bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroprotective effects of morroniside against Parkinson's Disease via inhibiting oxidative stress and ferroptosis
Authors: Li Mao , Zhang Junli , Jiang Lianyan , Wang Wujun , Feng Xianrong , Liu Meijun , Yang Dongdong 
Published: November 2022

Abstract
Parkinson’s disease (PD) is the second most common neurodegenerative disorder after Alzheimer disease accompanied by the death of dopaminergic neurons and brain nigrostriatal mitochondrial damage in the elderly population. The features of the disease include tremor, rigidity, postural instability, and motor retardation. The pathogenesis of Parkinson’s disease is complex, and abnormal lipid metabolism resulting in ferroptosis due to the excessive accumulation of free radicals from oxidative stress in the substantia nigra of the brain was thought to be one of the factors causing the disease. Morroniside has been reported to have significant neuroprotective effects, although it has not been studied in PD. Therefore, this study focused on determining the neuroprotective effects of morroniside (25, 50, and 100 mg/kg) on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg)-induced mice models of PD and explored 1-methyl-4-phenylpyridinium MPP+-induced ferroptosis in PC12 cells. Morroniside restored the impaired motor function in the PD mice models while reducing neuronal injury. The activation of nuclear factor erythroid 2-related factor 2/antioxidant response elements (Nrf2/ARE) by morroniside promoted antioxidation, the content of reducing agent glutathione (GSH) increased, and the level of the lipid metabolite malondialdehyde (MDA) decreased. Notably, morroniside inhibited ferroptosis in substantia nigra of the brain and PC12 cells, reduced iron levels, and upregulated the expression of the iron-regulated proteins glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11), ferritin heavy chain 1 (FTH-1), and ferroportin (FPN). More importantly, morroniside repaired the mitochondrial damage, restored the mitochondrial respiratory chain, and inhibited the production of reactive oxygen species (ROS). These data indicated that morroniside could activate the Nrf2/ARE signaling pathway to increase the antioxidant capacity, thereby inhibiting abnormal lipid metabolism and protecting dopaminergic neurons from ferroptosis in PD.
Comparatively studies on life cycle, morphometry studies of azuki bean weevil on pigeonpea cultivar ICPL 161 and wild relative accession ICPW 68 in semi-arid tropics
Authors: Kota Sathish , Jaba Jagdish , Mishra Suraj Prashad 
Published: November 2022

Abstract
Legume grains are challenging to store than cereals and infested greatly by insects and microbes. Genus Callosobruchusare one of the most important insects causes both quantitative and qualitative reduction in stored pigeonpea grains. Therefore, the study mainly focused on biological activities of Callosobruchus chinensis on cultivar and wild relative accessions of pigeonpea in the room conditions at International Crops Research Institute for the Semi-Arid Tropics, Telanagana, India during 2018-19 and 2019-20. Test insect culture was obtained from insect rearing cell, ICRISAT. Freshly emerged five pairs of adults were allowed to lay eggs on pigeonpea (ICPL 161 and ICPW 68) grains for 24 hours. The grains containing eggs were collected and stored in vials to record the different stages and morphometrics observations of C. chinensis. The study revealed that ample growth and development of larvae, pupa, adults and highest egg laying capacity of azuki bean weevil were observed on ICPL 161 as compared to ICPW 68. This study will help in development of new Integrated Pest Management (IPM) strategies in combination with host plant resistance, transgenics and pheromones development for management of bruchids.
Factors associated with negative conversion of viral RNA in hospitalized children infected with SARS-CoV-2 Omicron Variant in Shanghai, China:a retrospective analysis
Authors: Yang Yan , You Yannan , Liu Yazun , Geng Lina , Huang Lirong , Zhou Huan , Piao Xiang , Liu Xiao , Wu Mingyun , Wang Yajuan , Zhou Lili , Wang Peng , Shen Shiping , Hu Mingge , Han Zhaopeng , Xue Zheng 
Published: November 2022

