bluuebunny/crossref_metadata_2025_split huggingface dataset:
ERK‐dependent induction of the immediate‐early gene Egr1 and the late gene Gpr50 contribute to two distinct phases of PACAP Gs‐GPCR signaling for neuritogenesis
Authors: Xu Wenqin , Dahlke Sam P. , Sung Michelle , Samal Babru , Emery Andrew C. , Elkahloun Abdel , Eiden Lee E. 
Published: June 2022

AbstractGs‐coupled GPCR‐stimulated neuritogenesis in PC12 and NS‐1 ‐ cells depends on activation of the MAP kinase ERK. Here, we examine changes in ERK activation (phosphorylation), and the time course of ERK‐dependent gene induction, to seek transcriptional determinants for this process. Quenching of ERK activation by inhibition of MEK with U0126 at any time point for at least 24 h following addition of PACAP resulted in arrest of neurite formation. Changes in the transcriptome profile throughout this time period revealed at least two phases of gene induction: an early phase dominated by induction of immediate‐early genes, and a later phase of gene induction after 4–6 h of exposure to PACAP with persistent elevation of phospho‐ERK levels. Genes induced by PACAP in both phases consisted in those whose induction was dependent on ERK (i.e., blocked by U0126), and some whose induction was blocked by the protein kinase A inhibitor H89. ERK‐dependent “late gene” transcripts included Gpr50, implicated earlier in facilitation of NGF‐induced neurite formation in NS‐1 cells. Gpr50 induction by PACAP, but not NGF, was dependent on the guanine nucleotide exchange factor RapGEF2, which has been shown to be required for PACAP‐induced neuritogenesis in NS‐1 cells. Expression of a Gpr50‐directed shRNA lowered basal levels of Gpr50 mRNA and attenuated Gpr50 mRNA and GPR50 protein induction by PACAP, with a corresponding attenuation of PACAP‐induced neuritogenesis. Gs‐GPCR‐stimulated neuritogenesis first requires immediate‐early gene induction, including that of Egr1 (Zif268/NGF1A/Krox24) as previously reported. This early phase of gene induction, however, is insufficient to maintain the neuritogenic process without ERK‐dependent induction of additional late genes, including Gpr50, upon continuous exposure to neurotrophic neuropeptide. Early (Egr1) and late (Gpr50) gene induction by NGF, like that for PACAP, was inhibited by U0126, but was independent of RapGEF2, confirming distinct modes of ERK activation by Gs‐coupled GPCRs and neurotrophic tyrosine receptor kinases, converging on a final common ERK‐dependent signaling pathway for neuritogenesis.
Land and small farmer resistance in authoritarian Egypt
Authors: Bush Ray 
Published: June 2022

AbstractThis article examines small farmer resistance in Egypt. It situates contemporary struggles in the context of the uneven success of small farmers' historical resistance to land dispossession and neoliberal reform. Struggles over land are the prime driver of rural conflict and small farmers contest state strategies that reward local elites, land owners, and supporters of the military regime. There may not be a coherent social movement able to resist contemporary patterns of capital accumulation, but there are elements of resistance that edge forward an agenda for a “political conversation” about much‐needed deep social reforms. The internationally‐supported Sisi presidency has used violence to consolidate military power, making open revolt difficult. Nevertheless, small farmers link struggles over access to land with poor state provisioning of water and infrastructure as a means of building resistance.
Participation in daily activities after acute illness hospitalization among high‐functioning older adults: a qualitative study
Authors: Arieli Maya , Kizony Rachel , Gil Efrat , Agmon Maayan 
Published: June 2022

