bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychometric and factorial validity of the International Adjustment Disorder Questionnaire (IADQ) in an Italian sample: A validation and prevalence estimate study
Authors: Jannini Tommaso B. , Rossi Rodolfo , Socci Valentina , Reda Flaminia , Pacitti Francesca , Di Lorenzo Giorgio 
Published: December 2022

AbstractIntroductionAdjustment disorder (AjD) is a is a maladaptive emotional or behavioural reaction to a stressful event or change in a person's life. Compared to other previously validated tools, the International Adjustment Disorder Questionnaire (IADQ) stands out as one of the most reliable and handy one for AjD.Since no homologous instrument exists now, in this study, we aimed to validate an Italian version of the IADQ.MethodsTwenty‐one thousand two hundred and six subjects (80.4% females) during the initial stages of the SARS‐CoV‐2 pandemic were recruited. We conducted a confirmatory factor analysis (CFA), testing two latent models, a monofactorial and a bifactorial one. Concurrent validity by correlating the total and the two factors' scores with measures of depression, anxiety and post‐traumatic stress was then estimated. We finally estimated the rates of AjD among the population, and a binary logistic regression was conducted to analyse the predictors of such disorder.ResultsCFA showed a bifactorial validity, with both excellent incremental and comparative fit indices. The IADQ scores correlated strongly with symptoms of depression, anxiety and stress. In the Italian sample, the prevalence of probable AjD was 8.23%. Female gender, being engaged, widowed and having COVID‐19‐related stressors resulted as significant independent risk factors for AjD.ConclusionsIADQ is an easy‐to‐use, brief and psychometrically sound self‐report measure for AjD. Thus, it may be considered a reliable tool for both research and clinical settings. To the best of our knowledge, our study reported for the first time the prevalence of AjD during COVID‐19 pandemic.
The moderating and mediating role of telepresence and cognitive change in cognitive behaviour therapy delivered via videoconference
Authors: Bouchard Stéphane , Berthiaume Maxine , Robillard Geneviève , Allard Micheline , Green‐Demers Isabelle , Watts Stéphanie , Marchand André , Gosselin Patrick , Langlois Frédéric , Belleville Geneviève , Dugas Michel J. 
Published: December 2022

AbstractIn this study, we combined the results of two controlled trials and examined the relationships between working alliance, telepresence, cognitive change and treatment outcome. Sixty‐five participants with a primary diagnosis of generalized anxiety disorder (GAD) or panic disorder with agoraphobia (PDA) received cognitive behaviour therapy delivered via videoconference. Participants completed measures of working alliance and telepresence after three psychotherapy sessions. They also completed measures of treatment outcome and dysfunctional beliefs (cognitive change) specific to PDA and GAD at pretreatment and posttreatment. Results revealed that telepresence at the fifth session moderated the relationship between the working alliance at the first and fifth sessions. As telepresence increased, its impact on the working alliance diminished. Cognitive change mediated the relationship between the working alliance at the fifth session and treatment outcome.
Chromium (VI)‐induced ALDH1A1/EGF axis promotes lung cancer progression
Authors: Ge Xin , Li Mengdie , Song Guo‐Xin , Zhang Zhixiang , Yin Jianxing , Ge Zehe , Shi Zhumei , Liu Ling‐Zhi , Jiang Bing‐Hua , Qian Xu , Shen Hua 
Published: December 2022

AbstractCr(VI) is broadly applied in industry. Cr(VI) exposure places a big burden on public health, thereby increasing the risk of lung squamous cell carcinoma (LUSC). The mechanisms underlying Cr(VI)‐induced LUSC remain largely elusive. Here, we report that the cancer stem cell (CSC)/tumour‐initiating cell (TIC)‐like subgroup within Cr(VI)‐transformed bronchial epithelial cells (CrT) promotes lung cancer tumourigenesis. Mechanistically, Cr(VI) exposure specifically increases the expression levels of aldehyde dehydrogenase 1A1 (ALDH1A1), a CSC marker, through KLF4‐mediated transcription. ALDH1A1 maintains self‐renewal of CrT/TICs and facilitates the expression and secretion of EGF from CrT/TICs, which subsequently promotes the activation of EGFR signalling in differentiated cancer cells and tumour growth of LUSC. In addition, the ALDH1A1 inhibitor A37 and gemcitabine synergistically suppress LUSC progression. Importantly, high ALDH1A1 expression levels are positively correlated with advanced clinical stages and predict poor survival in LUSC patients. These findings elucidate how ALDH1A1 modulates EGF secretion from TICs to facilitate LUSC tumourigenesis, highlighting new therapeutic strategies for malignant lung cancers.
From JWH‐018 to OXIZIDS: Structural evolution of synthetic cannabinoids in the European Union from 2008 to present day
Authors: Andrews Rachael , Jorge Rita , Christie Rachel , Gallegos Ana 
Published: December 2022

