bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete Genome Sequence and Phylogenetic Analysis of a Goose Astrovirus Isolate in China
Authors: Tan ZHENG , Zhai Hui , Sun Ruqi , Xie Ruyu , Sun Zhe , Zhang Lilin , Li Zexing , huang jinhai 
Published: January 2022

Abstract
Astroviruses are considered the cause of gastroenteritis in humans and animals. Studies in recent years show avian astroviruses are also associated with duckling hepatitis, gosling gout, and chicken nephritis. In this study, a GAstV strain, designated as JS2019/China, was detected in dead goslings from a commercial goose farm in Jiangsu province of China. Viral strain was proliferated in goose embryos and sequence analysis showed the isolated strain had a classical structure arrangement and a series of conserved regions compared with other GAstVs. Sequence comparison and phylogenetic analysis of whole genome and ORF2 revealed that JS2019/China belongs to the GAstV group-1, which consists of most of the GAstV strains. Amino acid analysis indicated that some mutants might have an impact on viral protease capacity, such as V505I and K736E of ORF1a and T107I, F342S, and S606P of ORF2. Taken together, a novel GAstV strain was isolated and genomic analysis and protein polymorphism analysis indicated that some amino acid mutants might affect the viral virulence.
Changes in Tillering, Nutritional Status, and Biomass Yield of Panicum Maximum Used for Cadmium Phytoextraction
Authors: Rabêlo Flávio Henrique Silveira , Santos Felipe dos , Lavres José , Alleoni Luís 
Published: January 2022

Abstract
Although several grasses have been evaluated for cadmium (Cd) phytoextraction, there are no studies assessing how Cd is accumulated and distributed in the tissues of Panicum maximum grown in mildly polluted soils. The evaluation of tillering, nutritional status and biomass yield of this grass, mainly along successive shoot regrowths, is not well studied so far. Thus, P. maximum Jacq. cv. Massai was grown for two periods in an Oxisol presenting bioavailable Cd concentrations varying from 0.04 (control) to 10.91 mg kg−1 soil. Biomass yield of leaves and stems´ growth have decreased under the highest Cd exposure, but it did not occur in the regrowth period, indicating that Cd-induced toxicity is stronger in the early stages of development of P. maximum. The tillering was not compromised even the basal node presenting Cd concentrations higher than 100 mg kg−1 DW. We identified a restriction on Cd transport upwards from basal node, which was the main local of Cd accumulation. Apparently, P, K, Mg, S and Cu are involved in processes that restrict Cd translocation and confer high tolerance to Cd in P. maximum. The Cd-induced nutritional disorders did not negatively correlate with factors used to calculate phytoextraction efficiency. However, the nutritional adjustments of P. maximum to cope with Cd stress restricted the upward Cd transport, which decreased the phytoextraction efficiency from the available Cd concentration of 5.93 mg kg−1 soil.
Understanding Mentoring Style of Mentors and Science Identity Formation in Historically Excluded Undergraduate Students in Natural History Collections
Authors: Pirlo Jeanette 
Published: January 2022

Abstract

Background: Science identity formation is integral to the transformation from a student to a member of the STEM workforce. However, the formation of a student’s science identity is affected by various factors such as support from mentors and community members, the student’s perception of their peers’ acceptance of them, as well as the student’s own perception of their role in research. The aim of this study is to investigate mentors’ understanding of science identity formation, their mentoring style, and if it is effective when working with students from historically excluded populations. A phenomenological approach was used to conduct this study. Structured interviews were conducted with natural history collections faculty members of various backgrounds and career levels. Results: Three themes emerged that contribute to science identity formation through mentoring: 1. Factors for science identity formation; 2. Mentorship among different career stages, and 3. Defining own mentoring style. Predominantly, participants realized that their own experience as a mentee shapes how they approach mentoring.Conclusions: Although this study describes the mentee’s science identity formation from the mentor’s point of view, the study’s participants realized the importance they play in a student’s science identity formation, and that mindful mentoring is necessary for success when engaging historically excluded groups in the STEM workforce.
Effects of ultrasonic surface rolling process on tribological behavior of 4Cr13 stainless steel
Authors: Luo Xiaoshuang , Zhan Shengpeng , Jia Dan , Tu Jiesong , Li Yinhua , Yang Tian , Duan Haitao 
Published: January 2022

