bluuebunny/crossref_metadata_2025_split huggingface dataset:
CEP55 3’-UTR promotes epithelial–mesenchymal transition and enhances tumorigenicity of bladder cancer cells by acting as a ceRNA regulating miR- 497-5p
Authors: Yang Chenglin , Yang Yue , Wang Wei , Zhou Wuer , Zhang Xiaoming , Xiao Yuansong , Zhang Huifen 
Published: May 2022

Abstract
Background: Centrosomal protein 55 (CEP55) is implicated in the tumorigenesis of bladder cancer (BC) but the detailed molecular mechanisms are unknown. We aim to develop a potential competing endogenous RNA (ceRNA) network related with CEP55 in BC.Methods: We first extracted the expression profiles of RNAs from The Cancer Genome Atlas (TCGA) database and used bioinformatic analysis to establish ceRNAs in BC. Real-time quantity PCR (RT-qPCR) and immunohistochemical analysis were performed to measure CEP55 expression in different bladder cell lines and different grades of cancer. Bioinformatics analysis and luciferase assays were conducted to predict potential binding sites among miR-497-5p, CEP55, parathyroid hormone like hormone (PTHLH) and high mobility group A2 (HMGA2). Tumor xenograft model was used to show the effect of CEP55 3’-UTR on cisplatin therapy. Bioinformatics analysis, luciferase assays, and 5' rapid amplification of cDNA ends (5’RACE) were to explore the function of CEP55 3’-untranslated region (3’-UTR) on targeting miR-497-5p. Western blot and immunofluorescence assays were to detect the epithelial–mesenchymal transition (EMT) induction of CEP55 3’-UTR.Results: CEP55 expression as well as the expression levels of the oncogenic proteins PTHLH and HMGA2 were upregulated in BC cells while miR-497-5p was downregulated. Low miR-497-5p expression and high CEP55 and HMGA2 expression levels were associated with more advanced tumor clinical stage and pathological grade. Overexpression of the CEP55 3’-UTR promoted the proliferation, migration, and invasion of the EJ cell line in vitro and accelerated EJ-derived tumor growth in nude mice, while inhibition of the CEP55 3’-UTR suppressed all of these oncogenic processes. In addition, CEP55 3’-UTR upregulation reduced the cisplatin sensitivity of BC cell lines and xenograft tumors. Bioinformatics analysis, luciferase assays, and 5’RACE suggested that the CEP55 3’-UTR functions as a ceRNA targeting miR-497-5p, leading to miR-497-5p downregulation and disinhibition of PTHLH and HMGA2 expression. Further, CEP55 downregulated miR-497-5p transcription by promoting NF-kB signaling. In turn, PTHLH and HMGA2 activated p38MAPK and ERK 1/2 pathways, and induced EMT of BC cells. Conclusions: These results suggest that a ceRNA regulatory network involving CEP55 upregulates PTHLH and HMGA2 expression by suppressing endogenous miR-497-5p in bladder cancer.
pregnancy outcome and follow-up of offspring of donor oocytes recipient from PCOS patients
Authors: JIANG YAN , YUAN JING-CHUAN , SONG GE , ZHANG XU-HUI , MIAO SUI-BING , WU XIAO-HUA 
Published: May 2022

Abstract
Background
The use of donated oocytes (DO) for in vitro fertilization(IVF) treatment in patients with infertility is generally recognized, and women with polycystic ovarian syndrome (PCOS) are able to participate in oocyte donation programs as donor patients. However, the pregnancy outcomes and follow-up of offspring of PCOS receptor is unclear.
Objective
Comparing the pregnancy outcomes and follow-up of offspring in PCOS and non-PCOS receptor.
Design
: Retrospective cohort study.
Methods
A total of 62 patients who had undergoing oocyte reception program were separated into two groups: Group I, PCOS oocyte receptor (𝑛=30); Group II, non-PCOS receptors (𝑛=32). Medical records were reviewed and rates of fertilization, cleavage, high quality embryos and blastocysts were compared between PCOS and non-PCOS receptors. Rates of implantation, pregnancy, ectopic pregnancy, early abortion, multiple pregnancy, and offspring outcome were calculated in first single vitrified-warmed blastocyst transfer (SVBT) were analyzed between PCOS and non-PCOS receptors.
Results
The average recipient age from PCOS and non-PCOS patients were 36.3 ± 2.6 and 36.2 ± 2.8, respectively. Rates of fertilization, cleavage, high quality embryos and blastocysts were not significantly different. Rates of implantation, pregnancy, ectopic pregnancy, early abortion, and multiple pregnancy were not significantly different in SVBT between PCOS and non-PCOS receptors. The incidences for complications such as PIH, or gestational diabetes between PCOS and non-PCOS receptors were similar (11.8% vs.11.1%, 5.9% vs.5.5%; P > 0.05). Preterm birth were similar (11.8% vs.16.7%, P > 0.05). Donor oocytes were more likely to deliver by caesarean section (80.0% vs. 86.7%: P > 0.05). And the mean gestational age, as well as the birth weight, and height were comparable for both groups in full-term delivery.
Conclusions
There was no difference in the pregnancy outcomes and follow-up of offspring between PCOS and non-PCOS receptor.
ZnO and Ni-doped ZnO photocatalysts: Synthesis, characterization and improved visible light driven photocatalytic degradation of methylene blue
Authors: Ahmad Iqbal , Aslam Mehwish , Jabeen Uzma , Zafar Muhammad Nadeem , Malghani Muhammad Najam Khan , Alwadai Norah , Alshammari Fwzah H. , Almuslem Amani Saleh , Ullah Zahid 
Published: May 2022

