bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐term impact of patients' compliance to peri‐implant maintenance therapy on the incidence of peri‐implant diseases: An 11‐year prospective follow‐up clinical study
Authors: Costa Fernando Oliveira , Costa Adriana Moreira , Ferreira Sérgio Diniz , Lima Rafael Paschoal Esteves , Pereira Gustavo Henrique Mattos , Cyrino Renata Magalhães , Oliveira Alcione Maria Soares Dutra , Oliveira Peterson Antônio Dutra , Cota Luís Otávio Miranda 
Published: December 2022

AbstractObjectiveTo prospectively evaluate the incidence of peri‐implant diseases and the associated risk factors during 11 years of peri‐implant maintenance therapy (PIMT).Material and MethodsA sample of 80 partially edentulous individuals rehabilitated with dental implants was invited to participate in a PIMT program (T1—prior to entering the PIMT program). After 11 years, 51 individuals remained regularly or irregularly adherent to PIMT (T2—last recall after 11 years) and were classified as regular (RC; n = 27) or irregular (IC; n = 24) compliers. Data of interest were analyzed using univariate and multivariate logistic regression analyses.ResultsThe incidence of peri‐implant mucositis and peri‐implantitis observed at T2 in the IC group (70.8% and 37.5%, respectively) were significantly higher than those observed in the RC group (37.0% and 11.1%, respectively). The incidence of peri‐implant diseases was mostly attributable to potentially modifiable risk factors, as such: RC group—PM (p = 0.013); IC group—high plaque index (p < 0.001), irregular compliance (p < 0.001), the presence of PM (p = 0.015) and periodontitis (p < 0.039).ConclusionRegular compliance during PIMT had a strong effect in minimizing the incidence of peri‐implant diseases. Increasing regular dental visits and improving oral hygiene would provide benefits for preventing peri‐implant diseases.
Visiting ‘home’: Considering diasporic practices through assemblage dynamics
Authors: Wagner Lauren B 
Published: December 2022

AbstractVisiting ‘home’ as a migrant may not always be about going home. Exploring a case where visiting is motivated by tourism as much as – or more than – migration, I argue for using assemblage as a set of ontological premises enables alternative appreciations of how practices of ‘visiting home’ evolve. Starting from a primacy of relationality and of malleable materialities, this perspective does not rely on migration‐defined polarities to frame the spectrum of belonging in a homeland but allows for influences from many sources to interact and generate new formations that exceed the sum of their parts. Within this case, I analyse diasporic practices of visiting through three entwined dynamics: a contradictory sense of attachment to a place of ancestral origin, a desire for embodied leisure on vacation, and an instinct to insulate oneself from certain others. All three simultaneously contribute to the potency and perpetuation of diasporic visiting in Morocco.
circTMEM181 upregulates ARHGAP29 to inhibit hepatocellular carcinoma migration and invasion by sponging miR‐519a‐5p
Authors: Chen Bing , Li Qiaojuan , Li Yuchen , Li Qin , Lai Hongyan , Huang Shenglin , Li Caiping , Li Yan 
Published: December 2022

AbstractAimCircular RNAs (circRNAs) are a novel class of noncoding RNAs and are conserved in various species. Although numerous circRNAs have been identified, their role in cancer remains unclear.MethodsThe expression of circTMEM181 in 90 paired human hepatocellular carcinoma (HCC) and adjacent nontumor tissues were detected using quantitative reverse transcription–polymerase chain reaction. Transwell assay was performed for functional analysis of HCC cell migration and invasion. Luciferase reporter assay was used to verify the combination of circTMEM181 and miR‐519a‐5p.ResultsIn this study, we identified a novel circRNA, named circTMEM181, was downregulated in HCC tissues. Decreased expression of circTMEM181 was associated with shorter overall survival of patients with HCC. CircTMEM181 overexpression reduced HCC cell migration and invasion abilities, while circTMEM181 knockdown increased cell motility. Mechanically, circTMEM181 could directly bind to miR‐519a‐5p and subsequently upregulate ARHGAP29 protein expression.ConclusionThese data provide the first evidence of clinical significance and function of circTMEM181, and suggest the circTMEM181/miR‐519a‐5p/ARHGAP29 axis in HCC development.
Trends in school‐neighbourhood inequalities and youth obesity: Repeated cross‐sectional analyses of the public schools in the state of California
Authors: Matsuzaki Mika , Sanchez‐Vaznaugh Emma V. , Alexovitz Kelsey , Acosta Maria E. , Sánchez Brisa N. 
Published: December 2022

