bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simple production of resilin‐like protein hydrogels using the Brevibacillus secretory expression system and column‐free purification
Authors: Homma Toshimasa , Terui Shu , Yokoyama Fuki , Okino Saki , Ohta Sora , Kato Chihiro , Haraguchi Naoki , Fujisawa Ikuhide , Itsuno Shinichi , Ang Lily Zuin Ping 
Published: October 2022

AbstractResilin, an insect structural protein, has excellent flexibility, photocrosslinking properties, and temperature responsiveness. Recombinant resilin‐like proteins (RLPs) can be fabricated into three‐dimensional (3D) structures for use as cell culture substrates and highly elastic materials. A simplified, high‐yielding production process for RLPs is required for their widespread application. This study proposes a simple production process combining extracellular expression using Brevibacillus choshinensis (B. choshinensis) and rapid column‐free purification. Extracellular production was tested using four representative signal peptides; B. choshinensis was found to efficiently secrete Rec1, an RLP derived from Drosophila melanogaster, regardless of the type of signal peptide. However, it was suggested that Rec1 is altered by an increase in the pH of the culture medium associated with prolonged incubation. Production in a jar fermentor with controllable pH yielded 530 mg Rec1 per liter of culture medium, which is superior to productivity using other hosts. The secreted Rec1 was purified from the culture supernatant via (NH4)2SO4 and ethanol precipitations, and the purified Rec1 was applied to ring‐shaped 3D hydrogels. These results indicate that the combination of secretory production using B. choshinensis and column‐free purification can accelerate the further application of RLPs.
The real effects of ESG reporting and GRI standards on carbon mitigation: International evidence
Authors: Luo Le , Tang Qingliang 
Published: October 2022

AbstractThis study explores the real effects of environmental, social, and governance (ESG) reporting and the adoption of global reporting initiative (GRI) standards on carbon mitigation. Using a sample of large international companies, our empirical evidence does not show that a general form of ESG reporting is associated with carbon mitigation. However, after controlling for ESG reporting, firms that follow GRI standards when preparing their ESG reports are more likely to achieve greater carbon mitigation. Channel tests show that such firms tend to set more proactive carbon strategies and policies, make environmental investments, and actively engage with stakeholders. Finally, we find that the real effects of GRI standards tend to occur mainly in weak institutional settings (e.g., countries with less stringent carbon regulations, lower climate consciousness, and weaker legal enforcement). Overall, the central insight of the study is that standardized ESG reporting under GRI plays a soft substituting role in promoting green aspirations in an institution void of a high degree of global warming awareness.
“Watch out for the boogieman”: stigma and substance use recovery among migrants and ethnic minorities
Authors: Pouille Aline , De Ruysscher Clara , Vander Laenen Freya , Vanderplasschen Wouter 
Published: October 2022

AbstractPeople with a migration background and ethnic minorities (MEM) with substance use problems are confronted with stigma due to their ethnicity and substance use. This study explores how living with multiple stigmatized identities impacts substance use recovery among MEM. We conducted 34 in‐depth interviews with a diverse sample of MEM with substance use problems in Flanders, Belgium. A Qualitative Content Analysis using Earnshaw's stigma framework revealed that their recovery processes are challenged by accumulated stigma based on different intersectional statuses, namely ethnicity, substance use, psychological problems, criminal histories, poverty, unemployment and gender. These stigmas can negatively influence their well‐being, increase problem substance use and act as a barrier to recovery capital. While stigma moderators, which determine how stigma unfolds within society, were spread across the micro‐to‐macro continuum (from personal to social, community and macro‐cultural features), mediators that influence the role of stigma in recovery were mainly psychosocial. Hence, prevention and intervention strategies should be aimed at reducing stigma on the macro, meso and micro level, while individuals and communities can limit the impact of public stigma on recovery through constructive personal and collective coping and action mechanisms.
Cancer patients and targeted therapy during COVID‐19 pandemic: A descriptive case series study
Authors: Khodadadi Kasra , Moghimi Minoosh , Mansouri Reza 
Published: October 2022

AbstractTo evaluate the effectiveness of targeted therapy on preventing or treating COVID‐19, in this study, we want to share our experience about 14 patients (nine women, five men; average age, 59 years) who were treated with targeted therapy due to their underlying malignant disorders in our center.
Extremely rare combination of open dislocation of the ankle with superior type anterior hip dislocation without bone fracture: A case report
Authors: Joshi Padam Raj , Karki Shivaji , Timilsina Krishna , Adhikari Shiris , Bhandari Prawesh Singh , Uprety Suresh 
Published: October 2022

