bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative metabolomic profiling of women undergoing in vitro fertilization procedures reveals potential infertility-related biomarkers in follicular fluid
Authors: Dabaja Mohamed Ziad , dos Santos Aline Amaro , Christofolini Denise Maria , Barbosa Caio Parente , de Oliveira Diogo Noin , de Oliveira Arthur Noin , Melo Carlos Fernando Odir Rodrigues , Guerreiro Tatiane Melina , Catharino Rodrigo Ramos 
Published: November 2022

AbstractInfertility is a worldwide concern, affecting one in six couples throughout their reproductive period. Therefore, enhancing the clinical tools available to identify the causes of infertility may save time, money, and emotional distress for the involved parties. This study aims to annotate potential biomarkers in follicular fluid that are negatively affecting pregnancy outcomes in women suffering infertility-related diseases such as endometriosis, tuboperitoneal factor, uterine factor, and unexplained infertility, using a metabolomics approach through high-resolution mass spectrometry. Follicular fluid samples collected from women who have the abovementioned diseases and managed to become pregnant after in vitro fertilization procedures [control group (CT)] were metabolically compared with those from women who suffer from the same diseases and could not get pregnant after the same treatment [infertile group (IF)]. Mass spectrometry analysis indicated 10 statistically relevant differential metabolites in the IF group, including phosphatidic acids, phosphatidylethanolamines, phosphatidylcholines, phosphatidylinositol, glucosylceramides, and 1-hydroxyvitamin D3 3-d-glucopyranoside. These metabolites are associated with cell signaling, cell proliferation, inflammation, oncogenesis, and apoptosis, and linked to infertility problems. Our results indicate that understanding the IF’s metabolic profile may result in a faster and more assertive female infertility diagnosis, lowering the costs, and increasing the probability of a positive pregnancy outcome.
Cyanophages as an important factor in the early evolution of oxygenic photosynthesis
Authors: Ślesak Ireneusz , Ślesak Halina 
Published: November 2022

AbstractCyanophages are viruses that infect cyanobacteria. An interesting feature of many of them is the presence ofpsbAandpsbD, genes that encode D1 and D2 proteins, respectively. The D1 and D2 are core proteins of the photosystem II (PSII) in cyanobacteria, algae and plants and influence the proper function of oxygenic photosynthesis (OP) in all oxyphototrophs on Earth. The frequent occurrence ofpsbAandpsbDin cyanophages raises the question whether these genes coevolved with hosts during the early stages of cyanophage and cyanobacterial evolution, or whether they are direct descendants of genes adopted from the genomes of cyanobacterial hosts. The phylogeny of D1/D2 proteins encoded in the genomes of selected cyanophages and oxyphototrophs was reconstructed. In addition, common ancestral sequences of D1 and D2 proteins were predicted for cyanophages and oxyphototrophs. Based on this, the reconstruction of the 3D structures of D1 and D2 proteins was performed. In addition, the ratio of non-synonymous to synonymous (dN/dS) nucleotide substitutions in the coding sequences (CDSs) ofpsbAandpsbDwas determined. The results of the predicted spatial structures of the D1 and D2 proteins and purifying selection for the CDSs ofpsbAandpsbDsuggest that they belong to the ancient proteins, which may have formed the primordial PSII. It cannot be ruled out that they involved in water oxidation in cyanobacteria-like organisms at early stages of the evolution of life on Earth and coevolved with ancient cyanophages. The data are also discussed in the context of the origin of viruses.
Training with noninvasive brain–machine interface, tactile feedback, and locomotion to enhance neurological recovery in individuals with complete paraplegia: a randomized pilot study
Authors: Nicolelis Miguel A. L. , Alho Eduardo J. L. , Donati Ana R. C. , Yonamine Seidi , Aratanha Maria A. , Bao Guillaume , Campos Debora S. F. , Almeida Sabrina , Fischer Dora , Shokur Solaiman 
Published: November 2022