SummaryBackgroundIt is currently unknown whether the relationship between affluence of school neighbourhoods and prevalence of youth overweight/obesity is uniform across demographic subgroups and areal context in the United States.MethodsWe examined association between school‐neighbourhood income tertiles and school‐level overweight/obesity (OVOB) prevalence, using data on body mass index of fifth, seventh, and nineth graders who attended public schools in California in 2001 and 2010 (n = 1 584 768), using multiple logistic regression models.ResultsOverall, OVOB prevalence was higher in lower‐income school neighbourhoods, with a steeper income‐OVOB gradient for girls. Among boys, the gradient became steeper in 2010 than 2000. Among Asian and White girls, the negative gradients were steepest in rural areas. For African–American students in all areas and Latino boys in rural areas, there was less clear evidence of inverse income‐OVOB gradients. Addition of fast‐food restaurant availability to the models did not change the observed inverse school‐neighbourhood income–obesity gradients.ConclusionsThe findings suggest the needs to investigate reasons for this variability with consideration to combinations of sociodemographic, economic, and environmental risk factors that may contribute to disparities in childhood obesity.
Trends of radical cystectomy and comparisons of surgical outcomes among surgical approaches focusing on robot‐assisted radical cystectomy: A Japanese nationwide database study
Authors: Chen Wei , Yokoyama Minato , Kobayashi Masaki , Fan Bo , Fukuda Shohei , Waseda Yuma , Tanaka Hajime , Yoshida Soichiro , Ai Masumi , Fushimi Kiyohide , Nonomura Norio , Fujii Yasuhisa 
Published: December 2022

ObjectiveTo clarify the trends in radical cystectomy and to compare surgical outcomes among surgical approaches focusing on robot‐assisted radical cystectomy based on a Japanese nationwide database.MethodsThe Diagnosis Procedure Combination database was used to extract data on radical cystectomy cases. Trends in open radical cystectomy, laparoscopic radical cystectomy, minimum incision endoscopic radical cystectomy, and robot‐assisted radical cystectomy between April 2012 and March 2021 were evaluated. Basic characteristics and peri‐operative indicators were compared among the four groups. Propensity score matching was applied to assess the differences between open radical cystectomy and robot‐assisted radical cystectomy.ResultsDuring the study period, a decreasing number of open radical cystectomies and an increasing number of minimally invasive radical cystectomies were shown in the total cohort of 28 345 cases. The number of robot‐assisted radical cystectomies rapidly increased after government approval in 2018. Minimally invasive radical cystectomies, including robot‐assisted radical cystectomies, had a significantly lower complication rate, a shorter length of stay, and a lower blood transfusion rate, but a longer anesthesia time than open radical cystectomies. In the propensity score matching analysis comparing the surgical outcomes of robot‐assisted radical cystectomy and open radical cystectomy, similar results were demonstrated, and blood transfusion rates were equivalent.ConclusionFor the past decade, the number of minimally invasive radical cystectomies has steadily increased without compromised surgical outcomes, except for anesthesia time. Robot‐assisted radical cystectomies in particular rapidly became widespread in Japan after government approval in 2018 and became a standard surgery within the first 3 years.
Clinical outcomes of negative pressure wound therapy with instillation vs standard negative pressure wound therapy for wounds: A meta‐analysis of randomised controlled trials
Authors: Wang Guoqi , Xu Haiyan , Xu Gaoxiang , Zhang Hao , Li Zhirui , Liu Daohong 
Published: December 2022

