bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sistem Kandang Ayam Petelur Otomatis Dengan Memanfaatkan Open-Source Hardware
Authors: Restu Krsna Elian Panji , Liandana Made , Aryanto I Komang Agus Ady , Nirmala Bagus Made Sabda 
Published: August 2022

Peternakan ayam petelur pada umumnya masih melakukan pemberian pakan menggunakan sistem konvensional dengan cara menaburkan pakan pada tempat makan yang berada disepanjang kandang dan terus berpindah dari satu kandang ke kandang lainnya. Kotoran ayam petelur yang masih dibersihkan dengan cara mengeruk kotoran lalu dikumpulkan pada karung atau dengan cara menyemprotkan air, serta pengumpulan telur secara manual dengan cara memungut telur sepanjang kandang. Kegiatan tersebut masih kurang efektif dalam melakukan perawatan dan pemeliharaan kandang dikarenakan masih membutuhkan banyak pekerjaan. Oleh karena itu diperlukan fasilitas kandang ayam petelur yang dapat bekerja secara otomatis. Kandang ayam petelur secara otomatis ini dapat membantu para peternak ayam petelur dalam memberikan pakan, membersihkan kotoran, mengumpulkan telur ayam secara otomatis dan menghitung hasil telur secara mudah serta dapat memonitoring suhu dan kelembapan kandang ayam petelur secara real time. sistem ini menggunakan modul-modul dan sensor-sensor yang terkait dan terhubung dengan mikrokontroler yang dapat dimonitor oleh pengguna melalui aplikasi Blynk pada smartphone pengguna. Sistem yang dibangun menggunakan komponen-komponen yang sudah ada berupa modul – modul sensor yang mudah untuk didapatkan dipasaran dengan harga yang terjangkau yaitu penggunaan Arduino Atmega2560 sebagai mikrokontroler, LCD untuk menampilkan hasil data yang di dapat pada kandang, Relay untuk switch motor kotoran dan pengumpulan telur, motor driver Ln298 sebagai pengatur pergerakan motor, Esp8288 untuk jaringan wifi, DHT22 untuk pengukuran suhu dan kelembapan pada kandang, serta RTC DS3231 untuk pewaktuan digital pada kandang. Alat alat tersebut dibangun tidak terlalu kompleks agar peternak atau pengguna dapat mudah dalam menggunakan dan melakukan perawatan apabila salah satu komponen dari alat mengalami kerusakan sehingga mudah untuk diganti.
OS EFEITOS DA PANDEMIA DO NOVO CORONAVÍRUS: UM ESTUDO DAS ESTRATÉGIAS DE MINTZBERG, AHLSTRAND E LAMPEL (2010) ADOTADAS POR DOIS EMPREENDEDORES GAÚCHOS
Authors: Gonzato Rodrigues dos Santos Nicole Carolina , Rosa Ana Claudia da , Melz Rodrigo Luis 
Published: August 2022

A atual pesquisa buscou identificar as estratégias utilizadas por empreendedores de uma cidade do Rio Grande do Sul, especificamente a cidade de Frederico Westphalen/RS, perante a crise provocada pela Covid-19. Para isso, realizou-se um estudo qualitativo, de natureza aplicada e exploratória, a partir de uma pesquisa descritiva, com a coleta de dados por meio de um estudo de caso, a partir de entrevistas semiestruturadas diretamente com dois empreendedores locais, sendo um do segmento de bares e outro do segmento de academia. Os resultados trouxeram como contribuições a descrição e compreensão das estratégias adotadas pelas empresas em foco.
Palavras-Chave: Planejamento. Estratégia. Pandemia. Empreendedores.

Abstract: The current research sought to identify the strategies used by entrepreneurs in a city in Rio Grande do Sul, specifically the city of Frederico Westphalen/RS, in the face of the crisis caused by Covid-19. For this, a qualitative, applied and exploratory study was carried out, based on a descriptive research, with data collection through a case study, from semi-structured interviews directly with two local entrepreneurs, one of the bar segment and another in the gym segment. The results brought contributions the description and understanding of the strategies adopted by the companies in focus.
Keywords: Planning. Strategy. Pandemic. Entrepreneurs.
Percepção do ensino remoto em crianças e adolescentes com diagnóstico de TDAH, seus pais e professores
Authors: Tanaka Amanda de Oliveira , Bissi Stéfani da Silveira , Rossetto Alessandra de Arriba 
Published: August 2022

