bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal evaluation of efficacy and quality of tissue repair upon laser‐activated sealing
Authors: Ghosh Deepanjan , Salinas Christopher M. , Pallod Shubham , Roberts Jordan , Makin Inder Raj S. , Yaron Jordan R. , Witte Russell S. , Rege Kaushal 
Published: September 2022

AbstractInjuries caused by surgical incisions or traumatic lacerations compromise the structural and functional integrity of skin. Immediate approximation and robust repair of skin are critical to minimize occurrences of dehiscence and infection that can lead to impaired healing and further complication. Light‐activated skin sealing has emerged as an alternative to sutures, staples, and superficial adhesives, which do not integrate with tissues and are prone to scarring and infection. Here, we evaluate both shorter‐ and longer‐term efficacy of tissue repair response following laser‐activated sealing of full‐thickness skin incisions in immunocompetent mice and compare them to the efficacy seen with sutures. Laser‐activated sealants (LASEs) in which, indocyanine green was embedded within silk fibroin films, were used to form viscous pastes and applied over wound edges. A hand‐held, near‐infrared laser was applied over the incision, and conversion of the light energy to heat by the LASE facilitated rapid photothermal sealing of the wound in approximately 1 min. Tissue repair with LASEs was evaluated using functional recovery (transepidermal water loss), biomechanical recovery (tensile strength), tissue visualization (ultrasound [US] and photoacoustic imaging [PAI]), and histology, and compared with that seen in sutures. Our studies indicate that LASEs promoted earlier recovery of barrier and mechanical function of healed skin compared to suture‐closed incisions. Visualization of sealed skin using US and PAI indicated integration of the LASE with the tissue. Histological analyses of LASE‐sealed skin sections showed reduced neutrophil and increased proresolution macrophages on Days 2 and 7 postclosure of incisions, without an increase in scarring or fibrosis. Together, our studies show that simple fabrication and application methods combined with rapid sealing of wound edges with improved histological outcomes make LASE a promising alternative for management of incisional wounds and lacerations.
Effect of phytase corn addition on ethanol yield and distillers' dried grains with soluble profile in corn dry‐grind process
Authors: Singh Ramkrishna , Lessard Philip , Michael Raab R. , Singh Vijay 
Published: September 2022

AbstractBackground and ObjectivesPhytase as a feed additive improves the bioavailable phosphorus content of the poultry diet, thereby decreasing dependence on externally added phosphorus. The objective of this study was to determine the effect of adding phytase corn on ethanol yield and distillers' dried grains with soluble (DDGS) composition during a corn dry‐grind process.FindingsPhytase corn at 2.5%, 5%, 7.5%, and 10% w/w of fermentable corn was added during the dry‐grind process. Upon fermentation, the ethanol yield was observed to be in the range of 17.9%–18.9% v/v compared to control (17.7% v/v), representing an 1%–6.5% increase in ethanol yield. Relative to the corn that was introduced at the beginning of the fermentation, the DDGS obtained from the control corn or phytase corn addition all showed a threefold increase in oil, protein, and neutral detergent fiber content. However, the phytic acid content of the DDGS was reduced from 0.45% w/w (control) to 0.13% w/w (phytase treatments).ConclusionAdding phytase corn in a dry‐grind process improves the ethanol yield and reduces phytate‐bound phosphorus content.Significance and NoveltyPhytase corn addition will improve the ethanol yield, DDGS nutritional quality in a corn dry‐grind process, and reduce dependence on phosphorus supplementation.
Denoising stacked autoencoders‐based near‐infrared quality monitoring method via robust samples evaluation
Authors: Lv Jiapeng , Chen Zihao , Luan Xiaoli , Liu Fei 
Published: September 2022

AbstractThis paper proposes a denoising stacked autoencoders‐based near‐infrared spectroscopy on‐line quality monitoring model via robust sample evaluation. The previous related work tends to focus on the near infrared spectrum data from the high dimension, multicollinearity, and information redundancy, and so on, but pays less attention to its inherent nonlinearity and sensitivity caused by internal and external factors (i.e., particle size, colour, moisture, uniformity, temperature, and so on). First, this paper aims to achieve feature extraction in nonlinearity with a denoising stack autoencoder, overcoming the impact of over‐sensitivity described as given distributed noise. Second, we propose a robust sample evaluation method derived from the robust statistics to tell and eliminate the individual samples contrary to the potentially statistical rules learned by the established model from the population samples and retrain the model with a more robust training set. The near‐infrared spectrum data derived from the distillation process of 2, 6 xylenol are used as a case in this paper to verify the validity and accuracy of the monitoring model proposed above.
Recent Advances in Solution‐processed Inorganic Thermoelectric Thin Films
Authors: Hwae Heo Seung , Shin Hosun , Sung Son Jae 
Published: September 2022

