bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extremely wet summer events enhance permafrost thaw for multiple years in Siberian tundra
Authors: Magnússon Rúna Í. , Hamm Alexandra , Karsanaev Sergey V. , Limpens Juul , Kleijn David , Frampton Andrew , Maximov Trofim C. , Heijmans Monique M. P. D. 
Published: March 2022

AbstractPermafrost thaw can accelerate climate warming by releasing carbon from previously frozen soil in the form of greenhouse gases. Rainfall extremes have been proposed to increase permafrost thaw, but the magnitude and duration of this effect are poorly understood. Here we present empirical evidence showing that one extremely wet summer (+100 mm; 120% increase relative to average June–August rainfall) enhanced thaw depth by up to 35% in a controlled irrigation experiment in an ice-rich Siberian tundra site. The effect persisted over two subsequent summers, demonstrating a carry-over effect of extremely wet summers. Using soil thermal hydrological modelling, we show that rainfall extremes delayed autumn freeze-up and rainfall-induced increases in thaw were most pronounced for warm summers with mid-summer precipitation rainfall extremes. Our results suggest that, with rainfall and temperature both increasing in the Arctic, permafrost will likely degrade and disappear faster than is currently anticipated based on rising air temperatures alone.
Perovskite metasurfaces with large superstructural chirality
Authors: Long Guankui , Adamo Giorgio , Tian Jingyi , Klein Maciej , Krishnamoorthy Harish N. S. , Feltri Elena , Wang Hebin , Soci Cesare 
Published: March 2022

AbstractRecent attempts to synthesize hybrid perovskites with large chirality have been hampered by large size mismatch and weak interaction between their structure and the wavelength of light. Here we adopt a planar nanostructure design to overcome these limitations and realize all-dielectric perovskite metasurfaces with giant superstructural chirality. We identify a direct spectral correspondence between the near- and the far- field chirality, and tune the electric and magnetic multipole moments of the resonant chiral metamolecules to obtain large anisotropy factor of 0.49 and circular dichroism of 6350 mdeg. Simulations show that larger area metasurfaces could yield even higher optical activity, approaching the theoretical limits. Our results clearly demonstrate the advantages of nanostructrure engineering for the implementation of perovskite chiral photonic, optoelectronic, and spintronic devices.
Rotating neurons for all-analog implementation of cyclic reservoir computing
Authors: Liang Xiangpeng , Zhong Yanan , Tang Jianshi , Liu Zhengwu , Yao Peng , Sun Keyang , Zhang Qingtian , Gao Bin , Heidari Hadi , Qian He , Wu Huaqiang 
Published: March 2022

AbstractHardware implementation in resource-efficient reservoir computing is of great interest for neuromorphic engineering. Recently, various devices have been explored to implement hardware-based reservoirs. However, most studies were mainly focused on the reservoir layer, whereas an end-to-end reservoir architecture has yet to be developed. Here, we propose a versatile method for implementing cyclic reservoirs using rotating elements integrated with signal-driven dynamic neurons, whose equivalence to standard cyclic reservoir algorithm is mathematically proven. Simulations show that the rotating neuron reservoir achieves record-low errors in a nonlinear system approximation benchmark. Furthermore, a hardware prototype was developed for near-sensor computing, chaotic time-series prediction and handwriting classification. By integrating a memristor array as a fully-connected output layer, the all-analog reservoir computing system achieves 94.0% accuracy, while simulation shows >1000× lower system-level power than prior works. Therefore, our work demonstrates an elegant rotation-based architecture that explores hardware physics as computational resources for high-performance reservoir computing.
Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin
Authors: Rivera Carlos , Lee Hun-Goo , Lappala Anna , Wang Danni , Noches Verónica , Olivares-Costa Montserrat , Sjöberg-Herrera Marcela , Lee Jeannie T. , Andrés María Estela 
Published: March 2022

