bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microneedle, a one-plane bevel-tipped fabrication by 3D printing process.
Authors: Villota Isabella , Calvo Paulo C. , Campo Oscar I. , Rico Faruk Fonthal 
Published: April 2022

Abstract
This article presents the microneedles analysis where the design parameters studied were length, inner and outer diameter range. The ANSYS software was used to analyze the mechanical behavior of the microneedles. Following this, the range of inner and outer diameters were completed by mechanical simulations, getting from 30 μm to 134 μm, as the inner diameter range and 208 μm to 250 μm as the outer diameter range. With these ranges, a mathematical model was made using fourth-order polynomial regression, with a correlation of 0,9993. This mathematical model generalizes the relationship of the inner diameter to the outer diameter within the ranges, ensuring a safety factor of four, where Von Misses forces of the microneedle are around 17.931 MPa. In addition, the microneedle concept was made by 3D printing using a biocompatible resin of class 1. The features presented by the microneedle designed in this work make it a promising option to be implemented in a transdermal drug delivery device.
Experimental climate change impacts on Baltic coastal wetland plant communities
Authors: Bergamo Thaísa F. , Ward Raymond D. , Joyce Christopher B. , Villoslada Miguel , Sepp Kalev 
Published: April 2022

Abstract
Coastal wetlands provide a range of important ecosystem services, yet they are under threat from a range of stressors including climate change. This is predominantly as a result of alterations to the hydroregime and associated edaphic factors. We used a three year mesocosm experiment to assess changes in coastal plant community composition for three plant communities in response to altered water level and salinity scenarios. Species richness and abundance were calculated by year and abundance was plotted using rank abundance curves. The permutational multivariate analysis of variance with Bray-Curtis dissimilarity was used to examine differences among treatments in plant community composition. A principal coordinate analysis was used to visualize the responses of communities to treatments by year. Results showed that all three plant communities responded differently to altered water levels and salinity. Species richness and abundance increased significantly in an Open Pioneer while Lower and Upper Shore plant communities showed less change. Species abundances changed in all plant communities with shifts in species composition significantly influenced by temporal and treatment. The observed responses to experimentally altered conditions highlight the need for conservation of these important ecosystems in the face of predicted climate change.
Cuspal deflection and marginal microleakage following the application of bulk-fill and conventional composite resins
Authors: kasraei shahin , ebrahimizadeh hosna , sanjari khashayar 
Published: April 2022

Abstract
Objectives
This study compared the cuspal deflection and marginal microleakage following the application of bulk-fill and conventional composite resins.
Materials and Methods
70 sound premolars with MOD preparation were randomized into 5 groups (n = 14). In three groups, the cavities were restored with Tetric Ceram N, X-tra fil, and Opus bulk-fill composite resins. In the remaining two groups, the cavities were restored with P60 conventional composite with 2 mm (incremental technique) and 4 mm (bulk-fill technique) increments. Cuspal deflection was measured by a digital micrometer. After aging, marginal microleakage was assessed. Data were analyzed by repeated measures one-way and two-way ANOVA, and Tukey, Bonferroni, Kruskal-Wallis, Mann-Whitney, Friedman, Wilcoxon signed rank, and t tests.
Results
Cuspal deflection of bulk-fill composite groups was lower than that of conventional composite applied incrementally (P < 0.05), but it was not significantly different among the bulk-fill composite groups (P = 0.16). The microleakage scores was not significantly different among the bulk-fill composites, or between the bulk-fill and conventional incremental composite group (P > 0.05). Microleakage at the cervical cementum margin of proximal box was significantly higher than that at the cervical and occlusal enamel margins of proximal box (P = 0.001).
Conclusion
the cuspal deflection in bulk-fill composite restoration was lower than that in the conventional composite in incremental technique but marginal microleakage of bulk-fill and conventional composite resin restorations were comparable.
Perceptions of COVID-19 threat in Uganda and its impact on household welfare: Evidence from the High-Frequency Phone Survey on COVID-19
Authors: James Mukoki , Faustino Byanyima , Bosco Oryema John 
Published: April 2022

