bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic Analysis of Cuproptosis-related Genes in Gastric Cancer
Authors: Kong Fanhua , Wang Kunpang , Teng Chuang 
Published: October 2022

Abstract
Background
Gastric cancer (GC) is a serious malignant tumor with high morbidity and mortality and poor prognosis worldwide. Cuproptosis is a new type of cell death that can induce proteotoxic stress and ultimately lead to cell death, which is associated with tumor progression, prognosis and immune response. In this study, the expression of cuproptosis-related genes (CRGs) was analyzed to predict the prognosis of GC patients.
Methods
We analyzed the expression and mutation status of CRGs in 407 GC patients from TCGA database and 433 GC patients from GEO database, and correlated them with clinical prognosis. The R software package was used for classification. The relationship between different groups and prognosis, risk genes and immune microenvironment was further analyzed. LASSO cox algorithm was used to construct a cuproptosis risk model according to 8 risk genes. Finally, we constructed nomogram and calibration curve to predict the survival probability of patients and performed antitumor drug sensitivity analysis.
Results
Based on the analysis of TCGA and GEO databases, there were significant differences in the expression level and prognosis of CRGs in GC. We used consensus clustering algorithm to classify CRGs, and found 2 clusters of CRGs characterized by immune cell infiltration, and obtained 195 differentially expressed genes. We further obtained 8 risk genes by multivariate Cox regression analysis and constructed a cuproptosis risk model. Receiver operating characteristic curve (ROC) and principal component analysis (PCA) show that the model has accurate prediction ability. Risk score is an independent prognostic factor for GC patients. In addition, patients with low CRGs score have higher tumor mutation burden and immune activation level, and better survival prognosis. However, patients with high CRGs score showed poor survival and immunosuppression.
Conclusion
CRGs are involved in the occurrence and development of GC. Our cuproptosis risk model provides a new research strategy for predicting the prognosis of GC patients. Meanwhile, the results of drug sensitivity analysis can provide valuable drug candidate clues for clinical treatment of GC.
The multi-year effect of different agroecological practices on soil nematode and soil respiration
Authors: Sun Feng , Coulibaly Sekou FM , Cheviron Nathalie , Mougin Christian , Hedde Mickael , Maron Pierre-Alain , Recous Sylvie , Trap Jean , Villenave Cécile , Chauvat Matthieu 
Published: October 2022

Abstract
Background and Aims:
Agroecology practices can induce profound changes in soil inevitably inﬂuencing soil biological properties and soil functioning. However, we still lack understanding of how soil biodiversity responds to agroecology practices and to what extent such practices, alone or combined, can be beneficial for soil functioning. Understanding soil biological activities under different agroecology practices is important for predicting carbon cycling in agroecosystems.
Methods:
By taking advantage of a long-term agricultural experimental research station in France, we monitored soil microbes, nematodes and soil respiration over three years in response to agroecology practices that varied in the rate of nitrogen (N) fertilization (low vs high), the tillage type (deep vs reduced), and the crop residue management (retain vs removal).
Results:
Shifting from conventional to agroecology practices had strong effects on microbial biomass, nematode community and soil respiration. Reduced N and reduced tillage increased microbial biomass carbon, bacterivore and fungivore density. Perennial biomass crop decreased total nematode and herbivore density, but increased microbial biomass. Perennial biomass crop also significantly increased the structure and maturity indices, but decreased the plant parasite indices. Structural equation modelling showed that microbial biomass had a positive correlation with soil respiration in reduced nitrogen, reduced tillage, and residue removal treatments. Bacterivores had a positive correlation with omnivores/predators and soil respiration, while herbivores had a negative correlation with soil respiration in all the treatments.
Conclusions:
The different agroecological practices tested in this 4-year trial revealed the resilience of nematode communities and associated functions like CO2 respiration according to practices.
Endometrial giant cell carcinoma: a case report and review of the literature
Authors: Tang Xiao , Li Lei , Jiang Wei 
Published: October 2022

Abstract
Background

Endometrial giant cell carcinoma (EGCC) is a rare variant of endometrial carcinoma, and only two small series and few case reports have been described in the literatures. It has not been recognized as a separate entity in the current WHO classification of endometrial neoplasms. Data on the biological behavior, clinical management, and prognosis of EGCC are limited due to its rarity.

