bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circularity and biofertilizers in Agroforestry systems: impact on soil health
Authors: Álvarez-López Vanessa , Lado-Liñares Marcos , Lamas Alexandre , Vazquez Beatriz , Mosquera-Losada Rosa 
Published: May 2022

Abstract
Forest management practices impact the amount of organic matter reaching the soil, but also the soil properties and/or vegetation composition that influence soil microbiome structure and composition.
After 15 years of the last application of sewage sludge (SS) alone or in combination with CaCO3 to soils of a silvopastoral system with Pinus radiata D. Don, still some residual effects were observed in the soil chemical parameters (such as pH, exchangeable Na, Ca, or K) but bigger changes on soil bacterial families were detected. However, no significant effect of the fertilization was found in the total amount of soil organic matter or its mid-infrared spectral signature. Acidobacteriaceae, Sinobacteraceae, Bradyrhizobiaceae, Burkholderiaceae and Pseudonocardiaceae were the most abundant identified families in all treatments and light changes in their abundances were detected in the different soil treatments. However, when looking at the bacterial diversity (number of families) a strong decrease in the number of specific bacteria linked to the NF soils (Non fertilised) was observed in the SS treated soils (and this decrease was even more pronounced when SS was combined with CaCO3). Soil pH and exchangeable Al were found the main factors shaping the structure of bacterial families in the studied soils, while other factors such as total soil P, exchangeable Na, exchangeable K showed the lowest correlation with bacterial abundances. Most of correlations between soil pH and families’ abundances were negative while in the case of exchangeable Al, both negative and positive correlations were observed equally. The Thermomonosporaceae, Acidimicrobiaceae and Coriobacteriaceae families showed the highest number of significant correlations with soil chemical parameters (pH, total N, organic C, exchangeable K and Al and CEC).
Integrative analysis of the immune-related ceRNA network in fetal growth restriction based on weighted gene co-expression network analysis
Authors: Li Pingping , Zhao Yuebin 
Published: May 2022

Abstract
Purpose: Immune disorders lead to placental dysfunction and fetal growth restriction (FGR), but current research on the immune regulation mechanisms of FGR is insufficient. Therefore, this study aimed to construct an immune-related competing endogenous RNA (ceRNA) network to predict FGR onset risk. Methods: Based on microarray data from the GEO database, CIBERSORT was used to analyze immune cell infiltration. Weighted gene co-expression network analysis (WGCNA) was used to screen immune-related module genes with differential expression (DE) in FGR. A ceRNA network was constructed by integrating long non-coding RNA (lncRNA)-mRNA co-expression relations, lncRNA-microRNA (miRNA) relations, and miRNA-mRNA negative regulatory relations. The diagnostic values of key genes in the network and their relationships with immune cell infiltration were further validated. Results: By comparing FGR and normal samples, 442 DE mRNAs, 57 DE miRNAs, and 241 DE lncRNAs were obtained. Furthermore, four types of immune cells exhibited significantly different infiltration levels. WGCNA revealed 236 immune-related DE mRNAs that were involved in hormone secretion and immune cell differentiation. We then constructed a ceRNA network comprising 16 lncRNAs, 16 miRNAs, and 21 mRNAs through co-expression analysis and miRNA prediction. Receiver operating characteristic curves confirmed the superior predictive performance of key genes in the network for FGR. The validation dataset confirmed the increased infiltration of M1 macrophages in FGR samples and their positive correlations with NEURL1 and ODF3B expression. Conclusion: We constructed an immune-related ceRNA regulatory network wherein key genes are positively correlated with M1 macrophage infiltration and can be used for FGR diagnosis.
Identification of an epigenetic classification for hepatocellular carcinoma and biological relevance with cancer cells
Authors: Fu Yunong , Yang Kaibo , Wu Kunjin , Wang Hai , Li Qinglin , Zhang Fengping , Yang Kun , Yao Qing , Ma Xiaohua , Deng Yujie , Zhang Jingyao , Liu Chang , Qu Kai 
Published: May 2022

