bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved diagnosis of rheumatoid arthritis using an artificial neural network
Authors: Bai Linlu , Zhang Yuan , Wang Pan , Zhu Xiaojun , Xiong Jing-Wei , Cui Liyan 
Published: June 2022

AbstractRheumatoid arthritis (RA) is chronic systemic disease that can cause joint damage, disability and destructive polyarthritis. Current diagnosis of RA is based on a combination of clinical and laboratory features. However, RA diagnosis can be difficult at its disease onset on account of overlapping symptoms with other arthritis, so early recognition and diagnosis of RA permit the better management of patients. In order to improve the medical diagnosis of RA and evaluate the effects of different clinical features on RA diagnosis, we applied an artificial neural network (ANN) as the training algorithm, and used fivefold cross-validation to evaluate its performance. From each sample, we obtained data on 6 features: age, sex, rheumatoid factor, anti-citrullinated peptide antibody (CCP), 14-3-3η, and anti-carbamylated protein (CarP) antibodies. After training, this ANN model assigned each sample a probability for being either an RA patient or a non-RA patient. On the validation dataset, the F1 for all samples by this ANN model was 0.916, which was higher than the 0.906 we previously reported using an optimal threshold algorithm. Therefore, this ANN algorithm not only improved the accuracy of RA diagnosis, but also revealed that anti-CCP had the greatest effect while age and anti-CarP had a weaker on RA diagnosis.
Screening and diagnosis of hemoglobinopathies in Germany: Current state and future perspectives
Authors: Aramayo-Singelmann Carmen , Halimeh Susan , Proske Pia , Vignalingarajah Abinuja , Cario Holger , Christensen Morten O. , Yamamoto Raina , Röth Alexander , Reinhardt Dirk , Reinhardt Hans Christian , Alashkar Ferras 
Published: June 2022

AbstractThis monocentric study conducted at the Pediatric and Adult Hemoglobinopathy Outpatient Units of the University Hospital of Essen summarizes the results of hemoglobinopathies diagnosed between August 2018 and September 2021, prior to the introduction of a general newborn screening (NBS) for SCD in Germany (October 2021). In total, 339 patients (pts.), 182 pediatric [50.5% males (92/182)] and 157 adult pts. [75.8% females (119/157)] were diagnosed by molecular analysis. The most common (parental) descent among affected pts. were the Middle Eastern and North African/Turkey (Turkey: 19.8%, Syria: 11.8%, and Iraq: 5.9%), and the sub-Saharan African region (21.3%). Median age at diagnosis in pediatric carriers [N = 157; 54.1% males (85/157)] was 6.2 yrs. (range 1 (months) mos.–17.8 yrs.) and 31 yrs. (range 18–65 yrs.) in adults [N = 53; 75.2% females (115/153)]. Median age at diagnosis of homozygous or compound-heterozygous disease in pediatric pts. (72% (18/25) females) was 3.7 yrs., range 4 mos.–17 yrs. (HbSS (N = 13): 2.5 yrs., range 5 mos.–7.8 yrs.; HbS/C disease (N = 5): 8 yrs., range 1–8 yrs.; homozygous/compound heterozygous β-thalassemia (N = 5): 8 yrs., range 3–13 yrs.), in contrast to HbH disease (N = 5): 18 yrs. (median), range 12–40 yrs. Hemoglobinopathies represent a relevant health problem in Germany due to immigration and late diagnosis of second/third generation migrants. SCD-NBS will accelerate diagnosis and might result in reduction of disease-associated morbidity. However, diagnosis of carriers and/or disease-states (i.e. thalassemic syndromes) in newly immigrated and undiagnosed patients will further be delayed. A first major step has been taken, but further steps are required.
Role of CD47 in tumor immunity: a potential target for combination therapy
Authors: Huang Jing , Liu Fangkun , Li Chenglong , Liang Xisong , Li Chuntao , Liu Yuanyuan , Yi Zhenjie , Zhang Liyang , Fu Siqi , Zeng Yu 
Published: June 2022

