bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combining data acquisition modes in liquid-chromatography–tandem mass spectrometry for comprehensive determination of acylcarnitines in human serum
Authors: Luque-Córdoba D. , Calderón-Santiago M. , Priego-Capote F. 
Published: July 2022

AbstractAcylcarnitines (ACs) are metabolites involved in fatty acid β-oxidation and organic acid metabolism. Metabolic disorders associated to these two processes can be evaluated by determining the complete profile of ACs. In this research, we present an overall strategy for identification, confirmation, and quantitative determination of acylcarnitines in human serum. By this strategy we identified the presence of 47 ACs from C2 to C24 with detection of the unsaturation degree by application of a data-independent acquisition (DIA) liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. Complementary, quantitative determination of ACs is based on a high-throughput and fully automated method consisting of solid-phase extraction on-line coupled to LC–MS/MS in data-dependent acquisition (DDA) to improve analytical features avoiding the errors associated to sample processing. Quantitation limits were at pg mL–1 level, the intra-day and between-day variability were below 15–20%, respectively; and the accuracy, expressed as bias, was always within ± 25%. The proposed method was tested with 40 human volunteers to determine the relative concentration of ACs in serum and identify predominant forms. Significant differences were detected by comparing the ACs profile of obese versus non-obese individuals.
Metabolic changes associated with two endocrine abnormalities in dogs: elevated fructosamine and low thyroxine
Authors: Ottka Claudia , Puurunen Jenni , Müller Elisabeth , Weber Corinna , Klein Ruth , Lohi Hannes 
Published: July 2022

Abstract
Introduction
Metabolomics studies in canine endocrine abnormalities are sparse and basic information on these abnormalities must be generated.

Objectives
To characterize the metabolic changes associated with elevated fructosamine, reflecting poor glycemic control, and low thyroxine, a thyroid hormone controlling metabolism.

Methods
Leftovers of clinical serum samples; 25 controls, 79 high fructosamine, and 47 low thyroxine, were analyzed using 1H NMR and differences were evaluated using Firth logistic regression.

Results
Both high fructosamine and low thyroxine were associated with changes in concentrations of multiple metabolites, including glycoprotein acetyls and lipids.

Conclusion
These findings suggest promising makers for further research and clinical validation.
Carbon dots from eco-friendly precursors for optical sensing application: an up-to-date review
Authors: Ullal Namratha , Muthamma Kashmitha , Sunil Dhanya 
Published: July 2022

Abstract
Carbon dots (CDs) are zero-dimensional quasi-spherical nanoparticles endowed with excellent advantages including good luminescence features, photostability, low cytotoxicity, remarkable aqueous solubility, favourable biocompatibility, low risk to environment and great flexibility in surface modification. Fluorescent CDs that can selectively respond to specific inorganic/organic target molecules in environmental and biological samples are of prime significance amongst the new generation intelligent sensors due to the critical involvement of different ions/molecular species in not only human health, but also in environment processes. In this context, preparation of CDs from bioprecursors has immense significance due to the involvement of green principles, inexpensive, clean, nontoxic, easily accessible, renewable and large-scale production can be realized. This article aims at exploring different types of green raw materials including plant biomass, animal products, food items and waste materials as carbon sources for the synthesis of both undoped and doped CDs. The emphasis is given on different synthetic approaches adopted for improving the quantum yield without any chemical modification, the characterization techniques, mechanistic origin of photoluminescence and fluorescence response mechanisms involved in the sensing action towards various analytes. The significant benefits and limitations of CDs obtained from eco-friendly precursors through green approaches are summarized. Various challenges and the future prospects of these carbonaceous nanomaterials as sensors are also discussed.

