bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful Adrenal Vein Sampling Using Dexamethasone Premedication in Patients With Iodine Contrast Media Allergy
Authors: Younes Nada , Therasse Eric , Bourdeau Isabelle , Lacroix André 
Published: June 2022

Abstract

Context
Preparation of patients with iodine contrast media (ICM) allergy who require adrenal vein sampling (AVS) to establish source of aldosterone excess of their confirmed primary aldosteronism (PA) is controversial. Usual premedication with high-dose prednisone can interfere with cortisol determinations, possibly altering the aldosterone to cortisol ratios for the identification of lateralized aldosterone excess.

Objective
We aimed to evaluate the efficacy and safety of premedication with high-dose dexamethasone to perform AVS in patients with ICM.

Methods
One hundred and seventy-seven consecutive patients with confirmed PA who underwent bilateral simultaneous basal and post-ACTH bolus AVS at our center between January 2010 and December 2020 were retrospectively analyzed for history of ICM allergy. A total of 7 patients (4%) with previous allergic reactions to ICM were prepared with 3 doses of 7.5 mg dexamethasone premedication rather than the usual 50 mg of prednisone.

Results
No breakthrough allergic reactions were reported in the 7 patients. Despite adequate serum cortisol suppression following dexamethasone, the basal and post-ACTH selectivity index were respectively &gt; 2 and &gt; 5 bilaterally in all patients, confirming adequate cannulation of both adrenal veins. Four patients had lateralized ratios (A/C ratio &gt; 2 basally and &gt; 4 post-ACTH), while 3 had bilateral source during AVS study. In the 3 patients undergoing unilateral adrenalectomy for lateralized source and contralateral suppression and adequate follow-up data, cure of PA was achieved at mean 58 months postoperatively.

Conclusion
AVS using dexamethasone premedication is safe and accurate for diagnosing the source of aldosterone excess in patients with PA and ICM allergy.
The regenerative capacity of neonatal tissues
Authors: Montero Angela M. , Huang Alice H. 
Published: June 2022

ABSTRACT
It is well established that humans and other mammals are minimally regenerative compared with organisms such as zebrafish, salamander or amphibians. In recent years, however, the identification of regenerative potential in neonatal mouse tissues that normally heal poorly in adults has transformed our understanding of regenerative capacity in mammals. In this Review, we survey the mammalian tissues for which regenerative or improved neonatal healing has been established, including the heart, cochlear hair cells, the brain and spinal cord, and dense connective tissues. We also highlight common and/or tissue-specific mechanisms of neonatal regeneration, which involve cells, signaling pathways, extracellular matrix, immune cells and other factors. The identification of such common features across neonatal tissues may direct therapeutic strategies that will be broadly applicable to multiple adult tissues.
Daw1 regulates the timely onset of cilia motility during development
Authors: Bearce Elizabeth A. , Irons Zoe H. , Craig Samuel B. , Kuhns Colin J. , Sabazali Cynthia , Farnsworth Dylan R. , Miller Adam C. , Grimes Daniel T. 
Published: June 2022

ABSTRACT
Motile cilia generate cell propulsion and extracellular fluid flows that are crucial for airway clearance, fertility and left-right patterning. Motility is powered by dynein arm complexes that are assembled in the cytoplasm then imported into the cilium. Studies in Chlamydomonas reinhardtii showed that ODA16 is a cofactor which promotes dynein arm import. Here, we demonstrate that the zebrafish homolog of ODA16, Daw1, facilitates the onset of robust cilia motility during development. Without Daw1, cilia showed markedly reduced motility during early development; however, motility subsequently increased to attain close to wild-type levels. Delayed motility onset led to differential effects on early and late cilia-dependent processes. Remarkably, abnormal body axis curves, which formed during the first day of development due to reduced cilia motility, self-corrected when motility later reached wild-type levels. Zebrafish larva therefore possess the ability to survey and correct body shape abnormalities. This work defines Daw1 as a factor which promotes the onset of timely cilia motility and can explain why human patients harboring DAW1 mutations exhibit significant laterality perturbations but mild airway and fertility complications.
Centriole distal-end proteins CP110 and Cep97 influence centriole cartwheel growth at the proximal end
Authors: Aydogan Mustafa G. , Hankins Laura E. , Steinacker Thomas L. , Mofatteh Mohammad , Saurya Saroj , Wainman Alan , Wong Siu-Shing , Lu Xin , Zhou Felix Y. , Raff Jordan W. 
Published: June 2022