Abstract
The novel coronavirus that emerged around late 2019 from the central province of Hubei in China in the city of Wuhan has greatly affected people who live around the world on a scale unreported before. The pandemic has affected millions of people and thousands have died ever since its discovery. Worse still besides the direct impacts of the disease such as cumulative infection and deaths reported around the world, the indirect effects such as strict containment measures of partial and full economic lockdowns have disrupted global supply chains, led to the loss of jobs due to massive layoffs and loss in government revenues (Mukiza, 2020). Despite impressive efforts towards the production of a vaccine with some being in approval stages, total control measures of COVID-19 in developing countries such as Uganda are still in their infancy phases and most likely may be unachievable. Therefore, this study aimed at examining the predictors of people’s perceptions of COVID-19 in Uganda, using the first phase of the Uganda, High-Frequency Phone Survey (UHPS, 2020).
Effects of gestational haloperidol exposure on mRNA expressions related to glutamine and GABA receptors in offspring
Authors: Iga Jun-ichi , Kumon Hiroshi , Yoshino Yuta , Funahashi Yu , Ochi Shinichiro , Ueno Shu-ichi 
Published: April 2022

Abstract
Objectives: The aim of the present study was to investigate the effects of intraperitoneal haloperidol (HAL) injection to pregnant mice on glutamine and GABA receptors in the brain of offspring mice. Method: Eight-week-old pregnant mice were treated with either intraperitoneal HAL or normal saline injection, and their offspring were defined as F1 mice. In addition, eight-week-old male mice were used as acute mice that were intraperitoneally injected with HAL or normal saline for 20 days. F1 and acute mice were sacrificed by decapitation, and their hippocampi, frontal lobes, and temporal lobes were collected. RNA was isolated, and mRNA expression levels were measured by RT-qPCR. Western blotting was performed using the frontal lobes of F1 mice.Results: In the hippocampi of F1 mice, Grik3 (p = 0.023) and Gabra3 (p = 0.004) mRNA expression levels were significantly higher in the HAL group than in the control group, whereas Gria2 (p < 0.001) and Grin2a (p < 0.001) mRNA expression levels were significantly lower in the HAL group than in the control group. Gria2 (p = 0.015), Grik3 (p = 0.037), and Grin2a (p = 0.012) mRNA expression levels were significantly lower in the HAL group than in the control group in the frontal lobes of F1 mice. In the hippocampi of acute mice, Grik3 (p = 0.049) and Gabra3 (p = 0.007) mRNA expressions were significantly increased in the HAL group.Conclusions: The present results may explain the effects of fetal exposure to antipsychotics on neurodevelopmental modifications and teratogenicity.
Large deformations of hyperelastic curved beams based on the absolute nodal coordinate formulation
Authors: Li Liang , Wang Yaolun , Guo Yongbin , Zhang Dingguo 
Published: April 2022

Abstract
Compared with traditional linear elastic materials, the soft structure composed of incompressible hyperelastic materials has not only geometrical nonlinearity but also material nonlinearity during deformation. In this paper, the absolute nodal coordinate formulation (ANCF) is used to study the large deformations and large overall motions of incompressible hyperelastic curved beams. A novel large deformation dynamic modeling method for curved beams made of hyperelastic materials is proposed, in which a simplified Neo-Hookean model is combined with the one-dimensional ANCF beam element. The elastic force vector is calculated according to the exact expression of curvature. The dynamic equations are derived by using the virtual work principle. The dynamic responses of a cantilever silica gel beam under gravity are calculated based on the present method and compared with those of the improved low-order beam element (ILOBE), high-order beam element (HOBE), and commercial finite element analysis software (ANSYS). Simulation results show that the proposed method can accurately describe the large deformation and large overall motion of the beam, and has better computational efficiency. Research in this paper provides an efficient dynamic model for the dynamics analysis of soft robot arms.
Effect of Fermentation of Arabica Coffee on Physicochemical Characteristics and Sensory Analysis
Authors: Vaz Carlos Johnantan Tolentino , Menezes Larissa Soares , Santana Ricardo Corrêa , Sentanin Michelle Andriati , Zotarelli Marta Fernanda , Guidini Carla Zanella 
Published: April 2022