Abstract
Ultrasonic surface rolling (USR) process is a novel surface strengthening technique based on the tool head's high-frequency impact on the workpiece. USR can cause severe plastic deformation on the superficial surface of metal material, and greatly improving the mechanical properties of the material. This paper elucidates the effects of USR passes on the surface roughness, sample height, microstructure, microhardness, residual stress, and tribological properties of 4Cr13 stainless steel. The results revealed that multiple USR treatments refined the near-surface layer grain of the sample. Compared with untreated sample, USR treatments significantly improved the surface roughness and microhardness of the samples. Obvious compressive residual stress and plastic deformed with a maximum value of about -723 MPa and a depth of about 229 μm were also introduced into the sample surface. Under a dry friction environment, the samples that underwent the USR treatments exhibited significantly enhanced wear resistance, and six rolling passes were found to be the most suitable treatment.
Prospective Study on the Performance of 18F-DOPA Positron Emission Tomography/Computed Tomography in Patients with Medullary Thyroid Carcinoma
Authors: Califano Inés , Pitoia Fabian , Chirico Roxana , de Salazar Alejandra , Bastianello Maria 
Published: January 2022

Abstract
Purpose

18F-DOPA Positron Emission Tomography/Computed Tomography (18F-DOPA PET/CT) is a sensitive functional imaging method (65-75%) for detecting disease localization in medullary thyroid cancer (MTC). We aimed: i) to assess the clinical usefulness of 18F-DOPA PET/CT in patients with MTC and elevated calcitonin (Ctn) and CEA levels and, ii) to evaluate changes in disease management secondary to the findings encountered with this methodology.
Methods

thirty-six patients with MTC and Ctn levels ≥150 pg/ml were prospectively included. Neck ultrasound, chest contrast-enhanced CT, liver magnetic resonance imaging/ abdominal 3-phase contrast-enhanced CT and bone scintigraphy were carried out up to 6 months before the 18F DOPA PET/CT.
Results

77.7% were female and 27% had hereditary MTC. Median Ctn level was 1450 pg/ml [150-56620], median CEA level 413 ng/ml [2.9-7436]. Median Ctn DT was 37.5 months [5.7-240]; median CEA DT was 31.8 [4.9-180]. 18F-DOPA PET/CT was positive in 33 patients (91.6%); in 18 (56%) uptake was observed in lymph nodes in the neck or mediastinum, in 7 cases (22%) distant metastases were diagnosed, and in 8 additional patients (24%) both locoregional and distant sites of disease were found. Ctn and CEA levels were higher in patients with ≥ 3 foci of distant metastases. In 14 patients (38.8%), findings on 18F-DOPA PET/CT led to changes in management; surgery for locoregional lymph nodes was the most frequent procedure in 8 patients (22%).
Conclusion

18F-DOPA PET/CT was useful for the detection of recurrent disease in MTC and provided helpful information for patient management.
High expression of TMEM33 predicts poor prognosis and promotes cell proliferation in cervical cancer
Authors: Chen Hanxiang , Li Yongqing , Zhang Shaoming , Wang Yunshan , Wang Lili , Ma Wanshan 
Published: January 2022

Abstract
Background

As one of the most common cancer among women worldwide, the prognosis of patients with advanced cervical cancer remains unsatisfactory. A study indicated that transmembrane protein 33 (TMEM33) was implicated in tumor recurrence, while its role in cervical cancer has not been elucidated.
Methods

TMEM33 expression in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) was primarily screened in The Cancer Genome Altas (TCGA), and further validated in Gene Expression Omnibus (GEO) database. The Kaplan–Meier plotter analysis and Cox regression were constructed to evaluate the prognostic value of TMEM33 in CESC. Functional enrichment analysis was performed with GO, KEGG and GSEA tools. Protein-protein interaction analysis and correlated gene networks were conducted using STING and GEPIA2 websites, respectively. The expression of TMEM33 in cervical cancer cells were examined by immunoblotting and RT-qPCR. Finally, CCK-8 assay and colony formation assay were performed to investigate the role of TMEM33 in cervical cancer cell proliferation.
Results