Abstract
The nickel modified zinc oxide (ZnO) photocatalysts with nominal composition of Zn1 − xNixO (x = 0.0–0.5) were synthesized by wet chemical approach. Optical studies were performed using Fourier transformed infrared (FTIR), electronic spectroscopy and X-ray diffraction (XRD) methods. Scanning electron microscopy (SEM) complimented with Energy Dispersive X-ray (EDX) was used to study the morphology and chemical composition of prepared photocatalysts. A significant hypsochromic shift has been observed with respect to un-doped ZnO nanocatalyst due to quantum confinement effect. Subsequently, the band gap has been tuned to the region of lower wavelength. X-ray results reveal that ZnO nanocatalyst are in hexagonal crystalline form. In addition, the effect of nickel impurity on photocatalytic activity of ZnO nanocatalysts in degradation of methylene blue (MB) was also investigated. Degradation results showed that the MB was degraded more effectively by Ni-doped ZnO photocatalysts due to large band gap under visible light irradiation.
A Meta-analysis of Internet-based Cognitive Behavioral Therapy for Military and Veteran Populations
Authors: Liu Jenny J.W. , Ein Natalie , Forchuk Callista , Wanklyn Sonya G. , Ragu Suriya , Saroya Samdarsh , Nazarov Anthony , Richardson J. Don 
Published: May 2022

Abstract
Background
Military and veteran populations are unique in their trauma exposures, rates of mental illness and comorbidities, and response to treatments. While reviews have suggested that internet-based Cognitive Behavioral Therapy (iCBT) can be useful for treating mental health conditions, the extent to which they may be appropriate for military and veteran populations remain unclear. The goals of the current meta-analysis are to: (1) substantiate the effects of iCBT for military and veteran populations, (2) evaluate its effectiveness compared to control conditions, and (3) examine potential factors that may influence their effectiveness.
Methods
This review was completed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) reporting and Cochrane review guidelines. The literature search was conducted using PsycInfo, Medline, Embase, and Proquest Dissertation & Theses on June 4, 2021 with no date restriction. Inclusion criteria included studies that: (1) were restricted to adult military or veteran populations, (2) incorporated iCBT as the primary treatment, and (3) evaluated mental health outcomes. Exclusion criteria included: (1) literature reviews, (2) qualitative studies, (3) study protocols, (4) studies that did not include a clinical/analogue population, and (5) studies with no measure of change on outcome variables. Two independent screeners reviewed studies for eligibility. Data was pooled and analyzed using random-effects and mixed-effects models. Study data information were extracted as the main outcomes, including study condition, sample size, and pre- and post-treatment means and standard deviations for all assessed outcomes. Moderator information were also extracted, and included demographics information, the types of outcomes measured, primary condition targeted, concurrent treatment, drop pout rate, and format, length, and delivery of intervention.
Results
A total of 20 studies and 144 samples of data were included in the meta-analysis. The pooled effect size showed a small but meaningful effect for iCBT, g = 0.63, SE = 0.05, 95% CI (0.54, 0.73), Z = 13.14, p < .001.
Conclusions
Findings from the meta-analysis lend support for the use of iCBT with military and veteran populations. Conditions under which iCBT may be optimized are discussed.
Emergence of Nutrigenomics and dietary components as a complementary therapy in cancer prevention
Authors: Srivast Shambhavi , Dubey Ankit Kumar , Madan Reecha , Bala Rajni , Gupta Yugam , Singh Bahadur , Kumar Suresh 
Published: May 2022

