bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rehabilitation Including Structured Active Play for Preschoolers With Cancer (RePlay)—Study Protocol for a Randomized Controlled Trial
Authors: Pouplier Anna , Winther Helle , Christensen Jan , Schmidt-Andersen Peter , Zhang He , Frandsen Thomas Leth , Schmiegelow Kjeld , Fridh Martin Kaj , Larsen Hanne Bækgaard 
Published: May 2022

BackgroundChildren diagnosed with cancer experience muscle weakness and impaired physical function caused by treatment and related immobility. The situation forces them into a negative cycle of diminished participation in physical and leisure activities and isolation from peers; inhibiting the natural development of social and gross motor skills. This manuscript presents a protocol for a study that explores the effects of using structured active play to maintain preschoolers' age specific gross motor function and social and personal skills while undertaking intensive cancer treatment.MethodsThe study is a two-arm, superiority randomized controlled trial with an intervention and a control group designed to evaluate the effects of a structured active play intervention on gross motor function. Gross motor subtests of the Peabody Developmental Motor Scales, Second Edition (PDMS-2) are used for measurement; with the primary end-point at 6 months post-treatment initiation. Eighty-four preschool children (aged 1–5 years), newly diagnosed with cancer at the Copenhagen University Hospital are randomly assigned to either an intervention or control group, using a 1:1 allocation. The intervention group receives a combined in-hospital and home-based program that includes structured active play activities, while the control group receives standard care, including physiotherapy. During hospital admission, the intervention group undertakes 45-min structured active play group sessions three times weekly, conducted by exercise professionals. Parents receive training and supervision to facilitate daily individual sessions outside of group sessions. Secondary study outcomes target the children's overall function level in everyday life, general physical performance, and health-related quality of life. As well, children's and parents' experiences within the intervention are explored and the children's social and personal development is observed.DiscussionLimited evidence exists regarding the effectiveness of rehabilitation interventions, particularly those including active play, for preschoolers diagnosed with cancer. This manuscript reporting on a study protocol will enhance clarity and transparency in reporting and offer insights for others with interest in this same topic. Once completed, findings from this study could extend knowledge about the conduct and measurement of effectiveness in rehabilitation initiatives. If study findings suggest that the intervention is effective, structured active play may become a standard part of rehabilitation.Trial RegistrationClinicalTrials.gov: NCT04672681. Registered December 17, 2020. https://clinicaltrials.gov/ct2/show/NCT04672681.
Src Activation Aggravates Podocyte Injury in Diabetic Nephropathy via Suppression of FUNDC1-Mediated Mitophagy
Authors: Zheng Ting , Wang Han-yu , Chen Yang , Chen Xiao , Wu Zi-ling , Hu Qin-yu , Sun Hui 
Published: May 2022

Background and purpose: Mitophagy plays a significant role in the progression of diabetic nephropathy (DN), although the regulatory mechanisms remain unclear. Recently, accumulating evidence demonstrated that impaired mitochondrial function and mitophagy are involved in DN. Here, we are aimed to explore the role of c-Src (Src) and FUNDC1-related mitophagy in the development of DN.Methods: The db/db mice were used to establish a DN mice model. The mice accepted PP2 (Src inhibitor) treatment to study the role of Src in DN. Kidney function was measured via biochemical testing. Renal histopathology and morphometric analysis were conducted via hematoxylin-eosin (HE), periodic acid-Schiff (PAS), Masson’s staining, and transmission electron microscopy (TEM). We measured degree of apoptosis in kidney by TUNEL assay. Indices of mitophagy (LC3 and p62) were evaluated by Western blotting and immunofluorescence. Complementary in vitro assays were conducted using human podocytes subjected to high glucose in combination with PP2 treatment or FUNDC1 small interfering RNAs (siRNAs). Flow cytometry was used to detect the apoptotic cells. Mitochondrial function was evaluated by JC-1 staining. Double immunofluorescence labeling of LC3 and TOMM20 used to assess the degree of mitophagy.Results: Increased Src activation was detected in the kidneys of db/db mice, and its expression was positively correlated with mitochondrial damage, podocyte apoptosis, and renal dysfunction. Inhibition of Src activation with PP2 protected against mitochondrial damage and podocyte apoptosis. In vitro experiments in podocytes established that high glucose increased Src activation, promoting FUNDC1 phosphorylation and inhibiting mitophagy. Consistent with the mouse model, inhibiting Src activity protected podocytes against mitochondrial damage. FUNDC1 silencing negated the actions of PP2, indicating that FUNDC1-mediated mitophagy is downstream pathway of Src.Conclusion: In summary, our data indicated that Src is a culprit factor in diabetic renal damage via suppression of FUNDC1-mediated mitophagy, promoting the development of DN.
The Application Value of the Renal Region of Interest Corrected by Computed Tomography in Single-Kidney Glomerular Filtration Rate for the Evaluation of Patients With Moderate or Severe Hydronephrosis
Authors: Yin Haixia , Liang Wenli , Zhao Deshan 
Published: May 2022

