bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coupling strategy between high-index and mid-index micro-metric waveguides for O-band applications
Authors: Skandalos Ilias , Bucio Thalia Dominguez , Mastronardi Lorenzo , Rutirawut Teerapat , Gardes Frederic Y. 
Published: July 2022

Abstract
The integration of fast and power efficient electro-absorption modulators on silicon is of utmost importance for a wide range of applications. To date, Franz Keldysh modulators formed of bulk Ge or GeSi have been widely adopted due to the simplicity of integration required by the modulation scheme. Nevertheless, to obtain operation for a wider range of wavelengths (O to C band) a thick stack of Ge/GeSi layers forming quantum wells is required, leading to a dramatic increase in the complexity linked to sub-micron waveguide coupling. In this work, we present a proof-of-concept integration between micro-metric waveguides, through the butt-coupling of a 1.25 μm thick N-rich silicon nitride (SiN) waveguide with a 1.25 μm thick silicon waveguide for O-band operation. A numerical analysis is conducted for the design of the waveguide-to-waveguide interface, with the aim to minimize the power coupling loss and back-reflection levels. The theoretical results are compared to the measured data, demonstrating a coupling loss level of 0.5 dB for TE and TM polarisation. Based on the SiN-SOI interconnection, a theoretical analysis of a quantum-confined Stark effect (QCSE) stack waveguide coupled to an SiN waveguide is then proposed.
An Efficient Method to Compute the Stationary Probabilities of the GIX/M/c/N Model
Authors: Taha Muhammad El , Michaud Thomas 
Published: July 2022

Abstract
Consider the batch-arrival GIX/M/c/N model with c servers, general inter-arrival batch times, finite buffer, and exponential service times. Inter-arrival batch times, batch sizes, and service times are i.i.d. and independent of each other. In this article we give a simple efficient algorithm to derive an exact solution for the steady state system size probabilities. The starting point is computing the one-step transition probabilities of the imbedded Markov chain observed at the system arrival epochs of the the corresponding G/M/c model. The one-step transition probabilities are computed exactly by converting a numerical integration problem into a finite sum. Another key contribution is generating the transition probabilities of the batch-arrival model by using a simple and intuitive method to extend the results of the standard GI/M/c model to batch arrivals with and without a finite buffer, and in the case of finite buffer with partial and full batch rejection. Moreover, we develop an efficient stable algorithm that can accurately solve problems with a larger number of servers than previously known. We give numerical examples to demonstrate the performance of our method.
Lnc NR2F1-AS1 promotes breast cancer metastasis by targeting miR-25-3p/ZEB2 axis
Authors: Zhou Yu , Zhai Duanyang , Kuang Xiaying , Shao Fangyuan , Zhen Tiantian , Lin Ying , Wang Qing , Shao Nan 
Published: July 2022

Abstract
Background: Long noncoding RNAs (LncRNAs) has been demonstrated to have a considerable effect on tumor metastasis and are aberrently expressed in a variety of cancers. Nevertheless, its role in breast cancer (BC) remains incompletely inventoried.Methods: The microarray assay of differentially expressed lncRNAs in epithelial-mesenchymal transition (EMT) and non-EMT cells was performed. The prognostic value of lnc NR2F1-AS1 expression in BC patients were analyzed in the TCGA database. Lnc NR2F1-AS1 expression level in different BC cell line were assessed by qPCR. The role of lnc NR2F1-AS1 in BC cell metastasis was investigated in vitro and in vivo. Dual luciferase reporter assay and RNA immunoprecipitation (RIP) were performed to uncover the relationship between lnc NR2F1-AS1, miR-25-3p, and ZEB2. Results: High level of lnc NR2F1-AS1 was observed in BC cells undergoing EMT and was clolsely correlated with adverse prognosis in BC patients. Lnc NR2F1-AS1 knockdown notably inhibited BC cell migration, invasiveness in vitro and metastasis in vivo. Mechanistically, lnc NR2F1-AS1 competitively bind with miR-25-3p to impede the degradation of ZEB2, which is a positive EMT transcription factor in BC. Conclusions: Taken together, our study reveals a novel lnc NR2F1-AS1/miR-25-3p/ZEB2 axis for BC metastasis and indicate that lnc NR2F1-AS1 may serve as a potential therapeutic target for breast cancer metastasis.
Comparative antibacterial analysis of the anthraquinone compounds based on the AIM theory, molecular docking and dynamics simulation analysis
Authors: Qi Yanjiao , Wang Mingyang , Zhang Bo , Liu Yue , Song Li , Jin Nengzhi , Zhang Hong 
Published: July 2022