Abstract
Cancer is an illness characterized by abnormal cell development and the capability to infiltrate or spread to rest of the body. When this abnormal development progresses in solid tissues, such as an organ, muscle, or bone it is known as tumour, which can spread to other regions of the body via the blood and lymphatic systems. Nutrition is a critical and immortal environmental component in the development of all living organisms encoding the relationship between a person's nutrition and their genes. These interactions have a significant impact on the pharmacokinetic properties of bioactive dietary components, as well as their site of action/molecular targets. They have the ability to modify gene expression and persuade alterations in DNA and protein molecules, which is researched scientifically in nutrigenomics. Nutrigenomics encompasses nutrigenetics, epigenetics, and transcriptomics, as well as other “omic” disciplines like proteomics and metabolomics, to explain the vast disparities in cancer risk among people with roughly similar life style. Clinical trials and researches have evidenced that alternation of dietary habits is potentially one of the key approaches for reducing cancer risk in an individual. In this article, we will target how nutrigenomics and functional food work as preventive therapy in reducing the risk of cancer.
iTLS™ model for increasing operational efficiency: Application in an energy and telecommunications systems industry
Authors: De Lucia Walison Mathias , Braga Washington Luis Moreira 
Published: May 2022

Abstract
Theory of Constraints, Lean Manufacturing, and Six Sigma are methodologies that have already brought numerous benefits regarding system efficiency. Their applications range go from operations management to service levels, revolutionizing the market and boosting the economy. However, the market demand is extremely high and aiming for continuous improvement as a way to always seek to prosper, integration models related to these methodologies have been proposed. As a way to amplify their benefits of use, the objective of this article is to propose and execute a logical application roadmap through a model that integrates the methodologies of Theory of Constraints, Lean Manufacturing and Six Sigma, called iTLS™, a model that is little studied in the literature. Therefore, the action research method was used where the limiting factors of the process were highlighted and corrective actions were outlined that resulted in a significant improvement in operational performance. The process of improving operational performance follows a logical sequence of tool application developed through the proposal of a methodology using the iTLS™ model and using as metrics the OEE (Overall Equipment Effectiveness), MTBF (Mean Time Between Failures) and MTTR (Mean Time To Repair) indicators.
A simple denoising approach to exploit multi-fidelity data for machine learning materials properties
Authors: Rignanese Gian-Marco , Liu Xiaotong , De Breuck Pierre-Paul , Wang Linghui 
Published: May 2022

Abstract
Machine-learning models have recently encountered enormous success for predicting the properties of materials. These are often trained based on data that present various levels of accuracy, with typically much less high- than low-fidelity data. In order to extract as much information as possible from all available data, we here introduce an approach which aims to improve the quality of the data through denoising. We investigate the possibilities that it offers in the case of the prediction of the band gap relying on both limited experimental data and density-functional theory relying different exchange-correlation functionals (with an increasing amount of data as the accuracy of the functional decreases). We explore different ways to combine the data into training sequences and analyze the effect of the chosen denoiser. Finally, we analyze the effect of applying the denoising procedure several times until convergence. Our approach provides an improvement over existing methods to exploit multi-fidelity data.
Respiratory microbiome dysbiosis and temporal dynamics in individuals with asthma.
Authors: Megremis Spyridon , Yap Chuan Fu , Tapinos Avraam , Kritikou Maria , Wardzyńska Aleksandra , Busby Joseph , Rovina Nikoletta , Chałubiński Maciej , Xepapadaki Paraskevi , Muldoon Mark , Gilman R. Tucker , Robertson David , Papadopoulos Nikolaos G 
Published: May 2022

