bluuebunny/crossref_metadata_2025_split huggingface dataset:
Weaving place‐based knowledge for culturally significant species in the age of genomics: Looking to the past to navigate the future
Authors: Rayne Aisling , Blair Stephanie , Dale Matthew , Flack Brendan , Hollows John , Moraga Roger , Parata Riki N. , Rupene Makarini , Tamati‐Elliffe Paulette , Wehi Priscilla M. , Wylie Matthew J. , Steeves Tammy E. 
Published: March 2022

AbstractRelationships with place provide critical context for characterizing biocultural diversity. Yet, genetic and genomic studies are rarely informed by Indigenous or local knowledge, processes, and practices, including the movement of culturally significant species. Here, we show how place‐based knowledge can better reveal the biocultural complexities of genetic or genomic data derived from culturally significant species. As a case study, we focus on culturally significant southern freshwater kōura (crayfish) in Aotearoa me Te Waipounamu (New Zealand, herein Aotearoa NZ). Our results, based on genotyping‐by‐sequencing markers, reveal strong population genetic structure along with signatures of population admixture in 19 genetically depauperate populations across the east coast of Te Waipounamu. Environment association and differentiation analyses for local adaptation also indicate a role for hydroclimatic variables—including temperature, precipitation, and water flow regimes—in shaping local adaptation in kōura. Through trusted partnerships between community and researchers, weaving genomic markers with place‐based knowledge has both provided invaluable context for the interpretation of data and created opportunities to reconnect people and place. We envisage such trusted partnerships guiding future genomic research for culturally significant species in Aotearoa NZ and beyond.
Risk factors for increased perioperative blood loss during total knee arthroplasty in patients with haemophilia
Authors: Kim Myung‐Seo , Kim Jun‐Ho , Kim Kang‐Il 
Published: March 2022

AbstractIntroductionCurrently, there is a paucity of literature describing the risk factors for increased perioperative blood loss (PBL) during total knee arthroplasty (TKA) in haemophilia. Furthermore, no studies have analysed coagulation factor levels that affect PBL.AimTo identify the risk factors for increased PBL by incorporating coagulation factor levels following TKA in patients with haemophilic arthropathy.MethodsA total of 92 TKA (78 haemophilia A and 14 haemophilia B) were performed for haemophilic arthropathy. PBL was calculated using the haemoglobin (Hb) balance method, and patients were categorised into two groups: group H (higher blood loss than the mean PBL, n = 36) and group L (lower blood loss than the mean PBL, n = 56). Body mass index (BMI), operation day Hb, haematocrit and coagulation factor level (VIII or IX) were analysed, including demographic and laboratory data.ResultsThe mean PBL volume during TKA for haemophilic arthropathy of the knee was 542.3 ± 361.7 mL. Multivariate analysis revealed that lower haematocrit on the operation day (odds ratio [OR], .633; 95% confidence interval [CI], .511–.786; p < .001) and coagulation factor level (OR .970, CI .941–.999; p = .046) were independent risk factors for increased PBL. Receiver‐operating characteristic analysis identified these cutoff values for predicting increased PBL: operation day coagulation factor level 93.5% (sensitivity, 70.4%; specificity, 67.6%) and haematocrit level of 38.2% (sensitivity, 85.7%; specificity, 80.6%).ConclusionsThe PBL increased as haematocrit and coagulation factor levels decreased on the operation day. A coagulation factor level <93.5% or haematocrit level of <38.2% may be a significant risk factor for increasing PBL.Level of evidenceLevel IV, Case Series, Treatment Study
A Harungana madagascariensis extract with retinol‐like properties: Gene upregulations and protein expressions in human fibroblasts and skin explants
Authors: Fitoussi Richard , Branchet Marie‐Christine , Garnier Natacha , Beauchef Gallic , Nkengne Alex , Vié Katell , Boisnic Sylvie 
Published: March 2022

