bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of microRNA expression and target prediction in children with Nephrotic syndrome
Authors: Charmine Pricilla , Venkatesan Vettriselvi , Geminiganesan Sangeetha , Nammalwar Bollam Rangaswamy , Ekambaram Sudha , Priya C.D.Mohana 
Published: November 2022

Abstract
Background
Nephrotic syndrome is one of the common cause among the kidney disease in children worldwide .It is characterised by the edema proteinuria, hypoalbuminemia and hypocholestromia. Recently many studies have emerged with the association of microRNAs playing an potential roles in many pathophysiological functions. MicroRNAs(miRNAs ) and RNA binding proteins (RBPs) are found to be two most important needed transcriptional regulators of gene expression as well as for the aberrant expression that tend to contribute to the development of the disease. It can reduce translation neither by translation repression through or MicroRNA cleavage. In this present study we have checked for the expression pattern of the targeted microRNAs miR-17-5P,miR-155p ,miR- 424 -5p ,miR-1 and 215-5p in the Children among Steroid Sensitive Nephrotic syndrome(SSNS) Steroid Resistance Nephrotic Syndrome (SRNS)along with the healthy individuals. Total RNA was isolated from the urine samples among the three groups(SSNS = 100,SRNS = 100 and healthy individuals100).The expression pattern for theses microRNAs was carried out using RT-PCR .Bioinformatics tools such as miRWalk, miR-Tar link were used in predicting targets for the microRNAs an online data bases and g profiler software is used which was also helpful in evaluating the targets based on the biological functions, Molecular functions and the pathways related to the selected microRNAs, along with that ROC analysis was also performed which was widely helpful in selecting the microRNAs that could be used as a potential biomarker as well as a therapeutic target. Among the five microRNAs miR- 1,miR-215, miR-17-5P, ,miR-155-5p & miR-424-5p.Two microRNAs miR-424-5p& miR-155p is found to be up regulated in the SRNS group.
Loss of CD99L2 contributed to temozolomide resistance and glioblastoma tumorigenesis based on Genome-scale CRISPR/Cas9 screening
Authors: Sun Zeen , Zeng Feiyue , Liu Yujie , Ouyang Qianying , Tan Jieqiong , Li Xi , Mei Chao , Liu Zhaoqian , Liu Yingzi 
Published: November 2022

Abstract
Introduction: Glioblastoma multiforme (GBM) is a highly aggressive and fatal malignancy of central nervous system. Temozolomide (TMZ) is the first-line chemotherapeutic drug for GBM, although drug resistance remains challenging. To date, MGMT promoter hypomethylation explains part of TMZ resistance cases. The purpose of this study was to utilize the whole human Genome-scale CRISPR-Cas9 knockout (GeCKO) library to screen for novel genes involved in TMZ resistance and explore their functions. Methods: A GeCKO library combined with next-generation sequencing (NGS) was used to screen loss-of-function genes conferring TMZ resistance in GBM cells, then candidate genes were filtrated by qPCR and CCK-8 analysis. Cell proliferation assay, colony formation, wound healing, transwell, and 5-ethynyl-2’-deoxyuridine (EdU) assays were performed to explore the roles of CD99L2 on glioblastoma tumorigenesis in U251 and U87 cell lines. Tumor samples from stage IV GBM patients were used to assess the correlation between CD99L2 expression and progression-free survival (PFS) of the patients. Results: Seven candidate genes including USP17L20, CD99L2, IL2RG, STEAP4, ALKBH3, EDARADD, and PPAPDC3 were identified. Among them, CD99L2 had been confirmed to contribute to TMZ resistance. In U251 and U87 cells treated with TMZ, the knockdown of CD99L2 improved IC50 by 1.39 and 1.54 times compared with the NC groups, respectively. The overexpression of CD99L2 reduced IC50 by 0.52 and 0.58 times compared with the vector groups. CD99L2 knockdown also enhanced tumor proliferation, aggression, vice versa. Moreover, the patients with high-expressed CD99L2 were associated with longer PFS (median PFS: 7.87 months vs. 2.7 months, P = 0.0003). Conclusion: This study found that knocking down CD99L2 could contribute to TMZ-resistance and tumorigenesis in GBM.
Outcomes of the Initial Phase of an Adult Living versus Deceased Donor Liver Transplantation Program in a Low-volume Transplant Center
Authors: Lapisatepun Worakitti , Junrungsee Sunhawit , Chotirosniramit Anon , Udomsin Kanya , Ko-iam Wasana , Lapisatepun Warangkana , Siripongpon Kornpong , Kiratipaisarl Wuttipat , Bhanichvit Pan , Julphakee Thanyathorn 
Published: November 2022

