bluuebunny/crossref_metadata_2025_split huggingface dataset:
TNFAIP6 Defines the MSC Subpopulation with Enhanced Immune Suppression Activities
Authors: Li Lingyun , Chen Xiangjuan , Chen Xian , Yang Lei , Zeng Yong , Xu Jianyong 
Published: March 2022

Abstract
Background: Mesenchymal stem cells (MSCs) have been intensively investigated in both pre-clinical and clinical studies. However, the therapeutic efficacy varies resulting from the heterogenicity of MSCs. Therefore, purifying the specific MSC subpopulation with specialized function is necessary for their therapeutic applications. Methods: The large-scale RNA sequencing analysis was performed to identify potential cell markers for the mouse MSCs. Then the immune suppression activities of the purified MSC subpopulation were assessed in vitro and in vivo. Results: The TNFAIP6 (tumor necrosis factor alpha induced protein 6) has been identified as a potential cell marker for mouse MSCs, irrespective of tissue-origin and lab-origin. The TNFAIP6+ mouse MSCs showed enhanced immune suppression activities and improved therapeutic effects on the mouse model of acute inflammation, resulting from faster response to immune stimulation. Conclusions: Therefore, we have identified TNFAIP6 as a new MSC marker and the TNFAIP6+ MSC subpopulation has enhanced immune suppression capabilities.
Paraneoplastic Hyperthyroidism Secondary to a Chemotherapy-Induced Surge in β-hCG in a Patient with Non-Seminomatous Germ Cell Tumor: Case Report and Literature Review
Authors: Erol Lindsay , Layman Clifton 
Published: March 2022

Abstract
Background: Thyrotoxicosis as the presenting syndrome of an underlying β-hCG-secreting malignancy is well described. It has been previously theorized, but not reported, that the surge of β-hCG secondary to chemotherapy induction may inadvertently trigger thyrotoxicosis. To our knowledge, this is the first documented case of such event in peer-reviewed medical literature published in the English language. Case presentation: We present a case of a 21-year-old male with stage IIIc non-seminomatous germ cell tumor who developed paraneoplastic hyperthyroidism within four days of his first cycle of chemotherapy. Successful treatment of his hyperthyroidism was achieved with methimazole, atenolol and continued treatment of his malignancy. Conclusions: We suggest management considerations based on our case and review of the literature.
Towards real-time probabilistic ash deposition forecasting for Aotearoa New Zealand
Authors: Trancoso Rosa , Behr Yannik , Hurst Tony , Deligne Natalia 
Published: March 2022

Abstract
Volcanic ashfall forecasts are highly dependent on eruption parameters and synoptic weather conditions at the time and location of the eruption. In Aotearoa, New Zealand, MetService and GNS Science have been jointly developing an ashfall forecast system that incorporates 4D high-resolution numerical weather prediction (NWP) and eruption parameters into the HYSPLIT model, a state-of-the art hybrid Eulerian and Lagrangian dispersion model widely used for volcanic ash. However, these forecasts are based on discrete eruption parameters combined with a deterministic weather forecast and thus provide no information on output uncertainty. This shortcoming hinders stakeholder decision making, particularly near the geographical margin of forecasted ashfall and in areas with large gradients in forecasted ash deposition. This study presents a new approach that incorporates uncertainty from both eruptive and meteorologic inputs to deliver uncertainty in the model output. To this end, we developed probabilistic density functions (PDFs) for the three key eruption parameters (plume height, mass eruption rate, eruption duration) tailored to Aotearoa’s volcanoes, and combine them with NWP ensemble datasets to generate probabilistic ashfall forecasts using the HYSPLIT model. We show that the Latin Hypercube Sampling (LHS) technique can be used to representatively span this high-dimensional parameter space with fewer model runs than Monte Carlo techniques, thus allowing this methodology to be used in near real-time forecast systems. We also propose new probabilistic summary products such as hazard matrices, probability of exceedance of cumulative ashfall, and arrival time forecasting, which together support public information and emergency responders decision making.
Chemical investigation and Antibacterial Activity of Leaves Extracts of Artemisia absinthium
Authors: Ewunu Sitotaw , Awoke Sisay , Getachew Mesfin 
Published: March 2022

