cells, while the residual viability of the dnaA46 recBC mutant depends on the auxiliary replicative helicase Rep, suggesting replication fork inhibition by the DnaA46 mutant protein. The dnaA46 mutant depends on linear DNA degradation by RecBCD, rather than on recombinational repair. At the same time, the dnaA46 defect also interacts with Holliday junction-moving defects, suggesting reversal of inhibited forks. However, in contrast to all known recBC-colethals, which fragment their chromosomes, the dnaA46 recBC mutant develops no chromosome fragmentation, indicating that its inhibited replication forks are stable. Physical measurements confirm replication inhibition in the dnaA46 mutant shifted to semi-permissive temperatures, both at the level of elongation and initiation, while RecBCD gradually restores elongation and then initiation. We propose that RecBCD-catalyzed resetting of inhibited replication forks allows replication to displace the “sticky” DnaA46(Ts) protein from the chromosomal DNA, mustering enough DnaA for new initiations.
Thermal conductivity at elevated temperature, density and geochemical signatures for the massive rhyolites of the Bundelkhand Craton, central India
Authors: Ray Labani , Chopra Nishu , Singh S P , Hiloidari Sikha , Rao S Eswara 
Published: September 2022

SUMMARY
In spite of the fact that rhyolite constitute a vital part of the key tectonic environments, such as continental rift-arc systems and oceanic islands, the data on the thermal and physical properties are scarce, which hinders the exact thermal modelling of these regions. Here, we have investigated the thermal conductivity from room temperature (25 °C) to elevated temperatures (up to 300 °C) for 11 massive rhyolite samples, collected from the greenstone belt of the Bundelkhand Craton, central India. The petrographical, geochemical (major oxide and trace elements) and physical (density and porosity) properties have been studied to characterize the samples before measurement of thermal conductivity at elevated temperatures. Geochemical results indicate that these rhyolites are high-K (K2O: 3.6–5.4 wt. per cent), calc-alkaline in nature with enriched REE signatures {(La/Yb)N: 9.4–22.3, (Gd/Yb)N: 1.2–1.9} and are similar to FI-type Archaean rhyolites. The density of these rhyolites depicts a narrow range between 2590 and 2690 kg m−3, with an average of 2637 kg m−3 and negligible porosity. Their thermal conductivity at room temperature varies between 2.5 and 3.3 W m−1 K−1, with an average of 2.8 W m−1 K−1; the decrease in thermal conductivity from room temperature to 300 °C ranges between 16 and 32 per cent, with an average of 23 per cent; and the temperature coefficient of thermal conductivity b, in the expression λT = λRT (1 + bT)−1, varies between 0.7 × 10−3 and 1.7 × 10−3 K−1 with an average of 1.1 × 10−3 K−1. Our study reveals that the massive rhyolites have an almost similar density as their intrusive equivalent like Bundelkhand granitoids, but their thermal properties, such as thermal conductivity at room temperature (λRT), decrease in thermal conductivity with temperatures (Δλ) and the temperature coefficient of thermal conductivity (b), lies between the two extreme variety of the granitoids, that is (i) alkali feldspar granite to monzogranite and (ii) granodiorite to tonalite to quartz diorite. We suggest that the temperature coefficient of the massive rhyolite can be expressed as b = 0.81 × λRT–1.21, which will be useful in determining the thermal conductivity of such rhyolites at elevated temperatures from their thermal conductivity at room temperature (λRT). Thermal and physical parameters reported for rhyolites will provide important constraints in various geophysical and thermo-mechanical modelling for the rhyolitic terrains.
The First Reconstruction of the Head Anatomy of a Cretaceous Insect, †Gerontoformica gracilis(Hymenoptera: Formicidae), and the Early Evolution of Ants
Authors: Richter Adrian , Boudinot Brendon , Yamamoto Shûhei , Katzke Julian , Beutel Rolf Georg 
Published: September 2022

