bluuebunny/crossref_metadata_2025_split huggingface dataset:
TGF-β1 promotes human breast cancer angiogenesis and malignant behavior by regulating endothelial-mesenchymal transition
Authors: Li Zi-Xiong , Chen Jie-Xin , Zheng Ze-Jun , Cai Wang-Jing , Yang Xiong-Bin , Huang Yuan-Yuan , Gong Yao , Xu Feng , Chen Yong-Song , Lin Ling 
Published: November 2022

BackgroundEndothelial-mesenchymal transition (EndMT) is an important process of angiogenesis, which plays a significant role in in tumor invasion and metastasis, while its regulatory mechanisms in breast cancer remain to be fully elucidated. We previously demonstrated that tumor-associated macrophages (TAMs) can induce EndMT in endothelial cells by secreting CCL18 through the activation of the TGF-β and Notch signaling pathways in breast cancer. This study was designed to study the role of EndMT in breast cancer angiogenesis and progression in order to explore the underlying mechanism.MethodsImmunohistochemistry (IHC) was used to evaluate the expression of microvascular density (MVD) and EndMT markers in breast cancer. TGF-β1 was used to induce EndMT models of differentiated-endothelial breast cancer stem-like cells (BCSLCs). In vitro cell migration, proliferation and matrigel tube-formation assays, as well as in vivo nude mouse tumor-bearing model and nude mouse dorsal skinfold window chamber (DSWC) model, were utilized to investigate the effects in order to explore the mechanism of EndMT induced by TGF-β1 on breast cancer progression.ResultsIn this study, we demonstrated that the EndMT markers were positively associated with MVD indicating unfavorable prognosis of invasive ductal carcinoma (IDC) patients. Functionally, TGF-β1 promoted migration, proliferation and angiogenesis of differentiated-endothelial BCSLCs by inducing EndMT in vitro and promoted tumor growth and angiogenesis in vivo. Mechanically, we revealed TGF-β1 induced EndMT by activation of TGF-β and Notch signaling pathways with increase of p-Smad2/3 and Notch1 expression. Moreover, we found Snail and Slug were key factors of TGF-β and Notch signaling pathways.ConclusionOur findings elucidated the mechanism of TGF-β1 in the promotion of angiogenesis and progression by EndMT in breast cancer.
A delta-radiomics model for preoperative prediction of invasive lung adenocarcinomas manifesting as radiological part-solid nodules
Authors: Chen Wufei , Wang Ruizhi , Ma Zhuangxuan , Hua Yanqing , Mao Dingbiao , Wu Hao , Yang Yuling , Li Cheng , Li Ming 
Published: November 2022

PurposeThis study aims to explore the value of the delta-radiomics (DelRADx) model in predicting the invasiveness of lung adenocarcinoma manifesting as radiological part-solid nodules (PSNs).MethodsA total of 299 PSNs histopathologically confirmed as lung adenocarcinoma (training set, n = 209; validation set, n = 90) in our hospital were retrospectively analyzed from January 2017 to December 2021. All patients underwent diagnostic noncontrast-enhanced CT (NCECT) and contrast-enhanced CT (CECT) before surgery. After image preprocessing and ROI segmentation, 740 radiomic features were extracted from NCECT and CECT, respectively, resulting in 740 DelRADx. A DelRADx model was constructed using the least absolute shrinkage and selection operator logistic (LASSO-logistic) algorithm based on the training cohort. The conventional radiomics model based on NCECT was also constructed following the same process for comparison purposes. The prediction performance was assessed using area under the ROC curve (AUC). To provide an easy-to-use tool, a radiomics-based integrated nomogram was constructed and evaluated by integrated discrimination increment (IDI), calibration curves, decision curve analysis (DCA), and clinical impact plot.ResultsThe DelRADx signature, which consisted of nine robust selected features, showed significant differences between the AIS/MIA group and IAC group (p &lt; 0.05) in both training and validation sets. The DelRADx signature showed a significantly higher AUC (0.902) compared to the conventional radiomics model based on NCECT (AUC = 0.856) in the validation set. The IDI was significant at 0.0769 for the integrated nomogram compared with the DelRADx signature. The calibration curve of the integrated nomogram demonstrated favorable agreement both in the training set and validation set with a mean absolute error of 0.001 and 0.019, respectively. Decision curve analysis and clinical impact plot indicated that if the threshold probability was within 90%, the integrated nomogram showed a high clinical application value.ConclusionThe DelRADx method has the potential to assist doctors in predicting the invasiveness for patients with PSNs. The integrated nomogram incorporating the DelRADx signature with the radiographic features could facilitate the performance and serve as an alternative way for determining management.
Real-world patterns of opioid therapy initiation in Spain, 2012–2018: A population-based, retrospective cohort study with 957,080 patients and 1,509,488 initiations
Authors: Hurtado Isabel , Robles Celia , Peiró Salvador , García-Sempere Aníbal , Llopis-Cardona Fran , Sánchez-Sáez Francisco , Rodríguez-Bernal Clara , Sanfélix-Gimeno Gabriel 
Published: November 2022

