bluuebunny/crossref_metadata_2025_split huggingface dataset:
Attitudes and beliefs of older adults and caregivers towards deprescribing in French-speaking countries: a multicenter cross-sectional study
Authors: Roux Barbara , Rakheja Bianca , Sirois Caroline , Niquille Anne , Pétein Catherine , Ouellet Nicole , Spinewine Anne , Sibille François-Xavier , Laroche Marie-Laure 
Published: May 2022

Abstract
Purpose
Successful deprescribing requires understanding the attitudes of older adults and caregivers towards this process. This study aimed to capture these attitudes in four French-speaking countries, and to investigate associated factors.
Methods
A multicenter cross-sectional study was conducted by administrating the French version of the revised Patients’ Attitudes Towards Deprescribing (rPATD) questionnaire in Belgium, Canada, France and Switzerland. Community-dwelling or nursing home older adults ≥ 65 years taking ≥ 1 prescribed medications, and caregivers of older adults with similar characteristics were included. Multivariate logistic regressions were carried out to examine factors associated with willingness to deprescribe.
Results
A total of 367 older adults (79.3 ± 8.7 years, 63% community-dwelling, 54% ≥5 medications) and 255 unrelated caregivers (64.4 ± 12.6 years) of care recipients (83.4 ± 7.9 years, 52% community-dwelling, 69% ≥5 medications) answered the questionnaire. Among them, 87.5% older adults and 75.6% caregivers would be willing to stop medications if the physician said it was possible. Reluctance to stop a medication taken for a long time was expressed by 46% of both older adults and caregivers. A low score for the factor “concerns about stopping” [older adults: aOR: 0.21; 95%CI: 0.07–0.59], and a high score for the factor “involvement” [older adults: aOR: 2.66; 95%CI: 1.01–7.07; caregivers: aOR: 11.28; 95%CI: 1.48–85.91] were associated with willingness to deprescribe.
Conclusions
A significant proportion of older adults and caregivers of French speaking countries are open to deprescribing. Despite this apparent willingness, deprescribing conversations in clinical practice remains marginal, emphasizing the importance of optimizing the integration of existing tools such as rPATD.
MMP1 overexpression promotes cancer progression and associates with poor outcome in head and neck carcinoma
Authors: Zhang Wei , Huang Xinghong , Huang Rong , Zhu Hong , Ye Pu , Lin Xuyang , Zhang Shuangyue , Wu Meng , Jiang Feng 
Published: May 2022

Abstract
Purpose: Matrix metalloproteinase-1 (MMP1) has been reported to play key roles in a variety of cancers by degrading the extracellular matrix. However, its carcinogenic roles have not been shown yet in head and neck squamous cell carcinoma (HNSCC). This study aimed to elucidate its expression pattern, functional roles as well as clinical significance in HNSCC.Methods: The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and immunohistochemistry (IHC) were utilized to determine the MMP1 expression pattern and the associations between its expression and patients’ outcome in HNSCC. Mice tongue squamous cell carcinoma model induced by 4-nitroquinoline 1-oxide (4NQO), and siRNA mediated cellular assay in vitro were utilized to evaluate the oncogenic role of MMP1. The biological functions and cancer-related pathways involved in MMP1 related genes were found through bioinformatics analysis.Results: Both mRNA and protein abundance of MMP1 were highly increased in HNSCC as compared to its non-tumor counterparts. MMP1 overexpression positively correlated with advanced tumor size, cervical node metastasis and advanced pathological grade and lower patients’ survival. In the 4NQO-induced animal model, MMP1 expression increased along with the progression of the disease. In HNSCC cells, siRNA-mediated knockdown of MMP1 significantly inhibited cell proliferation, migration, and invasion, and activated apoptosis and epithelia-mesenchymal transition (EMT). GSEA, GO and KEGG analysis showed that MMP1 expression was significantly related to cancer-related pathways and cancer-related functions.Conclusion: Together, our results demonstrated MMP1 serves as a novel prognostic biomarker and putative oncogene in HNSCC.
Improvement of salt tolerance of wheat through co-inoculation with Azospirillum zeae and Serendipita indica
Authors: Zarea Mohammad Javad , Karimi Zohreh 
Published: May 2022

