bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Pretreatment Temperature in The Mycobacterium tuberculosis Extraction Method on DNA Quality
Authors: Setianingsih Tri Yuli , Fitri Safira , Wahyono Daniel Joko , Setiawaty Vivi 
Published: January 2025

Abstract: The existing DNA extraction process for Mycobacterium tuberculosis employs a pretreatment step that may not function at its full potential. This inefficient process led to complications in subsequent analysis; thus, it is necessary to determine the successful extraction method with or without adding a pretreatment step. This study aims to determine the effect of temperature pretreatment on the extraction method of M. tuberculosis on the quality of DNA for WGS examination. The research design was analytically descriptive with a kit-based extraction method from ExiPrepTM Dx Mycobacteria Genomic DNA Kit (K-4418), Bioneer. The results of this study were that DNA purity did not differ (p = 0.959) between the pretreatment heating at 950 °C (2.88) and no heating at 950 °C (2.46). DNA concentration did not differ (p-value = 0.111) between pretreatment heating 950C (0.416 ng/µL) and no heating 950C (0.653 ng/µL). The relatively low DNA concentration resulted in the DNA bands not being visualized on agarose gel electrophoresis (0.8%). In conclusion, our study showed that the pretreatment heating step on M. tuberculosis DNA extraction does not affect DNA purity, concentration, or integrity. This inefficiency step can be discarded from the M. tuberculosis DNA extraction process to simplify the extraction processing. However, other extraction kit approaches need to be explored in future studies.Abstrak: Proses ekstraksi DNA Mycobacterium tuberculosis yang saat ini dilakukan memerlukan pretreatment yang mungkin tidak berpengaruh secara optimal. Proses yang tidak efisiensi berpengaruh terhadap analisis selanjutnya, sehingga perlu untuk mengetahui keberhasilan metode ekstraksi dengan penambahan proses pretreatment. Tujuan penelitian ini adalah untuk mengetahui pengaruh pretreatment suhu pada metode ekstraksi M. tuberkulosis terhadap kualitas DNA untuk pemeriksaan WGS. Desain penelitian adalah deskriptif analitik dengan metode ekstraksi berbasis kit dari ExiPrepTM Dx Mycobacteria Genomic DNA Kit (K-4418), Bioneer. Hasil penelitian adalah kemurnian DNA tidak berbeda (p=0,959) antara pretreatment pemanasan 950C (2,88) dengan tanpa pemanasan 950C (2,46). Konsentrasi DNA tidak berbeda (p value = 0,111) antara pretreatment pemanasan 950C (0,416 ng/µL) dengan tanpa pemanasan 950C (0,653 ng/µL). Konsentrasi DNA yang relatif rendah mengakibatkan pita DNA tidak tervisualisasi pada elektroforesis gel agarosa (0,8%). Kesimpulan dari penelitian ini, bahwa langkah pemanasan awal pada ekstraksi DNA M. tuberkulosis tidak berpengaruh terhadap kemurnian, konsentrasi, dan integritas DNA. Langkah ini dapat dihilangkan dari proses ekstraksi DNA M. tuberkulosis untuk menyederhanakan proses ekstraksi. Namun, pendekatan kit ekstraksi lainnya perlu dieksplorasi dalam penelitian selanjutnya
Students' Perceptions in Using Quizizz Application in Reading Comprehension For Grade 8th Junior High School
Authors: Ummi Khalsum Juriah 
Published: January 2025

Technology has positive effects on education, including active learning in the classroom, collaborative learning, creative learning, and evaluative learning. This is also helped by the expansion of information and communication technology (ICT), which offers several advantages for the development of pupils' language abilities, especially in reading. This study aims to determine the students' perceptions based on students'in using Quizizz Application in reading comprehension at SMPN 23 Tanjung Jabung Timur, Jambi province. The researcher used a quantitative research with descriptive method and employed questionnaire to collect the data. The subjects of this research are 58 students at SMPN 23 Tanjung Jabung Timur. The findings showed that most students have positive perceptions toward Quizizz as an assessment tool. They agreed that Quizizz made them have new experiences in reading comprehension in the English class because the get new information, this Quizizz has complete and flexible features is easy to use, makes students understand the reading comprehension material better. The problems met by students in using Quizizz such as students are disturbed by notifications from other applications when doing exercises through Quizizz, afraid of being constrained by an unstable network, time limits for working on questions that are displayed and according to students' perceptions, not all reading comprehension matches using Quizizz.
PAP Smear versus Colposcopy in the Absence of HPV-DNA Testing for the Screening of Pre-malignant and Malignant Cervical Lesions
Authors: Shrestha Arati , Sunwar Kopila , Shah Miki , Thapa Sunita , Pokharel Aruna , Shrestha Deepak , Tiwari Archana 
Published: January 2025

