bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neighbors’ use of water and sanitation facilities can affect children’s health: a cohort study in Mozambique using a spatial approach
Authors: Grau-Pujol Berta , Cano Jorge , Marti-Soler Helena , Casellas Aina , Giorgi Emanuele , Nhacolo Ariel , Saute Francisco , Giné Ricard , Quintó Llorenç , Sacoor Charfudin , Muñoz Jose 
Published: May 2022

AbstractBackgroundImpact evaluation of most water, sanitation and hygiene (WASH) interventions in health are user-centered. However, recent research discussed WASH herd protection – community WASH coverage could protect neighboring households. We evaluated the effect of water and sanitation used in the household and by household neighbors in children’s morbidity and mortality using recorded health data.MethodsWe conducted a retrospective cohort including 61,333 children from a district in Mozambique during 2012–2015. We obtained water and sanitation household data and morbidity data from Manhiça Health Research Centre surveillance system. To evaluate herd protection, we estimated the density of household neighbors with improved facilities using a Kernel Density Estimator. We fitted negative binomial adjusted regression models to assess the minimum children-based incidence rates for every morbidity indicator, and Cox regression models for mortality.ResultsHousehold use of unimproved water and sanitation displayed a higher rate of outpatient visit, diarrhea, malaria, and anemia. Households with unimproved water and sanitation surrounded by neighbors with improved water and sanitation high coverage were associated with a lower rate of outpatient visit, malaria, anemia, and malnutrition.ConclusionHousehold and neighbors’ access to improve water and sanitation can affect children’s health. Accounting for household WASH and herd protection in interventions’ evaluation could foster stakeholders’ investment and improve WASH related diseases control.Graphical AbstractDistribution of main water and sanitation facilities used during study period.
A comparative content analysis of newspaper coverage about extreme risk protection order policies in passing and non-passing US states
Authors: Aubel Amanda J. , Pallin Rocco , Knoepke Christopher E. , Wintemute Garen J. , Kravitz-Wirtz Nicole 
Published: May 2022

AbstractBackgroundExtreme risk protection order (ERPO) laws are a tool for firearm violence prevention (in effect in 19 states), often enacted in the wake of a public mass shooting when media coverage of gun violence tends to spike. We compared news media framing of ERPOs in states that passed and those that considered but did not pass such laws after the 2018 mass shooting in Parkland, Florida.MethodsWe conducted a content analysis of 244 newspaper articles about ERPOs, published in 2018, in three passing (FL, VT, RI) and three non-passing states (PA, OH, CO). Measures included language used, stakeholders mentioned, and scientific evidence cited. We use chi-square tests to compare the proportion of articles with each measure of interest in passing versus non-passing states.ResultsCompared to newspaper coverage of non-passing states, news articles about ERPOs in passing states more often used only official policy names for ERPOs (38% vs. 23%,p = .03), used less restrictive language such as “prevent” to describe the process of suspending firearm access (15% vs. 3%,p < .01), mentioned gun violence prevention advocacy groups (41% vs. 28%,p = .08), and referenced research on ERPOs (17% vs. 7%,p = .03). Articles about passing states also more often explicitly stated that a violent event was or could have been prevented by an ERPO (20% vs. 6%,p < .01).ConclusionsMedia messaging that frames gun violence as preventable, emphasizes identifiable markers of risk, and draws on data in conjunction with community wisdom may support ERPO policy passage. As more states consider ERPO legislation, especially given endorsement by the Biden-Harris administration, deeper knowledge about successful media framing of these life-saving policies can help shape public understandings and support.
Evaluation of the short-term curative effect of closed reduction in the treatment of developmental dysplasia of the hip based on three-dimensional magnetic resonance imaging finite element analysis
Authors: Liu Jiani , Gao Tianyang , Li Jia , Shan Hui , Pan Shinong 
Published: May 2022

Abstract
Background
Based on the Digital Imaging and Communications in Medicine (DICOM) data of three-dimensional magnetic resonance imaging (3D-MRI), finite element models of the hip joints of children with developmental dysplasia of the hip were established. The primary objectives included simulation and analysis of the finite element model pre- and post-closed reduction under different stances and loads, and evaluation of the size and distribution of von Mises stress in the acetabulum and femoral head pre- and post-operation and the short-term effects.

