bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accelerated River Meander Migration on the Tibetan Plateau Caused by Permafrost Thaw
Authors: Sha Anmeng , Li Dongfeng , Walling Des , Zhao Yi , Tian Shang , Chen Dong , Deng Shanshan , Xia Junqiang , Best Jim 
Published: January 2025

AbstractThe migration of rivers in permafrost landscapes has critical implications for riverine infrastructure, ecosystem stability, and carbon cycling, yet its magnitude and underlying mechanisms remain poorly understood. Here, we leverage four decadal satellite imagery, hydrological observations, and permafrost modeling to investigate meander migration dynamics on the Tibetan Plateau. Our data show that the migration rates of permafrost rivers have increased by 34.6% from 1987 to 2022, in response to the combined effects of increased discharge, riverbank destabilization driven by ground ice melt and extended thawing days (increased by 35 days). In contrast, rivers flowing across seasonally frozen ground exhibited a decline in migration rate by 11.1%, driven by vegetation greening and riverbank stabilization. In a future warming climate for the Tibetan Plateau, the migration rates of permafrost rivers are anticipated to further accelerate, potentially threatening riverine infrastructure safety and aquatic ecosystems, and intensifying the permafrost carbon cycle.
Biogeophysical Radiative Forcings of Large‐Scale Afforestation in Europe Are Highly Localized and Dominated by Surface Albedo Change
Authors: Bright Ryan M. , Caporaso Luca , Duveiller Gregory , Piccardo Matteo , Cescatti Alessandro 
Published: January 2025

AbstractLarge‐scale re‐/afforestation projects afford sizable atmospheric CO2 removals yet questions loom surrounding their potentially offsetting biogeophysical radiative forcings. Forest area change alters not only the surface albedo but also heat, moisture, and momentum fluxes, which in turn modify the atmosphere's radiative, thermodynamical, and dynamical properties. These so‐called radiative forcing “adjustments” have been little examined in re‐/afforestation contexts, and many questions remain surrounding their relevance in relation to the instantaneous forcing from the surface albedo change—and whether they can affect Earth's radiative energy balance in regions remote from where the re‐/afforestation occurs. Here, we quantified biogeophysical radiative forcings and adjustments from realistically scaled re‐/afforestation in Europe at high spatial resolution and found that adjustments with high signal‐to‐noise were largely confined to only a few months and to the region of re‐/afforestation. Adjustments were dominated by perturbed low‐level clouds and rarely exceeded ±25% of the annual albedo change forcing.
Accelerated Permafrost Thaw Linked to Rising River Temperature and Widening Channels
Authors: Zhao Yi , Li Dongfeng 
Published: January 2025

AbstractRiver‐controlled permafrost dynamics are crucial for sediment transport, infrastructure stability, and carbon cycle, yet are not well understood under climate change. Leveraging remotely sensed datasets, in‐situ hydrological observations, and physics‐based models, we reveal overall warming and widening rivers across the Tibetan Plateau in recent decades, driving accelerated sub‐river permafrost thaw. River temperature of a representative section (Tuotuohe River) on the central Tibetan Plateau, has increased notably (0.39°C/decade) from 1985 to 2017, facilitating heat transfer into the underlying permafrost via both convection and conduction. Consequently, the permafrost beneath rivers warms faster (0.37°C–0.66°C/decade) and has a ∼0.5 m thicker active layer than non‐inundated permafrost (0.17°C–0.49°C/decade). With increasing river discharge, the inundated area expands laterally along the riverbed (16.4 m/decade), further accelerating permafrost thaw for previously non‐inundated bars. Under future warmer and wetter climate, the anticipated intensification of sub‐river permafrost degradation will pose risks to riverine infrastructure and amplify permafrost carbon release.
Investigations on Bore Capture Modulation on a Macrotidal Beach
Authors: Hart R. M. , Blenkinsopp C. , Stringari C. E. , Power H. E. 
Published: January 2025

