bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urine Myoglobin is Associated with Different Severities and Depths for Burn Patients
Authors: Li Tao , Zhao Qiaomei , Wang Jitu , Liu Tianci , Zhang Man 
Published: February 2022

Abstract
Backgrounds It is important to make reasonable clinical decision based on precise burn depth for burn specialists. However, the identification of burn depth based upon clinical assessment was not accurate. It still needs more biomarkers to rapidly distinguish different depths for burn specialists. The potential value of urine myoglobin (UM) is still unknown in burn patients. Patients and methods The urine of 30 burn patients and 10 Healthy volunteers (Discover set) were collected from January 2017 to December 2020. Urine proteomics of all samples was identified by Label-free mass spectrometry and functional analysis for differential expression proteins (DEPs) was also analyzed. The Receiver Operating Characteristic curve to evaluate the value of UM from another 67 burn patients (Validation set). Results A total of 8253 peptides and 1465 proteins were identified in four groups. For severe burn patients, 402 DEPs (279 up-regulated and 123 down-regulated) were identified and functional analysis demonstrated that neutrophil-mediated immunity, response to stimulus, and complement and coagulation cascades pathway were the most enrichment function. UM had significant differences in burn patients with different severities and depths (Discover and Validation set; P<0.05). UM as a novel biomarker could rapidly distinguish different depths for burn patients (Discover set, AUC=0.8203, 95%CI: 0.6448~0.9957; Validation set, AUC=0.7393, 95%CI: 0.618~0.8606). Conclusion The urine DEPs were mainly identified in the severe group. UM might as a novel biomarker help burn specialists to rapidly determine different depths for burn patients.
Acetylation of ATP5f1c mediates cardiomyocyte senescence via metabolic dysfunction in radiation induced heart damage
Authors: Zeng Zhimin , Xu Peng , He Yanqing , Yi Yali , Liu Zhicheng , Cai Jing , Huang Long , Liu Anwen 
Published: February 2022

Abstract
Objective: Ionizing radiation (IR) causes heart senescence, which eventually manifests as radiation-induced heart damage (RIHD). Energy metabolism of cardiomyocytes is involved in senescence. This study attempts to explore radiation-induced senescence mechanisms through TMT-labeled proteomics.Methods: Echocardiography, senescence-associated secretory phenotype (SASP), telomere length and P21 expression were conducted to detect IR induced senescence. Total protein acetylation of irradiated heart apex and H9C2 cells was discovered by Western blotting compared with the control. To further investigate lysine acetylation alterations in irradiated heart proteins, proteomics of lysine acetylation was performed. Acetylated level of Atp5f1c was verified by Co-IP. The plasmids of the acetylated-mimicking Atp5f1c mutant (K55Q), deacetylated-mimicking Atp5f1c mutant (K55R) and WT were constructed and used for to transfect H9C2 cells to prove that acetylated Atp5f1c participated in energy metabolism and senescence. Deacetylation inhibitors, plasmid transfection and Co-IP were used to determine the mechanism of upstream regulation of Atp5f1c K55-Ac.Results: LVEF was decreased, SASP was elevated, telomere length was shortened and P21was overexpressed after IR. In addition, the total protein acetylation level significantly increased, and 69 proteins, including 90 sites, showed a lysine acetylation response to irradiation. The majority of the hyperacetylated proteins were related to energy metabolism. The ATP synthase γ subunit was hyperacetylated at the 55th lysine site and led to decreased ATP enzyme activity and synthesis decreased. Atp5f1c K55-Ac induced H9C2 metabolic dysfunction and senescence. Sirt4 and Sitr5, deacetylase inhibitors, mediated Atp5f1c K55-Ac deacetylation. Conclusion: IR induced cardiomyocyte senescence and energy metabolism disorder. Atp5f1c K55-ac, which is modulated by Sirt4 or Sirt5, might be a potential molecular target underlying RIHD.
Modelling can reduce contamination from mosquito population control
Authors: Djerdj Tamara , Hackenberger Domagoj K. , Klanjšček Tin , Hackenberger Branimir K. 
Published: February 2022

