bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality of Life of Patients After Colorectal Cancer Surgery in Soba University Hospital, Sudan: A Cross-Sectional Study
Authors: Adam Mohammed A. , Salih Alaa A. , Koko Abubaker E.A , Altalib Sami , MohamedAhmed Ali Yasen Yasen , Hasabo Elfatih A. , Ahmed Ghassan E. , Maroof Mohammed Khalid Abdalla , Salim Omer El Faroug H 
Published: January 2022

Abstract
Background: Colorectal surgery is reported to have significant effects on patients, both physically and psychologically. On other hand, infections are found to be a major risk factor in Sudan and Sub-Saharan Africa such as intestinal Schistosoma colitis, especially those presenting with sigmoid colonic adenocarcinoma.Aim of the study: To assess the quality of life of patients after colorectal cancer surgery and the effect of the stoma on their life.Methods: A descriptive cross-sectional hospital-based study was done at Soba University Hospital. A sample of 72 patients with colorectal cancer who had undergone colorectal surgery was fully covered and interviewed using the SF-36 Quality of life standard questionnaire.Results: The total sample was 72 with a mean age of 51.1 ± 14.6 years. 79% were married, 70% were working, with free business being the most encountered occupation (36.1%). However, only 48.6% were still employed at the time of surgery. Regarding the mental health component, there was a significant difference in social functioning domain mean scores between patients who were employed and unemployed patients. Where the physical functioning and role physical domains were found to differ significantly with the different educational attainment of patients. Patients who did not undergo radiation therapy reported higher mean scores of role limitation due to physical problems, compared to patients who received radiation therapy.Conclusion: Our result found the quality of life was affected negatively in terms of the level of pain and presence of colostomy with sexual activity affection but the other parameters were not strongly affected.
RNA-Seq Coupling Two Different Methods of Castration Reveals New Insights Into Androgen Deficiency-caused Degereration of Submaxillary Gland in Male Sprague Dawley Rats
Authors: Han Xingfa , Xia Xu , Zhuo Yong , Hua Lun , Yu Guozhi , Bu Guixian , Cao Xiaohan , Du XiaoGang , Liang Qiuxia , Zeng Xianyin , Meng Fengyan 
Published: January 2022

Abstract
Backgroud: Salivary gland degeneration and dysfunction are common symptoms that occur after sex hormone deprivation, but the underlying mechanisms remain largely unknown. Additionally, immunocastration, which causes drop of sex hormones, has been developed as an alternative to surgical castration, however whether it exerts similar effects as surgical castration on the salivary glands is unknown. Through histological and RNA-seq analysis, we assessed changes in morphology and transcriptome of submaxillary gland (SMG) in response to immunocastration (IM) versus surgical castration (bilateral orchiectomy, ORC). Results: Compared to intact males (EM), ORC caused a dramatical degeneration of SMG in rats, as evidenced by both decreased (P < 0.01) SMG weight and organ index, and by decreased (P < 0.01) quantity of SMG acini and ducts. IM had minimal effects (P > 0.05) on SMG weight and organ index, but it still caused degeneration (P < 0.05) of the acini and ducts. Even though, the quantity of both SMG acini and ducts was much higher (P < 0.001) in IM than in ORC. Functional enrichment analysis of the common regulated genes by ORC/IM revealed disrupted epithelial cell development, angiogenesis, anatomical structure morphogenesis and enhanced cell death are associated with SMG degeneration in deprivation of androgens. Integrated data analysis shown that there existed a selective hyperfunction of SMG ribosome and mitochondrion in ORC but not in IM, which might be associated with more severe degeneration of SMG in ORC than in IM. Conclusions: Our findings suggested that both surgical castration and immunocastration caused SMG degeneration by disrupting epithelial cell development, angiogenesis, anatomical structure morphogenesis and enhancing cell death. But, surgical castration selectively induced hyperfunction of SMG ribosome and mitochondrion, thus causing more severe degeneration of SMG than immunocastration.
Complementary Omics Strategies to Dissect p53 Signaling Networks Under Nutrient Stress
Authors: Galhuber Markus , Michenthaler Helene , Heininger Christoph , Reinisch Isabel , Nössing Christoph , Krstic Jelena , Kupper Nadja , Moyschewitz Elisabeth , Auer Martina , Heitzer Ellen , Ulz Peter , Birner-Gruenberger Ruth , Liesinger Laura , Lenihan-Geels Georgia Ngawai , Oster Moritz , Spreitzer Emil , Chiozzi Riccardo Zenezini , Schulz Tim J. , Schupp Michael , Madl Tobias , J.R. Albert , Prokesch Andreas 
Published: January 2022