AbstractIn recent years, our group and others have reported multiple cases of consistent neurological recovery in people with spinal cord injury (SCI) following a protocol that integrates locomotion training with brain machine interfaces (BMI). The primary objective of this pilot study was to compare the neurological outcomes (motor, tactile, nociception, proprioception, and vibration) in both an intensive assisted locomotion training (LOC) and a neurorehabilitation protocol integrating assisted locomotion with a noninvasive brain–machine interface (L + BMI), virtual reality, and tactile feedback. We also investigated whether individuals with chronic-complete SCI could learn to perform leg motor imagery. We ran a parallel two-arm randomized pilot study; the experiments took place in São Paulo, Brazil. Eight adults sensorimotor-complete (AIS A) (all male) with chronic (> 6 months) traumatic spinal SCI participated in the protocol that was organized in two blocks of 14 weeks of training and an 8-week follow-up. The participants were allocated to either the LOC group (n = 4) or L + BMI group (n = 4) using block randomization (blinded outcome assessment). We show three important results: (i) locomotion training alone can induce some level of neurological recovery in sensorimotor-complete SCI, and (ii) the recovery rate is enhanced when such locomotion training is associated with BMI and tactile feedback (∆Mean Lower Extremity Motor score improvement for LOC =  + 2.5, L + B =  + 3.5; ∆Pinprick score: LOC =  + 3.75, L + B =  + 4.75 and ∆Tactile score LOC =  + 4.75, L + B =  + 9.5). (iii) Furthermore, we report that the BMI classifier accuracy was significantly above the chance level for all participants in L + B group. Our study shows potential for sensory and motor improvement in individuals with chronic complete SCI following a protocol with BMIs and locomotion therapy. We report no dropouts nor adverse events in both subgroups participating in the study, opening the possibility for a more definitive clinical trial with a larger cohort of people with SCI.Trial registration: http://www.ensaiosclinicos.gov.br/ identifier RBR-2pb8gq.
Chronic gut inflammation impairs contextual control of fear
Authors: Matisz C. E. , Patel M. , Hong N. S. , McDonald R. J. , Gruber A. J. 
Published: November 2022

AbstractChronic inflammatory diseases are highly comorbid with anxiety in humans. The extent to which chronic inflammation is responsible for this relationship remains to be determined. We therefore tested the hypothesis that prolonged, but not brief, gut inflammation is sufficient to evoke anxiety-related behaviours in mice. We used the discriminative fear to context conditioning paradigm to assess fear generalization, which is a prominent feature of anxiety disorders. Gut inflammation was induced by exposure to dextran sodium sulfate (DSS) in the drinking water, a well-established rodent model of ulcerative colitis evoking prolonged inflammation. Neither acute (1 × 5 day cycle) nor chronic (3 × 5 day cycles) exposure to DSS affected fear responses when tested shortly after conditioning. Mice in all groups generated more fear responses (freezing) in a chamber previously paired with mild shock, as compared to a chamber with no pairing. This suggests DSS exposure had no effect on acquisition or expression of conditioned fear. Acute and control animals showed this same contextual control of freezing when tested 9 days later. In contrast, at this remote time point, the chronically treated animals exhibited increased freezing in the unpaired chamber such that freezing was equivalent in both contexts. These animals, however, showed intact preference for the unpaired chamber when allowed to freely move between chambers. These data suggest that some mnemonic process engaged after training, such as memory consolidation, is affected by past chronic inflammation so as to generalize negative associations and engage fearful responding in inappropriate contexts, despite intact knowledge that the chambers have different affective associations sufficient for place preference.
Propagation characteristics and prediction of airblast overpressure outside tunnel: a case study
Authors: Zhou Xianshun , Zhang Xuemin , Wang Lichuan , Feng Han , Cai Chenzhi , Zeng Xiaohui , Ou Xuefeng 
Published: November 2022

AbstractThe drilling and blasting method is widely used in tunnel engineering. The accompanying airblast may damage structures and annoy nearby occupants. The prediction of airblast overpressure (poa) outside the tunnel is necessary to improve the safety of blasting works. A study of propagation characteristics of airblasts induced by tunnel blasting was carried out through experimental and numerical studies. The results indicate that the distributions of the poa outside the tunnel were anisotropic, which does not conform to the decay law of an explosion in free-field. The propagation of airblasts induced by tunnel blasting is related to the airblast shape. The phenomenon that the poa along the axial direction of the tunnel was higher than along other directions can be explained by the numerical results of the process of airblasts. The airblasts outside the tunnel traveled as a spherical wave, but the pressure was not uniformly distributed. After an airblast plane wave with high speed and high pressure inside the tunnel was transmitted out of the tunnel, its inertia strengthened the pressure in the axial direction. The airblast outside the tunnel is related to the propagation distance Rout, the angle from the measurement to the tunnel axis α, and the pressure intensity p0 at the tunnel portal. Subsequently, an ellipsoidal contour curve of the poa outside the tunnel was plotted, and therefore a new prediction equation was validated by numerical results and field data. Finally, the newly proposed methodology guided the blast design.
The effect of dietary halloysite supplementation on the performance and meat quality of pigs and some air indices in piggery
Authors: Nadziakiewicz Małgorzata , Lis Marcin Wojciech , Micek Piotr 
Published: November 2022

