bluuebunny/crossref_metadata_2025_split huggingface dataset:
CLONING, PURIFICATION AND STUDY OF THE BIOCHEMICAL PROPERTIES OF Α-AMYLASE FROM BACILLUS LICHENIFORMIS T5 STRAIN
Authors: Kiribayeva A.K  , Silayev D.V  , Tursunbekova A.E  , Ramankulov Y.M  , Khassenov B.В  
Published: March 2022

Amylases are rather important enzymes in modern biotechnology. Alpha-amylases hydrolyze starch to form glucose and maltose and are involved in the conversion of starch-containing raw materials. In comparison with α-amylases of eukaryotic organisms, bacterial α-amylases have a number of advantages, namely, the preservation of enzymatic activity at high temperatures, which is important in enzymatic starch hydrolysis technologies. In this work, the α-amylase gene from the Kazakh strain of Bacillus licheniformis T5 was cloned into the pET-28c(+) vector. Recombinant α-amylase was obtained by plasmid expression in Escherichia coli strain ArcticExpressRP(DE3). Studies have shown that the pH and temperature optimum for the recombinant alpha-amylase was 6.5 and 80°C, respectively. As has been established that α-amylase is a calcium-independent enzyme. The study of the temperature stability of α-amylase showed that the enzyme retains more than 70% of its activity when α-amylase is incubated at 80°C for 30 minutes. The obtained results show a high prospect of using α-amylase from Bacillus licheniformis T5 as a thermostable enzyme in the technologies of enzymatic hydrolysis of starch-containing raw materials.
Analisis Kualitas Website Sistem Langitan Umaha Dengan Webqual 4.0 dan Importance Performance Analysis
Authors: Pratama Arista , Larasati Adelia Sefri , Wulansari Anita 
Published: March 2022

Perguruan tinggi sebagai suatu lembaga penyedia jasa pendidikan diwajibkan untuk memberikan pelayanan yang maksimal. Pemanfaatan teknologi informasi dapat dilakukan melalui penerapan sistem informasi akademik. Berdasarkan hal tersebut, Universitas Maarif Hasyim Latif (Umaha) membangun Sistem Langitan. Sistem Langitan merupakan sistem informasi akademik yang berbasis website yang dapat diakses kapan saja dan dimana saja asalkan tersambung dengan koneksi internet. Dengan adanya sistem informasi akademik mahasiswa dapat mengakses informasi yang berkaitan dengan catatan akademik selama proses perkuliahan. Tujuan penelitian ini untuk mengetahui kualitas website Sistem Langitan Umaha dengan menggunakan metode Webqual dan Importance Performance Analysis (IPA). Penelitian ini menggunakan Webqual sebagai dasar pembuatan kuesioner dan Importance Performance Analysis (IPA) sebagai alat analisa berupa Gap Analysis dan kuadran kartesius. Penelitian ini menggunakan proportionate stratified random sampling dengan jumlah responden sebanyak 340 responden yang merupakan mahasiswa aktif di Universitas Maarif Hasyim Latif.Berdasarkan hasil analisis diperoleh bahwa gap analysis keseluruhan bernilai 0,13 yang menujukkan hasil dari kinerja website Sistem Langitan sudah memenuhi kepentingan dan harapan pengguna. Namun masih ada beberapa indikator yang belum memenuhi kepentingan dan harapan pengguna. Hasil analisis Importance Performance Analysis (IPA) diperoleh bawah 7 indikator berada di kuadran pertama yang menjadi prioritas utama untuk diperbaiki karena kualitas website belum memenuhi kepentingan dan harapan pengguna. 8 indikator berada di kuadran kedua yang kualitasnya sudah sesuai dengan harapan dan kepentingan pengguna sehingga hanya perlu mempertahankan kualitas yang sudah ada. 5 indikator berada dikuadran ketiga yang dikembangkan sesuai dengan kepentingan dan harapan pengguna sehingga hanya perlu mempertahankan kualitas yang sudah ada. 7 indikator berada di kuardan keempat ini kinerjanya cenderung berlebihan sehingga disarankan untuk memfokuskan pengembangan atau perbaikan tingkat kinerja indikator di kuadran pertama.
Pengujian Multicore Pada Processor Terhadap Performansi Server Virtualisasi Menggunakan Metode Load Testing
Authors: Ferdiansyah Doddy , Kamal Aliev Riaunanda , Majapahit Sali Alas , Mulyanto Ferry 
Published: March 2022

