bluuebunny/crossref_metadata_2025_split huggingface dataset:
Obtaining the initial material for soybean breeding by physical mutagenesis
Authors: Davletov F. A. , Gainullina K. P. 
Published: June 2022

Relevance. Breeding of new highly productive, adaptive, technological soybean (Glycine max L.) cultivars plays an important role in increasing the fund of food protein. In this case, the key role belongs to the initial material. However the main problem of modern breeding has become a decrease in the genetic diversity of initial material of cultivated plants, including soybean. One of the ways to increase genetic polymorphism is the usage of induced mutagenesis. X-ray radiation is a highly effective physical mutagen that is successfully used in mutation breeding to increase the productivity of cultivated plants and acquire new traits by them. In this regard, the purpose of our work was to create a new initial material for soybean selection using ionizing radiation. The experiments were carried out in 2018–2020. The study materials were soybean cultivars SibNIIK 315, Milyausha, Chera 1, Samer 3, El’dorado, Zolotistaya.Methods. For induced mutagenesis, the air-dry seeds of the cultivars El’dorado and Zolotistaya distinguished in our studies by high productivity were exposed to X-ray radiation in doses of 105, 107, 1010 rad. After irradiation, the seeds were sown in the field. Field assessments, phenological observations were carried out according to the methodology of the State cultivar testing of agricultural crops.Results. The El’dorado cultivar showed less radiosensitivity. The largest number of mutant forms was obtained from it at the maximum dose of irradiation of seeds. The mutants were grouped according to their growing season duration and productivity. The group of highly productive early ripening mutant forms is of the greatest value for soybean breeding. Mutants MR-20/19, MR-22/19, MR-24/19, MR-28/19, MR-29/19 are 9–10% more productive than the original El’dorado cultivar and can be directly used to create new cultivars. Mutant forms from other groups identified by us are also of interest for soybean breeding.
Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats
Authors: Mudjihartini Ninik , Paramita Reni , Siregar Astrid Mariam Khairani , Filzadiyanti Estiana , Sarsanti Pungguri Ayu Nega , Purwaningsih Erni 
Published: June 2022

Background: Free radicals in excessive concentrations damages cells and accelerate the aging process. Antioxidants found in Centella asiatica (CA) and Acalypha indica (AI) have the potential to prevent oxidative cellular damage.
Objective: This study aimed to determine the effect of CA and AI on carbonyl and glutathione levels in the brain of older rats.
Methods: 18-month age rats were treated using either AI, CA, or vitamin E. In addition, 18-month age and 2-month age untreated rats were used as a negative control. The brain carbonyl and glutathione levels were measured by Agustyanak and the Elmann method, respectively.
Results: Treatment with CA significantly decreased brain carbonyl levels (2.87 nmol/mL) than the control rats (4.54 nmol/mL). Furthermore, treating AI did not reduce the brain carbonyl and GSH levels in aged brain rats.
Conclusion: Centella asiatica can reduce the protein destruction that occurs with increasing age.
THE CONTEXTS OF KYIV ROMANTICISM: ‘UKRAINIAN GERMAN’ MYKOLA HULAK
Authors: Borzenko Oleksandr 
Published: June 2022

The paper highlights the personal profile of the Cyril and Methodius Brotherhood member M. Hulak. The main focus is on studying his contribution to the development of Ukrainian romanticism. The tools of the theory of literary life allowed finding the optimal theoretical approach for systematization and comprehension of the available research materials. The biographical and cultural-aesthetic context plays an important role, emphasizing a number of essential aspects of the activity undertaken by the Cyril and Methodius Brotherhood. The research material also includes facts concerning education, social skills, and life principles that M. Hulak acquired in his university days. In the work, the study of Hulak’s education and national-cultural identity agrees with the understanding of his romantic interests, in particular his special interest in folk customary law and the peculiarities of the national character.
The paper aims to determine the main circle of Hulak’s Kyiv acquaintances, characterizes his important organizational, educational, and scholarly initiatives, clarifies the psychological motives of these actions. As far as it is possible, based on some facts and indirect evidence, the literary intentions of M. Hulak, not fully realized due to the arrests of the members of the Cyril and Methodius Brotherhood in 1847, have been analyzed. The cultural ideas adopted during his studies at the university, combined with romantic self-awareness, significantly determined the features of aesthetically marked behavior. The manifestations of romantic irony and grotesque were the key features of M. Hulak’s personal positioning in his communication with the investigators.
“THE PROSCRIBED”: CINEMATOGRAPHIC STUS
Authors: Punina Olha 
Published: June 2022

