bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations of antibodies to benzo[a]pyrene, estradiol and progesterone with estrogen receptor phenotype in breast cancer tissue
Authors: Polenok E. G. , Mun S. A. , Gordeeva L. A. , Kostyanko M. V. , Antonov A. V. , Verzhbitskaya N. E. , Kolpinskiy G. I. , Glushkov A. N. 
Published: April 2022

Aim. To reveal the associations of IgA antibodies to benzo[a]pyrene, estradiol and progesterone (IgA-Bp, IgA-Es, IgA-Pg) with the conversion of estrogen-receptor positive (ER+) into estrogen-receptor negative (ER-) tumors during breast cancer progression.Materials and Methods. Having collected serum samples from 338 healthy volunteers and 1407 breast cancer patients, we have profiled them for IgA-Bp, IgA-Es, IgA-Pg by means of enzyme-linked immunosorbent assay. Conjugates of bovine serum albumin with Bp, Es and Pg were used as adsorbed antigens and anti-human IgA horseradish peroxidase-conjugated antibodies were used for the detection of specific antigen-bound antibodies. Individual IgA-Bp/IgA-Pg and IgA-Es/IgA-Pg ratios were calculated. Estrogen receptor phenotype was determined using immunohistochemistry.Results. Low IgA-Bp/IgA-Pg ratios (≤ 1) in combination with low IgA-Es/IgA-Pg ratios (≤ 1) indicative of protective immunophenotype were more frequently revealed in healthy women (43.8%) in comparison with stage 1 breast cancer patients with ER+ (12.9%) and ER- (23.9%) tumors. High IgA-Bp/IgA-Pg ratios (>1) with high IgA-Es/IgA-Pg ratios (>1) suggestive of pro-carcinogenic immunological phenotype were less often detected in healthy women (27.5%) as compared with stage 1 breast cancer patients with ER+ (65.5%) and ER- (58.7%) tumors. Prevalence of protective and pro-carcinogenic phenotypes significantly differed in stage 1breast cancer patients with ER+ and ER- tumor phenotypes (p = 0.017). ER- tumor phenotype was more prevalent at II-IV tumor stages (25.6%) than at the stage 1 (16.3%). Conversion of ER+ to ER- tumors reflecting the breast cancer progression was characteristic for the patients with pro-carcinogenic immunological phenotype (p<0.0001).Conclusion. Detection of antibodies against Bp, Es and Pg may be applied as a risk marker of breast cancer development and progression.
Rubidium intake in the adult population of the Omsk region
Authors: Brusentsova A. V. , Turchaninov D. V. , Sokhoshko I. A. , Shirinskiy V. A. 
Published: April 2022

Aim. To assess the nutritional consumption of rubidium in the adult population of the Omsk Region.Materials and Methods. Here we performed a cross-sectional study which included 421 individuals (177 men and 244 women) aged 18 to 83 years (median age 37 (23; 57) years). Rubidium consumption was determined by analysing the frequency of food intake and chemical composition of food consumed by the population of the Omsk Region.Results. Median daily rubidium intake was 1.1 mg/day (0.81; 1.48) that sufficiently exceeds minimum recommended dose (0.1 mg/day). We defined the reference range of rubidium intake as 1.1 (0.71 – 1.71) mg/day. Rubidium consumption tended to increase along with the population age, albeit no significant differences have been found between different age groups as well as between men and women. The most abundant rubidium sources were vegetables (55.6%) and beverages (29.6%). Among vegetables, the main sources of rubidium were onion (31.7%) and fresh tomatoes (20.7%), while tea was responsible for the majority (84.6%) of rubidium consumed from beverages. The proportion of vegetable-derived rubidium increased with age, in contrast to beverage-derived rubidium which demonstrated an opposite trend.Conclusion. The reference range of rubidium intake in the Omsk Region is 1.1 (0.71 – 1.71) mg/day. Rubidium intake does not depend on age and gender. The main sources of rubidium are vegetables and beverages.
Oral Health Policy in Latin America: Challenges for Better Implementation
Authors: Galante Mariana Lopes , Otálvaro-Castro Gabriel Jaime , Cornejo-Ovalle Marco Antonio , Patiño-Lugo Daniel Felipe , Pischel Nicole , Giraldes Amanda Iida , Carrer Fernanda Campos de Almeida 
Published: April 2022