Methods
Acetabular index measurements of both the unaffected and affected sides were conducted, alongside International Hip Dysplasia Institute (IHDI) classification of the affected hip. Establishing the finite element model of both the affected and unaffected hips was based on the 3D-T1WI sequence DICOM data, using Mimics, 3-matic, and Ansys software, before and after closed reduction surgery. The size and distribution data of von Mises stress on the affected side of the acetabulum and femoral head were collected pre- and post-operation.

Results
The study indicated that the increasing acetabular index of the affected hip was directly proportional to the increasing severity based on IHDI classification (P < 0.05). Preoperative IHDI classification significantly correlated with the von Mises stress (r = 0.560–0.569, 0.562–0.564, P < 0.05). Under different stances and load conditions, the von Mises stress on the affected side post-operation was lower than that noted pre-operation (P < 0.01), while that on the acetabulum increased proportionally to the load. Although the magnitude and distribution of von Mises stress on the affected side of the acetabulum were similar to those on the healthy side post-operation, there were statistical differences between the two (P < 0.01). The von Mises stress of the lateral column of the femoral head post-operation was significantly lower than that noted pre-operation (P < 0.01). While the high-stress points of the lateral column disappeared post-operation, the von Mises stress was evenly distributed in the femoral head.

Conclusions
The 3D-MRI finite element could provide the von Mises stress value and distribution characteristics of the acetabulum and femoral head pre- and post-operation. Closed reduction can, therefore, improve the size and distribution of von Mises stress on the affected acetabulum and femoral head.
Study on the relationship between hormone and Lp(a) in Chinese overweight/obese patients
Authors: Chang Xiaona , Bian Nannan , Ding Xiaoyu , Li Jinman , An Yu , Wang Jiaxuan , Liu Jia , Wang Guang 
Published: May 2022

Abstract
Background
Obesity is a risk factor for metabolic diseases and often influences hormone change. Lipoprotein (a) (Lp(a)) is associated with various metabolic diseases, but there are few studies on the relationship between Lp(a) and hormones in obese patients. This study investigated the the relationship between Lp(a) and hormones in Chinese overweight/obese people.

Methods
A total of 410 overweight/obese patients (Body mass index (BMI) ≥ 25 kg/m2) were included and underwent sociodemographic data investigations and relevant clinical examinations. Lp(a) was analyzed by colorimetric enzymatic assays and hormone was measured with chemiluminescence immunoassay method. According to Lp(a) levels, they were categorized into 3 groups: the lower Lp(a) group (Lp(a) levels < 30 mg/dl), the moderate Lp(a) group (Lp(a) levels between 30 mg/dl and 120 mg/dl) and the higher Lp(a) group (Lp(a) levels > 120 mg/dl). The differences of hormone levels among the three groups were compared and the relationship between Lp(a) and hormones was analyzed by Spearman’s rank correlation.

Results
The higher Lp(a) group had significantly lower testosterone (TES) levels compared with the lower and moderate Lp(a) groups in the case of gender, age and BMI matching. Lp(a) concentration was negatively correlated with TES levels in all participants and the negative association between Lp(a) and TES levels was also observed when the analysis was stratified by gender. Additionally, the TES was statistically related with Lp(a) levels in the multiple linear regression model (95% confidence interval: − 0.451 to − 0.079).

Conclusions
TES levels was negatively associated with Lp(a) levels in Chinese overweight/obese patients.
Influence of needle working length and root canal curvature on irrigation: a computational fluid dynamics analysis based on a real tooth
Authors: Zhou Na , Huang Zhengqiu , Yu Mingzhou , Deng Shuli , Fu Baiping , Jin Hanhui 
Published: May 2022

Abstract
Backgrounds
To compare the irrigation efficiency with different needle working length and different root canal curvature based on a real unshaped root canal using computational fluid dynamics (CFD) method.

