bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating population-level effects into the regulatory assessment of endocrine disrupting substances
Authors: Hazlerigg Charles R E , Tagliati Alice , Forbes Valery E , Gergs Andre , Hallmark Nina , Maltby Lorraine , Weltje Lennart , Wheeler James R 
Published: January 2025

Abstract
Population modeling, field studies, and monitoring approaches have all been proposed for assessing the relevance of adverse effects of endocrine disrupting chemicals (EDCs) at the population level for nontarget (wild) vertebrates, but how these approaches should be used in the regulatory hazard assessment is unclear and not detailed in the relevant European Guidance Document. A literature review focused on identifying published approaches assessing the population relevance of adverse effects from EDCs was performed, and, subsequently, 47 primary research papers were evaluated. By extracting from these sources, a novel approach was developed with guiding principles for assessing adverse effects of EDCs at the population level considering (i) choice of focal species, scenarios (and models), (ii) the individual level apical endpoints to be considered, (iii) the magnitude of effect to be imposed, (iv) for what duration effects should be imposed, (v) whether individuals repairing the damage from exposure should be included, (vi) the population-level endpoints to be considered, and (vii) what threshold to set for defining an adverse effect at this level. Recommendations for modeling and field and monitoring studies are included. Case studies are also presented to demonstrate how the proposed approach might be implemented. Although some aspects (e.g., choice of focal species, model/experimental scenario, monitoring study assessment) require further consideration, this should not prevent the use of this approach in a regulatory EDC assessment context. As such, we propose that the approach be used immediately to implement population modeling and perform field studies within this regulatory context. We envisage that consistent application of these principles will encourage regulatory developments in this critical area to provide a much needed level of clarity in the EDC assessment for all stakeholders.
Global advances and innovations in bacteria-based biosorption for heavy metal remediation: a bibliometric and analytical perspective
Authors: Syarifuddin Syarifuddin , Suryani Sri , Tahir Dahlang 
Published: January 2025

Abstract
Industrialization and urbanization have significantly escalated the discharge of heavy metals into aquatic environments, posing serious ecological and public health risks. This study explores the global research landscape of bacterial biosorption for heavy metal removal, emphasizing advancements in methodologies and technologies that have redefined this field. A bibliometric analysis of 298 publications (1987–2024) was conducted to identify key trends, collaboration networks, and innovations. Notable advancements include the integration of nanotechnology, which has enhanced adsorption efficiency and selectivity for specific metals, and genetic engineering approaches that optimize bacterial strains for higher adsorption capacity. Furthermore, these developments have transformed traditional remediation strategies by providing cost-effective, sustainable, and scalable solutions for industries such as textiles, mining, and energy production. This study underscores the practical relevance of bacterial biosorption in wastewater treatment, achieving removal efficiencies exceeding 99% in some cases, as demonstrated by Aspergillus versicolor and Shewanella oneidensis MR-1. By bridging scientific innovation with environmental sustainability, this research highlights bacterial biosorption as a pivotal green technology, offering actionable insights for industrial applications and global sustainability goals.
Aging oocytes: exploring apoptosis and its impact on embryonic development in common carp (Cyprinus carpio)
Authors: Konar Essaikiammal Sodalai Muthu , Brachs Sebastian , Mai Knut , Waghmare Swapnil Gorakh , Policar Tomas , Samarin Azadeh Mohagheghi , Samarin Azin Mohagheghi 
Published: January 2025