Background: Oral health is considered an important public policy. This study aimed to understand which barriers and facilitator occurs during the implementation of oral health programs/policies.
Methods: A qualitative study was conducted within personal interviews with decision-makers, at the political, technical, and academic levels. Data were transcribed and analyzed using Maxqda®.
Results: The formation of dentists with an emphasis on the public service proved to be a critical knot, also the population´s lack of knowledge about their rights was mentioned. In Chile and Colombia, one of the main facilitators was the previous existence of oral health programs. As barrier, the lack of systematization of epidemiological data. In Brazil, the main facilitators were many dentists available and the political will of politicians to include oral health as public. Managers unprepared to work in public service was one of the main barriers.
Conclusions: During the development and implementation of public policies several factors are encountered, it can be concluded that human resources are fundamental elements for the functioning of public service, besides the interest of the managers and politicians for the inclusion of this theme in the political agenda of the country.
Factors that Influence the Clinical Longevity of Ceramic Unit Restoration: A Scoping Review
Authors: Sousa N. O. , Galvão C. S. , Almeida E. O. , Freitas Júnior A. C. 
Published: April 2022

Statement of Problem: Ceramic restorations can be essential in dental rehabilitation aesthetic and functional, but its longevity and treatment plan need to be good settled down. Different material options, manufacturing techniques and protocols are available. It is important to map the literature to know the state of the art about of clinical failures related to these fixed prostheses.
Purpose: Evaluate the rate of survival of single restorations (crowns and ceramic veneers) as well as identify the types of clinical complications reported.
Material and methods: A scoping review was conducted from the MEDLINE/Pubmed, Embase, Scopus, Web of Science and Cochrane Library. A search of all published literature until June 2021 was based on the following keywords: ('dental crown' OR 'ceramic veneer' OR 'porcelain veneer' OR 'dental veneer' OR 'dental porcelain') AND ('survival rate' OR 'ceramic restoration failure' OR 'prosthesis complication' OR complication).
Results: The most reported biological complications were need for treatment endodontic and detachment. The complications and technical failures involved, mainly, fractures and cracks in ceramics. There were also cases of pigmentation marginal.
Conclusion: The survival rate of single ceramic restorations is high. The main factors influencing restoration failures are bruxism, operator inexperience and inadequate cementation.
Evaluating the Impact of Small Farmer’s Inclusion in Agricultural Value Chain for Sustainable Rural Development in Albania
Authors: Tomorri Ilir , Keco Remzi , Tomorri Kejsi 
Published: April 2022

The rural sector is one of the most important and largest sector in Albania, referring to the contribution to economic growth, gross domestic product, level of employment in the country and increasing the welfare of the population living in rural areas. The average small farm sizes, combined with the excessive land fragmentation, the low level of investments, poor infrastructure, and the loss of agricultural land due to the change of use, have been major structural challenges for the agriculture sector in Albania. The farm structures are dominated by smallholders with many small farms (1.2 ha/farm) and few large farms. Farmers and smallholders are critical for food security, as well as for poverty reduction of rural areas. The smallholder farmers facing a number of challenges such as lack of access to reliable and timely data on market prices as well as markets, inability to access appropriate infrastructure such as storage facilities leading to post-harvest losses or low prices as a result of low-quality produce. One way to counter the above challenges is through the agricultural value chain. If a strong market linkage is created, then it can lead to the development of the value chains, and stimulate an increase in the productive base of the smallholders. An effective market linkage increases opportunities for value addition which can benefit the farmers as well as the smallholders.
This paper is intended to highlight the importance of farmers and smallholders in the value chain as a key to sustainable development for the rural sector.
Rośliny lecznicze terenów odłogowanych Pogórza Przemyskiego
Authors: Stokłosa Agnieszka , Stępnik Katzarzyna , Barabasz-Krasny Beata 
Published: April 2022

