bluuebunny/crossref_metadata_2025_split huggingface dataset:
A 29‐gene signature associated with NOX2 discriminates acute myeloid leukemia prognosis and survival
Authors: Ijurko Carla , González‐García Nerea , Galindo‐Villardón Purificación , Hernández‐Hernández Ángel 
Published: January 2022

AbstractThe molecular complexity displayed in acute myeloid leukemia (AML) hinders patient stratification and treatment decisions. Previous studies support the utility of using specific gene panels for this purpose. Focusing on two salient features of AML, the production of reactive oxygen species (ROS) by NADPH oxidases (NOX) and metabolism, we aimed to identify a gene panel that could improve patient stratification. A pairwise comparison of AML versus healthy gene expression revealed the downregulation of four members of the NOX2 complex including CYBB (coding for NOX2) in AML patients. We analyzed the expression of 941 genes related to metabolism and found 28 genes with expression correlated to CYBB. This panel of 29 genes (29G) effectively divides AML samples according to their prognostic group. The robustness of 29G was confirmed by 6 AML cohort datasets with a total of 1821 patients (overall accuracies of 85%, 78%, 80%, 75%, 59% and 83%). An expression index (EI) was developed according to the expression of the selected discriminatory genes. Overall Survival (OS) was higher for low 29G expression index patients than for the high 29G expression index group, which was confirmed in three different datasets with a total of 1069 patients. Moreover, 29G can dissect intermediate‐prognosis patients in four clusters with different OS, which could improve the current AML stratification scheme. In summary, we have found a gene signature (29G) that can be used for AML classification and for OS prediction. Our results confirm NOX and metabolism as suitable therapeutic targets in AML.
Outcome of Progressive Multifocal Leukoencephalopathy Treated by Interleukin‐7
Authors: Lajaunie Rébecca , Mainardi Ilaria , Gasnault Jacques , Rousseau Vanessa , Tarantino Andrea G. , Sommet Agnès , Cinque Paola , Martin‐Blondel Guillaume ,   PML study group
Published: January 2022

ObjectiveRestoring anti‐JC virus (JCV) immunity is the only treatment of progressive multifocal leukoencephalopathy (PML). Interleukin‐7 is a cytokine that increases number and function of T cells. We analyzed a population of PML patients who received recombinant human IL‐7 (rhIL‐7) to estimate survival and its determinants.MethodsAfter exclusion of patients with missing data or receiving other immunotherapies, findings from 64 patients with proven PML who received rhIL‐7 between 2007 and 2020 were retrospectively analyzed. Logistic regression was used to analyze variables associated with one‐year survival.ResultsUnderlying conditions were HIV/AIDS (n = 27, 42%), hematological malignancies (n = 16, 25%), primary immunodeficiencies (n = 13, 20%), solid organ transplantation (n = 4, 6%) and chronic inflammatory diseases (n = 4, 6%). One‐year survival was 54.7% and did not differ by underlying condition. Survival was not associated with baseline characteristics, but with a >50% increase in blood lymphocytes (OR 4.1, 95%CI 1.2–14.9) and CD4+ T cells (OR 5.9, 95%CI 1.7–23.3), and a > 1 log copies/mL decrease in cerebrospinal fluid JCV DNA (OR 7.6, 95%CI 1.6–56.1) during the first month after rhIL‐7 initiation. Side effects were mainly local and flu‐like symptoms (n = 8, 12.5%) and PML‐immune reconstitution inflammatory syndrome (IRIS) (n = 5, 8%).InterpretationIn this non‐controlled retrospective study, survival did not differ from that expected in HIV/AIDS patients, but might have been improved in those with hematological malignancies, primary immunodeficiencies and transplant recipients. RhIL‐7 might have contributed to the increase in blood lymphocytes and decrease in CSF JCV replication that were associated with better survival. ANN NEUROL 2022;91:496–505
Mechanismus der bifunktionellen Multiprodukt‐Sesterterpensynthase AcAS aus Aspergillus calidoustus
Authors: Quan Zhiyang , Hou Anwei , Goldfuss Bernd , Dickschat Jeroen S. 
Published: January 2022

