bluuebunny/crossref_metadata_2025_split huggingface dataset:
The SlTPL3–SlWUS module regulates multi‐locule formation in tomato by modulating auxin and gibberellin levels in the shoot apical meristem
Authors: Song Shiwei , Huang Binbin , Pan Zanlin , Zhong Qiuxiang , Yang Yinghua , Chen Da , Zhu Lisha , Hu Guojian , He Mi , Wu Caiyu , Zouine Mohammed , Chen Riyuan , Bouzayen Mondher , Hao Yanwei 
Published: August 2022

ABSTRACTMalformed fruits depreciate a plant's market value. In tomato (Solanum lycopersicum), fruit malformation is associated with the multi‐locule trait, which involves genes regulating shoot apical meristem (SAM) development. The expression pattern of TOPLESS3 (SlTPL3) throughout SAM development prompted us to investigate its functional significance via RNA interference (RNAi) and clustered regularly interspaced short palindromic repeats/CRISPR‐associated nuclease 9 (Cas9)‐mediated gene editing. Lower SlTPL3 transcript levels resulted in larger fruits with more locules and larger SAMs at the 5 d after germination (DAG5) stage. Differentially expressed genes in the SAM of wild‐type (WT) and SlTPL3‐RNAi plants, identified by transcriptome deep sequencing (RNA‐seq), were enriched in the gibberellin (GA) biosynthesis and plant hormone signaling pathways. Moreover, exogenous auxin and paclobutrazol treatments rescued the multi‐locule phenotype, indicating that SlTPL3 affects SAM size by mediating auxin and GA levels in the SAM. Furthermore, SlTPL3 interacted with WUSCHEL (SlWUS), which plays an important role in SAM size maintenance. We conducted RNA‐seq and DNA affinity purification followed by sequencing (DAP‐seq) analyses to identify the genes regulated by SlTPL3 and SlWUS in the SAM and to determine how they regulate SAM size. We detected 24 overlapping genes regulated by SlTPL3 and SlWUS and harboring an SlWUS‐binding motif in their promoters. Furthermore, functional annotation revealed a notable enrichment for functions in auxin transport, auxin signal transduction, and GA biosynthesis. Dual‐luciferase assays also revealed that SlTPL3 enhances SlWUS‐mediated regulation (repression and activation) of SlPIN3 and SlGA2ox4 transcription, indicating that the SlTPL3–SlWUS module regulates SAM size by mediating auxin distribution and GA levels, and perturbations of this module result in enlarged SAM. These results provide novel insights into the molecular mechanism of SAM maintenance and locule formation in tomato and highlight the SlTPL3–SlWUS module as a key regulator.
Quantitative proteomics reveals redox‐based functional regulation of photosynthesis under fluctuating light in plants
Authors: Chen Qi , Xiao Yixian , Ming Yu , Peng Rong , Hu Jiliang , Wang Hong‐Bin , Jin Hong‐Lei 
Published: August 2022

ABSTRACTPhotosynthesis involves a series of redox reactions and is the major source of reactive oxygen species in plant cells. Fluctuating light (FL) levels, which occur commonly in natural environments, affect photosynthesis; however, little is known about the specific effects of FL on the redox regulation of photosynthesis. Here, we performed global quantitative mapping of the Arabidopsis thaliana cysteine thiol redox proteome under constant light and FL conditions. We identified 8857 redox‐switched thiols in 4350 proteins, and 1501 proteins that are differentially modified depending on light conditions. Notably, proteins related to photosynthesis, especially photosystem I (PSI), are operational thiol‐switching hotspots. Exposure of wild‐type A. thaliana to FL resulted in decreased PSI abundance, stability, and activity. Interestingly, in response to PSI photodamage, more of the PSI assembly factor PSA3 dynamically switches to the reduced state. Furthermore, the Cys199 and Cys200 sites in PSA3 are necessary for its full function. Moreover, thioredoxin m (Trx m) proteins play roles in redox switching of PSA3, and are required for PSI activity and photosynthesis. This study thus reveals a mechanism for redox‐based regulation of PSI under FL, and provides insight into the dynamic acclimation of photosynthesis in a changing environment.
Effects of dietary supplementation with microbial phytase on the growth, bone minerals, antioxidant status, innate immunity and disease resistance of African catfish fed on high soybean meal‐based diets
Authors: Adeshina Ibrahim , Akpoilih Benjamin U. , Tiamiyu Lateef O. , Badmos Abdulhameed A. , Emikpe Benjamin O. , Abdel‐Tawwab Mohsen 
Published: August 2022

