bluuebunny/crossref_metadata_2025_split huggingface dataset:
Herbaceous eudicot Fairlingtonia from the Lower Cretaceous of Jiuquan Basin, Northwest China and its radiation in Laurasia
Authors: Du Bao‐Xia , Zhang Ming‐Zhen , Zhang Jing , Li Ai‐Jing , Lin Shao‐Hua , Ma Guo‐Rong , Hui Jian‐Guo 
Published: November 2022

AbstractEudicots exhibit diverse life forms and occupy a wide variety of habitats in the modern terrestrial ecosystems, and the diversification began during the Early Cretaceous; however, few Early Cretaceous fossils are preserved as multiorgan whole plants that can provide sufficient morphological characters for detailed phylogenetic assessment. Here, Fairlingtonia microgyna sp. nov. is reported from the upper Lower Cretaceous of Zhonggou Formation, Hanxia Section, Yumen City, western Gansu Province, Northwest China. The specimen is exceptionally preserved as multiorgan whole plant fossil with fibrous adventitious roots, simple and deeply dissected leaves, solitary and dehiscent capsular fruits attached to the creeping stems. As such, it was interpreted as a herbaceous eudicot. Phylogenetic analyses support a placement within the Papaveraceae, most likely in Papaveroideae, but there are obvious differences in morphological characteristics, which cannot confirm the systematic position within the Papaveraceae. Fossil records of Fairlingtonia from contemporaneous deposits (late Aptian to early Albian) in Northwest China and eastern North America provide direct evidence of the geographical radiation of Fairlingtonia on Laurasia. And the morphological characters of F. microgyna, including creeping leafy branches, fibrous adventitious roots, small and deeply dissected leaves as well as small capsular fruits with tiny seeds probably indicate that it was a colonizer of lake‐shore environments under wet and bright conditions and possessed fast‐growing and rapid propagation habitats, which allowed it to expand its geographic range with both sexual and asexual reproduction.
A glutathione‐independent DJ‐1/PfpI domain‐containing tomato glyoxalaseIII2, SlGLYIII2, confers enhanced tolerance under salt and osmotic stresses
Authors: Gambhir Priya , Singh Vijendra , Raghuvanshi Utkarsh , Parida Adwaita Prasad , Pareek Amit , Roychowdhury Abhishek , Sopory Sudhir K. , Kumar Rahul , Sharma Arun Kumar 
Published: November 2022

AbstractIn plants, glyoxalase enzymes are activated under stress conditions to mitigate the toxic effects of hyperaccumulated methylglyoxal (MG), a highly reactive carbonyl compound. Until recently, a glutathione‐dependent bi‐enzymatic pathway involving glyoxalase I (GLYI) and glyoxalase II (GLYII) was considered the primary MG‐detoxification system. Recently, a new glutathione‐independent glyoxalase III (GLYIII) mediated direct route was also reported in plants. However, the physiological significance of this new pathway remains to be elucidated across plant species. This study identified the full complement of 22 glyoxalases in tomato. Based on their strong induction under multiple abiotic stresses, SlGLYI4, SlGLYII2 and SlGLYIII2 were selected candidates for further functional characterisation. Stress‐inducible overexpression of both glutathione‐dependent (SlGLYI4 + SlGLYII2) and independent (SlGLYIII2) pathways led to enhanced tolerance in both sets of transgenic plants under abiotic stresses. However, SlGLYIII2 overexpression (OE) plants outperformed the SlGLYI4 + SlGLYII2 OE counterparts for their stress tolerance under abiotic stresses. Further, knockdown of SlGLYIII2 resulted in plants with exacerbated stress responses than those silenced for both SlGLYI4 and SlGLYII2. The superior performance of SlGLYIII2 OE tomato plants for better growth and yield under salt and osmotic treatments could be attributed to better GSH/GSSG ratio, lower reactive oxygen species levels, and enhanced antioxidant potential, indicating a prominent role of GLYIII MG‐detoxification pathway in abiotic stress mitigation in this species.
Report of a case of RAVEN, hair heterochromia and autism in the setting of FGFR2 mutation
Authors: Gracia‐Darder Inés , Llull Ramos Ana , Giacaman Aniza , Gómez Bellvert Cristina , Obrador‐Hevia Antonia , Jubert Esteve Elisabeth , Martín‐Santiago Ana 
Published: November 2022

