bluuebunny/crossref_metadata_2025_split huggingface dataset:
Roles of BrlA and AbaA in Mediating Asexual and Insect Pathogenic Lifecycles of Metarhizium robertsii
Authors: Zhang Jin-Guan , Xu Si-Yuan , Ying Sheng-Hua , Feng Ming-Guang 
Published: October 2022

BrlA and AbaA are key activators of the central developmental pathway (CDP) that controls asexual development in Aspergillus but their roles remain insufficiently understood in hypocerealean insect pathogens. Here, regulatory roles of BrlA and AbaA orthologs in Metarhizium robertsii (Clavicipitaceae) were characterized for comparison to those elucidated previously in Beauveria bassiana (Cordycipitaceae) at phenotypic and transcriptomic levels. Time-course transcription profiles of brlA, abaA, and the other CDP activator gene wetA revealed that they were not so sequentially activated in M. robertsii as learned in Aspergillus. Aerial conidiation essential for fungal infection and dispersal, submerged blastospore production mimicking yeast-like budding proliferation in insect hemocoel, and insect pathogenicity via cuticular penetration were all abolished as a consequence of brlA or abaA disruption, which had little impact on normal hyphal growth. The disruptants were severely compromised in virulence via cuticle-bypassing infection (intrahemocoel injection) and differentially impaired in cellular tolerance to oxidative and cell wall-perturbing stresses. The ΔbrlA and ΔabaA mutant shad 255 and 233 dysregulated genes (up/down ratios: 52:203 and 101:122) respectively, including 108 genes co-dysregulated. These counts were small compared with 1513 and 2869 dysregulated genes (up/down ratios: 707:806 and 1513:1356) identified in ΔbrlA and ΔabaA mutants of B. bassiana. Results revealed not only conserved roles for BrlA and AbaA in asexual developmental control but also their indispensable roles in fungal adaptation to the insect-pathogenic lifecycle and host habitats. Intriguingly, BrlA- or AbaA-controlled gene expression networks are largely different between the two insect pathogens, in which similar phenotypes were compromised in the absence of either brlA or abaA.
The Brahmavarta Initiative: A Roadmap for the First Self-Sustaining City-State on Mars
Authors: Mukundan Arvind , Wang Hsiang-Chen 
Published: October 2022

The vast universe, from its unfathomable ends to our very own Milky Way galaxy, is comprised of numerous celestial bodies—disparate yet each having their uniqueness. Amongst these bodies exist only a handful that have an environment that can nurture and sustain life. The Homo sapiens species has inhabited the planet, which is positioned in a precise way—Earth. It is an irrefutable truth that the planet Earth has provided us with all necessities for survival—for the human race to flourish and prosper and make scientific and technological advancements. Humans have always had an innate ardor for exploration—and now, since they have explored every nook and corner of this planet, inhabiting it and utilizing its resources, the time has come to alleviate the burden we have placed upon Earth to be the sole life-sustaining planet. With limited resources in our grasp and an ever-proliferating population, it is the need of the hour that we take a leap and go beyond the planet for inhabitation—explore the other celestial objects in our galaxy. Then, however, there arises a confounding conundrum—where do we go? The answer is right next to our home—the Red Planet, Mars. Space scientists have confirmed that Mars has conditions to support life and is the closest candidate for human inhabitation. The planet has certain similarities to Earth and its proximity provides us with convenient contact. This paper will be dealing with the conceptual design for the first city-state on Mars. Aggregating assumptions, research, and estimations, this first settlement project shall propose the most optimal means to explore, inhabit and colonize our sister planet, Mars.
Comparison of quantitative and qualita- tive characteristics of the system von Willebrand factor — metalloprotease ADAMTS13 in patients with acute myocardial in farction and is chemic stroke
Authors: Beliaeva E. L. , Koloskov A. V. , Gutkin I. M. , Philippova O. I. , Chernova E. V. , Kurnikova E. A. , Dorofeeva G. B. 
Published: October 2022