AbstractOpen dislocation of the ankle without associated fracture is extremely rare. Epstein's superior type of hip dislocation is the rarest type of hip dislocation. We reported a case of 50 years Nepalese gentleman who presented with a combination of these two rare orthopedic emergencies with the newly reported mechanism of injury by a garden tractor. Early extensive debridement of the wound with broad spectrum antibiotics coverage, reduction with delta frame external fixator, and postoperative rehabilitation helps in saving limb along with its function.
New‐onset atypical fulminant type 1 diabetes after COVID‐19 vaccination: A case report
Authors: Kobayashi Takaaki , Yakou Fumiyoshi , Saburi Masuo , Hirose Ai , Akaoka Hiroaki , Hirota Yusuke , Yunaiyama Daisuke , Asahi Nobuteru , Ohno Atsushi , Matsushita Takaya 
Published: October 2022

AbstractAdverse reactions, including the onset of diabetes, after coronavirus disease 2019 (COVID‐19) vaccination have been reported. Herein, we report a case of a man who developed anti‐glutamic acid decarboxylase (GAD) antibody‐positive fulminant type 1 diabetes 15 weeks after COVID‐19 vaccination, atypical of the previously reported anti‐GAD antibody‐negative cases.
A Small‐Molecule Probe with a Dual Function of miRNA Inhibition and Target identification
Authors: Wang Weishan , Deng Jiafang , Zhang Yan , Li Jinbo 
Published: October 2022

AbstractBy virtue of their key roles in pathologies, miRNAs represent a promising class of therapeutic targets. While high‐fidelity small‐molecule modulators of miRNAs can be identified via high‐throughput screening using cellular reporter systems, their modes of action are elusive due to the lack of proper tools. Here, we report a small‐molecule probe, 1 a, that is capable of elucidating its biological target along miRNA inhibition. Derived from norathyriol, a nature product, 1 a possessed a bioorthogonal alkyne moiety for subsequent labeling via copper‐catalyzed azide‐alkyne cycloaddition chemistry. We demonstrated that 1 a inhibited a panel of different miRNAs by blocking their loading onto argonaute 2 (AGO2), which is the key protein responsible for miRNA function. With the alkyne handle, we successfully identified AGO2 as an intracellular target of 1 a. Therefore, this work presents a novel small‐molecule tool for suppressing and probing miRNA regulatory pathways.
Modeling Type‐1 Iodothyronine Deiodinase with Peptide‐Based Aliphatic Diselenides: Potential Role of Highly Conserved His and Cys Residues as a General Acid Catalyst
Authors: Arai Kenta , Toba Haruka , Yamamoto Nozomi , Ito Mao , Mikami Rumi 
Published: October 2022

AbstractType‐1 iodothyronine deiodinase (ID‐1) catalyzes the reductive elimination of 5’‐I and 5‐I on the phenolic and tyrosyl rings of thyroxine (T4), respectively. Chemically verifying whether I atoms with different chemical properties undergo deiodination through a common mechanism is challenging. Herein, we report the modeling of ID‐1 using aliphatic diselenide (Se‐Se) and selenenylsulfide (Se‐S) compounds. Mechanistic investigations of deiodination using the ID‐1‐like reagents suggested that the 5’‐I and 5‐I deiodinations proceed via the same mechanism through an unstable intermediate containing a Se⋅⋅⋅I halogen bond between a selenolate anion, reductively produced from Se‐Se (or Se‐S) in the compound, and an I atom in T4. Moreover, imidazolium and thiol groups, which may act as general acid catalysts, promoted the heterolytic cleavage of the C‐I bond in the Se⋅⋅⋅I intermediate, which is the rate‐determining step, by donating a proton to the C atom.
Two Novel Three‐Dimensional Tetraphenylethylene‐Based Rare Earth MOFs with Ultra‐High Proton Conductivity and Performance Stability
Authors: Wang Shuyu , Zhao Lijia , Sun Hanxu , Wu Yuanyuan , Wang Ruidong , Zhang Suoshu , Du Lin , Zhao Qi‐Hua 
Published: October 2022

