bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neighborhood-based Inference and Restricted Boltzmann Machine for microbe and drug associations prediction
Authors: Cheng Xiao-Long , Qu Jia , Ni Tong-Guang , Song Shuang-Bao , Bian Ze-Kang 
Published: April 2022

Abstract
Efficient identification of microbe-drug associations is critical for drug development and solving problem of antimicrobial resistance. Traditional wet-lab method requires a lot of money and labor in identifying potential microbe-drug associations. With development of machine learning and large amounts of biological data, computational methods become feasible. In this paper, we proposed a computational model of Neighborhood-based Inference (NI) and Restricted Boltzmann Machine (RBM) to predict potential Microbe-Drug Association (NIRBMMDA) by using multisource data. First, NI was used to predict potential microbe-drug associations by using different thresholds to find similar neighbors for drug or microbe. Then, RBM was also employed to predict potential microbe-drug associations based on contrastive divergence algorithm and sigmoid function. Because generalization ability of individual method is poor, we used an ensemble learning to integrate the two predicted microbe-drug associations. Especially, NI can fully utilize similar (neighbor) information of drug or microbe and RBM can learn potential probability distribution hid in known microbe-drug associations. Finally, ensemble learning was used to integrate individual predictor for obtaining a stronger predictor. To evaluate the performance of NIRBMMDA, global leave-one-out cross validation (LOOCV), local LOOCV and five-fold cross validations were implemented to evaluate the performance of NIRBMMDA based on three datasets of DrugVirus, MDAD and aBiofilm. In global LOOCV, NIRBMMDA gained the area under the receiver operating characteristics curve (AUC) of 0.8666, 0.9413 and 0.9557 for datasets of DrugVirus, MDAD and aBiofilm, respectively. In local LOOCV, AUCs of 0.8512, 0.9204 and 0.9414 were obtained for NIRBMMDA based on datasets of DrugVirus, MDAD and aBiofilm, respectively. For five-fold cross validation, NIRBMMDA acquired AUC and standard deviation of 0.8569 0.0027, 0.9248 0.0014 and 0.9369 0.0020 on the basis of datasets of DrugVirus, MDAD and aBiofilm, respectively. Moreover, case study for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) showed that 13 out of the top 20 predicted drugs were verified by searching literature. The other two case studies indicated that 17 and 15 out of the top 20 predicted microbes for the drug of ciprofloxacin and minocycline were confirmed by published literature, respectively. The results demonstrated NIRBMMDA is an effective model in predicting microbe-drug associations.
Nausea at the start of specialized palliative care and change in nausea after the first weeks of palliative care were associated with cancer site, gender and type of palliative care service - a nationwide study
Authors: Hansen Maiken Bang , Adsersen Mathilde , Rojas-Concha Leslye , Petersen Morten Aagaard , Ross Lone , Grønvold Mogens 
Published: April 2022

Abstract
Purpose
Nausea is a common and distressful symptom among patients in palliative care, but little is known about possible socio-demographic and clinical patient characteristics associated with nausea at the start of palliative care and change after initiation of palliative care. The aim of this study was to investigate whether patient characteristics were associated with nausea at the start of palliative care and with change in nausea during the first weeks of palliative care, respectively.
Methods
Data was obtained from the nationwide Danish Palliative Care Database. The study included adult cancer patients who were admitted to palliative care and died between June 2016 and December 2020 and reported nausea level at the start of palliative care and possibly 1–4 weeks later. The associations between patient characteristics and nausea at the start of palliative care and change in nausea during palliative care, respectively, were studied using multiple regression analyses.
Results
Nausea level was reported at the start of palliative care by 23,751 patients of whom 8,037 also reported 1–4 weeks later. Higher nausea levels were found for women, patients with stomach or ovarian cancer, and inpatients at the start of palliative care. In multivariate analyses, cancer site was the variable most strongly associated with nausea change; the smallest nausea reductions were seen for myelomatosis and no reduction was seen for stomach cancer.
Conclusion
This study identified subgroups with the highest initial nausea level and those with the least nausea reduction after 1–4 weeks of palliative care. These latter findings should be considered in the initial treatment plan.
Insights into multiple enzymes produced by an indigenously isolated novel Aeromonas veronii strain revealing diverse applications
Authors: Yadav Dinesh , Tanveer Aiman , Gupta Supriya , Dwivedi Shruti , Yadav Kanchan , Morya Vivek 
Published: April 2022

