bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural-vascular-functional correlation in type 2 non-proliferative macular telangiectasia
Authors: Venkatesh Ramesh , Reddy Nikitha Gurram , Mishra Pranjal , Yadav Naresh Kumar , Chhablani Jay 
Published: August 2022

Abstract
Purpose
To correlate the structural-vascular-functional changes in type 2 non-proliferative macular telangiectasia (MacTel) using optical coherence tomography (OCT) angiography (OCTA).

Methods
In this retrospective study, OCTA and enface OCT image analysis of eyes with confirmed diagnosis of non-proliferative type 2 MacTel was performed. The ‘MacTel area’ was calculated by marking the outer boundary of an area affected by MacTel on superficial (SCP) and deep capillary plexus (DCP) on OCTA images and photoreceptor layer (PRL) on enface OCT scan. At every follow-up OCTA scan visit, best-corrected visual acuity, MacTel area and stage of disease was documented. Analyses between disease stage, MacTel area and logMAR visual acuity was carried out.

Results
In total, 38 single-visit OCTA scans of 22 patients were included. The mean age was 58.9 ± 10.98 years. An increase in disease severity stage correlated positively with MacTel area in SCP segmentations slab (r = 0.334; p = 0.04) and logMAR visual acuity (r = 0.338; p = 0.038). No correlation in the DCP area or PRL area (p > 0.05) was noted with disease stage. A statistically significant positive correlation was noted between the structural changes in PRL layer with vascular changes in SCP (p = 0.021) but not in DCP (p = 0.199). No correlation of visual acuity with changes in SCP, DCP or PRL was noted (p > 0.05).

Conclusion
OCTA is a useful adjunct for determining disease severity in type 2 non-proliferative MacTel by assessing the structural-vascular changes. Further longitudinal studies need to be considered in future for understanding the pathomechanism of retinal damage in type 2 MacTel.
Validation of the English version of the arrhythmia-specific questionnaire in tachycardia and arrhythmia (ASTA): a Rasch evaluation study
Authors: Walfridsson Ulla , Walfridsson Håkan , Middeldorp Melissa E. , Sanders Prashanthan , Årestedt Kristofer 
Published: August 2022

Abstract
Background
Patient-reported outcome measures are important in person-centered care, providing valuable information about patients’ experiences. Disease-specific questionnaires add important information about a certain disease in comparison to generic questionnaires. Questionnaires need to be validated in the targeted population to achieve reliable data. The purpose with the study was to use Rasch measurement theory to evaluate the English version of the ASTA questionnaire.

Methods
The Rasch model theory was used to evaluate global and item fit, targeting, response category functioning, local independency, unidimensionality, differential item functioning (DIF) for gender and age, and reliability.

Results
The study included 202 patients undergoing DC conversion or catheter ablation at the Centre for Heart Rhythm Disorders at the University of Adelaide, Australia. The mean age was 67 years and 30% were women. Most patients had atrial fibrillation (n = 179), others had atrial flutter or had a combination. One of nine items demonstrated unsatisfactory model fit in the ASTA Symptom scale and two of 13 in the ASTA Health-Related Quality of Life (HRQoL) scale. Unidimensionality was supported for both scales. The targeting was acceptable except for the lower end of the scales. Both scales showed reversed thresholds for the response categories “quite a lot” and “a lot” (eight of ASTA symptoms and 12 of ASTA HRQoL items). Some problems with local dependency were detected in both scales. The reliability (person separation index) was satisfactory: 0.75 for the ASTA symptom scale and 0.77 for the ASTA HRQoL scale. No DIF for gender and age were detected.

Conclusions
The English version of the ASTA questionnaire demonstrated satisfactory measurement properties according to the Rasch model. However, it needs to be evaluated in patients with other arrhythmias. The response categories should be considered as well as DIF in further validation. The ASTA questionnaire can be used for assessments of symptoms and HRQoL between groups of different ages and genders in patients with arrhythmia.
Transarterial embolization of secondary inferior and superior mesenteric artery arteriovenous fistulas: a systematic review
Authors: Ruge Abhinandan , Kumar Nidhi , Jose Lija K. , Mahalmani Vidya 
Published: August 2022

Abstract
Background
Acquired arteriovenous fistula are rare complications of a piercing injury, including a gunshot or knife injury, the rupture of a preexisting aneurysm into a neighboring vein, or a complication of arterial catheterization or abdominal surgical techniques, most often colonic resection. Our aim was to develop an online systematic review of the literature to determine all documented incidents of endovascular embolization of secondary mesenteric Arteriovenous Fistula, spanning key databases as well as pertinent publications, from 1982 to 2019. Our case was included as well. The primary objectives were technical success and death; the secondary outcomes were the frequency of complications and the advice of the operators.

