bluuebunny/crossref_metadata_2025_split huggingface dataset:
Features of the Composition of Cytokines of Various Biological Effects in Patients with Uveal Melanoma: Local Changes in the Lacrimal Fluid. Part 1
Authors: Neroev V. V. , Saakyan S. V. , Katargina L. A. , Balatskaya N. V. , Kulikova I. G. , Myakoshina E. B. 
Published: April 2022

Purpose. Conduct an extended analysis of the cytokine status and its role in inflammatory processes in uveal melanoma based on multiplex analysis of the tear fluid.Patients and methods. Immunological studies of blood serum and lacrimal fluid were performed in 80 patients with uveal melanoma and 38 healthy donors (control group). The average age of the surveyed was 53.7 ± 12.2 years. Group 1 included 32 patients with small-sized choroidal melanoma (T1NoMo), group 2 consisted of 26 patients with medium-sized lesions (T2NoMo), group 3 — 22 patients with large tumor sizes (T3NoMo). The cytokine content in the tear fluid was determined by the method of multiplex analysis on the xMAP platform in the Luminex xPONENT 3.1 software using 47 plex kits (ProcartaPlex, eBioscience, Austria).Results. In the lacrimal fluid of the patient and the paired eye — an increase in pro-inflammatory (IL-1α, IL-2, IL-6, IL-17A, IL-18, TNF-α, MCP-1, MIP-1α, RANTES, GRO-α, IL-8, IP-10), anti-inflammatory (IL-1RA, IL-4, IL-10, IL-5, TGF-1β), proliferative (FGF-2, HGF, IL-15, PDGF-BB, Eotaxin), pro-tumor (NGF-β, IL-7), antitumor (IL-21), angiogenic (VEGF-A, SDF-1α) cytokines in the initial stage of uveal melanoma compared with the control group, p < 0.05.Conclusion. In response to the manifestation and growth of uveal melanoma, many chemoattractant mediators are produced, including pro-inflammatory and angiogenic effects, which promote tumor progression and affect the body’s immune response. The study of the lacrimal fluid proved the imbalance of the local immunity not only of the patient, but also of the paired «healthy eye». Research into cytokines leads to a deeper understanding of the pathophysiology of carcinogenesis and may contribute to the development of targeted therapies for inhibiting tumor growth.
Intraocular Lenses Optic Power Calculation in Extremely Short Eyes
Authors: Pershin К. B. , Pashinova N. F. , Likh I. A. , Tsygankov А. Yu. , Legkikh S. L. 
Published: April 2022

Purpose: to choose the optimal formula for calculating the IOL optical power in patients with an axial eye length of less than 20 mm.Patients and methods. A total of 78 patients (118 eyes) were included in the prospective study. Group I included 30 patients (52 eyes) with extremely short eyes (average axial eye length of 19.60 ± 0.42 (18.54–20.00) mm), group II consisted of 48 patients (66 eyes) with a normal axial length 22.75 ± 0.46 (22.00–23.77) mm with implantation of various monofocal IOL models. The average follow-up period was 13 months. IOL optical power was calculated using the SRK / T formula, retrospective comparison — according to the formulas Hoffer-Q, Holladay II, Olsen, Haigis and Barrett Universal II.Results. In group I, the maximum average estimation error was determined for the formula Haigis (0.88 ± 0.35), then for the formula Olsen, Barrett Universal II, Kane, SRK / T, Holladay 2 and Hoffer-Q (0.51 ± 0.12, 0.16 ± 0.38, 0.13 ± 0.28, 0.10 ± 0.59, 0.05 ± 0.54 and –0.12 ± 0.42, respectively). Similar data were obtained for the average absolute error — for the formulas Haigis, Olsen, Barrett Universal II, SRK / T, Holladay 2, Hoffer-Q and Kane, it was 0.85 ± 0.31, 0.78 ± 0.25, 0.21 ± 0.10, 0.79 ± 0.23, 0.73 ± 0.24, 0.19 ± 0.08 and 0.17 ± 0.06, respectively. When comparing the formulas, significant differences were found for the formulas Hoffer-Q, Barrett Universal II and Kane in comparison with the formulas Haigis, Olsen, SRK / T and Holladay II (p < 0.05) in all cases, respectively, which indicates the advantage of these formulas for the group patients with extremely short eyes. In group II, there were no significant differences between the studied formulas (p > 0.05).Conclusion. This paper presents an analysis of our own data on the effectiveness of six formulas for calculating the IOL optical power in extremely short (less than 20 mm) eyes in comparison with the normal axial length. The advantage of the Hoffer-Q, Barrett Universal II and Kane formulas over Haigis, Holladay 2, Olsen, and SRK / T is shown. To determine the exact indications for using these formulas, further studies are necessary taking into account the anterior chamber depth and a lesser degree of hyperopia.
Immediately Sequential Bilateral Cataract Surgery: the Opinion of Russian Ophthalmologists
Authors: Pokrovsky D. F. , Medvedev I. B. 
Published: April 2022

