bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hornerin deposits in neuronal intranuclear inclusion disease: direct identification of proteins with compositionally biased regions in inclusions
Authors: Park Hongsun , Yamanaka Tomoyuki , Toyama Yumiko , Fujita Atsushi , Doi Hiroshi , Nirasawa Takashi , Murayama Shigeo , Matsumoto Naomichi , Shimogori Tomomi , Ikegawa Masaya , Haltia Matti J. , Nukina Nobuyuki 
Published: March 2022

AbstractNeuronal intranuclear inclusion disease (NIID) is a neurodegenerative disorder, characterized by the presence of eosinophilic inclusions (NIIs) within nuclei of central and peripheral nervous system cells. This study aims to identify the components of NIIs, which have been difficult to analyze directly due to their insolubility. In order to establish a method to directly identify the components of NIIs, we first analyzed the huntingtin inclusion-rich fraction obtained from the brains of Huntington disease model mice. Although the sequence with expanded polyglutamine could not be identified by liquid-chromatography mass spectrometry, amino acid analysis revealed that glutamine of the huntingtin inclusion-rich fraction increased significantly. This is compatible with the calculated amino acid content of the transgene product. Therefore, we applied this method to analyze the NIIs of diseased human brains, which may have proteins with compositionally biased regions, and identified a serine-rich protein called hornerin. Since the analyzed NII-rich fraction was also serine-rich, we suggested hornerin as a major component of the NIIs. A specific distribution of hornerin in NIID was also investigated by Matrix-assisted laser desorption/ionization imaging mass spectrometry and immunofluorescence. Finally, we confirmed a variant of hornerin by whole-exome sequencing and DNA sequencing. This study suggests that hornerin may be related to the pathological process of this NIID, and the direct analysis of NIIs, especially by amino acid analysis using the NII-rich fractions, would contribute to a deeper understanding of the disease pathogenesis.
Preliminary fluid geochemical survey in Tete Province and prospective development of geothermics in Mozambique
Authors: Procesi M. , Marini L. , Cinti D. , Sciarra A. , Basile P. , Mazzoni T. , Zarlenga F. 
Published: March 2022

AbstractAn evaluation of the feasible development of geothermal energy in Mozambique is proposed based on some thermal springs geochemical characterization in the Tete region. Chemical and isotopic data suggest that the springs have a meteoric origin and do not show connection with any active magmatic system. The proposed circulation model suggests high depths infiltration of meteoric waters along faults and fractures in a system characterised by discrete permeability and reservoir temperature between 90 and 120 °C. These results, jointly with low salinity fluids and corrosive components absence suggest that the geothermal system may be conveniently exploited for direct and indirect uses.
Enhancement of equatorial OI(1D) emissions at midnight
Authors: Chiang Chih-Yu , Chang Tzu-Fang , Tam Sunny W. Y. 
Published: March 2022

AbstractA number of recent studies have highlighted observational evidence of midnight brightness of the 630.0 nm nightglow, which is usually related to the midnight temperature maximum (MTM) effect. In this report, MTM-related enhancements of the 630.0 nm airglow around midnight are observed through images from the ISUAL/FORMOSAT-2 satellite. The data statistics are classified into three specific types (no-peak, single-peaked, and double-peaked events) and separated into the different seasons. In order to understand the influences of geomagnetic conditions, the statistical analyses are also separated into two regions. One is collected from the region whose geomagnetic equator is north of the geographic equator, and the other is collected from the region whose geomagnetic equator is south of the geographic equator. The results show that the single-peaked brightness often appears between the geographic equator and the geomagnetic equator. The double-peaked brightness appears simultaneously on the two sides of the region sandwiched by the two equators. Coupled with the summer-to-winter neutral wind generated by seasonal effects, one side of brightness could be enhanced or disappear due to the plasma moving along the field line. The no-peak events mainly occur close to May–July, which may be due to the effect of ionospheric annual anomalies. Overall, the statistical results for the occurrence rate show strong seasonal variations with different cycles at different longitudinal regions for all three types of events.
Graphical Abstract
Potential role of dental pulp stem cells conditioned medium for odontoblastic differentiation
Authors: Koh Benson , Ab Rahman Farynna Hana , Matlan Najwa Amira , Rajan Manissha , Musta’ain Aimi Yasmin , Mohd Jeffry Lee Mohamad Ridhwan , Ramli Roszalina , Mohd Yunus Siti Salmiah , Binti Hj Idrus Ruszymah , Yazid Muhammad Dain 
Published: March 2022

