bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the role of transcription factor SPI1 in the development of glioma
Authors: Du Baoshun , Gao Wuji , Qin Yu , Zhong Jiateng , Zhang Zheying 
Published: April 2022

Abstract
Background
Glioma is a common malignant brain tumor. The purpose of this study was to investigate the role of the transcription factor SPI1 in glioma.

Methods
SPI1 expression in glioma was identified using qRT-PCR and Western blotting. Cell proliferation was assessed using the CCK8 assay. Transwell and wound healing assays were utilized to evaluate cell migration. Additionally, cell cycle and apoptosis were detected using flow cytometry.

Results
We observed that the expression level of SPI1 was up-regulated in glioma tissues, compared to normal tissues. Furthermore, we found that SPI1 is able to promote proliferation and migration of glioma cells in vitro. Flow cytometry results demonstrate that, compared to si-NC cells, si-SPI1 cells stagnated in the G1 phase, and down-regulation of SPI1 expression is able to increase rates of apoptosis. Double luciferase activity and chromatin immunoprecipitation assay results indicated that SPI1 can bind to the promoter sites and promote the proliferation and migration of glioma cells by regulating the expression of oncogenic PAICS.

Conclusions
Our results suggest that SPI1 can promote proliferation and migration of glioma. Furthermore, SPI1 can be utilized as a potential diagnostic marker and therapeutic target for glioma.
That person is now with or without a mask: how encoding context modulates identity recognition
Authors: Garcia-Marques Teresa , Oliveira Manuel , Nunes Ludmila 
Published: April 2022

AbstractPrevious research has mostly approached face recognition and target identification by focusing on face perception mechanisms, but memory mechanisms also appear to play a role. Here, we examined how the presence of a mask interferes with the memory mechanisms involved in face recognition, focusing on the dynamic interplay between encoding and recognition processes. We approach two known memory effects: (a) matching study and test conditions effects (i.e., by presenting masked and/or unmasked faces) and (b) testing expectation effects (i.e., knowing in advance that a mask could be put on or taken off). Across three experiments using a yes/no recognition paradigm, the presence of a mask was orthogonally manipulated at the study and the test phases. All data showed no evidence of matching effects. In Experiment 1, the presence of masks either at study or test impaired the correct identification of a target. But in Experiments 2 and 3, in which the presence of masks at study or test was manipulated within participants, only masks presented at test-only impaired face identification. In these conditions, test expectations led participants to use similar encoding strategies to process masked and unmasked faces. Across all studies, participants were more liberal (i.e., used a more lenient criterion) when identifying masked faces presented at the test. We discuss these results and propose that to better understand how people may identify a face wearing a mask, researchers should take into account that memory is an active process of discrimination, in which expectations regarding test conditions may induce an encoding strategy that enables overcoming perceptual deficits.
Incorporating patient experience into drug development for ulcerative colitis: development of the Urgency Numeric Rating Scale, a patient-reported outcome measure to assess bowel urgency in adults
Authors: Dubinsky Marla C. , Irving Peter M. , Panaccione Remo , Naegeli April N. , Potts-Bleakman Alison , Arora Vipin , Shan Mingyang , Travis Simon 
Published: April 2022

Abstract
Background
Bowel urgency, the sudden or immediate need to have a bowel movement, is a common, bothersome and disruptive symptom of ulcerative colitis (UC). UC treatment goals include control of urgency; however, it is not consistently assessed in UC clinical trials. The Urgency Numeric Rating Scale (NRS) is a new patient-reported measure to assess severity of bowel urgency in adults with UC developed in accordance with Food and Drug Administration guidelines.

Methods
Qualitative interviews were used to develop Urgency NRS. The scale asks patients to report the immediacy status of their UC symptom over the past 24 h on an 11-point horizontal numeric rating scale [0 (No urgency) to 10 (Worst possible urgency)]. Higher scores indicate worse urgency severity. A 2-week diary study assessed floor and ceiling effects, test–retest reliability (intraclass correlation coefficient (ICC) (2,1) between Week 1 and 2), and construct validity (Spearman correlation using Week 1 scores). Weekly scores were calculated as mean score over each 7-day period.

