bluuebunny/crossref_metadata_2025_split huggingface dataset:
Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis
Authors: Mestre-Farràs Neus , Guerrero Santiago , Bley Nadine , Rivero Ezequiel , Coll Olga , Borràs Eva , Sabidó Eduard , Indacochea Alberto , Casillas-Serra Carlos , Järvelin Aino I , Oliva Baldomero , Castello Alfredo , Hüttelmaier Stefan , Gebauer Fátima 
Published: July 2022

Abstract
RNA-binding proteins (RBPs) have been relatively overlooked in cancer research despite their contribution to virtually every cancer hallmark. Here, we use RNA interactome capture (RIC) to characterize the melanoma RBPome and uncover novel RBPs involved in melanoma progression. Comparison of RIC profiles of a non-tumoral versus a metastatic cell line revealed prevalent changes in RNA-binding capacities that were not associated with changes in RBP levels. Extensive functional validation of a selected group of 24 RBPs using five different in vitro assays unveiled unanticipated roles of RBPs in melanoma malignancy. As proof-of-principle we focused on PDIA6, an ER-lumen chaperone that displayed a novel RNA-binding activity. We show that PDIA6 is involved in metastatic progression, map its RNA-binding domain, and find that RNA binding is required for PDIA6 tumorigenic properties. These results exemplify how RIC technologies can be harnessed to uncover novel vulnerabilities of cancer cells.
Exploration of DNA processing features unravels novel properties of ICE conjugation in Gram-positive bacteria
Authors: Laroussi Haifa , Aoudache Yanis , Robert Emilie , Libante Virginie , Thiriet Louise , Mias-Lucquin Dominique , Douzi Badreddine , Roussel Yvonne , Chauvot de Beauchêne Isaure , Soler Nicolas , Leblond-Bourget Nathalie 
Published: July 2022

Abstract
Integrative and conjugative elements (ICEs) are important drivers of horizontal gene transfer in prokaryotes. They are responsible for antimicrobial resistance spread, a major current health concern. ICEs are initially processed by relaxases that recognize the binding site of oriT sequence and nick at a conserved nic site. The ICESt3/Tn916/ICEBs1 superfamily, which is widespread among Firmicutes, encodes uncanonical relaxases belonging to a recently identified family called MOBT. This family is related to the rolling circle replication initiators of the Rep_trans family. The nic site of these MOBT relaxases is conserved but their DNA binding site is still unknown. Here, we identified the bind site of RelSt3, the MOBT relaxase from ICESt3. Unexpectedly, we found this bind site distantly located from the nic site. We revealed that the binding of the RelSt3 N-terminal HTH domain is required for efficient nicking activity. We also deciphered the role of RelSt3 in the initial and final stages of DNA processing during conjugation. Especially, we demonstrated a strand transfer activity, and the formation of covalent DNA-relaxase intermediate for a MOBT relaxase.
Whole-genome long-read TAPS deciphers DNA methylation patterns at base resolution using PacBio SMRT sequencing technology
Authors: Chen Jinfeng , Cheng Jingfei , Chen Xiufei , Inoue Masato , Liu Yibin , Song Chun-Xiao 
Published: July 2022

Abstract
Long-read sequencing provides valuable information on difficult-to-map genomic regions, which can complement short-read sequencing to improve genome assembly, yet limited methods are available to accurately detect DNA methylation over long distances at a whole-genome scale. By combining our recently developed TET-assisted pyridine borane sequencing (TAPS) method, which enables direct detection of 5-methylcytosine and 5-hydroxymethylcytosine, with PacBio single-molecule real-time sequencing, we present here whole-genome long-read TAPS (wglrTAPS). To evaluate the performance of wglrTAPS, we applied it to mouse embryonic stem cells as a proof of concept, and an N50 read length of 3.5 kb is achieved. By sequencing wglrTAPS to 8.2× depth, we discovered a significant proportion of CpG sites that were not covered in previous 27.5× short-read TAPS. Our results demonstrate that wglrTAPS facilitates methylation profiling on problematic genomic regions with repetitive elements or structural variations, and also in an allelic manner, all of which are extremely difficult for short-read sequencing methods to resolve. This method therefore enhances applications of third-generation sequencing technologies for DNA epigenetics.
Depatuxizumab mafodotin in EGFR-amplified newly diagnosed glioblastoma: A phase III randomized clinical trial
Authors: Lassman Andrew B , Pugh Stephanie L , Wang Tony J C , Aldape Kenneth , Gan Hui K , Preusser Matthias , Vogelbaum Michael A , Sulman Erik P , Won Minhee , Zhang Peixin , Moazami Golnaz , Macsai Marian S , Gilbert Mark R , Bain Earle E , Blot Vincent , Ansell Peter J , Samanta Suvajit , Kundu Madan G , Armstrong Terri S , Wefel Jeffrey S , Seidel Clemens , de Vos Filip Y , Hsu Sigmund , Cardona Andrés F , Lombardi Giuseppe , Bentsion Dmitry , Peterson Richard A , Gedye Craig , Bourg Véronique , Wick Antje , Curran Walter J , Mehta Minesh P 
Published: July 2022