Abstract
Background
Functional bioengineered tooth regeneration using autologous or allogeneic alternative differentiated cells sources are thought to have a great potential in replacing conventional dentures. This study investigated the potential of dental pulp stem cells (DPSCs) conditioned medium for odontoblastic differentiation of Wharton’s jelly mesenchymal stem cells (WJMSCs). The DPSCs derived from healthy adult permanent first molars were cultured at high confluence prior to conditioned medium collection. The WJMSCs were cultured in six different treatments, with varying ratios of culture media to DPSCs-conditioned medium. MTT assay was used to measure the rate of proliferation of WJMSCs, while immunocytochemistry staining was utilised to detect the expression of dental matrix protein 1 (DMP-1). The deposited calcium was detected and analysed via Alizarin-Red Staining (ARS).

Results
It was found that the proliferation of WJMSCs cultured under the mixture of complete medium and DPSCs conditioned medium showed significantly lower than the control; presumably the cells started to exit proliferative state prior differentiation. In 14 days of induction, the cells in all treatments showed osteoblastic-like morphology, calcium compound deposits were observed at day 7, 10 and 14 of differentiation suggested that DPSCs conditioned medium could lead to osteoblastic/odontoblastic differentiation. However, the DMP-1 protein can be seen only expressed minimally at day 14 of conditioned medium induction.

Conclusions
In conclusion, DPSCs conditioned medium appeared as a potential odontoblastic induction approach for WJMSCs. To further investigate the stimulatory effects by DPSCs conditioned medium, specific signalling pathway need to be elucidated to enhance the differentiation efficiency.
A collagen membrane for periosteal expansion osteogenesis using a timed-release system in rabbit calvaria
Authors: Yamauchi Kensuke , Imoto Kazuhiro , Odajima Kenji , Morishima Hiromitsu , Shimizu Yoshinaka , Nogami Shinnosuke , Takahashi Tetsu 
Published: March 2022

Abstract
Background
The purpose of this study was to evaluate the effects of resorbable membranes, combined with a shape memory alloy (SMA) mesh device, on bone formation using a timed-release system for periosteal expansion osteogenesis (TIME-PEO).

Materials and methods
Twelve Japanese white rabbits were used in this study. An SMA device was inserted under the forehead periosteum, pushed and bent for attachment to the bone surface, and then fixed using resorbable thread. The rabbits were divided into four groups: C1 (5 weeks postoperatively without membrane), C2 (8 weeks postoperatively without membrane), E1 (5 weeks postoperatively with membrane), and E2 (8 weeks postoperatively with membrane). The rabbits were killed 5 or 8 weeks after the operation and the newly formed bone was assessed histologically and radiographically.

Results
SMA devices, concealed under soft tissue until the time of euthanasia, did not cause active inflammation. The mean activation height, from the original bone surface to the midpoint of the mesh, was 3.1 ± 0.6 mm. Newly formed bone was observed, and most of the subperiosteal space underneath the device was occupied by fibrous tissue. Immature bone was present at the outer surface of the original skull bone in all groups. On histomorphometric analysis, there was no significant difference in the volume of the new bone between C1 and E1 (p = 0.885), and C2 and E2 (p = 0.545).

Conclusions
PEO using an SMA mesh device, which is based on guided bone regeneration (in atrophic alveolar bone), shows promise as an alternative for bone augmentation, irrespective of whether a resorbable membrane is used.
Strategies and tactics to perform safe pancreaticoduodenectomy for 94-year-old patient: report of a case
Authors: Suyama Yu , Haruki Koichiro , Hamura Ryoga , Tsunematsu Masashi , Shirai Yoshihiro , Taniai Tomohiko , Yanagaki Mitsuru , Furukawa Kenei , Onda Shinji , Shiba Hiroaki , Ikegami Toru 
Published: March 2022

Abstract
Background
Despite improvement of postoperative management, pancreatoduodenectomy still has a high rate of major complications. Therefore, careful assessment is critically important when we consider high risk surgery for extremely elderly patients.