Case presentation

A 55-year-old postmenopausal woman presented with vaginal bleeding for about 2 months. Transvaginal ultrasound examination showed an echogenic polypoid mass measuring 1.8cm×1.2cm×1.6cm in the uterine cavity, a hysteroscopic lesion resection was performed. Histologically, in the background of multifocal necrosis, the tumor was remarkably composed of a large number of multinucleated giant cells and mononucleated cells, and a small part (10%) of the conventional EC components which exhibited mostly as serous carcinoma and focally as endometrioid differentiation scattered among them. In immunohistochemical staining, the tumor cells were strongly positive for EMA, P53(aberrant nuclear expression), Vimentin, molecular testing demonstrated p53-mutant subtype for molecular classification of EC. The pathological diagnosis was EGCC accompanied with serous carcinoma. The patient underwent a total abdominal hysterectomy, bilateral salpingo-oophorectomy and pelvic lymphadenectomy. The patient did not receive postoperative radiochemotherapy, and no evidence of disease was found after follow-up for 1 year.

Conclusion

EGCC is a rare type of uterine malignant tumor. It is necessary to realize this entity may be a rare variant of endometrial carcinoma, and the presence and/or percentage of giant cell component should be clearly notated in the pathology report, indicating that the biological behavior is unclear. Further experience for diagnose and treatment of this rare tumor is warranted.
Cartel Leniency and Settlements: A Joint Perspective
Authors: Dijkstra Peter T. , Seifert Jacob 
Published: October 2022

Abstract
This paper studies the interactions between a cartel leniency program and a settlement procedure. The EU settlement procedure gives colluding firms that do not apply for leniency an additional opportunity to cooperate with the competition authority in exchange for a reduced fine once a preliminary case has been established against them. We derive the conditions under which colluding firms apply for leniency, settle, or refuse to cooperate with the authority in equilibrium. Our policy results show that settlements can act as a complement or a substitute to the leniency program. We also study the welfare-optimal policy and highlight a novel interdependence between the fine reductions that should be offered to leniency applicants and settling firms.
JEL classification K21 · L31 · L41
The road of &gt;1000 corpses: landscape and road-related features that promote mortality in the Amazon
Authors: Medrano-Vizcaíno Pablo , Grilo Clara , Brito-Zapata David , González-Suárez Manuela 
Published: October 2022

Abstract
Roads impact wildlife around the world; however, dedicated studies are lacking in many biodiverse areas such as the Amazon. Identifying which species are more often hit by vehicles and which landscape and road-related features promote roadkill is essential to guide future development and ensure adequate mitigation actions. For six months, we monitored 240 km of roads in the Ecuadorian Amazon and recorded 1125 dead vertebrates (148 species). Reptiles were the most affected class with 380 individuals (56 species), followed by amphibians with 278 individuals (11 species), birds with 259 individuals (62 species), and mammals with 208 individuals (20 species). We used Random Forest models to explore the role of various land cover types and road sinuosity on the observed mortality. Additionally, we created heatmaps to visualize the road segments where roadkills were more frequent. For all vertebrates, mortality was more likely in straight road sections near rivers. The effect of other variables was taxa-specific. Amphibian mortality was more likely near bare soil or forest, birds and mammals died more often near herbaceous-shrubby vegetation, whereas reptile mortality occurred more often further from herbaceous-shrubby vegetation. Road segments with a high mortality (roadkill hotspots) varied across taxa. These hotspots identify areas where further research is needed to assess road impacts and where mitigation could prevent collisions. Among records, we found rare and threatened species, including some that may be new to science. Roadkill surveys not only aid in quantifying threats and informing future planning but can also provide insight into local biodiversity.
Crop-climate link in the southeastern USA: A case study on Oats and Sorghum
Authors: Sharma Ramandeep Kumar , Dhillon Jagmandeep , Kumar Sunny , Vatta Kamal , Reddy Krishna N. 
Published: October 2022

