bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expert consensus on pulpotomy in the management of mature permanent teeth with pulpitis
Authors: Zhang Lu , Lin Chen , Chen Zhuo , Yue Lin , Yu Qing , Hou Benxiang , Ling Junqi , Liang Jingping , Wei Xi , Chen Wenxia , Qiu Lihong , Li Jiyao , Niu Yumei , Lin Zhengmei , Cheng Lei , He Wenxi , Wang Xiaoyan , Huang Dingming , Huang Zhengwei , Niu Weidong , Zhang Qi , Zhang Chen , Yang Deqin , Yu Jinhua , Zhao Jin , Pan Yihuai , Ma Jingzhi , Deng Shuli , Xie Xiaoli , Meng Xiuping , Yang Jian , Zhou Xuedong , Chen Zhi 
Published: January 2025

AbstractPulpotomy, which belongs to vital pulp therapy, has become a strategy for managing pulpitis in recent decades. This minimally invasive treatment reflects the recognition of preserving healthy dental pulp and optimizing long-term patient-centered outcomes. Pulpotomy is categorized into partial pulpotomy (PP), the removal of a partial segment of the coronal pulp tissue, and full pulpotomy (FP), the removal of whole coronal pulp, which is followed by applying the biomaterials onto the remaining pulp tissue and ultimately restoring the tooth. Procedural decisions for the amount of pulp tissue removal or retention depend on the diagnostic of pulp vitality, the overall treatment plan, the patient’s general health status, and pulp inflammation reassessment during operation. This statement represents the consensus of an expert committee convened by the Society of Cariology and Endodontics, Chinese Stomatological Association. It addresses the current evidence to support the application of pulpotomy as a potential alternative to root canal treatment (RCT) on mature permanent teeth with pulpitis from a biological basis, the development of capping biomaterial, and the diagnostic considerations to evidence-based medicine. This expert statement intends to provide a clinical protocol of pulpotomy, which facilitates practitioners in choosing the optimal procedure and increasing their confidence in this rapidly evolving field.
Emerging regulatory mechanisms and functions of biomolecular condensates: implications for therapeutic targets
Authors: Jeon Soyoung , Jeon Yeram , Lim Ji-Youn , Kim Yujeong , Cha Boksik , Kim Wantae 
Published: January 2025

AbstractCells orchestrate their processes through complex interactions, precisely organizing biomolecules in space and time. Recent discoveries have highlighted the crucial role of biomolecular condensates—membrane-less assemblies formed through the condensation of proteins, nucleic acids, and other molecules—in driving efficient and dynamic cellular processes. These condensates are integral to various physiological functions, such as gene expression and intracellular signal transduction, enabling rapid and finely tuned cellular responses. Their ability to regulate cellular signaling pathways is particularly significant, as it requires a careful balance between flexibility and precision. Disruption of this balance can lead to pathological conditions, including neurodegenerative diseases, cancer, and viral infections. Consequently, biomolecular condensates have emerged as promising therapeutic targets, with the potential to offer novel approaches to disease treatment. In this review, we present the recent insights into the regulatory mechanisms by which biomolecular condensates influence intracellular signaling pathways, their roles in health and disease, and potential strategies for modulating condensate dynamics as a therapeutic approach. Understanding these emerging principles may provide valuable directions for developing effective treatments targeting the aberrant behavior of biomolecular condensates in various diseases.
Combined nerve and tendon transfer strategy for the restoration of grasp in tetraplegia; a case report
Authors: Bunketorp Käll Lina , Wangdell Johanna , Reinholdt Carina , Fridén Jan 
Published: January 2025

Abstract
Introduction
By combining nerve and tendon transfer procedures, a more versatile hand function can be expected. Here we report the long-term outcomes of novel, individualized reconstruction strategies using combined nerve and tendon transfer procedures (CNaTT) to restore prehension and grasp in two patients with tetraplegia.

