bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recognizing the equity implications of restoration priority maps
Authors: Schultz Bill , Brockington Dan , Coleman Eric A , Djenontin Ida , Fischer Harry W , Fleischman Forrest , Kashwan Prakash , Marquardt Kristina , Pfeifer Marion , Pritchard Rose , Ramprasad Vijay 
Published: October 2022

Abstract
A growing number of studies seek to identify global priority areas for conservation and restoration. These studies often produce maps that highlight the benefits of concentrating such activity in the tropics. However, the potential equity implications of using these prioritization exercises to guide global policy are less often explored and articulated. We highlight those equity issues by examining a widely publicized restoration priority map as an illustrative case. This map is based on a prioritization analysis that sought to identify places where restoration of agricultural land might provide the greatest biodiversity and carbon sequestration benefits at the lowest cost. First, we calculate the proportion of agricultural land in countries around the world that the map classifies as a top 15% restoration priority. A regression analysis shows that this map prioritizes restoration in countries where displacing agriculture may be most detrimental to livelihoods: countries that are poorer, more populated, more economically unequal, less food secure, and that employ more people in agriculture. Second, we show through another regression analysis that a similar pattern appears sub-nationally within the tropics: 5 km × 5 km parcels of land in the tropics that are less economically developed or more populated are more likely to be top 15% restoration priorities. In other words, equity concerns persist at a subnational scale even after putting aside comparisons between the tropics and the Global North. Restorative activity may be beneficial or harmful to local livelihoods depending on its conceptualization, implementation, and management. Our findings underline a need for prioritization exercises to better attend to the risks of concentrating potentially negative livelihood impacts in vulnerable regions. We join other scholars calling for greater integration of social data into restoration science.
All-cause and in-hospital mortality after aspirin use in patients hospitalized with COVID-19: a systematic review and meta-analysis
Authors: Baral Nischit , Mitchell Joshua D , Savarapu Pramod K , Akanbi Maxwell , Acharya Bandana , Kambalapalli Soumya , Seri Amith , Bashyal Krishna P , Kunadi Arvind , Ojha Niranjan , Volgman Annabelle Santos , Gupta Tripti , Paul Timir K 
Published: October 2022

AbstractBackgroundWith the results of the largest randomized controlled trial (RECOVERY) and the most extensive retrospective cohort study on coronavirus disease 2019 (COVID-19) recently published, we performed a meta-analysis on the association of aspirin with mortality of COVID-19. We aimed to investigate the role of aspirin in COVID-19 hospitalizations.Materials and MethodsWe searched PubMed, EMBASE and Cochrane databases for studies from 1 January 2020 until 20 July 2022, that compared aspirin versus non-aspirin use in hospitalized COVID-19 patients. We excluded case reports, review articles and studies on non-hospitalized COVID-19 infections. We used the inverse variance method and random effects model to pool the individual studies.ResultsTen observational studies and one randomized controlled trial met the criteria for inclusion. There were 136 695 total patients, of which 27 168 were in the aspirin group and 109 527 were in the non-aspirin group. Aspirin use was associated with a 14% decrease in all-cause mortality compared with non-aspirin use in patients hospitalized with COVID-19 [relative risk (RR) 0.86, confidence interval (95% CI) 0.76–0.97; P = 0.002; I2 =64%]. Among subgroups of studies reporting in-hospital mortality in COVID-19 hospitalizations, aspirin use was associated with a 16% decrease in in-hospital mortality compared with non-aspirin use (RR 0.84, 95% CI 0.71–0.99; P = 0.007; I2 =64%).ConclusionOur study shows that aspirin decreases in-hospital mortality in patients hospitalized with COVID-19. Further studies are needed to assess which COVID-19 patient populations benefit most, such as patients on aspirin for primary versus secondary prevention of atherosclerotic disease. In addition, significant bleeding also needs to be considered when assessing the risk–benefit of aspirin use.
Common signatures of differential microRNA expression in Parkinson’s and Alzheimer’s disease brains
Authors: Dobricic Valerija , Schilling Marcel , Farkas Ildiko , Gveric Djordje O , Ohlei Olena , Schulz Jessica , Middleton Lefkos , Gentleman Steve M , Parkkinen Laura , Bertram Lars , Lill Christina M 
Published: October 2022

