bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aminoacylation‐defective bi‐allelic mutations in human EPRS1 associated with psychomotor developmental delay, epilepsy, and deafness
Authors: Jin Danni , Wek Sheree A. , Cordova Ricardo A. , Wek Ronald C. , Lacombe Didier , Michaud Vincent , Musier‐Forsyth Karin 
Published: November 2022

AbstractAminoacyl‐tRNA synthetases are enzymes that ensure accurate protein synthesis. Variants of the dual‐functional cytoplasmic human glutamyl‐prolyl‐tRNA synthetase, EPRS1, have been associated with leukodystrophy, diabetes and bone disease. Here, we report compound heterozygous variants in EPRS1 in a 4‐year‐old female patient presenting with psychomotor developmental delay, seizures and deafness. Functional studies of these two missense mutations support major defects in enzymatic function in vitro and contributed to confirmation of the diagnosis.
Marginal bone loss around dental implants: comparison between matched groups of bruxer and non‐bruxer patients: A retrospective case–control study
Authors: Bredberg Clara , Vu Camila , Häggman‐Henrikson Birgitta , Chrcanovic Bruno Ramos 
Published: November 2022

AbstractPurposeTo compare marginal bone loss (MBL) around dental implants in a group of bruxers in relation to a matched group of non‐bruxers.MethodsThe present record‐based retrospective study included patients selected from individuals treated with dental implants at one specialist clinic in Malmö. Only implants not lost and with baseline radiographs taken within 12 months after implant placement and with a minimum of 36 months of radiological follow‐up were considered for inclusion. Univariate linear regression models and a linear mixed‐effects model were performed.ResultsTwo hundred and four patients (104 bruxers, 100 non‐bruxers), with a total of 811 implants (416 in bruxers, 395 in non‐bruxers) were included in the study. The results of the linear mixed‐effects model suggested that bruxism, smoking, age, region of the jaws, implant diameter, and prosthesis type had a statistically significant influence on MBL over time. Individuals who are both bruxers and smokers showed greater MBL when compared to individuals who are either a bruxer or smoker, or neither (p < 0.001).ConclusionsBruxism is suggested to increase the risk of MBL over time, as well as higher age, smoking, and the combination of bruxism and smoking. Other factors that showed a correlation with increased MBL were implant diameter, region of the jaws, and prosthesis type, but it is not possible to draw robust conclusions for these factors, as the categories of these variables were very unbalanced.
Recurrence of diverticulitis after prophylactic sigmoidectomy: an underestimated problem?
Authors: Mathilde Aubert , Mege Diane , Monsinjon Marie , Giacca Massimo , Panis Yves 
Published: November 2022

AbstractAimSeveral papers have been published about the risk of recurrence after an attack of diverticulitis treated conservatively. However, very few papers have been devoted to the risk of postoperative recurrence of diverticulitis (PRD) after prophylactic sigmoidectomy (PS). The aim of this work was to report the rate of PRD after PS and to assess possible risk factors for recurrence after surgery.MethodAll consecutive patients who underwent elective laparoscopic PS for diverticulitis between 2005 and 2019 were retrospectively included. PRD was assessed.ResultsThree hundred and sixty four patients (199 men, mean age 54 ± 13 years) were included. Among these, 26 (7%) presented with 1.7 ± 1 (range 1–4) episodes of recurrence of diverticulitis after a mean delay of 44 ± 39 months (1 month–11 years) after surgery. Patients who presented with postoperative recurrence of diverticulitis were younger (46 ± 11 vs. 55 ± 13 years, p = 0.002) and more frequently had uncomplicated diverticulitis [15/26 (58%) vs. 97/338 (29%), p = 0.002] and more than two previous episodes before PS [17/26 (65%) vs. 132/338 (39%), p = 0.009] than patients without PRD. After multivariate analysis, two independent risk factors for PRD were identified: patients with more than two episodes before PS (OR = 3.3, 95% CI = 1.2–9, p = 0.005) and age < 50 years (OR = 4.5, 95% CI = 2–11, p = 0.001). If both factors were present, recurrence reached 18% (9/51).ConclusionPostoperative recurrence of diverticulitis is rare (7%) after PS for diverticulitis. Some patients (i.e. those with more than two episodes before PS and/or age <50 years) could be exposed to a higher risk of recurrence (up to 18%), making prophylactic surgery questionable in these patients.
Comparison of static and rolling logistic regression models on predicting invasive mechanical ventilation or death from COVID‐19—A retrospective, multicentre study
Authors: Engoren Milo , Pancaro Carlo , Yeldo Nicholas S. , Kerzabi Lotfi S. , Douville Nicholas 
Published: November 2022

