bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is It Possible to Run at High Speed Rotation, Above 3600 RPM, Asynchronous Electrical Submersible System? Myth, or Reality
Authors: Escobar Carlos , Valencia Victor , Castro Jhon , León Carlos , Pérez Laura , Serrano Juan , Ariza Iván 
Published: August 2022

Abstract
Over the years, Electro-Submersible Pumps have been considered one of the main Artificial Lift Methods in different fields of the world due to their versatility in handling different well fluids and wide ranges of flow production. However, in many cases, it is not possible to reach the maximum potential of a well due to the perception of avoiding operating the Asynchronous Motors of the ESP system above 3600 RPM because it may induce failures.
During the operation of ESP systems, situations may occur during which it may be necessary to operate the motor above the standard frequency 50/60 Hz (3000/3600 RPM) and take advantage of the maximum potential of the well without requiring an immediate intervention. These situations include but are not limited to well productivity index differing from the expected, loss of mechanical transmission in some of the system components, or decrease in efficiency due to wear associated with equipment operation.
In the ESP industry there is a design practice of operating frequencies no higher than 60 Hz (3600 RPM) to avoid possible equipment failures associated with rotational instability, high temperatures or poor lubrication in the internal components due to greater friction at higher speeds. The recommended practices or international standards do not have an operating speed limit reference apart from the restrictions that can normally occur in the system, so there is uncertainty in the operating limits of these systems.
The main objective of this paper is to verify or disprove the theory of the high speed rotation in 500-series ESPs with Asynchronous Motors (standard technology) with a specific number of wells that were operated at speeds above 4000 RPM.
High-level expression of soluble human epidermal growth factor in Escherichia coli
Authors: Liu Kun , Wang Feng-Qing , Zhao Ming , Gao Bei , Xu Hong , Wei Dongzhi 
Published: August 2022

Abstract
Human epidermal growth factor (hEGF) has multiple biological functions, and it is a very expensive polypeptide with attractive market prospects. However, the production of hEGF needs for high cost to manufacture polypeptide demands reinvestigations of process conditions so as to enhance economic benefits. Improving the expression of soluble hEGF is the fundamental method to reduce the cost. In this study, a non-secretion engineered strain of biosynthesized hEGF was constructed, using plasmid pET-22b(+) in Escherichia coli. Preliminary fermentation and high cell density cultivation were carried out in shake flasks and in a 5 L bioreactor, respectively. A high yield of 98 ± 10 mg/L of soluble hEGF and a dry cell weight (DCW) of 6.98 ± 0.3 g/L were achieved in shake flasks. Then, fermentation conditions were optimized for large-scale production, while taking into consideration the expensive equipment required for cooling and conforming to industrial standards. A yield of 285 ± 10 mg/L of soluble hEGF, a final cell density of 57.4 ± 2 g/L DCW (OD600 = 141.1 ± 4.9), and hEGF productivity of 14.3 mg/L/h were obtained using a bioreactor at 32 ℃ for 20 h. The production method developed in this study for the biosynthesis of soluble hEGF is efficient and inexpensive.
Organic geochemistry characteristics of the Permo-Carboniferous coals from the Huainan coalfield, East China: implications for paleoenvironmental changes
Authors: Cai Jiawei , Wang Ruwei , Huang Qing , Cai Feixuan , Yang Qiling 
Published: August 2022