AbstractRCOR1 is a known transcription repressor that recruits and positions LSD1 and HDAC1/2 on chromatin to erase histone methylation and acetylation. However, there is currently an incomplete understanding of RCOR1’s range of localization and function. Here, we probe RCOR1’s distribution on a genome-wide scale and unexpectedly find that RCOR1 is predominantly associated with transcriptionally active genes. Biochemical analysis reveals that RCOR1 associates with RNA Polymerase II (POL-II) during transcription and deacetylates its carboxy-terminal domain (CTD) at lysine 7. We provide evidence that this non-canonical RCOR1 activity is linked to dampening of POL-II productive elongation at actively transcribing genes. Thus, RCOR1 represses transcription in two ways—first, via a canonical mechanism by erasing transcriptionally permissive histone modifications through associating with HDACs and, second, via a non-canonical mechanism that deacetylates RNA POL-II’s CTD to inhibit productive elongation. We conclude that RCOR1 is a transcription rheostat.
Boron clusters as broadband membrane carriers
Authors: Barba-Bon Andrea , Salluce Giulia , Lostalé-Seijo Irene , Assaf Khaleel. I. , Hennig Andreas , Montenegro Javier , Nau Werner M. 
Published: March 2022

AbstractThe membrane translocation of hydrophilic substances constitutes a challenge for their application as therapeutic compounds and labelling probes1–4. To remedy this, charged amphiphilic molecules have been classically used as carriers3,5. However, such amphiphilic carriers may cause aggregation and non-specific membrane lysis6,7. Here we show that globular dodecaborate clusters, and prominently B12Br122−, can function as anionic inorganic membrane carriers for a broad range of hydrophilic cargo molecules (with molecular mass of 146–4,500 Da). We show that cationic and neutral peptides, amino acids, neurotransmitters, vitamins, antibiotics and drugs can be carried across liposomal membranes. Mechanistic transport studies reveal that the carrier activity is related to the superchaotropic nature of these cluster anions8–12. We demonstrate that B12Br122− affects cytosolic uptake of different small bioactive molecules, including the antineoplastic monomethyl auristatin F, the proteolysis targeting chimera dBET1 and the phalloidin toxin, which has been successfully delivered in living cells for cytoskeleton labelling. We anticipate the broad and distinct delivery spectrum of our superchaotropic carriers to be the starting point of conceptually distinct cell-biological, neurobiological, physiological and pharmaceutical studies.
The development and evolution of inhibitory neurons in primate cerebrum
Authors: Schmitz Matthew T. , Sandoval Kadellyn , Chen Christopher P. , Mostajo-Radji Mohammed A. , Seeley William W. , Nowakowski Tomasz J. , Ye Chun Jimmie , Paredes Mercedes F. , Pollen Alex A. 
Published: March 2022

AbstractNeuroanatomists have long speculated that expanded primate brains contain an increased morphological diversity of inhibitory neurons (INs)1, and recent studies have identified primate-specific neuronal populations at the molecular level2. However, we know little about the developmental mechanisms that specify evolutionarily novel cell types in the brain. Here, we reconstruct gene expression trajectories specifying INs generated throughout the neurogenic period in macaques and mice by analysing the transcriptomes of 250,181 cells. We find that the initial classes of INs generated prenatally are largely conserved among mammals. Nonetheless, we identify two contrasting developmental mechanisms for specifying evolutionarily novel cell types during prenatal development. First, we show that recently identified primate-specificTAC3striatal INs are specified by a unique transcriptional programme in progenitors followed by induction of a distinct suite of neuropeptides and neurotransmitter receptors in new-born neurons. Second, we find that multiple classes of transcriptionally conserved olfactory bulb (OB)-bound precursors are redirected to expanded primate white matter and striatum. These classes include a novel peristriatal class of striatum laureatum neurons that resemble dopaminergic periglomerular cells of the OB. We propose an evolutionary model in which conserved initial classes of neurons supplying the smaller primate OB are reused in the enlarged striatum and cortex. Together, our results provide a unified developmental taxonomy of initial classes of mammalian INs and reveal multiple developmental mechanisms for neural cell type evolution.
Parental praise and children’s exploration: a virtual reality experiment
Authors: Brummelman Eddie , Grapsas Stathis , van der Kooij Katinka 
Published: March 2022

AbstractWhen children practice a new skill and fail, it is critical for them to explore new strategies to succeed. How can parents encourage children’s exploration? Bridging insights from developmental psychology and the neuroscience of motor control, we examined the effects of parental praise on children’s motor exploration. We theorize that modest praise can spark exploration. Unlike inflated praise, modest praise acknowledges children’s performance, without setting a high standard for future performance. This may be reassuring to children with lower levels of self-esteem, who often doubt their ability. We conducted a novel virtual-reality experiment. Children (N = 202, ages 8–12) reported self-esteem and performed a virtual-reality 3D trajectory-matching task, with success/failure feedback after each trial. Children received modest praise (“You did well!”), inflated praise (“You did incredibly well!”), or no praise from their parent. We measured motor exploration as children’s tendency to vary their movements following failure. Relative to no praise, modest praise—unlike inflated praise—encouraged exploration in children with lower levels of self-esteem. By contrast, modest praise discouraged exploration in children with higher levels of self-esteem. Effects were small yet robust. This experiment demonstrates that modest praise can spark exploration in children with lower levels of self-esteem.
Long-distance, synchronized and directional fall movements suggest migration in Arctic hares on Ellesmere Island (Canada)
Authors: Caron-Carrier Jacob , Lai Sandra , Vézina François , Tam Andrew , Berteaux Dominique 
Published: March 2022

