bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of Epithelial-Mesenchymal Transition Maintains Stemness in Human Amniotic Epithelial Cells
Authors: Takano Chika , Horie Masafumi , Taiko Isamu , Trinh Quang Duy , Kanemaru Kazunori , Komine-Aizawa Shihoko , Hayakawa Satoshi , Miki Toshio 
Published: August 2022

Abstract
Human amniotic epithelial cells (hAECs), which are a type of placental stem cell, express stem cell marker genes and are capable of differentiating into all three germ layers under appropriate culture conditions. hAECs are known to undergo TGF-β-dependent epithelial-mesenchymal transition (EMT); however, the impact of EMT on the stemness or differentiation of hAECs has not yet been determined. Here, we first confirmed that hAECs undergo EMT immediately after starting primary culture. Comprehensive transcriptome analysis using RNA-seq revealed that inhibition of TGF-β-dependent EMT maintained the expression of stemness-related genes, including NANOG and POU5F1, in hAECs. Moreover, the maintenance of stemness did not affect the nontumorigenic characteristics of hAECs. We showed for the first time that TGF-β-dependent EMT negatively affected the stemness of hAECs, providing novel insight into cellular processes of placental stem cells.
Graphical abstract
Applicability and usefulness of the Declaration of Helsinki for forensic research with human cadavers and remains
Authors: Scarpulla Valentina , Amadasi Alberto , Pelotti Susi , Ingravallo Francesca 
Published: August 2022

AbstractBodies of deceased persons and human remains and their specimens (i.e., organs, bones, tissues, or biological samples) are essential in forensic research but ad hoc worldwide-recognized ethical standards for their use are still lacking. Such standards are needed both to avoid possible unethical practices and to sustain research in the forensic field. Pending consensus within the forensic science community regarding this topic, with this article we aim to stimulate a debate as to the applicability and usefulness of the Declaration of Helsinki in the field of forensic research involving human cadavers and remains. Considering the fundamental differences compared to clinical research involving human beings and the different moral obligations involved, we focus on the risks, burdens, and benefits of research, ethics committee approval, and informed consent requirements. The Declaration of Helsinki framework allows forensic researchers to focus on substantial ethical principles promoting the consistency, integrity, and quality of research. Consensus regarding ethical standards and the adoption of national and supranational laws that clearly regulate the use of human cadavers and remains, including those from autopsies, continues to be of primary importance for the forensic science community.
Comparing parental distress and children’s difficulties between parents of children with rheumatic diseases and parents of healthy children in families facing the COVID-19 pandemic
Authors: Bramanti Sonia M. , Manippa Valerio , Babore Alessandra , Dilillo Anna , Marcellino Alessia , Martucci Vanessa , Mallardo Saverio , Isoldi Sara , Bloise Silvia , Sanseviero Mariateresa , Iorfida Donatella , De Luca Enrica , Trumello Carmen , D’Alleva Francesca , Ventriglia Flavia , Lubrano Riccardo , Del Giudice Emanuela 
Published: August 2022

Abstract
The COVID-19 pandemic could be a threat for the health status of children with a chronic condition. The present study aimed to explore parents’ and children’s psychological adjustment during the current pandemic, pursuing a triple objective: to compare the psychological adjustment of parents of children with pediatric rheumatic diseases (PRDs) and parents of healthy children; to analyze children’s psychological symptoms (emotional problems and hyperactivity) before and during the COVID-19 pandemic, and with or without a PRDs diagnosis; to explore the associations of children’s emotional problems and hyperactivity with parents’ psychological adjustment, parent–child interactions and belonging or not to families with PRDs. This cross-sectional study involved 56 parents of children with PRDs and 53 parents of healthy children. Self-report questionnaires about parents’ depression, anxiety, parenting stress, and children’s emotional symptoms and hyperactivity-inattention were administered. No differences were detected on psychological adjustment between parents of children with PRDs and parents of healthy children. Parents of children with PRDs reported statistically significant higher levels of children’s emotional problems and hyperactivity before the pandemic, compared to parents of healthy children; during COVID-19 pandemic, emotional symptoms increased for both groups, while hyperactivity-inattention symptoms increased only in the group of healthy children. Children’s emotional difficulties were associated with higher levels of parental anxiety, worse parent–child interaction and having PRDs; children’s hyperactivity symptoms were related to parent–child difficult interaction and higher levels of parental depression. Findings suggest the importance to target the children in relation to their parents, when approaching the psychological aspects of PRDs.
Podoplanin is Responsible for the Distinct Blood and Lymphatic Capillaries
Authors: Jeong Donghyun Paul , Hall Eva , Neu Erin , Hanjaya-Putra Donny 
Published: August 2022

