bluuebunny/crossref_metadata_2025_split huggingface dataset:
Avaliação da prevalência e impacto do descalonamento antimicrobiano no tratamento da sepse em pacientes internados em um hospital universitário
Authors: Humacayo Francielle Schremeta , Possagno Gerusa Clazer Halila , Miyoshi Edmar 
Published: November 2022

O objetivo do estudo foi avaliar a prevalência e impacto do descalonamento antimicrobiano realizado no tratamento da sepse em pacientes internados na Unidade de Terapia Intensiva (UTI) de um hospital universitário. Foi um estudo observacional retrospectivo em um hospital universitário do Paraná, Brasil, com dados coletados de prescrições médicas e prontuários eletrônicos. A análise farmacoeconômica utilizou preços disponíveis na lista da CMED. Os resultados foram comparados entre os grupos: descalonamento, escalonamento e manutenção. Entre os 84 pacientes incluídos, o descalonamento foi implementado em 57,2% deles, sendo em 91,7% por estreitamento de espectro. Não houve diferença de mortalidade e tempo de internação em UTI entre os grupos (p > 0,05). O tempo de internação hospitalar foi maior no grupo descalonamento (p= 0,028). O custo médio de tratamento antimicrobiano foi de US$ 547,22 por paciente. Os custos do tratamento dirigido foram menores no grupo descalonamento versus escalonamento (p= 0,001). Não houve diferença significativa no custo total da terapia antimicrobiana entre os grupos descalonamento e escalonamento (p= 0,204). Em 40% dos tratamentos descalonados por estreitamento de espectro foram realizados de forma inadequada. O grupo manutenção apresentou o menor custo final de tratamento. Neste estudo foi demonstrada uma alta prevalência de descalonamento antimicrobiano, sem impacto na mortalidade e tempo de internação em UTI. Apesar de não ter sido encontrado diferença significativa entre os grupos, o grupo descalonamento teve um menor custo total comparado ao grupo escalonamento.
Prophylaxis of helminths in cattle in Brazil
Authors: Carmo Tábata Alves do , Mena Mateus Oliveira , Cipriano Isabela de Almeida , Favare Giordani Mascoli de , Guelpa Gabriel Jabismar , Romão Dayane Sarmento , Dias Yasmin Soares , Pinto Sara da Costa , Fiho Cecílio Viegas Soares , Soutello Ricardo Velludo Gomes de 
Published: November 2022

Cattle ranching in Brazil has long been one of the main and most important sectors of the economy. Among the factors that negatively influence the rearing of production animals, the worm disease deserves prominence, because it is one of the most important problems of the Brazilian herd, being responsible for large losses in livestock activity. Worms are caused by helminths of different phyla, classes, genera and species, and can affect several animals, being extremely important in production animals. These parasites can cause losses and great economic losses due to the fall in animal production and costs with its control. The administration of anthelmintics in the correct dosage is of great importance, because its misuse is probably one of the causes that accelerate the emergence of resistant helminth populations. However, when done incorrectly and indiscriminately, it can lead to inefficiency of the drugs because with the wrong management of these drugs there is a selection of resistant parasites. In view of the above, the objective of this literature review was to gather information on ways to control helminthiases and some alternatives that have been studied to improve livestock production systems. Keywords: Anthelmintics; Helminths; Nematodes.
Panorama de lesões decorrentes de acidentes de trânsito e intervenções fisioterapêuticas
Authors: Silva Ana Caroline Soares da , Gomes João Vitor de Souza , Lopes Millena Bezerra 
Published: November 2022

Esse estudo objetivou compreender os índices a faixa etária das vítimas, e os motivos que levam a ocorrer os acidentes, como infrações, excesso de velocidade, bebidas alcoólicas e os danos que os acidentes de trânsito causam, não só para as vítimas e seus familiares, bem como os danos a saúde pública, de modo que o Brasil ocupa a quinta posição do rankin mundial em acidentes de trânsito, tendo em vista que os indivíduos mais vulneraveis são os motociclistas devido estarem mais expostos ao ambiente, gerando uma considerável demanda por serviços de saúde, acarretando na perda produtiva e altos custos socioeconômicos, observando que as lesões mais comuns foram ortopédicas em membros inferiores e neurológica, como traumatismo crânio encefálico (TCE), que pode ocasionar na incapacidade na realização das atividades de vida diária. Apresenta também a importancia da fisioterapia e as técnicas mais utilizadas, com o intuito de diminuir as possíveis sequelas, o tempo de hospitalização, e reíntegra-lo a sociedade, oferecendo uma melhor qualidade de vida.
Inquérito sorológico de lentivírus de pequenos ruminantes e Toxoplama gondii em caprinos leiteiros de Zabelê, Cariri Ocidental, Paraíba, Brasil
Authors: Alves Renato Vaz , Rizzo Huber , Gaeta Natália Carrillo , Soares Roberto de Castro , Higino Severino Silvano dos Santos , Silva Tatiane Rodrigues da 
Published: November 2022

