bluuebunny/crossref_metadata_2025_split huggingface dataset:
Date labels, food waste and supply chain implications
Authors: Rickard Bradley J , Ho Shuay-Tsyr , Livat Florine , Okrent Abigail M 
Published: September 2022

Abstract
We developed a survey to collect information on consumers’ intentions to discard 15 food products when exposed to different date labels. Results show that the use of certain date labels has the capacity to reduce food waste, but the reductions would happen differentially across food groups. When we examine the nutritional implications, we find that a shift from the ‘Best by’ date label to the ‘Best if Used by’ date label would increase total household purchases of energy and lead to a disproportional increase in purchases of fats, cholesterol and protein.
Poetry and the Language of Otherness: Baudelaire, Mallarmé, Ponge
Authors: Poiana Peter 
Published: September 2022

Abstract
The language of otherness is a discursive framework that applies to modern poetry in the way it responds to the disappearance of stable forms of legitimization. This study focuses on the writings of three poets, Baudelaire, Mallarmé and Ponge, all of whom have approached in a vexed manner the task of poetic innovation because they were all preoccupied to some degree by the question of authentication. It analyses selected writings of the poets by highlighting the affective polarities that emerge in their works, particularly through the intellectual trials to which they subject themselves, but also in their assessment of their place in a literary tradition that, as modern poets, they seek to overturn. The analysis is based on Lacan’s notion of the big Other as the site of authorization but also of the misestimation of desire inasmuch as it conveyed in language.
Cell-trafficking impairment in disease-associated LPA6 missense mutants and a potential pharmacoperone therapy for autosomal recessive woolly hair/hypotrichosis
Authors: Yanagida Keisuke , Masago Kayo , Yasuda Daisuke , Hamano Fumie , Kurikawa Yoshitaka , Shimizu Takao , Ishii Satoshi 
Published: September 2022

AbstractIn human autosomal recessive woolly hair/hypotrichosis (ARWH/HT), many mutations have been identified in a gene encoding LPA6, a G protein-coupled receptor (GPCR) for lysophosphatidic acid (LPA). However, information regarding the effects of such mutations on receptor function is limited. In this study, we examined functional impacts of selected amino acid changes in LPA6 identified in ARWH/HT patients. In our exogenous expression experiments, all mutants except S3T failed to respond to LPA, indicating that they are loss-of-function mutants. Among the nine mutants, five (D63V, G146R, N246D, L277P and C278Y) displayed impaired expression at the cell surface because of endoplasmic reticulum (ER) retention, indicating that these mutants are trafficking-defective, as reported in other disease-associated GPCRs. Notably, alkyl-OMPT, a potent synthetic agonist for LPA6 restored the defective cell surface expression of two of the ER-retained mutants, D63V and N246D, possibly by its chaperoning function that allows them to escape intracellular retention as well as proteasomal degradation. Furthermore, the alkyl-OMPT-rescued N246D mutant was shown be functional. Our findings encourage future application of pharmacoperone therapy for ARWH/HT patients with specific LPA6 mutations.
Against settlement before the European Court of Human Rights
Authors: Fikfak Veronika 
Published: September 2022

Abstract
Even though they represent almost 50% of all reported cases before the European Court of Human Rights (ECtHR), settlements of human rights violations escape scholars’ attention. While victims are increasingly expected to resolve their disputes amicably, it is unclear whether applicants will be better off accepting settlement offers rather than proceeding to litigation. The article charts the practice of friendly settlements before the Court from the 1980s to today, mapping a shift in approach from seeking bilateral solutions to the proactive role of the Registry as mediator encouraging states and applicants to settle their cases to relieve the Court of the heavy workload. The study of 10,500 cases reveals how strategies adopted by the Registry—from procedural changes to how and when consent is given to settlement, to the framing of settlement offers, and a close relationship with representatives of the respondent state—have favored the most frequent violators of the European Convention on Human Rights and sidelined the interests of the applicant. The analysis uncovers that the imbalance between parties and lack of enforcement are very much present in the ECtHR settlement system and that the active role of the Registry has reinforced, rather than redressed these concerns. The findings expose the dangers of pursuing en masse settlement in the human rights context and raise concerns about achieving long-term justice for victims of human rights violations through other means than adjudication.
Impact of Brazil’s Bolsa Família Programme on cardiovascular and all-cause mortality: a natural experiment study using the 100 Million Brazilian Cohort
Authors: Pescarini Julia M , Campbell Desmond , Amorim Leila D , Falcão Ila R , Ferreira Andrêa J F , Allik Mirjam , Shaw Richard J , Malta Deborah C , Ali M Sanni , Smeeth Liam , Barreto Mauricio L , Leyland Alastair , Craig Peter , Aquino Estela M L , Katikireddi Srinivasa Vittal 
Published: September 2022

Abstract

Background
Cardiovascular disease (CVD) has a disproportionate effect on mortality among the poorest people. We assessed the impact on CVD and all-cause mortality of the world's largest conditional cash transfer, Brazil’s Bolsa Família Programme (BFP).