Abstract

Background
Approximately 50% of newly diagnosed glioblastomas (GBMs) harbor epidermal growth factor receptor gene amplification (EGFR-amp). Preclinical and early-phase clinical data suggested efficacy of depatuxizumab mafodotin (depatux-m), an antibody–drug conjugate comprised of a monoclonal antibody that binds activated EGFR (overexpressed wild-type and EGFRvIII-mutant) linked to a microtubule-inhibitor toxin in EGFR-amp GBMs.

Methods
In this phase III trial, adults with centrally confirmed, EGFR-amp newly diagnosed GBM were randomized 1:1 to radiotherapy, temozolomide, and depatux-m/placebo. Corneal epitheliopathy was treated with a combination of protocol-specified prophylactic and supportive measures. There was 85% power to detect a hazard ratio (HR) ≤0.75 for overall survival (OS) at a 2.5% 1-sided significance level (ie traditional two-sided p ≤ 0.05) by log-rank testing.

Results
There were 639 randomized patients (median age 60, range 22–84; 62% men). Prespecified interim analysis found no improvement in OS for depatux-m over placebo (median 18.9 vs. 18.7 months, HR 1.02, 95% CI 0.82–1.26, 1-sided p = 0.63). Progression-free survival was longer for depatux-m than placebo (median 8.0 vs. 6.3 months; HR 0.84, 95% confidence interval [CI] 0.70–1.01, p = 0.029), particularly among those with EGFRvIII-mutant (median 8.3 vs. 5.9 months, HR 0.72, 95% CI 0.56–0.93, 1-sided p = 0.002) or MGMT unmethylated (HR 0.77, 95% CI 0.61–0.97; 1-sided p = 0.012) tumors but without an OS improvement. Corneal epitheliopathy occurred in 94% of depatux-m-treated patients (61% grade 3–4), causing 12% to discontinue.

Conclusions
Interim analysis demonstrated no OS benefit for depatux-m in treating EGFR-amp newly diagnosed GBM. No new important safety risks were identified.
Implementation of Outpatient Infectious Diseases E-Consults at a Safety Net Healthcare System
Authors: Medford Richard J , Granger Madison , Pickering Madison , Lehmann Christoph U , Mayorga Christian , King Helen 
Published: July 2022

Abstract

Background
Safety net healthcare systems have high patient volumes and significant demands for specialty care including infectious diseases (ID) consultations. Electronic ID consults (E-consults) can lessen this burden by providing an alternative to face-to-face ID referrals and decreasing financial, time, and travel constraints on patients. This system could increase access to ID care for patients in limited-resource settings.

Methods
We described characteristics of all outpatient ID E-consults at Parkland Health in Dallas, Texas, from March 2018 to February 2021. We used modeling to determine which characteristics influenced conversion of E-consults to clinic visits and integrated these data into a predictive model for face-to-face conversion.

Results
For 725 E-consults, common E-consult topics included 118 (16%) latent tuberculosis, 116 (16%) syphilis, and 76 (10%) gastrointestinal infections. Nearly two-thirds of E-consults (456 [63%]) were requested by primary care providers. The majority (78%) were resolved without a face-to-face ID visit. Osteomyelitis, nontuberculous mycobacterial, and gastrointestinal questions frequently required face-to-face visits at rates of 65%, 49%, and 32%, respectively. Our logistic regression model predicted the need for a face-to-face visit with 80% accuracy and an area under the receiver operating characteristic curve of 0.72.

Conclusions
An outpatient ID E-consult program at a safety net healthcare system was an effective tool to provide timely input on common ID topics. E-consults were requested by a range of providers, and most were completed without a face-to-face visit. Predictive modeling identified important characteristics of E-consults and predicted conversion to face-to-face visits with reasonable accuracy.
JOINT SOGC-CPS CLINICAL PRACTICE GUIDELINE
Authors: McDonald Sarah D , Narvey Michael , Ehman William , Jain Venu , Cassell Krista 
Published: July 2022

Abstract

Objective
To assess the impact of deferred (delayed) cord clamping (DCC) and umbilical cord milking in singleton and twin gestations on maternal and infant mortality and morbidity.

