bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs
Authors: Schermuly Isabel I. , Romanet Stella , Klünemann Martina , Mastrototaro Lucia , Pieper Robert , Zentek Jürgen , Whelan Rose A. , Aschenbach Jörg R. 
Published: March 2022

AbstractMethionine (Met) as an essential amino acid has key importance in a variety of metabolic pathways. This study investigated the influence of three dietary Met supplements (0.21% L-Met, 0.21% DL-Met and 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA)) on the metabolome and inflammatory status in the small intestine of pigs. Epithelia from duodenum, proximal jejunum, middle jejunum and ileum were subjected to metabolomics analysis and qRT-PCR of caspase 1, NLR family pyrin domain containing 3 (NLRP3), interleukins IL1β, IL8, IL18, and transforming growth factor TGFβ. Principal component analysis of the intraepithelial metabolome revealed strong clustering of samples by intestinal segment but not by dietary treatment. However, pathway enrichment analysis revealed that after L-Met supplementation polyunsaturated fatty acids (PUFA) and tocopherol metabolites were lower across small intestinal segments, whereas monohydroxy fatty acids were increased in distal small intestine. Pigs supplemented with DL-HMTBA showed a pronounced shift of secondary bile acids (BA) and sphingosine metabolites from middle jejunum to ileum. In the amino acid super pathway, only histidine metabolism tended to be altered in DL-Met-supplemented pigs. Diet did not affect the expression of inflammation-related genes. These findings suggest that dietary supplementation of young pigs with different Met sources selectively alters lipid metabolism without consequences for inflammatory status.
Chronic obstructive pulmonary disease and influenza vaccination effect in preventing outpatient and inpatient influenza cases
Authors: Martínez-Baz Iván , Casado Itziar , Navascués Ana , Portillo María Eugenia , Guevara Marcela , Ezpeleta Carmen , Castilla Jesús 
Published: March 2022

AbstractEvidence of influenza vaccine effectiveness in preventing confirmed influenza among persons diagnosed with chronic obstructive pulmonary disease (COPD) is scarce. We assessed the average effect of influenza vaccination in the current and prior seasons in preventing laboratory-confirmed influenza in COPD patients. We carried out a pooled test-negative case–control design in COPD patients hospitalized or presented to primary healthcare centres with influenza-like illness who were tested for influenza in 2015/2016 to 2019/2020 seasons in Navarre, Spain. Influenza vaccination status in the current and 5 prior seasons was compared between confirmed-influenza cases and test-negative controls. Vaccination effect was compared between target patients for vaccination with and without COPD. Out of 1761 COPD patients tested, 542 (31%) were confirmed for influenza and 1219 were test-negative controls. Average effect for current-season vaccination in preventing influenza was 40% (95% CI 20–54%), and for vaccination in prior seasons only was 24% (95% CI –10 to 47%). Point estimates seemed higher in preventing outpatient cases (60% and 58%, respectively) than inpatient cases (37% and 19%, respectively), but differences were no statistically significant. Influenza vaccination effect was similar in target population with and without COPD (p = 0.339). Influenza vaccination coverage in control patients with COPD was 68.3%. A 13.7% of the influenza cases in patients with COPD could be prevented by extending the influenza vaccine coverage. Average effect of current-season influenza vaccination was moderate to prevent influenza in COPD persons. The increase of influenza vaccination coverage can still prevent COPD exacerbations.
Er:YAG laser-induced cavitation can activate irrigation for the removal of intraradicular biofilm
Authors: Nagahashi Taiji , Yahata Yoshio , Handa Keisuke , Nakano Masato , Suzuki Shigeto , Kakiuchi Yusuke , Tanaka Toshinori , Kanehira Masafumi , Suresh Venkataiah Venkata , Saito Masahiro 
Published: March 2022

