bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cancer carrier screening in the general population using whole‐genome sequencing
Authors: Chang Ya‐Sian , Chao Dy‐San , Chung Chin‐Chun , Chou Yu‐Pao , Chang Chieh‐Min , Lin Chia‐Li , Chu Hou‐Wei , Chen Hon‐Da , Liu Ting‐Yuan , Juan Yu‐Hsuan , Chang Shun‐Jen , Chang Jan‐Gowth 
Published: July 2022

AbstractBackgroundCancer is a major cause of death, and its early identification and intervention have potential for clinical actionability and benefits for human health. The studies using whole‐genome sequencing (WGS) and large samples analysis of cancer‐related genes have been rarely done.MethodsWe performed WGS to explore germline mutations in coding and non‐coding areas of cancer‐related genes and non‐coding driver genes and regulatory areas. Structural variants (SVs) was also analyzed. We used several tools and a subgrouping method to analyze the variants in 1491 healthy participants. Moreover, 275 cancer‐related genes sequencing was carried out in 125 cancer patients.ResultsThe incidence of familial cancer in the Taiwanese general population is 8.79% (131/1491). Cancer carrier rate of cancer‐related genes is about 7.04% (105/1491) for pathogenic/likely pathogenic variants (P/LP) on ClinVar database only, and 28.24% (421/1491) for P/LP and loss of function variants. The carrier frequencies of cancer‐related genes P/LP on ClinVar database were as follows: 8.40% (11/131), 7.11% (28/394), and 6.83% (66/966) in FC, 1MC, and nMC, respectively. The SVs and non‐coding driver gene variants are uncommon. There are 1.54% (23/1491) of actionable cancer genes in American College of Medical Genetics and Genomics (ACMG), and the germline mutation rate of 275 cancer‐related genes is 7.2% (9/125) in cancer patients including 4.0% (5/125) of actionable cancer genes in ACMG. After analyzing the frequencies of P/LP variants on GJB2 and SLC25A13 genes, we suggest that these two genes may not be cancer‐related genes and need be re‐evaluated.ConclusionsWGS analysis can completely detect germline mutations in cancer carriers. This study use subgrouping approach for samples provides a strategy to study whether a gene or variant is a cancer‐related gene or variant in the future studies.
Pulsed electrolysis of carbon dioxide by large‐scale solid oxide electrolytic cells for intermittent renewable energy storage
Authors: Wu Anqi , Li Chaolei , Han Beibei , Liu Wu , Zhang Yang , Hanson Svenja , Guan Wanbing , Singhal Subhash C. 
Published: July 2022

AbstractCO2 electrolysis with solid oxide electrolytic cells (SOECs) using intermittently available renewable energy has potential applications for carbon neutrality and energy storage. In this study, a pulsed current strategy is used to replicate intermittent energy availability, and the stability and conversion rate of the cyclic operation by a large‐scale flat‐tube SOEC are studied. One hundred cycles under pulsed current ranging from −100 to −300 mA/cm2 with a total operating time of about 800 h were carried out. The results show that after 100 cycles, the cell voltage attenuates by 0.041%/cycle in the high current stage of −300 mA/cm2, indicating that the lifetime of the cell can reach up to about 500 cycles. The total CO2 conversion rate reached 52%, which is close to the theoretical value of 54.3% at −300 mA/cm2, and the calculated efficiency approached 98.2%, assuming heat recycling. This study illustrates the significant advantages of SOEC in efficient electrochemical energy conversion, carbon emission mitigation, and seasonal energy storage.
Palladium‐Catalyzed Intramolecular Dehydrogenative Arylboration of Alkenes
Authors: Zou Chenchen , Wu Hongli , Ji Yuqi , Zhang Penglin , Cui Huanhuan , Huang Genping , Zhang Chun 
Published: July 2022

Comprehensive SummaryThe palladium‐catalyzed borylative cyclization via C—H activation has been developed. By this chemistry, the indole‐fused dihydro‐pyrrole motif, which is a kind of important unit in natural products and bio‐active molecules, could be constructed and installed with a boric ester group. Furthermore, the utilities of products have been illustrated by the study of further transformations. Importantly, by using chiral ligand, the enantioselectivity of this borylative cyclization reaction could be controlled. Moreover, the borylative mechanism, which should proceed through a Pd(II)/Pd(IV) catalytic cycle, has been proposed based on the DFT calculations.
Acquired unilateral alopecia after arterial infusion chemotherapy in a recurrent nasopharyngeal carcinoma
Authors: Duan Bao‐Feng , Chen Hua‐Ying , Zheng Xue‐Mei , He Qing 
Published: July 2022