Case presentation
A 94-year-old man, who suffered dark urine, epigastric pain, and loss of appetite, was diagnosed as bile duct cancer and underwent endoscopic retrograde biliary drainage. He has past history of hypertension and paroxysmal atrial fibrillation. Computed tomography (CT) showed a nodule in the lower bile duct, which was slowly enhanced by dynamic CT. The patient was evaluated whether he overcomes pancreatoduodenectomy by cardiac ultrasonography, brain magnetic resonance angiography, nutritional evaluation (rapid turnover proteins), and CT-based general assessment, including sarcopenia and osteopenia. The patient was independent in activities of daily living and has enough ejection fraction of 65%, and examinations revealed no impairment of cognitive function, sarcopenia, and osteopenia. With a diagnosis of bile duct cancer with no distant metastasis, the patient underwent subtotal stomach-preserving pancreatoduodenectomy with lymph node dissection. Operation time was 299 min and estimated blood loss was 100 ml. Pathological examination revealed papillary adenocarcinoma of the bile duct (pT3N1M0 Stage IIIB). Enteral nutrition was given through jejunostomy and then the patient started oral intake after an evaluation of swallowing function. Postoperative course was uneventful and all drains including pancreatic duct stent, biliary stent, and jejunostomy were removed by 3 weeks after operation. The levels of rapid turnover proteins dropped at postoperative day 7, but recovered at 1 month after operation via appropriate nutrition and rehabilitation. He remains well with no evidence of tumor recurrence as of 1 year after resection.

Conclusions
We herein report successfully treated cases of bile duct cancer in 94-year-old patient by pancreatoduodenectomy with careful evaluation of osteopenia, sarcopenia and nutrition.
Exercise Training and Natural Killer Cells in Cancer Survivors: Current Evidence and Research Gaps Based on a Systematic Review and Meta-analysis
Authors: Valenzuela Pedro L. , Saco-Ledo Gonzalo , Santos-Lozano Alejandro , Morales Javier S. , Castillo-García Adrián , Simpson Richard J. , Lucia Alejandro , Fiuza-Luces Carmen 
Published: March 2022

Abstract
Background
Exercise training can positively impact the immune system and particularly natural killer (NK) cells, at least in healthy people. This effect would be of relevance in the context of cancer given the prominent role of these cells in antitumor immunity. In this systematic review and meta-analysis, we aimed to summarize current evidence on the effects of exercise training on the levels and function of NK cells in cancer survivors (i.e., from the time of diagnosis until the end of life).

Methods
Relevant articles were searched in PubMed, Scopus, Web of Science and Cochrane Central Register of Controlled Trials (until January 11, 2022). Randomized controlled trials (RCT) of exercise training (i.e., non-acute) interventions vs usual care conducted in cancer survivors and assessing NK number and/or cytotoxic activity (NKCA) before and upon completion of the intervention were included. Methodological quality of the studies was assessed with the PEDro scale, and results were meta-analyzed using a random effects (Dersimoian and Laird) model.

Results
Thirteen RCT including 459 participants (mean age ranging 11–63 years) met the inclusion criteria. Methodological quality of the studies was overall fair (median PEDro score = 5 out of 10). There was heterogeneity across studies regarding cancer types (breast cancer, non-small cell lung cancer and other solid tumors), treatment (e.g., receiving vs having received chemotherapy), exercise modes (aerobic or resistance exercise, Tai Chi, Yoga) and duration (2–24 weeks). No consistent effects were observed for NK number in blood (mean difference [MD]: 1.47, 95% confidence interval [CI] − 0.35 to 3.29, p = 0.113) or NKCA as assessed in vitro (MD: − 0.02, 95%CI − 0.17 to 0.14, p = 0.834). However, mixed results existed across studies, and some could not be meta-analyzed due to lack of information or methodological heterogeneity.

