bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-Specific Effect on Accumulated Oxygen Deficit During Short and Middle-Distance Swimming Performance
Authors: Massini Danilo A. , Almeida Tiago A. F. , Macedo Anderson G. , Espada Mário A. C. , Reis Joana F. , Alves Francisco J. B. , Fernandes Ricardo J. P. , Filho Dalton M. Pessôa 
Published: March 2022

Abstract
This study assessed the accumulated oxygen deficit (AOD) during 50, 100, and 200 m front-crawl performances to compare the responses between sexes and analyse the effect of lean body mass (LBM). Twenty swimmers (16.2 ± 2.8 years, 61.6 ± 7.8 kg, and 48.8±11.2 kg LBM) performed 50, 100, and 200 m to determine accumulated oxygen uptake (V̇O2Ac). The swimmers also performed an incremental test from which five submaximal steps were selected to estimate the oxygen demand (V̇O2demand) from the V̇O2 vs velocity adjustment. V̇O2 was sampled using a gas analyser coupled with a respiratory snorkel. AOD was the difference between V̇O2demand and V̇O2Ac, and LBM was assessed by dual-energy absorptiometry. A two-way ANOVA evidenced an AOD increase with distance for both sexes: 19.7 ± 2.5 vs 24.9 ± 5.5, 29.8 ± 8.0 vs 36.5 ± 5.8, and 41.5 ± 9.4 vs 55.2 ± 11.9 ml×kg−1, respectively for 50, 100, and 200 m (with highest values for females, P < 0.01). Inverse correlations were observed between LBM and AOD for 50, 100, and 200 m (r = -0.60, -0.38 and − 0.49, P < 0.05). AOD increased from 50 to 200 m distances for both sexes. However, females presented higher AOD values, which probably relates to the effect of reduced LBM on increasing the rate of oxygen deficit (i.e., anaerobic energy demand).
Vibration monitoring and analysis of strip rolling mill based on the digital twin model
Authors: Zhang Yang , Wang Weizhong , Zhang Huan , Li Haichao , Liu Cuirong , Du Xiaozhong 
Published: March 2022

Abstract
This paper presents a monitoring method of strip rolling mill based on the digital twin model. Compared with the traditional monitoring methods, this method effectively reduces structure complexity and test cost of the monitoring system. In this paper, the coupling vibration mechanism model of the dynamic characteristics of roll system and bearings of four-high mill was established, and the digital twin model of strip rolling mill vibration was built based on the mechanism model. Consequently, the traditional monitoring test of the rolling mill was carried out to verify the accuracy of the digital twin model. Through analyzing the test results, it can be found that when the rolling force fluctuation is more obvious, the rolling mill roll system vibration becomes more intense. According to this phenomenon, the measures were put forward to suppress rolling mill vibration. In conclusion, the rolling mill vibration monitoring theory based on digital twin model provides technical support and theoretical basis for improving equipment operation stability, reducing test cost and improving product quality.
In vitro colonic fermentation of ultrasonicated blackberry (Rubus fruticosus) residues cv. Tupy
Authors: Zafra-Rojas Quinatzin Yadira , Cruz-Cansino Nelly del Socorro , Cervantes-Elizarrarás Alicia , Ariza-Ortega José Alberto , Hernández-Hernández Hilda María , Díaz-Batalla Luis , Olloqui Enrique J. , Ramírez-Moreno Esther 
Published: March 2022

Abstract
The objective of present study was evaluated non fermented residue (NFR), disappeared organic matter (DOM), bacteria acid lactic (LAB), short-chain fatty acids (SCFA), flavonoids and the microstructure during in vitro colonic fermentation of ultrasonicated blackberry residues (pulp, peel and seeds). Results showed that ultrasonicated blackberry residues (US-BR) presented low NFR (6.30 ± 1.27 mg) and a high percentage of DOM (93.70 ± 1.27%) in comparison with blackberry residues (BR), indicating high fermentability of the sample. The same behavior in the other parameters was observed, since had an increase in LAB (6.97 ± 0.08 log CFU/mL), total SCFA (409.49 mg/L), individual fatty acids such as acetic and propionic acid (387.65 ± 3.60 and 20.17 ± 0.15 mg/L, respectively). Also total flavonoids were high in US-BR (1.3 mg/100 g) where flavonoids myricetin and quercetin were identified. The microstructure of the fermented US-BR had a reduction of particle size and less fibrous matrix compared with the fermented BR. The use of ultrasound improves the properties of the fibrous matrix of blackberry residues available for intestinal microbiota, obtaining final products of fermentation with possible health benefits.
Bone marrow mesenchymal stem cells facilitate diabetic wound healing through the restoration of epidermal cell autophagy via the HIF-1α/TGF- β1 /SMAD pathway
Authors: Yang Ronghua , Shi Yan , Wang Shang , Zhang Weiwei , Zhu Yihan , Fan Zhiqiang , Huang Yuesheng , Li Furong 
Published: March 2022

