bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between Depressive Symptoms and Comorbidities Among Elderly Diabetic Individuals in China
Authors: Qiao Luyao , Pan Xin , Li Tianpei , Yi Shouqin , Tang Zhenyu 
Published: January 2025

ABSTRACTBackgroundDiabetic individuals are at an increased risk of mental illness and comorbidities. However, the precise association between depressive symptoms and comorbidity remains uncertain. Our study aimed to investigate this relationship among elderly Chinese diabetic patients.MethodsData from the China Health and Retirement Longitudinal Study (CHARLS) in 2020 were utilized for the cross‐sectional analysis. Depressive status was defined as the dependent variable, while the presence, number, and type of comorbidities served as independent variables. Logistic regression analyses were performed, adjusting for potential demographic factors, and health status and functioning factors.ResultsOur findings indicate that diabetic patients with complications are more likely to experience depression. With the exception for dyslipidemia (OR = 1.195, 95% CI: 0.969, 1.475), individuals with hypertension, heart disease, stroke, kidney disease, memory‐related disease, or arthritis/rheumatism were prone to develop depressive status in the fully adjusted model. After adjusting for covariates, diabetic patients with memory‐related diseases exhibited the most pronounced association with depressive symptoms (OR = 2.673, 95% CI: 1.882, 3.797). Furthermore, an increasing number of depression‐related comorbidities strengthened the association (p < 0.05). Sensitivity analysis revealed that there were no significant differences stratified by gender or marital status (p < 0.05).ConclusionsIn the elderly diabetic population in China, the presence, number, and type of comorbidities were independently associated with depressive symptoms. Diabetic patients with memory‐related diseases displayed the highest likelihood of experiencing depressive status. These findings underscore the importance of implementing effective strategies for multimorbidity management in diabetic patients.
Endovascular Treatment of Mirror Aneurysms in Subarachnoid Hemorrhage Patients: Single Stage or Multiple Stage?
Authors: Song Yunfei , Song Guoqiang , Liu Guijing , Mao Limei , An Xiuhu , Peng Chao , Li Jian , Chen Yan , Li Hongwen , Hou Changkai , Wang Bangyue , Zhao Yan , Wang Xiangdong , Yin Gangfeng , Yang Xinyu 
Published: January 2025

ABSTRACTObjectiveThe study evaluated the effectiveness and safety of single‐stage versus multistage endovascular treatment in subarachnoid hemorrhage patients with Mirror Aneurysms.Materials and MethodsOur research team performed a prospective study, focusing on the radiographic and clinical data of patients diagnosed with subarachnoid hemorrhage, specifically those who presented with Mirror Aneurysms upon admission to our institutions. According to the different endovascular treatment stages, these patients were grouped into the multistage cohort and the single‐stage cohort.ResultA total of 216 aneurysms were identified among the 108 patients, with every patient having one ruptured aneurysm. The duration of follow‐up was 2 years in both groups. In the single‐stage cohort, all 114 aneurysms in 57 patients were managed during a single session. During the 2‐year follow‐up, it was observed that 49 patients achieved a modified Rankin Scale score ≤2. Five complications were encountered, including cerebral vasospasm in three patients, cerebral hemorrhage in one patient, and thromboembolism in one patient. In the multistage cohort, only the ruptured aneurysm (amounting to 51 in total) received treatment at the initial occurrence, while the remaining 51 aneurysms were addressed subsequently. Throughout the 2‐year follow‐up period, 46 subjects exhibited a modified Rankin scale score ≤2. Overall, four complications were documented, including cerebral vasospasm in two patients, a subarachnoid hemorrhage in one patient, and thromboembolism in one patient.ConclusionThe safety and effectiveness of both endovascular treatment groups have been verified for patients with Mirror Aneurysms suffering from subarachnoid hemorrhage. If feasible, single‐stage embolization should be considered a viable treatment option for these patients.
Age‐Specific Effect and Pathways of Risk Factors on the Risk of Incident Type 2 Diabetes: A 13‐Year Prospective Cohort Study
Authors: Tian Xue , Chen Shuohua , Zhang Yijun , Xu Qin , Xia Xue , Wu Shouling , Wang Anxin 
Published: January 2025

