bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cationic peptide carriers enable long-term delivery of insulin-like growth factor-1 to suppress osteoarthritis-induced matrix degradation
Authors: Vedadghavami Armin , Hakim Bill , He Tengfei , Bajpayee Ambika G. 
Published: July 2022

Abstract
Background
Insulin-like growth factor-1 (IGF-1) has the potential to be used for osteoarthritis (OA) treatment but has not been evaluated in clinics yet owing to toxicity concerns. It suffers from short intra-joint residence time and a lack of cartilage targeting following its intra-articular administration. Here, we synthesize an electrically charged cationic formulation of IGF-1 by using a short-length arginine-rich, hydrophilic cationic peptide carrier (CPC) with a net charge of +14, designed for rapid and high uptake and retention in both healthy and arthritic cartilage.

Methods
IGF-1 was conjugated to CPC by using a site-specific sulfhydryl reaction via a bifunctional linker. Intra-cartilage depth of penetration and retention of CPC-IGF-1 was compared with the unmodified IGF-1. The therapeutic effectiveness of a single dose of CPC-IGF-1 was compared with free IGF-1 in an IL-1α-challenged cartilage explant culture post-traumatic OA model.

Results
CPC-IGF-1 rapidly penetrated through the full thickness of cartilage creating a drug depot owing to electrostatic interactions with negatively charged aggrecan-glycosaminoglycans (GAGs). CPC-IGF-1 remained bound within the tissue while unmodified IGF-1 cleared out. Treatment with a single dose of CPC-IGF-1 effectively suppressed IL-1α-induced GAG loss and nitrite release and rescued cell metabolism and viability throughout the 16-day culture period, while free IGF at the equivalent dose was not effective.

Conclusions
CPC-mediated depot delivery of IGF-1 protected cartilage by suppressing cytokine-induced catabolism with only a single dose. CPC is a versatile cationic motif that can be used for intra-cartilage delivery of other similar-sized drugs.
Enhanced metabolic flux of methylerythritol phosphate (MEP) pathway by overexpression of Ginkgo biloba 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate Reductase 1 (GbHDR1) gene in poplar
Authors: Kang Min-Kyoung , Kim Ji-Young , Choi Young-Im , Hu Lujie , Yang Chaodong , Jin Zhehao , Park Yun Ji , Kim Soo-Un , Kim Sang-Min 
Published: July 2022

AbstractTerpenoids are of great interests in a broad range of health-beneficial biological activities and various industrial applications. In plants, terpenoids are synthesized by two distinct pathways, methylerythritol phosphate (MEP) and mevalonate pathways in a separate location. MEP pathway supplies isoprene precursors isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP) of terpenoid biosynthesis in plant plastids. The MEP pathway has been an engineering target to increase the metabolic flux towards higher terpenoid production in plants. 1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) is the terminal step of the MEP pathway to regulate the terpenoid biosynthesis and is encoded by three paralogous genes in Ginkgo biloba. In this study, we assessed the effect of overexpression of GbHDR1 on terpenoid metabolism in poplar plants. Overexpression of GbHDR1 in poplar plants accelerated growth and delayed winter-bud formation. Transcript levels of gibberellin, chlorophylls, and carotenoid biosynthetic genes in GbHDR1-overexpressing (GbHDR1ox) poplars were up-regulated, suggesting metabolic flux enhancement. Moreover, enhanced contents of chlorophylls and carotenoids in the leaves of the GbHDR1ox plants resulted in a higher photosynthetic rate as a consequence. Therefore, we expect the GbHDR1 overexpression will be a desirable engineering point of the MEP pathway for enhancing terpenoid metabolic flux and production in plants.
Your heart is where your roots are? Place attachment and belonging among Polish and Lithuanian returnees
Authors: Trąbka Agnieszka , Klimavičiūtė Luka , Czeranowska Olga , Jonavičienė Dovile , Grabowska Izabela , Wermińska-Wiśnicka Iga 
Published: July 2022

