bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementing a cost effective and configurable hybrid simulation platform in healthcare education, using wearable and web-based technologies
Authors: Brown Wayne J. , Reid Cindy 
Published: May 2022

AbstractThere are many examples of hybrid simulation models in healthcare education which are designed to simulate specific scenarios. However, there appears to be a need for a cost effective and configurable hybrid simulation platform which can be used by educators of various healthcare disciplines to simulate different scenarios. The purpose of this paper is to develop a proof-of-concept platform that can be easily implemented at little cost and provide flexibility to healthcare instructors to develop a variety of simulation scenarios, and to determine the effectiveness of this platform. Using a standardized patient, a person acting as a patient in a scripted manner, along with wearable and web-based technologies, a congestive heart failure simulation was used as an evaluative exercise for a group of personal support worker students at a Canadian Community College. Personal support workers typically provide care to any person who may require personal assistance with activities of daily living such as feeding, lifting, bathing, skin care and oral hygiene to name a few. Standardized patients are typically used in healthcare education to educate and evaluate soft skills, such as caregiver to patient communication, professionalism, as well as hard skills, such as history taking, examination and diagnostic skills (Rosen in J Crit Care 23:157–166, 2008). Instructor feedback indicated that the platform was easy to use and capable of simulating a large variety of scenarios. Pre and post test results are evidence of initial findings of promise indicating that the platform seemed to be effective in enabling students to meet learning outcomes. Focus group results seem to indicate an increase in student confidence as it relates to their ability to handle a similar scenario in the workplace.
Effects of vertical nonlinearity on the superconducting gravimeter CT #036 at Ishigakijima, Japan
Authors: Imanishi Yuichi , Nawa Kazunari , Tamura Yoshiaki , Ikeda Hiroshi 
Published: May 2022

AbstractOne of the characteristic features of the gravity recordings produced by the superconducting gravimeter CT #036 at Ishigakijima, Japan, is that it indicates gravity increase when a typhoon (hurricane) approaches the island. Since we are trying to detect small gravity signals associated with the long-term slow slip events in this region, it is very important in the interpretation of the observed data whether such gravity changes are of natural or instrumental origin. In this paper, we investigate whether or not nonlinearity in the sensor of the superconducting gravimeter is responsible for this phenomenon. Here we take the same theoretical approach as taken by our previous study which investigated the effect of coupling between horizontal and vertical components of the gravity sensor in order to understand the noise caused by the movements of a nearby VLBI antenna. From theoretical and experimental approaches, we prove that the gravity increase observed by CT #036 at the times of high background noise level cannot be explained by instrumental effects, such as the nonlinearity in the vertical component or the coupling between horizontal and vertical components of the gravity sensor. This implies that the observed gravity increases are real gravity signals of natural origin.
Graphical Abstract
Restoration of the patient-specific anatomy of the distal fibula based on a novel three-dimensional contralateral registration method
Authors: Calek Anna-Katharina , Hodel Sandro , Hochreiter Bettina , Viehöfer Arnd , Fucentese Sandro , Wirth Stephan , Vlachopoulos Lazaros 
Published: May 2022

Abstract
Purpose
Posttraumatic fibular malunion alters ankle joint biomechanics and may lead to pain, stiffness, and premature osteoarthritis. The accurate restoration is key for success of reconstructive surgeries. The aim of this study was to analyze the accuracy of a novel three-dimensional (3D) registration algorithm using different segments of the contralateral anatomy to restore the distal fibula.

Methods
Triangular 3D surface models were reconstructed from computed tomographic data of 96 paired lower legs. Four segments were defined: 25% tibia, 50% tibia, 75% fibula, and 75% fibula and tibia. A surface registration algorithm was used to superimpose the mirrored contralateral model on the original model. The accuracy of distal fibula restoration was measured.

Results
The median rotation error, 3D distance (Euclidean distance), and 3D angle (Euler’s angle) using the distal 25% tibia segment for the registration were 0.8° (− 1.7–4.8), 2.1 mm (1.4–2.9), and 2.9° (1.9–5.4), respectively. The restoration showed the highest errors using the 75% fibula segment (rotation error 3.2° (0.1–8.3); Euclidean distance 4.2 mm (3.1–5.8); Euler’s angle 5.8° (3.4–9.2)). The translation error did not differ significantly between segments.

Conclusion
3D registration of the contralateral tibia and fibula reliably approximated the premorbid anatomy of the distal fibula. Registration of the 25% distal tibia, including distinct anatomical landmarks of the fibular notch and malleolar colliculi, restored the anatomy with increasing accuracy, minimizing both rotational and translational errors. This new method of evaluating malreductions could reduce morbidity in patients with ankle fractures.

