bluuebunny/crossref_metadata_2025_split huggingface dataset:
Desiccation increases the hatching of resting eggs of a freshwater calanoid copepod
Authors: Brazil Thamires , Caetano Ana Clara L , Vargas Anderson L , Bozelli Reinaldo L , Santangelo Jayme M 
Published: January 2022

Abstract
Many former permanent aquatic habitats now face occasional desiccation due to human activities. Zooplankton might recover from droughts after the hatching of resting eggs, but how desiccation duration affects the viability and hatching patterns of resting eggs is unclear. This study evaluated how desiccation of varying durations (0, 3, 6 and 9 months) affects the hatchling numbers of the freshwater calanoid Notodiaptomus iheringi inhabiting a permanent lake. Hatchlings were observed across all desiccation treatments, and hatchling abundance was higher in longer desiccation treatments. Resting eggs of N. iheringi survive desiccation for up to 9 months even though the tested population originated from sediments of a permanent lake. Desiccation may stimulate hatching by terminating dormancy and by weakening (wet) sediment adversity. Knowledge of the hatching patterns of resting eggs of varying taxa after desiccation can help to understand how zooplankton communities recover after droughts.
The tomato OST1–VOZ1 module regulates drought-mediated flowering
Authors: Chong Leelyn , Xu Rui , Huang Pengcheng , Guo Pengcheng , Zhu Mingku , Du Hai , Sun Xiaoli , Ku Lixia , Zhu Jian-Kang , Zhu Yingfang 
Published: January 2022

Abstract
Flowering is a critical agricultural trait that substantially affects tomato fruit yield. Although drought stress influences flowering time, the molecular mechanism underlying drought-regulated flowering in tomato remains elusive. In this study, we demonstrated that loss of function of tomato OPEN STOMATA 1 (SlOST1), a protein kinase essential for abscisic acid (ABA) signaling and abiotic stress responses, lowers the tolerance of tomato plants to drought stress. slost1 mutants also exhibited a late flowering phenotype under both normal and drought stress conditions. We also established that SlOST1 directly interacts with and phosphorylates the NAC (NAM, ATAF and CUC)-type transcription factor VASCULAR PLANT ONE-ZINC FINGER 1 (SlVOZ1), at residue serine 67, thereby enhancing its stability and nuclear translocation in an ABA-dependent manner. Moreover, we uncovered several SlVOZ1 binding motifs from DNA affinity purification sequencing analyses and revealed that SlVOZ1 can directly bind to the promoter of the major flowering-integrator gene SINGLE FLOWER TRUSS to promote tomato flowering transition in response to drought. Collectively, our data uncover the essential role of the SlOST1–SlVOZ1 module in regulating flowering in response to drought stress in tomato and offer insights into a novel strategy to balance drought stress response and flowering.
Heinz-resistant tomato cultivars exhibit a lignin-based resistance to field dodder (Cuscuta campestris) parasitism
Authors: Jhu Min-Yao , Farhi Moran , Wang Li , Philbrook Richard N , Belcher Michael S , Nakayama Hokuto , Zumstein Kristina S , Rowland Sarah D , Ron Mily , Shih Patrick M , Sinha Neelima R 
Published: January 2022

Abstract
Cuscuta species (dodders) are agriculturally destructive, parasitic angiosperms. These parasitic plants use haustoria as physiological bridges to extract nutrients and water from hosts. Cuscuta campestris has a broad host range and wide geographical distribution. While some wild tomato relatives are resistant, cultivated tomatoes are generally susceptible to C. campestris infestations. However, some specific Heinz tomato (Solanum lycopersicum) hybrid cultivars exhibit resistance to dodders in the field, but their defense mechanism was previously unknown. Here, we discovered that the stem cortex in these resistant lines responds with local lignification upon C. campestris attachment, preventing parasite entry into the host. Lignin Induction Factor 1 (LIF1, an AP2-like transcription factor), SlMYB55, and Cuscuta R-gene for Lignin-based Resistance 1, a CC-NBS-LRR (CuRLR1) are identified as factors that confer host resistance by regulating lignification. SlWRKY16 is upregulated upon C. campestris infestation and potentially negatively regulates LIF1 function. Intriguingly, CuRLR1 may play a role in signaling or function as an intracellular receptor for receiving Cuscuta signals or effectors, thereby regulating lignification-based resistance. In summary, these four regulators control the lignin-based resistance response in specific Heinz tomato cultivars, preventing C. campestris from parasitizing resistant tomatoes. This discovery provides a foundation for investigating multilayer resistance against Cuscuta species and has potential for application in other essential crops attacked by parasitic plants.
Expanding the plant genome editing toolbox with recently developed CRISPR–Cas systems
Authors: Wada Naoki , Osakabe Keishi , Osakabe Yuriko 
Published: January 2022

