bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic analysis of novel multi-link-spring mechanism for vibration isolation and energy harvesting
Authors: Xie Jiaheng , Yang Tao , Tang Jie 
Published: December 2022

Abstract
The study of the effective combination of vibration isolation and energy harvesting (VIEH) has become a popular topic at present. However, due to technical limitation, existed vibration isolator integrated energy harvesters have poor performance at low frequency. Therefore, this paper proposes a new multi-link-spring mechanism (MLSM), which can achieve quiz-zero-stiffness (QZS) and multi-stable characteristic by adjusting the parameters. Subsequently, the VIEH performance of MLSM under harmonic excitation and Gaussian white noise excitation are studied. And then, the influence of different parameters on the VIEH performance of the MLSM is analyzed. It was found that the MLSM can achieve any small stiffness in the equilibrium position and has well vibration isolation performance under low frequency or even ultra-low frequency. The frequency band can be widened by adjusting parameters to achieve higher energy harvesting power. Finally, the MLSM is compared with two special cases. The results show that the new MLSM is basically the same as the other two oscillators in terms of vibration isolation but has better energy harvesting performance under the multi-stable characteristic. The MLSM provided by this paper is expected to reduce the impact of external excitation on high-precision sensitive equipment in special sites such as subway and mine, and at the same time supply power to structural health monitoring device.
Cannabidiol and Trazodone Ameliorate Neuronal Proteotoxicity in C. elegans
Authors: Gilberto Vincenzo S. , McGrath Stephanie , Alsulami Abdullatif M , Vidrio-Aguirre Alexia , Moreno Julie 
Published: December 2022

Abstract

Alzheimer’s disease (AD), typically characterized by the accumulation of the misfolded proteins Amyloid beta (Aβ1-42) and hyperphosphorylation of Tau (P-Tau), is the most common neurodegenerative disease. Although there is currently no cure, research with rodent models has found targeting the signaling pathways involved in reactive oxygen species (ROS) or the unfolded protein response (UPR) can diminish the toxic effects of AD (e.g., behavioral deficits, glial inflammation, and proteotoxicity). For this study, we hypothesized that treatment combining Cannabidiol (CBD) and Trazodone (TRA), would better mitigate neuronal dysfunction and extend the lifespan of nematodes engineered to aggregate toxic misfolded proteins. CBD and TRA have been individually found to diminish the deleterious effects of proteotoxicity in both in vivo and in vitro studies. The organisms utilized in the study included two strains of C. elegans genetically modified to express two pathological proteins of AD. Our experiments revealed that the motility and lifespan of C. elegans with proteotoxicity of either Aβ1-42 or P-tau were significantly improved by treatment with CBD and TRA in combination. We also confirmed aspects of existing research on the efficacy of CBD and TRA individually. Our data suggest that these benefits are seen with full-life, middle-age, and late-stage rescue utilizing CBD and TRA. These findings suggest the need for future experimentation incorporating CBD and TRA in treatment regiments in higher organisms, including neurodegenerative rodent models and aging canines with cognitive decline.
Sublingual Recurring Extra osseous Ewing Sarcoma: A Rare Case Report and Review of Literature
Authors: Amer Hatem , Alaroomy Leena , Ayad Haytham , El-din Yasmine Alaa , Alwadeai Mohamed 
Published: December 2022