AbstractObjectiveBecause they limit, even reverse, age‐induced skin alterations, retinoids became a staple in cosmetology. However, their use can result in undesired secondary effects and there is a demand for natural sources of compounds with retinoid‐like effects. A preliminary screening identified a Harungana madagascariensis plant extract (HME) as possibly inducing genes stimulated by retinol. We analysed its effect on gene and protein expression, comparing it to retinoids.MethodsGene expression was analysed by real‐time qPCR on RNA from isolated fibroblasts subjected to retinol or the plant extract for 6, 48 or 96 h. Skin markers were quantified in fibroblasts cultured with retinol or extract containing medium, and UV‐aged skin explants subjected to topical applications of creams containing retinol, retinaldehyde or HME.ResultsReal‐time qPCR shows that the extract induced all RARs and RXRs, even RXRγ that was not induced by retinol. Eighty‐eight per cent of the 25 early retinoid reaction genes induced by a concentration of retinol are induced by the extract. In fibroblasts, only the extract increased collagen III labelling, while collagen I and fibronectin labelling are increased by retinol and the extract, with higher levels for the extract. When topically applied to UV‐aged skin explants, only the cream containing the HME led to increased labelling of CRABP1 in the epidermis. CRABP2 and Ki67 are induced by all three creams and no effect was detected on RXRs. In the dermisthe extract containing cream increased CRABP2, total collagen, procollagen I and collagen I while creams with retinol or retinaldehyde only affected some of these proteins.ConclusionsThe HME induces an overall retinol‐like gene induction profile in isolated fibroblasts and retinoid‐like stimulation of protein synthesis in both isolated fibroblasts and photoaged skin explants.
Effects of multiple freeze–thaw cycles on meat quality, nutrients, water distribution and microstructure in bovine rumen smooth muscle
Authors: Cao Yinjuan , He Shunzhong , Yu Qunli , Han Ling , Zhang Wei , Zou Xiaohong 
Published: March 2022

SummaryThis study investigated the effect of 5 freeze–thaw cycles (freezing at −18°C for 12 h and then thawing at 4°C for approximately 12 h) on the meat quality, proximate composition, water distribution and microstructure of bovine rumen smooth muscle (BSM). As the number of freeze–thaw cycles increased, BSM pH, shear force, water content and protein content decreased by 3.06%, 35.50%, 14.49% and 21.11%, respectively, whereas BSM thawing loss, cooking loss, pressing loss, total aerobic count (TAC), ash content and fat content increased by 108.12%, 47.75%, 78.33%, 90.99%, 105% and 35.20%, respectively. The freeze–thaw cycles resulted in greater protein and lipid oxidation, as evidenced by a 36.46% reduction in the sulfhydryl content and a 209.06% and 338.46% increase in the carbonyl and malondialdehyde contents, respectively. Ice crystal formation disrupted the structural integrity of the muscle tissue. Low‐field nuclear magnetic resonance results showed that the freeze–thaw cycles prolonged the relaxation times (T2b, T21 and T22), indicating that immobile water shifted to free water, and consequently, free water mobility increased. After 3 freeze–thaw cycles, the decline in shear force slowed, the increase in thawing loss became accelerated, and the TAC approached the domain value (6 log colony‐forming units/g). Therefore, the number of freeze–thaw cycles of smooth muscle during transport, storage and distribution should be controlled to 3 or fewer. The current results provide a theoretical basis and data support for the further utilisation and culinary processing of smooth muscle.
Quality assessment of ready‐to‐eat Pacific saury (Cololabis saira) using microwave‐assisted thermal sterilisation combined with olive oil vacuum impregnation
Authors: Jiang Jiale , Zhang Mei , Luan Donglei , Xu Changhua , Hua Chuanxiang , Zhu Qingcheng , Tao Ningping 
Published: March 2022

SummaryAlthough microwave‐assisted thermal sterilisation (MATS) held great potential to sterilise ready‐to‐eat (RTE) products compared with traditional retort sterilisation, the quality improvement effect of MATS treatment on shelf‐stable RTE products has not been extensively studied. This study was performed to evaluate the efficacy and applicability of MATS combined with olive oil vacuum impregnation against lipid oxidation and microbial growth in RTE Pacific saury during storage. MATS combined with olive oil vacuum impregnation (MTSO) effectively reduced processing time (by 57%) compared to retort‐treated RTE Pacific saury at the same thermal lethality value. The lower cooking loss (6.19%) and colour damage indicated that MTSO enhanced water retention and the stability of tissue structure compared to retort‐treated group (9.88%) or no olive oil group (7.11%). Lipid oxidation and protein degradation were effectively inhibited in MATS groups compared to retort‐treated ones during storage; such effect was more effective in olive oil impregnated group. Likewise, microbial activity and electronic sensory evaluations showed that the shelf life of MTSO group was extended by 1 week compared to retort‐treated groups. Overall, MTSO had considerable industrial potential to yield RET Pacific saury and even RTE products with better quality attributes than retort‐treated ones.
Phenolic compounds and antioxidant activity are differentially affected by drying processes in celery, coriander and parsley leaves
Authors: Mouhoubi Khokha , Boulekbache‐Makhlouf Lila , Madani Khodir , Palatzidi Anastasia , Perez‐Jimenez Jara , Mateos‐Aparicio Inmaculada , Garcia‐Alonso Alejandra 
Published: March 2022

