bluuebunny/crossref_metadata_2025_split huggingface dataset:
Could an Earth-mass planet have existed between Jupiter and Saturn?
Authors: Dvorak R. , Cuntz M. 
Published: August 2022

Abstract
Motivated by previous studies about asteroids situated between Jupiter and Saturn, where long-term stable orbits with small eccentricities and low inclinations have been identified, we extend this research by placing an Earth-mass planet in the region between Jupiter and Saturn. We pursued numerical integrations of the equations of motions for the outer solar system encompassing Jupiter, Saturn, Uranus, and Neptune, as well as the additional Earth-mass planet. This 6-body system has been integrated with the Lie method for up to 500 Myr. The default initial stable zone for the Earth-mass planet has been assumed between 7.04 au and 7.31 au based on the results from previous studies by our group. We explored each region separately for 360 Earth-mass planets (assuming different mean anomalies) and inspected the respective escape times (ETs). The overall picture shows that the most stable orbits occur between 7.12 au and 7.28 au. Furthermore, the mean of the ETs for all 360 mean anomalies between 7.24 au and 7.12 au gradually decreases from ET=11 Myr to 0.9 Myr, although smaller values are obtained for the median due to outliers. To check the role of stability of the Earth-mass planet depending on its initial inclination, we focused on three regions (i.e., 7.23 au, 7.24 au, and 7.25 au) in terms of their ETs assuming i = 1°, 3°, and 5°; however, no significant differences were found. Hence, the general possibility of an Earth-mass planet situated between Jupiter and Saturn in the Solar System’s distant past is highly notable, although it would have been a transient event.
Selfing species has greater genetic diversity and less structure than related outcrossing species due to seed dispersal and population history
Authors: Zhao Jian-Li , Shao Guo-Jun , Sork Victoria , Wang Ya-Li , Li Li , Chen Hong-Fan , Wang Wen-Jing , Huang Mei-Yuan , Li Qingjun 
Published: August 2022

Abstract
Mating system is a crucial factor shaping the amount of genetic diversity and the fine-scale spatial genetic structure (FSGS), with selfing species usually having less diversity and stronger FSGS than outcrossing species. Such general conclusions resulted from comparisons among distant species, often neglecting consideration of co-effects of the population history, microhabitat and seed dispersal in different habitats. This study used two species in the genus Roscoea (Zingiberaceae) with contrasting mating systems to explore the formation of FSGS. Using thousands of single nucleotide polymorphisms (SNPs) found in outcrossing Roscoea cautleoides (RC) and selfing R. schneideriana (RS) located in different habitats from same region, we compared genetic diversity, FSGS and historical population dynamics between outlier (non-neutral) SNPs and neural SNPs, integrating with field observation of seed dispersal distance. RC has lower genetic diversity and showed stronger FSGS than RS, which conflicted with the general expectations. Outlier SNPs exhibited significantly larger genetic diversity and genetic differentiations among individuals within population suggested adaptive divergence to different microhabitats. Both RC and RS experienced bottlenecks that are consistent with the Last Glacial Maximum (~21 kyrs) and the last second Glacial Maximum (~150 kyrs), respectively. Field investigation indicated seeds of RS were transported from stock plants by ant significantly further than seeds of RC. Our results indicate that seed dispersal, different historical population dynamics, and environmental heterogeneity can over-ride the initial impact of pollen dispersal.
Recombinase Polymerase Amplification Assay with Lateral Flow Strips for Rapid Diagnosis of Candidiasis due to Candida parapsilosis
Authors: Wang Kun , Fan Shihui , Li Yuanyuan , Huo Li , Wang Yan , Wang Xizhen , Wang Lei 
Published: August 2022

