bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short-term impact of COVID-19 infection on right ventricular functions: single center observational study
Authors: Rifaie Osama , Reda Ahmed , Hatata Ahmed , Gamal Amr , Abdelmonaem Mostafa 
Published: February 2022

Abstract
Background
COVID-19 pandemic is associated with high morbidity and mortality. Cardiovascular insult is a leading cause of in-hospital mortality in COVID-19 patients, especially right ventricular (RV) dysfunction and massive pulmonary embolism. This study aims to assess short-term impact of COVID 19 infection on (RV) functions among hospitalized patients with moderate or severe illness using bed side trans-thoracic echocardiogram. This study was conducted in 3 isolation hospitals in Cairo, spanning over 3 months during the expected pandemic peak in Egypt in 2020. The study recruited 100 consecutive patients with moderate or severe COVID-19 infection. Four patients refused to participate in the study. Patients with pre-existing structural heart diseases were excluded. All patients underwent full history taking and clinical examination. Bed side echocardiography was done emphasizing on (RV), and (RA) dimensions, (LV) functions and pulmonary artery systolic pressure (PSAS). Cardiac biomarkers were withdrawn and CT angiography was ordered when clinically warranted.

Results
The mean age of the studied cohort was 59.5 ± 8.6 years with males comprising 71.9% of the studied group. (RV) and (RA) dilatation was noted in 8 cases (8.3%). (LV) dysfunction was noted in 11 cases (11.4%). (PASP) showed a statistically significant negative correlation with (LV) function. However, (PSAP) was positively correlated to (RA) and (RV) dimensions, tricuspid regurgitation (TR) jet severity, previous COVID infection and elevated cardiac biomarkers. Mortality was noted in 3 cases (3.1%), all had LV dysfunction with elevated troponin level. Six patients (6.2%) had combined (LV) and (RV) dysfunction.

Conclusions
COVID-19 illness had a negative impact on (RV) and (LV) functions, that could be assessed accurately by trans-thoracic 2 D echocardiogram. The degree of ventricular dysfunction correlated with the rise in cardiac biomarkers as well as the degree of (PASP).
Anxiety and depression among vaccinated anesthesia and intensive care doctors during COVID-19 pandemic in United Arab Emirates: a cross-sectional study
Authors: Rizk Dalia Nagui , Abo Ghanima Mohamed 
Published: February 2022

Abstract
Background
The coronavirus disease 2019 (COVID-19) pandemic had an impact on frontline healthcare workers’ (HCW) mental health as they experienced depression, anxiety, and sleep disturbances. There is a need to investigate the impact on anesthesia and intensive care doctors (ICU), especially after the rise of vaccination. Anesthesia and ICU doctors are among the frontline HCW dealing with suspected and confirmed COVID-19 patients. Their job puts them at risk of developing psychological disorders because of the daily stress. The aim of the current study was to assess factors affecting anxiety and depression among vaccinated anesthesia and ICU doctors working in United Arab Emirates (UAE). A cross-sectional study targeting vaccinated anesthesia and ICU doctors in UAE was conducted during March 2021. Data were collected using an online questionnaire uploaded to Google Forms including two sections; the first section included question assessing personal data, professional background data, previous COVID-19 diagnosis, and type of vaccine received. The second section of the questionnaire included the Hospital Anxiety and Depression Scale (HADS). Logistic regression analysis was used to assess the association of different factors with anxiety and depression. Adjusted odds ratio (AOR) and 95% confidence intervals (CI) were calculated.

Results
Significantly higher anxiety (46%) and depression (53.6%) were associated with the Pfizer–BioNTech vaccine. The lowest anxiety (23.9%) and depression (21.6%) scores were related to the Sinopharm vaccine. Anxiety was significantly higher for participants previously diagnosed with COVID-19 (AOR = 2.55), and depression was lower for those who deal with COVID-19-positive patients (AOR = 0.28). Anesthesiologists had significantly lower anxiety and depression than those specialized in both anesthesia and ICU (AOR = 0.32 and 0.51)