Results
Qualitative interviews with 16 UC patients (mean age 37.9 ± 11.6 years; 50% female; 56% White) confirmed relevance, content, and comprehensiveness. The 2-week diary study included 41 UC patients (mean age 44.2 ± 14.6 years; 51% female; 56% White). No ceiling or floor effects were identified. Test–retest reliability was high (ICC = 0.877). Average Urgency NRS and patient global rating of severity scores were highly correlated, with a moderate correlation between average Urgency NRS and stool frequency, demonstrating construct validity.

Conclusions
Bowel urgency is a distinct symptom of UC. The Urgency NRS is a well-defined, content-valid, and reliable measurement of bowel urgency in adults with UC.
Dermatomyositis post vaccine against SARS-COV2
Authors: Camargo Coronel Adolfo , Jiménez Balderas Francisco Javier , Quiñones Moya Horacio , Hernández Zavala Mario Raúl , Mandinabeitia Rodríguez Pedro , Hernández Vázquez José Ramiro , Zamora Zarco Sandy , Aguilar Castillo Sergio De Jesús 
Published: April 2022

Abstract
Background
Dermatomyositis belongs to an infrequent group of diseases predominantly found in patients older than 40 years old and is characterized by dermal and muscular findings. This disease presents itself as proximal, ascending and symmetric weakness and typical dermatosis with findings such as elevated muscle enzymes, altered electromyography and typical changes in muscle biopsy; as of today, the etiology of the disease in unknown. The COVID-19 vaccine has been a fundamental tactic to achieve control of the coronavirus (SARS CoV2), and it’s clear that the benefits of getting the vaccine overweight the risks that might come along with it. Although rare, all adverse effects should be reported, this could help us to understand the elusive pathophysiology of inflammatory idiopathic myopathy.

Case presentation
In this text we will describe the case of a patient with dermatomyositis who was vaccinated against SARS CoV2 with BNT162b2 mRNA (Pfizer-BioNTech), showing a temporal relation between the vaccination and the beginning of her symptoms. We realized all the diagnostic approach to the suspected disease including electromyography, muscle biopsy and laboratory findings, corroborating the diagnosis. The patient received standard treatment for this disease (steroid therapy) and have a classic slow improvement.

Conclusions
Although it´s not possible to confirm a direct correlation between the vaccine and the onset of the disease, we considered that there are enough data to suspect that this could be a trigger event and therefore should always be considered a possible cause for a case of inflammatory idiopathic myopathy.
Assessment of the impact of green synthesized copper nanoparticles on freshwater snails (Indoplanorbis exustus) in comparison with field-control and lab-control snails
Authors: Mehraj Ahtisham , Pandit Sangeeta V. 
Published: April 2022

Abstract
Background
Nanomaterials are used extensively because of their optical, electrical, catalytic, and mechanical properties. To understand the biological processes in an experimental organism, we aimed to highlight the comparative results obtained in the field-control, lab-control, and experimental freshwater snails (Indoplanorbis exustus). Terminalia arjuna bark extract was used for the synthesis of CuNPs and for characterization SEM, XRD, and FTIR techniques were used.

Results
The estimated LC50 concentration for 96 h was to be 9.97 mg/L, thereafter animals were exposed further for 14 days to sub-lethal concentrations: 1/7th (1.42 mg/L), 1/5th (1.99 mg/L), 1/3rd (3.32 mg/L), and 1/2nd (4.98 mg/L). The data collected after conducting experiments on snails in the laboratory varied from the field-control snails. For comparison, the protein profile of field-control, lab-control, and experimental animals were displayed using sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then compared based on molecular masses. The bacterial isolates obtained were all belonging to streptococcus family. All isolates were Gram-positive and catalase-negative. A significant increase in bioaccumulation of CuNPs and dose-dependent necrosis was observed after 14 days.

Conclusions
The results obtained from the field control animals were different from those obtained in the lab control and experimental groups upon exposure to increasing concentration of CuNPs for 14 days.
The effects of OSAS corrective surgery and anesthesia on inflammatory and oxidative stress parameters in patients with obstructive sleep apnea syndrome
Authors: Ince Ulku , Kabukcu Hanife Karakaya , Bostanci Aslı , Ozdem Sebahat , Turhan Murat , Kucukcetin Ikbal Ozen 
Published: April 2022

Abstract
Introduction
Oxidative stress and airway inflammation are important in the pathophysiology of obstructive sleep apnea syndrome (OSAS).
We examined the levels of inflammation and oxidative stress parameters in patients without OSAS and grouped as mild, moderate, and severe OSAS according to the apnea-hypopnea index (AHI). In addition, we investigated the changes in these parameters in patients whose airway obstruction was resolved by surgical treatment.

