bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the antimicrobial properties of Staphylococci-derived compounds against ESKAPE bacteria
Authors: Modimola Margaret Selina , Njobeh Patrick Berka , Senabe Jeremiah , Fouche Gerda , McGaw Lyndy , Makhubu Fikile Nelly , Mathiba Kgama , Mthobeni Julian , Green Ezekiel 
Published: December 2022

Abstract
Klebsiella pneumoniae, Enterococcus faecium, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter cloacae, and Enterobacter aerogenes (ESKAPE) bacterial group is well noted for drug resistance. These strains cause various diseases and in doing so, utilize various ways to resist a broad range of antibiotics. Susceptibility to antibiotics amongst ESKAPE bacteria is rapidly declining, and therefore, there is a need to explore alternative ways to deal with ESKAPE-related conditions by utilizing bacterial compounds. The compounds used in this study were derived from secondary metabolites of Staphylococci stains (S saprophyticus [ATCC 35552], S. aureus [isolated from milk of mastitic cows], and S. epidermidis [ATCC 51625]. These compounds were screened, identified and evaluated for cytotoxicity beforehand. Such compounds included Veratramine, 1,2,6-Hexanetriol, Succinic acid, 4-Methyl-pentyl-amine; from S. saprophyticus, Fluoranthene, 3-Methyl-2-phenyl-1H-pyrrole, and Cyclo (L-Leu-L-Propyl) and from S. epidermidis, Oleamide and Methyl palmitate. However, Fluoranthene is excluded in this study as it previously exhibited cytotoxicity against Vero cells, therefore, is considered unsuitable as a prospective antimicrobial agent. Prior to exploring the antimicrobial properties of Staphylococci-derived compounds, the susceptibility of ESKAPE bacteria was tested against the antibiotic regimens as per South African guidelines. The investigations on antibiotics susceptibility were carried out to compare the susceptibility of ESKAPE bacteria to known antibiotics against Staphylococci-derived compounds. The antimicrobial properties of the compounds were evaluated both qualitatively and quantitatively by TLC bioautography and a broth microdilution method, respectively. In this study, we found that all the compounds did not exhibit any antimicrobial properties against the ESKAPE bacteria except for Succinic acid, which exhibited its efficacy against A. baumannii with a minimum inhibitory concentration (MIC) of 62.5 µg/mL.
CD47 antisense oligonucleotide treatment attenuates obesity and its-associated metabolic dysfunction
Authors: Gwag Taesik , Li Dong , Ma Eric , Guo Zhenheng , Liang Ying , Wang Shuxia 
Published: December 2022

Abstract
Background: CD47 is a ubiquitously expressed cell membrane receptor implicated in self-recognition and immune function. Previous study from our lab has revealed a new role of CD47 in regulating adipose tissue function and energy homeostasis. Genetic deletion of CD47 has been shown to protect mice from diet-induced obesity and its-associated metabolic complications. In this study, the therapeutic potential of an antisense oligonucleotide (ASO) targeting to CD47 in obesity and metabolic disease was determined.
Methods: Two obese mouse models (diet induced obese mice and leptin deficient ob/ob mice) were utilized. For diet induced obesity, male C57BL6 mice were fed with high fat (HF) diet for 6 weeks to induce obesity and glucose intolerance. Then these mice were treated with CD47ASO or control ASO for 8 weeks with continuous HF diet feeding. For genetic obese mouse model, male six-week old ob/ob mice were treated with ASOs for 9 weeks. Body weight was monitored weekly. By the end of study, glucose tolerance test, indirect calorimetry, body composition and cold exposure experiments were performed.
Results: We found that CD47ASO treatment reduced HF diet-induced weight gain, decreased fat mass, prevented dyslipidemia, and improved glucose tolerance. These changes were accompanied by reduced inflammation in white adipose tissue, reduced hepatic lipogenic gene expression and decreased hepatic steatosis. This protection was also seen in CD47ASO treated ob/ob mice. CD47ASO treated mice displayed increased energy expenditure. Interestingly, these mice displayed increased voluntary wheel running distance in metabolic cage, which was associated with increased lean mass and expression of uncoupling proteins (UCP2 and UCP3) in skeletal muscle. Food intake or cold tolerance was comparable between control ASO and CD47 ASO treated mice.
Conclusions: These findings suggest that CD47ASO might serve as a new treatment option for obesity and its-associated metabolic complications.
Characterization and commissioning of a new collaborative multi-modality radiotherapy platform
Authors: Wang Zhongfei , Sun Xiaohuan , Wang Wei , Zhang Te , Chen Liting , Duan Jie , Feng Siqi , Chen Yinzhu , Wei Zhiwei , Zang Jian , Xiao Feng , Zhao Lina 
Published: December 2022

