bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between serum homocysteine and Vitamin D with positive, negative and extrapyramidal symptom domain in Schizophrenia: Case-control study in Iran
Authors: shahini najmeh , Jazayeri Seyed Mohammad Mousavi Zade , jahanshahi Reza , Charkazi Abdurrahman 
Published: June 2022

Abstract
Background: Schizophrenia is a devastating condition characterized by recurrent recurrences, cognitive decline, emotional and functional disabilities. This condition includes positive and negative symptoms and cognitive impairment that are very resistant to drug treatment. According to studies, many biomarkers can affect this disorder. However, there is little information about vitamin D and homocysteine levels in patients with disease complications. In the current study, we aimed to investigate this relationship in schizophreniaMethod: This is a case-control study. Thirty-three patients with schizophrenia and 33 healthy individuals from north of Iran-Golestan in 2021 were analyzed. Blood samples were taken from all participants to assess serum levels of vitamin D and homocysteine. In addition, schizophrenic patients completed the Positive and Negative Syndrome Scale (PANSS) and Simpson-Angus Extrapyramidal Side Effects Scale (SAS) questionnaires. Data analysis was performed at a significance level of 0.05 using SPSS 16 software.Results: Of the 66 participants, 66.7% had vitamin D deficiency, and 71.2% had normal homocysteine levels. However, the serum level of vitamin D in schizophrenic patients was lower than in controls (p= 0.035) and serum homocysteine ​​levels were higher in the schizophrenic group than in the control group (p<0.001). Vitamin D levels in patients with schizophrenia were significantly correlated with the overall assessment of extrapyramidal symptoms (r= 0.35, p=0.04). The data obtained did not show a significant relationship between vitamin D and homocysteine ​​levels and PANSS test results (p= 0.3 and 0.8).Conclusion: Serum levels of vitamin D and homocysteine in schizophrenic patients were significantly lower and higher than in the control group, respectively. Improvement of extrapyramidal symptoms in schizophrenic patients has a direct and significant relationship with serum vitamin D.
Simulation of thermal compression and texture evolution of TB6 titanium alloy
Authors: EnRui Zhu , Lulu Guo , Xia Cui , DeLai OuYang 
Published: June 2022

Abstract
The isothermal constant strain rate compression experiment of TB6 titanium alloy was carried out by a Thermecmaster-Z thermal simulator and the stress-strain curves under corresponding conditions was analyzed and discussed. The thermal compression process was simulated by finite element simulation software, and the finite element software was redeveloped to simulate the ODFs of this alloy after experimental at the same time. The results showed that the stress-strain curves by numerical simulation were consistent with the experimental results. The distribution of equivalent stress and strain was uneven, the maximum stress and strain occurred at the center of the specimens. The stress and strain increased with the decrease of deformation temperature or the increase of strain rate. The simulated ODFs had a high agreement with the ODFs by EBSD technology. After thermal compression, the major texture components of the alloy were R-CubeND {001}<110 > and Cube {001}<100 > and they had certain heredity. Increasing the deformation temperature or strain rate can intensify the major texture components and promoted the transition from Y {111}<112 > texture component to Z {111}<110 > texture component. Meanwhile, the intensity of S {123}<634>, Copper {112}<111>, Y {111}<112 > and Z {111}<110 > texture components were reduced.
Biochemical characterization of local populations of Physalis minima suggests correlation between its fruit and medicinal properties
Authors: ANJANI KUMARI , SHARMA V. K. , KUMAR HARSH 
Published: June 2022

Abstract
Physalis minima L. is known to possess numerous medicinal properties and has a potential to be utilized as a fruit plant, but environment induced variations in biochemical constitution have not been identified. The present study was thus carried out for biochemical characterization of different populations of Physalis minima plants of Bihar and determine the correlation between medicinal and fruit value of the plant. Nineteen quantitative biochemical parameters were recorded for the plants of seven populations. Substantial variations within and between populations for all the biochemical characters was observed. The findings indicated the presence of positive and negative correlation within and between the nutritive and medicinal parameters of the plants. The biochemical characters were successfully able to distinguish the populations into different groups. The study thus concludes that the P. minima plants found to have high medicinal value will also have highly nutritious fruits and the biochemical characters can be used as markers to characterize plants of different regions and determine the relatedness between them. The information will enable easy selection of the commercially beneficial plants.
Efficacy and safety of pembrolizumab monotherapy for recurrent/unresectable/metastatic oral squamous cell carcinoma: A single-centre study in China
Authors: Li Jieying , He Zongxuan , Tao Yueqin , Yang Xiaochen , Ge Shengyou , Shang Wei , Song Kai 
Published: June 2022

