bluuebunny/crossref_metadata_2025_split huggingface dataset:
QTL mapping and identification of candidate genes using a genome-wide association study for heat tolerance at anthesis in rice (Oryza sativa L.)
Authors: Hu Changmin , Jiang Jianhua , Li Yulong , Song Shaojie , Zou Yu , Jing Chunyu , Zhang Ying , Wang Dezheng , He Qiang , Dang Xiaojing 
Published: September 2022

Heat tolerance (HT) of rice at anthesis is a key trait that ensures high and stable yields under heat stress. Finding the quantitative trait loci (QTLs) and gene loci controlling HT is crucial. We used relative spikelet fertility (RSF) as a measure of HT. The phenotypic values of RSF in 173 rice accessions were investigated in two environments and showed abundant variations. We performed a genome-wide association study on RSF using 1.2 million single nucleotide polymorphisms (SNPs). Five QTLs were significantly associated with RSF were identified, four were found in previously reported QTLs/genes, and one was novel. The novel QTL qRSF9.2 was mapped into the 22,059,984-22,259,984 bp region, which had 38 positional candidate genes. By combining the linkage disequilibrium analysis, the QTL region was narrowed to 22,110,508–22,187,677 bp, which contained 16 candidate genes. Among them, only gene LOC_Os09g38500 contained nonsynonymous SNPs that were significantly associated with RSF. In addition, accessions with large and small RSF values had corresponding respective high and low gene expression levels. Furthermore, the RSF of the CC allele was significantly higher than that of the TT allele. Hap 2 and Hap 3 can increase heat tolerance by 7.9 and 11.3%, respectively. Our results provide useful information that recommends further cloning of qRSF9.2 and breeding heat-tolerant rice varieties by marker-assisted selection.
m6A methylation regulators as predictors for treatment of advanced urothelial carcinoma with anti-PDL1 agent
Authors: Kong Jianqiu , Lu Sihong , Zhang Long , Yao Yuhui , Zhang Jie , Shen Zefeng , Luo Mingli , Liu Bin , Zheng Junjiong , Lin Tianxin 
Published: September 2022

PurposeImmune checkpoint blockade agents were shown to provide a survival advantage in urothelial carcinoma, while some patients got minimal benefit or side effects. Therefore, we aimed to investigate the prognostic value of m6A methylation regulators, and developed a nomogram for predicting the response to atezolizumab in urothelial carcinoma patients.MethodsA total of 298 advanced urothelial carcinoma patients with response data in the IMvigor210 cohort were included. Differential expressions of 23 m6A methylation regulators in different treatment outcomes were conducted. Subsequently, a gene signature was developed in the training set using the least absolute shrinkage and selection operator (LASSO) regression. Based on the multivariable logistic regression, a nomogram was constructed by incorporating the gene signature and independent clinicopathological predictors. The performance of the nomogram was assessed by its discrimination, calibration, and clinical utility with internal validation.ResultsSix m6A methylation regulators, including IGF2BP1, IGF2BP3, YTHDF2, HNRNPA2B1, FMR1, and FTO, were significantly differentially expressed between the responders and non-responders. These six regulators were also significantly correlated with the treatment outcomes. Based on the LASSO regression analysis, the gene signature consisting of two selected m6A methylation regulators (FMR1 and HNRNPA2B1) was constructed and showed favorable discrimination. The nomogram integrating the gene signature, TMB, and PD-L1 expression on immune cells, showed favorable calibration and discrimination in the training set (AUC 0.768), which was confirmed in the validation set (AUC 0.755). Decision curve analysis confirmed the potential clinical usefulness of the nomogram.ConclusionsThis study confirmed the prognostic value of FMR1 and HNRNPA2B1, and constructed a nomogram for individualized prediction of the response to atezolizumab in patients with urothelial carcinoma, which may aid in making treatment strategies.
Association between SII and hepatic steatosis and liver fibrosis: A population-based study
Authors: Xie Ruijie , Xiao Mengde , Li Lihong , Ma Nengqian , Liu Mingjiang , Huang Xiongjie , Liu Qianlong , Zhang Ya 
Published: September 2022