AbstractAims and objectivesTo describe high‐functioning older adults' experiences of participation in daily activities and perceived barriers and facilitators to participation one‐ and 3‐months post‐acute hospitalization.BackgroundOlder adults discharged after acute illness hospitalization are at risk for functional decline and adverse health outcomes. Yet, little is known about the subjective experience of resuming participation in meaningful activities beyond the immediate post‐discharge period among high‐functioning older adults, a mostly overlooked sub‐sample.DesignQualitative descriptive longitudinal study adhering to the COREQ guidelines.MethodsForty two participants ages ≥65 years (mean age 75, SD ± 7.9) were recruited from internal medicine wards. Semi‐structured interviews were conducted at participants' homes one‐month post‐discharge, followed by a telephone interview 3‐months after. Data were analyzed using thematic analysis.ResultsParticipants perceived the hospitalization as a disruption of healthy and meaningful routines. This first key theme had unique expressions over time and included two sub‐themes. At one month: (1) reduced life spaces and sedentary routines. At 3 months: (2) a matter of quality not quantity – giving up even one meaningful activity can make a difference. The second key theme was described as a combination of physical and psychological barriers to participation over time. These themes demonstrated the profound impact of the hospitalization on behavior (participation) and feelings (e.g., symptoms). The third key theme was described as a dyad of intrinsic and extrinsic facilitators to participation.ConclusionsAcute illness hospitalization may lead to subtle decreases in participation in meaningful health‐promoting activities, even among high‐functioning older adults. These changes may impact overall well‐being and possibly mark the beginning of functional decline.Relevance to clinical practiceThis study highlights the need for a more comprehensive assessment of participation, relevant for high‐functioning older adults, to enable person‐centered care. Intervention programs should address the modifiable barriers and facilitators identified in this study.
Temporal Dynamics in Acquisition Behavior: The Effects of Activity Load on Strategic Momentum
Authors: Keil Thomas , Deutsch Yuval , Laamanen Tomi , Maula Markku 
Published: June 2022

AbstractMomentum theory suggests that acquisition experience leads to acquisition momentum in the form of a higher likelihood of subsequent acquisitions of the same type. However, this argument has been challenged theoretically and empirically. We reconcile conflicting predictions and findings of prior research and extend momentum theory by incorporating activity load as a novel causal mechanism to both replicate the base finding and explain deviations from it. We find that a high activity load due to increased acquisition activity acts as a counterforce to momentum, decreasing the likelihood of subsequent acquisitions of the same type. Moreover, we also find that the interplay of routines, cognitive frames, and activity load causes companies to alternate between different types of acquisitions – from small to large and from large to small – as management engages in attention modulation to preserve momentum. Taken together, our arguments and findings contribute to an improved understanding of temporal patterns of acquisition behaviour.
Gendered share of housework and the COVID‐19 pandemic: Examining self‐ratings and speculation of others in Germany, India, Nigeria, and South Africa
Authors: Obioma Ihuoma Faith , Jaga Ameeta , Raina Mahima , Asekun Wakil Ajibola , Hernandez Bark Alina S. 
Published: June 2022

AbstractThis cross‐sectional study examined gender differences between male‐ and female‐typed housework during the early COVID‐19 lockdowns in 2020. Participants in Germany, India, Nigeria, and South Africa (N = 823) rated their housework share before and during the lockdown, then speculated about the division of housework performed by men and women in general, before and post‐lockdown. Women spent more time on female‐typed tasks and men (in Nigeria and South Africa) on male‐typed tasks before and during the lockdown. Irrespective of participants’ gender, they speculated that men's and women's housework was more pronounced post‐lockdown than before, but we only found gender differences in South Africa and India. Gender role ideology (GRI) moderated the gender‒housework relationship in Germany, but gender did not moderate the paid work hours and housework relationship in any country. Our findings suggest that gendered housework persisted in these countries and raises concerns that this pattern is likely to continue post‐lockdown.
Lobbying policy makers: Share versus lottery contests
Authors: Cardona Daniel , Freitas Jenny De , Rubí‐Barceló Antoni 
Published: June 2022

