bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gastric Cancer Secreted miR-214-3p Inhibits the Anti-Angiogenesis Effect of Apatinib by Suppressing Ferroptosis in Vascular Endothelial Cells.
Authors: Ba Yi , wang Tongtong , Yang Changliang , Wang Weixue , Zhang Yan , Zhu Qihang , Zhang Haiyang 
Published: August 2022

Abstract
Different from necrosis, apoptosis, autophagy and other forms of cell death, ferroptosis is a mechanism that catalyzes lipid peroxidation of polyunsaturated fatty acids under the action of iron divalent or lipoxygenase, leading to cell death. Apatinib is currently used in the third-line standard treatment of advanced gastric cancer, targeting the anti-angiogenesis pathway. However, Apatinib-mediated ferroptosis in vascular endothelial cells has not been reported yet. Tumor-secreted exosomes can be taken up into target cells to regulate tumor development, but the mechanism related to vascular endothelial cell ferroptosis has not yet been discovered. Here, we show that exosomes secreted by gastric cancer cells carry miR-214-3p into vascular endothelial cells and directly target zinc finger protein A20 to negatively regulate ACSL4, a key enzyme of lipid peroxidation during ferroptosis, thereby inhibiting ferroptosis in vascular endothelial cells and reducing the efficiency of Apatinib. In conclusion, inhibition of miR-214-3p can increase the sensitivity of vascular endothelial cells to Apatinib, thereby promoting the antiangiogenic effect of Apatinib, suggesting a potential combination therapy for advanced gastric cancer.
pSuc-EDBAM: Predicting lysine succinylation sites in proteins based on ensemble dense blocks and an attention module
Authors: Jia Jianhua , Wu Genqiang , Li Meifang , Qiu Wangren 
Published: August 2022

Abstract
Background
Lysine succinylation is a newly discovered protein post-translational modifications. Predicting succinylation sites helps investigate the metabolic disease treatments. However, the biological experimental approaches are costly and inefficient, it is necessary to develop efficient computational approaches.
Results
In this paper, we proposed a novel predictor based on ensemble dense blocks and an attention module, called as pSuc-EDBAM, which adopted one hot encoding to derive the feature maps of protein sequences. Afterward, the ensemble dense blocks were used to capture feature information at different levels in the process of feature learning. We also introduced an attention module to evaluate the importance degrees of different features. The experimental results show that Acc reaches 74.25%, and MCC reaches 0.2927 on the testing dataset, which suggest that the pSuc-EDBAM outperforms the existing predictors. To make the convenience of the experimental scientists, a user-friendly web server has been established (http://bioinfo.wugenqiang.top/pSuc-EDBAM/), by which the desired results can be easily obtained.
Conclusions
The experimental results of 10-fold cross-validation on the training dataset and independent test on the testing dataset showed that pSuc-EDBAM outperforms the existing succinylation site predictors and can predict potential succinylation sites effectively. The pSuc-EDBAM is feasible and obtains the credible predictive results, which may also provide valuable references for other related research.
A survey of the current situation in Japan with regard to the recruitment of care workers and a study of measures to secure care workers
Authors: Mitsu Hitomi , Amitani Marie , Ibusuki Rie , Mizuma Kimiko , Owaki Tetsuhiro 
Published: August 2022

Abstract
BackgroundJapan has one of the world's lowest birthrates and an ageing population. As the number of elderly people increases, the number of care workers required in all prefectures across Japan is expected to rise until 2040. There are serious shortages, particularly in urban areas. This study investigated and analyzed the policies of prefectures across Japan towards securing nursing care staff, and it sought to clarify the issues related to nursing care in a society with low birthrates and an aging population.MethodsSurvey period: September-October 2020. The Ministry of Health, Labour and Welfare (MHLW), which oversees all aspects of healthcare in Japan, conducted a web-based search to determine the items deemed necessary, referring to its "Measures to Secure Human Resources for Welfare and Nursing Care". The websites of 47 prefectures were accessed, and each of these items was searched on the Internet using the phrase "securing nursing care workers" to see if any of the survey items were among the top 20 search results.ResultsThe percentage of prefectures that use the "nursing care facility certification evaluation system" in the field of the working environment was the lowest. The prefectures that have adopted the nursing care facility certification evaluation system are concentrated in the Kanto and Chubu regions, where the population is concentrated. The number of establishments undergoing "third-party evaluation of welfare services" was high in Tokyo, Kanagawa, and Aichi, which are major cities in Japan.ConclusionThe shortage of nursing care workers is particularly acute in urban areas, making it difficult to secure staff. There is a need for a multi-pronged approach, including better pay for care workers, improved working conditions, and technological innovation.
AVE 0991 suppresses neuroinflammation of Alzheimer's disease by enhancing autophagy in astrocytes
Authors: Deng Yang , Wang Siyu , Wang Qingguang , Xu Zhaohan , Peng Qiang , Chen Shuaiyu , Zhu Lin , Zhang Yingdong , Duan Rui 
Published: August 2022

