bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of An Immune-Related Biomarker Model Based on the CeRNA Network for Triple-Negative Breast Cancer
Authors: wang wenpei , Liu Xing , zhou xiang , xiao yu , liu hongguang 
Published: November 2022

Abstract
Background
Triple-negative breast cancer (TNBC) accounts for 10–20% of all breast cancer cases. It represents an aggressive malignancy of frequently high histologic grade with no effective specific targeted therapies. Numerous studies have indicated that abnormal expressions of circRNAs may be a potential mechanism for the development and progression of cancer. CircRNAs may bind with miRNAs to influence the interaction between miRNAs and mRNAs and contribute to the progression of tumors, which was called as competitive endogenous RNA (ceRNA) hypothesis. In this study, we aimed to establish the ceRNA network in TNBC, and identify immune-related genes.
Methods
The circRNA (GSE101123), microRNA (TCGA-TNBC), and mRNA (TCGA-TNBC) expression profiles of TNBC were collected from the GEO database and TCGA database. Immune-related genes were collected from the ImmPort database. Differentially expressed circRNAs, miRNAs and mRNAs were identified. Then, the interacting relationships among circRNA-miRNA and miRNA-mRNA were predicted, and we constructed an immune-related ceRNA network. Subsequently, an immune-related signature was developed based on mRNAs in the ceRNA network.
Results
A total of 24 DEcircRNAs, 45 DEmiRNAs, 4814 DEmRNAs were identified. After overlapping DEmRNAs with immune-related genes, we obtained 490 immune-related DEmRNAs (DEIRGs). Then we took the intersection of 490 DEIRGs and 407 predicted miRNAs to obtain 5 immune-related mRNAs (IRGs). GO and KEGG pathway analysis showed that IRGs were associated with immunity. Then, an immune-related ceRNA network was constructed. Among the IRGs in ceRNA network, the AUC curve indicated that ADCYAP1R1, ANGPTL2, IL2RA and TGFBR2 with significantly diagnosis value were identified. Finally, we analyzed the correlation between 4 hub genes and immune infiltrating cells, ADCYAP1R1 were negatively associated with Type 2 T helper cell.
Conclusion
We screened the key genes and established an immune-related ceRNA network involved in TNBC, which will assist in understanding the molecular mechanisms underlying the carcinogenesis and progression.
The effect of surface treatment of zirconia with hydrofluoric acid in high concentrations on surface resistance bond: an in vitro study
Authors: de Oliveira Leonardo José Rodrigues , Júnior Eneas Pereira da Silva , Nascimento Ângela , Cavalcanti Maria Hermínia Aníbal , Júnior Rodivan Braz Silva , Silva Cláudia Geisa Souza e , Correia Tereza Cristina , Soares Natália Karolina Vieira , Braz Rodivan Braz 
Published: November 2022

Abstract
Objectives

This study aimed to evaluate whether conditioning with hydrofluoric acid in higher elevations with a longer application time can promote an increase in surface roughness and shear bond strength to zirconia.

Methods

Fifty zirconia cylinders (Vipiblock- Zinconn- Vipi-Brazil) were made using CAD / CAM system and cemented with Panavia F 2.0 resin cement (Kuraray-Japan) in composite resin cylinders filtek Z-350 XT (3M / ESPE-EUA), after different surface treatments of zirconia (n = 10): Group 1 - No surface treatment; Group 2 - Conditioning with 40% hydrofluoric acid, for 2 minutes; Group 3 - Conditioning with 40% hydrofluoric acid, for 4 minutes, Group 4 - Conditioning with 50% hydrofluoric acid, for 2 minutes and Group 5 - Conditioning with 50% hydrofluoric acid, for 4 minutes. Right after cementation, all specimens were subjected tothermocycling (5000 cycles; 5º to 55ºC), storaged in distilled water at 37ºC for 730 days and subjected to the shear bond strength test in a universal testing machine until fracture. The F test (ANOVA) for one factor and the multiple comparison tests of Tamanhe were used (p < 0,05).

