bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-COX-2 autoantibody is a novel biomarker of immune aplastic anemia
Authors: Kelkka Tiina , Tyster Mikko , Lundgren Sofie , Feng Xingmin , Kerr Cassandra , Hosokawa Kohei , Huuhtanen Jani , Keränen Mikko , Patel Bhavisha , Kawakami Toru , Maeda Yuka , Nieminen Otso , Kasanen Tiina , Aronen Pasi , Yadav Bhagwan , Rajala Hanna , Nakazawa Hideyuki , Jaatinen Taina , Hellström-Lindberg Eva , Ogawa Seishi , Ishida Fumihiro , Nishikawa Hiroyoshi , Nakao Shinji , Maciejewski Jaroslaw , Young Neal S. , Mustjoki Satu 
Published: August 2022

AbstractIn immune aplastic anemia (IAA), severe pancytopenia results from the immune-mediated destruction of hematopoietic stem cells. Several autoantibodies have been reported, but no clinically applicable autoantibody tests are available for IAA. We screened autoantibodies using a microarray containing >9000 proteins and validated the findings in a large international cohort of IAA patients (n = 405) and controls (n = 815). We identified a novel autoantibody that binds to the C-terminal end of cyclooxygenase 2 (COX-2, aCOX-2 Ab). In total, 37% of all adult IAA patients tested positive for aCOX-2 Ab, while only 1.7% of the controls were aCOX-2 Ab positive. Sporadic non-IAA aCOX-2 Ab positive cases were observed among patients with related bone marrow failure diseases, multiple sclerosis, and type I diabetes, whereas no aCOX-2 Ab seropositivity was detected in the healthy controls, in patients with non-autoinflammatory diseases or rheumatoid arthritis. In IAA, anti-COX-2 Ab positivity correlated with age and the HLA-DRB1*15:01 genotype. 83% of the >40 years old IAA patients with HLA-DRB1*15:01 were anti-COX-2 Ab positive, indicating an excellent sensitivity in this group. aCOX-2 Ab positive IAA patients also presented lower platelet counts. Our results suggest that aCOX-2 Ab defines a distinct subgroup of IAA and may serve as a valuable disease biomarker.
Human umbilical cord mesenchymal stem cells for psoriasis: a phase 1/2a, single-arm study
Authors: Cheng Lamei , Wang Siqi , Peng Cong , Zou Xiao , Yang Chao , Mei Hua , Li Chuang , Su Xian , Xiao Na , Ouyang Qi , Zhang Mi , Wang Qiaolin , Luo Yan , Shen Minxue , Qin Qun , Wang Honglin , Zhu Wu , Lu Guangxiu , Lin Ge , Kuang Yehong , Chen Xiang 
Published: August 2022

AbstractPsoriasis is a common, chronic immune-mediated systemic disease that had no effective and durable treatment. Mesenchymal stem cells (MSCs) have immunomodulatory properties. Therefore, we performed a phase 1/2a, single-arm clinical trial to evaluate the safety and efficacy of human umbilical cord-derived MSCs (UMSCs) in the treatment of psoriasis and to preliminarily explore the possible mechanisms. Seventeen patients with psoriasis were enrolled and received UMSC infusions. Adverse events, laboratory parameters, PASI, and PGA were analyzed. We did not observe obvious side effects during the treatment and 6-month follow-up. A total of 47.1% (8/17) of the psoriasis patients had at least 40% improvement in the PASI score, and 17.6% (3/17) had no sign of disease or minimal disease based on the PGA score. And the efficiency was 25% (2/8) for males and 66.7% (6/9) for females. After UMSC transplantation (UMSCT), the frequencies of Tregs and CD4+ memory T cells were significantly increased, and the frequencies of T helper (Th) 17 and CD4+ naive T cells were significantly decreased in peripheral blood (PB) of psoriasis patients. And all responders showed significant increases in Tregs and CD4+ memory T cells, and significant decreases in Th17 cells and serum IL-17 level after UMSCT. And baseline level of Tregs in responders were significantly lower than those in nonresponders. In conclusion, allogeneic UMSCT is safe and partially effective in psoriasis patients, and level of Tregs may be used as a potent biomarker to predict the clinical efficacy of UMSCT. Trial registration Clinical Trials NCT03765957
A temperature responsive adhesive hydrogel for fabrication of flexible electronic sensors
Authors: Liu Wan , Xie Ruijie , Zhu Jingyu , Wu Jiansheng , Hui Junfeng , Zheng Xiaoyan , Huo Fengwei , Fan Daidi 
Published: August 2022

