bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mutational and bioinformatics analysis of the NKX2.1 gene in a cohort of Iranian pediatric patients with congenital hypothyroidism (CH)
Authors: Heidari Mohammad Mehdi , Madani Manshadi Seyed Ali , Eshghi Ahmad Reza , Talebi Fatemeh , Khatami Mehri , Bragança José , Ordooei Mahtab , Chamani Reyhane , Ghasemi Farzaneh 
Published: June 2022

Abstract
Congenital hypothyroidism (CH) occurs with a relatively alarming prevalence in infants, and if not diagnosed and treated in time, it can have devastating consequences for the development of the nervous system. CH is associated with genetic changes in several genes that encode transcription factors responsible for thyroid development, including mutations in the NK2 homeobox 1 (NKX2.1) gene, which encodes the thyroid transcription factor-1 (TTF-1). Although CH is frequently observed in pediatric populations, there is still a limited understanding of the genetic factors and molecular mechanisms contributing to this disease. The sequence of the NKX2.1 gene was investigated in 75 pediatric patients with CH by polymerase chain reaction (PCR), single-stranded conformation polymorphism (SSCP), and direct DNA sequencing. Four missense heterozygous variations were identified in exon 3 of the NKX2.1 gene, including three novel missense variations, namely c.708A>G, p.Gln202Arg; c.713T>G, p.Tyr204Asp; c.833T>G, p.Tyr244Asp, and a previously reported variant rs781133468 (c.772C>G, p.His223Gln). Importantly, these variations occur in highly conserved residues of the TTF-1 DNA-binding domain and were predicted by bioinformatics analysis to alter the protein structure, with a probable alteration in the protein function. These results indicate that nucleotide changes in the NKX2.1 gene may contribute to CH pathogenesis.
The epidemiological impact of the Canadian COVID Alert app
Authors: Sun Shuo , Shaw Mairead , Moodie Erica E.M. , Ruths Derek 
Published: June 2022

AbstractObjectivesWe analyzed the effectiveness of the Canadian COVID Alert app on reducing COVID-19 infections and deaths due to the COVID-19 virus.MethodsTwo separate but complementary approaches were taken. First, we undertook a comparative study to assess how the adoption and usage of the COVID Alert app compared to those of similar apps deployed in other regions. Next, we used data from the COVID Alert server and a range of plausible parameter values to estimate the numbers of infections and deaths averted in Canada using a model that combines information on number of notifications, secondary attack rate, expected fraction of transmissions that could be prevented, quarantine effectiveness, and expected size of the full transmission chain in the absence of exposure notification.ResultsThe comparative analysis revealed that the COVID Alert app had among the lowest adoption levels among apps that reported usage. Our model indicates that use of the COVID Alert app averted between 6284 and 10,894 infections across the six Canadian provinces where app usage was highest during the March–July 2021 period. This range is equivalent to 1.6–2.9% of the total recorded infections across Canada in that time. Using province-specific case fatality rates, 57–101 deaths were averted during the same period. The number of cases and deaths averted was greatest in Ontario, whereas the proportion of cases and deaths averted was greatest in Newfoundland and Labrador. App impact measures were reported so rarely and so inconsistently by other regions that the relative assessment of impact is inconclusive.ConclusionWhile the nationwide rates are low, provinces with widespread adoption of the app showed high ratios of averted cases and deaths (upper bound was greater than 60% of averted cases). This finding suggests that the COVID Alert app, when adopted at sufficient levels, can be an effective public health tool for combatting a pandemic such as COVID-19.
MELAMPAUI NARASI STIGMATISASI IDENTITAS PEREMPUAN PENGGEMAR: BTS ARMY
Authors: Vasthia Devi Jasmine Floretta 
Published: June 2022