AbstractThe microbial phytase, derived from Buttiauxella gaviniae, Yersinia mollarettiv and Hafnia spp., is proven to be safe for avian and porcine feeding and promotes their overall growth performance. Here, we have evaluated microbial phytase's effects on the growth, bone mineral content, antioxidant status, immune responses and the resistance of African catfish (Clarias gariepinus) fed with high soybean meal‐based diets against Aeromonas hydrophila infection. Five isonitrogenous diets (40% protein) were supplemented with different levels of microbial phytase ranging from 0 as a control to 250, 500, 750 and 1000 FTU/kg diet. African catfish (n = 300; 8.5 ± 0.3 g) were allocated in 15 50‐L tanks (in triplicates) and were fed on the prepared tested diets for 12 weeks. After the end of the feeding period of 12 weeks, 10 fish from each replicate was intraperitoneally infected with A. hydrophila (0.5 × 105 CFU/ml) and monitored for 14 days. Dietary phytase levels linearly and quadratically improved the growth performance of African catfish and stimulated feed intake. Bone levels of calcium, phosphorus, magnesium and zinc were also positively modulated in phytase‐fed fish, especially at 750–1000 FTU/kg diet. Similarly, counts of red and white blood cells as well as haemoglobin, packed cells volume, platelets, lymphocytes and heterocytes were significantly modulated in all fish fed with phytase‐supplemented diets. Higher levels of serum total protein, albumin and globulin were also observed in fish fed with a 750–1000 FTU/kg diet of phytase. Conversely, aspartate and alanine aminotransferase activities were lower in fish fed with a 750–1000 FTU/kg diet of phytase, compared to those fed the control diet. Moreover, antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), reduced glutathione levels and immune responses (lysozyme, respiratory burst, protease and alkaline phosphatase activities) biomarkers were linearly and quadratically elevated, while malondialdehyde values were linearly and quadratically decreased in fish groups fed with phytase‐based diets. After administering A. hydrophila, 60.0% of the fish fed the control diet perished, while no mortalities were observed in fish fed with 750–1000 FTU/kg diets. Taken together, the current study reveals that dietary phytase could improve the growth performance, blood profile, bone mineralization, antioxidant activities, immunity and overall protection of African catfish against A. hydrophila infection. Dietary phytase may be efficiently used in the feeding of African catfish to enhance their overall performance and mitigate health conditions with optimum level of 900 FTU/kg diet.
Explaining behavioral health differences in urban and rural sexual minority adolescents
Authors: Goldbach Jeremy T. , Parra Luis A. , O'Brien Rory P. , Rhoades Harmony , Schrager Sheree M. 
Published: August 2022

AbstractPurposeExperiences of sexuality‐based discrimination (ie, minority stressors) against youth who identify as nonheterosexual (ie, sexual minority) have been associated with increased symptoms of anxiety, depression, and post‐traumatic stress disorder (PTSD) for sexual minority adolescents (SMA; ages 14‐17). However, little is known about the experiences of SMA living in rural communities across the United States. Thus, the present study sought to examine differences in mental health patterns between urban and rural dwelling SMA, and to see whether these differences are, at least in part, explained by experiences of lifetime minority stress.MethodsA nationwide sample of SMA residing in the United States (N = 2,558; aged 14‐17, M = 15.90 years, SD = 0.98) was recruited through purposive social media and respondent‐driven sampling methods to complete a cross‐sectional survey online. Measures included those of minority stress, urbanicity, and symptoms of anxiety, depression, and PTSD. Parallel multiple mediation (PMM) analysis was employed to test whether urbanicity was associated with anxiety, depressive, and PTSD symptoms through reported lifetime minority stress.FindingsOn average, SMA living in rural areas significantly reported more lifetime minority stress, depressive, and PTSD symptoms than SMA living in urban settings. Results from our PMM analysis indicated that heightened experiences of lifetime minority stress indirectly linked the effects of living in rural areas on anxiety (b = –0.288, 95% CI = [–0.491, –0.085]), depressive (b = –0.158, 95% CI = [–0.270, –0.047), and PTSD symptoms PTSD (b = –0.349, 95% CI = [–0.596, –0.105]). The model accounted for 16.8%, 18%, and 24.1% of the variability in anxiety symptoms, depressive symptoms, and PTSD symptoms, respectively.ConclusionsSMA in our study who reside in rural areas reported elevated minority stress, depressive, and PTSD symptoms as compared to their urban dwelling peers. Our study found that lifetime experiences of minority stress fully mediated the relationship between urbanicity and both depressive and PTSD symptoms, and partially mediated the relationship between urbanicity and anxiety. These findings highlight the need to increase support for rural youth who are growing into adulthood and may find continuing challenges in their family, peer, and community relationships.
A 50‐step walking test for analysis of recovery after decompressive surgery for thoracolumbar disc herniation in dogs
Authors: Rosen Suzanne , Grzegorzewski Jessica Lynn , Heath Stephanie , Schocke Cynthia , Jeffery Nicholas 
Published: August 2022

