bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence and risk factors of infusion reactions in patients with breast cancer administered pertuzumab combination chemotherapy
Authors: Tabuchi Yusuke , Tsujimoto Masayuki , Yamamoto Kosuke , Kosaka Tadashi , Sakaguchi Koichi , Dobuchi Naoya , Nishiguchi Kohshi , Shikata Keisuke 
Published: June 2022

Abstract
Purpose
Pertuzumab (Per) is a humanized monoclonal antibody used in combination with trastuzumab (Tra) in the treatment of human epidermal growth factor receptor-2 (HER2)-positive breast cancer. The administration of biologics, such as Tra and Per, frequently causes infusion reactions (IRs) with fever and chills. This study aimed to clarify the characteristics of and risk factors for IRs in Tra + Per combination therapy.
Methods
Between March 2013 and December 2019, 65 patients with breast cancer who started Tra + Per combination therapy were included in the study. The severity of IRs was graded according to the Common Terminology Criteria for Adverse Events (CTCAE) version 5.0.
Results
The incidence of IRs in the Tra + Per combination therapy was 47.7% (31/65). The severity of IRs in the Tra + Per combination therapy was Grade 1 (9 patients) and Grade 2 (22 patients). Lymphocyte counts were significantly different between the IR and non-IR groups in patients receiving Tra + Per combination therapy (univariate analysis, p = 0.005; multivariate analysis, p = 0.029).
Conclusion
Our study indicates that the incidence of IRs in Tra + Per combination therapy is relatively high, the severity of IRs in most patients is moderate or less, and the risk of IRs is higher in patients with higher lymphocyte counts.
A Case Report of Curative Drugs for Abdominal Aortic Dissection Secondary to Retroperitoneal Fibrosis
Authors: Zhang Chao , Liu Yong , Ma Ning 
Published: June 2022

Abstract
Symptoms caused by squeezing ureters and abdominal vessels usually occurs after the onset of retroperitoneal fibrosis. We report a rare case of localized aortic dissection in a patient with retroperitoneal fibrosis. A 64-year-old man presented with intermitted abdominal pain was diagnosed with localized abdominal aortic dissection secondary to retroperitoneal fibrosis. After the treatment of the glucocorticoid and cyclophosphamide, both localized abdominal aortic dissection and retroperitoneal fibrosis disappeared. Imaging techniques such as ultrasound have played an important role in the diagnosis and follow-up. This is a very rare case of localized abdominal aortic dissection secondary to retroperitoneal fibrosis and then successfully cured only by the glucocorticoid and cyclophosphamide.
Research on deformation mechanism of egg-shaped shell in granular medium bulging
Authors: Huang Jiadong , sun zhiying , Shi Zhengxian , Zhang Jian , Wang Yao , Zhang Quanda 
Published: June 2022

Abstract
The design of pressure hull structure plays an essential role in the research and development of underwater operation equipment such as deep-sea submersibles. As a bionic structure, egg-shaped shell has apparent advantages in the application of an underwater pressure hull. Still, the egg-shaped shell is a positive Gauss surface, so it is difficult to control the manufacturing accuracy and quality. In this paper, the technology of solid particle flexible medium forming and the principle of high pressure bulging in tubes are proposed to manufacture high-precision egg-shaped shell parts. By optimizing the shape of the punch structure, optimizing the process parameters, improving the stress and strain distribution of the egg-shaped shell, analyzing the deformation mechanism in the bulging process, and mastering the deformation and quality control methods of the granular medium flexible bulging of non-equal curvature tubular structures.
Human Papillomavirus-driven repression of NRF2 signaling confers chemosensitivity and predicts prognosis in head and neck squamous cell carcinoma
Authors: Ramesh Pushkal S. , Bovilla Venugopal R. , Swamy Vikas H. , Manoli Nandini N. , Dasegowda Krishnappa B. , Siddegowda Siddegowda M. , Chandrashekarappa Shilpa , Somasundara Vishveshwara M. , Devegowda Devanand , Thimmulappa Rajesh K. 
Published: June 2022