Abstract
Plants are among the most important common sources of potentially valuable new drugs. Therefore, to investigate biological activity of medicinal plants are used to develop new drugs. Artemisia absinthium L. is an aromatic plant of the family Asteraceae, and is known by the common names wormwood and absinthe. The study of this research was aimed to chemical investigation, essential oil analysis and antibacterial activity of A. absinthium. The plant was collected in different local area of north Wollo, Woldiya. Maceration technique was used for extraction. Isolation of essential oil was done using Cleavinger apparatus. Column chromatography was used to isolate major compounds. In this present study, the major plant secondary metabolites present in A. absinthium was screened, the major constituents the plant was investigated and antibacterial activity of the crude extracts was performed. The results of phytochemical screening showed the presence of alkaloid, flavonoid, carbohydrate, tannin, quinines, protein, phenol and terpenoids. The identified components account for 56% of the oil. The oil contains camphor as a major constituent and account for 41% of the oil. From column chromatography two compounds, chamazulene and davanone were isolated and identified on the bases of their 1H and 13C NMR spectra. Antimicrobial assay of the crude extracts of A. absinthium against gram negative (E. coli and K. pneumonia) and gram positive (L. monocytogen and S. aureus) pathogenic microorganisms showed that a significance activity. Different concentrations of methanol and chloroform were tested and maximum zone of inhibition was found at 200mg/ml in both extracted and zone of inhibition was decreased with decreasing concentrations.
Prevalence of Preterm Premature Rupture of Membrane and Associated Factors Among Pregnant Women Admitted To Health Facilities in Ambotown, Ethiopia, 2021.
Authors: Gutema Rebuma Muleta , Dina Gurmesa Daba , Berhanu Anisa , Erena Maru Mossisa 
Published: March 2022

Abstract
Background: Preterm pre-labor rupture of the membrane is a major cause of perinatal, neonatal, and maternal morbidity and mortality both in high- and low-income countries. A woman with premature rupture of membranes is at risk of complications like intra-amniotic infection, postpartum hemorrhage, and death. Little is known about the problem in the study area, therefore, this study was designed to determine the prevalence of preterm premature rupture of membrane and its associated factors among pregnant women admitted to health facilities in ambo town. Methods: Hospitals based cross sectional study was conducted on 391 pregnant women who were admitted to the Hospitals in Ambo town from July 05/2021-August 30/2021.The data were collected through face-to-face interviews by a structured questionnaire. Descriptive analyses were performed using frequencies, percentages, binary logistic regression by adjusting for confounding factors.Results: The prevalence of preterm premature rupture of membrane was found to be 22.6%. Preeclampsia (AOR=3.2, 95% CI=0.69-0.57), Economic status (AOR = 2.64 (95% CI = 1.99–6.01), current urinary tract infection (AOR = 2.42, 95% CI = 1.32–5.19), previous history of premature rupture of membrane (AOR = 2.31, 95% CI = 1.02–6.27), andanemia (AOR = 1.85, 95% CI = 0.65–4.56) were factors associated with preterm premature rupture of membrane.Conclusions: The prevalence of preterm premature rupture of membrane in the study area was high. Based on the predisposing factors identifiedearly screening and treatment as well as health promotion isimportant to reduce the risk of preterm premature rupture of membrane.
Factors Leading to Disruptive Behaviours Involving Radiographers: A Qualitative Study
Authors: Chinene Bornface , Nkosi Busisiwe Pauline , Sibiya Maureen Nokuthula 
Published: March 2022