Introduction: The incidence of cervical cancer is very high in developing countries. The extensive use of cervical screening with PAP smear and colposcopy has considerably increased the detection of premalignant and malignant cervical lesions. Methods: This was a hospital-based study conducted over a period of three months. All the patients underwent gynecological examination, PAP smear and colposcopy. In case of abnormal findings in colposcopy or follow-up cytology, patients were advised for cervical biopsy. With reference to the histopathological reports, the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of PAP smear and colposcopy were evaluated. Results: The most common cytological result was Negative for Intraepithelial Lesion or Malignancy (NILM, 60%) followed by Atypical Squamous Cells of Undetermined Significance (ASCUS, 10%), Atypical Squamous Cells- cannot exclude High grade lesion (ASC-H, 6%), Low grade Squamous Intraepithelial Lesion (LSIL,10%) and High grade Squamous Intraepithelial Lesion (HSIL, 11%). In colposcopy, 60% of the patients were normal and 40% showed atypical transition zone. Histo-pathological findings among 53 patients were NILM (13.2%), cervicitis (13.2%), Cervical Intraepithelial Neoplasia I (CIN I, 28.3%), CIN II and CIN III (39.6%) and invasive carcinoma (5.66%). The sensitivity, specificity, PPV and NPV of PAP smear were 63.6%, 66.6%, 33.3%, and 87.5% respectively. Similarly sensitivity, specificity, PPV, NPV of colposcopy for abnormal cervical lesions were 87.56%, 15.38%, 76.09% and 28.57% respectively. Conclusion: In the absence of HPV-DNA testing, the combined use of PAP smear and colposcopy to detect premalignant and malignant cervical lesions increases early detection and treatment.
Clinical Profile and Short-term Outcome of Heart Failure Patients in a Tertiary Hospital in Kaski, Nepal: A Cross-sectional Study
Authors: Adhikaree Ajay , Budha Arjun Kumar , Rawat Gobind , Nepal Choodamani , Dhungana Umesh , Gautam Suwas Chandra , Bidari Prayas 
Published: January 2025

Introduction: Heart failure is one of the leading causes of hospitalization. The aim of this study was to evaluate the epidemio-clinical profile and short-term outcome of hospitalized heart failure patients in a tertiary care hospital. Methods: This descriptive cross-sectional study was conducted at Pokhara Academy of Health Sciences, Kaski, Nepal from October 1, 2021 to January 31, 2022. All the hospitalized heart failure patients aged 18 years or above were included. Relevant history, examination, laboratory, and pertinent findings were noted. Descriptive statistics were used for qualitative and quantitative data. Paired t-test was used for the comparison of pre-and post-hospitalization data. A p-value <0.05 was taken for statistical significance. Results: There were a total of 116 patients (65.5% females) with a mean age of 64.20 Â± 16.35 years. Most of them had shortness of breath (97.4%) and orthopnea (72.4%) and presented with pedal/sacral edema (81.9%) and bilateral basal crepitations (69.8%) in the chest. Heart failure with preserved ejection fraction was the most prevalent (61.2%) type and dilated cardiomyopathy (27.6%) was the commonest etiology of heart failure. The median duration of hospitalization was five days and the in-hospital mortality was 2.6%. Loop diuretics and vasodilators (angiotensin-converting enzyme inhibitor/angiotensin receptor blocker) were the most commonly used medications. Conclusions: Dilated cardiomyopathy was the most common etiology and heart failure with preserved ejection fraction was the predominant type of heart failure. With a short length of stay and low in-hospital mortality, the short-term outcome was good.
The hazard zone of Mount Merapi and the community’s perception of (in)securities
Authors: Saptaningtyas Haryani 
Published: January 2025

