bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of rearing mode on gastro-intestinal microbiota and development, immunocompetence, sanitary status and growth performance of lambs from birth to two months of age
Authors: Duniere Lysiane , Ruiz Philippe , Lebbaoui Yacine , Guillot Laurie , Bernard Mickael , Forano Evelyne , Chaucheyras-Durand Frédérique 
Published: November 2022

Abstract
Background: Artificial rearing system, commonly used in prolific sheep breeds, is associated to increased mortality and morbidity rates before weaning, which might be linked to perturbations in digestive tract maturation, including microbiota colonization. This study evaluated the effect of rearing mode (mothered or artificially reared) on the establishment of the rumen and intestinal microbiome of lambs from birth to weaning. We also measured immunological and zootechnical parameters to assess lambs’ growth and health. GIT anatomy as well as rumen and intestinal epithelium gene expression were also analysed on weaned animals to assess possible long-term effects of the rearing practice.
Results: Total VFA concentrations were higher in mothered lambs at 2 months of age, while artificially-reared lambs had lower average daily gain, a more degraded sanitary status and lower serum IgG concentration in the early growth phase. Metataxonomic analysis revealed higher richness of bacterial and eukaryote populations in mothered vs artificially-reared lambs in both Rumen and Feces. Beta diversity analysis indicated an evolution of rumen and fecal bacterial communities in mothered lambs with age, not observed in artificially-reared lambs. Important functional microorganisms such as the cellulolytic bacterium Fibrobacter succinogenes and rumen protozoa did not establish correctly before weaning in artificially-reared lambs. Enterobacteriaceae and Escherichia coli were dominant in the fecal microbiota of mothered lambs, but main E. coli virulence genes were not found differential between the two groups, suggesting they are commensal bacteria which could exert aprotective effect against pathogens. The fecal microbiota of artificially-reared lambs had a high proportion of lactic acid bacteria taxa. No difference was observed in mucosa gene expression in the two lamb groups after weaning.
Conclusions: The rearing mode influences gastrointestinal microbiota and health-associated parametersin offspring in early life: rumen maturation was impaired in artificially-reared lambs which also presented altered sanitary status and gut dysbiosis. The first month of age is thus a critical period where the gastrointestinal tract environment and microbiota are particularly unstable and special care should be taken in the management of artificially fed newborn ruminants.
Twenty-year longitudinal evaluation of blood-based DNA methylation biomarkers for early prediction of Alzheimer’s disease
Authors: Hackenhaar Fernanda Schäfer , Josefsson Maria , Adolfsson Annelie Nordin , Landfors Mattias , Kauppi Karolina , Porter Tenielle , Milicic Lidija , Laws Simon M. , Hultdin Magnus , Adolfsson Rolf , Degerman Sofie , Pudas Sara 
Published: November 2022

Abstract
Background
DNA methylation (DNAm) is an epigenetic mechanism reflecting both inherited and environmental influences, and is a promising biomarker of multifactorial aging-related disorders like Alzheimer’s disease (AD). Early prediction of AD is critical, but little is known about the time-course of DNAm biomarkers long before symptom onset.
Methods
The long-term predictive ability of four existing DNAm-based epigenetic age acceleration clocks was tested in a longitudinal case-control sample (50 late-onset AD cases; 51 age- and sex-matched controls) with prospective data up to 16 years prior to clinical onset (mean: 8 years), and a post-onset follow-up. In addition, novel blood-based DNAm biomarkers for AD prediction were generated with epigenome-wide longitudinal linear mixed effects models, as well as sparse partial least squares discriminant analysis applied at time-points 10–16 years pre-onset and 0–7 years post-onset.
Results
Epigenetic age acceleration clocks did not differentiate cases from controls at any point during the 20-year follow up time (ps > 0.05). Our new DNA biomarkers, comprising 73, 7, and 27 CpG sites respectively, had excellent in-sample discriminatory and predictive accuracy on average 8 years prior to clinical onset (AUCs = 71.1–98.2% including age, sex, and white blood cell proportions). The longitudinal panel of CpGs replicated nominally (p = 0.012) in an external cohort (n = 146 cases, 324 controls). However, compared with the established genetic marker APOE ε4 our panel had a limited effect size (OR = 1.38 per 1 SD panel score increase vs. OR = 13.58 for ε4-allele carriage) and discriminatory accuracy in the external cohort (AUC = 77.2% vs. 87.0% for models with age, sex, and white blood cell proportions). A literature review showed low overlap (n = 4) across 3275 CpGs previously reported to be AD-associated in 8 published studies, and no overlap with our currently identified CpGs.
Conclusions
The results extend prior studies showing a limited predictive and prognostic value of epigenetic age acceleration in AD by considering a longer pre-onset follow-up time, and with appropriate control for age, sex, APOE, and white blood cell proportions. The findings further highlight challenges with replicating discriminatory or predictive CpGs across studies.
Targeted chitosan nanobubbles as a strategy to down-regulate microRNA-17 into B-cell lymphoma models
Authors: Capolla Sara , Argenziano Monica , Bozzer Sara , D’Agaro Tiziana , Bittolo Tamara , Leo Luigina , Not Tarcisio , Busato Davide , Bo Michele , Toffoli Giuseppe , Cavalli Roberta , Gattei Valter , Bomben Riccardo , Macor Paolo 
Published: November 2022