Abstract
Four experiments were carried out to investigate (1) the growth performances of S. indica in co-inoculation with Azospirillum zeae under supplemented tryptophan (Trp), (2) the effect of tryptophan on symbiosis performances of S. indica with wheat seedling experiment, (3) the effect of A. zeae and S. indica on wheat performances under salinity stress and (4) effect of A. zeae and S. indica on P5CS expression and proline accumulation. Trp inhibited S. indica growth, but introduced A. zeae as co-culture could mitigate the adverse effect of Trp on S. indica growth. Co-inoculation of S. indica with A. zeae gave better results in salt affected plants in terms of plant biomass, water content and photosynthetic pigments (chl-a, chl-b and carotenoids). While plants inoculated with S. indica accumulated higher concentration of proline in leaves, plants inoculated with Azospirillum showed a lower concentration of proline under both salinity and non-salinity stress. However, plants co-inoculated with A. zeae and S. indica showed lower accumulation of proline in leaves, but had higher concentration of N, chlorophyll content, water content and biomass. Salinity decreased shoot nitrogen contents, but the reductions were lower in inoculated plants than in the un-inoculated seedlings.
Leveraging location data of variable quality to reconstruct animal movements: application to a reintroduced island fox (Urocyon littoralis) population
Authors: Mummah Riley O. , Guglielmino Angela H. , Borremans Benny , Coonan Timothy J. , Prager K. C. , Lloyd-Smith James O. 
Published: May 2022

Abstract
Background: Despite significant advances in statistical approaches and data collection for analyzing wildlife movements over the last 50 years, there are limited analytical frameworks to be applied when spatial data are collected for purposes other than analyzing movement. Data collected for other purposes (e.g., sporadic captures or survival checks via telemetry) generally have lower temporal frequency or spatial precision than data collected to analyze fine-scale animal movement. The coarseness of the former renders them poorly suited for analysis using existing statistical tools. Methods: We propose a new way of estimating animal movement trajectories by integrating variable quality location data – including frequent but spatially coarse, irregularly-shaped polygon location data arising from VHF telemetry as well as less frequent, more spatially precise location data – using functional data analysis combined with a spatial resampling algorithm. We apply this method to analyze location data from the reintroduced Channel Island fox (Urocyon littoralis) subspecies population on Santa Rosa Island, California, which were collected from 2003-2012 for purposes other than movement analyses but provide an ideal case study to develop and test these novel methods. Results: By combining coarse-grained location knowledge, obtained through field notes and expert interpretation, with more precise location data, we reconstructed individual animal movement trajectories and demonstrated the utility of combining such data. Through the population ensemble of reconstructed trajectories, we learned that captive-born Channel Island foxes exhibited stronger seasonal movements than wild-born foxes, and most long-range movements occurred within the first two years of a fox’s time on the island. Conclusions: This methodology capitalizes on frequently overlooked but valuable spatial data, often found in field notes and expert knowledge, to reconstruct animal movements. Our approach has wide application to systems in which data of variable quality are collected for purposes other than studying movement and could have beneficial applications in species conservation, landscape ecology, disease management, and population monitoring.
B Cell Receptor Signaling Pathway Mutation as Prognosis Predictor of Immune Checkpoint Inhibitor for Lung Adenocarcinoma
Authors: Lin Anqi , Fang Jianbo , Cheng Quan , Liu Zaoqu , Luo Peng , Zhang Jian 
Published: May 2022