SummaryConvective drying (CO) is the most common technique for drying herbs, although it may diminish phenolic compounds content. Microwave drying (MW) has been suggested as an alternative, although its effects on phenolic compounds remain to be explored. The aim of this study is to compare the effects of different convective temperatures (40, 80 and 120 °C) and microwave powers (100, 500 and 1000 W) on drying time, polyphenols classes (HPLC‐DAD) and antioxidant capacity in celery, coriander and parsley leaves. Microwave procedure reduced drying time (MW: 3 h–7 min; CO: 25–1.5 h) resulting more effective than CO. Increasing temperatures led to an initial decrease in total phenolic content (TPC) and antioxidant activity followed by an increase, potentially by the release of bound phenolic acids. Regarding MW, the highest polyphenol content was generally observed at 500 W, probably due to the hydroxycinnamic acid's rise. Antioxidant capacity was similar at 500 and 1000 W. There was a good agreement between TPC and antioxidant capacity. Consequently, MW seems a good alternative to reduce drying time. However, the maintenance or improvement of phenolic activity depends on the food matrix. Indeed, the best drying system and conditions to preserve phenolic compounds were variable depending on the herb type: celery (CO at 40 °C), coriander (both CO at 40 °C and MW at 500 W) and parsley (MW at 100 W).
Characterization of the glutathione S‐transferase genes in the sand flies Phlebotomus papatasi and Lutzomyia longipalpis shows expansion of the novel glutathione S‐transferase xi (X) class
Authors: Ashraf Faisal , Weedall Gareth D. 
Published: March 2022

AbstractLeishmaniasis control often relies upon insecticidal control of phlebotomine sandfly vector populations. Such methods are vulnerable to the evolution of insecticide resistance via a range of molecular mechanisms. There is evidence that two major resistance mechanisms, target site insensitivity and metabolic resistance, have evolved in some sandfly populations and further genetic characterization of resistance would be useful to understand and combat it. To facilitate the study of the mechanisms of metabolic resistance, here we improved the annotation and characterized a major detoxification gene family, the glutathione‐s‐transferases (GST), in the genomes of two sand fly species: Phlebotomus papatasi and Lutzomyia longipalpis. The compositions of the GST gene family differ markedly from those of Aedes and Anopheles mosquitoes. Most strikingly, the xi (X) class of GSTs appears to have expanded in both sand fly genomes. Our results provide a basis for further studies of metabolic resistance mechanisms in these important disease vector species.
The burden of being certain: National identity certainty predicts support for COVID‐Related restrictive measures and outgroup conspiracy beliefs
Authors: Chen Kaiyuan , Zhang Jinghui , Ao Xiang , Ramdass Jeff 
Published: March 2022

AbstractIn dealing with the COVID‐19 pandemic, government officials often encounter two concurrent concerns: they have to enforce necessary public health and safety measures to manage COVID‐19. Meanwhile, they also have to mitigate conspiracy beliefs about COVID‐19. To shed light on these issues, we conducted two studies to investigate national identity certainty (i.e., the extent to which people are certain about their national identity) as a predictor of (a) support for restrictive measures to curtail COVID‐19 and (b) conspiracy beliefs about an outgroup as the culprit of COVID‐19. Study 1 was a three‐week longitudinal study (N = 301) where we investigated the relationships both on a between‐person level (differences between individuals) and on a within‐person level (week‐by‐week fluctuations of the same individual). We found that individual differences in national identity certainty predicted increased support for restrictive measures and increased outgroup conspiracy beliefs. These relationships emerged, even when we controlled for national identity positivity, that is, the extent to which people see their national identity in positive light. In Study 2 (N = 316), we used a cross‐sectional correlational design and replicated the findings of Study 1. Moreover, we found that the relationships were explained by distinct threat perceptions: realistic threat explained the increased support for restrictive measures, whereas symbolic threat explained the increased outgroup conspiracy beliefs. Overall, our findings suggest that support for restrictive measures and outgroup conspiracy beliefs can be seen as attempts of people high in national identity certainty to address the distinct threats of COVID‐19.
Antimicrobial effect of chitosan and extracellular metabolites of Pediococcus pentosaceus CM175 against Salmonella Typhimurium and Escherichia coli O157:H7
Authors: Enciso‐Martínez Yessica , González‐Pérez Cristóbal J. , Aispuro‐Hernández Emmanuel , Vargas‐Arispuro Irasema C. , Ayala‐Zavala Jesús F. , Martínez‐Téllez Miguel A. 
Published: March 2022

