bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Cobalt–Chromium–Molybdenum Implant Surface Modifications on Biofilm Development of S. aureus and S. epidermidis
Authors: Paulitsch-Fuchs Astrid H. , Bödendorfer Benjamin , Wolrab Lukas , Eck Nicole , Dyer Nigel P. , Lohberger Birgit 
Published: March 2022

Periprosthetic infections are an eminent factor in patient care and also having significant economic implications. The number of biofilm-infection related replacement surgeries is increasing and will continue to do so in the following decades. To reduce both the health burden of the patients and the costs to the healthcare sector, new solutions for implant materials resistant to such infections are necessary. This study researches different surface modifications of cobalt–chromium–molybdenum (CoCrMo) based implant materials and their influence on the development of biofilms. Three smooth surfaces (CoCrMo, CoCrMo TiN, and CoCrMo polished) and three rough surfaces (CoCrMo porous coated, CoCrMo cpTi, and CoCrMo TCP) are compared. The most common infectious agents in periprosthetic infections are Staphylococcus aureus and Coagulase-negative staphylococci (e.g., Staphylococcus epidermidis), therefore strains of these two species have been chosen as model organisms. Biofilms were grown on material disks for 48 h and cell number, polysaccharide content, and protein contend of the biofilms were measured. Additionally, regulation of genes involved in early biofilm development (S. aureus icaA, icaC, fnbA, fnbB, clfB, atl; S. epidermidis atlE, aap) was detected using RT-q-PCR. All results were compared to the base alloy without modifications. The results show a correlation between the surface roughness and the protein and polysaccharide content of biofilm structures and also the gene expression of the biofilms grown on the different surface modifications. This is supported by the significantly different protein and polysaccharide contents of the biofilms associated with rough and smooth surface types. Additionally, early phase biofilm genes (particularly icaA, icaC, and aap) are statistically significantly downregulated compared to the control at 48 h on rough surfaces. CoCrMo TiN and polished CoCrMo were the two smooth surface modifications which performed best on the basis of low biofilm content.
Potent Antibacterial and Antibiofilm Activities of a Synthetic Remineralizing Preparation of Nano-Hydroxyapatite Against Cariogenic Streptococcus mutans Using an Ex-vivo Animal Model
Authors: El-Gar Yasmeen H. Abo , Etman Wedad M. , Genaid Thuraia M. , Al-Madboly Lamiaa A. 
Published: March 2022

AimThe aim to this study is to evaluate the biocompatibility and antibiofilm actions of two nano-hydroxy apatite (NHA).MethodologyNano-hydroxy apatites are biomaterials use in direct contact with living tissues. Therefore, they should be tested for their safety beside assessment of their minimum inhibitory (MIC) and minimum bactericidal concentration (MBC) using broth microdilution method. One hundred and twenty extracted bovine incisors were collected and cleaned to ensure the absence of any defects. Enamel blocks with different size (2 × 2 × 3 mm) and (5 × 5 × 2 mm) were prepared from their labial surfaces using an isomet saw. Enamel blocks are used for detecting the suitable concentration will be used in the following experiments using energy dispersive X-ray analysis (EDX). The remaining enamel blocks divided into 5 equal groups to detect inhibitory effect against bacterial adhesion to the initial enamel caries like lesions using viable count technique beside the antibiofilm activity against mature biofilm of Streptococcus mutans (S. mutans) using confocal laser microscopy. The remaining enamel blocks were used as a representing data for detecting surface topography for each group by using the scanning electron microscopy (SEM).ResultThe data showed safety of NHA suspensions. Additionally, only NHA suspension of large nanoparticle size (NHA-LPS) had MIC of 1.25 mg/ml against S. mutans. Also, have the higher percentages of Ca and P in the enamel blocks. Furthermore, the lowest level of bacterial adhesion was recorded in (group III) treated by NHA-LPS which was non-significantly different with the positive control group V. Biofilm thickness in group IV treated with NHA-small particle size (SPS) recorded high biofilm thickness followed by group III. Interestingly, group III showed greater killing effect against mature biofilm which is slightly higher than the positive control group V. In group III, surface topography revealed very smooth enamel surface with closed pores. Accordingly, NHA-LPS suspension had antiadhesive, antibacterial, and antibiofilm effect against cariogenic S. mutans representing a promising possibility to be recommended for safe effective remineralization.
Chromosome-Scale Assembly of the Dendrobium nobile Genome Provides Insights Into the Molecular Mechanism of the Biosynthesis of the Medicinal Active Ingredient of Dendrobium
Authors: Xu Qing , Niu Shan-Ce , Li Kang-Li , Zheng Pei-Ji , Zhang Xiao-Jing , Jia Yin , Liu Yang , Niu Yun-Xia , Yu Li-Hong , Chen Duan-Fen , Zhang Guo-Qiang 
Published: March 2022