Abstract
Purpose: The advent of immune checkpoint inhibitors (ICIs) has generated a revolutionary breakthrough in the therapeutic treatment of many solid tumors, including lung adenocarcinoma (LUAD). However, the response rate of ICI therapy in patients with LUAD is low, so a clinical challenge has arisen in effectively using biomarkers to screen patients who can benefit from ICIs therapy. Methods: In this study, we divided patients according to BCR signaling pathway gene non-synonymous mutant or not, and established univariate and multivariate Cox regression models based on a LUAD cohort treated with ICI (Miao-LUAD), and examined the relationship between the mutation status of the BCR signaling pathway and the prognosis of immunotherapy. Then, combining the data from The Cancer Genome Atlas (TCGA) LUAD cohort, the Rizvi-LUAD, the Samstein-LUAD and the Zhujiang Hospital of Southern Medical University LUAD (Local-LUAD) cohort, the mutation panorama, immunogenicity, tumor microenvironment (TME) and pathway enrichment analysis between the BCR signaling pathway mutant group (BCR signaling MUT) and the BCR signaling pathway wild group (BCR signaling WT) were comprehensively compared. Results: It was found that, compared with the BCR signaling WT, the BCR signaling MUT had a significantly improved progression-free survival (PFS) and overall survival (OS), higher immunogenicity and immunoreactivity, and a pathway activation environment that was not conducive to the growth of tumor cells. Conclusion: These results revealed that the mutation state of the BCR signaling pathway has potential as a biomarker to predict the efficacy of ICIs.
Association between tamoxifen and tooth loss in women with breast cancer
Authors: Sensever Felipe Araujo , Jardim Luísa Comerlato , Ferrazzo Kívia Linhares , Skupien Jovito Adiel , Antoniazzi Raquel Pippi 
Published: May 2022

Abstract
Objectives
Investigate the effect of tamoxifen on the occurrence of tooth loss (TL) in breast cancer (BC) survivors.
Methods
A cross-sectional study was conducted with 140 BC survivors using tamoxifen therapy. Sociodemographic, medical and dental data were evaluated. TL was determined using the M component of the Decayed, Missing and Filled Teeth (DMFT) index. Logistic regression models were run to determine associations between the independent variables and outcome (TL).
Results
Mean TL was 12.96 (SD 8.88). Only three participants had lost no teeth and 10.7% were completely edentulous. Participants who used tamoxifen for more than 1 year had a higher mean missing teeth (13.99 vs. 10.45; P = 0.030). After the adjustments, the occurrence of more than 12 missing teeth was 2.75-fold higher among women who used tamoxifen for more than 1 year (95% CI: 1.06–7.12). Moreover, age over 65 years, referral for treatment by the public healthcare system, less use of dental services, xerostomia and a lower occurrence of dental caries remained associated with the loss of more than 12 teeth.
Conclusion
Longer use of tamoxifen was associated with greater tooth loss in breast cancer survivors.
Clinical relevance:
Based on estimates of the increase in cases of breast cancer, the prolonged use of tamoxifen for the treatment of this disease can exert an impact on oral health through the occurrence of tooth loss. These findings can contribute to the planning and implementation of oral health care and prevention strategies for such patients.
Influence mechanism of surface hardness of soil samples on plasma
Authors: Huang Yutao , Lin Jingjun , Lin Xiaomei 
Published: May 2022

Abstract
Laser-induced breakdown spectroscopy (LIBS) can be used to quantitatively analyze heavy metal elements in soil, but the matrix effects have always been an important problem affecting the accuracy of quantitative analysis. The influence mechanism of matrix effects on plasma needs to be further studied. In this paper, the main purpose is to analyze the influence mechanism of sample surface hardness on plasma. The trend of plasma temperature, the ratio of ion line to atomic line of Cr and Cu elements, and the calibration curve of Cr and Cu elements with the change of sample surface hardness are analyzed, respectively. The results show that the plasma temperature and the ratio of ion line to atomic line of Cr and Cu elements increase with the increase of sample surface hardness. Meanwhile, the root mean square error (RMSE) and average relative error (ARE) of Cr and Cu elements in the samples with a hardness of 98HA are lower than those of the samples with a hardness of 43HA and 70HA. For samples with a surface hardness of 98HA, the RMSE and ARE of Cr are 0.027wt% and 9.763%, and those of Cu are 0.047wt% and 15.862%, respectively. However, the stability of the training data decreases with the surface hardness increases. The relative standard deviations (RSD) of Cr and Cu elements are 19.383% and 34.383%, respectively, for samples with a surface hardness of 98HA. By studying the influence of sample surface hardness on plasma temperature and calibration curve, the influence mechanism of sample surface hardness on plasma was explored.
Detection and validation of hypoxia-related lncRNAs with associated ceRNA network involved in HCC prognosis, treatment responsiveness
Authors: Li Xiugai , Zheng Chang , Xue Xiaoxia , Wu Junying , Li Fei , Song Dan , Li Xuelian 
Published: May 2022