AbstractNegative pressure wound therapy (NPWT) with or without instillation has been extensively applied for patients with multiple wound types. Whether NPWT with instillation is superior to NPWT alone is not known. This study aims to compare the efficacy between negative pressure wound therapy with instillation (NPWTi) and standard negative pressure wound therapy for wounds. The authors searched for randomised controlled trials (RCTs) in PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials investigating clinical outcomes of negative pressure wound therapy with instillation vs standard negative pressure wound therapy for wounds. The registration number (protocol) on PROSPERO is CRD42022287178. Eight RCTs involved 564 patients met the inclusion criteria and were included finally. NPWTi showed a significant fewer surgeries and dressing changes (RR and 95% CI, −9.31 [−17.54, −1.08], P < 0.05), and smaller wound area after treatment (RR and 95% CI, −9.31 [−17.54, −1.08], P < 0.05) compared with NPWT. No significant difference was observed on healing rate, time to heal, length of stay, dehiscence, reinfection, reoperation and readmission between NPWTi and NPWT. The addition of instillation to NPWT could improve clinical outcomes regarding the number of surgeries and dressing changes, and wound area after treatment in patients with multiple wound types. However, because of the heterogeneity these conclusions still need to be further validated by more well‐designed RCTs with large sample sizes.
Bioinformatics analysis and identification of dysregulated POSTN in the pathogenesis of keloid
Authors: Xu Hailin , Wang Zhiyong , Yang Hao , Zhu Jiayuan , Hu Zhicheng 
Published: December 2022

AbstractKeloid is a benign fibro‐proliferative dermal tumour formed by an abnormal scarring response to injury and characterised by excessive collagen accumulation and invasive growth. The pathophysiology of keloids is complex, and the treatment for keloids is still an unmet medical need. Here, we investigated the transcriptional gene that influences keloid development by comparing keloid, non‐lesioned keloid skin and normal skin as well as keloid fibroblast and normal fibroblast (GSE83286, GSE92566, GSE44270). Based on the analysis, 146 up‐regulated genes and 48 down‐regulated genes were found in keloid tissue compared with normal skin and keloid no‐lesioned skin. Eleven genes were further identified by overlapping the DEGs from keloid tissue described previously with DEGs in keloid fibroblast. The overlapped genes included PRR16, SFRP2, EDIL3, GERM1, POSTN, PDE3A, GALNT5, F2RL2, EYA4, ZFHX4, and AIM2. POSTN is the most crucial node in PPI network, which mainly correlate to collagen‐related genes. Moreover, siRNA knockdown identified POSTN is a crucial regulatory gene that regulates keloid fibroblast migration and collagen I, collagen III expression level. In conclusion, our study identified 11 hub genes that play crucial role in keloid formation and provided insights for POSTN to be the therapeutic target for keloid through bioinformatic analysis of three datasets. Additionally, our results would support the development of future therapeutic strategies.
Optimized weighted mobility induced high thermoelectric performance of ZnO‐based multilayered thin films
Authors: Zhou Zhifang , Zheng Yunpeng , Yang Yueyang , Zhang Wenyu , Wei Bin , Zou Mingchu , Lan Jinle , Nan Ce‐Wen , Lin Yuan‐Hua 
Published: December 2022

AbstractAs a transparent thermoelectric oxide, gallium‐doped zinc oxide (GZO) has the potential to power wearable or portable electronics and may be used in the integrated circuits industry for chip cooling. Constructing ZnO–GZO interfaces has been proposed as an effective strategy for improving thermoelectric performance of GZO thin films. However, without the aid of band structure calculation for multilayered films, it is hard to directly elucidate the underlying mechanisms of carrier transport. Weighted mobility is an indicator that reveals the inherent electronic transport properties like carrier scattering, electronic band structure, and so on. Thus, to further investigate the effects of ZnO–GZO interfaces on electrical properties of GZO thin films, the structures containing different numbers of ZnO–GZO interfaces were designed and the correlations among numbers of ZnO–GZO interfaces, weighted mobility, and electrical properties were explored. It was found that with more ZnO–GZO interfaces, the weighted mobility increased, and the power factor values also improved as well. Consequently, an enhanced power factor value reached 439 μW m−1 K−2 at 623 K. This work demonstrated the beneficial effects of multiple interfaces on the improvements of electrical transport performance through analyzing weighted mobility, which laid a foundation for further optimization of thermoelectric performance.
ANAC087 transcription factor positively regulates age‐dependent leaf senescence through modulating the expression of multiple target genes in Arabidopsis
Authors: Chen Qinqin , Yan Jingli , Tong Tiantian , Zhao Peiyu , Wang Shuangshuang , Zhou Na , Cui Xing , Dai Moyu , Jiang Yuan‐Qing , Yang Bo 
Published: December 2022