Abstract
Hydroxyanthraquinones and anthraquinone glucoside derivatives are always considered as the active antibacterial components. Comprehensive comparison and analysis of these compounds were performed for their structure characteristics and antibacterial effect by applying quantum chemical calculations, atoms in molecules theory, molecular docking and dynamics simulation procedure. The molecular geometric configuration, stability, electrostatic potential, the frontier orbital energies and topological properties were analyzed. Once glucose ring is introduced into the hydroxyanthraquinone rings, almost all of the positive molecular potentials are distributed among the hydroxyl hydrogen atoms of the glucose rings. In addition, low electron density ρ (r) and positive Laplacian value of the O-H bond of the anthraquinone glucoside are the evidences of the highly polarized and covalently decreased bonding interactions. The anthraquinone glucoside compounds have generally higher intermolecular binding energies than the corresponding aglycones due to the strong interaction between the glucose rings and the surrounding amino acids. Molecular dynamics simulations further explored the stability and dynamic behavior of the anthraquinone compound and protein complexes through RMSD, RMSF, SASA and Rg. The type of carboxyl, hydroxyl, hydroxylmethyl groups on phenyl ring and the substituent glucose rings are important to the interactions with the topoisomerase type II enzyme DNA gyrase B.
The Relationship between Academic Stress and Non-Suicidal Self-Injury Behavior among Adolescents in Ethnic Areas: The Moderating Effect of Self-efficacy and Parental Supports
Authors: LAN Zhensong , Pau Kee , Yu Hapsah Md , Huang Xuefang , Xu Ruiying 
Published: July 2022

Abstract
Background
Due to China's large population and uneven distribution of educational resources, teenagers are faced with various competitions from birth, coupled with the importance parents attach to education in Chinese culture, which undoubtedly makes teenagers bear more pressure. Academic stress has always been one of the main pressures faced by Chinese adolescents, and there is a strong link between academic stress and non-suicidal self-injury (NSSI) behavior of Chinese adolescents.
Methods
a cross-sectional study was conducted in 10 randomly selected middle schools in Guangxi Province, China to examine the relationship between academic stress and adolescents' NSSI behavior, and to further analyze the relationship between adolescents' self-efficacy and parental support in stress and NSSI. A total of 2344 children were included in the analysis, including 1142 boys (48.72%) and 1202 girls (51.28%). Descriptive statistics will be used to understand the levels of adolescents' NSSI, academic stress, self-efficacy, and parental support; T-tests will be used to compare differences in adolescents' NSSI across demographic characteristics, academic achievement, and family economic status. A multiple linear regression model was used to examine the interaction between academic stress and NSSI in terms of adolescents' self-efficacy and parental support.
Results
The prevalence of NSSI was not significant among adolescents of different ethnic groups (t=-1.48, p = 0.139), but academic stress had a positive effect on adolescents' NSSI behavior (R = -0. 246, p < 0.01). Self-efficacy and parental support can moderate the relationship between academic stress and NSSI behavior, and the moderating effect of parental support is stronger than that of self-efficacy; the interaction of self-efficacy and parental support has a negative effect on the relationship between adolescent stress and NSSI behavior, but for adolescents with high level of self-efficacy, the more parental support, the more likely it is to cause adolescent NSSI behavior.
Conclusion
Academic stress has a negative effect on adolescents' NSSI behavior, and self-efficacy and parental support can alleviate the negative effect of adolescent stress on NSSI behavior. However, it is necessary to provide appropriate parental support for adolescents with different levels of self-efficacy.
How does long-term artificial intervention select the microbial community? A case study of the cellar mud chronosequence in the Chinese liquor brewing ecosystem
Authors: Zhong Xiaozhong , Chai Lijuan , Zeng Yan , Zhang Xiaojuan , Lu Zhenming , Liu Guangqian , Tu Tingyao , Lu Lingfei , Zhang Rui , Yu Hui , Zhang Suyi , Wang Songtao , Shen CaiHong , Shi Jinsong , Xu Zhenghong 
Published: July 2022