Abstract
Signaling trough p53 is a major cellular stress response mechanism and increases upon nutrient stresses such as starvation. Here, we show in a human hepatoma cell line that starvation leads to robust nuclear p53 stabilization. Using BioID, we determine the cytoplasmic p53 interaction network within the immediate-early starvation response and show that p53 is dissociated from several metabolic enzymes and the kinase PAK2 for which direct binding with the p53 DNA-binding domain was confirmed with NMR studies. Furthermore, proteomics after p53 immunoprecipitation (RIME) uncovered the nuclear interactome under prolonged starvation, where we confirmed the novel p53 interactors SORBS1 (insulin receptor signaling) and UGP2 (glycogen synthesis). Finally, transcriptomics after p53 re-expression revealed a distinct starvation-specific transcriptome response and suggested previously unknown nutrient-dependent p53 target genes. Together, our complementary approaches delineate several nodes of the p53 signaling cascade upon starvation, shedding new light on the mechanisms of p53 as nutrient stress sensor. Given the central role of p53 in cancer biology and the beneficial effects of fasting in cancer treatment, the identified interaction partners and networks could pinpoint novel pharmacologic targets to fine-tune p53 activity.
Chronic High-frequency Stimulation of the Subthalamic Nucleus Induces a Sustained Inhibition of Serotonergic System via Loss of Cell Phenotype
Authors: Alosaimi Faisal , Temel Yasin , Hescham Sarah , Witzig Victoria , Almasabi Faris , Tan Sonny , Jahanshahi Ali 
Published: January 2022

Abstract
Deep brain stimulation (DBS) of the subthalamic nucleus (STN) has become a standard treatment in Parkinson’s disease (PD). However, in a considerable number of patients debilitating psychiatric side-effects occur. Recent research has revealed that external stimuli can alter the neurotransmitters’ homeostasis in neurons, which is known as “neurotransmitter respecification”. Herein, we addressed if neurotransmitter respecification could be a mechanism by which DBS suppresses the serotonergic function in the dorsal raphe nucleus (DRN) leading to mood changes. We infused transgenic 5-HT-Cre (ePet-cre) mice with AAV viruses to achieve targeted expression of eYFP and the genetically encoded calcium indicator GCaMP6s in the DRN prior to methyl-4phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment. Mice received bilateral DBS electrodes in the STN and an optic fiber in the DRN for Ca2+ photometry. MPTP treated mice demonstrated behavioral and histological PD phenotype, whereas all STN-DBS animals exhibited an increased immobility time in the forced swim test, reduced Ca2+ activity, and loss of TPH2 expression in the DRN. Given the prominent role of Ca2+ transients in mediating neurotransmitter respecification, these results suggest a chronic loss of serotonergic phenotype in the DRN following STN-DBS. These findings indicate that loss of 5-HT cell phenotype may underlie the unwanted depressive symptoms following STN-DBS.
Candidate Gene and MicroRNA Biomarkers of Metastatic Renal Cell Carcinoma (MRCC) Response to Sunitinib
Authors: yang qiwei , yang wei , tian yijun , xu da , chu chuanmin , pan xiuwu , ye jianqing , chu jian , gan sishun , cao jianwei 
Published: January 2022

Abstract
Backgrounds: The incidence of renal cancer is relatively insidious, and some patients have been metastatic renal cancer at the initial visit. Sunitinib is the first-line systemic therapy for patients with metastatic renal cell carcinoma, however, there is scant analysis of its effect on genes and microRNAs.Methods: In this study, 8 differentially expressed microRNAs and 112 differentially expressed genes were designated by analyzing mRNA and microRNA data sets and weighted correlation network analysis (WGCNA).Results: NIPSNAP1 gene showed the most co-expression with other genes. Through the intersection of the microRNA target gene with our differentially expressed genes, we got 26 genes. KEGG and GO analysis showed that these genes were predominantly concentrated in Pathways in cancer, Sphingolipid metabolism and Glycosaminoglycan degradation. After we set the 26 genes and gene of WGCNA do intersection, received six genes, respectively is NIPSNAP1, SDC4, TBC1D9, NEU1, STK40 and PLAUR. Conclusion: Through subsequent cell, molecular and flow cytometry experiments, we found the PLAUR would play a crucial role in renal cell carcinoma (RCC) resistant to sunitinib, which will be available for new ideas to forecast sunitinib resistance and reverse sunitinib resistance.
Pasture Height of A Tropical Erect Grass Affecting Young Lamb Herbage Intake And Performance
Authors: Irigoyen Lívia Raymundo , Poli Cesar Henrique Espírito Candal , Corrêa Gladis Ferreira , Tontini Jalise Fabíola , López Ignacio Fernando , Silva Joseane Anjos da 
Published: January 2022

