bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of a Novel c.3080delC JAG1 Gene Mutation Associated With Alagille Syndrome: Whole Exome Sequencing
Authors: Panwar Deepak , Lal Vandana , Thatai Atul 
Published: April 2022

Background
Alagille syndrome is an autosomal dominant disorder associated with variable clinical phenotypic features including cholestasis, congenital heart defects, vertebral defects, and dysmorphic facies.

Objective
Whole exome sequencing (WES) has become technically feasible due to the recent advances in next-generation sequencing technologies, therefore offering new possibilities for mutations or genes identification.

Methods
WES was used to identify pathogenic variants, which may have significant prognostic implications for patients’ clinical presentation of the proband. In this paper, we have uncovered a novel JAGGED1 gene (JAG1) mutation associated with Alagille syndrome in a 5-year-old girl presented with conjugated hyperbilirubinemia and infantile cholestasis.

Results
The exome sequencing analysis revealed the presence of a novel JAG1 heterozygous c.3080delC variant in exon 25. The detected variant introduced a stop codon (p.P1027RfsTer9) in the gene sequence, encoding a truncated protein. Our exome observations were confirmed through Sanger sequencing as well.

Conclusions
Here, we report a case of a patient diagnosed with Alagille syndrome, conjugated hyperbilirubinemia, and infantile cholestasis, with emphasis on its association with the detection of the novel JAG1 mutation, thereby establishing the genetic diagnosis of the disease.
Emergency Telemedicine Mobile Ultrasounds Using a 5G-Enabled Application: Development and Usability Study
Authors: Berlet Maximilian , Vogel Thomas , Gharba Mohamed , Eichinger Joseph , Schulz Egon , Friess Helmut , Wilhelm Dirk , Ostler Daniel , Kranzfelder Michael 
Published: April 2022

Background
Digitalization affects almost every aspect of modern daily life, including a growing number of health care services along with telemedicine applications. Fifth-generation (5G) mobile communication technology has the potential to meet the requirements for this digitalized future with high bandwidths (10 GB/s), low latency (<1 ms), and high quality of service, enabling wireless real-time data transmission in telemedical emergency health care applications.

Objective
The aim of this study is the development and clinical evaluation of a 5G usability test framework enabling preclinical diagnostics with mobile ultrasound using 5G network technology.

Methods
A bidirectional audio-video data transmission between the ambulance car and hospital was established, combining both 5G-radio and -core network parts. Besides technical performance evaluations, a medical assessment of transferred ultrasound image quality and transmission latency was examined.

Results
Telemedical and clinical application properties of the ultrasound probe were rated 1 (very good) to 2 (good; on a 6 -point Likert scale rated by 20 survey participants). The 5G field test revealed an average end-to-end round trip latency of 10 milliseconds. The measured average throughput for the ultrasound image traffic was 4 Mbps and for the video stream 12 Mbps. Traffic saturation revealed a lower video quality and a slower video stream. Without core slicing, the throughput for the video application was reduced to 8 Mbps. The deployment of core network slicing facilitated quality and latency recovery.

Conclusions
Bidirectional data transmission between ambulance car and remote hospital site was successfully established through the 5G network, facilitating sending/receiving data and measurements from both applications (ultrasound unit and video streaming). Core slicing was implemented for a better user experience. Clinical evaluation of the telemedical transmission and applicability of the ultrasound probe was consistently positive.
The Impact of Perioperative Remote Patient Monitoring on Clinical Staff Workflows: Scoping Review
Authors: León Maria Alejandra , Pannunzio Valeria , Kleinsmann Maaike 
Published: April 2022

Background
Remote patient monitoring (RPM) interventions are being increasingly implemented in health care environments, given their benefits for different stakeholders. However, the effects of these interventions on the workflow of clinical staff are not always considered in RPM research and practice.

Objective
This review explored how contemporary RPM interventions affect clinical staff and their workflows in perioperative settings.

Methods
We conducted a scoping review of recent articles reporting the impact of RPM interventions implemented in perioperative settings on clinical staff and their workflow. The databases accessed were Embase and PubMed. A qualitative analysis was performed to identify the main problems and advantages that RPM brings to staff, in addition to the approaches taken to evaluate the impact of those interventions. Different themes were identified in terms of the challenges of RPM for clinical staff as well as in terms of benefits, risk-reduction strategies, and methods for measuring the impact of these interventions on the workflow of clinical staff.

