bluuebunny/crossref_metadata_2025_split huggingface dataset:
The radiographic outcome after plating for pubic symphysis diastasis: does it matter clinically?
Authors: Tseng Kuo-Yuan , Lin Kai-Cheng , Yang Shan-Wei 
Published: March 2022

Abstract
Introduction
Open reduction and internal fixation with plates is the most widespread surgery in traumatic pubic symphysis diastasis. However, implant failure or recurrent diastasis was commonly observed during follow-up. The aim of our study was to evaluate the radiologic findings and clinical outcomes.

Materials and methods
Sixty-five patients with traumatic pubic symphysis diastasis treated with plating between 2008 and 2019 were retrospectively reviewed. The exclusion criteria were a history of malignancy and age under 20 years. Radiographic outcomes were determined by radiograph findings, including pubic symphysis distance (PSD) and implant failure. Clinical outcomes were assessed according to the Majeed score at the final follow-up.

Results
Twenty-eight patients were finally included. Nine patients (32%) experienced implant failure, including four (14%) with screw loosening and five (18%) with plate breakage. Only one patient underwent revision surgery. Postoperatively, a significant increase in PSD was observed at 3 months and 6 months. Postoperative PSD was not significantly different between patients with single plating and double plating, but it was significantly greater in the implant-failure group than in the non-failure group. The Majeed score was similar between patients with single plating and double plating or between the implant-failure group and the non-failure group. Body mass index, number of plates, age, and initial injured PSD were not significantly different between the implant-failure group and the non-failure group. Only a significant male predominance was observed in the implant-failure group.

Conclusion
A gradual increase in the symphysis distance and a high possibility of implant failure may be the distinguishing features of traumatic pubic symphysis diastasis fixation.
The postoperative symphyseal distance achieved stability after 6 months, even after implant failure. Radiographic outcomes, such as increased symphysis distance, screw loosening, and plate breakage, did not affect clinical functional outcomes.
The rights of migrants to the identification of their dead: an attempt at an identification strategy from Italy
Authors: Cattaneo Cristina , De Angelis Danilo , Mazzarelli Debora , Porta Davide , Poppa Pasquale , Caccia Giulia , D’Amico Maria Elisa , Siccardi Cecilia , Previderè Carlo , Bertoglio Barbara , Tidball-Binz Morris , Ubelaker Douglas , Piscitelli Vittorio , Riccio Silvana 
Published: March 2022

AbstractEurope is turning a blind eye on a humanitarian disaster unfolding at its doorsteps, with thousands of migrants dying unidentified in Mediterranean waters. Since 2014, Italy has been struggling in an almost indifferent international scenario to identify its dead migrants. Despite the lack of sufficient resources, of the difficulties in collecting post mortem data from the disseminated bodies, and of the problems of contacting and collecting ante mortem information from relatives, it has been proven, with a series of pilot studies, that not only can these bodies be identified but that relatives are also looking for their loved ones and need death certificates. This article focuses on the administrative limbo and lack of regulations obliging single states to engage in appropriate procedures to maximise identification.
In situ simulation training for parental presence during critical situations in PICU: an observational study
Authors: Bordessoule Alice , Felice-Civitillo Cristina , Grazioli Serge , Barcos Francisca , Haddad Kevin , Rimensberger Peter C. , Polito Angelo 
Published: March 2022

AbstractFamily presence during invasive procedures or cardiopulmonary resuscitation (CPR) is a part of the family-centered approach in pediatric intensive care units (PICUs). We established a simulation program aiming at providing communication tools to healthcare professionals. The goal of this study was to evaluate the impact of this program on the stress of PICU professionals and its acceptance. An observational study of a simulation program, with questionnaire, was used to measure pre- and post-simulation stress and the degree of satisfaction of the participants. PICU of Geneva Children’s Hospital, Switzerland. Forty simulations with four different simulation scenarios and various types of parental behavior, as imitated by professional actors, were completed during a 1-year period. Primary outcomes were the difference in perceived stress level before and after the simulation and the degree of satisfaction of healthcare professionals (nursing assistants, nurses, physicians). The impact of previous experience with family members during critical situations or CPR was evaluated by variation in perceived stress level. Overall, 201 questionnaires were analyzed. Perceived stress associated with parental presence decreased from a pre-simulation value of 6 (IQR, 4–7) to 4 (IQR, 2–5) post-simulation on a scale of 1–10. However, in 25.7% of cases, the individually perceived post-simulation stress level was higher than the pre-simulation one. Satisfaction of the participants was high with a median of 10 (IQR, 9–10) out of 10.Conclusions: A simulation program helps reduce PICU team emotional stress associated with the presence of family members during critical situations or CPR, and is welcomed by PICU team members.

