bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluvastatin promotes chondrogenic differentiation of adipose-derived mesenchymal stem cells by inducing bone morphogenetic protein 2
Authors: Kuwahara Masanari , Akasaki Yukio , Goto Norio , Kurakazu Ichiro , Sueishi Takuya , Toya Masakazu , Uchida Taisuke , Tsutsui Tomoaki , Hirose Ryota , Tsushima Hidetoshi , Nakashima Yasuharu 
Published: August 2022

Abstract
Background
Adipose-derived mesenchymal stem cells (ADMSCs) are a promising source of material source for medical regeneration of cartilage. Growth factors, including transforming growth factor-β (TGFβ) subfamily members and bone morphogenetic proteins (BMPs), play important roles in inducing and promoting chondrogenic differentiation of MSCs. However, these exogenous growth factors have some drawbacks related to their cost, biological half-life, and safety for clinical application. Several studies have reported that statins, the competitive inhibitors of 3-hydroxy-2-methylglutaryl coenzyme A (HMG-CoA) reductase, induce the expression of BMP2 in multiple cell types as the pleotropic effects. The objective of this study was to investigate the effects of fluvastatin during chondrogenic differentiation of human ADMSCs (hADMSCs).

Methods
The effects of fluvastatin were analyzed during chondrogenic differentiation of hADMSCs in the pellet culture without exogenous growth factors by qRT-PCR and histology. For functional studies, Noggin, an antagonist of BMPs, mevalonic acid (MVA) and geranylgeranyl pyrophosphate (GGPP), metabolites of the mevalonate pathway, ROCK inhibitor (Y27632), or RAC1 inhibitor (NSC23766) were applied to cells during chondrogenic differentiation. Furthermore, RhoA activity was measured by RhoA pulldown assay during chondrogenic differentiation with or without fluvastatin. Statistically significant differences between groups were determined by Student’s t-test or the Tukey–Kramer test.

Results
Fluvastatin-treated cells expressed higher levels of BMP2, SOX9, ACAN, and COL2A1 than control cells, and accumulated higher levels of glycosaminoglycans (GAGs). Noggin significantly inhibited the fluvastatin-mediated upregulation of ACAN and COL2A1. Both MVA and GGPP suppressed the effects of fluvastatin on the expressions of BMP2, SOX9, ACAN, and COL2A1. Furthermore, fluvastatin suppressed the RhoA activity, and inhibition of RhoA–ROCK signaling by Y27632 increased the expressions of BMP2, SOX9, ACAN, and COL2A1, as well as fluvastatin.

Conclusions
Our results suggest that fluvastatin promotes chondrogenic differentiation of hADMSCs by inducing endogenous BMP2, and that one of the mechanisms underlying the effects is inhibition of RhoA–ROCK signaling via suppression of GGPP. Fluvastatin is a safe and low-cost compound that holds promise for use in transplantation of hADMSCs for cartilage regeneration.
Convolutional neural networks for automatic image quality control and EARL compliance of PET images
Authors: Pfaehler Elisabeth , Euba Daniela , Rinscheid Andreas , Hoekstra Otto S. , Zijlstra Josee , van Sluis Joyce , Brouwers Adrienne H. , Lapa Constantin , Boellaard Ronald 
Published: August 2022

Abstract
Background
Machine learning studies require a large number of images often obtained on different PET scanners. When merging these images, the use of harmonized images following EARL-standards is essential. However, when including retrospective images, EARL accreditation might not have been in place. The aim of this study was to develop a convolutional neural network (CNN) that can identify retrospectively if an image is EARL compliant and if it is meeting older or newer EARL-standards.

Materials and methods
96 PET images acquired on three PET/CT systems were included in the study. All images were reconstructed with the locally clinically preferred, EARL1, and EARL2 compliant reconstruction protocols. After image pre-processing, one CNN was trained to separate clinical and EARL compliant reconstructions. A second CNN was optimized to identify EARL1 and EARL2 compliant images. The accuracy of both CNNs was assessed using fivefold cross-validation. The CNNs were validated on 24 images acquired on a PET scanner not included in the training data. To assess the impact of image noise on the CNN decision, the 24 images were reconstructed with different scan durations.

Results
In the cross-validation, the first CNN classified all images correctly. When identifying EARL1 and EARL2 compliant images, the second CNN identified 100% EARL1 compliant and 85% EARL2 compliant images correctly. The accuracy in the independent dataset was comparable to the cross-validation accuracy. The scan duration had almost no impact on the results.

