bluuebunny/crossref_metadata_2025_split huggingface dataset:
ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor cell invasion
Authors: Li Lifang , Wang Xuefei , Hu Kai , Liu Xinhua , Qiu Li , Bai Changsen , Cui Yanfen , Wang Biyun , Wang Zhaosong , Wang Hailong , Cheng Runfen , Hua Jialei , Hai Linyue , Wang Mengdie , Liu Miao , Song Zian , Xiao Chunhua , Li Binghui 
Published: November 2022

Abstract
Background
Because of the complexity and heterogeneity, therapeutic effect of breast cancer varies in each subtype, which is classified based on the molecular markers for estrogen or progesterone receptors and human epidermal growth factor 2. Thus, novel comprehensive and precise molecular indicators in breast carcinogenesis are urgently in need.
Methods
The expression of ZNF133 in breast cancer tissues was detected by immunohistochemical staining. The interaction between KAP1 complex and ZNF133 was identified by affinity purification-coupled mass spectrometry. The regulatory mechanisms were validated by luciferase reporter assay and co-immunoprepitation. The target genes of ZNF133 was determinged by chromatin immunoprecipitation-based deep sequencing. Gain-or-loss-of-function assays were used to identify the function and underlying mechanisms of ZNF133 in breast cancer. Cancer cell proliferation, invasion, and tumorigenesis of breast cancer cells were analyzed using cell counting assays, colony formation, transwell, and xenograft tumor models.
Results
Here we report that ZNF133, a zinc-finger protein, is negatively associated with advanced pathological staging and poor survival of breast carcinomas. Moreover, ZNF133 is a transcription repressor, physically associated with the KAP1 complex and transcriptionally represses a cohort of genes including L1CAM that are critically involved in cell proliferation and motility. We demonstrate that the ZNF133/KAP1 complex inhibits the proliferation and invasion of breast cancer cells in vitro and suppresses breast cancer growth and metastasis in vivo through dampening the transcription of L1CAM.
Conclusion
Our study ascertains the value of ZNF133 and L1CAM level in the diagnosis and prognosis of breast cancer, contributes to the deeper understanding of the regulation mechanism of ZNF133 for the first time, and provides a new therapeutic strategy and precise intervention target for breast cancer.
Coronavirus spike protein-specific antibodies indicate frequent infections and reinfections in infancy and among BNT162b2-vaccinated healthcare workers
Authors: Kolehmainen Pekka , Huttunen Moona , Iakubovskaia Alina , Maljanen Sari , Tauriainen Sisko , Yatkin Emrah , Pasternack Arja , Naves Rauno , Toivonen Laura , Tähtinen Paula , Ivaska Lauri , Lempainen Johanna , Peltola Ville , Waris Matti , Kakkola Laura , Ritvos Olli , Julkunen Ilkka 
Published: November 2022

Abstract
The burden of seasonal human coronavirus (HCoV) infections in early childhood and adults has not been well analyzed in longitudinal serological studies. Here we analyzed the changes in HCoV (229E, HKU1, NL63, OC43, MERS, and SARS-CoV-2) spike-specific antibody levels in follow-up serum specimens of 140 children at the age of 1, 2, and 3 years, and of 113 healthcare workers vaccinated for Covid-19 with BNT162b2-vaccine. IgG antibody levels against six recombinant HCoV spike subunit 1 (S1) proteins were measured by enzyme immunoassay. We show that by the age of three years the cumulative seropositivity for seasonal HCoVs increased to 38-81% depending on virus type. BNT162b2 vaccinations increased anti-SARS-CoV-2 S1 antibodies, but no increase in seasonal coronavirus antibodies associated with vaccinations. In healthcare workers, during a one year follow-up, diagnostic antibody rises were seen in 5, 4 and 14% of the cases against 229E, NL63 and OC43 viruses, respectively, correlating well with the circulating HCoVs. In 6% of the HCWs, a diagnostic antibody rise was seen against S1 of HKU1, however, these rises coincided with anti-OC43 S1 antibody rises. Rabbit and guinea pig immune sera against HCoV S1 proteins indicated immunological cross-reactivity within alpha-CoV (229E and NL63) and beta-CoV (HKU1 and OC43) genera.
Microwave-assisted High-Throughput Synthesis of Crystalline Porphyrin-based Two-dimensional Polymers for Neuromorphic Vision Sensor
Authors: Yang Haoyong , Sun Qihao , Liao Zhongquan , Hambsch Mike , Guo Zhecheng , Wu Daheng , Jost Birgit , Zhang Yuejun , Mannsfeld Stefan C. B. , Feng Xinliang , Zhu Xiaojian , Zhang Tao 
Published: November 2022