AbstractRecent advancements in microscale electronics and wearable devices have led to the concept of energy autonomy, which requires self‐powered energy sources that can be integrated into systems. Thermoelectric generators have received significant attention as direct energy conversion systems between heat and electrical energy. However, typical bulk‐scale inorganic thermoelectric materials have limited utility in these systems, and the traditional fabrication methods of generators pose difficulty in their miniaturization. Thin‐film thermoelectric materials can address these issues owing to their easy integration into microscale systems. Moreover, solution processing can provide a cost‐effective and scalable production approach for inorganic thin films. In this paper, we review the recent progress in solution‐processed inorganic thermoelectric thin films, including the synthesis of ink solutions, fabrication of thin films, and thermoelectric performance of materials and devices.
A Novel Electron Donor‐Acceptor Carbazole‐Zn(II)Phthalocyanine – Perfluorinated Subphthalocyanine Conjugate: Synthesis, Characterization, and Photoinduced Electron‐Transfer
Authors: Platzer Benedikt , Ghazal Basma , Mariñas Víctor , Labella Jorge , Martínez‐Díaz M. Victoria , Maksheed Saad , Guldi Dirk M. , Torres Tomás 
Published: September 2022

AbstractPorphyrinoids are considered perfect candidates for the preparation of model electron donor‐acceptor (D‐A) systems as they enable fast and efficient photoinduced electron transfer. Herein, we report on the synthesis and photophysical characterization of a ZnPc−SubPc conjugate covalently connected through a short‐range alkyne spacer. We designed and prepared the conjugate, which comprise, on the one hand, a perfluorinated SubPc with strong electron acceptor character and, on the other, a Pc peripherally functionalized with carbazoles with strong electron donor character. Photoinduced electron transfer events are in‐depth analyzed by several techniques, including steady‐state absorption, time‐resolved emission and transient absorption measurements on different time scales. Our studies confirm a full charge separation occurring from a photoexcited charge transfer state.
DNA damage in human oral cells induced by use of e‐cigarettes
Authors: Guo Jiehong , Hecht Stephen S. 
Published: September 2022

AbstractThe use of electronic cigarettes (e‐cigarettes) has increased rapidly in the United States, especially among high school students. e‐Cigarettes contain some recognized carcinogens and may induce DNA damage in oral cells. The aim of this review is to summarize studies reporting DNA adducts or other types of DNA damage in oral cells in vitro or in vivo upon exposure to e‐cigarette vapor and to evaluate the possible connections between e‐cigarette exposure and oral cancer. Three databases including PubMed, Scopus, and EMBASE and gray literature were searched for articles published up to April 24, 2022. After screening 321 articles, we extracted 27 for further investigation. Based on the inclusion criteria, 22 articles were eligible for this review. The in vitro studies demonstrate that e‐cigarette liquid or vapor can induce DNA damage, oxidative stress, DNA double‐stranded breaks, apoptosis, cytotoxicity, and genotoxicity in different types of oral cells. The clinical studies showed that e‐cigarette users have significantly higher levels of N′‐nitrosonornicotine, acrolein DNA adducts, metanuclear anomalies, gene regulation, and lactate dehydrogenase enzyme expression and significantly lower levels of apurinic/apyrimidinic sites than non‐users. Comparison of micronuclei levels between e‐cigarette users and non‐users gave inconsistent results. e‐Cigarettes are implicated in DNA damage to oral cells, but publications to date present limited evidence. Future studies with larger sample sizes are required to investigate the long‐term consequences of e‐cigarette use.
Mapping institutional change: Analysing strategies for institutional design in collective infrastructure renewal
Authors: Neef Robin , Busscher Tim , Verweij Stefan , Arts Jos 
Published: September 2022