Abstract
Since its first appearance, CRISPR–Cas9 has been developed extensively as a programmable genome-editing tool, opening a new era in plant genome engineering. However, CRISPR–Cas9 still has some drawbacks, such as limitations of the protospacer-adjacent motif (PAM) sequence, target specificity, and the large size of the cas9 gene. To combat invading bacterial phages and plasmid DNAs, bacteria and archaea have diverse and unexplored CRISPR–Cas systems, which have the potential to be developed as a useful genome editing tools. Recently, discovery and characterization of additional CRISPR–Cas systems have been reported. Among them, several CRISPR–Cas systems have been applied successfully to plant and human genome editing. For example, several groups have achieved genome editing using CRISPR–Cas type I-D and type I-E systems, which had never been applied for genome editing previously. In addition to higher specificity and recognition of different PAM sequences, recently developed CRISPR–Cas systems often provide unique characteristics that differ from well-known Cas proteins such as Cas9 and Cas12a. For example, type I CRISPR–Cas10 induces small indels and bi-directional long-range deletions ranging up to 7.2 kb in tomatoes (Solanum lycopersicum L.). Type IV CRISPR–Cas13 targets RNA, not double-strand DNA, enabling highly specific knockdown of target genes. In this article, we review the development of CRISPR–Cas systems, focusing especially on their application to plant genome engineering. Recent CRISPR–Cas tools are helping expand our plant genome engineering toolbox.
Hydraulic vulnerability segmentation in compound-leaved trees: Evidence from an embolism visualization technique
Authors: Song Jia , Trueba Santiago , Yin Xiao-Han , Cao Kun-Fang , Brodribb Timothy J , Hao Guang-You 
Published: January 2022

AbstractThe hydraulic vulnerability segmentation (HVS) hypothesis implies the existence of differences in embolism resistance between plant organs along the xylem pathway and has been suggested as an adaptation allowing the differential preservation of more resource-rich tissues during drought stress. Compound leaves in trees are considered a low-cost means of increasing leaf area and may thus be expected to show evidence of strong HVS, given the tendency of compound-leaved tree species to shed their leaf units during drought. However, the existence and role of HVS in compound-leaved tree species during drought remain uncertain. We used an optical visualization technique to estimate embolism occurrence in stems, petioles, and leaflets of shoots in two compound-leaved tree species, Manchurian ash (Fraxinus mandshurica) and Manchurian walnut (Juglans mandshurica). We found higher (less negative) water potentials corresponding to 50% loss of conductivity (P50) in leaflets and petioles than in stems in both species. Overall, we observed a consistent pattern of stem &gt; petiole &gt; leaflet in terms of xylem resistance to embolism and hydraulic safety margins (i.e. the difference between mid-day water potential and P50). The coordinated variation in embolism vulnerability between organs suggests that during drought conditions, trees benefit from early embolism and subsequent shedding of more expendable organs such as leaflets and petioles, as this provides a degree of protection to the integrity of the hydraulic system of the more carbon costly stems. Our results highlight the importance of HVS as an adaptive mechanism of compound-leaved trees to withstand drought stress.
Variations in documentation of atrial fibrillation predicted by population and service level characteristics in primary health care in England
Authors: Routen Ash , Abner Sophia , Levene Louis S , Gillies Clare L L , Davies Melanie , Seidu Samuel , André Ng G , Khunti Kamlesh 
Published: January 2022