Introduction. The hemostasis system plays a key role in the pathogenesis of cardiovascular diseases.Aim — to evaluate changes in quantitative and qualitative characteristics of ADAMTS13 metalloprotease and von Willebrand factor an tigen (vWF:Ag) in patients with acute myocardial in farction (AMI) and is chemic stroke (IS).Patients and methods. The study in cluded 110 patients aged 42–95 years (median age — 64 years), of which: 65 patients with AMI aged 42–81 years (median age — 56 years) who had coronary artery thrombosis during an giography, as well as 45 patients with IS aged 40–95 years (median age — 70 years) with diagnosed cerebral artery thrombosis. All patients were tested for ADAMTS13 an tigen, ADAMTS13 activity, an tibodies to ADAMTS13 and vWF:Ag. Blood samples were obtained during an giography (arterial and venous blood) and 24 and 120 hours after an giography (venous blood).Results. In the group of patients with AMI, an in crease in the values of the vWF:Ag in dicator was revealed, which went beyond the upper limit of the reference in terval. In patients with IS, at the beginning of the study, this in dicator was within the reference in terval and was statistically signiﬁcantly lower compared to patients with AMI but in creased after 24 hours and peaked at 120 hours. When an alyzing changes in the ADAMTS13:Ag in dicator, it was found that it was statistically signiﬁcantly higher at all points of the study in the group of patients with AMI, compared with the group of patients with IS, however, the values of this in dicator did not go beyond the reference in terval in patients of both groups. At the same time, the ADAMTS13:AC in dex at all points of the study was statistically signiﬁcantly higher in patients with IS, compared with the group of patients with AMI, and the ADAMTS13:AB in dex. On the contrary, in the group of AMI patients, this in dicator was statistically signiﬁcantly higher in all points of the study when compared with that for the group of patients with IS.Conclusion. The study of two pathogenetic models of thrombosis demonstrated the absence of a relationship between the vWF:Ag in dex and the quantitative or qualitative characteristics of the ADAMTS13 metalloprotease. At the same time, an in crease in ADAMTS13:AB in AMI can be considered as the reason for the decrease in ADAMTS13:AC in these patients.
Combination of ibrutinib and venetoclax followed by Chimeric Antigen Receptor T-cell therapy in the ﬁrst line of treatment in an elderly patient with mantle cell lymphoma with hyperleukocytosis and mutation in the TP53 gene
Authors: Zvonkov E. E. , Koroleva D. A. , Gabeeva N. G. , Shchekina A. E. , Telyashov M. A. , Aleshina O. A. , Biderman B. V. , Sudarikov A. B. , Obukhova T. N. , Galtseva I. V. , Dvirnik V. N. , Troitskaya V. V. , Galstyan G. M. , Maschan M. A. , Parovichnikova E. N. 
Published: October 2022

Introduction. The tactics of therapy for elderly comorbid patients with mantle cell lymphoma with unfavorable prognosis factors (complex karyotype, 17p13 deletion, mutations in the TP53 gene) have not been developed. The use of intensive chemotherapy regimens and transplantation of allogeneic hematopoietic stem cells (allo-HSCT) is impossible due to severe comorbidity in elderly patients. A rational approach is the use of a combination of ibrutinib and venetoclax. As an alternative to allo-HSCT, a new option for elderly patients with poor prognostic factors is Chimeric Antigen Receptor T-cell therapy (CAR-T) cell therapy.Aim — to present the experience of using ibrutinib and venetoclax with CAR-T-cell therapy in the ﬁrst line of treatment in an elderly patient with MCL with a mutation in the TP53 gene and hyperleukocytosis.Main ﬁndings. Patient M., 68 years old. The examination revealed hyperleukocytosis 978 × 109/L, anemia (55 g/L), thrombocytopenia (30 × 109/L), and splenomegaly 250 × 180 mm. According to the results of laboratory studies, the diagnosis of lymphoma from mantle cells with a complex karyotype, deletion 17p13, 13q14 and mutation p.R248W in exon 7 of the TP53 gene (VAF = 26 %) was veriﬁed. For cytoreductive purposes, two sessions of leukocytapheresis and prephase with cyclophosphamide (200 mg/m2) and dexamethasone (10 mg/m2) were performed. From day 3, therapy with ibrutinib 420 mg/day and venetoclax 100 mg/day was started. After 2 days, the leukocytes were 0.7 × 109/L, and the size of the spleen decreased, as a result of which the development of tumor lysis syndrome was noted. As a result of intensive therapy, the patient’s condition stabilized, which allowed him to resume treatment. After 7 days, the number of leukocytes was 2.5 × 109/L, neutrophils — 70 %, platelets — 90 × 109/L, hemoglobin — 95 g/L. According to immunophenotyping, the population of B-lymphocytes was 4 %. According to NGS data, the allelic load of the mutation in the TP53 gene is 0.8 %. The patient underwent anti-CD19 CAR-T-cell therapy and achieved complete remission. Three months after therapy, MRD remains-negative remission and the persistence of CAR-T cells is determined.
Solvent-dependent dispersion stability of PTCDI-C13 nanoparticle colloids made by PLAL
Authors: Jeon Hyeon-Gu , Haramiishi Shuya , Asahi Tsuyoshi , Fujii Masaharu , Ihori Haruo 
Published: October 2022