Conclusion
The two CNNs trained in this study can be used to retrospectively include images in a multi-center setting by, e.g., adding additional smoothing. This method is especially important for machine learning studies where the harmonization of images from different PET systems is essential.
Peripapillary structural and microvascular alterations in early dysthyroid optic neuropathy
Authors: Wu Yufei , Yang Qiaoli , Ding Liujun , Tu Yunhai , Deng Xiaoyu , Yang Yan , Shen Meixiao , Lu Qinkang , Lu Fan , Chen Qi 
Published: August 2022

Abstract
Background
To explore the changes in blood supply and structure around the optic nerve head (ONH) in thyroid-associated ophthalmopathy (TAO) patients with suspected dysthyroid optic neuropathy (DON).

Methods
TAO patients [19 with DON; 24 non-DON (NDON); 20 with equivocal DON (EDON)], and 34 control subjects were examined. Optical coherence tomography angiography (OCTA) was used to obtain peripapillary retinal nerve fiber layer (p-RNFL) and vessel density parameters, including the ONH whole image vessel density (ONH-wiVD) and the radial peripapillary capillary vessel density (RPC-VD) in early DON.

Results
Although there were no differences in p-RNFL thickness among the groups, there were differences in the ONH-wiVD of each grid section and the RPC-VD in all areas (P < 0.01). Compared with healthy controls, the EDON eyes had significantly lower RPC-VDs in all aeras (P < 0.05).The peripapillary region was further divided into eight sectors, and the RPC-VD in the temporal upper, superior temporal, and temporal lower sectors in the EDON group were significantly lower than in the controls. The visual impairment was closely related to the loss of peripapillary capillary vessel density. Univariate correlation analysis showed that the ONH-wiVD and RPC-VD of the TAO groups were negatively correlated with the intraocular pressure (r = − 0.296, P = 0.006; r = − 0.258, P = 0.016 respectively).

Conclusions
EDON patients had significantly lower ONH-wiVD and RPC-VD than control subjects, and the temporal and upper VDs were more likely to be affected in the early stage of TAO. The combined use of spectral domain optical coherence tomography and OCTA technologies offer a new method for early diagnosis of suspected DON patients.
A feasibility trial of prehabilitation before oesophagogastric cancer surgery using a multi-component home-based exercise programme: the ChemoFit study
Authors: Chmelo Jakub , Phillips Alexander W. , Greystoke Alastair , Charman Sarah J. , Avery Leah , Hallsworth Kate , Welford Jenny , Cooper Matthew , Sinclair Rhona C. F. 
Published: August 2022

Abstract
Background
Treatment for locally advanced oesophagogastric adenocarcinoma involves neoadjuvant chemotherapy which has a negative impact on patient fitness. Using ‘prehabilitation’ to increase activity levels and fitness may affect physiology, postoperative outcomes and improve patient wellbeing and quality of life. The aims of the trial were to address the feasibility and acceptability of recruiting participants to a home-based prehabilitation programme and provide data to allow design of future studies.

Methods
We recruited patients to a single-arm feasibility trial of home-based exercise prehabilitation. Eligible patients were aged ≥18years, had operable oesophageal or gastric adenocarcinoma and were receiving neoadjuvant chemotherapy at our tertiary referral hospital. All participants commenced a home-based exercise programme utilising pedometers and step counting to target daily aerobic exercise sessions alongside daily strengthening exercises. A weekly telephone consultation directed the exercise programme and facilitated weekly data collection. The primary (feasibility) outcomes for the trial were (a) recruitment rate, (b) completion rate, (c) engagement with the programme (use of pedometers, recording step counts, telephone consultations) and (d) compliance with exercise sessions, exercise intensity and strengthening exercises.

Results
There were 42 patients recruited, and the recruitment rate was 72.4% (42/58). 92.3% (36/39) of patients completed the exercise programme. There was 98.7% (IQR 93.2–100.0%) compliance with wearing a pedometer and recording data, and 100.0% (IQR 93.1–100.0%) compliance with a weekly telephone consultation. Exercise sessions and strengthening exercises were completed 70.2% (IQR 53.1–88.9%) and 69.4% (IQR 52.1–84.3%) of the time, respectively. Appropriate exercise intensity was recorded 96% (IQR 85.4–99.4%) of the time. There were no adverse events. Participants were enrolled in the exercise programme for a median of 91 days (IQR 84 to 105 days).

