bluuebunny/crossref_metadata_2025_split huggingface dataset:
Obesity-mediated upregulation of the YAP/IL33 signaling axis promotes an immunosuppressive tumor microenvironment in breast cancer
Authors: Dai Jia-Zih , Yang Ching-Chieh , Shueng Pei-Wei , Wang Yen-Ju , Huang Chen-Shiuan , Chao Yi-Chun , Lin Cheng-Wei 
Published: September 2022

Abstract
Obesity is a well-known risk factor for breast cancer formation and is associated with elevated mortality and a poor prognosis. An obesity-mediated inflammatory microenvironment is conducive to the malignant progression of tumors. However, the detailed molecular mechanism is still needed to be clarified. Herein, we identified that breast cancer cells from mice with diet-induced obesity exhibited increased growth, invasiveness, and stemness capacities. A transcriptome analysis revealed that expressions of interleukin 33 (IL33) signaling pathway-related genes were elevated in obesity-associated breast cancer cells. Importantly, IL33 expression was significantly associated with the yes-associated protein (YAP) signature, and IL33 was transcriptionally regulated by YAP. Suppression of IL33 reduced tumor migration and invasion, while the addition of IL33 activated nuclear factor (NF)-κB signaling and revived tumor mobility in YAP-silenced cells. Furthermore, suppression of YAP attenuated IL33 expression which was accompanied by relief of obesity-mediated immunosuppression. Clinical analyses showed that IL33 expression was markedly associated with macrophage and regulatory T cell infiltration. These findings reveal a crucial role of the YAP/IL33 axis in promoting aggressiveness and immunosuppression of obesity-associated breast cancer progression.
Development and use of an ESBL coding gene panel in patients undergoing first-line eradication therapy for Helicobacter pylori
Authors: Gudra Dita , Silamikelis Ivars , Pjalkovskis Janis , Danenberga Ilva , Pupola Darta , Skenders Girts , Ustinova Maija , Megnis Kaspars , Leja Marcis , Vangravs Reinis , Fridmanis Davids 
Published: September 2022

Abstract

The spread of extended-spectrum beta-lactamases (ESBLs) in nosocomial and community-acquired enterobacteria is an important challenge for clinicians due to the limited therapeutic options for infections that are caused by these organisms. The epidemiology of these infections is complex and combines the expansion of mobile genetic elements with clonal spread. Insufficient empirical therapy for serious infections caused by these organisms is independently associated with increased mortality. Here, we developed an ESBL coding gene panel, evaluated the abundance and prevalence of ESBLs encoding genes in patients undergoing H. pylori eradication therapy, and summarized the effect of eradication therapy on gut microbiome functional profiles. To assess the repertoire of known beta lactamase (BL) genes, we divided them in clusters according to their evolutionary relation, designed primers for amplification of cluster marker regions and assessed efficiency of this amplification panel on 120 fecal samples acquired from 60 patients undergoing H. pylori eradication therapy. In addition, fecal samples from additional 30 patients were used to validate the detection efficiency of designed ESBL panel. The presence for majority of targeted clusters was confirmed by NGS of amplification products. Metagenomic sequencing revealed that the abundance of ESBL genes within the pool of microorganisms was very low. The global relative abundances of the ESBL-coding gene clusters did not differ significantly across the treatment states. However, at the level of each cluster, classical ESBL producers, such as Klebsiella sp. for blaOXY (p = 0.0076), Acinetobacter sp. for blaADC (p = 0.02297), and others, differed significantly with a tendency to decrease compared to the pre- and post-eradication states. Only 13 clusters were common among all three datasets, suggesting a patient-specific prevalence profile of ESBL-coding genes. The number of AMR genes detected in the post-eradication state was higher than that in the pre-eradication state, which at least partly might be attributed to the therapy. This study demonstrated that the ESBL screening panel was efficient for targeting ESBL-coding gene clusters from bacterial DNA and that minor differences exist in the abundance and prevalence of ESBL-coding gene levels before and after eradication therapy.
Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93
Authors: Zhao Hongbo , Karppi Johanna , Nguyen Thi Truc Minh , Bellemare Annie , Tsang Adrian , Master Emma , Tenkanen Maija 
Published: September 2022