Abstract
Purpose: TaiChi, a new multi-modality radiotherapy platform that integrates a linear accelerator, a focusing gamma system, and a kV imaging system within an enclosed O-ring gantry, was introduced into clinical application. This work aims to assess the technological characteristics and commissioning results of the TaiChi platform.
Methods: The acceptance testing and commissioning were performed following the manufacturer’s customer acceptance tests (CAT) and several AAPM Task Groups (TG) reports/guidelines. Regarding the linear accelerator(linac), all applicable validation measurementsrecommended by the MPPG 5.a (basic photon beam model validation, intensity-modulated radiotherapy (IMRT)/volumetric-modulated arc therapy (VMAT) validation, end-to-end(E2E) tests, and patient-specific quality assurance (QA)) were performed. For the focusing gamma system, the absorbed doses were measured using a PTW31014 ion chamber (IC) and PTW60016 diode detector. EBT3 films and a PTW60016 diode detector were employed to measure the relative output factors (ROFs). The E2E tests were performed using PTW31014 IC and EBT3 films. The coincidences between the imaging isocenter and the linac/gamma treatment isocenter were investigated using EBT3 films. The image quality was evaluated regarding the contrast-to-noise ratio (CNR), spatial resolution, and uniformity.
Results: All tests included in the CAT met the manufacturer’s specifications. All MPPG 5.a measurements complied with the tolerances. The confidence limits for IMRT/VMAT point dose and dose distribution measurements were achieved according to TG-119. The point dose differences were below 1.68% and gamma passing rates were above 95.9% for the linac E2E tests. All plans of patient-specific QA had point dose differences below 1.79% and gamma passing rates above 96.1% using the 3%/2 mm criterion suggested by TG-218. For the focusing gamma system, the differences between the calculated and measured absorbed doses were below 1.86%. The ROFs calculated by the TPS were independently confirmed within 2% using EBT3 films and a PTW60016 detector. The point dose differences were below 2.57% and gamma passing rates were above 95.3% using the 2%/1 mm criterion for the E2E tests. The coincidences between the imaging isocenter and the linac/gamma treatment isocenter were within 0.5 mm. The image quality parameters fully complied with the manufacturer’s specifications regarding the CNR, spatial resolution, and uniformity.
Conclusion: The multi-modality radiotherapy platform complies with the CAT and AAPM commissioning criteria. The commissioning results demonstrate that this platform performs well in mechanical and dosimetry accuracy.
Assessment of Groundwater Quality Status by using Hydrochemical and GIS approach for the feasible study of Coastal Reservoirs
Authors: Suryawanshi Vijay , H Ramesh , Nasar T 
Published: December 2022

Abstract
Groundwater qualities of coastal aquifers in the Netravati and Gurapura catchment of Dakshina Kannada district Karnataka have been extensively monitored in post-monsoon seasons in 2021 and Pre-Monsoon 2022 to assess their suitability for domestic and drinking uses in four regions. Thirty-two groundwater samples were analysed for various physicochemical parameters such as GWL, pH, Oxidation Reduction Potential (ORP), Electrical Conductivity (EC), Total Hardness (TH), Dissolved Oxygen (DO), Total Dissolved solids (TDS), Temperature, Calcium (Ca), Magnesium (Mg), Chloride (Cl), Sodium (Na), Potassium (K), Sulphate (SO4) and Carbonate and Bicarbonate. Most of these parameters fall under the permissible limits of BIS and WHO standards. Using hydrochemistry and the GIS method, the current study aims to evaluate the quality of groundwater (well water samples) in the Netravati river basin. The quality of the groundwater, indicates that coastal aquifers have gained prominence over the past ten years as a result of the using global demand for groundwater. According to the findings of the study, groundwater depletion and the rising seawater level are the primary factors that contribute to the saline condition of coastal wells during the pre-monsoon period. The study demonstrates that groundwater levels have been trending downward for some time. Seawater intrusion appears to be the main problem in the study area's coastal region, according to the study. The water quality index (WQI) results show that the quality of well water samples during the pre-monsoon session is much worse than during the post-monsoon session due to fresh rainwater during the post-monsoon session raises the groundwater level and increases the water's physical and chemical parameters. Based on the recommendations of the Hortons water quality index, groundwater quality is divided into four categories: excellent water quality (90-100), good water quality (71-90), poor water quality (51-70), and bad water quality (31-51).
Post Evaluation of Adaptive reuse of Architectural Heritage: A Case Study of Beijing Central Axis Historical Buildings
Authors: Yan Zhang , Zhang Qi 
Published: December 2022

