bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parental and child adjustment to amyotrophic lateral sclerosis: transformations, struggles and needs
Authors: Sommers-Spijkerman Marion , Rave Neele , Kruitwagen-van Reenen Esther , Visser-Meily Johanna M. A. , Kavanaugh Melinda S. , Beelen Anita 
Published: March 2022

Abstract
Background
Amyotrophic lateral sclerosis (ALS), progressive muscular atrophy (PMA) and primary lateral sclerosis (PLS), together referred to as ALS, are life-limiting diagnoses affecting not only patients but also the families surrounding them, especially when dependent children are involved. Despite previous research highlighting the vulnerability of children in these families, they are, as yet, often overlooked in healthcare. Efforts are needed to better support children in families living with ALS, both directly and through strengthening parents in their parental role. This study sought to gain a better understanding of parental and children’s experiences, struggles and support needs in families living with ALS.

Methods
Semi-structured interviews were conducted with 8 parents with ALS, 13 well parents and 15 children, together representing 17 families. Interview data were analyzed using qualitative content analysis.

Results
Three major themes were identified relating to (1) ALS-related transformations in families’ homes, activities, roles and relationships, that trigger (2) distress among families, which, in turn, evokes (3) emotional, psychological, educational and practical support needs. For emotional and practical support, parents and children mainly rely on their own family and social network, whereas they seek educational and psychological support from healthcare professionals.

Conclusions
Our findings imply that ALS care professionals may foster family adjustment to living with ALS, most notably through encouraging parents to engage in a dialogue with their children about the many transformations, struggles and needs imposed by ALS and teaching them how to start the dialogue.
A review on the socio-economic impacts of informal transportation and its complementarity to address equity and achieve sustainable development goals
Authors: Tucho Gudina Terefe 
Published: March 2022

AbstractSub-Saharan suffers from a poor transport system failing to address equity and sustainability. The system can be affected by several factors; however, this study explores how African historical transport infrastructure development and socio-economic changes affect institutional changes and preferences of available transport modes. We used recently published systematic reviews, case studies, analytical cross-sectional studies, policy review papers, and available relevant studies reports and some grey literature to understand the impacts of these factors. Our analysis shows that the transport demand is affected by local socio-economic contexts shaped by historical pretexts, socio-technical changes, economic transitions, and related value systems. These is essential to understand the operational characteristics of the formal and informal transport modalities, their roles and contribution to meet the mobility needs of the people and designing of an effective management system. This paper can provide insights to policy-makers, urban transport planners and researchers in the field on the complementary modalities involving formal and informal transportation.
Concept maps as a novel assessment tool in medical education
Authors: LOIZOU STELLA , Nicolaou Nicoletta , Pincus Bridget Anne , Papageorgiou Alexia , McCrorie Peter 
Published: March 2022

Background: We conducted a pilot study to investigate the use of Concept Maps (CMs) in a Problem-Based Learning (PBL) setting as a complementary tool to current educational techniques for enhancing medical student knowledge and critical thinking. We also introduced a measure from the field of graph theory as an objective means of CM quality assessment. Methods: Participants were first-year medical students with no or minor prior CM experience. All participants completed questionnaires (demographic information and assessment of learning style) to establish a baseline measure against which the change in clinical and critical thinking was assessed. They were asked to prepare CMs for three PBL cases, and following the submission of the CMs they completed semi-structured critical and clinical thinking questionnaires. A clinical expert also created corresponding “benchmark” CMs for comparison. Qualitative (Wordclouds) and quantitative (graph theory) analysis provided a summary of the key concepts and quantified the CM quality respectively, compared to the “benchmark” CMs. Results: Questionnaires revealed that CMs helped students recall information, organize material in a concise manner, prepare better for their PBL session and provided a good revision tool. It was also found that graph-theoretical measures (graph density, modularity) were suitable for objectively distinguishing between CMs that captured more in-depth knowledge, compared to CMs that contained simpler associations. Conclusions: We have shown that it is possible to quantify CM quality using graph-theoretical measures, such as graph density and modularity.
Fly species on cows around the Tangkoko Nature Reserve, North Sulawesi, Indonesia and their role as zoonotic disease vectors
Authors: Nangoy Meis , Sondakh Erwin , Koneri Roni , Hadi Upik Kesumawati 
Published: March 2022