BackgroundThe systemic immune-inflammation index (SII) is a novel marker of inflammation, and hepatic steatosis and fibrosis are associated with inflammation. This study aimed to investigate the possible relationship between SII and hepatic steatosis and fibrosis.MethodsThe datasets from the National Health and Nutrition Examination Survey (NHANES) 2017–2020 were used in a cross-sectional investigation. Multivariate linear regression models were used to examine the linear connection between SII and controlled attenuation parameter (CAP) and liver stiffness measurement (LSM). Fitted smoothing curves and threshold effect analysis were used to describe the nonlinear relationship.ResultsThis population-based study included a total of 6,792 adults aged 18–80 years. In a multivariate linear regression analysis, a significant positive association between SII and CAP was shown [0.006 (0.001, 0.010)]. This positive association in a subgroup analysis was maintained in men [0.011 (0.004, 0.018)] but not in women. Furthermore, the association between SII and CAP was nonlinear; using a two-segment linear regression model, we found an inverted U-shaped relationship between SII and CAP with an inflection point of 687.059 (1,000 cells/µl). The results of the multiple regression analysis showed that the relationship between SII and LSM was not significant (P = 0.263).ConclusionsOur findings imply that increased SII levels are linked to hepatic steatosis, but SII is not linked to liver fibrosis. To confirm our findings, more large-scale prospective investigations are needed.
Instantaneous death risk, conditional survival and optimal surgery timing in cervical fracture patients with ankylosing spondylitis: A national multicentre retrospective study
Authors: Huang Jinfeng , Bai Hao , Tan Quanchang , Hao Dingjun , Wu Aimin , Wang Qingde , Wang Bing , Wang Linfeng , Liu Hao , Chen Xiongsheng , Jiang Zhengsong , Ma Xiaoming , Liu Xinyu , Liu Peng , Cai Weihua , Lu Ming , Mao Ningfang , Wang Yong , Fu Suochao , Zhao Shuai , Zang Xiaofang , Xie Youzhuan , Yu Haiyang , Song Ruixian , Sun Jiangbo , Xiang Liangbi , Liu Xiang , Li Songkai , Liao Bo , Wu Zixiang 
Published: September 2022

BackgroundThe mortality rate in patients with ankylosing spondylitis (AS) and cervical fracture is relatively high.ObjectivesThis study aimed to investigate the instantaneous death risk and conditional survival (CS) in patients with AS and cervical fracture. We also studied the relationship between surgical timing and the incidence of complications.MethodsThis national multicentre retrospective study included 459 patients with AS and cervical fractures between 2003 and 2019. The hazard function was used to determine the risk of instantaneous death. The five-year CS was calculated to show the dynamic changes in prognosis.ResultsThe instantaneous death risk was relatively high in the first 6 months and gradually decreased over time in patients with AS and cervical fracture. For patients who did not undergo surgery, the instantaneous risk of death was relatively high in the first 15 months and gradually decreased over time. For patients with American Spinal Injury Association impairment scale (ASIA) A and B, the 5-year CS was 55.3% at baseline, and improved steadily to 88.4% at 2 years. Odds ratios (ORs) for pneumonia, electrolyte disturbance, respiratory insufficiency, and phlebothrombosis decreased as the surgery timing increased.ConclusionDeaths occurred mainly in the first 6 months after injury and gradually decreased over time. Our study highlights the need for continued surveillance and care in patients with AS with cervical fractures and provides useful survival estimates for both surgeons and patients. We also observed that early surgery can significantly increase functional recovery, and decrease the incidence of complications and rehospitalisation.
Radiotherapy improves the outcomes of immunotherapy with Sintilimab in non-small-cell lung cancer: A real-world analysis
Authors: Li Shuling , Chen Kuifei , Yang Meiwen , Hlaing Swe Swe , Chen Meng , Gu Pinjun , Meng Yinnan , Yang Haihua 
Published: September 2022

