bluuebunny/crossref_metadata_2025_split huggingface dataset:
Large-scale crowdsourced radiotherapy segmentations across a variety of cancer anatomic sites: Interobserver expert/non-expert and multi-observer composite tumor and normal tissue delineation annotations from a prospective educational challenge
Authors: Wahid Kareem A. , Lin Diana , Sahin Onur , Cislo Michael , Nelms Benjamin E. , He Renjie , Naser Mohammed A. , Duke Simon , Sherer Michael V. , Christodouleas John P. , Mohamed Abdallah S. R. , Murphy James D. , Fuller Clifton D. , Gillespie Erin F. 
Published: October 2022

AbstractClinician generated segmentation of tumor and healthy tissue regions of interest (ROIs) on medical images is crucial for radiotherapy. However, interobserver segmentation variability has long been considered a significant detriment to the implementation of high-quality and consistent radiotherapy dose delivery. This has prompted the increasing development of automated segmentation approaches. However, extant segmentation datasets typically only provide segmentations generated by a limited number of annotators with varying, and often unspecified, levels of expertise. In this data descriptor, numerous clinician annotators manually generated segmentations for ROIs on computed tomography images across a variety of cancer sites (breast, sarcoma, head and neck, gynecologic, gastrointestinal; one patient per cancer site) for the Contouring Collaborative for Consensus in Radiation Oncology challenge. In total, over 200 annotators (experts and non-experts) contributed using a standardized annotation platform (ProKnow). Subsequently, we converted data into NIfTI format with standardized nomenclature for ease of use. In addition, we generated consensus segmentations for experts and non-experts using the STAPLE method. These standardized, structured, and easily accessible data are a valuable resource for systematically studying variability in segmentation applications.
Influenza Vaccine Effectiveness Against Influenza A(H3N2)-Related Illness in the United States During the 2021–2022 Influenza Season
Authors: Price Ashley M. , Flannery Brendan , Talbot H. Keipp , Grijalva Carlos G. , Wernli Karen J. , Phillips C. Hallie , Monto Arnold S. , Martin Emily T. , Belongia Edward A. , McLean Huong Q. , Gaglani Manjusha , Mutnal Manohar , Geffel Krissy Moehling , Nowalk Mary Patricia , Tartof Sara Y. , Florea Ana , McLean Callie , Kim Sara S. , Patel Manish M. , Chung Jessie R. 
Published: October 2022

AbstractBackgroundIn the United States, influenza activity during the 2021-2022 season was modest and sufficient enough to estimate influenza vaccine effectiveness for the first time since the beginning of the COVID-19 pandemic. We estimated influenza vaccine effectiveness against lab-confirmed outpatient acute illness caused by predominant A(H3N2) viruses.MethodsBetween October 2021 and April 2022, research staff across 7 sites enrolled patients aged ≥6 months seeking outpatient care for acute respiratory illness with cough. Using a test-negative design, we assessed VE against influenza A(H3N2). Due to strong correlation between influenza and SARS-CoV-2 vaccination, participants who tested positive for SARS-CoV-2 were excluded from vaccine effectiveness estimations. Estimates were adjusted for site, age, month of illness, race/ethnicity and general health status.ResultsAmong 6,260 participants, 468 (7%) tested positive for influenza only, including 440 (94%) for A(H3N2). All 206 sequenced A(H3N2) viruses were characterized as belonging to genetic group 3C.2a1b subclade 2a.2, which has antigenic differences from the 2021–2022 season A(H3N2) vaccine component that belongs to clade 3C.2a1b subclade 2a.1. After excluding 1,948 SARS-CoV-2 positive patients, 4,312 patients were included in analyses of influenza VE; 2,463 (57%) were vaccinated against influenza. Effectiveness against A(H3N2) for all ages was 36% (95%CI, 20-49%) overall; 40% (95%CI, 24-53%) for those aged 6 months-49 years; and 10% (95%CI, -60-49%) for those aged ≥50 years.ConclusionInfluenza vaccination in 2021–2022 provided protection against influenza A(H3N2)-related outpatient visits among young persons, with no measurable protection among older adults.
Hormone overdose and misuse in Chinese transgender and gender non-conforming population: A mixed-methods study protocol
Authors: Zeng Ben-tuo , Pan Hui-qing , Li Li-ping , Lan Tian-meng , Ye Zhen-yu , Wang Peng-fei , Liu Yang 
Published: October 2022