AbstractDysregulation of microRNA gene expression has been implicated in many neurodegenerative diseases, including Parkinson’s disease. However, the individual dysregulated microRNAs remain largely unknown. Previous meta-analyses have highlighted several microRNAs being differentially expressed in post-mortem Parkinson’s disease and Alzheimer's disease brains versus controls, but they were based on small sample sizes. In this study, we quantified the expression of the most compelling Parkinson’s and Alzheimer’s disease microRNAs from these meta-analyses (‘candidate miRNAs’) in one of the largest Parkinson’s/Alzheimer’s disease case–control post-mortem brain collections available (n = 451), thereby quadruplicating previously investigated sample sizes. Parkinson’s disease candidate microRNA hsa-miR-132-3p was differentially expressed in our Parkinson’s (P = 4.89E−06) and Alzheimer’s disease samples (P = 3.20E−24) compared with controls. Alzheimer’s disease candidate microRNAs hsa-miR-132-5p (P = 4.52E−06) and hsa-miR-129-5p (P = 0.0379) were differentially expressed in our Parkinson’s disease samples. Combining these novel data with previously published data substantially improved the statistical support (α = 3.85E−03) of the corresponding meta-analyses, clearly implicating these microRNAs in both Parkinson’s and Alzheimer’s disease. Furthermore, hsa-miR-132-3p/-5p (but not hsa-miR-129-5p) showed association with α-synuclein neuropathological Braak staging (P = 3.51E−03/P = 0.0117), suggesting that hsa-miR-132-3p/-5p play a role in α-synuclein aggregation beyond the early disease phase. Our study represents the largest independent assessment of recently highlighted candidate microRNAs in Parkinson’s and Alzheimer’s disease brains, to date. Our results implicate hsa-miR-132-3p/-5p and hsa-miR-129-5p to be differentially expressed in both Parkinson’s and Alzheimer’s disease, pinpointing shared pathogenic mechanisms across these neurodegenerative diseases. Intriguingly, based on publicly available high-throughput sequencing of RNA isolated by cross-linking immunoprecipitation data, hsa-miR-132 may interact with SNCA messenger RNA in the human brain, possibly pinpointing novel therapeutic approaches in fighting Parkinson’s disease.
A Lattice-Based Redactable Signature Scheme using Cryptographic Accumulators for Trees
Authors: Zhao Yong , Yang Shaojun , Wu Wei , Huang Xinyi 
Published: October 2022

Abstract
Redactable signatures allow the signature holder to remove admissible data blocks in the signed data while generating valid signatures about different redacted data without communicating with the primary signer. Now, this sort of signature has attracted widespread attention due to its many application scenarios such as electronic medical records, smart grids and XML files. However, there are rarely redactable signature schemes that can resist quantum attacks so far. In the wake of quantum calculation era, it is essential to blossom more quantum-resistant redactable signatures for different data structures. Moreover, it is popular to use accumulators to design redactable signature schemes. Unfortunately, the existing accumulators do not support tree data structures. Therefore, this paper first gives the definition of accumulator schemes for trees, and designs a lattice-based accumulator scheme for trees. Our accumulator scheme features shorter accumulator values and a faster witness generation algorithm than existing lattice trapdoor accumulators. Second, this paper resorts to approximate trapdoors and the preimage sampleable technique, and presents a lattice-based redactable signature scheme for trees using our accumulator scheme. Meanwhile, this scheme fulfills unforgeability, transparency and privacy under adaptive chosen-message attacks. Furthermore, the experiment results show that the redactable signature scheme meets actual revision requirements well.
The mating system of Himalayan marmots as inferred by microsatellite markers
Authors: Chen Yi , Guo Cheng , Zhou Shuailing , Xiang Zuofu 
Published: October 2022

Abstract
The Himalayan marmot Marmota himalayana is widely distributed across the Qinghai–Tibetan Plateau and lives in social groups, yet the mating system of this highly social marmot species is unknown. In this study, the genetic mating system of Himalayan marmots was investigated using microsatellite markers to determine which mating strategies individuals employ. Results revealed that both monogamous and polygamous mating relationships occur in our study population, indicating that the genetic mating system of this marmot species is promiscuity. This study presents the first genetic evidence on the mating system for Himalayan marmots, yet indicates that further studies employing both a genetic and behavioral framework are needed to better understand the social structure and reproductive biology of this marmot species.
Same place, different time, head up: Multiple antipredator responses to a recolonizing apex predator
Authors: Esattore Bruno , Rossi Agnese Carlotta , Bazzoni Francesco , Riggio Chiara , Oliveira Raquel , Leggiero Ivan , Ferretti Francesco 
Published: October 2022