Abstract
Tropical erect grass pastures have high forage production potential in subtropical and tropical regions of the world. However, in this kind of pasture, the body weight gain of weaned lambs is usually below of their potential. We determined the effect of pasture height of an erect tropical grass, consequently its structure, on intake and performance of young lambs. The experiment was repeated in two years. Thirty young weaned lambs (4-5 months) were assigned, each year, to three Panicum maximum pasture heights: 1) Tall-75 cm; 2) Medium-50 cm and 3) Short-25 cm. Herbage mass, pasture height, plant morphological composition, pasture nutritional quality, lamb’s average daily gain, gain per hectare and herbage intake were measured. The experiment was installed in a completely randomized design, evaluated in two periods each year. The Short treatment presented, on average and for longer, the highest leaf:stem ratio (1.3 ± 0.23) and average daily gain (91 ± 10 g/day) in relation to the Medium and Tall treatments (68 ± 10 and 40 ± 13 g, respectively). There was a significant interaction between treatment*period for herbage intake. The Tall treatment showed a marked decrease in intake from the first to the second period. The pasture height management interferes in the speed at which the number of stems increases of a tropical erect grass, in relation to leaves. For a better performance of weaned young lambs during summer-autumn period, it is important to manage tropical erect grass pastures at lower height than is generally recommended, lower than 25 cm.
Green Synthesis of Different Nanoparticles and its Effect on Irrigation Water and Soil Properties and Origanum Majorana Productivity
Authors: Eissa Doaa , Hegab Rehab , Shady Ahmed Abou , kotp yousra 
Published: January 2022

Abstract
The term of nanotechnology has emerged recently in several fields of interest that refers to the researches and innovations that are concerned with making materials on a very small size close to the scale of atoms and molecules. In the present work, the effects of green synthesis of different nanoparticles on the quality of irrigation water, the availability of some heavy metals content in soil and the plant, and the productivity of Marjoram were studied in detail. The obtained results showed that the addition of nanoparticles (NPs) materials has resulted in noticeable variations in the removal percentages of Cu and Fe from aqueous solution. The maximum values obtained for adsorption of Cu (II) on ZnO, MgO, and SiO2 NPs, within pH (3–5) were 89.9%, 83.3%, and 68.36%, respectively. Whereas, the maximum adsorption values of Fe (III) at pH 3.3 were 82%, 80%, and 65% for ZnO, MgO, and SiO2 NPs, respectively. It was clearly seen that the effective of NPs application on reduction of the available Cu in the studied soil samples. The order of sequence for the effects of NPs application was found to take the following order Zn2 > Zn1 > Mg2 > Si2 > Mg 1 > Si1 > C (control). The highest values of the available Cu were observed in the control treatment, whereas the lowest values were obtained when Zn2 was added, and the same tendency was observed with the substantial concentrations of Fe. The addition of NPs to the soil samples had positively affected the Cu uptake via plant. The effects of NPs and the additions of Cu and Fe on the availability of NPK in the soil system were very completed and osculated from one treatment to another. The same tendency was observed with the total concentration of NPK in the plant.
Risk Factors of Dermatophytosis in Young Adults: A Nationwide Population-Based Study
Authors: Son Joon Ho , Doh Jee Yun , Han Kyungdo , Kim Yeong Ho , Han Ju Hee , Bang Chul Hwan , Park Young Min , Lee Ji Hyun 
Published: January 2022

