bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anemia among Pregnant Women in Cambodia: A Descriptive Analysis of Temporal and Geospatial Trends and Logistic Regression-Based Examination of Factors Associated with Anemia in Pregnant Women
Authors: Um Samnang , Sopheab Heng , Yom An , Muir Jonathan A. 
Published: September 2022

ABSTRACTAnemia is a major public health problem for thirty-two million pregnant women worldwide. Anemia during pregnancy is a leading cause of child low birth weight, preterm birth, and perinatal/neonatal mortality. Pregnant women are at higher risk of anemia due to micronutrient deficiencies, hemoglobinopathies, infections, socio-demographic and behavioral factors. This study aimed to: 1) assess temporal and geospatial trends of anemia in Cambodia and 2) identify factors associated with anemia among pregnant women aged 15-49 years old in Cambodia. We analyzed data from the Cambodia Demographic and Health Survey (CDHS) for 2005, 2010, and 2014. Data were pooled across the three survey years for all pregnant women aged 15-49 years. Survey weights were applied to account for the complex survey design of the CDHS. Descriptive statistics were estimated for key sociodemographic characteristics of the study population. We used logistic regressions to assess factors associated with anemia among pregnant women aged 15-49 years old. Anemia in pregnant women aged 15-49 in Cambodia decreased from 56% in 2005 to 53% in 2014. With the highest in Preah Vihear and Stung Treng provinces (74.3%), in Kratie province (73%), and in Prey Veng (65.4%) in 2005, 2010, and 2014 respectively. Compared to pregnant women from the wealthiest households, women from poorest households were more likely to have anemia (AOR = 2.8; 95% CI: 1.76-4.9). Pregnant women from coastal regions were almost twice as likely of having anemia (AOR = 1.9; 95% CI: 1.2-3.0). Pregnant women were more likely anemic if they were in their 2nd trimester (AOR = 2.6; 95% CI: 1.9-3.6) or 3rd trimester (AOR = 1.6 95% CI: 1.2-2.3). Anemia remains highly prevalent among pregnant women in Cambodia. Public health interventions and policies to alleviate anemia should be prioritized and shaped to address these factors
Estimating the health impacts of sugar-sweetened beverage tax for informing policy decisions about the obesity burden in Vietnam
Authors: Nguyen Duyen Thuy , Van Hoang Minh , Dao Son , Do Phuong Hong , Nguyen Quang Dinh , Jewell Jo , Amies-Cull Ben , Muthu Maharajan , Hoang Ly-Na , Le Thu Thi , Nguyen An Thi , Tran Bao Quoc , O’Neill Ciaran 
Published: September 2022

ABSTRACTBackgroundConsidered a “best buy” intervention to cope with the obesity burden, a tax on sugar-sweetened beverages (SSBs) has been adopted in more than 40 countries. In Vietnam, a tax on SSBs has been proposed several times (most recently in 2017). This study aimed to estimate the health impacts of different SSBs tax plans currently under discussion to provide an evidence base to inform decision-making about a SSBs tax policy in Vietnam.MethodFive tax scenarios were modelled, representing three levels of retail price increase: 5%, 11% and 19-20%. Scenarios of the highest price increase were assessed across three different tax designs: ad valorem, volume-based specific tax & sugar based specific tax. In each case we modelled SSBs consumption in each tax scenario; how this reduction in consumption translates to a reduction in total energy intake and how this relationship in turn translates to an average change in body weight and obesity status among adults by applying the calorie-to weight conversion factor. Changes in diabetes type 2 diabetes burden were then calculated based on the change in average body mass index of the modelled cohort. A Monte Carlo simulation approach was applied on the conversion factor of weight change and diabetes risk reduction for the sensitivity analysis.ResultsWhile the impact of a 5% price increase arising from a tax was relatively small, increasing SSBs’ price up to 20% appeared to impact substantially on overweight and obesity rates (reduction of 12.7% and 12.4% respectively) saving 27 million USD for direct medical cost. The greatest reduction in rates was observed for overweight (23≤BMI<25) and obesity grade 1 (25≤BMI<30). The decline in overweight and obesity rates was slightly higher for women than men. Differences were evident across all three tax designs with a specific tax based on sugar density achieving greatest effects.
Single-Molecule Analysis of DNA Base-Stacking Energetics Using Patterned DNA Nanostructures
Authors: Banerjee Abhinav , Anand Micky , Kalita Simanta , Ganji Mahipal 
Published: September 2022

