bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hearing Epistemic Sound in Experimental (Music) Systems, 1958–73
Authors: Andersen Drake 
Published: October 2022

AbstractFrom the late 1950s to the mid-1970s, American experimental musicians like Pauline Oliveros, David Tudor, and Gordon Mumma employed complex and idiosyncratic technological systems to produce and capture acoustic resonance for aesthetic appreciation. Although this shared exploration exhibited many of the hallmarks of a genuine research project, scholars of experimental music have long been wary of claims that there is anything particularly scientific about this music, frequently comparing its informality unfavorably with the rigor and empiricism of the individual scientific experiment. However, historian of science Hans-Jörg Rheinberger has long held that the fundamental working unit of scientific research is not the individual experiment, but what he terms the experimental system: The loose coherence of objects, instruments, and technologies through which research questions are materialized over time. I argue that Rheinberger's framework of the experimental system offers a compelling way of understanding the experimentation that catalyzed the emergence of what has come to be known as “resonance aesthetics” in American experimental music. By focusing on the material links of musicians’ activities, the experimental system illuminates how knowledge was produced and circulated within and between vastly different musical performances. Rheinberger's characterization of successful research also informs a more nuanced conception of virtuosity in experimental music. Finally, this framework is an opportunity to re-evaluate the status of sound as an object of epistemological inquiry, akin to what Rheinberger describes as an “epistemic thing.” In theorizing epistemic sound as both contextual and emergent, I re-evaluate musicians’ approaches to spontaneity and improvisation in musical performance.
Impact of dielectric substrates on chipless RFID tag performance
Authors: Ali Amjad , Smartt Christopher , Im Jisun , Williams Orla , Lester Ed , Greedy Steve 
Published: October 2022

AbstractA five-slot hexagonal shape chipless RFID tag is designed, simulated, and manufactured on FR4 substrate. The designed tag's copper geometry was replicated on a wide range of dielectric substrate to quantify the impact on resonance quality factor (RQF) and resonating frequencies. The tag's performance was assessed in three configurations. First, a hexagonal shape tag's radar cross section (RCS) was studied over different dielectric substrates. The various dielectric substrate effects were investigated over the maximum read range, resonant frequencies and RQF. In the second evaluation, the physical geometry of the tag was adjusted to achieve the spectral signatures in 2–7 GHz frequency band with high RQF. In step three, the optimized tag geometry was manufactured on FR4, Roger Duroid 5880, and polyethylene naphthalate (PEN) substrates. Denford milling machine for PCB engraving and inkjet printing for silver nanoparticles deposition were used for tags manufacturing. During tag manufacturing, copper and silver were used as conducting materials for RCS backscattering. The tag RCS response was measured by vector network analyzer with bi-static antenna setup. The analysis of different dielectric substrate provides a pathway of designing a novel substrate by using various nanomaterials.
Tsunami Early Warning From Global Navigation Satellite System Data Using Convolutional Neural Networks
Authors: Rim Donsub , Baraldi Robert , Liu Christopher M. , LeVeque Randall J. , Terada Kenjiro 
Published: October 2022

AbstractWe investigate the potential of using Global Navigation Satellite System (GNSS) observations to directly forecast full tsunami waveforms in real time. We train convolutional neural networks to use less than 9 min of GNSS data to forecast the full tsunami waveforms over 6 hr at select locations, and obtain accurate forecasts on a test data set. Our training and test data consists of synthetic earthquakes and associated GNSS data generated for the Cascadia Subduction Zone using the MudPy software, and corresponding tsunami waveforms in Puget Sound computed using GeoClaw. We use the same suite of synthetic earthquakes and waveforms as in earlier work where tsunami waveforms were used for forecasting, and provide a comparison. We also explore varying the number of GNSS stations, their locations, and their observation durations.
Thermocline Salinity Minima Due To Wind‐Driven Differential Advection
Authors: Chrysagi Evridiki , Basdurak N. Berkay , Umlauf Lars , Gräwe Ulf , Burchard Hans 
Published: October 2022

