bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brief interventions for alcohol use disorders in low- and middle-income countries: barriers and potential solutions
Authors: Nadkarni Abhijit , Bhatia Urvita , Bedendo Andre , de Paula Tassiane Cristine Santos , de Andrade Tostes Joanna Gonçalves , Segura-Garcia Lidia , Tiburcio Marcela , Andréasson Sven 
Published: August 2022

AbstractGlobal alcohol consumption and harmful use of alcohol is projected to increase in the coming decades, and most of the increase will occur in low- and middle-income countries (LMICs); which calls for cost-effective measures to reduce alcohol exposure in these countries. One such evidence based measure is screening and brief intervention (BI) for alcohol problems. Some of the characteristics of BI make them a particularly appealing choice of interventions in low-resource settings. However, despite evidence of effectiveness, implementation of BI in LMICs is rare. In this paper we discuss barriers to implementation of BI in LMICs, with examples from Latin America and India. Key barriers to implementation of BI in LMICs are the lack of financial and structural resources. Specialized services for alcohol use disorders are limited or non-existent. Hence primary care is often the only possible alternative to implement BI. However, health professionals in such settings generally lack training to deal with these disorders. In our review of BI research in these countries, we find some promising results, primarily in countries from Latin America, but so far there is limited research on effectiveness. Appropriate evaluation of efficacy and effectiveness of BI is undermined by lack of generalisability and methodological limitations. No systematic and scientific efforts to explore the implementation and evaluation of BI in primary and community platforms of care have been published in India. Innovative strategies need to be deployed to overcome supply side barriers related to specialist manpower shortages in LMICs. There is a growing evidence on the effectiveness of non-specialist health workers, including lay counsellors, in delivering frontline psychological interventions for a range of disorders including alcohol use disorders in LMICs. This paper is intended to stimulate discussion among researchers, practitioners and policy-makers in LMICs because increasing access to evidence based care for alcohol use disorders in LMICs would need a concerted effort from all these stakeholders.
Incorporating immersive learning into biomedical engineering laboratories using virtual reality
Authors: Wilkerson Megan , Maldonado Vitali , Sivaraman Srikanth , Rao Raj R. , Elsaadany Mostafa 
Published: August 2022

AbstractBackgroundThe Covid-19 pandemic caused a sudden shift towards remote learning, moving classes to online formats. Not exempt from this switch, laboratory courses traditionally taught in-person were also moved to remote methods, costing students the opportunity to learn these skills hands-on. In order for instructors to provide course materials effectively and engagingly, non-traditional methods should be explored.Virtual reality (VR) has become more accessible in recent years. VR simulations have been used for many years as educational tools in high-risk settings such as flight or medical simulations. Immersive VR videos implemented in a remote laboratory course could provide the students with an engaging and suitable learning experience.To test the effectiveness of VR videos as a tool for remote education, VR videos of the laboratory component of a Biomolecular Engineering course were provided to students. A survey was distributed for students to self-report their experience with the videos. The survey contained quantitative and qualitative ratings of VR as an educational tool.ResultsThe survey showed that students (~ 89% strongly agree or agree) believed the videos provided the opportunity to work at their own pace and were an appropriate length. While ~ 74% of students said that the videos provided enough information to understand the tasks, a small percentage felt that the videos improved their retention (~ 16%) and understanding (~ 9%) of the course material. About 28% of the students responded positively when asked about how VR videos improved their engagement with the material. ~ 30% reported confidence in applying the skills learned in the videos in the future and ~ 43% believe the VR videos were an acceptable alternative to in-person labs. Two-thirds of students reported feeling some form of discomfort while viewing the VR videos and 54% reported not using the headset for the videos and using the 3D video feature instead.ConclusionsAs many students reported the videos containing appropriate information, the content of the videos was not an issue. A combination of improved camera quality with motion stability, more comfortable headsets, and a reduction in editing issues could greatly improve the quality and effectiveness of VR videos.
Suppression of VEGFD expression by S-nitrosylation promotes the development of lung adenocarcinoma
Authors: He Qiangqiang , Qu Meiyu , Shen Tingyu , Xu Yana , Luo Jiahao , Tan Dan , Xu Chengyun , Barkat Muhammad Qasim , Zeng Ling-Hui , Wu Ximei 
Published: August 2022