O objetivo deste estudo foi determinar a ocorrência e os fatores de risco da presença de caprinos soropositivos para lentivírus de pequenos ruminantes (LVPR) e Toxoplasma gondii em criações do município de Zabelê, Paraíba, Brasil. Amostras de sangue foram colhidas em dez rebanhos de caprinos leiteiras, perfazendo um total de 333 animais. O teste de imunodifusão em gel de ágar foi utilizado para determinar a presença de anticorpos contra LVPR, enquanto a reação de imunofluorescência indireta foi utilizada para pesquisa de anticorpos contra T. gondii, sendo considerados positivos títulos acima de 64. Em cada rebanho foi aplicado um questionário com perguntas sobre as características da propriedade, rebanho e tipo de manejo. A frequência de caprinos soropositivos a LVPR foi de 22,2% ± 0,41% (74/333), com focos em 80% (8/10) dos rebanhos. A investigação de anticorpos anti-T. gondii revelou frequência de 3,0% ± 0,22% (10/333), com títulos variando de 64 a 4096, distribuídos em 40% (4/10) dos rebanhos. A coinfecção foi diagnosticada em uma cabra com títulos de 4096 para T. gondii. A análise multivariada indicou que a prática diária de limpeza das instalações reduz o risco de infecção por LVPR em 2,31 vezes (P < 0,001, SD = 0,33). Este estudo, ressalta a necessidade da intensificação das ações voltadas para a melhoria das práticas de manejo sanitário nos rebanhos de caprinos leiterios do município.
Alimentação no tratamento do transtorno de ansiedade
Authors: Lemos Amanda Freitas , Silva Anderson da Silva e , Coutinho Gesiane da Costa , Ferreira José Carlos de Sales , Figueiredo Rebeca Sakamoto 
Published: November 2022

Introdução: O interesse pelo tema surgiu da necessidade de disseminar alimentos e os benefícios e malefícios no tratamento, devido ao aumento expressivo de indivíduos com transtorno de ansiedade. Desse modo, há uma necessidade de buscar dietas que, por meio de alimentos e seus compostos bioativos, possam proporcionar melhoras no comportamento alimentar e no aporte nutricional. Metodologia: De caráter descritivo onde busca-se destacar acerca dos benefícios e malefícios Da alimentação no transtorno de ansiedade registrados na literatura nacional. Resultados: A pesquisa colabora para maiores esclarecimentos dos efeitos da ansiedade em indivíduos adultos. Conclusão: constatou que comportamento alimentar pode ser bastante afetado pelas emoções, visto que as suas escolhas alimentares, as quantidades ingeridas e a frequência das refeições dependem de vários fatores, sendo um deles as emoções e não apenas as suas necessidades fisiológicas e ao se caracterizar a ansiedade e seus sintomas; se enfatiza também sobre a importância do nutricionista na prescrição de alimentos e suplementos no tratamento e prevenção da ansiedade; apontando os efeitos do comportamento alimentar no transtorno de ansiedade.
The lncRNAs involved in regulating the RIG-I signaling pathway
Authors: Liu Jing , Ji Qinglu , Cheng Feng , Chen Dengwang , Geng Tingting , Huang Yueyue , Zhang Jidong , He Yuqi , Song Tao 
Published: November 2022

Understanding the targets and interactions of long non-coding RNAs (lncRNAs) related to the retinoic acid-inducible gene-I (RIG-I) signaling pathway is essential for developing interventions, which would enable directing the host inflammatory response regulation toward protective immunity. In the RIG-I signaling pathway, lncRNAs are involved in the important processes of ubiquitination, phosphorylation, and glycolysis, thus promoting the transport of the interferon regulatory factors 3 and 7 (IRF3 and IRF7) and the nuclear factor kappa B (NF-κB) into the nucleus, and activating recruitment of type I interferons (IFN-I) and inflammatory factors to the antiviral action site. In addition, the RIG-I signaling pathway has recently been reported to contain the targets of coronavirus disease-19 (COVID-19)-related lncRNAs. The molecules in the RIG-I signaling pathway are directly regulated by the lncRNA–microRNAs (miRNAs)–messenger RNA (mRNA) axis. Therefore, targeting this axis has become a novel strategy for the diagnosis and treatment of cancer. In this paper, the studies on the regulation of the RIG-I signaling pathway by lncRNAs during viral infections and cancer are comprehensively analyzed. The aim is to provide a solid foundation of information for conducting further detailed studies on lncRNAs and RIG-I in the future and also contribute to clinical drug development.
Association between serum Klotho levels and the prevalence of diabetes among adults in the United States
Authors: Wang Kai , Mao Yukang , Lu Miao , Liu Xianling , Sun Yan , Li Zhongming , Li Yansong , Ding Yinzhang , Zhang Jing , Hong Jian , Xu Di 
Published: November 2022

