bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case Series of Three Malaria Patients From Thailand Infected With the Simian Parasite, Plasmodium Cynomolgi
Authors: Sai-ngam Piyaporn , Pidtana Kingkan , Suida Preeyaporn , Poramathikul Kamonporn , Lertsethtakarn Paphavee , Kuntawunginn Worachet , Tadsaichol Sarayut , Arsanok Montri , Sornsakrin Siriporn , Chaisatit Chaiyaporn , Mathavarat Chaiyawat , Thaloengsok Sasikanya , Boonyarangka Parat , Thongpiam Chadin , Demons Samandra , Vesely Brian , Waters Norman C , Saejeng Aungkana , Wojnarski Mariusz , Tabprasit Sutchana , Kwanpichit Chokchai , Greisenbeck John S. , Spring Michele 
Published: January 2022

Abstract
BackgroundWhile human cases of simian malaria, Plasmodium knowlesi, are now regularly recognized in Southeast Asia, infections with other species such as P. cynomolgi are still rare. There has been a handful of clinical cases described, all from Malaysia, and retrospective studies of archived blood samples in Thailand and Cambodia have discovered the presence P. cynomolgi in isolates using polymerase chain reaction (PCR) assays. Case presentationIn Thailand, an ongoing malaria surveillance study enrolled two patients from Yala Province diagnosed with P. vivax by blood smear, but who were subsequently found to be negative by PCR. Expanded PCR testing of these isolates detected mono-infection with P. cynomolgi, the first time this has been reported in Thailand. Upon re-testing of 60 isolates collected from Yala, one other case was identified, a co-infection of P. cynomolgi and P. vivax. The clinical course for all three was relatively mild, with symptoms commonly seen in malaria: fever, chills and headaches. All infections were cured with a course of chloroquine and primaquine.ConclusionIn malaria-endemic areas with macaque populations, cases of simian malaria in humans are being reported at an increasing rate, although still comprise a very small percentage of total cases. P. cynomolgi and P. vivax are challenging to distinguish by blood smear; therefore, PCR can be employed when infections are suspected or as part of systematic malaria surveillance. As Thai MoPH policy schedules regular follow-up visits after each malaria infection, identifying those with P. cynomolgi will allow for monitoring of treatment efficacy, although at this time P. cynomolgi appears to have an uncomplicated clinical course and good response to commonly used antimalarials.
WITHDRAWN: The Effects of Dietary Gracilaria Corticata (Marine Macroalgae, Rhodophyta) Extraction on Growth Performance, Antioxidant Defence, Plasma and Mucosal Immune Components and Immune-related Gene Expressions in Goldfish, Carassius Auratus
Authors: Yumashev Alexei Valerievich , Doewes Rumi Iqbal , Shichiyakh Rustem Adamovich , ahmad Shadia Faris , Suksatan Wanich , Abdelbasset Walid Kamal , Najm Mazin A.A. , Jawad Mohammed Abed , Hoi Huynh Tan , Narimanizad Fariborz 
Published: January 2022

Abstract

The present study examined the effects of the hydro-alcoholic extraction of the red seaweed Gracilaria corticata (GCE), as food additive on growth, antioxidant defence and immunity in the goldfish, Carassius auratus. Four experimental treatments in three replications were established and fed the experimental diets for 60 days. The groups were: a control (fish fed only a basal diet), GCE1: fish supplemented with 0.5 % GCE/kg diet, GCE2: fish supplemented with 1 % GCE/kg diet, GCE3: fish supplemented with 1.5 % GCE/kg diet. After feeding period, the antioxidant [superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT)] and immune responses were evaluated. Dietary GCE had no effect on growth performance (P>0.01). The plasma immune responses including alternative complement (ACH50) and lysozyme activities elevated in fish supplemented with 1 % and 1.5 % GCE compared to those fed 0.5 % GCE and basal diet (P<0.01). The plasma and mucosal immunoglobulin (Ig) significantly elevated in all GCE supplemented fish (P<0.01). The plasma peroxidase activity significantly increased only in the fish receiving GCE at dietary level of 1.5 % GCE compared to control and those supplemented with 0.5 % and 1 % GCE (P<0.01). The mucosal lysozyme, protease activity and alkaline phosphatase significantly increased in fish supplemented with 1 % GCE compared to other experimental diets (P<0.01). The activity of antioxidant enzymes (SOD, CAT, GPx) showed significant increases in fish supplemented with 1 % and 1.5 % GCE (P<0.01). Furthermore, the expression of the immune-related genes, complement and lysozyme significantly elevated in the treatments of 1 % and 1.5 % GCE compared to other experimental diets (P<0.01). The results of this study revealed that the use of GCE as a food additive in the diet of goldfish diet can improve the fish immunity without negative impacts on growth.
Effect of Mucuna Pruriens Seeds Supplementation on Physiological, Hemato-Biochemical, Antioxidant, Thyroid, and Progesterone Profile of Beetal Does in Relation to Seasonal Stress
Authors: Jadhav Sameer Niwas , Nayyar Shashi , Honparkhe Mrigank , Singla Mandeep 
Published: January 2022

