bluuebunny/crossref_metadata_2025_split huggingface dataset:
Personalized health risk assessment based on single-cell RNA sequencing analysis of a male with 45, X/48, XYYY karyotype
Authors: Koczkowska Magdalena , Jąkalski Marcin , Birkholz-Walerzak Dorota , Kostecka Anna , Iliszko Mariola , Wójcik Magdalena , Lewandowski Krzysztof , Milska-Musa Katarzyna , Buckley Patrick G. , Drężek Kinga , Juhas Ulana , Kuziemska Ewa , Maciejewska Agnieszka , Pawłowski Ryszard , Wasąg Bartosz , Filipowicz Natalia , Chojnowska Katarzyna , Ławrynowicz Urszula , Dumanski Jan P. , Lipska-Ziętkiewicz Beata S. , Mieczkowski Jakub , Piotrowski Arkadiusz 
Published: December 2022

AbstractNumeric sex chromosome abnormalities are commonly associated with an increased cancer risk. Here, we report a 14-year-old boy with a rare mosaic 45, X/48, XYYY karyotype presenting with subtle dysmorphic features and relative height deficiency, requiring growth hormone therapy. As only 12 postnatal cases have been described so far with very limited follow-up data, to assess the proband’s long-term prognosis, including cancer risk, we performed high-throughput single-cell RNA sequencing (scRNA-seq) analysis. Although comprehensive cytogenetic analysis showed seemingly near perfect balance between 45, X and 48, XYYY cell populations, scRNA-seq revealed widespread differences in genotype distribution among immune cell fractions, specifically in monocytes, B- and T-cells. These results were confirmed at DNA level by digital-droplet PCR on flow-sorted immune cell types. Furthermore, deregulation of predominantly autosomal genes was observed, including TCL1A overexpression in 45, X B-lymphocytes and other known genes associated with hematological malignancies. Together with the standard hematological results, showing increased fractions of monocytes and CD4+/CD8+T lymphocytes ratio, long-term personalized hemato-oncological surveillance was recommended in the reported patient.
Piezoelectric conductive electrospun nanocomposite PCL/Polyaniline/Barium Titanate scaffold for tissue engineering applications
Authors: Peidavosi Naeemeh , Azami Mahmoud , Beheshtizadeh Nima , Ramazani Saadatabadi Ahmad 
Published: December 2022

AbstractRecent trends in tissue engineering technology have switched to electrical potentials generated through bioactive scaffolds regarding their appropriate effects on cell behaviors. Preparing a piezo-electrical stimuli scaffold with high electrical conductivity for bone and cartilage tissue regeneration is the ultimate goal of the present study. Here, Barium Titanate nanoparticles (BaTiO3 NPs) were used as piezoelectric material and highly conductive binary doped Polyaniline nanoparticles (PANI NPs) were synthesized by oxidative polymerization. Polycaprolactone (PCL) was applied as carrier substrate polymer and conductive spun nanofibrous scaffolds of PCL/PANI composites were prepared in two different amounts of PANI (3 and 5 wt.%). The conductivity of PCL/PANI nanofibers has been analyzed by standard four probes test. Based on the obtained results, the PCL/PANI5 (with 5 wt.% PANI) was selected due to the superior electrical conductivity of 8.06 × 10–4 s cm - 1. Moreover, the piezoelectric nanofibrous scaffolds of PCL/BT composite were electrospun in three different amounts of BT (20, 30, and 40 wt.%). To investigate the synergic effect of conductive PANI and piezoelectric BT, ternary nanocomposite scaffolds of PCL/PANI/BT were prepared using the dual jet electrospinning technique. The piezoelectric properties have been analyzed by determining the produced voltage. The morphological assessment, contact angle, mechanical test, and MTT assay have been conducted to evaluate other properties including biocompatibility of nanofibrous scaffolds. The PCL/PANI5/BT40 composite resulted in an unprecedented voltage of 1.9 Volt. SEM results confirm that BT NPs have been distributed and embedded inside PCL fibers quite appropriately. Also, the chosen scaffolds were homogeneously intertwined and possessed an average fiber diameter of 288 ± 180 nm, and a contact angle of 92 ± 7°, making it a desirable surface for cell attachment and protein interactions. Moreover, Young’s modulus, ultimate tensile stress, and elongation were obtained as 11 ± 1 MPa, 5 ± 0.6 MPa, and 109 ± 15% respectively. Obtained results assert the novel potential of piezo-electrical stimuli conductive nanocomposite scaffold for tissue engineering applications.
Cofactors, age at onset, allergic comorbidities and gender are different in patients sensitized to omega-5 gliadin and Pru p 3
Authors: Costanzo Giulia , Matolo Annalisa , Saderi Laura , Messina Maria Rita , Firinu Davide , Barca Maria Pina , Serra Paolo , Corso Nicoletta , Sotgiu Giovanni , Del Giacco Stefano 
Published: December 2022