Methods
Images of the root canal of the maxillary first molar after being prepared to .04/15 were scanned using micro-CT, and then imported into the software for three-dimensional reconstruction. A palatal root canal with a curvature of 23.4° was selected as the experiment canal. The needle working length of the 30-G flat needle was 4.75 mm, 5 mm, 5.25 mm and 5.5 mm short of apical foramen respectively, the flow pattern, irrigation velocity, shear stress were compared. The modified curved canals with a curvature of 0°, 5°, 10°, 20° and 30°were reconstructed via software. The flat needle was replaced at the optical inserted depth, and key parameters of irrigation efficiency were analyzed.

Results
Decreased needle working length had a positive impact on irrigation efficiency. With the optimal needle working length, the replacement of the apical irrigation fluid, the effective velocity, and wall shear stress were significantly improved in more severely curved root canals. With the same needle working depth and analogous canal curvature, irrigation efficiency is higher in real canal than that of modified canal.

Conclusions
Short needle working depth, large curvature and the anomalous inner wall of canals help to improve irrigation efficiency.
Meeting ethical challenges with authenticity when engaging patients and families in end-of-life and palliative care research: a qualitative study
Authors: DeCamp Matthew , Alasmar Ahmed , Fischer Stacy , Kutner Jean S. 
Published: May 2022

Abstract
Background
Delivering high quality, patient- and family-centered care depends upon high quality end-of-life and palliative care (EOLPC) research. Engaging patients and families as advisors, partners, or co-investigators throughout the research lifecycle is widely regarded as critical to ensuring high quality research. Engagement is not only an ethical obligation, it also raises ethical challenges of its own. We conducted a qualitative study to understand ethical challenges and potential solutions when engaging patients and families in EOLPC research.

Methods
We recruited and interviewed 20 clinical investigators and 22 patients or family caregivers through the Palliative Care Research Cooperative Group (PCRC). Interview transcripts were analyzed using constructivist grounded theory methodology. Analysis sought to identify ethical challenges and potential solutions, as well as to synthesize findings into practical recommendations tailored to engaging patients and families in EOLPC research.

Results
Our study identified 8 ethical challenges considered unique to the EOLPC research context and 11 potential solutions to these challenges. The most frequently described ethical challenges included the need to minimize burdens of engagement for patients and caregivers, challenges of dealing with death and illness, and paternalism or “gatekeeping” (i.e., withholding the opportunity to participate from patients or caregivers). Investigators and patients or family caregivers conceptualized ethics challenges differently; several issues appeared to fall outside a traditional research ethics paradigm and more into the ethics of relationships. We synthesized these findings into 4 practical recommendations hypothesized to support authentic engagement.

Conclusions
Engaging patients and families in EOLPC research can raise unique ethical challenges. These challenges can be overcome to empower participation, minimize the unique burdens of EOLPC, and promote diversity. Whereas traditional research ethics tend to emphasize protecting research participants who may be vulnerable, an ethics approach based on authentic engagement that explores what it means for investigators and patients or family caregivers to be in a relationship may be needed. Future research is needed to explore this approach and test these recommendations in practice.
Creating Wikipedia articles on health and technology topics can empower the writers and benefit the community
Authors: Moscovici Khen , Arie Gal Ben , Shelef Ilan , Kalet Adina 
Published: May 2022

Abstract
Background
Publicly accessible information regarding imaging procedures is lacking, especially in non-English languages. Biomedical engineering students do not generally have opportunities to practice conveying scientific knowledge to the public.