Dalam membangun sebuah laboratorium keamanan informasi, perlu diperhatikan aspek perangkat, aplikasi, dan lingkungannya (environment). Laboratorium keamanan informasi ini bertujuan untuk menguji tingkat keamanan dari sebuah aplikasi yang akan atau sudah dibangun. Tetapi yang menjadi masalah utama adalah sulitnya pemilihan jenis perangkat keras yang sesuai dengan kebutuhan. Ada beberapa parameter yang harus diperhatikan dalam menentukan komponen perangkat keras yang tepat, yaitu Random Access Memeory (RAM), Processor, dan Network Interface Card (NIC).
Dalam penelitian ini, hanya berfokus pada pengujian pengaruh Multi Core dalam sebuah Processor. Seperti yang diketahui, Processor merupakan perangkat utama yang sangat penting dalam komputer. Jika diibaratkan, Processor merupakan otak dari komputer yang akan digunakan untuk menguji tingkat keamanan dari sebuah aplikasi yang akan atau sudah dibangun. Selain itu, pemilihan jenis Processor juga sangat berpengaruh dalam pemrosesan tugas yang akan dilakukan oleh komputer uji dalam rancangan pembangunan laboratorium kemanan informasi ini, sehingga pemilihan Processor sangatlah penting.
Hasil akhir dari penelitian ini adalah untuk mendapatkan rekomendasi Processor yang sesuai dengan kebutuhan komputer uji pada Blueprint laboratorium kemanan informasi
Stepping beyond perfectly mixed conditions in soil hydrological modelling using a Lagrangian approach
Authors: Sternagel Alexander , Loritz Ralf , Berkowitz Brian , Zehe Erwin 
Published: March 2022

Abstract. A recent experiment of Bowers et al. (2020) revealed that diffusive mixing of water isotopes (δ2H and δ18O) over a fully saturated soil sample of a few centimetres in length required several days to equilibrate completely. In this study, we present an approach to simulate such time-delayed diffusive mixing processes, on the pore scale, beyond instantaneously and perfectly mixed conditions. The diffusive pore mixing (DIPMI) approach is based on a Lagrangian perspective on water particles moving by diffusion over the pore space of a soil volume and carrying concentrations of solutes or isotopes. The idea of DIPMI is to account for the self-diffusion of water particles across a characteristic length scale of the pore space using pore-size-dependent diffusion coefficients. The model parameters can be derived from the soil-specific water retention curve, and no further calibration is needed. We test our DIPMI approach by simulating diffusive mixing of water isotopes over the pore space of a saturated soil volume using the experimental data of Bowers et al. (2020). Simulation results show the feasibility of the DIPMI approach for reproducing the measured mixing times and concentrations of isotopes at different tensions over the pore space. This result corroborates the finding that diffusive mixing in soils depends on the pore size distribution and the specific soil water retention properties. Additionally, we perform a virtual experiment with the DIPMI approach by simulating mixing and leaching processes of a solute in a vertical, saturated soil column and compare the results against simulations with the common perfect mixing assumption. The results of this virtual experiment reveal that the frequently observed steep rise and long tailing of breakthrough curves, which are typically associated with non-uniform transport in heterogeneous soils, may also occur in homogeneous media as a result of imperfect subscale mixing in a macroscopically homogeneous soil matrix.
Is it north or west foehn? A Lagrangian analysis of Penetration and Interruption of Alpine Foehn intensive observation period 1 (PIANO IOP 1)
Authors: Saigger Manuel , Gohm Alexander 
Published: March 2022