Materials and method
The groups included 18 patients with an AHI below 5 (group 1), 28 patients with an AHI between 5 and 15 (group 2), 25 patients with an AHI between 15 and 30 (group 3), and 30 patients with an AHI of over 30 (group 4).
Blood samples were collected from patients following the induction of anesthesia (1st measurement), after the operation (2nd measurement), on the postoperative 3rd day (3rd measurement), and at the postoperative 2nd month (4th measurement). Arylesterase (ARE), paraoxonase (PON), nitrotyrosine (NT), leukocyte, CRP, and HDL were measured.

Results
The inter-group comparisons revealed differences in the 3rd measurement of leukocyte count and CRP value, in the 3rd and 4th measurements of HDL (p < 0.05). No significant difference was observed in the inter-group or intra-group comparisons for ARE, PON, and NT values (p > 0.05).

Conclusion
We observed that CRP, HDL PON, ARE, NT levels, and the leukocyte count were not related to the severity of OSAS in patients with OSAS. The difference observed in CRP and leukocyte count may be due to the continuous effect of the inflammatory effect of surgery in the early post-operative period. We thought that the increase in HDL in all groups after the 5th postoperative day was due to the surgical correction of airway obstruction. As a result, we concluded that CRP, HDL, PON, ARE, NT, and leukocyte levels are not markers for OSAS.
Young adults’ familiarity with different cannabis product terms: the need for standardized cannabis surveys
Authors: Berberian S. , Broussard M. L. , Tully C. , Methuku V. , Pardini D. A. , Meier M. H. 
Published: April 2022

Abstract
Background
Cannabis legalization has resulted in the proliferation of cannabis products. Participants’ familiarity with terms for these products may have implications for assessment, as unfamiliarity with particular terms may result in under-reports of use.

Methods
A convenience sample of 861 college students from one U.S. university completed a survey in the spring of 2020 about their familiarity with a variety of cannabis product terms and use of a variety of cannabis products.

Results
Participants varied in their familiarity with cannabis product terms. For example, with regard to terms for cannabis concentrates with very high concentrations of THC, 85% of participants reported being familiar with the term “wax pen or THC oil,” but only 27% reported being familiar with the term “butane hash oil (BHO)” (i.e., the oil that composes most concentrates). Moreover, of participants who reported use of concentrates based on selecting pictures of the products they had used (n = 324, 40%), 99% (n = 322) reported having seen a “wax pen or THC oil” based on a written list of product terms, whereas only 20% (n = 65) reported having seen “butane hash oil (BHO).” This suggests that asking about use of “butane hash oil” use may result in lower rates of cannabis concentrate use than asking about use of “wax pen/THC oil.” With regard to terms for marijuana flower, 29% of participants (n = 248) reported being unfamiliar with the term marijuana “buds or flowers.” Of participants who reported use of marijuana flower based on selecting pictures of the products they had used (38% of the sample, n = 329), only 86% (n = 282) reported having seen marijuana “buds or flowers” based on a written list of product terms. This suggests that asking about use of marijuana “buds or flowers” use could result in under-reporting due to lack of familiarity with that term. Finally, when asked to select pictures of the cannabis product(s) that participants thought constituted “marijuana,” participants most commonly selected pictures of marijuana flower (93%), followed by wax pen/THC oil (57%) and edibles (49%).

Conclusions
Young adults vary in their familiarity with cannabis product terms, and some may under-report cannabis use in surveys that rely on written cannabis product terms. Young adults also differ in terms of which cannabis products they think constitute “marijuana.” Although participants’ familiarity with specific cannabis product terms in this sample may not generalize to other populations, results highlight the need for standardized surveys of cannabis use that incorporate pictures of cannabis products to overcome issues related to variability in familiarity with cannabis product terms.
Fibromyalgia syndrome and dental anxiety
Authors: Ilhanli Manolya , Karacesme Mehmet Alptekin , Ilhanli Ilker , Celenk Peruze 
Published: April 2022

Abstract
Background
Fibromyalgia syndrome (FM) is often accompanied by anxiety. Dental anxiety is common in society and negatively affects the application of patients to the dentist.