AbstractDie chimäre Multiprodukt‐Sesterterpensynthase AcAS aus Aspergillus calidoustus lieferte neben fünf bekannten Sesterterpenen das spirocyclische Calidousten, welches ein neues Gerüst aufweist. Der komplexe Cyclisierungsmechanismus zu allen sechs Verbindungen wurde durch Isotopenmarkierungsexperimente in Kombination mit DFT‐Rechnungen untersucht. Chemisch synthetisiertes 8‐Hydroxyfarnesyldiphosphat wurde mit Isopentenyldiphosphat und AcAS in vier oxygenierte Sesterterpenoide umgewandelt, die strukturell den Cytochrom‐P450‐Oxidationsprodukten der Sesterterpen‐Kohlenwasserstoffe ähneln. Das Proteinengineering von AcAS erweiterte dessen Substratspektrum und führte zu signifikant verbesserten Enzymausbeuten.
Unmasking the Critical Role of the Ordering Degree of Bimetallic Nanocatalysts on Oxygen Reduction Reaction by In Situ Raman Spectroscopy
Authors: Chen Heng‐Quan , Ze Huajie , Yue Mu‐Fei , Wei Di‐Ye , A Yao‐Lin , Wu Yuan‐Fei , Dong Jin‐Chao , Zhang Yue‐Jiao , Zhang Hua , Tian Zhong‐Qun , Li Jian‐Feng 
Published: January 2022

AbstractPrecise control and accurate understanding of the ordering degree of bimetallic nanocatalysts (BNs) are challenging yet crucial to acquire advanced materials for the oxygen reduction reaction (ORR). AuCu BNs with various ordering degrees were synthesized to evaluate the influence of ordering degree on the ORR at a molecular level using in situ Raman spectroscopy. The activity of AuCu BNs was improved by over 2 times after a disorder‐to‐order transition, making the performance of highly ordered AuCu BNs exceed that of benchmark Pt/C. Direct Raman spectroscopic evidence of key intermediate (*OH) demonstrates that the active site is the combination site of Au and Cu. Moreover, two distinct *OH species are observed on the ordered and disordered structure, and the ordered site is more beneficial for ORR due to its lower affinity to *OH. This work deepens the understanding on the important role of ordering degree on BNs and enables the design of improved catalysts.
The synthesis of a copper metal‐organic framework Cu3TDPAT and its application in a Morita‐Baylis‐Hillman (MBH) reaction
Authors: Yang Yanan , Shang Hailing , Li Xiujuan , Zhu Kaicheng , Luan Yi 
Published: January 2022

AbstractA highly effective Morita‐Baylis‐Hillman (MBH) reaction was developed employing Cu (II) derived Cu3TDPAT as the dual‐functional metal‐organic frameworks (MOFs) catalyst. Four kinds of copper salts were employed as precursors to synthesize Cu3TDPAT. The resultant Cu3TDPAT MOFs were used as catalysts for the MBH model reaction of 4‐fluorobenzaldehyde and 2‐cyclopentene‐1‐one under various reaction conditions. The results showed that Cu3TDPAT‐c derived from copper nitrate was the optimal catalyst to achieve a high yield (up to 99%). With the presence of aromatic aldehydes and basic triazine groups in Cu3TDPAT, the Lewis acid and base sites in Cu3TDPAT MOF played a significant role during the reaction to effect the formation of CC single bond between 4‐fluorobenzaldehyde and 2‐cyclopentene‐1‐one to generate benzyl methyl acrylonitrile products. In addition, after optimization, this catalyst showed good catalytic performance for the MBH reaction with high thermal stability, reliability, and recyclability. This optimal catalytic performance shows great potential for further development.
Switchable activity of a Ru catalyst bearing an annulated mesoionic carbene ligand for oxidation of primary amines
Authors: Yadav Suman , Pal Saikat , Pal Nilay Kumar , Din Reshi Noor U , Pal Sourav , Bera Jitendra K. 
Published: January 2022

