bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stabilization of CCDC102B by Loss of RACK1 Through the CMA Pathway Promotes Breast Cancer Metastasis via Activation of the NF-κB Pathway
Authors: Si Jing , Guo Rong , Xiu Bingqiu , Chi Weiru , Zhang Qi , Hou Jianjing , Su Yonghui , Chen Jiajian , Xue Jingyan , Shao Zhi-Ming , Wu Jiong , Chi Yayun 
Published: July 2022

BackgroundBreast cancer is one of the leading causes of cancer-related death among women, and the pathological status of axillary lymph nodes is an important predictor of prognosis. However, the mechanism involved in this early stage of metastasis remains largely unknown.MethodsMicroarray analysis was used to carry out differential genomics analyses between matched pairs of metastatic sentinel lymph node tissues and breast primary tumors. The CRISPR/Cas9 gene editing system was used for in vivo screening by transplanting a loss-of-function cell pool into immunocompromised mice. MAGeCK was used to analyze the screening results. Survival analysis was performed via the Kaplan–Meier method. Cell proliferation, wound healing, migration and invasion assays were performed to confirm the phenotype. A tail vein model and subcutaneous xenotransplanted tumor model were used for the in vivo study. The relationship between coiled-coil domain containing 102B (CCDC102B) and receptor for activated C kinase 1 (RACK1) was examined using coimmunoprecipitation, mass spectrometry, nuclear protein extraction and immunofluorescence assays. The primary biological functions and pathways related to CCDC102B were enriched by RNA sequencing.ResultsWe identified CCDC102B through screening and found that it was significantly upregulated in metastatic lesions in lymph nodes compared to matched primary tumors. Increased expression of CCDC102B promoted breast cancer metastasis in vitro and in vivo. Additionally, high expression of CCDC102B was correlated with poor clinical outcomes in breast cancer patients. We further identified that CCDC102B was stabilized by the loss of RACK1, a protein negatively correlated with breast cancer metastasis. Mechanistically, we found that RACK1 promoted CCDC102B lysosomal degradation by mediating chaperone-mediated autophagy (CMA). The aggressive behavior of CCDC102B in breast cancer cells could be reversed by the expression of RACK1. Moreover, CCDC102B was correlated with the significant enrichment of NF-κB pathway components. Overexpressing CCDC102B led to less interaction between RACK1 and IKKa. Thus, CCDC102B positively regulates the NF−κB pathway by interacting with RACK1.ConclusionTaken together, our findings uncover a novel role of CCDC102B in breast cancer metastasis. CCDC102B serves as a potential metastasis promoter by regulating the activation of the NF-κB pathway and can be degraded by RACK1 via CMA.
Construction of immunotherapy-related prognostic gene signature and small molecule drug prediction for cutaneous melanoma
Authors: Xing Jiahua , Jia Ziqi , Li Yan , Han Yan 
Published: July 2022

BackgroundCutaneous melanoma (CM), a kind of skin cancer with a high rate of advanced mortality, exhibits a wide variety of driver and transmitter gene alterations in the immunological tumor microenvironment (TME) associated with tumor cell survival and proliferation.MethodsWe analyzed the immunological infiltration of TME cells in normal and malignant tissues using 469 CM and 556 normal skin samples. We used a single sample gene set enrichment assay (ssGSEA) to quantify the relative abundance of 28 cells, then used the LASSO COX regression model to develop a riskScore prognostic model, followed by a small molecule drug screening and molecular docking validation, which was then validated using qRT-PCR and IHC.ResultsWe developed a prognosis model around seven essential protective genes for the first time, dramatically elevated in tumor tissues, as did immune cell infiltration. Multivariate Cox regression results indicated that riskScore is an independent and robust prognostic indicator, and its predictive value in immunotherapy was verified. Additionally, we identified Gabapentin as a possible small molecule therapeutic for CM.ConclusionsA riskScore model was developed in this work to analyze patient prognosis, TME cell infiltration features, and treatment responsiveness. The development of this model not only aids in predicting patient response to immunotherapy but also has significant implications for the development of novel immunotherapeutic agents and the promotion of tailored treatment regimens.
Expression and Possible Role of Silent Mating Type Information Regulation 2 Homolog 1 in Post-necrotizing Enterocolitis Stricture in vivo and in vitro
Authors: Chen Rui , Lv Chengjie , Zhao Yun , Gu Weizhong , Zhang Luyin , Shi Bo , Tou Jingfa 
Published: July 2022