Methods
As part of a Techniques and Clinical Usage of Medical Imaging Devices course, for extra credit, several biomedical engineering students choose to create and edit Wikipedia articles in the local language (Hebrew). The goal of this activity was to serve the local community, while improving students’ abilities and self-perception in reading and reporting scientific knowledge. Following task completion, individual interviews were conducted with the students to assess the impact of the task on student personal development, sense of meaning and their view of their role in educating the public.

Results
Most students considered the task meaningful and impactful on society. Additional academic credit was not perceived as the most important incentive for participating.

Conclusions
Medical and other professional schools should seek to include tasks such as writing Wikipedia articles in their curricula. Educational assignments that integrate academic work, student identity development and direct community benefit can have a long-term beneficial impact on learners and society.
The acceptability of integrated healthcare services for HIV and non-communicable diseases: experiences from patients and healthcare workers in Tanzania
Authors: Shayo Elizabeth H. , Kivuyo Sokoine , Seeley Janet , Bukenya Dominic , Karoli Peter , Mfinanga Sayoki Godfrey , Jaffar Shabbar , Van Hout Marie-Claire 
Published: May 2022

Abstract
Background
In sub-Saharan Africa, the prevalence of non-communicable diseases (NCDs) has risen sharply amidst a high burden of communicable diseases. An integrated approach to HIV and NCD care offers the potential of strengthening disease control programmes. We used qualitative methods to explore patients’ and care-providers’ experiences and perspectives on the acceptability of integrated care for HIV-infection, diabetes mellitus (DM), and hypertension (HT) in Tanzania.

Methods
A qualitative study was conducted in selected health facilities in Dar es Salaam and Coastal regions, which had started to provide integrated care and management for HIV, DM, and HT using a single research clinic for patients with one or more of these conditions. In-depth interviews were held with patients and healthcare providers at three time points: At enrolment (prior to the patient receiving integrated care, at the mid-line and at the study end). A minimum of 16 patients and 12 healthcare providers were sampled for each time point. Observation was also carried out in the respective clinics during pre- and mid-line phases. The Theoretical Framework of Acceptability (TFA) underpinned the structure and interpretation of the combined qualitative and observational data sets.

Results
Patients and healthcare providers revealed a positive attitude towards the integrated care delivery model at the mid-line and at study end-time points. High acceptability was related to increased exposure to service integration in terms of satisfaction with the clinic setup, seating arrangements and the provision of medical care services. Satisfaction also centred on the patients’ freedom to move from one service point to another, and to discuss the services and their own health status amongst themselves. Adherence to medication and scheduling of clinic appointments appeared central to the patient-provider relationship as an aspect in the provision of quality services. Multi-condition health education, patient time and cost-saving, and detection of undiagnosed disease conditions emerged as benefits. On the other hand, a few challenges included long waiting times and limited privacy in lower and periphery health facilities due to infrastructural limitations.

Conclusion
The study reveals a continued high level of acceptability of the integrated care model among study participants in Tanzania. This calls for evaluation in a larger and a comparative study. Nevertheless, much more concerted efforts are necessary to address structural challenges and maximise privacy and confidentiality.
Silencing of histone deacetylase 3 suppresses the development of esophageal squamous cell carcinoma through regulation of miR-494-mediated TGIF1
Authors: Yang Yang , Zhang Yuan , Lin Zongxiang , Wu Kai , He Zhanfeng , Zhu Dengyan , Zhao Jia , Zhang Chunyang , Fan Yuxia 
Published: May 2022

