bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lessons from comparative primatology for understanding trait covariation and diversity in evolutionary ecology
Authors: Correia Isadora , Gomes Bruna de França , Villalobos Fabricio , Ferrari Stephen F. , Gouveia Sidney F. 
Published: December 2022

ABSTRACTGiven the position of humans in the tree of life, comparative research on non‐human primates has attracted the interest of researchers in biology, medical sciences, anthropology, psychology, and sociology. Covariation of species' phenotypes has been of particular interest.Learning from the historical development of comparative research with primates should thus be particularly valuable for evolutionary ecology and to improve understanding of phenotypic integration and diversity. Such learning would also help identify knowledge gaps, disputed questions, and new avenues of both basic and applied research in relation to the evolution of primate features and the conservation of our close relatives.We conducted a historical assessment through a non‐systematic review and a systematic review, focusing on how the integration of different research lines in evolutionary ecology focused on primate phenotypic covariation unfolded throughout the 20th Century. The non‐systematic review allowed us to reconstruct the history of the discipline from its earliest origins, when bibliometric assessments were more limited in scope, and to identify the most appropriate keywords for the systematic review. We employed a standard protocol for the systematic review, applying two complementary analyses: co‐occurrence of keywords and bibliographic coupling of references. These analyses described the development of the conceptual and intellectual structures of comparative primatology from 1966 to 2020.By identifying the most influential researchers and concept interrelations, we highlight primate phenotypes critical for the development of the discipline (in particular, brain and body sizes and behavioural patterns), showcasing the reach of these investigations for evolutionary ecology. Overall, our findings emphasise the crucial role that comparative primatology has played in developing the study of phenotypic integration and the very onset of phylogenetic comparative methods.
Quality standards and recommendations for research in music and neuroplasticity
Authors: Papatzikis Efthymios , Agapaki Maria , Selvan Rosari Naveena , Pandey Varun , Zeba Fathima 
Published: December 2022

AbstractResearch on how music influences brain plasticity has gained momentum in recent years. Considering, however, the nonuniform methodological standards implemented, the findings end up being nonreplicable and less generalizable. To address the need for a standardized baseline of research quality, we gathered all the studies in the music and neuroplasticity field in 2019 and appraised their methodological rigor systematically and critically. The aim was to provide a preliminary and, at the minimum, acceptable quality threshold—and, ipso facto, suggested recommendations—whereupon further discussion and development may take place. Quality appraisal was performed on 89 articles by three independent raters, following a standardized scoring system. The raters’ scoring was cross‐referenced following an inter‐rater reliability measure, and further studied by performing multiple ratings comparisons and matrix analyses. The results for methodological quality were at a quite good level (quantitative articles: mean = 0.737, SD = 0.084; qualitative articles: mean = 0.677, SD = 0.144), following a moderate but statistically significant level of agreement between the raters (W = 0.44, χ2 = 117.249, p = 0.020). We conclude that the standards for implementation and reporting are of high quality; however, certain improvements are needed to reach the stringent levels presumed for such an influential interdisciplinary scientific field.
Profiling of phytohormone‐specific microRNAs and characterization of the miR160‐ARF1 module involved in glandular trichome development and artemisinin biosynthesis in Artemisia annua
Authors: Guo Zhiying , Hao Kai , Lv Zongyou , Yu Luyao , Bu Qitao , Ren Junze , Zhang Henan , Chen Ruibing , Zhang Lei 
Published: December 2022

