bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of Whole Mount RNA multiplexed in situ Hybridization Chain Reaction with Immunohistochemistry, Clearing and Imaging to visualize octopus neurogenesis
Authors: Elagoz Ali M , Styfhals Ruth , Maccuro Sofia , Masin Luca , Moons Lieve , Seuntjens Eve 
Published: February 2022

AbstractGene expression analysis has been instrumental to understand the function of key factors during embryonic development of many species. Marker analysis is also used as a tool to investigate organ functioning and disease progression. As these processes happen in three dimensions, the development of technologies that enable detection of gene expression in the whole organ or embryo is essential. Here, we describe an optimized protocol of whole mount multiplexed RNA in situ hybridization chain reaction version 3.0 (HCR v3.0) in combination with immunohistochemistry (IHC), followed by fructose-glycerol clearing and light sheet fluorescence microscopy (LSFM) imaging on whole-mount Octopus vulgaris embryos. We developed a code to automate probe design which can be applied for designing HCR v3.0 type probe pairs for fluorescent in situ mRNA visualization. As proof of concept, neuronal (Ov-elav) and glial (Ov-apolpp) markers were used for multiplexed HCR v3.0. Neural progenitor (Ov-ascl1) and precursor (Ov-neuroD) markers were combined with an immunostaining for phosphorylated-histone H3, a marker for mitosis. After comparing several tissue clearing methods, fructose-glycerol clearing was found optimal in preserving the fluorescent signal of HCR v3.0. The expression that was observed in whole-mount octopus embryos matched with the previous expression data gathered from paraffin-embedded transverse sections. Three-dimensional reconstruction revealed additional spatial organization that had not been discovered using two-dimensional methods.
Tribological behavior of laser textured rolling element bearings under starved lubrication
Authors: Long Risheng , Shang Qingyu , Jin Zhihao , Zhang Yimin , Ju Zichen , Li Manhong 
Published: February 2022

Purpose
The purpose of this paper is to reveal the influence of dimples on the tribological properties of the “washers-cage-rollers” system of rolling element bearing (REB) under starved lubrication.

Design/methodology/approach
A fiber laser marking system was used to prepare dimples patterns on the raceways of the shaft washers of cylindrical roller thrust bearings (CRTBs). The friction and wear properties of dimples textured CRTBs with different diameter of dimples (200 µm, 250 µm, 300 µm) and depth of dimples (4 µm, 8 µm, 12 µm) were researched through a vertical universal wear test rig using a customized tribo-pair under starved lubrication. The surface stresses and the influence mechanism of dimple units on the tribological behavior of REBs were also compared and discussed.

Findings
Although the surface stresses on the raceways of dimples textured bearings are significantly higher than that of the smooth group, the coefficients of friction and wear losses of them are all reduced under starved lubrication. When the effective volume of dimples is between 1.13 and 2.25 and the depth of dimples is smaller than 12 µm, the tribological properties of dimples textured CRTBs are significantly improved. In this work, when the diameter of dimples is 250 µm, and the depth of dimples is 8 µm, the textured bearings can provide the excellent friction-reduction ability and outstanding wear resistance.

Originality/value
This work is a valuable reference for the raceway design and optimization of REBs.
Knowledge-based HR practices, π-shaped skills and innovative performance in the contemporary organizations
Authors: Elayan Malek Bakheet , Hayajneh Jamal Abdelrahman M. , Abdellatif Mamdouh Abdallah Mohamed , Abubakar A. Mohammed 
Published: February 2022

PurposeAs technology accelerates, the pace of innovation and product and process life cycles have significantly decreased. Firms seek to leverage their employees' efforts, particularly through knowledge-based HR practices and intellectual skills, to attain innovative performances. Despite extensive research, the scope and role of p-shaped skills have yet to be considered; this is an important oversight. Through the lens of a knowledge-based perspective, this study examines the association of knowledge-based HR practices, p-shaped skills and innovative performance.Design/methodology/approachThis paper tested the hypotheses using data obtained from large and SMEs in Riyadh, Saudi. A partial least squares structural equation modeling (PLS-SEM) technique was applied to analyze the data.FindingsThe results of the PLS-SEM algorithm suggest that knowledge-based HR practices result in increased p-shaped skills, and p-shaped skills result in increased innovative performance. Accordingly, p-shaped skills mediate the association between knowledge-based HR practices and innovative performances.Originality/valueThis is among the first study to operationalize a p-shaped skills scale. The paper extends its functionality as a predictor for innovative performance and the outcome of knowledge-driven HR practices. This research offers valuable theoretical and practical implications.
Sentiment analysis of online discussion of LIS professionals using R
Authors: Garg Mohit , Kanjilal Uma 
Published: February 2022

Purpose
The main focus of the present study was threefold. Firstly, extraction of the data from the Library and Information Science (LIS) Links; secondly, pre-process the data to remove noises on maximum parameters; and lastly, to know the polarity of the discussion posted on LIS Links.

