bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Relationship between NMLR and all-cause mortality in patients with chronic obstructive pulmonary disease: National Health and Nutrition Examination Survey, 1999–2018
Authors: Wei Lu , Zhu Chuanqi , Xiao Jianhong , Song Bin , Zhan Xianghui , Guo Guohua 
Published: November 2022

Abstract
Background
Chronic obstructive pulmonary disease (COPD) has one of the highest morbidity and mortality rates worldwide. COPD-related deaths are associated with inflammation and immunity. As one of the emerging indicators of inflammation and immune status, the (neutrophil + monocyte) / lymphocyte ratio (NMLR) may comprehensively reflect the inflammatory and immune status of the body. However, few studies have examined the association between the NMLR and all-cause mortality in patients with COPD.
Objective
The purpose of this study is to explore the relationship between NMLR and all-cause death in patients with COPD.
Method
Data from the National Health and Nutrition Examination Survey (NHANES) 1999–2018 with mortality follow-up through to December 31, 2019 were analyzed. A total of 2045 participants were evaluated. Patients were divided into two groups based on their median NMLR: patients with an NMLR ≤ 2.57 were assigned to the T1 group (n = 1,026), while patients with an NMLR > 2.57 were assigned to the T2 group (n = 1,019). The relationship between the NMLR and all-cause mortality in patients with COPD was assessed using survival curves based on the NHANES recommended weights.
Conclusions
The NMLR is a comprehensive assessment index of airway inflammation and patient immune status. A higher NMLR in patients with COPD is associated with a higher risk of all-cause mortality.
Evaluation of the Effect of High-intensity Light Curing Device on Micro-leakage of Pits and Fissures Sealants
Authors: Alzoubi Hasan , Rajab Malek , Bshara Nada 
Published: November 2022

Abstract
INTRODUCTION: Reducing treatment time is one of the most important trends in modern dentistry This study provides evidence to help clinicians make choices about the appropriate light-curing system for resin sealants.
AIM: This study aimed to Compare the micro-leakage around the resin sealants when using both high and conventional intensity light-curing systems.
MATERIALS AND METHODS: The study sample consisted of 30 upper premolars that were divided into two equal groups according to the light-curing system used: Group 1: High-Intensity light-curing System and Group 2: The conventional light-curing System. The teeth were immersed in Methylene Blue and the degree of micro-leakage was determined according to the amount of pigment leakage.
RESULTS: The mean of micro-leakage in the high-intensity group (1.33±1.29) was less than in the conventional intensity group (1.63±1.29) without any statistically significant differences (P-value = 0.320).
CONCLUSION: The high-strength light-curing system can be a good and acceptable alternative to conventional light-curing Systems in the curing of pits and fissures sealants.
A novel sparrow search algorithm with integrates spawning strategy
Authors: Zhang Jiaxiang , Li Linwei , Zhang Huanlong , Wang Fengxian , Tian Yangyang 
Published: November 2022

Abstract
The sparrow search algorithm (SSA), which has recently come under more scrutiny in the intelligent optimization groups. Nevertheless, the high randomicity and local optimum problems restrict the application of the algorithm. We provide a novel mixed sparrow search algorithm (NSSA) in this study that incorporates spawning techniques. Firstly, the good point set theory is suggested as an alternative to the conventional stochastic way to find the initial individual, this method can make the initial population more uniform allocation in the search space. Secondly, the oviposition strategy of cuckoo algorithm is integrated into the discovery phase, in which the global search capability is enhanced. This allows the algorithm to avoid a sudden drop in population diversity and precocious convergence. Then we use levy flight and brownian motion to disturb the individual's position dimension by dimension, and improve the power to escape from local optimal values in the later iterations of the algorithm. Finally, by contrasting the NSSA and SSA, DE, ALO and GOA algorithms with 12 common assessment functions and CEC-2017 test functions, the efficacy superiority and of the proposed scheme are validated.
Bidirectional Dynamic Neural Networks with Physical Analyzability
Authors: Li Changjun , Zhao Fei , Lan Xuguang , Tian Zhiqiang , Tao Tao , Mei Xuesong 
Published: November 2022

