bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bone morphogenetic protein 2/7 promotes repair of bone defect via induction of endochondral ossification
Authors: Wu Jianjun , Zheng Changkun , Liang Guiqing 
Published: January 2022

Purpose: To study the influence of bone morphogenetic protein 2/7 (BMP2/7) on repair of bone defect, and the mechanism involved.Methods: Bone marrow stromal cells (BMSCs) were randomly assigned to 2 groups: control and transfection groups. The cells were transfected with rBMP2/7 recombinant adenovirus. Cell growth and alkaline phosphatase (ALP) activity were determined in both groups. Rabbit model of femoral bone defect was prepared using standard methods. Male New Zealand white rabbits were randomly assigned to 3 groups, each of which had 10 rabbits: control, gelatin and BMP2/7 groups. Histopathological and xray examinations, and three-point bending flexural test were used to compare the potential of gelatin and BMP2/7 to repair bone defects.Results: Transfection of BMSCs with rBMP2/7 recombinant adenovirus significantly enhanced their growth (p < 0.05). Alkaline phosphatase (ALP) level was also markedly and time-dependently higher in transfection group than in control group (p < 0.05). Rabbits with grade 4 bone healing or above were more in BMP2/7-treated category than in control and gelatin groups. New bone hyperplasia with typical lamellar bone structure, irregular medullary cavity, as well as transition from osteoblast to osteocyte were observed in BMP2/7 group. Moreover, maximum flexural strength and repair were significantly higher in BMP2/7-transfected group than in control.Conclusion: These findings indicate that BMP2/7 promotes the repair of bone defect via induction of endochondral ossification in rabbits. Thus, this protein may be useful for the repair of bone defects in humans.
Keywords: Alkaline phosphatase, Bone lesions, BMP2/7, Endochondral ossification
Effect of dexamethasone on levels of inflammatory factors and EGF mRNA in rabbits suffering from oral ulcers
Authors: Wu Weiliang , Ruan Jianyong , Li Daxu , Tao Hong , Deng Chunni , Wang Rui , Cheng Wei , Zhang Zhe , Ren Le 
Published: January 2022

Purpose: To investigate the therapeutic effect of dexamethasone on rabbits suffering from oral ulcers, and the underlying mechanism(s) of action.
Methods: A rabbit model of oral ulcer was established by applying 40 % glacial acetic acid solution to the oral buccal membranes of the animals. Three groups of rabbits were used. Changes in area of the oral ulcer were recorded after dexamethasone administration. Levels of epidermal growth factor (EGF) were assayed using reverse transcription PCR (RT-PCR), while MDA levels and expression levels of IL- 6, IL-8 and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA). Local histopathological changes were examined histologically with the aid of hematoxylin and eosin (H & E) staining.
Results: There were reductions in ulcer areas in group C on the 2nd, 4th and 7th days of dexamethasone administration, when compared with group B (p < 0.05). The EGF levels in the buccal mucosa of rabbits in groups B and C were significantly higher than those in group A (p < 0.05), while the highest EGF level was in group C (p < 0.05). The levels of MDA, IL-6, IL-8, and TNF-α significantly increased in groups B and C (p < 0.05). Results from H & E staining showed lower levels of inflammatory cells in group C than in group B, with visible proliferation of fibroblast cells and epithelial cells in group C after dexamethasone administration.
Conclusion: Dexamethasone accelerates healing of oral ulcer by regulating EGF levels. This finding provides a new approach to the treatment of oral ulcers.
Azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member C1
Authors: You Zhen , Li Bei , Gao Jun , Lu Jiong , Xu Ruihua 
Published: January 2022