AbstractAim of this study is to clarify the impact of cofactors on allergic reactions in patients sensitized to LTP and ω-5-gliadin. We retrospectively examined the charts of our outpatients from January 2015 to July 2019 and identified 157 patients seen for urticaria/angioedema or anaphylaxis after a meal, in presence or absence of cofactors and sensitized to LTPs (Pru p 3 and/or Tri a 14) and/or ω-5-gliadin (Tri a 19). we compared LTPs-positive patients and those sensitized to Tri a 19 in order to assess the difference in cofactors involved and in frequency of clinical presentation. Our data show that exercise is the most frequent cofactor in FDEIAn and the involvement of exercise, alcohol and multiple cofactors was more frequently found in males than in females. We found that exercise, pollen counts peaks and multiple cofactors were more often related to anaphylaxis than mild reactions. Finally, we performed a comparison between patients LTPs+ and Tri a 19+ that showed in the latter group a lower frequency of allergic comorbidities, a higher median age at the onset of symptoms and frequency of alcohol exposure. Our data show that the search for possible cofactors involved in food allergy is essential not only for diagnostic purposes, but also for risk assessment strategies.
No survival benefit from adding chemotherapy to adjuvant radiation in advanced major salivary gland cancer
Authors: Kang Nai-Wen , Kuo Yu-Hsuan , Wu Hung-Chang , Ho Chung-Han , Chen Yi-Chen , Yang Ching-Chieh 
Published: December 2022

AbstractThis study aimed to compare survival of patients with advanced major salivary gland cancers treated with adjuvant chemoradiation therapy (CRT) versus radiotherapy (RT) alone, after surgical resection. The Taiwan Cancer Registry database was used to identify patients (2009–2017) with advanced (T3–4 or nodal positivity) major salivary gland cancers, treated post-surgically with adjuvant CRT or RT alone. Overall survival (OS) and disease-specific survival (DSS) evaluated using Kaplan–Meier. Stratified analyses conducted on clinicopathological features. A total of 395 patients were analyzed: 178 (45.1%) received adjuvant CRT; 217 (54.9%) received adjuvant RT alone. Median radiation dose was 66 Gy in 33 fractions. Cisplatin was most common chemotherapy regimen. After a median follow-up of 3.37 years, there was no significant difference in OS or DSS (p = 0.1354 and 0.3361, respectively) between groups. Adding chemotherapy to adjuvant RT was not significantly associated with improved OS (adjusted hazard ratio [aHR] 0.94; 95% CI 0.72–1.23) and DSS (aHR 0.96; 95% CI 0.72–1.28). Stratified analysis of clinicopathological features found no significant advantages for improved OS or DSS from adding chemotherapy to adjuvant RT. Thus, in this population database, the use of chemotherapy provided limited survival benefits in advanced major salivary gland cancers after surgical resection.
The association between Acinetobacter baumannii infections and the COVID-19 pandemic in an intensive care unit
Authors: Boral Jale , Genç Zeliha , Pınarlık Fatihan , Ekinci Güz , Kuskucu Mert A. , İrkören Pelin , Kapmaz Mahir , Tekin Süda , Çakar Nahit , Şentürk Evren , Yurdakul Fatma , Dikenelli Bilge , Can Fusun , Ergonul Onder 
Published: December 2022