AbstractThe fossil record allows a unique glimpse into the evolutionary history of organisms living on Earth today. We discovered a specimen of the stem group ant †Gerontoformica gracilis (Barden and Grimaldi, 2014) in Kachin amber with near-complete preservation of internal head structures, which we document employing µ-computed-tomography-based 3D reconstructions. We compare †Gerontoformica to four outgroup taxa and four extant ant species, employing parsimony and Bayesian ancestral state reconstruction to identify morphological differences and similarities between stem and crown ants and thus improve our understanding of ant evolution through the lens of head anatomy. Of 149 morphological characters, 87 are new in this study, and almost all applicable to the fossil. †Gerontoformica gracilis shares shortened dorsal tentorial arms, basally angled pedicels, and the pharyngeal gland as apomorphies with other total clade Formicidae. Retained plesiomorphies include mandible shape and features of the prepharynx. Implications of the reconstructed transitions especially for the ant groundplan are critically discussed based on our restricted taxon sampling, emphasizing the crucial information derived from internal anatomy which is applied to deep time for the first time. Based on the falcate mandible in †Gerontoformica and other Aculeata, we present hypotheses for how the shovel-shaped mandibles in crown Formicidae could have evolved. Our results support the notion of †Gerontoformica as ‘generalized’ above-ground predator missing crucial novelties of crown ants which may have helped the latter survive the end-Cretaceous extinction. Our study is an important step for anatomical research on Cretaceous insects and a glimpse into the early evolution of ant heads.
LC–MS-MS Determination of Cytostatic Drugs on Surfaces and in Urine to Assess Occupational Exposure
Authors: Lema-Atán José Ángel , Lendoiro Elena , Paniagua-González Lucía , Cruz Angelines , López-Rivadulla Manuel , de-Castro-Ríos Ana 
Published: September 2022

Abstract
The ever-increased usage of cytostatic drugs leads to high risk of exposure among healthcare workers. Moreover, workers are exposed to multiple compounds throughout their lives, leading to cumulative and chronic exposure. Therefore, multianalyte methods are the most suitable for exposure assessment, which minimizes the risks from handling cytostatic drugs and ensures adequate contamination containment. This study describes the development and full validation of two liquid chromatography–tandem mass spectrometry methods for the detection of gemcitabine, dacarbazine, methotrexate, irinotecan, cyclophosphamide, doxorubicinol, doxorubicin, epirubicin, etoposide, vinorelbine, docetaxel and paclitaxel in working surfaces and urine samples. The urine method is the first to measure vinorelbine and doxorubicinol. For surfaces, limits of detection (LOD) and limits of quantification (LOQ) were 5–100 pg/cm2, and linearity was achieved up to 500 pg/cm2. Inaccuracy was between −11.0 and 8.4%. Intra-day, inter-day and total imprecision were &lt;20%, except for etoposide and irinotecan (&lt;22.1%). In urine, LOD and LOQ were 5–250 pg/mL, with a linear range up to 1,000–5,000 pg/mL. Inaccuracy was between −3.8 and 14.9%. Imprecision was &lt;12.4%. Matrix effect was from −58.3 to 1,268.9% and from −66.7 to 1,636% in surface and urine samples, respectively, and extraction efficiency from 10.8 to 75% and 47.1 to 130.4%, respectively. All the analytes showed autosampler (6°C/72 h), freezer (–22°C/2 months) and freeze/thaw (three cycles) stability. The feasibility of the methods was demonstrated by analyzing real working surfaces and patients’ urine samples. Contamination with gemcitabine, irinotecan, cyclophosphamide, epirubicin and paclitaxel (5–4,641.9 pg/cm2) was found on biological safety cabinets and outpatients’ bathrooms. Analysis of urine from patients under chemotherapy identified the infused drugs at concentrations higher than the upper LOQ. These validated methods will allow a comprehensive evaluation of both environmental and biological contamination in hospital settings and healthcare workers.
Pediatric Phaeohyphomycosis: A 44-Year Systematic Review of Reported Cases
Authors: Castillo Bejarano José Iván , de los Santos Abiel Mascareñas , Saldaña Daniela Cisneros , Zambrano Lucio Miguel , Pérez Cavazos Samantha , Espinosa Villaseñor Fernando , de la O Cavazos Manuel Enrique , Vaquera Aparicio Denisse Natalie 
Published: September 2022

Abstract

Objective
Phaeohyphomycosis is an infection caused by pigmented fungi, which can be life-threatening in immunocompromised hosts and in disseminated disease. In adults with disseminated disease mortality is as high as 79%. Data in children is derived from case reports and series. We conducted this study to review the characteristics of phaeohyphomycoses in children.

Methods
We conducted this study following the PRISMA 2020 guideline for reporting systematic reviews. We performed a review of the reported cases of pediatric phaeohyphomycoses in core bibliographic databases published in the English and Spanish-language, between June 1977 and October 2021. We included all eligible cases in patients &lt;18 years to determine the clinical characteristics, diagnosis, treatment, and outcomes.

