bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical utility of reticulocyte haemoglobin in the assessment of iron deficiency and iron deficiency anaemia in the paediatric population
Authors: Perchard Malaika , Barbaro Pasquale , Rane Mark , Norman Andrew 
Published: November 2022

AimReticulocyte haemoglobin (Ret‐He) is a useful marker in the assessment of iron stores in adult and paediatric patients. It is currently not utilised in Pathology Queensland. The objective of this study is to verify Ret‐He in our Pathology Queensland laboratory and assess the clinical utility in the assessment of iron deficiency (ID) and iron deficiency anaemia (IDA) in paediatric patients.MethodsSamples from patients aged <18 years sent to the Pathology Queensland laboratory that had paired full blood count and iron studies were included in this study. A minimum of 120 samples were required for verification of testing requirements and a minimum of 30 samples per age range were required for confirmation of published age‐related reference intervals.ResultsPublished Ret‐He reference intervals were confirmed for stated age ranges in normal (non‐ID) patients. Ret‐He below the reference range for age demonstrated a good correlation with ID and IDA.ConclusionsRet‐He is a useful marker in the assessment of ID and IDA in a paediatric population. It is not affected by acute or chronic inflammation. Ret‐He is sensitive and specific (86% and 92%) for the diagnosis of ID.
Medicaid expansion and access to naloxone in metropolitan and nonmetropolitan areas
Authors: Cheng Yue , Freeman Patricia R. , Slade Emily , Sohn Minji , Talbert Jeffery C. , Delcher Chris 
Published: November 2022

AbstractPurposeThe opioid crisis remains a major public health concern in the United States. Naloxone is used to reverse opioid overdoses. This study examined Medicaid expansion on naloxone prescriptions in retail pharmacies in metropolitan (metro) and nonmetropolitan (nonmetro) areas (2011‐2017).MethodsWe used population average models to evaluate the association of Medicaid expansion at the state level on the number of naloxone prescriptions dispensed and the percentage paid by Medicaid, including adjustment for opioid‐related and state‐level policy covariates. Difference‐in‐difference modeling was performed as a sensitivity analysis.FindingsStates that expanded Medicaid had higher unadjusted naloxone dispensing rates and Medicaid‐paid percentage of naloxone in metro and nonmetro areas. Medicaid expansion was not associated with the number of naloxone dispensed in either metro (adjusted rate ratio (ARR) = 1.26, 95% CI: [0.80, 1.97]) or nonmetro (ARR = 0.67, 95% CI: [0.37, 1.19]) areas after covariate adjustment. In metro areas, Medicaid expansion was associated with a significant increase of 3.86 percentage points (95% CI: [0.09, 7.63]) in the Medicaid‐paid percentage of naloxone dispensing compared to nonexpansion states, but this association was not significant in nonmetro areas. There was also a significant time by Medicaid expansion interaction on the Medicaid‐paid percentage of naloxone dispensed (metro: estimate = 0.74, 95% CI: [0.36, 1.12]; nonmetro: estimate = 0.68, 95% CI: [0.17, 1.18]).ConclusionsMedicaid expansion increased naloxone access by increasing the Medicaid‐paid percentage of naloxone prescriptions in metro areas. States with Medicaid expansion had a faster rate of increase in the Medicaid‐paid percentage of naloxone than states without Medicaid expansion in nonmetro areas.
Microbiome assembly and maintenance across the lifespan of bumble bee workers
Authors: Hammer Tobin J. , Easton‐Calabria August , Moran Nancy A. 
Published: November 2022

AbstractHow a host's microbiome changes over its lifespan can influence development and ageing. As these temporal patterns have only been described in detail for a handful of hosts, an important next step is to compare microbiome succession more broadly and investigate why it varies. Here we characterize the temporal dynamics and stability of the bumble bee worker gut microbiome. Bumble bees have simple and host‐specific gut microbiomes, and their microbial dynamics may influence health and pollination services. We used 16S rRNA gene sequencing, quantitative PCR and metagenomics to characterize gut microbiomes over the lifespan of Bombus impatiens workers. We also sequenced gut transcriptomes to examine host factors that may control the microbiome. At the community level, microbiome assembly is highly predictable and similar to patterns of primary succession observed in the human gut. However, at the strain level, partitioning of bacterial variants among colonies suggests stochastic colonization events similar to those observed in flies and nematodes. We also find strong differences in temporal dynamics among symbiont species, suggesting ecological differences among microbiome members in colonization and persistence. Finally, we show that both the gut microbiome and host transcriptome—including expression of key immunity genes—stabilize, as opposed to senesce, with age. We suggest that in highly social groups such as bumble bees, maintenance of both microbiomes and immunity contribute to inclusive fitness, and thus remain under selection even in old age. Our findings provide a foundation for exploring the mechanisms and functional outcomes of bee microbiome succession.
Transcriptional analysis of the mfa‐cluster genes in Porphyromonas gingivalis strains with one and two mfa5 genes
Authors: Fujita Mari , Chiu Chen‐Hsuan , Nagano Keiji 
Published: November 2022