Abstract
Climate–vegetation interactions belong to the most intriguing relationships in Earth’s history, especially for the late Paleozoic ice age with multiple icehouse-greenhouse climate transitions. Biomarkers preserved in coals provide evidence for potential taxonomic relationships among taxa with large scale paleoclimatic and paleofloral interpretations. In this study, we decipher the interactions among Permo-Carboniferous paleoclimate change and floral turnovers in the North China Craton. This is achieved by comprehensive investigation of the stratigraphic distribution of biomarkers in cored coals from Huainan coalfield, North China. Results indicated that the concentration and composition of biomarkers in coal, though irrevocably altered by diagenetic transformation and epigenetic activities, are closely related to the source of organic matter, depositional environment, and/or post-depositional change. The estimated molecular parameters of aliphatic and aromatic compounds are consistent with the maturity stage determined by Rock-Eval and organic petrology, which characterized the coals as high volatile bituminous A/B primarily consisting of mixed organic matters originating from marine algae and terrestrial higher plants. Enhanced maturity of coal results in the isomerization reactions of sterane isomers, tricyclic and tetracyclic diterpanes due to thermal stress and obscures the relationships between biomarker parameters and their biological precursors. The n-alkane distributions, though profoundly affected by thermal maturation, point towards the terrestrial higher plant inputs reflecting by the odd predominance of long-chain n-alkanes. The parallel stratigraphic patterns of C30 βα/(αβ + βα) with those of Pr/Ph ratios suggest that redox conditions is likely responsible for epimerization reactions of the hopane that converting βα to geologically preferred αβ isomers. The abrupt increase of 4- to 7-ring PAHs at the boundary of Lower and Upper Shihezi formations argue for a period of enhanced wildfire frequency. Moreover, the stratigraphic distribution patterns of biomarker parameters are consistent with the floral turnovers from initial warm and humid climate adaptive plant groups to semi-arid adaptive plant groups, and finally to dry climate adaptive plant groups.
The integration of rapid qualitative research in clinical trials: Reflections from the ward-based goal-directed fluid therapy (GDFT) in acute pancreatitis feasibility trial
Authors: Vindrola-Padros Cecilia , Froghi Farid , Gopalan Vignesh , Maruthan Sachan , Filipe Helder , McNeil Margaret , Garcia Sara Mingo , Davidson Brian 
Published: August 2022

Abstract
Purpose
There has been an increase in the integration of qualitative studies in randomised controlled trials. However, many of these studies do not produce findings at a time when they can be used to inform decision-making processes on trial design and delivery, allowing for changes in real time. The purpose of this article is to address this gap by reflecting on our experience of carrying out a rapid qualitative study during a feasibility trial of goal-directed fluid therapy (GDFT) in patients with acute pancreatitis.
Methods
The study was designed as a rapid feedback evaluation and combined interviews with staff and patients who took part in the trial with a telephone audit of patients who declined participation.
Findings
: The rapid qualitative study pointed to common problems in trial recruitment among multiple sites, where lack of engagement of clinical teams across sites might impact negatively on patient recruitment. The study also pointed to issues encountered while implementing the trial that will be useful for informing the potential scale-up of the trial in the near future.
Conclusions
The rapid qualitative design can generate findings at a time when these can be used to inform changes in trial design and delivery. Additional research is required to explore the implementation of this research design in other settings and trial designs.
Capital Control and Heterogeneous Impact on Capital Flows
Authors: sanyal Anirban 
Published: August 2022

Abstract
Capital control is used as policy toolkit for safeguarding domestic economy from the volatility of capital flows. However, the effect of capital control is rather far fetched as the signaling effect of capital control can moderate investor’s outlook about domestic economy and thereby outweighs the intended effect. The spillover effect, on the other hand, modulates the capital flows to other countries when one country increases capital account restrictions. Further, the effect of capital control can have varying impact on capital inflows to different sectors as recent studies indicate heterogeneity in the drivers and nature of capital inflows to different institutional sectors. With the background, the paper analyzes the heterogeneous direct and spillover effect of capital control on gross capital flows across three major institutional sectors namely public, banks and corporate. The paper validates the possible heterogeneity in the effect of capital control on the capital inflows to these institutional sectors using spatial econometric models. The paper observes that the direct effect of capital control moderates portfolio inflows to public sector whereas the effect is insignificant on portfolio inflows to banks and corporate sector. Further, the paper observes that the spillover effect of capital control is broad-based i.e. equally prevalent on all sectors. The paper explains the heterogeneity in the capital control effects by introducing signaling effect in a portfolio choice model. The paper argues that the heterogeneous direct effect is driven by private signals of capital control received by the investors about the state of economy whereas the spillover effect of capital control is mainly driven by the hedging and search for better returns. The paper extends the existing literature of capital controls by reviewing the sectoral heterogeneity in the capital flows.
Creating an appropriate model of advance care planning for women with breast cancer, Delphi method
Authors: Caineiro Yaritza , Martinet Axelle , Blanchard Clara , Mauhourat Julia , Besnier Marc , Freche Bernard 
Published: August 2022