AbstractBackgroundIntractable nasopharyngeal hemorrhage is a severe complication with high mortality rate in patients with radiation therapy (RT) for nasopharyngeal carcinoma (NPC) that requires emergency treatment. Quite a few of them combine with tumor recurrence. Treatment planning for these patients is extremely difficult for oncologists, and effective treatments are lacking.CaseA 42‐year‐old man had a history of recurrent NPC that was treated with 2 cycles of chemoradiotherapies from 2017 to 2019. Five months after the second round of chemoradiotherapy, an episode of massive nasal bleeding occurred. As positron emission tomography (PET) scan revealed tumor recurrence in the left wall of nasopharynx, superselective embolization and subsequent intra‐arterial infusion (IA, 4 times of cisplatin 60 mg + fluorouracil 1.0 g) were performed to stop bleeding and achieve tumor control. To date, the disease‐free survival time has been over 1 year. No tumor recurrence or rebleeding is found except for alopecia on the left side.ConclusionsInterventional radiology is important and effective in the treatment of recurrent NPC for both massive nasal bleeding and tumor control. However, the unique complication of unilateral alopecia should not be ignored.
Development and validation of a forensic multiplex InDel assay: The AGCU InDel 60 kit
Authors: Liu Jing , Du Weian , Jiang Lirong , Liu Changhui , Chen Ling , Zheng Yangyang , Hou Yiping , Liu Chao , Wang Zheng 
Published: July 2022

AbstractMarker sets based on insertion/deletion polymorphisms (InDels) combine the characteristics of both short tandem repeats (STRs) and single nucleotide polymorphisms and have served as effective complementary or stand‐alone systems for human identification in forensics. We developed a novel multiplex amplification detection system, designated the AGCU InDel 60 kit, containing 57 autosomal InDels, 2 Y‐chromosomal InDels, and the amelogenin locus and validated the kit in a series of studies, which included tests of the PCR conditions; tests for sensitivity, species specificity, reproducibility, stability, and mock case samples; degradation studies; and a population study. The results indicated that the AGCU InDel 60 kit was accurate, specific, reproducible, stable, and robust. Complete DNA profiles were obtained even with 125 pg of human DNA. In tests of artificially degraded samples, we found that the number of alleles detected by the validated kit was considerably greater than that detected by the STR‐based AGCU 21+1 kit, even as the degree of degradation increased. Additionally, 564 unrelated individuals from three Han groups were investigated using this novel system, and the values of combined power of discrimination and combined power of exclusion were not less than 1–4.9026 × 10−24 and 1–3.1123 × 10−5, respectively. Thus, the results indicated that the novel kit was more powerful than the previous version of the InDel kit (the AGCU InDel 50 kit). Our results suggest that the AGCU InDel 60 kit can serve as an efficient tool for human forensics and a supplementary kit for population genetics research.
Differences in Trophic Level, Contaminant Load, and DNA Damage in an Urban and a Remote Herring Gull (Larus argentatus) Breeding Colony in Coastal Norway
Authors: Keilen Ellen Kristine , Borgå Katrine , Thorstensen Helene Skjeie , Hylland Ketil , Helberg Morten , Warner Nicholas , Bæk Kine , Reiertsen Tone Kristin , Ruus Anders 
Published: July 2022

