bluuebunny/crossref_metadata_2025_split huggingface dataset:
A single center experience in 90 cases with nonimmune hydrops fetalis: diagnostic categories ‒ mostly aneuploidy and still often idiopathic
Authors: Sturm Julia , Milera Heiko , Essmann Stephanie , Fruth Anja , Jahn-Eimermacher Antje , Selig Mareike , Winter Jennifer , Seidmann Larissa , Kampmann Christoph , Kidszun André , Mildenberger Eva , Whybra Catharina 
Published: April 2022

Abstract

Objectives
The prognosis of nonimmune hydrops fetalis (NIHF) is still poor with a high mortality and morbidity rate despite progress in perinatal care. This study was designed to investigate etiology and outcome of NIHF.

Methods
A retrospective review of 90 NIHF cases from 2007 to 2019 was conducted at University Medical Center of the Johannes Gutenberg University, Mainz, Germany. Demographics, genetic results, prenatal and postnatal outcomes including one year survival as well as autopsy data were extracted. Etiology of hydrops was classified using 13 previously established categories. In 4 patients observed between 2016 and 2019, we used a next-generation-sequencing (NGS) panel for genetic evaluation.

Results
Ninety NIHF cases were identified, with a median gestational age (GA) at diagnosis of 14 weeks. There were 25 live-born infants with a median GA of 34 weeks at birth, 15 patients survived to one year. There was aneuploidy in more than one third of the cases. All 90 cases were subclassified into etiologic categories with chromosomal 35, idiopathic 15, syndromic 11, cardiovascular 9, inborn errors of metabolism 6, lymphatic dysplasia 3, thoracic 3, infections 3, gastrointestinal 3 and hematologic 2. The NGS panel was used in 4 cases and 4 diagnoses were made.

Conclusions
In 90 cases with NIHF we identified an aneuploidy in more than one third of the cases. Improved techniques, such as possibly specific genetic analysis, could reduce the high rate of unexplained cases of NIHF.
Language and political consciousness: explorations from the Philippines at the fin de siècle
Authors: Osborne Dana 
Published: April 2022

Abstract
At the turn of the twentieth century, the Philippine archipelago transitioned from nearly 400 years of colonial occupation under the Spanish to imperial occupation under the Americans. This analysis interrogates the dynamics through which the heterogeneous languages of the Philippine archipelago were maintained alongside state-sanctioned languages that over time came to create and sustain various forms of consciousness potentiated around the nexus of language. Using a theoretical foundation that intertwines Gramsci and Bakhtin’s understanding of the heteroglossic nature of language, the ways in which the interanimation of languages emerges as a potential site for the realization of certain forms of political consciousness is explored. This analysis interrogates the tensions emergent in forms of discourse linked to the question of language that gave rise to the contemporary linguistic situation in the Philippines today, both “from above” as well as “from below” at the fin de siècle.
Elucidating the role of silicon dioxide and titanium dioxide nanoparticles in mitigating the disease of the eggplant caused by Phomopsis vexans, Ralstonia solanacearum, and root-knot nematode Meloidogyne incognita
Authors: Khan Masudulla , Siddiqui Zaki A. , Parveen Aiman , Khan Azmat Ali , Moon Il Soo , Alam Mahboob 
Published: April 2022

Abstract
Nanoparticles (NPs) have a critical function in mitigating the disease of fruits and vegetables. In the present investigation, the effects of three levels of concentrations (0.05, 0.10, and 0.20 mg/mL) of titanium dioxide NPs (TiO2-NPs) and silicon dioxide NPs (SiO2-NPs) were investigated against fungus Phomopsis vexans, bacterium Ralstonia solanacearum, and Meloidogyne incognita (root-knot nematode). The present investigation’s findings found that the application of SiO2-NPs was more efficient against test pathogens in comparison to TiO2-NPs. The best result produced by SiO2-NPs against pathogenic strain was used in the molecular docking investigation with the protein of R. solanacearum to better understand the interaction of active amino acids with SiO2-NPs. The obtained results revealed that the administration of 0.20 mg/mL foliar spray of SiO2-NPs in plants with M. incognita improves up to 37.92% of shoot dry weight and increases 70.42% of chlorophyll content. P. vexans growth was suppressed by 41.2% with 0.62 mm of inhibition zone when SiO2-NPs were given at a dosage of 0.20 mg/mL. The reductions in egg hatching and M. incognita (J2) mortality were greater in SiO2-NPs than in TiO2-NPs. The results of scanning electron microscopy confirmed that the application of both NPs harmed test pathogens. The confocal study also showed the penetration of NPs among test pathogens.
Multi-objective optimization of injection molded parts with insert based on IFOA-GRNN-NSGA-II
Authors: Li Chunxiao , Fan Xiying , Guo Yonghuan , Liu Xin , Wang Changjing , Wang Dezhao 
Published: April 2022