SummaryMicroRNAs (miRNAs) play crucial roles in plant development and secondary metabolism through different modes of sequence‐specific interaction with their targets. Artemisinin biosynthesis is extensively regulated by phytohormones. However, the function of phytohormone‐responsive miRNAs in artemisinin biosynthesis remains enigmatic. Thus, we combined the analysis of transcriptomics, small RNAs, and the degradome to generate a comprehensive resource for identifying key miRNA‐target circuits involved in the phytohormone‐induced process of artemisinin biosynthesis in Artemisia annua. In total, 151 conserved and 52 novel miRNAs and their 4132 targets were determined. Based on the differential expression analysis, miR160 was selected as a potential miRNA involved in artemisinin synthesis. Overexpressing MIR160 significantly impaired glandular trichome formation and suppressed artemisinin biosynthesis in A. annua, while repressing its expression resulted in the opposite effect, indicating that miR160 negatively regulates glandular trichome development and artemisinin biosynthesis. RNA ligase‐mediated 5′ RACE and transient transformation assays showed that miR160 mediates the RNA cleavage of Auxin Response Factor 1 (ARF1) in A. annua. Furthermore, ARF1 was shown to increase artemisinin synthesis by activating AaDBR2 expression. Taken together, our results reveal the intrinsic link between the miR160‐ARF1 module and artemisinin biosynthesis, and may expedite the innovation of metabolic engineering approaches for high and stable production of artemisinin in the future.
Group VII ethylene response factors, MtERF74 and MtERF75, sustain nitrogen fixation in Medicago truncatula microoxic nodules
Authors: Rovere Martina , Pucciariello Chiara , Castella Claude , Berger Antoine , Forgia Marco , Guyet Tran A. , Bosseno Marc , Pacoud Marie , Brouquisse Renaud , Perata Pierdomenico , Boscari Alexandre 
Published: December 2022

AbstractGroup VII ethylene response factors (ERF‐VII) are plant‐specific transcription factors (TFs) known for their role in the activation of hypoxia‐responsive genes under low oxygen stress but also in plant endogenous hypoxic niches. However, their function in the microaerophilic nitrogen‐fixing nodules of legumes has not yet been investigated. We investigated regulation and the function of the two Medicago truncatula ERF‐VII TFs (MtERF74 and MtERF75) in roots and nodules, MtERF74 and MtERF75 in response to hypoxia stress and during the nodulation process using an RNA interference strategy and targeted proteolysis of MtERF75. Knockdown of MtERF74 and MtERF75 partially blocked the induction of hypoxia‐responsive genes in roots exposed to hypoxia stress. In addition, a significant reduction in nodulation capacity and nitrogen fixation activity was observed in mature nodules of double knockdown transgenic roots. Overall, the results indicate that MtERF74 and MtERF75 are involved in the induction of MtNR1 and Pgb1.1 expression for efficient Phytogb‐nitric oxide respiration in the nodule.
The gender gap in pro‐environmental political participation among older adults
Authors: El Khoury Cristina , Felix Amédée , Lorenzini Jasmine , Rosset Jan 
Published: December 2022

AbstractCitizens are increasingly concerned with environmental issues and some of them take contentious political actions or adapt their lifestyles to reduce their environmental footprint. Research finds that there is an eco‐gender gap in everyday environmental behaviours, with women more active than men. However, studies of the eco‐gender gap have infrequently looked at other forms of pro‐environmental political behaviours and focused mostly on young people. We contribute to the literature by examining to what extent older women and men differ in a range of pro‐environmental political behaviours, using a unique dataset based on a survey of individuals 64 years and older. We find strong evidence of a gender gap in everyday behaviours and political consumerism, but not in relation to contentious forms of political participation. This gender gap in relation to everyday behaviours and political consumerism diminishes with age.
Proposal of facial pigmentary unit and facial hyperpigmentation type for Fitzpatrick skin types II‐IV
Authors: Oh Seung‐Min , Lee Ye Eun , Ko Min Ji , Baek Ji Hwoon , Shin Min Kyung 
Published: December 2022

AbstractBackgroundStudies on facial hyperpigmentation across different facial units are limiting. We aimed to analyze melanin pigmentation images to observe facial pigmentary demarcation lines (FPDLs) and suggest facial hyperpigmentation types for normal individuals.Materials and methods3D facial melanin pigmentation images of 173 volunteers were obtained and analyzed for the presence of FPDLs. Pigmentation severity was assessed for each of the thirteen facial pigment units. The images were then grouped according to a pattern of hyperpigmentation to suggest three facial hyperpigmentation types—dark spot, photoaging and post‐inflammatory hyperpigmentation.ResultsFour groups of FPDLs including a novel group I were observed. Nasal, frontal, auricular were the darkest pigmented facial pigment unit, and the anterior neck was the least pigmented. The dark spot type was the most common facial hyperpigmentation type. The photoaging type and the PIH type showed age‐dependent distribution, as the photoaging type was more common among the subjects over 40s, and the PIH type was more common in younger subjects.ConclusionFacial hyperpigmentation among healthy individuals with Fitzpatrick skin types II‐IV is often accompanied by FPDLs and categorized into three types. Each type is modeled after the pattern of pigmentation associated with certain dermatological disorders. The practical implications of facial hyperpigmentation types can be resourceful in various fields including prevention and treatment of pigmentary disorders.
T‐box transcription factor 2 mediates antitumor immune response in cutaneous squamous cell carcinoma by regulating the expression of programmed death ligand 1
Authors: Wang Xu , Li Zhi , Sun Yadi 
Published: December 2022

