bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antigen receptor‐engineered Tregs inhibit CNS autoimmunity in cell therapy using nonredundant immune mechanisms in mice
Authors: Pohar Jelka , O'Connor Richard , Manfroi Benoît , El‐Behi Mohamed , Jouneau Luc , Boudinot Pierre , Bunse Mario , Uckert Wolfgang , Luka Marine , Ménager Mickael , Liblau Roland , Anderton Stephen M. , Fillatreau Simon 
Published: May 2022

AbstractCD4+FOXP3+ Tregs are currently explored to develop cell therapies against immune‐mediated disorders, with an increasing focus on antigen receptor‐engineered Tregs. Deciphering their mode of action is necessary to identify the strengths and limits of this approach. Here, we addressed this issue in an autoimmune disease of the CNS, EAE. Following disease induction, autoreactive Tregs upregulated LAG‐3 and CTLA‐4 in LNs, while IL‐10 and amphiregulin (AREG) were increased in CNS Tregs. Using genetic approaches, we demonstrated that IL‐10, CTLA‐4, and LAG‐3 were nonredundantly required for the protective function of antigen receptor‐engineered Tregs against EAE in cell therapy whereas AREG was dispensable. Treg‐derived IL‐10 and CTLA‐4 were both required to suppress acute autoreactive CD4+ T‐cell activation, which correlated with disease control. These molecules also affected the accumulation in the recipients of engineered Tregs themselves, underlying complex roles for these molecules. Noteworthy, despite the persistence of the transferred Tregs and their protective effect, autoreactive T cells eventually accumulated in the spleen of treated mice. In conclusion, this study highlights the remarkable power of antigen receptor‐engineered Tregs to appropriately provide multiple suppressive factors nonredundantly necessary to prevent autoimmune attacks.
Preparation of 3‐Alkylidenephthalides: Recent Advances
Authors: Petrignet Julien , Thibonnet Jérôme , Commeiras Laurent , Gueyrard David 
Published: May 2022

Abstract3‐Alkylidenephthalides represent an important class of natural compounds which are also valuable synthetic precursors for the preparation of phthalides. While recent reviews reported the great interest of the phthalides in the field of medicinal chemistry, few reports are mentioning the recent progress for the synthesis of their unsaturated homologous in the position 3. So, the aim of this report is to present the latest progresses (since 2010 and until 2021) in the synthesis of 3‐alkylidenephtalides.
A New Comparative Potentiometric Method for Analysis of Omarigliptin Using Three Different Sensors
Authors: Kelani Khadiga M. , Hegazy Maha A. , Hassan Amal M. , Tantawy Mahmoud A. 
Published: May 2022

AbstractThis work represents first attempt for potentiometric determination of the most recent antidiabetic; omarigliptin. Three sensors, employing potassium tetrakis (p‐chlorophenyl) borate as a lipophilic cation exchanger, were developed and compared. One liquid contact ion‐selective electrode and two carbon paste‐based solid contact ones, plain one and another one modified with polyaniline nanoparticles, were employed. Performances of fabricated sensors were assessed as per IUPAC recommendations. Incorporation of hydrophobic polyaniline nanoparticles as ion‐to‐electron transducer layer at solid contact/ion‐sensitive membrane interface enhanced sensitivity and stability of the third sensor showing LOD of 2.5×10−7 mol L−1 and slope of 58.57 mV decade−1. The three sensors were applied for omarigliptin determination in presence of its degradation products, in dosage form and spiked human plasma.
Fabrication of Au/Fe3O4/RGO based aptasensor for measurement of miRNA‐128, a biomarker for acute lymphoblastic leukemia (ALL)
Authors: Dinani Homayoon Soleimani , Pourmadadi Mehrab , Yazdian Fatemeh , Rashedi Hamid , Ebrahimi Seyed Ali Seyed , Shayeh Javad Shabani , Ghorbani Mehdi 
Published: May 2022