AbstractThe catalytic activity of a Ru complex 1, bearing a fused π‐conjugated imidazo[1,2–a][1,8]naphthyridine‐based mesoionic carbene (MIC) ligand, is examined for the oxidation of primary amines. Complex 1 affords nitrile or imine depending on the nature of the terminal oxidants and solvents used in the reactions. Primary amines are converted to nitriles using NaIO4 in EtOAc/H2O mixture, whereas imines are obtained under O2 balloon pressure in toluene. A variety of nitriles and imines are accessed with high yields and selectivity. A set of control experiments, reaction profiles, and kinetic studies are undertaken to disclose the mechanistic details for nitrile and imine formation. The catalytic reactions illustrate a subtle dependency on the choice of oxidants and solvents in the oxidation of primary amines.
Diluted High‐Concentration Electrolyte Based on Phosphate for High‐Performance Lithium‐Metal Batteries
Authors: Liu Mengchuang , Li Xing , Zhai Baoyu , Zeng Ziqi , Hu Wei , Lei Sheng , Zhang Han , Cheng Shijie , Xie Jia 
Published: January 2022

AbstractLithium (Li) metal is an ultra‐high specific capacity and low potential ‘‘holy grail’’ anode for developing high energy density rechargeable Li metal batteries (LMBs), but its practical application suffers from the low Coulombic efficiency (CE) and dendritic lithium growth due to its thermodynamic instability to organic solvents. Here, we report a new diluted high concentration electrolyte (DHCE) containing lithium bis(fluorosulfonyl)imide, triethyl phosphate and fluorobenzene cosolvent, which not only guarantees lithium metal anode (LMA) with an 98.4 % high average CE over 250 cycles but greatly improves Li||NMC622 cells property (70 % capacity retention after 200 cycles at C/3 current density). Therefore, this new electrolyte can provide more possibilities for developing high‐energy‐density LMBs.
Hot electrons generated from Mn‐doped quantum dots via upconversion for photocatalysis applications
Authors: Wang Chih‐Wei , Orrison Connor , Son Dong Hee 
Published: January 2022

AbstractThere is a growing interest in the application of photogenerated hot electrons in semiconductor or metallic nanostructures such as in photocatalysis and photovoltaics taking advantage of their large excess kinetic energy and long‐range transfer capability. Among various nanostructures that can generate hot electrons, Mn‐doped semiconductors quantum dots (QDs) have shown their unique ability to produce highly energetic hot electrons via upconversion process. Compared to the plasmonic hot electrons, upconverted hot electrons in Mn‐doped QDs possess several eV larger energy and can even produce photoelectron emission above the vacuum level from a subpopulation of hot electrons under weak visible excitation equivalent to the concentrated solar radiation. The present account reviews recent progresses made in the research on hot electron generation via upconversion and their application in photocatalytic reactions that highlights the benefits of long‐range transfer of energetic hot electrons.
Preparation of Porous Zn0.76Co0.24S Yolk‐Shell Microspheres with Enhanced Electrochemical Performance for Sodium Ion Batteries
Authors: Li Zuze , Zheng Yang , Li Qun , Gu Tingting , Shen Xueran , Ma Tiantian , Jiao Qingze , Zhao Yun , Li Hanshen , Feng Caihong 
Published: January 2022

AbstractTransition metal sulfides, due to excellent redox reversibility and considerable electronic conductivity, have been proposed as an available alternative material for rechargeable batteries. In this work, porous Zn0.76Co0.24S (ZCS) yolk‐shell microspheres are favorably obtained through a facile solvothermal manner, followed by a vulcanization process. The effects of vulcanization temperature on the morphology, structure, and electrochemical performance of the products are also explored. Owing to the unique buffering space and generous active sites of yolk‐shell structure, the electrode material exhibits excellent cycle and rate performance. Comparatively, Zn0.76Co0.24S vulcanized at 140 °C (ZCS‐140) retains a capacity of 671 mAh g−1 at 100 mA g−1 after 100 cycles and delivered a reversible capacity of 389 mAh g−1 at 1 A g−1 after 1000 cycles with a decay of only 0.029 % per cycle, which is better than that of ZCS‐120 and ZCS‐160, indicating its potential to be a promising next‐generation anode for SIBs.
Electrochemical Kinetics of Layered Manganese Phosphate via Interfacial Polypyrrole Chemical Binding
Authors: Du Jia‐Lin , Bao Xu , Zhang Wei‐Bin , Zhang Lun , Guo Yao‐Wen , Zhou Xia , Zhang Xian‐Li , Chai Shan‐Shan , Guo Shao‐Bo , Han Xiong‐Wei , Long Jianping 
Published: January 2022

