bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of intra‐BLA overexpression of IGF‐1 on the expression of a contextual fear memory trace
Authors: Champarini Leandro Gabriel , Herrera Macarena Lorena , Comas Mutis Ramiro Gabriel , Espejo Pablo Javier , Molina Victor Alejandro , Calfa Gastón Diego , Hereñú Claudia Beatriz 
Published: August 2022

AbstractGrowth factors, such as insulin‐like growth factor 1 (IGF‐1), among others are known for their critical involvement in learning and memory processes. IGF‐1 regulates cognitive functions, synapse density, neurotransmission, and adult neurogenesis and induces structural and synaptic plasticity‐specific changes. Although IGF‐1 has been suggested to participate in different memory processes, its role in memories associated with negative emotional experiences still remains to be elucidated. The principal aim of the present study was to test whether IGF‐1 overexpression using adenoviral vectors in basolateral amygdala (BLA) influences both the expression and formation of contextual fear memory, as well as the hippocampal structural plasticity associated with such memory trace. We found that IGF‐1 overexpression promotes the formation and expression of a specific contextual fear memory trace, and such effect persisted at least 7 days after recall. Moreover, the overexpression of this growth factor in BLA upregulates the activation of the ERK/MAPK pathway in this brain structure. In addition, intra‐BLA IGF‐1 overexpression causes dorsal hippocampus (DH) structural plasticity modifications promoting changes in the proportion of mature dendritic spines in the CA1 region, after a weak conditioning protocol. The present findings contribute to the knowledge underlying BLA‐DH trace memory of fear and reveal important new insights into the neurobiology and neurochemistry of fear acquisition modulated by IGF‐1 overexpression. The understanding of how IGF‐1 modulates the formation of a fear contextual trace may pave the way for the development of novel therapeutic strategies focused on fear, anxiety, and trauma‐related disorders.
Association of cesarean section with risk of childhood leukemia: A meta‐analysis from an observational study
Authors: Yang Yingzhu , Yu Chiyuan , Fu Rongrong , Xia Shishan , Ni Haixiang , He Yujing , Zhu Keying , Sun Qian 
Published: August 2022

AbstractRecent studies suggest that children born via cesarean section (CS) are predisposed to immune‐mediated diseases later in life. The association between CS and childhood leukemia was investigated in this meta‐analysis of observational studies. Two researchers independently searched PubMed, Web of Science, Embase, and Cochrane Library for literature on the association between CS and childhood leukemia before February 2022. And pooled odds ratios (OR) with 95% confidence intervals (CI) were calculated to determine the link between CS and childhood leukemia. The preliminary search resulted in 1321 articles and 16 articles were finally included after screening. The primary outcome was the risk of leukemia in children born via CS versus those born vaginally. The results revealed that having a CS was associated with an increased risk of childhood leukemia compared to having vaginal section (VS) (OR = 1.07, 95% CI: 1.02–1.13, p = 0.01), especially for acute lymphoblastic leukemia (ALL) (OR = 1.09, 95% CI: 1.03–1.16, p = 0.004). Children delivered via elective CS had a higher risk of ALL (OR = 1.18, 95% CI: 1.07–1.31, p = 0.001), but emergency CS did not. It is worth noting that neither emergency CS nor elective CS were found to be associated with acute myeloid leukemia. Compared to VS, CS increased the risk of leukemia in children, with elective CS significantly increasing ALL risk.
Standardized reporting of amnion and amnion/chorion allograft data for wound care
Authors: Sabol Taylor J. , Tran Grace S. , Matuszewski Jason , Weston Wendy W. 
Published: August 2022