Main body
Forty articles, comprising 41 patients, including our own, were reviewed. The superior mesenteric vessels are the most commonly affected by fistulas. Endovascularembolization was used in 80% of the cases. 100% of the patients had technical success. The intraoperative death rate was 0%, with a 90-day mortality rate of 8%, half of which was unrelated to the original pathology. More than 60% of patients who underwent successful treatments had no severe problems. All others had uneventful recoveries, with only 12% experiencing mild problems that were managed conservatively.

Conclusions
The majority of authors supported this therapeutic approach thereby making endovascular glue embolization the treatment of choice for superior mesenteric arteriovenous fistula and inferior mesenteric arteriovenous fistula in high risk patients and in patients with multiple co-morbidities.
Sacituzumab Govitecan in Hormone Receptor–Positive/Human Epidermal Growth Factor Receptor 2–Negative Metastatic Breast Cancer
Authors: Rugo Hope S. , Bardia Aditya , Marmé Frederik , Cortes Javier , Schmid Peter , Loirat Delphine , Trédan Olivier , Ciruelos Eva , Dalenc Florence , Pardo Patricia Gómez , Jhaveri Komal L. , Delaney Rosemary , Fu Olivia , Lin Lanjia , Verret Wendy , Tolaney Sara M. 
Published: August 2022

PURPOSE Hormone receptor–positive (HR+) human epidermal growth factor receptor 2–negative (HER2–) endocrine-resistant metastatic breast cancer is treated with sequential single-agent chemotherapy with poor outcomes. Sacituzumab govitecan (SG) is a first-in-class antibody-drug conjugate with an SN-38 payload targeting trophoblast cell-surface antigen 2, an epithelial antigen expressed in breast cancer. METHODS In this global, randomized, phase III study, SG was compared with physician's choice chemotherapy (eribulin, vinorelbine, capecitabine, or gemcitabine) in endocrine-resistant, chemotherapy-treated HR+/HER2– locally recurrent inoperable or metastatic breast cancer. The primary end point was progression-free survival (PFS) by blinded independent central review. RESULTS Patients were randomly assigned to receive SG (n = 272) or chemotherapy (n = 271). The median age was 56 years, 95% had visceral metastases, and 99% had a prior cyclin-dependent kinase 4/6 inhibitor, with three median lines of chemotherapy for advanced disease. Primary end point was met with a 34% reduction in risk of progression or death (hazard ratio, 0.66 [95% CI, 0.53 to 0.83; P = .0003]). The median PFS was 5.5 months (95% CI, 4.2 to 7.0) with SG and 4.0 months (95% CI, 3.1 to 4.4) with chemotherapy; the PFS at 6 and 12 months was 46% (95% CI, 39 to 53) v 30% (95% CI, 24 to 37) and 21% (95% CI, 15 to 28) v 7% (95% CI, 3 to 14), respectively. Median overall survival (first planned interim analysis) was not yet mature (hazard ratio, 0.84; P = .14). Key grade ≥ 3 treatment-related adverse events (SG v chemotherapy) were neutropenia (51% v 38%) and diarrhea (9% v 1%). CONCLUSION SG demonstrated statistically significant PFS benefit over chemotherapy, with a manageable safety profile in patients with heavily pretreated, endocrine-resistant HR+/HER2– advanced breast cancer and limited treatment options.
Inflammatory Cytokines Rewire the Proinsulin Interaction Network in Human Islets
Authors: Tran Duc T , Pottekat Anita , Lee Kouta , Raghunathan Megha , Loguercio Salvatore , Mir Saiful A , Paton Adrienne W , Paton James C , Arvan Peter , Kaufman Randal J , Itkin-Ansari Pamela 
Published: August 2022

Abstract

Context
Aberrant biosynthesis and secretion of the insulin precursor proinsulin occurs in both type I and type II diabetes. Inflammatory cytokines are implicated in pancreatic islet stress and dysfunction in both forms of diabetes, but the mechanisms remain unclear.

Objective
We sought to determine the effect of the diabetes-associated cytokines on proinsulin folding, trafficking, secretion, and β-cell function.

Methods
Human islets were treated with interleukin-1β and interferon-γ for 48 hours, followed by analysis of interleukin-6, nitrite, proinsulin and insulin release, RNA sequencing, and unbiased profiling of the proinsulin interactome by affinity purification-mass spectrometry.