Abstract
Prey adjust their antipredator behavioral tactics to minimize the risk of an encounter with predators. Spatiotemporal responses of prey to predators have been reported, but the nature of antipredator response is not ubiquitous and it is the object of increasing interest, especially considering the recent recovery of large carnivores in Europe, and the potential for behavioral antipredator responses to elicit consequences at the ecosystem level. We have tested multiple antipredator responses by fallow deer Dama dama to wolf Canis lupus in a Mediterranean protected area recently recolonized by this apex predator. Through intensive camera trapping, we tested for temporal and spatial association between predator and prey, and we have also studied deer vigilance in forest habitats where focal observations are usually impossible. Wolf detection rates were spatially associated with those of fallow deer. Accordingly, no evidence was found for fallow deer avoiding sites with higher predator detection rates. Temporal activity patterns were significantly different between the 2 species, with the wolf being mainly nocturnal whereas fallow deer was active especially during daylight. A comparison with a preliminary study strongly suggests an increase in the diurnal activity of fallow deer along with the stabilization of wolf presence in the area. Both the rate and the duration of vigilance of female fallow deer increased with the local frequency of wolf activity. We suggest an antipredator response based on temporal—rather than spatial—avoidance, as well as increased vigilance.
Bleeding and ischaemic events after first bleed in anticoagulated atrial fibrillation patients: risk and timing
Authors: Meyre Pascal B , Blum Steffen , Hennings Elisa , Aeschbacher Stefanie , Reichlin Tobias , Rodondi Nicolas , Beer Jürg H , Stauber Annina , Müller Andreas , Sinnecker Tim , Moutzouri Elisavet , Paladini Rebecca E , Moschovitis Giorgio , Conte Giulio , Auricchio Angelo , Ramadani Alexandra , Schwenkglenks Matthias , Bonati Leo H , Kühne Michael , Osswald Stefan , Conen David 
Published: October 2022

Abstract

Aims
To determine the risk of subsequent adverse clinical outcomes in anticoagulated patients with atrial fibrillation (AF) who experienced a new bleeding event.

Methods and results
Anticoagulated AF patients were followed in two prospective cohort studies. Information on incident bleeding was systematically collected during yearly follow-up visits and events were adjudicated as major bleeding or clinically relevant non-major bleeding (CRNMB) according to the International Society on Thrombosis and Haemostasis guidelines. The primary outcome was a composite of stroke, myocardial infarction (MI), or all-cause death. Time-updated multivariable Cox proportional-hazards models were used to compare outcomes in patients with and without incident bleeding. Median follow-up was 4.08 years [interquartile range (IQR): 2.93–5.98]. Of the 3277 patients included (mean age 72 years, 28.5% women), 646 (19.7%) developed a new bleeding, 297 (9.1%) a major bleeding and 418 (12.8%) a CRNMB. The incidence of the primary outcome was 7.08 and 4.04 per 100 patient-years in patients with and without any bleeding [adjusted hazard ratio (aHR): 1.36, 95% confidence interval (CI): 1.16–1.61; P &lt; 0.001; median time between a new bleeding and a primary outcome 306 days (IQR: 23–832)]. Recurrent bleeding occurred in 126 patients [incidence, 8.65 per 100 patient-years (95% CI: 7.26–10.30)]. In patients with and without a major bleeding, the incidence of the primary outcome was 11.00 and 4.06 per 100 patient-years [aHR: 2.04, 95% CI: 1.69–2.46; P &lt; 0.001; median time to a primary outcome 142 days (IQR: 9–518)], and 59 had recurrent bleeding [11.61 per 100 patient-years (95% CI: 8.99–14.98)]. The incidence of the primary outcome was 5.29 and 4.55 in patients with and without CRNMB [aHR: 0.94, 95% CI: 0.76–1.15; P = 0.53; median time to a composite outcome 505 days (IQR: 153–1079)], and 87 had recurrent bleeding [8.43 per 100 patient-years (95% CI: 6.83–10.40)]. Patients who had their oral anticoagulation (OAC) discontinued after their first bleeding episode had a higher incidence of the primary composite than those who continued OAC (63/89 vs. 159/557 patients; aHR: 4.46, 95% CI: 3.16–6.31; P &lt; 0.001).