Introduction: Europe has seen a steady increase in the use of prescription opioids, especially in non-cancer indications. Epidemiological data on the patterns of use of opioids is required to optimize prescription. We aim to describe the patterns of opioid therapy initiation for non-cancer pain and characteristics of patients treated in a region with five million inhabitants in the period 2012 to 2018.Methods: Population-based retrospective cohort study of all adult patients initiating opioid therapy for non-cancer pain in the region of Valencia. We described patient characteristics at baseline and the characteristics of baseline and subsequent treatment initiation. We used multinominal regression models to identify individual factors associated with initiation.Results: A total of 957,080 patients initiated 1,509,488 opioid treatments (957,080 baseline initiations, 552,408 subsequent initiations). For baseline initiations, 738,749 were with tramadol (77.19%), 157,098 with codeine (16.41%) 58,436 (6.11%) with long-acting opioids, 1,518 (0.16%) with short-acting opioids and 1,279 (0.13%) with ultrafast drugs. When compared to tramadol, patients initiating with short-acting, long-acting and ultrafast opioids were more likely to be older and had more comorbidities, whereas initiators with codeine were more prone to be healthier and younger. Treatments lasting less than 7 days accounted for 41.82% of initiations, and 11.89% lasted more than 30 days. 19.55% of initiators with ultrafast fentanyl received more than 120 daily Morphine Milligram Equivalents (MME), and 16.12% of patients initiating with long-acting opioids were prescribed more than 90 daily MME (p &lt; 0.001). Musculoskeletal indications accounted for 65.05% of opioid use. Overlap with benzodiazepines was observed in 24.73% of initiations, overlap with gabapentinoids was present in 11.04% of initiations with long-acting opioids and 28.39% of initiators with short-acting opioids used antipsychotics concomitantly. In subsequent initiations, 55.48% of treatments included three or more prescriptions (vs. 17.60% in baseline initiations) and risk of overlap was also increased.Conclusion: Opioids are initiated for a vast array of non-oncological indications, and, despite clinical guidelines, short-acting opioids are used marginally, and a significant number of patients is exposed to potentially high-risk patterns of initiation, such as treatments lasting more than 14 days, treatments surpassing 50 daily MMEs, initiating with long-acting opioids, or hazardous overlapping with other therapies.
Economic evaluation of tislelizumab versus chemotherapy as second-line treatment for advanced or metastatic esophageal squamous cell carcinoma in China
Authors: Shi Fenghao , He Zixuan , Su Hang , Wang Lin , Han Sheng 
Published: November 2022

Background and purpose: The latest RATIONALE-302 trial (NCT03430843) showed that tislelizumab therapy significantly improved overall survival benefits for patients with advanced or metastatic esophageal squamous cell carcinoma (ESCC) compared with traditional chemotherapy. This study aimed to compare the cost-effectiveness of tislelizumab versus chemotherapy as a second-line treatment for advanced or metastatic ESCC in China.Methods: A partitioned survival model was developed to predict patients’ lifetime quality-adjusted life-years (QALYs) and incremental cost-effectiveness ratio (ICER) from the Chinese healthcare payers’ perspective. We extracted efficacy and safety data from the RATIONALE-302 trial and the local cost and resource use data from online databases and published studies. One-way sensitivity analysis (OWSA) and probabilistic sensitivity analysis (PSA) were performed to explore model uncertainty.Results: Compared with chemotherapy, tislelizumab generated a higher cost (US$ 10211.78 vs. US$ 7294.72) but yielded more QALY (0.78 vs. 0.51 QALYs). The ICER for tislelizumab was US$11073.85 per QALY gained. The PSA results indicated that the probability of tislelizumab being economical was 76% under a willingness-to-pay (WTP) threshold of 1.5 times per capita GDP ($17915) in China.Conclusion: Tislelizumab could be a promising cost-effective strategy as the second-line treatment for patients with ESCC compared with chemotherapy in the Chinese setting.
Efficacy evaluation of pulmonary hypertension therapy in patients with portal pulmonary hypertension: A systematic review and meta-analysis
Authors: Zhang Ruihua , Li Tengfei , Shao Yueming , Bai Wei , Wen Xiaoyu 
Published: November 2022

