bluuebunny/crossref_metadata_2025_split huggingface dataset:
Medical and biological studies of meat polycomponent products based on regional raw materials
Authors: Pasichnyi Vasyl , Tischenko Vasyl , Bozhko Nataliia , Marynin Andriy , Moskaluyk Oksana , Geredchuk Alina 
Published: October 2022

The article is devoted to the study of the medical and biological safety of consumption of multicomponent meat-rich semi-finished products and semi-smoked sausage. When creating functional products, the approach of multi-ingredient modeling of the future product is applied according to the principle of adding nutritional and biological value. The multi-component nature of the constituent ingredients with different chemical composition makes it possible to obtain products with specified characteristics. There is a problem of ensuring the food safety of the developed product during production, storage and consumption of finished products.
The purpose of the study was to study the effect of the developed meat-containing polycomponent semi-finished products and semi-smoked sausage on the growth dynamics and the level of metabolic processes of experimental rats when they are introduced into the standard diets of animals in the amount of 30 %. The work was performed on 36 white non-linear rats weighing 160–200 g.
Medical and biological studies of multicomponent meat-containing products have shown their absence of a negative effect on the growth and development of animals, the level of metabolic processes during the growth period.
It has been established, that feeding rats with meat-rich polycomponent products in the amount of 30 % of the standard diet increases the live weight of rats by 33–38 % within 3 weeks. Anatomopathological changes in the internal organs of the experimental rats were not detected.
The inclusion of meat-rich multicomponent products in the diet of rats promotes the strengthening of hematological processes in the body, increases the concentration of erythrocytes in the blood to 8.01±0.11·1012/l, which is 12.7 % higher, compared to the control animals, increases the hemoglobin content in erythrocytes by 45.83–58.33 % higher, compared to the control animals.
It has been proven, that the consumption of meat-containing multicomponent products by rats leads to an increase in the concentration of total protein by 4.79 %, hemoglobin by 42.12 %, and creatinine by 19.68 %. Enhanced protein synthesis due to intensification of catabolism leads to an increase in bilirubin concentration by 19.12–21.97 % compared to the control rats
Determination of the species structure of phytocenoses of the natural age oak forest of the Dendrological Park "Olexandria" of the National Academy of Sciences of Ukraine
Authors: Boiko Nataliia , Dragan Nina , Doiko Nataliia , Pydorych Yuriy , Krivdyuk Larisa 
Published: October 2022

The modern species composition of woody plants of the centuries-old oak grove of the State Dendrological Park "Olexandria" of the National Academy of Sciences of Ukraine was studied.
The work was carried out as part of the study of the dynamics of stands of oak phytocenoses under conditions of anthropogenic load, technogenic pollution and weather fluctuations.
It has been established that the oak grove of the arboretum with an area of 446 hectares out of 2000 oaks is a complex multi-species planting. The floristic core of the oak forest consists of the main forest-forming species characteristic of the Right-Bank Forest-Steppe of Ukraine: Quercus robur, Acer platanoides L. and Acer campestre L., Fraxinus excelsior L., Tilia cordata Mill., Carpinus betulus L., Ulmus scabra Mill. The role of the dominant species and edificator was retained by Quercus robur. In the worst growing conditions, monodominant stands are formed. In more favorable ecotopes in the upper tree layer, the participation of Quercus robur is 70–100 %, and its codominant satellites (Acer platanoides, Tilia cordata, Fraxinus excelsior) are 10 % each. All oak forest phytocenoses have a well-developed multi-species second tree layer: Acer platanoides (15–80 % in various phytocenoses), Acer campestre (10–30 %), Tilia cordata (10–60 %), in some phytocenoses Fraxinus excelsior (10–20 %), Ulmus scabra (10–20 %), Carpinus betulus (30 %).
The group of asectators is diverse and is represented by small species, local and adventive species, introducers, including invasive species.
A characteristic feature of the oak forest is its exorbitant crushing, which, along with the massive introduction of introduced species, caused a powerful ecotonization of the oak forest and formed the cells of the greatest mortality of Quercus robur.
The conducted studies establish the modern species composition of the oak forest and are the basis for the study of succession processes in oak forest phytocenoses.
Microbial contamination and quantitative microbial risk assessment of high-density polyethylene (HDPE) film sachet drinking water in Ghana
Authors: Angnunavuri Prosper Naah , Attiogbe Francis , Mensah Bismark 
Published: October 2022