Abstract
Background
Although pembrolizumab can be recommended as a first-line medicine for the treatment of advanced recurrent/unresectable/metastatic(R/U/M)head and neck squamous carcinoma, the differences in efficacy of pembrolizumab in different populations needs to be further investigated.
Methods
We reviewed 15 consecutive patients with R/U/M oral squamous cell carcinoma (OSCC) who were treated with pembrolizumab monotherapy at the Affiliated Hospital of Qingdao University between February 2021 and May 2022. All 15 patients with known programmed death-ligand 1 expression received multiple cycles of pembrolizumab monotherapy as first-line treatment. The basic characteristics of patients, time to first remission, clinical efficacy, and related adverse reactions were evaluated and analysed.
Results
The objective response rate of the 15 patients was 60%. Six patients (40.0%) achieved partial response and three (20.0%) achieved complete response. The status of objective response in patients can be observed in two to five cycles (mean, 3.6 cycles) in our study. For patients who responded well to immunotherapy, the mean Karnofsky Performance Status (KPS) score after treatment was significantly higher than the KPS score before treatment (P༜0.001). The progression-free survival rates were 66.9% and 50.1% at 6 months and 1 year, respectively. Eight adverse events were observed in this study: four rash, one hypothyroidism, one interstitial pneumonia, one cheilitis and one cerebral thrombosis.
Conclusion
Our study suggests that pembrolizumab is beneficial for the most responsive patients with R/U/M OSCC in our single-centre study and may shed light on the management of OSCC.
Brain metastases in Japanese NSCLC patients: prognostic assessment and the use of osimertinib and immune checkpoint inhibitors: a retrospective study
Authors: Higaki Hajime , Nishioka Kentaro , Otsuka Manami , Nishikawa Noboru , Shido Motoyasu , Minatogawa Hideki , Nishikawa Yukiko , Takashina Rikiya , Hashimoto Takayuki , Katoh Norio , Taguchi Hiroshi , Kinoshita Rumiko , Yasuda Koichi , Mori Takashi , Uchinami Yusuke , Koizumi Fuki , Fujita Yoshihiro , Takahashi Shuhei , Hattori Takahiro , Nishiyama Noriaki , Aoyama Hidefumi 
Published: June 2022

Abstract
Background: The Graded Prognostic Assessment for lung cancer using molecular markers (Lung-molGPA) has not yet been validated for use with Japanese non-small cell lung cancer (NSCLC) patients with brain metastasis (BM) and the factors impacting survival need to be assessed.Methods: We retrospectively analyzed 294 NSCLC patients who were newly diagnosed with BM between 2013 and 2020 and received radiotherapy for BM initially at the Hokkaido Cancer Center. Lung-molGPA items, sequence of therapy, use of osimertinib, expression of PD-L1 (classified as high, low, and no) and use of immune checkpoint inhibitors (ICI) were evaluated for influence. The main outcome measure was survival from the day of diagnosis of BM, and log-rank tests were performed to compare the results.Results: The median survival times for adenocarcinoma by groupings of GPA score (0‒1.0, 1.5‒2.0, 2.5‒3.0, and 3.5‒4.0) were 5.5, 14.8, 28.3, and 39.0 months (p<0.0001), respectively. The median survival times for non-adenocarcinoma by groupings of GPA score (0‒1.0, 1.5‒2.0, and 2.5‒3.0) Group were 3.2, 11.0, and 16.0 months (p=0.0011), respectively. In adenocarcinoma patients with gene mutations, osimertinib significantly improved the outcome. The median survival times for the group with and without osimertinib treatment were 34.2 and 17.6 months, respectively (p=0.0164). In a comparison of treatment sequences, no significant differences were found between groups; however, patients who received both irradiation and tyrosine-kinase inhibitor for BM had better prognoses than patients with only irradiation for BM (35.8 months vs 14.8 months, p=0.0007). In patients tested for PD-L1 expression, the median survival times after diagnosis of brain metastasis were 5.6, 22.5, and 9.3 months for high-, low- and no-expression groups (p=0.2198), respectively. Also, in patients with high PD-L1 expression, those with ICI had better survival (median OS, 8.6 months) than those without (median OS, 3.6 months).Conclusions: We conformed that Lung-molGPA successfully classified Japanese NSCLC patients with brain metastasis by prognosis. Osimertinib was shown to extend survival of EGFR-positive NSCLC patients with BM, and ICI proved effective in patients with high PD-L1 expression.Trial registration: Not applicable
Effect of activities of daily living disability on the hospitalization of middle-aged and elderly people in China: a cross-sectional study based on CHARLS data
Authors: Jian Yang , Yuan Yang , Lei Tang , Ruoyun Yin , Qian Hu , Cong Cheng , Fan Zhang 
Published: June 2022

