bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical impact of cardiac magnetic resonance in patients with suspected coronary artery disease associated with chronic kidney disease (AQUAMARINE-CKD study): study protocol for a randomized controlled trial
Authors: Noguchi Teruo , Ota Hideki , Matsumoto Naoya , Morita Yoshiaki , Oshita Akira , Kawasaki Eiji , Kawasaki Tomohiro , Moriwaki Kensuke , Kato Shingo , Fukui Kazuki , Hoshi Tomoya , Watabe Hiroaki , Kanaya Tomoaki , Asaumi Yasuhide , Kataoka Yu , Otsuka Fumiyuki , Takagi Kensuke , Yoneda Shuichi , Sawada Kenichiro , Iwai Takamasa , Matama Hideo , Honda Satoshi , Fujino Masashi , Miura Hiroyuki , Nishimura Kunihiro , Takase Kei 
Published: September 2022

Abstract
Background
Although screening for coronary artery disease (CAD) using computed tomography coronary angiography in patients with stable chest pain has been reported to be beneficial, patients with chronic kidney disease (CKD) might have limited benefit due to complications of contrast agent nephropathy and decreased diagnostic accuracy as a result of coronary artery calcifications. Cardiac magnetic resonance (CMR) has emerged as a novel imaging modality for detecting coronary stenosis and high-risk coronary plaques without contrast media that is not affected by coronary artery calcification. However, clinical use of this technology has not been robustly evaluated.
Methods
AQUAMARINE-CKD is an open parallel-group prospective multicenter randomized controlled trial of 524 patients with CKD at high risk for CAD estimated based on risk factor categories for a Japanese urban population (Suita score) recruited from 6 institutions. Participants will be randomized 1:1 to receive CMR examination that includes non-contrast T1-weighted imaging and coronary magnetic angiography (CMR group) or standard examinations that include stress myocardial scintigraphy (control group). Randomization will be conducted using a web-based system to ensure allocation concealment and incorporate minimization. The primary outcome is a composite of death from CAD, all-cause death, nonfatal myocardial infarction, nonfatal ischemic stroke, ischemia-driven unplanned coronary intervention (percutaneous coronary intervention, coronary bypass surgery, or hospitalization for unstable angina pectoris).
Discussion
This is the first study to look at the implementation of CMR in an outcome-focused patient care pathway. This study will have major implications for the management of patients with CKD at high risk for CAD.
Trial registration:
Japan Registry of Clinical Trials (jRCT) number 1052210075. Registered on September 10, 2021, http://apps.who.int/trialsearch/ListBy.aspx?TypeListing=0
Type of adjuvant endocrine therapy and disease-free survival in patients with early HR-positive/HER2-positive BC: analysis from the phase III randomized ShortHER trial
Authors: DIECI MARIA VITTORIA , BISAGNI GIANCARLO , BARTOLINI STEFANIA , FRASSOLDATI ANTONIO , VICINI ROBERTO , BALDUZZI SARA , D’AMICO ROBERTO , CONTE PIERFRANCO , GUARNERI VALENTINA 
Published: September 2022

Abstract
Background
We evaluated the impact of the type of endocrine therapy on disease-free survival (DFS) in patients with HR-positive/HER2-positive BC enrolled in the phase III ShortHER trial.
Methods
Short-HER randomized 1254 patients with HER2-positive early BC to 9 weeks vs 1 year of adjuvant trastuzumab combined with anthracycline-taxane chemotherapy. The type of adjuvant endocrine was collected during the first 5 years of follow-up and was classified as: aromatase inhibitor (AI), tamoxifen and aromatase inhibitor (TAM-AI), or tamoxifen (TAM). The use of gonadotropin-releasing hormone analogues (GnRHa) was also collected. DFS was calculated from randomization to disease recurrence, second primary tumor, or death.
Results
784 patients with HR-positive BC were included: 60.5% postmenopausal, median age 55 years. The pattern of endocrine therapy was: 59.6% AI, 23.8% TAM, 16.6% TAM-AI. At a median follow up of 8.7 years, patients who received AI had a significantly better DFS vs patients who received TAM or TAM-AI: 8-yr DFS 86.4% vs 79.7%, log-rank P = 0.013 (HR 1.52, 95%CI 1.09–2.11). In multivariate analysis, the type of endocrine therapy maintained a significant association with DFS (HR 1.64, 95% CI 1.07–2.52, p = 0.025 for TAM/TAM-AI vs AI). Among premenopausal patients aged ≤ 45 years (97% receiving TAM or TAM-AI), the use of GnRHa was associated with longer DFS: 8-yr DFS rate 85.2% vs 62.6% (log-rank p = 0.019, HR 0.41, 95% CI 0.19–0.88).
Conclusions
In this post-hoc analysis of the ShortHER trial adjuvant treatment with AI was independently associated with improved DFS. Subgroup analysis in young premenopausal patients suggests benefit with ovarian suppression.
Trial registration:
NCI ClinicalTrials.gov number: NCT00629278. Registered 5 March 2008. Retrospectively registered (first patient in December 2007).
Pharmacological investigation on acute and sub-acute studies of Arsenicum album in Experimental Rats
Authors: Lal Roshan , Sharma Mahima , Behera Sangita , Regar Raj Kumar , Tripathi Deepika , Kumar GV Narasimha , Singh Satvinder , Verma Digvijay , Gupta Pankaj , Kaushik Subhash , Khurana Anil 
Published: September 2022