Objective: This study aimed to investigate the application value of the renal region of interest (ROI) corrected by computed tomography (CT) in single-kidney glomerular filtration rate (GFR) in patients with hydronephrosis.Methods: A total of 46 patients with hydronephrosis were divided into four groups based on their degree of unilateral hydronephrosis: a normal group (left kidney and right kidney) and three abnormal groups (mild, moderate, and severe hydronephrosis). GFR was measured using the two-sample method (tGFR). The single-kidney GFR of each patient was derived from differential renal function values in dynamic renal imaging multiplied by GFR. The single-kidney GFRs, including GFR from the Gates method (gGFRsingle) and CT area-corrected GFR (aGFRsingle), were compared with tGFRsingle. A paired-sample t-test and Pearson’s test were used for data analysis. p &lt; 0.05 was considered statistically significant.Results: There were no significant differences between aGFRsingle and tGFRsingle in patients in the normal, mild hydronephrosis, and moderate hydronephrosis groups (t = –0.604∼1.982, all p &gt; 0.05), but there was a significant difference between them in the severe hydronephrosis group (t = 2.302, p &lt; 0.05). There were no significant differences between gGFRsingle and tGFRsingle in the normal and mild hydronephrosis groups (t = 0.194∼0.962, all p &gt; 0.05), but there was a significant difference between them in the moderate and severe hydronephrosis groups (t = 3.321, 3.494, p &lt; 0.05). Both gGFRsingle and aGFRsingle were correlated with tGFRsingle, with aGFRsingle being more strongly correlated (r = 0.890, p &lt; 0.001).Conclusion: In patients with moderate hydronephrosis, aGFRsingle is more strongly correlated with tGFRsingle than gGFRsingle. However, in patients with severe hydronephrosis and accompanying renal morphological changes, the aGFRsingle measured by the renal ROI area-correction method using CT has higher accuracy and better clinical application value than the conventional gGFRsingle.
Alterations of Static and Dynamic Functional Connectivity of the Nucleus Accumbens in Patients With Major Depressive Disorder
Authors: Zhou Bingqian , Chen Yuan , Zheng Ruiping , Jiang Yu , Li Shuying , Wei Yarui , Zhang MengZhe , Gao XinYu , Wen Baohong , Han Shaoqiang , Cheng Jingliang 
Published: May 2022