AbstractThe ability to empirically and accurately predict runup on natural beaches is made difficult by the random nature of waves and extreme runup events. In some cases, extreme runup events are the result of bore capture where one broken wave passes over the front of and merges with another broken wave or shoreline capture where capture occurs at the instantaneous shoreline. Here we use high resolution Lidar data to investigate potential drivers of bore and shoreline capture on a macro‐tidal dissipative beach. The proportion of runup events that are derived from capture(s) was identified within normalized runup elevation percentiles which increased from 15% in the lowest tenth percentile of runup elevations to 55% of runup events in the highest tenth percentile. Bore capture was found to occur primarily on the rising infragravity wave in both space and time, whereas shoreline capture occurred predominately during the rising and peak phases of the infragravity wave. The occurrence of bore capture was not, however, fully restricted to particular infragravity phases, suggesting multiple drivers of capture. Bore trajectories of pairs of captured bores and non‐captured bores were tracked showing that the probability of capture is also a function of normalized interwave proximity, the ratio of depths beneath consecutive wave crests, and normalized proximity to the mean shoreline. More dissipative beaches therefore not only have more infragravity energy within the surf and swash zones (thus infragravity modulation of bore capture), the wider surf zones provides greater time for bores to capture preceding bores.
Latitudinal Trends in Drivers of the Southern Ocean Spring Bloom Onset
Authors: Schlosser Tamara L. , Strutton Peter G. , Baker Kirralee , Boyd Philip W. 
Published: January 2025

AbstractThe Southern Ocean spring phytoplankton bloom impacts regional food webs and the marine carbon cycle, but we do not fully understand which drivers—environmental, ecological, or biological—control the timing of the onset of the spring bloom. Nutrients, particularly iron, are likely replete in the austral winter, but the importance of underwater light availability and grazing pressure are topics of ongoing discussion. Furthermore, in the extreme polar winter, phytoplankton physiology may impart additional constraints on the bloom onset. We analyzed biogeochemical (BGC) Argo profiles from the Pacific sector of the Southern Ocean, and a one‐dimensional water column turbulence model forced by reanalysis data. Though the surface mixed layer defines where density is homogenous, the presence of enhanced turbulence and the active mixing of constituents, such as chlorophyll fluorescence, is better estimated by the depth of active mixing that we estimate from the turbulence model. We identified two regimes: one north of the subantarctic front where bloom onsets occur around July, before the seasonal maximum in mixing depth and when light availability remained near its annual minimum value. It is likely that changes in the phytoplankton loss rate control the bloom onset in this region. South of the subantarctic front, bloom onsets occur closer to austral spring following enhanced light availability, suggesting that bloom onset is primarily controlled by phytoplankton growth rather than loss terms. Our analysis shows that new insights can be gained into spring bloom phenology from the combination of float and model data.
Dynamics of Near‐Bottom Currents in Cold‐Water Coral and Sponge Areas at Valdivia Bank and Ewing Seamount, Southeast Atlantic
Authors: Mohn Christian , Schwarzkopf Franziska U. , García Patricia Jiménez , Orejas Covadonga , Huvenne Veerle A. I. , Schumacher Mia , Pérez‐Rodríguez Irene , Sarralde Vizuete Roberto , López‐Abellán Luis J. , Dale Andrew C. , Devey Colin , Hansen Jørgen L. S. , Møller Eva Friis , Biastoch Arne 
Published: January 2025

AbstractThis study investigates near‐bottom currents and physical processes from simulations with the hydrodynamic model ROMS‐AGRIF at two seamounts of the northeast Walvis Ridge to obtain valuable insights about drivers of observed occurrences of benthic suspension feeders (cnidarians and sponges) in this data‐poor area. The spatial resolution in each model area was increased across two levels of nested grids from 1,500 m to 500 m resolution with 32 stretched terrain‐following (s‐) layers in the vertical with high resolution close to the bottom. The parent grids receive initial and boundary conditions from the basin‐scale model INALT20 and from solutions of the OTIS inverse tidal model. The model topography is based on GEBCO data with local refinements from multi‐beam data collected during different surveys in 2008, 2009, and 2010. Increasing model resolution is an important advancement for precisely evaluating the intrinsic dynamics within challenging rough terrain. We examined how near‐bottom currents vary over space and time and investigated potential links between observed Cnidarian and Porifera occurrences and ranges of physical variables and processes. We identified a close link between physical processes and species distributions and suggested that physical processes such as kinetic energy dissipation and internal wave dynamics may be considered in future research as proxies of food supply to benthic suspension feeders. Such mechanistic variables may also be used to supplement more traditional descriptors such as water mass and terrain properties in species distribution models, thus enhancing our ability to predict the occurrence of benthic communities characterized by cnidarians and sponges.
A pan-immune panorama of bacterial pneumonia revealed by a large-scale single-cell transcriptome atlas
Authors: Xiao Kun , Cao Yan , Han Zhihai , Zhang Yuxiang , Luu Laurence Don Wai , Chen Liang , Yan Peng , Chen Wei , Wang Jiaxing , Liang Ying , Shi Xin , Wang Xiuli , Wang Fan , Hu Ye , Wen Zhengjun , Chen Yong , Yang Yuwei , Yu Haotian , Xie Lixin , Wang Yi 
Published: January 2025

