bluuebunny/crossref_metadata_2025_split huggingface dataset:
Natural variation and improved genome annotation of the emerging biofuel crop field pennycress (Thlaspi arvense)
Authors: García Navarrete Tatiana , Arias Cintia , Mukundi Eric , Alonso Ana Paula , Grotewold Erich 
Published: April 2022

Abstract
The Brassicaceae family comprises more than 3,700 species with a diversity of phenotypic characteristics, including seed oil content and composition. Recently, the global interest in Thlaspi arvense L. (pennycress) has grown as the seed oil composition makes it a suitable source for biodiesel and aviation fuel production. However, many wild traits of this species need to be domesticated to make pennycress ideal for cultivation. Molecular breeding and engineering efforts require the availability of an accurate genome sequence of the species. Here, we describe pennycress genome annotation improvements, using a combination of long- and short-read transcriptome data obtained from RNA derived from embryos of 22 accessions, in addition to public genome and gene expression information. Our analysis identified 27,213 protein-coding genes, as well as on average 6,188 biallelic SNPs. In addition, we used the identified SNPs to evaluate the population structure of our accessions. The data from this analysis support that the accession Ames 32872, originally from Armenia, is highly divergent from the other accessions, while the accessions originating from Canada and the United States cluster together. When we evaluated the likely signatures of natural selection from alternative SNPs, we found 7 candidate genes under likely recent positive selection. These genes are enriched with functions related to amino acid metabolism and lipid biosynthesis and highlight possible future targets for crop improvement efforts in pennycress.
Prioritizing the target audience for a hearing awareness campaign in Australia using the TARPARE model
Authors: Alperstein Simon , Beach Elizabeth Francis 
Published: April 2022

Summary
Hearing loss affects one in six Australians, and it is estimated that around one-third of all hearing loss is preventable. Over the past decade, there have been repeated calls for the development of a national campaign in Australia, aimed at raising the public’s awareness of hearing health. We identified six target groups either at risk of developing hearing loss, vulnerable to poor outcomes as a result of untreated hearing loss, or lacking awareness of hearing health services, and applied the TARPARE model as a way of gathering insight into how a national hearing campaign might prioritize these different population segments. An online survey of prominent Australian academics, health practitioners and advocacy professionals with expertise in hearing health and/or public health promotion was created, through which their views on each of six potential target groups that had been identified as likely recipients of a potential hearing health campaign was sought. Young children and caregivers received the highest score based on survey responses and TARPARE criteria. A qualitative analysis of open-ended survey responses from the survey respondents revealed three key themes to consider as part of a potential campaign: Accessibility and availability of hearing services, Deciding on a preventative or treatment-focused approach, and The difficulty of changing behavior.
Distal arch replacement for aortic aneurysm associated with pseudocoarctation through the L-incision approach
Authors: Goto Takuya , Koizumi Junichi , Saiki Hirofumi , Kin Hajime 
Published: April 2022

Abstract
We report the case of a 16-year-old boy in whom we successfully repaired a distal aortic arch aneurysm associated with pseudocoarctation using double aortic cannulation and antegrade selective cerebral perfusion through the L-incision approach. This approach provided excellent exposure from the ascending aorta to the descending aorta, which enabled total body perfusion. We avoided cardiac arrest and hypothermic circulatory arrest during the surgery. The L-incision approach could be a better alternative for aortic arch surgery in adolescents.
Robotic subxiphoid-optical thymectomy
Authors: Shimomura Masanori , Ishihara Shunta , Okada Satoru , Inoue Masayoshi 
Published: April 2022

Abstract
Robot-assisted thymectomy through a subxiphoid scopic approach can provide a good surgical view, similar to that of median sternotomy. We originally used the subxiphoid port only for the robotic scope to avoid instrument collision with the assistant device. This approach, robotic subxiphoid-optical thymectomy, is advantageous for the safe and accurate dissection of the bilateral phrenic nerves and the left brachiocephalic vein, which are especially needed in extended thymectomy for patients with myasthenia gravis.
Asking about Social Circles Improves Election Predictions Even with Many Political Parties
Authors: Bruine de Bruin Wändi , Galesic Mirta , Bååth Rasmus , de Bresser Jochem , Hall Lars , Johansson Petter , Strandberg Thomas , van Soest Arthur 
Published: April 2022