Abstract
This work studied the physicochemical characteristics and final sensory quality of the Yellow Catuai IAC 62 fermented with Saccharomyces cerevisiae. An experimental design was done by a Composite Central Rotational Design (CCRD) to evaluate the fermentation time and temperature. The responses were: fermentative mass pH, fruit moisture (just as processes were conducted), sugars, organic acids content (HPLC), and sensory quality by Specialty Coffee Association (SCA) methodology on two different roasts levels (light-medium roast and medium roast). It was observed that coffees, even in extreme time and temperature, behave similarly related to final scores (in general from 81 to 85 in SCA score), all classified as specialty coffees. Fructose content varies only with fermentation time regardless of temperature, decreasing its levels as time increases, from 12g/L to near 5g/L. In contrast, sucrose content decreased while time and temperature advanced, from 4g/L to 2g/L in lowers time or temperature for sucrose, similar behavior for glucose from 7g/L to zero, however it shows some concentration increase on lowers time or temperature. Glycerol was formed depending on both time and temperature, and its content increased with time and temperature increase, showing optimum formation conditions between 24°C and 32°C and 35 to 45 hours. pH behaved just as described by literature, decreasing with time advance and more intensely as temperature increases, beginning from around 5 and getting to lower than 4 in extreme cases. Organic acids such as acetic, propionic, succinic, and lactic were measured at final fermentation and presented in different concentrations.
Differences in the health systems responsiveness in townships with different economic levels, and the role of general practitioners
Authors: Lu Jing , Hong Zhuang , Li Xiaohong , Sun Mei , Tang Qi , Chen Gang , Lu Jun 
Published: April 2022

Abstract
Background
The evaluation of health system responsiveness (HSR) can improve patient satisfaction, promote health equity and improve the quality of health services. There are many factors affecting HSR, but in view of China's national conditions, economic inequality and the promotion of general practitioners. Our research explored these two aspects.
Methods
Using a multi-stage stratified sampling method, 820 participants aged 18 and over were extracted in Kunshan City, China. Analysis of Variance was used in preliminary analysis. Linear regression was applied to explore the association of people in different economic towns with HSR score. The moderating effect of general practitioners was analyzed using Model 1 in Macro PROCESS program.
Results
Among the participants in the study, reported that mean (SD) HSR score was 34.7 ± 4.29. The association between the economic development level of towns and HSR was significant even after adjustment (β = -0.42; 95% Confidence Intervals, CI = -0.69-0.15; p = 0.002). In addition, hospital level (β = 0.75; 95% CI = 0.34–1.15; p < 0.001) and whether general practitioners are contracted (β = 2.61; 95% CI = 1.81–3.41; p < 0.001) were also important factors affecting the HSR score. Moderating effect analysis showed that general practitioners significantly moderated the effect of economic level on HSR score (P = 0.01).
Conclusions
Economic development level of towns was negatively correlated with the increase of HSR score. People in towns with good economic development level may have lower HSR scores. At the same time, general practitioners moderated the impact of economic development level on HSR score.
A single center study of two types of upper kidney preservation surgery for complete duplicate kidney in children
Authors: Zhang Xiansheng , Chu Han , Cao Yongsheng , Deng Qifei 
Published: April 2022

Abstract
Objective: To compare the efficacy, advantages and disadvantages of insertable ureteral reimplantation (UC group) and ureteral end-to-side anastomosis (UU group) in the treatment of duplicate kidney, and summarize the clinical experience in diagnosis and treatment. Methods: Retrospective analysis was performed on 20 cases of duplicate kidney in our hospital from April 2016 to June 2021, including 11 cases of insertable ureteral reimplantation and 9 cases of end-to-side ureteral anastomosis. There were 8 boys, 12 girls, 12 left and 8 right. There were 3 cases of urinary tract infection and 9 cases of urinary incontinence, all of the rest were found by B ultrasound in physical examination. The median age was 33.5 months. Preoperative and postoperative renal pelvis separation, ureteral dilation, operation time and drainage tube indwelling time were compared. Results: There was no significant difference in the degree of renal pelvis separation and ureter tortuosity before operation between the two groups (P > 0.05), and there was significant difference in the operative time between the two types of operation and in the duration of indwelling drainage tube (P < 0.05), there were significant differences in the degree of hydronephrosis and ureteral dilation before and after the two operations (P < 0.05), while there were no significant differences in the recovery of kidney and ureter after the two operations (P > 0.05). Conclusion: The two methods have their own advantages and disadvantages, both can be used as the choice of renal sparing therapy for complete duplicate kidney, it is necessary to choose the appropriate method according to the situation of children and the surgical experience of the doctors. Double J tube placement is helpful for finding and avioding to damage the duplicate ureter intraoperative, without increasing the difficulty of operation, and can prevent anastomosis or ureteral orifice stenosis.
Critical Contributions of Pre-S1 Shoulder and Proximal TRP Box in DAG-activated TRPC Channels by PIP2 Regulation
Authors: Mori Masayuki X. , Okada Ryo , Sakaguchi Reiko , Hase Hideharu , Imai Yuko , Polat Onur K. , Itoh Satoru G. , Okumura Hisashi , Mori Yasuo , Okamura Yasushi , Inoue Ryuji 
Published: April 2022