AbstractFoodborne illness outbreaks caused by consuming cantaloupe melon contaminated with Salmonella spp. and Escherichia coli O157:H7 are recurrent. The objective of this work was to investigate the antimicrobial effect of the combination of chitosan (Q) and extracellular metabolites of Pediococcus pentosaceus CM175 (EmPp) on the surface of cantaloupe against E. coli O157:H7 and Salmonella Typhimurium. The minimum inhibitory and bactericide concentrations (MIC and MBC) of Q and EmPp were determined by the method of microdilution in broth and viability in plate, respectively. The antimicrobial effect derived from the combination of Q and EmPp was evaluated using the chessboard technique. The antimicrobial activity of the different treatments in cantaloupe was performed employing plate counting. The viability and membrane integrity of S. Typhimurium and E. coli O157:H7 after treatments application were observed by fluorescence microscopy. The results showed that MIC and MBC of Q against S. Typhimurium were 0.1% and 0.7%, respectively; 0.004% and 0.03% for E. coli O157:H7, respectively; for EmPp, MIC was 8.0% and MBC was 10% for both bacteria. The combination of 0.1% Q and 6.6% EmPp against S. Typhimurium and 0.01% Q and 5.0% EmPp against E. coli O157:H7 showed an additive effect. The highest reductions of S. Typhimurium and E. coli O157:H7 in cantaloupe were exerted by the combinations of 0.1% Q with 6.6% EmPp and 0.01% Q with 5.0% EmPp, respectively. These results show that combination of Q and EmPp could be an effective and promising alternative against the tested pathogens.
Sphingolipid synthesis inhibitor fumonisin B1 causes verticillium wilt in cotton
Authors: Xu Fan , Huang Li , Wang Junyao , Ma Caixia , Tan Yingqing , Wang Fanlong , Fan Yanhua , Luo Ming 
Published: March 2022

SUMMARYVerticillium wilt caused by Verticillium dahliae is a major disease of cotton. Acidic protein–lipopolysaccharide complexes are thought to be the toxins responsible for its symptoms. Here, we determined that the sphingolipid biosynthesis inhibitor fumonisin B1 (FB1) acts as a toxin and phenocopies the symptoms induced by V. dahliae. Knocking out genes required for FB1 biosynthesis reduced V. dahliae pathogenicity. Moreover, we showed that overexpression of a FB1 and V. dahliae both downregulated gene, GhIQD10, enhanced verticillium wilt resistance by promoting the expression of brassinosteroid and anti‐pathogen genes. Our results provide a new strategy for preventing verticillium wilt in cotton.
Decisional conflicts, anxiety, and perceptions of shared decision‐making in cancer treatment trajectory among adolescents with cancer: A longitudinal study
Authors: Wu Li‐Min , Chiou Shyh‐Shin , Lin Pei‐Chin , Liao Yu Mei , Su Hsiu‐Lan 
Published: March 2022

AbstractPurpose: To examine the trajectory of decisional conflict and anxiety experienced by adolescents after the cancer diagnosis, and explore their perceptions on participation in shared decision‐making (SDM). Design: This longitudinal study used incorporated data from questionnaires and interviews. Methods: Participants recruited from an academic hospital in southern Taiwan ranged in age from 13 to 20 years with a cancer diagnosis within 1 month and received cancer treatment. Each participant completed questionnaires on decisional conflict and anxiety at diagnosis, 1, 3, and 6 months later. Individual interviews were to gain an in‐depth understanding of SDM. Findings: Total scores on decisional conflict changed significantly over time (F = 2.98, p = 0.039); the scores at 1 month were higher than 3 months (t = 2.18, p = 0.04) and 6 months (t = 2.97, p = 0.008). Participants perceived significantly different levels of values clarify (F = 9.49, p < 0.01) and support (F = 8.46, p < 0.01) over time. Only 27.3% of participants were anxiety‐free. No significant differences were found in anxiety over time. The perception of SDM was a situational involvement. Conclusions: Decisional conflict changed over time. Participants experienced greater decisional conflict at 4–8 weeks after diagnosis and their anxiety did not decrease over time. The different levels of participation in SDM during their treatment trajectory were found. Clinical Relevance: Participants experienced the highest decisional conflict during diagnosis, and highlighted how their roles in healthcare discussions varied from direct participation to indirect involvement. Further research is needed to develop an SDM model which accommodates different levels of needs and implements timely support.
Botanical extracts in combination improve autosomal recessive woolly hair/hypotrichosis caused by LIPH mutations
Authors: Qu Baoquan , Meng Shengnan , Yang Chaowei , Lv Shuying , Lin Wenjun , Yang Dingquan 
Published: March 2022