Abstract
Herring gulls (Larus argentatus) are opportunistic feeders, resulting in contaminant exposure depending on area and habitat. We compared contaminant concentrations and dietary markers between two herring gull breeding colonies with different distances to extensive human activity and presumed contaminant exposure from the local marine diet. Furthermore, we investigated the integrity of DNA in white blood cells and sensitivity to oxidative stress. We analyzed blood from 15 herring gulls from each colony—the urban Oslofjord near the Norwegian capital Oslo in the temperate region and the remote Hornøya island in northern Norway, on the Barents Sea coast. Based on d13C and d34S, the dietary sources of urban gulls differed, with some individuals having a marine and others a more terrestrial dietary signal. All remote gulls had a marine dietary signal and higher relative trophic level than the urban marine feeding gulls. Concentrations (mean ± standard deviation [SD]) of most persistent organic pollutants, such as polychlorinated biphenyl ethers (PCBs) and perfluorooctane sulfonic acid (PFOS), were higher in urban marine (PCB153 17 ± 17 ng/g wet weight, PFOS 25 ± 21 ng/g wet wt) than urban terrestrial feeders (PCB153 3.7 ± 2.4 ng/g wet wt, PFOS 6.7 ± 10 ng/g wet wt). Despite feeding at a higher trophic level (d15N), the remote gulls (PCB153 17 ± 1221 ng/g wet wt, PFOS 19 ± 1421 ng/g wet wt) were similar to the urban marine feeders. Cyclic volatile methyl siloxanes were detected in only a few gulls, except for decamethylcyclopentasiloxane in the urban colony, which was found in 12 of 13 gulls. Only hexachlorobenzene was present in higher concentrations in the remote (2.6 ± 0.42 ng/g wet wt) compared with the urban colony (0.34 ± 0.33 ng/g wet wt). Baseline and induced DNA damage (doublestreak breaks) was higher in urban than in remote gulls for both terrestrial and marine feeders. Environ Toxicol Chem 2022;41:2466–2478. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Abstract
Generally higher pollution and DNA damage in urban breeding than remote breeding herring gulls, when adjusting to comparable trophic level. PCB = polychlorinated biphenyl ether; PBDE = polybrominated diphenyl ether; PFOS = perfluorooctane sulfonic acid; HCB = hexachlorobenzene.
Underwater sound speed profile parameters estimation in asynchronous network
Authors: Zandi Rahman , Dehghani Mohammad Javad , Kavoosi Vali 
Published: July 2022

AbstractThe technology of underwater wireless acoustic sensor networks (UWSNs) plays an important role in many commercial and military applications in underwater. In UWSNs, there exist many challenges posed by the specific characteristics of underwater acoustic channel, such as sound propagation speed is a function of depth with unknown parameters. Besides, time synchronization of anchor nodes is a crucial part of the applicable network. Time synchronization of anchor nodes as well as estimation of sound speed parameters considering an isogradient profile is explored here. While the previous efforts do not take into account these factors, this article assumes only one synchronized anchor node in the network and enables joint synchronization of the other anchor nodes and the estimation of the propagation delays between the anchor nodes using a weighted linear least square solution. Then, it uses the estimated propagation delays to estimate the unknown parameters of the sound speed profile using a conventional Gauss‐Newton algorithm with approximately three iterations to converge. Validation of the proposed method is done by the numerical simulations and to demonstrate the effectiveness of the proposed method we compare the results with Cramer‐Rao bound average.
Downregulating EVA1C exerts the potential to promote neuron growth after neonatal hypoxic‐ischemic encephalopathy injury associated with alternative splicing
Authors: Hu Yue , Rong Rong , Wang Yi , Yan Shan‐Shan , Liu Su , Wang Lei 
Published: July 2022

AbstractNeonatal hypoxic‐ischemic encephalopathy (NHIE) is one of the major diseases in newborns during the perinatal stage, which globally is the main reason for children's morbidity and mortality. However, the mechanism of NHIE still remains poorly clear. In this study, the 7‐day‐old rats were subjected to hypoxic‐ischemia (HI), then brain damage was detected. Afterward, the expression of eva‐1 homolog C (EVA1C) was measured in vitro by establishing the oxygen‐glucose deprivation (OGD) model in SHSY5Y cells and human fetal neurons. Subsequently, the potential function and mechanism of EVA1C were explored by silencing EVA1C and alternative splicing prediction. As a result, obvious neurobehavioral impairment and brain infarction were detected through Zea‐Longa score and TTC staining; meanwhile, neuron injury was tested by HE and Nissl staining post HI. Moreover, it was found that the expression of EVA1C was notably upregulated in SHSY5Y cells and human fetal neurons after OGD. In addition, cell survival and growth were increased after silencing EVA1C, which might be associated with alternative splicing. In conclusion, EVA1C interference exhibited potential in promoting neuron survival and growth, associated with exon skipping with the alternative splicing site in 34613318:34687258, which may provide the basis for the therapeutic target and mechanism research of NHIE.
Are we all on the same page? Subfield differences in open science practices in psychology
Authors: Rochios Christina , Richmond Jenny L. 
Published: July 2022

