bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ubiquitin C‐terminal hydrolase L1 (UCHL1), a double‐edged sword in mammalian oocyte maturation and spermatogenesis
Authors: Yang Donghui , Lu Qizhong , Peng Sha , Hua Jinlian 
Published: October 2022

AbstractBackgroundRecent studies have shown that ubiquitin‐mediated cell apoptosis can modulate protein interaction and involve in the progress of oocyte maturation and spermatogenesis. As one of the key regulators involved in ubiquitin signal, ubiquitin C‐terminal hydrolase L1 (UCHL1) is considered a molecular marker associated with spermatogonia stem cells. However, the function of UCHL1 was wildly reported to regulate various bioecological processes, such as Parkinson's disease, lung cancer, breast cancer and colon cancer, how UCHL1 affects the mammalian reproductive system remains an open question.MethodsWe identified papers through electronic searches of PubMed database from inception to July 2022.ResultsHere, we summarize the important function of UCHL1 in controlling mammalian oocyte development, regulating spermatogenesis and inhibiting polyspermy, and we posit the balance of UCHL1 was essential to maintaining reproductive cellular and tissue homeostasis.ConclusionThis study considers the ‘double‐edged sword’ role of UCHL1 during gametogenesis and presents new insights into UCHL1 in germ cells.
Early onset of azithromycin to prevent CLAD in lung transplantation: Promising results of a retrospective single centre experience
Authors: Cristeto Porras Marta , Mora Cuesta Víctor Manuel , Iturbe Fernández David , Tello Mena Sandra , Alonso Lecue Pilar , Sánchez Moreno Laura , Miñambres García Eduardo , Naranjo Gozalo Sara , Izquierdo Cuervo Sheila , Cifrián Martínez José Manuel 
Published: October 2022

AbstractIntroductionAzithromycin (AZI) may be an effective immune modulator in lung transplant (LT) recipients, and can decrease chronic lung allograft dysfunction (CLAD) rates, the leading cause of mortality after the 1st year post‐LT. The aim of the study is to assess the effect of AZI initiation and its timing on the incidence and severity of CLAD in LT recipients.MethodsSingle‐center retrospective study, including LT recipients from 01/01/2011 to 30/06/2020. Four groups were established: those who started AZI at the 3rd week post‐LT (group A), those who received AZI later than the 3rd week post‐LT and had preserved FEV1 (B), those who did not receive AZI (C) and those who started AZI due to a decline in FEV1 (D). The dosage of AZI prescribed was 250 mg three times per week. CLAD was defined and graduated according to the 2019 ISHLT criteria.ResultsWe included 358 LT recipients: 139 (38.83%) were in group A, 94 (26.25%) in group B, 91 (25.42%) in group C, and 34 (9.50%) in group D. Group A experienced the lowest CLAD incidence and severity at 1 (p = .01), 3 (p < .001), and 5 years post‐LT, followed by Group B. Groups C and D experienced a higher incidence and severity of CLAD (p = .015). Initiation of AZI prior to FEV1 decline (Groups A and B) proved to be protective against CLAD after adjusting for differences between the treatment groups.ConclusionsEarly initiation of AZI in LT recipients could have a role in decreasing the incidence and severity of CLAD. In addition, as long as FEV1 is preserved, initiating AZI at any time could also be useful to prevent the incidence of CLAD and reduce its severity.
Potential of temperature‐response collagen‐genipin sol as a novel submucosal injection agent for endoscopic resection: Acute and chronic phase study using living animals
Authors: Takatori Yusaku , Uraoka Toshio , Sasaki Motoki , Narita Takefumi , Yunoki Shunji , Shiraishi Junichi , Yahagi Naohisa 
Published: October 2022