Abstract
Background: MicroRNAs represent interesting targets for new therapies because their altered expression influences tumor development and progression. miR-17 is a prototype of onco-miRNA, known to be overexpressed in B-cell non-Hodgkin lymphoma (B-NHL) with peculiar clinic-biological features. AntagomiR molecules have been largely studied to repress the regulatory functions of up-regulated onco-miRNAs, but their clinical use is mainly limited by their rapid degradation, kidney elimination and poor cellular uptake when injected as naked oligonucleotides.
To overcome these problems, we exploited CD20 targeted chitosan nanobubbles (NBs) for a preferential and safe delivery of antagomiR17 to B-NHL cells.
Results: Positively charged 400 nm-sized nanobubbles (NBs) represent a stable and effective nanoplatform for antagomiR encapsulation and specific release into B-NHL cells. NBs rapidly accumulated in tumor microenvironment, but only those conjugated with a targeting system (antiCD20 antibodies) were internalized into B-NHL cells, releasing antagomiR17 in the cytoplasm, both in vitro and in vivo. The result is the down-regulation of miR-17 level and the reduction in tumor burden in a human-mouse B-NHL model, without any documented side effects.
Conclusions: Anti-CD20 targeted NBs investigated in this study showed physico-chemical and stability properties suitable for antagomiR17 delivery in vivo and represent a useful nanoplatform to address B-cell malignancies or other cancers through the modification of their surface with specific targeting antibodies.
New impressive analytical optical soliton solutions to the Schrödinger-Poisson dynamical system against its numerical solutions
Authors: Zahran Emad H.M. , Bekir Ahmet , Ibrahim Reda A. 
Published: November 2022

Abstract
In our current study, we will derive new diverse enormous impressive analytical optical soliton solutions for the Schrödinger-Poisson dynamical system. The proposed model is applied in gravity field with the corresponding quantum state that produces coupling between different gravity states. Moreover, this model has a significant role in the field of many quantum phenomena. Hereby, we will construct diverse forms of the soliton behaviors that arising from this dynamical system via the solitary wave ansatze method. This technique is one of the ansatze methods that doesn’t surrenders to the homogeneous balance and continuously achieves good results. Moreover, we will construct the numerical solutions that are identical for all achieved exact solutions by using two-dimensional differential transform method (TDDTM). The extracted soliton solutions are new compared with that realized before by other authors who used various techniques. The achieved solutions will give new distinct configurations to soliton behaviors arising from this model and show the fact of charges regular distributions on conductors’ materials surface.
Analysis of Surgical Strategies and Efficacy in the Treatment of Os Odontoideum with Atlantoaxial Dislocation
Authors: Yang Baohui , Lu Teng , He xijing , Li Haopeng 
Published: November 2022

