bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of separated pair housing of female C57BL/6JRj mice on well-being
Authors: Hohlbaum K. , Merle R. , Frahm S. , Rex A. , Palme R. , Thöne-Reineke C. , Ullmann K. 
Published: May 2022

AbstractIn laboratory animal facilities, it is a common code of practice to house female mice in groups. However, some experimental conditions require to house them individually, even though social isolation may impair their well-being. Therefore, we introduced a separated pair housing system and investigated whether it can refine single housing of adult female C57BL/6JRj mice. Individually ventilated cages (IVC) were divided by perforated transparent walls to separate two mice within a cage. The cage divider allowed visual, acoustic, and olfactory contact between the mice but prevented interindividual body-contact or food sharing. Short- and long-term effects of the separated pair housing system on the well-being of the mice were compared with single and group housing using a range of behavioral and physiological parameters: Nest building behavior was assessed based on the complexity of nests, the burrowing performance was measured by the amount of food pellets removed from a bottle, and trait anxiety-related behavior was tested in the free exploratory paradigm. For the evaluation of the ease of handling, interaction with the experimenter's hand was monitored. Social interaction with unknown conspecifics and locomotor activity were investigated in a test arena. Moreover, body weight and stress hormone (metabolites) were measured in feces and hair. After the mice spent a day under the respective housing conditions, concentrations of fecal corticosterone metabolites were higher in separated pair-housed mice, and they built nests of a higher complexity when compared to single-housed mice. The latter effect was still observable eight weeks later. In week 8, separated pair-housed mice showed less locomotor activity in the social interaction arena compared to mice from the other housing systems, i.e., single and group housing. Regardless of the time of testing, pair housing improved the burrowing performance. Separated pair-housed mice were more difficult to catch than group-housed mice. Hair corticosterone, progesterone, and dehydroepiandrosterone concentrations changed with increasing age independently of the housing system. There were no effects of the housing systems on trait anxiety-related behavior in the free exploratory paradigm, voluntary interaction with the experimenter’s hand, and body weight. Overall, the transfer to the separated pair housing system caused short-term stress responses in female C57BL/6JRj mice. Long-term effects of separated pair housing were ambiguous. On one hand, separated pair housing increased nesting and burrowing behavior and may therefore be beneficial compared to single housing. But on the other hand, locomotor activity decreased. The study underlined that the effects of the housing conditions on physiological and behavioral parameters should be considered when analyzing and reporting animal experiments.
The role of polymeric chains as a protective environment for improving the stability and efficiency of fluorogenic peptide substrates
Authors: Arnaiz Ana , Guembe-García Marta , Delgado-Pinar Estefanía , Valente Artur J. M. , Ibeas Saturnino , García José M. , Vallejos Saúl 
Published: May 2022

AbstractWe have faced the preparation of fully water-soluble fluorescent peptide substrate with long-term environmental stability (in solution more than 35 weeks) and, accordingly, with stable results in the use of this probe in determining the activity of enzymes. We have achieved this goal by preparing a co-polymer of the commercial N-vinyl-2-pyrrolidone (99.5% mol) and a fluorescent substrate for trypsin activity determination having a vinylic group (0.5%). The activity of trypsin has been measured in water solutions of this polymer over time, contrasted against the activity of both the commercial substrate Z-L-Arg-7-amido-4-methylcoumarin hydrochloride and its monomeric derivative, prepared ad-hoc. Initially, the activity of the sensory polymer was 74.53 ± 1.72 nmol/min/mg of enzyme, while that of the commercial substrate was 20.44 ± 0.65 nmol/min/mg of enzyme, the former maintained stable along weeks and the latter with a deep decay to zero in three weeks. The ‘protection’ effect exerted by the polymer chain has been studied by solvation studies by UV–Vis spectroscopy, steady-state & time resolved fluorescence, thermogravimetry and isothermal titration calorimetry.
A comprehensive evaluation of COVID-19 policies and outcomes in 50 countries and territories
Authors: Tsou Hsiao-Hui , Kuo Shu-Chen , Lin Yu-Hsuan , Hsiung Chao A. , Chiou Hung-Yi , Chen Wei J. , Wu Shiow-Ing , Sytwu Huey-Kang , Chen Pau-Chung , Wu Meng-Hsuan , Hsu Ya-Ting , Wu Hsiao-Yu , Lee Fang-Jing , Shih Shu-Man , Liu Ding-Ping , Chang Shan-Chwen 
Published: May 2022