Abstract
A study was conducted to analyze the effect of raw Mucuna pruriens seed supplementation on the physiological adaptability of Beetal goat does to seasonal stress. Supplementation with raw Mucuna pruriens seed powder was done at lower level of 100 mg/kg bodyweight for 30 days to beetal does during winter and summer season. Blood samples were collected on 0, 15,30 and 45th day. Temperature humidity index was found higher (83.08±0.35) during the summer season, while relative humidity remained high (63.77±2.32) during the winter season. Data analysis by Analysis of covariance (ANCOVA) revealed that compared to control, Mucuna pruriens supplementation in goats resulted in a significant decrease in heart rate during the summer season, increase in respiration rate in the winter season, decrease in overall glucose, cholesterol, creatinine, blood urea nitrogen, Erythrocytic lipid peroxidation (MDA) level and increase in packed cell volume, hemoglobin, total erythrocyte and leukocyte count, total protein, albumin, alkaline phosphatase, immunoglobulin, reduced glutathione and total antioxidant capacity levels indicating positive anabolic effect. A significant increase in plasma progesterone in Mucuna pruriens supplemented does compare to control was observed during winter season. It may be concluded that supplementation of raw Mucuna pruriens at lower levels were found to be efficient nutraceutical supplement option for does feeding and has a positive effect on amelioration of seasonal stress & improvement in health and sustainability of Beetal does.
Risk Factors for Transaminase Elevation in Children with Epstein-Barr Virus Infectious Mononucleosis
Authors: Chen Qian , Zhai Yiman , Liu Wenjun 
Published: January 2022

Abstract
Objective: To study the risk factors of Epstein-Barr virus (EBV) infectious mononucleosis (EBV-IM) in children with elevated transaminase. Methods: A retrospective analysis of the clinical data of 533 children with EBV-IM who were hospitalized and diagnosed in the Affiliated Hospital of Southwest Medical University from January 1, 2018, to May 31, 2021. The alanine aminotransferase (ALT)> 40U/L or aspartate aminotransferase (AST)>40U/L was defined as elevated transaminase and divided into normal group and abnormal group based on that. Monofactor analysis and logistic regression analysis were used to explore the risk factors of EBV-IM with the elevation of transaminase, and the receiver operating characteristic (ROC) curve was drawn to evaluate the predictive ability of each indicator. Results: Of the 533 cases, 428 cases (80.3%) were in the transaminase elevation group，there were 277 cases(52.0%) of ALT elevation and 414 cases(77.7%) of AST elevation. The multifactor Logistic regression showed that the fever, age, γ-glutamyl transferase(GGT), atypical lymphocyte(ALYM), and albumin(ALB) were associated with elevated ALT (OR=0.351, 1.140, 1.053, 1.032, 0.912, All P<0.05) and age, GGT, ALYM, ALB predict the area under the ROC curve(AUC) of EBV-IM with ALT elevation were 0.665, 0.890, 0.579, 0.669,and GGT, mean platelet volume to platelet count(MPV/PLT%) were correlated with increased AST(OR=1.029, 1.220, all P<0.05 ) the AUC predicted GGT and MPV/PLT(%) for EBV-IM and AST increase were 0.715 and 0.623.Conclusion: With EBV-IM in children, Fever, age, GGT, ALYM, and ALB are risk factors for ALT elevation, and GGT, MPV/PLT(%) are risk factors for AST elevation.
Exploring Multi-Disciplinary Cancer Care for Immunotherapy-Related Dermatological Symptoms
Authors: Kruger Dikla , Samuels Noah , Lacey Judith , Keshet Yael , Gressel Orit , Dodiuk-Gad Roni , Shulman Katerina , Tapiro Yehudit , Golan Miri , Ben-Arye Eran 
Published: January 2022

