bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between Copeptin and Six-Month Neurologic Outcomes in Patients With Moderate Traumatic Brain Injury
Authors: Jeon Jin Pyeong , Kim Seonghyeon , Kim Tae Yeon , Han Sung Woo , Lim Seung Hyuk , Youn Dong Hyuk , Kim Bong Jun , Hong Eun Pyo , Park Chan Hum , Kim Jong-Tae , Ahn Jun Hyong , Rhim Jong Kook , Park Jeong Jin , Kim Heung Cheol , Kang Suk Hyung 
Published: April 2022

BackgroundCopeptin has been reported as a predictive biomarker for the prognosis after traumatic brain injury (TBI). However, most of them were in patients with severe TBI and limited value in predicting outcomes in patients with moderate TBI defined as Glasgow Coma Scale (GCS) score from 9 to 12. We aimed to investigate the predictive value of copeptin in assessing the neurologic outcome following moderate TBI.MethodsPatients were prospectively enrolled between May 2017 and November 2020. We consecutively measured plasma copeptin within 24 h after trauma, days 3, 5, and 7 using ELISA. The primary outcome was to correlate plasma copeptin levels with poor neurologic outcome at 6 months after moderate TBI. The secondary outcome was to compare the prognostic accuracy of copeptin and C-reactive protein (CRP) in assessing the outcome of patient.ResultsA total of 70 patients were included for the final analysis. The results showed that 29 patients (41.4%) experienced a poor neurologic outcome at 6 months. Multivariable logistic regression analysis revealed that increased copeptin (odds ration [OR] = 1.020, 95% CI: 1.005–1.036), GCS score of 9 or 10 (OR = 4.507, 95% CI: 1.266–16.047), and significant abnormal findings on CT (OR = 4.770; 95% CI: 1.133–20.076) were independent risk factors for poor outcomes. Consecutive plasma copeptin levels were significantly different according to outcomes (p &lt; 0.001). Copeptin on day 7 exhibited better prognostic performance than CRP with an area under receiver operating characteristic curve (AUROC) difference of 0.179 (95% CI: 0.032–0.325) in predicting 6-month poor outcomes.ConclusionPlasma copeptin level can be a useful marker in predicting 6-month outcomes in patients with moderate TBI.
Combination of Sildenafil and Ba2+ at a Low Concentration Show a Significant Synergistic Inhibition of Inward Rectifier Potassium Current Resulting in Action Potential Prolongation
Authors: Macháček Martin , Švecová Olga , Bébarová Markéta 
Published: April 2022

Sildenafil (Viagra) is a vasodilator mainly used in the treatment of erectile dysfunction. Atrial or ventricular fibrillation may rarely occur as a side effect during sildenafil therapy. Although changes in inward rectifier potassium currents including IK1 are known to contribute to the pathogenesis of fibrillation, the effect of sildenafil on IK1 has not been studied. In experiments, Ba2+ is used as a specific inhibitor of IK1 at high concentrations (usually 100 µM). Being an environmental contaminant, it is also present in the human body; Ba2+ plasmatic concentrations up to 1.5 µM are usually reported in the general population. This study was primarily aimed to investigate changes of IK1 induced by sildenafil in a wide range of concentrations (0.1–100 µM). Additionally, the effect of combination of sildenafil and Ba2+ at selected clinically-relevant concentrations was tested, at 0.1 µM both on IK1 and on the action potential duration (APD). Experiments were performed by the whole-cell patch-clamp technique on enzymatically isolated rat ventricular cardiomyocytes, mostly at 23°C with the exception of APD measurements which were performed at 37°C as well. Sildenafil caused a significant, reversible, and concentration-dependent inhibition of IK1 that did not differ at −50 and −110 mV. Simultaneous application of sildenafil and Ba2+ at 0.1 µM revealed a massive inhibition of both inward and outward components of IK1 (this synergy was missing at other tested combinations). The combined effect at 0.1 µM (45.7 ± 5.7 and 43.0 ± 6.9% inhibition at −50 and −110 mV, respectively) was significantly higher than a simple sum of almost negligible effects of the individual substances and it led to a significant prolongation of APD at both 23 and 37°C. To our knowledge, similar potentiation of the drug-channel interaction has not been described. The observed massive inhibition of IK1 induced by a combined action of the vasodilator sildenafil and environmental contaminant Ba2+ at a low concentration and resulting in a significant APD prolongation may contribute to the genesis of arrhythmias observed in some patients treated with sildenafil.
The Practical Problem With Carbapenem Testing and Reporting Accurate Bacterial Susceptibilities
Authors: Redell Mark , Tillotson Glenn S. 
Published: April 2022