AbstractDue to their high sensitivity, simplicity, portability, self‐contained, and low cost, the development of electrochemical biosensors is a beneficial way to diagnose and anticipate many types of cancers. An electrochemical nanocomposite‐based aptasensor is fabricated for the determination of miRNA‐128 concentration as the acute lymphoblastic leukemia (ALL) biomarker for the first time. The aptamer chains were immobilized on the surface of the glassy carbon electrode (GCE) through gold nanoparticles/magnetite/reduced graphene oxide (AuNPs/Fe3O4/RGO). Fast Fourier transform infrared (FTIR), X‐ray diffraction (XRD), vibrating sample magnetometer (VSM), and transmission electron microscopy (TEM) were used to characterize synthesized nanomaterials. Cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) were used to characterize the modified GCE in both label‐free and labeled methods. The results indicate that the modified working electrode has high selectivity and for miRNA‐128 over other biomolecules. The hexacyanoferrate redox system typically operated at around 0.3 V (vs. Ag/AgCl), and the methylene blue redox system ran at about 0 V, were used as an electrochemical probe. The detection limit and linear detection range for hexacyanoferrate and methylene blue are 0.05346 fM, 0.1–0.9 fM, and 0.005483 fM, 0.01–0.09 fM, respectively. The stability and diffusion control analyses were performed as well. In both label‐free and labeled methods, the modified electron showed high selectivity for miRNA‐128. The use of methylene blue as a safer redox mediator caused miRNA‐128 to be detected with greater accuracy at low potentials in PBS media. The findings also show the substantial improvement in detection limit and linearity by using reduced graphene oxide‐magnetite‐gold nanoparticles that can be verified by comparing with previous studies on the detection of other miRNAs.
Performance analysis of a parabolic trough collector using a heat pipe exchanger
Authors: Boukhalfa Mohammed , Merzouk Mustapha , Kasbadji Merzouk Nachida , Feidt Michel , Blet Nicolas 
Published: May 2022

AbstractIn this article, a detailed performance analysis of a capillary heat pipe coupled with a parabolic trough collector is performed. The influence of different parameters on the overall system's performance was studied namely; beam radiation and the inclination of the PTC. Results showed that using a heat pipe as an absorber improves the performance of the PTC. At Tmax, the coupling of the heat pipe with PTC leads to a 33% increase in the system's global efficiency and a significant reduction in the heat losses by 46.55%. The inclined arrangement configuration improved the global efficiency of the system by 13%. The obtained results were compared with the experimental data provided by the literature and reasonable agreement was observed, thus confirming the model's validity.
An insight into hybrid membrane‐based air conditioning system performance using gray relational analysis methods: Structural versus operational parameters
Authors: Hashemifard Seyed Abdollatif , Abdollahi Farideh , Khosravi Arash , Matsuura Takeshi 
Published: May 2022

AbstractVarious parameters have an impact on the fresh air temperature and input power of a hybrid membrane‐based air conditioning (HMBAC) system, such as pressure ratio, membrane selectivity, membrane permeance, membrane area, air flowrate of the dehumidification unit, and module length, number of fibers, and fiber outer diameter of the humidification unit, which comprise both operational and structural parameters. Therefore, in this study, the effects of these parameters on the system performance are examined separately based on a statistical approach. The importance order of each parameter and its contribution ratio are determined by using Taguchi method and ANOVA analysis. The optimum level for each input parameter is determined using statistical analysis for the fresh air temperature and input power. Then, for the simultaneous minimization of fresh air temperature and input power Taguchi‐gray relational grade (GRG) is used. GRG revealed that membrane selectivity, pressure ratio, and membrane permeance with the contribution ratio of 37.87%, 32.51%, and 10.55%, respectively, are the most critical parameters of the multiperformance of an HMBAC system. Interestingly dehumidification from a humid airflow can be more effective when membranes with low selectivity and high permeability are used. In conclusion, the optimization outputs disclosed that both structural and operational parameters have significant effects on the performance of the HMBAC system and this optimization can be a helpful tool for designing a HMBAC systems to cover the optimal conditions to develop a sustainable membrane‐based air conditioning system.
Figures, landscapes and landsystems: Digital locations, connectivity and communications
Authors: Whalley W. Brian 
Published: May 2022

AbstractCurrent geomorphological research tends to give preference to landforms rather than landscapes. This commentary brings these notions closer together and incorporates varied views of ‘landscape’ (such as landscapes in art and poetry) as well as showing the difficulties of communicating them to audiences wider than geomorphology. The paper promotes the use of decimal Latitude Longitude (dLL) values to assist better location of samples, analyses and especially landforms, to produce ‘information surfaces’ within landscape and landsystem approaches to geomorphology. This will also help the development of geomorphic visual literacy.
ORYX‐MRSI: A fully‐automated open‐source software for proton magnetic resonance spectroscopic imaging data analysis
Authors: Cengiz Sevim , Yildirim Muhammed , Bas Abdullah , Ozturk‐Isik Esin 
Published: May 2022