Abstract
Long non-coding RNAs can regulate hypoxia-induced tumor immune microenvironment remodeling and tumor progression. The present study aimed to build a risk model for predicting the prognosis of hepatocellular carcinoma (HCC) patients based on hypoxia-related lncRNAs and to explore its possible mechanisms. Based on the RNA-Seq and follow-up data downloaded from TCGA and GEO, we performed correlation analysis, differential analysis, and survival analysis to derive candidate hypoxia-related lncRNAs. In total, the 365 patients were used to randomly divide into the training and testing dataset at a ratio of 7:3. The Least Absolute Shrinkage and Selection Operator (Lasso)-Cox regression was applied to select and develop hypoxia-related lncRNAs signature (HRLS) in training datasets. We obtained a risk model consisting of 8 hypoxia-related lncRNAs which was systematically validated in the testing and GSE76427 dataset. HRLS was developed based on SNHG3, NRAV, AC073611.1, AL031985.3, AL049840.6, ZFPM2-AS1, AC074117.1, and MAFG-DT. Patients with low risk displayed a good prognosis, whereas those with high risk had a poor prognosis. Multivariate COX regression analysis also confirmed that the HRLS group was statistically significant after adjusting for clinical factors. Nomogram, time-dependent ROC curve, and decision curve analyses were performed to confirm the predictive ability. Furthermore, the high-risk patients had high-level infiltration of Macrophages and regulatory T cells. Next, AC073611.1 and AL031985.3 were found to be significantly decreased in Hep3B cells after hypoxia exposure (48 hours) in the GSE155505 dataset. Also, the expression of MAFG-DT was significantly upregulated in the sorafenib treatment responders of the GSE109211 dataset. The abnormal expression may be associated with hypoxia states. Finally, we constructed a novel ceRNA network and predicted their binding sites. We developed and validated a novel HRLS to accurately predict patient survival, assess immune infiltration, infer therapeutic benefits, and provide a new perspective for designing personalized therapies.
Comparative evaluation of robot-assisted and conventional urology surgeries in India
Authors: Mehmood Khalid , Singh Ranjana , Kumar Ashok , Mandal A K 
Published: May 2022

Abstract
There is dearth of evidence on patients’ perspective, their knowledge and understanding about robot-assisted surgeries, especially in Low- and Middle-Income countries such as India. The aim of this prospective study was to analyze inpatients’ and surgeon’s perspectives towards robot-assisted and conventional urological surgeries. A total of 136 patients (94 robot-assisted surgery cases and 42 conventional surgery cases) were enrolled in the study. A performa (with details such as sociodemographic details of patient, patient’s view point on surgery opted and doctor’s perspective on advising robot-assisted or conventional surgery) was used for data collection. Of 136 patients, 135 (99.3%) responded that their reason for opting the surgery (either robot-assisted or open laparoscopy) depended on the advice provided by the surgeon. 78 (83.3%) patients mentioned they opted for robot-assisted surgeries because of aesthetics (cosmetically better) in comparison to 07 (16.7%) patients who opted for conventional surgeries. Only 1.1% of surgeons reported robot-assisted surgeries to be technically easy. A few surgeons (1.1%) who operated with robot-assisted surgeries mentioned it to be time-consuming than 71.4% (30) surgeons in open/lap group. All the surgeons who treated patients with robot-assisted surgeries mentioned to have no of complications in comparison to 35.75% surgeons in open/lap group. The reason for choosing robot-assisted surgeries among both patients and surgeons were patient’s compatibility, cosmetically better, less-time consuming and less hospital stay. In addition, the surgeons also believed the robot-assisted surgeries to have fewer complications for their patients and therefore, recommended it.
Social-psychological and socio-structural correlates of negative attitudes toward asexuals’ parenting rights
Authors: Grigoropoulos iraklis 
Published: May 2022