TMEM33 expression was significantly elevated in CESC compared with normal tissues. High expression of TMEM33 was associated with poor prognostic clinical characteristics in CESC patients. KM-plotter analysis revealed that patients with increased TMEM33 had shorter overall survival (OS), progress free interval (PFI), and disease specific survival (DSS). Moreover, Multivariate Cox analysis further confirmed that high TMEM33 expression was an independent risk factor for OS in patients with CESC. TMEM33 was associated with immune cell infiltration, and its expression was correlated with tumorigenesis-related genes RNF4, OCIAD1, TMED5, DHX15, MED28 and LETM1. More importantly, knockdown of TMEM33 in cervical cancer cells decreased the expression of those genes and inhibited cell proliferation.
Conclusions

Increased TMEM33 in cervical cancer can serve as an independent prognostic marker and might play a role in tumorigenesis by promoting cell proliferation.
Prevalence of Posttraumatic Stress Disorder and Depression Among Internally Displaced Persons In Mogadishu
Authors: Ali Mustafa , Mutavi Teresia , Mathai Muthoni , Mburu John 
Published: January 2022

Abstract
Background

Nearly three decades of conflict and frequent droughts and environmental hardships, have left 2.6 million of Somalis in displacement camps. Even though psychological impact of war and natural disasters are well documented, little is known about the unseen scars of psychological trauma in Internally displaced persons in Somalia. The purpose of the study was to determine the prevalence of post-traumatic stress disorder (PTSD) and depression amongst internally displaced persons (IDPs), and examine association between displacement and these psychiatric conditions.
Methodology

A cross-sectional quantitative study was conducted among 406 IDPs in Mogadishu. Harvard Trauma Questionnaire was used to determine levels of trauma exposure and PTSD, and Hopkins Symptom Checklist-25 was used to estimate prevalence of depression. Multivariate and bivariate analysis was conducted to analyze the association of demographic and displacement variables on the outcomes of PTSD and depression.
Results

More than half (59%) of participants met the symptom criteria of depression, and nearly one third (32%) of respondents met the symptom criteria for PTSD. The most prevalent traumatic event was lack of food or water (80.2%). Important predictive factors in development of psychiatric morbidity were unemployment, cumulative traumatic exposure, frequency and duration of displacement.
Conclusion

The study revealed high levels of Depressive disorder and Post-Traumatic Stress Disorder among internally displaced persons in Mogadishu. Furthermore, this study provided evidence to IDPs’ susceptibility to trauma exposure and lack of essential services and goods. Study also highlighted the importance of provision of Mental Health and Psychosocial Support (MHPSS) service in IDP camps.
Ferroptosis-Related lncRNA Signature Predicts Prognosis and Immunotherapy Efficacy in Cutaneous Melanoma
Authors: Xu Yujian , Chen Youbai , Niu Zehao , Yang Zheng , Xing Jiahua , Yin Xiangye , Guo Lingli , Zhang Qixu , Yang Yi , Han Yan 
Published: January 2022

Abstract
Ferroptosis-related lncRNAs are promising biomarkers for predicting the prognosis of many cancers. However, a ferroptosis-related signature to predict the prognosis of cutaneous melanoma (CM) has not been identified. The purpose of our study was to construct a ferroptosis-related lncRNA signature to predict prognosis and immunotherapy efficacy in CM. Ferroptosis-related differentially expressed genes (FDEGs) and lncRNAs (FDELs) were identified using TCGA, GTEx, and FerrDb datasets. We performed Cox and LASSO regressions to identify key FDELs, and constructed a risk score to stratify patients into high- and low-risk groups. A nomogram was developed for clinical use. We performed gene set enrichment analyses (GSEA) to identify significantly enriched pathways. Differences in the tumor microenvironment (TME) between the 2 groups were assessed using 7 algorithms. To predict the efficacy of immune checkpoint inhibitors (ICI), we analyzed the association between PD1 and CTLA4 expression and the risk score. Finally, differences in Tumor Mutational Burden (TMB) and molecular drugs Sensitivity between the 2 groups were performed. Here, we identified 5 lncRNAs (AATBC, AC145423.2, LINC01871, AC125807.2, and AC245041.1) to construct the risk score. The AUC of the lncRNA signature was 0.743 in the training cohort and was validated in the testing and entire cohorts. Kaplan-Meier analyses revealed that the high-risk group had poorer prognosis. Multivariate Cox regression showed that the lncRNA signature was an independent predictor of OS with higher accuracy than traditional clinicopathological features. The 1-, 3-, and 5-year survival probabilities for CM patients were 92.7%, 57.2%, and 40.2% with an AUC of 0.804, indicating a good accuracy and reliability of the nomogram. GSEA showed that the high-risk group had lower ferroptosis and immune response. TME analyses confirmed that the high-risk group had lower immune cell infiltration (e.g., CD8+ T cells, CD4+ memory-activated T cells, and M1 macrophages) and lower immune functions (e.g., immune checkpoint activation). Low-risk patients whose disease expressed PD1 or CTLA4 were likely to respond better to ICIs. The analysis demonstrated that the TMB had significantly difference between low- and high- risk groups. Chemotherapy drugs, such as sorafenib, Imatinib, ABT.888 (Veliparib), Docetaxel, and Paclitaxel showed Significant differences in the estimated IC50 between the two risk groups. Overall, our novel ferroptosis-related lncRNA signature was able to accurately predict the prognosis and ICI outcomes of CM patients. These ferroptosis-related lncRNAs might be potential biomarkers and therapeutic targets for CM.
Aluminium leaching in fluid warming devices – A Review
Authors: Bayoro Danielle K. , Hoolihan Daniel , Pedro Michael J , Rose Edward A. , Waldmann Andreas D. 
Published: January 2022