Abstract
Background: The biological activity and regenerative medicine of bone marrow mesenchymal stem cells (BMSCs) have been focal topics in the broad fields of diabetic wound repair. However, the molecular mechanisms are still largely elusive for other cellular processes that are regulated during BMSC treatment. Our previous studies have shown that hypoxia is not only a typical pathological phenomenon of wounds but also exerts a vital regulatory effect on cellular bioactivity. In this study, the beneficial effects of hypoxia BMSCs on the cellular behaviors of epidermal cells and diabetic wound healing were investigated.Method: The viability and secretion ability of hypoxic BMSCs were detected. The autophagy, proliferation and migration of HaCaT cells cultured with hypoxic BMSCs-derived conditioned medium were assessed by estimating the expression of autophagy-related proteins, MTS, EdU proliferation and scratch assays. And the role of the SMAD signaling pathway during hypoxic BMSC-evoked HaCaT cell autophagy were explored through a series of in vitro gain- and loss-of-function experiments. Finally, the therapeutic effects of hypoxic BMSCs were evaluated using full-thickness cutaneous diabetic wound model.Results: First, we demonstrated that hypoxic conditions intensify HIF-1α-mediated TGF-β1 secretion by BMSCs. Then, the further data revealed that BMSC-derived TGF-β1 was responsible for the activation of epidermal cell autophagy, which contributed to the induction of epidermal cell proliferation and migration. Here, the SMAD signaling pathway was identified as downstream of BMSC-derived TGF-β1 to regulate HaCaT cell autophagy. Moreover, the administration of BMSCs to diabetic wounds increased epidermal autophagy and the rate of re-epithelialization, leading to accelerated healing, and these effects were significantly attenuated, accompanied by the downregulation of Smad2 phosphorylation levels due to TGF-β1 interference in BMSCs. Conclusion: In this report, we present evidence that uncovers a previously unidentified role of hypoxic BMSCs in regulating epidermal cell autophagy. The findings demonstrate that BMSC-based treatment by restoring epidermal cell autophagy could be an attractive therapeutic strategy for diabetic wounds and that the process is mediated by the HIF-1α/TGF-β1/SMAD pathway.
Patient-Derived Intrafemoral Orthotopic Xenografts of Peripheral Blood or Bone Marrow from Acute Myeloid and Acute Lymphoblastic Leukemia Patients: Clinical Characterization, Methodology, and Validation
Authors: Bi Lintao , Li Jun , Chen Hongkui , Zhao ShiZhu , Wen Danyi 
Published: March 2022

Abstract
Acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) are malignant clonal diseases of the hematopoietic system with an unsatisfactory overall prognosis. The main obstacle is the increased resistance of AML and ALL cells to chemotherapy. The development and validation of new therapeutic strategies for acute leukemia require preclinical models that accurately recapitulate the genetic, pathological, and clinical features of acute leukemia. A patient-derived xenograft (PDX) model is established by transplanting patient tumor tissue or cells into immunocompromised or humanized mice. They closely resemble human tumor progression and microenvironment and are more reliable translational research tools than other models. A patient-derived orthotopic xenograft (PDOX) model that transplants tumor cells into the corresponding anatomical site in mice better recapitulates human tumor behavior than subcutaneous or intravenous xenografts. In this study, we established bone marrow and peripheral blood cell models of AML and ALL patients, characterized their pathology, cytology, and genetics, and compared the model's characteristics and drug responsiveness with those of the corresponding patients.
MiR-17-5p/RRM2 Regulated Gemcitabine Resistance in Lung Cancer A549 Cells
Authors: Ma Xuan , Fu Tian , Ke Zhi-Yin , Du Shen-Lin , Wang Xue-Chun , Zhou Ning , Liu Yong-Jun , Liang Ai-ling 
Published: March 2022