Abstract
Ovulation, fertilization, and embryo development are orchestrated and synchronized processes essential for the optimal health of offspring. Postovulatory aging disrupts this synchronization and impairs oocyte quality. In addition, oocyte aging causes fertilization loss and poor embryo development. This investigation aimed to unravel the endpoint of in vitro oocyte aging in common carp (Cyprinus carpio) to understand the involvement of apoptosis in postovulatory oocyte death. It was observed that the fertilization ability significantly declined (P &lt; 0.001) at 8-h poststripping (HPS), subsequently triggering apoptosis in the advanced stage of oocyte aging, i.e., 48 HPS. This process included an increase in proapoptotic transcripts (fas, bax, cathepsin D, caspase 8, caspase 9, and caspase 3a) (P &lt; 0.05), elevated levels of caspase 3 protein (P &lt; 0.05), and activation of caspase 3 enzyme (P &lt; 0.001), a key player in apoptosis, in aging oocytes. Furthermore, the effects of oocyte aging on the embryonic apoptosis machinery were examined in embryos at 5-h postfertilization (HPF) and 24 HPF derived from fresh and aged oocytes. Expression of apoptotic genes and caspase enzyme activity remained at the basal level in 5 HPF (early blastula embryos) from both fresh and aged oocytes. In contrast, the zymogenic and active forms of caspase 3 increased in 24 HPF embryos from 8-h-aged oocytes (P &lt; 0.01) compared with those from fresh oocytes. Thus, apoptosis intensified in 24 HPF embryos from aged oocytes without affecting the apoptotic machinery of early blastula embryos. Our findings demonstrate that apoptosis initiated by the Fas/FasL system is an important physiological process accompanying oocyte aging in common carp.
Production of non-triple-helical collagen polypeptides under hypoxia and the implication for tumour
Authors: Sekine Kosuke , Tokunaka Kazuhiro , Tomura Arihiro , Sugihara Hidemitsu , Saijo Yuki , Shin Yongchol , Hayashi Toshihiko , Morita Makoto , Imamura Yasutada 
Published: January 2025

Abstract
Non-triple-helical collagen polypeptides (NTHs) are alternative gene products lacking the typical collagen triple-helical structure. This study investigated NTH production in tumour cells and tissues. NTH α1(IV) was detected in various human tumour cell lines and extracted from human lung cancer tissues and tumours in mice. NTH production was significantly affected by serum concentration and occurred under hypoxic or hypoxia-mimetic conditions, even with sufficient ascorbic acid. This suggests NTHs are produced under physiological hypoxia, potentially contributing to tumour angiogenesis. NTH production generally coincided with hypoxia-inducible factor-1α (HIF-1α) accumulation, except with cobalt chloride, indicating HIF-1α is not directly involved in NTH α1(IV) production. NTH electrophoretic mobility on SDS-PAGE was higher under hypoxia or deferoxamine treatment, likely due to suppressed lysyl hydroxylase 3 activity. This study demonstrates NTH production in tumour cells and tissues under hypoxia, suggesting their association with tumour angiogenesis and potential as therapeutic targets.
Prevalence of Alcohol Exposure in Burns-related Injuries
Authors: Orda Kathrin , Lau Georgina , Tracy Lincoln M , Mitra Biswadev 
Published: January 2025

Abstract
The aim of this registry-based cohort study was to quantify the prevalence, injury characteristics, and outcomes of alcohol exposure in burn-related injuries. All patients ≥ 18 years presenting to The Alfred Emergency & Trauma Centre, a major trauma center in Victoria, Australia, between January 1, 2019 and December 31, 2022 and recorded in the Victorian Adult Burns Service registry were included. An explicit chart review was performed to verify retrieved data, with alcohol exposure coded when documented in medical records, laboratory evidence of a blood alcohol concentration &gt; 2 mmol/L, or recorded in discharge ICD-10-AM codes. Among 1587 eligible patients, 251 (15.8%) had been exposed to alcohol prior to injury. Patients with alcohol exposure at the time of burn-related injury had higher rates of admission to the intensive care unit (28% vs 16.4%, P &lt; .001) and longer median hospital admission (10.1 vs 7.7 days, P &lt; .001). The significant impact of alcohol exposure on the overall health burden of burn injuries highlights the need for standardized and robust alcohol testing protocols to support targeted strategies to reduce the burden of alcohol-related burn injuries.
The human rights obligations of belligerent occupiers: Israel and the Gazan population
Authors: Bantekas Ilias , Jaber Safaa S 
Published: January 2025