Abstract
Purpose
To investigate the role of NRF2 signaling in conferring superior prognosis in patients with HPV positive (HPV+ ve) head & neck squamous cell carcinomas (HNSCC) compared to HPV negative (HPV− ve) HNSCC and develop molecular markers for selection of HPV+ ve HNSCC patients for treatment de-escalation trials.
Methods
We compared NRF2 activity (NRF2, KEAP1, and NRF2-transcriptional targets), p16, and p53 levels between HPV+ ve HNSCC and HPV− ve HNSCC in prospective and retrospective tumor samples. Cancer cells were transfected with HPV E6/E7 plasmid to elucidate if HPV infection repress NRF2 activity and sensitize to an anticancer drug.
Results
Prospective analysis revealed a marked reduction in expression of NRF2, and its target antioxidant genes (NQO1, HMOX-1, GCLC, and GCLM) in HPV+ ve tumors compared to HPV− ve tumors. A retrospective analysis by IHC revealed significantly lower NQO1 in p16high tumors compared to p16low tumors and the NQO1 expression correlated negatively with p16 and positively with p53. Analysis of the TCGA database confirmed low constitutive NRF2 activity in HPV+ ve HNSCC compared to HPV− ve HNSCC and revealed that HPV+ ve HNSCC patients with ‘low NQO1’ expression showed better overall survival compared to HPV+ ve HNSCC patients with ‘high NQO1’ expression. Ectopic expression of HPV E6/E7 plasmid in cancer cells repressed constitutive NRF2 activity, increased KEAP1, reduced total GSH levels, and enhanced cytotoxic effects of cisplatin.
Conclusion
Low constitutive NRF2 activity contributes to better prognosis in patients with HPV+ ve HNSCC compared to HPV− ve HNSCC. Co-expression of p16high, NQO1low, and p53low could serve as a predictive biomarker for the selection of HPV+ ve HNSCC patients for de-escalation trials.
Improvement of body weight and nutritional status in gastric cancer patients enhances the efficacy of nivolumab therapy
Authors: Ikoma Tatsuki , Matsumoto Toshihiko , Kurioka Yusuke , Takatani Masahiro , Nagai Hiroki , Matsumoto Yusuke , Satake Hironaga , Yasui Hisateru 
Published: June 2022

Abstract
Background
Nivolumab improves the overall survival (OS) of patients with advanced gastric cancer (AGC) refractory to at least two previous chemotherapy regimens. Here, we investigated whether changes in body weight and nutrition from 1st -line chemotherapy to nivolumab could affect the efficacy of nivolumab.
Methods
We retrospectively examined patients with AGC treated with nivolumab between July 2016 and August 2020. This study examined the correlation between changes in body weight and nutritional status up to the start of nivolumab treatment, and the overall survival (OS) and progression-free survival (PFS) after the initiation of nivolumab treatment. Nutritional status was examined using the C-reactive protein/albumin ratio (CAR).
Results
Ninety-eight patients were enrolled. In our study, a loss in body weight (LBW) from the start of the first treatment of < 4.5% led to OS prolongation. The median OS in the LBW < 4.5% group and in the LBW ≥ 4.5% group was 11.4 months (95% confidence interval [CI] 6.6–14.3) and 3.6 (95%CI 2.2–5.1) months, respectively (hazard ratio [HR] 0.42; 95%CI 0.26–0.66; p < 0.001). Similarly, the change in CAR from first-line chemotherapy (ΔCAR) affected OS, and the ΔCAR < 0.01 group had a better prognosis than ΔCAR ≥ 0.01 group. The median OS in the ΔCAR < 0.01 group and in the ΔCAR ≥ 0.01 group was 9.4 (95%CI, 5.1–13.7) and 4.5 (95%CI 4.0–5.0) months, respectively (HR 0.59; 95%CI 0.37–0.93; p = 0.002). Moreover, the median OS in the group with both LBW < 4.5% and ΔCAR < 0.01 was 12.9 months (95%CI 6.8–19.0). Furthermore, the LBW < 4.5% group showed statistically significantly better PFS (median PFS 2.7 vs. 2.0 months; HR 0.63, 95%CI 0.42–0.94; p = 0.021).
Conclusion
Our study suggested that LBW and deterioration of nutritional status from the start of first-line chemotherapy are poor prognostic factors in AGC patients who received nivolumab as third- or later-line therapy. Early intervention to maintain body weight and nutritional status from the initiation of prior chemotherapy may improve the efficacy of immune checkpoint inhibitors.
Mathematical Analysis in the design of Digital Artery-Based V-Y Advancement flap in Treating Proximal Interphalangeal Joint Flexion Contracture
Authors: Su Po Han , Hsu Cheng-En , Ho Tsung-Yu , Wei Bor-han , Wang Wei-Chih , Chiu Yung-Cheng 
Published: June 2022

