bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synchronized Metabolic and Microbial Changes in Light-vented Bulbul during Recent Northward Range Expansion
Authors: Wu Yun , Xiong Ying , Ji Yanzhu , Cheng Yalin , Zhu Qianghui , Jiao Xiaolu , Chang Yongbin , Zhao Na , Lei Fumin , Song Gang 
Published: November 2022

AbstractBackground Endotherms recently expanding to cold environments generally exhibit strong physiological acclimation to sustain high body temperature. During this process, gut microbes likely play a considerable role in host physiological functions, including digestion and thermogenesis. Light-vented bulbul (Pycnonotus sinensis) represents one of such a species. It used to be restricted to the Oriental realm, and expanded its range to the Palearctic during the past few decades. Results We explored the seasonal dynamics of the resting metabolic rate (RMR) and gut microbiota for local and newly colonized populations of light-vented bulbul. Our results showed that light-vented bulbul exhibited physiological changes during northern expansion. Specifically, the RMR was positively correlated with latitude in the winter (p = 0.0011, r = 0.33). Body mass showed a significant positive correlation with latitude in the breeding (p = 0.045, r = 0.27) and wintering seasons (p = 0.012, r = 0.25), corresponding to Bergmann’s rule. Consistently, the gut microbiota showed corresponding variation to the northern cold environments. In the two northern populations, the alpha diversity decreased compared with those of two southern populations. Significant differences were detected in dominant phyla, such as Firmicutes, Bacteroidetes, Proteobacteria and Desulfobacterota in the breeding and wintering seasons (p < 0.05). The core microbiota showed geographic differences in the winter, including the elevated relative abundance of 5 species (Alistipes shahii,Bacteroides stercoris,Bacteroides vulgatus,Parabacteroides merdaeandBacteroides spp.) in northern populations. Finally, to explore the link between microbial communities and host metabolic thermogenesis, we conducted a correlation analysis between microbiota and RMR. We found that more genera were significantly correlated with RMR in the wintering season than that in the breeding season (71 versus 23). Conclusions This study suggested that key microbiota linked with thermogenesis to push lighted-vented bulbul on a path toward the north.
Exposing New Taxonomic Variation with Inflammation – A Murine Model-Specific Genome Database for Gut Microbiome Researchers
Authors: Leleiwi Ikaia , Rodriguez-Ramos Josue , Shaffer Michael , Sabag-Daigle Anice , Kokkinias Katherine , Flynn Rory M , Daly Rebecca A , Kop Linnea FM , Solden Lindsey M , Ahmer Brian M. M. , Borton Mikayla A , Wrighton Kelly C 
Published: November 2022

Abstract
Background
The murine CBA/J mouse model widely supports immunology and enteric pathogen research. This model has illuminated Salmonella interactions with the gut microbiome since pathogen proliferation does not require disruptive pretreatment of the native microbiota, nor does it become systemic, thereby representing an analog to gastroenteritis disease progression in humans. Despite the value to broad research communities, microbiota in CBA/J mice are not represented in current murine microbiome genome catalogs.
Results
Here we present the first microbial and viral genomic catalog of the CBA/J murine gut microbiome. Using fecal microbial communities from untreated and Salmonella-infected, highly inflamed mice, we performed genomic reconstruction to determine the impacts on gut microbiome membership and functional potential. From high depth whole community sequencing (~ 42.4 Gbps/sample), we reconstructed 2,281 bacterial and 4,516 viral draft genomes. Salmonella challenge significantly altered gut membership in CBA/J mice, revealing 30 genera and 98 species that were conditionally rare and unsampled in non-inflamed mice. Additionally, inflamed communities were depleted in microbial genes that modulate host anti-inflammatory pathways and enriched in genes for respiratory energy generation. Our findings suggest decreases in butyrate concentrations during Salmonella infection corresponded to reductions in the relative abundance in members of the Alistipes. Strain-level comparison of CBA/J microbial genomes to prominent murine gut microbiome databases identified newly sampled lineages in this resource, while comparisons to human gut microbiomes extended the host relevance of dominant CBA/J inflammation resistant strains.
Conclusions
This CBA/J microbiome database provides the first genomic sampling of relevant, uncultivated microorganisms within the gut from this widely used laboratory model. Using this resource, we curated a functional, strain-resolved view on how Salmonella remodels intact murine gut communities, advancing pathobiome understanding beyond inferences from prior amplicon-based approaches. Salmonella-induced inflammation suppressed Alistipes and other dominant members, while rarer commensals like Lactobacillus and Enterococcus endure. The rare and novel species sampled across this inflammation gradient advance the utility of this microbiome resource to benefit the broad research needs of the CBA/J scientific community, and those using murine models for understanding the impact of inflammation on the gut microbiome more generally.
Rapid and robust analytical method for the determination of copper content in commercial pesticides and antifouling biocides
Authors: Pavlidis George , Karasali Helen , Balayiannis George P. 
Published: November 2022