Abstract
In our previous study, we have shown that AVE 0991, a nonpeptide analogue of Ang-(1–7), ameliorates cognitive decline and inhibits NLRP3 inflammasome of astrocytes in Alzheimer's disease model mice. Additionally, several studies have suggested that activation of autophagy appears to effectively inhibit the progression of neuroinflammation. However, it is unclear whether AVE 0991 can modulate astrocyte autophagy to suppress neuroinflammation in Alzheimer's disease. In our study, we showed that 30 days of intraperitoneal administration of AVE 0991 improved cognitive deficits and neuronal death in APP/PS1 Alzheimer's disease model mice. Moreover, AVE 0991 treatment greatly suppressed astrocyte-mediated inflammation and up-regulated the expression of autophagy. Furthermore, the inhibitory effect of AVE 0991 on the expression of inflammatory factors was reversed by 3-MA, an autophagy inhibitor. These findings suggest that regulation of autophagy is critical for inhibiting astrocyte neuroinflammatory responses and demonstrate a potential neuroprotective mechanism by which AVE 0991 could suppress neuroinflammatory responses by enhancing autophagy.
Evaluation of water quality index, Levels and Human Health Risk of Metals in Packaged Groundwater from Abeokuta and Sagamu, Ogun State Nigeria
Authors: Taiwo Adewale M. , Ogunsola Deborah O. , Babawale Mutiat K. , Isichei Onyinyechukwu T. , Olayinka Sukurat O. , Adeoye Ifeoluwa A. , Adekoya Ganiyat A. , Tayo Olamide E. 
Published: August 2022

Abstract
Groundwater is an important drinking water source in many developing countries of the world. Contamination of this important resource could lead to serious and irreparable health implications for the consumers. This study, therefore, assessed the water quality index (WQI), levels and human health risk of metals in packaged/sachet groundwater collected from Abeokuta and Sagamu, Ogun state Nigeria. One hundred and forty bags of packaged groundwater from different sampling sites were analysed for metals using inductively coupled plasma - optical emission spectrophotometry (ICP-OES). Packaged groundwater data were analysed for simple descriptive and inferential statistics. Human health risk assessment of metals through consumption of packaged groundwater was evaluated for hazard index (HI), hazard quotient (HQ), and cancer risk (CR). Most of the packaged groundwater parameters were within the permissible limits of the World Health Organization except Mn (1118 ± 1719–1727 ± 2218 µg/L) and Fe (491.8 ± 559.1 µg/L) in selected samples. The WQI data (< 100) indicated suitability for drinking purposes, but when regressed against the health risk indicators showed a weak non-significant positive association. The health risk evaluation of metals revealed high HQs > 1.0 for Ca, Mn, Mo, and Cu through consumption packaged groundwater by adults and children at some sampling sites. The CRs for Co and Ni at most monitoring sites indicated values greater than the acceptable limit of 1 x 10− 4, indicating possible cancer development by the consumers.
LncRNA scaRNA2 bridges DNA end-resection to homologous recombination repair mediated chemoradioresistance
Authors: Yang Yanyong , Chen Yuanyuan , Shen Hui , liu tingting , Cao Kun , Wan Zhijie , Du Zhipeng , Wang Hang , Yu Yue , Ma Shengzhe , Li Bailong , Zhang Wei , Cai Jianming , Gao Fu 
Published: August 2022

