bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensitivity of the Cervical Disc Loads, Translations, Intradiscal Pressure, and Muscle Activity Due to Segmental Mass, Disc Stiffness, and Muscle Strength in an Upright Neutral Posture
Authors: Arshad Rizwan , Schmidt Hendrik , El-Rich Marwan , Moglo Kodjo 
Published: April 2022

Musculoskeletal disorders of the cervical spine have increased considerably in recent times. To understand the effects of various biomechanical factors, quantifying the differences in disc loads, motion, and muscle force/activity is necessary. The kinematic, kinetic, or muscle response may vary in a neutral posture due to interindividual differences in segmental mass, cervical disc stiffness, and muscle strength. Therefore, our study aimed to develop an inverse dynamic model of the cervical spine, estimate the differences in disc loads, translations, intradiscal pressure, and muscle force/activity in a neutral posture and compare these results with data available in the literature. A head–neck complex with nine segments (head, C1–T1) was developed with joints having three rotational and three translational degrees of freedom, 517 nonlinear ligament fibers, and 258 muscle fascicles. A sensitivity analysis was performed to calculate the effect of segmental mass (5th to 95th percentile), translational disc stiffness (0.5–1.5), and muscle strength (0.5–1.5) on the cervical disc loads (C2–C3 to C7–T1), disc translations, intradiscal pressure, and muscle force/activity in a neutral posture. In addition, two axial external load conditions (0 and 40 N) were also considered on the head. The estimated intradiscal pressures (0.2–0.56 MPa) at 0 N axial load were comparable to in vivo measurements found in the literature, whereas at 40 N, the values were 0.39–0.93 MPa. With increased segmental mass (5th to 95th), the disc loads, translations, and muscle forces/activities increased to 69% at 0 N and 34% at 40 N axial load. With increased disc stiffness (0.5–1.5), the maximum differences in axial (&lt;1%) and shear loads (4%) were trivial; however, the translations were reduced by 67%, whereas the differences in individual muscle group forces/activities varied largely. With increased muscle strength (0.5–1.5), the muscle activity decreased by 200%. For 40 vs. 0 N, the differences in disc loads, translations, and muscle forces/activities were in the range of 52–129%. Significant differences were estimated in disc loads, translations, and muscle force/activity in the normal population, which could help distinguish between normal and pathological cervical spine conditions.
Interfacial Assemble of Prussian Blue Analog to Access Hierarchical FeNi (oxy)-Hydroxide Nanosheets for Electrocatalytic Water Splitting
Authors: Hong Jinquan , Lv Jiangquan , Chen Jialing , Cai Lanxin , Wei Mengna , Cai Guoseng , Huang Xin , Li Xiaoyan , Du Shaowu 
Published: April 2022

Developing facile methods for the synthesis of active and stable electrocatalysts is vitally important to realize overall water splitting. Here, we demonstrate a practical method to obtain FeNiOOH nanosheets on nickel foam (NF) as bifunctional electrocatalyst by growing a FeCo Prussian blue analog with further in situ oxidation under ambient conditions. The binder-free, self-standing FeNiOOH/NF electrode with hierarchical nanostructures requires low overpotentials of 260 mV and 240 mV at a current density of 50 mA cm−2 for oxygen evolution reaction and hydrogen evolution reaction, respectively, in 1.0 M KOH solution. Therefore, an alkaline water electrolyzer constructed by bifunctional FeNiOOH/NF electrode as both anode and cathode delivers 50 mA cm−2 under a cell voltage of 1.74 V with remarkable stability, which outperforms the IrO2-Pt/C-based electrolyzer. The excellent performance could be ascribed to the superior FeNiOOH intrinsic activity and the hierarchical structure. This work provides a cost-efficient surface engineering method to obtain binder-free, self-standing bifunctional electrocatalyst on commercial NF, which could be further extended to other energy and environment applications.
Development and Validation of a Risk Score for Predicting Invasive Candidiasis in Intensive Care Unit Patients by Incorporating Clinical Risk Factors and Lymphocyte Subtyping
Authors: Zhang Jiahui , Cheng Wei , Li Dongkai , Chen Jianwei , Zhao Guoyu , Wang Hao , Cui Na 
Published: April 2022