Abstract
Background:
There are many classification systems for atlantoaxial dislocation (AAD). Among these systems, the definitions of irreducible AAD remain vague, and its treatments are not unified.
Objective: To explore the surgical strategies and their efficacy for the treatment of os odontoideum (OO) with AAD.
Methods: The clinical data of 56 OO patients with AAD who underwent surgery from January 2017 to June 2021 were retrospectively analyzed. AAD was classified into four types, Type I and type II were treated with posterior fixation and fusion. Type III received posterior fixation and fusion after irreducible dislocations were converted to reducible dislocations by trans lateral mass release or transoral release. Type IV required transoral release for conversion into reducible dislocations before posterior fixation and fusion. The operation time, intraoperative blood loss, and perioperative complications were recorded. The preoperative and postoperative neurological function changes were assessed using the Japanese Orthopedic Association (JOA) score. Postoperative fusion status was assessed by X-ray or CT scan.
Results: There were 40 cases (71%) of type I-II, 14 cases (25%) of type III, and two cases (4%) of type IV AAD. The operation time was 137.5±42.04 min, and the blood loss was 202.85±171.21 mL. One patient with type III died, one with type III underwent revision surgery due to infection, and three patients with type I had further neurological deterioration after operation. Except for the patients who died, all patients were followed up for 12-24 months. The follow-up results showed that sufficient decompression was achieved and that the fixation and fusion were effective. The JOA score increased from 9.58±1.84 points before operation to 13.09±2.68 points at 3 months after operation,14.07±2.83 points at 6 months and 14.25±2.34 at 12 months after operation, all significant differences comparison with preoperative results (P<0.05).
Conclusion: OO patients with irreducible AAD can be treated by trans lateral mass release or transoral release combined with posterior fixation and fusion, while some of those with bony fusion can be treated by transoral release combined with posterior fixation and fusion.
GCPAN: A Group Convolution Network with Pixel Attention Mechanism for Remote Sensing Image Super-resolution
Authors: liu cong , Wang Yaxin 
Published: November 2022

Abstract
Image super-resolution reconstruction techniques, particularly those based on convolutional neural networks (CNN), have recently been successful in remote sensing image reconstruction. However, as we all known that, there are still two limitations, which limit their performance on several remote sensing tasks. Firstly, most of existing methods usually need a large number of parameters to construct their networks, which make the network difficult to train. Moreover, due to the gradient problem and the data degradation problem caused by large parameters, the model will become inefficient and have heavy computational burden. Secondly, the remote sensing image often contains many high frequency and low frequency information and most existing methods are treat them equally. Aiming at these problems, in this paper, we design a novel group convolution network with a pixel attention mechanism named GCPAN to better recover the details of the remote sensing image. First, we use the group convolution to reduce the parameters and computations by diving the feature maps into some groups, however, that may lost the correlation of different features, which is solved by using the channel shuffle after the group convolution. Second, we design a new attention mechanism (pixel attention mechanism) to capture more important information by assigning different weights to each pixel. All of these operations can improve the discriminative learning ability of the network. Our experiments show that our proposed model outperforms the existing state-of-the-art methods both quantitatively and perceptually.
Impact of Nutrition Education on Knowledge and Beliefs about Dietary supplements/Herbal foods: A pre - post intervention study
Authors: Kalkuz Seyma , Goktas Zeynep 
Published: November 2022

Abstract

Background:Dietary supplements/herbal foods are widely recommended in the prevention and treatment of COVID-19 through social media and conventional media platforms, although their effects are unproven. However, studies showed that both general population and health professionals, who recommend these products to the general population, may not be adequately informed about dietary supplements/herbal foods use. The unnecessary use of dietary supplements/herbal foods may cause harmful effects including food-drug interactions and side effects. Hence, this study aims to determine the knowledge and beliefs of health professionals and general population towards the use of dietary supplements/herbal foods in the prevention and treatment of COVID-19 and to evaluate the effectiveness of a nutrition education intervention.Methods:Overall,106 health professionals and 110 general population completed the study.The intervention was administered in 2 sessions for each group through an online video communication application. The main outcomes were tested with self-administered questionnaires. Comparison of the groups before and after the intervention was implemented with McNemar's test for categorical variables and Paired Sample T-test for numerical variables.Results:Total knowledge scores of health professionals (from 3.85 ± 1.60 to 5.51 ± 1.45) and general population (from 8.34 ± 1.45 to 9.39 ± 0.87)improved significantly after the intervention (p=0.000). While most health professionals and general population believed that dietary supplements/herbal foods were effective in the prevention and treatment of COVID-19, this rate decreased significantly after the intervention (p<0.05).Conclusions:Education intervention is effective to inform both health professionals and general population about the effectiveness of dietary supplements/herbal foods in COVID-19. It is important to raise awareness on this topic to prevent the unnecessary use of dietary supplements/herbal foods. Future studies may focus on the development of other educational materials.
Demystification of the true cost of water within industrial facilities
Authors: da Rosa Santos Ana Clara , McCormack David 
Published: November 2022

