bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of NSCLC Cell Proliferation By MARCH7 Via the NF-κB and Wnt/β-Catenin Signaling Pathways
Authors: Lu Xiaomi , Shao Lili , Qian Ye , Zhong Sixun , Chen Jinhong , Yan Aiting , Zhuang Zhixiang , Zhang Yan 
Published: January 2022

Abstract
The aim of the study was to explore the role of the E3 ubiquitin ligase MARCH7 in the development of non-small-cell lung cancer (NSCLC) and to explore the underlying molecular mechanism.Western blot and immunohistochemistry results showed that the expression of MARCH7 in NSCLC cancer tissues was higher than that in paracancerous tissues. Tissue microarray staining results and clinicopathological parameters of NSCLC patients revealed that MARCH7 expression was closely related to TNM stage, degree of tumor differentiation and lymph node metastasis of NSCLC patients. Furthermore, univariate and multivariate analyses and survival curve analysis showed that high expression of MARCH7 was associated with poor prognosis.In vitro, siRNA was constructed and transfected into A549 cells to inhibit the expression of MARCH7. The CCK-8 assay indicated that the growth rate of tumor cells in the interference group was reduced. The number of colonies and cells in the interference group decreased in the plate clone formation experiment. Flow cytometry showed that G0/G1 phase cells were predominantly increased after blocking endogenous MARCH7 expression, and G0/G1 phase arrest occurred in A549 cells. The reporter gene activity of the NF-κB signaling pathway and Wnt/β-catenin signaling pathway was reduced, as validated by a double luciferase reporter gene assay. Western blot analysis showed that the expression of NF-κB P50, NF-κB P65 and β-catenin was decreased, while the expression of E-cadherin was elevated.In vivo, MARCH7-overexpressing virus was constructed and transfected into A549 cells and then subcutaneously injected into nude mice. It was demonstrated that the tumor volume was significantly larger in the MARCH7 overexpression group than in the control nude mice during the same period. Elevated expression of PCNA and Ki-67 was observed in the tumor mass of the MARCH7 overexpression group, as measured by immunohistochemical analysis, accompanied by enhanced levels of NF-κB P50, NF-κB P65 and β-catenin, as detected by Western blot. These results provide a new idea for the experimental basis for the treatment of NSCLC in the future.
The Impact of Mixed-use Development, Small Businesses, and Walkability on Carbon Emissions in Cool Climate Cities
Authors: Zagow Maged 
Published: January 2022

Abstract
United States (US) cities of cool climate zone such as Chicago and Boston are witnessing a reduction in carbon emissions potentially due to focusing on public transportation, and alternative energy resources. It's difficult to validate or deny optimal practices and regulations due to a lack of reliable data on carbon emissions and urban comparative studies amongst metropolitan areas. Therefore, we have examined at the relationship between land use, walkability, socioeconomics variables and carbon dioxide emissions at the zip code level. The current study compares the carbon footprints of four metro regions in cool climatic zone 5 with a model of all US zip code, to generate a benchmarking predictive model for climate change across all US zip codes. Our research shows that increasing number of businesses within walkable distance in cool climate reduces CO2 emissions. This signifies that enhancing walkability in cities and remodeling of retail, art, entertainment, and recreation facilities in accordance with urban sustainability policies can greatly cut down CO2 emissions.
Comparative Genomics Revealed Genus Specific Encoding of Amino Acids by Tri-Nucleotide SSRs in Human Pathogenic Streptococcus and Staphylococus Bacteria
Authors: Mahfooz Sahil , Narayan Jitendra , Ahmed Ruba Mustafa Elsaid , Hag Amel Bakri Mohammed El , Mohammed Nuha Abdel Rahman Khalil , Kausar Mohd Adnan 
Published: January 2022

Abstract
Pathogenic bacteria use phase variation of surface molecules and other characteristics as a significant adaptation mechanism. Repetitive sequences made up of numerous identical repeat units can be found in many phase variable genes. Here, we investigated the frequency and distribution of long-SSRs in 15 human pathogenic Staphylococcus, Streptococcus, and Enterococcus bacteria. Long-SSRs were found to be distributed differently in the genic and intergenic sequences. In the genic sequences, 61.3 SSRs were discovered on average, while 16.2 SSRs were found in the intergenic regions. Staphylococci exhibited the highest frequency of SSRs, followed by Enterococcus, and Streptococci had the lowest frequency of SSRs. Higher A+T content was found to be the best predictor of long-SSR in these human pathogens. Tetranucleotide repeats predominated in intergenic regions, while trinucleotide repeats predominated in genic regions. In human pathogenic Streptococcus and Staphylococcus bacteria, genus-specific encoding of amino acids by tri-nucleotide SSRs was observed. A genetic relationship between these human pathogenic bacteria was derived based on the presence of SSRs in the housekeeping genes and compared to the phylogeny generated based on the 16S ribosomal RNA gene.
Indigenous Knowledge On Medicinal Plants Practiced At Myagdi District, Nepal
Authors: Poudel Anit 
Published: January 2022

