bluuebunny/crossref_metadata_2025_split huggingface dataset:
Yeast display platform for expression of linear peptide epitopes to assess peptide-MHC-II binding in high-throughput
Authors: Huisman Brooke D. , Balivada Pallavi A. , Birnbaum Michael E. 
Published: August 2022

AbstractYeast display can serve as a powerful tool to assess peptide-MHC (pMHC) and pMHC-TCR binding. However, this approach is often limited by the need to optimize MHC proteins for yeast surface expression, which can be laborious and may not yield productive results. Here we present a second-generation yeast display platform for class II MHC molecules (MHC-II) which decouples MHC-II expression from yeast-expressed peptides, referred to as “peptide display”. Peptide display obviates the need for yeast-specific MHC optimizations and increases the scale of MHC-II alleles available for use in yeast display screens. Because MHC identity is separated from the peptide library, a further benefit of this platform is the ability to assess a single library of peptides against any MHC-II. We demonstrate the utility of the peptide display platform across MHC-II proteins, screening HLA-DR, HLA-DP, and HLA-DQ alleles. We further explore parameters of selections, including reagent dependencies, MHC avidity, and use of competitor peptides. This approach presents an advance in throughput and accessibility of screening peptide-MHC-II binding.
Sex-linked markers in an Australian frog Platyplectrum ornatum with a small genome and homomorphic sex chromosomes
Authors: Schimeck Chad , Shams Foyez , Miura Ikuo , Clulow Simon , Majtanova Zuzana , Deakin Janine , Ezaz Tariq 
Published: August 2022

AbstractAmphibians have highly diverse sex-determining modes leading to a notable interest in vertebrate sex determination and sex chromosome evolution. The identification of sex-determining systems in amphibians, however, is often difficult as a vast majority consist of homomorphic sex chromosomes making them hard to distinguish. In this study, we used Diversity Array Technology sequencing (DArTseq™) to identify the sex-determining system in the ornate burrowing frog from Australia, Platyplectrum ornatum. We applied DArTseq™ to 44 individuals, 19 males and 25 females, collected from two locations to develop sex-linked markers. Unexpectedly, these 44 individuals were classified into two distinct population clusters based on our SNP analyses, 36 individuals in cluster-1, and 8 individuals in cluster-2. We then performed sex-linkage analyses separately in each cluster. We identified 35 sex-linked markers from cluster-1, which were all associated with maleness. Therefore, P. ornatum cluster-1 is utilising a male heterogametic (XX/XY) sex-determining system. On the other hand, we identified 210 sex-linked markers from cluster-2, of which 89 were male specific, i.e., identifying XX/XY sex determining system and 111 were female specific, i.e., identifying ZZ/ZW sex determining system, suggesting existence of either male or female heterogametic sex determining system in cluster-2. We also performed cytogenetic analyses in 1 male and 1 female from cluster-1; however, we did not detect any visible differentiation between the X and Y sex chromosomes. We also mapped sex-linked markers from the two clusters against the P. ornatum genome and our comparative analysis indicated that the sex chromosomes in both clusters shared homologies to chromosome 10 (autosome) of Rana temporaria and ZWY sex chromosome of Xenopus tropicalis. It is plausible that the cluster-2 has a potential to be either male or female heterogamety in sex determination, requiring further investigation.
The adaptation dynamics between copy-number and point mutations
Authors: Tomanek Isabella , Guet Calin C. 
Published: August 2022