Target Population
Women who are pregnant with preterm or term singletons or twins.

Benefits, Harms, and Costs
In preterm singletons, DCC for (ideally) 60 to 120 seconds, but at least for 30 seconds, reduces infant risk of mortality and morbidity. DCC in preterm twins is associated with some benefits. In term singletons, DCC for 60 seconds improves hematological parameters. In very preterm infants, umbilical cord milking increases risk for intraventricular hemorrhage.

Evidence
Searches of Medline, PubMed, Embase, and the Cochrane Library from inception to March 2020 were undertaken using Medical Subject Heading (MeSH) terms and key words related to deferred cord clamping and umbilical cord milking. This document represents an abstraction of the evidence rather than a methodological review.

Validation Methods
The authors rated the quality of evidence and strength of recommendations using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach. See online Appendix A (Tables A1 for definitions and A2 for interpretations of strong and conditional [weak] recommendations).
Zircon Reveals Diverse Trends of Magma Crystallization from Two Types of Early Post-Collisional Diorites (Variscan Orogen, NE Bohemian Massif)
Authors: Pietranik Anna , Farina Federico , Derkowska Katarzyna , Schaltegger Urs , Przybyło Arkadiusz , Storey Craig , Lasalle Stephanie , Dhuime Bruno , Pańczyk Magdalena , Zieliński Grzegorz , Nowak Małgorzata , Bulcewicz Kamil , Kierczak Jakub 
Published: July 2022

Abstract
Amphibole- and clinopyroxene-bearing monzodiorites were emplaced at 340 Ma (CA-ID-TIMS zircon age), suggesting the formation of hydrous and dry magmas closely related in space and time in the NE Bohemian Massif. Hafnium and oxygen isotopes of zircon in less evolved rocks (&lt;55 wt% SiO2) are similar between Amp and Cpx monzodiorites (εHf = −3.3 ± 0.5 and − 3.5 ± 0.8; δ18O = 6.4 ± 1.0 and 6.8 ± 0.7, respectively), consistent with a common source—a contaminated mafic magma derived from an enriched mantle. At the same time, the conditions of crystallization are distinct and zircon appears to be an excellent tool for distinguishing between hydrous and anhydrous crystallization conditions, a process that may be more ambiguously recorded by whole rock and major mineral chemistry. In particular, elements fractionated by either amphibole or plagioclase crystallization, such as Hf, Dy, and Eu, differ in zircon from amphibole- and clinopyroxene-bearing rocks, and Zr/Hf, Yb/Dy, and Eu/Dy are therefore useful indices of crystallization conditions. We show that the composition of zircon from hydrous dioritic magmas is not comparable with that of typical zircon from dioritic-granitic suites worldwide, suggesting a specific process involved in their formation. Here, we propose that fluid-present remelting of a mafic underplate is necessary to explain the rock textures as well as the composition of the whole rock, zircon, and other minerals of amphibole-bearing monzodiorites and that a similar process may control the formation of amphibole-rich dioritic rocks worldwide, including appinitic suites. Overall, we show that dioritic rocks represent snapshots of differentiation processes that occur in the early stages of magma evolution before the magma is homogenized into large-scale batholiths.
Bluefin Larvae in Oligotrophic Ocean Foodwebs, investigations of nutrients to zooplankton: overview of the BLOOFINZ-Gulf of Mexico program
Authors: Gerard Trika , Lamkin John T , Kelly Thomas B , Knapp Angela N , Laiz-Carrión RaÚl , Malca Estrella , Selph Karen E , Shiroza Akihiro , Shropshire Taylor A , Stukel Michael R , Swalethorp Rasmus , Yingling Natalia , Landry Michael R 
Published: July 2022

Abstract
Western Atlantic bluefin tuna (ABT) undertake long-distance migrations from rich feeding grounds in the North Atlantic to spawn in oligotrophic waters of the Gulf of Mexico (GoM). Stock recruitment is strongly affected by interannual variability in the physical features associated with ABT larvae, but the nutrient sources and food-web structure of preferred habitat, the edges of anticyclonic loop eddies, are unknown. Here, we describe the goals, physical context, design and major findings of an end-to-end process study conducted during peak ABT spawning in May 2017 and 2018. Mesoscale features in the oceanic GoM were surveyed for larvae, and five multi-day Lagrangian experiments measured hydrography and nutrients; plankton biomass and composition from bacteria to zooplankton and fish larvae; phytoplankton nutrient uptake, productivity and taxon-specific growth rates; micro- and mesozooplankton grazing; particle export; and ABT larval feeding and growth rates. We provide a general introduction to the BLOOFINZ-GoM project (Bluefin tuna Larvae in Oligotrophic Ocean Foodwebs, Investigation of Nitrogen to Zooplankton) and highlight the finding, based on backtracking of experimental waters to their positions weeks earlier, that lateral transport from the continental slope region may be more of a key determinant of available habitat utilized by larvae than eddy edges per se.
On the Strict Majorant Property in Arbitrary Dimensions
Authors: Gressman P T , Guo S , Pierce L B , Roos J , Yung P -L 
Published: July 2022