O SARS-CoV-2, conhecido popularmente como Coronavírus, foi identificado em Wuhan, na China, em dezembro de 2019. A disseminação contagiosa acelerada da COVID-19 levou a Organização Mundial da Saúde a decretar Emergência de Saúde Pública de Importância Internacional (ESPII) em 2020. Para conter sua rápida disseminação, medidas de distanciamento social foram adotadas acarretando desafios nos diferentes contextos. No âmbito educacional, o Ministério da Educação decretou a substituição das aulas presenciais pelo ensino remoto emergencial. O TDAH é um transtorno neurobiológico caracterizado por um padrão persistente de desatenção e/ou hiperatividade-impulsividade, que interfere no funcionamento, no desenvolvimento e no processo de ensino-aprendizagem dos portadores desta condição. Estudos anteriores apontaram as implicações que as novas tecnologias digitais podem ter no processo de aprendizagem/desenvolvimento de escolares com TDAH. Igualmente, foi destacado que a metodologia didática usada pelos professores deve favorecer o desenvolvimento e comportamento da criança com TDAH. Neste contexto, foi realizada uma revisão bibliográfica para descrever os resultados das pesquisas que avaliam a percepção do ensino remoto em crianças com diagnóstico de TDAH, seus pais e professores, para conhecer as facilidades e as dificuldades no uso dos ambientes virtuais de aprendizagem. Observam-se resultados inconsistentes sobre o impacto do ensino remoto nas crianças e adolescentes com TDAH, pois alguns estudos apontam benefícios e outros malefícios. Conclui-se a necessidade de futuras pesquisas para esclarecer as disparidades e os fatores que interferem nos resultados e facilitem ou dificultem o processo de ensino-aprendizagem em alunos com TDAH.
Construção de uma prática terapêutica ocupacional com crianças com atraso no desenvolvimento: Relato de Experiência
Authors: Silva Laryssa Celly Rodrigues da , Paiva Rayssa Béder César , Albuquerque Raquel Costa 
Published: August 2022

Objetivo: Este estudo objetivou compreender de que forma estrutura-se a prática terapêutica ocupacional cujo objetivo é favorecer o desempenho ocupacional de crianças com AD em suas ocupações. Metodologia: Trata-se de um relato de experiência construído a partir das vivências na disciplina Estágio Supervisionado em Terapia Ocupacional 1, ministrada no sétimo período do curso de Terapia Ocupacional da Universidade Federal de Pernambuco (UFPE), em uma instituição sem fins lucrativos localizada no bairro do Recife. Foram realizados atendimentos semanais com crianças com diferentes diagnósticos, como Síndrome de Down e Transtorno do Espectro Autista. Resultados e Discussão: As intervenções foram estruturadas de forma gradual, iniciando a partir da aplicação da anamnese e avaliações com a família e a criança. O brincar foi utilizado como instrumento para favorecer a criação do vínculo e mediar o desenvolvimento de habilidades necessárias para o desempenho ocupacional. Verificou-se a partir da análise de evoluções o aprimoramento de habilidades antes não exploradas pelo indivíduo que favoreceram a execução das ocupações. Conclusão: Devido ao curto período para reaplicação dos instrumentos não se obteve o resultado qualitativo dessa evolução, sendo registrada apenas através da observação. No entanto, tornou-se perceptível o potencial da intervenção terapêutica ocupacional através de uma prática realizada de forma gradual e estruturada, realizando junto a um profissional formado desde a aplicação de instrumentos, desenvolvimento de habilidades isoladas, até o treino da atividade.
Impacto da pandemia do SARS-CoV-2 no rastreamento do câncer de colo de útero no município de Taboão da Serra - SP
Authors: Vieira Camila Maria , Cepeda Aline do Nascimento , Souza Larissa Nínive Santos de , Cardozo Mariah Marrara , Carbone Luís Felipe Barreiras , Sgroi Jaqueline Christiane Lanaro 
Published: August 2022

No Brasil a Atenção Primária à Saúde é responsável pelo rastreamento precoce de neoplasias. O Câncer de Colo de Útero está em segundo lugar como o câncer mais prevalente no país. Seu método de rastreamento é o exame de colpocitologia oncótica realizado em mulheres entre 25 a 64 anos com vida sexual ativa. Objetivo: O presente estudo tem como objetivo analisar o impacto da pandemia do vírus SARS-CoV-2 no rastreamento do câncer de colo de útero no município de Taboão da Serra - SP. Método: foi um estudo observacional transversal, com análise de bases de dados fornecidos pelo município de Taboão da Serra. Resultado: constatou-se que, no respectivo município, o total de exames colpocitológicos nas mulheres de idade elegível (25 - 64 anos), coletados em 2019 foi de 6.276 e, no ano seguinte, 2020, foi de 4.207. Houve uma redução absoluta de 2.069 e relativa de 32,97%. Conclusão: o estudo permitiu analisar o impacto da pandemia de SARS-CoV-2 no rastreamento do Câncer de Colo de Útero, no entanto também foi observado que no ano que antecede a pandemia o rastreamento no município também era deficitário, assim não há uma garantia que esse rastreamento esteja sendo eficaz para reduzir a morbimortalidade.
The effect of mandibular flexure on the design of implant-supported fixed restorations of different facial types under two loading conditions by three-dimensional finite element analysis
Authors: Gao Jing , Li Xuejing , He Jing , Jiang Lulu , Zhao Baohong 
Published: August 2022