AbstractBackgroundVascular endothelial growth factor D (VEGFD), a member of the VEGF family, is implicated in angiogenesis and lymphangiogenesis, and is deemed to be expressed at a low level in cancers. S-nitrosylation, a NO (nitric oxide)-mediated post-translational modification has a critical role in angiogenesis. Here, we attempt to dissect the role and underlying mechanism of S-nitrosylation-mediated VEGFD suppression in lung adenocarcinoma (LUAD).MethodsMessenger RNA and protein expression of VEGFD in LUAD were analyzed by TCGA and CPTAC database, respectively, and Assistant for Clinical Bioinformatics was performed for complex analysis. Mouse models with urethane (Ure)–induced LUAD or LUAD xenograft were established to investigate the role of S-nitrosylation in VEGFD expression and of VEGFD mutants in the oncogenesis of LUAD. Molecular, cellular, and biochemical approaches were applied to explore the underlying mechanism of S-nitrosylation-mediated VEGFD suppression. Tube formation and wound healing assays were used to examine the role of VEGFD on the angiogenesis and migration of LUAD cells, and the molecular modeling was applied to predict the protein stability of VEGFD mutant.ResultsVEGFD mRNA and protein levels were decreased to a different extent in multiple primary malignancies, especially in LUAD. Low VEGFD protein expression was closely related to the oncogenesis of LUAD and resultant from excessive NO-induced VEGFD S-nitrosylation at Cys277. Moreover, inhibition of S-nitrosoglutathione reductase consistently decreased the VEGFD denitrosylation at Cys277 and consequently promoted angiogenesis of LUAD. Finally, the VEGFDC277Smutant decreased the secretion of mature VEGFD by attenuating the PC7-dependent proteolysis and VEGFDC277Smutant thus reversed the effect of VEGFD on angiogenesis of LUAD.ConclusionLow-expression of VEGFD positively correlates with LUAD development. Aberrant S-nitrosylation of VEGFD negates itself to induce the tumorigenesis of LUAD, whereas normal S-nitrosylation of VEGFD is indispensable for its secretion and repression of angiogenesis of LUAD.
Atorvastatin for reduction of 28-day mortality in hospitalized COVID-19 patients: study protocol for a randomized, double-blinded, placebo-controlled, clinical trial
Authors: Emara Moataz Maher , Elsawy Neamat Hamdy , Abdelaaty Kholoud M. , Elhamaky Amal Salah , Eltahan Naglaa Hamdi 
Published: August 2022

Abstract
Background
Although mass vaccination has reduced the severity of COVID-19, mortality is still high among hospitalized patients. Being a sepsis-like disease, an anti-inflammatory drug as atorvastatin would reduce mortality and severity in COVID-19.

Methods
We designed a randomized clinical trial that recruited 220 COVID-19 patients admitted in the COVID-19 isolation hospital at Mansoura University, Egypt. One hundred ten cases were assigned to receive 40 mg atorvastatin once daily for 28 days, and 110 were assigned to receive placebo. Delta Pharm company supported the study with the drug and the placebo, which mimics the drug as regards the drug package, the tablet color, consistency, and size. All patients received the standard treatment as per the hospital protocol. The Institutional Review Board approval and the informed consent from all participants were obtained.
The primary outcome is the 28-day all-cause mortality. Additionally, we will collect the in-hospital mortality, the need for mechanical ventilation, time to clinical improvement, in-hospital thrombo-embolic events, acute kidney injury, and the hospital and the intensive care duration of stay. We plan to follow the patients up for 6 months for reporting mortality and long-term neurological, psychological, and respiratory consequences.
We will report the un-adjusted 28-mortality using χ2. Then, we will report the adjusted odds ratio with a pre-planned multiple logistic regression model. We will report our results using the point estimate and the 95% confidence interval and the P-value.

Discussion
The additional issue that we would like to discuss is the added workload on the clinicians and the allied healthcare workers who performed research at the time of the pandemic. Therefore, doing research at the pandemic era was, indeed, challenging.

Trial registration
The study was registered at the Clinical Trial Registry (NCT04952350) on July 1st, 2021.
Qualitative interviews to understand health care providers’ experiences of prescribing licensed peanut oral immunotherapy
Authors: Anagnostou Aikaterini , Lawrence Claire , Tilles Stephen A. , Laubach Susan , Donelson Sarah M. , Yassine Mohamed , Nowak-Wegrzyn Anna 
Published: August 2022

Abstract
Objective
This research sought to explore health care providers’ (HCPs) experiences of delivering the first US Food and Drug Administration (FDA) and European Commission (EC) approved peanut oral immunotherapy (peanut OIT; Palforzia). Semi-structured qualitative interviews with HCPs who had initiated treatment with ≥ 3 patients in the first nine months following FDA approval sought to identify challenges faced and successful implementation strategies.

