bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete genome of the extensively antibiotic-resistant GC1 Acinetobacter baumannii isolate MRSN 56 reveals a novel route to fluoroquinolone resistance
Authors: Harmer Christopher J , Lebreton Francois , Stam Jason , McGann Patrick T , Hall Ruth M 
Published: March 2022

Abstract

Objectives
To examine the causes of antibiotic resistance in the extensively resistant global clone 1 (GC1) Acinetobacter baumannii isolate MRSN 56 recovered at a US military treatment facility.

Methods
MRSN 56 was sequenced using MinION (Oxford Nanopore) and the reads combined with available Illumina MiSeq data using Unicycler. Acquired resistance genes were identified using ABRicate and their environment examined. ISAba1 and ISAba125 copies were located.

Results
MRSN 56 is ST1IP:ST231Ox:KL1:OCL1 and the complete genome includes four small plasmids, none of which carry resistance genes. The acquired resistance genes were found at four locations in the chromosome in addition to AbaR28 (aphA1, aacC1, aadA1, sul1) in comM. Tn2006 (oxa23, carbapenem resistance) was both in AbaR4 and alone elsewhere. Two copies of Tn7 (dfrA1, sat, aadA1) were identified. One was associated with a 22 852 bp adjacent segment [tetA(B), sul2] derived from the AbGRI1 island, and this novel configuration was designated Tn7+. Tn7+ was incorporated in the position preferred by Tn7, downstream of glmS, by transposition using a sequence in AbGRI1 resembling the Tn7 terminal inverted repeats. Tn7 was found at a secondary site. Fluoroquinolone resistance appears to involve a mutation in gyrA combined with inactivation by ISAba1 of the marR gene in the mar operon and constitutive expression of marA from the promoter internal to ISAba1.

Conclusions
MRSN 56 represents a new sublineage of GC1 lineage 1 with novel features that had not been detected previously. The involvement of the mar operon in fluoroquinolone resistance has not been noted previously.
A Call for the Development of a Sustainable Pest Management Program for the Economically Important Pest Flies of Livestock: a Beef Cattle Perspective
Authors: Smith K V , DeLong K L , Boyer C N , Thompson J M , Lenhart S M , Strickland W C , Burgess E R , Tian Y , Talley J , Machtinger E T , Trout Fryxell R T 
Published: March 2022

AbstractFilth fly pests have a substantial impact on livestock production; annual losses from filth flies were estimated at over US$1.5 billion in 1981. Knowing filth fly management and animal production have changed significantly over the past 40 yr, our objective is to lay the foundation for the development of a transdisciplinary integrated pest management (IPM) approach that considers the economics of controlling flies in animal production, with most of the examples provided towards beef cattle production systems. By performing an in-depth literature review, it is our goal to highlight losses and expenditures associated with the damages caused by these flies, discuss current management strategies for the system, and propose industry needs in terms of research gaps and producer education to enhance sustainable livestock production. Immediate industry needs include: (1) developing dynamic economic thresholds incorporating animal welfare, economics, impacts of chemical use, and climate-related responses; (2) improving monitoring methods to improve surveillance efforts for flies as a system and how all types collectively shape the system; and (3) updating economic loss assessments to account for losses due to animal defensive behaviors, reduced feed efficiency, and unplanned expenditures. While we focus on the beef cattle system in the United States, this paper is meant to provide an argument for research in worldwide livestock production (e.g., dairy, poultry, swine, and small ruminant).
Adverse Neonatal Outcomes Associated With Maternal Sexually Transmitted Infections From a Public Health Clinic Cohort in Southern Brazil
Authors: Dong Huan Vinh , Leng Mei , Kreitchmann Regis , Klausner Jeffrey D , Nielsen-Saines Karin , Yeganeh Nava 
Published: March 2022

Abstract
Pregnant women at public medical centers in Porto Alegre, Brazil, were recruited for a study on screening and treatment of sexually transmitted infections (STIs). STIs were detected in 79 (23%) of 350 pregnant women and were found to be associated with infant low birth weight (adjusted odds ratio 5.8; 95% confidence interval 1.9-18).
Coraco-clavicular endobutton and fiber tape fixation for Neer’s type II unstable lateral clavicular fracture: a case series with literature review
Authors: Dalei Tushar Ranjan , Samal Barada Prasanna , Gupta Asish Kumar , Senapati Rajesh , Sahoo Tarini Prasad 
Published: March 2022