Objective: Investigating the biomechanical effects of mandibular flexure (MF) on the design of implant-supported fixed restorations in edentulous jaws of different facial types.Methods: Three-dimensional finite element models were established to analyze mandibular displacement and stress distribution of implant-supported fixed restorations (four or six implants, different implant numbers and sites, and the design of the superstructure across the dental arch in one or two or three pieces, under the loading conditions of maximum opening or right unilateral molar occlusion) in mandibular edentulous patients of three different facial types (brachyfacial, mesofacial, and dolichofacial types).Results: The brachyfacial type presented higher mandibular flexure and stress in the overall restorative system, followed by the mesofacial and dolichofacial types. During jaw opening and occlusal movements, the one-piece framework showed the lowest bone stress values surrounding the anterior implants and gradually increased to the distal position, and the three-piece framework showed the highest stress values for peri-implant bones. Also, the split framework could greatly increase the stress on abutments and frameworks. Moreover, fixed implant prostheses with cantilevers can generate high amounts of biomechanical stress and strain on implants and surrounding bones. The bone surrounding the anterior implant increased in stress values as the most distal implants were more distally located regardless of frameworks. The zirconia framework demonstrated higher stresses than the titanium framework.Conclusion: The design of edentulous fixed implant-supported restorations can be optimized for facial types. For patients of the brachyfacial type or with high masticatory muscle strength, the non-segmented framework without a cantilever provides an optimal biomechanical environment.
Random genome reduction coupled with polyhydroxybutyrate biosynthesis to facilitate its accumulation in Escherichia coli
Authors: Ma Shuai , Su Tianyuan , Liu Jinming , Wang Qian , Liang Quanfeng , Lu Xuemei , Qi Qingsheng 
Published: August 2022

Genome reduction has been emerged as a powerful tool to construct ideal chassis for synthetic biology. Random genome reduction couple genomic deletion with growth and has the potential to construct optimum genome for a given environment. Recently, we developed a transposon-mediated random deletion (TMRD) method that allows the random and continuous reduction of Escherichia coli genome. Here, to prove its ability in constructing optimal cell factories, we coupled polyhydroxybutyrate (PHB) accumulation with random genome reduction and proceeded to reduce the E. coli genome. Five mutants showed high biomass and PHB yields were selected from 18 candidates after ten rounds of genome reduction. And eight or nine genomic fragments (totally 230.1–270.0 Kb) were deleted in their genomes, encompassing 4.95%–5.82% of the parental MG1655 genome. Most mutants displayed better growth, glucose utilization, protein expression, and significant increase of electroporation efficiency compared with MG1655. The PHB content and concentration enhanced up to 13.3%–37.2% and 60.2%–102.9% when batch fermentation was performed in M9-glucose medium using the five mutants. Particularly, in mutant H16, lacking 5.28% of its genome, the increase of biomass and PHB concentration were more than 50% and 100% compared with MG1655, respectively. This work expands the strategy for creating streamlined chassis to improve the production of high value-added products.
Transcriptional expression of m6A and m5C RNA methyltransferase genes in the brain and fat body of honey bee adult workers
Authors: Bataglia Luana , Simões Zilá Luz Paulino , Nunes Francis Morais Franco 
Published: August 2022