Purpose. A study of the attitude of Russian ophthalmologists to Immediately Sequential Bilateral Cataract Surgery (ISBCS).Methods. The study was carried out with the participation of 184 ophthalmologists actively performing cataract surgery, who were presented (in personal contact or via the Internet) a questionnaire developed by the authors, based on three main sections (“I use ISBCS in practice”; “I do not use ISBCS in practice”; “I don’t use, but I did ISBCS before”), which includes in each of the sections a number of clarifying questions and possible answers (“Doesn’t matter”; “Matters”; “Important”; “Very important”). At the same time, the analysis of possible answers was carried out by summing up two possible options — “Negative” opinion (“Not important” + “Matters”) and “Positive opinion” (“Important” + “Very important”). The age of the interviewed ranged from 26 to 58 years (mean age 36.2 ± 1.9 years), the experience of surgical activity in performing cataract phacoemulsification was predominantly (76 %) more than 5 years.Results. The data obtained indicate that 36 respondents (19.8 %) use ISBCS in their practice, 114 (61.8 %) do not use ISBCS; 34 (18.4 %) — do not use, but previously used ISBCS. At the same time, the volume of ISBCS performed on the same day is (for 84 % of respondents) no more than 20 % of the total number of operations performed. As part of the assessment of the main factors, in accordance with which (according to the ophthalmic surgeon), the patient is offered to perform ISBCS by the leader (in terms of the ratio of “positive” and “negative” opinions) is less clinic visits, and less time spent for the patient. The leading reasons for refusal to perform ISBCS by ophthalmic surgeons who do not use this technology are the possible occurrence of endophthalmitis, the risk of errors in IOL calculation, and medical and legal problems.Conclusion. The conducted questioning of domestic ophthalmologists testifies to the low prevalence of ISBCS technology, which is mainly associated with the possible risks of postoperative infectious complications, the lack of a standard protocol for surgical intervention, as well as medical and legal aspects. For the wider use of ISBCS, especially in a difficult epidemiological situation, it is necessary to carry out a set of measures of medical, educational and medical and economic orientation.
Clinico-bacteriological profile and antibiogram of Staphylococcus epidermidis with special emphasis on Methicillin resistance and hospital acquired infections in a tertiary care center south India
Authors: Nandihal Namratha W , Chikkaraddi Uma 
Published: April 2022

is a normal commensal of the skin and mucous membrane of humans and animals. Despite the growing importance of its pathogenesis especially in neonatal septicemia and device associated infections, it is still considered as insignificant isolate in the clinical practice. Hence, the present study is taken up to analyze the sources and risk factors of the isolates and to know their antibiogram along with occurrence of Methicillin resistant (MRSE).150 clinically significant isolates from various clinical specimens were considered in this study. Species identification was done by phenotypic methods. The antimicrobial susceptibility test and detection of Methicillin resistance were performed by Kirby-Bauer's disc diffusion method as per CLSI guidelines.Among 150 isolates, 78% were recovered from hospital acquired infections. They were commonly isolated in pediatric age group (30%) and among males (60.67%). Total of 34.67% were isolated from pus samples followed by blood (25.33%). Most of the isolates were associated with multiple risk factors like hospitalization, prior antibiotic administration, foreign body in situ and ICU admission. Majority of the isolates expressed resistance towards Penicillin (93.33%), followed by Amoxicillin-Clavulanic acid (76%), Cotrimoxazole (71.33%), Fluoroquinolones (64%), Gentamicin (60%) and Erythromycin (55.33%). Resistance against Amikacin (16.67%), Tetracycline (9.3%) and Linezolid (0.67%) was low. All isolates were sensitive to Vancomycin. Inducible Clindamycin resistance was 18% and MRSE was 68%.: Clinical importance and emergence of drug resistance among infections is growing with the advent of advanced medicine. This warrants the need to implement simple laboratory methods for species identification of the and to determine the antibiotic resistant patterns on routine basis. Clinical correlation of the isolate is crucial to rule out the colonizers and contaminants.
Seroprevalence and changing trend of dengue in a tertiary care hospital, Bhubaneswar, Odisha: Four-year retrospective study
Authors: Pathi Basanti Kumari , Poddar Nirmala , Panigrahi Kumudini , Pattnaik Dipti , Jena Jagadananda 
Published: April 2022