Abstract
In the quest for a novel microbe capable of producing multiple enzymes significant in industrial applications, bacterial isolates from soil samples collected from the wood-decaying area were screened. With this aim, the isolates were screened for the capability of producing various enzymes of industrial importance. Among the 100 screened isolates of bacteria, one strain identified as Aeromonas veronii by 16s RNA sequencing was found to be promising. It was revealed that the bacteria had the ability to produce cellulase, pectinase, protease, and amylases enzymes. The strain was set up for enzyme production and enzyme activity of cellulase, amylase, pectinase and protease were 0.071, 0.201, 0.202, 0.152 U/ml respectively. All four enzymes exhibited maximum activity at 40ºC with a distinct optimal pH. Since all the enzymes isolated from this strain are industrially significant, the application of the cell-free extract containing all these enzymes was evaluated at a laboratory scale that can be applied in industrial applications. The enzyme cocktail was found to be efficient in retting Crotalaria juncea stem fibers. The enzyme in combination with the detergent was effective in removing the blood egg yolk stain and show better extraction of fruit juices and oil. We have also reported that the enzyme extract could be used for sustainable waste management.
Smart Blood Bank Management Based on Gps
Authors: Swetha K. , Tumuluru Praveen , Roshini A. 
Published: April 2022

Abstract
Blood transfusions save lives and enhance health, yet millions of patients who require transfusions are unable to obtain safe blood in a timely manner. Many individuals die as a result of the lack of safe blood, even in some urban health-care institutions. Every year, more than 81 million units of blood are collected around the world. Only 45% of them are donated in poor and transitional nations, which account for more than 80% of the global population. The main goal is to use the concept of Location-based Services to create a Geolocation based blood donor application system that allows users to find donors based on their location and improve search efficiency by using GPS and nearest neighbour algorithms, as well as to display a list of donors in the specified location.
Impact of antibiotic use before definitive concurrent chemoradiation in patients with locally advanced non-small cell lung cancer
Authors: Mei Ting , Yang Xuexi , Yu Min , Tian Xiaoman , Deng Qianyue , Chen Xianyan , Gong Youling 
Published: April 2022

Abstract
Purpose: The study evaluated whether antibiotic treatment before chemoradiotherapy influenced outcomes in patients with locally advanced non-small cell lung cancer (LA-NSCLC).Methods: The records of LA-NSCLC patients treated with chemoradiotherapy between 2010 and 2017 at xxx were retrospectively examined together with their antibiotic use (antibiotic type, duration of treatment, and time between discontinuation and chemoradiotherapy). The influence of antibiotics on progression-free survival (PFS) and overall survival (OS) was evaluated with Kaplan-Meier curves and univariate and multivariate Cox regression.Results: Of 522 patients, 176 had received intravenous broad-spectrum antibiotics in the month before chemoradiotherapy. Antibiotic use was linked to both reduced PFS (7.9 vs. 13.4 mo, p<0.001) and OS (20.4 vs. 25.3 mo, p=0.049). Multivariate regression demonstrated that antibiotic treatment was an unfavorable independent prognostic factor for LA-NSCLC patients that received chemoradiotherapy (HR, 1.234; 95% CI, 1.019-1.494; p=0.031). Prognosis was also influenced by the antibiotic type, length of treatment, and interval between discontinuation and start of chemoradiotherapy initiation. β-lactamase inhibitors were found to be the most harmful (median OS for β-lactamase inhibitors /Fluoroquinolones / Cephalosporins:16.5/19.9/25.9 mo, p=0.045). Cutoff values for interval and duration calculated by the X-tile procedure showed that intervals of 7-16 days or durations ≤6 days did not significantly affect OS relative to untreated patients (intervals: p=0.9, duration: p=0.93). Conclusions: Antibiotic treatment for longer than six days, especially with β-lactamase inhibitors, was associated with poor prognosis. Furthermore, delaying chemoradiotherapy for 7-16 days after antibiotic discontinuation may reduce these negative effects.
Does BMI modify the association between vitamin D and pulmonary function in child mild-asthma phenotype?
Authors: Papamichael Maria Michelle , Itsiopoulos Catherine , Katsardis Charis , Tsoukalas Dimitris , Erbas Bircan 
Published: April 2022