AbstractThe COVID-19 pandemic struck the world unguarded, some places outperformed others in COVID-19 containment. This longitudinal study considered a comparative evaluation of COVID-19 containment across 50 distinctly governed regions between March 2020 and November 2021. Our analysis distinguishes between a pre-vaccine phase (March–November 2020) and a vaccinating phase (December 2020–November 2021). In the first phase, we develop an indicator, termed lockdown efficiency (LE), to estimate the efficacy of measures against monthly case numbers. Nine other indicators were considered, including vaccine-related indicators in the second phase. Linear mixed models are used to explore the relationship between each government policy & hygiene education (GP&HE) indicator and each vital health & socioeconomic (VH&SE) measure. Our ranking shows that surveyed countries in Oceania and Asian outperformed countries in other regions for pandemic containment prior to vaccine development. Their success appears to be associated with non-pharmaceutical interventions, acting early, and adjusting policies as needed. After vaccines have been distributed, maintaining non-pharmacological intervention is the best way to achieve protection from variant viral strains, breakthrough infections, waning vaccine efficacy, and vaccine hesitancy limiting of herd immunity. The findings of the study provide insights into the effectiveness of emerging infectious disease containment policies worldwide.
Establishment of apiary-level risk of American foulbrood through the detection of Paenibacillus larvae spores in pooled, extracted honey in Saskatchewan
Authors: Zabrodski Michael W. , Epp Tasha , Wilson Geoff , Moshynskyy Igor , Sharafi Mohsen , Reitsma Lara , Castano Ospina Mateo , DeBruyne Jessica E. , Wentzell Alexandra , Wood Sarah C. , Kozii Ivanna V. , Klein Colby D. , Thebeau Jenna , Sobchishin LaRhonda , Ruzzini Antonio C. , Simko Elemir 
Published: May 2022

AbstractPaenibacillus larvae, the causative agent of American foulbrood (AFB), produces spores that may be detectable within honey. We analyzed the spore content of pooled, extracted honey from 52 large-scale (L) and 64 small-scale (S) Saskatchewan beekeepers over a two-year period (2019–2020). Our objectives were: (i) establish reliable prognostic reference ranges for spore concentrations in extracted honey to determine future AFB risk at the apiary level; (ii) identify management practices as targets for mitigation of risk. P. larvae spores were detected in 753 of 1476 samples (51%). Beekeepers were stratified into low (< 2 spores/gram), moderate (2- < 100 spores/gram), and high (≥ 100 spores/gram) risk categories. Of forty-nine L beekeepers sampled in 2019, those that reported AFB in 2020 included 0/26 low, 3/18 moderate, and 3/5 high risk. Of twenty-seven L beekeepers sampled in 2020, those that reported AFB in 2021 included 0/11 low, 2/14 moderate, and 1/2 high risk. Predictive modelling included indoor overwintering of hives, purchase of used equipment, movement of honey-producing colonies between apiaries, beekeeper demographic, and antimicrobial use as risk category predictors. Saskatchewan beekeepers with fewer than 2 spores/gram in extracted honey that avoid high risk activities may be considered at low risk of AFB the following year.
Comprehensive Analysis of Prognostic and immune infiltrates for FOXPs Transcription Factors in Human Breast Cancer
Authors: Yi Jianing , Tan Siyi , Zeng Yuanjun , Zou Lianhong , Zeng Jie , Zhang Chaojie , Liu Luyao , Fan Peizhi 
Published: May 2022

AbstractForkhead-box-P family include FOXP1/2/3/4 and its clinical significance still remains unclear in breast cancer (BRCA). We analysed the expressions of FOXPs in BRCA patients to determine diagnostic and prognostic values. Our results indicated that the transcriptional levels of FOXP3/4 were up-regulated in BRCA patients, but FOXP2 were down-regulated. No statistically significant correlation were found between the expression levels of FOXPs in Pathologic stage. FOXP2/3 had a significantly high AUC value in the detection of breast cancer, with 96.8% or 95.7% in accuracy respectively. Our study also suggested that BRCA patients with high transcription levels of FOXP1/2/4 were significantly associated with longer Overall Survival (OS). In contrast, BRCA patients with high transcription level of FOXP3 was not statistically related with OS. Our work revealed that FOXPs were closely related to the alteration of extensive immune checkpoints in breast invasive carcinoma. Additionally, FOXP3 has a significant positive correlation with PDCD1, CD274, CTLA4 and TMB in breast cancer, and FOXP3 expression showed a statistically significant correlation with infiltration of immune cells. Finally, we found that FOXP3 expression predicted the breast cancer cells response to anticancer drugs. Altogether, our work strongly suggested that FOXPs could serve as a biomarker for tumor detection, therapeutic design and prognosis.
Agreements between mean arterial pressure from radial and femoral artery measurements in refractory shock patients
Authors: Wisanusattra Hemmawan , Khwannimit Bodin 
Published: May 2022