ABSTRACT
Centrioles are composed of a central cartwheel tethered to nine-fold symmetric microtubule (MT) blades. The centriole cartwheel and MTs are thought to grow from opposite ends of these organelles, so it is unclear how they coordinate their assembly. We previously showed that in Drosophila embryos an oscillation of Polo-like kinase 4 (Plk4) helps to initiate and time the growth of the cartwheel at the proximal end. Here, in the same model, we show that CP110 and Cep97 form a complex close to the distal-end of the centriole MTs whose levels rise and fall as the new centriole MTs grow, in a manner that appears to be entrained by the core cyclin-dependent kinase (Cdk)–Cyclin oscillator that drives the nuclear divisions in these embryos. These CP110 and Cep97 dynamics, however, do not appear to time the period of centriole MT growth directly. Instead, we find that changing the levels of CP110 and Cep97 appears to alter the Plk4 oscillation and the growth of the cartwheel at the proximal end. These findings reveal an unexpected potential crosstalk between factors normally concentrated at opposite ends of the growing centrioles, which might help to coordinate centriole growth.
This article has an associated First Person interview with the first authors of the paper.
Current status and problems of orthopaedic residents in Thailand and Myanmar
Authors: Kasai Yuichi , Paholpak Permsak , Wisanuyotin Taweechok , Sirichativapee Winai , Oo Shwe Kyaw , Thu Si 
Published: June 2022

Background: There is no research about current experiences of orthopaedic residents in Thailand and Myanmar. Methods: A questionnaire survey was distributed among Thai and Myanmar orthopaedic residents to assess their current experiences. This study included a total of 168 participants, comprising 92 orthopaedic residents in Thailand, and 76 in Myanmar who answered the questionnaire. The survey comprised nine questions about issues such as the contents of residency training programs, current training satisfaction, and future careers. The survey was administered anonymously between October 2020 and January 2021. Results: Regarding training content, 24 residents (14.3%) reported being "very satisfied", 103 (61.3%) were "satisfied", 37 (22.0%) were "moderately satisfied", and four (2.4%) were "dissatisfied", and respondents spent a mean of 3.1 h/day reading textbooks and research papers. As for salary, five (3.0%) residents answered "satisfied", 46 (27.4%) responded "moderately satisfied", and 117 (69.6%) were "dissatisfied". Conclusions: Many orthopedic residents in Thailand and Myanmar were enthusiastic about and satisfied with their training. Their only problem was that the salary was low.
Innovative forest products in the circular bioeconomy
Authors: Hassegawa Mariana , Karlberg Anna , Hertzberg Magnus , Verkerk Pieter Johannes 
Published: June 2022

Background: The forest-based industry has been moving towards the manufacture of bio-based products in response to the increasing concern by consumers and governments regarding the use of non-renewable materials and the generation of residues. Various innovative technologies geared towards reducing the environmental footprint of products and processes are currently being developed and applied in the forest-based industry. This study presents some innovative wood-based products that are about to enter the market or that are already being commercialized but have the potential to expand in market size. Methods: We collected data from interviews and a survey with organisations working with product development and manufacturing, and from the literature. Results: Many innovative products that are already produced at an industrial scale, such as cross-laminated timber, wood-based composites, and lyocell, can still increase their market share in the coming years. Some of the up-and-coming products with high potential to substitute fossil-based materials and will likely enter the market in the near future are wood foam, lignin-based adhesives, glycols, bioplastics, and textile fibres. Our study indicates that, although biomass demand is expected to increase, stakeholders do not consider future supply a limiting factor. Conclusions: The ease of market introduction of innovative products relies heavily on the products’ ability to take advantage of existing value chains. Overall, many of the reviewed products have the advantage of being ‘drop-in’. This is because products that require adjustments to production lines are less likely to get into the market without strong external drivers that push for bio-based alternatives. According to stakeholders, the economic viability and the market expansion of these products could be encouraged to a certain extent by EU policies, and certain barriers could be alleviated by reducing bureaucracy, increasing the support for pilot-scale to full-scale production, and increasing subsidies for bio-based alternatives.
New trends in science communication fostering evidence-informed policymaking
Authors: Szüdi Gábor , Bartar Pamela , Weiss Gorazd , Pellegrini Giuseppe , Tulin Marina , Oomen Tessa 
Published: June 2022