AbstractThe major limitation of low‐cost and high‐safety aqueous manganese‐based materials for electrochemical energy storage is largely hampered by their poor cyclic stability, usually caused by the manganese dissolution and interfacial kinetics. Herein, we synthesized a manganese phosphate material via interfacial polypyrrole chemical binding, with unique structural properties for aqueous supercapacitor application. It exhibited higher specific capacitance, remarkable rate performance and better cycle stability compared to bare manganese phosphate. In contrast with interfacial polypyrrole coating on manganese phosphate nanosheets, the polypyrrole could form a Mn−N bond with the manganese phosphate, indicating stable chemical binding. Thus, the appropriate interfacial chemical binding is an efficient strategy to improve the nanostructure stability and electrochemical kinetics of manganese‐based materials for aqueous electrochemical energy storage.
Comparative enantioseparation of planar chiral ferrocenes on polysaccharide‐based chiral stationary phases
Authors: Dessì Alessandro , Sechi Barbara , Dallocchio Roberto , Chankvetadze Bezhan , Pérez‐Baeza Mireia , Cossu Sergio , Mamane Victor , Pale Patrick , Peluso Paola 
Published: January 2022

AbstractPlanar chiral ferrocenes are well‐known compounds that have attracted interest for application in synthesis, catalysis, material science, and medicinal chemistry for several decades. In spite of the fact that asymmetric synthesis procedures for obtaining enantiomerically enriched ferrocenes are available, sometimes, the accessible enantiomeric excess of the chiral products is unsatisfactory. In such cases and for resolution of racemic planar chiral ferrocenes, enantioselective high‐performance liquid chromatography (HPLC) on polysaccharide‐based chiral stationary phases (CSPs) has been used in quite a few literature articles. However, although moderate/high enantioselectivities have been obtained for planar chiral ferrocenes bearing polar substituents, the enantioseparation of derivatives containing halogens, or exclusively alkyl groups, remains rather challenging. In this study, the enantioseparation of ten planar chiral 1,2‐ and 1,3‐disubstituted ferrocenes was explored by using five polysaccharide‐based CSPs under multimodal elution conditions. Baseline enantioseparations were achieved for nine analytes with separation factors (α) ranging from 1.20 to 2.92. The presence of π‐extended systems in the analyte structure was shown to impact affinity of the most retained enantiomer toward amylose‐based selectors, observing retention times higher than 80 min with methanol‐containing mobile phases (MPs). Electrostatic potential (V) analysis and molecular dynamics (MD) simulations were used in order to study interaction modes at the molecular level.
Balancing Histone Deacetylase (HDAC) Inhibition and Drug‐likeness: Biological and Physicochemical Evaluation of Class I Selective HDAC Inhibitors
Authors: Schäker‐Hübner Linda , Haschemi Reza , Büch Thomas , Kraft Fabian B. , Brumme Birke , Schöler Andrea , Jenke Robert , Meiler Jens , Aigner Achim , Bendas Gerd , Hansen Finn K. 
Published: January 2022

