bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy
Authors: Ganan-Gomez Irene , Yang Hui , Ma Feiyang , Montalban-Bravo Guillermo , Thongon Natthakan , Marchica Valentina , Richard-Carpentier Guillaume , Chien Kelly , Manyam Ganiraju , Wang Feng , Alfonso Ana , Chen Shuaitong , Class Caleb , Kanagal-Shamanna Rashmi , Ingram Justin P. , Ogoti Yamini , Rose Ashley , Loghavi Sanam , Lockyer Pamela , Cambo Benedetta , Muftuoglu Muharrem , Schneider Sarah , Adema Vera , McLellan Michael , Garza John , Marchesini Matteo , Giuliani Nicola , Pellegrini Matteo , Wang Jing , Walker Jason , Li Ziyi , Takahashi Koichi , Leverson Joel D. , Bueso-Ramos Carlos , Andreeff Michael , Clise-Dwyer Karen , Garcia-Manero Guillermo , Colla Simona 
Published: March 2022

AbstractMyelodysplastic syndromes (MDS) are heterogeneous neoplastic disorders of hematopoietic stem cells (HSCs). The current standard of care for patients with MDS is hypomethylating agent (HMA)-based therapy; however, almost 50% of MDS patients fail HMA therapy and progress to acute myeloid leukemia, facing a dismal prognosis due to lack of approved second-line treatment options. As cancer stem cells are the seeds of disease progression, we investigated the biological properties of the MDS HSCs that drive disease evolution, seeking to uncover vulnerabilities that could be therapeutically exploited. Through integrative molecular profiling of HSCs and progenitor cells in large patient cohorts, we found that MDS HSCs in two distinct differentiation states are maintained throughout the clinical course of the disease, and expand at progression, depending on recurrent activation of the anti-apoptotic regulator BCL-2 or nuclear factor-kappa B-mediated survival pathways. Pharmacologically inhibiting these pathways depleted MDS HSCs and reduced tumor burden in experimental systems. Further, patients with MDS who progressed after failure to frontline HMA therapy and whose HSCs upregulated BCL-2 achieved improved clinical responses to venetoclax-based therapy in the clinical setting. Overall, our study uncovers that HSC architectures in MDS are potential predictive biomarkers to guide second-line treatments after HMA failure. These findings warrant further investigation of HSC-specific survival pathways to identify new therapeutic targets of clinical potential in MDS.
Changes in the excitability of primary hippocampal neurons following exposure to 3.0 GHz radiofrequency electromagnetic fields
Authors: Echchgadda Ibtissam , Cantu Jody C. , Tolstykh Gleb P. , Butterworth Joseph W. , Payne Jason A. , Ibey Bennett L. 
Published: March 2022

AbstractExposures to radiofrequency electromagnetic fields (RF-EMFs, 100 kHz to 6 GHz) have been associated with both positive and negative effects on cognitive behavior. To elucidate the mechanism of RF-EMF interaction, a few studies have examined its impact on neuronal activity and synaptic plasticity. However, there is still a need for additional basic research that further our understanding of the underlying mechanisms of RF-EMFs on the neuronal system. The present study investigated changes in neuronal activity and synaptic transmission following a 60-min exposure to 3.0 GHz RF-EMF at a low dose (specific absorption rate (SAR) < 1 W/kg). We showed that RF-EMF exposure decreased the amplitude of action potential (AP), depolarized neuronal resting membrane potential (MP), and increased neuronal excitability and synaptic transmission in cultured primary hippocampal neurons (PHNs). The results show that RF-EMF exposure can alter neuronal activity and highlight that more investigations should be performed to fully explore the RF-EMF effects and mechanisms.
Artificial and natural silk materials have high mechanical property variability regardless of sample size
Authors: Greco Gabriele , Mirbaha Hamideh , Schmuck Benjamin , Rising Anna , Pugno Nicola M. 
Published: March 2022

