bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metal-organic framework [H2NMe2]3 [Tb(PDA)3] induces cytotoxic effects and changes DNA methylation-related gene expression in a human fibroblast cell line
Authors: Carrilo-Cocom Leydi Maribel , Juárez-Méndez Lucia , Rincon Susana , Rivera-Villanueva José María , Nic-Can Geovanny Iran , Zepeda Alejandro 
Published: March 2022

Abstract
The lanthanide metal-organic frameworks (lanthanide MOFs) may be utilized for a variety of environmental and human health applications due to its luminescent properties and high thermal and water stability. However, the cytotoxic and epigenetic effects that these produce in human cells are not known. Therefore, we evaluated the cytotoxic effects, internalization and changes in DNA methylation-associated gene expression induced by exposing human fibroblast cells to a Terbium-based MOF (Tb-MOF). For this purpose, cells were exposed to six concentrations (0.05 to 1.6 mg/mL) of [H2NMe2]3 [Tb(PDA)3] for 48 h. SEM, Confocal microscopy and EDS analysis were performed. Cytotoxicity was determined with the Crystal violet and MTT assays. The results demonstrated the internalization of Tb-MOF at concentrations as low as 0.05 mg/mL, as well as dose-dependent toxicity. Additionally, we detected significant changes in the gene expression levels of DNA methyltransferases and demethylases (important regulators of cell development) due to the presence of Tb-MOF, suggesting that Tb-MOF could generate epigenetic changes even at low concentrations. Results of our study may establish a foundation for future research attempting to develop and apply secure nanomaterials (e.g., MOFs) to minimize damage to the environment and human health.
Vision restoration in photoreceptor-degenerated mice and macaque monkeys using nanowires
Authors: Yang Ruyi , Zhao Peng , Wang Liyang , Feng Chenli , Peng Chen , Wang Zhexuan , Zhang Yingying , Shen Minqian , Shi Kaiwen , Weng Shi-Jun , Dong Chunqiong , Chen Yuqing , Zeng Fu , Zhang Tianyun , Fu Luoyin , Ma Ruoxi , Wang Fei , Jiang Su , Xu Wendong , Wang Gang , Zheng Gengfeng , Chen Aihua , Yuan Fei , Yuan Yuanzhi , Yan Biao , Zhang Jiayi 
Published: March 2022

Abstract
Photoreceptor degeneration diseases, such as retinitis pigmentosa and age-related macular degeneration, are major causes of blindness. Photovoltaic devices or materials activates retinal cells in response to light, hence are promising retinal prosthesis in treating blind patients. The restoration of image-forming vision with high sensitivity is the key. Here we demonstrated that Au nanoparticle-coated titania (TiO2-x) nanowire (NW) arrays can restore vision in blind mice and non-human primates with photoreceptor degeneration. We first showed that blind mice with subretinal implant of NW arrays were capable of detecting static, moving and flashing objects with low light intensity threshold for 22 months, having a visual acuity of 0.3 cpd (0.4 cpd in normal mice). Results in chronic in vivo calcium imaging in primary visual cortex (V1) collectively suggested the plastic change in V1 neurons as well as the improvement in visual information encoding for natural images after NW implant. What’s more, macaque monkeys with subretinal implant of NW arrays were capable of detecting an LED of 0.5º in diameter at 10 μW·mm-2 in visually-guided saccade experiments. Our findings opened up the possibility to utilize nanomaterials as artificial photoreceptors to ameliorate visual deficits of patients with photoreceptor degeneration.
Impacts of autofluorescence on fluorescence based techniques to study microglia
Authors: Zhang Haozhe , Tan Chen , Shi Xiaoyue , Xu Ji 
Published: March 2022

