bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of miR‐3182 and miR‐3143 target genes involved in the cell cycle as a novel approach in TNBC treatment: A systems biology approach
Authors: Khazaei‐Poul Yalda , Mirmotalebisohi Seyed Amir , Zali Hakimeh , Molavi Zahra , Mohammadi‐Yeganeh Samira 
Published: October 2022

AbstractTriple‐negative breast cancer (TNBC) is an aggressive subtype of breast cancer with a poor prognosis, lacking therapeutic targets. miRNAs play crucial roles in TNBC through regulating various mechanisms, including cellular growth and proliferation. This study aims to identify critical target genes of two novel miRNAs (miR‐3143 and miR‐3182) involved in the cell cycle of TNBC as possible therapeutic targets and investigates their regulatory and therapeutic roles through a systems biology approach and in vitro experiment. Datasets related to the TNBC cell line (MDA‐MB‐231) were screened and retrieved, and Gene regulatory networks were constructed. Significant regulatory motifs were detected and analyzed using the FANMOD and Cytoscape analyzer, and the clusters and seeds were identified using the MCODE. Functional enrichment analysis was also performed using DAVID and STRING. The most critical genes were determined using the analysis of GRN motifs and PPI clusters. The essential genes involved in the cell cycle were selected and verified using the bc‐GenExMiner v4.7. We overexpressed miR‐3143 and miR‐3182 in the MDA‐MB‐231 cell line using human umbilical cord mesenchymal stem cell (HUCMSC)‐miRNA loaded exosomes, and the expression of the critical target genes was investigated using RT‐qPCR. We identified eight critical genes as potential therapeutic targets. Their expression decreased by overexpression of miR‐3143 and miR‐3182 in RT‐qPCR. The identified critical genes have probably significant roles in the pathogenesis of TNBC through the cell cycle. We suggest that the overexpression of miR‐3143 and miR‐3182 could be a new therapeutic candidate in TNBC and is worth more investigation.
Enablers and barriers to diabetic retinopathy eye care among first nations and Métis women
Authors: Umaefulam Valerie , Premkumar Kalyani 
Published: October 2022

AbstractBackgroundDiabetes is increasingly prevalent in Indigenous women and increases their risk of developing diabetic retinopathy, an eye complication of diabetes and a common cause of vision loss in Canada, especially among adults. Early detection is the most effective approach to prevent vision loss and reduce the impact of diabetic retinopathy.ObjectiveThis study examined enablers and barriers that influence the diabetes eye care behaviour of First Nations and Métis women with diabetes and at risk of diabetes.MethodsWe conducted a descriptive qualitative study with 35 First Nations and Métis women with diabetes or at risk of diabetes in Saskatoon, Canada. Data were collected via four sharing circle discussions and were analysed using thematic analysis.ResultsThe study findings showed that understanding of diabetes eye care access and cost, and unsupportive interactions with health care practitioners, were barriers to diabetic retinopathy care behaviour. Conversely, the presence of eye complications, participants' resolve to manage diabetes, self‐efficacy and fear due to experiences of family members with diabetes enabled diabetes eye care.ConclusionsOur study advances knowledge in socio‐cultural factors influencing diabetic retinopathy care behaviour among First Nations and Métis women living with and at risk of diabetes. The study shows the need for further public health and health system interventions to address barriers and support Indigenous peoples with or at risk of diabetes to make informed health decisions.
Relationship between early neural responses to syntactic and acoustic irregularities in music
Authors: Ishida Kai , Nittono Hiroshi 
Published: October 2022