AbstractInvited for the cover of this issue are the groups of Lin Du and Qi‐Hua Zhao at Yunnan University. The image depicts astronauts as protons moving along the hydrogen‐bond network in the channel of Eu‐ETTB/Gd‐ETTB. Read the full text of the article at 10.1002/chem.202202154.
Process Intensification Using a One‐Step Inoculum Production and High‐Seeded Fed‐Batch Processes
Authors: Müller Jan , Ott Vivian , Weiss Noémi , Neubauer Peter , Eibl Dieter , Eibl Regine 
Published: October 2022

AbstractVarious approaches to process intensification are currently being investigated to ensure time and cost savings when producing biopharmaceuticals. In the present study, a one‐step inoculum production was established based on an ultra‐high cell density working cell bank with immunoglobulin G producing Chinese hamster ovary cells. Cryovials were used for direct inoculation of 1‐L wave‐mixed perfusion bioreactors. When reaching around 180 · 106 cells mL−1 in N−1 perfusion mode, low‐seed and high‐seed fed‐batch experiments in shake flasks were inoculated, as was the case for 250‐mL stirred single‐use bioreactors. Additionally, proof‐of‐concept runs at 50‐L and 200‐L scale were successfully performed. The intensification approach presented allows manufacturing capacity to be increased by up to 50 %.
Automatic structure recovery for generalized additive models
Authors: Shen Kai , Wu Yichao 
Published: October 2022

AbstractIn this article, we propose an automatic structure recovery method for generalized additive models (GAMs) by extending Wu and Stefanski's approach. In a similar vein, the proposed method is based on a local scoring algorithm coupled with local polynomial smoothing, along with a kernel‐based variable selection approach. Given a specific degree , the goal is to identify predictors contributing polynomially at different degrees up to and predictors that contribute beyond degree . By focusing on two GAMs, logistic regression and Poisson regression, we illustrate the performance of the proposed method using Monte Carlo simulation studies and two real data examples.
User experiences of structured stakeholder engagement to consider transferability: The TRANSFER approach
Authors: Munthe‐Kaas Heather , Nøkleby Heid , Rosenbaum Sarah 
Published: October 2022

AbstractBackgroundSystematic reviews are increasingly used to inform decision‐making in health, education, social care and environmental protection. However, decision makers still experience barriers to using reviews, including not knowing how findings might translate to their own contexts, and lack of collaboration with systematic review authors. The TRANSFER approach is a novel method that aims to support review authors to systematically and transparently collaborate with stakeholders to consider context and the transferability of review findings from the beginning of the review process. Such collaboration is intended to improve the usefulness and relevance of review findings for decision makers.ObjectivesWe aim to explore the user experience of the TRANSFER approach conversation guide, and in doing so gain a better understanding of the role and perceived value of stakeholder engagement in systematic reviews for informed decision‐making.MethodsWe conducted four user tests of groups using the guide, organized around simulated meetings between review authors and stakeholders. Review authors led the meeting using the TRANSFER approach conversation guide. We audio‐recorded and observed the meetings, collected feedback forms and conducted semi‐structured interviews with review authors following the meeting. We analysed the data using framework analysis to examine the user experience of the TRANSFER approach conversation guide and of stakeholder engagement more generally.ResultsSeventeen participants in four user groups participated in the user tests. Most participants were generally positive toward the structured approach using the conversation guide, and felt it would be useful in systematic review projects. We observed examples of misunderstanding of the terminology included in the guide, and received multiple suggestions for how to make the conversation guide more user friendly. We observed numerous challenges related to the hypothetical nature of a user test, including lack of familiarity with the review question/topic among participants and lack of preparation for the meeting.ConclusionsReview authors and stakeholders are positive toward using a structured approach to guide collaboration within the context of a systematic review. The TRANSFER conversation guide helps participants to discuss the review question and context in a structured way. Such structured collaboration could help to improve the usefulness and relevance of systematic reviews for decision making by improving the review question, inclusion criteria and consideration of transferability of review findings. The conversation guide needs to be modified to improve user experience. Further research is needed to explore stakeholder collaboration and the use of the TRANSFER conversation guide in systematic review processes.
Improving the Mechanical Properties of Bi2Te3‐based Thermoelectric Materials by K2Ti6O13 Whiskers
Authors: He Huolun , Wang Zhenting , Duan Sichen , Wang Xiaodong , Yin Li , Hou Shuaihang , Chen Chen , Liu Kejia , Wei Lihua , Sui Jiehe , Yang Bin , Zhang Shengnan , Mao Jun , Cao Feng , Zhang Qian 
Published: October 2022