AbstractThe reintegration of return migrants has been an important issue in migration studies for several decades. While much research has been done to identify returnees’ strategies and their labour market situation in their country of origin, little attention has been devoted to their attachment, especially in quantitative studies. This paper seeks to address this gap, analysing predictors of place attachment and belonging among Polish and Lithuanian returnees from the United Kingdom. We consider autobiographical factors connected to migration history and time spent in a place; relational factors linked to social networks, bonds and contacts; cultural factors connected to feeling “at home”; and economic and welfare factors primarily linked to economic stability and job opportunities. Our analysis is based on a web survey of Polish and Lithuanian returnees conducted in 2020 (n = 740). The results reveal that the influence of autobiographical, cultural and relational determinants on returnees’ attachment is greater than the influence of their labour market position.
Incidence, remission, and persistence of Raynaud’s phenomenon in the general population of northern Sweden: a prospective study
Authors: Stjernbrandt Albin , Pettersson Hans , Lundström Ronnie , Liljelind Ingrid , Nilsson Tohr , Wahlström Jens 
Published: July 2022

Abstract
Background
Raynaud’s phenomenon is common condition, but little is known about the natural course. The primary aim of this study was to determine the incidence, remission, and persistence proportions of Raynaud’s phenomenon in the general population of northern Sweden. Secondary aims were to determine how individual and exposure factors affect the course of Raynaud’s phenomenon, and to assess gender differences.

Methods
A prospective, survey-based, closed-cohort study was conducted on a sample of men and women between 18–70 years of age, living in northern Sweden. Data on Raynaud’s phenomenon characteristics and general health status were collected during the winters of 2015 (baseline) and 2021 (follow-up). Rates of incidence, remission, and persistence were calculated. Binary logistic regression was used to determine the association between baseline variables and the course of Raynaud’s phenomenon. Results: The study population consisted of 2703 women (53.9%) and 2314 men. There were 390 women (14.5%) and 290 men (12.7%) reporting Raynaud’s phenomenon in the follow-up survey. The annual incidence proportion was 0.7% among women and 0.9% among men (gender difference p = 0.04). The annual remission proportion was 4.4% and 5.5%, respectively (p = 0.05). Having sustained a cold injury affecting the hands since baseline was significantly associated with incident Raynaud’s phenomenon (OR 3.92; 95% CI 2.60–5.90), after adjusting for age and gender.

Conclusions
In the general population of northern Sweden, Raynaud’s phenomenon is a common but variable condition, where symptoms may remit over time. Men had a higher incidence proportion than women. The results support a possible causal pathway where cold injury can precede the onset of Raynaud’s phenomenon.
Anesthesia and cancer recurrence: an overview
Authors: Brogi Etrusca , Forfori Francesco 
Published: July 2022

AbstractSeveral perioperative factors are responsible for the dysregulation or suppression of the immune system with a possible impact on cancer cell growth and the development of new metastasis. These factors have the potential to directly suppress the immune system and activate hypothalamic-pituitary-adrenal axis and the sympathetic nervous system with a consequent further immunosuppressive effect.Anesthetics and analgesics used during the perioperative period may modulate the innate and adaptive immune system, inflammatory system, and angiogenesis, with a possible impact on cancer recurrence and long-term outcome. Even if the current data are controversial and contrasting, it is crucial to increase awareness about this topic among healthcare professionals for a future better and conscious choice of anesthetic techniques.In this article, we aimed to provide an overview regarding the relationship between anesthesia and cancer recurrence. We reviewed the effects of surgery, perioperative factors, and anesthetic agents on tumor cell survival and tumor recurrence.
Interdisciplinary Approach and Patient/Family Partners to Improve Serious Illness Conversations in Outpatient Oncology
Authors: Wasp Garrett T. , Cullinan Amelia M. , Anton Catherine P. , Williams Andy , Perry James J. , Holthoff Megan M. , Buus-Frank Madge E. 
Published: July 2022