Abstract
The rapid growth in research exploiting deep learning to predict mechanical systems has revealed a new route for system identification; however, the analytic model as a white box has not been replaced in applications because of its open physical information. In contrast, the models generated by end-to-end learning usually lack the ability of physical analysis, which makes them inapplicable in many situations. Consequently, high-accuracy modeling with physical analyzability becomes a necessity. In this paper, we introduce bidirectional dynamic neural networks, a deep learning framework that can infer the dynamics of physical systems from control signals and observed state trajectories. Based on forward dynamics, we train the neural ordinary differential equations in a trajectory backtracking algorithm. With the trained model, the inverse dynamics can be calculated and based on Lagrangian Mechanics, the physical parameters of the mechanical system can be estimated, including inertia, Coriolis and centrifugal forces, and gravity. As a result, the model can seamlessly incorporate prior knowledge, learn unknown dynamics without human intervention, and provide information as transparent as analytic models. We demonstrate our method on simulated 2-axis and 6-axis robots to evaluate model accuracy, including physical parameters and verified its applicability on real 7-axis robots. The experimental results show that this method is superior to the existing methods. This framework provides a new idea for system identification by providing interpretable, physically consistent models for physical systems.
Kannada translation and cross-cultural adaptation of the SarQol®: sarcopenia specific quality of life questionnaire
Authors: Kumar Prabal , Umakanth Shashikiran , Girish N 
Published: November 2022

Abstract
Background
Sarcopenia Quality of life questionnaire (SarQol) in Indian vernacular language is limited to Hindi, Marathi, and Bengali.
Objective
To translate and cross-culturally adapt the original Sarcopenia Quality of Life Questionnaire (SarQol) to the Kannada language
Methods
The rights owners of the SarQoL questionnaire granted permission for translation and cross-cultural adaption, and the translation was articulated in five stages: (i) two initial translations from English to Kannada; (ii) synthesis of the two translations; (iii) backward translations; (iv) expert committee to compare the backward translations with the original questionnaire and (v) pre-test.
Result
Six items identified in phase I were rectified in second phase. There found to be thirteen major and nine minor discrepancies between back-translated and original questionnaire. Expert committee meeting addressed the discrepancy using the liberal and literal methods to maintain the content and semantic equivalence. Nineteen participants were recruited for pilot testing. To assess for the presence of sarcopenia, participants (n = 10 sarcopenic, n = 9 non-sarcopenic) responded to the Strength, Ambulation, Rising from a chair, Stair climbing and history of Falling (SARC-F). Participants (n = 19, Males/Females: 11/8; Sarcopenic/Non-sarcopenic, mean (SD) age: 71.8 (8.48) years/68.22 (6.14) years) provided suggestions and feedback for seven items which were discussed with the experts and final SarQol-Kannada version was produced.
Conclusion
The Kannada version of the SarQol questionnaire is comparable with the original and after the validation of psychometric properties, it would be a useful tool to assess the quality of life of Kannada-speaking sarcopenic older adults in clinical and research settings.
α-lipoic acid and Propolis shield against lipopolysaccharide-induced behavioral deficits, depression and neuro-inflammation via regulating GR7M/BDNF/Homer/PI3K signaling pathways in rat model
Authors: Kadry Mai OSMAN , Abdel-Megeed Rehab M. 
Published: November 2022

