bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age-related effects of fenofibrate on the hepatic expression of sirtuin 1, sirtuin 3, and lipid metabolism-related genes
Authors: Zubrzycki Adrian , Wronska Agata , Wierzbicki Piotr M. , Kmieć Zbigniew 
Published: August 2022

Abstract
Background: Sirtuin 1 (Sirt1) and sirtuin 3 (Sirt3) participate in regulation of lipid metabolism. Our aim was to investigate the effects of the hypolipemic drug fenofibrate (FN) on hepatic Sirt1 and Sirt3 expression, in relation to the expression of lipid metabolism-related genes and in the context of aging. Methods and Results: Young and old male Wistar rats were fed standard chow or supplemented with 0.1% or 0.5% FN for 30 days (n = 7-10 in each group). In young rats, 0.1% FN did not affect Sirt1 expression, however, 0.5% FN decreased Sirt1 and both doses reduced Sirt3 protein levels. In old rats, 0.5% FN decreased hepatic Sirt1 mRNA and both doses reduced Sirt1 protein levels, but not Sirt3 expression. Although hepatic Pparα protein levels did not change, FN treatment of young rats induced Cpt1b expression, whereas Lcad, Acox1, Pmp70, and Hmgcs2 expression increased only after 0.1% FN, and Fas2 expression decreased after 0.5% FN. In the liver of old rats, both doses increased Cpt1b and Lcad expression. Only 0.1% FN increased Pmp70 and Hmgcs2 expression, and only 0.5% FN increased Acox1 and Fas2 mRNA levels. Conclusions: Treatment with fenofibrate at moderate or high dose may downregulate the hepatic expression of Sirt1 and Sirt3 proteins. The dosage of FN affects the molecular changes, and aging alters the response to 0.5% FN.
SDSS ameliorates cerebral ischemia/reperfusion injury by inhibiting CLIC4/NLRP3 inflammasome-mediated endothelial cell pyroptosis
Authors: Zhang Xiaolu , Yang Qiuyue , Guo Lin , Zhang Yilin , Zeng Wenyun , Yu Qun , Zeng Miao , Gan Jiali , Li Huhu , Yang Lin , Jiang Xijuan , Gao Qing 
Published: August 2022

Abstract
Background: Endothelial pyroptosis promotes cerebral ischemic-reperfusion injury (CIRI). Sodium Danshensu (SDSS) has been shown to attenuate CIRI and have anti-inflammatory properties in endothelial cells. Nevertheless, the mechanism of SDSS on endothelial pyroptosis after CIRI remains unclear. Objective: We aimed to investigate the efficacy and mechanism of SDSS for reducing endothelial pyroptosis. Methods: In vitro, the effect of SDSS alleviating CIRI was first confirmed by detecting pyroptosis and NLRP3 inflammasome related indicators in oxygen-glucose deprivation/reoxygen (OGD/R) treated bEnd3 cells. Further, CLIC4 was identified as a potential target of SDSS through protein microarray, molecular docking, and cellular thermal shift assay (CETSA). Following this, the translocation and expression of CLIC4, and chloride outflow were detected. Finally, CLIC4 was further tested, either overexpressed or knocked down, to determine whether it is a target of SDSS to inhibit endothelial pyroptosis. In vivo, neurological deficit scores and infarct volume were served to evaluate the effect of SDSS in middle cerebral artery occlusion/reperfusion (MCAO/R) rats. Further investigation of pyroptosis was conducted using the CLIC4/NLRP3/GSDMD pathway. Results: SDSS administration inhibited NLRP3 inflammasome mediated pyroptosis in vitro and vivo. As demonstrated by protein microarray, molecular docking and CETSA, SDSS bound strongly to CLIC4 and decreased its protein level, and inhibited its translocation from cytoplasm to cell membrane. Further, SDSS effectiveness was weakened by CLIC4 overexpression but not knockdown. Conclusion: The present study indicated that a beneficial effect of SDSS against CIRI was ascribed to block endothelial pyroptosis via binding to CLIC4, and then inhibiting chloride efflux-dependent NLRP3 inflammasome activation.
Optimal method for detecting cervical lymph node metastasis from papillary thyroid cancer
Authors: Xiao Jing , Meng Shuyu , Zhang Mingbo , Li Yingying , Yan Lin , Li Xinyang , Yang Zhen , Zhang Yan , Luo Yukun 
Published: August 2022