AbstractActors' toolset to affect institutional change bydoinginstitutional design is limited because criteria for effective institutional design are often too general and abstract. This paper aims to identify institutional design strategies and explore how they influence institutional change. The theoretical framework builds on Ostrom's Institutional Analysis and Development framework to map institutional change, and it identifies six institutional design strategies: framing, puzzling, powering, network composition, network outcomes, and network interaction. A comparative case study on Dutch infrastructure renewal opportunities – one case's institutional design interventions attained collective renewal, the other did not – maps institutional change in decision‐making rounds through institutional directions. Key findings include that institutional change of position, boundary, choice, and information rules first is conducive to collective action. Moreover, mimicry of especially choice rules is pivotal. Furthermore, institutional design strategies have a configurational nature: microlevel strategies have mesolevel consequences, and some configurations instigate change, whereas others cause dynamic inertia.
El Niño, La Niña, and forecastability of the realized variance of agricultural commodity prices: Evidence from a machine learning approach
Authors: Bonato Matteo , Çepni Oğuzhan , Gupta Rangan , Pierdzioch Christian 
Published: September 2022

AbstractWe examine the predictive value of El Niño and La Niña weather episodes for the subsequent realized variance of 16 agricultural commodity prices. To this end, we use high‐frequency data covering the period from 2009 to 2020 to estimate the realized variance along realized skewness, realized kurtosis, realized jumps, and realized upside and downside tail risks as control variables. Accounting for the impact of the control variables as well as spillover effects from the realized variances of the other agricultural commodities in our sample, we estimate an extended heterogeneous autoregressive (HAR) model by means of random forests to capture in a purely data‐driven way potentially nonlinear links between El Niño and La Niña and the subsequent realized variance. We document such nonlinear links, and that El Niño and La Niña increase forecast accuracy, especially at longer forecast horizons, for several of the agricultural commodities that we study in this research.
A university‐led initiative to expand pharmacy residency program development statewide
Authors: Taylor Stacy , Hudspeth Brooke 
Published: September 2022

AbstractGiven trends in pharmacy practice advancement, additional residency training positions are needed to meet future workforce development goals. Although there has been aggressive growth in the number of pharmacy residency training programs over the past decade, a shortage in the number of available residency positions persists. As such, residency expansion initiatives across the profession are ongoing. To describe a university‐led initiative to expand pharmacy residency program development statewide, programmatic outcomes associated with implementation, and key elements of success. Health systems interested in residency program development were invited to participate in a university‐led initiative that included an online residency resource repository, preceptor interest inventory, monthly video calls, collaboratively established goals and deadlines, and preceptor development. Health system participation rates, utilization of resources, and programmatic outcomes such as number of new residency programs and positions were tracked over time. Over a 3‐year period, six health systems participated in the initiative, yielding four new postgraduate year 1 (PGY1) programs and eight new PGY1 positions. Five of six health systems utilized the online resources and participated in the monthly video calls. Four of the six health systems accessed the preceptor development materials. A University‐led initiative is an effective method for developing new residency training programs. Widescale efforts to implement models such as this one, could prove beneficial in addressing the nationwide shortage of residency programs.
Functional morphology of cirri in the barnacle Amphibalanus improvisus (crustacea: Balanidae)
Authors: Hindenberg Lena M. , Gorb Stanislav N. , Büsse Sebastian 
Published: September 2022

AbstractBarnacles rely heavily on their mobile cirri for food capture because of the sessile lifestyle. These filamentous food capturing devices are extended into the water current and perform undulating movements. Cuticular structures with corresponding musculature work together, to allow these highly repetitive movements. This paper studies the interplay between structure and function of the cirri using scanning electron microscopy (SEM), microcomputed tomography (µCT) and high‐speed video recordings (HSV) in Amphibalanus improvisus. Barnacles use the external cuticle structures (denticles and setae) for efficient grooming, food capturing, and providing support for the muscular system responsible for movement control. Entanglement of the cirri during extension is probably avoided through an interlocking of the serrate setae on cirri IV–VI, creating a “zipper‐like” effect, which was recorded here using the HSV. We analyzed the muscular arrangement using µCT and found a new flexor muscle in both the endo‐ and exopod of cirrus II. Supported by the intrinsic cirral muscles, the new flexor muscles may provide variable movements of the anterior cirri (cirri I–III), which is important for further food handling. Our results provide a foundation for further comparative studies of the feeding apparatus of barnacles and for possible implications in the area of bio‐inspired robotics.
Study on the processing chemistry of Fructus Psoraleae by a combination of untargeted and targeted metabolomics
Authors: Mu Liyan , Dai Hui , Fei Chenghao , Li Wenjing , Xue Qianqian , Xu Yan , Li Lin , Li Weidong , Yin Wu , Yin Fangzhou 
Published: September 2022