The paper reviews the image of Vasyl Stus in Roman Brovko’s feature film “The Proscribed” (“Zaboronenyi”, 2019). The researcher aims to find out how the director embodies the psychological structure of the writer’s personality in the film for a wide audience. To do this, the author of the paper uses interviews with the director, the leading actor, and contemporaries
of Vasyl Stus, analyzing the feature film from the perspective of its form, content, and meaning.
The members of the production team emphasized the reactions of the creative person to untruth and injustice; they focused on such traits of Stus’s character as being extremely honest, caring, and emotionally explosive. Stus’s contemporaries, watching the film first, noted the emotional side as successfully embodied on the screen.
The psychological component of Vasyl Stus’s cinematographic personality is based on the character traits, reactions, and behavior inherent to real Stus: the protagonist is prone to impetuous reactions, not suited to compromises, self-sufficient, and strong-willed. To create such a psychological structure of Vasyl Stus’s character in “The Proscribed”, the director works both with the form (he uses the principles of gradation and opposition, the means of image-experience, and a wide shot) and content (imaginary episodes, reading poetry behind the scenes, condensed meaning of the epigraph, some fictional characters, the author’s text in a new context, etc.). These efforts give the opportunity to see the movie character of Vasyl Stus with his inherent emotional reactions (impetuous) and feelings (protest, anger, justice), moral and ethical guidelines (to give much, to be humane and do good), and the high level of self-awareness.
INTERPRETATION OF THE MYSTERY GENRE IN THE PLAY “ELECTRIC TRAIN FOR EASTER” BY OLEKSANDR IRVANETS
Authors: Nikolaieva Oksana 
Published: June 2022

The paper focuses on the innovative reception of the archaic genre of mystery in the works by Oleksandr Irvanets. Based on the play “Th e Electric Train for Easter”, the researcher explores literary techniques that allow creatively transforming the gospel plot of Christ’s passions within the structure of modern drama. Special authorial interpretation of the mysterious matrix is studied in the context of complex tendencies that characterize the development of modern drama (actualization of mythological thinking, genre interaction, combination of styles). A number of modern researchers’ works give grounds for specifying the thematic and poetic features of the mystery genre.
Combining mundane life with sacred meanings, O. Irvanets’ play reveals genre convergence rather than diffusion. Due to the processes of convergence, the dramatic genre becomes colorful, stereoscopic, and polyphonic. Clearly realistic techniques used in the play border on elements of the theatre of the absurd and features of the symbolist drama.
The powerful tendencies of remythologization, being characteristic of the world and domestic drama of the 20th century, are noticeable in the drama “The Electric Train for Easter”, which combines elements of mystery, symbolist drama, and “drama of the absurd”.
These components coexist in the artistic system of the work on the principle of genre convergence, as each of them has its corresponding plotline. Th e most powerful mysterious element of the work determines a special concept of time: applying a linear temporal model to everyday scenes, the author clearly fixes in the dramatic action the point of transforming the profane time into sacred, cyclical, and related to the Passion of Christ. Th e theme of Jesus’ suffering is introduced through the biblical intertext, organically linked to the lines of the two central characters that may be seen as the modern invariant of the dialogue between Christ and Pontius Pilate.
Peran Kader dalam Penurunan Stunting di Desa
Authors: Ramadhan Kadar , Entoh Christina , Nurfatimah Nurfatimah 
Published: June 2022

Introduction: The government's seriousness in preventing stunting contained in the national strategy to accelerate stunting prevention should be appreciated. The inclusion of stunting as a priority problem in the use of village funds is the right step because the village is the spearhead, therefore in every village there must be health cadres who focus on this problem. Given the vital role of cadres, cadres need to be equipped with good knowledge. The purpose of this study was to increase the role of cadres in preventing stunting in Bulili Village, South Lore District. Methods: This study is a cohort study for 3 months with the target focus being posyandu cadres and the output is the nutritional status of TB/U of all children aged 0-23 months in Bulili Village, South Lore District. Results: the results showed that the efforts of cadres succeeded in reducing the prevalence of stunting from 35.3% in November 2018 to 16.7% in October 2019, exclusive breastfeeding was associated with stunting. Conclusion: Cadre assistance must be carried out continuously so that cadre performance remains good.

