bluuebunny/crossref_metadata_2025_split huggingface dataset:
Larvicidal property of the extracts of the seaweeds; Sargassum wightii, S. ilicifolium and Gelidiella acerosa vis-a-vis Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus
Authors: Perumal Pachiappan , Dhanasundaram Sundaramoorthy , Aravinth Annamalai , Amutha Vadivel , Santhanam Perumal 
Published: April 2022

Abstract
Toxicity potential of the 3-solvent extracts of Sargassum wightii, S. ilicifolium and Gelidiella acerosa – seaweeds against the 4th larval-stage of 3 mosquitos was evaluated. The ethyl acetate extracts of the brown seaweeds (S. wightii & S. ilicifolium) and the red seaweed (G. acerosa) exerted the highest larvicidal effect. The S. wightii-ethyl acetate extract (SW-EA) exerted the huge death-rates of Anopheles stephensi with the lethal concentrations of (μg/mL);3.98 - LC50 and 12.17- LC90 followed by 4.43 & 24.44 on Culex quinquefasciatus and 9.26 & 52.00 on Aedes aegypti, respectively. The S. ilicifolium - ethyl acetate (SI-EA) caused the deaths of Anopheles stephensi with the lethal (LC-50&LC-90) doses (μg/mL) of; 18.934 & 371.753, followed by 34.104 & 423.012 on Aedes aegypti, and 40.728 & 683.813 on Culex quinquefasciatus respectively. Corresponding values for the G. acerosa-ethyl acetate-extract (GA-EA) (that caused maximum death-toll of Anopheles stephensi were); 4.59& 15.91, followed by 14.18& 78.77 on Culex quinquefasciatus and 23.261& 354.903 for Aedes aegypti. The algal-analyses showed the common phytochemical - constituents, aliphatic amines, alkynes and alkenes - groups and predominantly the Phytol acetate, OCTADECANOIC ACID and n-Hexadecanoic acid. The generated data form important basis for further investigation towards antimalarial drug development.
Independently Tunable Triple Fano Resonances in Plasmonic Waveguide Structure and Its Applications for Sensing
Authors: Wu Qiaohua , Zhang Yingqiu , Qu Desheng , Li Chunlei 
Published: April 2022

Abstract
A surface plasmon polaritons (SPPs) waveguide structure composed of a metal-insulator-metal (MIM) waveguide with a baffle, a special square cavity (SSC) and a ring cavity (RC), is proposed to realize independent tuning of triple Fano resonances. Using the finite element method (FEM), the magnetic field distributions and optical transmission spectra of the structure are analyzed in detail. The simulation results show that the structure achieves triple Fano resonances originated from two different mechanics. One of Fano resonances occurs in the RC, and the others occur in SSC. By regulating the structural parameters of the SSC and RC, respectively, these Fano resonances can be well tuned independently, which provides flexibility for the structure to be applied to optical devices. In addition, the structure exhibits great performances in refractive index sensing and bio-sensing. The maximum sensitivity of refractive index sensing achieves 2350 nm/RIU, and there is a good linear relationship between resonance wavelength and refractive index. Due to great sensitivity and independent tunability, the SPPs waveguide structure may be potentially used in micro-nano optical devices, especially in optical on-chip sensor.
Bubbles as a feeding predictor improves the welfare of farmed fish
Authors: Kleiber Aude , Le-Calvez Jean-Michel , Kerneis Thierry , Batard Axel , Goardon Lionel , Labbé Laurent , Brunet Valentin , Ferreira Vitor Hugo Bessa , Guesdon Vanessa , Calandreau Ludovic , Colson Violaine 
Published: April 2022