PurposeTo investigate the expression and possible role of Sirtuin1 or Silent mating–type information regulation 2 homolog-1 (SIRT1) in post-necrotizing enterocolitis stricture.Materials and MethodsThe expression characteristics of SIRT1 and TGF-β1 in post-necrotizing enterocolitis stricture were detected by immunohistochemistry. The siRNA-SIRT1 was used to inhibit the expression of SIRT1 in intestinal epithelial cells-6 (IEC-6), and qRT-PCR, WB, and ELISA were utilized to detect the changes of Transforming growth factor-β1 (TGF-β1), nuclear factor (NF)-κB, tumor necrosis factor-α (TNF-α), tight junction protein-1 (ZO-1), and vascular endothelial growth factor (VEGF) expressions. The IEC-6 cell proliferation and migration ability were tested via CCK8 kit and Transwell test. The expression of E-cadherin and Vimentin in cells was detected by immunofluorescence.ResultsThe CRP, IL-6, IL-10, and IFN-γ in the serum of Necrotizing enterocolitis (NEC) intestinal stenosis patients were significantly higher than the reference values. The SIRT1 protein was under-expressed and the TGF-β1 protein was overexpressed in NEC intestinal stenosis tissue. And the expression of SIRT1 was negatively correlated with TGF-β1. At the time of diagnosis of NEC, the expression of SIRT1 decreased in children with respiratory distress syndrome and CRP level increased. After inhibiting the expression of SIRT1 in IEC6 cells, the expression levels of TGF-β1, Smad3, and NF-κB were decreased, and the expression of ZO-1 was also decreased. The proliferation and migration ability of IEC6 cells was decreased significantly, and the expression of E-cadherin and Vimentin proteins in IEC6 cells did not change significantly.ConclusionPromotion of intestinal fibrosis by inflammation may be the mechanism of post-necrotizing enterocolitis stricture. SIRT1 may be a protective protein of NEC. The probable mechanism is that SIRT1 can regulate intestinal fibrosis and can protect the intestinal mucosal barrier function to participate in the process of post-necrotizing enterocolitis stricture.
Buddhist-like opposite diminishing and non-judging during ketamine infusion are associated with antidepressant response: an open-label personalized-dosing study
Authors: Stocker Kurt , Hartmann Matthias , Reissmann Steffen , Kist Andreas , Liechti Matthias E. 
Published: July 2022

Background: Cognition that is not dominated by thinking in terms of opposites (opposite diminishing) or by making judgments (non-judging) can be found both in Buddhist/mindfulness contexts and in mental states that are fostered by dissociative psychedelics (N-methyl-D-aspartate antagonists) such as ketamine. Especially for the Buddhist/mindfulness case, both opposite diminishing and non-judging have been proposed to relate to mental well-being. Whether ketamine-occasioned opposite diminishing and/or non-judging relate to increased mental well-being in the form of antidepressant response is unknown, and was investigated in the present study.Methods: In this open-label outpatient study, the dose level and frequency for the ketamine infusions were adjusted individually in close consultation with the patients suffering from depression with the overall goal to maximize antidepressant benefits—a novel dose regimen that we term personalized antidepressant dosing. In general, treatment started with an initial series of ketamine infusions with a dosage of 0.5 mg/kg body weight and was then adjusted (usually increased). A possible relationship between ketamine-induced antidepressant benefits and retrospectively reported peri-infusion experiences of opposite diminishing and non-judging was assessed based on a total of 45 ketamine-infusion treatment sessions from 11 different patients suffering from depression. Opposite diminishing and non-judging were measured with the two items from the Altered States of Consciousness Inventory (ASCI) that measure these concepts. Depression was measured with the Beck Depression Inventory (BDI-II).Results: Peri-infusion experiences of both opposite diminishing and non-judging were associated with antidepressant responses confirming our hypothesis. Furthermore, opposite diminishing and non-judging were closely related to one another while relating to antidepressant response in distinguishable ways.Conclusion: Future controlled randomized trials with dissociative and other psychedelics and with a larger number of participants are needed to establish the possible link of psychedelically induced opposite diminishing and non-judging with an antidepressant response more firmly.
Activation of liver X receptors protects oligodendrocytes in CA3 of stress-induced mice
Authors: Zhu Peilin , Tang Jing , Liang Xin , Luo Yanmin , Wang Jin , Li Yue , Xiao Kai , Li Jing , Deng Yuhui , Jiang Lin , Xiao Qian , Qi Yingqiang , Xie Yuhan , Yang Hao , Zhu Lin , Tang Yong , Huang Chunxia 
Published: July 2022

