bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuron matters: neuromodulation with electromagnetic stimulation must consider neurons as dynamic identities
Authors: Ye Hui , Hendee Jenna , Ruan Joyce , Zhirova Alena , Ye Jayden , Dima Maria 
Published: November 2022

AbstractNeuromodulation with electromagnetic stimulation is widely used for the control of abnormal neural activity, and has been proven to be a valuable alternative to pharmacological tools for the treatment of many neurological diseases. Tremendous efforts have been focused on the design of the stimulation apparatus (i.e., electrodes and magnetic coils) that delivers the electric current to the neural tissue, and the optimization of the stimulation parameters. Less attention has been given to the complicated, dynamic properties of the neurons, and their context-dependent impact on the stimulation effects. This review focuses on the neuronal factors that influence the outcomes of electromagnetic stimulation in neuromodulation. Evidence from multiple levels (tissue, cellular, and single ion channel) are reviewed. Properties of the neural elements and their dynamic changes play a significant role in the outcome of electromagnetic stimulation. This angle of understanding yields a comprehensive perspective of neural activity during electrical neuromodulation, and provides insights in the design and development of novel stimulation technology.
Age-related radiographic parameters difference between the degenerative lumbar spinal stenosis patients and healthy people and correlation analysis
Authors: Li Donglai , Wang Lianlei , Wang Zheng , Li Chao , Yuan Suomao , Tian Yonghao , Yu Xuguang , Liu Xinyu 
Published: November 2022

Abstract
Objectives
To identify age-related radiographic risk factors for degenerative lumbar spinal stenosis (DLSS) and analyze correlations among them.

Methods
A total of 180 cases were enrolled in this study, and lumbar magnetic resonance was performed. Among them, 93 cases suffered DLSS and lumbar dynamic X-ray was examined. And following parameters were measured and evaluated: intervertebral disk height (IDH), the ratio of IDH(IDHL4-5/L3-4), initial IDH of L4-5(iIDHL4-5) in the DLSS group, disk degeneration (DD), cartilaginous endplate failure (CEF), Modic changes, the thickness of ligamentum flavum (LF), range of intervertebral motion (ROM), facet joint opening (FJO), facet joint angle (FJA), the standard cross-sectional area (SCSA) of the multifidus, erector spinae, and psoas major muscles. The data of two groups were compared, and the possible risk factors of DLSS were analyzed.

Results
Compared with the control group, the DLSS group had higher IDH except for L4-5 and larger iIDHL4-5 (P < 0.05). Significant differences were shown in CEF and the thickness of LF at L1-S1 and DD at L4-5 (P < 0.05). The DLSS group had smaller SCSA of multifidus, erector spinae, and psoas major muscles but greater FJA, FJO (P < 0.05). And the risk of DLSS increased when iIDHL4-5 ≥ 10.73 mm, FJA ≥ 52.03° , or FJO ≥ 3.75 mm. IDH positively correlated with SCSA of multifidus and psoas major muscles and ROM at L1-S1 (P < 0.05). DD showed negative linear relations with SCSA of multifidus and psoas muscle and positive linear relation with CEF at L1-2, L2-3, and L5-S1 (P < 0.05).

Conclusion
Larger initial disk height and excessive CEF may induce DLSS by increasing intervertebral mobility to promote DD, and atrophied paravertebral muscles by weakening the stability of lumbar spine.
A new proof of evidence of cysteamine quantification for therapeutic drug monitoring in patients with cystinosis
Authors: Franzin Martina , Rossetto Silvia , Ruoso Rachele , Del Savio Rossella , Stocco Gabriele , Decorti Giuliana , Addobbati Riccardo 
Published: November 2022

Abstract
Background
To date, measurement of intracellular cystine is used for the therapeutic monitoring of patients affected by cystinosis in treatment with cysteamine. Since this method is time and sample consuming, development of a faster method to quantify cysteamine would be extremely useful in order to help clinicians to adjust dosages of cysteamine and to define better the pharmacokinetic profile of this drug. The aim of the study was to develop a liquid chromatography tandem mass spectrometry method for the quantification of cysteamine in plasma samples and to test its applicability on plasma samples derived from patients with nephropathic infantile cystinosis in treatment with cysteamine.