Results
Eight allergists and three nurse practitioners from eight sites based in the United States participated. The HCPs included in this research were motivated to implement this novel treatment, however, entered the process with some reservations. HCPs described how successful implementation of peanut OIT requires them to be thoughtful about their clinic’s abilities to integrate complex, time-consuming treatments into their daily practice. Prior experience of OIT was deemed beneficial, but not essential for implementation and learning from others’ experience was suggested as a way of helping new prescribers overcome perceived and actual implementation challenges. Delivering licensed peanut OIT during the COVID-19 pandemic posed both challenges and unexpected opportunities for implementation. The experiences described have the potential to benefit the wider allergy community by providing practical solutions, successful implementation strategies and opportunities to enhance training and resources.
Association between constipation and the development of asthma: a meta-analysis
Authors: Liu Lu , Zhang Xiangli , Jiang Zhengdong , Wang Guizuo , Wu Hua , Chen Ruilin , Zhang Yongqing , Li Manxiang , Yang Shumei 
Published: August 2022

Abstract
Background
Constipation has been hypothesized to be associated with the increased risk of wheezing or asthma. However, the relation remains a subject of debate. We conducted this meta-analysis to assess whether constipation influences the risk of wheezing/asthma.

Methods
PubMed, Embase, and Web of Science were systematically searched for studies published between 1955 and January 2022. Two reviewers independently extracted data and assessed the quality of each study. Results were pooled using fixed-effects models or random-effects models as appropriate.

Results
In total, 3 original articles with 178,661 participants, which met the criteria, were included in this meta-analysis. Constipation was associated with an increased risk of wheezing/asthma in later life (RR = 2.02, 95% CI = 1.24–3.29, P < 0.01).

Conclusions
The meta-analysis suggests an association between constipation and the subsequent development of wheezing/asthma. Well-designed and highly standardized prospective studies that adequately address concerns for potential confounding factors are required to validate the risk identified in our current meta-analysis.
A review of more than 2000 cases of site-specific pelvic endometriosis rates by MRI: a guide to minimizing under/overdiagnosis non-invasively
Authors: Hajati Azadeh , Hajati Omid 
Published: August 2022

Abstract
Objectives
To statistically study the incidence of endometrioma and deep infiltrating endometriosis (DIE) in various anatomical sites and to illustrate the significance and potential implications for each site. Furthermore, to improve the knowledge of the community for a non-invasive diagnosis alternative to laparoscopy.

Methods
This study includes 2040 patients who had been referred with clinical evidence of pelvic endometriosis. These patients had been examined and undergone transvaginal sonography by the referring gynecologists. The imaging modality used to discover and locate various anatomical locations of involvement was MRI with contrast. Two radiologists with expertise in endometriosis separately assessed the patients' MRIs and highlighted the involved areas.

Results
In total, 79.1% were positive for either endometrioma or DIE. We detected both DIE and ovarian endometrioma in 78.2% of positive cases. Isolated endometrioma or DIE was present exclusively in 13.7% and 8.1% of patients, respectively. Uterosacral ligaments were detected as the most common (73.8%) site of DIE involvements and in 2.9% of cases were the sole affected location. Interestingly, very rare independent involvement of the genitourinary tract was seen in two patients.

Conclusions
In this study, MRI was used to assess the likely involvement sites of endometrioma and DIE, as well as the frequency of incidences in various places and their relationships over a large dataset. Understanding the possibly involved sites, their statistics, and their co-existence can provide radiologists with a roadmap for non-invasive endometriosis diagnosis and treatment planning. These principles should hopefully assist reduce under- and overdiagnosis.
Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration
Authors: Zhou Yang , Zhang Xing-Liao , Lu Shou-Tao , Zhang Ning-Yan , Zhang Hai-Jun , Zhang Jing , Zhang Jun 
Published: August 2022

Abstract
Background
Large area skin trauma has always been a great challenge for both patients and clinicians. Exosomes originating from human adipose-derived mesenchymal stem cells (hADSCs) have been a novel promising cell-free treatment in cutaneous damage repair. Nevertheless, the low retention rate of exosomes post-transplantation in vivo remains a significant challenge in clinical applications. Herein, we purposed to explore the potential clinical application roles of hADSCs-Exos encapsulated in functional PF-127 hydrogel in wound healing.

