bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detecting Preseismic Signals in GRACE Gravity Solutions: Application to the 2011 Tohoku Mw 9.0 Earthquake
Authors: Panet Isabelle , Narteau Clément , Lemoine Jean‐Michel , Bonvalot Sylvain , Remy Dominique 
Published: August 2022

AbstractWe conduct a global analysis of GRACE‐reconstructed gravity gradients from July 2004 to February 2011, to test whether the deep signals preceding the March 2011 Tohoku earthquake can be detected before the event as a specific feature originating from solid Earth. First, we improve the angular resolution of the gravity gradients using two overlapping ranges of azimuthal sensitivity to investigate short‐term signals of large amplitude aligned with the orientation of the Northwestern Pacific subduction. Then, we set‐up a method to identify consistent solid Earth signals shared by different GRACE gravity models. Robust signals in a model are selected based on their spatial overlap and relative intensity with the signals of another model, so that their sensitivity to the GRACE data processing and ocean dealiasing product can be tested. We show that the dipolar gravity gradient anomaly before the Tohoku earthquake is nearly unique in space and time in the GRACE GRGS03 solutions. A well‐resolved dipolar spatial pattern, typical of dislocations within the solid Earth and poorly sensitive to the ocean dealiasing model, is detected. In addition, the preseismic gravity gradient increase is highly consistent between the GRGS03 and CSR06 solutions, independently from their respective oceanic corrections, and can be clearly distinguished from rare anomalies of similar amplitudes all associated with the water cycle over continental areas. Our approach offers solutions for the continuous monitoring of the Pacific subduction belt to document transient slabs motions in real time from global satellite gravity fields, and their relation with shallower deformations and seismic events.
Modeling the Influence of Moulin Shape on Subglacial Hydrology
Authors: Trunz Celia , Covington Matthew D. , Poinar Kristin , Andrews Lauren C. , Mejia Jessica , Gulley Jason 
Published: August 2022

AbstractSubglacial models represent moulins as cylinders or cones, but field observations suggest that the upper part of moulins in the Greenland Ice Sheet has more complex shapes. These more complex shapes should cause englacial water storage within moulins to vary as a function of depth, a relationship not currently accounted for in models. Here, we use a coupled englacial‐subglacial channel model to explore how moulin shape affects depth‐dependent moulin water storage and water pressure dynamics within a subglacial channel. We simulate seven different moulin shapes across a range of moulin sizes. We find that the englacial storage capacity at the water level is the main control over the daily water level oscillation range and that depth‐varying changes in englacial water storage control the temporal shape of this oscillation. Further, the cross‐sectional area of the moulin within the daily oscillation range, but not above or below this range, controls pressures within the connected subglacial channel. Specifically, large cross‐sectional areas can dampen daily to weekly oscillations that occur in the surface meltwater supply. Our findings suggest that further knowledge of the shape of moulins around the equilibrium water level would improve englacial storage parameterization in subglacial hydrological models and aid predictions of hydrodynamic coupling.
Demographics of childhood hypertension in the UK: a report from the Southeast England
Authors: Haseler Emily , Singh Cheentan , Newton Joanna , Melhem Nabil , Sinha Manish D. 
Published: August 2022

AbstractWe aimed to describe hypertensive phenotype and demographic characteristics in children and adolescents referred to our paediatric hypertension service. We compared age, ethnicity and BMI in primary hypertension (PH) compared to those with secondary hypertension (SH) and white coat hypertension (WCH). Demographic and anthropometric data were collected for children and adolescents up to age 18 referred to our service for evaluation of suspected hypertension over a 6 year period. Office blood pressure (BP) and out of office BP were performed. Patients were categorised as normotensive (normal office and out of office BP), WCH (abnormal office BP, normal out of office BP), PH (both office and out of office BP abnormal, no underlying cause identified) and SH (both office and out of office BP abnormal, with a secondary cause identified). 548 children and adolescents with mean ± SD age of 10.1 ± 5.8 years and 58.2% girls. Fifty seven percent (n = 314) were hypertensive; of these, 47 (15%), 84 (27%) and 183 (58%) had WCH, PH and SH, respectively. SH presented throughout childhood, whereas PH and WCH peaked in adolescence. Non-White ethnicity was more prevalent within those diagnosed with PH than both the background population and those diagnosed with SH. Higher BMI z-scores were observed in those with PH compared to SH. Hypertensive children <6 years are most likely to have SH and have negligible rates of WCH and PH. PH accounted for 27% of hypertension diagnoses in children and adolescents, with the highest prevalence in adolescence, those of non-White Ethnicity and with excess weight.
Atmospheric chemosynthesis is phylogenetically and geographically widespread and contributes significantly to carbon fixation throughout cold deserts
Authors: Ray Angelique E , Zaugg Julian , Benaud Nicole , Chelliah Devan S , Bay Sean , Wong Hon Lun , Leung Pok Man , Ji Mukan , Terauds Aleks , Montgomery Kate , Greening Chris , Cowan Don A , Kong Weidong , Williams Timothy J , Hugenholtz Philip , Ferrari Belinda C 
Published: August 2022