Abstract
Recognizing the crop and region-specific irreversible effects of climate change on agriculture is unavoidable. The Southeastern United States region (SE-US) contributes significantly to the United States (US) economy through its diverse agricultural productivity. Climatically, this region is more vulnerable than the rest of the country. This study was designed to quantify the effect of changing climate, i.e., daily maximum temperature (Tmax), daily minimum temperature (Tmin), and precipitation, on oats (Avena sativa L.) and sorghum (Sorghum bicolor L. Moench) in SE-US. The panel data approach with a fixed effects model was applied by creating a production function on a panel dataset (1980–2020) of climate and yield variables. The required diagnostic tests were used to statistically confirm that the dataset was free of multi-collinearity, stationarity, and auto-correlation issues. The results revealed asymmetric warmings (Tmin increase > Tmax increase) over the region. Tmax and Tmin significantly increased during the oats growing season (OGS) and sorghum growing season (SGS). Precipitation increased during OGS and decreased during SGS. The annual average values of Tmax, Tmin, and Tavg (daily average temperature) have shifted by 1.08°C (0.027°C/year), 1.32°C (0.033°C/year), and 1.20°C (0.030°C/year) in OGS and by 0.92°C (0.023°C/year), 1.32°C (0.033°C/year), and 1.12°C (0.028°C/year) in SGS. However, precipitation had shifted by 23.2 mm (0.58 mm/year) in OGS and shifted (decreased) by -5.2 mm (-0.13 mm/year) in SGS. Precipitation had a non-significant effect on oats and sorghum yields. With every 1°C increase in Tmin and Tmax, oats yield was reduced by (-5%) and (-4%), respectively, whereas sorghum yield was increased by (+ 13%) and decreased by (-7%), respectively. Taken together, a 1°C net rise in overall temperature reduced oats yield (-9%) while increased sorghum yield (+ 6%).
The Ocular Manifestations of Relapsing Polychondritis
Authors: Gallagher Kevin , Al-Janabi Ahmed , Wang Aijing 
Published: October 2022

AbstractPurposeRelapsing polychondritis (RPC) is a rare, multi-system, inflammatory disorder. Ocular disease is estimated to occur in 14 to 67% of patients with RPC and any ocular structure can be affected. Published case reports and series of RPC were analysed to determine the frequency and nature of the ocular manifestations of RPC, including the age and gender distribution.MethodsA literature search of the MEDLINE database for case reports and series on RPC was conducted using search terms (relapsing polychondritis[MeSH Major Topic]) OR (relapsing polychondritis[Title/Abstract]). Articles were included if the diagnosis of RPC was confirmed using established diagnostic criteria and if the paper described the clinical features of patients with RPC.Results546 articles (454 case reports and 92 case series) described the clinical features in a total of 2414 patients with RPC. 49% of patients with RPC had ocular involvement and this was a presenting feature in 21%. The most common ocular manifestations were scleritis (32%), episcleritis (31%) and uveitis (23%).ConclusionMany patients with RPC will be seen by an ophthalmologist during the course of their disease. Knowledge and awareness of RPC and its ocular manifestations is therefore essential to enable the ophthalmologist to make the diagnosis.
Bi-enzyme photoelectrochemical biosensor for sensitive determination of phosphatidylcholine in vegetable oil
Authors: Song Yang , Sha Jincheng , Wang Cuntang , Liu Xinyue , Han Lixin , Li Li 
Published: October 2022

Abstract
A bi-enzyme PEC biosensor was explored for the sensitive determination of phosphatidylcholine (PC) in vegetable oil based on phospholipase D (PLD) and choline oxidase (ChOx) immobilized on an indium tin oxide (ITO) electrode. The bi-enzyme electrode is based on modifying SnO2 nanoparticles (SnO2 NPs) and polythionine (PTh). SnO2 NPs were obtained by hydrothermal method. The electropolymerization was performed to load the PTh layer on the ITO/SnO2 NPs electrode surface. The immobilization method of PLD and ChOx combined embedding and cross-linking. This bi-enzyme PEC biosensor with ITO/SnO2 NPs/PTh/ChOx/PLD electrode was developed for PC detection. Under the optimized conditions, a good linear relationship was obtained in the range of 0.01 mM to 5 mM with a detection limit of 0.002 (S/N=3). The proposed PEC analysis method shows good reproducibility, selectivity, and stability. The prepared PEC biosensor was used to detect PC in vegetable oil, and the results were consistent with those of liquid chromatography. This is an effective PEC sensing strategy for the rapid and sensitive determination of PC in vegetable oil.
Time Series Analysis of Environmental Data by using ARIMA and LSTM models for Precision Agriculture
Authors: Khanna Abhishek , Kaur Sanmeet 
Published: October 2022