Conclusions
Previous diagnosis with COVID-19, female gender, and medical comorbidities were associated with high rates of symptoms of anxiety and depression among anesthesia and ICU doctors. Regular monitoring of the mental health impact of COVID-19, especially after the availability of different vaccines, is recommended.
An essential function for autocrine hedgehog signaling in epithelial proliferation and differentiation in the trachea
Authors: Yin Wenguang , Liontos Andreas , Koepke Janine , Ghoul Maroua , Mazzocchi Luciana , Liu Xinyuan , Lu Chunyan , Wu Haoyu , Fysikopoulos Athanasios , Sountoulidis Alexandros , Seeger Werner , Ruppert Clemens , Günther Andreas , Stainier Didier Y. R. , Samakovlis Christos 
Published: February 2022

ABSTRACT
The tracheal epithelium is a primary target for pulmonary diseases as it provides a conduit for air flow between the environment and the lung lobes. The cellular and molecular mechanisms underlying airway epithelial cell proliferation and differentiation remain poorly understood. Hedgehog (HH) signaling orchestrates communication between epithelial and mesenchymal cells in the lung, where it modulates stromal cell proliferation, differentiation and signaling back to the epithelium. Here, we reveal a previously unreported autocrine function of HH signaling in airway epithelial cells. Epithelial cell depletion of the ligand sonic hedgehog (SHH) or its effector smoothened (SMO) causes defects in both epithelial cell proliferation and differentiation. In cultured primary human airway epithelial cells, HH signaling inhibition also hampers cell proliferation and differentiation. Epithelial HH function is mediated, at least in part, through transcriptional activation, as HH signaling inhibition leads to downregulation of cell type-specific transcription factor genes in both the mouse trachea and human airway epithelial cells. These results provide new insights into the role of HH signaling in epithelial cell proliferation and differentiation during airway development.
Notch signaling regulates neural stem cell quiescence entry and exit in Drosophila
Authors: Sood Chhavi , Justis Virginia T. , Doyle Susan E. , Siegrist Sarah E. 
Published: February 2022

ABSTRACT
Stem cells enter and exit quiescence as part of normal developmental programs and to maintain tissue homeostasis in adulthood. Although it is clear that stem cell intrinsic and extrinsic cues, local and systemic, regulate quiescence, it remains unclear whether intrinsic and extrinsic cues coordinate to control quiescence and how cue coordination is achieved. Here, we report that Notch signaling coordinates neuroblast intrinsic temporal programs with extrinsic nutrient cues to regulate quiescence in Drosophila. When Notch activity is reduced, quiescence is delayed or altogether bypassed, with some neuroblasts dividing continuously during the embryonic-to-larval transition. During embryogenesis before quiescence, neuroblasts express Notch and the Notch ligand Delta. After division, Delta is partitioned to adjacent GMC daughters where it transactivates Notch in neuroblasts. Over time, in response to intrinsic temporal cues and increasing numbers of Delta-expressing daughters, neuroblast Notch activity increases, leading to cell cycle exit and consequently, attenuation of Notch pathway activity. Quiescent neuroblasts have low to no active Notch, which is required for exit from quiescence in response to nutrient cues. Thus, Notch signaling coordinates proliferation versus quiescence decisions.
The effects of cGnRH on gonadotropin secretion and hatching traits in Japanese quail
Authors: ÇIFTCI HB , AYGÜN A 
Published: February 2022

The aim of this study was to measure the effect of chicken Gonadotropin Releasing Hormone-I (cGnRH-I) on male fertility potential. Male and female quails were placed in cages(1 male and 5 females) and devoted into four experimental groups: a) control group - no injection, b) negative control group - 200 μl standard saline was injected, c) 5μg cGnRH group - 5μg cGnRH-I was injected and d) 20μg cGnRH group - 20μg cGnRH-I was injected. Each group was consisting of scattered six cages. In each cage only males were subcutaneously injected under the wing (3 injections - 1 week apart from each other). A fourth injection was administered at the end of egg collection and one hour later blood was collected and serum concentrations of Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), prolactin and testosterone were measured. Egg fertility, hatchability and weekly egg production per hen were also measured. Injection of cGnRH-I did not increase pituitary gonadotropin secretion (LH or FSH); however, serum LH concentration non-significantly reduced in negative control group. A significant decrease in serum testosterone concentration was observed in negative control group compared to 20 μg cGnRH injected group. Fertility and hatchability of total set eggs were lower in negative control group compared to other groups. Egg production in control group was significantly decreased, probably due to the non-significant suppression of prolactin. Embryonic mortality (hatchability of fertile eggs) non-significantly increased in control and negative control groups compared to GnRH injected groups. It seems that cGnRH has a positive effect on fertility and hatchability; however, more studies are needed with older males to confirm our findings.
Laboratory protocol for the digital multiplexed gene expression analysis of nasopharyngeal swab samples using the NanoString nCounter system
Authors: García Aranda Marilina , López-Rodríguez Inmaculada , García-Gutiérrez Susana , Padilla-Ruiz Maria , de Luque Vanessa , Hortas Maria Luisa , Diaz Tatiana , Álvarez Martina , Barragan-Mallofret Isabel , Redondo Maximino ,   Research Network on Health Services in Chronic Diseases (Red de Investigación en Servicios Sanitarios y Enfermedades Crónicas, REDISSEC)
Published: February 2022

