bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional characterization and transcriptional repression by Lacticaseibacillus paracasei DinJ-YafQ
Authors: Bonini Aleksandra Anna , Maggi Stefano , Mori Giulia , Carnuccio Dario , Delfino Danila , Cavazzini Davide , Ferrari Alberto , Levante Alessia , Yamaguchi Yoshihiro , Rivetti Claudio , Folli Claudia 
Published: October 2022

Abstract
DinJ-YafQ is a bacterial type II TA system formed by the toxin RNase YafQ and the antitoxin protein DinJ. The activity of YafQ and DinJ has been rigorously studied in Escherichia coli, but little has been reported about orthologous systems identified in different microorganisms. In this work, we report an in vitro and in vivo functional characterization of YafQ and DinJ identified in two different strains of Lacticaseibacillus paracasei and isolated as recombinant proteins. While DinJ is identical in both strains, the two YafQ orthologs differ only for the D72G substitution in the catalytic site. Both YafQ orthologs digest ribosomal RNA, albeit with different catalytic efficiencies, and their RNase activity is neutralized by DinJ. We further show that DinJ alone or in complex with YafQ can bind cooperatively to a 28-nt inverted repeat overlapping the −35 element of the TA operon promoter. Atomic force microscopy imaging of DinJ-YafQ in complex with DNA harboring the cognate site reveals the formation of different oligomeric states that prevent the binding of RNA polymerase to the promoter. A single amino acid substitution (R13A) within the RHH DNA-binding motif of DinJ is sufficient to abolish DinJ and DinJ-YafQ DNA binding in vitro. In vivo experiments confirm the negative regulation of the TA promoter by DinJ and DinJ-YafQ and unveil an unexpected high expression-related toxicity of the gfp reporter gene. A model for the binding of two YafQ-(DinJ)2-YafQ tetramers to the promoter inverted repeat showing the absence of protein-protein steric clash is also presented.

Key points
• The RNase activity of L. paracasei YafQ toxin is neutralized by DinJ antitoxin.
• DinJ and DinJ-YafQ bind to an inverted repeat to repress their own promoter.
• The R13A mutation of DinJ abolishes DNA binding of both DinJ and DinJ-YafQ.
The potential of rectal swabs to differentiate simple and complex appendicitis in children with a microbiota-based test
Authors: The Sarah-May M. L. , de Meij Tim G. J. , Budding Andries E. , Bakx Roel , van der Lee Johanna H. , Poort Linda , Cense Huib A. , Heij Hugo A. , van Heurn L. W. Ernst , Gorter Ramon R. 
Published: October 2022

AbstractCurrently, accurate biomarkers differentiating simple (phlegmonous) from complex (gangrenous and/or perforated) appendicitis in children are lacking. However, both types may potentially require different treatment strategies, and the search for diagnostic modalities remains warranted. Previously, we demonstrated a distinct microbiota (both an increased bacterial diversity and abundance) in the appendix of children with complex compared to simple appendicitis. From the same cohort of patients we have collected 35 rectal swabs under general anesthesia prior to appendectomy and microbiota analysis was performed by IS-pro, a 16S-23S rDNA–based clinical microbiota profiling technique. Using the obtained IS-profiles, we performed cluster analyses (UPGMA), comparison of diversity (Shannon Diversity Index) and intensity (abundance in relative fluorescence units) on phylum level, and comparison on species level of bacteria between simple and complex appendicitis. Regarding these analyses, we observed no clear differences between simple and complex appendicitis. However, increased similarity of the microbial composition of the appendix and rectal swab was found within children with complex compared to simple appendicitis. Furthermore, PLS-DA regression analysis provided clear visual differentiation between simple and complex appendicitis, but the diagnostic power was low (highest AUC 0.65). Conclusion: Microbiota analysis of rectal swabs may be viable to differentiate between simple and complex appendicitis prior to surgery as a supervised classification model allowed for discrimination of both types. However, the current diagnostic power was low and further validation studies are needed to assess the value of this method.

