bluuebunny/crossref_metadata_2025_split huggingface dataset:
Persistent Musculoskeletal Deficits in Pediatric, Adolescent and Young Adult Survivors of Allogeneic Hematopoietic Stem-Cell Transplantation
Authors: Kindler Joseph M. , Guo Michelle , Baker Joshua , McCormack Shana , Armenian Saro H. , Zemel Babette S. , Leonard Mary B. , Mostoufi-Moab Sogol 
Published: January 2022

ABSTRACT
Allogeneic hematopoietic stem cell transplantation (alloHSCT) is a common therapy for pediatric hematologic malignancies. With improved supportive care, addressing treatment-related late effects is at the forefront of survivor long-term health and quality of life. We previously demonstrated that alloHSCT survivors had increased adiposity, decreased lean mass, and lower bone density and strength, 7 years (median) from alloHSCT compared to their healthy peers. Yet it is unknown whether these deficits persist. Our longitudinal study characterized changes in muscle and bone over a period of 3.4 (range, 2.0 to 4.9) years in 47 childhood alloHSCT survivors, age 5–26 years at baseline (34% female). Tibia cortical bone geometry and volumetric density and lower leg muscle cross-sectional area (MCSA) were assessed via peripheral quantitative computed tomography (pQCT). Anthropometric and pQCT measurements were converted to age, sex, and ancestry-specific standard deviation scores, adjusted for leg length. Muscle-specific force was assessed as strength relative to MCSA adjusted for leg length (strength Z-score). Measurements were compared to a healthy reference cohort (n = 921), age 5–30 years (52% female). At baseline and follow-up, alloHSCT survivors demonstrated lower height Z-scores, weight Z-scores, and leg length Z-scores compared to the healthy reference cohort. Deficits in MCSA, trabecular volumetric bone density, and cortical bone size and estimated strength (section modulus) were evident in survivors (all p &lt; 0.05). Between the two study time points, anthropometric, muscle, and bone Z-scores did not change significantly in alloHSCT survivors. Approximately 15% and 17% of alloHSCT survivors had MCSA and section modulus Z-score &lt; −2.0, at baseline and follow-up, respectively. Furthermore, those with a history of total body irradiation compared to those without demonstrated lower MCSA at follow-up. The persistent muscle and bone deficits in pediatric alloHSCT survivors support the need for strategies to improve bone and muscle health in this at-risk population. © 2022 American Society for Bone and Mineral Research (ASBMR).
Effect of Mg2+ additives on Nieuwland catalyst: The role of the second metal ionic radius
Authors: Zheng Ling , Lin Ruolin , Luo Dingjie , Guo Liang , Zhang Jinli 
Published: January 2022

AbstractIn the production of chloroprene rubber, acetylene dimerization over the Nieuwland catalyst is an important step. The addition of Mg2+ to the Nieuwland catalyst improves the acetylene conversion and the selectivity toward vinyl acetylene. In this study, active component crystal structure, the role of Mg2+ in the Nieuwland catalyst, the reason for the catalyst deactivation, and the reaction mechanism were analyzed by different characterization techniques. The results indicate that the main reason for the Nieuwland catalyst deactivation is the loss of Cu2+. The addition of Mg2+ will increase the exposure of active sites, reduce the residence time of acetylene in the catalyst, and inhibit the reduction of Cu2+, thereby improving the catalytic performance and stability of the catalyst.
Influence of diabetes and hypercholesterolemia on laboratory methods for hereditary spherocytosis diagnosis
Authors: Lazarova Elena , Gulbis Béatrice 
Published: January 2022