Abstract

Background
To investigate the roles of miR-17-5p/RRM2 in A549/G+.
Methods
Cell survival was analyzed by CCK8 kit and clone formation experiment; The mRNA expression level was detected by qRT-PCR, and the protein level was compared by Western blotting; And flow cytometry for cell cycle detection; The target gene of miR-17-5p was verified by double luciferase activity experiment.
Results
CCK8 assay displayed that miR-17-5p’s overexpression can let A549/G + cells turn into sensitive ones; in turn, in the case of inhibition of expression, miR-17-5p can make A549/G- cells turn into resistance ones. And similar results were obtained in cell clone formation experiment. Cell cycle analysis showed that miR-17-5p’s overexpression increased the number of G1 phase cells and reduced the number of S phase cells in A549/G + cells; Conversely, lowing expression level of miR-17-5p yielded the opposite results in A549/G- cells. Western blot results showed that when miR-17-5p was expressed highly, the expression levels of cell cycle related proteins, CCNE1, CCNA2 and P21 decreased in A549/G + cells; Conversely, inhibition of miR-17-5p expression yielded the opposite results too in A549/G- cells. Western blot analysis of signal pathway proteins and the results showed that PTEN and PI3K expressed higher, but p-PTEN expressed lower in A549/G + cells. After miR-17-5p overexpressed in A549/G + cells, the level of p-PTEN increased, and the level of p-AKT decreased; when miR-17-5p expressed low in A549/G-cells, the expression of p-PTEN and p-AKT were opposite. The dual-luciferase experiment demonstrated that RRM2 was the target gene of miR-17-5p. The restorative experiments of RRM2 verified it.
Conclusion
This article suggested that the miR-17- 5p/RRM2 axis could adjust gemcitabine-resistance in A549 cells and might associate with p-PTEN/PI3K/AKT signal pathway.
Evaluation of the residual efficacy and physical durability of five long-lasting insecticide- treated nets (LLINs) in Senegal
Authors: Diouf El Hadji , Diouf Mbaye , Dieme Constentin , Swamidoss Isabel , Ngom El hadji M. , Senghor Massila W. , Mbaye Modou , Konaté Abdoulaye , Coulibaly Youssouph , Dome Tine , Dia Ibrahima , Dotson Ellen M. , Faye Ousmane , Konaté Lassana 
Published: March 2022

Abstract
Background
The preventive and curative strategies of malaria are based on promoting the use of long-lasting insecticidal bed nets (LLINs) and treating confirmed cases with artemisinin-based combination therapies. These strategies have led to a sharp decline in the burden of malaria, which remains a significant public health problem in sub-Saharan countries. The objective of this study was to determine and compare the residual efficacy of LLINs recommended by the World Health Organization (WHO).
Methods
The study was conducted in six villages in two sites in Senegal located in the Sahelo-Sudanian area of the Thiès region, 70 km from Dakar and in Mbagame, a semi-urban zone in the Senegal River Valley. A census was conducted of all sleeping places in each household to be covered by LLINs. Five brands of LLIN were distributed, and every six months, retention rates, net use, maintenance, physical integrity, insecticide chemical content, and biological efficacy were examined for each type of LLIN.
Results
A total of 3012 LLINs were distributed in 1249 households in both sites, with an average coverage rate of 94% (95% CI 92.68–95.3). After 36 months, the average retention rate was 12.5% and this rate was respectively 20.5%, 15.1%, 10%, 7%, and 3% for Olyset Net®, Dawa Plus® 2.0, PermaNet® 2.0, NetProtect® and Life Net®, respectively. The proportion of LLINs with holes and the average number of holes per mosquito net increased significantly during each follow-up, with a large predominance of size 1 (small) holes for all types of LLINs distributed. During the three-year follow-up, bioassay mortality rates of a susceptible strain of insectary reared Anopheles coluzzii decreased in the following net types: in Dawa Plus® 2.0 (100–51.7%), PermaNet® 2.0 (96.6–83%), and Olyset Net® (96.6–33.3%). Mortality rates remained at 100% in Life Net® over the same time period. After 36 months, the average insecticide content per brand of LLIN decreased by 40.9% for Dawa Plus® 2.0, 31% for PermaNet® 2.0, 39.6% for NetProtect® and 51.9% for Olyset Net® and 40.1% for Life Net.
Conclusions
Although some net types retained sufficient insecticidal activity, based on all durability parameters measured, none of the net types survived past two years.
Effect of Aqueous Leaf Extract of Hamelia patens jacq. On Some Biochemical Parameters in Alloxan- Induced Diabetic Albino Rats
Authors: Caleb Nwaogwugwu Joel 
Published: March 2022