Graphical abstract
Peritoneal Metastases from Extraperitoneal Primary Tumors: Incidence, Treatment, and Survival from a Nationwide Database
Authors: Rijken Anouk , Galanos Laskarina J. K. , Burger Jacobus W. A. , Nienhuijs Simon W. , van Erning Felice N. , de Hingh Ignace H. J. T. 
Published: July 2022

Abstract
The objective of this study was to assess the incidence, treatment, and survival of patients with synchronous peritoneal metastases (PM) from extraperitoneal primary tumors. A cohort was selected from the Netherlands Cancer Registry (NCR), in which all patients diagnosed with PM in 2017 and 2018 were screened for eligibility. The five most common primary extraperitoneal origins of PM were included for further analyses: lung, breast, urinary tract, and kidney cancer and malignant melanoma. Survival was investigated using log-rank test between different primary tumor locations. In total, 480 patients were diagnosed with synchronous PM from extraperitoneal origins. The proportion of patients with PM per extraperitoneal origin ranged between 0.1 and 1.1%, with the highest proportion in lung cancer patients. Of all patients, 234 (49%) received tumor-directed treatment and 246 (51%) received no tumor-directed treatment. Survival in patients with PM from lung, breast, urinary tract, and kidney cancer and malignant melanoma was 1.6 months, 15.7 months, 5.4 months, 3.4 months, and 2.1 months, respectively (p < 0.001). In this study, a small, although significant number of patients with extraperitoneal cancer developed PM. The reported survival in patients with PM ranged between 1.6 and 15.7 months. Only half of the patients with PM received tumor-directed treatment and survival in patients without tumor-directed treatment was only 1.2 months. These findings are stressing the need to explore new diagnostic tools that may enable earlier diagnosis of PM and may potentially lead to a more effective treatment.
Quantitative weighted estimates for Harmonic analysis operators in the Bessel setting by using sparse domination
Authors: Almeida Víctor , Betancor Jorge J. , Fariña Juan C. , Rodríguez-Mesa Lourdes 
Published: July 2022

AbstractIn this paper we obtain quantitative weighted $$L^p$$

L
p

-inequalities for some operators involving Bessel convolutions. We consider maximal operators, Littlewood-Paley functions and variational operators. We obtain $$L^p(w)$$

L
p

(
w
)

-operator norms in terms of the $$A_p$$

A
p

-characteristic of the weight w. In order to do this we show that the operators under consideration are dominated by a suitable family of sparse operators in the space of homogeneous type $$((0,\infty ),|\cdot |,x^{2\lambda }dx)$$

(

(
0
,
∞
)

,
|
·
|
,

x

2
λ

d
x
)

.
AFFINE LOGIC FOR CONSTRUCTIVE MATHEMATICS
Authors: SHULMAN MICHAEL 
Published: July 2022

AbstractWe show that numerous distinctive concepts of constructive mathematics arise automatically from an “antithesis” translation of affine logic into intuitionistic logic via a Chu/Dialectica construction. This includes apartness relations, complemented subsets, anti-subgroups and anti-ideals, strict and non-strict order pairs, cut-valued metrics, and apartness spaces. We also explain the constructive bifurcation of some classical concepts using the choice between multiplicative and additive affine connectives. Affine logic and the antithesis construction thus systematically “constructivize” classical definitions, handling the resulting bookkeeping automatically.
Zinc, sulfur and cadmium isotopes and Zn/Cd ratios as indicators of the origin of the supergiant Broken Hill Pb–Zn–Ag deposit and other Broken Hill-type deposits, New South Wales, Australia
Authors: Spry Paul G. , Mathur Ryan D. , Teale Graham S. , Godfrey Linda V. 
Published: July 2022

AbstractVarious genetic models have been proposed for the supergiant Proterozoic Broken Hill Pb–Zn–Ag deposit largely based on geological and geochronological evidence. Here we present Zn, Cd and S isotope compositions as well as Zn/Cd ratios of sphalerite from Broken Hill and minor Broken Hill-type deposits (Australia) to help constrain these models but focus on syngenetic and magmatic–hydrothermal processes, since epigenetic models can be rejected because the orebodies were deformed and metamorphosed by the Olarian Orogeny. Values of δ34SVCDT, δ66ZnAA-ETHand δ114CdNIST SRM 3108for sphalerite from Broken Hill range from +0.27 to +4.73 ‰, −1.15 to +0.46 ‰ and −0.48 to +0.01 ‰, respectively, while those for the smaller Broken Hill-type deposits range from −5.11 to +1.28 ‰, −0.97 to +0.10 ‰ and −1.02 to +2.59 ‰, respectively. By combining published S isotope data of sulfides from the Broken Hill district with those obtained here, the sources of sulfur via thermochemical sulfate reduction, bacterial sulfate reduction and a magmatic origin cannot be distinguished. However, when the S isotope compositions are considered along with the broad range of Cd and Zn isotope data for sphalerite, which are among the lightest and heaviest yet reported for a sulfide deposit, the isotopic datasets are consistent with low-temperature biogenic processes associated with syngenetic deposition of sulfides. Cadmium isotope compositions when coupled with Zn/Cd ratios of sphalerite have previously been used to classify Pb–Zn deposits, including low-temperature, high-temperature and exhalative ores. However, the Zn/Cd ratios of sphalerite from Broken Hill cannot be used for such classification purposes.
Influence of climate stress on technical efficiency and economic downside risk exposure of EU dairy farms
Authors: Quiédeville Sylvain , Grovermann Christian , Leiber Florian , Cozzi Giulio , Lora Isabella , Eory Vera , Moakes Simon 
Published: July 2022