Abstract
Context and objectives: The present study examined the perspectives of healthcare providers (HCPs) in designing a multi-disciplinary model of supportive cancer care for the relief of dermatology-related symptoms caused by immunotherapy. Methods: This qualitative study identified patients undergoing oncology therapy with MoAB, anti-HER2 and anti-PD-L1 monoclonal antibodies among a cohort of patients referred to an integrative oncology (IO) consultation for symptom relief and improved quality of life (QoL). Case-studies with significant dermatology-related concerns were selected and presented to a panel of 6 HCPs trained in medical oncology, oncology nursing, family medicine, supportive cancer care and IO. HCP narratives were qualitatively analyzed and assessed using ATLAS.Ti software for systematic coding. Results: Of 924 patients referred to the IO consultation, 208 were treated with immunotherapy, from which 50 were selected for further evaluation. Of these, and 7 cases were presented to the HCP team which then identified treatment gaps requiring a multi-disciplinary approach. Qualitative analysis identified 3 major themes: a biophysical perspective; a psycho-social-spiritual perspective; and the implementation of integrated care. Discussion: There is a need for a multi-disciplinary approach when treating patients suffering from immunotherpay-related skin toxicities. HCP-reported themes highlight the need to identify patients where such an approach is warranted; conditions where a psycho-social-spiritual perspective should be considered in addition to the bio-physical approach; and considerations of who should be appointed as the patient’s case manager.
Analysis of Some Phytochemicals as Potential Anticancer Agents Targeting the Receptor Protein Associated With Cancer
Authors: Sen Rishabh , Ghosh Subarna , Datta Anindita 
Published: January 2022

Abstract
Cancerous growth is characterized as the uncontrolled multiplication and spread of the body's particular cells causing infection and addresses one of the greatest medical care issues for humanity and requests a proactive procedure for fix. Sensitization of cancer cells to conventional drugs using multi-target agents that block survival and oncogenic pathways, alone or in combination, is an emerging strategy to overcome drug resistance. Plants are natural reservoirs of novel compounds and provide a promising therapeutic approach for treatment of cancer. Phytochemicals are viewed as appropriate possibility for anticancer medication advancements due to their pleiotropic activities on track occasions with numerous habits. These phytochemicals frequently act by means of regulating molecular pathways which are embroiled in development and dissemination of cancer. The particular strategies in cancer prevention involves tracking of anti-carcinogenic agents, carcinogen inactivation, repressing proliferation, induction of cell cycle capture and apoptosis; and regulating the immune system. Presently, research is in progress for search of novel phytochemicals having anti cancerous properties with minimum or zero side effects. Numerous phytochemicals and their determined analogs have been recognized as possible contender for anticancer treatment and the present review article sheds light on few of these phytocompounds.
Comprehensive Evaluation of Chinese Peanut Mini-Mini Core Collection and QTL Mapping for Aflatoxin Resistance
Authors: Ding Yingbin , Qiu Xike , Luo Huaiyong , Huang Li , Guo Jianbin , Yu Bolun , Sudini Hari , Pandey Manish , Kang Yanping , Liu Nian , Zhou Xiaojing , Chen Weigang , Chen Yuning , Wang Xin , Huai Dongxin , Yan Liying , Lei Yong , Jiang Huifang , Varshney Rajeev , Liu Kede , Liao Boshou 
Published: January 2022

Abstract
Background
Aflatoxin contamination caused by Aspergillus fungi has been a serious factor affecting food safety of peanut (Arachis hypogaea L.) because aflatoxins are highly harmful for human and animal health. As three mechanisms of resistance to aflatoxin in peanut including shell infection resistance, seed infection resistance and aflatoxin production resistance exist among naturally evolved germplasm stocks, it is highly crucial to pyramid these three resistances for promoting peanut industry development and protecting consumers’ health. However, less research effort has been made yet to investigate the differentiation and genetic relationship among the three resistances in diversified peanut germplasm collections.
Results
In this study, the Chinese peanut mini-mini core collection selected from a large basic collection was systematically evaluated for the three resistances against A. flavus for the first time. The research revealed a wide variation among the diversified peanut accessions for all the three resistances. Totally, 14 resistant accessions were identified, including three with shell infection resistance, seven with seed infection resistance and five with aflatoxin production resistance. A special accession, Zh.h1312, was identified with both seed infection and aflatoxin production resistance. Among the five botanic types of A. hypogaea, the var. vulgaris (Spanish type) belonging to subspecies fastigiata is the only one which possessed all the three resistances. There was no close correlation between shell infection resistance and other two resistances, while there was a significant positive correlation between seed infection and toxin production resistance. All the three resistances had a significant negative correlation with pod or seed size. A total of 16 SNPs/InDels associated with the three resistances were identified through genome-wide association study (GWAS). Through comparative analysis, Zh.h1312 with seed infection resistance and aflatoxin production resistance was also revealed to possess all the resistance alleles of associated loci for seed infection index and aflatoxin content.
Conclusions
This study provided the first comprehensive understanding of differentiation of aflatoxin resistance in diversified peanut germplasm collection, and would further contribute to the genetic enhancement for resistance to aflatoxin contamination.
Ficolin-2 Plasma Levels Assesses Liver Fibrosis in Non-Alcoholic Fatty Liver Disease
Authors: Giraudi Pablo José , Salvoza Noel , Bonazza Deborah , Saitta Carlo , Lombardo Daniele , Casagranda Biagio , de Manzini Nicolò , Pollicino Teresa , Raimondo Giovanni , Tiribelli Claudio , Palmisano Silvia , Rosso Natalia 
Published: January 2022