What is Known:• Family presence during cardiopulmonary resuscitation (CPR) or critical situations is a part of the family-centered approach in pediatric intensive care.• The benefits for the family have been already demonstrated. However, this policy is still controversy among healthcare professionals.

What is New:• A simulation program seeking to provide skills focused on family presence management in the PICU is useful to reduce stress and was well accepted by participants.• It might become an indispensable training intervention for the implementation of a PICU policy to allow family presence during CPR or other critical situations.
Autosomal dominant non-syndromic hearing loss maps to DFNA33 (13q34) and co-segregates with splice and frameshift variants in ATP11A, a phospholipid flippase gene
Authors: Pater Justin A. , Penney Cindy , O’Rielly Darren D. , Griffin Anne , Kamal Lara , Brownstein Zippora , Vona Barbara , Vinkler Chana , Shohat Mordechai , Barel Ortal , French Curtis R. , Singh Sushma , Werdyani Salem , Burt Taylor , Abdelfatah Nelly , Houston Jim , Doucette Lance P. , Squires Jessica , Glaser Fabian , Roslin Nicole M. , Vincent Daniel , Marquis Pascale , Woodland Geoffrey , Benoukraf Touati , Hawkey-Noble Alexia , Avraham Karen B. , Stanton Susan G. , Young Terry-Lynn 
Published: March 2022

AbstractSequencing exomes/genomes have been successful for identifying recessive genes; however, discovery of dominant genes including deafness genes (DFNA) remains challenging. We report a new DFNA gene, ATP11A, in a Newfoundland family with a variable form of bilateral sensorineural hearing loss (SNHL). Genome-wide SNP genotyping linked SNHL to DFNA33 (LOD = 4.77), a locus on 13q34 previously mapped in a German family with variable SNHL. Whole-genome sequencing identified 51 unremarkable positional variants on 13q34. Continuous clinical ascertainment identified several key recombination events and reduced the disease interval to 769 kb, excluding all but one variant. ATP11A (NC_000013.11: chr13:113534963G>A) is a novel variant predicted to be a cryptic donor splice site. RNA studies verified in silico predictions, revealing the retention of 153 bp of intron in the 3′ UTR of several ATP11A isoforms. Two unresolved families from Israel were subsequently identified with a similar, variable form of SNHL and a novel duplication (NM_032189.3:c.3322_3327+2dupGTCCAGGT) in exon 28 of ATP11A extended exon 28 by 8 bp, leading to a frameshift and premature stop codon (p.Asn1110Valfs43Ter). ATP11A is a type of P4-ATPase that transports (flip) phospholipids from the outer to inner leaflet of cell membranes to maintain asymmetry. Haploinsufficiency of ATP11A, the phospholipid flippase that specially transports phosphatidylserine (PS) and phosphatidylethanolamine (PE), could leave cells with PS/PE at the extracellular side vulnerable to phagocytic degradation. Given that surface PS can be pharmaceutically targeted, hearing loss due to ATP11A could potentially be treated. It is also likely that ATP11A is the gene underlying DFNA33.
Basement membrane defects in CD151-associated glomerular disease
Authors: Naylor Richard W. , Watson Elizabeth , Williamson Samantha , Preston Rebecca , Davenport J Bernard , Thornton Nicole , Lowe Martin , Williams Maggie , Lennon Rachel 
Published: March 2022

Abstract

Background
CD151 is a cell-surface molecule of the tetraspanin family. Its lateral interaction with laminin-binding integrin ɑ3β1 is important for podocyte adhesion to the glomerular basement membrane (GBM). Deletion of Cd151 in mice induces glomerular dysfunction, with proteinuria and associated focal glomerulosclerosis, disorganisation of GBM and tubular cystic dilation. Despite this, CD151 is not routinely screened for in patients with nephrotic-range proteinuria. We aimed to better understand the relevance of CD151 in human kidney disease.