Abstract
Purpose: To investigate whether BMI modifies pulmonary function across vitamin D tertiles in pediatric asthma patients of the mild-asthma phenotype. Methods: This cross-sectional study compared lung function variability as assessed by spirometry among 35 normo-weight and 26 overweight/obese Greek schoolchildren with mild-asthma attending an urban children’s asthma clinic. Nitric oxide associated with airway inflammation was measured from exhaled breath (FeNO). Serum 25(OH)D levels ≥ 30 ng/mL were defined as ‘sufficient’, 20-30 ng/mL ‘insufficient’ and < 20 ng/mL ‘deficient’. Linear regression was used to investigate associations among vitamin D, pulmonary function tests, and BMI category. Results: Almost all children were D-insufficient (< 30 ng/mL) which did not differ by BMI status. Stratification by BMI category showed positive associations between D (continuous), % FVC (β = 0.49, 95%CI: 0.05, 0.94; Padj = 0.031) and % FEV1 (β = 0.48, 95%CI: -0.01, 0.95; Padj = 0.047) in the normo-weight only, adjusted for age, sex, regular exercise and medication. Furthermore, FEV1 was 10% higher in the normo-weight D-sufficient group compared to those D-deficient (β = 10.43, 95%CI: 0.54, 20.32; Padj = 0.040). No associations were observed for the overweight/obese group or FeNO. Conclusion: BMI modified associations of vitamin D on airway mechanics in children of the mild-asthma phenotype. Serum 25(OH)D concentrations ≥ 30 ng/mL were associated with higher ventilation in central airways of normo-weight asthmatic children.
The resonance assignments of ORC2-WH domain of human ORC protein
Authors: Song Lanlan , Tu Xiaoming , Zhang Jiahai 
Published: April 2022

Abstract
ORC2 is a small subunit of the origin recognition complex (ORC), which is important for gene replication. ORC2-WH domain recognizes dsDNA sequences with its flexible β-sheet hairpins as anchors. Here, we report the near-complete NMR backbone and side chain resonance assignments of the WH domain and study the backbone relaxation of WH domain. These studies will contribute further understanding of the relationship between its structure and function of ORC protein.
Taurine promotes differentiation and maturation of neural stem/progenitor cells from the subventricular zone via activation of GABAA receptors
Authors: Gutiérrez-Castañeda Nadia Estefanía , González-Corona Jessica , Griego Ernesto , Galván Emilio J. , Paz Lenin D. Ochoa-de la 
Published: April 2022

Abstract
Neurogenesis, the formation of new neurons in the brain, occurs throughout the lifespan in the subgranular zone of the dentate gyrus and subventricular zone (SVZ) lining the lateral ventricles of the mammal brain. In this process, gamma-aminobutyric acid (GABA) and its ionotropic receptor, the GABAA receptor (GABAAR), play a critical role in the proliferation, differentiation, and migration process of neural stem/progenitor cells (NPC). Taurine, a non-essential amino acid widely distributed throughout the central nervous system, increases the proliferation of SVZ progenitor cells by a mechanism that may involve GABAAR activation. Therefore, we characterized the effects of taurine on the differentiation process of NPC expressing GABAAR. Preincubation of NPC-SVZ with taurine increased the expression of SOX-2 and microtubule-stabilizing proteins assessed with the doublecortin assay. Taurine, like GABA, stimulated a neuronal-like morphology of NPC-SVZ and increased the number and length of primary, secondary, and tertiary neurites compared with control NPC of the SVZ. Furthermore, neurite outgrowth was prevented when simultaneously incubating cells with taurine or GABA and the GABAAR blocker, picrotoxin. Patch-clamp recordings revealed a series of modifications in the NPCs’ passive and active electrophysiological properties exposed to taurine, including regenerative spikes with kinetic properties similar to the action potentials of functional neurons.
ECMO cannulation in a non-intubated child
Authors: Weber Greta , Schock Stephanie , Fox Kenneth A. , Seckeler Michael D. , Mendelson Jenny , Kowalek Katie 
Published: April 2022

Abstract
We describe the first reported case of a child being cannulated for VA ECMO without intubation and only moderate sedation. The patient had acute fulminant cardiac failure of unknown etiology and was deemed too ill to undergo deeper sedation for intubation.
Alternating inspiration and expiration breath-hold techniques spare the chest wall during stereotactic body radiotherapy for peripheral lung malignancies
Authors: Li Wenxiang , Zhu Xinli , Bu Luyi , Zhao Feng , Xu Jiayi , Yao Guorong , Lu Zhongjie , Yan Senxiang 
Published: April 2022