Results
Cytokine treatment induced secretion of interleukin-6, nitrites, and insulin, as well as aberrant release of proinsulin. RNA sequencing showed that cytokines upregulated genes involved in endoplasmic reticulum stress, and, consistent with this, affinity purification-mass spectrometry revealed cytokine induced proinsulin binding to multiple endoplasmic reticulum chaperones and oxidoreductases. Moreover, increased binding to the chaperone immunoglobulin binding protein was required to maintain proper proinsulin folding in the inflammatory environment. Cytokines also regulated novel interactions between proinsulin and type 1 and type 2 diabetes genome-wide association studies candidate proteins not previously known to interact with proinsulin (eg, Ataxin-2). Finally, cytokines induced proinsulin interactions with a cluster of microtubule motor proteins and chemical destabilization of microtubules with Nocodazole exacerbated cytokine induced proinsulin secretion.

Conclusion
Together, the data shed new light on mechanisms by which diabetes-associated cytokines dysregulate β-cell function. For the first time, we show that even short-term exposure to an inflammatory environment reshapes proinsulin interactions with critical chaperones and regulators of the secretory pathway.
Digitization of Symbol-Denoted Blood Pressure Data From Intraoperative Paper Health Records in a Low-Middle-Income Country Using Deep Image Segmentation and Associated Postoperative Outcomes: A Feasibility Study
Authors: Ndaribitse Christian , Durieux Marcel E. , Adorno William , Brown Donald E. , Tsang Siny , Naik Bhiken I. 
Published: August 2022

BACKGROUND:
In low-middle-income countries (LMICs), perioperative clinical information is almost universally collected on paper health records (PHRs). The lack of accessible digital databases limits LMICs in leveraging data to predict and improve patient outcomes after surgery. In this feasibility study, our aims were to: (1) determine the detection performance and prediction error of the U-Net deep image segmentation approach for digitization of hand-drawn blood pressure symbols from an image of the intraoperative PHRs and (2) evaluate the association between deep image segmentation-derived blood pressure parameters and postoperative mortality and length of stay.

METHODS:
A smartphone mHealth platform developed by our team was used to capture images of completed intraoperative PHRs. A 2-stage deep image segmentation modeling approach was used to create 2 separate segmentation masks for systolic blood pressure (SBP) and diastolic blood pressure (DBP). Iterative postprocessing was utilized to convert the segmentation mask results into numerical SBP and DBP values. Detection performance and prediction errors were evaluated for the U-Net models by comparison with ground-truth values. Using multivariate regression analysis, we investigated the association of deep image segmentation–derived blood pressure values, total time spent in predefined blood pressure ranges, and postoperative outcomes including in-hospital mortality and length of stay.

RESULTS:
A total of 350 intraoperative PHRs were imaged following surgery. Overall accuracy was 0.839 and 0.911 for SBP and DBP symbol detections, respectively. The mean error rate and standard deviation for the difference between the actual and predicted blood pressure values were 2.1 ± 4.9 and −0.8 ± 3.9 mm Hg for SBP and DBP, respectively. Using the U-Net model–derived blood pressures, minutes of time where DBP <50 mm Hg (odds ratio [OR], 1.03; CI, 1.01–1.05; P = .003) was associated with an increased in-hospital mortality. In addition, increased cumulative minutes of time with SBP between 80 and 90 mm Hg was significantly associated with a longer length of stay (incidence rate ratio, 1.02 [1.0–1.03]; P < .05), while increased cumulative minutes of time where SBP between 140 and 160 mm Hg was associated with a shorter length of stay (incidence rate ratio, 0.9 [0.96–0.99]; P < .05).

CONCLUSIONS:
In this study, we report our experience with a deep image segmentation model for digitization of symbol-denoted blood pressure from intraoperative anesthesia PHRs. Our data support further development of this novel approach to digitize PHRs from LMICs, to provide accessible, curated, and reproducible data for both quality improvement- and outcome-based research.
Focal adhesion kinase-mediated signaling controls the onset of pancreatic cell differentiation
Authors: Rodriguez Uylissa A. , Dahiya Shakti , Raymond Michelle L. , Gao Chenxi , Martins-Cargill Christina P. , Piganelli Jon D. , Gittes George K. , Hu Jing , Esni Farzad 
Published: August 2022