Abstract
Indigenous knowledge on medicinal plants and practices is outdistancing and vulnerable to loss if not properly documented. A survey on medicinal plants and their practices was carried in the Myagdi district. Around 40 tribal people from four different villages were interviewed using a semi-structured questionnaire. Documentation of the indigenous knowledge was done in written form and the pictures of available medicinal plants were taken. The study showed that 93.51% of the respondent has used medicinal plants and 87.20% found them effective in curing several diseases and injuries. They are commonly used to cure diseases like bone fractures, abdominal pain, fever, common cold, dysentery, eye opacity, scabies, worm infection, reproductive problems, mental disorder, and cardiovascular problems. As reported, with access to modern pharmaceuticals, the use of medicinal plants has been less practiced these days. The knowledge on medicinal plants and practices are restricted to older-aged groups of the community (76.32%) reflecting that valuable indigenous knowledge is on the wane. This study portrays the commonly used medicinal plants along with their preparation techniques practiced in the study area.
DNA Methylation and Transcriptomic Features are Preserved Throughout Disease Recurrence and Chemoresistance in High Grade Serous Ovarian Cancers
Authors: Gull Nicole , Jones Michell , Peng Pei-Chen , Coetzee Simon , Silva Tiago , Plummer Jasmine , Reyes Alberto , Davis Brian , Chen Stephanie , Lawrenson Kate , Lester Jenny , Walsh Christine , Rimel Bobbie , Li Andrew , Cass Ilana , Berg Yonatan , Govindavari John Paul , Rutgers Joanna , Berman Benjamin , Karlan Beth , Gayther Simon 
Published: January 2022

Abstract
Background

Little is known about the role of global DNA methylation in recurrence and chemoresistance of high grade serous ovarian cancer (HGSOC). We performed whole genome bisulfite sequencing and transcriptome sequencing in 62 primary and recurrent tumors from 28 patients with stage III/IV HGSOC, of which 11 patients carried germline, pathogenic BRCA1 and/or BRCA2 mutations.
Results

Landscapes of genome-wide methylation (on average 24.2 million CpGs per tumor) and transcriptomes in primary and recurrent tumors showed extensive heterogeneity between patients but were highly preserved in tumors from the same patient. We identified significant differences in the burden of differentially methylated regions (DMRs) in tumors from BRCA1/2 compared to non-BRCA1/2 carriers (mean 659 DMRs and 388 DMRs in paired comparisons respectively). We identified overexpression of immune pathways in BRCA1/2 carriers compared to non-carriers, implicating an increased immune response in improved survival (P=0.006) in these BRCA1/2 carriers.
Conclusions

These findings indicate methylome and gene expression programs established in the primary tumor are conserved throughout disease progression, even extensive chemotherapy treatment, and that changes in methylation and gene expression are unlikely to serve as drivers for chemoresistance in HGSOC.
Identification of biomarkers related to metabolically unhealthy obesity in Korean obese adolescents: A cross-sectional study
Authors: Jeong Sarang , Jang Han Byul , Kim Hyo-Jin , Lee Hye-Ja 
Published: January 2022