Case presentation
Two women, 45 years of age, underwent bilateral nerve transfer according to the Bertelli S-PIN (supinator to posterior interosseous nerve transfer) procedure. The grip reconstruction included tendon transfers using brachioradialis to flexor pollicis longus and extensor carpi radialis longus to flexor digitorum profundus, as well as balancing tenodesis, arthrodesis procedures and intrinsic reconstruction. At 6 months, the patients’ pinch and grasp strength ranged between 1.0–2.0 and 2.2–5.0 kg, respectively, concomitant with improvements in activity and occupational performance. At 4–7 years after the grip reconstruction, both patients had full metacarpophalangeal (MCP) extension scoring M5 and M4, as well as full thumb extension scoring M5 and M4 on the right side. On the left side, MCP extension was weaker for both patients (M1/M2), whereas the thumb could extend against gravity (M3/M4). The maximal 1st webspace opening measured between 5 and 11 cm. Pinch strength measured between 1.25 and 2.6 kg, and whole hand grip strength between 3.9 and 7.8 kg. The patients’ grasps could fit around 80 and 50 mm wide cylinders using a normal right-handed grasp.

Discussion
The CNaTT procedure successfully restored useful grasp and release function with long-lasting effects. A large-scale controlled study is needed to confirm these findings.
Clinical validity of fluorescence-based devices versus visual-tactile method in detection of secondary caries around resin composite restorations: diagnostic accuracy study
Authors: Adly Aya Mohamed , Ibrahim Shereen Hafez , El-Zoghbi Amira Farid 
Published: January 2025

Abstract
Objectives
To assess the validity of light-induced and laser-induced fluorescence devices compared to the visual-tactile method for detecting secondary caries around resin composite restorations.

Materials and Methods
The study included 20 participants with 30 resin-composite restored teeth. Restorations’ margins were examined using three diagnostic methods: the visual-tactile method (FDI criteria), the light-induced fluorescence camera (VistaCam iX), and the laser-induced fluorescence device (DIAGNOdent pen), and the reference was visual inspection after removal of defective restorations. The validity of each method was evaluated. Inter-examiner reliability was calculated using Cohen’s kappa statistics. The level of significance was set at P = 0.05.

Results
DIAGNOdent pen showed the highest sensitivity (100%) followed by VistaCam (98.82%) and the visual-tactile method (98.82%) at the enamel threshold. DIAGNOdent pen and VistaCam had lower specificity values than the visual-tactile method (81.69%, 76.06%, and 88.73% respectively). At the dentin threshold, DIAGNOdent pen yielded the highest sensitivity (89.36%), whereas VistaCam had the lowest (8.51%). The sensitivity of the visual-tactile method was low (57.45%) whereas all diagnostic methods had high specificity. There was perfect agreement in inter-examiner reliability for all assessment methods (Kappa 0.858–0.992).

Conclusions
Both fluorescence-based devices and the visual-tactile method are reliable for detecting secondary caries around resin composite restorations. DIAGNOdent pen is accurate in enamel and dentin, while VistaCam and the visual-tactile method can detect secondary caries in enamel only.

Clinical Relevance
Fluorescence-based devices could be used as a valuable aid to supplement or as a second opinion after the visual-tactile method.

Trial registration
The study was listed on www.clinicaltrials.gov with registration number (NCT04426604) on 11/06/2020.
Alternative splicing in the DBD linker region of p63 modulates binding to DNA and iASPP in vitro
Authors: Lotz Rebecca , Osterburg Christian , Chaikuad Apirat , Weber Sabrina , Akutsu Masato , Machel Anne Christin , Beyer Ulrike , Gebel Jakob , Löhr Frank , Knapp Stefan , Dobbelstein Matthias , Lu Xin , Dötsch Volker 
Published: January 2025

AbstractThe transcription factor p63 is expressed in many different isoforms as a result of differential promoter use and splicing. Some of these isoforms have very specific physiological functions in the development and maintenance of epithelial tissues and surveillance of genetic integrity in oocytes. The ASPP family of proteins is involved in modulating the transcriptional activity of the p53 protein family members, including p63. In particular, iASPP plays an important role in the development and differentiation of epithelial tissues. Here we characterize the interaction of iASPP with p63 and show that it binds to the linker region between the DNA binding domain and the oligomerization domain. We further demonstrate that this binding site is removed in a splice variant of p63 where a stretch of five amino acids is replaced with a single alanine residue. This stretch contains a degenerate class II SH3 domain binding motif that is responsible for interaction with iASPP, as well as two positively charged amino acids. Moreover, the concomitant loss of the charged amino acids in the alternatively spliced version decreases the affinity of p63 to its cognate DNA element two- to threefold. mRNAs encoding full-length p63, as well as its alternatively spliced version, are present in all tissues that we investigated, albeit in differing ratios. We speculate that, through the formation of hetero-complexes of both isoforms, the affinity to DNA, as well as the interaction with iASPP, can be fine-tuned in a tissue-specific manner.
Collective dynamical regimes predict invasion success and impacts in microbial communities
Authors: Hu Jiliang , Barbier Matthieu , Bunin Guy , Gore Jeff 
Published: January 2025