AbstractWe aimed to describe the increased rate of Acinetobacter baumannii infections during the COVID-19 pandemic and define its significance within the last five years. This study was performed in a tertiary hospital with 280 beds and included all patients infected with A. baumannii in the intensive care unit between January 1, 2018, and June 30, 2022. A. baumannii-infected patients in the intensive care unit 27 months before the pandemic and 27 months during the pandemic were included. Pulsed-field gel electrophoresis was performed to assess clonal relatedness. The infection control measures were specified based on the findings and targeted elimination. In total, 5718 patients were admitted to the intensive care unit from January 1st, 2018, to June 30th, 2022. A. baumannii infection was detected in 81 patients. Compared to the pre-pandemic era, the rate of A. baumannii infection during the pandemic was 1.90 times higher (OR: 1.90, 95% CI: [1.197, 3.033]). Clonality assessment of multidrug-resistant A. baumannii samples revealed eight clusters with one main cluster comprising 14/27 isolates between 2021 and 2022. The case fatality rate of the pre-pandemic and pandemic era was not different statistically (83.33% vs. 81.48%, p = 0.835). Univariate analysis revealed the association of mechanical ventilation (p = 0.002) and bacterial growth in tracheal aspirate (p = 0.001) with fatality. During the COVID-19 pandemic, potential deficits in infection control measures may lead to persistent nosocomial outbreaks. In this study, the introduction of enhanced and customized infection control measures has resulted in the containment of an A. baumannii outbreak.
Mitigating the impact of fast charging on distribution grids using vehicle‐to‐vehicle power transfer: A Paris city case study
Authors: Sehimi Yacine , Almaksour Khaled , Suomalainen Emilia , Robyns Benoit 
Published: December 2022

AbstractThe deployment of DC fast charging stations (DCFCSs) in cities requires costly reinforcement of low‐voltage (LV) distribution grids, due to the high power required. It results in high investments for the station operator as well as for the distribution system operator and limits the profitability of such projects. In this study, a novel concept of fast charging station which reduces the connection to the grid is presented. This station uses Vehicle‐to‐vehicle (V2V) power transfer from a fleet of carsharing electric vehicles to provide a high charging power while mitigating the power demand on the grid. Based on data from a carsharing operator in the city of Paris, a simulation model of a combined carsharing/V2V fast charging system is proposed, allowing to simulate carsharing trips and V2V transfers. The reduction of the grid connections required to provide fast charging is evaluated and the impact of the repeated discharges on the level of service of the carsharing system is discussed. Moreover, simulation of LV distribution grids allows to compare the grid losses of the V2V system with conventional DCFCSs.
Evaluation of the impact of the electric network's quality on the appliance's signatures
Authors: Sonfack Gisele Beatrice , Ravier Philippe 
Published: December 2022

AbstractThe continuous increase in the demand for electrical energy has led to the design of highly complex electrical systems, which can even extend to entire countries. In this context, active residential customer participation in the smart grid can benefit from having control over a number of data points related to the behaviour of the grid and the devices connected to it. The load signatures are the basis for practical energy's consumption monitoring technologies, which involve identifying an electrical device and determining their operating status. Although several load identification techniques are under development in research laboratories, it is important to note that studies on the impact of power system quality on the signatures underlying these developments are not yet clear. Knowing that load profiles depend on environmental factors, user habits, and other non‐deterministic factors, it is important to assess the impact that grid quality can have on load signatures in order to take it into account in the development of identification systems to be integrated into the smart grid. This research focuses on the two central components of these approaches: (1) Presenting an assessment of the different network faults that can impact the signatures of connected loads, (2) Analysing and quantifying the impact of each fault through load behavioural models and virtual simulation. The study considers the two types of loads generally encountered in homes (linear and non‐linear). It then analyses the characteristic quantities such as waveforms, effective current and voltage values, active and reactive power (harmonics) and non‐deforming power (fundamentals). The results of the simulations performed on Simulink/Simscape show that some network faults such as flicker have a significant and permanent impact on the signatures while others (Voltage Sag) have a temporary and sometimes insignificant effect.
Update FDG-PET in der Demenzdiagnostik
Authors: Buchert Ralph , Förster Stefan 
Published: December 2022

ZusammenfassungDieser Artikel soll ein Update zu unserem Übersichtsartikel „FDG-PET in der
Differenzialdiagnostik neurodegenerativer Demenzerkrankungen“ aus 2016 geben. Seitdem wurden
zahlreiche neue, technisch hochwertige Studien mit großen Patientenkollektiven sowie
systematische Übersichtsarbeiten internationaler Expertengruppen veröffentlicht. Außer den
aktualisierten Best-Practice-Empfehlungen dieser Expertengruppen sollen in diesem Update
einige ausgewählte neue Entwicklungen vorgestellt und diskutiert werden, die aus unserer Sicht
für den Einsatz der FDG-PET des Gehirns in der klinischen Routineversorgung von Patienten mit
kognitiven Einschränkungen besonders relevant sind, oder in naher Zukunft besonders relevant
werden könnten. Dazu gehören neue diagnostische Optionen durch die verbesserte räumliche
Auflösung der klinischen Hirn-PET mit „extraschnellen“ Time-of-Flight
Ganzkörper-PET/CT-Systemen und der Einsatz Künstlicher Intelligenz zur automatischen
Klassifikation der FDG-PET des Gehirns basierend auf konventionellen Kovarianzanalysen oder
Deep Learning mit künstlichen neuronalen Netzen.
Trial of Labor after Three or More Previous Cesarean Sections:
Systematic Review and Meta-Analysis of Observational Studies
Authors: Fruscalzo Arrigo , Rossetti Emma , Londero Ambrogio P. 
Published: December 2022