Background: Antibiotic resistance is an evolving issue which requires constant review. Susceptibility breakpoints are revised in line with new microbiological and pharmacological data. Susceptibility breakpoints for carbapenems and Enterobacterales were revised in response to the rise in resistance and the potential for standard doses of carbapenems to provide the necessary antibiotic exposure and to accurately identify rates of carbapenem resistance.Objectives: This review sought to identify real-world implications associated with lack of testing and reporting current carbapenem breakpoints and potential barriers that may impede implementation of these strategies.Methods: A literature review was conducted using PubMed and Google Scholar electronic databases.Results: The failure to adopt revised breakpoints incurs negative clinical outcomes and carries increased cost implications. However, there were several impediments highlighted which are barriers for laboratories to implement breakpoint updates.Conclusion: Possible practical steps to implement revised breakpoints which apply to carbapenems and Enterobacterales are proposed. The challenge for laboratories is to be aware and implement these changes to provide accurate and relevant susceptibility results for clinicians.
Identifying Acute Cardiac Hazard in Early Drug Discovery Using a Calcium Transient High-Throughput Assay in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Authors: Lu Hua Rong , Kreir Mohamed , Karel Van Ammel , Tekle Fetene , Geyskens Danny , Teisman Ard , Gallacher David J. 
Published: April 2022

Introduction: Early identification of cardiac risk is essential for reducing late-stage attrition in drug development. We adapted the previously published cardiac hazard risk-scoring system using a calcium transient assay in human stem cell-derived CMs for the identification of cardiac risks recorded from the new hiPSC-CM line and investigated its predictivity and translational value based on the screening of a large number of reference and proprietary compounds.Methods: Evaluation of 55 reference drugs provided the translation of various pharmacological effects into a single hazard label (no, low, high, or very high hazard) using a Ca2+-sensitive fluorescent dye assay recorded by -by FDSS/µCell Functional Drug Screening System (Hamamatsu on hiPSC-CM line (FCDI iCell Cardiomyocytes2).Results: Application of the adapted hazard scoring system in the Ca2+ transient assay, using a second hiPS-CM line, provided comparable scoring results and predictivity of hazard, to the previously published scoring approach, with different pharmacological drug classes, as well as screening new chemical entities (NCE’s) using a single hazard label from four different scoring levels (no, low, high, or very high hazard). The scoring system results also showed minimal variability across three different lots of hiPSC-CMs, indicating good reproducibility of the cell line. The predictivity values (sensitivity and specificity) for drug-induced acute cardiac risk for QT-interval prolongation and Torsade de pointes (TdPs) were &gt;95% and statistical modeling confirmed the prediction of proarrhythmic risk. The outcomes of the NCEs also showed consistency with findings in other well-established in vitro and in vivo cardiac risk assays.Conclusion: Evaluation of a large list of reference compounds and internal NCEs has confirmed the applicability of the adaptations made to the previously published novel scoring system for the hiPSC-CMs. The validation also established the predictivity for drug-induced cardiac risks with good translation to other established preclinical in vitro and in vivo assays, confirming the application of this novel scoring system in different stem cell-CM lines for early cardiac hazard identification.
A Newly Isolated Strain of Haematococcus pluvialis GXU-A23 Improves the Growth Performance, Antioxidant and Anti-Inflammatory Status, Metabolic Capacity and Mid-intestine Morphology of Juvenile Litopenaeus vannamei
Authors: Fang HaoHang , Zhuang ZhenXiao , Huang LuoDong , Niu Jin , Zhao Wei 
Published: April 2022