Methods
We linked administrative data from the 100 Million Brazilian Cohort with BFP receipt and national mortality data. We followed individuals who applied for BFP between 1 January 2011 and 31 December 2015, until 31 December 2015. We used marginal structural models to estimate the effect of BFP on all-age and premature (30–69 years) CVD and all-cause mortality. We conducted stratified analyses by levels of material deprivation and access to healthcare. We checked the robustness of our findings by restricting the analysis to municipalities with better mortality data and by using alternative statistical methods.

Results
We studied 17 981 582 individuals, of whom 4 855 324 were aged 30–69 years. Three-quarters (76.2%) received BFP, with a mean follow-up post-award of 2.6 years. We detected 106 807 deaths by all causes, of which 60 893 were premature; and 23 389 CVD deaths, of which 15 292 were premature. BFP was associated with reductions in premature all-cause mortality [hazard ratio (HR) = 0.96, 95% CI = 0.94–0.98], premature CVD (HR = 0.96, 95% CI = 0.92–1.00) and all-age CVD (HR = 0.96, 95% CI = 0.93–1.00) but not all-age all-cause mortality (HR = 1.00, 95% CI = 0.98–1.02). In stratified and robustness analyses, BFP was consistently associated with mortality reductions for individuals living in the two most deprived quintiles.

Conclusions
BFP appears to have a small to null effect on premature CVD and all-cause mortality in the short term; the long-term impact remains unknown.
Nonlinear dynamic responses of an inclined beam to harmonic excitation in temperature field
Authors: Zhou Liangqiang , Chen Fangqi 
Published: September 2022

Abstract
Using both analytical and numerical methods, nonlinear dynamic behaviours including chaotic motions and subharmonic bifurcations of an inclined beam subjected to harmonic excitation in temperature field are investigated in this paper. Based on the Galerkin method, the mathematical model of motion is derived. Melnikov method is adopted to give an analytical expression of conditions for chaotic motions of the inclined beam. The chaotic feature on the inclined angle is studied in detail. It is presented that there exists a unique excitation frequency $\omega ^*$, such that the critical value of chaos is the monotone decreasing function of the inclination angle when the excitation frequency $\omega &lt;\omega ^*$; whereas $\omega&gt;\omega ^*$, it is the monotone increasing function of the inclination angle. The subharmonic bifurcations are also studied. It is obtained that subharmonic bifurcations of even orders or odd orders may occur for this system. With the techniques of elliptic functions, it is proved rigorously that this system may undergo chaos through finite subharmonic bifurcations. Numerical simulations are given to verify the chaos threshold obtained by the analytical method.
Activation of Laminin γ2 byHelicobacter pyloriPromotes Invasion and Survival of Gastric Cancer Cells With E-Cadherin Defects
Authors: Ferreira Rui M , Figueiredo Joana , Pinto-Ribeiro Ines , Gullo Irene , Sgouras Dionyssios N , Carreto Laura , Castro Patricia , Santos Manuel A , Carneiro Fatima , Seruca Raquel , Figueiredo Ceu 
Published: September 2022