AbstractLeaf senescence is the final stage of leaf development and appropriate onset and progression of leaf senescence are critical for reproductive success and fitness. Although great progress has been made in identifying key genes regulating leaf senescence and elucidating the underlining mechanisms in the model plant Arabidopsis, there is still a gap to understanding the complex regulatory network. In this study, we discovered that Arabidopsis ANAC087 transcription factor (TF) positively modulated leaf senescence. Expression of ANAC087 was induced in senescing leaves and the encoded protein acted as a transcriptional activator. Both constitutive and inducible overexpression lines of ANAC087 showed earlier senescence than control plants, whereas T‐DNA insertion mutation and dominant repression of the ANAC087 delayed senescence rate. A quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) profiling showed that the expression of an array of senescence‐associated genes was upregulated in inducible ANAC087 overexpression plants including BFN1, NYE1, CEP1, RbohD, SAG13, SAG15, and VPEs, which are involved in programmed cell death (PCD), chlorophyll degradation and reactive oxygen species (ROS) accumulation. In addition, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation‐quantitative polymerase chain reaction (ChIP‐qPCR) assays demonstrated that ANAC087 directly bound to the canonical NAC recognition sequence (NACRS) motif in promoters of its target genes. Moreover, mutation of two representative target genes, BFN1 or NYE1 alleviated the senescence rate of ANAC087‐overexpression plants, suggesting their genetic regulatory relationship. Taken together, this study indicates that ANAC087 serves as an important regulator linking PCD, ROS, and chlorophyll degradation to leaf senescence.
Fundamental Neurochemistry Review: Incorporating a greater diversity of cell types, including microglia, in brain organoid cultures improves clinical translation
Authors: Wenzel Tyler J. , Le Jennifer , He Jim , Alcorn Jane , Mousseau Darrell D. 
Published: December 2022

AbstractBrain organoids have the potential to improve clinical translation, with the added benefit of reducing any extraneous use of experimental animals. As brain organoids are three‐dimensional in vitro constructs that emulate the human brain, they bridge in vitro and in vivo studies more appropriately than monocultures. Although many factors contribute to the failure of extrapolating monoculture‐based information to animal‐based experiments and clinical trials, for the purpose of this review, we will focus on glia (non‐neuronal brain cells), whose functions and transcriptome are particularly abnormal in monocultures. As discussed herein, glia require signals from—and contact with—other cell types to exist in their homeostatic state, which likely contributes to some of the differences between data derived from monocultures and data derived from brain organoids and even two‐dimensional co‐cultures. Furthermore, we highlight transcriptomic differences between humans and mice in regard to aging and Alzheimer's disease, emphasizing need for a model using the human genome—again, a benefit of brain organoids—to complement data derived from animals. We also identify an urgency for guidelines to improve the reporting and transparency of research using organoids. The lack of reporting standards creates challenges for the comparison and discussion of data from different articles. Importantly, brain organoids mark the first human model enabling the study of brain cytoarchitecture and development.image
Oxygen treatment reduces neurological deficits and demyelination in two animal models of multiple sclerosis
Authors: Amatruda Mario , Harris Kate , Matis Alina , Davies Andrew L. , McElroy Daniel , Clark Michael , Linington Christopher , Desai Roshni , Smith Kenneth J. 
Published: December 2022