AbstractHumans can detect various anomalies in a sound sequence without attending to each dimension explicitly. Event‐related potentials (ERPs) have been used to examine the processes of auditory deviance detection. Previous research has shown that music‐syntactic anomalies elicit early right anterior negativity (ERAN), whereas more general acoustic irregularities elicit mismatch negativity (MMN). Although these ERP components occur in a similar latency range with a similar scalp topography, the relationship between the detection processes they reflect remains unclear. This study compared these components by manipulating music‐syntactic (chord progression) and acoustic (intensity) irregularities orthogonally in two experiments. Non‐musicians (Experiment 1: N = 39; Experiment 2: N = 24) were asked to listen to chord sequences, each consisting of 5 four‐voice chords, as they watched a silent video clip. Standard, harmonic‐deviant, intensity‐deviant and double‐deviant chords occurred at the final position in each sequence. Deviant stimuli were presented infrequently (p = .10) in Experiment 1 and equiprobably (p = .25) in Experiment 2. Regardless of deviance probability, both harmonic and intensity deviants elicited similar negativities, which were indistinguishable in terms of latency or scalp distribution. When the two deviant types occurred simultaneously, the negativity increased in an additive manner; that is, the amplitude of the double‐deviant ERP was as large as the sum of the single‐deviant ERPs. These findings suggest that the detection of music‐syntactic and acoustic irregularities works independently, based on different regularity representations.
New ‘kids’ on the voltage‐gated proton channel block
Authors: Korpos Éva , Papp Ferenc 
Published: October 2022

So far one gene for Hv1 has been detected in studied species. The work presented by Chaves et al. in The FEBS Journal reported an ‘Unexpected expansion of the voltage‐gated proton channel family’. They searched for proton channel candidates and found three sequences in the genome of Aplysia californica (Ac), which were named AcHv1, AcHv2 and AcHv3. Based on electrophysiological experiments, AcHv1 and AcHv2 are voltage‐gated channels. While AcHv1 behaves like Hv1 in other species, that is, it is voltage and pH‐dependent, it can be inhibited by zinc and conducts protons outwardly, AcHv2 conducts protons inwards at symmetrical pH. AcHv3 constantly leaks protons, and its C‐terminal part contains several cytoplasmic retention motifs. Through carefully designed and carried out electrophysiological experiments, Chaves et al. determined the biophysical parameters of all three proton channels, such as the voltage and the pH dependence, the threshold‐voltage, the gating charge and the time constants of activation and inactivation.Comment on: https://doi.org/10.1111/febs.16617.
Modelling the longitudinal distribution, abundance, and habitat use of the giant freshwater shrimp (Macrobrachium spinipes) in a large intermittent, tropical Australian river to inform water resource policy
Authors: Beesley Leah S. , Killerby‐Smith Savannah , Gwinn Daniel C. , Pusey Bradley J. , Douglas Michael M. , Novak Peter A. , Tayer Thiaggo C. , Keogh Chris S. , Kennard Mark J. , Canham Caroline A. , Setterfield Samantha A. 
Published: October 2022

Abstract

Water development threatens rivers and their biodiversity. Amphidromous shrimp are particularly vulnerable as they require migration between freshwater and estuaries to complete their life cycle. The Fitzroy River is a large tropical intermittent river undergoing water development that is home to the amphidromous shrimp Macrobrachium spinipes (cherabin), yet little is known about its habitat use and flow‐ecology making it difficult to inform sustainable water‐take.

We investigated habitat associations, distributional patterns suggestive of amphidromy, and the influence of water availability by sampling main channel and floodplain pools along a 350‐km river length during 2 contrasting flow years. Applying a size‐specific abundance model, we estimated abundance per size class, site, and year. We then predicted abundance at the landscape scale with remotely sensed water to reveal the impact of water availability on the meta‐population.

Our model revealed that juveniles were in greatest abundance in downstream main channel pools, whereas adults were in greatest abundance in upstream floodplain pools. Abundance varied by year with lower numbers predicted in the low‐flow year. Longitudinal and habitat patterns remained when our pool‐level results were scaled to the landscape, and the positive relationship of abundance to wet‐season flow was strengthened. The predominance of smaller cherabin in the lower reaches of the river provides indirect support for an estuarine nursery and amphidromous life history; however, small individuals observed in landlocked pools, during late dry season suggests possible within‐river recruitment.

The importance of water development policies that protect wet‐season flow and passage along the Fitzroy River is supported by this work. These types of policies are likely to be important for this and other amphidromous shrimp species across Australia, Southeast Asia and further afield. Further research detailing the species life history and describing flow–recruitment relationships will be important contributions to understanding this important taxonomic group and refining policies for current and future water resource development.
Perspectives of choice and control in daily life for people following brain injury: A qualitative systematic review and meta‐synthesis
Authors: Murray Carolyn M. , Weeks Scott , van Kessel Gisela , Guerin Michelle , Watkins Emma , Mackintosh Shylie , Fryer Caroline , Hillier Susan , Stanley Mandy 
Published: October 2022