Abstract
Background
The development of living donor liver transplantation (LDLT) is clinically challenging, especially in a low-volume transplant program. We evaluated the short-term outcomes of LDLT and deceased donor liver transplantation (DDLT) to demonstrate the feasibility of performing LDLT in a low-volume transplant/high-volume complex hepatobiliary surgery program during the initial phase.
Methods
We carried out a retrospective study of LDLT and DDLT in Chiang Mai University Hospital from October 2014 to April 2020. Postoperative complications and one-year survival were compared between the two groups.
Results
There were 20 LDLT patients and 20 DDLT patients. The operative time and hospital stay were significantly longer in the LDLT group than in the DDLT group. The incidence of complications in both groups was comparable, except for biliary complications, which were higher in the LDLT group. The most common complication in donor is bile leakage which was found in 3 (15%). The one-year survival rates of both groups were also comparable.
Conclusion
Even during the initial phase of the low-volume transplant program, LDLT and DDLT had comparable perioperative outcomes. Surgical expertise in complex hepatobiliary surgery is necessary to facilitate effective LDLT which has the potential to increase case volumes and lead to program sustainability.
Critical Investigation of Abelmoschus Esculentus (Indian Okra) Fiber Characteristics
Authors: Prafull Prafull P. Kolte , Vijay Vijay S. Shivankar 
Published: November 2022

Abstract
Okra fiber is the bast fiber, extracted from the stem of the Abelmoschus esculentus plant which belongs to the Malvaceae family. In the whole world, India is the largest producer of okra for the cultivation of “okra fruit”, which is one of the main vegetables in the Indian Diet. After harvesting the okra plant on the field, the okra fruit is collected as a vegetable, and the okra plant is thrown out as agricultural waste. The stem of the okra plant is an abundant source of okra fiber. In India, an estimated 45.34 lakh metric tons of green okra plants were discarded annually as agricultural waste which can be used for technical okra fiber extraction. In India, the okra plant has the potential to produce 150-160 Kg of okra fiber per hectare and about 134 lakh Kg okra fiber annually. To prove the suitability of Indian okra fiber (IOF) for commercial applications like jute, flax, hemp, and kenaf fiber this study was formulated. In this study, characteristics of Indian okra fiber (morphological, thermal, and structural) are investigated by optical and electron microscopy (SEM), thermogravimetric analysis (TG, DTG, DSC), X-Ray diffraction analysis (XRD) and Fourier transform infrared spectrometry (FTIR) respectively. Also, the study guides the Indian farmer to generate additional income with the cultivation of the okra plant by extraction of the Indian okra fiber.
Identification of novel mutation in HYAL3 gene of human oligospermia patients by DNA sequencing
Authors: Aslam Sanwal , Zhang Zhen 
Published: November 2022

Abstract
Background
Human male infertility has lot of known molecular components having accurately diagnosis such as Y chromosome deletion and monogenic causes. Only 4% of all infertile male are diagnosed with genetic cases while 60–70% of infertile men still remain without accurate diagnosis and are classified as unexplained. Oligospermia is major cause of human male infertility. Its etiology and pathogenesis are linked with genetic abnormalities. Majority of genetic causes related to human male infertility remains unclear.
Results
Generally, we found significant association among specific type of disease in relation to gender (p = 0.003) and regression value (R2) for this association was 0.75. While type of disease in relation to body mass index (BMI) was not significant (p = 0.34). There was no statistical significant difference (p = 0.40) among types of disease in relation to occupation of patients. Herein we identified novel hyaluronidase 3 (HYAL3) mismatch gene mutation in exon 2 at amino acid 286 position in oligospermia patients who were suffering from primary infertility. The result of this mismatch mutation was the replacement of R (arginine) amino acid with X (any amino acid) in oligospermia patient (R286X). In addition to this, frameshift deletion mutation has also seen just after 25 nucleotide sequences of 2nd mutated exon of HYAL3 gene in oligospermia patients. This frame shift mutation has not seen in secondary infertile patients and in normal individuals. Replacement of R with X has not seen in secondary infertile patients and normal male. In secondary infertile patient we observed the replacement of lysine (K) with serine (S) at 168 position of protein FAST sequence (K168S), lysine (K) with threonine (T)168 position (K168T), histidine (H) with X at 113 position (H113X), glutamine (E) with glutamine(Z)at 164 position (E164Z), proline (P) with X at position 162 (P162X) and phenylalanine (F) with Xat 157 position (F157X).
Conclusion
This study clarifies the site of novel mismatch and frameshift deletion mutation in HYAL3 gene of primary infertile oligospermia patients.
Clinical and Diagnostic characteristics of Non-Alcoholic Fatty Liver Disease among Egyptian children and adolescents with type1 diabetes
Authors: Abdallah Hanaa Reyad , Youness Eman Refaat , Bedeir Manar Maher , Abouelnaga Marwa W. , Ezzat Wafaa M , Elhosary Yasser , El-Hariri Hazem Mohamed , Hussein Mona Abd Elmotaleb , Ahmed Heba R , Eladawy Rasha 
Published: November 2022