Results

The highest mean corresponded to group 3 (4.47 Mpa), followed by group 5 (4.05 Mpa), Group 4 (3.28 Mpa) and group 2 (3.14 Mpa).
Significance
Conditioning with 40% hydrofluoric acid for 4 minutes obtained the highest values of bond strength to zirconia, however there was no statistically significant difference in relation to the concentration of 50%, for 2 and 4 minutes.
Ultra-efficient generation of time-energy entangled photon pairs in a InGaP Photonic Crystal Cavity
Authors: Chopin Alexandre , Barone Andrea , Ghorbel Ines , Combrié Sylvain , Bajoni Daniele , Raineri Fabrice , Galli Matteo , De Rossi Alfredo 
Published: November 2022

Abstract
Time-energy entangled photon pairs and heralded single photons are generated in a 25 μm-long, In0.5Ga0.5P Photonic Crystal cavity via degenerate Spontaneous Four-Wave-Mixing with a very large efficiency (16 GHz/mW2), owing to the very small confinement volume. Maximal efficiency is reached via thermal tuning, which compensates for the frequency mismatch of the cavity modes. A large off-chip coincidence rate (70 kHz) is measured with pump power ≈ 36 μW. Time-energy entanglement is measured with raw visibility up to 93.88 % using 1 second integration time constant. This qualifies photonic crystal cavities as sources for quantum photonics.
Pragmatic Cluster Randomized Trial to Evaluate Effectiveness and Implementation of Ehr-facilitated Collaborative Symptom Control in Cancer (E2c2): Addendum
Authors: Herrin Jeph , Rutten Lila J.Finney , Ruddy Kathryn J. , Kroenke Kurt , Cheville Andrea L. 
Published: November 2022

Abstract
We previously described the hypotheses, outcomes, design, and analysis for E2C2, a pragmatic stepped-wedge trial to assess an intervention to improve symptom control in patients with cancer. Subsequent consideration of the design and cohort led to the addition of a second primary hypothesis. This article describes and presents the rationale for this second hypothesis. This addendum also details a revised analytic approach, necessitated by inconsistencies in the original analytic plan. The design, outcomes, and other aspects of the protocol remain unchanged.
Epicardial Fat Thickness in Patients with Acute Retinal Vein Occlusion
Authors: Coşansu Kahraman , özmen Sedat , Vatan Mehmet Bülent 
Published: November 2022

Abstract
Background: Retinal vein occlusion (RVO) is one of the most frequently occurring retinal vascular disorders. Epicardial fat thickness (EFT) has been associated with thromboembolic diseases, including atherosclerotic and neurovascular diseases. The relationship between EFT and RVO has not yet been explored. The aim of this study was to evaluate whether EFT is a marker for acute central RVO.
Methods: A total of 64 acute central RVO patients that referred from ophthalmologist for routine cardiac check and 64 age-gender matched controls were included in the study. Echocardiographic measurement of EFT was recorded between August 2021 and May 2022 in Sakarya University Education and Research Hospital according to previously published methods.
Results: In comparison with the control group, the statistically significant difference was found with regard to interventricular septal (p= .002) and posterior wall thickness (p= .005), left atrial diameter (p= .001), and aortic root diameter (p= .003). Moreover, acute central RVO patients had significantly higher epicardial fat thickness (7.04 ± 1.47 vs 4.20 ± 0.71 mm; P &lt;0.0001). The multivariate regression analysis indicated that EFT and statin usage are independently predictors of RVO.
Conclusions: The present study showed that, EFT and statin usage are independent predictors for the development of acute RVO.
The Outcome of IV Vitamin C Therapy in Patients with Sepsis or Septic Shock: A Meta-Analysis of Randomized Controlled Trials
Authors: Liang Baofang , Su Jianwei , Shao Hanquan , Chen Huiying , Xie Baocheng 
Published: November 2022

Abstract
Background: To update a meta-analysis of randomized controlled trials (RCTs) and further explore the outcome of IV vitamin C (IVVC) administration in sepsis or septic shock patients.
Methods: This study is a meta-analysis of RCTs. The RCTs of vitamin C therapy in sepsis were searched in PubMed, EMBASE and Clinical Trials.gov since August 16, 2022. We registered the protocol with PROSPERO (CRD42022354875). The primary outcome was mortality included 28-day, 30-day, or in hospital mortality. Two reviewers independently assessed RCTs according to eligibility criteria: 1) Study type: RCT; 2) patient population: patients ≥18 years with sepsis or septic shock; 3) intervention: IVVC at any doses as monotherapy or combined with thiamine or and hydrocortisone compared with standard of care, no intervention, or placebo (defined as control group) ; 4) the RCT described primary outcome. Then, two authors independently extracted related information from RCTs.
Results: Eighteen RCTs (n=2980 patients) were identified in this meta-analysis. Treatment with IVVC was not shown to improve mortality (odds ratio,0.87; 95% CI, 0.75–1.02; p=0.09; I2=44%) regardless of different dose or type of therapy, whereas there was significant effects in duration of vasopressor use (MD, –15.31; 95% CI, –21.92 to –8.69; p<0.00001; I2 =59%) and change in the Sequential Organ Failure Assessment (SOFA) score from baseline to 72–96 hours (MD, –0.64; 95% CI, –1.15 to –0.13; p=0.01; I2=65%), and was relatively safe in sepsis or septic shock patients (OR, 1.22; 95% CI, 0.98–1.51; p=0.08; I2=40%).
Conclusion: In this meta-analysis, IVVC in sepsis or septic shock patients was relatively safe and significantly shorten the duration of vasopressor use and improved the change of SOFA score, whereas it was not associated with reduction in mortality.
FGF21-FGFR1 controls mitochondrial homeostasis in cardiomyocytes by modulating the degradation of OPA1
Authors: Han Yaling , Yan Bing , Mei Zhu , Tang Yaohan , Song Haixu , Wu Hanlin , Zhang Xiaolin , Yan Chenghui 
Published: November 2022