Abstract
Appropriate utilization of water and its distribution within the fields for irrigation purpose within the agricultural domain is of paramount importance. It not only helps in retaining the natural resource, but also eliminates various environmental risks associated, such as crop damage, soil fertility loss, etc. This estimation is can be predicted in advance after evaluating all the associated agricultural parameters mainly soil moisture, humidity, luminosity, and atmospheric pressure thoroughly. Farmers often practice water distribution within the fields without evaluating its precise requirement. With an aim to procure this natural resource and evaluate its distribution as per the requirement of the fields, the present work endorses evaluation of agricultural parameters through Autoregressive Integrated Moving Average (ARIMA) and Long short-term memory (LSTM) models based on Time Series analysis. Both these models predicts the values based on following two moving average methods, Simple Moving Average (SMA) and Exponential Moving Average (EMA). For obtaining values related to agricultural parameters, Libelium's Waspmote Plug & Sense, a hardware device has been incorporated within the study. The evaluated results depicted variation in results, in contrast to the actual practices begin followed by farmers. The intermediate diminution in error rates attained by ARIMA were found within the range of 74% - 77% in contrast to LSTM, indicating its preeminence. Based on the existing results, future possible values for various attributes can be estimated and on the basis of estimation, distribution of water for irrigating the fields can be scheduled according to requirement, resulting in better fields yields.
Misdiagnosis of Cardiac Manifestations of Seronegative Spondyloarthritis as Congenital Mitral Valve Apparatus Malformation by Echocardiography: A Case Report
Authors: Liu Shengnan , han Shu , Ma ChunYan , Yang Jun 
Published: October 2022

Abstract
Ankylosing spondylitis is the prototypical form of the seronegative spondyloarthropathies. Approximately 2–10% of patients have cardiac involvement. We reported a case of cardiac involvement of ankylosing spondylitis. An accurate imaging identification and differentiation is important for treatment decisions-making. Some nonspecific or uncommon symptoms of cardiac involvement were outstanding such that other departments clinicians excluded or overlooked it. Hence, the misdiagnosis rate of the disease is rather high, especially for cardiac involvement.
Tribological characteristics of three-body abrasive wear on MoS2 films
Authors: CHENG Weilun , dong shaojiang 
Published: October 2022

Abstract

Aiming at the problem of the failure of bearing lubrication caused by the entry of fine dust into the spacecraft, a molecular dynamic simulation method was proposed to study the wear characteristics of the bearing lubricating film. Firstly, the atomic model of MoS2 thin film is established, and the contact state of dust particles on the surface of MoS2 thin film is divided (two-body contact state and three-body contact state). Secondly, the characteristics of two-body and three-body abrasive wear are studied by applying different loads to the abrasives. Finally, the surface structure of the substrate was changed to explore the adsorption and wear characteristics of the one-dimensional rough substrate. The following conclusions are obtained: 1) Two-body contact and three-body contact have different wear mechanisms. The wear of two-body contact originates from the accumulation and release of tangential force. The main source of wear in three-body contact is the release of tangential force and the action of tangential force caused by rolling; 2) The relationship between sliding friction and rolling friction at the atomic scale is the same as that at the macroscopic scale. Rolling friction is much less friction than sliding friction. Rolling friction causes less damage to the film than sliding friction; 3) The one-dimensional rough substrate reduces the contact area between the film and the film, resulting in a decrease in the adsorption between the film and the substrate. The asperity structure of rough substrates reduces the lubricity of the film compared to smooth substrates.
SIGIRR-caspase-8 signaling mediates endothelial apoptosis in Kawasaki disease
Authors: wen zhengwang , Xia Yuhan , Zhang Yingying , He Yuxi , Niu Chao , Wu Rongzhou , zhang chunxiang , jia chang , Rong Xing , Chu Maoping 
Published: October 2022