AbstractThe present research estimated the impact of storage on the microbial quality of high-density polyethylene drinking water. Samples were taken from two popular companies in Greater Accra using a two-sided exact test in SAS JMP to estimate the sample size. The samples were stored across three temperature profiles at 8 °C, 30 °C (average room temperature), and 40 °C (average outdoor temperature) for 28 days. The samples were examined using standard microbiological methods for heterotrophic plate counts (HPCs), faecal coliforms, and Escherichia coli. The data were described and regressed with Microsoft Excel, Argo 4.3.1, and SAS JMP software. The results demonstrated increasing deterioration of the water samples for all microbial indices at all temperatures with increasing storage duration. The highest HPC, faecal coliforms, and E. coli were 1,312; 622; and 252 cfu/100 mL, respectively, all at 40 °C. The daily risk of infection due to E. coli O157:H7 was 5.22 × 10−5 infections per child per day for children under 5 years, and 1.6 × 10−4 attacks per adult per day, compared to the upper limit of 1.0 × 10−6. These results are higher than recommended exposures, and interventions along the sachet drinking water value chain are needed to protect public health.
Solution structure of the wild-type HMGI a box by 1 h and 15n NMR and molecular modelling
Authors: Sodano P. , Ohyama Y. , Locker D. , Bianchi M. E. , Leng M. , Vovelle F. , Ptak M. 
Published: October 2022

Abstract:
The solution structure of the wild-type A box of rat HMG1 has been determined by
heteronuclear three-dimensional NMR and molecular modelling. This structure adopts a "L" shape
structure globaly similar to that described for a mutant A box and other HMG boxes. However the
wild-type A box contains two Cys residues which could form intramolecular bridge in an
oxidative environment. This should be into competition with a contribution of the thiol groups in
the binding ofHMG proteins to cisplatin-modified DNA.
The primary structure of the J3 subunit of desulfovjbrio desulfuricans (atcc 27774) [nife] hydrogenase
Authors: Calvetel Juan J. , Francol Ricardo , Thole Hubert H. , Raida Manfred , Moura Isabel , Moura Jose J. G. 
Published: October 2022

Abstract:
The periplasmic [NiFe] hydrogenase isolated from Desulfovibrio (D.) desulfuricans (ATCC 27774) is a
heterodimer of a 28 kDa (13) and a 60 kDa (a) subunit. Here we report the complete amino acid sequence of the
small (13) polypeptide chain determined by Edman degradation of proteolytic fragments. Electrospray-ionization
mass spectrometry of the native protein confirmed the sequencing results. The sequence is compared with that
of D. gigas [NiFe] hydrogenase whose three-dimensional structure has been recently published.
Rapid and efficient purification of rat brain dynamin using an affinity column of the carboxy-terminal sh3 domain of grb2
Authors: Roques Bernard P. , Montiel Jose-Luis , Cussac Didier , Cornille Fabrice , Vidal Michel , Garbay Christiane 
Published: October 2022

Abstract:
The carboxy-terminal SH3 domain of Grb2 coupled to sepharose gel was used to purify rat brain dynamin I. The affinity chromatography yielded two dynamin-rich fractions eluted with high salt plus detergent and with low concentration of guanidinium-HCl, respectively. Both pools were purified to homogeneity using an ATP/agarose column and were shown by Far-Western blots and surface plasmon resonance (SPR) to have similar interactions with Grb2. The yield of this rapid purification procedure is significantly increased as compared to previously reported protocols.
Using capillary electrophoresis to trace hydrolysis	of the	reactive site	peptide bond of trypsin inhibitors
Authors: Rozycki Jan , Mucha Piotr , Rolka Krzysztof , Rekowski Piotr 
Published: October 2022

Abstract:
Application of capillary electrophoresis for determination of the reactive site peptide bond hydrolysis in serine protease protein inhibitors by bovine ,B-trypsin is reported in this paper. The advantage of this technique for such investigations has been underlined.
Use of tnbs colorimetric assay to, analyze viral protease inhibitors
Authors: Wang Q. May , Johnson Robert B. 
Published: October 2022