Abstract
The quality control of the agrochemicals and biocidal products in the market requires valid determination methods for the active ingredient content and is of utmost interest to ensure environmental protection, human health, and successful pest control. Copper has been used as fungicide for centuries and today is still in the market in hundreds of products for various uses and is applied in very high application rates, both in pesticides and biocides. A new analytical method has been developed and is proposed as it fast, efficient, and economical for the determination of copper content in antifouling Product Type 21 (PT-21) biocidal products as well as in copper containing pesticides. The samples were oxidized by microwave-assisted acid digestion method and the concentration of copper was determined by flame atomic absorption spectrometry technique. The method performance presented high recoveries, low relative standard deviation, and sufficient Limit of Quantification (LOQ) for the scope of the present analysis. As a result, it is concluded that the method is easily applicable and transferable, with reasonable consumption of reagents, characterized by reliability and sensitivity; therefore, it is suitable for monitoring the levels of copper in antifouling products as well as pesticides containing copper as active substance. The recoveries of the method ranged from 87.9% to 97.6% for antifouling paints and 98.6% to 99.95% for pesticides, while the percentage Relative Standard Deviation (%RSD) was lower than 6% in all cases. The validated method Limit of Quantification (LOQ) was 5 μg ml-1 that was sufficient for the present analysis needs. As a result, it is concluded that the method is easy, with reasonable consumption of reagents, characterized by reliability and sensitivity; therefore, it is suitable for monitoring the levels of copper in antifouling products as well as pesticides containing copper as active substance.
Entropy based approach to manipulator performance measures
Authors: Tarokh Mahmoud , Managadzhe Zaza 
Published: November 2022

Abstract
The paper introduces the new concept of kinematics entropy and utilizes it for performance measures of manipulators. Kinematics entropy is used in a probabilistic framework to propose global measures for the performance of manipulators, which are then specialized to local performance measures. Certain properties of the Jacobian matrix are derived to enable dimensional and unit consistent measures for manipulability. New local manipulability and condition number are proposed that are based on entropy. Examples of a redundant and a non-redundant manipulator are presented to demonstrate the concepts and the usefulness of the measures introduced in the paper in comparison with conventional performance measures
The effect of intraoperative goal-directed fluid therapy combined with enhanced recovery after surgery program on postoperative complications in elderly patients undergoing thoracoscopic pulmonary resection: a prospective randomized controlled study
Authors: Ma Hongmei , Li Xin , Wang Zhe , Qiao Qiao , Gao Yanfeng , Yuan Hui , Guan Bin , Guan Zheng 
Published: November 2022