Abstract
Emulating the biological visual adaptation in machine vision systems typically requires a neuromorphic vision sensor system that exhibits biomimetic light intensity-dependent photoadaptation. Inspired by the biological counterpart, porphyrin-based two-dimensional polymers (p2DPs) are of high interest for adaptative sensor due to their atomic thinness and adjustable charge transfer and trapping under light irradiations. However, the high-throughput synthesis of highly crystalline p2DPs thin films for the device application remains a great challenge because of the slow nucleation and crystallization process in classical interfacial synthesis. Here, we report a microwave-assisted interfacial methodology for the rapid synthesis (~3 mins) of p2DP thin films. The resultant film displays a long-range ordered molecular structure with a lateral size of ~23 cm2 and large crystal domains of ~4 µm as well as controllable thickness (2-40 nm). The p2DP film was then used to fabricate a vision sensor which exhibits a biomimetic light intensity-dependent photoadaptation and habituation behavior. Furthermore, a machine visual system consisting of a 4×8 p2DP-sensor array is developed to implement adaptive image recognition under bright and dim light stimuli, showing an accuracy of over 90%.
Dynamics of multipartite quantum steering for different types of decoherence channels
Authors: Li Wei-Chen , Xiao Ya , Han Xin-Hong , Fan Xuan , Hei Xiao-Bing , Gu Yong-Jian 
Published: November 2022

Abstract
Multipartite quantum steering, a unique resource for asymmetric quantum network information tasks, is very fragile to the
inevitable decoherence, which makes it useless for practical purposes. It is thus of importance to understand how it decays in
the presence of noise channels. We study the dynamic behaviors of genuine tripartite steering, reduced bipartite steering,
and collective steering of a generalized three-qubit W state when only one qubit interacts independently with the amplitude
damping channel (ADC), phase damping channel (PDC) or depolarizing channel (DC). Our results provide the region of
decoherence strength and state parameters that each type of steering can survive. The results show that these steering
correlations decay the slowest in PDC and some non-maximally entangled states more robust than the maximally entangled
ones. Unlike entanglement and Bell nonlocality, the sudden death of reduced bipartite steering and collective steering are
directional. In addition, we fifind that not only one party can be steered by a group system, but also two parties can be steered
by a single system. There is a trade-off between the monogamy relation involving one steered party and two steered parties.
Our work provides comprehensive information about the effect of decoherence on multipartite quantum steering, which will
help to realize quantum information processing tasks in the presence of noise environments.
Moderation of mindfulness on the relationship between anxiety and somatization symptoms in middle-aged and elderly female patients with hypertension：A cross-sectional analysis
Authors: Shen Haifei , Zhang Ri , Zhang XiuJie , Zhang Ying , Liu Yan , Song Wei , Cheng Yunpeng , Yang Minghui , Jiang Yinong , Lu Yan 
Published: November 2022

Abstract
Purpose: To explore the moderation of mindfulness on the relationship between anxiety and somatization symptoms in middle-aged and elderly female patients with hypertension, and provide foundation for the development of more effective mindfulness intervention strategies.
Methods: A total of 109 middle-aged and elderly female patients with hypertension were participated in this cross-sectional study from April to July 2022, who were valid responses to the Five Facet Mindfulness Questionnaire (FFMQ), the General Hospital Anxiety and Depression Scale (GHAD), and the Somatization symptom self-rating scale (SSS). The moderating effect of mindfulness was determined using multi-distance linear regression.
Results: The average quantification of all participants: mindfulness was 123.86 ± 10.49, anxiety was 7.41 ± 3.62, and somatization symptoms was 41.2 ± 9.44. In contrast to the improved level of mindfulness, anxiety (P=0.000) and somatization symptoms (P=0.001) were significantly reduced. Anxiety was positively correlated with somatization symptoms (r=-0.606, P=0.000), while mindfulness level was negatively correlated with both anxiety (r=-0.468, P=0.000) and somatization symptoms (r=-0.439, P=0.000). Moreover, mindfulness had a significant moderating effect on the relationship between anxiety and somatization symptoms (N=109) (B=-0.166, t=2.125, P=0.036). The regulation of mindfulness on anxiety and somatic symptoms was more significant in participants with low mindfulness levels (N=56) (B=0.144, t=2.805, P=0.008), but not in participants with high mindfulness levels (N=53) (B=0.037, t=-0.864, P=0.393).
Conclusion: The moderating effect analysis based on regression analysis showed that mindfulness level had a significant moderating effect on anxiety and somatization symptoms, especially in low mindfulness level.
Intrinsic room-temperature ferromagnetism in a two-dimensional semiconducting metal-organic framework
Authors: Feng Sihua , Duan Hengli , Tan Hao , Hu Fengchun , Liu Chaocheng , Wang Yao , Li Zhi , Cai Liang , Yuyang Cao , Wang Chao , Qi Zeming , Song Li , Liu X. G. , Sun Zhihu , Yan Wensheng 
Published: November 2022