Abstract
The reuse of architectural heritage is the basis of embodying its core value, bringing new life to heritage architecture, and is an important way to integrate it into contemporary development. In many current studies, the reuse of architectural heritage focuses on the research framework of reuse and the most effective method for the reuse of a certain building. In the whole process, the post evaluation strategy of reuse based on specific cultural background is ignored, and the reuse of heritage buildings is a dynamic process. Therefore, this study takes 9 heritage sites along the central axis of Beijing (including three types of single buildings, architectural complexes, and historical and cultural blocks) as examples, uses literature retrieval, analytic hierarchy process, and fuzzy comprehensive evaluation to extract the factors that affect the reuse of architectural heritage, and provides evaluation methods and strategies for the reuse of architectural heritage. Finally, a post evaluation framework for the reuse of architectural heritage is formed, which guides the relevant decision-making and optimization process.
Design, synthesis and Neuraminidase inhibitory activity of 4-methyl-5-(3-phenylacryloyl) thiazoles
Authors: Liu Yuyang , Yi Yang-Jie , Ye Jiao , Hu Ai-Xi 
Published: December 2022

Abstract
A series of 4-methyl-5-(3-phenylacryloyl)thiazoles based on chalcones were designed, synthesized and evaluated for their inﬂuenza neuraminidase (NA) inhibitory activity in vitro. A preliminary structure–activity relationship (SAR) analysis showed that thiazoles bearing amide had greater potency. It also showed that mono-hydroxyl group at 4-position on phenyl ring was more effective than other electron-releasing groups or electron-withdraw groups. Compounds A2 and A26 were more potent against NA with IC50 values of 8.2±0.5 μg/mL and 6.2±1.4 μg/mL, respectively. Molecular docking study demonstrated that thiazoles skeleton was benefit for the NA inhibitory activity.
Preparation of Polypropylene-grafted Graphene and its application on Polypropylene/Graphene Nanocomposites
Authors: Zhang He-xin , Yan Xin , Han Wei , Liu Chen , Chen Yujian , Xia You-yi , Cai Zheng-guo , Yoon Keun-Byoung , Yang Jian-ming 
Published: December 2022

Abstract
In the past few years, graphene-based nanocomposites are a hot
topic in the fields of materials and chemistry owing to their
unique features. We describe a facile melt blending method for
preparing polypropylene/graphene nanocomposites in this article.
The first stage involved grafting graphene onto chlorinated PP
using the Friedel-Crafts reaction. The second stage involved the
use of the resulting PP grafted graphene (PPG) as fillers in a melt
blend with commercial PP. After being grafted with an organic PP
chain, graphene becomes lipophilic; consequently, the PPG can be
dispersed well within the PP matrix while retaining excellent
interface adhesion. As a result, the thermostability, mechanical
performance, and electrical conductivity of PP were significantly
improved through the addition of PPG fillers. Additionally, with 10
wt% of PPG fillers (equivalent to 5 wt% graphenes), the
conductivity approaches 6.8 Sm-1. As a result of this
research, a new method for producing high-performance PP has been
developed.
Effect of time to minimally invasive esophagectomy after neoadjuvant chemotherapy for esophageal squamous cell carcinoma
Authors: Loc Nguyen Vo Vinh , Vuong Nguyen Lam , Trung Lam Viet , Trung Tran Thien 
Published: December 2022