Abstract
Background
The Carbohydrate-Active enZymes (CAZy) auxiliary activity family 3 (AA3) comprises flavin adenine dinucleotide-dependent (FAD) oxidoreductases from the glucose-methanol-choline (GMC) family, which play auxiliary roles in lignocellulose conversion. The AA3 subfamily 1 predominantly consists of cellobiose dehydrogenases (CDHs) that typically comprise a dehydrogenase domain, a cytochrome domain, and a carbohydrate-binding module from family 1 (CBM1).
Results
In this work, an AA3_1 gene from Thermothelomyces myriococcoides CBS 398.93 encoding only a GMC dehydrogenase domain was expressed in Aspergillus niger. Like previously characterized CDHs, this enzyme (TmXdhA) predominantly accepts linear saccharides with β-(1→4) linkage and targets the hydroxyl on the reducing anomeric carbon. TmXhdA was distinguished, however, by its preferential activity towards xylooligosaccharides over cellooligosaccharides. Amino acid sequence analysis showed that TmXdhA possesses a glutamine at the substrate-binding site rather than a threonine or serine that occupies this position in previously characterized CDHs, and structural models suggest the glutamine in TmXdhA could facilitate binding to pentose sugars.
Conclusions
The biochemical analysis of TmXdhA revealed a catalytic preference for xylooligosaccharide substrates. The modelled structure of TmXdhA provides a reference for the screening of oxidoreductases targeting xylooliogosaccharides. We anticipate TmXdhA to be a good candidate for the conversion of xylooligosaccharides to added-value chemicals by its exceptional catalytic ability.
The spatial and temporal evolution of mineral discoveries and their impact on mineral rarity
Authors: Ponomar Vitalii , Gavryliv Liubomyr , Putiš Marián 
Published: September 2022

Abstract
This paper presents the proceedings of the data analysis of the year and country of mineral discoveries with their Nickel-Strunz classes and rarity to enrich our knowledge of the evolution of mineral discoveries and their spatial distribution during different periods. Based on the dynamic of mineral discovery, three principal periods were identified: (1) Ancient period (up to 1800) of irregular mineral records; (2) Sustainable development period (1800–1949) with regular records and a moderate increase in the total number of minerals; and (3) Modern period (1950–present) of rapid development. It is pointed out that the timeline of mineral discoveries exhibits local anomalies. The positive anomalies were linked to the publications of mineralogical encyclopedias and classifications, while the negative ones were caused mainly by historical events, suppressing scientific activity. The majority of rock-forming and widespread minerals were discovered before the 1980s, while the discovery rate of rare and endemic species still progresses due to the study of hard-to-reach locations and the introduction of high-resolution analytical methods. A comparison of Nickel-Strunz class counts throughout mineral history revealed that the fraction of carbonates, oxides, and elements have drastically decreased during the Sustainable development period and the Modern period with a minor increase of elements during the last period. However, opposite behavior is observed for the phosphates, sulfates, and sulfides, with a sudden decrease in sulfates during the Modern period. On the other hand, the fraction of borates, halides, and silicates remained unchanged during all periods. Spatial analysis of the data showed that the distribution of mineral discoveries on the world map depends not only on the country’s geology but also on the area, population, economic development, and general interest in science.
The effectiveness of a plant integrated lowcost treatment system to treat well water in coastal regions
Authors: Rathnayaka R. M. C. Y. , Amarathunga A. A. D. , Bellanthudawa B. K. A. , Rathnasri P. A. S. A. , Novak A. T. , Amarasiri C. 
Published: September 2022