AbstractIn lobbying competition to influence a policy ranging over a continuum, lottery and share contests can be regarded as two alternative conflict resolution protocols. In the lottery contest, the policy is selected probabilistically whereas in the share contest the policy choice is deterministic. Although these two contest settings are equivalent for risk‐neutral lobbyists, this equivalence fails to hold when they are risk‐averse. We demonstrate that when lobbyists have concave preferences over policies and select their policy proposals, they strategically moderate their claims only under the (probabilistic) lottery setting. This action defuses the conflict and reduces the exerted effort. Consequently, risk‐averse contestants might unanimously prefer the lottery to the share contest.
THE LA LUNA/RÍO NEGRO(.) PETROLEUM SYSTEM AT THE URDANETA WEST FIELD, LAKE MARACAIBO BASIN, NW VENEZUELA: 1D BASIN MODELLING AND SECONDARY OIL MIGRATION
Authors: Escobar M. , Díaz J.G. , Márquez G. , Boente C. , Tocco R. 
Published: June 2022

This paper investigates the timing of hydrocarbon generation in the northern part of the Urdaneta West field in the NW of the Lake Maracaibo Basin, NW Venezuela, based on 1D basin modelling at three wells referred to as wells X, Y and Z. Kitchen areas were identified and secondary migration directions were inferred based on analyses of the thermal and burial history of the Upper Cretaceous La Luna Formation source rock and the geochemistry of 20 oil samples from the Río Negro Formation reservoir. Aliphatic hydrocarbons in the oil samples were analysed by gas chromatography – mass spectrometry (GC‐MS) while the vanadium‐nickel and sulphur contents were determined by energy dispersive X‐ray spectrometry and inductively coupled plasma optical emission spectroscopy, respectively. Bulk and molecular characterizations indicated that the oils originated from a marine carbonate source rock containing oil‐prone Type II kerogen, consistent with generation by the La Luna Formation.Burial and thermal history modelling in the study area indicated that the La Luna source rock at wells X, Y and Z reached the oil window during the late Eocene, mid‐Eocene and early Paleocene respectively. 1D model results from the three wells showed that hydrocarbon generation began in the early Eocene. The transformation ratio of the La Luna Formation source rock in the modelled wells reached values of 35%, 98% and 100% by the end of the Eocene.In the Urdaneta West field, two different oil charges appear to have mixed in the Río Negro reservoir. Both oil charges were generated by the La Luna Formation source rock but at different times. A first charge of less mature oil occurred in the middle‐late Eocene (Phase 1 generation), and a second, more mature oil charge took place in the Miocene – Quaternary (Phase 2 generation). The kitchen area for the first oil charge is inferred to have been located to the north and NE of Urdaneta West; that for the second oil charge to the south of the field. This migration model is supported by observed variations in the geochemical compositions of the oil samples analysed from different wells in the Urdaneta West field.
Multimodal phenotypic labelling using drug‐induced sleep endoscopy, awake nasendoscopy and computational fluid dynamics for the prediction of mandibular advancement device treatment outcome: a prospective study
Authors: Van den Bossche Karlien , Op de Beeck Sara , Dieltjens Marijke , Verbruggen Annelies E. , Vroegop Anneclaire V. , Verbraecken Johan A. , Van de Heyning Paul H. , Braem Marc J. , Vanderveken Olivier M. 
Published: June 2022