What is Known:• Simple and
complex appendicitis in children may require different treatment strategies,
but accurate preoperative biomarkers are lacking.• Clear differentiation
can be made between both types in children based upon the microbial composition
in the appendix.

What is New:• Increased similarity
was found between the microbial composition of the appendix and rectal swab
within children with complex compared to simple appendicitis.• Using
a supervised classification model rectal swabs may be viable to discriminate
between simple and complex appendicitis, but the diagnostic power was low.
A Lower Bound on the Number of Generators of a Defect Group
Authors: Rodríguez Carolina Vallejo 
Published: October 2022

AbstractAssuming that the statement of the Alperin–McKay–Navarro conjecture holds for a p-block B with defect group D, we show that the number of generators of D is bounded from below by the number of height-zero characters in B fixed by a specific element of the absolute Galois group of the rational numbers.
The Effects of a Perindopril-Based Regimen in Relation to Statin Use on the Outcomes of Patients with Vascular Disease: a Combined Analysis of the ADVANCE, EUROPA, and PROGRESS Trials
Authors: Radhoe S. P. , Boersma E. , Bertrand M. , Remme W. , Ferrari R. , Fox K. , MacMahon S. , Chalmers J. , Simoons M. L. , Brugts J. J. 
Published: October 2022

Abstract

Purpose
To study the effects of a perindopril-based regimen on cardiovascular (CV) outcomes in patients with vascular disease in relation to background statin therapy.

Methods
A pooled analysis of the randomized ADVANCE, EUROPA, and PROGRESS trials was performed to evaluate CV outcomes in 29,463 patients with vascular disease treated with perindopril-based regimens versus placebo. The primary endpoint was a composite of CV mortality, nonfatal myocardial infarction, and stroke. Multivariable Cox regression analyses were performed to assess the effects of a perindopril-based regimen versus placebo in relation to statin use.

Results
At randomization, 39.5% of the overall combined study population used statins. After a mean follow-up of 4.0 years (SD 1.0), the cumulative event-free survival was highest in the statin/perindopril group and lowest in the no statin/placebo group (91.2% vs. 85.6%, respectively, log-rank p < 0.001). In statin users (adjusted hazard ratio [aHR] 0.87, 95% confidence interval [CI] 0.77–0.98) and non-statin users (aHR 0.80, 95% CI 0.74–0.87), a perindopril-based regimen was associated with a significantly lower risk of the primary endpoint when compared to placebo. The additional treatment effect appeared numerically greater in non-statin users, but the observed difference was statistically nonsignificant.

Conclusion
Our data suggest that the treatment benefits of a perindopril-based regimen in patients with vascular disease are independent of statin use.
Social Memory in the Mekong’s Changing Floodscapes: Narratives of Agrarian Communities’ Adaptation
Authors: Tran Thong Anh , Rigg Jonathan , Taylor David , Miller Michelle Ann , Pittock Jamie , Le Phong Thanh 
Published: October 2022

AbstractRural adaptation encompasses place-based perceptions, behaviors, livelihoods, and traditional ways of life associated with local environments. These perceptions, norms, and practices are disturbed by coupled environment-development externalities. This study employs the Vietnamese Mekong floodplains as an exemplary case to illustrate how floods impact agrarian communities and how they have experienced flood alterations driven by hydropower development and climate change in recent years. Drawing on thematic and narrative analyses of qualitative data (focus group discussions and interviews) collected in three agrarian communities in the Vietnamese Mekong floodplains, sources drawn from various news outlets, and academic materials, we argue that disrupted flood environments in the floodplains have triggered affective flood reminiscences, catalysing shifts to incremental and transformative adaptation to achieve resilience. We build a nuanced understanding of how social memory helps to enhance human–environment relationships in response to highly complex hydrological dynamics in the delta.
Cost-based Optimization of Multistore Query Plans
Authors: Forresi Chiara , Francia Matteo , Gallinucci Enrico , Golfarelli Matteo 
Published: October 2022