Abstract

Purpose Papillary thyroid cancer (PTC) is often accompanied by cervical lymph node metastases (LNM). This study compared the diagnostic performance of fine needle aspiration (FNA), the combination of FNA and thyroglobulin measurement in the washout (FNA-Tg), and core needle biopsy(CNB) in order to determine the optimal method in detecting cervical LNM from PTC. Methods PTC patients with suspicious cervical lymph nodes, who underwent ultrasonography-guided CNB, or FNA were retrospectively reviewed between January 2021 and April 2022. Patients’ demographics, lymph node size, and results of FNA, FNA-Tg, CNB and surgical pathology were collected. Sensitivitity, specificity, positive predictive value (PPV), negative predictive value (NPV), accuracy and the area under the curves (AUC) of receiver operating characteristic were obtained. Results FNA-Tg had a higher AUC with a threshold of 55 ng/mL than that with a threshold of 1 ng/mL (0.782 vs. 0.636,P = 0.005). The sensitivity of the combination of FNA and FNA-Tg was higher than that of FNA alone (91.3% vs. 81.0%,P = 0.01), but it was not different from that of CNB (91.3% vs. 88.0%,P > 0.05). No significant difference was found in specificity, PPV, NPV, accuracy and AUC among FNA alone, CNB alone, and the combination of FNA and FNA-Tg. Conclusions FNA-Tg is useful to improve the sensitivity of FNA. The diagnostic performance of the combination of FNA and FNA-Tg is better than that of FNA or FNA-Tg alone, but is not different from that of CNB in detecting LNM from PTC.
Comparison of tramadol and lornoxicam for the prevention of postoperative catheter-related bladder discomfort: A randomized controlled trial
Authors: Liao Xin , Xie Min , Li Shuying , Yu Xiaolan 
Published: August 2022

Abstract
Background
Catheter-related bladder discomfort (CRBD) is a painful complication of intraoperative urinary catheterization after anesthesia. We conducted this study to compare the effect of tramadol and lornoxicam for the prevention of postoperative CRBD.
Methods
One hundred twenty patients (aged 18–60 years), ASA physical status I-II, undergoing elective uterine surgery requiring intraoperative urinary catheterization were randomly divided into three groups with 40 patients in each group. Group T received 1.5 mg/kg tramadol, Group L received 8 mg lornoxicam, and Group C received normal saline. The study drugs were administered intravenously at the end of the surgery. The incidence and severity of CRBD were reported at 0, 1, 2, and 6 h after arrival at the postanesthesia care unit (PACU).
Results
The incidence of CRBD was significantly lower in Groups T and L than in Group C at 1, 2, and 6 h after surgery. The incidence of moderate to severe CRBD was aslo significantly lower in Groups T and L than in Group C at 0, 1, and 2 h after surgery. The severity of CRBD reported as mild, moderate, and severe was reduced in Groups T and L compared with group C at most times after surgery. Group T had a higher incidence of nausea than Group C, and there were no differences in dizziness, drowsiness, or vomit among the three groups.
Conclusions
Tramadol and lornoxicam administered intravenously at the end of the surgery were both effective in preventing the incidence and severity of CRBD after uterine surgery. However, tramadol increased the incidence of nausea compared with saline, but there was no difference between tramadol and lornoxicam.
Trial registration:
ChiCTR2100052003. Registered on 12/10/2021.
A Recommended Novel Green Economic Sample Preparation for Developed Verified Chromatographic Assays to Determine a Common Insecticidal Blend’s Residues on Samples of Natural Nutrition and Commercial Formulation for Governmental Guarantee in the Middle East
Authors: Hegazy Amira , Mahmoud Hamada , Elsayed Mohamed , Abdelfatah Rehab 
Published: August 2022

Abstract
Spraying a tertiary blend of the insecticides; hexythiazox, imidacloprid, and thiamethoxam, on tomato fruits, are routine in agriculture-attentive countries, especially in the Middle East region, to protect them against insects’ attacks. Insecticide residuals represent one of the major environmental contamination and health risks. Specific HP-TLC and RP-HPLC methodologies are established to estimate the residual insecticides and subsequently unassuming sample collection procedures. In the planner chromatographic methodology, methanol: chloroform: glacial acetic acid: triethyl amine (8.5: 1.5: 0.2: 0.1, v/v) is recommended as a mobile system. The other one is columnar chromatography; acetonitrile: water (20: 80, v/v), pH 2.8, is recommended as a mobile system. The validation parameters are examined following the ICH rules. They are applied to field samples for estimating the insecticides’ residues, effectively.
BrWAX3, encoding a β-ketoacyl-CoA synthase, participates in cuticular wax biosynthesis in Chinese cabbage
Authors: Yang Shuangjuan , Wang Zhaojun , Tang Hao , Wei Xiaochun , Zhao Yanyan , Wang Zhi-yong , Su Henan , Niu Liujing , Yuan Yuxiang , Zhang Xiaowei 
Published: August 2022