Abstract. Nangoy M, Sondakh E, Koneri R, Hadi UK.2021. Fly species on cows around the Tangkoko Nature Reserve, North Sulawesi, Indonesia and their role as disease vectors. Biodiversitas 23: 631-636. Climate and land-use changes in additional to livestock rearing systems play a role in the distribution, species, and population of fly pests, which may pose a risk to humans and animal health. The rearing and grazing of cows at the forest edge and in coconut plantations provide an ideal medium for the growth and development of flies. This study aimed to identify the diversity and abundance of fly species found on cows grazing in two habitat types: forest edge habitat and coconut plantation,located at the Tangkoko Nature Reserve in North Sulawesi, Indonesia. The study was conducted from April 2021 to June 2021 using 24 Ongole Crossbred cows including three adult males and three adult females (250-300 kg) and three males and three female calves (60-75 kg) in each habitat. Flies were collected using two fly nets that were swung along the cows in each habitat type with ten swings. The flies collected were identified based on morphology. A total of 6,982 flies were collected and identified to the following taxonomic groups: Muscidae family (Haematobia exigua 91.21%, Stomoxys calcitrans 1.65%, Musca crassirostris 0.67%, S. indicus 0.54%, S. sitiens 0.42%, and Musca domestica 0.11%), and Tabanidae family (Tabanus factiosus 4.91%, Tabanus striatus 0.47%, and Chrisops sp. 0.01%). The abundance of Haematobia exigua in the coconut plantation habitat was higher than in the forest edges, while the number of T. factiosus at the forest edges was higher than in the coconut plantations. Our results confirm that the presence of a variety of fly species and their abundance depends part on the grazing habitat of the cows. Further study of fly species diversity and pathogen status is critical to provide epidemiological data needed to develop control strategies to prevent the emergence of zoonotic diseases.
Effects of plant growth regulator from seaweed extracts on expression of genes regulating the oil and related metabolites productions in oil palm
Authors: KRESNAWATY IRMA , SANTOSO DJOKO , HUDIYONO SUMI 
Published: March 2022

Abstract. Kresnawaty I, Santoso D,Hudiyono S. 2022. Effects of plant growth regulator from seaweed extracts on expression of genes regulating the oil and related metabolites productions in oil palm. Biodiversitas 23: 793-800. Plant growth regulators (PGRs) have been used in agricultural practicesto regulate various processes, such as germination, vegetative growth, reproduction, maturation, leaf senescence and post-harvest. Seaweed extract as natural PGRs source containsauxin, cytokines, and gibberellins. Although PGRs have been used for decades, the effects of these compounds on the production of secondary metabolites, proteins and related gene-expressionare not yetwell knownin some perennial crops.To study those,treatments consist of trunk injection of oil palm trees with biostimulant that contain PGRs from seaweed extract. Effects of treatments compared were biostimulant (B), biostimulant with activator for oil accumulation (BA) and control (C). The results indicated that the biostimulant treatments affected ACCase Biotin Carboxylase and HMG-CoA genes expression ratio andwere maximum at4 weeks after treatments (WAT) for biostimulant with activators treatments and 8 WAT for biostimulants without activator. Effect of biostimulants treatments in inducing the gene expression was not significant after 8 WAT. Moreover samples with addition biostimulant in 1 and 7 had the highest values ??ofthe gibberellin (GID) gene expression. Oil accumulation showed increase in 4 WAT dan had optimum level in 8 WATbecause of activators addition, especially increased of palmitic and linoleic acid.
Morphological characteristics of local rice in Deli Serdang District, North Sumatra, Indonesia
Authors: CHANIAGO NOVERINA , SULIANSYAH IRFAN , CHANIAGO IRAWATI , ROZEN NALWIDA 
Published: March 2022

