bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pinolenic acid Mediated Anti-inflammatory and Immunometabolic Effects in Peripheral blood-derived Monocytes from patients with Rheumatoid Arthritis is Associated with Modulation of miRNA expression
Authors: Takala Rabaa , Ramji Dipak , Andrews Robert , Zhou You , Farhat Mustafa , Elmajee Mohammed , Rundle Shelley , Choy Ernest 
Published: January 2022

Abstract
Objectives: Pinolenic acid (PNLA), an omega-6 polyunsaturated fatty acid from pine nuts, has anti-inflammatory and anti-atherogenic effects. We aimed to investigate the actions of PNLA on activated purified monocytes from peripheral blood of patients with rheumatoid arthritis (RA).Methods: Flow cytometry was used to assess the intracellular expression of TNF-α, IL-6, IL-1β, and IL-8 in purified monocytes from patients with RA after lipopolysaccharide (LPS) stimulation with/without PNLA pre-treatment. The whole genomic transcriptomic (WGT) profile of PNLA-treated, and LPS-activated monocytes from patients with active RA was investigated by RNA-sequencing.Results: PNLA reduced percentage of monocytes expressing the cytokines TNF-α by 23% (p=0.048), IL-6 by 25% (p=0.011), IL-1β by 23% (p=0.050) and IL-8 by 19% (p=0.066). Canonical pathway analysis showed that PNLA inhibited oxidative phosphorylation (p= 9.14E-09) and mitochondrial dysfunction (p=4.18E-08), while the sirtuin (SIRTs) signalling pathway was activated (p=8.89E-06). Pathway analysis predicted upstream activation of peroxisome proliferator-activated receptors (PPARs), sirtuin3, and let7miRNA, which are anti-inflammatory and antioxidative. In contrast, DAP3, LIF and STAT3, which are involved in TNF-α, and IL-6 signal transduction, were inhibited. Many miRNAs were modulated by PNLA suggesting potential post-transcriptional regulation of metabolic and immune response that has not been described previously. Multiple miRNAs target pyruvate dehydrogenase kinase-4 (PDK4), single-immunoglobulin interleukin-1 receptor-related molecule (SIGIRR), mitochondrially encoded ATP synthase membrane subunit 6 (MT-ATP6) and acetyl-CoA acyltransferase 2 (ACAA2); genes implicated in cell metabolism, inflammation, and mitochondrial dysfunction.Conclusion: PNLA has anti-inflammatory and immune-metabolic effects on monocytes that are pathogenic in RA and atherosclerosis. Dietary PNLA supplementation may regulate key miRNAs that are involved in mitochondrial, metabolic, and inflammatory pathways.
Cell-Free Fat Extract Attenuate Osteoarthritis via Chondrocytes Regeneration and Macrophages Immunomodulation
Authors: jia zhuoxuan , Kang Bijun , Cai Yizuo , Chen Chingyu , Yu Zheyuan , Li Wei , Zhang Wenjie 
Published: January 2022

Abstract
Background: The prevalence of osteoarthritis (OA) is increasing, yet clinically effective and economical treatments are unavailable. We have previously proposed a cell-free fat extract (CEFFE) containing multiple cytokines, which possessed anti-apoptotic, anti-oxidative, and proliferation promotion functions, as a “cell-free” strategy. In this study, we aimed to evaluate the therapeutic effect of CEFFE in vivo and in vitro . Methods: In vivo study, sodium iodoacetate-induced OA rats were treated with CEFFE by intra-articular injections for 8 weeks. Behavioral experiments were performed every two weeks. Histological analyses, anti-type II collagen, and toluidine staining provided structural evaluation. Macrophage infiltration was assessed by anti-CD68 and anti-CD206 staining. In vitro study, the effect of CEFFE on macrophage polarization and secretory factors was evaluated by flow cytometry, immunofluorescence, and quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The effect of CEFFE on cartilage regeneration was accessed by cell counting kit-8 assay and qRT-PCR. The generation of reactive oxygen species (ROS) and levels of ROS-related enzymes were investigated by qRT-PCR and western blotting. Results: In rat models with sodium iodoacetate (MIA)-induced OA, CEFFE increased claw retraction pressure while decreasing bipedal pressure in a dose-depend manner. Moreover, CEFFE promoted cartilage structure restoration and increased the proportion of CD206 + macrophages in the synovium. In vitro , CEFFE decreased the proportion of CD86 + cells and reduced the expression of pro-inflammatory factors in LPS + IFN-γ induced Raw 264.7. In addition, CEFFE decreased the expression of interleukin-6 and ADAMTs-5 and promoted the expression of SOX-9 in mouse primary chondrocytes. Besides, CEFFE reduced the intracellular levels of reactive oxygen species in both in vitro models through regulating ROS-related enzymes. Conclusions: CEFFE inhibits the progression of OA by promoting cartilage regeneration and limiting low-grade joint inflammation.
Three-Dimensional Speckle Tracking Echocardiography To Evaluate Left Ventricular Function In Patients With Acute ST-Segment Elevation Myocardial Infarction After Percutaneous Coronary Intervention Following Tongxinluo Treatment
Authors: Tian Ruimeng , Feng Jia , Qin Wenjuan , Wang Zhen , Zhai Zijing , Huang Lei , Lu Guilin , Dong Shanshan 
Published: January 2022