Abstract
As a potent target for cancer therapy, ATR mainly regulates homologous recombination (HR)-mediated DNA damage repair. Our previous study first identified that ATR binding long noncoding RNA (lncRNA) is necessary for ATR function and promotes cancer resistance. However, most important lncRNAs, as critical factors for ATR activation, are largely unknown. In the present study, scaRNA2, the most enriched ATR-binding lncRNA, was identified and well characterized. ScaRNA2 was found to be essential for HR-mediated DNA damage repair. Furthermore, scaRNA2 knockdown abrogated the mobilization of ATR and its substrates in response to DNA damage. Mechanistically, scaRNA2 was observed to be necessary for Exo1-mediated DNA end resection and bridged the MRN complex to ATR activation. Using cancer cells and a cell-derived xenograft model, we demonstrated that lentivirus-based knockdown of scaRNA2 effectively increases sensitivity to multiple kinds of chemoradiotherapy. Preclinically, knockdown of scaRNA2 improved the therapeutic effects of radiotherapy on patient-derived organoids and xenograft models. Finally, upregulation of scaRNA2 colocalized with ATR was also found in clinical patients who are resistant to radiotherapy based on tumor regression grades. In conclusion, we identified scaRNA2 as the most abundant lncRNA bound to ATR and uncovered its mechanism in bridging DNA end resection to ATR activation, which could be applied as a potent target for combined cancer treatments with chemoradiotherapy.
Predictive model for persistent hypertension after surgical intervention of primary aldosteronism
Authors: Li Zhuoying , He Yunfeng , Zhang Yao , Chen Gang , Zheng Yongbo , Guo Yuan , Quan Zhen , Wu Xiaohou 
Published: August 2022

Abstract
Background: Primary aldosteronism (PA) is one of the most common causes of secondary hypertension and is potentially curable. However, a large number of patients still undergo persistent hypertension (PHT) after unilateral adrenal surgery. This research retrospectively studied the factors associated with this clinical difficulty and established a prediction model for the postoperative PHT; Methods: 353 patients from 2014 to 2021 with PA undergoing unilateral adrenal surgery were enrolled in this study. Clinical and biochemical characteristics were reviewed and the associating factors were examined using univariate and multivariate analysis. A nomogram-based prediction model was established correspondingly; results: 46.2% (163/190) of patients had post-surgical PHT. Multivariate analysis suggested that BMI≥25, diabetes, duration of hypertension, male gender, and ARR were independent predictors of PHT after surgery. The prediction model based on the nomogram showed good discrimination ability (the C index of the training group and the validation group were 0.783 and 0.769, respectively), and the calibration curves were good as well. Clinical usefulness was quantified using the decision curve analysis (DCA); Conclusion: This nomogram is an integration of the clinical and biochemical data of patients before surgery, and is a reliable tool with high accuracy for predicting the postoperative PHT in patients with PA.
Repeatability assessment of anterior segment biometric measurements in myopic patients using a new anterior segment OCT with Placido corneal topography and agreement with a swept-source OCT
Authors: Wang Hao , Zhu Li-Shuang , Pang Chen-Jiu , Fan Qi 
Published: August 2022

Abstract
The precision of anterior segment biometric measurements in eyes has become increasingly important in refractive surgery. The purpose of this study is to assess the repeatability of the automatic measurements provided by a new spectral-domain optical coherence tomograph (SD-OCT)/Placido topographer (MS-39, CSO) and its agreement with a swept-source OCT (SS-OCT) biometer (CASIA SS-1000, Tomey) in patients with myopia. The right eye of 235 subjects was scanned 3 times with both devices. The evaluated parameters included central corneal radius of the steep meridian, central corneal radius of the flat meridian, mean central corneal radius, thinnest corneal thickness, central corneal thickness, anterior chamber depth, corneal volume and diameter. The repeatability of the MS-39 measurements was calculated using intraclass correlation coefficient (ICC), within subject standard deviation, coefficient of repeatability, coefficient of variation and repeated-measures analysis of variance. The agreement between the two devices was evaluated by 95% limits of agreement. The majority of the parameters showed high repeatability. The repeatability of corneal diameter was slightly lower than the other measurements, although the ICC remained high. Agreement with the SS-OCT was good for all parameters assessed. The high repeatability of automatic measurements by the new device supports its clinical application in myopic eyes with myopia, and the good agreement between the two devices indicates they could be used interchangeably for the parameters evaluated.
Evidence of triplinerved cinnamon from the Siwalik (middle Miocene) sediments of Darjeeling foothill and its biogeographic implication: Asian perspective
Authors: Mahato Sumana , Hazra Taposhi , Khan Mahasin Ali 
Published: August 2022