Abstract
BackgroundObesity is classified as metabolically unhealthy obesity (MUO) and metabolically healthy obesity (MHO). The current study aimed to screen for relationships and different potential metabolic biomarkers involved between MHO and MUO in adolescents.MethodsThe study included 148 obese adolescents aged between 14 and 16. The study participants were divided into MUO and MHO groups based on the age-specific adolescent metabolic syndrome (MetS) criteria of the International Diabetes Federation. The current study was conducted to investigate the clinical and metabolic differences (AbsoluteIDQ™ p180 kit) between adolescents in the MHO group and those in the MUO group. Multivariate analyses were conducted to investigate the metabolites as independent predictors for the odds ratio and the presence of the MetS in adolescents.ResultsThere were significant differences in the 3 acylcarnitines, 5 amino acids, glutamine/glutamate ratio, 3 biogenic amines, and 2 glycerophospholipids between the obese adolescents in the MUO group and those in the MHO group. Moreover, several metabolites were associated with the prevalence of MUO in adolescents. Additionally, several metabolites were inversely correlated with MHO in adolescents of the MUO group.ConclusionsWe observed that histidine, lysine, PCaaC34:1, and several clinical factors in adolescents of the MUO group were reverse correlated with the results in adolescents of the MHO group. In addition, the triglyceride-glucose index was related to MUO in adolescents, compared with the homeostasis model assessment of insulin resistance. Thus, the biomarkers found in this study have a potential to reflect the clinical outcomes of MUO in adolescents. These biomarkers will lead to a better understanding of MetS in obese adolescents.
TYRO Protein Tyrosine Kinase-binding Protein Predicts Favorable Overall Survival in Osteosarcoma and Correlates With Antitumor Immunity
Authors: Xu Hai-ru , Chen Jun-jie , Shen Jin-ming , Ding Wei-hang , Chen Jie 
Published: January 2022

Abstract
Objective: To explore the prognostic significance and underlying mechanism of TYRO protein tyrosine kinase-binding protein (TYROBP) in osteosarcoma. Methods: Firstly, the expression of TYROBP was analyzed using the t-test. The Kaplan-Meier plotter analysis and a receiver operating characteristic (ROC) curve were performed to evaluate the influence of TYROBP on overall survival (OS). Further, Cox regression analysis was conducted to predict the independent prognostic factors for OS of osteosarcoma patients, and a nomogram was constructed. Then, the relationship between TYROBP and clinicopathological characteristics was determined using statistical methods. Enrichment analyses were conducted to evaluate the biological functions of TYROBP. Finally, ESTIMATE algorithm was used to assess the association of TYROBP with immune cell infiltration. Results: TYROBP was significantly increased in osteosarcoma (all P <0.001). However, the high expression of TYROBP was related to better OS of osteosarcoma patients. Cox regression analysis showed that TYROBP was an independent prognostic factor for predicting OS (P =0.005), especially in patients with male sex, age <18 years, metastasis, and tumor site leg/foot (all P <0.05). Besides, TYROBP mRNA expression was significantly associated with tumor site (P <0.01) but had no remarkable relationship with age, gender, and metastasis status (all P>0.05). Functional annotation and GSEA revealed that TYROBP was mainly involved in immune-related pathways. Importantly, TYROBP positively correlated with immune scores (P <0.001, r=0.87). Conclusions: TYROBP served as an independent prognostic biomarker for OS in osteosarcoma. High TYROBP expression might prolong the survival of osteosarcoma patients mainly through promoting antitumor immunity.
Prediction of Shear Strength of UHPC Beam with Small Shear Span to Depth Ratios Based on Modified MCFT
Authors: Zhou Linyun , Wan Shui 
Published: January 2022

Abstract
Ultra-high performance concrete (UHPC) has been gradually used in structure engineering due to its excellent mechanical performance, however, predicting the shear capacity of the UHPC beams is still a challenge, especially for the beams with small shear span to depth ratios. To address this issue, this paper devotes to developing a rational model to predict the shear capacity of the UHPC beams with stirrups based on the modified compression field theory (MCFT) and plastic theory. The shear force will be balanced by the stirrups, matrix, fibers and shear compression zone. The contribution of stirrups, matrix and fibers on shear capacity can be predicted by MCFT, and the contribution of compression zone is determined based on plastic theory. 12 UHPC beams was designed and tested to validate the proposed model. It can be found that the predictions agree well with test results, while the current design codes, including SETRA-AFGC and SIA, give overly conservative values for UHPC beams when the shear to span is less than 2.5.
Gastrointestinal Helminth Parasites of Wild Ungulates In Hirpora Wildlife Sanctuary, Kashmir, India
Authors: Bhat Rouf Ahmad , Tak Hidayatullah , Bhat Bilal A. , Ahmad Riyaz , Dar Jahangir Ahmad 
Published: January 2022