ABSTRACT
Signals from the endothelium play a pivotal role in pancreatic lineage commitment. As such, the fate of the epithelial cells relies heavily on the spatiotemporal recruitment of the endothelial cells to the embryonic pancreas. Although it is known that VEGFA secreted by the epithelium recruits the endothelial cells to the specific domains within the developing pancreas, the mechanism that controls the timing of such recruitment is poorly understood. Here, we have assessed the role of focal adhesion kinase (FAK) in mouse pancreatic development based on our observation that the presence of the enzymatically active form of FAK (pFAK) in the epithelial cells is inversely correlated with vessel recruitment. To study the role of FAK in the pancreas, we conditionally deleted the gene encoding focal adhesion kinase in the developing mouse pancreas. We found that homozygous deletion of Fak (Ptk2) during embryogenesis resulted in ectopic epithelial expression of VEGFA, abnormal endothelial recruitment and a delay in endocrine and acinar cell differentiation. The heterozygous mutants were born with no pancreatic phenotype but displayed gradual acinar atrophy due to cell polarity defects in exocrine cells. Together, our findings imply a role for FAK in controlling the timing of pancreatic lineage commitment and/or differentiation in the embryonic pancreas by preventing endothelial recruitment to the embryonic pancreatic epithelium.
Dictyostelium discoideum cells retain nutrients when the cells are about to outgrow their food source
Authors: Rijal Ramesh , Kirolos Sara A. , Rahman Ryan J. , Gomer Richard H. 
Published: August 2022

ABSTRACT
Dictyostelium discoideum is a unicellular eukaryote that eats bacteria, and eventually outgrows the bacteria. D. discoideum cells accumulate extracellular polyphosphate (polyP), and the polyP concentration increases as the local cell density increases. At high cell densities, the correspondingly high extracellular polyP concentrations allow cells to sense that they are about to outgrow their food supply and starve, causing the D. discoideum cells to inhibit their proliferation. In this report, we show that high extracellular polyP inhibits exocytosis of undigested or partially digested nutrients. PolyP decreases plasma membrane recycling and apparent cell membrane fluidity, and this requires the G protein-coupled polyP receptor GrlD, the polyphosphate kinase Ppk1 and the inositol hexakisphosphate kinase I6kA. PolyP alters protein contents in detergent-insoluble crude cytoskeletons, but does not significantly affect random cell motility, cell speed or F-actin levels. Together, these data suggest that D. discoideum cells use polyP as a signal to sense their local cell density and reduce cell membrane fluidity and membrane recycling, perhaps as a mechanism to retain ingested food when the cells are about to starve.
This article has an associated First Person interview with the first author of the paper.
Classifying fifty-seven Cucumis (Cucurbitaceae) accessions into six species using leaf architectural traits
Authors: MASUNGSONG LAILANI , Alcala Ailene , Buot Jr. Inocencio , Belarmino Marilyn 
Published: August 2022

Abstract. Masungsong LA, Alcala AA, Buot IE Jr., Belarmino MM. 2022. Classifying fifty-seven Cucumis (Cucurbitaceae) accessions into six species using leaf architectural traits. Biodiversitas 23: 4006-4017. In gene banks, there are myriads of accessions that need to be studied, grouped, classified and organized to the proper taxon and hence, be able to manage the accessions efficiently. Using leaf architecture traits, it is the objective of this study to determine the identity and classify the 57 Cucumis accessions in the genebank of Hortanova Farm and Research Center, East-West Seed Company, Inc., Lipa City, Batangas, Philippines. Five hundred thirteen Cucumis leaf samples across the 57 accessions were collected, measured, thoroughly investigated, and described using leaf architectural characters. Results of leaf architectural analyses focusing on the blade class, apex angle, secondary vein spacing, tertiary vein angle in relation to primary vein, and areole development, revealed that the 57 accessions can be grouped into six (6) species of Cucumis, namely, C. melo subsp. agrestis (Naudin) Pangalo, C. melo var. texanus Naudin, C. melo var. flexuosus (L.) Naudin, C. zambianus Widrl, J.H.Kirkbr., Ghebret. & K.R.Reitsma, C. sativus L., and C. sativus var. hardwickii (Royle) Gabaev. Cucumis sativus and C. sativus var hardwickii were delineated from the rest of the species for having a macrophyll blade class, an odd lobed acute apex angle and an obtuse tertiary vein angle in relation to the primary vein. This delineation is illustrated in the constructed dichotomous botanical key. Utilizing leaf architecture characters is an effective technique to characterize, identify and classify closely related taxa possessing confusing characters.
Morphological variations of superior sugarcane cultivars (Saccharum officinarum) from Lampung, Indonesia
Authors: WINDIYANI INTAN POESPITA , MAHFUT MAHFUT , PURNOMO PURNOMO , DARYONO BUDI SETIADI 
Published: August 2022