AbstractA newborn presented with extensive rounded and velvety epidermal nevus (RAVEN) with a genetic study of the cutaneous lesions revealing a heterozygous mutation in FGFR2 (p.Cys382Arg). By 2 years of age, the patient developed hair heterochromia and autism spectrum disorder. Although RAVEN was initially associated with fibroblast growth factor 3 (FGFR3) mutations, three cases of RAVEN have been identified with mutations in FGFR2 (p.Ser252Trp) and one case of linear keratinocytic epidermal nevi has been identified with the same mutation as the mutation identified in our patient. This strongly supports the pathogenic role of these mutations.
Genome‐wide identification and expression analysis of NAC family genes in Ginkgo biloba L.
Authors: Li Y. , Han H. , Fu M. , Zhou X. , Ye J. , Xu F. , Zhang W. , Liao Y. , Yang X. 
Published: November 2022

ABSTRACT

NAC (NAM, ATAF, CUC2) transcription factors constitute one of the largest families of plant‐specific transcription factors with important roles in plant growth and development and in biotic and abiotic stresses.

The physicochemical properties, gene structure, cis‐acting elements and expression patterns of NAC transcription factors in Ginkgo biloba were analysed using bioinformatics, and expression of this gene family was analysed via quantitative reverse transcription PCR.

The family of G. biloba NAC transcription factors had 50 members, distributed on 12 chromosomes and divided into 11 groups. Members in the same group share a similar gene structure and motif distribution. Transcriptome data analysis of G. biloba showed that 35 genes were expressed in eight tissues. Correlation analysis suggested that GbNAC007 and GNAC008 might be involved in flavonoid biosynthesis. Expression levels of 12 GbNACs under cold, het, and salt stresses were analysed. Results indicate that NAC transcription factors play an important role in response to abiotic stresses.

This study provides a reference for the functional analysis of the G. biloba family of NAC transcription factors, as well as a resource for studies on the involvement of this family in responses to abiotic stresses and flavonoid biosynthesis.
Anisohydric characteristics of a rice genotype ‘ARC 11094’ contribute to increased photosynthetic carbon fixation in response to high light
Authors: Taniyoshi Kazuki , Tanaka Yu , Adachi Shunsuke , Shiraiwa Tatsuhiko 
Published: November 2022

AbstractPhotosynthetic induction, which is the response of the CO2 assimilation rate to a stepwise increase in light intensity, potentially affects plant carbon gain and crop productivity in field environments. Although natural variations in photosynthetic induction are determined by CO2 supply and its fixation, detailed factors, especially CO2 supply, are unclear. This study investigated photosynthesis at steady and non‐steady states in three rice (Oryza sativa L.) genotypes: ARC 11094, Takanari and Koshihikari. Stomatal traits and water relations in the plants were evaluated to characterise CO2 supply. Photosynthetic induction in ARC 11094 and Takanari was superior to that in Koshihikari owing to an efficient CO2 supply. The CO2 supply in Takanari is attributed to its high stomatal density, small guard cell length and extensive root mass, whereas that in ARC 11094 is attributed to its high stomatal conductance per stoma and stomatal opening in leaves with insufficient water (i.e., anisohydric stomatal behaviour). Our results suggest that there are various mechanisms for realising an efficient CO2 supply during the induction response. These characteristics can be useful for improving photosynthetic induction and, thus, crop productivity in field environments in future breeding programmes.
LncRNA‐mediatedceRNAregulatory network provides new insight into chlorogenic acid synthesis in sweet potato
Authors: Peng Ying , Pan Rui , Liu Yi , Medison Milca Banda , Shalmani Abdullah , Yang Xinsun , Zhang Wenying 
Published: November 2022