AbstractBackgroundThere is no existing research on hormone overdose and misuse (HODM) in Chinese transgender and gender non-conforming (TGNC) population, and little is known in this field.ObjectivesWe aim to determine the definition and criteria of HODM in Chinese TGNCs, address the rate of HODM in Chinese TGNC population, explore related factors and behavioral risks, identify the probable causes, and explore long-term effects.MethodsWe propose: (1) a mixed-method study comprising expert panel meetings and stakeholder engagement to identify HODM criteria, types and grades; (2) a cross-sectional study to quantify HODM prevalence, related factors and behavioral risks; (3) semi-structured interviews and focus groups to explore HODM motivations and reasons; and (4) a prospective cohort study to evaluate HODM long-term effects.EthicsThe study protocol was approved by the Medical Ethics Committee of Xiamen University (XDYX202210K27).DisseminationResults will be published in international peer-reviewed journals, and a public-oriented version of the main findings will be prepared and disseminated through social media and online communities. The study will be completed before September 2023 except for the cohort study. Preliminary findings of the cohort study will be reported by March 2026.
MeCP2 regulates Gdf11, a dosage-sensitive gene critical for neurological function
Authors: Bajikar Sameer S. , Anderson Ashley G. , Zhou Jian , Durham Mark A. , Trostle Alexander J. , Wan Ying-Wooi , Liu Zhandong , Zoghbi Huda Y. 
Published: October 2022

ABSTRACTLoss- and gain-of-function of MeCP2 causes Rett syndrome (RTT) and MECP2 duplication syndrome (MDS), respectively. MeCP2 binds methyl-cytosines to finely tune gene expression in the brain, though identifying genes robustly regulated by MeCP2 has been difficult. By integrating multiple transcriptomics datasets, we identified that MeCP2 finely regulates Growth differentiation factor 11 (Gdf11). Gdf11 is down-regulated in RTT mouse models and is inversely up-regulated in MDS mouse models. Strikingly, genetically normalizing Gdf11 dosage levels improved several behavioral deficits in a mouse model of MDS. Next, we discovered that losing one copy of Gdf11 alone was sufficient to cause multiple neurobehavioral deficits in mice, most notably hyperactivity and decreased learning and memory. This decrease in learning and memory was not due to changes in proliferation or numbers of progenitor cells in the hippocampus. Lastly, loss of one copy of Gdf11 decreased longevity and survival in mice, corroborating its putative role in aging. Our data demonstrate that Gdf11 dosage is important for brain function.
A novel taurine-respiring murine gut bacterium contributes to colonization resistance against enteropathogens
Authors: Ye Huimin , Borusak Sabrina , Eberl Claudia , Hanson Buck T. , Zwirzitz Benjamin , Herbold Craig W. , Pjevac Petra , Hausmann Bela , Stecher Bärbel , Schleheck David , Loy Alexander 
Published: October 2022

AbstractTaurine-respiring gut bacteria produce H2S with ambivalent impact on host health. We report the isolation and genomic-ecophysiological characterization of the first taurine-respiring mouse gut bacterium.Taurinivorans murisrepresents a new widespread species with protective capacity against pathogens and differs from the human gut sulfidogenBilophila wadsworthiain its sulfur metabolism and host distribution. Despite alternative physiologies, taurine respiration was the mainin vivolifestyle ofT. murisindependent of mouse diet and genotype. In gnotobiotic mice,T. murisselectively enhanced the activity of a sulfur metabolism gene-encoding prophage and provided slightly increased colonization resistance againstSalmonellaTyphimurium, which showed reduced expression of galactonate catabolism genes. We identifiedT. murisas the dominant sulfidogen of a mouse microbiota that conferred H2S-mediated protection againstKlebsiella pneumoniaein a previous study. Together, we revealed the realized physiological niche of a key murine gut sulfidogen and its impact on pathogen and phage gene expression.One sentence summaryOur work identified and characterized a new core member of the murine gut microbiota, revealed sulfidogenic taurine respiration as its predominantin vivolifestyle, and emphasizes its protective function in pathogen colonization.
Disentangling positive vs. relaxed selection in animal mitochondrial genomes
Authors: Zwonitzer Kendra D. , Iverson Erik N. K. , Sterling James J. , Weaver Ryan J. , Maclaine Bradley A. , Havird Justin C. 
Published: October 2022