AbstractThis paper assesses the influence of heat and drought stress on the economic performance of the European dairy sector. Climatic data from the Gridded Agro-Meteorological data in Europe were combined with dairy enterprise data from the Farm Accountancy Data Network, resulting in a data set of 4412 farms in 22 European Union (EU) countries over the period 2007–2013. Since the performance of dairy farms is influenced by the context in which they operate, farms were grouped into areas representing similar climatic conditions through the use of a latent class analysis. Technical efficiency (TE) and economic downside risk were used as performance indicators against which the effect of climatic stress factors was evaluated. TE was estimated using a ‘true-fixed’ effect stochastic frontier model. Economic downside risk was based on gross margin deviations. Regression analysis suggests a significant negative effect of drought and heat stress on both TE and the downside gross margin difference in most climatic classes, with few exceptions. Results imply that both drought and heat stress-related issues need to be considered when designing adaptation strategies to address threats to the economic performance of the EU dairy sector.
Effects of environmental factors on ichthyoplankton in a permanently open estuary under the influence of a semi-arid climate, north-eastern Brazil
Authors: Gomes de Lima Lidiane , Juan Soterorudá Brito Gitá , Pessanha André Luiz Machado 
Published: July 2022

AbstractEstuarine ecosystem conditions actively influence the early life stage of fishes. This study reports how environmental factors influenced the ichthyoplankton in a tropical estuary within an Environmental Protection Area by comparing the structure and composition of fish eggs and larval assemblages. A total of 1672 fish larvae and 486 fish eggs were collected. Higher densities of larvae were recorded for Engraulidae, Characidae, Clupeidae, Gerreidae, Mugilidae and Atherinopsidae, and higher egg densities of the families Mugilidae, Clupeidae and Engraulidae were found. The spatio-temporal variations were determined by the environmental predictors salinity, pH, dissolved oxygen and temperature, with salinity influenced by precipitation as one of the main predictors of the distribution of ichthyoplankton. During the rainy season, greater densities of eggs were recorded in the upper and intermediate zones, mainly Characidae and Engraulidae; in the dry season, in the lower zone, there was a greater density of larvae, particularly Atherinopsidae and Mugilidae. The information provided in the present study contributes to our knowledge of nursery habitat requirements for the initial development of marine migrant and resident species in tropical estuaries.
A systematic review of clinical study evidence for pulmonary vasodilator therapy following surgery with cardiopulmonary bypass in children with CHD
Authors: Foote Henry P. , Hornik Christoph P. , Hill Kevin D. , Rotta Alexandre T. , Kumar Karan R. , Thompson Elizabeth J. 
Published: July 2022