AbstractIn this work we study d-dimensional majorant properties. We prove that a set of frequencies in $\mathbb{Z}^d$ satisfies the strict majorant property on $L^p([0,1]^d)$ for all p &gt; 0 if and only if the set is affinely independent. We further construct three types of violations of the strict majorant property. Any set of at least d + 2 frequencies in $\mathbb{Z}^d$ violates the strict majorant property on $L^p([0,1]^d)$ for an open interval of $p \not\in 2\mathbb{N}$ of length 2. Any infinite set of frequencies in $\mathbb{Z}^d$ violates the strict majorant property on $L^p([0,1]^d)$ for an infinite sequence of open intervals of $p \not\in 2\mathbb{N}$ of length 2. Finally, given any p &gt; 0 with $p \not\in 2\mathbb{N}$, we exhibit a set of d + 2 frequencies on the moment curve in $\mathbb{R}^d$ that violate the strict majorant property on $L^p([0,1]^d).$
An Inter-Comparison of Passive Dosemeters for Terrestrial Gamma Radiation Measurements
Authors: Ding Yanqiu , Wang Kaiyi , Gao Pin , Hu Aiying , Guo Wen , Han Yanqing 
Published: July 2022

Abstract
To evaluate the measurement method of passive dosemeters for terrestrial gamma radiation measurements. An inter-comparison of passive dosemeters for terrestrial gamma radiation measurements was organized alternately by the Japan Chemical Analysis Center, National Institute for Radiological Protection of Chinese Center for Disease Control and Prevention and Radiation Monitoring Technical Center of Ministry of Environmental Protection every 2 years. The procedures and exposure conditions of the field-exposure and reference irradiation test were provided by the organizer. During 2019–2020, the En values of Field-1 and Field-2 in three laboratories were lower than 1.0 and assigned ‘acceptable’ according to inter-comparison criteria. After the correction of the fading effect of dosemeter material, the En values of reference irradiation test (0.301 mGy, 1.000 mGy) in three laboratories were lower than 1.0 and assigned ‘acceptable’.
Jumping to Conclusions and Its Associations With Psychotic Experiences in Preadolescent Children at Familial High Risk of Schizophrenia or Bipolar Disorder-The Danish High Risk and Resilience Study, VIA 11
Authors: Gregersen Maja , Rohd Sinnika Birkehøj , Jepsen Jens Richardt Møllegaard , Brandt Julie Marie , Søndergaard Anne , Hjorthøj Carsten , Knudsen Christina Bruun , Andreassen Anna Krogh , Veddum Lotte , Ohland Jessica , Wilms Martin , Krantz Mette Falkenberg , Burton Birgitte Klee , Greve Aja , Bliksted Vibeke , Mors Ole , Clemmensen Lars , Nordentoft Merete , Thorup Anne Amalie Elgaard , Hemager Nicoline 
Published: July 2022

Abstract

Background
The jumping to conclusions (JTC) bias, ie, making decisions based on inadequate evidence, is associated with psychosis in adults and is believed to underlie the formation of delusions. Knowledge on the early manifestations of JTC and its associations with psychotic experiences (PE) in children and adolescents is lacking.

Design
Preadolescent children (mean age 11.9 y, SD 0.2) at familial high risk of schizophrenia (FHR-SZ, n = 169) or bipolar disorder (FHR-BP, n = 101), and controls (n = 173) were assessed with the Beads Task to examine JTC. The number of beads drawn before making a decision, “draws to decision” (DTD) was used as a primary outcome. PE were ascertained in face-to-face interviews. General intelligence was measured with Reynolds Intellectual Screening Test.

Results
Children at FHR-SZ took fewer DTD than controls (4.9 vs 5.9, Cohen’s d = 0.31, P = .004). Differences were attenuated when adjusting for IQ (Cohen’s d = 0.24, P = .02). Higher IQ was associated with a higher number of DTD (B = 0.073, P &lt; .001). Current subclinical delusions compared with no PE were associated with fewer DTD in children at FHR-SZ (P = .04) and controls (P &lt; .05). Associations between delusions and DTD were nullified when accounting for IQ.