Abstract
The present study aimed to quantify the treatment capacities of medicinal plant materials integrated into low-cost water treatments system (LCWTS) to treat well water in the coastal regions of Jaffna and Dikowita in Sri Lanka. Terminalia arjuna roots, Strychnos potatorum seeds, and Phyllanthus emblica leaves were employed to design LCWTS. Paired t-tests were performed to explore water quality parameters of well water before and after treatment. Treated well water was compared with the Sri Lanka Standard Institute and Central Environment Authority. The water quality parameters of treated water samples were significantly different (p &lt; 0.05) in three filter media. Further, ammonia, total hardness, and alkalinity concentrations in drinking water exceeded the tolerance limits. The most effective LCWTS was Terminalia arjuna while Strychnos potatorum seeds was the least effective LCWTS. Terminalia arjuna roots were the most effective filter medium due to its specific phytoremediation capabilities for water filtration and Phyllanthus emblica leaves were good in adsorbing contaminants in water. Hence, the present study showed that Terminalia arjuna and Phyllanthus emblica can be used as potential LCWTS to treat the water quality of total hardness, conductivity, ammonia, total suspended solids, alkalinity, and chloride concentrations.
The Search for New Antitumor Drugs from Blue-Green Algae
Authors: Moore Richard E. , Corbett Thomas H. , Patterson Gregory M. L. , Valeriote Frederick A. 
Published: September 2022

Abstract:
Six to ten percent of the extracts of laboratory-cultured blue-green algae are significantly cytotoxic against human tumor cell lines. Structurally-unique cytotoxins have been isolated from most of the active extracts. Of the extracts of >2000 cyanophytes examined to date, approximately 0.8% showed solid tumor selective cytotoxicity in the Corbett assay. The lipophilic extract of Nostoc sp. GSV 224 was by
far the most cytotoxic and one of the few extracts that were both solid tumor and tumor selective. Cyclic depsipeptides called cryptophycins were found to be the solid tumor and tumor selective agents. In vivo studies on naturally-occurring cryptophycin-1 and semi-synthetic cryptophycin-8 indicate that these compounds are very active against a broad spectrrum of tumors implanted in mice, including multidrug-resistant tumors, and that a cryptophycin analog may eventually be evaluated in the clinic. Several cytotoxins from terrestrial cyanophytes have•structures that resemble those of some antineoplastic agents from marine invertebrates, suggesting that a more extensive cultivation and screening effort on marine cyanophytes could lead to more discoveries of new antitumor drugs.
Lumbar Solitary Osteochondroma with Lower Extremity Weakness: A Case Report
Authors: Sato Takahiro , Kinoshita Hayato , Kobayashi Takashi , Miyakoshi Naohisa 
Published: September 2022

Background:
Osteochondroma is the most common benign bone tumor. However, osteochondroma of the spine is relatively rare, accounting for only 4% to 7% of primary benign spinal tumors and less than 3% of all osteochondromas. Furthermore, symptomatic osteochondroma in the lumbar spine is much rarer. We herein describe a patient who was diagnosed with a lumbar solitary osteochondroma causing neurological symptoms of the lower extremities. The patient successfully underwent surgical removal of the lesion.

Case Presentation:
A 79-year-old man presented with a 3-year history of difficulty walking and gradual development of numbness in his left upper extremity, which further exacerbated his difficulty walking. Magnetic resonance imaging and computed tomography revealed spinal stenosis at C3/4, L4/5, and L5/S1 and an osseous solitary lesion. We performed a single-stage operation of both the cervical and lumbar spine. The osseous solitary lesion at L5/S1 was removed, and the pathological diagnosis was osteochondroma. After the operation, the patient’s upper extremity numbness and walking ability were improved.