Abstract
In this study, we identified a novel glossy mutant from Chinese cabbage, named SD369, all wax monomers longer than 26 carbons were significantly decreased. Inheritance analysis revealed that the glossy trait of SD369 was controlled by a single recessive locus, BrWAX3. We fine-mapped the BrWAX3 locus to an interval of 161.82 kb on chromosome A09. According to the annotated genome of Brassica rapa, Bra024749 (BrCER60.A09), which encodes a β-ketoacyl-CoA synthase, was identified as the candidate gene. Expression analysis showed that BrCER60.A09 was significantly downregulated in all aerial organs of glossy plants. Subcellular localization indicated that the BrCER60.A09 protein functions in the endoplasmic reticulum. A 5,567-bp insertion was identified in exon 1 of BrCER60.A09 in SD369, which lead to a premature stop codon and thus causing a loss of function of the BrCER60.A09 enzyme. Moreover, comparative transcriptome analysis revealed that the 'cutin, suberine and wax biosynthesis' pathway was significantly enriched, and genes involved in this pathway were almost upregulated in glossy plants. Further, two functional markers, BrWAX3-InDel and BrWAX3-KASP1, were developed and validated. Overall, these results provide new information for the cuticular wax biosynthesis and provide applicable markers for marker-assisted selection (MAS)-based breeding of Brassica rapa.
Self-rated health and pension benefit adequacy: Evidence from Poland
Authors: Jajko-Siwek Alicja 
Published: August 2022

Abstract

Background: The epidemic situation in the world in 2020 and 2021 raises many questions about people’s health. It is particularly important to identify the health of the population at high risk, including elderly individuals, especially retired people. We aim to identify how pension adequacy is related to the subjective health assessment of pensioners in Poland. Methods: The study uses data from the SHARE 50+ study in Europe, and, more specifically, from the SHARELIFE questionnaire results. The considered sample of 533 Polish pensioners was divided into persons receiving adequate and inadequate pension benefits. The study was conducted using the method of decision trees constructed according to the CART algorithm. Results: People with adequate benefits are healthy and rarely visit a doctor. If they are suffering from depression, they have frequent medical appointments. In the inadequate pensioners’ group, good self-rated health is declared only by people who visit the doctor no more than twice a year. Conclusions: Self-rated health is independent of the adequacy of pension benefits. Both women and men similarly rate their health. The decisive factor in the context of self-rated health is the intensity of physical and mental disease symptoms, the age of pensioners, and the availability of medical services.
Giant Perianal Tumour Arising from Condyloma Acuminatum in a Patient Living with HIV-1
Authors: Liu Xiang , Zhu Xueling , Wan Zhikai , Lang Guanjing , Huang Ying , Zhu Biao 
Published: August 2022

Abstract
Background
Perianal cancer is a relatively rare disease, but it is prevalent in men who have sex with men and in patients who are positive for human immunodeficiency virus (HIV). Here, we report a case of a massive anal squamous cell carcinoma that measured 19 cm in length and 10 cm in diameter in a male patient living with HIV.
Case presentation
A 28-year-old man with a 5-year history of peri-anal condyloma acuminatum developed a rapidly enlarging mass in the anal region since the past few months. He had both HIV and syphilis infection, but never sought further treatment. Pathological analysis and immunohistochemistry confirmed squamous cell carcinoma with high-risk HPV infection. After multi-disciplinary treatment, albumin-paclitaxel combined with anti-programmed cell death protein 1 therapy and simultaneous antiretroviral therapy was initiated. The mass began to shrink after chemotherapy, but this did not prevent tumor progression. He eventually died from tumor-cachexia.
Conclusion
Early screening and treatment of perianal disease can help prevent progression to invasive anal carcinoma in high-risk groups such as men who have sex with men and immunosuppressed patients.
Successful Managing of the Covid-19 Pandemic: Lessons Learned from Taiwan
Authors: Halide Halmar , Andika Andika , Wulandari Putri 
Published: August 2022