Abstract

Fibroblast growth factor 21 (FGF21) is a pleiotropic hormone, secreted mainly by the liver, considered as a major regulator of energy homeostasis. Recent research revealed that FGF21 could play an important role in cardiac pathological remodeling effects and preventing cardiomyopathy, but the underlying mechanism remained largely unknown. The aim of this study was to clarify the mechanism of FGF21 cardiac protective effects. We engineered FGF21 knock out mice, and the effects of FGF21 and its downstream mediators were subsequently elucidated using western blot, qRT-PCR, and mitochondrial morphological, functional analysis. FGF21 knock out mice resulted in cardiac dysfunction accompanied by a decline in global longitudinal strain (GLS) and ejection fraction (EF) which was independently of obesity. Mitochondrial quality, quantity and functions were abnormal accompanied with the decreased levels of optic atrophy-1 (OPA1) in FGF21 KO mice. In contrast to FGF21 knockdown, the cardiac specific overexpression of FGF21 can alleviate cardiac dysfunction caused by FGF21 deficiency. In vitro study FGF21 siRNA can deteriorate mitochondrial dynamics, functions impairment induced by CoCl2. Both recombinant FGF21 and adeno virus mediated FGF21 overexpression can alleviate CoCl2 induced mitochondrial impairment by restoring mitochondrial dynamics. FGF21 is essential for maintaining mitochondrial dynamics and functions in cardiomyocytes. FGF21, as an important target in regulating cardiomyocytes mitochondrial homeostasis under oxidative stress, will provide new therapeutic options for heart failure patients.
Climate change and human footprint increase insect defoliation across central boreal forests of Canada
Authors: Kalamandeen Michelle , Gulamhussein Imran , Castro José Bermúdez , Sothe Camile , Gonsamo Alemu 
Published: November 2022

Abstract
Anthropogenic climate change is contributing to increased insect infestation globally, leading to pest population growth, expansion of niche and geographic range as well as increased outbreak frequencies, resulting in economic losses and reduction in food security. In recent years, spongy moth (Lymantria dispar dispar), jack pine budworm (Choristoneura pinus pinus), large aspen tortrix (Choristoneura conflictana) and spruce budworm (Choristoneura fumiferana) caused widespread defoliation across one of Canada’s most forested provinces, Ontario. Such outbreaks have been limited to field sightings around Ontario, with relatively few studies exploring potential anthropic and climate drivers of infestation. Using random forest probability estimates and satellite data downscaled to 1km spatial resolution from the Moderate Resolution Imaging Spectroradiometer (MODIS), we reveal considerable expansion of insect defoliation across Ontario between 2018 and 2020 than previously recorded. High probability of infestation grew by 1300% from 2018 to 2020, an area expansion of ~40,000 km2 in the northwest and southern parts of the province. Much of the geographic expansion of outbreaks was driven by increasing temperature, and for the first time, linked to the proximity to roads. This expansion presents an important and alarming new challenge for forest conservation and management in Ontario, in particular, and Canada in general.
The clinical efficacy of a new surgical technique of oral mucosal epithelial transplantation for severe ocular surface disorders
Authors: Zhu Yuanfang , Qiu Wenya , Xu Yesheng , Yao Yufeng 
Published: November 2022