Abstract
Background: Neoadjuvant chemotherapy (NAC) with docetaxel, cisplatin, and 5-fluorouracil/capecitabine (DCF/DCX) followed by esophagectomy has been the recommended treatment for esophageal squamous cell carcinoma (ESCC). However, the optimal interval from NAC to surgery has not yet been established. This study evaluated the impact of time to surgery (TTS) in the treatment of ESCC.
Methods: Between August 2018 and September 2021, 97 patients who underwent radical esophagectomy following 3-6 cycles of NAC with DCF/DCX for ESCC at a single hospital were analyzed. TTS was categorized into three groups: 16-41 days (group 1; 33 patients), 42-55 days (group 2; 29 patients), and 56-135 days (group 3; 35 patients). Survival outcomes included overall survival (OS) and progression-free survival (PFS).
Results:Mean age was 59.6±6.8 years, and 95 patients were male. One patient had grade-3 anemia, 12 had grade-2 anemia, and four had grade-2 neutropenia; all other NAC-related toxicities were as grade 1. Regarding pathologic tumor response, 18.6% achieved complete response, 71.1% achieved partial response, and 10.3% had stable disease. Forty-eight patients (49.5%) had a postoperative complication, but only six (6.2%) with grade-IIIa and two (2.1%) with grade IV-a according to the Clavien-Dindo classification. Median follow-up time was 24 months. Group 1 and 3 had worse OS (HR [95% CI]: 3.36 [1.16-11.7] and 1.83 [0.55-6.10]) and worse PFS (HR [95% CI]: 3.27 [1.25-8.53] and 1.61 [0.58-4.45]) compared to group 2.
Conclusion: The optimal TTS after NAC could be 6-8 weeks. The surgery should not be performed too early.
Reorientation Methodology for Reproducible Head Posture in Serial Cone Beam Computed Tomography Images
Authors: Mangal Utkarsh , Lee Sung Min , Lee Seeyoon , Cha Jung-Yul , Lee Kee-Joon , Yu Hyung-Seog , Jung Hong , Choi Sung-Hwan 
Published: December 2022

Abstract
Low dose and accessibility have increased the application of cone beam computed tomography (CBCT). Often serial images are captured for patients to diagnose and plan treatment in the craniofacial region. However, CBCT images are highly variable and lack harmonious reproduction, especially in the head's orientation. Though user-defined orientation methods have been suggested, the reproducibility remains controversial. Here, we propose a landmark-free reorientation methodology based on principal component analysis (PCA) for harmonious orientation of serially captured CBCTs. We analyzed three serial CBCT scans collected for 29 individuals who underwent orthognathic surgery. We first defined a region of interest with the proposed protocol by combining 2D rendering and 3D convex hull method, and identified an intermediary arrangement point. PCA identified the y-axis (anterioposterior) followed by the secondary x-axis (transverse). Finally, by defining the perpendicular z-axis, a new global orientation was assigned. The goodness of alignment (Hausdorff distance) showed a marked improvement (> 50%). Furthermore, we clustered cases based on clinical asymmetry and validated that the protocol was unaffected by the severity of the skeletal deformity. Therefore, it could be suggested that integrating the proposed algorithm as the preliminary step in CBCT evaluation will address a fundamental step towards harmonizing the craniofacial imaging records.
H2O2-mediated SRPED1 Inactivation Contributes to Resisting Ischemia-reperfusion Induced Oxidative Injury to Microglia
Authors: Wei Li , Li Xin , Wei Qianfeng , Chen Lin , Xu Li , Zhou Peng 
Published: December 2022

Abstract
Oxidative stress induced by ischemia-reperfusion is an important reason for the loss of neurons in the brain. In addition to direct oxidative toxicity that causes apoptosis of neurons and glial cells, oxidative stress can also cause complex physiological effects of related cells, such as activation of microglia to promote their repair effects on damaged areas. Here, we found that SPRED1, sprouty-related protein with an EVH1 domain 1, was significantly inhibited after ischemia-reperfusion by MCAO in rats, while the inhibition of SPRED1 responded to the up-expressed p65 proteins with the stimulation of H2O2. What’s more, we further revealed that SPRED1 tended to exert a unique role in maintaining intracellular homeostasis, which can re-activate the instinct of microglia upon the induction of H2O2 to resist oxidative toxicity. However, SPRED1 did not show such ability when it is only over-expressed but without H2O2 induction. In a word, these data revealed a potential role of SPRED1 in preventing cerebral ischemia-induced oxidative stress injury to nerve cells.
Microbe-responsive proteomes during plant-microbe interactions between rice genotypes and the multifunctional Methylobacterium oryzae CBMB20
Authors: Walitang Denver I. , Choudhury Aritra Roy , Parthiban Subramanian , Lee Yi , Jeong Bowon , Choi Geon , Cho Kun , Yun Sung Ho , Jamal Aysha Rizwana , Sa Tongmin 
Published: December 2022