Abstract
Background: Extera skeletal (Extraosseous) Ewing sarcoma is a rare primary tumor of the soft tissue, with an extremely high mortality rate and a bad prognosis which primarily affects young individuals. It occurs without particular clinical signs and can impact several different areas, which may delay diagnosis. Histopathological examination and immunohistochemistry are crucial for making a definite diagnosis. Besides this, early diagnosis is necessary for succeeding of the management.
Case report: The objective of the present case report was to document a rare case of EES which originated from the floor of the mouth. A 37-year-old male patient came to our clinic with sublingual rapidly growing swelling. He was suffering from difficulty in opening his mouth and a feeling of dysphagia. The patient revealed during a history-taking procedure that he had undergone one surgery to remove the mass that had previously developed in the same area, knowing that he had been diagnosed in the first as a non-specific inflammatory reaction. Clinical examinations revealed a dome shape soft tissue mass. The biopsy specimen underwent histopathological and immunohistochemical analysis, which demonstrated poorly differentiated small blue round cells with positive immuno-expression of CD-99, NKX2-2, FLI-1, and Vimentin. The final diagnosis was EES based on the clinical presentation, histopathological pattern, and findings of the immunohistochemistry investigations. Radiotherapy and chemotherapy were chosen as treatment modalities for the case. Unfortunately, the patient died after three months due to the occurrence of multiple distant metastasis areas.
Conclusion: Regardless of the relative rarity of Extraosseous Ewing sarcoma, it should be taken into account as a possible differential diagnosis for intraoral soft tissue masses, and accurate diagnosis with precise histopathological and immunohistochemical evaluations should be done as soon as possible. Moreover, improvement of management procedures of such cases generally provides the best chances for patient survival.
Comparison and Evaluation of the Effectiveness of Traditional Neuroanatomy Teaching in Medical Education with Virtual-Reality Application Based On 3D Virtual Neuroanatomical Models, which is an innovative approach
Authors: ALIM Ece , COŞKUN Özlem , PEKER Tuncay Veysel 
Published: December 2022

Abstract
Purpose:Learning the neuroanatomical structures is difficult in traditional medical education. Knowledge and visual materials in neuroanatomy books or atlases are static and limited with two dimensions. The limitation of cadaver and plastic models has been solved by the development of three-dimensional (3D) anatomical models using digital visualization technologies. Medical students are better able to understand the spatial topography of a large number of neuroanatomical structures that are condensed into a small region when they make use of 3D visualization technologies such as virtual reality (VR) and augmented reality (AR). Our study will open a new window to classical neuroanatomy education. We aimed to evaluate how much 3D neuroanatomical models based on VR application affect the success and motivation of medical school students in neuroanatomy courses.
Methods:Four exams were given to the second-year medical faculty students before the classical theoretical course (1), after the theoretical course (2), after the VR training and application (3), and six months later to evaluate the long-term effects of the training (4).
Results:Success averages were evaluated out of 10; It was found 3.38 in the students who participated in the evaluation after the traditional theoretical training, and 4.55 in the group who received VR training. In the long-term evaluation after six months, this average was found to be higher in the group that received VR training.
Conclusion:Our study fills an important gap in the literature in terms of demonstrating the positive effects of VR-based neuroanatomy training on memory in the long term.
Effect of heat treatments on microstructure and mechanical properties of AlSi10Mg alloys fabricated by Laser powder bed fusion
Authors: Zhu Xiaogang , Du Dafan , Dong Anping , Sun Qinyao , Sun Jing , Guo Lijie , Sun Baode , Chen Zhendong 
Published: December 2022

Abstract

This study investigated the microstructures and tensile properties of as-build and heat-treated (including T6, T2 and T6-T2 treatments) AlSi10Mg samples fabricated by laser powder bed fusion (LPBF). The microstructure analysis revealed that the trend of eutectic Al-Si phase distribution along grain boundaries for heat-treated samples was significantly weakened, and the eutectic Al-Si phase was transformed into Si particles which were uniformly distributed in α-Al matrix. For the T6-T2 treated specimens, the Si content in particles was the highest. Meanwhile, the T6 treatment increased the proportion of equiaxed grains on vertical section most significantly. However, in the wake of heat treatments, the average grain size on horizontal section had little change while the average grain size on vertical section was increased. The properties analysis demonstrated that the T6-T2 treated specimens obtained the best elongation and worst strength. Besides, the T6 treatment eliminated the anisotropy of the LPBFed specimens basically, and synergistically obtained the best combination of strength and plasticity. The T6 treatment weakened the direction difference to the greatest extent, whereas the T2 treatment minimized the stress concentration. According to the comparative finding, the Si content in particles played an important part in properties. Besides, the relationship among inverse pole figure (IPF) maps, pole figure (PF) maps, the mass fraction and distribution of Si in Al matrix and Si particle, the grain size, Kernel average misorientation (KAM) and mechanical properties under different heat treatments was established, which provided a new idea for the selection of heat treatments on LPBFed parts.
In vitro and in vivo antifungal activity of chitosan, as continuous gel and nanoparticles suspension, against Alternaria alternata and Penicillium expansum.
Authors:   Taís Téo de Barros-Alexandrino1, Martelli Milena Tosi , Assis Odílio Benedito Garrido 
Published: December 2022