IntroductionRadiotherapy may augment systemic antitumor responses to immunotherapy. We did a retrospective study to infer whether radiotherapy improves outcomes to immunotherapy in patients with stage III and IV non-small-cell lung cancer (NSCLC).MethodsThis retrospective study conducted at Enze Medical Center enrolled 259 patients with histopathology confirmed NSCLC from December 2018 to December 31, 2021. All were treated with Sintilimab, some patients received radiotherapy at an appropriate time point. Radiation type includes conventional radiotherapy and stereotactic body radiotherapy. The progression-free survival (PFS), and overall survival (OS) were the primary endpoint.ResultsA retrospective analysis was performed on 259 patients, of whom 140 had been treated with immunotherapy lonely and 119 had been remedied with immunotherapy plus radiotherapy. Baseline variables were well balanced between the two groups, including gender, age, smoking status, TNM staging, number of metastases, ECOG score, pathological type and lines of previous systemic therapy. The median PFS in the immunotherapy alone group was 5.00 months (95%CI 4.38-5.62) versus immunotherapy plus radiotherapy was 9.00 months (5.95-12.05; p&lt;0.001). The median OS in the immunotherapy alone group was 16.00 months (12.59-19.42) versus immunotherapy plus radiotherapy was 30.00 months (20.75-39.25; p=0.027). PFS was finer in the radiotherapy plus immunotherapy group than the immunotherapy group alone in both stage III(P=0.0069) and Stage IV(P=0.006) patients. In the univariate analysis, radiotherapy, male, ECOG=0 and &lt;2 lines of previous systemic therapy were connected with an observably better PFS (P&lt;0.001; P=0.03; P=0.002;P=0.021). In a multivariate analysis, radiotherapy, ECOG=0 and &lt;2 lines of previous systemic therapy were independent prognostic factors with a markedly better PFS (P&lt;0.001; P=0.006;P=0.009). An univariate analysis, radiotherapy, male, stage III, non-metastasis, ECOG=0 and squamous carcinoma were associated with a significantly better OS (P=0.032, P=0.036,P=0.002,P&lt;0.001,P=0.002,P=0.025). A multivariate analysis, non-metastasis was a standalone prognostic indicator with a significantly better OS (P=0.006). However, radiotherapy was a tendency indicator with a better OS (HR0.70 95% CI 0.47-1.06). There were also no obvious increases in adverse events in the combination group.ConclusionsRadiotherapy with addition of immunotherapy was observably linked to a better outcome in patients with III and IV staging NSCLC.
In-situ electro-stimulation enhanced branching but not massive scleractinian coral growth
Authors: Samidon Munandar , Razi Nanda Muhammad , Agustiar Muhammad , Harahap Puad Batari , Najmi Nurul , Bahri Samsul , Liu Shang Yin Vanson 
Published: September 2022

Calcium carbonate (CaCO3) is the main component of the skeleton of scleractinian corals and is a preferable substrate for attachment and growth. An electro-stimulation method based on the electrolysis of seawater, which generates calcium and magnesium minerals as a crystalline coating over artificial metal structures, was used to boost coral growth during the 1970s. Few studies have attempted to compare survival and growth between electro-stimulated corals and non-electro-stimulated corals in situ. In this study, we used 2D image analysis to quantify the growth of branching and massive corals on electro-stimulated structures. Among four metallic structures that were deployed in Sabang, Aceh, Indonesia, three were electro-stimulated (12 V) and one was used as a control. Two branching corals (Acropora gemmifera and Pocillopora verrucosa) and two massive coral species (Porites murrayensis and Porites lobata) were selected. A total of 256 fragments, comprising 128 fragments derived from two branching species and 128 fragments derived from two massive species, were randomly dispatched to each structure. Underwater photographs were taken at three intervals from August 2019 to December 2020. The results showed that the survival rates (excluding lost fragments) of branching corals under electro-stimulation were approximately 19% higher than massive. However, no significant difference was found between the survival rates of the treatment and control groups for both branching and massive corals. Furthermore, we found that under electro-stimulation, branching corals grew faster than controls but not massive. Our study provides pieces of evidence for the potential use of electrochemical processes in stimulating the growth of branching corals.
Study of performance of aerostat envelope materials on the coast
Authors: Lv Jing , Zhou Yan , Zhang Yuanping , Nie Ying , Wang Qian 
Published: September 2022

