bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microvascular alterations of peripapillary choriocapillaris in young adult high myopia detected by optical coherence tomography angiography
Authors: Lei Jie , Fan yuanyuan , Bian Cunxin , Yuan Songtao , Ye Yurong , Huang Kun , Chen Qiang , Hu Zizhong , Liu Qinghuai , Yang Bin , Xie Ping 
Published: November 2022

Abstract
Objectives.
To characterize the microstructural alterations of peripapillary choriocapillaris in young adult high myopes using optical coherence tomography angiography (OCTA) and to explore factors involved in the alterations.
Methods.
This cross-sectional control study included 205 eyes (95 with high myopia and 110 with mild-moderate myopia). The choroidal vascular network was imaged using OCTA, and the images underwent manual adjustments to determine the peripapillary atrophy (PPA)-β zone and microvascular dropout (MvD). The area of MvD and PPA-β zone, spherical equivalent (SE), and axial length (AL) were collected and compared across groups.
Results.
The MvD was identified in 195 eyes (95.1%). Highly myopic eyes exhibited significantly greater area of PPA-β zone (1.221 ± 0.073 vs. 0.562 ± 0.383 mm2, P = 0.001) and MvD (0.248 ± 0.191 vs. 0.089 ± 0.082 mm2, P < 0.001) compared with mildly-moderately myopic eyes, and lower average density in the choriocapillaris. Linear regression analysis showed that MvD area correlated age, SE, AL, and PPA-β area (all P < 0.05).
Conclusions.
MvDs represent choroidal microvascular alterations in young adult high myopes, which were correlated with age, SE, AL, and PPA-β zone. In this disorder, OCTA matters for characterizing the underlying pathophysiological adaptations.
Epi-Clock: A sensitive platform to help understand pathogenic disease outbreaks and facilitate the response to future outbreaks of concern.
Authors: Ji Cong , Shao Junbin Jack 
Published: November 2022

Abstract
Background
The lack of virus fossilization precludes any references or ancestors for inferring evolutionary processes, and viruses have no cell structure, metabolism, or space to reproduce outside host cells. Most mutations yielding high pathogenicity go extinct from the population, but adaptive mutations could be epidemically transmitted and fixed in the population. Therefore, determining how viruses originated, how they diverged and how an infectious disease was transmitted are serious challenges.
Methods
To predict potential epidemic outbreaks, we tested our strategy, Epi-Clock, which applies the ZHU algorithm on different SARS-CoV-2 datasets before outbreaks to search for real significant mutational accumulation patterns correlated with the outbreak events. We imagine that specific amino acid substitutions would be triggers for outbreaks.
Results
Surprisingly, some inter-species genetic distances of Coronaviridae were shorter than the intra-species distances, which may represent the intermediate states of different species or subspecies in the evolutionary history of Coronaviridae. The insertions and deletions of whole genome sequences between different hosts were separately associated with new functions or turning points, clearly indicating their important roles in the host transmission and shifts of Coronaviridae. Furthermore, we believe that non-nucleosomal DNA may play dominant roles in the divergence of different lineages of SARS-CoV-2 in different regions of the world because of the lack of nucleosome protection. We suggest that strong selective variation among different lineages of SARS-CoV-2 is required to produce strong codon usage bias. Interestingly, we found that an increasing number of other types of substitutions, such as those resulting from the hitchhiking effect, have accumulated, especially in the pre-breakout phase, even though some previous substitutions were replaced by other dominant genotypes. From most validations, we could accurately predict the potential pre-phase of outbreaks with a median interval of 5 days before. Using our pipeline, users may review updated information on the website https://bioinfo.liferiver.com.cn with easy registration.
Conclusions
Here, we propose Epi-Clock, a sensitive platform to help understand pathogenic disease outbreaks and facilitate the response to future outbreaks, similar to a clock that can signal the need to assist individuals at focal locations by using diagnostics, isolation control, vaccines or therapy at any time.
Changes in biomarkers of redox status in serum and saliva in dogs with hypothyroidism
Authors: Arostegui Luis G. González , Prieto Alberto Muñoz , Marín Luis Pardo , López Gregorio García , Tvarijonaviciute Asta , Cerón José Joaquín , Rubio Camila Peres 
Published: November 2022