This paper describes a laboratory protocol to perform the NanoString nCounter Gene Expression Assay from nasopharyngeal swab samples. It is urgently necessary to identify factors related to severe symptoms of respiratory infectious diseases, such as COVID-19, in order to assess the possibility of establishing preventive or preliminary therapeutic measures and to plan the services to be provided on hospital admission. At present, the samples recommended for microbiological diagnosis are those taken from the upper and/or the lower respiratory tract. The NanoString nCounter Gene Expression Assay is a method based on the direct digital detection of mRNA molecules by means of target-specific, colour-coded probe pairs, without the need for mRNA conversion to cDNA by reverse transcription or the amplification of the resulting cDNA by PCR. This platform includes advanced analysis tools that reduce the need for bioinformatics support and also offers reliable sensitivity, reproducibility, technical robustness and utility for clinical application, even in RNA samples of low RNA quality or concentration, such as paraffin-embedded samples. Although the protocols for the analysis of blood or formalin-fixed paraffin-embedded samples are provided by the manufacturer, no corresponding protocol for the analysis of nasopharyngeal swab samples has yet been established. Therefore, the approach we describe could be adopted to determine the expression of target genes in samples obtained from nasopharyngeal swabs using the nCOUNTER technology.
A systematic review of the case findings, testing and management of COVID-19
Authors: Susanna Dewi , Pratiwi Dian , Purnama Sang Gede 
Published: February 2022

Background: Mass testing and adequate management are essential to terminate the spread of coronavirus disease 2019 (COVID-19). This testing is due to the possibility of unidentified cases, especially ones without COVID-19 related symptoms. This review aimed to examine the outcome of the existing studies on the ways of identifying COVID-19 cases, and determine the populations at risk, symptom and diagnostic test management of COVID-19. Methods: The articles reviewed were scientific publications on the PubMed, Science Direct, ProQuest, and Scopus databases. The keywords used to obtain the data were COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and case detection, case management or diagnostic test. We applied the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and Population, Intervention, Control and Outcomes (PICO) approaches. Results: A total of 21 articles from 13 countries met the inclusion criteria and were further analyzed qualitatively. However, 62% of the articles used a rapid antibody test for screening rather than a rapid antigen test. According to the rapid antigen test, 51.3% were positive, with men aged above 50 years recording the highest number of cases. Furthermore, 57.1% of patients were symptomatic, while diagnostic tests' sensitivity and specificity increased to 100% in 14 days after the onset. Conclusions: Real-time polymerase chain reaction (RT-PCR) is recommended by the World Health Organization for detection of COVID-19. Suppose it is unavailable, the rapid antigen test is used as an alternative rather than the rapid antibody test. Diagnosis is expected to be confirmed using the PCR and serological assay to achieve an early diagnosis of COVID-19, according to disease progression, gradual rapid tests can be used, such as rapid antigen in an earlier week and antibody tests confirmed by RT–PCR and serological assay in the second week of COVID-19.
Fluoxetine pharmacokinetics and tissue distribution quantitatively supports a therapeutic role in COVID-19 at a minimum dose of 20 mg per day
Authors: Eugene Andy R. 
Published: February 2022