Abstract
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 or PIP2) regulates the activities of numerous membrane proteins, including diacylglycerol(DAG)-activated TRPC3/6/7 channels. Although PIP2 binding is known to support DAG-activated TRP channel activity, its binding site remains unknown. We screened for PIP2 binding sites within TRPC6 channels through extensive mutagenesis. Using voltage-sensitive phosphatase (DrVSP), we found that Arg437 and Lys442, located in the channel’s pre-S1 domain/shoulder, are crucial for interaction with PIP2. To gain structural insights, we conducted computer protein-ligand docking simulations with the pre-S1 domain/shoulder of TRPC6 channels. Further, the functional significance of PIP2 binding to the pre-S1 shoulder was assessed for receptor-operated channel functions, cross-reactivity to DAG activation, and the kinetic model simulation. These results revealed that basic residues in the pre-S1 domain/shoulder play a central role in the regulation of PIP2-dependent gating. In addition, neutralizing mutation of K771 in the proximal TRP box reversed the effect of PIP2 depletion from inhibiting to potentiating channel activity. A similar effect was seen in TRPV1 channels implies that TRPC6 possesses a common but robust polarity switch mediating the PIP2 binding effect. Overall, these mutagenesis studies reveal functional and structural insights for how basic residues and channel segments in TRP channels are controlled through phosphoinositide recognition.
Comparative study of Rivastigmine and Galantamine on the transgenic Drosophila model of Alzheimer’s Disease
Authors: Siddique Yasir Hasan , Naz Falaq , - Rahul , Varshney Himanshi 
Published: April 2022

Abstract

Alzheimer’s Disease (AD) is now becoming more prevalent in ageing populations worldwide. It is characterized as a progressive neurodegenerative disease most commonly associated with memory deficits and cognitive decline. The formation of amyloid plaques and neurofibrillary tangles are important pathological markers of AD. Cholinesterase inhibitors are widely used to decrease the hydrolysis of acetylcholine released from presynaptic neurons. In the present study we have studied the effect of rivastigmine and galantamine (commonly used cholinesterase inhibitors) on the transgenic Drosophila model of AD expressing human Aβ-42 in the neurons. The effect of similar doses of rivastigmine and galantamine (i.e. 0.1,1 and 10mM) was studied on the climbing ability, lifespan, oxidative stress markers, caspase 9 and 3, acetylcholinesterase activity and on the formation of Aβ-42 aggregates. The results suggest that the rivastigmine is more potent in reducing the oxidative stress and improving climbing ability of AD flies. Galantamine is a more potent inhibitor of acetylcholinesterase compared to rivastigmine. Galantamine prevents the formation of Aβ-42 aggregates more effectively compared to rivastigmine. The present study gives a comparative account of the rivastigmine and galantamine on the efficacy. The effect of the drugs taking the same doses have been studied on the climbing ability, life span, oxidative stress markers, apoptotic markers, acetylcholinesterase activity and on the formation of Aβ-42 aggregates. The study has been performed on the transgenic Drosophila model of AD expressing human Aβ-42 in the neurons.
Ceftazidime/avibactam-based versus polymyxin B-based therapeutic regimens for the treatment of carbapenem-resistant Klebsiella pneumoniae infection in critically ill patients: a retrospective cohort study
Authors: Zheng Guanhao , Cai Jiaqi , Zhang Liang , Chen Dayu , Wang Linyu , Qiu Yusi , Deng Han , Bai Hao , Bian Xiaolan , He Juan 
Published: April 2022