AbstractAlthough open science has become a popular tool to combat the replication crisis, it is unclear whether the uptake of open science practices has been consistent across the field of psychology. In this study, we were particularly interested in whether claims that developmental psychology lags behind other subfields in adopting open science practices were valid. To test this, we determined whether data and material sharing differed as a function of psychological subfield at the distinguished journal, Psychological Science. The results showed that open data and open materials scores increased from 2014–2015 to 2019–2020. Of note, articles published in the field of developmental psychology generated lower open data and open materials scores than articles published in cognition; however, scores were similar to articles published in social psychology. Across Psychological Science articles, shared data and materials were seldom accompanied by documentation that is likely to make shared research objects useful. These findings are discussed in the context of the unique challenges faces by developmental psychologists and how journals can more effectively encourage authors to practice open science across psychology.
Inhibition of endoplasmic reticulum stress and the downstream pathways protects CD4+ T cells against apoptosis and immune dysregulation in sepsis
Authors: Lu Gang , Li Qingqing , Liu Jianjun , Jia Yanan , Tang Jianguo , Zhang Xuemin 
Published: July 2022

AbstractImmunosuppression mediated by CD4+ T cell apoptosis and dysfunction is a key factor in promoting the progression of sepsis. Endoplasmic reticulum (ER) stress participates in the apoptosis and dysfunction of immune cells. We aimed to investigate the role of ER stress inhibition in CD4+ T cells in both in vitro and in vivo models of sepsis. In vitro model of sepsis was established with lipopolysaccharide (LPS) and the rat model of sepsis was established using cecal ligation and puncture (CLP). After the LPS treatment or CLP, ER stress inhibitors including 4‐PBA, SNJ‐1945, and SP600125 were used to treat cells or rats, and the CD4+ T cells were obtained by magnetic bead sorting. The effects of ER stress inhibitors on apoptosis and the function of CD4+ T cells were evaluated. After the LPS stimulation or CLP, the levels of ER stress and downstream markers (PERK, eIF2α, IRE‐1α, ATF6, ATF4, XBP‐1 s, GRP78, CHOP, and p‐JNK) were increased in CD4+ T cells at the beginning of sepsis. Meanwhile, the number of apoptotic CD4+ T cells markedly increased. In addition, sepsis impaired the function of CD4+ T cells, manifested by the increased population of Th1, Th2, Th17, and Treg, as well as the production of TNF‐α, interleukin (IL)‐6, IL‐4, and IL‐10. However, inhibitors of ER stress, JNK, and calpain all decreased the induction of Th1 and Th17, enhanced the increase of Th2 and Treg, decreased the production of TNF‐α and IL‐6, and enhanced the production of IL‐4 and IL‐10. Our findings indicate that ER stress inhibitors may play a protective role by reducing CD4+ T cell apoptosis and maintaining CD4+ T cell function, which may be useful for enhancing the immune function and poor prognosis of patients with sepsis.
Reevaluating the use of volatility factor in crop insurance premium rating
Authors: Bulut Harun , Fortenbery T. Randall 
Published: July 2022

AbstractThis article reevaluates the use of volatility factors in federal crop insurance rate setting. The analysis focuses on price risk for corn and soybeans over the period 1990–2021. The sensitivity of insurance premiums to changes in volatility is comparable to the sensitivity resulting from changes in crop prices. Risk Management Agency's (RMA) volatility factor is generally supported to be an unbiased and efficient estimator of the realized volatility, although this support is found to be marginal in the case of soybeans. The out‐of‐sample forecasting analysis indicates that RMA's current approach can be modified to better reflect price risk over the insurance period.
Implementation and evaluation of a standardized CMM practice management assessment tool in the Veterans Health Administration
Authors: McFarland Michael Shawn , Boggie Daniel , Ourth Heather , Groppi Julie , Moore Tera , Stephens Carolyn , Morreale Anthony 
Published: July 2022

AbstractComprehensive medication management (CMM) is defined as the standard of care that ensures each patient's medications are individually assessed to determine that each medication is appropriate for the patient, effective for the medical condition, safe given any comorbidities and other medications being taken, and able to be taken by the patient as intended. The practice management component of CMM can be defined as “the structural and system level supports within a practice related to practice management and operations that enable the efficiency, effectiveness, and sustainability of CMM services.” To date, there has been no consistent process in the Veterans Health Administration (VHA) to evaluate CMM practice management standardization. This article will describe the VHA effort in development, implementation, and outcomes of a CMM practice optimization tool modified from an existing tool. A modified version of practice management assessment tool was developed to integrate and align with VHA clinical pharmacy practice and policy. The VHA CMM Practice Optimization and Evaluation Tool (POET) was developed to access CMM practice management in multiple practice areas and was deployed for assessment in all VHA facilities. From May 2021 to March 2022, 352 practice area evaluations were completed at 152 VHA facilities using POET. Total submission practice area detail was gathered from 245 Patient Aligned Care Team (primary care), 32 outpatient mental health, 29 acute care, 11 pain, 11 specialty ambulatory care, 8 geriatric community living centers (eg, long‐term care) or hospice, 6 home‐based primary care, 4 critical care, 4 emergency department, and 2 residential rehabilitation and treatment programs. This work evaluating the five essential core practice management domains of CMM using a standardized evaluation process revealed that the VHA could adapt a tool that yields immeasurable information for standardization of CMM provided by a clinical pharmacist.
Uncovering the hidden reactivity of benzyne/aryne precursors utilized under milder condition: Bonding and stability studies by EDA‐NOCV analyses
Authors: Gorantla Sai Manoj N. V. T. , Mondal Kartik Chandra 
Published: July 2022

