bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRISPR screens identify gene targets and drug repositioning opportunities at breast cancer risk loci
Authors: Rosenbluh Joseph , Tuano Natasha , Beesley Jonathan , Manning Murray , Shi Wei , Malaver-Ortega Luis , Paynter Jacob , Black Debra , Civitarese Andrew , McCue Karen , Hatzipantelis Aaron , Hillman Kristine , Kaufmann Susanne , Sivakumaran Haran , Polo Jose , Reddel Roger , Band Vimla , French Juliet , Edwards Stacey , Powell David , Chenevix-Trench Georgia 
Published: January 2022

Abstract
Genome-wide association studies (GWAS) have identified >200 loci associated with breast cancer (BC) risk. The majority of candidate causal variants (CCVs) are in non-coding regions and likely modulate cancer risk by regulating gene expression. However, pinpointing the exact target of the association and identifying the phenotype it mediates is a major challenge in the interpretation and translation of GWAS. Here, we used pooled CRISPR activation and suppression screens to evaluate predicted GWAS target genes, and to define the cancer phenotypes they mediate. We measured proliferation in 2D, 3D, and in immune-deficient mice, as well as the effect on DNA repair. We performed 60 CRISPR screens and identified 21 genes predicted with high confidence to be GWAS targets that drive a cancer phenotype by driving a proliferation or DNA damage response in breast cells. We validated the regulation of a subset of these genes by BC-risk variants, and show the utility of expression profiling for drug repurposing. We provide a platform for identifying gene targets of risk variants, and present a blueprint of interventions for BC risk reduction and treatment.
Porphyrin tripod as a monomeric building block for guest-induced reversible supramolecular polymerisation
Authors: Lee Hosoowi , Hwang Jun Ho , Lee Dajung , Kim Inhye , Lee Eunji , Jang Woo-Dong 
Published: January 2022

Abstract
Reversible supramolecular polymerisation and depolymerisation of biomacromolecules are common and fundamental phenomena in biological systems, which can be controlled by the selective modification of biomacromolecules through molecular recognition. Herein, a porphyrin tripod (DPZnT) connected through a triazole bridge was prepared as a monomeric building block for guest-induced supramolecular polymerisation. Although the lone pair electrons in triazolic nitrogen potentially bind to the zinc porphyrin units through axial ligation, the intrinsic steric hindrance suppressed the coordination of the triazole bridge to the porphyrin unit in DPZnT. Therefore, DPZnT formed spherical nanoparticles through π-π interactions. The addition of 1,3,5-tris(pyridine-4-yl)benzene (Py3B) caused the guest-induced fibrous supramolecular polymerisation of DPZnT by forming a 1:1 host-guest complex, which was further assembled into a fibrous polymer. Furthermore, addition of Cl− to DPZnT induced the transformation of spherical nanoparticles to fibrous supramolecular polymers. The fibrous supramolecular polymers of DPZnT obtained by adding Py3B or Cl− were depolymerised to their original spherical particles after adding Cu(ClO4)2 or AgNO3, respectively.
A More Complete Story of the Genetic Bases of Resistance to the Rice Hoja Blanca Virus
Authors: Silva Alexander , Montoya María Elker , Quintero Constanza , Cuasquer Juan , Tohme Joe , Graterol Eduardo , Cruz Maribel , Lorieux Mathias 
Published: January 2022