AbstractProton magnetic resonance spectroscopic imaging (1H‐MRSI) provides a noninvasive, spatially resolved evaluation of brain metabolism. However, there are some limitations of 1H‐MRSI preventing its wider use in the clinics, including the spectral quality issues, partial volume effect and chemical shift artifact. Additionally, it is necessary to create metabolite maps for analyzing spectral data along with other MRI modalities. In this study, a MATLAB‐based open‐source data analysis software for three‐dimensional 1H‐MRSI, called Oryx‐MRSI, which includes modules for visualization of raw 1H‐MRSI data and LCModel outputs, chemical shift correction, tissue fraction calculation, metabolite map production, and registration onto standard MNI152 brain atlas while providing automatic spectral quality control, is presented. Oryx‐MRSI implements region of interest analysis at brain parcellations defined on MNI152 brain atlas. All generated metabolite maps are stored in NIfTI format. Oryx‐MRSI is publicly available at https://github.com/Computational-Imaging-LAB/Oryx-MRSI along with six example datasets.
Modelling the relationships between the yield and evapotranspiration of maize under salinity stress and nitrogen deficiency
Authors: Saeidi Reza , Sotoodehnia Abbas , Ramezani Etedali Hadi 
Published: May 2022

AbstractUnderstanding the intraseasonal sensitivity of crop evapotranspiration (ET) to stresses allows for accurate modelling of crop yield. In this research, the effects of water salinity stress and soil nitrogen deficiency on evapotranspiration (in the growth stages) and maize yield in 2017 and 2018 were investigated. Four treatments of saline water levels of 0.5 (S0), 2.1 (S1), 3.5 (S2) and 5.7 (S3) dS m−1 were combined with four nitrogen fertilization levels of 100% (N0), 75% (N1), 50% (N2) and 25% (N3). The maize ET amounts from the S0N0 to S3N3 treatments decreased from 80 to 55 mm (in the initial stage), 202 to 129 mm (in development), 127 to 80 mm (in the mid) and 14.5 to 10.5 mm (in the late). The dry biomass yield of maize from the S0N0 to S3N3 treatments was equal to 15.3 to 9.23 t.ha−1. The relative yield of maize was modelled by the additive models of Blank, Stewart and Singh and the multipliable models of Jensen, Rao and Minhas. The results showed that the Stewart and Jensen models were selected as the optimal models. By using the optimal models, the relative yield of maize can be modelled based on the evapotranspiration rate (in the growth stages) under salinity stress and nitrogen deficiency.
The psychometric properties of a self‐report inventory of autistic symptoms in individuals with autism spectrum disorder
Authors: Harris Lani L. , Coolidge Frederick L. 
Published: May 2022

AbstractIntroduction and Methods: This study examined 85 parental‐reports with 85 self‐reports of autistic symptoms with the 84‐item Coolidge Autistic Symptom Survey (CASS). The CASS covers all of the diagnostic criteria for autism spectrum disorder (ASD) in the Diagnostic and Statistical Manual of Mental Disorders (DSM‐5), as no studies to date have compared the self‐reports of individuals with autism with parent‐ or significant‐other‐reports of those individuals using the complete DSM‐5. Results: The CASS‐Parental‐Report and CASS‐Self‐Report exhibited excellent internal reliability (α = 0.97 and 0.96, respectively). There was support for the hypothesis that parental‐reports and self‐reports would be similar (r = 0.66, p < 0.001). Discussion: In conclusion, the CASS should be considered a promising tool for assessing autistic symptoms from both parent‐ and self‐perspectives.
Neddylation inactivation affects cell cycle and apoptosis in sheep follicular granulosa cells
Authors: Qin Xiaowei , Dang Wenqing , Yang Xiaofeng , Wang Kai , Kebreab Ermias , Lyu Lihua 
Published: May 2022