Depression is a complex disorder that is associated with various structural abnormalities. Oligodendrocyte (OL) dysfunction is associated with the pathogenesis of depression and the promotion of hippocampal oligodendrocyte maturation and myelination could be a novel therapeutic strategy for ameliorating depressive behaviors. Recent studies have shown that activation of liver X receptors (LXRs) by GW3965 improves depressive phenotypes, but the effects of GW3965 on OL function and myelination in the hippocampus of depression remain relatively unclear. To address this issue, we investigated the effects of GW3965 on mature OL in the hippocampus and on the myelin sheaths of mice subjected to chronic unpredictable stress (CUS). Behavioral tests were performed to assess depressive behaviors. Then, the number of mature OLs (CC1+) in each hippocampal subregion was precisely quantified with immunohistochemical and stereological methods, and the density of newborn mature OLs (BrdU+/Olig2+/CC1+ cells) in each hippocampal subregion was quantified with immunofluorescence. In addition, myelin basic protein (MBP) staining intensity in the cornu ammonis 3 (CA3) region was assessed by using immunofluorescence. We found that both the number of CC1+ OLs and the density of BrdU+/Olig2+/CC1+ cells were obviously decreased in each hippocampal subregion of mice subjected to CUS, and 4 weeks of GW3965 treatment reversed these effects only in the CA3 region. Furthermore, the decreased MBP expression in the CA3 region of mice subjected to CUS was ameliorated by GW3965 treatment. Collectively, these results suggested that improvement of OL maturation and enhancement of myelination may be structural mechanisms underlying the antidepressant effects of LXR agonists.
Double-Pass Multiple-Plate Continuum for High-Temporal-Contrast Nonlinear Pulse Compression
Authors: Chen Bo-Han , Su Jia-Xuan , Guo Jhan-Yu , Chen Kai , Chu Shi-Wei , Lu Hsuan-Hao , Lu Chih-Hsuan , Yang Shang-Da 
Published: July 2022

We propose a new architecture, double-pass multiple-plate continuum (DPMPC), for nonlinear pulse compression. In addition to having a smaller footprint, a double-pass configuration is designed to achieve substantial bandwidth broadening without incurring noticeable higher-order dispersion, thus improving the temporal contrast over those of the traditional single-pass geometry when only the quadratic spectral phase can be compensated. In our proof-of-concept experiment, 187 μJ, 190-fs Yb-based laser pulse is compressed to 20 fs with high throughput (75%), high Strehl ratio (0.76), and excellent beam homogeneity by using DPMPC. The subsequently generated octave-spanning spectrum exhibits a significantly raised blue tail compared with that driven by pulses from a single-pass counterpart.
The impacts of mind-wandering on flow: Examining the critical role of physical activity and mindfulness
Authors: Deng Yu-Qin , Zhang Binn , Zheng Xinyan , Liu Ying , Wang Xiaochun , Zhou Chenglin 
Published: July 2022