AbstractBackgroundHelicobacter pylori infection induces cellular phenotypes relevant for cancer progression, namely cell motility and invasion. We hypothesized that the extracellular matrix (ECM) could be involved in these deleterious effects.MethodsMicroarrays were used to uncover ECM interactors in cells infected with H. pylori. LAMC2, encoding laminin γ2, was selected as a candidate gene and its expression was assessed in vitro and in vivo. The role of LAMC2 was investigated by small interference RNA (siRNA) combined with a set of functional assays. Laminin γ2 and E-cadherin expression patterns were evaluated in gastric cancer cases.ResultsLaminin γ2 was found significantly overexpressed in gastric cancer cells infected with H. pylori. This finding was validated in vitro by infection with clinical isolates and in vivo by using gastric biopsies of infected and noninfected individuals. We showed that laminin γ2 overexpression is dependent on the bacterial type IV secretion system and on the CagA. Functionally, laminin γ2 promotes cell invasion and resistance to apoptosis, through modulation of Src, JNK, and AKT activity. These effects were abrogated in cells with functional E-cadherin.ConclusionsThese data highlight laminin γ2 and its downstream effectors as potential therapeutic targets, and the value of H. pylori eradication to delay gastric cancer onset and progression.
A designed cyclic analogue of gomesin has potent activity against Staphylococcus aureus biofilms
Authors: Dias Susana A , Pinto Sandra N , Silva-Herdade Ana S , Cheneval Olivier , Craik David J , Coutinho Ana , Castanho Miguel A R B , Henriques Sónia T , Veiga Ana Salomé 
Published: September 2022

Abstract

Background
Infections caused by bacterial biofilms are very difficult to treat. The use of currently approved antibiotics even at high dosages often fails, making the treatment of these infections very challenging. Novel antimicrobial agents that use distinct mechanisms of action are urgently needed.

Objectives
To explore the use of [G1K,K8R]cGm, a designed cyclic analogue of the antimicrobial peptide gomesin, as an alternative approach to treat biofilm infections.

Methods
We studied the activity of [G1K,K8R]cGm against biofilms of Staphylococcus aureus, a pathogen associated with several biofilm-related infections. A combination of atomic force and real-time confocal laser scanning microscopies was used to study the mechanism of action of the peptide.

Results
The peptide demonstrated potent activity against 24 h-preformed biofilms through a concentration-dependent ability to kill biofilm-embedded cells. Mechanistic studies showed that [G1K,K8R]cGm causes morphological changes on bacterial cells and permeabilizes their membranes across the biofilm with a half-time of 65 min. We also tested an analogue of [G1K,K8R]cGm without disulphide bonds, and a linear unfolded analogue, and found both to be inactive.

Conclusions
The results suggest that the 3D structure of [G1K,K8R]cGm and its stabilization by disulphide bonds are essential for its antibacterial and antibiofilm activities. Moreover, our findings support the potential application of this stable cyclic antimicrobial peptide to fight bacterial biofilms.
Niclosamide as a repurposing drug against Gram-positive bacterial infections
Authors: Zhang Wei , Ran Jinxin , Shang Lu , Zhang Lifang , Wang Mi , Fei Chenzhong , Chen Chan , Gu Feng , Liu Yingchun 
Published: September 2022

Abstract

Objectives
Niclosamide is commonly used as an antiparasitic drug in veterinary clinics. The objectives of this study were to evaluate the efficacy of niclosamide against resistant Gram-positive bacteria in vitro and in an in vivo experimental model of topical bacterial infection. Moreover, to study the antibacterial mechanism of niclosamide to Staphylococcus aureus.

Methods
A mouse topical infection model was established to detect the antibacterial activity of niclosamide in vivo. The antimicrobial mechanism was probed by visualizing the bacterial morphologies using scanning electron microscopy and transmission electron microscopy. Moreover, the haemolytic assay and western blotting analysis were performed to evaluate whether niclosamide could inhibit the secretion of alpha-haemolysin (α-HL) from S. aureus.

Results
The MICs of niclosamide were below 0.5 mg/L for Gram-positive bacteria, showing excellent antibacterial activity in vitro. The in vivo antibacterial activity results indicated that niclosamide treatment at 10 mg/kg of body weight caused a significant reduction in the abscess area and the number of S. aureus cells. Moreover, the antibacterial mechanism of niclosamide showed that the surface morphology of S. aureus displayed noticeable shrinkage, with an increasing number of small vacuole-like structures observed as the drug concentration increased. Intracellular ATP levels were found to decrease in a niclosamide dose-dependent manner. Haemolysis and western blotting analyses revealed that niclosamide inhibited the haemolytic activity of S. aureus by inhibiting α-HL expression under subinhibitory concentration conditions.