Abstract
Traditionally, election polls have asked for participants’ own voting intentions. In four elections, we previously found that we could improve predictions by asking participants how they thought their social circles would vote. A potential concern is that the social-circle question might predict results less well in elections with larger numbers of political options because it becomes harder to accurately track how social contacts plan to vote. However, we now find that the social-circle question performs better than the own-intention question in predicting two elections with many political parties: The Netherlands’ 2017 general election and the Swedish 2018 general election.
The energy requirement for maintenance of Nellore crossbreds in tropical conditions during the finishing period
Authors: Goulart Rodrigo S , Tedeschi Luis O , Silva Saulo L , Leme Paulo R , de Alencar Maurício M , Lanna Dante P D 
Published: April 2022

Abstract
This study determined the energy requirement for maintenance of purebred Nellore cattle and its crossbreds using data from a comparative slaughter trial in which animals were raised under the same plane of nutrition from birth through slaughter and born from a single commercial Nellore cowherd. A total of 79 castrated steers (361 ± 54 kg initial body weight [BW]) were used in a completely randomized design by age (22 mo ± 23 d of age) with four genetic groups (GG): Nellore (NL), ½ Angus × ½ Nellore (AN), ½ Canchim × ½ Nellore (CN), and ½ Simmental × ½ Nellore (SN). The experimental design provided ranges in metabolizable energy (ME) intake (MEI), BW, and average daily gain needed to develop regression equations to predict net energy for maintenance (NEm) requirements. Four steers of each GG were slaughtered to determine the initial body composition. The remaining 63 steers were assigned to different nutritional treatments (NT) by GG; ad libitum or limit-fed treatments (receiving 70% of the daily feed of the ad libitum treatment of the same GG). Full BW was recorded at birth, weaning, 12, 18, and 22 mo. In the feedlot, steers were fed for 101 d a diet containing (DM basis) 60% corn silage and 40% concentrate. No difference in age at weaning (P = 0.534) and slaughter (P = 0.179 and P = 0.896, for GG and NT, respectively) were observed. AN steers were heavier at weaning weight, yearling weight and had higher empty BW (EBW; P = 0.007, P = 0.014, and P &lt; 0.001, respectively) in comparison to NL, CN, and SN. There were no interactions (P &gt; 0.05) between GG and NT for any variable evaluated. When fed ad libitum, AN steers had higher daily MEI (Mcal/d; P &lt; 0.001) in comparison to NL, CN, and SN. On a constant age basis, differences were observed on body composition (P &lt; 0.05) between GG. The slope (P = 0.600) and intercept (P = 0.702) of the regression of log heat production on MEI were similar among GG. Evaluating at the same age and the same frame size, there were no differences in NEm requirement between Nellore and AN (P = 0.528), CN (P = 0.671), and SN (P = 0.706). The combined data indicated a NEm requirement of 86.8 kcal/d/kg0.75 EBW and a ME required for maintenance requirement had a common value of 137.53 kcal/d/kg0.75 EBW. The efficiency of energy utilization for maintenance and the efficiency of energy utilization for growth values were similar among GG (P &gt; 0.05 and P &gt; 0.05, respectively) and were on average 63.2% and 26.0%, respectively. However, although not statistically different, the NEm values from NL showed a decrease in NEm of 5.76% compared with AN steers.
Elliptical and linear relationships with rumen temperature support a homeorhetic trajectory for DMI during recovery of feedlot cattle exposed to moderate heat load
Authors: Sullivan Megan L , Wijffels Gene , George A , Al-Hosni Yousef A , Olm Joseph C W , Gaughan John B 
Published: April 2022