Objective: To determine the therapeutic effect of pulmonary arterial hypertension (PAH) agents for portal pulmonary hypertension (POPH).Design: Systematic review and meta-analysis.Background: POPH is a serious complication of end-stage liver disease with a low survival rate. Liver transplantation (LT) is an effective treatment. Due to the presence of POPH, some patients cannot undergo LT. After PAH treatment, patients with POPH can obtain good hemodynamics and cardiac function for LT, but there are no standard guidelines.Methods: Two independent researchers searched PubMed, EMBASE, Cochrane Library, and Web of Science for studies published from inception to 27 September 2022, focusing on the changes in hemodynamics and cardiac function in all patients with POPH to understand the effect of PAH treatment on the entire population of POPH patients. Among these, we specifically analyzed the changes in hemodynamics and cardiac function in moderate and severe POPH patients. After collecting the relevant data, a meta-analysis was carried out using the R program meta-package.Results: A total of 2,775 literatures were retrieved, and 24 literatures were included. The results showed that in all POPH patients (n = 1,046), the following indicators were significantly improved with PAH agents: mPAP: (MD = −9.11 mmHg, p &lt; 0.0001); PVR: (MD = −239.33 dyn·s·cm−5, p &lt; 0.0001); CO: (MD = 1.71 L/min, p &lt; 0.0001); cardiac index: (MD = 0.87 L/(min·m2), p &lt; 0.0001); 6MWD: (MD = 43.41 m, p &lt; 0.0001). In patients with moderate to severe POPH (n = 235), the following indicators improved significantly with PAH agents: mPAP (MD = −9.63 mmHg, p &lt; 0.0001); PVR (MD = −259.78 dyn·s·cm−5, p &lt; 0.0001); CO (MD = 1.76 L/min, p &lt; 0.0001); Cardiac index: (MD = 1.01 L/(min·m2), p = 0.0027); 6MWD: (MD = 61.30 m, p &lt; 0.0001).Conclusion: The application of PAH agents can improve cardiopulmonary hemodynamics and cardiac function in patients with POPH, especially in patients with moderate to severe POPH, and the above changes are more positive.Systematic Review Registration:https://inplasy.com, identifier INPLASY202250034.
Microwave-induced thermoacoustic imaging for the early detection of canine intracerebral hemorrhage
Authors: Li Jiawu , Wu Zhenru , Peng Chihan , Song Ling , Luo Yan 
Published: November 2022