ABSTRACTAimTo assess risk profiles and pathways for incident type 2 diabetes by age at onset.Materials and MethodsBased on the Kailaun study, 92,020 participants without type 2 diabetes were enrolled and classified into four age‐onset groups as < 55, 55 to < 65, 65 to < 75, and ≥ 75 years. Clinical risk factors and serum biomarkers were examined. Cox regression and Bayesian network analysis were performed.ResultsOf the clinical risk factors, metabolic syndrome had the highest hazard ratio [HR] for type 2 diabetes onset at any age, ranging from 4.17; 95% confidence interval [CI], 3.89–4.06) at onset in those < 55 years to 2.97 (95% CI, 2.57–3.44) at onset in those ≥ 75 years. Among biomarkers, insulin resistance evaluated by triglyceride‐glucose index had the highest HR (1.66; 95% CI, 1.61–1.70) for onset < 55 years. In comparison, weaker but significant associations with diabetes in onset < 55 years were noted for most lipids, metabolic, and inflammatory biomarkers. Most risk factors had attenuated relative rates at older ages. Bayesian network showed that the most important pathway to incident diabetes was through insulin resistance and metabolic syndrome, with conditional probabilities ranging from 62.1% in onset < 55 years, and attenuated to 16.3% for onset ≥ 75 years.ConclusionsMost risk factors for diabetes had attenuated relative rates at older ages. Metabolic syndrome and insulin resistance, in addition to prediabetes, obesity, hypertension, and dyslipidemia, tended to be stronger risk factors.
Investigating the Effect of Capric Acid on Antibiotic‐Induced Autism‐Like Behavior in Rodents
Authors: Shekhar Nikhila , Thakur Ajit Kumar 
Published: January 2025

ABSTRACTOwing to the high prevalence of gastrointestinal dysfunction in patients, the gut–brain axis is considered to play a vital role in neurodevelopment diseases. Recent pieces of evidence have pointed to the usage of antibiotics at an early developmental stage to be a causative factor in autism due to its ability to induce critical changes in the gut microbiota. The purpose of the study is to determine the neuroprotective effect of capric acid (CA) on autism in antibiotic‐induced gut dysbiosis in rodents. In this study, the effect of CA was observed in penicillin V (31 mg/kg, p.o.) exposed animals by evaluating their autism‐like behavioral and biochemical parameters. The establishment of gut dysbiosis was confirmed by 16 RNA sequencing, and behavioral tests were performed. Subsequently, oxidative stress, cytokine levels, and mitochondrial complex activities in the hippocampus and prefrontal cortex were analyzed. It was observed that the administration of penicillin V during the perinatal period produced gut dysbiosis and long‐lasting changes in social behavior with symptoms of anxiety and depression and impaired learning and memory. Treatment with penicillin V also produced oxidative stress, mitochondrial dysfunction, and inflammation in the hippocampus and prefrontal cortex. Treatment with CA produced a positive effect on the alterations with maximum effects evident at 400 mg/kg, p.o. through amelioration of behavioral as well as biochemical changes. The current study concluded that CA could act as a likely candidate for the treatment and management of autism via modulation of gut dysbiosis‐induced neurobehavioral parameters, oxidative stress, mitochondrial dysfunction, and inflammatory markers.
Chemical profiling of coriander, garlic and their combination to highlight the synergistic effect of the profiled compounds through in vitro and in vivo bioassays
Authors: Boujbiha Mohamed Ali , Chahdoura Hassiba , Ziani Borhane Eddine Cherif , Hsouna Anis Ben , Snoussi Mejdi , M'hadheb Manel Ben , Bachari Khaldoun , Selmi Boulbaba , Kačániová Miroslava , Mnif Wissem , Flamini Guido , Mosbah Habib 
Published: January 2025

