bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictive value of magnetoencephalography in guiding the intracranial implant strategy for intractable epilepsy
Authors: Anand Adrish , Magnotti John F. , Smith David N. , Gadot Ron , Najera Ricardo A. , Hegazy Mohamed I. R. , Gavvala Jay R. , Shofty Ben , Sheth Sameer A. 
Published: April 2022

OBJECTIVE
Magnetoencephalography (MEG) is a useful component of the presurgical evaluation of patients with epilepsy. Due to its high spatiotemporal resolution, MEG often provides additional information to the clinician when forming hypotheses about the epileptogenic zone (EZ). Because of the increasing utilization of stereo-electroencephalography (sEEG), MEG clusters are used to guide sEEG electrode targeting with increasing frequency. However, there are no predefined features of an MEG cluster that predict ictal activity. This study aims to determine which MEG cluster characteristics are predictive of the EZ.

METHODS
The authors retrospectively analyzed all patients who had an MEG study (2017–2021) and underwent subsequent sEEG evaluation. MEG dipoles and sEEG electrodes were reconstructed in the same coordinate space to calculate overlap among individual contacts on electrodes and MEG clusters. MEG cluster features—including number of dipoles, proximity, angle, density, magnitude, confidence parameters, and brain region—were used to predict ictal activity in sEEG. Logistic regression was used to identify important cluster features and to train a binary classifier to predict ictal activity.

RESULTS
Across 40 included patients, 196 electrodes (42.2%) sampled MEG clusters. Electrodes that sampled MEG clusters had higher rates of ictal and interictal activity than those that did not sample MEG clusters (ictal 68.4% vs 39.8%, p < 0.001; interictal 71.9% vs 44.6%, p < 0.001). Logistic regression revealed that the number of dipoles (odds ratio [OR] 1.09, 95% confidence interval [CI] 1.04–1.14, t = 3.43) and confidence volume (OR 0.02, 95% CI 0.00–0.86, t = −2.032) were predictive of ictal activity. This model was predictive of ictal activity with 77.3% accuracy (sensitivity = 80%, specificity = 74%, C-statistic = 0.81). Using only the number of dipoles had a predictive accuracy of 75%, whereas a threshold between 14 and 17 dipoles in a cluster detected ictal activity with 75.9%–85.2% sensitivity.

CONCLUSIONS
MEG clusters with approximately 14 or more dipoles are strong predictors of ictal activity and may be useful in the preoperative planning of sEEG implantation.
Pelatihan Motivasi Dalam Rangka Meningkatkan Jiwa Wirausaha Pada Anggota Koperasi Karya Cipta Mandiri
Authors: Nani Nani , Wulansari Retno , Karlina Dira , Susanti Novia , Rahman Fakung 
Published: April 2022

Kegiatan pegabdian kepada masyarakat ini bertujuan untuk memberikan pelatihan dan motivasi kepada para anggota Koperasi Karya Cipta Mandiri supaya di masa pandemi saat ini mereka tetap semangat dan tetap kreatif dalam meningkatkan jiwa wirausaha mereka dalam berbagai usaha serta mampu memanfaatkan tantangan jadi peluang. Adapun pelaksanaan kegiatan PKM dapat berjalan dengan baik serta di sambut dengan antusias npara anggota koperasi sangat antusias penyelenggaraan PKM ini dapat diterima dengan baik oleh seluruh anggota koperasi. Metode kegiatan yang digunakan adalah bekerjasama dengan Koperasi Karya Cipta Mandiri dan melakukan tatap muka secara online melalui Google Meet serta sesi tanya jawab dan mencari solusi pemasalahan yang mereka hadapi saat ini. Selain itu dengan memberikan motivasi ke seluruh anggota koperasi supaya lebih kreatif dan inovatif dalam mengembangkan usahanya dengan mencari alternative seperti melakukan diversifikasi produk atau menciptakan lagi produk-produk yang saat ini di butuhkan masyarakat.
INVESTIGATING STUDENTS’ PERSPECTIVES ON THE USE OF TIKTOK AS AN INSTRUCTIONAL MEDIA IN DISTANCE LEARNING DURING PANDEMIC ERA
Authors: Afidah Nurul , Sari Novi Kumala , Hanifah Hanifah 
Published: April 2022