AbstractBackgroundCutaneous squamous cell carcinoma (CSCC) is the second largest nonmelanoma skin cancer in humans; effective treatment options for metastatic CSCC are still in short. In this study, we aimed to explore the function of T‐box transcription factor 2 (TBX2) in CSCC.MethodsThe expression level of TBX2 was determined in CSCC samples and cell lines. Programmed death ligand 1 (PD‐L1) expression was also analyzed in human CSCC samples. Furthermore, SCC13 cells were transfected with TBX2‐DN (loss of function) or normal TBX2 to check its role in regulating PD‐L1.ResultsThe expression level of TBX2 was positively correlated with the stage of CSCC. CSCC tumor cell lines have significantly higher expression levels of TBX2 than normal skin cell lines, and SCC13 cells showed the highest expression. PD‐L1 expressions were upregulated during the progression of CSCC, and positively correlated with TBX2. Furthermore, PD‐L1 expression increased in SCC13 cells overexpressing TBX2. However, TBX2 did not regulate the activation of IFNγ signal, but mediated the expression of interferon regulatory factor 1 (IRF1) and PD‐L1 in both SCC13 and PDV cells.ConclusionTBX2 could mediate antitumor immune response in CSCC by regulating the expression of PD‐L1 through IRF1. It might be a prognostic marker in CSCC and synergistic target for PD‐1 immunotherapy.
Nanoplastic–plant interaction and implications for soil health
Authors: Zhou Pingfan , Wang Liuwei , Gao Jing , Jiang Yaqi , Adeel Muhammad , Hou Deyi 
Published: December 2022

AbstractNanoplastics (NPs) are accumulating in the soil environment at a rapid rate, which may cause serious consequences for ecosystems and human health. However, environmental behaviour and toxicity of NPs in the soil–plant system remain poorly understood. This review summarizes current studies on NP–plant interactions to unravel uptake mechanisms and phytotoxicity of NPs. NPs could be taken up by plant roots and transported upwards through the xylem to all organs of the plant, even to the edible parts such as the grain, thereby threatening human health. The interaction of NPs with plants affects plant transport of water and nutrients. Besides, it induces significant oxidative stress leading to inhibition of physiological and biochemical activities such as photosynthesis, and thus adversely affects plant growth and development. In addition, the co‐transport of NPs with other soil pollutants may induce the combined toxic effects. This study also discussed the potential mechanism of NP–plant interactions based on previous experience with engineered nanomaterials. Finally, a comprehensive assessment of the key challenges in each area was presented, and future perspectives are offered.
Donor and non‐donor perspectives on receiving information from routine genomic testing of donor blood
Authors: Thorpe Rachel , Jensen Kyle , Masser Barbara , Raivola Vera , Kakkos Athina , von Wielligh Kobie , Wong Jonathan 
Published: December 2022

AbstractBackgroundGenomic testing is already used by blood collection agencies (BCAs) to identify rare blood types and ensure the best possible matching of blood. With ongoing technological developments, broader applications, such as the identification of genetic markers relevant to blood donor health, will become feasible. However, the perspectives of blood donors (and potential blood donors) on routine genomic testing of donor blood are under‐researched.Study Design and MethodsEight online Focus Groups were conducted: four with donors and four with non‐donors. Participants were presented with three hypothetical scenarios about the current and possible future applications of genomic testing: Performing rare blood type testing; identifying donors with genetic markers associated with iron metabolism; and identifying donors with genetic markers associated with bowel cancer.ResultsTesting to identify rare blood types was perceived to be an appropriate application for the BCA to undertake, while identifying markers associated with iron metabolism and cancer genetic markers were only partially supported. Participants raised concerns about the boundaries of acceptable testing and the implications of testing for privacy, data security, and health insurance. Perspectives of donors and non‐donors on all scenarios were similar.DiscussionThe principles of who benefits from genomic testing and the perceived role of BCAs were key in shaping participants' perspectives. Participants generally agreed that testing should be directly related to blood donation or be of benefit to the recipient or donor. Findings indicate that consent and communication are key to the acceptability of current and expanded genomic testing.
Feasibility Study of Rear Door Heat Exchanger for a High Capacity Data Center
Authors: Shalom Simon Vibin , Modi Himanshu , Sivaraju Krishna Bhavana , Bansode Pratik , Saini Satyam , Shahi Pardeep , Karajgikar Saket , Mulay Veerendra , Agonafer Dereje 
Published: December 2022