SummaryMandibular advancement device (MAD) treatment outcome for obstructive sleep apnea (OSA) is variable and patient dependent. A global, clinically applicable predictive model is lacking. Our aim was to combine characteristics obtained during drug‐induced sleep endoscopy (DISE), awake nasendoscopy, and computed tomography scan‐based computational fluid dynamic (CFD) measurements in one multifactorial model, to explain MAD treatment outcome. A total of 100 patients with OSA were prospectively recruited and treated with a MAD at fixed 75% protrusion. In all, 72 underwent CFD analysis, DISE, and awake nasendoscopy at baseline in a blinded fashion and completed a 3‐month follow‐up polysomnography with a MAD. Treatment response was defined as a reduction in the apnea–hypopnea index (AHI) of ≥50% and deterioration as an increase of ≥10% during MAD treatment. To cope with missing data, multiple imputation with predictive mean matching was used. Multivariate logistic regression, adjusting for body mass index and baseline AHI, was used to combine all potential predictor variables. The strongest impact concerning odds ratios (ORs) was present for complete concentric palatal collapse (CCCp) during DISE on deterioration (OR 28.88, 95% confidence interval [CI] 1.18–704.35; p = 0.0391), followed by a C‐shape versus an oval shape of the soft palate during wakefulness (OR 8.54, 95% CI 1.09–67.23; p = 0.0416) and tongue base collapse during DISE on response (OR 3.29, 95% CI 1.02–10.64; p = 0.0464). Both logistic regression models exhibited excellent and fair predictive accuracy. Our findings suggest DISE to be the most robust examination associated with MAD treatment outcome, with tongue base collapse as a predictor for successful MAD treatment and CCCp as an adverse DISE phenotype.
240‐week entecavir maleate treatment in Chinese chronic hepatitis B predominantly genotype B or C
Authors: Xu Jing‐Hang , Fan Ya‐Nan , Yu Yan‐Yan , Si Chong‐Wen , Zeng Zheng , Xu Zhong‐Nan , Li Jun , Mao Qing , Zhang Da‐Zhi , Tang Hong , Sheng Ji‐Fang , Chen Xin‐Yue , Ning Qin , Shi Guang‐Feng , Xie Qing , Zhang Xi‐Quan , Dai Jun 
Published: June 2022

AbstractThis study aimed to evaluate the efficacy and safety of entecavir(ETV) versus ETV maleate in Chinese patients with chronic hepatitis B(CHB). This was a randomized, double‐blind, double‐dummy, controlled, multicentre study. Patients were randomly assigned to receive 48 weeks of treatment with 0.5 mg/day ETV (group A) or 0.5 mg/day ETV maleate (group B), then, all patients received treatment with 0.5 mg/day ETV maleate from week 49 onwards. Patients were regularly followed up. Serum hepatitis B virus (HBV) markers were detected. Adverse events (AE) were recorded. The primary endpoint was the decline in HBV DNA in each group at the end of treatment. Secondary endpoints included the rate of HBV DNA below the lower limit of detection (LLOD) (20 I U/ml) at the end of treatment, the rate of hepatitis B e antigen (HBeAg) loss, the rate of HBeAg seroconversion and serum alanine aminotransferase (ALT) normalization. One hundred and thirty‐seven (71 in group A) patients with HBeAg‐positive CHB and 46 (21 in group A) patients with HBeAg‐negative CHB completed the 240‐week treatment and follow‐up. Baseline characteristics were well balanced between the two groups. For the HBeAg‐positive CHB patients, the mean HBV DNA level had similarly decreased from baseline in both groups (A: by 6.67 log10 IU/ml vs. B: by 6.74 log10 IU/ml; p > .05) at Week 240. Patients who achieved undetectable levels of serum HBV DNA (<20 IU/ml) at Week 240 were similar between groups (A:91.55% vs. B:87.88%; p > .05). Both groups achieved similar HBeAg seroconversion rates at week 240 (A:26.98% vs. B:20.97%; p > .05). Both groups achieved similar normalization of ALT (A:87.32% vs. B:83.61%; p > .05) at Week 240 (p > .05). For the HBeAg‐negative CHB patients, the mean HBV DNA level had similarly decreased from baseline in both groups (A: by 6.05 log10 IU/ml vs. B: by 6.10 log10 IU/ml; p > .05) at Week 240. Patients who achieved undetectable levels of serum HBV DNA at Week 240 were similar between groups (A:100% vs. B:100%). Both groups achieved similar normalization rates (A:90.91% vs. B: 95.45%; p > .05) of ALT at Week 240 (p > .05). In conclusion, long‐term ETV maleate treatment was safe and efficient in Chinese CHB predominantly of genotype B or C.
Pharmacokinetics of maropitant citrate in Rhode Island Red chickens (Gallus gallus domesticus) following subcutaneous administration
Authors: Mones Alissa B. , Petritz Olivia A. , Knych Heather K. , Sadar Miranda J. , Thomson Andrea E. , Guzman David Sanchez‐Migallon 
Published: June 2022