Abstract
Current guidelines recommend the use of an intravenous fluid warmer to prevent perioperative hypothermia. Among the various methods of warming intravenous fluids, contact warmers are among the most effective and accurate, particularly in clinical conditions requiring rapid infusions of refrigerated blood or fluids. Contact warmers put the infusate in direct contact with a heating block. Some fluid warmers use heating blocks manufactured from aluminium. Several recent publications, however, have shown that uncoated aluminium blocks can leach potentially toxic amounts of aluminium into the body.
In this review we performed a systematic literature review on aluminium leaching with contact fluid warmers and describe what manufacturer and competent authorities did in the past years to ensure patient safety. The search resulted in five articles describing the aluminium leaching. Four different devices (Level 1 Fluid Warmer from Smiths Medical, ThermaCor from Smisson-Cartledge Biomedical, Recirculator 8.0 from Eight Medical International BV, enFlow from Vyaire) were shown to leach high levels of aluminium when heating certain intravenous fluids. One manufacturer (Vyaire) voluntarily removed their product from the market, while three manufacturers (Eight Medical International BV, Smisson-Cartledge Biomedical, and Smiths Medical) revised the instructions for use for the affected devices. The enFlow fluid warmer was subsequently redesigned with a parylene coating over the heating block. The scientific literature shows that by using a thin parylene layer on the heating block, the leaching of aluminium can be nearly eliminated without affecting the heating performance of the device.
S ilencing of SCD and SREBP1 Genes by Short Hairpin RNAs in Chicken Hepatic Cells in Vitro
Authors: Sagar N Govardhana , Prasad A Rajendra , Kumar Pushpendra , Bhushan Bharat , Vishnu P Guru , Bhattacharya Tarun K 
Published: January 2022

Abstract
RNA interference by short hairpin RNAs (shRNAs) is a widely used post transcriptional silencing mechanism for suppressing expression of the target gene. In the current study, five shRNA molecules each against SCD and SREBP1 genes involved in denovo lipid biosynthesis were designed upon considering parameters such as secondary structures of shRNAs, mRNA target regions, GC content and thermodynamic properties (ΔG overall, ΔG duplex and ΔG break-target), synthesized and cloned in pENTR/U6 entry vector to knockdown the expression of SCD and SREBP1 genes. After transfection of these shRNA constructs into the chicken embryonic hepatocytes, expressions of the target genes were monitored by real time PCR. Significant reduction (P<0.05) in the expression of SCD and SREBP1 genes was observed in hepatocytes. The shRNAs against SCD gene showed the knock down efficiency ranged from 20.4% (shRNA5) to 74.2% (shRNA2). In case of SREBP1 gene, the shRNAs showed knock-down efficiency ranging from 26.8% (shRNA4) to 95.85% (shRNA1). The shRNAs against both the genes introduced in chicken hepatocyte cells did not show any significant impact on expression of immune response genes (IFNA and IFNB) in those cells. These results clearly demonstrated the successful down regulation of the expression of SCD and SREBP1 genes by the shRNA molecules against both the target genes under in vitro condition. It is concluded that the shRNA molecules against SCD and SREBP1 genes showed great potential to silence the expression of these genes under in vitro chicken embryonic hepatocyte cells.
Attribute Reduction Algorithm of Neighborhood Rough Set Based on Supervised Granulation and Its Application
Authors: zou li , Ren Siyuan , Sun Yibo , Yang Xinhua 
Published: January 2022

