bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cyclin-Dependent Kinase Inhibitors Function as Potential Immune Regulators via Inducing Pyroptosis in Triple Negative Breast Cancer
Authors: Xu Tao , Wang Zhen , Liu Jiahao , Wang Ge , Zhou Dongchen , Du Yaying , Li Xingrui , Xia Yu , Gao Qinglei 
Published: June 2022

BackgroundImmunotherapy is the most promising treatment in triple-negative breast cancer (TNBC), and its efficiency is largely dependent on the intra-tumoral immune cells infiltrations. Thus, novel ways to assist immunotherapy by increasing immune cell infiltrations were highly desirable.MethodsTo find key immune-related genes and discover novel immune-evoking molecules, gene expression profiles of TNBC were downloaded from Gene Expression Omnibus (GEO). Single-sample gene set enrichment analysis (ssGSEA) and Weighted Gene Co-expression Network Analysis (WGCNA) were conducted to identified hub genes. The CMap database was used subsequently to predicate potential drugs that can modulate the overall hub gene expression network. In vitro experiments were conducted to assess the anti-tumor activity and the pyroptosis phenotypes induced by GW-8510.ResultsGene expression profiles of 198 TNBC patients were downloaded from GEO dataset GSE76124, and ssGSEA was used to divide them into Immune Cell Proficiency (ICP) group and Immune Cell Deficiency (ICD) group. Hub differential expressed gene modules between two groups were identified by WGCNA and then annotated by Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. A cyclin-dependent kinase (CDK) 2 inhibitor, GW-8510 was then identified by the CMap database and further investigated. Treatment with GW-8510 resulted in potent inhibition of TNBC cell lines. More importantly, in vitro and in vivo studies confirmed that GW-8510 and other CDK inhibitors (Dinaciclib, and Palbociclib) can induce pyroptosis by activating caspase-3 and GSDME, which might be the mechanism for their immune regulation potentials.ConclusionGW-8510, as well as other CDK inhibitors, might serve as potential immune regulators and pyroptosis promotors in TNBC.
Systematic Bibliometric and Visualized Analysis of Research Hotspots and Trends on the Application of Artificial Intelligence in Ophthalmic Disease Diagnosis
Authors: Zhao Junqiang , Lu Yi , Zhu Shaojun , Li Keran , Jiang Qin , Yang Weihua 
Published: June 2022

Background: Artificial intelligence (AI) has been used in the research of ophthalmic disease diagnosis, and it may have an impact on medical and ophthalmic practice in the future. This study explores the general application and research frontier of artificial intelligence in ophthalmic disease detection.Methods: Citation data were downloaded from the Web of Science Core Collection database to evaluate the extent of the application of Artificial intelligence in ophthalmic disease diagnosis in publications from 1 January 2012, to 31 December 2021. This information was analyzed using CiteSpace.5.8. R3 and Vosviewer.Results: A total of 1,498 publications from 95 areas were examined, of which the United States was determined to be the most influential country in this research field. The largest cluster labeled “Brownian motion” was used prior to the application of AI for ophthalmic diagnosis from 2007 to 2017, and was an active topic during this period. The burst keywords in the period from 2020 to 2021 were system, disease, and model.Conclusion: The focus of artificial intelligence research in ophthalmic disease diagnosis has transitioned from the development of AI algorithms and the analysis of abnormal eye physiological structure to the investigation of more mature ophthalmic disease diagnosis systems. However, there is a need for further studies in ophthalmology and computer engineering.
A Poultry Subclinical Necrotic Enteritis Disease Model Based on Natural Clostridium perfringens Uptake
Authors: He Wanwei , Goes Emanuele C. , Wakaruk Jeremy , Barreda Daniel R. , Korver Douglas R. 
Published: June 2022

