bluuebunny/crossref_metadata_2025_split huggingface dataset:
METTL3 affects FLT3-ITD+ Acute Myeloid Leukemia by mediating autophagy by regulating PSMA3-AS1 stability
Authors: Wu Shenghao , Weng Shanshan , Zhou Wenjin , Chen Yuemiao , Liu Zhen 
Published: June 2022

Abstract
Objective
The study was designed to explore the role of PSMA3-AS1 in initiation and progression of acute myeloid leukemia (AML) and investigate its action mechanism.
Methods
Expression of PSMA3-AS1, miR-20a-5p and ATG16L1 both in vitro and in vivo was measured by qRT-PCR. The expression of protein was detected by western blot assay. Edu staining and flow cytometry were utilized to measure cell proliferation and apoptosis. Potential target was predicted by bioinformatics and was verified by dual-luciferase report gene assay and RNA pull down assay. QRT-PCR was used to quantify autophagy (LC3, Beclin1, P62) related genes. The m6A modification test is used to verify the effect of METTL3 on PSMA3-AS1. Tumor model was used to identify the effect of PSMA3-AS1 on tumor growth in vivo, and immunohistochemistry was applied to detect expression of ki67 and TUNEL.
Results
The results indicate that PSMA3-AS1 was upregulated in FLT3-ITD + AML patients. Si-PSMA3-AS1 can inhibit the proliferation, autophagy and inhibit the apotosis in MV4-11 cells. METTL3 could enhances the PSMA3-AS1 RNA stability. In addition, this study revealed that PSMA3-AS1 affected FLT3-ITD + AML by targeting expression of miR-20a-5p, and miR-20a-5p further modulated expression of ATG16L1, an mRNA that down-regulated in AML, to affect disease advancement.
Conclusion
PSMA3-AS1 could promote FLT3-ITD + AML progression by regulating the level of autophagy through the miR-20a-5p/ATG16L1 pathway. In addition, the increase of PSMA3-AS1 may be caused by the involvement of METTL3 in regulating its stability. This discovery will provide new horizons for early screening and targeted therapy of FLT3-ITD + AML.
Two Stages Active Disturbance Rejection Control for Coupled Permanent Magnet Synchronous Motors System with Mismatch Disturbance
Authors: Pu Chunmei , Wang Zhanshan , Wang Suran 
Published: June 2022

Abstract
Aiming at the disturbance mismatch in the coupled permanent magnet synchronous motors(CPMSMs) system, a new design method of active disturbance rejection controller(ADRC) is proposed in this paper. In order to eliminate the coupling tension between motors, the control law is designed in two stages, so that the disturbance and control input appear in the same channel, and then the ADRC is designed to realize the control goal by using the control input. The proposed method is more universal than the previous methods, and the parameter tuning is easier. At the same time, it simplifies the design steps of the controller and reduces the requirements of the control system.
Dynamics of plant nutrient requirements and acquisition strategies following afforestation: A study on Loess Plateau, China
Authors: Liu Weichao , Liu Yingyi , Wu Shaojun , Liu Fuhe , Wen Yuhao , Wang Yuanzhuo , Xia Kaila , Zuli Piye , Xu Yadong , Ren Chengjie , Han Xinhui 
Published: June 2022

Abstract
Aims
Plant nutrient requirements (NRs) and acquisition strategies are critical to understand the net primary productivity and community stability in terrestrial ecosystems; however, the current knowledge on this subject is limited, especially in cases of afforestation.
Methods
We selected one farmland and four Robinia pseudoacacia (RP) forests (15-, 21-, 31-, and 46-years-old) to investigate the changes in plant NRs and evaluate the effectiveness of acquisition strategies (including nutrient resorption and soil nutrient formed by mineralization) after afforestation in the Loess Plateau. We analyzed the nutrients in plant organs, litter, and soil, along with the extracellular enzyme activities (EEAs), microbial biomass, and mineralization rate. In addition, the biomass of RP was determined using an allometric growth model.
Results
During afforestation, the nitrogen and phosphorus requirement of RP increased significantly, from 3.97 to 18.14 g·m− 2·year− 1 and 0.17 to 0.71 g·m− 2·year− 1, respectively. However, the contributions of nutrient resorption to the total NR were lower than 50% and decreased significantly with stand age. Additionally, partial correlation analysis indicated that soil EEAs were the factors directly affecting the mineralization process. PLS-PM revealed that afforestation promotes the microbial biomass by increasing soil nutrients, leading to the secretion of more extracellular enzymes, which ultimately results in an increase in the soil mineralization rate.
Conclusions
Our study highlights the importance of soil mineralization for plant NRs and clarify the effects of afforestation on soil mineralization processes. Our study contributes to a better understanding of plantation nutrient cycling and, thus, facilitates efficient forest management practices.
Increased expression of miR146a dysregulates TLR2 signaling in airway epithelial cells from patients with chronic obstructive pulmonary disease
Authors: Reddy-Vari Hymavathi , Kim Yerin , Rajputh Charu , Sajjan Umadevi S 
Published: June 2022