Abstract

Formulations based on chitosan of medium molar mass, in the form of continuous gel and suspension of nanoparticles with average dimensions of 117 ± 20 nm, obtained by crosslinking with sodium tripolyphosphate, were evaluated with respect to the antimicrobial activity against the fungi Alternaria alternata and Penicillium expansum. When essayed in in vitro condition, using the sequential dilution method, the resultant minimum inhibitory concentration (MIC) was 7 gL− 1 for both fungi. In diffusion measurements on solid agar medium, the MIC of 7 gL− 1 was confirmed for the inhibition of A. alternata and 8 gL− 1 was found for P. expansum. Based on these results, the concentration of 9 gL− 1 was chosen for the in vivo tests in the form of coatings on previously inoculated apples and guavas. The coatings were prepared via nebulization, either from the continuous or the nanoparticulate formulation. Fungal counting (CFU) from inoculation day to fruit decay indicated a greater efficiency of the nanoparticulate coating, especially for guava, which is more perishable and susceptible to infestation. By comparison, on apples the coatings have similar initial inhibition. After 20 days of measurements however, the particulate system shows 13% higher efficiency than the continuous coating. For guava, the efficiency of the particulate suspension was even better, 33% higher than the continuous covering, having a significant effect on the extension of the shelf life, with an increase of one week under the experimental storage conditions evaluated (room temperature, RH 50–60%).
Phase 1 study to determine the safety and dosing of autologous PBMCs modified to present HPV16 antigens (SQZ- PBMC-HPV) in HLA-A*02+ patients with HPV16+ Solid Tumors
Authors: Jimeno Antonio , Baranda Joaquina , Iams Wade T. , Park Jong Chul , Mita Monica , Gordon Michael S. , Taylor Matthew , Dhani Neesha , Leal Alexis D. , Neupane Prakash , Eng Cathy , Yeku Oladapo , Mita Alain , Moser Justin C. , Butler Marcus , Loughhead Scott M. , Jennings Julia , Miselis Nathan R. , Ji Rui-Ru , Nair Nitya , Kornacker Martin , Zwirtes Ricardo F. , Bernstein Howard , Sharei Armon 
Published: December 2022

Abstract
Purpose
We conducted a dose escalation Phase 1 study of autologous PBMCs loaded by microfluidic squeezing (Cell Squeeze® technology) with HPV16 E6 and E7 antigens (SQZ-PBMC-HPV), in HLA-A*02 + patients with advanced/metastatic HPV16 + cancers. Preclinical studies in murine models had shown such cells resulted in stimulation and proliferation of antigen specific CD8 + cells, and demonstrated antitumor activity.
Methods
Administration of SQZ-PBMC-HPV was every 3 weeks. Enrollment followed a modified 3 + 3 design with primary objectives to define safety, tolerability, and the recommended Phase 2 dose. Secondary and exploratory objectives were antitumor activity, manufacturing feasibility, and pharmacodynamic evaluation of immune responses.
Results
Eighteen patients were enrolled at doses ranging from 0.5 × 106 to 5.0 × 106 live cells/kg. Manufacture proved feasible and required < 24 h within the overall vein-to-vein time of 1–2 weeks; at the highest dose, a median of 4 doses were administered. No DLTs were observed. Most related TEAEs were Grade 1–2, and one Grade 2 cytokine release syndrome SAE was reported. Tumor biopsies in three patients showed 2 to 8-fold increases in CD8 + tissue infiltrating lymphocytes, including a case that exhibited increased MHC-I + and PD-L1 + cell densities and reduced numbers of HPV + cells. Clinical benefit was documented for the latter case.
Conclusions
SQZ-PBMC-HPV was well tolerated; 5.0 × 106 live cells/kg with double priming was chosen as the recommended Phase 2 dose. Multiple participants exhibited pharmacodynamic changes consistent with immune responses supporting the proposed mechanism of action for SQZ-PBMC-HPV, including patients previously refractory to checkpoint inhibitors.
Active whey protein film containing milk-derived antimicrobial peptide for microbial control in soft cheese
Authors: Zhang Ruyue , Yang Yaling , Wang Bini , Zhang Fuxin , Liu Yufang 
Published: December 2022