Abstract
Background
Sever ocular surface disorder was one of the main blinding diseases. Reconstruction work for this kind of ocular surfaces is difficult due to the lack of original tissues. We developed a new surgical technique to reconstruct the ocular surface by using oral mucosal epithelia as the alternative for the limbus in such kind of eyes since 2011, which we called it oral mucosal epithelial transplantation (OMET). The study was designed to analyze the clinical efficacy for OMET in reconstruction of eyes with severe ocular surface disorder.
Methods
Retrospective review of patients with severe ocular surface disorder who underwent OMET during 2011 to 2021 at the Department of Ophthalmology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine. Patients who had been followed up for at least 3 months after surgery and had sufficient pre- or postoperative records were included. Surgical efficacy was evaluated by comparing the following pre- and postoperative factors: best-corrected visual acuity, corneal transparency, neovascularization grade and symblepharon grade. Post-operative ocular surface impression cytology results were analyzed to study the morphology of the new corneal epithelial cells.
Results
Forty-eight patients (49 eyes) with a mean age of 42.55 ± 12.40 years (range, 12–66 years) were enrolled. Injuries included chemical burns (30 eyes), thermal burns (16 eyes), explosive injuries (1 eye), Stevens-Johnson syndrome (1 eye), and multiple pterygiums (1 eye). The mean follow-up period was 25.97 ± 22.99 months (range, 3–94 months). After surgery, 29 eyes (59.18%) had improved corneal transparency, and 26 eyes (53.06%) had improved best-corrected visual acuity. Forty-seven eyes (95.92%) had a stable epithelium, with a mean epithelization time of 26.04 ± 26.43 days. Forty-four eyes (89.80%) had a clearly reduced degree of neovascularization. Of 20 eyes with preoperative symblepharon, 15 (75%) were completely corrected, and 5 (25%) were partially corrected. Impression cytological studies showed that the newly transdifferentiated epithelial cells were morphologically different from the corneal or conjunctival epithelial cells and more like the oral mucosal epithelial cells of their origin.
Conclusions
OMET was an effective surgical technique for severe ocular surface disorder reconstruction.
Knowledge attitudes and practices of healthcare professionals on oral care of pregnant women- a national study
Authors: Muhammad-Safwan Nur-Afifah Syafiqah , Abdul-Mumin Khadizah H. , Rahman Hanif Abdul , Gharif Rafidah , Haji-Momin Haji-Mohin , Kisut Ramlah , Zielinski Ruth , Dhaliwal Jagjit Singh 
Published: November 2022

Abstract

Objective
To examine nationwide the knowledge, attitudes and practices of healthcare professionals on the oral care of pregnant women in Brunei Darussalam.
Materials and methods
A descriptive cross-sectional study conducted using an online survey on eligible healthcare professionals in all Government Maternal and Child Health Care Clinics, Dental Clinics and Obstetrics and Gynecological Clinics throughout Brunei Darussalam (N = 346).
Results
A total of 346 healthcare professionals participated in this online study. Participants’ view on the importance of dental/oral health in prenatal/antenatal care, most (94.3%) responded that they perceive dental/oral health to be of high importance. Though, only a little over half of the participants (59.0%) included dental/oral health questions during prenatal/antenatal health assessments. Moreover, only 16.2% of participants frequently discussed regarding the importance of dental/oral care with their prenatal/antenatal patients.
Conclusions
According to this study, most healthcare professionals had a positive attitude towards the importance of dental/oral care for prenatal/antenatal patients. However, not all portrayed it when it comes to their practice with regard to dental/oral care in pregnant women.
Acaricide Residues in Beeswax. Implications in Honey, Brood and Honeybee and Influence of Landscape Around Colonies
Authors: Albero Beatriz , Miguel Esther , García-Valcárcel Ana I. 
Published: November 2022

Abstract
To make beekeeping sustainable, the management of bee colonies to produce bee products financially viable without compromising the life of bees must implement acceptable practices such as the treatment of hives with appropriate products. Occasionally, the use of acaricides to treat the hives against varroosis is uncontrolled and can accumulate in the hives endangering the bee colonies. In this work, a screening of seven acaricides was carried out in different apiaries in Andalusia (Spain). Their distribution in beeswax, brood, honey, and bee, was evaluated in different times considering the influence of the environment (agricultural, urban and forest) surrounding the colonies. It was found that beeswax was highly polluted but honey, brood and bees had acceptable levels, below their respective MRL or LD50, past a certain period after varrocide treatments. The landscape management around the hives treated with products against Varroa does not influence the hive contamination. Acaricides banned for their use against Varroa, such as chlorfenvinphos, cypermethrin and especially acrinathrin, were found in the hives analysed.
The carbohydrate-binding module mediates mCherry protein anchoring on the cell wall in rice
Authors: Xiao Guoying 
Published: November 2022