Abstract
Background
The negative consequences of disruptive behaviours involving healthcare workers threatens the institution’s image, staff morale, finances, and patient safety. While all kinds of health organisations or settings are potentially exposed to disruptive behaviours, some are at higher risk than others because of both environmental and cultural factors. Such risk should be assessed having regard to the specific situation and conditions in which each healthcare organization operates. International regulatory best-practice standards now require healthcare institutions to develop policies and plans at specific workplaces to combat DBs and establish the required monitoring mechanisms and a range of sanctions. The purpose of this study was to explore the environmental and cultural factors leading to DBs involving radiographers at central hospitals in Harare Metropolitan Province so that tailor-made strategies for addressing these behaviours can be formulated.
Methods
An exploratory qualitative study employing face to face, one-on-one in-depth interviews was carried out between March and April 2021. Eleven radiography managers at central hospitals in Harare Metropolitan Province (HMP) were selected by criterion purposive sampling. The interview data were analysed using Tesch's method of qualitative analysis. The data was first manually coded and then entered into Nvivo (QSR International Version 11) for further analysis. This study was assessed using the four criteria for developing the trustworthiness of qualitative research as suggested by Lincoln and Guba, namely credibility, dependability, conformability and transferability.
Results
The key environmental and cultural factors identified included three themes: power hierarchy, work environment and reporting framework. Power hierarchy comprised categories, superiority, professional boundaries and representation. The work environment incorporated categories, trust in leadership, burnout and fatigue and remuneration. Lastly, reporting framework included categories, lack of protocol and reporting culture.
Conclusions
This study highlights that environmental factors play a bigger role in provoking disruptive behaviours involving radiographers at central hospitals in HMP. This underscores the need for hospital and radiography managers to pay particular emphasis on these when formulating policies and procedures to address these behaviours. Addressing DBs ensures that healthy work environments are promoted which in turn ensures that patients receive optimum and safe care.
Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications
Authors: El-Adili Fatima , Lui Justin K. , Najem Mortada , Farina Giuseppina , Trojanowska Maria , Sam Flora , Bujor Andreea M. 
Published: March 2022

Abstract
Objective
To evaluate the levels of periostin in patients with systemic sclerosis (SSc) and their association with features of systemic sclerosis.
Methods
The levels of periostin were assessed in the serum of 106 SSc patients and 14 healthy controls, and by immunofluorescence staining in cardiac tissue from 4 SSc patients and 4 healthy controls. Serum periostin was measured via enzyme-linked immunosorbent assay, and the natural logarithm of periostin (log-periostin) was calculated. Results were analyzed using unpaired t-test or one-way ANOVA followed by Tukey’s multiple comparisons tests, and Pearson’s test for correlations. Cardiac tissue from SSc patients and controls was stained for periostin and co-stained for periostin and collagen type I using immunofluorescence.
Results
Periostin levels were higher in patients with SSc compared to controls, and directly correlated to modified Rodnan skin score and echocardiography parameters of left ventricular measurements. Immunofluorescence staining in SSc cardiac tissue showed patchy periostin expression in all SSc patients, but not in controls. Furthermore, there was extensive periostin expression even in areas without collagen deposition, while all established fibrotic areas showed co-localization of collagen and periostin. There was no association between periostin levels and interstitial lung disease, pulmonary hypertension or other vascular complications.
Conclusion
Periostin is elevated in SSc cardiac tissue in vivo and circulating levels of periostin are increased in SSc, correlating with the extent of disease duration, degree of skin fibrosis, and left ventricular structural assessments. Periostin may be a potential biomarker that can provide further pathogenic insight into cardiac fibrosis in SSc.
COVID-19 testing: A qualitative study exploring enablers and barriers in the Greater Accra Region, Ghana
Authors: Ha Solip , Yangchen Sonam , Assan Abraham 
Published: March 2022