Abstract
Microbiome dysbiosis across the respiratory tract has been implicated in the development and exacerbations of asthma. Little is known regarding the short-term temporal variability of the respiratory microbiome in health and asthma. In this study, we evaluate six scenarios of dysbiosis by investigating the inter- and intra-individual variability of the nasopharyngeal microbiome, measured with metagenomic sequencing. We characterized 239 microbiome samples from thirteen healthy individuals and from seventeen patients with well-controlled mild to moderate persistent asthma collected during a one-month period in which patients experienced no asthma exacerbations or infections. We controlled for sex, age (range 5 to 38 years), population (Greece and Poland), and microbiome type, for multiple levels of species incidence (expanded to core). Microbiomes collected from the same individual at different time points were more similar than those collected from the same group (health or asthma), but some individuals’ microbiomes were more variable than others. Microbiomes drawn from asthma patients were less likely to resemble each other than the microbiomes of healthy individuals, suggesting increased inter-sample variability and susceptibility to stochastic changes in asthma. In addition, the microbiomes of asthma patients became increasingly dissimilar over time, suggesting increased intra-individual temporal dynamics. Finally, microbiomes of individuals with asthma were more diverse than those of healthy individuals, while species diversity was negatively associated with temporal change in both the asthma and health groups. We propose that the increased diversity, inter-sample variability and intra-individual temporal dynamics during stable disease state(s) are intrinsic properties of the microbial system in asthma and characterise dysbiosis. Our work has significant implications for the relevance of the respiratory microbiome in asthma.
Sustainable utilization and optimization of spray dried fermented pumpkin juice
Authors: Sharma Poorva , Kashyap Piyush , Kehinde Bababode Adesegun , Kaur Sawinder 
Published: May 2022

Abstract
Pumpkin, a nutrient rich vegetable with approximately 27 million tonnes production globally generates high amount of pumpkin waste. The need of the hour is the utilization of vegetable waste in sustainable way. Production of value added product is the possible solution for this problem. As fruit and vegetables have proved to be promising carriers for probiotics. Hence, in this work, research studies have been carried out on the effective utilization of pumpkin by developing non-dairy probiotic beverage. The extracted pumpkin juice was fermented by Lactobacillus fermentum NCDC 141 and microencapsulation of probiotic microorganisms was done by spray drying. Growth conditions of fermenting microorganism were optimized in MRS medium and pumpkin juice. Desirable viable cell count i.e more than 106 was observed with 10% inoculum size after 15 hr incubation time. Significant different was seen between properties of fermented and non-fermented pumpkin juice. Optimization of spray drying conditions to obtain maximum viable cells and DPPH scavenging activity and minimum moisture content and hygroscopicity was done by response surface methodology (RSM). Optimal conditions to get desirable characteristics were observed at 20% maltodextrin concentration and 140°C inlet air temperature. Fermented microencapsulated pumpkin powder showed 62.36% DPPH scavenging activity, 7.985 log CFU/ml viable cells, 2.413% moisture content and 16.324% hygroscopicity at optimized conditions. High encapsulation efficiency (89.00 ± 0.91%), water solubility index (87.59 ± 0.89%) and antioxidant activity (64.36 ± 1.28%) was seen for fermented powder. Further, scanning electron microscopy images showed adequate encapsulation of probiotic pumpkin juice with smooth and nearly spherical structure.
Care practices and short-term clinical outcomes of very and extremely low birth weight infants in Yangtze River Delta in China
Authors: Lyu Tianchan , Tao Yibo , Hua Wei , Li Liling , Tang Yunfei , Jin Yumei , Wang Yan , Ma Yuelan , Ji Futing , Dou Yalan , Cao Yun , Hu Xiao-jing 
Published: May 2022

Abstract
Background
Intensive care is of great significance for very and extremely low birth weight infants.
Objectives
To investigate the prevalence, care practices and motality of very and extremely low birth weight infants (VELBWI) in Yangtze River Delta in China.
Methods
A multi-center retrospective investigation study was conducted at five tertiary hospitals within the Yangtze River Delta in China from January to December 2017. Clinical data included the general characteristics of the infants and the mothers, clinical prognosis, care practices in NICUs was collected by trained research members.
Results
During the study period, 1059 VELBWIs were included. Infants with birth weight < 750g, 750-1000g, 1000-1250g and 1250-1500g accounted for 2.3%, 14.9%, 34.8% and 47.8%, respectively. Premature rupture of membranes (17.85%) was the main cause of premature delivery. The catheterization rates of umbilical vein catheterization (UVC) and peripherally inserted central catheter (PICC) were 25.02% and 64.4%, respectively. The duration of parenteral nutrition was 27.0 ± 19.5 d, the meantime of feeding tube indwelling was 36.2 ± 24.2 d. The corrected gestational age of the infants who reached full oral feeding was 35.8 ± 2.7 weeks. The breast feeding rate in the investigated infants was 61.9%. The mortality rate of preterm infants was 3.4%. The incidence of main complications BPD, PDA, ROP, NEC and sepsis were 24.9%, 29.9%, 21.72%, 9.4% and 13.3% respectively.
Conclusions
Maternal and infant care practices need to be improved in the very preterm births. This study provides a baseline for the improvement in the further study.
Comprehensive analyses of metabolism-related lncRNA for LUAD
Authors: Sun Xinti , Huang Xingqi , Sun Linao , Zhang Peng , Li Zesheng , Sun Xiaojuan , Zhang Peng 
Published: May 2022