AbstractBacterial pneumonia is a significant public health burden, contributing to substantial morbidity, mortality, and healthcare costs. Current therapeutic strategies beyond antibiotics and adjuvant therapies are limited, highlighting the need for a deeper understanding of the disease pathogenesis. Here, we employed single-cell RNA sequencing of 444,146 bronchoalveolar lavage fluid cells (BALFs) from a large cohort of 74 individuals, including 58 patients with mild (n = 22) and severe (n = 36) diseases as well as 16 healthy donors. Enzyme‐linked immunosorbent and histological assays were applied for validation within this cohort. The heterogeneity of immune responses in bacterial pneumonia was observed, with distinct immune cell profiles related to disease severity. Severe bacterial pneumonia was marked by an inflammatory cytokine storm resulting from systemic upregulation of S100A8/A9 and CXCL8, primarily due to specific macrophage and neutrophil subsets. In contrast, mild bacterial pneumonia exhibits an effective humoral immune response characterized by the expansion of T follicular helper and T helper 2 cells, facilitating B cell activation and antibody production. Although both disease groups display T cell exhaustion, mild cases maintained robust cytotoxic CD8+T cell function, potentially reflecting a compensatory mechanism. Dysregulated neutrophil and macrophage responses contributed significantly to the pathogenesis of severe disease. Immature neutrophils promote excessive inflammation and suppress T cell activation, while a specific macrophage subset (Macro_03_M1) displaying features akin to myeloid-derived suppressor cells (M-MDSCs) suppress T cells and promote inflammation. Together, these findings highlight potential therapeutic targets for modulating immune responses and improving clinical outcomes in bacterial pneumonia.
Association of novel ERLIN2 gene variants with hereditary spastic paraplegia
Authors: Bermejo Ramírez R. , Villena Gascó N. , Ruiz Palmero L. , Ribes Bueno G. A. , Yamanaka E. S. , Piqueras Flores J. , Flores Barragán J. M. , Buces González E. , Arroyo Andújar J. D. 
Published: January 2025

AbstractTwo ERLIN2 variants (NM_007175.8:c.660delA and NM_007175.8:c.869C>T) were detected in a Spanish patient with hereditary spastic paraplegia via whole-exome sequencing and software-based pathogenic variant selection. Segregation analysis revealed that the patient’s two affected siblings carried both variants, whereas their offspring, carrying only one variant, were asymptomatic, indicating the autosomal recessive nature of the disease. These findings suggest that the identified variants can be classified as pathogenic when they are present as compound heterozygous variants.
Clinical characteristics of the Ala21Val variant in the myelin proteolipid protein 1 (PLP1) gene associated with Pelizaeus-Merzbacher disease in a Brazilian male patient
Authors: Manzke Pedro , Brandão Pedro Renato P. , Balieiro Talita , de Carvalho Bispo Diógenes Diego , Osório Maria Joana , Barra Gustavo Barcelos 
Published: January 2025

AbstractHere, we report the case of a 29-year-old male with classic Pelizaeus-Merzbacher disease (PMD) harboring the PLP1 variant NM_000533.5:c.62 C > T, leading to an NP_000524.3:p.(Ala21Val) alteration in the first transmembrane domain of the protein. He presented with developmental delays, nystagmus, spastic paraparesis, optic atrophy, dysphagia, appendicular ataxia, and progressive head tremor. Brain MRI revealed hypomyelination, diffuse white matter hyperintensity, and atrophy of the corpus callosum and cerebellum, expanding the known clinical spectrum of PMD.
A novel UBA1 gene mutation in a patient with infantile respiratory distress syndrome
Authors: Miyata Masafumi , Kojima Arisa , Kawai Yuri , Uchida Hidetoshi , Boda Hiroko , Ishihara Naoko , Inagaki Hidehito , Yoshikawa Tetsushi , Kurahashi Hiroki 
Published: January 2025