<p><strong>Decades have passed but female fans who love male idols are still stigmatized, judged, and reduced to their existence in society and popular culture. This is what also happened to ARMY, fans of the male idol group BTS. Using a literature review elaborated through a feminist perspective, this article will discuss how ARMY is stigmatized not only because of the perceived inferiority of women and the domination of men in society, but also because of their love for male groups including male groups. subordinated within the circle of hegemonic masculinity. However, the gender stigma and stereotype attached to them was successfully broken. ARMY as a female fan group realizes that in essence humans are free creatures who must always make choices in life that will shape them as authentic individuals. With this full awareness, they took action through fan labor and fan activism practices, which in the end led them to optimize their Creative Self.</strong></p><p><strong> </strong></p><p><strong>Keywords: ARMY; fan labor; fan activism; Creative Self; authentic</strong></p><p><strong> </strong></p><p><strong>Sudah beberapa dekade berlalu namun perempuan penggemar yang menggemari idola laki-laki masih terstigmatisasi, dihakimi, dan direduksi eksistensinya di tengah masyarakat dan budaya populer. Hal inilah yang juga terjadi pada ARMY, penggemar dari group idola laki-laki BTS. Dengan menggunakan kajian literatur yang dielaborasi melalui perspektif feminis, artikel ini akan membahas bagaimana ARMY distigma bukan hanya karena adanya anggapan inferioritas perempuan dan adanya dominasi laki-laki di dalam masyarakat, namun juga karena kecintaan mereka terhadap group laki-laki yang termasuk kelompok laki-laki tersubordinasi di dalam lingkar hegemoni maskulinitas. Namun stigma dan steriotipe gender yang dilekatkan pada mereka berhasil dipatahkan. ARMY selaku kelompok perempuan penggemar menyadari bahwa pada hakikatnya manusia adalah makhluk yang bebas yang harus selalu memilih pilihan-pilihan dalam hidupnya yang akan membentuknya sebagai individu autentik. Dengan kesadaran penuh ini mereka melakukan aksi melalui praktik <em>fan labor </em>dan <em>fan activism</em> yang mana pada akhirnya menghantarkan mereka pada pengoptimalan Diri Kreatif.</strong></p><p><strong> </strong></p><p><strong>Kata kunci: ARMY; fan labor; fan activism; Diri Kreatif; autentik</strong></p>
Strategi Adaptasi Komunitas Sedulur Sikep Desa Klopodhuwur Blora di Era Globalisasi
Authors: Dyani Nurmalya Ayu , Yuhastina Yuhastina Yuhastina , Nurhadi Nurhadi Nurhadi 
Published: June 2022

<p>In the era of globalization which was characterized by the advancement of science and technology, caused changes in several aspects of life in the sedulur sikep community in Klopodhuwur village. These changes, also cause various challenges for local confidence convenience such as sedulur sikep communities. Therefore, the purpose of this study is to identify the challenges faced by sedulur sikep communities in the era of globalization and explain the adaptation strategies they do. This study uses qualitative research methods with the presentation of writing is descriptive analysis. The results of this study indicate that the challenges experienced by the Samin Blora community include: 1) the religious education system, 2) government as a change actor, 3) negative stigma from the outside community, and 4) technology development in agriculture. In order to maintain its existence, the Sedulur Sikep Community of Klopodhuwur Village has several forms of adaptation strategies, namely 1) adoption of religious elements, 2) internalization of ajaran Sedulur Sikep, 3) Formation of customary institutions, 4) interactions with outside society, and 5) technology limitations.</p><p> </p><p>Di era globalisasi yang ditandai dengan kemajuan ilmu pengetahuan dan teknologi, menyebabkan terjadinya perubahan dalam beberapa aspek kehidupan pada komunitas sedulur sikep Desa Klopodhuwur Blora. Perubahan-perubahan tersebut, juga menyebabkan adanya berbagai tantangan bagi penghayat kepercayaan lokal seperti komunitas sedulur sikep. Oleh sebab itu, tujuan dari penelitian ini adalah untuk mengidentifikasikan tantangan-tantangan yang dihadapi oleh komunitas sedulur sikep di era globalisasi dan menjelaskan strategi adaptasi yang dilakukannya. Penelitian ini mengunakan metode penelitian kualitatif dengan penyajian tulisan bersifat deskriptif analisis. Hasil penelitian ini menunjukkan bahwa tantangan yang dialami oleh masyarakat samin Blora antara lain : 1) sistem pendidikan agama, 2) pemerintah sebagai aktor perubahan, 3) stigma negatif dari masyarakat luar, dan 4) perkembangan teknologi di bidang pertanian. Agar tetap dapat mempertahankan eksistensinya, komunitas sedulur sikep Desa Klopodhuwur mempunyai beberapa bentuk strategi adaptasi yaitu 1) adopsi unsur agama, 2) internalisasi ajaran sedulur sikep, 3) pembentukan lembaga adat, 4) interaksi dengan masyarakat luar, dan 5) limitasi teknologi.<strong></strong></p>
Pyrexia of unknown origin: A microbiological review of cases in pediatric population at a tertiary care hospital
Authors: Bowalekar Sneha S. , Vijaykumar Vijitha 
Published: June 2022

