bluuebunny/crossref_metadata_2025_split huggingface dataset:
Management and Outcomes of Pediatric, Adolescent and Young Adult Sarcoma Patients: Results from the French Nationwide Database NETSARC
Authors: Kubicek Pierre , Cesne Axel Le , Lervat Cyril , Toulmonde Maud , Chevreau Christine , Duffaud Florence , Nail Louis-Romée Le , Morelle Magali , Gaspar Nathalie , Vérité Cécile , Castex Marie-Pierre , Penel Nicolas , Saada Esma , Causeret Sylvain , Bertucci François , Perrin Christophe , Bompas Emmanuelle , Orbach Daniel , Laurence Valérie , Piperno-Neumann Sophie , Anract Philippe , Rios Maria , Gentet Jean-Claude , Mascard Éric , Pannier Stéphanie , Blouin Pascale , Carrère Sébastien , Chaigneau Loïc , Soibinet-Oudot Pauline , Corradini Nadège , Boudou-Rouquette Pascaline , Ruzic Jean-Christophe , Lebrun-Ly Valérie , Dubray-Longeras Pascale , Varatharajah Sharmini , Lebbe Céleste , Ropars Mickaël , Kurtz Jean-Emmanuel , Guillemet Cécile , Lotz Jean-Pierre , Berchoud Juliane , Cherrier Grégory , Ducimetière Françoise , Chemin Claire , Italiano Antoine , Honoré Charles , Desandes Emmanuel , Blay Jean-Yves , Gouin François , Marec-Bérard Perrine 
Published: June 2022

Abstract
Background. The initial management of patients with sarcoma is critical. We used the French National Cancer Institute-funded nationwide sarcoma database NETSARC to report the management and oncologic outcomes in children, adolescents and young adults (AYAs) patients with sarcoma at the national level. Patients and methods. Data from patients with sarcoma are collected, regularly monitored and updated using NETSARC. NETSARC was searched for pediatric (<15 years) and AYA (15-30 years) patients with sarcoma, diagnosed from 2010 to 2017. We report the management, locoregional recurrence-free survival (LRFS), progression-free survival (PFS), and overall survival (OS) in children and AYA treated in French reference sarcoma centers (RSC) and outside RSC (non-RSC), and conducted multivariate analyses adjusted for classical prognostic factors to explore survival.Results. We collected data from 3,972 pediatric and AYA patients with sarcoma diagnosed between 2010 and 2017, including 714 children, 1,290 AYAs treated in RSC, and 937 AYAs in non-RSC. Significant differences in compliance to guidelines were observed regarding pre-treatment biopsy (Children: 83.5%; AYA in RSC: 85.9%; AYA in non-RSC 48.1%), pre-treatment imaging (Children: 81.2%; AYA in RSC: 86.8%; AYA in non-RSC: 56.5%) and R0 margins (Children: 52%; AYA in RSC 57.6%; AYA in non-RSC: 20.2%) (p<0.001). 3y-OS rates were 81.6% (95%CI 77.9-84.7) in children, 81.1% (95%CI 78.3-83.6) in AYA in RSC and 82.7% (95%CI 79.4-85.5) in AYA in non-RSC, respectively. LRFS and PFS were improved in AYAs treated in RSC compared to AYAs treated in non-RSC (Hazard Ratios HR: 0.64 and 0.85 with statistical significance at 1% and 10%, respectively). Multivariate regression analysis identified no significant differences in OS in AYAs treated in RSC and in non-RSC (p=0.272).Conclusions. This study highlights the importance for AYA patients with sarcoma to be managed in national sarcoma reference centers involving multidisciplinary medical teams with paediatric and adult oncologists.
Sport and self-esteem in people living with HIV: a cross-sectional study
Authors: PEREZ Lucia GRANDIERE , ALLAVENA Clotilde , SÉCHER Solène , Sylvain DURAND , GRÉGOIRE Antoine , VANDAMME Yves-Marie , Hitoto Hikombo , LEAUTEZ-NAINVILLE Sophie , Michau Christophe , Billaud Eric 
Published: June 2022