Conclusions
The results of this trial support the feasibility and acceptability of recruiting participants to an appropriately powered randomised controlled trial of prehabilitation.

Trial registration
Clinicaltrials.gov NCT04194463. Registered on 11th December 2019—retrospectively registered.
Early access to a cardio-oncology clinic in an Australian context: a qualitative exploration of patient experiences
Authors: White Jennifer , Byles Julie , Williams Trent , Untaru Rossana , Ngo Doan T. M. , Sverdlov Aaron L. 
Published: August 2022

Abstract
Background
Dedicated cardio-oncology services are emerging rapidly around the world in order to provide cardiovascular care (CV) for cancer patients. The perspectives of patients regarding their experience of cardiac surveillance during their cancer journey has not been qualitatively evaluated.

Methods
An interpretative qualitative study. Fifteen, in-depth qualitative interviews were conducted with a diverse range of community dwelling patients who attended a newly established cardio-oncology clinic in a large regional city in Australia. Data were analysed using an inductive thematic approach.

Results
Key themes were identified: (1) Access to a cardio-oncology clinic promotes information and understanding, (2) The experience of early CV intervention, (3) Factors promoting integrated care, (4) Balancing cancer treatment and CV symptoms and (5) Managing past and emerging CV risk factors.

Conclusion
As cardio oncology clinics continue to emerge, this study confirms the benefit of early access to a cardiologist for management of existing or emerging CV risk factors and diseases in the context of cancer treatment. Participants valued the opportunity for regular monitoring and management of CV issues that enabled them to continue cancer treatment. However, we identified gaps in education and support towards making positive lifestyle changes that reduce the risk of CV diseases in cancer patients.
Spontaneous hyperinflation of a giant bulla of the non-ventilated lung during laparoscopic cholecystectomy under one-lung ventilation: a case report
Authors: Mayahara Taku , Fukuoka Ryosuke , Shimada Norihiro , Nishiyama Junji 
Published: August 2022

AbstractBackgroundAnesthetic management of non-thoracic surgery in patients with giant bullae is challenging. We present a case of laparoscopic cholecystectomy in a patient with a giant bulla managed with one-lung ventilation (OLV).Case presentationA 75-year-old man with a giant bulla occupying the lower half of the right hemithorax underwent laparoscopic cholecystectomy. We managed anesthesia with OLV to avoid positive pressure ventilation of the giant bulla. Surgery was completed uneventfully; however, postoperative chest radiography indicated a large lucency occupying the entire right hemithorax. Although we suspected a pneumothorax due to a ruptured bulla, chest computed tomography (CT) led to a diagnosis of giant bulla hyperinflation. The giant bulla deflated gradually to its preoperative size within three postoperative days.ConclusionsManaging laparoscopic cholecystectomy in a patient with a giant bulla with OLV resulted in spontaneous hyperinflation of the giant bulla. Chest CT ruled out a pneumothorax.
Adoption of telehealth technologies: an approach to improving healthcare system
Authors: Sharma Arpana , Pruthi Madhu , Sageena Geetanjali 
Published: August 2022

Abstract
Background
Globally, the healthcare industry is well known to be one of the strongest drivers of economic growth and development. The sector has gained substantial attention to deal with the fallout of COVID-19, leading to improvement in the quality observed in developed and developing nations. With the advent of the twenty-first century, globalization an ever-growing populace, and environmental changes prompted the more noteworthy spread of irresistible diseases, highlighting the association between wellbeing and future health security. The massive spread of COVID-19 paralyzed the global economy and took a toll on health governance and wellbeing. The present review aims to map the harrowing impacts of COVID-19 on the QoL (quality of life) observed. Particularly the post-pandemic era is likely to boot-strap the healthcare sector. Hence in post COVID era, there is a dire need to strengthen the healthcare system and understand the evolving challenges to answer calls in recovery in the wake of COVID-19.