Abstract
Following the Israeli disengagement from the Gaza Strip in 2005, the Israeli government declared that it was no longer an occupant. Even so, it redeployed its forces throughout the Strip’s external border, with entry and exit therefrom being wholly controlled by Israel’s Defense Forces. In 2024, the International Court of Justice issued an Advisory Opinion whereby it declared, among others, that Gaza, the West Bank, and East Jerusalem have been under continuous belligerent occupation that was moreover illegal. The Advisory Opinion thus confirmed that, despite Israeli statements to the contrary, it continues to be the occupying power of Gaza due to its effective control, notwithstanding physical military presence. As an occupant asserting effective control over Gaza, in addition to having an obligation to provide humanitarian guarantees under international humanitarian law to the civilian population in Gaza, Israel has a duty to provide fundamental human rights (HR) under international human rights law. Even if Israel were not considered an occupant of the territory, the amount of Israeli control over the Strip would certainly trigger the extraterritorial application of HR treaties and the full gamut of cascading obligations inherent therein.
Weight-bearing CT as an approach to assess femoral–acetabular displacement during external rotation stress in the hip
Authors: Rivas Dominic J. L , Gassmann Joshua M , Goetz Jessica E , Aitken Holly D , Davison John C , Miller Aspen , Willey Michael C 
Published: January 2025

Abstract
Hip dysplasia causes pathologic joint mechanics and can produce hip instability, leading to progressive joint degeneration and osteoarthritis. Weight-bearing computed tomography (WBCT) is an emerging technology that may enable quantification of femoral–acetabular displacement as an objective indicator of instability. To evaluate this potential, 10 patients indicated for periacetabular osteotomy to treat hip dysplasia and 10 healthy controls underwent two WBCT protocols. Participants were scanned in a neutral stance [weight-bearing (WB)] and again with the hip stressed in maximal external rotation (WB-stress), a position hypothesized to reproduce anterior instability. Clinical, nonweight-bearing computed tomography (CT) scans were available for patients with hip dysplasia. Congruency of the femoroacetabular joint space and position of the femoral head in the acetabulum were quantified via multiple 2D manual measurements and automated 3D measurements. There were no 2D measurements found to differ between the WB and WB-stress scans in either dysplastic (P = .742–1.000) or control (P = .203–1.000) hips. 3D translation of the femoral head center from WB to WB-stress averaged 1.3 ± 0.6 mm in the control hips, compared to 0.9 ± 0.4 mm in the dysplastic hips (P = .096). 3D joint space width (JSW) was determined for both the control and dysplastic hips, with greater JSW found in control hips for both the WB (P = .049) and WB-stress (P = .003) scans. WBCT has the potential to better capture subtle femoral–acetabular displacement derived from both automated 3D and manual 2D measurements in static instability-prone joint orientations.
Association of radiation-induced normal tissue toxicity with a high genetic risk for rheumatoid arthritis
Authors: McWilliam Alan , Marshall Deborah , Kerns Sarah L , Barnett Gillian C , Vega Ana , Kapouranis Thodori , Aguado Barrera Miguel E , Avuzzi Barbara , Azria David , Chang-Claude Jenny , Choudhury Ananya , Coedo Costa Carla , Dunning Alison , Farcy-Jacquet Marie-Pierre , Faivre-Finn Corinne , Gutiérrez-Enríquez Sara , Fuentes-Ríos Olivia , Gómez Caamaño Antonio , Lambrecht Maarten , López Pleguezuelos Carlos , Rancati Tiziana , Rattay Tim , de Ruysscher Dirk , Seibold Petra , Sperk Elena , Talbot Christopher , Webb Adam , Veldeman Liv , Rosenstein Barry S , West Catharine M L ,   REQUITE Consortium, Stegmaier P , Claßen J , Schnabel T , Müller Anusha , Helmbold Irmgard , Elliott Rebecca 
Published: January 2025

Abstract

Background
Overlapping genes are involved with rheumatoid arthritis (RA) and DNA repair pathways. Therefore, we hypothesized that patients with a high polygenic risk score for RA will have an increased risk of radiotherapy toxicity given the involvement of DNA repair.