AbstractIntroductionHereditary spherocytosis (HS) is characterized by decreased erythrocyte deformability resulting in hemolytic anemia. This is a heterogeneous disease regarding underlying protein deficiency, disease severity, age at diagnosis and clinical course. Although largely considered as pediatric disease, HS could be initially diagnosed also in elder patients as a result of gallstones or splenomegaly fortuitous finding. Concurrently, common adulthood metabolic disorders like diabetes or dyslipidemia are also known to impair RBC rheology and deformability. Therefore, we aimed to investigate if these diseases affect the screening and diagnostic tools used for HS diagnosis.MethodsWe applied our workflow for HS diagnosis on 95 pathological samples: 29 patients with diabetes, 20 with hypercholesterolemia, 17 with dyslipidemia, 6 with hypertriglyceridemia, 23 with metabolic syndrome (MS). Thus, a total of 73 samples were analyzed by automated reticulocyte analysis, 52 by cryohemolysis test, and 41 by ektacytometry osmoscan analysis as we used two out of the three tests for each individual sample.ResultsApplying our screening algorithm based on automated reticulocyte indices, a total of 4 samples (4.2%): one sample (5%) from the diabetes group and three samples (16.7%) from the MS group, positioned into the HS zone. However, no significant difference was found between any of the pathological groups and the controls for the cryohemolysis test or the osmoscan.ConclusionWhile diabetes and hypercholesterolemia are pathologic conditions known to present with decreased erythrocyte deformability and disturbed rheology, their possible concomitant presence with HS would not interfere with the screening and confirmatory laboratory methods.
Homozygous missense variant in POPDC3 causes recessive limb‐girdle muscular dystrophy type 26
Authors: Ullah Anwar , Lin Zhaohan , Younus Muhammad , Shafiq Sarfraz , Khan Shazia , Rasheed Memoona , Mahmood Arif , Alqosaibi Amany I. , Alshehri Mohammed Ali , Khan Amjad , Umair Muhammad 
Published: January 2022

AbstractBackgroundLimb‐girdle muscular dystrophy (LGMD) comprises a heterogeneous group of diseases, affecting different muscles, predominantly skeletal muscles and cardiac muscles of the body. LGMD is classified into two main subtypes A and B, which are further subclassified into eight dominant and thirty recessive subtypes. Three genes, namely POPDC1, POPDC2 and POPDC3, encode popeye domain‐containing protein (POPDC), and the variants of POPDC1 and POPDC3 genes have been associated with LGMD.MethodsIn the present study, we performed whole‐exome sequencing (WES) analysis on a single‐family to investigate the hallmark features of LGMD. The results of WES were further confirmed by Sanger sequencing and 3D protein modeling was also conducted.ResultsWES data analysis and Sanger sequencing revealed a homozygous missense variant (c.460A>G; p.Lys154Glu) at a highly conserved amino acid position in the POPDC3. Mutations in the POPDC3 gene have been previously associated with recessive limb‐girdle muscular dystrophy type 26. 3D protein modeling further suggested that the identified variant might affect the POPDC3 structure and proper function.ConclusionsThe present study confirms the role of POPDC3 in LGMD, and will facilitate genetic counseling of the family to mitigate the risks of the carrier or affects on future pregnancies.
IL-27 signaling negatively regulates FcɛRI-mediated mast cell activation and allergic response
Authors: Wan Xiaopeng , Zhang Yuanyuan , Tang Huanna , Li Mengyao , Jiang Tianqi , He Jia , Bao Chunjing , Wang Junkai , Song Yinjing , Xiao Peng , Liu Yang , Lai Lihua , Wang Qingqing 
Published: January 2022

