bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation changes of microbial communities and metabolites in tobacco waste extract during its fermentation with additional Zygosaccharomyces sp. MC-5K3
Authors: Shu Ming , Yang Yang , Pan Fanda , Bian Tengfei , Yuan Kailong , Liao Fu , He Wenmiao , Li Shitou , Xu Jian , Jiao Yang , Hu Tong , Zhong Weihong 
Published: September 2022

AbstractTobacco (Nicotiana tabacumL.) is a very important economic crop in China but lead to a large quantity of tobacco waste during processing. Nowadays, industrial tobacco waste was mainly treated via reconstituted tobacco process by papermaking method, during which liquid tobacco waste extract (TWE) is the key fermentation step to control the final product quality. As bioaugmentation with specific functional microorganisms was one effective method in the quality optimization of fermented products, some native strains were isolated from TWE and used as bioaugmentation agents. In this study,Zygosaccharomycessp. MC-5K3 isolated from TWE was used as the additive to improve the quality of fermented TWE. The results proved that the addition ofZygosaccharomycessp. MC-5K3 significantly influence the microbial diversity of fermented TWE especially for the fungal groups thatZygosaccharomycesbecome the only dominant fungal genus instead of some pathogenic bacterial genera. Then the metabolomics profiling of fermented TWE showed that the variation in the concentration of 3-Hydroxybenzoic acid was the key influence factor on fermented TWE quality after MC-5K3 was added. The correlation analysis showed that the change of microbial diversity of the fermented production due to the addition of MC-5K3 leaded to some important differential metabolites such as 3-Hydroxybenzoic acid and finally improved various qualities of tobacco products. In conclusion, this study lays a theoretical foundation and new microbiology methods for improving the quality of tobacco products by new microbiology methods.
Oral bioavailability of microdoses and therapeutic doses of midazolam as a 2-dimensionally printed orodispersible film in healthy volunteers
Authors: Breithaupt Mareile H. , Krohmer Evelyn , Taylor Lenka , Koerner Eva , Hoppe-Tichy Torsten , Burhenne Juergen , Foerster Kathrin I. , Dachtler Markus , Huber Gerald , Venkatesh Rakesh , Eggenreich Karin , Czock David , Mikus Gerd , Blank Antje , Haefeli Walter E. 
Published: September 2022

Abstract
Purpose
Use of two-dimensional (2D) printing technologies of drugs on orodispersible films (ODF) can promote dose individualization and facilitate drug delivery in vulnerable patients including children. We investigated midazolam pharmacokinetics after administration of 2D-printed ODF (EudraCT 2020-003984-24).
Methods
Midazolam doses of 0.03 mg and 3 mg were printed on an ODF using a 2D drug printer. We investigated the bioavailability of the two midazolam doses with ODF swallowed immediately (ODF-IS) or delayed after 2 min (ODF-DS) by comparing their pharmacokinetics with intravenous and oral midazolam solution in 12 healthy volunteers.
Results
The relative bioavailability of ODF-IS 0.03 mg was 102% (90% confidence interval: 89.4–116) compared to oral solution and for 3 mg 101% (86.8–116). Cmax of ODF-IS 0.03 mg was 95.5% (83.2–110) compared to oral solution and 94.3% (78.2–114) after 3 mg. Absolute bioavailability of ODF-IS 0.03 mg was 24.9% (21.2–29.2) and for 3 mg 28.1% (23.4–33.8) (oral solution: 0.03 mg: 24.4% (22.0-27.1); 3 mg: 28.0%, (25.0-31.2)). Absolute bioavailability of ODF-DS was significantly larger than for ODF-IS (0.03 mg: 61.4%; 3 mg: 44.1%; both p < 0.0001).
Conclusion
This trial demonstrates the tolerability and unchanged bioavailability of midazolam printed on ODF over a 100-fold dose range, proving the suitability of ODF for dose individualization. Midazolam ODF-IS AUC0−∞ in both doses was bioequivalent to administration of an oral solution. However, Cmax of the therapeutic dose ODF-IS missed bioequivalence by a clinically not relevant extent. Prolonged mucosal exposure increased bioavailability.
Trajectories of freshwater microbial genomics and greenhouse gas saturation upon glacial retreat
Authors: Wei Jing , Fontaine Laurent , Valiente Nicolas , Dörsch Peter , Hessen Dag , Eiler Alexander 
Published: September 2022