Abstract
Background: Despite artificial selection being proposed to engineer microbiome from the top down to obtain the desired practical phenotypes, we lack the empirical evidence regarding the feasibility of the community-level selection. Chinese liquor fermentation for centuries caused sustained shifts of the diversity and structure of the microbial community within the cellar mud, thus providing an ideal ecosystem to study the microbiome evolution under long-term artificial selection. While multiple previous studies have reported the microbial diversity of cellar mud microbiome, few have resolved the metabolic roles of the microbial community and their relationships with cellar mud aging. Results: We used genome-centric metagenomics to analyze the microbial community of cellar mud samples (n=120) for four ages spanning 100 years. Our results showed that cellar mud microbiota was dominated by Bacteroidota, Firmicutes, and Halobacteriota lineages, with the discovery of diverse novel species. Metabolic reconstruction of these microorganisms indicated that macromolecules hydrolysis, fatty acid chain elongation, syntrophic fatty acid oxidation, and methanogenesis pathways were core functions of the cellar mud microbial ecosystem. During cellar mud initiation, we identified Bacteroidota with high hydrolysis capability as the dominant community members. Contrastingly, the proportion of Bacteroidota in mature and deep aging cellar mud decreased, while that of Firmicutes with potential of syntrophic fatty acids oxidation and methanogens increased. Metabolic potential of fatty acid chain elongation was stable throughout the development of cellar mud, with taxonomic variation being observed against environmental change. Conclusions: This study unravels the structure, function, and succession of microbial communities inhabiting the cellar mud, with changing metabolic cross-feeding interactions for environmental adaption. Our findings provide fundamental and comprehensive knowledge for exploring the impact of artificial ecosystem selection on the microbial community.
Single-Cell Analysis of Cell-Type Specific Immune Response in Pneumococcal Meningitis
Authors: Zhang Yujie , Duan Jing , Lin Sufang , Wen Jialun , Liao Jianxiang 
Published: July 2022

Abstract
Streptococcus pneumoniae is a Gram-positive, human-specific commensal infectious pathogen that can lead to death worldwide, especially in children under five. A deeper understanding of the immune response in pneumococcal meningitis (PM) has high scientific importance. In this study, we performed single-cell RNA sequencing of peripheral blood mononuclear cells from PM patients and healthy individuals. Both platelets and neutrophils were found with higher proportions in the PM group compared to the control group. Of the differentially expressed genes (DEGs), the genes involved in the immune functions of neutrophils were up-regulated in many immune cells, including platelets, myeloid cells and B cells, suggesting excessive neutrophil activation; while the genes involved in antigen processing and presentation in myeloid cells and B cells were down-regulated in the PM group, which indicated adaptive immune deficiency in the PM patients. Consistently, the proportions of plasmacytoid dendritic cells and T cells were decreased. Furthermore, genes involved in cytotoxity were down-regulated in both NK cells and T cells, and T cell activation was deficient. Many DEGs were also enriched in some signaling pathways that mediate interactions between S. pneumoniae and immune cells, including the Toll-like receptor pathway, the Nucleotide oligomerization domain-like signaling pathway, and the tumor necrosis factor signaling pathway. In conclusion, our study provided a global characterization of S. pneumoniae-induced systemic immune responses, which may have implications for identification of potential targets to improve the immune response in PM.
New record of brachyuran crabs from the Visakhapatnam coast, Andhra Pradesh
Authors: Kumar J. S. Yogesh , Mahapatra Parnasree , Sen Arya , Panda Pradip 
Published: July 2022

Abstract
Two species of brachyuran crabs from two families, Eucrate indica Castro & Ng, 2010 belonging to the family Euryplacidae and another one is Liagore rubromaculata (De Haan, 1983) belonging to the family Xanthidae are reported for the first time from the Visakhapatnam coast, Andhra Pradesh. The crabs were collected from the Visakhapatnam fishing harbour in the trawl bycatch during the reef and reef-associated faunal survey along Andhra coast. The morphological features, taxonomical systematic, colour and distribution of these crabs are discussed herein.
Axonal Spectrum Imaging (AxSI): In-Vivo mapping of axonal diameter distributions in the human brain
Authors: Gast Hila , Horowitz Assaf , Krupnik Ronnie , Barazany Daniel , Lifshits Shlomi , Ben-Amitay Shani , Assaf Yaniv 
Published: July 2022