Purpose: This study aimed to investigate the feasibility and validation of microwave-induced thermoacoustic imaging (TAI) for the early detection of canine intracerebral hemorrhage.Methods: A TAI system was used to record the thermoacoustic signal (TAS) of canine intracerebral hemorrhage in the study. First, the difference in TAS between deionized water, fresh ex vivo porcine blood and brain tissue was explored. Second, the canine hemorrhagic stroke model was established, and canine brain ultrasound examination and TAI examination were performed before modeling and at 0.5 h, 1 h, 2 h, 3 h, 4 h, 4.5 h, 5 h and 6 h after modeling. Finally, pathology and ultrasound were used as the reference diagnoses to verify the accuracy of the thermoacoustic imaging data.Results: The results showed that significant differences were observed in TASs among deionized water, fresh ex vivo porcine blood and brain tissue. The intensity of the thermoacoustic signal of blood was significantly higher than that of ex vivo porcine brain tissue and deionized water. The intracerebral hemorrhage model of five beagles was successfully established. Hematomas presented hyperintensity in TAI. Considering ultrasound and pathology as reference diagnoses, TAI can be used to visualize canine intracerebral hemorrhage at 0.5 h, 1 h, 2 h, 3 h, 4 h, 4.5 h, 5 h and 6 h after modeling.Conclusion: This is the first experimental study to explore the use of TAI in the detection of intracerebral hemorrhage in large live animals (canine). The results indicated that TAI could detect canine intracerebral hemorrhage in the early stage and has the potential to be a rapid and noninvasive method for the detection of intracerebral hemorrhage in humans.
Foliar spray of silica improved water stress tolerance in rice (Oryza sativa L.) cultivars
Authors: El-Okkiah Samira A. F. , El-Afry Mohamed M. , Shehab Eldeen Safaa A. , El-Tahan Amira M. , Ibrahim Omar M. , Negm Mostafa M. , Alnafissa Mohamad , El-Saadony Mohamed T. , Almazrouei Hessa M. R. S. , AbuQamar Synan F. , El-Tarabily Khaled A. , Selim Dalia A. 
Published: November 2022

Rice (Oryza sativa L.) is a major cereal crop and a staple food across the world, mainly in developing countries. Drought is one of the most important limiting factors for rice production, which negatively affects food security worldwide. Silica enhances antioxidant activity and reduces oxidative damage in plants. The current study evaluated the efficiency of foliar spray of silica in alleviating water stress of three rice cultivars (Giza178, Sakha102, and Sakha107). The seedlings of the three cultivars were foliar sprayed with 200 or 400 mg l-1 silica under well-watered [80% water holding capacity (WHC)] and drought-stressed (40% WHC)] conditions for two summer seasons of 2019 and 2020. The obtained results demonstrated that drought stress caused significant decreases in growth, yield, and physiological parameters but increases in biochemical parameters (except proline) of leaves in all rice cultivars compared to well-irrigated plants (control). The roots of drought-stressed seedlings exhibited smaller diameters, fewer numbers, and narrower areas of xylem vessels compared to those well-watered. Regardless of its concentration, the application of silica was found to increase the contents of photosynthetic pigments and proline. Water relation also increased in seedlings of the three tested rice cultivars that were treated with silica in comparison to their corresponding control cultivars when no silica was sprayed. Foliar application of 400 mg l-1 silica improved the physiological and biochemical parameters and plant growth. Overall, foliar application of silica proved to be beneficial for mitigating drought stress in the tested rice cultivars, among which Giza178 was the most drought-tolerant cultivar. The integration of silica in breeding programs is recommended to improve the quality of yield and to provide drought-tolerant rice cultivars under drought-stress conditions.
Intergenerational support and depressive symptoms in old age: The difference between urban and rural China
Authors: Wang Chenxi , Liu Zhengkui , Chen Tianyong , Wang Jinfeng , Zhang Xin , Han Buxin 
Published: November 2022

ObjectivesIntergenerational support is associated with fewer depressive symptoms in old age. Uneven development has resulted in huge urban–rural disparities in China, which could lead to different intergenerational relationships. The present study aimed to examine whether intergenerational support was associated with depressive symptoms differently among urban and rural Chinese older participants.MethodsA sample of 3,498 participants from nine pairs of urban subdistricts and rural villages were included in the present study. Depressive symptoms were measured by the 10-item Center for Epidemiological Studies Depression Scale, and the intergenerational support mechanisms (financial, instrumental, and emotional) were assessed with a self-designed questionnaire.ResultsSignificant areas by support effect for depressive symptoms indicated different associations between intergenerational financial and emotional support and depressive symptoms in urban and rural areas. Specifically, urban older participants receiving emotional support from adult children and rural older participants receiving financial support from adult children showed fewer depressive symptoms. In both areas, participants receiving instrumental support showed fewer depressive symptoms.ConclusionOur study is the first to compare the urban–rural disparity in association between intergenerational support and depressive symptoms in a developing country, China. The results support modernization theories proposing weakened economic function but intensified emotional ties in societies with higher level of development. Communication-based intergenerational emotional support should be promoted in urban areas, and formal support systems should provide financial and instrumental support to the vulnerable rural older population.
Follow-up conditions of care and associated factors among adult hypertensive patients during COVID-19 at West Arsi public health facilities, Southeastern Ethiopia: A multi-center cross-sectional study
Authors: Hiko Ahmed , Assefa Nega , Ataro Zerihun , Sertsu Addisu , Yadeta Elias , Balcha Teganu , Amano Abdulkerim , Asfaw Henock , Dechasa Deribe Bekele , Nigussie Kabtamu , Abdisa Lemesa 
Published: November 2022

