bluuebunny/crossref_metadata_2025_split huggingface dataset:
Standardized Health data and Research Exchange (SHaRE): promoting a learning health system
Authors: Davis Sierra , Ehwerhemuepha Louis , Feaster William , Hackman Jeffrey , Morizono Hiroki , Kanakasabai Saravanan , Mosa Abu Saleh Mohammad , Parker Jerry , Iwamoto Gary , Patel Nisha , Gasparino Gary , Kane Natalie , Hoffman Mark A 
Published: January 2022

Abstract
Aggregate de-identified data from electronic health records (EHRs) provide a valuable resource for research. The Standardized Health data and Research Exchange (SHaRE) is a diverse group of US healthcare organizations contributing to the Cerner Health Facts (HF) and Cerner Real-World Data (CRWD) initiatives. The 51 facilities at the 7 founding organizations have provided data about more than 4.8 million patients with 63 million encounters to HF and 7.4 million patients and 119 million encounters to CRWD. SHaRE organizations unmask their organization IDs and provide 3-digit zip code (zip3) data to support epidemiology and disparity research. SHaRE enables communication between members, facilitating data validation and collaboration as we demonstrate by comparing imputed EHR module usage to actual usage. Unlike other data sharing initiatives, no additional technology installation is required. SHaRE establishes a foundation for members to engage in discussions that bridge data science research and patient care, promoting the learning health system.
Attention-based spatial–temporal neural network for accurate phase recognition in minimally invasive surgery: feasibility and efficiency verification
Authors: Shi Pan , Zhao Zijian , Liu Kaidi , Li Feng 
Published: January 2022

Abstract
Laparoscopic surgery, as a representative minimally invasive surgery (MIS), is an active research area of clinical practice. Automatic surgical phase recognition of laparoscopic videos is a vital task with the potential to improve surgeons’ efficiency and has gradually become an integral part of computer-assisted intervention systems in MIS. However, the performance of most methods currently employed for surgical phase recognition is deteriorated by optimization difficulties and inefficient computation, which hinders their large-scale practical implementation. This study proposes an efficient and novel surgical phase recognition method using an attention-based spatial–temporal neural network consisting of a spatial model and a temporal model for accurate recognition by end-to-end training. The former subtly incorporates the attention mechanism to enhance the model’s ability to focus on the key regions in video frames and efficiently capture more informative visual features. In the temporal model, we employ independently recurrent long short-term memory (IndyLSTM) and non-local block to extract long-term temporal information of video frames. We evaluated the performance of our method on the publicly available Cholec80 dataset. Our attention-based spatial–temporal neural network purely produces the phase predictions without any post-processing strategies, achieving excellent recognition performance and outperforming other state-of-the-art phase recognition methods.
Polycomb proteins control floral determinacy by H3K27me3-mediated repression of pluripotency genes in Arabidopsis thaliana
Authors: Müller-Xing Ralf , Ardiansyah Rhomi , Xing Qian , Faivre Léa , Tian Jingjing , Wang Guohua , Zheng Yucai , Wang Xue , Jing Tingting , de Leau Erica , Chen Song , Chen Su , Schubert Daniel , Goodrich Justin 
Published: January 2022

Abstract
Polycomb group (PcG) protein-mediated histone methylation (H3K27me3) controls the correct spatiotemporal expression of numerous developmental regulators in Arabidopsis. Epigenetic silencing of the stem cell factor gene WUSCHEL (WUS) in floral meristems (FMs) depends on H3K27me3 deposition by PcG proteins. However, the role of H3K27me3 in silencing of other meristematic regulator and pluripotency genes during FM determinacy has not yet been studied. To this end, we report the genome-wide dynamics of H3K27me3 levels during FM arrest and the consequences of strongly depleted PcG activity on early flower morphogenesis including enlarged and indeterminate FMs. Strong depletion of H3K27me3 levels results in misexpression of the FM identity gene AGL24, which partially causes floral reversion leading to ap1-like flowers and indeterminate FMs ectopically expressing WUS and SHOOT MERISTEMLESS (STM). Loss of STM can rescue supernumerary floral organs and FM indeterminacy in H3K27me3-deficient flowers, indicating that the hyperactivity of the FMs is at least partially a result of ectopic STM expression. Nonetheless, WUS remained essential for the FM activity. Our results demonstrate that PcG proteins promote FM determinacy at multiple levels of the floral gene regulatory network, silencing initially floral regulators such as AGL24 that promotes FM indeterminacy and, subsequently, meristematic pluripotency genes such as WUS and STM during FM arrest.
Ivermectin for COVID-19: Addressing Potential Bias and Medical Fraud
Authors: Hill Andrew , Mirchandani Manya , Pilkington Victoria 
Published: January 2022