Abstract
The aim of this work is to provide the necessary steps to acquire the total cost of water at industrial facilities. Therefore, the research was developed in three parts: literature review to identify the available tools related to water management; a case study in the context of the dairy industry; and an analysis and discussion of results achieved. Water applications for process and utility end-users in the manufacturing context require pre- and post-treatment – these are interconnected assets that increase the cost of water, and introduce system inefficiencies, thus, they represent a challenge faced by companies and energy/utility managers. A meta-analysis approach was used to examine data from several independent studies of water management, in order to determine overall trends. The sources utilized during the study were water-related scientific publications, Master theses, books, companies, and standards’ websites. The results of the study highlight that auditing can be used to investigate water flows within productive processes entirely, as well as within individual process units and operations. The present study incorporates water-related thinking about supply and demand costs, opportunities, and strategies, promoting energy systems thinking and providing an internationally replicable solution to calculate the true cost of water.
Prediction and parameter quantitative analysis of side orifice discharge coefficient based on machine learning
Authors: Shen Guiying , Li Shanshan , Parsaie Abbas , Li Guodong , Cao Dingye , Pandey Prashant 
Published: November 2022

AbstractIn this paper, the sparrow search algorithm is used to predict the discharge coefficient (Cd) of the triangular side orifice for the first time. Dimensionless parameters influencing the size of Cd of side orifices are obtained as input values and discharge coefficient as output values of the model. The results show that the determination coefficient R2 is 0.973, the root means square error RMSE is 0.0122, and the average absolute percentage error is 0.010% in the testing phase. The model has high forecast accuracy, strong generalization ability and higher accuracy than other models and traditional empirical formulas. Quantitative analysis by Sobol's method shows that the ratio W/H of top orifice height to side orifice height, Fr of upstream Froude number, and ratio B/L of channel width to a bottom edge length of side orifice are the main factors influencing the discharge capacity of triangular side orifice. The first-order sensitivity coefficient and global sensitivity coefficient are 0.23, 0.11, 0.17 and 0.41, 0.39, 0.35 respectively.
Unusual Combination Of The Distorted Structure And Frozen Internal Mobility In Inactivated Actin Molecule
Authors: Uverskyb Vladimir N. , Turoverov Konstantin K. , Kuznetsova Irina M. , Khaitlina Sofia Yu. 
Published: November 2022

Abstract:
The structural properties of inactivated actin were studied by means of circular dichroism and fluorescence techniques. We have shown that inactivated actin, being denatured, is characterized by a relatively rigid microenvironment of aromatic amino acid residues and exhibits a considerable limitation in the internal mobility oftryptophans. This can be due to the specific self-association of partially folded protein molecules.
Synthesis and Evaluation of Antimicrobial Activity of N-Substituted Indole Derivatives and Molecular Docking Studies
Authors: Dwivedi Ashish Ranjan , Kumar Vijay , Neha  , Jangid Kailash , Devi Bharti , Kulharia Mahesh , Kumar Rakesh , Kumar Vinod 
Published: November 2022

Abstracts:The increasing burden of microbial infection and emerging resistance against the available antimicrobial drugs drives the development of new agents. Two different series of indole-based compounds (VN-1 to VN-18) were synthesized and analyzed for antimicrobial activity by calculating the diameter of the inhibition zone using the broth dilution method and well diffusion method against Escherichia coli (E. coli) and environmental microbes. Most of the compounds displayed good to moderate activity against E. coli, and VN-4 and VN-9 displayed good inhibitory activity against the tested microbes. Molecular docking and binding energy calculation studies of all the synthesized compounds have been performed for targeting FabI, where most of the compounds showed significant interactions with the aromatic nicotinamide moiety of NAD+. In molecular dynamics studies, VN-9 stays inside the binding cavity for sufficient time to induce antimicrobial activity. Thus, these indole-based derivatives may lead to the development of new antimicrobial agents that may act as FabI inhibitors.
Neuroprotective Potency of Safranal Against Neurological
Disorders
Authors: Fazeli Elham , Ghalibaf Mohammad Hossein Eshaghi , Forouzanfar Fatemeh 
Published: November 2022