Abstract
Rice hoja blanca is one of the most serious diseases in rice growing areas in tropical Americas. Its causal agent is the Rice hoja blanca virus (RHBV), transmitted by the planthopper Tagosodes orizicolus Müir. Genetic resistance is the most effective and environment-friendly way of controlling the disease. So far, only one major quantitative trait locus (QTL) of Oryza sativa ssp. japonica origin, qHBV4.1, that alters incidence of the virus symptoms in two Colombian cultivars has been reported. This resistance has already started to be broken, stressing the urgent need for diversifying the resistance sources. In the present study we performed a search for new QTLs of O. sativa indica origin associated with RHBV resistance. We used four F2:3 segregating populations derived from indica resistant varieties crossed with a highly susceptible japonica pivot parent. Beside the standard method for measuring disease incidence, we developed a new method based on computer-assisted image processing to determine the affected leaf area (ALA) as a measure of symptoms severity. Based on the disease severity and incidence scores in the F3 families under greenhouse conditions, and SNP genotyping of the F2 individuals, we identified four new indica QTLs for RHBV resistance on rice chromosomes 4, 6 and 11, namely qHBV4.2WAS208, qHBV6.1PTB25, qHBV11.1 and qHBV11.2. We also confirmed the wide-range action of qHBV4.1. Among the five QTLs, qHBV4.1 and qHBV11.1 had the largest effects on incidence and severity, respectively. These results provide a more complete understanding of the genetic bases of RHBV resistance in the cultivated rice gene pool, and can be used to develop marker-aided breeding strategies to improve RHBV resistance. The power of joint- and meta- analyses allowed precise mapping and candidate genes identification, providing the basis for positional cloning of the two major QTLs qHBV4.1 and qHBV11.1.
Treponema Endosymbionts are the Dominant Bacterial Members with Ureolytic Potential in the Gut of the Wood-Feeding Termite, Reticulitermes Hesperus
Authors: Lin Jonathan Y , Huang Laibin , Won Sung J , Rodrigues Jorge L.M. 
Published: January 2022

Abstract
Termites are remarkable for their ability to digest cellulose from wood as their main energy source, but the extremely low nitrogen (N) content of their diet presents a major challenge for N acquisition. Besides the activity of N 2 -fixing bacteria in the gut, the recycling of N from waste products by symbiotic microbes as a complementary N-provisioning mechanism in termites remains poorly understood. In this study, we used a combination of high-throughput amplicon sequencing, quantitative PCR, and cultivation to characterize the microbial community capable of degrading urea, a common waste product, into ammonia in the guts of termites ( Reticulitermes hesperus ) from a wild and laboratory-reared colony. Taxonomic analysis indicated that a majority of the urease ( ureC ) genes in the termite gut (53.0%) matched with a Treponema endosymbiont of gut protists previously found in several other termites, suggesting an important contribution to the nutrition of essential cellulolytic protists. Furthermore, analysis of both the 16S rRNA and ureC amplicons revealed that the laboratory colony had decreased diversity and altered community composition for both prokaryotic and ureolytic microbial communities in the termite gut. Estimation by quantitative PCR showed that microbial ureC genes decreased in abundance in the laboratory-reared colony compared to the wild colony. In addition, most of our cultivated isolates appeared to originate from non-gut environments. Together, our results underscore a more important role for ureolysis by endosymbionts within protists than by free-swimming bacteria in the gut lumen of R. hesperus .
Myogenic Differentiation of Human Myoblasts and Mesenchymal Stromal Stem Cells under GDF11 on Poly-ɛ-Caprolactone-Collagen I-Polyethylene-Nanofibers
Authors: Cai Aijia , Schneider Paul , Zheng Zeng-Ming , Beier Justus P. , Himmler Marcus , Schubert Dirk W. , Weisbach Volker , Horch Raymund E. , Arkudas Andreas 
Published: January 2022

Abstract
Primary myoblasts (Mb) and adipose derived mesenchymal stromal cells (ADSC) can be co-cultured and myogenically differentiated in the process of skeletal muscle tissue engineering. Electrospun composite nanofiber scaffolds represent suitable matrices for tissue engineering of skeletal muscle, combining biocompatibility and stability. Although growth differentiation factor 11 (GDF11) has been proposed as a rejuvenating circulating factor, restoring skeletal muscle function in aging mice, some studies have also described a harming effect of GDF11.Therefore the aim of the study was to analyze the effect of GDF11 on co-cultures of Mb and ADSC on poly-ε-caprolacton (PCL)-collagen I-polyethylene oxide (PEO)-nanofibers.Human Mb were co-cultured with ADSC two-dimensionally (2D) as monolayers or three-dimensionally (3D) on aligned PCL-collagen I-PEO-nanofibers. Differentiation media were either serum-free with or without GDF11, or serum containing as in a conventional differentiation medium. Cell viability was higher after conventional myogenic differentiation compared to serum-free and serum-free + GDF11 differentiation as was creatine kinase activity. Immunofluorescence staining showed myosin heavy chain expression in all groups after 28 days of differentiation. Gene expression of myosin heavy chain (MYH2) increased after serum-free + GDF11 stimulation compared to serum-free stimulation alone. The results of this study show that PCL-collagen I-PEO-nanofibers represent a suitable matrix for 3D myogenic differentiation of Mb and ADSC. In this context, GDF11 seems to promote myogenic differentiation of Mb and ADSC co-cultures compared to serum-free differentiation without any evidence of a harming effect.
Comparison of ICSI and Conventional IVF in Non-Male Factor Patients with Less Than Four Oocytes
Authors: Isikoglu Mete , Ceviren Ayşe Kendirci Kendirci , Çetin Tuğba K , Avci Ayşenur , Aydınuraz Batu K , Akgül Özlem K Karabay , Karaca Mehmet K 
Published: January 2022