Abstract
The physical properties of plastic products, such as local strength, wear resistance and electrical properties, can be improved by adding embedded parts in the appropriate position of the products, and the precision of plastic parts can also be improved. However, due to the addition of inserts, the flow and shrinkage around inserts will be affected. Compared with traditional injection molding products, the quality is difficult to predict. To solve this problem, the injection molded parts with inserts (electrostatic test box) was used as an example, according to the product structure, three objectives of volume shrinkage, warpage in the X direction, and warpage in the Z direction were optimized. A generalized regression neural network (GRNN) model was established with molding parameters as input and quality objectives as output. Improved fruit fly optimization algorithm (IFOA) was proposed to select the optimal smoothing parameters dynamically. Through the prediction of samples, the experimental results show that the model is superior to two comparative models. Non-dominated sorting genetic algorithm (NSGA-II) was used to solve the model, and the Pareto-optimal front was obtained. The entropy TOPSIS method was used to evaluate the Pareto-optimal front, and the optimal solution was obtained. The results show that IFOA-GRNN-NSGA is a reliable multi-objective optimization method.
Wertschöpfungskettenübergreifendes Fehlermanagement
Authors: Günther Robin , Beckschulte Sebastian , Wende Martin , Schmitt Robert H. 
Published: April 2022

Abstract
Jüngste technologische Entwicklungen lassen die Menge verfügbarer Daten entlang der Wertschöpfung und Produktnutzung ansteigen. Jedoch fehlt es derzeit an systematischen Ansätzen, um Daten über die eigenen Unternehmensgrenzen hinweg zu integrieren und im Sinne der Fehlerbearbeitung und -prävention zu verwerten. Im Rahmen des Forschungsprojekts „value chAIn“ wird die Erarbeitung eines wertschöpfungsübergreifenden Fehlermanagements angestrebt, welches sowohl eine Optimierung der Prozesse als auch die Verfügbarkeit und Leistung der Produkte einschließt.
Simulationsgestütztes Vorgehensmodell zur Realisierung einer Matrixfertigung
Authors: Kirchberger Manfred , Heeger Madeleine , Altay Alper , Liebrecht Christoph , Overbeck Leonard , Kandler Magnus , Lanza Gisela , Voigt Christian , Franke Jörg 
Published: April 2022

Abstract
Die Siemens AG in Karlsruhe stellt sich den aktuellen Herausforderungen einer steigenden Produktkomplexität bei höherer Variantenvielfalt mit reduzierter Losgröße und kürzeren Produktlebenszyklen. Für die Umstrukturierung ihres Fertigungssystems zu einer „Matrixproduktion im Fluss“ wurde ein Vorgehensmodell zur Neuplanung und Umstrukturierung entworfen, welches als Leitfaden hierfür dienen soll. Unterstützt durch den Digitalen Zwilling und Simulationsstudien zeigt sich eine optimierte Modulkonfiguration für eine hochflexible Fertigung mit fahrerlosen Transportfahrzeugen. Herausforderungen wie die komplexe Fertigungssteuerung können im Lebenszyklus durch den ganzheitlichen Digitalen Zwilling unterstützt werden.
Prediction Error Determines Whether NMDA Receptors in the Basolateral Amygdala Complex Are Involved in Pavlovian Fear Conditioning
Authors: Williams-Spooner Matthew J. , Delaney Andrew J. , Westbrook R. Frederick , Holmes Nathan M. 
Published: April 2022