Conclusions
Niclosamide has significant potential for development into drugs that prevent and treat diseases caused by Gram-positive bacteria such as Staphylococcus and Streptococcus.
Cefepime pharmacodynamic targets against Enterobacterales employing neutropenic murine lung infection and in vitro pharmacokinetic models
Authors: Muller Anouk E , Attwood Marie , Van den berg Sanne , Chavan Rajesh , Periasamy Hariharan , Noel Alan , MacGowan Alasdair 
Published: September 2022

Abstract

Background
Very limited studies, so far, have been conducted to identify the pharmacodynamic targets of cefepime, a well-established fourth-generation cephalosporin. As a result, conventional targets representing the cephalosporin class are used for cefepime target attainment analysis.

Objectives
We employed both a neutropenic murine lung infection model and an in vitro pharmacokinetic model (IVPM) to determine cefepime’s pharmacodynamic target [percentage of the dosing interval during which unbound drug concentrations remain higher than the MIC (%fT&gt;MIC)] for bacteriostatic and 1 log10 kill effects.

Methods
Ten strains with cefepime MICs ranging from 0.03 to 16 mg/L were studied in the lung infection. In the IVPM, five cefepime-resistant strains with cefepime/tazobactam (fixed 8 mg/L) MICs ranging from 0.25 to 8 mg/L were included. Through 24 h dose fractionation, both in lung infection and IVPM (in the latter case, tazobactam 8 mg/L continuous infusion was used to protect cefepime), varying cefepime exposures and corresponding pharmacodynamic effect scenarios were generated to identify the pharmacodynamic targets.

Results
Using a non-linear sigmoidal maximum-effect (Emax) model, the cefepime’s plasma fT&gt;MIC for 1 log10 kill in lung infection ranged from 17% to 53.7% and a combined exposure–response plot yielded 30%. In the case of IVPM, T&gt;MIC ranged from 6.9% to 75.4% with a mean value of 34.2% for 1 log10 kill.

Conclusions
Both in vivo and in vitro studies showed that cefepime’s pharmacodynamic requirements are lower than generally reported for cephalosporins (50%–70% fT&gt;MIC). The lower requirement for cefepime could be linked with factors such as cefepime’s better permeation properties and multiple PBP affinity-driven enhanced bactericidal action.
Pediatric Hematopoietic Stem Cell Transplant Medication Adherence Facilitators and Strategies: A Qualitative Study
Authors: Chardon Marie L , Klages Kimberly L , Joffe Naomi E , Pai Ahna L H 
Published: September 2022

Abstract

Objective
Taking medications as prescribed after hematopoietic stem cell transplant (HCT) is key for ensuring children’s survival; however, suboptimal medication adherence is common. Development of evidence-based interventions to improve medication adherence post-HCT is contingent upon understanding what adherence facilitators (i.e., unique traits, characteristics, or resources inherent to the individual, medical treatment, or healthcare team) and strategies (i.e., tools caregivers or medical providers intentionally use) promote medication adherence in this population. Therefore, this study examined caregiver-perceived medication facilitators post-HCT.

Methods
Semi-structured qualitative interviews and demographic questionnaires were completed by 29 caregivers of children (≤12 years) who had received an HCT within the past 2 years.

Results
Thematic analysis guided by grounded theory revealed 14 saturated themes that were grouped into 4 categories: family facilitators, medication facilitators, caregiver strategies, and multidisciplinary treatment team strategies.

Conclusions
Overall, findings suggest that caregivers of children who received an HCT are highly resourceful and independently develop many strategies to assist them with medication management after their child’s HCT. These facilitators and strategies varied between caregivers and over time. Despite prevalent facilitators and strategies, caregiver burden associated with medication adherence remains high. Caregivers may benefit from the multidisciplinary treatment team providing individualized and multicomponent (educational and behavioral) medication adherence supports to ease this burden particularly shortly after hospital discharge.
Colonic lipoma of the hepatic flexure causing invagination in an adult
Authors: Palacios Mauricio Fabian , Guachilema Ribadeneira Alex , Morocho Imbacuan Sandra , Lisintuña Cisneros Andrea , Basantes Andrea Daniela 
Published: September 2022

Abstract
Intussusception is a rare condition in adults that can lead to intestinal obstruction due to malignant or benign lesions in the wall of the colon. Among the benign causes, lipomas are adipose tissue tumors that are most frequently located in the ascending colon. Invagination is characterized by nonspecific symptoms and normal laboratory test results. The exact point of invagination can be detected using advanced imaging studies such as abdominal computed tomography and colonoscopy. Invagination is treated surgically. Here, we presented the case of a female patient with colo-colonic invagination caused by a large lipoma diagnosed by computed tomography who underwent right hemicolectomy with primary ileocolonic anastomosis.
A rare case of oesophageal and gastric fundus necrosis following traumatic splenectomy
Authors: Bennett Laura , Gallagher James 
Published: September 2022