Abstract
Environmental contamination due to pest control in general, and mosquito control in particular, is an important issue expected to increase with climate change. We use a validated model for population dynamics of mosquitoes and historical environmental data to explore performance of larvicidal, adulticidal, and combined treatments. Results show that depending on treatment timing, larvicidal treatments can induce very good results, or have negative outcomes that increase overall mosquito population. Combined larvicidal and adulticidal treatments, however, exhibit much lesser dependence on timing, and therefore give the greatest chance of positive outcomes if environmental conditions are not known. Based on the results, we argue for adaptive mosquito management, in which weather data and forecasts are used to drive a model that identifies best intervals for insecticide use. Such an approach can have considerably better results than static, calendar-driven management and, therefore, considerably reduce environmental contamination. Adaptive management could consider larvicidal treatment because it gives good results if the timing is correct. Static management should, however, combine larvicidal and adulticidal treatments for the greatest chance of success.
Multi-omics Reveal the Enabling Factors of Aplastic Anaemia
Authors: Zhao Xiaoyu , Wang Chaomeng , Liu Chenchen , Jiang Nan , Ding Shaoxue , Chen Yingying , Yang Liping , Liu Chunyan , Fu Rong 
Published: February 2022

Abstract
Background: Aplastic anemia is a kind of anemia caused by bone marrow failure due to autoimmune abnormalities. Numerous studies have shown that autoimmunity is regulated by intestinal microflora, and that most intestinal microflora regulates immunity through short chain fatty acids. At the same time, almost all patients with aplastic anemia will have bone marrow adipose tissue, and the process of bone marrow adipose tissue is regulated by medium and long chain fatty acids. Our previous studies have found that the intestinal flora of aplastic anemia patients is different from that of normal people. Therefore, this study aims to conduct a comprehensive detection of the intestinal flora and fatty acid metabolism in patients with aplastic anemia and to study their correlation.Methods: Fatty acid compositions in the peripheral plasma and bone marrow supernatants of 12 newly diagnosed aplastic anaemia patients and 10 normal controls were comprehensively analysed by CG-MS. Gene sequencing of faecal samples from both groups was also performed with macrogene sequencing technology.Results: Based on the metagenomic sequencing technology, we analyzed the difference of intestinal flora between group AA and group NC. We found that the main difference between the two groups existed in the bacterial community, and the abundance of most microbial species showed a downward trend. Based on the CG-MS lipid detection technique, we found that there were differences in lipid metabolism between the AA group and the NC group, whether short chain fatty acids or medium and long chain fatty acids. 8. The most attractive findings are interrelationships between the Citrobacter spp 、stearic acid (c18:0), isobutyric acid, and lysine degradation and betaine biosynthesis pathway and the interrelationship between catenibacterium_mitsuokai species at Catenibacterium, cis-7,10,13,16-docosatetraenoic acid (c22:4), ubiquinone and other terpenoid quinones pathway.We also found the intercorrelation between the abundance of Enhydrobacter genus and Enhydrobacter_aerosaccus species, cis-13,16-docosadienoic acid in peripheral plasma, cis-7,10,13,16-docosatetraenoic acid in bone marrow supernatant, tyrosine and tryptophan biosynthesis pathways.Conclusions: Our results show that Citrobacter infection may be a driving factor for aplastic anaemia, identifying a potential role for stearic acid in the immunopathogenesis of aplastic anaemia. Our study also demonstrates the potential role of 22-carbon long-chain polyunsaturated fatty acids, which may not only be involved in the metabolism of fibroblasts in bone marrow but also influence the formation of the bone marrow microenvironment, in aplastic anaemia.
Short measures of youth psychopathology: Psychometric properties of the Brief Problem Monitor (BPM) and Behavior and Feelings Survey (BFS) in a Norwegian clinical sample
Authors: Rognstad Kristian , Helland Siri Saugstad , Neumer Simon-Peter , Baardstu Silje , Kjøbli John 
Published: February 2022

Abstract
Background: Tracking clinical outcomes during therapy can be useful for improving both clinical practice and research. For repeated data collection, short, reliable, and valid measures of central aspects of psychopathology are necessary. The current paper investigates the psychometric properties of two short surveys for measuring central dimensions of psychopathology in youth. Methods: We investigated factor structure and validity of the Norwegian translations of the Behavior and Feelings Survey (BFS) and the Brief Problem Monitor (BPM). The BFS has previously shown a two-factor structure and indications of validity as a measure of internalizing and externalizing problems in youth. Likewise, the BPM has support for a three-factor structure of internalizing, externalizing and attention problems. In our sample of 503 youths and caregivers in a Norwegian outpatient clinic, we conducted confirmatory factor analyses to test the assumed measurement models and further considered concurrent validity of the measures. Results: The results suggest that the assumed measurement models for both questionnaires only partly fit our data but that subscales of the BFS and BPM still indicate convergent validity. Conclusions: Alternative measurement models, and the usefulness and limitations of these short form questionnaires for internalizing and externalizing problems, are discussed.
ct-DNA is useful to diagnose mutations at codon 641 of exon 16 of EZH2, which is a biomarker for relapse in patients with Diffuse Large B-cell Lymphoma treated with the R-CHOP scheme.
Authors: Díaz-Chavez Jose , Gutiérrez-Hernández Olga , Taja-Chayeb Lucia , Gutiérrez-Chavarría Sindy , Aviles-Salas Alejandro , Candelaria Myrna 
Published: February 2022