Abstract
Most feedlot animals in Australia experience 2 to 3 moderate heat waves during summer. This study aimed to gain understanding of the physiological drivers in response to and during recovery from such events with a view to designing strategies to ensure rapid and safe recovery. Two hypotheses were tested during thermal challenge and recovery in climate-controlled rooms (CCR): firstly, the feedlot steer on a grain-based diet mounts appropriate physiological responses during moderate heat load and in recovery so that its performance and physiology state after recovery is not different to the feed restricted thermoneutral (FRTN) steer. Secondly, commonly used indicators of increased heat load, e.g., respiration rate (RR), panting score (PS), body surface temperatures (ST), and water consumption (WC), reflect rumen temperature (RT) during thermal challenge and recovery at the level of daily means. In this study, 36 Angus steers (live weight (LW) 451.5 ± 22.6 kg) made up 3 cohorts of 12 animals that sequentially underwent the CCR phase. For this 18-d phase, the steers were allocated to either a moderate heat load treatment (thermally challenged, TC, n = 18) or a FRTN treatment (n = 18). The TC group underwent 3 periods, Pre-Challenge (4 d, temperature humidity index (THI) range of 68 to 71), Challenge (7 d, THI 73 to 84 with diurnal cycling), and Recovery (7 d, THI 68 to 71). The FRTN group were held at thermoneutral conditions in the CCR (THI 66.9 ± 0.3), and each animal was offered an amount of feed was based on the feed intake of its LW matched TC pair. Thus, as DMI fell in the TC group during Challenge, feed restriction was imposed on the FRTN group. The data were collected by trained observers were DMI, RT, RR, PS, body STs (forehead, shoulder, leg, rump), and WC. Challenge induced a heat stress response in the TC group with reduced DMI and LW, and elevated RT, RR, PS, body STs, and WC (P &lt; 0.001). These measures were unchanged or reduced in the FRTN group (P &lt; 0.001). At the end of Recovery, the TC and FRTN groups had converged on most measures including LW. Daily mean RT of both groups showed strong linear relationships with THI, RR, PS, head ST, and WC (P ≤ 0.0022) but opposing elliptical relationships with DMI; that is, as DMI fell with increasing RT for the TC group, DMI increased with rising RT for the FRTN group. In all, the feedlot steers in this study demonstrated sufficient homeorhetic capacity to adjust to moderate heat load and recover from it.
Investigation of serum markers of esophageal squamous cell carcinoma based on machine learning methods
Authors: Ma Zhifeng , Zhu Ting , Wang Haiyong , Wang Bin , Fu Linhai , Yu Guangmao 
Published: April 2022

Abstract
Esophageal squamous cell carcinoma (ESCC) is one of the malignant tumors with high mortality in humans, and there is a lack of effective and convenient early diagnosis methods. By analyzing the serum miRNA expression data in ESCC tumor samples and normal samples, on the basis of the maximal relevance and minimal redundancy (mRMR) feature selection and the incremental feature selection method, a random forest classifier constructed by five-feature miRNAs was acquired in our study. The receiver operator characteristic curve showed that the model was able to distinguish samples. Principal component analysis (PCA) and sample hierarchical cluster analysis showed that five-feature miRNAs could well distinguish ESCC patients from healthy individuals. The expression levels of miR-663a, miR-5100 and miR-221-3p all showed a higher expression level in ESCC patients than those in healthy individuals. On the contrary, miR-6763-5p and miR-7111-5p both showed lower expression levels in ESCC patients than those in healthy individuals. In addition, the collected clinical serum samples were used for qRT-PCR analysis. It was uncovered that the expression trends of the five-feature miRNAs followed a similar pattern with those in the training set. The above findings indicated that the five-feature miRNAs may be serum tumor markers of ESCC. This study offers new insights for the early diagnosis of ESCC.
Detecting SARS-CoV-2 neutralizing immunity: highlighting the potential of split nanoluciferase technology
Authors: Jeremiah Sundararaj Stanleyraj , Miyakawa Kei , Ryo Akihide 
Published: April 2022

Abstract
The coronavirus disease 2019 (COVID-19) pandemic has progressed over 2 years since its onset causing significant health concerns all over the world and is currently curtailed by mass vaccination. Immunity acquired against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be following either infection or vaccination. However, one can never be sure whether the acquired immunity is adequate to protect the individual from subsequent infection because of three important factors: individual variations in humoral response dynamics, waning of protective antibodies over time, and the emergence of immune escape mutants. Therefore, a test that can accurately differentiate the protected from the vulnerable is the need of the hour. The plaque reduction neutralization assay is the conventional gold standard test for estimating the titers of neutralizing antibodies that confer protection. However, it has got several drawbacks, which hinder the practical application of this test for wide-scale usage. Hence, various tests have been developed to detect protective immunity against SARS-CoV-2 that directly or indirectly assess the presence of neutralizing antibodies to SARS-CoV-2 in a lower biosafety setting. In this review, the pros and cons of the currently available assays are elaborated in detail and special focus is put on the scope of the novel split nanoluciferase technology for detecting SARS-CoV-2 neutralizing antibodies.
Risk of Metabolic and Cardiovascular Adverse Events With Abiraterone or Enzalutamide Among Men With Advanced Prostate Cancer
Authors: Lai Lillian Y , Oerline Mary K , Caram Megan E V , Tsao Phoebe A , Kaufman Samuel R , Hollenbeck Brent K , Shahinian Vahakn B 
Published: April 2022

Abstract

Background
Abiraterone and enzalutamide are the most common oral agents for the treatment of men with advanced prostate cancer. To understand their safety profiles in real-world settings, we examined the association between the use of abiraterone or enzalutamide and the risk of metabolic or cardiovascular adverse events while on treatment.