AbstractObjectives:Complications from pulmonary hypertension are one of the leading contributors to morbidity and mortality post-cardiopulmonary bypass surgery in children with CHD. Pulmonary vasodilator therapies are commonly used post-operatively, but the optimal target patient population, therapy choice, timing of therapy initiation, and duration of therapy are not well defined.Methods:We used PubMed and EMBASE to identify studies from 2000 to 2020 investigating the use of pulmonary vasodilator therapy post-cardiopulmonary bypass in children aged 0–18 years. To ensure eligibility criteria, studies were systematically reviewed by two independent reviewers.Results:We identified 26 studies of 42,971 children across four medication classes; 23 were single centre, 14 were prospective, and 11 involved randomisation (four of which employed a placebo-control arm). A disproportionate number of children were from a single retrospective study of 41,872 patients. Definitions varied, but change in pulmonary haemodynamics was the most common primary outcome, used in 14 studies. Six studies had clinical endpoints, with mortality the primary endpoint for two studies. Treatment with inhaled nitric oxide, iloprost, and sildenafil all resulted in improved haemodynamics in specific cohorts of children with post-operative pulmonary hypertension, although improved outcomes were not consistently demonstrated across all treated children. Iloprost may be a cheaper alternative to inhaled nitric oxide with similar haemodynamic response.Conclusion:Studies were predominantly single-centre, a control arm was rarely used in randomised studies, and haemodynamic endpoints varied significantly. Further research is needed to reduce post-operative morbidity and mortality from pulmonary hypertension in children with CHD.
Persistence of Anxiety/Depression Symptoms in Early Adolescence: A Prospective Study of Daily Life Stress, Rumination, and Daytime Sleepiness in a Genetically Informative Cohort
Authors: Hansell Narelle K. , Strike Lachlan T. , de Zubicaray Greig I. , Thompson Paul M. , McMahon Katie L. , Wright Margaret J. 
Published: July 2022

AbstractIn this prospective study of mental health, we examine the influence of three interrelated traits — perceived stress, rumination, and daytime sleepiness — and their association with symptoms of anxiety and depression in early adolescence. Given the known associations between these traits, an important objective is to determine the extent to which they may independently predict anxiety/depression symptoms. Twin pairs from the Queensland Twin Adolescent Brain (QTAB) project were assessed on two occasions (N= 211 pairs aged 9−14 years at baseline and 152 pairs aged 10−16 years at follow-up). Linear regression models and quantitative genetic modeling were used to analyze the data. Prospectively, perceived stress, rumination, and daytime sleepiness accounted for 8−11% of the variation in later anxiety/depression; familial influences contributed strongly to these associations. However, only perceived stress significantly predicted change in anxiety/depression, accounting for 3% of variance at follow-up after adjusting for anxiety/depression at baseline, although it did not do so independently of rumination and daytime sleepiness. Bidirectional effects were found between all traits over time. These findings suggest an underlying architecture that is shared, to some degree, by all traits, while the literature points to hypothalamic–pituitary–adrenal (HPA) axis and/or circadian systems as potential sources of overlapping influence and possible avenues for intervention.
Isotopic Equilibration in Convective Downdrafts
Authors: Torri Giuseppe 
Published: July 2022

AbstractWhile it is becoming increasingly clear that stable water isotopologues are important tools in the study of atmospheric convection, many questions remain on how different processes affect them. This work is focused on water vapor isotopes in precipitation‐driven downdrafts. Using idealized simulations, the contributions of rain evaporation and liquid‐vapor equilibration processes are analyzed. It is shown that rain evaporation tends to deplete water vapor isotopes throughout the downdraft's descent, whereas equilibration has a neutral or depleting effect in the first half of the downdraft's life cycle and an enriching one in the second half. The total contribution of the two processes is then discussed, and it is argued that equilibration has an overall small effect compared to rain evaporation. Finally, using two sensitivity experiments, it is shown that the role played by equilibration varies significantly in regimes with different concentrations of cloud condensation nuclei.
Tropospheric Response to Stratospheric Variability via Lagged Quantile Regression
Authors: Finke K. , Hannachi A. 
Published: July 2022

AbstractStratospheric variability may affect tropospheric weather on time scales between a few weeks and a few months, thus providing great potential for sub‐seasonal to seasonal weather forecasts of extremes. Unlike standard linear regression, which is based on the conditional mean of the response variable, lagged quantile regression is used here, which enables modeling the response variable's complete conditional distribution. Using the ERA5 reanalysis, we explore the relation of prominent teleconnection indices and gridded tropospheric fields to the stratosphere during boreal winter for various quantiles and lags. Significant differences in duration, strength, and direction of the stratosphere‐troposphere connection in outer quantiles compared to the median are identified. In particular, tropospheric extremes and their relation to the polar vortex strength are discussed, and an outlook is given.
Activity-based protein profiling identifies alternating activation of enzymes involved in the bifidobacterium shunt pathway or mucin degradation in the gut microbiome response to soluble dietary fiber
Authors: Killinger Bryan J. , Whidbey Christopher , Sadler Natalie C. , DeLeon Adrian J. , Munoz Nathalie , Kim Young-Mo , Wright Aaron T. 
Published: July 2022