Abstract
Background
Microglia, the resident immune cells in the central nervous system, accrue autofluorescent granules inside their cytoplasm throughout their lifespan. In this report, we studied the impacts of autofluorescence on widely used fluorescence-based techniques to study microglia, including flow cytometry, immunofluorescence staining, and live imaging.
Results
The failed attempt of using fluorescein isothiocyanate (FITC) conjugated antibody to detect lymphocyte-activation gene 3 protein in microglia prompted us to compare the sensitivity of FITC, phycoerythrin (PE) and allophycocyanin (APC) conjugated antibodies to detect surface protein expression in microglia. We found that PE outperformed FITC and APC as the fluorophore conjugated to antibody for flow cytometry by overcoming the interference from microglia autofluorescence. To identify the location and source of microglia autofluorescence, we did confocal imaging and spectral analysis of microglia autofluorescence on fixed brain tissues, revealing that microglia autofluorescence emitted from cytoplasmic granules and displayed a multi-peak emission spectrum. We recommended removing autofluorescence by lipofuscin removing agents when staining intracellular proteins in microglia with immunofluorescence technique. On live brain slices, autofluorescent granules reduced the amplitudes of calcium signals in microglial somata calculated by F/F0 of GCaMP6s and thus needed to be excluded when selecting regions of interest.
Conclusions
In conclusion, autofluorescence is a critical factor to consider when designing experiments and interpreting results based on fluorescence-based techniques to study microglia.
Compliance with National Snakebite Treatment Guidelines in Rural Sri Lankan Hospitals: A Cluster Randomized Controlled Trial of a Brief Educational Intervention
Authors: Shahmy Seyed , Kularatne Senanayake AM , Gawarammana Indika B , Rathnayake Shantha S , Dawson Andrew H 
Published: March 2022

Abstract
BackgroundSnakebite is a global health problem predominately occurring in rural areas. In Sri Lanka, the majority of snakebites first present to smaller rural primary hospitals. Improving care delivered at rural hospitals has the potential to reduce morbidity and mortality from snakebites.ObjectiveIn this study, we evaluated whether an educational intervention would increase compliance with national snakebite treatment guidelines in primary hospitals.MethodsThe hospitals were randomized into educational intervention (n=24) and control group (n=20). The intervention hospitals received a brief educational intervention based on Sri Lankan Medical Association (SLMA) guidelines on management of snakebites. Control hospitals had free access to the guidelines but no additional promotion. Four outcomes were assessed; Pre and Post-test knowledge at the completion of a one-day workshop of educational intervention (Intervention group only), Improvement of quality of the patients’ medical records, appropriateness of transfers to higher hospitals, and quality of overall management graded by a blinded expert. The data were collected for a period of 12 months.ResultsAll case notes of snakebite hospital admissions were reviewed. There were 1021 cases in the intervention and 1165 cases in the control hospitals. Four hospitals in the intervention and 03 hospitals in the control groups did not have snakebite admissions and were excluded from the cluster analysis. The absolute quality of care was high in both groups. Post-test knowledge was improved (p< 0.0001) following the intervention group’s educational workshop. There was no statistical difference between the two groups in the documentation of clinical data in hospital notes (scores, p=0.58), appropriateness of transfers (p=0.68) both of which were at significant variance to the guidelinesConclusionRepresentative group education from primary hospitals had improved the immediate knowledge gaining. However, that failed to influence the record- keeping and treatment decisions, as all primary hospitals showed adequate compliance with the existing SLMA guidelines.Trial registrationThe study was registered with Sri Lanka Medical Associations’ clinical trial registry https://slctr.lk/trials . Reg. No SLCTR/2013/023. Registered: 30/07/2013.
Impacts of climate change on extreme precipitation in the upstream of Chushandian Reservoir, China
Authors: Gan Rong , Li Dandan , Chen Changzheng , Yang Feng , Ma Xichen 
Published: March 2022

Abstract
Analysis of trends in extreme precipitation events within a basin is essential to reliably predict future changes and to inform adaptation strategic planning. Based on daily data from eight stations in the upper basin of Chushandian Reservoir from 1957 to 2017, and Multi-model outputs from four Global Climate Models in CMIP6, we calculated the extreme precipitation index. Mann-Kendall method and linear trend analysis were used to examine the spatial and temporal variability of the extreme precipitation index. The results show that there is a clear downward trend in precipitation and precipitation intensity from 1957 to 2017 (represented by RX1day, RX5day, R10 mm, R20 mm, and SDII), and an upward trend in the annual scales of R95p, R99p, CWD, and CDD. The trend of the extreme precipitation index (represented by PRCPTOT, R20 mm, R95p, and SDII) in future periods is decreasing in 2020–2050 and then increasing in 2060–2100, with a significant increasing trend after the 2060s. These findings indicate that total precipitation, the frequency, and persistence of extreme precipitation are on the increase, and the future situation of extreme precipitation is severe, especially in autumn, followed by summer.
In Vivo and In Vitro Anti-schistosomiasis Effect of Garlic: A Systematic
Review
Authors: Shakib Pegah , Kalani Hamed , Ali Ali Safar Maken , Zebardastpour Mustafa , Moradpour Kobra , Ho Jeffery , Nazarabad Vahideh Heydari , Cheraghipour Kourosh 
Published: March 2022