AbstractSilk fibres attract great interest in materials science for their biological and mechanical properties. Hitherto, the mechanical properties of the silk fibres have been explored mainly by tensile tests, which provide information on their strength, Young’s modulus, strain at break and toughness modulus. Several hypotheses have been based on these data, but the intrinsic and often overlooked variability of natural and artificial silk fibres makes it challenging to identify trends and correlations. In this work, we determined the mechanical properties of Bombyx mori cocoon and degummed silk, native spider silk, and artificial spider silk, and compared them with classical commercial carbon fibres using large sample sizes (from 10 to 100 fibres, in total 200 specimens per fibre type). The results confirm a substantial variability of the mechanical properties of silk fibres compared to commercial carbon fibres, as the relative standard deviation for strength and strain at break is 10–50%. Moreover, the variability does not decrease significantly when the number of tested fibres is increased, which was surprising considering the low variability frequently reported for silk fibres in the literature. Based on this, we prove that tensile testing of 10 fibres per type is representative of a silk fibre population. Finally, we show that the ideal shape of the stress–strain curve for spider silk, characterized by a pronounced exponential stiffening regime, occurs in only 25% of all tested spider silk fibres.
MiR-146a alleviates lung injury caused by RSV infection in young rats by targeting TRAF-6 and regulating JNK/ERKMAPK signaling pathways
Authors: Huang Zhi , Liu Xiaoxian , Wu Xi , Chen Min , Yu Wenfeng 
Published: March 2022

AbstractRespiratory syncytial virus (RSV) is a major cause of acute lower respiratory tract infection in infants and children. The present study aimed to investigate the effects of miR-146a on RSV replication and the related mechanisms. Material and methods: We pretreated A549 and HEp-2 cells and young rats with miR-146a mimic before infection with RSV. The expressions of miR-146a and RSV-F mRNA in cells and lung tissues were detected by RT-qPCR, and production of IL-1β, IL-6, IL-18, and TNF-α in bronchial alveolar lavage fluid (BALF) were determined by ELISA. The expression level of TRAF-6 and activation of the JNK/ERK/MAPK/NF-κB signaling pathway was detected by Western blotting. Results: RSV infection significantly reduced miR-146a levels in both A549 and HEp-2 cells and rat lung tissues. RSV infection resulted in accelerated growth, increased release of inflammatory cytokines, increased expression of TRAF-6, and activation of the JNK pathway in cells, and the lung inflammatory infiltration and the pathological score increased in rats. Overexpression of miR-146a targeted down-regulation of TRAF-6 expression and JNK/ERK/MAPK/NF-κB pathway induced by RSV infection, reduced the production of inflammatory cytokines IL-1β, IL-6 and TNF-α, and alleviate lung injury in young rats. We got similar results in both A549 and HEp-2 cell experiments. Conclusion: MiR-146a alleviates lung injury caused by RSV infection in young rats by targeting TRAF-6 and regulating JNK/ERK/MAPK signaling pathways.
Spatial monitoring technologies for coupling the soil plant water animal nexus
Authors: Ashworth Amanda J. , Kharel Tulsi , Sauer Tom , Adams Taylor C. , Philipp Dirk , Thomas Andrew L. , Owens Phillip R. 
Published: March 2022

AbstractSystems-level studies aimed at determining how soil properties are linked to plant production and ultimately animal response spatially are lacking. This study aims to identify if grazing pressure is linked to soil properties, terrain attributes, and above-ground plant accumulation and nutritive value in a silvopastoral (or integrated tree-livestock) system. Overall, cattle prefer grazing native grasses (2.81 vs. 1.24 h ha−1 AU−1) and udic (dry) landscape positions compared to aquic (wet) areas (2.07 vs. 1.60 h ha−1 AU−1). Greater grazing frequency occurs in udic soils with greater phosphorus and potassium contents and with accumulated forage with less lignin (P ≤ 0.05), which correspond to reduced elevation and greater tree height and diameter (shade) during summer mob grazing. Combining spatial monitoring technologies (both soil and animal) with forage allowance can optimize grazing systems management and sustainability spatially and temporally.
Neural oscillation of single silicon nanowire neuron device with no external bias voltage
Authors: Woo Sola , Kim Sangsig 
Published: March 2022

AbstractIn this study, we perform simulations to demonstrate neural oscillations in a single silicon nanowire neuron device comprising a gated p–n–p–n diode structure with no external bias lines. The neuron device emulates a biological neuron using interlinked positive and negative feedback loops, enabling neural oscillations with a high firing frequency of ~ 8 MHz and a low energy consumption of ~ 4.5 × 10−15 J. The neuron device provides a high integration density and low energy consumption for neuromorphic hardware. The periodic and aperiodic patterns of the neural oscillations depend on the amplitudes of the analog and digital input signals. Furthermore, the device characteristics, energy band diagram, and leaky integrate-and-fire operation of the neuron device are discussed.
Quantifying agent impacts on contact sequences in social interactions
Authors: Dekker Mark M. , Blanken Tessa F. , Dablander Fabian , Ou Jiamin , Borsboom Denny , Panja Debabrata 
Published: March 2022