AbstractThe Porphyromonas gingivalis Mfa1 fimbria is composed of the Mfa1 to Mfa5 proteins, encoded by the mfa1 to mfa5 genes, respectively, which are tandemly arranged on chromosomes. A recent study discovered that many P. gingivalis strains possess two mfa5 genes (called herein mfa5‐1 and mfa5‐2), which are also in tandem. This study examined the transcriptional unit and activity of mfa‐cluster genes in strains with one (the ATCC 33277 and TDC60 strains) and two (the HG66 and A7436 strains) mfa5 genes. Complementary DNA was prepared from the total RNA extracted from the bacterial cells in the logarithmic growth phase using a random primer. PCR analysis for the intergenic regions from mfa1 to mfa5 or mfa5‐2 showed that mfa1 to mfa5 or mfa5‐2 formed a polycistronic gene cluster. Quantitative real‐time PCR showed that the mfa1 transcription was 5–10 times higher than that of mfa2 in all the strains. However, mfa2 to mfa5 mostly showed a comparable expression. Both mfa5 genes were comparably transcribed in HG66 and A7436 strains. The transcriptional levels were almost consistent with the respective protein expression levels. In silico analysis identified a transcriptional terminator structure in the intergenic region between mfa1 and mfa2 that was probably responsible for the decreased transcription rate of mfa2 and the downstream genes.
Regional oxygen saturation measured by two different oximetry monitors in infants and children undergoing living donor liver transplantation with bilirubin measurements: A prospective observational study
Authors: Magasich‐Airola Natalia Patricia , Momeni Mona , Sanchez Torres Cristel , De Magnée Catherine , Tambucci Roberto , Reding Raymond , Pirotte Thierry 
Published: November 2022

AbstractBackgroundTissue oximetry devices use wavelengths in the 680–870 nm range to separate between oxygenated/deoxygenated hemoglobin. Conjugated bilirubin has an absorption peak at 730 nm.AimsWe hypothesized that ForeSight Elite using 5 wavelengths reduces interference from bilirubin and shows higher regional tissue oxygen saturation (rSO2) than INVOS 5100C incorporating 2 wavelengths.MethodsInfants and children undergoing living donor liver transplantation were included between March 2019 and September 2020. Cerebral and somatic rSO2 were measured, and real‐time simultaneous data were collected. Additionally, measurements were collected at (1) baseline, (2) beginning of dissection phase, (3) beginning of anhepatic phase, (4) reperfusion phase, and (5) skin closure. Bilirubin level was available at baseline and at reperfusion. Hyperbilirubinemia was defined as bilirubin level ≥1.0 mg/dl.ResultsThirty‐three patients with median age of 27 months and median weight of 12 kg were included. Baseline bilirubin levels were higher compared to values at reperfusion (p = .021). A linear mixed effects model considering bilirubin as fixed and patient as random effect showed that there was a statistically significant difference in cerebral rSO2 readings in function of time (p = .031), device (p < .001), and bilirubin concentrations (p = .007) but not for hemoglobin (p = .347), SpO2 (p = .882), and arterial partial pressure of CO2 (PaCO2) (p = .146). The model showed that there was a statistically significant difference in somatic rSO2 readings in function of device (p < .001) and bilirubin concentrations (p = .023) but not for time (p = .074), hemoglobin (p = .954), SpO2 (p = .108), and PaCO2 (p = .775). Bland–Altman plot analyzing cerebral and somatic rSO2 between both devices showed respectively a mean absolute bias and 95% limits of agreement of 21.73% (−10.21 to 53.67) and 19.52% (−29.51 to 68.54).ConclusionsOximetry devices emitting light at >2 wavelengths may overcome interference from hyperbilirubinemia providing higher rSO2 readings.
Development of a 135K SNP genotyping array for Actinidia arguta and its applications for genetic mapping and QTL analysis in kiwifruit
Authors: Wang Ran , Xing Siyuan , Bourke Peter M. , Qi Xiuquan , Lin Miaomiao , Esselink Danny , Arens Paul , Voorrips Roeland E. , Visser Richard G.F. , Sun Leiming , Zhong Yunpeng , Gu Hong , Li Yukuo , Li Sikai , Maliepaard Chris , Fang Jinbao 
Published: November 2022