AbstractWe investigated the biofilm removal effects of laser activated irrigation (LAI) using a pig model, focusing on the impact of the fiber tip position, and used a high-speed camera to observe the occurrence and positioning of the cavitation associated with laser irradiation. A total of 16 roots of deciduous mandibular second premolars from 4 pigs were used. After a pulpectomy, the canals were left open for 2 weeks and sealed for 4 weeks to induce intraradicular biofilm. Root canal irrigation was then performed with Er:YAG laser activation. The fiber tip was inserted at two different positions, i.e., into the root canal in the intracanal LAI group and into the pulp chamber in the coronal LAI group. Intracanal needle irrigation with saline or 5% NaOCl was utilized in the positive control and conventional needle irrigation (CNI) groups. SEM and qPCR were carried out to evaluate treatment efficacy. Statistical analysis was performed using ANOVA and a Tukey–Kramer post-hoc test for qPCR and with a Steel–Dwass test to compare the SEM scores, with α = 0.05. A high-speed camera was used to observe the generation of cavitation bubbles and the movement of the induced bubbles after laser irradiation. The intracanal and coronal LAI groups showed significantly lower amounts of bacteria than either the positive control or CNI groups. There was no significant difference found between the intracanal and coronal LAI groups. SEM images revealed opened dentinal tubules with the destruction of biofilm in both LAI groups. High-speed camera images demonstrated cavitation bubble production inside the root canal after a single pulse irradiation pulse. The generated bubbles moved throughout the entire internal multi-rooted tooth space. Coronal LAI can generate cavitation in the root canal with a simply placed fiber inside the pulp chamber, leading to effective biofilm removal. This method could thus contribute to the future development of endodontic treatments for refractory apical periodontitis caused by intraradicular biofilm.
Concentration-dependent dual effects of exogenous sucrose on nitrogen metabolism in Andrographis paniculata
Authors: Huang Xue-Jing , Jian Shao-Fen , Chen Dong-Liang , Zhong Chu , Miao Jian-Hua 
Published: March 2022

AbstractThe effects of exogenous sucrose (Suc) concentrations (0, 0.5, 1, 5, 10 mmol L−1) on carbon (C) and nitrogen (N) metabolisms were investigated in a medicinal plant Andrographis paniculata (Chuanxinlian). Suc application with the concentration of 0.5–5 mmol L−1 significantly promoted plant growth. In contrast, 10 mmol L−1 Suc retarded plant growth and increased contents of anthocyanin and MDA and activity of SOD in comparison to 0.5–5 mmol L−1 Suc. Suc application increased contents of leaf soluble sugar, reducing sugar and trerhalose, as well as isocitrate dehydrogenase (ICDH) activity, increasing supply of C-skeleton for N assimilation. However, total leaf N was peaked at 1 mmol L−1 Suc, which was consistent with root activity, suggesting that exogenous Suc enhanced root N uptake. At 10 mmol L−1 Suc, total leaf N and activities of glutamine synthase (GS), glutamate synthase (GOGAT), NADH-dependent glutamate dehydrogenase (NADH-GDH) and glutamic–pyruvic transaminase (GPT) were strongly reduced but NH4+ concentration was significantly increased. The results revealed that exogenous Suc is an effective stimulant for A. paniculata plant growth. Low Suc concentration (e.g. 1 mmol L−1) increased supply of C-skeleton and promoted N uptake and assimilation in A. paniculata plant, whereas high Suc concentration (e.g. 10 mmol L−1) uncoupled C and N metabolisms, reduced N metabolism and induced plant senescence.
Molecular characterization of strawberry vein banding virus from China and the development of loop‑mediated isothermal amplification assays for their detection
Authors: Ren Junda , Zhang Jiaxing , Wang Qiushi , Zhou Yu , Wang Jingxuan , Ran Ce , Shang Qiaoxia 
Published: March 2022

AbstractStrawberry vein banding virus (SVBV) is one of the serious viral pathogens infecting strawberry worldwide. To understand the molecular characterization of SVBV from China, complete genome sequences of sixteen SVBV isolates were cloned and sequenced. Sequence comparison showed they shared high nucleotide sequence identity (93.6–99.5%) with isolates from China and Japan (96.6–98.4%), while relatively low identity with the isolates from Canada (91.9–93.7%) and USA (85.5–85.9%). Phylogenetic analyses based on the complete genome sequence or coat protein (CP) gene showed the SVBV isolates clustered into three clades correlated with geographic distribution. Recombination analyses identified 13 recombinants and 21 recombinant events, indicating frequent and multiple recombinations in SVBV evolution. Furthermore, a sensitive loop-mediated isothermal amplification (LAMP) method was developed for rapid detection of SVBV isolates, which could be especially suitable for seedling propagation, virus-free culture and routine diagnostics in field investigation. This study offers new understanding of the molecular evolution and may help to improve the management of SVBV.
Genotype–phenotype correlation in patients with deletional and nondeletional mutations of Hb H disease in Southwest of Iran
Authors: Hamid Mohammad , keikhaei Bijan , Galehdari Hamid , Saberi Alihossein , Sedaghat Alireza , Shariati Gholamreza , Mohammadi-Anaei Marziye 
Published: March 2022