AbstractCold desert soil microbiomes thrive despite severe moisture and nutrient limitations. In Eastern Antarctic soils, bacterial primary production is supported by trace gas oxidation and the light-independent RuBisCO form IE. This study aims to determine if atmospheric chemosynthesis is widespread within Antarctic, Arctic and Tibetan cold deserts, to identify the breadth of trace gas chemosynthetic taxa and to further characterize the genetic determinants of this process. H2 oxidation was ubiquitous, far exceeding rates reported to fulfill the maintenance needs of similarly structured edaphic microbiomes. Atmospheric chemosynthesis occurred globally, contributing significantly (p &lt; 0.05) to carbon fixation in Antarctica and the high Arctic. Taxonomic and functional analyses were performed upon 18 cold desert metagenomes, 230 dereplicated medium-to-high-quality derived metagenome-assembled genomes (MAGs) and an additional 24,080 publicly available genomes. Hydrogenotrophic and carboxydotrophic growth markers were widespread. RuBisCO IE was discovered to co-occur alongside trace gas oxidation enzymes in representative Chloroflexota, Firmicutes, Deinococcota and Verrucomicrobiota genomes. We identify a novel group of high-affinity [NiFe]-hydrogenases, group 1m, through phylogenetics, gene structure analysis and homology modeling, and reveal substantial genetic diversity within RuBisCO form IE (rbcL1E), and high-affinity 1h and 1l [NiFe]-hydrogenase groups. We conclude that atmospheric chemosynthesis is a globally-distributed phenomenon, extending throughout cold deserts, with significant implications for the global carbon cycle and bacterial survival within environmental reservoirs.
Pathologically high intraocular pressure disturbs normal iron homeostasis and leads to retinal ganglion cell ferroptosis in glaucoma
Authors: Yao Fei , Peng Jingjie , Zhang Endong , Ji Dan , Gao Zhaolin , Tang Yixiong , Yao Xueyan , Xia Xiaobo 
Published: August 2022

AbstractGlaucoma can result in retinal ganglion cell (RGC) death and permanently damaged vision. Pathologically high intraocular pressure (ph-IOP) is the leading cause of damaged vision during glaucoma; however, controlling ph-IOP alone does not entirely prevent the loss of glaucomatous RGCs, and the underlying mechanism remains elusive. In this study, we reported an increase in ferric iron in patients with acute primary angle-closure glaucoma (the most typical glaucoma with ph-IOP damage) compared with the average population by analyzing free iron levels in peripheral serum. Thus, iron metabolism might be involved in regulating the injury of RGCs under ph-IOP. In vitro and in vivo studies confirmed that ph-IOP led to abnormal accumulation of ferrous iron in cells and retinas at 1–8 h post-injury and elevation of ferric iron in serum at 8 h post-injury. Nuclear receptor coactivator 4 (NCOA4)-mediated degradation of ferritin heavy polypeptide 1(FTH1) is essential to disrupt iron metabolism in the retina after ph-IOP injury. Furthermore, knockdown of Ncoa4 in vivo inhibited FTH1 degradation and reduced the retinal ferrous iron level. Elevated ferrous iron induced by ph-IOP led to a marked accumulation of pro-ferroptotic factors (lipid peroxidation and acyl CoA synthetase long-chain family member 4) and a depletion of anti-ferroptotic factors (glutathione, glutathione peroxidase 4, and nicotinamide adenine dinucleotide phosphate). These biochemical changes resulted in RGC ferroptosis. Deferiprone can pass through the blood-retinal barrier after oral administration and chelated abnormally elevated ferrous iron in the retina after ph-IOP injury, thus inhibiting RGC ferroptosis and protecting visual function. In conclusion, this study revealed the role of NCOA4-FTH1-mediated disturbance of iron metabolism and ferroptosis in RGCs during glaucoma. We demonstrate the protective effect of Deferiprone on RGCs via inhibition of ferroptosis, providing a research direction to understand and treat glaucoma via the iron homeostasis and ferroptosis pathways.
Caveolin-1 identified as a key mediator of acute lung injury using bioinformatics and functional research
Authors: Qu Lihua , Li Yi , Chen Chao , Yin Tong , Fang Qian , Zhao Yijin , Lv Wenting , Liu Ziqi , Chen Yangye , Shen Li 
Published: August 2022