BackgroundMajor depressive disorder (MDD) is associated with dysfunction of the reward system. As an important node in the reward system, the resting-state functional connectivity of the nucleus accumbens (NAc) is related to the etiology of MDD. However, an increasing number of recent studies propose that brain activity is dynamic over time, no study to date has examined whether the NAc dynamic functional connectivity (DFC) is changed in patients with MDD. Moreover, few studies have examined the impact of the clinical characteristics of patients with MDD.MethodsA total of 220 MDD patients and 159 healthy controls (HCs), group-matched for age, sex, and education level, underwent resting-state functional magnetic resonance imagining (rs-fMRI) scans. Seed-based resting-state functional connectivity (RSFC) and DFC of the NAc were conducted. Two sample t-tests were performed to alter RSFC/DFC of NAc. In addition, we examined the association between altered RSFC/DFC and depressive severity using Pearson correlation. Finally, we divided patients with MDD into different subgroups according to clinical characteristics and tested whether there were differences between the subgroups.ResultsCompared with the HCs, MDD patients show reduced the NAc-based RSFC with the dorsolateral prefrontal cortex (DLPFC), hippocampus, middle temporal gyrus (MTG), inferior temporal gyrus (ITG), precuneus, and insula, and patients with MDD show reduced the NAc-based DFC with the DLPFC, ventromedial prefrontal cortex (VMPFC), ventrolateral prefrontal cortex (VLPFC), MTG, ITG, and insula. MDD severity was associated with RSFC between the NAc and precentral gyrus (r = 0.288, p = 0.002, uncorrected) and insula (r = 0.272, p = 0.003, uncorrected).ConclusionThis study demonstrates abnormal RSFC and DFC between the NAc and distributed cerebral regions in MDD patients, characterized by decreased RSFC and DFC of the NAc connecting with the reward, executive, default-mode, and salience network. Our results expand previous descriptions of the NAc RSFC abnormalities in MDD, and the altered RSFC/DFC may reflect the disrupted function of the NAc.
Uncommon Cause of Right Upper Quadrant Pain Treated by Laparoscopic Surgery: A Case Report
Authors: Feng Qingbo , Du Jinpeng , Liao Wenwei , Zheng Jinli , Zeng Yong , Li Jiaxin 
Published: May 2022

IntroductionRight upper quadrant pain is a very common symptom of cholecystitis. Right upper quadrant pain caused by fish bone perforation of the stomach wall is rare.Case PresentationWe report a 42-year-old woman who was admitted to our hospital with “1-month history of dull progressive right upper quadrant pain radiating to the back.” Computed tomography of the abdomen revealed a linear, high-density body between the stomach wall and the liver. On history, the patient stated she had eaten a bony fish a month prior but did not note any significant pain at the time. Laparoscopy revealed a fish bone 2 cm in length half on the surface of the caudate lobe of the liver, and no perforation of the gastrointestinal tract was found. The postoperative course was uncomplicated, and the patient was discharged home on day 3 after surgery.ConclusionThe case of right upper quadrant pain caused by the fish bone is very rare. Radiological examinations play a significant role in the diagnosis of fish bone ingestion. Laparoscopic surgery is technically feasible and safe for the treatment of patients with fish bone ingestion.
ANTOCIANINAS EN UVA (Vitis vinifera L.) Y SU RELACIÓN CON EL COLOR
Authors: Del Valle-Leguizamón Graciela , González-León Alberto , Báez-Sañudo Reginaldo 
Published: May 2022

Las antocianinas son compuestos fenólicos que se encuentran principalmente en frutos, flores y hojas de las plantas, y son las responsables de conferir los colores rojo, azul y violeta. Se sintetizan a partir de la conversión de los precursores fenilalanina y acetato, vía el metabolismo del fenil propanoide, y se acumulan en las vacuolas de las células hipodermales. Las enzimas de la biosíntesis son reguladas a nivel de transcripción, y en uvas su punto crítico está a la altura del gen que codifica para la enzima UDP glucosa: flavonoide-3-glucosil transferasa (UFGT), lo que implica una regulación más tardía que en otras especies. Se sintetizan durante el cambio de color de la baya y son los pigmentos más importantes en las uvas coloreadas. Su estructura corresponde a heterociclos formados por la combinación de una aglicona y un azúcar. En Vitis vinifera esta se fija en la posición 3, y en otras Vitis son diglucósidos. Tienen una alta capacidad antioxidante, y han sido difíciles de estudiar debido a su inestabilidad, a pesar que la acetilación contribuye a su estabilización y son estas las que se almacenan más eficientemente en vacuolas. Su función se asocia con la coloración de la fruta y con la protección ante el estrés lumínico, como parte del sistema antioxidante. De ahí lo importante de su estudio. La concentración y perfil de antocianinas varían entre especies, cultivares, estados de madurez, condiciones estacionales, áreas de producción, prácticas culturales y niveles de rendimiento. La luminosidad y la temperatura son las principales variables ambientales que regulan síntesis de estos compuestos; la primera la estimula y las altas temperaturas parecen inhibirla. En el presente trabajo se hizo una revisión de los avances en la investigación sobre distintos aspectos de las antocianinas, tales como su naturaleza, ruta biosintética y regulación, además de los efectos que la luminosidad y la temperatura ejercen sobre ellas, en especial relación con uvas.
Relación entre el tipo de fisura labiopalatina y la disfunción de la trompa de Eustaquio en niños, Perú 2020
Authors: Antayhua Ortiz Sara Isabel 
Published: May 2022