: Pyrexia of unknown origin (PUO) was described as a persistent fever above 38.3°C (100°F) that evades diagnosis for at least 3 weeks, including 1 week of investigation in hospital. It may be caused by over numerous infectious, rheumatic/ inflammatory, malignant/neoplastic and miscellaneous disorders.: Blood specimen of 55 pediatric patients with fever of more than two weeks duration were tested for Typhoid, Rickettsial and Brucella infection serologically. Blood cultures of these patients were tested simultaneously. : Positivity of Immunochromatographic testing for S. Typhi, Widal slide and tube agglutination test was 20%, 23.6% and 21.8% respectively. Titres of &#62;=1:320 were observed in 4 (33.3%) of widal positive patients against H agglutinins. Positivity in Weil-Felix Tube Agglutination test was observed in 1(1.8%) patient against OX-K antigen, in 8 (14.5%) patients against OX-2 and in 6 (10.9%) against OX-19 antigen. Titre of 1:640 was observed in 1(1.82%) of patients against OX-2. One (1.8%) patient showed positive result in Brucella A/M Tube agglutination test.: Although serological investigations provide early and presumptive diagnosis of diseases, clinical features and epidemiological factors play an important role for initiation of treatment where facilities for culture and molecular testing are lacking or the results of the same are negative.
Occurrence of Pseudomonas aeruginosa isolated from surgical site infections and wound infections and their antimicrobial susceptibility profile
Authors: Sharma Shweta R , Singh Rinki Kumari , Singh Asok Kr , Farooq Umar , Singh Sudhir , Sharma Vasundhara , Ahamad Imran 
Published: June 2022

is a major cause of health care associated infections. It has natural resistance as well as acquired multidrug resistance to various antibiotics leading to increased morbidity and mortality, which lead to problematic condition and increased medical expenses. The main aim of this study was to study the resistance pattern of isolated from samples received from surgical site infections and wound infections.This study was conducted at Microbiology laboratory of Teerthanker Mahaveer Medical College and Research Centre. Pus and wound swab samples were collected from surgical site infections (SSIs) and wound infections. Samples were cultured on MacConkey and Nutrient agar and incubated at 37°C aerobically. Identification of was done by using conventional method and antibiotic sensitivity was determined with automated Vitek2 compact system.From January 2021 to November 2021 during this study period, out of 117 positive samples 32 (27.35%) were isolated. The susceptibility pattern showed the organism to be most commonly susceptible to Colistin, Doripenem, followed by Piperacillin/Tazobactam, Imipenem, Amikacin, and was less susceptible to Ciprofloxacin, Ceftazidime.Presences of multidrug resistance strains have raised significant concern and judicious use of antibiotics is the only option to reduce drug resistance and preventing resistance against reserve antibiotics.
Bacterial isolates & their resistance patterns from blood cultures,KIMS
Authors: Padmaja N , .V Srilalitha , Sree K.Ramya 
Published: June 2022

Bloodstream infections (BSI) remain one of the important causes of morbidity and mortality. Microorganisms present in circulating blood result in serious sequelae like shock, DIC, and death. Blood culture is a vital tool for their detection. Drug sensitivity patterns help in rationalizing the treatment. The present study was undertaken to determine the bacterial profile & resistance patterns in our hospital.To study the bacterial isolates and their resistance patterns from blood cultures.A retrospective study was conducted from August 2019 to January 2020 in the Dept. of Microbiology, KIMS, Amalapuram. Blood samples were collected from clinically suspected patients with aseptic techniques, processed, isolated and identified according to standard microbiological techniques. Antibiotic sensitivity testing was done according to Kirby-Bauer disk diffusion method.A total of 57 pathogens were isolated from 380 suspected patients of BSI. Gram-negative bacilli (52.7%) (GNB) were predominant organisms isolated followed by Gram-positive cocci (47.3%) (GPC).(20) and pneumoniae (19) were the predominant pathogens isolated. Among GPC, was highly resistant to Penicillin (85%), Ampicillin (85%), Amoxyclav (75%), and Ciprofloxacin (50%). All Staphylococcal species were sensitive to Methicillin, Vancomycin, Linezolid. Among GNB, exhibited high resistance to Cefixime (95%), Amoxyclav (84%), Ceftazidime (79%). Among 30 GNB, 12(40%) were ESBL producers. Most of the GNB were sensitive to Imipenem.Appropriate treatment of BSI should be based on the current knowledge of local bacterial resistance patterns of the hospital. Hence this kind of study will help in formulating management guidelines and antibiotic policy
Burden of Klebsiella pneumoniae in late onset neonatal sepsis in a tertiary care hospital in Western Maharashtra
Authors: Karyakarte Rajesh , Sagar Drishti , Pol Sae 
Published: June 2022