AbstractUBA1 is an E1 ubiquitin-activating enzyme that initiates the ubiquitylation of target proteins and is thus a key component of the ubiquitin signaling pathway. Three disorders are associated with pathogenic variants of the UBA1 gene: vacuoles, E1 enzyme, X-linked, autoinflammatory, somatic (VEXAS) syndrome, lung cancer in never smokers (LCINS), and X-linked spinal muscular atrophy (XL-SMA, SMAX2). We here report a case of infantile respiratory distress syndrome followed by continuing neuromuscular symptoms. We identified a de novo hemizygous mutation, c.1660 C > T (p.Pro554Ser), in exon 15 of the UBA1 gene in this baby. This missense mutation was located with the AAD (active adenylation domain) of the protein, a known hotspot of SMAX2 mutations. This case lends support to the genotype-phenotype correlation regarding the UBA1 mutation and its related diseases.
Parasitic structure defect blights sustainability of cobalt-free single crystalline cathodes
Authors: Yu Lei , Dai Alvin , Zhou Tao , Huang Weiyuan , Wang Jing , Li Tianyi , He Xinyou , Ma Lu , Xiao Xianghui , Ge Mingyuan , Amine Rachid , Ehrlich Steven N. , Ou Xing , Wen Jianguo , Liu Tongchao , Amine Khalil 
Published: January 2025

AbstractRecent efforts to reduce battery costs and enhance sustainability have focused on eliminating Cobalt (Co) from cathode materials. While Co-free designs have shown notable success in polycrystalline cathodes, their impact on single crystalline (SC) cathodes remains less understood due to the significantly extended lithium diffusion pathways and the higher-temperature synthesis involved. Here, we reveal that removing Co from SC cathodes is structurally and electrochemically unfavorable, exhibiting unusual voltage fade behavior. Using multiscale diffraction and imaging techniques, we identify lithium-rich nanodomains (LRNDs) as a heterogeneous phase within the layered structure of Co-free SC cathodes. These LRNDs act as critical tipping points, inducing significant chemo-mechanical lattice strain and irreversible structural degradation, which exacerbates the voltage and capacity loss in electrochemical performance. Our findings highlight the considerable challenges of developing Co-free SC cathodes compared to polycrystalline ones and emphasize the need for new strategies to balance the interplay between cost, sustainability, and performance.
Steroid hormone-induced wingless ligands tune female intestinal size in Drosophila
Authors: Zipper Lisa , Corominas-Murtra Bernat , Reiff Tobias 
Published: January 2025

AbstractFemale reproduction comes at great expense to energy metabolism compensated by extensive organ adaptations including intestinal size. Upon mating, endocrine signals orchestrate a 30% net increase of absorptive epithelium. Mating increases production of the steroid hormone Ecdysone released by the Drosophila ovaries that stimulates intestinal stem cell (ISC) divisions. Here, we uncover the transcription factor crooked legs (crol) as an intraepithelial coordinator of Ecdysone-induced ISC mitosis. For the precise investigation of non-autonomous factors on ISC behaviour, we establish Rapport, a spatiotemporally-controlled dual expression and tracing system for the analysis of paracrine genetic manipulation while tracing ISC behaviour. Rapport tracing reveals that Ecdysone-induced Crol controls mitogenic Wnt/Wg-ligand expression from epithelial enterocytes activating ISC mitosis. Paracrine Wg stimulation is counterbalanced by Crol-repression of string/CDC25 and CyclinB autonomously in ISC. Rapport-based ISC tumours confirm paracrine stimulation through the Ecdysone-Crol-Wg axis on mitotic behaviour, whereas the autonomous anti-proliferative role of Crol in ISC is conserved in models of colorectal cancer. Finally, mathematical modelling corroborates increasing enterocyte numbers and Wnt/Wg-degradation to set a stable post-mating intestinal size. Together, our findings provide insights into the complex endocrine growth control mechanisms during mating-induced adaptations and might help untangling pleiotropic hormonal effects observed in gastrointestinal tumorigenesis.
Targeting mutant p53: Evaluation of novel anti-p53R175H monoclonal antibodies as diagnostic tools
Authors: Spiegelberg Diana , Hwang Le-Ann , Pua Khian Hong , Kumar Sashwini Chandra , Koh Xin Yu , Koh Xiao Hui , Selvaraju Ram Kumar , Sabapathy Kanaga , Nestor Marika , Lane David 
Published: January 2025