Abstract
Objective: Bying comparing the correlation between three-dimensional speckle tracking echocardiography (3D-STE) and three-dimensional left ventricular ejection fraction (LVEF), to explore the 3D-STE to evaluate the left ventricle of patients with acute ST-segment elevation myocardial infarction (acute STEMI) after percutaneous coronary intervention (PCI) following routine treatment with Tongxinluo drugs. Methods: Altogether, 60 patients with acute STEMI and 30 healthy adults were selected, and the patients were randomly divided into the routine group and the Tongxinluo group, with 30 people in each group. All patients underwent PCI, and routine echocardiography and 3D-STE assessments were performed for each group 72 h after PCI and 12 months after PCI to obtain the following left ventricular related functional parameters: left ventricular end-diastolic diameter (LVEDD), end-ventricular septal end-diastolic thickness (IVSD), left ventricular posterior wall end-diastolic thickness (LVPWD), left ventricular short axis shortening fraction (LVFS), Simpson’s left ventricular ejection fraction (Simpson’s LVEF), three-dimensional left ventricular ejection fraction (3D-LVEF), global longitudinal strain (GLS), global circumferential strain (GCS), left ventricular twist angle (LVtw), Torsion (Tor), peak strain dispersion (PSD), and myocardial comprehensive index (MCI). The same parameters were collected in the control group, the results were compared, and the correlation analysis between GCS, GLS, LVtw, Tor, and MCIF, and 3D-LVE was performed. Results: Compared with the control group, the LVFS, LVEF (Simpson), 3D-LVEF, GLS, GCS, LVtw, Tor, and MCI significantly decreased in patients with STEMI after PCI, while the PSD significantly increased ( P <0.05). Compared with the values 72 h after PCI, the LVEDD, LVFS, LVEF (Simpson), 3D-LVEF, GLS, GCS, LVtw, Tor, and MCI significantly increased at 12 m after PCI, while PSD significantly decreased ( P <0.05). No significant difference was observed between the two groups at 72 h after PCI ( P >0.05). At 12 months after PCI, the LVEF, GLS, GCS, LVtw, Tor, and MCI of the Tongxinluo group were higher than those of the routine group. The PSD was significantly lower in the Tongxinluo group ( P <0.05). MCI and 3D-LVEF have the strongest correlation and highest consistency, which can best reflect the changes in the left ventricular function in patients with acute STEMI after PCI. Conclusion: 3D-STE can be used to evaluate the protective effect of Tongxinluo on the left ventricular function in patients with acute STEMI after PCI.
COVID-19: Exercise Behavior of College Students Communicating at Home
Authors: Li Rui , Pan Daoyong , Lu Songtao 
Published: January 2022

Abstract
Background: During the outbreak and spread of COVID-19, the extension of college students' time spent studying at home changed their physical exercise behavior and affected the physical activity behavior of the whole family.Methods: A questionnaire survey was conducted among 1,582 college students using a specific measurement scale. A total of 305 urban college students were selected as research subjects. SPSS24.0 and AMOS24.0 were used for statistical analysis.Results: During the COVID-19 transmission period, the pair correlation coefficients of exercise behavior, exercise attitude, and family exercise conditions were 0.63, 0.36, and 0.25, respectively. The influence on family exercise behavior is as follows: college students' exercise behavior (0.403), family exercise support (0.329), and college students' exercise attitude (0.257). The most significant influence on family exercise support is college students' exercise attitude (0.509). The regression model of family exercise behavior standardization had 0.74 and 0.44 explanatory power to family exercise behavior and family exercise support, respectively.Conclusions: The individual-level interventions were assessed by considering the interaction between individual exercise behavior and individual factors. In addition, the exercise environment exhibited a regulatory role and should be controlled. At the interpersonal level, the communication of the college students regarding exercise behavior was bidirectional. Exercise support for family members is an important factor affecting two-way communication and has a significant effect. With the development of the exercise behavior theory, the interaction between individuals is the origin of the spread of group behavior. The data suggest that instead of one-way influence two-way influence mechanisms should be proposed to assess the transformation from the individual to group exercise behavior.
Temperature Sensitivity Analysis of Dual Material Stack Gate Oxide Source Dielectric Pocket TFET
Authors: Nishad Dharmender , Nigam Kaushal , Kumar Satyendra 
Published: January 2022

