bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distribution, abundance and ecogenomics of the cosmopolitan Gemmatimonadota group PAUC43f
Authors: Aldeguer-Riquelme Borja , Antón Josefa , Santos Fernando 
Published: May 2022

Abstract
BackgroundThe phylum Gemmatimonadota comprises mainly uncultured microorganisms and presents a high level of diversity, similar to that of Proteobacteria or Actinobacteria. This diversity reflects the phylum’s wide range of functional capabilities, which allows its members to inhabit different environments such as soils, freshwater lakes, marine sediments, sponges or corals. Some studies have focused on the Gemmatimonadota present in soils or freshwater lakes, however, to date, the information about this group in the marine environment is very scarce. One of the most frequently retrieved Gemmatimonadota from marine environments is PAUC43f, an uncultured group that has been detected only by 16S rRNA gene sequence analyses, with not a single isolate or metagenome-assembled genome (MAG) affiliated with this group. ResultsHere, we carried out a broad study of the distribution, abundance, ecotaxonomy and potential metabolic capabilities of PAUC43f by mining information from 16S rRNA gene amplicon datasets, Gemmatimonadota MAGs, and fluorescent in situ hybridization (FISH). We detected PAUC43f in 4,965 amplicon datasets mainly from marine sediments, sponges, corals and saline soils and lakes around the world, which indicated this group is cosmopolitan and adapted to saline environments. PAUC43f represented a substantial fraction of the microbial community, mainly in sediments, sponges and soils where, in some cases, it could account for up to 34.3% of the 16S rRNA gene sequences. The potential metabolic capabilities inferred from the 39 PAUC43f MAGs suggested a facultatively aerobic and chemoorganotrophic metabolism, although some members could also oxidise hydrogen. Furthermore, PAUC43f could play a key environmental role as a N2O consumer as well as a supplier of cysteine, serine and thiamine. Finally, the design and test of a FISH probe provided experimental evidence of the presence, activity, and morphology of PAUC43f in marine sediments. We propose its renaming as “Candidatus Palaucibacterales”, in reference to the Republic of Palau where the first sequence of this group was recovered, and its classification as a new order within the Gemmatimonadota phylum and Gemmatimonadetes class.ConclusionsOur findings highlight the global distribution and the potentially relevant ecological roles of PAUC43f in different worldwide distributed environments, mainly as N2O scavengers as well as vitamin and amino acid suppliers.
Kinetic energy and fluence measurements correlated with self- generated electric and magnetic fields of spatially resolved laser ablated Zr-plasma at different irradiances
Authors: Javed Mubashir , Bashir Shazia , Akram Mahreen , Mahmood Khaliq , Ayub Rana , Hussain Fida , Fatma Nazli , Iqbal Muqaddas 
Published: May 2022

Abstract
Experimental investigations on spatially- resolved- laser-generated Zr-plasma parameters and their relationship with the Self-Generated- Electric and Magnetic- Fields (SGEMFs) at different irradiances have been performed. The Faraday Cup (FC) is employed to measure plasma species' kinetic energy (K.E) and fluence, while electric and magnetic probes are used to measure SGEMFs of Zr-plasma. The decreasing trends for evaluated values of plasma parameters and SGEMFs have been found with increasing FC axial distance from the target, whereas, increasing trends with increasing laser irradiance are observed. The kinetic energy of Zr ions increases from 1.8 keV to 11.8 keV, whereas, fluence increases from 1×1013cm-2 to 2×1013cm-2 by increasing the laser irradiance. Similarly, the electric field of Zr plasma increases from 107Vm-1 to 237Vm-1 and magnetic field increases from 235G to 590G. The generation of SGEF is attributed to charge-separation and double-layer-structure whereas, SGMF is attributed to anisotropic temperature (∇𝑇𝑒) and density (∇𝑛𝑒) gradients of plasma.
Transferred Local Fisher Discriminant Analysis
Authors: Zandifar Mozhdeh , Rezaie Samaneh , Tahmoresnezhad Jafar 
Published: May 2022