Abstract
Introduction: The COVID-19 pandemic is a global crisis and has reaffirmed that extensive testing along with effective tracing and treatment is still crucial to slowing transmission of the COVID-19 virus despite the rolling out of vaccines. This study explored enablers and barriers to COVID-19 testing in the Greater Accra region of Ghana. We envision lessons learned from this study could serve as a roadmap to strengthen the current response to COVID-19 and preparedness for future outbreaks, particularly in low- and middle-income countries.Methods: A qualitative design was undertaken to explore the phenomenon. Data collection methods included in-depth interviews with key informants with a purposively selected sample of 20 participants. Interviews were conducted using flexible semi-structured interview guides. Depending on the participant's position and involvement in COVID-19 testing, the guides were modified, and key elements were drawn from a tailored version of the WHO Health System Framework incorporating the Essential Public Health Functions (EPHF). The interview findings were augmented by reviewing published literature.Results: Better health governance through political leadership, community participation, multisectoral collaboration, effective resource management and information systems played a crucial role in catalyzing COVID-19 testing. The primary barriers to testing were mainly COVID-19 infodemic, inadequacy of material resources to meet growing health needs, and lack of opportunities to have equal and easy access to testing services. Further, although human resources were adequate, they were unevenly distributed across settings.Conclusion: Despite rolling out vaccines against COVID-19, testing remains an important measure to control the virus. To effectively be prepared for extensive COVID-19 testing and respond to future outbreaks, the following are recommended: there should be improved political commitments, coordination and communication with diverse actors to ensure even distribution of all resources across the country; empowerment of community members should be encouraged to develop community-oriented pandemic preparedness and management of COVID-19 infodemic; investment in strengthening capacity of Good Manufacturing Practice (GMP) is needed to stimulate diversification into health products.
Molecular Subtyping of Invasive Breast Carcinoma by Immunohistochemistry and Five-Year Survival Study
Authors: Vipparla Raja Silvan , Naidu Raji Tejas , Cherian Susan , Shettigar Suresh S 
Published: March 2022

Background: Global gene expression profiling for Invasive breast carcinoma (IBC) has identified intrinsic subtypes of IBC with differing clinical outcomes and response to therapy. As genotyping assays are limited by availability and cost, we have used Immunohistochemistry (IHC) surrogates to classify IBC into molecular subtypes.
Methods: Representative tumor blocks of 158 surgical specimens of IBC between 2007 to 2017, were selected and IHC done for ER, PR, Her2, Ki67, CK 5/6 and EGFR. The cases were classified into 7 Molecular subtypes (Luminal A, Luminal B, Luminal HER2PR+, Luminal HER2PR-, HER2Enriched, Basal like (BLBC) and non classifiable (NCBC) and correlated with clinico-pathological findings. Five- year survival rate was calculated for patients diagnosed between 2007 to 2013.
Result: The most common subtype was Luminal A (31.0%), followed by Luminal B (25.3%), NCBC (14.6%) and HER2 enriched (13.3%). Among post-menopausal women, common subtypes were Luminal A (33.8%) and Luminal B (24.4%). Among premenopausal women, most cases were NCBC (27.8%) and BLBC (22.2%). 61.2% of Luminal A were Grade2 and 22.4% were Grade 1. Many cases of Luminal B and HER2 positive cases were of Grade3 (45.0%) and (57.1%) respectively. Of the triple-negative category (BLBC & NCBC), 73% were Grade3 with statistically significant correlation (p value < 0.001). Most of these cases were in Tumor stage T2 (70%), followed by T1 (22.3%). Nodal metastasis was seen in 39.6% and 65% respectively of Luminal A and Luminal B subtypes. Distant metastases on follow-up were present in 15.8%, which included HER2 enriched subtype (28.5%), followed by BLBC (20.0%) and NCBC (17.4%). Luminal A cases, had better survival accounting for 88% of all survivors.
Conclusion: Molecular subtyping of IBC using IHC was useful to understand the clinicopathological distinctiveness of each subtype.
Turismo religioso em Lunardelli, no Vale do Ivaí
Authors: Costa de Queiroz DAntonio Giulia , de Oliveira Domingos Fabiane 
Published: March 2022