AbstractArenes [C6H3R(TMS)(OTf); also called benzyne/aryne precursors] containing inter‐related leaving groups Me3Si (TMS) and CF3SO3(OTf) on the adjacent positions (1,2‐position) are generally converted to their corresponding aryne‐intermediates via the addition of fluoride anion (F−) and subsequent elimination of TMS and OTf groups. This reaction is believed to proceed via the formation of an anionic intermediate [C6H4(TMS‐F)(OTf)]−. The EDA‐NOCV analysis (EDA‐NOCV = energy decomposition analysis‐natural orbital for chemical valence) of over 35 such precursors of varied types have been reported to reveal bonding and stability of CArSi and COTf bonds. EDA‐NOCV showed that the nature of the CArSi bond of C6H3R(TMS)(OTf) can be expressed as both dative and electron sharing [CArSi, CAr→Si]. The CArOTf bond, on the other hand, can be described explicitly as dative [CAr←OTf]. The nature of CArSi bond of [C6H4(TMS‐F)(OTf)]− exclusively changes to covalent dative σ‐bond CAr→S(Me)3F on the attachment of F− to the TMS group of C6H4(TMS)(OTf). Introduction of σ‐electron withdrawing group (like OMe, NMe2, and NO2) to the ortho‐position of the TMS group of functionalized arynes C6H3R(TMS)(OTf) prefer to have a covalent dative σ‐bond (CAr→Si) over an electron‐sharing covalent σ‐bond (CArSi). If this σ‐electron withdrawing group is shifted from ortho‐position to meta‐ and para‐positions, then the preference for a dative bond decreases significantly, implying that the electronic effect on the nature of chemical bonds affects through bond paths. This effect dies with distance, similar to the well‐known inductive effect.
Sensitivity of seasonal air temperature and precipitation, and onset of snowmelt, to Arctic Dipole modes across the Taiga Plains, Northwest Territories, Canada
Authors: Persaud Bhaleka D. , Chasmer Laura E. , Quinton William L. , Wolfe Brent B. , English Michael C. 
Published: July 2022

AbstractNorthern high latitudes are experiencing some of the greatest increases in air temperatures on Earth. Air temperatures (along with other modulating variables including precipitation and the onset of snowmelt) are influenced by atmospheric–oceanic circulation patterns, some of which are persistent and recurrent. One pattern in particular, the Arctic Dipole (AD) anomaly, is a persistent sea‐level pressure teleconnection pattern between the Canadian Archipelago and Barents Sea that has unknown impacts on local climate variability. These patterns may be important, especially in hydro‐ecologically sensitive areas such as Northwest Territories (NWT), Canada where permafrost thaw and ecosystem changes are influenced by interannual climate variability. The goal of this research is to determine the impacts of the AD on local climate (air temperature, precipitation, snowmelt) for a 66‐year period (1950–2015) spanning both latitudinal and longitudinal gradients across NWT from north to south and foothills to plains. Deviations during strong positive and negative modes of the AD index were calculated in reference to the complete 66‐year record. Results showed considerable year‐to‐year variability in the AD pattern, with more frequent strong negative modes during the 2000s. During 1950–2015, there were 64 and 56 occurrences of strong positive and strong negative AD modes, respectively, across all seasons. Spring and summer strong AD modes led to local air temperature anomalies of greater than 0.8°C compared with the long‐term (66 years) mean. Earlier onset of snowmelt, by an average of 3–5 days, was also noted during positive AD modes. Despite strong connectivity between the AD and local air temperature, we found less correspondence between the AD and seasonal precipitation. These findings improve understanding of the impacts of the AD on local weather and climate in NWT and suggest implications for ecosystem change, such as drying and shrubification of northern boreal peatlands and possible connectivity to teleconnection impacts on wildland fire.