Orchids constitute approximately 10% of flowering plant species. However, only about 10 orchid genomes have been published. Metabolites are the main way through which orchids respond to their environment. Dendrobium nobile, belonging to Dendrobium, the second largest genus in Orchidaceae, has high ornamental, medicinal, and ecological value. D. nobile is the source of many popular horticultural varieties. Among the Dendrobium species, D. nobile has the highest amount of dendrobine, which is regarded as one of the criteria for evaluating medicinal quality. Due to lack of data and analysis at the genomic level, the biosynthesis pathways of dendrobine and other related medicinal ingredients in D. nobile are unknown. In this paper, we report a chromosome-scale reference genome of D. nobile to facilitate the investigation of its genomic characteristics for comparison with other Dendrobium species. The assembled genome size of D. nobile was 1.19 Gb. Of the sequences, 99.45% were anchored to 19 chromosomes. Furthermore, we identified differences in gene number and gene expression patterns compared with two other Dendrobium species by integrating whole-genome sequencing and transcriptomic analysis [e.g., genes in the polysaccharide biosynthesis pathway and upstream of the alkaloid (dendrobine) biosynthesis pathway]. Differences in the TPS and CYP450 gene families were also found among orchid species. All the above differences might contribute to the species-specific medicinal ingredient biosynthesis pathways. The metabolic pathway-related analysis will provide further insight into orchid responses to the environment. Additionally, the reference genome will provide important insights for further molecular elucidation of the medicinal active ingredients of Dendrobium and enhance the understanding of orchid evolution.
Development and Validation of a Ferroptosis-Related lncRNAs Prognosis Model in Oral Squamous Cell Carcinoma
Authors: Li Tao , Wang Yi , Xiang Xianwang , Chen Chuanjun 
Published: March 2022

Objectives: Ferroptosis is an iron-dependent form of programmed cell death, which affects the prognosis of many cancers. Some long non-coding RNA (lncRNA) can affect the prognosis of cancer by regulating the process of ferroptosis. However, the role of ferroptosis-related lncRNA (frlncRNA) in oral squamous cell carcinoma (OSCC) is not yet clear.Materials and Methods: The data of OSCC patients were downed from The Cancer Genome Atlas (TCGA). After univariate and multivariate Cox regression analysis, the prognosis-related ferroptosis-related lncRNAs were obtained to construct a prognostic model. Calculated the risk score to divide patients into high and low risk groups, and evaluated the predictive ability of the model and the differential expression of immunity in the high and low risk groups.Results: The prognostic model for OSCC was constructed based on 8 prognostic-related frlncRNAs which co-expressed with 25 mRNAs. Kaplan-Meier analyses displayed that the risk score is inversely proportional to patient survival. Receiver operating characteristic (ROC) and decision curve analysis (DCA) indicated that the risk score is superior to other clinical characteristics, and independent prognostic analysis demonstated that risk score is independent factor for the overall survival (OS) rate. The results of immunological analysis showed differences in immune cells, functions, immune checkpoints, and m6A expression between high and low risk groups.Conclusion: We constructed an OSCC patients prognosis model based on 8 frlncRNAs, which can provide prognostic evaluation and immune analysis for OSCC patients, and provided new direction for OSCC targeted therapy.
Bottom-Up and Top-Down Attention Impairment Induced by Long-Term Exposure to Noise in the Absence of Threshold Shifts
Authors: Wang Ying , Huang Xuan , Zhang Jiajia , Huang Shujian , Wang Jiping , Feng Yanmei , Jiang Zhuang , Wang Hui , Yin Shankai 
Published: March 2022