SummaryKiwifruit (Actinidia spp) is a woody, perennial and deciduous vine. In this genus, there are multiple ploidy levels but the main cultivated cultivars are polyploid. Despite the availability of many genomic resources in kiwifruit, SNP genotyping is still a challenge given these different levels of polyploidy. Recent advances in SNP array technologies have offered a high‐throughput genotyping platform for genome‐wide DNA polymorphisms. In this study, we developed a high‐density SNP genotyping array to facilitate genetic studies and breeding applications in kiwifruit. SNP discovery was performed by genome‐wide DNA sequencing of 40 kiwifruit genotypes. The identified SNPs were stringently filtered for sequence quality, predicted conversion performance and distribution over the available Actinidia chinensis genome. A total of 134 729 unique SNPs were put on the array. The array was evaluated by genotyping 400 kiwifruit individuals. We performed a multidimensional scaling analysis to assess the diversity of kiwifruit germplasm, showing that the array was effective to distinguish kiwifruit accessions. Using a tetraploid F1 population, we constructed an integrated linkage map covering 3060.9 cM across 29 linkage groups and performed QTL analysis for the sex locus that has been identified on Linkage Group 3 (LG3) in Actinidia arguta. Finally, our dataset presented evidence of tetrasomic inheritance with partial preferential pairing in A. arguta. In conclusion, we developed and evaluated a 135K SNP genotyping array for kiwifruit. It has the advantage of a comprehensive design that can be an effective tool in genetic studies and breeding applications in this high‐value crop.
Disseminated Bisifusarium infection following toxic epidermal necrolysis in a child with B‐cell acute lymphoblastic leukemia
Authors: Brent Geoffrey , Abdul‐Wahab Alya , Borman Andrew M. , Ferguson Leila , Ferreras‐Antolin Laura , Ho Bernard , Johnson Elizabeth M. , Mashhoudi Yasaman , van Rijswijk Elsje , Wijesuriya Nilukshi , Mansoor Nazish 
Published: November 2022

AbstractFusarium is a polyphyletic genus of plant pathogens, members of which can cause opportunistic human infections with varying superficial and systemic presentations, including disseminated infections which typically occur in immunocompromised patients and have a poor prognosis. Treatment is challenging due to intrinsic resistance to many antifungal agents, and antifungal susceptibility testing is therefore essential. Early suspicion, isolation of the organism, and prompt initiation of management are crucial to improving survival. We present a case of disseminated Bisifusarium infection following toxic epidermal necrolysis in a child with B‐cell acute lymphoblastic leukemia, successfully treated with liposomal amphotericin B, voriconazole, flucytosine, and terbinafine.
Wittgenstein on Miscalculation and the Foundations of Mathematics
Authors: Wheeler Samuel J. 
Published: November 2022

AbstractIn Remarks on the Foundations of Mathematics, Wittgenstein notes that he has ‘not yet made the role of miscalculating clear’ and that ‘the role of the proposition: “I must have miscalculated”…is really the key to an understanding of the “foundations” of mathematics.’ In this paper, I hope to get clear on how this is the case. First, I will explain Wittgenstein's understanding of a ‘foundation’ for mathematics. Then, by showing how the proposition ‘I must have miscalculated’ differentiates mathematics from the physical sciences, we will see how this proposition is the key to understanding the foundations of mathematics.
Role of the OTUB1/IRF7/NOX4 axis in oxidative stress injury and inflammatory responses in mice with Parkinson's disease
Authors: Xing Xiaolian , Xu Fei , Wang Yu , Liu Hongwei 
Published: November 2022