Results
A total of 1063 papers were found during the initial search, of which 21 (1.98%) met the inclusion criteria. Of the 21 included papers, 15 (71%) focused on evaluating new RPM systems, 4 (19%) focused on existing systems, and 2 (10%) were reviews.

Conclusions
The reviewed literature shows that the impact on staff work experience is a crucial factor to consider when developing and implementing RPM interventions in perioperative settings. However, we noticed both underdevelopment and lack of standardization in the methods for assessing the impact of these interventions on clinical staff and their workflow. On the basis of the reviewed literature, we recommend the development of more robust methods for evaluating the impact of RPM interventions on staff experience in perioperative care; the adoption of a stronger focus on transition management when introducing these interventions in clinical practice; and the inclusion of longer periods of assessment, including the evaluation of long-term goals.
Construction of a Linked Data Set of COVID-19 Knowledge Graphs: Development and Applications
Authors: Wang Haofen , Du Huifang , Qi Guilin , Chen Huajun , Hu Wei , Chen Zhuo 
Published: April 2022

Background
With the continuous spread of COVID-19, information about the worldwide pandemic is exploding. Therefore, it is necessary and significant to organize such a large amount of information. As the key branch of artificial intelligence, a knowledge graph (KG) is helpful to structure, reason, and understand data.

Objective
To improve the utilization value of the information and effectively aid researchers to combat COVID-19, we have constructed and successively released a unified linked data set named OpenKG-COVID19, which is one of the largest existing KGs related to COVID-19. OpenKG-COVID19 includes 10 interlinked COVID-19 subgraphs covering the topics of encyclopedia, concept, medical, research, event, health, epidemiology, goods, prevention, and character.

Methods
In this paper, we introduce the key techniques exploited in building COVID-19 KGs in a top-down manner. First, the schema of the modeling process for each KG in OpenKG-COVID19 is described. Second, we propose different methods for extracting knowledge from open government sites, professional texts, public domain–specific sources, and public encyclopedia sites. The curated 10 COVID-19 KGs are further linked together at both the schema and data levels. In addition, we present the naming convention for OpenKG-COVID19.

Results
OpenKG-COVID19 has more than 2572 concepts, 329,600 entities, 513 properties, and 2,687,329 facts, and the data set will be updated continuously. Each COVID-19 KG was evaluated, and the average precision was found to be above 93%. We have developed search and browse interfaces and a SPARQL endpoint to improve user access. Possible intelligent applications based on OpenKG-COVID19 for further development are also described.

Conclusions
A KG is useful for intelligent question-answering, semantic searches, recommendation systems, visualization analysis, and decision-making support. Research related to COVID-19, biomedicine, and many other communities can benefit from OpenKG-COVID19. Furthermore, the 10 KGs will be continuously updated to ensure that the public will have access to sufficient and up-to-date knowledge.
Distinguishing Admissions Specifically for COVID-19 From Incidental SARS-CoV-2 Admissions: National Retrospective Electronic Health Record Study
Authors: Klann Jeffrey G , Strasser Zachary H , Hutch Meghan R , Kennedy Chris J , Marwaha Jayson S , Morris Michele , Samayamuthu Malarkodi Jebathilagam , Pfaff Ashley C , Estiri Hossein , South Andrew M , Weber Griffin M , Yuan William , Avillach Paul , Wagholikar Kavishwar B , Luo Yuan ,   The Consortium for Clinical Characterization of COVID-19 by EHR (4CE), Omenn Gilbert S , Visweswaran Shyam , Holmes John H , Xia Zongqi , Brat Gabriel A , Murphy Shawn N 
Published: April 2022

Background
Admissions are generally classified as COVID-19 hospitalizations if the patient has a positive SARS-CoV-2 polymerase chain reaction (PCR) test. However, because 35% of SARS-CoV-2 infections are asymptomatic, patients admitted for unrelated indications with an incidentally positive test could be misclassified as a COVID-19 hospitalization. Electronic health record (EHR)–based studies have been unable to distinguish between a hospitalization specifically for COVID-19 versus an incidental SARS-CoV-2 hospitalization. Although the need to improve classification of COVID-19 versus incidental SARS-CoV-2 is well understood, the magnitude of the problems has only been characterized in small, single-center studies. Furthermore, there have been no peer-reviewed studies evaluating methods for improving classification.