Background:
Garlic (Allium sativum) is used as a natural supplement for the treatment of
various diseases and disorders because it has antibacterial, antiviral, antifungal, antiparasitic,
antioxidant, and anti-inflammatory properties. This systematic review aimed to evaluate in vitro and in
vivo effects of garlic against Schistosoma spp.

Method:
The current study was carried out according to the PRISMA guidelines and registered in the
CAMARADES-NC3Rs Preclinical Systematic Review and Meta-analysis Facility [SyRF] database.
The literature search was conducted using five databases, including Scopus, PubMed, Web of Science,
Embase, and Google Scholar, from January 2008 to January 2021. The search was restricted to articles
published in the English language. Syntax was performed based on each database tag.

Results:
Out of 2,600 studies, 10 met the eligibility criteria for review. The examined parasite in all
studies was Schistosoma mansoni. Ten studies (90%) were performed in vivo and one study in vitro.
Studies have shown that garlic compounds can activate immune system factors, thereby damaging the
parasite structure or its eggs.

Conclusion:
Given the increase in using plants in the treatment of many diseases and the fact that
plants can be a good alternative to chemical drugs in many cases, more comprehensive research is
needed to introduce effective medicinal plants to treat diseases such as schistosomiasis.
Thermal Exfoliated Graphite/Chitosan Modified Glassy Carbon Electrode for Cu(II) Ion Sensing
Authors: Le Nhan T.T. , Tran Hoang V. , Huynh Chinh D. , Nguyen Cuong D. , Phi Toan V. 
Published: March 2022

Aim:Here, we report a simple strategy for the preparation of thermally exfoliated graphite (EG) and its application to modify glassy carbon electrode (GCE) surfaces for electrochemical Cu2+ ion sensing.Method:The electrochemical Cu2+ sensor was constructed by a layer modification of a glassy carbon electrode (GCE) with exfoliated graphite (EG) and chitosan (CS) as a binder, and CS also supports a large number of -NH2 functional groups for Cu2+ capture.Result:Due to the creation of a three-dimensional (3D) structure, the EG/CS-coated GCE (EG/CS/GCE) electrode exhibited a higher sensitivity towards Cu2+ detection than that of modification by graphite/chitosan (GP/CS), chitosan (CS) or graphite intercalated compounds (GIC)/chitosan.Conclusion:The proposed method could detect Cu2+ in the range of 10 μM to 3 mM with a detection limit of 0.5 μM and sensitivity of 43.62 μA mM-1.
Green Synthesis of Silver Nanoparticles from Bark Extract of Terminalia
arjuna and their Application as Next Generation Antibacterial Agents
Authors: Sharma Shivesh , Singh Jaspreet , Perumal Venkatachalam , Singh Umrao , Tripathi Durgesh Kumar 
Published: February 2022

Background:
The antimicrobial properties of silver can be enhanced in the form of silver
nanoparticles due to their specific physical, chemical, and biological properties, thus enabling their
use in different antibacterial applications against antibiotic-resistant bacteria.

Objectives:
Present study was planned to evaluate the antibacterial activity of silver nanoparticles
synthesized from bark extract of Terminalia arjuna.

Methods:
Silver nanoparticles were synthesized using 80% methanolic extract of Terminalia arjuna
bark, followed by their characterization using UV-Visible spectroscopy, particle size analysis, and
atomic force microscopy. The antibacterial activity of synthesized silver nanoparticles was analyzed
against Escherichia coli MTCC1687, Pseudomonas aeruginosa ATCC9027, and Staphylococcus
aureus ATCC6538.

Results:
The silver nanoparticles were observed to inhibit microbial growth in a concentrationdependent manner (2-0.5mg/mL), and the cell death was confirmed using fluorescent microscopy.