AbstractBackgroundDespite its importance, there is no agreed definition of recovery of ambulation in dogs with spinal cord injury.ObjectivesTo validate a new walking test in dogs recovering from thoracolumbar spinal cord injury.AnimalsTwo hundred twenty‐four dogs weighing <20 kg: 120 normally ambulatory dogs, plus 104 dogs undergoing decompressive surgery for acute thoracolumbar intervertebral disc herniation.MethodsProspective cohort studies. The distance each freely‐ambulatory dog walked during 50 step cycles was regressed on ulna length. For each postsurgical dog, we recorded when the calculated 50‐step distance was completed without falling, or their inability to complete this distance by 4 months or more after surgery. Bayesian analysis compared outcomes for presurgical neurologic categories; association of recovery with several preoperative variables was explored using logistic and time‐to‐event regression.ResultsFor control dogs, 50‐step distance (m) = 1.384 × ulnar length (cm) + 2.773. In postsurgical dogs, the 50‐step test provided decisive evidence that deep pain‐negative dogs were less likely to recover ambulation than dogs with intact pain perception (12/29 recovered vs 71/75; Bayes factor [BF] = 5.9 × 106) and, if they did recover, it took much longer (median 91 days vs median 14 days; BF = 1.5 × 103). Exploratory analysis suggested that presurgical neurologic status (subhazard ratio [SHR] = 0.022; P < .001) and duration of presurgical anesthesia (SHR = 0.740; P = .04) were associated with rapidity of recovery.Conclusions and Clinical ImportanceThis straightforward 50‐step walking test provides robust data on ambulatory recovery well‐suited to large scale pragmatic trials on treatment of thoracolumbar spinal cord injury in dogs.
A detailed record of early solar system melting in the carbonaceous achondrites Northwest Africa 7680 and 6962
Authors: Hyde Brendt C. , Moser Desmond E. , Tait Kimberly T. , Darling James R. , Yin Qing‐Zhu , Sanborn Matthew E. , Banerjee Neil R. , Ali Arshad , Jabeen Iffat , Moreira Hugo 
Published: August 2022

AbstractDetailed textural and geochemical analyses of the carbonaceous achondrites Northwest Africa (NWA) 7680 and NWA 6962 support a rapid progression of thermal events, by similar processes, on the same parent body. The achondrites have olivine compositions of Fa44.8 and Fa47.4 for NWA 7680 and NWA 6962, respectively. Replicate oxygen isotope analyses of grains and bulk powders from NWA 7680 yielded average Δ17O values of −1.04 ± 0.03‰ and −1.00 ± 0.05‰, respectively, which is identical to that reported for NWA 6962. The whole rock ɛ54Cr compositions are also equivalent for NWA 7680 and NWA 6962 (1.36 ± 0.05 and 1.30 ± 0.05, respectively). Both meteorites are plagioclase‐rich, and NWA 7680 is also Fe‐metal‐rich, suggesting they both formed via differentiation processes that resulted in the pooling of partial melt products. Major element geochemical trends show that both rocks could be formed through the melting of chondritic material on a CR chondrite‐like parent body. This is consistent with oxygen isotope and chromium isotope compositions. Intrusion of a late‐stage melt is evident in both meteorites and the crystallization products include silica‐rich, alkali‐deficient nepheline. The late‐stage liquid has partially melted and mixed with primary plagioclase in NWA 6962. In contrast, the late‐stage liquid was often restricted to grain boundaries in NWA 7680, leaving some of the primary plagioclase crystals intact. In situ dating of NWA 7680 phosphate minerals (merrillite and fluorapatite) reveals that it has not experienced long duration thermal metamorphism, or impact‐related Pb loss and age resetting since 4578 ± 17 Ma (207Pb/206Pb age ± 2σ, within error of solar system age). Phosphates associated with the late‐stage melt in NWA 6962 yield a 207Pb/206Pb age of 4556.6 ± 8.0 Ma (2σ) within 2σ of the NWA 7680 age. These early dates indicate that the observed chromium isotope signatures in these meteorites were not introduced by a later high‐temperature event, such as late impact accretion processes. These data are consistent with a rapid separation of inner and outer solar system chemical reservoirs, planetesimal melting, differentiation, and cooling, all within several million years of calcium‐aluminum‐rich inclusion formation.
Automated Magnetic Resonance Image Segmentation of Spinal Structures at the L4‐5 Level with Deep Learning: 3D Reconstruction of Lumbar Intervertebral Foramen
Authors: Chen Tao , Su Zhi‐hai , Liu Zheng , Wang Min , Cui Zhi‐fei , Zhao Lei , Yang Lian‐jun , Zhang Wei‐cong , Liu Xiang , Liu Jin , Tan Shu‐yuan , Li Shao‐lin , Feng Qian‐jin , Pang Shu‐mao , Lu Hai 
Published: August 2022

