bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploration of adulteration in common raw spices using antenna-based sensor
Authors: Nitika  , Kaur Jaswinder , Khanna Rajesh 
Published: November 2022

AbstractThe ability of food products to store and dissipate electromagnetic energy is determined by the material's dielectric properties. In relation to this phenomenon, a non-destructive technique is presented for food evaluation based on the shift in resonant frequency and reflection coefficient magnitude value of the proposed slot-loaded microstrip line-fed antenna-based sensor caused by the change in dielectric properties of the food material. In this work, a miniaturized antenna sensor of 10 × 10 mm2 size comprised of a dielectric substrate FR-4 with permittivity (εr) = 4.4 having ground plane at the bottom and a radiating element at the top is designed to operate at 13.3 GHz. Three samples of spices, i.e. red chilli powder, black pepper powder, and turmeric powder, are considered for quality monitoring whose relationship in terms of reflection coefficient, resonant frequency, and dielectric permittivity at 13.17, 12.61, and 13.09 GHz respectively is analyzed. Further, second-order polynomial model is derived to predict dielectric permittivity of the material under test with high accuracy. The experimental procedure of this proposed sensor is based upon the interaction of the sample food materials with the electromagnetic field owing to shift in resonant frequency as a function of dielectric permittivity of the samples. The proposed antenna sensor has a Q-factor of 409, showing significantly high sensitivity of 280 MHz with 98% accuracy and standard deviation less than the difference between unadulterated and adulterated values, giving resolution high enough to distinguish adulteration with an acceptable statistical accuracy.
The Southern Farmers’ Alliance, Populists, and lynching
Authors: Chamberlain Adam , Yanus Alixandra B. 
Published: November 2022

AbstractThe lynching literature often considers how the Populist Party affected lynching, yet the Southern Farmers’ Alliance—a short-lived but influential voluntary association that mobilized large numbers of white farmers—is overlooked. We argue that this is a critical oversight, as the Alliance was the origin of populism in the South. Specifically, we hypothesize that where the Alliance had more local organizations, the greater the likelihood of lynching from 1888 to 1895, the peak period of populism. To test this, we focus on two states with different experiences with the Alliance: North Carolina, in which the state’s Alliance was a strong supporter of the Populist Party, and South Carolina, where the Democrats sought to court Alliancemen and deter the creation of, and voting for, the Populist Party. Our empirical findings reveal that lynchings were more common in counties where the Farmers’ Alliance had more organizations in South Carolina, but no similar connection exists in North Carolina. These findings suggest that the Southern Farmers’ Alliance is, at times, pivotal to understanding populism’s connection to lynching in the late-nineteenth century American South.
The Multi‐Decadal Response to Net Zero CO2 Emissions and Implications for Emissions Policy
Authors: Jenkins Stuart , Sanderson Ben , Peters Glen , Frölicher Thomas L. , Friedlingstein Pierre , Allen Myles 
Published: November 2022

AbstractHow confident are we that CO2 emissions must reach net zero or below to halt CO2‐induced warming? The IPCC's sixth assessment report concluded that “limiting human‐induced global warming to a specific level requires … reaching at least net zero CO2 emissions.” This is much stronger language than the special report on the global warming of 1.5°C, which concluded that reaching net zero CO2 emissions would be sufficient. Here we show that “approximately net zero” is better supported than “at least net zero.” We estimate the rate of adjustment to zero emissions (RAZE) parameter (−0.24 to +0.17%/yr), defined as the fractional change in CO2‐induced warming after CO2 emissions cease. The RAZE determines the CO2 emissions compatible with halting warming over multiple decades: in 1.5°C‐consistent scenarios, CO2 emissions consistent with halting anthropogenic warming are +2.2 GtCO2/yr (5–95th percentile range spans −7.3 to +6.2 GtCO2/yr), similar to the expected emissions from unmodelled Earth system feedbacks.
High‐Resolution Fault‐Rupture Imaging by Combining a Backprojection Method With Binarized MUSIC Spectral Image Calculation
Authors: Oshima Mitsutaka , Takenaka Hiroshi , Matsubara Makoto 
Published: November 2022