BackgroundDiabetes is a critical contributor to the pathogenesis of cardiovascular diseases. Klotho is an anti−aging protein with cardiovascular-renal protective effects. However, the relationship between serum Klotho levels and diabetes remains poorly understood.ObjectivesThis study aimed to investigate the relationship between serum Klotho levels and diabetes in US adults.MethodsWe analyzed the cross-sectional data obtained from 13751 subjects aged 40-79 years in the National Health and Nutrition Examination Survey (NHANES) (2007–2016). Serum Klotho concentration was measured using an enzyme-linked immunosorbent assay (ELISA) and categorized into four quartiles (Q1-Q4). Multivariate logistic regression and restricted cubic spline (RCS) regression were conducted to explore the association between serum Klotho levels and the prevalence of diabetes.ResultsAs compared with quartile 1, serum Klotho levels in quartiles 2-4 yielded odds ratios (OR) (95% CI) of diabetes of 0.96 (0.80–1.15), 0.98 (0.82–1.18), and 1.25 (1.04–1.50), respectively, after covariate adjustment (P for trend = 0.018). The results implied an increased risk of diabetes. The RCS plot showed a U-shaped relationship linking serum Klotho levels with diabetes (P for nonlinearity = 0.003).ConclusionsIn summary, a nonlinear and positive association was found between serum Klotho levels and the prevalence of diabetes. Further study is needed to verify the causality of this association and elucidate the underlying mechanisms.
Genomic analysis and biochemical profiling of an unaxenic strain of Synechococcus sp. isolated from the Peruvian Amazon Basin region
Authors: Cobos Marianela , Condori Ruth C. , Grandez Miguel A. , Estela Segundo L. , Del Aguila Marjorie T. , Castro Carlos G. , Rodríguez Hicler N. , Vargas Jhon A. , Tresierra Alvaro B. , Barriga Luis A. , Marapara Jorge L. , Adrianzén Pedro M. , Ruiz Roger , Castro Juan C. 
Published: November 2022

Cyanobacteria are diverse photosynthetic microorganisms able to produce a myriad of bioactive chemicals. To make possible the rational exploitation of these microorganisms, it is fundamental to know their metabolic capabilities and to have genomic resources. In this context, the main objective of this research was to determine the genome features and the biochemical profile of Synechococcus sp. UCP002. The cyanobacterium was isolated from the Peruvian Amazon Basin region and cultured in BG-11 medium. Growth parameters, genome features, and the biochemical profile of the cyanobacterium were determined using standardized methods. Synechococcus sp. UCP002 had a specific growth rate of 0.086 ± 0.008 μ and a doubling time of 8.08 ± 0.78 h. The complete genome of Synechococcus sp. UCP002 had a size of ∼3.53 Mb with a high coverage (∼200x), and its quality parameters were acceptable (completeness = 99.29%, complete and single-copy genes = 97.5%, and contamination = 0.35%). Additionally, the cyanobacterium had six plasmids ranging from 24 to 200 kbp. The annotated genome revealed ∼3,422 genes, ∼ 3,374 protein-coding genes (with ∼41.31% hypothetical protein-coding genes), two CRISPR Cas systems, and 61 non-coding RNAs. Both the genome and plasmids had the genes for prokaryotic defense systems. Additionally, the genome had genes coding the transcription factors of the metalloregulator ArsR/SmtB family, involved in sensing heavy metal pollution. The biochemical profile showed primary nutrients, essential amino acids, some essential fatty acids, pigments (e.g., all-trans-β-carotene, chlorophyll a, and phycocyanin), and phenolic compounds. In conclusion, Synechococcus sp. UCP002 shows biotechnological potential to produce human and animal nutrients and raw materials for biofuels and could be a new source of genes for synthetic biological applications.
Oculomotor impairments in de novo Parkinson’s disease
Authors: Zhou Meng-Xi , Wang Qin , Lin Yin , Xu Qian , Wu Li , Chen Ya-Jing , Jiang Yu-Han , He Qing , Zhao Lei , Dong You-Rong , Liu Jian-Ren , Chen Wei 
Published: November 2022