Background. Various in vitro studies have shown fluoxetine inhibits multiple variants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pathogen causing the coronavirus disease 2019 (COVID-19) worldwide pandemic and multiple observational clinical studies have shown that patients receiving fluoxetine experienced clinical benefit by lowering the risk of intubation and death. The aim of this study is to conduct population pharmacokinetic dosing simulations to quantify the percentage of patients achieving a trough level for the effective concentration resulting in 50% (EC50) and 90% (EC90) inhibition of SARS-CoV-2 as reported in Calu-3 human lung cells. Methods. Pharmacometric parameter estimates used in this study were obtained from the U.S. FDA website from a new drug application for fluoxetine hydrochloride. A population of 1,000 individuals were simulated at standard fluoxetine antidepressant doses (20 mg/day, 30 mg/day, 40 mg/day, 50 mg/day, and 60 mg/day) to estimate the percentage of the patients achieving a trough plasma level for the EC50 and EC90 SARS-CoV-2 inhibition. All analyses and graphing were conducted in R. Results. By day-10 at 20 mg/day 93.2% and 47% of the population will achieve the trough target plasma EC50 and EC90 concentrations, respectively, which translates to a lung tissue distribution coefficient of 60-times higher EC50 (283.6 ng/ml [0.82 mM]) and EC90 (1390.1 ng/ml [4.02 mM]). Further, by day-10 at an ideal dose of 40 mg/day, 99% and 93% of patients will reach the trough EC50 and EC90 concentrations, respectfully. Lastly, only a dose of 60 mg/day will reach the SARS-CoV-2 EC90 inhibitory concentration in the brain. Conclusion. Overall, with a minimum treatment period of 10-days and a minimum dose of 20 mg/day, this study corroborates in vitro studies reporting fluoxetine inhibiting SARS-CoV-2 titers and also multiple observational clinical studies showing therapeutic benefit of fluoxetine in COVID-19 patients.
Ethical considerations of qualitative non-therapeutic research on people with obsessive–compulsive disorder: an overview
Authors: Ali A Hakami Abeer 
Published: February 2022

Introduction: This paper discusses the ethical considerations involved in conducting qualitative non-therapeutic research on people suffering from obsessive–compulsive disorder (OCD), a topic covered in few, if any, prior studies. Methods: The study summarizes in depth the existing literature on ethical aspects of conducting qualitative and nursing research with psychiatric patients. It constructs an overview of the most important points that should be taken into account when conducting non-therapeutic qualitative research on individuals with this disorder. Results: This paper draws attention to three ethical considerations: (1) informed consent; (2) OCD sufferer’s competence to give consent; and (3) aspects of the relationship that arises between the researcher and participant. Conclusions: This paper argues that those with OCD remain capable of giving consent, notwithstanding the continuing debates around their cognitive condition. It also provides an informative overview of aspects concerning their competence and insight, and suggests precautions to be followed when conducting interviews, paying attention to several factors that may determine the nature of the interview. Keywords: qualitative research, non-therapeutic research, ethical considerations, obsessive–compulsive disorder.
The role of oxidative stress markers in Indonesian chronic kidney disease patients: a cross sectional study
Authors: Alsagaff Mochamad Yusuf , Thaha Mochammad , Pikir Budi Susetyo , Susilo Hendri , Wungu Citrawati Dyah Kencono , Suryantoro Satriyo Dwi , Haryati Mutiara Rizky , Ramadhani Ristra , Agustin Esthiningrum Dewi , Putra Muhammad Rifqi Arya , Maiguma Masayuki , Suzuki Yusuke 
Published: February 2022

Background: Several aspects of chronic kidney disease (CKD) such as the incidence rate and mortality rate are concerning. Oxidative stress contributes to progression and mortality in patients with CKD; however, a specific correlation between several markers of oxidative stress and the estimated glomerular filtration rate (eGFR) and albumin-creatinine ratio (ACR) in the Indonesian population has not been sufficiently described yet. Methods: This study was an analytic observational study with a sample of 56 patients with CKD in Universitas Airlangga Hospital, Surabaya, Indonesia, from December 2019 – March 2020. The markers for oxidative stress investigated were urinary 8-hydroxy-2 deoxyguanosine (8-OHdG), serum symmetric dimethylarginine (SDMA) and asymmetric dimethylarginine (ADMA). The correlations between each variable of oxidative stress and CKD were analyzed using Pearson analysis. Results: There was a positive correlation between 8-OHdG and eGFR (p=0.00, r=0.51); however, there was a negative correlation between 8-OHdG and ACR (p=0.025, r=-0.30). SDMA and eGFR showed a negative correlation (p=0.00, r=-0.648), while SDMA and ACR showed a positive correlation (p=0.03, r=0.349). ADMA showed a negative correlation with eGFR (p=0.00, r=-0.476). There were significantly decreased 8-OHdG but increased ADMA and SDMA as the CKD stage progressed (p=0.001, p=0.00, and p = 0.00, respectively). Higher urine 8-OHdG was detected in patients without history of hemodialysis, whereas ADMA and SDMA showed higher value in patients with hemodialysis (p=0.00, p=0.00, and p=0.004, respectively), patients with history of diabetes mellitus (DM) had higher mean 8-OHdG (p 0.000) yet lower serum ADMA and SDMA (p=0.004 and p=0.003, respectively). Conclusions: In patients with CKD in Indonesia, the markers for oxidative stress 8-OHdG, SDMA, and ADMA are correlated with eGFR and ACR levels. There were also significant difference in 8-OHdG, SDMA, and ADMA levels among CKD stages, between dialysis vs non dialysis, and DM vs non DM patients.
Method for Assessing the Balance of Partnership Among Procurement Stakeholders Through Coordination Mechanisms
Authors: Abramova E. 
Published: February 2022

