bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recent advances of transcriptomics and proteomics in triple‐negative breast cancer prognosis assessment
Authors: Li Yuan , Kong Xiangyi , Wang Zhongzhao , Xuan Lixue 
Published: February 2022

AbstractTriple‐negative breast cancer (TNBC), a heterogeneous tumour that lacks the expression of oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), is often characterized by aggressiveness and tends to recur or metastasize. TNBC lacks therapeutic targets compared with other subtypes and is not sensitive to endocrine therapy or targeted therapy except chemotherapy. Therefore, identifying the prognostic characteristics and valid therapeutic targets of TNBC could facilitate early personalized treatment. Due to the rapid development of various technologies, researchers are increasingly focusing on integrating ‘big data’ and biological systems, which is referred to as ‘omics’, as a means of resolving it. Transcriptomics and proteomics analyses play an essential role in exploring prospective biomarkers and potential therapeutic targets for triple‐negative breast cancers, which provides a powerful engine for TNBC’s therapeutic discovery when combined with complementary information. Here, we review the recent progress of TNBC research in transcriptomics and proteomics to identify possible therapeutic goals and improve the survival of patients with triple‐negative breast cancer. Also, researchers may benefit from this article to catalyse further analysis and investigation to decipher the global picture of TNBC cancer.
Rapid detection of florfenicol antibiotic residues in chicken meat using surface‐enhanced Raman spectroscopy
Authors: Behvarmanesh Mehran , Malekfar Rasoul , Asadi Gholamhassan , Etezad Seyed Masoud 
Published: February 2022

AbstractIn this study, the surface‐enhanced Raman spectroscopy (SERS) method was applied to determine florfenicol residues (FF) in chicken meat. Gold nanoparticles were stabilized on the glass slides cost‐effectively by “1‐dodecanethiol.” Three factors, including “solvent types,” “spectroscopy times,” and “ ratio of gold nanoparticles and samples” were examined to optimize the spectrum acquisition. The theoretical Raman spectrum of FF was calculated to compare the experimental spectrum (Asymp. Sig. = 0.0655). FF fingerprint was determined in solid and water‐soluble states, and the index peak assignment was performed. In the quantitative measurements of FF, a value of LOD = 0.25 μg/L was obtained, and its linear regression equation was calculated. In the field activities, FF was added to chickens drinking water (including five dosages in three replications), and FF residues were determined by the SERS and LC/MS/MS methods. The results showed no statistically meaningful differences (Asymp. Sig. = 0.714, p < .01).
Oxytocin as a potential pharmacological tool to combat obesity
Authors: Olszewski Pawel K. , Noble Emily E. , Paiva Luis , Ueta Yoichi , Blevins James E. 
Published: February 2022

AbstractThe neuropeptide oxytocin (OT) has emerged as an important anorexigen in the regulation of food intake and energy balance. It has been shown that the release of OT and activation of hypothalamic OT neurons coincide with food ingestion. Its effects on feeding have largely been attributed to limiting meal size through interactions in key regulatory brain regions governing the homeostatic control of food intake such as the hypothalamus and hindbrain in addition to key feeding reward areas such as the nucleus accumbens and ventral tegmental area. Furthermore, the magnitude of an anorexigenic response to OT and feeding‐related activation of the brain OT circuit are modified by the composition and flavor of a diet, as well as by a social context in which a meal is consumed. OT is particularly effective in reducing consumption of carbohydrates and sweet tastants. Pharmacologic, genetic, and pair‐feeding studies indicate that OT‐elicited weight loss cannot be fully explained by reductions of food intake and that the overall impact of OT on energy balance is also partly a result of OT‐elicited changes in lipolysis, energy expenditure, and glucose regulation. Peripheral administration of OT mimics many of its effects when it is given into the central nervous system, raising the questions of whether and to what extent circulating OT acts through peripheral OT receptors to regulate energy balance. Although OT has been found to elicit weight loss in female mice, recent studies have indicated that sex and estrous cycle may impact oxytocinergic modulation of food intake. Despite the overall promising basic research data, attempts to use OT in the clinical setting to combat obesity and overeating have generated somewhat mixed results. The focus of this mini‐review is to briefly summarize the role of OT in feeding and metabolism, address gaps and inconsistencies in our knowledge, and discuss some of the limitations to the potential use of chronic OT that should help guide future research on OT as a tailor‐made anti‐obesity therapeutic.
The correlation of systemic immune‐inflammation index, neutrophil‐to‐lymphocyte ratio, derived neutrophil‐to‐lymphocyte ratio, and platelet‐to‐lymphocyte ratio with disease severity in recurrent aphthous stomatitis
Authors: Tanacan Efsun , Dinçer Rota Didem , Oktem Rumeysa , Erdogan Fatma Gulru 
Published: February 2022