Methods
Next-generation sequencing (NGS) was used to detect the variant in CD151. Electron and light microscopy were used to visualise the filtration barrier in the patient kidney biopsy, and immunoreactivity of patient red blood cells to anti-CD151/MER2 antibodies was performed. Further validation of the CD151 variant as disease-causing was performed in zebrafish using CRISPR-Cas9.

Results
We report a young child with nail dystrophy and persistent urinary tract infections who was incidentally found to have nephrotic-range proteinuria. Through targeted NGS, a novel, homozygous truncating variant was identified in CD151, a gene rarely reported in patients with nephrotic syndrome. Electron microscopy imaging of patient kidney tissue showed thickening of GBM and podocyte effacement. Immunofluorescence of patient kidney tissue demonstrated that CD151 was significantly reduced, and we did not detect immunoreactivity to CD151/MER2 on patient red blood cells. CRISPR-Cas9 depletion of cd151 in zebrafish caused proteinuria, which was rescued by injection of wild-type CD151 mRNA, but not CD151 mRNA containing the variant sequence.

Conclusions
Our results indicate that a novel variant in CD151 is associated with nephrotic-range proteinuria and microscopic haematuria and provides further evidence for a role of CD151 in glomerular disease. Our work highlights a functional testing pipeline for future analysis of patient genetic variants.

Graphical abstract
A higher resolution version of the Graphical abstract is available as Supplementary information
Disparities in quality of life, social distress and employment outcomes in Australian cancer survivors
Authors: White Victoria M. , Lisy Karolina , Ward Andrew , Ristevski Eli , Clode Melanie , Webber Kate , Emery Jon , Ijzerman Maarten J. , Afshar Nina , Millar Jeremy , Gibbs Peter , Evans Sue , Jefford Michael 
Published: March 2022

Abstract

Purpose

To examine how socio-demographic, comorbidities and information needs influence quality of life (QoL) outcomes of survivors of breast, colorectal, or prostate cancer, non-Hodgkin lymphoma or melanoma.

Methods
Cross-sectional postal survey with eligible participants identified through a population-based cancer registry. QoL outcomes were assessed by EQ-5D-5L, social difficulties index (SDI) and, for those employed at diagnosis, current employment. Regression analyses explored associations between outcome variables and cancer type, age, time since diagnosis, residential location, socio-economic disadvantage, comorbidities and unmet information needs. Mediation analyses examined whether comorbidities and information needs explained relationships between outcome variables and socio-economic disadvantage.

Results
2115 survivors participated. Mean EQ-5D-5L scores (mean = 0.84) were similar to population averages and SDI scores were low for the entire sample (mean = 3.80). In multivariate analyses, being aged over 80, greater socio-economic disadvantage, comorbidities and unmet information needs decreased EQ-5D-5L scores. Higher SDI scores were associated with socio-economic disadvantage, comorbidities and unmet information needs. Not being employed was associated with being aged over 50, more comorbidities and socio-economic disadvantage. Comorbidities but not information needs partially mediated the impact of socio-economic disadvantage on EQ-5D-5L and SDI accounting for 17% and 14% of the total effect of socio-economic disadvantage respectively. Neither comorbidities nor information needs mediated the association between socio-economic disadvantage and employment outcomes.