Abstract
Background: In the general population, sport activity is associated with better health and better self-esteem. Among people living with HIV (PLHIV), sport activity could be associated with better self-esteem. The main objective of our study was to assess the association between sport activity and self-esteem among people living with HIV. The secondary objectives were to evaluate the association between sport and fatigue, sport and pain.Methods: We performed a cross-sectional observational study among PLHIV in our region. Each adult seen in routine HIV care was proposed to participate to the study. Participants had to fulfill self-questionnaires about sport activity, self-esteem, strength, and pain.Results: Among the 1160 people included in the study, 47% performed sport activity. The self-esteem score was better in the sporting group compared with the non sporting group (Rosenberg mean scale 32.7/40 versus 31.9 ; p=0.01). The FACIT-F total score showed a better strength in the sporting group than in the non sporting group (125 vs 118 p<0.0001). The sporting group had a lower mean pain score (1,1) than the non sporting group (1.4; p=0,004). Conclusions: Among PLHIV in our region, sport activity was associated with better self-esteem, better strength and lower pain. Sport activity should be included in patient care for people living with HIV.
Improving the representativeness of the Tribal Behavioral Risk Factor Surveillance System through data integration
Authors: Chen Sixia , Campbell Janis , Spain Erin , Milligan Alexandra , Snider Cuyler 
Published: June 2022

Abstract
Background. Previous literature showed significant health disparities between Native American population and other populations such as Non-Hispanic White. Most existing studies for Native American Health were based on non-probability samples which suffer with selection bias. In this paper, we are the first to evaluate the effectiveness of data integration methods, including calibration and sequential mass imputation, to improve the representativeness of the Tribal Behavioral Risk Factor Surveillance System (TBRFSS) in terms of reducing the biases of the raw estimates. Methods. We evaluated the benefits of our proposed data integration methods, including calibration and sequential mass imputation, by using the 2019 TBRFSS and the 2018 and 2019 Behavioral Risk Factor Surveillance System (BRFSS). We combined the data from the 2018 and 2019 BRFSS by composite weighting. Demographic variables and general health variables were used as predictors for data integration. The following health-related variables were used for evaluation in terms of biases: Smoking status, Arthritis status, Cardiovascular Disease status, Chronic Obstructive Pulmonary Disease status, Asthma status, Cancer status, Stroke status, Diabetes status, and Health Coverage status. Results. For most health-related variables, data integration methods showed smaller biases compared with unadjusted TBRFSS estimates. After calibration, the demographic and general health variables benchmarked with those for the BRFSS. Conclusions. Data integration procedures, including calibration and sequential mass imputation methods, hold promise for improving the representativeness of the TBRFSS.
Direct and Easily Prepared Nanocomposite Impurity-Free Hydroxyapatite from Locally Delivered CKD as a Potent Catalyst for trans-2-Butene Production
Authors: Nasr Mahmoud , Halawy Samih A. , Abdelkader Adel 
Published: June 2022

Abstract
This project is considered the first successful attempt to directly prepare highly pure nanocomposite hydroxyapatite (HAPT) from cement kiln dust. It is prepared by applying a cost-effective preparation method. This is confirmed by XRD, FT-IR and EDX analyses. The crystallite size of impurity-free hydroxyapatite sample prepared at 500°C was equal to 23.6 nm and showed a hierarchical mesoporous unit, in the nano-scale, with Ca-deficient structure according to SEM-EDX analyses. HAPT exhibits a high population of different acidic sites, i.e. weak, moderate and strong acidic sites, as determined by TG and DSC-TPD experiments using tetrahydrofuran (THF) as a probe molecule. This wide range of acidic sites over HAPT is clearly and positively enhanced its catalytic activity during the conversion of sec-butanol to trans-2-butene. The sec-butanol was converted by 40% into trans-2-butene at 200°C, and gradually increased up to 91.4% conversion at 300°C with % selectivity < 99%. Moreover, our prepared HAPT showed a higher catalytic activity using air as a carrier, during the conversion of sec-butanol, instead of N2-gas as a carrier. A comparison between the catalytic activity of HAPT prepared from the waste CKD and another sample prepared using pure Ca(NO3)2, both calcined at 500°C for 3 h in oxygen, was done during the conversion of sec-butanol in the temperature range of 200-300°C. HAPT from CKD gave unrivaled results compared to the other sample prepared from calcium nitrate. We ascribed this distinguish behavior to both the acidic sites distribution over each sample and their crystallite sizes.
Analysis of Amino Acid Changes in Fusion protein of Virulent Newcastle Disease Virus from vaccinated poultry in Nigerian Isolates
Authors: Funsho-Sanni Olubukola Olubukola , Ella Elijah Ekah , Rogo Dahiru Lawal , Sanni Olufunsho Samuel , Inabo Helen I. , Luka Sodangi Abdulkarim , Shittu Ismaila 
Published: June 2022