Abstract. A case study of a foehn event in the Inn Valley near Innsbruck, Austria, that occurred on 29 October 2017 in the framework of the first intensive observation period (IOP) of the Penetration and Interruption of Alpine Foehn (PIANO) field campaign is investigated.
Accompanied with northwesterly crest-level flow, foehn broke through at the valley floor as strong westerly winds in the morning and was terminated in the afternoon by a cold front arriving from the north.
The difference between local and large-scale wind direction raises the question of whether the event should be classified as north or west foehn – a question that has not been convincingly answered in the past for similar events based on Eulerian approaches. Hence, the goal of this study is to assess the air mass origin and the mechanisms of foehn penetration to the valley floor based on a Lagrangian perspective. For this purpose a mesoscale simulation with the Weather Research and Forecasting (WRF) model and a backward trajectory analysis with LAGRANTO are conducted. The trajectory analysis shows that the major part of the air
arriving in Innsbruck originates 6 h earlier over eastern France, crosses the two mountain ranges of the Vosges and the Black Forest, and finally impinges on the Alps near Lake Constance and the Rhine Valley.
Orographic precipitation over the mountains leads to a net diabatic heating of about 2.5 K and to a moisture loss of about 1 g kg−1 along the trajectories.
A secondary air stream originates further south over the Swiss Plateau and contributes about 10 % to 40 % of the trajectories to the foehn air in Innsbruck. Corresponding trajectories are initially nearly parallel to the northern Alpine rim and get lifted above crest level in the same region as the main trajectory branch.
Air parcels within this branch experience a net diabatic heating of about 2 K and, in contrast to the ones of the main branch, an overall moisture uptake due to evaporation of precipitation formed above these air parcels.
Penetration into the Inn Valley mainly occurs in the lee of three local mountain ranges – the Lechtal Alps, the Wetterstein Mountains, and the Mieming Chain – and is associated with a gravity wave and a persistent atmospheric rotor. A secondary penetration takes place in the western end of the Inn Valley via the Arlberg Pass and Silvretta Pass. Changes in the upstream flow conditions cause a shift in the contributions of the associated penetration branches.
From a Lagrangian perspective this shift can be interpreted on the valley scale as a gradual transition from west to northwest foehn despite the persistent local west wind in Innsbruck. However, a clear classification in one or the other categories remains subjective even with the Lagrangian approach and, given the complexity of the trajectory pattern, is nearly impossible with the traditional Eulerian view.
A simplified, efficient approach to hybrid wind and solar plant site optimization
Authors: Tripp Charles , Guittet Darice , King Jennifer , Barker Aaron 
Published: March 2022

Abstract. Wind plant layout optimization is a difficult, complex problem with a large number of variables and many local minima. Layout optimization only becomes more difficult with the addition of solar generation. In this paper, we propose a parameterized approach to wind and solar hybrid power plant layout optimization that greatly reduces problem dimensionality while guaranteeing that the generated layouts have a desirable regular structure. Thus far, hybrid power plant optimization research has focused on system sizing. We go beyond sizing and present a practical approach to optimizing the physical layout of a wind–solar hybrid power plant. We argue that the evolution strategy class of derivative-free optimization methods is well-suited to the parameterized hybrid layout problem, and we demonstrate how hard layout constraints (e.g., placement restrictions) can be transformed into soft constraints that are amenable to optimization using evolution strategies. Next, we present experimental results on four test sites, demonstrating the viability, reliability, and effectiveness of the parameterized evolution strategy approach for generating optimized hybrid plant layouts. Completing the tool kit for parameterized layout generation, we include a brief tutorial describing how the parameterized evolutionary approach can be inspected, understood, and debugged when applied to hybrid plant layouts.
Tridimensional roughness and morphology of sound dentin surfaces after papain-gel treatment
Authors: Pires Paula Maciel , Miranda Paula Martins Bravo , Costa Paula Helena de Accioly , Monteiro Amanda Souza Nunes , Alexandria Adílis Kalina , Cople Maia Lucianne , Neves Aline De Almeida 
Published: March 2022