Conclusions
To improve quality of life, survivorship care should be better tailored to address the needs of individuals given their overall health and impact of comorbidities, their age and type of cancer and not simply time since diagnosis.
Tooth extractions prior to chemoradiation or bioradiation are associated with weight loss during treatment for locally advanced oropharyngeal cancer
Authors: Buurman Doke J. M. , Willemsen Anna C. H. , Speksnijder Caroline M. , Baijens Laura W. J. , Hoeben Ann , Hoebers Frank J. P. , Kessler Peter , Schols Annemie M. W. J. 
Published: March 2022

Abstract

Purpose
Prior to radiotherapy combined with chemotherapy (CRT) or biotherapy (BRT) for oropharyngeal squamous cell carcinoma (OPSCC), teeth with poor prognosis that pose a risk for post-RT osteoradionecrosis (ORN) are removed. The effect of tooth loss on body weight loss and tube feeding (TF) dependency during CRT/BRT is unknown. This study aimed to evaluate the effect of incomplete dentition, tooth extractions prior to CRT/BRT, and the subsequent loss of functional units on (1) weight loss during CRT/BRT and (2) the need for TF during CRT/BRT for OPSCC.

Methods
OPSCC patients treated with CRT/BRT between 2013 and 2016 were included in this retrospective cohort study. Dental status was determined during the dental assessment at first visit and after tooth extractions prior to the start of CRT/BRT. Weight loss during CRT/BRT was scored dichotomously, comparing weight loss > 5% to stable or increased weight. Potential factors associated with weight loss were identified, including patient, tumor, and treatment characteristics.

Results
Seventy-seven OPSCC patients were included. Forty patients (52%) experienced weight loss > 5% during CRT/BRT. Extractions were performed in 66% of the OPSCC patients. The mean number of extracted teeth was 4.1 ± 5.6 per patient. Tooth extractions prior to CRT/BRT were associated with weight loss > 5% during CRT/BRT (HR 1.130 (95% CI 1.011–1.262), p = 0.031). None of the dental status-related parameters showed any significant associative value for TF during CRT/BRT.

Conclusions
Pre-CRT/BRT tooth extractions intended to reduce the risk of ORN, are a risk factor for weight loss during CRT/BRT for OPSCC.

Trial registration number

This study was approved by the medical ethics committee of the MUMC + (METC 2020–1589) on July 28, 2020.
Electroanalytical point-of-care detection of gold standard and emerging cardiac biomarkers for stratification and monitoring in intensive care medicine - a review
Authors: Crapnell Robert D. , Dempsey Nina C. , Sigley Evelyn , Tridente Ascanio , Banks Craig E. 
Published: March 2022

AbstractDetermination of specific cardiac biomarkers (CBs) during the diagnosis and management of adverse cardiovascular events such as acute myocardial infarction (AMI) has become commonplace in emergency department (ED), cardiology and many other ward settings. Cardiac troponins (cTnT and cTnI) and natriuretic peptides (BNP and NT-pro-BNP) are the preferred biomarkers in clinical practice for the diagnostic workup of AMI, acute coronary syndrome (ACS) and other types of myocardial ischaemia and heart failure (HF), while the roles and possible clinical applications of several other potential biomarkers continue to be evaluated and are the subject of several comprehensive reviews. The requirement for rapid, repeated testing of a small number of CBs in ED and cardiology patients has led to the development of point-of-care (PoC) technology to circumvent the need for remote and lengthy testing procedures in the hospital pathology laboratories. Electroanalytical sensing platforms have the potential to meet these requirements. This review aims firstly to reflect on the potential benefits of rapid CB testing in critically ill patients, a very distinct cohort of patients with deranged baseline levels of CBs. We summarise their source and clinical relevance and are the first to report the required analytical ranges for such technology to be of value in this patient cohort. Secondly, we review the current electrochemical approaches, including its sub-variants such as photoelectrochemical and electrochemiluminescence, for the determination of important CBs highlighting the various strategies used, namely the use of micro- and nanomaterials, to maximise the sensitivities and selectivities of such approaches. Finally, we consider the challenges that must be overcome to allow for the commercialisation of this technology and transition into intensive care medicine.Graphical abstract
In silico prediction models for thyroid peroxidase inhibitors and their application to synthetic flavors
Authors: Seo Mihyun , Lim Changwon , Kwon Hoonjeong 
Published: March 2022