AbstractProtein neddylation inactivation is a novel topic in cancer research. However, there are few studies on the mechanism of neddylation underlying the development of sheep follicular granulosa cells (GCs). In this study, the development of follicular GCs in sheep was inactivated by MLN4924, a neddylation‐specific inhibitor, which significantly attenuated the proliferation and cell index of sheep follicular GCs. Further, the inactivation of neddylation by MLN4924 caused the accumulation of the cullin ring ligase (CRLs) substrates Wee1 and c‐Myc, which could upregulate NOXA protein expression. Meanwhile, the B‐cell lymphoma/leukemia 2 (BCL2) family members Bcl‐2 and MCL‐1 were downregulated, subsequently inducing apoptosis in follicular GCs of sheep. Increasing Wee1 levels caused G2/M‐phase arrest. The effects of neddylation inactivation on Akt, the JAK2/STAT3 signaling pathway, and Forkhead box class O(FOXO) family members were evaluated. Neddylation inactivation by MLN4924 increased the levels of phospho‐Akt, JAK2, phospho‐STAT3, and FOXO1 (p < 0.05) and decreased the levels of phospho‐FOXO3a and STAT3 (p < 0.05). In addition, MLN4924 could alter the mitochondrial morphology of GCs, increase cellular glucose utilization and lactate production, increase reactive oxygen species (ROS) generation, and promote sheep follicular GCs glycolysis, thus causing changes in mitochondrial functions. Together, these findings point to an unrecognized role of neddylation in regulating follicular GCs proliferation in sheep.
Age‐stratified adeno‐associated virus serotype 3 neutralizing and total antibody prevalence in hemophilia A patients from India
Authors: Daniel Hubert D.‐J , Kumar Sanjay , Kannangai Rajesh , J. Farzana , Joel Joseph N. , Abraham Aby , Lakshmi Kavitha M. , Agbandje‐McKenna Mavis , Coleman Kirsten E. , Srivastava Arun , Srivastava Alok , Abraham Asha M. 
Published: May 2022

AbstractGene therapy using an adeno‐associated virus (AAV) vector offers a new treatment option for individuals with monogenetic disorders. The major bottleneck is the presence of pre‐existing anti‐AAV antibodies, which impacts its use. Even very low titers of neutralizing antibodies (NAb) to capsids from natural AAV infections have been reported to inhibit the transduction of intravenously administered AAV in animal models and are associated with limited efficacy in human trials. Assessing the level of pre‐existing NAb is important for determining the primary eligibility of patients for AAV vector‐based gene therapy clinical trials. Techniques used to screen AAV‐antibodies include AAV capsid enzyme‐linked immunosorbent assay (ELISA) and transduction inhibition assay (TIA) for detecting total capsid‐binding (TAb) and Nab, respectively. In this study, we screened 521 individuals with hemophilia A from India for TAb and NAb using ELISA and TIA, respectively. The prevalence of TAb and NAb in hemophilia A patients from India were 96% and 77.5%, respectively. There was a significant increase in anti‐AAV3 NAb prevalence with age in the hemophilia A patient group from India. There was a trend in anti‐AAV3 TAb positivity between the pediatric age group (94.4%) and the adult age group (97.4%).
Comparative assessment of a near‐global view of individual cloud types from space‐borne active and passive sensors and ground‐based observations
Authors: Sarkar Thumree , Dey Sagnik , Ganguly Dilip , Di Girolamo Larry , Hong Yulan 
Published: May 2022

AbstractThe World Meteorological Organizations' International Cloud Atlas recognizes 10 basic cloud genera for classifying clouds. Many of these have been used for over 200 years and are based on cloud appearance and base altitude as seen from surface. Over the satellite era, several missions and programs provide public products that classify clouds into these cloud genera. Here, we provide the first comparison of three such satellite climatologies of cloud genera with surface observations. Specifically, we analyse 10 years (2007–2016) of CloudSat, 4 years (2007–2010) of joint CloudSat‐CALIPSO, 13 years (2000–2012) of ISCCP‐H, and 11 years (1998–2008) of the EECRA data between 50°N and 50°S for eight cloud genera. Averaged over this latitude range, the total cloud amounts for these datasets range from 0.56 to 0.65, with Cumulus (Cu) ranging from 0.06 to 0.14; Stratus (St) from 0.14 to 0.38; Altostratus (As) from 0.05 to 0.13; Altocumulus (Ac) from 0.07 to 0.17; Nimbostratus (Ns) from 0.03 to 0.06; Cirrus (Ci) from 0.1 to 0.19; and Deep‐convective (Dc) from 0.01 to 0.04. The largest disagreement among the sensors is observed for Dc cloud with the coefficient of variation of 44%. On the other hand, the cloud datasets show the best agreement for Ci cloud with the coefficient of variation of 24.1%. Regionally, however, the level of agreement and disagreement can vary drastically. For example, in Indian summer monsoon region (ISM 60°–90°E, 10°–30°N) Ci cloud shows a variation of 28%, whereas the Dc cloud shows 16% variation, which is the opposite of their near‐global feature. The observed discrepancies in cloud genera are discussed in terms of observing characteristics, including instrument, methods, and sampling. Greater effort is still required to reduce discrepancies among these datasets, and the assessment provided here can act as a guide for their use in climate studies.
Age‐ and sex‐specific normative values of bone mineral density in the adult glenoid
Authors: Eghbali Pezhman , Becce Fabio , Goetti Patrick , Vauclair Frederic , Farron Alain , Büchler Philippe , Pioletti Dominique , Terrier Alexandre 
Published: May 2022