Abstract
Background
Rice is colonized by plant growth promoting bacteria (PGPB) such as Methylobacterium leading to mutually beneficial plant-microbe interactions. As modulators of rice developmental process, Methylobacterium influences seed germination, growth, health, and development. However, little is known on the complex molecular responsive mechanisms modulating microbe-driven rice development. The application of proteomics to rice-microbe interactions helps us elucidate dynamic proteomic responses mediating this association.
Results
In this study, a total of 3908 proteins were detected across all treatments of which the non-inoculated IR29 and FL478 share up to 88% similar proteins. However, intrinsic differences appear in IR29 and FL478 as evident to the differentially abundant proteins (DAPs) and their associated gene ontology terms (GO). Successful colonization of M. oryzae CBMB20 in rice resulted to dynamic shifts in proteomes of both IR29 and FL478. The GO terms of DAPs for biological process in IR29 shifts in abundance from response to stimulus, cellular amino acid metabolic process, regulation of biological process and translation to cofactor metabolic process (6.31%), translation (5.41%) and photosynthesis (5.41%). FL478 showed a different shift from translation-related to response to stimulus (9%) and organic acid metabolic acid (8%). Both rice genotypes also showed a diversification of GO terms due to inoculation of M. oryzae CBMB20. Specific proteins such as peptidyl-prolyl cis-trans isomerase (A2WJU9), thiamine thiazole synthase (A2YM28), and alanine—tRNA ligase (B8B4H5) upregulated in IR29 and FL478 indicate key mechanisms of M. oryzae CBMB20 mediated plant growth promotion in rice.
Conclusions
Interaction of Methylobacterium oryzae CBMB20 to rice results to a dynamic, similar, and plant genotype-specific proteomic changes supporting associated growth and development. The multifaceted CBMB20 expands the gene ontology terms and increases the abundance of proteins associated to photosynthesis, diverse metabolic processes, protein synthesis and cell differentiation and fate potentially attributed to the growth and development of the host plant. The specific proteins and their functional relevance help us understand how CBMB20 mediate growth and development in their host under normal conditions and potentially link subsequent responses when the host plants are exposed to biotic and abiotic stresses.
Nomogram as a predictive model for depression risk in chronic obstructive pulmonary disease
Authors: Du Dan , Zhang XianMing , Ding ChaoWei , Yuan YaDong 
Published: December 2022

Abstract
Background
Depression after chronic obstructive pulmonary disease（COPD）is associated with mortality rates and poor prognosis. This study aimed to develop a nomogram to identify the risk of depression in patients with COPD based on predictors.
Methods
The Cross sectional study included 494 COPD aged >20 years who were come from the 2005–2008 National Health and Nutrition Examination Survey database. The 345 subjects from the 2005–2008 survey comprised the development group, and the remaining 149 subjects comprised the validation group. The least absolute shrinkage and selection operator (LASSO) binomial regression model was used to select the best predictive variables before further screening of multivariate regression model.The performance of the nomogram was evaluated on the basis of receiver operating characteristic curve(ROC), calibration curve, and clinical decision curve analysis (DCA).
Results
We reach a decision that there are 10 item,including
BMI,Race,Sex,Age,Education,marriage,hypertension,diabetes,CRP,MONO by LASSO regression model.Multivariate regression had selected 4 statistically significant variables for inclusion.as follow:Hypertension,MONO,CRP,Age.hypertension(Odds
Ratio[OR],0.836;95%confidence interval [CI],0.206-0.914; P = 0.028),MONO (OR, -2.652; 95% CI, 0.011 to 0.437; P=0.004), CRP (OR,0.238; 95% CI, 1.047 to 1.538; P=0.015) and Age (OR,0.031; 95% CI, 0.947 to 0.992; P=0.009).The AUC area under the curve for the training group was 0.774 whereas the validation group was 0.713, The predictive model was calibrated, and the DCA showed that the proposed nomogram had strong clinical applicability.
Conclusion
We have developed a simple nomogram to predict depression in COPD individuals based on Nomogram. External validation is needed to further demonstrate its predictive ability in primary care settings.
Synthesis of Some New 4-methylpiperazin-1-yl-methyl-4H-1,2,4-triazole-3- thiol Derivatives of Expected Anticancer and Antimicrobial Activity
Authors: CEBECİ YILDIZ UYGUN , Karaoğlu Sengül Alpay , Altun Muhammed 
Published: December 2022

Abstract
Different series of annulated methylpiperazine derivatives were designed, synthesized traditional methods, ana structurally characterized. 1,2,4-Triazole-fluoroquinolone and 1,2,4-triazole–conazole hybrids are designed, synthesized, and investigated in vitro against a variety of common diseases. The structure of the newly synthesized compounds are characterized from spectral data (IR, 1H NMR, 13C NMR, and LC–MS). The antibacterial activity against both Gram-positive and Gram-negative bacteria is shown to be enhanced by many of the produced compounds. Also, some of the products are found to have strong antiproliferative effects against HeLa cervical cancer cells, whilst demonstrating cytotoxic effects toward normal cells. Compounds 6d and 6e showed intermediate anticancer activity.