AbstractWith new synthetic cannabinoids (SCs) appearing on the European drug market every year, early warning systems are key to detect, monitor, and respond to threats posed by them. The European Union Early Warning System (EU EWS) implemented by the European Monitoring Centre for Drugs and Drug Addiction has monitored these substances since their first European detection in 2008. Since then, national and international responses have been implemented, aimed at tackling risks posed by SCs. Throughout this time, new SCs have emerged on the European market containing diverse structural moieties, appearing to be designed in a way that circumvents existing legal controls, contributing to a complex public health scenario. This study provides an inventory of the SCs detected in the EU from 2008 to 2022, describing their structural evolution by analysing separately four structural features: their core, tail, linker, and linked groups. The range of structural changes is analysed considering key milestones, including the year of first report by the European Union Early Warning System to the key legislative changes that have occurred since. The analysis shows that from June 2021 to July 2022, 20 out of 23 newly emerged SCs evade the generic SC legislation introduced in China in May 2021. This supports the hypothesis that the protection of public health benefits from timely information exchange and careful assessment of the risks associated with these substances. Additionally, the introduction of legal responses, albeit an important instrument to reduce the availability of dangerous substances on the market, may also be accompanied by unintended consequences.
A systematic review comparing atypical anorexia nervosa and anorexia nervosa
Authors: Walsh B. Timothy , Hagan Kelsey E. , Lockwood Carlin 
Published: December 2022

AbstractObjectiveA description of atypical anorexia nervosa (atypical AN) was provided in DSM‐5 in 2013 and a sizable literature has since developed describing the clinical features of individuals with atypical AN and comparing them to those of individuals with anorexia nervosa (AN) and those of healthy individuals. The purpose of this study was to conduct a systematic review of this literature.MethodA systematic review following PRISMA guidelines was conducted of studies published since 2013 that compared the clinical characteristics of individuals with atypical AN to those of individuals with AN and/or healthy controls. Meta‐analyses were conducted when similar measures were reported in three or more studies.ResultsTwenty‐four publications met criteria for inclusion. Their results indicated that the level of eating disorder‐specific psychopathology is significantly higher among individuals with atypical AN than among controls and as high or higher as among individuals with AN while levels of non‐eating disorder psychopathology are similar. Individuals with atypical AN experience many of the physiological complications associated with AN, but some complications appear less frequent.DiscussionThe psychological symptoms and physiological complications of individuals with atypical AN are generally similar to those of individuals with AN, although there may be differences in the frequency of some physical complications. Little information is available on the course, outcome, and treatment response of individuals with atypical AN. In addition, full diagnostic criteria for atypical AN have not been developed, and the nosological relationship of atypical AN to established eating disorders such as bulimia nervosa is unclear.Public SignificanceAtypical anorexia nervosa as described in the DSM‐5 identifies individuals with many of the psychological characteristics of typical anorexia nervosa who, despite significant weight loss, are not underweight. The current systematic review found that the psychological symptoms and physiological characteristics of individuals with atypical AN are generally similar to those of individuals with AN, although there may be differences in the frequency of some physical complications.
COL4A1 mutation‐related disorder presenting as fetal intracranial bleeding, hydrocephalus, and polymicrogyria
Authors: Straka Barbora , Vlčková Markéta , Libá Zuzana , Heřmanovská Barbora , Kynčl Martin , Dorňáková Jana , Táborský Jakub , Kršek Pavel , Musilová Alena , Janota Jan , Balaščaková Miroslava 
Published: December 2022