AbstractMechanically robust thermoelectric materials are essential for designing reliable thermoelectric modules. The Bi2Te3‐based alloys show intrinsically poor mechanical properties, thus improving their mechanical performance is of great significance. In this work, the composites of p‐type Bi0.4Sb1.6Te3 and n‐type Bi2Te2.7Se0.3(CuI)0.003 with different K2Ti6O13 whiskers contents are prepared. Due to the grain refinement and the whiskers strengthening mechanism of crack deflection, whiskers pulling out, and whiskers bridging, the mechanical properties of Bi2Te3‐based materials are significantly improved. In addition, the zT value of the Bi2Te3‐based alloys remains almost unchanged compared with the pristine sample. Our study indicates that whiskers strengthening is an effective route to enhance the mechanical properties of thermoelectric materials.
Moving beyond economic criteria: Exploring the social impact of green innovation from the stakeholder management perspective
Authors: Tang Pengcheng , Liu Xuan , Hong Yao , Yang Shuwang 
Published: October 2022

AbstractUnlike previous studies mainly focused on the economic impact of green innovation, this paper explores its social returns, denoted by the stakeholder engagement, given stakeholders can affect or are affected by the firm's low‐carbon transition. Drawing on the complementary view and the substitute view, our empirical results from China over the sample period of 2012 to 2018 found that more green innovation would crowd in stakeholder‐related activities, especially for the welfare improvement of customers and suppliers. However, better stakeholder relations do not bring positive economic returns given the weak social pressures and uncertain rewards for being socially responsible in emerging countries. By doing so, our study expands the theoretical knowledge about the social impact of green innovation, thus offering a practical guidance for firms to simultaneously manage their environmental performance, social performance and economic performance in China and, potentially, in other emerging countries which also pursue environmental transition and better stakeholder management.
Rising cost of living creates a quandary for higher ed leaders
Authors: Arnett Autumn A. 
Published: October 2022

In her first week as president of Huston‐Tillotson University, Melva K. Williams, Ph.D., learned several of her faculty and staff were couch surfing, commuting long distances, and sleeping in their offices in the mornings and late afternoons while they waited out traffic to make the long drives back to their homes in places way outside of Austin's city limits. “They've got to do something to get that rent down,” Williams said of city officials.
Predictors of change in hypoglycemia unawareness among patients with type 1 diabetes
Authors: Melnychuk Jared , Wyne Kathleen , Dungan Kathleen 
Published: October 2022

AbstractAimsTo analyse predictors of hypoglycemia unawareness (HU) and improvement in Clarke score in clinical practice.Materials and MethodsWe retrospectively identified patients with type 1 diabetes (T1D) at an academic T1D clinic who completed HU surveys 6–12 months apart. HU (Clarke score ≥4) and improvement in Clarke score (decrease by ≥1 point or more clinically relevant ≥2 point) were assessed in univariable relationships and using multivariable logistic regression.ResultsOf the 300 participants, median diabetes duration was 19 years, 47 had HU at baseline, and 91 had an improvement by 1 point while 21 had an improvement by 2 points. Patients with baseline Clarke score ≥4 who had ≥1 or ≥2 point improvement had lower filtration rate (eGFR) than those who did not. After adjustment for other variables, gender (male OR 0.33, 95% CI 0.15, 0.74), log diabetes duration (OR 6.40, 95% CI 2.84, 14.5), and eGFR <60 ml/min/1.73 m2 (5.56, 95% CI 1.98, 15.6) were independent predictors of baseline HU. Continuous glucose monitoring use (OR 2.04, 95% CI 1.20, 3.48) and log diabetes duration (OR 1.78, 95% CI 1.22, 2.60) were independent predictors of 1 point improvement and eGFR <60 ml/min/1.73 m2 (OR 10.5, 95% CI 3.64, 30.0) and an education visit (OR 2.64, 95% CI 1.01, 6.89) were independent predictors of 2 point improvement in Clarke score.ConclusionsDiabetes duration, gender, and eGFR were independent predictors of HU. Improvement in Clarke score is possible in patients with long‐standing T1D, underscoring the need for additional study.
Titanosilicate ETS‐10 Molecular Sieve Anchored Silver Nanoparticles for Efficient Photocatalytic Degradation of Tetracycline Hydrochloride: Synergetic Effect of H2O2 and Ag/ETS‐10 under Visible Light
Authors: Sun Houxiang , He Xue , Zhang Huabing , Yang Haiyan , Zeng Chenghua , Yang Xueli , Dou Meiling , Li Yulong 
Published: October 2022