Abstract
Occupational enrichment emerges as a promising strategy for improving the welfare of farmed animals. This form of enrichment aims to stimulate cognitive abilities of animals by providing them with more opportunities to interact with and control their environment. Predictability (i.e., having information about the regularity of salient daily events), and in particular predictability of feeding, is currently one of the most studied occupational enrichment strategies and can take several forms. In fish, while temporal predictability (based on the time of day) of feeding has been widely investigated, signalled predictability (based on a signal, such as light or sound) has received little attention. Depending on the type of predictability used and the ecology of the species, the effects on fish welfare often differ. The present study aimed to determine which feeding predictability would be most appropriate for rainbow trout, the main continental farmed fish in Europe, and what the consequences might be for their welfare. We tested four feeding predictability conditions: temporal (based on time of day), signalled (based on bubble diffusion), temporal + signalled (based on time and bubble diffusion), and unpredictable (random feeding times). Behavioural and zootechnical outcomes recorded were swimming activity, aggressive and stereotypic behaviours, emotional reactivity, and growth. Our results showed that rainbow trout could predict daily feedings relying on time and/or bubbles as predictors as early as two weeks of conditioning. Temporal predictability alone resulted in an increase in pre-feeding aggressive and stereotypic behaviours, suggesting that the use of time as the sole predictor of feedings in husbandry practices may be detrimental to fish welfare. The combination of temporal and signalled predictability elicited the highest conditioned response and the level of pre-feeding aggression and stereotypy tended to be lower than for temporal predictability alone. Signalled predictability with bubbles alone resulted in fewer pre-feeding agonistic and stereotypic behaviours than in the temporal predictability condition. Interestingly, we also found that bubbles were highly attractive regardless of the condition considered. We conclude, therefore, that the use of bubbles as a feeding predictor could represent an interesting approach to improve rainbow trout welfare in farms, by acting as both an occupational and physical enrichment.
CuO-based materials for thermochemical CO2 capture cycles: The influence of the formation of a CuO percolation network on oxygen release and oxidation kinetics
Authors: Imtiaz Qasim , Armutlulu Andac , Donat Felix , Müller Christoph 
Published: April 2022

Abstract
Thermochemical CO2 capture cycles such as chemical looping combustion (CLC) are an economically promising CO2 capture technology that rely on the combustion of a hydrocarbon fuel with lattice oxygen that is derived from a solid oxygen carrier. The oxygen carrier is typically regenerated with air. To increase the agglomeration resistance and redox stability of the oxygen carriers, the active phase is often stabilized with high Tammann temperature ceramics, resulting in the formation of so-called cermet structures. It has been hypothesized that the redox performance of the cermets depends critically on the conduction pathways for solid-state ionic diffusion and the activation energy for charge transport. Here, we investigate the influence of the formation of a percolation network on the electrical conductivity and the rate of oxidation for CeO2-stabilized Cu. We found that for oxygen carriers that contained 60 wt. % CuO, the charge transport occurred predominately via Cu/CuO conduction pathways. Below the percolation threshold of CuO, the conduction of charge carriers took place via CeO2 grains, which formed a continuous network. The measurements of charge transport and redox characteristics confirmed that the activation energy for charge transport through the cermet increased with decreasing Cu content. This indicates that the solid-state diffusion of charge carriers plays an important role during re-oxidation.
An APPSO-SVM approach building the monitoring model of dam safety
Authors: WEN Zhiping , FAN Zhendong , SU Huaizhi 
Published: April 2022

Abstract
The measured data-based dam monitoring model could be used to analyze and predict the dam's operational behavior providing a scientific basis for risk assessment and decision-making of dam management. The traditional dam safety monitoring models established by multiple regression, stepwise regression, time series, gray theory, and other statistical methods have poor robustness and generalization. For this reason, an optimized support vector machine (SVM) whose novelty lies in simple execution procedure, self-adaptive hyperparameter selection from adaptive position particle swarm optimization (APPSO), user-friendly implementation and retention of influence factor combination, is presented for constructing the dam safety monitoring model.
Vibrational and fluorescence spectroscopy mapping structural rearrangement in edible Samia ricini (Eri) pupae proteins during ultrasound assisted extraction and their in-depth proteome profiling
Authors: Sarkar Preeti , Haokip Nemnunhoi , Mahanta Charu Lata , Duary Raj Kumar 
Published: April 2022