AbstractFlexible electronics are playing an increasingly important role in human health monitoring and healthcare diagnosis. Strong adhesion on human tissue would be ideal for reducing interface resistance and motion artifacts, but arising problems such as skin irritation, rubefaction, and pain upon device removal have hampered their utility. Here, inspired by the temperature reversibility of hydrogen bonding, a skin-friendly conductive hydrogel with multiple-hydrogen bonds was designed by using biocompatible poly(vinyl alcohol) (PVA), phytic acid (PA), and gelatin (Gel). The obtained PVA/PA/Gel (PPG) hydrogel with temperature-triggered tunable mechanic could reliably adhere to skin and detect electrophysiological signals under a hot compress while be readily removed under a cool compress. Furthermore, the additional advantages of transparency, breathability, and antimicrobial activity of the PPG hydrogel ensure its long-time wearable value on the skin. It is both environmentally friendly and cost saving for the waste PPG hydrogel during production can be recycled based on their reversible physical bonding. The PPG hydrogel sensor is expected to have good application prospects to record electrophysiological signals in human health monitoring.
Degradation of thermal oxide film on pure titanium in an acidic environment containing fluoride
Authors: Wang Liwei , Wang Mingtao , Zhong Mingyuan , Li Xiaoqi , Cui Zhongyu 
Published: August 2022

AbstractElectrochemical degradation behavior of thermally oxidized pure Ti in the fluoride-containing acidic environments was investigated. Compact TiO2 oxides with acceptable hardness and superior corrosion resistance are formed through the thermal oxidation at 650 °C for 24 h. Corrosion of the oxidized Ti depends on the oxidation condition and fluoride content, resulting in the various interfacial structures at the oxide/solution and metal/solution interface. Degradation of the oxidized Ti consists of three stages during long-term immersion, while its critical fluoride concentration is increased by 1~2 orders of magnitude, attributed to the high stability of the TiO2 thermal oxides.
Specific gut microbiota alterations in essential tremor and its difference from Parkinson’s disease
Authors: Zhang Pingchen , Huang Pei , Du Juanjuan , He Yixi , Liu Jin , He Guiying , Cui Shishuang , Zhang Weishan , Li Gen , Chen Shengdi 
Published: August 2022

AbstractEssential tremor (ET) is the most common movement disorder and share overlapping symptoms with Parkinson’s disease (PD), making differential diagnosis challenging. Gut dysbiosis is regarded crucial in the pathogenesis of PD. Since ET patients also has comorbidity in gastrointestinal disorders, the relationship between gut microbiota and ET really worth investigating and may help distinguishing ET from PD. Fecal samples from 54 ET, 67 de novo PD and 54 normal controls (NC) were collected for 16S ribosomal RNA gene sequencing and quantitative real-time PCR. ET showed lower species richness (Chao1 index) than NC and PD. ET was withBacteroides-dominant enterotype, while PD was withRuminococcus-dominant enterotype. Compared with NC, 7 genera were significantly reduced in ET, 4 of which (Ruminococcus, Romboutsia, Mucispirillum,andAeromonas) were identified to be distinctive with an area under the curve (AUC) of 0.705. Compared to PD, 26 genera were found significantly different from ET, 4 of which (Bacteroides,Fusobacterium,Phascolarctobacterium,andLachnospira)were found distinguishable with an AUC of 0.756. Clinical association results indicated thatProteuswas associated with disease severity (TETRAS) of ET, whileKlebsiellawas linked to depression and anxiety in ET. Functional predictions revealed that 4 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were altered in ET. This study reveals gut dysbiosis in ET and it provides new insight into the pathogenesis of ET and helps distinguishing ET from PD.
Interfacial phase frustration stabilizes unconventional skyrmion crystals
Authors: Mohanta Narayan , Dagotto Elbio 
Published: August 2022

AbstractChiral magnetic phases with an unconventional topological twist in the magnetization are of huge interest due to their potential in spintronics applications. Here, we present a general method to induce such exotic magnetic phases using interfacial phase frustration within artificially grown superlattices. To demonstrate our method, we consider a multilayer with two different chiral magnetic phases as the competing orders at the top and bottom and show, using Monte Carlo calculations, that the interfacial phase frustration is realized at the central layer. In particular, we obtain three unconventional phases: a checkerboard skyrmion crystal, an incommensurate skyrmion stripe, and a ferrimagnetic skyrmion crystal. In these frustration-induced phases, the spin chirality-driven topological Hall conductivity can be largely enhanced. This method provides a playground to realize unconventional magnetic phases in any family of materials that can be grown in superlattices.
Language specificity in cortical tracking of speech rhythm at the mora, syllable, and foot levels
Authors: Peter Varghese , van Ommen Sandrien , Kalashnikova Marina , Mazuka Reiko , Nazzi Thierry , Burnham Denis 
Published: August 2022