AbstractCopy-number and point mutations form the basis for most evolutionary novelty through the process of gene duplication and divergence. While a plethora of genomic sequence data reveals the long-term fate of diverging coding sequences and their cis-regulatory elements, little is known about the early dynamics around the duplication event itself. In microorganisms, selection for increased gene expression often drives the expansion of gene copy-number mutations, which serves as a crude adaptation, prior to divergence through refining point mutations. Using a simple synthetic genetic system that allows us to distinguish copy-number and point mutations, we study their early and transient adaptive dynamics in real-time in Escherichia coli. We find two qualitatively different routes of adaptation depending on the level of functional improvement selected for: In conditions of high gene expression demand, the two types of mutations occur as a combination. Under low gene expression demand, negative epistasis between the two types of mutations renders them mutually exclusive. Thus, owing to their higher frequency, adaptation is dominated by copy-number mutations. Ultimately, due to high rates of reversal and pleiotropic cost, copy-number mutations may not only serve as a crude and transient adaptation, but also constrain sequence divergence over evolutionary time scales.
Adhesion of auto-mixed and manual-mixed self-adhesive resin cements to dentin on exposure to long-term high-altitude pressure
Authors: Ozkan Ata Secil , Akay Canan , Mumcu Emre , Ata Nazim 
Published: August 2022

PurposeThe purpose of this study is to test the shear-bond-strengths of auto-mixed and manual-mixed self-adhesive resin cement to dentin on long-term high-altitude pressure.Design/methodology/approachHuman molars were embedded in acrylic resin. Sixty composite resin discs were obtained. The composite resin discs were bonded to dentin using hand-mixed and auto-mixed self-adhesive resin cement. After cementation, the samples were stored in artificial saliva and divided into two subgroups (n= 30), hypobaric pressure and the atmospheric pressure group. The specimen underwent three pressure cycles per day for 100 days. The failure types were evaluated after debonding with scanning electron microscopy. The shear bond strength was tested with Universal Testing Machine. Analysis of variances/Tukey post hoc tests were used for statistical analysis. Groups were also evaluated by the Weibull modulus.FindingsRegardless of hypobaric pressure changes, the highest bond strength was examined in auto-mixed Panavia SA samples. A significant difference was found in both auto-mixed MaxCem EC and hand-mixed RelyX U200 group after exposure to hypobaric pressure compared to the control group.Originality/valueThe luting cement-type, mixing methods of cements and environmental pressure changes significantly influence the bond strengths. Dentists can use auto-mixed self-adhesive resins in patients likely to be exposed to hypobaric pressure.
The forensic implications of camouflaging: a study into victimisation and offending associated with autism and pathological demand avoidance
Authors: Trundle Grace , Jones Katy A. , Ropar Danielle , Egan Vincent 
Published: August 2022

Purpose
This study aims to investigate the influence of social camouflaging on victimisation and offending in relation to autism and pathological demand avoidance (PDA) traits. Camouflaging aims to overcome or conceal difficulties in social and communication skills. Autistic individuals report camouflaging in response to threat and being verbally and physically assaulted when they have not camouflaged. Thus, camouflaging could be associated with victimisation. Camouflaging could also impact on specialist support available to an individual, potentially increasing the risk of victimisation or offending.

Design/methodology/approach
Cross-sectional study was conducted using 220 participants from the general population who completed online questionnaires measuring victimisation and offending, autism and PDA traits, camouflaging and symptoms of depression and anxiety.

Findings
Correlational analysis found positive associations between camouflaging and victimisation, and camouflaging and lifetime offending. Greater camouflaging and PDA traits predicted greater offending, whereas greater autism traits predicted fewer offending behaviours. While correlated, camouflaging was not significantly predictive of victimisation. Victimisation was predicted by symptoms of depression and PDA traits.

Originality/value
To the best of the authors’ knowledge, this study is the first to consider camouflaging as an influencing factor on offending and victimisation in autistic and PDA individuals.
Human-centric BPM: employee exploitation effects on knowledge-sharing behavior and CWB: a dual-path model and its consequences
Authors: Malik Muhammad Faisal , Burhan Qurat-ul-Ain , Akhtar Shazia , Shafiq Muhammad 
Published: August 2022