Abstract
Objectives: This study aimed to identify the related risk factors and potential predictors of SARS-CoV-2 RNA negative conversion by describing the dynamics of viral shedding in infected children admitted to two hospitals from Shanghai during Omicron variant outbreak.
Methods: This retrospective cohort included laboratory-confirmed cases of SARS-CoV-2 infection from Shanghai between March 28 and May 31, 2022. Clinical characteristics, personal vaccination, household vaccination rates were collected through electronic health records and telephone interviews.
Results: The total of 603 pediatric cases confirmed with COVID-19 was included in this study. Both Univariate and multivariate analysis were performed to filter independent factors for the duration to viral RNA negative conversion. Data on cases re-detected SARS-CoV-2after showing negative results on RT-PCR test (intermittent negative status) were also analyzed. The median duration of virus shedding was 12(Interquartile Qange,IQR: 10-14) days. The severity of clinical outcome, personal vaccination-2doses, household vaccination rates, abnormal defecation were factors indecently affecting negative conversion of SARS-CoV-2 RNA, suggesting that patient who had abnormal defecation or with more severe condition would delay virological clearance, while patient accepted 2 doses vaccination or with higher household vaccination rates would accelerate virological clearance. Loss of appetite (Odds Ratio (OR) :5.343; 95%CI: 3.307-8.632) and abnormal defecation (OR:2.840; 95%CI: 1.736-4.645) were significantly associated with intermittent negative status.
Conclusion: These findings could provide clues for early identification of pediatric patients with prolonged viral shedding, enriching the evidence for development of prevention and control strategies especially the vaccination policies for children and adolescents.
Trends of uterine cancer burden in 204 countries and regions from 2000 to 2019 and disease burden prediction
Authors: Zhao Zhihan , Huang Guotao , He Yong , Zuo Xiaohu , Han Wuyue , Hong Li 
Published: November 2022

Abstract
Background: Uterine cancer is the most common gynecological malignancy and the fourth most common cancer in women. However, the global burden of uterine cancer has not been estimated, and projections of future disease development are lacking.
Methods: Methods: We collected detailed information on the etiology of uterine cancer between 2000 and 2019 from the 2019 Global Burden of Disease Study. Estimated annual percentage changes (EAPCs) of age-standardized incidence rates (ASR) of uterine cancer by region and etiology were calculated to quantify temporal trends in uterine cancer ASR. Predicting future trends in uterine cancer using the EAPC package.
Results: Globally, uterine cancer cases increased from 244,441 in 2000 to 435,041 in 2019, an increase of 78%. During this period, overall ASR increased by an average of 4% per year. The most pronounced increases are usually seen in countries with high sociodemographic indices, such as the United States. The high body-mass index had a significant effect on disability-adjusted life years(DALYs), Deaths, years lived with disability(YLDs), and years of life lost(YLL)s, and 40.2% of DALYs, 39.8% of Deaths, 43.5% of YLDs, and 39.8% of YLLs were attributed to the high body-mass index. EAPC was significantly correlated with ASR (2000) and Human Development Index (HDI) (2019), respectively. Using BAPC models to predict future uterine cancer incidence will continue to rise
Conclusion: The number of uterine cancer cases is on the rise globally. Overall, we observed a higher incidence trend in countries with higher socio-demographic index. High body-mass index is an important risk factor for uterine cancer. This suggests that current prevention strategies should be repositioned and, in some countries, more targeted and specific strategies should be developed to prevent the increase in uterine cancer.
Vitamin D Levels and Left Ventricular Function in Beta Thalassemia Major with Iron Overload
Authors: Pala mrudula , Bhat kamalakshi , Manya Sharath , Joseph Nitin , Harish Sindhu 
Published: November 2022

Abstract
Heart disease is the primary cause of death in patients with Beta Thalassemia Major. The study aimed to determine the association between vitamin D and left ventricular function in patients with Beta-Thalassemia major with iron overload. A cross-sectional hospital-based study was conducted, where the vitamin D and ferritin levels of children living with Beta Thalassemia Major were measured and left ventricular function was assessed utilizing Ejection Fraction (EF) and Fractional Shortening (FS) using 2D Echocardiography. The mean serum ferritin was 4622 ± 2289ng/ml, and the mean serum vitamin D levels were 22 ± 7.7ng/ml. The mean values of EF were 62.30 ± 6.9%, and FS was 31.21 ± 4.8%. Statistically significant negative correlation (r=-0.447, p < 0.001) was found between vitamin D and serum ferritin values, and a significant positive association was found between vitamin D levels concerning EF and FS with a p-value of 0.034 and 0.014, respectively.
DLDH-containing extracellular vesicles from CAFs reduced DOX sensitivity in triple-negative breast cancer
Authors: Xu Zhi , Hou Junchen , Ma Hehua , Zhao Yu , Fei Fei , Sun Runbin , Li Juan 
Published: November 2022