Abstract
Background
Candida parapsilosis is a common cause of candidiasis among hospitalized patients, often surpassing Candida albicans. Due to the recent increase in C. parapsilosis infections, there is an urgent need for rapid, sensitive, and real-time on-site detection of nucleic acids for timely diagnosis of candidiasis.
Methods
We developed an assay for detection of C. parapsilosis by combining recombinase polymerase amplification (RPA) with a lateral flow strip (LFS). The RPA-LFS assay was used to amplify the beta-1,3-glucan synthase catalytic subunit 2 (FSK2) gene of C. parapsilosis with a primer-probe set optimized by introducing base mismatches (4 bases modified by the probe and one by the reverse primer) to achieve specific and sensitive detection of clinical samples.
Results
The RPA assays can rapidly amplify and visualize a target gene within 30 min, while the entire process can be completed within 40 min by pre-processing the sample.The sensitivity and specificity of the RPA-LFS assay were determined by analysis of 35 common clinical pathogens and 281 clinical samples against quantitative PCR.
Conclusions
The results confirmed that the proposed RPA-LFS assay is a reliable molecular diagnostic method for the detection of C. parapsilosis to meet the urgent need for rapid, specific, sensitive, and portable field testing.
Plasma host protein signatures correlating with Mycobacterium tuberculosis activity prior to and during antituberculosis treatment
Authors: NDIAYE Mame Diarra Bousso , RANAIVOMANANA Paulo , RASOLOHARIMANANA Tsikiniaina , RASOLOFO Voahangy , RATOVOSON Rila , HERINDRAINY Perlinot , RAKOTONIRINA Julio , SCHOENHALS Matthieu , HOFFMANN Jonathan , RAKOTOSAMIMANANA Niaina 
Published: August 2022

Abstract
Background
There is a need for rapid non-sputum-based tests to identify and treat patients infected with Mycobacterium tuberculosis (Mtb). In this study, performance of a human plasma protein signature for both TB triage and treatment monitoring has been evaluated.
Methods
A panel of seven host proteins CLEC3B, SELL, IGFBP3, IP10, CD14, ECM1 and C1Q were measured in the plasma of a prospective patient cohort undergoing anti-tubercular therapy to distinguish confirmed TB patients from control, to define culture slow and fast converters during an antituberculosis treatment and to monitor the treatment. To validate the protein signatures a Luminex xMAP® assay was used to quantify the proteins in unstimulated plasma isolated from the blood collected from HIV-negative pulmonary TB patients (ATB), at baseline and following 6-months of antituberculosis treatment, latently TB-infected individuals (LTBI) and healthy donors (HD). Protein signatures performances were evaluated using CombiROC algorithm and multivariate models.
Findings:
We measured the proteins in the plasma of 84 participants including 37 ATB, 24 LTBI and 23 HD. The seven plasma host proteins studied showed different levels between the TB clinical groups and when used separately or in combination, have variable performances for ATB triage and for treatment monitoring. Six of the plasma proteins (CLEC3B, SELL, IGFBP3, IP10, CD14 and C1Q) showed significant differences in normalised median fluorescence intensities when comparing ATB vs HD or LTBI while ECM1 revealed a significant association with early sputum culture conversion after 2 months following treatment (OR 0.004, p = 0.018). For both triage and treatment monitoring, a signature combining 4 host proteins markers (CLEC3B-ECM1-IP10-SELL) was identified, allowing to distinguish ATB from HD or LTBI (respectively, sensitivity = 94%, specificity = 92% and sensitivity = 89%, specificity = 91%) as well as the baseline distinction between slow vs fast sputum culture converters after two months of treatment (AUC = 0.87, sensitivity = 83%, specificity = 84%).
Conclusion
Our data demonstrate that using a combination of host plasma markers can generate a relevant biosignature for both TB triage and treatment monitoring meeting the WHO Target Product Profile for both purposes.
Design and validation of a multi-task, multi-phase protocol for real-world gait simulation
Authors: Scott Kirsty , Bonci Tecla , Francesca Salis , Alcock Lisa , Buckley Ellen , Gazit Eran , Hansen Clint , Schwickert Lars , Aminian Kamiar , Bertuletti Stefano , Caruso Marco , Chiari Lorenzo , Sharrack Basil , Maetzler Walter , Becker Clemens , Haussdorff Jeffrey M. , Vogiatzis Ioannis , Brown Philip , Din Silvia Del , Eskofier Björn , Paraschiv-Ionescu Anisoara , Keogh Alison , Cameron Kirk , Kluge Felix , Micó-Amigo M. Encarna , Mueller Arne , Neatrur Isabel , Niessen Martijn , Palmerini Luca , Sillen Henrik , Singleton David , Ullrich Martin , Vereijken Beatrix , Froelich Marcel , Brittan Gavin , Caulfield Brian , Koch Sarah , Carsin Anne-Elie , Garcia-Aymerich Judith , Kuederle Arne , Yarnall Alison , Cereatti Andrea , Mazzà Claudia 
Published: August 2022