Conclusions:
We experienced a rare case of a neurologically symptomatic solitary osteochondroma in the lumbar spinal canal. Moreover, this lesion was found at the same time as cervical spondylosis causing numbness. Therefore, we must perform careful examinations and thoroughly evaluate imaging and postoperative pathological findings.
AIMS65 and PALBI Scores as Predictors of Six Months’ Mortality in Cirrhotic Patients with Acute Variceal Bleeding
Authors: Elhendawy Mohammed , Eldesouky Alshaimaa Fathy , Soliman Shaimaa S , Mansour Loai , Abd-Elsalam Sherief , Hawash Nehad 
Published: September 2022

Background & Aims:Bleeding gastroesophageal varices are a cause of high mortality among cirrhotic patients. We aimed to investigate late mortality predictors and prognostic models using easily verified factors at admission in cirrhotic patients with acute variceal bleeding (AVB).Methods:Between January 2020 and June 2020, 142 patients with AVB from Tanta university hospital were included. Investigating multiple prognostic models was done using multiple logistic regression after identifying significant predictors of 6 months' mortality. Mortality prediction accuracy was assessed with area under the receiver operating characteristic (AUROC) curve.Results:The 6 months’ overall mortality rate was 31% (44 patients had died). AIMS56, Child-Turcotte-Pugh (CTP) grade C and MELD scores were significantly higher among non survivors (p<0.001) while Platelet-albumin-bilirubin (PALBI) was significantly more negative among survivors (P=0.001). Hepatocellular carcinoma was not significantly related to the mortality (p =0.364). Univariate analysis showed that high CTP, MELD, AIMS65 and PALBI scores were predictors of mortality and associated with decreased survival with high sensitivity and low specificity; while multivariate analysis showed that only AIMS56 was independently associated with mortality (p 0.004).Conclusion:CTP, MELD, AIMS65 and PALBI scores are simple, bed side risk scores that can be used for the prediction of 6 months’ mortality after AVB in cirrhotic patients with high sensitivities and lower specificities.
FORMULATION AND EVALUATION OF HERBAL SOAP TAKING DIFFERENT BIOACTIVE PLANTS BY COLD SAPONIFICATION METHOD
Authors: SHARMA SAFAL , PRADHAN SUSHILTA , PANDIT BIBHAS , MOHANTY JYOCHHANA PRIYA 
Published: September 2022

Objective: The final goal of this research is to develop and evaluate herbal bath soap taking various bioactive herbal plants extract with different ethinic and dermatological importance in ayurveda, namely Solanum lycopersicum, Sapindus mukorossi, Acacia nilotica, Citrus limon, Aloe barbadensis, Piper betle, Curcuma longa and Cocus nucifera.
Methods: The extract of all plant materials were obtained through various extraction procedures suitable for them. The extract was then mixed with lye and fatty oil for preparing a soap using the cold saponification method.
Results: The prepared soap was evaluated against marketed soap. The prepared soap was found to be good in appearance, color and odor. pH, % free alkali content, foamability, foam stability, moisture content and alcohol insoluble matter were found to be 9.6, 0.22, 15 cm, 14.5 cm, 3.26 and 17.25, respectively. The antioxidant activity and antibacterial studies were done, which signifies prepared soap to be a potent antioxidant and antibacterial source.
Conclusion: Based on the study results it can be concluded that herbal soap can be formulated using cold process method, taking different parameters in consideration as that of skin condition and as that of herbal potentials and its activity. This sought of herbal formulation can bring a big difference in the field of herbal cosmetic as there are many alignment and related flaws in different polyherbal or chemical-based formulations which can be removed.
Karakteristik Biopelet Dari Bahan Sekam Padi Dan Serbuk Gergaji Kayu Jati (Tectona grandis Linn.F)
Authors: Prasetyo Dhimas Mardyanto , Wulandari Febriana Tri , Webliana B Kornelia 
Published: September 2022