AbstractAimsThe objective of the study is to explore the importance of tissue hypoxia in causing neurological deficits and demyelination in the inflamed CNS, and the value of inspiratory oxygen treatment, using both active and passive experimental autoimmune encephalomyelitis (EAE).MethodsNormobaric oxygen treatment was administered to Dark Agouti rats with either active or passive EAE, compared with room air‐treated, and naïve, controls.ResultsSevere neurological deficits in active EAE were significantly improved after just 1 h of breathing approximately 95% oxygen. The improvement was greater and more persistent when oxygen was applied either prophylactically (from immunisation for 23 days), or therapeutically from the onset of neurological deficits for 24, 48, or 72 h. Therapeutic oxygen for 72 h significantly reduced demyelination and the integrated stress response in oligodendrocytes at the peak of disease, and protected from oligodendrocyte loss, without evidence of increased oxidative damage. T‐cell infiltration and cytokine expression in the spinal cord remained similar to that in untreated animals.The severe neurological deficit of animals with passive EAE occurred in conjunction with spinal hypoxia and was significantly reduced by oxygen treatment initiated before their onset.ConclusionsSevere neurological deficits in both active and passive EAE can be caused by hypoxia and reduced by oxygen treatment. Oxygen treatment also reduces demyelination in active EAE, despite the autoimmune origin of the disease.
Substrate stiffness promotes dentinogenesis via LAMB1–FAK–MEK1/2 signaling axis
Authors: Bai Mingru , Chen Huiyu , Zhang Zhaowei , Liu Xiaoyu , Zhang Demao , Wang Chengling 
Published: December 2022

AbstractObjectivesIn vivo, the principal function of mechanosensitive odontoblasts is to synthesize and secrete the matrix which then calcifies and forms reactive dentin after exposure to appropriate stimuli. This study aims to develop the influence of mechanical factors on dentinogenesis based on odontoblasts, which contribute to reparative dentin formation.MethodsWe fabricated polydimethylsiloxane with different stiffnesses and seeded 17IIA11 odontoblast‐like cells on the substrates in different stiffnesses. Cell morphology was detected by scanning electron microscope, and the mineralization phenotype was detected by alkaline phosphatase staining and alizarin red staining, while expression levels of dentinogenesis‐related genes (including Runx2, Osx, and Alp) were assayed by qPCR. To explore mechanism, protein distribution and expression levels were detected by immunofluorescent staining, Western blotting, and immunoprecipitation.ResultsIn our results, during dentinogenesis, 17IIA11 odontoblast‐like cells appeared better extension on stiffer substrates. The binding between LAMB1 and FAK contributed to converting mechanical stimuli into biochemical signaling, thereby controlling mitogen‐activated protein kinase kinase 1/2 activity in stiffness‐driven dentinogenesis.ConclusionThe present study suggests odontoblast behaviors can be directly regulated by mechanical factors at cell–material interfaces, which offers fundamental mechanism in remodeling cell microenvironment, thereby contributing to physiological phenomena explanation and tissue engineering progress.
Deep learning‐assisted diagnosis of parotid gland tumors by using contrast‐enhanced CT imaging
Authors: Shen Xue‐Meng , Mao Liang , Yang Zhi‐Yi , Chai Zi‐Kang , Sun Ting‐Guan , Xu Yongchao , Sun Zhi‐Jun 
Published: December 2022

AbstractObjectivesImaging interpretation of the benignancy or malignancy of parotid gland tumors (PGTs) is a critical consideration prior to surgery in view of therapeutic and prognostic values of such discrimination. This study investigates the application of a deep learning‐based method for preoperative stratification of PGTs.Materials and MethodsUsing the 3D DenseNet‐121 architecture and a dataset consisting of 117 volumetric arterial‐phase contrast‐enhanced CT scans, we developed a binary classifier for PGT distinction and tested it. We compared the discriminative performance of the model on the test set to that of 12 junior and 12 senior head and neck clinicians. Besides, potential clinical utility of the model was evaluated by measuring changes in unassisted and model‐assisted performance of junior clinicians.ResultsThe model finally reached the sensitivity, specificity, PPV, NPV, F1‐score of 0.955 (95% CI 0.751–0.998), 0.667 (95% CI 0.241–0.940), 0.913 (95% CI 0.705–0.985), 0.800 (95% CI 0.299–0.989) and 0.933, respectively, comparable to that of practicing clinicians. Furthermore, there were statistically significant increases in junior clinicians' specificity, PPV, NPV and F1‐score in differentiating benign from malignant PGTs when unassisted and model‐assisted performance of junior clinicians were compared.ConclusionOur results provide evidence that deep learning‐based method may offer assistance for PGT's binary distinction.