bluuebunny/crossref_metadata_2025_split huggingface dataset:
New dual-function in situ bone repair scaffolds promote osteogenesis and reduce infection
Authors: Changsheng Yang , Lei Zhou , Xiaodan Geng , Hui Zhang , Baolong Wang , Bin Ning 
Published: June 2022

Abstract
Background The treatment of infectious bone defects is a difficult problem to be solved in the clinic. In situ bone defect repair scaffolds with anti-infection and osteogenic abilities can effectively deal with infectious bone defects. In this study, an in situ polycaprolactone (PCL) scaffold containing ampicillin (Amp) and Mg microspheres was prepared by 3D printing technology.Results Mg and Amp were evenly distributed in PCL scaffolds and could be released slowly to the surrounding defect sites with the degradation of scaffolds. In vitro experiments demonstrated that the PCL scaffold containing Mg and Amp (PCL@Mg/Amp) demonstrated good cell adhesion and proliferation. The osteogenic genes collagen I (COL-I) and Runx2 were upregulated in cells grown on the PCL@Mg/Amp scaffold. The PCL@Mg/Amp scaffold also demonstrated excellent antibacterial ability against E. coli and S. aureus. In vivo experiments showed that the PCL@Mg/Amp scaffold had the strongest ability to promote tibial defect repair in rats compared with the other groups of scaffolds.Conclusions This kind of dual-function in situ bone repair scaffold with anti-infection and osteogenic abilities has good application prospects in the field of treating infectious bone defects.
Spotted leaf 11 (SPL11) contributes plant growth and adaptation to nitrogen limitation in rice
Authors: Thein Amy , Cao Yaqian , Shi Jia , Xue Mengyu , Wei Yiming , Yu Chao , Yang Fenghuan , Tian Fang , Chen Huamin 
Published: June 2022

Abstract
The ubiquitin-proteasome system (UPS) involves in many plant processes and responses to abiotic and biotic stresses. SPL11 (spotted leaf 11, SPL11) encoding a U-box E3 ligase negatively regulates flowering, plant defense and lesion-mimic phenotype. Our previous study discovered the up-regulation of SPL11 under limited nitrogen (LN) condition. However, it is little known that whether SPL11 mediates plant growth and adaptation to LN stress. Here, we further investigated the growth including height, biomass accumulation, nitrate content and NRT gene expression in spl11 mutant and SPL11 OX plants under HN and LN conditions. The spl11 mutant showed impaired growth compared with the wild type (WT) IR64 regardless of nitrogen supply conditions. SPL11 OX plants showed increasing growth compared with the WT TP309 only under HN but not LN condition. The expression of NRT genes supported the above phenotypes. In addition, under LN condition, the spl11 mutant showed earlier and severer senescence than the WT IR64, while no significant difference was found in the other three tested varieties. All these results suggested that SPL11 is essential to plant growth, adaption to LN stress, and repression of LN-caused early senescence. Moreover, the E3 ligase activity of SPL11 possibly inhibited by LN stress may also be required for the above regulatory functions. This study unveils novel regulatory functions of SPL11 on plant growth, adaption to LN stress and LN-caused early senescence.
METTL3 affects FLT3-ITD+ Acute Myeloid Leukemia by mediating autophagy by regulating PSMA3-AS1 stability
Authors: wu shenghao , Weng Shanshan , Zhou Wenjin , Chen Yuemiao , Liu Zhen 
Published: June 2022