Purpose: To investigate the effect of azaindole on proliferation of liver cancer cells, as well as the underlying mechanism.
Methods: Colony forming and 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) assays were used to determine the effect of azaindole on cell proliferation. A tumor model was established through subcutaneous administration of HEPG2 cells to rats. Thereafter, in vivo tumor development was measured using Vernier caliper.
Results: The proliferation potential of HEPG2 and SNU-398 cells was markedly and dose-dependently suppressed by treatment with azaindole at doses of 2, 4, 8, 16 and 20 μM (p < 0.05). The expression levels of Ki67 and PCNA levels were significantly down-regulated in HEPG2 and SNU-398 cells on treatment with 20 μM azaindole. Moreover, azaindole significantly suppressed mRNA and protein expressions of KIFC1 in HEPG2 and SNU-398 cells (p < 0.05). Tumor volume in azaindole-treated rats on day 21 was greatly reduced, while KIFC1 expression in azaindole-treated rat tumor tissue was significantly down-regulated, when compared to the model group (p < 0.05).
Conclusion: Azaindole targets proliferation of liver cancer cells in vitro and inhibits tumor growth in vivo through a mechanism involving down-regulation of KIFCI expression. Thus, azaindole is a potential therapeutic candidate for liver cancer.
Determination of the heavy metal contents of frequently used herbal products in Pakistan
Authors: Quds Tehseen , Ahmed Maryam , Shakeel Sadia , Jalbani Nusrat , Mazhar Farah , Azhar Iqbal 
Published: January 2022

Purpose: To determine the heavy metal content of selected local and international herbal medicines sold for the treatment of various diseases in Pakistan.
Methods: The different dosage forms of herbal medicines assessed were crude forms of syrups, gel, capsule, powder and tonic. Wet digestion method was used to prepare the herbal samples using nitric acid, and then analyzed for arsenic (As), cadmium (Cd), lead (Pb) and mercury (Hg), using MHS-15 mercury/hydride system and flame atomic absorption spectrometry (FAAS).
Results: The investigated results displayed the Arsenic level (0.00 ppm to 0.580 ppm); Cadmium (0.001 ppm to 0.006 ppm); Lead (0.00 ppm to 1.078 ppm) and Mercury (0.001 ppm to 0.012 ppm). All results were found below the permissible limit of acceptability intake of the World Health Organization (WHO) and American Herbal Products Association (AHPA). The pH of the samples were in the range of 1.52 to 6.99.
Conclusion: The findings reveal that the investigated herbal products available in Pakistan are safe with reference to heavy metals, and considered non-toxic for human consumption.
Effect of general and sub-arachnoid anesthesia on the incidence of postoperative delirium and cognitive impairments in elderly Chinese patients
Authors: Ren Wei-Xia , Wu Ran-Ran 
Published: January 2022

Purpose: To investigate the effect of general and subarachnoid (spinal) anesthesia on the incidence of postoperative delirium and cognitive impairments in elderly Chinese patients.
Methods: Elderly Chinese patients (n = 281) aged 65 – 79 years (mean age = 74.12 ± 4.15 years) who underwent proximal femoral fracture surgery were recruited over a 1-year period for this study. The patients were evaluated using neuropsychological assessment battery (NAB) 24 h before surgery, and on the first day 1 month after surgery. Data on activity of daily living (ADL) (in this case toileting at the time of discharge) were recorded and analyzed.
Results: There was no significant difference in the number of patients that developed postoperative delirium between the two anesthesia groups (p > 0.05). Although the trail making test (TMT) scores (parts A and B) were increased on the first day 1 month after surgery, there were no significant differences in NAB results between the two groups (p > 0.05). Patients who received subarachnoid (spinal) anesthesia had significantly higher dependency for toileting at the time of discharge than those who received general anesthesia (p < 0.05).
Conclusion: These results show that general and subarachnoid (spinal) anesthesia do not cause postoperative delirium and cognitive dysfunction in elderly Chinese patients who underwent proximal femoral fracture surgery.
Protective effect of syringaresinol on rats with diabetic nephropathy via regulation of Nrf2/HO-1 and TGF- β1/Smads pathways
Authors: Ji Lei , Zhong Xue , Xia Xingxing , Yu Wei , Qin Yuping 
Published: January 2022