Pemanfaatan limbah sekam padi dan serbuk gergaji kayu jati sebagai biopelet menjadi salah satu solusi untuk menghindari masalah baru bagi lingkungan Tujuan dari penelitian ini adalah untuk mengetahui nilai sifat fisis biopelet dari bahan sekam padi dan serbuk gergaji kayu jati serta untuk mengetahui kualitas biopelet dari bahan sekam padi dan serbuk gergaji kayu jati. Penelitian ini dilakukan menggunakan metode eksperimen yang dibuat dengan rancangan acak lengkap non factorial. Pembuatan contoh uji dibuat berdasarkan 3 perlakuan yaitu sekam padi komposisi 100%, serbuk gergaji kayu jati komposisi 100%, dan campuran antara sekam padi dan serbuk gergaji kayu komposisi 50% : 50%. Pengujian dilakukan mengikuti Standar Nasional Indonesia (SNI) 8675 :2018. Hasil penelitian dianalisis menggunakan software spss 24, kemudian dilakukan uji lanjut menggunakan uji LSD dengan taraf kepercayaan 5%. Hasil penelitian menunjukkan bahwa formulasi terbaik dihasilkan oleh biopelet terdapat pada perlakuan serbuk gergaji kayu jati komposisi 100% dengan kadar air 3,03%; kadar abu 2,76%; kadar zat terbang 91,93%; kadar karbon terikat 4,24% dan nilai kalor 17,28 MJ/kg. Apabila dibandingkan dengan SNI 8675:2018, hasil perlakuan serbuk gergaji kayu komposisi 100% dari lima pengujian terdapat dua pengujian yang tidak lulus SNI yaitu kadar zat dan kadar karbon terikat. Sedangkan untuk perlakuan sekam padi komposisi 100% dan campuran komposisi 50% : 50% dari lima pengujian terdapat tiga pengujian tidak lulus SNI yaitu kadar abu, nilai kalor dan kadar karbon terikat. Hal ini diakibatkan karena adanya kandungan bahan baku berupa lignin, selulosa, sukrosa dan hemiselulosa yang berpengaruh terhadap tinggi rendahnya kadar abu, kadar zat terbang dan nilai kalor serta karbon terikat. Kemudian hasil uji lanjut terdapat interaksi antara sekam padi, serbuk gergaji kayu jati dan campuran sehingga berpengaruh nyata terhadap kadar air, kadar abu, kadar zat terbang, nilai kalor dan karbon terikat.
Vaccination and Mortality of Patients with a Novel Coronavirus Infection (COVID-19): A Global Approach
Authors: Benuni N. A. , Kotusov A. S. , Adzhieva F. S. , Kucher A. E. , Tolmacheva K. A. , Dushuk V. N. , Maslennikov R. V. 
Published: September 2022

Aim. The aim is to study the correlation between the vaccination rate (VR) and mortality rate of patients with COVID-19 (MpCOV).Methods. The countries with gross domestic product per capita corrected for purchasing power parity (GDP PPP) over $-10,000 were selected for an ecologic study. The city-states and countries with a population of 1,000,000 were excluded. The number of patients who died from COVID-19 within a week was divided by the number of patients diagnosed with COVID-19 within a week 20 days earlier to calculate MpCOV.Results. We included 85 countries. VR (r = –0.604; p < 0.001) and GDPpcPPP level (p = 0.202), is an independent determinant of MpCOV. There was no significant difference in MpCOV between groups of countries with VR < 20 % and 20–39 % (1.96 [1.21; 4.67] vs. 1.96 [1.01; 3.36] %; p = 0.464). MpCOV was higher in countries where VR were lower when groups of countries with VR of 20–39 %, 40–59 %, 60–79 %, and ≥80 % were compared (1.96 [1.01; 3.36] vs. 1.11 [0.76; 1.64] vs. 0.50 [0.39; 1.00] vs. 0.16 [0.10; 0.21]; p = 0.003; p = 0.020, and p = 0.008).Conclusions. An increase in VR correlates with a decrease in MpCOV.
Functional Disorders of the Gastrointestinal Tract During the Pandemic of a New Coronavirus Infection
Authors: Sheptulin A. A. , Piatenko E. A. 
Published: September 2022