ObjectiveWe aimed to assess the effect of noise exposure on bottom-up and top-down attention functions in industrial workers based on behavioral and brain responses recorded by the multichannel electroencephalogram (EEG).MethodIn this cross-sectional study, 563 shipyard noise-exposed workers with clinical normal hearing were recruited for cognitive testing. Personal cumulative noise exposure (CNE) was calculated with the long-term equivalent noise level and employment duration. The performance of cognitive tests was compared between the high CNE group (H-CNE, &gt;92.2) and the low CNE group; additionally, brain responses were recorded with a 256-channel EEG from a subgroup of 20 noise-exposed (NG) workers, who were selected from the cohort with a pure tone threshold &lt;25 dB HL from 0.25 to 16 kHz and 20 healthy controls matched for age, sex, and education. P300 and mismatch negativity (MMN) evoked by auditory stimuli were obtained to evaluate the top-down and bottom-up attention functions. The sources of P300 and MMN were investigated using GeoSource.ResultsThe total score of the cognitive test (24.55 ± 3.71 vs. 25.32 ± 2.62, p &lt; 0.01) and the subscale of attention score (5.43 ± 1.02 vs. 5.62 ± 0.67, p &lt; 0.001) were significantly lower in the H-CNE group than in the L-CNE group. The attention score has the fastest decline of all the cognitive domain dimensions (slope = −0.03 in individuals under 40 years old, p &lt; 0.001; slope = −0.06 in individuals older than 40 years old, p &lt; 0.001). When NG was compared with controls, the P300 amplitude was significantly decreased in NG at Cz (3.9 ± 2.1 vs. 6.7 ± 2.3 μV, p &lt; 0.001). In addition, the latency of P300 (390.7 ± 12.1 vs. 369.4 ± 7.5 ms, p &lt; 0.001) and MMN (172.8 ± 15.5 vs. 157.8 ± 10.5 ms, p &lt; 0.01) was significantly prolonged in NG compared with controls. The source for MMN for controls was in the left BA11, whereas the noise exposure group's source was lateralized to the BA20.ConclusionLong-term exposure to noise deteriorated the bottom-up and top-down attention functions even in the absence of threshold shifts, as evidenced by behavioral and brain responses.
Preoperative Prediction Power of Radiomics for Breast Cancer: A Systemic Review and Meta-Analysis
Authors: Li Zhenkai , Ye Juan , Du Hongdi , Cao Ying , Wang Ying , Liu Desen , Zhu Feng , Shen Hailin 
Published: March 2022

BackgroundTo evaluate the preoperative predictive value of radiomics in the diagnosis of breast cancer (BC).MethodsBy searching PubMed and Embase libraries, our study identified 19 eligible studies. We conducted a meta-analysis to assess the differential value in the preoperative assessment of BC using radiomics methods.ResultsNineteen radiomics studies focusing on the diagnostic efficacy of BC and involving 5865 patients were enrolled. The integrated sensitivity and specificity were 0.84 (95% CI: 0.80–0.87, I2 = 76.44%) and 0.83 (95% CI: 0.78–0.87, I2 = 81.79%), respectively. The AUC based on the SROC curve was 0.91, indicating a high diagnostic value.ConclusionRadiomics has shown excellent diagnostic performance in the preoperative prediction of BC and is expected to be a promising method in clinical practice.
Systematic Review and Meta-analysis: Association of Aspirin With Incidence of Hepatocellular Carcinoma
Authors: Zhou Xueliang , Zhang Tengfei , Sun Yali , Li Chunwei , Ding Xianfei , Zhu Yanhui , Li Lifeng , Fan Zhirui 
Published: March 2022

Aim: To explore the relationship between the use of aspirin and the incidence of hepatocellular carcinoma (HCC).Methods: MEDLINE, EMBASE, Web of Science and Cochrane CENTRAL databases were searched systematically from the earliest available date to 13 March 2020. The primary outcome was incidence of HCC, and the secondary outcomes were recurrence and mortality of HCC. The results were expressed as the Hazard Ratio (HR) and 95% confidence interval (CI). Based on the heterogeneity evaluated with the I2 statistic, a meta-analysis was performed using either a random- or fixed-effects model.Results: A total of sixteen articles (2781100 participants) were included. There was lower incidence of HCC in aspirin users than those in non-aspirin users (HR, 0.56; 95% CI, 0.46-0.69; p &lt; 0.001). Subgroup analysis further showed that the incidence of liver cancer in patients with alcoholic cirrhosis (HR, 0.14; 95% CI, 0.09-0.22; p &lt; 0.001) and virus hepatitis (HR, 0.68; 95% CI, 0.62-0.74; p &lt; 0.001) who use aspirin was lower than that of patients who do not use aspirin. In addition, aspirin was found to associate with decreased risk of HCC mortality (HR, 0.71; 95% CI, 0.65-0.78; p &lt; 0.001), not HCC recurrence (HR, 0.52; 95% CI, 0.15-1.76; p = 0.291).Conclusions: Aspirin use is significantly associated with the low incidence rate of liver cancer.
Site- and Time-Dependent Compositional Shifts in Oral Microbiota Communities
Authors: Esberg Anders , Eriksson Linda , Johansson Ingegerd 
Published: March 2022