AbstractObservations from the global ocean have long confirmed the ubiquity of thermohaline inversions in the upper ocean, often accompanied by a clear signal in biogeochemical properties. Their emergence has been linked to different processes such as double diffusion, mesoscale stirring, frontal subduction, and the recently discussed submesoscale features. This study uses the central Baltic Sea as a natural laboratory to explore the formation of salinity inversions in the thermocline region during summer. We use realistic high‐resolution simulations complemented by field observations to identify the dominant generation mechanism and potential hotspots of their emergence. We propose that the strongly stratified thermocline can host distinct salinity minima during summer conditions resulting primarily from the interaction between lateral surface salinity gradients and wind‐induced differential advection. Since this is a generic mechanism, such salinity inversions can likely constitute a typical feature of the upper ocean in regions with distinct thermoclines and shallow mixed layers.
Cntnap2-dependent molecular networks in autism spectrum disorder revealed through an integrative multi-omics analysis
Authors: Jang Wooyoung Eric , Park Ji Hwan , Park Gaeun , Bang Geul , Na Chan Hyun , Kim Jin Young , Kim Kwang-Youl , Kim Kwang Pyo , Shin Chan Young , An Joon-Yong , Lee Yong-Seok , Kim Min-Sik 
Published: October 2022

AbstractAutism spectrum disorder (ASD) is a major neurodevelopmental disorder in which patients present with core symptoms of social communication impairment, restricted interest, and repetitive behaviors. Although various studies have been performed to identify ASD-related mechanisms, ASD pathology is still poorly understood. CNTNAP2 genetic variants have been found that represent ASD genetic risk factors, and disruption of Cntnap2 expression has been associated with ASD phenotypes in mice. In this study, we performed an integrative multi-omics analysis by combining quantitative proteometabolomic data obtained with Cntnap2 knockout (KO) mice with multi-omics data obtained from ASD patients and forebrain organoids to elucidate Cntnap2-dependent molecular networks in ASD. To this end, a mass spectrometry-based proteometabolomic analysis of the medial prefrontal cortex in Cntnap2 KO mice led to the identification of Cntnap2-associated molecular features, and these features were assessed in combination with multi-omics data obtained on the prefrontal cortex in ASD patients to identify bona fide ASD cellular processes. Furthermore, a reanalysis of single-cell RNA sequencing data obtained from forebrain organoids derived from patients with CNTNAP2-associated ASD revealed that the aforementioned identified ASD processes were mainly linked to excitatory neurons. On the basis of these data, we constructed Cntnap2-associated ASD network models showing mitochondrial dysfunction, axonal impairment, and synaptic activity. Our results may shed light on the Cntnap2-dependent molecular networks in ASD.
Antidepressants for the treatment of adults with major depressive disorder in the maintenance phase: a systematic review and network meta-analysis
Authors: Kishi Taro , Ikuta Toshikazu , Sakuma Kenji , Okuya Makoto , Hatano Masakazu , Matsuda Yuki , Iwata Nakao 
Published: October 2022

AbstractA systematic review and random-effects model network meta-analysis were conducted to compare the efficacy, acceptability, tolerability, and safety of antidepressants to treat adults with major depressive disorder (MDD) in the maintenance phase. This study searched the PubMed, Cochrane Library, and Embase databases and included only double-blind, randomized, placebo-controlled trials with an enrichment design: patients were stabilized on the antidepressant of interest during the open-label study and then randomized to receive the same antidepressant or placebo. The outcomes were the 6-month relapse rate (primary outcome, efficacy), all-cause discontinuation (acceptability), discontinuation due to adverse events (tolerability), and the incidence of individual adverse events. The risk ratio with a 95% credible interval was calculated. The meta-analysis comprised 34 studies (n = 9384, mean age = 43.80 years, and %females = 68.10%) on 20 antidepressants (agomelatine, amitriptyline, bupropion, citalopram, desvenlafaxine, duloxetine, escitalopram, fluoxetine, fluvoxamine, levomilnacipran, milnacipran, mirtazapine, nefazodone, paroxetine, reboxetine, sertraline, tianeptine, venlafaxine, vilazodone, and vortioxetine) and a placebo. In terms of the 6-month relapse rate, amitriptyline, citalopram, desvenlafaxine, duloxetine, fluoxetine, fluvoxamine, mirtazapine, nefazodone, paroxetine, reboxetine, sertraline, tianeptine, venlafaxine, and vortioxetine outperformed placebo. Compared to placebo, desvenlafaxine, paroxetine, sertraline, venlafaxine, and vortioxetine had lower all-cause discontinuation; however, sertraline had a higher discontinuation rate due to adverse events. Compared to placebo, venlafaxine was associated with a lower incidence of dizziness, while desvenlafaxine, sertraline, and vortioxetine were associated with a higher incidence of nausea/vomiting. In conclusion, desvenlafaxine, paroxetine, venlafaxine, and vortioxetine had reasonable efficacy, acceptability, and tolerability in the treatment of adults with stable MDD.
Genome-wide association study meta-analysis of suicide death and suicidal behavior
Authors: Li Qingqin S. , Shabalin Andrey A. , DiBlasi Emily , Gopal Srihari , Canuso Carla M. ,   FinnGen, International Suicide Genetics Consortium, Palotie Aarno , Drevets Wayne C. , Docherty Anna R. , Coon Hilary 
Published: October 2022