AbstractCD47 performs a vital function in cancer therapy by binding to different SIRPα, thrombospondin 1, and integrin. However, its role in tumor immunity and its correlation with prognosis among many cancer types remain unknown. The raw mRNA expression data of CD47 in cancer patients was downloaded from TCGA and GTEx datasets. The protein expression of CD47 was detected using a microarray. Kaplan Meier analysis and forest plot were performed to compare the effects of high and low expression of CD47 on overall survival in different cancers. In addition, the correlations between CD47 expression and immune cell infiltration, stromal components, immune checkpoint genes, tumor mutational burden (TMB), and microsatellite instability (MSI) were analyzed from the public database. The gene function was determined by Gene Set Enrichment Analysis (GSEA). The expressions of CD47 in CHOL, COAD, ESCA, HNSC, KIRC, STAD, and THCA were higher compared with normal tissues. Elevated expression of CD47 predicted poor prognosis in ACC, KICH, KIRP, LGG, PAAD and UCEC. CD47 expression was strongly associated with immune infiltrating cells among KICH, KIRP, LGG, and PAAD. In addition, significant positive correlations with most immune checkpoint genes including PDCD 1 (PD-1), CD274 (PD-L1), CTLA4 in BLCA, DLBC, KICH, KIRC, LUAD, LUSC, PAAD, PCPG, SKCM, STAD, UCEC, and UVM was noted for the expression of CD47. GSEA analysis demonstrated that CD47 was a key regulator in metabolism-related pathways. These findings provide novel evidence that CD47 could be utilized as a promising prognostic biomarker and combination treatment target in various cancers.
Construction and analysis of a ceRNA network and patterns of immune infiltration in chronic rhinosinusitis with nasal polyps: based on data mining and experimental verification
Authors: Chen Jing-cai , Xing Qi-long , Yang Hui-wen , Yang Fan , Luo Yao , Kong Wei-jia , Wang Yan-jun 
Published: June 2022

AbstractRecent studies have revealed the significant role of the competing endogenous RNA (ceRNA) network in human diseases. However, systematic analysis of the ceRNA mechanism in chronic rhinosinusitis with nasal polyps (CRSwNP) is limited. In this study, we constructed a competitive endogenous RNA (ceRNA) network and identified a potential regulatory axis in CRSwNP based on bioinformatics analysis and experimental verification. We obtained lncRNA, miRNA, and mRNA expression profiles from the Gene Expression Omnibus. After analysis of CRSwNP patients and the control groups, we identified 565 DE-lncRNAs, 23 DE-miRNAs, and 1799 DE-mRNAs by the DESeq2 R package or limma R package. Enrichment analysis of 1799 DE-mRNAs showed that CRSwNP was associated with inflammation and immunity. Moreover, we identified 21 lncRNAs, 8 miRNAs and 8 mRNAs to construct the lncRNA-miRNA-mRNA ceRNA network. A potential MIAT/miR-125a/IRF4 axis was determined according to the degree and positive correlation between a lncRNA and its competitive endogenous mRNAs. The GSEA results suggested that IRF4 may be involved in immune cell infiltration. The validation of another dataset confirmed that MIAT and IRF4 were differentially expressed between the CRSwNP and control groups. The area under the ROC curve (AUC) of MIAT and IRF4 was 0.944. The CIBERSORT analysis revealed that eosinophils and M2 macrophages may be involved in the CRSwNP process. MIAT was correlated with dendritic cells and M2 macrophages, and IRF4 was correlated with dendritic cells. Finally, to validate the key genes, we performed in-silico validation using another dataset and experimental validation using immunohistochemistry, immunofluorescence, and Western blot. In summary, the constructed novel MIAT/miR-125a/IRF4 axis may play a critical role in the development and progression of CRSwNP. We believe that the ceRNA network and immune cell infiltration could offer further insight into novel molecular therapeutic targets for CRSwNP.
Digital body mapping of pain quality and distribution in athletes with longstanding groin pain
Authors: Serner Andreas , Reboul Gilles , Lichau Olivier , Weir Adam , Heijboer Willem , Vuckovic Zarko , Boudreau Shellie Ann 
Published: June 2022