Abstract
Background: Measuring mobility in daily life entails dealing with confounding factors arising from multiple sources, including pathological characteristics, patient specific walking strategies, environment/context, and purpose of the task. The primary aim of this study is to propose and validate a multi-task and multi-phase protocol for simulating real-world gait accounting for all these factors within a single set of observations carried out within a limited laboratory space, while ensuring minimisation of participant burden and safety.Methods: The protocol included eight motor tasks at varying speed, incline/steps, surface, path shape, cognitive demand, and included postures that may abruptly alter the participants’ strategy of walking. It was deployed in a convenience sample of 108 participants recruited from six cohort groups that included older healthy adults (HA) and participants with potentially altered mobility due to Parkinson’s Disease (PD), Multiple Sclerosis (MS), Proximal Femoral Fracture (PFF), Chronic Obstructive Pulmonary Disease (COPD) or Congestive Heart Failure (CHF). A novelty introduced in the protocol was the tiered approach to increase difficulty both within the same task (e.g., by allowing use of aids or armrests) and across tasks.Results: The protocol proved to be safe and feasible and the addition of the more complex tasks allowed a much greater spread in walking speeds to be achieved compared to standard straight walking trials. Furthermore, it allowed a relatively realistic representation of daily life relevant mobility aspects and can therefore be used for the validation of monitoring devices used in real life.Conclusions: The suitability of the protocol for measuring gait in a variety of pathological conditions suggests that it can also be used to detect changes in gait due to, for example, the onset or progression of a disease, or due to therapy.Trial registration: ISRCTN – 12246987.
Successful treatment of Balamuthia mandrillaris granulomatous amoebic encephalitis with the novel agent nitroxoline
Authors: Spottiswoode Natasha , Pet Douglas , Kim Annie , Gruenberg Katherine , Shah Maulik , Ramachandran Amrutha , Laurie Matthew , Zia Maham , Fouassier Camille , Boutros Christine , Lu Rufei , Zhang Yueyang , Servellita Venice , Bollen Andrew , Chiu Charles , Wilson Michael , Valdivia Liza , DeRisi Joseph 
Published: August 2022

Abstract
This case report describes a survivor of Balamuthia mandrillaris granulomatous amoebic encephalitis (GAE) after treatment with the novel agent nitroxoline, a drug typically used to treat urinary tract infections. Nitroxoline was previously identified in a screen of US and European approved drugs for amoebicidal activity against this pathogen. We describe its successful first use in treating this rare and deadly disease.
miR-9 and miR-218 modulate α7 nicotinic acetylcholine receptors levels via targeting of RIC-3 chaperone
Authors: Roshani Shima , Azizikhoshsirat Hana , Asadi Milad , Sadigh-Eteghad Saeed , Shanehbandi Dariush 
Published: August 2022

Abstract
Nicotinic acetylcholine receptors (nAChRs) have a role in a variety of pathophysiological conditions. Resistant to inhibitor cholinesterase (RIC-3) is a chaperone protein responsible for proper folding and assembly of α7 subunit of nAChRs. A variety of microRNAs, including miR-9 and miR-218, affect RIC-3. Thus, in the current study, the targeting of the RIC-3 gene with miR-9 and miR-218 was investigated. HEK-293 cells were transfected with miR-9 and miR-218 pre-mature miRNAs and were subjected to qRT-PCR, luciferase assay, and western blotting. The obtained results revealed that exogenous upregulation of miR-9/miR-218 further reduced RIC-3 mRNA and protein expression. In addition, luciferase assay illustrated that miR-9/miR-218 directly binds to the specific regions in RIC-3 3’-UTR and suppresses its expression. Also, it was found that miR-9/miR-218 mediated suppression of RIC-3 led to significant decreases of α7nAChR at protein levels. In conclusion, our findings implied that miR-9 and miR-218 might be involved in the receptor related-cellular signaling through modulating the RIC-3/α7 nAChR axis.
Hydrolysis of Regenerated Cellulose from Ionic Liquids and Deep Eutectic Solvent over Sulfonated Carbon Catalysts
Authors: Kim Han Ung , Kim Jong Wha , Seo Sumin , Park Young-Kwon , Jae Jungho 
Published: August 2022