Abstract
Introduction:
Breast cancer, the most common cancer in women, affects young women. Advance directives allow the expression of one's wishes concerning end of life wishes but they are poorly drafted. General practitioners report, in fact, real difficulties in talking about the end of life.
Purpose
This study aims to propose a model of advance directives that facilitates the exchange between the doctor and a breast cancer patient.
Methods
It is the result of a consensus of experts, obtained by the Delphi method. Twenty-one experts were included. A Delphi survey consisting of three rounds. The panel of experts were caregivers in contact with breast cancer patients, a Doctor of Sociology, deputy director of the National Center for Palliative Care and End of Life, as well as a former patient in remission from cancer. breast, association president. This new innovative model obtained takes into account in a more intimate way the moral values, the wishes of care and their environment.
Results
The first round lasted three weeks. Of the 29 questions posed to the experts, 20 were deemed relevant by over 70% of the experts. The second round lasted four weeks. Nine reworded questions were addressed to the experts. Eight of them were validated. The last question was validated in the third round. All experts responded to all three rounds.
Conclusion
The model created is a tool to create a more efficient dialogue between the physician and the patient. It is intended to be a faithful testimony of the patients’ wishes.
A study of high frequency laser beam oscillation induced keyhole dynamics in laser powder bed fusion additive manufacturing process
Authors: Wu Ziheng , Tang Guannan , Clark Samuel , Meshkov Andrey , Roychowdhury Subhrajit , Gould Benjamin , Ostroverkhov Victor , Adcock Thomas , Duclos Steven , Fezzaa Kamel , Immer Christopher , Rollett Anthony 
Published: August 2022

Abstract
The metal additive manufacturing (AM) industry is actively developing instruments and strategies to enable higher productivity, optimal build quality, and controllable as-built microstructure. One of the beam controlling techniques, laser oscillation has shown potential in all these aspects in laser welding; however, few attempts have been made to understand the underling physics of the oscillating keyholes/melt pools which are the prerequisites for the these strategies to become a useful tool for laser-based AM processes. To address this gap, we utilized a synchrotron-based X-ray operando technique to image the dynamic keyhole oscillation in Ti-6Al-4V using a miniature laser powder bed fusion setup. We found good agreement between the experimental observations and simulations performed with a validated Lattice Boltzmann multi-physics model. The study revealed the continuous and periodic fluctuations in the characteristic keyhole parameters that are unique to the oscillating laser beam processing and responsible for the chevron pattern formation at solidification. Despite the intrinsic longer-range fluctuation, the oscillating technique displayed potential for reducing keyhole instability, mitigating porosity formation, and altering surface topology. The results provide important insights about the dynamics of the oscillating keyholes which are valuable guidelines for the future development and application of this technique.
Yield model and yield table construction in Albizia (Albizia lebbeck (L.) under Western Agro Climatic Zone of Tamil Nadu, Southern India
Authors: Arunachalam Balasubramanian , Natarajan Hari Prasath , Satheesan Anjali , Ganesan Swathiga , Suthandhirajan Radhakrishnan , Muthuswamy Sivaprakash , Vellan Manimaran , Selvaraj Navaneetha Krishnan 
Published: August 2022