Abstract
Objective
The study was designed to explore the role of PSMA3-AS1 in initiation and progression of acute myeloid leukemia (AML) and investigate its action mechanism.
Methods
Expression of PSMA3-AS1, miR-20a-5p and ATG16L1 both in vitro and in vivo was measured by qRT-PCR. The expression of protein was detected by western blot assay. Edu staining and flow cytometry were utilized to measure cell proliferation and apoptosis. Potential target was predicted by bioinformatics and was verified by dual-luciferase report gene assay and RNA pull down assay. QRT-PCR was used to quantify autophagy (LC3, Beclin1, P62) related genes. The m6A modification test is used to verify the effect of METTL3 on PSMA3-AS1. Tumor model was used to identify the effect of PSMA3-AS1 on tumor growth in vivo, and immunohistochemistry was applied to detect expression of ki67 and TUNEL.
Results
The results indicate that PSMA3-AS1 was upregulated in FLT3-ITD + AML patients. Si-PSMA3-AS1 can inhibit the proliferation, autophagy and inhibit the apotosis in MV4-11 cells. METTL3 could enhances the PSMA3-AS1 RNA stability. In addition, this study revealed that PSMA3-AS1 affected FLT3-ITD + AML by targeting expression of miR-20a-5p, and miR-20a-5p further modulated expression of ATG16L1, an mRNA that down-regulated in AML, to affect disease advancement.
Conclusion
PSMA3-AS1 could promote FLT3-ITD + AML progression by regulating the level of autophagy through the miR-20a-5p/ATG16L1 pathway. In addition, the increase of PSMA3-AS1 may be caused by the involvement of METTL3 in regulating its stability. This discovery will provide new horizons for early screening and targeted therapy of FLT3-ITD + AML.
Does Indian real estate regulation protect urban homebuyers? policy implications
Authors: Chawla Neelam , Kumar Basanta , Patel Gokulananda 
Published: June 2022

Abstract
With the passage of the Indian Real Estate (Regulation and Development) Act, 2016 (RERDA, 2016/Act), the previously unregulated urban housing and real estate market scenario began to change. According to the promoters' evasive and aggressive approach, the Act has saved homebuyers from exploitation and impacted society. The paper examines relevant statutory provisions and judicial decisions protecting urban homebuyers' interests. It examines their impact on them in particular and society as a whole. The study examines the reactions of 751 respondents comprising 540 homebuyers, 87 unsuccessful buyers, 59 builders, 56 officials, and nine experts using descriptive statistics, ANOVA, and a fuzzy analytical hierarchy process. The findings show that builders frequently take advantage of homebuyers. The builders' lobby and regulatory capture are on the horizon. Political willpower and strained interstate relations work with homebuyers. The findings demonstrate that timely judicial interventions have removed bottlenecks created by builders, benefiting both builders' and governments' efforts to protect homebuyers' interests. Nonetheless, even six years after its implementation, the Act's performance remains uneven. The study has research implications for the theory, policy, and socioeconomic practice in the real estate market.
Platinum Shortage Threatens China's Hydrogen Ambition for Carbon Neutrality
Authors: Wang Peng , Yang Fan , Wang Chenyang , Hubacek Klaus , Sun Laixiang , Chen Wei-Qiang , Feng Kuishuang , Li Jiashuo 
Published: June 2022

Abstract
Hydrogen is gaining tremendous traction in China as the fuel of the future to support the country’s carbon neutrality ambitions. The promising role of hydrogen for use as fuel is heavily reliant on platinum (Pt) and the interactions between hydrogen fuel industry and Pt supply constraints have been largely overlooked. In this research, we develop an integrated assessment model to explore China’s demand for hydrogen fuel and the associated Pt needs under different climate targets. The results indicate that China’s annual demand for hydrogen fuel would reach 20 million tons (mt) under the business-as-usual baseline scenario and 38 mt under the carbon-neutrality scenario until 2060, being 111–211 times the amount in 2020. This booming demand would lead China’s cumulative demand for Pt metal produced from virgin ore to reach 2,500-3,258 tons (cost of 86–112 billion USD), which is 38–50 times of China’s indigenous Pt reserve. Although reduction of Pt loading and enhanced recycling are identified as effective measures to alleviate the Pt supply constraint, their efforts alone are far from sufficient to fill the large demand-supply gap under both baseline and carbon-neutrality scenarios. Thus, joint efforts from global sustainable Pt sourcing as well as domestic material innovations are urgently needed so that China will be able to address Pt supply risks and achieve her hydrogen ambition towards carbon neutrality.
Genome analyses provide insights into epidermal pigmentation and adaptability to the hygrothermal environment in Youzhou dark goat (Capra Hircus)
Authors: Ren Hangxing , Wang Meng , Jiang Jing , Fu Lin , Li Jie , Sun Xiaoyan , Lin Yu , Mao Ke , Liu Liangjia , Chen Cancan , Zhang Li , Dong Xianwen , Zhao Jinhong , Li Nianfu , Jiang Wenkai , Li Ruiqiang , Wang Gaofu , Zhou Peng 
Published: June 2022