Necrotic enteritis (NE) in poultry is an opportunistic infection caused by Clostridium perfringens. Well-known as a multifactorial disease, NE development is under the influence of a wide range of environmental risk factors that promote the proliferation of pathogenic C. perfringens at the expense of nonpathogenic strains. Current in vivo NE challenge models typically incorporate pre-exposure to disease risk factors, in combination with exogenous C. perfringens inoculation. Our goal was to enhance current models using a natural uptake of C. perfringens from the barn environment to produce a subclinical infection. We incorporated access to litter, coccidial exposure (either 10× or 15× of the manufacturer-recommended Coccivac B52 Eimeria vaccine challenge; provided unspecified doses of E. acervulina, E. mivati, E. tenella, and two strains of E. maxima), feed composition, and feed withdrawal stress, and achieved the commonly observed NE infection peak at 3 weeks post-hatch. NE severity was evaluated based on gut lesion pathology, clinical signs, and mortality rate. Under cage-reared conditions, 15× coccidial vaccine-challenged birds showed overall NE lesion prevalence that was 8-fold higher than 10× coccidial vaccine-challenged birds. NE-associated mortality was observed only in a floor-reared flock after a 15× coccidial vaccine challenge.
Adolescent and Young Adult Initiated Discussions of Advance Care Planning: Family Member, Friend and Health Care Provider Perspectives
Authors: Bedoya Sima Z. , Fry Abigail , Gordon Mallorie L. , Lyon Maureen E. , Thompkins Jessica , Fasciano Karen , Malinowski Paige , Heath Corey , Sender Leonard , Zabokrtsky Keri , Pao Maryland , Wiener Lori 
Published: June 2022

Background and AimsEnd-of-life (EoL) discussions can be difficult for seriously ill adolescents and young adults (AYAs). Researchers aimed to determine whether completing Voicing My CHOiCES (VMC)—a research-informed advance care planning (ACP) guide—increased communication with family, friends, or health care providers (HCPs), and to evaluate the experience of those with whom VMC was shared.MethodsFamily, friends, or HCPs who the AYAs had shared their completed VMC with were administered structured interviews to assess their perception of the ACP discussion, changes in their relationship, conversation quality, and whether the discussion prompted changes in care. Open-ended responses underwent thematic analysis.ResultsOne-month post-completion, 65.1% of AYA had shared VMC completion with a family member, 22.6% with a friend, and 8.9% with an HCP. Among a sample of respondents, family (47%) and friends (33%) reported a positive change in their relationship with the AYA. Participant descriptions of the experience fell into five themes: positive experience (47%), difficult experience (44%), appreciated a guide to facilitate discussion (35%), provided relief (21%), and created worry/anxiety (9%). Only 1 HCP noted a treatment change. Family (76%), friends (67%), and HCP (50%) did not think the AYA would have discussed EoL preferences without completing VMC.ConclusionsVMC has potential to enhance communication about ACP between AYA and their family and friends, though less frequently with HCPs. Participants reported a positive change in their relationship with the AYA after discussing VMC, and described experiencing the conversation as favorable, even when also emotionally difficult.
Surgical Site Infection Following Single-Port Appendectomy: A Systematic Review of the Literature and Meta-Analysis
Authors: Köhler Franziska , Reese Lena , Kastner Carolin , Hendricks Anne , Müller Sophie , Lock Johan F. , Germer Christoph-Thomas , Wiegering Armin 
Published: June 2022

IntroductionSurgical site infections (SSIs) are one of the most common postoperative complications after appendectomy leading to recurrent surgery, prolonged hospital stay, and the use of antibiotics. Numerous studies and meta-analyses have been published on the effect of open versus conventional laparoscopic appendectomy (CLA) reporting faster postoperative recovery and less postoperative pain for CLA. A development from CLA has been the single-port appendectomy (SPA), associated with a better cosmesis but seemingly having a higher risk of wound infections. The aim of this systematic literature review and meta-analysis is to investigate whether reduced port or SPA alters the ratio of SSIs.MethodsPubmed, Embase, and Cochrane databases were screened for suitable articles. All articles published between January 1, 2002, and March 23, 2022, were included. Articles regarding children below the age of 18 were excluded as well as manuscripts that investigated solemnly open appendectomies. Articles were screened for inclusion criteria by two independent authors. Incidence of SSI was the primary outcome. Duration of operation and length of hospital stay were defined as secondary outcomes.ResultsA total of 25 studies were found through a database search describing 5484 patients. A total of 2749 patients received SPA and 2735 received CLA. There was no statistical difference in the rate of SSI (P = 0.98). A total of 22 studies including 4699 patients reported the duration of operation (2223 SPA and 2476 CLA). There was a significantly shorter operation time seen in CLA. The length of hospital stay was reported in 23 studies (4735 patients: 2235 SPA and 2500 CLA). A shorter hospital stay was seen in the SPA group (P &lt; 0.00001). Separately performed analysis of randomized controlled trials could not confirm this effect (P = 0.29).DiscussionSPA is an equally safe procedure considering SSI compared to CLA and does not lead to an increased risk of SSI. A longer operation time for SPA and a minor difference in the length of stay does lead to the use of SPA in selected patients only.
A Combination of Lactic Acid Bacteria and Molasses Improves Fermentation Quality, Chemical Composition, Physicochemical Structure, in vitro Degradability and Rumen Microbiota Colonization of Rice Straw
Authors: Chen Xu , Ma Yulin , Khan Muhammad Zahoor , Xiao Jianxin , Alugongo Gibson Maswayi , Li Shengli , Wang Yajing , Cao Zhijun 
Published: June 2022