Abstract
Dermatophytosis includes all fungal infections caused by dermatophytes in humans. Some risk factors for the development of subtypes of dermatophytosis have been studied; however, large-scale epidemiologic studies on risk factors for total dermatophytosis are scarce. We investigated the risk factors of dermatophytosis using a nationwide study. Total 4,532,655 subjects with dermatophytosis aged between 20 to 40 years were examined using data from the Korean National Health Insurance Service from 2009 to 2018. Women showed a lower risk of development of dermatophytosis compared to men (hazard ratio [HR], 0.848; 95% confidence interval [CI], 0.843–0.853). Subjects with elevated waist circumference (HR, 1.057; 95% CI, 1.048–1.065), heavy drinking (HR, 1.053; 95% CI, 1044–1.061), engaging in mild-to-heavy exercise (HR, 1.071; 95% CI, 1.064–1.077) had a higher risk of dermatophytosis. In addition, subjects with body mass index (BMI) of more than 30 kg/m2 exhibited a higher risk of dermatophytosis (HR, 1.36; 95% CI, 1.342–1.378) compared to those with BMIs in the range of 18.5 to 23 kg/m2. In this study, the risk of developing dermatophytosis significantly increased in individuals with elevated waist circumference or high BMI. Lifestyle modifications, including weight management, are suggested to be important in preventing dermatophytosis.
Traveling 10-day Waves at Mid-latitudes in the Troposphere and Lower Stratosphere Revealed by Radiosonde Observations and MERRA-2 Data in 2020
Authors: Huang Chunming , Yang Lingyun , Zhang Shaodong , Huang Kaiming , Gong Yun , Ma Zheng , Zhang Jian 
Published: January 2022

Abstract
Although the characteristics of the traveling 10-day waves (10DWs) above the middle stratosphere have been well explored, little research has been performed on the counterpart in the troposphere and lower stratosphere (TLS). In the present study, we use radiosonde observations and MERRA-2 data in 2020 to characterize traveling 10DWs in mid-latitudes in the TLS. Single-site observations in both hemispheres show that strong 10DW activities are always accompanied by strong eastward jets (10-13 km). MERRA-2 data indicates that in the troposphere the eastward-propagating modes with larger wavenumbers, i.e., E3, E4, E5 and E6 are dominant. While in the lower stratosphere the eastward- and westward-propagating modes with small zonal wavenumbers e.g., 1 and 2, are dominant. Further research on E3, E4, E5 and E6 modes in the troposphere of both hemispheres shows that all the wave activities are positively correlated to the background zonal wind. The refractive index squared reveal that a strong eastward jet is suitable for these four modes to propagate. However, just above the jet, the eastward wind decreases with altitude, and a thick evanescence region emerges above 15 km. E3, E4, E5 and E6 10DWs cannot propagate upward across the tropopause; as such this can explain why these four modes are weak or even indiscernible in the stratosphere and above. In the troposphere, E5 10DW at 32°S is the most dominant mode in 2020. A case study of the anomalously strong E5 10DW activity on May 12, 2020 indicates that the wave amplification resulted from the upward and equatorward transmission of wave energy flows. Moreover, the tropopause and equatorial region can prevent the propagations of wave energy flows of E5 10DW.
Trajectories of chemosensory and cognitive functions up to 1.5 years after acute COVID-19
Authors: Chang Xiaoyue , Chen Kepu , Ye Yuting , Gu Fei , Wu Yuli , Yuan Kaiqi , Wang Yanqing , Zhou Bin , Zhou Wen 
Published: January 2022

Abstract
There is an increasing recognition of neurological manifestations from SARS-CoV-2 infection. Quantifications of such manifestations and their long-term dynamics in the general infected population are of essence in understanding the health and socioeconomic burden of neurological complications of COVID-19. Through rigorous empirical testing of over 800 volunteers, we present here repeated cross-sectional and longitudinal data that depict the trajectories of chemosensory functions, cognitive performances and depressive symptoms up to 1.5 years after acute COVID-19 in discharged patients with respect to non-infected controls. Overall, deficits in smell, taste and chemesthesis slowly resolved within about a year of discharge. Concerningly, cognitive impairments –– independent of elevated depressive symptoms and evident even in those with nonsevere disease –– showed no sign of improvement over time. In people over 50 years, COVID-19 was associated with a substantially increased risk for mild cognitive impairment. Our findings urge for cognitive and emotional interventions targeting COVID-19 convalescents.
A Novel Gene, Le-Dd 10, Is Involved in Fruiting Body Formation of Lentinula Edodes
Authors: Kishikawa Akihiro , Hamada Satoshi , Kamei Ichiro , Fujimoto Yosuke , Miyazaki Kazuhiro , Yoshida Motonobu 
Published: January 2022