AbstractRecent research shows that adults’ neural oscillations track the rhythm of the speech signal. However, the extent to which this tracking is driven by the acoustics of the signal, or by language-specific processing remains unknown. Here adult native listeners of three rhythmically different languages (English, French, Japanese) were compared on their cortical tracking of speech envelopes synthesized in their three native languages, which allowed for coding at each of the three language’s dominant rhythmic unit, respectively the foot (2.5 Hz), syllable (5 Hz), or mora (10 Hz) level. The three language groups were also tested with a sequence in a non-native language, Polish, and a non-speech vocoded equivalent, to investigate possible differential speech/nonspeech processing. The results first showed that cortical tracking was most prominent at 5 Hz (syllable rate) for all three groups, but the French listeners showed enhanced tracking at 5 Hz compared to the English and the Japanese groups. Second, across groups, there were no differences in responses for speech versus non-speech at 5 Hz (syllable rate), but there was better tracking for speech than for non-speech at 10 Hz (not the syllable rate). Together these results provide evidence for both language-general and language-specific influences on cortical tracking.
Influence of organic ammonium derivatives on the equilibria between NH4+, NO2− and NO3− ions in the Nistru River water
Authors: Spataru Petru 
Published: August 2022

AbstractThe toxic effects of ammonium derivatives in the river water depend dramatically on their natural or synthetic origins and on their chemical structures. It has been proved that 1-naphtylamine (1-NA) and diphenylamine (DPA) breaking impact on the ammonium oxidation and especially on nitrite ions oxidation processes in natural waters is associated with its toxicity. The NH4+ oxidation process slows down for about five days and ten days in river water samples with 0.5 mg/L DPA and corresponding 0.5 mg/L 1-NA. The NO2− oxidation delay in model samples of river water with 0.025 and 0.05 mg/L 1-NA, is four days and 35 days in the one with 0.5 mg/L 1-NA. For the sample with 0.05 mg/L DPA the delay of the NO2− oxidation is approximately of six days and 25 days for sample with 0.5 mg/L, DPA. The laboratory simulations have revealed: (1) absorption–desorption, the micro biotic reaction to the instantaneous increase of the concentration of ammonium ion in the river water (so-called shock/stress effect) and (2) the NH4+ increase stimulated by a certain (0.05 mg/L) concentration of 1-NA.The diethylamine (DEA) decomposition leads to increasing with approximately 3.8 mg/L NH4+ in river water samples of 20.0 mg/L DEA.
Epidemiological features of tuberculosis infection in a rural prefecture of Japan from 2007 to 2018
Authors: Lu Yixiao , Cai Guoxi , Liu Yuhang , He Fei , Aoyagi Kiyoshi 
Published: August 2022

AbstractThis study aimed to investigate the epidemiological features of reported tuberculosis (TB) infections in a western prefecture (Nagasaki Prefecture) from 2007 to 2018, and to identify the high-risk group for TB infection. The characteristics of 12 years of reported TB infections from the Nagasaki Prefectural Informational Center of Infectious Diseases were summarized by median (interquartile range [IQR]) and proportion; the annual TB infections’ notification rate regarding sex/age was calculated accordingly. The diagnosis of TB infection was made according to clinic symptoms and laboratory examination. In total, 4364 TB infections were reported in 2007 and 2018, with a median age (IQR) of 74 (55–84) years. The majority of TB infections were male (52.6%, 2297/4364), > 65 years (65.8%, 2869/4364), and indigenous (98.1%, 4276/4364). Among active TB, 66.9% (1833/2740) had pulmonary TB, and 25.3% (694/2740) were diagnosed as extrapulmonary TB. The highest notification rate of TB infection was observed in the elderly male population (> 85 years). The annual notification rate of TB infections ranged between 19.4/and 34.0/100,000 for 12 years. The notification rates of TB infections were high in older people of both sexes, especially in men aged > 85. Therefore, appropriate interventions and health management are essential for TB control in (and with a focus on) the elderly population.
Non-cystic macular thickening on optical coherence tomography as an alternative to fluorescein angiography for predicting retinal vascular leakage in early stages of uveitis
Authors: Ebrahimiadib Nazanin , Kianzad Zahra , Zarei Mohammad , Davoudi Samaneh , Riazi-Esfahani Hamid , Bazvand Fatemeh , Mahdizad Zahra , Modjtahedi Bobeck S. 
Published: August 2022