ABSTRAK
Pendahuluan: Keseriusan pemerintah dalam pencegahan stunting yang terdapat dalam strategi nasional percepatan pencegahan stunting patut diapresiasi. Dimasukannya stunting sebagai prioritas masalah dalam penggunan dana desa merupakan langkah tepat karena desa adalah ujung tombaknya, olehnya itu di setiap desa harus ada kader kesehatan yang fokus pada masalah ini. Mengingat peran kader yang vital maka kader perlu dibekali dengan pengetahuan yang baik. Tujuan penelitian ini adalah untuk meningkatkan peran kader dalam pencegahan stunting di Desa Bulili Kecamatan Lore Selatan. Metode: Penelitian ini merupakan penelitian cohort selama 3 bulan dengan fokus sasaran adalah kader posyandu dan outputnya adalah status gizi TB/U semua anak usia 0-23 bulan di Desa Bulili Kecamatan Lore Selatan. Hasil: hasil penelitian menunjukan usaha kader berhasil menurunkan prevalensi stunting dari 35,3% pada November 2018 menjadi 16,7% pada Oktober 2019, pemberian ASI eksklusif berhubungan dengan kejadian stunting. Simpulan: Pendampingan kader harus dilakukan berkelanjutan agar kinerja kader tetap baik.
In Situ Synthesis Mechanism and Photocatalytic Performance of Cyano-Bridged Cu (I)/Cu (II) Ultrathin Nanosheets
Authors: Li Shixiong , Qiang Jiawei , Lu Lifei , Yang Shaolong , Chen Yufeng , Liao Beiling 
Published: June 2022

In situ synthesis of cyano-bridged Cu (I)/Cu (II) complexes usually requires organometallic catalysts or is carried out under high-temperature and high-pressure conditions. Herein, the cyano-bridged two-dimensional Cu (I)/Cu (II) photocatalyst, [Cu2 (Py)3(CN)3]n (1), is synthesized in situ at room temperature. The in situ synthesis mechanism of 1 shows that the partial Cu (II) complex catalyzed the C-C bond cleavage of 1,3-isophthalonitrile (L) to introduce -CN and generate Cu (I)/Cu (II). Its ultrathin nanosheets can be obtained by adding sodium dodecyl benzene sulfonate and performing ultrasonic synthesis in the process of synthesis 1. The ultrathin nanosheets of 1 have a lattice distance of about 0.31 nm, and it can rapidly decompose methylene blue (MB) (K = 0.25 mg L−1 min−1 at pH = 3). This research work is beneficial for in situ synthesis of cyano-bridged Cu (I)/Cu (II) complexes at room temperature and explores their synthesis and photocatalytic mechanism.
Impact of Educational Interventions on Knowledge About Hypertensive Disorders of Pregnancy Among Pregnant Women: A Systematic Review
Authors: Gholami Kosar , Norouzkhani Narges , Kargar Meraj , Ghasemirad Hamidreza , Ashtiani Atieh Jafarabadi , Kiani Shamim , Sajedi Far Mahdi , Dianati Maryam , Salimi Yasaman , Khalaji Amirmohammad , Honari Sara , Deravi Niloofar 
Published: June 2022