Level of evidence
IV
Celastrol mitigates inflammation in sepsis by inhibiting the PKM2-dependent Warburg effect
Authors: Luo Piao , Zhang Qian , Zhong Tian-Yu , Chen Jia-Yun , Zhang Jun-Zhe , Tian Ya , Zheng Liu-Hai , Yang Fan , Dai Ling-Yun , Zou Chang , Li Zhi-Jie , Liu Jing-Hua , Wang Ji-Gang 
Published: May 2022

Abstract
Background
Sepsis involves life-threatening organ dysfunction and is caused by a dysregulated host response to infection. No specific therapies against sepsis have been reported. Celastrol (Cel) is a natural anti-inflammatory compound that shows potential against systemic inflammatory diseases. This study aimed to investigate the pharmacological activity and molecular mechanism of Cel in models of endotoxemia and sepsis.

Methods
We evaluated the anti-inflammatory efficacy of Cel against endotoxemia and sepsis in mice and macrophage cultures treated with lipopolysaccharide (LPS). We screened for potential protein targets of Cel using activity-based protein profiling (ABPP). Potential targets were validated using biophysical methods such as cellular thermal shift assays (CETSA) and surface plasmon resonance (SPR). Residues involved in Cel binding to target proteins were identified through point mutagenesis, and the functional effects of such binding were explored through gene knockdown.

Results
Cel protected mice from lethal endotoxemia and improved their survival with sepsis, and it significantly decreased the levels of pro-inflammatory cytokines in mice and macrophages treated with LPS (P < 0.05). Cel bound to Cys424 of pyruvate kinase M2 (PKM2), inhibiting the enzyme and thereby suppressing aerobic glycolysis (Warburg effect). Cel also bound to Cys106 in high mobility group box 1 (HMGB1) protein, reducing the secretion of inflammatory cytokine interleukin (IL)-1β. Cel bound to the Cys residues in lactate dehydrogenase A (LDHA).

Conclusion
Cel inhibits inflammation and the Warburg effect in sepsis via targeting PKM2 and HMGB1 protein.
Influence of irradiation distance on the mechanical performances of resin composites polymerized with high-irradiance light curing units
Authors: Oh Soram , Kim Hyun Ju , Kim Hyun-Jung , Antonson Sibel A. , Kim Sun-Young 
Published: May 2022

Abstract

Background
The aim of this study was to evaluate the influence of increased irradiation distance on the flexural strength (FS), dentin micro-shear bond strength (μSBS), and the degree of conversion (DC) of bulk-fill flowable, conventional flowable, and packable resin composites.

Methods

The resin composites tested were Surefil® SDR™ (SDR), Filtek Z350 XT Flowable Restorative A2 shade (Z3F), and Filtek Z350 XT Universal Restorative A2 shade (Z3P). Specimens were cured at four irradiation distances (0, 2, 4, and 8 mm) with an Elipar DeepCure-S LED curing light for 20 s. FS tests were performed (
n
= 15) using bar-shaped specimens (8 mm × 2 mm × 2 mm) of the resin composites. μSBS tests were performed on the occlusal surfaces of extracted third molars from humans that were ground to expose dentin (
n
= 15). DC was measured by using Raman spectroscopy on the top and bottom surfaces of disk specimens (2-mm thick) (
n
= 3). To further investigate whether extended irradiation times could compensate for reduced irradiance, additional Z3P specimens were prepared, which were light-cured at 8-mm distances for 40 and 60 s and subjected to FS tests, μSBS tests, and Raman spectroscopy. Both two-way and one-way ANOVA were used for statistical analyses.

Results

Both FS and DC of Z3P specimens cured at an 8-mm distance were significantly lower than those cured at shorter distances (
p
< 0.05), whereas the FS and DC of the Z3F and SDR specimens were not significantly influenced by increasing distances. The μSBSs of the three types of resin composites reduced with increasing irradiation distances. The FS, μSBS, and DC of the Z3P specimen light-cured at 8 mm for 40 s were comparable to those of the Z3P specimen cured at 0 mm for 20 s.