Abstract
Due to climate warming, ice sheets around the world are losing mass, contributing to changes in runoff, loads of nutrients and organic carbon to recipient lakes and rivers, and on a longer time span to greening of terrestrial landscapes. These changes are expected to affect microbial communities and the release of greenhouse gases from these systems, and thus repercuss to climate. However, these repercussions are poorly constrained mostly due to limited knowledge on microbial responses to deglaciation. Using genomic and chemical data from freshwater chronosequences in Arctic Svalbard and Alpine Norway, we reveal the genomic succession from chemolithotrophic to photo- and heterotrophic microbial taxa upon glacial retreat and nutrient fertilization by birds. The highly resolved trait patterns were related to greenhouse gas concentrations including methane and carbon dioxide supersaturation. Although methanotrophs were present and increased along the chronosequence, methane consumption rates were low even in supersaturated systems. Nitrous oxide oversaturation and genomic information suggest active nitrogen cycling across the entire deglaciated landscape, and in the high Arctic, birds served as major modulators at many sites. Our findings show diverse microbial succession patterns, and trajectories in carbon and nitrogen cycle processes representing a positive feedback loop of deglaciation on climate warming.
Nutritional Habits and Lifestyle Changes of Nursing Students Before and During the Covid-19 Pandemic
Authors: Bayrak  , Oğuz Prof. Dr. , Sütcü Nurse , Şahin Nurse , Aydın Nurse 
Published: September 2022

Abstract
Aim
The research was intended to determine the nutritional habits and lifestyle change of nursing students before and during the pandemic of Covid-19.
Method
In the population of nursing department students at a public university in Istanbul, between January 2020 and December 2021 the sample is formed by 548 students who agreed to participate in the research and filled out the survey questions. Student Diagnosis Form and Nutritional Habits Index were used to collect the data.
Results
During the pandemic period, 71.2% of students eat three main meals, 56.9% have the habit of eating at night, 21.4% use vitamins, 40.5% do physical activity, 13% smoke, 7.7% drink alcohol and 76.5% have sleep disorders. Compared to the pre-pandemic period, it was observed that the risky eating behaviors of the students decreased during the pandemic and there were moderately risky eating behaviors (p = 0.000). In addition, students in the fourth grade (p = 0.015), non-smokers (p = 0.000), non-alcoholic (p = 0.030), those who did not gain weight during the pandemic (p = 0.015), those who did not skip meals (p = 0.002), and those who did not eat at night (p = 0.030). =0.000), those who doing exercise (p = 0.010), those who do not have sleep problems (p = 0.000) have less risky eating behaviors.
Conclusion
students' physical inactivity, night eating habits and sleep disorders have increased during the pandemic period. Those who do not smoke, do not drink alcohol, do not skip meals, do not gain weight, do not eat at night, exercise and have less risky eating behaviors during the pandemic period.
Analysis the anti-vaccination movement in Afghanistan
Authors: sadati kimia mohammadi and ahmad kalateh 
Published: September 2022

Abstract
Afghanistan is a country that, suffers with wars and problems related to health issues, and has many shortcomings. Especially in the area of infectious diseases such as measles, corona, paralysis, etc. There are many health challenges in remote areas since the spread of vaccines in this field in Afghanistan. Many groups opposed this process, including religious groups, rural women, clerics and other hidden groups that benefited from this. Therefore, this group talked about the harms of vaccination in mass media, gathered places and made people hesitate not to vaccinate, hence, this has become a challenge. The solution is to cooperate between the cooperating organizations and people who support the vaccine and increasing the level of public awareness of the vaccine, can go through a successful process in Afghanistan. Almost majority of Afghan people are Muslims, and this has played a constructive role in determining the success of vaccination groups, and most of the anti-vaccination movements arise among religious groups. The research conducted in 2017 by Ali Ahmed amid Islamic countries has reached the conclusion that in Islamic countries, parents value moral approvals to vaccinate their children and get fatwas from clerics to vaccinate them, and sometimes the clerics refuse to do it because of disorder. DNA is known and causes its lack of trust among people, which lays the foundation of anti-vaccination movements(Ali ahmad &etl, 201; 154P).
Spinal–pelvic sagittal imbalance and paraspinal muscle degeneration in patients with degenerative lumbar spinal stenosis
Authors: BAO TIANLIAN , WANG YONGJIANG , ZHANG QINGXIN , GAO FENG , LIU HAO , TAO XIAOYANG , GAO GANG , YANG WUPENG , ZHANG TINXIN , ZHAO KEYU 
Published: September 2022