AbstractHerein we report the structure‐activity and structure‐physicochemical property relationships of a series of class I selectiveortho‐aminoanilides targeting the “foot‐pocket” in HDAC1&2. To balance the structural benefits and the physicochemical disadvantages of these substances, we started with a set of HDACi related to tacedinaline (CI‐994) and evaluated their solubility, lipophilicity (log D7.4) and inhibition of selected HDAC isoforms. Subsequently, we selected the most promising “capless” HDACi and transferred its ZBG to our previously published scaffold featuring a peptoid‐based cap group. The resulting hit compound10 c(LSH‐A54)showed favorable physicochemical properties and is a potent, selective HDAC1/2 inhibitor. The following evaluation of its slow binding properties revealed thatLSH‐A54binds tightly to HDAC1 in an induced‐fit mechanism. The potent HDAC1/2 inhibitory properties were reflected by attenuated cell migration in a modified wound healing assay and reduced cell viability in a clonogenic survival assay in selected breast cancer cell lines.
Structurally Reinforced Silicon/Graphene Composite for Lithium‐Ion Battery Anodes: Carbon Anchor as a Conductive Structural Support
Authors: Hoon Park Byung , Yang Hanmo , Gil Choi Yong , Kim Kwang‐Bum 
Published: January 2022

AbstractHerein, a Si/reduced graphene oxide (rGO)/C microsphere composite is reported, wherein sucrose‐derived carbon binds Si nanoparticles (NPs) and rGO to act as a carbon anchor and links neighboring rGO sheets to reinforce the composite structure. In this structurally reinforced Si/rGO/C composite, the electron conduction pathways between rGO and Si NPs were maintained even under large volume changes during repeated charge–discharge processes. Consequently, the Si/rGO/C composite anode exhibited an initial discharge capacity of 1209 mAh g−1 and superior cyclability (92 % retention at 100 cycles), initial coulombic efficiency of 80.5 %, and high‐rate capability even at a high C rate (6 C). Furthermore, the change in anode thickness after repeated cycling was negligible, confirming the structural stability imparted by the sucrose‐derived carbon binder. A full cell assembled with a LiCoO2 cathode and the Si/rGO/C composite anode remained stable over 200 cycles.
Genome‐wide off‐target analyses of CRISPR/Cas9‐mediated T‐cell receptor engineering in primary human T cells
Authors: Kaeuferle Theresa , Stief Tanja A , Canzar Stefan , Kutlu Nayad N , Willier Semjon , Stenger Dana , Ferrada‐Ernst Paulina , Habjan Nicola , Peters Annika E , Busch Dirk H , Feuchtinger Tobias 
Published: January 2022

AbstractObjectivesExploiting the forces of human T cells for treatment has led to the current paradigm of emerging immunotherapy strategies. Genetic engineering of the T‐cell receptor (TCR) redirects specificity, ablates alloreactivity and brings significant progress and off‐the‐shelf options to emerging adoptive T‐cell transfer (ACT) approaches. Targeted CRISPR/Cas9‐mediated double‐strand breaks in the DNA enable knockout or knock‐in engineering.MethodsHere, we perform CRISPR/Cas9‐mediated TCR knockout using a therapeutically relevant ribonucleoprotein (RNP) delivery method to assess the safety of genetically engineered T‐cell products. Whole‐genome sequencing was performed to analyse whether CRISPR/Cas9‐mediated DNA double‐strand break at the TCR locus is associated with off‐target events in human primary T cells.ResultsTCRα chain and TCRβ chain knockout leads to high on‐target InDel frequency and functional knockout. None of the predicted off‐target sites could be confirmed experimentally, whereas whole‐genome sequencing and manual Integrative Genomics Viewer (IGV) review revealed 9 potential low‐frequency off‐target events genome‐wide. Subsequent amplification and targeted deep sequencing in 7 of 7 evaluable loci did not confirm these low‐frequency InDels. Therefore, off‐target events are unlikely to be caused by the CRISPR/Cas9 engineering.ConclusionThe combinatorial approach of whole‐genome sequencing and targeted deep sequencing confirmed highly specific genetic engineering using CRISPR/Cas9‐mediated TCR knockout without potentially harmful exonic off‐target effects.
LncRNA LINC00942 promotes chemoresistance in gastric cancer by suppressing MSI2 degradation to enhance c‐Myc mRNA stability
Authors: Zhu Yiran , Zhou Bingluo , Hu Xinyang , Ying Shilong , Zhou Qiyin , Xu Wenxia , Feng Lifeng , Hou Tianlun , Wang Xian , Zhu Liyuan , Jin Hongchuan 
Published: January 2022