AbstractBackground and ObjectiveAcquired brain injury (ABI) can result in considerable life changes. Having choice and control over daily life is valued by people following ABI. This meta‐synthesis will analyse and integrate international research exploring perspectives of choice and control in daily life following ABI.MethodsDatabases were searched from 1980 to 13 January 2022 for eligible qualitative studies. After duplicates were removed, 22,768 studies were screened by title and abstract, and 241 studies received full‐text assessment with 56 studies included after pearling. Study characteristics and findings were extracted that related to personal perspectives on choice and control by people with an ABI (including author interpretation and quotes). Data from each study were coded and then segments of coded data across the studies were compared to create multiple broad categories.FindingsFindings were then reduced from categories into 3 overarching themes with 12 subthemes. These themes were: (1) feeling like a second‐class citizen; (2) reordering life and (3) choosing a path. Participants with an ABI tussled between their feelings of loss following brain injury and their thinking about how they start to regain control and become agents of their own choices. The themes describe their sense of self, their changed self and their empowered self in relation to ‘choice and control’.ConclusionsRe‐engaging with choice and control after ABI is dynamic and can be challenging. Health professionals and supporters need to facilitate a gradual and negotiated return to agency for people following ABI. A sensitive and person‐centred approach is needed that considers the readiness of the person with ABI to reclaim choice and control at each stage of their recovery. Clear service or process indicators that are built on lived experience research are needed to facilitate changes in service delivery that are collaborative and inclusive.Patient or Public ContributionThis review included the voices of 765 people living with ABI and was conducted by a diverse team of allied health professionals with practice knowledge and research experience with people following ABI. Twenty‐nine of the 56 included studies had participants contributing to their design or analysis.
How shared is decision‐making in multidisciplinary tumour conferences with patient participation? An observational study
Authors: Schellenberger Barbara , Heuser Christian , Diekmann Annika , Ernstmann Nicole , Schippers Anna , Geiser Franziska , Schmidt‐Wolf Ingo G. H. , Scholl Isabelle , Ansmann Lena 
Published: October 2022

AbstractBackgroundIn some breast and gynaecologic cancer centres in Germany, patients participate in their own case discussion in multidisciplinary tumour conferences (MTCs), where treatment recommendations are discussed and finalized. However, the extent to which patients in MTCs are involved in decision‐making on treatment recommendations remains largely unexplored. Hence, this study investigates how recommendations are communicated to patients and the extent to which the interactions with patients in MTCs are in line with shared decision‐making (SDM).MethodsIn this observational study, we audio‐recorded MTCs with patient participation in three breast and gynaecologic cancer centres in Germany. We qualitatively analysed the data with regard to content and linguistic aspects.ResultsWe analysed 82 case discussions. Recommendations made during MTCs were regarding (i) treatment options, (ii) treatment initiation, (iii) next (treatment) steps and (iv) whether a treatment method should be initiated at all. The decision about recommendations depended in part on patients' preferences or further course/further outcomes. Although the purpose of MTCs is to provide recommendations, some recommendations were framed as the final decision. The majority of the decision‐making conversation could be characterized as option talk (78%), during which patients were mostly proposed only one (treatment) option.ConclusionsThis study establishes limited SDM in MTCs with patient participation. By indicating choices and thereby creating awareness of choices among patients, MTCs with patient participation could be used to foster SDM implementation.Patient or Public ContributionTwo representatives of a large self‐help organization for patients with breast cancer assisted the research project, particularly, in discussing the results.
Cerebrospinal fluid neurofilament light and cerebral atrophy in younger‐onset dementia and primary psychiatric disorders
Authors: Walia Nirbaanjot , Eratne Dhamidhu , Loi Samantha M. , Farrand Sarah , Li Qiao‐Xin , Malpas Charles B. , Varghese Shiji , Walterfang Mark , Evans Andrew H. , Parker Shaun , Collins Steven J. , Masters Colin L. , Velakoulis Dennis 
Published: October 2022