AbstractAnimal migration contributes largely to the seasonal dynamics of High Arctic ecosystems, linking distant habitats and impacting ecosystem structure and function. In polar deserts, Arctic hares are abundant herbivores and important components of food webs. Their annual migrations have long been suspected, but never confirmed. We tracked 25 individuals with Argos satellite telemetry to investigate the existence of migration in a population living at Alert (Ellesmere Island, Nunavut, Canada). During fall, 21 hares undertook directional, long-distance movements in a southwestern direction towards Lake Hazen. Daily movement rates averaged 1.3 ± 0.5 km, 4.3 ± 1.6 km, and 1.7 ± 0.9 km before, during, and after relocation, respectively. Straight-line and minimum cumulative distances traveled averaged 98 ± 18 km (range: 72–148 km) and 198 ± 62 km (range: 113–388 km), respectively. This is the first report of large-scale seasonal movements in Arctic hares and, surprisingly, in any lagomorph species. These movements may be part of an annual migratory pattern. Our results redefine our understanding of the spatial ecology of Arctic hares, demonstrate unsuspected mobility capacities in lagomorphs, and open new perspectives regarding the ecological dynamics of the northern polar deserts.
Changes in adverse pregnancy outcomes in women with advanced maternal age (AMA) after the enactment of China’s universal two-child policy
Authors: Li Hui , Nawsherwan  , Fan Cuifang , Yin Shanshan , Haq Ijaz ul , Mubarik Sumaira , Nabi Ghulam , Khan Suliman , Hua Linlin 
Published: March 2022

AbstractThe universal two-child policy (TCP; 2016) in China has affected many aspects of maternal-neonatal health. A tertiary hospital-based retrospective study (2011–2019) was used to find the association of these policy changes with maternal age and pregnancy outcomes in women with AMA (≥ 35 years) in the Hubei Province, China. The proportion of neonatal births to women with AMA increased by 68.8% from 12.5% in the one-child policy (OCP) period to 21.1% in the universal TCP period [aOR 1.76 (95% CI: 1.60, 1.93)]. In the univariate analysis, the proportion of preterm births (29.4% to 24.1%), low birth weight (LBW) (20.9% to 15.9%), and hypertensive disorders of pregnancy (HDP) (11.5% to 9.2%) significantly (p < 0.05) decreased in women with AMA from the OCP period to universal TCP period. However, the proportion of intrauterine growth restriction (IUGR) (0.2% to 0.7%) and gestational diabetes mellitus (GDM) (1.7% to 15.6%) was significantly (p < 0.05) increased over the policy changes. After adjusting for confounding factors, only the risk of GDM increased [aOR 10.91 (95% CI: 6.05, 19.67)] in women with AMA from the OCP period to the universal TCP period. In conclusion, the risk of GDM increased in women with AMA from the OCP period to the universal TCP period.
Metabolomics-based profiling of 4 avocado varieties using HPLC–MS/MS and GC/MS and evaluation of their antidiabetic activity
Authors: Younis Inas Y. , Khattab Amira R. , Selim Nabil M. , Sobeh Mansour , Elhawary Seham S. , Bishbishy Mahitab H. El 
Published: March 2022