Abstract. Chaniago N, Suliansyah I, Chaniago I, Rozen N. 2021. Morphological characteristics of local rice in Deli Serdang District, North Sumatra, Indonesia. Biodiversitas 23: 883-894. The preservation of local rice is essential to preventing genetic drain. In Deli Serdang District, North Sumatra, Indonesia, local rice is in great abundance. A total of 23 Deli Serdang local rice were collected and studied from coastal to highland areas. This study aims to determine the morphological characteristics of 23 local rice genotypes of Deli Serdang and to obtain superior characters to be developed into superior local varieties. A completely randomized design with three replicates was used in this study. Morphological characteristics of 23 local rice genotypes obtained various characters. The culm length was short to very long; culm diameter was thin to thick; the leaf-blade length was long to very long; leaf-blade width was intermediate to broad; culm number was low to high. The auricle, ligule, and collar were almost completely whitish, except for Sijambi yellowish-green and green, 2-cleft acute ligule shape. The harvest age was medium to deep. The grains were white, straw, gold, brown, brown spots, brown lines, and black. The pericarps were white, light brown, red, and black. Most of the organs indicated broad genetic variability having the opportunity to be developed into superior local varieties. All local rice was scattered into five groups based on morphological markers with a similarity of 32.12%.
Phytochemical and antioxidant properties of Syzygium zollingerianum leaves extract
Authors: SUJANA PUTU KRISNA WIDYANTARA , WIJAYANTI NASTITI 
Published: March 2022

Abstract. Sujana PKW, Wijayanti N. 2022. Phytochemical and antioxidant properties of Syzygium zollingerianum leaves extract. Biodiversitas 23: 916-921. The genus Syzygiumis known as a medicinal plant that has broad bioactive properties due to the abundance of bioactive compounds. Syzygium zollingerianum (Miq.) Amshoff could be considered as one of the important species to study because recent reports indicate that this species only grows in Indonesia and studies on its health benefits are still very limited. This study aimed to determine the phytochemical content of the ethanolic extract of S. zollingeranumleaves and to evaluate its antioxidant activity and cytotoxic effects on Vero cell lines. Phytochemical content was analyzed using spectrophotometry, DPPH assay was used to determine antioxidant activity and MTT assay was carried out to determine its cytotoxicity. The results of the spectrophotometric test showed the presence of phenolic, flavonoids, alkaloids, and tannins in the ethanolic extract of S. zollingeranum leaves. The ability to inhibit free radicals was very strong with antioxidant activity index (AAI) of 68.75 ± 31.386 and IC50 of 0.57 ± 0.211 µg/mL. The results of cytotoxicity examination showed that S. zollingerianumleaves extract has moderate cytotoxicity against Vero cells (101.42 ± 6.823 µg/mL). This study showed that S. zollingerianum leaves extract has great potential as a source of natural exogenous antioxidants and conservation for the sustainability of this plant needs to be considered.
Diversity and status of day butterflies (Lepidoptera: Rhopalocera) in different plant associations of the Edough Forest Massif (Northeastern Algeria)
Authors: LAREF NARIMAN , REZZAG-BEDIDA RANIA , BOUKHEROUFA MEHDI , SAKRAOUI RYM , HENADA RANIA LINA IKRAM , HADIBY RACHAD , SAKRAOUI FERIEL 
Published: March 2022

Abstract. Laref N, Rezzag-Beddida R, Boukheroufa M, Sakraoui R, Henada RLI, Hadiby R, Sakraoui F. 2021. Diversity and status of day butterflies (Lepidoptera: Rhopalocera) in different plant associations of the Edough Forest Massif (Northeastern Algeria). Biodiversitas 23: 954-961. The current study seeks to evaluate the biodiversity and the structure of the Lepidoptera, Rhopalocera stand in the Edough mountain range, Northeastern Algeria. The main objective is to analyse specific and dynamic fluctuations depending on the forest species and assess their functional role. The resulting work was conducted during the 2021 spring season at the Ain Bocal Natural Site, characterized by a succession of four forest species in a sampling corridor approximately 2 km long. The sampling strategy was based on the linear transect method derived from the British BMS Butterflies Monitoring Scheme method, where 390 individuals were counted to calculate and analyse the stand structure parameters. We were also able to carry out the taxonomic identification of 13 species belonging to four families, Pieridae, Nymphalidae, Lycaenidae and Papilionidae. Overall, the results revealed that the Mixed Algerian Oak Forest (Zean Oak Forest) is the richest in species, followed by Cork Oak Forest, Algerian Oak Forest and Maritime Pine Forest. The forest of the Edough, by its composition in habitats and ecological niches, allows the installation of the lepidopterological biodiversity.
Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, Indonesia for antibacterial potential against multidrug-resistant bacteria
Authors: PRASTIYANTO MUHAMMAD EVY , KARTIKA APRILIA INDRA , DARMAWATI SRI , RADJASA OCKY KARNA 
Published: March 2022