BackgroundCoronavirus disease (COVID-19) is a viral-borne infection caused by the SARS-CoV-2 virus. Aside from the morbidity and mortality effects, it leaves the majority of hypertensive patients untreated and vulnerable to uncontrolled hypertension.ObjectiveThe purpose of this study was to assess follow-up conditions of care and its associated factors among adult hypertensive patients during COVID-19 in public health facilities of West Arsi, Southeastern Ethiopia.MethodsA health facility-based retrospective cross-sectional study was conducted among 423 adult hypertensive patients in the West Arsi public health facilities from July 5 to August 6, 2021. A systematic random sampling technique was used to recruit the study participants. A pretested structured face-to-face interviewer and medical records were used to collect sociodemographic variables, basic clinical features, and follow-up data. The follow-up conditions of care were assessed using 12 items with “yes or no” questions. Then, based on the mean value of the items, the follow-up conditions of care were dichotomized into good and poor. As a result, the follow-up condition was good if the score was greater or equal to the mean, and poor unless otherwise. To investigate parameters related with follow-up conditions of care, bivariable and multivariable logistic regression analyses were used. A 95% confidence interval and a p-value of 0.05 were used to indicate a significant association.ResultsThe rate of poor follow-up conditions of care during COVID-19 was 29% (95% confidence interval: 24.9–33.4%), according to this study. Age ≥ 60 years (AOR = 3.55; 95% CI: 2.09–6.03), transportation problem (AOR = 2.43; 95% CI: 1.28–4.61), fear of COVID-19 (AOR = 3.34; 95% CI: 1.59–7.01), co-morbidity (AOR = 1.93; 95% CI: 1.14–3.26) and physical distancing (AOR = 2.43; 95% CI: 1.44–4.12) were significantly associated with poor follow-up conditions of care.ConclusionsIn our study, 29% of the participants had poor follow-up care conditions. When compared to WHO recommendations, the findings of this study may explain poor follow-up care conditions. To enhance patients' follow-up treatment, evidence-based target interventions should be designed and executed, taking into account individuals at high risks, such as those over the age of 60 and those with comorbidities, and identifying additional risk factors.
Aerosol formation during processing of potentially infectious samples on Roche immunochemistry analyzers (cobas e analyzers) and in an end-to-end laboratory workflow to model SARS-CoV-2 infection risk for laboratory operators
Authors: Burghardt Géza V. , Eckl Markus , Huether Doris , Larbolette Oliver H. D. , Lo Faso Alessia , Ofenloch-Haehnle Beatus R. , Riesch Marlene A. , Herb Rolf A. 
Published: November 2022

ObjectivesTo assess aerosol formation during processing of model samples in a simulated real-world laboratory setting, then apply these findings to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to assess the risk of infection to laboratory operators.DesignThis study assessed aerosol formation when using cobas e analyzers only and in an end-to-end laboratory workflow. Recombinant hepatitis B surface antigen (HBsAg) was used as a surrogate marker for infectious SARS-CoV-2 viral particles. Using the HBsAg model, air sampling was performed at different positions around the cobas e analyzers and in four scenarios reflecting critical handling and/or transport locations in an end-to-end laboratory workflow. Aerosol formation of HBsAg was quantified using the Elecsys® HBsAg II quant II immunoassay. The model was then applied to SARS-CoV-2.ResultsFollowing application to SARS-CoV-2, mean HBsAg uptake/hour was 1.9 viral particles across the cobas e analyzers and 0.87 viral particles across all tested scenarios in an end-to-end laboratory workflow, corresponding to a maximum inhalation rate of &lt;16 viral particles during an 8-hour shift.ConclusionLow production of marker-containing aerosol when using cobas e analyzers and in an end-to-end laboratory workflow is consistent with a remote risk of laboratory-acquired SARS-CoV-2 infection for laboratory operators.