Abstract
In this paper we demonstrate a generalized and simplified pipeline called axonal spectrum imaging (AxSI) for in-vivo estimation of axonal characteristics in the human brain. Whole-brain estimation of the axon diameter, in-vivo and non-invasively, across all fiber systems will allow exploring uncharted aspect of brain structure and function relations. While axon diameter mapping is important in and of itself, its correlation with conduction velocity will allow, for the first time, the explorations of information transfer mechanisms within the brain. We demonstrate various well-known aspects of axonal morphometry (e.g., the corpus callosum axon diameter variation) as well as other aspects that are less explored (e.g., axon diameter-based separation of the superior longitudinal fascicules into segments). Moreover, we have created an MNI based mean axon diameter maps over the entire brain for a large cohort of subjects providing the reference basis for future studies exploring relation between axon properties, brain function, physiology and behavior.
Outcomes of the crushing and ligation technique for pancreatic dissection: A cohort study
Authors: Abe Kyohei , Okamoto Tomoyoshi , Matsumoto Michinori , Futagawa Yasuro , Yanaga Katsuhiko 
Published: July 2022

Abstract
Background
Prevention and treatment of postoperative pancreatic fistulas is a crucial problem associated with pancreaticoduodenectomies and distal pancreatectomies. The objective of this study was to determine the outcomes of the “crushing and ligation” technique for liver dissection during pancreaticoduodenectomy and distal pancreatectomy procedures.
Methods
We evaluated the outcomes of 44 patients who underwent pancreatic dissections using the crushing and ligation technique and 44 patients who underwent the ultrasonically activated scalpel technique. Operative duration, blood loss, length of hospital stay, incidence of postoperative pancreatic fistulas (grade BC), and postoperative drainage amylase fluid level were compared between groups.
Results
Postoperative pancreatic fistulas (grade BC) occurred in 2 (8.0%) and 13 (44.8%) patients in the pancreaticoduodenectomy-crushing and ligation and pancreaticoduodenectomy-ultrasound groups, respectively (p = 0.0026). The pancreaticoduodenectomy-crushing and ligation group had significantly lower maximum amylase levels in drainage fluid (p = 0.0387) and significantly longer operative durations (p = 0.0422), but a shorter postoperative length of hospital stay (p = 0.0159), than the pancreaticoduodenectomy-ultrasound group. Postoperative pancreatic fistulas (grade BC) occurred in 2 (10.5%) patients in the distal pancreatectomy-crushing and ligation group, which was significantly lower than in the distal pancreatectomy-ultrasound group (7 patients, 46.7%).
Conclusion
Crushing and ligation may lead to better outcomes and fewer complications than the conventional ultrasonically activated scalpel technique.
Rab11A depletion in microglia-derived extracellular vesicle proteome upon beta-amyloid treatment
Authors: Mignogna Giuseppina , Fabrizi Cinzia , Correani Virginia , Giorgi Alessandra , Maras Bruno 
Published: July 2022

Abstract
Microglia, the macrophage-like glial cells, behave as sentinels against exogenous pathogens invading the neural tissue. Their commitment is not only confined to the defensive function, but they also perform balancing trophic activities such as neuronal postnatal development, remodelling and pruning of synapses. Likewise, microglia-derived extracellular vesicles (EVs) can play strategic roles in maintaining health brain by modulating neuronal activity and by controlling neurite outgrowth as well as innate immune response. Nevertheless, strong evidence also points to their role in the development of neurodegenerative pathologies such as Alzheimer’s disease (AD). Here, we explored EV protein content released by BV2 microglial cells in resting state and after stimulation with beta-amyloid peptides (Ab), mimicking conditions occurring in AD. In the resting BV2 cells, we extended the list of proteins present in mouse microglia EV cargo with respect to those reported in Vesiclepedia exosome database while, in amyloid-triggered microglia, we highlighted a pronounced drop in EV protein content. Focusing on Rab11A, a key factor in the recycling routes of amyloid species, we observed a dramatic decrease of this protein in Aβ-treated microglia EV cargo with respect to the EVs from the untreated sample. This decrease might affect the delivery of Rab11A to neurons thus increasing the harmful amyloid burden in neuronal cells that eventually may lead to their death. We tentatively proposed that alterations observed in EVs derived from Aβ-treated microglia may represent molecular features that, among other, shape the disease-associated microglia phenotype, a recently proposed subset of microglial population, present in neurodegenerative pathologies.
Glucose withdrawal-induced Chinese hamster ovary cells apoptosis and its potential role in process robustness assessment
Authors: Guo Meijin , Xiao Shang , Li Qiang , Jiang Jinlong , Huo Chengxiao , Chen Hao 
Published: July 2022