Abstract
Neer’s type II lateral end clavicle fracture is inherently unstable due to coraco-clavicular ligament disruption. Timely surgery can avoid complications of conservative management. Our study is based on open reduction and fixation with double endobutton and fibre tape in 12 patients. Radiological and functional outcome were assessed using Quick DASH score and SPADI score at regular interval. All the fractures achieved both clinical and radiological union. No intra-operative or post-operative complication was noted. Functional out come and range of motion at 1-year follow-up was quite satisfactory. Open reduction and fixation with double endobutton and fibre tape for lateral end clavicle fracture is a quite rewarding surgery with less steep learning curve, economical and enough stable fixations leading to union and good functional outcome without need of re-surgery for implant removal.
Coordination and Harmony in Bilateral Logic
Authors: del Valle-Inclan Pedro , Schlöder Julian J 
Published: March 2022

AbstractIan Rumfitt (2000) developed a bilateralist account of logic in which the meaning of the connectives is given by conditions on asserted and rejected sentences. An additional set of inference rules, the coordination principles, determines the interaction of assertion and rejection. Fernando Ferreira (2008) found this account defective, as Rumfitt must state the coordination principles for arbitrary complex sentences. Rumfitt (2008) has a reply, but we argue that the problem runs deeper than he acknowledges and is in fact related to the challenge of establishing proof-theoretic harmony. We motivate a distinctively bilateral criterion for harmony and show how the bilateralist can meet it. This also resolves Ferreira's complaint.
Collisional and radiative pumping in 22-GHz water masers
Authors: Gray M D , Etoka S , Richards A M S , Pimpanuwat B 
Published: March 2022

ABSTRACT
We briefly discuss the history of pumping schemes for the common 22-GHz maser transition in o-H2O, and note that radiative mechanisms have been considered in the past, as well as the better known collisional pumping mechanisms associated with shock waves. Substantial IR irradiation is indeed destructive to inversion at 22-GHz under a wide range of physical conditions. We identify a small number of transitions, including 22 GHz, that can be pumped by both collisional and radiative schemes, and, through radiative transfer modelling over a grid of values in kinetic temperature, number density, and dust temperature, identify loci in density-kinetic temperature space where these schemes lead to strong inversions. The radiative pumping scheme generally operates at higher dust temperature, and lower kinetic temperature, than the collisional scheme. We identify a small network of transitions that form a radiative pumping scheme for the 22-GHz maser, involving radiative transitions of wavelength approximately 6 $\mu$m. This network is capable of supplying more than 50 per cent of the 22-GHz inversion under typical radiative pumping conditions, and it is consistent with the need for high dust and low kinetic temperatures determined from the modelling. We identify a probable case of radiative pumping in a massive star-forming region from the observation of a positive correlation between the flux densities of 22-GHz H2O masers and 6.7-GHz methanol masers. We discuss possibilities for finding radiatively pumped H2O maser lines in other source types.
Formation of non-base-pairing DNA microgels using directed phase transition of amphiphilic monomers
Authors: Lee Chanseok , Do Sungho , Lee Jae Young , Kim Minju , Kim Sang Moon , Shin Yongdae , Kim Do-Nyun 
Published: March 2022

AbstractProgrammability of DNA sequences enables the formation of synthetic DNA nanostructures and their macromolecular assemblies such as DNA hydrogels. The base pair-level interaction of DNA is a foundational and powerful mechanism to build DNA structures at the nanoscale; however, its temperature sensitivity and weak interaction force remain a barrier for the facile and scalable assembly of DNA structures toward higher-order structures. We conducted this study to provide an alternative, non-base-pairing approach to connect nanoscale DNA units to yield micrometer-sized gels based on the sequential phase transition of amphiphilic unit structures. Strong electrostatic interactions between DNA nanostructures and polyelectrolyte spermines led to the formation of giant phase-separated aggregates of monomer units. Gelation could be initiated by the addition of NaCl, which weakened the electrostatic DNA-spermine interaction while attractive interactions between cholesterols created stable networks by crosslinking DNA monomers. In contrast to the conventional DNA gelation techniques, our system used solid aggregates as a precursor for DNA microgels. Therefore, in situ gelation could be achieved by depositing aggregates on the desired substrate and subsequently initiating a phase transition. Our approach can expand the utility and functionality of DNA hydrogels by using more complex nucleic acid assemblies as unit structures and combining the technique with top-down microfabrication methods.
WashU Epigenome Browser update 2022
Authors: Li Daofeng , Purushotham Deepak , Harrison Jessica K , Hsu Silas , Zhuo Xiaoyu , Fan Changxu , Liu Shane , Xu Vincent , Chen Samuel , Xu Jason , Ouyang Shinyi , Wu Angela S , Wang Ting 
Published: March 2022