AbstractFetal intracranial hemorrhage represents a rare event with an estimated prevalence of 1:10 000 pregnancies. We report a patient diagnosed prenatally with intracranial hemorrhage and ventriculomegaly carrying a novel, previously unreported, likely pathogenic variant in COL4A1. At the gestational age of 27 weeks, dilation of lateral ventricles was detected during a routine prenatal ultrasound scan, confirmed by prenatal MRI at 30 + 3 weeks of gestation. Prenatal examinations included amniocentesis with conventional G‐band karyotyping and arrayCGH, and maternal testing for TORCH and parvovirus B19 infections. Virtual gene panel based on whole‐exome sequencing data was performed postnatally. At the age of 2.5 months, the patient manifested epileptic seizures that remain difficult to control. Postnatal MRI showed partial thalamic fusion and polymicrogyria, in addition to severe enlargement of lateral ventricles, multiple deposits of hemosiderin in cerebral and cerebellar hemispheres, and thin optic nerve and chiasma. Virtual gene panel based on whole‐exome sequencing data led to a detection of a de novo previously unreported in‐frame deletion NM_001845.5:c.4688_4711del in COL4A1 located in the highly conserved NC1 domain initiating collagen helix assembly. The presented case lies one a more severe end of the COL4A1 mutation‐related disease spectrum, manifesting as fetal intracranial bleeding, malformation of cortical development, drug‐resistant epilepsy, and developmental delay.
A case of FN1‐fused calcified chondroid mesenchymal neoplasm of the hand with novel FGFR3 partner gene
Authors: Georgantzoglou Natalia , Shen Guomiao , Jour George , Linos Konstantinos 
Published: December 2022

AbstractCalcified chondroid neoplasms with FN1::FGFR1 or FGFR2 fusions constitute a recently described category of mesenchymal neoplasms mostly encountered in the extremities and temporomandibular joint. Herein, we report a case of FNI1‐fused calcified chondroid neoplasm of the hand with a novel FGFR3 fusion partner. The tumor exhibited a multilobulated growth pattern composed of epithelioid cells embedded in abundant stroma with myxoid, chondroid, and fibrous areas and scattered osteoclast‐like giant cells. RNA sequencing revealed an in‐frame fusion between Exon 31 of FN1 and Exon 3 of FGFR3, which was subsequently confirmed by reverse transcription‐polymerase chain reaction. Our findings expand on the spectrum of potential fusion partners in FN1‐fused calcified chondroid neoplasms.
Multiyear methane and nitrous oxide emissions in different irrigation management under long‐term continuous rice rotation in Arkansas
Authors: Karki S. , Adviento‐Borbe M. A. A. , Runkle B. R. K. , Moreno‐García B. , Anders M. , Reba M. L. 
Published: December 2022

AbstractRice paddies are one of the major sources of anthropogenic methane (CH4) emissions. The alternate wetting and drying (AWD) irrigation management has been shown to reduce CH4 emissions and total global warming potential (GWP) (CH4 and nitrous oxide [N2O]). However, there is limited information about utilizing AWD management to reduce greenhouse gas (GHG) emissions from commercial‐scale continuous rice fields. This study was conducted for five consecutive growing seasons (2015–2019) on a pair of adjacent fields in a commercial farm in Arkansas under long‐term continuous rice rotation irrigated with either continuously flooded (CF) or AWD conditions. The cumulative CH4 emissions in the growing season across the two fields and 5 years ranged from 41 to 123 kg CH4‐C ha−1 for CF and 1 to 73 kg CH4‐C ha−1 for AWD. On average, AWD reduced CH4 emissions by 73% relative to CH4 emissions in CF fields. Compared to N2O emissions, CH4 emissions dominated the GWP with an average contribution of 91% in both irrigation treatments. There was no significant variation in grain yield (7.3–11.9 Mg ha−1) or growing season N2O emissions (−0.02 to 0.51 kg N2O‐N ha−1) between the irrigation treatments. The yield‐scaled GWP was 368 and 173 kg CO2 eq. Mg−1 season−1 for CF and AWD, respectively, showing the feasibility of AWD on a commercial farm to reduce the total GHG emissions while sustaining grain yield. Seasonal variations of GHG emissions observed within fields showed total GHG emissions were predominantly influenced by weather (precipitation) and crop and irrigation management. The influence of air temperature and floodwater heights on GHG emissions had high degree of variability among years and fields. These findings demonstrate that the use of multiyear GHG emission datasets could better capture variability of GHG emissions associated with rice production and could improve field verification of GHG emission models and scaling factors for commercial rice farms.
Is pomegranate husk scald during storage induced by water loss and mediated by ABA signaling?
Authors: Maghoumi Mahshad , Fatchurrahman Danial , Amodio Maria Luisa , Quinto Maurizio , Cisneros‐Zevallos Luis , Colelli Giancarlo 
Published: December 2022