AbstractBackgroundIdentifying features associated with atrial fibrillation (AF) documentation could inform screening. This study used published data to describe differences in documented and estimated AF prevalence in general practices, and explored predictors of variations in AF prevalence.MethodsCross-sectional study of 7318 general practices in England. Descriptive and inferential statistics were undertaken. Multiple linear regression was used to model the difference between estimated AF and documented AF, adjusted for population, practice and practice performance variables.ResultsDocumented AF prevalence was lower than estimated (− 0.55% 95% confidence intervals, −1.89, 2.99). The proportion of variability accounted for in the final regression model was 0.25. Factors positively associated with AF documentation (increase in difference between estimated and documented), were patients 65–74 years, 75 years +, Black or South Asian ethnicity, diabetes mellitus and practices in East and Midlands of England. Eight variables (female patients, deprivation score, heart failure and peripheral artery disease, total patients per practice, full-time GPs and nurses; and location in South of England) were negatively associated with AF documentation (reduction in difference).ConclusionVariations in AF documentation were predicted by several practice and population characteristics. Screening could target these sources of variation to decrease variation and improve AF documentation.
Bone mineral density, osteopenia and osteoporosis among US adults with cancer
Authors: Huang J -F , Tan Q -C , Bai H , Wang J , Bergman M , Wu Z 
Published: January 2022

Abstract

Background
Bone mineral deficits are one of the most common complications in cancer survivors. However, there are no studies evaluating bone mineral density (BMD) and the prevalence of osteopenia and osteoporosis among patients with different types of cancers.

Aim
The objective was to assess BMD and evaluate the prevalence of osteopenia and osteoporosis among US adults with cancer.

Design
A cross-section propensity score matching study.

Methods
We extracted data from National Health and Nutrition Examination Survey database from 2005 to 2018. We compared BMD in participants with and without cancer which was further analyzed according to cancer type. We conducted logistic regression to evaluate adjusted odds ratios of osteopenia and osteoporosis and determine risk factors for their development.

Results
We found that BMD was significantly higher in participants without cancer than cancer patients. Furthermore, the median BMD of patients with breast cancer or skin cancer (including melanoma) was significantly lower than participants without cancer. People with breast, lung, genitourinary and skin cancers were more likely to incur osteopenia/osteoporosis than those without cancer.

Conclusions
BMD differs depending upon type in survivors. Individuals with a history of cancer have a poor understanding of osteoporosis and its risk factors. Understanding risk factors in patients with cancers identified in our study may be helpful for preventing osteoporosis and fractures and the development of screening guidelines.
Evaluating the Revised National Institutes of Health clinical trial definition impact on recruitment progress
Authors: Kane Eugene I , Daumit Gail L , Fain Kevin M , Scherer Roberta W , McGinty Emma Elizabeth 
Published: January 2022

Abstract

Background
The National Institutes of Health (NIH) announced a revised, expanded definition of ‘clinical trial’ in 2014 to improve trial identification and administrative compliance. Some stakeholders voiced concerns that the policy added administrative burden potentially slowing research progress.

Methods
This quasi-experimental study examined the difference-in-differences impact of the new NIH clinical trial definition policy on participant recruitment progress in grants funded by the National Institute of Mental Health (NIMH).

Results
One hundred thirty-two funded clinical trial grants were identified. While more grants were identified as clinical trials under the revised definition, the difference-in-differences in recruitment progress before and after the policy change was not statistically significant.

Conclusions
The revised NIH clinical trial definition had no clear effect on recruitment progress in newly identified NIMH-funded clinical trials as compared to traditionally identified clinical trials. Concerns that administrative delays and burden could impact study progress may be alleviated by these initial results.
A systematic review and meta-analysis of mycobacterial infections in patients with idiopathic inflammatory myopathies
Authors: Haldule Saloni , Chatterjee Moumita , Goswami Rudra Prosad , Vadsaria Innara , Gaur Prithvi , Kavadichanda Chengappa , Misra Durga Prasanna , Chinoy Hector , Agarwal Vikas , Aggarwal Rohit , Gupta Latika 
Published: January 2022

Abstract

Objectives
Infections including tuberculosis (TB) are a leading cause of morbidity and mortality in idiopathic inflammatory myopathies (IIM). We systematically reviewed the prevalence of mycobacterial infections in patients with IIM.

Methods
We screened PUBMED, EMBASE and SCOPUS databases and conference abstracts (2015–20) for original articles using Covidence. Pooled estimates of prevalence were calculated.

