bluuebunny/crossref_metadata_2025_split huggingface dataset:
14-channel neurofeedback with Auto Train Brain improves the left lateralization of the brain in dyslexia: A pilot study
Authors: Eroglu Gunet 
Published: October 2022

Auto Train Brain is a neurofeedback-based mobile application that increases reading comprehension and reading speed in dyslexia with EMOTIV EPOC-X which has 14 channels. The clinical trials have been completed on dyslexia beforehand. The left hemisphere-related deficits are known in dyslexia. In this research, we have investigated the positive long-term effects of Auto Train Brain to improve the cognitive abilities of dyslexic people based on the measurements of the variance of gamma band sample entropy across neurofeedback sessions. The previous research indicates that the increase in the variance of the gamma band entropy shows the increased adaptations in the functional networks. 14-channel neurofeedback with Auto Train Brain increases the variance of gamma band entropy in the left temporal lobe (T7) over the right temporal lobe (T8) which may be translated as the adaptations of the functional networks in the left temporal region are increased after 100 sessions of neurofeedback in terms of electrophysiology.
PREPARATION OF INFILTRATION WELL DEVELOPMENT PLANNING IN KADIRI UNIVERSITY AREA
Authors: Susanto Sony , Ahmad Ridwan  , Sigit Winarno  , Yosef Cahyo Setianto  
Published: October 2022

Along with the development in Indonesia, resulting in the reduction of vacant land used as water infiltrated into the ground. This causes frequent flooding such as at Kadiri University, Kediri City there are often flood puddles when rainfall is high. To overcome this disaster, it is necessary to create infiltration wells in the area because the infiltration wells are so effective, and practical. This study aims to overcome or overcome puddles at Kadiri University. The metodologi penelitian is carried out in several stages, namely the primary and secondary data collection stage, the second processing of parametric testdistributiondata, the third calculation of water discharge and smirnov kolmogrov test, thefourth determination of the dimensions of infiltration wells. The final result of the analysis obtained sage of infiltration in the form of a circle with a diameter of 1.4 meters depth of 3 meters a number of 153 pieces. Dengan the existence of infiltration wells later is expected to reduce puddles in the campus area.
Pengembangan Modul Berbasis Kvisoft Flipbook Maker Materi Pendudukan Jepang untuk Mata Pelajaran Sejarah Kelas XI SMAN 3 Probolinggo
Authors: Wibowo Pujianto Hari , Leksono Ibut Priono , Rohman Ujang 
Published: October 2022

Pandemi Covid-19 jugamenuntut sekolah dan guru untuk melaksanaan pembelajaran secara online karena terkait dengan kebijakan pemerintah untuk melaksanakan Pembelajaran Tatap Muka Terbatas (PTMT). Guru dituntut untuk dapat melaksanakan pembelajaran dengan memanfatkan dan memaksimalkan perkembangan Teknologi Informatika (TI). Hal ini tentu menjadi tantangan bagi guru untuk dapat memfasilitasi siswa dengan memberikan sumber belajar yang efisien, sehingga siswa dapat belajar secara mandiri. Bahan ajar yang digunakan disekolah kurang menarik peserta didik dalam pembelajaran sejarah karena kurang berkaitan dengan kemajuan IPTEK masa kini. Tujuan yang ingin dicapai dalam penelitian ini adalah menghasilkan produk berupa modul interaktif yang sudah tervalidasi oleh ahli, yaitu ahli materi, media, dan desain. Produk ini diujicobakan pada kelompok kecil dengan responden 9 siswa dan kelompok besar dengan jumlah responden 35 siswa. Pengembangan modul interaktif ini menggunakan model pengembangan Dick and Carey yang terdapat 10 tahapan. Modul interaktif berbasis Kvisoft Flipbook Maker diharapkan dapat menciptakan pembelajaran mandiri dan dapat memberikan pengalaman yang berkesan dan bermakna.
The Pengaruh Pengaruh Kualitas Pelayanan Petugas Pajak, Sanksi Perpajakan, Tarif Pajak Dan Kondisi Keuangan Terhadap Kepatuhan Wajib Umkm Selama Masa Pandemi Covid-19
Authors: Irawan Rifka Ayu , Putri Anisa 
Published: October 2022