Abstract
Aim: Effect of aqueous leaf extract of Hamelia patens jacq. on some biochemical parameters in alloxan-induced diabetic albino rats was determined.Method: The experimental animals (male Albino rats) were divided randomly into six groups of eight animals each. The test groups (2-6) were starved overnight followed by intraperitoneal administration of alloxan at 160mg/kg body weight. Treatment with the plant extract was for fourteen days. Group 1 was the normal control, group 2; diabetic untreated animals, group 3 received the standard drug gliclazide at 58mg/ml, groups 4, 5 and 6 were the extract treated groups at 400mg/kg body weight, 800mg/kg body weight and 1200mg/kg body weight respectively.Results/discussion: The mean lethal dose of the plant extract was above 5000mg/kg body weight. Total protein increased significantly (P<0.05) in the extract treated groups while, aspartate amino transferase (AST), alanin transaminase (ALT), alkaline phosphatase (ALP) had significant decreases (P<0.05) when compared to group 2. Malondialdehyde (MDA), decreased significantly (P<0.05) in the group that received the standard drug alongside the extract treated groups when compared to group 2, while Glutathione (GSH) and Superoxide dismutase had a significant decrease in groups 2 and 3 at (P<0.05) and was non significant in the extract treated groups at (P>0.05). The C-peptides increased significantly (P<0.05) in groups 4 to 6 when compared to the group 2 that had a higher significant increase (P<0.05).Conclusion: The results show that aqueous leaf extract of Hamelia patens may possess anti-diabetic, anti-inflammatory, antihepatotoxic, antianemic, antioxidant and antilipidemic properties at the tested doses.
Evaluation of Enlarged Laminectomy with Lateral Mass Screw Fixation in Relieving Nerve Root Symptoms and Correcting Kyphosis for Cervical Myelopathy and Radiculopathy
Authors: Fang Zhao , Xu Tiantong , Li Yuqiao , Luo Gan , Yang Houzhi , Wang Ziqi 
Published: March 2022

Abstract
Purpose
This study aimed to compare the surgical efficacy of enlarged laminectomy with lateral mass screw fixation (EL-LMSF) and anterior cervical corpectomy decompression and fusion (ACCF) for multilevel cervical myelopathy and radiculopathy (CMR) related to kyphosis.
Methods
We retrospectively analyzed 75 patients treated with either EL-LMSF or ACCF surgery for multilevel CMR in the study. Operative time, blood loss, the incidence of surgical complications, and the magnitude of the regional sagittal Cobb angle were compared. According to the Japanese Orthopedic Association (JOA) Scores, Visual Analog Scale (VAS) scores for the neck, radiological parameters and clinical function scores pre- and postoperatively were compared in each patient to analyze the surgical efficacy of EL-LMSF and ACCF.
Results
The JOA score, VAS score for neck pain and C2-C7 angle before the operation were not significantly different between the ACCF group and the EL-LMSF group. The ACCF group was performed over a mean of 2.39 levels (range, 2–3 levels) and all patients were followed up for more than 1 year after surgery. Analysis of the follow-up data showed a shorter operation time (103 ± 22 min vs. 125 ± 37 min, P = 0.000) and less blood loss (78 ± 15 ml vs. 226 ± 31 ml, P = 0.000) in the ACCF group than the EL-LMSF group. Patients treated with EL-LMSF indicated lower VAS scores for the neck (1.3 ± 1.7 vs. 3.3 ± 1.3, P = 0.003) and better cervical lordosis (10.7 ± 4.2° vs. 8.5 ± 3.5°, P = 0.013) than the ACCF group.No difference in the JOA score (14.1 ± 1.7 vs. 13.5 ± 2.1, P = 0.222) was noted between the two groups. During the follow-up period, 15.2% of patients in the ACCF group had complications, including 2 cases with transient dysphagia, 1 case with C5 palsy, 1 case with axial symptoms, and 1 with screw pullout. In the EL-LMSF group, only 9.5% of cases experienced complications, including 3 cases of axial pain and 1 case of epidural hematoma.
Conclusions
EL-LMSF for the treatment of multilevel CMR is an effective and safe strategy for relieving nerve root symptoms and restoring cervical curvature. However, high-quality, large-scale clinical trials with long-term follow-up are urgently warranted.
A radiomics-based machine learning pipeline to distinguish between metastatic and healthy bone using lesion-center-based geometric regions of interest
Authors: Naseri Hossein , Skamene Sonia , Tolba Marwan , Faye Mame Daro , Ramia Paul , Khriguian Julia , Patrick Haley , Andrade Aixa X , David Marc , Kildea John 
Published: March 2022