ABSTRACTDNA double helix structure is stabilized by the base-pairing and the base-stacking interactions. Base-stacking interactions originating from hydrophobic interactions between the nucleobases predominantly contribute to the duplex stability. A comprehensive understanding of dinucleotide base-stacking interactions is lacking owing to the unavailability of sensitive techniques that can measure these weak interactions. Earlier studies attempting to address this question only managed to estimate the base-pair stacking interactions, however, disentangling individual base-stacking interactions was enigmatic. By combining multiplexed DNA-PAINT imaging with designer DNA nanostructures, we experimentally measure the free energy of dinucleotide base-stacking at the single-molecule level. Multiplexed imaging enabled us to extract binding kinetics of an imager strand with and without additional dinucleotide stacking interactions in a single imaging experiment, abolishing any effects of experimental variations. The DNA-PAINT data showed that a single additional dinucleotide base-stacking results in as much as 250-fold stabilization of the imager strand binding. We found that the dinucleotide base-stacking energies vary from -1.18 ± 0.17 kcal/mol to -3.57 ± 0.08 kcal/mol for C|T and A|C base-stackings, respectively. We demonstrate the application of base-stacking energetics in designing DNA-PAINT probes for multiplexed super-resolution imaging. Our results will aid in designing functional DNA nanostructures, DNA and RNA aptamers, and facilitate better predictions of the local DNA structure.
Semantic-Specific and Domain-General Mechanisms for Integration and Update of Contextual Information
Authors: Branzi Francesca M. , Lambon Ralph Matthew A. 
Published: September 2022

AbstractRecent research has highlighted the importance of domain-general processes and brain regions for language and semantic cognition. Yet, this has been mainly observed in executively demanding tasks, leaving open the question of the contribution of domain-general processes to natural language and semantic cognition. Using fMRI, we investigated whether neural processes reflecting context integration and context update – two key aspects of naturalistic language and semantic processing – are domain-specific versus domain-general. Thus, we compared neural responses during integration of contextual information across semantic and non-semantic tasks. Whole-brain results revealed both shared (left posterior-dorsal inferior frontal gyrus, left posterior inferior temporal gyrus, and left dorsal angular gyrus/intraparietal sulcus) and distinct (left anterior-ventral inferior frontal gyrus, left anterior ventral angular gyrus, left posterior middle temporal gyrus for semantic control only) regions involved in context integration and update. Furthermore, data-driven functional connectivity analysis clustered domain-specific versus domain-general brain regions into distinct but interacting functional neural networks. These results provide a first characterization of the neural processes required for context-dependent integration during language processing along the domain-specificity dimension, and at the same time, they bring new insights on the role of left posterior lateral temporal cortex and left angular gyrus for semantic cognition.
Dual-Fluorescent Reporter for Live-Cell Imaging of the ER During DENV Infection
Authors: Corliss Lochlain , Holliday Madeline , Lennemann Nicholas J. 
Published: September 2022