Abstract
Temperature dependence performance variation is one of the major concerns in predicting the actual electrical characteristics of the device as the bandgap of semiconducting material varies with temperature. Therefore, in this article, for the first time, the impact of temperature variations ranging from 300K to 450K on the DC, analog/ radio frequency, and linearity performance of dual material stack gate oxide-source dielectric pocket-tunnel- field-effect transistor (DMSGO-SDP-TFET) is investigated. In this regard, technology computer-aided design (TCAD) simulator is used to analyze DC, and analog/radio frequency performance parameters such as carrier concentration, energy band variation, band to band tunneling rate, IDS - VGS characteristics, transconductance (gm), cut o frequency (f T ),gain-bandwidth product (GBP), maximum oscillating frequency (fmax), transconductance frequency product (TFP), and transit time considering the impact of temperature variations. Furthermore, linearity parameters such as third-order transconductance (gm3), third-order voltage intercept point (VIP3), third-order input-interception point (IIP3), and intermodulation distortion (IMD3) are also analyzed with temperature variations as these performance parameters are significant for linear and analog/radio frequency applications. Moreover, the performance of the proposed DMSGO- SDP-TFET is compared with the conventional dual-material stack gate oxide-tunnel- field-effect transistor (DMSGO-TFET). From the comparative analysis, in terms of % per kelvin, DMSGO-SDP-TFET demonstrates lesser sensitivity towards temperature variation. Hence, the proposed DMSGO-SDP-TFET can be a suitable candidate for low power switching and analog/radio frequency applications at elevated temperatures as compared to conventional DMSGO-TFET.
SARS-CoV-2 Vaccinated Breakthrough Infections With Fatal and Critical Outcomes in the Department of Antioquia, Colombia
Authors: Cardona Andres , Villegas Simon , Lopez Maria Stella , Maya Maria Angelica , Ortiz-Restrepo Celeny , Hernandez-Ortiz Olga , Ciuoderis Karl , Perez Laura , Betancour Idabely , Muñoz Carolina , Bustamante Lina , Rangel Andres Mauricio , Osorio Jorge , Hernandez-Ortiz Juan Pablo , Anderson Mark , Averhoff Francisco , Cloherty Gavin 
Published: January 2022

Abstract
The introduction of variants of concern and interest in the Departamento of Antioquia, Colombia, was concomitant with the beginning of the COVID-19 immunization program. Genomic surveillance indicates that none of the emerging variants –alpha, gamma, lambda, mu or delta– were dominant between January and August 2021. The immunization includes CoronaVac, BNT162b2, Ad26.COV2.S and ChAdOx1-S vaccines. By September 10th, 34.43% inhabitants were fully vaccinated. We characterized, SARS-CoV-2 breakthrough infections in 96 patients, 30 with fatal outcomes, 13 with ICU hospitalization and 53 with mild or asymptomatic disease. Even though gamma and mu variants co-circulated at similar levels, the latter was found to be predominant in patients with fatal outcomes and in those with ICU hospitalizations. We found a significant occurrence of the B.1.625 variant in these patients. Genetic substitutions of therapeutic and immunological concern, E484K and N501Y, are consistently observed in 90.1% and 79.5% of these variants, respectively. Evidence suggests that it is less probable to become infected after 60 days post-treatment with BNT162b2 than with CoronaVac. Importantly, we found that advanced age and comorbidities foster conditions for fatal and ICU outcomes in vaccinated patients. Our observations demonstrate the effectiveness of vaccination and identify patients with higher risks of subsequent breakthrough infections.
Comparison of Intestinal Stent Placement Followed By Laparoscopic Surgery and Emergency Surgery in Obstructive Colorectal Cancer: A Retrospective Analysis and Meta-analysis
Authors: Li Zhengwei , Lu Yan , Wang Kang , Liao Tianyou , Ju Yongle , Peng Yuanhong , Ouyang Manzhao 
Published: January 2022