AbstractHalloysite, a clay mineral of the kaolin, has specific properties, characteristic for the conditions in which it was formed. The aim of the study was to determine the effect of halloysite from the Dunino deposit in a complete diet for pigs on daily body weight gain (BWG), feed conversion ratio (FCR), blood parameters, chemical composition of meat and chosen environment indices in the piggery. The trial was conducted on 144 piglets from weaning (d 29) to 85th day of life, then on 40 porkers divided into two groups. Animals were fed diets without (group C) or with halloysite (group E; 1.5% until d 128, then 1.0%). Pigs from group E were characterized by a lower number of days with diarrhea. The average BWG in E group was 44 g higher than in group C (P < 0.01). In turn, during the fattening period, the difference in BWG between groups was 60 g in favor of E (P < 0.05) and average FCR in group E was 4.9% lower compared to group C (P < 0.05). At the end of the study, the pigs from group E had 6.9% higher final body weight (P < 0.01). During both the morning and evening measurements, ammonia concentration in the air was lower by 16.3% and 23.8%, respectively. The use of halloysite enabled improvement of pig fattening efficiency, while reducing the costs of pork production and the negative effect of ammonia on the animals' welfare and environment.
Growth, yield, and yield variables of onion (Allium Cepa L.) varieties as influenced by plantspacing at DambiDollo, Western Ethiopia
Authors: Alemu Diribsa , Kitila Chala , Garedew Weyessa , Jule LetaTesfaye , Badassa Bayissa , Nagaprasad N. , Seenivasan Venkatesh , Saka Abel , Ramaswamy Krishnaraj 
Published: November 2022

AbstractOnion (Allium cepa L.) is an important bulb plant grown worldwide. Proper use of the agronomic practice has undoubtedly contributed to growing crop yields. The right level of any farming practice, like the distance between plants, plant density, date of planting, and time of harvest, can produce the wanted outcomes. Therefore, this research was piloted to evaluate the influence of plant spacing on the development of bulb harvest-related traits of onion varieties in Dambi Dollo University, Western Ethiopia, in 2021. Three onion varieties (Adama red, Monarch, Nafis) and four intra-row spaces (6 cm, 8 cm, 10 cm, and 12 cm) in factorial combinations were settled by a complete randomized block design which was simulated three times. The findings of the study indicate that all the factors related to crop growth and bulb yield of onion varieties were mainly influenced by different kinds and plant spacing. Conversely, the collaboration of these two factors did not affect all other factors, but the interaction of the two factors had a great effect on the days to maturity measured in this study. The highest plant height was registered on the onion planted at a distance of 10 cm (59.83 cm) and 12 cm (59.08 cm) distance between plants. The high commercial yields (34.44 t ha−1) and entire bulb yield (35.40 t ha−1) were found in the Nafis variety. The highest marketable yields (31.12 ha−1) and entire marketable yield (31.78 ha−1) were recorded on an onion plant planted 10 cm between plants. Therefore, in the research area, farmers can use a variety of Nafis and a 10 cm distance between plants to increase their onion production.
Mechanism of KIT gene regulation by GATA1 lacking the N-terminal domain in Down syndrome–related myeloid disorders
Authors: Kanezaki Rika , Toki Tsutomu , Terui Kiminori , Sato Tomohiko , Kobayashi Akie , Kudo Ko , Kamio Takuya , Sasaki Shinya , Kawaguchi Koji , Watanabe Kenichiro , Ito Etsuro 
Published: November 2022