Objetivo: Determinar si el tipo de fisura labiopalatina está asociada a mayor riesgo de alteración de la trompa de Eustaquio. Materiales y métodos: Se realizó un estudio observacional retrospectivo no experimental de los niños operados de fisura labiopalatina en el Hospital San Bartolomé durante el 2018 y 2019. Resultados: Se evaluó a un total de 48 pacientes a quienes se les realizó timpanometría previa a la cirugía de fisura palatina, encontrándose 62,5% varones (n=30) y 37,5% mujeres (n=18) y la media de edad fue de 1,3 años, para determinar el tipo de fisura labiopalatina se usó la clasificación de Veau, el tipo más frecuente hallado fue Veau tipo III, con 62,5% (n=30), siendo la menos frecuente Veau tipo I con 4,2% (n=2). La evaluación de la función de la trompa de Eustaquio se realizó mediante timpanometría definiéndose como patológica la curva tipo B, en el grupo de estudio se encontró una prevalencia global de curvas tipo B de 66,7% (n= 32), mientras que las curvas A y C, tuvieron 33,3% (n=16). Mediante la distribución binomial se determinó un valor fue de 0,4095 o 41%. La probabilidad que el diagnostico Veau IV tenga curvas tipo B de forma más frecuentes que las otros tipos, mediante la prueba Binomial fue significativo. (p=0,000038<0,05). Conclusiones: El tipo de fisura palatina mayormente asociado con alteración de la función del oído medio, expresado mediante curvas de timpanometría tipo B, es la fisura tipo IV según clasificación de Veau, esta relación es estadísticamente significativa.
Presencia de Escherichia coli en la playa Pucusana, Lima, y su potencial efecto en la salud pública
Authors: Huayanay Quevedo Cinthia Milagros , Aldoradin Basilio Veronica , Guerra Santa Cruz Alcides 
Published: May 2022

Objetivo: Determinar los valores de la bacteria e indicador fecal Escherichia coli para aguas de mar de uso seguro y recreacional de la playa Pucusana, Lima. Materiales y Métodos: Las campañas de muestreos se realizaron una vez por semana en el estío del 2019 y procesados en el Laboratorio de Microbiología de la FCB de la Universidad Ricardo Palma mediante colimetría y el método del número más probable NMP. Resultados: Se comprobó la presencia de esta especie en todas las muestras analizadas, con el valor crítico de 3000 NMP/100 ml. Diez cepas fueron enviadas al INS que determinó que no existe ningún serotipo patogénico, y de acuerdo con regulación peruana la calificación es inaceptable. Conclusiones: Se demuestra la carencia de salubridad ambiental y su relevancia en salud pública porque la disentería puede ser generada por este coliforme que afecta al crecimiento y desarrollo infantil e impacta en los adultos. Los factores de riesgo son producidos principalmente por la producción y vertido de las aguas residuales llevados por los colectores al mar y aunque importante proporción de estas aguas son procesadas en la Planta de Tratamiento de Aguas Residuales PTAR Pucusana que son reutilizadas en riego de áreas verdes y así poder integrar también su reúso en agricultura, se identificaron vertimientos domésticos y municipales que descargan al Pacífico, problemática que debe tener un buen manejo y fiscalización ambiental porque deteriora el medio ambiente sobre todo los mares y nuestras vidas dependen directamente de los océanos.
AKTIVITAS ANTIKANKER SERVIKS RUMPUT LAUT Sargassum sp
Authors: Tinanggal Perlina , Sanger Grace , Pandey Engel Victor , Makapedua Daisy Monica 
Published: May 2022