AbstractAcute lung injury (ALI) is a potentially life-threatening, devastating disease with an extremely high rate of mortality. The underlying mechanism of ALI is currently unclear. In this study, we aimed to confirm the hub genes associated with ALI and explore their functions and molecular mechanisms using bioinformatics methods. Five microarray datasets available in GEO were used to perform Robust Rank Aggregation (RRA) to identify differentially expressed genes (DEGs) and the key genes were identified via the protein-protein interaction (PPI) network. Lipopolysaccharide intraperitoneal injection was administered to establish an ALI model. Overall, 40 robust DEGs, which are mainly involved in the inflammatory response, protein catabolic process, and NF-κB signaling pathway were identified. Among these DEGs, we identified two genes associated with ALI, of which the CAV-1/NF-κB axis was significantly upregulated in ALI, and was identified as one of the most effective targets for ALI prevention. Subsequently, the expression of CAV-1 was knocked down using AAV-shCAV-1 or CAV-1-siRNA to study its effect on the pathogenesis of ALI in vivo and in vitro. The results of this study indicated that CAV-1/NF-κB axis levels were elevated in vivo and in vitro, accompanied by an increase in lung inflammation and autophagy. The knockdown of CAV-1 may improve ALI. Mechanistically, inflammation was reduced mainly by decreasing the expression levels of CD3 and F4/80, and activating autophagy by inhibiting AKT/mTOR and promoting the AMPK signaling pathway. Taken together, this study provides crucial evidence that CAV-1 knockdown inhibits the occurrence of ALI, suggesting that the CAV-1/NF-κB axis may be a promising therapeutic target for ALI treatment.
Temporal profiling of the breast tumour microenvironment reveals collagen XII as a driver of metastasis
Authors: Papanicolaou Michael , Parker Amelia L. , Yam Michelle , Filipe Elysse C. , Wu Sunny Z. , Chitty Jessica L. , Wyllie Kaitlin , Tran Emmi , Mok Ellie , Nadalini Audrey , Skhinas Joanna N. , Lucas Morghan C. , Herrmann David , Nobis Max , Pereira Brooke A. , Law Andrew M. K. , Castillo Lesley , Murphy Kendelle J. , Zaratzian Anaiis , Hastings Jordan F. , Croucher David R. , Lim Elgene , Oliver Brian G. , Mora Fatima Valdes , Parker Benjamin L. , Gallego-Ortega David , Swarbrick Alexander , O’Toole Sandra , Timpson Paul , Cox Thomas R. 
Published: August 2022

AbstractThe tumour stroma, and in particular the extracellular matrix (ECM), is a salient feature of solid tumours that plays a crucial role in shaping their progression. Many desmoplastic tumours including breast cancer involve the significant accumulation of type I collagen. However, recently it has become clear that the precise distribution and organisation of matrix molecules such as collagen I is equally as important in the tumour as their abundance. Cancer-associated fibroblasts (CAFs) coexist within breast cancer tissues and play both pro- and anti-tumourigenic roles through remodelling the ECM. Here, using temporal proteomic profiling of decellularized tumours, we interrogate the evolving matrisome during breast cancer progression. We identify 4 key matrisomal clusters, and pinpoint collagen type XII as a critical component that regulates collagen type I organisation. Through combining our proteomics with single-cell transcriptomics, and genetic manipulation models, we show how CAF-secreted collagen XII alters collagen I organisation to create a pro-invasive microenvironment supporting metastatic dissemination. Finally, we show in patient cohorts that collagen XII may represent an indicator of breast cancer patients at high risk of metastatic relapse.
Electrically switched underwater capillary adhesion
Authors: Zheng Huanxi , Li Jing , Zhou Yongsen , Zhang Chao , Xu Wanghuai , Deng Yajun , Li Jiaqian , Feng Shile , Yi Zhiran , Zhou Xiaofeng , Ji Xianglin , Shi Peng , Wang Zuankai 
Published: August 2022