AbstractChildren with Down syndrome (DS) are at high risk of transient abnormal myelopoiesis (TAM) and myeloid leukemia of DS (ML-DS). GATA1 mutations are detected in almost all TAM and ML-DS samples, with exclusive expression of short GATA1 protein (GATA1s) lacking the N-terminal domain (NTD). However, it remains to be clarified how GATA1s is involved with both disorders. Here, we established the K562 GATA1s (K562-G1s) clones expressing only GATA1s by CRISPR/Cas9 genome editing. The K562-G1s clones expressed KIT at significantly higher levels compared to the wild type of K562 (K562-WT). Chromatin immunoprecipitation studies identified the GATA1-bound regulatory sites upstream of KIT in K562-WT, K562-G1s clones and two ML-DS cell lines; KPAM1 and CMK11-5. Sonication-based chromosome conformation capture (3C) assay demonstrated that in K562-WT, the − 87 kb enhancer region of KIT was proximal to the − 115 kb, − 109 kb and + 1 kb region, while in a K562-G1s clone, CMK11-5 and primary TAM cells, the − 87 kb region was more proximal to the KIT transcriptional start site. These results suggest that the NTD of GATA1 is essential for proper genomic conformation and regulation of KIT gene expression, and that perturbation of this function might be involved in the pathogenesis of TAM and ML-DS.
Neural substrates underlying effortful control deficit in autism spectrum disorder: a meta-analysis of fMRI studies
Authors: Krishnamurthy Karthikeyan , Chan Melody M. Y. , Han Yvonne M. Y. 
Published: November 2022

AbstractEffortful control comprises attentional control, inhibitory control, and cognitive flexibility subprocesses. Effortful control is impaired in individuals with autism spectrum disorder, yet its neural underpinnings remain elusive. By conducting a coordinate-based meta-analysis, this study compared the brain activation patterns between autism and typically developing individuals and examined the effect of age on brain activation in each effortful control subprocesses. Meta-analytic results from 22 studies revealed that, individuals with autism showed hypoactivation in the default mode network for tasks tapping inhibitory control functioning (threshold-free cluster enhancement p < 0.001). When these individuals perform tasks tapping attentional control and cognitive flexibility, they exhibited aberrant activation in various brain networks including default mode network, dorsal attention, frontoparietal, visual and somatomotor networks (uncorrected ps < 0.005). Meta-regression analyses revealed that brain regions within the default mode network showed a significant decreasing trend in activation with increasing age (uncorrected p < 0.05). In summary, individuals with autism showed aberrant activation patterns across multiple brain functional networks during all cognitive tasks supporting effortful control, with some regions showing a decrease in activation with increasing age.
Toxicity assessment of potassium bromate and the remedial role of grape seed extract
Authors: Yalçin Emine , Çavuşoğlu Kültiğin 
Published: November 2022

AbstractIn this study, the multiple toxic effects of potassium bromate were investigated in Allium cepa L., an indicator test material. In addition, the toxicity-reducing effects of grape seed extract (GSE) were tested. The toxicity was investigated by some physiological (germination percentage, root length, weight gain, relative injury rate), cytogenetic [mitotic index (MI), micronucleus (MN), and chromosomal abnormalities (CAs)], biochemical [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) levels] and anatomical parameters. A. cepa bulbs were divided into 6 groups as control and five treatment groups (Group II: 465 mg/L GSE, Group III: 930 mg/L GSE, Group IV: 100 mg/L potassium bromate, Group V: 100 mg/L potassium bromate + 465 mg/L GSE, Group VI: 100 mg /L potassium bromate + 930 mg/L GSE). The bulbs were germinated for 72 h and at the end of the period the bulbs were subjected to routine preparations and made ready for analysis and measurements. As a result, potassium bromate exposure caused statistically significant (p < 0.05) decreases in all physiological parameter values. Potassium bromate application decreased MI by 41.6%, and increased the MN and CAs frequencies. CAs such as fragment, sticky chromosome, and vagrant chromosome, unequal distribution of chromatin, reverse polarization, nuclear bud and disordered mitosis were induced in root meristem cells. The mechanism of potassium bromate genotoxicity has been associated with DNA-potassium bromate interaction supported by spectral shift. Potassium bromate caused a decrease in GSH levels and an increase in MDA, SOD and CAT levels, thereby disrupting the antioxidant/oxidant balance in root tip cells. GSE administration in two different doses together with potassium bromate reduced the toxic effects and caused improvements in all parameters examined. The most significant reduction in toxicity was in group VI, which received 930 mg/L GSE, and there was an improvement about 18% in MI levels and an improvement about 44% in GSH levels in this group. While GSE application increased physiological parameters and GSH levels, it decreased MDA, SOD, CAT levels, MN and CAs frequencies. As a result, it has been determined that potassium bromate causes multi-directional toxicity at high doses and A. cepa is a very reliable indicator in determining this toxicity. In addition, GSE extract has been found to have a strong role in reducing the toxicity induced by potassium bromate.
Comprehensive examination of radiative electromagnetic flowing of nanofluids with viscous dissipation effect over a vertical accelerated plate
Authors: Bejawada Shankar Goud , Reddy Yanala Dharmendar , Jamshed Wasim , Usman  , Isa Siti Suzilliana Putri Mohamed , El Din Sayed M. , Guedri Kamel , Rehman M. Israr Ur 
Published: November 2022