Abstract
Radiomics-based machine learning classifiers have shown potential for detecting bone metastases (BM) and for evaluating BM response to radiotherapy (RT). However, current radiomics pipelines require large datasets of images with expert-segmented 3D regions of interest (ROIs). Full ROI segmentation is time consuming and oncologists often outline just RT treatment fields in clinical practice. This presents a challenge for real-world radiomics research. As such, a method that simplifies BM identification but does not compromise the power of radiomics is needed. The objective of this study was to investigate the feasibility of a radiomics pipeline for BM detection using lesion-center-based geometric ROIs. The simulation-CT images of 170 patients with non-metastatic lung cancer and 189 patients with spinal BM were used. The point locations of 631 BM and 674 healthy bone (HB) regions were identified by experts. ROIs with various geometric shapes were centered and automatically delineated on the identified locations, and 107 radiomics features were extracted. Various re-sampling techniques, feature selection methods, and machine learning classifiers were evaluated. Our point-based radiomics pipeline was successful in differentiating BM from HB. This approach greatly simplifies the process of preparing images for use in radiomics studies and avoids the bottleneck of full ROI segmentation.
Should Anti-thymocyte Globulin be Added in Post-transplant Cyclophosphamide Based Matched Unrelated Donor Peripheral Blood Stem Cell Transplantation for Acute Myeloid Leukemia? A Study on Behalf of the Acute Leukemia Working Party of the EBMT
Authors: Spyridonidis Alexandros , Labopin Myriam , Brissot Eolia , Moiseev Ivan , Cornelissen Jan , Choi Goda , Ciceri Fabio , Vydra Jan , Reményi Péter , Rovira Montserrat , Meijer Ellen , Labussière-Wallet Hélène , Blaise Didier , Gorkom Gwendolyn , Kröger Nicolaus , Koc Yener , Giebel Sebastian , Bazarbachi Ali , Savani Bipin , Nagler Arnon , Mohty Mohamad 
Published: March 2022

Abstract
Background: Post-transplant cyclophosphamide (PTCY) is increasingly used for allogeneic peripheral blood stem cell transplantation (allo-PBSCT) from an HLA-matched unrelated donor (MUD) as an alternative to the standard anti-thymocyte globulin (ATG) graft-versus-host disease (GvHD) prophylaxis. Following the demonstration that the use of PBSC haploidentical grafts results in more GvHD than bone marrow grafts, groups have attempted to reduce GvHD in haploidentical-PBSCT by adding ATG to PTCY. The experience of combined PTCY+ATG in the MUD allo-PBSCT is minimal and whether ATG brings any added value when PTCY is used in this setting is still unclear. Methods: In this registry-based study, we compared outcomes of 421 patients with PTCY and 151 patients with PTCY+ATG who underwent a first MUD allo-PBSCT for acute myeloid leukemia (AML) in complete remission. Results: Characteristics of PTCY and PTCY+ATG patients were well balanced, including the number of additional immunosuppressive drugs, with the only significant difference between the two cohorts being the median year of transplant, and the follow-up period (19.6 versus 31.1 months, respectively, p<0.0001). Engraftment failure was low in both groups. Overall, two-year survival was 69.9% vs 67.1% in PTCY and PTCY+ATG, respectively, with deaths related to relapse (39% vs 43.5%), infection (21.9% vs 23.9%) or GvHD (17.1% vs 17.4%) not differing between groups. On univariate comparison, a significantly lower rate of extensive chronic GvHD was found when ATG was added (9.9% vs 21%, p=0.029), a finding which was not confirmed in the multivariate analysis. The Cox proportional-hazards model showed no difference between PTCY+ATG and PTCY alone with respect to acute and chronic GvHD of all grades. In univariate and multivariate analyses, no statistical differences were observed for non-relapse mortality, relapse, leukemia-free survival, overall survival, and GvHD-free, relapse-free survival between study cohorts. Conclusions: To date, the question of the best combination of GvHD-preventing drugs in the MUD-PBSCT setting remains unanswered. Our results highlight that in PTCY-based MUD-PBSCT for AML, the addition of ATG does not provide any extra benefit in terms of further GvHD reduction, better GRFS or better survival.