Design/methodology/approach
The advancement in the internet and Web 2.0 technologies have facilitated the academic community with many online platforms like Q&A sites, mailing lists, discussion forums, etc. for disseminating or seeking information. LIS Links is one such discussion forum commonly used by Indian LIS professionals. The analysis of these discussions can help in knowing the experience of LIS professionals in different aspects. The present study is based on a lexicon-based approach, which works on the bag of word model. The open source environment R and its different packages were used for the analysis.

Findings
The analysis shows that the majority of the posts on LIS Links were discussed with positive sentiments. There were only very few words of negative sentiments on the discussion forum of LIS Links.

Originality/value
The above review of the literature shows that there have been many studies in different domains. The user responses on different platforms were explored. Also, some studies have conducted sentiment analysis in LIS. However, this was limited to content posted by the libraries or the reviews of books. No research was found in the published literature on the analysis of the opinion of LIS professional.
Environmental awards and their relevance to the environmental commitment of organizations
Authors: Conke Leonardo Silveira 
Published: February 2022

Purpose
The purpose of this paper is to identify the characteristics of environmental awards and analyze their relevance to the environmental commitment of organizations.

Design/methodology/approach
Extensive desk research, using secondary data and multiple documental sources on the environmental awards offered in Brazil.

Findings
Concerning their characteristics, awards are voluntary, do not have a standard set of guiding principles or regulations, can be offered by any desiring entity (accredited or not) and usually have qualitative evaluation methods. Regarding the contributions to social responsibility, organizations have been using awards to increase the visibility of their environmental efforts, reinforce their environmental image and disseminate environmental values and behaviors, especially within specific groups or communities. Consequently, it is argued why awards’ promotion or winning can be considered additional evidence of environmental commitment but should not be taken as a definite measure of environmental performance.

Originality/value
This paper provides a new perspective about awards, understanding them as types of recognition, and therefore, comparable to certification and labeling. Another contribution is gathering of awards’ characteristics, which can help analyze awards across several other fields, such as social responsibility, quality, innovation and public services.
The state as a whiteman, the whiteman as a/’hun: personhood, recognition, and the politics of knowability in the Kalahari
Authors: Ninkova Velina 
Published: February 2022

AbstractThe article is dedicated to the loving memory of !A|’xuni.The Ju|’hoansi of east central Namibia sometimes refer to the state as a whiteman and to the whiteman as a/’hun(steenbok). In this article, I contextualize these naming practices by tracing the history of colonial encounters on the fringes of the Western Kalahari through a small‐scale animist perspective. I then discuss what this means for the concept of ‘recognition’, which I treat as a two‐way intersubjective process of making oneself un/knowable to others. I argue that the Ju|’hoansi have engaged in parallel processes of mis/recognition vis‐à‐vis their colonial Others. By failing to enter into reciprocal relations with the Ju|’hoansi, the whiteman and the state have remained outside of the Ju|’hoansi's social universe and have thus compromised their own personhood.
‘Access to Women’: Securing Mormon Whiteness in the US Army's Regulation of Commercial Sex in Chihuahua, 1916
Authors: Maddock Pamela Jean 
Published: February 2022

AbstractThis article argues that Mormon colonists – once refugees who had sought freedom from persecution for their sexual practices – asserted a white middle‐class respectability as they cooperated with the US Army and corresponded with officers on the management of the soldiers’ sexual conduct. Their success depended heavily on shared understandings of the race and gender of the people involved. That is, they leveraged prevailing assumptions about Black soldiers’ bodies as aggressive and in need of sex, about white Mormon women's bodies as vulnerable and about Mexican women's bodies as racially in‐between and thus suitable for the sexual service‐work of enlisted men, pliable and ready to be made ‘accessible’ to soldiers. John Pershing, when asked to explain his decision to build the brothel, justified his choice by saying he had all Black troops at the camp and that nearby Mormon colonists had complained of these Black men meeting women outside for sex. This article explains how, why, for whom and to what end Pershing's explanation worked.
Compendium of sodium reduction strategies in foods: A scoping review
Authors: Dunteman Aubrey N. , McKenzie Elle N. , Yang Ying , Lee Youngsoo , Lee Soo‐Yeun 
Published: February 2022