AbstractInfection by flaviviruses leads to dramatic remodeling of the endoplasmic reticulum (ER). Viral replication occurs within virus-induced vesicular invaginations in the ER membrane. A hallmark of flavivirus infection is expansion of the ER membrane which can be observed at specific time points post infection. However, this process has not been effectively visualized in living cells throughout the course of infection at the single cell resolution. In this study, we developed a plasmid-based reporter system to monitor flavivirus infection and simultaneous virus-induced manipulation of single cells throughout the course of infection in real-time. This system requires viral protease cleavage to release an ER-anchored fluorescent protein infection reporter that is fused to a nuclear localization signal (NLS). This proteolytic cleavage allows for the translocation of the infection reporter signal to the nucleus while an ER-specific fluorescent marker remains localized in the lumen. Thus, the construct allows for the visualization of virus-dependent changes to the ER throughout the course of infection. In this study, we show that our reporter was efficiently cleaved upon the expression of multiple flavivirus proteases, including dengue virus (DENV), Zika virus (ZIKV), and yellow fever virus (YFV). We also found that the DENV protease-dependent cleavage of our ER-anchored reporter exhibited more stringent cleavage sequence specificity than what has previously been shown with biochemical assays. Using this system for long term time-lapse imaging of living cells infected with DENV, we observed nuclear translocation of the reporter signal beginning approximately 8 hours post-infection, which continued to increase throughout the time course. Interestingly, we found that increased reporter signal translocation correlated with increased ER signal intensity, suggesting a positive association between DENV infection and ER expansion in a time-dependent manner. Overall, this report demonstrates that the FlavER platform provides a useful tool for monitoring flavivirus infection and simultaneously observing virus-dependent changes to the host cell ER, allowing for study of the temporal nature of virus-host interactions.
Serial cross-sectional estimation of vaccine and infection-induced SARS-CoV-2 sero-prevalence in children and adults, British Columbia, Canada: March 2020 to August 2022
Authors: Skowronski Danuta M , Kaweski Samantha E , Irvine Michael A , Kim Shinhye , Chuang Erica SY , Sabaiduc Suzana , Fraser Mieke , Reyes Romina C , Henry Bonnie , Levett Paul N , Petric Martin , Krajden Mel , Sekirov Inna 
Published: September 2022

ABSTRACTBackgroundWe chronicle SARS-CoV-2 sero-prevalence through eight cross-sectional sero-surveys (snapshots) in the Lower Mainland (Greater Vancouver and Fraser Valley), British Columbia, Canada from March 2020 to August 2022.MethodsAnonymized-residual sera were obtained from children and adults attending an outpatient laboratory network. Sera were tested with at least three immuno-assays per snapshot to detect spike (S1) and/or nucleocapsid protein (NP) antibodies. Sero-prevalence was defined by dual-assay positivity, including any or infection-induced, the latter requiring S1+NP antibody detection from January 2021 owing to vaccine availability. Infection-induced estimates were used to assess the extent to which surveillance case reports under-estimated infections.ResultsSero-prevalence was ≤1% by the 3rd snapshot in September 2020 and <5% by January 2021 (4th). Following vaccine roll-out, sero-prevalence increased to >55% by May/June 2021 (5th), ∼80% by September/October 2021 (6th), and >95% by March 2022 (7th). In all age groups, infection-induced sero-prevalence remained <15% through September/October 2021, increasing through subsequent Omicron waves to ∼40% by March 2022 (7th) and ∼60% by July/August 2022 (8th). By August 2022, at least 70-80% of children ≤19 years, 60-70% of adults 20-59 years, but ∼40% of adults ≥60 years had been infected. Surveillance case reports under-estimated infections by 12-fold between the 6th-7th and 92-fold between the 7th-8th snapshots.InterpretationBy August 2022, most children and adults had acquired SARS-CoV-2 vaccine and infection exposures, resulting in more robust hybrid immunity. Conversely the elderly, still at greatest risk of severe outcomes, remain largely-dependent on vaccine-induced protection alone, and should be prioritized for additional doses.
Drosophila Atlastin regulates synaptic vesicle mobilization independent of Bone Morphogenetic Protein signaling
Authors: Bertin Francisca , Jara-Wilde Jorge , Auer Benedikt , Köhler-Solís Andrés , González-Silva Carolina , Thomas Ulrich , Sierralta Jimena 
Published: September 2022