Abstract
In neighborhood rough set theory, attribute reduction based on measure of information has important application significance. The influence of different decision classes was not considered for calculation of traditional conditional neighborhood entropy, and the improvement of algorithm based on conditional neighborhood entropy mainly includes of introducing multi granularity and different levels, while the mutual influence between samples with different labels is less considered. To solve this problem, this paper uses the supervised strategy to improve the conditional neighborhood entropy of three-layer granulation. By using two different neighborhood radii to adjust the mutual influence degree of different label samples, and by considering the mutual influence between conditional attributes through the feature complementary relationship, a neighborhood rough set attribute reduction algorithm based on supervised granulation is proposed. Experiment results on UCI data sets show that the proposed algorithm is superior to the traditional conditional neighborhood entropy algorithm in both aspects of reduction rate and reduction accuracy. Finally, the proposed algorithm is applied to the evaluation of fatigue life influencing factors of titanium alloy welded joints. The results of coupling relationship analysis show that the effect of joint type should be most seriously considered in the calculation of stress concentration factor. The results of influencing factors analysis show that the stress range has the highest weight among all the fatigue life influencing factors of titanium alloy welded joint.
Framework for Furniture and Wood Processing Industry Polytechnic in Era Disruption of Covid-19
Authors: Kristianto Fesa Putra , Fariz Nuthqy , Saputra Deni , Athirah Salwa Islami 
Published: January 2022

In the era of the Covid-19 pandemic, all activities are forced to adapt to the Disruption Era, one of which is the education sector. The era of Disruption Covid-19 changed the learning system from face to face to online and did not change the quality of the learning process. Therefore, the Furniture and Wood Processing Industry Polytechnic must create a new framework in order to survive. This study aims to create a new framework that has the perspectives of various stakeholders such as government, industry, academic institutions, certification institutions, students and technological developments. The making of this framework is based on PEST analysis and a drives competitive for universities according to (Panday, 2014). The result of the framework for the Wood Furniture and Processing Industry Polytechnic are implementation health protocol for Covid-19, improvement of facilities dan infrastructure, creating income from teaching factory, SDM adaptation for improvement facilities and infrastructure, and quality assurance of graduates. The next step is to carry out monitoring and evaluation (MONEV) so that the implementation of framework runs according to the references and targets.
Organizational Change in Public Service
Authors: Ekowati Dian 
Published: January 2022

This study aims to investigate institutional logic underlying the initiation of the change management process in public sector organizations. Organizational Institutionalism is used to frame the analysis. The study itself took place in three different public organizations in the Province of East Java, including a one-stop service for licensing as well as that of vehicle registration, taxing, and insurance and also from the local health authority. The qualitative method was employed to analyze information gathered through semi-structured interviews with 35 respondents. Data were analyzed by using an approach informed by grounded theory. The study unveiled various institutional logics underlying the adoption of changes and innovation in organizations. It ranged from the most common reason for improving performance gaps up to the need to clean the organization’s name and build a better image. The study contributes to the idea that an organization’s previous experiences, as well as perceived institutional character, affect the need to conduct changes.
Forecasting of Tourism Companies Before and During Covid-19
Authors: Sarkar Asima 
Published: January 2022

For about last two years, the whole world is suffering from a novel disease i.e. Covid-19. When it was first diagnosed in China, even the giant health agencies could not predict the severity and spread of this disease. Slowly, when this novel corona virus had an outbreak the countries stopped all kinds of communication be it interstate or intercountry and so the tourism companies started facing huge loss due to lockdown in every single country. In this paper, the stock prices of the multinational tourism companies that operate in India, have been forecasted and using an online learning algorithm known as Gated Recurrent Unit (GRU). As we know that predicting stock prices is not an easy task to do, it requires extensive study of the stock market and intervention of statistical and machine learning models. We will try to spot whether the forecasting before pandemic is better than the forecasting during the pandemic for each of the six leading multinational tourism companies.