Aims
We aimed to examine the presence of dental anxiety in FM patients and to compare it with the non-FM controls. The aim was also extended to examine the relationship between FM disease severity and dental anxiety.

Methods
Sixty-three patients diagnosed with FM and 50 age- and sex-matched non-FM controls were included. All participants were asked to answer the Modified dental anxiety scale (MDAS) and the Hospital Anxiety and Depression Scale (HAD anxiety and HAD depression, respectively). FM patients were also asked to answer the Fibromyalgia impact questionnaire (FIQ).

Results
There were 18 (28.57%) participants in the FM group with an MDAS score of ≥ 16 (anxiety), and 3 (6%) participants in the control group. All 10 participants with MDAS score ≥ 19 (dental phobic) were in the FM group (15.87%). There was a moderate positive correlation between FIQ score and MDAS Score. There were significant positive correlations between the MDAS Score and HAD anxiety and HAD depression scores.

Conclusions
The frequency of dental anxiety may be high in FM patients and disease activity may negatively affect dental anxiety. This is the first study to examine the presence of dental anxiety in fibromyalgia syndrome and its relationship with disease severity. This study draws attention to the presence of dental anxiety, which will negatively affect dental interventions and follow-ups in Fibromyalgia, which is quite common.
Evaluation of essential oils nanoemulsions formulations on Botrytis cinerea growth, pathology and grey mould incidence on cucumber fruits
Authors: Ziedan El-Sayed H. E. , Saad Moataza M. , El-Kafrawy Ahmed A. , Sahab Ahmed F. , Mossa Abdel-Tawab H. 
Published: April 2022

Abstract
Background
Grey mould caused by Botrytis cinerea of cucumber immature fruits is the most epidemic fungal diseases causing significantly losses of fruits during development on cucumber plant in winter season.

Material and methods
Crude and nanoemulsions of clove, black seed, lemon and orange essential oils at (5000 ppm) and also different formulations of clove, black seed and lemon oils nanoemulsions as single or in combination were tested against mycelial linear growth of B. cinerea (MF996363) as well sclerotial formation and pathological activity on the development of germinated seeds of cucumber.

Results
Essential oils nanoemulsion formulation of clove + black seed (2:1) at 5000 ppm was the best formulation significantly reduced mycelia linear growth of B. cinerea by 61.0% followed by 28% formulation (1:1) and (1:2). In addition, nanoemulsions formulations of clove, black seed essential oils (2:1) were highly reduced the count of sclerotial formation of B. cinerea and completely (100%) suppress seed rot and seedling mortality of cucumber followed by nanoemulsion of clove only.

Conclusions
Foliar application of nanoemulsion formulation of clove + black seeds is promising than fungicides (Topsin M-70) in controlling grey mould on cucumber fruits caused by B. cinerea in plastic greenhouse with no phytotoxicity on cucumber plants.
Phytochemical analysis of some selected traditional medicinal plants in Ethiopia
Authors: Agidew Misganaw Gedlu 
Published: April 2022

AbstractBackgroundThis review of relevant medicinal plants is based on the fundamental knowledge accumulated by indigenous people of Ethiopia and to identify which types of selected medicinal plants for phytochemical analysis were analyzed and which one is not analyzed at Ethiopian levels. In this review, the most traditional medicinal plant species found and used in Ethiopia are chosen.ResultsThe qualitative phytochemical analysis, some of which are the most important phytochemicals such as phenolic, tannins, alkaloids, saponins, cardiac glycosides, steroids, terpenoids, flavonoids, phlobatannins, anthraquinones, and reducing sugars are studied by the researcher. Most studies have revealed that some phytochemicals are present in some medicinal plants while some are absent. The phytochemical properties of some species were studied likeArtemisia afra(Ariti),Aloe Vera(Erret),Yzygium guineense(Dokuma),Ruta chalepensis(Tenadam),Ocimum grattissimum(Damakese),Nigella sativa(Tikur Azmud),Lepidium sativum(Feto),Hagenia abyssinica(Kosso),Croton macrostachyus(Bisana), andRhamnus prinoides(Gesho).ConclusionsThis review has shown that traditional medicinal plants whose phytochemical properties are not studied have various medicinal purposes like treating mastitis, preventing boils, hemorrhoids, congestion, headache, hepatitis, liver, vertigo, stomatitis, kidneys, liver, and vision for treating anemia, hemorrhoid coughs, fluxes, and stomatitis in most animals and human beings. So that identifying the plants based on the investigation and analysis of phytochemical properties of such plant species are more important than Ethiopian levels.
Husband’s influence in the use of long-acting reversible contraception (LARC) method in Special Region of Yogyakarta, Indonesia
Authors: Utami Fitriana Putri , Gustina Erni , Sulistiawan Dedik , Matahari Ratu , Sari Bakti Permata 
Published: April 2022