AbstractBackprojection (BP) methods are widely used for estimating the fault rupture processes; however, they are inherently susceptible to noise. Hence, noise suppression is an important research target. In this paper, we develop a fault rupture imaging method by combining beamforming‐based BP and MUltiple Signal Classification (MUSIC), which realizes artificial noise suppression at a high spatial resolution. The stations are grouped into arrays according to the SH wave radiation coefficients, and MUSIC analysis is performed on each array. The MUSIC spectral images of these arrays are binarized and then multiplied by the BP images. Spatial filtering is also applied to the images based on the possible range of the rupture velocity and rise time. When tested using synthetic test data, the proposed method worked as expected. We then applied this method to the 2016 Kumamoto earthquake by interpolating the travel times from the observed travel times of relocated hypocenters using a 3‐D velocity structure model. In the area of large slip and slip rate approximately 30–50 km from the hypocenter on the Futagawa fault, the spatiotemporal evolution of the fault ruptures and waveform inversion results were generally in harmony. The distributions of the low‐ and high‐frequency seismic radiations are complementary, as is understood in the context of fault rupture physics. This method can aid in understanding and modeling the details of seismic radiation sources, enabling the accurate prediction of strong ground motion even in near‐fault areas.
Crustal Seismic Anisotropy Along the Continental Margin in Western Canada From Receiver Function Analysis
Authors: Tracey Kyryliuk T. , Audet P. , Gosselin J. M. , Schaeffer A. J. 
Published: November 2022

AbstractCoastal British Columbia (BC), Canada, has the highest seismic hazard in the country due to convergent and transpressive deformation at offshore plate boundaries between the Pacific, Juan de Fuca (JdF) and North American (NA) plates. Further landward, the crust of the NA plate is made up of several geologically unique exotic terranes and is unusually thin. Investigating the geophysical features in this area can help us better constrain its tectonic history and the geophysical processes that are currently underway. Here, we conduct an analysis of teleseismic body‐wave scattering data (i.e., receiver functions [RFs]) recorded at stations across western coastal BC including northern Vancouver Island and southeastern Alaska. Using these RFs, we perform a harmonic decomposition with respect to earthquake back‐azimuths to determine the orientation of seismic anisotropy over a series of depth ranges, attributable to either mineral alignment or dipping structures. We find a coherent pattern of margin‐parallel orientations at upper crustal depths that persist onto the mainland at distances 420 km from the margin. Furthermore, dominant receiver function orientations at depth are attributed to dipping faults and interfaces, and fabrics due to lower crustal shearing or inherited from tectonic assembly along the margin. This work supports models for the tectonic assembly of this region that involve a combination of plate subduction and transpressive motion along crustal scale faults that pervade a wide portion of the margin. This work also helps to constrain the current geometries of the subducting Pacific and JdF slabs.
Aeolian Erosion Thresholds for Cohesive Sand
Authors: Besnard J.‐B. , Dupont P. , El Moctar A. Ould , Valance A. 
Published: November 2022

AbstractMoisture is known to affect sand cohesion and therefore transport threshold and rate of aeolian sand transport. Aeolian sand transport has been widely documented for dry sand beds, but much more sparsely in the context of moist sand beds. One major challenge in the collection of reliable field or laboratory data for moist sand is to have an accurate control of the moisture level within the sand bed, which is prone to strongly vary over time and space due to evaporation. To suppress the variability of moisture content due to evaporation, we devised a new approach based on the use of a non‐volatile liquid (namely silicon oil instead of water) which ensures a proper control of the liquid content. We thus conducted wind‐tunnel experiments employing this approach and observed that the aerodynamic threshold friction velocity increases linearly with liquid content, while the dynamic threshold is found to be unchanged in the range of cohesion strength investigated in this study. These outcomes indicate that the difference between the static and dynamic threshold increases with increasing cohesion. This may have strong implications on aeolian transport of moist sand, and in particular on hysteretic behaviors.
Tulp1 deficiency causes early-onset retinal degeneration through affecting ciliogenesis and activating ferroptosis in zebrafish
Authors: Jia Danna , Gao Pan , Lv Yuexia , Huang Yuwen , Reilly James , Sun Kui , Han Yunqiao , Hu Hualei , Chen Xiang , Zhang Zuxiao , Li Pei , Luo Jiong , Shu Xinhua , Tang Zhaohui , Liu Fei , Liu Mugen , Ren Xiang 
Published: November 2022