AbstractMotor neurons are highly dependent on membrane trafficking, in which the endoplasmic reticulum (ER) and its contact sites with endosomes, confer the ER the role of a long-distance communicator. Atlastin (Atl), a large GTPase located on the ER membrane is required for its function and its tubular structural dynamics. Atl also downregulates, by a yet unknown mechanism, the BMP (Bone Morphogenic Protein) pathway. In humans, Atl mutations are the second more common cause of Hereditary Spastic Paraplegia (HSP), a genetic disease characterized by spasticity of the lower extremities. Here, we explore the molecular basis of Atl-dependent defects on synaptic vesicle (SV) traffic in Drosophila under the hypothesis that those defects are the direct consequence of the atl-knock-down and not of the Atl-dependent BMP signaling upregulation.Motor neuronal knockdown of atl (Atl-KD) leads to an increase in synaptic and satellite bouton number similar to the increase in BMP signaling activity (TKV-CA). Neuronal Atl-KD also associates to a reduction in the boutons of the abundance of the SV markers CSP (Cysteine string protein) and VGLUT (vesicular glutamate transporter) as well as in TKV-CA larvae, both phenotypes are suppressed by decreasing the function of BMP receptor wishful thinking expressing one copy of the mutant receptor (wit /+). Surprisingly, we determined in Atl-KD larvae an increase in the CSP peripheral density and distribution, dependent on synaptic stimulation, that was not replicated in Tkv-CA larvae, suggesting that there could be differences in the mechanisms that underlie the reduction in CSP abundance. Additionally, we determined that Atl-KD associates to an increase in FM 1-43 unload but not in TKV-CA larvae. Moreover, one copy of wit was not able to suppress the FM-143 in Atl-KD larvae (Atl-KD, wit), supporting that BMP signaling does not participate in this phenotype. Together with the stimuli-dependent changes in the SV distribution and dynamics determined in Atl-KD larvae, we measured an increase in Rab11/CSP colocalization, suggesting changes in SV traffic through late recycling endosomes. Together our results suggest a mechanism by which the loss of an ER structuring protein in the motor neuron could, through its role in regulating SV and endosomal trafficking, explain defects in SV accumulation and synaptic dysfunction.
Perceived barriers and negotiation of using peer-to-peer accommodation by Chinese consumers in the COVID-19 context
Authors: Li Zhiyong , Huang Jiahui , Huang Songshan (Sam) , Huang Dan 
Published: September 2022

Purpose
This study aims to understand Chinese consumers’ perceived barriers to using peer-to-peer (P2P) accommodation before and after the outbreak of COVID-19 and the negotiation strategies they applied in overcoming the barriers and enabling consumption.

Design/methodology/approach
A qualitative research design with 28 semi-structured interviews was used. Data were analysed by content analysis.

Findings
Five psychological barriers and four functional barriers were found to inhibit consumers from using P2P accommodation both before and after the COVID-19 outbreak. In overcoming the perceived barriers, consumers applied both behavioural negotiation strategies, including seeking information, behavioural adaptation, selective choice and seeking social support, and cognitive negotiation strategies, including cognitive adaptation and trusting agents. COVID-19 was found to serve as both a barrier and a facilitator for using P2P accommodation. A barriers–negotiation framework was developed in the context.

Research limitations/implications
Theoretically, this study advances consumer resistance and perceived barriers literature by integrating negotiation and developing a barriers–negotiation framework of P2P accommodation usage. This study also offers insights for practitioners in the P2P accommodation industry.

Originality/value
This study showcases the role of negotiation in understanding barriers to using P2P accommodation, paving the way to extend relevant knowledge to advance consumer resistance research, which is an emerging topic in the broader management domain.
Losses because of risk attitudes or time preference: off-farm cold storage and intertemporal marketing of apple growers in China
Authors: Jin Shaoze , Jia Xiangping , James Harvey S. 
Published: September 2022

PurposeThis paper aims to explore the relationship between prudence in risk attitudes and patience of time preference of Chinese apple growers regarding off-farm cold storage of production and marketing in non-harvest seasons. The authors also consider the effect of farmer participation in cooperative-like organizations known as Farm Bases (FBs).Design/methodology/approachThe authors use multiple list methods and elicitation strategies to measure Chinese apple farmers' risk attitudes and time preferences. Because these farmers can either sell their apples immediately to supermarkets or intermediaries or place them in storage, the authors assess correlations between their storage decisions and their preferences regarding risk and time. The authors also differentiate risks involving gains and losses and empirically examine individual risk attitudes in different scenarios.FindingsMarketing decisions are moderately associated with risk attitudes but not time preference. Farmers with memberships in local farmer cooperatives are likely to speculate more in cold storage. Thus, risk aversion behavioral and psychological motives affect farmers' decision-making of cold storage and intertemporal marketing activities. However, membership in cooperatives does not always result in improved income and welfare for farmers.Research limitations/implicationsThe research confirms that behavioral factors may strongly drive vulnerable smallholder farmers to speculate into storage even under seasonal and uncertain marketing volatility. There is the need to think deeper about the rationale of promoting cooperatives and other agricultural forms, because imposing these without careful consideration can have negative impacts.Originality/valueDo risk and time preferences affect the decision of farmers to utilize storage facilities? This question is important because it is not clear if and how risk preferences affect the tradeoff between consuming today and saving for tomorrow, especially for farmers in developing countries.
Remarkable lives – Laura Scranage in conversation with Robert Hurst
Authors: Scranage Laura , Hurst Robert 
Published: September 2022