AbstractIntroductionCOVID‐19 virus has undergone mutations, and the introduction of vaccines and effective treatments have changed its clinical severity. We hypothesized that models that evolve may better predict invasive mechanical ventilation or death than do static models.MethodsThis retrospective study of adult patients with COVID‐19 from six Michigan hospitals analysed 20 demographic, comorbid, vital sign and laboratory factors, one derived factor and nine factors representing changes in vital signs or laboratory values with time for their ability to predict death or invasive mechanical ventilation within the next 4, 8 or 24 h. Static logistic regression was constructed on the initial 300 patients and tested on the remaining 6741 patients. Rolling logistic regression was similarly constructed on the initial 300 patients, but then new patients were added, and older patients removed. Each new construction model was subsequently tested on the next patient. Static and rolling models were compared with receiver operator characteristic and precision‐recall curves.ResultsOf the 7041 patients, 534 (7.6%) required invasive mechanical ventilation or died within 14 days of arrival. Rolling models improved discrimination (0.865 ± 0.010, 0.856 ± 0.007 and 0.843 ± 0.005 for the 4, 8 and 24‐h models, respectively; all p < 0.001 compared with the static logistic regressions with 0.827 ± 0.011, 0.794 ± 0.012 and 0.735 ± 0.012, respectively). Similarly, the areas under the precision‐recall curves improved from 0.006, 0.010 and 0.021 with the static models to 0.030, 0.045 and 0.076 for the 4‐, 8‐ and 24‐h rolling models, respectively, all p < 0.001.ConclusionRolling models with contemporaneous data maintained better metrics of performance than static models, which used older data.
A randomized, open‐label, two‐treatment crossover study to evaluate the effect of food on the pharmacokinetics of diazepam nasal spray in healthy adults
Authors: Rogawski Michael A. , Slatko Gary 
Published: November 2022

AbstractObjectiveThe pharmacokinetics of oral diazepam are affected by food, but food‐effect studies have not been conducted for diazepam nasal spray because it is believed that most absorption occurs via the nasal mucosa. However, gastrointestinal side effects reported with nasal diazepam suggest that at least a portion of the drug may be absorbed enterally and thus subject to food effects. The objective of this study was to evaluate the possible effects of food on the pharmacokinetics of diazepam nasal spray in healthy adults.MethodsThis randomized, open‐label crossover study compared equal doses of diazepam nasal spray after an overnight fast and after a standardized high‐fat, high‐calorie breakfast. Each participant served as their own control, and there was a washout period of at least 21 days between treatments.ResultsTwenty‐four healthy adults enrolled in this study. Two participants withdrew consent, and two had pre‐dose diazepam concentrations that exceeded the protocol‐defined minimum after the washout period and were excluded from the final analysis population of 20 participants. Under fed conditions, the mean maximum plasma diazepam concentration was decreased by 48% (p < .0001) and the overall diazepam exposure during the first 4 h was reduced by 57% (p < .0001) compared with fasted conditions. The time to maximum plasma concentration was 4.0 h in the fed state compared with 2.0 h in the fasted state (p < .0001). At 2 h post‐dose, diazepam concentrations were ≥150 ng/mL for 100% of the participants when in the fasted state and 30% when in the fed state. Significantly more participants experienced adverse events under fasted conditions (83.3%) than under fed conditions (54.5%; p = .0340).SignificanceThis study in healthy volunteers demonstrated that food significantly decreases and delays the absorption of diazepam dosed via nasal spray. Patients using diazepam nasal spray after eating may obtain diazepam concentrations that are below those needed for seizure control.
Classification of open source software bug report based on transfer learning
Authors: Zhifang Liao , Kun Wang , Qi Zeng , Shengzong Liu , Yan Zhang , Jianbiao He 
Published: November 2022