AbstractBackgroundThe favorable biological and mechanical properties of the most common components of the placenta, the amnion and chorion, have been explored for regenerative medical indications. The use of the combination of amnion and chorion has also become very popular. But, published data from placental tissues in their final, useable form is lacking. During treatment with membrane product, the tissue is usually sterile, intact and laid on a wound or treatment area. The factors available to the treatment area from the applied product need to be elucidated and presented in a relatable form. Current reporting for eluted growth factor results are typically expressed per milliliter, which is not informative with respect to the area of tissue covered by the actual membrane and may differ among techniques.MethodsTo address this inconsistency, amnion or amnion/chorion were isolated from human placentas and processed by a proprietary procedure. The final dry, sterilized product was evaluated for structural components and growth factor elution. Growth factors were quantified by multiplex panels and ELISAs and the values normalized to specific area and elution volume of finished product. This information allows extrapolation to all membrane sizes and affords cross‐study comparisons.ResultsAnalysis of membrane supernatants show that dehydrated, sterilized amnion and amnion/chorion elute factors that are conducive to wound healing, which are available to recipient tissues. Importantly, these measurable factors eluted from dehydrated, sterilized membranes can be reported as a function of available factors per square centimeter of tissue.ConclusionsThe standardized characterization of dehydrated, sterilized amnion and amnion/chorion as delivered to recipient tissues permits understanding and comparison of the products across various graft sizes, types, and eluate volumes. Further, reporting this data as a function of cm2 of dehydrated tissue allows extrapolation by independent scientists and clinicians.
Using theory to guide exploratory network analyses
Authors: Lass Alisson N. S. , Jordan D. Gage , Winer E. Samuel 
Published: August 2022

AbstractThe use of exploratory network analysis has increased in psychopathology research over the past decade. A benefit of exploratory network analysis is the wealth of information it can provide; however, a single analysis may generate more inferences than what can be discussed in one manuscript (e.g., centrality indices of each node). This necessitates that authors choose which results to discuss in further detail and which to omit. Without a guide for this process, the likelihood of a biased interpretation is high. We propose that the integration of theory throughout the research process makes the interpretation of exploratory networks more manageable for the researcher and more likely to result in an interpretation that advances science. The goals of this paper are to differentiate between exploratory and confirmatory network analyses, discuss the utility of exploratory work, and provide a practical framework that uses theory as a guide to interpret exploratory network analyses.
Development of modified graphite electrodes with nanoparticles and their application in the quantification of sucrose
Authors: dos Santos Luana Dalagrana , Ribeiro Vítor Antônio Vitale , de Oliveira Byanca Pereira Moreira , Killner Mario Henrique Montazzolli , Gonçalves Odinei Hess , Bona Evandro 
Published: August 2022

AbstractBACKGROUNDDeveloping fast and reliable methods with little sample preparation has become increasingly important for process monitoring and control. Electrochemical methods stand out for their low cost and instrumental simplicity. Furthermore, electrodes with nanoparticles can improve the method's sensitivity and specificity. This study developed carbon paste electrodes modified with nanoparticles of copper and iron. In addition, cyclic voltammetry was performed using a low‐cost potentiostat designed for a Raspberry Pi single‐board computer. As a proof of concept, the constructed electrodes were tested with ferrocyanide and applied to quantify sucrose, and their performance was compared to that of the glassy carbon electrode.RESULTSThe nanoparticles were successfully synthesized, as confirmed by morphology and physicochemical characterization. Based on the adjusted R2, the electrode made with the addition of copper oxide nanoparticles has a similar performance to the commercial glassy carbon electrode. All electrodes' root mean square error (RMSE) values were approximately 10−4 mol L−1. The lowest values for the inverse of the analytical sensitivity for sucrose were obtained for the unmodified carbon paste electrode (9.54 × 10−6 mol L−1) and copper oxide nanoparticles electrode (5.30 × 10−6 mol L−1). These electrodes also had the lowest limits of detection (10−5 mol L−1) and, for the limit of quantification, all electrodes had similar values (10−2 mol L−1).CONCLUSIONIn general, superior performance of carbon paste electrodes was observed concerning the commercial glassy carbon electrodes. Compared to previous studies, the developed electrodes showed good sensitivity and the low cost of the electrochemical system stands out as the main advantage. © 2022 Society of Chemical Industry (SCI).
In vitro-induced Foxp3+CD8+ regulatory T cells suppress allergic IgE response in the gut
Authors: Giri Shilpi , Meitei Heikrujam Thoihen , Sonar Sandip Ashok , Shaligram Saumitra , Lal Girdhari 
Published: August 2022