Methods
hADSCs-Exos were isolated from human hADSCs by ultracentrifugation. An injectable, biocompatible, and thermo-sensitive hydrogel Pluronic F-127 hydrogel was employed to encapsulate allogeneic hADSCs-Exos, and this complex was topically applied to a full-thickness cutaneous wound in mice. On different days post-transplantation, the mice were sacrificed, and the skin tissue was excised for histological and immunohistochemical analysis.

Results
Compared with hADSCs-Exos or PF-127 only, PF-127/hADSCs-Exos complexes enhanced skin wound healing, promoted re-epithelialization, increased expression of Ki67, α-SMA, and CD31, facilitated collagen synthesis (Collagen I, Collagen III), up-regulated expression of skin barrier proteins (KRT1, AQP3), and reduced inflammation (IL-6, TNF-α, CD68, CD206). By using PF-127/hADSCs-Exos complexes, hADSCs-Exos can be administrated at lower doses frequency while maintaining the same therapeutic effects.

Conclusion
Administration of hADSCs-Exos in PF-127 improves the efficiency of exosome delivery, maintains the bioactivity of hADSCs-Exos, and optimizes the performance of hADSCs-Exos. Thus, this biomaterial-based exosome will be a promising treatment approach for the cutaneous rejuvenation of skin wounds.
Ursodeoxycholic acid production by Gibberella zeae mutants
Authors: Kollerov Vyacheslav , Donova Marina 
Published: August 2022

AbstractUrsodeoxycholic acid (UDCA) is a highly demanded pharmaceutical steroid widely used in medicine. An ascomycete Gibberella zeae VKM F-2600 is capable of producing UDCA by 7β-hydroxylation of lithocholic acid (LCA). The present study is aimed at the improvement of the fungus productivity. The original procedures for the protoplast obtaining followed by UV mutagenesis and screening of ketoconazole-resistant mutant clones have been applied. The highest yield of G. zeae protoplasts was obtained when using the mycelium in the active growth phase, ammonium chloride as an osmotic stabilizer and treatment of the fungal cells by the lytic enzymes cocktail from Trichoderma hurzanium. The conditions for effective protoplast regeneration and the UV-mutagenesis were found to provide 6–12% survival rate of the protoplasts with superior number of possible mutations. Three of 27 ketoconazole-resistant mutant clones obtained have been selected due to their increased biocatalytic activity towards LCA. The mutant G. zeae M23 produced 26% more UDCA even at relatively high LCA concentration (4 g/L) as compared with parent fungal strain, and the conversion reached 88% (w/w). The yield of UDCA reached in this study prefers those ever reported. The results contribute to the knowledge on ascomycete mutagenesis, and are of importance for biotechnological production of value added cholic acids.
Graphical Abstract
Effect of cell density on decrease in hydraulic conductivity by microbial calcite precipitation
Authors: Eryürük Kağan 
Published: August 2022

AbstractThe effect of number of cells deposited on decrease in hydraulic conductivity of porous media using CaCO3 precipitation induced by Sporosarcina pasteurii (ATCC 11,859) was examined in columns packed with glass beads in the range of 0.25 mm and 3 mm in diameter. After resting Sporosarcina pasteurii cells were introduced into the columns, a precipitation solution, which consisted of 500 mM CaCl2 and 500 mM urea, was introduced under continuous flow conditions. It was shown that hydraulic conductivity was decreased by formation of microbially induced CaCO3 precipitation from between 8.37 * 10−1 and 6.73 * 10−2 cm/s to between 3.69 * 10−1 and 1.01 * 10−2 cm/s. The lowest hydraulic conductivity was achieved in porous medium consisting of the smallest glass beads (0.25 mm in diameter) using the highest density of cell suspension (OD600 2.25). The number of the deposited cells differed depending on the glass bead size of the columns. According to the experiments, 7 * 10−9 g CaCO3 was produced by a single resting cell. The urease activity, which led CaCO3 precipitation, depended on presence of high number of cells deposited in the column because the nutrients were not included in the precipitation solution and consequently, the amount of CaCO3 precipitated was proportional with the cell number in the column. A mathematical model was also developed to investigate the experimental results, and statistical analysis was also performed.
N6-methyladenosine-mediated CELF2 regulates CD44 alternative splicing affecting tumorigenesis via ERAD pathway in pancreatic cancer
Authors: Lai Shihui , Wang Yan , Li Ting , Dong Yihong , Lin Yihao , Wang Liang , Weng Shangeng , Zhang Xiang , Lin Chengjie 
Published: August 2022