AbstractBackground/AimTo evaluate the correlation of systemic immune inflammation index (SII), neutrophil‐to‐lymphocyte ratio (NLR), derived neutrophil‐to‐lymphocyte ratio (NLR), and platelet‐to‐lymphocyte ratio (PLR) with disease severity in recurrent aphthous stomatitis (RAS).MethodsThe present retrospective cohort study was performed on patients with RAS. Patients were divided into three groups: 1) Major (n = 75), 2) Minor (n = 123), and 3) Herpetiform aphthae (n = 17). The study groups were compared in terms of demographic features, acute phase proteins, and complete blood cell count parameters. Moreover, correlation analyses were performed for the correlation of ulcer severity score (USS) with C‐reactive protein (CRP), SII, erythrocyte sedimentation rate (ESR), ferritin, dNLR, NLR, and PLR.ResultsSignificantly higher values were observed for USS, neutrophil count, ferritin, SII, NLR, and dNLR (p < 0.05 for all) in the major and herpetiform aphthae groups compared to the minor aphthae group. Positive, strong, significant correlations were observed between USS, SII, and NLR (r = 0.80 for SII, r = 0.74 for NLR and p < 0.001 for both). Positive, moderate, significant correlations were observed between USS, PLR, ESR, and CRP (r = 0.54 for PLR, r = 0.39 for ESR, r = 0.36 for CRP, and p < 0.001 for all). Positive, weak, significant correlations were observed between USS, dNLR, and ferritin (r = 0.13 and p = 0.05 for ferritin, r = 0.27 and p < 0.001 for dNLR).ConclusionHigher values of SII, NLR, dNLR, and PLR were associated with disease severity in patients with RAS.
Evaluation of five collagenase clostridium histolyticum‐aaes injection techniques for the treatment of cellulite on the buttock or thigh
Authors: Kaufman‐Janette Joely , Katz Bruce E. , Vijayan Saji , Xiang Qinfang , Kaminer Michael S. 
Published: February 2022

AbstractBackgroundGiven differences in buttock versus thigh cellulite, collagenase clostridium histolyticum‐aaes (CCH‐aaes) injection technique may impact treatment effects at these sites.AimTo evaluate efficacy and safety of 5 CCH‐aaes injection techniques.MethodsA phase 2A, open‐label trial enrolled women with mild‐to‐severe cellulite (Clinician Reported Photonumeric Cellulite Severity Scale) on both buttocks or thighs. CCH‐aaes 0.84 mg was administered as 12 injections in each of two buttock or two thigh treatment areas (total dose, 1.68 mg) during three treatment sessions (Days 1, 22, 43). On Day 1, women were sequentially assigned to: Technique A = shallow injection/3 aliquots; Technique B = shallow injection/1 aliquot; Technique C = deep injection/1 aliquot; Technique D = deep and shallow injections/5 aliquots; or Technique E = shallow injection/4 aliquots. Change from baseline in Hexsel Cellulite Severity Scale (CSS) depression depth (range, 0 [no depressions] to 3 [deep depressions]) was assessed at Day 71. Safety was evaluated via adverse events.ResultsSixty‐three women with buttock (n = 31) or thigh (n = 32) cellulite received ≥1 CCH‐aaes dose. For buttock cellulite, CCH‐aaes injection Technique A resulted in the greatest baseline‐adjusted improvement in CSS score on Day 71 (least‐squares mean, 1.17‐point improvement). For thigh cellulite, CSS score improvement was greatest with Technique D (least‐squares mean, 1.40‐point improvement). CCH injection Techniques A, D, and E were associated with more favorable safety profiles than Techniques B and C.ConclusionDifferent CCH‐aaes injection techniques are required with buttock (Technique A) versus thigh (Technique D) cellulite to optimize treatment outcomes.
Volatility Expectations and Returns
Authors: LOCHSTOER LARS A. , MUIR TYLER 
Published: February 2022