Abstract
Introduction
Controlling the formation of blood and lymphatic vasculatures is crucial for engineered tissues. Although the lymphatic vessels originate from embryonic blood vessels, the two retain functional and physiological differences even as they develop in the vicinity of each other. This suggests that there is a previously unknown molecular mechanism by which blood (BECs) and lymphatic endothelial cells (LECs) recognize each other and coordinate to generate distinct capillary networks.

Methods
We utilized Matrigel and fibrin assays to determine how cord-like structures (CLS) can be controlled by altering LEC and BEC identity through podoplanin (PDPN) and folliculin (FLCN) expressions. We generated BECΔFLCN and LECΔPDPN, and observed cell migration to characterize loss lymphatic and blood characteristics due to respective knockouts.

Results
We observed that LECs and BECs form distinct CLS in Matrigel and fibrin gels despite being cultured in close proximity with each other. We confirmed that the LECs and BECs do not recognize each other through paracrine signaling, as proliferation and migration of both cells were unaffected by paracrine signals. On the other hand, we found PDPN to be the key surface protein that is responsible for LEC-BEC recognition, and LECs lacking PDPN became pseudo-BECs and vice versa. We also found that FLCN maintains BEC identity through downregulation of PDPN.

Conclusions
Overall, these observations reveal a new molecular pathway through which LECs and BECs form distinct CLS through physical contact by PDPN which in turn is regulated by FLCN, which has important implications toward designing functional engineered tissues.
Natural almost Hermitian structures on conformally foliated 4-dimensional Lie groups with minimal leaves
Authors: Andersdotter Svensson Emma , Gudmundsson Sigmundur 
Published: August 2022

AbstractLet (G, g) be a 4-dimensional Riemannian Lie group with a 2-dimensional left-invariant, conformal foliation $$\mathcal {F}$$
F
with minimal leaves. Let J be an almost Hermitian structure on G adapted to the foliation $$\mathcal {F}$$
F
. We classify such structures J which are almost Kähler $$(\mathcal {A}\mathcal {K})$$

(
A
K
)

, integrable $$(\mathcal {I})$$

(
I
)

or Kähler $$(\mathcal {K})$$

(
K
)

. Hereby we construct several new multi-dimensional examples in each class.
An efficient bi-objective approach for dynamic economic emission dispatch of renewable-integrated microgrids
Authors: Gholami Khalil , Abbasi Maysam , Azizivahed Ali , Li Li 
Published: August 2022