Abstract
WashU Epigenome Browser (https://epigenomegateway.wustl.edu/browser/) is a web-based genomic data exploration tool that provides visualization, integration, and analysis of epigenomic datasets. The newly renovated user interface and functions have enabled researchers to engage with the browser and genomic data more efficiently and effectively since 2018. Here, we introduce a new integrated panel design in the browser that allows users to interact with 1D (genomic features), 2D (such as Hi-C), 3D (genome structure), and 4D (time series) data in a single web page. The browser can display three-dimensional chromatin structures with the 3D viewer module. The 4D tracks, called ‘Dynamic’ tracks, animatedly display time-series data, allowing for a more striking visual impact to identify the gene or genomic region candidates as a function of time. Genomic data, such as annotation features, numerical values, and chromatin interaction data can all be viewed in the dynamic track mode. Imaging data from microscopy experiments can also be displayed in the browser. In addition to software development, we continue to service and expand the data hubs we host for large consortia including 4DN, Roadmap Epigenomics, TaRGET and ENCODE, among others. Our growing user/developer community developed additional track types as plugins, such as qBed and dynseq tracks, which extend the utility of the browser. The browser serves as a foundation for additional genomics platforms including the WashU Virus Genome Browser (for COVID-19 research) and the Comparative Genome Browser. The WashU Epigenome Browser can also be accessed freely through Amazon Web Services at https://epigenomegateway.org/.
How Smokers of Menthol Cigarettes and Flavored Cigars Might Respond to FDA’s Proposed Bans
Authors: Yang Yong , Lindblom Eric N , Ward Kenneth D , Salloum Ramzi G 
Published: March 2022

Abstract

Introduction
A ban on menthol cigarettes and flavored cigars may reduce smoking and tobacco-related disparities.

Aims and Methods
We aimed to examine the response of current smokers to a hypothetical ban on menthol cigarettes and flavored cigars. Current smokers were recruited online and reported the alternative products that they may switch to under a hypothetical ban, if they would try to obtain the banned products from illicit channels, and their support to the ban.

Results
51% of current smokers would use nonflavored cigarettes and cigars as alternatives, 45% would switch to flavored heated tobacco products or e-cigarettes or quit smoking. 17% would try to obtain the banned flavored products from illicit markets. A majority of menthol only smokers opposed the ban. Daily smokers would be more likely to switch to nonflavored smoking, to try illicit market products, and were less supportive of the ban. Black smokers would be less likely to switch to nonflavored smoking and were more supportive of the ban. Smokers who used menthol cigarettes only would be more likely to switch to nonflavored smoking, less likely to try illicit market sellers, and were the least supportive of the ban.

Conclusions
In response to a ban of all added flavors for cigarettes or cigars, nearly half of the current smokers would quit smoking, largely by switching to nonsmoking products. However, smokers with more chronic use and those who used only menthol cigarettes would be more likely to switch to nonflavored smoking, diminishing the harm reduction potential. The ban may decrease the relatively higher prevalence of menthol cigarette smoking among Blacks compared with other groups.

Implications
A ban on the sale of menthol cigarettes and flavored cigars may decrease the prevalence of smoking because some current smokers may quit smoking and switch to nonsmoking products. However, smokers with more chronic use and those who used menthol cigarettes only were more likely to switch to nonflavored cigarettes or cigars, diminishing the harm reduction potential of the ban. Black smokers would be more likely to switch to products other than cigarettes and cigars thus decreasing their relatively higher prevalence of smoking compared with other groups.
Osteophytes’ position in subjects with DISH and right-sided aorta: verification of the ‘aortic pulsation protective effect’ theory
Authors: Gliner-Ron Masha , Bercovich Eyal , Herman Amir , Lidar Merav , Militianu Daniela , Eshed Iris 
Published: March 2022

Abstract

Objectives
To validate in a large cohort with right-sided aorta the theory that thoracic right-sided flowing osteophytes in DISH results from a ‘protective’ effect of the pulsating descending left-sided thoracic aorta.