ObjectivesWe proposed a novel temperature‐response collagen sol as a submucosal injection agent for endoscopic resection (ER) using pepsin‐solubilized collagen (PSC) and genipin (Ge) in a prior study. This study aimed to evaluate the usefulness and safety of the sol (PSC/Ge) in acute and chronic phase experiments using living animals.MethodsIn experiment 1, we performed endoscopic submucosal dissection (ESD) for six pigs using normal saline (NS), sodium hyaluronate (SH), and PSC/Ge. We compared the required amount of each agent per unit area and procedure time. In experiment 2, we created artificial ulcers with endoscopic mucosal resection (EMR) for five pigs using NS and PSC sol. We compared the artificial ulcer residual rate at 7 and 14 days after EMR, and the scarring rate at 14 days after EMR.ResultsThe required amount of agents per unit area for PSC/Ge (0.8 ± 0.8 mL/cm2) and SH (1.1 ± 0.8 mL/cm2) were significantly smaller than that for NS (1.8 ± 0.7 mL/cm2). The total procedure time did not have a statistical difference. The artificial ulcer residual rates were 47.3 ± 0.7% for NS and 40.3 ± 0.7% for PSC/Ge on day 7 (P = 0.51), and 15.0 ± 0.1% for NS and 10.2 ± 0.1% for PSC/Ge sol on day 14 (P = 0.35). The scarring rate on day 14 was 10% for NS and 20% for PSC/Ge.ConclusionWe demonstrated the feasibility of a novel temperature‐response collagen gel as a submucosal injection agent for ER in the acute and chronic phase animal experiment.
An omics evolutionary perspective on phytophagous insect–host plant interactions in Anastrepha obliqua: a review
Authors: Velasco‐Cuervo Sandra M. , Galindo‐González Leonardo , Toro‐Perea Nelson 
Published: October 2022

AbstractPhytophagous insects have a close relationship with their host plants. For this reason, their interactions can lead to important changes in insect population dynamics and evolutionary trajectories. Next generation sequencing (NGS) has provided an opportunity to analyze omics data on a large scale, facilitating the change from a classical genetics approach to a more holistic understanding of the underlying molecular mechanisms of host plant use by insects. Most studies have been carried out on model species in Holarctic and temperate zones. In tropical zones, however, the effects of use of various host plants on evolutionary insect history is less understood. In the current review, we describe how omics methodologies help us to understand phytophagous insect–host plant interactions from an evolutionary perspective, using as example the Neotropical phytophagous insect West Indian fruit fly, Anastrepha obliqua (Macquart) (Diptera: Tephritidae), an economically important fruit crop pest in the Americas. Anastrepha obliqua could adopt a generalist or a specialist lifestyle. We first review the adaptive molecular mechanisms of phytophagous insects to host plants, and then describe the main tools to study phytophagous insect–host plant interactions in the era of omics sciences. The omics approaches will advance the understanding of insect molecular mechanisms and their influence on diversification and evolution. Finally, we discuss the importance of a multidisciplinary approach that integrates the use of omics tools and other, more classical methodologies in evolutionary studies.
Differential susceptibility of two locust pests to the microsporidium Paranosema (Antonospora) locustae at suboptimal rearing temperature
Authors: Tokarev Yuri S. , Gerus Aleksei V. , Pavlyushin Vladimir A. 
Published: October 2022

AbstractParanosema (Antonospora) locustae (Canning) is a microsporidium with an extensive host range including >100 reported insect hosts from the order Orthoptera. The susceptibility of two species of locusts (Orthoptera: Acrididae) – the migratory locust, Locusta migratoria subsp. migratorioides (Fairmaire & Reiche), and the desert locust, Schistocerca gregaria Forsskål – to P. locustae was compared under laboratory conditions at a decreased temperature of 27 °C to enhance susceptibility to infection. Locusta migratoria was found highly vulnerable as infection percentages exceeded 70% at 104, 105, and 106 spores per insect, and mortality increased with increasing dosage. Conversely, P. locustae spores were not found in S. gregaria throughout the experiment. Only at 107 spores per insect, as many as 20% of S. gregaria were infected. This suggests that the desert locust is resistant to P. locustae infection, as opposed to the migratory locust. The laboratory models of these parasite–host systems may be useful for elucidating mechanisms of insect immunity to microsporidia.
An experimental study on post‐mortem dissolution and overgrowth processes affecting coccolith assemblages: A rapid and complex process
Authors: Holcová Katarína , Scheiner Filip 
Published: October 2022