Abstract
Ivermectin has become a controversial potential medicine for coronavirus disease 2019. Some early studies suggested clinical benefits in treatment of infection. However, the body of evidence includes studies of varying quality. Furthermore, some trials have now been identified as potentially fraudulent. We present a subgroup meta-analysis to assess the effects of stratifying by trial quality on the overall results. The stratification is based on the Cochrane Risk of Bias measures and raw data analysis where possible. The results suggest that the significant effect of ivermectin on survival was dependent on largely poor-quality studies. According to the potentially fraudulent study (risk ratio [RR], 0.08; 95% CI, 0.02–0.35), ivermectin improved survival ~12 times more in comparison with low-risk studies (RR, 0.96; 95% CI, 0.56–1.66). This highlights the need for rigorous quality assessments, for authors to share patient-level data, and for efforts to avoid publication bias for registered studies. These steps are vital to facilitate accurate conclusions on clinical treatments.
Public Health in a Federation: Lessons from the Spanish Influenza in Australia
Authors: Holbrook Carolyn 
Published: January 2022

Summary
Public health policy has been identified by scholars as a principal means by which the state has expanded its control over human populations. Yet, as the COVID-19 pandemic has shown, public health responses do not necessarily reinforce the authority and prestige of the state, even while governments employ strict measures such as lockdowns and border closures. This article examines arguments about the nation-making effects of public health measures through an examination of the Spanish influenza outbreak in the recently federated Australian nation during 1919. It examines the effort of the central government to co-ordinate quarantine and other public health measures in the face of serious tensions within the Australian federation. In doing so, the article suggests a need to think more subtly about the role of ‘bio-political’ events such as public health crises in consolidating state control and fostering exclusionary forms of nationalism. These lessons apply particularly to federal nation-states.
Justice beliefs and cultural values predict support for COVID-19 vaccination and quarantine behavioral mandates: a multilevel cross-national study
Authors: Lucas Todd , Manning Mark , Strelan Peter , Kopetz Catalina , Agostini Maximilian , Bélanger Jocelyn J , Gützkow Ben , Kreienkamp Jannis , Leander N Pontus ,   PsyCorona Collaboration
Published: January 2022

Abstract
Understanding how individual beliefs and societal values influence support for measures to prevent SARS-CoV-2 transmission is vital to developing and implementing effective prevention policies. Using both Just World Theory and Cultural Dimensions Theory, the present study considered how individual-level justice beliefs and country-level social values predict support for vaccination and quarantine policy mandates to reduce SARS-CoV-2 transmission. Data from an international survey of adults from 46 countries (N = 6424) were used to evaluate how individual-level beliefs about justice for self and others, as well as national values—that is, power distance, individualism, masculinity, uncertainty avoidance, long-term orientation, and indulgence—influence support for vaccination and quarantine behavioral mandates. Multilevel modeling revealed that support for vaccination and quarantine mandates were positively associated with individual-level beliefs about justice for self, and negatively associated with country-level uncertainty avoidance. Significant cross-level interactions revealed that beliefs about justice for self were associated more strongly with support for mandatory vaccination in countries high in individualism, whereas beliefs about justice for others were more strongly associated with support for vaccination and quarantine mandates in countries high in long-term orientation. Beliefs about justice and cultural values can independently and also interactively influence support for evidence-based practices to reduce SARS-CoV-2 transmission, such as vaccination and quarantine. Understanding these multilevel influences may inform efforts to develop and implement effective prevention policies in varied national contexts.
Generation of intense dissipation in high Reynolds number turbulence
Authors: Buaria Dhawal , Pumir Alain , Bodenschatz Eberhard 
Published: January 2022