Conclusions
Increasing the irradiation distance to 8 mm can have a deleterious influence on mechanical performances, including the FS, DC, and dentin μSBS, of the resin composites polymerized with high-irradiance light curing units.
Insights into AIM-InDel diversities in Yunnan Miao and Hani ethnic groups of China for forensic and population genetic purposes
Authors: Cui Wei , Nie Shengjie , Fang Yating , Chen Man , Zhao Ming , Lan Qiong , Shen Chunmei , Zhu Bofeng 
Published: May 2022

Abstract
Background
Ancestry informative markers are regarded as useful tools for inferring the ancestral information of an individual, which have been widely used in the criminal investigations and population genetic studies. Previously, a multiplex amplification panel containing 39 AIM-InDel loci was constructed. This study aims to investigate the genetic polymorphisms of these 39 AIM-InDel loci in Yunnan Hani and Miao ethnic groups, and to uncover their genetic affinities with reference populations based on the AIM-InDel markers.

Materials and methods
In this research, 39 AIM-InDel profiles of 203 unrelated Miao individuals and 203 unrelated Hani individuals in Yunnan province of China were acquired. Additionally, we evaluated the genetic polymorphisms of 39 InDel loci in Yunnan Miao and Hani groups. Moreover, the genetic relationships among Yunnan Miao, Hani and reference populations were also clarified based on Nei’s genetic distances, pairwise fixation indexes, principal component analyses, phylogenetic analyses, and STRUCTURE analyses.

Results
Genetic diversity analyses demonstrated that these InDel loci showed varying degrees of genetic polymorphisms, and could be utilized in forensic identifications in Yunnan Miao and Hani groups. The results of principal component analyses, phylogenetic analyses and Structure analyses revealed that Yunnan Miao and Hani groups had closer genetic relationships with East Asian populations, especially with the populations from Southern China. This research enriched the genetic data of Chinese ethnic minority, and provided ancestral information of Yunnan Miao and Hani groups from the perspective of population genetics.
Modified Hawking temperature and entropy of general stationary black holes by Lorentz invariance violation
Authors: Li Ran , Ding Qun-Tao , Yang Shu-Zheng 
Published: May 2022

Abstract
Correction of Lorentz dispersion relation can be carried out through Lorentz invariance violation (LIV). In the curved space-time with the general stationary black holes, the fermions dynamic equations have been modified more accurately according to the modified Lorenz dispersion relation, and the equations have been solved to obtain a new expression of quantum tunneling rate, black hole Hawking temperature, black hole entropy, and other physical quantities of stationary black holes. These expressions not only are related to factors such as Lorentz invariance violation but also have more contents akin to black hole physics.
Characteristics and Outcomes of Discharge Against Medical Advice and 30-Day Readmissions After Concussion: Analysis of the Nationwide Readmissions Database
Authors: Jumah Fareed , Chotai Silky , Hilden Patrick , Raju Bharath , Nagaraj Anmol , Agarwalla Pankaj , Johnson Stephen , Gupta Gaurav , Sun Hai , Nanda Anil 
Published: May 2022

BACKGROUND:
Little is known about the impact of discharge against medical advice (DAMA) in patients admitted with concussion.

OBJECTIVE:
To explore the prevalence of DAMA and its effect on 30-day readmissions and cost in concussion using a nationally representative sample.

METHODS:
The Nationwide Readmissions Database was queried for concussion admissions and their disposition at discharge between 2010 and 2014. Included patients were ≥18 years old who were admitted with concussion and had <1 hour loss of consciousness. We excluded in-hospital deaths or discharge dispositions other than home or against medical advice. Univariate and multivariate analyses were performed to determine characteristics associated with DAMA and 30-day readmissions.

RESULTS:
A total of 38 919 index admissions were identified, which resulted in 998 (2.6%) DAMA. In multivariate analysis, characteristics associated with DAMA included younger age (odds ratio [OR] = 0.84, 95% CI 0.80-0.88), male sex (1.47, 1.22-1.76), an Elixhauser comorbidity index >3 (1.67, 1.15-1.60), and assault (2.02, 1.48-2.75) and fall injuries (1.28, 1.03-1.60). The highest-income quartile was negatively associated with DAMA (0.91, 0.73-1.13). In multivariate analysis, DAMA (1.63, 1.29-2.06), higher comorbidity index (2.61, 2.22-3.06), and self-inflicted mode of injury (2.28, 1.30-4.02) were independently associated with 30-day readmission. The most common indication for readmission in both routine and DAMA groups was traumatic injury (21.1% and 24.5%, respectively).