AbstractCoccolith dissolution together with post‐mortem morphological features are immensely important phenomena that can affect assemblage compositions, complicate taxonomic identification as well as provide valuable palaeoenvironmental insights. This study summarizes the effects of pH oscillations on post‐mortem coccolith morphologies and the abundances and compositions of calcareous nannoplankton assemblages in three distinct types of material—(i) Cretaceous chalk, (ii) Miocene marls, and (iii) late Holocene calcareous ooze. Two independent experimental runs within a semi‐enclosed system setting were realized to observe assemblage alterations. One experiment was realized with the presence of bacteria and, in contrast, the second one inhibited their potential effect on the studied system. The pH was gradually decreased within the range of 8.3–6.4 using a reaction of CO2 with H2O forming weak carbonic acid (H2CO3), thereby affecting []. Further, a subsequent overgrowth study was carried out during spontaneous degassing accompanied by a gradual pH rise. The experiment revealed that the process and intensity of coccolith corrosion and subsequent overgrowth build‐ups are influenced by a plethora of different factors such as (i) pH and associated seawater chemistry, (ii) mineral composition of the sediment, (iii) the presence of coccoliths within a protective substrate (faecal pellets, pores, pits), and (iv) the presence/absence of bacteria. Nannoplankton assemblages with corroded coccoliths or with coccoliths with overgrowth build‐ups showed that the observed relative abundances of taxa experienced alteration from the original compositions. Additionally, extreme pH oscillations may result in enhanced morphological changes that make coccoliths unidentifiable structures, and might even evoke the absence of coccoliths in the fossil record.
Prognostic impact of tumour budding in squamous cell carcinoma of the lung: a systematic review and meta‐analysis
Authors: Wankhede D , Hofman P , Grover S 
Published: October 2022

Tumour budding is an established prognostic factor in various solid tumours, including colorectal cancers and oral squamous cell carcinomas. However, its role is unclear and needs to be defined for squamous cell carcinoma of the lung (LSCC). Hence, we conducted a systematic review and meta‐analysis investigating the prognostic role of tumour budding in LSCC. PubMed, Embase and Scopus were searched for peer‐reviewed literature investigating the association between tumour budding and survival outcomes or clinicopathological variables in LSCC. The primary outcomes were pooled estimates for overall and recurrence‐free survival with hazard ratio (HR) as the effect measure. The association between tumour budding and clinicopathological parameters was also investigated. Of 243 studies, nine were included, comprising 2546 patients. An increased risk of death [HR = 1.76, 95% confidence interval (CI) = 1.50–2.05, P < 0.00001] and recurrence (HR = 1.37, 95% CI = 1.12–1.68, P = 0.003) was evident in patients with high‐grade tumour budding. Sensitivity and subgroup analyses revealed consistent results. Pathological stage II, lymph node metastasis, lymphovascular and pleural invasion were associated with high‐grade tumour budding. Tumour budding is a new and promising prognostic factor in patients with LSCC. However, pervasive heterogeneity and publication bias reduces the credibility of these findings and the applicability of tumour budding in clinical practice. Future studies are required to standardise reporting on tumour budding in LSCC.
A high‐dimensional cytometry atlas of peripheral blood over the human life span
Authors: Jalali Sedigheh , Harpur Christopher M , Piers Adam T , Auladell Maria , Perriman Louis , Li Shuo , An Kim , Anderson Jeremy , Berzins Stuart P , Licciardi Paul V , Ashhurst Thomas M , Konstantinov Igor E , Pellicci Daniel G 
Published: October 2022

AbstractAge can profoundly affect susceptibility to a broad range of human diseases. Children are more susceptible to some infectious diseases such as diphtheria and pertussis, while in others, such as coronavirus disease 2019 and hepatitis A, they are more protected compared with adults. One explanation is that the composition of the immune system is a major contributing factor to disease susceptibility and severity. While most studies of the human immune system have focused on adults, how the immune system changes after birth remains poorly understood. Here, using high‐dimensional spectral flow cytometry and computational methods for data integration, we analyzed more than 50 populations of immune cells in the peripheral blood, generating an immune cell atlas that defines the healthy human immune system from birth up to 75 years of age. We focused our efforts on children under 18 years old, revealing major changes in immune cell populations after birth and in children of schooling age. Specifically, CD4+ T effector memory cells, Vδ2+ gamma delta (γδ)T cells, memory B cells, plasmablasts, CD11c+ B cells and CD16+CD56bright natural killer (NK) cells peaked in children aged 5–9 years old, whereas frequencies of T helper 1, T helper 17, dendritic cells and CD16+CD57+CD56dim NK cells were highest in older children (10–18 years old). The frequency of mucosal‐associated invariant T cells was low in the first several years of life and highest in adults between 19 and 30 years old. Late adulthood was associated with fewer mucosal‐associated invariant T cells and Vδ2+ γδ T cells but with increased frequencies of memory subsets of B cells, CD4+ and CD8+ T cells and CD57+ NK cells. This human immune cell atlas provides a critical resource to understand changes to the immune system during life and provides a reference for investigating the immune system in the context of human disease. This work may also help guide future therapies that target specific populations of immune cells to protect at‐risk populations.
Influence of low insertion torque values on survival rate of immediately loaded dental implants: A systematic review and meta‐analysis
Authors: Darriba Iria , Seidel Anna , Moreno Federico , Botelho João , Machado Vanessa , Mendes José João , Leira Yago , Blanco Juan 
Published: October 2022