Objective
The aims of this study are to, first, quantify the frequency of incidental hospitalizations over the first 15 months of the pandemic in multiple hospital systems in the United States and, second, to apply electronic phenotyping techniques to automatically improve COVID-19 hospitalization classification.

Methods
From a retrospective EHR-based cohort in 4 US health care systems in Massachusetts, Pennsylvania, and Illinois, a random sample of 1123 SARS-CoV-2 PCR-positive patients hospitalized from March 2020 to August 2021 was manually chart-reviewed and classified as “admitted with COVID-19” (incidental) versus specifically admitted for COVID-19 (“for COVID-19”). EHR-based phenotyping was used to find feature sets to filter out incidental admissions.

Results
EHR-based phenotyped feature sets filtered out incidental admissions, which occurred in an average of 26% of hospitalizations (although this varied widely over time, from 0% to 75%). The top site-specific feature sets had 79%-99% specificity with 62%-75% sensitivity, while the best-performing across-site feature sets had 71%-94% specificity with 69%-81% sensitivity.

Conclusions
A large proportion of SARS-CoV-2 PCR-positive admissions were incidental. Straightforward EHR-based phenotypes differentiated admissions, which is important to assure accurate public health reporting and research.
Penularan Patogen CVPD Melalui Vektor D. citri Stadia Imago dan Nimfa pada Bibit Jeruk Rough Lemon dan Siem
Authors: Marlina Marlina , Mapegau Mapegau , Hayati Islah 
Published: April 2022

Penelitian ini bertujuanuntuk mempelajari penularan penyakit CVPD melaluivektor Diaphorina citri stadia imago dan nimfa pada jeruk Siem dan Rough Lemon. Percobaan dilaksanakan dengan menggunakan Rancangan Acak Lengkap pola faktorial dua faktor.Faktor Kultivar Jeruk (Rough Lemon, dan Siem) diletakkan sebagai faktor pertama, sedangkan fakor stadia vektor (Nimfa, dan Imago) sebagai faktor kedua. Empat kombinasi perlakukan masing-masing diulang 5 kali.Tiap perlakuan terdiri dari 2 pot tanaman percobaan.Data respons tanaman terhadap penyakit CVPD yang diinokulast melalul vektor D.citri berbeda stadia diperoleh dengan menghitung:1) masa inkubasi yang diperoleh dengan cara mengamati lamanya waktu yang diperlukan tanaman sejak diinokulasi patogen sampai timbulnya gejala pertama;2) persentase daun klorosis yang diperoleh melalui pengamatan daun sampel yang diambil secara acak dan dihitung jumlah daun yang klorosis dari jumlah daun tanaman sampel, 3) persentase cabang yang bergejala, diperoleh dengan menghitung jumlah cabang yang bergejala dari jumlah cabang seluruhnya per tanaman tiap perlakuan.Hasil penelitian menunjukkan bahwa masa inkubasi penyakit CVPD, persentase daun klorosis, dan persentase daun bergejala lebih cepat dan lebih tinggi pada jeruk Siem dibandingkan dengan pada jeruk Rough Lemon, baik yang ditulari melalui inokulasi dengan D. citri stadia imago maupun stadia nimfa. Tetapi penularan melalui inokulasi D. citri pada stadio imago lebih cepat memunculkan masa inkubasi, persentase daun, klorosis, dan persentase cabang bergejala lebih tinggi dibandingkan dengan stadia nimfa. Jeruk siem dan penularan penyakit CVPD melalui D. citri stadia nimfa dapat dijadikan cara deteksi dini penyakit CVPD pada jeruk
PROTEASE ACTIVITY OF THERMOPHILIC BACTERIA FROM LEJJA HOT SPRINGS IN SOPPENG SOUTH SULAWESI
Authors: Hafsan Hafsan , Ramadani Kurnia , Abbas Abbas 
Published: April 2022