AbstractBackground and AimsNeurodegeneration underpins the pathological processes of younger‐onset dementia (YOD) and has been implicated in primary psychiatric disorders (PSYs). Cerebrospinal fluid (CSF) neurofilament light (NfL) has been used to investigate neurodegeneration severity through correlation with structural brain changes in various conditions, but has seldom been evaluated in YOD and PSYs.MethodsThis retrospective study included patients with YOD or PSYs with magnetic resonance imaging (MRI) of the brain and CSF NfL analysis. Findings from brain MRI were analysed using automated volumetry (volBrain) to measure white matter (WM), grey matter (GM) and whole brain (WB) volumes expressed as percentages of total intracranial volume. Correlations between NfL and brain volume measurements were computed whilst adjusting for age.ResultsSeventy patients (47 with YOD and 23 with PSY) were identified. YOD types included Alzheimer disease and behavioural variant frontotemporal dementia. PSY included schizophrenia and major depressive disorder. MRI brain sequences were either fast spoiler gradient‐echo (FSPGR) or magnetization‐prepared rapid acquisition gradient‐echo (MPRAGE). In the total cohort, higher NfL was associated with reduced WB in the FSPGR and MPRAGE sequences (r = −0.402 [95% confidence interval (CI), −0.593 to −0.147],P = 0.008 andr = −0.625 [95% CI, −0.828 to −0.395],P < 0.001, respectively). Higher NfL was related to reduced GM in FSPGR (r = 0.385 [95% CI, −0.649 to −0.014],P = 0.017) and reduced WM in MPRAGE (r = −0.650 [95% CI, −0.777 to −0.307],P < 0.001). Similar relationships were seen in YOD, but not in PSY.ConclusionHigher CSF NfL is related to brain atrophy in YOD, further supporting its use as a nonspecific marker of neurodegeneration severity.
Effect of thermosonication on physicochemical and anti‐nutritional properties of blood fruit beverage
Authors: Sasikumar Raju , Jaiswal Amit K. 
Published: October 2022

AbstractThe study evaluates the effectiveness of thermosonication treatment on the physicochemical and anti‐nutritional properties of blood fruit beverage. The study has revealed that physicochemical properties such as pH, titratable acidity, color difference, total soluble solids, ascorbic acid content, total polyphenols, total anthocyanins, total flavonoids, and DPPH retained as the fresh juice. Similarly, anti‐nutritional components such as phytate, saponin, alkaloids, tannin, and oxalates were lowered significantly by the TST (Thermosonication treatment) TST‐1 (40°C/30 min), TST‐2 (42°C/27 min), TST‐3 (44°C/24 min), TST‐4 (46°C/21 min), TST‐5 (48°C/18 min), TST‐6 (50°C/15 min), and TST‐7 (52°C/12 min). Thermosonicated beverages had an extended shelf life of 4 weeks at 4°C with desirable physicochemical quality, and the storage study has exhibited minimal quality changes. The results obtained from this study may help the fruit beverage industries design similar processing of blood fruit beverages on a large‐scale production.Practical applicationsCommercialization of blood fruit juice by value addition and lowering of its anti‐nutritional components may result in its availability all over the world along with its nutritious intake and healthy living among people. Thermosonically treated blood fruit juice with the unique parameter combination have resulted in better retention of phytonutrients as well as reduction in its anti‐nutritional factors, thereby offering excellent product quality as compared to the conventionally pasteurized beverages. This might increase the potential for commercialization of blood fruit and also result in the development of a trusted area for startup of this beverage industries, such as iron‐rich fruit beverage (58.16 mg/100) which will again promote better nutritional status, malnutrition free, and better livelihood in the local areas with ample blood fruit availability.
Delayed circadian rhythms in older Africans living with human immunodeficiency virus (HIV)
Authors: Redman Kirsten N. , O'Brien Katie E. , Ruiz Francieli S. , Rae Dale E. , Gómez‐Olivé F. Xavier , von Schantz Malcolm , Scheuermaier Karine 
Published: October 2022