CONCLUSION:
DAMA is an independent risk factor for readmission in patients admitted for concussion. The variables associated with DAMA identified in this study can be used to design patient-centered interventions that can be implemented to reduce DAMA and its impact on clinical outcomes in patients with traumatic brain injury.
Risk Factors and Neurological Outcomes Associated With Circulatory Shock After Moderate–Severe Traumatic Brain Injury: A TRACK-TBI Study
Authors: Toro Camilo , Hatfield Jordan , Temkin Nancy , Barber Jason , Manley Geoffrey , Ohnuma Tetsu , Komisarow Jordan , Foreman Brandon , Korley Frederick K. , Vavilala Monica S. , Laskowitz Daniel T. , Mathew Joseph P. , Hernandez Adrian , Sampson John , James Michael L. , Raghunathan Karthik , Goldstein Benjamin A. , Markowitz Amy J. , Krishnamoorthy Vijay 
Published: May 2022

BACKGROUND:Extracranial multisystem organ failure is a common sequela of severe traumatic brain injury (TBI). Risk factors for developing circulatory shock and long-term functional outcomes of this patient subset are poorly understood.OBJECTIVE:To identify emergency department predictors of circulatory shock after moderate–severe TBI and examine long-term functional outcomes in patients with moderate–severe TBI who developed circulatory shock.METHODS:We conducted a retrospective cohort study using the Transforming Clinical Research and Knowledge in TBI database for adult patients with moderate–severe TBI, defined as a Glasgow Coma Scale (GCS) score of <13 and stratified by the development of circulatory shock within 72 hours of hospital admission (Sequential Organ Failure Assessment score ≥2). Demographic and clinical data were assessed with descriptive statistics. A forward selection regression model examined risk factors for the development of circulatory shock. Functional outcomes were examined using multivariable regression models.RESULTS:Of our moderate–severe TBI population (n = 407), 168 (41.2%) developed circulatory shock. Our predictive model suggested that race, computed tomography Rotterdam scores <3, GCS in the emergency department, and development of hypotension in the emergency department were associated with developing circulatory shock. Those who developed shock had less favorable 6-month functional outcomes measured by the 6-month GCS-Extended (odds ratio 0.36,P= .002) and 6-month Disability Rating Scale score (Diff. in means 3.86,P= .002) and a longer length of hospital stay (Diff. in means 11.0 days,P< .001).CONCLUSION:We report potential risk factors for circulatory shock after moderate–severe TBI. Our study suggests that developing circulatory shock after moderate–severe TBI is associated with poor long-term functional outcomes.
A non-disruptive and efficient knock-in approach allows fate tracing of resident osteoblast progenitors during repair of vertebral lesions in medaka
Authors: Tan Wen Hui , Winkler Christoph 
Published: May 2022

ABSTRACTDuring bone development and repair, osteoblasts are recruited to bone deposition sites. To identify the origin of recruited osteoblasts, cell lineage tracing using Cre/loxP recombination is commonly used. However, a confounding factor is the use of transgenic Cre drivers that do not accurately recapitulate endogenous gene expression or the use of knock-in Cre drivers that alter endogenous protein activity or levels. Here, we describe a CRISPR/Cas9 homology-directed repair knock-in approach that allows efficient generation of Cre drivers controlled by the endogenous gene promoter. In addition, a self-cleaving peptide preserves the reading frame of the endogenous protein. Using this approach, we generated col10a1p2a-CreERT2 knock-in medaka and show that tamoxifen-inducible CreERT2 efficiently recombined loxP sites in col10a1 cells. Similar knock-in efficiencies were obtained when two unrelated loci (osr1 and col2a1a) were targeted. Using live imaging, we traced the fate of col10a1 osteoblast progenitors during bone lesion repair in the medaka vertebral column. We show that col10a1 cells at neural arches represent a mobilizable cellular source for bone repair. Together, our study describes a previously unreported strategy for precise cell lineage tracing via efficient and non-disruptive knock-in of Cre.
PENINGKATAN PERAN KADER KESEHATAN DALAM DETEKSI DINI RISIKO PENYAKIT KARDIOVASKULER DENGAN JAKARTA KARDIOVASKULER SKOR
Authors: Martiningsih Martiningsih , Wulandari Ade 
Published: May 2022