Abstract
The roles of Fusion gene in the virulence of Newcastle disease virus has been well established but the extent of its variation among the newly identified XIV, XVII and XVIII genotypes which are predominantly found in Central and West Africa have until recently been understudied and LaSota vaccine strain protection against mortality and morbidity against XIV genotype is the least reported. In this study, F gene of vNDV isolated from samples collected in Nigeria from chicken cadaver from vaccinated chicken flocks showing high mortality, clinical signs and post-mortem lesions attributable to ND during March and April, 2020 were sequenced and analysed for information about genetic changes. Results showed that all isolates from our study have virulent cleavage site sequence 112-RRRKR-116/F117 and clustered within genotype XIVb. Sequence analysis show K78R mutation in the A2 antigenic epitope in all isolates, and more along the F gene which vary in some instances within the isolates. Mutation in this A2 antigenic epitope has been reported to induce escape mutation to monoclonal antibodies generated using NDV LaSota strain. Averages of antigenic variability and percentage homology between the isolates and commonly used vaccines is 0.24; 81.40% and 0.22; 81.90% for LaSota and Komorov vaccines respectively. Nucleotide sequences was assigned accession numbers OK491971-OK491977. Many substitutions were observed in the isolates and some of their functions are yet to be determined.
Additional Late-onset Ocular Hypotensive Effect of Ripasudil on Primary Open-angle Glaucoma
Authors: Sano Kei , Terauchi Ryo , Fukai Kota , Ogawa Shumpei , Noro Takahiko , Tatemichi Masayuki , Nakano Tadashi 
Published: June 2022

Abstract
Purpose: Ripasudil has an additional late-onset ocular hypotensive effect after long-term administration. We evaluated this additional effect of ripasudil and associated factors in primary open-angle glaucoma (POAG).Patients and Methods: We retrospectively reviewed the clinical data of patients with POAG who newly started ripasudil medication without drug change, ocular surgery, or laser treatment. These patients were assigned to two groups: positive group with the late-onset effect and negative group without it. Eyes with a gradual decrease in intraocular pressure (IOP) after starting ripasudil were defined as late-onset positive. Multivariable logistic regression was performed to explore associated factors of this effect.Results: We enrolled 74 eyes of 74 patients with POAG (age, 67.5 ± 10.9 years; mean deviation, -11.2 ± 5.9 dB) and followed them for 14.2 ± 5.0 months. 12 (16.2%) eyes were assigned to the positive group. Retinal nerve fibre layer (RNFL) thickness (73.4 ± 12.9 vs. 64.0 ± 9.8 μm, P = 0.04) and primary IOP (18.8 ± 4.1 vs. 15.8 ± 4.3 mmHg, P = 0.01) before starting ripasudil were higher in the positive group than in the negative group. Multivariable analyses revealed primary IOP (odds ratio [OR], 1.22; 95% confidence interval [CI], 1.01-1.48) and RNFL thickness (OR, 2.76; 95% CI, 1.15-6.63) were significantly associated with late-onset IOP decline.Conclusions: Few patients with advanced POAG showed the late-onset IOP-lowering effect of ripasudil. Multivariable analyses suggested that patients with thicker RNFLs or higher IOPs were more likely to benefit from the additional efficacy of ripasudil.
Analysis of yam starch and its application in synthesis ofNiO nanoparticles using simple green method
Authors: Egu Samuel Attah , Maji D. C. , Idih O. S. , Omale A. 
Published: June 2022