Conclusion
In anticoagulated AF patients, major bleeding but not CRNMB was associated with a high risk of adverse outcomes, part of which may be explained by OAC discontinuation. Most events occurred late after the bleeding episode, emphasizing the importance of long-term follow-up in these patients.
Standardized pre-procedural clinical workup for protected percutaneous coronary intervention
Authors: Al-Rashid Fadi , Van Mieghem Nicolas M , Bonello Laurent , Oreglia Jacopo , Romagnoli Enrico 
Published: October 2022

Abstract
In addition to appropriate patient screening, pre-procedural preparation is essential to optimize both technical success and patient outcome for protected percutaneous coronary intervention (PCI). A critical component of optimization is the identification and preparation of a suitable femoral access site. Here, we describe several options for both imaging and image-guided access to optimize the approach.
Integrative analysis of transcriptome reveals the possible mechanism of delayed leaf senescence in pak choi (Brassica rapa subsp. chinensis) following melatonin treatment
Authors: Liu Xuesong , Li Yuntong , Zhu Junzhen , Li Pengxia 
Published: October 2022

Abstract
Melatonin (MT) is a low molecular weight compound with a wide variety of biological functions in plants. It is known to delay senescence in various fruits and vegetables. This study demonstrates that MT (100 μmol/L) delayed the senescence of pak choi stored at 20 °C. A total of 1121 differentially expressed genes (DEGs) were identified in an MT-treated group in comparison to untreated pak choi. Analysis of DEGs revealed that MT-induced delay in senescence involved reduction in the expression of chlorophyll metabolism genes and regulation of the expression of hormone-related genes and leaf senescence-related transcription factors. MT decreased the activity of chlorophyll metabolism-related enzymes Mg-dechelatase, pheophytinase, and pheide a oxygenase, decreased the concentrations of abscisic acid and jasmonic acid, and increased auxin concentration. MT delayed the impact of senescence-related transcription factors BcNAC41 and BcNAC87 on tobacco leaf senescence. This study provides an insight into the molecular mechanisms of MT-induced delay of postharvest senescence in pak choi and confirms its potential as a preservation technique.
Gene Recruitments and Dismissals in the Argonaut Genome Provide Insights into Pelagic Lifestyle Adaptation and Shell-like Eggcase Reacquisition
Authors: Yoshida Masa-aki , Hirota Kazuki , Imoto Junichi , Okuno Miki , Tanaka Hiroyuki , Kajitani Rei , Toyoda Atsushi , Itoh Takehiko , Ikeo Kazuho , Sasaki Takenori , Setiamarga Davin H E 
Published: October 2022

Abstract
The paper nautilus or greater argonaut, Argonauta argo, is a species of octopods which is characterized by its pelagic lifestyle and by the presence of a protective spiral-shaped shell-like eggcase in females. To reveal the genomic background of how the species adapted to the pelagic lifestyle and acquired its shell-like eggcase, we sequenced the draft genome of the species. The genome size was 1.1 Gb, which is the smallest among the cephalopods known to date, with the top 215 scaffolds (average length 5,064,479 bp) covering 81% (1.09 Gb) of the total assembly. A total of 26,433 protein-coding genes were predicted from 16,802 assembled scaffolds. From these, we identified nearly intact HOX, Parahox, Wnt clusters, and some gene clusters that could probably be related to the pelagic lifestyle, such as reflectin, tyrosinase, and opsin. The gene models also revealed several homologous genes related to calcified shell formation in Conchiferan mollusks, such as Pif-like, SOD, and TRX. Interestingly, comparative genomics analysis revealed that the homologous genes for such genes were also found in the genome of the shell-less octopus, as well as Nautilus, which has a true outer shell. Therefore, the draft genome sequence of Arg. argo presented here has helped us to gain further insights into the genetic background of the dynamic recruitment and dismissal of genes to form an important, converging extended phenotypic structure such as the shell and the shell-like eggcase. Additionally, it allows us to explore the evolution of from benthic to pelagic lifestyles in cephalopods and octopods.
A salutogenic urban design framework: the case of UK local high streets and older people
Authors: Brunelli Luca , Smith Harry , Woolrych Ryan 
Published: October 2022