Abstract
This study aims to investigate on students’ perspective on the use of TikTok Application as supplementary instructional media during pandemic session. 20 students of English Department Student at UNWAHA were voluntary to take part in this descriptive study. Employing questionnaire was carried out to obtain data related to their opinion. The researcher collects the data through questionnaire. Then, the researcher selects and identifies the data. After selecting the data, the researcher displays those data into good sentences. 3) After displaying data, the conclusion is drawn. This questionnaire is analyzed by using descriptive statistics The findings revealed that students at UNWAHA second-semester give positive attitudes towards introducing TikTok as video aids into EFL classroom teaching meanwhile using it as an English learning strategy out of class. Also, they stated that TikTok is expected to be used as instructional media in speaking class.
Pembelajaran Matematika Menggunakan Bahan Ajar Berbasis Pendidikan Karakter Pada Era New Normal
Authors: Verdianingsih Eliza , Hayati Indah Ismi , Umardiyah Fitri 
Published: April 2022

The purpose of this research is to design appropriate character education-based teaching materials to help students learn and train math skills. This research uses Research and Development method adapted in addie model development. The subjects of this study were grade VIII of students at MTs Al-Ihsan Kalikejambon school year 2020/2021. The data collection techniques exist;ah interviews and questionnaires. Researchers designed a teaching material in the form of teaching materials based on character education. The results of this study showed that (1) Validation of character education-based teaching materials developed by expert assessments obtained a percentage of 80.4% with a fairly valid category. (2) The practicality of character education-based teaching materials by the assessment of math teachers obtains a percentage of 83.63% with a very practical category. (3) The response of students to teaching materials based on character education gets a percentage of 82.94% with a very practical category. Based on the results of the above study, the researchers concluded that character education-based teaching materials are declared valid and practical based on the assessment of experts and learners, so that character education-based teaching materials are eligible for use in mathematics learning.
Implementasi Perbup Jombang Nomor 41 Tahun 2019 Terhadap Pembentukan Karakter Spiritualitas Siswa (Studi Kasus di SDN Jombang 2)
Authors: Qurnia Nur , Malik Fatkhulloh Abdul , Sirojudin Didin 
Published: April 2022

This thesis discusses the implementation of the Jombang Regent Regulation Number 41 of 2019 on the formation of the spiritual character of students located at SDN Jombang 2. The background is the application of this regulation which is applied in public schools, while the curriculum in it contains the curriculum of Islamic boarding schools. This study aims to determine the effectiveness and factors that influence the implementation of these regulations.
This type of research is descriptive qualitative where the researcher conducts observations, interviews and documentation in order to obtain the data needed for research.
The application of these regulations is considered effective in increasing the spirituality of the students of SDN Jombang 2 although there are several influencing factors, both internal and external.
Pre-Hospital Early Warning Scores are Associated with Requirement for Medical Retrieval Services
Authors: Smith Jeremy , Andrew Emily , Smith Karen 
Published: April 2022

Objective Prehospital early warning scores (EWSs) can accurately identify patients at risk of clinical deterioration. We hypothesised that EWSs can identify patients during the prehospital phase who will subsequently require clinical escalation via medical retrieval. Methods A retrospective observational study of adult patients attended in 2018 by Ambulance Victoria in rural regions was conducted. We calculated EWSs using National Early Warning Score 2 (NEWS2) and Rapid Emergency Medicine Score (REMS) methods. Primary outcome was activation of Adult Retrieval Victoria (ARV) within 24h of ambulance attendance. We evaluated sensitivity and specificity for each score, and used multivariable logistic regression analysis to assess the independent association between EWSs and ARV activation. Results A total of 71,401 patients were included, of which 607 (0.9%) required ARV activation within 24 hours. Sensitivity and specificity of NEWS2 were 0.484 (95% CI 0.444-0.525) and 0.806 (95% CI 0.803-0.809) respectively, compared with 0.552 (95% CI 0.511-0.592) and 0.508 (95% CI 0.504-0.512) respectively for REMS. After adjustment for remoteness, distance to hospital, sex, age and hospital service level, a medium/high risk score according to the NEWS2 (OR 4.12; 95% CI 3.50-4.85, p < 0.001) and REMS (OR 2.92, 95% CI 2.26-3.77) was associated with ARV activation. Odds of ARV activation increased with remoteness and decreasing service level of the receiving hospital. Conclusions Prehospital NEWS2 and REMS were associated with medical retrieval within 24h of ambulance attendance. EWSs may allow early identification of ambulance patients requiring medical retrieval, thus facilitating earlier activation and reduced time to definitive care.
DAMPAK PEMBERLAKUKAN PEMBATASAN KEGIATAN MASYARAKAT (PPKM) LEVEL 4 TERHADAP PEDAGANG KAKI LIMA DI SUKOHARJO
Authors: Sarah Sarah Fadila , Anisa Dwi Anggraini  , An’nissa Ita Yulianti  , Denofata Swastikasari  , Lulu Fitriana Laela  , Windi Shilvia Puspitasari  , Aris Prio Agus Santoso  , Bangun Prajadi Cipto Utomo  
Published: April 2022