Abstract
The development of two-dimensional (2D) magnetic semiconductors remains a challenging issue in materials science, and plays an important role in the next-generation spintronics devices. Herein, we demonstrate that a 2D semiconducting antiferromagnetic Cu-MOF can be endowed with intrinsic room-temperature ferromagnetic coupling using a ligand cleavage strategy to regulate the inner magnetic interaction within the Cu dimers. Notably, employing the element-selective X-ray magnetic circular dichroism (XMCD) technique, we provide an unambiguous evidence for intrinsic ferromagnetism. Exhaustive structural characterizations confirm that the change of magnetic coupling is caused by the increased distance between Cu atoms within a Cu dimer. Theoretical calculations reveal that the ferromagnetic coupling is enhanced with the increased Cu-Cu distance, which depresses the hybridization between 3d orbitals of nearest Cu atoms. Our work provides an effective avenue to design and fabricate MOF-based semiconducting room-temperature ferromagnetic materials, and promotes their practical applications in next-generation spintronics devices.
Effect of drilling parameters on the hole surface integrity of low alloy steel for nuclear power during BTA deep hole drilling
Authors: Li Binghao , Huang Chuanzhen , Tang Zhengyi , Chen Zhen , Liu Hanlian , Chen Zengtao , Niu Jiahui , Wang Zhen 
Published: November 2022

Abstract
Boring Trepanning Association (BTA) deep hole drilling is widely used in machining tube sheet of steam generator. In order to get a better service integrity, the surface quality after machining is required to be higher. In this paper, the effect mechanism of BTA deep hole drilling on the integrity and quality of the machined surface layer of low alloy steel SA508Gr.3Cl.2 for nuclear power are investigated. The results show that the gradient microstructure can be obtained by BTA drilling on the surface of inner hole, including the recrystallized layer with grain refinement and the plastic deformation layer with high density sub-crystal structure and grain distortion. With the increase of drilling speed and feed rate, the deformation layer thickness will increase. The proportion of low angle grain boundaries (LAGBs) increases with the increase of the depth from the machined surface under each drilling parameter. The increase of drilling speed leads to the increase of recrystallization degree and the proportion of LAGBs in the machined surface. The effect of feed rate on the proportion of LAGBs is opposite. The machined surface is characterized by regular peak and valley, and there are typical surface defects mainly involving feed marks, surface tearing, and ploughing grooves. With the increase of drilling speed, the surface roughness will decrease. The effect of feed rate on surface roughness is obviously lower than that of drilling speed. With the increase of drilling speed and feed rate, the depth of hardened layer increases gradually, which is caused by dislocation strengthening and fine grain strengthening effect during BTA drilling process. Higher drilling speed is recommended in forming a good machined surface with a strengthening layer of a certain thickness.
Numerical modelling and processing experiment of gas-liquid-solid three phase rotary abrasive flow machining
Authors: Tan Yunfeng , Ni Yesha , Wu Jiafeng , Li Lin , Tan Dapeng 
Published: November 2022

Abstract
Enhancing the processing efficiency and surface quality of difficult-to-machine materials such as large-area titaniferous alloy parts is the focus of low-carbon manufacturing. Here, we present a novel microbubble enhancement effect-based three-phase rotary abrasive flow polishing method (RGLSP). The characteristics of the high-speed rotary flow are calculated by the mixture model and modified realizable k-ε turbulence model. The simulation results reveal the dynamic characteristics of the high-speed turbulent vortex flow field formed by the three-phase abrasive flows in the constrained flow passages. The results show that the pitch-down angle of the processing medium inlet is the important parameter that affects the impact distribution features, and there is an optimal value for the impact characteristics of the rotary flow. Then the processing tool and RGLSP experimental platform are established, and the RGLSP method can improve the machining efficiency by 60%. The RGLSP method also can improve the surface quality of material without subsurface damage and the roughness can reach Ra 0.1µm after 150min machining.
Molecular basis for protein histidine N1-specific methylation of the “His-x-His” motifs by METTL9
Authors: Zhao Wentao , Zhou Yang , Li Caiyi , Bi Yucong , Wang Keyun , Ye Mingliang , Li Haitao 
Published: November 2022