AimsThis study aims to evaluate the effect of lactic acid bacteria (LAB) and LAB-molasses (LAB + M) combination on the fermentation quality, chemical composition, physicochemical properties, in vitro degradability of rice straw and the characteristics of rumen microbial colonization on rice straw surface.Methods and ResultsThere were three pretreatments, including control (not treated, Con), treated with LAB, or LAB + M. The results showed that both LAB and LAB + M treatments altered the physical and chemical structures of rice straw and were revealed by scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD) spectroscopy, respectively. Moreover, both LAB and LAB + M pretreated rice straw increased the crude protein (CP) content, dry matter (DM) recovery, and in vitro digestibility and decreased the pH value, neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents. The LAB + M pretreated rice straw increased the gas production (GP72) and rumen microbial colonization on the rice straw surface.ConclusionsIt is observed that LAB + M treatment could increase digestibility and the rumen microbial colonization on the rice straw surface. Therefore, LAB + M treatment can provide an alternative strategy to improve the quality of rice straw. Significance and impact of the study: This study provides an optimal pretreatment to improve the rice straw digestibility and rumen microbial colonization.
Resposta cardiorrespiratória de paraplégicos pelo teste de membro superior: série de casos
Authors: Floripes de Souza Meretica Larissa , Souza Danielly Fernanda de , Silva Juliana Fernandes da , Nascimento Luisa Kulkamp do , Fernandes Maria Vitória Fabrini , Silva Beatriz Almeida , Rodrigues Dionatan Costa , Méndes Maria Verónica Gonzáles , Moraes Gabriela Fóz , Souza Roger Burgo de 
Published: June 2022

Introdução. Indivíduos com lesão da medula espinal (LME) apresentam alterações da capacidade cardiorrespiratória e importante estresse dos membros superiores durante as atividades. Objetivo. Analisar as respostas cardiorrespiratórias de indivíduos paraplégicos submetidos ao teste de exercício de membros superiores sem apoio (UULEX). Método. Séire de casos com quatro indivíduos paraplégicos, nível neurológico T4 e T10. Todos os participantes realizaram o teste UULEX. As variáveis frequência cardíaca (FC), frequência respiratória (FR), pressão arterial sistólica (PAS), pressão arterial diastólica (PAD), Escala de Borg fadiga (BF) e Borg dispneia (BD) foram coletadas antes e após teste. Resultados. A maioria das variáveis alteraram seus valores após o UULEX. Conclusão. As respostas cardiorrespiratórias encontradas foram compatíveis com respostas fisiológicas normais durante uma atividade física.
Patologias no concreto armado em elementos de projetos estruturais: uma revisão / Pathologies in reinforced concrete in elements of structural projects: a review
Authors: Silva Maria Eduarda de Almeida , Da Silva Elzelis Muller , Quaresma Danielly da Silva , Nunes Lucas Mateus Coelho , Passos Inaray de Sousa , Cordeiro Débora Reis , Caldas Ágata Maise de Jesus , Ogorodnik Maria Eduarda Assunção 
Published: June 2022

Embora os avanços tecnológicos na área da engenharia civil tenham avançado , muitas estruturas apresentam um desempenho não satisfatório, devido a erros de projeto, má qualidade dos materiais, falta de manutenção e dentre muitos outros fatores que contribuem para a degradação da estrutura. Portanto, o presente estudo apresenta as patologias no concreto armado que ocorrem nos principais grandes elementos de projeto estrutural como as fundações, as lajes, as vigas e os pilares. Geralmente as manifestações patológicas apresentam características comuns que permitem aos especialistas determinar a origem que conduziram ao surgimento, a causa e as consequências para a estrutura caso não haja intervenção corretiva. Através de revisão bibliográfica o estudo apresenta as seguintes patologias, a corrosão, as fissuras, o concreto segregado ou mal vibrado e o desgaste superficial do concreto, bem como sua origem, causa, e seus tipos .Além disso, apresenta os tratamentos para reforçar estruturas que apresentam patologias, de modo a trazer segurança e durabilidade à estrutura. Também ressalta-se a importância da prevenção e manutenção das estruturas , sob fiscalização eficiente e do controle de qualidade de todas as áreas envolvidas nos projetos de construção civil, ou seja, todas as áreas do processo de construção pautadas na viabilidade social, econômica e ambiental.
Monogenic Diabetes Associated with the Glucose Gene Mutation: a Clinical Case of Early Diagnosis and a Brief Review of Literature
Authors: Солнцева А.В. , Беляева Е.А. 
Published: June 2022