ABSTRACTWe provide evidence that agents have slow‐moving beliefs about stock market volatility that lead to initial underreaction to volatility shocks followed by delayed overreaction. These dynamics are mirrored in the VIX and variance risk premiums, which reflect investor expectations about volatility, and are also supported in both surveys and firm‐level option prices. We embed these expectations into an asset pricing model and find that the model can account for a number of stylized facts about market returns and return volatility that are difficult to reconcile, including a weak or even negative risk‐return trade‐off.
Effect of Denture Hygiene Protocols on Patient Satisfaction, Oral Health‐Related Quality of Life, and Salivary Parameters: A Randomized Clinical Trial
Authors: Barbosa Ribeiro Adriana , Borba Araújo Camila , Vieira Fortes Caroline , Lucarini Bueno Frank , de Cássia Oliveira Viviane , Macedo Ana Paula , de Freitas Oliveira Paranhos Helena , Watanabe Evandro , De Wever Bart , Helena Silva‐Lovato Cláudia 
Published: February 2022

AbstractPurposeThis randomized controlled trial compared four denture hygiene protocols in terms of patient satisfaction, oral health‐related quality of life (OHRQoL), and salivary parameters in complete denture wearers with denture stomatitis.Material and methodsFor this randomized, double‐blind, controlled clinical trial, 108 participants were assigned to soak their dentures in one of the following solutions: (1) 0.25% sodium hypochlorite (positive control), (2) 0.15% Triclosan, (3) denture disinfecting tablets, or (4) denture disinfecting tablets plus palatine mucosa brushing solution. The outcomes of patient satisfaction, OHRQoL, and salivary parameters (salivary flow rate and pH) were measured at baseline and after 10 days. The Kruskal‐Wallis and Dunn tests (between groups), and Wilcoxon test (between times) were used to compare the results. (α = 0.05).ResultsAfter the hygiene protocols, and when compared with baseline, the overall patient satisfaction, maxillary denture satisfaction, maxillary denture comfort, and maxillary denture retention were ameliorated. A significant improvement was noted in OHRQoL in 3 of 4 domains evaluated (orofacial pain and discomfort, masticatory discomfort and disability, and psychological disability and discomfort). The salivary flow rate (unstimulated and stimulated) and salivary pH were not significantly affected at the times evaluated.ConclusionsComplete denture wearers may feel more satisfied with their complete dentures when treated for denture stomatitis. The tested treatments lead to similar improvement in terms of patient satisfaction and OHRQoL.
Comparison of Crestal Bone Loss and Osteocalcin Release Kinetics in Immediately and Delayed Loaded Implants: A Randomized Controlled Trial
Authors: Keshari Rishabh , Chand Pooran , Singh Balendra P. , Jurel Sunit K. , Singh Ranjana , Singh Punit Kumar , Solanki Neeti 
Published: February 2022

AbstractPurposeTo compare concentration and release kinetics of osteocalcin and crestal bone loss under immediate and delayed loading conditions during osseointegration.Materials and methodsForty‐one patients who were indicated for rehabilitation with dental implants randomly received either implant with placement of permanent prosthesis after 3 months (delayed loading) or implant with placement of permanent prosthesis within 7 days (immediate loading). Radiographic assessment of crestal bone loss at the mesial and distal surface was done at 3, 6, and 12 months after implant placement. Peri‐implant sulcular fluid was collected immediately from the buccal surface at two sites after implant insertion and also, at 7, 15, 30, and 90 days after surgery. The level of osteocalcin was evaluated using ELISA and data were compared using two sample t‐test. Differences between two groups were analyzed by unpaired Student's t test. Intragroup comparison was done by repeated measures ANOVA.ResultsMean crestal bone loss was lower in the immediate loading group compared to the delayed loading group at 3, 6, and 12 months (p < 0.001). Intragroup comparison revealed a statistically significant increase in osteocalcin levels in both group I (delayed loading) (F = 26712.2) and group II (immediate loading) (F = 10497.2) at the predetermined time intervals.ConclusionsLess crestal bone loss and early release of osteocalcin was found in the immediately loaded group than in the delayed loaded group. The study substantiates that immediately loaded implants show less crestal bone as well as early release of osteocalcin facilitating upregulation of bone metabolism, improving long term health of bone and prognosis of implants. Immediately loaded implants can be a better treatment protocol provided there is adequate bone and primary stability.
Treatment of primary hyperparathyroidism in a Miniature Horse using chemical ablation of abnormal parathyroid tissue localized by 3‐phase computed tomography
Authors: Colmer Sarah F. , Wulster Kathryn , Johnson Amy L. , Levine David G. , Underwood Claire , Watkins Trevor W. , Van Eps Andrew W. 
Published: February 2022