Haematococcus pluvialis can be used as a green additive in aquafeeds due to it contains rich astaxanthin and polyunsaturated fatty acid. In the present study, a newly strain of H. pluvialis GXU-A23 with high concentration of astaxanthin was firstly isolated by a newly culture strategy in our laboratory. In addition, H. pluvialis GXU-A23 was applied in the Litopenaeus vannamei feed for determining whether it has positive effects on the growth performance, antioxidant and anti-inflammatory status, metabolic capacity and mid-intestine morphology of juvenile L. vannamei. Shrimp with 0.63 g approximately initial body weight were fed diets supplemented with/without 50 g/kg H. pluvialis GXU-A23. After 8 weeks feeding intervention, significantly higher growth performance of L. vannamei was obtained in the H. pluvialis GXU-A23 treatment group compared to the control group (p &lt; 0.05). At the same time, L. vannamei fed with H. pluvialis GXU-A23 acquired significantly better antioxidant and anti-inflammatory status than the control group (p &lt; 0.05). In addition, higher RNA expression level of hepatopancreas digestive enzyme, hepatopancreas lipid and glucose metabolic enzymes as well as better mid-intestine morphology were found in the H. pluvialis GXU-A23 treatment group than the control group (p &lt; 0.05). These results indicated that 50 g/kg H. pluvialis GXU-A23 was suitable for the L. vannamei feed, which could improve the growth performance, antioxidant and anti-inflammatory status, metabolic capacity and mid-intestine morphology of juvenile L. vannamei.
Childhood Trauma and Psychosocial Stress Affect Treatment Outcome in Patients With Psoriasis Starting a New Treatment Episode
Authors: Wintermann Gloria-Beatrice , Bierling Antonie Louise , Peters Eva M. J. , Abraham Susanne , Beissert Stefan , Weidner Kerstin 
Published: April 2022

ObjectiveTraumatic childhood experiences and psychosocial stress may predispose the evolvement of somatic diseases. Psoriasis is a multifactorial chronic inflammatory skin disease that often associates with current and past stress. Both may entail pathological alterations in major stress axes and a balance shift in the level of T helper type 1 (Th1) and 2 (Th2) cytokines, affecting the development and course of psoriasis. Until now, it is unclear whether traumatic stress experiences during the childhood or current stress are more frequent in psoriatic compared to skin-healthy individuals, and if they interact with treatment outcome.MethodIn a prospective cohort study, the impact of acute and early childhood stress on the course of dermatological treatment were studied in patients with moderate to severe psoriasis (PSO). Patients were examined before (T1) and about 3 months after (T2) the beginning of a new treatment episode. Assessments included clinical outcomes (Psoriasis Area and Severity Index—PASI, Structured Clinical Interview SCID-I) and patient-reported outcomes (PRO) (Childhood Trauma Questionnaire-CTQ, Perceived Stress Scale-PSS, itching/scratching, Dermatology Life Quality Index-DLQI, Hospital Anxiety and Depression Scale, Body Surface Area, Self-Administered PASI).ResultsN = 83 PSO patients (median age 53.7, IQR 37.8, 62.5) and n = 66 skin-healthy control subjects (HC) (median age 51.5, IQR 33.3, 59.2) participated. PSO had higher CTQ physical neglect than HC, as well as higher PRO levels. The positive impact of improved skin on the skin-related quality of life was moderated by the perceived stress. Acute stress at T1 had a positive effect both on the skin severity and the skin-related quality of life. CTQ total closely interacted with baseline psoriasis severity, and was associated with higher improvement from T1 to T2.ConclusionOne might tentatively conclude, that chronic psychosocial stressors like childhood maltreatment may predispose the manifestation of psoriasis. The latter may be amplified by acute psychological stressors. In addition, the present evidence suggests that systemic therapies work well in PSO, with childhood trauma and acute psychosocial stress. Both should therefore be routinely assessed and addressed in PSO.
Polish Version of the Post-traumatic Stress Disorder Related to COVID-19 Questionnaire COVID-19-PTSD
Authors: Kosydar-Bochenek Justyna , Krupa Sabina , Favieri Francesca , Forte Giuseppe , Medrzycka-Dabrowska Wioletta 
Published: April 2022