Penelitian ini bertujuan untuk mengetahui pengaruh kualitas pelayanan petugas pajak, sanksi perpajaka, tarif pajak dan kondisi keuangan terhadap kepatuhan wajib pajak UMKM selama masa pandemi covid-19. Metode penelitian menggunakan metode kuantitatif. Penelitian dilakukan kepada UMKM di Kota Bekasi dengan 23.000 populasi tahun 2021. Sampel penelitian yang digunakan adalah 100 responden pengusaha UMKM di Kota Bekasi. Dengan teknik pangambilan sampel menggunakan purposive sampling. Metode analisis regresi yang digunakan adalah regresi linear berganda. Hasil uji statistik, variabel kualitas pelayanan petugas pajak, sanksi perpajakan dan kondisi keuangan berpengaruh positif dan signifikan terhadap kepatuhan wajib pajak UMKM di Kota Bekasi. Sedangkan tarif pajak tidak berpengaruh terhadap kepatuhan wajib pajak UMKM di Kota Bekasi.
Kappa-carrageenan-Functionalization of octacalcium phosphate-coated titanium Discs enhances pre-osteoblast behavior and osteogenic differentiation
Authors: Cao Wei , Jin Jianfeng , Wu Gang , Bravenboer Nathalie , Helder Marco N. , Schulten Engelbert A. J. M. , Bacabac Rommel G. , Pathak Janak L. , Klein-Nulend Jenneke 
Published: October 2022

Bioactive coatings are promising for improving osseointegration and the long-term success of titanium dental or orthopaedic implants. Biomimetic octacalcium phosphate (OCP) coating can be used as a carrier for osteoinductive agents. κ-Carrageenan, a highly hydrophilic and biocompatible seaweed-derived sulfated-polysaccharide, promotes pre-osteoblast activity required for bone regeneration. Whether κ-carrageenan can functionalize OCP-coating to enhance osseointegration of titanium implants is unclear. This study aimed to analyze carrageenan-functionalized biomimetic OCP-coated titanium structure, and effects of carrageenan functionalization on pre-osteoblast behavior and osteogenic differentiation. Titanium discs were coated with OCP/κ-carrageenan at 0.125–2 mg/ml OCP solution, and physicochemical and biological properties were investigated. κ-Carrageenan (2 mg/ml) in the OCP coating of titanium discs decreased the pore size in the sheet-like OCP crystal by 41.32%. None of the κ-carrageenan concentrations tested in the OCP-coating did affect hydrophilicity. However, κ-carrageenan (2 mg/ml) increased (1.26-fold) MC3T3-E1 pre-osteoblast spreading at 1 h i.e., κ-Carrageenan in the OCP-coating increased pre-osteoblast proliferation (max. 1.92-fold at 2 mg/ml, day 1), metabolic activity (max. 1.50-fold at 2 mg/ml, day 3), and alkaline phosphatase protein (max. 4.21-fold at 2 mg/ml, day 3), as well as matrix mineralization (max. 5.45-fold at 2 mg/ml, day 21). κ-Carrageenan (2 mg/ml) in the OCP-coating increased gene expression of Mepe (4.93-fold) at day 14, and Runx2 (2.94-fold), Opn (3.59-fold), Fgf2 (3.47-fold), Ocn (3.88-fold), and Dmp1 (4.59-fold) at day 21 in pre-osteoblasts. In conclusion, κ-carrageenan modified the morphology and microstructure of OCP-coating on titanium discs, and enhanced pre-osteoblast metabolic activity, proliferation, and osteogenic differentiation. This suggests that κ-carrageenan-functionalized OCP coating may be promising for in vivo improvement of titanium implant osseointegration.
Ciliated epithelia are key elements in the recruitment of bacterial partners in the squid-vibrio symbiosis
Authors: Gundlach Katrina A. , Nawroth Janna , Kanso Eva , Nasrin Farzana , Ruby Edward G. , McFall-Ngai Margaret 
Published: October 2022