AbstractIn response to health concerns generated by increased sodium intake, many new approaches have been studied to reduce the sodium content in processed food. It has been suggested that reducing sodium in the food supply may be the most appropriate solution. The aim of this scoping review was to establish what sodium reduction strategies are effective in maintaining acceptable sensory qualities for various food industry applications. Studies that evaluate and report on the effectiveness of a sodium reduction strategy relevant to food and included outcomes detailing how the strategies were received by human subjects using sensory data are included, as well as book chapters, literature reviews, and patents focusing on sodium reduction strategies. Only those published in English and since 1970 were included. Literature was obtained through Scopus, PubMed, EBSCOhost, and ScienceDirect databases, whereas patents were obtained through US Patent Trademark Office, Google Patents, and PATENTSCOPE databases. Two‐hundred and seventy‐seven primary studies, 27 literature reviews, 10 book chapters, and 143 patents were selected for inclusion. Data extracted included details such as analytical methods, broad and specific treatment categories, significant outcomes, and limitations among other material. Sodium reduction methods were categorized as either salt removal, salt replacement, flavor modification, functional modification, or physical modification. Although salt removal and salt replacement were the majority of included studies, future research would benefit from combining methods from other categories while investigating the impact on sensory characteristics, technological aspects, and consumer perception of the strategy.
Efficacy and safety of S‐flurbiprofen plaster in knee osteoarthritis patients: A 2‐week randomized controlled Phase III clinical trial compared to diclofenac gel
Authors: Tomatsu Kenji , Yasuda Shoji , Fuady Ahmad , Matsumoto Hideo , Sumariyono  
Published: February 2022

AbstractAimS‐flurbiprofen plaster (SFPP) is a novel topical nonsteroidal anti‐inflammatory drug (NSAID) patch. This study aimed to assess the efficacy and safety of SFPP in knee osteoarthritis (OA) patients compared to diclofenac gel.MethodsThis study was a multicenter, randomized, active‐controlled, open‐label, non‐inferiority phase III trial. There were 311 enrolled patients treated by SFPP or diclofenac gel for 2 weeks. The primary efficacy outcome was the knee pain when rising from the specially arranged chair assessed by visual analog scale (rVAS). The other efficacy outcomes were clinical symptoms, pain on walking, global assessment by both investigator and patient, and use/non‐use of the rescue drugs during the treatment period. Adverse events (AEs) were evaluated as the safety outcome.ResultsThe least‐squares mean (95% CI) of ΔrVAS at the end of the study was 41.52 (39.16‐43.88) mm in the SFPP group and 36.01 (33.69‐38.33) mm in the diclofenac gel group, with a between‐group difference of 5.51 (2.20‐8.82), indicating non‐inferiority. There were statistically significant differences between the groups in rVAS, clinical symptoms, pain on walking, and the global assessment by both investigator and patient. The incidence rate of AEs in the SFPP group was 5.8%, and there was no statistically significant difference from that in the diclofenac gel group (5.2%). Most of the AEs were mild, and no AE led to discontinuation.ConclusionNon‐inferiority of SFPP to diclofenac gel was demonstrated in the efficacy for pain on rising from a chair. SFPP was also well‐tolerated in knee OA patients.
Sex pheromones and sex attractants of species within the genera Idolus Desbrochers des Loges and Dalopius Eschscholtz (Coleoptera: Elateridae) in the western United States
Authors: Serrano Jacqueline M. , Landolt Peter J. , Reyes Corral Cesar A. , Millar Jocelyn G. 
Published: February 2022