Abstract During the Covid 19 pandemic in Indonesia, it has weakened various sectors of life in society, including the economic sector. Various policy efforts have been carried out by the government to deal with the covid 19 virus outbreak, one of which is the government implementing PPKM level 4. People in the lower middle class such as street vendors really feel the impact that occurred because of this policy most of the street vendors experienced a drastic decrease in turnover due to the PPKM period with strict rules so that community activities were very limited and made traders hampered in the trading process. The formulation of the problem used in this study is to determine the impact caused during the Enforcement of Community Activity Restrictions (PPKM) level 4 in Sukoharjo. One of the objectives of this study was to determine the impact of level 4 Community Activity Restrictions (PPKM) on street vendors, especially in the Sukoharjo area. The method used in this study uses a case study approach with data collection sourced from primary data. The data obtained were Â analyzed qualitatively. Sources of data in this study are the results of questionnaires to the public and the results of interviews from Mr. Suraji as a basic necessities trader, Mrs. Wiji as a kebab seller, and Mr. Afthon as a Labmilk trader in Gentan. The results showed that PPKM level 4 had an impact including: (1) a very significant decrease in turnover; (2) consumers are starting to switch to online shopping instead of offline shopping: (3) unequal government assistance to merchants I.
Bioconversion of Terephthalic Acid and Ethylene Glycol Into Bacterial Cellulose by Komagataeibacter xylinus DSM 2004 and DSM 46604
Authors: Esmail Asiyah , Rebocho Ana T. , Marques Ana C. , Silvestre Sara , Gonçalves Alexandra , Fortunato Elvira , Torres Cristiana A. V. , Reis Maria A. M. , Freitas Filomena 
Published: April 2022

Komagataeibacter xylinus strains DSM 2004 and DSM 46604 were evaluated for their ability to grow and produce bacterial cellulose (BC) upon cultivation on terephthalic acid (TA) and ethylene glycol (EG), which are monomers of the petrochemical-derived plastic polyethylene terephthalate (PET). Both strains were able to utilize TA, EG, and their mixtures for BC synthesis, with different performances. K. xylinus DSM 2004 achieved higher BC production from TA (0.81 ± 0.01 g/L), EG (0.64 ± 0.02 g/L), and TA + EG mixtures (0.6 ± 0.1 g/L) than strain DSM 46604. The latter was unable to utilize EG as the sole carbon source and reached a BC production of 0.16 ± 0.01 g/L and 0.23 ± 0.1 g/L from TA alone or TA + EG mixtures, respectively. Further supplementing the media with glucose enhanced BC production by both strains. During cultivation on media containing TA and EG, rapid pH drop due to metabolization of EG into acidic compounds led to some precipitation of TA that was impregnated into the BC pellicles. An adaptation of the downstream procedure involving BC dissolution in NaOH was used for the recovery of pure BC. The different medium composition tested, as well as the downstream procedure, impacted the BC pellicles’ physical properties. Although no variation in terms of the chemical structure were observed, differences in crystallinity degree and microstructure of the produced BC were observed. The BC produced by K. xylinus DSM 2004 had a higher crystallinity (19–64%) than that of the strain DSM 46604 (17–53%). Moreover, the scanning electron microscopy analysis showed a higher fiber diameter for K. xylinus DSM 2004 BC (46–56 nm) than for K. xylinus DSM 46604 (37–49 nm). Dissolution of BC in NaOH did not influence the chemical structure; however, it led to BC conversion from type I to type II, as well as a decrease in crystallinity. These results demonstrate that PET monomers, TA and EG, can be upcycled into a value-added product, BC, presenting an approach that will contribute to lessening the environmental burden caused by plastic disposal in the environment.
Industrial Biotechnology Based on Enzymes From Extreme Environments
Authors: Mesbah Noha M. 
Published: April 2022