AbstractSeveral subsets of CD8+ T cells are known to have a suppressive function in different tissues and diseases in mice and humans. Due to the lack of a consensus on the phenotype of regulatory CD8+ T cells and very low frequency in the body, its clinical use as adoptive cellular therapy has not advanced much. In the present work, using DNA methyltransferase inhibitor 5-Aza-2′-deoxycytidine (Aza), we efficiently and stably differentiated naïve CD8+ T cells (CD8+CD25–CD44– cells) into the CD8+Foxp3+ regulatory CD8+ T cells (CD8 Tregs). We also generated OVA peptide257-264-specific CD8+Foxp3+ Tregs. Compared with activated CD8 T cells, Aza plus TGF-β-induced CD8+Foxp3+ Tregs showed significantly increased surface expression of CD39, CD73, CD122, CD62L, and CD103, and secreted TGF-β and suppressed the proliferation of effector CD4+ T cells. Interestingly, CD8+Foxp3+ Tregs exhibited low expression of perforin and granzyme required for cytotoxic function. Analysis of chemokine receptors showed that TGF-β + Aza induced CD8+Foxp3+ Tregs expressed gut-tropic chemokine receptors CCR6 and CCR9, and chemokine receptors CCR7 and CXCR3 required for mobilization into the spleen, lymph nodes, and gut-associated lymphoid tissues. Adoptive transfer of induced CD8+Foxp3+ Tregs restored cholera toxin-induced breakdown of oral tolerance to OVA by regulating OVA-specific IgE and IgG1. Altogether, we showed an efficient method to generate antigen-specific CD8+Foxp3+ Tregs, and the adoptive transfer of these cells induces oral tolerance by suppressing allergic response and maintaining intestinal homeostasis.
The prevalence of inherited metabolic disorders in Estonian population over 30 years: A significant increase during study period
Authors: Tiivoja Elis , Reinson Karit , Muru Kai , Rähn Kristi , Muhu Kristina , Mauring Laura , Kahre Tiina , Pajusalu Sander , Õunap Katrin 
Published: August 2022

AbstractInherited metabolic disorders (IMD) are a group of hereditary diseases wherein the impairment of a biochemical pathway is intrinsic to the pathophysiology of the disease. Estonia's small population and nationwide digitalised healthcare system make it possible to perform an epidemiological study that covers the whole population. A study was performed in Tartu University Hospital, which is the only tertiary care unit in Estonia for diagnosing patients with IMD, to define the prevalence and live birth prevalence of IMDs and the effectiveness of new diagnostic methods on the diagnosis of IMD. During the retrospective study period from 1990 to 2017, 333 patients were diagnosed with IMD. Statistical analysis showed a significant increase in IMD diagnoses per year from 0.47 to 2.51 cases per 100 000 persons (p < 0.0001) during the study period. Live birth prevalence of IMD in Estonia was calculated to be 41.52 cases per 100 000 live births. The most frequently diagnosed IMD groups were disorders of amino acid metabolism, disorders of complex molecule degradation, mitochondrial disorders, and disorders of tetrapyrrole metabolism. Phenylketonuria was the most frequently diagnosed disorder of all IMD (21.6%). Our results correlated well with data from other developed countries and, along with high birth prevalence, add confidence in the effectiveness of our diagnostic yield. Implementation of new diagnostic methods during study period may largely account for the significant increase in the number of IMD diagnoses per year. We conclude that the implementation of new diagnostic methods continues to be important and contributes to better diagnosis of rare diseases.
Reconstruction of molecular evolution of human influenza A H1N1/2009 virus in Iran and neighboring countries
Authors: Ghalekhani Nima , Bokaie Saied , Eybpoosh Sana , Akbarein Hesameddin , Sharifi Hamid 
Published: August 2022

AbstractIn this study, the time and path of transmission of H1N1 serotype influenza A viruses in Iran and neighboring countries have been investigated by using Bayesian phylogeography analysis on the sequences extracted from the gene bank. We obtained all hemagglutinin (HA) and neuraminidase (NA) nucleotide sequences of influenza H1N1 available up to December 25, 2020, from Iran and its neighboring countries (i.e., Pakistan, Afghanistan, Turkmenistan, Armenia, Azerbaijan, Turkey, and Iraq). We also performed a Bayesian Markov chain Monte Carlo method to infer the evolutionary dynamic and the most recent common ancestor for the HA and NA sequences. Based on the extracted sequences, the age of emergence of H1N1 influenza virus serotype was older in Iran compared to neighboring countries, and with some degree of uncertainty, it seems Tehran had a key role and epicenter of transmission to other cities within Iran. The mean time of the most recent common ancestor of H1N1 viruses was 1989 (95% HPD: 1980–1994) for HA and NA as well. Along with ordinary measures like resource management, diagnostic approaches, and preparedness to fight against viruses that were in place, continuous monitoring, and screening of H1N1 serotype influenza virus in the country, especially by implementation of feasible, effective, and innovative measures at borderline should be initiated and identified gaps and shortage that should be a priority for virus control. It is also important for countries to have a regional monitoring program in addition to internal monitoring programs, as well as to start a virus molecular care program.
Comparison of pregabalin with magnesium sulfate in the prevention of remifentanil‐induced hyperalgesia in patients undergoing rhinoplasty: A randomized clinical trial
Authors: Hadavi Seyed Mohammad Reza , Eghbal Mohammad Hossein , Kaboodkhani Reza , Alizadeh Narges , Sahmeddini Mohammad Ali 
Published: August 2022