Abstract
Background: Hepatocellular carcinoma (HCC) is an extensive heterogeneous disease where epigenetic factors contribute to its pathogenesis. Polycomb group (PcG) proteins are a group of subunits constituting various macro molecular machines to regulate the epigenetic landscape, which contribute to cancer phenotype and have potential to develop molecular classification of HCC. Results: Here, based on multi-omics data analysis of DNA methylation, mRNA expression and copy number of PcG-related genes, we established an epigenetic classification system of HCC, which divides the HCC patients into two subgroups with a significantly different outcome. Comparing these two epigenetic subgroups, we identified different metabolic features, which were related to epigenetic regulation of Polycomb Repressive Complex 1/2 (PRC1/2). Furthermore, we experimentally proved that inhibition of PcG complexes enhanced lipid metabolism and reduced the capacity of HCC cells against glucose shortage. In addition, we validated the low chemotherapy sensitivity of HCC in Group A, and found inhibition of PRC1/2 promoted HCC cells sensitivity to oxaliplatin in vitro and in vivo. Finally, we found that aberrant upregulation CBX2 in Group A and upregulation of CBX2 was associated with poor prognosis in HCC patients. Furthermore, we found manipulation of CBX2 affected the levels of H3K27me3 and H2AK119ub. Conclusions: Our study provided a novel molecular classification system based on PcG-related genes data, and experimentally validated the biological features of HCC in two subgroups. Our founding supported the polycomb complex targeting strategy to inhibit HCC progression where CBX2 could be a feasible therapeutic target.
Resveratrol inhibits hepatic stellate cell activation by regulating autophagy and apoptosis through the SIRT1 and JNK signaling pathways
Authors: Zhang Jing , Ping Jian , Jiang Na , Xu Lieming 
Published: May 2022

Abstract
Resveratrol, a natural polyphenol found in grapes, berries, peanuts, and medicinal plants, has previously been reported to have several biological activities, including inhibiting hepatic fibrosis. Hepatic stellate cell (HSC) activation is the major cellular source of matrix protein-secreting myofibroblasts, which are the major drivers of liver fibrogenesis. Numerous studies on the protective effects of resveratrol against liver fibrosis have focused on the inhibition of HSC activation. Although the underlying mechanisms remain to be fully elucidated, autophagy and apoptosis regulation might be intimately related. The mouse HSC line JS1 was stimulated with resveratrol to assess the mechanism and relationship between autophagy and apoptosis. Resveratrol dose-dependently modulated JS1 cell viability. Moreover, resveratrol inhibited JS1 cell activation and induced autophagy and apoptosis. This antifibrotic effect was attenuated when autophagy was inhibited using chloroquine (CQ) or 3-methyladenine (3-MA) or when apoptosis was inhibited using Z-VAD-FMK. Furthermore, whether the Sirtuin1 (SIRT1) and c-Jun N-terminal kinase (JNK) signaling pathways were associated with resveratrol-induced autophagy and apoptosis in JS1 cells was examined. The SIRT1 inhibitor EX527 reversed autophagy, and the JNK inhibitor SP600125 was able to reverse both autophagy and apoptosis induced by resveratrol. These findings suggest that the SIRT1 and JNK signaling pathways may be involved in resveratrol-mediated inhibition of HSC activation by regulating autophagy and apoptosis.
Cardiotoxicity Associated With Pediatric Hodgkin Lymphoma Treatment
Authors: Mendes João , Francisco Andreia , Pires António 
Published: May 2022

Abstract
Introduction: Cardiotoxicity is one of the possible consequences of treating Hodgkin's lymphoma. This assumes a very important role in the patients outcomes since, increasingly, theyhave a higher survival rate, better quality of life and inherently longer survival. This is due to the improvement in the treatment of this pathology.Material and methods: Clinical information was collected from 103 patients about their age at diagnosis, sex, cardiovascular risk factors, anthracycline dose to which they were subjected and the use of mediastinal radiotherapy.According to this information, echocardiograms of these patients were analysed at five different times. Results: We obtained lower TAPSE values for females and for higher doses. Inverse relationships were also observed between the TAPSE value measured 6 months after treatment. The LV systolic function 5 years after treatment was inversed relationed with the age of the patients. Discussion: It was observed that there may be a relationship between the TAPSE 3 values and the anthracycline dose to which the patient was submitted, and we found lower TAPSE values in the group with the highest dose, in this case of 220 mg/m2.Conclusion: The incidence of cardiotoxicity after treatment for Hodgkin's lymphoma was 8% in the studied population.One of the conclusions we can take from this study is that it appears that cardiac toxic cardiac toxicity begins to reflect on right heart function in a chronic phase, more than 2 years after treatment.Other conclusions we can draw are that younger age, female sex and higher cumulative dose could be risk factors for cardiotoxicity due to anthracyclines.
Is testicular microlithiasis associated with testicular pathologies in children?
Authors: Sag Sefa , Elemen Levent , Masrabaci Kaan , Gungormez Esma Karadeniz 
Published: May 2022