AbstractThis research aims to establish the MHD radiating convective nanofluid flow properties with the viscous dissipation across an exponentially accelerating vertical plate. As the plate accelerates, its temperature progressively increases. There are two separate types of water-based nanofluids that include copper ($$Cu$$

Cu

) and titanium dioxide ($$Ti{O}_{2}$$

T
i

O
2

) nanoparticles, respectively. The most crucial aspect of this investigation is finding a closed-form solution to a nonlinear coupled partial differential equations scheme. Galerkin finite element method (G-FEM) is used to figure out the initial managing equations. Utilizing graphs, the effect of the flow phenomenon's contributing variables as well as the influence of other factors is determined and depicted. In the part dedicated to the findings and discussion, the properties of these emergent parameters are described in more depth. Nonetheless, the thermal radiation and heat sink factors increase the thermal profile. In addition, the greater density of the copper nanoparticles cause the nanoparticle volume fraction to lessen the velocity delineation.
Sampling investigation and statistical analysis for mental problems of freshmen in Shandong Province
Authors: Li Chang , Sun Bingchuan 
Published: November 2022

Abstractniversity stage, especially the freshmen stage, is a high incidence stage of students’ psychological problems. Effective sampling investigation and statistical analysis of freshmen’s mental health problems are conducive to solve freshmen’s problems and prevent further crises. In the past 4 years (2018–2021), using Chinese college students’ mental health screening scale, we have taken probability proportionate to size sampling investigations about the mental problems to 9882 freshmen in 45 public universities in Shandong Province near the end of their first semester. Based on these data, we conducted the comparison of anxiety and depression for post pandemic era vs. pre pandemic era by analysis of variance, and analysed the influencing factors for anxiety and depression by linear regression and canonical correlation analysis. The results indicate the extents of anxiety and depression in post pandemic era are significantly more severe than pre pandemic era. Inferiority complex, obsession and somatization are the main effect factors of anxiety and depression. To the best of our knowledge, this research is the first systematical investigation and analysis for the mental problems among freshmen in the whole Shandong Province before and after the epidemic. The research results are conducive for the mental health counseling and intervention of freshmen’s mental problems, and also helpful for policy making and prevention for psychological crisis management.
The influence of anxiety and fear of COVID-19 on vaccination hesitancy among postsecondary students
Authors: Šorgo Andrej , Crnkovič Nuša , Cesar Katarina , Selak Špela , Vrdelja Mitja , Gabrovec Branko 
Published: November 2022

AbstractThe aim of the present study was to explore the influence of anxiety and fear of COVID-19 on vaccination hesitancy among Slovenian postsecondary students. A cross-sectional study using a set of previously tested instruments and ad hoc questions created by the authors was chosen as the method to gain insight into various health and sociodemographic aspects of Slovenian postsecondary students affected by the COVID-19-induced closures and suspensions of educational activities at tertiary educational institutions (N = 5999). Overall, 39.7% of participating students expressed an intention to get vaccinated at the first possible opportunity, whereas 29.2% expressed no intent to do so. The highest vaccine hesitancy was observed among prospective teachers (50.3%) and the lowest among prospective physicians (5,7%). When examining the role of anxiety and fear of COVID-19 on the Slovenian postsecondary students’ intentions to get vaccinated the results of logistic regression showed that only fear of COVID-19 played a mild and significant role.