Intense fluctuations of energy dissipation rate in turbulent flows result from the self-amplification of strain rate via a quadratic nonlinearity, with contributions from vorticity (via the vortex stretching mechanism) and pressure-Hessian—which are analysed here using direct numerical simulations of isotropic turbulence on up to

12

288
3

grid points, and Taylor-scale Reynolds numbers in the range 140–1300. We extract the statistics involved in amplification of strain and condition them on the magnitude of strain. We find that strain is self-amplified by the quadratic nonlinearity, and depleted via vortex stretching, whereas pressure-Hessian acts to redistribute strain fluctuations towards the mean-field and hence depletes intense strain. Analysing the intense fluctuations of strain in terms of its eigenvalues reveals that the net amplification is solely produced by the third eigenvalue, resulting in strong compressive action. By contrast, the self-amplification acts to deplete the other two eigenvalues, whereas vortex stretching acts to amplify them, with both effects cancelling each other almost perfectly. The effect of the pressure-Hessian for each eigenvalue is qualitatively similar to that of vortex stretching, but significantly weaker in magnitude. Our results conform with the familiar notion that intense strain is organized in sheet-like structures, which are in the vicinity of, but never overlap with tube-like regions of intense vorticity due to fundamental differences in their amplifying mechanisms.

This article is part of the theme issue ‘Scaling the turbulence edifice (part 1)’.
Weak stability and closure in turbulence
Authors: De Lellis C. , Székelyhidi L. 
Published: January 2022

We survey recent results in the mathematical literature on the equations of incompressible fluid dynamics, highlighting common themes and how they might contribute to the understanding of some phenomena in the theory of fully developed turbulence.
This article is part of the theme issue ‘Scaling the turbulence edifice (part 1)’.
Age-Induced Changes in Mu Opioid Receptor Signaling in the Midbrain Periaqueductal Gray of Male and Female Rats
Authors: Fullerton Evan F. , Karom Mary C. , Streicher John M. , Young Larry J. , Murphy Anne Z. 
Published: January 2022

AbstractThe analgesic effects of opioids are attenuated in aged rats. Opioids such as morphine have decreased analgesic potency (but not efficacy) in aged rodents compared to adults; however, the neural mechanisms underlying this attenuated response are not yet known. The present study investigated the impact of advanced age and biological sex on opioid signaling in the ventrolateral periaqueductal gray (vlPAG) in the presence of chronic inflammatory pain. Assays measuring mu-opioid receptor (MOR) radioligand binding, GTPγS binding, receptor phosphorylation, cAMP inhibition, and regulator of G-protein signaling (RGS) protein expression were performed on vlPAG tissue from adult (2-3mos) and aged (16-18mos) male and female rats. Persistent inflammatory pain was induced by intraplantar injection of Complete Freund’s Adjuvant (CFA). Adult males exhibited the highest MOR binding potential and the highest G-protein activation (activation efficiency ratio) in comparison to aged males and females (adult and aged). No impact of advanced age or sex on MOR phosphorylation state was observed. DAMGO-induced cAMP inhibition was highest in the vlPAG of adult males compared to aged males and females (adult and aged). vlPAG levels of RGS4 and RGS9-2, critical for terminating G-protein signaling, were assessed using RNAscope. Adult rats (both males and females) exhibited lower levels of vlPAG RGS4 and RGS9-2 mRNA expression compared to aged males and females. The observed age-related reductions in vlPAG MOR binding potential, G-protein activation efficiency, and cAMP inhibition, along with the observed age-related increases in RGS4 and RGS9-2 vlPAG expression, provide potential mechanisms whereby the potency of opioids is decreased in the aged population. These results have significant implications for pain management in this population.HighlightsAged males and females (adult and aged) exhibit reduced vlPAG μ-opioid receptor binding potential compared to adult males.Aged males and females (adult and aged) exhibit reduced opioid-induced vlPAG G-protein activation compared to adult males.Aged males and females (adult and aged) exhibit reduced vlPAG MOR mediated cAMP inhibition compared to adult males.Aged rats (males and females) exhibit increased vlPAG mRNA expression of Regulator of G-Protein Signaling (RGS) proteins RGS4 and RGS9-2 compared to adult rats (males and females), which may explain the reduced receptor signaling observed in aged animals.These coordinate decreases in opioid receptor signaling may explain the previously reported reduced potency of opioids to produce pain relief in females and aged rats.
Endothelial Nitric Oxide Synthase (eNOS) S1176 phosphorylation status governs atherosclerotic lesion formation
Authors: Nguyen Tung D. , Rahman Nur-Taz , Sessa William C. , Lee Monica Y. 
Published: January 2022