is a notorious superbug that is rapidly becoming the most common nosocomial pathogen to be isolated from intensive care settings. : To determine the burden of and associated risk factors of late onset sepsis among neonates admitted into neonatal intensive care unit (NICU).: A prospective study was conducted among 200 neonates admitted into the NICU of Sassoon General Hospital from January 1, 2019 to December 31, 2019. Neonates &#60;28 days of age exhibiting clinical signs and symptoms of sepsis &#62;72hrs after birth were included in the study. A detailed case history format of patient information was prepared. Blood sample was subjected to identification by culture and real time polymerase chain reaction (RT-PCR). Neonates who were positive by blood culture were confirmed as having sepsis.: Frequency of was 33.3% by blood culture and 38.1% by RT-PCR. Most of the septic neonates were males (n=6; 60%), formula fed (n=7; 70%), extremely low birth weight (n=6; 60%), delivered through caesarean section (n=6, 60%) and were preterm (n=7; 70%). Mortality was seen in 2 (20%) cases. All the isolates were sensitive to meropenem, piperacillin + tazobactam, amikacin, polymyxin B and colistin. : Occurrence of was 33.3% in late onset neonatal sepsis by blood culture. Low birth weight, formula feeding, prematurity and caesarean section were associated with a higher risk of sepsis in these patients. Antimicrobial stewardship and hospital infection control measures are need of the hour in preventing late onset neonatal sepsis.
The Steroid Hormone Profile in Etawah Crossbreed Goat While Ovulation Induced using The Selectsynch Method
Authors: Aftabuddin Rz Muhammad , Srianto Pudji , Nidom Chairul Anwar , Madyawati Sri Pantja , Sardjito Trilas , Ismudiono Ismudiono 
Published: June 2022

This study aimed to provide alternative information and solutions in an effort to increase reproductive productivity in etawah crossbreed goats (PE). The sample used in this study was 10 female PE with an average age of 2.5 - 3 years and primiparous at least . Ovulation induction was performed using 0.1 mg intra-muscular Gonadotropin-Releasing Hormone (GnRH), after seven days injection of Prostaglandin F2α (PGF2α) was given as much as 2.5 mg submucosa of the vulva, followed by a second injection of GnRH as much as 0,2 mg intramuscular in samples that have really experienced heat and selected selectively according to the signs of natural heat shown by the sample and followed by insemination as much as 2 doses or 0.50 ml of frozen semen. Blood sampling was performed at H0, H7th, H14th and H21th after insemination. All blood samples were collected and progesterone and estrogen hormone profiles were examined using the ELISA method. From the results of the ELISA test, the mean progesterone hormone profile H0 = 4.798 ng / ml, H7th = 4.887 ng / ml, H14th = 4.824 ng / ml, H21th = 5.148 ng / ml. The profile of the hormone estrogen at H0 = 19,461 pg / ml, H7th = 17,457 pg / ml, H14th = 18,248 pg / ml, H21th = 17,515 pg / ml. This study showed an increase in the levels of the progesterone hormone at H0 to H7th, then slightly decreased in H14th and a significant increase in H21th. For the estrogen hormone, there is a decrease from H0 to H7th then there is a slight increase in H14th and decreases again in H21th.
Maternidade e aposentadoria no RGPS: como as mulheres com filhos são afetadas pela reforma previdenciária da Emenda Constitucional 103/2019?
Authors: Wiederkehr Bianca , Afonso Luís Eduardo 
Published: June 2022