AbstractBackgroundHusk scald (HS) is a physiological disorder limiting the marketability of pomegranate fruit during long‐term storage. Herein we propose that HS is triggered by water loss and mediated by ABA signaling. Therefore, pomegranate fruit were stored at three different storage temperatures (3.5, 7 and 11°C) and 96.5% ± 2.3% relative humidity (RH) evaluating weight loss (WL), abscisic acid (ABA), respiration rate (RR), total phenolics (TP), total anthocyanin (TA), antioxidant activity (AA), exocarp electrolyte leakage (EL), malondialdehyde (MDA), color attributes, browning index and visual quality of fruit.ResultsHS appeared after 3 months of storage at 11 °C, less at 7°C and non‐present at 3.5°C. Incidence of HS occurred along with higher WL, RR, EL, MDA, and ABA content. Conversely, TP, TA and AA decreased significantly. WL increased with water vapor pressure deficit (VPD) at higher temperatures. After 93 days, 11 °C fruit reached ~10% WL while ABA increased to ~150 μg kg−1. However, 3.5 and 7 °C fruit, reached 5 and 7% WL while ABA was ~31 and 75 μg kg−1, respectively.ConclusionHerein, we propose a mechanistic model of HS development where water stress induces ABA as a primary signaling molecule that triggers the HS response mediated by reactive oxygen species (ROS). Accumulation of ROS induces phenolic biosynthesis and oxidative stress promotes loss of membrane compartmentalization that induces phenolic degradation. Ultimately, husk scalding becomes visible due to the oxidation of phenolics into brown pigments. © 2022 Society of Chemical Industry.
Biotin‐Functionalized Block Catiomers as an Active Targeting Approach in Gene Delivery
Authors: Pretzer Irene , Bushiri David , Weberskirch Ralf 
Published: December 2022

AbstractRecently, biotin (vitamin H) has been described as a ligand for active targeting and it has been found that many cancer cells overexpress the biotin receptor. In this study a biotin‐conjugated block copolymer, biotin‐poly(2‐ethyl‐2‐oxazoline)‐block‐poly{N '‐[N‐(2‐aminoethyl)‐2‐aminoethyl]aspartamide} (biotin‐pEtOx‐b‐pASP(DEA) is synthesized by a living cationic polymerization of the pEtOx‐block followed by the nucleophilic ringopening polymerization of the pASP‐block. The biotin moiety is coupled to the pEtOx‐b‐pASP precursor by a Cu(I) mediated azide‐alkyne click chemistry and finally, the diethylamine (DEA) side chain is introduced by a polymer analogous reaction. The final polymer P1 formed polyplexes in the presence of plasmid DNA that are characterized with respect to N/P ratio, size, zeta potential, and shape compared to a control polymer P2 without biotin. In addition, HEK293 cells are transfected with these polyplexes and the number of fluorescent HEK293 cells is evaluated to assess the influence of polymer nature on the activity of the micelles. Flow cytometric analysis revealed a significantly higher uptake of the biotin‐PEtOx‐PASP(DEA)/pDNA micelle than the PEtOx‐PASP(DEA)/pDNA micelle against HEK293 cells at a low N/P ratio of 20, consistent with the transfection results whereas at higher N/P ratio no difference can be observed anymore between the two polymers.
Evanescent‐mode bandpass filter using D‐shaped slotlines for spurious harmonic suppression
Authors: Chen Peng , Kai Yang , Shu Yue , Liu Zheng 
Published: December 2022

AbstractA novel miniaturized evanescent‐mode substrate integrated waveguide (SIW) bandpass filter with D‐shaped slotlines for spurious harmonic suppression is proposed in this letter. Closed‐form equations for the proposed evanescent‐mode SIW cavity are summarized and two D‐shaped slotlines are etched together as a pair by the face‐to‐face style to obtain the designed lowpass feature, which could be utilized to improve the out‐of‐band spurious harmonic rejection level. To validate the feasibility of this method, a miniaturized evanescent‐mode SIW filter with D‐shaped slotlines is designed and measured. The measured results show that this filter operates at 1.05 GHz with 2.4 dB insertion loss and 3.8% bandwidth. The out‐of‐band spurious rejection is lower than 16 dB up to 11 times of the higher cutoff frequency of the passband. All test results are in good agreement with the simulation results.
Commissioning a beam line for MR‐guided particle therapy assisted by in silico methods
Authors: Fuchs Hermann , Padilla‐Cabal Fatima , Oborn Bradley M. , Georg Dietmar 
Published: December 2022