A motivação principal para o turismo religioso está ligada a espiritualidade, mas pessoas não religiosas também praticam esta modalidade do turismo, pela busca de novas experiências e conhecimento. Lunardelli é um município novo e pequeno, localizado na região do Vale do Ivaí, no Paraná. Antes da pandemia da Covid-19, estava recebendo um aumento no fluxo de visitantes por conta do Santuário Santa Rita de Cássia, conhecida como a Santa das causas impossíveis. O município se destaca como um importante destino de turismo religioso, certificado como a Capital Paranaense da Fé. Nesse sentido, essa pesquisa tem por objetivo analisar os impactos socioeconômicos do turismo religioso e alguns desses impactos causados pela pandemia na atividade turística do município. Nesta pesquisa qualitativa foi realizada a análise bibliográfica e pesquisas de campo com visitas técnicas para a coleta de dados. Isso ocorreu antes e durante a pandemia, sendo possível tecer algumas considerações nas áreas de meio ambiente, planejamento urbano, cultura e economia. Desse modo podemos constatar que, por conta da importância do turismo no município, é preciso solucionar os principais problemas decorrentes da atividade, já que o turismo religioso se iniciou de forma desordenada. Com o devido planejamento, espera-se a continuidade das ações implementadas pelo setor público, pelo Santuário e pela iniciativa privada, com o retorno pleno das atividades pós-vacina.
Palavras-chave: Planejamento Turístico. Desenvolvimento Local. Turismo.
The Immune Response to SARS-CoV-2: Mechanisms, Aging, Sequelae, and Vaccines
Authors: Alarcón Marcelo , Espinoza Carolina 
Published: March 2022

Abstract:
This review seeks to clarify the factors involved in the various immune responses to SARSCoV- 2 infection and the mechanisms that influence the development of COVID-19 with severe evolution. The innate immune response that evolves against SARS-CoV-2 in a complex way is highlighted, integrating multiple pathways by coronaviruses to evade it, in addition to characterizing the adaptive immune response, which can lead to an effective immune response or can contribute to immunopathological imbalance. In turn, host-dependent biomarkers, such as age, gender, ABO blood group, and risk factors, that contribute to the critical and varied progress of COVID-19 immunopathogenesis are analyzed. Finally, the potential vaccine candidates are presented, capable of generating immune protection with humoral and/or cellular neutralizing responses, in favor of blocking and destroying both the new human coronavirus and its variants, which cause the current pandemic
Pengembangan Media Video Fenomena Predasi Insektivora dalam Pembelajaran Biologi Secara Daring di SMA PGRI 2 Kota Jambi
Authors: Amrulia Risma Hesy , Johari Asni , Murni Pinta 
Published: March 2022

Learning media in the form of video is a medium that can be used by teachers to be used in learning. Video media contains learning materials that are equipped with pictures and videos that make it easier to understand the material. This study aims to develop learning media in the form of videos, determine the feasibility, responses of teachers, students, and the effectiveness of video media that have been developed to improve learning outcomes. Video media development uses the ADDIE model, which consists of the analysis, design, development, implementation, and evaluation stages. Video media products are validated by media experts and material experts with excellent results. The results of the biology teacher's response as well as small group and large group trials obtained very good results. The results of the effectiveness test using the paired-sample t test showed that there was an effect on the use of video media on the insectivorous predation phenomenon in improving student learning outcomes. Based on the results obtained, it is concluded that the media developed is suitable for use in learning.
Key words: Video Media, Online Learning, Insectivorous Predation Phenomen

Abstrak. Media pembelajaran berupa video merupakan media yang bisa dipakai oleh guru untuk digunakan dalam pembelajaran. Media Video memuat materi pembelajaran yang dilengkapi dengan gambar serta video yang memudahkan dalam memahami materi. Penelitian ini bertujuan untuk mengembangkan media pembelajaran berupa video, mengetahui kelayakan, respon guru, peserta didik, serta efektivitas media video yang telah dikembangkan dalam meningkatkan hasil belajar. Pengembangan media video menggunakan model ADDIE yang terdiri dari tahap analysis, design, development, implementation dan evaluation. Produk media video divalidasi oleh ahli media dan ahli materi dengan perolehan hasil sangat baik. Hasil respon guru biologi serta ujicoba kelompok kecil dan kelompok besar diperoleh hasil sangat baik. Hasil uji efektivitas menggunakan uji paired sample t test menunjukkan terdapat pengaruh pada penggunaan media video pada materi fenomena predasi insektivora dalam meningkatkan hasil belajar peserta didik. Berdasarkan hasil yang diperoleh maka disimpulkan bahwa media yang dikembangkan layak untuk digunakan dalam pembelajaran.
Kata kunci: Media Video, Pembelajaran Daring, Fenomena Predasi Insektivora