AbstractMutations in TUB-like protein 1 (TULP1) are associated with severe early-onset retinal degeneration in humans. However, the pathogenesis remains largely unknown. There are two homologous genes of TULP1 in zebrafish, namely tulp1a and tulp1b. Here, we generated the single knockout (tulp1a−/− and tulp1b−/−) and double knockout (tulp1-dKO) models in zebrafish. Knockout of tulp1a resulted in the mislocalization of UV cone opsins and the degeneration of UV cones specifically, while knockout of tulp1b resulted in mislocalization of rod opsins and rod-cone degeneration. In the tulp1-dKO zebrafish, mislocalization of opsins was present in all types of photoreceptors, and severe degeneration was observed at a very early age, mimicking the clinical manifestations of TULP1 patients. Photoreceptor cilium length was significantly reduced in the tulp1-dKO retinas. RNA-seq analysis showed that the expression of tektin2 (tekt2), a ciliary and flagellar microtubule structural component, was downregulated in the tulp1-dKO zebrafish. Dual-luciferase reporter assay suggested that Tulp1a and Tulp1b transcriptionally activate the promoter of tekt2. In addition, ferroptosis might be activated in the tulp1-dKO zebrafish, as suggested by the up-regulation of genes related to the ferroptosis pathway, the shrinkage of mitochondria, reduction or disappearance of mitochondria cristae, and the iron and lipid droplet deposition in the retina of tulp1-dKO zebrafish. In conclusion, our study establishes an appropriate zebrafish model for TULP1-associated retinal degeneration and proposes that loss of TULP1 causes defects in cilia structure and opsin trafficking through the downregulation of tekt2, which further increases the death of photoreceptors via ferroptosis. These findings offer insight into the pathogenesis and clinical treatment of early-onset retinal degeneration.
Stereoisomeric engineering of aggregation-induced emission photosensitizers towards fungal killing
Authors: Zhu Wenping , Li Ying , Guo Shaoxun , Guo Wu-Jie , Peng Tuokai , Li Hui , Liu Bin , Peng Hui-Qing , Tang Ben Zhong 
Published: November 2022

AbstractFungal infection poses and increased risk to human health. Photodynamic therapy (PDT) as an alternative antifungal approach garners much interest due to its minimal side effects and negligible antifungal drug resistance. Herein, we develop stereoisomeric photosensitizers ((Z)- and (E)-TPE-EPy) by harnessing different spatial configurations of one molecule. They possess aggregation-induced emission characteristics and ROS,viz.1O2and O2−•generation capabilities that enable image-guided PDT. Also, the cationization of the photosensitizers realizes the targeting of fungal mitochondria for antifungal PDT killing. Particularly, stereoisomeric engineering assisted by supramolecular assembly leads to enhanced fluorescence intensity and ROS generation efficiency of the stereoisomers due to the excited state energy flow from nonradiative decay to the fluorescence pathway and intersystem (ISC) process. As a result, the supramolecular assemblies based on (Z)- and (E)-TPE-EPy show dramatically lowered dark toxicity without sacrificing their significant phototoxicity in the photodynamic antifungal experiments. This study is a demonstration of stereoisomeric engineering of aggregation-induced emission photosensitizers based on (Z)- and (E)-configurations.
The evolution of reproductive modes and life cycles in amphibians
Authors: Liedtke H. Christoph , Wiens John J. , Gomez-Mestre Ivan 
Published: November 2022

AbstractAmphibians have undergone important evolutionary transitions in reproductive modes and life-cycles. We compare large-scale macroevolutionary patterns in these transitions across the three major amphibian clades: frogs, salamanders, and caecilians. We analyse matching reproductive and phylogenetic data for 4025 species. We find that having aquatic larvae is ancestral for all three groups and is retained by many extant species (33–44%). The most frequent transitions in each group are to relatively uncommon states: live-bearing in caecilians, paedomorphosis in salamanders, and semi-terrestriality in frogs. All three groups show transitions to more terrestrial reproductive modes, but only in caecilians have these evolved sequentially from most-to-least aquatic. Diversification rates are largely independent of reproductive modes. However, in salamanders direct development accelerates diversification whereas paedomorphosis decreases it. Overall, we find a widespread retention of ancestral modes, decoupling of trait transition rates from patterns of species richness, and the general independence of reproductive modes and diversification.
Patterned mechanical feedback establishes a global myosin gradient
Authors: Gustafson Hannah J. , Claussen Nikolas , De Renzis Stefano , Streichan Sebastian J. 
Published: November 2022