AbstractTo overcome the challenges of conventional power systems, such as increasing power demand, requirements of stability and reliability, and increasing integration of renewable energy sources, the concept of microgrids was introduced and is currently one of the most important solutions for solving the mentioned problems. Generally, microgrids have two operating modes, namely grid-connected and islanded modes. Based on the literature and its unique characteristics, the islanded mode is more challenging than the other one. In this paper, a new self-adaptive comprehensive differential evolution (SACDE) algorithm is proposed for solving economic load dispatch (ELD) and combined economic emission dispatch (CEED) problems, achieving optimal power consumption in isolated microgrids. Initially, SACDE is employed for solving the ELD problem as a single-objective function, meaning that the operational cost is just considered as the objective function, and thereby, the resources are scheduled accordingly. Then, a multi-objective platform based on SACDE is also proposed to solve the CEED problem. It means two objective functions, including operational cost and emission, are simultaneously optimized. For evaluating the performance of the proposed method, three different scenarios under various cases are considered. According to the results, when SACDE is employed to solve the single objective function (cost minimization) problem, it has better performance than other methods. In terms of the bi-objective scheme (cost and emission minimization), SACDE is significantly superior to the price penalty factor technique which is frequently used in previous studies.
Evaluation of the Transfer of Training for a Sexual Assault Resistance Program Enhanced with Sexuality Education
Authors: Jeffrey Nicole K. , Senn Charlene Y. , Hobden Karen L. , Barata Paula C. , McVey Gail L. , Radtke H. Lorraine , Eliasziw Misha 
Published: August 2022

Abstract
Introduction
The Enhanced Assess, Acknowledge, Act (EAAA) Sexual Assault (SA) Resistance Program is a theoretically sound, evidence-based program providing SA resistance education within a positive sexuality framework. It was shown to substantially reduce sexual assault victimization among university women who participate (Senn et al. in New England Journal of Medicine 372(24), 2326-2335, 2015). Staff training can either enhance or impede successful program scale-up and implementation. In this paper, we evaluate the transfer of training to implementation sites (i.e., postsecondary institutions) using a train-the-trainer model.

Methods
Using pre- and post-training surveys and post-training interviews conducted from 2016 to 2020 with 33 implementation staff members from multiple sites, we answered the following research questions:
1. Did the training meet its overall goal of preparing implementation staff?
2. What training components were perceived to contribute to training effectiveness and implementation staff preparedness?

Results
Results suggested that our model of training was effective. Competence, confidence, and knowledge and ability increased significantly after training, and most staff perceived the training to be highly useful and effective (especially for preparing them to address EAAA participant issues). Practice and feedback from trainers through active learning techniques were especially important. Although implementation staff reported being well prepared to deliver the training or program, they reported being less prepared for handling other implementation-related activities and issues (that the training was not necessarily designed to address in-depth).

Conclusions
Our findings suggest a need to enhance existing training on self-care and supporting program facilitators and for ongoing support and reminders from program purveyors to ensure that implementers are making use of existing resources. This study fills important gaps in the literature as few studies have examined the transfer of training for SA prevention programming.
An action framework for the participatory assessment of nature-based solutions in cities
Authors: van der Jagt Alexander P. N. , Buijs Arjen , Dobbs Cynnamon , van Lierop Martina , Pauleit Stephan , Randrup Thomas B. , Wild Tom 
Published: August 2022

AbstractImpact assessment is a key step in mainstreaming urban nature-based solutions (NBS). Yet, it remains unclear if and how assessment frameworks influence urban planning, design and management. We contend that the potential of current NBS assessment frameworks is not fully exploited due to: (1) limited contextualisation of monitoring and assessment to place-specific contexts and (2) the depoliticisation of co-production. To address this, we present a practical five-step action framework to guide inclusive participation across different stages of monitoring and assessment of urban NBS, including indicator selection. Unlike previous approaches, applying selection criteria at the level of individual indicators, we also use criteria at the aggregate level of the indicator set. We conclude that participatory assessment contributes to mainstreaming urban NBS for sustainable and just cities, provided data is contextualised to local decision-making contexts and the process is designed to amplify marginalised voices.
Piloting Uncontrolled DCD Organ Donation in the UK; Overview, Lessons and Future Steps
Authors: Wu Diana A. , Oniscu Gabriel C. 
Published: August 2022

Abstract
Purpose of Review
We explore how to develop Maastricht category I and II donation in the UK. We discuss lessons learned from previous UK pilots and define future steps in the journey to establishing a sustainable uDCD programme in the UK.