Abstract
Domain adaptation in machine learning and image processing aims to benefit from gained knowledge of the multiple labeled training sets (i.e. source domain) to classify the unseen test set (i.e. target domain). Therefore, the major issue emerges from dataset bias where the source and target domains have different distributions. In this paper, we introduce a novel unsupervised domain adaptation method for cross-domain visual classification. We suggest a unified framework that reduces both statistically and geometrically shift across domains, referred to as Transferred Local Fisher Discriminant Analysis (TLFDA). Specifically, TLFDA projects data into a shared subspace to minimize the distribution shift between domains and simultaneously preserves the discrimination across different classes. TLFDA maximizes the between-class separability and preserves the within-class local structure in form of an objective function metrics. The objective function is solved effectively in closed-form. Broad experiments demonstrate that TLFDA significantly outperforms many state-of-the-art domain adaptation methods on different cross-domain visual classification tasks.
Association of Adverse Childhood Experiences with Oral Health Status of children living in Rural Areas of Alexandria, Egypt: A household Survey
Authors: Moussa Hana , Rahman Ahmed Abdel , Esam Wafaa 
Published: May 2022

Abstract
Background: Scarce research assessed the link between Adverse childhood experiences and oral health status of Egyptian children whose level of oral diseases and Adverse childhood experiences may be different from western countries and who have high level of caries. This study aims to investigate the association between ACE and the oral health status of children living in rural areas in Alexandria, Egypt.Methods: A cross-sectional household survey was carried in North Western Delta, including 300 children 6-18 years old. Children were examined for dental caries and gingival condition then questioned about their oral hygiene habits and sugar consumption. Mothers/caregivers answered the adverse childhood experiences questionnaire. Three linear regression models were used to assess the relationship between dependent variables (primary caries experience, permanent caries experience, gingival score) and exposure (ACE) after adjusting for potential confounders. Furthermore, another 3 models were used to evaluate oral health behaviors (sugar consumption, plaque consumption, dental visits) effect on dependent variables. Results: Most children were females (57.2%), mean age was 9.81, SD= 3.06. 68.6% children had caries experience in their primary teeth (mean ± SD dft= 3.03± 3.14) and 27.9% had caries experience in their permanent teeth (mean ± SD DMFT= 0.60 ± 1.16) with mean ± SD gingival index score 1.14± 0.37. The mean ± SD ACE score was 4.15 ± 1.91. When comparing adjusted R2 of ACE models and oral health behaviors models; dft model of both had an identical value of 0.44. However, adjusted R2 of ACE models (DMFT =0.20, and gingival score =0.03) were different than those in oral health behaviors models (DMFT =0.22, gingival score =0.45).Conclusions: ACEs explained the same amount of variation in primary caries experience as sugar consumption, plaque accumulation and dental visits. Yet, it doesn’t have the same effect on permanent caries experience or the gingival condition. Therefore, ACEs might have the same effect on primary dental caries as oral health behaviors. Further research is needed to evaluate if a dose-response relationship is present between ACEs, oral health and oral health behaviors as suggested by previous research.
Do They Suffer More? Evidence from the Association between Short-term Exposure to PM10 and Cardiovascular Hospital Admissions in Persons with Disabilities
Authors: Kim Sera , Lee Jong-Tae 
Published: May 2022

Abstract
Background: Persons with disabilities (PwD), the world’s largest minority, can be more susceptible to particulate matter (PM) than persons without disabilities. Although numerous studies have addressed the population susceptible to PM, PwD have not been studied in air pollution epidemiology. This study investigated the association between short-term exposure to PM with an aerodynamic diameter smaller than 10 μm (PM10) and cardiovascular hospital admissions by the existence of a disability, while also considering individual-level effect modifiers (disability and socio-demographic characteristics) in South Korea.Methods: We used the National Health Insurance Service-National Sample Cohort (NHIS-NSC) to investigate the association between short-term exposure to PM10 and cardiovascular hospital admissions in seven metropolitan cities from 2002 to 2015. We conducted a time-stratified case-crossover analysis using conditional logistic regression and adjusted for daily temperature, relative humidity, air pressure, and national holidays. We conducted stratified analyses according to the existence of a disability, disability type and severity, and socio-demographic characteristics.Results: A 10 μg/m3 increase in the 0-3 moving average level of PM10 was associated with 1.9% (95% confidence interval [CI]: 0.7%, 3.2%) and 0.0% (95% CI: -0.5%, 0.5%) increase in cardiovascular admissions in persons with and without disabilities, respectively. Among PwD, the associations were pronounced in people with brain lesion disorders (percent change [PC]: 2.7%, 95% CI: 0.5%, 5.0%), people with visual impairment (PC: 3.0%, 95% CI: -1.0%, 7.1%), and people with severe disability (PC: 3.0%, 95% CI: 0.9%, 5.0%).Conclusions: We found that PwD may be more adversely affected by PM10 than their non-disabled counterparts. This suggests that PwD is a social identity reflecting the socially marginalized and disadvantaged population in air pollution epidemiology.
The earliest vascular land plants from the Upper Ordovician of China
Authors: Xu Honghe , Wang Kai , Huang Zhengliang , Tang Peng , Wang Yi , Liu Bingcai , Yan Wei 
Published: May 2022