ABSTRACTBackgroundMutation in the lipase H (LIPH) gene is a main reason for autosomal recessive woolly hair (ARWH)/hypotrichosis. Although some studies reported that topical minoxidil could improve ARWH, an effective treatment method for this disease is still lacking.AimWe attempt to explore potential treatment options for ARWH.Materials & MethodsA female 6‐year‐old child was diagnosed with ARWH/hypotrichosis caused by LIPH mutations. And she was treated with combined treatment of botanical extracts.ResultsAfter 6 months of treatment, the patient's hair grew remarkably. After 4 years of treatment, the patient's hair remained dense.DiscussionAfter the combination treatment, the patient saw a favorable clinical effect. However, the specific mechanisms of action for botanical extracts require further validation. In addition, some regenerative strategies may be considered as potential treatment options for ARWH. We should actively attempt treatment for ARWH patients and encourage prenatal diagnosis due to the great impact of hair loss.ConclusionThe combined therapy of botanical extracts may improve ARWH long‐term with a sustainable therapeutic effect.
Development of a cream formulation containing bee venom and other bee products
Authors: Tanuğur Samancı Aslı Elif , Kekeçoğlu Meral 
Published: March 2022

AbstractThis study aimed to develop a prototype skincare product with bee venom, propolis, honey, beeswax, and royal jelly. The prototype formulation contained 0.1% bee venom, 0.3% propolis extract, 0.45% honey, and 1.0% royal jelly. The prototype body cream was analyzed for stability, antioxidant activity, dermatological response, and cytotoxicity. In addition, a panel test evaluated the prototype for the claims such as skin smoothness, feelings of nourishment, moisturizing, skin tone, brightness, and visibility of wrinkles. According to the stability test, the prototype was stable for up to 90 days at room temperature and +40°C. The formulation was found to have a high antioxidant capacity at 85.45%. Cell viability detected over 70% indicated that the prototype body cream was not cytotoxic. The dermatological analysis revealed no irritation or allergic reaction in non‐allergic individuals. Panel test showed that the prototype makes skin silky smooth, contributes to hydration, brightens and nourishes the skin, evens the skin tone, reduces the visibility of wrinkles, improves skin elasticity, and smoothes wrinkles. This prototype formulation requires further research to evaluate its effectiveness against skin aging on different skin types. Nevertheless, the side effects of such products need particular attention in developing a commercial product containing bee venom in susceptible individuals.
Topical finasteride for male and female pattern hair loss: Is it a safe and effective alternative?
Authors: Gupta Aditya K. , Talukder Mesbah 
Published: March 2022

AbstractBackgroundOral finasteride is an FDA‐approved treatment for androgenetic alopecia (AGA). Topical finasteride, while not FDA‐approved, lacks the systemic adverse effects associated with oral finasteride. The efficacy of topical finasteride has been evaluated.AimTo review whether topical finasteride is a safe and effective treatment for male and female pattern hair loss.MethodA structured search in PubMed and Google Scholar identified 864 records, with 32 articles meeting the inclusion criteria for review.Results and discussionIn a phase III, randomized, controlled trial, the efficacies of topical 0.25% w/w finasteride spray (1–4 sprays; 50–200 μl/day) and once‐daily finasteride 1 mg oral tablet were similar when administered for 24 weeks (mean change from baseline, 20.2 vs. 21.1 hairs/cm2). Additionally, a double‐blind, randomized trial compared the efficacies of twice‐daily finasteride 1% topical gel and once‐daily finasteride 1 mg oral tablet for 6 months, and found similar results in both groups. Moreover, a combination of topical minoxidil and topical finasteride may enhance efficacy.Topical finasteride reduces both scalp and plasma DHT levels. In an open‐label pharmacodynamic study, a 7‐day treatment of twice‐daily finasteride 0.25% topical solution and once‐daily finasteride 1 mg oral tablet provided similar inhibition of plasma DHT.Topical finasteride reduces the potential for systemic side effects, including the risk of sexual dysfunction. The side effects are localized to the application site, for example, scalp pruritus, burning sensation, irritation, contact dermatitis, and erythema.ConclusionTopical finasteride may be an alternative for those concerned about the oral formulation's systemic side effects.