bluuebunny/crossref_metadata_2025_split huggingface dataset:
RBM3 is associated with acute lung injury in septic mice and patients via NF- κB/NLRP3 pathway
Authors: Long Feiyu , Hu Liren , Wang Yao , Duan Xiaoxia , Zhou Jun , Chen Guangxiang , Xie Keliang , Feng Jianguo , Wang Maohua 
Published: September 2022

Abstract
Sepsis refers to host response disorders caused by infection, which can lead to life-threatening organ dysfunction. And the lungs bear the brunt of the disease. RNA-binding motif protein 3 (RBM3) is an important cold-shock protein that is upregulated in response to mild hypothermia or hypoxia. In this study, we aimed to investigate whether RBM3 is involved in sepsis-associated acute lung injury (ALI). Intraperitoneal injection of LPS (10 mg/kg) was performed in wild-type (WT) and RBM3 knockout (KO, RBM3-/-) mice to establish an in vivo sepsis model. An NLRP3 inflammasome inhibitor, MCC950 (50 mg/kg), was injected intraperitoneally 30 min before LPS treatment, and serum, lung tissues, and BALF were collected 24 h later for further analysis. In addition, we also collected serum from sepsis patients and healthy volunteers to detect RBM3 expression. The results showed that the expression of RBM3 in the lung tissues of LPS-induced sepsis mice and the serum of patients with sepsis was significantly increased and positively correlated with disease severity. In addition, RBM3 knockout (KO) mice had a low survival rate, and RBM3 KO mice had more severe lung damage, inflammation, lung cell apoptosis, and oxidative stress than WT mice. After the LPS treatment, the levels of nucleotide binding and oligomerization domain-like receptor family 3 (NLRP3) inflammasomes and mononuclear cell nuclear factor-κB (NF-κB) in the lung tissues of RBM3 KO mice were significantly higher. However, they were only slightly elevated in WT mice. Interestingly, MCC950 improved LPS-induced acute lung injury in WT mice and RBM3 KO mice, and inhibited the expression of NLRP3, caspase-1, and IL-1β. In conclusion, RBM3 was overexpressed in sepsis patients and LPS-induced mice. The RBM3 gene deficiency aggravated sepsis-associated ALI through the NF-κB/NLRP3 pathway.
Real-time Quantification of Small Intestine Perfusion and Responses to Arterial Versus Venous Occlusion Using Laser Speckle Contrast Imaging in Porcine Intestinal Model
Authors: Liu Yao , Mehrotra Saloni , Nwaiwu Chibueze A , Buharin Vasiliy E , Oberlin John , Stolyarov Roman , Schwaitzberg Steven D , Kim Peter C.W. 
Published: September 2022

Abstract
Purpose: Real time intraoperative perfusion assessment may reduce anastomotic leaks. Laser Speckle Contrast Imaging (LSCI) provides dye-free visualization of perfusion by capturing coherent laser light scatter from red blood cells, and displays perfusion as a colormap. Herein, we report a novel method to precisely quantify intestinal perfusion using LSCI.Methods: ActivSightTM is a FDA-cleared multi-modal visualization system that can detect and display perfusion via both Indocyanine Green imaging (ICG) and LSCI in minimally invasive surgery. An experimental prototype LSCI perfusion quantification algorithm was evaluated in porcine models . Porcine small bowel was selectively devascularized to create regions of perfused/watershed/ischemic bowel and progressive aortic inflow/portal vein outflow clamping was performed to study arterial vs. venous ischemia. Continuous arterial pressure was monitored via femoral line.Results: LSCI perfusion colormaps and quantification distinguished between perfused, watershed, and ischemic bowel in all vascular control settings: no vascular occlusion (p = 1.52 x 10-15), aortic occlusion (p = 9.58 x 10-13), and portal venous occlusion (p = .00193). LSCI quantified similar levels of ischemia induced by states of arterial inflow and venous outflow occlusion. LSCI-quantified perfusion values correlated positively with higher mean arterial pressure and with increasing distance from ischemic bowel.Conclusion: LSCI relative perfusion quantification may provide more objective real-time assessment of intestinal perfusion, by quantifying currently subjective gradients of bowel ischemia and recognizing both arterial/venous etiologies of ischemia.
Different impacts of blood and sputum eosinophil counts on lung function and clinical outcomes in asthma: findings from the COREA cohort
Authors: Pham Duong Duc , Lee Ji-Hyang , Kim Ju-Young , An Jin , Song Woo-Jung , Kwon Hyouk-Soo , Cho You Sook , Kim Tae-Bum 
Published: September 2022