Este trabalho analisa como a aposentadoria pode ser impactada pela maternidade, dado que pode afetar os fluxos de renda, contribuições e benefícios. Emprega-se um modelo atuarial com densidades contributivas diferenciadas por nível de renda. Como o número de contribuições varia em função destas densidades, a idade de aposentadoria é calculada de forma endógena, com a realização de um conjunto de simulações. Foram calculados cinco indicadores previdenciários em diferentes cenários, dados por combinações do evento da maternidade, idade no nascimento do filho, duração do afastamento do mercado de trabalho e salário no retorno ao mercado de trabalho. Os cálculos foram feitos para a regra antiga do Regime Geral de Previdência Social (RGPS), que vigorou até 2019, e para a regra nova, que passou a valer em 2020, após a aprovação da Emenda Constitucional n. 103/2019. Observou-se queda no caráter progressivo das aposentadorias do RGPS devido à reforma de 2019, bem como redução na maioria dos indicadores, particularmente na taxa interna de retorno. Mas a taxa de reposição pode aumentar para alguns grupos devido à extensão do período contributivo. Trabalhadoras sem filhos e aquelas que não deixam o mercado de trabalho devido à maternidade são afetadas de maneira razoavelmente similar pela reforma. Trabalhadoras que precisam deixar o mercado de trabalho são mais afetadas, com redução nos indicadores que dependem do período de recebimento do benefício de aposentadoria.
The Right to a Healthy Environment in the Hungarian Constitution
Authors: Paulovics Anita , Jámbor Adrienn 
Published: June 2022

The aim of the study is to present the development of the environmental law institutions founded up to the present day. Regulations concerning the protection of the environment had first been defined on international level before they appeared in the national legal system. Basic questions of environmental law are being analysed in this study. The history of environmental law is reviewed briefly from the 1950s to the present day both in international and national aspects as well as the constitutional foundation of environment protection within the right to a healthy environment and its Constitutional Court practice. This study will not touch upon the detailed study of the underlying principals of the national environmental law.
Numerical Simulation of Proppant Transport and Placement in Hydraulic Fractures with the Hybrid Perkins-Kern-Nordgren-Carter (PKN-C) Model and Particle Tracking Algorithm
Authors: Ding Yanan , Yang Daoyong , Huang Hai , Wang Haiwen 
Published: June 2022

Summary
Although non-Newtonian fracturing fluids have been widely used, numerical simulation of field-scale proppant transport considering non-Newtonian fracturing fluids is far from satisfactory. In this study, a novel numerical scheme based on the Eulerian-Lagrangian (E-L) method has been developed and validated to simulate such a proppant transport and placement behavior. More specifically, hydraulic fracture propagation is characterized by the Perkins-Kern-Nordgren-Carter (PKN-C) model, and the injected proppants are described using the classic particle tracking algorithm. Proppants are vertically dragged by the gravitational force and horizontally driven by the velocity field conditioned to the fracture propagation and proppant dune packing. The settling velocity of proppants is quantified considering the in-situ shear rate and concentration, while their transport at each dune surface is quantified by performing drag/lift force analysis. The numerical model is first validated by reproducing experimental measurements inside a visual parallel plate. Subsequently, field-scale simulations are performed to identify the factors dominating proppant transport and placement under various conditions. As indicated by simulated results, the accumulated concentration at the lower region of a fracture usually results in a growing proppant dune with a “heel-biased” distribution. The non-Newtonian fluid yields a higher slurry coverage together with a longer proppant dune than the Newtonian fluid when their average viscosities are consistent. In addition to the dependence of the premature tip screenout configuration on the power-law fluid parameter n, both parameters of K and n impose a generally consistent effect (on proppant transport) with that of Newtonian viscosity (i.e., an increase of either K or n effectively improves the average viscosity and mitigates the proppant settling). A mild increase in proppant density and size significantly enhances the proppant dune formation; however, a further increase of these two factors aggravates the “heel-biased” distribution of proppants. Also, an increased leakoff coefficient improves the overall proppant concentration as well as the dune and slurry coverage. The used particle tracking algorithm enables proppant transport to be individually and accurately evaluated and analyzed with an acceptable computational cost, while such a numerical model can deal with both the Newtonian and non-Newtonian fluids at the field scale. This numerical study allows us to optimize the growth, propagation, and coverage of proppant dunes for maximizing fracture conductivity during hydraulic fracturing operations.