Abstract

Copious pharmacological agents targeting monoamine neurotransmission merit the management of mental illnesses nevertheless, they possess concomitant side effects. Regulating BDNF/PI3K/GR7M/AKT/Homer pathways may constitute a vital signaling center in the subcellular integration of the synaptic neurotransmission. These pathways similarly modulate neuronal cell proliferation, plasticity and migration. As enzymes involved in cell survival and neuroplasticity are relevant to neurotrophic factor deregulation, the brain-derived neurotrophic (BDNF), Metabotropic glutamate receptor type 7 (GRM7), Phosphoinisitol kinase- 3 (PIK3), Serine/Threonine Kinase 1 (AKT), and Homer1 may afford an important signaling for neuro-protection in depression. This article summarized the involvement of PI3K/AKT/GR7M/BDNF/ Homer pathways in cell signaling of neuronal cells in mental illnesses. Depression has been identified as a ubiquitous and severe psychiatric mood neurobehavioral disorder. Neuro-inflammation and AKT/PI3K activation are known to be involved in the pathology of depression. The present study aimed to investigate the therapeutic index and potential mechanism of α-lipoic acid, Burdock and propolis in the lipopolysaccharide (LPS)-induced depression in rat model. α-lipoic acid, Burdock and propolis were injected orally, for 1 month post LPS injection daily. RNA gene expression of BDNF/PI3K/GR7M/AKT/Homer-1 was investigated. In results, α-lipoic acid, Burdock and propolis treatment exerted preventive effects versus LPS-induced depression. The aforementioned treatments increased the expression of PI3K/AKT/GR7M/Homer1 but decreased the ROS generation levels and reduced the expression of BDNF in LPS-induced depressive behavior in rats. In conclusion: These findings suggested that α-lipoic acid, Burdock and propolis exerted a preventive effect via GRM7/BDNF/AKT/PI3K signaling pathways in LPS-induced depressive behavior in rats.
CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis
Authors: Zhang Guoyi , Wang Junyu , Li Yiwen , Shang Guangdong 
Published: November 2022

Abstract
Site-directed mutagenesis and saturation mutagenesis are essential means for gene and protein studies. For plasmid-based gene mutation, PCR and overlap-extension PCR are still the most often used methods; however, the methods usually involve time-consuming and labor-intensive gene cloning steps. When the plasmid size is larger than 5 kb, PCR may fail or the yield may be too low to meet cloning demand. Still, for PCR-mediated saturation mutagenesis, one experiment may not enough to get all the 20 variants. Recombineering sidesteps the complicated cloning process by electroporating mutagenic DNA into recombinase-proficient cells followed by selection of mutational clones. However, the low recombineering efficiency makes it necessary to screen a large number of clones. Herein, we report CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis (CRM) and saturation mutagenesis (CRSM) strategies. The CRM and CRSM require only the co-electroporation of target plasmid, sgRNA expression plasmid and mutagenic oligonucleotide into Escherichia coli cells with chromosome-based induced expression of recombinase and Cas9, followed by cells culture, plasmid extraction and characterization. We firstly established CRM via ampicillin resistance gene repair, then with N-acetyl‑D‑neuraminic acid aldolase as target, we performed CRM of E192N, CRM of S208G, CRM of both E192 and S208G, CRSM of E192, and CRSM of S208. Both CRM and CRSM efficiencies were as high as 100%, and all twenty saturation mutagenesis variants were obtained via single CRSM experiment. The highly efficient and straightforward CRM and CRSM approaches have the potential to be general plasmid mutagenesis tools.
ALBEDO EFFECTS IN THE ELLIPTIC RESTRICTED THREE-BODY PROBLEM UNDER AN OBLATE PRIMARY, A TRIAXIAL SECONDARY AND A POTENTIAL DUE TO BELT IN THE EARTH-MOON SYSTEM.
Authors: Singh Jagadish , Tyokyaa Richard Kanshio 
Published: November 2022

Abstract
We have examined the effects of Albedo in the elliptic restricted three-body problem under an oblate primary, a triaxial secondary and a potential due to belt for the Earth-Moon system. We have found that as the perturbed parameters increases, the possible boundary regions of the primary come closer to one other, allowing particles to freely travel from one region to the next and possibly merging the permissible regions. Our study has revealed that, the formation of triangular libration points depend on the Albedo effects, semi-major axis, eccentricity of the orbits, triaxiallity and the potential due to belt. As the aforementioned parameters increase, the triangular positions \({L}_{4}\) and \({L}_{5}\) move towards the centre of origin in cases 1, 2, 3, 4 and away from the centre of the origin in cases 5, 6 and 7. Considering the range of a stable and unstable libration point for the problem under study given as \(0<\mu <{\mu }_{c}\) for stable libration points and \({\mu }_{c}\le \mu \le \frac{1}{2}\) for unstable libration points, our study has established that the triangular libration points are respectively stable and unstable for cases 1, 2, 6 and cases 3, 4, 5, 7. Our study has also revealed that each set of values has at least one characteristic complex root with a positive real part. Hence, the triangular libration points for the Earth-Moon system are unstable in the sense of Lyapunov. The Earth-Moon system's Poincare Surface of Section (PSS) has demonstrated that a small change in the initial conditions, the semi-major axis, and the eccentricity of the orbits have affected the system's behavior dramatically. Further, it is seen that a chaotic dynamical behavior of the system results into either regular or irregular orbits.
Pain Trajectory after Short-Stay Anorectal Surgery: A Prospective Observational Study
Authors: Zhang Yujiao , Li Lili , Yong Yue , Zhou Yalan , Yin Zhiyu , Wang Jing , Mei Ling , Song Wei , Wang Jian , Song Jiangang 
Published: November 2022