AbstractMultistores are data management systems that enable query processing across different and heterogeneous databases; besides the distribution of data, complexity factors like schema heterogeneity and data replication must be resolved through integration and data fusion activities. Our multistore solution relies on a dataspace to provide the user with an integrated view of the available data and enables the formulation and execution of GPSJ queries. In this paper, we propose a technique to optimize the execution of GPSJ queries by formulating and evaluating different execution plans on the multistore. In particular, we outline different strategies to carry out joins and data fusion by relying on different schema representations; then, a self-learning black-box cost model is used to estimate execution times and select the most efficient plan. The experiments assess the effectiveness of the cost model in choosing the best execution plan for the given queries and exploit multiple multistore benchmarks to investigate the factors that influence the performance of different plans.
ANFIS for risk estimation in risk-based access control model for smart homes
Authors: Atlam Hany F. , Wills Gary B. 
Published: October 2022

AbstractThe risk-based access control model is one of the dynamic models that use the security risk as a criterion to decide the access decision for each access request. This model permits or denies access requests dynamically based on the estimated risk value. The essential stage of implementing this model is the risk estimation process. This process is based on estimating the possibility of information leakage and the value of that information. Several researchers utilized different methods for risk estimation but most of these methods were based on qualitative measures, which cannot suit the access control context that needs numeric and precise risk values to decide either granting or denying access. Therefore, this paper presents a novel Adaptive Neuro-Fuzzy Inference System (ANFIS) model for risk estimation in the risk-based access control model for the Internet of Things (IoT). The proposed ANFIS model was implemented and evaluated against access control scenarios of smart homes. The results demonstrated that the proposed ANFIS model provides an efficient and accurate risk estimation technique that can adapt to the changing conditions of the IoT environment. To validate the applicability and effectiveness of the proposed ANFIS model in smart homes, ten IoT security experts were interviewed. The results of the interviews illustrated that all experts confirmed that the proposed ANFIS model provides accurate and realistic results with a 0.713 in Cronbach’s alpha coefficient which indicates that the results are consistent and reliable. Compared to existing work, the proposed ANFIS model provides an efficient processing time as it reduces the processing time from 57.385 to 10.875 Sec per 1000 access requests, which demonstrates that the proposed model provides effective and accurate risk evaluation in a timely manner.
Stakeholder and Equity Data-Driven Implementation: a Mixed Methods Pilot Feasibility Study
Authors: Aschbrenner Kelly A. , Kruse Gina , Emmons Karen M. , Singh Deepinder , Barber-Dubois Marjanna E. , Miller Angela M. , Thomas Annette N. , Bartels Stephen J. 
Published: October 2022

AbstractWe conducted a mixed methods pilot feasibility study of a Stakeholder and Equity Data-Driven Implementation (SEDDI) process to facilitate using healthcare data to identify patient groups experiencing gaps in the use of evidence-based interventions (EBIs) and rapidly adapt EBIs to achieve greater access and equitable outcomes. We evaluated the feasibility and acceptability of SEDDI in a pilot hybrid type 2 effectiveness-implementation trial of a paired colorectal cancer (CRC) and social needs screening intervention at four federally qualified community health centers (CHCs). An external facilitator partnered with CHC teams to support initial implementation, followed by the SEDDI phase focused on advancing health equity. Facilitation sessions were delivered over 8 months. Preliminary evaluation of SEDDI involved convergent mixed methods with quantitative survey and focus group data. CHCs used data to identify gaps in outreach and completion of CRC screening with respect to race/ethnicity, gender, age, and language. Adaptations to improve access and use of the intervention included cultural, linguistic, and health literacy tailoring. CHC teams reported that facilitation and systematic review of data were helpful in identifying and prioritizing gaps. None of the four CHCs completed rapid cycle testing of adaptations largely due to competing priorities during the COVID-19 response. SEDDI has the potential for advancing chronic disease prevention and management by providing a stakeholder and data-driven approach to identify and prioritize health equity targets and guide adaptations to improve health equity. ClinicalTrials.gov Identifier: NCT04585919.
Diamonds are not forever: Adam Smith and Carl Menger on value and relative status
Authors: Hurtado Jimena , Paganelli Maria Pia 
Published: October 2022