This article aims to assess novel trends in science communication relating to how policymakers in the field of innovation and digitalisation policy consume and use scientific findings. We investigate the mutual influence that science communication and policy have on each other and answer the question how emerging science communication trends in the science-policy nexus might influence the use of scientific findings in the policymaking process. By using Google and the largest scholarly repositories, Google Scholar, ResearchGate, and Academia.edu, from 1st March to 31st May 2020, we reviewed policy documents and academic literature containing relevant information on the evolution of characteristics of global, European, and national science communication activities and the interrelated policy responses to identify the most relevant current trends in the evidence-to-policy process alongside three key challenges; trust, translation, and timing. The three identified main trends are (1) a stronger engagement between science and policy, (2) more open, reliable, and accountable science communication practices with policymakers, and (3) the increasing digitalisation and visualisation of science communication. We deepened our investigation by conducting online semi-structured interviews with relevant policy stakeholders at the international and national level between 1st May and 31st July 2020. With the support of the European Commission and building on the existing network of partners, we identified decision-makers and advisors with relevant experience in fields related to innovation and digitalisation policy working in four countries that represent different levels of generalized social trust: Austria, Italy, Hungary, and the Netherlands, and at the international/European Union level. After an online consultation process with a global set of policymakers, these theoretical findings were translated to policy recommendations showcasing possible solutions in science communication that may be initiated, strengthened, or continued by policy stakeholders in order to reach a more effective and efficient uptake of scientific findings in evidence-informed policymaking.
Effect of perceived risk, challenges, and coping on anxiety and depression among frontline health workers during the COVID-19 pandemic in Bangladesh
Authors: Karim Md Rizwanul , Ahmed Helal Uddin , Shaoli Salowa Salam 
Published: June 2022

Background: During the COVID-19 pandemic the health care workers (HCWs) encountered one of the heaviest blows by saving lives while endangering their own. This study was carried out to investigate risk exposures, risk perceptions, challenges, and coping strategies, leading to anxiety and depression, among HCWs in Bangladesh. Methods: This hospital-based cross-sectional survey was conducted from February to July 2021 among 1,112 frontline HCWs. Data were collected using a pre-tested self-administered questionnaire including Patient Health Questionnaire (PHQ-9) and Generalized Anxiety Disorder (GAD-7) scale. A coping scale for COVID-19 (CS-COV-19) was developed and validated. To determine the predictors for symptoms of depression and anxiety, multivariable logistic regression analysis was performed, and the associations between key variables are presented as odds ratios (ORs) and 95% CIs. Results: A total of 54.9% (n=389) doctors and 51.4% (n= 207) nurses were found to have mild to severe anxiety status and 47% (n=333) doctors and 39.9% (n= 159) nurses were found to have mild to severe depression. HCWs, family members, neighbors had been affected with COVID-19 and those who experienced extra stresses at work were two times more likely to have anxiety. HCWs who had worried family and friends that they might get infected through them, confronted any negative social attitude or perceptions, decreased monthly income, and depression were 1.57 to 1.8 times more likely to experience anxiety than those who did not go through these experiences. Health workers who confronted negative social attitudes or social perceptions were 1.7 times more likely to have depression. Respondents, who had family members infected with COVID-19 and HCWs who worked for long hours at hospitals were almost two times more likely to have depression. Conclusions: The findings will help promoting advocacy and encourage implementing selective interventions to protect the mental health and wellbeing of HCWs working with COVID-19.
Genomic landscape of pathogenic mutation of APC, KRAS, TP53, PIK3CA, and MLH1 in Indonesian colorectal cancer
Authors: Marbun Vania Myralda Giamour , Erlina Linda , Lalisang Toar Jean Maurice 
Published: June 2022

Background
Colorectal cancer (CRC) needs several mutations to occur in various genes, and can vary widely in different individuals; hence it is essential to be discovered in a specific population. Until recently, there has been no known study describing APC, TP53, PIK3CA, KRAS, and MLH1 of CRC in Indonesian population. This study describes the nature and location of mutation in CRC patients treated at three different hospitals in Jakarta.