Abstract
Toll-like receptors (TLR) play a pivotal role in stimulating innate immune responses of airway epithelial cells to bacterial and viral infections. Airway epithelial cells from patients with chronic obstructive pulmonary disease (COPD) show suboptimal responses to non-typeable H. influenzae (NTHi) and TLR2 ligands despite expressing TLR2 similar to normal airway epithelial cells, but the underlying mechanisms are not well-known. Here we demonstrate that compared to normal, mucociliary-differentiated COPD airway epithelial cell cultures show impaired expression of β-defensin (HBD2) when infected with NTHi or treated with TLR2 agonists. Apical secretions from TLR2 agonist-treated normal but not COPD airway epithelial cells efficiently kills NTHi. Knockdown of HBD2 significantly reduced NTHi killing by apical secretions of normal airway epithelial cells. Interestingly, compared to normal, COPD bronchial epithelial cell cultures showed a significant reduction in the expression of interleukin-1 receptor-associated kinase (IRAK)-1, a downstream component of TLR2 signaling. Attenuated IRAK-1 levels were associated with increased expression of microRNA (miR)146a. Treatment with miR146a antagonist increased the expression of IRAK-1 and improved the expression of HBD2 in response to TLR2 agonists in COPD airway epithelial cells. Further, inhibition of miR146a also enhanced the killing of bacteria by apical secretions of FSL-1-stimulated COPD airway epithelial cells. Reduced expression of IRAK-1 was also observed in the bronchial epithelium of COPD patients. These results suggest that reduced levels of IRAK-1 due to increased expression of miR146a may contribute to dysregulation of TLR2 signaling and impaired expression of HBD2 in COPD airway epithelial cells.
Multivariate time series traffic anomaly detection with Prediction &amp; AutoEncoder
Authors: Lee Junghyeon , Jeong Koosup , Kim Wooju 
Published: June 2022

Abstract
Recently, research on the detection of distributed Denial-of-Service(DDOS) abnormalities using network traffic data is actively underway. this is because malicious traffic continues to advance, causing great economic losses to oursociety. therefore, this study aims to build a more suitable model for use in thefield by detecting abnormalities distinct from normal and improving continuousabnormal detection accuracy based on multivariate traffic netflow data. the supervised learning method that directly utilizes data from the anomaly point of viewhas a fundamental limitation that it is difficult to respond to new types other thanpatterns as long as it is learned. Therefore, it was attempted to solve the problemthrough a semi-supervised learning method using only normal data. Therefore, wedesign a one class model structure that can improve classification performance fornormal and abnormal time points by applying a Multi-head Attention layer to theauto encoder layer. In addition, typical sequence-based anomaly detection modelshave recently become dominant, which can make it difficult to respond quickly toanomalies due to the normal points at the front of each sequence when appliedin the field. therefore, in order to supplement this, this study attempted to enable faster abnormal viewpoint detection by using a viewpoint prediction model through LSTM. In summary, we present a method to advance the performance ofthe model through an accurate and fast anomaly detection model by leveragingthe aforementioned reconstruction model and prediction model together.
Effect of Thoracoscopic-guided Paravertebral Block with Ropivacaine and Adjuvant Dexmedetomidine on the Early Postoperative Analgesia in Video-assisted Thoracoscopic Lobectomy: A Randomized Blinded Controlled Study
Authors: Li Zhixiong , Lin Qingshui , Lin Liangqing , Wu Qinghua , Ke Pinhui , Chen Huan , Lin Chunlan , Yu Yaohua 
Published: June 2022