Methods
Primary analysis was performed on 1494 prostate cancer, 483 lung cancer, and 1820 breast cancer patients assessed for development of radiotherapy toxicity in the REQUITE (validating pREdictive models and biomarkers of radiotherapy toxicity to reduce side effects and improve QUalITy of lifE in cancer survivors) study. Validation cohorts were available from the Radiogenomics Consortium. All patients had undergone curative-intent radiotherapy and were assessed prospectively for toxicity. Germline genomic data was available for all patients, allowing a polygenic risk score to be calculated using 101 RA risk variants. Polygenic risk score was analyzed as a continuous variable and with a more than 90th percentile cutoff. Associations with acute and late standardized total average toxicity (STAT) scores and individual toxicity endpoints were analyzed in multivariable models with preselected adjustment variables.

Results
Increasing polygenic risk score for RA did not increase the risk of STAT-acute or STAT-late in any cohort. There was an increased risk of late esophagitis in the lung cancer cohort (coefficient = 0.018, P = .01), however this was not validated (P = .79). No individual acute or late toxicity endpoints were statistically significantly associated with polygenic risk score for the prostate or breast cohorts. No statistically significant results were found in the validation cohorts in multivariable models.

Conclusions
Patients with a high genetic risk for RA do not show increased levels of toxicity after radiotherapy suggesting treatment planning does not need to be modified for such patients.
‘Global talent’ or ‘labour migrant’?—Discursive noise and the strategic organization of difference in the competition state
Authors: Martel Kerstin 
Published: January 2025

Abstract
This article explores how migrant categorizations are (re)produced across distinct discursive terrains in public spheres and in the academic fields of migration studies and management and organization studies. It illustrates state-economy entanglements through the emergence of novel migrant labels, such as ‘global talent’, within the empirical context of Copenhagen, Denmark, and at a European scale. The findings suggest that the use and enactment of distinct categorizations in public discourse organizes social difference strategically: performative speech acts ascribe symbolic capital and status to foreign newcomers who are of economic value to the nation state, while not granting this privilege to others. Thereby, the ideological and discursive infusion of a human capital rationale into the state’s migration regime and at supra-national instances supports not only processes of Othering, but produces ontological effects. The use of certain designations in specific situations, such as ‘global talent’ in a mayor’s welcome address or ‘expat’ in housing adverts, seems inappropriate and unsettling. To grasp the fragmentation, accumulation and disturbing repetition of labels across distinct discursive spheres, the author introduces the concept of ‘discursive noise’. The denser the discursive assemblage becomes, and the more congruent categorizations across distinct spheres seem to be, the less perceptible underlying ambiguities are. The idea of discursive noise upholds that the repetition and circulation of consistent, yet conceptually incoherent migrant designations across commercial and political spheres is deliberate and strategically intended. Discursive noise conceals ideological underpinnings of migrant triage whilst backing politico-economic goals of the competition state.
Gaussian process regression of temperature-dependent radial velocities
Authors: Rescigno Federica , Al Moulla Khaled 
Published: January 2025

ABSTRACT
Gaussian processes (GPs) described by quasi-periodic covariance functions have in recent years become a widely used tool to model the impact of stellar activity on radial velocity (RV) measurements. We perform a GP regression analysis on solar RV time series measured from spectral segments formed at different temperatures within the photosphere in order to evaluate the relation between the best-fit GP kernel hyperparameters and the observed activity signal as a function of temperature. The posterior distributions of the hyperparameters show subtle differences between high- and low-activity phases and as a function of the spectral formation temperature range, which could have implications on the characteristics of the activity signal and its optimal modelling. For the temperature-dependent RVs, we find that at high and low activity alike, the minimal RV dispersion is obtained at intermediately cool temperature ranges ($4000{-}4750 \,{\rm K}$), for both the observed and GP model-subtracted RVs. Finally, we compare and correlate our temperature-dependent RVs with RV components derived from disc-resolved Dopplergrams of the Sun, for which we find a consistently strong correlation between RVs related to hotter temperature ranges and the dominant RV component due to the inhibition of convection.
Expanding molecular shell and possible γ-ray source associated with supernova remnant kesteven 67
Authors: Shen Yun-Zhi , Chen Yang , Zhang Xiao , Tu Tian-Yu , Zhong Wen-Juan , Zhang Qian-Qian , Liu Qian-Cheng 
Published: January 2025