Abstract
Purpose: To analyze the association of testicular pathologies with TM.Method: The retrospective study included pediatric patients who underwent scrotal ultrasonography (US) due to complaints including testicular pain, discomfort, swelling, scrotal redness in our clinic between June 2020 and January 2022. The patients were divided into two groups. Group 1; patients were diagnosed with testicular pathology or presented with testicular pain. Group 2; patients without testicular pathology or complaints. Patients were also classified as having undescended testis, epididymo-orchitis, varicocele and testicular pain without testicular pathology in group 1. Group 1 and subgroups of group 1 were compared with group 2 for the presence of TM.Results: A total of 516 patients were included in the study. Median age at the time of US examination was 24 months (range, 1 month - 17 years). There was no significant difference between groups 1 and 2, and boys with undescended testis and group 2 with regard to the presence of TM (p=0.85, p=0.55, respectively). TM was significantly higher in patients who had undergone orchiopexy and presented with testicular pain compared to group 2 (p=0.000, p=0.03, respectively). TM was not detected in patients with epididymo-orchitis, varicocele.Conclusion: We found no association between TM and testicular pathologies. Orchiopexy is likely to increase the prevalence of TM. Testicular pain may be a symptom of microlithiasis.
Analysis of the total serum levels of IgE levels in patients with acute exacerbations chronic obstructive pulmonary disease
Authors: Zheng Juan , Yuan Lindong , Li Zhen , Qi Jun , Li Lili , Jiang Tingting , Yang Ziyun , Qin Shan , Tian Xiufen , Wang Yan , Zhao Peige 
Published: May 2022

Abstract
BackgroundCurrently, no studies have demonstrated the relationship between IgE and AECOPD. Also, the utility of total serum IgE levels as a biomarker to guide AECOPD treatments remains to be determined.In this study, the total serum levels of IgE in patients with acute exacerbation chronic obstructive pulmonary disease (AECOPD) were investigated and correlated with the clinical characteristics.MethodsOur study retrospectively included AECOPD patients that were hospitalized at the Liaocheng People's Hospital from July 2018 to July 2019.we investigated the demographic variables, body mass index, smoking history, lung function, the frequency of acute exacerbation episodes in the past 12 months, the course of chronic obstructive pulmonary disease (COPD), medication history and complications. Routine blood tests, C-reactive protein levels and total IgE levels in the serum of patients were determined along with blood gas analysis. The length of hospital stay, mechanical ventilation during hospitalization, ICU admission, glucocorticoid administration, cumulative dose of glucocorticoid administration during hospitalization, duration of glucocorticoid administration, and the average dosage of glucocorticoid were also recorded. ResultsA total of 285 patients were included in the study that had a high proportion of males(74.1%). The proportion of patients with increased levels of T-IgE was 49.82%. According to the results of total IgE serum levels (> 60 kU/L as abnormal value), the patients were divided into high (n=142) and low high T-IgE groups (n=143). There was no significant difference in the dosage of glucocorticoid (including the number of patients receiving glucocorticoid, the average dosage of glucocorticoid, cumulative dosage and medication time) between the two groups. Patients in the high T-IgE group had shorter hospital stays and a lower probability of mechanical ventilation compared to the low T-IgE group. After adjustment for confounding factors, T-IgE was negatively correlated with the length of hospital stay. Univariate and multivariate Logistic regression analysis showed that low T-IgE was a statistically significant risk factor for mechanical ventilation and ICU admission in COPD patients.Conclusion Increased levels of t-IgE were found in many AECOPD patients suggesting that an allergic phenotype may be displayed in AECOPD. AECOPD patients with elevated t-IgE had shorter hospital stays and lower risks of mechanical ventilation and ICU admission. Our data show that t-IgE may have value as a biomarker in evaluating the condition of patients and guiding treatment decisions in AECOPD patients.
Establishing a Multimodal Imaging Tool pProTG for Selecting and Tracing Cells
Authors: Tian Yuan , XIAO Keyu , Yang Wenxiu , Zhang Peipei , Mou Yajun , Zhang Junhong , Li Qing , Yang Hui , Cui Yi , Hu Pingsheng , Dou Xiaowei 
Published: May 2022