AbstractHydroethanolic extracts of coriander seeds (CE), garlic bulbs (GE), and their combination (CGE), were chemically profiled using HS‐SPME/GC–MS and LC‐Q‐TOF/MS–MS to assess volatile composition and to study phenolic molecules, respectively. Biological assays were conducted through in vitro and in vivo experiments to measure the EC50 of the antioxidant assays and the MIC/MBC/MFC values of the antibacterial/antifungal activities. Antioxidant combination Index (CI) and fractional inhibitory concentration index (FICI) values were further recorded. The acute oral toxicity, analgesic, and gastroprotective activities were evaluated in vivo on Wistar rats and Swiss albino mice. Caffeoyl quinic acid, feruloyl‐quinic acid, and caffeic acid derivatives (quercetin, apigenin, and luteolin‐O‐glycoside) together with monoterpene linalool, α‐pinene, and γ‐terpinene were found to be highly present in CE. Organosulfur compounds (allicin, S‐allyl‐l‐cysteine, allin, γ‐Glutamyl‐S‐allylcysteine, and allyl sulfide) were predominant in GE. All the profiled compounds were co‐present in CGE. In vivo assays responded in a dose‐dependent showing better activity mainly at 200 mg kg−1. Chromatographic analysis profiled various phenolic acids, flavonols and derivatives, monoterpene, and organosulfur compounds in the assessed extracts and their combinations. Bioassays' responses were found to be dose‐dependent with better scores recorded with CGE. Thus, a synergetic effect was significantly highlighted.
Clostridium difficile and gut health: Bacteria, the gut microbiome, and diet
Authors: Yang Hao , Wang Lamei , Villafuerte Gálvez Javier A. , Ma Xinru , Xu Hua , Wu Shengru , Yao Junhu , Chen Xinhua , Cao Yangchun 
Published: January 2025

AbstractClostridium difficile infection (CDI) is a growing global health threat, presenting significant challenges to public health. Advances in metabolomics and proteomics have enhanced our understanding of human physiology, particularly the influence of gut microbiota and dietary components on diseases like CDI. This review explores recent insights into CDI pathogenesis, including the infection cycle, toxin mechanisms, spore structure, germination, and therapeutic strategies targeting spore formation and germination. We also examine the role of gut microbiota and metabolites in CDI progression, along with the impact of diet on disease pathology through microbiota modulation and immune regulation. We aim to guide the development of precise, individualized dietary strategies to regulate gut microbiota and immune responses, offering innovative therapeutic options for CDI prevention.
Prevalence of burnout and impact of workload on physician wellness: A cross‐sectional survey of hospitalists in British Columbia, Canada
Authors: Yousefi Vandad 
Published: January 2025

AbstractBackgroundHospitalists in British Columbia care for a large percentage of hospitalized patients across 21 acute care facilities.ObjectiveWe aimed to characterize the demographic and work attributes of the workforce and to understand levels of burnout and the relationship between workload and job satisfaction.MethodsWe conducted a cross‐sectional survey of individuals participating in hospitalist programs in BC.ResultsAlmost all individuals (96%) were involved in the care of patients with COVID‐19 in 2021, the height of the pandemic. High rates of burnout were demonstrated among hospitalists, with a large number of providers planning to significantly reduce or stop their involvement in acute care. Regression analysis identified workload as an important factor associated with burnout. Older physicians, those who had been practising for longer, and those with moderate to high number of shifts were more likely to consider reducing their involvement with their programs.ConclusionsHigh levels of burnout are associated with a desire to reduce work involvement among BC hospitalists. Health system leaders need to consider factors contributing to burnout as a key aspect of broader health human resource planning efforts.
脳梗塞と壊死性好酸球性心筋炎を合併した好酸球性多発血管炎性肉芽腫症の1救命例(Eosinophilic granulomatosis with polyangiitis complicated by cerebral infarction and necrotizing eosinophilic myocarditis: a case report)
Authors: 澄子 佐々木 , 一暢 宇根 , 一輝 武市 , 弘喜 木下 , 雄 中西 , 龍一 中布 
Published: January 2025