AbstractCurrently, the feature richness of text encoding vectors in the bug report classification model based on deep learning is limited by the size of the domain dataset and the quality of the text. However, it is difficult to further enrich the features of text encoding vectors. At the same time, most existing bug report classification methods ignore the submitter's personal information. To solve these problems, we construct nine personal information characteristics of bug report submitters in GitHub by survey. Then, we propose a GitHub bug report classification method named personal information fine‐tuning network (PIFTNet) based on transfer learning and the submitter's personal information. PIFTNet transfers the general text feature vectors in bidirectional encoder representation from transformers (BERT) to the domain of bug report classification by fine‐tuning the pre‐training parameters in BERT. It also combines the text characteristics and the characteristics of the submitter's personal information to construct the classification model. In addition, we propose a two‐stage training method to alleviate the catastrophic changes in the pre‐training parameters and loss of the initially learned knowledge caused by direct training of PIFTNet. We verify the proposed PIFTNet on the dataset extracted from GitHub and empirical results prove the effectiveness of PIFTNet.
Characterization of a glycoside hydrolase endolysin from Acinetobacter baumannii phage AbTZA1 with high antibacterial potency and novel structural features
Authors: Premetis Georgios E. , Stathi Angeliki , Papageorgiou Anastassios C. , Labrou Nikolaos E. 
Published: November 2022

The classification of Acinetobacter baumannii by WHO as ‘priority 1’ antibiotic‐resistant pathogen underlines the urgent need for novel antimicrobial agents towards this pathogen. In this work, screening of the A. baumannii phage AbTZA1 genome allowed the identification of a putative endolysin (AbLys1, EC3.2.1.17) that belongs to the glycoside hydrolase family 24 (GH24). The sequence of AbLys1 was cloned, expressed in E. coli and purified. The lytic activity and specificity of AbLys1 were evaluated against a range of Gram‐positive and Gram‐negative human pathogens. AbLys1 was found to display a high selectivity towards A. baumannii. Kinetic analysis was carried out to characterize the dependence of its lytic activity on pH. The enzyme shows its maximal activity at pH values 7–8. The structure of AbLys1 was determined by X‐ray crystallography to 1.82 Å resolution. The overall structure revealed two helical domains: a small, antenna‐like, N‐terminal domain and a larger C‐terminal domain with six α‐helices and a β‐hairpin. Both the antenna‐like and β‐hairpin regions contain short sequences (AMseq1 and AMseq2) with predicted antimicrobial activity. Engineering studies revealed a key role of AMseq1 and AMseq2 on the enzyme's lytic activity towards A. baumannii cells but not towards purified peptidoglycan. This suggests that both sequences affect the destabilization of the outer membrane, thus providing access of the catalytic domain to the peptidoglycan. In addition, the deletion of AMseq1 enhanced the enzyme stability, whereas the deletion of AMseq2 diminished it. The results suggest that AbLys1 is a promising new enzybiotic with efficient lytic and antimicrobial activity.
Paradox versus paradigm: A disconnect between understanding and management of freshwater cyanobacterial harmful algae blooms
Authors: Bramburger Andrew J. , Filstrup Christopher T. , Reavie Euan D. , Sheik Cody S. , Haffner Gordon Douglas , Depew David C. , Downing John A. 
Published: November 2022