Abstract
BackgroundActivities of daily living (ADL) has been an enduring topic in public health. This study aimed to investigate the relationship of ADL and hospitalization among middle-aged and elderly people, and discusses ADL into two aspects.MethodsWe used baseline data from China Health and Retirement Longitudinal Study (CHARLS). A total of 17,969 subjects was included in this study. The basic activities of daily living (BADL) and the instrumental activities of daily living (IADL) were evaluated by the Activities of Daily Living Scale (ADLS) and Lawton Functional Scale. Stata16.0 and SPSS26.0 were used for data processing and statistical analysis respectively. The chi-square test was used for univariate analysis of hospitalization risk, and the logistic regression was used for the multivariate analysis. ResultsAfter controlling for confounders, the hospitalization risk was higher with ADL disability than with no ADL disability (OR=1.413, P<0.01). In order to further understand the influence of ADL types on depression, BADL and IADL were included in the multivariate analysis and found that BADL disability (OR=1.256, P<0.01) and IADL disability (OR=1.375, P<0.01) were associated with the hospitalization risk. Gender, age, household registration type, social health insurance, self-rated health level, the number of chronic diseases, smoking and alcohol drinking also have a relatively great impact on the hospitalization of middle-aged and elderly people (P<0.001).ConclusionsBADL and IADL disability were the risk factors of hospitalization in middle-aged and elderly patients. It was necessary to deeply reform our social medical security system to promote the health of people with ADL disability.
Palliative Care Team in a Brazilian Tertiary Emergency Department
Authors: Lourençato Frederica Montanari , Miranda Carlos Henrique , Borges Marcos de Carvalho , Pazin-Filho Antonio 
Published: June 2022

Abstract
Objectives: To describe the process of implementing a palliative care team (PCT) in a Brazilian public tertiary university hospital and compare this intervention as an active in-hospital search (Strategy I) with the Emergency Department (Strategy II). Methods: We described the development of a complex Palliative Care Team (PCT). We evaluated the following primary outcomes - hospital discharge, death (in-hospital and follow-up mortality) or transfer - and performance outcomes - Perception Index (difference in days between hospitalization and the evaluation by the PTC), follow-up index (difference in days between the PTC evaluation and the primary outcome), and the in-hospital stay. Results: We included 1203 patients - Strategy I (587; 48.8%) and Strategy II (616; 51.2%). In both Strategies, male and elderly patients were prevalent. Most came from internal medicine I (39.3%) and II (57.9%) - p<0.01. General clinical conditions (40%) and Oncology - I (27.7%) and II (32.4%) - represented the majority of the population. Over 70% of all patients had PPS 10 and ECOG 4 above 85%. There was a reduction of patients identified in ICU from I (20.9%) to II (9.2%) - p <0.01, reduction in the ward from I (60.8%) to II (42.5%) - p <0.01 - and a significant increase from I (18.2%) to II (48.2%) in the emergency department - p <0.01. Regarding in-hospital mortality, 50% of patients remained alive within 35 days of hospitalization (Strategy I), while for Strategy II, 50% were alive within 20 days of hospitalization (p<0 .01). As for post-discharge mortality, in Strategy II, 50% of patients died ten days after hospital discharge, while in Strategy I, this number was 40 days (p<0.01). In the Cox multivariate regression model, adjusting for possible confounding factors, Strategy II increased 30% the chance of death. The perception index decreased from 10.9 days to 9.1 days, there was no change in follow-up (12 days), and the duration of in-hospital stay dropped from 24.3 to 20.7 days - p <0.01. The primary demand was the definition of prognosis (56.7%). Conclusion: The present work showed that early intervention by an elaborate and complex PCT in the ED was associated with a faster perception of the need for palliative care and influenced a reduction in the length of hospital stay in a very dependent and compromised old population.
Analysis of lncRNA-associated ceRNA network in breast cancer: A new insight into the role of GBP1P1 pseudogene in breast cancer
Authors: Mohebifer Hossein , Sabaghian Amir , Kalani Mohamad Reza , Farazmandfar Touraj , Golalipour Masoud 
Published: June 2022