It is widely accepted that activation of NMDA receptors (NMDAR) is necessary for the formation of fear memories in the basolateral amygdala complex (BLA). This acceptance is based on findings that blockade of NMDAR in the BLA disrupts Pavlovian fear conditioning in rodents when initially innocuous stimuli are paired with aversive and unexpected events (surprising foot shock). The present study challenges this acceptance by showing that the involvement of NMDAR in Pavlovian fear conditioning is determined by prediction errors in relation to aversive events. In the initial experiments, male rats received a BLA infusion of the NMDAR antagonist, D-AP5 and were then exposed to pairings of a novel target stimulus and foot shock. This infusion disrupted acquisition of fear to the target when the shock was surprising (experiments 1a, 1b, 2a, 2b, 3a, and 3b) but spared fear to the target when the shock was expected based on the context, time and other stimuli that were present (experiments 1a and 1b). Under the latter circumstances, fear to the target required activation of calcium-permeable AMPAR (CP-AMPA; experiments 4a, 4b, and 4c), which, using electrophysiology, were shown to regulate the activity of interneurons in the BLA (experiment 5). Thus, NMDAR activation is not required for fear conditioning when danger occurs as expected given the context, time and stimuli present, but is required for fear conditioning when danger occurs unexpectedly. These findings are related to current theories of NMDAR function and ways that prediction errors might influence the substrates of fear memory formation in the BLA.SIGNIFICANCE STATEMENTIt is widely accepted that NMDA receptors (NMDAR) in the basolateral amygdala complex (BLA) are activated by pairings of a conditioned stimulus (CS) and an aversive unconditioned (US) stimulus, leading to the synaptic changes that underlie formation of a CS-US association. The present findings are significant in showing that this theory is incomplete. When the aversive US is unexpected, animals encode all features of the situation (context, time and stimuli present) as a new fear/threat memory, which is regulated by NMDAR in the BLA. However, when the US is expected based on the context, time and stimuli present, the new fear memory is assimilated into networks that represent those features, which occurs independently of NMDAR activation in the BLA.
Plasmodium sporozoite disintegration during skin passage limits malaria parasite transmission
Authors: Kehrer Jessica , Formaglio Pauline , Muthinja Julianne Mendi , Weber Sebastian , Baltissen Danny , Lance Christopher , Ripp Johanna , Grech Janessa , Meissner Markus , Funaya Charlotta , Amino Rogerio , Frischknecht Friedrich 
Published: April 2022

AbstractDuring transmission of malaria‐causing parasites from mosquitoes to mammals, Plasmodium sporozoites migrate rapidly in the skin to search for a blood vessel. The high migratory speed and narrow passages taken by the parasites suggest considerable strain on the sporozoites to maintain their shape. Here, we show that the membrane‐associated protein, concavin, is important for the maintenance of the Plasmodium sporozoite shape inside salivary glands of mosquitoes and during migration in the skin. Concavin‐GFP localizes at the cytoplasmic periphery and concavin(−) sporozoites progressively round up upon entry of salivary glands. Rounded concavin(−) sporozoites fail to pass through the narrow salivary ducts and are rarely ejected by mosquitoes, while normally shaped concavin(−) sporozoites are transmitted. Strikingly, motile concavin(−) sporozoites disintegrate while migrating through the skin leading to parasite arrest or death and decreased transmission efficiency. Collectively, we suggest that concavin contributes to cell shape maintenance by riveting the plasma membrane to the subtending inner membrane complex. Interfering with cell shape maintenance pathways might hence provide a new strategy to prevent a malaria infection.
Features of the exogenic development of Passalurus ambiguus (Nematoda, Oxyuroidae) at different temperature regimes
Authors: Yevstafieva V. , Khorolskyi A. , Kravchenko S. , Melnychuk V. , Nikiforova O. , Reshetylo O. 
Published: April 2022