The proposed article presents the results of the author's study of the indicator of partnership balance in relation to the use of coordination mechanisms in procurement activities of supply chain participants.
Based on a series of expert surveys, the author proposed an approach to defining this parameter, taking into account the assessment of the benefits and losses of partnership participants based on the results of the use of coordination mechanisms.
The study was based on a comparative analysis of retrospective data from companies participating in the questionnaire between July and December 2021. Among them are production, freight forwarding enterprises, warehouse operators, wholesale and retail trading companies.
In conclusion, the author made conclusions about the different influence of mechanisms of joint planning, motivation, resource integration on the dynamics of the indicator of balance of partnership in relations between companies and strategic and tactical suppliers.
El tapir, un vecino poco conocido
Authors: Contreras-Moreno Fernando , Reyna-Hurtado Rafael , Méndez-Saint Martin Gabriela , Simá-Pantí David 
Published: February 2022

El Tsíimin vaga libre por la Selva Maya desde hace mucho, con apariencia de elefante, cuerpo de burro y pariente del caballo, vive entre nosotros, pero poco sabemos sobre el.
Results of ‘two-in-one’ single-stage revision total knee arthroplasty for infection with associated bone loss
Authors: Brunt Andrew C. C. , Gillespie Matthew , Holland George , Brenkel Ivan , Walmsley Phil 
Published: February 2022

Aims Periprosthetic joint infection (PJI) occurs in approximately 1% to 2% of total knee arthroplasties (TKA) presenting multiple challenges, such as difficulty in diagnosis, technical complexity, and financial costs. Two-stage exchange is the gold standard for treating PJI but emerging evidence suggests 'two-in-one' single-stage revision as an alternative, delivering comparable outcomes, reduced morbidity, and cost-effectiveness. This study investigates five-year results of modified single-stage revision for treatment of PJI following TKA with bone loss. Methods Patients were identified from prospective data on all TKA patients with PJI following the primary procedure. Inclusion criteria were: revision for PJI with bone loss requiring reconstruction, and a minimum five years’ follow-up. Patients were followed up for recurrent infection and assessment of function. Tools used to assess function were Oxford Knee Score (OKS) and American Knee Society Score (AKSS). Results A total of 24 patients were included with a mean age of 72.7 years (SD 7.6), mean BMI of 33.3 kg/m2 (SD 5.7), and median ASA grade of 2 (interquartile range 2 to 4). Mean time from primary to revision was 3.0 years (10 months to 8.3 years). At revision, six patients had discharging sinus and three patients had negative cultures from tissue samples or aspirates. Two patients developed recurrence of infection: one was treated successfully with antibiotic suppression and one underwent debridement, antibiotics, and implant retention. Mean AKSS scores at two years showed significant improvement from baseline (27.1 (SD 10.2 ) vs 80.3 (SD 14.8); p < 0.001). There was no significant change in mean AKSS scores between two and five years (80.3 (SD 14.8 ) vs 74.1 (SD 19.8); p = 0.109). Five-year OKS scores were not significantly different compared to two-year scores (36.17 (SD 3.7) vs 33.0 (SD 8.5); p = 0.081). Conclusion ‘Two-in-one’ single-stage revision is effective for treating PJI following TKA with bone loss, providing patients with sustained improvements in outcomes and infection clearance up to five years post-procedure. Cite this article: Bone Jt Open 2022;3(2):107–113.