AbstractRadial and femoral artery catheterization is the most common procedure for monitoring patients with shock. However, a disagreement in mean arterial pressure (MAP) between the two sites has been reported. Hence, the aim of this study was to compare the MAP from the radial artery (MAPradial) with that of the femoral artery (MAPfemoral) in patients with refractory shock. A prospective study was conducted in the medical intensive care unit. The radial and femoral were simultaneously measured MAP in the patients every hour, for 24 h. In total, 706 paired data points were obtained from 32 patients. MAPradial strongly correlated with MAPfemoral (r = 0.89, p < 0.0001). However, overall MAPradial was significantly lower than MAPfemoral 7.6 mmHg. The bias between MAPradial and MAPfemoral was − 7.6 mmHg (95% limits of agreement (LOA), − 24.1 to 8.9). In the subgroup of patients with MAPradial < 65 mmHg, MAPradial moderately correlated with MAPfemoral (r = 0.63) and the bias was increased to − 13.0 mmHg (95% LOA, − 28.8 to 2.9). There were 414 (58.6%) measurements in which the MAP gradient between the two sites was > 5 mmHg. In conclusion, the radial artery significantly underestimated MAP compared with the femoral artery in patients with refractory shock.
The importance of extrinsic and intrinsic compensatory mechanisms to body posture of competitive athletes a systematic review and meta-analysis
Authors: Zwierzchowska Anna , Gaweł Eliza , Maszczyk Adam , Roczniok Robert 
Published: May 2022

AbstractThe aim of this systematic review (qualitative analysis) was to identify the variables of changes induced by extrinsic (sport specific training) and intrinsic (individual anatomical predispositions) compensatory mechanisms that impact on the physiological magnitude of spinal curvatures in the sagittal plane and their deviations in the frontal plane. Furthermore, the aim of the quantitative analysis was to verify and objectivize the impact of these variables on athlete’s body posture. A search of electronic database (PubMed, EBSCO, MEDLINE) was conducted to identify all studies on sports training and athlete’s spine and body posture from 2011 to 2021. In the sagittal plane, the pooled proportion accounted for 44.97% (95% CI 31.22–58.72%) for thoracic hyperkyphosis (TH), 4.98% (95% CI 1.60–8.36%) for lumbar hyperlordosis (hyperLL), and 12.35% (95% CI 1.60–8.36%) for lumbar hypolordosis (hypoLL). Furthermore, in the sagittal plane, the pooled mean of thoracic kyphosis angle was 37.59° (95% CI 34.45–40.73%), whereas lumbar lordosis angle was 29.79° (95% CI 26.46–33.12%). Professional athletes tend to have postural disturbances and/or spinal curvature disorders in the sagittal and frontal planes. The meta-analysis indicated which intrinsic and extrinsic components might induce spinal abnormalities.
Cancer Awareness Measure (CAM) and Cancer Awareness Measure MYthical Causes Scale (CAM-MY) scores in Pakistani population
Authors: Munir Rimsha , Noureen Naila , Bashir Muniba , Shoaib Naila , Ashraf Arifa , Lisec Jan , Zaidi Nousheen 
Published: May 2022

AbstractLifestyle modifications could prevent almost one-third to one-half of all cancer cases. The awareness of cancer risk factors could motivate people to make such changes in their behaviors and lifestyles. This work aims to investigate the cancer awareness level in the Pakistani population. Telephone interviews of 657 individuals in Pakistan were carried out using the Cancer Awareness Measure (CAM) and Cancer Awareness Measure–MYthical Causes Scale (CAM-MY). We observed that participants scored significantly better on the CAM scale than the CAM-MY scale, and CAM scores were negatively associated with CAM-MY scores. Years of formal education or a biology major at undergraduate or graduate level did not affect our population's cancer awareness levels. Age displayed a weak but statistically significant negative association with CAM scores. Most participants failed to identify modifiable cancer risk factors, e.g., low physical activity. Efforts should be made to improve awareness of modifiable risk factors. We observed that brief training sessions could markedly improve people's understanding of cancer risk factors and myths.
MRA Toolbox v. 1.0: a web-based toolbox for predicting mixture toxicity of chemical substances in chemical products
Authors: Kim Jongwoon , Seo Myungwon , Choi Jiwon , Na Minju 
Published: May 2022