AbstractDeveloping underwater adhesives that can rapidly and reversibly switch the adhesion in wet conditions is important in various industrial and biomedical applications. Despite extensive progresses, the manifestation of underwater adhesion with rapid reversibility remains a big challenge. Here, we report a simple strategy that achieves strong underwater adhesion between two surfaces as well as rapid and reversible detachment in on-demand manner. Our approach leverages on the design of patterned hybrid wettability on surfaces that selectively creates a spatially confined integral air shell to preserve the water bridge in underwater environment. The overall adhesion strength can be multiplied by introducing multiple air shells and rapidly broken by disturbing the integrity of the protective air shell in response to the applied voltage on two surfaces. Our design can be constructed on the flexible substrate with hybrid wettability, which can be applied to non-conductive substrates and adapted to more complicated morphologies, extending the choice of underlying materials.
Spatiotemporal dynamics of macrophage heterogeneity and a potential function of Trem2hi macrophages in infarcted hearts
Authors: Jung Seung-Hyun , Hwang Byung-Hee , Shin Sun , Park Eun-Hye , Park Sin-Hee , Kim Chan Woo , Kim Eunmin , Choo Eunho , Choi Ik Jun , Swirski Filip K. , Chang Kiyuk , Chung Yeun-Jun 
Published: August 2022

AbstractHeart failure (HF) is a frequent consequence of myocardial infarction (MI). Identification of the precise, time-dependent composition of inflammatory cells may provide clues for the establishment of new biomarkers and therapeutic approaches targeting post-MI HF. Here, we investigate the spatiotemporal dynamics of MI-associated immune cells in a mouse model of MI using spatial transcriptomics and single-cell RNA-sequencing (scRNA-seq). We identify twelve major immune cell populations; their proportions dynamically change after MI. Macrophages are the most abundant population at all-time points (>60%), except for day 1 post-MI. Trajectory inference analysis shows upregulation of Trem2 expression in macrophages during the late phase post-MI. In vivo injection of soluble Trem2 leads to significant functional and structural improvements in infarcted hearts. Our data contribute to a better understanding of MI-driven immune responses and further investigation to determine the regulatory factors of the Trem2 signaling pathway will aid the development of novel therapeutic strategies for post-MI HF.
Inferring interiors and structural history of top-shaped asteroids from external properties of asteroid (101955) Bennu
Authors: Zhang Yun , Michel Patrick , Barnouin Olivier S. , Roberts James H. , Daly Michael G. , Ballouz Ronald-L. , Walsh Kevin J. , Richardson Derek C. , Hartzell Christine M. , Lauretta Dante S. 
Published: August 2022

AbstractAsteroid interiors play a key role in our understanding of asteroid formation and evolution. As no direct interior probing has been done yet, characterisation of asteroids’ interiors relies on interpretations of external properties. Here we show, by numerical simulations, that the top-shaped rubble-pile asteroid (101955) Bennu’s geophysical response to spinup is highly sensitive to its material strength. This allows us to infer Bennu’s interior properties and provide general implications for top-shaped rubble piles’ structural evolution. We find that low-cohesion (≲0.78 Pa at surface and ≲1.3 Pa inside) and low-friction (friction angle ≲ 35∘) structures with several high-cohesion internal zones can consistently account for all the known geophysical characteristics of Bennu and explain the absence of moons. Furthermore, we reveal the underlying mechanisms that lead to different failure behaviours and identify the reconfiguration pathways of top-shaped asteroids as functions of their structural properties that either facilitate or prevent the formation of moons.
Filming movies of attosecond charge migration in single molecules with high harmonic spectroscopy
Authors: He Lixin , Sun Siqi , Lan Pengfei , He Yanqing , Wang Bincheng , Wang Pu , Zhu Xiaosong , Li Liang , Cao Wei , Lu Peixiang , Lin C. D. 
Published: August 2022