Abstract
Purpose Blood (EOS-B) and sputum (EOS-S) eosinophil counts may contribute differently to asthma pathogenesis. We compared the impact of the baseline EOS-B and EOS-S levels on lung function, asthma control, and exacerbation in Korean asthma patients.MethodsAsthma patients with baseline EOS-B (n=4257) and EOS-S (n=1049) levels from a multicenter cohort (COREA) were included. Pulmonary function test (%FEV1 predicted), asthma control test (ACT), and asthma exacerbation incidence were followed-up every 3 months for one year. Linear mixed effect models and survival analyses were used to examine the association between eosinophilic groups defined by EOS-B or EOS-S and outcomes. ResultsHigh eosinophilic groups were associated with a low baseline value and a high improvement in the %FEV1 predicted and ACT scores over time. The magnitude of group difference in %FEV1 predicted was 2-fold higher in the EOS-S versus EOS-B classification (mean and 95% CI: 4.7 (0.6-8.8) versus 2.0 (0.2-3.7) for the baseline value and -1.5 (-2.3 to -0.8) versus -0.8(-1.1 to -0.4) for the slope of change), whereas it was identical in ACT score. The magnitude of the impact increased linearly with the elevation of the cut-off level for the EOS-B but remained stable for the EOS-S classification. Patients with an elevation of both their EOS-B and EOS-S showed a higher increment in the %FEV1 predicted and ACT over time. Neither the EOS-B nor EOS-S was associated with asthma exacerbation. ConclusionEOS-S and EOS-B contribute differently to the clinical outcomes and should be taken into account independently to improve asthma care.
School-Based Harm Reduction with Adolescents: A Pilot Study
Authors: Fischer Nina Rose 
Published: September 2022

Abstract
A pilot study of the Safety First: Real Drug Education for Teens curriculum showed significant results pre to post with high school freshmen. Negative outcomes of drug education are linked to a failure to engage students because of developmentally inappropriate materials that include activities that have no relevance to the real experiences of young people. A few harm reduction studies showed significant increase in student's drug related knowledge. Students were less likely to consume substances, and less likely to consume to harmful levels. More studies are necessary to evidence harm reduction efficacy in the classroom. The goal of this study was to measure harm reduction knowledge and behaviors, including drug policy advocacy, before and after Safety First. Data were analyzed using AVOVA, linear regression, t-tests and thematic coding. Survey results, corroborated by the qualitative findings, showed a significant increase (p < .05) in high school freshmen harm reduction knowledge and behaviors in relationship to substance use pre to post Safety First. This increase related to a decrease in overall substance use. Harm reduction is often perceived as a controversial approach to substance use. These findings have implications for further study of what could be a promising harm reduction-based substance use intervention with teens.
Experimental Research on the Performance of a Novel Geo- filament Anchor for an Earthen Architectural Site
Authors: Yulan Wang , Jian Guo , weixi Zhang , Fan Lei 
Published: September 2022