Abstract
Parasitic infection represents an emerging threat to wild ungulates and a challenge to their management. Although a lot of work has been carried out on helminth parasitic infestation of domestic ungulates of Kashmir but the data pertaining to this aspect of wild ungulates has being ignored. The study on gastrointestinal helminth parasitic infestation of wild ungulates was carried out during post livestock grazing period (November to May) of 2018/2019 in Hirpora Wildlife sanctuary (HWLS) to fill the gap in the existing literature. During the study fresh faecal samples of musk deer Moschus sp.(n=44) and markhor Capra falconeri (n=41)were collected and examined qualitatively and quantitatively for gastrointestinal helminth parasites. A total of seven helminth parasites were recorded which are arranged in the descending order of their overall prevalence as Haemonchus spp. (44.70%),Nematodirus spp. (40%), Trichuris spp. (37.64%), Strongyloides spp. (34.11%)Trichostrongylus spp. (28.23%),Monieziaspp. (23.52%) and Fasciola spp. (20%). The mean EPG (eggs per gram) of different parasites showed a considerable variation in both the wild ungulates. The highest mean EPGwas that of Haemonchus spp. and the lowest mean EPG was that of Fasciola spp. in both hosts. A statistically significant difference was observed in the mean EPG of different parasites among two wild hosts (t=3.606, p=0.01).
Giant magnon spin conductivity approaching the two-dimensional transport regime in ultrathin yttrium iron garnet films
Authors: Wei Xiangyang , Santos Obed Alves , Lusero Cristhian Humberto Sumba , Bauer Gerrit , Youssef Jamal Ben , Wees Bart van 
Published: January 2022

Abstract
Conductivities are key material parameters that govern various types of transport (electronic charge, spin, heat etc.)
driven by thermodynamic forces. Magnons, the elementary excitations of the
magnetic order, flow under the gradient of a magnon chemical
potential in proportion to a magnon (spin)
conductivity σm. The magnetic insulator yttrium iron
garnet (YIG) is the material of choice for efficient magnon spin transport. Here we
report an unexpected giant σm in record-thin YIG films
with thicknesses down to 3.7 nm when the number of occupied two-dimensional (2D) subbands is
reduced from a large number to a few, which corresponds to a transition from 3D to 2D magnon transport. We extract a 2D spin conductivity (≈1 S) at room temperature, comparable to the (electronic) spin conductivity of the high-mobility two-dimensional
electron gas in GaAs quantum wells at millikelvin temperatures.
Such high conductivities offer unique opportunities to develop low-dissipation magnon-based
spintronic devices.
Study On The Application of Preoperative Three-Dimensional CT Angiography of Perigastric Arteries In Laparoscopic Radical Gastrectomy
Authors: Liu Peng , Yu Wenbin , Wei Meng , Sun Danping , Zhong Xin , Ouyang Jun , Liang Yize 
Published: January 2022

Abstract
Objection: To investigate the clinical value and significance of preoperative three-dimensional computerized tomography angiography (CTA) in laparoscopic radical gastrectomy for gastric cancer.Methods: The clinical data were analyzed retrospectively from 214 gastric cancer patients. We grouped according to whether to perform CTA. The gastric peripheral artery was classified according to CTA images of patients in the CTA group, and we compared and analyzed the difference of the data between the two groups.Results: The celiac trunk was classified according to Adachi classification: Type I (118/125, 94.4%),Type II (3/125, 2.4%),Type III (0/125, 0%),Type IV (1/125, 0.8%),Type V (2/125, 1.6%),Type VI (1/125, 0.8%).Hepatic artery classification was performed according to Hiatt classification standard:Type I (102/125, 81.6％),Type II (9/125, 7.2%),Type III (6/125, 4.8%),Type IV (2/125, 1.6%),Type V (3/125, 2.4%),Type VI (0, 0%),Others (3/125, 2.4%).And this study combined vascular anatomy and clinical surgical risk to establish a new splenic artery classification model. It was found that the operation time and estimated blood loss in the CTA group were significantly lower than those in the non-CTA group. In addition, the blood loss in the CTA group combined with ICG (Indocyanine Green) labeled fluorescence laparoscopy was significantly less than that in the group without ICG labeled. Conclusion: Preoperative CTA can objectively evaluate the vascular course and variation of patients, and then avoid the risk of operation, especially in combination with ICG labeled fluorescence laparoscopy, can further improve the quality of operation.
Assessment of factors affecting flicker ERGs recorded with RETeval from data obtained from health checkup screening
Authors: Inooka Taiga , Kominami Taro , Yasuda Shunsuke , Koyanagi Yoshito , Ota Junya , Okado Satoshi , Ito Yasuki , Terasaki Hiroko , Nishiguchi Koji M. , Ueno Shinji 
Published: January 2022