Abstract
Chemo-resistance is a major obstacle in the control of advanced triple-negative breast cancer (TNBC). Cancer-associated fibroblasts (CAFs)-derived extracellular vesicles (EVs) were critical for tumor progression. Herein, we demonstrated that CAFs/TNBC-derived EVs could suppress doxorubicin (DOX) sensitivity in breast cancer both in vitro and in vivo. The protein array revealed that dihydrolipoamide dehydrogenase (DLDH) was enriched in CAFs/TNBC-derived EVs, which was the E3 component of the 2-oxoglutarate dehydrogenase complex (α-KGDC). EVs-DLDH was transported into mitochondria and enhanced mitochondrial respiration through increasing α-KGDC activity and NADH content. Inhibiting DLDH reduced oxidative phosphorylation (OXPHOS) and CAFs-derived EVs-induced drug resistance in the recipient cells. It was also shown that the EVs-reduced sensitivity of DOX was due to increased drug efflux driven by OXPHOS. Additionally, suppression of ATP-binding cassette transporters or mitochondrial respiration conferred the recipient cells with increased susceptibility to DOX. These results elaborated that CAFs-derived EVs inhibit the DOX sensitivity of TNBC through increasing drug efflux driven by DLDH-induced OXPHOS. Inhibiting EVs-DLDH provides a potential therapeutic application to enhance the responsiveness to chemotherapy in TNBC.
Efficacy and tolerability of a specific blend of amino acids in patients with anorexia nervosa treated in a hospital setting. Study protocol for a randomized controlled trial
Authors: Grave Riccardo Dalle , Calugi Simona , Ruocco Chiara , Cimini Marco , Segala Agnese , Ragni Maurizion , Carruba Michele , Valerio Alessandra , Nisoli Enzo 
Published: November 2022

Abstract
Background
Specific blends of essential amino acids (EAA) containing a high percentage of branched-chain amino acids preserves mitochondrial metabolism and higher physical resistance in elderly mice, increasing their survival and improving physical performance and cognitive functions in malnourished elderly patients. However, no study has been yet done on patients with anorexia nervosa (AN) who regain weight with specialized intensive treatment. The present study aims to evaluate the efficacy of supplementation with EAA on the change in lean body mass (LBM) and other physical and psychological outcomes in patients with AN who are undergoing specialist treatment for eating disorders.
Methods
This is a 13-week randomized, double-blind, placebo-controlled study. Patients will be randomized to either a mixture of a complex blend of EAA and intermediates of the tricarboxylic acid (TCA) cycle (citrate, malate, succinate) supplementation (or placebo) upon admission at the intensive residential and day-hospital treatment for eating disorders. Ninety-two participants with AN aged 16–50 years will be recruited from a specialized intensive treatment of eating disorders. Double-blind assessment will be conducted at baseline (T0) and the end of the 13 weeks of treatment (T1). The study's primary aim is to evaluate the efficacy of supplementation with EAA and TCA intermediates on the change in lean body mass (LBM) with weight restoration in patients with AN who are undergoing specialist treatment for eating disorders. The secondary aims of the study are to assess the effect of dietary supplementation on physical fitness, weight restoration, modification of AN and general psychopathology, and psychosocial impairment.
Discussion
The study’s results will inform researchers and clinicians on whether supplementing a mixture of EAA and TCA cycle intermediates will improve the increase of LBM and other important physical and psychological outcomes in patients with AN who regain weight with specialized intensive treatment.
Trial registration: NCT, NCT05290285. Registered 22 March 2022 https://clinicaltrials.gov/ct2/show/NCT05290285
Clinical practice in recurrent pregnancy loss care: identifying possible barriers for the implementation of an evidence-based guideline
Authors: Youssef A. , Lashley E. E.L.O. , Vermeulen N. , Hoorn M. L.P. 
Published: November 2022