Aim. To analyze the literature data devoted to the study of the features of functional gastrointestinal diseases (FGID) during the pandemic of a new coronavirus infection.Key findings. Measures taken in connection with the pandemic of COVID-19 infection (introduction of lockdown, social isolation) leads to an increase in the level of depression and anxiety and, as a consequence, to an increase in the frequency and prevalence of functional dyspepsia (FD) and irritable bowel syndrome (IBS), as well as an increase in the severity of clinical symptoms of these diseases. In turn, the overcoming of COVID-19 infection contributes to an increase in the permeability of the mucous membrane of the gastrointestinal tract and the occurrence of its inflammatory changes, which lead to the development of postinfectious (postcovid) FD and postinfectious (postcovid) IBS.Conclusion. The problem of functional gastrointestinal diseases during the pandemic of a new coronavirus infection is very important and requires further research.
Practical Recommendation of the Scientific Сommunity for Human Microbiome Research (CHMR) and the Russian Gastroenterological Association (RGA) on Small Intestinal Bacterial Overgrowth in Adults
Authors: Ivashkin V. T. , Maev I. V. , Abdulganieva D. I. , Alekseeva O. P. , Alekseenko S. A. , Zolnikova O. Yu. , Korochanskaya N. V. , Medvedev O. S. , Poluektova E. A. , Simanenkov V. I. , Trukhmanov A. S. , Khlynov I. B. , Tsukanov V. V. , Shifrin O. S. , Ivashkin K. V. , Lapina T. L. , Maslennikov R. V. , Fadeeva M. V. , Ulyanin A. I. 
Published: September 2022

Aim. To optimize the choice of treatment strategies by physicians and gastroenterologists to improve treatment and prevention of small intestinal bacterial overgrowth (SIBO) in adults.Key points. SIBO is a condition characterized by an increased amount and/or abnormal composition of the microbiota in the small intestine. Clinically, the syndrome is manifested by nonspecific gastroenterological complaints and the development of malabsorption syndrome. Most often, SIBO is associated with various chronic non- infectious diseases (both diseases of the gastrointestinal tract, and the cardiovascular system and the neuromuscular apparatus) and can affect the severity of their symptoms. Specific methods for diagnosing SIBO are the culture method and breath tests. The main approaches to the treatment of SIBO include the elimination of the underlying cause of its occurrence, the use of antibacterial drugs and adherence to dietary recommendations (elemental diet).Conclusion. Small intestinal bacterial overgrowth is common in patients with various diseases, but has non-specific manifestations, so proper diagnosis of this condition is required. SIBO therapy involves prescription of antibacterial agents, the most studied of which is the non-absorbable antibiotic rifaximin-α.
Analisis Kadar Rada'ah yang Mengharamkan Pernikahan Dalam Perspektif Hukum Islam
Authors: Zainal Abidin Ali 
Published: September 2022

Salah satu makanan terbaik yang dianugrahkan oleh Allah SWT adalah asi buat seorang bayi melalui perantara seorang ibu agar dapat terjamin kelangsungan hidupnya dikemudian hari, dan perempuan yang menyusukan tersebut menjadi ibunya dan diharamkan pernikahan denganya dan segenap keturunannya, itu konsekuensi atau efek hukum yang diatur oleh syariat. Perdebatan para ulama mazhab jumhur dan ibn hazm dan mazhab syafii dan mazhab imam ahmad bin hanbal, pasalnya apakah hubungan susuan terjadi seketika ketika bayi minum susu wanita? Jawabannya tidak karena harus melihat kadar susuan yang diminum bayi tersebut dan berapakah umur bayi yang menyusui? beberapa dalil dari al-Quran dan Hadis yang masih menjadi perdebatan di kalangan ulama dalam membahas masalah ini.