要旨症例は40歳台の女性，来院5年前に気管支喘息と診断され吸入ステロイドで加療されていた。1か月前から喘息症状が悪化し，1週間前から両下肢のしびれが出現した。近医の血液検査で末梢血の好酸球数の増加を認め，当院へ紹介受診された。来院時，好酸球3万/μLの著明な増加と心筋逸脱酵素上昇を認め，好酸球性心筋炎を，背景疾患として好酸球性多発血管炎性肉芽腫症の存在を疑った。循環動態は安定しており入院翌日に心筋生検を行い，検査後からプレドニゾロン1mg/kg/dayでの加療を開始した。入院してからも呼吸循環動態の悪化はみられなかったが，第3病日早朝に突然心停止した。速やかに一時的ペースメーカー挿入と体外循環式心肺蘇生法を実施し，自己心拍は再開した。劇症型心筋炎として同日よりステロイドパルス療法を開始，以後は経過良好で第10病日にICUを退室した。リハビリ開始後，左下肢のしびれが出現した。頭部MRI検査で右前頭葉に新鮮脳梗塞巣を認め，心臓超音波検査では新たに左室心尖部の無収縮と左室内血栓を認めたため，心原性脳塞栓症と診断した。本症例の心筋障害の原因は組織への好酸球浸潤が主体であったが，心筋シンチグラフィーから好酸球増多に伴う二次性凝固異常の関与も考えられた。予後良好といわれる好酸球性心筋炎においても，急変や合併症発症の可能性を念頭に背景疾患や重症度を評価し，迅速かつ適切に診療を行うことが重要であると思われる。
Partial REBOA併用下でdamage control IVRを行い救命した膵十二指腸動脈瘤破裂の1例(Partial resuscitative endovascular balloon occlusion of the aorta with damage control interventional radiology for ruptured visceral artery aneurysm)
Authors: 幸詩 馬庭 , 千佳 鷹尾 , 稔 甲村 , 健一 野中 , 光彦 日下部 
Published: January 2025

要旨Resuscitative endovascular balloon occlusion of the aorta（REBOA）は外傷や産科出血などでの使用経験が多いが，腹部内臓動脈瘤破裂に対して使用された症例報告，さらに大動脈遮断時間，遮断強度を工夫するpartial REBOA（P–REBOA）の報告例は未だ少ない。症例は65歳の男性。突然の腹痛，嘔吐を主訴に救急搬送され，来院時よりショック状態であり造影CTで正中弓状靭帯圧迫症候群に起因する膵十二指腸動脈瘤破裂と診断した。REBOAによるpartial occlusionで出血コントロールした状態でdamage control interventional radiology（DCIR）として前下膵十二指腸動脈を上腸間膜動脈から近位塞栓した。遮断時間は95分と長時間であったが，遮断による虚血再灌流などの合併症なく経過し自宅退院した。腹腔内出血に対して確実な止血術まで時間を要する場合，P–REBOAによる出血コントロールは有用であり，DCIRを併用することで侵襲や合併症を最小限にした治療戦略となる。
Lemierre症候群の呼吸不全増悪に寄与する因子の検討：症例報告(Factors contributing to exacerbation of respiratory failure in Lemierre’s syndrome: A case series)
Authors: 佐衣子 小原 , 和茂 井上 , 浩明 高田 , 聡志 関 , 栄寿 長谷川 
Published: January 2025

要旨　Lemierre症候群は，咽頭感染から内頸静脈の血栓症を引き起こし，他臓器に敗血症性塞栓を来す症候群である。遠隔臓器病変としては肺病変が最も多く90%以上の症例に合併し，呼吸不全に至る症例も認める。当院で経験した膿胸を合併したLemierre症候群の2例に関して，呼吸不全が増悪しうる要因について検討した。2例のうち，1例では急激な呼吸不全の増悪を認めた。症例は30代の男性であり，主訴は発熱，咽頭痛，胸痛，呼吸苦であった。来院時，低酸素血症を認め，high flow nasal cannulaを使用した。第3病日に急激な胸水の増加，呼吸状態の悪化を認め，CTガイド下に両側肺底部の胸腔ドレナージを施行した。胸水からも口腔内常在菌が検出された。第8病日に右肺尖部の増加した胸水に対し，再度CTガイド下胸腔ドレナージを追加した。徐々に呼吸状態は改善し，第36病日に退院となった。Lemierre症候群では，敗血症性肺塞栓症や肺膿瘍が胸腔内に穿破し膿胸を生じることがある。急速に胸水が増加するときは膿胸の合併を考慮し，より早期から積極的なドレナージが必要である。
Electrophoretic Deposition of Gentamicin Into Titania Nanotubes Prevents Evidence of Infection in a Mouse Model of Periprosthetic Joint Infection
Authors: Hamilton John L. , Gianotti Sofia , Fischer Julia , Della Fara Greta , Impergre Amandine , De Vecchi Francesca , AbuAlia Mohammed , Fischer Alfons , Markovics Adrienn , Wimmer Markus A. 
Published: January 2025