Abstract
Fabrication of nanoparticle colloids of organic semiconductors is a fascinating subject not only because of its unique properties from the size-effects but also because of its practical advantage of low manufacturing cost for various electronic devices. The detail of its dispersion stability, however, is not studied properly, especially for organic colloids fabricated by pulsed laser ablation in liquid (PLAL) technique. In this study, the primary factor of stabilizing nanoparticles of N,N’-ditridecyl-3,4,9,10-perylenetetracarboxylic diimide (PTCDI-C13) during PLAL is determined by observing the dispersion stability of its colloids prepared in various organic solvents. It is clearly proved that the functional group of solvents is the decisive factor for the dispersion stability of PTCDI-C13 colloids. This result can be applied to other organic semiconductors and promote the advent of a new methodology of organic electronics utilizing highly stable nanoparticle colloids of organic semiconductors for low-cost fabrication of electronic devices.
Pengembangan Art Furniture Pada 168 Madura Art Di Yogyakarta
Authors: Faishal Hakim Mochammad 
Published: October 2022

Penelitian ini bertujuan untuk mengetahui kemampuan pengembangan art furniture pada 168 madura art di yogyakarta. Penelitian ini merupakan penelitian deskriptif kuantitatif yaitu penelitian yang berusaha untuk menututurkan pemecahan masalah yang ada sekarang dengam menggunakan angka, mulai dari pengumpulan data, penafsiran terhadap data tersebut serta penampilan dari hasilnya. Teknik pengumpulan data yang digunakan dalam penelitian ini menggunakan teknik dokumentasi. Data yamg digunakan adalah data sekunder berupa laporan perkiraan target rugi laba atau r/l tahun 2020 dan laporan realisasi rugi/laba atau r/l tahun 2020 yang membentuk laporan pencapaian perbandingan target dan realisasi rugi/laba atau r/l tahun2020. Setelah melakukan analisis, hasil penelitian menunjukkan ditahun 2020, hasil didapat antara target dan realisasinya untuk laba bersih yang diperoleh ada selisih rp.152.865.005,- - rp.112.612.505,- =rp.40.252.500,- dari total hasil laporan laba/rugi dalam setahun atau 2020 ( 26,33 % ), realisasi laba bersih diperoleh hanya kisaran 73,667 %. Awal tahun 2020 realisasi laba bersih dari penjualan hanya kisaran 42,2 % dalam tabel 4.6 di antara bulan maret 2020 sampai april 2020, untuk bulan mei 2020 sampai desember 2020 fluktuatif mulai naik realisasinya dari 42,2%. Dari hasil tabel : menunjukkan tahun awal operasional realisasi r/l tahun 2020 dihitung rata rata dari target yang ditetapkan tahun 2020 baru mencapai kisaran 72,98.%.
Upaya Edukasi Kader tentang Kebersihan Daerah Kewanitaan untuk Mencegah Keputihan
Authors: Suptiani Laila Putri , Rohmatin Etin 
Published: October 2022

an kegiatan pengabdian masyarakat ini adalah untuk meningkatkan pengetahuan kader tentang kebersihan daerah kewanitaan pada kasus keputihan. Metode yang dilaksanakan adalah melalui pemberian informasi kesehatan dan pendampingan dari Tim Dosen dan dibantu oleh mahasiswa Jurusan Kebidanan Poltekkes Kemenkes Tasikmalaya di Wilayah Kerja Puskesmas Kahuripan Kelurahan Cikalang Kota Tasikmalaya. Adapun yang menjadi sasaran dalam kegiatan ini yaitu kader di Kelurahan Cikalang wilayah kerja Puskesmas Kahuripan Kota Tasikmalaya. Hasil pelaksanaan didapatkan bahwa meningkatnya pengetahuan kader tentang kebersihan daerah kewanitaan pada kasus keputihan. Simpulan dari kegiatan ini adalah upaya edukasi kader tentang kebersihan daerah kewanitaan untuk mencegah keputihan melalui pemberian buku saku didapatkan hasil pengetahuan kader yang meningkat.
Awareness of Palliative Care among Healthcare Providers in Pakistan: A Survey
Authors: Kashif Sanum 
Published: October 2022