Abstract
An efficient anchoring method, explicitly developed for small sliders, has hitherto been missing in the practice of earthen architecture conservation. Furthermore, anchorage performance studies conducted so far, have failed to fully take into account the soil characteristics of certain targets. To address these concerns, the conservation project conceived for the Gaochang Ruins, Turpan, in China, was selected as the experiment ground to design a novel Geotechnical Filament Anchor (GFA) for reinforcing small sliders in the earthen historical ramparts. In-situ experiments were conducted for evaluating six parameters—anchorage length (L), GF thickness (H), bore diameter (D), slurry strength (S), GFA surface status (R), and inclination angle (A). These parameters were varied in order to determine the effect they produce on anchorage performance, as demonstrated by the indicators, including tensile strength, destruction mode, load displacement (P-S) relation, and strain (ζ-L) distribution characteristics of the novel GFA. Data acquired from the experiments, in combination with the conservation specifics of earthen architectural sites, anchorage performance, and safety reserve, were further employed to introduce a calculation formula for computing the designed force value (N) through L. A simplified model depicting the shear stress distribution of the anchorage system under N was devised by extracting the strain distribution data with respect to the GF-slurry interface. Taking into account the soil properties of the above-mentioned site, the shear stress diffusion coefficient (α) was conceptualized, the formula for the shear strength of the grouting material was devised, and the tolerable ranges of L, D, H, R, and S were determined. A new design idea is proposed for the application of anchorage technology in earthen ruins in combination with the characteristics of cultural relics protection, which certainly promotes the formation of its design concept and calculation method.
Simulation of Wave Propagation with Obstacles: Time Invariance Operator Applied to Interference and Diffraction
Authors: Oliveira Marceliano , Oliveira Francisco , Freire Márcio , Valadares George 
Published: September 2022

Abstract
Computational Physics has been consolidated as an important field of study together with Basic Physics. In many cases the simulation is carried out considering an ideal case of a wave in infinite edge domain and in open space, not taking into account obstacles, barriers and other aspects of the real world. Our motivation is to perform wave simulation interacting with obstacles, barriers, single slit and double slit and investigate by examining the results obtained in images if the methodology
used by us to introduce realistic physical characteristics was successful presenting the expected phenomenology. We present a computational numerical operator that we call the Temporal Invariance Operator (TIO) this operator can add obstacles to the domain of the differential equation that describes a physical phenomenon, and after that, the wave equation can recognize these points as non-interacting zones without affecting the rest of the domain. The results in this paper allow us to infer that the operator TIO is an ideal way to apply the physical domain over wave propagation simulation and to successfully recreate wave interaction experiments with computer simulation.
Increasing access to piped water for poor households: an analysis of water connection subsidy projects implemented by the GRET
Authors: Michaels Terra , Suong Chanmeakara , Chi Chanrith , Morm Lyheang , Mab Phanit , Denis Justine 
Published: September 2022

Abstract
By 2030, Sustainable Development Goal 6 aims to ensure universal access to safe drinking water. The Royal Government of Cambodia has, thus, made a significant progress in improving the access to safe water supply in rural areas. However, results show only 26.9% of all poor households in a license area were connected when supported by the Groupe de Recherches et d'Echanges Technologique (GRET) programs compared to 45.6% of all households; nearly half of all poor households are located greater than 70 m or are of unknown location; and 56% of known households are within than 3 km. Logistic regression shows a negative relationship between household connections and increasing distance by 10 m, incremental water tariffs by 0.10 USD, and subsidized connection fees by 10 USD when compared to connections, with odds of connection decreasing in all cases (−23, −8.6, and −1%, respectively). Finally, 37% of unconnected households have never heard of the subsidy programs, indicating insufficient marketing programs. Recommendations include updating subsidies to cover all costs necessary for households to connect within 50 m of the distribution lines (an extra pipe, transaction fees, etc.); ensuring flat-rate connection fees paid by all poor households including all costs remaining 40 USD or less; subsidizing pipe extensions to reach unserved areas while maintaining fair tariffs for poor households and cost recoverability for piped water suppliers; shortening marketing and awareness programs to 3 months or less, with involvement from more local stakeholders; and adding poor household connection requirements to investment program requirements.
A Novel 6,8,9-Trisubstituted Purine Analogue Drives Breast Cancer Luminal A Subtype
MCF-7 to Apoptosis and Senescence through Hsp70 Inhibition
Authors: Tuncbilek Meral , Tutar Yusuf , Kul Pinar , Ergul Mustafa , Yenilmez Tunoglu Ezgi Nurdan 
Published: September 2022

Background:
Cancer cells restrain apoptotic and senescence pathways through intracellular heat shock
protein 70 (Hsp 70). These cells aid stimulus-independent growth, and their higher metabolism rate requires Hsps.
Hsps compensate abnormally increased substrate protein folding rate of cancer cells.