Abstract
Background
Type 1 diabetes mellitus (T1DM) patients are at an increased risk for non-alcoholic fatty liver disease (NAFLD). This study aimed to evaluate the clinical criteria associated with the diagnosis of Non Alcoholic Fatty Liver Disease (NAFLD) among T1DM Egyptian children and adolescents.
Methods
74 T1DM patients aged 8–18 y were enrolled in this cross sectional study. Assessments of Clinical status, anthropometric measures, lipid profile, glycated haemoglobin (HbA1c) and liver enzymes were done. Abdominal Ultrasound evaluation of hepatic steatosis was done. Accordingly, patients were divided into two groups (NAFLD & normal liver group) and compared together. Assessment of liver fibrosis using acoustic radiation force impulse elastography (ARFI) was done. Statistical analysis included; independent t-test, Chi square and Fisher’s Exact, Pearson and Spearman tests and Logistic regression models for factors associated with fatty liver were used when appropriate.
Results
in this study; 37 male (50%) & 37 females with mean age 14.3 ± 3.0 y. The mean insulin dose was 1.1 ± 0.4 U/kg & mean disease duration was 6.3 ± 3.0 y. NAFLD was detected in 46 cases while 28 cases had normal liver as diagnosed by abdominal ultrasound. Cases with NAFLD had statistically significant higher BMI-Z scores, waist / hip, waist/ height and sum of skin fold thicknesses compared to those with normal liver (P < 0.05). The mean value of HbA1c % was significantly higher in NAFLD group (P = 0.003). Total cholesterol, triglycerides & LDL serum levels were significantly elevated (p < 0.05), while the HDL level was significantly lower in NAFLD cases (p = 0.001). Although, serum levels of liver enzymes; ALT & AST were significantly higher among cases with NAFLD than in normal liver group (p < 0.05), their means were within normal. Using the ARFI elastography; NAFLD cases exhibited significant fibrosis (F2, 3 &4). BMI, patient age and female gender were among risk factors for NAFLD.
Conclusions
NAFLD represents a serious consequence in type 1 diabetic children and adolescents that deserves attention especially with poor glycemic control. NAFLD has the potential to evolve to fibrosis. BMI, age of patients and female gender were detected as risk factors for NAFLD.
Disruption of resting-state functional connectivity in acute ischemic stroke: comparisons between right and left hemispheric insults
Authors: Katsurayama Marilise , Silva Lucas Scárdua , Campos Brunno Machado , Avelar Wagner Mauad , Cendes Fernando , Yasuda Clarissa Lin 
Published: November 2022