Biocatalysis is crucial for a green, sustainable, biobased economy, and this has driven major advances in biotechnology and biocatalysis over the past 2 decades. There are numerous benefits to biocatalysis, including increased selectivity and specificity, reduced operating costs and lower toxicity, all of which result in lower environmental impact of industrial processes. Most enzymes available commercially are active and stable under a narrow range of conditions, and quickly lose activity at extremes of ion concentration, temperature, pH, pressure, and solvent concentrations. Extremophilic microorganisms thrive under extreme conditions and produce robust enzymes with higher activity and stability under unconventional circumstances. The number of extremophilic enzymes, or extremozymes, currently available are insufficient to meet growing industrial demand. This is in part due to difficulty in cultivation of extremophiles in a laboratory setting. This review will present an overview of extremozymes and their biotechnological applications. Culture-independent and genomic-based methods for study of extremozymes will be presented.
β-Catenin Signaling Evokes Hair Follicle Senescence by Accelerating the Differentiation of Hair Follicle Mesenchymal Progenitors
Authors: Han Jimin , Lin Kaijun , Choo Huiqin , He Jia , Wang Xusheng , Wu Yaojiong , Chen Xiaodong 
Published: April 2022

Rationale: β-catenin signaling controls multiple fibroblast subsets, with its overactivity promoting the differentiation of hair follicle dermal stem cells (hfDSCs) and the hyperactivation of interfollicular fibroblasts. Understanding the concept of hfDSC activation and modulation offers hope towards the therapeutic armamentarium in dermatology and related comorbidities, as well as their potential applications in gerontology (the study of physiological aging). Having a comprehensive understanding in this stochastic process could also further yield important, novel insights into the molecular basis of skin aging to improve lifespan and preventing aging-related diseases.Methods: A new CD34CrePGR mouse line was generated. Through fate-tracing models and a series of β-catenin genetic experiments, our study depicts how the wound environment increases phosphorylated β-catenin in hfDSCs and facilitates their differentiation into dermal papilla (DP) and dermal sheath (DS). In mice carrying hfDSC-specific activated allele of β-catenin, hfDSCs accelerated their differentiation into DP cells.Results: Notably, with β-catenin stabilization in CD34-expressing cells and potential activation of canonical Wnt signaling, the mutant mice showed a brief increase of hair density in the short term, but over time leads to a senescence phenotype developing premature canities and thinning [hair follicle (HF) miniaturization].Conclusion: β-catenin signaling drove HF senescence by accelerating differentiation of CD34+ hfDSCs, resulting in phenotypes attributable to the differentiation of the hfDSCs into DP cells and the loss of their stem cell potential. Therefore, our study reveals that the regulation of β-catenin signaling in hfDSCs may potentially become an important subject for future exploration in development of clinically effective therapies for hair loss treatment and an excellent model for revealing new therapeutic approaches to reverse aging or retarding the development of alopecia.
Antibodies Against Phosphorylcholine Among 60-Year-Olds: Clinical Role and Simulated Interactions
Authors: Samal Shailesh Kumar , Panda Pritam Kumar , Vikström Max , Leander Karin , de Faire Ulf , Ahuja Rajeev , Frostegård Johan 
Published: April 2022

AimsAntibodies against phosphorylcholine (anti-PC) are implicated as protection markers in atherosclerosis, cardiovascular disease (CVD), and other chronic inflammatory conditions. Mostly, these studies have been focused on IgM. In this study, we determined IgG, IgG1, and IgG2 anti-PC among 60-year-olds.MethodsBased on a 7-year follow-up of 60-year-olds (2,039 men and 2,193 women) from Stockholm County, we performed a nested case-control study of 209 incident CVD cases with 620 age- and sex-matched controls. Anti-PC was determined using ELISA. We predicted the binding affinity of PC with our fully human, in-house-produced IgG1 anti-PC clones (i.e., A01, D05, and E01) using the molecular docking and molecular dynamics simulation approach, to retrieve information regarding binding properties to PC.ResultsAfter adjustment for confounders, IgG and IgG2 anti-PC showed some significant associations, but IgG1 anti-PC was much stronger as a protection marker. IgG1 anti-PC was associated with an increased risk of CVD below 33rd, 25th, and 10th percentile and of stroke below 33rd and 25th, and of myocardial infarction (MI) below 10th percentile. Among men, a strong association with stroke was determined below the 33rd percentile [HR 9.20, CI (2.22–38.12); p = 0.0022]. D05 clone has higher binding affinity followed by E01 and A01 using molecular docking and further have been confirmed during the course of 100 ns simulation. The stability of the D05 clone with PC was substantially higher.ConclusionIgG1 anti-PC was a stronger protection marker than IgG anti-PC and IgG2 anti-PC and also separately for men. The molecular modeling approach helps in identifying the intrinsic properties of anti-PC clones and atomistic interactions with PC.
DeepStrain Evidence of Asymptomatic Left Ventricular Diastolic and Systolic Dysfunction in Young Adults With Cardiac Risk Factors
Authors: Morales Manuel A. , Snel Gert J. H. , van den Boomen Maaike , Borra Ronald J. H. , van Deursen Vincent M. , Slart Riemer H. J. A. , Izquierdo-Garcia David , Prakken Niek H. J. , Catana Ciprian 
Published: April 2022