AbstractThe chemical risk assessment paradigm is shifting from “substance-based” to “product/mixture-based” and from “animal testing” to “alternative testing” under chemical regulations. Organisms and the environment may be exposed to mixtures rather than a single substance. Conducting toxicity tests for all possible combinations is impractical due to the enormous combinatorial complexity. This study highlights the development and application case studies of Mixture Risk Assessment Toolbox, a novel web-based platform that supports mixture risk assessment through the use of different prediction models and public databases. This integrated framework provides new functional values for assessors to easily screen and compare the toxicity of mixture products using different computational techniques and find strategic solutions to reduce the mixture toxicity in the product development process. The toolbox (https://www.mratoolbox.org) includes four additive toxicity models: two conventional (Concentration Addition; and Independent Action) and two advanced (Generalized Concentration Addition; and Quantitative Structure–Activity Relationship-based Two-Stage Prediction) models. We demonstrated the multiple functions of the toolbox using three cases: (i) how it can be used to calculate the mixture toxicity, (ii) those for which safety data sheet (SDS) only indicating representative toxicity values (EC50; and LC50), and (iii) those comprising chemicals with low toxic effects.
The optimal duration for the wrinkle test in a clinical setting
Authors: Grinbaum Erez , Shahwan Ahmad , Eliyahu Amir , Shay Ravit , Rozen Nimrod , Rubin Guy 
Published: May 2022

AbstractTo determine the time needed or the development of a positive result on the wrinkle test among patients with complete laceration of a digital nerve in a clinical setting. We prospectively recruited 20 patients who had undergone surgery for digital nerve laceration. The wrinkle test was conducted at a follow-up session up to 2 months after surgery, and the time to a positive wrinkling result was recorded. The wrinkle test was compared between the patient’s injured versus uninjured contralateral finger. The average time required for a positive result on the wrinkle test was 24.5 min (± 11), with 25% patients requiring 40 min to obtain a positive result. When evaluating a patient with digital nerve injury in a "non-laboratory" environment, the wrinkle test may require up to 40 min to obtain a positive result. Our study suggests that if tests are completed following the generally accepted time limit reported in the literature (30 min), up to 25% of tests may produce false negative results.
Quantitative evaluation of emphysema for predicting immunotherapy response in patients with advanced non-small-cell lung cancer
Authors: Noda Yoshimi , Shiroyama Takayuki , Masuhiro Kentaro , Amiya Saori , Enomoto Takatoshi , Adachi Yuichi , Hara Reina , Niitsu Takayuki , Naito Yujiro , Miyake Kotaro , Koyama Shohei , Hirata Haruhiko , Nagatomo Izumi , Takeda Yoshito , Kumanogoh Atsushi 
Published: May 2022

AbstractThe efficacy of immune checkpoint inhibitors (ICIs) in patients with advanced non-small-cell lung cancer (NSCLC) might depend on the presence of emphysema, but this association is not established. We aimed to investigate if quantitively and automatically measuring emphysema can predict the effect of ICIs. We retrospectively analyzed 56 patients with NSCLC who underwent immunotherapy at our hospital. We used the Goddard scoring system (GS) to evaluate the severity of emphysema on baseline CT scans using three-dimensional image analysis software. The emphysema group (GS ≥ 1) showed better progression-free survival (PFS) than the non-emphysema group (GS = 0) (6.5 vs. 2.3 months, respectively, p < 0.01). Multivariate analyses revealed that good performance status, GS of ≥ 1, and high expression of PD-L1 were independently associated with better PFS, while smoking status was not. In conclusion, quantitative evaluation of emphysema can be an objective parameter for predicting the therapeutic effects of ICIs in patients with NSCLC. Our findings can be used to generate hypotheses for future studies.
Nsun4 and Mettl3 mediated translational reprogramming of Sox9 promotes BMSC chondrogenic differentiation
Authors: Yang Lin , Ren Zhenxing , Yan Shenyu , Zhao Ling , Liu Jie , Zhao Lijun , Li Zhen , Ye Shanyu , Liu Aijun , Li Xichan , Guo Jiasong , Zhao Wei , Kuang Weihong , Liu Helu , Chen Dongfeng 
Published: May 2022