AbstractThe outcomes of ecological invasions may depend on either characteristics of the invading species or attributes of the resident community. Here we use a combination of experiments and theory to show that the interplay between dynamics, interaction strength and diversity determine the invasion outcome in microbial communities. We find that the communities with fluctuating species abundances are more invasible and diverse than stable communities, leading to a positive diversity–invasibility relationship among communities assembled in the same environment. As predicted by theory, increasing interspecies interaction strength and species pool size leads to a decrease of invasion probability in our experiment. Our results show a positive correspondence between invasibility and survival fraction of resident species across all conditions. Communities composed of strongly interacting species can exhibit an emergent priority effect in which invader species are less likely to colonize than species in the original pool. However, if an invasion is successful, its ecological effects on the resident community are greater when interspecies interactions are strong. Our findings provide a unified perspective on the diversity–invasibility debate by showing that invasibility and invasion effect are emergent properties of interacting species, which can be predicted by simple community-level features.
Mycosynthesis of chitosan-selenium nanocomposite and its activity as an insecticide against the cotton leafworm Spodoptera littoralis
Authors: Ibrahiem Sohila A. , Reda Fifi M. , Abd-ElAzeem Eman M. , Hashem Mostafa S. , Ammar Hala A. 
Published: January 2025

AbstractThe cotton leafworm, Spodoptra littoralis, causes great damage to cotton crops. A new, safer method than insecticide is necessary for its control. Selenium nanoparticles (SeNPs) are metalloid nanomaterial, with extensive biological activities. They have low toxicity and can be used safely in plant disease management. In this study, we successfully bio-fabricated selenium nanoparticles and chitosan-selenium nanocomposite (Ch-SeNPs) using a fungal cell-free filtrate of Penicillium griseofulvum. The biosynthesized nanomaterials were initially detected optically by the formation of a red color in the solution mixture and the appearance of a strong plasmon resonance peak at 240–300 nm. The biosynthesized nanomaterials were fully characterized by UV–visible spectroscopy, transmission electron microscopy, dynamic light scattering, energy dispersive X-ray, inductively coupled plasma spectroscopy, and Fourier transform infrared. We tested the anti-insect activities of SeNPs, and Ch-SeNPs against larvae of S. littoralis compared to spore suspensions of P. griseofulvum. The results indicated that Ch-SeNPs followed by SeNPs gave a significantly higher mortality percentage than the spore suspension of the tested fungus. The highest production of all biosynthesized nanomaterials was detected after 7 days at 40 °C under alkaline conditions (pH 9). The average size diameter of SeNPs and Ch-SeNPs were 91.25 and 67.41 nm with zeta potential − 8.05 and + 41 mV, respectively. Both Ch-SeNPs and SeNPs gave high mortality rates and low values of LC50 and LC90 for both larvae and pupae. Ch-SeNPs showed stronger activity against S. littoralis than SeNPs and spore suspension at all experimental conditions. Cytotoxicity experiments indicated their safety against honeybee populations. The current study reveals the significant ultrastructure impact of SeNPs on larvae. These findings suggest that selenium nanoparticles and nanocomposite can be fabricated with a costless easy route using fungal filtrate, and they can be used safely in pest control systems that are safe for honeybee populations. It is the first report about the application of Ch-SeNPs as an anti-insect agent.
Spatial transcriptomics unveils estrogen-modulated immune responses and structural alterations in the ectocervical mucosa of depot medroxyprogesterone acetate users
Authors: Kaldhusdal Vilde , Boger Mathias Franzen , Tjernlund Annelie , Burgener Adam D. , Bradley Frideborg , Lajoie Julie , Omollo Kenneth , Kimani Joshua , Fowke Keith , Czarnewski Paulo , Broliden Kristina 
Published: January 2025