AbstractTo evaluate the relationship between non-cystic thickening of the macula on optical coherence tomography (OCT) and retinal vascular leakage on fluorescein angiogram (FA) in patients with uveitis. A cross-sectional study of patients seen in the uveitis clinic. Patients with any degree of inflammatory cells in the anterior vitreous were included, provided that no macular cyst or subretinal fluid or macular atrophy was observed in OCT. The correlation between OCT features and best corrected visual acuity (BCVA), the degree of inflammation, and FA findings were examined. The severity of vascular leakage in FA was graded for optic nerve, macula and posterior and peripheral leakage. We used generalized estimation equation to assess the associations between macular thickness and volume with angiographic scores. A total of 43 patients (100 exam data) met inclusion criteria. There was a significant relationship between OCT parameters (central macular thickness, 3 mm and 6 mm perifoveal macular thickness as well as total and central macular volume) with angiographic scores (macular, optic disc, posterior and peripheral vascular leakage score) (all P values < 0.0001). The correlation between the 6 mm perifoveal thickness and peripheral vascular leakage score (R = 0.76; P < 0.001) was stronger than the correlation of CMT with this angiographic score (R = 0.69; P < 0.001). Non-cystic thickening of the macula on OCT, especially in perifoveal area, is a reliable predictor of the presence of retinal vascular leakage in patients with uveitis.
Staphylococcus aureus lineages associated with a free-ranging population of the fruit bat Pteropus livingstonii retained over 25 years in captivity
Authors: Fountain Kay , Barbon Alberto , Gibbon Marjorie J. , Lloyd David H. , Loeffler Anette , Feil Edward J. 
Published: August 2022

AbstractConservation of endangered species has become increasingly complex, and costly interventions to protect wildlife require a robust scientific evidence base. This includes consideration of the role of the microbiome in preserving animal health. Captivity introduces stressors not encountered in the wild including environmental factors and exposure to exotic species, humans and antimicrobial drugs. These stressors may perturb the microbiomes of wild animals, with negative consequences for their health and welfare and hence the success of the conservation project, and ultimately the risk of release of non-native organisms into native ecosystems. We compared the genomes of Staphylococcus aureus colonising critically endangered Livingstone’s fruit bats (Pteropus livingstonii) which have been in a captive breeding programme for 25 years, with those from bats in the endemic founder population free ranging in the Comoros Republic. Using whole genome sequencing, we compared 47 isolates from captive bats with 37 isolates from those free ranging in the Comoros Republic. Our findings demonstrate unexpected resilience in the bacteria carried, with the captive bats largely retaining the same two distinctive lineages carried at the time of capture. In addition, we found evidence of genomic changes which suggest specific adaptations to the bat host.
Cell-based receptor discovery identifies host factors specifically targeted by the SARS CoV-2 spike
Authors: Husain Bushra , Yuen Kobe , Sun Dawei , Cao Shengya , Payandeh Jian , Martinez-Martin Nadia 
Published: August 2022

AbstractReceptor-ligand interactions on the plasma membrane regulate cellular communication and play a key role in viral infection. Despite representing main targets for drug development, the characterization of these interactions remains challenging in part due to the dearth of optimal technologies. Here, we build a comprehensive library of human proteins engineered for controlled cell surface expression. Coupled to tetramer-based screening for increased binding avidity, we develop a high throughput cell-based platform that enables systematic interrogation of receptor-ligand interactomes. Using this technology, we characterize the cell surface proteins targeted by the receptor binding domain (RBD) of the SARS-CoV spike protein. Host factors that specifically bind to SARS CoV-2 but not SARS CoV RBD are identified, including proteins that are expressed in the nervous system or olfactory epithelium. Remarkably, our results show that Contactin-1, a previously unknown SARS CoV-2 spike-specific receptor that is upregulated in COVID-19 patients, significantly enhances ACE2-dependent pseudotyped virus infection. Starting from a versatile platform to characterize cell surface interactomes, this study uncovers host factors specifically targeted by SARS CoV-2, information that may help design improved therapeutic strategies against COVID-19.
Conformational flexibility in neutralization of SARS-CoV-2 by naturally elicited anti-SARS-CoV-2 antibodies
Authors: Li Ruofan , Mor Michael , Ma Bingting , Clark Alex E. , Alter Joel , Werbner Michal , Lee Jamie Casey , Leibel Sandra L. , Carlin Aaron F. , Dessau Moshe , Gal-Tanamy Meital , Croker Ben A. , Xiang Ye , Freund Natalia T. 
Published: August 2022