ABSTRACTPeriprosthetic joint infection (PJI) is a leading cause and major complication of joint replacement failure. As opposed to standard‐of‐care systemic antibiotic prophylaxis for PJI, we developed and tested titanium femoral intramedullary implants with titania nanotubes (TNTs) coated with the antibiotic gentamicin and slow‐release agent chitosan through electrophoretic deposition (EPD) in a mouse model of PJI. We hypothesized that these implants would enable local gentamicin delivery to the implant surface and surgical site, effectively preventing bacterial colonization. In the mouse PJI model, C57BL/6 mice received implants with TNTs coated with chitosan (chitosan group; control group) or with TNTs coated with chitosan and gentamicin (chitosan + gentamicin group; experimental group). Following implant placement, the surgical site was inoculated with 1 × 103 CFUs of Xen36 bioluminescent Staphylococcus aureus. All the mice in the chitosan group and none in the chitosan + gentamicin group had evidence of infection based on CFU analysis and bioluminescence imaging through the 14‐day assessment postsurgery. Correspondingly, scanning electron microscopy analysis at the implant surface demonstrated bacterial biofilm only in the chitosan group. Furthermore, periosteal reaction and peri‐implant bone loss at the femur were significantly reduced in the chitosan + gentamicin group. The chitosan + gentamicin group had reduced pain behavior, improved weight‐bearing, and increased weight compared to the chitosan‐control group. This study provides preclinical evidence supporting the efficacy of implants with TNTs coated with chitosan and gentamicin through EPD for preventing bacterial colonization and biofilm formation in a mouse model of PJI.
Common Variable Immunodeficiency Associated With Noninfectious Pulmonary Complications and Its Treatment: Beyond Immunoglobulin Therapy
Authors: Parsons Austin J. , Franco‐Palacios Domingo , Kelly Bryan , Grafton Gillian , McIntosh Javardo , Coleman David , Abdul Hameed Asif M. , Sayf Alaa Abu 
Published: January 2025

ABSTRACTCommon variable immunodeficiency (CVID) is a type of primary immunodeficiency that presents as a heterogenous disorder characterized by hypogammaglobinemia, poor response to vaccines, recurrent sinopulmonary infections, and can have noninfectious systemic manifestations. We performed a single‐center, retrospective, observational study of five patients with noninfectious complications of CVID. All patients had CVID as defined by the European Society of Immunodeficiencies criteria and had received intravenous immunoglobulin therapy. There were multiple pulmonary manifestations of CVID including frequent pneumonias, bronchiectasis, granulomatous lung disease, and pulmonary hypertension. All our patients were treated with pulmonary vasodilators for severe precapillary pulmonary hypertension along with individualized immunosuppression regimen for interstitial lung disease. Despite treatment for interstitial lung disease and PH, their conditions worsened over 2–3 years with all patients progressing toward organ transplant evaluation. Idiopathic thrombocytopenia and non‐cirrhotic portal hypertension were common, with three patients probably suffering from nodular regenerative hyperplasia. Noninfectious complications of CVID can affect different organs and progress despite advanced therapies. Single or multiorgan transplantation is a treatment option for patients with end‐stage organ involvement refractory to medical therapy.
Effects of Climate-Driven Salinity Regimes on Disease Dynamics of the Eastern Oyster, a Key Estuarine Resource and Bioindicator
Authors: Savage Kelley B. , Palmer Terence A. , Montagna Paul A. , Beseres Pollack Jennifer 
Published: January 2025