Abstract
OBJECTIVE: The dense distribution of perianal nerves, having to defecate, and underappreciated analgesia make the postoperative pain of anorectal surgery significantly serious. However, the evolution of pain after anorectal surgery has not been well characterized. The main objective is to evaluate patterns in acute postoperative pain in patients undergoing short-stay anorectal surgery.The main objective is to evaluate patterns in acute postoperative pain in patients undergoing short-stay anorectal surgery.
Methods: The study included 217 patients who underwent anorectal surgery for hemorrhoids and anorectal fistula. The VAS pain scale was used to record the pain across postoperative days 1 through 10. The primary analysis used group-based trajectory modeling to estimate the trajectory of postoperative pain. Secondary analysis examined the relationships between sociodemographic, types of surgery, preoperative anxiety score and pain behavior score, and postoperative analgesics and pain trajectories.
RESULTS: Three distinct postoperative pain trajectories were determined. Many patients (57.0%) belonged to the high-low group, followed by the low group (23.3%) and the low-moderate-low group (19.7%). Moreover, 62.7% of patients reported pain stable and sustained moderate-to-high over the first 7 days after surgery, while 97% had decreased to mild pain by the 10th day. Hemorrhoidectomy (odds ratio, 0.15), higher anxiety (odds ratio, 3.26), and higher preoperative pain behavior score (odds ratio, 3.15), were associated with an increased likelihood of being in the high pain trajectory in multivariate analysis. The pain trajectory group was related to postoperative analgesic use (P<0.001), with the high-low group needing more nonsteroidal analgesics.
CONCLUSIONS: There are three obvious pain trajectories after anorectal surgery for hemorrhoids and anal fistula, including an unreported low-moderate-low type. More than 60% of patients maintained moderate and severe pain within 7 days after the operation. These postoperative pain trajectories were predominantly defined by surgery factors and patient factors.
Lessons Learnt from Emergency Online Face-to-Face when Back on Campus from Transactional Distance Theory Perspective
Authors: Kozlova Inna 
Published: November 2022

Abstract

In language learning, e-learning is often contrasted to on-campus face-to-face (F2F) in what is known as media studies. This approach ignores the fact that most university courses are already blended, with synchronous element accounting for approximately 1/3 of the student’s workload, with the other 2/3 being asynchronous and delivered mostly online. Focusing on the synchronous part only allows us to compare the two distinct modes of its delivery: on campus and by videoconferencing, with its corresponding effects on the rest of the course. This single-case study is conducted with a group of first-year university studying a foreign language for translators and interpreters via videoconferencing in the first semester and on campus in the second semester. The research design is cross-sectional with data proceeding from course statistics and a final questionnaire. The conceptual framework is based on Transactional Distance Theory by Moore and Zone of Proximal Development Theory by Vygotsky. Our students regarded class dialogue in online F2F as being maintained though affected by technology issues additional to those present in on-campus F2F communication. Moreover, students valued positively the fact of having more time available for study, they improved their autonomy and reported feeling more comfortable when had classes online. The traditional universities after the pandemic face a new technological panorama, with students happy to socialize on campus but already conscious of existing alternatives to on-campus teaching. Deeper research into class dialogue, on-campus, online and mixed, is urgently needed for the universities to decide whether they should adopt these alternatives.
Breast cancer spheroids can model phenotypic heterogeneity of cancer cells to evaluate targeted therapies.
Authors: mahmoud loay , cougnoux antony , bekiari christina , teba paloma araceli ruiz de castroviejo , marrahi anissa el , panneau guilhem , gsell louise , hausser jean 
Published: November 2022