This research aimed to observe quantitatively the effect of temperature and pH on protease activities from thermophilic bacteria collected from Lejjaâ€”Hot springs.Â Â Spectrophotometry on the casein substrate was used to test the proteolytic activity of the crude protease.Â Â Mixtures of enzyme and casein were incubated at various temperatures and pH for 20 minutes. The absorbance of tyrosine from protein hydrolysis was determined by spectrophotometry on Î» 280 nm. Temperature and pH impacted on protease activity were determined at temperature (60; 65; 70; 75; 80; 85 and 90oC) and pH (6.0; 6.5; 7.0; 7.5; 8, 0; 8,5 and 9,0). Results showed that treatment of temperature variations and pH had a significant effect on protease activity. Crude extract ofÂ Bacillus licheniformisÂ protease showed that the highest activity at 80oC and pH 7.5 was 0.1303 unit/ml/minute.Â BacillusÂ stearoformisÂ showed the highest enzymatic activity of the protease at 85oC, and pH 7.5 was 0.1226 Unit/ ml/minute. In comparison,Â Bacillus coagulansÂ reached optimum activity at 75oC, and pH 7.5 was 0.2052 Unit/ml/minute. Isolates ofÂ Bacillus licheniformis,Â B.Â coagulansÂ andÂ B. stearoformisÂ are bacteria that produce thermostable protease enzymes that can be developed as a source of genes and as a producer of the enzyme itself.
PENERAPAN POLINOMIAL ORDE-N DAN MALTHUSIAN PADA STUDI KASUS KAJIAN DAYA TAMPUNG WISATAWAN
Authors: Dalengkade Mario Nikolaus , Yusniar Masitah , Tjape Willyam , Boleu Fiktor Imanuel , Buka Oktosea 
Published: April 2022

Terletak di kawasan timur Indonesia, Tobelo Kabupaten Halmahera Utara terkenal dengan keindahan alamnya. Mamuya adalah destinasi wisata pemandian air panas yang memiliki keindahan alam. Pemandian air panas ini selalu ramai dikunjungi wisatawan lokal. Tapi pada faktanya destinasi mamuya belum dikelola berdasarkan ekologi. Luaran analisis menunjukan y=-0.179x^6+7.086x^5-109.0x^4+824.1x^3-3176x^2-5756x-2832Â dengan R2 = 0.793. Fungsi tersebut merepresentasikan kunjungan wisatawan, setiap bilangan yang bernilai positif mengindikasikan penambahan wisatawan. Sedangkan bilangan negatif merupakan kebalikan dari positif. Analisis daya dukung destinasi mamuya yakni 606.25 wisatawan per tahun sedangkan populasi maksimum kunjungan menyentuh angka 737.15 wisatawan per tahun. Data ini mengindikasikan terjadinya kelebihan kapasitas di kawasan wisata.
Partial Fourier Transform Methods to Solve the Solution Formula of Stokes Equation in Half-Space
Authors: Maryani Sri , Zahratunnisa Siti Fauziah , Sihwaningrum Idha , Wardayani Ari , Guswanto Bambang Hendriya 
Published: April 2022

Fluids are a shape of a matter which have substance liquids, gases and plasmas. In our daily life, fluids become important part, such as part of our blood and also help our body getting nutrients. It is well known that fluid motion can be described in mathematical model in especially in form of partial differential equations (PDE) and called as Navier Stokes EquationsÂ (NSE). The Navier Stokes equation is derived from balance of conservation of mass and conservation of momentum. In this paper, we consider the solution formula of the linearized of the Navier Stokes Equation (NSE) with the initial boundary value (IBV) problem in half space without surface tension. The model problem under consideration covers of non-linear fluid type. We solve the solution formula of velocity and density of the model problem by using Fourier transform and partial Fourier transform method. The strategy geting the solution of the model problem is based on the analysis of some resolvent of the model problem which obtained by using Laplace transform of the Stokes equations. Therefore, In particular, the formula of velocity and density of the Stokes equation are obtained.
PENENTUAN LAMA WAKTU OPTIMAL PADA PENGUKURAN GLUKOSA DARAH NON-INVASIF
Authors: Fitrianto Anwar , Erfiani Erfiani , Nisa Rahmatun 
Published: April 2022