Abstract
Background: To investigate the effect of intraoperative goal-directed fluid therapy (GDFT) combined with enhanced recovery after surgery (ERAS) program on postoperative complications in elderly patients undergoing thoracoscopic pulmonary resection.
Methods: Patients, more than 60-year-old, undergoing thoracoscopic pulmonary resection for non small cell lung cancer were randomly divided into GDFT group and restrictive fluid therapy (RFT) group. ERAS program was implemented in all patients. In GDFT group, the intraoperative fluid management was guided by stroke volume variation (SVV), cardiac index (CI) and mean arterial pressure (MAP), maintained the SVV<13%, CI>2.5L/min/m2, and MAP>65 mmHg. In RFT group, fluid maintenance with 2 ml/kg/h of balanced crystalloid solution, norepinephrine was used to maintain MAP>65 mmHg. The incidence of postoperative acute kidney injury (AKI), pulmonary and cardiac complications were compared.
Results: 276 patients were enrolled and randomly divided into two groups (138 in each group). Compared to RFT group, the total intraoperative infusion volume, colloids infusion volume, and urine output were larger, the dosage of norepinephrine was lower in GDFT group. Although there were no significant differences of postoperative AKI (GDFT vs RFT; 4.3% vs 8%; P=0.317) and composite postoperative complications (GDFT vs RFT; 66 vs 70) between groups, but the postoperative increase degree of serum creatinine was lower in GDFT group than that in RFT group (GDFT vs RFT; 91.9±25.2 μmol/L vs 97.1±17.6 μmol/L; P=0.048).
Conclusions: Compared to ERAS program with RFT, ERAS program with GDFT has slight beneficial effect on postoperative renal function in elderly patients undergoing thoracoscopic pulmonary resection.
Trial registration: Registered at ClinicalTrials.gov, NCT04302467 on 26 February 2020.
Langerhans Cell Histiocytosis: A Complicated Case Report and Literature Review
Authors: Zhong Xiaoxiao , Shen Jiahui , Ding Fengjiao , Qian Liyuan , Wu Wei , Wen Yanguang , Zhao Yujun , Ding Boni 
Published: November 2022

Abstract
Background: Langerhans cell histiocytosis (LCH) is a rare disease of unknown etiology, characterized by monoclonal proliferation and organ infiltration of Langerhans cells. The clinical manifestations are various, and may involve single or multiple organs. A case of LCH is introduced and the diagnosis and treatment are discussed.
Case presentation: A 23-year-old woman presented with a huge mass in the left lower abdomen. Computed tomography (CT) of the lung revealed multiple diffuse nodules of different sizes in the right lung. The left thorax was collapsed, the left pleura was thickened and adhered, and a small, encapsulated effusion was observed in the thoracic cavity. Enhanced CT of the whole abdomen showed a huge cystic low-density focus on the left lower abdomen, multiple nodules in the spleen, and multiple osteolytic bone destruction in the spine, thorax, and pelvis. The possibility of LCH was considered based on the presence of diffuse reticulum nodules in the lungs observed in CT imaging. The patient was performed ultrasound-guided abdominal puncture, drainage, and chemotherapy with "vinorelbine and cisplatin". Follow-up is ongoing.
Conclusion: LCH involving the serosal membrane is rare. The final diagnosis requires histopathological examination of the involved tissue. But in most cases, it’s enough to diagnose through the typical imaging changes in the lung, as observed by high-resolution computed tomography (HRCT), combined with certain clinical observations. Currently, there are no clearly established treatment guidelines for LCH. At present, systemic chemotherapy is the main treatment method that has been shown to improve patient’s prognosis.
Does value-added tax reform increase the sophistication of enterprise exports? Evidence from China
Authors: Guo Xu , Lin Yiwei , Sun Xiaohua 
Published: November 2022

Abstract
Value-added tax (VAT) is a neutral tax widely adopted around the world that is conducive to easing the tax burden of central and local governments and promoting enterprise innovation. Using the micro-data of China's industrial enterprises from 2000 to 2007,this paper takes the VAT reform in Northeast China as a quasi-natural experiment to explore the impact of reform on the export sophistication of enterprises. This study reveals that the VAT reform increases the sophistication of enterprises' export, and the influence of reform on the export sophistication is found to be insignificant not only for state-owned enterprises but also for medium and low-tech sectors’ enterprises. Moreover, the mechanism analysis shows that the VAT reform stimulates the vitality of enterprise innovation and increases investment in R&D, thereby increasing the sophistication of exported products. The research in this paper is helpful not only for deepening our understanding of the significance of VAT reform but also for further improving policy measures to promote the high-quality development of export trade.
JEL:H25,P25,D22
Stability in gene expression and body-plan development leads to evolutionary conservation
Authors: Uchida Yui , Takeda Hiroyuki , Furusawa Chikara , Irie Naoki 
Published: November 2022