Abstract
Covid-19 pandemic badly affects most countries in the world both sociologically and economically. Taiwan, however, manages to handle its pandemic remarkably well before and after its Covid-19 cases spike. This paper aims to describe Taiwan’s strategy on keeping the cases low and to identify significant factors related to this spike. These factors are found through the use of a stepwise regression model. The model inputs are daily data sets that belong to the three grouped indicators: Containment and closures, Economic responses and Health systems. In addition, the number of Covid-19 tests and changes in the number of people attending indoor and outdoor activities are also given as model inputs. The model output is the daily covid-19 confirmed cases. It is found that restriction in internal move, government campaign, debt or contract relieved, indoor activities changes, work closures and their interactions are amongst the most significant factors leading to the sharp jump in Covid-19 cases. The model is able to explain almost 88% of the cases. It is important that these factors are taken into consideration by any government in its preparation for an upcoming pandemic.
Advances in mechanistic study of control of oxidative stress injury through modulating HDAC6 activity
Authors: Xue Yuanye , Gan Bing , Zhou Yanxing , Wang Tingyu , Zhu Tong , Zhou Yanfang , Zhang Xiangning , Peng Xinsheng 
Published: August 2022

Abstract
Oxidative stress is defined as injury resulting from the disturbance on dynamic equilibrium of redox environment due to over-production of active/ radical oxygen exceeding the anti-oxidative ability in the body. It is a key step of genesis and development of various diseases. Oxidative stress is modulated by different factors and events, including modification of histone, the core of nucleosome. Modification of histone includes acetylation and deacetylation on certain amino acid residues; the process is catalyzed by different enzymes. Histone deacetylase 6 (HDAC6) is a unique deaetylating protease; it also catalyzes deacetylation of different non-histone substrates so as to regulate various physiologic processes. The intimate relationship between HDAC6 and oxidative stress has been demonstrated by different lines of study. The present paper aims at summarizing the data obtained from mechanistic study between HDAC6 and oxidative stress, in order to provide guidance for further investigations in term of mechanistic characterization and drug development.
Neurophysiological Effects of Cognitive Behavioral Therapy in Social Anxiety: An ERP Study
Authors: Song Sutao , Jiang Ting , Zhao Shimeng , Zhang Mingxian , Feng Jing , Zheng Yuanjie , Ge Ruiyang 
Published: August 2022

Abstract
Background: Social anxious individuals show attention bias towards emotional stimuli, this phenomenon is considered to be an important cause of anxiety generation and maintenance. Cognitive-behavioral therapy (CBT) is a standard psychotherapy for social anxiety disorder. CBT decreases attention biases by correcting the maladaptive beliefs of socially anxious individuals, but it is not clear whether CBT alters neurophysiological features of socially anxious individuals at early automatic and/or late cognitive strategy stage of attentional processing.Method: To address this knowledge gap, we collected pre-treatment event-related potential data of socially anxious individuals while they performed a dot-probe task. These participants then received eight weeks of CBT, and post-treatment ERP data were collected after completion of CBT treatment. Results: Participants’ social anxiety level was significantly alleviated with CBT. ERP results revealed that (1) compared to pre-treatment phase, P1 amplitudes induced by probes significantly decreased at post-treatment phase, whereas P3 amplitudes increased at post-treatment phase; (2) amplitude of components elicited by face pairs did not change significantly between pre-treatment and post-treatment phases; (3) changes of Liebowitz Social Anxiety Scale were positively correlated with changes of P1 amplitude, and negatively correlated with changes of N1 amplitude.Limitations: Our sample was university students, which limits the generalizability of the results.Conclusion: The present results demonstrated that CBT alters ERPs appeared in probe-presenting stage for social anxiety, which suggested that key to the effective intervention was the adjustment of cognitive strategies in the later stage of attentional processing.
A novel unified ranking method of Pythagorean fuzzy numbers characterized by the residual sector area
Authors: Li Yanhong , Sun Gang , Li Xiaoping 
Published: August 2022