Passalurosis is a prevalent disease among helminthiases of domestic rabbits. This invasion is caused by the nematode Passalurus ambiguus (Nematoda, Oxyuroidae), which is cosmopolitan and localized in the cecum and colon of rabbits. Passalurosis is highly contagious and capable of unlimited spread, due to the biological characteristics of its pathogen, such as the conditions that ensure maximum preservation of parasites at exogenous stages of their development. Experimental research in the laboratory established the timing of development of P. ambiguus eggs isolated from the gonads of female helminths, depending on temperature regimes and features of their growth and development. According to the morphological features of Passalurus eggs, four stages were distinguished in their exogenous development: zygote, cleavage and formation of blastomeres, formation of larvae and motile larvae. Depending on the cultivation temperature, the duration of embryogenesis ranged from 4 to 9 days, and egg viability ranged from 59.3% to 72.7%. The most favourable temperature regime for the development of P. ambiguus eggs was the temperature of 35 °C, at which 72.7% of eggs with motile larvae were formed within 4 days. At this temperature, the zygote stage lasted for 1 day of cultivation, the stage of cleaving and formation of blastomeres occurred on days 1–2, the stage of larval formation on days 1–3, and the stage of formation of motile larva on days 3–4. At lower temperatures, the term of development of Passalurus eggs increased, and the number of viable eggs decreased. At temperatures of 30 °С and 25 °С, the development of Passalurus eggs took place during 5 and 7 days, respectively, and the viability was 66.7% and 62.7%. At these temperatures, the zygote stage lasted 1–2 and 1–3 days, the stage of cleaving and formation of blastomeres occurred on days 1–3 and 1–4, the stage of larval formation lasted from days 2–4 and 2–6, and the stage of formation of motile larvae took place on days 3–5 and 4–7, respectively. The least favourable temperature for the development of P. ambiguus eggs was the temperature of 20 °С, at which the formation of motile larvae occurred in 9 days, and their viability was only 59.3%. At this temperature, the zygote stage lasted 1–4 days, the stage of cleaving and blastomere formation occurred on days 2–6, and the larval formation stage on days 3–8, and the motile larval stage happened on days 5–9. The growth and development of P. ambiguus eggs was accompanied by significant changes in morphometric parameters, such as the increase in egg width and thinning of egg shell at the egg shell plug. The obtained data will allow preventive measures to be effectively implemented on rabbit farms that are susceptible to pinworms, taking into account the terms of exogenous development of pathogens in different seasons.
The coupled strength and toughness of interconnected and interpenetrating multi-material gyroids
Authors: Indurkar Padmeya P. , Shaikeea Angkur , Xu Zhenpeng , Cui Huachen , Zheng Xiaoyu , Deshpande Vikram 
Published: April 2022

Abstract
The growth of additive manufacturing technologies has spurred interest in examining multi-material micro-architected materials for filling the so-called white spaces in the Ashby strength versus toughness plots. We investigate this problem using interconnected and interpenetrating double gyroids comprising ductile and brittle phases as an exemplar. Both strength and toughness at the initiation of crack growth are shown to vary non-monotonically with the volume fraction of the two phases and multi-material double gyroids significantly outperform their single material counterparts. However, we establish that at a given relative density, the strength and toughness cannot be simultaneously enhanced for architecture designs, which include varying gyroid orientations, phase volume fractions, and the unit cell length scales of the two phases. Intriguingly, even crack flank bridging by the ductile phase during crack growth is insufficient to overcome this inherent property of the interpenetrating gyroids. Our conclusion is that multi-material interpenetrating micro-architected solids are unlikely to outperform single material non-interpenetrating lattices from a strength–toughness perspective but rather become optimal when multi-functionality is required.

Impact statement
The integration of materials and architectural features at multiple scales into structural mechanics gave us structural designs such as the Eiffel Tower. The explosion of additive manufacturing methods has opened new avenues for the invention of multi-material micro-architected materials that simultaneously possess high strength and toughness at a low density, and thereby can fill the so-called “white spaces” in the Ashby strength–toughness space. The idea is to construct three-dimensional materials with a network of crack arrestors like in rip-stop nylon and break the link between toughness and strength. We use interconnected and interpenetrating double gyroids comprising ductile and brittle phases as an exemplar to investigate the opportunities of such designs. Intriguingly, from a perspective based solely on strength and toughness, we show that multi-material micro-architectures cannot outperform their single material counterparts at a given relative density. In fact, in most designs the coupling between the two phases is non-synergistic. Rather, we argue that multi-material designs such as those used in rip-stop nylon are driven by multi-functional considerations beyond mechanical properties.

Graphical abstract
School water, sanitation, and hygiene inequalities: a bane of sustainable development goal six in Nigeria
Authors: Wada Ojima Zechariah , Olawade David Bamidele , Oladeji Eunice Oluwafolakemi , Amusa Aminat Opeyemi , Oloruntoba Elizabeth Omoladun 
Published: April 2022

Abstract
Objectives
The importance of school water, sanitation, and hygiene (WASH) in achieving the Sustainable Development Goal targets 6.1 and 6.2 in developing countries cannot be overemphasized. However, widespread WASH inequalities remain an impediment to achieving the targets by 2030. Hence, this study was conducted to examine current school-WASH disparities among public and private schools in a low-income Nigerian community using mixed methods.