Abstract
The efficient hydrolysis of cellulose into its monomer unit such as glucose or valuable cello-oligosaccharides is the critical step for the cost-effective production of biofuels and biochemicals. However, the current cellulose hydrolysis process involves high energy-demanding pretreatment (e.g., ball-milling) and long reaction times (>24h). Herein, we investigated the feasibility of the dissolution/regeneration (DR) of cellulose in ionic liquids (ILs) and deep eutectic solvent (DES) as an alternative to ball-milling pretreatment for the effective hydrolysis of cellulose. Because chlorine-based solvents are reported to be most active for biomass pretreatment, [EMIM]Cl and [DMIM]DMP were selected as the IL molecules, and choline chloride-lactic acid was selected as the DES molecule. The level of the crystallinity reduction of the regenerated cellulose were analyzed by the XRD and SEM measurements. The hydrolysis kinetics of the regenerated cellulose from ILs and DES were examined at 150 °C using sulfonated carbon catalysts and compared with the ball-milled cellulose. Overall, the cellulose pretreatment using the ILs and the DES had similar or superior kinetics for cellulose hydrolysis to the conventional ball milling treatment, suggesting a possibility to replace the current high energy-demanding ball-milling process with the energy-saving DR process. In addition, the utilization of supercritical carbon dioxide-induced carbonic acid as an in-situ acid catalyst for the enhanced hydrolysis of cellulose was presented for the first time.
Description of covalent bond in terms of generalized charges. Potential and dissociation energy of homonuclear compound
Authors: Dolgonosov Anatoly M. 
Published: August 2022

Abstract
The theory of generalized charges is an asymptotic approximation of quantum mechanics for interatomic forces. It is based on the model of multicomponent electron gas, which extends the Thomas-Fermi model of inhomogeneous electron gas to pair electronic states. The present research takes in consideration the participation of the field of generalized charges in interatomic bonds. Herein, the definition of generalized charges (GC) by means of the overlap integral of the wave functions of valence electrons is given, and properties of GC are found out. Equations are derived for the potential and length of a homopolar bond, and dependence of these parameters on the generalized charges is shown. The dissociation energy of a diatomic molecule is expressed in terms of the nuclear charges, the function and multiplicity of the covalent bond, the angular momentum, and the vibrational energy of the binding electrons. The parametrization rules are substantiated and an a priori calculation of the dissociation energy of diatomic homonuclear molecules is carried out against the electronic configuration of the bond electrons and the nuclear charge. The calculation results for homonuclear compounds of the elements of the first four rows of the Periodic table are shown to be of satisfactory accuracy.
Preparation and Characterization of Poly (Vinyl Alcohol)/ PANI@CuS Hybrid Nanocomposites for Optoelectronic Application
Authors: Sangamesha M. A. , R Kavya , Madhukar B. S. 
Published: August 2022

Abstract
Inclusion of conducting polymers coated inorganic nano particles in to semiconducting host polymers opens a new window to modify physical and chemical properties of the semiconducting polymers. An in-suite polymerization method was employed for the synthesis of polyaniline (PANI) composites of with copper sulphide (PANI@CuS) and later introduced to polyvinyl alcohol (PVA). In this present work PVA/PANI@CuS hybrid nanocomposites (NCs) were prepared by eco-friendly solution casting method with varying concentration of PANI@CuS viz. 0, 1, 2, 3 and 4 wt%. the prepared polymer hybrid nanocomposites were subjected to various analytical technique to evaluate their morphological, electrical, optical and surface properties. The X-ray diffraction and FT-IR studies display the molecular interaction between CuS, PANI and PVA. UV- Visible shows reduced band gap from 6.17 to 3.43 whereas refractive index increases from 1.40–1.73 which gives the information about the optical properties furthermore, photoluminescence spectra show a stoke shift of ~ 150 nm towards higher wavelenth which opens new window for the photovoltaic applications. Scanning electron microscope (SEM) is used to study the morphology, electrical properties were analysed by DC electrical conductivity and found that the conduction mechanism follows Poole-Frenkel effect. The obtained results show that addition of PANI@CuS greatly enhances the opto-electronic properties of the host polymer matrix.
Influence of cooking methods on in vitro bioaccessibility of phenolics, flavonoids, and antioxidant activity of red cabbage
Authors: Ávila Suelen , Zalamanski Sara , Tanikawa Lilian Mitsuko , Kruger Claudia Carneiro Hecke , Ferreira Sila Mary Rodrigues 
Published: August 2022