Abstract
Background
Alternative splicing (AS) of genes has been found to affect gene stability, and its abnormal regulation can lead to tumorigenesis. CELF2 is a vital splicing factor to participate in mRNA alternative splicing. Its downregulation has been confirmed to promote the occurrence and development of pancreatic cancer (PC). However, the regulatory role and mechanisms in PC has not been elucidated.

Results
CELF2 was downregulated in PC tissues, which affected tumor TNM stage and tumor size, and low expression of CELF2 indicated a poor prognosis of PC. In vivo and in vitro experiments showed that abnormal expression of CELF2 affected the stemness, apoptosis, and proliferation of PC cells. Furthmore, we also found that CELF2 was targeted by ALKBH5 for m6A modification, leading to CELF2 degradation by YTHDF2. Bioinformatic analysis of AS model based on the TCGA database indicated that CELF2 could target CD44 to form different spliceosomes, thereby affecting the biological behavior of PC cells. The conversion of CD44s to CD44V is the key to tumorigenesis. Transcriptomic analysis was conducted to reveal the mechanism of CELF2-mediated CD44 AS in PC. We found that CELF2-mediated splicing of CD44 led to changes in the level of endoplasmic reticulum stress, further regulating the endoplasmic reticulum-associated degradation (ERAD) signaling pathway, thereby affecting apoptosis and cell stemness. In addition, ERAD signaling pathway inhibitor, EerI, could effectively reverse the effect of CD44 on tumors.

Conclusions
This study indicates that N6-methyladenosine-mediated CELF2 promotes AS of CD44, affecting the ERAD pathway and regulating the biological behavior of PC cells. CELF2 is expected to be a new target for targeted-drug development.
Class of operators related to a $(m,C)$-isometric tuple of commuting operators
Authors: Al Dohiman Abeer A. , Ould Ahmed Mahmoud Sid Ahmed 
Published: August 2022

AbstractThis paper is concerned with studying a new class of multivariable commuting operators know as left $(m,C)$
(
m
,
C
)
-invertible p-tuples and related to a given conjugation transformation C on a Hilbert space $\mathcal{Y}$
Y
. Some structural properties of some members of this class are given.
Biodiesel production from a non-edible source of royna (Aphanamixis polystachya) oil
Authors: Rahman Md. Wasikur , Mondal Asim Kumar , Hasan Md. Shakil , Sultana Marzia 
Published: August 2022

Abstract
Background
Rapid consumption of fossil fuels as well as rising environmental deterioration caused by extreme CO2 emissions has become crucial in searching for a clean and renewable energy source such as biodiesel. The current work is an attempt to produce biodiesel from a potential non-edible feedstock of Aphanamixis polystachya, locally known as ‘Royna’ seed oil in Bangladesh.

Methods
Royna oil was extracted from the seed by Soxhlet extraction method. Biodiesel was synthesized by a three-step process: saponification of oil, followed by acidification of the soap, and esterification of the free fatty acid (FFA).

Results
The result presented showed that royna seed was found to be rich in oil with a maximum yield of 51% (w/w). Several reaction parameters were optimized during biodiesel production in their percentage proportion of oil to a catalyst (1:2), soap to HCl (1:1.5), FFA to an alcohol molar ratio (1:7), and catalyst (1 wt%). As a result, the highest yield of 97% was obtained from 7.5 wt% FFA content oil at 70 °C for 90-min reaction time. ASTM verified standard methods were employed to analyze the physicochemical properties of the as-prepared biodiesel. The structural and surface properties of the royna oil and as-prepared biodiesel were determined by 1H NMR and FTIR spectroscopic methods indicating a complete conversion of oil to biodiesel.

Conclusions
The study investigated the promising viability of royna oil to biodiesel using a three-step conversion route along with the heterogeneous catalysis system to circumvent the current environmental issues.