In this article, the performance of several naturally aged envelope materials in Hainan, China, was studied. The results show that the outer layer contributes to the properties of the envelope materials, where PVF and PVDF are better candidates for a weathering layer than TPU and aluminum. Due to the superior water and mildew resistance of PVF and PVDF, the loss of tensile strength for 3216LV and FV1175 was slight, whose value reduced from 101.43 ± 5.45 Kgf/cm and 76.15 ± 3.81 Kgf/cm to 79.73 ± 6.40 Kgf/cm and 70.20 ± 6.57 Kgf/cm, respectively, when aging for 24 months with the helium permeability reduction from 0.8818 L/m2•day•atm and 0.6360 L/m2•day•atm to 0.2047 L/m2•day•atm and 0.2169 L/m2•day•atm aging for 18 months, in the case of FV1175 showed leakage after aging for 24 months due to delamination. However, the tensile strength of HV150 dropped from 82.13 ± 3.61 Kgf/cm to 15.22 ± 2.16 Kgf/cm at 24 months with helium leakage, owing to heavy mildew and degradation. Meanwhile, ETAV150 also exhibited a decreased tensile strength of 57.92 ± 1.32 Kgf/cm after 6 months aging, 32.8% lower than that of the original sample, and showed leakage when aging for 12 months. This research provides a theoretical guidance for the structural design and screen of envelope materials for aerostat used along the coast.
Bibliometric-analysis visualization and review of non-invasive methods for monitoring and managing the portal hypertension
Authors: Sun XiaoHan , Ni Hong Bo , Xue Jian , Wang Shuai , Aljbri Afaf , Wang Liuchun , Ren Tian Hang , Li Xiao , Niu Meng 
Published: September 2022

BackgroundPortal hypertension monitoring is important throughout the natural course of cirrhosis. Hepatic venous pressure gradient (HVPG), regarded as the golden standard, is limited by invasiveness and technical difficulties. Portal hypertension is increasingly being assessed non-invasively, and hematological indices, imaging data, and statistical or computational models are studied to surrogate HVPG. This paper discusses the existing non-invasive methods based on measurement principles and reviews the methodological developments in the last 20 years.MethodsFirst, we used VOSviewer to learn the architecture of this field. The publications about the non-invasive assessment of portal hypertension were retrieved from the Web of Science Core Collection (WoSCC). VOSviewer 1.6.17.0 was used to analyze and visualize these publications, including the annual trend, the study hotspots, the significant articles, authors, journals, and organizations in this field. Next, according to the cluster analysis result of the keywords, we further retrieved and classified the related studies to discuss.ResultsA total of 1,088 articles or review articles about our topic were retrieved from WoSCC. From 2000 to 2022, the number of publications is generally growing. “World Journal of Gastroenterology” published the most articles (n = 43), while “Journal of Hepatology” had the highest citations. “Liver fibrosis” published in 2005 was the most influential manuscript. Among the 20,558 cited references of 1,088 retrieved manuscripts, the most cited was a study on liver stiffness measurement from 2007. The highest-yielding country was the United States, followed by China and Italy. “Berzigotti, Annalisa” was the most prolific author and had the most cooperation partners. Four study directions emerged from the keyword clustering: (1) the evaluation based on fibrosis; (2) the evaluation based on hemodynamic factors; (3) the evaluation through elastography; and (4) the evaluation of variceal bleeding.ConclusionThe non-invasive assessment of portal hypertension is mainly based on two principles: fibrosis and hemodynamics. Liver fibrosis is the major initiator of cirrhotic PH, while hemodynamic factors reflect secondary alteration of splanchnic blood flow. Blood tests, US (including DUS and CEUS), CT, and magnetic resonance imaging (MRI) support the non-invasive assessment of PH by providing both hemodynamic and fibrotic information. Elastography, mainly USE, is the most important method of PH monitoring.
Long-term trends of alanine aminotransferase levels among persons living with human immunodeficiency virus/hepatitis B virus with and without hepatitis delta coinfection
Authors: Begré Lorin , Béguelin Charles , Boyd Anders , Peters Lars , Rockstroh Jürgen , Günthard Huldrych F. , Bernasconi Enos , Cavassini Matthias , Lacombe Karine , Mocroft Amanda , Wandeler Gilles , Rauch Andri 
Published: September 2022