BackgroundIndividuals with mind-wandering experience their attention decoupling from their main task at hand while others with flow experience fully engage in their task with the optimum experience. There seems to be a negative relationship between mind-wandering and flow. However, it remains unclear to what extent mind-wandering exerts an impact on flow. And it is also elusive whether physical activity and mindfulness, which are as important factors that affected individuals’ attentional control and psychological health, are beneficial in explaining the association between mind-wandering and flow. The current study investigated the relationship between mind-wandering and flow, and the potential mediation effects of physical activity and mindfulness in this association.MethodsA cross-sectional exploratory study design, including multiple scales such as the Mind-Wandering Questionnaire (MWQ), the International Physical Activity Questionnaire Short Form (IPAQ), Mindfulness Attention and Awareness Scale (MAAS), and the Short Dispositional Flow Scale (S-DFS) was applied. Descriptive statistics and bivariate correlation coefficients were applied in the analysis of these data. A multiple mediation model was used to examine the relationships between mind-wandering, flow, physical activity, and mindfulness.ResultsMind-wandering was inversely associated with physical activity, mindfulness and flow, respectively; and flow was positively related to physical activity and mindfulness, respectively. Moreover, multiple mediation results demonstrated that physical activity and mindfulness, respectively, mediated the relationship between mind-wandering and flow.ConclusionThese findings are helpful to understand how our minds attend to the present moment, and the crucial roles of physical activity and mindfulness in the association between mind-wandering and flow. An implication of these is the possibility that the effective strategies aimed at enhancing both the levels of physical activity and mindfulness are needed to reduce the negative impact of mind-wandering on flow.
The interplay of Criterion A of the Alternative Model for Personality Disorders, mentalization and resilience during the COVID-19 pandemic
Authors: Maerz Jeff , Buchheim Anna , Rabl Luna , Riedl David , Viviani Roberto , Labek Karin 
Published: July 2022

Background and aimsThe COVID-19 pandemic has been accompanied by a worsening of mental health levels in some, while others manage to adapt or recover relatively quickly. Transdiagnostic factors such as personality functioning are thought to be involved in determining mental health outcomes. The present study focused on two constructs of personality functioning, Criterion A of the Alternative Model for Personality Disorders (AMPD, DSM-5) and mentalization, as predictors of depressive symptoms and life satisfaction during the COVID-19 pandemic. A second focus of the study was to examine whether this relationship was mediated by resilience.MethodsLinear regression analyses were used to examine the relationship between personality functioning measured by Criterion A (AMPD, DSM-5) and mentalizing abilities as predictors, and depression and life satisfaction as mental health outcomes. To assess the hypothesis that this relationship is mediated by resilience a structural equation modeling approach was conducted. Data from N = 316 individuals from the general population were collected.ResultsLinear regression models revealed highly significant associations between Criterion A/mentalization and both outcome measures. Structural equation models showed a significant partial mediation by resilience of these relationships.ConclusionOur results support the hypothesis that mentalizing serves as a protective function by promoting resilience to the impact of stress and threats. Criterion A and mentalization performed similarly as predictors of mental health outcomes, providing empirically overlapping operationalizations of personality functioning. This finding emphasizes the importance of personality functioning in positive and negative mental health outcomes. Furthermore, our results are consistent with a mediating role of resilience.
Intermittent Theta Burst Stimulation vs. High-Frequency Repetitive Transcranial Magnetic Stimulation in the Treatment of Methamphetamine Patients
Authors: Liu Qingming , Sun Huimeng , Hu Yitian , Wang Qiongyao , Zhao Zhiyong , Dong Da , Shen Ying 
Published: July 2022

Background and AimsIn this brief report, we compare the effectiveness and safety of intermittent theta burst stimulation (iTBS) and conventional 10 Hz repetitive transcranial magnetic stimulation (rTMS) in patients with methamphetamine use disorder (MAUD). Our study suggests that iTBS would also reduce drug craving in patients with MAUD just as the 10 Hz; thus, there may be no difference in treatment effects between these two methods.MethodsIn total twenty male methamphetamine (MA) addicts were randomly assigned to iTBS (n = 10) or 10 Hz (n = 10) groups for 12 treatments. Cue-evoked cravings, anxiety, depression, and withdrawal symptoms were measured at baseline before the first treatment, and post-tests after days 10, 15, and 20.ResultsThe results showed that iTBS and 10 Hz treatment had similar effectiveness in reducing cue-induced craving in male addicts for MA. Both 10 Hz and iTBS improved withdrawal symptoms of patients with MAUD.ConclusionsIntermittent theta burst stimulation may be similar in effectiveness as 10 Hz in treating patients with MAUD. The clinical usefulness of rTMS could be improved substantially because of the increase in its capacity, cost, and accessibility. Importantly, the effectiveness of rTMS in the treatment of patients with MAUD is not yet proven, and should be tested in the large double-blind sham-controlled studies.
Addiction in the time of COVID-19: Longitudinal course of substance use, psychological distress, and loneliness among a transnational Tyrolean sample with substance use disorders
Authors: Lommer Kilian , Schurr Timo , Frajo-Apor Beatrice , Plattner Barbara , Chernova Anna , Conca Andreas , Fronthaler Martin , Haring Christian , Holzner Bernhard , Macina Christian , Marksteiner Josef , Miller Carl , Pardeller Silvia , Perwanger Verena , Pycha Roger , Schmidt Martin , Sperner-Unterweger Barbara , Tutzer Franziska , Hofer Alex 
Published: July 2022