Pengukuran kadar glukosa darah menggunakan metode invasif, yaitu melukai bagian tubuh, seperti jari, merupakan metode yang kurang disukai oleh sebagian besar masyarakat. Untuk itu, diperlukan pengembangan teknologi berupa alat pengukur kadar glukosa darah non-invasif. Alat ini menggunakan prinsip kerja spektroskopi inframerah. Oleh karena itu, lama waktu pengukuran menjadi hal yang harus dipertimbangkan. Keoptimalan lama waktu pengukuran diperlukan agar proses pemeriksaan kadar glukosa darah efisien dan bisa merekam seluruh informasi. Tujuan penelitian ini adalah menentukan lama waktu optimal pada alat pengukur kadar glukosa darah non-invasif. Data yang digunakan merupakan data primer hasil pengukuran kadar glukosa darah dari tiga responden. Data tersebut dianalisis menggunakan metode eksplorasi dan regresi linier. Hasil pemodelan dengan persamaan ,Â lama waktu optimal tersebut berada pada waktu perlakuan sebesar 1700 ms dengan menggunakan metode gradien pada kurva.
The relationship of the oil and gas fields of the Forecarpathian region with the regional faults system and deep structure
Authors: Starostenko V. I. , Murovskaya A.V. , Yegorova T.P. , Gintov O.B. , Amashukeli T.A. 
Published: April 2022

The oil and gas fields of Forecarpathian oil and gas region are compared with the structure of earth’s crust and regional faults. Most of the oil and gas condensate fields are localized in Borislav-Pokutia nappe over Precarpathian Fault zone. The majority of gas fields are located in under thrusting zone of the Sambir fault, which is a gently dipping detachment connected with the Forecarpathian fault and other faults of autochthonous basement. The regional gravity low of Carpathian Foredeep is also limited from northeast by the Sambir fault and from the southwest by the Uzhok fault. The Forecarpathian fault tends to the central part of gravity low. Transverse Tyachev-Nadvorna fault displaces the gravity low and delimits the oil and gas condensate fields. Velocity model along the wide-angle reflection and refraction PANCAKE profile shows a deep (25 km) sedimentary trough under the Carpathian Orogen. The Forecarpathian fault coincides with a jump of the Moho under the trough. Along the RomUkrSeis profile under Carpathian Orogen there were revealed a sedimentary trough as deep as 15 km and a low-velocity domain under the trough. From northeast, the trough is limited by the Forecarpathian fault, which goes up to the keel structure of the Moho discontinuity. Structure of the trough under Carpathian Orogen reflects development of East European Platform margin. The earliest stage of the formation of Baltica paleocontinent passive margin is seen in the structure of the trough’s lower part. Autochthonous basement under Borislav-Pokutsky and Skiba nappes includes a Riphean Massif limited by the Krakovets, Forecarpathian and Uzhok faults. The oil and gas condensate fields are located above the Riphean Massif.
The purpose of this article is to analyze the fault system and the structure of the earth’s crust in the Forecarpathian oil and gas region, as well as their relationship with the distribution of oil and gas deposits, taking into account new WARR profiles and other geological and geophysical data.
On the issue of material provision of personnel of the Civil service of special communication and information protection of Ukraine
Authors: Makovskyi D. V. 
Published: April 2022

The article considers the issue of material support of the personnel of the State Service for Special Communications and Information Protection of Ukraine. It is noted that the social security of its personnel has a significant impact on the quality and efficiency of the State Service for Special Communications and Information Protection of Ukraine. One of the areas of social protection is called material, which is that, in addition to the actual financial support in the form of wages, they can receive additional material support, which includes a number of allowances, surcharges, bonuses and compensations that can claim civil servants provided that they meet certain criteria specified in the legislation. It is noted that the material direction of social protection of this category of workers is characterized by the following features: it is realized in the form of allowances, surcharges, bonuses and compensations; the size of such types of social protection is either directly provided by law or determined directly by the head of the civil service; the types of material direction of social protection of employees, in fact, are components of their wages and are introduced in order to provide additional social protection given the functions they perform and the risks they face.
It is concluded that the material support of the personnel of the State Service for Special Communications and Information Protection of Ukraine consists of allowances, surcharges, bonuses and compensations, the amounts of which are directly provided by law or determined directly by the head of the civil service and are part of their salaries social protection. The signing by the President of Ukraine of the Decree on the decision of the National Security and Defense Council of Ukraine of October 22, 2021 "On the Concept of reforming the State Service for Special Communications and Information Protection of Ukraine" is a step in the right direction. and information protection of Ukraine needs improvement.