AbstractA 15‐year‐old Miniature Horse mare with persistently increased plasma calcium (total and ionized) and serum parathyroid hormone concentrations was presented for suspected primary hyperparathyroidism. Ultrasonography of the thyroid region identified an enlarged heterogeneous mass axial to the right thyroid lobe suggestive of an enlarged parathyroid gland, which was further confirmed using sestamibi nuclear scintigraphy and 3‐phase computed tomography. Percutaneous ultrasound‐guided ethanol ablation of the mass, a method not previously described in the horse, was performed under general anesthesia resulting in rapid normalization of plasma ionized calcium and serum parathyroid hormone concentrations. Ablation of abnormal parathyroid gland tissue may be a suitable alternative to surgical resection in certain cases of primary hyperparathyroidism in the horse.
Telomere length is highly repeatable and shorter in individuals with more elaborate sexual ornamentation in a short‐lived passerine
Authors: Kauzálová Tereza , Tomášek Oldřich , Mulder Ellis , Verhulst Simon , Albrecht Tomáš 
Published: February 2022

AbstractQuantifying an individual's state as a fitness proxy has proven challenging, but accumulating evidence suggests that telomere length and attrition may indicate individual somatic state and success at self‐maintenance, respectively. Sexual ornamentation is also thought to signal phenotypic quality, but links between telomeres and sexual ornamentation have been little explored. To address this issue, we examined whether telomere length and dynamics are predicted by the expression of a sexually selected ornament, the length of the outermost tail feathers (streamers), using longitudinal data from a population of European barn swallows (Hirundo rustica). In 139 adult individuals, each measured twice, we further assessed associations of telomere length with age, sex, breeding status and survival. Telomere length showed high individual repeatability (R = .97) across years while shortening with age in both sexes. Telomere length and dynamics were not significantly associated with survival to the next year, remaining lifespan or reproduction status (comparing breeding and nonbreeding yearlings). Tail streamer length, a sexually selected trait in barn swallows, was negatively associated with telomere length, independent of sex. Thus, telomere length may reflect the costs of carrying an elaborated sexual ornament, although ornament size did not significantly predict telomere shortening. In conclusion, telomere length in adult barn swallows is a highly consistent trait that shows a negative relationship with sexual ornamentation, suggesting a trade‐off between sexual ornamentation and telomere length.
Bertrand–Edgeworth oligopoly: Characterization of mixed strategy equilibria when some firms are large and the others are small
Authors: De Francesco Massimo A. , Salvadori Neri 
Published: February 2022

AbstractThis paper studies Bertrand–Edgeworth competition among firms producing a homogeneous commodity under efficient rationing and constant (and identical across firms) marginal cost until full capacity utilization is reached. Our focus is on a subset of the no pure‐strategy equilibrium region of the capacity space in which, in a well‐defined sense, some firms are large and the others are small. We characterize equilibria for such subset. For each firm, the payoffs are the same at any equilibrium and, for each type of firm, they are proportional to capacity. While there is a single profile of equilibrium distributions for the large firms, there is a continuum of equilibrium distributions for the small firms: what is uniquely determined, for the latter, is the capacity‐weighted sum of their equilibrium distributions and hence the union of the supports of their equilibrium strategies.
Histological diagnosis from kidney transplant biopsy can contribute to prediction of graft survival
Authors: Nasic Salmir , Mölne Johan , Stegmayr Bernd , Peters Björn 
Published: February 2022