Abstract. Windiyani IP, Mahfut, Purnomo, Daryono BS. 2022. Morphological variations of superior sugarcane cultivars (Saccharum officinarum) from Lampung, Indonesia. Biodiversitas 23: 4110-4117. Sugarcane (Saccharum officinarum L.) is the main crop for producing sugar and for various industries. The need for sugar is continuously increasing as the population grows and the diverse demands of industrial needs. One of the efforts to increase sugar production is by developing high-yielding sugarcane varieties. In doing so, performing morphological characterization is necessary so that superior sugarcane cultivars can be identified. This study aimed to determine the morphological variations and phenetic relationships of the crosses of sugarcane cultivars of the collections of PT. Gunung Madu Plantation in Lampung, Indonesia. Morphological observation of 15 sugarcane cultivars was carried out on 37 characters covering leaves, leaf midrib, internodes, stems, eyes, and agronomical aspects. The dendrogram was built using the UPGMA cluster using the Gower’s General Similarity Coefficient algorithm, while the PCA analysis was performed using the Euclidean Distance algorithm using MVSP v.3.1 software. The results of the study showed that there were fairly high morphological variations among the sugarcane cultivars observed. The results showed the superior sugarcane cultivars of PT. GMP collections (i.e., GMP 1, GMP 2, GMP 3, GMP 4, GMP 5, GMP 6, and GMP 7) were not in one cluster due to differences between each parent. The principal component analysis of 37 characters showed that the total variation on axis I contributed 27.62% to the variation with an eigenvalue of 0.25, while axis II was 17.9% with an eigenvalue of 0.16. The information about morphological variations in this study suggests that morphological characters as taxonomic evidence are very good for identifying and analyzing local sugarcane cultivar variations and knowing the closeness of their kinship relationships.
Diversity and abundance of fruit flies (Diptera: Tephritidae) on different host plants in Depok and Bogor Districts, West Java, Indonesia
Authors: Tarno Hagus , Octavia Evy , Himawan Toto , Setiawan Yogo 
Published: August 2022

Abstract. Tarno H, Octavia E, Himawan T, Setiawan Y. 2022. Detection of fruit flies (Diptera: Tephritidae) Using Cue-Lure and Methyl Eugenol in Steiner Traps in Depok City and Bogor District, West Java, Indonesia. Biodiversitas 23: 4202-4208. Fruit flies (Diptera: Tephritidae) are one of the most economically important insect pests worldwide. Two types of attractants are recommended for monitoring and controlling fruit flies: methyl eugenol (ME) and cue-lure (CL). The objective of this study was to investigate the species richness and abundance of fruit flies (Diptera: Tephritidae) using methyl eugenol (ME) and cue-lure (CL) in Depok City and Bogor District, West Java, Indonesia. The study was conducted in three sites of fruit orchards in Bogor District i.e., Malay apple (Syzygium malaccense), Lemon (Citrus limon), Papaya (Carica papaya) and two sites of fruit orchards in Depok District i.e., carambola (Averrhoa carambola) and guavas (Psidium guajava). Male fruit flies were trapped using Steiner traps baited with methyl eugenol (ME) and cue-lure (CL). There were 11 fruit fly species collected from this study i.e., Bactrocera albistrigata, B. carambolae, B. dorsalis, B. neocognata, B. umbrosa, B. verbascifoliae, Dacus conopsoides, D. longicornis, Zeugodacus calumniata, Z. caudatus, and Z. cucurbitae. In this study, B. was , D. conopsoides, D. longicornis, and Z. caudatus were only collected in Steiner traps baited with CL and B. dorsalis and B. umbrosa also only responded to ME. During sampling periods, 20,217 male adult fruit flies were collected. The highest relative abundance attracted to ME was B. carambolae and CL was Z. cucurbitae. In this study, B. albistrigata, B. calumniate, D. longicornis, Z. caudatus and Z. cucurbitae were significantly more attracted to CL than ME. Whereas, B. carambolae, B. dorsalis, B. umbrosa, and B. verbascifoliae were significantly more attracted to ME than CL. Our study showed also did not find invasive species in Depok City and Bogor District, West Java, Indonesia. Our study can be used as a method for monitoring and controlling fruit flies, namely Steiner trap with CL for Z. cucurbitae and Steiner trap with ME for B. carambolae, B. dorsalis and B. umbrosa in Depok City and Bogor District, West Java, Indonesia.