Abstract. Prastiyanto ME, Kartika AI, Darmawati S, Radjasa OK. 2022. Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, East Nusa Tenggara, Indonesia for antibacterial potential against multidrug-resistant bacteria. Biodiversitas 23: 1118-1124. Marine sponge Spongia sp. has been reported to have potential as an antibacterial agent. However, less information on the potential of bacterial symbionts of Spongia sp. as an antibacterial agent has been documented. The present investigation involves isolating bacterial symbionts of sponge Spongia officinalis and the characterization of antibacterial potential against multidrug-resistant bacteria isolated from clinical specimens. Spongia officinalis was collected from Savu Sea, East Nusa Tenggara, Indonesia and its symbionts were isolated with Zobell marine agar media. The overlay method was used to screen the antibacterial activity against selected six MDR bacteria. Antibacterial activity was determined by measuring the diameter of the inhibition zone. Identification of active bacterial symbionts was carried out based on the 16S rRNA gene sequencing. The results revealed that four out of 10 symbionts showed antibacterial activity against MDR bacteria with an inhibition index ranging from 4.8 to 12.6 mm. Prastiyanto-1A and Prastiyanto-1E isolates demonstrated antibacterial activity against ESBL- Escherichia coli and ESBL + CRE- Klebsiella pneumoniae subsp. pneumoniae, Pratiyanto-2A isolate showed antibacterial activity against MRSA, while Prastiyanto-4A isolates showed antibacterial activity against CRPA. The selected four isolates were identified as Bacillus subtilis, Bacillus mojavensis and Bacillus simplex using 16S rRNA gene sequencing and BLASTn analysis. These results provide information about the potential of bacterial symbionts of S. officinalis as natural antibacterial sources against MDR bacteria.
Antibacterial activity of bacteria isolated from earthworm (Pheretima sp.) gut against Salmonella typhi and Staphylococcus aureus: in vitro experiments supported by computational docking
Authors: HUSAIN DIRAYAH RAUF , WARDHANI RIUH , ERVIANI ANDI EVI 
Published: March 2022

Abstract. Husain DR, Wardhani R, Erviani AE. 2022. Antibacterial activity of bacteria isolated from earthworm (Pheretimasp.) gut against Salmonella typhiand Staphylococcus aureus: in vitro experiments supported by computational docking. Biodiversitas 23: 1125-1131. Bacteria have a diverse ecology niche as the effect of a long evolutionary process. They can live along with other organisms as endosymbiont. Research into endosymbiont bacteria began because of their ability to increase host resistance, especially to pathogens. This study aimed to determine the antimicrobial ability of endosymbiont bacterial isolates of earthworms Pheretimasp., Bacillus brevis, and Bacillus choshinensis that inhabit Pheretimasp. were isolated.The isolates were grown on tryptic soy broth media for 24 hours. The isolates were then purified using tryptic soy agar and biochemically tested to ensure both isolates are endosymbiotic bacteria. Antimicrobial activity was tested using agar diffusion methods in pathogenic bacteria, Staphylococcus aureus, and Salmonella typhi. Amoxicillin and chloramphenicol were used as positive controls. The inhibitory test results showed that incubation for 15 days effectively assessed pathogenic bacteria growth inhibition, marked by the largest inhibition zone (21.32 mm for Salmonella typhi and 16.88 mm for Staphylococcus aureus). They were very effective at inhibiting Salmonella typhiand Staphylococcus aureus growths.Endosymbiotic compounds’ had a potential as antimicrobial. The in silico test supports the inhibitory test, which concluded that endosymbiont isolates can be an antimicrobial characterized by low-binding affinity values (-8,6 on tyrocidine) on molecular docking analysis.
Seroepidemiology of SARS-CoV-2 in healthcare personnel working at the largest tertiary COVID-19 referral hospitals in Mexico City
Authors: Dávila-Conn Vanessa , Soto-Nava Maribel , Caro-Vega Yanink N. , Paz-Juárez Héctor E. , García-Esparza Pedro , Tapia-Trejo Daniela , Pérez-García Marissa , Belaunzarán-Zamudio Pablo F. , Reyes-Terán Gustavo , Sierra-Madero Juan G. , Galindo-Fraga Arturo , Ávila-Ríos Santiago 
Published: March 2022

Introduction
We performed a longitudinal SARS-CoV-2 seroepidemiological study in healthcare personnel of the two largest tertiary COVID-19 referral hospitals in Mexico City.