AbstractFreshwater cyanobacterial harmful algae blooms (cHABs) are a major threat to human and environmental health and are increasing globally in frequency and severity. To manage this threat in a timely manner, science must focus on increasing our ability to predict the growth and toxigenicity of specific taxa of cyanobacteria.Recent molecular research has revealed striking genomic and metabolic diversity among the many morphologically indistinguishable sub‐species and strains of cyanobacteria. Assemblage‐level molecular metabolic capability surveys promise to improve our ability to predict cyanobacterial responses to environmental forcing, but many of these cutting‐edge techniques are not widely available or cost‐effective enough to be employed in routine monitoring programmes to support management decisions. Taxonomic ambiguity, cryptic functional specialisation, incongruence between genomic capability and phylogeny, and genomic flexibility impose severe challenges to our ability to ascribe autecological attributes at a level of taxonomic resolution that is attainable under current management strategies (i.e. Linnaean species). This lack of knowledge prohibits reliable predictions of species' responses to environmental stressors.Cyanobacterial species comprise consortia of metabolically diverse, morphologically indistinct strains that span a range of ecological specialisation. Under current, broadly applied taxonomic concepts, these species functionally embody a generalist ecological strategy—persisting and/or proliferating where other specialised competitors are negatively impacted.We postulate that within current management frameworks, characterising of cyanobacterial species as competing generalists, as well as considering abundance trajectories of well‐characterised, non‐cyanobacterial specialist phytoplankton will generate more scalable, mechanistic, and management‐relevant insight into increasing cHAB frequency and severity in suitable time frames.Here we recommend that cHAB management considers the competitive framework of phytoplankton communities, including cyanobacteria, wherein diverse environmental changes lead to deterministic responses by readily identifiable, documented specialist taxa. Characterising these changes in community structure will quantify the relative importance of altered stressors and resource availability that can be exploited by metabolically flexible cyanobacteria.
Global diversity patterns of larger benthic foraminifera under future climate change
Authors: Förderer Esther‐Meena , Rödder Dennis , Langer Martin R. 
Published: November 2022

AbstractGlobal warming threatens the viability of tropical coral reefs and associated marine calcifiers, including symbiont‐bearing larger benthic foraminifera (LBF). The impacts of current climate change on LBF are debated because they were particularly diverse and abundant during past warm periods. Studies on the responses of selected LBF species to changing environmental conditions reveal varying results. Based on a comprehensive review of the scientific literature on LBF species occurrences, we applied species distribution modeling using Maxent to estimate present‐day and future species richness patterns on a global scale for the time periods 2040–2050 and 2090–2100. For our future projections, we focus on Representative Concentration Pathway 6.0 from the Intergovernmental Panel on Climate Change, which projects mean surface temperature changes of +2.2°C by the year 2100. Our results suggest that species richness in the Central Indo‐Pacific is two to three times higher than in the Bahamian ecoregion, which we have identified as the present‐day center of LBF diversity in the Atlantic. Our future predictions project a dramatic temperature‐driven decline in low‐latitude species richness and an increasing widening bimodal latitudinal pattern of species diversity. While the central Indo‐Pacific, now the stronghold of LBF diversity, is expected to be most pushed outside of the currently realized niches of most species, refugia may be largely preserved in the Atlantic. LBF species will face large‐scale non‐analogous climatic conditions compared to currently realized climate space in the near future, as reflected in the extensive areas of extrapolation, particularly in the Indo‐Pacific. Our study supports hypotheses that species richness and biogeographic patterns of LBF will fundamentally change under future climate conditions, possibly initiating a faunal turnover by the late 21st century.
Investigating the clinical decision‐making of nursing students using high‐fidelity simulation, observation and think aloud: A mixed methods research study
Authors: Abdulmohdi Naim , Mcvicar Andrew 
Published: November 2022

AbstractAimsThe aim of this study was to investigate nursing students' clinical decision‐making by using high‐fidelity simulation of a deteriorated patient scenario.DesignA convergent parallel mixed methods research design was used consisting of quantitative and qualitative data collection.MethodsTwenty‐three students completed the Health Science Reasoning Test before and after the simulation between October 2015 and June 2016. They were presented with a simulated scenario and asked to ‘think aloud’ during and after the simulation. The students were audio‐video recorded and observations were collected by the researcher.ResultsThere was a significant moderate increase in the ‘deduction’ and ‘analysis’ sub‐scale scores and overall test score, suggestive of improved analytical decision‐making processes through the simulation experience. Think‐aloud and observation data identified that students predominantly applied ‘forward’ reasoning during the simulated ‘patient's’ deterioration, focusing mainly on cue acquisition. ‘Backward’ reasoning with a focus on cue interpretation was most prominent in the debriefing data, in line with the survey outcomes. Accurate cue interpretation of critical, key cues appeared more useful than the total number of cues in solving the main clinical case problem.ConclusionStudents learn different clinical decision‐making skills during the simulation compared to what they learn from debriefing. Using observation and think‐aloud methods have significant benefits for researchers seeking to optimize the evaluation of the clinical decision‐making process.
Epidemiological study of capillary malformation among 7299 infants under 1 year of age in China
Authors: Yu Lei , Qin Kun , Deng Xianming , Yao Xiaojian , Deng Yuhong , He Wanying , Liu Qi‐wen , Tang Yichun , Yang Hong , Wang Zhengyuan , Zhu Zhaohua , Zhang Tangde 
Published: November 2022