ObjectiveTranslate and investigate psychometric properties of the Polish version of COVID-19-PTSD in a sample of healthcare workers.MethodsThe PTSD symptoms were investigated among 184 participants (physicians, nurses, and paramedics). The respondents completed Post-Traumatic Stress Disorder Related to COVID-19 Questionnaire (COVID-19-PTSD) via online survey. The psychometric properties (i.e., internal consistency, validity, and reliability) of the Polish version of COVID-19-PTSD were analyzed.ResultsThe findings showed that the Polish version of COVID-19-PTSD is a reliable instrument. The total and subscale scores demonstrated good internal consistency. We also found that the prevalence of PTSD was reported at around 32% of healthcare workers.DiscussionThe Post-Traumatic Stress Disorder Related to COVID-19 Questionnaire (COVID-19-PTSD) is a first tool designed to assess the severity of PTSD symptoms related to the pandemic. The findings of our study confirmed good validity and reliability of the Polish version of COVID-19-PTSD which can be recommended to be used as a reliable screening tool to conduct psychological screening among Polish healthcare workers.
Importance of Linear Combination Modeling for Quantification of Glutathione and γ-Aminobutyric Acid Levels Using Hadamard-Edited Magnetic Resonance Spectroscopy
Authors: Song Yulu , Zöllner Helge J. , Hui Steve C. N. , Hupfeld Kathleen , Oeltzschner Georg , Prisciandaro James J. , Edden Richard 
Published: April 2022

BackgroundJ-difference-edited 1H-MR spectra require modeling to quantify signals of low-concentration metabolites. Two main approaches are used for this spectral modeling: simple peak fitting and linear combination modeling (LCM) with a simulated basis set. Recent consensus recommended LCM as the method of choice for the spectral analysis of edited data.PurposeThe aim of this study is to compare the performance of simple peak fitting and LCM in a test-retest dataset, hypothesizing that the more sophisticated LCM approach would improve quantification of Hadamard-edited data compared with simple peak fitting.MethodsA test–retest dataset was re-analyzed using Gannet (simple peak fitting) and Osprey (LCM). These data were obtained from the dorsal anterior cingulate cortex of twelve healthy volunteers, with TE = 80 ms for HERMES and TE = 120 ms for MEGA-PRESS of glutathione (GSH). Within-subject coefficients of variation (CVs) were calculated to quantify between-scan reproducibility of each metabolite estimate.ResultsThe reproducibility of HERMES GSH estimates was substantially improved using LCM compared to simple peak fitting, from a CV of 19.0–9.9%. For MEGA-PRESS GSH data, reproducibility was similar using LCM and simple peak fitting, with CVs of 7.3 and 8.8%. GABA + CVs from HERMES were 16.7 and 15.2%, respectively for the two models.ConclusionLCM with simulated basis functions substantially improved the reproducibility of GSH quantification for HERMES data.
Immunogenicity of Inactivated SARS-CoV-2 Vaccines in Patients With Rheumatoid Arthritis: A Case Series
Authors: Zhao Ting , Shen Jiayan , Zhu Youyang , Tian Xiaofang , Wen Guangfen , Wei Yuanyuan , Xu Bonan , Fu Chenyang , Xie Zhaohu , Xi Yujiang , Li Zhenmin , Peng Jiangyun , Wu Yang , Tang Xiaohu , Wan Chunping , Pan Lei , Li Zhaofu , Qin Dongdong 
Published: April 2022

ObjectivesAttenuated humoral response to mRNA SARS-CoV-2 vaccines has been reported in some patients with autoimmune disease, e.g., rheumatoid arthritis (RA). However, data of immune responses to inactivated SARS-CoV-2 vaccine in the RA population are still unknown. Herein, the safety and immunogenicity of inactivated SARS-CoV-2 vaccines in RA patients were analyzed.MethodsSeventy five RA patients and 26 healthy controls (HC) were respectively recruited from Yunnan Provincial Hospital of Traditional Chinese Medicine and the community in Kunming city. Neutralizing Antibody (NAb) Test ELISA kit was used to measure the percentage of inhibition. AKA (anti-keratin antibody) positivity was detected using indirect immunofluorescence. Rheumatoid factor (RF)-IgA was detected by ELISA. RF-IgG, RF-IgM, and anti-cyclic citrullinated peptide (CCP) antibodies were measured by chemiluminescence. ESR (erythrocyte sedimentation rate) was detected by ESR analyzer. C-RP (c-reactive protein) was detected by immunoturbidimetry. NEUT% (percentage of neutrophils) and LYMPH% (percentage of percentage) were calculated by a calculation method.ResultsCompared with the HC group, the percentage of inhibition was significantly lower in RA patients receiving two doses of vaccines. Vaccines-induced percentage of inhibition was the lowest in RA patients who had not been vaccinated. In total 80.77% of the HC group had a percentage of inhibition ≧20%, compared with 45.24% of vaccinated RA patients and 6.06% of unvaccinated RA patients. Spearman correlation analysis revealed that antibody responses to SARS-CoV-2 did not differ between RA patients according to their age and disease duration. Furthermore, the results showed that no correlation was found between the percentage of inhibition and indices for RA, including RF-IgA, IgG, IgM; anti-CCP antibody; ESR; C-RP; NEUT% and LYMPH%.ConclusionOur study showed inactivated vaccine-induced SARS-COV-2 antibody responses differ in RA patients and healthy subjects, emphasizing the importance of a third or fourth vaccination in RA patients.
Response to COVID-19 in the Central African Republic: Coping Strategies Combined With China’s Experience
Authors: Gou Qiheng , Zhu Fubin , Xie Keqi , Li Yiping , Xie Yuxin 
Published: April 2022