Methods
Men with advanced prostate cancer and their use of abiraterone or enzalutamide were identified in a 20% sample of the 2010-2017 national Medicare claims. The primary composite outcome was the occurrence of a major metabolic or cardiovascular adverse event, defined as an emergency room visit or hospitalization associated with a primary diagnosis of diabetes, hypertension, or cardiovascular disease. The secondary composite outcome was the occurrence of a minor metabolic or cardiovascular adverse event, defined as an outpatient visit associated with a primary diagnosis of the aforementioned conditions. Risks were assessed separately for abiraterone and enzalutamide using Cox regression. All statistical tests were 2-sided.

Results
Compared with men not receiving abiraterone, men receiving abiraterone were at increased risk of both a major composite adverse event (hazard ratio [HR] = 1.77, 95% confidence interval [CI] = 1.53 to 2.05; P &lt; .001) and a minor composite adverse event (HR = 1.24, 95% CI = 1.05 to 1.47; P = .01). Compared with men not receiving enzalutamide, men receiving enzalutamide were at an increased risk of a major composite adverse event (HR = 1.22, 95% CI = 1.01 to 1.48; P = .04) but not a minor composite adverse event (HR = 1.04, 95% CI = 0.83 to 1.30; P = .75).

Conclusion
Careful monitoring and management of men on abiraterone or enzalutamide through team-based approaches are critical.
Resourcing Goal-directed Networks: Toward A Practice-based Perspective
Authors: Auschra Carolin , Sydow Jörg 
Published: April 2022

AbstractThis paper proposes a practice-based perspective on how managers resource goal-directed networks in the public sector, especially those governed by a network administrative organization. While previous literature shows that network managers need to acquire and allocate resources in order to achieve network goals, little is known about specific resourcing practices and related challenges to resourcing goal-directed networks. To shed light on these issues, we outline a processual, multilevel, network-centric perspective that focuses on network resourcing practices and takes their interplay with network rules and goals into account. This paper shows that, to attain network goals, network managers need to mitigate developing tensions arising from the different interests of network members, external stakeholders, and the network itself, while navigating a trajectory of network resourcing. The paper contributes to the literature on public networks by examining potential sources of network-level resources; outlining basic resourcing practices of controlling, producing, reproducing, and transforming such resources; discussing multilevel tensions around network resourcing; and exploring trajectories of network resourcing. In addition, we propose avenues for empirical research on network resourcing.
Effects of tree mycorrhizal type on soil respiration and carbon stock via fine root biomass and litter dynamic in tropical plantations
Authors: Zhang Guodong , Zhou Guiyao , Zhou Xuhui , Zhou Lingyan , Shao Junjiong , Liu Ruiqiang , Gao Jing , He Yanghui , Du Zhenggang , Tang Jianwei , Delgado-Baquerizo Manuel 
Published: April 2022

Abstract
Tropical forests are among the most productive and vulnerable ecosystems in the planet. Several global forestation programs are aiming to plant millions of trees in tropical regions in the future decade. Mycorrhizal associations are known to largely influence forest soil carbon (C) stocks. However, to date, little is known on whether and how different tree mycorrhizal types affect soil respiration (Rs) and C stocks in tropical forests. In this study, we used a three-decade tropical common garden experiment, with three arbuscular mycorrhizal (AM) and three ectomycorrhizal (EM) monocultures, to investigate the impacts of tree mycorrhizal type on Rs and soil C stocks. Associating biotic (e.g. root biomass, litter dynamic, soil microbes) and abiotic factors (e.g. microclimate) were also measured. Our results showed that AM stands supported significantly higher Rs and soil C stock, litter turnover rate and fine root biomass than EM stands. Further statistical analysis displayed that tree mycorrhizal type was the most important factor in regulating Rs and soil C stock compared with other biotic or abiotic factors. Moreover, we found that mycorrhizal type directly and indirectly affected Rs and soil C stocks via fine root biomass and litter dynamic, i.e. litter production, litter standing crop and litter turnover rate. Our findings highlight important effects of tree mycorrhizal type on forest C cycle, suggesting that planting AM tree species could contribute to promotion of soil C stock in tropical ecosystems.
Energetic transients joint analysis system for multi-INstrument (ETJASMIN) for GECAM – I. Positional, temporal, and spectral analyses
Authors: Xiao Shuo , Xiong Shao-Lin , Cai Ce , Song Li-Ming , Zheng Shi-Jie , Peng Wen-Xi , Wang Ping , Qiao Rui , Guo Dong-Ya , Wang Jin , Li Xiao-Bo , Song Xin-Ying , Yuan Yong , Fan Xi-Long , Zhao Xiao-Yun , Huang Yue , Ma Xiang , Zhang Peng , Li Bing , Ge Ming-Yu , Tuo You-Li , Chen Wei , Zhang Hong-Mei , He Jian-Jian , Li Chao-Yang , Yi Qi-Bin , Zhao Yi , Zhang Yan-Qiu , Zheng Chao , Xue Wang-Chen , Liu Jia-Cong , Zhang Zhen , Li Cheng-Kui , Zhang Xiao-Lu , Zhao Hong-Yu , Zhao Guo-Ying , Guo Zhi-Wei , Xie Sheng-Lun , Wang Chen-Wei , Zhang Bo-Xin , Wang Yue , Li Qing-Xin , Li Chao , Zhang Kai , Shi Dong-Li , Zhao Shi-Yi , Yao Min , An Zheng-Hua , Chen Chan , Gong Ke , Liu Ya-Qing , Gao Min , Li Xin-Qiao , Li Yan-Guo , Liang Xiao-Hua , Liu Xiao-Jing , Sun Xi-Lei , Wang Jin-Zhou , Wen Xiang-Yang , Xu Yan-Bing , Xu Yu-Peng , Yang Sheng , Zhang Chao-Yue , Zhang Da-Li , Zhang Fei , Chen Gang , Lu Fang-Jun , Sun Gong-Xing , Zhang Fan , Zhang Shuang-Nan 
Published: April 2022