Dengue virus is a single stranded positive sense RNA virus belonging to the genus flavivirus family flaviviridae. Dengue fever is a seasonal and emerging acute mosquito borne arbo-viral illness affecting tropical and sub-tropical countries. This illness ranges from a mild asymptomatic form to severe dengue hemorrhagic fever (DHF) with or without dengue shock syndrome (DSS). This study was conducted to know the seroprevalence and changing trend of Dengue virus in a tertiary care hospital, Bhubaneswar, Odisha, India. Over a period of four years from August 2016 to December 2019, a total of 5147 blood samples from clinically suspected dengue patients were received in department of Microbiology laboratory. Serum was separated and subjected to enzyme immunoassay for detection of both Non Structural (NS1) antigen and IgM antibody.During this study period, a total of 5147 blood samples were processed from suspected dengue cases, out of which 1314(25%) samples were found to be positive by different serological markers like NS1 Antigen (Ag), IgM antibody(Ab), or both NS1 Ag & IgM Ab. The overall seroprevalence rate was found to be 25%. In this study period of four years, the year-wise seroprevalence rate was found to be 12% (153) in the year 2016 and was 26% (350) in the year 2017 and was 40% (522) in the year 2018, and was 22% (289) in the year 2019. It clearly shows that there is an increase in the dengue cases.
Bacteriological profile and antibiogram analysis from a tertiary care centre in eastern India: Time to Act / ReAct
Authors: Sharma Raj Kishor , Singh Asim , Ranjan Manish , Singh Mukesh Kumar 
Published: April 2022

Development of antimicrobial resistance imposes a serious threat to the timely and efficient healing of mankind who fall prey to the infectious agents. It is increasingly being reported day by day throughout the world. Irrational and overuse of antibiotics coupled with unavailability of regional or local antibiogram aggravates the situation further.To understand and present the local antbiogram data for this region and bridge the existing knowledge gap about the same for this particular region.Two year data was retrospectively analyzed from a 750 bedded tertiary care centre.A total of 1143 non repeat isolates were studied, their antibiogram profiles were analyzed. Percentage of Gram negative bacteria obtained were 59.6%, while Gram Positive bacteria were obtained in 40.4% of the isolates. (22.8%) was the most common organism isolated, followed by Staphylococcus aureus (14.2%), Other sp(12.8%), sp(12.6%), (9.4%), and complex(7.4%), Miscellaneous Bacteria(13.4%). An alarming number of isolates were found to be Multidrug resistant isolates(MDRs). With the panel of antibiotics tested for Gram negative bacterial isolates, only Fosfomycin(1.5%), Nitrofurantoin(16%) & Chloramphenicol (19%) were the only antibiotics to exhibit &#60;20% resistance. Antibiotics for Gram positive bacteria for which low resistance was observed were Vancomycin (0%), Linezolid (0.1%), Teicoplanin (&#60;1%), Aminoglycosides (18%), Nitrofurantoin(15%), Chloramphenicol(6%) & Tetracyclines(11%). Extended Spectrum Beta lactamase(ESBL) prevalence was estimated to be 63.6% in , 82.4% in Klebsiellapneumoniae. Methicillin resistant Staphylococcus aureus (MRSA) was seen in 54% of the isolates, while Inducible clindamycin resistance was observed in 35.9% of the isolates.It is high time that we shift our focus from internationally and nationally available data on antibiogram to locally prevalent antibiotic resistance pattern. It will ensure faster patient recovery at a lesser cost and also prevent undue development of drug resistance.
​​Evaluation of Cluster Bean [Cyamopsis tetragonoloba (L.) Taub] Genotypes for Drought Stress Adaptation and its Effects on Yield
Authors: Patel Samarth R. , Th Amarjeet Singh , Kumar Sushil , Fougat Ranbir S. 
Published: April 2022