Abstract:
Peptides derived from the rhinovirus-14 3C native cleavage sites have been synthesized and evaluated as substrates for this protease. Hydrolysis of the N-terminally acetylated peptides by 3C generates primary amino groups which react with 2,4,6-trinitrobenzenesulfonic acid at an alkaline pH. We have optimized the assay by selecting a sensitive monitoring wavelength to detect the resulting orange-colored trinitrophenyl products. Rhinovirus-14 3C cleaves the 3B/3C peptide with a Km value of0.75 mM as determined by this method. Assay characterization and its use in protease inhibitor study have been performed.
Mutant of hiv-1 protease with new specific properties
Authors: N.S. Andreeva , N.I. Dergousova , F. Leonova Yu , A.A.I Zinchenko , L.D. Rumsh 
Published: October 2022

Abstract:
An extensive net of hydrogen bonds including the active carboxyls and Tyr residue locating in the so-called 'flap' in pepsin-like enzymes was planned to be restored in molecule of HIV-1 protease. For this purpose, 5 mutations and 2 deletions were introduced simultaneously in the enzyme. Some of the residues subjected to mutagenesis are known to be changed during formation of drug resistant HIV-1 protease. The obtained mutant was fully active, but had an altered specificity and pH optimum.
The influence of n-terminal fusion partners on the production and folding of recombinant
human procathepsin E
Authors: Tatnell Peter J. , Kay John 
Published: October 2022

Abstract:
The production of human procathepsin E in Escherichia coli was optimised by expression in the form of N­ terminal tagged fusion proteins using several T7-RNA polymerase dependent pET-vectors. The nature of the N-terminal fusion partner did not significantly influence the amount of the precusor that accumulated. However, the yield of active, mature cathepsin E that was recovered after refolding varied by as much as ten-fold.
Assay of lumbrokinase with a chromophoric substrate
Authors: He Rong-Qiao , Zhou Jing , Fan Rong , Wu Chen 
Published: October 2022

Abstract:
A new method to assay lumbrokinase with a chromophoric substrate was introduced. Compared with the fibrin plate method, the chromophoric assay is rapid, convenient, accurate and repeatable. The activity of lumbrokinase can be defined as the international unit directly with this method.
Parental Autonomy Support and Psychological Well-Being in University Students During the COVID-19 Pandemic: The Role of Autonomy Satisfaction
Authors: Akram Ananda Rainy , Abidin Fitri Ariyanti , Lubis Fitriani Yustikasari 
Published: October 2022

Background:University students, as emerging adults, have autonomy as one of their central developmental tasks. Parents need to provide an autonomy-supportive environment to encourage students to act based on their volition, benefiting their psychological well-being. This study aims to explore the effect of parental autonomy support on psychological well-being by the mediation of autonomy satisfaction.Methods:227 university students in Indonesia (21.6% male, 78.4% female) aged 18-24 years old (M = 20.73; SD = 0.93) participated in the study and completed an online survey. We used three validated Indonesian version questionnaires: Parental Autonomy Support, Basic Psychological Needs Satisfaction and Frustration, and Psychological Well-Being.Results:The result shows that autonomy satisfaction fully mediated the relationship between parental autonomy support and psychological well-being.Conclusion:This finding supported the self-determination theory framework and implied the importance of parental autonomy support in enhancing university students' psychological well-being, especially in pandemic situations.
Hepatitis B virus prevalence in three university students in Timor-Leste
Authors: Silva Hedriketa Da 
Published: October 2022

Hepatitis B is a deadly and contagious disease that knows no age,both children, adults and the elderly. Timor-Leste is one of the newly independent countries within 20 years, however, in terms of infectious diseases, one of which is hepatitis B, in this youngest country it has been categorized with a high endemicity of 8.5%- 12.4% (Silva, 2017). Thus, it is necessary to anticipate so that the prevalence of hepatitis B does not continue to rise, and immediately take early prevention for the younger generation in the country of Timor-Leste, therefore one way is to carry out early detection of hepatitis B in students with the aim of knowing the presence or absence of infection. hepatitis B virus in a person's body. Thus, it can provide recommendations to the government to prevent it by vaccinating all future generations. The main reason for early detection of medical and health science students at the three universities is to prevent horizontal transmission of the hepatitis B virus in Timor-Leste. This study aims to determine the prevalence of hepatitis B virus infection and risk factors for hepatitis B virus infection in students of the Faculty of Medicine and Health Sciences from three universities in Timor-Leste.
Clinical case of Norwegian scabies in a patient with HIV infection
Authors: Bulatova A. Kh. , Khaertynov Kh. S. , Idrisov I. G. , Gilfanov N. M. , Kuznetsov A. S. 
Published: October 2022