Abstract
In order to select the cells in vitro, identify the selected cells and trace the selected cells in small animal, we establish a multimodal imaging tool pProTG. We fused enhanced green fluorescent protein, Puromycin N-acetyltransferase and Firefly Luciferase to construct a multimodal imaging tool pProTG, which have ability of selecting the positive cells by puroR, identifying the selected cells by EGFP and imaging the cells in small animal by Fluc. Our established multimodal imaging tool can be used to select the targeted cells in vitro and trace the cells in vivo.
COMSOL Simulation Study of Microwave Plasma in Cavity Resonator as a Transmitter-receiver Switch
Authors: Salem Mohammad Kazem , Yusefnejad Hadi , Zakri-Khatir Hadi 
Published: May 2022

Abstract
Microwave discharge characteristics and variable plasma conductivity play an important role in design of microwave filters and the efficiency of radars and electronic warfare devices. A three dimension model was designed to study the effect of plasma formation on high power microwave (HPM) transmittance in cavity resonator based on COMSOL platform. The effect of gap length, electrode shape and size, coaxial port dimensions and gas pressure, on the electric field in the gap and insertion loss in cavity were investigated. The effect of the cross-section of ​​ coaxial port on the electric field created in the gap was investigated and the best values ​​were calculated and determined. The electron density profile, electron temperature, electric conductivity, elastic and inelastic collision frequency were calculated. The performance of plasma limiter was optimized. Results were compared with the experimental data.
Dissemination of Information on Nuclear Incidents and Risks: ‘An assessment of the activity concentration level based on a survey of the Garigliano NPP.
Authors: ADEWOLE Olukorede , .  
Published: May 2022

Abstract
This explicit and full draft presentation reports the measurements done and analysis with key results,in - depth presentations, calculations and findings which as elaborated and concluded.The measured values and activity concentrations are obtained based on gamma spectroscopy of sediment samples drawn and obtained from the ‘Garigliano range and proximity of the ‘NPP up to depths of (0 – 70) m range as seen in the depth profile.The mean activity of K – 40 reported is 7,33E -01 Bq/g and 7,38E-01 Bq/g for sampled locations T0_DAT & T5_DAT respectively of sediment samples from the ‘Garigliano NPP.Cs – 137 as calculated and obtained shows a mean activity of 3.35E – 03 Bq/g and a standard deviation of 0.0034 (Bq/g) for sampled location T0_DAT.K – 40 activity showed depth dependence as presented and verified by the statistical testing from the hypothesis frame and formulation presented as explicitly discussed and demonstrated.The two sampled locations T0_DAT & T5_DAT can be said to have the same or equal means as tested and statistically affirmed from the hypothesis presented. This further justifies and inferring its ubiquitous nature and presence and the relatively high natural abundance of K – 40. ‘*Attention is also drawn and pointed to dissemination of information on nuclear risks and incidents while making references on reporting of nuclear incidents to the Fukushima and Chernobyl nuclear events and catastrophes as two key threatening and stunning moments in the history of nuclear events and disasters.The activity indexes have been reported, while assessing the status quo and making salient recommendations.
Assessment of Urban Deprivation Situation in Border-Settlements in Nigeria: The Lagos and Ogun Experience
Authors: OLOWOPOROKU Oluwaseun , DARAMOLA Oluwole 
Published: May 2022