Abstract
Cinnamomum Schaeffer (avocado family Lauraceae), commonly known as a cinnamon tree, is a highly diverse, economically important evergreen element of tropical and sub-tropical regions of the world. In the present work, we have recovered six compressed leaf remains similar to modern leaves of Cinnamomum from the lower part of the Siwalik strata (middle Miocene) of Darjeeling foothills, eastern Himalaya. The recovered fossil specimens are characterized by an elliptic to the ovate lamina, acute to short acuminate apex, round to acute base, and basal as well as suprabasal acrodromous type of primary venation. Based on preserved architectural features, Siwalik fossil leaves are confidently placed under the genus Cinnamomum. However, the recovered Siwalik specimens cannot be traced up to the species level due to a lack of satisfactory macromorphological features (especially tertiary veins) and epidermal anatomy and are designated here as morphotypes. The evidence of current Siwalik specimens and earlier-reported fossil species similar to thermophilic Cinnamomum in appreciable number from other Siwalik localities (Darjeeling, Uttarakhand, and Nepal) suggests that Cinnamomum was a common Siwalik element and the existence of tropical, warm and humid climatic conditions during the Siwalik sedimentation. This finding also represents an important source of data for understanding the evolution, diversification, and paleobiogeographic history of Cinnamomum from an Asian perspective in deep time.
Efficacy and Safety of the Juan Bi Pill Combined with Methotrexate for Treating Active Rheumatoid Arthritis
Authors: Jia Qingyun , Wang Yiru , Sun Dawei , Li Qiang , Mao Jianchun , Xue Luan , Gu Xiaohua , Yu Xiang , Piao Xuemei , Lu Sheng , Shi Qi , Wang Yongjun , Xu Hao , Liang Qianqian 
Published: August 2022

AbstractObjective:Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by swelling and pain in the joints, which eventually lead to joint destruction. The Juan Bi pill is a Chinese medicine that has been widely used to treat RA in China for hundreds of years. This study demonstrated the effect of the Juan Bi pill on the management of active RA.Methods:Participants received a dose (4 g, twice a day p.o.) of the Juan Bi pill or placebo (tid p.o.) for 12 weeks. Participants were required to take the Juan Bi pill for 3 months with follow-up visits at 6 and 12 months, attending seven assessment visits. Participants will undergo disease activity and safety assessments seven times (at baseline and at 1, 2, 3, 6, and 12 months). The primary endpoint was the 28-joint Disease Activity Score (DAS28-ESR and DAS28-CRP). The secondary endpoints included blood sampling for erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), Health Assessment Questionnaire Disability Index, clinical disease activity index (CDAI), visual analog scale, Short Form-36 score, and morning stiffness.Results:Altogether, 213 participants were screened, and 115 participants were randomized into two groups (Juan Bi pill group, n = 57; placebo group, n = 58); four dropped out. After 12 weeks of treatment, the DAS28-ESR and DAS28-CRP scores of the two groups were significantly reduced. The Juan Bi pill group had significantly reduced DAS28-ESR and DAS28-CRP scores and a significantly higher standard rate of the European League Against Rheumatism score than the placebo group (P < 0.05). After treatment, the number of tenderness/swelling joints, CDAI score, and serum ESR and CRP levels in the two groups demonstrated a decreasing trend, which were all reduced compared with those before treatment. In the electrocardiogram, routine blood tests, liver and kidney functions, and other indices before and after treatment, the adverse reaction rate of the two groups did not increase.Conclusions:TheJuan Bi pill combined with methotrexate can effectively reduce disease activity in participants with RA in the active stage, reduce the symptoms of arthritis, and improve the quality of life, while ensuring safety, reliability, and fewer adverse effects.Trial registration: ClinicalTrials.gov, NCT02885597. Registered on 30 August 2016.
Extraction of Partial Discharge Signal in Predominantly VHF Frequency Range in the Presence of Strong Noise in Power Transformer
Authors: Dukanac Djordje 
Published: August 2022