AbstractAimThe aim was to systematically evaluate the effect of low insertion torque values on the survival rate of immediately loaded dental implants.Materials and MethodsThe protocol was registered with PROSPERO (ID CRD42020189499). An electronic search was performed in PubMed, Embase, Web of Science, and Cochrane Central Register of Controlled Trials until June 2022 in English and Spanish. Studies analysing the failure or survival rate of immediately loaded dental implants according to different insertion torque values were included.ResultsFive‐hundred seventy‐three articles were assessed for eligibility, of which seven articles, four randomized clinical trials (RCTs), one controlled clinical trial, and two prospective case series studies were included in the qualitative analysis. The RCTs were classified as having low risk of bias and the non‐RCTs as having moderate and serious risk of bias. The mean survival rate for implants with low insertion toque (≤35 Ncm) was 96% (p > .001, 95% confidence interval [CI]: 0.91–0.98) and that for implants with medium or high insertion torque (>35 Ncm) was 92% (p > .001, 95% CI: 0.86–0.96) (incidence rate ratio [IRR] = 1.05, 95% CI: 0.79–1.39, p = .175, I2 = 0.0%). Splinted implants with insertion torque >20 Ncm and single implants with insertion torque >35 Ncm had a higher survival rate than implants with lower insertion torque values (IRR = 1.05, 95% CI: 0.78–1.43, p = .956, I2 = 0.0%, and RR = 0.92, 95% CI: 0.48–1.75, p = .799, I2 = 0.0%, respectively). Different insertion torque values achieved equivalent outcomes. The mean follow‐up was 24 months.ConclusionsLow insertion torque values have no significant effect on survival rates of immediate loading implants at a mean follow‐up of 24 months.
Improving patient and carer access to information and support through head and neck cancer treatment and survivorship using experience‐based co‐design
Authors: Hiatt Joanne , Young Adrienne , Brown Teresa , Banks Merrilyn , Bauer Judith 
Published: October 2022

AbstractBackgroundPrevious studies have highlighted the unmet nutritional and supportive care needs of patients with head and neck cancer (HNC) and their carers from diagnosis and throughout the treatment and survivorship period. The aim of this study was to bring patients, carers and healthcare professionals together to co‐design a framework to improve access to nutrition information and support for patients and carers with HNC from diagnosis and throughout the treatment and survivorship period.MethodsUsing experience‐based co‐design (EBCD), semistructured individual interviews were conducted with patients, carers and healthcare professionals to understand their experiences in accessing information and support outside of the hospital environment. Feedback events and co‐design workshops were held to prioritise areas for service improvement.ResultsParticipants (10 patients, 7 carers and 15 healthcare professionals) highlighted the importance of having consistent information and support recommendations from the multidisciplinary team. The two key areas for improvement identified through group and workshop events were linking reputable HNC resources to a HNC portal on the hospital website and the development of a series of short podcasts and video blogs with fact sheets attached presented by members of the multidisciplinary team, patients and carers at four time points spanning pretreatment and throughout the survivorship period.ConclusionsUsing EBCD has enabled the co‐design of a framework for resource development with patients, carers and healthcare professionals to improve access to information and resources to support nutrition intake and supportive care needs for patients with HNC with their carers. Development and implementation of resources and evaluation of outcomes is ongoing.
LRRK2 expression in normal and pathologic human gut and in rodent enteric neural cell lines
Authors: de Guilhem de Lataillade Adrien , Caillaud Martial , Oullier Thibauld , Naveilhan Philippe , Pellegrini Carolina , Tolosa Eduardo , Neunlist Michel , Rolli‐Derkinderen Malvyne , Gelpi Ellen , Derkinderen Pascal 
Published: October 2022