BackgroundHypertensive disorders of pregnancy (HDP), including chronic hypertension, preeclampsia and gestational hypertension, is the cause of about 50,000 deaths out of 400,000 perinatal deaths. HDP is an effective risk factor in stroke, type 2 diabetes, and cardiovascular diseases like ischemic heart disease. There is a significant relation between HDP, lifestyle, and knowledge. Unfortunately, many studies showed that pregnant women have lack of knowledge about HDP. Therefore, the importance of educational interventions is, today, more acknowledged than before.AimThe goal of this systematic review was to investigate the effect of interventional educations on the knowledge of pregnant women about HDP.MethodsA systematic review of the related articles was conducted. We included English randomized controlled trials published up to December 2021, including pregnant women as population, HDP as the outcome, and educational interventions as the intervention.ResultsAfter the process of study selection, six articles containing 819 pregnant women were included in this study. Educational pamphlets, mobile-based application, a mixture of pamphlets, pictographic magnet and videos, and a combination of PowerPoint and data show projectors and conversation were the educational interventions in these studies.ConclusionsThe positive effects of educational interventions on the knowledge of women with HTP were observed in all studies. The higher knowledge leads to HDP-related complications.Systematic Review Registrationhttps://archive.org/details/osf-registrations-gcs5r-v1, identifier: doi: 10.17605/OSF.IO/GCS5R.
The Palma Echo Platform: Rationale and Design of an Echocardiography Core Lab
Authors: López Luis , Rossello Xavier , Romaguera Dora , Alonso-Gómez Ángel M. , Toledo Estefanía , Fortuny Elena , Noris Marta , Mas-Lladó Caterina , Fiol Miquel , Ramallal Raul , Tojal-Sierra Lucas , Alonso Alvaro , Fernandez-Palomeque Carlos 
Published: June 2022

BackgroundThe metabolic syndrome (MetS) is associated with increased cardiovascular morbidity and mortality. Characterization of cardiac structural and functional abnormalities due to the MetS can help recognize individuals who would benefit the most from preventive interventions. Transthoracic echocardiography (TTE) provides an opportunity to identify those abnormalities in a reproducible and cost-efficient manner. In research settings, implementation of protocols for the acquisition and analysis of TTE images are key to ensure validity and reproducibility, thus facilitating answering relevant questions about the association of the MetS with cardiac alterations.Methods and ResultsThe Palma Echo Platform (PEP) is a coordinated network that is built up to evaluate the underlying structural and functional cardiac substrate of participants with MetS. Repeated TTE will be used to evaluate 5-year changes in the cardiac structure and function in a group of 565 individuals participating in a randomized trial of a lifestyle intervention for the primary prevention of cardiovascular disease. The echocardiographic studies will be performed at three study sites, and will be centrally evaluated at the PEP core laboratory. Planned analyses will involve evaluating the effect of the lifestyle intervention on cardiac structure and function, and the association of the MetS and its components with changes in cardiac structure and function. Particular emphasis will be placed on evaluating parameters of left atrial structure and function, which have received more limited attention in past investigations. This PEP will be available for future studies addressing comparable questions.ConclusionIn this article we describe the protocol of a central echocardiography laboratory for the study of functional and structural alterations of the MetS.
A Pyroptosis-Related Signature Predicts Overall Survival and Immunotherapy Responses in Lung Adenocarcinoma
Authors: Zhu Kaibin , Yan An , Zhou Fucheng , Zhao Su , Ning Jinfeng , Yao Lei , Shang Desi , Chen Lantao 
Published: June 2022

Background: Lung adenocarcinoma (LUAD) is a highly malignant cancer with a bleak prognosis. Pyroptosis is crucial in LUAD. The present study investigated the prognostic value of a pyroptosis-related signature in LUAD.Methods: LUAD’s genomic data were downloaded from TCGA and GEO databases. K-means clustering was used to classify the data based on pyroptosis-related genes (PRGs). The features of tumor microenvironment were compared between the two subtypes. Differentially expressed genes (DEGs) were identified between the two subtypes, and functional enrichment and module analysis were carried out. LASSO Cox regression was used to build a prognostic model. Its prognostic value was assessed.Results: In LUAD, genetic and transcriptional changes in PRGs were found. A total of 30 PRGs were found to be differentially expressed in LUAD tissues. Based on PRGs, LUAD patients were divided into two subgroups. Subtype 1 has a higher overall survival rate than subtype 2. The tumor microenvironment characteristics of the two subtypes differed significantly. Compared to subtype 1, subtype 2 had strong immunological infiltration. Between the two groups, 719 DEGs were discovered. WGCNA used these DEGs to build a co-expression network. The network modules were analyzed. A prognostic model based on seven genes was developed, including FOSL1, KRT6A, GPR133, TMPRSS2, PRDM16, SFTPB, and SFTA3. The developed model was linked to overall survival and response to immunotherapy in patients with LUAD.Conclusion: In LUAD, a pyroptosis-related signature was developed to predict overall survival and treatment responses to immunotherapy.
Identification of Biomarkers Associated With CD8+ T Cells in Coronary Artery Disease and Their Pan-Cancer Analysis
Authors: Zhao Shijian , Wu Yinteng , Wei Yantao , Xu Xiaoyu , Zheng Jialin 
Published: June 2022