Methods
This descriptive study was conducted on CRC patients who underwent neoadjuvant, surgical, and adjuvant therapy at RSCM, RSKJ, and MRCCC in 2017–2018. DNA analysis was performed using next-generation sequencing and aligned against GRCh38. The pathogenic variant was identified using ACMG classification and FATHMM score. Data related to behavior and survival were collected from medical records.

Results
Twenty-two subjects in which APC, TP53, and PIKCA were mutated. KRAS mutation occurred in 64%, while MLH1 in 45%. There were five mutation types: nonsense, missense, frameshift, splice-site, and silent mutation. There are four groups of co-occurring mutations: APC, TP53, PIK3CA (triple mutation/TM) alone; TM+KRAS; TM+MLH1; and TM+KRAS+MLH1, presenting different nature and survival.

Conclusion
Indonesia has a distinct profile of pathogenic mutation, mainly presenting with locally-advanced stage with various outcomes and survival rate.
Impact of COVID-19 on gynecologic and obstetrical services at two large health systems
Authors: Liang Angela L. , Turner Lindsay C. , Voegtline Kristen M. , Olson Sarah B. , Wildey Brian , Handa Victoria L. 
Published: June 2022

Background
The COVID-19 pandemic disrupted medical care in the US, leading to a significant drop in utilization of some types of health services. We sought to quantify how the pandemic influenced obstetrics and gynecology care at two large health care organizations.

Materials and methods
Comparing 2020 to 2019, we quantified changes to obstetrics and gynecology care at two large health care organizations in the United States, Allegheny Health Network (in western Pennsylvania) and Johns Hopkins University (in Maryland). The analysis considered the numbers of surgical encounters, in-person visits, and telemedicine visits. For each system, we quantified temporal changes in surgical volume, in-person and telemedicine visits, and financial impact related to professional fee revenues. We used segmented regression to evaluate longitudinal effects.

Results
At both institutions, the volume of care was similar in the first few months of 2020 compared to 2019 but dropped precipitously in March 2020. From April to June 2020, surgical volumes were 67% of the same period in 2019 at Allegheny Health and 48% of the same period in 2019 at Johns Hopkins. During that same interval, televisits accounted for approximately 21% of all ambulatory care at both institutions. Although surgical and ambulatory volumes recovered in the second half of 2020, annual surgical volumes in 2020 were significantly lower than 2019 at both institutions (p<0.05) and 2020 ambulatory volumes remained significantly lower at Johns Hopkins (p = .0006). Overall, revenues in 2020 were 91% of 2019 revenues for both institutions.

Conclusions
Obstetrical and gynecologic ambulatory visits and gynecologic surgeries were sharply reduced during the COVID-19 pandemic. Although care volumes returned to 2019 levels in late 2020, we observed an overall reduction in the volume of care provided and a 9% reduction in professional revenue for both institutions.
Exploring the perspectives of key stakeholders on the design and delivery of a cognitive rehabilitation intervention for people post-stroke
Authors: O’ Donoghue Mairéad , Boland Pauline , Leahy Siobhan , Galvin Rose , McManus John , Lisiecka Dominika , Hayes Sara 
Published: June 2022

PurposeStroke is a leading cause of death and disability worldwide. Despite the prevalence and associated burden of post-stroke cognitive impairment, there is uncertainty regarding optimum interventions to improve cognitive function in people post-stroke. The aim of this study is to explore the perspectives of key stakeholders on the design and development of a multidisciplinary intervention to rehabilitate cognitive deficits in people post-stroke.Materials and methodsAudio-recorded, semi-structured interviews were employed with people post-stroke, caregivers, healthcare professionals and academics. All transcribed interviews were exported to NVivo software and analysed using reflexive thematic analysis.ResultsThirty interviews were conducted across stakeholder groups including people post-stroke (n = 10), caregivers (n = 5), healthcare professionals (n = 14) and academics (n = 1). Four themes relevant to the design and development of the intervention were identified (i) engagement in the intervention must be meaningful, (ii) the point of readiness to engage, (iii) a familiar but flexible setting is key (iv) pragmatics of intervention delivery.ConclusionsThese findings present new perspectives across stakeholder groups on the design and delivery of an intervention to rehabilitate cognitive deficits in people post-stroke. Taken together with existing quantitative evidence, these findings will inform the development of a feasibility trial, examining patient and process outcomes, to rehabilitate cognitive deficits post-stroke.
Successful outcomes with low–threshold intervention for cannabis use disorders in Norway - an observational study
Authors: Vederhus John-Kåre , Rørendal Malin , Skårdal Madelene , Næss Marianne Otterstad , Clausen Thomas , Kristensen Øistein 
Published: June 2022