Abstract
Background: Few resting-state functional magnetic resonance imaging (RS-fMRI) studies evaluated the impact of acute ischemic changes on cerebral functional connectivity (FC) and its relationship with functional outcomes after acute ischemic stroke (AIS), considering the side and size of lesions.
Objective: To characterize alterations of FC of patients with AIS by analyzing 12 large-scale brain networks (NWs) with RS-fMRI. Additionally, we evaluated the impact of side (right (RH) or left (LH) hemisphere) and size (lacunar or non-lacunar) of insult on the disruption of brain NWs.
Materials and Methods: 38 patients diagnosed with AIS (19 RH and 19 LH) who performed 3T MRI scans up to 72 hours after stroke were compared to 44 healthy controls. Images were processed and analyzed with the software toolbox UF2C with SPM12. For the first level, we generated individual matrices based on the time series extraction from 70 regions of interest (ROIs) from 12 functional NWs, constructing Pearson’s cross-correlation; the second-level analysis included an analysis of covariance (ANCOVA) to investigate differences between groups. The statistical significance was determined with p<0.05, after correction for multiple comparisons with false discovery rate (FDR) correction.
Results: Overall, individual with LH insults developed poorer six months clinical outcomes. A high degree of FC disruption was observed in LH insults (widespread pattern), mainly in non-lacunar lesions. Changes in FC for RH insults did not survive FDR corrections.
Conclusion: Our findings demonstrated that LH stroke causes severe FC alterations in the network topological properties, presumably related to impairment in their long-term recovery.
A high-capacity dual-ion full battery based on nitrogen-doped carbon nanosphere anode and concentrated electrolyte
Authors: Yuan Wenhui , Wu Hongzheng , Luo Shenghao , Li Li 
Published: November 2022

AbstractDual-ion batteries (DIBs) are often criticized for their low discharge capacity and poor cyclic capability despite their inherent high working voltage, low manufacturing cost and environmental friendliness. To solve these shortcomings, many attempts and efforts have been devoted, but all ended in unsatisfactory results. Herein, a hierarchical porous carbon nanosphere anode with high nitrogen doping is developed, which exhibits fast ion transport kinetics and excellent Li+storage capability. Moreover, employing concentrated electrolyte is expected to bring a series of advantages such as stable SEI for facilitating ion transmission, enhanced cycling performance, high specific capacity and operation voltage. These advantages endow the assembled full DIBs with excellent performance as a super-high specific discharge capacity of 351 mAh g− 1and can be cycled stably for 1300 cycles with Coulombic efficiency (CE) remaining at 99.5%; a high operating voltage range of 4.95–3.63 V and low self-discharge rate of 2.46% h− 1with stable fast charging-slow discharging performance. Through electrochemical measurements and physical characterizations, the possible working mechanism of the proof-of-concept full battery and the structural variations of electrodes during cycling are investigated. The novel battery system design strategy in this work will promote the development of high-performance DIBs.
Hydrogen gas ameliorates the LPS-induced BPD via inhibiting the activation of TNF-α/NF-κB inflammatory signaling pathway in placenta
Authors: Zhang Yafang , Ren Xianhui , Zhang Linli , Jing Xiujie , Chen Yunxi , Tian Yan , Chu Zhongxia , Yao Guo , Wang Yan 
Published: November 2022

Abstract
Objective
To investigate the anti-inflammatory role of H2 in LPS-induced BPD via regulating TNF-α/NF-κB signaling pathway in placenta.
Methods
We induced a neonatal rat model of BPD by injecting lipopolysaccharide (LPS, 1ug) into the amniotic fluid at embryonic day 16.5(E16.5). Treatment of 30% hydrogen gas for 4 hours/day with continuously 5days. We primarily analyzed the neonatal outcomes and then compared inflammatory levels from Control group (CON), LPS group (LPS) and LPS with H2 inhalation group (LPS + H2). TUNEL and Hematoxylin-Eosin (HE) staining were performed to evaluate inflammatory and apoptotic levels. We further used RNA sequencing and ELISA assay to examine differentially expressed proteins and mRNA levels of tumor necrosis factor-α (TNF-α), nuclear factor kappa-B (NF-κB) (p65), interleukin (IL)-6, IL-18, IL-1β, C-C motif chemokine ligand 2(CCL2) and C-X-C motif chemokine ligand 1(CXCL1). Bioinformatics analysis (GO and KEEG) of RNA-seq and correlation analysis were applied to clarify the mechanisms of H2 anti-inflammatory effect on LPS-induced BPD.
Results
We found the H2 inhalation decreased production of inflammatory cytokines/chemokines (IL-6, IL-18, IL-1β, CCL2, CXCL1) in LPS-induced placenta to rescue from the BPD. Upon administration of H2, infiltration degree of LPS-induced placenta was reduced and infiltrating significantly narrowed down. Hydrogen normalized LPS-induced perturbed lung development, reduced lung apoptotic index, death ratio of fetus and neonate. Meanwhile, H2 also upregulated the survival ratio. RNA-seq and Elisa demonstrated that both mRNA and protein levels of TNF-α/NF-κB signaling pathway were activated by LPS, and H2 relieved the pro-inflammatory function of LPS on TNF-α/NF-κB-stimulated placenta. Correlation analysis showed a positive association of TNF-α vs both NF-κB and inflammatory cytokines/chemokines.
Conclusion
H2 inhalation alleviated LPS-induced BPD by inhibiting excessive pro-inflammatory cytokines and inflammatory chemokines via the TNF-α/NF-κB signaling pathway in placenta and may be a potential therapeutic strategy for BPD.
A sytematic study of covid-19 prediction models of India
Authors: Yadav Ameet , Rana Chhavi 
Published: November 2022