Abstract
Land plants (embryophytes) on the Earth were inferred to originate in the Cambrian Period (Morris et al., 2018; Donoghue et al., 2021) and to evolve from algae through nonvascular forms to vascular plants. This evolutionary scenario is verified by progressive fossil records ranging from Middle Ordovician embryophyte-derived cryptospores (Strother et al., 1996, 2015) and spore-containing fragments (Wellman et al., 2003), to vascular plant debris, including Late Ordovician trilete spores (Rubinstein and Vajda, 2019; Steemans et al., 2009), branched axes, and late Silurian tracheids and stomata (Kenrick et al., 2012). Among these key characters of vascular land plants, tracheid crucially distinguishes them from nonvascular groups, yet its fossil record is much later than estimated origination time, and vascular plant sporophytes had fossil records till the Wenlock (c. 430 Ma) of the Silurian Period (Libertín et al., 2018). Here we demonstrate the earliest bona fide vascular plants by specific meso- and microfossils from the Upper Ordovician (early Sandbian, c. 458 Ma) of the Ordos Basin, Inner Mongolia, China. Our fossils include bifurcated axes up to 687 μm wide, stomata-bearing epidermis, laevigate trilete spores, and well-developed tracheids with distinct lateral wall facets. Combined with the small stature of these plants, our finds show an evolutionarily transitional link from pre-Darriwilian, cryptospore-producing, and gametophyte-dominant plants to crown tracheophytes. Late Ordovician might witnesses the first explosion of vascular land plants and there had been a widespread flora of both bryophyte-like and small vascular plants dwelling the seaside by the Late Ordovician Epoch, than which the terrestrialization of vascular plants started much earlier.
Administration of Granulocyte-macrophage colony-stimulating factor enhanced CAR T-cell expansion and cellular immunity recovery without inducing cytokine release syndrome: a retrospective study
Authors: Jiang Ying , Feng Dan , Wang Chun , Zhang Yanlei , Zhao Chuxian , Li Su , Qin Youwen , Chang Alex H. , Zhu Jun 
Published: May 2022

Abstract
Background: Leukopenia and cytokine release syndrome (CRS) are two major toxicities of CAR-T cell therapy. GM-CSF is an ideal candidate treatment for leukopenia except for its potential aggravation of CRS. We hypothesized that the optimal timing of supplemental with GM-CSF in a shortage of host immunity and CAR T-cell was chosen as avoidance of CRS. In the study we evaluated the safety and efficacy of GM-CSF intervention post-CAR T-cell therapy while circulating CAR T-cell declined. Methods: Nine patients received GM-CSF therapy who displayed moderate leukopenia with white blood cell counts (WBC) <3000 cells/mm3 and absolute neutrophil counts (ANC) <1500 cells/mm3 with concomitant declination of circulating CAR T-cell after CAR T-cell therapy. Results: The median duration of GM-CSF intervention was 15 days (4-30). CAR T-cell expansion was observed in eight patients (8/9), including cerebrospinal fluid of one patient and peripheral blood (PB) of other seven patients. And the peaks of CAR T-cell levels in cerebrospinal fluid and PB appeared in day 6 and day 7 (2-11) following the initiation of GM-CSF administration. Also, increased white blood cells in PB was observed in all patients. The median onset and duration time of WBC recovery were 9 (1-14) and 17(3-53) days. Moreover, the increment of WBC, neutrophil, lymphocyte and CD3-CD16+CD56+ natural killer cell in PB was observed. In addition, no CRS or fatal infection occurred during GM-CSF treatment. Conclusion: This study provides evidence for the clinical feasibility of combining CAR T-cell therapy with the GM-CSF to treat leukopenia patients with concomitant declination of circulating CAR T-cell.
A new superposition between lump, breather and line waves of the (2+1)-dimensional generalized Korteweg–de Vries equation
Authors: ma hongcai , Yue Shupan , Deng Aiping 
Published: May 2022