Abstract
The cDNA library prepared from Lentinula edodes, Hokken 600 (H600), primordia was screened by using cDNA expressed specifically in Dictyostelium discoideum prestalk as a probe. Twenty-one clones, Le-Dd 1~21, were isolated from the L. edodes primordia cDNA library. Functional analysis of each gene was carried out by transformation into protoplast cells from L. edodes Mori 252 (M252) mycelia with the overexpression vector pLG-RasF1 of each gene because M252 protoplast cells were transformed with 11-fold higher efficiency than H600 cells. Transformants with the overexpression vector of Le-Dd10 formed a fruiting body at almost the same time as H600, a positive control, although M252, a negative control, did not form a fruiting body under culture conditions. This suggested that Le-Dd10 is involved in the formation of fruiting bodies. Single-strand conformation polymorphism analysis revealed that Le-Dd10 is located on No. 4 linkage group of L. edodes. The properties of Le-Dd10 products were investigated by Western blotting analysis using polyclonal antibodies against GST:Le-Dd10 fusion proteins. As a result, 56-kDa, 27-kDa, and 14-kDa protein bands appeared in primordial and fruiting body stages, although the expected molecular weight of the Le-Dd10 product was 50 kDa.
ENO3 promotes colorectal cancer progression by enhancing cell glycolysis
Authors: Chen Jingyu , Zhang Zizhen , Ni Jiaojiao , Sun Jiawei , Ju Fangyu , Wang Zhuo , Wang Liangjing , Xue Meng 
Published: January 2022

Abstract
Background

Colorectal cancer (CRC) is among the leading cause of cancer-related morbidity and mortality worldwide. Aerobic glycolysis, as a metabolic hallmark of cancer, plays an important role in CRC progression. Enolase 3 (ENO3) is a glycolytic enzyme that catalyzes 2-phosphoglycerate into phosphoenolpyruvate, while its role in CRC is still unknown.
Methods

Bioinformatics analysis was performed to examine the expression changes and roles of ENO3 in CRC patients from public databases. Then, ENO3 expression was validated in CRC tissues using Quantitative real-time PCR (qRT-PCR), immunohistochemical (IHC) analysis, and western blot. Overexpression and silencing models were constructed using plasmid and lentivirus transfection. Cell viability, proliferation, and migration in vitro were applied to evaluate the protumoral effects of ENO3 on CRC. RNA sequencing and GO enrichment analysis of differentially expressed genes (DEGs) were performed to explore the underlying molecular mechanisms of ENO3 in CRC progression. The ATP and lactate production level were detected to assess cell glycolysis.
Facile Synthesis, Characterization, Catalytic and Photocatalytic Activity of Multiferroic BiFeO3 Perovskite Nanoparticles
Authors: Sukumar M , Mathankumar M. , Dash Chandra Sekhar , Sundararajan M. , Ubaidullah Mohd , Shaikh Shoyebmohamad F. , Sutha A. , Raza MdKausar , Dhanraj JoshuvaArockia 
Published: January 2022

Abstract
We report the synthesis of multiferroic BiFeO3 perovskite nanoparticles using the microwave combustion technique. Phase evolution is investigated by X-ray diffraction (XRD), which confirms that the formation of a secondary α-Bi2O3 phase with a monoclinic structure along with the existing rhombohedral (BiFeO3) structure. The average crystalline size has been found at 50 nm. The optical band gap was calculated from the Tauc’s plot it has been found 2.18 eV, as measured by diffuse reflectance spectroscopy (DRS). The appearances of Fourier transform infrared spectroscopy (FT-IR) absorption bands at 550 and 444 cm-1 were correlated to the rhombohedral stretching modes of bismuth ferrite nanostructure. The morphology observations using scanning electron microscopy (SEM) showed the formation of nanosized grains with pores. Energy-dispersive X-ray analysis (EDX) was done to confirm the extent of Bi3+, Fe3+, and O2- in the samples. The magnetization-Field (M-H) hysteresis curves recorded from the vibrating sample magnetometer (VSM) revealed the appearance of ferrimagnetic behavior at room temperature. The specific surface area characterized by N2 adsorption-desorption isotherm is found 44.86 m2 g-1 using Brunauer-Emmett-Teller (BET) technique. The as-fabricated BiFeO3 perovskite nanoparticles were investigated for their superior catalytic activity in two applications, which include (i) the conversion of glycerol to formic acid in a selective liquid phase batch reactor at atmospheric pressure. This bismuth-based nanoparticles exhibit as an efficient multifunctional catalyst with high conversion and selectivity efficiency around 99.2% and 98.5%, respectively, (ii) the photocatalytic degradation of rhodamine B under visible light irradiation is found maximum efficiency (99.9%), when a small amount of H2O2 was added during photocatalysis, indicating the samples possessed photo-Fenton like catalytic activity. Finally, we concluded that the BiFeO3 perovskite nanoparticles' high performance in future multifunctional devices is demonstrated by the simultaneous enhancement of catalytic and photocatalytic activities.