AbstractIL-27 is a member of the IL-12 family, exerting both anti- and pro-inflammatory activity in a cell-dependent and disease context-specific manner. Antigen-mediated cross-linking of IgE on mast cells triggers a signaling cascade that results in mast cell degranulation and proinflammatory cytokine production, which are key effectors in allergic reactions. Here, we show that the activation of mast cells is negatively regulated by IL-27 signaling. We found that mice lacking IL-27Rα (WSX-1) displayed increased sensitivity to IgE-mediated skin allergic response and chronic airway inflammation. The bone marrow-derived mast cells (BMMCs) of IL-27Rα-deficient mouse showed greater high-affinity receptor Fc epsilon RI (FcεRI)-mediated activation with significantly enhanced degranulation and cytokine production. Mechanistically, the dysregulated signaling in IL-27Rα–/– mast cells is associated with increased activation of Grb2-PLC-γ1-SLP-76, PI3K/Akt/IκBα signaling and decreased phosphorylation level of SH2 domain-containing protein phosphatase1 (SHP1). Furthermore, IL-27 treatment could inhibit mast cell activation directly, and retrovirus-based IL-27 expression in lung attenuated the airway inflammation in mice. Collectively, our findings reveal that IL-27 signaling negatively regulates mast cell activation and its mediated allergic response.
Concurrent stimulation of monocytes with CSF1 and polarizing cytokines reveals phenotypic and functional differences with classical polarized macrophages
Authors: An Liying , Michaeli Julia , Pallavi Prama , Breedijk Annette , Xu Xin , Dietrich Nadine , Sigl Martin , Keese Michael , Nitschke Katja , Jarczyk Jonas , Nuhn Philipp , Krämer Bernhard K , Yard Benito A , Leipe Jan 
Published: January 2022

AbstractIn atherosclerotic lesions, macrophages are exposed to CSFs and various microenvironmental cues, which ultimately drive their polarization state. We studied the expression of different CSFs in artery specimen and cultured vascular cells and assessed whether concurrent stimulation (CS) of monocytes with CSF1 and polarizing cytokines generated macrophages (CSM1 and CSM2) that were phenotypically and functionally different from classically polarized M1 and M2 macrophages. We also assessed the influence of acetylsalicylic acid (ASA) on the capacity of polarized macrophages to stimulate T-cell proliferation. CSF1 was the most prominent CSF expressed in arteries and cultured vascular cells. M1 and CSM1 macrophages differed in CD86 and CD14 expression, which was up-regulated respectively down-regulated by LPS. M2 and CSM2 macrophages were phenotypically similar. Cyclooxygenase expression was different in CSM1 (COX-1− and COX-2+ after LPS stimulation) and CSM2 (COX-1+ and COX-2−) macrophages. TNFα production was more pronounced in CSM1 macrophages, whereas IL-10 was produced at higher levels by CSM2 macrophages. Proliferation of allogeneic T cells was strongly supported by CSM2, but not by CSM1 polarized macrophages. Although ASA did not affect anti-CD3/CD28-mediated proliferation, it significantly reduced CSM2 and CSM1-mediated T-cell proliferation. Supernatants of LPS-stimulated CSM2 but not of CSM1 macrophages could overcome the inhibition by ASA. Hence, we demonstrate that CSM1 and CSM2 macrophages are phenotypically and to some extent functionally distinct from classically polarized M1 and M2 macrophages. CSM2 macrophages produce a COX-1-dependent soluble factor that supports T-cell proliferation, the identity hereof is still elusive and warrants further studies.
Comparative Analysis of Blood T2 Values Measured by T2‐TRIR and TRUST
Authors: Baas Koen P. A. , Coolen Bram F. , Petersen Esben T. , Biemond Bart J. , Strijkers Gustav J. , Nederveen Aart J. 
Published: January 2022