Abstract
Background
The long-acting reversible contraceptive method is one way to overcome the high population growth rate. The long-term contraception method is more efficient to regulate pregnancy because it can be used for a long period of time and is safer and more effective and the failure rate is relatively low at 0.2 per 100 users. The strong patriarchal culture in the Special Region of Yogyakarta is one of the factors that determine the wife's decision to use the long-term contraception method. Therefore, this study aims to determine the effect of knowledge, attitudes, and support from husbands in the use of the LARC method in the Special Region of Yogyakarta.

Method
This study used secondary data from the 2017 Indonesia Demographic and Health Survey (IDHS) taking the Special Region of Yogyakarta province as a level of analysis or as a place of research. The population was all fertile couples aged 15–49 years as many as 123 couples. The sample was as many as 71 respondents who had made the inclusion criteria refer to criteria used in the 2017 IDHS, namely fertile age couple who used LARC and non-LARC contraception. Data were analyzed by multivariate analysis.

Results
The results showed that there is no relationship between husband's knowledge (p value = 0.330), husband's attitude (p value = 1.000), and husband's support (p value = 0.771) on the choice of LARC in the Special Region of Yogyakarta.

Conclusion
Even though in a patriarchal culture, husbands do not play a major role in contraceptive choices because of the presence of women empowerment and misinformation about the contraceptive method itself, close communication between husband and wife and the socialization of LARC by health workers is expected to increase its use.
Official Position of the Brazilian Association of Bone Assessment and Metabolism (ABRASSO) on the evaluation of body composition by densitometry—part II (clinical aspects): interpretation, reporting, and special situations
Authors: Maeda Sergio Setsuo , Albergaria Ben-Hur , Szejnfeld Vera Lúcia , Lazaretti-Castro Marise , Arantes Henrique Pierotti , Ushida Marcela , Domiciano Diogo Souza , Pereira Rosa Maria Rodrigues , Marin-Mio Rosângela Villa , de Oliveira Mônica Longo , de Mendonça Laura Maria Carvalho , do Prado Mirley , de Souza Guilherme Cardenaz , Palchetti Cecília Zanin , Sarni Roseli Oselka Saccardo , Terreri Maria Teresa , de Castro Luiz Claudio Gonçalves , Artoni Silvana Martinez Baraldi , Amoroso Lizandra , Karcher Débora Emy , Prado Carla M. , Gonzalez Maria Cristina , de Medeiros Pinheiro Marcelo 
Published: April 2022

Abstract
Objective
To present an updated and evidence-based guideline for the use of dual-energy x-ray absorptiometry (DXA) to assess body composition in clinical practice.

Materials and methods
This Official Position was developed by the Scientific Committee of the Brazilian Association of Bone Assessment and Metabolism (Associação Brasileira de Avaliação Óssea e Osteometabolismo, ABRASSO) and experts in the field who were invited to contribute to the preparation of this document. The authors searched current databases for relevant publications in the area of body composition assessment. In this second part of the Official Position, the authors discuss the interpretation and reporting of body composition parameters assessed by DXA and the use of DXA for body composition evaluation in special situations, including evaluation of children, persons with HIV, and animals.

Conclusion
This document offers recommendations for the use of DXA in body composition evaluation, including indications, interpretation, and applications, to serve as a guiding tool in clinical practice and research for health care professionals in Brazil.