ObjectivesThe oral microbiota plays a significant role in oral health. The present study aims to characterize variations in the oral microbiota relative to the collection site, the dynamics of biofilm accumulation, and inherent inter-individual differences.MethodsWhole stimulated saliva and tooth biofilm samples from the 16 defined tooth regions were collected after 1, 2, or 3 days without oral hygiene (accumulation time) in six healthy adults with no signs of active caries or periodontal disease. The routines and conditions before and between sample collections were carefully standardized. Genomic DNA was extracted, and the V3-V4 regions of the 16S rRNA gene were amplified by PCR and sequenced on an Illumina MiSeq platform. Sequences were quality controlled, amplicon sequence variants (ASVs) were clustered, and taxonomic allocation was performed against the expanded Human Oral Microbiome Database (eHOMD). Microbial community profiles were analyzed by multivariate modeling and a linear discriminant analysis (LDA) effect size (LEfSe) method.ResultsThe overall species profile in saliva and tooth biofilm differed between participants, as well as sample type, with a significantly higher diversity in tooth biofilm samples than saliva. On average, 45% of the detected species were shared between the two sample types. The microbiota profile changed from the most anterior to the most posterior tooth regions regardless of whether sampling was done after 1, 2, or 3 days without oral hygiene. Increasing accumulation time led to higher numbers of detected species in both the saliva and region-specific tooth biofilm niches.ConclusionThe present study confirms that the differences between individuals dominate over sample type and the time abstaining from oral hygiene for oral microbiota shaping. Therefore, a standardized accumulation time may be less important for some research questions aiming at separating individuals. Furthermore, the amount of DNA is sufficient if at least two teeth are sampled for microbiota characterization, which allows a site-specific characterization of, for example, caries or periodontitis.
EXPLORATORY FACTOR ANALYSIS (EFA) OF PERFORMANCE OF SMES
Authors: - Dede Ansyari Guci 
Published: March 2022

Performance of SMEs in Indonesia has been a lot of research on previous study. The objective of this research is to develop an idea for calculate performance of SMEs. As many as 100 respondents of pilot test of SME players in Medan, Indonesia. The validation method aimed at validating the parameters which calculate each of the constructs by statitiscally determining that the sample is adequate, using KMO and barlett’s test to confirm the effectiveness of the data for multivariate statistical analysis, validating the calculation requirements as applicable to performance of SMEs adept. The result show that the data is valid and reliable design.
När begäret går överstyr:
Authors: Dziedzic Dominik 
Published: March 2022

I denna artikel prövas en läsning av Viktor Rydbergs Singoalla ur ett skevperspektiv. Fokus sätts på barnkonstruktionen i texten. Singoalla presenteras som en hybrid- roman med skräckromantiska inslag, vilket påverkar hur karaktären Sorgbarn, sonen till huvudprotagonisterna Singoalla och Erland, konstrueras. Genom att det skräckromantiska lyfts fram diskuteras Sorgbarn som en skev gestalt som avviker från den idealistiska norm utifrån vilken barnframställningen i Rydbergs litterära produktion annars konstrueras. Med Sorgbarns skevhet avses att han,
till skillnad från de idealiserade barngestalterna i Rydbergs övriga författarskap, förmedlar och genererar begär som inte låter sig harmoniskt integreras i hand- lingen. Gossens skevhet diskuteras i relation till det kusliga, det monstruösa och queera begär. Sorgbarns blotta närvaro i sin far Erlands slott leder till ett förfall av Erlands psyke, vilket i denna artikel diskuteras genom Freuds husmetafor. Romanen läses som en text som handlar om att förlora kontrollen över begär, förmedlade och genererade genom pojkkroppen.
Anticoagulant treatment and COVID-19
Authors: ÖZDEMIR İbrahim Halil , ÖZEN Mehmet Burak , GÜNDÜZ Ramazan 
Published: March 2022

Objective: In this study, we aimed to examine the effect of atrial fibrillation (AF) on mortality in COVID-19 patients and the risk scores that can be used in determining risky AF patients. In addition, we aimed to examine the place of oral anticoagulant use in treatment.
Materials and Methods: Records of patients diagnosed with COVID-19 were retrospectively reviewed. Among these patients, 48 patients with a diagnosis of AF were identified. Intensive care need, mortality, CHA2DS2VASC and HAS-BLED scores of the patients were recorded. CRP and D-Dimer levels were recorded. Oral anticoagulant treatments used by the patients were recorded.
Results: The CHA2DS2VASC (intensive care: 4,50±1,41, internal medicine service: 2,50±1,05, p
Prognostic Value of Hematological Parameters in Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis
Authors: SOYSAL GÜNDÜZ Özgül , TORAMAN Aysun 
Published: March 2022