AbstractGroin pain is common in athletes, but remains a challenge to diagnose. Self-reported pain quality distribution may facilitate differential diagnoses. We included 167 athletes with groin pain (≥ 4 weeks). All athletes received a standardized clinical examination. Athletes could choose multiple quality descriptors and intensity, and drew these on a digital body map. Overlay images were created to assess distribution and area visually. Intensity, duration, and qualities were compared between each clinical entity and multiple entities. Top three quality descriptors were electric (22%), pain (19%), and dull/aching (15%). There were no differences in the frequencies of quality descriptors (p = 0.893) between clinical entities. Areas of the mapped qualities were similar between the single clinical entities (χ2(3) = 0.143, p = 0.986) and independent of symptom duration (ρ = 0.004, p = 0.958). Despite a considerable overlap, the mapped pain qualities’ distributions appear to differ visually between single clinical entities and align with the defined clinical entities of adductor-related, inguinal-related, and pubic-related groin. In iliopsoas-related groin pain, pain extended more medially. The overlap between the drawn areas underscores a challenge in differentiating groin pain classifications based only on self-reported pain. The prevalence of pain quality descriptors varied and individually do not associate with one particular clinical entity of groin pain.
Interannual fluctuations in connectivity among crab populations (Liocarcinus depurator) along the Atlantic-Mediterranean transition
Authors: Ojeda Víctor , Serra Bruna , Lagares Clàudia , Rojo-Francàs Eva , Sellés Maria , Marco-Herrero Elena , García Encarnación , Farré Marc , Arenas Concepció , Abelló Pere , Mestres Francesc 
Published: June 2022

AbstractAn interesting evolutionary question that still remains open is the connectivity between marine populations. Marine currents can favour the dispersal of larvae or adults, but they can also produce eddies and gyres generating oceanographic fronts, thus limiting gene flow. To address this subject, we selected the Atlantic-Mediterranean transition, where several fronts are located: Gibraltar Strait (GS), Almeria-Oran Front (AOF) and Ibiza Channel (IC). Seven populations of the marine crab Liocarcinus depurator (Cadiz, West and East Alboran, Alacant, Valencia, Ebro Delta and North Catalonia) located along this transition were analysed in six consecutive years (2014–2019) using a fragment of the COI (Cytochrome Oxidase subunit I) gene. All sequences (966) belonged to two well defined haplogroups: ATL (most abundant in Atlantic waters) and MED (predominant in Mediterranean waters). Following a geographic variation, the frequency of ATL decreased significantly from Cadiz to North Catalonia. However, this variation presented steps due to the effect of oceanographic restrictions/fronts. Significant effects were recorded for GS (2015, 2017, 2018 and 2019), AOF (all years except 2018) and IC (2016). The intensity and precise location of these fronts changed over time. Multivariate analyses distinguished three main population groups: Cadiz, Alboran Sea and the remaining Mediterranean populations. These findings could be relevant to properly define Marine Protected Areas and for conservation and fisheries policies.
Differences in stress response between two altitudes assessed by salivary cortisol levels within circadian rhythms in long-distance runners
Authors: Tsunekawa Katsuhiko , Ushiki Kazumi , Martha Larasati , Nakazawa Asuka , Hasegawa Rika , Shimizu Risa , Shimoda Nozomi , Yoshida Akihiro , Nakajima Kiyomi , Kimura Takao , Murakami Masami 
Published: June 2022