PurposeThe research intended to identify the impact of employee exploitation on knowledge-sharing behavior and withdrawal, not in isolation, but by taking psychological ownership and psychological detachment as mediating variables. Moreover, the research aims to identify optimism’s moderating role concerning employee exploitation and psychological ownership. The research aims to suggest the management implementation of the human-centric business process and, subsequently, management to obtain maximum output from employees.Design/methodology/approachPositivism research philosophy followed by a deductive approach is adopted to meet the objectives of the current study. Survey techniques with a self-administrated questionnaire were used to collect data from employees working in public sector organizations. Moreover, 255 employees with the highest qualification were shortlisted to capture the variables’ impact. Data analysis was done by using SEM-AMOS approaches and obtained structural and path models to test the formulated hypotheses.FindingsResults suggested that employee exploitation has a significant relationship with withdrawal, and psychological detachment mediates the relationship. The other path suggested the insignificant role of optimism as a moderator in the relationship between employee exploitation and psychological empowerment. However, psychological empowerment fully mediates the relationship between employee exploitation and knowledge-sharing behavior.Originality/valueA plethora of research is available on employee exploitation; however, the current research is first to capture both positive and negative paths in public sector organizations. It provides clear insights for managers to reformulate and reanalyze their organizational policies to get employees' positive attitudinal and behavioural outcomes.
Contractor selection for green buildings based on the fuzzy Kano model and TOPSIS: a developer satisfaction perspective
Authors: Cai Jin , Li Zhongfu , Dou Yudan , Teng Yue , Yuan Mengqi 
Published: August 2022

PurposeContractor selection is critical in green buildings (GBs) since the preferred contractor has the responsibility to achieve construction sustainability as well as relationship sustainability. The developer satisfaction reflecting requirements can boost the cooperative relationship among stakeholders and act as an evaluation scale for the success of GB projects, which needs to be emphasized in the selection process but little involved in the existing research. This study explores improving GB contractor (GBC) selection by integrating developer satisfaction into selection procedures.Design/methodology/approachA systematic framework of GBC selection including twenty-five criteria from literature review and experts survey is firstly constructed. Both tactical and strategic criteria are further classified into Kano categories (must-be, one-dimensional, and attractive categories) using the fuzzy Kano model (FKM), and weighted by the developer satisfaction index. The model proposed by this study combining FKM and TOPSIS divides the selection process into the filtration phase and selection phase by Kano categories. The proposed model is finally verified through performance comparison among multiple methods in a case.FindingsSelection criteria are measured linearly and nonlinearly, showing criteria having nonlinear satisfaction change accounts for two-thirds of all. Criteria at tactical level tend to be must-be or one-dimensional categories for the developer, and most strategic criteria are classed as the attractive category, indicating that adding strategic criteria is necessary for long-term cooperation. The proposed model, using developer satisfaction to improve the selection process, ensures the selected GBC to be the most satisfactory with requirements of the developer and makes the performance of GBCs easily distinguishable.Originality/valueThis study contributes to the existing body of knowledge for promoting relationship sustainability by supplementing an integrated model with emphasis on developer satisfaction in GBC selection, so as to establish a good initial foundation due to the match between performances of GBCs and needs of developers. It not only helps maximize developer satisfaction in GBC selection by applying satisfaction to pre-construction management, but also instructs GBCs to prioritize performance improvements. The framework is also conducive for developers to classify selection criteria and select other participants (like green suppliers) from the satisfaction perspective in GBs.
Skills audits: an integrative literature review
Authors: McGuire David , McVicar Orla , Tariq Umm E. Habiba 
Published: August 2022

PurposeSkills audits represent an important vehicle for analysing and monitoring skills coverage across organisations and nations and identifying skills gaps and mismatches that exist. In so doing, they provide valuable data on the competitive positioning of organisations and nations and help determine priorities for investment. The purpose of this paper is to examine the use and prevalence of skills audits to improve individual, organisational and societal outcomes.Design/methodology/approachFollowing the guidance of Torraco (2016), this paper takes the form of an integrative literature review. The authors conducted a series of keyword searches using databases such as Google Scholar, ABI Inform, Science Direct and Emerald. Title word searches used the terms: “skills audit”, “skills analysis”, “skills inventory”, “skills mapping” and the authors reviewed articles that were published between the period: 1990 to 2020.FindingsSkills audits remain a useful tool to assess skill attainment and progression on university and college programmes, giving individuals a better understanding of their particular strengths and allowing them to develop realistic career plans and goals. From an organisational perspective, skill audits add value where they are strategically linked to job design, recruitment and training initiatives. They are also useful in helping organisations identify skill shortages, particularly if they relate skills outages to future growth areas. Finally, skills audits can sustain career counselling, but need to remain flexible in categorising skills in relation to the gig economy and remote working.Originality/valueThe topic of skills audits remains an under-researched topic in the training and human resource development literature. To date, limited studies have been carried out examining their use and effectiveness. This paper sheds light on a topic worthy of exploration in the field.
Zoning design of pressure comfort for women's shaping pants based on a new material made by the LRJ40/1F warp-knitting machine
Authors: Yin Wei 
Published: August 2022