AbstractAimThe primary aim of this study was to in depth examine if the histological findings in a transplanted kidney biopsy can predict the prognosis for the graft and the patient. The secondary aim was to extend knowledge of the impact of time elapsed on biopsy findings.MethodsData from 1462 patients were merged from a kidney transplantation registry and a biopsy registry during 1 January 2007 and 30 September 2017. Kaplan–Meier analysis and multivariate Cox‐regression analysis were performed and hazard ratios (HR) with 95% confidence intervals (CI) were presented.ResultsCompared to normal biopsy findings, graft survival after biopsy (gsaBiopsy) was shorter for patients with glomerular diseases (HR 8.2, CI:3.2–21.1), rejections (HR 4.2, CI:1.7–10.3), chronic changes including IFTA (HR 3.2, CI:1.3–8.0), acute tubular injuries (HR 3.0, CI:1.2–7.8), and borderline changes (HR 2.9, CI:1.1–7.6). Sub‐analysis of rejections showed shorter gsaBiopsy for chronic TCMR (HR 4.7, CI:1.9–11.3), active ABMR (HR 3.6, CI:1.7–7.7) and chronic ABMR (HR 3.5, CI:2.0–6.0). Patients with TCMR Banff grade II (HR 0.35, CI:0.20–0.63) and grade I (HR 0.52, CI:0.29–0.93) had a better gsaBiopsy compared to all other types of rejections.ConclusionShorter gsaBiopsy was noted in kidneys with glomerular diseases, rejections, acute tubular injuries and borderline changes. TCMR Banff rejections grade I and II were associated with a better prognosis.
Tyrosine sulphation of CXCR4 induces the migration of fibroblast in OSF
Authors: He Caiyun , Wang Weiming , Wan Wendong , Liang Jie , Hu Jiaju , Yuan Yongxiang , Jiang Canhua , Li Ning 
Published: February 2022

AbstractOral submucous fibrosis (OSF) caused by areca nut chewing is a prevalent fibrotic disease in Asia‐Pacific countries. Arecoline‐induced migration of fibroblasts (FBs) plays a vital role in the development of OSF. However, the specific molecular mechanisms involved remain unclear. Many studies have shown that tyrosine sulphation of chemokines can influence cell migration. Herein, we demonstrated that arecoline stimulates tyrosine sulphation of the chemokine receptor 4 (CXCR4) through the tyrosylprotein sulphotransferase‐1 (TPST‐1) to enhance the migration ability of FBs. Moreover, by RNA‐Seq analysis, we found that the most significantly altered pathway was the EGFR pathway after the arecoline stimulation for FBs. After the knockdown of arecoline‐induced EGFR expression, the tyrosine sulphation of CXCR4 was significantly decreased by the inhibition of TPST‐1 induction. Finally, in human OSF specimens, TPST‐1 expression was directly correlated with the expression of CXCR4. These data indicate that the arecoline‐induced tyrosine sulphation of CXCR4, which is regulated by TPST‐1, might be a potential mechanism that contributes to FB migration in OSF.
CTHRC1 expressed in periodontitis and human periodontal fibroblasts exposed to inflammatory stimuli
Authors: Huang Xue‐Ying , Guan Wei‐Qun 
Published: February 2022

AbstractObjectivesCollagen triple helix repeat containing‐1 (CTHRC1) is a glycoprotein that can be secreted extracellularly and is involved in the regulation of collagen matrix in a variety of diseases. The expression level of CTHRC1 in periodontitis was detected in this study.Materials and MethodsThe gingival tissues from clinically healthy subjects (15 cases) and those with periodontitis (30 cases) were taken for immunohistochemical staining. Lipopolysaccharide of the Porphyromonas gingivalis was added in the periodontal ligament fibroblast culture in vitro. Cells were collected, and the mRNA levels of the intracellular CTHRC1 and protein expression of the extracellular CTHRC1 were detected.ResultsThe protein expression of CTHRC1 in the periodontitis group was higher than that of the clinically healthy group. The in vitro cell experiments showed that 10 μg/ml of P.g LPS could induce a significant increase in protein secretion of CTHRC1, and 5 μg/ml P.g LPS had a significant effect on promoting the mRNA expression of CTHRC1.ConclusionsCollagen triple helix repeat containing‐1 might be involved in the development of periodontitis, and the expression level might be significantly correlated with the stimulation of P.g LPS on fibroblasts. Different stimulation intensities of P.g LPS might result in different expression patterns of CTHRC1.