AbstractSeven avocado “Persea americana” seeds belonging to 4 varieties, collected from different localities across the world, were profiled using HPLC–MS/MS and GC/MS to explore the metabolic makeup variabilities and antidiabetic potential. For the first time, 51 metabolites were tentatively-identified via HPLC–MS/MS, belonging to different classes including flavonoids, biflavonoids, naphthodianthrones, dihydrochalcones, phloroglucinols and phenolic acids while 68 un-saponified and 26 saponified compounds were identified by GC/MS analysis. The primary metabolic variabilities existing among the different varieties were revealed via GC/MS-based metabolomics assisted by unsupervised pattern recognition methods. Fatty acid accumulations were proved as competent, and varietal-discriminatory metabolites. The antidiabetic potential of the different samples was explored using in-vitro amylase and glucosidase inhibition assays, which pointed out to Gwen (KG) as the most potent antidiabetic sample. This could be attributed to its enriched content of poly-unsaturated fatty acids and polyphenolics. Molecular docking was then performed to predict the most promising phytoligands in KG variety to be posed as antidiabetic drug leads. The highest in-silico α-amylase inhibition was observed with chrysoeriol-4′-O-pentoside-7-O-rutinoside, apigenin-7-glucuronide and neoeriocitrin which might serve as potential drug leads for the discovery of new antidiabetic remedies.
An efficient density peak cluster algorithm for improving policy evaluation performance
Authors: Yu Zhenhua , Yan Yanghao , Deng Fan , Zhang Fei , Li Zhiwu 
Published: March 2022

AbstractIn recent years, the XACML (eXtensible Access Control Markup Language) is widely used in a variety of research fields, especially in access control. However, when policy sets defined by the XACML become large and complex, the policy evaluation time increases significantly. In order to improve policy evaluation performance, we propose an optimization algorithm based on the DPCA (Density Peak Cluster Algorithm) to improve the clustering effect on large-scale complex policy sets. Combined with this algorithm, an efficient policy evaluation engine, named DPEngine, is proposed to speed up policy matching and reduce the policy evaluation time. We compare the policy evaluation time of DPEngine with the Sun PDP, HPEngine, XEngine and SBA-XACML. The experiment results show that (1) when the number of requests reaches 10,000, the DPEngine evaluation time on a large-scale policy set with 100,000 rules is approximately 2.23%, 3.47%, 3.67% and 4.06% of that of the Sun PDP, HPEngine, XEngine and SBA-XACML, respectively and (2) as the number of requests increases, the DPEngine evaluation time grows linearly. Compared with other policy evaluation engines, the DPEngine has the advantages of efficiency and stability.
Mandible shape variation and feeding biomechanics in minks
Authors: Gálvez-López Eloy , Cox Philip G. 
Published: March 2022

AbstractEuropean and American minks are very similar in ecology, behavior and morphology. Both species hunt terrestrial vertebrates and aquatic prey, but the American mink is a more generalist predator which, among other factors, allows it to outcompete the European mink in areas where it has been introduced. We used 3D geometric morphometrics and estimates of muscle mechanical advantage to assess the degree of variation in mandibular morphology, and to determine whether such variation reflects dietary differences between the two species. The three main axes of variation represented interspecific differences, a common allometric trajectory between species and sexes, and the interspecific effect of sexual size dimorphism, with males having overall stronger bites than females. Differences in mandible shape and biomechanical parameters suggest that American minks are better equipped for preying on terrestrial vertebrates, while the features seen in European mink could be related to tougher prey, fish capture, or both. Additionally, within each species, the larger specimens of each sex present indicators of a higher percentage of terrestrial prey in their diet. These results indicate a low potential dietary overlap between both species, suggesting that factors other than prey competition may have a role in the decline of the European mink.
Diversity of the lysozyme fold: structure of the catalytic domain from an unusual endolysin encoded by phage Enc34
Authors: Cernooka Elina , Rumnieks Janis , Zrelovs Nikita , Tars Kaspars , Kazaks Andris 
Published: March 2022

AbstractEndolysins are bacteriophage-encoded peptidoglycan-degrading enzymes with potential applications for treatment of multidrug-resistant bacterial infections. Hafnia phage Enc34 encodes an unusual endolysin with an N-terminal enzymatically active domain and a C-terminal transmembrane domain. The catalytic domain of the endolysin belongs to the conserved protein family PHA02564 which has no recognizable sequence similarity to other known endolysin types. Turbidity reduction assays indicate that the Enc34 enzyme is active against peptidoglycan from a variety of Gram-negative bacteria including the opportunistic pathogen Pseudomonas aeruginosa PAO1. The crystal structure of the catalytic domain of the Enc34 endolysin shows a distinctive all-helical architecture that distantly resembles the α-lobe of the lysozyme fold. Conserved catalytically important residues suggest a shared evolutionary history between the Enc34 endolysin and GH73 and GH23 family glycoside hydrolases and propose a molecular signature for substrate cleavage for a large group of peptidoglycan-degrading enzymes.
Territorial bias in university rankings: a complex network approach
Authors: Bellantuono Loredana , Monaco Alfonso , Amoroso Nicola , Aquaro Vincenzo , Bardoscia Marco , Loiotile Annamaria Demarinis , Lombardi Angela , Tangaro Sabina , Bellotti Roberto 
Published: March 2022

