bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kavain ablates the radio-resistance of glioblastoma multiforma by targeting LITAF/NF-κB pathway
Authors: Yu Jianzhong , Yuan Fang , Shi Jun , Zhuang Liyuan , Zeng Hui , Ge Lin , Fang Chuantao , Mei Xinyu , Li Hao , Wang Xiongwei 
Published: June 2022

Abstract
Glioblastoma multiforma (GBM) is the most malignant intrinsic tumor of the central nervous system (CNS), with high morbidity of 3.19/100,000 per year and a poor 5-year survival rate (< 5%) in worldwide. Numerous studies have indicated that GBM shows remarkable radioresistance and aggressive recurrence. However, the mechanisms to endow GBM cells with radioresistance are complex and unclear. Cell growth curve and Colony formation assay were used to analyzed radio-resistance of GBM. Immunoprecipitation and immunoblotting experiments were carried out to analyze protein expression and interaction. In the present study, we found that LITAF, lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α factor, is up-regulated both in mRNA and protein in GBM tumors. Meanwhile, we observed that high LITAF expression contributes to radioresistance of GBM cell lines, corroborated by LITAF−/− U87 and LITAF-low DK are more sensitive to radiation than wild type U87 (LITAF-high) both in vitro and in vivo. Furthermore, we demonstrated that kavain, an active constituent of Piper methysticum Forst., effectively ablates U87 cells' radioresistance in a LITAF dependent manner. In mechanism, our results indicated that 1) the elevation of LITAF in GBM cells activates the NF-κB pathway to promote mesenchymal transition, and 2) kavain disturbs STAT6/LITAF protein interaction and then expels LITAF from the nucleus. Therefore, we consider that kavain may be a potential candidate to develop irradiation therapy adjuvant for GBM.
Effects of breast massage by non-medical personnel on the clinical characteristics and treatment of breast abscess
Authors: Gao Haifeng , Wang Jie , Li Yan , Ding Songtao , Zhang Yi , Chen Si , Zhang Hongwei , Liu Hongyan 
Published: June 2022

Abstract
Introduction Breast massage and manual expression of breast milk are recommended for some breastfeeding problems. Aim This retrospective study evaluated the differences in the clinical characteristics and treatment of lactating mothers with breast abscess involving different breast massage participants. Methods Medical records of 654 women with breast abscess in Haidian Maternal and Child Health Hospital were obtained and analyzed. The women were categorized into the study and control groups based on the person who performed the massage (non-medical vs. medical personnel). The difference in the site of breast abscess, type of abscess cavity, necrotic organization inside the abscess cavity, treatment of abscess, and number of punctures treatment was compared. Results The incidences of breast abscess in the nipple areola area, multicavity, and necrotic tissue in the abscess cavity in the study group were higher than in the control group. The mean number of abscess cavities in the two groups was not significantly different. The puncture treatment rate was lower, while the mean puncture number was higher in the study group. Conclusion Breast massage by non-medical personnel resulted in more complex and difficult to treat abscesses, which underscores the need for mothers to be cautious in choosing breast massage providers.
Preliminary Comparative Transcriptome Profiling of Ovary Tissue in White Muscovy Duck with High- and Low-Egg Production
Authors: Ma Ling , Yu Xuan , Wang Zhiying , Wu Junpeng , Li Hongrui , Huang Lihua , Zhang Jianqin 
Published: June 2022