Abstract
Herein is reported green synthesis of starch reduced nickel oxide nanoparticles (SNiONPs). The synthesis involved the use of nickel chloride hexahydrate as nickel precursor, starch as reducing and capping agents and water as solvent. By differing the reaction time, the evolution of growth of the SNiONPs was investigated. The starch and the nanoparticles were characterized using UV-Vis absorption spectroscopy, FTIR, XRD, SEM, XRF, EDS and the starch was studied for proximate composition. The absorption maxima of the as-synthesized materials at different reaction times showed characteristic Ni LSPR peaks. The capping of the nanoparticles was verified using FTIR, XRF and EDS analysis, the particle size was determined using Scherrer equation. SEM image of the starch showed oval to egg-shaped particles while SEM of the nanoparticles showed dispersed oval shape material. The sensitivity of the SNiONPs indicated possible sensitivity in a linear response over concentration range of 1– 6 M H2O2.
Oxyresveratrol attenuates LPS-induced oxidative stress and neuroinflammation through inhibiting PI3K/Akt/NF-κB signaling pathway in mice
Authors: He Yifei , WANG SHANSHAN , Dong Changzhi , Pan Chunxing , Chang Xia , Lin Li , Du Zhiyun 
Published: June 2022

Abstract
Background: Oxyresveratrol (OXY), a polyphenolic compound derived from several plants, has been reported to have anti-oxidative and anti-inflammatory activities. However, the effect and mechanism of OXY in regulating neuroinflammation in vivo is still unclear. Thus, this research focused on the anti-neuroinflammatory effects and mechanism in mice.Methods: A BV-2 cell line and C57BL/6 mice were used to establish LPS-induced neuroinflammation models in vitro and in vivo, respectively. C57BL/6 mice were exposed to LPS (250 μg/kg), OXY (50, 100 mg/kg), resveratrol (100 mg/kg), TPP488 (5 mg/kg). After modeling, behavioral test was established and the molecular mechanism was further analyzed.Results: The pathways enrichment analysis results of the 69 common targets show that the PI3K-Akt pathway and the NF-κB pathway may be the related mechanisms of oxyresveratrol in regulating neuroinflammation. In vitro, OXY at a concentration of 25 μM can significantly inhibit the expression of NO induced by LPS. In vivo, OXY significantly improved cognition impairment and alleviated the neuronal apoptosis caused by LPS. The protective effect of OXY on neuroinflammation is mainly through inhibiting the phosphorylation process of the PI3K/Akt/NF-κB pathway to downregulate the expression of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, Inducible Nitric Oxide Synthase (iNOS), Cyclooxygenase-2 (COX2), Matrix metalloproteinase 9 (MMP9). The oxidative stress and the excessive activation of microglia and astrocytes was suppressed in the brain to alleviate neuroinflammation.Discussion: The research first revealed that OXY can alleviate LPS-induced oxidative stress and neuroinflammation in mice by regulating PI3K/Akt/NF-κB signaling pathway.
The development of speech perception in cochlear implant patients after initial early switch-on
Authors: Kwak Min Young , Kim Yehree , Lee Jee Yeon , Seo Ji Won , Park Hong Ju 
Published: June 2022

Abstract
Objectives Recent studies have demonstrated that early activation of a cochlear implant (CI) has been reported safe. According to their switch-on timing, we analyzed speech perception abilities in prelingual deaf children receiving a sequential bilateral CI and postlingual deaf adults. Design and Setting A retrospective observational study in tertiary medical center Main outcome measures We compared monosyllabic word recognition scores (WRSs) and sentence recognition scores between twenty-eight CI patients who underwent an early switch-on (mean three days after CI) and 70 CI patients having an initial activation one month after CI.Results The WRSs increased rapidly in postlingual deaf adults (31%, 65%, and 78% at one week, one month, and three months after CI) and prelingual deaf children (29%, 52% and 75% at one week, one month, and three months after CI) following the early switch-on. The deaf adults and children in the conventional group reached 28% and 13%, respectively, at one month after CI. Conclusion The early switch-on group showed significantly earlier speech acquisition than the conventional group up to 3 months after CI. Early activation of a cochlear implant (CI) provides the earlier achievement of speech recognition, and it can reduce waiting times for hearing outcomes following CI surgery.
Transcriptomic investigation highlights tumor suppressive effects of TAGAP in lung adenocarcinoma
Authors: Xu Zhanyu , Zheng Zhiwen , Jiang Wei , Huang Liuliu , Deng Kun , Yuan Liqiang , Zheng Tiaozhan , Qin Fanglu , Sun Yu , Qin Junqi , Li Shikang 
Published: June 2022