Fructus Psoralea is widely used to treat osteoporosis and skin inflammatory diseases. Because of the side effects on the liver, renal and cardiovascular systems, it is processed to salt‐processed Fructus Psoraleae to meet the requirements of clinical use. However, the mechanisms involved in the transformation of the chemical components are unclear. In this study, ultra‐high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry was used to analyze the chemical profiles of this herbal medicine and the chemical transformation mechanism involved during the salt processing was studied. A total of 83 compounds were identified. Principal component analysis and orthogonal partial least squares discriminate analysis were used to observe the distribution trend of all samples and visualize the difference. Raw and processed Fructus Psoraleae were clearly clustered into two groups. Furthermore, 17 marker compounds were identified as primary contributors to their differences based on t‐test analysis (p < 0.01) and orthogonal partial least squares discriminate analysis (variable importance for the projection > 1). Finally, ultra‐high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry was used to evaluate the quality of Fructus Psoraleae by simultaneous analysis of 13 components highly related to efficacy. There were variations in the contents of 13 chemicals of Fructus Psoraleae and salt‐processed products. The results of untargeted and targeted metabolomics revealed that salt processing affected the chemical composition of Fructus Psoraleae.
Two heterozygous mutations in the calcium/calmodulin‐dependent serine protein kinase gene (CASK) in cases with developmental disorders
Authors: Yang Kunfang , Lin Longlong , Yuan Fang , Li Xiaoguang , Liu Zhiping , Lan Xiaoping , Wang Yilin , Ren Yun , Li Jiaoyan , Chen Yucai 
Published: September 2022

AbstractBackgroundThe calcium/calmodulin‐dependent serine protein kinase gene (CASK) is an essential gene in mammals, critical for neurodevelopment. The purpose of this study is to expand the understanding of the diagnosis of CASK‐linked disorders.Materials/MethodsFrom clinical and genetic mutational analyses, relevant data in 2 Han Chinese patients were collected and analyzed. Real‐time quantitative PCR (RT‐qPCR) was performed to investigate the CASK expression levels in the patients. The X‐chromosome inactivation (XCI) patterns of the patients and their nuclear families were tested by quantitation of methylation of the polymorphic human androgen receptor (HUMARA) locus.ResultsTwo Han Chinese patients both presented with intellectual disability (ID), microcephaly with pontine and cerebellar hypoplasia (MICPCH). Two de novo mutations of c.82C>T (p.Arg28*) and c.846C>G (p.Tyr282*) in CASK have been investigated and predicted to be deleterious, which have produced truncated proteins. The functional protein association network of STRING (http://string‐db.org) generated three‐dimensional (3D) atomic models based on protein sequences in CASK and two Arg28 and Tyr282 residues were marked. RT‐qPCR showed lower copy numbers of CASK expression in the patients than in their parents, as well as the sex‐ and age‐ matched control groups. Patient 1 showed a skewed XCI pattern, while no related changes noted in patient 2.ConclusionsPatients carrying different nonsense variants may have different degrees of different clinical phenotypes. This study expands the spectrum of genotype and phenotype correlations of CASK‐linked disorders in the Han Chinese ethnicity and provides new insights into the molecular mechanism.
Fabrication and post‐processing of PI/PVDF‐HFP/PI electrospun sandwich separators for lithium‐ion batteries
Authors: Parsaei Solmaz , Zebarjad Seyed Mojtaba , Moghim Mohammad Hadi 
Published: September 2022