BackgroundHepatitis delta virus (HDV) infection accelerates the progression of liver disease in persons living with HIV and hepatitis B virus (HBV) coinfection. We explored the association between HDV infection and alanine aminotransferase (ALT) elevation during tenofovir-containing antiretroviral treatment among persons living with HIV/HBV.Materials and methodsWe included persons living with HIV/HBV with and without HDV starting tenofovir-containing antiretroviral therapy (ART) in three European cohorts with at least 18 months of follow-up. We defined HDV infection as a positive anti-HDV antibody test. We assessed risk factors for ALT elevation ≥ 1.25x upper limit of normal after 5 years of tenofovir-treatment using multivariate logistic regression models. The difference in ALT trends between individuals with and without HDV was evaluated using linear mixed effects models.Results61/518 (11.8%) participants had an HDV infection. Among individuals with HDV, 63.9% had ALT elevation after 2 years and 55.6% after 5 years of tenofovir, whereas the estimates were 34.1% after two and 27.0% after 5 years in those without HDV. HDV coinfection (adjusted odds ratio 2.8, 95% confidence interval 1.4–5.8) and obesity at baseline (adjusted odds ratio 3.2, 95% confidence interval 1.2–8.0) were associated with ALT elevation after 5 years of tenofovir therapy. Mean ALT levels were consistently higher during follow-up in participants with HDV compared to those without HDV.ConclusionPersistent ALT elevation is common in persons living with HIV/HBV in Europe despite adequate HBV therapy. HDV coinfection and obesity are independent risk factors for persistent ALT elevation during long-term tenofovir treatment.
The long-term trend of Parkinson’s disease incidence and mortality in China and a Bayesian projection from 2020 to 2030
Authors: Chen Fangyao , Chen Shiyu , Si Aima , Luo Yaqi , Hu Weiwei , Zhang Yuxiang , Ma Jiaojiao 
Published: September 2022

Background: Parkinson’s disease is a disabling degenerative disease of the central nervous system that occurs mainly in elderly people. The changes in the incidence and mortality of Parkinson’s disease at the national level in China over the past three decades have not been fully explored.Methods: Research data were obtained from the Global Burden of Disease 2019 study. The trends of crude and age-standardized incidence and mortality rates by gender of Parkinson’s disease in China were analyzed with the age-period-cohort model and the Joinpoint regression analysis. The effects of age, time period, and birth cohort on the incidence and mortality of Parkinson’s disease were estimated. The gender- and age-specific incidence and mortality rates of Parkinson’s disease from 2020 to 2030 were projected using the Bayesian age-period-cohort model with integrated nested Laplace approximations.Results: From 1990 to 2019, the annual percentage change of the age-standardized incidence rate was 0.8% (95% CI: 0.7%–0.8%) for males and 0.2% (95% CI, 0.2–0.2%) for females. And the age-standardized mortality rate for males was 2.9% (95% CI: 2.6%–3.2%) and 1.8% (95% CI: 1.5%–2.1%) for females. The results of the age-period-cohort analysis suggested that the risk and burden of Parkinson’s disease continued to increase for the last several decades. Projection analysis suggested that the overall Parkinson’s disease incidence will continue to increase for the next decades. It was projected that China would have 4.787 million Parkinson’s patients by the year 2030, however, the mortality of Parkinson’s disease for both genders in China may keep decreasing.Conclusion: Though the mortality risk may decrease, Parkinson’s disease continues to become more common for both genders in China, especially in the senior-aged population. The burden associated with Parkinson’s disease would continue to grow. Urgent interventions should be implemented to reduce the burden of Parkinson’s disease in China.
Laparoscopic repeat hepatectomy versus conventional open repeat hepatectomy for recurrent hepatocellular carcinoma: A systematic review and meta-analysis
Authors: Hao Fulong , Li Hancong , Li Nan , Li Jiaxin , Wu Hong 
Published: September 2022

BackgroundRepeat hepatectomy has been proven to be an effective treatment in patients with recurrent hepatocellular carcinoma (RHCC). However, for RHCC, it is still controversial whether laparoscopic hepatectomy is superior to conventional ones. The present meta-analysis was carried out to investigate the safety and overall effect of laparoscopic repeat hepatectomy (LRH) to open repeat hepatectomy (ORH) for patients with RHCC.MethodsA meta-analysis was registered at PROSPERO, and the registration number is CRD42021257569. PubMed, Web of Science, and EMBASE were searched based on a defined search strategy to identify eligible studies before 25 April 2022. Data on operative times, bleeding volume, overall complications, 90-day mortality, blood transfusion, length of stay, overall survival rate, and long-term recurrence-free survival rate were subjected to meta-analysis.ResultsOverall, we identified nine studies of LRH versus ORH enrolling a total of 945 patients (460 and 485 underwent LRH and ORH, respectively). The present meta-analysis revealed non-significant differences in operative time, blood transfusion, overall complications, 90-day mortality, 3-year overall survival rate, 5-year overall survival rate, and long-term recurrence-free survival rate between the two groups. Alternatively, comparing LRH with ORH, LRH has less bleeding volume (p &lt; 0.001) and a shorter length of stay (p = 0.005).ConclusionLRH is a feasible and effective treatment strategy for RHCC.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/#searchadvanced, identifier CRD42021257569.