ABSTRACT
We investigate the molecular environment of the supernova remnant  (SNR) Kesteven 67 (G18.8+0.3) using observations in $^{12}$CO, $^{13}$CO, HCO$^+$, and HCN lines and possible associated $\gamma$-ray emission using 16-yr Fermi–LAT observation. We find that the SNR is closely surrounded by a molecular belt in the southeastern boundary, with the both recessed in the band-like molecular gas structure along the Galactic plane. The asymmetric molecular line profiles are widely present in the surrounding gas around local-standard-of-rest velocity $+20\, {\mathrm{k}\mathrm{ m}}\, {\mathrm{s}}^{-1}$. The secondary components centred at $\sim +16\, {\mathrm{k}\mathrm{ m}}\, {\mathrm{s}}^{-1}$ in the belt and $\sim +26\, {\mathrm{k}\mathrm{ m}}\, {\mathrm{s}}^{-1}$ in the northern clump can be ascribed to the motion of a wind-blown molecular shell. This explanation is supported by the position–velocity diagram along a line cutting across the remnant, which shows an arc-like pattern, suggesting an expanding gas structure. With the simulation of chemical effects of shock propagation, the abundance ratios N(HCO$^+$)/N($^{12}$CO) $\sim 2.6\times 10^{-5}$–$3.6\times 10^{-4}$ obtained in the belt can be more naturally interpreted by the wind-driven bubble shock than by the SNR shock. The belt and northern clump are very likely to be parts of an incomplete molecular shell of bubble driven by O-type progenitor star’s wind. The analysis of 0.2–500 GeV $\gamma$-ray emission uncovers a possible point source (‘Source A’) about 6.5$\sigma$ located in the north of the SNR, which essentially corresponds to northern molecular clump. Our spectral fit of the emission indicates that a hadronic origin is favoured by the measured Galactic number ratio between CR electrons and protons $\sim 0.01$.
Genome-wide CRISPR-Cas9 screens identify BCL family members as modulators of response to regorafenib in experimental glioma
Authors: Haeusser Lara Annina , Becker Hannes , Kuhlburger Laurence , Zago Marcello , Walter Bianca , Tsiami Foteini , Erdmann Sarah , Trampert Jil , Surender Surender , Stahl Aaron , Templin Markus , Wegner Eileen , Schmidt Tobias , Schmees Christian , Casadei Nicolas , Sevenich Lisa , Claassen Manfred , Nahnsen Sven , Beck Susanne , Merk Daniel Josef , Tabatabai Ghazaleh 
Published: January 2025

Abstract

Background
Registered systemic treatment options for glioblastoma patients are limited. The phase II REGOMA trial suggested an improvement of median overall survival in progressive glioblastoma by the multi-tyrosine kinase inhibitor regorafenib. This has not been confirmed by GBM AGILE. So far, regorafenib has been administered as monotherapy or as an addition to standard of care in newly diagnosed glioblastoma. Rational combination therapies involving regorafenib might be a reasonable strategy. Here, we aimed at identifying functionally instructed combination therapies involving regorafenib.

Methods
We applied a genome-wide CRISPR-Cas9-based functional genomics target discovery approach using activation and knockout screens followed by genetic, pharmacological, functional validations. Regorafenib-induced molecular alterations were assessed by RNA sequencing and DigiWest. We investigated selected functionally instructed combination therapies in three orthotopic glioma mouse models in vivo (syngeneic SMA560/VM/Dk model and two xenograft models) and performed immunohistochemistry of post-treatment brains.

Results
We identified potential modifiers of regorafenib response, including BCL2, BCL2L1, ITGB3, FOXC1, SERAC1, ARAF, and PLCE1. The combination of regorafenib with Bcl-2/Bcl-xL inhibition was superior to both monotherapies alone in vitro, ex vivo, and in vivo. We identified regorafenib-induced regulations of the Bcl-2 downstream target chemokine receptor 1 (CCR1) as one potential underlying molecular mediator. Furthermore, regorafenib led to changes in the myeloid compartment of the glioma-associated microenvironment.

Conclusions
This preclinical study uses a functional genomics-based target discovery approach with subsequent validations involving regorafenib. It serves as a biological rationale for clinical translation. Particularly, an investigation of the combination of regorafenib plus navitoclax within a clinical trial is warranted.