AbstractUniversity rankings are increasingly adopted for academic comparison and success quantification, even to establish performance-based criteria for funding assignment. However, rankings are not neutral tools, and their use frequently overlooks disparities in the starting conditions of institutions. In this research, we detect and measure structural biases that affect in inhomogeneous ways the ranking outcomes of universities from diversified territorial and educational contexts. Moreover, we develop a fairer rating system based on a fully data-driven debiasing strategy that returns an equity-oriented redefinition of the achieved scores. The key idea consists in partitioning universities in similarity groups, determined from multifaceted data using complex network analysis, and referring the performance of each institution to an expectation based on its peers. Significant evidence of territorial biases emerges for official rankings concerning both the OECD and Italian university systems, hence debiasing provides relevant insights suggesting the design of fairer strategies for performance-based funding allocations.
CRISPR-mediated knockout of cardinal and cinnabar eye pigmentation genes in the western tarnished plant bug
Authors: Heu Chan C. , Gross Roni J. , Le Kevin P. , LeRoy Dannialle M. , Fan Baochan , Hull J. Joe , Brent Colin S. , Fabrick Jeffrey A. 
Published: March 2022

AbstractThe western tarnished plant bug, Lygus hesperus, is a key hemipteran pest of numerous agricultural, horticultural, and industrial crops in the western United States and Mexico. A lack of genetic tools in L. hesperus hinders progress in functional genomics and in developing innovative pest control methods such as gene drive. Here, using RNA interference (RNAi) against cardinal (LhCd), cinnabar (LhCn), and white (LhW), we showed that knockdown of LhW was lethal to developing embryos, while knockdown of LhCd or LhCn produced bright red eye phenotypes, in contrast to wild-type brown eyes. We further used CRISPR/Cas9 (clustered regularly interspaced palindromic repeats/CRISPR-associated) genome editing to generate germline knockouts of both LhCd (Card) and LhCn (Cinn), producing separate strains of L. hesperus characterized by mutant eye phenotypes. Although the cardinal knockout strain Card exhibited a gradual darkening of the eyes to brown typical of the wild-type line later in nymphal development, we observed bright red eyes throughout all life stages in the cinnabar knockout strain Cinn, making it a viable marker for tracking gene editing in L. hesperus. These results provide evidence that CRISPR/Cas9 gene editing functions in L. hesperus and that eye pigmentation genes are useful for tracking the successful genetic manipulation of this insect.
The monoacylglycerol acyltransferase pathway contributes to triacylglycerol synthesis in HepG2 cells
Authors: McFie Pamela J. , Patel Apurv , Stone Scot J. 
Published: March 2022

AbstractThe monoacylglycerol acyltransferase (MGAT) pathway has a well-established role in the small intestine where it facilitates the absorption of dietary fat. In enterocytes, MGAT participates in the resynthesis of triacylglycerol using substrates (monoacylglycerol and fatty acids) generated in the gut lumen from the breakdown of triacylglycerol consumed in the diet. MGAT activity is also present in the liver, but its role in triacylglycerol metabolism in this tissue remains unclear. The predominant MGAT isoforms present in human liver appear to be MGAT2 and MGAT3. The objective of this study was to use selective small molecule inhibitors of MGAT2 and MGAT3 to determine the contributions of these enzymes to triacylglycerol production in liver cells. We found that pharmacological inhibition of either enzyme had no effect on TG mass in HepG2 cells but did alter lipid droplet size and number. Inhibition of MGAT2 did result in decreased DG and TG synthesis and TG secretion. Interestingly, MGAT2 preferentially utilized 2-monoacylglycerol derived from free glycerol and not from exogenously added 2-monoacylglycerol. In contrast, inhibition of MGAT3 had very little effect on TG metabolism in HepG2 cells. Additionally, we demonstrated that the MGAT activity of DGAT1 only makes a minor contribution to TG synthesis in intact HepG2 cells. Our data demonstrated that the MGAT pathway has a role in hepatic lipid metabolism with MGAT2, more so than MGAT3, contributing to TG synthesis and secretion.