AbstractMorphogenesis, the coordinated execution of developmental programs that shape embryos, raises many fundamental questions at the interface between physics and biology. In particular, how the dynamics of active cytoskeletal processes are coordinated across the surface of entire embryos to generate global cell flows is poorly understood. Two distinct regulatory principles have been identified: genetic programs and dynamic response to mechanical stimuli. Despite progress, disentangling these two contributions remains challenging. Here, we combinein totolight sheet microscopy with genetic and optogenetic perturbations of tissue mechanics to examine theoretically predicted dynamic recruitment of non-muscle myosin II to cell junctions duringDrosophilaembryogenesis. We find dynamic recruitment has a long-range impact on global myosin configuration, and the rate of junction deformation sets the rate of myosin recruitment. Mathematical modeling and high frequency analysis reveal myosin fluctuations on junctions around a mean value set by mechanical feedback. Our model accounts for the early establishment of the global myosin pattern at 80% fidelity. Taken together our results indicate spatially modulated mechanical feedback as a key regulatory input in the establishment of long-range gradients of cytoskeletal configurations and global tissue flow patterns.
Cooperative binding of T cell receptor and CD4 to peptide-MHC enhances antigen sensitivity
Authors: Rushdi Muaz Nik , Pan Victor , Li Kaitao , Choi Hyun-Kyu , Travaglino Stefano , Hong Jinsung , Griffitts Fletcher , Agnihotri Pragati , Mariuzza Roy A. , Ke Yonggang , Zhu Cheng 
Published: November 2022

AbstractAntigen recognition by the T cell receptor (TCR) of CD4+ T cells can be greatly enhanced by the coreceptor CD4. Yet, understanding of the molecular mechanism is hindered by the ultra-low affinity of CD4 binding to class-II peptide-major histocompatibility complexes (pMHC). Here we show, using two-dimensional (2D) mechanical-based assays, that the affinity of CD4–pMHC interaction is 3-4 logs lower than that of cognate TCR–pMHC interactions, and it is more susceptible to increased dissociation by forces (slip bond). In contrast, CD4 binds TCR-pre-bound pMHC at 3-6 logs higher affinity, forming TCR–pMHC–CD4 tri-molecular bonds that are prolonged by force (catch bond), and modulated by protein mobility on the cell membrane, indicating profound TCR-CD4 cooperativity. Consistent with a tri-crystal structure, using DNA origami as a molecular ruler to titrate spacing between TCR and CD4 we show that 7-nm proximity optimizes TCR–pMHC–CD4 tri-molecular bond formation with pMHC. Our results thus provide deep mechanistic insight into CD4 enhancement of TCR antigen recognition.
Feedforward growth rate control mitigates gene activation burden
Authors: Barajas Carlos , Huang Hsin-Ho , Gibson Jesse , Sandoval Luis , Del Vecchio Domitilla 
Published: November 2022

AbstractHeterologous gene activation causes non-physiological burden on cellular resources that cells are unable to adjust to. Here, we introduce a feedforward controller that actuates growth rate upon activation of a gene of interest (GOI) to compensate for such a burden. The controller achieves this by activating a modified SpoT enzyme (SpoTH) with sole hydrolysis activity, which lowers ppGpp level and thus increases growth rate. An inducible RelA+ expression cassette further allows to precisely set the basal level of ppGpp, and thus nominal growth rate, in any bacterial strain. Without the controller, activation of the GOI decreased growth rate by more than 50%. With the controller, we could activate the GOI to the same level without growth rate defect. A cell strain armed with the controller in co-culture enabled persistent population-level activation of a GOI, which could not be achieved by a strain devoid of the controller. The feedforward controller is a tunable, modular, and portable tool that allows dynamic gene activation without growth rate defects for bacterial synthetic biology applications.
Azide-functionalized ligand enabling organic–inorganic hybrid dielectric for high-performance solution-processed oxide transistors
Authors: Lee Juhyeok , Hassan Syed Zahid , Lee Sangjun , Sim Hye Ryun , Chung Dae Sung 
Published: November 2022