BackgroundVenous blood oxygenation (Yv), which can be derived from venous blood T2 (T2b), combined with oxygen‐extraction fraction (OEF) and cerebral metabolic rate of oxygen, is considered indicative for tissue viability and brain functioning and frequently assessed in patients with sickle cell disease. Recently, T2‐Prepared‐Blood‐Relaxation‐Imaging‐with‐Inversion‐Recovery (T2‐TRIR) was introduced allowing for simultaneous measurements of blood T2 and T1 (T1b), potentially improving Yv estimation by overcoming the need to estimate hematocrit.PurposeTo optimize and compare T2‐TRIR with T2‐relaxation‐under‐spin‐tagging (TRUST) sequence.Study TypeProspective.PopulationA total of 12 healthy volunteers (six female, 27 ± 3 years old) and 7 patients with sickle cell disease (five female, 32 ± 12 years old).Field Strength/Sequence3 T; turbo field echo planar imaging (TFEPI), echo planar imaging (EPI), and fast field echo (FFE).AssessmentT2b, Yv, and OEF from TRUST and T2‐TRIR were compared and T2‐TRIR‐derived T1b was assessed. Within‐ and between‐session repeatability was quantified in the controls, whereas sensitivity to hemodynamic changes after acetazolamide (ACZ) administration was assessed in the patients.Statistical TestsShapiro–Wilk, one‐sample and paired‐sample t‐test, repeated measures ANOVA, mixed linear model, Bland–Altman analysis and correlation analysis. Sidak multiple‐comparison correction was performed. Significance level was 0.05.ResultsIn controls, T2b from T2‐TRIR (70 ± 11 msec) was higher compared to TRUST (60 ± 8 msec). In patients, T2b values were lower pre‐ compared to post‐ACZ administration (TRUST: 80 ± 15 msec and 106 ± 23 msec and T2‐TRIR: 95 ± 21 msec and 125 ± 36 msec). Consequently, Yv and OEF were lower and higher pre‐ compared to post‐ACZ administration (TRUST Yv: 68% ± 7% and 77% ± 8%, T2‐TRIR Yv: 74% ± 8% and 80% ± 6%, TRUST OEF: 30% ± 7% and 21% ± 8%, and T2‐TRIR OEF: 25% ± 8% and 18% ± 6%).Data ConclusionTRUST and T2‐TRIR are reproducible, but T2‐TRIR‐derived T2b values are significantly higher compared to TRUST, resulting in higher Yv and lower OEF estimates. This bias might be considered when evaluating cerebral oxygen homeostasis.Evidence Level2Technical EfficacyStage 2
G‐quadruplex ligands inhibit chikungunya virus replication
Authors: Lv Lu , Cui Haoran , Chen Zhiyang , Zhou Yixuan , Zhang Leiliang 
Published: January 2022

AbstractChikungunya virus (CHIKV) is a mosquito‐transmitted alphavirus affecting human health globally. G‐quadruplex secondary structures attract great attention as potential targets for antiviral strategy. In this study, we show that the CHIKV genome possesses several conserved potential G‐quadruplex sequences. G‐quadruplex ligands BRACO‐19 and TMPyP4 could stabilize the CHIKV G‐quadruplex and inhibit the transcription of constructs containing CHIKV G‐quadruplex sequences. Importantly, BRACO‐19 and TMPyP4 suppress CHIKV replication. Our study not only reinforces the presence of viral G‐quadruplex sequences but also suggests that targeting G‐quadruplex structure could represent a novel strategy to inhibit CHIKV.
Redondoviridae infection regulates circRNAome in periodontitis
Authors: Zhang Yu , Wang Chunmei , Zhu Ce , Ye Wei , Gu Qin , Shu Chenbin , Feng Xiping , Chen Xi , Zhang Wen , Shan Tongling 
Published: January 2022