AbstractDisentangling different types of selection is a common goal in molecular evolution. Elevated dN/dS ratios (the ratio of nonsynonymous to synonymous substitution rates) in focal lineages are often interpreted as signs of positive selection. Paradoxically, relaxed purifying selection can also result in elevated dN/dS ratios, but tests to distinguish these two causes are seldomly implemented. Here, we reevaluated seven case studies describing elevated dN/dS ratios in animal mtDNA and their accompanying hypotheses regarding selection. They included flightless vs. flighted lineages in birds, bats, and insects, and physiological adaptations in snakes, two groups of electric fishes, and primates. We found that elevated dN/dS ratios were often not caused by the predicted mechanism, and we sometimes found strong support for the opposite mechanism. We discuss reasons why energetic hypotheses may be confounded by other selective forces acting on mtDNA and caution against overinterpreting molecular “spandrels”, including elevated dN/dS ratios.
Gastric administration of Cis-9, trans-11 and trans-10, cis-12 conjugated linoleic during the pregestational and gestational periods does not influence the follicular endowment of the progeny
Authors: de Freitas Danielle Storino , de Gouvêa Lopes Guilherme Antonio , Nascimento Barbara Rodrigues , Madureira Ana Paula , Campos-Junior Paulo Henrique Almeida 
Published: October 2022

AbstractFetal programming suggests that maternal stimulation and nutrition during the period of fetal development can program the progeny. Conjugated linoleic acid (CLA), an isomer of linoleic acid, has been characterized in several aspects, but few studies have been performed on its involvement in reproduction and fetal programming. The aim of this study was to evaluate the F1, F2 and F3 progeny of female mice supplemented with CLA during the pregestational and gestational periods with respect to biometric and reproductive parameters, as well as ovarian morphophysiology. The F1 progeny of mothers supplemented with CLA exhibited stable weight gain, while the F2 progeny showed no effects (P=0.0187 and P=0.0245, respectively). A reduction in Lee’s Index was observed in both generations at the second post-weaning evaluation week in the animals treated with CLA (P=0.0100 and P=0.0078, respectively). The F2 generation showed an increase in the anogenital index in both sexes of the animals treated with CLA (P= 0.0114 and P<0.0001, female and male respectively). CLA administration to mothers did not affect any of the following in their progeny: ovarian follicle mobilization (P>0.05), follicle number (P>0.05) and the integrated density of the lipid content of oocytes included in antral follicles (P>0.05). This study evaluated the use of CLA in mothers and found that it did not affect the progeny regarding murine reproductive performance, suggesting that this supplement can be used safely.
An estimation of bio-methane and energy project potentials of municipal solid waste using landfill gas emission and cost models
Authors: Usman Abdullahi Mohammed 
Published: October 2022

PurposeThe purpose of the study is to analyse municipal solid waste (MSW) disposed of in Jimeta-Yola metropolis for landfill gas (LFG), methane and project viability potential.Design/methodology/approachThe data was collected daily from landfills for four weeks. About 7,329.55 Mg/year of waste was analysed. These waste were separated into bio-degradable components i.e. paper and textile (263.66 Mg), non-food organic (681.45 Mg), wood and straw (189.50 Mg) and food and kitchen waste (1797.20 Mg). Non-degradable components include plastics, polythene bags, metals, sand, stones, cans etc. (4397.73 Mg). The component's characteristics such as a number of samples, weight, volume, landfill age etc. were measured. The waste, methane (CH4) and energy potential were also analysed using LFG energy cost model.FindingsThe landfills received 15 Gg/year of MSW and emit 0.31 Gg/year of LFG having CH4 content of 82.95 Mg in 2016. These can produce 33.78 GWh of heat energy equivalent to 10.14 GWh of electricity analytically. Therefore, between 2016 and 2022, about 2.24 Gg CH4 and 5201.32 MWh of electricity were wasted. Henceforth, proper management of these waste substances can produce 186.4 Gg CH4 which will generate 432.52 GWh of electricity. The most economically viable project is an electricity project generating 418 kW/year at a sale price of $1.14/kWh (58.38/kWh) and a payback period of 11 years.Practical implicationsRaw LFG collected can be used in heating brick kilns, boilers, furnaces and greenhouses. When treated, the LFG can produce renewable natural gas (RNG), which is used in energy generation and various domestic, vehicle and industrial applications.Social implicationsThe analytical energy generation can provide gross revenue of ₦19.46bn at an average of ₦192.71million/year. Using Landfill Gas Emissions Model (LandGEM) model, the gross and net revenue will be $0.42m and $0.28m yearly, respectively. The project can provide jobs and economic boost to the immediate community through associated ripple effect.Originality/valueThe research is a pre-feasibility study for LFG to gas or electricity projects in Jimeta-Yola. The study contributed to the body of knowledge as a source of literature for further studies locally and globally.
Tangible and intangible hotel in-room amenities in shaping customer experience and the consequences in the with-corona era
Authors: Kim Jinkyung Jenny , Lee Jin-Soo , Han Heesup 
Published: October 2022