AbstractSweet potato (Ipomoea batatasL.) is considered a highly nutritional and economical crop due to its high contents of bioactive substances, such as anthocyanin and chlorogenic acid (CGA), especially in leaves and stems. The roles of noncoding RNAs (ncRNA), including long noncoding RNA (lncRNA) and microRNA (miRNA), in CGA synthesis, are still unknown. In this study, the differentially expressed (DE) mRNAs, miRNAs, and lncRNAs in two leafy vegetable genotypes “FS7‐6‐14‐7” (high CGA content) and “FS7‐6” (low CGA content) were identified. The cis‐regulation between lncRNA and mRNA was analyzed. Then, the CGA synthesis‐related modules MEBlue and MEYellow were identified to detect trans‐regulation mRNA‐lncRNA pairs. The GO and KEGG annotations suggested that mRNA in these two modules was significantly enriched in the secondary metabolite synthesis biosynthesis category. A competing endogenous RNAs (ceRNA) network, including 8730 miRNA‐mRNA and 444 miRNA‐lncRNA pairs, was constructed by DEmiRNA target prediction. Then, a CGA synthesis‐related ceRNA network was obtained with lncRNA and mRNA from MEBlue and MEYellow. Finally, one relational pair,MSTRG.47662.1/mes‐miR398/itb04g00990, was selected for functional validation. Overexpression of lncRNAMSTRG.47662.1and mRNAitb04g00990increased CGA content in both tobacco and sweet potato callus, while overexpression of miRNAmes‐miR398decreased CGA content. Meanwhile, regression analysis of the expression patterns demonstrated thatMSTRG.47662.1, acting as a ceRNA, promoteditb04g00990expression by competitively bindingmes‐miR398in CGA synthesis in sweet potato. Our results provide insights into how ncRNA‐mediated ceRNA regulatory networks likely contribute to CGA synthesis in leafy sweet potato.
Holton et al., Characterization of asbestos exposures associated with the use of facial makeups. Risk Analysis, 42, 2129–2139
Authors: Finkelstein Murray M. 
Published: November 2022

AbstractHolton and colleagues have performed a risk assessment after measuring asbestos released from several samples of facial makeup. Unfortunately, it is not possible to interpret or generalize their findings because the authors have not described the source(s) of the talc tested or the asbestos concentrations of the samples. The concentration of amphiboles varies widely between sources, and the authors are urged to divulge the locations of the ore bodies providing the talc for their samples, as well as the asbestos concentration of the samples, so that the results may be interpreted and possibly generalized.
Red blood cell alloimmunisation in sickle cell disease patients in the Democratic Republic of the Congo
Authors: Kambale‐Kombi Paul , Djang'eing'a Roland Marini , Alworong'a Opara Jean‐Pierre , Minon Jean‐Marc , Sepulchre Edith , Bours Vincent , Floch Aline , Pirenne France , Tshilumba Charles Kayembe , Batina‐Agasa Salomon 
Published: November 2022

AbstractObjectivesTo determine the prevalence of red blood cell (RBC) alloimmunisation and alloantibody specificity in sickle cell disease (SCD) patients in Kisangani, Democratic Republic of Congo (DRC) in comparison with those followed at the Centre Hospitalier Régional (CHR) de la Citadelle of Liège (Belgium).BackgroundData regarding RBC alloimmunisation (immune response of the organism to foreign erythrocyte antigens, antigens that lack on its own RBC) in SCD patients are scarce in sub‐Saharan Africa.MethodsWe conducted a multi‐site‐based cross‐sectional study among 125 SCD patients at Kisangani and 136 at the CHR de la Citadelle of Liège. The diagnosis of SCD was confirmed by high‐performance liquid chromatography. Alloantibodies were screened using the agglutination technique on gel cards and their specificity determined using 11 and/or 16 cell panels. Statistical analyses were carried out using SPSS.ResultsThe prevalence of RBC alloimmunisation was 9.6% among SCD patients in Kisangani versus 22.8% in those of Liège. At Kisangani as well as at Liège, the median age of alloimmunised patients was higher than that of non‐alloimmunised patients, 15.5 years (IQR:4.8–19.8) and 24 years (IQR:14–31) versus 10 years (IQR: 6.5–17) and 17 years (IQR:12–24), respectively. The median number of blood units was higher in both Kisangani and Liège immunised patients compared to non‐immunised patients, 8 (IQR:5–11) versus 5 (IQR:3–13) and 41(IQR:6–93) versus 6.5(3–37) respectively. At Kisangani (N = 14), the most frequent antibodies were anti‐D (28.6%) and anti‐C versus anti‐E (13.6%), anti‐S (13.6%) and anti‐Lea (11.4%) at Liège (N = 44).ConclusionsThese findings stated that alloimmunisation is a common complication in SCD patients in the DRC. In the resource‐limited setting of this country, blood transfusion with minimal ABO, D, C and E antigen matching in addition to the use of compatibility test could significantly reduce the incidence of this complication.
Response of invasive perennial western ragweed (Ambrosia psilostachya) to chemical and mechanical control
Authors: Saberi Hesan , Yousefi Ali Reza , Pouryousef Majid , Birbaneh Jafar Asghari , Tokasi Somayeh 
Published: November 2022