Abstract
The aim of this study was to prepare whey protein-based edible films with the incorporation of milk-derived antimicrobial peptides (αs2-casein151 − 181 and αs2-casein182 − 207) and compare their effectiveness on the control of microbiological contamination in soft cheese with edible films containing nisin. These two antimicrobial peptides showed similar activity against B. subtilis and much higher activity against E. coli than bacteriocin nisin, representing that these milk-derived peptides had great potential to be applied as food preservative in dairy products. Antimicrobial peptides in whey protein films caused an increase of film thickness, opaqueness and water vapor barrier properties, but decreased the mechanical properties. Whey protein film incorporated with αs2-casein151 − 181 could inhibit the growth of yeasts and moulds, and control the growth of psychrotrophic bacteria present originally in the soft cheese at refrigerated temperature. It also exhibited the significantly inhibitory activity against the development of a mixed culture (E. coli and B. subtilis) in the cheese due to a superficial contamination during the storage. Antimicrobial peptides immobilized in whey protein films showed a higher effectiveness than its direct application in solution. In addition, films containing αs2-casein151 − 181 could act as a hurdle inhibiting the development of postprocessing contamination on the cheese surface during the 28 days of storage. Development of antimicrobial packaging on the basis of whey protein could be one effective way to reduce environment unfriendly plastic and whey protein waste for further application.
Transcriptomic Insight Based on Network Analysis Reveals the Effect of Ursolic Acid on Non-Alcoholic Steatohepatitis
Authors: Xue Chun-yan , Wu Ling , Rao Chen-yi , Huang Xiao-quan , Chen Shi-yao , Li Feng 
Published: December 2022

Abstract
Background
Non-alcoholic steatohepatitis (NASH) has attracted international attention. However, pharmaceutical treatments are not included in the current guidelines. Ursolic acid (UA) has the potential to treat metabolic disorders. Hence, this study aimed to investigate the impact of UA on NASH.
Methods
RNA sequencing from our own model mice was performed to detect differentially expressed genes (DEGs) in 12 mouse samples from 4 groups. DEGs were subjected to Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Weighted gene co-expression network analysis (WGCNA) was applied to find modules associated with NASH's pathological characteristics and identified the hub genes in the relevant modules. Hub genes were further identified and the ceRNA network was constructed. In vitro and in vivo experiments were applied to identify the expected mRNAs.
Results
By comparing NASH mice to Wild type (WT) mice, we performed GO and KEGG enrichment analysis to identify pathways associated with NASH inflammatory metabolic disorders, and by doing the same for NASH mice treated with UA, we identified pathways via which UA may alter. Based on WGCNA, the modules associated with the pathological changes of NASH, as well as the hub genes in each module were identified. A ceRNA network was successfully constructed. RT-PCR results showed that Akr1a1 and Chchd2 were protective factors against NASH, and Ndufb9 was a risk factor. The level of ROS detected by flow cytometry indicated that UA could alleviate oxidative stress.
Conclusion
Akr1a1, Chchd2, and Ndufb9 were essential for the therapeutic effects of UA on NASH and the ceRNA network might act as prospective therapeutic and diagnostic biomarker targets.
Diosmin nanocrystals alleviate Imiquimod induced psoriasis in rats via modulating TLR7,8/ NF-κB/micro RNA-31, AKT/mTOR/P70S6K milieu and Tregs / Th17 balance
Authors: Shahine Yasmine , El-Aal Sarah A. Abd , Reda Ahmed M. , Sheta Eman , Attia Nouran M. , Abdallah Ossama Y. , ibrahim Sherihan salaheldin abdelhamid 
Published: December 2022