Abstract
The present study initially investigated the optimum conditions for maximum protein separation from edible Eri pupae using 150-400W ultrasound power within 5–30 min having a protein yield of > 50% for establishing any significant conformational and physico-chemical alterations. Results showed that 5 min of ultrasonic treatment was feasible to improve extraction yield with increasing power value, best of 250-300W with 20 min treatment. Ultrasound assisted extracted protein at 67.5, 71.4 and 76.4 J mL− 1 exhibited maximum secondary structural changes with an increase in varied disorganized structures. The temperature factor was more evident above 75°C, causing significant shifts in a Fourier transform infrared (FTIR) region of 1620–1642 cm− 1, particularly with a deviation of intermolecular β-sheets. The Raman band intensity pattern at 2929 and 1440–1465 cm− 1 was highest in sonicated treatment at 67.5 J mL− 1 and then significantly declined. Thus, indicating cross-linkage due to the formation of new unstable intermolecular S-S bond in samples treated at 71.4 and 76.4 J mL− 1, which explains the polarity of the microenvironment nearby hydrogen bonds, consistent with particle size and thermal characterization data. The peaks at 2014, 2168, and 2420 cm− 1 gradually disappeared with increased in ultrasonic energy input, probably depicting the development of firm 3-D network-like structures. Around 107 distinct proteins in Eri pupae assigned to 26 insect protein families were deduced via MS/MS investigation of pellet and supernatant. The outcomes will later facilitate in identifying the protein-protein interrelations accountable for functional aspects of product developed with Eri pupae protein isolate.
Geophysical and anthropogenic drivers for global and regional fire emission trends from 2001 to 2019
Authors: Wu Sifeng , Smith Stuart William , Lee Janice Ser Huay 
Published: April 2022

Abstract
Fire plays an important role in the earth system. Carbon emissions from fires affect the global carbon budget and consequently climate change. Biome-specific qualities such as vegetation, climate and human land-use change are altering long-term trends in carbon emissions from fires. Whilst some biomes and continents seem to witness increasing fire-derived carbon emissions, others see decreasing trends. Yet, currently a biome-wide perspective on fire-derived carbon emission trends is lacking. We used total carbon emissions from the Global Fire Emissions Database (version 4 with small fires) to investigate the spatial and temporal dynamics of fire emissions from 2001 to 2019 across the world. While the global trend for fire emissions stayed relatively unchanged (non-significant trend), this was due to contrasting trends in two geographical biomes. Specifically, a decreasing trend in tropical savannas and grasslands, notably across the African continent and an increasing trend in boreal forests, especially a dense cluster in the east Siberian taiga in Boreal Asia. The decrease in emissions in African savannas and grasslands was driven by a decline in vegetation, and thus fuel, and an increase in anthropogenic intervention, especially agricultural expansion. Increase in fire emissions from boreal forests in Boreal Asia was driven by agricultural activities and climate change towards a drier climate (e.g., lower humidity). For many biomes, fire-derived carbon emissions are driven by several anthropogenic activities, vegetation and climate drivers, likely due to the complex feedback governing emissions. This global and biome-wide study highlights that anthropogenic activities in relatively small regions can shape global fire-derived carbon emission trends. Monitoring and management interventions are needed to address increasing fire-derived carbon emission areas, particularly the east Siberian taiga, and other forested biomes where deforestation contributes to rising carbon emission trends.
CFTR, one membrane protein, inhibits proliferation and metastasis of hepatocellular carcinoma through new pathways
Authors: Xu Yijia , Yang Liying , Zhang Mengying , Sun Jianfang , Wen Ruojin , Liu Xin , Lv Kaikai , Wang Qi , Zhao Shangfeng , Teng Ying , Qiu Yingxin , Su Yang , Liu Yanfeng , Zhang Jinghai , Zhao Mingyi 
Published: April 2022

Abstract
Background: CFTR, a chloride channel protein expressed on cell membrane, plays an important role in the transdermal transport of salt and the regulation of intracellular ion concentration, and may be closely related to the occurrence and development of cancer. Methods: In this study, the expression level of CFTR in hepatocellular carcinoma (HCC) and its correlation with metastasis and prognosis were predicted by TGCA, GEPIA and GTEx databases. By constructing MHCC97H with stable overexpression of CFTR and HepG2 with stable interference expression of CFTR cell lines, the expression level of CFTR and its correlation with proliferation and metastasis of HCC cells were preliminarily verified by Western Blot, MTT, Transwell and PI staining. In order to further clarify the potential related mechanism, it was predicted through String and Genecards databases, and the expression level of related proteins was verified by Western Blot. And it was further verified by orthotopic HCC transplanted tumor models combined with in vivo imaging system, western blot and immunohistochemical. Finally, CLUE Command database and autodock was used to predict the potential anti-HCC drugs targeting CFTR.Results: The results showed that the expression of CFTR was significantly lower in human HCC tissues (predicted by clinical data) and HCC cells (HepG2, MHCC97L, MHCC97H), and the expression level was negatively correlated with clinical stage, prognosis (predicted by clinical data) and metastatic potency of HCC cells. CFTR could regulate the metastasis and proliferation of HCC by regulating the expression of MMP2, MMP9, E-cadherin, N-cadherin, and CyclinB1 proteins in vitro and in vivo. CFTR could regulate the expression of HSP90, TERT, and DNAJC6, which meant that HSP90, TERT, and DNAJC6 might build a bridge for CFTR to regulate proliferation and metastasis proteins. Parecoxib might be one potential anti-HCC drug by targeting CFTR.Conclusions: The above studies further clarify a potential new mechanism of HCC occurrence and metastasis, and provide potential new targets and treatment schemes for the diagnosis and treatment of HCC.
Effects of thiostrepton alone or in combination with selumetinib on triple-negative breast cancer metastasis
Authors: KORKMAZ FUNDA DEMIRTAS , Turacli Irem Dogan , ESENDAGLI Guldal , EKMEKCİ Abdullah 
Published: April 2022