Background: Cluster bean is mostly grown in dry land arid zone areas for various purposes as vegetable, seed, fodder and other industrial uses because of its gum presence in endosperm. Methods: An experiment was conducted under the rain out shelter for the evaluation of drought adaptability of selected 10 genotypes of cluster bean. The physiological parameters were used to screen the drought resistance and also recorded various yield and yield attributing parameters at the time of harvesting. Result: Among the genotypes, Pusa Navbahar was observed well adaptability having adequate relative water content, maximum chlorophyll content, stomata per unit area and higher dry matter accumulation followed by genotype IC116866. Furthermore, yield and yield attributing parameters also showed significant differences; Pusa Navbahar was recorded maximum plant height (188.6 cm), leaves per plant (173.9), pods per plant (123.9), pod length (7.1 cm) and test weight (14.3 g) and well adaptability of primary and secondary root with deep in nature under the drought stress among the genotypes. Therefore, this study would be very helpful in the proper selection of genotypes as a source of drought resistance lines in future breeding and crop improvement programmes.
MENGAQIQAHKAN ORANG YANG SUDAH MENINGGAL MENURUT MADZHAB SYAFI’I DAN MADZHAB HANBALI
Authors: Gunawan Fauri , Purnomo Bitoh 
Published: April 2022

Abstract
The Law of Imparting People Who Have Died According to the Syafi'I School and the Hanbali School. According to the Shafi'i Madhhab, it is permissible to certify a person who has died, if there is a baby, it is sunnah to be aqiqah. If a parent dies, it is permissible if there is a will from the deceased person. Meanwhile, according to the Hanbali Madzhab, it is permissible to aqiqah a person who has died, be it a baby or an elderly person, as long as the aqiqah is performed in multiples of 7 (a week). And the similarities and differences between the Aqiqah of the Dead According to the Shafi'i and Hanbali schools, the similarities between the two are that they are both an expression of gratitude for the blessings that Allah has given in the form of the birth of a child. When a baby is born, Satan immediately meets him, embraces him, puts him in his hands and tries his best to make him his follower, while the difference between the two is in terms of the law. Meanwhile, in the Hanbali school of law, it is the sunnah of mu'akkad to aqiqah a person who has died. This research is a type of descriptive qualitative research, the method used in this thesis is library research
Keywords: Aqiqah, Madzhab Syafi‟i, Mazhab Hanbali
Study on the Stimulation Effectiveness Evaluation of Large-Scale Hydraulic Fracturing Simulation Experiment Based on Optical Scanning Technology
Authors: Yang Hanzhi , Guo Yintong , Wang Lei , Bi Zhenhui , Guo Wuhao , Zhao Guokai , Yang Chunhe 
Published: April 2022

Summary
Unconventional reservoirs such as gas shales and tight gas sands require technology-based solutions for optimum development because of the undeveloped matrix pores and poor permeability. Hydraulic fracturing is one of the most critical technologies. The quantitative characterization of hydraulic fractures is of great significance to the stimulation evaluation of the reservoir, but there is still a lack of fine, effective and systematic evaluation methods. 3D optical scanning technology is widely used in the quantitative characterization of rock fracture morphology for its advantages of high speed, convenience, high precision, and nondestructive testing. In this study, after the indoor hydraulic-fracturing simulation experiments, 3D optical scanning was used to visualize the fracture network. On this basis, two aspects of quantitative evaluation methods for stimulation effectiveness were established, including: (1) evaluating the local conductivity (permeability) of different fractures by cutting hydraulic-fracturing samples. Then combining local conductivity of different fractures with the overall stimulated reservoir area, which could be more reasonable to evaluate the stimulation scope of the reservoir; (2) calculating the fractal dimension (FD) of the 3D spatial structure based on the point-cloud processing, which could directly reflect the complexity of the fracture network. Finally, a new evaluation index for stimulation (Es) was established to comprehensively assess the stimulation effectiveness of the reservoir, which was applied and verified through the indoor fracturing simulation experiments of tight sandstone from the Ordos Basin, China.
Notch1-CD22-Dependent Immune Dysregulation in the SARS-CoV2-Associated Multisystem Inflammatory Syndrome in Children
Authors: Chatila Talal A. , Benamar Mehdi , Chen Qian , Chou Janet , Julé Amelie , Boudra Rafik , Contini Paola , Crestani Elena , Wang Muyun , Fong Jason , Lai Peggy , Rockwitz Shira , Lee Pui , Chan Tsz Man Fion , Altun Ekin Zeynep , Kepenekli Eda , Karakoc-Aydiner Elif , Ozen Ahmet , Boran Perran , Aygun Fatih , Onal Pinar , Sakalli Ayse Ayzit Kilinc , Cokugras Haluk , Gelmez Metin , Öktelik Fatma , Cetin Esin Aktaş , Zhong Yuelin , Taylor Maria , Irby Katherine , Halasa Natasha , Signa Sara , Prigione Ignazia , Gattorno Marco , Cotugno Nicola , Amodio Donato , Geha Raif , Son Mary Beth , Newburger Jane , Agrawal Pankaj , Volpi Stefano , Palma Paolo , Kiykim Ayca , Randolph Adrienne , Deniz Gunnur , Baris Safa , De Palma Raffaele , Schmitz-Abe Klaus , Charbonnier Louis-Marie , Henderson Lauren 
Published: April 2022