Abstract
Background
The egg-laying rate is an important indicator for evaluating fertility of poultry, in which the growth and development of ovarian granulosa cells are essential for the egg production performance of poultry. In this study, to understand the mRNA expression regulations and pathways of ovarian granulosa cells organization, we sequenced the white Muscovy ducks of different fecundity at egg production peak using RNA-Seq, further, improve the reproduction character and economic efficiency. Three high-yield and three low-yield Muscovy ducks in the peak egg-laying period were selected according to the egg-laying record in the peak egg-laying period. The ovarian tissues of Muscovy ducks were collected, and the differentially expressed genes (DEGs) were screened by RNA-seq analysis. The main signaling pathways were analyzed by Gene Ontology (GO) annotations enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations enrichment, and verified by qRT-PCR.
Results
The results showed that the ratio of sequencing reads generated by high-yield and low-yield white Muscovy ducks to the reference genome of green head ducks ranged from 60.28–65.58%; Further analysis showed 296 differentially expressed genes (DEGs) were screened. Compared with low-yield white Muscovy ducks, 160 genes were up-regulated and 136 genes were down-regulated in high-yield white Muscovy ducks ovary; GO annotations are mainly enriched in oxygen binding and oxygen transport activities at the molecular level, but not in GO Terms of biological processes and cell components; KEGG pathway was enriched in phagosome, cancer proteoglycan pathway, actin cytoskeleton regulation, cell adhesion molecules, Rap1 signaling pathway and PI3K-Akt signaling pathway. PI3K-Akt signaling pathway was closely related to animal reproductive performance; based on the GO terms and KEGG pathways results, seven genes closely related to ovarian granulosa cell differentiation and proliferation were screened and RNA-seq was used., qRT-PCR results showed that the expression trends of seven randomly selected DEGs were consistent with RNA-seq results.
Conclusion
In summary, this study should serve as an important resource revealing a theoretical basis on the molecular regulation mechanism of reproductive traits genes in granulosa cells of white Muscovy duck ovary.
Gasdermin D-deficient mice are hypersensitive to acute kidney injury
Authors: Linkermann Andreas , Tonnus Wulf , Maremonti Francesca , Belavgeni Alexia , Latk Markus , Brucker Anne , von Mässenhausen Anne , Meyer Claudia , Locke Sophie , Gembardt Florian , Beer Kristina , Hugo Christian , Bornstein Stefan , Kusunoki Yoshihiro , Anders Hans-Joachim , Becker Jan , Shao Feng 
Published: June 2022

Abstract
Signaling pathways of regulated necrosis, such as necroptosis and ferroptosis contribute to acute kidney injury (AKI), but the role of pyroptosis is unclear. Pyroptosis is mediated by the pore-forming protein gasdermin D (GSDMD). Here, we report a specific pattern of GSDMD-protein expression in the peritubular compartment of mice that underwent bilateral ischemia and reperfusion injury (IRI). Along similar lines, the GSDMD protein expression in whole kidney lysates increased during the first 84 hours following cisplatin-induced AKI. Importantly, unlike whole kidney lysates, no GSDMD protein expression was detectable in isolated kidney tubules. In IRI and cisplatin-induced AKI, GSDMD-deficient mice exhibited hypersensitivity to injury as assessed by tubular damage, elevated markers of serum urea and serum creatinine. This hypersensitivity was reversed by combined deficiency of GSDMD and the necroptosis mediator mixed lineage kinase domain-like (MLKL). In conclusion, we demonstrate a non-cell autonomous role for GSDMD in protecting the tubular compartment from necroptosis-mediated damage in IRI.
Effectiveness of protocolized management for patients sustaining maxillofacial fracture with massive oronasal bleeding: A single-center experience
Authors: Wu Fang Chi , Hung Kuo Shu , Lin Yu Wen , Sung Kang , Yang Tsung Han , Wang Chih Jung , Yen Yi Ting 
Published: June 2022