Abstract
Breast cancer (BC) is the most commonly diagnosed cancer among women. Prognosis has improved over the years, to a large extent, owing to personalized therapy informed by molecular profiling of hormone receptors. However, there is a need for new therapeutic approaches for a subgroup of BCs lacking molecular markers, the Triple Negative Breast Cancer (TNBC) subgroup. TNBC is the most aggressive type of BC, lacks an effective standard of care, shows high levels of resistance and relapse is often inevitable. High resistance to therapy has been hypothesized to be associated with high intratumoral phenotypic heterogeneity. To characterize and treat this phenotypic heterogeneity, we optimized a whole-mount staining and image analysis protocol for three-dimensions (3D) spheroids. Applying this protocol to TNBC spheroids identifies the phenotypes of single cancer cells within their spatial context. Cells with dividing, migrating, and high mitochondrial mass phenotypes localize in the outer region of the spheroid. These phenotypes are specifically targeted by Paclitaxel, Trametinib, and Everolimus, respectively, in a dose-dependent manner. Single agents cannot target all phenotypes at the same time, or only at high concentrations which can cause adverse effects. To reduce these adverse effects, we find that targeting multiple cell phenotypes — by combining Trametinib and Everolimus for example — achieves higher cytotoxicity at lower doses. These findings suggest a rational approach to design treatments against heterogeneous tumors and potentially reduce adverse effects.
Is early bilateral compression ultrasonography and D-dimer monitoring appropriately for prophylaxis and diagnosis of deep venous thrombosis after Cesarean section women: a single-center observation study of Chinese Han population
Authors: chen xiuying , Sun yun , Jiang Haiyan , Zhou aiping , Du minmin , Zhao Ying , Zhao Baihui 
Published: November 2022

Abstract
Objective
Venous thromboembolism (VTE) is most common in parturient after cesarean section (CS), the purpose of this study was to explore the practical value of bilateral compression ultrasonography of the legs along with D-dimer monitoring in the prevention and early diagnosis of VTE in the Han Chinese population.
Materials and methods
742 women with ultrasound examination and D-dimer testing on the first day after CS were involved in our prospective observation study in southeast China. Except for normal and thrombosis, other ultrasonic findings were defined as abnormal. D-dimer ≥3 mg/l was used as the VTE alarm value in our study, and early ambulation and mechanical prophylaxis were recommended to all women. A sequential diagnostic strategy was based on the 2015 RCOG VTE risk-assessment tool, parturient with abnormal ultrasound and D-dimer ≥ 3 mg/l were given pharmacological VTE prophylaxis with LMWH and all high-risk women also treated with preventive LMWH. The statistical analysis was carried out using R statistical software. Two-sided P value < 0.05 was deemed statistically significant.
Result
There are 15 VTE cases and 727 women without VTE were observed, the patient age and mean D-dimer level during the day after Cesarean section were significant difference between two groups. The overall rate of VTE after CS was 2.02%, and most of them (66.7%) were asymptomatic. 10 patients were diagnosed at the first postpartum day. None maternal deaths occurred in the study cohort. 41 parturient had abnormal ultrasound and D-dimer ≥ 3 mg/l, although they had pharmacological VTE prophylaxis with LMWH, 2 (3.85%) of them in high risk group were finally diagnosed VTE. 229 parturient (30.86%) had normal ultrasound and D-dimer < 3 mg/l on the first day after CS, and no VTE occurred in the postpartum follow-up. Only 20.62% parturient had met our criterion for prophylactic anticoagulation while 78.03% according to RCOG’s recommendation.
Conclusion
The strategy of timely routine bilateral compression ultrasonography (CUS) of lower limbs and D-dimer monitoring is conductive to early diagnosis, early prevention and early treatment of VTE, and greatly reduced the use of LWMH.