Conclusion
There is a flurry of research highlighting the implications faced due to the rise of the pandemic, resulting in the wrecking growth and development. However, the massive potential of telehealth is still largely underexplored with scarce research on countless evolving technologies. The current crisis highlighted the need to develop emerging frameworks and facilitate multilateral cooperation. The present research can serve as the baseline for better future strategies to improve global health initiatives. Further, this can help to focus on wider health determinants, redesign strategies and policies for the healthcare industry and to mitigate/deal better with future pandemics.
Altered brain activity in juvenile myoclonic epilepsy with a monotherapy: a resting-state fMRI study
Authors: Qin Linyuan , Zhang Yingying , Ren Jiechuan , Lei Du , Li Xiuli , Yang Tianhua , Gong Qiyong , Zhou Dong 
Published: August 2022

AbstractBackgroundJuvenile myoclonic epilepsy (JME) is the most common syndrome of idiopathic generalized epilepsy. Although resting-state functional magnetic resonance imaging (rs-fMRI) studies have found thalamocortical circuit dysfunction in patients with JME, the pathophysiological mechanism of JME remains unclear. In this study, we used three complementary parameters of rs-fMRI to investigate aberrant brain activity in JME patients in comparison to that of healthy controls.MethodsRs-fMRI and clinical data were acquired from 49 patients with JME undergoing monotherapy and 44 age- and sex-matched healthy controls. After fMRI data preprocessing, the fractional amplitude of low-frequency fluctuation (fALFF), regional homogeneity (ReHo), and degree centrality (DC) were calculated and compared between the two groups. Correlation analysis was conducted to explore the relationship between local brain abnormalities and clinical features in JME patients.ResultsCompared with the controls, the JME patients exhibited significantly decreased fALFF, ReHo and DC in the cerebellum, inferior parietal lobe, and visual cortex (including the fusiform and the lingual and middle occipital gyri), and increased DC in the right orbitofrontal cortex. In the JME patients, there were no regions with reduced ReHo compared to the controls. No significant correlation was observed between regional abnormalities of fALFF, ReHo or DC, and clinical features.ConclusionsWe demonstrated a wide range of abnormal functional activity in the brains of patients with JME, including the prefrontal cortex, visual cortex, default mode network, and cerebellum. The results suggest dysfunctions of the cerebello-cerebral circuits, which provide a clue on the potential pathogenesis of JME.
A framework for testing the impact of co-infections on host gut microbiomes
Authors: Schmid Dominik W. , Fackelmann Gloria , Wasimuddin  , Rakotondranary Jacques , Ratovonamana Yedidya R. , Montero B. Karina , Ganzhorn Jörg U. , Sommer Simone 
Published: August 2022

AbstractParasitic infections disturb gut microbial communities beyond their natural range of variation, possibly leading to dysbiosis. Yet it remains underappreciated that most infections are accompanied by one or more co-infections and their collective impact is largely unexplored. Here we developed a framework illustrating changes to the host gut microbiome following single infections, and build on it by describing the neutral, synergistic or antagonistic impacts on microbial α- and ß-diversity expected from co-infections. We tested the framework on microbiome data from a non-human primate population co-infected with helminths and Adenovirus, and matched patterns reported in published studies to the introduced framework. In this case study, α-diversity of co-infected Malagasy mouse lemurs (Microcebus griseorufus) did not differ in comparison with that of singly infected or uninfected individuals, even though community composition captured with ß-diversity metrices changed significantly. Explicitly, we record stochastic changes in dispersion, a sign of dysbiosis, following the Anna-Karenina principle rather than deterministic shifts in the microbial gut community. From the literature review and our case study, neutral and synergistic impacts emerged as common outcomes from co-infections, wherein both shifts and dispersion of microbial communities following co-infections were often more severe than after a single infection alone, but microbial α-diversity was not universally altered. Important functions of the microbiome may also suffer from such heavily altered, though no less species-rich microbial community. Lastly, we pose the hypothesis that the reshuffling of host-associated microbial communities due to the impact of various, often coinciding parasitic infections may become a source of novel or zoonotic diseases.
Effects of the COVID-19 pandemic on public bus occupancy and real-world tailpipe emissions of gaseous pollutants per passenger kilometer traveled
Authors: Dhital Narayan Babu , Wang Lin-Chi , Yang Hsi-Hsien , Lee Che-Hsuan , Shih Wei-Hung , Wu Cheng-Shiu 
Published: August 2022