Norwegian scabies is a rare variant of scabies, which has clinical differences from the classical variant, which can cause diagnostic errors. Risk factors for developing the disease are various immunodeficiency states (HIV infection, malignant neoplasms, leprosy). A clinical case of the development of Norwegian scabies in a 22-year-old patient with HIV-infection stage 4B, with a level of CD4-lymphocytes in the blood of 8 cells/µl, is presented. The patient was hospitalized with complaints of weakness, skin rashes, itching and fever up to 40°C. On examination, there was dryness of the skin with erythematous areas on the arms, legs, and torso, as well as massive dirty-gray crusts on the skin of the scalp, torso, palms, elbows, dorsal surface of the feet. Purulent crusts were visualized on the elbows (Ardy’s symptom).Treatment of scabies was carried out by treating the skin with a solution of benzyl benzoate. Against the background of the therapy, there was a positive trend in the form of the disappearance of skin itching, unpleasant odor, and complete disappearance of crusts on the 7th day of treatment.
Clinical cases of pneumocystis pneumonia in patients with HIV infection and underlying COVID-19
Authors: Ilieva K. , Karnaukhova E. Yu. , Pobegalova O. E. , Lioznov D. A. 
Published: October 2022

During the COVID-19 pandemic, additional difficulties have emerged in the differential diagnosis of interstitial pulmonary abnormalities, especially in patients with HIV infection, in whom this kind of injury can be caused by a wide range of pathogens, including opportunistic diseases. The high probability of an adverse outcome of pulmonary disease in patients with severe immunodeficiency requires an urgent choice of effective therapy.The article describes clinical cases of pneumocystis pneumonia in two COVID-19 patients with newly diagnosed HIV infection, illustrating the difficulties of differential diagnosis in these conditions.
Edukasi dan penyuluhan potensi limbah jerami padi menjadi pupuk organik bagi pertanian di Desa Sidomulyo
Authors: Pratama Brahma Putra 
Published: October 2022

Desa Sidomulyo merupakan satu dari dua belas desa yang ada di Kecamatan Pengasih, Kabupaten Kulon Progo, Daerah Istimewa Yogyakarta. Desa ini memiliki potensi desa dari berbagai sektor seperti pertanian, peternakan, pariwisata, dan usaha kecil. Produksi tanaman pertaniannya antara lain padi, jagung, pohon, ketela rambat, kacang tanah, kacang kedelai, dan kacang hijau. Desa Sidomulyo sendiri menggunakan pupuk kandang dan pupuk kimia, padahal limbah jerami padi dari hasil panen biasanya hanya dibakar oleh para petani setempat. Maka dari itu melalui program Kuliah Kerja Nyata yang diadakan oleh Universitas Atma Jaya Yogyakarta angkatan 80 ini, kami menyusun suatu program yaitu “Edukasi dan penyuluhan potensi limbah jerami padi menjadi pupuk organik bagi pertanian di Desa Sidomulyo”.
PENGEMBANGAN KURSUS ONLINE INTERAKTIF DENGAN SISTEM E-LEARNING DAN GAMIFIKASI
Authors: Susanto Dwi , Muhammad Aliv Faizal , Sa'dyah Halimatus , Nabhan Salim , Sumarsono Irwan 
Published: October 2022