Abstract

Background: Hypothyroidism is the most common endocrine disorder diagnosed in dogs, leading to deleterious effects on dog’s life quality. This study aims to evaluate changes in the redox status in canine hypothyroidism. For this purpose, a comprehensive panel of antioxidants and oxidants was measured in serum and saliva of 23 dogs with hypothyroidism, 20 dogs with non-thyroidal illness and 16 healthy dogs. Among the antioxidants, cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma or saliva (FRAP or FRAS, respectively), Trolox equivalent antioxidant capacity (TEAC),thiol, paraoxonase type 1 (PON-1) and glutathione peroxidase (GPx) were determined. The oxidant biomarkers included were total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS).
Results: Results showed a significantly higher TEAC, PON-1, GPx, TOS, POX-Act and d-ROMs, and a significantly lower of AOPP in serum of dogs with hypothyroidism, except when AOPP was corrected by serum albumin concentration. Meanwhile, a significantly lower FRAS and AOPP was observed in saliva of dogs with hypothyroidism. Once salivary concentrations were corrected based on their total protein concentrations, the only analyte showing significant changes was TBARS that was significantly higher in dogs with hypothyroidism
Conclusions: Our results show thatdogs with hypothyroidism present alterations in the redox status in both serum and saliva. This study should be considered a preliminary study and further research addressing these changes should be made using larger populations.
Gastroenteroanastomosis with a linear or circular stapler? Is there really a preference for laparoscopic gastric bypass?
Authors: Netto Matheus , Tinoco Augusto , FonteBôa Igor , Duval-Araújo Ivana 
Published: November 2022

AbstractBACKGROUND: Laparoscopic Roux-en-Y gastric bypass (LRYGB) is an effective and well-accepted procedure for the treatment of morbid obesity but has complications such as stenosis of the gastroenteroanastomosis (GE), GE leak, surgical site infection, and stapling malfunction. Some aspects related to the surgical technique have been described in the literature as the cause of these complications, especially stenosis of the gastrojejunal anastomosis. This study evaluated the efficiency of weight loss and the incidence of short- and mid-term postoperative complications in patients undergoing LRYGB in which anastomosis was performed using a linear stapler (LSA) or a circular stapler (CSA). METHODS: Prospective observational study conducted between April 2016 and March 2019. The data were extracted from a database that includes patients undergoing LRYGB performed by the same surgeon and at the same institution. Data were collected on gastroenteroanastomosis technique, postoperative complications, and excess body weight loss at 30, 180, and 360 days. RESULTS: Data from 457 patients were analyzed, of which 216 patients were in the LSA group and 241, in the CSA group. There was no significant difference between the groups regarding initial BMI, age, gender, and comorbidities. Operative time, hospital stay, and excess body weight loss during the 12-month follow-up period were also similar in the two groups. There were four cases (1.7%) of GE stenosis in the CSA group and only one case (0.5%) in the LSA group, which were treated with one successful endoscopic dilatation. Stapler malfunction occurred in both groups: CSA (0.4%) and LSA (0.5%), and was repaired with intraoperative manual suture, and a GE leak developed (0.4%) only in the CSA group, which was successfully treated conservatively. Surgical site infection was found in five patients in the CSA group (2.1%) and two patients in the LSA group (0.9%). No statistical difference was found between the two groups in any of the variables analyzed (p>0.05). CONCLUSIONS: Both stapling techniques resulted in a similar loss of excess body weight during the follow-up period. Although the LSA group had fewer total complications, these were not statistically significant, which substantiates the fact that both techniques are safe and feasible, provided they are performed by a surgeon with a long learning curve in laparoscopic bariatric surgery.
Assessment of hydrogeochemistry of shallow aquifer using corrosion indices and geospatial techniques in the regions of Brahmaputra River Basin, India
Authors: Sharma Gulshan Kumar , Jena Roomesh Kumar , Moharana Pravash Chandra , Ray Prasenjit , Ali Shakir , Mourya Krishna Kumar , Das Bachaspati 
Published: November 2022