W pracy przestawiono charakterystykę i rozmieszczenie roślin leczniczych Pogórza Przemyskiego. Badania przeprowadzono w sierpniu 2005 r., w miejscowościach: Leszczyny, Sopotnik, Paportno, Huwniki (południowo-wschodnia część Pogórza) oraz Olszany (północna część Pogórza). Badania przeprowadzono wzdłuż 21 transektów, sporządzając listy roślin leczniczych na 100 poletkach, każde o powierzchni 100 m2. Ilościowość roślin określono na podstawie skali Braun-Blanqueta. Na badanym terenie zlokalizowano 154 taksony flory leczniczej. Większość gatunków (107–70%) wystąpiła w częstości przyjętej jako rzadka (9–16 poletek) i bardzo rzadka (1–8 poletek). Wśród form życiowych taksonów leczniczych dominowały hemikryptofity (82–54%). Gatunki rodzime – apofity, stanowiły większość spośród grup geograficznohistorycznych (142–92%).
Wybrane elementy biologii kiełkowania stulichy psiej (Descurainia sophia Webb. ex Prantl.) i stokłosy żytniej (Bromus secalinus L.)
Authors: KAPELUSZNY JAN , HALINIARZ MAŁGORZATA 
Published: April 2022

Modifications of management ways on cultivated fields entail changes in the species and quantitative composition of segetal flora. The occurrence of such species as Descurainia sophia and Bromus secalinus in crop canopies is one of those effects of changes effect. First, germination ability of their diaspores depending on the age, depth in the soil and light conditions was studied.
The study was carried out at the Department of General Plant Cultivation and Farming, at ambient temperature (about 20°C). Germination ability of flaxweed was examined in 2000–2002, that of rye brome – in two series: autumn and spring. 2006–2007. One part of sown diaspores was placed under natural light (with no additional light); the other was hermetically covered with aluminum foil.
Age and depth of seeds in the soil was another experimental factor. Studies revealed that flaxweed and rye brome seeds germinated both in natural light and in darkness. Germination ability of both species significantly depended on their sowing depth. Descurainia sophia and Bromus secalinus germinated best when diaspores were placed on the soil surface (0.0 cm). In the case of flaxweed, the age of seeds (1–3 years) stored in the soil only slightly affected their germination, while 18-years-old kernels of rye brome did not germinate.
Methods for Determination of Meropenem Concentration in Biological Samples
Authors: Rancic Aleksandar 
Published: April 2022

Abstract
Measuring the concentration of antibiotics in biological samples allow implementation of therapeutic monitoring of these drugs and contribute to the adjustment of the dosing regimen in patients. This increases the effectiveness of antimicrobial therapy, reduces the toxicity of these drugs and prevents the development of bacterial resistance. This review article summarizes current knowledge on methods for determining concentration of meropenem, an antibiotic drug from the group of carbapenems, in different biological samples. It provides a brief discussion of the chemical structure, physicochemical and pharmacokinetic properties of meropenem, different sample preparation techniques, use of apparatus and equipment, knowledge of the advantages and limitations of available methods, as well as directions in which new methods should be developed. This review should facilitate clinical laboratories to select and apply one of the established methods for measuring of meropenem, as well as to provide them with the necessary knowledge to develop new methods for quantification of meropenem in biological samples according to their needs.
ANALISIS WASTE MENGGUNAKAN METODE VALUE STREAM ANALYSIS TOOLS PADA PROYEK PEMBANGUNAN INSTALASI GAWAT DARURAT RSUD PIDIE JAYA
Authors: Situmeanga Sheragizca Yolanda , Afifuddin Muhammad , Rani Hafnidar A. 
Published: April 2022

Construction project is a long process activity where the researcher finds obstacles which cannot be separated from the used resources. Everything in the projects which may add the needed costs called by the term waste. In the project implementation of Regional Public Hospital (IGD) construction project in Pidie Jaya, there was a significant delay in the project schedule which could be caused by several things which may lead the project into waste construction. This is the main reason why does the Value stream analysis tools (VALSAT) method was used during the research in order to identify the cause of occuring waste. This goal of this research is to identify the waste which potentially occure during the Regional Public Hospital (IGD) project in Pidie Jaya. Besides, this research is also determine the grade and tool waste by using the VALSAT. Based on the literature review, there are 8 wate categories which could be reviewed based on materials, labor and tools used during the project. The identified waste data are obtained by the interview conducted to 4 respondents and questionaire with 15 respondents and handed out to contratctors. Based on the result and data processing which using descriptive analysis, it can be concluded that the most influential waste on the project was waste waiting and waste overprocessing which obtain a value of 5 and cause the excessive effects on the project. Furthermore, the result of the grade analysis and tool waste determination by using the VALSAT method produced a process Acivity Mapping to analyze the occuring waste that occurs with a total score of 135.
Caracterización fisicoquímica y biológica de los lixiviados procedentes del sitio de disposición final no controlado en Tlapacoyan, Veracruz, México
Authors: López Ramírez Miguel Angel , Aburto Gutiérrez Yasser Armando , Ocaña Drouaillet Keila Elena , Olaya Pirene Castellanos Onorio , Reynoso Fabiola Lango , Ramírez Maribel López 
Published: April 2022