Purpose: To investigate the effect of chemomechanical caries removing agents (CCRAs) based on papain (Papacárie Duo Gel® and Brix3000®) over dentin surfaces compared with 37% phosphoric acid and 11.5% polyacrylic acid.Materials and methods: Sound human molars were sectioned at the crown level, resulting in 48 dentin blocks, which were randomly divided into 4 groups (n=12): 1) Papacárie Duo Gel®; 2) Brix3000®; 3) 11.5% polyacrylic acid solution and 4) 37% phosphoric acid gel. All products were applied for 30s. Ten blocks per group were analyzed by a non-contact 3D profilometer before and after treatments for linear (Ra) and volumetric roughness (Sa). The superficial morphology of the remaining blocks in each group (n=2) was evaluated by scanning electron microscopy (SEM). Normality was rejected for the data (Shapiro-Wilk test) and therefore, Kruskal-Wallis test, followed by Dunn’s proof or Wilcoxon signed rank test with its respective effect size calculation were used to compare the results with α=5%.Results: Ra and Sa values for specimens submitted to Papacárie Duo Gel® and Brix3000® were statistically similar to baseline values. Application of phosphoric and polyacrylic acid resulted in a statistically increase in roughness compared to the CCRAs. SEM evaluation showed that Papacárie Duo Gel® resulted in surface debris. Polyacrylic acid and Brix3000®resulted in partial opening of the tubules but dentin exposed to polyacrylic was able to remove more smear layer than Brix3000®, while phosphoric acid resulted in total opening of the dentinal tubules.Conclusion: Both Papacárie Duo Gel® and Brix3000® did not result in roughness changes when applied in sound dentin.
Correction of postoperative cranialgia in neurosurgical patients
Authors: Masyutina D. D. , Goncharov M. Ju. 
Published: March 2022

Introduction.According to various data, postcraniotomy headache (PCH) is observed in 60-80% of cases, but so far this problem has not been given due attention. Non-modifiable risk factors affecting the intensity of PCG are female sex, young age, anxiety and depressive disorders; the main modifiable factor is craniotomy access.The aim of this studyis to improve the outcomes of neurosurgical interventions by reducing the severity of postoperative cephalalgia.Materials and methods.A comparative analysis of the severity of postcraniotomy headache in the early and immediate postoperative period was carried out in 2 groups of patients: with classical osteoplastic craniotomy (control group) and using a low-trauma technique for removing intracerebral hematoma (main group). To assess the level of cranial pain, a digital pain rating scale was used.Results.The average indicators of the intensity of cephalalgia in the early period in the control group was 7.5 ± 1.5, in the main group 6.1 ± 1.2, in the nearest: the control group 5.6 ± 1.4, the main one – 3.9 ± 1.1 (p<0.05).Discussion.The reduction in the volume of the surgical wound significantly led to a decrease in the intensity of the postoperative cephalgic syndrome. A number of techniques have been proposed in the literature as surgical and medical methods for the prevention of postcraniotomy headache, but they do not have an adequate evidence base and require further study. A decrease in the intensity of PCG helps to improve the quality of life of patients and to reduce the financial costs required for its treatment.Conclusions.The use of a minimally traumatic technique of intracerebral hematoma removal contributes to the reduction of postoperative cephalgia in patients in the immediate postoperative period.
Medical and social portrait and clinical features of patients with endometrial hyperplasia
Authors: Safronov O. V. , Kazachkova E. A. , Kazachkov E. L. , Safronova L. E. , Safronova I. V. 
Published: March 2022

Introduction. Today more and more attention is paid to the influence of various external factors and health status on the development of specific features of the course of different diseases, that is to the pathomorphosis in its clinical (narrow) sense.The aim of the study was comparative analysis of the medical and social profile and clinical features of the patients with endometrial hyperplasia (EH) without atypia in the cohorts of 2016-2018 and 2006-2008 in the light of the pathomorphosis study.Materials and methods. A retrospective study of medical and social profile and clinical features of EH without atypia has been performed in 52 patients who were under observation in 2016 – 2018 (1st group) and in 52 women who underwent medical treatment of this disease in 2006-2008 (2nd group) to investigate the problem of EH without atypia from the point of view of the pathomorphosis study.Results. The following are the characteristic features of today’s patients with EH without atypia: younger age of disease development, specific formation of menstrual function (a later menarche), specific somatic status (predominance of the diseases of the urinary system and gastrointestinal tract), predominance of non-inflammatory diseases of the reproductive organs over sexually transmitted infections, low parity, complicated course of labor (increase in the proportion of premature delivery). Heavy menstrual bleeding (HMB) was the main clinical manifestation of the EH without atypia.Discussion. Our data on the clinical picture of EH without atypia and the high frequency of the combination of EH with chronic endometritis coincide with the results of other modern studies. However, at present, in the clinical picture of EH, along with HMB, we observe a tendency to an increase in the frequency of asymptomatic EH, and the revealed high frequency of the combination of EH with chronic endometritis confirms the conclusions of a number of researchers about the important role of chronic endometritis in the formation of EH.Conclusions. The above stated specific features of the medical and social profile and clinical course of the EH without atypia could be interpreted as pathomorphosis in the clinical (narrow) sense.
Predicting relapse in demyelinating diseases in children
Authors: Nevmerzhitskaya K. S. , Volkova L. I. , Sergeeva M. V. 
Published: March 2022