AbstractBackgroundDeacetylation of histones by histone deacetylase 3 (HDAC3) acts importantly in modulating apoptosis, DNA damage and cellular progression. Herein, we aimed to unravel the functional role of HDAC3 in a lethal disease, esophageal squamous cell carcinoma (ESCC).MethodsThe expression of HDAC3 in clinically collected ESCC tissues was determined by RT-qPCR and immunohistochemistry. As revealed from bioinformatics analysis, the putative relations between HDAC3 and microRNA-494 (miR-494) and between miR-494 and transforming growth factor beta (TGFβ)-inducing factor 1 (TGIF1) were further verified by chromatin immunoprecipitation and dual-luciferase reporter gene assay. Functional roles of shRNA-mediated depletion of HDAC3, miR-494 mimic and overexpressed TGIF1 were explored by gain- and loss-of-function assays with regard to ESCC cell biological behaviors. A nude mouse model of ESCC was developed for in vivo validation.ResultsHDAC3 was highly expressed in ESCC tissues, suggestive of poor prognosis while TGIF1 was upregulated and miR-494 was downregulated. Mechanistic investigation revealed that HDAC3 inhibited miR-494 expression and TGIF1 was a direct target of miR-494. Furthermore, silencing HDAC3 or overexpressing miR-494 was demonstrated to suppress aggressive phenotypes of ESCC cells both in vitro through the activated TGFβ signaling pathway and in vivo, while TGIF1 overexpression induced opposite results.ConclusionCollectively, our findings provided demonstration regarding the oncogenic property of HDAC3 in ESCC via the miR-494/TGIF1/TGFβ axis.
Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells
Authors: Lee Ju-Hyun , Yoo Geon , Choi Juhyun , Park Si-Hyung , Shin Hyogeun , Prasad Renuka , Lee Yeunehee , Ahn Mee Ryung , Cho Il-Joo , Sun Woong 
Published: May 2022

AbstractThe cerebral organoid (CO) model has been used in the study of various neurodegenerative diseases owing to its physiological implications. However, the CO model may only be representative of certain clinical findings in affected patients, while some features are not recapitulated. In this study, we found that neurons in the CO model from patients with Alzheimer’s disease were less responsive to depolarization, in contrast to previous reports. This difference may be partly attributed to the variations in brain spatial identity depending on the genetic background of the induced pluripotent stem cells. Our current observation raises concerns that the phenotypes observed in the CO model need to be carefully evaluated for their clinical implications.
Influenza vaccination coverage in pediatric population in Italy: an analysis of recent trends
Authors: D’Ambrosio Floriana , Lanza Teresa Eleonora , Messina Rosaria , Villani Leonardo , Pezzullo Angelo Maria , Ricciardi Walter , Rosano Aldo , Cadeddu Chiara 
Published: May 2022

Abstract
Background
Influenza is a major cause of morbidity, mortality and exacerbation of extant chronic disease worldwide. Influenza vaccination is thus fundamental to reduce the burden of disease. In this study, we describe the trend of influenza vaccination coverage in the seasons 2010/11–2020/21 among children aged < 2, 2–4 and 5–8 in Italy.

Methods
We analyzed the trend of influenza vaccination coverage in the pediatric population in Italy from the 2010/11 to the 2020/21 season at national and regional level and observed the incidence of influenza-like illness (ILI) in the pediatric population between 2010/11 and 2020/21.

Results
In the period 2010/11–2019/20 the highest value of coverage (4.5%) was reached in the age group 2–4 and 5–8 (season 2010/11 and 2011/12, respectively), while the lowest belonged to the < 2 group (1.1% in the season 2015/16). In the season 2020/2021 all the age groups reported a substantial increase of coverage compared with the previous season. The highest value (19.0%) was reported in the age group 2–4, followed by the group 5–8 and <  2 (13.1 and 9.2%, respectively). Considering the rates of annual ILI cases, the highest value for the 0–4 age group was 18.5% in the 2011/12 season; for the 5–14 age group, the highest value was 27.7% in the 2010/11 season.

Conclusions
Over the past 11 years pediatric influenza vaccination coverage in Italy has been low, with relevant differences across regions and seasons, albeit a general increase in coverage has been observed in the 2020/21 season. Universal influenza vaccination for children should be considered as a priority for the high incidence in this age group. Further research is needed to improve knowledge and comparability of coverage rates, and to identify the best practices for organizational models of delivery which can support the improvement of trends, the acceptability and accessibility by parents and awareness in stakeholders and decision makers.