AbstractThere are conflicting reports regarding the efficacy of cortisol as a stress marker in altitude training due to the influence of the circadian rhythm. This study aimed to verify whether the automated measurement of salivary cortisol concentration via sequential sampling could detect the differences in exercise stress between two altitudes. We enrolled 12 elite female long-distance runners living near sea level. For the first higher-altitude camp, the runners lived at 1800 m and trained at 1700 m for 7 days. For the second lower-altitude camp, they lived at 1550 m and trained at 1300 m for 7 days. Their saliva was sequentially collected on the last 2 days during each camp which involved different intensity exercises in the morning and afternoon. The salivary cortisol concentrations were measured using electrochemiluminescence immunoassay. Before dinner, the basal salivary cortisol concentrations were significantly higher in the higher-altitude camp. The rate of change in the salivary cortisol concentration during the morning exercise was significantly higher in the higher-altitude camp than in lower-altitude camp (p = 0.028) despite the same exercise programs and intensities. Salivary cortisol level measurements during the athletes' circadian rhythms could detect the differences in acclimatization and exercise stress between two altitudes.
The relationship between ocular and oral dryness in a cohort from the 65-year-old population in Norway
Authors: Hynne Håvard , Tashbayev Behzod , Diep My Tien , Sødal Anne Thea Tveit , Badian Reza A. , Chen Xiangjun , Lai Xiaoran , Utheim Tor P. , Hove Lene Hystad , Jensen Janicke Liaaen 
Published: June 2022

AbstractIn the present study, the relationship between dry eyes and dry mouth was explored in 150 65-year-old subjects randomly selected from the general population in Oslo, Norway. The number of drugs, including xerogenic drugs, and current and previous systemic diseases were recorded. Ocular parameters recorded were the McMonnies Dry Eye Questionnaire, the Ocular Surface Disease Index, the Schirmer I Test, tear film break-up time and ocular surface staining. The oral parameters were xerostomia frequency, Summated Xerostomia Inventory, Clinical Oral Dryness Score, and unstimulated and stimulated whole saliva. The participants with current or previous systemic diseases had significantly more ocular and oral symptoms and significantly more oral clinical findings than the participants without a history of disease. Moreover, correlation and factor analyses demonstrated an association between subjective ocular and oral parameters. A significant correlation between the total number of drugs and the presence of ocular and oral symptoms was also noted. When the participants were categorized based on their ocular symptoms, poorer values were found for the oral parameters among the participants more troubled with dry eyes. The results in the present study call for increased awareness and an interdisciplinary approach in matters related to dry eyes and dry mouth.
Dietary supplements do not improve bone morphology or mechanical properties in young female C57BL/6 mice
Authors: Creecy Amy , Smith Collier , Wallace Joseph M. 
Published: June 2022

AbstractBone is a hierarchical material formed by an organic extracellular matrix and mineral where each component and their physical relationship with each other contribute to fracture resistance. Bone quality can be affected by nutrition, and dietary supplements that are marketed to improve overall health may improve the fracture resistance of bone. To test this, 11 week old female C57BL/6 mice were fed either collagen, chondroitin sulfate, glucosamine sulfate, or fish oil 5 times a week for 8 weeks. Femurs, tibiae, and vertebrae were scanned with micro-computed tomography and then mechanically tested. Glucosamine and fish oil lowered elastic modulus, but did not alter the overall strength of the femur. There were no differences in bone mechanics of the tibiae or vertebrae. Overall, the data suggest that dietary supplements did little to improve bone quality in young, healthy mice. These supplements may be more effective in diseased or aged mice.
Ultrathin Al–air batteries by reducing the thickness of solid electrolyte using aerosol jet printing
Authors: Zuo Yuxin , Yu Ying , Feng Junyan , Zuo Chuncheng 
Published: June 2022