Uno de los principales problemas en México es la contaminación por lixiviados que se producen en los sitios de disposición final de residuos ya que son altamente tóxicos y dañan a la biota. Esta investigación se centra en determinar las características del lixiviado producido en el sitio de disposición final no controlado del municipio de Tlapacoyan, Veracruz (México). Como resultado, se determinó que los parámetros que producen daño a la biota son nitrógeno total (152.759 mg L-1), mercurio (0.006 mg L-1), cadmio (0.003 mg L-1), cobre (0.052 mg L-1), cromo (0.0731 mg L-1), níquel (0.0524 mg L-1), plomo (0.005 mg L-1) y coliformes totales (>2 400 NMP), al no cumplir al menos una de las normas nacionales e internacionales de límites máximos permisibles. Asimismo, se determinó que el lixiviado producido en este sitio es del tipo maduro y poco biodegradable (DBO/DQO=0.018), por lo que los métodos típicos o de biorremediación no son recomendables para su tratamiento
Psychosocial Problems of Rape among Secondary School Students as Perceived by Parents in Ilorin South of Kwara State
Authors: Akunna Agubosi Lydia , Ajokpaniovo Michael , Gbenga Adegbite Tobi 
Published: April 2022

This study examined the psychosocial problems of rape in Ilorin South of Kwara State. A descriptive survey design was employed in the study. The population consisted of parents in Ilorin South of Kwara State, with a sample of two hundred and twenty respondents being selected through a multistage sampling method. A researcher designed instrument tagged ‘Psychosocial Problems of Rape Questionnaire (PPRQ)’ was used to collect data. The instrument was well validated by experts in test and measurement. Reliability was ascertained through a test retest method which yielded a reliability co-efficient of 0.77. One research question and four hypotheses guided the study. Data was analysed with mean and rank order, t-test and Analysis of variance. Hypotheses were tested at a 0.05 level of significance. Findings revealed that the psychosocial problems of rape include: an inferiority complex, a feeling of being unsafe, a low self-esteem, depression, emotional stress, social withdrawal, among other problems. Findings also showed that age, gender and religion did not influence the respondents or their responses on the problems of rape, while they differed in their responses based on their educational level. Based on the findings, it was recommended as follows: government should set up serious measures to tackle incidences of rape in schools and punish the rapists severely, and schools should, through counselling, educate the students on how to avoid being victims of rape. Schools should also be fenced all round.
In-silico Studies of Phytochemicals of Ashwagandha, Harsingar, Meethi neem and Tulsi Against Covid-19
Authors: Anand Vandita , Srivastava Saumya , Pandey Anjana 
Published: April 2022

Abstract. The ongoing coronavirus disease 2019 (COVID-19) has become a global pandemic and risk to the healthcare system of almost every nation around the world. The endocytic pathway has been considered as a key factor in viral infection. In the case of CoVs, several investigations have shown that these viruses mainly follow the clathrin-mediated endocytic pathway. As a result, inhibiting the clathrin-mediated endocytic pathway might be a useful therapeutic approach. In this study, bioactive components of Harsingar, Meethi neem, Tulsi and Ashwagandha extract was analyzed by HR-LCMS and among them 55 phytochemical compounds were selected based on antiviral and steroidal properties. 55 phytochemical compounds of four Indian herbal plants were used to analyze their binding with clathrin protein associated with COVID -19. Based on the molecular docking as well as ADMET analysis, Ashwagandha, Harsingar, Meethi neem and Tulsi were identified as potential herbal medicine candidates. We have found that the inhibition potentials of the Ashwagandha, Harsingar, Meethi neem and Tulsi are very promising with no side effects.