Abstract
Background: Maxillofacial fractures can lead to massive oronasal bleeding; however, surgical hemostasis and packing procedures can be challenging owing to complex facial anatomy. Only a few studies investigated maxillofacial fractures with massive oronasal hemorrhage. However, thus far, no studies reported a protocolized management approach for maxillofacial trauma from a single center. This study aimed to evaluate the effectiveness of protocolized management for maxillofacial fractures with oronasal bleeding. Methods: Patients were identified from the National Cheng University Hospital trauma registry from 2010 to 2020. We included patients with a face Abbreviated Injury Scale (AIS) score of ≥ 3 and active oronasal bleeding. Patients’ characteristics were compared between the angiography and non-angiography groups and between survivors and nonsurvivors. Results: Forty-nine patients were included. Among them, 34 (69%) underwent angiography, of whom 21 received arterial embolization. Forty-seven patients (96%) successfully achieved hemostasis by adhering to the treatment protocol at our institution. Compared with the non-angiography group, the angiography group had significantly more patients requiring oral intubation (97% vs. 53%, p<0.001), Glasgow Coma Scale <9 (GCS ; 79% vs. 27%, p<0.001), head AIS >3 (65% vs. 13%, p=0.001), higher Injury Severity Score (ISS; 43 [33-50] vs. 22 [18-27], p<0.001), higher incidence of cardiopulmonary resuscitation (CPR; 41% vs. 0%, p=0.002), higher mortality rate (35% vs. 7%, p=0.043), and more units of packed red blood cells transfused within 24 hours (PRBC; 12 [6-20] vs. 2 [0-4], p<0.001). Nonsurvivors had significantly more patients with hypotension (62% vs. 8%; p<0.001), higher need for CPR (85% vs. 8%; p<0.001), head AIS >3 (92% vs. 33%; p<0.001), skull base fracture (100% vs. 64%; p=0.011), GCS <9 (100% vs. 50%; p=0.003), higher ISS (50 [43-57] vs. 29 [19-48]; p<0.001), and more units of PRBC transfused within 24 hours (18 [13-22] vs. 6 [2-12]; p=0.001) compared with survivors. More patients in the nonsurvivor group underwent angiography (92% vs. 61%; p=0.043). Among embolized vessels, the internal maxillary artery (65%) was the most common bleeding site. Hypoxic encephalopathy accounted for 92% of deaths.Conclusions: Maxillofacial fractures with massive oronasal bleeding are rare, but fatal. Securing the airway as well as early hemostasis is the top priority to salvage such patients. Protocol-guided management is effective in optimizing outcomes in patients with maxillofacial bleeding.
Offline Signature verification : A Systematic Review
Authors: singh Divpreet , Mittal Ajay , Aggarwal Naveen , Kumar Rajesh 
Published: June 2022

Abstract
One of the most often used biometrics for authentication is the individual's signature. There are a variety of methods for determining if a signature is genuine or counterfeit. There are a variety of methods for confirming the legitimacy of a signature in this research. This is the rationale for the use of signature verification in many financial transactions, including mortgages, checks, and so on. Measuring factors like false acceptance, false rejection, and equal error rate may be done in a variety of ways, and those methods are discussed in this work.
Neurotoxic effects of different sizes of plastics (Nano, Micro, and Macro) on juvenile common carp (Cyprinus carpio)
Authors: Hamed Mohamed , Martyniuk Christopher J. , Naguib Mervat , Lee Jae-Seong , Sayed Alaa El-Din H. 
Published: June 2022

Abstract
Using common carp as a model, we assessed the effects of polyethylene (PE) plastics on brain. We measured activity of acetylcholinesterase (AChE), monoamine oxidase (MAO), and the content of nitric oxide (NO) in carp brain following exposure to 100 mg/L of either macroplastics (MaP), microplastics (MPs) or nanoplastic (NPs) for 15 days compared to an unexposed group. Following exposure, each biochemical biomarker was reduced 30-40%, with a higher magnitude of change corresponding to the smaller size of the particles (NPs>MPs>MaPs). In the carp tectum, exposure for 15 days to plastic particles caused varying degrees of necrosis, fibrosis, changes in blood capillaries, tissue detachment, edema, degenerated connective tissues, and necrosis in large cerebellar neurons and ganglion cells. In the carp retina, there was evidence for necrosis, degeneration, vacuolation, and curvature in the inner layer. Here we provide evidence that exposure to plastic particles can be associated with neurotoxicity in common carp.
Process optimisation for the development of giloy (Tinospora cordifolia L.) juice incorporated amla pulp candy
Authors: P Nirmal , Malik Tanu 
Published: June 2022