Conclusions
JTC marks familial risk of psychosis in preadolescence, not reducible to general intelligence. JTC is associated with subclinical delusions, but this may be an expression of intellectual impairment. Future studies should establish temporality between JTC and delusion formation and examine JTC as a target for early intervention.
Bling-Bling politics: exposure to status-goods consumption shapes the social policy preferences of the less affuent
Authors: Lungu Laura Silvia 
Published: July 2022

Abstract
Consumer behavior and sociological research have recognized early on the negative externalities of exposure to conspicuous consumption: anxiety, debt and wasteful consumption. This article contributes to political economy by incorporating the costs of exposure to wealth-signaling consumption into the materialist self-interest model of social policy preferences. The argument is that exposure to conspicuous consumption reduces support for social spending and increases demand for lower income taxes. Tax cuts impact purchasing power directly, allowing individuals to keep up with consumption standards and to avoid looking poor by comparison. Two USA-based analyses using fine-grained data on consumption and individual attitudes at the zip code and county level support the argument. Importantly, less-affluent citizens are more likely to prioritize cuts to social spending over tax increases, at higher levels of conspicuous consumption. Additional analyses rule out alternative explanations like upward mobility prospects, local wealth effects and partisan context.
Ups and downs in finance, ups without downs in inequality
Authors: Godechot Olivier , Neumann Nils , Apascaritei Paula , Boza István , Hallsten Martin , Henriksen Lasse , Hermansen Are , Hou Feng , Jung Jiwook , Kodama Naomi , Křížková Alena , Lippényi Zoltán , Elvira Marta M , Melzer Silvia Maja , Mun Eunmi , Sabanci Halil , Soener Matthew , Thaning Max 
Published: July 2022

Abstract
The upswing in finance in recent decades has led to rising inequality, but do downswings in finance lead to a symmetric decline in inequality? We analyze the asymmetry of the effect of ups and downs in finance, and the effect of increased capital requirements and the bonus cap on national earnings inequality. We use administrative employer–employee-linked data from 1990 to 2019 for 12 countries and data from bank reports, from 2009 to 2017 in 13 European countries. We find a strong asymmetry in the effect of upswings and downswings in finance on earnings inequality, a weak, if any, mitigating effect of capital requirements on finance’s contribution to inequality, and a restructuring but no absolute effect of the bonus cap on financiers’ earnings. We suggest that while rising financiers’ wages increase inequality in upswings, they are resilient in downswings and thus downswings do not contribute to a symmetric decline in inequality.
‘Malaria Has Spoilt It’: Malaria, Neuropsychiatric Complications, and Insanity in ex-Servicemen in Post-First World War Britain
Authors: Fantauzzo Justin 
Published: July 2022

Summary
This article focuses on the cases of two British ex-servicemen who contracted malaria during or immediately after the First World War, were charged with murder in the 1920s, and pled insanity due to their malaria and long-term neuropsychiatric complications. One was found ‘guilty but insane’ and committed to Broadmoor Criminal Lunatic Asylum in June 1923, while the other was convicted and hanged in July 1927. It argues that, at a time when the medical community sought out the causes of mental disease in the physical body, medico-legal arguments about malaria and insanity were received inconsistently by inter-war British courts. Class, education, social status, institutional support and the nature of the crime all mattered, as they had in the diagnoses, treatment and trials of other ex-servicemen with psychiatric illnesses.
“We Got Witnesses” Black Women’s Counter-Surveillance for Navigating Police Violence and Legal Estrangement
Authors: Gonzalez Shannon Malone , Deckard Faith M 
Published: July 2022

Abstract
Police violence shapes the lives of racial and ethnic minorities, and while much has been written about strategic responses to police, missing is an examination of how black women navigate interactions with officers. Based on 32 interviews with black women, we find that they use witnessing, or the mobilization of others as observers to police encounters. Research demonstrates the rising role of videos and smartphones in documenting encounters with officers. We find that black women adapt witnessing techniques based on their surroundings, available resources, and network contacts. Three forms of witnessing are observed: physical witnessing, mobilizing others in close proximity to interactions with officers; virtual witnessing, using cellphone or social media technology to contact others or record interactions with officers; and institutional witnessing, leveraging police or other institutional contacts as interveners to interactions with officers. Black women mobilize witnessing to deescalate violence, gather evidence, and promote accountability. Attuned to both the interactional and structural dynamics of police encounters, black women conceptualize witnessing as a way to survive police encounters and navigate their legal estrangement within the carceral system. We theorize black women’s witnessing as a form of resistance as they work to reconfigure short- and long-term power relations between themselves, their communities, and police.