Results
130 cases were reviewed. Mean age was 8 years. The most common underlying conditions and risk factors included hematologic malignancies (32.5%), neutropenia (26.9%), steroid therapy (24.6%), trauma or surgery (23.1%), and children that received a transplant (14.6%). The most common presentation was localized infection (61.5%); skin and soft tissue infections were the most prevalent (25.4%). Exserohilum spp (20.8%) and Exophiala spp (17.7%) were the most common organisms isolated. Antifungal therapy remains as the most frequent treatment (87%). Overall mortality rate was 22.3% (localized 13.7% vs disseminated 37.3%).

Conclusion
The findings of this review suggest that phaeohyphomycoses in children has a better outcome compared to adults. We report a lower mortality rate in children when compared with adults in disseminated infection (37.3% vs 79%) and CNS infection (50% vs 60-70%). However, there is a wide variation in mortality rates according to infection site, treatment, and underlying conditions. Prospective studies are needed.
Interpolating between the Jaccard distance and an analogue of the normalized information distance
Authors: Kjos-Hanssen Bjørn 
Published: September 2022

Abstract
Jiménez, Becerra and Gelbukh (2013) defined a family of ‘symmetric Tversky ratio models’ $S_{\alpha ,\beta }$, $0\le \alpha \le 1$, $\beta&gt;0$. Each function $D_{\alpha ,\beta }=1-S_{\alpha ,\beta }$ is a semimetric on the powerset of a given finite set.
We show that $D_{\alpha ,\beta }$ is a metric if and only if $0\le \alpha \le \frac 12$ and $\beta \ge 1/(1-\alpha )$. This result is formally verified in the Lean proof assistant.
The extreme points of this parametrized space of metrics are $\mathcal V_1=D_{1/2,2}$, the Jaccard distance and $\mathcal V_{\infty }=D_{0,1}$, an analogue of the normalized information distance of M. Li, Chen, X. Li, Ma and Vitányi (2004).
As a second interpolation, in general, we also show that $\mathcal V_p$ is a metric, $1\le p\le \infty $, where $$ \begin{align*} & \varDelta_p(A,B)=(\lvert{B\setminus A}\rvert^p+\lvert{A\setminus B}\rvert^p)^{1/p}, \end{align*}$$$$ \begin{align*} & \mathcal V_p(A,B)=\frac{\varDelta_p(A,B)}{\lvert{A\cap B}\rvert + \varDelta_p(A,B)}. \end{align*}$$
A Retrospective Cohort Study of Burn Casualties Transported by the US Army Burn Flight Team and US Air Force Critical Care Air Transport Teams
Authors: Savell Shelia C , Howard Jeff T , VanFosson Christopher A , Medellín Kimberly L , Staudt Amanda M , Rizzo Julie A , Maddry Joseph K , Cancio Leopoldo C 
Published: September 2022

ABSTRACT

Introduction
The US Army Burn Center, the only burn center in the Department of Defense provides comprehensive burn care. The Burn Flight Team (BFT) provides specialized burn care during transcontinental evacuation. During Operations Iraqi and Enduring Freedom, burn injuries accounted for approximately 5% of all injuries in military personnel. To augment BFT capacity, US Air Force Critical Care Air Transport Teams (CCATTs) mobilized to transport burn patients. The purpose of this study was to describe critically ill, burn injured patients transported to the US Army Burn Center by BFT or CCATT, to compare and contrast characteristics, evacuation procedures, in-flight treatments, patient injuries/illnesses, and outcomes between the two groups.

Materials and Methods
We conducted a retrospective cohort study of CCATT and BFT patients, admitted to the burn ICU between January 1, 2001 and September 30, 2018. Patients with total body surface area burned (TBSA) &gt;30% were evacuated by BFT, while CCATT evacuated patients with ≤ 30% TBSA.