AbstractBackgroundParkinson's disease (PD) is one of the most devastating neurodegenerative disorders and is associated with oxidative stress injury (OSI) and inflammatory responses. This study sought to investigate the mechanism of ovarian tumour domain‐containing ubiquitin aldehyde binding 1 (OTUB1) in OSI and inflammatory responses in PD, providing a theoretical foundation for PD treatment.MethodsThe PD mouse model was established by an intraperitoneal injection of 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine, followed by behavioural tests, observation of brain pathological changes, and quantification of inflammatory (TNF‐α, IL‐1β, and IL‐10) and OS (ROS, SOD, and MDA) factors. Next, the expression levels of OTUB1, interferon regulatory factor 7 (IRF7), and NADPH oxidase 4 (NOX4) levels were determined by real‐time quantitative polymerase chain reaction and western blot assay, the binding of OTUB1 to IRF7 was analysed by co‐immunoprecipitation, and the ubiquitination level of IRF7 and the enrichment and binding of IRF7 and the NOX4 promoter were measured by chromatin immunoprecipitation and dual‐luciferase assays. Afterwards, rescue experiments were performed with IRF7 or NOX4 overexpression in OTUB1 knockout PD mice.ResultsOTUB1 was upregulated in brain tissues of PD mice. Inhibition of OTUB1 alleviated PD progression, OSI, and inflammatory responses. OTUB1 stabilized IRF7 through deubiquitination, and IRF7 bound to the NOX4 promoter to promote NOX4 expression. IRF7 or NOX4 overexpression reversed the effects of silencing OTUB1 on OSI and inflammatory responses in PD mice.ConclusionOTUB1‐mediated deubiquitination stabilized IRF7 and upregulated NOX4 expression, thereby promoting OSI and inflammatory responses in PD mice.
Windowed Green function method for wave scattering by periodic arrays of 2D obstacles
Authors: Strauszer‐Caussade Thomas , Faria Luiz M. , Fernandez‐Lado Agustín , Pérez‐Arancibia Carlos 
Published: November 2022

AbstractThis paper introduces a novel boundary integral equation (BIE) method for the numerical solution of problems of planewave scattering by periodic line arrays of two‐dimensional penetrable obstacles. Our approach is built upon a direct BIE formulation that leverages the simplicity of the free‐space Green function but in turn entails evaluation of integrals over the unit‐cell boundaries. Such integrals are here treated via the window Green function method. The windowing approximation together with a finite‐rank operator correction—used to properly impose the Rayleigh radiation condition—yield a robust second‐kind BIE that produces superalgebraically convergent solutions throughout the spectrum, including at the challenging Rayleigh–Wood anomalies. The corrected windowed BIE can be discretized by means of off‐the‐shelf Nyström and boundary element methods, and it leads to linear systems suitable for iterative linear algebra solvers as well as standard fast matrix–vector product algorithms. A variety of numerical examples demonstrate the accuracy and robustness of the proposed methodology.
The body maledict: Understanding the method of standpoint phenomenology through the work of Frantz Fanon
Authors: Ward Katherine 
Published: November 2022

AbstractIn this article, I examine the phenomenological methodology at work in Fanon's revision of the body schema. I argue that he implicitly utilizes a methodology I call standpoint phenomenology and show how this methodology emphasizes experiences that are not “universal” but specific to certain social groups in order to uncover shared ontological structures of experience. Fanon's work illustrates two key theses of standpoint phenomenology: (1) the thesis of situated phenomenology and (2) the thesis of inverted phenomenological privilege. I also draw a deep connection between classical and standpoint phenomenology by showing that it is the phenomenological analysis of breakdown experiences (e.g., corporeal malediction) that enables a standpoint approach to phenomenology. This breakdown methodology is explicitly developed by Heidegger and utilized implicitly by Merleau‐Ponty. If I am right, standpoint phenomenology is both a natural development of and a considerable advance on the traditional methodology. This article, then, provides a better understanding of Fanon's place in the phenomenological tradition and, more broadly, makes explicit a new methodology for advancing phenomenological research.
Neonatal sepsis in alloimmune hemolytic disease of the fetus and newborn: A retrospective cohort study of 260 neonates
Authors: Jansen Sophie J. , Ree Isabelle M. C. , Broer Lana , de Winter Derek , de Haas Masja , Bekker Vincent , Lopriore Enrico 
Published: November 2022

AbstractBackgroundAmong neonates with hemolytic disease of the fetus and newborn (HDFN), we aimed to describe the frequency of central‐line use, indications for insertion, and incidence of confirmed and suspected sepsis, including antibiotic treatment over a 10‐year surveillance period.Study Design and MethodsAll neonates with HDFN admitted to our neonatal intensive care unit between January 2012 and December 2021 were included in this retrospective, cohort study. Annual proportions of infants with a central‐line and central‐line‐associated bloodstream infection (CLABSI) rates (per 1000 central‐line days and per 100 infants) were evaluated. Numbers of confirmed and suspected early‐ and late‐onset sepsis episodes were assessed over the entire study period.ResultsOf the 260 included infants, 25 (9.6%) were evaluated for suspected sepsis, with 16 (6.2%) having ≥1 confirmed sepsis episode. A total of 123 central‐lines were placed in 98 (37.7%) neonates, with impending exchange transfusion (ET) being the most frequent indication. Of the 34 (34.7%) neonates in whom a central‐line was placed due to impending ET, 11 (32.4%) received no ET. Overall CLABSI incidence was 13.58 per 1000 central‐line days. Neonates with a central‐line had a higher risk for confirmed late‐onset infection (RR 1.11, 95% CI: 1.04–1.20) and sepsis work‐up (RR 1.10, 95% CI: 1.03–1.17) compared to infants without a central‐line.ConclusionsSepsis incidence among neonates with HDFN remains high, in particular in those with a central‐line. Considering the substantial proportion of neonates with a central‐line without eventual ET, central‐line placement should be delayed until the likelihood of ET is high.
The last complex WIMPs standing
Authors: Bottaro Salvatore , Buttazzo Dario , Costa Marco , Franceschini Roberto , Panci Paolo , Redigolo Diego , Vittorio Ludovico 
Published: November 2022