Objective:
Misfolding of substrate proteins especially signaling substrate proteins, may not function properly. Therefore,
Hsp70 folds these substrate proteins into their native-fully functional states, and this mode of action helps cancer
cell survival.

Method:
Targeting Hsps is promising cancer therapy, and in this study, 6,8,9-trisubstituted purine derivatives were
designed and synthesized to inhibit Hsp70 and drive cancer cells to apoptosis. Further, oncogenic stimuli through inhibitors
can induce an irreversible senescent state and senescence is a barrier to transformation.

Results:
Hsp70 helps cancer cells to bypass the cellular senescence program, however, binding of N6-(4-
isopropylaniline) analogue (7) depletes Hsp70 function as evidenced by aggregation assay and Hsp70 depletion induces
senescence pathway.

Conclusion:
The purine-based inhibitor-compound 7 effectively inhibits MCF-7 cell line. Moreover, the therapeutic
potential with regard to the senescence-associated secretory phenotype has complementary action. Dual action of the
inhibitor not only drives the cells to apoptosis but also force the cells to be in the senescence state and provides promising
results specially for luminal A type breast cancer therapy.
Comparison of learning civic(s) education in Indonesia with Japan
Authors: Andriyana Andriyana , Masyitoh Iim Siti 
Published: September 2022

Japan is a country with a quality education system and quality in the process of education and learning. Education in Japan implement standards and learning good management and structured coherently, including in the areas of learning Civic(s) Education. This study aims to provide comparative study Civic(s) Education in Indonesia with Japan. The method used in this research is a comparative study with a qualitative approach. The study was conducted in Bandung Japanese School. Informants consisted Japanese teacher of Civic(s) Education in Indonesia, Indonesian teacher of Civic(s) Education and Japanese lecturers in Indonesia. The collected data of this research obtained through interview, observation, literature and documentation then analyzed by analytical techniques such as data reduction, data presentation, and verification and validation using triangulation techniques and data collection techniques. The results showed that there are differences and similarities that exist in learning Civic(s) Education in Indonesia and Japan. Differences exist which of the methods used in the learning process, which in Indonesia using the method of lecture and discussion, while in Japan using a method that emphasizes living experience of learners in exploring the material obtained. And there are similarities in the structure of scientific disciplines Civic(s) Education in Indonesia and Japan are detached (separated) from the disciplines of social (Social Studies) and also in the purpose and role of the Civic(s) Education In shaping citizens' competence in the flow of globalization as well as moral education and character development of citizens.
Ki Hadjar Dewantara and Rabindranath Tagore’s thought about education
Authors: Supardi Supardi , Kuntoro Shodiq A , Dwiningrum Siti Irene Astuti 
Published: September 2022

This study aims to: (1) describe the struggle of Ki Hadjar Dewantara and Rabindranath Tagore in case to create the education; (2) find Ki Hadjar Dewantara’s and Rabindranath Tagore’s thought about education. This research was qualitative research, using historical approaches. Analysis of data was conducted in interactive way. This results show that, (1) Ki Hadjar Dewantara and Rabindranath against the colonial education system which were intellectual and hegemonic. Both developed education, that way Taman Siswa (Ki Hadjar) and Shantiniketan (Tagore). (2) Ki Hadjar and Tagore had a concept that education is power effort, guidance, to guide the growth of children. Principles of education of Ki Hadjar are reflected in principles of Taman Siswa and panca dharma Taman Siswa. Tagore saw education as a liberating process of obtaining the ultimate truth, which frees the shackles, provides a wealth of inner light, along with love. Ptinciples Tagore’s education was reflected in education institution, namely education in communion with nature, freedom, self expression, and internationalism and university.
"Be a Scientist" Learning media using adobe flash CS3 program containing the materials of the influence of islamic religion and culture in Indonesia for Grade X of vocational school
Authors: Sudrajat Ajat , Prasojo Lantip Diat , Zulkarnain Zulkarnain 
Published: September 2022