Abstract
Pythagorean fuzzy number (PFN) is not only an extension of traditional intuitionistic fuzzy number (IFN), but also can deal with the decision-making problem of multi-attribute index information in a wider range. In recent years, it has been rapidly developed and popularized in the field of decision science. In this paper, it was pointed out that there were many defects in some existing score functions and ranking criteria of PFNs through counter examples, and the main causes of these defects were analyzed with some examples. All IFNs and PFNs are unified in the Pythagorean fuzzy environment, by calculating the residual sector area(RSA) of the geometric figure corresponding to PFN and its degree of hesitation, a new score function and ranking criterion are proposed, the rationality of the ranking criterion are proved, and the basic properties of the score function are discussed. In addition, a new score function and its ranking method were proposed in the Pythagorean fuzzy environment, which ended the chaotic situation of independent ranking of IFNs and PFNs. It only needs a score function to rank all PFNs perfectly, especially for some equivalent PFNs, which can further realize accurate comparison, so as to overcome the contradiction between other methods and traditional IFN’s methods. Therefore, the proposed method has deep practical background and theoretical significance, and is more universal, practical and scientific. However, the new score function has not been applied to the decision-making problem of multi-attribute information. Maybe it needs to improve and continue to explore other excellent properties. This also provides a theoretical basis for the study of the wide application of Pythagorean fuzzy sets. (1) The ranking methods of IFNs and PFNs are contradictory. Since PFN is a generalization of IFN, the method given in [14,15] should be suitable for the method in [2,3], such as, let (0.5,0.3)) () 0.5 0.3 0.16 0.15 0.
Multimodal sensor fusion in the latent representation space
Authors: Piechocki Robert , Wang Xiaoyang , Bocus Mohammud 
Published: August 2022

Abstract
A new method for multimodal sensor fusion is introduced. The technique relies on a two-stage process. In the first stage, a multimodal generative model is constructed from unlabelled training data. In the second stage, the generative model serves as a reconstruction prior and the search manifold for the sensor fusion tasks. The method also handles cases where observations are accessed only via subsampling i.e. compressed sensing. We demonstrate the effectiveness and excellent performance on a range of multimodal fusion experiments such as multisensory classification, denoising, and recovery from subsampled observations.
Characterization of drinking groundwater quality and assessment of human health risk in Xin'an spring basin, a typical mining and karst area of the northern China
Authors: Zhang Chunchao , Li Xiangquan , Hou Xinwei , Wang Zhenxing , Ma Jianfei , Gao Ming , Fu Changchang , Bai ZhanXue , Gui Chunlei , Zuo Xuefeng 
Published: August 2022

Abstract
Groundwater is an important natural resources of drinking water in mining area in Xin’an spring basin, northern China. To foster the sustainable development and utilization of groundwater, a total of 332 groundwater samples, including 147 pore groundwater, 95 fissure groundwater and 90 karst groundwater, were collected and hydrochemical parameters were applied to assess the groundwater quality and human health risks caused by oral ingestion. The Nemerow comprehensive index (NCI), water quality index (WQI) and human health risk assessment (HRA) were used to comprehensively evaluate groundwater quality. Monte-Carlo simulation was a capable way to analyze the sensitivity in the human health risks assessment, including non-carcinogenic risks and carcinogenic risks. The results showed that the major chemical components of groundwater in Xin’an spring Basin were HCO3−, SO42−, NO3−, Cl−, Ca2+, Mg2+, Na+, TH, and TDS. The contents of TDS, TH, SO42−, NO3−, F−, Fe, Mn, Pb, and As in groundwater were exceed the standard of groundwater quality (SGQ) limits. The main hydrochemical types of groundwater were HCO3-Ca·Mg and SO4·Cl-Ca·Mg. The factors controlling the groundwater chemistry mainly were mineral weathering, evaporation, cation exchange, and anthropogenic input. The results of water quality assessment showed that the karst and fissure groundwater in study area was mainly excellent-good quality water, while pore groundwater was mainly good-medium quality water. The poor-extremely poor quality water was mainly distributed in Changzhi basin, and the greater contribution made by Pb, Mn, NO3−, Fe, SO42−, TH and TDS. The range of hazard index (HI) values of groundwater was 0.28 to 27.79, and the HI values mainly contributed by NO3−, F−, As, IR and ED. The cumulative carcinogenic risk (CCR) values ranged from 1.5×10− 7 to 2.78×10− 3, and the greater contribution made by Cr, As, Cd, IR and ED. The results of health risk assessment suggested that some parts of the study region may pose a significant non-carcinogenic risk and a high probability in developing cancer to local residents, and infants were at greatest risk to health, followed by children and adults, while teenagers were at least at risk.