AbstractThis chapter addresses the connections between long-term trends in climate variability, freshwater inflow dynamics, and salinity patterns and their effects on Perkinsus marinus infection of eastern oysters (Crassostrea virginica) at local and regional scales in Texas estuaries. Salinities were highest during droughts compared to normal and wet climatic conditions. At the local, within-estuary scale, salinities increased longitudinally from reefs closest to the freshwater inflow source to reefs closest to the Gulf of Mexico inlet. At the regional scale, salinities increased latitudinally moving from estuaries in the northeast to the southwest. Relationships between salinity and P. marinus infection levels were strongest for market size (≥76 mm) oysters. At the local scale, mean salinity had positive relationships with infection prevalence and severity of infection (= weighted prevalence) for market-sized oysters; salinity explained 94% of the variance in infection prevalence and 82% of the variance in severity of infection. Relationships at the regional scale were less strong; salinity was positively correlated with infection severity but not infection prevalence in market-sized oysters; salinity explained 64% of the variation in infection prevalence and 71% of variation in infection severity. Results were used to develop salinity recommendations of between 21.0 and 24.9 for maintaining low (≤50%) prevalence of P. marinus-infected oysters and low severity of infection (≤1.0) in order to support development of water resource management plans that account for variability in climate patterns.
High-affinity ssDNA aptamer and chemiluminescent aptasensor for TIMP-1 detection in human serum
Authors: Wang Qin , Li Yanli , Yao Lige , Li Huiqin , Zhang Liuyan , Wang Yingjie , Li Jiayin , Chen Tian , Chai Kun , Gao Junli , Gao Junshun , Su Li , Li Xueming 
Published: January 2025

Abstract
TIMP-1 (Tissue Inhibitor of Metalloproteinases-1) is a protein involved in regulating extracellular matrix (ECM) degradation. It is recognized as a significant biomarker for cancer diagnosis. This study aimed to develop and characterize a single-stranded DNA (ssDNA) aptamer targeting human TIMP-1 protein with high affinity and specificity. A magnetic beads-based SELEX process combined with qPCR was used to select aptamers over seven rounds. The enriched ssDNA library was analyzed using high-throughput sequencing to identify candidate sequences, and these sequences were characterized using surface plasmon resonance (SPR) and binding assays to evaluate their affinity and specificity. The selected ssDNA aptamer demonstrated a dissociation equilibrium constant (K
D) of 0.41 nM and a very slow off-rate, enabling effective capture of TIMP-1 in serum samples. Furthermore, a chemiluminescent aptasensor was developed for TIMP-1 detection, which exhibited high specificity and a broad linear detection range from 1 to 500 ng/mL in human serum. The developed ssDNA aptamer targeting TIMP-1 shows high affinity and specificity, and the chemiluminescent aptasensor demonstrates promising potential for clinical diagnosis of TIMP-1 levels in human serum.

Graphical abstract
MT-100, a human Tie2-agonistic antibody, improves penile neurovasculature in diabetic mice via the novel target Srpx2
Authors: Liu Fang-Yuan , Cho Young-Lai , Fridayana Fitri Rahma , Niloofar Lashkari , Vo Minh Nhat , Huang Yan , Limanjaya Anita , Kwon Mi-Hye , Ock Jiyeon , Lee Seon-Jin , Yin Guo Nan , Lee Nam-Kyung , Ryu Ji-Kan 
Published: January 2025

Abstract
Diabetes is an incurable, chronic disease that can lead to many complications, including angiopathy, peripheral neuropathy, and erectile dysfunction (ED). The angiopoietin-Tie2 signaling pathway plays a critical role in blood vessel development, formation, remodeling, and peripheral nerve regeneration. Therefore, strategies for activating the Tie2 signaling pathway have been developed as potential therapies for neurovascular diseases. Here, we developed a human Tie2-agonistic antibody (MT-100) that not only resists Ang-2 antagonism and activates Tie2 signaling but also regulates a novel target, sushi repeat-containing protein X-linked 2 (Srpx2). This regulation led to the survival of vascular and neuronal cells, a reduction in the production of reactive oxygen species (ROS), activation of the PI3K/AKT/eNOS signaling pathway, increased expression of neurotrophic factors, and ultimately alleviation of ED in diabetic mice. Our findings not only provide conclusive evidence that MT-100 is a promising therapeutic strategy for the treatment of diabetic ED but also suggest it has substantial clinical applications for other complications associated with diabetes.