AbstractHuman social behavior plays a crucial role in how pathogens like SARS-CoV-2 or fake news spread in a population. Social interactions determine the contact network among individuals, while spreading, requiring individual-to-individual transmission, takes place on top of the network. Studying the topological aspects of a contact network, therefore, not only has the potential of leading to valuable insights into how the behavior of individuals impacts spreading phenomena, but it may also open up possibilities for devising effective behavioral interventions. Because of the temporal nature of interactions—since the topology of the network, containing who is in contact with whom, when, for how long, and in which precise sequence, varies (rapidly) in time—analyzing them requires developing network methods and metrics that respect temporal variability, in contrast to those developed for static (i.e., time-invariant) networks. Here, by means of event mapping, we propose a method to quantify how quickly agents mingle by transforming temporal network data of agent contacts. We define a novel measure called contact sequence centrality, which quantifies the impact of an individual on the contact sequences, reflecting the individual’s behavioral potential for spreading. Comparing contact sequence centrality across agents allows for ranking the impact of agents and identifying potential ‘behavioral super-spreaders’. The method is applied to social interaction data collected at an art fair in Amsterdam. We relate the measure to the existing network metrics, both temporal and static, and find that (mostly at longer time scales) traditional metrics lose their resemblance to contact sequence centrality. Our work highlights the importance of accounting for the sequential nature of contacts when analyzing social interactions.
Objectively measured physical activity was not associated with neighborhood walkability attributes in community-dwelling patients with stroke
Authors: Kanai Masashi , Izawa Kazuhiro P. , Kubo Hiroki , Nozoe Masafumi , Shimada Shinichi 
Published: March 2022

AbstractAlthough the built environment may affect physical activity, there is little evidence on how neighborhood walkability attributes influence post-stroke physical activity. This study aimed to explore associations between objectively measured physical activity and neighborhood walkability attributes in community-dwelling patients with stroke. This cross-sectional study recruited patients who could ambulate outside free of assistance. We assessed objectively measured physical activity comprising the number of steps taken and time spent in moderate-to-vigorous physical activity (MVPA) with an accelerometer. Neighborhood walkability attributes were evaluated using the Walk Score. Multiple linear regression analyses were used to determine whether the Walk Score was independently associated with the number of steps taken or MVPA. Eighty participants with a mean age of 65.9 ± 11.1 years were included. The participants took an average of 5900.6 ± 2947.3 steps/day and spent an average of 19.7 ± 21.7 min/day in MVPA. The mean Walk Score was 71.4 ± 17.2. Multiple linear regression analyses showed that no significant associations were found between the Walk Score and the number of steps taken or MVPA. No associations were found between objectively measured physical activity and neighborhood walkability attributes in community-dwelling patients with stroke in an Asian area.
Feasibility of substituting old corrugated carton pulp with thermal alkali and enzyme pretreated semichemical mechanical rice straw pulp
Authors: Lai Yu-Hsun , Sun Hao-Chen , Chang Ming-Hui , Li Ching-Chin , Shyu Jiann-Gwo , Perng Yuan-Shing 
Published: March 2022

AbstractIn this study, we separately used a laboratory Hollander beater, a pilot scale 12″ single-disc refiner and an expanded trial with a commercial paper mold mill to investigate the feasibility of using thermal-alkali/enzyme pretreated rice straw semi-chemical mechanical pulp to substitute portions of old corrugated carton board (OCC) pulp in the paper industry. In the laboratory plan, sequential treatments of NaOH at a 5–10% dosage and enzymes at a 0.2–4% dosage were applied to rice straw, followed by beating using a Hollander beater for 1–2 h to complete the rice straw semi-chemical mechanical pulping process. When the NaOH dosage, enzyme dosage and refining time were 10%, 0.2% and 1 h, the best quality rice straw pulp was obtained. Along with the increase in NaOH dosage, the pulp freeness decreased significantly, and the pulp accepted rate increased. Enzymatic treatment enhanced rice straw quality only after NaOH dosage treatment, which then reacted with rice straw to increase the quality of pulp. In the expanded trial, the rice straw semi-chemical mechanical pulp was blended with OCC pulp (0%, 25%, 50%, 75% and 100%) to form handsheets. Along with an increase in rice straw proportions, the tensile index, burst index, and ring-crush index increased by 109–200%, 13–196%, and 124–187%, respectively. In an online commercial paper mold mill trial, blending rice straw pulp with OCC could successfully make paper-mold egg cartons, with both mill operation and product smoothness appearance being highly acceptable.
Assessing the impact of water use in conventional and organic carrot production in Poland
Authors: Kowalczyk Zbigniew , Kuboń Maciej 
Published: March 2022