Abstracts:
A great number of research has been focused on plants as a source of medicine
against many diseases to overcome the many side effects of chemical drugs.
Safranal, one of the main constituents of saffron [Crocus sativus], has a broad spectrum
of pharmacological effects, including anti-inflammatory, antioxidant, and antiapoptotic
effects. The present review elaborates on the current understanding of the
neuroprotective effects of safranal. According to data published so far, safranal has
the potential to exert neuroprotective effects in neurological disorders such as epilepsy,
stroke, multiple sclerosis, Parkinson, and Alzheimer’s disease. Safranal could be
considered a promising therapeutic agent in the future, although there is a great need
for clinical trial studies.
Diindolylmethane Ameliorates Ischemic Stroke-Induced Brain Injury by
Peripheral and Central Mechanisms
Authors: Krishnamurthy Sairam , Ramakrishna Kakarla , Singh Sushil Kumar 
Published: November 2022

Introduction:
Diindolylmethane (DIM), a major acid condensation product of Indole-3-carbinol, is known to inhibit platelet aggregation and thrombosis. The drugs with antiplatelet and antithrombotic activities are used to treat ischemic stroke.

Objective:
The present study investigated the role of DIM on platelet aggregation inhibitory properties in middle cerebral artery occluded (MCAO) rats.

Methods:
DIM (12.5, 25, and 50 mg/kg) was orally administered to MCAO rats for 3 days. Platelet aggregation, platelet cyclic adenosine monophosphate (cAMP), reactive oxygen species (ROS), hydrogen peroxide (H2O2), and serum cyclooxygenase (COX-1), thromboxane B2 (TXB2), and prostaglandin E2 (PGE2), and inflammatory markers were estimated. Further brain structural and functional recovery was evaluated by measuring cerebral blood flow, neurological deficits, brain infarction, blood-brain barrier (BBB) leakage, brain water content, and histological abnormalities.

Results:
DIM significantly ameliorated adenosine diphosphate (ADP), collagen, thrombin, and arachidonic acid-induced platelet aggregation by inhibiting COX-1, TXB2, and PGE2 and elevating cAMP. Further, DIM also alleviated platelet-mediated oxidative stress (ROS and H2O2) and reduced the serum inflammatory markers, tumor necrosis factor-α (TNF-α) and interleukin -6 (IL-6), and increased anti-inflammatory cytokine, IL-10, in MCAO rats

Conclusion:
DIM treatment confers neuroprotection in MCAO rats by inhibition of platelet aggregation, platelet-mediated oxidative stress, and inflammation. Correspondingly, DIM improved cerebral blood flow and reduced neurological deficits, brain infarction, BBB leakage, brain water content, and histopathological abnormalities indicating the preservation of brain structural integrity. Thus, the present study provided preclinical evidence of DIM neuroprotection against ischemic stroke.
The Diverse Network of Brain Histamine in Feeding: Dissect its Functions
in a Circuit-Specific Way
Authors: Xu Lingyu , Lin Wenkai , Zheng Yanrong , Wang Yi , Chen Zhong 
Published: November 2022

Abstracts:
Feeding is an intrinsic and important behavior regulated by complex molecular, cellular and
circuit-level mechanisms, one of which is the brain histaminergic network. In the past decades, many
studies have provided a foundation of knowledge about the relationship between feeding and histamine
receptors, which are deemed to have therapeutic potential but are not successful in treating feeding-
related diseases. Indeed, the histaminergic circuits underlying feeding are poorly understood and
characterized. This review describes current knowledge of histamine in feeding at the receptor level.
Further, we provide insight into putative histamine-involved feeding circuits based on the classic feeding
circuits. Understanding the histaminergic network in a circuit-specific way may be therapeutically
relevant for increasing the drug specificity and precise treatment in feeding-related diseases.