Abstract
Background: Considering the importance of ceftazidime/avibactam (CAZ/AVI) and polymyxin B (PMB) in treating carbapenem-resistant Klebsiella pneumoniae (CRKP) infection, it is essential to evaluate the efficacy and safety of these agents and provide appropriate medical advice to clinical specialists. Methods: We conducted a retrospective cohort study in two Chinese tertiary hospitals for critically ill patients with CRKP infection who received at least 24-hour CAZ/AVI-based or PMB-based treatment. A binary logistic model and a Cox proportional hazards regression model were constructed to analyze variables that could potentially affect 30-day microbiological eradication and all-cause mortality, respectively. Results: From January 2019 to December 2021, 164 eligible patients were divided into CAZ/AVI and PMB cohorts. A notably lower 30-day mortality rate (35.4% vs 69.5%, P < 0.001) and a higher 30-day microbiological eradication rate (80.5% vs 32.9%, P < 0.001) were observed for patients receiving CAZ/AVI-based treatment, compared with cases in PMB group. A longer antimicrobial treatment duration (> 7 days) could also significantly decrease the mortality rate and increase the microbiological eradication rate. Female patients had a higher survival rate than male. Age over 65 years, sepsis, Continuous Renal Replacement Therapy (CRRT) and organ transplantation were identified as negative factors on survival. In the subgroup analysis, we found that CAZ/AVI combined with tigecycline (Hazard ratio, 0.267; 95% Confidence Interval, 0.077-0.921; P = 0.037) or amikacin (Hazard ratio, 0.105; 95% Confidence Interval, 0.014-0.766; P = 0.026) could effectively lower mortality. According to safety evaluation results, potential hepatic enzymes elevation was associated with CAZ/AVI-based treatment, while renal impairment was probably related to PMB-based treatment. Conclusions: CAZ/AVI was more effective than PMB in treating CRKP infected patients. Tigecycline and amikacin were proved to be beneficial as concomitant agents in combination with CAZ/AVI. A treatment period lasting over 7 days was recommended. Although clinical safety of these two agents could be ensured because the severity level and incidence of adverse effects were relatively low, hepatoxicity of CAZ/AVI and nephrotoxicity of PMB should be monitored carefully. Further well-designed studies should be performed to verify our conclusion.
Establishment and validation of a pathologic upgrade prediction nomogram model for gastric low-grade intraepithelial neoplasia patients after the eradication of Helicobacter pylori
Authors: Ruan Yejiao , Lu Guangrong , Zhu Yuesheng , Ma Xianhui , Shen Yating , Shi Yuning , Zhang Xuchao , Zhu Zheng , Cai Zhenzhai , Xia Xuanping 
Published: April 2022

Abstract
Background: At present, there is no unified treatment for the evaluation and management of gastric low-grade intraepithelial neoplasia (LGIN) all over the world.Methods: Patients who were Helicobacter pylori eradicated, with low-grade gastric intraepithelial neoplasia were gathered.Several demographic and clinicopathological characteristics were described and analyzed retrospectively by LASSO regression analysis and multivariable logistic regression. Then the predictive nomogram was established. C-index, the area under the receiver operating characteristic curve (AUC) , calibration plot and decision curve analysis (DCA) were used to evaluate the accuracy and reliability of the model. Results: A total of 309 patients with LGIN were included, divided into training groups and validation groups randomly. LASSO regression analysis and multivariable logistic regression showed that six variables, gender, size, location, border line, number and erosion were independent risk factors for progression of gastric LGIN. The nomogram model displayed good discrimination with a C-index of 0.765 (95% confidence interval: 0.702–0.828). High C-index value of 0.768 could still be reached in the internal validation. The accuracy and reliability of the model was also verified by the AUC of 0.764 in the training group and 0.757 in the validation group. The calibration curve showed the model was in good agreement with the actual results as well. Decision curve analysis suggested that the predictive nomogram had clinical utility. Conclusions: A predictive nomogram model was successfully established and proved to identify high-risk groups with possible pathologic upgrade in patients with gastric LGIN. It suggested that after identifying high-risk patients, strengthening follow-up or endoscopic treatment may benefit in improving the detection rate or reducing the incidence of gastric cancer, which providing a reliable basis for the treatment of LGIN.