Abstract
Background: Pseudogenes are a type of long noncoding RNAs (lncRNA) demonstrated to have a remarkable ability in post-transcriptional regulation, including cancer. Pseudogenes regulate parental gene expression via the competing endogenous RNA (ceRNA) mechanism. In this study, we present an lncRNA ceRNA network in breast cancer.Methods: 378 samples from 10 GEO databases were used to search for significant differentially expressed genes and lncRNAs. Following, the results were confirmed in the TCGA breast cancer database. The experimentally validated miRNAs that target genes and lncRNAs were explored in the DIANA tool to construct a ceRNA network. Finally, the result of ceRNA network analysis was validated in 40 breast cancer patients in lab experiments.Results: We have found 962 genes and lncRNAs in breast cancer. A ceRNA network with the topmost correlated gene-lncRNA and 73 common miRNAs was constructed. Several cancer-related pathways were deregulated, including AKT/mTOR and mTORC1-mediated pathways. We have found three genes with pseudogene pairs with the highest correlations, including GBP1-GBP1P1, PDE4DIP- PDE4DIPP2, and CD24-CD24P4. The GBP1-GBP1P1 had the best correlation with age, menopause status, nodal metastasis, stage, and subclass of patients. Then we assessed the expression of GBP1 and GBP1P1 in 40 breast cancer patients. The expression levels of GBP1 and its pseudogene were significantly upregulated in tumor samples (3.4 and 5.7 fold, respectively). In conclusion, we report a gene-lncRNA ceRNA network in breast cancer that may provide new insights into the role of GBP1P1 in the development of breast cancer.
Highly efficient removal of arsenate and arsenite with potassium ferrate: Role of in situ formed ferric nanoparticle
Authors: Kong Yanli , Ma Yaqian , Guo Meng , Huang Zhiyan , Ma Jiangya , Nie Yong , Ding Lei , Chen Zhonglin , Shen Jimin 
Published: June 2022

Abstract
It is well known that the capacity of potassium ferrate (Fe(VI)) for the oxidation of pollutants or co-precipitation and adsorption of hazardous species. However, little information has been paid on the adsorption and co-precipitation contribution of the Fe(VI) resultant nanoparticles, the in-situ hydrolytic ferric iron oxides. Here, the removal of arsenate (As(V)) and arsenite (As(III)) by Fe(VI) was investigated, which focused on the interaction mechanisms of Fe(VI) with arsenic, especially in the contribution of the co-precipitation and adsorption of its hydrolytic ferric iron oxides. pH and Fe(VI) played significant roles on arsenic removal, over 97.8% and 98.1% of As(V) and As(III) removal were observed when Fe(VI) : As(V) and Fe(VI) : As(III) were 24 : 1 and 16 : 1 at pH 4, respectively. The removal of As(V) and As(III) by in-situ and ex-situ formed hydrolytic ferric iron oxides was examined respectively. The results revealed that As(III) was oxidized by Fe(VI) to As(V), and then was removed though co-precipitation and adsorption by the hydrolytic ferric iron oxides with the contribution content was about 1 : 3. While for As(V), it could be removed directly by the in situ formed particles from Fe(VI) through co-precipitation and adsorption with the contribution content was about 1 : 1.5. By comparison, As(III) and As(V) were mainly removed through adsorption by the 30 min hydrolytic ferric iron oxides during the ex-situ process. The hydrolytic ferric iron oxides size was obviously different in the process of in-situ and ex-situ, possessing abundant and multiple morphological structures ferric oxides, which was conducive for the efficient removal of arsenic. This study would provide a new perspective for understanding the potential of Fe(VI) treatment on arsenic control.
A Bibliometric Analysis of coronary microcirculation over a 30-Year Period
Authors: Ling Hao , Fu Sunjing , Wang Bing , Li Yuan , Li Bingwei , Wang Qin , Liu Xueting , Zhang Xiaoyan , Li Ailing , LIU Mingming 
Published: June 2022