ObjectiveTo develop and validate a rapid invasive candidiasis (IC)-predictive risk score in intensive care unit (ICU) patients by incorporating clinical risk factors and parameters of lymphocyte subtyping.MethodsA prospective cohort study of 1054 consecutive patients admitted to ICU was performed. We assessed the clinical characteristics and parameters of lymphocyte subtyping at the onset of clinical signs of infection and their potential influence on IC diagnosis. A risk score for early diagnosis of IC was developed and validated based on a logistic regression model.ResultsSixty-nine patients (6.5%) had IC. Patients in the cohort (N=1054) were randomly divided into a development (n=703) or validation (n=351) cohorts. Multivariate logistic regression identified that CD8+ T-cell count ≤143 cells/mm3, receipt of high-dose corticosteroids (dose ≥50 mg prednisolone equivalent), receipt of carbapenem/tigecycline, APACHE II score≥15, (1,3)-β-D-glucan (BDG) positivity and emergency gastrointestinal/hepatobiliary (GIT/HPB) surgery were significantly related with IC. IC risk score was calculated using the following formula: CD8+ T-cell count ≤143 cells/mm3 + receipt of high-dose corticosteroids + receipt of carbapenem/tigecycline + APACHE II score ≥15 + BDG positivity + emergency GIT/HPB surgery ×2. The risk scoring system had good discrimination and calibration with area under the receiver operating characteristic (AUROC) curve of 0.820 and 0.807, and a non-significant Hosmer-Lemeshow test P=0.356 and P=0.531 in the development and validation cohorts, respectively. We categorized patients into three groups according to risk score: low risk (0-2 points), moderate risk (3-4 points) and high risk (5-7 points). IC risk was highly and positively associated with risk score (Pearson contingency coefficient=0.852, P for trend=0.007). Candida score had a moderate predicting efficacy for early IC diagnosis. The AUROC of the risk score was significantly larger than that of Candida score (0.820 versus 0.711, Z=2.013, P=0.044).ConclusionsThe predictive scoring system, which used both clinical factors and CD8+ T cell count, served as a clinically useful predictive model for rapid IC diagnosis in this cohort of ICU patients.Clinical Trial Registrationchictr.org.cn, identifier ChiCTR-ROC-17010750.
Epidemiology of Congenital Heart Disease in Jinan, China From 2005 to 2020: A Time Trend Analysis
Authors: Zhang Lihua , Liu Bei , Li Huimin , Wang Chengxiang , Yang Shimin , Li Zhongliang 
Published: April 2022

BackgroundAlthough congenital heart defect (CHD) was the dominating birth defect, the time trend analysis of CHD was largely unknown. In our study, the time trend analysis of CHD from 2005 to 2020 in Jinan was conducted, aimed to reveal the epidemiological characteristics in a city and provided the data basis for the government to make a policy intervention.MethodsA multi-institutional and retrospective review of CHD for all births from January 1, 2005 to December 31, 2020 was performed. Proportioner prevalence was used to describe the distribution of CHD. Comparisons of CHD characteristics among different groups were assessed with Chi-squares tests. Cochran-Armitage tests (CAT) were used to track changes in CHD prevalence.ResultsAbout 322,374 births and 5,180 CHD in Jinan were included from 2005 to 2020, and the total CHD prevalence was 3.92 per 1,000 births. The CHD prevalence showed an upward trend, with a total increase of 227.66% from 2005 to 2020. The CHD prevalence in urban areas was 34.17% higher than that in rural areas, but the gap was narrowing. Atrial septal defect (3.07 per 1,000 births), patent ductus arteriosus (1.62 per 1,000 births), ventricular septal defect (1.18 per 1,000 births), tetralogy of Fallot (0.62 per 1,000 births), and atrioventricular septal defect (0.47 per 1,000 births) were the 5 most common subtypes.ConclusionThe prevalence of CHD in Jinan was gradually on the rise, which needs to be highly focused on by the health management department. Older pregnant women and women in rural areas should be concerned, and targeted measures need to be introduced.
Left Heart Chamber Volumetric Assessment by Automated Three-Dimensional Echocardiography in Heart Transplant Recipients
Authors: Zhang Yiwei , Wu Chun , Sun Wei , Zhu Shuangshuang , Zhang Yanting , Xie Yuji , Zhu Ye , Zhang Zisang , Zhao Yang , Li Yuman , Xie Mingxing , Zhang Li 
Published: April 2022