AbstractWe propose a highly efficient crosslinking strategy for organic–inorganic hybrid dielectric layers using azide-functionalized acetylacetonate, which covalently connect inorganic particles to polymers, enabling highly efficient inter- and intra-crosslinking of organic and inorganic inclusions, resulting in a dense and defect-free thin-film morphology. From the optimized processing conditions, we obtained an excellent dielectric strength of over 4.0 MV cm−1, a high dielectric constant of ~14, and a low surface energy of 38 mN m−1. We demonstrated the fabrication of exceptionally high-performance, hysteresis-free n-type solution-processed oxide transistors comprising an In2O3/ZnO double layer as an active channel with an electron mobility of over 50 cm2 V−1 s−1, on/off ratio of ~107, subthreshold swing of 108 mV dec−1, and high bias-stress stability. From temperature-dependent I–V analyses combined with charge transport mechanism analyses, we demonstrated that the proposed hybrid dielectric layer provides percolation-limited charge transport for the In2O3/ZnO double layer under field-effect conditions.
New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance
Authors: Klein-Rodewald Tanja , Micklich Kateryna , Sanz-Moreno Adrián , Tost Monica , Calzada-Wack Julia , Adler Thure , Klaften Matthias , Sabrautzki Sibylle , Aigner Bernhard , Kraiger Markus , Gailus-Durner Valerie , Fuchs Helmut ,   German Mouse Clinic Consortium, Aguilar Pimentel Juan Antonio , Becker Lore , Garrett Lillian , Hölter Sabine M. , Prehn Cornelia , Rácz Ildikó , Rozman Jan , Puk Oliver , Schrewe Anja , Schulz Holger , Adamski Jerzy , Busch Dirk H. , Esposito Irene , Wurst Wolfgang , Stoeger Claudia , Gründer Albert , Pahl Heike , Wolf Eckhard , Hrabe de Angelis Martin , Rathkolb Birgit 
Published: November 2022

AbstractGastro-intestinal stromal tumors and acute myeloid leukemia induced by activating stem cell factor receptor tyrosine kinase (KIT) mutations are highly malignant. Less clear is the role of KIT mutations in the context of breast cancer. Treatment success of KIT-induced cancers is still unsatisfactory because of primary or secondary resistance to therapy. Mouse models offer essential platforms for studies on molecular disease mechanisms in basic cancer research. In the course of the Munich N-ethyl-N-nitrosourea (ENU) mutagenesis program a mouse line with inherited polycythemia was established. It carries a base-pair exchange in the Kit gene leading to an amino acid exchange at position 824 in the activation loop of KIT. This KIT variant corresponds to the N822K mutation found in human cancers, which is associated with imatinib-resistance. C3H KitN824K/WT mice develop hyperplasia of interstitial cells of Cajal and retention of ingesta in the cecum. In contrast to previous Kit-mutant models, we observe a benign course of gastrointestinal pathology associated with prolonged survival. Female mutants develop mammary carcinomas at late onset and subsequent lung metastasis. The disease model complements existing oncology research platforms. It allows for addressing the role of KIT mutations in breast cancer and identifying genetic and environmental modifiers of disease progression.
Analysis on the temporal and spatial characteristics of the shallow soil temperature of the Qinghai-Tibet Plateau
Authors: Li Yujie , Zhang Cunjie , Li Zhenchao , Yang Liwei , Jin Xiao , Gao Xiaoqing 
Published: November 2022

AbstractShallow soil refers to the soil layer within the 50 cm depth. Shallow soil temperature (ST) directly or indirectly affects many processes in the soil, such as seed germination, plant growth, and water evaporation. Therefore, the study of shallow ST is of great significance in understanding the surface energy, water cycle, ecology and climate change. This work collected observational data from 141 meteorological stations on the Qinghai-Tibet Plateau from 1981 to 2020 and ERA5 reanalysis data, used the “Moving Surface Spline Interpolation Algorithm Based on Green’s Function” and “Fuzzy C-means algorithm”, and analyzed the temporal and spatial change characteristics of ST at different levels. The results showed that 1) the temperature increase of 0–20 cm (the surface layer of the shallow soil) was roughly the same. The average annual ST was 9.15–9.57°, and the interdecadal variabilities were 0.49–0.53 K/10a. The average annual ST of 40 cm (the bottom layer) was 8.69°, and the interdecadal variability reached 0.98 K/10a. 2) Considering the 7 regions, the warming trend was obvious, and there were certain regional differences. The average annual ST in different regions ranged from 5.2 (northeastern Plateau) to 17.1 °C (western Sichuan Plateau), with a difference of nearly 12 K. The standard deviation ranged from 0.40 (western Sichuan Plateau) to 0.61 K (Qiangtang Plateau), with a difference of 0.21 K. 3) The errors of the obtained grid data were basically less than 3%, which were much smaller than the errors obtained from the ERA5 reanalysis data. This work is significant for understanding the characteristics of ST evolution and land‒atmosphere interactions on the Qinghai-Tibet Plateau and provides important data support for improving the underlying surface boundary conditions of models.