AbstractSuicide is a worldwide health crisis. We aimed to identify genetic risk variants associated with suicide death and suicidal behavior. Meta-analysis for suicide death was performed using 3765 cases from Utah and matching 6572 controls of European ancestry. Meta-analysis for suicidal behavior using data across five cohorts (n = 8315 cases and 256,478 psychiatric or populational controls of European ancestry) was also performed. One locus in neuroligin 1 (NLGN1) passing the genome-wide significance threshold for suicide death was identified (top SNP rs73182688, withp = 5.48 × 10−8before andp = 4.55 × 10−8after mtCOJO analysis conditioning on MDD to remove genetic effects on suicide mediated by MDD). Conditioning on suicidal attempts did not significantly change the association strength (p = 6.02 × 10−8), suggesting suicide death specificity.NLGN1encodes a member of a family of neuronal cell surface proteins. Members of this family act as splice site-specific ligands for beta-neurexins and may be involved in synaptogenesis. The NRXN-NLGN pathway was previously implicated in suicide, autism, and schizophrenia. We additionally identifiedROBO2andZNF28associations with suicidal behavior in the meta-analysis across five cohorts in gene-based association analysis using MAGMA. Lastly, we replicated two loci including variants nearSOX5andLOC101928519associated with suicidal attempts identified in the ISGC and MVP meta-analysis using the independent FinnGen samples. Suicide death and suicidal behavior showed positive genetic correlations with depression, schizophrenia, pain, and suicidal attempt, and negative genetic correlation with educational attainment. These correlations remained significant after conditioning on depression, suggesting pleiotropic effects among these traits. Bidirectional generalized summary-data-based Mendelian randomization analysis suggests that genetic risk for the suicidal attempt and suicide death are both bi-directionally causal for MDD.
A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer
Authors: Xu Tiantian , Ma Qisheng , Li Yanan , Yu Qing , Pan Peichen , Zheng Yawen , Li Zhijian , Xiong Xiufang , Hou Tingjun , Yu Bin , Liu Hongmin , Sun Yi 
Published: October 2022

AbstractProtein neddylation is catalyzed by a neddylation activating enzyme (NAE, E1), an E2 conjugating enzyme, and an E3 ligase. In various types of human cancers, the neddylation pathway is abnormally activated. Our previous study validated that the neddylation E2 UBE2F is a promising therapeutic target in lung cancer. Although the NAE inhibitor MLN4924/pevonedistat is currently under clinical investigation as an anti-cancer agent, there are no small molecules available that selectively target UBE2F. Here, we report, for the first time, the discovery, via structure-based virtual screen and chemical optimization, of such a small molecule, designated as HA-9104. HA-9104 binds to UBE2F, reduces its protein levels, and consequently inhibits cullin-5 neddylation. Blockage of cullin-5 neddylation inactivates cullin-RING ligase-5 (CRL5) activity, leading to accumulation of the CRL5 substrate, NOXA, to induce apoptosis. Moreover, HA-9104 appears to form the DNA adduct via its 7-azaindole group to induce DNA damage and G2/M arrest. Biologically, HA-9104 effectively suppresses the growth and survival of lung cancer cells and confers radiosensitization in both in vitro cell culture and in vivo xenograft tumor models. In summary, we discovered a small molecule, designated HA-9104, that targets the UBE2F-CRL5 axis with anti-cancer activity alone or in combination with radiation.
Bone marrow mesenchymal stem cell-derived exosomal miR-21a-5p alleviates renal fibrosis by attenuating glycolysis by targeting PFKM
Authors: Xu Shihao , Cheuk Yin Celeste , Jia Yichen , Chen Tian , Chen Juntao , Luo Yongsheng , Cao Yirui , Guo Jingjing , Dong Lijun , Zhang Yi , Shi Yi , Rong Ruiming 
Published: October 2022