AbstractBackgroundRadiation therapy is continuously moving towards more precise dose delivery. The combination of online MR imaging and particle therapy, for example, radiation therapy using protons or carbon ions, could enable the next level of precision in radiotherapy. In particle therapy, research towards a combination of MR and particle therapy is well underway, but still far from clinical systems. The combination of high magnetic fields with particle therapy delivery poses several challenges for treatment planning, treatment workflow, dose delivery, and dosimetry.PurposeTo present a workflow for commissioning of a light ion beam line with an integrated dipole magnet to perform MR‐guided particle therapy (MRgPT) research, producing not only basic beam data but also magnetic field maps for accurate dose calculation. Accurate dose calculation in magnetic field environments requires high‐quality magnetic field maps to compensate for magnetic‐field‐dependent trajectory changes and dose perturbations.MethodsThe research beam line at MedAustron was coupled with a resistive dipole magnet positioned at the isocenter. Beam data were measured for proton and carbon ions with and without an applied magnetic field of 1 T. Laterally integrated depth‐dose curves (IDC) as well as beam profiles were measured in water while beam trajectories were measured in air. Based on manufacturer data, an in silico model of the magnet was created, allowing to extract high‐quality 3D magnetic field data. An existing GATE/Geant4 Monte Carlo (MC) model of the beam line was extended with the generated magnetic field data and benchmarked against experimental data.ResultsA 3D magnetic field volume covering fringe fields until 50 mT was found to be sufficient for an accurate beam trajectory modeling. The effect on particle range retraction was found to be 2.3 and 0.3 mm for protons and carbon ions, respectively. Measured lateral beam offsets in water agreed within 0.4 and −0.5 mm with MC simulations for protons and carbon ions, respectively. Experimentally determined in‐air beam trajectories agreed within 0.4 mm in the homogeneous magnetic field area.ConclusionThe presented approach based on in silico modeling and measurements allows to commission a beam line for MRgPT while providing benchmarking data for the magnetic field modeling, required for state‐of‐the art dose calculation methods.
Improving precision and power in randomized trials with a two‐stage study design: Stratification using clustering method
Authors: Ye Xuan , Lu Nelson , Xu Yunling 
Published: December 2022

AbstractIn a randomized controlled trial (RCT), it is possible to improve precision and power and reduce sample size by appropriately adjusting for baseline covariates. There are multiple statistical methods to adjust for prognostic baseline covariates, such as an ANCOVA method. In this paper, we propose a clustering‐based stratification method for adjusting for the prognostic baseline covariates. Clusters (strata) are formed only based on prognostic baseline covariates, not outcome data nor treatment assignment. Therefore, the clustering procedure can be completed prior to the availability of outcome data. The treatment effect is estimated in each cluster, and the overall treatment effect is derived by combining all cluster‐specific treatment effect estimates. The proposed implementation of the procedure is described. Simulations studies and an example are presented.
Viral modulation of lipid rafts and their potential as putative antiviral targets
Authors: Gee Yee Jing , Sea Yi Lin , Lal Sunil Kumar 
Published: December 2022

AbstractLipid rafts are ubiquitous in cells. They are identified as cholesterol and glycosphingolipid enriched microdomains on cellular membranes. They serve as platforms for cellular communications by functioning in signal transduction and membrane trafficking. Such structural organisation fulfils cellular needs for normal function, but at the same time increases vulnerability of cells to pathogen invasion. Viruses rely heavily on lipid rafts in basically every stage of the viral life cycle for successful infection. Various mechanisms of lipid rafts modification exploited by diverse viruses for attachment, internalisation, membrane fusion, genome replication, assembly and release have been brought to light. This review focuses on virus‐raft interactions and how a wide range of viruses manipulate lipid rafts at distinct stages of infection. The importance of virus‐raft interactions in viral infections has inspired researchers to discover and develop antivirals that target this interaction, such as statins, methyl‐β‐cyclodextrin, viperin, 25‐hydroxycholesterol and even anti‐malarial drugs. The therapeutic modulations of lipid rafts as potential antiviral intervention from in vitro and in vivo evidence are discussed herein.