Methods
Chest CTs of patients with DISH and right-sided aorta and controls with DISH and left-sided aorta were evaluated and compared on each intervertebral space (IS) for the location of the aorta (right, left, centre) and the location of the osteophyte relative to the aorta (contralateral, ipsilateral, bilateral).

Results
The study and control cohorts included 31 and 35 subjects, respectively (male 22/9 and female 27/8; median age 64.8/65.3 years; P = 0.86). Osteophytes contralateral to the aorta’s location were recorded in the majority of ISs in both the study and control groups (47% and 60%, respectively; P &gt; 0.05), while ipsilateral osteophytes were recorded in 6.9% and 7.7%, respectively (P = 0.002). Bilateral osteophytes located to the right and the left of the aorta were significantly more prevalent in the study group compared with the controls (17.2% and 5.4%, respectively; P = 0.04).

Conclusions
Aortic pulsation plays an important role in inhibiting the development of osteophytes and results in the majority of contralateral osteophytes on both right-sided and left-sided aortas. However, since both ipsilateral and bilateral osteophytes were not at all rare in both groups, other parameters, which are yet to be established, probably contribute to the location of osteophytes.
Clinical integration of patient-reported outcome measures to enhance the care of patients with SLE: a multi-centre prospective cohort study
Authors: Kasturi Shanthini , Price Lori Lyn , LeClair Amy , Patel Neena , Shetty Shreya , Sheira Dina , Weber Serena , Curtis David , Nowell W Benjamin , Salmon Jane , Terrin Norma , McAlindon Timothy E , Mandl Lisa A 
Published: March 2022

Abstract

Objective
To assess the feasibility and impact of integrating electronic patient-reported outcome measures (PROMs) into the routine outpatient care of patients with SLE.

Methods
We conducted a prospective cohort study, utilizing a mixed-methods sequential explanatory design, of SLE outpatients receiving rheumatology care at two academic medical centres. Participants completed electronic PROMs at enrolment and then prior to their next two routine rheumatology visits. PROM score reports were shared with patients and rheumatologists before visits. Patients and rheumatologists completed post-visit surveys evaluating the utility of PROMs in the clinical encounters. Focus groups of patients and interviews with treating rheumatologists were conducted to further explore their experience utilizing PROMs.

Results
A total of 105 SLE patients and 17 rheumatologists participated in the study. Patients completed PROMs in 159 of 184 encounters (86%), with 93% of surveys completed remotely. Patients reported that PROMs were ‘quite a bit’ or ‘very’ useful (55% of encounters) and beneficial to communication (55% of encounters). In contrast, physicians found PROMs useful (20%) and beneficial to communication (17%) less frequently. There was no significant change in visit length, health-related quality of life or disease activity after implementation of PROMs; however, patient satisfaction improved slightly. Qualitative analyses revealed that patients felt PROMs provided utility primarily by facilitating communication, particularly when physicians discussed the surveys.

Conclusion
The remote capture and integration of electronic PROMs into clinical care was feasible in a diverse cohort of SLE outpatients. PROMs were useful to patients and enhanced their clinical experience primarily by facilitating communication.
A rare breast cancer in a patient with pierced nipples
Authors: Barreto Raquel 
Published: March 2022

ABSTRACT
Paget disease of the breast is a rare form of breast cancer that affects the nipple and areolar complex. Clinicians should have a high suspicion for this condition in patients who fail conservative treatment for benign-appearing dermatologic findings regardless of age or sex. This article describes a patient with whose presumed nipple infection was initially thought to be related to her nipple piercing.
Microsurgical Reconstruction for Reanimation of Foot Dorsiflexion in Children
Authors: Allen Luke C.E. , Bourke Grainne 
Published: March 2022