Abstract
The juice extracted from the giloy (Tinospora cordifolia L.) stem was incorporated with the pulp of amla (Embilica officinalis Gaertn.) for the preparation of value-added nutritive candy. The process optimization was carried out for the prepared amla- giloy (AG) candy using the ‘one factor at a time’ (OFAT) method. Different concentrations of giloy juice (24ml, 48ml, 60ml, 72ml, and 96ml) were mixed with amla pulp for the development of candy and named AG-1, AG-2, AG-3, AG-4, and AG-5, respectively. AG-3 candy(1:1) with 60ml giloy juice and 60ml amla pulp was selected as the best candy for consumption which is rich in ascorbic acid (186.84mg/100g), β-carotene (166.120µg/100g), free radical inhibition rate (23.42%), total phenol content (55.472mg GAE/100g) and showed no growth of microbes in it. The selected AG candy is accepted with respect to the taste, color, and mouthfeel with overall sensory acceptability of 7.53 on the 9-point hedonic scale.
The anticancer effect of honeybee propolis extract on the β-catenin gene expression in the AGS cell line
Authors: Shahbazi Afagh , Deilami Zahra 
Published: June 2022

Abstract
Background and Objective: As the second cause of cancer-related death, gastric cancer (GC) is a major health challenge worldwide. Today, numerous efforts are underway to discover cancer biomarkers and drugs that affect these biomarkers. One of these biomarkers is the β-catenin protein, the expression of which has recently been shown to increase in GC. In addition, many ongoing efforts have focused on finding organic and natural medicines without the side effects of chemotherapy. One of these natural substances is honeybee propolis. In the present study, the effect of this extract was investigated on β-catenin gene expression in AGS cells.Materials and Methods: Propolis extract was applied to AGS cells at doses of 800, 1200, and 2000 μg/ml at intervals of 48 and 72 h. Next, the expression of this gene was evaluated by extracting RNA using the GAPDH as the reference gene. Finally, the difference in the β-catenin expression between the treatment and control groups was examined by data analysis.Results: A significant decrease was observed in the expression of the β-catenin gene in cells treated within 48 h, but no significant difference in the expression was found in the treatment at 72 h. However, the results are promising for the future use of honeybee propolis in cancer treatment in further studies.
Safety and efficacy of 8- and 10-sector ultrasound cyclocoagulation: a retrospective study
Authors: Rong Zhang , Li Fuhua 
Published: June 2022

Abstract
Purpose: To measure the efficacy and safety of ultrasound cycloplasty (UCP) in sectors 8 and 10.Methods: Thirty-four patients with various types and degrees of glaucoma (43 eyes) underwent UCP at the Aier Eye Hospital of Tianjin University. Primary outcomes included intraocular pressure and visual acuity. Secondary outcomes included corneal endothelium, pupil area, cornea (Surface asymmetry index）SAI and(Surface regularity index) SRI , ciliary body detachment, and the safety and efficacy of UCP after trabeculectomy.Results: Twelve months postoperatively, intraocular pressure (IOP) decreased from 28.4 mmHg to 14.4 mmHg (P < 0.05). On average, IOP decreased by 44.7%, and the success rate was 55.8%. At the final follow-up, IOP decreased by 43.46% on average, and the success rate was 62.8%. There was a significant difference in the postoperative pupil area of the eight sectors (P = 0.016) within six months and no significant difference after six months. There was a significant difference in SRI between baseline and one month after UCP (P = 0.016) and no significant difference in the remaining months. There was no significant difference in corneal endothelium from the preoperative baseline. Ciliary body detachment occurred in sectors 8 and 10, and it was removed and reduced one month after surgery. The success rate of UCP after trabeculectomy was 91%, and there were no follicle-related complications.Conclusion: UCP is safe and significantly lowers IOP, with few short-term complications and no long-term complications. It is safe and effective to select sectors 10 and 8 for IOP reduction; however, there is no advantage in IOP reduction compared with sector 6.
RSPO2 is a novel sensitive and specific biomarker in esophageal squamous intraepithelial neoplasia
Authors: Peng Quanzhou , Cao Tianfeng , Ye Zhujia , Wang Jun , Chen Shang , Yu Yanqi , Yu Yingdian , Xue Wenyuan , Chen Zhiwei , Cheng Zhiqiang , Fan Jian-Bing 
Published: June 2022