Abstract
Multisystem inflammatory syndrome in children (MIS-C) evolves in some pediatric patients following acute infection with SARS-CoV-2 by hitherto unknown mechanisms. Whereas acute-COVID-19 severity and outcome were previously correlated with Notch4 expression on regulatory T (Treg) cells, here we show that the Treg cells in MIS-C are destabilized in association with increased Notch1 expression. Genetic analysis revealed that MIS-C patients were enriched in rare deleterious variant impacting inflammation and autoimmunity pathways, including dominant negative mutations in the Notch1 regulators NUMB and NUMBL. Notch1 signaling in Treg cells induced CD22, leading to their destabilization in an mTORC1 dependent manner and to the promotion of systemic inflammation. These results establish a Notch1-CD22 signaling axis that disrupts Treg cell function in MIS-C and point to distinct immune checkpoints controlled by individual Treg cell Notch receptors that shape the inflammatory outcome in SARS-CoV-2 infection.
All-optical noise spectroscopy of a solid-state spin
Authors: Farfurnik Demitry , Singh Harjot , Luo Zhouchen , Bracker Allan , Carter Sam , Pettit Robert , Waks Edo 
Published: April 2022

Abstract
Noise spectroscopy elucidates the fundamental noise sources in spin systems, which is essential for developing spin qubits with long coherence times for quantum information processing, communication, and sensing. But noise spectroscopy typically relies on microwave coherent spin control to extract the noise spectrum, which becomes infeasible when there are high-frequency noise components stronger than the available microwave power. Here, we demonstrate an alternative all-optical approach to performing noise spectroscopy. Our approach utilises coherent Raman rotations of the spin state with controlled timing and phase to implement Carr-Purcell-Meiboom-Gill (CPMG) pulse sequences. Analysing the spin dynamics under these sequences enables us to extract the noise spectrum of a dense ensemble of nuclear spins interacting with a single spin in a quantum dot, which has thus far only been modelled theoretically. By providing large spectral bandwidths of over 100 MHz, our Raman-based approach could serve as an important tool to study spin dynamics and decoherence mechanisms for a broad range of solid-state spin qubits.
Some new results on stability and input to state stability of fuzzy differential equations
Authors: GHANMI Boulbaba 
Published: April 2022

Abstract
In this paper, we introduce a new notion of stability, namely generalized practical h −stability. Both generalized practical h −stability and generalized input-to-state practical h −stability are considered. Under Lyapunov techniques, some sufficient conditions are given which guarantee generalized practical h −stability of fuzzy differential equations. The generalized practical h −stability analysis is also accomplished with the help of the scalar h −stable functions.
Effects of supplementation with Shiitake powder, Lentinula edodes, on anti-oxidative activities and energy/ lipid metabolism in healthy dogs
Authors: Kusaba Akio , Okada Yuki , Ueno Hiromichi , Yamamoto Ichiro , Mori Yuichiro , Tanaka Norio , Arai Toshiro , Kawasumi Koh 
Published: April 2022

Abstract
Obesity is the number one health problem pets face. Effective food and supplement should be developed to improve anti-oxidative activities and energy /lipid metabolism in obese animals. In this study, we measured metabolites and hormone concentrations and enzymes activities related to anti-oxidant activities and energy /lipid metabolism in plasma of healthy dogs supplemented with shiitake powder to investigate effects of its supplementation on anti-oxidative function and energy /lipid metabolism in healthy dogs. Shiitake powder was supplemented with healthy dog at the high dose (800mg/kg body weights/day) and low dose (100mg/kg/day) for 4 weeks. In dogs supplemented with shiitake powder at the high dose, plasma SOD activities increased significantly and total cholesterol concentrations decreased and sirtuin1 mRNA expression increased in peripheral leukocytes. Plasma ALP and LDH activities tended to decrease in the high dose group. These reactions seemed to reflect of improvement of energy metabolism and hepatic function. Shiitake powder may ameliorate lipid metabolism in canine liver, and may improve anti-oxidative function and energy/lipid metabolisms in healthy dogs. To evaluate the usefulness of shiitake powder for obese dogs, further studies using overweight and obese dogs are needed. (186 words)