Kanker merupakan kondisi patologis yang menjadi salah satu utama penyebab kematian didunia. Selama beberapa dekade terakhir, langkah luar biasa telah dibuat untuk mengurangi insiden dan kematian kanker serviks dengan penerapan berbagai strategi pencegahan dan pengobatan. Tujuan penulisan artikel ilmiah untuk menemukan senyawa antikanker baru, yang sangat penting untuk pengobatan kanker dari rumput laut, karena rumput laut adalah salah satu produk laut sumber terbesar metabolit kimia aktif. Penelusuran literature menggunakan kata kunci, Sargassum sp, kanker serviks, HPV, obat antikanker serviks. Metode pengumpulan literature mengunakan elelectronic data base: PubMed, Web of Science, Science Direct, Elsevier, Springer Google and Google Scholar. Hasil yang diperoleh:, mekanisme senyawa antikanker melawan virus, aktifitas sitotoksik rumput laut melawan sel HeLa dan bebetapa jenis obat antikanker. Dapat disimpulkan bahwa Sargassum sp. mempunyai aktifitas sitotoksik melawan sel HeLa, sehingga dapat dijadikan sumber obat antikanker serviks. Rumput laut Sargassum sp. yang tumbuh melimpah di Indonesia dapat digunakan didalam pengobatan untuk menurunkan tingkat kasus dan kematian akibat kanker.
Kajian Mutu Ikan Layang (Decapterus russelli) yang Direndam dalam Asap Cair Sabut Kelapa dan Disimpan pada Suhu Ruang
Authors: Sarundayang Ratniarti , Salindeho Netty , Montolalu Lita , Pongoh Jenki , Montolalu Roike Iwan , Makapedua Daisy Monica 
Published: May 2022

Provinsi Sulawesi Utara, memiliki beberapa potensi perikanan pelagis yang dapat menunjang perekonomian masyarakat perikanan, salah satu komoditas perikanan pelagis yang berpotensi yaitu ikan layang (Decapterus russelli), Ikan merupakan bahan pangan yang mudah rusak karena mengandung protein dan air yang cukup tinggi. Oleh karena itu ikan harus segera diolah untuk mencegah terjadinya kemunduran mutu. Salah satu cara untuk mencegah terjadinya kerusakan pada ikan harus dilakukan pengawetan, teknik pengasapan ikan. Pengasapan merupakan salah satu cara pengolahan pangan yang telah lama dikenal sebagai salah satu tahapan dalam pengolahan produk pangan. Tujuan dari pengasapan ialah menghambat laju kerusakan produk, namun dalam perkembangan juga ditunjukan untuk memperoleh kenampakan tertentu pada produk asapan dan cita rasa asap pada bahan makanan. Penelitian ini bertujuan untuk mengetahui proses kemunduran mutu produk ikan layang asap cair selama perendaman dan lama penyimpanan suhu ruang berdasarkan nilai Ph, kadar air dan cemaran mikroba dengan menghitung nilai angka lempeng total (ALT), data yang diperoleh dari hasil penelitian yang dilakukan dihitung nilai rata-ratanya, lalu dilanjutkan dengan uji ANOVA pada aplikasi SPSS. Hasil analisa ikan layang asap cair diperoleh nilai angka lempeng total 1.8 x 107 – 1.6 x 1010, kadar air 39.5% – 62.7%, dan stabilitas pH 5.78 – 7.1
A IMPORTÂNCIA DE ESTUDOS DE AVALIAÇÃO E PERCEPÇÕES SOCIOLINGUÍSTICAS
Authors: Oushiro Lívia 
Published: May 2022