Abstrak: Sejak wabah pandemi Cover-19, permintaan untuk menyediakan sumber daya pendidikan untuk pembelajaran jarak jauh terus meningkat. Karena keterlibatan belajar dan partisipasi aktif siswa menjadi beberapa aspek mendasar dari proses pembelajaran online mandiri yang efektif, aspek interaktivitas dan motivasi menjadi penting. Gamifikasi disarankan untuk diterapkan dalam pembelajaran online asinkron. Poin, penghargaan, catatan prestasi, dan aspek gamifikasi lainnya telah dilaporkan efektif dalam meningkatkan keterlibatan siswa dalam pembelajaran. Kelas bahasa Inggris akan menjadi contoh konten pembelajaran di sistem E-learning yang didukung gamification ini. Pembelajaran online ini dilengkapi dengan berbagai kuis yaitu pilihan ganda, drag and drop, isian, teka-teki silang, serta kuis dikte berbasis pengenalan suara. Aktivitas siswa dicatat sebagai poin. Hasil implementasi ini menunjukkan bahwa model tersebut dapat diterapkan untuk mata pelajaran lain untuk dikonversi atau disediakan dalam pembelajaran online, khususnya untuk gaya belajar mandiri melalui model e-learning asinkron.Keyword: Pembelajaran Bahasa Inggris, Wordpress, H5P, Gamifikasi, E-Learning Abstract: Since the Cover-19 pandemic outbreak, the demand for providing educational resources to distance learning keeps increasing. As students' learning engagement and active participation become some of fundamental aspects of effective autonomous online learning process, interactivity and motivational aspects become of necessity. Gamification is suggested to be implemented in an asynchronous online course. Points, rewards, and achievement records, and other gamification aspects have been reported effective in increasing students' engagement in the learning. An English course was to be the sample course content made available in an online course in which this gamification-supported E-learning system is implemented. The online course comes with types of quizzes namely multiple choice, drag and drop, fill in the blank, crossword puzzle, as well as speech recognition based dictation quiz. The students' activities are recorded as points awarded to the students' user page. The result of this implementation shows that the model can be implemented for other learning subject to be converted or made available in an online learning course, especially for autonomous learning style through asynchronous e-learning model.Keywords: Teaching English, Wordpress, H5P, Gamification, E-Learning
PENGARUH STRATEGI PEMBELAJARAN KOOPERATIF DAN KEPERCAYAAN DIRI TERHADAP HASIL BELAJAR IPA SISWA KELAS IV SD
Authors: Sabar Ahmad , Napitupulu Efendi , Listyarini Sri 
Published: October 2022

Abstrak: Proses pembelajaran yang dilakukan guru masih belum mampu mengaktifkan siswa secara optimal dalam belajar. Penelitian bertujuan untuk menganalisis pengaruh: (1) strategi pembelajaran kooperatif terhadap hasil belajar siswa, (2) kepercayaan diri terhadap hasil belajar siswa, dan (3) interaksi strategi pembelajaran dan kepercayaan diri terhadap hasil belajar IPA siswa. Sampel penelitian sebanyak 2 kelas. Intrumen penelitian berupa angket dan tes hasil belajar IPA yang telah memenuhi kriteria valid dan reliabel. Data dianalisis menggunakan teknik ANAVA dua jalur dengan desain faktorial 2×2. Hasil penelitian menunjukkkan: hasil belajar siswa yang diajarkan pembelajaran kooperatif tipe group investigation (77,14) lebih tinggi dibandingkan yang diajarkan tipe picture and picture (70,27); hasil belajar siswa dengan kepercayaan diri tinggi (77,08) lebih tinggi dibandingkan siswa dengan kepercayaan diri rendah (70,75); dan tidak terdapat interaksi strategi pembelajaran kooperatif dan tingkat kepercayaan diri. Kesimpulan penelitian: (1) terdapat pengaruh strategi pembelajaran kooperatif terhadap hasil belajar siswa, (2) terdapat pengaruh kepercayaan diri terhadap hasil belajar siswa, dan (3) tidak terdapat interaksi antara strategi pembelajaran kooperatif dan kepercayaan diri terhadap hasil belajar IPA siswa.Kata kunci: group investigation, picture and picture, hasil belajar IPAAbstract: The learning process carried out by the teacher is still not able to activate students optimally in learning. The research aims to analyze the effect of: (1) cooperative learning strategies on student learning outcomes, (2) confidence in student learning outcomes, and (3) the interaction of learning strategies and self-confidence on students' science learning outcomes. The research sample was 2 classes. Research instruments in the form of questionnaires and tests of science learning outcomes that have met the valid and reliable criteria. Data were analyzed using two-way ANOVA technique with a 2×2 factorial design. The results showed: the learning outcomes of students who were taught cooperative learning with the group investigation type (77.14) were higher than those taught by the picture and picture type (70.27); student learning outcomes with high self-confidence (77.08) are higher than students with low self-confidence (70.75); and there is no interaction between cooperative learning strategies and the level of self-confidence. Research conclusions: (1) there is an effect of cooperative learning strategies on student learning outcomes, (2) there is an effect of self-confidence on student learning outcomes, and (3) there is no interaction between cooperative learning strategies and self-confidence on students' science learning outcomes. Keywords: group investigation, picture and picture, science learning outcomes