PURPOSE: We aimed to increase Serious Illness Conversations (SIC) from a baseline of, at or near, zero to 25% of eligible patients by December 31, 2020. METHODS: We assembled an interdisciplinary team inclusive of a family partner and used the Model for Improvement as our quality improvement framework. The team developed a SMART Aim, key driver diagram, and SIC workflow. Standardized screening for SIC eligibility was implemented using the 2-year surprise question. Team members were trained in SIC communication skills by a trained facilitator and received ongoing coaching in quality improvement. We performed Plan-Do-Study-Act cycles and used audit-feedback data in weekly team meetings to inform iterative Plan-Do-Study-Act cycles. The primary outcome was the percent of eligible patients with documented SIC. RESULTS: Over 18 months, three clinics identified 63 eligible patients; of these, 32 (51%) were diagnosed with head and neck cancer and 31 (49%) with sarcoma. The SIC increased from a baseline near zero to 43 of 63 (70%) patients demonstrating three shifts in the median (95% CI). Conversations were interdisciplinary with 25 (57%) by oncology MD, six (14%) by advanced practice registered nurse, and 13 (30%) by specialty palliative care. We targeted four key drivers: (1) standardized work, (2) engaged interdisciplinary team, (3) engaged patients and families, and (4) system-level support. CONCLUSION: Our approach was successful in its documentation of end points and required resource investment (training and time) to embed into team workflows. Future work will evaluate scaling the approach across multiple clinics, the patient experience, and outcomes of care associated with oncology clinician–led SIC.
Face-sparing Congenital Generalized Lipodystrophy Type 1 Associated With Nonclassical Congenital Adrenal Hyperplasia
Authors: Costa Sara , Sampaio Lurdes , Berta Sousa Ana , Xing Chao , Agarwal Anil K , Garg Abhimanyu 
Published: July 2022

Abstract

Context
Congenital generalized lipodystrophy, type 1 (CGL1), due to biallelic pathogenic variants in AGPAT2, is characterized by the near total loss of body fat from the face, trunk, and extremities. Patients develop premature diabetes, hypertriglyceridemia, hepatic steatosis, and polycystic ovary syndrome. However, sparing of the facial fat and precocious pubertal development has not been previously reported in CGL1.

Case Description
We report a 21-year-old woman of European descent with CGL1 who had sparing of the facial fat and premature thelarche at birth with premature pubarche and menstrual bleeding at age 3 years. Her serum 17-OH progesterone level rose to 1000 ng/dL (30.26 nmol/L) after cosyntropin stimulation test, suggestive of nonclassical congenital adrenal hyperplasia (NCAH) due to 21-hydroxylase deficiency. Hydrocortisone replacement therapy from age 3.5 to 10 years resulted in cessation of menstruation and growth of pubic hair, and a reduction of breast size. Sanger and whole-exome sequencing revealed compound heterozygous variants c.493-1G&gt;C; p.(Leu165_Gln196del), and c.del366_588+534; p.(Leu123Cysfs*55) in AGPAT2 plus c.806G&gt;C; p.(Ser269Thr) and c.844G&gt;T; p.(Val282Leu) in CYP21A2. She developed diabetes at age 13 requiring high-dose insulin and had 7 episodes of acute pancreatitis due to extreme hypertriglyceridemia in the next 5 years. Metreleptin therapy was initiated at age 18 and after 3 years, she had remission of diabetes and hypertriglyceridemia; however, menstrual irregularity and severe hirsutism did not improve.