Abstract
Due to increased use of high-performance computing in datacenters to cater to huge workloads, old low-performance compute servers must be replaced endlessly with high-performance compute servers. Traditional air-cooling systems are insufficient to provision and run the servers in optimal conditions as the datacenter thermal footprint or rack density grows, resulting in thermal throttling. To sustain the growing needs, Rear Door Heat Exchangers (RDHx) are deployed in existing datacenters along with peripheral Computer Room Air Handling/Conditioning (CRAH/CRAC) units. RDHx transfers heat from the rear end of the racks and rejects it into the facility’s chilled water. This study will demonstrate the suitability of RDHx for low density as well as high density rack applications. A baseline CFD model had a generic datacenter layout with peripheral CRAH/CRAC units and RDHx. Several case studies were conducted by varying the air and liquid inlet temperatures for rack and RDHx, respectively. We also compared active and passive modes of operating RDHx while server fans provide flowrate based on the IT inlet temperature. The paper will also discuss the feasibility of designing a datacenter with only RDHx and no peripheral CRAC/CRAH units while maintaining the thermal envelop. The research will also provide a guideline in implementing RDHx based on the heat load and server design.
Effects of the COVID-19 pandemic on sleep health among Middle Eastern and North African (MENA) populations: a systematic review of the literature
Authors: Al-Ajlouni Yazan A , Al Ta'ani Omar , Shamaileh Ghaith , Mushasha Rand , Makarem Nour , Duncan Dustin T. 
Published: December 2022

ObjectivesThis study sought to conduct a systematic review of the literature on the impact of the COVID-19 pandemic on sleep health among Middle Eastern and North African (MENA) populations, understudied geographic regions including with regards to sleep health.SettingA systematic literature search of studies published from inception to 27 March 2022 was conducted on multiple databases using developed keywords.ParticipantsStudies were included if they (1) investigated one or more aspects/dimensions of sleep health as an outcome (eg, sleep duration, sleep quality, sleep problems); (2) measured the impact of a COVID-19 pandemic-related domain (eg, impact of quarantine, work from home, lifestyle changes); (3) focused on at least one MENA region population; (4) were peer-reviewed; (5) included ≥100 participants; (6) were written in English and (7) had full-text article publicly available.Primary and secondary outcomes measuredPrimary outcomes were sleep duration, sleep quality and sleep problems.ResultsIn line with Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, 164 studies were included for data extraction. The Newcastle-Ottawa scale for cross-sectional studies was used to assess the quality of the studies. Overall, the COVID-19 pandemic significantly impacted sleep duration, sleep quality and presence and severity of sleep disorders in MENA populations, including adults, children, students, healthcare workers and people with chronic illnesses. The directionality and strength of associations, as well as the determinants of sleep health, varied by subpopulations.ConclusionsLongitudinal studies are needed to understand the longer-term impact of the COVID-19 pandemic on the sleep health of MENA populations. Sleep health interventions and policy measures should be tailored to the need of each subpopulation.PROSPERO registration numberCRD42022321128.
Importance of quick attainment of minimal disease activity for a positive impact on lives of patients with psoriatic arthritis
Authors: Snoeck Henkemans Selinde V J , de Jong Pascal H P , Luime Jolanda J , Kok Marc R , Tchetverikov Ilja , Kasiem Fazira R , Welby Sarah , Prickett Adam R , van der Helm-van Mil Annette H M , Vis Marijn 
Published: December 2022