Abstract
This study examined relations between political ideology, religiosity, individual characteristics, belief in a just world, and attitudes toward asexuals’ parenting rights aiming to gain a better understanding of the complex roots of sexual prejudice. An exploratory cross-sectional study was conducted between May 2 and July 30, 2021, via an online survey. Two hundred and four participants were recruited for this study. A between-subject, correlational design was employed. Bivariate correlation was generated to explore the associations between variables of interest. Next, a bootstrapping analysis for simple mediation models was conducted. Also, a multiple regression model was employed to predict attitudes towards asexuals’ parenting rights from the study measures. This study suggests that religious opposition to asexuals’ parenting rights is attributable, at least in part, to political ideology. In addition, gender is also a significant factor influencing the formation of attitudes toward asexuals’ parenting rights. In light of the current debates regarding the expansion of sexual and gender minority rights in several countries, this study presents a timely investigation into the underpinnings of opposition to asexuals’ parenting rights. Research data in this scarcely researched field may support the efforts of advocacy groups to promote social justice.
Novel EEG Quantification for Psychosis: Can We Analyze Vector Direction to Find A Pattern?
Authors: Aulia Afkar 
Published: May 2022

Abstract
Electroencephalography (EEG) had been a widely used diagnostic tool since its development in 1924. However, limitation exists, as it relies on visual recognition of spikes and quantification of brain waves. It might be possible to expand it's functionality by measuring the direction of electrical vectors, for example by comparing the value of coronal and sagittal voltage change during very short time period. This might be especially helpful for diseases with high inter-rater diagnosis bias, such as schizophrenia. We hoped that this method might expand research possibility and provided possible new method to reliably diagnose schizophrenia.
Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid
Authors: Kokubu Hiraku , Takahashi Toshifumi , Kabuto Miho , Kouzaki Hideaki , Fujimoto Noriki 
Published: May 2022

Abstract
The association between immunoregulatory cytokines, such as IL-10 or IL-35, and DPP-4 inhibitor-related bullous pemphigoid (BP) has not been evaluated. Sera were collected from 39 Japanese patients with BP (24 males and 15 females; 77.0 ± 11.1 years old) including 6 patients with non-DPP-4 inhibitor-related BP before treatment in our hospital. Ten healthy Japanese individuals (4 males and 6 females; 42.2 ± 9.37 years old) were enrolled as healthy controls. No significant difference was observed in serum IL-10 levels (BP patients: 7.63 ± 5.03 pg/ml; healthy individuals: 6.88 ± 0.52 pg/ml; DPP-4 inhibitor-related BP: 6.77 ± 0.24 pg/ml; non-DPP-4 inhibitor-related BP: 6.84 ± 0.20 pg/ml; BP vs healthy: P = 0.368; DPP-4 inhibitor-related BP vs non-DPP-4 inhibitor-related BP: P = 0.553), nor in serum IL-35 levels (BP patients: 2.62 ± 0.20 pg/ml; healthy individuals: 2.60 ± 0.17 pg/ml; DPP-4 inhibitor-related BP: 2.63 ± 0.17 pg/ml; non-DPP-4 inhibitor-related BP: 2.63 ± 0.21 pg/ml; BP vs healthy: P = 0.727; DPP-4 inhibitor-related BP vs non-DPP-4 inhibitor-related BP: P = 0.949). Bullous Pemphigoid Disease Area Index (BPDAI) before treatment was not related with serum IL-10 levels (r = 0.159; Fig. 1A), nor with serum IL-35 levels (r = 0.227; Fig. 1B). The number of serum eosinophils was significantly higher in patients with non-DPP-4 inhibitor-related BP (911.3 ± 948.8) than in patients with DPP-4 inhibitor-related BP (476.1 ± 234.0; P = 0.038). DPP-4 is also known as a CD26 molecule expressed on the surface of T lymphocytes. The mean rate of infiltrating CD26+ cells was significantly increased in 6 patients with DPP-4 inhibitor-related BP (32.9 ± 7.1) than in 6 patients with non-DPP-4 inhibitor-related BP (15.7 ± 4.4; P = 0.002; Fig. 2). It was reported that the co-engagement of CD3 and CD26 induces the preferential production of IL-10 from human CD4 + T cells, which might reflect the clinical characteristics of faint inflammatory bulla in DPP-4 inhibitor-related BP.