AbstractWe studied the alpha-globin gene genotypes, hematologic values, and transfusion-dependence of patients with Hb H disease. Molecular characterization of alpha-thalassemia was performed. We identified 120 patients with Hb H disease. Of these patients, 35 (29.16%) had deletional form of Hb H disease, and 85 (70.83%) had different form of non-deletional Hb H disease. The most frequently observed Hb H genotypes were --Med/–α3.7in 33 patients (27.5%), αCD19(-G)α/αCD19(-G)α in 25 cases (20.83%), αpolyA2α/αpolyA2α in 15 (12.5%), and αpolyA1α/αpolyA1α in 13 (10.83%) respectively. The probability of receiving at least one transfusion blood in deletional form was observed in 3 of 35 (8.57%) patients which just seen in 3 of 33 (9%) patients with --Med/–α3.7genotype. This form was also observed in 8 of 85 (9.4%) patients in non-deletional Hb H diseases which five of them had Med deletion in compound with alpha globin point mutations. Nondeletional Hb H disease was more severe than deletional Hb H disease requiring more blood transfusions. We can recommend that Med deletion in compound with alpha-globin point mutations, polyA1 and constant spring in homozygous form needs to be taken into consideration when offering counseling to high-risk couples.
Dimensions and forms of artefacts in 1.5 T and 3 T MRI caused by cochlear implants
Authors: Gottfried Timo M. , Dejaco Daniel , Fischer Natalie , Innerhofer Veronika , Chacko Lejo Johnson , Widmann Gerlig , Kremser Christian , Riechelmann Herbert , Schmutzhard Joachim 
Published: March 2022

AbstractCochlear implantation is a standard treatment option due to expanding indications. Cranial magnetic resonance imaging (cMRI) has become a widespread diagnostic tool. Therefore, an increased number of cochlear implant (CI) users are undergoing cMRI scans. This study aimed to investigate the issue of the CI magnet impacting MRI quality and artifacts. 1.5 T and 3 T MRI scans with 4 defined sequences (T2-TSE, T2-TIRM, T1-3D-MPRAGE, and TDI) were performed on a phantom with a CI (SYNCHRONY System by MED-EL Austria) in place. The resulting MRI artifacts were retrospectively compared to MRI artifacts observed in patients with a CI. All images were transferred to AMIRA and visualized by manual segmentation. Usable image quality was achieved in three sequences (T2-TSE, T2-TIRM and T1-mprage). Observed artifacts differed in shape and size depending on the sequence. Maximum diameters of signal void areas ranged from 58 × 108 × 98 mm to 127 × 123 × 153 mm. Image distortions were larger. MRI artifacts caused by the SYNCHRONY system are asymmetric with varying shape, depending on the sequence. The phantom artefacts are similar to those in CI users. Considering the observed asymmetry, the hypothesis of varying implantation locations resulting in varying positions of the signal void area needs to be further investigated.
Serum metabolomic responses to aerobic exercise in rats under chronic unpredictable mild stress
Authors: Liu Xiangyu , Han Yumei , Zhou Shi , Tian Junsheng , Qin Xuemei , Ji Cui , Zhao Weidi , Chen Anping 
Published: March 2022

AbstractThis study analyzed the effects of aerobic exercise on endogenous serum metabolites in response to chronic unpredictable mild stress (CUMS) using a rat model, aiming to identify the metabolic regulatory pathways involved in the antidepressant effect resulted from a 28-day treadmill aerobic exercise intervention. The animals were randomly divided into four groups (n = 8): normal control, normal with aerobic exercise, CUMS control, and CUMS with aerobic exercise. Body weight, sucrose preference and open field tests were performed weekly during the intervention period for changes in depressant symptoms. Serum metabolic profiles obtained by using the LC–MS/MS metabolomics were analyzed to explore the regulatory mechanism for the effect of the aerobic exercise on depression. Behavior tests showed that the aerobic exercise resulted in a significant improvement in depression-like behavior in the CUMS rats. A total of 21 differential metabolites were identified as being associated with depression in serum metabolic profile, of which the aerobic exercise significantly modulated 15, mainly related to amino acid metabolism and energy metabolism. Collectively, this is the first study that LC–MS/MS techniques were used to reveal the modulatory effects of aerobic exercise on the serum metabolic profile of depressed rats and the findings further enriched our understanding of potential mechanisms of aerobic exercise interventions on depression.
Pre-activation of autophagy impacts response to olaparib in prostate cancer cells
Authors: Cahuzac Maxime , Langlois Patricia , Péant Benjamin , Fleury Hubert , Mes-Masson Anne-Marie , Saad Fred 
Published: March 2022