Honey bee (Apis mellifera) adult workers change behaviors and nutrition according to age progression. Young workers, such as nurses, perform in-hive tasks and consume protein-rich pollen, while older workers (foragers) leave the colony to search for food, and consume carbohydrate-rich nectar. These environmentally stimulated events involve transcriptional and DNA epigenetic marks alterations in worker tissues. However, post-transcriptional RNA modifications (epitranscriptomics) are still poorly explored in bees. We investigated the transcriptional profiles of m6A and m5C RNA methyltransferases in the brain and fat body of adult workers of 1) different ages and performing different tasks [nurses of 8 days-old (N-8D) and foragers of 29 days-old (F-29D), sampled from wild-type colonies], and 2) same-aged young workers caged in an incubator and treated with a pollen-rich [PR] or a pollen-deprived [PD] diet for 8 days. In the brain, METTL3, DNMT2, NOP2, NSUN2, NSUN5, and NSUN7 genes increased expression during adulthood (from N-8D to F-29D), while the opposite pattern was observed in the fat body for METTL3, DNMT2, and NSUN2 genes. Regarding diet treatments, high expression levels were observed in the brains of the pollen-deprived group (DNMT2, NOP2, and NSUN2 genes) and the fat bodies of the pollen-rich group (NOP2, NSUN4, and NSUN5 genes) compared to the brains of the PR group and the fat bodies of the PD group, respectively. Our data indicate that RNA epigenetics may be an important regulatory layer in the development of adult workers, presenting tissue-specific signatures of RNA methyltransferases expression in response to age, behavior, and diet content.
ECM-transmitted shear stress induces apoptotic cell extrusion in early breast gland development
Authors: Friedland F. , Babu S. , Springer R. , Konrad J. , Herfs Y. , Gerlach S. , Gehlen J. , Krause H.-J. , De Laporte L. , Merkel R. , Noetzel E. 
Published: August 2022

Epithelial cells of human breast glands are exposed to various mechanical ECM stresses that regulate tissue development and homeostasis. Mechanoadaptation of breast gland tissue to ECM-transmitted shear stress remained poorly investigated due to the lack of valid experimental approaches. Therefore, we created a magnetic shear strain device that enabled, for the first time, to analyze the instant shear strain response of human breast gland cells. MCF10A-derived breast acini with basement membranes (BM) of defined maturation state and basoapical polarization were used to resemble breast gland morphogenesis in vitro. The novel biophysical tool was used to apply cyclic shear strain with defined amplitudes (≤15%, 0.2 Hz) over 22 h on living spheroids embedded in an ultrasoft matrix (&lt;60 Pa). We demonstrated that breast spheroids gain resistance to shear strain, which increased with BM maturation and basoapical polarization. Most intriguingly, poorly developed spheroids were prone to cyclic strain-induced extrusion of apoptotic cells from the spheroid body. In contrast, matured spheroids were insensitive to this mechanoresponse—indicating changing mechanosensing or mechanotransduction mechanisms during breast tissue morphogenesis. Together, we introduced a versatile tool to study cyclic shear stress responses of 3D cell culture models. It can be used to strain, in principle, all kinds of cell clusters, even those that grow only in ultrasoft hydrogels. We believe that this approach opens new doors to gain new insights into dynamic shear strain-induced mechanobiological regulation circuits between cells and their ECM.
10−7 M genistein partially alleviates 10−7 M MEHP unfavorable effects in a new modified fetal rat testis culture system
Authors: Zhang Tong-Dian , Ma Yu-Bo , Gao Ming , Li He-Cheng , Wang Zi-Ming , Chong Tie , Zhang Lian-Dong 
Published: August 2022

Background: Recent studies revealed that some common endocrine-disrupting chemicals (EDCs) including phthalates and phytoestrogens may exhibit low-dose effects properties. However, how low dose of these EDCs and their mixture would affect fetal rat testis development still needs further investigation. Moreover, testis organ culture system also needs further modification to provide an effective tool for ex vivo EDCs study.Methods: We firstly modified the agarose organ culture system, in which fetal rat testes were cultured for 4 days (d1 to d4) on agarose gels held by Millicell inserts. Then we used the modified agarose culture system to study the combined effects of multiple EDCs exposure. 15.5 dpc fetal rat testes were isolated and treated with vehicle, MEHP (0.1 μmol/L), GEN (0.1 μmol/L) or MEHP (0.1 μmol/L) + GEN (0.1 μmol/L). Parameters concerning testicular cell development and function were evaluated, trying to gain insight into the early molecular events after multiple EDCs exposure.Results: The development of somatic, germ cells and seminiferous tubule in 15.5 dpc fetal rat testis was better sustained in the modified agarose culture system. Based on the modified system, we found that MEHP at 0.1 μmol/L induced alterations in gonocyte markers, antioxidative enzyme activity as well as transient reduction of testosterone production, accompanied by mitochondria swelling in gonocytes and Sertoli cells. No obvious morphological and histological alterations were observed in all treated groups. However, coadministration of genistein at 0.1 μmol/L partially alleviated MEHP-induced fetal testis damage ex vivo through enhancement of antioxidative action. MEHP at low dose still showed weak endocrine disrupting properties but did not exhibit typical low-dose effects.Conclusion: Our findings indicated that the modified agarose culture system could better mimic testicular microenvironment without obvious hypoxic cell damage. Furthermore, low dose of MEHP induced mild disruption to fetal testis development, cotreatment of genistein at low dose attenuated MEHP induced fetal testis injuries in part by balancing redox state, indicating that low dose of genistein may partially protect fetal testis from phthalates induced injury.
Miniaturized microfluidic-based nucleic acid analyzer to identify new biomarkers of biopsy lung cancer samples for subtyping
Authors: Lin Xue , Bo Zi-Hao , Lv Wenqi , Zhou Zhanping , Huang Qin , Du Wenli , Shan Xiaohui , Fu Rongxin , Jin Xiangyu , Yang Han , Su Ya , Jiang Kai , Guo Yuchen , Wang Hongwu , Xu Feng , Huang Guoliang 
Published: August 2022