Abstract
Histidine methylation serves as an intriguing strategy to introduce altered traits of target proteins, including metal ion chelation, histidine-based catalysis, molecular assembly, and translation regulation. As a newly identified histidine methyltransferase, METTL9 catalyzes N1-methylation of protein substrates containing the “His-x-His” motif (HxH, x denotes small side chain residue). Here our structural and biochemical studies revealed that METTL9 specifically methylates the second histidine of the “HxH” motif, while exploiting the first one as a recognition signature. We observed an intimate engagement between METTL9 and a pentapeptide motif, where the small “x” residue is embedded and confined within the substrate pocket. Upon complex formation, the N3 atom of histidine imidazole ring is stabilized by an aspartate residue such that the N1 atom is presented to S-adenosylmethionine for methylation. Moreover, METTL9 displayed a feature in preferred consecutive and “C-to-N” directional methylation of tandem “HxH” repeats that exist in many METTL9 substrates. Collectively, our work illustrates the molecular design of METTL9 in N1-specific methylation of the broadly existing “HxH” motifs, highlighting its importance in histidine methylation biology.
A case report about a child with drug-resistant tuberculous meningitis
Authors: Tong Jing , Gao Mengqiu , Chen Yu , Wang Jie 
Published: November 2022

Abstract
Background: Hematogenous disseminated pulmonary tuberculosis predisposes to concurrent tuberculous meningitis (TBM), the most devastating and disabling form of tuberculosis. However, children often have atypical clinical symptoms, difficulty in specimen collection, low specimen content, and the increasing incidence of drug-resistant tuberculosis, which makes the accurate diagnosis and timely treatment of childhood tuberculosis face monumental challenges.
Case presentation: The 14-year-old female, was admitted to the hospital with intermittent fever, headache, and blurred vision, her cerebrospinal fluid (CSF) examination showed a lymphocytic pleocytosis, elevated protein level, decreased chloride level, positive for TB-RNA, Xert MTB/RIF detected Mycobacterium tuberculosis, but rifampin resistance test was unknown. Subsequently, her CSF culture was positive for Mycobacterium tuberculosis, and the drug sensitivity test (DST) revealed resistance to isoniazid, rifampin, and fluoroquinolones. Computed tomography (CT) of the chest showed diffuse miliary nodules in both lungs. Intracranial enhanced magnetic resonance imaging (MRI) showed “multiple intensified images of the brain parenchyma, cisterns, and part of the meninges.”The final diagnosis is hematogenous disseminated pulmonary tuberculosis and pre-extensive drug-resistant TBM. After 19 months of full oral individualized antituberculosis treatment, her infection was effectively controlled with no neurological sequelae.
Conclusion: For patients with hematogenous disseminated pulmonary tuberculosis, especially children, even if there are no typical clinical symptoms, it is necessary to know whether there is TBM and other conditions, and constantly look for the relevant aetiological basis to clarify whether it is drug-resistant tuberculosis, to achieve a rapid and accurate diagnosis and timely and effective treatment, which can effectively improve prognosis and reduce mortality and disability rate.
comparison of the diagnostic performance of the ACR and C TIRADS applying the ACR TIRADS’ thresholds for FNAcomparison of the diagnostic performance of the ACR and C TIRADS applying the ACR TIRADS’ thresholds for FNA
Authors: Si Cai-feng , Fu Chao , Cui Yi-yang , Cui Ke-Fei 
Published: November 2022

Abstract
Objective To investigate the diagnostic performances and unnecessary fine needle aspiration (FNA) rates of two point-scale based TIRADS and compare them with a modified version using the ACR TIRADS’ size thresholds.
Methods Our Institutional Review Board approved this retrospective study and waived the requirement for informed consent. A total of 2083 thyroid nodules 10 mm or larger in size in 1779 patients with definitive pathological findings were included. Ultrasonography categories were assigned according to each guideline. We applied the ACR TIRADS’ size thresholds for FNA to the C TIRADS and defined it as the modified C TIRADS (mC TIRADS). Diagnostic performances and unnecessary FNA rates were evaluated for the original and modified guidelines.
Results Of the original guidelines, the ACR TIRADS had higher specificity, accuracy, and area under the receiver operating characteristic curve (AUC) (51.6%, 67.7%, and 0.723, respectively). When the size threshold of the ACR TIRADS was applied to the C TIRADS, the resultant mC TIRADS had higher specificity and accuracy than the ACR (56.1% vs 51.6%,68.7% vs 67.7%). The mC TIRADS had similar unnecessary FNA rate and AUC with the ACR TIRADS (43.7% and 45%, 0.722 vs 0.723，respectively). The false-negative rate of the C TIRADS was the lowest (2.0%) among all TIRADS.
Conclusion The modified C TIRADS incorporating the size thresholds of the ACR showed higher diagnostic performance and a lower unnecessary FNA rate than the original point-scale based TIRADS.
Exploration of the mechanisms affecting ischemic stroke by ceRNA network construction
Authors: Sun Bin , Jia Jun , Yang Hua , Xiang Xin , Liu Xiaoxian 
Published: November 2022