The Hawaiian bobtail squid, Euprymna scolopes, harvests its luminous symbiont, Vibrio fischeri, from the surrounding seawater within hours of hatching. During embryogenesis, the host animal develops a nascent light organ with ciliated fields on each lateral surface. We hypothesized that these fields function to increase the efficiency of symbiont colonization of host tissues. Within minutes of hatching from the egg, the host’s ciliated fields shed copious amounts of mucus in a non-specific response to bacterial surface molecules, specifically peptidoglycan (PGN), from the bacterioplankton in the surrounding seawater. Experimental manipulation of the system provided evidence that nitric oxide in the mucus drives an increase in ciliary beat frequency (CBF), and exposure to even small numbers of V. fischeri cells for short periods resulted in an additional increase in CBF. These results indicate that the light-organ ciliated fields respond specifically, sensitively, and rapidly, to the presence of nonspecific PGN as well as symbiont cells in the ambient seawater. Notably, the study provides the first evidence that this induction of an increase in CBF occurs as part of a thus far undiscovered initial phase in colonization of the squid host by its symbiont, i.e., host recognition of V. fischeri cues in the environment within minutes. Using a biophysics-based mathematical analysis, we showed that this rapid induction of increased CBF, while accelerating bacterial advection, is unlikely to be signaled by V. fischeri cells interacting directly with the organ surface. These overall changes in CBF were shown to significantly impact the efficiency of V. fischeri colonization of the host organ. Further, once V. fischeri has fully colonized the host tissues, i.e., about 12–24 h after initial host-symbiont interactions, the symbionts drove an attenuation of mucus shedding from the ciliated fields, concomitant with an attenuation of the CBF. Taken together, these findings offer a window into the very first interactions of ciliated surfaces with their coevolved microbial partners.
Preparation of rambutan-like Co0.5Ni0.5Fe2O4 as anode for high–performance lithium–ion batteries
Authors: Wang Qian , Wu Yongzi , Pan Ning , Yang Chenyu , Wu Shuo , Li Dejie , Gu Shaonan , Zhou Guowei , Chai Jinling 
Published: October 2022

NiFe2O4 is a kind of promising lithium ion battery (LIB) electrode material, but its commercial applications have been limited due to the electronic insulation property and large volume expansion during the conversion reaction process, which results in rapid capacity decrease and poor cycling stability. We synthesized rambutan-like Co0.5Ni0.5Fe2O4 using the self-templating solvothermal method. The special structure of Co0.5Ni0.5Fe2O4 which was formed by the assembly of numerous nanosheets could effectively buffer the volume change during the charging and discharging process. Partial substitution of Ni with Co. in NiFe2O4 leads to Co0.5Ni0.5Fe2O4, the coexisting of both nickel and cobalt components is expected to provide more abundant redox reactions. The specific capacity of the rambutan-like Co0.5Ni0.5Fe2O4 as an anode material for LIB could reach 963 mA h g−1 at the current density of 500 mA g−1 after 200 cycles, confirming that the as-synthesized material is a promising candidate for LIBs.
Transcatheter mitral valve replacement in native mitral valve with severe mitral annular calcification: Skirting the Sapien 3 to reduce the risk of paravalvular leaks
Authors: Pozzoli Alberto , Torre Tiziano , Pedrazzini Giovanni , Demertzis Stefanos , Ferrari Enrico 
Published: October 2022