Conclusions
Current evidence does not support a significant effect of exercise training intervention on NK cells in blood or on their ‘static response’ (as assessed in vitro) in cancer survivors. Several methodological issues and research gaps are highlighted in this review, which should be considered in future studies to draw definite conclusions on this topic.
Equipping Physical Activity Leaders to Facilitate Behaviour Change: An Overview, Call to Action, and Roadmap for Future Research
Authors: Stevens Mark , Rees Tim , Cruwys Tegan , Olive Lisa 
Published: March 2022

AbstractAddressing high and stagnant physical inactivity rates remains a pervasive challenge for researchers, and a priority for health organisations, governments, and physical activity practitioners. Leaders are a prevailing feature of numerous physical activity contexts and can fundamentally influence people’s physical activity behaviours and experiences. In line with this, fitness companies and organisations commonly claim that the leaders of their classes, groups, or sessions will motivate, inspire, and ensure exercisers achieve their goals. We argue, however, that there is insufficient evidence regarding how leaders can best facilitate positive behaviours among, and outcomes for, group members to be confident that these claims are translating into strong physical activity leadership on the ground. In this article, we therefore call for research that equips leaders with greater knowledge and practical guidelines for how to maximise their effectiveness. To facilitate such research, we provide an overview of research that has examined the most effective ways for physical activity leaders to promote health-enhancing behaviours (e.g. greater participation) and positive experiences that may lead to such behaviours (e.g. greater exercise enjoyment) among those they lead. Then, with the shortcomings of this extant research in mind, we outline four broad recommendations for future research: (a) conduct research in novel and varied contexts, (b) focus on insufficiently active populations, (c) utilise qualitative methods, and (d) focus on translation and implementation. Such research would, we believe, generate knowledge that enables physical activity leaders to capitalise on their potential to be powerful agents of behaviour change.
The Sickle Cell Disease Functional Assessment (SCD-FA) tool: a feasibility pilot study
Authors: Oyedeji Charity I. , Hall Katherine , Luciano Alison , Morey Miriam C. , Strouse John J. 
Published: March 2022

Abstract
Background
The life expectancy for individuals with sickle cell disease (SCD) has greatly increased over the last 50 years. Adults with SCD experience multiple complications such as cardiopulmonary disease, strokes, and avascular necrosis that lead to limitations that geriatric populations often experience. There are no dedicated instruments to measure functional decline and functional age to determine risk of future adverse outcomes in older adults with SCD. The objective of this study was to assess the feasibility of performing the Sickle Cell Disease Functional Assessment (SCD-FA).

Methods
We enrolled 40 adults with SCD (20 younger adults aged 18–49 years as a comparison group and 20 older adults aged 50 years and older) in a single-center prospective cohort study. Participants were recruited from a comprehensive sickle cell clinic in an academic center in the southeastern United States. We included measures validated in an oncology geriatric assessment enriched with additional physical performance measures: usual gait speed, seated grip strength, Timed Up and Go, six-minute walk test, and 30-second chair stand. We also included an additional cognitive measure, which was the Montreal Cognitive Assessment, and additional patient-reported measures at the intersection of sickle cell disease and geriatrics. The primary outcome was the proportion completing the assessment. Secondary outcomes were the proportion consenting, duration of the assessment, acceptability, and adverse events.

Results
Eighty percent (44/55) of individuals approached consented, 91% (40/44) completed the SCD-FA in its entirety, and the median duration was 89 min (IQR 80–98). There were no identified adverse events. On the acceptability survey, 95% (38/40) reported the length as appropriate, 2.5% (1/40) reported a question as upsetting, and 5% (2/40) reported portions as difficult. Exploratory analyses of physical function showed 63% (25/40) had a slow usual gait speed (< 1.2 m/s).

Conclusion
The SCD-FA is feasible, acceptable, and safe and physical performance tests identified functional impairments in adults with SCD. These findings will inform the next phase of the study where we will assess the validity of the SCD-FA to predict patient-important outcomes in a larger sample of adults with SCD.
Modification of implant surfaces to stimulate mesenchymal cell activation
Authors: Robo Ilma , Heta Saimir , Papakozma Dhimitri , Ostreni Vera 
Published: March 2022

Abstract
Background
The process of osteointegration, as key point has the activation of mesenchymal cells at implant-bone interspace, their differentiation into osteoblasts and connection between the implant surface and the surrounding bone.