Abstract
Background: To explore the relationship between paraspinal muscle degeneration and changes in spine-pelvic sagittal parameters in patients with degenerative lumbar spinal stenosis (DLSS).
Method: Twenty-eight patients with DLSS and complete imaging data from January 2020 to January 2022 were included. The relative cross-sectional area (RCSA) and fatty infiltration ratio (FIR) of the multifidus (MF) and erector spinae (ES) were obtained on lumbar magnetic resonance imaging. The sagittal vertical axis, thoracic kyphosis (TK), lumbar lordosis (LL), pelvic incidence, sacral slope and pelvic tilt were analysed by Spearman rank correlation analysis, and the relationship between paraspinal muscle parameters and spine–pelvic sagittal imbalance in patients with DLSS was determined. Correlation analysis of state parameters.
Result: In the DLSS (L4-5) group, the right ES FIR was negatively correlated with the TK angle (r = −0.536, P < 0.05) and the left side had no significance. The left MF RCSA was associated with the TK angle. In the DLSS (L5-S1) group, the right MF RCSA and right ES RCSA were significantly positively correlated with the TK angle (r = 0. 502, P < 0.05, r = 0. 685, r = 0. 615, P < 0.05), and the left side has no meaning.
Conclusion: A correlation was found between paraspinal muscle parameters and spine–pelvic sagittal parameters in patients with DLSS.
Folcare biostimulant application in pea (Pisum sativum) crops under ZnO bionanoparticle exposure
Authors: Missaoui Takwa , Chemingui Hajer , Elboughdiri Noureddine , Sobeai Sanad M.Al , smiri moez 
Published: September 2022

Abstract
The purpose of this study was to identify the most important physiological and biological effects of green synthesis ZnO nanoparticles at a size of 65 nm, biostimulant (Folcare) and interaction biostimulant ZnO NPs on plant growth and metabolism. As knowledge about biostimulants and their preventive and recovery modes of action has improved, it is important to keep the optimum result in terms of yield and quality of crops. The reduction of fertilisers has to be substituted by strategies that improve the nutrients uptake or their utilisation by the plants. New processing methods are required as an efficient green process or an integrated (hybrid) process for different new technologies of interest. The effects of NPs, biostimulant and combination ZnO NPs biostimulant on plant cells metabolism were examined in cytosol, chloroplast and mitochondria of cells from the setems, roots and leaves. The interaction NPs/biostimulant had a beneficial effect on the morphological and physiological indicators of plant health than when nanoparticles and biostimulant are applicated separately.Folcare biostimulant coupled with zinc oxide nanoparticles improved pea crops growth. The improved of the quality of pea plants can be explained at least, in part, by increase in antioxidant activities during plant growth phenophase.
A New Establishment of Cuproptosis-Related Long Non-Coding RNA Signature to Predict Prognosis in Head and Neck Squamous cell Carcinomas
Authors: wang junrong , gao weitong , Xue Yuting , wang xueying , Guo Lunhua , Yang Xianguang , Sun Ji , Miao Susheng , Mao Xionghui 
Published: September 2022