Abstract
Background: The role of the T cell-activated Rho GTPase activating protein (TAGAP) in immune diseases continues to be explored but has not been yet elucidated for lung adenocarcinoma (LUAD).Methods: The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression dataset (GTEx) databases were used to analyze the difference of TAGAP expression and its correlation with immune cell infiltration, immune checkpoints, and tumor cell stemness scores in pan-cancer and LUAD cohorts. Gene expression single-cell sequencing data from Gene Expression Omnibus (GEO) were used to analyze TAGAP distribution in immune cells. The correlation between TAGAP expression and immune cell markers was validated in 26 pairs of LUAD tissues by Reverse transcription-quantitative PCR (RT-qPCR) and immunohistochemistry. Results: TAGAP was differentially expressed in 27 different cancers tissues and the corresponding adjacent tissues. Its expression level was significantly correlated with immune cell infiltration, immune checkpoints, mDNAsi, and mRNAsi. Moreover, TAGAP was significantly low in LUAD and could be associated with poor prognosis, thereby it can be used as an independent prognostic factor for LUAD. Besides, TAGAP showed a significant positive correlation with B cells, CD4+ T cells, CD8+ T cells, macrophages, neutrophils, and dendritic cells in LUAD. The experimental verification with RT-qPCR and immunohistochemistry showed that TAGAP was significantly positively correlated with CD8 and CD4 expressions. TAGAP expression is linked to a greater response to immune checkpoint blockade treatment in NSCLC. Conclusion: Overall, TAGAP can be a potential LUAD biomarker and might play anti-tumor effects by enhancing the tumor immune cell infiltration in LUAD’s microenvironment, TAGAP might be used as an immunotherapy target to regulate the anti-tumor immune response.
Biosynthesis of nano-inorganic materials by urease and phosphatase positive probiotic bacteria
Authors: Nouri Sabere , Roghanian Rasoul , Emtiazi Giti , Shafiei Rasoul 
Published: June 2022

Abstract
In recent years’ green synthesis has received more attention than chemical synthesis due to its eco-friendly and compatibility. In this study Bacillus subtilis and Bacillus coagulans, two potential probiotics that produce antimicrobial agents were used to synthesize nano-hydroxyapatite and nano-calcite. Grown Bacillus in medium culture containing insoluble calcium phosphate produced nano-hydroxyapatite and nano-crystalline calcite. The nano-hydroxyapatite was purified from nano-calcite by heat treatment and washing with a 200 nm filter. The structures, characteristics, and elemental analysis of nano-sized material were studied by Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, Scanning Electron Microscope, Ultraviolet-visible spectroscopy, Energy dispersive X-Ray, and X-Ray Fluorescence. The results showed that hydroxyapatite is made only in a medium containing insoluble calcium phosphate sublimated with urea which is induced phosphatase and urease. Here, for the first time, the braided bacterial nano-hydroxyapatite similar to the bone structure was made in the medium which induced the production of urease and phosphatase (Maximum 99 U/L) enzymes, and the particle size was less than 100 nm. The ratio of calcium to phosphate in crude hydroxyapatite and calcite crystal particles made by Bacillus coagulans was 2.9, however, this ratio for pure hydroxyapatite was 1.7. Since the particles are made by antibacterial potential probiotics, the biological production of these particles makes them a suitable candidate to be used in toothpaste, bone regeneration, and sanitation products.
Bioinformatics prediction and analysis of co-altered genes as prognostic targets for papillary thyroid carcinoma
Authors: UNAL ULKU , Göv Esra 
Published: June 2022

Abstract
Papillary thyroid carcinoma (PTC) is the most common subtype in thyroid cancers. In this study, altered co-expression analysis was performed to identify molecular signatures that could take a role in disease pathogenesis via comparative analysis of correlation of gene expression between different states (i.e: PTC and non-tumor tissues). As a result, putative systems biomarkers in PTC were determined. Moreover, resultant genes were found to have significantly high performance in distinguishing tumor and non-tumor samples and were identified as prognostic genes. Especially, several molecular signatures such as EPHA3, LYVE1, and TACR2 as receptors, TFCP2L1, MEOX2, and ZFPM2 as transcription factors (TFs), AKR1C2, AOX1, GALNT7 QPCT, GALE, and RPS6KA5 as enzymes came into prominence. The presented genes here will be a crucial resource for new prognostic and therapeutic strategies for PTC.