Abstract
Purpose
Arsenic album in its various potencies are frequently prescribed by homoeopathic practitioners for wide range of human anomaly in everyday life. However, reports on safety and effects of Arsenic album are not available till date to support its usage. Therefore the objective of study is to evaluate the acute and subacute oral toxicity of Arsenic albumin 6C, 30C and 200C in experimental rats.
Materials and Methods
Arsenic album (6C, 30C, 200C) was administered orally at 2000 µl/kg to access acute toxicity in Wistar rats and observed for toxic signs up to 14 days. For subacute oral toxicity study, it was administered for 28 days. Animals were observed for clinical signs, change in body weights, feed intake and water intake. Hematological, biochemical, organ weight, histopathological analysis were assessed.
Results
No mortality at a dose of 2000 µl/kg of Arsenic album in acute toxicity study, which indicates that oral LD50 of arsenic album (6C, 30C, 200C) is > 2000 µl/kg. In subacute toxicity study, Arsenic album (6C, 30C, 200C) orally at 200 µl/kg did not show any significant changes in body weight, feed consumption, water intake, hematological and biochemical parameters compared to normal group. Furthermore, no pathological changes were observed in histopathology of treated rats compared to normal group.
Conclusion
Collectively, results suggest that the Arsenic album (6C, 30C, 200C) is safe and produces no toxicity when administered for prolonged duration at 200 µl/kg in Wistar albino rats.
Spinocerebellar Ataxia type 3 is rare in the Peruvian Population
Authors: Cornejo-Olivas Mario , Solis-Ponce Lesly , Araujo-Aliaga Ismael , Milla-Neyra Karina , Ortega Olimpio , Illanes-Manrique Maryenela , Mazzetti Pilar , Manrique-Enciso Carla , Cubas-Montecino Diana , Saraiva-Pereira Maria Luiza , Jardim Laura B. , Sarapura-Castro Elison 
Published: September 2022

Abstract
Spinocerebellar ataxia type 3 or Machado-Joseph Disease (MJD/SCA3) is the most prevalent autosomal dominant cerebellar ataxia worldwide, but its frequency varies by geographic region. We describe MJD/SCA3 patients diagnosed in a tertiary healthcare institution in Peru. In a cohort of 341 individuals with clinical ataxia diagnosis, seven MJD/SCA3 probands were identified and their pedigrees extended, detecting a total of 18(5%) MJD/SCA3 cases. Out of 682 alleles from this cohort, the 23-CAG repeat was the most common ATXN3allele (32.1%), followed by the 14-CAG repeat allele (26%). Normal alleles ranged from 12 to 38 repeats while pathogenic alleles ranged from 61-75 repeats. We identified 101 large normal (LN) alleles (14.8%). Five out of seven families declared an affected family member traced back to foreign countries (England, Japan, China, and Trinidad and Tobago). MJD/SCA3 patients showed ataxia, accompanied by pyramidal signs, dysarthria and dysphagia as well as abnormal oculomotor movements. In conclusion, ATXN3 allelic distribution in non-MJD/SCA3 patients with ataxia is similar to the distribution in normal individuals around the world, whereas LN allele frequency reinforces no correlation with the frequency of MJD/SCA3. Evidence of an abnormal MJD/SCA3 phenotype was not found. Further haplotypes are required to confirm the foreign origin of MJD/SCA3 in the Peruvian population.
Clinicopathological features and prognostic factors analysis of multiple synchronous distinct subtypes of primary thyroid carcinoma
Authors: Dai Quanwei , Fu Xinghao , Ding Yalei , He Qi , Qiu Xinguang 
Published: September 2022