Сахарный диабет взрослого типа у молодых (MODY) является наиболее распространенной формой моногенного диабета. Заболевание возникает в результате мутации одного из генов, которые участвуют в синтезе и секреции инсулина. На сегодняшний день выделено 14 подтипов MODY, классифицированных по этиологической мутации. Один из часто встречающихся вариантов заболевания вызван инактивирующей мутацией гена фермента глюкокиназы. Для него характерна бессимптомная гипергликемия натощак, которая в большинстве случаев выявляется при проведении профилактических осмотров. Секреция эндогенного инсулина остается сохранной. При лабораторном исследовании уровни антител к различным структурам островковых клеток ниже диагностического значения. Окончательный диагноз верифицируется на основании молекулярно-генетического исследования.Значительная вариабельность клинико-лабораторных показателей пациентов затрудняет своевременную постановку диагноза. Раннее выявление заболевания позволяет выбрать рациональную тактику наблюдения и лечения.
Maturity onset diabetes of the young (MODY) is the most common form of monogenic diabetes. The disease occurs as a result of a mutation in one of the genes involved in the synthesis and secretion of insulin. To date, 14 MODY subtypes have been identified, classified by etiological mutation. One of the most common variants of the disease is caused by an inactivating mutation of the glucokinase gene. It is characterized by asymptomatic fasting hyperglycemia, which in most cases is detected during preventive examinations. The secretion of endogenous insulin remains intact. In a laboratory study, the levels of antibodies to various structures of the islet cells are below the diagnostic value. The verification of final diagnosis based on molecular genetic research.Significant variability of clinical and laboratory parameters of patients makes it difficult to make a timely diagnosis. Early detection of the disease allows to choose a rational tactics for monitoring and treating.
РОЛЬ СУЧАСНОЇ ХУДОЖНЬОЇ УКРАЇНСЬКОЇ ПІДЛІТКОВОЇ ЛІТЕРАТУРИ У ФОРМУВАННІ ЦІННІСНИХ ОРІЄНТАЦІЙ УЧНІВ СЕРЕДНЬОГО ПІДЛІТКОВОГО ВІКУ
Authors: Мельникович Галина 
Published: June 2022

У статті наголошено, що аксіологічна сутність художньої української підліткової літератури презентується природньо не нав’язливим переходом гуманістично-ціннісних орієнтацій у внутрішнє надбання особистості підлітка середнього віку. Адже саме у цьому віковому періоді, йому вкрай необхідні для наслідування, у результаті осмисленого читання, позитивні вчинки героїв художніх творів, сприйняті не лише на когнітивному й емоційному рівнях, а й у проявах власної поведінки.
Особливу увагу акцентовано на тому, що аксіологічний вплив художньої підліткової літератури набуває більшої значимості, зумовленої специфікою сприймання прочитаного підлітками середнього віку. Доведено, що визначальна роль української художньої підліткової літератури для формування ціннісних орієнтацій підлітків середнього віку полягає в тому, що художнє слово призводить до великих змін в емоційній сфері читача, сприяє спрямованості його реакцій на різні життєві події, змінює його ставлення до навколишнього світу й самого себе. Завдяки літературним можливостям у процесі читання підліток відображає у своїй уяві певну картину, конкретну ситуацію, образ, переживає описані події, і чим сильніше його переживання, тим багатше його почуття і уявлення про дійсність. Підліток неначе проникає всередину подій художнього твору, ніби стає їх учасником. Отож, набуваючи живого змісту моральні норми й правила поведінки, наявні у художніх творах, сприймаються й стають ціннісними життєвими орієнтаціями.
Caring Agent for the Call: The Lived Experience of Nurses as Call Center Agents
Authors: Cruz Jennalyn A Dela , Casis Bianca Louise C , Castillo Diana O. , Cruz Cruz , Gileen Tricia Adrienne C. , Doria Joyce D. , Gensaya Cathlaine Raye Gwyneth A. , Itable Raven Jeffsey B. , Rusiana Dejie M. , Bitera Ma. Xenia Z. , San Juan Estrella A. 
Published: June 2022