Abstract
Objective FoxM1 transcription factor contributes to tumor metastasis and poor prognosis in many cancers including triple-negative breast cancer (TNBC). In this study, we examined the effects of FoxM1 inhibitor Thiostrepton (THIO) alone or in combination with MEK inhibitor Selumetinib (SEL) on metastatic parameters in vitro and in vivo.Methods Cell viability was determined by MTT assay. Immunoblotting and immunohistochemistry was used to assess metastasis-related protein expressions in 4T1 cells and its allograft tumor model in Balb/C mice. In vivo uPA activity was determined by enzymatic methods.Results Both inhibitors were effective on the expressions of FoxM1, ERK, p-ERK, Twist, E-cadherin, and Vimentin alone or in combination in vitro. THIO significantly decreased 4T1 cell migration and changed the cell morphology from mesenchymal-like to epithelial-like structure. THIO was more effective than in combination with SEL in terms of metastatic protein expressions in vivo. THIO alone significantly inhibited mean tumor growth, decreased lung metastasis rate and tumor foci, however, no significant changes in these parameters were observed in the combined group. Immunohistochemically, FoxM1 expression intensity was decreased with THIO and its combination with SEL in the tumors.Conclusion This study suggests that inhibiting FoxM1 as a single target is more effective than combined treatment with MEK in that TNBC allograft model, and targeting FoxM1 via THIO may provide therapeutic benefits in the subset of TNBC tumors with high metastatic capacity.
Explaining the factors affecting women's Life during covid 19: A Qualitative Study among Women in Southern Iran
Authors: Shahabi Nahid , Hosseini Zahra , Homayuni Atefeh , Rad Roghayeh Ezati , Gharibzadeh Abdollah 
Published: April 2022

Abstract
BackgroundThe long-term impact of covid-19 on women's health can cause serious damage to the family and society. In addition to the fear of disease and death, Corona has raised other concerns for women. The present study aims to explain the lockdown experiences among southern Iranian women during Covid-19 pandemic.MethodsIn this qualitative study, which was conducted from November 2020 to March 2021, 20 women who referred to Bandar Abbas health centers were included in the study based on purposive sampling with maximum variation (in terms of age, educational status, marital status, employment). In-depth individual interviews were continued until data saturation, the data were analyzed through conventional content analysis and constant comparison by MAXQDA10 software. ResultsSeven major categories were extracted. they included family-social relations, occupational challenges, financial challenges, leisure time, access to services, psychological reactions, and physical problems. ConclusionThe results showed that lockdown in Covid-19 had a range of positive to negative affecting on women's life. learning new lifestyles and how to live under threat can help manage the negative effects of lockdown.
Crocin Inhibit the Metastasis of MDA-MB-231 cell line by Suppressing Epithelial to Mesenchymal Transition through WNT/β-catenin Signaling Pathway
Authors: Dariushnejad Hassan , ALJAF Karwan Anwar Hassan , Wasman Hunar Mustafa , Pirzeh Lale , Ghorbanzadeh Vajihe 
Published: April 2022