Recent Findings
The emergence of normothermic regional perfusion (NRP) as a successful strategy in cDCD donation with excellent clinical results creates the optimal platform for the development of a uDCD programme. Coordinated logistics with ambulance services and ED departments, embedded donor coordination in ED, public acceptance and wider discussion on acceptable peri-mortem interventions are key for future developments.

Summary
A uDCD programme in the UK is feasible. Despite an increase in public awareness and recent changes in legislation, there remain several challenges. Recent advances in perfusion and preservation and an established national retrieval infrastructure, create the premises for future sustainable developments.
Microbial Interplay in Skin and Chronic Wounds
Authors: Sachdeva Chandni , Satyamoorthy Kapaettu , Murali Thokur Sreepathy 
Published: August 2022

Abstract
Purpose of Review
Microbial infections in chronic wounds can often lead to lower-limb amputation, decrease in quality of life, and increase in mortality rate, and there is an unmet need to distinguish between pathogens and colonisers in these chronic wounds. Hence, identifying the composition of healthy skin microbiota, microbes associated with chronic wound and healing processes, and microbial interactions and host response in healing wounds vs. non-healing wounds can help us in formulating innovative individual-centric treatment protocols.

Recent Findings
This review highlights various metabolites and biomarkers produced by microbes that have been identified to modulate these interactions, particularly those involved in host–microbe and microbe–microbe communication. Further, considering that many skin commensals demonstrate contextual pathogenicity, we provide insights into promising initiatives in the wound microbiome research.

Summary
The skin microbiome is highly diverse and variable, and considering its importance remains to be a hotspot of medical investigations and research to enable us to prevent and treat skin disorders and chronic wound infections. This is especially relevant now considering that non-healing and chronic wounds are highly prevalent, generally affecting lower extremities as seen in diabetic foot ulcers, venous leg ulcers, and pressure ulcers. Pathogenic bacteria are purported to have a key role in deferring healing of wounds. However, the role of skin microflora in wound progression has been a subject of debate. In this review, we discuss biomarkers associated with chronic wound microenvironment along with the relevance of skin microflora and their metabolites in determining the chronicity of wounds.
Modelling cropland expansion and its drivers in Trans Nzoia County, Kenya
Authors: Kipkulei Harison Kiplagat , Bellingrath-Kimura Sonoko Dorothea , Lana Marcos , Ghazaryan Gohar , Boitt Mark , Sieber Stefan 
Published: August 2022

AbstractPopulation growth and increasing demand for agricultural production continue to drive global cropland expansions. These expansions lead to the overexploitation of fragile ecosystems, propagating land degradation, and the loss of natural diversity. This study aimed to identify the factors driving land use/land cover changes (LULCCs) and subsequent cropland expansion in Trans Nzoia County in Kenya. Landsat images were used to characterize the temporal LULCCs in 30 years and to derive cropland expansions using change detection. Logistic regression (LR), boosted regression trees (BRTs), and evidence belief functions (EBFs) were used to model the potential drivers of cropland expansion. The candidate variables included proximity and biophysical, climatic, and socioeconomic factors. The results showed that croplands replaced other natural land covers, expanding by 38% between 1990 and 2020. The expansion in croplands has been at the expense of forestland, wetland, and grassland losses, which declined in coverage by 33%, 71%, and 50%, respectively. All the models predicted elevation, proximity to rivers, and soil pH as the critical drivers of cropland expansion. Cropland expansions dominated areas bordering the Mt. Elgon forest and Cherangany hills ecosystems. The results further revealed that the logistic regression model achieved the highest accuracy, with an area under the curve (AUC) of 0.96. In contrast, EBF and the BRT models depicted AUC values of 0.86 and 0.77, respectively. The findings exemplify the relationships between different potential drivers of cropland expansion and contribute to developing appropriate strategies that balance food production and environmental conservation.
Investigation of spin rotators in CEPC at the Z-pole
Authors: Xia Wenhao , Duan Zhe , Gao Jie , Wang Yiwei 
Published: August 2022