Abstract
Background
Practice variation in recurrent pregnancy loss (RPL) care is common. International guidelines vary in their recommendations for the management of RPL couples, which could lead to an increase of cross border reproductive care. Currently, the Dutch RPL guideline is being adapted from the European Society for Human Reproduction and Embryology (ESHRE) guideline. We aim to identify possible barriers in the implementation of evidence-based RPL care as stipulated in the current guidelines. These barriers could be considered in the development of a new guideline to promote adherence to new recommendations.
Methods
A nationwide survey on the management of RPL patients was conducted across all 107 hospital-based obstetrics and gynaecology practices in the Netherlands. The survey was sent via the Dutch Society for Obstetricians and Gynaecologists to all affiliated clinicians. The questionnaire consisted of 36 questions divided in four sections: clinician’s demographics, RPL definition, investigations and therapy. The data were compared to the recommendations given by guideline of the Dutch Society for Obstetricians and Gynaecologists and of the ESHRE, and university hospitals were compared to non-university hospitals.
Results
All hospital-based practices (100%; n=107) filled in the online questionnaire, eight of which were university hospitals, 62 teaching and 37 non-teaching hospitals. The majority of respondents defined RPL similarly, as two or more pregnancy losses (87.4%), not obligatory consecutive (93.1%). Biochemical pregnancies were more often included by university hospitals (57.0% (n=49) vs 41.7% (n=128); p=0.012) by non-university hospitals. More than half of respondents routinely perform thrombophilia screening, although not advised by the ESHRE, while thyroid function, thyroid auto-immunity and β2-glycoprotein antibodies in the context of antiphospholipid syndrome (APS) are recommended but investigated by less than half of respondents. Finally, university hospitals appear to emphasize lifestyle changes more often than non-university hospitals.
Conclusion
While many clinicians perform investigations recommended by the ESHRE, there is a considerable variation of RPL practice in the Netherlands. We identified possible barriers for RPL guideline implementation in the Netherlands, and possibilities for the ESHRE to focus on implementation strategies in collaboration with national societies. This will improve the quality of care provided to RPL patients and may diminish the necessity felt by patients to turn to multiple opinions or cross border reproductive care.
NEXRA: A weather research analysis system based on the Nonhydrostatic Icosahedral Atmospheric Model with a local ensemble transform Kalman filter
Authors: Chen Ying-Wen , Kanemaru Kaya , Kotsuki Shunji , Terasaki Koji , Yashiro Hisashi , Nakano Masuo , Kasami Kyohei , Satoh Masaki , Kubota Takuji , Miyoshi Takemasa 
Published: November 2022

Abstract
This article reviews the first version of the NICAM–LETKF JAXA Research Analysis (NEXRA), a weather research analysis system, which was developed from the fusion of the Nonhydrostatic Icosahedral Atmospheric Model (NICAM) and the local ensemble transform Kalman filter (LETKF) using various observational data, such as NCEP PREPBUFR, radiances from Advanced Microwave Sounding Unit (AMSU)-A, and precipitation obtained from Global Satellite Mapping of Precipitation (GSMaP). The development of NEXRA started in 2017 as a collaboration between the Japan Aerospace Exploration Agency (JAXA), the Atmosphere and Ocean Research Institute at the University of Tokyo (AORI), and the RIKEN Center for Computational Science (R-CCS). NEXRA routinely provides 2-D graphical weather information on the web site for general users and ensemble analysis data for research purposes. Routine operation of the first version of NEXRA started on the JAXA Supercomputer System Generation 2 (JSS2) in June 2018 and ended in February 2021 when JSS2 ceased operation. Routine operation of the second version of NEXRA (NEXRA_2.0) started in April 2021 on the JAXA Supercomputer System Generation 3 (JSS3). This article reviews the development and operation of the first version of NEXRA and describes the use of the ensemble analysis data produced by NEXRA as the atmospheric initial conditions for predictability research in the analysis of three events: the record-breaking rainfall in Japan that occurred in July 2018, Typhoon Krosa in August 2019, and the rapid intensification of Typhoon Haishen in August 2020. Finally, some details of the collaborations between NEXRA and other projects and of the development of the current version of NEXRA (NEXRA_2.0) are given, and a future vision for NEXRA is outlined.
Robust microscale structural superlubricity between graphite and nanostructured surface
Authors: Huang Xuanyu , Li Tengfei , Wang Jin , Xia Kai , Peng Deli , Xiang Xiaojian , Ma Ming , Zheng Quanshui 
Published: November 2022