Purpose
This study aims to draw on customer experience theory to shed light on how hotel in-room amenities foster customer experience, which continues to form brand attitude and loyalty before and during the pandemic. Also, this study assesses the impact of the pandemic in the relationships among proposed constructs on the basis of risk perception theory.

Design/methodology/approach
A quantitative approach was deployed using a total of 379 responses, for evaluating the measurement model through confirmatory factor analysis and testing proposed hypotheses through structural equation modeling.

Findings
The findings provide initial support for the predictions, except for the influence of brand attitude on brand loyalty before the pandemic. Particularly, the analysis results observe that the effect of tangible amenities on customer experience was stronger before the COVID-19, whereas the impact of intangible amenities on customer experience is greater during the pandemic. Furthermore, the results validate the significant moderating influence of the COVID-19 pandemic in the path between customer experience and brand loyalty.

Practical implications
This present study guides hotel professionals to be more effective in the management of appropriate in-room amenity to create a satisfactory customer experience, which contributes to brand loyalty in the with-corona era.

Originality/value
The study differs from earlier studies in that it investigates how the pandemic changes the role of hotel in-room amenities on customer experience, which, in turn, increases brand attitude and brand loyalty for the first time.
Stigma, discrimination and HIV or AIDS: an empirical investigation of Asian immigrants and refugees in Canada
Authors: Ullah Akm Ahsan , Huque Ahmed Shafiqul 
Published: October 2022

Purpose
HIV or AIDS remains invisible and dismissed by most South Asians living in Canada as HIV or AIDS issues are perceived as an offshoot of Western lifestyle linked with drug use and promiscuity. This paper aims to look into how people living with HIV or AIDS (PLWHA) cope with prejudice and stigma.

Design/methodology/approach
To guide this research, a constructivist grounded theory approach was adopted as the theoretical and methodological framework. The authors reached the participants through a Toronto-based group that works with PLWHA. The authors chose their respondents in a snowball method and interviewed them both in person and online.

Findings
This paper identifies how South Asian immigrants and refugees/refugees with HIV or AIDS claimants are vulnerable to discrimination in Canada due to the following factors, which include but are not limited to: a lack of information about HIV and AIDS incidence in the community; and the Canadian health system's inability to respond appropriately to the lack of information.

Practical implications
HIV service engagements should take place within the context of a constellation of local traditions, or standardized expectations of patient engagement with HIV services can be counterproductive.

Originality/value
It is critical that governmental action prioritizes increasing public understanding of stigma. To minimize the consequences of HIV-related discrimination and stigma, misconceptions about HIV transmission must be debunked.
Tracing the lean thinking in supply chain management: a comprehensive main path analysis
Authors: Yu Dejian , Ye Tong 
Published: October 2022

Purpose
The purpose of this paper is to contribute to the existing knowledge system of lean supply chain management (LSCM), i.e. by using main path analysis, and the authors extract the main development track of LSCM. It is advantageous for relevant scholars to deepen their understanding of this academic field from a bibliometrics view to grasp the future directions better.

Design/methodology/approach
Structuring a citation network with the processed data set containing 866 papers and relevant information collected from Web of Science (WoS). Conducting review analyses aiming at the main paths that are extracted from the above citation network.