Abstract
Purpose: To investigate the clinicopathological features, diagnosis, treatment and prognosis of multiple synchronous distinct subtypes of primary thyroid carcinomas
Methods: The clinical data of 68 cases of synchronous carcinomas of the thyroid(STC) admitted to the Department of Thyroid Surgery in our hospital from January 2013 to December 2021 were reviewed.，According to the pathological type, they were divided into the Synchronous differentiated and differentiated thyroid Carcinoma(SDDTC)group (42 cases), the Synchronous medullary and differentiated thyroid Carcinoma(SMDTC)group (18 cases), and the Synchronous Anaplastic and differentiated thyroid Carcinoma (SADTC)group (8 cases). The diagnosis, treatment and survival of patients in each group were analyzed.
Results: Women with coexisting thyroid cancer were predominant (59 cases). Most of the symptoms were found on physical examination and neck mass. In the SDDTC group, the median age of patients in the SDDTC group, SADTC group and SMDTC group was 47.5 (28-74) years old, 68.5 (26-75) years old, and 56.5 (39-74) years old. The age of the SADTC group and SMDTC group was older than that of the SDDTC group (P=0.04, P=0.03), and lymph node metastasis was more likely to occur (P=0.02, P<0.01).The disease course time, tumor location, clinical stage, and mortality of the SADTC group were significantly different from those of the SDDTC group and SMDTC survival was 38.5 months. The tumor-free survival was 0-90 months, 19.1% of patients developed distant metastasis, 11.8% of patients had postoperative recurrence, and the survival rate was 91.18%. Cox model multivariate analysis showed that cervical lateral lymph node metastasis and tumor stage III/IV were independent risk factors for progression-free survival(PFS).The comparison results of the survival curves showed that the overall survival(OS)of the patients in the SADTC group was significantly worse (P<0.01), while there was no significant difference in the PFS of different pathological types (χ2=5.024, P=0.081).The OS of different treatment methods was significantly different (P=0.002), but there was no significant difference in OS between local recurrence and distant metastases with or without surgery (χ2=0.954, P=0.329).
Conclusions: The STC has relatively unique clinical characteristics, and most patients can get a better prognosis after radical surgery. Pathological type, lateral cervical lymph node metastasis, tumor stage and treatment are important factors which affect the prognosis of the disease. Since there are two distinct tumors with different aggressiveness, treatment options and prognosis, individualized management is required.
Next-generation network transmission control protocol based on deep learning
Authors: M Nisha Angeline , Shivaranjani M , Manikandan S K , Gokul C 
Published: September 2022

Abstract
Long-term networks suffer from higher performance degradation and larger delays, making 5G wireless communications networks the best option for broadcasting and dealing with emergency situations. Obstructions and misalignment issues in the 5G millimetre wave spectrum can cause the link to be severed and the network to be severely impacted.. An intelligent Transport Control Protocol (iTCP) for a disaster 5G millimetre wave system is proposed in this study.iTCP learns about a node's movement and transmission power, and adjusts the congestion control window based on this information. In terms of network security and performance, iTCP outperforms TCP NewReno, Vegas, Westwood, Cubic, and Compound.
Neural activation patterns evoked by social inference in the laboratory predict the number of real-life social contacts
Authors: Tusche Anita , Spunt Robert , Paul Lynn , Tyszka Julian , Adolphs Ralph 
Published: September 2022

Abstract
We regularly infer other people’s thoughts and feelings from observing their actions, but it remains unknown how this ability contributes to successful social behavior and interactions. We show that neural activation patterns during social inferences obtained in the laboratory predict the number of social contacts in the real world in three community samples (total n=126) and a population with autism (n=23) (social network index, SNI). Cross-validated predictions were localized selectively to the right posterior superior temporal sulcus (pSTS) and were specific for social inferences, not nonsocial ones. Moreover, the multivariate pattern of brain activation during social inferences generalized across participants in the neurotypical and autism group (cross-sample prediction). Brain activations in pSTS also predicted autism-like trait scores (AQ, SRS-2) in neurotypical individuals and symptom severity scores (ADOS SA) in the autism group. These findings indicate that neural activations produced while thinking about other people’s mental states predict variance in other indices of social functioning - beyond the number of real-world social connections. Together, these findings point towards social inference as a latent variable that comes into play relatively ubiquitously in real-life social behavior. The results also demonstrate the potential of using localized, process-specific neurobiomarkers of cognitive abilities that contribute to social behavior as robust predictors of people’s social functioning in the real world.
Predicting pathological highly invasive lung cancer from preoperative 18F-FDG PET/CT with multiple machine learning models
Authors: Onozato Yuki , Iwata Takekazu , Uematsu Yasufumi , Shimizu Daiki , Yamamoto Takayoshi , Matsui Yukiko , Ogawa Kazuyuki , Kuyama Junpei , Sakairi Yuichi , Kawakami Eiryo , Iizasa Toshihiko , Yoshino Ichiro 
Published: September 2022