Abstract
Background
Cuproptosis is a novel type of programmed cell death which plays an important role in the development and progression of cancer. However, there is a limited amount of research on cuproptosis-associated long non-coding RNAs (lncRNAs) in head and neck squamous cell carcinomas (HNSCCs). This study aimed to investigate the predictive value of cuproptosis-related lncRNA signature for HNSCC prognosis.
Method
Transcriptomic and clinical data of HNSCC patients were obtained from the Cancer Genome Atlas (TCGA). We established a cuproptosis-related lncRNA signature and then constructed a hybrid nomogram based on risk scores and clinical factors. We also performed differential expression genes (DEGs) function, immune cells infiltration, immune checkpoint analysis based on cuproptosis-associated lncRNA signature.
Results
A signature of 27 cuproptosis-related lncRNAs was performed and the prognosis of patients at high risk is worse compared with patients at low risk based on above signature. A nomogram which integrated risk scores and clinical features also showed favorable predictive power. Furthermore, DEGs in high or low risk group were mainly enriched in immune-related pathways. Anti-tumor immune cells and immune checkpoints were mainly enriched in low risk group compared with high risk group.
Conclusion
Cuproptosis-related lncRNAs could be regarded as independent indicators for HNSCC prognosis which might be effective targets for HNSCC therapy.
Life Cycle Assessment of Guava Production and Distribution Systems
Authors: Imtiyaz Hena , Soni Peeyush , Vimolwan Yukongdi 
Published: September 2022

Abstract
The life cycle assessment of various processes and materials used during production phase of guava revealed that the production and application of agricultural inputs were the major contributors to global warming, fresh water aquatic ecotoxicity, terrestrial ecotoxicity, acidification and eutrophication as well as caused highest damage to the ecosystem. The application of zinc monosulphate as micronutrient had major impact on abiotic depletion, ozone layer depletion, human toxicity and photochemical oxidation as well as caused highest damage to human health and resources depletion. The life cycle assessment during distribution phase revealed that production and consumption of polyvinyl chloride crates for packaging of guava was a major contributor to abiotic depletion, global warming, human toxicity and eutrophication, whereas consumption of electricity for storage and marketing was major contributor to marine aquatic ecotoxicity, terrestrial ecotoxicity, photochemical oxidation and acidification. The life cycle assessment of various processes and materials on environmental impact indicators in relation to marketing supply chains revealed that abiotic depletion, global warming, ozone layer depletion, human toxicity, fresh water aquatic ecotoxicity, marine aquatic ecotoxicity, terrestrial ecotoxicity, photochemical oxidation, acidification and eutrophication were highest in marketing supply chains involving the maximum number of chain partners/ intermediaries. In order to minimize the impacts of production and distribution of guava on environment, human health, ecosystem and resources, it is necessary to remodel the production process of agricultural inputs, minimize the use of zinc monosulphate, pesticides, polyvinyl chloride crates and electricity and reduce the number of intermediaries in the supply chain.
Biomonitoring of polyaromatic hydrocarbons (PAHs) by Murraya paniculata (L.) Jack in South Kolkata, West Bengal, India: Spatial and temporal variations
Authors: Mukhopadhyay Shritama , Dutta Ratna , Dhara Aparna , Das Papita 
Published: September 2022

Abstract
Attempts have been made in the present study for ascertaining the content of atmospheric polycyclic aromatic hydrocarbons (PAHs) using passive biosamplers in preference to conventional air sampling methods. Mechanical stirring, sonication, Soxhlet technique and microwave-assisted Soxhlet extraction (MASE) were employed to extract PAHs from an evergreen plant (Murraya paniculata) leaves (having long life-span) sampled from polluted places of South Kolkata, India, with dense population. Effects of extraction methods and operational parameters on the recovery levels of PAHs were also investigated. Purified extracts, acquired through adsorption chromatography, were subjected to GC-MS and HPLC-UV analyses for qualitative and quantitative assessment of PAHs. Spatio-temporal distribution of accumulated PAHs across the sampling sites was monitored over premonsoon, postmonsoon and winter supported by pollutant source characterization. The results displayed that the extraction yields of Soxhlet and MASE were highest among the four techniques. Conditions of extraction with toluene for 6 h were found to be most favourable for PAHs. Total concentrations of PAHs in the foliar samples varied from 200.98 ± 2.72−550.79 ± 10.11 µg g− 1 dry weight, highest values being recorded in the samples of Exide More (EXM) because of daylong inexorable traffic flow/crowding increasing the burden of ambient PAHs. Widespread changes in meteorology exerted influence on seasonal concentrations of PAHs in plant leaves. Foliar accretion of PAHs differed in the study sites with diverse sources of emission from motor vehicles, fossil fuel and biomass burning along with other human interferences.
Somatic embryogenesis in Abies nebrodensis, an endangered Sicilian fir
Authors: Jouini Nourhene , Yahyaoui Emna , Tarraf Waed , Izgu Tolga , Benelli Carla , Lambardi Maurizio , Germanà Maria Antonietta 
Published: September 2022