AbstractFlexible Al–air batteries have great potential in the field of wearable electronic devices. However, how to reduce the thickness of the battery and improve their applicability in wearable applications is still an unresolved thorny problem. Therefore, this article focuses on the strategies to minimize the thickness of the solid electrolyte for flexible Al–air batteries. In this paper, an innovative aerosol jet printing method is used to prepare the ultrathin neutral electrolyte with a thickness of 18.3–74.5 μm. This study discusses the influence of the thickness and ion concentration on the conductance of the electrolyte in detail. The ultrathin electrolyte has been applied to the flexible Al–air battery, and the battery performance has been explored. The cell pack composed of single cells is light and thin, and can successfully drive small electrical equipment. This study provided new ideas for the preparation of ultrathin electrolyte for flexible energy products.
Beneficial effects of psyllium on the prevention and treatment of cardiometabolic diseases
Authors: Chen Chen , Shang Chang , Xin Laiyun , Xiang Mi , Wang Yuling , Shen Zihuan , Jiao Linke , Ding Fan , Cui Xiangning 
Published: June 2022

Psyllium, especially its husk, has been widely used in food products. This review summarized the potential mechanisms of it in the prevention and treatment of cardiometabolic diseases and its applications in food systems.
Ru@Ni3S2 nanorod arrays as highly efficient electrocatalysts for the alkaline hydrogen evolution reaction
Authors: Wang Kefeng , Li Bin , Ren Jingxiao , Chen Wenxia , Cui Jinhai , Wei Wei , Qu Peng 
Published: June 2022

Ru-decorated Ni3S2 nanorod arrays demonstrate an superior alkaline hydrogen evolution performance. Further modification with polyaniline could significantly enhance the long-term stability for continuous hydrogen generation.
Schlaf, Kindlein, Schlaf…
Authors: Weber Patricia 
Published: June 2022

ZusammenfassungIm Rahmen einer Studie wurde der Einfluss von Musik als Assessmentinstrument
für diagnostische und therapeutische Verfahren in der Endoskopie
untersucht. Hierbei wurde vor allem die Auswirkung auf den Gemütszustand
und die Vitalparameter der Patienten betrachtet. Durch analytische Mittel wurden
die Ergebnisse der Studie in Form von Tabellen und Diagrammen ausgewertet, um
Rückschlüsse auf den Erfolgsfaktor der musikalischen Anwendung
während einer Untersuchung zu rechtfertigen, und um dadurch einen festen
Einsatz von Musik in der Endoskopie zu etablieren.
Interprofessionelles Wissens- und Skillstraining der PEG-Anlage an
Simulatoren – Entwicklung und Testung eines nationalen Curriculums
für Ärzte und spezialisierte Pflegende
Authors: Engelke Monika , Grund Karl Ernst , Schilling Dieter , Beilenhoff Ulrike , Kern-Waechter Elisabeth , Engelke Olaf , Stebner Ferdinand , Kugler Christiane 
Published: June 2022