Abstract
Lung adenocarcinoma(LUAD), as a tumor with high heterogeneity, strong invasiveness, and high degree of malignancy, has become a hot issue in contemporary medicine. With the deepening of tumor research, the metabolic reprogramming of tumor cells and the regulatory role of lncRNA in tumor progression have gradually become prominent. This article aims to explore the relationship between the above three. In this study, based on LUAD samples downloaded from TCGA and metabolism-related genes downloaded from KEGG database, 8 key metabolism-related lncRNAs (AC068228.1, LINC02390, AC123595.1, AC021016.1, LINC00707, AL132656.2, AL033397.2 and LINC00941) were screened for the construction of prognostic risk models. After multi-dimensional validation, this risk model proved to have good reliability and validity and was closely related to the immune status and drug tolerance of LUAD patients. In addition, to better clarify the molecular mechanism by which these key lncRNAs affect the LUAD process, we performed functional enrichment analysis and found their close relationship with hematopoietic cell lineage, lipid metabolism, and human humoral immunity. To increase the credibility of this study, we verified the expression levels of these eight lncRNAs in BEAS-2B, A549 and H1975 cell lines, and the results of PCR experiments were in good agreement with our risk model. Overall, the above studies aim to improve the understanding of LUAD and open up new ideas for guiding clinical treatment.
Robust and easy-to-use STR-based method for 15q11-q13 rearrangements identification
Authors: Chernushyn Sergey , Kravchenko Sergey , Patskun Erika , Hryshchenko Nataliya 
Published: May 2022

Abstract
The rearrangements of the 15q11-q13 region such as deletions, duplications, uniparental disomies (UPDs), and translocations caused different neurodevelopment phenotypes including Prader-Willy and Angelman syndromes, 5q11-q13 duplication syndrome, autism spectrum disorders. The aim of our study is to elaborate on the most universal, convenient, and informative molecular test for rapid detection of 15q11-q13 alterations in patients with suspected 15q associated disorders. Eight short tandem repeats (STR) markers within the 15q11.1-q13 locus and 1 STR from 15q21.1 in a combination with SNRPN gene qPCR allowed us to detect both copy number variations (deletions and duplications) and different types of UPDs, determining the origin and boundaries of the reorganization. The proposed strategy significantly improves medical genetic counseling for families - it allows the prediction of the disease severity, the estimation of genetic risks for families, and even applying the personalized approaches to therapy.
Expected Neutrino radiation from proton-photoninteraction in the TeV blazar TXS 0506+056
Authors: Dong qian , Zheng Yonggang 
Published: May 2022

Abstract
IceCube collaboration reported a ∼ 290 TeVhigh energy neutrino signal on 22 September 2017,which is usually referred to as IceCube-170922A. Thebest-fitting direction of IceCube-170922A is very closeto that of the TXS 0506+056 blazar. It means thatIceCube-170922A is consistent with the γ-ray flare of TXS 0506+056. To properly understand this phenomenon, we introduce a model involving synchrotronself-Compton and hadronic scenarios to explain the cooperative event. For the hadronic scenario, we assumethat the protons follow a power-law distribution witha high-energy cutoff under the effect of non-relativisticshock and synchrotron emission. We then consider thesynchrotron emission of protons and investigate the interaction of protons with the low-energy photons originated from the synchrotron emission. The protonphoton interaction is characterized by three main features: 1) the interaction produces Bethe-Heitler pairsand considers their synchrotron emission and inverseCompton scattering, 2) the decay of π0 produces γ-rays, 3) the production of π± is related to the presence of the muon neutrino, muon antineutrino, electron neutrino and electron antineutrino. Upon adopting the parametrization of the observed spectrum ofTXS 0506+056, we get the following results: 1) themodel is able to match the spectral energy distributionof TXS 0506+056, 2) the protons may indeed follow apower-law distribution with high-energy cutoff affectedby the shock in the jet, 3) the interaction of protonswith the low-energy photons may occur in the jet. Weargue that the high energy neutrino radiation IceCube-170922A may be understood in terms of the decay of π± produced in the interactions of protons with thesynchrotron photons in the jet, and the antineutrinosis equal to the neutrino spectrum nearly.