AbstractPublic buses typically have less emission per passenger kilometer traveled (PKT) than private cars and motorcycles, and the emission benefit of public buses increases with ridership. However, the drop in public bus usage during the novel coronavirus (COVID-19) pandemic could lead to an increase in air pollutant emissions per PKT, making the emission benefits of public buses questionable. This study investigated the effects of the COVID-19 pandemic on public bus occupancy rates in Taichung City, Taiwan, and also compared real-world emissions per PKT of carbon monoxide (CO), total hydrocarbons (THC), nitric oxide (NO), and carbon dioxide (CO2) of a public bus before and during the pandemic. Mean bus occupancy rates were 11–25% on different bus routes before the pandemic, indicating that only a fourth or less of the bus passenger capacity was utilized. During the pandemic, mean bus occupancy rates dropped to 4–15%. Moreover, the public bus was less polluting based on CO and THC emissions than the car and motorcycle, even at the low passenger occupancy rates observed during the pandemic. However, NO and CO2 emissions per PKT of the bus were remarkably higher during the pandemic than those of the car and motorcycle. Furthermore, we estimated the break-even passenger occupancy rate for buses as 15%, which was the minimum threshold occupancy rate below which the buses would be more polluting than cars and motorcycles in terms of CO, THC, and CO2 emissions per PKT. Our findings will help transport management authorities and policymakers to optimize bus route designs and frequencies and implement anti-pandemic measures to maximize the environmental benefits of the public bus transit systems.
Benign paroxysmal positional vertigo: a multi-center study
Authors: El-Anwar Mohammad Waheed , Mesriga Rasha Mohamed Kamel Mostafa , Mobasher Mohamed Adel , Heggy Mohamed , Meky Abdelfatah , Nofal Ahmed Abdelfattah 
Published: August 2022

Abstract
Background
Benign paroxysmal positional vertigo (BPPV) is the most frequent vestibular disorder with significant morbidity, psychosocial impact, and medical costs. This multi-center study work aimed to review BPPV clinical features, treatment, and outcomes.

Methods
After a detailed history, clinical examination, audiological assessment, and position provocation tests to diagnose BPPV type, the suitable repositioning maneuver was done. BPPV was diagnosed according to the criteria developed for this study through piloting and validation in a specialized dizziness clinic. The main outcome measures were patient demographics, comorbidities, canal involvement, response to treatment, and incidence of recurrence.

Results
Within included 114 patients, the affected canal was 93% posterior semicircular canal, 3.5% horizontal semicircular canal, and 3.5% anterior semicircular canal. The response after repositioning maneuver was (86%) after one session and 100% after the second session.

Conclusion
Meticulous BBPV assessment with choosing appropriated provocation test help to diagnose and identify the type of BPPV and so applying appropriated suitable repositioning maneuver.
Controlling hydrocarbon dew point and water dew point of natural gas using Aspen HYSYS
Authors: El Maghraby M. A. , El Moniem N. A. , Abdelghany Amr 
Published: August 2022

AbstractA great attention is subjected to the environmentally friendly natural gas. Compared to other fossil fuels, natural gas is a cleaner burning due to the lower emission of carbon dioxides into the air. Removal of associated hydrocarbons from natural gas streams plays an important role to sell and to achieve the pipeline specification of natural gas. To satisfy the specification of pipelines, the hydrocarbon dew point and water dew point of natural gas must be controlled below the pipeline operating conditions to prevent many problems: two-phase flow and hydrate formation in the system. The main purpose of this paper is to simulate the gas plant process to study the effect of natural gas composition and changing in differential pressure of Joule–Thomson expansion valve on the obtained values of hydrocarbon, water dew points, and cricondentherm temperature.The results of process simulation using Aspen HYSYS have shown that the control of hydrocarbon dew point, water dew point, and cricondentherm of natural gas is achieved through increasing the Joule-Thomson valve differential pressure. There is an inverse relation between increasing Joule-Thomson valve differential pressure (∆p) and hydrocarbon dew point, water dew point, and cricondentherm to meet the specification of gas pipeline transmission. Increasing differential pressure (∆p) from 14 bar to 24 bar causes a decrease in hydrocarbon dew point, water dew point from −1 to −26°C and from 0 to −18°C, respectively. Cricondentherm is also decreased from 4 to −12°C by increasing differential pressure (∆p) from 14 to 24 bars. The operating conditions at differential pressure below 14 bar is not advisable because cricondentherm temperature does not meet the specification of gas pipeline transmission and hence lead to many problems. Careful adjustment of the operating conditions of gas processing plant is very important by making such simulations to choose the optimum operating conditions which meet gas pipeline transmission.