Abstract
Oesophageal and gastric fundus necrosis are rare clinical entities and have not previously been reported concurrently. This case study describes oesophageal and gastric fundus necrosis in a 71-year-old female who developed chest pain post-splenectomy for infected splenic haematoma in the context of a fall. Upper gastrointestinal endoscopy demonstrated oesophageal and gastric fundus necrosis. This was likely due to multiple factors including comorbidities, hypoperfusion, post-splenectomy state and herpes simplex virus oesophagitis. This finding is an important differential in the unwell patient with chest pain post-splenectomy and investigation with upper gastrointestinal endoscopy could be considered in the absence of another cause.
AKG/OXGR1 promotes skeletal muscle blood flow and metabolism by relaxing vascular smooth muscle
Authors: Yang Jinping , Xu Guli , Xu Yiming , Luo Pei , Yuan Yexian , Yao Lin , Zhou Jingjing , Zhu Yunlong , Gyawali Ishwari , Xu Chang , Feng Jinlong , Ma Zewei , Zeng Yuxian , Wang Songbo , Gao Ping , Zhu Canjun , Jiang Qingyan , Shu Gang 
Published: September 2022

Abstract
In response to contraction during exercise, skeletal muscle growth and metabolism are dynamically regulated by nerve action, blood flow, and metabolic feedback. α-Ketoglutarate (AKG), a bioactive intermediate in the tricarboxylic acid cycle released during exercise, has been shown to promote skeletal muscle hypertrophy. However, the underlying mechanism of AKG in regulating skeletal muscle development and metabolism is still less known. 2-Oxoglutarate receptor 1 (OXGR1), the endogenous AKG receptor, is found to be distributed in the vascular smooth muscle (VSM) of skeletal muscles. OXGR1 knockout results in skeletal muscle atrophy, accompanied by decreased expression of myosin heavy chain I (MyHC I), capillary density, and endurance exercise capacity. Furthermore, the study found that dietary AKG supplementation increased mice endurance exercise distance, MyHC I/MyHC IIb ratio, arteriole, and capillary densities in skeletal muscle. Meanwhile, acute AKG administration gradually increased the blood flow in the lower limbs. Further, by using OXGR1 global knockout and OXGR1 VSM-specific (MYH11-Cre × OXGR1-FloxP) knockdown models, we found that OXGR1 in VSM is essential for AKG-induced improvement of skeletal muscle performances. According to the in vitro study, AKG expanded the cell area in VSM with a decreased intracellular pH by OXGR1. Our results demonstrated a novel role of AKG/OXGR1 in VSM of skeletal muscle to regulate blood flow and then enhance slow muscle fiber conversion and capillarization. These findings provide a theoretical basis for the AKG/OXGR1 signaling pathway to maintain human muscle function and improve meat production and livestock and poultry meat quality.
Machine learning-based search for cataclysmic variables within Gaia Science Alerts
Authors: Mistry D , Copperwheat C M , Darnley M J , Olier I 
Published: September 2022

ABSTRACT
Wide-field time domain facilities detect transient events in large numbers through difference imaging. For example, Zwicky Transient Facility produces alerts for hundreds of thousands of transient events per night, a rate set to be dwarfed by the upcoming Vera C. Rubin Observatory. The automation provided by machine learning (ML) is therefore necessary to classify these events and select the most interesting sources for follow-up observations. Cataclysmic variables (CVs) are a transient class that are numerous, bright, and nearby, providing excellent laboratories for the study of accretion and binary evolution. Here we focus on our use of ML to identify CVs from photometric data of transient sources published by the Gaia Science Alerts (GSA) program – a large, easily accessible resource, not fully explored with ML. Use of light-curve feature extraction techniques and source metadata from the Gaia survey resulted in a random forest model capable of distinguishing CVs from supernovae, active galactic nuclei, and young stellar objects with a 92 per cent precision score and an 85 per cent hit rate. Of 13 280 sources within GSA without an assigned transient classification our model predicts the CV class for ∼2800. Spectroscopic observations are underway to classify a statistically significant sample of these targets to validate the performance of the model. This work puts us on a path towards the classification of rare CV subtypes from future wide-field surveys such as the Legacy Survey of Space and Time.