AbstractAs global water resources are decreasing and the demand for it is constantly increasing, the problem of proper water management is becoming more pressing. Poland is one of the largest producers of vegetables in Europe, including carrots, with significant exports. However its freshwater resources are relatively small. The paper presents the results of research on the water footprint (WF) life cycle assessment (LCA) in conventional and organic carrot production. The methodology of calculating WF was used in accordance with PN-EN ISO 14046. It was found, e.g., that WF for organic production of carrot (WF = 1.9 m3 ha−1) is over five times lower, as compared to conventional production (WF = 10.4 m3 ha−1). In the case of conventional production, the fertilization process (67.0–67.7%) has the greatest impact on the shaping of WF in the individual impact categories, i.e. Human Health, Ecosystem Quality and resources. In organic production, the WF-shaping factor is carrot harvesting (41.9–43.1%). The research can be used to develop pro-ecological carrot production technologies, as well as to shape sustainable development plans in agricultural areas. It can also be used to outline policy directions regarding foreign trade in water-consuming agricultural products.
Grit increases strongly in early childhood and is related to parental background
Authors: Sutter Matthias , Untertrifaller Anna , Zoller Claudia 
Published: March 2022

AbstractGrit has been identified as a very important non-cognitive skill that is positively related to educational achievements and labor market success. Recently, it has also been found to be malleable through interventions in primary schools. Yet, little is still known about its development in early childhood and the influence of family background. We present an experiment with 429 children, aged 3–6 years. We measure the level of grit as children’s perseverance in a real effort task and their willingness to challenge themselves successfully with another, more difficult task. Based on a principal component analysis, we find that grit increases strongly with age. Parents’ assessment of their child’s grit is correlated with the actual behavior of their child. Education of parents plays a role for perseverance. Yet, children’s level of patience is unrelated to their level of grit.
Prognostic significance of platelet-to-albumin ratio in patients with esophageal squamous cell carcinoma receiving definitive radiotherapy
Authors: Huang Zhiyu , Zheng Qunhao , Yu Yilin , Zheng Hongying , Wu Yahua , Wang Zhiping , Liu Lingyun , Zhang Mengyan , Liu Tianxiu , Li Hui , Li Jiancheng 
Published: March 2022

AbstractAccumulating evidence indicates that inflammation and nutrition status are associated with clinical outcomes in patients with various malignancies. This study aimed to evaluate the prognostic significance of the pretreatment platelet to albumin ratio (PAR) in esophageal squamous cell carcinoma (ESCC) patients undergoing definitive radiotherapy. A total of 470 patients who underwent definitive radiotherapy with or without chemotherapy were enrolled. The optimal cut-off values of PAR and other indicators were determined by the X-tile. The Kaplan–Meier method, multivariate analyses Cox regression were conducted to identify the association between those indicators and the survival outcomes. The median follow-up time was 23.5 months. The optimal cut-off value of PAR was 5.7 × 109 and patients were stratified as the low PAR group and the high PAR group. In the univariate analysis, a low overall survival rate was significantly associated with T stage (P = 0.005), TNM stage (P < 0.001), Adjuvant chemotherapy (P = 0.007), neutrophil to lymphocyte ratio (NLR) (P = 0.006), platelet to lymphocyte ratio (P < 0.001), systemic immune-inflammation index (P < 0.001), prognostic nutritional index (P < 0.001) and platelet to albumin ratio (PAR) (P < 0.001). Patients with high PAR were associated with poorer OS and PFS than patients with low PAR. On multivariate analysis, TNM stage (P = 0.001), adjuvant chemotherapy (P < 0.001), and PAR (P = 0.033) were independent prognostic factors in ESCC treated with definitive radiotherapy. PAR is a novel, convenient, and inexpensive prognostic indicator for patients with ESCC undergoing definitive radiotherapy. Future validation from prospective larger-scale studies is warranted.
Unveiling genetic variants for age-related sarcopenia by conducting a genome-wide association study on Korean cohorts
Authors: Jin Heejin , Yoo Hyun Ju , Kim Ye An , Lee Ji Hyun , Lee Young , Kwon Seung-hyun , Seo Young Joo , Lee Seung Hun , Koh Jung-Min , Ji Yunmi , Do Ah Ra , Won Sungho , Seo Je Hyun 
Published: March 2022