Abstract
Background: Esophageal squamous intraepithelial neoplasia (ESIN) is considered a precursor lesion of esophageal squamous cell carcinoma (ESCC), High grade ESIN (HGESIN)has much higher risk of progression to ESCC comparing with low grade ESIN(LGESIN). the distinction between squamous cell dysplasia and reactive changes, and HGESIN and LGESIN can sometimes be subjective and challenging. RSPO2 is a member of the R-spondin family of proteins, and was identified as either oncogenes or tumor suppressors depending on the cancer type. Methods: In the current study, we compared the immunohistochemistry(IHC) expression of newly identified marker RSPO2 with p53 and Ki-67 in different esophageal squamous lesions, and to evaluate correlations between their expression levels in these lesions. Results: IHC stains of RSPO2, p53 and Ki-6 were performed on endoscopic submucosal dissection specimens from 71 patients including 96 HGESIN, 50 LGESIN, and 160 fragments of adjacent normal squamous esophageal mucosa. RSPO2 and Ki-67 showed high sensitivity and specificity for diagnosing ESIN from normal squamous epithelium. The sensitivity of high level expression of RSPO2 in distinguishing HGESIN from LGESIN was as high as 97.92%, but specificity was only 72%. The specificity of strong staining of RSPO2 in distinguishing HGESIN from LGESIN was as high as 100%, but the sensitivity was as low as 29.17%. The specificity of high level expression of Ki-67 and p53 in distinguishing HGESIN from LGESIN was 96% and 96% respectively, but sensitivity was reduced to 56.25% and 62.5% respectively. Conclusions: This study demonstrates that both RSPO2 and Ki-67 can be useful in differential diagnosing ESIN.
A Possible Case of Bictegravir-Associated Severe Unconjugated Hyperbilirubinemia
Authors: Parmar Kanak , Mekraksakit Poemlarp , Nugent Kenneth , Nichols Jacob 
Published: June 2022

Abstract
Background: Bictegravir (BIC) co-formulated with emtricitabine (FTC) and tenofovir alafenamide (TAF) is approved by Federal Food and Drug Administration in 2018 for both treatment-naïve and experienced persons living with HIV (PLWH)Case Presentation: A young man with recently diagnosed human immunodeficiency virus (HIV) infection presented with jaundice. Blood work was significant for mild anemia and grade 4 unconjugated hyperbilirubinemia. A comprehensive evaluation for hemolytic anemia failed to reveal any etiology. Other causes of hyperbilirubinemia were negative. Four months prior, patient was started on antiretroviral therapy with a single tablet regimen containing bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF), brand name Biktarvy®, and the medication was suspected to be the cause. The medication was held, and the hyperbilirubinemia improved. Conclusion: Severe hyperbilirubinemia can be found in the patient using BIC/FTC/TAF. The data for this adverse reaction is scarce, and more studies are needed on this possible side effect. The mechanism of unconjugated hyperbilirubinemia by INSTI remains undefined.
ExAdEx: A simple method to maintain human adipose tissue in long-term cultures reveals differential expansion of adipose progenitor cell subpopulations in fibrotic and pro-inflammatory micro-environments.
Authors: DANI Vincent , BRUNI-FAVIER Solène , CHIGNON-SICARD Bérengère , LOUBAT Agnès , DOGLIO Alain , DANI Christian 
Published: June 2022