Abstract

Background:
Phenotypic evolution is mainly explained by selection for phenotypic variation arising from factors including mutation and environmental noise. Recent theoretical and experimental studies in the medaka fish Oryzias latipes suggest that greater developmental stability in phenotype and gene expression level, observed under conditions of minimal mutation and environmental noise, is positively correlated with intra-specific evolutionary conservation. Therefore, stability would be a novel intrinsic factor that potentially limits phenotypic variation, promoting evolutionary conservation. However, while these prior studies have revealed correlative relationships between stability and evolutionary conservation, the issue of causality has remained unclear. Here, in Japanese medaka, we tested experimentally whether greater stability in phenotype and gene expression leads to evolutionary conservation.
Results:
Experimental evolution was performed by crossing distantly related Japanese medaka lines, followed by two rounds of intra-generational crossing. We then analyzed whether more stable developmental stages and genes with more stable expression in the F0 generation were more conserved in the F3 generation. The results indicated that greater stability in the F0 generation led to less diversity in the F3 generation.
Conclusions:
These findings reveal that stability in phenotype and gene expression leads to theirevolutionary conservation, most likely by reducing the potential for phenotypic variation. Further, among the developmental stages, the body-plan-establishment stage exhibited the greatest stability and conservation, potentially contributing to the strict conservation of animal body plan.
Fate and cost effectiveness of soil carbon sequestered using supplementary nutrients applied to crop residues under field conditions
Authors: Kirkegaard John A. , Richardson Alan , Kirkby Clive A. 
Published: November 2022

Abstract
Changes in carbon (C) and nitrogen (N) associated with fine fraction (FF) soil were determined to a depth of 180 cm at a field site that was managed with supplementary nutrients (2007–2012) to sequester soil C from crop residues. Soil C and N stocks were monitored for a further 3 years (to 2015) with supplementary nutrients maintained, and then for 5-years after they were ceased (2015–2020). The increase in FF-C established in the first phase of nutrient addition (9.6 t C ha− 1) was maintained during the ongoing 3-yr period of nutrient addition (9.8 t C ha− 1) but declined to 3.0 t C ha− 1 during the subsequent 5-years when nutrient addition ceased. The decline in FF-C stocks was attributed to nutrient mining through increased N mineralization because of insufficient N being applied to fully satisfy the N removed in crops. The addition of supplementary nutrients to the residue influenced crop yield and seed protein in some years, but the responses were crop and season dependant and there was no overall difference in the amount of C in crop biomass returned to the soil. An economic analysis using long-term fertiliser and grain prices suggested that the strategy to sequester C in FF-SOM through nutrient addition to residue was essentially cost neutral. Better tailoring of nutrient inputs to achieve the required stoichiometric ratios in SOM, and an assumed payment for the sequestered C (AU$40 t− 1 CO2 equivalent) provided a beneficial economic outcome during the period of nutrient addition. However the economic benefit was lost when nutrient addition ceased, and losses were significant if fertiliser prices increased or the C price declined. Our results confirm that addition of supplementary nutrients to C-rich crop residues is an effective means to sequester soil C that can persist for several years even after nutrient addition ceases. However, ongoing nutrient inputs in excess of crop removal are required to ensure long-term sequestration of the C from crop residue.
PENGARUH MODAL USAHA, JAM KERJA DAN LAMA USAHA TERHADAP PENDAPATAN USAHA MIKRO KECIL MENENGAH PEDAGANG PASAR GAMBAR KECAMATAN WONODADI KABUPATEN BLITAR
Authors: Alkumairoh Adinda Fuadilla , Warsitasari Wahyu Dwi 
Published: November 2022