Abstract
Background: The digital artery-based V-Y advancement flap is a widely used flap for soft tissue coverage in the treatment of flexion contracture of the proximal interphalangeal (PIP) joint. A standard method for the flap design and a mathematical method to predict the advance distance have not been well established. In this study, we proposed a simplified method for the design of V-Y advancement flaps based on digital arteries and used a geometric model to predict the advance distance for the flexion contracture correction surgery.Methods: According to the general concept of hand flap design and law of cosine, we proposed three principles in the design of the digital artery-based V-Y advancement flap that should be followed. Since 2021 to 2022, finger geometric data of 120 fingers (index, middle, ring, and small fingers) from 30 healthy participants were collected and analysed to evaluate the necessary advance distance and flap tip angle for PIP flexion contracture correction of different fingers by our flap design method.Results: The middle finger needed a significantly longer advance distance compared to other fingers in the same degree flexion contracture correction. The ring finger had the largest length-to width ratio and smallest flap tip angle among the four fingers in the V-Y flap design. No vertical scar crossed the flexion creases and flap tip angle <20° was found in the tentative V-Y flap design for the 120 fingers.Conclusions: Our flap design method provides a proper advance distance and flap length-to-width ratio without common skin complications in the flap design for PIP flexion contracture of index, middle, ring and small fingers. This geometric model provides a mathematical basis for prediction of advance distance and flap tip angle in the design of a digital artery-based V-Y advancement flap.
Preparation and optimization of photocatalytic polyacrylic acid/guar gum/TiO 2 hydrogels for absorption and removing of methylene blue under Visible and UV irradiation
Authors: Naserzade Seyyed Masud , Shahrousvand Mohsen , Mohammadi-Rovshandeh Jamshid , Basati Hojjat 
Published: June 2022

Abstract
Optimized polyacrylic acid/guar gum/Titanium dioxide (PAA/GG/TiO2) hydrogels were fabricated according to Design-Expert software. The intermolecular structures of the hydrogels and the presence of nanoparticles were examined by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). To achieve dye absorption rate, kinetic models such as pseudo-first-order, pseudo-second-order, Elovich, and intraparticle diffusion (Weber-Morris), had been investigated in visible light and UV radiation at an initial concentration of MB 20 (ppm) for one day. Diagrams of absorption isotherms in visible light and UV irradiation at 20-60 (ppm) concentrations were evaluated based on the models of Freundlich, Langmuir (Hanes-Woolf), Temkin, and Dubinin-Radushkevic. The hydrogel containing crosslinker (4 mol %), TiO2 (5 wt %), guar gum (2 wt %), and into the alkaline medium (pH=8) was selected as superlative optimal status. The sample was examined for mapping of x-ray energy diffraction (EDS) according to different elements densities of various circumstances to show the degradation or absorption of methylene blue. The results showed that the prepared hydrogels have a good efficiency for removing cationic contaminants from the aqueous medium and this property is intensified by UV radiation.
Sign Language Gesture Recognition from Spatio-temporal Features
Authors: Rajput Rajesh Singh , Purushothaman Anurenjan , S. Jeena R. , Manu Chippy M , G Sreeni K. 
Published: June 2022

Abstract
Sign Language is the primary mode of communication for the hearing impaired community and between the community and the outside world. This paper proposes a vision-based sign language gesture recognition model to identify the sign gesture (word) from a hand gesture video. The proposed model consists of three modules: Pre-processing, Convolutional Neural Network, Recurrent Neural Network. Pre-processing module is used to extract the frames, segment the region of interest, and convert them into a grayscale image. Convolutional Neural Network is used to extract the spatial features for each frame and each video is represented by a sequence of spatial features. Recurrent Neural Network recognizes the gestures based on spatio-temporal relation in the sequence of features. The evaluation of the proposed model is done on two different datasets. With Argentinian Sign Language (LSA 64), the model is able to achieve an accuracy of 100%. The model achieved an accuracy of 97.70% with the data set: Indian Sign Language (ISL) for Emergency Situations.
NanoSTR: A Method for Detection of Target Short Tandem Repeats Based on Nanopore Sequencing Data
Authors: Lang Jidong , Xu Zhihua , Wang Yue , Sun Jiguo , Yang Zhi 
Published: June 2022