Abstract

The woody legume,Albizia lebbeck, is native to tropical Asia, Africa and Northern Australia. Owing to its multi utility, it is widely planted as block plantation and in boundary across community lands. A field study was conducted by measuring biometric value of trees,viz., height, diameter at every 2 meters height using tree telescope. Besides biometric attributes, age was assessed in order to correlate the age-wise yield. The study was carried out in western agro-climatic zone of Tamil Nadu in different age classes of trees,viz., 1–5 years, 6–10 years, 11–15 years, 16–20 years, 21–25 years, 26–30 years and 31–35 years. The uniqueness of the present study is that, the yield model construction for Albizia was done for standard stem timber (Volume at diameter 20 cm overbark) and standard stem small wood (Volume between diameter limits of 20cm and 5cm both measured overbark). After computing the data and subjected for multiple linear regression analysis, the model was constructed, so as to develop yield table accurately for Albizia without the help of form factor. The study result developed two exclusive yield models namely standard stem timber yield model for Albizia was Y= (-1.212) + (-0.012* Age) + (6.506*Diameter) and standard stem small wood was Y= (-0.945) + (-0.045*Age) + (2.769*Diameter) + (0.262* No. of Branches). The prominence of the present study is that yield table computation for Albizia is possible accurately without destructive analysis and the cumbersome process of developing form factor.
Examining self-reported aberrant behavior among Lebanese drivers using the Driver Behavior Questionnaire (DBQ)
Authors: Youssef Dalal , Salameh Pascale , Abou-Abbas Linda , Salmi Louis-Rachid 
Published: August 2022

Abstract
The Driver Behavior Questionnaire (DBQ) is one of the most used tools for measuring self-reported aberrant driving behavior. Several versions of the DBQ exist varying in terms of the item number and factor structure. This study aimed to investigate the psychometric properties and the factorial structure of the DBQ for assessing aberrant behavior among Lebanese drivers and to examine the relationship between DBQ subscales and socio-demographic characteristics and traffic-related variables. A cross-sectional study was conducted among Lebanese drivers aged more than 18 years old from all Lebanese governorates. Using a convenience sampling technique, data were collected using an anonymous Arabic, self-administered questionnaire that includes socio-demographic characteristics, and the Arabic Lebanese version of the DBQ (DBQ-L). Statistical analyses were performed using SPSS version 24 and Amos software. Exploratory factor analysis (N = 568) and confirmatory factor analysis (N = 534) were performed to test the factorial structure of the DBQ-L. Linear regression analyses were performed to examine the association between DBQ-L, sociodemographic and relevant traffic variables. A total of 1102 drivers aged between 18 and 82 years participated in this study with an average age of 34.64 (SD = 12.33). Of the total, 68.4% of participants were male having an average driving experience of 3.46 years (SD = 12.33). The DBQ-L total scale revealed high internal consistency (Cronbach’s α = 0.892). Exploratory and confirmatory factor analysis supported the original four-factor structure reflecting aggressive violations, ordinary violations, errors, and lapses dimensions. Aggressive violations had the highest mean among all the DBQ subscales. Men were found to have a higher tendency to commit aggressive and ordinary violations than females. Besides, the ordinary violation score was positively associated with younger age and increased mileage. All DBQ subscales decreased with the highest educational level. Being a professional driver increased the likelihood of drivers reporting violations, errors, and lapses, and increased mileage amplified the tendency of displaying aggressive violations and lapses when driving. However, errors decreased for young drivers and increased with age and larger driver experience. The cross-cultural adapted version of the DBQ was found to be a reliable and valid tool for assessing aberrant behaviors among Lebanese drivers. Further studies are needed to examine the ability of DBQ to predict road accidents.
Sequencing and analysis of the complete mitochondrial genomes of Toona sinensis and Toona ciliata reveal evolutionary features of Toona
Authors: Li Youli , GU Min , Lin Jiana , Xiao Xingcui , Zhou Wei 
Published: August 2022