Zusammenfassung
Einleitung Ein Positionspapier verschiedener Fachgesellschaften zur
Delegation ärztlicher Tätigkeiten in der Gastroenterologie
fordert im Abschnitt „Perkutane Punktion bei der Anlage einer perkutanen
endoskopischen Gastrostomie (PEG)“ ein nationales Curriculum für
Trainingskurse, die vor der Durchführung der Punktion absolviert werden
sollen.Gegenstand der vorliegenden Studie ist die Entwicklung und Testung eines solchen
Curriculums. Ziel ist die Vermittlung des notwendigen Wissens und der Skills zur
sicheren Durchführung der PEG-Anlage für Ärzte und
Pflegende.
Methoden Die Testung erfolgte im Rahmen einer nicht randomisierten,
interventionellen Pilotstudie an Simulatoren. Sie umfasste (1) eine
Vorbereitungsphase, (2) eine theoretische und (3) eine praktische Phase. Nach
der theoretischen Phase erfolgte eine Überprüfung der kognitiven
Fähigkeiten mittels Wissenstests. Am Ende der praktischen Phase wurde
das Anlageprozedere jedes einzelnen Teilnehmers mehrkanalig auf Video
aufgezeichnet und die sensomotorischen Fähigkeiten wurden mithilfe eines
Assessmentinstruments (AS-PEG) ausgewertet. Abschließend wurde eine
Evaluation mittels Fragebogen zur Zufriedenheit der Teilnehmenden
durchgeführt.
Ergebnisse Sieben Ärzte und 17 Pflegende absolvierten die im
Curriculum definierten Phasen und abschließenden Prüfungen. Beim
Wissenstest wurden durchschnittlich 70,3±3,7 (61–75) von 76
Punkten erreicht. Beim Test zu den erworbenen sensomotorischen
Fähigkeiten wurden durchschnittlich 37,1±2,1 (32–40) von
42 Punkten erzielt. Die Evaluation ergab eine hohe Zufriedenheit der
Teilnehmenden in Bezug auf Inhalte, Lernzielerreichung und erworbene praktische
Fähigkeiten.
Schlussfolgerung Eine Verbreitung des Curriculums wird als sinnvoll und
notwendig erachtet, um theoretische und praktische Kenntnisse für
Ärzte und Pflegende durch das strukturierte interprofessionelle Wissens-
und Skillstraining zu vermitteln und den Pflegenden einen rechtlich
abgesicherten Rahmen zu bieten.
The Relationship Between Baseline Cortisol Levels and Surgery Method
of Primary Bilateral Macronodular Adrenal Hyperplasia
Authors: Gao Jie , Yu Zhongwei , Sun Fukang , Xu Bilin , Zhang Cuiping , Wang Hongping , Lu Jun , Lei Tao 
Published: June 2022

AbstractAim was to explore the associations between baseline cortisol levels and surgery
method of primary bilateral macronodular adrenal hyperplasia (PBMAH). We
retrospectively reviewed the clinical features and management of 30 patients (18
females and 12 males) who were diagnosed with PBMAH in our center between 2005
and 2019. Based on surgery method, we divided the patients into two groups:
unilateral adrenalectomy (UA) group; and bilateral adrenalectomy (BA) group.
Serum cortisol rhythm and 24-hour urinary free cortisol
(UFC/24 h) levels were assayed using chemiluminescence method.
Associations between baseline cortisol levels and BA were assessed using
logistic regression. The predictive value of baseline cortisol levels for BA was
calculated using receiver operating characteristic (ROC) curves. Twenty patients
(66.7%) underwent UAs and ten patients (33.3%) underwent BAs.
After adjusting for age, sex, BMI, SBP, and adrenal volume, the concentrations
of baseline serum cortisol (8 AM, 4 PM, and 0 AM) and UFC/24 h
were associated with bilateral adrenalectomy (all p<0.05). The area
under the ROC curve based on 8 AM serum cortisol level model was larger than
that in models based on 4 PM, 0 AM serum cortisol levels and
UFC/24 h, but the differences were non-significant (all
p>0.05). According to maximum Youden index criteria, the optimal cutoffs
of 8 AM serum cortisol level and UFC were 26.89 μg/dl
and 406.65 μg/24 h, respectively, for BA. The
baseline cortisol levels are positively associated with BA. Increased levels of
baseline cortisol levels may predict higher possibility of BA, which should be
confirmed by prospective studies.
Cluttering in a School-Aged Child: Tackling the Challenges Step by Step
Authors: Scaler Scott Kathleen 
Published: June 2022

AbstractThe purpose of this article is to highlight and provide solutions for the complexities of the communication disorder of cluttering. The article includes a 12-part case study of a school-aged child who is referred for an evaluation due to decreased speech intelligibility. The case is woven throughout the article to illustrate all aspects of client management: initial referral, evaluation and differential diagnosis, treatment, and discharge. The case reflects the challenges of increasing client awareness and the importance of advocating for client's needs throughout the process. This article provides background on myths about cluttering and current research findings to debunk these myths. Additionally, methods for evaluation, differential diagnosis, and treatment of cluttering are presented. An indirect approach to increasing client awareness and family and client education is included. The overall focus is to help the clinician better understand how to evaluate, treat, discharge, and advocate for clients with cluttering in ways that meet clients where they are.