Identifying new biomarkers is necessary and important to diagnose and treat malignant lung cancer. However, existing protein marker detection methods usually require complex operation steps, leading to a lag time for diagnosis. Herein, we developed a rapid, minimally invasive, and convenient nucleic acid biomarker recognition method, which enabled the combined specific detection of 11 lung cancer typing markers in a microliter reaction system after only one sampling. The primers for the combined specific detection of 11 lung cancer typing markers were designed and screened, and the microfluidic chip for parallel detection of the multiple markers was designed and developed. Furthermore, a miniaturized microfluidic-based analyzer was also constructed. By developing a microfluidic chip and a miniaturized nucleic acid analyzer, we enabled the detection of the mRNA expression levels of multiple biomarkers in rice-sized tissue samples. The miniaturized nucleic acid analyzer could detect ≥10 copies of nucleic acids. The cell volume of the typing reaction on the microfluidic chip was only 0.94 μL, less than 1/25 of that of the conventional 25-μL Eppendorf tube PCR method, which significantly reduced the testing cost and significantly simplified the analysis of multiple biomarkers in parallel. With a simple injection operation and reverse transcription loop-mediated isothermal amplification (RT-LAMP), real-time detection of 11 lung cancer nucleic acid biomarkers was performed within 45 min. Given these compelling features, 86 clinical samples were tested using the miniaturized nucleic acid analyzer and classified according to the cutoff values of the 11 biomarkers. Furthermore, multi-biomarker analysis was conducted by a machine learning model to classify different subtypes of lung cancer, with an average area under the curve (AUC) of 0.934. This method shows great potential for the identification of new nucleic acid biomarkers and the accurate diagnosis of lung cancer.
Green synthesized silver nanoparticles: Optimization, characterization, antimicrobial activity, and cytotoxicity study by hemolysis assay
Authors: Liaqat Nida , Jahan Nazish , Khalil-ur-Rahman  , Anwar Tauseef , Qureshi Huma 
Published: August 2022

Green nanotechnology has emerged as a viable option for the production of nanoparticles. The purpose of the current investigation was to synthesize silver nanoparticles (AgNPs) using Eucalyptus camaldulensis and Terminalia arjuna extracts, as well as their combinations, as green reducing and capping agents. The parameters (concentration of silver nitrate solution and plant extract, time, pH, and temperature) were optimized for maximal yields, regulated size, and stability of silver nanoparticles. The ultraviolet–visible spectrophotometer (UV-Vis) and the surface plasmon resonance band (SPR) were used to validate the synthesis of AgNPs. The size, shape, and stability of nanoparticles were assessed using a zeta analyzer and a scanning electron microscope (SEM). The biomolecules responsible for the reduction of silver ion (Ag+) and the stability of silver nanoparticles generated with the plant extracts were identified using Fourier-transform infrared spectroscopy (FTIR). The agar-well diffusion method was used to test the antimicrobial activity of biosynthesized nanoparticles against Bacillus subtilis, Staphylococcus aureus, Pasteurella multocida, and Escherichia coli. When 1 mM of silver nitrate (AgNO3) was added to plant extracts and incubated for 60 min at 75°C in a neutral medium, maximum nanoparticles were produced. Biosynthesized silver nanoparticles were stable, spherical, and monodispersed according to zeta potential and scanning electron microscopy. Silver nanoparticles synthesized with combination 2 and T. arjuna showed the highest zone of inhibition (16 mm) against B. subtilis while combination 3 showed the largest zone of inhibition against S. aureus (17 ± 0.8). It was concluded that greenly produced silver nanoparticles showed good antibacterial activity while causing negligible cytotoxicity.