Abstract
Most known click beetle (Coleoptera: Elateridae) sex pheromones have been identified from economically important European species. We hypothesized that pheromone components might be conserved between New and Old World species, and so tested blends of known pheromones in California and Washington states.
Males of the endemic species Idolus californicus and Idolus columbianus (subfamily Elaterinae) were attracted to blends of neryl esters, many of which are known pheromones of related European species. Electroantennogram assays determined that antennae of male beetles responded strongly to two neryl esters, and in field bioassays, males of both species were specifically attracted to neryl hexanoate.
Female I. californicus were found to produce both neryl hexanoate and neryl octanoate, and lures that contained neryl hexanoate alone or in blends attracted male beetles. Neryl octanoate was not attractive, and neither synergized nor antagonized attraction to neryl hexanoate.
Males of Dalopius spp. also were captured in traps baited with lures containing neryl octanoate.
Our results support the hypothesis that the neryl ester pheromone motif is conserved among taxonomically related European and North American elaterids in the tribe Elaterinae, which should aid in the identification of pheromones of pest species within the tribe.
Trans‐homophilic interaction of CADM1 promotes organ infiltration of T‐cell lymphoma by adhesion to vascular endothelium
Authors: Kasai Yutaka , Gan Siew Pey , Funaki Toko , Ohashi‐Kumagai Yuki , Tominaga Mizuki , Shiu Shu‐Jen , Suzuki Daisuke , Matsubara Daisuke , Sakamoto Takeharu , Sakurai‐Yageta Mika , Ito Takeshi , Murakami Yoshinori 
Published: February 2022

AbstractThe initial step of organ infiltration of malignant cells is the interaction with host vascular endothelial cells, which is often mediated by specific combinations of cell adhesion molecules. Cell adhesion molecule 1 (CADM1) is overexpressed in adult T‐cell leukemia/lymphoma (ATL) and provides a cell‐surface diagnostic marker. CADM1 promotes the adhesion of ATL cells to vascular endothelial cells and multiple organ infiltration in mice. However, its binding partner on host cells has not yet been identified. In this study, we show that CADM1 promotes transendothelial migration of ATL cells in addition to the adhesion to vascular endothelial cells. Moreover, CADM1 enhances liver infiltration of mouse T‐cell lymphoma cells, EL4, after tail vein injection, whereas a CADM1 mutant lacking adhesive activity did not. Among the known CADM1‐binding proteins expressed in primary endothelial cells, only CADM1 and CADM4 could induce morphological extension of ATL cells when plated onto glass coated with these proteins. Furthermore, CADM1‐mediated liver infiltration of EL4 cells was canceled in conventional and vascular endothelium‐specific Cadm1 knockout mice, whereas it was not canceled in Cadm4 knockout mice. These results suggest that CADM1 on host vascular endothelial cells is required for organ infiltration of ATL and other T‐cell lymphomas expressing CADM1.
Type I conventional dendritic cells relate to disease severity in virus‐induced asthma exacerbations
Authors: Cameron Aoife , Dhariwal Jaideep , Upton Nadine , Ranz Jimenez Ismael , Paulsen Malte , Wong Ernie , Trujillo‐Torralbo Maria‐Belen , del Rosario Ajerico , Jackson David J. , Edwards Michael R. , Johnston Sebastian L. , Walton Ross P. ,   the MRC‐GSK Strategic Alliance Consortium
Published: February 2022

AbstractRationaleRhinoviruses are the major precipitant of asthma exacerbations and individuals with asthma experience more severe/prolonged rhinovirus infections. Concurrent viral infection and allergen exposure synergistically increase exacerbation risk. Although dendritic cells orchestrate immune responses to both virus and allergen, little is known about their role in viral asthma exacerbations.ObjectivesTo characterize dendritic cell populations present in the lower airways, and to assess whether their numbers are altered in asthma compared to healthy subjects prior to infection and during rhinovirus‐16 infection.MethodsModerately‐severe atopic asthmatic patients and healthy controls were experimentally infected with rhinovirus‐16. Bronchoalveolar lavage was collected at baseline, day 3 and day 8 post infection and dendritic cells isolated using fluorescence activated cell sorting.Measurements and Main ResultsNumbers of type I conventional dendritic cells, which cross prime CD8+ T helper cells and produce innate interferons, were significantly reduced in the lower airways of asthma patients compared to healthy controls at baseline. This reduction was associated serum IgE at baseline and with reduced numbers of CD8+ T helper cells and with increased viral replication, airway eosinophils and reduced lung function during infection. IgE receptor expression on lower airway plasmacytoid dendritic cells was significantly increased in asthma, consistent with a reduced capacity to produce innate interferons.ConclusionsReduced numbers of anti‐viral type I conventional dendritic cells in asthma are associated with adverse outcomes during rhinovirus infection. This, with increased FcεR1α expression on lower airway plasmacytoid DCs could mediate the more permissive respiratory viral infection observed in asthma patients.
Severe COVID‐19 pneumonia in an intensive care setting and comparisons with historic severe viral pneumonia due to other viruses
Authors: Dadhwal Kiran , Stonham Rosalind , Breen Hannah , Poole Stephen , Saeed Kordo , Dushianthan Ahilanandan 
Published: February 2022