AbstractLeucine‐rich repeat kinase 2 (LRRK2) gene, which is the gene most commonly associated with Parkinson's disease (PD), is also a susceptibility gene for Crohn's disease, thereby suggesting that LRRK2 may sit at the crossroads of gastrointestinal inflammation, Parkinson's, and Crohn's disease. LRRK2 protein has been studied intensely in both CNS neurons and in immune cells, but there are only few studies on LRRK2 in the enteric nervous system (ENS). LRRK2 is present in ENS ganglia and the existing studies on LRRK2 expression in colonic biopsies from PD subjects have yielded conflicting results. Herein, we propose to extend these findings by studying in more details LRRK2 expression in the ENS. LRRK2 expression was evaluated in full thickness segments of colon of 16 Lewy body, 12 non‐Lewy body disorders cases, and 3 non‐neurodegenerative controls and in various enteric neural cell lines. We showed that, in addition to enteric neurons, LRRK2 is constitutively expressed in enteric glial cells in both fetal and adult tissues. LRRK2 immunofluorescence intensity in the myenteric ganglia was not different between Lewy body and non‐Lewy body disorders. Additionally, we identified the cAMP pathway as a key signaling pathway involved in the regulation of LRRK2 expression and phosphorylation in the enteric glial cells. Our study is the first detailed characterization of LRRK2 in the ENS and the first to show that enteric glial cells express LRRK2. Our findings provide a basis to unravel the functions of LRRK2 in the ENS and to further investigate the pathological changes in enteric synucleinopathies.image
Nurse's, physician's and family member's experiences of withholding or withdrawing life‐sustaining treatment process in an intensive care unit
Authors: Choi Hye Ri , Rodgers Sheila , Tocher Jennifer , Kang Sung Wook 
Published: October 2022

AbstractAimsTo explore nurse's, physician's and family member's experiences of withholding or withdrawing life‐sustaining treatment in an intensive care unit.BackgroundIn South Korea, withholding or withdrawing life‐sustaining treatment is legalised by the enforcement of the Hospice, Palliative Care and Life‐sustaining Treatment Decision‐making Act (2018). The Act (2018) is the first legal ground for making decisions regarding life‐sustaining treatment in South Korea.DesignFocused ethnography. The standards for reporting qualitative research checklist is used.MethodsInterview data are collected between August 2018 and January 2019 using semi‐structured interviews with 23 nurses, 10 physicians and four family members in a South Korean intensive care unit. The interview data are analysed following the thematic analysis of Braun and Clarke.ResultsAn overarching theme of ‘constructing death’ is identified from the experiences of nurses, physicians and family members regarding withholding or withdrawing life‐sustaining treatment in a South Korean intensive care unit. Family members had the strongest power in the withholding or withdrawing life‐sustaining treatment process whilst the process had to be based on medical consideration. All the research participants shared the purpose and motivation of withholding or withdrawing life‐sustaining treatment as the dying patient's dignity. Due to the South Korean national health insurance system, the relationships between medical staff and family members were driven by customer ideology.ConclusionThe impact and linkage of the context of familism culture and health insurance with the process of withholding or withdrawing life‐sustaining treatment in South Korea are shown in this research. The findings of this research inspire future studies to uncover the impact of the cultural context in the decision‐making process of a patient's death, to explore the dynamics of family members under cultural values and to explore the influence of the healthcare system and medical costs on the relationships between medical staff and family members.Relevance to Clinical PracticeBy integrating the experiences of nurses, physicians and family members, the findings of this study inform the shared values in the context of familism culture and the health insurance system. In particular, understanding family dynamics when a patient's dying and death as a result of withholding or withdrawing life‐sustaining treatment informs nurses to provide quality of care in the intensive care setting. Therefore, the findings of this research contribute to distinguishing the priority in care when withholding or withdrawing life‐sustaining treatment, rapidly changing the aims of care from the patient's recovery to a dignified death.
Import Competition and Household Debt
Authors: BARROT JEAN‐NOËL , LOUALICHE ERIK , PLOSSER MATTHEW , SAUVAGNAT JULIEN 
Published: October 2022

ABSTRACTWe analyze the effect of import competition on household balance sheets using individual data on consumer finances. We exploit variation in local industry exposure to foreign competition to study households' response to the income shock triggered by China's accession to the World Trade Organization. We show that household debt increases significantly in regions where manufacturing industries are more exposed to import competition. The effects are driven by home equity extraction and are concentrated in areas with strong house price growth. Our results highlight the role played by mortgage markets in absorbing displacement shocks triggered by globalization.