BackgroundRecently, a new automated software (Heart Model) was developed to obtain three-dimensional (3D) left heart chamber volumes. The aim of this study was to verify the feasibility and accuracy of the automated 3D echocardiographic algorithm in heart transplant (HTx) patients. Conventional manual 3D transthoracic echocardiographic (TTE) tracings and cardiac magnetic resonance (CMR) images were used as a reference for comparison.MethodsThis study enrolled 103 healthy HTx patients prospectively. In protocol 1, left ventricular end-diastolic volume (LVEDV), LV end-systolic volume (LVESV), left atrial max volume (LAVmax), LA minimum volume (LAVmin) and LV ejection fraction (LVEF) were obtained using the automated 3D echocardiography (3DE) and compared with corresponding values obtained through the manual 3DE. In protocol 2, 28 patients’ automated 3DE measurements were compared with CMR reference values. The impacts of contour edit and surgical technique were also tested.ResultsHeart Model was feasible in 97.1% of the data sets. In protocol 1, there was strong correlation between 3DE and manual 3DE for all the parameters (r = 0.77 to 0.96, p&lt;0.01). Compared to values obtained through manual measurements, LV volumes and LVEF were overestimated by the automated algorithm and LA volumes were underestimated. All the biases were small except for that of LAVmin. After contour adjustment, the biases reduced and all the limits of agreement were clinically acceptable. In protocol 2, the correlations for LV and LA volumes were strong between automated 3DE with contour edit and CMR (r = 0.74 to 0.93, p&lt;0.01) but correlation for LVEF remained moderate (r = 0.65, p &lt; 0.01). Automated 3DE overestimated LV volumes but underestimated LVEF and LA volumes compared with CMR. The limits of agreement were clinically acceptable only for LVEDV and LAVmax.ConclusionSimultaneous quantification of left heart volumes and LVEF with the automated Heart Model program is rapid, feasible and to a great degree it is accurate in HTx recipients. Nevertheless, only LVEDV and LAVmax measured by automated 3DE with contour edit seem applicable for clinical practice when compared with CMR. Automated 3DE for HTx recipients is a worthy attempt, though further verification and optimization are needed.
Establishment and Validation of a Multivariate Predictive Scoring Model for Intravenous Immunoglobulin-Resistant Kawasaki Disease: A Study of Children From Two Centers in China
Authors: Li Changjian , Wu Shu , Shi Yuanyuan , Liao Ying , Sun Yan , Yan Hui , Zhang Qingyou , Fu Jia , Zhou Dan , Zhang Yong , Jin Hongfang , Du Junbao 
Published: April 2022

BackgroundEarly identification of intravenous immunoglobulin (IVIG)-resistant Kawasaki disease (KD) is important for making a suitable therapeutic strategy for children with KD.MethodsThis study included a training set and an external validation set. The training set included 635 children (588 IVIG-sensitive and 47 IVIG-resistant KD) hospitalized in Wuhan Children’s Hospital, Hubei, China. Univariate analyses and binary logistic regression equation was incorporated to find the associated variables of the IVIG-resistant KD. A scoring model for predicting IVIG-resistant KD was established according to odds ratio (OR) values and receiver operating characteristic curves. The external validation set consisted of 391 children (358 IVIG-sensitive and 33 IVIG-resistant KD) hospitalized in Peking University First Hospital, Beijing, China. The predictive ability of the model of IVIG-resistant KD were externally validated by the real clinically diagnosed KD cases.ResultsFifteen variables in the training set were statistically different between IVIG-sensitive and IVIG-resistant KD children, including rash, duration of fever, peripheral blood neutrophil-to-lymphocyte ratio (NLR), prognostic nutritional index (PNI), percentage of monocytes and percentage of eosinophils, and serum alanine aminotransferase, aspartate aminotransferase, total bilirubin (TB), direct bilirubin, glutamyl transpeptidase, prealbumin, sodium ion, potassium ion and high-sensitivity C-reactive protein. According to logistic equation analysis, the final three independent correlates to IVIG-resistant KD were serum TB ≥ 12.8 μmol/L, peripheral blood NLR ≥ 5.0 and peripheral blood PNI ≤ 52.4. According to the OR values, three variables were assigned the points of 2, 2 and 1, respectively. When the score was ≥ 3 points, the sensitivity to predict IVIG-resistant KD was 80.9% and the specificity was 77.6%. In the validation set, the sensitivity, specificity and accuracy of the predictive model of IVIG-resistant KD were 72.7%, 84.9%, and 83.9%, respectively.ConclusionA scoring model was constructed to predict IVIG-resistant KD, which would greatly assist pediatricians in the early prediction of IVIG-resistant KD.
Small Human Population Drastic Impact, as Inferred From Multi-Proxies of a Temporary Carpathian Lake
Authors: Moldovan Oana Teodora , Miko Ladislav , Panaiotu Cristian , Roban Relu-Dumitru , Gąsiorowski Michał , Hercman Helena , Orza Răzvan , Kenesz Marius , Mirea Ionuț Cornel , Petculescu Alexandru , Robu Marius , Constantin Silviu 
Published: April 2022