Results
The percentage of accuracy of the developed method varied between 97.80 and 106.00% and CV% between 0.90 and 6.93%. There was no carry over. The calibration curves were built from 2.5 to 50 µM. The limit of detection and the lower limit of quantification occurred at 0.25 and 1.25 µM respectively. Cysteamine was stable up to 2 months at -20 °C. Concentrations of cysteamine and intracellular cystine of 4 patients were in line with data previously reported.

Conclusion
The proposed method showed an appropriate selectivity, specificity, linearity, sensibility, accuracy, precision and good applicability to samples.
Whole blood methylome-derived features to discriminate endocrine hypertension
Authors: Armignacco Roberta , Reel Parminder S. , Reel Smarti , Jouinot Anne , Septier Amandine , Gaspar Cassandra , Perlemoine Karine , Larsen Casper K. , Bouys Lucas , Braun Leah , Riester Anna , Kroiss Matthias , Bonnet-Serrano Fidéline , Amar Laurence , Blanchard Anne , Gimenez-Roqueplo Anne-Paule , Prejbisz Aleksander , Januszewicz Andrzej , Dobrowolski Piotr , Davies Eleanor , MacKenzie Scott M. , Rossi Gian Paolo , Lenzini Livia , Ceccato Filippo , Scaroni Carla , Mulatero Paolo , Williams Tracy A. , Pecori Alessio , Monticone Silvia , Beuschlein Felix , Reincke Martin , Zennaro Maria-Christina , Bertherat Jérôme , Jefferson Emily , Assié Guillaume 
Published: November 2022

Abstract
Background
Arterial hypertension represents a worldwide health burden and a major risk factor for cardiovascular morbidity and mortality. Hypertension can be primary (primary hypertension, PHT), or secondary to endocrine disorders (endocrine hypertension, EHT), such as Cushing's syndrome (CS), primary aldosteronism (PA), and pheochromocytoma/paraganglioma (PPGL). Diagnosis of EHT is currently based on hormone assays. Efficient detection remains challenging, but is crucial to properly orientate patients for diagnostic confirmation and specific treatment. More accurate biomarkers would help in the diagnostic pathway. We hypothesized that each type of endocrine hypertension could be associated with a specific blood DNA methylation signature, which could be used for disease discrimination. To identify such markers, we aimed at exploring the methylome profiles in a cohort of 255 patients with hypertension, either PHT (n = 42) or EHT (n = 213), and at identifying specific discriminating signatures using machine learning approaches.

Results
Unsupervised classification of samples showed discrimination of PHT from EHT. CS patients clustered separately from all other patients, whereas PA and PPGL showed an overall overlap. Global methylation was decreased in the CS group compared to PHT. Supervised comparison with PHT identified differentially methylated CpG sites for each type of endocrine hypertension, showing a diffuse genomic location. Among the most differentially methylated genes, FKBP5 was identified in the CS group. Using four different machine learning methods—Lasso (Least Absolute Shrinkage and Selection Operator), Logistic Regression, Random Forest, and Support Vector Machine—predictive models for each type of endocrine hypertension were built on training cohorts (80% of samples for each hypertension type) and estimated on validation cohorts (20% of samples for each hypertension type). Balanced accuracies ranged from 0.55 to 0.74 for predicting EHT, 0.85 to 0.95 for predicting CS, 0.66 to 0.88 for predicting PA, and 0.70 to 0.83 for predicting PPGL.

Conclusions
The blood DNA methylome can discriminate endocrine hypertension, with methylation signatures for each type of endocrine disorder.
Rare presentation of Waldenström’s macroglobulinaemia requiring bilateral above-knee amputations: a case report
Authors: Deen Raeed , Austin Calyb , Bowden Alexander , Bullen Andrew 
Published: November 2022

Abstract
Background
Waldenström’s macroglobulinaemia is a rarely encountered B-lymphocytic malignancy. Waldenström’s macroglobulinaemia-associated paraproteinaemia is linked to an increase in serum viscosity, which results in a hypercoagulable state. Burning bilateral foot pain in a man with alcohol dependence and controlled atrial fibrillation presenting to the emergency department was attributed to peripheral neuropathy, given satisfactory angiographic evidence of bilateral foot arterial blood supply. Subsequently, his presentation as an emergency with acute bilateral critical lower limb ischemia that was managed by bilateral above-knee amputations, prompted a wider search for other etiologies. We present a hitherto unreported case of Waldenström’s macroglobulinaemia-related acute bilateral lower limb ischemia, which required bilateral above-knee amputations.