AbstractWe continue the study of weakly interacting massive particles (WIMP) started in Bottaro et al. (Eur Phys J C 82:31, 2022), focusing on a single complex electroweak n-plet with non-zero hypercharge added to the Standard Model. The minimal splitting between the Dark Matter and its electroweak neutral partner required to circumvent direct detection constraints allows only multiplets with hypercharge smaller or equal to 1. We compute for the first time all the calculable WIMP masses up to the largest multiplet allowed by perturbative unitarity. For the minimal allowed splitting, most of these multiplets can be fully probed at future large-exposure direct detection experiments, with the notable exception of the doublet with hypercharge 1/2. We show how a future muon collider can fully explore the parameter space of the complex doublet combining missing mass, displaced track and long-lived track searches. In the same spirit, we study how a future muon collider can probe the parameter space of complex WIMPs in regions where the direct detection cross section drops below the neutrino floor. Finally, we comment on how precision observables can provide additional constraints on complex WIMPs.
Association of a Multiancestry Genome-Wide Blood Pressure Polygenic Risk Score With Adverse Cardiovascular Events
Authors: Parcha Vibhu , Pampana Akhil , Shetty Naman S. , Irvin Marguerite R. , Natarajan Pradeep , Lin Henry J. , Guo Xiuqing , Rich Stephen S. , Rotter Jerome I. , Li Peng , Oparil Suzanne , Arora Garima , Arora Pankaj 
Published: November 2022

Background:
Traditional cardiovascular risk factors and the underlying genetic risk of elevated blood pressure (BP) determine an individual’s composite risk of developing adverse cardiovascular events. We sought to evaluate the relative contributions of the traditional cardiovascular risk factors to the development of adverse cardiovascular events in the context of varying BP genetic risk profiles.

Methods:
Genome-wide polygenic risk score (PRS) was computed using multiancestry genome-wide association estimates among US adults who underwent whole-genome sequencing in the Trans-Omics for Precision program. Individuals were stratified into high, intermediate, and low genetic risk groups (>80th, 20–80th, and <20th centiles of systolic BP [SBP] PRS). Based on the ACC/AHA Pooled Cohort Equations, participants were stratified into low and high (10 year-atherosclerotic cardiovascular disease [CVD] risk: <10% or ≥10%) cardiovascular risk factor profile groups. The primary study outcome was incident cardiovascular event (composite of incident heart failure, incident stroke, and incident coronary heart disease).

Results:
Among 21 897 US adults (median age: 56 years; 56.0% women; 35.8% non-White race/ethnicity), 1 SD increase in the SBP PRS, computed using 1.08 million variants, was associated with SBP (β: 4.39 [95% CI, 4.13–4.65]) and hypertension (odds ratio, 1.50 [95% CI, 1.46–1.55]), respectively. This association was robustly seen across racial/ethnic groups. Each SD increase in SBP PRS was associated with a higher risk of the incident CVD (multivariable-adjusted hazards ratio, 1.07 [95% CI, 1.04–1.10]) after controlling for ACC/AHA Pooled Cohort Equations risk scores. Among individuals with a high SBP PRS, low atherosclerotic CVD risk was associated with a 58% lower hazard for incident CVD (multivariable-adjusted hazards ratio, 0.42 [95% CI, 0.36–0.50]) compared to those with high atherosclerotic CVD risk. A similar pattern was noted in intermediate and low genetic risk groups.

Conclusions:
In a multiancestry cohort of >21 000 US adults, genome-wide SBP PRS was associated with BP traits and adverse cardiovascular events. Adequate control of modifiable cardiovascular risk factors may reduce the predisposition to adverse cardiovascular events among those with a high SBP PRS.