Methods
The cross-sectional survey utilized multi-stage sampling to select 400 students from five public and five private schools in Akinyele, Ibadan. Semi-structured questionnaires and observational checklists were used to obtain data. Inferential statistics were measured at a 95% confidence interval. Independent variables like the students’ sociodemographic characteristics, school type, and available WASH facilities were associated with dependent variables like respondents’ hand hygiene and sanitation practices and WASH-associated knowledge and attitude to examine existing inequalities.

Results
Classifying the available WASH facilities based on the WHO/UNICEF Joint Monitoring Programme, none of the public schools provided any sanitation and hygiene service, while all the private schools provided both services. Furthermore, the private-school students had significantly better WASH knowledge (p<0.001; Ƞ2p=0.152) and attitude (p<0.001; Ƞ2p=0.036) compared with the public-school students. Also, a significantly higher portion of public-school students practiced open defecation at school (p<0.001; odds ratio (OR)=7.4; confidence interval (CI)=4.1–13.5) and at home (p<0.001; OR=7.8; CI=3.7–16.7).

Conclusion
WASH disparities among socioeconomic groups remain a persistent challenge. Sole reliance on the Government to narrow the inequalities has persistently proven unfruitful. There is a need to empower local community stakeholders to facilitate sustainable school-WASH interventions.
Cataract Surgery Results after Penetrating Keratoplasty
Authors: Tanash M. A. , Frolov M. A. , Kumar V. , Ababneh L. T. , Alqudah A. A. 
Published: April 2022

Objective. To compare clinical and refractive outcomes of cataract surgery by phacoemulsification and by extracapsular cataract extraction with implantation of foldable and rigid intraocular lenses in patients previously having undergone penetrating keratoplasty (PK).Patients and methods. This is a retrospective study. Medical records of all patients admitted for cataract surgery between 2010 and 2018 to the King Abdullah University Hospital (KAUH) were reviewed and medical records of patients who underwent cataract surgery after PK were analyzed. Medical records of 95 patients (58 males and 37 females; average age 40.0 ± 19.9 years) were selected. Depending upon the type of surgical technique applied, the cases were divided into two groups: first group included 60 patients who underwent cataract surgery by phacoemulsification with implantation of different foldable intraocular lenses (IOL), the second group included 35 patients who underwent cataract surgery by extracapsular cataract extraction (ECCE) with implantation of a polymethylmethacrylate (PMMA) IOL. The choice of ECCE technique with implantation of rigid IOLs was made if the endothelial cell density after PK was less than 2200 cells/mm2 and cataracts were of 4 and 5 grades to exclude additional trauma to the endothelium by ultrasound energy. Demographic and clinical data were collected. The R statistical program and SPSS were used for data analysis. The P value < 0.05 was considered statistically significant.Results. Analysis showed that PK was performed mainly due to 4 indications: keratoconus (KC) — 77 % cases, herpetic keratopathy — 7 % cases, Eye trauma — 5 % cases, previous graft rejection — 5 % cases and Stevens–Johnson syndrome — 1 % cases. The mean best corrected visual acuity (BCVA) in the first group improved from 0.40 ± 0.05 to 0.80 ± 0.05 postoperatively. The mean BCVA in the second group improved from 0.30 ± 0.05 to 0.70 ± 0.05. In the late postoperative period BCVA with foldable IOLs was higher by 0.10 ± 0.05, as compared with the results obtained with the rigid IOLs. Mean intraocular pressure (IOP) changed from 15.1 ± 4.9 before PK to 16.3 ± 5.3 after PK.Conclusion. KC was the most common indication for PK in Jordan. Results of our analysis showed that cataract surgery could be successfully performed in eyes after PK with favorable results and improvement in patients’ quality of life. Cataract surgery by phacoemulsification with implantation of foldable IOLs results in higher (BCVA) compared to ECCE with implantation of PMMA IOLs. Unless contraindicated otherwise, phacoemulsification technique with foldable IOL implantation should be given preference over ECCE with implantation of rigid PMMA IOLs in visual rehabilitation of cataract patients after PK.