Findings
There are two different evolution paths in LSCM field, i.e. improving corporate sustainability performance through combining lean and green practices, and seeking the balance between lean and agility to structure leagile supply chain for specific industries. LSCM research studies mainly focus on five aspects: (1) establishment and development of LSCM theory; (2) structuration of lean supply chain; (3) research studies of the relationship between LSCM and corporate performance; (4) supply chain evaluation system; and (5) review and vista of LSCM field. The intersection of two knowledge evolute routes would be Industry 4.0, which is an integrated theory system combining lean, agility, green and other supply chain thinking.

Research limitations/implications
The data set collected from WoS cannot contain all the research studies about LSCM is the main research limitation. Sustainability, as represented by environmental performance, will continue to be a major pursuit of this field. Integrating LSCM in Industry 4.0 will be the next hotspot in LSCM field.

Practical implications
Providing the main research contents and common methods of LSCM field. It is conducive to deepening the understanding of relevant practitioners and scholars to LSCM field from a dynamic perspective.

Originality/value
To the best of the authors’ knowledge, it is the first time to reveal the knowledge diffuse trajectories of LSCM under different view with main path analysis. This study is unique that it provides a new view to understand the field of LSCM.
Taxonomy of candidate’s selection for prioritized predictive maintenance in maintenance, repairs and overhaul organizations
Authors: Fedorov Roman , Pavlyuk Dmitry 
Published: October 2022

Purpose Research questions: Is there a systemic relationship between different methods of screening candidates for predictive maintenance? How do the goals of a predictive project influence the choice of a dropout method? How do the company’s characteristics implementing the predictive project influence the selection of the dropout method?Design/methodology/approach The authors described and compiled a taxonomy of currently known methods of screening candidate aircraft components for predictive maintenance for maintenance, repairs and overhaul organizations; identified the boundaries of each way; analyzed the advantages and disadvantages of existing methods; and formulated directions for further development of methods of screening for maintenance, repairs and overhaul organizations.Findings The authors identified the relationship between various screening methods by developing the approach proposed by Tiddens WW and supplementing it with economic methods. The authors built them into a single hierarchical structure and linked them with the parameters of the predictive project. The principal advantage of the proposed taxonomy is a clear relationship between the structure of the screening methods and the goals of the predictive project and the characteristics of the company that implements the project.Originality/value The authors of the article proposed groups of screening methods for predictive maintenance based on economic indicators to improve the effectiveness and efficiency of the screening process.
Comparing the VITEK 2 ANC card, species‐specific PCR, and MALDI‐TOF mass spectrometry methods for identification of lactic acid bacteria
Authors: Kim Sung‐Youn , Park Sol Yi , Jin Ji‐Eun , Hong Kyong‐Suk , Kim Dae‐Jung , Kim Yong‐Kyoung , Shin Hee‐Chang , Cho Soon‐Kil , Kang Dong‐Hyun 
Published: October 2022

AbstractLactic acid bacteria (LAB) are not only the most common probiotics in the food and feed industry but are also used as plant probiotics. Therefore, precise identification of LAB at the species level is required. In this study, we compared three different methods, the VITEK 2 ANC card, species‐specific PCR, and MALDI‐TOF MS, to identify six LAB (Lacticaseibacillus casei, Lacticaseibacillus paracasei, Lacticaseibacillus rhamnosus, Lactiplantibacillus plantarum, Lentilactobacillus buchneri, and Limosilactobacillus fermentum) species previously assigned to the genus Lactobacillus that are used as biofertilizers. Twenty‐two strains of six LAB species were analyzed using the VITEK 2 ANC card, species‐specific PCR, and MALDI‐TOF MS, and identification rates at the species level were 45.5%, 95.5%, and 95.5%, respectively. There were cross‐reactions between L. casei and L. parpacasei, and one strain of L. casei could not be identified by these three methods. PCR assays and MALDI‐TOF MS were applicable for LAB identification.Practical ApplicationLAB are the most common probiotics in the food and feed industry, so precise identification and classification of LAB at the species level are required. This study aimed at comparing three different methods for the effective identification of six LAB species: biochemical testing using VITEK 2 ANC card, species‐specific PCR, and MALDI‐TOF MS analysis.