Over the last thousands of years, human impact led to significant changes in the landscape, with impacts on the environment and the functioning of the ecosystems. We assumed that even small human settlements developed around water bodies might have a substantial impact that surpasses the natural cycles of climate change in an area, especially linked to forest cutting. We have chosen a temporary lake in the Romanian Carpathians as temporary lakes are overlooked in paleoclimatic studies, although they have potential as hotspots for paleoenvironmental research studies. Zăton Lake has exposed riverbank sediments dated with radioactive lead and optical stimulated luminescence techniques, analyzed for sediments and paleomagnetism, and sampled for fossil Oribatida mites. The results show that for the last 2,200 years, natural cycles of warm and cold periods changed the oribatid communities around the lake, with warmer cycles of rich fauna alternating with poor fauna during colder periods. However, in the last few centuries, the increased human occupation in the area replaced the ecologically diverse fauna with eurytopic taxa, more similar to the colder periods of the environmental evolution around the lake. Even if the human occupation around the lake was negligible, probably a few hundred to ∼2,500 people as in the present, the impact on the area is drastic, with ecologically nonspecific species becoming dominant. In conclusion, even small and temporary water bodies can contain unique and valuable information on past histories of climate change and human impacts. At regional scales, the combined effects of climate change and long-term human impacts in local areas can have deleterious effects on invertebrate species and communities.
Study on the Pore Structures and Adsorption Characteristics of Coking Coal of Liulin Mining in China Under the Condition of High Temperature and High Pressure
Authors: Lingling Qi , Xinshan Peng , Zhaofeng Wang , Xiangjun Chen , Juhua Dai 
Published: April 2022

In order to study the change of pore structure and adsorption characteristics of coking coal after the high-temperature and high-pressure adsorption test, the coking coal from the Liulin coalmine was selected for the research. Both the mercury injection experiments were carried out on the raw coal and the coal after the isothermal adsorption experiment processing with a pressure of 11 MPa and temperature ranging from 30 to 90°C. The results show that the pressure is beneficial to gas adsorption, while the temperature has a restraining effect on the gas adsorption of coking coal, and there is a good negative exponential relationship between the adsorption capacity and temperature. The hysteresis loop of that after the high-temperature and high-pressure isothermal adsorption test is smaller than that of raw coal, and the connectivity of pores becomes worse. In the process of the mercury injection experiment, the hysteresis loop of coking coal after the high-temperature and high-pressure adsorption experiment is smaller than that of raw coal. This demonstrates that the open pores decrease and the semi-closed pores increase, and then the connectivity of the pores becomes worse, which is not conducive to the gas flow when the coking is subjected to high-temperature and high-pressure action. After the high-temperature and high-pressure adsorption experiment, the volume of macropores, visible pores, and crannies of the coking coal decreases, and the volume of micropores and minipores increases. However, the total pore volume reduced overall. Under the same pressure, with the increase in temperature, the volume of macropores, visible pores, and crannies increases, while the volume of micropores and minipores decreases, and the total pore volume increases. After the high-temperature and high-pressure adsorption experiment, the proportion of micropores and minipores increases, and the specific surface area also increases. Under the same pressure, the surface areas of micropores and minipores decrease and the total specific surface area also decreases with the increase in temperature.
Parental Attitudes Toward Standard Newborn Screening and Newborn Genomic Sequencing: Findings From the BabySeq Study
Authors: Armstrong Brittan , Christensen Kurt D. , Genetti Casie A. , Parad Richard B. , Robinson Jill Oliver , Blout Zawatsky Carrie L. , Zettler Bethany , Beggs Alan H. , Holm Ingrid A. , Green Robert C. , McGuire Amy L. , Smith Hadley Stevens , Pereira Stacey ,   The BabySeq Project Team
Published: April 2022