Abstract
BackgroundStereotactic body radiotherapy (SBRT) for peripheral lung cancer and metastasis increases the chest wall toxicity due to its proximity to the high dose region of the PTV. Herein, we propose a solution of alternating breath-hold on half of the treatment course to spare the chest wall without compromising the PTV coverage.MethodsTwenty patients with lung lesions adjacent to the chest wall were enrolled in this study and received respiratory coaching one week in advance. Simulation CT scanning was carried out under both end-expiration breath-hold (EEBH) and deep inspiration breath-hold (DIBH), and coplanar volumetric modulated arc therapy (VMAT) plans were created on both of the two series of breath-hold CT images. Dosimetric parameters of the chest wall (V30, V40, Dmax, D0.1cc, D1cc, and D5cc) and bilateral lungs (V5, V10, V20, and MLD) were compared among plans under EEBH alone, DIBH alone, and EEBH plus DIBH. Meanwhile, the correlation between tumor location and the outcome of chest wall sparing was quantitatively evaluated. Paired t test was applied and differences with P < 0.05 were considered statistically significant.ResultsCompared to EEBH and DIBH plans, alternating breath-hold techniques result in reduction rates of 47.7% and 54.5% (V30), 56.7% and 65.4% (V40), 2.0% and 3.9% (Dmax), 6.2% and 7.6% (D0.1cc), 16.0% and 15.2% (D1cc), 14.6% and 19.1% (D5cc), respectively. Slight increase in lung V5 (< 6%), and no significant differences can be observed in lung V10, V20, and MLD. Tumor displacement with combined breath-hold appears to be greater in the lower lobes (SI/d: 1.17) than in the upper and middle lobe (SI/d: 0.18), allowing for better chest wall sparing nearly 100% in high dose volumes and 40% in high point doses.ConclusionsAlternating deep inspiration and end-expiration breath-hold techniques can significantly spare the immediately adjacent chest wall without compromising PTV coverage in SBRT of peripheral lung malignancies. Furthermore, patients with tumors in the lower lobes can obtain the most beneficial dose reduction than in the upper and middle lobes.
Determination of ketorolac tromethamine in human plasma by RP-HPLC and its application in pharmacokinetics
Authors: Zhang Chunlei , Meng xing , Liu Juan , Yu Qiang , Cheng Guoliang , Zhang Jiaqiang 
Published: April 2022

Abstract
Objective: To investigate the pharmacokinetic parameters of ketorolac tromethamine in patients after thyroid surgery. Methods: 200 human plasma samples were extracted by liquid-liquid extractionμ50. Agilent tc-c18 reversed-phase column (150mm)×4.6mm，5μm) As a chromatographic column, the mobile phase was 30mmol / L ammonium acetate acetonitrile methanol (20:15:65 V / V / V), the flow rate was 0.8ml/min, the detection column temperature was 35 ℃, the detection wavelength was 285nm, and ethyl acetate and dichloromethane (80:20 V / V) were used as extractants to detect the concentration and pharmacokinetics of aminobutanol in plasma samples by RP-HPLC. Results: ketorolac tromethamine was in the range of 10.0 ~ 240μWithin the concentration range of g/L, the peak area ratio has a good linear relationship with the concentration, and the minimum detection limit is 0.20μG / L, the relative average recovery of ketorolac tromethamine concentration at low, medium and high concentrations is greater than 97%, the extraction recovery is greater than 80%, and the intraday and inter-day variation are less than 10%. The linear equation is y = 8.63x + 1.27, r = 0.99899 (n = 30).There was no significant difference in the drug time curve between intravenous drip and intravenous pump Administration (P = 0.851), and there was a significant difference between intravenous drip and intramuscular injection. At the same time, there was no significant difference in pharmacokinetic parameters between intravenous drip and intramuscular injection in half-life, peak time, and apparent distribution volume. The peak concentration of intravenous drip was higher than that of intramuscular injection,24-hour AUC intravenous infusion was higher than intramuscular injection. Conclusion: based on the RP-HPLC method for the determination of ketorolac tromethamine concentration in human plasma, it has the advantages of simple operation, high sensitivity, good compliance, and strong specificity. It is accurate and reliable for clinical results. Based on the above detection methods, it is found that there is no difference in the drug-time curves of intravenous drip and intravenous pump, which are different from that of intramuscular injection, At the same time, the pharmacokinetic parameters of intravenous drip seem to be better than intramuscular injection. Trial registration: ChiCTR, ChiCTR2000030674. Registered 9 March 2020, http://www.chictr.org.cn/
The clock gene Period1 inhibits the mineralization of cementoblast through the Wnt/β-catenin pathway
Authors: Zhu Guixin , Zhang Chao , Liu Gufeng , Lu Qian , Xia Dahong , Cao Zhengguo , Chen Zhijun , Xia Haibin 
Published: April 2022