Aims
Cannabis is the most commonly used regulated drug by European youths. Yet, few cannabis-specific interventions have been examined in Europe. The Cannabis Cessation Program (CCP) was developed in Sweden in the 1990s and has been implemented in some Norwegian municipalities. The present study aimed to examine outcomes of this intervention in the Norwegian setting.

Method
The respondents (N = 102) were recruited in four community-based CCPs in Norway. We examined their changes in cannabis use, other substance use, mental distress, well-being, sense of coherence (SoC), and social networks, from baseline (T0) to post-treatment (T1) and up to a 3-month follow-up period (T2). Changes were evaluated with pair-wise t-tests.

Result
Seventy-six participants (75%) completed the 8-week program, according to plan. All participants reported a significant reduction in cannabis use at T1 (average reduction ~16 days per month) and at T2 (N = 59; ~13 days per month). Among those that completed the program, 67% was abstinent from cannabis at T1 and 37% was abstinent at T2. An intention-to-treat analysis showed that 50% (51/102) and 22% (22/102) were abstinent from cannabis use at T1 and T2, respectively. In parallel to abstinence, we observed a substantial reduction in mental distress and an increase in well-being and SoC. Respondents socialized with fewer friends with current substance use, but drug-free social networks were not expanded.

Conclusion
Our findings suggested that the CCP was a valuable, low-threshold manual-based intervention for cannabis use disorders. It showed considerable potential for reducing individuals’ cannabis use.

Clinical trial registration
Clinicaltrials.gov no. NCT04989205. Registered 12 July 2021, i.e., the study was retrospectively registered.
Clinical study of ultrasonic evaluation of T/N staging of differentiated thyroid carcinoma using AJCC 8th staging criteria
Authors: Liang Yu , Huang Xingxiang , Song Zhe , Yang Yang , Lei Ju , Ren Mei , Tan Li , Zhang Hui 
Published: June 2022

Objective
To explore the value of ultrasound in evaluating T/N staging of differentiated thyroid carcinoma (DTC).

Methods
The clinical data of 1206 patients with DTC in our hospital from January 2018 to December 2020 were retrospectively analyzed. Cervical ultrasound was performed before surgery, and the standard ultrasound images of thyroid nodules and cervical lymph nodes I to VII were retained. According to the 8th TNM staging guidelines of AJCC DTC, the T/N stages were assessed by preoperative ultrasonic data. Then, the sensitivity, specificity, negative predicted value, positive predicted value (PPV), and diagnostic value of ultrasound T/N staging were assessed using postoperative pathological staging as the reference.

Results
Ultrasonic T-stage had good consistency to pathological T stage in T4a and T4b tumors (kappa value>0.75), and moderate consistency to pathological T stage in T1, T2 and T3a tumors (kappa value between 0.4 and 0.75). ultrasonic T-stage had a sensitivity higher than 66%, except in T3b assessment (13/44, 29.5%, 95%CI: 16.1%-43.0%). All ultrasonic T-stage had specificity higher than 93%, except in T1b assessment (734/889, 82.6%, 95%CI: 80.1%-85.1%). The PPV of ultrasonic T1a to T4b was 94.3% (494/524), 61.0% (242/397), 54.4% (87/160), 34.3% (12/35), 20.3% (13/64), 100% (22/22) and 100% (4/4), respectively. The diagnostic accuracy values were 83% in T1a, 81% in T1b, 91% in T2, 98% in T3a, 93% in T3b, 99% in T4a and 100% in T4b. Nltrasonic N-stage had poor consistency to pathological N stage in any N stages (kappa value<0.3). The PPV of ultrasonic N0, N1, N1a and N1b was 61.0% (542/889), 55.2% (37/67), 48.2% (53/110), and 24.3% (34/140), respectively.

Conclusion
Ultrasound has a good consistency and high accuracy in assessing the T-stage of DTC. However, the consistency and accuracy were poor in N-staging. It has a certain reference value in reducing excessive surgical treatment of DTC.