This research aims at investigating the needs for image media of "be a scientist" developed using Adobe Flash CS3 program containing the materials of the influence of religion and Islamic culture in Indonesia for learning History of grade X Vocational School and examining the feasibility of "be a Scientist" media developed using Adobe Flash CS3 program containing the materials of the influence of religion and Islamic culture in Indonesia which was developed to be used in the process of learning the history for grade X of Vocational school. This research employed Research and Development (R D) method. The product developed is in the form of "be a scientist" image media using Adobe Flash CS3 program that is appropriate for a learning resource in History subjects. The stages of research include: (1) the preliminary stage, (2) the planning stage of the model as the development of the initial product form, (3) the testing, evaluation and revision stages, and (4) the implementation stage or effectiveness test. Data were colected using FGD (Focus Group Discussion), questionnaires, interviews, observations, and documentation techniques. The validity of the instrument is achieved through expert judgement, while the validity of qualitative data is carried out using source triangulation techniques, theories and methods. Quantitative data analysis was performed using descriptive analysis techniques and qualitative data was carried out using an interactive models. The results show that: Adobe Flash CS 3 media images were needed in vocational schools for the development of history learning. The results of the assessment performed by the experts on material and media indicate that "be a scientist" image media using Adobe Flash CS3 program for history learning in Vocational High Schools has a good level of feasibility. This shows that the "be a Scientist" media using the Adobe Flash CS3 program contaiing the material of religious influence and Islamic culture in Indonesia is suitable for history learning in Vocational High Schools grade X.
Positive Affective Recovery in Daily Life as a Momentary Mechanism Across Subclinical and Clinical Stages of Mental Disorder: Experience Sampling Study
Authors: Ader Leonie , Schick Anita , Simons Claudia , Delespaul Philippe , Myin-Germeys Inez , Vaessen Thomas , Reininghaus Ulrich 
Published: September 2022

Background
Identifying momentary risk and protective mechanisms may enhance our understanding and treatment of mental disorders. Affective stress reactivity is one mechanism that has been reported to be altered in individuals with early and later stages of mental disorder. Additionally, initial evidence suggests individuals with early and enduring psychosis may have an extended recovery period of negative affect in response to daily stressors (ie, a longer duration until affect reaches baseline levels after stress), but evidence on positive affective recovery as a putative protective mechanism remains limited.

Objective
This study aimed to investigate trajectories of positive affect in response to stress across the continuum of mental disorder in a transdiagnostic sample.

Methods
Using the Experience Sampling Method, minor activity-, event-, and overall stress and positive affect were assessed 10 times a day, with time points approximately 90 minutes apart on six consecutive days in a pooled data set including 367 individuals with a mental disorder, 217 individuals at risk for a severe mental disorder, and 227 controls. Multilevel analysis and linear contrasts were used to investigate trajectories of positive affect within and between groups.

Results
Baseline positive affect differed across groups, and we observed stress reactivity in positive affect within each group. We found evidence for positive affective recovery after reporting activity- or overall stress within each group. While controls recovered to baseline positive affect about 90 minutes after stress, patients and at-risk individuals required about 180 minutes to recover. However, between-group differences in the affective recovery period fell short of significance (all P>.05).

Conclusions
The results provide first evidence that positive affective recovery may be relevant within transdiagnostic subclinical and clinical stages of mental disorder, suggesting that it may be a potential target for mobile health interventions fostering resilience in daily life.