Abstract
Background: Short tandem repeats (STRs) are widely present in the human genome. Studies have confirmed that STRs are associated with more than 30 diseases, and they have also been used in forensic identification and paternity testing. However, there are few methods for STR detection based on nanopore sequencing due to the challenges posed by the sequencing principles and the data characteristics of nanopore sequencing.Results: We developed NanoSTR for detection of target STR loci based on the length-number-rank (LNR) information of reads. NanoSTR can be used for STR detection and genotyping based on long-read data from nanopore sequencing with improved accuracy and efficiency compared with other existing methods, such as Tandem-Genotypes and TRiCoLOR. NanoSTR showed 100% concordance with the expected genotypes using error-free simulated data. Besides, NanoSTR also showed 86.36% and 73.48% concordance using the standard samples 9948 and 2800M with MinION sequencing platform, respectively. Meanwhile, the concordance with Qnome-3841 sequencing platform were 71.97% and 53.03%, respectively.Conclusions: NanoSTR showed high performance for detection of target STR markers. Although NanoSTR needs further optimization and development, it is useful as an analytical method for the detection of STR loci by nanopore sequencing. This method adds to the toolbox for nanopore-based STR analysis and expands the applications of nanopore sequencing in scientific research and clinical scenarios.
Mutant p53-driven lncRNA LINC00857 promotes pancreatic cancer metastasis through OTUB1- mediated FOXM1 deubiquitination
Authors: Zhang Weifan , Qian Weikun , Gu Jingtao , Gong Mengyuan , Zhang Wunai , Zhang Simei , Zhou Cancan , Jiang Jie , Han Liang , Wu Zheng , Ma Qingyong , Wang Zheng 
Published: June 2022

Abstract
Background
p53 mutations are considered to be the second most common type of mutation in PC, and significantly promote its metastasis. However, the underlying molecular mechanisms, especially the regulatory relationship with long noncoding RNAs (lncRNAs) remain unclear.
Methods
Based on TCGA and GTEx databases and integrating bioinformatics analyses, the overexpression of lncRNA LINC00857 in PC tissues was detected and further validated in a cohort of PC tissues. The effects of LINC00857 on EMT, migration and invasion were determined by in vitro and in vivo experiments. RNA pull-down, RNA immunoprecipitation (RIP), chromatin isolation by RNA purification (ChIRP) and fluorescence in situ hybridization (FISH) were carried out to reveal the interaction between LINC00857 and FOXM1, OTUB1. Chromatin immunoprecipitation (ChIP) and Luciferase reporter assay were used to evaluate the regulatory effect of mutant p53 on LINC00857.
Results
lncRNA LINC00857 exhibited a significantly elevated level in PC and was involved in poor prognosis, and its expression was significantly upregulated in the p53 mutant group compared with the p53 wild-type group. Moreover, LINC00857 remarkably suppressed tumour metastasis in vitro and in vivo in a FOXM1 dependent manner. Mechanistically, LINC00857 simultaneously bound to FOXM1 and the deubiquitinase OTUB1, serving as a protein scaffold to enhance the interaction between FOXM1 and OTUB1, which inhibited FOXM1 degradation through the ubiquitin–proteasome pathway. Interestingly, we found that mutant p53 promoted the transcription of LINC00857 by binding to its promoter region. Finally, atorvastatin, a commonly applied lipid-lowering drug in the clinic, was suggested to inhibit PC metastasis by inhibiting the mutant p53-LINC00857 axis.
Conclusions
Taken together, our results uncovered new insights into the biology driving PC metastasis and indicated that the mutant p53-LINC00857 axis might serve as a novel therapeutic target for PC metastasis.
Clinical and statistical analysis of predictors oflethal outcomes in tuberculosis patients treated in medical units of the Russian Federal penitentiary system
Authors: Vostroknutov M. E. , Sterlikov S. A. , Kudrina V. G. , Popova N. M. 
Published: June 2022

The objective: to determine risk factors of lethal outcomes (predictors) in tuberculosis patients treated with chemotherapy regimens I, II, III during conﬁnement in the Russian penitentiary system.Subjects and Methods: data on 6,069 patients registered for treatment in 2019 and 2020 were studied. Univariate and multivariate analyses were performed.Results. The following factors were found to have eﬀect on mortality: age (aOR = 1.05; 95% CI 1.03-1.06), multiple localizations of tuberculosis (aOR = 11.08; 95% CI 5.12-24.20), concurrent HIV infection (aOR = 9.13; 95% CI 6.90-12.24), and positive sputum microscopy at registration (aOR = 5.18; 95% CI 3.93-6.81). The patient gender was found to have no eﬀect (OR = 0.9; 95% CI 0.4-1.9; p = 1).
Comparative analysis of eﬃcacy and safety of various anti-tuberculosis therapy regimens in patients with MDR/XDR tuberculosis
Authors: Myshkova E. P. , Petrenko T. I. , Kolpakova T. A. 
Published: June 2022