BackgroundMitral annular calcification (MAC) may represent a significant challenge for heart surgeons with an extremely high perioperative risk during mitral valve (MV) surgery. The risk is further increased when patients fail to be eligible for any percutaneous treatment, particularly because circumferential calcifications involving the anterior leaflet suggest a critical obstruction of the left ventricular outflow tract (LVOT).ObjectivesThe objective of this study was to evaluate residual mitral regurgitation (MR) after surgical mitral valve replacement using a Sapien 3 Ultra (Edwards Lifesciences, CA, USA) transcatheter aortic valve implantation (TAVI) prosthesis, reinforced with a pericardial skirt, in high-risk selected patients with severe MAC.MethodsSince 2020, five high-risk patients (mean age 70 years; 63–76; four women) with severe mitral disease in the context of severe MAC (computed tomography-based mean MAC Score 8.2 ± 1.1) were operated on after we adopted this novel technique. The operations were performed under general anesthesia, using a transapical TAVI delivery system to position the Sapien 3 in the mitral position under direct vision. To reinforce and avoid paravalvular leakages, a pericardial skirt was previously sewn around the prosthesis, securing it to the annulus and perivalvular atrial surface.ResultsSapien 3 Ultra implantation was successful without residual MR in all five patients (mild paravalvular leak in one case). Four patients had a 29-mm valve implanted, while one had a 26-mm valve implanted. Predilatation of the native annulus was never performed. Perfusion and clamping times were 134 ± 53 mins and 108 ± 43 mins, respectively. The presence of the pericardial skirt reduced the risk of leakage between the prosthesis and the rigid calcium surface, with final mean and maximal gradients of the TAVI prosthesis of 4.1 and 10.8 mmHg, respectively. There were no left ventricular outflow tract obstructions (mean LVOT gradient of 8 ± 1 mmHg). All patients were discharged, and neither mortality nor prosthetic dysfunction, nor residual mitral regurgitation was recorded. During follow-up, the last patient treated (MAC Score 10, severe calcification of the mitro-aortic junction) returned to our attention with a significant recurrent jet originating from the anterolateral commissure, currently medically treated, given the prohibitive redo risk.ConclusionDirect open surgical implantation of the Sapien 3 valve can be safely done in patients with severe MAC in dedicated centers. Reinforcing the TAVI prosthesis by sewing a pericardial skirt led to satisfactory perioperative and early postoperative results, reducing paravalvular leakages. Complex anatomies have a CERTAIN risk of recurrence.
From drugs to targets: Reverse engineering the virtual screening process on a proteomic scale
Authors: Schottlender Gustavo , Prieto Juan Manuel , Palumbo Miranda Clara , Castello Florencia A. , Serral Federico , Sosa Ezequiel J. , Turjanski Adrián G. , Martì Marcelo A. , Fernández Do Porto Darío 
Published: October 2022

Phenotypic screening is a powerful technique that allowed the discovery of antimicrobials to fight infectious diseases considered deadly less than a century ago. In high throughput phenotypic screening assays, thousands of compounds are tested for their capacity to inhibit microbial growth in-vitro. After an active compound is found, identifying the molecular target is the next step. Knowing the specific target is key for understanding its mechanism of action, and essential for future drug development. Moreover, this knowledge allows drug developers to design new generations of drugs with increased efficacy and reduced side effects. However, target identification for a known active compound is usually a very difficult task. In the present work, we present a powerful reverse virtual screening strategy, that can help researchers working in the drug discovery field, to predict a set of putative targets for a compound known to exhibit antimicrobial effects. The strategy combines chemical similarity methods, with target prioritization based on essentiality data, and molecular-docking. These steps can be tailored according to the researchers’ needs and pathogen’s available information. Our results show that using only the chemical similarity approach, this method is capable of retrieving potential targets for half of tested compounds. The results show that even for a low chemical similarity threshold whenever domains are retrieved, the correct domain is among those retrieved in more than 80% of the queries. Prioritizing targets by an essentiality criteria allows us to further reduce, up to 3–4 times, the number of putative targets. Lastly, docking is able to identify the correct domain ranked in the top two in about two thirds of cases. Bias docking improves predictive capacity only slightly in this scenario. We expect to integrate the presented strategy in the context of Target Pathogen database to make it available for the wide community of researchers working in antimicrobials discovery.
Year-long monitoring of phytoplankton community, toxigenic cyanobacteria, and total microcystins in a eutrophic tropical dam supplying the Mexico megacity
Authors: Martínez-Jerónimo Fernando , Antuna-González Paloma del Carmen , Hernández-Zamora Miriam , Martínez-Jerónimo Laura , Munoz Gabriel , Simon Dana F. , Sauvé Sébastien 
Published: October 2022