Abstract
Purpose: The efficacy of sublobar resection of primary lung cancer have been proven in recent years. However, sublobar resection for highly invasive lung cancer increases local recurrence. We developed and validated multiple machine learning models predicting pathological invasiveness of lung cancer based on preoperative 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) and computed tomography (CT) radiomic features.
Methods: Overall, 873 patients who underwent lobectomy or segmentectomy for primary lung cancer were enrolled. Radiomics features were extracted from preoperative PET/CT images with the PyRadiomics package. Seven machine learning models and an ensemble of all models (ENS) were evaluated after 100 iterations. In addition, the probability of highly invasive lung cancer was calculated in a nested cross-validation to assess the calibration plot and clinical usefulness, and to compare to consolidation tumour ratio (CTR) on CT images, one of the generally used diagnostic criteria.
Results: In the training set, when PET and CT features were combined, all models achieved an area under the curve (AUC) of ≥ 0.880. In the test set, ENS showed the highest mean AUC of 0.880 and smallest standard deviation of 0.0165, and when the cutoff was 0.5, accuracy of 0.804, F1 of 0.851, precision of 0.821, and recall of 0.885. In the nested cross-validation, the AUC of 0.882 (95% CI: 0.860-0.905) showed a high discriminative ability, and the calibration plot indicated consistency with a Brier score of 0.131. A decision curve analysis showed that the ENS was valid with a threshold probability ranging from 3% to 98%. Accuracy showed an improvement of more than 8% over the CTR.
Conclusion: The machine learning model based on preoperative 18F-FDG PET/CT images was able to predict pathological highly invasive lung cancer with high discriminative ability and stability. The calibration plot showed good consistency, suggesting its usefulness in quantitative risk assessment.
Utero-vaginal anastomosis in cases of cervical malformations; Long term follow- up and fertility challenges
Authors: Fouad Reham , Zayed Mohamed 
Published: September 2022

Abstract
Purpose: To study the long-term results of utero-vaginal anastomosis in cases of cervical malformations.
Methods: this is a retrospective cohort study. Nine patients presented with cryptomenorrhea due to cervical malformations (5 patients with cervical agenesis and vaginal aplasia, 2 patients with cervical agenesis and upper vaginal aplasia, and two patients with cervical dysgenesis in form of cervical obstruction). Five patients had utero-vaginal anastomosis (UVA) with McIndoe vaginoplasty. Four patients had UVA without vaginoplasty. Follow up was done by transabdominal and/or transvaginal ultrasound monthly for the first three months then every six months thereafter for a duration that ranged from 15 to 82 months.
The main outcome measures are achieving menstruation, dysmenorrhea, pelvic inflammatory disease (PID), needed interventions after primary surgery, infertility, and pregnancy rate.
Results: 9 (100%) patients achieved menstruation, one (12%) experienced severe dysmenorrhea, two (22%) had PID, seven (78%) needed dilatation of the anastomosis site, three (33%) needed reoperation, 9 (100%) had primary infertility, two (28.5%) achieved clinical pregnancy, and only one (14%) ended by live birth.
Conclusion: Conservative surgeries for cervical malformation is a promising choice for relieving the obstructive symptoms. Regular dilatation is recommended. Pregnancy is a remote hope that is hindered by many challenges.
Identification and Validation of RNA Methylation-Related Related Alternative Splicing Gene Signature for Low Grade Glioma to Predict Survival and Immune Landscapes
Authors: Ma Chenggong , Bao Yunong , Xu Jiazhe , Xiao Bo , Li Hui 
Published: September 2022