Abstract
Establishing a robust process has always been one of the main goals of cell culture process development. To achieve this goal, glucose needs to be maintained at a low concentration to reduce lactate production, the main metabolic by-product. Keeping glucose low for long periods of time is a big challenge which can lead to glucose withdrawal. This study found that prolonged glucose withdrawal led to CHO cells apoptosis and the tolerance time of CHO cells to glucose withdrawal differed with the process conditions and the culture phases. A robust cell culture process allowed CHO cells to tolerate glucose withdrawal longer time, suggesting that the robustness of the process may be judged by the time duration for which CHO cells tolerated glucose withdrawal. Currently, assessment of process robustness is often time-consuming, labor-intensive, and material-intensive by process characterization studies. Therefore a simple and time-saving method is highly needed for the biopharmaceutical industry. The aim of this study was to investigate glucose withdrawal-induced apoptosis and develop a simple and time saving strategy for assessing the robustness of cell culture processes. The strategy we present may be used to expedite the assessment of process robustness to obtain a robust cell culture process for other biologics.
Adaptation of the Tuning Parameter in General Bayesian Inference with Robust Divergence
Authors: Yonekura Shouto , Sugasawa Shonosuke 
Published: July 2022

Abstract
We introduce a novel methodology for robust Bayesian estimation with robust divergence (e.g., density power divergence or γ-divergence), indexed by tuning parameters. It is well known that the posterior density induced by robust divergence gives highly robust estimators against outliers if the tuning parameter is appropriately and carefully chosen. In a Bayesian framework, one way to find the optimal tuning parameter would be using evidence (marginal likelihood). However, we theoretically and numerically illustrate that evidence induced by the density power divergence does not work to select the optimal tuning parameter since robust divergence is not regarded as a statistical model. To overcome the problems, we treat the exponential of robust divergence as an unnormalisable statistical model, and we estimate the tuning parameter via minimizing the Hyvarinen score. We also provide adaptive computational methods based on sequential Monte Carlo (SMC) samplers, which enables us to obtain the optimal tuning parameter and samples from posterior distributions simultaneously. The empirical performance of the proposed method through simulations and an application to real data is also provided.
Partnership Quality and Maternal Depressive Symptoms across the perinatal Period: A prospective Cohort Study
Authors: Schwarze Cornelia E. , Lerche Veronika , Pauen Sabina , Wallwiener Stephanie 
Published: July 2022

Abstract
Purpose. Maternal depression during pregnancy and postpartum is a frequent mental disorder exerting detrimental effects on affected mothers and their infants. A dysfunctional partner relationship during this critical period is recognized as a risk factor for maternal depressiveness. However, the causal relation between partnership quality and perinatal maternal depression has been sparsely studied so far. The current study aims to longitudinally assess relationship quality from pregnancy to postpartum and to test a potential association with maternal perinatal symptoms of depression.Methods. Differing from previous studies, partnership quality and symptoms of depression have been assessed prospectively and longitudinally in a sample of 353 women from the second trimester of pregnancy until six months postpartum. Cross-lagged panel models were applied to investigate the causal relationship between partnership quality and perinatal maternal depressive symptoms.Results. Relationship quality decreased significantly during the transition to parenthood with the steepest decline in tenderness. We found a significant association of relationship quality and maternal symptoms of depression. However, cross-lagged panel models did not confirm the discovered association as causal.Conclusions. The results outline that the transition to parenthood is associated with major changes in the couple’s relationship. However, the cause-and-effect associations may be more complex as previously assumed. Our findings emphasize the importance of providing early prevention and intervention for peripartum women and their partners to improve relationship quality and to prevent adverse outcome for the family.