Purpose
The purpose of this paper is to share Laura Scranage’s story.

Design/methodology/approach
Laura wrote a short piece detailing her journey and was then interviewed by Robert.

Findings
Laura spoke about the difficulties she has faced in life and how her experiences with horses have been deeply therapeutic.

Research limitations/implications
Recovery narratives such as this give us an overview of only a single person’s experiences. However, they allow the person with lived experience to explore their story in depth.

Practical implications
Laura advocates for more research into how horses can be used in therapeutic interventions.

Social implications
There is so much to learn from a story such as Laura’s, for those who have had similar experiences and for those who work in mental health services.

Originality/value
This is the first time that Laura has chosen to share her unique story.
Determinants of intentions to use foodpanda mobile applications in Bangladesh: the role of attitude and fear of COVID-19
Authors: Hasan Abdulla Al-Towfiq 
Published: September 2022

PurposeThis study investigates the determinants influencing customers’ intentions of using the foodpanda mobile app during COVID-19 pandemic.Design/methodology/approachThis study is based on review of the literatures and 388 usable data collection from respondents having prior experiences in online food delivery (OFD) services in Dhaka city in Bangladesh. The subsequent data is analyzed through Structural Equation Modeling using Smart PLS 3.3.3.FindingsThe findings show that perceived usefulness (PU), ease of use, convenience, attitude and fear of COVID-19 significantly impact intentions to use foodpanda mobile app (ITUFPMA) and attitude. Further, attitude partially mediates between PU, ease of use, convenience and ITUFPMA. Moreover, fear of COVID-19 moderates the strength of association between attitude and ITUFPMA.Practical implicationsThe findings of the study may be useful to OFD services operators in formulating business strategies for improving customers’ foodpanda mobile app use intentions and coping with competitive business environment of sharing economy sector.Originality/valueThe study presents a unique case highlighting what are influencing customer intentions toward OFD services during COVID-19 context. The current study provides important insights for industry operators by integrating PU, ease of use, convenience, attitude and fear of COVID-19 in examining ITUFPMA during COVID-19 situation.
Illiberal economies: ambivalence and critique in an alternative investment scheme
Authors: Hayden Tiana Bakić , Muir Sarah 
Published: September 2022

AbstractThis article examines practices of critique and ambivalence among participants in a controversial Latin American investment scheme called thetelares de la abundancia(‘looms of abundance’). While the dominant discourses about thetelaresconstrue them as predatory, participants understand themselves as knowing subjects who reject a worldview based on economic rationality and utility in favour of one based on emotional connectedness. While this emphasis on emotional connection is not unique to thetelares, what is remarkable is the central role that doubt plays: thetejedoras(weavers), as they call themselves, assert themselves quite explicitly not only as critical and knowing subjects, but also as profoundly ambivalent subjects who insist upon the incommensurability of emotional connection and financial gain even as they simultaneously pursue both. This article explores howtejedorasconfront and deal with their doubts by cultivating practices of ambivalence. In so doing, it foregrounds the multiplicity of people's allegiances and intuitions in contexts marked by competing epistemologies, as is the case not only in Latin America but also in much of the world where illiberalism has become a central concern. Towards that end, the article develops the concept of ‘illiberal economies’ – spaces in which participants simultaneously pursue contradictory and mutually exclusive forms of value – as a way of shedding light on broader political and economic dynamics that can seem simultaneously (and paradoxically) nonsensical and irrational, on the one hand, and manipulative and instrumental, on the other. In so doing, the article foregrounds the ambivalent dynamics that are at the heart of illiberal economies in order to open up analytic possibilities for grappling with illiberalism more broadly.