Abstract
The critically endangered Sicilian fir ( Abies nebrodensis ) (Lojac) Mattei remains alarmingly close to extinction, even after the initially encouraging results previously obtained by the EU LIFE (European LIFE program) funded project in over ten years. Thus, an efficient protocol for in vitro propagation, seed and cryo-banks establishment would be needed to protect and preserve such species. Therefore, s eeds from Abies adult trees were collected and callus was initiated from both zygotic immature and mature embryos, testing different culture media. Embryogenic (EC) and non-embryogenic (NEC) callus were successfully induced from mature embryos with variable frequencies (0% - 40%) in SH (Schenk and Hilderbrandt) medium, supplemented with 1 mg L −1 6benzylaminopurine, 500 mg L −1 glutamine, 1 g L −1 casein, and 1 g L −1 myo-inositol. The effect of donor tree was observed, and callus initiation rate of up to 40% was observed in the mature zygotic embryos issued from donor tree with identification number (IN) 8. The immature embryos remained unresponsive. Maturation of cell lines from trees with IN7, IN8, IN10 and IN 21 was successfully achieved with 10 mg L -1 abscisic acid and polyethylene glycol (PEG-4000, 8%).
Intraarticular steroids as DMARD-sparing agents for juvenile idiopathic arthritis flares: Analysis of the Childhood Arthritis and Rheumatology Research Alliance Registry
Authors: Hahn Timothy , Daymont Carrie , Beukelman Tim , Groh Brandt , Hays Kimberly , Bingham Catherine April , Scalzi Lisabeth 
Published: September 2022

Abstract
Background Children with juvenile idiopathic arthritis (JIA) who achieve a drug free remission often experience a flare of their disease requiring either intraarticular steroids (IAS) or systemic treatment with disease modifying anti-rheumatic drugs (DMARDs). IAS offer an opportunity to recapture disease control and avoid exposure to side effects from systemic immunosuppression. We examined a cohort of patients treated with IAS after drug free remission and report the probability of restarting systemic treatment within 12 months. Methods We analyzed a cohort of patients from the Childhood Arthritis and Rheumatology Research Alliance (CARRA) Registry who received IAS for a flare after a period of drug free remission. Historical factors and clinical characteristics and of the patients including data obtained at the time of treatment were analyzed. Results We identified 46 patients who met the inclusion criteria. Of those 49% had restarted systemic treatment 6 months after IAS injection and 70% had restarted systemic treatment at 12 months. The proportion of patients with prior use of a biologic DMARD was the only factor that differed between patients who restarted systemic treatment those who did not, both at 6 months (79% vs 35%, p < 0.01) and 12 months (81% vs 33%, p < 0.05). Conclusion While IAS are an option for all patients who flare after drug free remission, it may not prevent the need to restart systemic treatment. Prior use of a biologic DMARD may predict lack of success for IAS. Those who previously received methotrexate only, on the other hand, are excellent candidates for IAS.
Belief that addiction is a discrete category is a stronger correlate with stigma than the belief that addiction is biologically based
Authors: Siddiqui Hasan , Rutherford M. D. 
Published: September 2022

AbstractBackground Drug addiction is stigmatized, and this stigma contributes to poor outcomes for individuals with addiction. Researchers have argued that providing genetic explanations of addiction will reduce stigma (Richter et al., 2019), but there has been limited research testing this prediction. Methods We presented participants with news articles that either provided genetic or anti-genetic explanations of addiction. Results Participants’ biological essentialism correlated with stigma in the context of both opioid and methamphetamine addiction. However, participants’ non-biological essentialism was a significantly stronger correlate with stigma. Conclusions This suggests that other essentialist beliefs, like belief that categories are discrete, may be more useful than biological essentialism for understanding addiction stigma.