AbstractWhile deprivation of dietary fiber has been associated with adverse health outcomes, investigations concerning the effect of dietary fiber on the gut microbiome have been largely limited to compositional sequence-based analyses or utilize a defined microbiota not native to the host. To extend understanding of the microbiome’s functional response to dietary fiber deprivation beyond correlative evidence from sequence-based analyses, approaches capable of measuring functional enzymatic activity are needed. In this study, we use an activity-based protein profiling (ABPP) approach to identify sugar metabolizing and transport proteins in native mouse gut microbiomes that respond with differential activity to the deprivation or supplementation of the soluble dietary fibers inulin and pectin. We found that the microbiome of mice subjected to a high fiber diet high in soluble fiber had increased functional activity of multiple proteins, including glycoside hydrolases, polysaccharide lyases, and sugar transport proteins from diverse taxa. The results point to an increase in activity of theBifidobacteriumshunt metabolic pathway in the microbiome of mice fed high fiber diets. In those subjected to a low fiber diet, we identified a shift from the degradation of dietary fibers to that of gut mucins, in particular by the recently isolated taxon “Musculibacterium intestinale”, which experienced dramatic growth in response to fiber deprivation. When combined with metabolomics and shotgun metagenomics analyses, our findings provide a functional investigation of dietary fiber metabolism in the gut microbiome and demonstrates the power of a combined ABPP-multiomics approach for characterizing the response of the gut microbiome to perturbations.
Differential survival of potentially pathogenic, septicemia- and meningitis-causing E. coli across the wastewater treatment train
Authors: Yu Daniel , Ryu Kanghee , Otto Simon J. G. , Stothard Paul , Banting Graham , Ruecker Norma , Neumann Norman F. , Zhi Shuai 
Published: July 2022

AbstractA growing body of evidence indicates that extraintestinal pathogenic E. coli (ExPEC) readily survive wastewater treatment, raising concerns about the public health risks associated with exposure to wastewater-contaminated environments. In this study, E. coli isolates recovered from chlorinated sewage or treated wastewater effluents in Canada were screened for ExPEC virulence markers. Eighty-six isolates were identified as presumptive ExPEC, clustering within major pandemic lineages including ST131, ST95, and ST73 according to multilocus sequence typing analyses. Across the whole, core, and accessory genome, 37 isolates were extremely similar to clinical bloodborne E. coli (BBEC) and neonatal meningitic E. coli (NMEC) strains, suggesting that these wastewater isolates may exhibit a similar phenotypically related pathogenic potential. Interestingly, ExPEC strains also shared accessory gene content with naturalized wastewater strains, suggesting a common genetic capacity for surviving water treatment. Collectively, these findings suggest that E. coli strains that may cause septicemia and meningitis are surviving wastewater treatment and may be transmissible through wastewater effluents.
A new elpistostegalian from the Late Devonian of the Canadian Arctic
Authors: Stewart Thomas A. , Lemberg Justin B. , Daly Ailis , Daeschler Edward B. , Shubin Neil H. 
Published: July 2022

AbstractA fundamental gap in the study of the origin of limbed vertebrates lies in understanding the morphological and functional diversity of their closest relatives. Whereas analyses of the elpistostegalians Panderichthys rhombolepis, Tiktaalik roseae and Elpistostege watsoni have revealed a sequence of changes in locomotor, feeding and respiratory structures during the transition1–9, an isolated bone, a putative humerus, has controversially hinted at a wider range in form and function than now recognized10–14. Here we report the discovery of a new elpistostegalian from the Late Devonian period of the Canadian Arctic that shows surprising disparity in the group. The specimen includes partial upper and lower jaws, pharyngeal elements, a pectoral fin and scalation. This new genus is phylogenetically proximate to T. roseae and E. watsoni but evinces notable differences from both taxa and, indeed, other described tetrapodomorphs. Lacking processes, joint orientations and muscle scars indicative of appendage-based support on a hard substrate13, its pectoral fin shows specializations for swimming that are unlike those known from other sarcopterygians. This unexpected morphological and functional diversity represents a previously hidden ecological expansion, a secondary return to open water, near the origin of limbed vertebrates.