Abstract
Background: The epigenetic regulator EZH2 is a subunit of the polycomb repressive complex 2 (PRC2), methylates H3K27, resulting in transcriptional silencing. The mutation at Y646 amino acid in the EZH2 gene is mutated in up to 40 % of B-cell lymphomas. We compared the presence of exon 16 EZH2 mutations in tumor samples and ctDNA in a prospective trial. The mutations were determined by sanger sequencing, and by ddPCR. We also evaluated the impact of these mutations on response, relapse, and survival. Results: One hundred and thirty-eight cases were included. Ninety-eight were germinal center, and twenty had EZH2 mutations. Mean follow-up (IQR 25-75) was 23 (7- 42) months. The tumor samples were considered the standard of reference. Considering the results of the mutation in ctDNA by Sanger sequencing, the sensibility (Se) and specificity (Sp) were 52 % and 99 %, respectively. After adding the droplet digital PCR (ddPCR) analysis, the Se and Sp increased to 95 and 100 %, respectively. After bivariate analysis, only the presence of double-hit lymphoma (p=0.04), or EZH2 mutations were associated with relapse. The median PFS (95 % Interval confidence) was 27.7 (95 % IC: 14-40) vs 44.1 (95 % IC: 40-47.6) months for the mutated vs wt patients. Conclusions: The ctDNA is usefull to analyse EZH2 mutations, which have an impact in PFS.
Pretreatment neutrophil to lymphocyte ratio as prognostic factor in metastatic breast cancer treated with cyclin dependent kinase 4/6 inhibitors
Authors: Rottier Pauline , SILVA Angélique DA , JOHNSON Alison , LEVY Christelle , ALLOUACHE Djelila , HRAB Ioana , SEGURA Carine , MOREL Adeline , VILLEMIN Maud , BOSCHER Clémence , BERTHO Marion , BOISMOREAU Louis , CHERIFI François , LEQUESNE Justine , EMILE George 
Published: February 2022

Abstract
Background: Cyclin dependent kinase inhibitors (CdK4/6i) changed the course of hormone receptor positive (HR+) HER2negative (HER2-) metastatic breast cancer (mBC). To date, no factors have been shown to predict response to CdK4/6i. Neutrophil-to-lymphocyte ratio (NLR), an indicator of the host systemic inflammatory response, is an independent prognostic factor for survival in cancers. We conducted this study to evaluate the addition of NLR for survival assessment in mBC patients treated with first line CdK4/6i. Methods: All mBC patients treated with first line CdK4/6i between November 2015 and December 2019 were retrospectively included. We analyzed 12-month progression free survival (PFS), overall survival (OS) and response rate according to NLR in univariable and multivariable analysis.Results: A total of 126 patients treated with palbociclib (n=101), ribociclib (n=18) or abemaciclib (n=7) were included, with a median follow-up of 16 months [range: 2.9-37]. Median age was 65 years [29-86], 88% patients had good performance status (0-1). Most patients were included at the metastatic relapse stage (71%) and 20% had visceral metastases without bone lesions. High NLR (≥ 2.53) was significantly associated with worse PFS (Hazard Ratio (HR)=0.52, CI95% =[0.29-0.94]). Fourteen patients died (11.1%). Complete responses were more frequent in the low NLR group (<2.53) (14.5% vs. 3.1%, p=0.029). Conclusion: High NLR is associated with worse PFS in HR+ HER2- mBC patients treated with first line CdK4/6i. NLR is a reliable and inexpensive prognostic marker, easily accessible in routine clinical practice, which could optimize the therapeutic strategy. These results need to be confirmed in larger prospective studies.
Impact of the COVID19-related economic crisis and mitigation effects of social protection on HIV/AIDS and Tuberculosis: an integrated economic, mathematical and epidemiological modelling study
Authors: Rubio Felipe , Amad Alan , Moya Erick , de Oliveira Robson , Aransiola Temidayo , Anderle Rodrigo , Sironi Alberto , Ordoñez José , Sanchez Mauro , de Oliveira Juliane , de Souza Luis Eugenio , Dourado Inês , Macinko James , Rasella Davide 
Published: February 2022