Objectives: The weak health system, domestic political unrest, poverty, and many other factors in the Central African Republic (CAR) have left the country underprepared for the COVID-19 pandemic, resulting in a greater health threat to the entire country. Rapid measures must therefore be taken to prevent the further spread of COVID-19.Methods: This work encompassed a review of relevant literature. We aim to analyze how far Chinese COVID measures can be transferred to the context of the CAR.Results: We argue that the measure that the CAR can learn from China’s success is the involvement of community workers and that greater investment in this model may be the optimal solution. Help from the international community is urgently needed.Conclusion: The CAR can benefit from China’s successful experience in fighting the epidemic, but the disparity in the combined power of the two countries does not allow for simple replication of China’s strategy.
Map of a Bending Problem for Self-Similar Beams into the Fractal Continuum Using the Euler–Bernoulli Principle
Authors: Samayoa Ochoa Didier , Damián Adame Lucero , Kryvko Andriy 
Published: April 2022

The bending of self-similar beams applying the Euler–Bernoulli principle is studied in this paper. A generalization of the standard Euler–Bernoulli beam equation in the FdH3 continuum using local fractional differential operators is obtained. The mapping of a bending problem for a self-similar beam into the corresponding problem for a fractal continuum is defined. Displacements, rotations, bending moments and shear forces as functions of fractal parameters of the beam are estimated, allowing the mechanical response for self-similar beams to be established. An example of the structural behavior of a cantilever beam with a load at the free end is considered to study the influence of fractality on the mechanical properties of beams.
Seroprevalence of Hepatitis B and Hepatitis C in Inpatients for Alcohol and Drug Addiction Treatment in the Psychiatry Clinic of a Private Hospital
Authors: MIZRAKÇI Serpil , GUVEN Mukerrem , ALKAN Sevil 
Published: April 2022

Aim: Hepatitis B (HBV) and hepatitis C virus (HCV) infections pose a serious health problem in our country as well as all over the world. The main transmission routes of these agents include parenteral contact with infected blood or body fluids (percutaneous injury), sexual transmission, and contact with blood and body fluids of infected people. Accordingly, people with drug abuse are an important risk group for these infections. The number of studies in this field in Turkish context is limited. In this study, we aimed to evaluate the seroprevalence of HBV and HCV in inpatients for alcohol and substance addiction treatment in a private hospital psychiatry clinic.
Materials-Methods: This retrospective study relied on the files of the patients who were hospitalized in the Alcohol and Substance Treatment Clinic in the sense that the results of HBsAg, anti-HBs, and anti-HCV parameters were evaluated retrospectively from the hospital automation system.
Results: Of the 568 patient included in the study, 154 (27.1%) were female, 414 (72.9%) were male, and the mean age was 35 (18-88). Findings revealed that 7 (1.23%) of the patients were positive for HBsAg, 229 (40.4%) for anti-HBs, and 41 (7.21%) for anti-HCV. There was no significant difference between HBsAg and Anti-HCV positivity rates by gender.
Conclusion: In our study, anti-HCV positivity was detected at a very high rate (7.21%). It can be concluded that the evaluation of these people in terms of test accuracy with HCV RNA as well as treating the infections detected in this group with effective new generation antivirals will also be beneficial with respect to reducing the number of people who can transmit the infection.