Results
Ninety-seven patients met inclusion criteria for this study. Of these, 40 (41%) were transported by the BFT and 57 (59%) were transported by CCATTs. Compared with patients transported by CCATTs, patients transferred by the BFT had higher median TBSA and full-thickness burn size, higher prevalence of chest, back and groin burns, and higher prevalence of inhalation injury. BFT patients had increased hospital days (62 vs. 37; P = .08), ICU days (29 vs. 12; P = .003) and ventilator days (14 vs. 6; P  &lt; .001). TBSA was the only variable significantly associated with ARDS (aOR = 1.04; 95% CI: 1.01, 1.08; P = 0.04), renal failure (aOR = 1.07; 95% CI: 1.03, 1.11; P = .002), and mortality (aOR = 1.08; 95% CI: 1.03, 1.13; P = .001).

Conclusions
Evacuation by the BFT was associated with increased ICU and ventilator days, increased mortality, and a greater risk for developing renal failure. The severity of injury/TBSA likely accounted for most of these differences.
Cosmic radiation backgrounds from primordial black holes
Authors: Ziparo F , Gallerani S , Ferrara A , Vito F 
Published: September 2022

ABSTRACT
Recent measurements of the cosmic X-ray background (CXB) and cosmic radio background (CRB) obtained with Chandra and ARCADE2 (Absolute Radiometer for Cosmology, Astrophysics, and Diffuse Emission) report signals in excess of those expected from known sources, suggesting the presence of a yet undiscovered population of emitters. We investigate the hypothesis that such excesses are due to primordial black holes (PBHs) that may constitute a substantial fraction of dark matter (DM). We present a novel semi-analytical model that predicts X-ray and radio emission due to gas accretion on to PBHs, assuming that they are distributed both inside DM haloes and in the intergalactic medium (IGM). Our model includes a self-consistent treatment of heating/ionization feedback on the surrounding environment. We find that (i) the emission from PBHs accreting in the IGM is subdominant at all times ($1{{\ \rm per\ cent}} \le I_{\rm IGM}/I_{\rm tot} \le 40{{\ \rm per\ cent}}$); (ii) most of the CXB/CRB emission comes from PBHs in DM mini-haloes (Mh ≤ 106 M⊙) at early epochs ($z$ &gt; 6). While a small fraction ($f_{\rm PBH} \simeq 0.3{{\ \rm per\ cent}}$) of DM in the form of PBHs can account for the total observed CXB excess, the CRB one cannot be explained by PBHs. Our results set the strongest existing constraint on fPBH ≤ 3 × 10−4 (30/MPBH) in the mass range of $1 \!-\! 1000 \, \mathrm{M}_\odot$. Finally, we comment on the implications of our results on the global H i 21 cm signal.
Transition from multipolar to dipolar dynamos in stratified systems
Authors: Zaire B , Jouve L , Gastine T , Donati J-F , Morin J , Landin N , Folsom C P 
Published: September 2022

ABSTRACT
Observations of surface magnetic fields of cool stars reveal a large diversity of configurations. Although there is now a consensus that these fields are generated through dynamo processes occurring within the convective zone, the physical mechanism driving such a variety of field topologies is still debated. This paper discusses the possible origins of dipole- and multipole-dominated morphologies using three-dimensional numerical simulations of stratified systems where the magnetic feedback on the fluid motion is significant. Our main result is that dipolar solutions are found at Rossby numbers up to 0.4 in strongly stratified simulations, where previous works suggested that only multipolar fields should exist. We argue that these simulations are reminiscent of the outlier stars observed at Rossby numbers larger than 0.1, whose large-scale magnetic field is dominated by their axisymmetric poloidal component. As suggested in previous Boussinesq calculations, the relative importance of inertial over Lorentz forces is again controlling the dipolar to multipolar transition. Alternatively, we find that the ratio of kinetic to magnetic energies can equally well capture the transition in the field morphology. We test the ability of this new proxy to predict the magnetic morphology of a few M dwarf stars whose internal structure matches that of our simulations and for which homogeneous magnetic field characterization is available. Finally, the magnitude of the differential rotation obtained in our simulations is compared to actual measurements reported in the literature for M dwarfs. In our simulations, we find a clear relationship between antisolar differential rotation and the emergence of dipolar fields.
On optimal temozolomide scheduling for slowly growing glioblastomas
Authors: Segura-Collar Berta , Jiménez-Sánchez Juan , Gargini Ricardo , Dragoj Miodrag , Sepúlveda-Sánchez Juan M , Pešić Milica , Ramírez María A , Ayala-Hernández Luis E , Sánchez-Gómez Pilar , Pérez-García Víctor M 
Published: September 2022

Abstract

Background
Temozolomide (TMZ) is an oral alkylating agent active against gliomas with a favorable toxicity profile. It is part of the standard of care in the management of glioblastoma (GBM), and is commonly used in low-grade gliomas (LGG). In-silico mathematical models can potentially be used to personalize treatments and to accelerate the discovery of optimal drug delivery schemes.