Objective: To assess the awareness of palliative care among healthcare providers in Pakistan.Study Design: Cross-sectional descriptive study.Place and Duration of Study: The study was conducted in healthcare providers from various healthcare setupsin Pakistan, from 20th March 2021 to 21st May 2021.Materials and Methods: A total of 134 responders from various hospitals answered a questionnaire-basedsurvey on the knowledge and palliative care practice among healthcare providers. The questionnaire was madeaccording to guidelines given By Shaheen Palliative Care Project, United Kingdom.For data collection, convenience sampling was used and to present distribution of responses percentages andfrequencies were used. Data were entered and analyzed using SPSS 17.Result: In 134 responders, about 128 (95.5%) responders were involved in palliative care, but only 55 (41.0%)were satisfied with palliative care given. Majority of responders 118 (88.0%) had idea regarding members ofpalliative care team. About 124 (73.4%) responders knew that, palliative care should start at the time ofdiagnosis and 123 (91.7%) gave correct response regarding the goal of palliative care. Most of the responders124 (92.5%) were convinced that palliative care should be the part of curriculum at all health care levels.Conclusion: Among healthcare providers across Pakistan, the majority have fair idea regarding palliative carebut a more than half of the responders were not satisfied with palliative care they are providing which is mainlydue to the lack in palliative medicine training. Palliative care should be the part of curriculum at all health carelevels with more online programs and workshops.
How to cite this: Kashif S. Awareness of Palliative Care among Healthcare Providers in Pakistan: A Survey. 2022; 3(4): 174-177. doi: http://doi.org/10.37185/LnS.1.1.224
The Effect of Perceived Stress and Body Mass Index on Premenstrual Dysphoric Disorder among Female University Students
Authors: Hameed Muddsar , Fatima Mishal 
Published: October 2022

Objective: This study aims to assess perceived stress and Body Mass Index (BMI) on premenstrual dysphoricdisorder symptoms among young female university students. University life contains a lot of stress, includingexams, career and other interpersonal relationships with colleagues and teachers. The current study aims tofind the association between perceived stress and Premenstrual Dysphoric Disorder (PMDD).Study Design: A cross-sectional quantitative study.Place and Duration of Study: The study was conducted at the Department of Clinical Psychology Shifa Tameere-Millet University Islamabad from February 2021 to June 2021. Data was collected from different universities in Islamabad, which include Shifa Tameer-e-Millet University, Bahria University, Islamic university, Air University, NUST, and Quaid-e-Azam University.Materials and Methods: An online survey was carried out among female university students in the universitiesof Islamabad. A Questionnaire was developed, which comprised three separate sections. Demographics sheetswere used for demographic variables. Premenstrual dysphoric disorder symptoms were assessed based on thescoring of the Carolina Premenstrual Dysphoric Disorder Scale. A perceived stress scale is used to measurestress levels. Multiple linear regression analysis was used to assess the effects of variables. BMI was calculatedwith the formula of WHO.Results: The study was conducted on 210 female university students of the Islamabad age range (18-30). Theresults of the regression analysis indicated that two predictors of this study explained 34% of the variance in our 2 outcome variable (R2 =.349). It was found that perceived stress, but not body mass index, was a significantpredictor of premenstrual dysphoric disorder (ß = 0.582, p<.05), whereby for each unit increase in perceivedstress, the premenstrual dysphoric disorder increased by 0.58 units.Conclusion: This study has shown that young female adults become more vulnerable to developing symptomsof premenstrual dysphoric disorder due to perceived stress which may affect academic, financial, and familialrelationships. The female adults with these symptoms should be addressed by providing them with medicaland psychological support.
How to cite this: Hameed M, Fatima M. The Effect of Perceived Stress and Body Mass Index on Premenstrual Dysphoric Disorder among Female University Students. Life and Science. 2022; 3(4): 178-182. doi: http://doi.org/10.37185/LnS.1.1.227
Deep Learning–based Search for Microlensing Signature from Binary Black Hole Events in GWTC-1 and -2
Authors: Kim Kyungmin , Lee Joongoo , Hannuksela Otto A. , Li Tjonnie G. F. 
Published: October 2022

Abstract
We present the result of the first deep learning–based search for the signature of microlensing in gravitational waves. This search seeks the signature induced by lenses with masses between 103 and 105
M
⊙ from spectrograms of the binary black hole events in the first and second gravitational-wave transient catalogs. We use a deep learning model trained with spectrograms of simulated noisy gravitational-wave signals to classify the events into two classes, lensed or unlensed. We introduce ensemble learning and a majority voting-based consistency test for the predictions of ensemble learners. The classification scheme of this search primarily classifies one event, GW190707_093326, into the lensed class. To verify the primary classification of this event, we also examine the median probability of the lensed class and observe that the resulting value,