Abstract
Background & Aims: In the next 20 years, non-alcoholic fatty liver disease (NAFLD) is expected to become the leading cause of liver transplantation. Fibrosis is the most important prognostic factor for liver-related outcomes and mortality, but the need for liver biopsy limits diagnosis. We assessed the performance of plasma ficolin-2 (FCN-2) as a biomarker of fibrosis. FCN-2 candidate was selected by an in silico discovery strategy. Methods: We enrolled 235 morbidly obese (MO) subjects with biopsy-proven NAFLD stratified by fibrosis stage (F0, n=44; F1, n=134; F2, n=46; F3/F4, n=11) and 40 cirrhotic patients as positive controls. The cohort was subdivided into discovery (n=76) and validation groups (n=159). The plasma level of FCN-2 and other biomarkers was determined by enzyme-linked immunoabsorbent assay. Results: Plasma level of FCN-2 correlated inversely with the stage of liver fibrosis (ρ = -0.49, p<0.0001), independently of steatosis (p=0.90), inflammation (p=0.57), and ballooning (p=0.59). In the global cohort, FCN-2 level decreased significantly in a stepwise fashion from F0/F1 (median 4753 ng/mL) to F2-F3-F4 (2760 ng/mL) and in cirrhotic subjects (1418 ng/mL). The diagnostic performance of FCN-2 in detecting F≥2 fibrosis was higher than that of other fibrosis indexes (APRI, FIB-4, NFS) (AUROC 0.82, 0.68, 0.67, and 0.68, respectively), sh o wing a substantially improved accuracy when combined with APRI score and HDL plasma values in a diagnostic index (FCNscore, AUROC 0.85). Conclusion: FCN-2 plasma level can accurately discriminate liver fibrosis status (minimal vs . moderate/advanced). Its inclusion in the diagnostic workup significantly improves the fibrosis diagnostic algorithms.
An Environmental Scan of Ontario’s Mental Health and Addiction Services Exclusive to LGBTQ2S+ Youth During COVID-19
Authors: Chaiton Michael , Billington Rebecca , Copeland Lani , Grey Luc , Abramovich Alex 
Published: January 2022

Abstract
Background: LGBTQ2S youth experience mental health disparities and higher rates of substance use when compared to their cisgender and heterosexual peers yet also experience more barriers to access to services. The purpose of this environmental scan is to determine the types of mental health and substance use programs and services exclusive to LGBTQ2S youth in Ontario and how these services have changed during the pandemic. Methods: This environmental scan was conducted to identify existing programs and services in Ontario that offered exclusive mental health and addiction services to LGBTQ2S+ individuals aged 16-29, either by offering services to all or subgroups within the population. Results: In total, 113 organizations and 240 programs and services were identified as providing mental health and substance use services exclusively to LGBTQ2S+ youth. Four main themes were identified from the scan, including the distribution of services, types of services, methods of service delivery and program criteria. Adaptations for the COVID pandemic included cancelling in-person services, increasing online and telephone services, and expansion to province-wide from local availability. Conclusions: The findings from this scan highlight the importance of offering services that provide culturally inclusive care for LGBTQ2S+ youth, and these results can also be used by policy makers to inform policies. In particular, there was a lack of culturally-relevant clinical services for youth requiring greater intensity of treatment.
A Spectrum of Non-Traumatic Urological Emergencies in a Single Center in Tunisia: Ten Years Experience.
Authors: Moez Rahoui , Rami Boulma , Hassen Khouni 
Published: January 2022