AbstractAs new variants of SARS-CoV-2 continue to emerge, it is important to assess the cross-neutralizing capabilities of antibodies naturally elicited during wild type SARS-CoV-2 infection. In the present study, we evaluate the activity of nine anti-SARS-CoV-2 monoclonal antibodies (mAbs), previously isolated from convalescent donors infected with the Wuhan-Hu-1 strain, against the SARS-CoV-2 variants of concern (VOC) Alpha, Beta, Gamma, Delta and Omicron. By testing an array of mutated spike receptor binding domain (RBD) proteins, cell-expressed spike proteins from VOCs, and neutralization of SARS-CoV-2 VOCs as pseudoviruses, or as the authentic viruses in culture, we show that mAbs directed against the ACE2 binding site (ACE2bs) are more sensitive to viral evolution compared to anti-RBD non-ACE2bs mAbs, two of which retain their potency against all VOCs tested. At the second part of our study, we reveal the neutralization mechanisms at high molecular resolution of two anti-SARS-CoV-2 neutralizing mAbs by structural characterization. We solve the structures of the Delta-neutralizing ACE2bs mAb TAU-2303 with the SARS-CoV-2 spike trimer and RBD at 4.5 Å and 2.42 Å resolutions, respectively, revealing a similar mode of binding to that between the RBD and ACE2. Furthermore, we provide five additional structures (at resolutions of 4.7 Å, 7.3 Å, 6.4 Å, 3.3 Å, and 6.1 Å) of a second antibody, TAU-2212, complexed with the SARS-CoV-2 spike trimer. TAU-2212 binds an exclusively quaternary epitope, and exhibits a unique, flexible mode of neutralization that involves transitioning between five different conformations, with both arms of the antibody recruited for cross linking intra- and inter-spike RBD subunits. Our study provides additional mechanistic understanding about how antibodies neutralize SARS-CoV-2 and its emerging variants and provides insights on the likelihood of reinfections.
Red light-induced conjugation of amines through amide bond formation triggered via photooxidation of 3-acylindolizines
Authors: Watanabe Kenji , Kuratsu Asuka , Hashizume Daisuke , Niwa Takashi , Hosoya Takamitsu 
Published: August 2022

AbstractThe development of a conjugation method initiated by irradiation of long-wavelength light (>500 nm) to prepare densely functionalized molecules while avoiding undesired photodegradation has attracted considerable attention. Here we show an amide bond formation method based on the photoreaction of 3-acylindolizines in the presence of amines triggered via red-light irradiation. Photooxidation of 3-acylindolizines using a catalytic amount of a photosensitizer and red light-emitting diodes (660 nm) affords the corresponding conjugated amides in nearly quantitative yields within <5 min. This transformation can be performed in aqueous organic solvents and is applicable to diverse aliphatic amines with various functional groups, including the moieties responsive to short-wavelength light.
Reservoir computing on a silicon platform with a ferroelectric field-effect transistor
Authors: Toprasertpong Kasidit , Nako Eishin , Wang Zeyu , Nakane Ryosho , Takenaka Mitsuru , Takagi Shinichi 
Published: August 2022

AbstractReservoir computing offers efficient processing of time-series data with exceptionally low training cost for real-time computing in edge devices where energy and hardware resources are limited. Here, we report reservoir computing hardware based on a ferroelectric field-effect transistor (FeFET) consisting of silicon and ferroelectric hafnium zirconium oxide. The rich dynamics originating from the ferroelectric polarization dynamics and polarization-charge coupling are the keys leading to the essential properties for reservoir computing: the short-term memory and high-dimensional nonlinear transform function. We demonstrate that an FeFET-based reservoir computing system can successfully solve computational tasks on time-series data processing including nonlinear time series prediction after training with simple regression. Due to the FeFET’s high feasibility of implementation on the silicon platform, the systems have flexibility in both device- and circuit-level designs, and have a high potential for on-chip integration with existing computing technologies towards the realization of advanced intelligent systems.