AbstractElectron migration in molecules is the progenitor of chemical reactions and biological functions after light-matter interaction. Following this ultrafast dynamics, however, has been an enduring endeavor. Here we demonstrate that, by using machine learning algorithm to analyze high-order harmonics generated by two-color laser pulses, we are able to retrieve the complex amplitudes and phases of harmonics of single fixed-in-space molecules. These complex dipoles enable us to construct movies of laser-driven electron migration after tunnel ionization of N2and CO2molecules at time steps of 50 attoseconds. Moreover, the angular dependence of the migration dynamics is fully resolved. By examining the movies, we observe that electron holes do not just migrate along the laser polarization direction, but may swirl around the atom centers. Our result establishes a general scheme for studying ultrafast electron dynamics in molecules, paving a way for further advance in tracing and controlling photochemical reactions by femtosecond lasers.
Neutrophils restrain sepsis associated coagulopathy via extracellular vesicles carrying superoxide dismutase 2 in a murine model of lipopolysaccharide induced sepsis
Authors: Bao Wenjie , Xing Huayue , Cao Shiwei , Long Xin , Liu Haifeng , Ma Junwei , Guo Fan , Deng Zimu , Liu Xiaolong 
Published: August 2022

AbstractDisseminated intravascular coagulation (DIC) is a complication of sepsis currently lacking effective therapeutic options. Excessive inflammatory responses are emerging triggers of coagulopathy during sepsis, but the interplay between the immune system and coagulation are not fully understood. Here we utilize a murine model of intraperitoneal lipopolysaccharide stimulation and show neutrophils in the circulation mitigate the occurrence of DIC, preventing subsequent septic death. We show circulating neutrophils release extracellular vesicles containing mitochondria, which contain superoxide dismutase 2 upon exposure to lipopolysaccharide. Extracellular superoxide dismutase 2 is necessary to induce neutrophils’ antithrombotic function by preventing endothelial reactive oxygen species accumulation and alleviating endothelial dysfunction. Intervening endothelial reactive oxygen species accumulation by antioxidants significantly ameliorates disseminated intravascular coagulation improving survival in this murine model of lipopolysaccharide challenge. These findings reveal an interaction between neutrophils and vascular endothelium which critically regulate coagulation in a model of sepsis and may have potential implications for the management of disseminated intravascular coagulation.
Whole blood DNA methylation analysis reveals respiratory environmental traits involved in COVID-19 severity following SARS-CoV-2 infection
Authors: Barturen Guillermo , Carnero-Montoro Elena , Martínez-Bueno Manuel , Rojo-Rello Silvia , Sobrino Beatriz , Porras-Perales Óscar , Alcántara-Domínguez Clara , Bernardo David , Alarcón-Riquelme Marta E. 
Published: August 2022

AbstractSARS-CoV-2 infection can cause an inflammatory syndrome (COVID-19) leading, in many cases, to bilateral pneumonia, severe dyspnea, and in ~5% of these, death. DNA methylation is known to play an important role in the regulation of the immune processes behind COVID-19 progression, however it has not been studied in depth. In this study, we aim to evaluate the implication of DNA methylation in COVID-19 progression by means of a genome-wide DNA methylation analysis combined with DNA genotyping. The results reveal the existence of epigenomic regulation of functional pathways associated with COVID-19 progression and mediated by genetic loci. We find an environmental trait-related signature that discriminates mild from severe cases and regulates, among other cytokines, IL-6 expression via the transcription factor CEBP. The analyses suggest that an interaction between environmental contribution, genetics, and epigenetics might be playing a role in triggering the cytokine storm described in the most severe cases.
Bromo- and iodo-bridged building units in metal-organic frameworks for enhanced carrier transport and CO2 photoreduction by water vapor
Authors: Chen Xinfeng , Peng Chengdong , Dan Wenyan , Yu Long , Wu Yinan , Fei Honghan 
Published: August 2022

AbstractOrganolead halide hybrids have many promising attributes for photocatalysis, e.g. tunable bandgaps and excellent carrier transport, but their instability constraints render them vulnerable to polar molecules and limit their photocatalysis in moisture. Herein, we report the construction of metal–organic frameworks based on [Pb2X]3+ (X = Br–/I–) chains as secondary building units and 2-amino-terephthalate as organic linkers, and extend their applications in photocatalytic CO2 reduction with water vapor as the reductant. Hall effect measurement and ultrafast transient absorption spectroscopy demonstrate the bromo/iodo-bridged frameworks have substantially enhanced photocarrier transport, which results in photocatalytic performances superior to conventional metal-oxo metal-organic frameworks. Moreover, in contrast to lead perovskites, the [Pb2X]3+-based frameworks have accessible porosity and high moisture stability for gas-phase photocatalytic reaction between CO2 and H2O. This work significantly advances the excellent carrier transport of lead perovskites into the field of metal-organic frameworks.