Purpose: To investigate the protective role of syringaresinol in a rat model of diabetic nephropathy (DN).
Methods: Streptozotocin was injected intraperitoneally into rats to establish the diabetic model. Streptozotocin-induced rats were orally administered syringaresinol, and pathological changes in kidneys were assessed using hematoxylin and eosin staining. Enzyme-linked immunosorbent assay (ELISA) was used to determine kidney injury indicators, 24-h urine proteins, blood urea nitrogen (BUN), and serum creatinine (SCR). Blood glucose was measured using a blood glucose meter, while levels of malonaldehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX) in kidney were also measured using ELISA.
Results: Pathological changes in the kidneys were observed in rats post-streptozotocin treatment. Administration of syringaresinol reduced the lesion degree, with improved pathological morphology in kidney. Syringaresinol administration significantly attenuated streptozotocin-increased levels of BUN, SCR, 24-h urine protein, and blood glucose (p < 0.01). Streptozotocin-induced oxidative stress, shown by enhanced MDA level and reduced levels of SOD, CAT, and GSH-PX, was reversed in rat kidneys following syringaresinol administration. However, the expression levels of nuclear factor erythropoietin- 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) proteins decreased, while transforming growth factor-beta 1 (TGF-β1) and signal transducer and transcriptional modulator (Smad) 2/3/7 proteins increased in rats post-streptozotocin treatment. Syringaresinol administration reversed the effects of streptozotocin on protein expression of Nrf2, HO-1, TGF-β1, and Smad 2/3/7.
Conclusion: Syringaresinol exerted a protective effect against DN through activation of Nrf2 and inactivation of TGF-β1/Smad pathways. Thus, the compound can potentially be developed for management of diabetic nephropathy.
Sarcoidosis – a multisystem disease
Authors: Franzen Daniel P. , Brutsche Martin , Nilsson Jakob , Böni Christian , Daccord Cécile , Distler Oliver , Elsener Dieter , Funke-Chambour Manuela , Gruner Christiane , Hayward-Könnecke Helen , Hostettler Katrin E. , Kündig Thomas , Ribi Camillo , Seebach Jörg D. , Seeger Harald , Vrugt Bart , Kolios Antonios G. A. 
Published: January 2022

Sarcoidosis is a systemic inflammatory disease, characterised by granuloma formation upon an unknown trigger in genetically predisposed individuals. The inflammation is characterised by an activation of both the innate immune system, with macrophages differentiating into epitheloid cells and dendritic cells, and the adaptive immune system, particularly T helper (Th) 1 and Th17 cells. Since all organs can be affected to varying extents, clinical presentation is often diverse. Most commonly, the lungs, lymph nodes, skin and eyes are involved, whereas cardiac, renal and neurological manifestations are less common but associated with higher morbidity. Depending on the clinical symptoms, a detailed evaluation including thorough clinical examination, imaging and laboratory tests should explore all possible organ involvements. In some patients, fatigue manifests as a para-sarcoidosis symptom impacting quality of life, even if sarcoidosis is in remission.
Some acute syndromic presentations, such as Löfgren’s syndrome, have a good prognosis and are commonly self-limiting. If possible, a topical treatment, for example for cutaneous sarcoidosis or bronchial involvement, should be applied. Treatment of severe cases with persisting disease activity necessitates long-term immunosuppressive drugs, with glucocorticoids as the first-line option. Steroid-sparing and second-line drugs include methotrexate, azathioprine, mycophenolate mofetil and immunomodulators such hydroxychloroquine, with the latter being first-line therapy in cutaneous sarcoidosis. Tumour necrosis factor-alpha inhibitors (particularly adalimumab and infliximab) are used as third-line agents but are administered earlier in cases of persistent disease activity, severe organ-involvement or intolerance to conventional drugs. Treatment decisions should be based on a multidisciplinary approach, depending on organ involvement and treatment tolerability. Para-sarcoidosis manifestations, particularly fatigue, should also be carefully addressed, where the patient could also be enrolled in multidimensional rehabilitation programmes.
With various organ involvement and different phenotypes, larger studies including real-world data from registries are necessary to evaluate different sarcoidosis endotypes and preferential treatment pathways.
VPLYV TEPLOTY PROSTREDIA NA SPOTREBU ELEKTRICKÝCH AUTOBUSOV
Authors: Čulík Kristián , Hrudkay Karol , Štefancová Vladimíra 
Published: January 2022