AbstractRedondoviridae is a recently identified family of DNA viruses associated with periodontitis. Circular RNAs (circRNAs) are novel endogenous, conserved noncoding RNAs contributing to the virus‐related immune‐inflammatory response. This study aimed to analyze the expression profiles of circRNAs in the gingival tissues of periodontitis patients with and without Redondoviridae‐infection and healthy controls using high‐throughput RNA sequencing combined with experimental validation. Out of 17 819 circRNAs, 175 were dysregulated. Functional annotation and enrichment analysis of the differential circRNA host genes demonstrated potential alterations in the molecular and cellular components and metabolism in individuals suffering from periodontitis with Redondoviridae infection. Moreover, "axon guidance," "lysine biosynthesis," and "vascular endothelial growth factor signaling pathways" were significantly enriched in Redondoviridae‐infected gingivitis tissues. Furthermore, the key circRNAs (circCOL1A1, circAASS, circPTK2, circATP2B4, circDOCK1, circTTBK2, and circMCTP2) associated with the pathobiology of Redondoviridae‐related periodontitis were identified by constructing circRNA–micro RNA (miRNA)–messenger RNA (mRNA) networks. Bioinformatics analyses demonstrated that abnormally expressed circRNAs might contribute to the etiopathogenesis and development of Redondoviridae‐related periodontitis. This study's findings have enhanced the current understanding of the Redondoviridae‐related periodontitis mechanism and provide insights into further applications for diagnostic markers and therapeutic uses.
Enhancement of toyocamycin production through increasing supply of precursor GTP in Streptomyces diastatochromogenes 1628
Authors: Zhang Jinyao , Xu Jie , Li Huijie , Zhang Yongyong , Ma Zheng , Bechthold Andreas , Yu Xiaoping 
Published: January 2022

AbstractThe nucleoside antibiotic toyocamycin (TM), which is produced by Streptomyces diastatochromogenes 1628, exhibits potent activity against a broad range of phytopathogenic fungi. TM was synthesized through a multi‐step reaction, using guanosine triphosphate (GTP) as precursor. Based on a comparison of proteomics data from S. diastatochromogenes 1628 and rifamycin‐resistant mutant 1628‐T15 with high yield of TM, we determined that the differentially expressed protein X0NBV6 called ribose‐phosphate pyrophosphokinase (RHP), which is a rate‐limiting enzyme involved in the de novo biosynthesis of GTP, exhibits a higher expression level in mutant 1628‐T15. In this study, to elucidate the relationships between RHP, GTP, and TM production, the gene rhp

sd
encoding RHP was cloned and overexpressed in S. diastatochromogenes strain 1628. The recombinant strain S. diastatochromogenes 1628‐RHP exhibited better performance at the transcriptional level of the rhp

sd
gene, as well as RHP enzymatic activity, intracellular GTP concentration, and TM production, compared to S. diastatochromogenes 1628. Finally, the yield of TM produced by S. diastatochromogenes 1628‐RHP (340.2 mg/L) was 133.3% higher than that produced by S. diastatochromogenes1628. Moreover, the transcriptional level of toy genes involved in TM biosynthesis was enhanced due to the overexpression of the rhp

sd
gene.
Foot radiographic angle variation as a function of weightbearing magnitude
Authors: Kimura Tadashi , Thorhauer Eric D. , Sangeorzan Bruce J. , Ledoux William R. 
Published: January 2022

AbstractWeightbearing radiographs are widely used to investigate foot disorders. However, it is unclear how imaging during partial weightbearing affects foot alignment measurements. This study aimed to determine a partial weightbearing threshold that yields consistent measurements of various radiographic angles. Eighteen normal fresh‐frozen cadaveric foot specimens were dissected and prepared for mechanical testing using a custom‐designed, computed tomography‐compatible loading frame. Specimens were placed in a neutral ankle position and scanned in five axial loading conditions (0%, 12.5%, 25%, 37.5%, and 50% bodyweight) using weightbearing computed tomography. (Note 50% bodyweight per foot represents full bodyweight in quiet stance.) The lateral first talometatarsal and calcaneal pitch angles were measured on lateral radiographic projections, and the hallux valgus angle and first−second, fourth−fifth, and first−fifth intermetatarsal angles were measured on axial projection images. The lateral first talometatarsal angle decreased significantly with increased bodyweight loading (p < 0.01). Mean significant decreases in the lateral first talometatarsal angle compared to 0% were 6.6° for 12.5%, 7.6° for 25%, 8.8° for 37.5%, and 10.0° for 50% bodyweight loading; 12.5% to 50% was also significant. There was no significant differences between other loading condition pairings or with increased axial load at other angles. The medial longitudinal arch flattened with increasing axial load, resulting in a decreased lateral first talometatarsal angle. However, this radiographic parameter did not change between the 25% and 50% bodyweight conditions, indicating that partial weightbearing imaging (between 12.5% and 25% bodyweight) might be enough to reproduce the sagittal foot alignments observed under full weightbearing conditions in normal feet.
Raman investigation of local photo‐bleaching in TDBC dye layer for photonics applications
Authors: Gassenq Alban , Pipon Yves , Montagnac Gilles , Boisron Olivier , Martinez Valérie , Bard Antoine , Benoit Jean‐Michel , Symonds Clémentine , Bellessa Joel 
Published: January 2022