Abstract
Background: For patients with colorectal cancer and malignant intestinal obstruction, it is still controversial to perform endoscopic intestinal stent placement followed by laparoscopic surgery. This study compares the endoscopic intestinal stent placement followed by laparoscopic surgery and emergency surgery in patients with colorectal cancer and malignant intestinal obstruction.Method: 11 compliant publications from Pubmed, Cochrane and Embase databases were analyzed using Revies Manager 5.2 software. SPSS 21 was used to retrospectively analyze 99 patients admitted to our center from 2014 to 2019.Results: There were significant differences between the two groups in three of the five criteria. In the SBTS group, the perioperative mortality rate was lower, with an OR of 0.46 (95% CI: 0.22-0.95, P=0.04), the incidence of postoperative wound infection was lower; OR was 0.44 (95% CI: 0.24-0.82, P=0.009); Postoperative hospital stay was shorter, MD was -2.07 (95% CI: -2.55--1.59, P<0.00001).Retrospective analysis of the clinical outcome differences between the SBTS group and ES group in our center: Compared to the ES group, the SBTS group displayed lower infection rate of surgical incision (χ2=3.94,P =0.04) ); no difference in the frequency of occurrence of anastomotic leakage (χ2=0.18,P=0.67), did not reduce perioperative mortality (χ2=0.94,P=0.33);shorter operating time (204.13±37.35 min) (t=5.08,P=0.000), lower intraoperative blood loss (155.65±94.90 ml) (t=3.90,P=0.001); and shorter postoperative hospital stay (12.91±5.47 d) (t=2.64, P=0.01).Conclusion: Compared the emergency surgery group, endoscopic intestinal stent placement followed by the laparoscopic surgery can reduce perioperative mortality, postoperative wound infection, intraoperative blood loss, and the length of postoperative hospital stay. There was no difference between the two methods as far as the incidence of posterior anastomotic leakage and operating time were concerned.
Effects of the Combination of High-intensity Interval Training and Ecdysterone on Learning and Memory Abilities, Antioxidant Enzymes Activities, and Neuronal Population in an Amyloid-beta-induced Rat Model of Alzheimer’s Disease
Authors: Gholipour Parsa , komaki alireza , Ramezani Mahdi 
Published: January 2022

Abstract
Aims: Oxidative stress and neuronal death are the primary reasons for the progression of amyloid-beta (Aβ) deposition and cognitive deficits in Alzheimer’s disease (AD). Ecdysterone (Ecdy), a common derivative of ecdysteroids, possesses free radical scavenging and cognitive-improving effects. High-intensity interval training (HIIT) may be a therapeutic strategy for improving cognitive decline and oxidative stress. The present study was aimed to evaluate the effect of HIIT alone and its combination with Ecdysterone on the changes in learning and memory functions, hippocampal antioxidant enzymes activities, and neuronal population after AD induced by Aβ in male rats.Materials and methods: Following ten days of Aβ-injection, HIIT exercise and Ecdysterone treatment (10 mg/kg/day; P.O.) were initiated and continued for eight consecutive weeks in rats. At the end of the treatment period, rat’s learning and memory functions were assessed using water-maze and passive-avoidance tests. Moreover, the activity of superoxide dismutase (SOD), catalase (CAT), Glutathione Peroxidase (GPx), Glutathione Reductase (GRx) and neuronal population were evaluated in rat’s brains.Results: The results indicated that Aβ injection disrupted spatial/passive avoidance learning and memory in both water-maze and passive-avoidance paradigms, accompanied by a decrease in the superoxide dismutase and catalase (as endogenous antioxidants) in rat hippocampus. Additionally, Aβ injection resulted in neuronal loss in the cerebral cortex and hippocampus. Although consumption of Ecdysterone separately improved spatial/passive avoidance learning and memory impairments, recovered hippocampal activity of SOD, CAT, GRx, GRx and prevented the hippocampal neuronal loss, its combination with HIIT resulted in a more powerful and effective amelioration in all the above-mentioned Aβ-neuropathological changes.Conclusion: The current work's data confirms that a combination of HIIT exercise and Ecdysterone treatment could be a promising potential therapeutic agent against AD-associated cognitive decline, owing to their free radical scavenging and neuroprotective properties.
Comparação do potencial erosivo de balas originais e azedas (“sour candies”)
Authors: Pegoretti Maria Luísa Raizer , Batista Nádia , Araújo Silvana Marchiori , Schmitt Beatriz Helena Eger , Silveira Eliane Garcia da , Farias Maria Mercês Aquino Gouveia 
Published: January 2022