Results
Of 83 studies (28 cohort studies, two case control and 53 case reports), 19 were analysed. Of 14 043 IIM patients, DM (54.41%) was the most common subset among TB. Most studies were from Asia with high prevalence (5.86%, 2.33%–10.60%). Pooled prevalence of mycobacterial infections among IIM was 3.58% (95% CI: 2.17%, 5.85%, P &lt; 0.01). Disseminated and extrapulmonary forms (46.58%; 95% CI: 39.02%, 54.31%, P = 1.00) were as common as pulmonary TB (49.07%; 95% CI: 41.43%, 56.75%, P =0.99) both for I2=0. Muscle involvement, an otherwise rare site, was frequently seen in case reports (24.14%). M. tuberculosis (28.84%) was the most common pathogen followed by Mycobacterium avium complex (3.25%). Non-tuberculous mycobacteria were less common overall (6.25; 95% CI: 3.49%, 10.93%) I2=0, P =0.94. Subgroup analysis and meta-regression based on high vs low TB regions found prevalence 6.61% (2.96%, 11.33%) in high TB regions vs 2.05% (0.90%, 3.56%) in low TB regions. While death due to TB was occasionally reported (P =0.82), successful anti-tubercular treatment was common (13.95%).

Conclusion
TB is common in IIM, particularly in endemic regions though current data is largely heterogeneous. Extra-pulmonary forms and atypical sites including the muscle are frequent. Limited data suggests fair outcomes, although larger prospective studies may offer better understanding.
Pharmacological treatment in adult patients with CRPS-I: a systematic review and meta-analysis of randomized controlled trials
Authors: Fassio Angelo , Mantovani Alessandro , Gatti Davide , Rossini Maurizio , Viapiana Ombretta  , Gavioli Irene , Benini Camilla , Adami Giovanni 
Published: January 2022

Abstract

Objective
Several pharmacological treatments have been proposed for the treatment of complex regional pain syndrome type-I (CRPS-I) in adults, but data regarding the efficacy of various agents for this disease is scarce. We performed a systematic review and meta-analysis of randomized controlled trials (RCTs) to analyse the efficacy of the various pharmacological approaches in adults with CRPS-I.

Methods
We systematically searched PubMed, Scopus, and Web of Science databases from the inception date to 30 June 2021 to identify placebo-controlled or active-controlled RCTs using bisphosphonates, ketamine, CSs, anti-epileptics, NSAIDs/COXIBs, opiates, antidepressants, scavengers/magnesium sulphate or IVIGs for the treatment of CRPS-I. The primary outcomes included changes in the visual analogue scale (VAS) or numeric rating scale (NRS) for pain before and after treatment.

Results
We included 20 placebo-controlled or active-controlled RCTs (including a total of 818 adults with CRPS-I) that used bisphosphonates (n = 7), ketamine (n = 2), CSs (n = 2), anti-epileptics (n = 1), NSAIDs/selective inhibitors of cyclooxygenase-2 (COXIBs) (n = 2), scavengers/magnesium sulphate (n = 5), or IVIGs (n = 1) to treat CRPS-I during a median follow-up of 26 weeks. Treatment with bisphosphonates showed a significant reduction in the values of the VAS/NRS pain scale compared with placebo or reference therapy (random effects weighted mean difference [WMD]: −23.8, 95% CI: −28.0 to −19.6; I2 = 36.4%). Treatment with ketamine also documented a reduction in the values of the VAS/NRS for pain (random effects WMD: −8.27, 95% CI: −12.9 to −3.70; I2 = 0%). Treatment with other agents did not reduce the values of the VAS/NRS assessments of pain.

Conclusion
This systematic review and meta-analysis supports the recommendation of parenteral bisphosphonates as the first-line agent in the treatment of CRPS-I.

Trial registration
Open Science Framework registries, https://osf.io/et9gu/, osf.io/et9gu.
Ultrasound findings of calcium pyrophosphate deposition disease at metacarpophalangeal joints
Authors: Cipolletta Edoardo , Di Matteo Andrea , Smerilli Gianluca , Di Carlo Marco , Di Battista Jacopo , Abhishek Abhishek , Grassi Walter , Filippucci Emilio 
Published: January 2022

Abstract

Objective
To explore the spectrum of articular and peri-articular ultrasound (US) findings at metacarpophalangeal (MCP) joints in calcium pyrophosphate (CPP) deposition disease (CPPD).

Methods
Consecutive CPPD patients (chronic CPP crystal inflammatory arthritis or OA with CPPD), and age- and sex-matched controls with RA were prospectively enrolled. Patients underwent bilateral US examination of MCP joints. CPP deposits, synovial inflammation, osteophytes, cartilage damage and bone erosions were recorded.