AbstractLocal photo‐bleaching in dye layers is a promising method to pattern organic emitters for photonics applications like strong coupling researches or wavelength selective grating fabrication with tetrachloro diethyl benzimidazolo carbocyanine (TDBC) J‐aggregated layers. However, the understanding of the material change with bleaching in such layers is still ambiguous, which limits this method to fully exploit its potential. Raman spectroscopy is a fast, nondestructive, and readily technique to probe a material with micrometer spatial resolution, but the conditions to explore bleached TDBC layers are not well known. In this work, we have investigated active and bleached TDBC layers by Raman spectroscopy at different wavelengths in correlation with their optical properties. For active TDBC, Raman vibrations are well evidenced only in resonant configuration with suitable probe wavelengths (i.e., when layer absorption is high and emission is low). For bleached TDBC, because layer absorption is limited to the ultraviolet (UV) range, Raman peaks are observed only under UV illumination with similar transitions compared with active layer that indicates that TDBC molecules are barely affected by the bleaching mechanism, even if the optical properties are changed around the fundamental transition. Such assumption was confirmed by X‐ray photoelectron spectroscopy (XPS) measurements indicating close stoichiometry and limited changes in the N chemical bonds between active and bleached materials. This study allows a better understanding of the local photo‐bleaching in TDBC dye layer for photonics applications and highlights the deep UV Raman spectroscopy as relevant tool for studying bleached organic emitters.
Effects of wilting during a cloudy day and storage temperature on the fermentation quality and microbial community of Napier grass silage
Authors: Tian Jing , Yin Xiang , Zhang Jianguo 
Published: January 2022

AbstractBACKGROUNDWilting affects the chemical compositions and epiphytic microorganisms of grasses to some extent, thereby influencing the fermentation characteristics of silage. The present study investigated the effects of wilting during a cloudy day and ensiling temperature (20, 30 and 40 °C) on the fermentation of Napier grass (Pennisetum purpureum).RESULTSWilting during a cloudy day significantly decreased the water‐soluble carbohydrate content (P < 0.05) and significantly increased pH, counts of lactic acid bacteria and aerobic bacteria in grass (P < 0.05). Wilting increased the relative abundance of the Enterobacteriaceae family, as well as genera such as Acinetobacter, Pantoea and Lactococcus, and also decreased that of Sphingomonas and Methylobacterium, in fresh grass. Although ensiling increased the relative abundance of Lactobacillus, a Enterobacteriaceae genus was the dominant microorganism in Napier grass before ensiling and after ensiling. Wilting increased pH, acetic acid, butyric acid and NH3‐N contents, and also reduced the lactic acid content, of Napier grass silage. Additionally, the fermentation quality of silages at 30 °C was poorer, irrespective of wilting, as indicated by high pH, high butyric acid and NH3‐N contents, high relative abundance of Clostridium and Bifidobacterium, and a low lactic acid content. Ensiling at 40 °C enhanced lactic acid bacteria fermentation and also suppressed clostridial fermentation and the growth of microorganisms in silages.CONCLUSIONNapier grass should be ensiled immediately after being harvested during a cloudy day and ensiling at 30 °C should be avoided to prevent poor fermentation. © 2022 Society of Chemical Industry.