Case presentation
A 50-year-old Caucasian man, who was an alcohol dependent heavy smoker, presented with burning pain in his right foot that was deemed to be related to alcoholic neuropathy. A computerized tomographic angiogram demonstrated an occluded right distal anterior tibial artery but a patent posterior tibial artery supplying the foot arch, findings that were associated with noncritical ischemia. After multiple presentations within a week, he was admitted following sudden clinical deterioration with acute confusion, hyponatremia, and bilateral foot pain. Over the course of 24 hours, the patient deteriorated rapidly, with bilateral lower limb ischemia requiring bilateral above-knee amputations. Subsequent investigations revealed a diagnosis of Waldenström’s macroglobulinaemia.

Conclusions
To the best of our knowledge, this is the only reported case of Waldenström’s macroglobulinaemia-induced bilateral lower limb ischemia requiring major bilateral amputations.
Psoriatic arthritis successfully treated with second-line anti-interleukin-6 treatment: a case report and review of the literature
Authors: Kutsuna Tatsuhiko , Hino Kazunori , Hasegawa Hitoshi , Watamori Kunihiko , Kidani Teruki , Imai Hiroshi , Miura Hiromasa 
Published: November 2022

Abstract
Background
Psoriatic arthritis treatment with antitumor necrosis factor has been shown to reduce disease activity. Nonetheless, more than 30% of patients do not achieve a sufficient response to tumor necrosis factor blockers. Currently, treatment with interleukin-6 inhibitors is expected to be effective and suppress the joint destruction in patients with psoriatic arthritis; however, evidence regarding their efficacy is limited to a few reports.

Case presentation
A 78-year-old Japanese woman with psoriatic arthritis associated with rapid joint destruction was successfully treated with a second-line anti-interleukin-6 receptor agent. In this case, a tumor necrosis factor inhibitor induced an inadequate response, and the right knee and left hip joints required artificial joint replacement surgery. However, second line treatment with anti-interleukin-6 treatment was effective, and the right elbow joint function was preserved.

Conclusions
We experienced a case of psoriatic arthritis, in which anti-interleukin-6 treatment repaired a bone cyst in the lateral epicondyle of the humerus and enthesitis of the distal interphalangeal joints. The patient is currently in clinical remission with no restrictions in daily life activities. Anti-interleukin-6 treatment may address the unmet needs of patients with psoriatic arthritis who are resistant or intolerant to antitumor necrosis factor treatment, with rapidly destructive large joints but with well-managed skin manifestations.
Biocatalytic production of the antibiotic aurachin D in Escherichia coli
Authors: Kruth Sebastian , Schibajew Lina , Nett Markus 
Published: November 2022

Abstract
Aurachin D is a potent inhibitor of cytochrome bd oxidases, which are potential targets in the treatment of infectious diseases. In this study, our aim was to improve the biocatalytic production of aurachin D from a quinolone precursor molecule with recombinant Escherichia coli cells expressing the biosynthesis enzyme AuaA. In order to achieve a high-level production of this membrane-bound farnesyltransferase in E. coli, the expression of the auaA gene was translationally coupled to an upstream cistron in accordance with a bicistronic design (BCD) strategy. Screening of various BCD elements led to the identification of optimized auaA expression cassettes, which increased the aurachin D titer in E. coli up to 29-fold in comparison to T7-mediated expression. This titer could be further raised by codon optimization of auaA and by introducing the mevalonate pathway into the production strain. The latter measure was intended to improve the availability of farnesyl pyrophosphate, which is needed as a cosubstrate for the AuaA-catalyzed reaction. In sum, the described efforts resulted in a strain producing aurachin D with a titer that is 424 times higher than that obtained with the original, non-optimized expression host.

Graphical Abstract
Reconnoitering the significance of security using multiple cloud environments for conveyance applications with blowfish algorithm
Authors: Shitharth S , Alotaibi Fahad S. , Manoharan Hariprasath , Khadidos Adil O. , Alyoubi Khaled H. , Alshareef Abdulrhman M. 
Published: November 2022