IntroductionNext to an increased use of alcohol, the current pandemic has been associated with increased psychological distress among the general population. Research on its effects on individuals suffering from substance use disorders (SUD) is scarce. This study aimed at expanding the existing literature on this topic with a focus on the impact of loneliness and perceived social support.MethodsSixty-eight people diagnosed with SUD according to ICD-10 from the Austrian state of Tyrol and from the Italian Province of South Tyrol who had been treated in a psychiatric hospital in 2019 and one hundred and thirty-six matched reference subjects of the same regional background participated in an online survey. Sociodemographic variables and scores on the Brief Symptom Checklist, the Three-Item Loneliness Scale, and the Multidimensional Scale of Perceived Social Support were collected at baseline and 5 months thereafter. Baseline took place after the first wave, while follow-up largely coincided with the second wave of the pandemic.ResultsAmong both patients and the matched reference group, substance use as a means to feel better facing the pandemic rose and predicted higher levels of psychological distress. Patients were less likely to receive specific care at follow-up than at baseline and presented with a significantly higher prevalence of clinically relevant psychological distress and loneliness than the matched reference group at both assessment times. Among both groups, psychological burden remained unchanged over time. Perceived social support was generally significantly higher in the matched reference group than in patients. Loneliness and, to a lesser degree, low perceived social support predicted psychological distress.ConclusionThese findings emphasize the need of preventive and educational measures regarding substance use behavior for both individuals suffering from SUD and those without mental health disorders.
Prevalence and factors associated with comorbid depressive symptoms among people with low back pain in China: A cross-sectional study
Authors: He Chunxia , Chen Hongxiu , Guo Ling , Xu Lisheng , Liu Qingquan , Zhang Jiali , Hu Xiuying 
Published: July 2022

BackgroundLow back pain is a common medical condition among the general population that is associated with many adverse health effects when comorbid with depressive symptoms. However, little is known about depressive symptoms in the population with low back pain in China. Our study evaluated the prevalence of depressive symptoms and analyzed the factors associated with this condition in the Chinese population with low back pain.MethodsWe conducted a cross-sectional analysis of data from the 2018 China Health and Retirement Longitudinal Study. We collected low back pain information for each participant and identified depressive symptoms using the brief version of the Center for Epidemiologic Studies Depression Scale. A wide range of sociodemographic and health-related characteristics of the subjects were extracted. We measured the prevalence of depressive symptoms comorbid with low back pain and analyzed the associated factors by multiple logistic regression.ResultsA total of 5,779 respondents aged 45 and over with low back pain formed the sample, 41.8% of whom reported depressive symptoms. Multiple logistic regression analysis indicated greater vulnerability to depressive symptoms among females (OR = 1.41, 95% CI, 1.16–1.73), relatively younger persons (60–74 years: OR = 0.72, 95% CI, 0.63–0.83; ≥ 75 years: OR = 0.62, 95% CI, 0.49–0.79, reference: 45–59 years), those from the central and western regions (central: OR = 1.39, 95% CI, 1.18–1.64; western: OR = 1.56, 95% CI, 1.33–1.83), participants with extremely short sleep duration (OR = 2.74, 95% CI, 2.33–3.23), those with poor self-perceived health status (OR = 2.91, 95% CI, 2.34–3.63,), multisite pain (OR = 1.54, 95% CI, 1.20–1.98) and disability in activities of daily living (Basic: OR = 1.70, 95% CI, 1.47–1.98; Instrumental: OR = 1.95, 95% CI, 1.70–2.24).ConclusionDepressive symptoms were highly prevalent in the Chinese population ≥ 45 years with low back pain. More attention should be paid to the individuals at high-risk confirmed by this study to facilitate early identification and intervention against depressive symptoms.