AbstractPurposeSevere viral pneumonia is associated with significant morbidity and mortality. Recent COVID‐19 pandemic continues to impose significant health burden worldwide, and individual pandemic waves often lead to a large surge in the intensive care unit (ICU) admissions for respiratory support. Comparisons of severe SARS‐CoV‐2 pneumonia with other seasonal and nonseasonal severe viral infections are rarely studied in an intensive care setting.MethodsA retrospective cohort study comparing patients admitted to ICU with COVID‐19 between March and June 2020 and those with viral pneumonias between January and December 2019. We compared patient specific demographic variables, duration of illness, ICU organ supportive measures and outcomes between both groups.ResultsAnalysis of 93 COVID‐19 (Group 1) and 52 other viral pneumonia patients (Group 2) showed an increased proportion of obesity (42% vs. 23%, p = 0.02), non‐White ethnicities (41% vs. 6%, p < 0.001) and diabetes mellitus (30% vs. 13%, p = 0.03) in Group 1, with lower prevalence of chronic obstructive pulmonary disease (COPD)/asthma (16% vs. 34%, p = 0.02). In Group 1, the neutrophil to lymphocyte ratio was much lower (6.7 vs. 10, p = 0.006), and invasive mechanical ventilation (58% vs. 26%, p < 0.001) was more common. Length of ICU (8 vs. 4, p < 0.001) and hospital stay (22 vs. 11, p < 0.001) was prolonged in Group 1, with no significant difference in mortality. Influenza A and rhinovirus were the most common pathogens in Group 2 (26% each).ConclusionsKey differences were identified within demographics (obesity, ethnicity, age, ICU scores, comorbidities) and organ support. Despite these variations, there were no significant differences in mortality between both groups. Further studies with larger sample sizes would allow for further assessment of clinical parameters in these patients.
Prognostic significance of blood urea nitrogen/creatinine ratio in chronic HFpEF
Authors: Zhen Zhe , Liang Weihao , Tan Weiping , Dong Bin , Wu Yuzhong , Liu Chen , Xue Ruicong 
Published: February 2022

AbstractBackgroundThe prognostic significance of blood urea nitrogen (BUN)/creatinine ratio specifically in chronic heart failure with preserved ejection fraction (HFpEF) patients remained unclear. We aimed to evaluate the association of BUN/creatinine ratio (baseline level and visit‐to‐visit variation) with the risk of adverse clinical outcomes among patients with chronic HFpEF.Methods and resultsThis is a secondary analysis of the Treatment of Preserved Cardiac Function Heart Failure with an Aldosterone Antagonist (TOPCAT) trial. Of the enrolled 3445 participants in the TOPCAT trial, associations between BUN/creatinine and clinical outcomes were examined in a subset of 1521 (baseline measurements level) and 1453 (visit‐to‐visit variation) participants. A multivariable Cox proportional hazard model was used to assess the prognostic significance of BUN/creatinine ratio and BUN/creatinine ratio variation for the prespecified clinical outcomes. A higher BUN/creatinine ratio was associated with a higher risk of all‐cause mortality (hazard ratio [HR] = 1.52, 95%CI, 1.21‐1.91; p < .001) as well as cardiovascular disease mortality (HR = 1.83, 95%CI, 1.35‐2.49; p < .001) in the fully adjusted model. Greater visit‐to‐visit variability in BUN/creatinine ratio tended to be independently associated with a higher risk of heart failure hospitalization and primary endpoint (p < .001 for both outcomes). Furthermore, those findings were consistent across participants stratified by the presence of chronic kidney disease at baseline.ConclusionsHigher BUN/creatinine ratio and greater BUN/creatinine ratio variability are independently associated with adverse outcomes in HFpEF participants in the TOPCAT trial.