The Valle de Bravo dam is an important source of drinking water supply for the Mexico City Conurbation (&gt;26 million inhabitants) and is also a hotspot for nautical sports, recreational fishing, and tourism. However, anthropogenic pressures in the upper reaches of the basin have led to increased eutrophication and numerous harmful cyanobacterial blooms (HCBs). To determine the effect of abiotic variables on the characteristics of the phytoplanktonic community, as well as their influence on the diversity of toxigenic cyanobacteria and related cyanotoxins, we evaluated monthly variations in environmental factors, phytoplankton biovolume and composition and total microcystins in Valle de Bravo dam during the year of 2019. Overall, 75 phytoplanktonic species were identified, mostly belonging to the divisions Cyanobacteria, Chlorophyta, and Bacillariophyta. Cyanobacteria presented the highest biomass throughout the year, with water temperature and rainfall being the best correlated factors. The highest concentrations of total microcystins were recorded from July to September (maximum of 71 μg L−1), associated with the dominance of species such as M. smithii, M. aeruginosa, M. viridis M. flos-aquae, Aphanocapsa planctonica, and Dolichospermum crassum, and environmental factors such as rainfall, total dissolved solids, water temperature, and specific trace metals. In all months except December, the total cyanobacterial biovolume was above the World Health Organization alert level two, indicating potentially high risks to human health. The predominance of cyanobacteria and high biovolumes classified water quality as poor to very poor during most of the year. To avoid the development of HCBs in this dam, the contribution of nutrients by the tributary rivers and sewage must be controlled, since the poor-quality conditions of the water threaten the aquatic biota, the sports and recreation activities and affect the potable water supply.
Establishment and validation of a polygene prognostic model for clear cell renal cell carcinoma
Authors: Gan Kai , Zhang Keying , Li Yu , Zhao Xiaolong , Li Hongji , Xu Chao , Liu Shaojie , Zhang Chao , Han Donghui , Wen Weihong , Qin Weijun 
Published: October 2022

Purpose: To establish an effective prognostic model for patients with clear cell renal cell carcinoma (ccRCC).Methods: We identified four hub differentially expressed genes (DEGs) in Gene Expression Omnibus (GEO) database and verified them in the Cancer Gene Atlas (TCGA), STRING, UALCAN, TIMER, and Gene Expression Profiling Interactive Analysis (GEPIA) databases. We then used TCGA and International Cancer Genome Consortium (ICGC) to identify tumor pathway molecules highly correlated with hub DEGs. And by further LASSO and Cox regression analysis, we successfully identified five genes as prognostic factors.Results: We successfully identified a risk prediction model consisting of five genes: IGF2BP3, CDKN1A, GSDMB, FABP5, RBMX. We next distributed patients into low-risk and high-risk groups using the median as a cutoff. The low-risk group obviously had better survival than those in the predicted high-risk group. The results showed discrepancies in tumor-associated immune cell infiltration between risk groups. We also combined the risk model with clinical variables to create a nomogram.Conclusion: Our model has a satisfactory predictive effect on the prognosis of ccRCC patients and may provide new ideas for future immune therapy.
Prognostic value of hematologic parameters in advanced non-small cell lung cancer patients receiving anti-PD-1 inhibitors
Authors: Zhao Xinmin , Wu Xianghua , Yu Hui , Wang Huijie , Sun Si , Hu Zhihuang , Liu Cuicui , Zhang Junli , Shao Yang , Wang Jialei 
Published: October 2022