AbstractThe differences on value between Smith and Menger may be other from previously alleged if status is included as a need satisfied only by truly scarce goods. Given our innate desire to distinguish ourselves and be admired, Smith tells us that scarcity is what generates value. So, our desire for distinction is what drives our demand for “diamonds” and gives them value. But when “diamonds” become as abundant as “knitted stockings”, new forms of scarce status goods must be found. For Menger, value is also a dynamic, evolving “institution” that cannot be understood in a static analysis dealing only with supply and demand at a given moment. But abundance, for Menger, would simply allow us to fulfill higher-level needs. Thus, Smith, but not necessarily Menger, could explain why, as diamond rings, Prada shoes, and Gucci bags can be easily mass produced in identical but “fake” copies, irreproducible goods, such as charity donations and claims on digital art, are taking their place to indicate status in society.
Points to consider in seeking biosafety approval for research, testing, and environmental release of experimental genetically modified biocontrol products during research and development
Authors: Tonui W. K. , Ahuja V. , Beech C. J. , Connolly J. B. , Dass B. , Glandorf D. C. M. , James S. , Muchiri J. N. , Mugoya C. F. , Okoree E. A. , Quemada H. , Romeis J. 
Published: October 2022

AbstractNovel genetically modified biological control products (referred to as “GM biocontrol products”) are being considered to address a range of complex problems in public health, conservation, and agriculture, including preventing the transmission of vector-borne parasitic and viral diseases as well as the spread of invasive plant and animal species. These interventions involve release of genetically modified organisms (GMOs) into the environment, sometimes with intentional dissemination of the modification within the local population of the targeted species, which presents new challenges and opportunities for regulatory review and decision-making. Practices developed for GMOs, primarily applied to date for GM crops may need to be adapted to accommodate different types of organisms, such as insects, and different technologies, such as gene drive. Developers of new GM biocontrol products would benefit from an early understanding of safety data and information that are likely to be required within the regulatory dossier for regulatory evaluation and decision making. Here a generalizable tool drawing from existing GM crop dossier requirements, forms, and relevant experience is proposed to assist researchers and developers organize and plan their research and trialing. This tool requires considering specifics of each investigational product, their intended use, and country specific requirements at various phases of potential product development, from laboratory research through contained field testing and experimental release into the environment. This may also be helpful to risk assessors and regulators in supporting their systematic and rigorous evaluation of new biocontrol products.
Schmerztherapie mit „eye movement desensitization and reprocessing“ (EMDR): eine Pilotstudie zur Verbesserung der Lebensqualität
Authors: Weiß Katharina , Kohls Niko 
Published: October 2022

Zusammenfassung
Hintergrund
Ein effektives Schmerzmanagement mit langandauernder Wirkung bei Menschen mit chronischen Schmerzen ist noch immer eine Herausforderung.

Ziel der Arbeit
Ziel der Arbeit ist die Untersuchung der Effekte einer gesundheitsförderlichen Intervention mit EMDR („eye movement desensitization and reprocessing“) bei Personen mit chronischen Schmerzen und eine mögliche Verbesserung ihrer Lebensqualität.

Methodik
Mit Hilfe einer Paper-pencil-Befragung wurden 30 Schmerzpatient:innen zu zwei Messzeitpunkten (MZP) mit Subskalen des Deutschen Schmerzfragebogens (DSF), dem Pain Disability Index (PDI) und der WHO Quality of Life-Kurzversion (WHOQOL-BREF) befragt. Zwischen den MZP wurden die Personen der Interventionsgruppe mit EMDR behandelt.

Ergebnisse
Einige signifikante Ergebnisse deuten darauf hin, dass durch die Intervention mit EMDR zur Behandlung chronischer Schmerzen die Lebensqualität verbessert wird. So verbesserte sich in der Interventionsgruppe die Lebensqualität in den Domänen physische, psychische und globale Lebensqualität signifikant im Vergleich beider MZP.