Conclusion:
The antibacterial activity of these nanoparticles suggests that the synthesized nanoparticles can be used to treat bacterial infections of the skin.
Methodological Proposal for Strength and Power Training in Older Athletes:
A Narrative Review
Authors: Jofré-Saldía Emilio , Villalobos-Gorigoitía Álvaro , Gea-García Gemma 
Published: March 2022

Introduction:
Within the adult population, it is not uncommon to meet older athletes
who challenge the negative stereotypes associated with aging. Although their physical performance
is superior to their sedentary counterparts, they are not immune to impaired neuromuscular function,
leading to a decreased physical capacity and an increased risk of injuries. Despite the abundant
information about the benefits of strength/power training in advanced ages, there are no methodological
proposals that guide physical activity professionals to program this type of training.

Objective:
This study aimed to review the factors related to the decrease in sports performance
within older athletes and the benefits of a strength/power program in order to provide a methodological
proposal to organize training in this population.

Methods:
This is a review article. First, databases from PubMed, Science Direct, and SPORTSDiscus
and search engines, namely Google Scholar and Scielo, were reviewed, using standard keywords
such as strength and power training, evaluation of physical performance, neuromuscular
function, and risk of injury in the elderly athlete. All related articles published during the period
1963 to 2020 were considered. A total of 1837 documents were found. By removing 1715 unrelated
documents, 122 articles were included in the study after revision control.

Result:
Strength/power training is key to alleviating the loss of performance in older athletes and
the benefits in neuromuscular function, which helps reduce the rate of serious injuries, maintaining
sports practice for a longer period of time. In order to design an appropriate program, a prior evaluation
of the individual’s physical-technical level must be carried out, respecting the biologicalpedagogical
principles and safety regulations.

Conclusion:
The methodological proposal delivered in this review can serve as a technical guide
for physical activity professionals, which will be able to structure the strength/power training and
thus preserve the sports practice in older athletes for a longer time.
An mHealth Self-management System for Support Children With Acute Lymphocytic Leukemia and Their Caregivers: Qualitative Co-design Study
Authors: Mehdizadeh Hamed , Asadi Farkhondeh , Emami Hassan , Mehrvar Azim , Nazemi Eslam 
Published: March 2022

Background
The unique features of smartphones have extended their use in different fields, especially in the health care domain. These features offer new opportunities to support patients with chronic conditions by providing them with information, education, and self-management skills. We developed a digital self-management system to support children with cancer and their caregivers in Iran (low- and middle-income country).

Objective
This study is aimed at the development and preliminary evaluation of a cancer self-management system (CanSelfMan) tailored to the needs of children with cancer and their parents or caregivers.

Methods
This study was conducted in collaboration with a multidisciplinary team between January and February 2020 at MAHAK’s Pediatric Cancer Treatment and Research Center. We developed a self-management system in six stages: requirement analysis, conformity assessment, preparation of educational content, app prototyping, preliminary evaluation, and developing the final version.

Results
A total of 35 people (n=24, 69% parents and n=11, 31% children) volunteered to participate in the study. However, only 63% (15/24) of parents and 73% (8/11) of children were eligible to participate. By adopting a user-centered design approach, we developed a mobile app, CanSelfMan, that includes five main modules (knowledge base, self-management tips, self-assessment report, ask a question, and reminders) that provide access to reliable information about acute lymphocytic leukemia and the self-management skills required for side effect measurement and reporting. A web-based dashboard was also developed for oncologists and included a dashboard to monitor users’ symptoms and answer their questions.

Conclusions
The CanSelfMan app can support these groups by providing access to reliable information about cancer, facilitating communication between children or parents and health care providers, and helping promote medication adherence through a reminder function. The active participation of the target group can help identify their needs. Therefore, through the involvement of stakeholders such as patients, caregivers, and oncologists in the design process, we improved usability and ensured that the final product was useful. This app is now ready to proceed with feasibility studies.
Pemaksaan Perkawinan Berkedok Tradisi Budaya: Bagaimana Implementasi CEDAW terhadap Hukum Nasional dalam Melindungi Hak-Hak Perempuan dalam Perkawinan?
Authors: Fanny Nainggolan Junita , Ramlan Ramlan , Harahap Rahayu Repindowaty 
Published: February 2022