Abstract
Coronary microcirculation has a fundamental role in the regulation of coronary blood flow in response to cardiac requirements, which has aroused wide concerns in basic science and clinical cardiovascular research. A comprehensive understanding of the development status of this field is conducive to its improvement. We aimed to analyze coronary microcirculation-associated literatures over 30 years and provide insightful information on the evolutionary path, frontier research hotspots, and future developmental trends. Publications were retrieved from the Web of Science Core Collection (WoSCC). VOSviewer was used to perform co-occurrence analyses for countries/regions, institutions, and authors and to generate visualized collaboration network maps. CiteSpace was used to analyze the raw data and to visualize the knowledge map derived from reference co-citation analysis, burst references, and keywords detection. The collected 11,718 publications showed an annually increasing trend. The United States and Harvard University ranked at the top among all the countries and institutions. The majority of articles were published in Circulation, and it also was the most co-cited journal. Co-citation analysis, burst detection, and alluvial flow map revealed that thematic hotspots and frontiers on coronary microcirculation were focused on coronary microvascular dysfunction, magnetic resonance imaging, fractional flow reserve, STEMI, and heart failure. Additionally, keywords burst showed that the prognostic value and management were current knowledge gaps and future directions. Coronary microcirculation presented a research hotspot relevant wide spectrum of clinical cardiovascular diseases. The definite diagnostics and prognostics are particularly valued. The protection of cardiovascular events that influence clinical outcomes should be an insightful concern in the future. Multidisciplinary collaborations will provide significant advances for the development of coronary microcirculation.
GCAF(TMEM251) regulates lysosome biogenesis by activating the mannose-6-phosphate pathway
Authors: Zhang Weichao , Yang Xi , Li Yingxiang , Yu Linchen , Zhang Bokai , Zhang Jianchao , Cho Woo Jung , Venkatarangan Varsha , Chen Liang , Burugula Bala Bharathi , Bui Sarah , Wang Yanzhuang , Duan Cunming , Kitzman Jacob , li Ming 
Published: June 2022

Abstract
The mannose-6-phosphate (M6P) biosynthetic pathway for lysosome biogenesis has been studied for decades and is considered a well-understood topic. However, whether this pathway is regulated remains an open question. In a genome-wide CRISPR/Cas9 knockout screen, we discovered TMEM251 as the first regulator of the M6P modification. Deleting TMEM251 caused mistargeting of most lysosomal enzymes due to their loss of M6P modification and accumulation of numerous undigested materials. We further demonstrated that TMEM251 localizes to the Golgi and is required for the cleavage and activation of GNPT, the enzyme that catalyzes M6P modification. In zebrafish, TMEM251 deletion leads to severe developmental defects including heart edema and skeletal dysplasia, which phenocopies Mucolipidosis Type II. Our discovery provides a mechanism for the newly discovered human disease caused by TMEM251 mutations. We name TMEM251 as GNPTAB cleavage and activation factor (GCAF) and its related disease as Mucolipidosis Type V.
The glycolysis inhibitor 2-deoxy-D-glucose exerts different neuronal effects at circuit and cellular levels, partially reverses behavioral alterations and does not prevent NADPH diaphorase activity reduction in the intrahippocampal kainic acid model of temporal lobe epilepsy
Authors: Khatibi Vahid Ahli , Rahdar Mona , Rezaei Mahmoud , Davoudi Shima , Nazari Milad , Mohammadi Mohammad , Raoufy Mohammad Reza , Mirnajafi-Zadeh Javad , Hosseinmardi Narges , Behzadi Gila , Janahmadi Mahyar 
Published: June 2022

Abstract
Temporal lobe epilepsy is the most drug-resistant type with the highest incidence among the other focal epilepsies. Metabolic manipulations are of great interest among others, glycolysis inhibitors like 2-deoxy d-glucose (2-DG) being the most promising intervention. Here, we sought to investigate the effects of 2-DG treatment on cellular and circuit level electrophysiological properties using patch-clamp and local field potentials recordings and behavioral alterations such as depression, anxiety-like behaviors, and changes in nitric oxide signaling in the intrahippocampal kainic acid model. We found that epileptic animals were less anxious, more depressed, with more locomotion activity. Interestingly, by masking the effect of increased locomotor activity on the parameters of the zero-maze test, no altered anxiety-like behavior was noted in epileptic animals. However, 2-DG could partially reverse the behavioral changes induced by kainic acid. The findings also showed that 2-DG treatment partially suppresses cellular level alterations while failing to reverse circuit-level changes resulting from kainic acid injection. Analysis of NADPH-diaphorase positive neurons in the CA1 area of the hippocampus revealed that the number of positive neurons was significantly reduced in dorsal CA1 of the epileptic animals and 2-DG treatment did not affect the diminishing effect of kainic acid on NADPH-d+ neurons in the CA1 area. In the control group receiving 2-DG, however, an augmented NADPH-d+ cell number was noted. These data suggest that 2-DG cannot suppress epileptiform activity at the circuit-level in this model of epilepsy and therefore, may fail to control the seizures in temporal lobe epilepsy cases.