ABSTRACTObjectiveWe have previously demonstrated thein vivoimportance of the Akt-eNOS substrate-kinase relationship, as defective postnatal angiogenesis characteristic of global Akt1-null mice is rescued when bred to ‘gain-of-function’ eNOS S1176D mutant mice. While multiple studies support the vascular protective role of endothelial NO generation, the causal role of Akt1-dependent eNOS S1176 phosphorylation during atherosclerotic plaque formation is not yet clear.Approach & ResultsWe herein bred congenic ‘loss-of-function’ eNOS S1176A and ‘gain-of-function’ eNOS S1176D mutant mice to the exacerbated atherogenic Akt1-/-; ApoE-/-double knockout mice to definitively test the importance of Akt-mediated eNOS S1176 phosphorylation during atherogenesis. We find that a single amino acid substitution at the eNOS S1176 phosphorylation site yields divergent effects on atherosclerotic plaque formation, as an eNOS phospho-mimic aspartate (D) substitution at S1176 leads to favorable lipid profiles and decreased indices of atherosclerosis, even when on a proatherogenic Akt1 global deletion background. Conversely, mice harboring an unphosphorylatable mutation to alanine (S1176A) result in increased plasma lipids, increased lesion formation and cellular apoptosis, phenocopying the physiological consequence of eNOS deletion and/or impaired enzyme function. Furthermore, gene expression analyses of whole aortas indicate a combinatorial detriment from NO deficiency and Western Diet challenge, as ‘loss-of-function’ eNOS SA mice on a Western diet present a unique expression pattern indicative of augmented T-cell activity when compared to eNOS S1176D mice.ConclusionsBy using genetic epistasis approaches, we conclusively demonstrate that Akt-mediated eNOS S1176 phosphorylation and subsequent eNOS activation remains to be the most physiologically relevant method of NO production to promote athero-protective effects.
Distinct Inhibitory Pathways Control Velocity and Directional Tuning in the Retina
Authors: Summers Mathew T. , Feller Marla B. 
Published: January 2022

SummaryThe sensory periphery is responsible for detecting ethologically relevant features of the external world, using compact, predominantly feedforward circuits. Visual motion is a particularly prevalent sensory feature, the presence of which can be a signal to enact diverse behaviors ranging from gaze stabilization reflexes, to predator avoidance or prey capture. To understand how the retina constructs the distinct neural representations required for these diverse behaviors, we investigated two circuits responsible for encoding different aspects of image motion: ON and ON-OFF direction selective ganglion cells (DSGCs). Using a combination of 2-photon targeted whole cell electrophysiology, pharmacology, and conditional knockout mice, we show that distinct inhibitory pathways independently control tuning for motion velocity and motion direction in these two cell types. We further employ dynamic clamp and numerical modeling techniques to show that asymmetric inhibition provides a velocity-invariant mechanism of directional tuning, despite the strong velocity dependence of classical models of direction selectivity. We therefore demonstrate that invariant representations of motion features by inhibitory interneurons act as computational building blocks to construct distinct, behaviorally relevant signals at the earliest stages of the visual system.
Comparison of neural population dynamics in the regression subspace between continuous and categorical task parameters
Authors: Chen He , Kunimatsu Jun , Oya Tomomichi , Imaizumi Yuri , Hori Yukiko , Matsumoto Masayuki , Minamimoto Takafumi , Naya Yuji , Yamada Hiroshi 
Published: January 2022