Abstract
Background: Thoracoscopic-guided paravertebral block (PVB) has been increasingly popular as one component in multimodal analgesia. However, the optimal perioperative analgesic strategy for early postoperative analgesia following video-assisted thoracoscopic lobectomy (VATS LOBECTOMY) remains an open issue.Methods: A total of 106 patients who were scheduled for elective VATS LOBECTOMY were randomly assigned into two groups in a 1:1 ratio. All patients were given general anesthesia. The control group: patients received patient-controlled intravenous analgesia (PCIA) after surgery; PVB group: patients received thoracoscopic-guided thoracic 4-5 and 7-8 paravertebral blocks at the end of the surgery combined with PCIA after surgery. The primary endpoint was opioid consumption within 48 h after surgery. Secondary endpoints included the early postoperative pain scores assessed by Visual Analogue Scale (VAS) at rest and during exercising during 48h, duration of first-time usage of PCIA pump, times of analgesic pump pressing, postoperative quality of recovery (QoR) scores, incidence of postoperative pulmonary complications, delirium, nausea and vomiting (PONV).Results: In comparison with control group, sufentanil consumption was significantly lower for patients with thoracoscopic-guided PVB (p<0.001) with longer duration of first time PCIA usage (p<0.001), less times of analgesic pump pressing (p=0.034) and less rescue analgetic consumption (p=0.006). VAS scores at rest and during exercising were significantly lower in PVB group at all time points within the first 48 h after surgery. PVB group was also associated with significantly higher total QoR-40 scores during 48h after surgery. Significantly lower incidence of analgesia related adverse events including nausea and vomiting occurred in PVB group. However, there were no significant difference of major pulmonary complications between two groups.Conclusions: Thoracoscopic-guided PVB were effective and safe for early postoperative analgesia after video-assisted VATS LOBECTOMY. In addition, it could reduce the incidence of complications associated with analgesia, which might be recommended as an optimal analgesic strategy after VATS LOBECTOMY.Trial registration: The study was retrospectively registered in the Chinese Clinical Trial Registry (ChiCTR2200060675).
MAG - Cold Wire welding process with gas protection on the slave torch
Authors: Santos Deborah , Miranda Caio César , Megale Gustavo de Pádua , de Abreu Leonel Dimas , Júnior Pedro Américo Almeida Magalhães 
Published: June 2022

Abstract
This article explores the technical characteristics of welding processes and analyzes the structure of the welded joint (base metal, heat-affected zone, and weld metal) in terms of structure, mechanical properties, and penetration profile, using samples welded by the flux-cored arc welding (FCAW) and cold wire gas (CWG) processes. The evaluation of the system (welded joint) is based on the best weldability from tensile tests, analysis of the bead penetration profile, deposition rate, and heat added to the process. Welded joint performance comparisons are made using ABNT 1020 carbon steel with E-71T1 and E-70S6 wires, respectively. The specimens were welded in a flat position and chamfered, using different gas mixtures in both the FCAW and CWG processes. As a result, no discontinuity was observed in the welded joints, and the values of the mechanical properties tested did not indicate material embrittlement. Therefore, the values presented in the penetration profile and deposition rate were higher for the CWG process. The heat input in the welded joint was also lower for the CWG process, demonstrating a better performance for the process.
Impact of Telehealth in HIV Ambulatory Clinic during COVID-19 Pandemic
Authors: Nagarakanti Sandhya , Bishburg Eliahu , Ehlers Kristen 
Published: June 2022