AbstractMaropitant citrate is a synthetic neurokinin‐1 receptor antagonist and substance P inhibitor used for control of emesis in dogs in cats. Maropitant citrate is used empirically in birds, despite a lack of pharmacokinetic data in avian species. The objective of this study was to determine the pharmacokinetic profile of a single dose of maropitant citrate 1 and 2 mg/kg subcutaneously (SC) in eight Rhode Island Red hens (Gallus gallus domesticus). A crossover study design was used with 1‐week washout between trials. Blood samples were collected over 36 h after drug administration. Plasma concentrations were measured using liquid chromatography–tandem mass spectrometry and pharmacokinetic parameters were determined via non‐compartmental analysis. The mean maximum plasma concentration, time to maximum concentration, and elimination half‐life following 1 and 2 mg/kg SC were 915.6 ± 312.8 ng/ml and 1195.2 ± 320.2 ng/ml, 0.49 ± 0.21 h and 1.6 ± 2.6 h, and 8.47 ± 2.24 h and 8.58 ± 2.6 h, respectively. Pharmacokinetic data suggests doses of 1 or 2 mg/kg SC may be administered every 12–24 h to maintain above target plasma concentration similar to dogs (90 ng/ml). These data provide a basis for further investigation of maropitant citrate pharmacokinetics and pharmacodynamics in birds.
Spatial Bridge Locking Fixator versus Traditional Locking Plates in Treating AO/OTA 32‐A3.2 Fracture: Finite Element Analysis and Biomechanical Evaluation
Authors: Hu Jianwei , Peng Ye , Li Jiantao , Li Ming , Xiong Ying , Xiao Jiayu , Zhang Licheng , Tang Peifu 
Published: June 2022

ObjectiveTo compare the biomechanical behaviors of the spatial bridge locking fixator (SBLF), single locking plate (SP), and double locking plate (DP) for AO/OTA 32‐A3.2 fractures using finite element analysis and biomechanical tests.MethodsAxial loading of 700 N was conducted on the AO/OTA 32‐A3.2 model via finite element analysis. The von Mises stress and the interfragmentary movement (IFM) were comparatively analyzed in the three configurations above. On the mechanical tester, axial and torsional loading of 30 synthetic femurs (five specimens of each configuration for each test at random) was performed, and the interfragmentary movement, torsion angle, stiffness, and ultimate load were recorded and analyzed.ResultsThe finite element analysis (FEA) results showed that the von Mises stress of the spatial bridge locking fixator (SBLF) was lower than that of the single locking plate (SP) and higher than that of the double locking plate (DP). At 700 N, the axial IFMs were 0.15–0.38 mm (SBLF), 0.03–0.84 mm (SP), and 0.02–0.07 mm (DP). The biomechanical experiment indicated that the axial interfragmentary movements (IFMs) were 0.44 ± 0.23 mm (SBLF), 1.02 ± 0.40 mm (SP), and 0.07 ± 0.07 mm (DP) (p < 0.001). The axial IFM of the SBLF group had the highest probability (79.26%) of falling within the ideal range (0.2–0.8 mm), and the SP and DP groups had probabilities of 27.10% and 3.14%, respectively. The axial stiffness in the SBLF group (1586 ± 130 N/mm) was significantly lower than that in the DP group (10,264 ± 2671 N/mm) (p < 0.001) but greater than that in the SP group (725 ± 178 N/mm) (p = 0.396). The range of axial loads to ultimate failure was 3385–4527 N (SBLF), 3377–4664 N (SP), and 3780–4804 N (DP). The shear motion of the fracture end was 0.35 ± 0.14 mm (SBLF), 0.16 ± 0.10 mm (SP), and 0.08 ± 0.04 mm (DP) (p < 0.001). The torsional stiffness was 1.68 ± 0.14 Nm/degree (SBLF), 2.32 ± 0.29 Nm/degree (SP) (SBLF&SP, p < 0.001), and 3.53 ± 0.73 Nm/degree (DP) (SBLF&DP, p < 0.001).ConclusionsThe SBLF structure may exhibit a better biomechanical performance compared with the SP and DP in providing the best quantity and more symmetrical interfragmentary movement for AO/OTA 32‐A3.2 fractures.
Bone Hook Reduction Technique Combined with Lateral Parapatellar Arthrotomy for Periprosthetic Distal Femoral Fractures Following Total Knee Arthroplasty: A Technical Note
Authors: Shon Oog‐Jin , Kim Gi Beom , On Je Won 
Published: June 2022