Summary
The article provides a novel look at the links between salutogenesis, health promotion, and urban design supported by the findings of recent research on local high streets and their benefits for the well-being of older people. Salutogenesis and the related explanatory concept of sense of coherence (SOC) have provided a theoretical framework for developing healthy settings interventions, shifting the focus from exploring barriers and deficits to assets and resources in promoting people’s health and well-being. While these concepts have informed policies and programmes at the level of regions and cities, no attempt has been made to establish more direct links with the disciplines devoted to the organization and design of the built environment at the scale of public spaces and streets. This article advances the idea that the main categories of SOC—comprehensibility, manageability and meaningfulness—have found application in urban design theory. Linking these categories with urban design concepts in a comprehensive framework, it is possible to guide interventions aimed at strenghtening well-being resources available in the public realm. This is corroborated by the findings resulting from a study of the well-being experiences of older people (n = 84) across a range of local high streets in the city of Edinburgh (UK) applying an innovative multi-methods approach. The discussion establishes the links between well-being benefits, SOC constructs and urban design concepts, and underscores the potential of the proposed framework to guide a design-oriented salutogenic approach to the built environment.
Cucumber glossy fruit 1 (CsGLF1) encodes the zinc finger protein 6 that regulates fruit glossiness by enhancing cuticular wax biosynthesis
Authors: Yang Yan , Cai Congxi , Wang Yipeng , Wang Yanran , Ju Haolun , Chen Xuehao 
Published: October 2022

Abstract
Cucumber glossiness is an important visual quality trait that affects consumer choice. Accumulating evidence suggests that glossy trait is associated with cuticular wax accumulation. However, the molecular genetic mechanism controlling cucumber glossiness remains largely unknown. Here, we report the map-based cloning and functional characterization of CsGLF1, a locus that determines the glossy trait in cucumber. CsGLF1 encodes a homolog of the Cys2His2-like fold group (C2H2) -type zinc finger protein 6 (ZFP6) and its deletion leads to glossier pericarp and decreased cuticular wax accumulation. Consistently, transcriptomic analysis demonstrated that a group of wax biosynthetic genes were downregulated when CsZFP6 was absent. Further, transient expression assay revealed that CsZFP6 acted as a transcription activator of cuticular wax biosynthetic genes. Taken together, our findings demonstrated a novel regulator of fruit glossiness, which will provide new insights into regulatory mechanism of fruit glossiness in cucumber.
Gapless genome assembly of azalea and multi-omics investigation into divergence between two species with distinct flower color
Authors: Nie Shuai , Zhao Shi-Wei , Shi Tian-Le , Zhao Wei , Zhang Ren-Gang , Tian Xue-Chan , Guo Jing-Fang , Yan Xue-Mei , Bao Yu-Tao , Li Zhi-Chao , Kong Lei , Ma Hai-Yao , Chen Zhao-Yang , Liu Hui , El-Kassaby Yousry A , Porth Ilga , Yang Fu-Sheng , Mao Jian-Feng 
Published: October 2022

Abstract
The genus Rhododendron (Ericaceae), with more than 1000 species highly diverse in flower color, is providing distinct ornamental values and a model system for flower color studies. Here, we investigated the divergence between two parental species with different flower color widely used for azalea breeding. Gapless genome assembly was generated for the yellow-flowered azalea, Rhododendron molle. Comparative genomics found recent proliferation of long terminal repeat retrotransposons (LTR-RTs), especially Gypsy, has resulted in a 125 Mb (19%) genome size increase in species-specific regions, and a significant amount of dispersed gene duplicates (13 402) and pseudogenes (17 437). Metabolomic assessment revealed that yellow flower coloration is attributed to the dynamic changes of carotenoids/flavonols biosynthesis and chlorophyll degradation. Time-ordered gene co-expression networks (TO-GCNs) and the comparison confirmed the metabolome and uncovered the specific gene regulatory changes underpinning the distinct flower pigmentation. B3 and ERF TFs were found dominating the gene regulation of carotenoids/flavonols characterized pigmentation in R. molle, while WRKY, ERF, WD40, C2H2, and NAC TFs collectively regulated the anthocyanins characterized pigmentation in the red-flowered R simsii. This study employed a multi-omics strategy in disentangling the complex divergence between two important azaleas and provided references for further functional genetics and molecular breeding.