AbstractSystematic toxicity tests are often waived for the synthetic flavors as they are added in a very small amount in foods. However, their safety for some endpoints such as endocrine disruption should be concerned as they are likely to be active in low levels. In this case, structure–activity-relationship (SAR) models are good alternatives. In this study, therefore, binary, ternary, and quaternary prediction models were designed using simple or complex machine-learning methods. Overall, hard-voting classifiers outperformed other methods. The test scores for the best binary, ternary, and quaternary models were 0.6635, 0.5083, and 0.5217, respectively. Along with model development, some substructures including primary aromatic amine, (enol)ether, phenol, heterocyclic sulfur, and heterocyclic nitrogen, dominantly occurred in the most highly active compounds. The best predicting models were applied to synthetic flavors, and 22 agents appeared to have a strong inhibitory potential towards TPO activities.
Limited text speech synthesis with electroglottograph based on Bi-LSTM and modified Tacotron-2
Authors: Chen Lijiang , Ren Jie , Chen Pengfei , Mao Xia , Zhao Qi 
Published: March 2022

AbstractThis paper proposes a framework of applying only the EGG signal for speech synthesis in the limited categories of contents scenario. EGG is a sort of physiological signal which can reflect the trends of the vocal cord movement. Note that EGG’s different acquisition method contrasted with speech signals, we exploit its application in speech synthesis under the following two scenarios. (1) To synthesize speeches under high noise circumstances, where clean speech signals are unavailable. (2) To enable dumb people who retain vocal cord vibration to speak again. Our study consists of two stages, EGG to text and text to speech. The first is a text content recognition model based on Bi-LSTM, which converts each EGG signal sample into the corresponding text with a limited class of contents. This model achieves 91.12% accuracy on the validation set in a 20-class content recognition experiment. Then the second step synthesizes speeches with the corresponding text and the EGG signal. Based on modified Tacotron-2, our model gains the Mel cepstral distortion (MCD) of 5.877 and the mean opinion score (MOS) of 3.87, which is comparable with the state-of-the-art performance and achieves an improvement by 0.42 and a relatively smaller model size than the origin Tacotron-2. Considering to introduce the characteristics of speakers contained in EGG to the final synthesized speech, we put forward a fine-grained fundamental frequency modification method, which adjusts the fundamental frequency according to EGG signals and achieves a lower MCD of 5.781 and a higher MOS of 3.94 than that without modification.
A new insight into a thermoplastic microfluidic device aimed at improvement of oxygenation process and avoidance of shear stress during cell culture
Authors: Sheidaei Zohreh , Akbarzadeh Pooria , Nguyen Nam-Trung , Kashaninejad Navid 
Published: March 2022

AbstractKeeping the oxygen concentration at the desired physiological limits is a challenging task in cellular microfluidic devices. A good knowledge of affecting parameters would be helpful to control the oxygen delivery to cells. This study aims to provide a fundamental understanding of oxygenation process within a hydrogel-based microfluidic device considering simultaneous mass transfer, medium flow, and cellular consumption. For this purpose, the role of geometrical and hydrodynamic properties was numerically investigated. The results are in good agreement with both numerical and experimental data in the literature. The obtained results reveal that increasing the microchannel height delays the oxygen depletion in the absence of media flow. We also observed that increasing the medium flow rate increases the oxygen concentration in the device; however, it leads to high maximum shear stress. A novel pulsatile medium flow injection pattern is introduced to reduce detrimental effect of the applied shear stress on the cells.
Understanding the drivers of dispersal evolution in range expansions and their ecological consequences
Authors: Weiss-Lehman Christopher , Shaw Allison K. 
Published: March 2022

AbstractResearch has conclusively demonstrated the potential for dispersal evolution in range expansions and shifts, however the degree of dispersal evolution observed has varied substantially among organisms. Further, it is unknown how the factors influencing dispersal evolution might impact other ecological processes at play. We use an individual-based model to investigate the effects of the underlying genetics of dispersal and mode of reproduction in range expansions and shifts. Consistent with predictions from stationary populations, dispersal evolution increases with sexual reproduction and loci number. Contrary to our predictions, however, increased dispersal does not always improve a population’s ability to track changing conditions. The mate finding Allee effect inherent to sexual reproduction increases extinction risk during range shifts, counteracting the beneficial effect of increased dispersal evolution. Our results demonstrate the importance of considering both ecological and evolutionary processes for understanding range expansions and shifts.