Abstract
BackgroundThe COVID-19 pandemic has changed HIV outpatient care. Patients (pts) were transitioned from in-person (IN-P) visits to telehealth (TELE) encounters. We sought to evaluate the impact of TELE on HIV infected pts during the pandemic period (COV) and compare it to the pre-pandemic (pre-COV) care.MethodsA retrospective study in an outpatient HIV clinic. Two periods were defined: Pre-COV 3/16/2019 - 3/15/2020 and COV 3/16/2020- 3/15/2021. Data was collected on demographics, lab values and physician encounters. ResultsA total of 1136 pts were seen over 2 years, mean age 51.6 years. During pre-COV, 530 pts were seen IN-P and 606 pts had encounters during COV, of them 50.2% had exclusive TELE visits. The percentage of pts with CD4 > 200/µL and those with undetectable HIV VL at first and last encounter were compared between the two study periods. During pre-COV, there was no difference in the percent pts with CD4 > 200/µL (B90.5% vs. 94.3%, p=.87) or HIV VL (88.5% vs. 90.3%, p=0.41) but during COV there was significant difference in CD4 (90.3% vs. 94.2%, p=0.03) and HIV VL (87% vs. 93%, p=0.002). More new pts were seen and less changes to ART regimens were done during COV (8.6%vs 7 p=0.26) and (19% to 29%, p=0 .32) respectively.ConclusionsIn our clinic, transitioning to TELE resulted in larger number of encounters and more new pts entered care; CD4 and HIV viral load improvements were seen. TELE is an effective platform for HIV out patient care.
Evaluation of the Association between Blood Pressure and Birth Weight: A Cross-Sectional Study on 600 Outpatient Children
Authors: Ghandi Yazdan , Shabestari Ali Arjmand , Dorreh Fatemeh , YousefiChaijan Parsa , Rezaei Taraneh 
Published: June 2022

Abstract
BackgroundDespite the declining global prevalence of preterm birth, it accounts for a certain proportion of births in developing countries. Those born earlier, are prone to have lower birth weight (BW), smaller kidneys, lower glomerular filtration rate, higher blood pressure (BP) and overall poorer future cardio-metabolic health outcomes. MethodsThe current cross-sectional study was conducted on relatively healthy individuals aged 3-18 years with no PMH of any specific diseases, and not in toxic or ill condition. Recruitment was in 2 phases for the participants with high BP at the first visit (regarding a 2-week follow-up BP assessment) and in 1 phase for the normotensive participants.ResultsAmong the total 600 participants, the prevalence of elevated BP, grade 1 hypertension (HTN), and grade 2 HTN was 5.2, 5.5, and 2.3%, respectively. The prevalence of children with very low birth weight, low birth weight, and high birth weight was 1.7, 8.7, and 4.5%, respectively. Chi-square analysis showed no statistically significant association between BW and BP (P-value=0.774). There was a statistically significant association between BP and height, weight, and heart rate (HR) (P-value<0.05). ConclusionsThere is no statistically significant interaction between BP and BW. The association illustrated by previous studies may be caused by other underlying factors including weight or by methodological limitations including nor follow-up BP assessment, neither excluding ill, toxic, and hospitalized children. There is a direct relationship between BP and HR, weight, and height. However, the relationship between BP and the two latter is inverted for BP above grade 2 HTN.Trial RegistrationDesign of the current study was approved by the ethics committee of the Research Institute for Arak University of Medical Sciences. Ethics Code: IR.ARAKMU.REC.1400.271
Adsorption of Malachite Green Industrial Dye using Nanocellulose/Chitosan/ N, N-methylenebisacrylamide Based Nanocomposite Adsorbent
Authors: Goswami Rekha , Mishra Abhilasha , Prasad Brijesh , Bhatt Neha 
Published: June 2022

Abstract
Present study deals with the fabrication of nanocomposite adsorbent using nanocellulose extracted from lignocellulosic biomass (sugarcane bagasse) and appraise its removal efficiency for the malachite green (MG) dye. Chemically treated obtained cellulose nanocrystals (CNC) (5.0 g), Chitosan (0.5 g) and N, N-methylenebisacrylamide (MBA) (0.04 g) were applied as filler, binder and cross linker respectively for the synthesis of adsorbents nanocomposite. The nanocomposite was characterized for morphology, structure, presence and bonding of functional groups and Contact angle, Particle Size Analysis, wettability and its mechanical properties. Adsorption studies were done to evaluate dye adsorption capacity of nanocomposite adsorbent for different initial dye concentration. The results depicted the maximum dye removal rate in first 30 min and reached to equilibrium in 90 min. Other experimental conditions taken under considerations were adsorbent dosage (0.1 – 0.7 g), pH dependency (4.0 to 9.0) and initial dye concentration (20, 40 and 60 mg/L). Highest removal rate was observed with 5.0 % CNCs dosage after optimization. The experimental data showed the best fitted correlation with Freundlich isotherm (R2 = 0.99) and follow pseudo 2nd order reaction (R2 = 0.93) by enabling chemisorption adsorption phenomenon. The regeneration capacity of the nanocomposite was also analyzed it was found that about 90% of the dye was removed from the pre-adsorbed nanocomposite material. Taking the advantage of the developed nanocomposite it can be applied for removal of effluents specially from the paper, textile and leather industry and can be a cost-effective possible solution.
Measuring cancer burden in prostatic needle core biopsies: Simplified assessments outperform complex measurements in assessing outcome: evidence to assist pathologist efficiency and minimise datasets.
Authors: Berney Daniel , Finnegan Kier , Fine Samson , Varma Murali , Cuzick Jack , Beltran Luis 
Published: June 2022