Methods
All healthcare personnel, including staff physicians, physicians in training, nurses, laboratory technicians, researchers, students, housekeeping, maintenance, security, and administrative staff were invited to voluntarily participate, after written informed consent. Participants answered a computer-assisted self-administered interview and donated blood samples for antibody testing every three weeks from October 2020 to June 2021.

Results
A total of 883 participants (out of 3639 registered employees) contributed with at least one blood sample. The median age was 36 years (interquartile range: 28–46) and 70% were women. The most common occupations were nurse (28%), physician (24%), and administrative staff (22%). Two hundred and ninety participants (32.8%) had a positive-test result in any of the visits, yielding an overall adjusted prevalence of 33.5% for the whole study-period. Two hundred and thirty-five positive tests were identified at the baseline visit (prevalent cases), the remaining 55 positive tests were incident cases. Prevalent cases showed associations with both occupational (institution 2 vs. 1: adjusted odds ratio [aOR] = 2.24, 95% confidence interval [CI]: 1.54–3.25; laboratory technician vs. physician: aOR = 4.38, 95% CI: 1.75–10.93) and community (municipality of residence Xochimilco vs. Tlalpan: aOR = 2.03, 95% CI: 1.09–3.79) risk-factors. The incidence rate was 3.0 cases per 100 person-months. Incident cases were associated with community-acquired risk, due to contact with suspect/confirmed COVID-19 cases (HR = 2.45, 95% CI: 1.21–5.00).

Conclusions
We observed that between October 2020 and June 2021, healthcare workers of the two largest tertiary COVID-19 referral centers in Mexico City had similar level of exposure to SARS-CoV-2 than the general population. Most variables associated with exposure in this setting pointed toward community rather than occupational risk. Our observations are consistent with successful occupational medicine programs for SARS-CoV-2 infection control in the participating institutions but suggest the need to strengthen mitigation strategies in the community.
Genotyping of familial Mediterranean fever gene (MEFV)—Single nucleotide polymorphism—Comparison of Nanopore with conventional Sanger sequencing
Authors: Schmidt Jonas , Berghaus Sandro , Blessing Frithjof , Herbeck Holger , Blessing Josef , Schierack Peter , Rödiger Stefan , Roggenbuck Dirk , Wenzel Folker 
Published: March 2022

Background
Through continuous innovation and improvement, Nanopore sequencing has become a powerful technology. Because of its fast processing time, low cost, and ability to generate long reads, this sequencing technique would be particularly suitable for clinical diagnostics. However, its raw data accuracy is inferior in contrast to other sequencing technologies. This constraint still results in limited use of Nanopore sequencing in the field of clinical diagnostics and requires further validation and IVD certification.

Methods
We evaluated the performance of latest Nanopore sequencing in combination with a dedicated data-analysis pipeline for single nucleotide polymorphism (SNP) genotyping of the familial Mediterranean fever gene (MEFV) by amplicon sequencing of 47 clinical samples. Mutations in MEFV are associated with Mediterranean fever, a hereditary periodic fever syndrome. Conventional Sanger sequencing, which is commonly applied in clinical genetic diagnostics, was used as a reference method.

Results
Nanopore sequencing enabled the sequencing of 10 target regions within MEFV with high read depth (median read depth 7565x) in all samples and identified a total of 435 SNPs in the whole sample collective, of which 29 were unique. Comparison of both sequencing workflows showed a near perfect agreement with no false negative calls. Precision, Recall, and F1-Score of the Nanopore sequencing workflow were > 0.99, respectively.

Conclusions
These results demonstrated the great potential of current Nanopore sequencing for application in clinical diagnostics, at least for SNP genotyping by amplicon sequencing. Other more complex applications, especially structural variant identification, require further in-depth clinical validation.