AbstractThe objective of this study was to determine the normative bone mineral density (BMD) of cortical and trabecular bone regions in the adult glenoid and its dependence on the subject's age and sex. We analyzed computed tomography (CT) scans of 441 shoulders (310 males, 18–69 years) without any signs of glenohumeral joint pathology. Glenoid BMD was automatically quantified in six volumes of interest (VOIs): cortical bone (CO), subchondral cortical plate (SC), subchondral trabecular bone (ST), and three adjacent layers of trabecular bone (T1, T2, and T3). BMD was measured in Hounsfield unit (HU). We evaluated the association between glenoid BMD and sex and age with the Student's t test and Pearson's correlation coefficient (r), respectively. The lambda‐mu‐sigma method was used to determine age‐ and sex‐specific normative values of glenoid BMD in cortical (CO and SC) and trabecular (ST, T1, T2, and T3) bone. Glenoid BMD was higher in males than females, in most age groups and most VOIs. Before 40 years old, the effect of age on BMD was very weak in both males and females. After 40 years old, BMD declined over time in all VOIs. This BMD decline with age was greater in females (cortical: r = −0.45, trabecular: r = −0.41) than in males (cortical: r = −0.30; trabecular: r = −0.32). These normative glenoid BMD values could prove clinically relevant in the diagnosis and management of patients with various shoulder disorders, in particular glenohumeral osteoarthritis and shoulder arthroplasty or shoulder instability, as well as in related research.
Articular geometry can affect joint kinematics, contact mechanics, and implant‐bone micromotion in total ankle arthroplasty
Authors: Zhang Yanwei , Chen Zhenxian , Zhao Dahang , Yu Jian , Ma Xin , Jin Zhongmin 
Published: May 2022

AbstractImplant loosening and bearing surface wear remain the most common failure problems of total ankle arthroplasty (TAA). One of the main factors leading to these problems is the nonphysiologic design of articular surfaces. The goals of this study were to reveal the effects of the anatomical medial‐lateral borders height differences, coronal and sagittal radii on the joint kinematics, contact mechanics, and implant‐bone micromotion in TAA. A previously developed and validated musculoskeletal (MSK) multibody dynamics (MBD) modeling method of TAA based on AnyBody generic MSK MBD model (five simulations for each implant) was used by combining with a finite element analysis. Five ankle implant models with different articular surface morphologies were created according to the anatomic characteristics of Chinese measurement data, marked as Implant A to E. The total ankle forces and motions during walking simulation were predicted by MSK MBD models and the contact mechanics of the bearing surface and the micromotion of the implant‐bone interface of TAA were predicted by FE models. Compared with Implant A, the internal‐external rotation in Implant E increased by 12.14%, the maximum of anterior‐posterior translation in Implant E increased by 5.62%, the maximum reduction of tibial micromotion in Implant E was 59.98%, and for talar, micromotion was 15.36%. The ankle implant with similar anatomic articular surface has the potential to allow patients to recover better motions and reduce the risk of early loosening. This study would provide design guidance for the development of new ankle implants and further advance the development of TAA. Clinical Significance: This study promoted the improvement of ankle implant design and made contributions to improve the service life of ankle implant and patient satisfaction.
Synthesis of Poly(thioester sulfonamide)s via the Ring‐Opening Copolymerization of Cyclic Thioanhydride with N‐Sulfonyl Aziridine Using Mild Phosphazene Base
Authors: Song Peng‐Duo , Xia Lei , Nie Xuan , Chen Guang , Wang Fei , Zhang Ze , Hong Chun‐Yan , You Ye‐Zi 
Published: May 2022

AbstractProviding access to diverse polymer structures is highly desirable, which helps to explore new polymer materials. Poly(thioester sulfonamide)s, combining both the advantages of thioesters and amides, however, are rarely available in polymer chemistry. Here, the ring‐opening copolymerization (ROCOP) of cyclic thioanhydride with N‐sulfonyl aziridine using mild phosphazene base, resulting in well‐defined poly(thioester sulfonamide)s with highly alternative structures, high yields, and controlled molecular weights, is reported. Additionally, benefiting from the mild catalytic process, this ROCOP can be combined with ROCOP of N‐sulfonyl aziridines with cyclic anhydrides to produce novel block copolymers.