AbstractRenal fibrosis is a common pathological feature and outcome of almost all chronic kidney diseases, and it is characterized by metabolic reprogramming toward aerobic glycolysis. Mesenchymal stem cell-derived exosomes (MSC-Exos) have been proposed as a promising therapeutic approach for renal fibrosis. In this study, we investigated the effect of MSC-Exos on glycolysis and the underlying mechanisms. We demonstrated that MSC-Exos significantly ameliorated unilateral ureter obstruction (UUO)-induced renal fibrosis by inhibiting glycolysis in tubular epithelial cells (TECs). miRNA sequencing showed that miR-21a-5p was highly enriched in MSC-Exos. Mechanistically, miR-21a-5p repressed the expression of phosphofructokinase muscle isoform (PFKM), a rate-limiting enzyme of glycolysis, thereby attenuating glycolysis in TECs. Additionally, knockdown of miR-21a-5p abolished the renoprotective effect of MSC-Exos. These findings revealed a novel role for MSC-Exos in the suppression of glycolysis, providing a new insight into the treatment of renal fibrosis.
PDCL2 is essential for spermiogenesis and male fertility in mice
Authors: Li Minyan , Chen Yuxi , Ou Jianping , Huang Junjiu , Zhang Xiya 
Published: October 2022

AbstractPatients with teratozoospermia exhibit low phosducin-like protein (Pdcl2) expression. As a member of the phosducin family, chaperonin-related Pdcl2, a germline-specific gene, may be involved in germ cell protein folding. Given that PDCL2 is highly conserved in evolution, it may be indispensable for mammalian spermiogenesis; however, the function of PDCL2 in higher mammalian species remains unknown. To determine the role of PDCL2 in male fertility, we generated Pdcl2 knockout mice using CRISPR/Cas9. Our results revealed that Pdcl2 heterozygous (Pdcl2+/−) male mice were normal, but male Pdcl2-null (Pdcl2−/−) mice were infertile. Accordingly, Pdcl2−/− male mice exhibited lower testis weight, epididymis weight, and sperm number than Pdcl2+/+ mice. Moreover, Pdcl2−/− mice displayed malformed and immotile sperm. Apoptotic cells were significantly enhanced in Pdcl2−/− testes and epididymis when compared with those in wild-type mice. Mechanistically, PDCL2 can interact with the CCT complex, and dysfunction in this complex might lead to infertility in Pdcl2−/− male mice. Collectively, these findings confirm that Pdcl2 knockout leads to male infertility in mice and that PDCL2 may function as a chaperone to promote protein folding during spermiogenesis.
Hydrodynamic conditions affect the proteomic profile of marine biofilms formed by filamentous cyanobacterium
Authors: Romeu Maria J. , Domínguez-Pérez Dany , Almeida Daniela , Morais João , Araújo Mário J. , Osório Hugo , Campos Alexandre , Vasconcelos Vítor , Mergulhão Filipe J. 
Published: October 2022

AbstractProteomic studies on cyanobacterial biofilms can be an effective approach to unravel metabolic pathways involved in biofilm formation and, consequently, obtain more efficient biofouling control strategies. Biofilm development by the filamentous cyanobacterium Toxifilum sp. LEGE 06021 was evaluated on different surfaces, glass and perspex, and at two significant shear rates for marine environments (4 s−1 and 40 s−1). Higher biofilm development was observed at 4 s−1. Overall, about 1877 proteins were identified, and differences in proteome were more noticeable between hydrodynamic conditions than those found between surfaces. Twenty Differentially Expressed Proteins (DEPs) were found between 4 s−1 vs. 40 s−1. On glass, some of these DEPs include phage tail proteins, a carotenoid protein, cyanophynase glutathione-dependent formaldehyde dehydrogenase, and the MoaD/ThiS family protein, while on perspex, DEPs include transketolase, dihydroxy-acid dehydratase, iron ABC transporter substrate-binding protein and protein NusG. This study contributes to developing a standardized protocol for proteomic analysis of filamentous cyanobacterial biofilms. This kind of proteomic analysis can also be useful for different research fields, given the broad spectrum of promising secondary metabolites and added-value compounds produced by cyanobacteria, as well as for the development of new antibiofilm strategies.
Chromosomal inversion polymorphisms shape the genomic landscape of deer mice
Authors: Harringmeyer Olivia S. , Hoekstra Hopi E. 
Published: October 2022