AbstractWestern ragweed (Ambrosia psilostachya) is an invasive species in spring‐sown crops that can also cause allergic rhinitis and asthma due to its allergenic pollen. In order to evaluate the chemical and mechanical control of western ragweed, two separate experiments were conducted in 2019 and 2020. Chemical treatments included non‐treated plots, the combination of glyphosate and 2,4‐D, glyphosate + ammonium sulfate, bentazon, imazethapyr + surfactant, picloram, 2,4‐D, and mechanical treatments included: once mowing at 3–4 leaf stage, once mowing before male flowers' emergence, once mowing early female flowering stage, two mowings at 3–4 leaf stage, two mowings before male flowers' emergence. Results of these studies indicated that in both years, picloram at 0.96 kg ai ha−1 and the combination of 2,4‐D and glyphosate at 1.23 + 0.72 kg ai ha−1 provided more than 90% control of western ragweed and reduced plant height, dry weight, and density. The application of imazethapyr and bentazon, respectively, at the rates of 0.1 and 0.96 kg ai ha−1 did not cause visual damage. The mowing shortly before flowering was the most effective mechanical treatment for western ragweed control. In order to the efficient management of the western ragweed, we suggest that the mowing treatments if appropriately timed and application of glyphosate plus 2, 4‐D at 1.23 + 0.72 kg ai ha−1, and picloram at 0.96 kg ai ha−1 can prevent western ragweed from spreading by suppressing growth and reducing seed production.
Upper Extremity Joint Torque Estimation Through an Electromyography-Driven Model
Authors: Tahmid Shadman , Font-Llagunes Josep M. , Yang James 
Published: November 2022

Abstract
Cerebrovascular accidents like a stroke can affect the lower limb as well as upper extremity joints (i.e., shoulder, elbow, or wrist) and hinder the ability to produce necessary torque for activities of daily living. In such cases, muscles’ ability to generate forces reduces, thus affecting the joint’s torque production. Understanding how muscles generate forces is a key element to injury detection. Researchers have developed several computational methods to obtain muscle forces and joint torques. Electromyography (EMG) driven modeling is one of the approaches to estimate muscle forces and obtain joint torques from muscle activity measurements. Musculoskeletal models and EMG-driven models require necessary muscle-specific parameters for the calculation. The focus of this study is to investigate the EMG-driven approach along with an upper extremity musculoskeletal model to determine muscle forces of two major muscle groups, biceps brachii and triceps brachii, consisting of seven muscle-tendon units. Estimated muscle forces are used to determine the elbow joint torque. Experimental EMG signals and motion capture data are collected for a healthy subject. The musculoskeletal model is scaled to match the geometric parameters of the subject. Then, the approach calculates muscle forces and joint moment for two tasks: simple elbow flexion extension and triceps kickback. Individual muscle forces and net joint torques for both tasks are estimated. The study also has compared the effect of muscle-tendon parameters (optimal fiber length and tendon slack length) on the estimated results.
Tubular Lockup Prediction in Deviated Wells Using Markov Chains
Authors: Ogundare Oluwatosin , Fagbemi Samuel 
Published: November 2022

Abstract
The effect of friction in well drilling operations is especially important in deviated wells and in cases where the impact of axial compressive loads on a drillstring decreases significantly with vertical depth and transversal displacement. The prevailing theories hope to determine the critical buckling loads analytically using the Paslay-Dawson equation with the hope of minimizing the event of tubular buckling in a principled way. In practice, there is very little that can be done to change the nature of a formation except to minimize the friction drag force by pumping friction reducers into the wellbore/borehole which consequently enhances the propagation of axial compressive forces. Determining the tubular lockup region accurately is possible with high fidelity and high-resolution friction profiling of the formation using models that determine critical buckling loads as a function of drag friction. Economically, it is important to determine ahead of time the friction factor or coefficient profile of a formation to establish if and where tubular lockup would occur, which consequently reduces drilling costs by pumping a friction reducer when it is needed and not before. The main idea of this paper is therefore to introduce a model that generates a high-resolution k-point friction profile for a formation using Markov chains. The model is then applied to predict the transition probabilities for friction drag in a reservoir with an accuracy of 86.8%.
Use of ultrasound biomicroscopy to predict the outcome of anterior segment reconstruction in congenital fibrovascular pupillary membrane with secondary glaucoma
Authors: Zhu Yingting , Fang Lei , Oatts Julius , Han Ying , Lin Shufen , Chen Liming , Liu Xing , Zhong Yimin 
Published: November 2022