AbstractPI/PVDF‐HFP/PI sandwich separators were prepared by electrospinning, and subsequent pressing and heat treatment were used to improve their properties. Morphology, porosity, air permeability, contact angle, and other specifications of the separators were evaluated. The results showed that heat treatment changed separator porosity and permeability. The pressed and heat‐treated PI/PVDF‐HFP/PI separators showed suitable air permeability (313.49 s) and higher porosity (71.57%) compared to commercial PP separators. Subsequent heat treatment improved tensile strength from 3.48 to 19.09 MPa. The use of polyimide as the sandwich in PI/PVDF‐HFP/PI sandwich separator causes improved flame retardant properties and minimizes thermal shrinkage (2%). On the other hand, the use of PVDF‐HFP as an intermediate layer with a melting temperature of 148–155°C makes for a safe condition to shut down ta lithium‐ion battery and prevents an explosion. Finally, a coin cell assembled with a pressed and heat‐treated PI/PVDF‐HFP/PI separator showed a discharge capacity of 176 mAh/g at the rate of 0.1C and had higher capacity at the rates above 2C in comparison to the commercial separator (PP).
Quantitative proteomic analyses reveal the impact of nitrogen starvation on the proteome of the model diatom Phaeodactylum tricornutum
Authors: Lupette Josselin , Tardif Marianne , Brugière Sabine , Couté Yohann , Salvaing Juliette , Maréchal Eric 
Published: September 2022

AbstractDiatoms are one of the largest groups in phytoplankton biodiversity. Understanding their response to nitrogen variations, present from micromolar to near‐zero levels in oceans and fresh waters, is essential to comprehend their ecological success. Nitrogen starvation is used in biotechnological processes, to trigger the remodeling of carbon metabolism in the direction of fatty acids and triacylglycerol synthesis. We evaluated whole proteome changes in Phaeodactylum tricornutum after 7 days of cultivation with 5.5‐mM nitrate (+N) or without any nitrogen source (−N). On a total of 3768 proteins detected in biological replicates, our analysis pointed to 384 differentially abundant proteins (DAP). Analysis of proteins of lower abundance in −N revealed an arrest of amino acid and protein syntheses, a remodeling of nitrogen metabolism, and a decrease of the proteasome abundance suggesting a decline in unselective whole‐proteome decay. Analysis of proteins of higher abundance revealed the setting up of a general nitrogen scavenging system dependent on deaminases. The increase of a plastid palmitoyl‐ACP desaturase appeared as a hallmark of carbon metabolism rewiring in the direction of fatty acid and triacylglycerol synthesis. This dataset is also valuable to select gene candidates for improved biotechnological properties.
A genetically encoded far‐red fluorescent calcium ion biosensor derived from a biliverdin‐binding protein
Authors: Hashizume Rina , Fujii Hajime , Mehta Sohum , Ota Keisuke , Qian Yong , Zhu Wenchao , Drobizhev Mikhail , Nasu Yusuke , Zhang Jin , Bito Haruhiko , Campbell Robert E. 
Published: September 2022

AbstractFar‐red and near‐infrared (NIR) genetically encoded calcium ion (Ca2+) indicators (GECIs) are powerful tools for in vivo and multiplexed imaging of neural activity and cell signaling. Inspired by a previous report to engineer a far‐red fluorescent protein (FP) from a biliverdin (BV)‐binding NIR FP, we have developed a far‐red fluorescent GECI, designated iBB‐GECO1, from a previously reported NIR GECI. iBB‐GECO1 exhibits a relatively high molecular brightness, an inverse response to Ca2+ with ΔF/Fmin = −13, and a near‐optimal dissociation constant (Kd) for Ca2+ of 105 nM. We demonstrate the utility of iBB‐GECO1 for four‐color multiplexed imaging in MIN6 cells and five‐color imaging in HEK293T cells. Like other BV‐binding GECIs, iBB‐GECO1 did not give robust signals during in vivo imaging of neural activity in mice, but did provide promising results that will guide future engineering efforts.SignificanceGenetically encoded calcium ion (Ca2+) indicators (GECIs) compatible with common far‐red laser lines (~630–640 nm) on commercial microscopes are of critical importance for their widespread application to deep‐tissue multiplexed imaging of neural activity. In this study, we engineered a far‐red excitable fluorescent GECI, designated iBB‐GECO1, that exhibits a range of preferable specifications such as high brightness, large fluorescence response to Ca2+, and compatibility with multiplexed imaging in mammalian cells.