Abstract
Background
Youzhou dark goat is a rare indigenous goat breed that features its two outstanding characters including the dark skin with the white coat hair and the excellent adaptability to hygrothermal environment. Elucidation of the mechanisms of domestication and selection for these two traits on genome-wide level would greatly benefit medical and agricultural production. In the present study, we first de novo assembled and characterized the reference genome of the Youzhou dark goat on the Illumina HiSeqX platform. Then we identified its variation, selection signatures and the geographical events to reveal the phenotypic evolution and speciation in goat by comparative genomics and whole-genome resequencing methods. In addition, we also investigated the population structure and domestication of goat with different purpose of production in 11 populations of domestic goat.
Results
We achieved the reference genome in Youzhou dark goat (Contig N50 = 94 kb, size = 2.62Gb) and identified the gene families that contribute to their phenotypic characteristics. Comparative genome analysis illustrated that a single base mutation in MC1R gene (c.676A > G) and structural variation in ASIP gene are most likely the candidate causative genes for the phenotype of epidermal hyperpigmentation in Youzhou dark goat. Strong selection of PIK3R3, GNAS, and GNA11 genes suggests a coevolution of the melanogenesis and the resistance to parasite, which further contributes to the excellent adaptability to the hygrothermal environment for Youzhou dark goat. Population genetic analysis indicated that Youzhou dark goat was a relative ancient goat population and that the domestication of dairy trait occurred ahead of cashmere and meat trait in goat.
Conclusions
The Youzhou dark goat was a relative ancient goat population and the MC1R-ASIP signaling is most likely the candidate signaling pathway of epidermal pigmentation. Strong selection of PIK3R3, GNAS, and GNA11 genes play key roles in its excellent adaptability to the hygrothermal environment for Youzhou dark goat.
“I am alive because of her”: Factors Affecting Adherence to Combination Antiretroviral Therapy among People living with HIV in KwaZulu-Natal, South Africa
Authors: Loveday Marian , Furin Jennifer , Hlangu Sindisiwe , Naidoo Tasneem 
Published: June 2022

Abstract
Background: People living with HIV need to take lifelong, combination antiretroviral therapy (cART) for, but there have been only limited explorations of how factors affecting adherence can change over the course of an individual’s lifetime. Methods: We carried out a qualitative study of men and women living with HIV in KwaZulu, Natal, South Africa who were prescribed cART and who had periods of higher and lower adherence. Results: 18 individuals participated in open-ended interviews. Using a dynamic theory of adherence, we identified factual, relational, and experiential factors that were associated with adherence and non-adherence to cART. Periods of non-adherence were commonly reported. Participants described relationships and experiences as being important influences on their ability to adhere to cART throughout their treatment journeys. Conclusions: Periods of non-adherence to cART are common. While many cART counseling models are based on conveying facts to people prescribed cART, providing opportunities for supportive relationship where people can process their varied experiences is likely important to maintaining health for people living with HIV.
High-speed train derailment detection under complex track irregularities using a dynamic vehicle-track interaction model
Authors: Fei Yang , Jialin Sun , Zilong Wei , Zhou Yunlai , Wahab Magd Abdel 
Published: June 2022

Abstract
The track geometry is essential for the safety, stability and comfort of passing trains. Track irregularities, which have been extensively studied with specified single irregularity type, frequently exist in complex form, leading to potential derailment accidents especially in curved lines. This study investigates the dynamic vehicle-track interaction considering a series of complex irregularities. Furthermore, the safety indices indicating the possibility of derailment, e.g., derailment coefficient, wheel unloading rate and wheelset lateral force, are obtained for both C70 freight train track and 25 tons passenger train. The results indicate that the most dangerous situation is continuous multi-wave reverse complex irregularity of cross level and alignment on the high rail at the point of curve to spiral. We also found that the most unfavorable wavelength of complex irregularity is 10 m, and derailment risk of light freight train passing is obviously greater than that of passenger trains and heavy freight trains. Certain results can give further guideline for the condition monitoring and maintenance of the rail management.
Effects of Goal-oriented Prenatal Education on Birth Preparedness, Complication Readiness and Institutional Delivery Among Semi-urban Pregnant Women in Nigeria: a Quasi-experimental Study
Authors: Akinwaare Margaret Omowaleola , Oluwatosin Oyeninhun Abimbola 
Published: June 2022