Abstract
Background: We aimed to analyse our clinical results for a particular subgroup of patients with poor ovarian response (POR) in order to clarify if lower number of oocytes is a drawback for proceeding to C-IVFMaterials and methods: In this retrospective study, patient files of all couples (#1733) who underwent oocyte retrieval between January 2017 and December 2019 were reviewed and 191 cases diagnosed with non-male factor infertility in which ≤3 cumulus-oocyte-complexes available for fertilisation were analysed. Exclusion criteria were: woman age>42, patients with a history of previous ART trial, prenatal genetic testing cycles and couples undergoing total cryopreservation for any indication. Three groups were constructed depending on the method of fertilisation and on semen quality as follows: IVF non-male factor (Group 1,n=77); ICSI non-male factor (Group 2, n=65); ICSI male factor- ICSI/MF n=49 according to WHO reference values. Main outcome parameters were: fertilisation rate, implantation rate and live birth rate. Results: Fertilisation rate per collected COC was significantly higher in group 1 compared to the other two groups (85,68%, 72,58%, 73,33% respectively, p=0,004). FR per inseminated oocyte also tended to be higher in group 1 but not reaching a statistically significant level. Both techniques yielded similar implantation rates (20,42%, 28,49%, 23,33% respectively, p=0,407) and live birth rates (26,8%, 30,6%, 31,1% respectively, p=0.643).Conclusions: In the presence of normal semen parameters, low egg number is not an indication to perform ICSI. The choice of fertilisation method should be based primarily on semen quality, in combination with the patient’s previous history regardless of the ovarian reserve.
Preparation and Ignition Properties of Tantalum Nitride Thin-Film Energy Transducers
Authors: Ren Xiaoming , Yu Kexin , Xie Ruizhen , Liu Lan , Liu Wei , Cheng Junxia , wu shenjiang 
Published: January 2022

Abstract
Tantalum nitride (TaN) has excellent electrical properties that can be used as an energy transducer in the ignition field. In this study, TaN film transducers with different bridge parameters were designed and fabricated in an attempt to reduce its energy consumption. The ignition sensitivity of the film transducers was tested using the Langley method. The results revealed that the ignition voltage is the lowest when the thickness of the film is 0.9 µm. If the thickness and length of the bridge film are fixed, the ignition voltage of the transducer first decreases and then increases with the width of the bridge film increases. When the thickness and width of the bridge film are fixed, the ignition voltage of the transducer is first decrease and then increase with the length of the bridge film increases. We also evaluated the ignition mechanism of TaN film transducers. By comparing the performance of TaN, semiconductor bridge (SCB), and nickel–chromium (Ni–Cr) film transducers, the TaN and SCB transducers are proven to have similar ignition performances, which are better than the Ni–Cr transducer. The negative temperature coefficient of TaN and the positive feedback after the initial electrothermal ignition promoted the growth and strengthening of plasma in the bridge film, allowing the medicament to ignite quickly. When the feasibility of the process and the influence of the bridge film parameters on ignition sensitivity are considered, the preferred design parameters of the transducer are a thickness of 0.9 µm and a bridge film size of 0.3 mm×0.3 mm. This study shows that TaN can be utilized as a high-performance transducer.
Open landfill site and threat to the proximity resident’s: Addressing perceived consequences of unscientific solid waste dumping using GIS techniques
Authors: Roy Subham , Bose Arghadeep , Basak Debanjan , Chowdhury Indrajit Roy 
Published: January 2022