ObjectiveTo compare patient-reported outcomes (PROs) from the first year to the third year between patients with psoriatic arthritis (PsA) who achieved minimal disease activity (MDA) in the first year after diagnosis and those who did not.MethodsConsecutive, newly diagnosed, patients with DMARD naïve PsA with oligoarthritis or polyarthritis were selected from the Dutch southwest Early PsA cohoRt. Patients were categorised in three groups: (1) Patients who were in MDA at both 9 months and 12 months after diagnosis (sustained MDA); (2) Patients who achieved MDA in the first year but in whom it was not sustained at both 9 months and 12 months (non-sustained MDA); (3) Patients who did not achieve MDA in the first year (no MDA). PROs were compared between groups from the first year to the third year after diagnosis using a linear mixed model.Results240 patients were selected; 104 (43%) were classified as sustained MDA, 60 (25%) as non-sustained MDA and 76 (32%) as no MDA. Patients who did not achieve MDA in the first year experienced remarkably lower PROs during follow-up, compared with the sustained MDA group: health status (European Quality of life 5-Dimensions 5-Levels) was 0.23 units lower (95% CI −0.28 to −0.18), functional impairment (Health Assessment Questionnaire-Disability Index) was 0.81 units higher (95% CI 0.70 to 0.92), pain (Visual Analogue Scale) was 35.38 mm higher (95% CI 30.57 to 40.18), fatigue (Bristol Rheumatoid Arthritis Fatigue-Multidimensional Questionnaire) was 17.88 units higher (95% CI 14.60 to 21.16), and anxiety and depression (Hospital Anxiety and Depression Scale) were, respectively, 3.26 units (95% CI 2.25 to 4.27) and 4.04 units higher (95% CI 3.10 to 4.99).ConclusionFailure to achieve MDA in the first year after PsA diagnosis was associated with worse PROs that persisted over the years.
The Effect of Ex-Vivo Hyaluronic Acid on Myofibroblast and Collagen in Dupuytren Disease
Authors: SINACI Cem Berkay , ÇİÇEK Çağla , FİLİNTE Gaye , GÜVEN Ülkügül 
Published: December 2022

Background: Dupuytren disease (DD) is characterised by increased myofibroblast/fibroblast activity and type3/type1 collagen ratios. Hyaluronic acid (HA) is major component of the extracellular matrix and some studies have showed that HA limits myofibroblast activity and decreases type3/type1 collagen ratio. The aim of this study is to determine the effect of the ex-vivo application of HA on cultured fibroblasts obtained from normal and diseased tissue from patients with DD. This is the initial step towards defining the use of HA as a new approach for medical treatment of DD. Methods: Tissue samples were obtained from both healthy forearm (C) and unhealthy palmar (D) fascia of patients undergoing surgery for DD. Tissue samples were cultured and divided into four groups depending on the addition of HA [C(HA−), C(HA+), D(HA−) and D(HA+)]. The tissues were evaluated using Western blot to detect effect of HA on myofibroblast (by measuring alpha smooth muscle actin [α-SMA) and on the ratio of type3/type1 collagen by measuring collagen type1 alpha 1 Chain (COL1A1) and collagen type3 alpha 1 Chain (COL3A1). Results: The rate of the average α-SMA value in the D(HA+) group was significantly lower compared to that of the D(HA−) group. The average ratio of type3/type1 collagen in the D(HA+) group was significantly lower compared to the D(HA−) group. Conclusions: The ex-vivo application of HA on cultured fibroblasts obtained from patients with DD resulted in a decrease in myofibroblast/fibroblast activity and type3/type1 collagen ratios. This may pave the way for clinical application of HA in the treatment of DD.
Use of Triceps Tendon and Paratenon Composite Graft for Extensor Tendon Reconstruction in the Hand: A Case Report
Authors: SHIBATA Ryutaro , TAKEDA Shinsuke , YAMAUCHI Ken-ichi , HIRATA Hitoshi 
Published: December 2022

An extensor tendon defect in the hand requires reconstruction to restore extension. We report a 44-year-old woman with a 24-mm extensor tendon defect of the middle finger over the dorsum of the hand that was reconstructed using a composite graft consisting of the triceps tendon and paratenon. This composite graft from the posterior aspect of the distal arm is simple, safe and may be considered in extensor tendon reconstruction. Level of Evidence: Level V (Therapeutic)