AbstractPurposeLongitudinal polarization is an important design aspect of the future 100 km-scale Circular Electron Position Collider (CEPC). Spin rotators are needed in the CEPC collider rings to make the beam polarization along the longitudinal direction at the interaction points (IPs). This paper focuses on the design of spin rotators for CEPC at theZ-pole (45.6 GeV).MethodsThe design of spin rotators in the CEPC at theZ-pole is based on solenoid magnets and horizontal bending magnets sections. The coupling of transverse motion introduced by solenoids is compensated with quadrupole lenses. Adjustments have been made to the layout to implement the spin rotators into the collider rings.ResultsLongitudinal polarized beam can be achieved at the IPs with the spin rotators. High degree of polarization is attainable, while the effect of spin rotators on orbital motion is acceptable. The detailed simulation results will be presented.ConclusionA solenoid-based spin rotator configuration is designed and integrated into the CEPC collider ring lattice. According to the simulation results, the polarization requirements can be satisfied.
Fuzzy Pattern Tree Evolution Using Grammatical Evolution
Authors: Murphy Aidan , Ali Muhammad Sarmad , Mota Dias Douglas , Amaral Jorge , Naredo Enrique , Ryan Conor 
Published: August 2022

AbstractA novel approach to induce Fuzzy Pattern Trees using Grammatical Evolution is presented in this paper. This new method, called Fuzzy Grammatical Evolution, is applied to a set of benchmark classification problems. Experimental results show that Fuzzy Grammatical Evolution attains similar and oftentimes better results when compared with state-of-the-art Fuzzy Pattern Tree composing methods, namely Fuzzy Pattern Trees evolved using Cartesian Genetic Programming, on a set of benchmark problems. We show that, although Cartesian Genetic Programming produces smaller trees, Fuzzy Grammatical Evolution produces better performing trees. Fuzzy Grammatical Evolution also benefits from a reduction in the number of necessary user-selectable parameters, while Cartesian Genetic Programming requires the selection of three crucial graph parameters before each experiment. To address the issue of bloat, an additional version of Fuzzy Grammatical Evolution using parsimony pressure was tested. The experimental results show that Fuzzy Grammatical Evolution with this extension routinely finds smaller trees than those using Cartesian Genetic Programming without any compromise in performance. To improve the performance of Fuzzy Grammatical Evolution, various ensemble methods were investigated. Boosting was seen to find the best individuals on half the benchmarks investigated.
Management of industrial slurries with a chemical catalyst: generation of organic sustainable solution
Authors: Plaza Tanya , Scott Ian , Vega Ivan , Chavez Viviana , Parodi Jorge 
Published: August 2022

AbstractSlurries are a problem for the food-producing industry. They are comprised of solid and liquid wastes which must be precipitated and packed in polymers, and then disposed of in special landfills. A package of chemical substances (TCAS-CATA) has been developed to control the smell of this type of material. However, questions arose as to whether it would have any effect on nutrient bioavailability after eliminating the odors. To this end, slurries from animal processing plants were treated with TCAS-CATA in different conditions, and the parameters of this resulting solution were measured. Our results indicate that the catalyst can increase 100% nitrogenated compound concentration (400 mg/L of nitrites and 250 mg/L of Nitrate). This nutrient liberation depends on the slurry source, i.e., slurries with shells generate fewer nitrogenated compounds, but deliver more calcium to the solution. The solution also generates germination in some seed types and can stimulate development in some types of plants. Finally, the catalyst reduces odor 100% without reducing the capacity of the slurry to deliver nutrients. Our data suggest that TCAS-CATA may be catalyzing the bioavailability of the compound from the slurry without requiring microbiological processes, which will reduce odor and permit the use of this slurry as a biological remediation, similar to what is achieved in composting systems, but without the associated thermophilic processes. Future studies will allow for more knowledge of the mechanism and handling industrial material for agricultural processes.