Abstract
The COVID-19 pandemic will have impactful long-term effects on the global rise of poverty and social inequalities, potentially compromising the achievement of SDGs for poverty-related diseases. We aimed to evaluate the impact of poverty increases, and the mitigation effects of social protection policies, on HIV/AIDS and Tuberculosis (TB) in Brazil, one of the largest and most unequal LMICs. We integrated economic, mathematical, and epidemiological models to forecast the trends of HIV/AIDS and TB according to different future scenarios, showing that the implementation of social protection policies could mitigate long-lasting rises in poverty, avoiding -in the most pessimistic economic projections- an increase of 41% in the incidence and 50% in the mortality from HIV/AIDS, and of 32% in the incidence and 53% in the mortality from TB. Overall, more than 250 thousand cases and 43 thousand deaths from HIV/AIDS and TB could be averted up to 2030.
Evaluated the Role of N6-Methyladenosine-associated lncRNAs in Prognosis and Immune Microenvironment of Colorectal Cancer
Authors: Yuan Congfei , Liu Caidong , Zhao Shuli , Zhang Xishan , Jia Haifeng , Chen Baiyu , Zhang Maojin , Zheng Yuan , Wei Xiaowei , Zhou Jin , Bo Yanzhi 
Published: February 2022

Abstract
Background: The role of N6-methyladenosine medicated long non-coding RNAs (lncRNAs) is elusive in colorectal cancer (CRC). Materials and Methods: We identified m6A-associated lncRNAs by using the data gathered from The Cancer Genome Atlas (TCGA) and stratified CRC patients into different subgroups. Cox-regression analysis were performed to construct a m6A-associated lncRNA signature. And the role of this signature in prognosis and immune microenvironment was dissected subsequently. Finally, a gene set enrichment analysis (GSEA) were executed to predict the possible bio-mechanisms on the basis of the signature.Results: Three m6A-associated clusters were constructed from 866 differentially expressed lncRNAs. Cluster 2 had poor prognosis and low immune cell infiltration. A m6A-associated lncRNA signature consisting of 14 lncRNAs was constructed, and recognized as an independent prognostic indicator in CRC by using survival analysis and receiver operating characteristic (ROC) curves. The clinical features and immune cell infiltration status were significantly different in the patients stratified by risk score. Furthermore, GSEA showed that P53 pathway and Natural killer cell mediated cytotoxicity were more enriched in the low risk group.Conclusion: Our data revealed that m6A-associated lncRNAs could be potential prognostic and immunogenicity indicators in CRC.
Imagined Head Rotations Facilitate Spatial Memory
Authors: Wyssen Gerda , Mast Fred W. , Ertl Matthias 
Published: February 2022

Abstract
Neuroimaging studies have shown great similarities between the cortical networks involved in motor execution and motor imagery. However, none of those studies report data on head or full body motions and only few provide evidence of behavioral consequences of this similarity. Here, we report results from a novel kinesthetic spatial memory task, in which participants encoded a target position by an active, passive or imagined head rotation. During the experiment, participants were seated on a motion platform with a VR headset, which allowed passive motion and head tracking. To compare all three encoding conditions, precision and head velocity in the executions of head movements were analyzed with Bayesian hierarchical generalized linear regression models. We found comparable performance in precision in the active and imagery conditions whereas performance in the passive condition was significantly reduced. Head motion profiles were similar after active and imagined encoding. The results suggest that imagined head rotations provide useful sensorimotor information despite the absence of sensory and motor information during encoding. This can be explained by corollary discharge which is necessary for appropriate motor behavior and is likely also generated when the motor behavior is imagined but not executed.
COVID-19 in Tunisia (North Africa): Seroprevalence of SARS-CoV-2 in the general population of the capital city Tunis
Authors: Cherif Ines , Kharroubi Ghassen , Chaabane Sana , Yazidi Rihab , Dellagi Mongi , Snoussi Mohamed Ali , Salem Sadok , Marzouki Soumaya , Rebai Wafa Kammoun , Rourou Samia , Dellagi Koussay , Barbouche Mohamed Ridha , Benabdessalem Chaouki , Ahmed Melika Ben , Bettaieb Jihene 
Published: February 2022