PurposeZoning design of pressure comfort of women's shaping pants is studied in this paper based on the new material made by the LRJ40/1F warp-knitting machine.Design/methodology/approachAccording to the distribution of body fat and the pressure comfort range of the shaping pants, zoning design is carried out. The pressure comfort of the women's shaping pants is tested by the objective pressure test and the subjective evaluation method.FindingsThe result is that using the method of zoning design can satisfy the comfort of the lower limbs while improving the mobility and plasticity.Originality/valuePressure comfort has become an important index to evaluate the comfort of elastic shaping pants. However, there were very few studies on the relationship between the pressure comfort of warp-knitted elastic pants and the design of jacquard organization in China and abroad.
Stalking and the role of occupational therapy “you’re not living life to the full if you’re stalking”
Authors: Wheatley Rachael , Baker Sam 
Published: August 2022

Purpose
This discussion paper aims to highlight the role of occupational therapy (OT) in understanding stalking and in interventions designed to assist the perpetrator to lead a more fulfilling life through healthier occupations.

Design/methodology/approach
This study highlights the role of OT in understanding stalking and in designing interventions to assist the perpetrator by extending discussions, drawing on the authors’ practitioner experiences and upon recent study findings on what drives men who stalk.

Findings
Stalking is a problem behaviour that is often effort-intense, all-consuming, emotionally driven and psychologically damaging for both victims and perpetrators. It consists of a patterned occupation of time which is overarchingly dysfunctional, yet intrinsically purposeful. As humans, our actions and occupations have meaning to us. Stalking can be conceptualised as a meaningful yet self-defeating and harmful pattern of occupations. This paper illustrates how stalking could be addressed through the additional contribution of OT to multi-agency approaches.

Research limitations/implications
This paper extends the discussion by drawing on the authors’ clinical practitioner experiences and upon recent study findings on what drives men who stalk.

Practical implications
This paper highlights the role of OT in understanding stalking and in interventions designed to assist the perpetrator to lead a more fulfilling life through healthier occupations.

Originality/value
This marriage of knowledge from OT and stalking research is set out in support of the application of OT within multi-agency approaches to working with people who stalk.
Key performance factors for integration of Industry 4.0 and sustainable supply chains: a perspective of Indian manufacturing industry
Authors: Gopal PRC , Kadari Punitha , Thakkar Jitesh J. , Mawandiya Bimal Kumar 
Published: August 2022

Purpose
The purpose of this paper is to identify the key performance factors that can lead toward sustainability in the Industry 4.0 supply chains of manufacturing industries.

Design/methodology/approach
Questionnaire is used to collect the data from manufacturing sector to prioritize the factors, which integrates both Industry 4.0 and sustainability. For this, stepwise weight assessment ratio analysis (SWARA) method is used to obtain the weights for criteria and sub-criteria to prioritize the factors.

Findings
The present study brings the findings about five key performance factors. Social factor needs much attention among all the criteria, followed by ecological, economic, information technology and dynamic capability theory. Further, change management, third-party audits and novel business models are key sub-factors to improve performance of sustainability in Industry 4.0 supply chains.

Practical implications
This study prioritized the performance factors of Industry 4.0 and sustainable supply chain in Indian manufacturing sector. These prioritized factors help to improve performance of organizations, which are practicing the Industry 4.0 and sustainability practices. Managers in manufacturing industries can use the SWARA for assessment of weights for the criteria and sub-criteria factors to take appropriate decisions to improve the organizations’ performance.