Abstract
The biological clock regulated by circadian clock genes manipulates various tooth tissues including enamel, dentin, and periodontal ligament. However, whether these genes regulate cementum regeneration remains unclear. This study aims to explore whether Per1, a core gene of circadian clock genes, affects the mineralization of cementum. Immunohistochemical staining and hematoxylin-eosin staining were performed on 6-weeks-old C57BL/6 mice and showed that there were PER1-positive cells in the cementum. In addition, the expression of Per1 decreased during the mineralization of the murine cementoblast cell line OCCM-30. These findings suggest that Per1 may regulate cementum mineralization. To verify the effect of Per1 on cementum mineralization, Per1 was knocked down by lentiviral transduction in OCCM-30. The results showed that knocking down Per1 promoted the mineralization of OCCM-30, whilst it up-regulated the Wnt/β-catenin pathway. Taken together, Per1 can inhibit cementum mineralization via the Wnt/β-catenin pathway, providing a molecular foundation for the treatment of cementum loss caused by periodontal diseases.
Structural reliability of existing RC beams strengthened with UHPFRC tensile layers
Authors: Simwanda Lenganji , De Koker Nico , Viljoen Celeste , Bababfemi Adewumi John 
Published: April 2022

Abstract
A methodology for reliability analysis of reinforced (RC) beams strengthened with ultra high-performance fibre reinforced concrete (UHPFRC) tensile layers is presented. The proposed methodology includes stochastic stress-block analysis of a section, assuming a perfect bond between the RC beam and the UHPFRC layer. Annual reliability analysis of the RC beam before and after the strengthening operation is conducted. Deterioration induced by chloride corrosion is incorporated into the analysis via a chloride induced corrosion model based on Fick’s law of diffusion and described stochastically to account for the epistemic uncertainty in the time to corrosion initiation and rate of corrosion. A plot for determining the required thickness of the UHPFRC tensile layer to upgrade to the required reliability level is also given, considering the time from construction when the strengthening operation is conducted. The proposed approach is easy to apply for routine practice.
Comparison of visual outcomes, keratometric parameters and biomechanical profiles between deep anterior lamellar keratoplasty with big-bubble technique vs. Melles technique for keratoconus: A retrospective study
Authors: Jamali Hossein , Saluoti Ramin , Maalhagh Mehrnoosh , Hosseini Shahla , Shirvani Mohammad 
Published: April 2022

Abstract
Background: Advances in surgical techniques introduced deep anterior lamellar keratoplasty (DALK) as an option for patients with advanced keratoconus treatment. This method saves globe integrity and preserves the endothelial cells. In this study, two DALK techniques are compared in different aspects.Methods: In This retrospective comparative clinical study, thirty-seven eyes treated by big bubble DALK technique and 35 eyes with Melles technique. The outcome measures consist of uncorrected visual acuity (UCVA), best corrected spectacle visual acuity (BSCVA), manifest refraction, keratometric features, contrast sensitivity, corneal aberrations, corneal biomechanical properties, endothelial cells profile.Results: Mean UCVA in the big bubble group was 0.61±25 LogMAR and in the Melles group was 0.89±0.41 LogMAR (p-value 0.043). Mean BSCVA in big bubble group (0.18±0.12 Log MAR) was significantly better than Melles group (0.35±0.16 Log MAR). Mean of sphere and cylinder refraction showed no significant difference between two groups. Comparison of endothelial cell profile, corneal aberrations, corneal biomechanical properties and keratometry had no remarkable differences. Contrast sensitivity reported as Modulation transfer function (MTF) showed higher values in big bubble group and differences with Melles group were remarkable. Results of point spread function (PSF) in big bubble group had superiority to Melles group with considerable statistical P value of 0.023.Conclusions: Big bubble technique creates smooth interface with minimal stromal residue that leads in better visual quality and contrast sensitivity compared with Melles technique.