Abstract
Municipal solid waste (MSW) disposal is a rapidly expanding sector that caters to the rising demand for disposal facilities; as a result, MSW treatment is becoming a significant challenge in concern to environmental and public health. The by-products of solid waste dumped in landfills have negative consequences on the environment and people living near disposal sites. This research aimed to assess whether the disposal of garbage in landfills affected the people who live near them. Consequently, residents living near MSW disposal facilities are exposed to various risks. A systematic questionnaire was devised and disseminated in this research to examine the adjacent people's concerns and attitudes around the landfill of the rapidly expanding Siliguri city of West Bengal. It was accomplished by assessing the opinions of persons living within the 1000m of the landfill site and how it impacts their life. The novelty of the research includes geographical analysis of physical impressions, including odour nuisance, breathing problems, impacts of flies and mosquitoes, water contamination, issues due to illegal burning, and health-related problems, which was conducted using geographic information system methodologies. Besides, the Landfill satisfaction index (LSI) tool was used to assess the perception of the people residing near the landfill. Also, to statistically validate the perception of the people related to the impact of landfills on their life, gamma coefficient (y) test of Goodman-Kruskal was applied. The findings reveal that the majority of the respondent within 600m are more susceptible to various risks due to unscientific landfill. Subsequently, a large portion of the responding sample was aware of the detrimental effects of landfills on the health and environment, therefore, individuals living near the dump yard preferred to live farther away. The findings also reveal that geographical proximity has a clear relationship between respondent perception and distance to the landfill. The study concludes with a discussion on how the research results may be beneficial for designing landfill sites and can be utilized by urban planners, environmentalists, and engineers.
Exploring Yak (Bos grunniens) Rumen Bacterial and Fungal Communities from 5 Days after Birth to Adulthood
Authors: Yang Shuli , Zhang Guangrong , Deng Mingyue , Sun Liyuan , He Shichun , Mao Huaming , Wu Dongwang 
Published: January 2022

Abstract
The gut microbial community of mammals, especially ruminants, plays an important role at different ages of the host. Ruminants have a unique compartment in their digestive tract; the rumen, comprising microorganisms that can effectively decompose plant fibers for the host to transform into milk and for growth and development, which is important for meat production. Colonization of rumen microorganisms is closely related to host developmental stage and affects host performance production. There is little information regarding initial colonization and subsequent changes of the rumen microbial population in wild grazing animals, from birth to adulthood. This study investigated the rumen bacterial and fungal populations of grazing yaks in five experimental groups, ranging from a few days after birth to adulthood using amplicon sequencing. Results indicated that rumen microbial communities of these yaks undergo a gradual change from 5 to 180 days after birth, with the bacterial and fungal diversity stabilizing at the age of 2 years. Additionally, Ruminococcus was detected in 5-day-old yak rumens, with a high percentage of Penicillium and other microbial species are important for normal rumen function detected in the adult rumen. The changes to the yak rumen microbial community after birth were reflected in the increased anaerobic fiber degradation group, and decreased aerobic and facultative anaerobic bacteria. Microbial diversity and abundance in the yak rumen increased with age. Rumen microbial composition of 6-month and 2-year-old yaks had obvious homogeneity. There were some differences in dominant rumen microorganisms among the different age groups. Further studies are required to confirm the functions of these differential and dominant microorganisms in each age group.
Malonic Acid-Type Fullerene Derivatives Strongly Inhibit The SARS-Cov-2 Main Protease
Authors: Katagishi Daiki , Yasuda Daisuke , Takahashi Kyoko , Nakamura Shigeo , Mashino Tadahiko , Ohe Tomoyuki 
Published: January 2022

Abstract
COVID-19 is a disease that is causing a global pandemic. There is an urgent need to develop new drugs to treat it. In this study, we evaluated the inhibitory activities of a series of fullerene derivatives against the main protease of SARS-CoV-2, the virus that causes COVID-19. As a result, it was found that the malonic acid-type fullerene derivatives showed strong inhibitory activities.
Application of magnetic method to characterize Abaya campus building site, Southern Ethiopia
Authors: Mulualem Abraham 
Published: January 2022

Abstract
A geophysical survey involving magnetic method is conducted using ENVI-MAG proton precision magnetometer. The study is carried out with the aim of identifying the possible geological structures which may responsible for the failure of engineering structures. Three traverses having east –west orientation with a ten meters profile spacing and a readings station spacing and one traverse across the three traverses and randomly collected magnetic data were used. At each station three readings were taken and averaged out in order to increase the accuracy of data and diurnal and geomagnetic corrections were made. With the corrected data different anomalous maps were produced like total magnetic field anomaly map, residual magnetic anomaly map and analytical signal map using Oasis montaji6.4 software for further interpretation. The result of this research has shown that the area is affected by different geological structures which may be the cause for the crack and the failure of the building in the area.
The GCDM Model
Authors: Johnson Martin Reid 
Published: January 2022