AbstractAbout 50% of all cancers carry a mutation in p53 that impairs its tumor suppressor function. The p53 missense mutation p53R175H (p53R172H in mice) is a hotspot mutation in various cancer types. Therefore, monoclonal antibodies selectively targeting clinically relevant mutations like p53R175H could prove immensely value. We aimed to evaluate the in vitro and in vivo binding properties of two novel anti-p53R175H monoclonal antibodies and to assess their performance as agents for molecular imaging. In vitro, 125I-4H5 and 125I-7B9 demonstrated long shelf life and antigen-specific binding. Our in vivo study design allowed head-to-head comparison of the antibodies in a double tumor model using repeated SPECT/CT imaging, followed by biodistribution and autoradiography. Both tracers performed similarly, with marginally faster blood clearance for 125I-7B9. Repeated molecular imaging demonstrated suitable imaging characteristics for both antibodies, with the best contrast images occurring at 48 h post-injection. Significantly higher uptake was detected in the mut-p53-expressing tumors, confirmed by ex vivo autoradiography. We conclude that molecular imaging with an anti-p53R175H tracer could be a promising approach for cancer diagnostics and could be further applied for patient stratification and treatment response monitoring of mutant p53-targeted therapeutics.
CAR T-cell-associated secondary malignancies challenge current pharmacovigilance concepts
Authors: Berg Philipp , Ruppert-Seipp Gabriele , Müller Susanne , Maurer Gabriele D , Hartmann Jessica , Holtick Udo , Buchholz Christian J , Funk Markus B 
Published: January 2025

Abstract
Suspected adverse reactions following chimeric antigen receptor T-cell (CAR T) treatment include more and more cases of secondary T-cell malignancies. The causality assessment of such suspected reactions challenges established evaluation practices due to (i) patient and product-specific risk factors and (ii) incomplete data available with post-marketing reports submitted to competent authorities. This is of particular relevance for gene therapy products that integrate into the host genome. We present a summary of case reports related to different CAR T products and the rationale for case causality assessment. In this context, possible pathophysiologic mechanisms and differences between CAR T products to be taken into account are discussed. The unparalleled complexity of the case follow-up and the multistep process of cancer development necessitates a case-by-case consideration. This highlights challenges in the pharmacovigilance of advanced therapy medicinal products and underlines the importance of testing for vector presence, integration location and gene expression profile for an informed case assessment of suspected secondary malignancies with the aim to obtain a better understanding of contributing factors.
Mandarin Chinese Translation and Cultural Adaptation of the Caregiver Analysis of Reported Experiences With Swallowing Disorders Screening Tool
Authors: Zhao Lancai , Li Sihan , Qiu Yufeng , Zhu Xueqiong , Shao Jing , Zhang Huafang 
Published: January 2025

Purpose:
The caregiver burden of individuals with dysphagia is a major concern. Currently, assessment tools specifically designed for this population are lacking. The present study aimed to translate the Caregiver Analysis of Reported Experiences with Swallowing Disorders (CARES) Questionnaire into Mandarin Chinese and evaluate its psychometric properties.

Method:
This study analyzed the psychometric properties of the Mandarin Chinese version of the CARES questionnaire using classical measurement theory and Rasch model analysis.

Results:

Classical measurement theory: The item-level content validity index of the Mandarin Chinese version of the CARES questionnaire ranged from .83 to 1.00, and the scale-level content validity index ranged from .93 to .95. The correlation coefficient between the total scores, subscale scores of the CARES questionnaire, and Zarit Caregiver Burden Interview scores was between .82 and .87 (
p
< .01). There were significant relationships between dysphagia-specific burden (CARES) and perceived swallowing impairment (Eating Assessment Tool-10) and diet restrictiveness (International Dysphagia Diet Standardisation Initiative Functional Diet Scale). The overall Cronbach's α coefficient of the Mandarin Chinese version of the CARES questionnaire was .81. Item response theory: The Mandarin Chinese version of the CARES questionnaire was unidimensional. The item difficulty and individual ability were evenly distributed. The total item reliability was .96, the person reliability was .79, the item separation index was 4.95, and the person separation index was 1.93.

Conclusion:
The Mandarin Chinese version of the CARES questionnaire demonstrates satisfactory reliability and validity and can be utilized as a specific assessment tool for evaluating the informal caregiver burden of individuals with dysphagia.