AbstractThe injectable contraceptive, depot medroxyprogesterone acetate (DMPA), is associated with compromised cervical mucosal barriers. High-resolution spatial transcriptomics is applied here to reveal the spatial localization of these altered molecular markers. Ectocervical tissue samples from Kenyan sex workers using DMPA, or non-hormonal contraceptives, underwent spatial transcriptomics and gene set enrichment analyses. Integrated systemic estradiol levels and bulk tissue gene expression data from a larger cohort enhanced the study’s scope. Unsupervised clustering unveiled four epithelial and seven submucosal layers, showcasing spatially restricted and diverse functional epithelial responses, and a less structured submucosal spatial ordering. DMPA associated with mucosal-wide immunoglobulin gene upregulation, verified by CD20+ B-cell immunostaining, and upregulated immune markers adjacent to the basal membrane. Downregulated genes represented spatially restricted disrupted epithelial barrier integrity and submucosal extracellular matrix dysfunction. The transcriptional profile was associated with markers of estrogen regulation. Collectively, our findings reveal estrogen-modulated distinct ectocervical transcriptional profiles associated with DMPA usage. While upregulation of immunoglobulin genes occurs throughout the mucosa, activation of innate immune responses and dysregulation of barrier integrity markers are spatially restricted. These results extend previous analyses using bulk transcriptomics and provide insights into the molecular landscape influenced by DMPA, shedding light on contraceptive effects and health implications.
Characterisation of precursory seismic activity towards early warning of landslides via semi-supervised learning
Authors: Murray David , Stankovic Lina , Stankovic Vladimir , Pytharouli Stella , White Adrian , Dashwood Ben , Chambers Jonathan 
Published: January 2025

AbstractThis study demonstrates that machine learning from seismograms, obtained from commonly deployed seismometers, can identify the early stages of slope failure in the field. Landslide hazards negatively impact the economy and public through disruption, damage of infrastructure and even loss of life. Triggering factors leading to landslides are broadly understood, typically associated with rainfall, geological conditions and steep topography. However, early warning at slope scale of an imminent landslide is more challenging. Through semi-supervised learning for seismic event detection from continuous seismic recordings over a period of about 10 years, we demonstrate that timely landslide induced displacement prediction is possible, providing the basis for landslide early warning systems. Our proposed methodology detects and characterises seismic precursors to landslide events making use of seismic recordings near an active slow moving earth slide-flow using a semi-supervised Siamese network. This data driven methodology identifies increase in microseismicity, and the change in the frequency spectrum of that microseismicity which identify key stages prior to a failure: ‘rest’, ‘precursor’ and ‘active’. Due to the semi-supervised nature of Siamese networks, the methodology is adaptable to discovering new types of distinct events, making it an ideal solution for precursor detection at new sites.
Research on design and control method of active vibration isolation system based on piezoelectric Stewart platform
Authors: Fang Zhiyi , Yu Zhiliang , Huang Qingping , Wang Yanfen , Gu Xingsheng 
Published: January 2025

AbstractSpace payloads in orbit are vulnerable to small vibrations from satellite platforms, which can degrade their performance. Traditional methods typically involve installing a passive vibration isolation system between the platform and the payload. However, such systems are usually effective only for high-frequency, large-amplitude vibrations and perform poorly in isolating low-frequency vibrations and resonances below 10 Hz. To address this limitation, this paper proposes an active vibration isolation system using a 6-degrees-of-freedom Stewart platform driven by piezoelectric actuators. First, the characteristics of the Stewart platform are analyzed and modeled, with the deformation displacement of each leg calculated through decoupling, allowing for high-precision servo control. Next, given the inherent hysteretic nonlinearity of piezoelectric ceramics, which significantly affects positioning accuracy, the hysteresis mechanism of the actuators is analyzed, and a phenomenological mathematical model based on Bouc–Wen operators is established. A Modified particle swarm optimization (MPSO) method is proposed for identifying the model’s nonlinear parameters, significantly enhancing the optimization efficiency. Finally, feedforward inverse compensation and feedback linearization methods are introduced. Experimental results verify that the designed active–passive vibration isolation system greatly improves both the positioning accuracy of the piezoelectric actuators and the active vibration isolation performance of the platform.
A computational and structural approach to identify malignant non-synonymous FOXM1 single nucleotide polymorphisms in triple-negative breast cancer
Authors: Chatterjee Prarthana , Banerjee Satarupa 
Published: January 2025