AbstractThe chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) has been used in the treatment and repair of cartilage defects; however, the in-depth regulatory mechanisms by which RNA modifications are involved in this process are still poorly understood. Here, we found that Sox9, a critical transcription factor that mediates chondrogenic differentiation, exhibited enhanced translation by ribosome sequencing in chondrogenic pellets, which was accompanied by increased 5-methylcytosine (m5C) and N6-methyladenosine (m6A) levels. Nsun4-mediated m5C and Mettl3-mediated m6A modifications were required for Sox9-regulated chondrogenic differentiation. Interestingly, we showed that in the 3’UTR of Sox9 mRNA, Nsun4 catalyzed the m5C modification and Mettl3 catalyzed the m6A modification. Furthermore, we found that Nsun4 and Mettl3 co-regulated the translational reprogramming of Sox9 via the formation of a complex. Surface plasmon resonance (SPR) assays showed that this complex was assembled along with the recruitment of Ythdf2 and eEF1α-1. Moreover, BMSCs overexpressing Mettl3 and Nsun4 can promote the repair of cartilage defects in vivo. Taken together, our study demonstrates that m5C and m6A co-regulate the translation of Sox9 during the chondrogenic differentiation of BMSCs, which provides a therapeutic target for clinical implications.
High efficiency laser-driven proton sources using 3D-printed micro-structure
Authors: Qin Chengyu , Zhang Hui , Li Shun , Wang Nengwen , Li Angxiao , Fan Lulin , Lu Xiaoming , Li Jinfeng , Xu Rongjie , Wang Cheng , Liang Xiaoyan , Leng Yuxin , Shen Baifei , Ji Liangliang , Li Ruxin 
Published: May 2022

AbstractFine structured targets are promising in enhancing laser-driven proton acceleration for various applications. Here, we apply 3D-printed microwire-array (MWA) structure to boost the energy conversion efficiency from laser to proton beam. Under irradiation of high contrast femtosecond laser pulse, the MWA target generates over 1.2 × 1012 protons (>1 MeV) with cut-off energies extending to 25 MeV, corresponding to top-end of 8.7% energy conversion efficiency. When comparing to flat foils the efficiency is enhanced by three times, while the cut-off energy is increased by 32%. We find the dependence of proton energy/conversion-efficiency on the spacing of the MWA. The experimental trend is well reproduced by hydrodynamic and Particle-In-Cell simulations, which reveal the modulation of pre-plasma profile induced by laser diffraction within the fine structures. Our work validates the use of 3D-printed micro-structures to produce high efficiency laser-driven particle sources and pointed out the effect in optimizing the experimental conditions.
Research on integrated safety assessment model of electric vehicle charging process
Authors: Sun Lei , Ma Yong , Li Xiaohan , Guo Dongliang , Liu Jianjun , Wu Peng , Xiao Peng 
Published: May 2022

AbstractWith the rapid development of electric vehicles (EV), the problem of charging safety is a multifaceted and complex issue. Taking the integration of electric vehicle charging as the research object, including power batteries, charging piles, and power distribution grids, charging data is collected based on data mining technology. At the same time, the factors affecting charging safety are analysed, and an integrated index set is established and optimised. The comprehensive fuzzy evaluation method is used to comprehensively analyse the monitoring data of the electric vehicle charging process, the weight is determined based on the grey correlation method and the expert scoring mechanism, and an integrated evaluation model of the electric vehicle charging process is established. Analyse five sets of charging data in Nanjing through calculation examples, and output the integrated health degree of the electric vehicle charging process so that the equipment can be maintained in a targeted manner, which effectively proves the practicability and reliability of the assessment model.
Piezo-optomechanical cantilever modulators for VLSI visible photonics
Authors: Dong Mark , Heim David , Witte Alex , Clark Genevieve , Leenheer Andrew J. , Dominguez Daniel , Zimmermann Matthew , Wen Y. Henry , Gilbert Gerald , Englund Dirk , Eichenfield Matt 
Published: May 2022

Visible-wavelength very large-scale integration photonic circuits have a potential to play important roles in quantum information and sensing technologies. The realization of scalable, high-speed, and low-loss photonic mesh circuits depends on reliable and well-engineered visible photonic components. Here, we report a low-voltage optical phase shifter based on piezo-actuated mechanical cantilevers, fabricated on a CMOS compatible, 200 mm wafer-based visible photonics platform. We show linear phase and amplitude modulation with 6 Vπ cm in differential operation, −1.5 to −2 dB insertion loss, and up to 40 dB contrast in the 700–780 nm range. By adjusting selected cantilever parameters, we demonstrate a low-displacement and a high-displacement device, both exhibiting a nearly flat frequency response from DC to a peak mechanical resonance at 23 and 6.8 MHz respectively, which, through resonant enhancement of Q ∼ 40, further decreases the operating voltage down to 0.15 Vπ cm.