Abstract

Understanding people’s experiences with their living condition is essential in reducing inequality in cities. This study examined urban deprivation in border settlements of Lagos and Ogun States with a view to providing information capable of enhancing the living condition of residents in the study area. Sample was selected using systematic sampling. Indexes tagged Residents Importance Index (RII) and Residents Satisfaction Index (RSI) were used to measure the importance residents attached to and the satisfaction residents derived from the physical, social and economic attributes of their settlements. The study revealed that residents in the border settlements of the two states experienced physical, social and economic form of urban deprivation. The study further revealed that across the border settlements of the two states, residents attached high importance to the facilities and services that culminated into the settlement’s attributes, however, they derived low satisfaction from them. Also, the study established that physical facilities across the two states were in poorest conditions compared to social and economic facilities and all this indicates a level of urban deprivation in the study area. The study recommended government intervention in the upgrading of the condition of existing facilities as well as introduction of public private partnership in the provision, upgrade and maintenance of public facilities in the study area.
G6PD promotes cell proliferation and dexamethasone resistance in multiple myeloma via increasing anti-oxidant production and activatingWnt/β-catenin pathway
Authors: Li Rui , Ke Mengying , Qi Mingming , Han Zhenru , Cao Yuhao , Deng Zhendong , Qian Jinjun , Yang Ye , Gu Chunyan 
Published: May 2022

Abstract
Glucose-6-phosphate dehydrogenase (G6PD) has been noticed for its deficiency causing a series of clinical diseases, however, the role of G6PD in cancer remains elusive. In this study, serum metabolic profiling identified that DHEAS level was higher in newly diagnosed multiple myeloma (MM) patients than in normal people. DHEAS can be converted into an endogenously uncompetitive inhibitor of G6PD known as DHEA. Clinical datasets from GEO showed an increasing expression of G6PD during MM progression, especially in those patients resistant to Dexamethasone (Dexa). We further investigated the oncogenic role of G6PD by using two MM cell lines in vitro and xenograft mouse model in vivo. Elevated G6PD promoted MM cell proliferation, in contrast silencing G6PD with RNAi or inhibitor suppressed MM cell growth and improved the survival of MM model mice. Mechanistically, high G6PD expression enhanced enzymatic generation of anti-oxidant NADPH via pentose phosphate pathway (PPP) and decreased the production of reactive oxygen species (ROS) thus inducing proliferation and Dexa resistance in MM cells. Furthermore, canonical Wnt/β-catenin signaling also participated in regulating G6PD-induced drug resistance and cellular redox level of ROS. Intriguingly, DHEA treatment could enhance the sensitivity of MM cells to Dexa mainly through augmenting cellular oxidative stress. In summary, G6PD enhances the enzymatic anti-oxidant NADPH generation and decreases ROS generation, thus encouraging MM cells resistant to Dexa-induced apoptosis mainly via enzymatic PPP pathway and non-enzymatic Wnt/β-catenin signaling pathway in MM. Targeting G6PD to harness cellular redox may afford a promising new strategy to improve therapeutic effect of MM patients.
Successful concomitant minimally invasive surgery for aortic valve stenosis and right lung cancer via right mini-thoracotomy : a case report
Authors: Sakakibara Satoshi , Nishi Hiroyuki , Fukui Shinya , Kitahara Mutsunori , Handa Kazuma , Kakizawa Yumi , Goto Takasumi , Funakoshi Yasunobu 
Published: May 2022

Abstract
BackgroundThe case of aortic valve stenosis complicated with lung cancer have compelled cardiovascular surgeons to make challenging. We report the first successful short-term outcomes of one-stage minimally invasive aortic valve replacement and video-assisted thoracoscopic surgery lobectomy through right mini-thoracotomy in a patient with synchronous bicuspid severe aortic valve stenosis which was unsuitable for transcatheter aortic valve implantation and right lung cancer.Case presentationA 76-year-old man with severe aortic valve stenosis was diagnosed with lung cancer of the right upper lobe. Considering the potential risk of tumor metastasis, a one-stage surgical therapy for right lung cancer and aortic valve stenosis was required; however, transcatheter aortic valve implantation was unsuitable due to a bicuspid aortic valve with severe calcification. Therefore, concomitant minimally invasive aortic valve replacement and lobectomy via right mini-thoracotomy were performed. The postoperative course was uneventful. ConclusionConcomitant aortic valve replacement and right lobectomy via right mini-thoracotomy may reduce surgical invasiveness, leading to early recovery. This surgical strategy is a useful option, particularly for patients with aortic valve stenosis complicated with right lung cancer.