AbstractPoly (ADP-ribose) polymerase 1 (PARP1) plays an essential role in DNA repair and is targeted by anticancer therapies using PARP inhibitors (PARPi) such as olaparib. PARPi treatment in prostate cancer (PC) is currently used as a monotherapy or in combination with standard therapies (hormonotherapy) in clinical trials for patients with DNA damage response mutation. Unfortunately, 20% of these patients did not respond to this new treatment. This resistance mechanism in PC is still not well understood. Here, we report that autophagy affects differently the response of PC cell lines to olaparib depending on its activation status. Pre-activation of autophagy before olaparib resulted in an increase of DNA repair activity by homologous recombination (HR) to repair double-strand breaks induced by olaparib and enhanced cell proliferation. When autophagy was activated after olaparib treatment, or completely inhibited, PC cells demonstrated an increased sensitivity to this PARPi. This autophagy-mediated resistance is, in part, regulated by the nuclear localization of sequestrosome 1 (SQSTM1/p62). Decrease of SQSTM1/p62 nuclear localization due to autophagy pre-activation leads to an increase of filamin A (FLNA) protein expression and BRCA1/Rad51 recruitment involved in the HR pathway. Our results reveal that autophagy basal levels may in part determine amenability to PARPi treatment.
Autonomous design of new chemical reactions using a variational autoencoder
Authors: Tempke Robert , Musho Terence 
Published: March 2022

AbstractArtificial intelligence based chemistry models are a promising method of exploring chemical reaction design spaces. However, training datasets based on experimental synthesis are typically reported only for the optimal synthesis reactions. This leads to an inherited bias in the model predictions. Therefore, robust datasets that span the entirety of the solution space are necessary to remove inherited bias and permit complete training of the space. In this study, an artificial intelligence model based on a Variational AutoEncoder (VAE) has been developed and investigated to synthetically generate continuous datasets. The approach involves sampling the latent space to generate new chemical reactions. This developed technique is demonstrated by generating over 7,000,000 new reactions from a training dataset containing only 7,000 reactions. The generated reactions include molecular species that are larger and more diverse than the training set.
Nickel-catalyzed alkyl-arylation of 3,3,3-trifluoropropene
Authors: Xu Chang , Wang Ming-Kuan , Zhang Shu , Zhang Xingang 
Published: March 2022

AbstractOwing to the versatile synthetic utility of its carbon-carbon double bond, low-cost industrial chemical 3,3,3-trifluoropropene (TFP) represents one of the most straightforward and cost-efficient precursors to prepare trifluoromethylated compounds. However, only limited methods for the efficient transformations of TFP have been reported so far. Here, we report a nickel-catalyzed dicarbofunctionalization of TFP. The reaction uses inexpensive NiCl2·6H2O as the catalyst and 4,4’-biMeO-bpy and PCy2Ph as the ligands, allowing the alkyl-arylation of TFP with a variety of tertiary alkyl iodides and arylzinc reagents in high efficiency. This nickel-catalyzed process overcomes the previous challenges by suppressing β-H and β-F eliminations from TFP, rendering this strategy effective for the transformations of TFP into medicinal interest trifluoromethylated compounds.
Towards entanglement distillation between atomic ensembles using high-fidelity spin operations
Authors: Liu Chao , Tu Tao , Li Pei-Yun , Liu Xiao , Zhu Xing-Yu , Zhou Zong-Quan , Li Chuan-Feng , Guo Guang-Can 
Published: March 2022

AbstractEntanglement distillation is an essential ingredient for long-distance quantum communication. However, owing to their demanding requirements, integrating such entanglement distillation processing in scalable quantum devices remains an outstanding challenge. Here we propose the implementation of the filtering protocol in atomic ensembles, which are promising candidates for building quantum repeater nodes, and analyze the boost entanglement distribution rate considering different scenarios. Moreover, we demonstrate the key step of this approach with a proof-of-principle experiment in a rare-earth-ion-doped crystal (143Nd3+:Y2SiO5). Leveraging its multi-level structure and long-lived coherence, spin manipulations are implemented with an average fidelity exceeding 97.2%, leading to the preparation of entanglement between the electron and nuclear spins with a concurrence of 0.75 with a sample temperature of 100 mK. The versatility, robustness, and potential scalability of our proposal contribute to the construction of quantum repeaters and quantum networks based on atomic ensembles.
A recyclable covalent triazine framework-supported iridium(iii) terpyridine complex for the acceptorless dehydrogenative coupling of o-aminobenzyl alcohols with ketones to form quinolines
Authors: Chen Xiaozhong , Ai Yao , Liu Deyun , Liu Peng , Xu Xiangchao , Yang Jiazhi , Li Feng 
Published: March 2022