Human beings identify security based on their life experiences and their ability to calculate risk, determining the degree to which risk is acceptable or unacceptable. Living years in a risk-prone area bears the sensitivity that enables adaptation to temporary risks, such as volcanic eruptions. Mount Merapi, located on Java Island, is considered one of the most dangerous volcanoes in the world. In the prone area, the perception of risk has been invented by a legal body, such as the government and Non-Governmental Organizations (NGOs), through hazard mitigation strategies that dominate the discourse. At the same time, the voices of local people are less prominent. Local communities tend to perceive the dichotomy concept of hope and despair in a contextual manner, which influences their sens of uncertainty. The article explores how security and danger are contextualized under specific times and conditions. The empirical study aims to understand the varying perceptions by focusing on translating the term “human security” into the local language. The concept of human security, introduced as an international framework, was introduced using a top-down approach, adopted by the government, and applied at the local level. Thus, managing their own risk, will enhance local security and become active agents in defining risk and formulating their coping strategies.
Analysis of Work Posture with Revised NIOSH Lifting Equation (RNLE) and Mental Workload with Subjective Scale of Mental Workload (ESCAM) in Employees (CV Gajah Printing)
Authors: Asyari Hasyim , Al Hakim Reza Azizul Nasa , Musmuallim M. , Vargas S Rillke Rifyal 
Published: January 2025

Human resources are one of the main elements that need to be considered because of their limited abilities. The limited ability of humans to perform various kinds of physical and non-physical activities is usually influenced by work postures that are less ergonomic and excessive workload. The purpose of this study is to identify the risk of skeletal muscle injury of shopping bag plastic manufacturing operators using NIOSH Lifting, measure the mental workload of shopping bag plastic manufacturing operators using the ESCAM method, and provide suggestions for improvement using the NIOSH Lifting and ESCAM methods. Based on the results of measuring workers' work posture with the RWL and LI equations, it is known that manual handling activities at the printing, blowing, and mixing machine workstations have an LI> 3 value, where these activities have a risk level for skeletal muscle injury. While the results of measuring employee mental workload, 2 factors can affect employee performance, namely the consequences for health and task characteristics.
In vivo measurement of radiation dose during brachytherapy treatment using scintillation detectors
Authors: Sobiech Bogna , Winiecki Janusz , Witkiewicz-Łukaszek Sandra , Zorenko Yuriy , Makarewicz Roman , Wróbel Patrycja 
Published: January 2025

Abstract

Introduction: In vivo verification of the delivered dose in brachytherapy remains an unsolved dosimetric problem. Conventional detectors based on ionization chambers, semiconductors or thermoluminescent (TL) materials based on LiF or Al2O3:C compounds cannot be used for this purpose. The first way to solve this problem is to use dosimetric materials that can be placed in the patient’s body to record the delivered radiation dose in-situ using the OSL and/or scintillation phenomena.

Material and Methods: In this work, it was proposed to use a known scintillation crystal of Ce3+ doped Ga3Ga3Al2O12:Ce garnet (GAGG:Ce) attached with a long optical fiber to measure in situ the radiation dose during brachytherapy treatment. The measurements were first performed in a phantom, and then on patients in the clinical conditions of the Oncology Center in Bydgoszcz.

Results: The obtained results are very encouraging. Luminescence spectra from scintillators were recorded using a long optical fiber and a sensitive spectrometer, which can be placed in a safe place. Measurements on the phantom showed the perfect linear correlation between the dose and signal registered by the detector based on the GAGG:Ce crystal. Meanwhile, clinical measurements are subject to some uncertainty regarding the accuracy of detector placement in the treatment planning system and the possible passive emission response of the optical fiber.

Conclusion: Scintillation crystals combined with optical fibers and very sensitive luminescence spectrometers create an ideal measurement system for in situ determining the dose of various ionizing radiations. However, their use in clinical practice requires the development of procedures for precisely positioning the detector in relation to the radiation target.
Individual dosimetry and monitoring response to treatment in tandem PRRT with [177Lu/90Y]-DOTA-TATE
Authors: Kacperski Krzysztof , Budzyńska Anna , Kubik Agata , Pastusiak Patrycja , Chalewska Wioletta , Lenda-Tracz Wioletta , Borkowska Anna , Walecka-Mazur Agata , Szczodry Artur , Braziewicz Janusz , Kołodziej Maciej , Kamiński Grzegorz , Dedecjus Marek , Długosińska Joanna , Januszkiewicz-Caulier Joanna , Hubalewska-Dydejczyk Alicja , Opalińska Marta , Kowalska Aldona , Mikołajczak Renata 
Published: January 2025

Abstract

Introduction: The Duonen project aims to test the efficacy of tandem PRRT therapy of neuroendocrine tumors with DOTA-TATE labeled with 177Lu and 90Y radionuclides compared to conventional mono-radionuclide therapy using 177Lu with a fixed activity of 7.4 GBq. The paper presents the dosimetric procedure used and preliminary results obtained from the treatment of 40 patients and 113 cycles of therapy.