BackgroundThe association between hematologic parameters and anti-programmed death-1 (PD-1) inhibitors was generally examined without considering therapy lines and medicine types. The study was aimed to identify potential hematologic biomarkers associated with clinical outcome in patients with non-small cell lung cancer (NSCLC) treated with first-line pembrolizumab and subsequent-line nivolumab.Materials and methods161 NSCLC patients were categorized into first-line pembrolizumab group (pembrolizumab group) and subsequent-line nivolumab group (nivolumab group). Univariate and multivariate Cox regression analyses were used to evaluate the prognostic value of hematologic parameters for clinical outcomes.ResultsThe median progression-free survival (mPFS) was 9.6 months in the pembrolizumab group and 4.1 months in the nivolumab group (HR =1.61; P = 0.012); the median overall survival (mOS) was not reached in the pembrolizumab group and 17.7 months in the nivolumab group (HR =1.37; P = 0.23). Of the 79 patients in the pembrolizumab group, baseline PD-L1 tumor proportion score (TPS)≥1% was an independent factor of longer PFS and OS. Age≥60 years, absolute platelet count (APC)≥220×109/L and platelet-to-lymphocyte ratio (PLR)≥120 were associated with inferior PFS. Of the 82 patients in the nivolumab group, absolute neutrophil count (ANC)≥3×109/L was associated with longer PFS, while LDH (lactate dehydrogenase)≥160 U/L was associated with inferior PFS and derived neutrophil-to-lymphocyte ratio (dNLR)≥1.2 was associated with longer OS.ConclusionOur study identified multiple clinically accessible prognostic biomarkers in the peripheral blood in both the pembrolizumab and nivolumab subgroups.
Immune cells transcriptome-based drug repositioning for multiple sclerosis
Authors: Yin Xinyue , Rang Xinming , Hong Xiangxiang , Zhou Yinglian , Xu Chaohan , Fu Jin 
Published: October 2022

ObjectiveFinding target genes and target pathways of existing drugs for drug repositioning in multiple sclerosis (MS) based on transcriptomic changes in MS immune cells.Materials and MethodsBased on transcriptome data from Gene Expression Omnibus (GEO) database, differentially expressed genes (DEGs) in MS patients without treatment were identified by bioinformatics analysis according to the type of immune cells, as well as DEGs in MS patients before and after drug administration. Hub target genes of the drug for MS were analyzed by constructing the protein-protein interaction network, and candidate drugs targeting 2 or more hub target genes were obtained through the connectivity map (CMap) database and Drugbank database. Then, the enriched pathways of MS patients without treatment and the enriched pathways of MS patients before and after drug administration were intersected to obtain the target pathways of the drug for MS, and the candidate drugs targeting 2 or more target pathways were obtained through Kyoto Encyclopedia of Genes and Genomes (KEGG) database.ResultsWe obtained 50 hub target genes for CD4+ T cells in Fingolimod for MS, 15 hub target genes for Plasmacytoid dendritic cells (pDCs) and 7 hub target genes for Peripheral blood mononuclear cells (PBMC) in interferon-β (IFN-β) for MS. 6 candidate drugs targeting two or more hub targets (Fostamatinib, Copper, Artenimol, Phenethyl isothiocyanate, Aspirin and Zinc) were obtained. In addition, we obtained 4 target pathways for CD19+ B cells and 15 target pathways for CD4+ T cells in Fingolimod for MS, 7 target pathways for pDCs and 6 target pathways for PBMC in IFN-β for MS, most of which belong to the immune system and viral infectious disease pathways. We obtained 69 candidate drugs targeting two target pathways.ConclusionWe found that applying candidate drugs that target both the “PI3K-Akt signaling pathway” and “Chemokine signaling pathway” (e.g., Nemiralisib and Umbralisib) or applying tyrosine kinase inhibitors (e.g., Fostamatinib) may be potential therapies for the treatment of MS.