ObjectiveTo report a bone hook reduction technique combined with lateral parapatellar arthrotomy for periprosthetic distal femoral fractures following total knee arthroplasty (TKA).MethodsFrom April 2012 to June 2018, a total of 31 knees who underwent this technique for the treatment of periprosthetic distal femoral fractures following TKA were retrospectively reviewed. Through a lateral parapatellar arthrotomy, the vastus lateralis fascia was dissected from the muscle belly to allow anteromedial mobilization of the muscles. With direct visualization of the posteriorly angulated distal fragment, a bone hook was placed on the anterior flange of the femoral component. The hook was then elevated to correct the posteriorly angulated and shortened distal fragment. The coronal and sagittal alignments of the distal segment with the femoral shaft were confirmed using fluoroscopic images, and internal fixation was performed using an anatomically pre‐contoured lateral locked plate. Once the overall length and sagittal plane alignment were restored, the plate was inserted via the previous articular approach. The plate was centered on the femur using anteroposterior and lateral fluoroscopy and then fixed.ResultsA total of 28 patients underwent internal fixation using the bone hook reduction technique combined with lateral parapatellar arthrotomy for the treatment of periprosthetic distal femoral fractures following TKA. The average age at operation was 70.9 years (range, 62–83 years), and the average follow‐up period was 17.5 months (range, 12–48.5 months). Fractures were classified as Su type I (13/28 [46.4%]), type II (11/28 [39.3%]), and type III (4/28 [14.3%]). Bone union was confirmed radiographically in all patients.ConclusionThe bone hook reduction technique is a simple and effective method to reduce the distal fragment in periprosthetic distal femoral fractures following TKA.
Use of Ilizarov Device to Gain Early Range of Motion in the Treatment of Pediatric Talus Body Fractures: A Series of Four Cases and Literature Review
Authors: Zhu WenTao , Chu ShuJuan , Li Qi , Rai Saroj , Liu WenQi , Tang Xin 
Published: June 2022

AbstractBackgroundTalus fractures are rare in children but can lead to severe outcomes if untreated. The Ilizarov external fixator has been used in the treatment of a variety of lower extremity pathologies. The purpose of this study was to investigate the clinical outcomes of talus body fractures treated with the Ilizarov external fixator.Case PresentationFour male pediatric patients (age range, 5–11 years) with talus body fractures who were treated by open reduction and internal fixation combined with Ilizarov external fixator between November 2015 and April 2016 were reviewed. Mean follow‐up period was 4 years (range, 4–5). Clinical outcome was evaluated using the clinical rating scale of the American Orthopaedic Foot and Ankle Society (AOFAS). All four patients achieved good to excellent results at the last follow‐up. None of the patients developed avascular necrosis. One patient developed automatic fusion of tibiotalar joint.ConclusionUse of the Ilizarov external fixator to gain early range of motion is a valuable option for treatment of talus body fractures in children.