Abstract
Background: Kawasaki disease (KD) is a kind of vasculitis with unidentified etiology. Given that the current diagnosis and therapeutic strategy of KD are mainly dependent on clinical experiences, further research to explore its pathological mechanisms is warranted.
Methods: Enzyme linked immunosorbent assay (ELISA) was used to measure the serum levels of SIGIRR, TLR4 and caspase-8. Western blotting was applied to determine protein levels, and flow cytometry was utilized to analyze cell apoptosis. Hematoxylin eosin (HE) staining and TUNEL staining were respectively used to observe coronary artery inflammation and DNA fragmentation.
Results: in this study, we found the level of SIGIRR was downregulated in KD serum and KD serum-treated endothelial cells. However, the level of caspase-8 was increased in serum from KD patients compared with healthy control (HC). Therefore, we hypothesized that SIGIRR-caspase-8 signaling may play an essential role in KD pathophysiology. In vitro experiments demonstrated that endothelial cell apoptosis in the setting of KD was associated with caspase-8 activation, and SIGIRR overexpression alleviated endothelial cell apoptosis via inhibiting caspase-8 activation. These findings were also recapitulated in the Candida albicans cell wall extracts (CAWS)-induced KD mouse model.
Conclusion: our data suggest that endothelial cell apoptosis mediated by SIGIRR-caspase-8 signaling plays a crucial role in coronary endothelial damage, providing potential targets to treat KD.
The Auditory Brainstem Response of the Chicken Hatchling
Authors: Ordiway George , McDonnell Miranda , Sanchez Jason Tait 
Published: October 2022

AbstractThe auditory brainstem response (ABR) is a useful approach for evaluating hearing function. It is easily acquired in vertebrate animal models like reptiles, birds, and mammals, and routinely utilized in clinical audiology with human patients. The ABR is complementary in animal models with existing developmental andin vitroresearch. However, despite the domestic white leghorn chicken (gallus gallus domesticus) being studied extensively at the molecular, cellular, and system levels for decades, there exists no modern characterization of the chicken hatchling ABR. To address this gap, we used click- and tone-burst stimuli to elicit the ABR in 43 wild type chicken hatchlings one- and two-days post hatch (P1 and P2). Click-evoked ABRs presented with three to four positive going microvolt (µV) peaks within 6 milliseconds (ms) post stimulus onset. Tone-evoked ABRs resembled findings in other avian species; thresholds from 100–4000 Hertz (Hz) exhibited a range of best sensitivity between 750–2000 Hz. Population latency/intensity functions revealed an increase in peak latency of roughly 300 microseconds (µs) per a decrease of 20 decibels sound pressure level (dB SPL) for both click- and tone-evoked ABRs. The chicken hatchling ABR not only complements existing molecular, cellular, and systems level research, but also resembles the ABR waveform of altricial avian species, highlighting the precocious development of the chicken.
Comparison of National-Level Cybersecurity and Cyber Power Indices: A Conceptual Framework
Authors: Çifci Hasan 
Published: October 2022

Abstract
Cybersecurity and cyber power have grown in importance as a way of mitigating risks and threats and maintaining a secure and reliable cyberspace. Several methodologies have been proposed to measure the cybersecurity and cyber power of nation states from different perspectives. In this study, the global indices and studies for assessing cybersecurity and cyber power were analyzed and compared in terms of their comprehensiveness and strength for measuring country-level capabilities. A conceptual framework covering all of the indicators of well-known indices was developed for comparison, and it can also be used as a new model to measure cyber capabilities at a national level. This study is unique in terms of the number and diversity of the indices as well as the scope and depth of the comparison since it analyzed most of the well-known country-level cybersecurity and cyber power indices as well as a maturity model at the indicator level depth. The study provides an extensive list of the categories and indicators and thereby improves comprehension of the elements of cybersecurity and cyber power with the comparison and mappings of widely used indices.