ObjectiveReliable electrophysiological indicators are urgently needed in the precise evaluation of Parkinson’s disease (PD). It is still elusive whether oculomotor performance is impaired or has clinical value in early PD. This study aims to explore oculomotor performance in newly diagnosed, drug-naïve PD and its correlation with clinical phenotype.MethodsSeventy-five patients with de novo PD, 75 patients with essential tremor (ET), and 46 gender-and age-matched healthy controls (HCs) were included in this cross-sectional study. All subjects underwent oculomotor test via videonystagmography. Visually guided saccade latency, saccadic accuracy and gain in smooth pursuit eye movement (SPEM) at three frequencies of the horizontal axis were compared among the three groups. Patients with PD also received detailed motor and non-motor evaluation by serial scales. The association between key oculomotor parameters and clinical phenotypes were explored in PD patients.ResultsBoth de novo PD and ET patients showed prolonged saccadic latency and decreased saccadic accuracy relative to HCs. SPEM gain in PD was uniformly reduced at each frequency. SPEM gain at 0.4 Hz was also decreased in ET compared with HCs. However, there was no significant difference of oculomotor parameters between de novo PD and ET patients. Furthermore, prolonged saccadic latency was correlated with long disease duration, whereas decreased SPEM gain was associated with severe motor symptoms in de novo PD patients.ConclusionOcular movements are impaired in de novo, drug naïve PD patients; these changes could be indicators for disease progression in PD.
Case report: Concurrent intravestibular schwannoma mimicking Ménière's disease and cochlear hydrops detected via delayed three-dimensional fluid-attenuated inversion recovery magnetic resonance imaging
Authors: Nishimura Koji , Murofushi Toshihisa , Hakuba Nobuhiro 
Published: November 2022

ObjectiveTo present a case of intralabyrinthine schwannoma (ILS) presenting as Ménière's disease diagnosed via 4-h delayed gadolinium-enhanced three-dimensional fluid-attenuated inversion recovery magnetic resonance imaging (3D-FLAIR MRI) and treated successfully using the translabyrinthine approach.PatientA patient who was diagnosed with intravestibular ILS.InterventionsThe patient underwent comprehensive preoperative neurological examinations and MRI. The tumor was resected using the translabyrinthine approach and was pathologically confirmed as schwannoma based on the surgical specimen.Main outcome measuresPreoperative audiogram and vestibular test findings and MRI images.ResultsPreoperatively, pure-tone audiogram showed progressive sensorineural hearing loss only on the affected side. The video head impulse test and vestibular evoked myogenic potential test showed vestibular dysfunction on the affected ear. Immediate gadolinium-enhanced T1-weighted MRI revealed an enhanced region in the vestibule. Meanwhile, magnetic resonance cisternography showed a filling defect. Delayed 3D-FLAIR MRI revealed a signal void in the scala media of the cochlea indicative of cochlear hydrops, and a strong signal in the perilymph at the basal cochlea suggestive of impaired blood–labyrinthine barrier.ConclusionDelayed 3D-FLAIR MRI is useful in diagnosing concurrent ILSs and endolymphatic hydrops.
Red cell distribution width is associated with stroke severity and unfavorable functional outcomes in ischemic stroke
Authors: Xue Jie , Zhang Dong , Zhang Xiao-Guang , Zhu Xiao-Qiong , Xu Xu-Shen , Yue Yun-hua 
Published: November 2022

BackgroundRed blood cell distribution width (RDW) is considered to be related to coronary heart disease and heart failure and all-cause mortality, but its relationship with acute ischemic stroke is still unclear. In this study, we aimed to explore the relationship between RDW and the stroke severity and functional outcomes of ischemic stroke.MethodsWe retrospectively reviewed patients with acute ischemic stroke between September 2016 and January 2020. Demographic, clinical, stroke complications, laboratory data, and treatment were collected for all patients. Stroke severity and functional outcomes were evaluated by NIHSS score, modified Rankin Scale (mRS), and Barthel Index (BI) at 3 months. Furthermore, multiple logistic regression analysis was used to assess the relationship between RDW and stroke severity and functional outcomes.ResultsA total of 629 patients with acute ischemic stroke were included and were categorized into four groups according to the quartiles of RDW (&lt; 12.4, 12.4–12.9, 13.0–13.4, &gt; 13.4). After multivariable analysis, higher RDW was directly associated with moderate to severe stroke (OR 2.21, 95% CI, 1.30–3.75, P = 0.003), mRS score of 3–6 at 3 months (OR 1.86, 95% CI, 1.02–3.41, P = 0.044), and BI score below 85 at 3 months (OR 2.27, 95% CI, 1.25–4.12, P = 0.007) in patients with ischemic stroke.ConclusionOur results demonstrate that RDW is associated with stroke severity and unfavorable functional outcomes at 3 months in patients with ischemic stroke.
Integrated bioinformatics analysis and experimental validation reveals fatty acid metabolism-related prognostic signature and immune responses for uterine corpus endometrial carcinoma
Authors: Guo Chenrui , He Yan , Chen Leiming , Li Yuan , Wang Yajun , Bao Yunlei , Zeng Ni , Jiang Feng , Zhou Hang , Zhang Le 
Published: November 2022