Abstract
Structural superlubricity (SSL), a state of nearly zero friction and no wear between two contacted solid surfaces, brought a dawn for the revolutionary solution of friction and wear problems. Recently, SSL was realized between microscale graphite flake with two dimensional single-crystalline surface and various non-van der Waals materials, which greatly broadens its application range. However, the SSL state has a certain probability of failure due to the edge defects of graphite flake. Here, we achieve robust SSL state between microscale graphite flakes and nanostructured silicon surfaces under ambient condition. We find that the friction is always less than 1 μN, the differential friction coefficient is on the order of 10-4, without observable wear. Detailed characterization and simulation show that this is attributed to the edge warping of graphite flake on the nanostructured surface under concentrated force, which eliminate the edge interaction between the graphite flake and the substrate. This study proves that a graphite flake with single crystal surface without edge contact with the substrate can universally realize robust SSL state with any non-van der Waals materials in the atmosphere, which reduce the roughness requirements of SSL technology and provides a new method for SSL technology to generally apply in the atmospheric environment.
Selected plant growth promoting rhizobacteria strains for the capability to use orange peel show significant yield increases in soybean grown under field conditions
Authors: Silva Maria Leticia Pacheco Da , Moen Francesco , Liles Mark , Feng Yucheng , Sanz-Saez Alvaro 
Published: November 2022

Abstract
Background and aims.
Plant growth-promoting rhizobacteria (PGPR) can function as biofertilizers to enhance plant growth and crop yield in an environmentally sustainable manner. However, when PGPR are introduced into agricultural soils, their survival is restricted due to limited nutrients available in the rhizosphere. It has been demonstrated that selected Bacillus velezensis (Bv) strains can use pectin-rich orange peel (OP) as a growth substrate, but results have been scarce due to lack of strain screening. Therefore, it is important to select the Bv strains for their capability to utilize OP and test their ability to promote soybean growth when inoculated with OP.
Methods
Six Bv strains were selected by their growth in OP media and then tested for their soybean growth promotion in combination with OP in greenhouse and field experiments.
Results
Among six Bv strains tested in a greenhouse experiment, strains AP191, AP215, and AP216 showed the best results in plant growth promotion when supplemented with OP, where only AP191 with OP significantly enhanced pod dry weight by 15.8%. Inoculation with AP191 + OP showed the greatest yield under field conditions, being the only treatment significantly different from the control treatment (+ 0.5 ton ha− 1, 9.4% yield increase).
Conclusions
These results support the conclusion that soybean seed inoculation with PGPR strains in conjunction with orange peel can produce a yield advantage under field conditions. This research opens the opportunity for more consistent yield increases when using biologics as a seed treatment for the biofertilizer industry.
Chimaeric plant-produced bluetongue virus particles as potential vaccine candidates.
Authors: Gwynn Abigail , Mbewana Sandiswa , Lubisi Alison , Tshabalala Hope , Rybicki Edward , Meyers Ann 
Published: November 2022

Abstract
Bluetongue virus (BTV) causes bluetongue disease in ruminants and sheep. The current live attenuated and inactivated vaccines available for prevention pose several risks, and there is thus a need for vaccines that are safer, economically viable and effective against multiple circulating serotypes. This work describes the development of recombinant virus-like particle (VLP) vaccine candidates in plants which are assembled by co-expression of the 4 BTV serotype 8 major structural proteins. We showed that substitution of a neutralising tip domain of BTV8 VP2 with that of BTV1 VP2 resulted in the assembly of VLPs which stimulated serotype-specific antibodies, as well as virus-specific neutralising antibodies.