ABSTRACT
Gravitational wave high-energy Electromagnetic Counterpart All-sky Monitor (GECAM) is a dedicated mission, launched in December 2020, for gamma-ray transients, including gamma-ray bursts (GRBs) and soft gamma repeater (SGR) bursts in the multimessenger and multiwavelength astronomy era. Since GECAM consists of two independent spacecrafts (or say instruments), and the framework of data analysis for multiple spacecrafts is distinctive from that for only one spacecraft, which is the case for most GRB missions, we developed a dedicated pipeline called Energetic Transients Joint Analysis System for Multi-INstrument (ETJASMIN) for GECAM mission. This pipeline has been naturally extended to incorporate data from other gamma-ray instruments, including the operating missions, such as Insight-HXMT/HE, Fermi/GBM, Swift/BAT, INTEGRAL/SPI-ACS, Konus-Wind, and GRID, as well as the forthcoming missions, such as SVOM/GRM and HEBS. In this paper, we present this pipeline with a focus on the data analysis procedures, methodology, and results in terms of the localization, verification (classification), spectral, and temporal analyses of gamma-ray transients. We show that this pipeline could provide more accurate, reliable, and comprehensive results than that of individual spacecraft, which is beneficial for gamma-ray transients observation.
Glioblastoma scRNA-seq shows treatment-induced, immune-dependent increase in mesenchymal cancer cells and structural variants in distal neural stem cells
Authors: Couturier Charles P , Nadaf Javad , Li Zhaorong , Baig Salma , Riva Gabriele , Le Phuong , Kloosterman Daan J , Monlong Jean , Nkili Meyong Andriniaina , Allache Redouane , Degenhard Theresa , Al-Rashid Mariam , Guiot Marie-Christine , Bourque Guillaume , Ragoussis Jiannis , Akkari Leila , Quintana Francisco J , Petrecca Kevin 
Published: April 2022

Abstract

Background
Glioblastoma is a treatment-resistant brain cancer. Its hierarchical cellular nature and its tumor microenvironment (TME) before, during, and after treatments remain unresolved.

Methods
Here, we used single-cell RNA sequencing to analyze new and recurrent glioblastoma and the nearby subventricular zone (SVZ).

Results
We found 4 glioblastoma neural lineages are present in new and recurrent glioblastoma with an enrichment of the cancer mesenchymal lineage, immune cells, and reactive astrocytes in early recurrences. Cancer lineages were hierarchically organized around cycling oligodendrocytic and astrocytic progenitors that are transcriptomically similar but distinct to SVZ neural stem cells (NSCs). Furthermore, NSCs from the SVZ of patients with glioblastoma harbored glioblastoma chromosomal anomalies. Lastly, mesenchymal cancer cells and TME reactive astrocytes shared similar gene signatures which were induced by radiotherapy in a myeloid-dependent fashion in vivo.

Conclusion
These data reveal the dynamic, immune-dependent nature of glioblastoma’s response to treatments and identify distant NSCs as likely cells of origin.