Abstract
Breast cancer is divided into different subtypes based on molecular characteristics, among these subtypes, Triple-negative breast cancer, has the poorest prognosis and survival with invasive. In this study, TNBC cell line was used to explore crocin anti-metastatic effect on the Wnt/β-catenin pathway. Cell proliferation assessed by MTT assay and effects of crocin on migration monitored by transwell and wound healing experiments. Expression of certain epithelial-mesenchymal transition (EMT) markers genes was evaluated by real-time PCR. β-catenin expression also examined by real-time PCR. Findings revealed crocin significantly inhibits cell proliferation and migration of tumor cells in a dose-dependent manner. Moreover, crocin decreased the expression of Vimentin, Snail, Zeb-1 and β-catenin. Also, crocin increased the expression of E-cadherin in MDA-MB-231 cell line. Results showed an association between crocin and Wnt/β-catenin signaling pathway. In conclusion, this study establishes that crocin can be a promising therapeutic for triple-negative breast cancer.
Association of xenobiotic-metabolizing genes polymorphisms with cervical cancer risk in the Tunisian population
Authors: helaoui ahlem , Sfar Sana , Boudhiba Najet , Dehghanian Fariba , Dehbashi Moein , Bouchahda Haifa , Hojati Zohra , KENANI Abderraouf 
Published: April 2022

Abstract
Background: Host genetic characteristics and environmental factors interactions may play a crucial role in cervical carcinogenesis. We investigated the impact of functional genetic variants of four xenobiotic-metabolizing genes (AhR, CYP1A1, GSTM1, and GSTT1) on cervical cancer development in Tunisian women. Methods: The AhR gene polymorphism was analyzed using the tetra-primer ARMS-PCR, whereas the CYP1A1 polymorphism genotypes were identified by PCR-RFLP. A multiplex ligation-dependent polymerase chain reaction approach was applied for the analysis of GSTM1 and GSTT1 polymorphisms. Results: The homozygous A/A genotype of the AhR gene (rs2066853) and the heterozygous T/C genotype of the CYP1A1 SNP (CYP1A1-MspI) appeared to be associated with an increased risk of cervical tumorigenesis (ORa = 2.81; ORa = 5.52, respectively). Furthermore, a significantly increased risk of cervical cancer was associated with the GSTT1 null genotype (ORa = 2.65). However, the null GSTM1 genotype showed any significant association with the risk of cervical cancer compared to the wild genotype (ORa = 1.18; p = 0.784). Considering the combined effect, we noted a significantly higher association with cancer risk for individuals with at least two high-risk genotypes of CYP1A1/GSTT1 (ORa = 4.2), individuals with at least two high-risk genotypes of CYP1A1/GSTT1/AhR (ORa = 11.3) and individuals with at least two high-risk genotypes of CYP1A1/GSTM1/GSTT1/AhR exploitation low-risk genotype as a reference. Conclusion: This study indicated that the single-gene contribution and the combined effect of xenobioticmetabolizing gene polymorphisms (AhR, CYP1A1-MspI, GSTM1, and GSTT1) may have a considerable association with increased cervical cancer risk.
Identification of molecular subtypes of chronic obstructive pulmonary disease by gene expression profiling
Authors: zhangpingan 张平安 , Gao Na , Li Xiaoning , Ji Guochao , Wu Jianjun 
Published: April 2022

Abstract
BackgroundChronic obstructive pulmonary disease (COPD) has become the fourth most lethal disease in the world and is expected to rise to the third most lethal disease in the world after 2030.COPD is complex and has clinical heterogeneity. However, identifying the subgroup characteristics of chronic obstructive pulmonary disease has become a challenge.ObjectivesThe ultimate goal of studying these different subgroups is to find patients with unique treatment goals and formulate targeted treatment plans to improve the prognosis of the disease and improve the quality of life of patients.MethodsWe obtained the relevant gene chip by searching the gene expression omnibus (GEO) database. According to the gene expression profile, 151patients with chronic obstructive pulmonary disease were divided into three subgroups, which showed different expression patterns. Therefore, we used weighted gene coexpression analysis (WGCNA) to identify the differences between different subgroups and identified five subgroup specific weighted gene coexpression analysis modules.ResultsThe characteristics of WGCNA module showed that subjects in subgroup I showed airway remodeling characteristics; Subjects in subgroup II showed metabolic activity; Subjects in subgroup III showed inflammatory characteristics.ConclusionsThis study obtained the clinical subgroup classification of chronic obstructive pulmonary disease through consensus clustering, and found that patients in different subgroups may have unique gene expression patterns, which can help researchers explore new treatment strategies for COPD according to the characteristics of clinical subgroups.