Introdução: O consumo frequente de balas ácidas está associado à etiologia da erosão dental. Objetivo: Comparar o potencial erosivo de balas originais e azedas. Material e método: Analisaram-se balas originais e azedas nos sabores: morango, uva, frutas silvestres e dentadura, constituindo-se 4 grupos: G-1- balas originais dissolvidas em água duplamente deionizada; G-2- balas originais dissolvidas em saliva artificial; G-3- balas azedas dissolvidas em água duplamente deionizada; G-4- balas azedas dissolvidas em saliva artificial. O pH das soluções foi mensurado utilizando-se um potenciômetro e eletrodo combinado de vidro e a acidez titulável (AT) adicionando-se alíquotas de 100 μL NaOH 1M as soluções até alcançarem pH 5,5. A concentração de cálcio foi determinada utilizando um espectrofotômetro de absorção atômica. Os resultados foram submetidos à Análise de Variância (ANOVA). As médias de pH, AT foram comparadas pelo teste Tukey em um nível de 5% de significância. Resultados: Observou-se que as balas dissolvidas em água apresentaram valores de pH inferiores a 5,5, não havendo diferença significante entre os grupos (G-1) e (G-3). Na diluição em saliva artificial os sabores do grupo G-2 apresentaram valores de pH significantemente mais elevados que os sabores do grupo G-4, que mantiveram valores de pH inferiores a 5,5. Comparando-se a AT entre os grupos G-1 e G-3 observou-se que os sabores do grupo G-3 apresentaram uma AT significantemente mais alta. Conclusão: Todas as balas analisadas apresentam potencial erosivo, porém as balas “sour” ou azedas apresentaram baixo pH, maior AT e baixo teor de cálcio, apresentando maior potencial erosivo.
ANALISIS KOMUNIKASI CSR PERTAMINA EP CEPU PADA PROGRAM PENGEMBANGAN BUMDESA DI KABUPATEN BOJONEGORO
Authors: Taufiq Ahmad 
Published: January 2022

Abstrak
Penelitian komunikasi CSR Pertamina EP Cepu pada program Pengembangan BUMDes di Kabupaten Bojonegoro, Jawa Timur, mengacu Teori Komunikasi Lasswell yang mencakup lima indikator. Yakni, mengenal khalayak, penyusunan pesan, penetapan metode, seleksi dan penggunaan media, serta efek yang diharapkan. Penelitian menggunakan pendekatan kualitatif-deskriptif, dengan teknik penentuan informan melalui purposive samping. Analisis data mengacu model Miles dan Huberman, yang terdiri dari pengumpulan informasi, reduksi data, penyajian data, dan penarikan kesimpulan/verifikasi.
Hasil penelitian menunjukkan komunikasi program ke khalayak dijalankan dengan pola
need assesment dan koordinasi. Dalam dimensi komunikator, cara komunikasi program CSR melalui komunikasi kelompok dan komunikasi personal (informal) dengan karakter (kearifan) lokal dengan menggunakan media sinau bareng, ngopi bareng, jagong klobot, dan forum usai Shalat Jumat. Untuk dimensi efek, efektivitas komunikasi melahirkan dukungan dari pemdes dengan menghibahkan TKD dan penyertaan modal.
Kata Kunci: BUMDes, CSR, Komunikasi, Pengembangan, Program
The relationship between behavioral problems and screen time in children during COVID-19 school closures in Japan
Authors: Ueno Chika , Yamamoto Shuichi 
Published: January 2022

Abstract

Background and objectives
Studies have shown that COVID-19 school closures negatively impacted children’s well-being. We assessed the impact of school closures on children in Japan. We postulated that screen time and sleep habits during school closure would be associated with children’s mental health status.

Methods
A cross-sectional study was conducted with 959 children in four public elementary schools. Parents completed an original questionnaire in which we enquired about children’s behavioral problems, screen time (duration of watching TV/videos and duration of playing video games), sleep habits, and bodyweight change during school closure. Logistic regression analyses were performed to investigate the relationships between behavioral problems, bodyweight gain, and screen time.

Results
It was found that 50.3% of children exhibited some behavioral problems during school closure; fewer children exhibited such problems after schools reopened. Moreover, children’s behavioral problems during school closure were associated with longer total screen time (odds ratio [OR]: 1.2, 95% confidence interval [CI]: 1.14–1.28, p < .001). Sleep habits were not different between children with behavioral problems and those without. Of the children, 16.7% experienced bodyweight gain during school closure, which was associated with behavioral problems (OR: 2.0, 95% CI: 1.43–2.92, p < .001) and time spent watching TV/videos (OR: 1.2, 95% CI: 1.08–1.28, p < .001) during closure.

Conclusions
COVID-19 school closure negatively impacted school children physically and psychologically. Screen time was associated with both physical and mental health status. Therefore, children should not engage in arbitrary screen time during school closures and should be supported in their daily routines.