AbstractIn recent years the process of transportation needs a highly effective traffic system in order to monitor all consumer goods as many goods are left out at different locations. To handle such moving cases cloud platform is highly helpful as with respect to geographical location the goods are mapped in correct form. However incorporation of single cloud platform does not provide sufficient amount of storage about all goods thus a multiple cloud platform is introduced in proposed system. As multiple cloud platform is provided the security features of each data base system is also checked and enhanced using encryption keys. Moreover for proper operating conditions of multiple cloud platforms an analytical model is designed that synchronizes necessary data at end system. The defined analytical model focuses on solving multiple objectives that are related to critical energy problems where demand problems are reduced. Further the encryption process is carried out using Improved BlowFish Algorithm (IBFA) by allocating proper resources with decryption keys. To validate the effectiveness of proposed method five scenarios are considered where all scenario outcomes proves to be much higher than existing models by an average of 43%.
Biochemical and histopathological studies of sulfonylurea derivative as a new chemotherapeutic agent against liver cancer in free- and nano-coated forms
Authors: Sroor Farid M. , Basyouni Wahid M. , Aly Hanan F. , Younis Eman A. , Mahrous Karima F. , Haroun Ahmed A. 
Published: November 2022

AbstractThe most frequent type of primary liver cancer is hepatocellular carcinoma (HCC), accounting for approximately 90% of primary liver cancers and a third leading cause of cancer deaths. In the current study, the synthesized compound 3 was re-formulated using tetraethyl orthosilicate (TEOS) with weight ratio (1:1) via sol-gel technique. The prepared material has been examined using Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray elemental analysis (EDX), and scanning and transmission electron microscopes (SEM and TEM). Herein, we investigate the mode of action of 3 as potent anti-liver cancer in vivo as normal and nano-forms. Rats were given a single dosage of 50 mg/kg b.wt. of HCC through an intraperitoneal injection (ip). A single dosage of CCl4 (2 ml/kg IP) was also given to rats 2 weeks later. Several liver, tumor and oxidative stress biomarkers were detected including liver enzymes; alanine and aspartate aminotransferases (ALT and AST), alkaline phosphatse (ALP), gamma glutamyl transferase (GGT), glutathione (GSH), lipid peroxide (MDA), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (TAC), α-fetoprotein and α-L-Fucosidase. Hepatic pathological pictures were also performed for the documentation of the presence of HCC and supported the biochemical results. Moreover, the DNA damage in liver tissues of male rats using comet assay was studied. The results showed that the HePG2 (− ve) group of rats exhibited a significant reduction (P < 0.05) in DNA damage values (9.30 ± 0.89) relative to other treatment groups. Nevertheless, the DNA damage values in the HePG2 (+ ve) and 5-flurouracil groups were significantly higher (P < 0.01) compared to the HePG2 (− ve) group. Additionally, HePG2 (coated 3) and HePG2 (3) groups exhibited significant decrease in the DNA damage compared to those in HePG2 (+ ve) group.
Early intubation and clinical outcomes in patients with severe COVID-19: a systematic review and meta-analysis
Authors: Lee Hyeon-Jeong , Kim Joohae , Choi Miyoung , Choi Won-Il , Joh Joonsung , Park Jungeun , Kim Junghyun 
Published: November 2022

Abstract
Background
Evidence regarding the timing of the application of mechanical ventilation among patients with severe coronavirus disease (COVID-19) is insufficient. This systematic review and meta-analysis aimed to evaluate the effectiveness of early intubation compared to late intubation in patients with severe and critical COVID-19.

Methods
For this study, we searched the MEDLINE, EMBASE, and Cochrane databases as well as one Korean domestic database on July 15, 2021. We updated the search monthly from September 10, 2021 to February 10, 2022. Studies that compared early intubation with late intubation in patients with severe COVID-19 were eligible for inclusion. Relative risk (RR) and mean difference (MD) were calculated as measures of effect using the random-effects model for the pooled estimates of in-hospital mortality, intensive care unit (ICU) length of stay (LOS), duration of mechanical ventilation (MV), hospital LOS, ICU-free days, and ventilator-free days. Subgroup analysis was performed based on the definition of early intubation and the index time. To assess the risk of bias in the included studies, we used the Risk of Bias Assessment tool for Non-randomized studies 2.0.