Abstract
The work in this paper focuses on a new superposition between a single lump wave, breather waves and line solitons of the (2+1)-dimensional generalized Korteweg–de Vries(gKdV) equation. After deriving the N-soliton solutions, a new constraint, the long-wave limit and mode resonance method are used to construct a new hybrid solution. The hybrid solution consists of a single lump, breather waves and line solitons, where multiple line solitons and multiple breather waves are never collided by a single lump wave, or where at least one soliton in the breather waves and line solitons is always covered by a single lump wave. Further, a new velocity resonance condition is proposed to obtain a velocity resonance solution consisting of a single lump wave, one line soliton and breather waves, which remain relatively stationary and form a new bound state. Particularly, we find that two breather waves are generated when a line soliton and a breather wave touched. It is worth pointing out that in most of the previous literature, lump wave and other waves will separate after they collide, but this paper derives a different result. In this paper, other waves are never collided by lump wave or are always covered by lump wave, which means that these waves either never collide, or do not separate once they meet.
Overexpression of TRIM44 mediates the NF-kB pathway to promote OC progression and metastasis
Authors: Yu Yang , Li Shi ying , Xie Liang-Zhen , Guo Ying , Li Jia , Shen Ying , Fu Yang , Han Feng-Juan 
Published: May 2022

Abstract
Background: TRIM44 is highly expressed in both ovarian cancer tissues and cells, and is significantly associated with clinicopathological factors and poor prognosis. However, its effect on the malignant biological behavior of ovarian cancer cells and its potential mechanism of action have not been revealed. Therefore, we aimed to investigate the role and potential mechanism of TRIM44 on ovarian cancer.Methods: Using shRNA transfection to overexpress TRIM44, we investigated the effects of TRIM44 overexpression on the proliferation, migration, invasion and apoptosis of ovarian cancer cells and the growth ability of xenograft tumors through in vivo xenograft mouse models and in vitro experiments. In addition, to further elucidate its molecular mechanisms of action affecting ovarian cancer progression and metastasis.Results: TRIM44 overexpression significantly promoted the proliferation, migration, and invasion of SKOV3 cells and inhibited apoptosis in vitro while promoting tumor growth in vivo. Mechanistic studies have shown that TRIM44 overexpression contributes to ovarian cancer cells invasion, metastasis, and anti-apoptosis by upregulating the levels of inflammatory cytokines (TNF-α, IL-1β, IL-8) in the tumor microenvironment, stimulating the secretion of IKKβ factors, further promoting IKBα degradation and phosphorylation, initiating NF-kB p65 entry into the nucleus, and activating the NF-kB signaling pathway to upregulate or downregulate the expression of Metallo-matrix protease (MMP-9), and apoptotic genes (Bcl-2, Caspase-3).Conclusions: These data suggest that TRIM44 overexpression promotes ovarian cancer progression and metastasis by promoting NF-kB signaling pathway activation. These results provide evidence for TRIM44 as an oncogene in ovarian cancer and suggest that it may be a potential target for ovarian cancer therapy.
Simultaneous opening-wedge distal tubercle osteotomy and double-bundle anterior cruciate ligament reconstruction for anterior cruciate ligament-deficient knees with medial compartment osteoarthritis: technical tip
Authors: Kawai Ryosuke , Kawashima Itaru , Tsukahara Takashi , Kusaka Yoshiaki 
Published: May 2022

Abstract
The surgical outcomes of simultaneous anterior cruciate ligament reconstruction (ACLR) and high tibial osteotomy (HTO) are good in ACL-deficient knees with medial compartment osteoarthritis (OA). However, this procedure has issues to be solved, such as mild knee instability and progression of OA after surgery. Herein, we introduce our surgical procedure and technical tip of opening-wedge distal tubercle osteotomy (OWDTO) performed simultaneously with anatomical double-bundle ACLR (DBACLR). This procedure aims to achieve long-term knee stability by anatomically reconstructing the ACL using the double-bundle technique even though ACLR and OWDTO are both performed for ACL-deficient knees with medial compartment OA.
Integrated miRNA-mRNA analysis revealed distinct molecular signatures and regulatory networks in cancer stem-like cell models of hepatocellular carcinoma
Authors: Ayob Ain Zubaidah , Said Nur Akmarina Mohd , Ramasamy Thamil Selvee 
Published: May 2022