Abstract
Toona is a critical genus in the Meliaceae, and the plants of this group are an asset for both restorative and restorative purposes, the most flexible of which are Toona sinensis and Toona ciliata. To concentrate on the advancement of mitochondrial genome variety in Toona sinensis and Toona ciliata, the mitochondrial genomes of the two species were sequenced in high throughput independently, after de novo assembly and annotation to construct a mitochondrial genome map for comparison in genome structure, along with ML phylogenetic analysis with seven other Toona relatives. Results show:(1)The mitochondrial genome of Toona ciliata is 683,000 bp in length, with 45.40% GC content and circular structure, and contains 38 protein genes plus 33 transfer RNA genes, of which 33 tRNAs encode 20 amino acids. AT preference exists for MtDNA, TTT is the most frequently encountered codon, appearing 413 times, as well as the largest codon corresponding to The codon encoding the equivalent of Phe, which had the maximum content. (2)Mitochondrial genome of Toona sinensis is 638482bp in entire length, with 45.56% GC content and a circumscribed architecture, it encodes 38 protein genes and 34 transfer RNA genes, among 34 tRNAs embodying 20 amino acids. AT is preferred by mtDNA, whereas TTT, which is utilized 412 times quite often and seems to have the maximum Phe concentration, is the most frequently employed codon. (3)Both the mitochondrial genomes of Toona sinensis and Toona ciliata are highly preserved in terms of structural and functional genes, while the main variability is reflected in the length of tRNA and the number of genes. (4) In the ML evolutionary tree, Toona sinensis and Toona ciliata clustered individually into a small branch with 100% support, reflecting two species of Toona are very similarly related to each other. This research provides a basis for the exploitation of Toona sinensis and Toona ciliata in terms of medicinal, edible, and timber resources to avoid confusion; at the same time, it can explore the evolutionary relationship between the Toona and related species, which does not only have an important practical value, but also provides a theoretical basis for future hybrid breeding of forest trees, molecular markers, and evolutionary aspects of plants, which has great scientific significance.
Genetic variation in correlated regulatory regions of immunity
Authors: Avalos Diana , Rey Guillaume , Ribeiro Diogo M. , Ramisch Anna , Dermitzakis Emmanouil , Delaneau Olivier 
Published: August 2022

Abstract
Studying the interplay between genetic variation, epigenetic changes and regulation of gene expression in immune cells is important to understand the modification of cellular states in various conditions, including immune diseases. Here, we built cis maps of regulatory regions with coordinated activity – Cis Regulatory Domains (CRDs) – in neutrophils, monocytes and T cells. For this, we leveraged (i) whole-genome sequencing (WGS), (ii) chromatin immunoprecipitation sequencing (ChIP-seq), (iii) DNA methylation (450k arrays), and (iv) transcriptional profiles (RNA-seq) from the BLUEPRINT consortium, for up to 200 individuals.
Our study uncovers 9287, 7666 and 5480 histone CRDs (hCRDs) and 6053, 6112, 5701 methyl CRDs (mCRDs) in monocytes, neutrophils and T-cells, respectively. We discovered 15294 hCRD-gene and 6185 mCRD-gene associations (5% FDR). Only 33% of hCRD-gene associations and 37% of mCRD-gene associations were shared between cell-types, revealing the dynamic nature of regulatory interactions and how similarly located regulatory regions modulate the activity of different genes on different cell types. We mapped Quantitative Trait Loci associated with CRD activity (CRD-QTLs) and found that 89% and 70% of these hCRDs and mCRDs are under genetic control highlighting the importance of genetic variation to study the coordination of cellular regulatory programs. We found CRD-QTLs to be enriched in cell-type-specific transcription factor binding sites, such as SPI1, STAT3, RFX1, SOX4, ATF3 for neutrophils and monocytes and TCF4 and BCL11A for T-cells, in line ​​with the Human protein Atlas.
We integrated PCHi-C data, which showed that most significant associations discovered within gene-CRD associations and co-expressed genes associated with the same CRD, involving large genomic distances, tend to happen between genomic regions in close spatial proximity. Finally, we mapped trans regulatory associations between CRDs, which enabled the discovery of 207 trans-eQTLs across cell types. Overlapping our hits with trans eQTLs from eQTLGen Consortium meta-analysis in whole blood revealed 81 trans-eQTLs shared between the two studies. Overall, we show that mapping functional regulatory units using population genomics data allows discovering important mechanisms in the regulation of gene expression in immune cells and gain a greater understanding of cell-type specific regulatory mechanisms of immunity.