Tento príspevok je zameraný na výskum vplyvu okolitej teploty na spotrebu elektrickej energie elektrobusov. Východiskovým podkladom výskumu bola detailná databáza prevádzkových údajov a taktiež záznamy o priemernej teplote v mieste prevádzky elektrobusov. V úvode rozoberáme aj postupný nástup technológie elektrobusov v Slovenskej republike a mapujeme zloženie vozidlového parku. V tomto prípade sme analyzovali vozidlá Škoda Perun 26SH01, pričom sme sa zamerali na zimnú a letnú prevádzku. Analyzované mesiace boli výrazne odlišné priemernou dennou teplotou. Tá má zásadný vplyv na celkovú spotrebu elektrobusu, pretože analyzovaný typ má kabínu cestujúcich vykurovanú práve elektrickými ohrievačmi. V článku odpovedáme na niekoľko výskumných otázok súvisiacich so spotrebou elektrobusov v našich podnebných podmienkach.
INTEGROVANÉ TAKTOVÉ JÍZDNÍ ŘÁDY - ČÁST I
Authors: Hlinovský Jakub 
Published: January 2022

Tento odborný článek seznámí čtenáře s prvopočátky zavedení Integrovaných taktových jízdních řádů a samozřejmě taky se základními pojmy, které se používají při jejich tvorbě. Článek se rovněž bude zmiňovat o matematických výpočtech pro zavádění zastávkových vlaků s úplnou dosažitelností míst zastavení.
DOPRAVA E-VTOL LETADLY V ČESKÉ REPUBLICE – ZNALOSTI A OČEKÁVÁNÍ VEŘEJNOSTI
Authors: Zýka Jan , Čmielová Kristýna , Vosečková Lucie 
Published: January 2022

Letadla se svislým vzletem a přistáním poháněná elektrickými pohonnými jednotkami jsou považována za jednu z hlavních oblastí budoucí vzdušné přepravy osob nebo zboží na kratší vzdálenosti. Článek představuje tento druh dopravy a uvádí jeho přednosti a nedostatky. Hlavní část je poté věnována průzkumu povědomí české veřejnosti o e-VTOL letadlech a poukazuje i na očekávání, která si lidé s novým druhem dopravy spojují. K tomu byla použita zjednodušená metoda kvantitativního výzkumu za pomoci dotazníkového šetření. Bylo zjištěno, že většina veřejnosti nemá o e-VTOL letadlech povědomí, avšak poté, co byla technologie vysvětlena, by ji byli lidé ochotni využít, pokud by cena za přepravu bylo nanejvýš o 20 % vyšší než konvenční druhy (pozemní) dopravy.
UMÍSTĚNÍ SVARU A JEHO VLIV NA KONCENTRACI NAPĚTÍ
Authors: Jilek Petr , Berg Jan 
Published: January 2022

Příspěvek se zabývá umístěním svarů rámu a jejich vlivem na pevnost konstrukce. U konstrukce rámu je nutné brát zřetel na minimalizaci hmotnosti při zachování jeho dostatečné pevnosti a únosnosti. Za tímto účelem byl vytvořen 3D model rámu, který odpovídá reálnému provedení rámu, a tento model byl podroben analýze metodou konečných prvků (MKP). Na základě této analýzy byly vytipovány oblasti s koncentrací napětí. Požadavkem je, aby pokud možno se směr svarů co nejméně vyskytoval v oblastech vysokého mechanického napětí. Díky vhodnému uspořádání svarů může dojít ke snížení hmotnosti rámu díky použití profilů s nižší tloušťkou stěny při zachování požadované pevnosti. Návrhem vhodného směru svarů a kontrolních zkoušek mohl být zvýšen sooučinitel hodnoty svarového spoje v dané oblasti a tím i maximální dovolené namáhání.
ANALISIS PEMANFAATAN MIKROORGANISME LOKAL (MOL) DENGAN VARIASI STANDAR MCFARLAND PADA PENGOLAHAN LIMBAH CAIR TEMPAT PELELANGAN IKAN (TPI) GUSUNG KECAMATAN SAPE KABUPATEN BIMA
Authors: Nurhidayah Nurhidayah , Wahyuningsih Sri 
Published: January 2022