Resumen. La enfermedad provocada por el coronavirus 2019 (COVID-19) se ha convertido en una pandemia global y pone en riesgo a los sistemas de salud de casi cualquier nación en el mundo. Se ha considerado que la ruta endocítica es un factor clave en la infección viral. En el caso de CoVs, varias investigaciones han mostrado que estos virus siguen la ruta endocítica mediada por la clatrina. Como resultado, inhibir la ruta endocítica mediada por la clatrina puede ser una propuesta terapéutica útil. En este estudio, se analizaron extractos de componentes bioactivos de Harsingar, Meethi neem, Tulsi y Ashwagandha por HR-LCMS y entre ellos se seleccionaron 55 compuestos fitoquímicos basados en sus propiedades antivirales y esteroidales. Estos 55 compuestos obtenidos de 4 plantas herbáceas se utilizaron para analizar su interacción con la proteína clatrina asociada al COVID-19. Basados en el acoplamiento molecular así como en el análisis ADMET, se determinó que Harsingar, Meethi neem, Tulsi y Ashwagandha son candidatos potenciales de medicinas herbáceas. Hemos encontrado que los potenciales de inhibición de Harsingar, Meethi neem, Tulsi y Ashwagandha son muy promisorios y no muestran efectos colaterales.
Selective Pb(II)-Imprinted Polymer for Solid Phase Extraction in the Trace Determination of Lead in Infant Formula by Capillary Electrophoresis
Authors: Flores-Aguilar Juan Francisco , Islas Gabriela , Rodríguez José Antonio , Paez-Hernandez María Elena , Galan-Vidal Carlos Andrés , Ibarra Israel Samuel 
Published: April 2022

Abstract. A lead ion-imprinted polymer (IIP) based on methacrylic acid (MAA) and 4-vinylpyridine (4VP) as functional monomers has been synthesized by a radical precipitation method. The complex Pb-MAA-4VP was polymerized with ethylene glycol dimethacrylate as the cross-linker agent. The material was used in solid phase extraction (SPE) coupled with capillary electrophoresis (CE) for the determination of lead in infant formula samples. The physical-chemical properties of the IIP were characterized by scanning electron microscopy, IR spectroscopy, and Freundlich and Dubinin-Raduskevich models. The IIP selectivity was determined in presence of Ca(II), Mg(II), Cd(II), Cr(III) and Cu(II), which are present in the infant formula sample. The proposed methodology (SPE-CE) for the determination of lead has a limit of detection of 0.5 µg L-1 and a limit of quantification of 1.5 µg L-1, with an intra and inter-day repeatability of less than 5 % RSD in all cases. Four of twenty samples were positive for lead with concentrations ranging from 10 to 43 µg kg-1, and the results were compared with ETAAS. ANOVA demonstrated no significant difference between the results obtained by SPE-CE and ETAAS (α = 0.05 %).

Resumen. Se realizó la síntesis de un polímero impreso de iones (IIP) vía radicales libres para la extracción efectiva de plomo, empleando como monómeros funcionales ácido metacrilico (MAA) y 4-vinilpiridina (4VP). El complejo Pb-MAA-4VP fue polimerizado empleando al dimetacrilato de etilenglicol como agente entrecruzante. El polímero fue utilizado en un sistema de extracción en fase sólida (SPE) acoplado a electroforesis capilar (CE) en la determinación de plomo en muestras de fórmulas infantiles. El IIP óptimo fue caracterizado respecto a su morfología y propiedades fisicoquímicas mediante microscopia electrónica de barrido, espectroscopia IR e isotermas de adsorción (modelo Freundlich y Dubinin-Raduskevich). La selectividad del IIP se estudió en presencia de iones metálicos contenidos en las fórmulas infantiles tales como: Ca(II), Mg(II), Cd(II), Cr(III) y Cu(II). La metodología propuesta en el presente trabajo (IIP-SPE-CE) mostró límites de detección de 0.5 µg L-1 y límites de cuantificación de 1.5 µg L-1, con una repetitividad y reproducibilidad adecuadas (< 5 % DER) en todos los casos. Cuatro de las veinte muestras analizadas dieron positivo a la presencia de plomo con concentraciones entre 10 a 43 µg L-1, dichos resultados fueron comparados con una metodología de referencia (ETAAS). El análisis de ANOVA demostró que no existe diferencia significativa entre los resultados obtenidos por IIP-SPE-CE y ETAAS (α=0.05 %).