Abstract
Aims To assess the success rate and prevalence of maternal or neonatal
complications in women undergoing a trial of labor after three or more
(≥3) previous cesarean sections (CSs).
Methods A systematic literature review and meta-analysis was conducted
from inception to May 2022 in Medline, Scopus, ENBASE, ClinicalTrials.gov, and
the Cochrane Central Register of Controlled Trials and Reviews. Items detailing
success rate and complications in women with a history of≥3 previous CSs
were considered. Selected articles were evaluated for quality, heterogeneity,
and publication bias. A pooled prevalence or odds ratio was calculated.
Findings Twelve articles were included for a total of 540 women with a
history of≥3 CSs, accounting for the 2% (CI 95%
1–4%) of the whole cohort of trial of labor. Our findings show a
0.67 (CI 95% 0.53–0.78) rate of successful vaginal delivery. A
higher success rate was observed in women having a history of a prior vaginal
delivery (0.90, CI 95% 0.77–0.96) and when prostaglandins,
peridural anesthesia or oxytocin were allowed (respectively 0.73, CI 95%
0.62–0.83, 0,73, CI 95% 0.57–0.85 and 0.73, CI
95% 0.64–0.81). Uterine rupture rate was 0.01 (CI 95%
0.00–0.01). No cases of fetal asphyxia or maternal or neonatal death
were registered.
Conclusions The success rate and low frequency of severe complications
observed seem to support a trial of labor in selected patients desiring a
natural birth. However, a potential underestimation of serious maternal and
neonatal complications should be considered in the decision-making process.
A Cytogenetic Study of Turkish Children with Global Developmental Delay
Authors: Demirhan Osman , Hergüner Özlem , Tunç Erdal 
Published: December 2022

AbstractGlobal developmental delay (GDD)/intellectual disability (ID) is common in children and its etiology is unknown in many cases. Chromosomal abnormalities are predominant genetic causes of GDD/ID. The aim of this study is to determine the genetic risk factors that may be involved in the etiology of GDD/ID. In this study, 810 children with moderate to severe, clinically unexplained GDD/ID for whom cytogenetic analysis were performed were retrospectively rescreened. The results showed that GDD/ID affected more females than males (2 girls:1 boy). A total of 54 children (6.7%) with GDD showed chromosomal aberrations (CAs): 59.3% of these CAs were structural aberrations, and the rest were numerical aberrations (40.7%). Specifically, inversions, deletions, and reciprocal and robertsonian translocations, which were detected in 1, 0.7, 0.8, and 0.4% of the children, respectively, constituted important categories of structural CAs. Among numerical CAs, classic Turner and mosaics were detected in 1.2% of all children. Trisomy 21 and mosaic trisomy 21 were detected in 1% of the children. Marker chromosomes and 47,XXY karyotypes were found in two children each. Our results suggest that female sex is more affected by CAs among GDD/ID cases, and cytogenetic analysis is useful in the etiological diagnosis of GDD/ID.
Iatrogenic Withdrawal Syndrome in Critically Ill Neonates: A Review of Mechanisms, Assessment, Management, and Prevention
Authors: dos Santos Isaque Nilton , Peano Carolina Teixeira Goulart , Soares Ericks Sousa , Cimarosti Helena Iturvides 
Published: December 2022

AbstractConsidering that neonatal pain is a relevant matter given its implications and consequences, many neonates, particularly those undergoing critical care, are subjected to analgo-sedation therapies, which commonly includes treatment with opioids and benzodiazepines. These drugs, however, can induce tolerance and dependency, leading to the development of the so-called iatrogenic withdrawal syndrome (IWS) which is observed following discontinuation of these therapeutic agents particularly when utilized for a prolonged period of time (≥5 days). IWS consists of signs and symptoms manifested especially in young children, such as term and premature newborns in the neonatal intensive care unit, who are less capable of metabolizing and eliminating these drugs, compared with older patients. In this study, we review assessment tools that were developed to identify, evaluate, and manage children affected by IWS. The studies reviewed demonstrate that optimal management of IWS includes consideration of alternate routes of drug administration, the need for adequate time for drug tapering, and also the presence of planned rescue therapy when encountering cases refractory to ongoing management. Equally important is prevention of IWS which can be accomplished with the implementation of drug rotation protocols and, adherence to evidenced-based guidelines which facilitate an overall decline in the use and duration of opioids and benzodiazepines. Finally, our review strongly supports the need for more research on IWS in neonates given their increased susceptibility and sparse published data for this age group.
Investigation of the Antimicrobial Effect of Different Disinfectants on Alginate Measurement Materials
Authors: Güntekin Neslihan , Arslan Gökçe Kader , Özdemir Mehmet , Tunçdemir Ali Rıza 
Published: December 2022