Introduction. Predicting relapse in acute demyelinating episode (ADE) in children is an urgent problem, since progressive demyelinating diseases are associated with the risk of disability and cognitive impairment.Methods. Descriptive cohort study. The results of long-term follow-up of 75 children after the first episode of demyelination are presented. Based on the clinical and radiological parameters of the first demyelinating event, the prognostic factors for the relapse in children were determined using the logistic regression method.Results. When comparing the clinical and instrumental signs of the first demyelinating event, we identified those that were significantly associated with relapse. These included age ≥ 11 years (p <0.001), brain stem symptoms (p = 0.002), multiple demyelinating lesions on brain magnetic resonance imaging (p = 0.001) periventricular (p = 0.002), subcortical (p = 0.001), brainstem lesions (p = 0.006), well-defined lesions (p = 0.03) and perpendicular to the corpus callosum lesions (p = 0.002), cervical spinal cord lesions (p = 0, 02) and lateralized spinal cord lesions (p = 0.02). Regression analysis showed independent risk factors for relapse in children with demyelinating diseases: age ≥ 11 years (OR = 1.34, 95% CI (1.11: 1.61), p = 0.003), brain stem symptoms (OR = 7.00, 95% CI (0.73: 67.25, p = 0.09), multiple CNS lesions, corresponding to the criteria for dissemination by McDonald (2010) (OR = 8.60, 95% CI (2, 24: 33.07), p = 0.002).Discussion. Existing descriptions of pediatric populations with demyelinating diseases often have short follow-up and focus on outcomes in multiple sclerosis and neuromyelitis optica. The article presents data on previously unexplored risk factors for exacerbation after the first episode of demyelination. Conclusion. The identified predictors of relapse in ADE in children are a simple and generally available tool for predicting the course of demyelinating diseases.
Severity of depressive symptomatology and socio-demographic characteristics of patients with alcoholism in the Republic of Dagestan depending on territorial patterns of residence
Authors: Shamsieva S. R. , Mollaeva N. R. , Lebedeva Dz. I. 
Published: March 2022

Introduction. Living in urban and rural areas can affect the risk of developing and the course of alcohol dependence and comorbid conditions. The aim of the work was to evaluate the degree of depressive symptoms and socio-demographic characteristics of patients with alcoholism in the Republic of Dagestan, depending on the territorial features of residence. Materials and methods. The study involved 104 patients aged 51.2±9.9 years (27 to 74 years), registered in the State Budgetary Institution «Republican narcological dispensary» of the Republic of Dagestan with a diagnosis of alcohol dependence. The patients were divided into 2 groups depending on the territorial features of their residence: 62 (59.6%) patients residing in urban areas and 42 (40.4%) residing in rural areas. In order to evaluate socio-demographic characteristics all patients were questioned. The degree of severity of depressive symptoms was assessed with the Beck scale. Results. Analysis of alcohol dependence characteristics revealed no differences between the groups (p>0.05). The degree of depression was found to be more pronounced in patients living in rural areas than in those living in urban areas: mild depression was 7.3% and 29.0% (p<0.05), moderate depression was 9.8% and 1.6% (p<0.05), and severe depression was 39.0% and 25.8% (p<0.05), respectively. The severity of depression correlated with the presence of work in patients (r=0.324; p<0.001). Discussion. Alcohol consumption leads to a more severe manifestation of depression, contributing to physiological changes in the body. Conversely, depressed people are more prone to uncontrolled alcohol consumption, which in their subjective opinion alleviates psychosocial suffering. The connection between alcohol abuse and depressive symptoms is bidirectional, meaning that both of these disorders influence the development and course of the other and simultaneously exacerbate the risk of developing the other disorder. Conclusions. Alcohol-dependent individuals living in rural areas of the Republic of Dagestan are characterized by unemployment that correlates with anxiety-depressive symptoms, as well as a more severe degree of depression severity.