Abstract
981 men with clinically localized prostate cancer diagnosed and managed conservatively were centrally reviewed by two uropathologists. Follow up was through cancer registries up until 2012. Deaths were divided into those from PC and those from other causes according to WHO criteria. For every case, the number of positive cores (NPC), the Maximum Cancer Length in a core (MCL), the total CL (TCL) and the percentage of positive cores (%+cores) was calculated and univariate and multivariate analysis using PSA, T-stage and Gleason score/Grade group were performed. In addition, the presence of stromal gaps (SG) was recorded and where present, length was assessed both including and excluding the SG. Univariate models were run on the patients where SG made a difference to the MCL.All variables of extent showed a significant association with prostate cancer death in the univariate models. In multivariate models, incorporating PSA, T-stage and Gleason score/Grade group, only %+cores was a significant predictor of outcome with a 10% increase in %+cores resulting in a hazard ratio (HR) of 1.07 (LRT p > Χ2 = 0.01). There were 120 patients where SG made a difference to the MCL and a total of 20 events in this group. Including SG, on univariate analysis the median MCL was 10 mm and HR was 1.16 (p=0.007), not including SG the median MCL was 6 mm and HR was 1.23 (p=6.3x10-4). Inclusion or exclusion of SG made no significant difference to TCL as a predictor of outcome.Cancer extent is a strong predictor of prostate cancer death but only %+cores adds to the multivariate model. Expressed as a fraction of NPC/total number of cores, this the simplest method of CE assessment which we favour over more complicated methods in non-targeted PB.
Bibliometric and Visual Analysis of Research on the Links between the Microbiome-Gut-Brain axis and Depression
Authors: Ma Yue , Xu Peng , Luo Yi , Sun jifei , GUO chunlei , fang jl 
Published: June 2022

Abstract
Background
The pathways and mechanisms underlying the associations between the gut microbiome and the brain are collectively known as the microbiome-gut-brain axis (MGBA). Depression is a common and frequent psychological and psychiatric disease. The interactions comprising the MGBA may play a role in the pathogenesis of depression. Nevertheless, the general aspects of the links between the MGBA and depression have not been systematically investigated through bibliometric analysis.
Methods
Publications from 1994 to 2022, focusing on the links between the MGBA and depression, were downloaded from the Web of Science Core Collection. HistCite, VOSviewer, CiteSpace, and the Bibliometrix Package and were used for bibliometric analysis and visualization. The main analyses we performed included collaboration network analysis, co-citation analysis, co-occurrence analysis, and citation burst detection.
Results
A total of 829 publications related to the relationship between the MGBA and depression were identified. The number of such publications has been rapidly growing since 2014. The People's Republic of China, University College Cork, and John F. Cryan were the most influential country, institute, and scholar, respectively. BRAIN BEHAV IMMUN and NUTRIENTS were the most productive and co-cited journals. Five hot topics in research linking the MGBA with depression were depression, gut microbiota, gut-brain axis, microbiota, and anxiety. Five frontier topics in the field were cytokine, maternal separation, neuroinflammation, probiotics, and vagus. The most representative and symbolic reference with the highest co-citation number was an article by Bravo J. A. et al. (2011).
Conclusions
These results provide an instructive perspective for research on the relationship between the MGBA and depression, and a timely review and analysis of research hotspots and research trends, which will promote the development of this field. Future research will focus on understanding the underlying mechanism of action of the MGBA on depression, and on elucidating microbial-based interventions and treatment strategies for depression.