Este trabalho busca argumentar que (i) assim como a produção linguística, as avaliações e as percepções são demonstravelmente variáveis e ordenadas; e (ii) os estudos sobre avaliações e percepções são fundamentais não só para a compreensão sobre processos de variação e mudança linguística (Weinreich, Labov & Herzog, 2006 [1968]), mas também para a promoção do respeito linguístico (Scherre, 2020). Para tanto, resenham-se inicialmente alguns estudos que tratam de avaliações e de percepções, e que demonstram a sistematicidade de seus padrões de variação; em seguida, apresenta-se sinteticamente um método para modelagem de campos indexicais (Eckert, 2008), conceito que permite operacionalizar os múltiplos significados sociais de variantes linguísticas. Argumenta-se, em última instância, que os próprios linguistas ainda pouco conhecem sobre os mecanismos de associação entre certas variantes e significados sociais, e que a ampliação de estudos sistemáticos sobre avaliações e percepções sociolinguísticas é peça-chave para o combate ao preconceito linguístico e a promoção da diversidade linguística.
Palavras-chave: avaliações e percepções linguísticas; significados sociais; respeito linguístico.
VARIAÇÃO DISCURSIVA E ENSINO DE LÍNGUA PORTUGUESA: CONECTORES EM TEXTOS NARRATIVOS ESCRITOS
Authors: Tavares Maria Alice 
Published: May 2022

Neste texto, mostro que pesquisas sobre variáveis discursivas são excelentes fontes de informações para o tratamento de itens discursivos nas escolas de nível básico. Os exemplos provêm de pesquisas sobre os conectores E e AÍ. Estudos sociolinguísticos (ABREU, 1992; GÖRSKI; TAVARES, 2013; FREITAG et al., 2013, entre outros) observaram problemas no uso desses conectores em textos escritos por alunos de diferentes níveis de escolaridade. Por um lado, há uma tendência de alta frequência do AÍ em textos de indivíduos em fases iniciais de escolarização. Por outro lado, há uma tendência de alta frequência do E em textos de indivíduos com maior tempo de escolarização. Com base nessas descobertas, apresento sugestões para a abordagem a conectores no ensino básico, distribuídas em três eixos: (i) experienciação e análise linguística; (ii) avaliação sociolinguística; e (ii) aplicação na produção de textos. Defendo que a adoção de um embasamento sociolinguístico para o ensino de língua portuguesa nos permite explorar com profundidade características morfossintáticas, semânticopragmáticas e estilísticas de itens discursivos.
Palavras-chave: Variação discursiva. Ensino. Conectores.
PERCEPÇÕES DOS GESTORES ESCOLARES E PROFESSORES SOBRE AS IMPLICAÇÕES DAS VISITAS DE SUPERVISÃO PEDAGÓGICA
Authors: Gomundanhe Almeida Meque , Choe Joao Francisco de Carvalho 
Published: May 2022

Este artigo tem como tema percepções dos gestores escolares e professores sobre as implicações das visitas de supervisão pedagógica: estudo de caso da Escola Primária completa Eduardo mondlane-cidade de chimoio. Por isso, o objectivo deste estudo consiste em analisar as percepções dos gestores escolares e professores sobre as implicações das visitas de supervisão pedagógica na Escola Primária Completa Eduardo Mondlane, localizada na cidade de Chimoio, em Moçambique. Metodologicamente, adoptou-se uma abordagem qualitativa e quantitativa associada a revisão bibliográfica e as técnicas de questionário e a observação direta. O questionário foi aplicado a 10 professores, um director da escola, um director adjunto pedagógico, um coordenador do 1º ciclo e a uma coordenadora do 2º ciclo. Os resultados obtidos mostraram que a supervisão pedagógica contribui para o desenvolvimento profissional dos professores por meio das interações, trocas de experiência entre o supervisor e o professor.