AbstractThe proliferation-specific oncogenic transcription factor, FOXM1 is overexpressed in primary and recurrent breast tumors across all breast cancer (BC) subtypes. Intriguingly, FOXM1 overexpression was found to be highest in Triple-negative breast cancer (TNBC), the most aggressive BC with the worst prognosis. However, FOXM1-mediated TNBC pathogenesis is not completely elucidated. Single nucleotide polymorphisms (SNPs) are the most common genetic variations causing functional and structural aberrations in proteins enhancing cancer susceptibility. This computational investigation attempted to identify the malignant FOXM1 non-synonymous SNPs (nsSNPs) and evaluate their role in affecting the conformational and functional stability, evolutionary conservation, post-translational modifications, and malignant susceptibility of the protein. Out of a huge data pool of 8826 FOXM1 SNPs using several in-silico sequence-based tools and structural approaches, four SNPs viz. E235Q, R256C, G429E and S756P were identified as pathogenic nsSNPs and among the shortlisted variants molecular dynamics simulations identified E235Q as the most damaging malignant SNP, followed by S756P. Additionally, the defective drug and DNA binding motif of E235Q and S756P were also determined in our study. Thus, although further in-vitro validations are awaited the findings of this in-silico work can be used as a blueprint for malignant nsSNP identification of FOXM1 aiding in clinical TNBC therapeutics.
An intelligent incentive-based demand response program for exhaustive environment constrained techno-economic analysis of microgrid system
Authors: Dey Bishwajit , Sharma Gulshan , Bokoro Pitshou N. , Dutta Soham 
Published: January 2025

AbstractThe cost-effective scheduling of distributed energy resources through sophisticated optimization algorithms is the main focus of recent work on microgrid energy management. In order to improve load factor and efficiency, load-shifting techniques are frequently used in conjunction with additional complex constraints such as PHEV scheduling and battery life assessment. Pollutant reduction, however, is rarely highlighted as a primary goal. An incentive-based demand response (IBDR) is introduced in the proposed work to close this gap and promote load curtailment during peak hours. IBDR policy rewards participant customers with incentives for load curtailment which in turn lowers emissions and generation costs. Furthermore, a trade-off approach ensures both environmental and economic sustainability by striking a balance between cost reduction and emission reduction. Considering the fact in view that the 30–40% of the microgrid customers are willing to participate in the IBDR program, six different scenarios that have been analysed, each of which involves various levels of grid participation and different approaches to pricing in the electricity market. These scenarios also include the implementation of demand response programmes. Differential evolution algorithm was used as the optimization tool for the study. The results achieved for all the scenarios demonstrate the suitability and effectiveness of implementing the suggested IBDR strategy in terms of cost savings. According to numerical results reported, the generating cost decreased by 10–13% with the inclusion of IBDR. Additionally, a 6–8% reduction in peak and 4–5% improvement in load factor was also realised as a positive impact of the IBDR policy. The weighted economic emission dispatch algorithm offered a balanced solution that considered both the minimum generation cost and emissions for various load models in the microgrid system.
Robust Cartesian space tracking control of robot manipulators driven by BLDC motors: An observer based partial state feedback approach
Authors: Unver Sukru , Saka Irem , Selim Erman , Tatlicioglu Enver , Zergeroglu Erkan , Alci Musa 
Published: January 2025

AbstractThis study addresses the end‐effector position tracking control of robot manipulators actuated by brushless direct current (BLDC) motors under the constraint that the entire electromechanical system (i.e. kinematic, dynamic, and electrical) contains uncertain parameters, and both joint velocity and Cartesian space velocity measurements are unavailable. Specifically, a partial state feedback controller (requiring phase current measurements of BLDC motors) is presented in conjunction with a novel robust velocity observer formulation that achieves semi‐global uniform ultimate boundedness of the Cartesian space position tracking error. The stability of the closed‐loop system is ensured via Lyapunov‐type arguments. To demonstrate the efficacy of the proposed design, an experimental study is conducted on an in‐house developed, two degree of freedom planar robot manipulator driven by BLDC motors.