Abstract
Background: Pregnancy and childbirth periods are critical in the lives of women, and Birth Preparedness and Complication Readiness (BPCR) has been proven to be effective in reducing maternal mortality globally. However, high maternal mortality, in Nigeria, is associated with inadequate BPCR and non-institutional delivery. This study evaluated the effects of Goal-Oriented Prenatal Education (GOPE) on pregnant women's BPCR and institutional delivery. Methods: The study adopted a quasi-experimental two-group pre and post-test design. Four hundred pregnant women were randomly selected from two out of six semi-urban Local Government Areas of Ibadan and were randomized into the Intervention group and Control group. John Hopkins Program for International Education in Gynaecology and Obstetrics questionnaire was adapted for the data collection on knowledge and attitude of BPCR at baseline, two weeks, and six weeks post-intervention. The pregnant women in the intervention group received GOPE focusing on knowledge and attitude of BPCR. A checklist was used for assessment of BPCR practice at twelve weeks post-intervention. The place of birth was documented at delivery. Data were analyzed using, descriptive statistics, and the Mann-Whitney U test at α0.05. Results: Good knowledge of BPCR was found in 65.5% of pregnant women at baseline and 91.8% post-intervention. A positive attitude to BPCR was found in 22.5% of pregnant women at baseline and 54.7% post-intervention. Good BPCR practice was found in 95.3% and 73.1% of women in the intervention and control groups respectively. At delivery, 93.5% and 53.5% had institutional delivery in the intervention and control groups respectively. A significant difference (p<0.001) was observed in BPCR knowledge and attitude post-intervention, as well as in BPCR practice and institutional delivery between women in the intervention and control group.Conclusions: Goal-oriented prenatal education improved birth preparedness and complication readiness as well as institutional delivery among pregnant women. This should be integrated into routine prenatal education in Nigeria.
An Evaluation of The Impact of Increased Lyme Disease Prevalence on New York State Public Health Department Action in the Face of Climate Change
Authors: Krevlin Zoe A. , Strong Aaron L. 
Published: June 2022

Abstract
Lyme disease is a zoonotic disease transmitted to humans from black-legged ticks (Ixodes scapularis) carrying the bacterium Borrelia burgdorferi that can cause serious complications if not detected and treated in a timely manner. While Lyme disease has only been present in the United States since 1970, it has quickly become endemic to the northeast United States. And since 1990, caseloads have dramatically risen and are estimated to be close to 400,000 cases a year. The increase in incidence rates is attributable to climate change. As the planet warms, the number of tick vectors increases, vector mortality rates lessen, vector life cycles become shorter, and vector active seasons become longer. In New York State there has been a northward expansion of ticks and Lyme disease cases from hyperendemic areas (like the Hudson Valley) to emerging areas (like the Adirondacks.) Public health action has the potential to halt the spread of Lyme disease into areas where incidence is currently low. This study aims to evaluate barriers to New York State county-level public health departments taking action to prevent and combat the spread of Lyme disease. Through a confidential survey, it was determined that financial and human resources are consistently the greatest barriers to county-level Lyme disease prevention activities. Data availability was found to be significantly more of a barrier in counties where Lyme disease is still emerging than in counties where Lyme disease is already hyperendemic. To prevent counties where Lyme disease is emerging from becoming hyperendemic, it is imperative to establish robust and accurate data surveillance systems. Appropriate data systems would also be essential in preventing the spread of other tick-borne diseases in the face of climate change, such as anaplasmosis and babesiosis in New York State.
Anatomic variations of the hepatic arterial irrigation in a Mexican population: CT evaluation, a cross-sectional study
Authors: Castillo-Martínez Daniel B. , Palacios-Rivera D. Michelle , Serratos Jorge Boy 
Published: June 2022