AbstractChromosomal inversions are an important form of structural variation that can affect recombination, chromosome structure and fitness. However, because inversions can be challenging to detect, the prevalence and hence the significance of inversions segregating within species remains largely unknown, especially in natural populations of mammals. Here, by combining population-genomic and long-read sequencing analyses in a single, widespread species of deer mouse (Peromyscus maniculatus), we identified 21 polymorphic inversions that are large (1.5–43.8 Mb) and cause near-complete suppression of recombination when heterozygous (0–0.03 cM Mb−1). We found that inversion breakpoints frequently occur in centromeric and telomeric regions and are often flanked by long inverted repeats (0.5–50 kb), suggesting that they probably arose via ectopic recombination. By genotyping inversions in populations across the species’ range, we found that the inversions are often widespread and do not harbour deleterious mutational loads, and many are likely to be maintained as polymorphisms by divergent selection. Comparisons of forest and prairie ecotypes of deer mice revealed 13 inversions that contribute to differentiation between populations, of which five exhibit significant associations with traits implicated in local adaptation. Taken together, these results show that inversion polymorphisms have a significant impact on recombination, genome structure and genetic diversity in deer mice and likely facilitate local adaptation across the widespread range of this species.
Materials analysis and image-based modelling of transmissibility and strain behaviour in approved face mask microstructures
Authors: Rasekh Manoochehr , Pisapia Francesca , Howkins Ashley , Rees David 
Published: October 2022

AbstractComparisons are made between six different approved face masks concerning their particle transmissibility allied to mechanical properties. The latter involves material testing and stretch or strain behaviour under load. SEM and X-ray elemental analyses showed contrasting structures between random and ordered fibre orientations. These constitute the mask designs where transmissibility is to be minimised. Airflow velocity measurement enabled filtration to be measured between the different mask designs, from two to six layers of different fabrics in combination. SEM provided the fibre diameter and pore size of each mask layer, up to a maximum of six. Stretching each complete mask showed its elasticity and recovery behaviour on an energy basis. The energy conversion involved in mask straining involves areas enclosed within steady and cyclic load-extension plots. Thus, the work done in extending a mask and the energy recovered from its release identified a hysteresis associated with an irrecoverable permanent stretch to the mask fabric. Failure of individual layers, which occurred successively in extended stretch tests, appeared as a drop in a load-extension response. That change is associated with permanent damage to each mask and friction contact within the rearrangement of loose fibre weaves. Masks with the greatest number of layers reduced particle transmissibility. However, woven or ordered mask fabrics in two layers with different orientations provided comparable performance. Simulation of each mechanical response, velocity streamlining and fibre distribution within the mask layers are also presented.
Narrow-band power dividers with wide range tunable power-dividing ratio
Authors: Zarghami Sepehr , Hayati Mohsen 
Published: October 2022

AbstractThis paper presents two narrow-band power dividers with a wide range power-dividing ratio based on the two new controlling insertion loss methods, which are low-impedance line and coupling capacitor. Initially, a narrow-band BPF is designed based on the equivalent circuit model and LC equivalent circuit. Then, using the surface current density, it is determined by which part of BPF structure the insertion loss (IL) can be controlled at center frequency. The tunable Wilkinson power dividers (TWPDs) are designed based on IL control components to create a wide range of power-dividing ratios, using only two DC voltages. The center frequency of first designed TWPD is 2.5 GHz, and the power-dividing ratio can be controlled up to 1:45 by variation of two DC voltages from 0 to 8 V. Since the structure of TWPDs are symmetric, the inverse voltages results in the inverted divided power between the output ports. The center frequency of second designed TWPD is 2.52 GHz, and power-dividing ratio can be controlled up to 1:134 by variation of two DC voltages from 1.7 to 4 V. Two proposed TWPDs are fabricated and measured. Comparisons of the measured and simulated results are presented to verify the theoretical predictions.