Abstract

A relevant model to explore how to promote expansion of adipose progenitor cells (APCs) in lean and obese contexts is still lacking, as current adipocyte models have serious limitations. In this work, we describe a novel adipose tissue model, named ExAdEx, that can be obtained from cosmetic surgery wastes. ExAdEx products are adipose tissue units maintaining the characteristics and organization of adipose tissue as it presents in vivo. The model was viable and metabolically active up to two months and could adopt a pathological-like phenotype. It revealed that inflammatory and fibrotic micro-environments regulated expansion of the CD26 APC subpopulation and its CD54 and CD142 APC progenies differentially. The approach used significantly improves the method of generating adipose tissue models and ExAdEx constitutes a relevant model of human adipose tissue that could be used to identify pathways promoting expansion of APCs in physiological and pathological contexts.
Intra- and Interobserver Reliability Analysis of Pediatric Lower Limb Parameters on Digital Long Leg Radiographs
Authors: Braun Sebastian , Brenneis Marco , Holder Jana , Meurer Andrea , Stief Felix 
Published: June 2022

Abstract
Background:Malalignments of the lower extremity are common reasons for orthopedic consultation because it may lead to osteoarthritis in adulthood. An accurate and reliable radiological assessment of lower limb alignment in children and adolescents is essential for clinical decision-making on treatment of limb deformities and for regular control after a surgical intervention.Objective: First, does the analysis of full-length standing anteroposterior radiographs show a good intra- and interobserver reliability? Second, which parameter is most susceptible to observer-dependent errors? Third, what is the Standard Error of Measurement (SEM95%) of the absolute femoral and tibial length?Methods:Two observers evaluated digital radiographs of 144 legs from 36 children and adolescents with pathological valgus alignment before a temporary hemiepiphysiodesis and before implant removal. Parameters included Mechanical Femorotibial Angle (MFA), Mechanical Axis Deviation (MAD), mechanical Lateral Distal Femoral Angle (mLDFA), mechanical Medial Proximal Tibial Angle (mMPTA), mechanical Lateral Proximal Femoral Angle (mLPFA), mechanical Lateral Distal Tibial Angle (mLDTA), Joint Line Convergence Angle (JLCA), femur length, tibial length. Intra- and interobserver reliability (ICC2,1), SEM95% and proportional errors were calculated.Results:The intra- and interobserver reliability for almost all measurements was found to be good to excellent (Intra-ICC2,1: 0.849–0.999; Inter-ICC2,1: 0.864–0.996). The SEM95% of both observers was found to be ±1.39° (MFA), ±3.31mm (MAD), ±1.06° (mLDFA) and ±1.29° (mMPTA). The proportional error of MAD and MFA are comparable (47.29% vs. 46.33%). The relevant knee joint surface angles show a lower proportional error for mLDFA (42.40%) than for mMPTA (51.60%). JLCA has a proportional error of 138%. Furthermore, the SEM95% for the absolute values of the femoral and tibial length were 4.53mm for the femur and 3.12mm for the tibia.Conclusions:In conclusion, a precise malalignment measurement and the knowledge about SEM95% of the respective parameters is crucial for correct surgical or nonsurgical treatment. The susceptibility to error must be taken into account when interpreting malalignment analysis and must be considered when planning a surgical intervention. The results of the present study elucidate that MAD and MFA are equally susceptible to observer-dependent errors. This study shows good to excellent intra- and interobserver ICCs for all leg alignment parameters and joint surface angles, except for JLCA.Trial registration: This study was registered with DRKS (German Clinical Trials Register) under the number DRKS00015053.Level of Evidence: Level I, Diagnostic Study