Abstract
Objective The impression materials are contaminated with pathogenic microorganisms carried by saliva and blood in the mouth. The aim of this study is to evaluate the antimicrobial activity of 1 and 5.25% sodium hypochlorite (NaClO) and zeta 7 solution, a commercial disinfectant, on alginate and to compare it washing with distilled water.
Methods Samples obtained from alginate impressions taken from eight pediatric patients selected in accordance with the inclusion criteria with a size of 1 × 1 cm were subjected to five different disinfection protocols. After samples were vortexed in 3-mL physiological saline, 1-mL volume were inoculated onto brain heart infusion agar and cultured at 37°C for 72 hours to determine the number of colony-forming units/mL. Streptococcus mutans, Staphylococcus aureus, Escherichia coli, Candida albicans were used as control strains. The data obtained were statistically evaluated by using analysis of variance and post-hoc tests. The differences were considered statistically significant when p < 0.05.
Results All of the disinfectant solutions tested were effective in the disinfection of the impression materials examined. The control group with no treatment and the water and flushing group had a significantly higher bacterial load compared to the other disinfectant-treated groups in terms of bacterial load, and no significant difference has been observed between the disinfectant solutions (p < 0.05). Simple rinsing of the impressions in sterile water reduced the number of microorganisms but did not decontaminate the impressions.
Conclusion Washing with water alone is insufficient for measured disinfection. Although NaClO is an effective disinfectant even at low concentrations, microorganism growth was not observed at 5.25% concentration. It is appropriate to use NaClO at 1 and 5.25% concentrations for impression disinfection.
A simple system for measuring the transmittance curve of x-ray filters at low x-ray energies
Authors: Cao Jiewei , Chen Tianxiang , He Huilin , Xu Yupeng , Cui Xingzhu , Gao Na , Xu Xiongwei 
Published: December 2022

To prevent the x-ray detectors from the interference of optical photons, x-ray filters are widely used in x-ray space missions. As the flux of low energy x-rays would be significantly attenuated by the filters, it is important to derive the transmittance of the filters below 10 keV. In this paper, we present a simple experimental apparatus and data processing method to measure the transmittance curve of x-ray filters in the energy range from 1.6 to 10 keV. The measured spectra were further processed with Geant4 Monte Carlo simulation results and a direct demodulation algorithm. The obtained transmittance is in good accordance with the theoretical calculation and the synchrotron radiation test result, which proves that the proposed system with the algorithm is a reasonable way to characterize the x-ray transmittance performance of filters above 1.6 keV.
Elastocaloric effect characterization of a NiTi tube to be applied in a compressive cooler
Authors: Cheng Siyuan , Xiao Yinan , Li Xueshi , Lin Hongyang , Hua Peng , Sheng Liyuan , Sun Qingping 
Published: December 2022

In this article, the elastocaloric effect of a commercial superelastic NiTi shape memory alloy (SMA) tube (with an outer diameter of 5 mm and wall thickness of 1 mm) to be applied in a compressive cooler was measured and analyzed. The elastocaloric effect of the tube was measured vs the applied strain and strain rate. The largest temperature changes of 21 K during loading and 16 K during unloading were measured at an applied strain of 3.30% and strain rate of 0.33 s−1. In the fatigue testing of the sample, only 0.20% of the residual strain accumulated after a runout of 1 × 106 sinusoidal force-controlled loading–unloading cycles at a maximum compressive stress of 1100 MPa and frequency of 20 Hz. Numerical results of the cooling characteristics of a compressive device using a single NiTi tube with the above-mentioned cross section and an aspect ratio of 60:1 as the refrigerant showed that the device could produce a total cooling power of up to 20 W and a coefficient of performance of up to 6.5. The results of this article demonstrate that superelastic NiTi SMA tubes of suitable wall thickness and aspect ratios are good candidates to be applied in a compressive elastocaloric cooler.