Methods
Agent-based mathematical models fed with either mouse or patient data were developed for the in-silico studies. The experimental test beds used to confirm the results were: mouse glioma models obtained by retroviral expression of EGFR-wt/EGFR-vIII in primary progenitors from p16/p19 ko mice and grown in-vitro and in-vivo in orthotopic allografts, and human GBM U251 cells immobilized in alginate microfibers. The patient data used to parametrize the model were obtained from the TCGA/TCIA databases and the TOG clinical study.

Results
Slow-growth “virtual” murine GBMs benefited from increasing TMZ dose separation in-silico. In line with the simulation results, improved survival, reduced toxicity, lower expression of resistance factors, and reduction of the tumor mesenchymal component were observed in experimental models subject to long-cycle treatment, particularly in slowly growing tumors. Tissue analysis after long-cycle TMZ treatments revealed epigenetically driven changes in tumor phenotype, which could explain the reduction in GBM growth speed. In-silico trials provided support for implementation methods in human patients.

Conclusions
In-silico simulations, in-vitro and in-vivo studies show that TMZ administration schedules with increased time between doses may reduce toxicity, delay the appearance of resistances and lead to survival benefits mediated by changes in the tumor phenotype in slowly-growing GBMs.
Nailfold capillaroscopy in diabetes mellitus: a case of neo-angiogenesis after achieving normoglycemia
Authors: Maldonado Genessis , Chacko Amala , Lichtenberg Robert , Ionescu Madalina , Rios Carlos 
Published: September 2022

ABSTRACT
Diabetes mellitus (DM) is a disease process characterized by a chronic hyperglycemic milieu that leads to micro and macrovascular complications, including diabetic retinopathy, diabetic nephropathy and diabetic neuropathy. During the last decade, researchers have used nail-fold capillaroscopy to study the microvascular alterations in rheumatologic diseases; however, the technology is gaining momentum in other disease processes that alter microvascular architecture. We observed a drastic improvement in the nail-fold capillary architecture in a patient with uncontrolled DM. After achieving excellent glycemic control 6 months after diagnosis, increased capillary density and evident rearrangement of the capillaries replaced the avascular areas and giant capillaries found at the time of diagnosis.
A rare presentation of scrub typhus: myocarditis, acute liver failure and leukemoid reaction
Authors: Mondal Md Karimulla , Ghosh Jayaditya , Bhattacharya Bhaswar , Chatterjee Nandini , Ghosh Soumitra 
Published: September 2022

ABSTRACT
Scrub typhus is a mite borne zoonosis, caused by Orientia tsutsugamushi, a gram-negative intracellular organism. This infection usually presents in high prevalence in the rural areas of East Asia and Western pacific islands. It usually presents with fever, chill, myalgia, headache, skin rashes, having pathognomonic and skin lesion i.e. eschar in ~10% cases in Indian subcontinent. It can present with life-threatening complications on occasional. The simultaneous presentation of more than two complications is uncommon, rarely reported in literature. Here we report a case of 37-year-old woman with acute febrile illness, complicated with myocarditis, acute liver failure and leukemoid reaction. She was diagnosed promptly and successfully treated with doxycycline with full recovery of the complications.
Moral Universalism and the Structure of Ideology
Authors: Enke Benjamin , Rodríguez-Padilla Ricardo , Zimmermann Florian 
Published: September 2022

Abstract
Throughout the Western world, people’s policy views are correlated across domains in a strikingly similar fashion. This article proposes that what partly explains the structure of ideology is moral universalism: the extent to which people exhibit the same level of altruism and trust towards strangers as towards in-group members. In new large-scale multinational surveys, heterogeneity in universalism descriptively explains why some people support redistribution, health care, environmental protection, affirmative action, and foreign aid, while others advocate for spending on the military, law enforcement, and border protection. Universalism is a substantially stronger predictor of policy views and ideological constraints than variables such as income, wealth, education, religiosity, or beliefs about government efficiency. Consistent with the idea that universalism shapes policy views, we further document that the left–right divide on redistribution, environmental protection, or foreign aid strongly attenuates or even reverses when people evaluate less universalist implementations of these policies.