PurposeTo identify biomarkers associated with CD8+ T cells in coronary artery disease (CAD) and initially explore their potential role in the tumor immune microenvironment.Materials and MethodsCAD-related datasets GSE12288, GSE34198, and GSE66360, were downloaded from the GEO database. First, GSVA was performed based on the GSE12288 dataset. Then WGCNA analysis was performed to identify the most relevant module and candidate hub gene for CD8+ T cells, followed by GO and KEGG analysis of this module. Secondly, the relationship between candidate hub genes and CD8+ T cells was verified using GSE34198 and GSE66360, which led to the identification of hub genes. The relationship of hub genes with CD8+ T cells in cancer was analyzed using the TIMER database. Methylation analysis of hub genes was performed using the DiseaseMeth database. CAD, pan-cancer, pan-cell lines, and pan-normal tissues, correlations between hub genes. In addition, potential drugs and TFs associated with hub genes were predicted, and the ceRNA network was constructed. Finally, GSEA was performed separately for hub genes.ResultsCAD was shown to be associated with immune response by GSVA analysis. WGCNA identified the blue module as most related to CD8+ T cells and identified nine candidate hub genes. The relevance of CAD to immunity was further confirmed by GO and KEGG analysis of the module. Two additional datasets validated and identified three hub genes (FBXO7, RAD23A, and MKRN1) that significantly correlated with CD8+ T cells. In addition, we found that hub genes were positively associated with CD8+ T cells in TGCT, THCA, and KICH cancers by our analysis. Moreover, the hub gene was differentially methylated. We also analyzed the correlation between hub genes in CAD, different cancers, different cell lines, and different normal tissues. The results of all the analyses showed a positive correlation between them. Finally, we successfully constructed hub gene-associated TF-gene and ceRNA networks and predicted 11 drugs associated with hub genes. GSEA suggests that hub genes are related to multiple immune response processes.ConclusionFBXO7, RAD23A, and MKRN1 are significantly associated with CD8+ T cells in CAD and multiple cancers and may act through immune responses in CAD and cancer.
Comparison of an In Situ Imaging Device and Net-Based Method to Study Mesozooplankton Communities in an Oligotrophic System
Authors: Barth Alexander , Stone Joshua 
Published: June 2022

In the past several years, the capabilities of optical tools and in situ imaging devices have greatly expanded and are now revolutionizing the field of plankton research. These tools have facilitated the discovery of new plankton and enhanced the understanding of populations of fragile and gelatinous zooplankton. Imaging devices are becoming more accessible and regularly deployed on oceanographic studies and monitoring efforts. However, despite the increasing use of these tools, there are few studies which offer direct comparisons between in situ imaging devices and traditional-net based methods, especially in open-ocean, oligotrophic systems where plankton are sparser and less intensively sampled. This study compares estimates of mesozooplankton abundance calculated by net-tows and an Underwater Vision Profiler 5 (UVP5HD-DEEP) imaging system. Net tows were conducted with a Multiple Opening and Closing Nets with Environmental Sensing System (MOCNESS) device equipped with 153µm mesh. In total, four tows, each sampling eight distinct depth bins, were conducted aboard two cruises in the Sargasso Sea. Along each cruise, in situ images were collected using an Underwater Vison Profiler 5 (UVP5HD-DEEP). Using these methods, we estimated abundance of different mesozooplankton groups (&gt;0.5 mm). Using established biovolume-biomass conversions, we also estimated the dry mass of certain zooplankton taxa. Furthermore, we address two methods for calculating density and biomass concentration from UVP data. Estimates of mesozooplankton abundance and biomass concentration were generally higher from MOCNESS methods than the UVP estimates across all taxa. It was found that there is not a reliable relationship between UVP estimates and MOCNESS estimates when directly comparing similar depth bins. Nonetheless, when integrating density and biomass concentrations throughout the water column, estimates are not significantly different between the methodology. This study addresses several important considerations for using in situ imaging tools and how to reconcile findings with traditional net-based methods.