Introduction: With increasing utility and decreasing cost of genomic sequencing, augmentation of standard newborn screening (NBS) programs with newborn genomic sequencing (nGS) has been proposed. Before nGS can be integrated into newborn screening, parents’ perspectives must be better understood.Objective: Using data from surveys administered to parents of healthy newborns who were enrolled in the BabySeq Project, a randomized clinical trial of nGS alongside NBS, this paper reports parents’ attitudes regarding population-based NBS and nGS assessed 3 months after results disclosure.Methods: Parental attitudes regarding whether all newborns should receive, and whether informed consent should be required for, NBS and nGS, as well as whether nGS should be mandated were assessed using 5-point scales from strongly disagree (=1) to strongly agree (=5). Parents’ interest in receiving types of results from nGS was assessed on a 5-point scale from not at all interested (=1) to very interested (=5). Survey responses were analyzed using Fisher’s exact tests, paired t-tests, and repeated measures ANOVA.Results: At 3 months post-disclosure, 248 parents of 174 healthy newborns submitted a survey. Support for every newborn receiving standard NBS (mean 4.67) was higher than that for every newborn receiving nGS (mean 3.60; p &lt; 0.001). Support for required informed consent for NBS (mean 3.44) was lower than that for nGS (mean 4.27, p &lt; 0.001). Parents’ attitudes toward NBS and nGS were not significantly associated with self-reported political orientation. If hypothetically receiving nGS outside of the BabySeq Project, most parents reported being very interested in receiving information on their baby’s risk of developing a disease in childhood that can be prevented, treated, or cured (86.8%) and their risk of developing a disease during adulthood that can be prevented, treated, or cured (84.6%).Discussion: Parents’ opinions are crucial to inform design and delivery of public health programs, as the success of the program hinges on parents’ trust and participation. To accommodate parents’ preferences without affecting the current high participation rates in NBS, an optional add-on consent to nGS in addition to NBS may be a feasible approach. Trial Registration ClinicalTrials.gov Identifier: NCT02422511.
CHSY3 can be a Poor Prognostic Biomarker and Mediates Immune Evasion in Stomach Adenocarcinoma
Authors: Li Xinwei , Fan Yongfei , Zhang Yue , Wang Yanyan , Zhao Menglin , Tang Mingyue , Li Huiyuan , Mi Jiaqi , Geng Zhijun , Wang Zishu , Su Fang 
Published: April 2022

Background: Chondroitin sulphate synthase 3 (CHSY3) is an important enzyme that regulates glycosylation, but it has not been reported in tumours. This study explored for the first time the oncological features of CHSY3 in stomach adenocarcinoma (STAD).Methods: We analysed CHSY3 expression in STAD through the Cancer Genome Atlas (TCGA) database and verified our findings by immunohistochemical staining and Western blot experiments. The prognostic value of CHSY3 in STAD was analysed through the biological aspects of CHSY3 in STAD, such as communal clinical follow-up survival data, methylation sites, tumour immune microenvironment (TIME) and immune cell surface checkpoints. Finally, the immune-evasion potential of CHSY3 in STAD was assessed on the Tumor Immune Dysfunction and Exclusion (TIDE) website and immunohistochemical staining experiment.Results:CHSY3 overexpression in STAD was associated with a poor prognosis based on immunohistochemical staining and Western blot experiments. Multivariate Cox analysis suggested that CHSY3 could be an independent prognostic risk factor. Pathway enrichment and TIME analysis demonstrated that CHSY3 up-regulated mesenchymal activation and immune activation signals in STAD, while TIDE assessment revealed that the risk of immune evasion was significantly higher in the high CHSY3 expression group than in the low CHSY3 expression group. Risk model scores based on CHSY3-associated immune cell surface checkpoints also presented poor prognosis, and immune evasion was significantly higher in the high-risk group than in the low-risk group.Conclusions: This study analysed CHSY3 from multiple biological perspectives and revealed that CHSY3 can be a biomarker of poor prognosis and mediates the TIME immune-evasion status in STAD.