Abstract
Introduction: Knowledge and mastery of the anatomical variability of the hepatic arteries are essential for surgical disciplines and, given their complexity and importance over time, they have been extensively studied and described.Material and method: A retrospective, cross-sectional, analytical study was carried out in which contrasted tomographic studies carried out during 2021 at the North Central PEMEX Hospital were analyzed. Statistical analysis was performed with the statistical software IBM® SPSS© Statistics.Results: 207 CT scans were analyzed, and Uflacker type I CT was found in 90.8%, the Michel class I hepatic arterial irrigation in 70.5%, and the origin of CA was documented in the RHA in 90.3%. Multiple CAs were identified in 1% and Moynihan's hump in 0.5%. The mean length of the CHA was 53 millimeters.Conclusions: The study by tomography of the anatomical variants of hepatic irrigation is an accessible and non-invasive tool. The nomenclature described in the present work allows a precise and straightforward understanding of clinical-surgical utility.
Relationship between human cytomegalovirus glycoprotein B genotypic distributions, virus load and demographic and clinical features in a Chinese infant population
Authors: Mu Huijun , Qiao Weizhen , Zou Jian , Zhang Haiping 
Published: June 2022

Abstract
Purpose
To explore the relationship between human cytomegalovirus (HCMV) glycoprotein B (gB) genotype distribution, virus load and demographic and clinical features in Chinese infants.
Methods
The retrospective data of 265 infants who underwent urine HCMV DNA test were analyzed. The viral load and gB genotype were further detected in 91 HCMV positive infants by quantitative fluorescence polymerase chain reaction (PCR) and DNA sequencing, respectively.
Results
The positive rate of HCMV infection was 46.04% (122/265), and it reached the peak at the age of two months (9.62%, 25.81% and 68.57% in infants aged 0–30 days, one month and two months, respectively). As for the 91 infants studied furtherly, the top two clinical diagnoses are hepatitis (34/91, 37.36%) and lower respiratory tract infection (21/91, 23.07%). The most prevalent genotype was gB3 (47/91, 51.65%), followed by gB1 (44/91, 48.35%). The gB3 HCMV infection was more prevalent in infants aged 0–2 months (gB1 = 14, gB3 = 22) than in other infants aged 3–12 months (gB1 = 20, gB3 = 11, χ2 = 4.38, p = 0.0364). The mean level of HCMV DNA was 1.047×104 copies/ml. There was no significant difference in viral load among infants infected with gB1 or gB3 genotype HCMV. The data also showed that the ALT and AST levels in the anti-HCMV IgM + and IgG– group are significantly higher than those in the anti-HCMV IgM + and IgG+, and IgM- and IgG + group.
Conclusions
This study revealed the infection status and process of HCMV in Chinese infants. In addition, this study suggests that anti-HCMV IgG may play a role in protecting liver function.
Grey Wolf Optimizer-based decentralized service discovery in Internet of Things applications
Authors: AZIZOU Zahia BENSALAH , BOUDRIES Abdelmalek , AMAD Mourad 
Published: June 2022

Abstract
Discovery of service is one of the main challenge in Internet of Things due to the increasing of services and applications, which is especially tricky for the lookup of service (or set of services). Therefore , in this paper , we propose a new distributed discovering algorithm based on Grey Wolf Optimizer ( GWO ) for IoT . GWO is one of recent metaheuristic , the field of swarm intelligence to solve combinatorial optimization problem. To show the different effective performances of GWO in accurate discovering of services, The simulation results show that GWO can achieve a high discovery success with small number of hops used to discover service. Our approach maintains their performance and achieve good scalability as the number of object in decentralized approach for IoT .
A Privacy Preserving E-Voting System with Two-Phase Verification based on Ethereum Blockchain
Authors: Goyal Jitendra , Ahmed Mushtaq , Gopalani Dinesh 
Published: June 2022

Abstract
In recent years, With the rapid development of blockchain technol- ogy, there has been an increased interest in solving various opera- tional challenges. For the e-voting system at present, voter’s privacy, immutability of votes and third party dependency are major issues. These issues can very well be solved by blockchain technology. In this paper, we have proposed a decentralized framework for e-voting based on blockchain technology that contributes significantly to the implementation paradigm. We have implemented it on the Ethereum platform and developed a fully functional smart contract to store and validate votes. The research provides deep insight into the design of smart contract transactions in an e-voting system from a computa- tional cost perspective. In order to authenticate the voters, We have done voter verification in two phases. In the first phase, the voter reg- isters through a secure code, whose(code) Keccak-256 hash is stored on the blockchain, and in the second phase, the voter is verified by the managing authority. Based on the literature review, we found a lack of standardization for blockchain-based e-voting systems. So we have tried to fill this gap of standardization. In addition, we have also inspectedour model against various security attacks and found that our model is robust against double-spend attack, re-entrancy attack, DDoS etc.