Tempat Pelelangan Ikan (TPI) Gusung merupakan pusat pelelangan ikan yang keberadaannya sangat penting namun aspek sanitasi di dalam TPI tidak terawat, sehingga perlu dilakukan pengolahan terhadap air limbah yang dihasilkan. Tujuan penelitian yaitu untuk menganalisis manfaat MOL sebagai bioactivator pada degradasi limbah cair TPI Gusung dan untuk mengetahui standar McFarland yang optimal dalam pengolahan atau degradasi limbah cair TPI Gusung Kabupaten Bima. Metode yang digunakan yaitu eksperimen dengan menggunakan MOL sebagai starter dengan variasi Mcfarland. Hasil penelitian yaitu pemanfaatan MOL mampu menurunkan pencemar sehingga memenuhi standar baku mutu, dan amoniak adalah parameter penurunan tertinggi pada hari ke-5 dan ke-10 (Mcfarland 0,5, 1 dan 1,5) yaitu <0,062 mg/L, penurunan BOD tertinggi pada akhir pengamatan yaitu pada Mcfarland 1,5 sebesar 1,1 mg/L, dan COD sebesar 24 mg/L pada 1,5 kemudian 31 dan 33 pada Mcfarland 0,5 dan 1. Penurunan minyak lemak (2,92 mg/L ) dan TSS (1,7 mg/L) di akhir pengamatan pada Mcfarland 1,5, dan konsentrasi terendah pada semua parameter yaitu pada bak kontrol. Standar Mcfarland yang optimal dalam mendegradasi limbah cair TPI yaitu 1,5 dengan perkiraan jumlah mikroorganisme sebesar 4,5 x 108 sel/ml, berdasarkan hasil yang diperoleh menunjukkan semakin tinggi Mcfarland (semakin banyak jumlah sel mikroorganisme sebagai starter), maka semakin optimal penurunan konsentrasi pencemar. Saran untuk peneliti selanjutnya agar bisa melakukan identifikasi MOL yang diaplikasikan pada limbah cair TPI hingga tingkat spesies, agar memudahkan dalam proses perbanyakan dan penyimpanan dalam waktu yang cukup lama sebelum diaplikasikan sebagai bioactivator limbah.
Occupational Therapy Service Delivery Among Medicaid-Enrolled Children and Adults on the Autism Spectrum and With Other Intellectual Disabilities
Authors: Benevides Teal W. , Tao Sha , Becker Alec , Verstreate Kate , Shea Lindsay 
Published: January 2022

Abstract
Importance: Rates of occupational therapy service utilization among people with autism spectrum disorder (ASD) or intellectual disability (ID) have not been explored in population-based samples.
Objective: To describe occupational therapy services delivered to Medicaid-eligible persons younger than age 65 yr identified as having ASD, ID, or both and to evaluate demographic factors associated with occupational therapy service utilization in this population.
Design: Retrospective, case–control, cohort study using claims records from Medicaid Analytic eXtract files (2009–2012).
Setting: Data from all 50 states and Washington, DC.
Participants: Beneficiaries identified as having ASD only, ASD+ID, or ID only who were younger than age 18 yr (N = 664,214) and ages 18–64 yr (N = 702,338).
Outcomes and Measures: We analyzed Current Procedural Terminology® and Healthcare Common Procedure Coding System procedure codes, Medicaid Statistical Information System type of service codes, and Center for Medicare & Medicaid Services provider specialty codes.
Results: Only 3.7% to 6.3% of eligible adult beneficiaries received occupational therapy; in contrast, 20.5% to 24.2% of children received occupational therapy. Significant predictors of service use varied by group; however, differences by race–ethnicity, eligibility on the basis of poverty, and geographic location were observed. Among children, the most frequent billing code was for “therapeutic activities” (43%–60%); among adults, it was “community/work reintegration training” (29%–39%).
Conclusions and Relevance: Billed procedure code patterns do not consistently reflect the unique occupational focus that occupational therapy providers deliver to people with developmental disabilities. Disparities in occupational therapy receipt warrant further attention to understand the social and structural factors affecting service delivery.
What This Article Adds: Occupational therapy services paid for by Medicaid are used more frequently by children with ASD and ID than by adults with these diagnoses. Greater understanding of the intersectional factors that drive service delivery and disparities is needed.
Sistem Informasi Administrasi Menggunakan Metode Waterfall Pada Kelurahan Kalidoni Kota Palembang
Authors: Riansyah Riansyah , Ruliansyah Ruliansyah , Rahayu Sri 
Published: January 2022