The objective: to evaluate the eﬃcacy and safety of various anti-tuberculosis therapy regimens for multiple drug resistant (MDR) and extensive drug resistant pulmonary tuberculosis in the intensive phase of treatment lasting for 8 months minimum.Subjects and Methods. A single-center cohort prospective-retrospective clinical study was conducted, which included 166 patients aged 18 to 70 years with active pulmonary tuberculosis and proven MDR of Mycobacterium tuberculosis, who received chemotherapy according to regimens IV and V. The treatment regimen of patients in Group I (n = 96) included modern anti-tuberculosis drugs (TBdrugs) – bedaquiline, linezolid, respiratory ﬂuoroquinolone (levoﬂoxacin, moxiﬂoxacin, sparﬂoxacin), cycloserine, pyrazinamide, and perchlozone at a dose of 8-10 mg/kg, 1 time per day after meals during the intensive phase of treatment, that was at least 8 months. Patients from Group II (n = 70) received a respiratory ﬂuoroquinolone (levoﬂoxacin, moxiﬂoxacin, sparﬂoxacin), aminoglycoside (amikacin), cycloserine, pyrazinamide, ethambutol, and prothionamide.Results. After 3 months of treatment, symptoms of intoxication disappeared in 22 (81.5%) patients in Group I, and only 41 (61.2%) in Group II (p = 0.04; TTF). In patients of Group I versus Group II, the body temperature returned to normal within a shorter time: 2.8 and 4.3 weeks, respectively (p < 0.05). By the end of the intensive phase of treatment (8 months), sputum conversion conﬁrmed by culture was observed more often in patients of Group I compared to Group II (85 and 80%, pχ2 = 0.003). Adverse events (AE) caused by TB drugs in Group I were observed signiﬁcantly more often versus Group II: gastroenterological adverse events (pχ2 = 0.05), cardiac adverse events (pTTP = 0.05), and endocrinological adverse events (pTTP = 0.05). Neurotoxic AEs tended to develop more often in Group I (pTTP = 0.06). Ototoxic AEs were more frequently observed in Group II where the treatment regimens included aminoglycosides (pχ2 = 0.05). To maintain adequate chemotherapy regimens IV or V over long-term treatment, MDR tuberculosis patients need continuous monitoring of AEs, intravenous administration of two or three TB drugs, and timely therapy to manage manifestations of AEs.
Analysis of frequency ofgenotypes and alleles of rs2227983 polymorphism of <i>EGFR</i> gene among patients with allergic bronchial asthma with various levels of control
Authors: Cherkashina I. I. , Nikulina S. Yu. , Averyanov A. B. , Kuznetsova E. Yu. , Nikolaeva L. V. 
Published: June 2022

The objective: exploring the frequency of circulation of genotypes and alleles of rs2227983 polymorphism of EGFR gene among patients with allergic bronchial asthma with various levels of control.Subjects and Methods. The study includes adult individuals (n = 396) divided into two groups: Group 1 (main) – individuals with allergic bronchial asthma (ABA), Group 2 is a control one. ABA Group consisted of 179individuals (118 women и 61 men) aged 37.4 ± 14.2 years. Control Group consisted of 217 individuals (110 women и 107 men) aged 30.0±9.1years. All subject included in ABA Group and Control Group underwent molecular genetic tests for rs2227983 polymorphism of EGFR gene using polymerase chain reaction.Results. The comparison of distribution of gene EGFR genotypes showed statistically signiﬁcant predominance of genotype AGcarriers in the group of patients with ABA (48.6%), versus Control Group (36.9%); p < 0.05. Besides that, there is a predominance of number of carriers of heterozygote genotype АG of EGFR gene among patients with ABA (57.7%) with a controlled course of the disease versus Control Group (36.9%); p < 0.05. Also, it has been found out that rs2227983 genotype of EGFR gene was more frequent in women with controlled ABA (67.4%) compared to women from Control Group (32.7%); p < 0.05.Conclusion. The results of the conducted study allowed analyzing the connection of single nucleotide rs2227983 polymorphism ofEGFR gene with ABA including various levels of control of the course of the disease. Genotype АG of single nucleotide polymorphism rs2227983 of EGFR gene increases the risk of developing ABA. Alongside this, АG genotype of EGFR gene is correlated with the controlled course of ABA.