AbstractThe increasing number of people living with human immunodeficiency virus, HIV, (PLWH) have an elevated incidence of risk for noncommunicable comorbidities, the aetiology of which remains incompletely understood. While sleep disturbances are often reported in PLWH, it is unknown to what extent they relate to changes in the circadian and/or sleep homeostatic processes. We studied the relationship between sleep characteristics, circadian phase, and HIV status in older adults from the HAALSI (Health and Ageing in Africa: a Longitudinal Study of an INDEPTH Community in South Africa) subsample of the Agincourt Health and Demographic Surveillance System in South Africa (n = 187, 36 human immunodeficiency virus positive [HIV+], age: 66.7 ± 11.5 years, range 45—93 years), where HIV prevalence is high and (in contrast to the global north) does not associate significantly with potentially confounding behavioural differences. In participants with valid actigraphy data (n = 172), regression analyses adjusted for age and sex indicated that HIV+ participants had slightly later sleep onset (β = .16, p = .039), earlier sleep offset times (β = −.16, p = .049) and shorter total sleep times (β = −.20, p = .009) compared to the HIV negative (HIV−) participants. In a subset of participants (n = 51, 11 HIV+), we observed a later dim light melatonin onset (DLMO) in HIV+ (21:16 ± 01:47) than in HIV− (20:06 ± 00:58) participants (p = .006). This substantial difference remained when adjusted for age and sex (β = 1.21; p = .006). In 36 participants (6 HIV+) with DLMO and actigraphy data, median phase angle of entrainment was −6 min in the HIV+ group and +1 h 25 min in the HIV− group. DLMO time correlated with sleep offset (ρ = 0.47, p = .005) but not sleep onset (ρ = −0.086, p = .623). Collectively, our data suggest that the sleep phase occurred earlier than what would be biologically optimal among the HIV+ participants. This is the first report of a mistimed circadian phase in PLWH, which has important potential implications for their health and well‐being, especially given the well‐established relationships between circadian asynchrony and sleep deprivation with poorer health outcomes.
Quality and content analysis of female urethroplasty videos on YouTube
Authors: Sahin Yusuf , Paslanmaz Filip , Ulus Ismail , Yilmaz Mehmet , Dincer Muhammet Murat , Muslumanoglu Ahmet Yaser 
Published: October 2022

AbstractObjectivesIn this study, we aimed to analyze scientific quality and content of female urethroplasty videos on YouTube.MethodsWe searched YouTube using the “female urethroplasty”, “female urethral stricture”, and “urethroplasty” keywords on February 22, 2022. The quality and content of videos were analyzed using the Global Quality Score (GQS) and Female Urethroplasty‐Specific Checklist Score (FUSCS) which was developed by our clinic. Video analysis was performed by two independent urologists. The relationship between the video characteristics and GQS and FUSCS was examined.ResultsA total of 38 videos were analyzed. Fourteen (36.8%) videos were uploaded by academic sources such as urology societies and universities/hospitals, while 24 (63.2%) videos were uploaded by urologists. The median GQS was 3 (range, 2–4) and the median FUSCS was 8 (range, 5–9) for all videos. The Cohen's kappa was 0.834 for GQS and 0.899 for FUSCS, indicating a high level of agreement between the observers. The median GQS was 4 (range, 4–5) and the median FUSCS was 9 (range, 8–10) for academic videos, indicating a statistically significantly higher scores than the urologists videos (p = .002 and p < .001, respectively).ConclusionAcademic videos on female urethroplasty on YouTube have adequate scientific quality and content for both patients and healthcare professionals. The number of videos by academic sources on female urethroplasty should be increased and individuals should be encouraged to search such videos on search engines.
Anchor‐free arbitrary‐oriented construction vehicle detection with orientation‐aware Gaussian heatmap
Authors: Guo Yapeng , Xu Yang , Niu Jin , Li Shunlong 
Published: October 2022

AbstractEffective detection of arbitrary‐oriented construction vehicles is critical for ensuring construction site safety. Current construction vehicle detection methods are mostly anchor‐based, which require complex manual setting for anchor proposals. This study proposes an anchor‐free network for arbitrary‐oriented construction vehicle detection with an orientation‐aware Gaussian heatmap, which constructs a more appropriate intermediate state and provides more learnable information to accelerate training convergence and improve inference accuracy. The proposed network comprises feature extraction and regression parts. The former is used to extract multilevel features and restore the spatial information of construction vehicles, while the latter regresses the identification tuple including center point position, offset, width, height, and orientation angle in orientation‐aware bounding boxes. Moreover, this study created a multiscale dataset captured from 18 different actual construction sites for training and verification, including 600 images and 1570 construction vehicles. Comparisons with different state‐of‐the‐art methods (including anchor‐based, anchor‐free and segmentation‐based methods) demonstrate the accuracy and effectiveness of the proposed method.