AbstractHerein, a facile and effective photocatalytic method for the degradation of the tetracycline hydrochloride (TCH) was proposed. The photocatalyst Ag/ETS‐10 was prepared by photoreduction, using cubic‐structured EST‐10 molecular sieves as the support for the silver nanoparticles. The synthesized photocatalyst Ag/ETS‐10 has small characteristic peaks and a chemical shift of Ag, resulting in a redshift. The results of photocatalytic experiments show that H2O2‐promoted Ag/ETS‐10 exhibits a superior visible‐light photocatalytic performance, and the degradation rate of TCH (50 mg L−1) is up to 94.9 % within 100 min, which is significantly better than pure ETS‐10 (23.9 %) and Ag/ETS‐10 (26.9 %). The reaction rate constant of H2O2‐promoted Ag/ETS‐10 is 17.75 and 31.55 times of those of ETS‐10 and Ag/ETS‐10, respectively. More importantly, the photocatalytic activity of H2O2‐promoted Ag/ETS‐10 remains stable after four cycles. The outstanding photocatalytic performance is due to the synergistic interaction of H2O2 with Ag/ETS‐10 to promote the rapid separation of photo‐generated electron‐hole pairs and enhance the generation of h+ and ⋅O2− radicals. In addition, the presence of silver induces the surface plasmon resonance effect and acts as a good acceptor for photogenerated e− in the photocatalytic reaction, enhancing the photocatalytic activities. This work provides a new insight for the design and synthesis of stable and efficient photocatalysts for visible light degradation of antibiotics.
Structure–Reactivity Relationship of Organozinc and Organozincate Reagents: Key Elements towards Molecular Understanding
Authors: Rio Jordan , Perrin Lionel , Payard Pierre‐Adrien 
Published: October 2022

AbstractThe main parameters that influence the structure–reactivity relationship of organozinc and organozincate reagents are gathered in this perspective article. Influences of substituents, inorganic salts, and solvents are discussed and illustrated with examples from the literature. Based on the peculiarities of this chemical system, the perspective is enlarged by molecular modelling considerations to raise the comprehension at the molecular scale of structure–activity relationships of organozinc and organozincate reagents in solution.
Enzymatic Synthesis of γ‐Glutamyl Dipeptides Catalysed by Mutant E. coli γ‐Glutamyltransferases
Authors: Rabuffetti Marco , Speranza Giovanna , Calvio Cinzia , Morelli Carlo F. 
Published: October 2022

Abstractγ‐Glutamyltransferases (GGTs) from different sources have been proposed in recent times as biocatalysts for the enzymatic synthesis of naturally occurring γ‐glutamyl derivatives with flavor‐enhancer properties and interesting biological activities. Although the enzymatic approach is considered as a viable alternative to both the troublesome and low‐yielding extraction from natural sources and synthesis through peptide chemistry requiring protection/deprotection steps, yields are not completely satisfactory, due to the intervention of GGT‐catalysed hydrolysis and autotranspeptidation side‐reactions. Here, the design and the use as biocatalyst for preparative purposes of two mutants of E. coli GGT are described. The design of mutants was pursued by docking‐guided identification of residues putatively involved in interaction with the acceptor substrate, thus probably representing a first identification of residues constituting the still elusive and poorly characterized acceptor substrate binding site of the enzyme.
Low‐Valent Cobalt‐Catalyzed Deprotection of Allyloxyarenes
Authors: Kokić Branislav , Selaković Života , Nikolić Andrea M. , Andrijević Ana , Anđelković Boban , Ajdačić Vladimir , Opsenica Igor M. 
Published: October 2022

AbstractHerein, we report a low‐valent cobalt‐catalyzed deprotection of allyloxyarenes. The method displays a broad substrate scope and good functional group tolerance, granting its utilization on more complex substrates, includingO‐allylated derivatives of natural products and drugs. Based on comprehensive experiments, a plausible mechanistic pathway for the low‐valent cobalt‐catalyzedO‐deallylation of allyloxyarenes is proposed.