Objective3D reconstruction of lumbar intervertebral foramen (LIVF) has been beneficial in evaluating surgical trajectory. Still, the current methods of reconstructing the 3D LIVF model are mainly based on manual segmentation, which is laborious and time‐consuming. This study aims to explore the feasibility of automatically segmenting lumbar spinal structures and increasing the speed and accuracy of 3D lumbar intervertebral foramen (LIVF) reconstruction on magnetic resonance image (MRI) at the L4‐5 level.MethodsA total of 100 participants (mean age: 42.2 ± 14.0 years; 52 males and 48 females; mean body mass index, 22.7 ± 3.2 kg/m2), were enrolled in this prospective study between March and July 2020. All participants were scanned on L4‐5 level with a 3T MR unit using 3D T2‐weighted sampling perfection with application‐optimized contrast with various flip‐angle evolutions (SPACE) sequences. The lumbar spine's vertebra bone structures (VBS) and intervertebral discs (IVD) were manually segmented by skilled surgeons according to their anatomical outlines from MRI. Then all manual segmentation were saved and used for training. An automated segmentation method based on a 3D U‐shaped architecture network (3D‐UNet) was introduced for the automated segmentation of lumbar spinal structures. A number of quantitative metrics, including dice similarity coefficient (DSC), precision, and recall, were used to evaluate the performance of the automated segmentation method on MRI. Wilcoxon signed‐rank test was applied to compare morphometric parameters, including foraminal area, height and width of 3D LIVF models between automatic and manual segmentation. The intra‐class correlation coefficient was used to assess the test‐retest reliability and inter‐observer reliability of multiple measurements for these morphometric parameters of 3D LIVF models.ResultsThe automatic segmentation performance of all spinal structures (VBS and IVD) was found to be 0.918 (healthy levels: 0.922; unhealthy levels: 0.916) for the mean DSC, 0.922 (healthy levels: 0.927; unhealthy levels: 0.920) for the mean precision, and 0.917 (healthy levels: 0.918; unhealthy levels: 0.917) for the mean recall in the test dataset. It took approximately 2.5 s to achieve each automated segmentation, far less than the 240 min for manual segmentation. Furthermore, no significant differences were observed in the foraminal area, height and width of the 3D LIVF models between manual and automatic segmentation images (P > 0.05).ConclusionA method of automated MRI segmentation based on deep learning algorithms was capable of rapidly generating accurate segmentation of spinal structures and can be used to construct 3D LIVF models from MRI at the L4‐5 level.
S/F and ROX indices in predicting failure of high‐flow nasal cannula in children
Authors: Kim Ji Hye , Suh Dong In , Park June Dong 
Published: August 2022