Abstract
Purpose: Tumour micro-niche and cancer therapeutics have been implicated in cancer stem cells (CSCs) enrichment, contributing to cancer heterogeneity, aggressiveness, and resistance, eventually leading to metastasis and tumour relapse. We aim to clarify how these cellular pressures differentially influence the enrichment of CSC-like cells and elucidate the molecular regulatory networks underlying distinct CSCs. Method: Three HepG2-derived CSC-like models were established to recapitulate CSC induction from different pressures including i) tumoursphere (3D-HepG2), ii) TGF-β1-induced epithelial-mesenchymal transition (EMT; TβT-HepG2) and iii) cisplatin-resistant (CisR-HepG2) models. These models were then subjected to an integrated miRNA and mRNA profiling and molecular regulatory network analysis. Results: All models exhibited enrichment of aldehyde dehydrogenase positive cells with differential upregulations of CD133 and distinct EpCAM species upregulated between different models. These models shared common propensity for increased stemness, quiescence, invasion, migration, and chemoresistance, while proliferation and EMT features were differential. Profiling analysis revealed the differentially expressed miRNA and mRNA of the 3D-HepG2 clustered closely to the parental, whereas distinct profiles were displayed by the TβT-HepG2 and CisR-HepG2. Seven miRNAs (miR-19b-3p miR-23a-3p, miR-29a-3p, miR-92a-3p, 125a-5p, miR-181a-5p, miR-483-3p) and 24 mRNAs - among which include CCNB1, PI3KR5, MAP2K6, MAPK10 and MAPK11, were identified as key drivers underlying the molecular regulatory networks of these CSC-like models. Notably, the integrated analysis revealed the FoxO/PI3K-Akt/MAPK signalling and regulation G2/M transition and mitotic spindle checkpoints of cell cycle were identified as common network through the regulation of the key drivers.Conclusion: Altogether, the molecular regulatory networks from the three CSC-like models provide insights into the mechanisms regulating cancer plasticity and heterogeneity that may potentially be harnessed for the development of improved therapeutic approaches in targeting CSC for cancer treatment.
Vision-related Quality of Life After Cataract Surgery in West Africa
Authors: Signes-Soler Isabel , Javaloy Jaime , Montés-Micó Robert , Muñoz Gonzalo , Llamusí Raúl Montalbán , Baeza Ana Maria Hernandez , Albarrán-Diego César 
Published: May 2022

Abstract
AIMSTo study the vision related quality of life (VRQoL) before and after a mass cataract surgery campaign in a low-income country and to describe the impact of cataract surgery on quality of life (QOL). METHODSAll the patients who had cataract surgery in Bobo-Dioulasso (Burkina Faso) during a campaign for the prevention blindness were examined. VRQoL was assessed using a modified version of the WHO/PBD VF20. The questionnaire was carried out before and three months after the surgery. A QOL related to vision index (QOL-RVI) was calculated from the scores obtained.RESULTSA total of 305 patients (56.1% male) underwent cataract extraction. Mean age was 61.97 ± 14.39 years. The majority of eyes (88.70%) had preoperatively poor visual acuity (CDVA<20/200). Three months after surgeries, good visual outcomes (VA ≥20/60) were achieved in 38.35% of eyes without correction and 68.7% with correction. At this time, QOL-RVI had improved postoperatively in 90.2% of patients, had not changed in 3.1% and was worse in 6.7%. Significant differences were detected (Wilcoxon test p <0.05) for all items tested before and after surgeries. Correlations for the patients after operations showed that there was a significant relation between a global estimated QOL-RVI and the UCVA previous to the surgery (ρ -0.196 p=0.014), regarding the same index and the BCVA after the procedures (ρ -0.35 p=0.00018). CONCLUSIONSCataract surgery increases the quality of life of patients living in low-income countries such as Burkina Faso and the improvement correlates with the recovery of the visual acuity.