Material and Methods: Dosimetry is performed using the QDose program based on 4 SPECT/CT scans and 5 blood samples taken for bone marrow dosimetry. Calculation of the dose to the kidneys is done by segmenting them on images, fitting the corresponding activity versus time (TAC) curves and calculating the dose using the Voxel-S method. The dose to the red marrow is calculated based on the IDAC2 model using the TAC curve for blood obtained from the measurements (self-dose) and the segmented organs from images (cross-dose). In addition, the time-integrated activity coefficients of activity in selected tumors or high uptake areas in four consecutive cycles of therapy were calculated for several patients as a measure of response to therapy.

Results: Large variability of individual doses to kidneys is observed. The average specific dose for 177Lu was equal to 0.76 Gy/GBq, SD = 0.29 Gy/GBq, and for 90Y: 3.26 Gy/GBq, SD = 1.26 Gy/GBq. With the standard activity equal to 7.4 GBq in the first cycle, the activity in the next cycle could be increased by > 10% in 60% of cases and had to be decreased in 16% of cases.

Conclusion: Individual dosimetry is an important part of PRRT, leading to significant dose modifications in subsequent cycles in more than 80% of the cases studied.
Penicillin allergy management strategies relevant for clinical practice - a narrative review
Authors: Ghiordanescu Ileana-Maria , Molinari Nicolas , Forsea Ana-Maria , Schrijvers Rik , Hoarau Cyrille , Demoly Pascal 
Published: January 2025

Abstract
Penicillin allergy is the most commonly reported drug allergy, with prevalence rates ranging from 6% to 31% across various populations and geographic areas. The penicillin allergy label is linked to higher mortality and morbidity rates, extended hospital stays, increased readmission rates, and a greater reliance on second-line antibiotics. Research indicates that nearly 99% of those labeled as penicillin-allergic can tolerate the drug. However, alternative antibiotics are often prescribed without confirming the allergy, largely due to legal concerns regarding re-exposure. Even when a negative challenge test is conducted, non-allergist providers may remain hesitant to reintroduce penicillin. To address the considerable gap between reported and actual penicillin allergies, as well as to ensure the prompt use of penicillins by non-allergists, various management strategies have emerged in recent years. Although several comprehensive reviews have examined these strategies, selecting and applying the most suitable for routine practice is difficult. This narrative review focuses on the most relevant data regarding the efficiency of key penicillin allergy risk assessment tools, particularly those of clinical significance, and discusses their readiness for implementation in non-allergist settings.
Göçmen Peyniri Üretiminde Reçete Standardizasyonu, Mikrobiyolojik Kalite ve Duyusal Özelliklerin Değerlendirilmesi
Authors: Saygılı Derya , Özden Onur 
Published: January 2025

Bu çalışmanın amacı, İzmir ili merkez ilçelerinde üretilen Göçmen peyniri üretiminde standart reçetelerin tanımlanmasıdır. Bu amaçla ürünlerin mikrobiyolojik ve duyusal özellikleri değerlendirilerek tüketici profiline hitap eden ve endüstriyel üretime adapte edilecek en uygun standart reçetenin tanımlanması hedeflenmiştir. Araştırmada Balkan göçmeni bireylerden toplanan tarifler değerlendirilerek üretilen 4 farklı ürün için standart reçete çalışması yanı sıra duyusal analiz ve mikrobiyolojik analizler gerçekleştirilmiştir. Lor, Yoğurt, Lor-yoğurt ve Lor-süt şeklinde üretilen ürünlerde tat, koku, kıvam ve genel beğeni özellikleri hedonik skala (1-9) ile değerlendirilmiş ve genel beğeni puanı ortalaması sırası ile 7,63; 4,32; 6,63 ve 7,84 olarak tespit edilmiştir. Benzer şekilde Lor, Yoğurt, Lor-yoğurt ve Lor-süt ile üretilen geleneksel ürünlerde Lactobacillus spp.sayısı sırası ile 8,18; 6,11; 6,41 ve 7,34 kob/g; Laktokok sayısı sırası ile 7,44; 7,51; 7,35 ve 7,36 kob/g ve maya-küf sayısı sırası ile 4,41; 5,61; 4,71 ve 2,85 kob/g olarak tespit edilmiştir. Lor, Yoğurt, Lor-yoğurt ve Lor-süt ile üretilen geleneksel ürünlerde Staphylococcus aureus (S. aureus) sayısı sırası ile 2,06; ˂10; 3,78 ve 2,58 kob/g olarak tespit edilmiştir. Escherichia coli (E.coli) aranan tüm örneklerde ˂10 kob/g olarak bildirilmiştir. Geleneksel ürünler içerisinde fermente süt ürünü olarak önemli olan göçmen peyniri Balkan topraklarından Anadolu’ya taşınan bir kültürel mirastır. Toplumları yansıtan yemek kültürüne sahip çıkmak, gelecek nesillere aktarılmasını sağlamak, gastronomi turizmini canlandırmak amacı ile standart reçete çalışmalarının yerel lezzetler üzerine yoğunlaşması önem arz etmektedir.
Evapotranspiração, coeficiente do cultivo e eficiência e uso da água da berinjela sob diferentes lâminas de irrigação deficitária em região semiárida
Authors: DANTAS SÍLVIA MARIA , De Paulo Rodrigues Vicente , Tavares Silva Madson , Kaori Matsunaga Wendy , De Barros Santiago Dimas , PAULO FERREIRA FRANCISCO EDSON , MIRANDA SILVA EUCLIDES 
Published: January 2025