Abstract
Background:Emergency department consultations are the most important in any hospital. Although nontraumatic urological emergencies are a common reason for hospitalization in a urological environment. The aim of our study was to present the epidemiological and therapeutic aspects of non-traumatic urological emergencies in our hospital for 10 years.Methods:This was a retrospective study of records of patients who presented with non-traumatic Urological emergencies to our institution, over a 10-year period (from January 2010 – December 2019). This study was done in the Department of Urology in the hospital of the Internal Security Forces La Marsa. The data extracted included; the demographic information, diagnosis, and the treatment offered. The data obtained were analyzed using SPSS version 21. Data were displayed using mean +/− standard deviation and percentages.Results:We registered 531 patients. The average age of our patients was 48.2 years (18 to 94 years). The sex ratio (M / F) was 2/1. These patients were over or equal to 50 years in 45.7% of cases. The most frequent conditions were acute urine retention (43%) and urogenital infections which together accounted for 18.4% of cases. The two other diagnoses the most retained were: obstructive lithiasis pyelonephritis in 11.9% (64 patients) including 12 cases of emphysematous pyelonephritis, hyperalgesia renal colic in 5.9% of cases. Testicular torsion constituted the third commonest urological emergency seen, accounting for 3,36% (n = 18) of the cases. Gangrene of the external genitalia accounted for 1.9% of urologic emergencies, and priapism 1.3%. 138 patients were hospitalized in the urology unit for treatmentConclusion:The most common nontraumatic urological emergency was urine retention in the elderly. To our knowledge, this study is the first to detail the activity of non-traumatic urological emergencies in Tunisia, and it will certainly be the starting point for the establishment of a structure dedicated entirely to urological activity.
Throat Carriage rate, Associated Factors, and Antimicrobial Susceptibility Pattern of Group A Streptococcus Among Healthy School Children in Jigjiga City, Eastern Ethiopia
Authors: Barsenga Shamil , Miteku Habtamu , Tesfa Tewodros , Shume Tadesse 
Published: January 2022

Abstract
Background: Group A Streptococcus has been recognized as an important human pathogen and it remains among the top ten causes of mortality from an infectious disease. Group A Streptococcus throat carriage plays an important role in the development of infection and transmission to contacts. In Ethiopia, there is little information about screening of children for Group A Streptococcus carriage.Objective: This study was aimed to assess the magnitude of throat carriage, associated factors, and antimicrobial susceptibility pattern of Group A Streptococus among healthy school children in Jigjiga city, Eastern Ethiopia from 12 April to 27 May 2021.Method: A cross-sectional study was conducted among 462 healthy school children in ages ranging from 7 to 14 years. The throat sample was collected using a sterile cotton swab. Identification of Group A Streptococcus was done by colony characterstics, gram staining, catalase negativity, bactricin sensitivity and Pyrrolidonyl arylamidase tests. Antibiotic susceptibility test was done on Muller-Hinton agar containing 5% sheep blood by modiﬁed Kirby-Bauer disc diffusion method. Data on socio-demographic and related characteristics were gathered through interviews using a questionnaire. The data were double-checked, coded, cleaned, and entered onto EpiData Version 3.1 then exported to SPSS version 26.0 for analysis. Bivariate and multivariable logistic regression was used to determine the relationship between outcome and predictor variables. A p-value < 0.05 was taken as statistically significant.Results: The overall prevalence of Group A Streptococcus carriage was 10.6% (95%CI; 8.1% - 13.7%). Children who live with a family member with a sore throat (AOR=2.51; 95%CI 1.09–5.73), children who live with a large family (AOR=4.64; 95% CI 1.53–14.1), and children who live with non-immediate families (AOR= 3.65; 95% CI 1.39 – 9.61), showed significant association with Group A Streptococcus carriage. Tetracycline resistance was shown to be high, while antimicrobial drugs such as penicillin, amoxicillin, ceftriaxone, erythromycin, azithromycin, chloramphenicol, and vancomycin were found to be effective. Multi-drug resistance was found in 4.1 % of the isolates. Conclusion: The present study showed a significant throat carriage of Group A Streptococci/S. pyogenes in Jigjiga city school children. Family member with a sore throat, having a large family, and living with non-immediate families have all been identified as independent predictors of carriage prevalence. It is recommended that regular screening and surveillance should be conducted in schools.