Abstract
Infecting billions of people and death of over 6.5 millions people and loss of economy across the world, this COVID-19 outbreak caused by SARS-COV-2 has uncover the poor health management in the large populated country like India. Therefore, there is a requirement of detailed study i.e. Systematic Literature Review(SLR) of existing models by analysing the predicting behaviour of epidemic which plays a vital role in controlling the spread in future. Our study considered prediction models of COVID-19 which includes case study of India using machine learning and deep learning. This study includes only scholarly peer-review research articles of two renowned databases, Web of Science and Scopus from 2020–2022. PRISMA(Preferred Reporting Items for Systematic Reviews and Meta Analysis) guidelines have used for results and discussion. Before screening 317 articles were reported and after screening, eligibility of inclusion/exclusion criteria, 51 research articles were included for the final study. This SLR examined articles thoroughly, identified different machine learning, deep learning prediction models, identified research gaps/limitations, future scopes, and examined different performance metrics used in these studies. An additional objective of this research is to identify limitations and future directions provided by each research article which were not covered in any SLR on COVID-19.
Evaluation Of The Arterial Folding Point During Flexion Of The Hip: about 37 dynamic angiographies
Authors: MARTIN Théodore , MATHY Marie , FEUGIER Patrick , VISTE Anthony 
Published: November 2022

Abstract
Purpose
The aim was to determine the location of the folding point of the arterial ilio-femoral axis and his angulation.
Materials and methods
Forty-five dynamic arteriographies were analyzed and 37 were included in the current study. Different lengths were measured, based on specific anatomical landmarks: the departure of the external iliac artery, the inguinal ligament and the bifurcation of the femoral artery. These lengths were measured in extension and during flexion of the hip in order to determine the folding point.
Results
In extension, some physiological angulations of the external iliac artery were measured.
During flexion of the hip joint, the distance from the folding point to the bifurcation of the common iliac artery was respectively 82 +/- 21 mm (range 48-116) on the right and 95 +/- 20 mm (range 59-132) on the left side. The distance from the folding point to the inguinal ligament was respectively 38 +/- 40 mm (range 12-138) on the right and 26 +/- 23 mm (range 8-136) on the left side. The distance from the folding point to the bifurcation of the femoral artery was respectively 45 +/- 29 mm (range 15-107) on the right and 27 +/- 12 mm (range 10-66) on the left side. During flexion, the angulation of the folding point of the ilio-femoral axis was 114 +/- 18 ° (range 81-136°).
Conclusions
The folding point was located cranially to the inguinal ligament and below the departure of the external iliac artery.
A facile approach for preparing nanofabricated cellulose from bleached corn stalk with tailored surface functions
Authors: Tian Jiarong , Qian Shaoping , Chen Jing , Zhang Zhaoyan , Wan Yi 
Published: November 2022

Abstract
This study proposed a facile, efficient, and energy-saving one-pot strategy to produce nanofabricated cellulose (NFC) from bleached corn stalk along with concurrent modifications of the surface. High-speed mechanical shearing and mechanochemical activation (urea/NaOH, oxalic acid, citric acid, and mercaptopropyl trimethoxysilane) were involved in the strategy. The effects of different surface functionalization on the morphological, crystalline, chemical, and thermal properties of the NFC were investigated. The results suggested that the bleached corn stalk fibers were separated into microfibrils associated with abundant branched and networked cellulose nanofibrils through intensive mechanical shearing. NFC showed an average diameter of 23 nm and an average length of 1530 nm, with an aspect ratio of 67. The hydroxyl groups on the NFC surface were functionalized and modified by forming amide (urea/NaOH), carboxyl (oxalic acid/citric acid), and hydrogen bond (mercaptopropyl trimethoxysilane), respectively. This novel one-pot strategy could open up new application areas for production and surface functionalization of nanocellulose.