AbstractBackgroundCapillary malformation (CM) is the most common vascular malformation. Large scale studies on its incidence and risk factors are limited in China.ObjectiveOur study aimed to investigate the incidence of CM in Chinese infants and to evaluate its potential risk factors.MethodsA cross‐sectional study, including 7299 infants (aged < 1 year) were collected by a self‐administered questionnaire. Independent‐samples T tests or χ2 tests and multivariable logistic models were used to examine the potential risk factors for CM.ResultsThe incidences of salmon patches and port‐wine stains (PWSs) were 9.10% and 0.80%, respectively. In analyses, male sex (OR: 1.32, 95% CI: 1.12–1.55) and birth hypoxia (OR: 5.61, 95% CI: 4.39–7.16) were risk factors for salmon patches. Birth hypoxia (OR: 12.58, 95% CI: 7.26–21.79) and pregnancy‐induced hypertension syndrome (PIH; OR: 3.66, 95% CI: 1.49–8.99) were associated with a higher risk of PWSs.ConclusionThis epidemiological study had the largest sample size of infants with CM in the world thus far, which updated its incidence in Chinese infants and found the potential risk factors for CM.
Drying kinetics of guabiju pulp (Myrcianthes pungens): Mass transfer parameters, mathematical modeling and evaluation of bioactive compounds
Authors: Bombana Vanessa Barbieri , Fischer Bruno , Oro Carolina Elisa Demaman , Rigo Diane , Polina Caroline Chies , Franco Denti Andressa , Gonçalves Tiago dos Santos , Steffens Clarice , Cansian Rogério Luis , Toniazzo Backes Geciane , Junges Alexander 
Published: November 2022

AbstractThis work aimed to estimate the mass transfer parameters of guabiju pulp under different temperatures of drying, modeling the kinetic curves using Fick's second law and the Lewis, Henderson–Pabis, Page, Modified Page, Logarithmic and Midilli models and to evaluate their biological properties and color. Guabiju pulp were dried at 313.15–373.15 K. The drying rate decreases over time, showing an exponential behavior with a significant influence of the operating temperature. Values of the effective diffusivity coefficient (Deff) between 1.578 and 10.755 × 10−9 m2 s−1were observed, with an activation energy of 30.77 kJ mol−1and an Arrhenius factor of 1962 × 10−4 m2 s−1. All models used showed good fit quality parameters (coefficient of determination—R2(>0.9521), sum of squared error—SSE (<0.0049), root mean square error—RMSE (<0.0699) and chi‐square test—X2(<0.0052)). The Midilli model showed the smallest deviations in relation to the experimental data. The values of the mass transfer constant (k6) ranged from 0.00036 to 0.01232 for the Midilli model. The best results for antioxidant activity, phenolic compounds and flavonoids were obtained at 353.15 K. The dehydrated guabiju pulp can be considered promising for human consumption and for food supplementation due to its biological activities, since the phenolic compounds act as pigments, flavor precursors and natural antioxidants.Practical applicationsDrying systems have proved to be an important strategy for the conservation of natural resources. However, they still lack studies by society. In line with drying systems, the kinetic modeling of the data allows simulating and optimizing the process, reaching the maximum potential of the process and making the process viable, reducing losses and errors and increasing product quality, facilitating industrial implementation and scale‐up. Thus, studies on the drying of guabiju pulp (Myrcianthes pungens) help to understand the process. Considering that the fruit has bioactive compounds and can be used in a certain product within the pharmaceutical, chemical or food industry.