AbstractBackgroundA high‐flow nasal cannula (HFNC) is a useful respiratory support for children with respiratory distress; however, it elevates the risk of belated intubation. Recently, indices based on percutaneous oxygen saturation, a fraction of inspired oxygen, and respiratory rate (RR) have been suggested for the prediction of HFNC failure. We aimed to evaluate various indices predicting HFNC failure in children who started receiving HFNC at a tertiary center for 27 months.MethodsCases of HFNC failure were classified as hypoxic respiratory failure (HRF) or non‐HRF (NHRF) depending on the cause of intubation. The ratio of percutaneous oxygen saturation to the fraction of inspired oxygen (S/F), the ratio of S/F to RR (ROX), the ratio of S/F to RR/median RR (ROX‐M), and the ratio of S/F to z‐score of RR (ROX‐Z) were calculated and compared between groups.ResultsOf the 152 cases, 45 (29.6%) failed to wean from the HFNC support, of which 21 (46.7%) were HRFs and 24 (53.3%) were NHRFs. The S/F and ROX‐M at 6 h and 3 h, respectively, predicted HRF well with a high area under the curve. Initial hypercapnia and low weight were good predictors for NHRF.ConclusionsFor the management of children with HFNC, these risk factors and indicators should be monitored to make an early decision regarding intubation.
Role of HLA typing and chimerism analysis in post‐liver transplantation GVHD in pediatrics: A systematic review
Authors: Ghandorah Salim , Alsawaf Baraah , Alrefai Weaam , Ghazwani Aisha , Almusabi Saleh 
Published: August 2022

AbstractBackgroundGVHD is a well‐documented complication after liver transplantation. GVHD occurs when donor immune cells mount a destructive immune response against host cells. The rarity of the GVHD complication and the nonspecific presentation of symptoms and histopathological features provide a diagnostic challenge. Therefore, diagnosis and initiation of treatment are often delayed.AimIn this systematic review, we assessed relevant literature to better understand the utilization of HLA typing and chimerism analysis in liver transplantation. We mainly focused on their importance in diagnosing GVHD incidence after liver transplantation.ResultsA total of 18 articles reported 21 cases of GVHD after liver transplantation in pediatrics. Generally, there is a consensus on the advantage of HLA typing and chimerism analysis in confirming the diagnosis of GVHD after liver transplantation. However, there is an inconsistency in the timing and the application of the accurate HLA typing and chimerism analysis.ConclusionFurther studies are required to assess the incidence of GVHD post‐LT and to determine the impact of HLA typing and chimerism analysis in assessing the risk, early determination of GVHD incidence, and improving outcomes. This systematic review highlights the gap in the field of liver transplantation and calls for revisiting the guidelines to consider HLA typing and chimerism analysis in predicting GVHD before transplantation and diagnosing GVHD incidence after liver transplantation.
Effect of different concentrations of melatonin on ram epididymal spermatozoa recovered post‐mortem under oxidative stress conditions and storage at 4°C
Authors: Shayestehyekta Mohsen , Mohammadi Tayebeh , Soltani Leila , PooyanMehr Mehrdad 
Published: August 2022

AbstractThe current study examines the protective effects of different melatonin concentrations on fresh ram epididymis spermatozoa after post‐mortem recovery under normal and oxidative stress conditions and during liquid preservation (4°C) at different times (24, 48 and 72 h). The testes were obtained from a local slaughterhouse during the breeding season. Spermatozoa were isolated from cauda epididymides. In experiment 1, the effects of adding different concentrations of melatonin (0, 15, 60 and 240 μg/ml) under normal and oxidative stress conditions were evaluated. Fifty μM of hydrogen peroxide was used to induce oxidative stress. Also, in experiment 2, the spermatozoa samples were chilled to 4°C and stored for 72 h. Sperm total motility, viability, membrane, DNA integrity and morphological abnormality were evaluated at 0, 24, 48 and 72 h after cooling storage. In experiment 1, melatonin treatment preserved viability increased TAC and SOD activities, and reduced MDA levels compared with control. Also, melatonin reduced the harmful effects of H2O2under induced oxidative stress. In experiment 2, melatonin at concentrations of 15 and 60 g/ml greatly increased sperm viability after 3 days of cold storage. Furthermore, it could have a significant protective effect on the motility of cooled sperm. Melatonin supplementation preserved higher sperm membrane integrity at concentrations of 15 and 60 μg/ml, DNA integrity at a concentration of 15 μg/ml and abnormality at a concentration of 60 μg/ml after 3 days of storage. The results suggest that melatonin can be used to reduce the adverse effects of induced oxidative stress in spermatozoa. Furthermore, ram epididymal spermatozoa could be stored at 4°C for 72 h when treated with melatonin.