Conclusion
Concomitant NCAH in this CGL1 patient was associated with precocious pubertal development and sparing of facial fat. Metreleptin therapy drastically improved her hyperglycemia and hyperlipidemia but not menstrual irregularity and hirsutism.
Poetry, Ghosts, Mediation
Authors: Chen Jack W. 
Published: July 2022

AbstractThis essay takes the example of a poem composed by a ghost in the Tang dynasty—one of many preserved in literary anthologies and treated as actually having been authored by the dead—as an entry point to ask broader questions of ghostly haunting and poetic presence. What the essay demonstrates is how both the ghost and the poem are informed by logics of analog mediation (rather than representation): how the ghost finds purchase in the world only through bodily possession, spatial haunting, material displacement, and psychic transference and how the poem effects the transmission of mind through the channels of linguistic form, meter, and rhyme. Neither the ghost nor the poem exists except in or as its mediations, yet through these mediations, both the ghost and the poem become present and are communicated into the world. While contemporary media theory has identified the intertwined discourses of technology and spiritualism, the focus has almost solely been on the nineteenth century and later, on the age of electric and electronic telecommunications. The medieval ghost poem, as an exemplary case, complicates this account, showing how poetry has long served as a necrotechnology that mediates the dead and returns ghosts to presence.
The Para-Worlds of Lesley Nneka Arimah’sWhat It Means When a Man Falls from the Sky
Authors: Kumavie Delali 
Published: July 2022

AbstractThis essay argues that Lesley Nneka Arimah’s collection of short stories, What It Means When a Man Falls from the Sky, evokes para-worlds that reveal and contend with the world and its norms. Examining the collection’s entwinement of magical, mythical, and animist modes with science, technology, and innovation, the essay shows how Arimah’s work creates its own metagenre, which functions as a paranormal frame that disrupts the common sense of existing interpretive frameworks of progress and difference. The paranormal in Arimah’s work is a space where the illegible significance of Blackness exists. These para-worlds, the essay contends, are paranormal because they function as parallel, ancillary, and barricading structures that reveal the spatial and temporal continuums that order the world. Viewing the norm as the world-constituting structures instantiated in 1492, the essay contends that Arimah’s para-world functions as a continuum to reveal the ever-present structures of violence.
Wood ants learn the magnetic direction of a route but express uncertainty because of competing directional cues
Authors: Collett Thomas S. , Philippides Andrew O. 
Published: July 2022

ABSTRACT
Wood ants were trained indoors to follow a magnetically specified route that went from the centre of an arena to a drop of sucrose at the edge. The arena, placed in a white cylinder, was in the centre of a 3D coil system generating an inclined Earth-strength magnetic field in any horizontal direction. The specified direction was rotated between each trial. The ants’ knowledge of the route was tested in trials without food. Tests given early in the day, before any training, show that ants remember the magnetic route direction overnight. During the first 2 s of a test, ants mostly faced in the specified direction, but thereafter were often misdirected, with a tendency to face briefly in the opposite direction. Uncertainty about the correct path to take may stem in part from competing directional cues linked to the room. In addition to facing along the route, there is evidence that ants develop magnetically directed home and food vectors dependent upon path integration. A second experiment asked whether ants can use magnetic information contextually. In contrast to honeybees given a similar task, ants failed this test. Overall, we conclude that magnetic directional cues can be sufficient for route learning.
Free Energy and Solvation Structure Analysis for Adsorption of Aromatic Molecules at Pt(111)/Water Interface by 3D-RISM Theory
Authors: Takamatsu Akihiko , Higashi Masahiro , Sato Hirofumi 
Published: July 2022

Abstract
The free energy change of aromatic molecules adsorbed at a Pt(111)/water interface was analyzed using the three-dimensional reference interaction site model (3D-RISM) theory with density functional theory (DFT), compared with the reported experimental data. The changes in the solvation structure induced by molecular adsorption were discussed.
DSMZCellDive: Diving into high-throughput cell line data
Authors: Koblitz Julia , Dirks Wilhelm G. , Eberth Sonja , Nagel Stefan , Steenpass Laura , Pommerenke Claudia 
Published: July 2022