Abstrak: Penelitian ini bertujuan mengetahui pengaruh modal usaha, jam kerja, dan lama usaha terhadap pedagang usaha, mikro, kecil, dan menengah (UMKM) di pasar Gambar Wonodadi Blitar baik secara bersamaan maupun sebagian. Teknik penelitian asosiatif dan kuantitatif yang digunakan dalam penelitian ini. Rumus slovin dipakai dengan teknik sampel penelitian, dan total 80 tanggapan responden dianalisis. Uji multikolinearitas, analisis regresi berganda, dan uji asumsi klasik digunakan untuk menganalisis data dalam penelitian ini. Hasil penelitian menunjukkan bahwa variabel modal usaha tidak memiliki dampak positif dan signifikan terhadap pendapatan pedagang mikro, kecil dan menengah (UMKM) pedagang pasar Gambar. Sementara variabel jam kerja dan lama usaha memiliki dampak menguntungkan yang besar pada pendapatan usaha, mikro, kecil dan menengah (UMKM) pedagang pasar Gambar. Jadi besarnya modal belum tentu berdampak pada meningkatnya pendapatan sementara lamanya waktu yang diberikan pedagan berjualan dan lamanya pedagang menekuni usahanya sangat berpengaruh pada pendapatan. Pendapatan usaha mikro, kecil dan menengah (UMKM) pedagang pasar Gambar secara simultan dipengaruhi positif signifikan oleh modal usaha, jam kerja, dan lama usaha.
Kata Kunci: Pendapatan; modal usaha; jam kerja; lama usaha.

Abstract: This study aims to determine the effect of working capital, working hours, and length of business on business traders, micro, small and medium enterprises (MSMEs) in the Gambar Wonodadi Blitar market either simultaneously or partially. Associative and quantitative research techniques are used in this study. Slovin's formula was used with the research sample technique, and a total of 80 respondents' responses were analyzed. Multicollinearity test, multiple regression analysis, and classical assumption test were used to analyze the data in this study. The results showed that the variable of venture capital did not have a positive and significant impact on the income of micro, small and medium traders (MSMEs) of market traders. While the variables of working hours and length of business have a large beneficial impact on the income of business, micro, small and medium enterprises (MSMEs) of market traders. So the amount of capital does not necessarily have an impact on increasing income while the length of time spent by merchants selling and the length of time traders pursue their business greatly affect income. The income of micro, small and medium enterprises (MSMEs) of market traders is simultaneously significantly influenced by business capital, working hours, and length of business.
Keywords: Income; working capital; working hours; same business.
Impact of climate change on spatiotemporal variations in the vegetation cover and hydrology of district Nowshera
Authors: Ali Shah Ishaq , Muhammad Zahir , Khan Haroon 
Published: November 2022

Abstract
This research seeks to validate climate change in the district of Nowshera, as well as its impact on NDVI (vegetation) and hydrological events such as monsoon rains. Modified Mann-Kendall and Sen's statistics were used to examine climate parameters. Using climate factors and MODIS NDVI (Normalized Difference Vegetation Index) data, regression analysis was used to study a 20-year (2001–2020) spatiotemporal climate-vegetation relationship. Landsat 5 and 7 NDWI (Normalized Difference Water Index) data, as well as SRTM DEM (Digital Elevation Model) data, were used to map extreme weather events such as floods caused by climate change. For mapping and data processing, ArcGIS and R tools were used. The findings revealed significant trends in climate parameters, which has led to climate change in the area, affecting NDVI and water cycles. The NDVI showed a generally good trend, however some places were damaged. Monsoon rain patterns and rates have been substantially disrupted, resulting in flash and fluvial floods. The risk of future floods owing to the Kabul and Indus rivers was shown through watershed delineation, which is compounded by climate change and anthropogenic activities. It is advised that government officials and policymakers establish climate change mitigation plans for regional sustainable development and vegetation restoration.