Abstract
Red cabbage is rich in phytochemical compounds, and its consumption, either raw or cooked, has been linked to the prevention of several diseases. This work aimed to investigate the influence of cooking methods on in vitro bioaccessibility of phenolics and antioxidant activity of red cabbage. The vegetable was subjected to boiling, steaming, and microwaving for different times to evaluate color parameters, total phenolic (TPC), total flavonoid (TFC), anthocyanin content (AC), and antioxidant activity (FRAP, DPPH, and ABTS). The phytochemical bioaccessibility before and after cooking was also evaluated by in vitro simulated digestion. Steaming showed the most significant retention of the compounds after 20 and 25 minutes of cooking (72-86% for TPC, 72-77% for TFC, 75-79% for FRAP, 84-91% for DPPH, 70-83% for ABTS), followed by microwaving, which was more stable in 10 minutes. Microwaving decreased TFC and AC over time. Boiling did not show significant differences between the cooking times and showed more than 50% of losses of TPC, TFC, and AC and 30 to 60% of antioxidant activity. Steaming was the best cooking method, showing the most significant tendency to black coloration (< L*). In 10 minutes, it still showed the highest percentages of increase in TPC and the minor losses of TFC and AC in the gastric and intestinal phases. Steaming also increased the antioxidant after digestion when compared to uncooked red cabbage. These results are important to help consumers choose the most effective cooking method for red cabbage to retain its health-promoting components.
Application of split pancreatic drainage tube in laparoscopic pancreaticoduodenectomy
Authors: Tu Jianhua , Huang Changwen , Xu Wenyan , Gong Shuaichang , Cao Zhenjun , Wan Ping , Ying Junxiang , Rao Xuefeng 
Published: August 2022

Abstract
Background
Laparoscopic pancreaticoduodenectomy (LPD) is a classic surgical method for diseases, such as tumors at the lower end of the common bile duct, pancreatic head, and benign and malignant tumors of the duodenum. Postoperative pancreatic fistula (POPF) is one of the most serious complications of LPD. In this study, we introduced a separate stent drainage method to reduce the occurrence of grade B or C POPF and its related complications.
Methods
To reduce the incidence of grade B or C POPF and other complications after LPD, we applied a split pancreatic drainage tube combined with the characteristics of internal and external stent drainage. Between January 2018 and May 2022, 12 patients underwent placement of the split pancreatic drainage tube during LPD.Data on operation time, duration of pancreaticojejunostomy (PJ), intraoperative blood loss, first postoperative exhaust time, postoperative hospital stay, incidence of POPF and other complications were collected and analyzed.
Results
The average operation time was 280 ± 42 min, average time for PJ was 35 ± 6 min, and average estimated blood loss was 200 ± 40 mL. Biochemical leakage occurred in two patients (16.7%), whereas no grade B or C POPF, other related complications, and deaths occurred within 30 days after the operation.
Conclusion
Split pancreatic drainage tube effectively converts external stent drainage into internal stent drainage. It fully combines internal and external drainage characteristics, effectively reduces the incidence of postoperative POPF and other related complications of grade B or C, and provides a new choice for clinicians to make decisions.
Individualizing mechanical ventilation: Titration of driving pressure to pulmonary elastance through Young’s modulus in an acute respiratory distress syndrome animal model
Authors: Mingote Álvaro , García Ramsés Marrero , González Martín Santos , Díaz Raquel Castejón , Antón Clara Salas , Nuñez Juan Antonio Vargas , García-Fernández Javier 
Published: August 2022

Abstract
Background: Mechanical ventilation increases the risk of lung damage (VILI). Some authors propose that the way to reduce VILI is to find the threshold of driving pressure below which VILI is minimized. In this study, we propose a method to titrate the driving pressure to pulmonary elastance in an acute respiratory distress syndrome model using Young’s modulus and its consequences on ventilatory-induced lung injury. Material and methods: 20 Wister Han male rats were used. After generating an acute respiratory distress syndrome, two groups were studied: a) standard protective mechanical ventilation: 10 rats received 150 minutes of mechanical ventilation with driving pressure = 14 cm H2O, tidal volume < 6mL/kg) and b) individualized mechanical ventilation: 10 rats received 150 minutes of mechanical ventilation with an individualized driving pressure according to their Young’s modulus. In both groups, an individualized PEEP was programmed in the same manner. We analyzed the concentration of IL-6, TNF – α, and IL -1ß in BAL and the acute lung injury score in lung tissue postmortem. Results: Global driving pressure was different between the groups (14 vs 11 cm H2O, p = 0.03). The individualized mechanical ventilation group had lower concentrations in bronchoalveolar lavage of IL-6 (270 vs 155 pg/mL, p = 0.02), TNF – α (292 vs 139 pg/mL, p < 0.01) and IL – 1ß (563 vs 131 pg/mL, p = 0.05). They presented lower proportion of lymphocytes (96% vs 79%, p = 0.05) as well as lower lung injury score (6.0 vs 2.0, p = 0.02). Conclusion: In our model, individualization of DP to pulmonary elastance through Young’s modulus decreases lung inflammation and structural lung injury without a significant impact on oxygenation.