Abstract

Present study was conducted on groundwater hydrogeochemistry in the Majuli River Island of the Brahmaputra River, India. Forty three groundwater samples were collected from shallow aquifers from different sites in the Majuli river basin, and analysed for various physico-chemical water quality parameters. Study showed that nearly 27.0 and 79.10, 88.43 and 74.41% of groundwater samples had higher nitrate, total hardness, iron and manganese content, respectively than the acceptable limits of the Bureau of Indian Standards (BIS) for drinking water. The water quality index revealed that 72.0% samples were not suitable for human drinking purpose. For analysing the corrosiveness and scaling potential of groundwater, indices namely, Langelier saturation index (LSI), Ryznar stability (RSI), Puckorius scaling (PSI), Larson skold indexes (LaI) and Chloride sulphate mass ratio (CSMR) were computed. The Ryznar index value of 70.0% collected groundwater samples were found to be more than 7.0 depicting significant corrosion towards the groundwater. Similarly, 90.69% samples had the PSI value > 6.5, representing tendency for corrosion. The CSMR value of 95.34% samples were > 0.5 in the study area. These indices also revealed that majority of groundwater samples of the Majuli River Island were corrosive in nature due to its non-alkaline characteristic and high iron content in the groundwater. The groundwater of the Majuli River Island may cause detrimental impacts on the domestic and industrial water quality and water distribution systems.
Homology-Directed Repair of an MYBPC3 gene mutation in a rat model of hypertrophic cardiomyopathy
Authors: Nie Jiali , Han Yu , Jin Zhiyuan , Hang Weijian , Shu Hongyang , Wen Zheng , Ni Li , Wang Dao Wen 
Published: November 2022

Abstract
Variants in myosin-binding protein C3 (MYBPC3) gene are a main cause of hypertrophic cardiomyopathy (HCM), accounting for 30–40% of the total number of HCM cases. Gene editing represents a potential permanent cure for HCM. The aim of this study was to investigate whether genome editing of MYBPC3 using the CRISPR/Cas9 system in vivo could rescue the phenotype of rats with HCM. We generated a rat model of HCM (“1098hom”) that carried an Mybpc3 premature termination codon mutation (p.W1098x) discovered in a human HCM pedigree. On postnatal day 3, the CRISPR/Cas9 system was introduced into rat pups by a single dose of AAV9 particles to correct the variant using homology-directed repair (HDR). Analysis was performed 6 months after AAV9 injection. The 1098hom rats didn’t express MYBPC3 protein and developed an HCM phenotype with increased ventricular wall thickness and diminished cardiac function. Importantly, CRISPR HDR genome editing corrected 3.56% of total mutations, restored MYBPC3 protein expression by 2.12%, and normalized the HCM phenotype of 1098hom rats. Our work demonstrates that the HDR strategy is a promising approach for treating HCM associated with MYBPC3 mutation, and that CRISPR technology has great potential for treating hereditary heart disease.
Minimally invasive surgery for pleuroperitoneal communication complicating peritoneal dialysis
Authors: Wang Luming , Liu Jiacong , Wang Yaomin , Zhu Linhai , Hu Jian 
Published: November 2022