AbstractNeural population dynamics, presumably fundamental computational units in the brain, provide a key framework for understanding information processing in the sensory, cognitive, and motor functions. However, neural population dynamics is not explicitly related to the conventional analytic framework for single-neuron activity, i.e., representational models that analyze neuronal modulations associated with cognitive and motor parameters. In this study, we applied a recently developed state-space analysis to incorporate the representational models into the dynamic model in combination with these parameters. We compared neural population dynamics between continuous and categorical task parameters during two visual recognition tasks, using the datasets originally designed for a single-neuron approach. We successfully extracted neural population dynamics in the regression subspace, which represent modulation dynamics for both continuous and categorical task parameters with reasonable temporal characteristics. Furthermore, we combined the classical optimal-stimulus analysis paradigm for the single-neuron approach (i.e., stimulus identified as maximum neural responses) into the dynamic model, and found that the most prominent modulation dynamics at the lower dimension were derived from these optimal responses. Thus, our approach provides a unified framework for incorporating knowledge acquired with the single-neuron approach into the dynamic model as a standard procedure for describing neural modulation dynamics in the brain.
Evaluation of an emergency safe supply drugs and managed alcohol program in COVID-19 isolation hotel shelters for people experiencing homelessness
Authors: Brothers Thomas D. , Leaman Malcolm , Bonn Matthew , Lewer Dan , Atkinson Jacqueline , Fraser John , Gillis Amy , Gniewek Michael , Hawker Leisha , Hayman Heather , Jorna Peter , Martell David , O’Donnell Tiffany , Rivers-Bowerman Helen , Genge Leah 
Published: January 2022

ABSTRACTBackgroundDuring a COVID-19 outbreak in the congregate shelter system in Halifax, Nova Scotia, Canada, a multidisciplinary health care team provided an emergency “safe supply” of pharmaceutical-grade medications and beverage-grade alcohol to facilitate isolation in COVID-19 hotel shelters for residents who are dependent on these substances. We aimed to evaluate (a) substances and dosages provided, and (b) effectiveness and safety of the program.MethodsWe retrospectively reviewed medical records of all COVID-19 isolation hotel shelter residents during May 2021. We extracted data on medication and alcohol dosages provided each day. The primary outcome was residents prematurely leaving isolation against public health orders. Adverse events included (a) overdose; (b) intoxication; and (c) diversion, selling, or sharing of medications or alcohol.ResultsOver 25 days, 77 isolation hotel residents were assessed (mean age 42 ± 14 years; 24% women). Sixty-two (81%) residents were provided medications, alcohol, or cigarettes. Seventeen residents (22%) received opioid agonist treatment medications (methadone, buprenorphine, or slow-release oral morphine) and 27 (35%) received hydromorphone tablets. Thirty-one (40%) residents received stimulant tablets with methylphenidate (27; 35%), dextroamphetamine (8; 10%), or lisdexamfetamine (2; 3%). Six residents (8%) received benzodiazepines. Forty-two (55%) residents received alcohol, including 41 (53%) with strong beer, three (3%) with wine, and one (1%) with hard liquor. Over 14 days in isolation, mean daily dosages increased of hydromorphone (45 ± 32 to 57 ± 42mg), methylphenidate (51 ± 28 to 77 ± 37mg), dextroamphetamine (33 ± 16 to 46 ± 13mg), and alcohol (12.3 ± 7.6 to 13.0 ± 6.9 standard drinks). Six residents (8%) left isolation prematurely, but four of those residents returned. Over 1,059 person-days in isolation, there were zero overdoses. Documented concerns regarding intoxication occurred six times (0.005 events/person-day) and medication diversion or sharing three times (0.003 events/person-day).ConclusionsAn emergency safe supply and managed alcohol program, paired with housing, was associated with low rates of adverse events and high rates of successful completion of the 14-day isolation period in COVID-19 isolation hotel shelters. This supports the effectiveness and safety of emergency safe supply prescribing and managed alcohol in this setting.