AbstractSarcopenia is an age-related disorder characterised by a progressive decrease in skeletal muscle mass. As the genetic biomarkers for sarcopenia are not yet well characterised, this study aimed to investigate the genetic variations related to sarcopenia in a relatively aged cohort, using genome-wide association study (GWAS) meta-analyses of lean body mass (LBM) in 6961 subjects. Two Korean cohorts were analysed, and subgroup GWAS was conducted for appendicular skeletal muscle mass (ASM) and skeletal muscle index. The effects of significant single nucleotide polymorphisms (SNPs) on gene expression were also investigated using multiple expression quantitative trait loci datasets, differentially expressed gene analysis, and gene ontology analyses. Novel genetic biomarkers were identified for LBM (rs1187118; rs3768582) and ASM (rs6772958). Their related genes, includingRPS10,NUDT3,NCF2,SMG7,andARPC5, were differently expressed in skeletal muscle tissue, whileGPD1Lwas not. Furthermore, the ‘mRNA destabilisation’ biological process was enriched for sarcopenia. Our study identifiedRPS10,NUDT3,andGPD1Las significant genetic biomarkers for sarcopenia. These genetic loci were related to lipid and energy metabolism, suggesting that genes involved in metabolic dysregulation may lead to the pathogenesis of age-related sarcopenia.
The hidden community architecture of human brain networks
Authors: Lee Byeongwook , Kang Uiryong , Chang Hongjun , Cho Kwang-Hyun 
Published: March 2022

AbstractThe organizational principles of the community architecture of human brain networks are still mostly unknown. Here, we found that brain networks have a moderate degree of community segregation but are specifically organized to achieve high community overlap while maintaining their segregated community structures. These properties are distinct from other real-world complex networks. Additionally, we found that human subjects with a higher degree of community overlap in their brain networks show greater dynamic reconfiguration and cognitive flexibility.
Analysis of serious adverse events in a pediatric community-acquired pneumonia randomized clinical trial in Malawi
Authors: Ginsburg Amy Sarah , May Susanne 
Published: March 2022

AbstractAmoxicillin is recommended as first-line antibiotic treatment for community-acquired pneumonia, the leading infectious cause of mortality in children aged less than 5 years. We conducted a double-blind, randomized controlled non-inferiority trial comparing 3- to 5-day amoxicillin treatment for non-severe chest-indrawing pneumonia in HIV-negative children aged 2 to 59 months in Malawi. In a secondary analysis, we assessed the frequency of serious adverse events (SAEs) during the trial to evaluate the safety of treatment with amoxicillin. Enrolled children with non-severe chest-indrawing pneumonia were randomized to either 3- or 5-day amoxicillin and followed for 14 days to track clinical outcomes. In addition to evaluation for treatment failure (primary endpoint, day 6), relapse, and study drug adherence, children were assessed for adverse events, including SAEs, which were managed per local standard clinical practice until resolution or stabilization. Between March 2016 and April 2019, 3000 children were enrolled, with male and younger children (aged less than 24 months) demonstrating more SAEs (10.3% for males vs 8.1% for females, p = 0.04; 10.0% for 2–6 months, 10.8% for 7–11 months, 9.7% for 12–23 months and 5.6% for 24–59 months, p = 0.01). The most common SAEs were progression of or recurrent pneumonia (220 SAEs in 217 children), acute gastroenteritis (14 SAEs in 14 children), and fever (8 SAEs in 8 children); however, there were no significant or substantive differences in the percentage of children with pneumonia-related, acute gastroenteritis, or fever SAEs noted between the 3- versus 5-day amoxicillin treatment groups. In our pediatric community-acquired pneumonia trial evaluating amoxicillin treatment, there were relatively few SAEs overall and very few attributed to amoxicillin. Duration of amoxicillin treatment did not impact the frequency of SAEs. We found male and younger children appear to be more vulnerable to SAEs in our trial; however, our data support previous data demonstrating the safety of amoxicillin use in children with pneumonia.Clinical trial registration: ClinicalTrials.gov (NCT02678195).