Abstract
Objective
Pleuroperitoneal communication (PPC) is an uncommon but serious complication of continuous ambulatory peritoneal dialysis (CAPD). At present, there are many kinds of treatment options, with different effects. We describe our single-institutional experiences in the minimally invasive surgery of pleuroperitoneal communication complicating continuous ambulatory peritoneal dialysis in detail.
Methods
Our study consecutively enrolled twelve pleuroperitoneal communication patients complicating CAPD. All patients underwent direct closure of the defective diaphragm and mechanical rub pleurodesis under video-assisted thoracoscopy. What’s more, pseudomonas aeruginosa injection was infused into the thoracic cavity postoperatively to further promote pleural adhesion, which was the innovation of our study. The follow-up date was not ended until termination of the study.
Results
After 1.0-8.3 months (mean: 3.6 months) of CAPD, all twelve patients occurred hydrothorax in the right side. And all these patients received surgery 7-179 days (mean: 38.3 days) after onset. The mean operation time was 63.4 min (range, 27–150 min). Bleblike lesions situated on the diaphragm were discovered in all patients and three patients also had obvious hole on the surface of diaphragm. Pseudomonas aeruginosa injection was infused into the thoracic cavity postoperatively, and three cases occurred fever with remission after 2–3 days of symptomatic treatment. The time from surgery to restarting CAPD ranged from 14 to 47 days, with an average of 22.3 days. There was no recurrence of hydrothorax and transformation to hemodialysis during the follow-up period (mean: 9.0 months).
Conclusions
Video-assisted thoracoscopic direct closure of the defective diaphragm and mechanical rub pleurodesis plus chemical pleurodesis using pseudomonas aeruginosa injection postoperatively is a safe and effective option for the treatment of pleuroperitoneal communication complicating continuous ambulatory peritoneal dialysis with 100% success rate.
Global research trends on immunotherapy in cancer: a visualization analysis
Authors: Wu Weijia , Zhao Lei 
Published: November 2022

Abstract
Background
Cancer immunotherapy has been gradually introduced and has undergone noteworthy developments in recent years. The number of scientific publications has been expanding, and the progression in this field has been rapidly evolving with time.
Objective
This study aimed to use bibliometric analysis of the published literature on immunotherapy in cancer to illustrate the research trends over the past 20 years and to summarize the hotspots for future studies.
Methods
A literature search for medical publications on immunotherapy in cancer from 2000 to 2021 was conducted in the Web of Science Core Collection on March 1, 2022. Visualization analysis was performed using VOSviewer software (version 1.6.16).
Results
From 2000 to 2021, a total of 18,778 publications were retrieved. Annual publication output grew rapidly from 366 in 2000 to 3,194 in 2021, with a clear increase in publications in 2017. The USA issued the largest number of publications (n = 6,739, 35.89%), with the University of Texas System making the largest contribution (n = 802, 4.27%). A total of 976 meaningful topics were identified and further classified into 4 different clusters. The most common research topics included ‘expression’, ‘chemotherapy’, ‘dendritic cells’, ‘pembrolizumab’ and ‘open-label’. Highly identified cancer types included hepatocellular, bladder, breast and lung cancer. A shift in popularity from mechanism research to clinical trials was observed, indicating that clinical application would be the center of attention in the future.
Conclusions
Attention has been given to the field of cancer immunotherapy, and this trend will continue in the future. This study provides an unbiased visualization analysis on this topic in a scale-efficient manner for further research.
Effects of nonlinear hyper-viscoelasticity and matrix/fiber debonding on mechanical properties of short carbon fiber/SBR composites under cyclic uniaxial loads using an RVE-based multiscale finite element model
Authors: Andideh Mohammad , Ghoreihy Mir Hamid Reza , Soltani Sedigheh , Abbassi-Sourki Foroud 
Published: November 2022