PurposeTo evaluate if a fully-automatic deep learning method for myocardial strain analysis based on magnetic resonance imaging (MRI) cine images can detect asymptomatic dysfunction in young adults with cardiac risk factors.MethodsAn automated workflow termed DeepStrain was implemented using two U-Net models for segmentation and motion tracking. DeepStrain was trained and tested using short-axis cine-MRI images from healthy subjects and patients with cardiac disease. Subsequently, subjects aged 18–45 years were prospectively recruited and classified among age- and gender-matched groups: risk factor group (RFG) 1 including overweight without hypertension or type 2 diabetes; RFG2 including hypertension without type 2 diabetes, regardless of overweight; RFG3 including type 2 diabetes, regardless of overweight or hypertension. Subjects underwent cardiac short-axis cine-MRI image acquisition. Differences in DeepStrain-based left ventricular global circumferential and radial strain and strain rate among groups were evaluated.ResultsThe cohort consisted of 119 participants: 30 controls, 39 in RFG1, 30 in RFG2, and 20 in RFG3. Despite comparable (&gt;0.05) left-ventricular mass, volumes, and ejection fraction, all groups (RFG1, RFG2, RFG3) showed signs of asymptomatic left ventricular diastolic and systolic dysfunction, evidenced by lower circumferential early-diastolic strain rate (&lt;0.05, &lt;0.001, &lt;0.01), and lower septal circumferential end-systolic strain (&lt;0.001, &lt;0.05, &lt;0.001) compared with controls. Multivariate linear regression showed that body surface area correlated negatively with all strain measures (&lt;0.01), and mean arterial pressure correlated negatively with early-diastolic strain rate (&lt;0.01).ConclusionDeepStrain fully-automatically provided evidence of asymptomatic left ventricular diastolic and systolic dysfunction in asymptomatic young adults with overweight, hypertension, and type 2 diabetes risk factors.
Effect of High-Intensity Interval Training, Moderate Continuous Training, or Guideline-Based Physical Activity on Peak Oxygen Uptake and Myocardial Fibrosis in Patients With Myocardial Infarction: Protocol for a Randomized Controlled Trial
Authors: Shi Xiaohe , Chen Xianyuan , Qiu Xinfan , Luo Wei , Luo Xinyi , Liu Hui , Geng Qingshan , Ma Huan , Xue Ling , Guo Lan 
Published: April 2022

IntroductionHigh-intensity interval training (HIIT) is an emerging method of cardiac rehabilitation, which is more and more popular in recent years. Research into the effect of HIIT on peak oxygen uptake (VO2 peak) and myocardial fibrosis among patients with myocardial infarction (MI) is lacking. Here, we describe the rationale along with the protocol for a clinical trial to test the following hypotheses: (1) compared with the control group, VO2 peak will be increased in both the moderate-intensity continuous training (MICT) and HIIT groups and (2) compared with the control group, myocardial fibrosis due to MI will be improved by HIIT and MICT.Methods and AnalysisThis is a single-center, randomized controlled clinical trial. In total, 180 patients with MI are to be recruited for this study. VO2 peak will be tested by cardiopulmonary exercise testing (CPET) and myocardial fibrosis will be evaluated by cardiac MR. A variety of blood and psychometric tests and also the peripheral arterial tonometry, reactive hyperemia index for microvascular endothelial function, and microvascular blockage or digital vasomotor response are included.Ethics and DisseminationThe ethics committee of the Guangdong Provincial People's Hospital has authorized this mechanistic clinical research. Peer-reviewed articles and conference presentations will be used to disseminate the findings.Trial Registration NumberNCT04863677.