A complex Ir(tpy)@CTF, which is synthesized by the coordinative immobilization of [Ir(tpy)Cl3] on a covalent triazine framework, was proven to be a recycle catalyst for the acceptorless dehydrogenative coupling of o-aminobenzyl alcohols with ketones.
Mn(iii)-Catalyzed cascade cyclization reaction ofo-acyl aromatic isocyanides with boronic acids
Authors: Liu Jingya , Li Lei , Bu Xiubin , Yuan Yu , Wang Xin , Sun Ran , Zhou Ming-Dong , Wang He 
Published: March 2022

A Mn(iii)-catalyzed cascade cyclization of o-acyl aromatic isocyanides with boronic acids was examined to give a series of 3-hydroxyindolenines in single-step. This cascade process involved a transmetalation/nucleophilic addition/intramolecular cyclization sequence.
Cell models for Down syndrome-Alzheimer’s disease research
Authors: Wu Yixing , West Nicole R. , Bhattacharyya Anita , Wiseman Frances K. 
Published: March 2022

Abstract
Down syndrome (DS) is the most common chromosomal abnormality and leads to intellectual disability, increased risk of cardiac defects, and an altered immune response. Individuals with DS have an extra full or partial copy of chromosome 21 (trisomy 21) and are more likely to develop early-onset Alzheimer’s disease (AD) than the general population. Changes in expression of human chromosome 21 (Hsa21)-encoded genes, such as amyloid precursor protein (APP), play an important role in the pathogenesis of AD in DS (DS-AD). However, the mechanisms of DS-AD remain poorly understood. To date, several mouse models with an extra copy of genes syntenic to Hsa21 have been developed to characterise DS-AD-related phenotypes. Nonetheless, due to genetic and physiological differences between mouse and human, mouse models cannot faithfully recapitulate all features of DS-AD. Cells differentiated from human-induced pluripotent stem cells (iPSCs), isolated from individuals with genetic diseases, can be used to model disease-related cellular and molecular pathologies, including DS. In this review, we will discuss the limitations of mouse models of DS and how these can be addressed using recent advancements in modelling DS using human iPSCs and iPSC-mouse chimeras, and potential applications of iPSCs in preclinical studies for DS-AD.
Lipreading: A Review of Its Continuing Importance for Speech Recognition With an Acquired Hearing Loss and Possibilities for Effective Training
Authors: Bernstein Lynne E. , Jordan Nicole , Auer Edward T. , Eberhardt Silvio P. 
Published: March 2022

Purpose:
The goal of this review article is to reinvigorate interest in lipreading and lipreading training for adults with acquired hearing loss. Most adults benefit from being able to see the talker when speech is degraded; however, the effect size is related to their lipreading ability, which is typically poor in adults who have experienced normal hearing through most of their lives. Lipreading training has been viewed as a possible avenue for rehabilitation of adults with an acquired hearing loss, but most training approaches have not been particularly successful. Here, we describe lipreading and theoretically motivated approaches to its training, as well as examples of successful training paradigms. We discuss some extensions to auditory-only (AO) and audiovisual (AV) speech recognition.

Method:
Visual speech perception and word recognition are described. Traditional and contemporary views of training and perceptual learning are outlined. We focus on the roles of external and internal feedback and the training task in perceptual learning, and we describe results of lipreading training experiments.

Results:
Lipreading is commonly characterized as limited to viseme perception. However, evidence demonstrates subvisemic perception of visual phonetic information. Lipreading words also relies on lexical constraints, not unlike auditory spoken word recognition. Lipreading has been shown to be difficult to improve through training, but under specific feedback and task conditions, training can be successful, and learning can generalize to untrained materials, including AV sentence stimuli in noise. The results on lipreading have implications for AO and AV training and for use of acoustically processed speech in face-to-face communication.

Conclusion:
Given its importance for speech recognition with a hearing loss, we suggest that the research and clinical communities integrate lipreading in their efforts to improve speech recognition in adults with acquired hearing loss.