Abstract
This paper provides a detailed analysis of the online detected partial discharge (PD) in the predominantly VHF range in the power transformer during normal operation in the thermal power plant. A standard UHF drain valve sensor is used with the ability to also capture VHF frequencies of received signals. If PD detection is performed during off-line testing, there are excessive costs proportional to the time during which the power transformers are disconnected from the network. For this economic reason, online PD detection techniques are more convenient. The UHF technique has a higher signal-to-noise ratio compared to IEC 60270 and the acoustic method. To accurately determine the strength and waveform of the PD signal, especially if the source position is far from the UHF sensor or if the signal is weak, it is necessary to properly separate the useful part of the recorded signal from the background noise. The criterion for this is that there are no time shifts of the first peaks of the most prominent PD. For that reason, the beginning and the approximate end of PD signal has to be determined. The results show some obvious similarities of PDs in the recorded signals, such as frequency range, duration, repetition rate and the same dominant frequency, which sufficiently indicates that it is the same type of PD.
Open surgery with a lateral neck approach in cases of foreign body impaction that penetrating the neck through the oesophagus: single- center experience
Authors: Xu Feng , Shen Na , Danzheng Liu 
Published: August 2022

Abstract
Background
Because the cases of foreign body impaction penetrating the neck through the oesophagus are quite scare and doctors have not enough therapeutic experience, we aimed to review cases of foreign body impaction penetrating the neck through the oesophagus to analyze the characteristics of these cases. The open surgery skills of the surgeon, the treatment procedure and the surgeons’ experience in the rare diseases were analyzed.
Methods
We collected and analyzed all cases from 2015–2020 in our hospital treated with open surgery through a lateral neck approach for foreign body impaction in the neck penetrating through the cervical esophagus. The surgical area included the neck, super-mediastinum, left subclavian artery. The clinical characteristic of the cases, surgical skill and procedures for fasting and anti-infection treatment after surgery was studied retrospectively. Following up was with telephone communication.
Results
Our series included fifteen cases of neck and mediastinum abscesses, mediastinitis and artery aneurysmal haematoma due to cervical oesophagus foreign bodies. Tenderness in the pre-cervical site was a physical sign for screening, especially the site above the upper suprasternal fossa. All patients underwent neck, superior-mediastinum, or vessel surgeries. Thirteen cases underwent a lateral neck open surgery with the incision including the left side of neck and only two cases were incised from the right side of the neck. Pus was found 3 days after the impaction in one case, the shortest time observed in our series. the oesophageal laceration was only sutured primarily in 5 cases (33.33%) in all fifteen casas. After sufficient drainage (more than 9 days), antibiotic treatment and fasting (normally 2–3 weeks), the patients gradually began changing to solid foods from fluids after complete blood count and oesophageal radiography result confirmations. Through the following up with telephone, we learned that no severe complications occurred, and all the patients have no swallowing dis-function and recovered well.
Conclusion
Cervical oesophagus perforation due to impaction of a foreign body might be liable to occur in the left side of the patient’s neck. Pus and abscess cavity will begin to form after the impaction history of 3 days, so the surgery should be performed as soon as possible after foreign body impaction and penetrating the neck through oesophagus. It had better in 3 days after impaction. The lateral neck approach surgery and the therapeutic procedure introduced in this article are safe and effective treatments for cases of mis-swallowed foreign bodies penetrating the neck through esophagus.
Structure and properties of active biodegradable films based on starch acetate, low-molecular chitosan and polyvinyl alcohol mixtures
Authors: Kochkina Nataliya E. , Butikova Olga A. 
Published: August 2022

Abstract
The aim of this work was to propose new inexpensive compositions based on a mixture of starch acetate (AcSt), low molecular weight chitosan (LwCh), and polyvinyl alcohol (PVA) for preparation of active biodegradable films for various applications. To this end, the influence of biopolymer concentrations in the mixtures on the formed film structures was studied by the X-ray and SEM methods. The water-related, mechanical and UV-protective properties of the films were also evaluated. It was shown that among the studied samples, the films including 18-20 wt% PVA and 20-25 wt% LwCh had the most homogeneous structure and the best tensile strength and elongation at break. An increase in the LwCh concentration to 30 wt% resulted in films with solubility values close to those of edible systems. All the films were found to possess UV-protective properties. A seed germination test showed that the AcSt/LwCh/PVA systems promoted plant growth. Depending on the ratio of polymer components, such films can be recommended for use as food packaging, mulch coatings or pharmaceutical film materials.