Abstract
The purpose of this study was to determine the ocular, sex- and age-specific, anthropometric, and hematologic factors that affect the implicit times and amplitudes of the flicker ERGs recorded with the RETeval system from individuals 40- to 89-years-of-age. Flicker ERGs were recorded with the RETeval system from 330 individuals who had normal fundus and OCT images. Univariate and multivariate regression analyses were performed to identify factors associated with the implicit times and amplitudes of the RETeval flicker ERGs. Univariate regression analyses showed significant correlations between the implicit times and the BCVA, age, axial length, blood sugar level, and BUN in both eyes. Multivariate regression analyses identified age and axial length as two independent factors that were significantly correlated with the implicit times of the RETeval flicker ERGs. Univariate regression analyses also showed significant correlations between the amplitudes and age, platelet count, HDL level, and creatinine level in both eyes. However, smoking habits, body mass index, and blood pressure were not correlated with the RETeval flicker ERGs. We conclude that age and some ophthalmologic and hematologic findings except for anthropometric findings were suggested to significantly affect the measurements of the RETeval flicker ERGs.
Development of Plant-Mediated Silver Nanoparticles &amp; Their Pharmacological Evaluation
Authors: Kalakotla Shanker , P Geetha , Banu Ahmadi , Shaik Shamshe 
Published: January 2022

Abstract
Diabetes is among the most common debilitating and non-transferable diseases on the planet. The idea of using nanoparticles as a drug to treat diabetes mellitus seems intriguing. The Ag nanoparticles (Ag NPs) were effectively produced utilizing Moringa olifera (family: Moringaceae) plant extract employing a simple, cheaper, faster, and environmentally friendly green synthesis process. The antidiabetic effect of the produced Ag NPs was also tested in vivo. In the presence of plant extract, silver nitrate was converted to silver ions (Ag). XRD, FTIR, UV, XPS, and HRTEM studies characterize the formed Ag NPs. Ag NPs that have been biosynthesized, crystal nature was confirmed through XRD analysis and confirmed by UV-visible spectroscopy. FT-IR spectra were used to verify the presence of various functional groups in the biomolecules, forming and stabilizing the nanoparticles. The size of the NPS was in the range of 20-40 nm determined by HRTEM. The induction of diabetes using STZ showed increased blood glucose, cholesterol, triglycerides, LDL, VLDL, massive loss in body weight. These changes were reversed following the treatment of diabetic rats for 28 days and showed significant inhibition (p < 0.001) at a dose range of 0.2 mg/kg leaf extract and 0.2 mg/kg Ag NPs compared with the extract-treated group. These obtained results suggested that plant-mediated Ag NPs have shown promising antidiabetic and anti-hyperlipidemic activity compared to the crude extract.
Dissociation Mechanics and Stability of Type a Botulinum Neurotoxin Complex by Means of Biophysical Evaluation
Authors: Hada Shavron , Lee Jae Chul , Lee Eun Chae , Ji Sunkyong , Nam Jeong Sun , Yun Bum Jin , Na Dong Hee , Kim Nam Ah , Jeong Seong Hoon 
Published: January 2022

Abstract
Biophysical characterization of type A botulinum neurotoxin (BoNT/A) complex along with its thermodynamic stability was assessed through a combination of various methods. BoNT/A exists as large complexes in association with neurotoxin associated proteins (NAPs). To evaluate its biophysical behavior, size-exclusion chromatography (SEC), multi-angled light scattering (MALS), enzyme linked immunosorbent assay (ELISA), and dynamic light scattering (DLS) were utilized. Initially, a single peak (peak 1) of SEC was observed at pH 6.0, and an additional peak (peak 2) appeared at pH 7.4 with a decrement of peak 1. Through MALS and ELISA, the peak 2 was determined to be BoNT/A dissociated from its complex. The dissociation was accelerated by time and temperature. At 37°C, dissociated BoNT/A self-associated at pH 7.4 in the presence of polysorbate 20. On the other hand, the dissociation was partly reversible when titrated back to pH 6.0. Overall, BoNT/A was more stable when associated with NAPs at pH 6.0 compared to its dissociated state at pH 7.4. The conventional analytical methods could be utilized to relatively quantify its amount in different formulations.