AbstractThis research work is devoted to the development of an RVE-based finite element analysis of short carbon fiber (SCF) reinforced rubber composites under uniaxial tensile loads by a novel approach. A micro model was developed with periodic geometry and random distribution of the short fiber in it. Three different zones including rubber matrix, SCF as the inclusion phase, and a thin layer as the interphase were considered. A nonlinear hyper-viscoelastic model was selected for the matrix in conjunction with linear viscoelastic and elastic models for the interphase and reinforcing parts, respectively. The analyses were carried out at two loading-unloading rates of 10 mm/min and 100 mm/min subjected to incrementally-increased cyclic loads. Two interface conditions were taken into account. In the first case, a perfect bonding was assumed between matrix and SCF while in the second, partial debonding between fiber and polymer was considered. The latter was modeled via XFEM with a crack initial criterion. An integral averaging technique was employed to predict the stress and strain at the macro-scale. Comparison of the predicted results with experimentally measured data revealed that the adopted methodology and modeling techniques are quite able to predict the stress and strain and thus confirmed the accuracy and correctness of the multiscale approach and selected material models.
Effects of psychological intervention under ERAS on recovery,HAMA,HAMD and pulmonary function in patients with lung cancer surgery
Authors: Li Ting , Wang Jiyun , Hou Bofeng , Li Lan , Zhang Xiaohui , Zou Cuijie , Wang Chunyan 
Published: November 2022

Abstract
[Abstract] Backgroud: The improvement effect of ERAS combined with psychological intervention on lung function and psychological status of lung cancer surgery subjects was analyzed. Methods: The clinical data of 90 patients with lung cancer surgery were collected and randomly divided into two groups.The group receiving enhanced recovery after surgery(ERAS)combined with psychological intervention was the ERAS group(n=45),and the group receiving routine intervention was the single group(n=45);The baseline data,postoperative recovery,psychological improvement,pain improvement at different time points,pulmonary function level,quality of life,complications and satisfaction were analyzed. Results: The results showed that the postoperative recovery,psychological improvement,pain improvement at different time points,pulmonary function level,quality of life,complications and satisfaction in the ERAS group were significantly better than those in the conventional group(P<0.05). Conclusion: It shows that the clinical efficacy of ERAS combined with psychological intervention is better than that of conventional intervention,which can significantly improve the pulmonary function level and psychological problems of patients,promote recovery,and has certain safety and is more easily accepted by patients.
An Effective Method to Prevent Sports Injury and Analysis of Related High Risk Factors in Public Health
Authors: Dai Danfeng , Zhang Miaolin , Shen Baojie , Ruan Di 
Published: November 2022

Abstract
Methods
A random sample of 140 college football players is surveyed using injured players (n = 110) and uninjured players (n = 30) to identify significant variables between injured and uninjured players.
Results
In this paper, epidemiological and sociological methods are used to investigate the characteristics of sports injuries among college football players, and the risk factors of sports injuries were studied in depth, so as to obtain the characteristics and characteristics of sports injuries and provide a basis for the prevention of sports injuries in the future.
Conclusion
Logistic regression model analysis results show that sports quality, sports level, training level, attendance times and time, different venues and other factors were the risk factors of college students football injury.
Environmental concerns, physical and mental health among Palestinians: The mediating roles of optimism, pessimism and meaning in life
Authors: Bdier Dana , Veronese Guido , Mahamid Fayez , Kittaneh Hala 
Published: November 2022

Abstract
We investigated the association between environmental concerns, health and mental health among Palestinians and whether optimism, pessimism and meaning in life mediate the association between these variables. The sample of our study consisted or 504 participants were selected using online tools. The findings revealed that pessimism negatively correlated with optimism, mental health, physical health, and meaning in life, while optimism positively correlated with mental health, physical health, and meaning in life and negatively correlated with environmental concerns. Moreover, meaning in life correlated negatively with environmental concerns. Structural equation modelling (SEM) analysis showed that optimism, pessimism, and meaning in life mediate the correlation between environmental concerns, health and mental health with a good fit for our model. In the case of Palestinian territories, environmental concerns and issues are driven mainly by politics affecting people's health and mental well-being. Therefore, it is recommended to conduct similar studies to explore protective factors against environmental concerns in the Palestinian context, which will help develop appropriate interventions to enhance mental health in a society characterized by high stressors and prolonged trauma