Results
Sixty CPPD patients (33, 55.0% with OA with CPPD and 27, 45.0% with chronic CPP crystal inflammatory arthritis) and 40 RA patients were enrolled. CPP deposits were detected in 24 (40.0%) CPPD patients and in 3 (7.5%) RA patients (P &lt;0.01). In CPPD patients, different types of CPP deposits were identified at MCP joints: 17 (28.3%) patients had dorsal capsuloligamentous deposits, 14 (23.3%) intra-cartilaginous deposits, 13 (21.7%) lateral capsuloligamentous deposits, 12 (20.0%) intra-articular deposits, eight (13.3%) double contour sign and five (8.3%) flexor digitorum tendons’ deposits. CPPD patients with chronic CPP crystal inflammatory arthritis showed more US findings indicating synovial inflammation and CPP deposits than those with OA with CPPD. Conversely, a higher prevalence of US features indicating structural damage was noted in this latter phenotype. CPP deposits and bone erosions were the US findings with the highest value for diagnosing chronic CPP crystal inflammatory arthritis and RA, respectively.

Conclusion
This study provides pictorial evidence of the broad spectrum of US findings indicating CPP deposits at MCP joints in CPPD. Furthermore, we reported different US patterns in different CPPD phenotypes.
Impaired Membrane Lipid Homeostasis in Schizophrenia
Authors: Li Minghui , Gao Yan , Wang Dandan , Hu Xiaowen , Jiang Jie , Qing Ying , Yang Xuhan , Cui Gaoping , Wang Pengkun , Zhang Juan , Sun Liya , Wan Chunling 
Published: January 2022

Abstract

Background and Hypothesis
Multiple lines of clinical, biochemical, and genetic evidence suggest that disturbances of membrane lipids and their metabolism are probably involved in the etiology of schizophrenia (SCZ). Lipids in the membrane are essential to neural development and brain function, however, their role in SCZ remains largely unexplored.

Study Design
Here we investigated the lipidome of the erythrocyte membrane of 80 patients with SCZ and 40 healthy controls using ultra-performance liquid chromatography-mass spectrometry. Based on the membrane lipids profiling, we explored the potential mechanism of membrane phospholipids metabolism.

Study Results
By comparing 812 quantified lipids, we found that in SCZ, membrane phosphatidylcholines and phosphatidylethanolamines, especially the plasmalogen, were significantly decreased. In addition, the total polyunsaturated fatty acids (PUFAs) in the membrane of SCZ were significantly reduced, resulting in a decrease in membrane fluidity. The accumulation of membrane oxidized lipids and the level of peripheral lipid peroxides increased, suggesting an elevated level of oxidative stress in SCZ. Further study of membrane-phospholipid-remodeling genes showed that activation of PLA2s and LPCATs expression in patients, supporting the imbalance of unsaturated and saturated fatty acyl remodeling in phospholipids of SCZ patients.

Conclusions
Our results suggest that the mechanism of impaired membrane lipid homeostasis is related to the activated phospholipid remodeling caused by excessive oxidative stress in SCZ. Disordered membrane lipids found in this study may reflect the membrane dysfunction in the central nervous system and impact neurotransmitter transmission in patients with SCZ, providing new evidence for the membrane lipids hypothesis of SCZ.
Discursive multivocality: how the proliferation of economic language can undermine the political influence of economists
Authors: Rilinger Georg 
Published: January 2022

Abstract
This article shows how the proliferation of economic language can undermine the political authority of economists. The argument emerges from a comparative case study of two early experiments with electricity market design. Relying on archival materials and 30 in-depth interviews, I examine why political actors ignored the advice of economists in California, while they deferred to the experts in the Pennsylvania, Jersey, Maryland (PJM) region. The debates were framed in economic language, but stakeholders interpreted central concepts differently without recognizing the resulting ambiguities. This ‘discursive multivocality’ undermined economists’ authority as experts. It challenged economists’ monopoly on the interpretation of economic concepts and undercut rhetorical strategies to reassert the superiority of their understanding. At the PJM Interconnect, the experts overcame this problem by switching to a different conceptual apparatus. Ironically, economists could establish their authoritative understanding of economics by appealing to a shared understanding of engineering problems.