AimsTo evaluate the efficacy and safety of anterior segment reconstruction (ASR) in congenital fibrovascular pupillary membrane-induced secondary glaucoma (CFPMSG) basing ultrasound biomicroscopy (UBM) classification.MethodsThis ambispective cohort study enrolled patients with CFPMSG who underwent ASR between January 2014 and September 2020. Comprehensive ophthalmic examinations and UBM were performed before surgery and postoperatively. The patients were classified into three types according to the UBM configurations. Anterior chamber recovery (ACR) was defined as deepening in anterior chamber (≥1.5 mm all through final follow-up (FFU), while success following ASR was defined as ACR and intraocular pressure (IOP)≤21 mm Hg.Results25 eyes of 25 patients underwent ASR (average age at operation 5.8±5.0 months, 48% girls) with FFU 15.8±16.9 months. Enrolled subjects were classified into type Ⅰ (11 eyes), type Ⅱ (11 eyes) and type Ⅲ (3 eyes). After ASR, 23 eyes (92%) achieved ACR, and the mean ACD increased in all groups (p=0.006, <0.001 and 0.003, respectively). Eyes with types Ⅰ and Ⅱ demonstrated a reduction of IOP (p=0.009 and 0.002, respectively). ASR success rate was highest in type Ⅰ (72.9%) compared with types Ⅱ and Ⅲ (18.2% and 0%, respectively; p=0.011). ASR led to decreased number of antiglaucoma medications for type Ⅰ CFPMSG at FFU (p=0.016). No vision-threatening postoperative complications occurred.ConclusionsASR for CFPMSG results in increased ACD and improvement in IOP. Postoperative IOP control was best in type Ⅰ CFPMSG but not as effective in types Ⅱ and Ⅲ. UBM-based classification helps to predict the surgical outcome of ASR in CFPMSG.
Effect of kangaroo mother care initiated in community settings on financial risk protection of low-income households: a randomised controlled trial in Haryana, India
Authors: Choudhary Tarun Shankar , Mazumder Sarmila , Haaland Oystein A , Taneja Sunita , Bahl Rajiv , Martines Jose , Bhan Maharaj Kishan , Norheim Ole Frithjof , Sommerfelt Halvor , Bhandari Nita , Johansson Kjell Arne 
Published: November 2022

IntroductionMany families in low-income and middle-income countries have high out-of-pocket expenditures (OOPE) for healthcare, and some face impoverishment. We aimed to assess the effect of Kangaroo Mother Care initiated in community setting (ciKMC) on financial risk protection estimated by healthcare OOPE, catastrophic healthcare expenditure (CHE) and impoverishment due to healthcare seeking for low birthweight infants, using a randomised controlled trial design.MethodsWe included 4475 low birthweight infants randomised to a ciKMC (2491 infants) and a control (1984 infants) arm, in a large trial conducted between 2017 and 2018 in Haryana, India. We used generalised linear models of the Gaussian family with an identity link to estimate the mean difference in healthcare OOPE, and Cox regression to estimate the HRs for CHE and impoverishment, between the trial arms.ResultsOverall, in the 8-week observation period, the mean healthcare OOPE per infant was lower (US$20.0) in the ciKMC arm compared with the control arm (US$25.6) that is, difference of −US$5.5, 95% CI −US$11.4 to US$0.3, p=0.06). Among infants who sought care it was US$8.5 (95% CI −US$17.0 to −US$0.03, p=0.03) lower in the ciKMC arm compared with the control arm. The HR for impoverishment due to healthcare seeking was 0.56 (95% CI 0.36 to 0.89, p=0.01) and it was 0.91 (95% CI 0.74 to 1.12, p=0.37) for CHE.ConclusionciKMC can substantially reduce the cost of care seeking and the risk of impoverishment for households. Our findings show that supporting mothers to provide KMC to low birthweight infants at home, in addition to reducing early infant mortality, may provide financial risk protection.Trial registration numberCTRI/2017/10/010114