Abstract

Background:Monitoring the coronavirus disease-19 (COVID-19) pandemic is primarily based on Reverse transcription polymerase chain reaction (RT PCR) detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). As this test is mainly applied on persons with symptomatic disease, it may overlook individuals with pauci- or a-symptomatic infection. Seroprevalence studies are thus essential to get an accurate estimate of the actual SARS-CoV-2 diffusion within the populations. We report on the findings of the first serosurvey conducted in the capital city of Tunisia prior to the implementation of mass vaccination and analyzed factors associated with seropositivity. Methods:A cross sectional survey was conducted among households living in two areas of the governorate of Tunis, the capital city of the country. The survey was realized between March and April 2021, spanning the end of the second wave and the beginning of the third wave of COVID-19 and prior to the implementation of mass vaccination in Tunisia. SARS-CoV-2 specific immunoglobulin G (IgG) antibodies to the spike (S-RBD) or the nucleocapsid (N) proteins were detected using an in-house ELISA test. Results were adjusted for test performance. Multivariate logistic regression analysis was performed to determine factors independently associated with SARS-COV-2 seropositivity.Results:The survey included 1676 individuals from 431 households. The mean age and sex ratio were 43.3±20.9 years and 0.6 respectively. The weighted and test-performance adjusted prevalence of IgG antibodies to the N and the S-RBD proteins were 26.6% [22.9-30.8] and 25.1% [22.2-28.4] respectively. The weighted seroprevalence of anti-N and/or anti-S-RBD IgG antibodies was equal to 38.0% [34.6-41.5]. In multivariate analysis, age under 10, no tobacco use, previous diagnosis of COVID-19, a history of COVID-19 related symptoms and contact with a COVID-19 case within the household, were independently associated with higher SARS-CoV-2 seroprevalence. Conclusions:After the end of the second epidemic wave, more than one third of people living in Tunis got antibodies to SARS-CoV-2. Further studies are needed to monitor changes in these figures as Tunisian population is confronted to the subsequent epidemic waves and to guide the vaccine strategy.
Differential Microbial Signature Associated With Benign Prostatic Hyperplasia and Prostate Cancer
Authors: Sarkar Purandar , Malik Samaresh , Banerjee Anwesha , Datta Chhanda , Pal Dilip Kumar , Ghosh Amlan , Saha Abhik 
Published: February 2022

Abstract
Apart from other risk factors, chronic inflammation is also associated with the onset of Prostate Cancer (PCa), wherein pathogen infection and tissue microbiome dysbiosis are known to play a major role for both inflammatory response and cancer development. However, except for a few studies, the link between microbes and PCa remained poorly understood. To explore the potential microbiome signature associated with PCa in Indian patients, we investigated differential compositions of commensal bacteria among patients with benign prostatic hyperplasia (BPH) and PCa using 16S rRNA amplicon sequencing followed by qPCR analyses using two distinct primer sets. Using two independent cohorts, we show that Prevotella copri, Cupriavidus campinensis and Propionibacterium acnes represent three most abundant bacteria in diseased prostate lesions. LEfSe analyses identified that while Cupriavidus taiwanensis and Methylobacterium organophilum are distinctly elevated in PCa samples, Kocuria palustris and Cellvibrio mixtus are significantly enriched in BPH samples. Furthermore, we identify that a number of human tumor viruses including Epstein-Barr virus (EBV), hepatitis B virus (HBV) along with two high risk human papillomaviruses - HPV-16 and HPV-18 are significantly associated with the PCa development and strongly correlated with PCa bacterial signature. The study may thus offer to develop a framework for exploiting this microbial signature for early diagnosis and prognosis of PCa development.
A Proposal of Two-step Autoregressive Model
Authors: Nguyen Loc 
Published: February 2022

Abstract
Autoregressive (AR) model and conditional autoregressive (CAR) model are specific regressive models in which independent variables and dependent variable imply the same object. They are powerful statistical tools to predict values based on correlation of time domain and space domain, which are useful in epidemiology analysis. In this research, I combine them by the simple way in which AR and CAR is estimated in two separate steps so as to cover time domain and space domain in spatial-temporal data analysis. Moreover, I integrate logistic model into CAR model, which aims to improve competence of autoregressive models.