Objective: Hematological parameters including neutrophil–lymphocyte ratio (NLR), platelet–lymphocyte ratio (PLR), and monocyte–lymphocyte ratio (MLR) have been demonstrated as new predictors of prognosis and activity in various diseases. In this study, we aimed to investigate the associations of NLR, PLR and MLR with disease severity and prognosis in patients with anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis (AAV).
Materials and Methods: Medical records of 92 AAV patients were investigated retrospectively. The clinical and laboratory data, and disease outcomes were recorded. The patients having five factor score (FFS) ≥ 2 were categorized as severe disease.
Results: 92 patients with a median age of 60 years were included. Patients with NLR≥4.8 (RR 1.83), PLR≥151.8 (RR 2.02) and MLR≥0.38 (RR 1.85) had higher risk of severe disease. In correlation analysis, NLR, PLR and MLR were positively correlated with C reactive protein and erythrocyte sedimentation rate (ESR). NLR, PLR and MLR were associated with remission.
Conclusion: Only the patients with high PLR had higher relapse rate. MLR was also correlated with development of end-stage renal disease (ESRD). Renal involvement was more frequent in patients with high levels of NLR, PLR and MLR.
Conclusions: We showed that NLR, PLR and MLR at diagnosis were associated with the severity and prognosis of AAV. This is the first study, showing the correlation of MLR with disease severity and ESRD in patients with AAV.
Yenidoğanlarda kritik konjenital kalp hastalığı tarama sonuçlarımız
Authors: TANRIVERDİ Sema , ATİK Sinem , ŞENOL Coşkun 
Published: March 2022

Giriş ve Amaç: Konjenital kalp hastalığı (KKH), en sık görülen konjenital hastalıktır. Kritik konjenital kalp hastalığı (KKKH) ise, yaşamın ilk 28 gününde cerrahi operasyon ya da kateter girişimi gereksinimi olan KKH’dır. Doğumdan sonra KKKH tanısı geciken vakalarda mortalite ve morbidite artar. Bu çalışmada hastanemizde taburculuk öncesi uygulanan nabız oksimetre ile KKKH taraması sonuçlarının değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntemler: Ocak 2019 ile Ocak 2020 tarihleri arasında hastanemizde doğan ve anne yanında takip edilen 504 yenidoğan bebek çalışmaya dahil edildi. Prenatal dönemde KKKH tanısı almış ya da KKH dışı major anomalisi olan bebekler çalışmaya dahil edilmedi. Taburculuk öncesi nabız oksimetre ile preduktal ve postduktal saturasyon ölçümleri yapıldı.
Bulgular: Çalışmaya toplam 504 bebek alındı. Bebeklerin ortalama doğum haftası 38,27 ± 1,35 (35-42) hafta, doğum ağırlığı ise 3200,29 ± 476,05 (1980-4970) olarak saptandı. Kız/erkek oranı 250/254; sezeryan/normal doğum oranı 337/167 idi. Tarama sırasındaki bebeklerin ortalama yaşları 32,51 ± 17,87 (6-96) saatti. Tarama sonucu ortalama kardiak nabız 140,06 ± 15,03 (99-186) /dk, preduktal saturasyon değeri %97,66 ± 1,92 (89-100), postduktal saturasyon değeri %97,88 ± 1,84 (90-100), fark %1,49 ± 1,40 (0-7) olarak saptandı. İlk taramadan 85 (%16,9) bebek kalırken, ikinci tarama yapılan 85 bebeğin 56 (%11,1)’sı kaldı. Tarama ve fizik muayene sonrası EKO’su yapılan 72 bebeğin 45inde patoloji saptanırken 27’sinde normal olarak değerlendirildi. Hiç bir hastada KKKH saptanmadı.
Sonuç: Birçok merkezde de yapılan nabız oksimetre ile KKKH tarama sonuçlarımızı değerlendirdik. Yapılan değerlendirmede tarama testinden kalan hastaların hiçbirinde KKKH saptanmadı. Bunun da düzenli perinatal takip ve ayrıntılı ultrasonografik değerlendirmeye bağlı olduğu düşünüldü.