AbstractObjectiveRemifentanil is usually used for controlled hypotension during rhinoplasty under general anesthesia (G/A). One of the complications of the remifentanil is postoperative hyperalgesia. In this study, we compare the effectiveness of pregabalin with that of Mg sulfate in postoperative remifentanil‐induced hyperalgesia prevention.MethodsIn this prospective, randomized, double‐blinded placebo‐controlled trial, 105 patients who candidates rhinoplasty with G/A were enrolled and randomly allocated into three groups. Patients in group A received 300 mg pregabalin before anesthesia. They received physiologic saline infusion during the perioperative period. Those in group B received placebo capsules before anesthesia and intravenous Mg sulfate 30 mg/kg during the perioperative period. Those in group C received a placebo capsule before anesthesia and normal intravenous saline during the operation. Then, pain severity, sedation score, postoperative nausea and vomiting (PONV) were assessed and compared.ResultsIn the Mg sulfate and placebo group, the mean numerical rating scale in the postoperative period was higher compared to the pregabalin group (p < .001). The mean total amount of morphine requirement, meanwhile the 24 h post‐operation, was significantly decreased in the pregabalin group compared to the other groups (p < .001). Participants in the pregabalin group had less PONV compared to those in the pregabalin and placebo groups (p = .015).ConclusionsIn patients undergoing G/A with remifentanil for rhinoplasty, preoperative 300 mg pregabalin could effectively prevent not only remifentanil‐induced hyperalgesia but also PONV.Level of evidence1b.
AAPM WGTG51 Report 374: Guidance for TG‐51 reference dosimetry
Authors: Muir Bryan , Culberson Wesley , Davis Stephen , Kim Grace Gwe‐Ya , Lee Sung‐Woo , Lowenstein Jessica , Renaud James , Sarfehnia Arman , Siebers Jeffrey , Tantôt Laurent , Tolani Naresh 
Published: August 2022

AbstractPractical guidelines that are not explicit in the TG‐51 protocol and its Addendum for photon beam dosimetry are presented for the implementation of the TG‐51 protocol for reference dosimetry of external high‐energy photon and electron beams. These guidelines pertain to: (i) measurement of depth‐ionization curves required to obtain beam quality specifiers for the selection of beam quality conversion factors, (ii) considerations for the dosimetry system and specifications of a reference‐class ionization chamber, (iii) commissioning a dosimetry system and frequency of measurements, (iv) positioning/aligning the water tank and ionization chamber for depth ionization and reference dose measurements, (v) requirements for ancillary equipment needed to measure charge (triaxial cables and electrometers) and to correct for environmental conditions, and (vi) translation from dose at the reference depth to that at the depth required by the treatment planning system. Procedures are identified to achieve the most accurate results (errors up to 8% have been observed) and, where applicable, a commonly used simplified procedure is described and the impact on reference dosimetry measurements is discussed so that the medical physicist can be informed on where to allocate resources.
Sheared two‐dimensional radiofrequency excitation for off‐resonance robustness and fat suppression in reduced field‐of‐view imaging
Authors: Barlas Bahadir Alp , Bahadir Cagla Deniz , Kafali Sevgi Gokce , Yilmaz Ugur , Saritas Emine Ulku 
Published: August 2022