Artikel ini membahas mengenai implementasi CEDAW kedalam hukum nasional untuk melindungi hak-hak perempuan dalam perkawinan. Meski sudah puluhan tahun konvensi diratifikasi, namun dalam kehidupan bermasyarakat, perempuan masih sering didiskriminasi. Salah satu bentuk diskriminasi di Indonesia masih berupa budaya patriarki yang berkembang di masyarakat. Patriarki yang mendominasi budaya masyarakat menciptakan seksisme dan ketidaksetaraan gender yang mempengaruhi banyak aspek aktivitas manusia, salah satunya adalah masalah pernikahan. Di Indonesia, masih banyak perkawinan yang terjadi di Indonesia karena kawin paksa. Apalagi dilakukan dengan kedok tradisi yang telah mengalami pergeseran nilai budaya. Pilihan untuk menikah dan dengan siapa berkaitan erat dengan penentuan nasib sendiri yang telah diakui dalam Konvensi CEDAW. Hasil penelitian ini menunjukan bahwa di dalam Konvensi CEDAW, setiap orang memiliki hak yang sama untuk menikah, terlepas dari gender dan jenis kelamin orang tersebut. Namun, di dalam pengimplementasiannya ke dalam hukum nasional, pelaksanaannya masih bersifat diskiminatif dan belum terintegrasi.
RACIAL PROBLEMS IN ZADIE SMITHâ€™S 'ON BEAUTY'
Authors: Fahma Aqlia Nur , Basuki Imam , Wardani L. Dyah Purwita 
Published: February 2022

This paper discusses the problems of racism in the novel On Beauty. The analysis focuses on both individual and systemic racism represented in the novel as well as the critical position of the author. It employed Stuart Hall theory under qualitative method of analysis. The textual analysis was made to the narratives and dialogues in the novel which constitute racism in their meanings. It was concerned with how racism happens in the storyline. Meanwhile, the contextual analysis was carried out by using related references to supports the findings and the researchersâ€™ arguments. The result showed that black women still accepted Â negative stereotypes and became the objects of white peopleâ€™s oppressions. They were mistreated despite the application of the law on equal protection for women and minorities. In 2000s, systemic racism continued and made it even harder for black people to get the same treatment as the whites. They suffered from the policies, particularly educational policies which had led to the increase of the poverty rate of black people.
PENGARUH KARAKTERISTIK PERSONALITAS MANAJER TERHADAP HUBUNGAN ANTARA PARTISIPASI DALAM PENYUSUNAN ANGGARAN DENGAN KINERJA MANAJERIAL
Authors: Wirjono Endang Raino , Raharjono Agus Budi 
Published: February 2022

This research examined the effects of budget participation on performance of manager in firms. Prior studies in participative budgeting suggest that personality characteristic is potential ly important explanatory variables to motivate manager achieving good performance. Data were col lected from 85 managers of firms in Yogyakarta. Results of this study indicated that interaction between need of independence and participation had significant impact on managerial performance. This research also proved that interaction between need of authority and participation in setting budgeting had significant impact on managerial performance.Keywords: need of independence, need of authority, participative budgeting, managerial performance
SENSITIFITAS KINERJA PASAR MODAL INDONESIA DAN NILAI TUKAR RUPIAH TERHADAP PENYEBARAN KRISIS ASIA
Authors: Setyastuti Rini 
Published: February 2022

This paper tries to examine the impact of Asia crisis on Indonesian stock market and exchange rate. Despite Engle-Granger procedure and Johansen Criterion, error correction model (ECM) of the two variables are employed to simultaneously estimate the short run and the long run dynamics of the variables. Using monthly data covering the January 1995 to December 2006, empirical results showed that there is a cointegration between variables in the crises period but no cointegration before crises period. The result of ECM estimation proves there is a positive effect on the exchange rate when the Indonesian stock price increased and Thailand exchange rate appreciated. It is because the foreign investors enter the domestic stock market. In the crises period, we can prove that the depreciation of domestic currency and Thailand currency have a positive effect on the stock price in the long run. On the other hand, it is evident that there is a stable parameter of analysis.Keywords : Exchange Rate, Stock Price, Interest Rate, Error Correction Model