0.984

−
0.342

+
0.012

, agrees with an empirical criterion >0.6 for claiming the detection of a lensed signal. However, the uncertainty of the estimated p-value for the median probability and error, ranging from 0 to 0.1, convinces us GW190707_093326 is less likely a lensed event because it includes p ≥ 0.05 where the unlensed hypothesis is true. Therefore, we conclude our search finds no significant evidence of microlensing signature from the evaluated binary black hole events.
An experimental research on emotional stimuli of consumers: The case of H&M flagship store on the millennial customer experience in Barcelona
Authors: Regadera González Elisa , Selvam Rejina M. , García Medina Irene , González Romo Zahaira Fabiola 
Published: October 2022

Purpose: The main objective of this exploratory research study was to demonstrate the extent to which customers' approach-avoidance responses to fast fashion flagship stores can be predicted from their emotional states framed according to the PAD (Pleasure, Arousal, and Dominance) model and information rates.Design/methodology/approach: Data was collected through a survey questionnaire from 72 randomly selected millennials visiting H&M flagship store, just after their visit (55% women and 45% men). Data was analysed using exploratory factor analysis to classify the measures and later through regression analysis explaining the predictor variables.Findings: In accordance with the theory and past research on PAD measures, results show PAD as a highly predictable variable over most of the approach-avoidance behaviour in a flagship store environment. Pleasure variable shows highly significant relationship with affect, approach-avoidance (average of all measures of approach-avoidance), and time spent in the store and to an extent significantly relates to purchase intentions. This signifies that in a highly pleasant environment, an individual’s affectionate behaviour, to remain exploring in the store for a longer time, and on an average the approach behaviour to the flagship store will be highly positive. Originality/value: This study is a unique exploration, as very little is known in the literature, on flagship store influences over consumer experiences. Findings suggest interesting advancement in the field of in person shopping effects and encourages future research in flagship store economic investments and marketing impacts, which may extend consumer behavioural scopes as well. This exploratory research may have implications of interest for business strategic decision, brand management, and for marketing as well. As brands seek to recover consumers in their physical stores, offering them a safer and more complete shopping experience, flagship stores can play a key role in encouraging online customers’ visiting stores in-person, which may ultimately increase sales.
Enhancing Doctor of Nursing Practice Students' Diagnostic and Clinical Reasoning Skills
Authors: Conner Ruthie , Cormack Carrie , Fowler Terri , Durham Cathy 
Published: October 2022

Background:
This article describes one school of nursing's successful approach to augmenting Doctor of Nursing Practice students' diagnostic and clinical reasoning skills.

Method:
Faculty convened a taskforce to evaluate the current clinical tracking system to expand the clinical log and develop a formative assessment tool.

Results:
The clinical log enhancement allowed faculty to assess students' learning, clinical decision making, and readiness for clinical progression throughout the program.

Conclusion:

The expansion of the clinical log system resulted in students' heightened understanding of documentation and clinical practice, and also allowed faculty to comprehensively assess students in diagnostic and clinical reasoning.

[
J Nurs Educ
. 2023;62(2):109–111.]
Henri Pichette et le « trésor » de l’enfance : de la collecte au poème
Authors: Pardo Céline 
Published: October 2022

Résumé : On relève dans l’œuvre du poète Henri Pichette quelques textes empruntant directement leur forme aux traditions orales enfantines : des comptines et des formulettes dans la version finale des Épiphanies (dont la réécriture est entamée en 1965), des « enfantines » publiées en marge de l’œuvre proprement dite (dans les Cahiers Henri Pichette en 1991 et 1995), des « rimettes et amusettes » dans Les Ditelis du rougegorge (livre posthume paru en 2005). Ces formes qu’investit le poète à partir du milieu des années 1960, après son séjour au Canada, sont à mettre en relation avec son étude passionnée, à partir de la même époque, des parlers populaires, des patois et des dialectes, mais aussi des travaux folkloristes dans lesquels il recueille en particulier tout ce qui se rapporte de noms, dictons, expressions populaires, mimologismes ou récits légendaires à la figure du rouge-gorge. Lui-même conduit différentes enquêtes de terrain sur cet oiseau dès la fin des années 1970. À partir de cet oiseau devenu pour lui un véritable objet de dévotion, tant spirituel que poétique, Pichette engage son écriture dans une poétique de l’« enfance », visant non seulement à ranimer les croyances ancestrales contenues dans la langue, mais aussi à rendre au lecteur le « trésor » perdu de l’enfance : cet enchantement devant les mots qui, de simple plaisir ludique ou érudit, peut aussi conduire à un réenchantement mystique du réel.