A berinjela é uma importante cultura na produção das hortaliças em diversas regiões do mundo. Objetivo desta pesquisa foi determinar o coeficiente de cultivo, a evapotranspiração da cultura por meio do balanço hídrico do solo, eficiência e uso da água para a berinjela. O experimento foi realizado em duas safras (chuvosa e seca) e foram distribuídos em dois blocos, para determinar os efeitos do cultivo em sequeiro e o efeito do estresse hídrico com lâminas de irrigação para os tratamentos de 100% (T1), 80% (T2), 60% (T3), 40% (T4) e 20% (T5) de evapotranspiração de referência (ETo) para reposição hídrica diária. Verificou-se os valores do coeficiente de cultivo para o solo franco arenoso 0,63, 0,98,1,10,0,92 e para o solo areia franca foram 0,53,0,68,1,05,0,79 para as fases inicial, vegetativa, produção e maturação, respectivamente. A evapotranspiração da cultura variou de acordo com a disponibilidade hídrica no solo, a eficiência e uso da água foi significativa para o tratamento com maior produtividade T1BI com 15,2 kg m-3 e no T1BII com 8,5 kg m-3. A maior produtividade para o ciclo em sequeiro foi de 9,0 kg m2 e, para o ciclo irrigado os tratamentos T1BI com 5,0 kg m2 e T2BI com 4,0 kg m2, T1BII com 3, 0 kg m2 e T2BII com 2,1 kg m2. As lâminas de irrigação mais eficientes na estimativa da produção para os tratamentos foram T1BI com 51 ton h-¹ e T1BII com 28 ton h-¹, e o T2BI com 20 ton h-¹ e T2BII com 39 ton h-¹ de berinjelas em região de clima Semiárido.
Study on Pull-Up Behavior of Double Fold Anchor with Field Full Scale Test
Authors: Hendrawan Agus J. , Harianto Tri , Djamaluddin A. R. , Muhiddin Ahmad B. 
Published: January 2025

Several studies have been conducted on the use of anchors, including numerical analysis, experimental testing, and field-scale testing. These studies have provided insights into anchor behavior in terms of pull-up capacity and soil failure models under tensile loading. Specifically, for the use of anchors in cohesive or soft soils, it is possible to innovate by using anchor elements with various dimensional or surface area changes. This research aims to design anchors for cohesive soils that can be easily applied in the field and have high tensile capacity, determine the pull-up capacity of double-fold type ground anchors, and analyze the effect of the depth of double-fold anchors. The results of pullout and tensile capacity testing on double-fold anchors showed significant variations at each test location. At the first location, Sungai Kariango, high tensile capacity occurred at relatively shallow embedment depths, influenced by the type and bearing capacity of the soil at the test site. At the second location, although the soil was relatively soft, the tensile capacity was similar to the first location but with deeper embedment depths. At the third location, the consistency of soil type and soil strength at the two test points resulted in similar tensile capacities. This indicates that the type and strength, or bearing capacity, of the soil at the test site, as depicted by cone resistance parameters (qc), significantly affect the tensile capacity of the anchor. The better the soil strength and bearing capacity at the test site, the greater the tensile capacity of the anchor that can be achieved. A deep understanding of soil characteristics through CPT is essential in determining the design and embedment depth of anchors to achieve optimal tensile capacity. Through this research, it is expected to obtain optimal tensile capacity results for anchors and develop a double-fold type ground anchor model that is easy to install in the field, suitable for various structures with high tensile loads, and susceptible to uplift in soft soil layers. Doi: 10.28991/CEJ-2024-010-12-012 Full Text: PDF