Abstract
Diosmin is a flavonoidal compound characterized by highly challenging physicochemical properties. There wasn’t enough attention paid for using diosmin topically in spite of its strong anti-inflammatory and anti-oxidant properties. The aim of this work is the development and characterization of diosmin nanocrystals using anti-solvent precipitation technique to be used for topical treatment of psoriasis. Evaluation of different stabilizers with different concentrations to achieve the most stable nanocrystals was studied. Results revealed that diosmin nanocrystals stabilized with hydroxypropyl methylcellulose (HPMC E15) in weight ratio (diosmin:polymer 1:1) could reach the desired particle size (276.9 ± 16.49 nm); provided the promising colloidal properties and higher drug release profile. In-vivo assessment was carried out to evaluate and compare the activities of diosmin nanocrystals gel using 3 different doses and diosmin powder gel in alleviating imiquimod induced psoriasis in rats and investigating their possible anti-inflammatory mechanisms. Herein, 125 mg of 5% imiquimod cream (IMQ) was applied topically for 5 consecutive days on the shaved backs of rats to induce psoriasis. Diosmin nanocrystals gel especially in the highest dose used offered the best anti-inflammatory effect. This was confirmed by causing the most significant mitigation in the psoriasis area severity index (PASI) score and the serum inflammatory cytokines levels (IL17A, IL23, and IL22). Furthermore, it was capable of maintaining balance between Th17 and Treg cells by decreasing the immunohistochemical expression of RORγ and increasing that of FOXP3. Moreover, it tackled TLR7/8/NF-κB, AKT/mTOR/P70S6K and elevated the TNFAIP3/A20 (negative regulator of NF-κB) expression in psoriatic skin tissues. Also, it abrogated the tissue expression of PCNA, BCL-2 and miRNA-31 level. This highlights the role of diosmin nanocrystals gel in tackling imiquimod induced psoriasis in rats via modulating TLR7,8/NF-κB/miRNA-31, AKT/mTOR/P70S6K milieu and Tregs/Th17 balance. Therefore, it is suggested that diosmin nanocrystals gel could be a novel promising therapy for psoriasis.
Changes in the exposure to power saw noise during individual partial activities of tree felling in dependence on the stem wood moisture content
Authors: Staněk Luboš , Mergl Václav , Neruda Jindřich 
Published: December 2022

Abstract

This study deals with the level of noise to which power saw operators are exposed during partial activities of tree felling and persons moving in its surroundings. It also aims to reveal the existence of dependence between the level of produced noise and the moisture content of the felled tree by comparing the noise levels of individual partial activities. The process of tree felling was broken down into partial activities (pruning of the lower tree part; cutting off buttresses; felling; delimbing) during which exposure to noise caused by the power saw was measured. The noise was measured by the noise level meter at the operator’s ear at distances of 5 m and 10 m from the source of noise (power saw) in compliance with EN ISO 22868 and EN ISO 9612 standards. The noise was measured in a total of 121 trees processed by one power saw operator using one and the same type of power saw and one and the same type of saw chain. Research results revealed a noise level difference of individual operations in 88.10% of compared cases. Mean noise levels close to the operator’s ear ranged from 95,193.499 dB to 101,098.174 dB and mean noise values at a distance of 10 m ranged from 70,573.677 dB to 78,370.818 dB. A maximum noise level (115,720.000 dB) was measured during the partial operation of Delimbing near the operator’s ear; a minimum noise level (51,650000 dB) was recorded during this operation, too.
Modeling the impact of a family structure on household water consumption
Authors: Wang Mei , Fu Hanliang , Zhou Xinfa , Cheng Zhe 
Published: December 2022

Abstract
Household water consumption plays an important role in addressing the problem of water shortage and achieving sustainable water development. To identify, assess, and analyze the impact of a family structure on household water consumption, this study develops a mathematical statistical method to conduct multi-scenario simulations of average annual household water consumption based on data from the 2016 China Family Panel Studies (CFPS). The Kolmogorov–Smirnov test and the two independent sample t-tests were used to obtain the distribution with the highest degree of fitting, and the probability distribution and expected value of average annual household water consumption were obtained from the distribution probability function. The results demonstrated that the Birnbaum–Saunders distribution was the optimal distribution; families comprising one and two generations were dominant in terms of water consumption; and the number of water-saving households was far less than that of households with high levels of water consumption. The findings of this study have valuable implications for water governance and the domestic water planning.