Proses layanan administrasi di Kelurahan Kalidoni masih dilakukan secara konvensional. Pemohon (penduduk) harus datang sendiri dan dilayani secara manual untuk membuat surat keterangan nikah, surat keterangan tidak mampu, surat keterangan tidak memiliki rumah, surat menikah, surat kematian, surat keterangan usaha, surat KPR rumah, surat izin bangunan, surat pengantar SKCK atau surat keramaian. Penginputan data dan pembuatan layanan surat harus diketik menggunakan aplikasi Microsoft Word dan Microsoft Excel. Pemohon harus datang ke kantor kelurahan Kalidoni untuk membuat dan mengetahui status pengajuan layanan yang diajukannya. Pemohon juga harus bolak balik ke Kantor jika kekurangan syarat untuk pengurusan administrasi sehingga proses pelayanan membutuhkan waktu yang sering kali melebihi standar waktu maksimal yang telah ditetapkan. Kondisi yang sama juga terjadi pada pegawai kelurahan yang mengalami kesulitan dalam memberikan layanan yang maksimal kepada masyarakat. Untuk mengatasi permasalahan tersebut maka dibuatlah sistem informasi administrasi yang berbasis web yang dapat melayani pemohon untuk membuat surat-menyurat. Pembuatan sistem ini menggunakan metode waterfall dengan tahapan analisis kebutuhan, desain sistem, penulisan program, pengujian program, dan penerapan program dan pemeliharaan. Hasil uji implementasi memperlihatkan bahwa sistem yang dibangun dapat membantu pemohon dan pegawai kelurahan dalam proses pembuatan surat-menyurat karena sudah terkomputerisasi sehingga hasil yang didapat lebih cepat dan dapat terdokumentasi lebih baik.
Korelasi Intensitas Bimbingan Pembimbing Akademik Dengan Indeks Prestasi Mahasiswa di STAI Darul Kamal NW Kembang Kerang
Authors: Musabbihin Musabbihin , Yamin Muhammad , Sadaruddin Muhammad , Sauri Saupian 
Published: January 2022

Manusia dalam hal ini adalah mahasiswa/mahasiswi STAI Darul Kamal NW Kembang Kerang Daya. Membutuhkan bantuan berupa pelaksanaan kegiatan bimbingan yang dilakukan secara berkesinambungan agar dapat memahami dirinya sendiri sehingga dapat bertindak sesuai dengan tuntunan dan keadaan lingkungan dalam keluarga, pendidikan, dan masyarakat. Dalam dunia kampus mahasiswa dituntut untuk berkopotensi dalam memperoleh prestasi akademik, dimana tolak ukurnya adalah indeks prestasi. Penelitian ini bertujuan melihat hubungan antara intensitas bimbingan dengan prestasi akademik mahasiswa. penelitian dilakukan dengan memberikan angket kepada mahasiswa STAI darul kamal yang dijadikan sampel. sampel dipilih dengan teknik random sampling dan diapatkan 30 mahasiswa sebagai sempel penelitian. analisis data hasil penelitian dilakukan dengan uji korelasi. Hasil analisis menunjukan bahwa terdapat hubungan yang signifikan antara intensitas bimbingan akademik pada mahasiswa dengan nilai IPK.