BackgroundUterine corpus endometrial carcinoma (UCEC) is the third most common gynecologic malignancy. Fatty acid metabolism (FAM) is an essential metabolic process in the immune microenvironment that occurs reprogramming in the presence of tumor signaling and nutrient competition. This study aimed to identify the fatty acid metabolism-related genes (FAMGs) to develop a risk signature for predicting UCEC.MethodsThe differentially expressed FAMGs between UCEC samples and controls from TCGA database were discovered. A prognostic signature was then constructed by univariate, least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression analyses. Based on the median risk score, UCEC samples were categorized into high- and low-FAMGs groups. Kaplan-Meier (K-M) curve was applied to determine patients’ overall survival (OS). The independent prognostic value was assessed by uni- and multivariate analyses. The associations between the risk score and immune status, immune score, and drug resistance were evaluated. Quantitative Real-time PCR (qRT-PCR) was utilized to confirm FAMGs expression levels in UCEC cells.ResultsWe built a 10-FAMGs prognostic signature and examined the gene mutation and copy number variations (CNV). Patients with a high-FAMGs had a worse prognosis compared to low-FAMGs patients in TCGA train and test sets. We demonstrated that FAMGs-based risk signature was a significant independent prognostic predictor of UCEC. A nomogram was also created incorporating this risk model and clinicopathological features, with high prognostic performance for UCEC. The immune status of each group was varied, and immune score was higher in a low-FAMGs group. HLA-related genes such as DRB1, DMA, DMB, and DQB2 had higher expression levels in the low-FAMGs group. Meanwhile, high-FAMGs patients were likely to response more strongly to the targeted drugs Bortezomib, Foretinib and Gefitinib. The qRT-PCR evidence further verified the significant expression of FAMGs in this signature.ConclusionsA FAMGs-based risk signature might be considered as an independent prognostic indicator to predict UCEC prognosis, evaluate immune status and provide a new direction for therapeutic strategies.
Circulating miR-326 could serve as a predictive biomarker for response to neoadjuvant chemotherapy in locally advanced cervical cancer
Authors: Zou Kangni , Yang E. , Cui Tao , Li Zhengyu 
Published: November 2022

BackgroundClinically, few patients with locally advanced cervical cancer (LACC) are insensitive to neoadjuvant chemotherapy (NACT). Recent studies have reported that circulating microRNAs (miRNAs) may be involved in the response to NACT. The aim of this study was to discover the potential miRNAs that can predict the response to NACT in LACC.MethodsPair-matched blood samples of 39 LACC patients before and after receiving NACT were collected. Seven paired samples were used for microRNA microarray analysis. Targeted miRNAs were selected by bioinformatics analysis and were validated by quantitative reverse transcription–polymerase chain reaction (qRT-PCR). All 39 patients were assigned into either the responders group or the non-responders group after NACT. The predictive performance of selected microRNA was evaluated by sensitivity, specificity, accuracy, and the area under the receiver operating characteristic (ROC) curve.ResultsA total of 17 miRNAs downregulated before NACT and upregulated after NACT were selected according to microarray analysis in our previous study, and miR-326 and miR-376a-3p were selected for further exploration. According to the responses and the evaluation criteria, 25 patients reached partial response (PR) and 14 patients remained stable. Further qRT-PCR analysis showed that miR-326 significantly downregulated before NACT and upregulated after NACT in 12 responders (p = 0.02). The expression of miR-376a-3p showed no statistical difference before and after NACT in these 12 responders. Then, miR-326 provided an AUC-ROC of 0.75 (p = 0.04) in the discrimination between the responders and non-responders groups. The cutoff value of ROC for miR-326 to predict the response of NACT was &lt;0.023, the sensitivity was 88.89%, and the specificity was 50%.ConclusionsThe expression of miR-326 significantly upregulated after NACT in responders. miR-326 may be a biomarker for predicting the response to NACT in LACC patients. The results may optimize individualized treatments for LACC patients.