Abstract
Background
Lower-grade glioma (LGG) is a crucial pathological type of Glioma. The present study aimed to explore multiple RNA methylation regulator-related AS events and investigate their prognostic values in LGG.
Methods
The prognostic model for Low-Grade Glioma was established by using the LASSO regression analysis. To validate prognostic value, we performed Kaplan-Maier survival analysis, ROC curves and nomograms. The ESTIMATE algorithm, the CIBERSORT algorithm and the ssGSEA algorithm were utilized to explore the role of the immune microenvironment in LGG. Subsequently, we then used GO, KEGG and GSEA enrichment analysis to explore the functional roles of these genes. In addition, we employed the GDSC database to screen potential chemotherapeutic agents.
Results
Eight RNA methylation related AS events were involved in construct a survival and prognosis model, which had good ability of independent prediction for patients with LGG. Patients in the high-risk group had shorter life expectancy and higher mortality, while patients in the low-risk group had a better prognosis. We constructed a nomogram which showed an excellent predictive performance for individual OS. The risk score exhibited a close correlation with some immune cells and expression of immune checkpoints. Patients in high-risk group were characterized by immunosuppressive microenvironment and poor response to immunotherapy, and were sensitive to more chemotherapeutic drugs. Pathway and functional enrichment analyses further confirmed that significant differences existed in immune landscape between the two subgroups.
Conclusion
The prognostic RNA Methylation-related Alternative Splicing signature we constructed could constitute a promising prognostic biomarker, which could serve to optimize treatment regimens.
Cholesterol removal in goat milk cheese and its chemical, textural
and sensory evaluation during storage
Authors: bastola Krishna 
Published: September 2022

Abstract
The experiment was conducted in a 2 x 3 x 3 factorial arrangement by having
two types (control and cholesterol reduced) goat milk cheeses, which were assigned to
three different storage temperatures (-18, 4, and 22°C) and three different storage
periods (0, 2, and 4 months). The objectives of this study were to: (1) evaluate
cholesterol removal rate of the CR cheese, (2) determine nutrient composition of CC
and CR cheeses and (3) compare pH, lipolysis, textural and sensory characteristics of
the 2 types of cheeses stored at 3 different temperatures for 3 storage periods. Results
showed that mean contents (%) of moisture, fat, ash, protein, and carbohydrate of CC
cheeses were: 41.4, 26.9, 3.87, 28.2 and 3.87, and those of CR cheeses were: 41.1, 22.9,
4.84, 35.1, and 3.93, respectively. The fat content of CR cheese was significantly lower
than that of CC cheese, indicating that significant part of fat was lost during cream
separation of the original cheese milk. Mean cholesterol concentration (mg/100g
cheese) of control and cholesterol removed cheese were 115.062 and 81.97 respectively,
suggesting that the cholesterol removal rate was only 28.7 %, which is substantially
lower than previous reports on bovine milk cheese studies. With respect to pH changes
in CC and CR cheeses during the 4 month storage at -18, 4 and 22 °C temperatures, CC
cheese had lower pH than CR cheese. There were no significant differences in pH
values for the different temperatures. However, the 2-way interaction effect of cheese
type x storage period showed significant (P<0.01) differences in pHs, Concerning
textural characteristics of the experimental cheeses, the textural parameters of CC
cheese had higher mean values than those of CR cheese for fresh samples. The ADV
values of control cheeses were higher than that of CR cheese samples. The 3-way
viii
interaction effect of cheese type x temperature x storage period showed highly
significant effect on acid degree value in both CC and CR cheeses. The 2-way
interaction of storage time x batch effect was also significant (P<0.01 and P<001) for
ADV values.The results of sensory evaluation on the CC and CR goat Cheddar cheeses
revealed that cooked flavor was not affected by main factors nor by their interactions.
However, storage period made greater influence on sweetness, freshness, high acid,
coarse, crumbly, sandy and color of the experimental cheese samples. On the other hand,
storage temperature had no impact on any of the sensory properties.
Development of a test strip for rapid detection of Gymnodinium catenatum
Authors: Fang Junhua , Li Shuyue , Liu Xiaoxiao , He Weixin , Li Lingyue , Zhang Jiazhao , Zhang Changgong , Zhou Kefu 
Published: September 2022

Abstract
Harmful algal blooms (HABs) are major ecological and environmental problems in China's coastal waters and seriously threaten the stability of the marine ecosystem and human health. Gymnodinium catenatum is a toxic red tide dinoflagellate. It can produce paralytic shellfish toxins (PSP), which cause serious hazards to marine organisms, public health and safety. In this paper, a test strip based on colloidal gold immunochromatography (GICG) was developed for the rapid detection of Gymnodinium catenatum. The experimental results showed that the test trip has good specificity and sensitivity. It not only detects the different components of Gymnodinium catenatum but also may detect algal toxins. The lowest density of Gymnodinium catenatum that can be detected by this test strip is approximately 120 cells/mL. Cross-reaction indicated that the test strip had a high specificity for Gymnodinium catenatum. This test strip provides a rapid method for on-site detection of Gymnodinium catenatum and a reference method for the monitoring of other harmful algae to serve as an early warning of upcoming red tides. It also provides a new way to prepare more detection methods for toxic algal toxins.