Results
Of the 1523 records identified, 12 cohort studies, involving 2843 patients with severe COVID-19 were eligible. There were no differences in in-hospital mortality (8 studies, n = 795; RR 0.91, 95% CI 0.75–1.10, P = 0.32, I2 = 33%), LOS in the ICU (9 studies, n = 978; MD −1.77 days, 95% CI −4.61 to 1.07 days, P = 0.22, I2 = 78%), MV duration (9 studies, n = 1,066; MD −0.03 day, 95% CI −1.79 to 1.72 days, P = 0.97, I2 = 49%), ICU-free days (1 study, n = 32; 0 day vs. 0 day; P = 0.39), and ventilator-free days (4 studies, n = 344; MD 0.94 day, 95% CI −4.56 to 6.43 days, P = 0.74, I2 = 54%) between the early and late intubation groups. However, the early intubation group had significant advantage in terms of hospital LOS (6 studies, n = 738; MD −4.32 days, 95% CI −7.20 to −1.44 days, P = 0.003, I2 = 45%).

Conclusion
This study showed no significant difference in both primary and secondary outcomes between the early intubation and late intubation groups.
Trial registration This study was registered in the Prospective Register of Systematic Reviews on 16 February, 2022 (registration number CRD42022311122).
Application of different foliar iron fertilizers for improving the photosynthesis and tuber quality of potato (Solanum tuberosum L.) and enhancing iron biofortification
Authors: Zhang Ruyan , Zhang Weina , Kang Yichen , Shi Mingfu , Yang Xinyu , Li Hong , Yu Huifang , Wang Yong , Qin Shuhao 
Published: November 2022

Abstract
Background
The potato (Solanum tuberosum L.) is an important food crop in the alkaline soil area of northwest China. It is abundant in ascorbic acid (vitamin C), which facilitates iron absorption in the body. The large consumption of potato makes it a good food source of iron absorption for human body. However, iron deficiency in alkaline soil regions reduced chlorophyll synthesis in the leaves, resulting in lower photosynthesis and less sugar supply to the plant's healthy organs, significantly restricted crop development and yield, and inflicted major economic losses.

Methods
In this study, a 2-year (2020–2021) field trial was designed. Under foliar application of five different iron fertilizers: ferric sulfate [(Fe2(SO4)3], T1; ferrous sulfate (FeSO4·7H2O), T2; citric acid/ferric sulfate (CA/Fe2(SO4)3), T3; citric acid/ferrous sulfate (CA/FeSO4·7H2O), T4; ethylenediamine tetra acetic acid (EDTA-Fe·Na), T5, changes in potato plant photosynthesis were compared to no iron fertilization, CK conditions. The effects of various iron fertilizers on the yield, quality, and iron content of potato tubers, their correlations to chlorophyll levels, and the characteristics of photosynthetic fluorescence were studied.

Results
The results indicated that spraying iron fertilizers increased the yield, quality and Fe content of the tubers, which might be due to the improvement of the plants’ photosynthetic pigment content, gas exchange parameters and chlorophyll fluorescence. In two consecutive years of cultivation, we found that potato tuber yield and Fe content increased in potatoes treated with five iron fertilizer sprays. Among them, tuber yield increased most significantly by T5 and T3 treatments compared to CK, while Fe content was significantly higher by the T5 treatment than by CK and other treatments. Tuber yield was increased by 33.28% and 18.85% in 2020 and 50.74% and 54.48% in 2021 by T5 and T3 treatments, respectively, compared to CK. Fe content was increased by 112.64% and 54.98% in 2020 and 2021 by T5 treatment, respectively, compared to CK.

Conclusions
EDTA-Fe·Na and CA/Fe2(SO4)3 excelled over the other iron fertilizers. The findings of this study are instructive for developing cost-effective iron fertilizer management systems to maximize the impact of iron biofortification on human health.

Graphical Abstract
Wheat and chaff: the degree to which strategic management principles are integrated within corporate social responsibility reporting among large Canadian firms
Authors: Fuller Mark 
Published: November 2022

AbstractThis empirical study examines the degree to which strategic principles are reflected in the corporate social responsibility (CSR) reporting practices among Canada’s largest corporations. In a two-phased approach, three time periods of corporate disclosure from 2016 to 2020 were studied. Using an organizational flowchart derived from the literature, CSR disclosures were organized according to six degrees of strategic integration. Analysis reveals a bimodal distribution of firms, with one mode representing firms with a fully integrated reporting framework, and another mode representing firms with the partial integration of strategic management principles. Inconsistent patterns of progress in reporting practices were discovered, with some firms demonstrating improved reporting practices while others stagnated or declined. Overall, a generalized improvement in strategic integration during our multi-year examination was observed. While this work has implications for corporate social responsibility theory, recommendations for CSR practitioners are also discussed.