Abstract
The red fluorescent protein mCherry is widely used as a tagged protein in intracellular protein positioning and dynamic tracing due to its stable characteristics in color and monomer molecule. In this study, mCherry was used as a tag to explore the cell wall-directed binding of the carbohydrate-binding module (CBM). The results of fluorescent signal detection showed that both the ubiquitin promoter and the CaMV35S promoter could drive the expression of mCherry gene in seeds, leaves, and roots. The results of subcellular localization by different methods displayed that the fluorescence signals were concentrated on the cell walls of the root, stem, leaf, and vascular bundle sheath. It indicated that the CBM could mediate the red fluorescent protein anchoring on the cell wall.
Measurement and Analysis of Maxillary Anterior Teeth Color In The Chinese Population
Authors: Ruan Chenglu , Wang Lin 
Published: November 2022

AbstractObjectiveTo measure the difference in the crown color of the maxillary anterior teeth in the Chinese population, to study its potential regularity, and to provide a reference for the colorimetry of oral anterior teeth restoration.MethodUse VITA Easyshade Advance4.0 spectrophotometer to measure the color of 1/3 of maxillary anterior crowns in different age groups, use CIE-1976-L*a*b* color system to describe the color, use Spss26.0 software Statistical analysis was performed to obtain the trend of the color change of maxillary anterior teeth.Conclusion:1. The color of the labial crown of maxillary anterior teeth in the Chinese population is related to different age groups and tooth positions but not gender. 2. In the Chinese population, the color of the maxillary anterior teeth on the labial side gradually decreased from the central incisor to the distal end of the dental arch while the chroma gradually increased. 3. With age increase, the L* and a* values ​​of the upper central incisors, upper lateral incisors, and upper canines gradually decrease, and the b* value gradually increases. The teeth became darker, more yellow, and redder.
The association of KIR locus with breast cancer risk in Kermanshahi women population
Authors: Shahrokhvand Seyedeh Zahra , Payandeh Mehrdad , Samimi Zahra , Feizollahi Parisa , Ghobadinezhad Farbod , Taghadosi Mahdi , Nikjo Payam 
Published: November 2022

Abstract
Killer cell immunoglobulin-like receptors (KIRs) regulate the antitumor effect of Natural killer cells. This study aims to compare the frequency of KIR genes distribution in women with breast cancer and the control group in Kermanshah province. This study was performed on 53 women with BC and 37 healthy women. The KIR gene content was determined by polymerase chain reaction with sequence-specific primers (PCR-SSP). The frequency of the KIR- 2DL5B gene was significantly different between the two groups (P: 0.037), and this locus increases the risk of disease (OR: 2.491). The following results were associated with breast cancer risk: the cBx-tAtA distribution (OR: 5.122), the B content score 1 (OR: 5.122), tA01|tA01 (OR: 5.122) and inversely the following results were associated with protection: the cBx-tBx distribution (OR: 0.176), the B content score 2 (OR: 0.176), cA01|cB0X (OR: 0.287) and tA01|tB0X (OR: 0.301). These results suggested that KIR- 2DL5B, the cBx-tAtA, the B content score 1 and tA01|tA01 were associated with increased susceptibility while the cBx-tBx, the B content score 2, cA01|cB0X, and tA01|tB0X were associated with protection for BC in kermanshahi women population.
GATS Tag System for Protein Analysis with Biotin Labelling Methods
Authors: Yamada Kohdai , Soga Fumiya , Tokunaga Soh , Nagaoka Hikaru , Ozawa Tatsuhiko , Kishi Hiroyuki , Takashima Eizo , Sawasaki Tatsuya 
Published: November 2022

Abstract
Polypeptide tags and biotin labelling technologies are widely used for protein analyses in biochemistry and cell biology. Although biotin labelling reacts with a lysine residue, many peptide tag epitopes have lysine residue(s). Here, we propose the GATS tag system without a lysine residue and with high sensitivity and low non-specific binding using a rabbit monoclonal antibody against Plasmodium falciparum glycosylphosphatidylinositol (GPI)- anchored micronemal antigen (PfGAMA). From 14 monoclonal clones, an Ra3 clone was selected as it recognized an epitope—TLSVGVQNTF—without a lysine residue; this antibody and epitope tag set was called the GATS tag system. Surface plasmon resonance analysis showed that the tag system had a high affinity of 8.71 × 10-9 M. GATS tag indicated a very low background with remarkably high sensitivity and specificity in immunoblotting using the lysates of mammalian cells. It also showed a high sensitivity for immunoprecipitation and immunostaining of cultured human cells. The tag system was highly sensitive in both biotin labelling methods for proteins using NHS-Sulfo-biotin and BioID (proximity-dependent biotin identification) in the human cells, as opposed to a commercially available tag system having lysine residues, which showed reduced sensitivity. These results showed that the GATS tag system is suitable for methods such as BioID involving labelling lysine residues.