Main text
Implant surfaces composed by biocompatible, organism-friendly materials require changes in content and surface morphology; changes that may further stimulate mesenchymal cell activation.
The way the implant surfaces are affected with advantages and disadvantages, that typically bring each methodology, is also the purpose of this study. The study is of review type, based on finding articles about implant surface modification, with the aim of promoting the mesenchymal cell activation, utilizing keyword combination.

Conclusions
Implant success beyond the human element of the practicioner and the protocol element of implant treatment, also relies on the application of the right type of implant, at the right implant site, in accordance with oral and individual health status of the patient. Implant success does not depend on type of "coating" material of the implants. Based at this physiological process, the success or implant failure is not a process depending on the type of selected implant, because types of synthetic or natural materials that promote osteointegration are relatively in large number.
Surgical site infection after wound closure with staples versus sutures in elective knee and hip arthroplasty: a systematic review and meta-analysis
Authors: van de Kuit A. , Krishnan R. J. , Mallee W. H. , Goedhart L. M. , Lambert B. , Doornberg J. N. , Vervest T. M. J. S. , Martin J. 
Published: March 2022

AbstractPurposeThis systematic review and meta-analysis aimed to study surgical site infection of wound closure using staples versus sutures in elective knee and hip arthroplasties.MethodsA systematic literature review was performed to search for randomized controlled trials that compared surgical site infection after wound closure using staplesversussutures in elective knee and hip arthroplasties. The primary outcome was surgical site infection. The risk of bias was assessed with the Cochrane risk of bias assessment tool. The relative risk and 95% confidence interval with a random-effects model were assessed.ResultsEight studies were included in this study, including 2 studies with a low risk of bias, 4 studies having ‘some concerns’, and 2 studies with high risk of bias. Significant difference was not found in the risk of SSI for patients with staples(n = 557)versussutures(n = 573) (RR: 1.70, 95% CI: 0.94–3.08, I2 = 16%). The results were similar after excluding the studies with a high risk of bias (RR: 1.67, 95% CI: 0.91–3.07, I2 = 32%). Analysis of studies with low risk of bias revealed a significantly higher risk of surgical site infection in patients with staples (n = 331) compared to sutures (n = 331) (RR: 2.56, 95% CI: 1.20–5.44, I2 = 0%). There was no difference between continuous and interrupted sutures (P > 0.05). In hip arthroplasty, stapling carried a significantly higher risk of surgical site infection than suturing (RR: 2.51, 95% CI: 1.15–5.50, I2 = 0%), but there was no significant difference in knee arthroplasty (RR: 0.87, 95% CI: 0.33–2.25, I2 = 22%;P > 0.05).ConclusionsStapling might carry a higher risk of surgical site infection than suturing in elective knee and hip arthroplasties, especially in hip arthroplasty.
Prognosis of COVID-19 in respiratory allergy: a systematic review and meta-analysis
Authors: El Shahawy Alia Abdelmonem , Oladimeji Kelechi Elizabeth , Hamdallah Aboalmagd , Saidani Amal , Abd-Rabu Rami , Dahman Nesrine Ben Hadj 
Published: March 2022

Abstract
Background
Do underlying allergic respiratory diseases such as asthma and rhinitis predispose to a severe coronavirus (COVID-19) infection? We conducted this systematic review to map out and synthesize evidence of published literature.

Main body of the abstract
We searched five bibliographic databases for articles published between 1 January and 15 November 2020 using keywords: “COVID” AND “Allergic disease,” “Prognosis and COVID-19,” “SARS-CoV-2,” “Asthma,” “Allergic rhinitis.” We synthesized 32 eligible articles from a total of 11,376 articles retrieved from the search process. The profile of allergic respiratory conditions was identified, and only seven studies reported on the treatment administered. No significant difference was observed concerning the prevalence of COVID-19 in individuals with allergic asthma and those with non-allergic asthma (RR = 0.61, p = 0.08). The mortality rate significantly decreased in COVID-19-infected patients with asthma than patients without asthma (RR = 0.63, p = 0.04).

Short conclusion
There is little evidence available on the role of asthma medications and risk factors influencing the prognostic outcomes for COVID-19 individuals with respiratory allergies, which invites further research.