Human and animal cell lines serve as model systems in a wide range of life sciences such as cancer and infection research or drug screening. Reproducible data are highly dependent on authenticated, contaminant-free cell lines, no better delivered than by the official and certified biorepositories. Offering a web portal to high-throughput information on these model systems will facilitate working with and comparing to these references by data otherwise dispersed at different sources. We here provide DSMZCellDive to access a comprehensive data source on human and animal cell lines, freely available at celldive.dsmz.de. A wide variety of data sources are generated such as RNA-seq transcriptome data and STR (short tandem repeats) profiles. Several starting points ease entering the database via browsing, searching or visualising. This web tool is designed for further expansion on meta and high-throughput data to be generated in future. Explicated examples for the power of this novel tool include analysis of B-cell differentiation markers, homeo-oncogene expression, and measurement of genomic loss of heterozygosities by an enlarged STR panel of 17 loci. Sharing the data on cell lines by the biorepository itself will be of benefit to the scientific community since it (1) supports the selection of appropriate model cell lines, (2) ensures reliability, (3) avoids misleading data, (4) saves on additional experimentals, and (5) serves as reference for genomic and gene expression data.
Impact of Motivation and Workload on Service Time Components: An Empirical Analysis of Call Center Operations
Authors: Ashkanani Ahmad M. , Dunford Benjamin B. , Mumford Kevin J. 
Published: July 2022

We study the joint effects of motivation and workload on human servers’ service time. Using operational and survey data from a call center with a pooled queue structure and limited financial incentives, we examine how individual differences between servers’ trait intrinsic motivation (IM) and extrinsic motivation (EM) impact their average offline, online, and total service times in response to changing workloads. We find significant differences in the patterns of workload and service time relationships across different stages of the service request between servers possessing different combinations of trait motivation. For example, servers with a combination of high IM and low EM were approximately 15% (161%) faster in processing the offline portion of service requests than their peers with the opposite combination (low and high) when workload levels were low (high), respectively. In contrast, servers with high IM-low EM were approximately 35% (5%) slower in processing the online portion of service requests than their low IM-high EM counterparts when workload levels were low (high), respectively. Our findings suggest important nuances in how servers with different trait motivation types respond to changing workload across different stages of the service request. The behavioral pattern shown by high IM-low EM servers is consistent with the preferences of productivity-seeking call center managers who favor speedup and slowdown at certain stages of the service request, conditional to workload. These findings underscore the importance of accounting for trait-based individual differences for a more complete understanding of the complex relationship between workload and service time. This paper was accepted by Charles Corbett, operations management.
Vascular access teams: a global outlook on challenges, benefits, opportunities, and future perspectives
Authors: Morrow Shonda , DeBoer Erica , Potter Christopher , Gala Smeet , Alsbrooks Kimberly 
Published: July 2022

Background: Specialized vascular access training for medical professionals organized into vascular access teams (VATs) was shown to improve patient outcomes, clinical efficiency, and cost savings. Professional perspectives on VAT benefits, organization, challenges, and opportunities on a global scale remain inadequately explored. Using detailed perspectives, in this study, we explored the global VAT landscape, including challenges faced, clinical and clinico-economic impacts of VATs, with emphasis on underresearched facets of VAT initiation, data dissemination, and metrics or benchmarks for VAT success. Methods: Semistructured in-depth interviews of 14 VAT professionals from 9 countries and 5 continents were used to elicit qualitative and quantitative information. Results: Catheter insertions (100%) and training (86%) were the most performed VAT functions. Based on a 1–7 scale evaluating observed impacts of VATs, patient satisfaction (6.5) and institutional costs (6.2) were ranked the highest. VAT co-initiatives, advanced technology utilization (6.6), and ongoing member training (6.3) distinctly impacted VAT endeavors. Most institutions (64%) did not have routine mechanisms for recording VAT-related data; however, all participants (100%) stated the importance of sharing data to demonstrate VAT impacts. Time constraints (57%) emerged as one of the major deterrents to data collection or dissemination. The majority (64%) experienced an increased demand or workload for VAT services during the COVID-19 pandemic. Conclusions: Despite the global variances in VATs and gaps in VAT-related data, all participants unanimously endorsed the benefits of VAT programs. Evaluating the impact of VATs, disseminating VAT-related data, and forging specialized institutional partnerships for data sharing and training are potential strategies to tackle the hurdles surrounding VAT formation and sustenance.