Abstract
Background
Ischemic stroke is among the most common and fatal diseases.. Apoptosis exerts a crucial role in ischemic stroke and heart disorders. The role of the circRNA associated with apoptosis in ischemic stroke was not fully elucidated.
Methods
Three datasets, GSE122709, GSE133768, and GSE110993, from the GEO database, were enrolled in bioinformatics analysis. The targeting miRNAs and mRNAs were predicted through the circBase and miRWalk database. The circRNA-miRNA-mRNA network was created by Cytoscape software. The ‘clusterProfiler’ R package was applied to execute GO and KEGG analysis. We constructed the SH-SY5Y oxygen-glucose deprivation (OGD) model to simulate ischemic stroke in vitro. RT-qPCR and western-blot was utilized to examine the expression level of corresponding genes. Cell Counting Kit-8 was utilized to assess cell viability. Flow cytometry was conducted for cell apoptosis analysis.
Results
Based on the data from public database, we finally created a circRNA-miRNA-mRNA network containing 143 nodes and 272 edges. In the network, the expression of CDKN1A (cyclin dependent kinase inhibitor 1A) was regulated by hsa-miR-17-5p, which is regulated by hsa-circ-0004622. Then, we verified the expression of CDKN1A, miR-17-5p, and circ-0004622 in SH-SY5Y OGD model. The result of functional experiment showed that circ-0004622 promoted apoptosis in ischemic stroke by regulating miR-17-5p.
Conclusion
Taken together, by bioinformatics methods and functional experiments, this study excavated and investigated the role of circ-0004622 in promoting cell apoptosis in ischemic stroke, providing a theoretical foundation for clinical diagnosis and elucidation of the molecular mechanism of ischemic stroke.
Surveillance of N. gonorrhoea in Tamale, Northern Region of Ghana
Authors: Musah Haris Sualah , Addy Francis , Dufailu Osman Adamu 
Published: November 2022

Abstract
The evolution of N. gonorrhoea into a “super bag” and the absence of a viable vaccine calls for continuous surveillance. Surveillance data of N. gonorrhoea in Ghana remains scanty with no known record from the Northern sector of Ghana. In this study, primary swab samples (230) were collected from Tamale metropolis to isolate N. gonorrhoea. Presumptive N. gonorrhoea isolates were confirmed via polymerase chain reaction (PCR) targeting the porB gene. One isolate (0.043%) was molecularly confirmed to be N. gonorrhoea. Further surveillance in Northern Ghana is encouraged.
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in mice
Authors: Reid Kim , Daniels Eileen G. , Vasam Goutham , Kamble Rashmi , Janssens Georges E. , Hu Man , Green Alexander E. , Houtkooper Riekelt H. , Menzies Keir J. 
Published: November 2022

Abstract
Maintaining mitochondrial function is critical to an improved health span and lifespan. Introducing mild stress by inhibiting mitochondrial translation invokes the mitochondrial unfolded protein response (UPRmt) and increases lifespan in several animal models. Notably, lower mitochondrial ribosomal protein (MRP) expression also correlates with increased lifespan in a reference population of mice. In this study, we tested whether partially reducing the expression of a critical MRP, Mrpl54, reduced mitochondrial DNA-encoded protein content, induced the UPRmt, and affected lifespan or metabolic health using germline heterozygous Mrpl54 mice. Despite reduced Mrpl54 expression in multiple organs and a reduction in mitochondrial-encoded protein expression in myoblasts, we identified few significant differences between male or female Mrpl54+/- and wild type mice in initial body composition, respiratory parameters, energy intake and expenditure, or ambulatory motion. We also observed no differences in glucose or insulin tolerance, treadmill endurance, cold tolerance, heart rate, or blood pressure. There were no differences in median life expectancy or maximum lifespan. Overall, we demonstrate that genetic manipulation of Mrpl54 expression reduces mitochondrial-encoded protein content but is not sufficient to improve healthspan in otherwise healthy and unstressed mice.