PurposeTwo‐dimensional (2D) echo‐planar radiofrequency (RF) pulses are widely used for reduced field‐of‐view (FOV) imaging in applications such as diffusion‐weighted imaging. However, long pulse durations render the 2D RF pulses sensitive to off‐resonance effects, causing local signal losses in reduced‐FOV images. This work aims to achieve off‐resonance robustness for 2D RF pulses via a sheared trajectory design.Theory and MethodsA sheared 2D RF pulse design is proposed to reduce pulse durations while covering identical excitation k‐space extent as a standard 2D RF pulse. For a given shear angle, the number of sheared trajectory lines is minimized to obtain the shortest pulse duration, such that the excitation replicas are repositioned outside the slice stack to guarantee unlimited slice coverage. A target fat/water signal ratio of 5% is chosen to achieve robust fat suppression.ResultsSimulations, imaging experiments on a custom head and neck phantom, and in vivo imaging experiments in the spinal cord at 3 T demonstrate that the sheared 2D RF design provides significant improvement in image quality while preserving profile sharpnesses. In regions with high off‐resonance effects, the sheared 2D RF pulse improves the signal by more than 50% when compared to the standard 2D RF pulse.ConclusionThe proposed sheared 2D RF design successfully reduces pulse durations, exhibiting significantly improved through‐plane off‐resonance robustness, while providing unlimited slice coverage and high fidelity fat suppression. This method will be especially beneficial in regions suffering from a variety of off‐resonance effects, such as spinal cord and breast.
Health consequences of obesity and projected future obesity health burden in China
Authors: Sun Xiaomin , Yan Alice Fang , Shi Zumin , Zhao Bingtong , Yan Na , Li Ke , Gao Liwang , Xue Hong , Peng Wen , Cheskin Lawrence J. , Wang Youfa 
Published: August 2022

AbstractObjectiveThis study examined the effects of overweight/obesity on mortality and morbidity outcomes and the disparities, time trends, and projected future obesity health burden in China.MethodsCohort studies that were conducted in China and published in English or Chinese between January 1, 1995, and July 31, 2021, were systematically searched. This study focused on overweight/obesity, type 2 diabetes mellitus (T2DM), hypertension, cardiovascular diseases, metabolic syndrome, cancers, and chronic kidney disease.ResultsA total of 31 cohorts and 50 cohort studies reporting on mortality (n = 20) and morbidities (n = 30) associated with obesity met study inclusion criteria. Overall, BMI was nonlinearly (U‐shaped) associated with all‐cause mortality and linearly associated with risks of T2DM, cardiovascular diseases, hypertension, cancer, metabolic syndrome, and chronic kidney disease. In 2018, among adults, the prevalence of overweight/obesity, hypertension, and T2DM was 51.2%, 27.5%, and 12.4%, respectively. Their future projected prevalence would be 70.5%, 35.4%, and 18.5% in 2030, respectively. The projected number of adults having these conditions would be 810.65 million, 416.47 million, and 217.64 million, respectively. The urban‐rural disparity in overweight/obesity prevalence was projected to shrink and then reverse over time.ConclusionsThe current health burden of obesity in China is high and it will sharply increase in coming years and affect population groups differently. China needs to implement vigorous interventions for obesity prevention and treatment.
Preliminary study of particleboards manufactured with pine cones and Eucalyptus globulus capsules with a bio‐based polyurethane adhesive
Authors: Wechsler‐Pizarro Andrea , Núñez‐Decap Mario 
Published: August 2022

AbstractThe following research aims to evaluate the feasibility of manufacturing particleboards based in Pinus radiata D. DON (PC) and Eucalyptus globulus (EC) capsules bonded with a natural oil polyurethane adhesive (PU). The particles of PC and EC were ground and dried. Particleboards were manufactured with PC and EC bonded with a natural oil‐based polyurethane adhesive (PC–PU and EC–PU). Boards composed of radiata pine wood and bio based polyurethane adhesive (RP–PU) and with radiata pine wood bonded with urea formaldehyde resin (RP–UF) were manufactured as reference samples. The physical and mechanical properties and formaldehyde emissions were evaluated according to international standards. The superficial morphology of the particleboards was studied through optical microscopy (OP) and scanning electron microscopy (SEM). The novel particleboards showed high resistance to moisture, low thickness swelling and a high internal bond strength, when compared with particleboards manufactured with the radiata pine wood particleboards bonded with PU and UF. Flexural properties in the new particleboards were slightly lower than the flexural properties of the reference samples RP–PU and RP–UF, since the distribution and geometry of the particles were different. The results of this research validate the use of P. radiata D. DON cones and E. globulus capsules, bonded with a bio‐based polyurethane adhesive, for the manufacture of novel particleboards. This research opens the possibility of an alternative use for the pine cones and Eucalyptus capsules as a new prime source for the manufacture of particleboards and other composite materials.