Originality/value
Managers in manufacturing industry can use these prioritized factors to improve the performance of their supply chains.
CIO equity compensation and IT investment: the moderating role of board monitoring and evidence of managerial myopia
Authors: Turedi Serdar , Erkan-Barlow Asligul 
Published: August 2022

PurposeThe purpose of this paper is to examine the effects of managerial myopia on information technology (IT) investment. Specifically, it aims to investigate the influence of chief information officer (CIO) compensation on IT investment and the moderating role of the board monitoring strength on this relationship.Design/methodology/approachThe study examines a sample of 194 firms listed on US stock exchanges with a CIO position in 2019. The authors employ hierarchical regression analysis to test the hypothesis.FindingsThe results show that CIO compensation negatively influences IT investment. Further, even though vigilant board monitoring does not necessarily reduce such opportunistic behaviors, weak board monitoring creates an environment for such actions.Research limitations/implicationsFirst, the cross-sectional data can limit the results' generalizability. Second, the sampling frame is not perfectly random as it consists of firms that have CIO compensation information in the ExecuComp for 2019. Third, we include only two measures of board monitoring strength.Practical implicationsBoard of directors should wisely select compensation packages' components since equity incentives potentially exacerbate managerial myopia. Moreover, firms may regulate CIOs' investment behaviors through board-level IT governance.Originality/valueThis study is one of the few studies that utilize CIO sensitivity to measure CIO compensation. Moreover, by examining the factors affecting IT investment behavior, this study sheds light on CIO incentives' impact on IT investment behaviors. Finally, to the best of the authors' knowledge, this is the first study to investigate board monitoring's role in the relationship between CIO sensitivity and IT investment intensity.
Inhibition of PSD95‐nNOS protein–protein interactions decreases morphine reward and relapse vulnerability in rats
Authors: Oliva Idaira , Saberi Shahin A. , Rangel‐Barajas Claudia , Iyer Vishakh , Bunner Kendra D. , Lai Yvonne Y. , Kulkarni Pushkar M. , Garai Sumanta , Thakur Ganesh A. , Crystal Jonathon D. , Rebec George V. , Hohmann Andrea G. 
Published: August 2022

AbstractGlutamate signalling through the N‐methyl‐d‐aspartate receptor (NMDAR) activates the enzyme neuronal nitric oxide synthase (nNOS) to produce the signalling molecule nitric oxide (NO). We hypothesized that disruption of the protein–protein interaction between nNOS and the scaffolding protein postsynaptic density 95 kDa (PSD95) would block NMDAR‐dependent NO signalling and represent a viable therapeutic route to decrease opioid reward and relapse‐like behaviour without the unwanted side effects of NMDAR antagonists. We used a conditioned place preference (CPP) paradigm to evaluate the impact of two small‐molecule PSD95‐nNOS inhibitors, IC87201 and ZL006, on the rewarding effects of morphine. Both IC87201 and ZL006 blocked morphine‐induced CPP at doses that lacked intrinsic rewarding or aversive properties. Furthermore, in vivo fast‐scan cyclic voltammetry (FSCV) was used to ascertain the impact of ZL006 on morphine‐induced increases in dopamine (DA) efflux in the nucleus accumbens shell (NAc shell) evoked by electrical stimulation of the medial forebrain bundle (MFB). ZL006 attenuated morphine‐induced increases in DA efflux at a dose that did not have intrinsic effects on DA transmission. We also employed multiple intravenous drug self‐administration approaches to examine the impact of ZL006 on the reinforcing effects of morphine. Interestingly, ZL006 did not alter acquisition or maintenance of morphine self‐administration, but reduced lever pressing in a morphine relapse test after forced abstinence. Our results provide behavioural and neurochemical support for the hypothesis that inhibition of PSD95‐nNOS protein–protein interactions decreases morphine reward and relapse‐like behaviour, highlighting a previously unreported application for these novel therapeutics in the treatment of opioid addiction.