Abstract
A model for the reduction in Universal density over time, the “GCDM” model, is derived using gas thermodynamics with z = 1089 as the starting point. In the GCDM model, the Universe is pushing itself apart with internal gas pressure. A simple three-term Hubble expression HG is derived and found to be independent, or zero-order, in temperature and molecular weight of the gas. Isoentropic expansion of the gas at any z yields an entropic energy term which is modified to include energetic electrons, derived in turn from high-energy photons. These electrons are proposed as the source of the “dark energy” term found in the ΛCDM model.
A 12-month Randomised Pilot Trial of the Alzheimer’s and Music Therapy Study: A Feasibility Assessment of Music Therapy and Physical Activity in Patients with Mild-to-Moderate Alzheimer’s Disease
Authors: Matziorinis Anna Maria , Flo Birthe Kristin , Skouras Stavros , Dahle Kathrine , Sudmann Tobba Therkildsen , Gold Christian , Koelsch Stefan 
Published: January 2022

Abstract
Background: The Alzheimer’s and Music Therapy (ALMUTH) study is the first randomised controlled trial (RCT) design with 12 months of active non-pharmacological therapy (NPT) implementing music therapy (MT) and physical activity (PA) for participants with Alzheimer’s disease (AD). The aim of the present article is to retrospectively examine the inclusion of mild-to-moderate Alzheimer’s Disease patients into the main ALMUTH study protocol and to determine if continued inclusion of AD patients is warranted. Methods: The randomised pilot trial was conducted as a parallel three-arm RCT, reflecting the experimental design of the ALMUTH study. The trial was conducted in Bergen, Norway and randomisation (1:1:1) was performed by an external researcher. The study was open label and the experimental design features two active NPTs: MT and PA, and a passive control (no intervention, CON) in Norwegian speaking patients with AD who still live at home and could provide informed consent. Sessions were offered one time per week (up to 90 minutes) up to 40 sessions over the period of 12 months. Baseline and follow-up tests included a full neuropsychological test battery and three magnetic resonance imaging (MRI) measurements (structural, functional, and diffusion weighted imaging). Feasibility outcomes were assessed and were determined as feasible if they met the target criteria. Results: 18 participants with a diagnosis of mild-to-moderate AD were screened, randomised, and tested at baseline and after a 12-month follow-up interval. Participants were divided into three groups: MT (n=6), PA (n=6), and CON (n=6). Results of the study revealed that the ALMUTH protocol in patients with AD is not feasible. Adherence to protocol was poor (50% attended sessions), the presence of 50% attrition rates, 50% retention rates, insufficient and costly recruitment, issues with study fidelity, and many issues raised by staff. Recruitment status is still ongoing and the main study has been expanded to include milder forms of memory impairment. No adverse events were reported by the patients or their caregivers. Conclusions: The pilot trial was not deemed feasible in patients exclusively with AD. To mitigate this, the ALMUTH study has expanded the recruitment criteria to include participants with milder forms of memory impairment (pre-AD) in addition to expanding the neuropsychological test battery. The ALMUTH study is currently ongoing. Trial Registration: Norsk Forskningsråd (NFR) funded. Regional Committees for Medical and Health Research Ethics (REC-WEST: reference number 2018/206). ClinicalTrials.gov: NCT03444181 (registered retrospectively 23 February 2018, https://clinicaltrials.gov/ct2/show/NCT03444181).
Graviton in condensed photon sea
Authors: LEE JONG HOON 
Published: January 2022

Abstract
The ground-based device simulates the graviton explosion between gravity and magnetic seas. Trapped graviton was set to behave as free relativistic quantum particles, making it possible to induce magnetic fields as a function of time in the space Hieut (H). Our result is grounded on rigorous proof based on the photon sea for different initial superpositions of positive- negative-graviton spinor states. This explains that the interactive inducing protocol can be used to test the ability of the magnetic field not to communicate but to explode with relativistic quantum gravity.