Schlussfolgerung
Da die erhobenen Befunde aufgrund des explorativen Studiencharakters nur unter Vorbehalt interpretiert werden können, sollten in Zukunft Langzeitstudien mit größeren Stichproben und Follow-up-Erhebungen durchgeführt werden, um eindeutige Aussagen über die Wirksamkeit und die Langzeiteffekte von EMDR zur Behandlung chronischer Schmerzen treffen zu können. In der Behandlungsmethode dürfte großes Potenzial liegen und es scheint vielversprechend zu sein, EMDR zu einem festen Bestandteil einer multimodalen, interdisziplinären Schmerztherapie werden zu lassen.
A compactness result for the div-curl system with inhomogeneous mixed boundary conditions for bounded Lipschitz domains and some applications
Authors: Pauly Dirk , Skrepek Nathanael 
Published: October 2022

AbstractFor a bounded Lipschitz domain with Lipschitz interface we show the following compactness theorem: Any $$\mathsf {L}_{}^{2}$$

L

2

-bounded sequence of vector fields with $$\mathsf {L}_{}^{2}$$

L

2

-bounded rotations and $$\mathsf {L}_{}^{2}$$

L

2

-bounded divergences as well as $$\mathsf {L}_{}^{2}$$

L

2

-bounded tangential traces on one part of the boundary and $$\mathsf {L}_{}^{2}$$

L

2

-bounded normal traces on the other part of the boundary, contains a strongly $$\mathsf {L}_{}^{2}$$

L

2

-convergent subsequence. This generalises recent results for homogeneous mixed boundary conditions in Bauer et al. (SIAM J Math Anal 48(4):2912-2943, 2016) Bauer et al. (in: Maxwell’s Equations: Analysis and Numerics (Radon Series on Computational and Applied Mathematics 24), De Gruyter, pp. 77-104, 2019). As applications we present a related Friedrichs/Poincaré type estimate, a div-curl lemma, and show that the Maxwell operator with mixed tangential and impedance boundary conditions (Robin type boundary conditions) has compact resolvents.
Overview of Moving Particle Semi-implicit Techniques for Hydrodynamic Problems in Ocean Engineering
Authors: Xie Fengze , Zhao Weiwen , Wan Decheng 
Published: October 2022

AbstractWith the significant development of computer hardware, many advanced numerical techniques have been proposed to investigate complex hydrodynamic problems. This article aims to provide a detailed review of moving particle semi-implicit (MPS) techniques and their application in ocean and coastal engineering. The achievements of the MPS method in stability and accuracy, boundary conditions, and acceleration techniques are discussed. The applications of the MPS method, which are classified into two main categories, namely, multiphase flows and fluid-structure interactions, are introduced. Finally, the prospects and conclusions are highlighted. The MPS method has the potential to solve practical problems.
Numerical Investigation on the Deformation of the Free Interface During Water Entry and Exit of a Circular Cylinder by Using the Immersed Boundary-Multiphase Lattice Boltzmann Flux Solver
Authors: Zhang Guiyong , Yan Haoran , Song Hong , Wang Heng , Hui Da 
Published: October 2022

AbstractIn this work, the deformation of free interface during water entry and exit of a circular cylinder is investigated numerically by using the two-dimensional (2D) immersed boundary-multiphase lattice Boltzmann flux solver (IB-MLBFS). The fluid domain is discretized by finite volume discretization, and the flux on the grid interface is evaluated by lattice Boltzmann equations. Both the implicit velocity correction and the surface flux correction are implemented by using the immersed boundary-method to consider the fluid-structure interaction and the contact interface between the multiphase fluids and the structure. First, the water entry of a circular cylinder is simulated and the results are compared with the experiment, which considered the length-diameter ratio of the circular cylinder. The reliability of 2D simulation is verified and the deformation of the free interface is well investigated. Afterward, the water exit of a circular cylinder with constant velocity is simulated, which is less researched. In addition, the results show the advantage of present IB-MLBFS to some extent. Finally, the water exit and re-entry of a circular cylinder are presented, and the results present the complex deformation of the free interface and the dynamic response of the moving structure. Based on the numerical results, the free interface of the multiphase fluids is well captured, and the contact interface on the boundary of the moving structure is accurately presented by the IB-MLBFS.