A number of registered nurses are working as call center agents instead of practicing their profession. This phenomenological inquiry aimed to understand and provide perspective on the lived experience of seven registered nurses, who worked as a call center agent for at least two years. Through intensive recorded interviews, the responses were initially clustered in ten. The initial five clusters were categorized into textural themes such a) ceasing opportunities vs chasing pavements; b) learning to adjust; c) deception and false images: seeing from inside out; d) reflection on the derailed career path ; e) self-fulfillment in the chosen path. The final five clusters were categorized into structural theme such as f) making ends meet; becoming the breadwinner; g) change vs tradition; h) gaining strength through challenges; i) eyes on the patient, ears on the phone; j) vulnerability of the strong. In conclusion, the lived experience of nurses working as call center agents provided self-fulfillment, through financial stability that enable them to provide for their family, personality and career growth, and the benefits received. They cope with the challenges encountered on this job by being competitive and having a strong personality, though two of the participants resorted to vices.
Factores de riesgo para infección urinaria por Escherichia coli BLEE positiva
Authors: Carcausto Huamani Eduardo , Rodríguez-Hurtado Diana 
Published: June 2022

Objetivo: establecer la asociación del uso previo de antibióticos, hospitalizaciones previas, infección urinaria previa, edad, sexo y comorbilidades en pacientes adultos hospitalizados con infección urinaria por Escherichia coli productora de beta lactamasas de espectro extendido (BLEE).
Material y métodos: estudio caso control, realizado en clínicas privadas de Lima en ámbito hospitalario. Se incluyeron 30 casos y 30 controles, definiéndose como caso al paciente hospitalizado que cuente con diagnóstico de infección urinaria por urocultivo de E. coli productora de BLEE y como control al paciente hospitalizado sin infección por E. coli BLEE. Se recolectó la información de casos incidentes. Se realizó un análisis bivariado y regresión logística multivariable con las variables que fueron significativas en el análisis bivariado.
Resultados: los factores asociados fueron: uso previo de antibióticos OR: 261 (22.5-11017.4), hospitalización previa OR: 4.6 (1.39-16.1), infección urinaria previa OR: 36 (6.9-227.2). Al ajustar por variables potencialmente confusoras mediante regresión logística, se observó que el principal factor asociado con significación estadística fue el uso previo de antibióticos, OR: 97.7 (8.4-1128.3, p<0.000).
Conclusión: se encontró evidencia de que el uso previo de antibióticos es un factor de riesgo asociado significativamente a infección urinaria por E. coli BLEE
Paradigms of US global strategy — concepts globalization and global instability
Authors: Senchenko Mykola 
Published: June 2022

The article considers the concepts of globalization and global instability, developed by think tanks in the United States and Great Britain. The encroachments of the Americans and the British on their own uniqueness have a long history. They began with the views of the first settlers — the Puritans, who arrived on the American continent in the seventeenth century, hoping to carry out the biblical commandment "a hill on a hill" where all the inhabitants are equal and free. Hence the substantiation of the concept of expansion of the principles of freedom and democracy as a manifestation of the logic of Divine Providence. It is one step from this thought to the idea of what determined America to reform the world and lead it.
Already in the middle of the XIX century аmerican ideologues argued that in the near future the United States would become a center around which all nations would unite into a single nation, that the United States, like the Sun, would have "a glorious influence on European countries and then on Asian empires".
The think tanks of some countries in the Asia-Pacific region
Authors: Senchenko Oksana 
Published: June 2022

The article provides a brief analysis of the brain centers of some countries in the Asia-Pacific region. It is shown how the analytical centers of these countries were transformed under the influence of liberalization and democratization. The object of attention of the brain centers of the countries of a certain region in general dimensions are the problems of state formation and processes outside the countries; activity, place, role and interaction of political parties, public organizations, movements and their leaders in the socio-political life of countries and their geopolitical position.
The aim of the article is to study the processes of formation and functioning of analytical centers in Kazakhstan, Taiwan, Turkey and Japan.