Penyakit kardiovaskular (PKV) adalah penyakit yang disebabkanÂ oleh gangguan fungsi jantung dan pembuluh darah yang dapat dicegah terutama pada kelompok berisiko. PencegahanÂ perilaku berisiko dan merubahnya menjadi perilaku yang menunjang kesehatan melalui edukasi menjadi penting dalam upaya preventif jangka panjang yang terus berkelanjutan dan bersinergi dengan program yang menunjang kesehatan. Tujuan kegiatan pengabdian kepada masyarakat ini adalah memberikan edukasi deteksi dini risiko PKV bagi kader kesehatan. Kegiatan dilaksanakan di Desa Penapali Kecamatan Woha Kabupaten Bima-NTB pada 19 kader kesehatan, pada hari Sabtu-Minggu, tanggal 26-27 Oktober 2019 berupa penyuluhan kesehatan tentang deteksi dini risiko penyakit kardiovaskuler menggunakan Jakarta kardiovaskuler skor (JKS) melalui media leaflet dilanjutkan demonstrasi pengukuran tekanan darah,tinggi badan, berat badan dan menghitung indeks massa tubuh serta diskusi penggunaan JKS untuk mengetahui besarnya risiko PKV. Karakteristik kader kesehatan yang mengikuti kegiatan penyuluhan semuanya adalah perempuan, pada kelompok usia 21 â€“ 45 tahun. Penilaian risiko pada kader kesehatan menunjukkan hasil semua peserta dapat melakukan pengukuran dan hasil penilaian dengan JKS pada kelompok kader menunjukkan 100% Â kader pada kelompok risiko rendah PKV namun temuan lain mengindikasikan bahwa faktor risiko terbanyak adalah pada indeks massa tubuh melebihi normal (overweigh dan obesitas 55,6%). Kedepannya peran kader perlu ditingkatkan melalui kelas edukasiÂ dan program edukasi Â berkelanjutan.
PENINGKATAN PENGETAHUAN TENTANG MAKANAN PENDAMPING ASI DI DESA DADAPAYU KECAMATAN SEMANU GUNUNGKIDUL
Authors: Ayu Suci Musvita , Pungkasan Pupung - , Novitasari Lia Anjar 
Published: May 2022

Â MP-ASI merupakan makanan terbaik dan utama karena mempunyai kandungan zat gizi yang sangat diperlukan untuk melindungi bayi dari berbagai penyakit terutama penyakit infeksi dan pencegahan dalam kekurangan gizi pada bayi usia 6 bulan sampai 24 bulan. Tujuan pemberian penyuluhan dan pelatihan tentang MP-ASI yaitu untuk meningkatkan tingkat pengetahuan masyarakat terkait pentingnya pemberian MP-ASI.Â Metode yang digunakan adalah dengan memberikan penyuluhan dan pelatihan secara langsung kepada ibu-ibu dengan pemberian pengetahuan dan praktik pembuatan makanan pendamping ASI (MP-ASI) secara langsung. Hasil yang diperoleh adalah kegiatan penyuluhan dan pelatihan berjalan dengan baik dan peserta aktif mengikuti kegiatan penyuluhan dan pelatihan sampai selesai. Penyuluhan dan pelatihan dilakukan dengan interaktif antara narasumber dengan peserta pelatihan.Â
PELATIHAN VERTICAL GARDEN DALAM OPTIMALISASI LORONG SAYUR DI LAHAN SEMPIT KECAMATAN NGAMPILAN YOGYAKARTA
Authors: Utami Inggita , Elisthatiana Yeni , Mustaghfiroh Zulfa , Yunita Vera 
Published: May 2022

Pembangunan di perkotaan berdampak pada semakin sempitnya lahan pertahan maupun lahan pekarangan, sehingga diperlukan inovasi urban farming untuk bercocok tanam di lahan yang sempit. Tujuan pengabdian kepada masyarakat ini adalah memberikan pelatihan pembuatan vertical garden kepada warga Kelurahan Notoprajan Kecamatan Ngampilan Yogyakarta untuk mengoptimalkan lorong sayur di lahan sempit. Kegiatan ini diawali dengan pemberian edukasi dan pelatihan kepada ibu-ibu PKK (Pembinaan Kesejahteraan Keluarga) dan perwakilan RW (Rukun Warga) dan diakhiri dengan perlombaan kreasi vertical garden di tingkat kecamatan. Kegiatan ini dilakukan bekerjasama dengan kader-kader lorong sayur dari DLH (Dinas Lingkungan Hidup) yang berada di wilayah Kecamatan Ngampilan. Hasil dari pelatihan ini adalah adanya peningkatan pengetahuan, keahlian dan partisipasi warga dalam mengolah bahan bekas, seperti botol bekas, helm bekas, pelek sepeda bekas, spanduk bekas menjadi wadah pot yang tersusun vertikal, tertatanya kembali lorong sayur disetiap lahan sempit RT 1 hingga RT 20, serta terpilihnya perwakilan lorong sayur di RW 2 sebagai pemenang juara 1 ditingkat kecamatan.Â Â