Summary:
Foot drop is a debilitating condition, which causes physical disability and psychological challenges associated with difficulties walking. We report the indications, novel technique, and successful outcomes of two children (three limbs) who underwent free functional gracilis muscle transfers coaptated to the common peroneal nerve to reanimate active foot dorsiflexion and correct foot drop secondary to loss of the anterior compartment.
An Improvised Approach to Relative Motion Extension Splinting in the Emergency Room
Authors: Miller Jonathan E. , Le Brian Q. 
Published: March 2022

Summary:
Lacerations to the dorsum of the hand are frequently complicated by involvement of extensor tendons. Bedside repair of these injuries in the emergency room decreases time to treatment and avoids operating room expenses and anesthetic associated risks. Optimal outcomes require prompt follow-up and initiation of hand therapy to promote tendon gliding and prevent tethering of scar tissue. Here, we present our improvised relative motion extension splint utilized in treatment of zone five and six extensor tendon lacerations. This orthosis is preferred in isolated extensor tendon injuries that are amenable to primary repair at the bedside in the appropriately compliant and motivated patient. Our design is comprised of readily available supplies in the emergency room setting. Our improvised relative motion extension splint is lighter weight versus a traditional plaster orthosis and frees the patient to engage in activities of daily living with the injured hand on day four following tendon repair. With repeated application we have become facile with this design, which also permits flexibility such as placement of a wrist extension splint component if needed. Relative motion extension splinting is an established method of treatment following extensor tendon repair. Here, we present a straightforward method of fabricating such a device in the emergency room without the availability of thermoplastic materials. Future study will be needed to establish the efficacy of this device versus its thermoplastic counterpart.
The Significant Predictors for Breast, Cervical, Colorectal, or Oral Cancer Screening Intention and Behavior in Taiwan
Authors: Huang Hsiu-Chen , Chang Pei-Chi , Li Sin-Fong , Wang Cing-Ya , Huang Wan-Ting , Chen Wei , Fan Sheng-Yu 
Published: March 2022

Background
Cancer screening can improve outcomes in patients with cancer. Accordingly, under the direction of the National Health Insurance program, the Taiwan government conducts screenings for breast cancer, cervical cancer, oral cancer, and colorectal cancer.

Objective
The aim of this study was to identify the primary predictors of cancer screening intention and behavior at 1 and 6 months after patients are provided information and an invitation by telephone to attend cancer screenings.

Methods
In this prospective longitudinal study, 339 participants meeting the screening criteria were recruited. At baseline, telephone interviews were used to collect information on demographic characteristics, exercise and smoking habits, family cancer history, screening beliefs, and screening intention. Screening behavior was followed up at 1 and 6 months after the telephone interviews.

Results
At baseline, 87.02% of the participants intended to undergo screening, and 31.86% and 63.42% had undergone screening after 1 and 6 months, respectively. The predictors of screening intention were awareness of the screening policy, willingness to learn about screening, and believing in the health benefits of screening. The predictor of screening behavior after 1 month was screening intention at baseline, and the predictors of behavior after 6 months were screening intention, marital status, and belief that cancer is a hereditary disease.

Conclusion
Adults with screening intention tended to undergo cancer screenings.

Implications for Practice
The use of strategies based on screening intention, beliefs, and information can be used to improve participation in cancer screening in Taiwan.
The Crooked Nose: A Practical Guide to Successful Management
Authors: Block Lisa , Pfaff Miles J. , Harris Alexandria , Goldstein Jesse A. , Losee Joseph E. 
Published: March 2022

Learning Objectives:
After studying this article, the participant should be able to: (1) Preoperatively evaluate the patient with a crooked nose. (2) Develop a comprehensive preoperative plan specific to the patient. (3) Effectively “deconstruct” and rebuild the crooked nose to address both aesthetic and functional concerns. (4) Use postoperative techniques to maximize outcomes.

Summary:
Correction of the crooked nose requires a detailed understanding of the relevant surgical anatomy, identification of the deforming forces—both intrinsic and extrinsic—contributing to the deformity, and knowledge of techniques needed to effect the desired change. This continuing medical education article takes the reader through the evaluation and formulation processes to develop a patient-specific plan and provides surgical pearls necessary to get the best results possible. Most importantly, this article emphasizes the need to treat the crooked nose as a reconstructive rather than routine aesthetic operation.