AbstractBackgroundChemoresistance to cisplatin (DDP) remains a major challenge in advanced gastric cancer (GC) treatment. Although accumulating evidence suggests an association between dysregulation of long non‐coding RNAs (lncRNAs) and chemoresistance, the regulatory functions and complexities of lncRNAs in modulating DDP‐based chemotherapy in GC remain under‐investigated. This study was designed to explore the critical chemoresistance‐related lncRNAs in GC and identify novel therapeutic targets for patients with chemoresistant GC.MethodsChemoresistance‐related lncRNAs were identified through microarray and verified through a quantitative real‐time polymerase chain reaction (qRT‐PCR). Proteins bound by lncRNAs were identified through a human proteome array and validated through RNA immunoprecipitation (RIP) and RNA pull‐down assays. Co‐immunoprecipitation and ubiquitination assays were performed to explore the molecular mechanisms of the Musashi2 (MSI2) post‐modification. The effects of LINC00942 (LNC942) and MSI2 on DDP‐based chemotherapy were investigated through MTS, apoptosis assays and xenograft tumour formation in vivo.ResultsLNC942 was found to be up‐regulated in chemoresistant GC cells, and its high expression was positively correlated with the poor prognosis of patients with GC. Functional studies indicated that LNC942 confers chemoresistance to GC cells by impairing apoptosis and inducing stemness. Mechanically, LNC942 up‐regulated the MSI2 expression by preventing its interaction with SCFβ‐TRCP E3 ubiquitin ligase, eventually inhibiting ubiquitination. Then, LNC942 stabilized c‐Myc mRNA in an N6‐methyladenosine (m6A)‐dependent manner. As a potential m6A recognition protein, MSI2 stabilized c‐Myc mRNA with m6A modifications. Moreover, inhibition of the LNC942‐MSI2‐c‐Myc axis was found to restore chemosensitivity both in vitro and in vivo.ConclusionsThese results uncover a chemoresistant accelerating function of LNC942 in GC, and disrupting the LNC942‐MSI2‐c‐Myc axis could be a novel therapeutic strategy for GC patients undergoing chemoresistance.
The Effects of Frying Method and Oil Type on the Quality Attributes of Turkey Nuggets and Frying Oils
Authors: Şişik Oğraş Şeyma , Kaplan Hatice 
Published: January 2022

AbstractThe aim of the study is to determine the effects of frying under standard atmospheric and pressure conditions on some quality characteristics of turkey nuggets. Turkey nuggets are fried under atmospheric and pressure conditions using sunflower or hazelnut oil. Pressure frying decreases the thiobarbituric acid reactive substances (TBARS) value of turkey nuggets while sunflower oil increases this value. Under atmospheric conditions, nuggets fried with hazelnut oils show higher moisture content than hazelnut oil. The pH is affected by the type of oil. The frying under pressure results in a decrease in the internal and external a* value of nuggets. Under pressure conditions, the use of hazelnut oil increases the external a* value. The application of pressure also causes an increase in the external L* value in the presence of sunflower oil. These treatments also show higher hardness, springiness, gumminess, and chewiness values compared to atmospheric condition. The use of sunflower oil causes a decrease in resilience, cohesiveness, springiness, and chewiness values. After frying, hazelnut oil shows lower peroxide and total polar material values than sunflower oil. However, peroxide value and total polar material are not affected by frying method.Practical applications: Color and texture are very important factors for the quality of nuggets. Pressure and atmospheric conditions show different effects on color parameters. Frying under pressure conditions decreases the external L*, which is an important quality parameter. Hazelnut oil shows lower TBARS value than sunflower oil. Moreover, pressure application causes a decrease in TBARS value. Frying process under pressure decreases the hardness and adhesiveness, while it increases the juiciness of product. On the other hand, the use of hazelnut oil as frying oil shows less changes in peroxide and total polar material during frying than sunflower oil, but the frying method and oil type interaction has no significant influence on these parameters.